doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text	PSource	NProtein	NID	SiteSource	SiteName
31900356	5	0	theme	cleavage	876:883	arg1	site					885:888	The cleavage site	872:888	The cleavage site within the S2 subunit responsible for activation	872:937	The cleavage site within the S2 subunit responsible for activation also showed distinct structural features and glycosylation.					
31900356	4	1	theme	S	734:734	arg1	protein					736:742	FIPV S protein	729:742	FIPV S protein	729:742	Specifically, the N-glycans that wedge between 2 galectin-like domains within the S1 subunit of FIPV S protein result in a unique propeller-like conformation, underscoring the importance of glycosylation in maintaining protein structures.					
31900356	0	2	theme	unique	65:70	arg1	structure					72:80	a unique structure	63:80	a unique structure	63:80	Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.					
31900356	6	3	theme	FIP	1105:1107	arg1	pathogenesis					1089:1100	the pathogenesis	1085:1100	the pathogenesis of FIP	1085:1107	These structural insights provide a blueprint for a better molecular understanding of the pathogenesis of FIP.					
31900356	3	4	theme	distributed	477:487	arg1	N-glycans					519:527	densely distributed high-mannose and complex-type N-glycans	469:527	densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass	469:576	Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.					
31900356	0	5	theme	camouflaging	86:97	arg1	glycans					99:105	camouflaging glycans	86:105	camouflaging glycans	86:105	Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.					
31900356	2	6	theme	cryoelectron	267:278	arg1	cryo-EM					292:298	cryo-EM	292:298	cryo-EM	292:298	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	2	6	theme	cryoelectron	267:278	arg1	microscopy					280:289	a 3.3-Å cryoelectron microscopy	259:289	a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry	259:409	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	4	7	theme	S1	715:716	arg1	subunit					718:724	the S1 subunit	711:724	the S1 subunit of FIPV S protein	711:742	Specifically, the N-glycans that wedge between 2 galectin-like domains within the S1 subunit of FIPV S protein result in a unique propeller-like conformation, underscoring the importance of glycosylation in maintaining protein structures.					
31900356	4	8	theme	protein	852:858	arg1	structures					860:869	protein structures	852:869	protein structures	852:869	Specifically, the N-glycans that wedge between 2 galectin-like domains within the S1 subunit of FIPV S protein result in a unique propeller-like conformation, underscoring the importance of glycosylation in maintaining protein structures.					
31900356	2	9	theme	3.3-Å	261:265	arg1	cryo-EM					292:298	cryo-EM	292:298	cryo-EM	292:298	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	2	9	theme	3.3-Å	261:265	arg1	microscopy					280:289	a 3.3-Å cryoelectron microscopy	259:289	a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry	259:409	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	4	10	theme	galectin-like	682:694	arg1	domains					696:702	2 galectin-like domains	680:702	2 galectin-like domains within the S1 subunit of FIPV S protein	680:742	Specifically, the N-glycans that wedge between 2 galectin-like domains within the S1 subunit of FIPV S protein result in a unique propeller-like conformation, underscoring the importance of glycosylation in maintaining protein structures.					
31900356	3	11	theme	complex-type	506:517	arg1	N-glycans					519:527	densely distributed high-mannose and complex-type N-glycans	469:527	densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass	469:576	Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.					
31900356	2	12	dep	spike	334:338	arg1	S					341:341	S	341:341	S	341:341	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	6	13	theme	pathogenesis	1089:1100	arg1	understanding					1068:1080	a better molecular understanding	1049:1080	a better molecular understanding of the pathogenesis of FIP	1049:1107	These structural insights provide a blueprint for a better molecular understanding of the pathogenesis of FIP.					
31900356	2	14	theme	protein	344:350	arg1	structure					301:309	a 3.3-Å cryoelectron microscopy (cryo-EM) structure	259:309	a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry	259:409	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	2	15	theme	viral	399:403	arg1	entry					405:409	viral entry	399:409	viral entry	399:409	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	4	16	theme	glycosylation	823:835	arg1	importance					809:818	the importance	805:818	the importance of glycosylation in maintaining protein structures	805:869	Specifically, the N-glycans that wedge between 2 galectin-like domains within the S1 subunit of FIPV S protein result in a unique propeller-like conformation, underscoring the importance of glycosylation in maintaining protein structures.					
31900356	5	17	theme	responsible	912:922	arg1	subunit					904:910	the S2 subunit	897:910	the S2 subunit responsible for activation	897:937	The cleavage site within the S2 subunit responsible for activation also showed distinct structural features and glycosylation.					
31900356	0	18	theme	Cryo-EM	0:6	arg1	analysis					8:15	Cryo-EM analysis	0:15	Cryo-EM analysis of a feline coronavirus spike protein	0:53	Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.					
31900356	4	19	theme	FIPV	729:732	arg1	protein					736:742	FIPV S protein	729:742	FIPV S protein	729:742	Specifically, the N-glycans that wedge between 2 galectin-like domains within the S1 subunit of FIPV S protein result in a unique propeller-like conformation, underscoring the importance of glycosylation in maintaining protein structures.					
31900356	6	20	theme	molecular	1058:1066	arg1	understanding					1068:1080	a better molecular understanding	1049:1080	a better molecular understanding of the pathogenesis of FIP	1049:1107	These structural insights provide a blueprint for a better molecular understanding of the pathogenesis of FIP.					
31900356	3	21	theme	Mass	412:415	arg1	spectrometry					417:428	Mass spectrometry	412:428	Mass spectrometry	412:428	Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.					
31900356	2	22	theme	host	378:381	arg1	recognition					383:393	host recognition	378:393	host recognition	378:393	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	4	23	theme	protein	736:742	arg1	subunit					718:724	the S1 subunit	711:724	the S1 subunit of FIPV S protein	711:742	Specifically, the N-glycans that wedge between 2 galectin-like domains within the S1 subunit of FIPV S protein result in a unique propeller-like conformation, underscoring the importance of glycosylation in maintaining protein structures.					
31900356	2	24	theme	microscopy	280:289	arg1	structure					301:309	a 3.3-Å cryoelectron microscopy (cryo-EM) structure	259:309	a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry	259:409	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	5	25	theme	distinct	951:958	arg1	features					971:978	distinct structural features	951:978	distinct structural features	951:978	The cleavage site within the S2 subunit responsible for activation also showed distinct structural features and glycosylation.					
31900356	0	26	theme	coronavirus	29:39	arg1	protein					47:53	a feline coronavirus spike protein	20:53	a feline coronavirus spike protein	20:53	Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.					
31900356	3	27	theme	mass	573:576	arg1	mass					573:576	the total molecular mass	553:576	the total molecular mass	553:576	Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.					
31900356	3	27	theme	mass	573:576	arg1	1/4					546:548	1/4	546:548	1/4	546:548	Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.					
31900356	0	28	theme	feline	22:27	arg1	protein					47:53	a feline coronavirus spike protein	20:53	a feline coronavirus spike protein	20:53	Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.					
31900356	4	29	theme	propeller-like	763:776	arg1	conformation					778:789	a unique propeller-like conformation	754:789	a unique propeller-like conformation	754:789	Specifically, the N-glycans that wedge between 2 galectin-like domains within the S1 subunit of FIPV S protein result in a unique propeller-like conformation, underscoring the importance of glycosylation in maintaining protein structures.					
31900356	0	30	theme	protein	47:53	arg1	analysis					8:15	Cryo-EM analysis	0:15	Cryo-EM analysis of a feline coronavirus spike protein	0:53	Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.					
31900356	1	31	dep	rate	210:213	arg1	%					198:198	%	198:198	%	198:198	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	3	32	theme	total	557:561	arg1	mass					573:576	the total molecular mass	553:576	the total molecular mass	553:576	Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.					
31900356	1	33	theme	Feline	108:113	arg1	FIPV					145:148	FIPV	145:148	FIPV	145:148	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	1	33	theme	Feline	108:113	arg1	virus					138:142	Feline infectious peritonitis virus	108:142	Feline infectious peritonitis virus (FIPV)	108:149	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	1	33	theme	Feline	108:113	arg1	alphacoronavirus					157:172	an alphacoronavirus	154:172	an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment	154:241	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	0	34	theme	spike	41:45	arg1	protein					47:53	a feline coronavirus spike protein	20:53	a feline coronavirus spike protein	20:53	Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.					
31900356	3	35	theme	molecular	563:571	arg1	mass					573:576	the total molecular mass	553:576	the total molecular mass	553:576	Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.					
31900356	1	36	theme	infectious	115:124	arg1	FIPV					145:148	FIPV	145:148	FIPV	145:148	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	1	36	theme	infectious	115:124	arg1	virus					138:142	Feline infectious peritonitis virus	108:142	Feline infectious peritonitis virus (FIPV)	108:149	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	1	36	theme	infectious	115:124	arg1	alphacoronavirus					157:172	an alphacoronavirus	154:172	an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment	154:241	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	1	37	theme	peritonitis	126:136	arg1	FIPV					145:148	FIPV	145:148	FIPV	145:148	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	1	37	theme	peritonitis	126:136	arg1	virus					138:142	Feline infectious peritonitis virus	108:142	Feline infectious peritonitis virus (FIPV)	108:149	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	1	37	theme	peritonitis	126:136	arg1	alphacoronavirus					157:172	an alphacoronavirus	154:172	an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment	154:241	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	1	38	theme	mortality	200:208	arg1	rate					210:213	a nearly 100% mortality rate	186:213	a nearly 100% mortality rate	186:213	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	3	39	theme	N-glycans	519:527	arg1	compositions					453:464	site-specific compositions	439:464	site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass	439:576	Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.					
31900356	1	40	dep	%	198:198	arg1	100					195:197	100	195:197	100	195:197	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
31900356	3	41	theme	site-specific	439:451	arg1	compositions					453:464	site-specific compositions	439:464	site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass	439:576	Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.					
31900356	5	42	theme	S2	901:902	arg1	subunit					904:910	the S2 subunit	897:910	the S2 subunit responsible for activation	897:937	The cleavage site within the S2 subunit responsible for activation also showed distinct structural features and glycosylation.					
31900356	5	43	theme	structural	960:969	arg1	features					971:978	distinct structural features	951:978	distinct structural features	951:978	The cleavage site within the S2 subunit responsible for activation also showed distinct structural features and glycosylation.					
31900356	4	44	theme	unique	756:761	arg1	conformation					778:789	a unique propeller-like conformation	754:789	a unique propeller-like conformation	754:789	Specifically, the N-glycans that wedge between 2 galectin-like domains within the S1 subunit of FIPV S protein result in a unique propeller-like conformation, underscoring the importance of glycosylation in maintaining protein structures.					
31900356	6	45	theme	better	1051:1056	arg1	understanding					1068:1080	a better molecular understanding	1049:1080	a better molecular understanding of the pathogenesis of FIP	1049:1107	These structural insights provide a blueprint for a better molecular understanding of the pathogenesis of FIP.					
31900356	3	46	theme	high-mannose	489:500	arg1	N-glycans					519:527	densely distributed high-mannose and complex-type N-glycans	469:527	densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass	469:576	Mass spectrometry provided site-specific compositions of densely distributed high-mannose and complex-type N-glycans that account for 1/4 of the total molecular mass; most of the N-glycans could be visualized by cryo-EM.					
31900356	6	47	theme	structural	1005:1014	arg1	insights					1016:1023	These structural insights	999:1023	These structural insights	999:1023	These structural insights provide a blueprint for a better molecular understanding of the pathogenesis of FIP.					
31900356	2	48	theme	spike	334:338	arg1	protein					344:350	the serotype I FIPV spike (S) protein	314:350	the serotype I FIPV spike (S) protein	314:350	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	2	48	theme	spike	334:338	arg1	responsible					362:372	responsible	362:372	responsible	362:372	Here we report a 3.3-Å cryoelectron microscopy (cryo-EM) structure of the serotype I FIPV spike (S) protein, which is responsible for host recognition and viral entry.					
31900356	1	49	theme	effective	223:231	arg1	treatment					233:241	effective treatment	223:241	effective treatment	223:241	Feline infectious peritonitis virus (FIPV) is an alphacoronavirus that causes a nearly 100% mortality rate without effective treatment.					
32856761	2	0	theme	sugar	517:521	arg1	reactivity					489:498	the reactivity	485:498	the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter	485:622	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	3	1	from	glycosylations	752:765	arg1	combination					770:780	combination	770:780	combination with the detection and characterization of different reactive intermediates using experimental and computational techniques	770:904	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	1	2	theme	constituting	283:294	arg1	blocks					305:310	the constituting building blocks	279:310	the constituting building blocks	279:310	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	2	3	theme	bacterial	507:515	arg1	caryophyllose					524:536	caryophyllose	524:536	caryophyllose	524:536	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	2	3	theme	bacterial	507:515	arg1	sugar					517:521	the bacterial sugar	503:521	the bacterial sugar	503:521	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	2	3	theme	bacterial	507:515	arg1	C12-monosaccharide					546:563	a rare C12-monosaccharide	539:563	a rare C12-monosaccharide	539:563	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	4	4	theme	blocks	988:993	arg1	design					969:974	the rational design	956:974	the rational design of building blocks with the required properties	956:1022	The insights garnered from these studies enabled the rational design of building blocks with the required properties to assemble mycobacterial lipooligosaccharide fragments of M. marinum.					
32856761	4	5	with	design	969:974	arg1	properties					1013:1022	the required properties	1000:1022	the required properties	1000:1022	The insights garnered from these studies enabled the rational design of building blocks with the required properties to assemble mycobacterial lipooligosaccharide fragments of M. marinum.					
32856761	1	6	theme	building	296:303	arg1	blocks					305:310	the constituting building blocks	279:310	the constituting building blocks	279:310	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	4	7	theme	required	1004:1011	arg1	properties					1013:1022	the required properties	1000:1022	the required properties	1000:1022	The insights garnered from these studies enabled the rational design of building blocks with the required properties to assemble mycobacterial lipooligosaccharide fragments of M. marinum.					
32856761	1	8	theme	blocks	305:310	arg1	reactivity					265:274	the reactivity	261:274	the reactivity of the constituting building blocks	261:310	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	1	8	theme	blocks	305:310	arg1	stereoselectivity					320:336	the stereoselectivity	316:336	the stereoselectivity of the reactions in which they partake	316:375	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	2	9	theme	rare	541:544	arg1	sugar					517:521	the bacterial sugar	503:521	the bacterial sugar	503:521	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	2	9	theme	rare	541:544	arg1	C12-monosaccharide					546:563	a rare C12-monosaccharide	539:563	a rare C12-monosaccharide	539:563	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	3	10	theme	different	825:833	arg1	intermediates					844:856	different reactive intermediates	825:856	different reactive intermediates using experimental and computational techniques	825:904	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	2	11	contain	containing	566:575	arg2	stereocenter					611:622	a characteristic tetrasubstituted stereocenter	577:622	a characteristic tetrasubstituted stereocenter	577:622	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	2	11	contain	containing	566:575	arg1	sugar					517:521	the bacterial sugar	503:521	the bacterial sugar	503:521	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	2	11	contain	containing	566:575	arg1	C12-monosaccharide					546:563	a rare C12-monosaccharide	539:563	a rare C12-monosaccharide	539:563	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	4	12	theme	rational	960:967	arg1	design					969:974	the rational design	956:974	the rational design of building blocks with the required properties	956:1022	The insights garnered from these studies enabled the rational design of building blocks with the required properties to assemble mycobacterial lipooligosaccharide fragments of M. marinum.					
32856761	3	13	dep	detection	791:799	arg1	the					787:789	the	787:789	the	787:789	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	4	14	theme	building	979:986	arg1	blocks					988:993	building blocks	979:993	building blocks	979:993	The insights garnered from these studies enabled the rational design of building blocks with the required properties to assemble mycobacterial lipooligosaccharide fragments of M. marinum.					
32856761	0	15	theme	Reactivity-Stereoselectivity	0:27	arg1	Mapping					29:35	Reactivity-Stereoselectivity Mapping	0:35	Reactivity-Stereoselectivity Mapping for the Assembly of Mycobacterium marinum Lipooligosaccharides.	0:99	Reactivity-Stereoselectivity Mapping for the Assembly of Mycobacterium marinum Lipooligosaccharides.					
32856761	1	16	theme	complex	117:123	arg1	glycans					135:141	complex bacterial glycans	117:141	complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages	117:203	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	3	17	theme	systematic	731:740	arg1	series					742:747	a systematic series	729:747	a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques	729:904	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	1	18	theme	stereoselectivity	320:336	arg1	knowledge					248:256	knowledge	248:256	knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake	248:375	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	2	19	theme	characteristic	579:592	arg1	stereocenter					611:622	a characteristic tetrasubstituted stereocenter	577:622	a characteristic tetrasubstituted stereocenter	577:622	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	1	20	theme	bacterial	125:133	arg1	glycans					135:141	complex bacterial glycans	117:141	complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages	117:203	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	3	21	theme	reactive	835:842	arg1	intermediates					844:856	different reactive intermediates	825:856	different reactive intermediates using experimental and computational techniques	825:904	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	4	22	theme	lipooligosaccharide	1050:1068	arg1	fragments					1070:1078	mycobacterial lipooligosaccharide fragments	1036:1078	mycobacterial lipooligosaccharide fragments of M. marinum	1036:1092	The insights garnered from these studies enabled the rational design of building blocks with the required properties to assemble mycobacterial lipooligosaccharide fragments of M. marinum.					
32856761	1	23	theme	glycans	135:141	arg1	assembly					105:112	The assembly	101:112	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages	101:203	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	2	24	theme	blocks	451:456	arg1	reactivity					415:424	the reactivity	411:424	the reactivity of carbohydrate building blocks	411:456	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	3	25	from	combination	770:780	arg1	series					742:747	a systematic series	729:747	a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques	729:904	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	3	26	theme	intermediates	844:856	arg1	characterization					805:820	characterization	805:820	characterization	805:820	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	3	26	theme	intermediates	844:856	arg1	detection					791:799	detection	791:799	detection	791:799	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	4	27	theme	M.	1083:1084	arg1	marinum					1086:1092	M. marinum	1083:1092	M. marinum	1083:1092	The insights garnered from these studies enabled the rational design of building blocks with the required properties to assemble mycobacterial lipooligosaccharide fragments of M. marinum.					
32856761	1	28	theme	knowledge	248:256	arg1	lack					240:243	the lack	236:243	the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake	236:375	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	2	29	theme	building	442:449	arg1	blocks					451:456	carbohydrate building blocks	429:456	carbohydrate building blocks	429:456	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	1	30	theme	reactions	345:353	arg1	reactivity					265:274	the reactivity	261:274	the reactivity of the constituting building blocks	261:310	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	1	30	theme	reactions	345:353	arg1	stereoselectivity					320:336	the stereoselectivity	316:336	the stereoselectivity of the reactions in which they partake	316:375	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	2	31	theme	carbohydrate	429:440	arg1	blocks					451:456	carbohydrate building blocks	429:456	carbohydrate building blocks	429:456	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	0	32	theme	marinum	71:77	arg1	Lipooligosaccharides					79:98	Mycobacterium marinum Lipooligosaccharides	57:98	Mycobacterium marinum Lipooligosaccharides	57:98	Reactivity-Stereoselectivity Mapping for the Assembly of Mycobacterium marinum Lipooligosaccharides.					
32856761	3	33	theme	experimental	864:875	arg1	techniques					895:904	experimental and computational techniques	864:904	experimental and computational techniques	864:904	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	0	34	theme	Mycobacterium	57:69	arg1	Lipooligosaccharides					79:98	Mycobacterium marinum Lipooligosaccharides	57:98	Mycobacterium marinum Lipooligosaccharides	57:98	Reactivity-Stereoselectivity Mapping for the Assembly of Mycobacterium marinum Lipooligosaccharides.					
32856761	3	35	theme	glycosylations	752:765	arg1	series					742:747	a systematic series	729:747	a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques	729:904	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	3	36	theme	reactivity-stereoselectivity	635:662	arg1	relationships					664:676	reactivity-stereoselectivity relationships	635:676	reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides	635:724	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	1	37	theme	rare	154:157	arg1	motifs					170:175	rare structural motifs	154:175	rare structural motifs	154:175	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	3	38	theme	computational	881:893	arg1	techniques					895:904	experimental and computational techniques	864:904	experimental and computational techniques	864:904	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	4	39	theme	mycobacterial	1036:1048	arg1	fragments					1070:1078	mycobacterial lipooligosaccharide fragments	1036:1078	mycobacterial lipooligosaccharide fragments of M. marinum	1036:1092	The insights garnered from these studies enabled the rational design of building blocks with the required properties to assemble mycobacterial lipooligosaccharide fragments of M. marinum.					
32856761	1	40	theme	structural	159:168	arg1	motifs					170:175	rare structural motifs	154:175	rare structural motifs	154:175	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	2	41	theme	tetrasubstituted	594:609	arg1	stereocenter					611:622	a characteristic tetrasubstituted stereocenter	577:622	a characteristic tetrasubstituted stereocenter	577:622	We here report a strategy to map the reactivity of carbohydrate building blocks and apply it to understand the reactivity of the bacterial sugar, caryophyllose, a rare C12-monosaccharide, containing a characteristic tetrasubstituted stereocenter.					
32856761	3	42	theme	acceptor	706:713	arg1	glycosides					715:724	caryophyllose donor and acceptor glycosides	682:724	caryophyllose donor and acceptor glycosides	682:724	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	1	43	theme	reactivity	265:274	arg1	knowledge					248:256	knowledge	248:256	knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake	248:375	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	3	44	from	series	742:747	arg1	combination					770:780	combination	770:780	combination with the detection and characterization of different reactive intermediates using experimental and computational techniques	770:904	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	3	45	theme	caryophyllose	682:694	arg1	donor					696:700	caryophyllose donor	682:700	caryophyllose donor	682:700	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	0	46	theme	Lipooligosaccharides	79:98	arg1	Assembly					45:52	the Assembly	41:52	the Assembly of Mycobacterium marinum Lipooligosaccharides	41:98	Reactivity-Stereoselectivity Mapping for the Assembly of Mycobacterium marinum Lipooligosaccharides.					
32856761	3	47	theme	donor	696:700	arg1	glycosides					715:724	caryophyllose donor and acceptor glycosides	682:724	caryophyllose donor and acceptor glycosides	682:724	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	1	48	theme	cis-glycosidic	181:194	arg1	linkages					196:203	cis-glycosidic linkages	181:203	cis-glycosidic linkages	181:203	The assembly of complex bacterial glycans presenting rare structural motifs and cis-glycosidic linkages is significantly obstructed by the lack of knowledge of the reactivity of the constituting building blocks and the stereoselectivity of the reactions in which they partake.					
32856761	3	49	with	combination	770:780	arg1	characterization					805:820	characterization	805:820	characterization	805:820	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	3	49	with	combination	770:780	arg1	detection					791:799	detection	791:799	detection	791:799	We mapped reactivity-stereoselectivity relationships for caryophyllose donor and acceptor glycosides by a systematic series of glycosylations in combination with the detection and characterization of different reactive intermediates using experimental and computational techniques.					
32856761	4	50	theme	marinum	1086:1092	arg1	fragments					1070:1078	mycobacterial lipooligosaccharide fragments	1036:1078	mycobacterial lipooligosaccharide fragments of M. marinum	1036:1092	The insights garnered from these studies enabled the rational design of building blocks with the required properties to assemble mycobacterial lipooligosaccharide fragments of M. marinum.					
32126781	5	0	theme	glycosidic	799:808	arg1	topology					810:817	glycosidic topology	799:817	glycosidic topology of the oligosaccharide group	799:846	For glycopeptide analysis, CID is best known for the ability to determine glycosidic topology of the oligosaccharide group; however, CID has also been shown to produce amide bond cleavage of the polypeptide group.					
32126781	7	1	theme	O-linked	1231:1238	arg1	glycopeptides					1240:1252	O-linked glycopeptides	1231:1252	O-linked glycopeptides	1231:1252	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	7	2	theme	several	1146:1152	arg1	studies					1154:1160	several studies	1146:1160	several studies on N-linked glycopeptides	1146:1186	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	3	3	theme	peptide	558:564	arg1	connectivity					499:510	the monosaccharide connectivity	480:510	the monosaccharide connectivity of the glycan	480:524	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	3	theme	peptide	558:564	arg1	sequence					542:549	the amino acid sequence	527:549	the amino acid sequence of the peptide	527:564	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	3	theme	peptide	558:564	arg1	site					575:578	the site	571:578	the site of glycosylation for each glycopeptide of interest	571:629	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	3	theme	peptide	558:564	arg1	glycan					519:524	the glycan	515:524	the glycan	515:524	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	3	theme	peptide	558:564	arg1	glycosylation					583:595	glycosylation	583:595	glycosylation	583:595	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	3	theme	peptide	558:564	arg1	peptide					558:564	the peptide	554:564	the peptide	554:564	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	1	4	theme	mass	298:301	arg1	spectrometry					303:314	tandem mass spectrometry	291:314	tandem mass spectrometry (MS/MS)	291:322	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32126781	1	4	theme	mass	298:301	arg1	MS/MS					317:321	MS/MS	317:321	MS/MS	317:321	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32126781	7	5	theme	fragmentation	1098:1110	arg1	pathways					1112:1119	these energy-resolved fragmentation pathways	1076:1119	these energy-resolved fragmentation pathways	1076:1119	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	7	5	theme	fragmentation	1098:1110	arg1	subject					1135:1141	the subject	1131:1141	the subject of several studies on N-linked glycopeptides	1131:1186	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	7	6	from	glycopeptides	1174:1186	arg1	pathways					1112:1119	these energy-resolved fragmentation pathways	1076:1119	these energy-resolved fragmentation pathways	1076:1119	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	7	6	from	glycopeptides	1174:1186	arg1	subject					1135:1141	the subject	1131:1141	the subject of several studies on N-linked glycopeptides	1131:1186	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	7	7	theme	studies	1154:1160	arg1	pathways					1112:1119	these energy-resolved fragmentation pathways	1076:1119	these energy-resolved fragmentation pathways	1076:1119	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	7	7	theme	studies	1154:1160	arg1	subject					1135:1141	the subject	1131:1141	the subject of several studies on N-linked glycopeptides	1131:1186	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	5	8	gly	glycopeptide	729:740	arg2	glycopeptide					729:740	glycopeptide analysis	729:749	glycopeptide analysis	729:749	For glycopeptide analysis, CID is best known for the ability to determine glycosidic topology of the oligosaccharide group; however, CID has also been shown to produce amide bond cleavage of the polypeptide group.					
32126781	0	9	theme	O-Glycopeptides	136:150	arg1	Dissociation					109:120	Energy-Resolved Collison-Induced Dissociation	76:120	Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides	76:150	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides Derived from Nonspecific Proteolysis.					
32126781	9	10	gly	O-glycopeptides	1527:1541	arg2	O-glycopeptides					1527:1541	O-glycopeptides	1527:1541	O-glycopeptides	1527:1541	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	3	11	theme	glycosylation	583:595	arg1	connectivity					499:510	the monosaccharide connectivity	480:510	the monosaccharide connectivity of the glycan	480:524	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	11	theme	glycosylation	583:595	arg1	sequence					542:549	the amino acid sequence	527:549	the amino acid sequence of the peptide	527:564	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	11	theme	glycosylation	583:595	arg1	site					575:578	the site	571:578	the site of glycosylation for each glycopeptide of interest	571:629	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	11	theme	glycosylation	583:595	arg1	glycan					519:524	the glycan	515:524	the glycan	515:524	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	11	theme	glycosylation	583:595	arg1	glycosylation					583:595	glycosylation	583:595	glycosylation	583:595	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	11	theme	glycosylation	583:595	arg1	peptide					558:564	the peptide	554:564	the peptide	554:564	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	6	12	theme	collision	1052:1060	arg1	energy					1062:1067	the applied collision energy	1040:1067	the applied collision energy	1040:1067	Whether structural information is obtained for the glycan or the peptide has been found to depend on the applied collision energy.					
32126781	9	13	theme	previous	1452:1459	arg1	findings					1461:1468	previous findings	1452:1468	previous findings	1452:1468	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	0	14	theme	Protonated	125:134	arg1	O-Glycopeptides					136:150	Protonated O-Glycopeptides	125:150	Protonated O-Glycopeptides	125:150	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides Derived from Nonspecific Proteolysis.					
32126781	4	15	gly	glycoprotein	711:722	arg1	glycoprotein					711:722	the intact glycoprotein	700:722	the intact glycoprotein	700:722	In turn, this allows inferences with respect to the glycoprofile of the intact glycoprotein.					
32126781	8	16	theme	energy-dependent	1396:1411	arg1	manner					1413:1418	an energy-dependent manner	1393:1418	an energy-dependent manner	1393:1418	In this study, MS/MS via CID was shown to provide substantial peptide backbone fragmentation, in addition to glycosidic fragmentation, in an energy-dependent manner.					
32126781	1	17	theme	Collision-induced	190:206	arg1	method					275:280	the most broadly applied dissociation method	237:280	the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS)	237:322	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32126781	1	17	theme	Collision-induced	190:206	arg1	CID					222:224	CID	222:224	CID	222:224	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32126781	1	17	theme	Collision-induced	190:206	arg1	dissociation					208:219	Collision-induced dissociation	190:219	Collision-induced dissociation (CID)	190:225	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32126781	11	18	gly	O-glycopeptide	1786:1799	arg2	O-glycopeptide					1786:1799	O-glycopeptide ER-CID	1786:1806	O-glycopeptide ER-CID	1786:1806	Establishing a more complete understanding of O-glycopeptide ER-CID is likely to have a substantive impact on how O-glycoproteomic analysis is approached in the future.					
32126781	2	19	from	applications	397:408	arg1	glycoproteomics					413:427	glycoproteomics	413:427	glycoproteomics	413:427	This includes MS/MS-based structural interrogation of glycopeptides for applications in glycoproteomics.					
32126781	4	20	theme	glycoprotein	711:722	arg1	glycoprofile					684:695	glycoprofile	684:695	glycoprofile	684:695	In turn, this allows inferences with respect to the glycoprofile of the intact glycoprotein.					
32126781	5	21	theme	group	842:846	arg1	topology					810:817	glycosidic topology	799:817	glycosidic topology of the oligosaccharide group	799:846	For glycopeptide analysis, CID is best known for the ability to determine glycosidic topology of the oligosaccharide group; however, CID has also been shown to produce amide bond cleavage of the polypeptide group.					
32126781	5	22	theme	polypeptide	920:930	arg1	group					932:936	the polypeptide group	916:936	the polypeptide group	916:936	For glycopeptide analysis, CID is best known for the ability to determine glycosidic topology of the oligosaccharide group; however, CID has also been shown to produce amide bond cleavage of the polypeptide group.					
32126781	10	23	theme	peptide	1667:1673	arg1	dissociation					1675:1686	peptide dissociation	1667:1686	peptide dissociation	1667:1686	Thus, deliberately obtaining either glycan or peptide dissociation is a more delicate undertaking for O-glycopeptides.					
32126781	3	24	theme	measurements	451:462	arg1	goal					438:441	The end goal	430:441	The end goal of such measurements	430:462	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	25	theme	end	434:436	arg1	goal					438:441	The end goal	430:441	The end goal of such measurements	430:462	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	26	theme	such	446:449	arg1	measurements					451:462	such measurements	446:462	such measurements	446:462	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	7	27	from	dearth	1205:1210	arg1	glycopeptides					1240:1252	O-linked glycopeptides	1231:1252	O-linked glycopeptides	1231:1252	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	11	28	theme	O-glycoproteomic	1854:1869	arg1	analysis					1871:1878	O-glycoproteomic analysis	1854:1878	O-glycoproteomic analysis	1854:1878	Establishing a more complete understanding of O-glycopeptide ER-CID is likely to have a substantive impact on how O-glycoproteomic analysis is approached in the future.					
32126781	7	29	link	O-linked	1231:1238	arg1	glycopeptides					1240:1252	O-linked glycopeptides	1231:1252	O-linked glycopeptides	1231:1252	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	3	30	theme	monosaccharide	484:497	arg1	connectivity					499:510	the monosaccharide connectivity	480:510	the monosaccharide connectivity of the glycan	480:524	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	0	31	theme	Parallel	0:7	arg1	Determination					9:21	Parallel Determination	0:21	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides	0:150	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides Derived from Nonspecific Proteolysis.					
32126781	9	32	theme	O-glycopeptides	1527:1541	arg1	CID					1511:1513	the energy-resolved CID	1491:1513	the energy-resolved CID (ER-CID) of O-glycopeptides	1491:1541	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	9	32	theme	O-glycopeptides	1527:1541	arg1	sensitive					1578:1586	sensitive	1578:1586	sensitive	1578:1586	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	9	32	theme	O-glycopeptides	1527:1541	arg1	ER-CID					1516:1521	ER-CID	1516:1521	ER-CID	1516:1521	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	10	33	dep	deliberately	1627:1638	arg1	obtaining					1640:1648	obtaining	1640:1648	obtaining either glycan or peptide dissociation	1640:1686	Thus, deliberately obtaining either glycan or peptide dissociation is a more delicate undertaking for O-glycopeptides.					
32126781	3	34	theme	interest	622:629	arg1	glycopeptide					606:617	each glycopeptide	601:617	each glycopeptide of interest	601:629	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	4	35	theme	intact	704:709	arg1	glycoprotein					711:722	the intact glycoprotein	700:722	the intact glycoprotein	700:722	In turn, this allows inferences with respect to the glycoprofile of the intact glycoprotein.					
32126781	0	36	theme	Polypeptide	26:36	arg1	Determination					9:21	Parallel Determination	0:21	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides	0:150	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides Derived from Nonspecific Proteolysis.					
32126781	8	37	theme	peptide	1317:1323	arg1	fragmentation					1334:1346	substantial peptide backbone fragmentation	1305:1346	substantial peptide backbone fragmentation	1305:1346	In this study, MS/MS via CID was shown to provide substantial peptide backbone fragmentation, in addition to glycosidic fragmentation, in an energy-dependent manner.					
32126781	7	38	theme	energy-resolved	1082:1096	arg1	pathways					1112:1119	these energy-resolved fragmentation pathways	1076:1119	these energy-resolved fragmentation pathways	1076:1119	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	7	38	theme	energy-resolved	1082:1096	arg1	subject					1135:1141	the subject	1131:1141	the subject of several studies on N-linked glycopeptides	1131:1186	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	2	39	theme	structural	351:360	arg1	interrogation					362:374	MS/MS-based structural interrogation	339:374	MS/MS-based structural interrogation of glycopeptides for applications in glycoproteomics	339:427	This includes MS/MS-based structural interrogation of glycopeptides for applications in glycoproteomics.					
32126781	9	40	theme	energy-resolved	1495:1509	arg1	CID					1511:1513	the energy-resolved CID	1491:1513	the energy-resolved CID (ER-CID) of O-glycopeptides	1491:1541	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	9	40	theme	energy-resolved	1495:1509	arg1	sensitive					1578:1586	sensitive	1578:1586	sensitive	1578:1586	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	9	40	theme	energy-resolved	1495:1509	arg1	ER-CID					1516:1521	ER-CID	1516:1521	ER-CID	1516:1521	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	9	41	theme	energy	1605:1610	arg1	setting					1612:1618	the collision energy setting	1591:1618	the collision energy setting	1591:1618	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	0	42	theme	Nonspecific	165:175	arg1	Proteolysis					177:187	Nonspecific Proteolysis	165:187	Nonspecific Proteolysis	165:187	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides Derived from Nonspecific Proteolysis.					
32126781	9	43	gly	N-glycopeptides	1474:1488	arg2	N-glycopeptides					1474:1488	N-glycopeptides	1474:1488	N-glycopeptides	1474:1488	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	2	44	theme	MS/MS-based	339:349	arg1	interrogation					362:374	MS/MS-based structural interrogation	339:374	MS/MS-based structural interrogation of glycopeptides for applications in glycoproteomics	339:427	This includes MS/MS-based structural interrogation of glycopeptides for applications in glycoproteomics.					
32126781	3	45	theme	amino	531:535	arg1	sequence					542:549	the amino acid sequence	527:549	the amino acid sequence of the peptide	527:564	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	0	46	theme	Oligosaccharide	42:56	arg1	Connectivities					58:71	Oligosaccharide Connectivities	42:71	Oligosaccharide Connectivities	42:71	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides Derived from Nonspecific Proteolysis.					
32126781	9	47	theme	collision	1595:1603	arg1	setting					1612:1618	the collision energy setting	1591:1618	the collision energy setting	1591:1618	While qualitatively similar to previous findings for N-glycopeptides, the energy-resolved CID (ER-CID) of O-glycopeptides was found to be substantially more sensitive to the collision energy setting.					
32126781	3	48	theme	glycan	519:524	arg1	connectivity					499:510	the monosaccharide connectivity	480:510	the monosaccharide connectivity of the glycan	480:524	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	48	theme	glycan	519:524	arg1	sequence					542:549	the amino acid sequence	527:549	the amino acid sequence of the peptide	527:564	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	48	theme	glycan	519:524	arg1	site					575:578	the site	571:578	the site of glycosylation for each glycopeptide of interest	571:629	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	48	theme	glycan	519:524	arg1	glycan					519:524	the glycan	515:524	the glycan	515:524	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	48	theme	glycan	519:524	arg1	glycosylation					583:595	glycosylation	583:595	glycosylation	583:595	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	48	theme	glycan	519:524	arg1	peptide					558:564	the peptide	554:564	the peptide	554:564	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	5	49	theme	bond	899:902	arg1	cleavage					904:911	amide bond cleavage	893:911	amide bond cleavage of the polypeptide group	893:936	For glycopeptide analysis, CID is best known for the ability to determine glycosidic topology of the oligosaccharide group; however, CID has also been shown to produce amide bond cleavage of the polypeptide group.					
32126781	1	50	theme	applied	254:260	arg1	method					275:280	the most broadly applied dissociation method	237:280	the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS)	237:322	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32126781	1	50	theme	applied	254:260	arg1	dissociation					208:219	Collision-induced dissociation	190:219	Collision-induced dissociation (CID)	190:225	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32126781	7	51	from	subject	1135:1141	arg1	glycopeptides					1174:1186	N-linked glycopeptides	1165:1186	N-linked glycopeptides	1165:1186	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	5	52	theme	amide	893:897	arg1	cleavage					904:911	amide bond cleavage	893:911	amide bond cleavage of the polypeptide group	893:936	For glycopeptide analysis, CID is best known for the ability to determine glycosidic topology of the oligosaccharide group; however, CID has also been shown to produce amide bond cleavage of the polypeptide group.					
32126781	8	53	theme	backbone	1325:1332	arg1	fragmentation					1334:1346	substantial peptide backbone fragmentation	1305:1346	substantial peptide backbone fragmentation	1305:1346	In this study, MS/MS via CID was shown to provide substantial peptide backbone fragmentation, in addition to glycosidic fragmentation, in an energy-dependent manner.					
32126781	7	54	link	N-linked	1165:1172	arg1	glycopeptides					1174:1186	N-linked glycopeptides	1165:1186	N-linked glycopeptides	1165:1186	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	1	55	theme	dissociation	262:273	arg1	method					275:280	the most broadly applied dissociation method	237:280	the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS)	237:322	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32126781	1	55	theme	dissociation	262:273	arg1	dissociation					208:219	Collision-induced dissociation	190:219	Collision-induced dissociation (CID)	190:225	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32126781	5	56	theme	oligosaccharide	826:840	arg1	group					842:846	the oligosaccharide group	822:846	the oligosaccharide group	822:846	For glycopeptide analysis, CID is best known for the ability to determine glycosidic topology of the oligosaccharide group; however, CID has also been shown to produce amide bond cleavage of the polypeptide group.					
32126781	7	57	theme	work	1223:1226	arg1	dearth					1205:1210	a dearth	1203:1210	a dearth of similar work on O-linked glycopeptides	1203:1252	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	5	58	theme	glycopeptide	729:740	arg1	analysis					742:749	glycopeptide analysis	729:749	glycopeptide analysis	729:749	For glycopeptide analysis, CID is best known for the ability to determine glycosidic topology of the oligosaccharide group; however, CID has also been shown to produce amide bond cleavage of the polypeptide group.					
32126781	5	59	theme	group	932:936	arg1	cleavage					904:911	amide bond cleavage	893:911	amide bond cleavage of the polypeptide group	893:936	For glycopeptide analysis, CID is best known for the ability to determine glycosidic topology of the oligosaccharide group; however, CID has also been shown to produce amide bond cleavage of the polypeptide group.					
32126781	0	60	theme	Connectivities	58:71	arg1	Determination					9:21	Parallel Determination	0:21	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides	0:150	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides Derived from Nonspecific Proteolysis.					
32126781	7	61	theme	similar	1215:1221	arg1	work					1223:1226	similar work	1215:1226	similar work	1215:1226	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	11	62	theme	substantive	1828:1838	arg1	impact					1840:1845	a substantive impact	1826:1845	a substantive impact	1826:1845	Establishing a more complete understanding of O-glycopeptide ER-CID is likely to have a substantive impact on how O-glycoproteomic analysis is approached in the future.					
32126781	7	63	from	studies	1154:1160	arg1	glycopeptides					1174:1186	N-linked glycopeptides	1165:1186	N-linked glycopeptides	1165:1186	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	2	64	gly	glycopeptides	379:391	arg2	glycopeptides					379:391	glycopeptides	379:391	glycopeptides	379:391	This includes MS/MS-based structural interrogation of glycopeptides for applications in glycoproteomics.					
32126781	11	65	theme	O-glycopeptide	1786:1799	arg1	ER-CID					1801:1806	O-glycopeptide ER-CID	1786:1806	O-glycopeptide ER-CID	1786:1806	Establishing a more complete understanding of O-glycopeptide ER-CID is likely to have a substantive impact on how O-glycoproteomic analysis is approached in the future.					
32126781	7	66	theme	N-linked	1165:1172	arg1	glycopeptides					1174:1186	N-linked glycopeptides	1165:1186	N-linked glycopeptides	1165:1186	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	7	67	gly	glycopeptides	1174:1186	arg2	glycopeptides					1174:1186	N-linked glycopeptides	1165:1186	N-linked glycopeptides	1165:1186	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	3	68	gly	glycopeptide	606:617	arg2	glycopeptide					606:617	each glycopeptide	601:617	each glycopeptide of interest	601:629	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	68	gly	glycopeptide	606:617	arg1	interest					622:629	interest	622:629	interest	622:629	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	7	69	gly	glycopeptides	1240:1252	arg2	glycopeptides					1240:1252	O-linked glycopeptides	1231:1252	O-linked glycopeptides	1231:1252	While these energy-resolved fragmentation pathways have been the subject of several studies on N-linked glycopeptides, there remains a dearth of similar work on O-linked glycopeptides.					
32126781	8	70	theme	glycosidic	1364:1373	arg1	fragmentation					1375:1387	glycosidic fragmentation	1364:1387	glycosidic fragmentation	1364:1387	In this study, MS/MS via CID was shown to provide substantial peptide backbone fragmentation, in addition to glycosidic fragmentation, in an energy-dependent manner.					
32126781	11	71	theme	complete	1760:1767	arg1	understanding					1769:1781	a more complete understanding	1753:1781	a more complete understanding of O-glycopeptide ER-CID	1753:1806	Establishing a more complete understanding of O-glycopeptide ER-CID is likely to have a substantive impact on how O-glycoproteomic analysis is approached in the future.					
32126781	2	72	theme	glycopeptides	379:391	arg1	interrogation					362:374	MS/MS-based structural interrogation	339:374	MS/MS-based structural interrogation of glycopeptides for applications in glycoproteomics	339:427	This includes MS/MS-based structural interrogation of glycopeptides for applications in glycoproteomics.					
32126781	3	73	theme	acid	537:540	arg1	sequence					542:549	the amino acid sequence	527:549	the amino acid sequence of the peptide	527:564	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	0	74	theme	Collison-Induced	92:107	arg1	Dissociation					109:120	Energy-Resolved Collison-Induced Dissociation	76:120	Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides	76:150	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides Derived from Nonspecific Proteolysis.					
32126781	6	75	theme	applied	1044:1050	arg1	energy					1062:1067	the applied collision energy	1040:1067	the applied collision energy	1040:1067	Whether structural information is obtained for the glycan or the peptide has been found to depend on the applied collision energy.					
32126781	8	76	theme	substantial	1305:1315	arg1	fragmentation					1334:1346	substantial peptide backbone fragmentation	1305:1346	substantial peptide backbone fragmentation	1305:1346	In this study, MS/MS via CID was shown to provide substantial peptide backbone fragmentation, in addition to glycosidic fragmentation, in an energy-dependent manner.					
32126781	11	77	theme	ER-CID	1801:1806	arg1	understanding					1769:1781	a more complete understanding	1753:1781	a more complete understanding of O-glycopeptide ER-CID	1753:1806	Establishing a more complete understanding of O-glycopeptide ER-CID is likely to have a substantive impact on how O-glycoproteomic analysis is approached in the future.					
32126781	0	78	theme	Energy-Resolved	76:90	arg1	Dissociation					109:120	Energy-Resolved Collison-Induced Dissociation	76:120	Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides	76:150	Parallel Determination of Polypeptide and Oligosaccharide Connectivities by Energy-Resolved Collison-Induced Dissociation of Protonated O-Glycopeptides Derived from Nonspecific Proteolysis.					
32126781	6	79	theme	structural	947:956	arg1	information					958:968	structural information	947:968	structural information	947:968	Whether structural information is obtained for the glycan or the peptide has been found to depend on the applied collision energy.					
32126781	3	80	gly	glycosylation	583:595	arg2	site					575:578	the site	571:578	the site of glycosylation for each glycopeptide of interest	571:629	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	80	gly	glycosylation	583:595	arg2	glycan					519:524	the glycan	515:524	the glycan	515:524	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	80	gly	glycosylation	583:595	arg2	peptide					558:564	the peptide	554:564	the peptide	554:564	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	3	80	gly	glycosylation	583:595	arg2	glycosylation					583:595	glycosylation	583:595	glycosylation	583:595	The end goal of such measurements is to determine the monosaccharide connectivity of the glycan, the amino acid sequence of the peptide, and the site of glycosylation for each glycopeptide of interest.					
32126781	10	81	gly	O-glycopeptides	1723:1737	arg2	O-glycopeptides					1723:1737	O-glycopeptides	1723:1737	O-glycopeptides	1723:1737	Thus, deliberately obtaining either glycan or peptide dissociation is a more delicate undertaking for O-glycopeptides.					
32126781	1	82	theme	tandem	291:296	arg1	spectrometry					303:314	tandem mass spectrometry	291:314	tandem mass spectrometry (MS/MS)	291:322	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32126781	1	82	theme	tandem	291:296	arg1	MS/MS					317:321	MS/MS	317:321	MS/MS	317:321	Collision-induced dissociation (CID) is by far the most broadly applied dissociation method used for tandem mass spectrometry (MS/MS).					
32426967	4	0	theme	glycoforms	922:931	arg1	quantification					890:903	quantification	890:903	quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	890:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	7	1	theme	HCC	1492:1494	arg1	patients					1496:1503	cirrhosis and HCC patients	1478:1503	patients	1496:1503	We found that our strategy was able to distinguish isomers of glycopeptides where several isomers showed distinctly different patterns between cirrhosis and HCC patients.					
32426967	3	2	with	immunoprecipitation	537:555	arg1	beads					600:604	anti-A1AT antibody conjugated agarose beads	562:604	anti-A1AT antibody conjugated agarose beads	562:604	Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS.					
32426967	3	3	theme	conjugated	581:590	arg1	beads					600:604	anti-A1AT antibody conjugated agarose beads	562:604	anti-A1AT antibody conjugated agarose beads	562:604	Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS.					
32426967	7	4	theme	cirrhosis	1478:1486	arg1	patients					1496:1503	cirrhosis and HCC patients	1478:1503	patients	1496:1503	We found that our strategy was able to distinguish isomers of glycopeptides where several isomers showed distinctly different patterns between cirrhosis and HCC patients.					
32426967	4	5	theme	mass	687:690	arg1	strategies					705:714	Two tandem mass spectrometry strategies	676:714	Two tandem mass spectrometry strategies	676:714	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	3	6	theme	anti-A1AT	562:570	arg1	beads					600:604	anti-A1AT antibody conjugated agarose beads	562:604	anti-A1AT antibody conjugated agarose beads	562:604	Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS.					
32426967	7	7	gly	glycopeptides	1397:1409	arg2	glycopeptides					1397:1409	glycopeptides	1397:1409	glycopeptides	1397:1409	We found that our strategy was able to distinguish isomers of glycopeptides where several isomers showed distinctly different patterns between cirrhosis and HCC patients.					
32426967	4	8	gly	glycoforms	922:931	arg1	A1AT					936:939	A1AT	936:939	A1AT in HCC and cirrhosis patient sera	936:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	2	9	theme	spectrometry-based	295:312	arg1	method					314:319	a liquid chromatography-tandem mass spectrometry-based method	259:319	a liquid chromatography-tandem mass spectrometry-based method	259:319	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	4	10	from	cirrhosis	952:960	arg1	glycoforms					922:931	site-specific glycoforms	908:931	site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	908:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	4	11	theme	A1AT	936:939	arg1	glycoforms					922:931	site-specific glycoforms	908:931	site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	908:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	0	12	from	Patients	71:78	arg1	Analysis					13:20	Quantitative Analysis	0:20	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients	0:78	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS.					
32426967	4	13	from	HCC	944:946	arg1	glycoforms					922:931	site-specific glycoforms	908:931	site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	908:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	3	14	theme	isolated	615:622	arg1	protein					629:635	the isolated A1AT protein	611:635	the isolated A1AT protein	611:635	Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS.					
32426967	1	15	theme	serum	132:136	arg1	proteins					138:145	serum proteins	132:145	serum proteins	132:145	The change in glycosylation of serum proteins is often associated with the development of various diseases and thus can be used for diagnosis.					
32426967	4	16	theme	nontargeted	748:758	arg1	strategy					772:779	a nontargeted stepped HCD strategy	746:779	a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	746:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	4	17	from	A1AT	936:939	arg1	cirrhosis					952:960	cirrhosis	952:960	cirrhosis	952:960	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	4	17	from	A1AT	936:939	arg1	HCC					944:946	HCC	944:946	HCC	944:946	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	6	18	theme	patient	1243:1249	arg1	samples					1251:1257	patient samples	1243:1257	patient samples	1243:1257	Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.					
32426967	8	19	dep	curve	1718:1722	arg1	AUC					1724:1726	AUC	1724:1726	characteristic curve AUC of 0.9	1703:1733	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	4	20	from	glycoforms	922:931	arg1	cirrhosis					952:960	cirrhosis	952:960	cirrhosis	952:960	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	4	20	from	glycoforms	922:931	arg1	HCC					944:946	HCC	944:946	HCC	944:946	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	9	21	theme	sialic	1802:1807	arg1	linkage					1824:1830	the sialic acid/galactose linkage	1798:1830	the sialic acid/galactose linkage of the glycan motif	1798:1850	Further analysis showed that the difference may be related to the sialic acid/galactose linkage of the glycan motif.					
32426967	8	22	theme	different	1538:1546	arg1	states					1555:1560	different charge states	1538:1560	different charge states (2+/3+) of one glycopeptide of A1AT	1538:1596	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	8	22	theme	different	1538:1546	arg1	2+/3+					1563:1567	2+/3+	1563:1567	2+/3+	1563:1567	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	7	23	theme	glycopeptides	1397:1409	arg1	isomers					1386:1392	isomers	1386:1392	isomers of glycopeptides	1386:1409	We found that our strategy was able to distinguish isomers of glycopeptides where several isomers showed distinctly different patterns between cirrhosis and HCC patients.					
32426967	2	24	from	patients	474:481	arg1	glycoforms					398:407	site-specific glycoforms	384:407	site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients	384:481	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	5	25	used	used	1012:1015	arg2	software					999:1006	pGlyco2.0 software	989:1006	pGlyco2.0 software	989:1006	Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra.					
32426967	4	26	theme	A1AT	808:811	arg1	glycopeptides					813:825	A1AT glycopeptides	808:825	A1AT glycopeptides	808:825	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	2	27	theme	α-1-antitrypsin	418:432	arg1	glycoforms					398:407	site-specific glycoforms	384:407	site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients	384:481	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	0	28	theme	Isoforms	55:62	arg1	Analysis					13:20	Quantitative Analysis	0:20	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients	0:78	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS.					
32426967	2	29	theme	cirrhosis	464:472	arg1	patients					474:481	early-stage HCC and cirrhosis patients	444:481	patients	474:481	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	2	30	gly	glycoforms	398:407	arg1	A1AT					435:438	A1AT	435:438	A1AT	435:438	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	2	30	gly	glycoforms	398:407	arg1	α-1-antitrypsin					418:432	serum α-1-antitrypsin	412:432	serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients	412:481	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	4	31	theme	HCD	768:770	arg1	strategy					772:779	a nontargeted stepped HCD strategy	746:779	a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	746:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	0	32	theme	Quantitative	0:11	arg1	Analysis					13:20	Quantitative Analysis	0:20	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients	0:78	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS.					
32426967	8	33	theme	glycopeptide	1577:1588	arg1	states					1555:1560	different charge states	1538:1560	different charge states (2+/3+) of one glycopeptide of A1AT	1538:1596	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	8	33	theme	glycopeptide	1577:1588	arg1	2+/3+					1563:1567	2+/3+	1563:1567	2+/3+	1563:1567	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	3	34	theme	Serum	484:488	arg1	A1AT					498:501	Serum protein A1AT	484:501	Serum protein A1AT	484:501	Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS.					
32426967	8	35	theme	A1AT	1593:1596	arg1	glycopeptide					1577:1588	one glycopeptide	1573:1588	one glycopeptide of A1AT	1573:1596	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	9	36	theme	Further	1736:1742	arg1	analysis					1744:1751	Further analysis	1736:1751	Further analysis	1736:1751	Further analysis showed that the difference may be related to the sialic acid/galactose linkage of the glycan motif.					
32426967	4	37	theme	reaction	851:858	arg1	PRM					872:874	PRM	872:874	PRM	872:874	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	4	37	theme	reaction	851:858	arg1	monitoring					860:869	parallel reaction monitoring	842:869	a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	831:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	0	38	theme	Glycosylation	41:53	arg1	Isoforms					55:62	α-1-Antitrypsin Glycosylation Isoforms	25:62	α-1-Antitrypsin Glycosylation Isoforms in HCC Patients	25:78	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS.					
32426967	8	39	theme	charge	1548:1553	arg1	states					1555:1560	different charge states	1538:1560	different charge states (2+/3+) of one glycopeptide of A1AT	1538:1596	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	8	39	theme	charge	1548:1553	arg1	2+/3+					1563:1567	2+/3+	1563:1567	2+/3+	1563:1567	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	2	40	from	α-1-antitrypsin	418:432	arg1	HCC					456:458	early-stage HCC and cirrhosis patients	444:481	HCC	456:458	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	2	40	from	α-1-antitrypsin	418:432	arg1	patients					474:481	early-stage HCC and cirrhosis patients	444:481	patients	474:481	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	8	41	theme	early-stage	1629:1639	arg1	HCC					1641:1643	early-stage HCC	1629:1643	early-stage HCC from cirrhosis	1629:1658	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	2	42	theme	accurate	333:340	arg1	analysis					353:360	accurate structural analysis	333:360	accurate structural analysis	333:360	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	4	43	theme	targeted	833:840	arg1	strategy					877:884	a targeted parallel reaction monitoring (PRM) strategy	831:884	a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	831:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	5	44	used	used	1075:1078	arg2	software					1062:1069	Skyline software	1054:1069	Skyline software	1054:1069	Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra.					
32426967	1	45	from	change	105:110	arg1	glycosylation					115:127	glycosylation	115:127	glycosylation of serum proteins	115:145	The change in glycosylation of serum proteins is often associated with the development of various diseases and thus can be used for diagnosis.					
32426967	3	46	theme	patient	521:527	arg1	sera					529:532	patient sera	521:532	patient sera	521:532	Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS.					
32426967	6	47	theme	patient	1318:1324	arg1	samples					1326:1332	patient samples	1318:1332	patient samples	1318:1332	Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.					
32426967	3	48	theme	antibody	572:579	arg1	beads					600:604	anti-A1AT antibody conjugated agarose beads	562:604	anti-A1AT antibody conjugated agarose beads	562:604	Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS.					
32426967	4	49	theme	site-specific	908:920	arg1	glycoforms					922:931	site-specific glycoforms	908:931	site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	908:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	6	50	theme	stepped	1222:1228	arg1	HCD-MS/MS					1230:1238	stepped HCD-MS/MS	1222:1238	stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples	1222:1332	Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.					
32426967	2	51	theme	liquid	261:266	arg1	method					314:319	a liquid chromatography-tandem mass spectrometry-based method	259:319	a liquid chromatography-tandem mass spectrometry-based method	259:319	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	4	52	theme	tandem	680:685	arg1	strategies					705:714	Two tandem mass spectrometry strategies	676:714	Two tandem mass spectrometry strategies	676:714	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	1	53	theme	various	191:197	arg1	diseases					199:206	various diseases	191:206	various diseases	191:206	The change in glycosylation of serum proteins is often associated with the development of various diseases and thus can be used for diagnosis.					
32426967	4	54	theme	strategy	877:884	arg1	analysis					796:803	structural analysis	785:803	structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	785:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	6	55	theme	A1AT	1196:1199	arg1	glycopeptides					1179:1191	Ten site-specific glycopeptides	1161:1191	Ten site-specific glycopeptides of A1AT	1161:1199	Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.					
32426967	4	56	theme	spectrometry	692:703	arg1	strategies					705:714	Two tandem mass spectrometry strategies	676:714	Two tandem mass spectrometry strategies	676:714	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	6	57	gly	glycopeptides	1179:1191	arg1	A1AT					1196:1199	A1AT	1196:1199	A1AT	1196:1199	Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.					
32426967	6	57	gly	glycopeptides	1179:1191	arg2	glycopeptides					1179:1191	Ten site-specific glycopeptides	1161:1191	Ten site-specific glycopeptides of A1AT	1161:1199	Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.					
32426967	3	58	theme	agarose	592:598	arg1	beads					600:604	anti-A1AT antibody conjugated agarose beads	562:604	anti-A1AT antibody conjugated agarose beads	562:604	Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS.					
32426967	2	59	theme	site-specific	384:396	arg1	glycoforms					398:407	site-specific glycoforms	384:407	site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients	384:481	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	7	60	theme	different	1451:1459	arg1	patterns					1461:1468	distinctly different patterns	1440:1468	distinctly different patterns between cirrhosis and HCC patients	1440:1503	We found that our strategy was able to distinguish isomers of glycopeptides where several isomers showed distinctly different patterns between cirrhosis and HCC patients.					
32426967	8	61	from	cirrhosis	1650:1658	arg1	HCC					1641:1643	early-stage HCC	1629:1643	early-stage HCC from cirrhosis	1629:1658	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	5	62	theme	Y1	1119:1120	arg1	peptide+GlcNAc					1127:1140	peptide+GlcNAc	1127:1140	peptide+GlcNAc	1127:1140	Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra.					
32426967	5	62	theme	Y1	1119:1120	arg1	ion					1122:1124	the Y1 ion	1115:1124	the Y1 ion (peptide+GlcNAc) in MS/MS spectra	1115:1158	Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra.					
32426967	3	63	theme	A1AT	624:627	arg1	protein					629:635	the isolated A1AT protein	611:635	the isolated A1AT protein	611:635	Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS.					
32426967	1	64	theme	diseases	199:206	arg1	development					176:186	the development	172:186	the development of various diseases	172:206	The change in glycosylation of serum proteins is often associated with the development of various diseases and thus can be used for diagnosis.					
32426967	4	65	theme	patient	962:968	arg1	sera					970:973	patient sera	962:973	patient sera	962:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	8	66	theme	states	1555:1560	arg1	ratio					1529:1533	the ratio	1525:1533	the ratio of different charge states (2+/3+) of one glycopeptide of A1AT	1525:1596	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	7	67	theme	several	1417:1423	arg1	isomers					1425:1431	several isomers	1417:1431	several isomers	1417:1431	We found that our strategy was able to distinguish isomers of glycopeptides where several isomers showed distinctly different patterns between cirrhosis and HCC patients.					
32426967	5	68	theme	glycoform	1084:1092	arg1	quantification					1094:1107	glycoform quantification	1084:1107	glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra	1084:1158	Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra.					
32426967	1	69	theme	proteins	138:145	arg1	glycosylation					115:127	glycosylation	115:127	glycosylation of serum proteins	115:145	The change in glycosylation of serum proteins is often associated with the development of various diseases and thus can be used for diagnosis.					
32426967	6	70	theme	samples	1251:1257	arg1	samples					1251:1257	patient samples	1243:1257	patient samples	1243:1257	Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.					
32426967	6	70	theme	samples	1251:1257	arg1	7					1260:1260	7	1260:1260	7	1260:1260	Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.					
32426967	4	71	dep	HCC	944:946	arg1	sera					970:973	patient sera	962:973	patient sera	962:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	2	72	from	HCC	456:458	arg1	glycoforms					398:407	site-specific glycoforms	384:407	site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients	384:481	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	9	73	theme	acid/galactose	1809:1822	arg1	linkage					1824:1830	the sialic acid/galactose linkage	1798:1830	the sialic acid/galactose linkage of the glycan motif	1798:1850	Further analysis showed that the difference may be related to the sialic acid/galactose linkage of the glycan motif.					
32426967	0	74	from	Analysis	13:20	arg1	Patients					71:78	HCC Patients	67:78	HCC Patients	67:78	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS.					
32426967	5	75	from	ion	1122:1124	arg1	spectra					1152:1158	MS/MS spectra	1146:1158	MS/MS spectra	1146:1158	Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra.					
32426967	8	76	gly	glycopeptide	1577:1588	arg1	A1AT					1593:1596	A1AT	1593:1596	A1AT	1593:1596	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	8	76	gly	glycopeptide	1577:1588	arg2	glycopeptide					1577:1588	one glycopeptide	1573:1588	one glycopeptide of A1AT	1573:1596	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	4	77	theme	structural	785:794	arg1	analysis					796:803	structural analysis	785:803	structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	785:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	1	78	gly	glycosylation	115:127	arg1	proteins					138:145	serum proteins	132:145	serum proteins	132:145	The change in glycosylation of serum proteins is often associated with the development of various diseases and thus can be used for diagnosis.					
32426967	9	79	theme	glycan	1839:1844	arg1	motif					1846:1850	the glycan motif	1835:1850	the glycan motif	1835:1850	Further analysis showed that the difference may be related to the sialic acid/galactose linkage of the glycan motif.					
32426967	0	80	from	Isoforms	55:62	arg1	Patients					71:78	HCC Patients	67:78	HCC Patients	67:78	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS.					
32426967	6	81	from	HCD-MS/MS	1230:1238	arg1	samples					1251:1257	patient samples	1243:1257	patient samples	1243:1257	Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.					
32426967	2	82	theme	serum	412:416	arg1	A1AT					435:438	A1AT	435:438	A1AT	435:438	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	2	82	theme	serum	412:416	arg1	α-1-antitrypsin					418:432	serum α-1-antitrypsin	412:432	serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients	412:481	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	5	83	theme	MS/MS	1146:1150	arg1	spectra					1152:1158	MS/MS spectra	1146:1158	MS/MS spectra	1146:1158	Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra.					
32426967	4	84	theme	stepped	760:766	arg1	strategy					772:779	a nontargeted stepped HCD strategy	746:779	a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	746:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	2	85	theme	glycoforms	398:407	arg1	quantification					366:379	quantification	366:379	quantification	366:379	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	2	85	theme	glycoforms	398:407	arg1	analysis					353:360	accurate structural analysis	333:360	accurate structural analysis	333:360	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	0	86	theme	α-1-Antitrypsin	25:39	arg1	Isoforms					55:62	α-1-Antitrypsin Glycosylation Isoforms	25:62	α-1-Antitrypsin Glycosylation Isoforms in HCC Patients	25:78	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS.					
32426967	2	87	theme	early-stage	444:454	arg1	HCC					456:458	early-stage HCC and cirrhosis patients	444:481	HCC	456:458	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	3	88	theme	protein	490:496	arg1	A1AT					498:501	Serum protein A1AT	484:501	Serum protein A1AT	484:501	Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS.					
32426967	4	89	gly	glycopeptides	813:825	arg2	glycopeptides					813:825	A1AT glycopeptides	808:825	A1AT glycopeptides	808:825	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	5	90	theme	Skyline	1054:1060	arg1	software					1062:1069	Skyline software	1054:1069	Skyline software	1054:1069	Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra.					
32426967	5	91	gly	glycopeptide	1021:1032	arg2	glycopeptide					1021:1032	glycopeptide identification	1021:1047	glycopeptide identification	1021:1047	Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra.					
32426967	4	92	theme	parallel	842:849	arg1	PRM					872:874	PRM	872:874	PRM	872:874	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	4	92	theme	parallel	842:849	arg1	monitoring					860:869	parallel reaction monitoring	842:869	a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	831:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	0	93	theme	HCC	67:69	arg1	Patients					71:78	HCC Patients	67:78	HCC Patients	67:78	Quantitative Analysis of α-1-Antitrypsin Glycosylation Isoforms in HCC Patients Using LC-HCD-PRM-MS.					
32426967	2	94	from	glycoforms	398:407	arg1	HCC					456:458	early-stage HCC and cirrhosis patients	444:481	HCC	456:458	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	2	94	from	glycoforms	398:407	arg1	patients					474:481	early-stage HCC and cirrhosis patients	444:481	patients	474:481	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	6	95	theme	site-specific	1165:1177	arg1	glycopeptides					1179:1191	Ten site-specific glycopeptides	1161:1191	Ten site-specific glycopeptides of A1AT	1161:1199	Ten site-specific glycopeptides of A1AT were identified with stepped HCD-MS/MS in patient samples, 7 of which were further quantified using HCD-PRM-MS among patient samples.					
32426967	4	96	theme	monitoring	860:869	arg1	strategy					877:884	a targeted parallel reaction monitoring (PRM) strategy	831:884	a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	831:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	2	97	theme	structural	342:351	arg1	analysis					353:360	accurate structural analysis	333:360	accurate structural analysis	333:360	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	8	98	theme	characteristic	1703:1716	arg1	curve					1718:1722	characteristic curve AUC of 0.9	1703:1733	characteristic curve AUC of 0.9	1703:1733	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
32426967	5	99	theme	glycopeptide	1021:1032	arg1	identification					1034:1047	glycopeptide identification	1021:1047	glycopeptide identification	1021:1047	Accordingly, pGlyco2.0 software was used for glycopeptide identification, and Skyline software was used for glycoform quantification using the Y1 ion (peptide+GlcNAc) in MS/MS spectra.					
32426967	4	100	theme	glycopeptides	813:825	arg1	analysis					796:803	structural analysis	785:803	structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera	785:973	Two tandem mass spectrometry strategies are integrated in this study: a nontargeted stepped HCD strategy for structural analysis of A1AT glycopeptides and a targeted parallel reaction monitoring (PRM) strategy for quantification of site-specific glycoforms of A1AT in HCC and cirrhosis patient sera.					
32426967	1	101	used	used	224:227	arg2	change					105:110	The change	101:110	The change in glycosylation of serum proteins	101:145	The change in glycosylation of serum proteins is often associated with the development of various diseases and thus can be used for diagnosis.					
32426967	2	102	used	used	324:327	arg2	method					314:319	a liquid chromatography-tandem mass spectrometry-based method	259:319	a liquid chromatography-tandem mass spectrometry-based method	259:319	In this study, a liquid chromatography-tandem mass spectrometry-based method is used for accurate structural analysis and quantification of site-specific glycoforms of serum α-1-antitrypsin (A1AT) in early-stage HCC and cirrhosis patients.					
32426967	9	103	theme	motif	1846:1850	arg1	linkage					1824:1830	the sialic acid/galactose linkage	1798:1830	the sialic acid/galactose linkage of the glycan motif	1798:1850	Further analysis showed that the difference may be related to the sialic acid/galactose linkage of the glycan motif.					
32426967	8	104	theme	0.9	1731:1733	arg1	AUC					1724:1726	AUC	1724:1726	characteristic curve AUC of 0.9	1703:1733	We also found that the ratio of different charge states (2+/3+) of one glycopeptide of A1AT can significantly discriminate early-stage HCC from cirrhosis with the area under the receiver operating characteristic curve AUC of 0.9.					
33423189	0	0	theme	immunoglobulin	61:74	arg1	glycoprotein					80:91	mouse immunoglobulin G2b glycoprotein	55:91	mouse immunoglobulin G2b glycoprotein	55:91	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.					
33423189	6	1	theme	glycan-protein	1110:1123	arg1	interactions					1125:1136	glycan-protein interactions	1110:1136	glycan-protein interactions	1110:1136	The results indicated that degalactosylation structurally perturbed the Fc region through rearrangement of glycan-protein interactions.					
33423189	2	2	theme	domain	336:341	arg1	Asn297					317:322	Asn297	317:322	Asn297	317:322	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	0	3	theme	mouse	55:59	arg1	glycoprotein					80:91	mouse immunoglobulin G2b glycoprotein	55:91	mouse immunoglobulin G2b glycoprotein	55:91	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.					
33423189	2	4	from	Asn297	317:322	arg1	N-glycosylation					298:312	N-glycosylation	298:312	N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues	298:512	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	2	5	theme	CH2	332:334	arg1	domain					336:341	each CH2 domain	327:341	each CH2 domain	327:341	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	0	6	theme	glycoprotein	80:91	arg1	fragment					43:50	the Fc fragment	36:50	the Fc fragment of mouse immunoglobulin G2b glycoprotein	36:91	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.					
33423189	7	7	theme	glycoprotein	1176:1187	arg1	assignments					1152:1162	The spectral assignments	1139:1162	The spectral assignments of IgG2b-Fc glycoprotein	1139:1187	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	7	8	with	conformations	1249:1261	arg1	molecules					1294:1302	effector molecules	1285:1302	effector molecules in solution	1285:1314	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	4	9	theme	54 kDa	793:798	arg1	mass					785:788	a molecular mass	773:788	a molecular mass of 54 kDa	773:798	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	4	10	theme	molecular	775:783	arg1	mass					785:788	a molecular mass	773:788	a molecular mass of 54 kDa	773:798	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	1	11	theme	complement	232:241	arg1	C1q					253:255	complement component C1q	232:255	complement component C1q	232:255	The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q.					
33423189	0	12	theme	G2b	76:78	arg1	glycoprotein					80:91	mouse immunoglobulin G2b glycoprotein	55:91	mouse immunoglobulin G2b glycoprotein	55:91	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.					
33423189	1	13	theme	component	243:251	arg1	C1q					253:255	complement component C1q	232:255	complement component C1q	232:255	The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q.					
33423189	4	14	theme	structural	711:720	arg1	characterization					722:737	in-solution structural characterization	699:737	in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa	699:798	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	3	15	theme	Crystal	515:521	arg1	structures					523:532	Crystal structures	515:532	Crystal structures of Fc	515:538	Crystal structures of Fc have shown that a pair of N-glycans is located between the two CH2 domains.					
33423189	2	16	dep	presence	450:457	arg1	the					446:448	the	446:448	the	446:448	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	5	17	theme	polypeptide	859:869	arg1	backbones					871:879	polypeptide backbones	859:879	polypeptide backbones of Fc	859:885	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	2	18	theme	complex-type	362:373	arg1	oligosaccharides					375:390	biantennary complex-type oligosaccharides	350:390	biantennary complex-type oligosaccharides	350:390	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	5	19	theme	Fc	884:885	arg1	assignments					819:829	spectral assignments	810:829	spectral assignments of the N-glycans	810:846	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	5	19	theme	Fc	884:885	arg1	backbones					871:879	polypeptide backbones	859:879	polypeptide backbones of Fc	859:885	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	7	20	theme	NMR	1216:1218	arg1	investigation					1220:1232	NMR investigation	1216:1232	NMR investigation of its dynamic conformations and interactions with effector molecules in solution	1216:1314	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	5	21	theme	Fc	930:931	arg1	perturbations					913:925	conformational perturbations	898:925	conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation	898:1000	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	5	22	theme	N-glycans	838:846	arg1	assignments					819:829	spectral assignments	810:829	spectral assignments of the N-glycans	810:846	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	5	22	theme	N-glycans	838:846	arg1	backbones					871:879	polypeptide backbones	859:879	polypeptide backbones of Fc	859:885	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	7	23	theme	IgG2b-Fc	1167:1174	arg1	glycoprotein					1176:1187	IgG2b-Fc glycoprotein	1167:1187	IgG2b-Fc glycoprotein	1167:1187	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	1	24	theme	defensive	144:152	arg1	functions					163:171	defensive effector functions	144:171	defensive effector functions	144:171	The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q.					
33423189	2	25	theme	residues	505:512	arg1	absence					462:468	absence	462:468	absence	462:468	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	2	25	theme	residues	505:512	arg1	presence					450:457	presence	450:457	presence	450:457	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	7	26	with	interactions	1267:1278	arg1	molecules					1294:1302	effector molecules	1285:1302	effector molecules in solution	1285:1314	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	1	27	theme	effector	154:161	arg1	functions					163:171	defensive effector functions	144:171	defensive effector functions	144:171	The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q.					
33423189	0	28	theme	NMR	0:2	arg1	assignments					4:14	NMR assignments	0:14	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.	0:92	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.					
33423189	4	29	theme	IgG2b-Fc	748:755	arg1	glycoforms					757:766	mouse IgG2b-Fc glycoforms	742:766	mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa	742:798	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	0	30	gly	glycoprotein	80:91	arg1	glycoprotein					80:91	mouse immunoglobulin G2b glycoprotein	55:91	mouse immunoglobulin G2b glycoprotein	55:91	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.					
33423189	2	31	theme	biantennary	350:360	arg1	oligosaccharides					375:390	biantennary complex-type oligosaccharides	350:390	biantennary complex-type oligosaccharides	350:390	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	7	32	theme	dynamic	1241:1247	arg1	conformations					1249:1261	its dynamic conformations	1237:1261	its dynamic conformations	1237:1261	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	7	33	theme	spectral	1143:1150	arg1	assignments					1152:1162	The spectral assignments	1139:1162	The spectral assignments of IgG2b-Fc glycoprotein	1139:1187	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	3	34	theme	N-glycans	566:574	arg1	pair					558:561	a pair	556:561	a pair of N-glycans	556:574	Crystal structures of Fc have shown that a pair of N-glycans is located between the two CH2 domains.					
33423189	3	34	theme	N-glycans	566:574	arg1	located					579:585	located	579:585	located	579:585	Crystal structures of Fc have shown that a pair of N-glycans is located between the two CH2 domains.					
33423189	4	35	theme	metabolic	636:644	arg1	technique					663:671	our metabolic isotope labeling technique	632:671	our metabolic isotope labeling technique	632:671	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	7	36	theme	conformations	1249:1261	arg1	investigation					1220:1232	NMR investigation	1216:1232	NMR investigation of its dynamic conformations and interactions with effector molecules in solution	1216:1314	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	3	37	theme	Fc	537:538	arg1	structures					523:532	Crystal structures	515:532	Crystal structures of Fc	515:538	Crystal structures of Fc have shown that a pair of N-glycans is located between the two CH2 domains.					
33423189	2	38	theme	non-reducing	473:484	arg1	residues					505:512	non-reducing terminal galactose residues	473:512	non-reducing terminal galactose residues	473:512	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	4	39	theme	in-solution	699:709	arg1	characterization					722:737	in-solution structural characterization	699:737	in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa	699:798	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	2	40	theme	galactose	495:503	arg1	residues					505:512	non-reducing terminal galactose residues	473:512	non-reducing terminal galactose residues	473:512	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	0	41	theme	fragment	43:50	arg1	N-glycans					23:31	the N-glycans	19:31	the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein	19:91	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.					
33423189	4	42	theme	labeling	654:661	arg1	technique					663:671	our metabolic isotope labeling technique	632:671	our metabolic isotope labeling technique	632:671	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	3	43	theme	CH2	603:605	arg1	domains					607:613	the two CH2 domains	595:613	the two CH2 domains	595:613	Crystal structures of Fc have shown that a pair of N-glycans is located between the two CH2 domains.					
33423189	4	44	theme	mammalian	679:687	arg1	cells					689:693	mammalian cells	679:693	mammalian cells	679:693	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	1	45	theme	immune	180:185	arg1	system					187:192	the immune system	176:192	the immune system	176:192	The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q.					
33423189	0	46	theme	N-glycans	23:31	arg1	assignments					4:14	NMR assignments	0:14	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.	0:92	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.					
33423189	2	47	theme	terminal	486:493	arg1	residues					505:512	non-reducing terminal galactose residues	473:512	non-reducing terminal galactose residues	473:512	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	7	48	from	molecules	1294:1302	arg1	solution					1307:1314	solution	1307:1314	solution	1307:1314	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	4	49	theme	isotope	646:652	arg1	technique					663:671	our metabolic isotope labeling technique	632:671	our metabolic isotope labeling technique	632:671	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	0	50	theme	Fc	40:41	arg1	fragment					43:50	the Fc fragment	36:50	the Fc fragment of mouse immunoglobulin G2b glycoprotein	36:91	NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein.					
33423189	5	51	theme	conformational	898:911	arg1	perturbations					913:925	conformational perturbations	898:925	conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation	898:1000	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	2	52	contain	contain	392:398	arg1	oligosaccharides					375:390	biantennary complex-type oligosaccharides	350:390	biantennary complex-type oligosaccharides	350:390	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	2	52	contain	contain	392:398	arg2	microheterogeneities					400:419	microheterogeneities	400:419	microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues	400:512	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	1	53	theme	Fc	98:99	arg1	portion					101:107	The Fc portion	94:107	The Fc portion of immunoglobulin G (IgG)	94:133	The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q.					
33423189	4	54	gly	glycoforms	757:766	arg1	mouse					742:746	mouse IgG2b-Fc glycoforms	742:766	mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa	742:798	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	4	54	gly	glycoforms	757:766	arg1	IgG2b-Fc					748:755	mouse IgG2b-Fc glycoforms	742:766	mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa	742:798	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	7	55	theme	effector	1285:1292	arg1	molecules					1294:1302	effector molecules	1285:1302	effector molecules in solution	1285:1314	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	4	56	theme	mouse	742:746	arg1	glycoforms					757:766	mouse IgG2b-Fc glycoforms	742:766	mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa	742:798	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	4	57	theme	glycoforms	757:766	arg1	characterization					722:737	in-solution structural characterization	699:737	in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa	699:798	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	5	58	theme	enzymatic	974:982	arg1	degalactosylation					984:1000	enzymatic degalactosylation	974:1000	enzymatic degalactosylation	974:1000	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	5	58	theme	enzymatic	974:982	arg1	trimming					953:960	N-glycan trimming	944:960	N-glycan trimming	944:960	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	1	59	theme	immunoglobulin	112:125	arg1	IgG					130:132	IgG	130:132	IgG	130:132	The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q.					
33423189	1	59	theme	immunoglobulin	112:125	arg1	G					127:127	immunoglobulin G	112:127	immunoglobulin G (IgG)	112:133	The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q.					
33423189	5	60	theme	N-glycan	944:951	arg1	degalactosylation					984:1000	enzymatic degalactosylation	974:1000	enzymatic degalactosylation	974:1000	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	5	60	theme	N-glycan	944:951	arg1	trimming					953:960	N-glycan trimming	944:960	N-glycan trimming	944:960	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	6	61	theme	interactions	1125:1136	arg1	rearrangement					1093:1105	rearrangement	1093:1105	rearrangement of glycan-protein interactions	1093:1136	The results indicated that degalactosylation structurally perturbed the Fc region through rearrangement of glycan-protein interactions.					
33423189	1	62	theme	G	127:127	arg1	portion					101:107	The Fc portion	94:107	The Fc portion of immunoglobulin G (IgG)	94:133	The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q.					
33423189	7	63	gly	glycoprotein	1176:1187	arg1	glycoprotein					1176:1187	IgG2b-Fc glycoprotein	1167:1187	IgG2b-Fc glycoprotein	1167:1187	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	6	64	theme	Fc	1075:1076	arg1	region					1078:1083	the Fc region	1071:1083	the Fc region	1071:1083	The results indicated that degalactosylation structurally perturbed the Fc region through rearrangement of glycan-protein interactions.					
33423189	2	65	gly	N-glycosylation	298:312	arg1	Asn297					317:322	Asn297	317:322	Asn297	317:322	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	2	65	gly	N-glycosylation	298:312	arg2	Asn297					317:322	Asn297	317:322	Asn297	317:322	These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues.					
33423189	4	66	with	glycoforms	757:766	arg1	mass					785:788	a molecular mass	773:788	a molecular mass of 54 kDa	773:798	Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa.					
33423189	5	67	theme	spectral	810:817	arg1	assignments					819:829	spectral assignments	810:829	spectral assignments of the N-glycans	810:846	Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation.					
33423189	7	68	theme	interactions	1267:1278	arg1	investigation					1220:1232	NMR investigation	1216:1232	NMR investigation of its dynamic conformations and interactions with effector molecules in solution	1216:1314	The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.					
33423189	1	69	theme	Fcγ	214:216	arg1	receptors					218:226	Fcγ receptors	214:226	Fcγ receptors	214:226	The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q.					
32141465	0	0	theme	-glucans	69:76	arg1	synthesis					35:43	stereoselective synthesis	19:43	stereoselective synthesis of teichoic acid α-(1,2)-glucans	19:76	Reagent controlled stereoselective synthesis of teichoic acid α-(1,2)-glucans.					
32141465	3	1	theme	α-1,2-glucans	541:553	arg1	construction					525:536	the construction	521:536	the construction of α-1,2-glucans	521:553	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	3	2	from	findings	570:577	arg1	construction					586:597	the construction	582:597	the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	582:679	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	4	3	theme	tools	921:925	arg1	development					870:880	the development	866:880	the development of therapeutic vaccines and diagnostic tools	866:925	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	2	4	theme	system	375:380	arg1	reactivity					343:352	the reactivity	339:352	the reactivity of the glycosylation system	339:380	Reagent-controlled glycosylation methodologies, in which external nucleophiles are employed to modulate the reactivity of the glycosylation system, have become powerful means for the construction of 1,2-cis-glycosidic linkages.					
32141465	1	5	from	assembly	157:164	arg1	key					146:148	key	146:148	key	146:148	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	1	5	from	assembly	157:164	arg1	construction					99:110	The stereoselective construction	79:110	The stereoselective construction of 1,2-cis-glycosidic linkages	79:141	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	4	6	theme	structural	818:827	arg1	element					829:835	a structural element	816:835	a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools	816:925	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	1	7	from	key	146:148	arg1	assembly					157:164	the assembly	153:164	the assembly of biologically relevant glycans	153:197	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	4	8	theme	bacterium	768:776	arg1	wall					728:731	the cell wall	719:731	the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis	719:799	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	4	9	theme	Gram-positive	754:766	arg1	bacterium					768:776	the opportunistic Gram-positive bacterium	736:776	the opportunistic Gram-positive bacterium	736:776	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	4	9	theme	Gram-positive	754:766	arg1	faecalis					792:799	Enterococcus faecalis	779:799	Enterococcus faecalis	779:799	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	4	10	theme	vaccines	897:904	arg1	development					870:880	the development	866:880	the development of therapeutic vaccines and diagnostic tools	866:925	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	2	11	theme	external	292:299	arg1	nucleophiles					301:312	external nucleophiles	292:312	external nucleophiles	292:312	Reagent-controlled glycosylation methodologies, in which external nucleophiles are employed to modulate the reactivity of the glycosylation system, have become powerful means for the construction of 1,2-cis-glycosidic linkages.					
32141465	4	12	theme	therapeutic	885:895	arg1	vaccines					897:904	therapeutic vaccines	885:904	therapeutic vaccines	885:904	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	4	13	theme	diagnostic	910:919	arg1	tools					921:925	diagnostic tools	910:925	diagnostic tools	910:925	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	1	14	theme	relevant	182:189	arg1	glycans					191:197	biologically relevant glycans	169:197	biologically relevant glycans	169:197	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	3	15	theme	d-alanine	604:612	arg1	fragment					672:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	2	16	theme	powerful	395:402	arg1	means					404:408	powerful means	395:408	powerful means for the construction of 1,2-cis-glycosidic linkages	395:460	Reagent-controlled glycosylation methodologies, in which external nucleophiles are employed to modulate the reactivity of the glycosylation system, have become powerful means for the construction of 1,2-cis-glycosidic linkages.					
32141465	1	17	theme	stereoselective	83:97	arg1	key					146:148	key	146:148	key	146:148	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	1	17	theme	stereoselective	83:97	arg1	construction					99:110	The stereoselective construction	79:110	The stereoselective construction of 1,2-cis-glycosidic linkages	79:141	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	3	18	theme	glycerol	639:646	arg1	fragment					672:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	2	19	theme	linkages	453:460	arg1	construction					418:429	the construction	414:429	the construction of 1,2-cis-glycosidic linkages	414:460	Reagent-controlled glycosylation methodologies, in which external nucleophiles are employed to modulate the reactivity of the glycosylation system, have become powerful means for the construction of 1,2-cis-glycosidic linkages.					
32141465	0	20	theme	stereoselective	19:33	arg1	synthesis					35:43	stereoselective synthesis	19:43	stereoselective synthesis of teichoic acid α-(1,2)-glucans	19:76	Reagent controlled stereoselective synthesis of teichoic acid α-(1,2)-glucans.					
32141465	2	21	theme	1,2-cis-glycosidic	434:451	arg1	linkages					453:460	1,2-cis-glycosidic linkages	434:460	1,2-cis-glycosidic linkages	434:460	Reagent-controlled glycosylation methodologies, in which external nucleophiles are employed to modulate the reactivity of the glycosylation system, have become powerful means for the construction of 1,2-cis-glycosidic linkages.					
32141465	0	22	theme	teichoic	48:55	arg1	-glucans					69:76	teichoic acid α-(1,2)-glucans	48:76	teichoic acid α-(1,2)-glucans	48:76	Reagent controlled stereoselective synthesis of teichoic acid α-(1,2)-glucans.					
32141465	4	23	theme	opportunistic	740:752	arg1	bacterium					768:776	the opportunistic Gram-positive bacterium	736:776	the opportunistic Gram-positive bacterium	736:776	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	4	23	theme	opportunistic	740:752	arg1	faecalis					792:799	Enterococcus faecalis	779:799	Enterococcus faecalis	779:799	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	4	24	located	found	710:714	arg1	wall					728:731	the cell wall	719:731	the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis	719:799	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	4	24	located	found	710:714	arg2	molecule					694:701	This latter molecule	682:701	This latter molecule	682:701	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	2	25	theme	Reagent-controlled	235:252	arg1	methodologies					268:280	Reagent-controlled glycosylation methodologies	235:280	Reagent-controlled glycosylation methodologies	235:280	Reagent-controlled glycosylation methodologies, in which external nucleophiles are employed to modulate the reactivity of the glycosylation system, have become powerful means for the construction of 1,2-cis-glycosidic linkages.					
32141465	1	26	theme	glycans	191:197	arg1	assembly					157:164	the assembly	153:164	the assembly of biologically relevant glycans	153:197	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	3	27	theme	phosphate	648:656	arg1	fragment					672:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	3	28	theme	nucleophilic	486:497	arg1	additives					499:507	nucleophilic additives	486:507	nucleophilic additives	486:507	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	0	29	theme	α-	62:63	arg1	-glucans					69:76	teichoic acid α-(1,2)-glucans	48:76	teichoic acid α-(1,2)-glucans	48:76	Reagent controlled stereoselective synthesis of teichoic acid α-(1,2)-glucans.					
32141465	3	30	theme	kojibiose	614:622	arg1	fragment					672:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	2	31	theme	glycosylation	361:373	arg1	system					375:380	the glycosylation system	357:380	the glycosylation system	357:380	Reagent-controlled glycosylation methodologies, in which external nucleophiles are employed to modulate the reactivity of the glycosylation system, have become powerful means for the construction of 1,2-cis-glycosidic linkages.					
32141465	0	32	theme	acid	57:60	arg1	-glucans					69:76	teichoic acid α-(1,2)-glucans	48:76	teichoic acid α-(1,2)-glucans	48:76	Reagent controlled stereoselective synthesis of teichoic acid α-(1,2)-glucans.					
32141465	3	33	theme	functionalized	624:637	arg1	fragment					672:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	1	34	theme	1,2-cis-glycosidic	115:132	arg1	linkages					134:141	1,2-cis-glycosidic linkages	115:141	1,2-cis-glycosidic linkages	115:141	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	3	35	theme	acid	667:670	arg1	fragment					672:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	3	36	theme	fragment	672:679	arg1	construction					586:597	the construction	582:597	the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	582:679	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	1	37	theme	linkages	134:141	arg1	key					146:148	key	146:148	key	146:148	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	1	37	theme	linkages	134:141	arg1	construction					99:110	The stereoselective construction	79:110	The stereoselective construction of 1,2-cis-glycosidic linkages	79:141	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	1	38	theme	synthetic	214:222	arg1	challenge					224:232	a synthetic challenge	212:232	a synthetic challenge	212:232	The stereoselective construction of 1,2-cis-glycosidic linkages is key in the assembly of biologically relevant glycans, but remains a synthetic challenge.					
32141465	4	39	used	used	858:861	arg2	element					829:835	a structural element	816:835	a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools	816:925	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	0	40	theme	1,2	65:67	arg1	-glucans					69:76	teichoic acid α-(1,2)-glucans	48:76	teichoic acid α-(1,2)-glucans	48:76	Reagent controlled stereoselective synthesis of teichoic acid α-(1,2)-glucans.					
32141465	3	41	theme	teichoic	658:665	arg1	fragment					672:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment	602:679	Here we establish that nucleophilic additives can support the construction of α-1,2-glucans, and apply our findings in the construction of a d-alanine kojibiose functionalized glycerol phosphate teichoic acid fragment.					
32141465	4	42	theme	latter	687:692	arg1	molecule					694:701	This latter molecule	682:701	This latter molecule	682:701	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
32141465	2	43	theme	glycosylation	254:266	arg1	methodologies					268:280	Reagent-controlled glycosylation methodologies	235:280	Reagent-controlled glycosylation methodologies	235:280	Reagent-controlled glycosylation methodologies, in which external nucleophiles are employed to modulate the reactivity of the glycosylation system, have become powerful means for the construction of 1,2-cis-glycosidic linkages.					
32141465	4	44	theme	cell	723:726	arg1	wall					728:731	the cell wall	719:731	the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis	719:799	This latter molecule can be found in the cell wall of the opportunistic Gram-positive bacterium, Enterococcus faecalis and represents a structural element that can possibly be used in the development of therapeutic vaccines and diagnostic tools.					
34634304	0	0	theme	BPNT2	99:103	arg1	in vitro					105:112	lithium-inhibited phosphatase BPNT2 in vitro	69:112	lithium-inhibited phosphatase BPNT2 in vitro	69:112	Sulfation of glycosaminoglycans depends on the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro.					
34634304	6	1	theme	GAG	970:972	arg1	in vitro					984:991	GAG sulfation in vitro	970:991	GAG sulfation in vitro	970:991	Here, we used mouse embryonic fibroblasts (MEFs) to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro.					
34634304	3	2	theme	sulfation	559:567	arg1	abnormalities					521:533	skeletal abnormalities	512:533	skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development	512:666	KO of BPNT2 in mice leads to skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development.					
34634304	0	3	theme	phosphatase	87:97	arg1	in vitro					105:112	lithium-inhibited phosphatase BPNT2 in vitro	69:112	lithium-inhibited phosphatase BPNT2 in vitro	69:112	Sulfation of glycosaminoglycans depends on the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro.					
34634304	9	4	theme	GAG	1514:1516	arg1	sulfation					1518:1526	GAG sulfation	1514:1526	GAG sulfation	1514:1526	We further show that treatment of MEFs with lithium (a common psychotropic medication) inhibits GAG sulfation and that this effect depends on the presence of BPNT2.					
34634304	7	5	theme	catalytic-dead	1009:1022	arg1	D108A					1041:1045	D108A	1041:1045	D108A	1041:1045	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	7	5	theme	catalytic-dead	1009:1022	arg1	construct					1030:1038	a catalytic-dead Bpnt2 construct	1007:1038	a catalytic-dead Bpnt2 construct (D108A)	1007:1046	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	2	6	theme	3'-phosphoadenosine-5'-phosphate	398:429	arg1	breakdown					385:393	the breakdown	381:393	the breakdown of 3'-phosphoadenosine-5'-phosphate, a by-product of glycosaminoglycan (GAG) sulfation	381:480	BPNT2 catalyzes the breakdown of 3'-phosphoadenosine-5'-phosphate, a by-product of glycosaminoglycan (GAG) sulfation.					
34634304	3	7	from	KO	483:484	arg1	mice					498:501	mice	498:501	mice	498:501	KO of BPNT2 in mice leads to skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development.					
34634304	10	8	theme	new	1778:1780	arg1	insights					1782:1789	new insights	1778:1789	new insights into lithium pharmacology	1778:1815	Taken together, this work demonstrates that the catalytic activity of an enzyme potently inhibited by lithium can modulate GAG sulfation and therefore extracellular matrix composition, revealing new insights into lithium pharmacology.					
34634304	7	9	theme	Bpnt2	1024:1028	arg1	D108A					1041:1045	D108A	1041:1045	D108A	1041:1045	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	7	9	theme	Bpnt2	1024:1028	arg1	construct					1030:1038	a catalytic-dead Bpnt2 construct	1007:1038	a catalytic-dead Bpnt2 construct (D108A)	1007:1046	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	3	10	theme	proper	628:633	arg1	development					656:666	proper extracellular matrix development	628:666	proper extracellular matrix development	628:666	KO of BPNT2 in mice leads to skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development.					
34634304	9	11	theme	common	1473:1478	arg1	medication					1493:1502	a common psychotropic medication	1471:1502	a common psychotropic medication	1471:1502	We further show that treatment of MEFs with lithium (a common psychotropic medication) inhibits GAG sulfation and that this effect depends on the presence of BPNT2.					
34634304	9	11	theme	common	1473:1478	arg1	lithium					1462:1468	lithium	1462:1468	lithium (a common psychotropic medication)	1462:1503	We further show that treatment of MEFs with lithium (a common psychotropic medication) inhibits GAG sulfation and that this effect depends on the presence of BPNT2.					
34634304	0	12	theme	in vitro	105:112	arg1	activity					57:64	the catalytic activity	43:64	the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro	43:112	Sulfation of glycosaminoglycans depends on the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro.					
34634304	7	13	theme	Bpnt2-KO	1174:1181	arg1	MEFs					1183:1186	Bpnt2-KO MEFs	1174:1186	Bpnt2-KO MEFs	1174:1186	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	8	14	theme	MEF	1404:1406	arg1	cultures					1408:1415	MEF cultures	1404:1415	MEF cultures	1404:1415	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	8	15	theme	overall	1351:1357	arg1	sulfation					1363:1371	overall GAG sulfation	1351:1371	overall GAG sulfation	1351:1371	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	6	16	theme	BPNT2	948:952	arg1	activity					936:943	the catalytic activity	922:943	the catalytic activity of BPNT2	922:952	Here, we used mouse embryonic fibroblasts (MEFs) to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro.					
34634304	6	17	theme	embryonic	865:873	arg1	fibroblasts					875:885	mouse embryonic fibroblasts	859:885	mouse embryonic fibroblasts (MEFs)	859:892	Here, we used mouse embryonic fibroblasts (MEFs) to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro.					
34634304	6	17	theme	embryonic	865:873	arg1	MEFs					888:891	MEFs	888:891	MEFs	888:891	Here, we used mouse embryonic fibroblasts (MEFs) to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro.					
34634304	6	18	used	used	854:857	arg2	we					851:852	we	851:852	we	851:852	Here, we used mouse embryonic fibroblasts (MEFs) to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro.					
34634304	10	19	theme	GAG	1706:1708	arg1	sulfation					1710:1718	GAG sulfation	1706:1718	GAG sulfation	1706:1718	Taken together, this work demonstrates that the catalytic activity of an enzyme potently inhibited by lithium can modulate GAG sulfation and therefore extracellular matrix composition, revealing new insights into lithium pharmacology.					
34634304	9	20	theme	psychotropic	1480:1491	arg1	medication					1493:1502	a common psychotropic medication	1471:1502	a common psychotropic medication	1471:1502	We further show that treatment of MEFs with lithium (a common psychotropic medication) inhibits GAG sulfation and that this effect depends on the presence of BPNT2.					
34634304	9	20	theme	psychotropic	1480:1491	arg1	lithium					1462:1468	lithium	1462:1468	lithium (a common psychotropic medication)	1462:1503	We further show that treatment of MEFs with lithium (a common psychotropic medication) inhibits GAG sulfation and that this effect depends on the presence of BPNT2.					
34634304	7	21	theme	present	1163:1169	arg1	chondroitin-4-sulfate					1140:1160	decreased chondroitin-4-sulfate	1130:1160	decreased chondroitin-4-sulfate	1130:1160	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	6	22	theme	mouse	859:863	arg1	fibroblasts					875:885	mouse embryonic fibroblasts	859:885	mouse embryonic fibroblasts (MEFs)	859:892	Here, we used mouse embryonic fibroblasts (MEFs) to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro.					
34634304	6	22	theme	mouse	859:863	arg1	MEFs					888:891	MEFs	888:891	MEFs	888:891	Here, we used mouse embryonic fibroblasts (MEFs) to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro.					
34634304	10	23	theme	matrix	1748:1753	arg1	composition					1755:1765	extracellular matrix composition	1734:1765	extracellular matrix composition	1734:1765	Taken together, this work demonstrates that the catalytic activity of an enzyme potently inhibited by lithium can modulate GAG sulfation and therefore extracellular matrix composition, revealing new insights into lithium pharmacology.					
34634304	4	24	from	disorder	741:748	arg1	humans					753:758	humans	753:758	humans	753:758	Mutations in BPNT2 have also been found to underlie a chondrodysplastic disorder in humans.					
34634304	1	25	theme	family	183:188	arg1	a member					169:176	a member	169:176	a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis	169:362	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	9	26	theme	BPNT2	1576:1580	arg1	presence					1564:1571	the presence	1560:1571	the presence of BPNT2	1560:1580	We further show that treatment of MEFs with lithium (a common psychotropic medication) inhibits GAG sulfation and that this effect depends on the presence of BPNT2.					
34634304	7	27	from	present	1163:1169	arg1	MEFs					1183:1186	Bpnt2-KO MEFs	1174:1186	Bpnt2-KO MEFs	1174:1186	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	7	28	theme	decreased	1130:1138	arg1	chondroitin-4-sulfate					1140:1160	decreased chondroitin-4-sulfate	1130:1160	decreased chondroitin-4-sulfate	1130:1160	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	8	29	from	chondroitin-4-sulfation	1377:1399	arg1	cultures					1408:1415	MEF cultures	1404:1415	MEF cultures	1404:1415	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	3	30	theme	extracellular	635:647	arg1	development					656:666	proper extracellular matrix development	628:666	proper extracellular matrix development	628:666	KO of BPNT2 in mice leads to skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development.					
34634304	2	31	theme	glycosaminoglycan	448:464	arg1	sulfation					472:480	glycosaminoglycan (GAG) sulfation	448:480	glycosaminoglycan (GAG) sulfation	448:480	BPNT2 catalyzes the breakdown of 3'-phosphoadenosine-5'-phosphate, a by-product of glycosaminoglycan (GAG) sulfation.					
34634304	8	32	theme	adjacent	1242:1249	arg1	Bpnt2					1236:1240	Bpnt2	1236:1240	Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans,	1236:1325	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	1	33	theme	metal	319:323	arg1	binding					325:331	metal binding	319:331	metal binding to effect phosphate hydrolysis	319:362	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	3	34	theme	matrix	649:654	arg1	development					656:666	proper extracellular matrix development	628:666	proper extracellular matrix development	628:666	KO of BPNT2 in mice leads to skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development.					
34634304	1	35	theme	magnesium-dependent	193:211	arg1	phosphatases					232:243	magnesium-dependent, lithium-inhibited phosphatases	193:243	magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis	193:362	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	8	36	theme	catalytic	1258:1266	arg1	site					1268:1271	the catalytic site	1254:1271	the catalytic site	1254:1271	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	8	37	from	defects	1340:1346	arg1	chondroitin-4-sulfation					1377:1399	chondroitin-4-sulfation	1377:1399	chondroitin-4-sulfation	1377:1399	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	8	37	from	defects	1340:1346	arg1	sulfation					1363:1371	overall GAG sulfation	1351:1371	overall GAG sulfation	1351:1371	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	1	38	theme	Golgi-resident	115:128	arg1	nucleotidase					143:154	Golgi-resident bisphosphate nucleotidase 2	115:156	Golgi-resident bisphosphate nucleotidase 2 (BPNT2)	115:164	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	1	38	theme	Golgi-resident	115:128	arg1	BPNT2					159:163	BPNT2	159:163	BPNT2	159:163	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	3	39	theme	skeletal	512:519	arg1	abnormalities					521:533	skeletal abnormalities	512:533	skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development	512:666	KO of BPNT2 in mice leads to skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development.					
34634304	0	40	theme	glycosaminoglycans	13:30	arg1	Sulfation					0:8	Sulfation	0:8	Sulfation of glycosaminoglycans	0:30	Sulfation of glycosaminoglycans depends on the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro.					
34634304	6	41	theme	catalytic	926:934	arg1	activity					936:943	the catalytic activity	922:943	the catalytic activity of BPNT2	922:952	Here, we used mouse embryonic fibroblasts (MEFs) to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro.					
34634304	1	42	theme	bisphosphate	130:141	arg1	nucleotidase					143:154	Golgi-resident bisphosphate nucleotidase 2	115:156	Golgi-resident bisphosphate nucleotidase 2 (BPNT2)	115:164	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	1	42	theme	bisphosphate	130:141	arg1	BPNT2					159:163	BPNT2	159:163	BPNT2	159:163	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	1	43	theme	effect	336:341	arg1	hydrolysis					353:362	effect phosphate hydrolysis	336:362	effect phosphate hydrolysis	336:362	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	7	44	theme	sulfated	1105:1112	arg1	GAGs					1114:1117	intracellular or secreted sulfated GAGs	1079:1117	GAGs	1114:1117	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	7	44	theme	sulfated	1105:1112	arg1	chondroitin-4-sulfate					1140:1160	decreased chondroitin-4-sulfate	1130:1160	decreased chondroitin-4-sulfate	1130:1160	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	1	45	theme	phosphatases	232:243	arg1	family					183:188	a family	181:188	a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis	181:362	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	3	46	theme	BPNT2	489:493	arg1	KO					483:484	KO	483:484	KO of BPNT2 in mice	483:501	KO of BPNT2 in mice leads to skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development.					
34634304	2	47	theme	sulfation	472:480	arg1	3'-phosphoadenosine-5'-phosphate					398:429	3'-phosphoadenosine-5'-phosphate	398:429	3'-phosphoadenosine-5'-phosphate	398:429	BPNT2 catalyzes the breakdown of 3'-phosphoadenosine-5'-phosphate, a by-product of glycosaminoglycan (GAG) sulfation.					
34634304	2	47	theme	sulfation	472:480	arg1	by-product					434:443	a by-product	432:443	a by-product of glycosaminoglycan (GAG) sulfation	432:480	BPNT2 catalyzes the breakdown of 3'-phosphoadenosine-5'-phosphate, a by-product of glycosaminoglycan (GAG) sulfation.					
34634304	1	48	theme	phosphate	343:351	arg1	hydrolysis					353:362	effect phosphate hydrolysis	336:362	effect phosphate hydrolysis	336:362	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	4	49	from	Mutations	669:677	arg1	BPNT2					682:686	BPNT2	682:686	BPNT2	682:686	Mutations in BPNT2 have also been found to underlie a chondrodysplastic disorder in humans.					
34634304	1	50	dep	magnesium-dependent	193:211	arg1	lithium-inhibited					214:230	lithium-inhibited	214:230	lithium-inhibited	214:230	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	8	51	from	chondrodysplasia	1299:1314	arg1	humans					1319:1324	humans	1319:1324	humans	1319:1324	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	7	52	from	impairments	1064:1074	arg1	GAGs					1114:1117	intracellular or secreted sulfated GAGs	1079:1117	GAGs	1114:1117	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	7	52	from	impairments	1064:1074	arg1	chondroitin-4-sulfate					1140:1160	decreased chondroitin-4-sulfate	1130:1160	decreased chondroitin-4-sulfate	1130:1160	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	3	53	theme	impaired	546:553	arg1	sulfation					559:567	impaired GAG sulfation	546:567	impaired GAG sulfation	546:567	KO of BPNT2 in mice leads to skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development.					
34634304	0	54	theme	catalytic	47:55	arg1	activity					57:64	the catalytic activity	43:64	the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro	43:112	Sulfation of glycosaminoglycans depends on the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro.					
34634304	7	55	from	MEFs	1183:1186	arg1	present					1163:1169	present	1163:1169	present	1163:1169	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	7	56	theme	secreted	1096:1103	arg1	GAGs					1114:1117	intracellular or secreted sulfated GAGs	1079:1117	GAGs	1114:1117	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	7	56	theme	secreted	1096:1103	arg1	chondroitin-4-sulfate					1140:1160	decreased chondroitin-4-sulfate	1130:1160	decreased chondroitin-4-sulfate	1130:1160	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	3	57	theme	GAG	555:557	arg1	sulfation					559:567	impaired GAG sulfation	546:567	impaired GAG sulfation	546:567	KO of BPNT2 in mice leads to skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development.					
34634304	7	58	attach	present	1163:1169	arg1	MEFs					1183:1186	Bpnt2-KO MEFs	1174:1186	Bpnt2-KO MEFs	1174:1186	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	7	58	attach	present	1163:1169	arg2	chondroitin-4-sulfate					1140:1160	decreased chondroitin-4-sulfate	1130:1160	decreased chondroitin-4-sulfate	1130:1160	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	8	59	from	sulfation	1363:1371	arg1	cultures					1408:1415	MEF cultures	1404:1415	MEF cultures	1404:1415	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	5	60	theme	precise	765:771	arg1	mechanism					773:781	The precise mechanism	761:781	The precise mechanism by which the loss of BPNT2 impairs sulfation	761:826	The precise mechanism by which the loss of BPNT2 impairs sulfation remains unclear.					
34634304	8	61	theme	missense	1214:1221	arg1	mutations					1223:1231	missense mutations	1214:1231	missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans,	1214:1325	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	9	62	with	treatment	1439:1447	arg1	medication					1493:1502	a common psychotropic medication	1471:1502	a common psychotropic medication	1471:1502	We further show that treatment of MEFs with lithium (a common psychotropic medication) inhibits GAG sulfation and that this effect depends on the presence of BPNT2.					
34634304	9	62	with	treatment	1439:1447	arg1	lithium					1462:1468	lithium	1462:1468	lithium (a common psychotropic medication)	1462:1503	We further show that treatment of MEFs with lithium (a common psychotropic medication) inhibits GAG sulfation and that this effect depends on the presence of BPNT2.					
34634304	8	63	theme	GAG	1359:1361	arg1	sulfation					1363:1371	overall GAG sulfation	1351:1371	overall GAG sulfation	1351:1371	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	7	64	theme	intracellular	1079:1091	arg1	GAGs					1114:1117	intracellular or secreted sulfated GAGs	1079:1117	GAGs	1114:1117	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	7	64	theme	intracellular	1079:1091	arg1	chondroitin-4-sulfate					1140:1160	decreased chondroitin-4-sulfate	1130:1160	decreased chondroitin-4-sulfate	1130:1160	We show that a catalytic-dead Bpnt2 construct (D108A) does not rescue impairments in intracellular or secreted sulfated GAGs, including decreased chondroitin-4-sulfate, present in Bpnt2-KO MEFs.					
34634304	8	65	from	mutations	1223:1231	arg1	Bpnt2					1236:1240	Bpnt2	1236:1240	Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans,	1236:1325	We also demonstrate that missense mutations in Bpnt2 adjacent to the catalytic site, which are known to cause chondrodysplasia in humans, recapitulate defects in overall GAG sulfation and chondroitin-4-sulfation in MEF cultures.					
34634304	5	66	theme	BPNT2	804:808	arg1	loss					796:799	the loss	792:799	the loss of BPNT2	792:808	The precise mechanism by which the loss of BPNT2 impairs sulfation remains unclear.					
34634304	9	67	theme	MEFs	1452:1455	arg1	treatment					1439:1447	treatment	1439:1447	treatment of MEFs with lithium (a common psychotropic medication)	1439:1503	We further show that treatment of MEFs with lithium (a common psychotropic medication) inhibits GAG sulfation and that this effect depends on the presence of BPNT2.					
34634304	0	68	theme	lithium-inhibited	69:85	arg1	in vitro					105:112	lithium-inhibited phosphatase BPNT2 in vitro	69:112	lithium-inhibited phosphatase BPNT2 in vitro	69:112	Sulfation of glycosaminoglycans depends on the catalytic activity of lithium-inhibited phosphatase BPNT2 in vitro.					
34634304	10	69	theme	enzyme	1656:1661	arg1	activity					1641:1648	the catalytic activity	1627:1648	the catalytic activity of an enzyme potently inhibited by lithium	1627:1691	Taken together, this work demonstrates that the catalytic activity of an enzyme potently inhibited by lithium can modulate GAG sulfation and therefore extracellular matrix composition, revealing new insights into lithium pharmacology.					
34634304	10	70	theme	extracellular	1734:1746	arg1	composition					1755:1765	extracellular matrix composition	1734:1765	extracellular matrix composition	1734:1765	Taken together, this work demonstrates that the catalytic activity of an enzyme potently inhibited by lithium can modulate GAG sulfation and therefore extracellular matrix composition, revealing new insights into lithium pharmacology.					
34634304	10	71	theme	lithium	1796:1802	arg1	pharmacology					1804:1815	lithium pharmacology	1796:1815	lithium pharmacology	1796:1815	Taken together, this work demonstrates that the catalytic activity of an enzyme potently inhibited by lithium can modulate GAG sulfation and therefore extracellular matrix composition, revealing new insights into lithium pharmacology.					
34634304	4	72	theme	chondrodysplastic	723:739	arg1	disorder					741:748	a chondrodysplastic disorder	721:748	a chondrodysplastic disorder in humans	721:758	Mutations in BPNT2 have also been found to underlie a chondrodysplastic disorder in humans.					
34634304	1	73	theme	three-dimensional	258:274	arg1	motif					287:291	a three-dimensional structural motif	256:291	a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis	256:362	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
34634304	6	74	theme	sulfation	974:982	arg1	in vitro					984:991	GAG sulfation in vitro	970:991	GAG sulfation in vitro	970:991	Here, we used mouse embryonic fibroblasts (MEFs) to test the hypothesis that the catalytic activity of BPNT2 is required for GAG sulfation in vitro.					
34634304	10	75	theme	catalytic	1631:1639	arg1	activity					1641:1648	the catalytic activity	1627:1648	the catalytic activity of an enzyme potently inhibited by lithium	1627:1691	Taken together, this work demonstrates that the catalytic activity of an enzyme potently inhibited by lithium can modulate GAG sulfation and therefore extracellular matrix composition, revealing new insights into lithium pharmacology.					
34634304	3	76	theme	chondroitin-4-sulfation	581:603	arg1	sulfation					559:567	impaired GAG sulfation	546:567	impaired GAG sulfation	546:567	KO of BPNT2 in mice leads to skeletal abnormalities because of impaired GAG sulfation, especially chondroitin-4-sulfation, which is critical for proper extracellular matrix development.					
34634304	1	77	theme	structural	276:285	arg1	motif					287:291	a three-dimensional structural motif	256:291	a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis	256:362	Golgi-resident bisphosphate nucleotidase 2 (BPNT2) is a member of a family of magnesium-dependent, lithium-inhibited phosphatases that share a three-dimensional structural motif that directly coordinates metal binding to effect phosphate hydrolysis.					
33103248	4	0	theme	acidic	570:575	arg1	conditions					577:586	acidic conditions	570:586	acidic conditions	570:586	The enzyme catalyzed the hydrolysis of different xylans under acidic conditions and was stable in the pH range 2.6-4.5.					
33103248	9	1	theme	xylooligosaccharides	1687:1706	arg1	synthesis					1665:1673	the synthesis	1661:1673	the synthesis of valuable xylooligosaccharides	1661:1706	The acidophilic properties and high transglycosylation activity make ScXynA a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides.					
33103248	6	2	theme	efficient	1027:1035	arg1	production					1037:1046	the efficient production	1023:1046	the efficient production of o-nitrophenyl xylooligosaccharides	1023:1084	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	7	3	theme	ScXynA	1224:1229	arg1	structure					1231:1239	The modeled ScXynA structure	1212:1239	The modeled ScXynA structure	1212:1239	The modeled ScXynA structure showed a favorable position for ligand entry and o-nitrophenyl group accommodation in the relatively open -3 subsite, while the cleavage site was covered with an extended loop.					
33103248	9	4	theme	acidophilic	1518:1528	arg1	properties					1530:1539	The acidophilic properties	1514:1539	The acidophilic properties	1514:1539	The acidophilic properties and high transglycosylation activity make ScXynA a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides.					
33103248	1	5	theme	xylans	185:190	arg1	conversion					171:180	the conversion	167:180	the conversion of xylans in plant biomass	167:207	Endo-β-xylanases are hemicellulases involved in the conversion of xylans in plant biomass.					
33103248	0	6	theme	xylooligosaccharides	97:116	arg1	synthesis					70:78	the synthesis	66:78	the synthesis of o-nitrophenyl xylooligosaccharides	66:116	A novel acid-tolerant β-xylanase from Scytalidium candidum 3C for the synthesis of o-nitrophenyl xylooligosaccharides.					
33103248	6	7	dep	donor	987:991	arg1	both					977:980	both	977:980	both	977:980	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	5	8	theme	ScXynA	654:659	arg1	parameters					640:649	The kinetic parameters	628:649	The kinetic parameters of ScXynA	628:659	The kinetic parameters of ScXynA were determined in hydrolysis reactions with p-nitrophenyl-β-d-cellobioside (pNP-β-Cel) and p-nitrophenyl-β-d-xylobioside (pNP-β-Xyl2 ), and kcat /Km was found to be 0.43 ± 0.02 (s·mM)-1 and 57 ± 3 (s·mM)-1 , respectively.					
33103248	7	9	theme	group	1304:1308	arg1	accommodation					1310:1322	o-nitrophenyl group accommodation	1290:1322	o-nitrophenyl group accommodation	1290:1322	The modeled ScXynA structure showed a favorable position for ligand entry and o-nitrophenyl group accommodation in the relatively open -3 subsite, while the cleavage site was covered with an extended loop.					
33103248	9	10	theme	various	1612:1618	arg1	synthesis					1665:1673	the synthesis	1661:1673	the synthesis of valuable xylooligosaccharides	1661:1706	The acidophilic properties and high transglycosylation activity make ScXynA a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides.					
33103248	9	10	theme	various	1612:1618	arg1	applications					1637:1648	various biotechnological applications	1612:1648	various biotechnological applications	1612:1648	The acidophilic properties and high transglycosylation activity make ScXynA a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides.					
33103248	0	11	theme	o-nitrophenyl	83:95	arg1	xylooligosaccharides					97:116	o-nitrophenyl xylooligosaccharides	83:116	o-nitrophenyl xylooligosaccharides	83:116	A novel acid-tolerant β-xylanase from Scytalidium candidum 3C for the synthesis of o-nitrophenyl xylooligosaccharides.					
33103248	0	12	from	3C	59:60	arg1	β-xylanase					22:31	A novel acid-tolerant β-xylanase	0:31	A novel acid-tolerant β-xylanase from Scytalidium candidum 3C for the synthesis of o-nitrophenyl xylooligosaccharides.	0:117	A novel acid-tolerant β-xylanase from Scytalidium candidum 3C for the synthesis of o-nitrophenyl xylooligosaccharides.					
33103248	6	13	theme	major	1183:1187	arg1	yield					1204:1208	18.5% yield	1198:1208	18.5% yield	1198:1208	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	6	13	theme	major	1183:1187	arg1	product					1189:1195	the major product	1179:1195	the major product (18.5% yield)	1179:1209	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	6	14	theme	polymerization	1104:1117	arg1	degree					1094:1099	a degree	1092:1099	a degree of polymerization of 3-10	1092:1125	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	6	14	theme	polymerization	1104:1117	arg1	oNP-β-Xyl4					1163:1172	oNP-β-Xyl4	1163:1172	oNP-β-Xyl4	1163:1172	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	6	14	theme	polymerization	1104:1117	arg1	o-nitrophenyl-β-d-xylotetraose					1131:1160	o-nitrophenyl-β-d-xylotetraose	1131:1160	o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 )	1131:1174	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	3	15	theme	38 ± 5 kDa	484:493	arg1	weight					474:479	a molecular weight	462:479	a molecular weight of 38 ± 5 kDa per subunit	462:505	ScXynA was identified as a glycoside hydrolase family 10 (GH10) dimeric protein, with a molecular weight of 38 ± 5 kDa per subunit.					
33103248	1	16	from	conversion	171:180	arg1	biomass					201:207	plant biomass	195:207	plant biomass	195:207	Endo-β-xylanases are hemicellulases involved in the conversion of xylans in plant biomass.					
33103248	2	17	theme	transglycosylation	276:293	arg1	abilities					295:303	high transglycosylation abilities	271:303	high transglycosylation abilities	271:303	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	7	18	theme	open	1342:1345	arg1	subsite					1350:1356	the relatively open -3 subsite	1327:1356	the relatively open -3 subsite	1327:1356	The modeled ScXynA structure showed a favorable position for ligand entry and o-nitrophenyl group accommodation in the relatively open -3 subsite, while the cleavage site was covered with an extended loop.					
33103248	3	19	theme	dimeric	440:446	arg1	protein					448:454	a glycoside hydrolase family 10 (GH10) dimeric protein	401:454	a glycoside hydrolase family 10 (GH10) dimeric protein	401:454	ScXynA was identified as a glycoside hydrolase family 10 (GH10) dimeric protein, with a molecular weight of 38 ± 5 kDa per subunit.					
33103248	3	19	theme	dimeric	440:446	arg1	ScXynA					376:381	ScXynA	376:381	ScXynA	376:381	ScXynA was identified as a glycoside hydrolase family 10 (GH10) dimeric protein, with a molecular weight of 38 ± 5 kDa per subunit.					
33103248	5	20	theme	kcat	802:805	arg1	/Km					807:809	kcat /Km	802:809	kcat /Km	802:809	The kinetic parameters of ScXynA were determined in hydrolysis reactions with p-nitrophenyl-β-d-cellobioside (pNP-β-Cel) and p-nitrophenyl-β-d-xylobioside (pNP-β-Xyl2 ), and kcat /Km was found to be 0.43 ± 0.02 (s·mM)-1 and 57 ± 3 (s·mM)-1 , respectively.					
33103248	2	21	theme	high	271:274	arg1	abilities					295:303	high transglycosylation abilities	271:303	high transglycosylation abilities	271:303	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	1	22	theme	plant	195:199	arg1	biomass					201:207	plant biomass	195:207	plant biomass	195:207	Endo-β-xylanases are hemicellulases involved in the conversion of xylans in plant biomass.					
33103248	0	23	theme	novel	2:6	arg1	β-xylanase					22:31	A novel acid-tolerant β-xylanase	0:31	A novel acid-tolerant β-xylanase from Scytalidium candidum 3C for the synthesis of o-nitrophenyl xylooligosaccharides.	0:117	A novel acid-tolerant β-xylanase from Scytalidium candidum 3C for the synthesis of o-nitrophenyl xylooligosaccharides.					
33103248	9	24	theme	suitable	1592:1599	arg1	choice					1601:1606	a suitable choice	1590:1606	a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides	1590:1706	The acidophilic properties and high transglycosylation activity make ScXynA a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides.					
33103248	6	25	theme	o-nitrophenyl	1051:1063	arg1	xylooligosaccharides					1065:1084	o-nitrophenyl xylooligosaccharides	1051:1084	o-nitrophenyl xylooligosaccharides	1051:1084	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	3	26	theme	glycoside	403:411	arg1	family					423:428	glycoside hydrolase family 10	403:431	glycoside hydrolase family 10 (GH10)	403:438	ScXynA was identified as a glycoside hydrolase family 10 (GH10) dimeric protein, with a molecular weight of 38 ± 5 kDa per subunit.					
33103248	3	26	theme	glycoside	403:411	arg1	GH10					434:437	GH10	434:437	GH10	434:437	ScXynA was identified as a glycoside hydrolase family 10 (GH10) dimeric protein, with a molecular weight of 38 ± 5 kDa per subunit.					
33103248	4	27	theme	xylans	557:562	arg1	hydrolysis					533:542	the hydrolysis	529:542	the hydrolysis of different xylans	529:562	The enzyme catalyzed the hydrolysis of different xylans under acidic conditions and was stable in the pH range 2.6-4.5.					
33103248	5	28	theme	kinetic	632:638	arg1	parameters					640:649	The kinetic parameters	628:649	The kinetic parameters of ScXynA	628:659	The kinetic parameters of ScXynA were determined in hydrolysis reactions with p-nitrophenyl-β-d-cellobioside (pNP-β-Cel) and p-nitrophenyl-β-d-xylobioside (pNP-β-Xyl2 ), and kcat /Km was found to be 0.43 ± 0.02 (s·mM)-1 and 57 ± 3 (s·mM)-1 , respectively.					
33103248	2	29	dep	filamentous	332:342	arg1	fungus					344:349	fungus	344:349	fungus	344:349	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	8	30	theme	structural	1424:1433	arg1	features					1435:1442	These structural features	1418:1442	These structural features	1418:1442	These structural features provide favorable conditions for transglycosylation with oNP-β-Xyl2 .					
33103248	4	31	theme	different	547:555	arg1	xylans					557:562	different xylans	547:562	different xylans	547:562	The enzyme catalyzed the hydrolysis of different xylans under acidic conditions and was stable in the pH range 2.6-4.5.					
33103248	6	32	theme	xylooligosaccharides	1065:1084	arg1	production					1037:1046	the efficient production	1023:1046	the efficient production of o-nitrophenyl xylooligosaccharides	1023:1084	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	7	33	theme	o-nitrophenyl	1290:1302	arg1	accommodation					1310:1322	o-nitrophenyl group accommodation	1290:1322	o-nitrophenyl group accommodation	1290:1322	The modeled ScXynA structure showed a favorable position for ligand entry and o-nitrophenyl group accommodation in the relatively open -3 subsite, while the cleavage site was covered with an extended loop.					
33103248	0	34	theme	acid-tolerant	8:20	arg1	β-xylanase					22:31	A novel acid-tolerant β-xylanase	0:31	A novel acid-tolerant β-xylanase from Scytalidium candidum 3C for the synthesis of o-nitrophenyl xylooligosaccharides.	0:117	A novel acid-tolerant β-xylanase from Scytalidium candidum 3C for the synthesis of o-nitrophenyl xylooligosaccharides.					
33103248	2	35	with	β-xylanase	246:255	arg1	abilities					295:303	high transglycosylation abilities	271:303	high transglycosylation abilities	271:303	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	7	36	theme	extended	1403:1410	arg1	loop					1412:1415	an extended loop	1400:1415	an extended loop	1400:1415	The modeled ScXynA structure showed a favorable position for ligand entry and o-nitrophenyl group accommodation in the relatively open -3 subsite, while the cleavage site was covered with an extended loop.					
33103248	9	37	theme	transglycosylation	1550:1567	arg1	activity					1569:1576	high transglycosylation activity	1545:1576	high transglycosylation activity	1545:1576	The acidophilic properties and high transglycosylation activity make ScXynA a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides.					
33103248	3	38	theme	molecular	464:472	arg1	weight					474:479	a molecular weight	462:479	a molecular weight of 38 ± 5 kDa per subunit	462:505	ScXynA was identified as a glycoside hydrolase family 10 (GH10) dimeric protein, with a molecular weight of 38 ± 5 kDa per subunit.					
33103248	0	39	theme	Scytalidium	38:48	arg1	3C					59:60	Scytalidium candidum 3C	38:60	Scytalidium candidum 3C	38:60	A novel acid-tolerant β-xylanase from Scytalidium candidum 3C for the synthesis of o-nitrophenyl xylooligosaccharides.					
33103248	2	40	attach	isolated	314:321	arg2	ScXynA					258:263	ScXynA	258:263	ScXynA	258:263	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	2	40	attach	isolated	314:321	arg2	β-xylanase					246:255	a novel acidophilic β-xylanase	226:255	a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C	226:373	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	2	40	attach	isolated	314:321	arg1	3C					372:373	the filamentous fungus Scytalidium candidum 3C	328:373	the filamentous fungus Scytalidium candidum 3C	328:373	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	6	41	theme	3-10	1122:1125	arg1	polymerization					1104:1117	polymerization	1104:1117	polymerization of 3-10	1104:1125	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	7	42	theme	cleavage	1369:1376	arg1	site					1378:1381	the cleavage site	1365:1381	the cleavage site	1365:1381	The modeled ScXynA structure showed a favorable position for ligand entry and o-nitrophenyl group accommodation in the relatively open -3 subsite, while the cleavage site was covered with an extended loop.					
33103248	9	43	theme	high	1545:1548	arg1	activity					1569:1576	high transglycosylation activity	1545:1576	high transglycosylation activity	1545:1576	The acidophilic properties and high transglycosylation activity make ScXynA a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides.					
33103248	8	44	theme	favorable	1452:1460	arg1	conditions					1462:1471	favorable conditions	1452:1471	favorable conditions for transglycosylation with oNP-β-Xyl2	1452:1510	These structural features provide favorable conditions for transglycosylation with oNP-β-Xyl2 .					
33103248	2	45	theme	acidophilic	234:244	arg1	ScXynA					258:263	ScXynA	258:263	ScXynA	258:263	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	2	45	theme	acidophilic	234:244	arg1	β-xylanase					246:255	a novel acidophilic β-xylanase	226:255	a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C	226:373	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	3	46	theme	hydrolase	413:421	arg1	family					423:428	glycoside hydrolase family 10	403:431	glycoside hydrolase family 10 (GH10)	403:438	ScXynA was identified as a glycoside hydrolase family 10 (GH10) dimeric protein, with a molecular weight of 38 ± 5 kDa per subunit.					
33103248	3	46	theme	hydrolase	413:421	arg1	GH10					434:437	GH10	434:437	GH10	434:437	ScXynA was identified as a glycoside hydrolase family 10 (GH10) dimeric protein, with a molecular weight of 38 ± 5 kDa per subunit.					
33103248	4	47	from	stable	596:601	arg1	range					613:617	the pH range 2.6-4.5	606:625	the pH range 2.6-4.5	606:625	The enzyme catalyzed the hydrolysis of different xylans under acidic conditions and was stable in the pH range 2.6-4.5.					
33103248	2	48	theme	novel	228:232	arg1	ScXynA					258:263	ScXynA	258:263	ScXynA	258:263	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	2	48	theme	novel	228:232	arg1	β-xylanase					246:255	a novel acidophilic β-xylanase	226:255	a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C	226:373	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	2	49	theme	candidum	363:370	arg1	3C					372:373	the filamentous fungus Scytalidium candidum 3C	328:373	the filamentous fungus Scytalidium candidum 3C	328:373	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	0	50	theme	candidum	50:57	arg1	3C					59:60	Scytalidium candidum 3C	38:60	Scytalidium candidum 3C	38:60	A novel acid-tolerant β-xylanase from Scytalidium candidum 3C for the synthesis of o-nitrophenyl xylooligosaccharides.					
33103248	6	51	theme	transglycosylation	908:925	arg1	o-nitrophenyl-β-d-xylobioside					927:955	the transglycosylation o-nitrophenyl-β-d-xylobioside	904:955	the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 )	904:969	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	6	51	theme	transglycosylation	908:925	arg1	oNP-β-Xyl2					958:967	oNP-β-Xyl2	958:967	oNP-β-Xyl2	958:967	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	2	52	theme	Scytalidium	351:361	arg1	3C					372:373	the filamentous fungus Scytalidium candidum 3C	328:373	the filamentous fungus Scytalidium candidum 3C	328:373	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	9	53	theme	valuable	1678:1685	arg1	xylooligosaccharides					1687:1706	valuable xylooligosaccharides	1678:1706	valuable xylooligosaccharides	1678:1706	The acidophilic properties and high transglycosylation activity make ScXynA a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides.					
33103248	5	54	theme	hydrolysis	680:689	arg1	reactions					691:699	hydrolysis reactions	680:699	hydrolysis reactions with p-nitrophenyl-β-d-cellobioside (pNP-β-Cel) and p-nitrophenyl-β-d-xylobioside (pNP-β-Xyl2 )	680:795	The kinetic parameters of ScXynA were determined in hydrolysis reactions with p-nitrophenyl-β-d-cellobioside (pNP-β-Cel) and p-nitrophenyl-β-d-xylobioside (pNP-β-Xyl2 ), and kcat /Km was found to be 0.43 ± 0.02 (s·mM)-1 and 57 ± 3 (s·mM)-1 , respectively.					
33103248	7	55	theme	favorable	1250:1258	arg1	position					1260:1267	a favorable position	1248:1267	a favorable position for ligand entry and o-nitrophenyl group accommodation	1248:1322	The modeled ScXynA structure showed a favorable position for ligand entry and o-nitrophenyl group accommodation in the relatively open -3 subsite, while the cleavage site was covered with an extended loop.					
33103248	3	56	with	protein	448:454	arg1	weight					474:479	a molecular weight	462:479	a molecular weight of 38 ± 5 kDa per subunit	462:505	ScXynA was identified as a glycoside hydrolase family 10 (GH10) dimeric protein, with a molecular weight of 38 ± 5 kDa per subunit.					
33103248	6	57	theme	%	1202:1202	arg1	yield					1204:1208	18.5% yield	1198:1208	18.5% yield	1198:1208	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	6	57	theme	%	1202:1202	arg1	product					1189:1195	the major product	1179:1195	the major product (18.5% yield)	1179:1209	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	2	58	theme	filamentous	332:342	arg1	3C					372:373	the filamentous fungus Scytalidium candidum 3C	328:373	the filamentous fungus Scytalidium candidum 3C	328:373	Here, we report a novel acidophilic β-xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C.					
33103248	4	59	from	range	613:617	arg1	stable					596:601	stable	596:601	stable	596:601	The enzyme catalyzed the hydrolysis of different xylans under acidic conditions and was stable in the pH range 2.6-4.5.					
33103248	4	59	from	range	613:617	arg1	enzyme					512:517	The enzyme	508:517	The enzyme	508:517	The enzyme catalyzed the hydrolysis of different xylans under acidic conditions and was stable in the pH range 2.6-4.5.					
33103248	6	60	theme	18.5	1198:1201	arg1	%					1202:1202	%	1202:1202	%	1202:1202	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	5	61	with	reactions	691:699	arg1	p-nitrophenyl-β-d-xylobioside					753:781	p-nitrophenyl-β-d-xylobioside	753:781	p-nitrophenyl-β-d-xylobioside (pNP-β-Xyl2 )	753:795	The kinetic parameters of ScXynA were determined in hydrolysis reactions with p-nitrophenyl-β-d-cellobioside (pNP-β-Cel) and p-nitrophenyl-β-d-xylobioside (pNP-β-Xyl2 ), and kcat /Km was found to be 0.43 ± 0.02 (s·mM)-1 and 57 ± 3 (s·mM)-1 , respectively.					
33103248	5	61	with	reactions	691:699	arg1	p-nitrophenyl-β-d-cellobioside					706:735	p-nitrophenyl-β-d-cellobioside	706:735	p-nitrophenyl-β-d-cellobioside (pNP-β-Cel)	706:747	The kinetic parameters of ScXynA were determined in hydrolysis reactions with p-nitrophenyl-β-d-cellobioside (pNP-β-Cel) and p-nitrophenyl-β-d-xylobioside (pNP-β-Xyl2 ), and kcat /Km was found to be 0.43 ± 0.02 (s·mM)-1 and 57 ± 3 (s·mM)-1 , respectively.					
33103248	5	61	with	reactions	691:699	arg1	pNP-β-Xyl2					784:793	pNP-β-Xyl2	784:793	pNP-β-Xyl2	784:793	The kinetic parameters of ScXynA were determined in hydrolysis reactions with p-nitrophenyl-β-d-cellobioside (pNP-β-Cel) and p-nitrophenyl-β-d-xylobioside (pNP-β-Xyl2 ), and kcat /Km was found to be 0.43 ± 0.02 (s·mM)-1 and 57 ± 3 (s·mM)-1 , respectively.					
33103248	5	61	with	reactions	691:699	arg1	pNP-β-Cel					738:746	pNP-β-Cel	738:746	pNP-β-Cel	738:746	The kinetic parameters of ScXynA were determined in hydrolysis reactions with p-nitrophenyl-β-d-cellobioside (pNP-β-Cel) and p-nitrophenyl-β-d-xylobioside (pNP-β-Xyl2 ), and kcat /Km was found to be 0.43 ± 0.02 (s·mM)-1 and 57 ± 3 (s·mM)-1 , respectively.					
33103248	9	62	theme	biotechnological	1620:1635	arg1	synthesis					1665:1673	the synthesis	1661:1673	the synthesis of valuable xylooligosaccharides	1661:1706	The acidophilic properties and high transglycosylation activity make ScXynA a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides.					
33103248	9	62	theme	biotechnological	1620:1635	arg1	applications					1637:1648	various biotechnological applications	1612:1648	various biotechnological applications	1612:1648	The acidophilic properties and high transglycosylation activity make ScXynA a suitable choice for various biotechnological applications, including the synthesis of valuable xylooligosaccharides.					
33103248	7	63	theme	modeled	1216:1222	arg1	structure					1231:1239	The modeled ScXynA structure	1212:1239	The modeled ScXynA structure	1212:1239	The modeled ScXynA structure showed a favorable position for ligand entry and o-nitrophenyl group accommodation in the relatively open -3 subsite, while the cleavage site was covered with an extended loop.					
33103248	7	64	theme	ligand	1273:1278	arg1	entry					1280:1284	ligand entry	1273:1284	ligand entry	1273:1284	The modeled ScXynA structure showed a favorable position for ligand entry and o-nitrophenyl group accommodation in the relatively open -3 subsite, while the cleavage site was covered with an extended loop.					
33103248	8	65	with	transglycosylation	1477:1494	arg1	oNP-β-Xyl2					1501:1510	oNP-β-Xyl2	1501:1510	oNP-β-Xyl2	1501:1510	These structural features provide favorable conditions for transglycosylation with oNP-β-Xyl2 .					
33103248	6	66	theme	o-nitrophenyl-β-d-xylobioside	927:955	arg1	catalysis					891:899	the catalysis	887:899	the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 )	887:969	In the catalysis of the transglycosylation o-nitrophenyl-β-d-xylobioside (oNP-β-Xyl2 ) acted both as a donor and an acceptor, resulting in the efficient production of o-nitrophenyl xylooligosaccharides, with a degree of polymerization of 3-10 and o-nitrophenyl-β-d-xylotetraose (oNP-β-Xyl4 ) as the major product (18.5% yield).					
33103248	4	67	theme	pH	610:611	arg1	range					613:617	the pH range 2.6-4.5	606:625	the pH range 2.6-4.5	606:625	The enzyme catalyzed the hydrolysis of different xylans under acidic conditions and was stable in the pH range 2.6-4.5.					
33111073	2	0	theme	self-renewable	348:361	arg1	array					339:343	the cell-based glycan array	317:343	the cell-based glycan array	317:343	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	2	0	theme	self-renewable	348:361	arg1	resource					363:370	a self-renewable resource	346:370	a self-renewable resource for the display of the human glycome in the natural context	346:430	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	4	1	dep	use	642:644	arg1	the					638:640	the	638:640	the	638:640	For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).					
33111073	2	2	theme	features	289:296	arg1	gain					267:270	gain	267:270	gain	267:270	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	2	2	theme	features	289:296	arg1	loss					259:262	loss	259:262	loss	259:262	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	3	3	theme	biological	541:550	arg1	functions					552:560	biological functions	541:560	biological functions	541:560	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	2	4	theme	glycosylation	275:287	arg1	features					289:296	glycosylation features	275:296	glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context	275:430	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	3	5	theme	molecular	514:522	arg1	interactions					524:535	molecular interactions	514:535	molecular interactions	514:535	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	1	6	theme	tremendous	161:170	arg1	diversity					183:191	its tremendous structural diversity	157:191	its tremendous structural diversity	157:191	Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity.					
33111073	3	7	theme	interactions	524:535	arg1	dissection					500:509	dissection	500:509	dissection of molecular interactions and biological functions of glycans using a wide range of biological assays	500:611	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	1	8	theme	structural	172:181	arg1	diversity					183:191	its tremendous structural diversity	157:191	its tremendous structural diversity	157:191	Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity.					
33111073	0	9	theme	Glycan	11:16	arg1	Guide					37:41	Cell-Based Glycan Arrays-A Practical Guide	0:41	Cell-Based Glycan Arrays-A Practical Guide	0:41	Cell-Based Glycan Arrays-A Practical Guide to Dissect the Human Glycome.					
33111073	3	10	theme	glycans	565:571	arg1	dissection					500:509	dissection	500:509	dissection of molecular interactions and biological functions of glycans using a wide range of biological assays	500:611	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	0	11	theme	Cell-Based	0:9	arg1	Guide					37:41	Cell-Based Glycan Arrays-A Practical Guide	0:41	Cell-Based Glycan Arrays-A Practical Guide	0:41	Cell-Based Glycan Arrays-A Practical Guide to Dissect the Human Glycome.					
33111073	1	12	theme	biological	87:96	arg1	functions					98:106	the biological functions	83:106	the biological functions of the human glycome	83:127	Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity.					
33111073	0	13	theme	Practical	27:35	arg1	Guide					37:41	Cell-Based Glycan Arrays-A Practical Guide	0:41	Cell-Based Glycan Arrays-A Practical Guide	0:41	Cell-Based Glycan Arrays-A Practical Guide to Dissect the Human Glycome.					
33111073	2	14	with	libraries	219:227	arg1	gain					267:270	gain	267:270	gain	267:270	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	2	14	with	libraries	219:227	arg1	loss					259:262	loss	259:262	loss	259:262	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	3	15	theme	functions	552:560	arg1	dissection					500:509	dissection	500:509	dissection of molecular interactions and biological functions of glycans using a wide range of biological assays	500:611	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	2	16	theme	natural	416:422	arg1	context					424:430	the natural context	412:430	the natural context	412:430	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	4	17	dep	refer	685:689	arg1	Narimatsu					695:703	Narimatsu	695:703	Narimatsu	695:703	For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).					
33111073	4	18	from	details	627:633	arg1	execution					650:658	execution	650:658	execution	650:658	For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).					
33111073	4	18	from	details	627:633	arg1	use					642:644	use	642:644	use	642:644	For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).					
33111073	4	19	theme	complete	618:625	arg1	details					627:633	complete details	618:633	complete details on the use and execution of this protocol	618:675	For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).					
33111073	3	20	theme	array	490:494	arg1	use					461:463	the use	457:463	the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays	457:611	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	3	21	theme	glycan	483:488	arg1	array					490:494	the cell-based glycan array	468:494	the cell-based glycan array	468:494	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	2	22	theme	HEK293	241:246	arg1	cells					248:252	isogenic HEK293 cells	232:252	isogenic HEK293 cells	232:252	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	2	23	theme	glycome	401:407	arg1	display					380:386	the display	376:386	the display of the human glycome in the natural context	376:430	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	0	24	theme	Arrays-A	18:25	arg1	Guide					37:41	Cell-Based Glycan Arrays-A Practical Guide	0:41	Cell-Based Glycan Arrays-A Practical Guide	0:41	Cell-Based Glycan Arrays-A Practical Guide to Dissect the Human Glycome.					
33111073	2	25	theme	isogenic	232:239	arg1	cells					248:252	isogenic HEK293 cells	232:252	isogenic HEK293 cells	232:252	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	2	26	from	display	380:386	arg1	context					424:430	the natural context	412:430	the natural context	412:430	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	2	27	theme	human	395:399	arg1	glycome					401:407	the human glycome	391:407	the human glycome	391:407	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	3	28	theme	cell-based	472:481	arg1	array					490:494	the cell-based glycan array	468:494	the cell-based glycan array	468:494	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	2	29	theme	stable	212:217	arg1	libraries					219:227	stable libraries	212:227	stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context	212:430	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	3	30	theme	wide	581:584	arg1	range					586:590	a wide range	579:590	a wide range of biological assays	579:611	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	1	31	theme	human	115:119	arg1	glycome					121:127	the human glycome	111:127	the human glycome	111:127	Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity.					
33111073	3	32	theme	assays	606:611	arg1	range					586:590	a wide range	579:590	a wide range of biological assays	579:611	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	1	33	theme	glycome	121:127	arg1	functions					98:106	the biological functions	83:106	the biological functions of the human glycome	83:127	Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity.					
33111073	2	34	theme	cell-based	321:330	arg1	array					339:343	the cell-based glycan array	317:343	the cell-based glycan array	317:343	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	2	34	theme	cell-based	321:330	arg1	resource					363:370	a self-renewable resource	346:370	a self-renewable resource for the display of the human glycome in the natural context	346:430	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	2	35	theme	glycan	332:337	arg1	array					339:343	the cell-based glycan array	317:343	the cell-based glycan array	317:343	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	2	35	theme	glycan	332:337	arg1	resource					363:370	a self-renewable resource	346:370	a self-renewable resource for the display of the human glycome in the natural context	346:430	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
33111073	3	36	theme	biological	595:604	arg1	assays					606:611	biological assays	595:611	biological assays	595:611	This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays.					
33111073	4	37	theme	protocol	668:675	arg1	execution					650:658	execution	650:658	execution	650:658	For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).					
33111073	4	37	theme	protocol	668:675	arg1	use					642:644	use	642:644	use	642:644	For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).					
33111073	0	38	theme	Human	58:62	arg1	Glycome					64:70	the Human Glycome	54:70	the Human Glycome	54:70	Cell-Based Glycan Arrays-A Practical Guide to Dissect the Human Glycome.					
33111073	2	39	theme	cells	248:252	arg1	libraries					219:227	stable libraries	212:227	stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context	212:430	We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context.					
32438810	0	0	theme	Overall	71:77	arg1	Whey					86:89	the Overall Bovine Whey N-Linked Glycoprofile	67:111	the Overall Bovine Whey N-Linked Glycoprofile	67:111	In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile.					
32438810	7	1	theme	protein	1193:1199	arg1	changes					1215:1221	dynamic protein concentration changes	1185:1221	dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time	1185:1266	Application of the whey glycoprofile analysis to colostrum samples revealed dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time.					
32438810	1	2	theme	whey	150:153	arg1	result					171:176	the combined result	158:176	the combined result of individual protein glycoprofiles	158:212	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	1	2	theme	whey	150:153	arg1	glycoprofile					127:138	glycoprofile	127:138	glycoprofile	127:138	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	6	3	theme	Signature	963:971	arg1	glycans					973:979	Signature glycans	963:979	Signature glycans	963:979	Signature glycans can be used to recognize individual proteins in the overall whey glycoprofile as well as for protein concentration estimations.					
32438810	7	4	theme	concentration	1201:1213	arg1	changes					1215:1221	dynamic protein concentration changes	1185:1221	dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time	1185:1266	Application of the whey glycoprofile analysis to colostrum samples revealed dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time.					
32438810	5	5	with	glycoprotein	852:863	arg1	degree					923:928	a high degree	916:928	a high degree of fucosylation and sialylation	916:960	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	5	6	theme	whey	847:850	arg1	glycoprotein					852:863	the sole whey glycoprotein	838:863	the sole whey glycoprotein carrying tri- and tetra-antennary structures	838:908	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	5	6	theme	whey	847:850	arg1	GlyCAM-1					826:833	GlyCAM-1	826:833	GlyCAM-1	826:833	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	6	7	theme	concentration	1082:1094	arg1	estimations					1096:1106	protein concentration estimations	1074:1106	protein concentration estimations	1074:1106	Signature glycans can be used to recognize individual proteins in the overall whey glycoprofile as well as for protein concentration estimations.					
32438810	4	8	with	glycans	778:784	arg1	motifs					818:823	Gal-β(1,4)-GlcNAc (LacNAc) motifs	791:823	Gal-β(1,4)-GlcNAc (LacNAc) motifs	791:823	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	4	9	theme	fucosylated	753:763	arg1	glycans					778:784	fucosylated di-antennary glycans	753:784	fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs	753:823	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	2	10	theme	glycan	279:284	arg1	structures					286:295	the glycan structures	275:295	the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	275:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	3	11	from	present	510:516	arg1	glycoprofile					538:549	glycoprofile	538:549	glycoprofile	538:549	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	0	12	theme	Bovine	79:84	arg1	Whey					86:89	the Overall Bovine Whey N-Linked Glycoprofile	67:111	the Overall Bovine Whey N-Linked Glycoprofile	67:111	In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile.					
32438810	3	13	theme	present	510:516	arg1	N-glycans					500:508	N-glycans	500:508	N-glycans present in the overall whey glycoprofile	500:549	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	5	14	theme	tetra-antennary	883:897	arg1	structures					899:908	tri- and tetra-antennary structures	874:908	tri- and tetra-antennary structures	874:908	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	0	15	theme	Glycoprofile	100:111	arg1	Whey					86:89	the Overall Bovine Whey N-Linked Glycoprofile	67:111	the Overall Bovine Whey N-Linked Glycoprofile	67:111	In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile.					
32438810	5	16	theme	sialylation	950:960	arg1	degree					923:928	a high degree	916:928	a high degree of fucosylation and sialylation	916:960	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	7	17	theme	glycoprofile	1133:1144	arg1	analysis					1146:1153	the whey glycoprofile analysis	1124:1153	the whey glycoprofile analysis	1124:1153	Application of the whey glycoprofile analysis to colostrum samples revealed dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time.					
32438810	3	18	dep	proteins	576:583	arg1	proteins					576:583	three proteins	570:583	three proteins: lactoferrin, IgG, and GlyCAM-1	570:615	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	3	18	dep	proteins	576:583	arg1	IgG					599:601	IgG	599:601	IgG	599:601	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	3	18	dep	proteins	576:583	arg1	GlyCAM-1					608:615	GlyCAM-1	608:615	GlyCAM-1	608:615	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	3	18	dep	proteins	576:583	arg1	lactoferrin					586:596	lactoferrin	586:596	lactoferrin	586:596	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	6	19	theme	individual	1006:1015	arg1	proteins					1017:1024	individual proteins	1006:1024	individual proteins	1006:1024	Signature glycans can be used to recognize individual proteins in the overall whey glycoprofile as well as for protein concentration estimations.					
32438810	0	20	theme	N-Linked	91:98	arg1	Whey					86:89	the Overall Bovine Whey N-Linked Glycoprofile	67:111	the Overall Bovine Whey N-Linked Glycoprofile	67:111	In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile.					
32438810	4	21	gly	fucosylated	753:763	arg1	glycans					778:784	fucosylated di-antennary glycans	753:784	fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs	753:823	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	5	22	theme	fucosylation	933:944	arg1	degree					923:928	a high degree	916:928	a high degree of fucosylation and sialylation	916:960	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	1	23	theme	combined	162:169	arg1	result					171:176	the combined result	158:176	the combined result of individual protein glycoprofiles	158:212	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	1	23	theme	combined	162:169	arg1	glycoprofile					127:138	glycoprofile	127:138	glycoprofile	127:138	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	0	24	theme	Depth	3:7	arg1	Analysis					9:16	Depth Analysis	3:16	Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile	3:111	In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile.					
32438810	2	25	dep	molecule	449:456	arg1	PP3					471:473	PP3	471:473	PP3	471:473	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	25	dep	molecule	449:456	arg1	GlyCAM-1					461:468	GlyCAM-1	461:468	GlyCAM-1	461:468	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	26	theme	whey	306:309	arg1	immunoglobulin-G					379:394	immunoglobulin-G	379:394	immunoglobulin-G (IgG)	379:400	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	26	theme	whey	306:309	arg1	lactoferrin					334:344	lactoferrin	334:344	lactoferrin	334:344	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	26	theme	whey	306:309	arg1	molecule					449:456	glycosylation-dependent cellular adhesion molecule 1	407:458	glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	407:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	26	theme	whey	306:309	arg1	lactoperoxidase					347:361	lactoperoxidase	347:361	lactoperoxidase	347:361	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	26	theme	whey	306:309	arg1	glycoproteins					311:323	known whey glycoproteins	300:323	known whey glycoproteins	300:323	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	26	theme	whey	306:309	arg1	α-lactalbumin					364:376	α-lactalbumin	364:376	α-lactalbumin	364:376	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	7	27	theme	analysis	1146:1153	arg1	Application					1109:1119	Application	1109:1119	Application of the whey glycoprofile analysis to colostrum samples	1109:1174	Application of the whey glycoprofile analysis to colostrum samples revealed dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time.					
32438810	2	28	theme	adhesion	440:447	arg1	glycoproteins					311:323	known whey glycoproteins	300:323	known whey glycoproteins	300:323	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	28	theme	adhesion	440:447	arg1	molecule					449:456	glycosylation-dependent cellular adhesion molecule 1	407:458	glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	407:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	4	29	theme	LacNAc	810:815	arg1	motifs					818:823	Gal-β(1,4)-GlcNAc (LacNAc) motifs	791:823	Gal-β(1,4)-GlcNAc (LacNAc) motifs	791:823	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	2	30	theme	known	300:304	arg1	immunoglobulin-G					379:394	immunoglobulin-G	379:394	immunoglobulin-G (IgG)	379:400	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	30	theme	known	300:304	arg1	lactoferrin					334:344	lactoferrin	334:344	lactoferrin	334:344	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	30	theme	known	300:304	arg1	molecule					449:456	glycosylation-dependent cellular adhesion molecule 1	407:458	glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	407:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	30	theme	known	300:304	arg1	lactoperoxidase					347:361	lactoperoxidase	347:361	lactoperoxidase	347:361	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	30	theme	known	300:304	arg1	glycoproteins					311:323	known whey glycoproteins	300:323	known whey glycoproteins	300:323	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	30	theme	known	300:304	arg1	α-lactalbumin					364:376	α-lactalbumin	364:376	α-lactalbumin	364:376	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	3	31	attach	present	510:516	arg1	glycoprofile					538:549	glycoprofile	538:549	glycoprofile	538:549	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	3	31	attach	present	510:516	arg2	N-glycans					500:508	N-glycans	500:508	N-glycans present in the overall whey glycoprofile	500:549	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	2	32	theme	cellular	431:438	arg1	glycoproteins					311:323	known whey glycoproteins	300:323	known whey glycoproteins	300:323	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	32	theme	cellular	431:438	arg1	molecule					449:456	glycosylation-dependent cellular adhesion molecule 1	407:458	glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	407:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	1	33	theme	individual	181:190	arg1	glycoprofiles					200:212	individual protein glycoprofiles	181:212	individual protein glycoprofiles	181:212	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	2	34	theme	glycosylation-dependent	407:429	arg1	glycoproteins					311:323	known whey glycoproteins	300:323	known whey glycoproteins	300:323	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	34	theme	glycosylation-dependent	407:429	arg1	molecule					449:456	glycosylation-dependent cellular adhesion molecule 1	407:458	glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	407:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	4	35	theme	oligomannose-type	708:724	arg1	glycans					726:732	oligomannose-type glycans	708:732	oligomannose-type glycans	708:732	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	1	36	theme	protein	192:198	arg1	glycoprofiles					200:212	individual protein glycoprofiles	181:212	individual protein glycoprofiles	181:212	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	0	37	theme	Contribution	25:36	arg1	Analysis					9:16	Depth Analysis	3:16	Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile	3:111	In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile.					
32438810	5	38	theme	high	918:921	arg1	degree					923:928	a high degree	916:928	a high degree of fucosylation and sialylation	916:960	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	7	39	theme	colostrum	1158:1166	arg1	samples					1168:1174	colostrum samples	1158:1174	colostrum samples	1158:1174	Application of the whey glycoprofile analysis to colostrum samples revealed dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time.					
32438810	1	40	theme	glycoprofiles	200:212	arg1	result					171:176	the combined result	158:176	the combined result of individual protein glycoprofiles	158:212	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	1	40	theme	glycoprofiles	200:212	arg1	glycoprofile					127:138	glycoprofile	127:138	glycoprofile	127:138	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	4	41	theme	Gal-β	791:795	arg1	motifs					818:823	Gal-β(1,4)-GlcNAc (LacNAc) motifs	791:823	Gal-β(1,4)-GlcNAc (LacNAc) motifs	791:823	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	0	42	theme	Specific	41:48	arg1	Glycoproteins					50:62	Specific Glycoproteins	41:62	Specific Glycoproteins	41:62	In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile.					
32438810	4	43	contain	carries	745:751	arg1	IgG					741:743	IgG	741:743	IgG	741:743	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	4	43	contain	carries	745:751	arg2	glycans					778:784	fucosylated di-antennary glycans	753:784	fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs	753:823	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	1	44	link	N-linked	118:125	arg1	result					171:176	the combined result	158:176	the combined result of individual protein glycoprofiles	158:212	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	1	44	link	N-linked	118:125	arg1	glycoprofile					127:138	glycoprofile	127:138	glycoprofile	127:138	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	3	45	from	glycoprofile	538:549	arg1	present					510:516	present	510:516	present	510:516	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	5	46	gly	glycoprotein	852:863	arg1	glycoprotein					852:863	the sole whey glycoprotein	838:863	the sole whey glycoprotein carrying tri- and tetra-antennary structures	838:908	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	5	46	gly	glycoprotein	852:863	arg1	GlyCAM-1					826:833	GlyCAM-1	826:833	GlyCAM-1	826:833	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	4	47	theme	main	669:672	arg1	proteins					674:681	these main proteins	663:681	these main proteins	663:681	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	4	48	theme	-GlcNAc	801:807	arg1	motifs					818:823	Gal-β(1,4)-GlcNAc (LacNAc) motifs	791:823	Gal-β(1,4)-GlcNAc (LacNAc) motifs	791:823	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	4	49	theme	1,4	797:799	arg1	motifs					818:823	Gal-β(1,4)-GlcNAc (LacNAc) motifs	791:823	Gal-β(1,4)-GlcNAc (LacNAc) motifs	791:823	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	5	50	theme	tri-	874:877	arg1	structures					899:908	tri- and tetra-antennary structures	874:908	tri- and tetra-antennary structures	874:908	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	7	51	theme	whey	1128:1131	arg1	analysis					1146:1153	the whey glycoprofile analysis	1124:1153	the whey glycoprofile analysis	1124:1153	Application of the whey glycoprofile analysis to colostrum samples revealed dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time.					
32438810	2	52	gly	glycoproteins	311:323	arg1	immunoglobulin-G					379:394	immunoglobulin-G	379:394	immunoglobulin-G (IgG)	379:400	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	52	gly	glycoproteins	311:323	arg1	lactoferrin					334:344	lactoferrin	334:344	lactoferrin	334:344	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	52	gly	glycoproteins	311:323	arg1	molecule					449:456	glycosylation-dependent cellular adhesion molecule 1	407:458	glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	407:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	52	gly	glycoproteins	311:323	arg1	lactoperoxidase					347:361	lactoperoxidase	347:361	lactoperoxidase	347:361	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	52	gly	glycoproteins	311:323	arg1	glycoproteins					311:323	known whey glycoproteins	300:323	known whey glycoproteins	300:323	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	52	gly	glycoproteins	311:323	arg1	α-lactalbumin					364:376	α-lactalbumin	364:376	α-lactalbumin	364:376	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	4	53	theme	di-antennary	765:776	arg1	glycans					778:784	fucosylated di-antennary glycans	753:784	fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs	753:823	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	2	54	theme	structural	249:258	arg1	information					260:270	in-depth structural information	240:270	in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	240:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	4	55	theme	specific	632:639	arg1	glycans					651:657	specific signature glycans	632:657	specific signature glycans for these main proteins	632:681	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	6	56	theme	protein	1074:1080	arg1	estimations					1096:1106	protein concentration estimations	1074:1106	protein concentration estimations	1074:1106	Signature glycans can be used to recognize individual proteins in the overall whey glycoprofile as well as for protein concentration estimations.					
32438810	2	57	from	information	260:270	arg1	structures					286:295	the glycan structures	275:295	the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	275:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	5	58	contain	carrying	865:872	arg2	structures					899:908	tri- and tetra-antennary structures	874:908	tri- and tetra-antennary structures	874:908	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	5	58	contain	carrying	865:872	arg1	glycoprotein					852:863	the sole whey glycoprotein	838:863	the sole whey glycoprotein carrying tri- and tetra-antennary structures	838:908	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	5	58	contain	carrying	865:872	arg1	GlyCAM-1					826:833	GlyCAM-1	826:833	GlyCAM-1	826:833	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	2	59	theme	in-depth	240:247	arg1	information					260:270	in-depth structural information	240:270	in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	240:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	2	60	theme	glycoproteins	311:323	arg1	structures					286:295	the glycan structures	275:295	the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3)	275:474	In this work, we provide in-depth structural information on the glycan structures of known whey glycoproteins, namely, lactoferrin, lactoperoxidase, α-lactalbumin, immunoglobulin-G (IgG), and glycosylation-dependent cellular adhesion molecule 1 (GlyCAM-1, PP3).					
32438810	5	61	theme	sole	842:845	arg1	glycoprotein					852:863	the sole whey glycoprotein	838:863	the sole whey glycoprotein carrying tri- and tetra-antennary structures	838:908	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	5	61	theme	sole	842:845	arg1	GlyCAM-1					826:833	GlyCAM-1	826:833	GlyCAM-1	826:833	GlyCAM-1 is the sole whey glycoprotein carrying tri- and tetra-antennary structures, with a high degree of fucosylation and sialylation.					
32438810	4	62	theme	signature	641:649	arg1	glycans					651:657	specific signature glycans	632:657	specific signature glycans for these main proteins	632:681	We identified specific signature glycans for these main proteins; lactoferrin contributes oligomannose-type glycans, while IgG carries fucosylated di-antennary glycans with Gal-β(1,4)-GlcNAc (LacNAc) motifs.					
32438810	0	63	theme	Glycoproteins	50:62	arg1	Contribution					25:36	the Contribution	21:36	the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile	21:111	In Depth Analysis of the Contribution of Specific Glycoproteins to the Overall Bovine Whey N-Linked Glycoprofile.					
32438810	3	64	theme	N-glycans	500:508	arg1	majority					481:488	The majority	477:488	The majority (∼95%) of N-glycans present in the overall whey glycoprofile	477:549	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	3	64	theme	N-glycans	500:508	arg1	%					494:494	∼95%	491:494	∼95%	491:494	The majority (∼95%) of N-glycans present in the overall whey glycoprofile were attributed to three proteins: lactoferrin, IgG, and GlyCAM-1.					
32438810	6	65	used	used	988:991	arg2	glycans					973:979	Signature glycans	963:979	Signature glycans	963:979	Signature glycans can be used to recognize individual proteins in the overall whey glycoprofile as well as for protein concentration estimations.					
32438810	1	66	theme	bovine	143:148	arg1	whey					150:153	bovine whey	143:153	bovine whey	143:153	The N-linked glycoprofile of bovine whey is the combined result of individual protein glycoprofiles.					
32438810	7	67	theme	dynamic	1185:1191	arg1	changes					1215:1221	dynamic protein concentration changes	1185:1221	dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time	1185:1266	Application of the whey glycoprofile analysis to colostrum samples revealed dynamic protein concentration changes for IgG, lactoferrin, and GlyCAM-1 over time.					
34672576	3	0	theme	-glycosidic	436:446	arg1	bonds					448:452	-glycosidic bonds	436:452	-glycosidic bonds	436:452	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	2	1	theme	disconnection	385:397	arg1	approach					399:406	a [3 + 2] disconnection approach	375:406	approach	399:406	The branched pentaglucolipid skeleton was achieved using a [3 + 2] disconnection approach.					
34672576	1	2	dep	-3-hydroxydecanoic	261:278	arg1	R					259:259	R	259:259	R	259:259	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	5	3	theme	functions	1062:1070	arg1	understanding					1028:1040	the understanding	1024:1040	the understanding of their biological functions in sponges	1024:1081	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	5	3	theme	functions	1062:1070	arg1	foundation					955:964	the foundation	951:964	the foundation for the total synthesis of sponge-derived agminosides	951:1018	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	1	4	theme	chimeric	152:159	arg1	glycolipid					161:170	a chimeric glycolipid	150:170	a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids	150:315	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	5	5	from	foundation	955:964	arg1	sponges					1075:1081	sponges	1075:1081	sponges	1075:1081	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	0	6	theme	Agminoside	101:110	arg1	E					112:112	Sponge-Derived Agminoside E	86:112	Sponge-Derived Agminoside E	86:112	Total Synthesis of a Chimeric Glycolipid Bearing the Partially Acetylated Backbone of Sponge-Derived Agminoside E.					
34672576	4	7	theme	continuous-flow	735:749	arg1	hydrogenolysis					764:777	continuous-flow microfluidic hydrogenolysis	735:777	continuous-flow microfluidic hydrogenolysis	735:777	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	0	8	theme	Sponge-Derived	86:99	arg1	E					112:112	Sponge-Derived Agminoside E	86:112	Sponge-Derived Agminoside E	86:112	Total Synthesis of a Chimeric Glycolipid Bearing the Partially Acetylated Backbone of Sponge-Derived Agminoside E.					
34672576	4	9	theme	2-azidomethyl	694:706	arg1	group					716:720	a (2-azidomethyl)benzoyl group	691:720	a (2-azidomethyl)benzoyl group	691:720	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	2	10	theme	branched	322:329	arg1	skeleton					347:354	The branched pentaglucolipid skeleton	318:354	The branched pentaglucolipid skeleton	318:354	The branched pentaglucolipid skeleton was achieved using a [3 + 2] disconnection approach.					
34672576	5	11	from	sponges	1075:1081	arg1	understanding					1028:1040	the understanding	1024:1040	the understanding of their biological functions in sponges	1024:1081	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	5	11	from	sponges	1075:1081	arg1	foundation					955:964	the foundation	951:964	the foundation for the total synthesis of sponge-derived agminosides	951:1018	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	4	12	theme	group	716:720	arg1	reduction					678:686	Staudinger reduction	667:686	Staudinger reduction of a (2-azidomethyl)benzoyl group	667:720	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	4	12	theme	group	716:720	arg1	pentaacetylation					649:664	Late-stage pentaacetylation	638:664	Late-stage pentaacetylation	638:664	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	1	13	theme	glycolipid	161:170	arg1	synthesis					137:145	the total synthesis	127:145	the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids	127:315	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	0	14	theme	E	112:112	arg1	Backbone					74:81	the Partially Acetylated Backbone	49:81	the Partially Acetylated Backbone of Sponge-Derived Agminoside E	49:112	Total Synthesis of a Chimeric Glycolipid Bearing the Partially Acetylated Backbone of Sponge-Derived Agminoside E.					
34672576	4	15	theme	glycolipid	840:849	arg1	synthesis					799:807	the total synthesis	789:807	the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E	789:933	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	4	16	theme	microfluidic	751:762	arg1	hydrogenolysis					764:777	continuous-flow microfluidic hydrogenolysis	735:777	continuous-flow microfluidic hydrogenolysis	735:777	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	5	17	link	sponge-derived	993:1006	arg1	agminosides					1008:1018	sponge-derived agminosides	993:1018	sponge-derived agminosides	993:1018	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	4	18	theme	partial	858:864	arg1	acetylation					866:876	acetylation	866:876	acetylation	866:876	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	5	19	theme	sponge-derived	993:1006	arg1	agminosides					1008:1018	sponge-derived agminosides	993:1018	sponge-derived agminosides	993:1018	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	3	20	theme	OTf	503:505	arg1	combination					481:491	a combination	479:491	a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors	479:635	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	0	21	theme	Total	0:4	arg1	Synthesis					6:14	Total Synthesis	0:14	Total Synthesis of a Chimeric Glycolipid	0:39	Total Synthesis of a Chimeric Glycolipid Bearing the Partially Acetylated Backbone of Sponge-Derived Agminoside E.					
34672576	1	22	theme	-3-hydroxydecanoic	261:278	arg1	chain					285:289	the (R)-3-hydroxydecanoic acid chain	254:289	the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids	254:315	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	3	23	dep	β-	413:414	arg1	bonds					448:452	-glycosidic bonds	436:452	-glycosidic bonds	436:452	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	1	24	link	sponge-derived	222:235	arg1	E					248:248	sponge-derived agminoside E	222:248	sponge-derived agminoside E	222:248	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	4	25	theme	benzoyl	708:714	arg1	group					716:720	a (2-azidomethyl)benzoyl group	691:720	a (2-azidomethyl)benzoyl group	691:720	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	5	26	from	understanding	1028:1040	arg1	sponges					1075:1081	sponges	1075:1081	sponges	1075:1081	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	3	27	gly	glycosylations	546:559	arg1	N-phenyltrifluoroacetimidate					575:602	N-phenyltrifluoroacetimidate	575:602	N-phenyltrifluoroacetimidate	575:602	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	3	27	gly	glycosylations	546:559	arg1	thiotolyl					564:572	thiotolyl	564:572	thiotolyl	564:572	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	3	27	gly	glycosylations	546:559	arg1	donors					630:635	trichloroacetimidate donors	609:635	trichloroacetimidate donors	609:635	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	1	28	theme	acid	280:283	arg1	chain					285:289	the (R)-3-hydroxydecanoic acid chain	254:289	the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids	254:315	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	4	29	theme	agminoside	922:931	arg1	E					933:933	agminoside E	922:933	agminoside E	922:933	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	3	30	theme	NIS/Yb	496:501	arg1	OTf					503:505	NIS/Yb(OTf)3-	496:508	NIS/Yb(OTf)3-	496:508	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	1	31	theme	acetylated	199:208	arg1	backbone					210:217	the partially acetylated backbone	185:217	the partially acetylated backbone of sponge-derived agminoside E	185:248	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	0	32	theme	Glycolipid	30:39	arg1	Synthesis					6:14	Total Synthesis	0:14	Total Synthesis of a Chimeric Glycolipid	0:39	Total Synthesis of a Chimeric Glycolipid Bearing the Partially Acetylated Backbone of Sponge-Derived Agminoside E.					
34672576	3	33	theme	trichloroacetimidate	609:628	arg1	donors					630:635	trichloroacetimidate donors	609:635	trichloroacetimidate donors	609:635	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	3	34	theme	TMSOTf-mediated	514:528	arg1	glycosylations					546:559	TMSOTf-mediated stereoselective glycosylations	514:559	TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors	514:635	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	4	35	theme	Late-stage	638:647	arg1	reduction					678:686	Staudinger reduction	667:686	Staudinger reduction of a (2-azidomethyl)benzoyl group	667:720	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	4	35	theme	Late-stage	638:647	arg1	pentaacetylation					649:664	Late-stage pentaacetylation	638:664	Late-stage pentaacetylation	638:664	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	0	36	theme	Chimeric	21:28	arg1	Glycolipid					30:39	a Chimeric Glycolipid	19:39	a Chimeric Glycolipid	19:39	Total Synthesis of a Chimeric Glycolipid Bearing the Partially Acetylated Backbone of Sponge-Derived Agminoside E.					
34672576	1	37	theme	bacterial	294:302	arg1	rhamnolipids					304:315	bacterial rhamnolipids	294:315	bacterial rhamnolipids	294:315	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	3	38	theme	N-phenyltrifluoroacetimidate	575:602	arg1	OTf					503:505	NIS/Yb(OTf)3-	496:508	NIS/Yb(OTf)3-	496:508	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	3	38	theme	N-phenyltrifluoroacetimidate	575:602	arg1	glycosylations					546:559	TMSOTf-mediated stereoselective glycosylations	514:559	TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors	514:635	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	2	39	dep	approach	399:406	arg1	2					382:382	2	382:382	2	382:382	The branched pentaglucolipid skeleton was achieved using a [3 + 2] disconnection approach.					
34672576	1	40	theme	rhamnolipids	304:315	arg1	chain					285:289	the (R)-3-hydroxydecanoic acid chain	254:289	the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids	254:315	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	1	40	theme	rhamnolipids	304:315	arg1	backbone					210:217	the partially acetylated backbone	185:217	the partially acetylated backbone of sponge-derived agminoside E	185:248	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	4	41	theme	simplified	829:838	arg1	glycolipid					840:849	the structurally simplified glycolipid	812:849	the structurally simplified glycolipid	812:849	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	4	42	from	pattern	878:884	arg1	part					900:903	the glycan part	889:903	the glycan part	889:903	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	4	43	theme	glycan	893:898	arg1	part					900:903	the glycan part	889:903	the glycan part	889:903	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	5	44	theme	agminosides	1008:1018	arg1	synthesis					980:988	the total synthesis	970:988	the total synthesis of sponge-derived agminosides	970:1018	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	5	45	theme	biological	1051:1060	arg1	functions					1062:1070	their biological functions	1045:1070	their biological functions in sponges	1045:1081	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	3	46	theme	thiotolyl	564:572	arg1	OTf					503:505	NIS/Yb(OTf)3-	496:508	NIS/Yb(OTf)3-	496:508	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	3	46	theme	thiotolyl	564:572	arg1	glycosylations					546:559	TMSOTf-mediated stereoselective glycosylations	514:559	TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors	514:635	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	3	47	theme	stereoselective	530:544	arg1	glycosylations					546:559	TMSOTf-mediated stereoselective glycosylations	514:559	TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors	514:635	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	2	48	theme	pentaglucolipid	331:345	arg1	skeleton					347:354	The branched pentaglucolipid skeleton	318:354	The branched pentaglucolipid skeleton	318:354	The branched pentaglucolipid skeleton was achieved using a [3 + 2] disconnection approach.					
34672576	1	49	theme	sponge-derived	222:235	arg1	E					248:248	sponge-derived agminoside E	222:248	sponge-derived agminoside E	222:248	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	5	50	theme	total	974:978	arg1	synthesis					980:988	the total synthesis	970:988	the total synthesis of sponge-derived agminosides	970:1018	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	3	51	theme	donors	630:635	arg1	OTf					503:505	NIS/Yb(OTf)3-	496:508	NIS/Yb(OTf)3-	496:508	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	3	51	theme	donors	630:635	arg1	glycosylations					546:559	TMSOTf-mediated stereoselective glycosylations	514:559	TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors	514:635	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	5	52	from	functions	1062:1070	arg1	sponges					1075:1081	sponges	1075:1081	sponges	1075:1081	Our study lays the foundation for the total synthesis of sponge-derived agminosides and the understanding of their biological functions in sponges.					
34672576	1	53	theme	total	131:135	arg1	synthesis					137:145	the total synthesis	127:145	the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids	127:315	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	1	54	theme	agminoside	237:246	arg1	E					248:248	sponge-derived agminoside E	222:248	sponge-derived agminoside E	222:248	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	0	55	theme	Acetylated	63:72	arg1	Backbone					74:81	the Partially Acetylated Backbone	49:81	the Partially Acetylated Backbone of Sponge-Derived Agminoside E	49:112	Total Synthesis of a Chimeric Glycolipid Bearing the Partially Acetylated Backbone of Sponge-Derived Agminoside E.					
34672576	3	56	theme	glycosylations	546:559	arg1	combination					481:491	a combination	479:491	a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors	479:635	The β-(1 → 2) and β-(1 → 4)-glycosidic bonds were synthesized through a combination of NIS/Yb(OTf)3- and TMSOTf-mediated stereoselective glycosylations of thiotolyl, N-phenyltrifluoroacetimidate, and trichloroacetimidate donors.					
34672576	4	57	theme	Staudinger	667:676	arg1	reduction					678:686	Staudinger reduction	667:686	Staudinger reduction of a (2-azidomethyl)benzoyl group	667:720	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	4	57	theme	Staudinger	667:676	arg1	pentaacetylation					649:664	Late-stage pentaacetylation	638:664	Late-stage pentaacetylation	638:664	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34672576	1	58	theme	E	248:248	arg1	chain					285:289	the (R)-3-hydroxydecanoic acid chain	254:289	the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids	254:315	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	1	58	theme	E	248:248	arg1	backbone					210:217	the partially acetylated backbone	185:217	the partially acetylated backbone of sponge-derived agminoside E	185:248	We describe the total synthesis of a chimeric glycolipid bearing both the partially acetylated backbone of sponge-derived agminoside E and the (R)-3-hydroxydecanoic acid chain of bacterial rhamnolipids.					
34672576	4	59	theme	total	793:797	arg1	synthesis					799:807	the total synthesis	789:807	the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E	789:933	Late-stage pentaacetylation, Staudinger reduction of a (2-azidomethyl)benzoyl group, followed by continuous-flow microfluidic hydrogenolysis completed the total synthesis of the structurally simplified glycolipid, whose partial acetylation pattern on the glycan part was identical to agminoside E.					
34015330	4	0	theme	ribitol	490:496	arg1	phosphate					498:506	ribitol phosphate	490:506	ribitol phosphate	490:506	Enzymes in the Golgi apparatus use CDP-ribitol to incorporate ribitol phosphate into the glycan chain of α-dystroglycan.					
34015330	11	1	theme	nucleotide	1596:1605	arg1	conjugates					1607:1616	cytosine nucleotide conjugates	1587:1616	cytosine nucleotide conjugates	1587:1616	This demonstrates that the size of the binding pocket determines the substrate specificity of SLC35A1, allowing a variety of cytosine nucleotide conjugates to be transported.					
34015330	1	2	theme	intracellular	149:161	arg1	cytoskeleton					163:174	the intracellular cytoskeleton	145:174	the intracellular cytoskeleton	145:174	The glycoprotein α-dystroglycan helps to link the intracellular cytoskeleton to the extracellular matrix.					
34015330	7	3	theme	bulky	892:896	arg1	residues					898:905	bulky residues	892:905	bulky residues in the predicted binding pocket of SLC35A4	892:948	These two transporters are closely related, but bulky residues in the predicted binding pocket of SLC35A4 limit its size.					
34015330	0	4	theme	CDP-ribitol	73:83	arg1	transport					60:68	redundant transport	50:68	redundant transport of CDP-ribitol to the Golgi	50:96	The promiscuous binding pocket of SLC35A1 ensures redundant transport of CDP-ribitol to the Golgi.					
34015330	11	5	theme	conjugates	1607:1616	arg1	variety					1576:1582	a variety	1574:1582	a variety of cytosine nucleotide conjugates	1574:1616	This demonstrates that the size of the binding pocket determines the substrate specificity of SLC35A1, allowing a variety of cytosine nucleotide conjugates to be transported.					
34015330	11	5	theme	conjugates	1607:1616	arg1	conjugates					1607:1616	cytosine nucleotide conjugates	1587:1616	cytosine nucleotide conjugates	1587:1616	This demonstrates that the size of the binding pocket determines the substrate specificity of SLC35A1, allowing a variety of cytosine nucleotide conjugates to be transported.					
34015330	12	6	with	redundancy	1641:1650	arg1	SLC35A4					1657:1663	SLC35A4	1657:1663	SLC35A4	1657:1663	The redundancy with SLC35A4 also explains why patients with SLC35A1 mutations do not show symptoms of α-dystroglycan deficiency.					
34015330	8	7	theme	large	991:995	arg1	pocket					1005:1010	the large binding pocket	987:1010	the large binding pocket SLC35A1	987:1018	We hypothesized that the large binding pocket SLC35A1 might accommodate the bulky CMP-sialic acid and the smaller CDP-ribitol, whereas SLC35A4 might only accept CDP-ribitol.					
34015330	8	8	theme	bulky	1042:1046	arg1	acid					1059:1062	the bulky CMP-sialic acid	1038:1062	the bulky CMP-sialic acid	1038:1062	We hypothesized that the large binding pocket SLC35A1 might accommodate the bulky CMP-sialic acid and the smaller CDP-ribitol, whereas SLC35A4 might only accept CDP-ribitol.					
34015330	11	9	theme	substrate	1531:1539	arg1	specificity					1541:1551	the substrate specificity	1527:1551	the substrate specificity of SLC35A1	1527:1562	This demonstrates that the size of the binding pocket determines the substrate specificity of SLC35A1, allowing a variety of cytosine nucleotide conjugates to be transported.					
34015330	2	10	theme	extracellular	293:305	arg1	proteins					314:321	extracellular matrix proteins	293:321	extracellular matrix proteins such as laminin	293:337	A unique glycan structure attached to this protein is required for its interaction with extracellular matrix proteins such as laminin.					
34015330	2	10	theme	extracellular	293:305	arg1	laminin					331:337	laminin	331:337	laminin	331:337	A unique glycan structure attached to this protein is required for its interaction with extracellular matrix proteins such as laminin.					
34015330	11	11	theme	SLC35A1	1556:1562	arg1	specificity					1541:1551	the substrate specificity	1527:1551	the substrate specificity of SLC35A1	1527:1562	This demonstrates that the size of the binding pocket determines the substrate specificity of SLC35A1, allowing a variety of cytosine nucleotide conjugates to be transported.					
34015330	10	12	theme	present	1319:1325	arg1	residues					1310:1317	the bulky residues	1300:1317	the bulky residues present in SLC35A4	1300:1336	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	11	13	theme	pocket	1509:1514	arg1	size					1489:1492	the size	1485:1492	the size of the binding pocket	1485:1514	This demonstrates that the size of the binding pocket determines the substrate specificity of SLC35A1, allowing a variety of cytosine nucleotide conjugates to be transported.					
34015330	10	14	from	proteins	1399:1406	arg1	cells					1411:1415	cells	1411:1415	cells	1411:1415	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	2	15	attach	attached	231:238	arg2	structure					221:229	A unique glycan structure	205:229	A unique glycan structure attached to this protein	205:254	A unique glycan structure attached to this protein is required for its interaction with extracellular matrix proteins such as laminin.					
34015330	2	15	attach	attached	231:238	arg1	protein					248:254	this protein	243:254	this protein	243:254	A unique glycan structure attached to this protein is required for its interaction with extracellular matrix proteins such as laminin.					
34015330	1	16	attach	link	140:143	arg1	matrix					197:202	the extracellular matrix	179:202	the extracellular matrix	179:202	The glycoprotein α-dystroglycan helps to link the intracellular cytoskeleton to the extracellular matrix.					
34015330	1	16	attach	link	140:143	arg2	α-dystroglycan					116:129	The glycoprotein α-dystroglycan	99:129	The glycoprotein α-dystroglycan	99:129	The glycoprotein α-dystroglycan helps to link the intracellular cytoskeleton to the extracellular matrix.					
34015330	6	17	theme	transporter	801:811	arg1	SLC35A4					813:819	the transporter SLC35A4	797:819	the transporter SLC35A4 in a redundant manner	797:841	We discovered that CDP-ribitol transport relies on the CMP-sialic acid transporter SLC35A1 and the transporter SLC35A4 in a redundant manner.					
34015330	1	18	theme	extracellular	183:195	arg1	matrix					197:202	the extracellular matrix	179:202	the extracellular matrix	179:202	The glycoprotein α-dystroglycan helps to link the intracellular cytoskeleton to the extracellular matrix.					
34015330	6	19	from	SLC35A4	813:819	arg1	manner					836:841	a redundant manner	824:841	a redundant manner	824:841	We discovered that CDP-ribitol transport relies on the CMP-sialic acid transporter SLC35A1 and the transporter SLC35A4 in a redundant manner.					
34015330	5	20	theme	unknown	624:630	arg1	transporter					632:642	an unknown transporter	621:642	an unknown transporter	621:642	Since CDP-ribitol is synthesized in the cytoplasm, we hypothesized that an unknown transporter must be required for its import into the Golgi apparatus.					
34015330	8	21	theme	binding	997:1003	arg1	pocket					1005:1010	the large binding pocket	987:1010	the large binding pocket SLC35A1	987:1018	We hypothesized that the large binding pocket SLC35A1 might accommodate the bulky CMP-sialic acid and the smaller CDP-ribitol, whereas SLC35A4 might only accept CDP-ribitol.					
34015330	10	22	theme	binding	1261:1267	arg1	site					1269:1272	the binding site	1257:1272	the binding site of SLC35A1	1257:1283	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	10	22	theme	binding	1261:1267	arg1	SLC35A1					1277:1283	SLC35A1	1277:1283	SLC35A1	1277:1283	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	9	23	with	SLC35A1	1167:1173	arg1	mutations					1180:1188	mutations	1180:1188	mutations in its binding pocket in SLC35A1 KO cell lines	1180:1235	To test this, we expressed SLC35A1 with mutations in its binding pocket in SLC35A1 KO cell lines.					
34015330	0	24	theme	promiscuous	4:14	arg1	pocket					24:29	The promiscuous binding pocket	0:29	The promiscuous binding pocket of SLC35A1	0:40	The promiscuous binding pocket of SLC35A1 ensures redundant transport of CDP-ribitol to the Golgi.					
34015330	10	25	theme	proteins	1399:1406	arg1	sialylation					1384:1394	sialylation	1384:1394	sialylation of proteins in cells	1384:1415	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	10	26	theme	ribitol	1437:1443	arg1	phosphorylation					1445:1459	ribitol phosphorylation	1437:1459	ribitol phosphorylation	1437:1459	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	7	27	theme	SLC35A4	942:948	arg1	pocket					932:937	the predicted binding pocket	910:937	the predicted binding pocket of SLC35A4	910:948	These two transporters are closely related, but bulky residues in the predicted binding pocket of SLC35A4 limit its size.					
34015330	7	28	from	residues	898:905	arg1	pocket					932:937	the predicted binding pocket	910:937	the predicted binding pocket of SLC35A4	910:948	These two transporters are closely related, but bulky residues in the predicted binding pocket of SLC35A4 limit its size.					
34015330	3	29	theme	phosphate	410:418	arg1	groups					420:425	ribitol phosphate groups	402:425	ribitol phosphate groups	402:425	Up to now, this is the only mammalian glycan known to contain ribitol phosphate groups.					
34015330	10	30	from	cells	1411:1415	arg1	sialylation					1384:1394	sialylation	1384:1394	sialylation of proteins in cells	1384:1415	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	7	31	theme	binding	924:930	arg1	pocket					932:937	the predicted binding pocket	910:937	the predicted binding pocket of SLC35A4	910:948	These two transporters are closely related, but bulky residues in the predicted binding pocket of SLC35A4 limit its size.					
34015330	12	32	with	patients	1683:1690	arg1	mutations					1705:1713	SLC35A1 mutations	1697:1713	SLC35A1 mutations	1697:1713	The redundancy with SLC35A4 also explains why patients with SLC35A1 mutations do not show symptoms of α-dystroglycan deficiency.					
34015330	10	33	from	present	1319:1325	arg1	SLC35A4					1330:1336	SLC35A4	1330:1336	SLC35A4	1330:1336	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	12	34	theme	α-dystroglycan	1739:1752	arg1	deficiency					1754:1763	α-dystroglycan deficiency	1739:1763	α-dystroglycan deficiency	1739:1763	The redundancy with SLC35A4 also explains why patients with SLC35A1 mutations do not show symptoms of α-dystroglycan deficiency.					
34015330	6	35	theme	transporter	773:783	arg1	SLC35A1					785:791	the CMP-sialic acid transporter SLC35A1	753:791	the CMP-sialic acid transporter SLC35A1	753:791	We discovered that CDP-ribitol transport relies on the CMP-sialic acid transporter SLC35A1 and the transporter SLC35A4 in a redundant manner.					
34015330	6	36	from	SLC35A1	785:791	arg1	manner					836:841	a redundant manner	824:841	a redundant manner	824:841	We discovered that CDP-ribitol transport relies on the CMP-sialic acid transporter SLC35A1 and the transporter SLC35A4 in a redundant manner.					
34015330	3	37	theme	only	363:366	arg1	glycan					378:383	the only mammalian glycan	359:383	the only mammalian glycan known to contain ribitol phosphate groups	359:425	Up to now, this is the only mammalian glycan known to contain ribitol phosphate groups.					
34015330	3	37	theme	only	363:366	arg1	this					351:354	this	351:354	this	351:354	Up to now, this is the only mammalian glycan known to contain ribitol phosphate groups.					
34015330	11	38	theme	binding	1501:1507	arg1	pocket					1509:1514	the binding pocket	1497:1514	the binding pocket	1497:1514	This demonstrates that the size of the binding pocket determines the substrate specificity of SLC35A1, allowing a variety of cytosine nucleotide conjugates to be transported.					
34015330	1	39	gly	glycoprotein	103:114	arg1	glycoprotein					103:114	The glycoprotein α-dystroglycan	99:129	The glycoprotein α-dystroglycan	99:129	The glycoprotein α-dystroglycan helps to link the intracellular cytoskeleton to the extracellular matrix.					
34015330	9	40	theme	SLC35A1	1215:1221	arg1	lines					1231:1235	SLC35A1 KO cell lines	1215:1235	SLC35A1 KO cell lines	1215:1235	To test this, we expressed SLC35A1 with mutations in its binding pocket in SLC35A1 KO cell lines.					
34015330	2	41	theme	matrix	307:312	arg1	proteins					314:321	extracellular matrix proteins	293:321	extracellular matrix proteins such as laminin	293:337	A unique glycan structure attached to this protein is required for its interaction with extracellular matrix proteins such as laminin.					
34015330	2	41	theme	matrix	307:312	arg1	laminin					331:337	laminin	331:337	laminin	331:337	A unique glycan structure attached to this protein is required for its interaction with extracellular matrix proteins such as laminin.					
34015330	10	42	gly	sialylation	1384:1394	arg1	proteins					1399:1406	proteins	1399:1406	proteins in cells	1399:1415	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	0	43	theme	SLC35A1	34:40	arg1	pocket					24:29	The promiscuous binding pocket	0:29	The promiscuous binding pocket of SLC35A1	0:40	The promiscuous binding pocket of SLC35A1 ensures redundant transport of CDP-ribitol to the Golgi.					
34015330	10	44	from	SLC35A4	1330:1336	arg1	present					1319:1325	present	1319:1325	present	1319:1325	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	10	45	from	sialylation	1384:1394	arg1	cells					1411:1415	cells	1411:1415	cells	1411:1415	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	9	46	theme	KO	1223:1224	arg1	lines					1231:1235	SLC35A1 KO cell lines	1215:1235	SLC35A1 KO cell lines	1215:1235	To test this, we expressed SLC35A1 with mutations in its binding pocket in SLC35A1 KO cell lines.					
34015330	8	47	theme	smaller	1072:1078	arg1	CDP-ribitol					1080:1090	the smaller CDP-ribitol	1068:1090	the smaller CDP-ribitol	1068:1090	We hypothesized that the large binding pocket SLC35A1 might accommodate the bulky CMP-sialic acid and the smaller CDP-ribitol, whereas SLC35A4 might only accept CDP-ribitol.					
34015330	10	48	theme	SLC35A1	1277:1283	arg1	site					1269:1272	the binding site	1257:1272	the binding site of SLC35A1	1257:1283	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	10	48	theme	SLC35A1	1277:1283	arg1	SLC35A1					1277:1283	SLC35A1	1277:1283	SLC35A1	1277:1283	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	3	49	theme	ribitol	402:408	arg1	groups					420:425	ribitol phosphate groups	402:425	ribitol phosphate groups	402:425	Up to now, this is the only mammalian glycan known to contain ribitol phosphate groups.					
34015330	10	50	attach	present	1319:1325	arg1	SLC35A4					1330:1336	SLC35A4	1330:1336	SLC35A4	1330:1336	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	10	50	attach	present	1319:1325	arg2	residues					1310:1317	the bulky residues	1300:1317	the bulky residues present in SLC35A4	1300:1336	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	9	51	theme	cell	1226:1229	arg1	lines					1231:1235	SLC35A1 KO cell lines	1215:1235	SLC35A1 KO cell lines	1215:1235	To test this, we expressed SLC35A1 with mutations in its binding pocket in SLC35A1 KO cell lines.					
34015330	6	52	theme	acid	768:771	arg1	SLC35A1					785:791	the CMP-sialic acid transporter SLC35A1	753:791	the CMP-sialic acid transporter SLC35A1	753:791	We discovered that CDP-ribitol transport relies on the CMP-sialic acid transporter SLC35A1 and the transporter SLC35A4 in a redundant manner.					
34015330	11	53	theme	cytosine	1587:1594	arg1	conjugates					1607:1616	cytosine nucleotide conjugates	1587:1616	cytosine nucleotide conjugates	1587:1616	This demonstrates that the size of the binding pocket determines the substrate specificity of SLC35A1, allowing a variety of cytosine nucleotide conjugates to be transported.					
34015330	0	54	theme	redundant	50:58	arg1	transport					60:68	redundant transport	50:68	redundant transport of CDP-ribitol to the Golgi	50:96	The promiscuous binding pocket of SLC35A1 ensures redundant transport of CDP-ribitol to the Golgi.					
34015330	4	55	theme	glycan	517:522	arg1	chain					524:528	the glycan chain	513:528	the glycan chain of α-dystroglycan	513:546	Enzymes in the Golgi apparatus use CDP-ribitol to incorporate ribitol phosphate into the glycan chain of α-dystroglycan.					
34015330	4	56	from	Enzymes	428:434	arg1	apparatus					449:457	the Golgi apparatus	439:457	the Golgi apparatus	439:457	Enzymes in the Golgi apparatus use CDP-ribitol to incorporate ribitol phosphate into the glycan chain of α-dystroglycan.					
34015330	4	57	theme	Golgi	443:447	arg1	apparatus					449:457	the Golgi apparatus	439:457	the Golgi apparatus	439:457	Enzymes in the Golgi apparatus use CDP-ribitol to incorporate ribitol phosphate into the glycan chain of α-dystroglycan.					
34015330	6	58	theme	CMP-sialic	757:766	arg1	SLC35A1					785:791	the CMP-sialic acid transporter SLC35A1	753:791	the CMP-sialic acid transporter SLC35A1	753:791	We discovered that CDP-ribitol transport relies on the CMP-sialic acid transporter SLC35A1 and the transporter SLC35A4 in a redundant manner.					
34015330	1	59	theme	glycoprotein	103:114	arg1	α-dystroglycan					116:129	The glycoprotein α-dystroglycan	99:129	The glycoprotein α-dystroglycan	99:129	The glycoprotein α-dystroglycan helps to link the intracellular cytoskeleton to the extracellular matrix.					
34015330	4	60	theme	α-dystroglycan	533:546	arg1	chain					524:528	the glycan chain	513:528	the glycan chain of α-dystroglycan	513:546	Enzymes in the Golgi apparatus use CDP-ribitol to incorporate ribitol phosphate into the glycan chain of α-dystroglycan.					
34015330	2	61	theme	glycan	214:219	arg1	structure					221:229	A unique glycan structure	205:229	A unique glycan structure attached to this protein	205:254	A unique glycan structure attached to this protein is required for its interaction with extracellular matrix proteins such as laminin.					
34015330	8	62	theme	CMP-sialic	1048:1057	arg1	acid					1059:1062	the bulky CMP-sialic acid	1038:1062	the bulky CMP-sialic acid	1038:1062	We hypothesized that the large binding pocket SLC35A1 might accommodate the bulky CMP-sialic acid and the smaller CDP-ribitol, whereas SLC35A4 might only accept CDP-ribitol.					
34015330	8	63	theme	pocket	1005:1010	arg1	SLC35A1					1012:1018	the large binding pocket SLC35A1	987:1018	the large binding pocket SLC35A1	987:1018	We hypothesized that the large binding pocket SLC35A1 might accommodate the bulky CMP-sialic acid and the smaller CDP-ribitol, whereas SLC35A4 might only accept CDP-ribitol.					
34015330	9	64	from	pocket	1205:1210	arg1	lines					1231:1235	SLC35A1 KO cell lines	1215:1235	SLC35A1 KO cell lines	1215:1235	To test this, we expressed SLC35A1 with mutations in its binding pocket in SLC35A1 KO cell lines.					
34015330	2	65	theme	unique	207:212	arg1	structure					221:229	A unique glycan structure	205:229	A unique glycan structure attached to this protein	205:254	A unique glycan structure attached to this protein is required for its interaction with extracellular matrix proteins such as laminin.					
34015330	10	66	theme	bulky	1304:1308	arg1	residues					1310:1317	the bulky residues	1300:1317	the bulky residues present in SLC35A4	1300:1336	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	7	67	theme	predicted	914:922	arg1	pocket					932:937	the predicted binding pocket	910:937	the predicted binding pocket of SLC35A4	910:948	These two transporters are closely related, but bulky residues in the predicted binding pocket of SLC35A4 limit its size.					
34015330	2	68	with	interaction	276:286	arg1	proteins					314:321	extracellular matrix proteins	293:321	extracellular matrix proteins such as laminin	293:337	A unique glycan structure attached to this protein is required for its interaction with extracellular matrix proteins such as laminin.					
34015330	2	68	with	interaction	276:286	arg1	laminin					331:337	laminin	331:337	laminin	331:337	A unique glycan structure attached to this protein is required for its interaction with extracellular matrix proteins such as laminin.					
34015330	5	69	theme	Golgi	685:689	arg1	apparatus					691:699	the Golgi apparatus	681:699	the Golgi apparatus	681:699	Since CDP-ribitol is synthesized in the cytoplasm, we hypothesized that an unknown transporter must be required for its import into the Golgi apparatus.					
34015330	10	70	theme	mutant	1343:1348	arg1	unable					1366:1371	unable	1366:1371	unable	1366:1371	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	10	70	theme	mutant	1343:1348	arg1	transporter					1350:1360	the mutant transporter	1339:1360	the mutant transporter	1339:1360	When we restricted the binding site of SLC35A1 by introducing the bulky residues present in SLC35A4, the mutant transporter was unable to support sialylation of proteins in cells but still supported ribitol phosphorylation.					
34015330	6	71	theme	redundant	826:834	arg1	manner					836:841	a redundant manner	824:841	a redundant manner	824:841	We discovered that CDP-ribitol transport relies on the CMP-sialic acid transporter SLC35A1 and the transporter SLC35A4 in a redundant manner.					
34015330	12	72	theme	deficiency	1754:1763	arg1	symptoms					1727:1734	symptoms	1727:1734	symptoms of α-dystroglycan deficiency	1727:1763	The redundancy with SLC35A4 also explains why patients with SLC35A1 mutations do not show symptoms of α-dystroglycan deficiency.					
34015330	9	73	from	mutations	1180:1188	arg1	pocket					1205:1210	its binding pocket	1193:1210	its binding pocket in SLC35A1 KO cell lines	1193:1235	To test this, we expressed SLC35A1 with mutations in its binding pocket in SLC35A1 KO cell lines.					
34015330	12	74	theme	SLC35A1	1697:1703	arg1	mutations					1705:1713	SLC35A1 mutations	1697:1713	SLC35A1 mutations	1697:1713	The redundancy with SLC35A4 also explains why patients with SLC35A1 mutations do not show symptoms of α-dystroglycan deficiency.					
34015330	9	75	theme	binding	1197:1203	arg1	pocket					1205:1210	its binding pocket	1193:1210	its binding pocket in SLC35A1 KO cell lines	1193:1235	To test this, we expressed SLC35A1 with mutations in its binding pocket in SLC35A1 KO cell lines.					
34015330	0	76	theme	binding	16:22	arg1	pocket					24:29	The promiscuous binding pocket	0:29	The promiscuous binding pocket of SLC35A1	0:40	The promiscuous binding pocket of SLC35A1 ensures redundant transport of CDP-ribitol to the Golgi.					
34015330	6	77	theme	CDP-ribitol	721:731	arg1	transport					733:741	CDP-ribitol transport	721:741	CDP-ribitol transport	721:741	We discovered that CDP-ribitol transport relies on the CMP-sialic acid transporter SLC35A1 and the transporter SLC35A4 in a redundant manner.					
34015330	3	78	theme	mammalian	368:376	arg1	glycan					378:383	the only mammalian glycan	359:383	the only mammalian glycan known to contain ribitol phosphate groups	359:425	Up to now, this is the only mammalian glycan known to contain ribitol phosphate groups.					
34015330	3	78	theme	mammalian	368:376	arg1	this					351:354	this	351:354	this	351:354	Up to now, this is the only mammalian glycan known to contain ribitol phosphate groups.					
33878161	5	0	theme	lysosomal	757:765	arg1	lipase					772:777	lysosomal acid lipase	757:777	lysosomal acid lipase	757:777	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	0	theme	lysosomal	757:765	arg1	proteins					747:754	Two recombinant proteins	731:754	Two recombinant proteins	731:754	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	8	1	theme	human	1211:1215	arg1	tumours					1217:1223	human tumours	1211:1223	human tumours	1211:1223	Moreover, KO cell lines would be useful as models for researching the mechanism of antimetastatic effects in human tumours by swainsonine treatment.					
33878161	5	2	theme	recombinant	735:745	arg1	lipase					772:777	lysosomal acid lipase	757:777	lysosomal acid lipase	757:777	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	2	theme	recombinant	735:745	arg1	fragment					792:799	constant fragment	783:799	constant fragment of human IgG1	783:813	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	2	theme	recombinant	735:745	arg1	proteins					747:754	Two recombinant proteins	731:754	Two recombinant proteins	731:754	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	3	theme	acid	767:770	arg1	lipase					772:777	lysosomal acid lipase	757:777	lysosomal acid lipase	757:777	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	3	theme	acid	767:770	arg1	proteins					747:754	Two recombinant proteins	731:754	Two recombinant proteins	731:754	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	3	4	theme	recombinant	485:495	arg1	proteins					497:504	recombinant proteins	485:504	recombinant proteins mainly with hybrid-type N-glycans	485:538	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	3	5	with	proteins	497:504	arg1	N-glycans					530:538	hybrid-type N-glycans	518:538	hybrid-type N-glycans	518:538	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	6	6	theme	structural	874:883	arg1	analysis					885:892	Glycan structural analysis	867:892	Glycan structural analysis	867:892	Glycan structural analysis revealed that complex-type N-glycans were removed in both M2D-KO and DF-KO cells.					
33878161	2	7	gly	heterogeneity	174:186	arg1	glycans					191:197	glycans	191:197	glycans	191:197	The heterogeneity of glycans in therapeutic proteins is an issue for maintaining quality, activity and safety during bioprocessing.					
33878161	3	8	from	MAN2A1	371:376	arg1	cells					428:432	human embryonic kidney 293 (HEK293) cells	392:432	human embryonic kidney 293 (HEK293) cells	392:432	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	4	9	theme	fucosylated	696:706	arg1	N-glycans					720:728	noncore fucosylated hybrid-type N-glycans	688:728	noncore fucosylated hybrid-type N-glycans	688:728	Furthermore, FUT8, which encodes α1,6-fucosyltransferase, was knocked out in the M2D-KO cell line, establishing a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans.					
33878161	6	10	theme	Glycan	867:872	arg1	analysis					885:892	Glycan structural analysis	867:892	Glycan structural analysis	867:892	Glycan structural analysis revealed that complex-type N-glycans were removed in both M2D-KO and DF-KO cells.					
33878161	7	11	theme	hybrid-type	1079:1089	arg1	N-glycans					1091:1099	hybrid-type N-glycans	1079:1099	hybrid-type N-glycans	1079:1099	Our results suggest that these cell lines are suitable for the production of therapeutic proteins with hybrid-type N-glycans.					
33878161	5	12	theme	DF-KO	849:853	arg1	lines					860:864	the M2D-KO and DF-KO cell lines	834:864	lines	860:864	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	3	13	theme	Golgi	347:351	arg1	α-mannosidase-II					353:368	Golgi α-mannosidase-II	347:368	Golgi α-mannosidase-II	347:368	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	3	14	from	MAN2A2	382:387	arg1	cells					428:432	human embryonic kidney 293 (HEK293) cells	392:432	human embryonic kidney 293 (HEK293) cells	392:432	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	8	15	theme	effects	1200:1206	arg1	mechanism					1172:1180	the mechanism	1168:1180	the mechanism of antimetastatic effects in human tumours	1168:1223	Moreover, KO cell lines would be useful as models for researching the mechanism of antimetastatic effects in human tumours by swainsonine treatment.					
33878161	1	16	from	products	126:133	arg1	industry					160:167	the biopharmaceutical industry	138:167	the biopharmaceutical industry	138:167	Glycoprotein therapeutics are among the leading products in the biopharmaceutical industry.					
33878161	3	17	theme	hybrid-type	518:528	arg1	N-glycans					530:538	hybrid-type N-glycans	518:538	hybrid-type N-glycans	518:538	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	0	18	from	production	25:34	arg1	cells					71:75	HEK293 cells	64:75	HEK293 cells	64:75	Cell engineering for the production of hybrid-type N-glycans in HEK293 cells.					
33878161	7	19	theme	proteins	1065:1072	arg1	production					1039:1048	the production	1035:1048	the production of therapeutic proteins with hybrid-type N-glycans	1035:1099	Our results suggest that these cell lines are suitable for the production of therapeutic proteins with hybrid-type N-glycans.					
33878161	0	20	theme	HEK293	64:69	arg1	cells					71:75	HEK293 cells	64:75	HEK293 cells	64:75	Cell engineering for the production of hybrid-type N-glycans in HEK293 cells.					
33878161	1	21	theme	Glycoprotein	78:89	arg1	therapeutics					91:102	Glycoprotein therapeutics	78:102	Glycoprotein therapeutics	78:102	Glycoprotein therapeutics are among the leading products in the biopharmaceutical industry.					
33878161	4	22	theme	DF-KO	655:659	arg1	line					666:669	a DF-KO cell line	653:669	a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans	653:728	Furthermore, FUT8, which encodes α1,6-fucosyltransferase, was knocked out in the M2D-KO cell line, establishing a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans.					
33878161	0	23	theme	Cell	0:3	arg1	engineering					5:15	Cell engineering	0:15	Cell engineering for the production of hybrid-type N-glycans in HEK293 cells	0:75	Cell engineering for the production of hybrid-type N-glycans in HEK293 cells.					
33878161	6	24	theme	DF-KO	963:967	arg1	cells					969:973	DF-KO cells	963:973	DF-KO cells	963:973	Glycan structural analysis revealed that complex-type N-glycans were removed in both M2D-KO and DF-KO cells.					
33878161	0	25	theme	hybrid-type	39:49	arg1	N-glycans					51:59	hybrid-type N-glycans	39:59	hybrid-type N-glycans	39:59	Cell engineering for the production of hybrid-type N-glycans in HEK293 cells.					
33878161	5	26	theme	cell	855:858	arg1	lines					860:864	the M2D-KO and DF-KO cell lines	834:864	lines	860:864	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	27	theme	IgG1	810:813	arg1	lipase					772:777	lysosomal acid lipase	757:777	lysosomal acid lipase	757:777	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	27	theme	IgG1	810:813	arg1	fragment					792:799	constant fragment	783:799	constant fragment of human IgG1	783:813	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	27	theme	IgG1	810:813	arg1	proteins					747:754	Two recombinant proteins	731:754	Two recombinant proteins	731:754	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	28	theme	human	804:808	arg1	IgG1					810:813	human IgG1	804:813	human IgG1	804:813	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	29	theme	constant	783:790	arg1	fragment					792:799	constant fragment	783:799	constant fragment of human IgG1	783:813	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	5	29	theme	constant	783:790	arg1	proteins					747:754	Two recombinant proteins	731:754	Two recombinant proteins	731:754	Two recombinant proteins, lysosomal acid lipase and constant fragment of human IgG1, were expressed in the M2D-KO and DF-KO cell lines.					
33878161	4	30	theme	M2D-KO	622:627	arg1	line					634:637	the M2D-KO cell line	618:637	the M2D-KO cell line	618:637	Furthermore, FUT8, which encodes α1,6-fucosyltransferase, was knocked out in the M2D-KO cell line, establishing a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans.					
33878161	7	31	theme	therapeutic	1053:1063	arg1	proteins					1065:1072	therapeutic proteins	1053:1072	therapeutic proteins with hybrid-type N-glycans	1053:1099	Our results suggest that these cell lines are suitable for the production of therapeutic proteins with hybrid-type N-glycans.					
33878161	2	32	theme	glycans	191:197	arg1	heterogeneity					174:186	The heterogeneity	170:186	The heterogeneity of glycans in therapeutic proteins	170:221	The heterogeneity of glycans in therapeutic proteins is an issue for maintaining quality, activity and safety during bioprocessing.					
33878161	2	32	theme	glycans	191:197	arg1	issue					229:233	an issue	226:233	an issue for maintaining quality, activity and safety during bioprocessing	226:299	The heterogeneity of glycans in therapeutic proteins is an issue for maintaining quality, activity and safety during bioprocessing.					
33878161	8	33	from	mechanism	1172:1180	arg1	tumours					1217:1223	human tumours	1211:1223	human tumours	1211:1223	Moreover, KO cell lines would be useful as models for researching the mechanism of antimetastatic effects in human tumours by swainsonine treatment.					
33878161	3	34	theme	M2D-KO	451:456	arg1	line					463:466	an M2D-KO cell line	448:466	an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans	448:538	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	3	35	theme	kidney	408:413	arg1	cells					428:432	human embryonic kidney 293 (HEK293) cells	392:432	human embryonic kidney 293 (HEK293) cells	392:432	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	8	36	from	effects	1200:1206	arg1	tumours					1217:1223	human tumours	1211:1223	human tumours	1211:1223	Moreover, KO cell lines would be useful as models for researching the mechanism of antimetastatic effects in human tumours by swainsonine treatment.					
33878161	4	37	gly	fucosylated	696:706	arg1	N-glycans					720:728	noncore fucosylated hybrid-type N-glycans	688:728	noncore fucosylated hybrid-type N-glycans	688:728	Furthermore, FUT8, which encodes α1,6-fucosyltransferase, was knocked out in the M2D-KO cell line, establishing a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans.					
33878161	8	38	theme	swainsonine	1228:1238	arg1	treatment					1240:1248	swainsonine treatment	1228:1248	swainsonine treatment	1228:1248	Moreover, KO cell lines would be useful as models for researching the mechanism of antimetastatic effects in human tumours by swainsonine treatment.					
33878161	8	39	theme	antimetastatic	1185:1198	arg1	effects					1200:1206	antimetastatic effects	1185:1206	antimetastatic effects in human tumours	1185:1223	Moreover, KO cell lines would be useful as models for researching the mechanism of antimetastatic effects in human tumours by swainsonine treatment.					
33878161	3	40	from	α-mannosidase-II	353:368	arg1	cells					428:432	human embryonic kidney 293 (HEK293) cells	392:432	human embryonic kidney 293 (HEK293) cells	392:432	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	4	41	theme	cell	629:632	arg1	line					634:637	the M2D-KO cell line	618:637	the M2D-KO cell line	618:637	Furthermore, FUT8, which encodes α1,6-fucosyltransferase, was knocked out in the M2D-KO cell line, establishing a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans.					
33878161	0	42	theme	N-glycans	51:59	arg1	production					25:34	the production	21:34	the production of hybrid-type N-glycans in HEK293 cells	21:75	Cell engineering for the production of hybrid-type N-glycans in HEK293 cells.					
33878161	8	43	theme	cell	1115:1118	arg1	lines					1120:1124	KO cell lines	1112:1124	KO cell lines	1112:1124	Moreover, KO cell lines would be useful as models for researching the mechanism of antimetastatic effects in human tumours by swainsonine treatment.					
33878161	7	44	theme	cell	1007:1010	arg1	lines					1012:1016	these cell lines	1001:1016	these cell lines	1001:1016	Our results suggest that these cell lines are suitable for the production of therapeutic proteins with hybrid-type N-glycans.					
33878161	4	45	theme	noncore	688:694	arg1	N-glycans					720:728	noncore fucosylated hybrid-type N-glycans	688:728	noncore fucosylated hybrid-type N-glycans	688:728	Furthermore, FUT8, which encodes α1,6-fucosyltransferase, was knocked out in the M2D-KO cell line, establishing a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans.					
33878161	3	46	theme	embryonic	398:406	arg1	kidney					408:413	human embryonic kidney 293	392:417	human embryonic kidney 293 (HEK293) cells	392:432	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	3	46	theme	embryonic	398:406	arg1	HEK293					420:425	HEK293	420:425	HEK293	420:425	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	1	47	theme	leading	118:124	arg1	products					126:133	the leading products	114:133	the leading products in the biopharmaceutical industry	114:167	Glycoprotein therapeutics are among the leading products in the biopharmaceutical industry.					
33878161	8	48	theme	KO	1112:1113	arg1	lines					1120:1124	KO cell lines	1112:1124	KO cell lines	1112:1124	Moreover, KO cell lines would be useful as models for researching the mechanism of antimetastatic effects in human tumours by swainsonine treatment.					
33878161	8	49	from	tumours	1217:1223	arg1	mechanism					1172:1180	the mechanism	1168:1180	the mechanism of antimetastatic effects in human tumours	1168:1223	Moreover, KO cell lines would be useful as models for researching the mechanism of antimetastatic effects in human tumours by swainsonine treatment.					
33878161	3	50	theme	cell	458:461	arg1	line					463:466	an M2D-KO cell line	448:466	an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans	448:538	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	2	51	theme	therapeutic	202:212	arg1	proteins					214:221	therapeutic proteins	202:221	therapeutic proteins	202:221	The heterogeneity of glycans in therapeutic proteins is an issue for maintaining quality, activity and safety during bioprocessing.					
33878161	4	52	theme	cell	661:664	arg1	line					666:669	a DF-KO cell line	653:669	a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans	653:728	Furthermore, FUT8, which encodes α1,6-fucosyltransferase, was knocked out in the M2D-KO cell line, establishing a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans.					
33878161	7	53	with	proteins	1065:1072	arg1	N-glycans					1091:1099	hybrid-type N-glycans	1079:1099	hybrid-type N-glycans	1079:1099	Our results suggest that these cell lines are suitable for the production of therapeutic proteins with hybrid-type N-glycans.					
33878161	4	54	theme	hybrid-type	708:718	arg1	N-glycans					720:728	noncore fucosylated hybrid-type N-glycans	688:728	noncore fucosylated hybrid-type N-glycans	688:728	Furthermore, FUT8, which encodes α1,6-fucosyltransferase, was knocked out in the M2D-KO cell line, establishing a DF-KO cell line that can express noncore fucosylated hybrid-type N-glycans.					
33878161	2	55	from	heterogeneity	174:186	arg1	proteins					214:221	therapeutic proteins	202:221	therapeutic proteins	202:221	The heterogeneity of glycans in therapeutic proteins is an issue for maintaining quality, activity and safety during bioprocessing.					
33878161	3	56	theme	human	392:396	arg1	kidney					408:413	human embryonic kidney 293	392:417	human embryonic kidney 293 (HEK293) cells	392:432	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	3	56	theme	human	392:396	arg1	HEK293					420:425	HEK293	420:425	HEK293	420:425	In this study, we knocked out genes encoding Golgi α-mannosidase-II, MAN2A1 and MAN2A2 in human embryonic kidney 293 (HEK293) cells, establishing an M2D-KO cell line that can produce recombinant proteins mainly with hybrid-type N-glycans.					
33878161	6	57	theme	complex-type	908:919	arg1	N-glycans					921:929	complex-type N-glycans	908:929	complex-type N-glycans	908:929	Glycan structural analysis revealed that complex-type N-glycans were removed in both M2D-KO and DF-KO cells.					
33878161	1	58	theme	biopharmaceutical	142:158	arg1	industry					160:167	the biopharmaceutical industry	138:167	the biopharmaceutical industry	138:167	Glycoprotein therapeutics are among the leading products in the biopharmaceutical industry.					
34813787	6	0	theme	1→	916:917	arg1	1→					953:954	→2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	888:954	1→	953:954	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	8	1	dep	repeats	1263:1269	arg1	repeats					1263:1269	type B3S ulvanobiuronic acid repeats	1234:1269	type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1234:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	1	dep	repeats	1263:1269	arg1	ulvanobiose					1312:1322	ulvanobiose	1312:1322	ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1312:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	1	dep	repeats	1263:1269	arg1	-α-L-Rhap3S-					1291:1302	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	1	dep	repeats	1263:1269	arg1	-α-L-Rhap3S-					1348:1359	U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]	1325:1363	U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]	1325:1363	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	9	2	from	species	1506:1512	arg1	ulvans					1488:1493	the ulvans	1484:1493	the ulvans from these species	1484:1512	NMR spectra of U. tepida and U. prolifera showed resonances not detected in U. ohnoi, highlighting the complexity of the ulvans from these species.					
34813787	9	2	from	species	1506:1512	arg1	complexity					1470:1479	the complexity	1466:1479	the complexity of the ulvans from these species	1466:1512	NMR spectra of U. tepida and U. prolifera showed resonances not detected in U. ohnoi, highlighting the complexity of the ulvans from these species.					
34813787	3	3	contain	contained	515:523	arg2	%					548:548	4.4-26.0 mol%	536:548	4.4-26.0 mol%	536:548	In addition, the ulvan samples also contained galactose (4.4-26.0 mol%).					
34813787	3	3	contain	contained	515:523	arg1	addition					482:489	addition	482:489	addition	482:489	In addition, the ulvan samples also contained galactose (4.4-26.0 mol%).					
34813787	3	3	contain	contained	515:523	arg1	samples					502:508	the ulvan samples	492:508	the ulvan samples	492:508	In addition, the ulvan samples also contained galactose (4.4-26.0 mol%).					
34813787	3	3	contain	contained	515:523	arg2	samples					502:508	the ulvan samples	492:508	the ulvan samples	492:508	In addition, the ulvan samples also contained galactose (4.4-26.0 mol%).					
34813787	3	3	contain	contained	515:523	arg2	galactose					525:533	galactose	525:533	galactose (4.4-26.0 mol%)	525:549	In addition, the ulvan samples also contained galactose (4.4-26.0 mol%).					
34813787	1	4	theme	acidic	230:235	arg1	conditions					237:246	mild acidic conditions	225:246	mild acidic conditions	225:246	Ulvans from Ulva ohnoi, Ulva tepida and Ulva prolifera were extracted under mild acidic conditions, isolated and their composition and structure determined.					
34813787	0	5	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of ulvans	0:36	Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva.					
34813787	8	6	theme	nuclear	1036:1042	arg1	resonance					1053:1061	nuclear magnetic resonance	1036:1061	nuclear magnetic resonance (NMR) spectroscopy	1036:1080	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	6	theme	nuclear	1036:1042	arg1	NMR					1064:1066	NMR	1064:1066	NMR	1064:1066	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	7	theme	resonance	1053:1061	arg1	spectroscopy					1069:1080	nuclear magnetic resonance (NMR) spectroscopy	1036:1080	nuclear magnetic resonance (NMR) spectroscopy	1036:1080	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	4	8	theme	linkage	561:567	arg1	analysis					569:576	Glycosyl linkage analysis	552:576	Glycosyl linkage analysis	552:576	Glycosyl linkage analysis showed that ulvan from U. ohnoi contained mostly →4)-GlcpA-(1→ and →3,4)-Rhap-(1→.					
34813787	5	9	theme	standards	713:721	arg1	Preparation					661:671	Preparation	661:671	Preparation of partially methylated alditol acetate standards of idose	661:730	Preparation of partially methylated alditol acetate standards of idose showed that U. ohnoi contained →4)-IdopA-(1→.					
34813787	5	10	theme	U.	744:745	arg1	ohnoi					747:751	U. ohnoi	744:751	U. ohnoi	744:751	Preparation of partially methylated alditol acetate standards of idose showed that U. ohnoi contained →4)-IdopA-(1→.					
34813787	6	11	theme	glycosyl	809:816	arg1	analysis					826:833	glycosyl linkage analysis	809:833	glycosyl linkage analysis of U. tepida and U. prolifera	809:863	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	9	12	theme	prolifera	1399:1407	arg1	spectra					1371:1377	NMR spectra	1367:1377	NMR spectra of U. tepida and U. prolifera	1367:1407	NMR spectra of U. tepida and U. prolifera showed resonances not detected in U. ohnoi, highlighting the complexity of the ulvans from these species.					
34813787	1	13	from	ohnoi	166:170	arg1	Ulvans					149:154	Ulvans	149:154	Ulvans from Ulva ohnoi, Ulva tepida and Ulva prolifera	149:202	Ulvans from Ulva ohnoi, Ulva tepida and Ulva prolifera were extracted under mild acidic conditions, isolated and their composition and structure determined.					
34813787	8	14	dep	together	1022:1029	arg1	with					1031:1034	with	1031:1034	with	1031:1034	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	0	15	dep	species	121:127	arg1	prolifera					110:118	Ulva prolifera	105:118	Ulva prolifera	105:118	Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva.					
34813787	0	15	dep	species	121:127	arg1	tepida					94:99	Ulva tepida	89:99	Ulva tepida	89:99	Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva.					
34813787	8	16	theme	type	1234:1237	arg1	repeats					1263:1269	type B3S ulvanobiuronic acid repeats	1234:1269	type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1234:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	16	theme	type	1234:1237	arg1	ulvanobiose					1312:1322	ulvanobiose	1312:1322	ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1312:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	16	theme	type	1234:1237	arg1	-α-L-Rhap3S-					1291:1302	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	17	dep	-α-L-Rhap3S-	1348:1359	arg1	1→4					1344:1346	1→4	1344:1346	1→4	1344:1346	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	10	18	located	detected	1632:1639	arg2	activity					1623:1630	inhibitory activity	1612:1630	inhibitory activity	1612:1630	Regardless of the structural diversity of the ulvan samples there was very little antioxidant or inhibitory activity detected on enzymatic processes investigated.					
34813787	10	18	located	detected	1632:1639	arg1	processes					1654:1662	enzymatic processes	1644:1662	enzymatic processes investigated	1644:1675	Regardless of the structural diversity of the ulvan samples there was very little antioxidant or inhibitory activity detected on enzymatic processes investigated.					
34813787	10	18	located	detected	1632:1639	arg2	little					1590:1595	little	1590:1595	little	1590:1595	Regardless of the structural diversity of the ulvan samples there was very little antioxidant or inhibitory activity detected on enzymatic processes investigated.					
34813787	2	19	theme	3.4-10.4 mol	430:441	arg1	%					442:442	3.4-10.4 mol%	430:442	3.4-10.4 mol%	430:442	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	19	theme	3.4-10.4 mol	430:441	arg1	xylose					422:427	xylose	422:427	xylose (3.4-10.4 mol%)	422:443	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	8	20	theme	ulvanobiuronic	1243:1256	arg1	repeats					1263:1269	type B3S ulvanobiuronic acid repeats	1234:1269	type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1234:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	20	theme	ulvanobiuronic	1243:1256	arg1	ulvanobiose					1312:1322	ulvanobiose	1312:1322	ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1312:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	20	theme	ulvanobiuronic	1243:1256	arg1	-α-L-Rhap3S-					1291:1302	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	4	21	dep	-Rhap-	650:655	arg1	→3,4					645:648	1→ and →3,4	638:648	1→ and →3,4	638:648	Glycosyl linkage analysis showed that ulvan from U. ohnoi contained mostly →4)-GlcpA-(1→ and →3,4)-Rhap-(1→.					
34813787	6	22	theme	prolifera	855:863	arg1	analysis					826:833	glycosyl linkage analysis	809:833	glycosyl linkage analysis of U. tepida and U. prolifera	809:863	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	8	23	theme	repeats	1263:1269	arg1	repeats					1263:1269	type B3S ulvanobiuronic acid repeats	1234:1269	type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1234:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	23	theme	repeats	1263:1269	arg1	ulvanobiose					1312:1322	ulvanobiose	1312:1322	ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1312:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	23	theme	repeats	1263:1269	arg1	-α-L-Rhap3S-					1291:1302	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	23	theme	repeats	1263:1269	arg1	amounts					1223:1229	smaller amounts	1215:1229	smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1215:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	24	theme	type	1126:1129	arg1	repeats					1155:1161	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	0	25	theme	filamentous	76:86	arg1	species					121:127	blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species	53:127	species	121:127	Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva.					
34813787	2	26	contain	contained	317:325	arg2	%					394:394	26.6-37.5 mol%	381:394	26.6-37.5 mol%	381:394	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	26	contain	contained	317:325	arg2	acid					375:378	glucuronic acid	364:378	glucuronic acid (26.6-37.5 mol%)	364:395	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	26	contain	contained	317:325	arg1	ulvans					310:315	The ulvans	306:315	The ulvans	306:315	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	26	contain	contained	317:325	arg2	%					357:357	31.6-46.7 mol%	344:357	31.6-46.7 mol%	344:357	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	26	contain	contained	317:325	arg2	rhamnose					334:341	rhamnose	334:341	rhamnose (31.6-46.7 mol%)	334:358	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	9	27	located	detected	1431:1438	arg1	ohnoi					1446:1450	ohnoi	1446:1450	ohnoi	1446:1450	NMR spectra of U. tepida and U. prolifera showed resonances not detected in U. ohnoi, highlighting the complexity of the ulvans from these species.					
34813787	9	27	located	detected	1431:1438	arg2	resonances					1416:1425	resonances	1416:1425	resonances not detected in U. ohnoi	1416:1450	NMR spectra of U. tepida and U. prolifera showed resonances not detected in U. ohnoi, highlighting the complexity of the ulvans from these species.					
34813787	2	28	theme	iduronic	449:456	arg1	acid					458:461	iduronic acid	449:461	iduronic acid (3.1-7.6 mol%)	449:476	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	28	theme	iduronic	449:456	arg1	%					475:475	3.1-7.6 mol%	464:475	3.1-7.6 mol%	464:475	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	8	29	theme	ulvanobiuronic	1135:1148	arg1	repeats					1155:1161	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	4	30	theme	1→ and	638:643	arg1	→3,4					645:648	1→ and →3,4	638:648	1→ and →3,4	638:648	Glycosyl linkage analysis showed that ulvan from U. ohnoi contained mostly →4)-GlcpA-(1→ and →3,4)-Rhap-(1→.					
34813787	5	31	theme	acetate	705:711	arg1	standards					713:721	partially methylated alditol acetate standards	676:721	partially methylated alditol acetate standards of idose	676:730	Preparation of partially methylated alditol acetate standards of idose showed that U. ohnoi contained →4)-IdopA-(1→.					
34813787	9	32	theme	ulvans	1488:1493	arg1	complexity					1470:1479	the complexity	1466:1479	the complexity of the ulvans from these species	1466:1512	NMR spectra of U. tepida and U. prolifera showed resonances not detected in U. ohnoi, highlighting the complexity of the ulvans from these species.					
34813787	2	33	theme	26.6-37.5 mol	381:393	arg1	acid					375:378	glucuronic acid	364:378	glucuronic acid (26.6-37.5 mol%)	364:395	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	33	theme	26.6-37.5 mol	381:393	arg1	%					394:394	26.6-37.5 mol%	381:394	26.6-37.5 mol%	381:394	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	34	theme	xylose	422:427	arg1	%					442:442	3.4-10.4 mol%	430:442	3.4-10.4 mol%	430:442	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	34	theme	xylose	422:427	arg1	amounts					411:417	smaller amounts	403:417	smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%)	403:476	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	34	theme	xylose	422:427	arg1	acid					458:461	iduronic acid	449:461	iduronic acid (3.1-7.6 mol%)	449:476	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	34	theme	xylose	422:427	arg1	%					475:475	3.1-7.6 mol%	464:475	3.1-7.6 mol%	464:475	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	34	theme	xylose	422:427	arg1	xylose					422:427	xylose	422:427	xylose (3.4-10.4 mol%)	422:443	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	10	35	theme	structural	1533:1542	arg1	diversity					1544:1552	the structural diversity	1529:1552	the structural diversity of the ulvan samples	1529:1573	Regardless of the structural diversity of the ulvan samples there was very little antioxidant or inhibitory activity detected on enzymatic processes investigated.					
34813787	5	36	theme	methylated	686:695	arg1	standards					713:721	partially methylated alditol acetate standards	676:721	partially methylated alditol acetate standards of idose	676:730	Preparation of partially methylated alditol acetate standards of idose showed that U. ohnoi contained →4)-IdopA-(1→.					
34813787	10	37	theme	ulvan	1561:1565	arg1	samples					1567:1573	the ulvan samples	1557:1573	the ulvan samples	1557:1573	Regardless of the structural diversity of the ulvan samples there was very little antioxidant or inhibitory activity detected on enzymatic processes investigated.					
34813787	7	38	contain	contained	980:988	arg1	species					967:973	These two species	957:973	These two species	957:973	These two species also contained galactose linkages.					
34813787	7	38	contain	contained	980:988	arg2	linkages					1000:1007	galactose linkages	990:1007	galactose linkages	990:1007	These two species also contained galactose linkages.					
34813787	8	39	dep	indicated	1082:1090	arg1	ohnoi					1100:1104	ohnoi	1100:1104	ohnoi	1100:1104	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	40	theme	U3S	1325:1327	arg1	ulvanobiose					1312:1322	ulvanobiose	1312:1322	ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1312:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	40	theme	U3S	1325:1327	arg1	-α-L-Rhap3S-					1348:1359	U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]	1325:1363	U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]	1325:1363	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	4	41	from	ohnoi	604:608	arg1	ulvan					590:594	ulvan	590:594	ulvan from U. ohnoi	590:608	Glycosyl linkage analysis showed that ulvan from U. ohnoi contained mostly →4)-GlcpA-(1→ and →3,4)-Rhap-(1→.					
34813787	1	42	theme	mild	225:228	arg1	conditions					237:246	mild acidic conditions	225:246	mild acidic conditions	225:246	Ulvans from Ulva ohnoi, Ulva tepida and Ulva prolifera were extracted under mild acidic conditions, isolated and their composition and structure determined.					
34813787	10	43	theme	inhibitory	1612:1621	arg1	activity					1623:1630	inhibitory activity	1612:1630	inhibitory activity	1612:1630	Regardless of the structural diversity of the ulvan samples there was very little antioxidant or inhibitory activity detected on enzymatic processes investigated.					
34813787	9	44	theme	NMR	1367:1369	arg1	spectra					1371:1377	NMR spectra	1367:1377	NMR spectra of U. tepida and U. prolifera	1367:1407	NMR spectra of U. tepida and U. prolifera showed resonances not detected in U. ohnoi, highlighting the complexity of the ulvans from these species.					
34813787	6	45	theme	1→	953:954	arg1	presence					876:883	the presence	872:883	the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	872:954	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	8	46	dep	repeats	1155:1161	arg1	1→					1196:1197	1→	1196:1197	1→	1196:1197	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	46	dep	repeats	1155:1161	arg1	-α-L-Rhap3S-					1183:1194	[→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-	1163:1194	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	6	47	theme	tepida	841:846	arg1	analysis					826:833	glycosyl linkage analysis	809:833	glycosyl linkage analysis of U. tepida and U. prolifera	809:863	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	6	48	theme	-GlcpA-	945:951	arg1	1→					953:954	→2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	888:954	1→	953:954	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	10	49	theme	enzymatic	1644:1652	arg1	processes					1654:1662	enzymatic processes	1644:1662	enzymatic processes investigated	1644:1675	Regardless of the structural diversity of the ulvan samples there was very little antioxidant or inhibitory activity detected on enzymatic processes investigated.					
34813787	5	50	dep	contained	753:761	arg1	1→					774:775	1→	774:775	1→	774:775	Preparation of partially methylated alditol acetate standards of idose showed that U. ohnoi contained →4)-IdopA-(1→.					
34813787	5	51	theme	idose	726:730	arg1	standards					713:721	partially methylated alditol acetate standards	676:721	partially methylated alditol acetate standards of idose	676:730	Preparation of partially methylated alditol acetate standards of idose showed that U. ohnoi contained →4)-IdopA-(1→.					
34813787	9	52	theme	tepida	1385:1390	arg1	spectra					1371:1377	NMR spectra	1367:1377	NMR spectra of U. tepida and U. prolifera	1367:1407	NMR spectra of U. tepida and U. prolifera showed resonances not detected in U. ohnoi, highlighting the complexity of the ulvans from these species.					
34813787	6	53	theme	→3,4	940:943	arg1	1→					953:954	→2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	888:954	1→	953:954	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	8	54	theme	magnetic	1044:1051	arg1	resonance					1053:1061	nuclear magnetic resonance	1036:1061	nuclear magnetic resonance (NMR) spectroscopy	1036:1080	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	54	theme	magnetic	1044:1051	arg1	NMR					1064:1066	NMR	1064:1066	NMR	1064:1066	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	6	55	theme	linkage	818:824	arg1	analysis					826:833	glycosyl linkage analysis	809:833	glycosyl linkage analysis of U. tepida and U. prolifera	809:863	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	2	56	theme	3.1-7.6 mol	464:474	arg1	acid					458:461	iduronic acid	449:461	iduronic acid (3.1-7.6 mol%)	449:476	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	56	theme	3.1-7.6 mol	464:474	arg1	%					475:475	3.1-7.6 mol%	464:475	3.1-7.6 mol%	464:475	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	8	57	theme	smaller	1215:1221	arg1	repeats					1263:1269	type B3S ulvanobiuronic acid repeats	1234:1269	type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1234:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	57	theme	smaller	1215:1221	arg1	ulvanobiose					1312:1322	ulvanobiose	1312:1322	ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1312:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	57	theme	smaller	1215:1221	arg1	-α-L-Rhap3S-					1291:1302	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	57	theme	smaller	1215:1221	arg1	amounts					1223:1229	smaller amounts	1215:1229	smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1215:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	4	58	theme	Glycosyl	552:559	arg1	analysis					569:576	Glycosyl linkage analysis	552:576	Glycosyl linkage analysis	552:576	Glycosyl linkage analysis showed that ulvan from U. ohnoi contained mostly →4)-GlcpA-(1→ and →3,4)-Rhap-(1→.					
34813787	1	59	from	prolifera	194:202	arg1	Ulvans					149:154	Ulvans	149:154	Ulvans from Ulva ohnoi, Ulva tepida and Ulva prolifera	149:202	Ulvans from Ulva ohnoi, Ulva tepida and Ulva prolifera were extracted under mild acidic conditions, isolated and their composition and structure determined.					
34813787	3	60	theme	ulvan	496:500	arg1	samples					502:508	the ulvan samples	492:508	the ulvan samples	492:508	In addition, the ulvan samples also contained galactose (4.4-26.0 mol%).					
34813787	1	61	from	tepida	178:183	arg1	Ulvans					149:154	Ulvans	149:154	Ulvans from Ulva ohnoi, Ulva tepida and Ulva prolifera	149:202	Ulvans from Ulva ohnoi, Ulva tepida and Ulva prolifera were extracted under mild acidic conditions, isolated and their composition and structure determined.					
34813787	0	62	theme	Ulva	143:146	arg1	blade					53:57	blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species	53:127	blade	53:57	Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva.					
34813787	0	62	theme	Ulva	143:146	arg1	ohnoi					65:69	ohnoi	65:69	ohnoi	65:69	Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva.					
34813787	0	62	theme	Ulva	143:146	arg1	species					121:127	blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species	53:127	species	121:127	Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva.					
34813787	3	63	theme	4.4-26.0 mol	536:547	arg1	galactose					525:533	galactose	525:533	galactose (4.4-26.0 mol%)	525:549	In addition, the ulvan samples also contained galactose (4.4-26.0 mol%).					
34813787	3	63	theme	4.4-26.0 mol	536:547	arg1	%					548:548	4.4-26.0 mol%	536:548	4.4-26.0 mol%	536:548	In addition, the ulvan samples also contained galactose (4.4-26.0 mol%).					
34813787	8	64	theme	B3S	1239:1241	arg1	repeats					1263:1269	type B3S ulvanobiuronic acid repeats	1234:1269	type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1234:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	64	theme	B3S	1239:1241	arg1	ulvanobiose					1312:1322	ulvanobiose	1312:1322	ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1312:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	64	theme	B3S	1239:1241	arg1	-α-L-Rhap3S-					1291:1302	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	65	theme	acid	1258:1261	arg1	repeats					1263:1269	type B3S ulvanobiuronic acid repeats	1234:1269	type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1234:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	65	theme	acid	1258:1261	arg1	ulvanobiose					1312:1322	ulvanobiose	1312:1322	ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1312:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	65	theme	acid	1258:1261	arg1	-α-L-Rhap3S-					1291:1302	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	7	66	theme	galactose	990:998	arg1	linkages					1000:1007	galactose linkages	990:1007	galactose linkages	990:1007	These two species also contained galactose linkages.					
34813787	4	67	dep	contained	610:618	arg1	1→					657:658	1→	657:658	1→	657:658	Glycosyl linkage analysis showed that ulvan from U. ohnoi contained mostly →4)-GlcpA-(1→ and →3,4)-Rhap-(1→.					
34813787	2	68	theme	acid	458:461	arg1	%					442:442	3.4-10.4 mol%	430:442	3.4-10.4 mol%	430:442	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	68	theme	acid	458:461	arg1	amounts					411:417	smaller amounts	403:417	smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%)	403:476	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	68	theme	acid	458:461	arg1	acid					458:461	iduronic acid	449:461	iduronic acid (3.1-7.6 mol%)	449:476	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	68	theme	acid	458:461	arg1	%					475:475	3.1-7.6 mol%	464:475	3.1-7.6 mol%	464:475	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	68	theme	acid	458:461	arg1	xylose					422:427	xylose	422:427	xylose (3.4-10.4 mol%)	422:443	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	8	69	theme	-α-L-IdopA-	1275:1285	arg1	repeats					1263:1269	type B3S ulvanobiuronic acid repeats	1234:1269	type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1234:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	69	theme	-α-L-IdopA-	1275:1285	arg1	-α-L-Rhap3S-					1291:1302	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	[→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→]	1271:1306	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	5	70	contain	contained	753:761	arg2	-IdopA-					766:772	→4)-IdopA-	763:772	→4)-IdopA-	763:772	Preparation of partially methylated alditol acetate standards of idose showed that U. ohnoi contained →4)-IdopA-(1→.					
34813787	5	70	contain	contained	753:761	arg1	ohnoi					747:751	U. ohnoi	744:751	U. ohnoi	744:751	Preparation of partially methylated alditol acetate standards of idose showed that U. ohnoi contained →4)-IdopA-(1→.					
34813787	6	71	dep	1→	953:954	arg1	→4					906:907	→2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	888:954	→4	906:907	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	6	71	dep	1→	953:954	arg1	→2,4					920:923	→2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	888:954	→2,4	920:923	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	6	72	theme	-Xylp-	909:914	arg1	1→					953:954	→2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	888:954	1→	953:954	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	8	73	theme	A3S	1131:1133	arg1	repeats					1155:1161	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	5	74	theme	alditol	697:703	arg1	standards					713:721	partially methylated alditol acetate standards	676:721	partially methylated alditol acetate standards of idose	676:730	Preparation of partially methylated alditol acetate standards of idose showed that U. ohnoi contained →4)-IdopA-(1→.					
34813787	8	75	theme	acid	1150:1153	arg1	repeats					1155:1161	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	2	76	theme	31.6-46.7 mol	344:356	arg1	rhamnose					334:341	rhamnose	334:341	rhamnose (31.6-46.7 mol%)	334:358	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	76	theme	31.6-46.7 mol	344:356	arg1	%					357:357	31.6-46.7 mol%	344:357	31.6-46.7 mol%	344:357	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	0	77	theme	ulvans	31:36	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of ulvans	0:36	Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva.					
34813787	0	78	dep	ohnoi	65:69	arg1	Ulva					60:63	Ulva	60:63	Ulva	60:63	Structural characterization of ulvans extracted from blade (Ulva ohnoi) and filamentous (Ulva tepida and Ulva prolifera) species of cultivated Ulva.					
34813787	6	79	theme	1→	902:903	arg1	1→					953:954	→2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	888:954	1→	953:954	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	9	80	from	complexity	1470:1479	arg1	species					1506:1512	these species	1500:1512	these species	1500:1512	NMR spectra of U. tepida and U. prolifera showed resonances not detected in U. ohnoi, highlighting the complexity of the ulvans from these species.					
34813787	6	81	theme	-Rhap-	895:900	arg1	1→					953:954	→2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	888:954	1→	953:954	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	8	82	theme	-β-D-GlcpA-	1167:1177	arg1	1→					1196:1197	1→	1196:1197	1→	1196:1197	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	82	theme	-β-D-GlcpA-	1167:1177	arg1	-α-L-Rhap3S-					1183:1194	[→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-	1163:1194	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	2	83	theme	glucuronic	364:373	arg1	acid					375:378	glucuronic acid	364:378	glucuronic acid (26.6-37.5 mol%)	364:395	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	83	theme	glucuronic	364:373	arg1	%					394:394	26.6-37.5 mol%	381:394	26.6-37.5 mol%	381:394	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	6	84	theme	1→ and	933:938	arg1	1→					953:954	→2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	888:954	1→	953:954	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	2	85	theme	smaller	403:409	arg1	%					442:442	3.4-10.4 mol%	430:442	3.4-10.4 mol%	430:442	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	85	theme	smaller	403:409	arg1	amounts					411:417	smaller amounts	403:417	smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%)	403:476	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	85	theme	smaller	403:409	arg1	acid					458:461	iduronic acid	449:461	iduronic acid (3.1-7.6 mol%)	449:476	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	85	theme	smaller	403:409	arg1	%					475:475	3.1-7.6 mol%	464:475	3.1-7.6 mol%	464:475	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	2	85	theme	smaller	403:409	arg1	xylose					422:427	xylose	422:427	xylose (3.4-10.4 mol%)	422:443	The ulvans contained mostly rhamnose (31.6-46.7 mol%) and glucuronic acid (26.6-37.5 mol%), with smaller amounts of xylose (3.4-10.4 mol%) and iduronic acid (3.1-7.6 mol%).					
34813787	10	86	dep	little	1590:1595	arg1	antioxidant					1597:1607	antioxidant	1597:1607	antioxidant	1597:1607	Regardless of the structural diversity of the ulvan samples there was very little antioxidant or inhibitory activity detected on enzymatic processes investigated.					
34813787	10	87	theme	samples	1567:1573	arg1	diversity					1544:1552	the structural diversity	1529:1552	the structural diversity of the ulvan samples	1529:1573	Regardless of the structural diversity of the ulvan samples there was very little antioxidant or inhibitory activity detected on enzymatic processes investigated.					
34813787	8	88	theme	1→4	1179:1181	arg1	1→					1196:1197	1→	1196:1197	1→	1196:1197	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	88	theme	1→4	1179:1181	arg1	-α-L-Rhap3S-					1183:1194	[→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-	1163:1194	type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→]	1126:1198	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	6	89	theme	-GlcpA-	925:931	arg1	1→					953:954	→2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→	888:954	1→	953:954	In addition to these residues, glycosyl linkage analysis of U. tepida and U. prolifera showed the presence of →2,3,4)-Rhap-(1→, →4)-Xylp-(1→, →2,4)-GlcpA-(1→ and →3,4)-GlcpA-(1→.					
34813787	8	90	theme	-β-D-Xylp-	1333:1342	arg1	ulvanobiose					1312:1322	ulvanobiose	1312:1322	ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→])	1312:1364	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
34813787	8	90	theme	-β-D-Xylp-	1333:1342	arg1	-α-L-Rhap3S-					1348:1359	U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]	1325:1363	U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]	1325:1363	These data, together with nuclear magnetic resonance (NMR) spectroscopy indicated that U. ohnoi comprised mostly of type A3S ulvanobiuronic acid repeats [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3S-(1→], together with smaller amounts of type B3S ulvanobiuronic acid repeats [→4)-α-L-IdopA-(1→4)-α-L-Rhap3S-(1→] and ulvanobiose (U3S [→4)-β-D-Xylp-(1→4)-α-L-Rhap3S-(1→]).					
33459320	1	0	contain	possessing	142:151	arg1	oligosaccharides					107:122	complex oligosaccharides	99:122	complex oligosaccharides	99:122	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	1	0	contain	possessing	142:151	arg1	those					136:140	those	136:140	those	136:140	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	1	0	contain	possessing	142:151	arg2	structures					168:177	hyper-branched structures	153:177	hyper-branched structures	153:177	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	3	1	theme	hyper-branched	565:578	arg1	motif					601:605	a semi-conserved hyper-branched core tetrasaccharide motif	548:605	a semi-conserved hyper-branched core tetrasaccharide motif	548:605	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	3	2	from	chloroviruses	612:624	arg1	synthesis					535:543	the synthesis	531:543	the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models	531:714	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	2	3	theme	several	462:468	arg1	factors					470:476	several factors	462:476	several factors that make such syntheses daunting	462:510	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	3	4	theme	core	580:583	arg1	motif					601:605	a semi-conserved hyper-branched core tetrasaccharide motif	548:605	a semi-conserved hyper-branched core tetrasaccharide motif	548:605	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	5	5	theme	comprehensive	974:986	arg1	study					988:992	A thorough and comprehensive study	959:992	A thorough and comprehensive study of various accountable factors	959:1023	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	3	6	theme	mouse	703:707	arg1	models					709:714	mouse models	703:714	mouse models	703:714	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	4	7	theme	1,2-trans	847:855	arg1	bond					868:871	a 1,2-trans glycosidic bond	845:871	a 1,2-trans glycosidic bond	845:871	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	5	8	theme	stereoselective	1089:1103	arg1	fashion					1105:1111	a stereoselective fashion	1087:1111	a stereoselective fashion	1087:1111	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	5	9	theme	1,2-cis	1049:1055	arg1	linkage					1076:1082	a 1,2-cis galactopyranosidic linkage	1047:1082	a 1,2-cis galactopyranosidic linkage	1047:1082	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	3	10	theme	reduced	652:658	arg1	function					670:677	reduced cognitive function	652:677	reduced cognitive function in humans as well as in mouse models	652:714	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	5	11	theme	tetrasaccharide	1187:1201	arg1	motif					1203:1207	the target tetrasaccharide motif	1176:1207	the target tetrasaccharide motif in 14 steps	1176:1219	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	5	12	theme	galactopyranosidic	1057:1074	arg1	linkage					1076:1082	a 1,2-cis galactopyranosidic linkage	1047:1082	a 1,2-cis galactopyranosidic linkage	1047:1082	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	2	13	theme	proper	311:316	arg1	tuning					318:323	proper tuning	311:323	proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors	311:450	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	1	14	theme	Chemical	77:84	arg1	task					243:246	a challenging task	229:246	a challenging task	229:246	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	1	14	theme	Chemical	77:84	arg1	synthesis					86:94	Chemical synthesis	77:94	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds,	77:224	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	4	15	theme	different	758:766	arg1	residues					774:781	four different sugar residues	753:781	four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond	753:871	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	1	16	theme	multiple	191:198	arg1	1,2-cis					200:206	multiple 1,2-cis	191:206	multiple 1,2-cis	191:206	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	2	17	theme	acceptors	297:305	arg1	tuning					318:323	proper tuning	311:323	proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors	311:450	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	2	17	theme	acceptors	297:305	arg1	reactivity					263:272	Complementary reactivity	249:272	Complementary reactivity of glycosyl donors and acceptors	249:305	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	3	18	theme	motif	601:605	arg1	synthesis					535:543	the synthesis	531:543	the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models	531:714	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	4	19	theme	glycosidic	942:951	arg1	bond					953:956	a 1,2-cis glycosidic bond	932:956	a 1,2-cis glycosidic bond	932:956	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	1	20	dep	one	184:186	arg1	bonds					219:223	glycosidic bonds	208:223	glycosidic bonds	208:223	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	5	21	theme	accountable	1005:1015	arg1	factors					1017:1023	various accountable factors	997:1023	various accountable factors	997:1023	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	1	22	theme	glycosidic	208:217	arg1	bonds					219:223	glycosidic bonds	208:223	glycosidic bonds	208:223	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	4	23	theme	1,2-cis	934:940	arg1	bond					953:956	a 1,2-cis glycosidic bond	932:956	a 1,2-cis glycosidic bond	932:956	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	2	24	theme	solvent/temperature/activator	332:360	arg1	tuning					318:323	proper tuning	311:323	proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors	311:450	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	2	24	theme	solvent/temperature/activator	332:360	arg1	reactivity					263:272	Complementary reactivity	249:272	Complementary reactivity of glycosyl donors and acceptors	249:305	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	3	25	from	function	670:677	arg1	humans					682:687	humans	682:687	humans	682:687	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	3	25	from	function	670:677	arg1	models					709:714	mouse models	703:714	mouse models	703:714	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	5	26	theme	thorough	961:968	arg1	study					988:992	A thorough and comprehensive study	959:992	A thorough and comprehensive study of various accountable factors	959:1023	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	0	27	theme	hyper-branched	17:30	arg1	motif					53:57	the hyper-branched core tetrasaccharide motif	13:57	the hyper-branched core tetrasaccharide motif of chloroviruses	13:74	Synthesis of the hyper-branched core tetrasaccharide motif of chloroviruses.					
33459320	4	28	dep	contains	744:751	arg1	with					882:885	with	882:885	with	882:885	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	4	28	dep	contains	744:751	arg1	whereas					874:880	whereas	874:880	whereas	874:880	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	1	29	theme	oligosaccharides	107:122	arg1	task					243:246	a challenging task	229:246	a challenging task	229:246	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	1	29	theme	oligosaccharides	107:122	arg1	synthesis					86:94	Chemical synthesis	77:94	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds,	77:224	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	2	30	theme	such	488:491	arg1	syntheses					493:501	such syntheses	488:501	such syntheses	488:501	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	1	31	theme	complex	99:105	arg1	oligosaccharides					107:122	complex oligosaccharides	99:122	complex oligosaccharides	99:122	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	1	31	theme	complex	99:105	arg1	those					136:140	those	136:140	those	136:140	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	3	32	theme	tetrasaccharide	585:599	arg1	motif					601:605	a semi-conserved hyper-branched core tetrasaccharide motif	548:605	a semi-conserved hyper-branched core tetrasaccharide motif	548:605	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	0	33	theme	tetrasaccharide	37:51	arg1	motif					53:57	the hyper-branched core tetrasaccharide motif	13:57	the hyper-branched core tetrasaccharide motif of chloroviruses	13:74	Synthesis of the hyper-branched core tetrasaccharide motif of chloroviruses.					
33459320	3	34	theme	semi-conserved	550:563	arg1	motif					601:605	a semi-conserved hyper-branched core tetrasaccharide motif	548:605	a semi-conserved hyper-branched core tetrasaccharide motif	548:605	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	4	35	theme	target	721:726	arg1	tetrasaccharide					728:742	The target tetrasaccharide	717:742	The target tetrasaccharide	717:742	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	3	36	theme	cognitive	660:668	arg1	function					670:677	reduced cognitive function	652:677	reduced cognitive function in humans as well as in mouse models	652:714	Herein, we report the synthesis of a semi-conserved hyper-branched core tetrasaccharide motif from chloroviruses which are associated with reduced cognitive function in humans as well as in mouse models.					
33459320	0	37	theme	core	32:35	arg1	motif					53:57	the hyper-branched core tetrasaccharide motif	13:57	the hyper-branched core tetrasaccharide motif of chloroviruses	13:74	Synthesis of the hyper-branched core tetrasaccharide motif of chloroviruses.					
33459320	2	38	theme	Complementary	249:261	arg1	reactivity					263:272	Complementary reactivity	249:272	Complementary reactivity of glycosyl donors and acceptors	249:305	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	1	39	theme	challenging	231:241	arg1	task					243:246	a challenging task	229:246	a challenging task	229:246	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	1	39	theme	challenging	231:241	arg1	synthesis					86:94	Chemical synthesis	77:94	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds,	77:224	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	5	40	theme	target	1180:1185	arg1	motif					1203:1207	the target tetrasaccharide motif	1176:1207	the target tetrasaccharide motif in 14 steps	1176:1219	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	5	41	theme	various	997:1003	arg1	factors					1017:1023	various accountable factors	997:1023	various accountable factors	997:1023	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	0	42	theme	motif	53:57	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of the hyper-branched core tetrasaccharide motif of chloroviruses	0:74	Synthesis of the hyper-branched core tetrasaccharide motif of chloroviruses.					
33459320	5	43	theme	-catalyzed	1128:1137	arg1	conditions					1153:1162	[Au]/[Ag]-catalyzed glycosidation conditions	1119:1162	[Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps	1119:1219	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	2	44	theme	glycosylation	387:399	arg1	yields					401:406	compromised glycosylation yields	375:406	compromised glycosylation yields for sterically congested glycosyl acceptors	375:450	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	4	45	theme	d-galactose	891:901	arg1	residue					903:909	the d-galactose residue	887:909	the d-galactose residue	887:909	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	5	46	theme	glycosidation	1139:1151	arg1	conditions					1153:1162	[Au]/[Ag]-catalyzed glycosidation conditions	1119:1162	[Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps	1119:1219	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	4	47	theme	sugar	768:772	arg1	residues					774:781	four different sugar residues	753:781	four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond	753:871	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	2	48	theme	compromised	375:385	arg1	yields					401:406	compromised glycosylation yields	375:406	compromised glycosylation yields for sterically congested glycosyl acceptors	375:450	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	4	49	theme	glycosidic	857:866	arg1	bond					868:871	a 1,2-trans glycosidic bond	845:871	a 1,2-trans glycosidic bond	845:871	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	2	50	theme	donors	286:291	arg1	tuning					318:323	proper tuning	311:323	proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors	311:450	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	2	50	theme	donors	286:291	arg1	reactivity					263:272	Complementary reactivity	249:272	Complementary reactivity of glycosyl donors and acceptors	249:305	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	1	51	theme	hyper-branched	153:166	arg1	structures					168:177	hyper-branched structures	153:177	hyper-branched structures	153:177	Chemical synthesis of complex oligosaccharides, especially those possessing hyper-branched structures with one or multiple 1,2-cis glycosidic bonds, is a challenging task.					
33459320	5	52	theme	factors	1017:1023	arg1	study					988:992	A thorough and comprehensive study	959:992	A thorough and comprehensive study of various accountable factors	959:1023	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	4	53	contain	contains	744:751	arg2	residues					774:781	four different sugar residues	753:781	four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond	753:871	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	4	53	contain	contains	744:751	arg1	tetrasaccharide					728:742	The target tetrasaccharide	717:742	The target tetrasaccharide	717:742	The target tetrasaccharide contains four different sugar residues in which l-fucose is connected to d-xylose and l-rhamnose via a 1,2-trans glycosidic bond, whereas with the d-galactose residue is connected through a 1,2-cis glycosidic bond.					
33459320	2	54	theme	glycosyl	277:284	arg1	donors					286:291	glycosyl donors	277:291	glycosyl donors	277:291	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	2	55	theme	glycosyl	433:440	arg1	acceptors					442:450	sterically congested glycosyl acceptors	412:450	sterically congested glycosyl acceptors	412:450	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
33459320	0	56	theme	chloroviruses	62:74	arg1	motif					53:57	the hyper-branched core tetrasaccharide motif	13:57	the hyper-branched core tetrasaccharide motif of chloroviruses	13:74	Synthesis of the hyper-branched core tetrasaccharide motif of chloroviruses.					
33459320	5	57	from	motif	1203:1207	arg1	steps					1215:1219	14 steps	1212:1219	14 steps	1212:1219	A thorough and comprehensive study of various accountable factors enabled us to install a 1,2-cis galactopyranosidic linkage in a stereoselective fashion under [Au]/[Ag]-catalyzed glycosidation conditions en route to the target tetrasaccharide motif in 14 steps.					
33459320	2	58	theme	congested	423:431	arg1	acceptors					442:450	sterically congested glycosyl acceptors	412:450	sterically congested glycosyl acceptors	412:450	Complementary reactivity of glycosyl donors and acceptors and proper tuning of the solvent/temperature/activator coupled with compromised glycosylation yields for sterically congested glycosyl acceptors are among several factors that make such syntheses daunting.					
34735142	0	0	theme	Microalgal	77:86	arg1	Polysaccharides					88:102	Microalgal Polysaccharides	77:102	Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance	77:154	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	0	1	from	Quantification	19:32	arg1	Cells					54:58	Whole Cells	48:58	Whole Cells	48:58	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	4	2	theme	13C-13C	771:777	arg1	spectra					797:803	Two-dimensional 13C-13C correlation ssNMR spectra	755:803	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii	755:873	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	5	3	theme	flat-ribbon	1188:1198	arg1	xylan					1207:1211	the flat-ribbon 2-fold xylan	1184:1211	the flat-ribbon 2-fold xylan identified in plants	1184:1232	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	1	4	theme	photosynthetic	172:185	arg1	Microalgae					157:166	Microalgae	157:166	Microalgae	157:166	Microalgae are photosynthetic organisms widely distributed in nature and serve as a sustainable source of bioproducts.					
34735142	1	4	theme	photosynthetic	172:185	arg1	organisms					187:195	photosynthetic organisms	172:195	photosynthetic organisms widely distributed in nature	172:224	Microalgae are photosynthetic organisms widely distributed in nature and serve as a sustainable source of bioproducts.					
34735142	5	5	dep	domains	1108:1114	arg1	both					1082:1085	both	1082:1085	both	1082:1085	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	2	6	theme	structural	389:398	arg1	characterization					400:415	the structural characterization	385:415	the structural characterization of these heterogeneous polymers in cells	385:456	Their carbohydrate components are also promising candidates for bioenergy production and bioremediation, but the structural characterization of these heterogeneous polymers in cells remains a formidable problem.					
34735142	0	7	from	Identification	0:13	arg1	Cells					54:58	Whole Cells	48:58	Whole Cells	48:58	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	6	8	theme	most	1249:1252	arg1	carbohydrates					1260:1272	most other carbohydrates	1249:1272	most other carbohydrates	1249:1272	Surprisingly, most other carbohydrates are largely mobile, regardless of their distribution in glycolipids or cell walls.					
34735142	3	9	theme	MAS	612:614	arg1	spectroscopy					641:652	magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	590:652	magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	590:652	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	3	10	theme	NMR	629:631	arg1	spectroscopy					641:652	magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	590:652	magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	590:652	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	0	11	theme	Polysaccharides	88:102	arg1	Architecture					61:72	Architecture	61:72	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.	0:155	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	4	12	theme	microalga	838:846	arg1	beijerinckii					862:873	a uniformly 13C-labeled green microalga Parachlorella beijerinckii	808:873	a uniformly 13C-labeled green microalga Parachlorella beijerinckii	808:873	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	2	13	theme	promising	315:323	arg1	candidates					325:334	promising candidates	315:334	promising candidates for bioenergy production and bioremediation	315:378	Their carbohydrate components are also promising candidates for bioenergy production and bioremediation, but the structural characterization of these heterogeneous polymers in cells remains a formidable problem.					
34735142	2	13	theme	promising	315:323	arg1	components					295:304	Their carbohydrate components	276:304	Their carbohydrate components	276:304	Their carbohydrate components are also promising candidates for bioenergy production and bioremediation, but the structural characterization of these heterogeneous polymers in cells remains a formidable problem.					
34735142	0	14	theme	Solid-State	117:127	arg1	Resonance					146:154	Solid-State Nuclear Magnetic Resonance	117:154	Solid-State Nuclear Magnetic Resonance	117:154	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	7	15	theme	structural	1363:1372	arg1	insights					1374:1381	These structural insights	1357:1381	These structural insights	1357:1381	These structural insights correlate with the high digestibility of this cellulose-deficient strain, and the in-cell ssNMR methods will facilitate the investigations of other economically important algae species.					
34735142	7	16	theme	high	1402:1405	arg1	digestibility					1407:1419	the high digestibility	1398:1419	the high digestibility of this cellulose-deficient strain	1398:1454	These structural insights correlate with the high digestibility of this cellulose-deficient strain, and the in-cell ssNMR methods will facilitate the investigations of other economically important algae species.					
34735142	3	17	theme	magic-angle-spinning	590:609	arg1	spectroscopy					641:652	magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	590:652	magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	590:652	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	7	18	theme	species	1560:1566	arg1	investigations					1507:1520	the investigations	1503:1520	the investigations of other economically important algae species	1503:1566	These structural insights correlate with the high digestibility of this cellulose-deficient strain, and the in-cell ssNMR methods will facilitate the investigations of other economically important algae species.					
34735142	6	19	theme	cell	1345:1348	arg1	walls					1350:1354	cell walls	1345:1354	cell walls	1345:1354	Surprisingly, most other carbohydrates are largely mobile, regardless of their distribution in glycolipids or cell walls.					
34735142	2	20	from	characterization	400:415	arg1	cells					452:456	cells	452:456	cells	452:456	Their carbohydrate components are also promising candidates for bioenergy production and bioremediation, but the structural characterization of these heterogeneous polymers in cells remains a formidable problem.					
34735142	4	21	theme	beijerinckii	862:873	arg1	spectra					797:803	Two-dimensional 13C-13C correlation ssNMR spectra	755:803	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii	755:873	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	3	22	from	analysis	709:716	arg1	validation					660:669	validation	660:669	validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS)	660:752	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	5	23	located	present	1071:1077	arg1	domains					1108:1114	both the mobile and rigid domains	1082:1114	domains	1108:1114	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	5	23	located	present	1071:1077	arg2	xyloses					1059:1065	Some xyloses	1054:1065	Some xyloses	1054:1065	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	5	23	located	present	1071:1077	arg1	wall					1128:1131	the cell wall	1119:1131	the cell wall	1119:1131	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	3	24	from	linkage	685:691	arg1	validation					660:669	validation	660:669	validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS)	660:752	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	2	25	theme	polymers	440:447	arg1	characterization					400:415	the structural characterization	385:415	the structural characterization of these heterogeneous polymers in cells	385:456	Their carbohydrate components are also promising candidates for bioenergy production and bioremediation, but the structural characterization of these heterogeneous polymers in cells remains a formidable problem.					
34735142	7	26	theme	important	1544:1552	arg1	species					1560:1566	other economically important algae species	1525:1566	other economically important algae species	1525:1566	These structural insights correlate with the high digestibility of this cellulose-deficient strain, and the in-cell ssNMR methods will facilitate the investigations of other economically important algae species.					
34735142	4	27	theme	cellulose-deficient	945:963	arg1	strain					965:970	a naturally cellulose-deficient strain	933:970	a naturally cellulose-deficient strain	933:970	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	3	28	theme	ssNMR	634:638	arg1	spectroscopy					641:652	magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	590:652	magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	590:652	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	4	29	theme	Parachlorella	848:860	arg1	beijerinckii					862:873	a uniformly 13C-labeled green microalga Parachlorella beijerinckii	808:873	a uniformly 13C-labeled green microalga Parachlorella beijerinckii	808:873	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	2	30	theme	heterogeneous	426:438	arg1	polymers					440:447	these heterogeneous polymers	420:447	these heterogeneous polymers	420:447	Their carbohydrate components are also promising candidates for bioenergy production and bioremediation, but the structural characterization of these heterogeneous polymers in cells remains a formidable problem.					
34735142	7	31	theme	strain	1449:1454	arg1	digestibility					1407:1419	the high digestibility	1398:1419	the high digestibility of this cellulose-deficient strain	1398:1454	These structural insights correlate with the high digestibility of this cellulose-deficient strain, and the in-cell ssNMR methods will facilitate the investigations of other economically important algae species.					
34735142	0	32	theme	Magnetic	137:144	arg1	Resonance					146:154	Solid-State Nuclear Magnetic Resonance	117:154	Solid-State Nuclear Magnetic Resonance	117:154	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	6	33	from	distribution	1314:1325	arg1	glycolipids					1330:1340	glycolipids	1330:1340	glycolipids	1330:1340	Surprisingly, most other carbohydrates are largely mobile, regardless of their distribution in glycolipids or cell walls.					
34735142	6	33	from	distribution	1314:1325	arg1	walls					1350:1354	cell walls	1345:1354	cell walls	1345:1354	Surprisingly, most other carbohydrates are largely mobile, regardless of their distribution in glycolipids or cell walls.					
34735142	2	34	theme	bioenergy	340:348	arg1	production					350:359	bioenergy production	340:359	bioenergy production	340:359	Their carbohydrate components are also promising candidates for bioenergy production and bioremediation, but the structural characterization of these heterogeneous polymers in cells remains a formidable problem.					
34735142	5	35	theme	rigid	1102:1106	arg1	domains					1108:1114	both the mobile and rigid domains	1082:1114	domains	1108:1114	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	5	35	theme	rigid	1102:1106	arg1	wall					1128:1131	the cell wall	1119:1131	the cell wall	1119:1131	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	3	36	theme	applicable	513:522	arg1	protocol					524:531	a widely applicable protocol	504:531	a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	504:652	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	0	37	theme	Nuclear	129:135	arg1	Resonance					146:154	Solid-State Nuclear Magnetic Resonance	117:154	Solid-State Nuclear Magnetic Resonance	117:154	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	3	38	theme	glycan	569:574	arg1	content					576:582	the glycan content	565:582	the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	565:652	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	2	39	theme	formidable	468:477	arg1	problem					479:485	a formidable problem	466:485	a formidable problem	466:485	Their carbohydrate components are also promising candidates for bioenergy production and bioremediation, but the structural characterization of these heterogeneous polymers in cells remains a formidable problem.					
34735142	5	40	with	present	1071:1077	arg1	shifts					1154:1159	their chemical shifts	1139:1159	their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants	1139:1232	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	5	41	attach	present	1071:1077	arg1	domains					1108:1114	both the mobile and rigid domains	1082:1114	domains	1108:1114	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	5	41	attach	present	1071:1077	arg2	xyloses					1059:1065	Some xyloses	1054:1065	Some xyloses	1054:1065	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	5	41	attach	present	1071:1077	arg1	wall					1128:1131	the cell wall	1119:1131	the cell wall	1119:1131	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	4	42	theme	Two-dimensional	755:769	arg1	spectra					797:803	Two-dimensional 13C-13C correlation ssNMR spectra	755:803	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii	755:873	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	4	43	from	structure	1031:1039	arg1	cell					1048:1051	the cell	1044:1051	the cell	1044:1051	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	4	44	theme	abundant	906:913	arg1	polysaccharide					915:928	the most abundant polysaccharide	897:928	the most abundant polysaccharide in a naturally cellulose-deficient strain	897:970	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	4	44	theme	abundant	906:913	arg1	starch					887:892	starch	887:892	starch	887:892	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	5	45	from	domains	1108:1114	arg1	present					1071:1077	present	1071:1077	present	1071:1077	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	7	46	theme	algae	1554:1558	arg1	species					1560:1566	other economically important algae species	1525:1566	other economically important algae species	1525:1566	These structural insights correlate with the high digestibility of this cellulose-deficient strain, and the in-cell ssNMR methods will facilitate the investigations of other economically important algae species.					
34735142	0	47	theme	Glycans	37:43	arg1	Quantification					19:32	Quantification	19:32	Quantification	19:32	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	0	47	theme	Glycans	37:43	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	7	48	theme	ssNMR	1473:1477	arg1	methods					1479:1485	the in-cell ssNMR methods	1461:1485	the in-cell ssNMR methods	1461:1485	These structural insights correlate with the high digestibility of this cellulose-deficient strain, and the in-cell ssNMR methods will facilitate the investigations of other economically important algae species.					
34735142	4	49	theme	green	832:836	arg1	beijerinckii					862:873	a uniformly 13C-labeled green microalga Parachlorella beijerinckii	808:873	a uniformly 13C-labeled green microalga Parachlorella beijerinckii	808:873	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	3	50	theme	composition	697:707	arg1	analysis					709:716	composition analysis	697:716	composition analysis	697:716	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	7	51	theme	in-cell	1465:1471	arg1	methods					1479:1485	the in-cell ssNMR methods	1461:1485	the in-cell ssNMR methods	1461:1485	These structural insights correlate with the high digestibility of this cellulose-deficient strain, and the in-cell ssNMR methods will facilitate the investigations of other economically important algae species.					
34735142	4	52	from	polysaccharide	915:928	arg1	strain					965:970	a naturally cellulose-deficient strain	933:970	a naturally cellulose-deficient strain	933:970	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	4	53	theme	ssNMR	791:795	arg1	spectra					797:803	Two-dimensional 13C-13C correlation ssNMR spectra	755:803	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii	755:873	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	0	54	theme	Whole	48:52	arg1	Cells					54:58	Whole Cells	48:58	Whole Cells	48:58	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	4	55	theme	rigid	1025:1029	arg1	structure					1031:1039	a well-organized and highly rigid structure	997:1039	a well-organized and highly rigid structure in the cell	997:1051	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	5	56	theme	cell	1123:1126	arg1	wall					1128:1131	the cell wall	1119:1131	the cell wall	1119:1131	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	5	57	theme	chemical	1145:1152	arg1	shifts					1154:1159	their chemical shifts	1139:1159	their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants	1139:1232	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	7	58	theme	cellulose-deficient	1429:1447	arg1	strain					1449:1454	this cellulose-deficient strain	1424:1454	this cellulose-deficient strain	1424:1454	These structural insights correlate with the high digestibility of this cellulose-deficient strain, and the in-cell ssNMR methods will facilitate the investigations of other economically important algae species.					
34735142	4	59	theme	correlation	779:789	arg1	spectra					797:803	Two-dimensional 13C-13C correlation ssNMR spectra	755:803	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii	755:873	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	5	60	theme	wall	1128:1131	arg1	domains					1108:1114	both the mobile and rigid domains	1082:1114	domains	1108:1114	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	5	60	theme	wall	1128:1131	arg1	wall					1128:1131	the cell wall	1119:1131	the cell wall	1119:1131	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	3	61	theme	glycosyl	676:683	arg1	linkage					685:691	glycosyl linkage	676:691	glycosyl linkage	676:691	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	7	62	theme	other	1525:1529	arg1	species					1560:1566	other economically important algae species	1525:1566	other economically important algae species	1525:1566	These structural insights correlate with the high digestibility of this cellulose-deficient strain, and the in-cell ssNMR methods will facilitate the investigations of other economically important algae species.					
34735142	3	63	theme	solid-state	617:627	arg1	spectroscopy					641:652	magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	590:652	magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy	590:652	Here we present a widely applicable protocol for identifying and quantifying the glycan content using magic-angle-spinning (MAS) solid-state NMR (ssNMR) spectroscopy, with validation from glycosyl linkage and composition analysis deduced from mass-spectrometry (MS).					
34735142	1	64	theme	sustainable	241:251	arg1	source					253:258	a sustainable source	239:258	a sustainable source of bioproducts	239:273	Microalgae are photosynthetic organisms widely distributed in nature and serve as a sustainable source of bioproducts.					
34735142	1	64	theme	sustainable	241:251	arg1	Microalgae					157:166	Microalgae	157:166	Microalgae	157:166	Microalgae are photosynthetic organisms widely distributed in nature and serve as a sustainable source of bioproducts.					
34735142	4	65	theme	well-organized	999:1012	arg1	structure					1031:1039	a well-organized and highly rigid structure	997:1039	a well-organized and highly rigid structure in the cell	997:1051	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	2	66	theme	carbohydrate	282:293	arg1	candidates					325:334	promising candidates	315:334	promising candidates for bioenergy production and bioremediation	315:378	Their carbohydrate components are also promising candidates for bioenergy production and bioremediation, but the structural characterization of these heterogeneous polymers in cells remains a formidable problem.					
34735142	2	66	theme	carbohydrate	282:293	arg1	components					295:304	Their carbohydrate components	276:304	Their carbohydrate components	276:304	Their carbohydrate components are also promising candidates for bioenergy production and bioremediation, but the structural characterization of these heterogeneous polymers in cells remains a formidable problem.					
34735142	4	67	theme	13C-labeled	820:830	arg1	beijerinckii					862:873	a uniformly 13C-labeled green microalga Parachlorella beijerinckii	808:873	a uniformly 13C-labeled green microalga Parachlorella beijerinckii	808:873	Two-dimensional 13C-13C correlation ssNMR spectra of a uniformly 13C-labeled green microalga Parachlorella beijerinckii reveal that starch is the most abundant polysaccharide in a naturally cellulose-deficient strain, and this polymer adopts a well-organized and highly rigid structure in the cell.					
34735142	5	68	theme	mobile	1091:1096	arg1	domains					1108:1114	both the mobile and rigid domains	1082:1114	domains	1108:1114	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	5	68	theme	mobile	1091:1096	arg1	wall					1128:1131	the cell wall	1119:1131	the cell wall	1119:1131	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	6	69	theme	other	1254:1258	arg1	carbohydrates					1260:1272	most other carbohydrates	1249:1272	most other carbohydrates	1249:1272	Surprisingly, most other carbohydrates are largely mobile, regardless of their distribution in glycolipids or cell walls.					
34735142	5	70	theme	2-fold	1200:1205	arg1	xylan					1207:1211	the flat-ribbon 2-fold xylan	1184:1211	the flat-ribbon 2-fold xylan identified in plants	1184:1232	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	0	71	dep	Identification	0:13	arg1	Architecture					61:72	Architecture	61:72	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.	0:155	Identification and Quantification of Glycans in Whole Cells: Architecture of Microalgal Polysaccharides Described by Solid-State Nuclear Magnetic Resonance.					
34735142	5	72	from	present	1071:1077	arg1	domains					1108:1114	both the mobile and rigid domains	1082:1114	domains	1108:1114	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	5	72	from	present	1071:1077	arg1	wall					1128:1131	the cell wall	1119:1131	the cell wall	1119:1131	Some xyloses are present in both the mobile and rigid domains of the cell wall, with their chemical shifts partially aligned with the flat-ribbon 2-fold xylan identified in plants.					
34735142	1	73	theme	bioproducts	263:273	arg1	source					253:258	a sustainable source	239:258	a sustainable source of bioproducts	239:273	Microalgae are photosynthetic organisms widely distributed in nature and serve as a sustainable source of bioproducts.					
34735142	1	73	theme	bioproducts	263:273	arg1	Microalgae					157:166	Microalgae	157:166	Microalgae	157:166	Microalgae are photosynthetic organisms widely distributed in nature and serve as a sustainable source of bioproducts.					
32321401	6	0	theme	lung	983:986	arg1	LC					996:997	LC	996:997	LC	996:997	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity were all analyzed.					
32321401	6	0	theme	lung	983:986	arg1	cancer					988:993	lung cancer	983:993	lung cancer (LC)	983:998	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity were all analyzed.					
32321401	1	1	theme	serum	260:264	arg1	glycoproteome					266:278	the human serum glycoproteome	250:278	the human serum glycoproteome	250:278	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	9	2	from	Decrease	1299:1306	arg1	structures					1390:1399	the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures	1311:1399	the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures	1311:1399	Decrease in the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures differentiated all disease groups from the control.					
32321401	12	3	theme	specific	1958:1965	arg1	potential					1988:1996	glycoprotein specific molecular diagnostic potential	1945:1996	glycoprotein specific molecular diagnostic potential	1945:1996	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	6	4	from	patients	963:970	arg1	samples					950:956	Pooled serum samples	937:956	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity	937:1020	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity were all analyzed.					
32321401	7	5	theme	standard	1143:1150	arg1	mixture					1160:1166	the standard protein mixture	1139:1166	the standard protein mixture	1139:1166	RESULTS Nine significant (>1%) asialo-N-glycan structures were identified both in human serum and the standard protein mixture.					
32321401	11	6	theme	disease	1718:1724	arg1	types					1726:1730	all three disease types	1708:1730	all three disease types	1708:1730	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	12	7	theme	desialylated	1906:1917	arg1	Nglycome					1931:1938	the desialylated human serum Nglycome	1902:1938	the desialylated human serum Nglycome	1902:1938	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	12	8	gly	glycoprotein	1945:1956	arg1	glycoprotein					1945:1956	glycoprotein specific molecular diagnostic potential	1945:1996	glycoprotein specific molecular diagnostic potential	1945:1996	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	12	9	theme	serum	1925:1929	arg1	Nglycome					1931:1938	the desialylated human serum Nglycome	1902:1938	the desialylated human serum Nglycome	1902:1938	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	7	10	theme	human	1123:1127	arg1	serum					1129:1133	human serum	1123:1133	human serum	1123:1133	RESULTS Nine significant (>1%) asialo-N-glycan structures were identified both in human serum and the standard protein mixture.					
32321401	3	11	theme	five-protein	579:590	arg1	model					592:596	a five-protein model	577:596	a five-protein model to deconstruct the overall N-glycosylation fingerprints in inflammatory and malignant lung diseases	577:696	OBJECTIVE Creating a five-protein model to deconstruct the overall N-glycosylation fingerprints in inflammatory and malignant lung diseases.					
32321401	8	12	theme	comorbidity	1279:1289	arg1	groups					1291:1296	the comorbidity groups	1275:1296	the comorbidity groups	1275:1296	The core-fucosylated-agalacto-biantennary glycan differentiated COPD and LC and both from the control and the comorbidity groups.					
32321401	0	13	from	Applications	76:87	arg1	Diseases					124:131	Inflammatory and Malignant Lung Diseases	92:131	Inflammatory and Malignant Lung Diseases	92:131	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	3	14	theme	N-glycosylation	625:639	arg1	fingerprints					641:652	the overall N-glycosylation fingerprints	613:652	the overall N-glycosylation fingerprints in inflammatory and malignant lung diseases	613:696	OBJECTIVE Creating a five-protein model to deconstruct the overall N-glycosylation fingerprints in inflammatory and malignant lung diseases.					
32321401	12	15	from	changes	1891:1897	arg1	Nglycome					1931:1938	the desialylated human serum Nglycome	1902:1938	the desialylated human serum Nglycome	1902:1938	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	7	16	theme	significant	1054:1064	arg1	structures					1088:1097	Nine significant (>1%) asialo-N-glycan structures	1049:1097	Nine significant (>1%) asialo-N-glycan structures	1049:1097	RESULTS Nine significant (>1%) asialo-N-glycan structures were identified both in human serum and the standard protein mixture.					
32321401	4	17	theme	abundant	758:765	arg1	glycoproteins					773:785	the five high abundant serum glycoproteins	744:785	the five high abundant serum glycoproteins	744:785	METHODS The N-glycan pool of human serum and the five high abundant serum glycoproteins were analyzed.					
32321401	7	18	theme	asialo-N-glycan	1072:1086	arg1	structures					1088:1097	Nine significant (>1%) asialo-N-glycan structures	1049:1097	Nine significant (>1%) asialo-N-glycan structures	1049:1097	RESULTS Nine significant (>1%) asialo-N-glycan structures were identified both in human serum and the standard protein mixture.					
32321401	1	19	theme	Immunoglobulin	145:158	arg1	G					160:160	BACKGROUND Immunoglobulin G	134:160	BACKGROUND Immunoglobulin G	134:160	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	4	20	theme	human	728:732	arg1	serum					734:738	human serum	728:738	human serum	728:738	METHODS The N-glycan pool of human serum and the five high abundant serum glycoproteins were analyzed.					
32321401	5	21	theme	Simultaneous	802:813	arg1	digestion					841:849	Simultaneous endoglycosidase/sialidase digestion	802:849	Simultaneous endoglycosidase/sialidase digestion	802:849	Simultaneous endoglycosidase/sialidase digestion was followed by fluorophore labeling and separation by CE-LIF to establish the model.					
32321401	2	22	theme	N-glycoproteome	481:495	arg1	subset					461:466	the desialylated subset	444:466	the desialylated subset of the serum N-glycoproteome	444:495	However, due to the labile nature of the sialic acid residues, the desialylated subset of the serum N-glycoproteome has been traditionally utilized for diagnostic applications.					
32321401	1	23	theme	molecular	354:362	arg1	markers					372:378	important molecular disease markers	344:378	important molecular disease markers	344:378	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	2	24	theme	diagnostic	533:542	arg1	applications					544:555	diagnostic applications	533:555	diagnostic applications	533:555	However, due to the labile nature of the sialic acid residues, the desialylated subset of the serum N-glycoproteome has been traditionally utilized for diagnostic applications.					
32321401	11	25	located	observed	1808:1815	arg1	comparison					1820:1829	comparison	1820:1829	comparison to the control group	1820:1850	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	11	25	located	observed	1808:1815	arg2	increase					1639:1646	some increase	1634:1646	some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC	1634:1801	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	0	26	theme	Inflammatory	92:103	arg1	Diseases					124:131	Inflammatory and Malignant Lung Diseases	92:131	Inflammatory and Malignant Lung Diseases	92:131	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	1	27	theme	markers	372:378	arg1	discovery					331:339	the discovery	327:339	the discovery of important molecular disease markers	327:378	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	2	28	theme	labile	401:406	arg1	nature					408:413	the labile nature	397:413	the labile nature of the sialic acid residues	397:441	However, due to the labile nature of the sialic acid residues, the desialylated subset of the serum N-glycoproteome has been traditionally utilized for diagnostic applications.					
32321401	0	29	theme	Malignant	109:117	arg1	Diseases					124:131	Inflammatory and Malignant Lung Diseases	92:131	Inflammatory and Malignant Lung Diseases	92:131	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	2	30	theme	residues	434:441	arg1	nature					408:413	the labile nature	397:413	the labile nature of the sialic acid residues	397:441	However, due to the labile nature of the sialic acid residues, the desialylated subset of the serum N-glycoproteome has been traditionally utilized for diagnostic applications.					
32321401	12	31	dep	CONCLUSION	1853:1862	arg1	suggested					1876:1884	suggested	1876:1884	suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture	1876:2093	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	2	32	theme	sialic	422:427	arg1	residues					434:441	the sialic acid residues	418:441	the sialic acid residues	418:441	However, due to the labile nature of the sialic acid residues, the desialylated subset of the serum N-glycoproteome has been traditionally utilized for diagnostic applications.					
32321401	6	33	theme	serum	944:948	arg1	samples					950:956	Pooled serum samples	937:956	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity	937:1020	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity were all analyzed.					
32321401	0	34	theme	Molecular	55:63	arg1	Applications					76:87	Molecular Diagnostic Applications	55:87	Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases	55:131	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	2	35	used	utilized	520:527	arg2	subset					461:466	the desialylated subset	444:466	the desialylated subset of the serum N-glycoproteome	444:495	However, due to the labile nature of the sialic acid residues, the desialylated subset of the serum N-glycoproteome has been traditionally utilized for diagnostic applications.					
32321401	0	36	theme	Human	29:33	arg1	N-glycome					41:49	the Desialylated Human Serum N-glycome	12:49	the Desialylated Human Serum N-glycome	12:49	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	12	37	theme	inflammatory	2016:2027	arg1	diseases					2034:2041	malignant and inflammatory lung diseases	2002:2041	malignant and inflammatory lung diseases	2002:2041	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	3	38	theme	lung	684:687	arg1	diseases					689:696	malignant lung diseases	674:696	malignant lung diseases	674:696	OBJECTIVE Creating a five-protein model to deconstruct the overall N-glycosylation fingerprints in inflammatory and malignant lung diseases.					
32321401	10	39	theme	significant	1457:1467	arg1	specific					1546:1553	specific	1546:1553	specific	1546:1553	The significant increase of the fucosylated-galactosylated-triantennary structure was highly specific for LC, to a medium extent for COPD and a lesser extent for comorbidity.					
32321401	10	39	theme	significant	1457:1467	arg1	increase					1469:1476	The significant increase	1453:1476	The significant increase of the fucosylated-galactosylated-triantennary structure	1453:1533	The significant increase of the fucosylated-galactosylated-triantennary structure was highly specific for LC, to a medium extent for COPD and a lesser extent for comorbidity.					
32321401	5	40	theme	fluorophore	867:877	arg1	labeling					879:886	fluorophore labeling	867:886	fluorophore labeling	867:886	Simultaneous endoglycosidase/sialidase digestion was followed by fluorophore labeling and separation by CE-LIF to establish the model.					
32321401	0	41	gly	Desialylated	16:27	arg1	N-glycome					41:49	the Desialylated Human Serum N-glycome	12:49	the Desialylated Human Serum N-glycome	12:49	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	11	42	theme	afucosylated-galactosylated-triantennary	1736:1775	arg1	structures					1777:1786	afucosylated-galactosylated-triantennary structures	1736:1786	afucosylated-galactosylated-triantennary structures in COPD and LC	1736:1801	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	7	43	dep	identified	1104:1113	arg1	mixture					1160:1166	the standard protein mixture	1139:1166	the standard protein mixture	1139:1166	RESULTS Nine significant (>1%) asialo-N-glycan structures were identified both in human serum and the standard protein mixture.					
32321401	7	43	dep	identified	1104:1113	arg1	both					1115:1118	both	1115:1118	both	1115:1118	RESULTS Nine significant (>1%) asialo-N-glycan structures were identified both in human serum and the standard protein mixture.					
32321401	10	44	theme	fucosylated-galactosylated-triantennary	1485:1523	arg1	structure					1525:1533	the fucosylated-galactosylated-triantennary structure	1481:1533	the fucosylated-galactosylated-triantennary structure	1481:1533	The significant increase of the fucosylated-galactosylated-triantennary structure was highly specific for LC, to a medium extent for COPD and a lesser extent for comorbidity.					
32321401	8	45	theme	core-fucosylated-agalacto-biantennary	1173:1209	arg1	glycan					1211:1216	The core-fucosylated-agalacto-biantennary glycan	1169:1216	The core-fucosylated-agalacto-biantennary glycan	1169:1216	The core-fucosylated-agalacto-biantennary glycan differentiated COPD and LC and both from the control and the comorbidity groups.					
32321401	12	46	theme	malignant	2002:2010	arg1	diseases					2034:2041	malignant and inflammatory lung diseases	2002:2041	malignant and inflammatory lung diseases	2002:2041	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	1	47	theme	human	254:258	arg1	glycoproteome					266:278	the human serum glycoproteome	250:278	the human serum glycoproteome	250:278	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	12	48	theme	glycoprotein	1945:1956	arg1	potential					1988:1996	glycoprotein specific molecular diagnostic potential	1945:1996	glycoprotein specific molecular diagnostic potential	1945:1996	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	6	49	with	patients	963:970	arg1	comorbidity					1010:1020	their comorbidity	1004:1020	their comorbidity	1004:1020	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity were all analyzed.					
32321401	6	49	with	patients	963:970	arg1	LC					996:997	LC	996:997	LC	996:997	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity were all analyzed.					
32321401	6	49	with	patients	963:970	arg1	COPD					977:980	COPD	977:980	COPD	977:980	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity were all analyzed.					
32321401	6	49	with	patients	963:970	arg1	cancer					988:993	lung cancer	983:993	lung cancer (LC)	983:998	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity were all analyzed.					
32321401	7	50	theme	protein	1152:1158	arg1	mixture					1160:1166	the standard protein mixture	1139:1166	the standard protein mixture	1139:1166	RESULTS Nine significant (>1%) asialo-N-glycan structures were identified both in human serum and the standard protein mixture.					
32321401	1	51	theme	glycoproteome	266:278	arg1	glycoproteome					266:278	the human serum glycoproteome	250:278	the human serum glycoproteome	250:278	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	1	51	theme	glycoproteome	266:278	arg1	%					245:245	approximately 85%	229:245	approximately 85% of the human serum glycoproteome	229:278	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	12	52	theme	molecular	1967:1975	arg1	potential					1988:1996	glycoprotein specific molecular diagnostic potential	1945:1996	glycoprotein specific molecular diagnostic potential	1945:1996	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	11	53	theme	afucosylated-galactosylated-biantennary	1655:1693	arg1	structure					1695:1703	the afucosylated-galactosylated-biantennary structure	1651:1703	the afucosylated-galactosylated-biantennary structure in all three disease types	1651:1730	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	0	54	theme	N-glycome	41:49	arg1	Modeling					0:7	Modeling	0:7	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.	0:132	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	12	55	theme	human	1919:1923	arg1	Nglycome					1931:1938	the desialylated human serum Nglycome	1902:1938	the desialylated human serum Nglycome	1902:1938	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	11	56	theme	control	1838:1844	arg1	group					1846:1850	the control group	1834:1850	the control group	1834:1850	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	4	57	theme	N-glycan	711:718	arg1	pool					720:723	The N-glycan pool	707:723	The N-glycan pool of human serum	707:738	METHODS The N-glycan pool of human serum and the five high abundant serum glycoproteins were analyzed.					
32321401	1	58	theme	alpha-1-	198:205	arg1	antitrypsin					207:217	alpha-1- antitrypsin	198:217	alpha-1- antitrypsin	198:217	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	12	59	gly	desialylated	1906:1917	arg1	Nglycome					1931:1938	the desialylated human serum Nglycome	1902:1938	the desialylated human serum Nglycome	1902:1938	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	3	60	theme	overall	617:623	arg1	fingerprints					641:652	the overall N-glycosylation fingerprints	613:652	the overall N-glycosylation fingerprints in inflammatory and malignant lung diseases	613:696	OBJECTIVE Creating a five-protein model to deconstruct the overall N-glycosylation fingerprints in inflammatory and malignant lung diseases.					
32321401	11	61	from	structures	1777:1786	arg1	LC					1800:1801	LC	1800:1801	LC	1800:1801	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	11	61	from	structures	1777:1786	arg1	COPD					1791:1794	COPD	1791:1794	COPD	1791:1794	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	11	61	from	structures	1777:1786	arg1	types					1726:1730	all three disease types	1708:1730	all three disease types	1708:1730	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	9	62	theme	core-fucosylated-agalacto-biantennary-bisecting	1315:1361	arg1	structures					1390:1399	the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures	1311:1399	the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures	1311:1399	Decrease in the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures differentiated all disease groups from the control.					
32321401	9	63	theme	disease	1420:1426	arg1	groups					1428:1433	all disease groups	1416:1433	all disease groups	1416:1433	Decrease in the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures differentiated all disease groups from the control.					
32321401	9	64	theme	monogalacto	1364:1374	arg1	structures					1390:1399	the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures	1311:1399	the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures	1311:1399	Decrease in the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures differentiated all disease groups from the control.					
32321401	1	65	theme	BACKGROUND	134:143	arg1	G					160:160	BACKGROUND Immunoglobulin G	134:160	BACKGROUND Immunoglobulin G	134:160	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	4	66	theme	high	753:756	arg1	glycoproteins					773:785	the five high abundant serum glycoproteins	744:785	the five high abundant serum glycoproteins	744:785	METHODS The N-glycan pool of human serum and the five high abundant serum glycoproteins were analyzed.					
32321401	0	67	theme	Diagnostic	65:74	arg1	Applications					76:87	Molecular Diagnostic Applications	55:87	Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases	55:131	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	9	68	theme	bigalacto	1380:1388	arg1	structures					1390:1399	the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures	1311:1399	the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures	1311:1399	Decrease in the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures differentiated all disease groups from the control.					
32321401	4	69	gly	glycoproteins	773:785	arg1	glycoproteins					773:785	the five high abundant serum glycoproteins	744:785	the five high abundant serum glycoproteins	744:785	METHODS The N-glycan pool of human serum and the five high abundant serum glycoproteins were analyzed.					
32321401	5	70	theme	endoglycosidase/sialidase	815:839	arg1	digestion					841:849	Simultaneous endoglycosidase/sialidase digestion	802:849	Simultaneous endoglycosidase/sialidase digestion	802:849	Simultaneous endoglycosidase/sialidase digestion was followed by fluorophore labeling and separation by CE-LIF to establish the model.					
32321401	1	71	theme	important	344:352	arg1	markers					372:378	important molecular disease markers	344:378	important molecular disease markers	344:378	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	11	72	from	structure	1695:1703	arg1	LC					1800:1801	LC	1800:1801	LC	1800:1801	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	11	72	from	structure	1695:1703	arg1	COPD					1791:1794	COPD	1791:1794	COPD	1791:1794	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	11	72	from	structure	1695:1703	arg1	types					1726:1730	all three disease types	1708:1730	all three disease types	1708:1730	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	2	73	theme	serum	475:479	arg1	N-glycoproteome					481:495	the serum N-glycoproteome	471:495	the serum N-glycoproteome	471:495	However, due to the labile nature of the sialic acid residues, the desialylated subset of the serum N-glycoproteome has been traditionally utilized for diagnostic applications.					
32321401	4	74	theme	serum	734:738	arg1	pool					720:723	The N-glycan pool	707:723	The N-glycan pool of human serum	707:738	METHODS The N-glycan pool of human serum and the five high abundant serum glycoproteins were analyzed.					
32321401	1	75	theme	disease	364:370	arg1	markers					372:378	important molecular disease markers	344:378	important molecular disease markers	344:378	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	10	76	theme	medium	1568:1573	arg1	extent					1575:1580	a medium extent	1566:1580	a medium extent for COPD	1566:1589	The significant increase of the fucosylated-galactosylated-triantennary structure was highly specific for LC, to a medium extent for COPD and a lesser extent for comorbidity.					
32321401	3	77	from	fingerprints	641:652	arg1	diseases					689:696	malignant lung diseases	674:696	malignant lung diseases	674:696	OBJECTIVE Creating a five-protein model to deconstruct the overall N-glycosylation fingerprints in inflammatory and malignant lung diseases.					
32321401	3	77	from	fingerprints	641:652	arg1	inflammatory					657:668	inflammatory	657:668	inflammatory	657:668	OBJECTIVE Creating a five-protein model to deconstruct the overall N-glycosylation fingerprints in inflammatory and malignant lung diseases.					
32321401	0	78	theme	Lung	119:122	arg1	Diseases					124:131	Inflammatory and Malignant Lung Diseases	92:131	Inflammatory and Malignant Lung Diseases	92:131	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	7	79	dep	significant	1054:1064	arg1	%					1069:1069	>1%	1067:1069	>1%	1067:1069	RESULTS Nine significant (>1%) asialo-N-glycan structures were identified both in human serum and the standard protein mixture.					
32321401	11	80	from	increase	1639:1646	arg1	structures					1777:1786	afucosylated-galactosylated-triantennary structures	1736:1786	afucosylated-galactosylated-triantennary structures in COPD and LC	1736:1801	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	11	80	from	increase	1639:1646	arg1	structure					1695:1703	the afucosylated-galactosylated-biantennary structure	1651:1703	the afucosylated-galactosylated-biantennary structure in all three disease types	1651:1730	Also, some increase in the afucosylated-galactosylated-biantennary structure in all three disease types and afucosylated-galactosylated-triantennary structures in COPD and LC were observed in comparison to the control group.					
32321401	10	81	theme	lesser	1597:1602	arg1	extent					1604:1609	a lesser extent	1595:1609	a lesser extent for comorbidity	1595:1625	The significant increase of the fucosylated-galactosylated-triantennary structure was highly specific for LC, to a medium extent for COPD and a lesser extent for comorbidity.					
32321401	4	82	theme	serum	767:771	arg1	glycoproteins					773:785	the five high abundant serum glycoproteins	744:785	the five high abundant serum glycoproteins	744:785	METHODS The N-glycan pool of human serum and the five high abundant serum glycoproteins were analyzed.					
32321401	0	83	theme	Desialylated	16:27	arg1	N-glycome					41:49	the Desialylated Human Serum N-glycome	12:49	the Desialylated Human Serum N-glycome	12:49	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	1	84	theme	N-glycosylation	290:304	arg1	analysis					306:313	their N-glycosylation analysis	284:313	their N-glycosylation analysis	284:313	BACKGROUND Immunoglobulin G and A, transferrin, haptoglobin and alpha-1- antitrypsin represent approximately 85% of the human serum glycoproteome and their N-glycosylation analysis may lead to the discovery of important molecular disease markers.					
32321401	2	85	theme	acid	429:432	arg1	residues					434:441	the sialic acid residues	418:441	the sialic acid residues	418:441	However, due to the labile nature of the sialic acid residues, the desialylated subset of the serum N-glycoproteome has been traditionally utilized for diagnostic applications.					
32321401	7	86	dep	RESULTS	1041:1047	arg1	identified					1104:1113	identified	1104:1113	were identified both in human serum and the standard protein mixture	1099:1166	RESULTS Nine significant (>1%) asialo-N-glycan structures were identified both in human serum and the standard protein mixture.					
32321401	0	87	theme	Serum	35:39	arg1	N-glycome					41:49	the Desialylated Human Serum N-glycome	12:49	the Desialylated Human Serum N-glycome	12:49	Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases.					
32321401	12	88	theme	five-protein	2074:2085	arg1	mixture					2087:2093	the five-protein mixture	2070:2093	the five-protein mixture	2070:2093	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	6	89	theme	Pooled	937:942	arg1	samples					950:956	Pooled serum samples	937:956	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity	937:1020	Pooled serum samples from patients with COPD, lung cancer (LC) and their comorbidity were all analyzed.					
32321401	3	90	theme	malignant	674:682	arg1	diseases					689:696	malignant lung diseases	674:696	malignant lung diseases	674:696	OBJECTIVE Creating a five-protein model to deconstruct the overall N-glycosylation fingerprints in inflammatory and malignant lung diseases.					
32321401	2	91	gly	desialylated	448:459	arg1	subset					461:466	the desialylated subset	444:466	the desialylated subset of the serum N-glycoproteome	444:495	However, due to the labile nature of the sialic acid residues, the desialylated subset of the serum N-glycoproteome has been traditionally utilized for diagnostic applications.					
32321401	2	92	theme	desialylated	448:459	arg1	subset					461:466	the desialylated subset	444:466	the desialylated subset of the serum N-glycoproteome	444:495	However, due to the labile nature of the sialic acid residues, the desialylated subset of the serum N-glycoproteome has been traditionally utilized for diagnostic applications.					
32321401	9	93	dep	groups	1428:1433	arg1	control					1444:1450	control	1444:1450	control	1444:1450	Decrease in the core-fucosylated-agalacto-biantennary-bisecting, monogalacto and bigalacto structures differentiated all disease groups from the control.					
32321401	12	94	theme	lung	2029:2032	arg1	diseases					2034:2041	malignant and inflammatory lung diseases	2002:2041	malignant and inflammatory lung diseases	2002:2041	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	10	95	theme	structure	1525:1533	arg1	specific					1546:1553	specific	1546:1553	specific	1546:1553	The significant increase of the fucosylated-galactosylated-triantennary structure was highly specific for LC, to a medium extent for COPD and a lesser extent for comorbidity.					
32321401	10	95	theme	structure	1525:1533	arg1	increase					1469:1476	The significant increase	1453:1476	The significant increase of the fucosylated-galactosylated-triantennary structure	1453:1533	The significant increase of the fucosylated-galactosylated-triantennary structure was highly specific for LC, to a medium extent for COPD and a lesser extent for comorbidity.					
32321401	12	96	theme	diagnostic	1977:1986	arg1	potential					1988:1996	glycoprotein specific molecular diagnostic potential	1945:1996	glycoprotein specific molecular diagnostic potential	1945:1996	CONCLUSION Our results suggested that changes in the desialylated human serum Nglycome hold glycoprotein specific molecular diagnostic potential for malignant and inflammatory lung diseases, which can be modeled with the five-protein mixture.					
32321401	7	97	dep	both	1115:1118	arg1	serum					1129:1133	human serum	1123:1133	human serum	1123:1133	RESULTS Nine significant (>1%) asialo-N-glycan structures were identified both in human serum and the standard protein mixture.					
33196194	0	0	theme	Glycoproteomic	95:108	arg1	Approach					110:117	the LC-MS/MS-Based Glycoproteomic Approach	76:117	the LC-MS/MS-Based Glycoproteomic Approach	76:117	Analysis of NIST Monoclonal Antibody Reference Material Glycosylation Using the LC-MS/MS-Based Glycoproteomic Approach.					
33196194	6	1	theme	identified	862:871	arg1	glycans					873:879	the identified glycans	858:879	the identified glycans presented at levels smaller than 0.1%	858:917	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	6	2	theme	glycan	727:732	arg1	structure					734:742	The glycan structure	723:742	The glycan structure with the highest abundance	723:769	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	6	2	theme	glycan	727:732	arg1	FA2					775:777	FA2	775:777	FA2	775:777	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	10	3	located	observed	1298:1305	arg2	peptides					1279:1286	unglycosylated peptides	1264:1286	unglycosylated peptides	1264:1286	Moreover, unglycosylated peptides were also observed at a level of 1-2%.					
33196194	10	3	located	observed	1298:1305	arg1	level					1312:1316	a level	1310:1316	a level of 1-2%	1310:1324	Moreover, unglycosylated peptides were also observed at a level of 1-2%.					
33196194	5	4	theme	glycan	671:676	arg1	compositions					678:689	48 glycan compositions	668:689	48 glycan compositions	668:689	Overall, 48 glycan compositions were identified and quantified.					
33196194	4	5	theme	sample	595:600	arg1	B					602:602	sample B	595:602	sample B	595:602	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	4	5	theme	sample	595:600	arg1	8671					589:592	an NISTmAb reference material (RM) 8671	554:592	an NISTmAb reference material (RM) 8671 (sample B)	554:603	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	0	6	theme	LC-MS/MS-Based	80:93	arg1	Approach					110:117	the LC-MS/MS-Based Glycoproteomic Approach	76:117	the LC-MS/MS-Based Glycoproteomic Approach	76:117	Analysis of NIST Monoclonal Antibody Reference Material Glycosylation Using the LC-MS/MS-Based Glycoproteomic Approach.					
33196194	9	7	theme	sialylation	1209:1219	arg1	levels					1199:1204	the levels	1195:1204	the levels of sialylation, fucosylation, and high mannose	1195:1251	There was no significant difference between the two samples in the levels of sialylation, fucosylation, and high mannose.					
33196194	6	8	theme	%	811:811	arg1	abundance					796:804	a relative abundance	785:804	a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%	785:917	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	9	9	theme	significant	1145:1155	arg1	difference					1157:1166	no significant difference	1142:1166	no significant difference between the two samples in the levels of sialylation, fucosylation, and high mannose	1142:1251	There was no significant difference between the two samples in the levels of sialylation, fucosylation, and high mannose.					
33196194	6	10	with	structure	734:742	arg1	abundance					761:769	the highest abundance	749:769	the highest abundance	749:769	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	9	11	theme	high	1240:1243	arg1	mannose					1245:1251	high mannose	1240:1251	high mannose	1240:1251	There was no significant difference between the two samples in the levels of sialylation, fucosylation, and high mannose.					
33196194	10	12	theme	%	1324:1324	arg1	level					1312:1316	a level	1310:1316	a level of 1-2%	1310:1324	Moreover, unglycosylated peptides were also observed at a level of 1-2%.					
33196194	7	13	theme	glycan	930:935	arg1	attributes					937:946	Important glycan attributes	920:946	Important glycan attributes	920:946	Important glycan attributes were further derived using the quantitative results.					
33196194	8	14	theme	galactosylation	1098:1112	arg1	level					1114:1118	the galactosylation level	1094:1118	the galactosylation level of NISTmAb	1094:1129	The galactosylation level of modified NISTmAb was found to decrease by ∼10% when compared to the galactosylation level of NISTmAb.					
33196194	4	15	theme	glycoproteomics	499:513	arg1	approach					515:522	an LC-MS/MS-based glycoproteomics approach	481:522	an LC-MS/MS-based glycoproteomics approach	481:522	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	8	16	theme	NISTmAb	1123:1129	arg1	level					1114:1118	the galactosylation level	1094:1118	the galactosylation level of NISTmAb	1094:1129	The galactosylation level of modified NISTmAb was found to decrease by ∼10% when compared to the galactosylation level of NISTmAb.					
33196194	4	17	theme	LC-MS/MS-based	484:497	arg1	approach					515:522	an LC-MS/MS-based glycoproteomics approach	481:522	an LC-MS/MS-based glycoproteomics approach	481:522	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	6	18	with	FA2	775:777	arg1	abundance					796:804	a relative abundance	785:804	a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%	785:917	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	1	19	theme	modern	190:195	arg1	medicine					197:204	modern medicine	190:204	modern medicine	190:204	Protein-based therapeutics such as mAbs have become emerging drugs in modern medicine.					
33196194	6	20	from	A	823:823	arg1	abundance					796:804	a relative abundance	785:804	a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%	785:917	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	0	21	theme	Reference	37:45	arg1	Glycosylation					56:68	NIST Monoclonal Antibody Reference Material Glycosylation	12:68	NIST Monoclonal Antibody Reference Material Glycosylation	12:68	Analysis of NIST Monoclonal Antibody Reference Material Glycosylation Using the LC-MS/MS-Based Glycoproteomic Approach.					
33196194	6	22	theme	relative	787:794	arg1	abundance					796:804	a relative abundance	785:804	a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%	785:917	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	6	23	from	abundance	796:804	arg1	A					823:823	sample A	816:823	sample A	816:823	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	6	23	from	abundance	796:804	arg1	sample					836:841	sample B.	836:844	sample B.	836:844	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	6	24	from	%	831:831	arg1	A					823:823	sample A	816:823	sample A	816:823	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	6	24	from	%	831:831	arg1	sample					836:841	sample B.	836:844	sample B.	836:844	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	8	25	theme	modified	1030:1037	arg1	NISTmAb					1039:1045	modified NISTmAb	1030:1045	modified NISTmAb	1030:1045	The galactosylation level of modified NISTmAb was found to decrease by ∼10% when compared to the galactosylation level of NISTmAb.					
33196194	9	26	theme	mannose	1245:1251	arg1	levels					1199:1204	the levels	1195:1204	the levels of sialylation, fucosylation, and high mannose	1195:1251	There was no significant difference between the two samples in the levels of sialylation, fucosylation, and high mannose.					
33196194	6	27	from	%	811:811	arg1	A					823:823	sample A	816:823	sample A	816:823	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	6	27	from	%	811:811	arg1	sample					836:841	sample B.	836:844	sample B.	836:844	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	4	28	theme	RM	585:586	arg1	B					602:602	sample B	595:602	sample B	595:602	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	4	28	theme	RM	585:586	arg1	8671					589:592	an NISTmAb reference material (RM) 8671	554:592	an NISTmAb reference material (RM) 8671 (sample B)	554:603	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	3	29	theme	biotherapeutics	447:461	arg1	PK/PD					437:441	PK/PD	437:441	PK/PD	437:441	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	29	theme	biotherapeutics	447:461	arg1	efficacy					388:395	efficacy	388:395	efficacy	388:395	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	29	theme	biotherapeutics	447:461	arg1	pharmacokinetics/pharmacodynamics					402:434	pharmacokinetics/pharmacodynamics	402:434	pharmacokinetics/pharmacodynamics	402:434	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	29	theme	biotherapeutics	447:461	arg1	safety					380:385	safety	380:385	safety	380:385	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	0	30	theme	Monoclonal	17:26	arg1	Antibody					28:35	NIST Monoclonal Antibody	12:35	NIST Monoclonal Antibody Reference Material Glycosylation	12:68	Analysis of NIST Monoclonal Antibody Reference Material Glycosylation Using the LC-MS/MS-Based Glycoproteomic Approach.					
33196194	1	31	theme	Protein-based	120:132	arg1	mAbs					155:158	mAbs	155:158	mAbs	155:158	Protein-based therapeutics such as mAbs have become emerging drugs in modern medicine.					
33196194	1	31	theme	Protein-based	120:132	arg1	therapeutics					134:145	Protein-based therapeutics	120:145	Protein-based therapeutics such as mAbs	120:158	Protein-based therapeutics such as mAbs have become emerging drugs in modern medicine.					
33196194	4	32	theme	sample	648:653	arg1	A					655:655	sample A	648:655	sample A	648:655	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	4	32	theme	sample	648:653	arg1	NISTmAb					639:645	a β-1,4-galactosidase-treated NISTmAb	609:645	a β-1,4-galactosidase-treated NISTmAb (sample A)	609:656	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	0	33	theme	NIST	12:15	arg1	Antibody					28:35	NIST Monoclonal Antibody	12:35	NIST Monoclonal Antibody Reference Material Glycosylation	12:68	Analysis of NIST Monoclonal Antibody Reference Material Glycosylation Using the LC-MS/MS-Based Glycoproteomic Approach.					
33196194	4	34	theme	8671	589:592	arg1	glycans					543:549	Fc glycans	540:549	Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A)	540:656	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	8	35	theme	NISTmAb	1039:1045	arg1	level					1021:1025	The galactosylation level	1001:1025	The galactosylation level of modified NISTmAb	1001:1045	The galactosylation level of modified NISTmAb was found to decrease by ∼10% when compared to the galactosylation level of NISTmAb.					
33196194	10	36	gly	unglycosylated	1264:1277	arg1	peptides					1279:1286	unglycosylated peptides	1264:1286	unglycosylated peptides	1264:1286	Moreover, unglycosylated peptides were also observed at a level of 1-2%.					
33196194	9	37	theme	fucosylation	1222:1233	arg1	levels					1199:1204	the levels	1195:1204	the levels of sialylation, fucosylation, and high mannose	1195:1251	There was no significant difference between the two samples in the levels of sialylation, fucosylation, and high mannose.					
33196194	2	38	gly	glycoproteins	253:265	arg1	glycoproteins					253:265	glycoproteins	253:265	glycoproteins	253:265	Most of the approved therapeutic proteins are glycoproteins.					
33196194	2	38	gly	glycoproteins	253:265	arg1	proteins					240:247	the approved therapeutic proteins	215:247	the approved therapeutic proteins	215:247	Most of the approved therapeutic proteins are glycoproteins.					
33196194	2	38	gly	glycoproteins	253:265	arg1	Most					207:210	Most	207:210	Most	207:210	Most of the approved therapeutic proteins are glycoproteins.					
33196194	3	39	theme	critical	298:305	arg1	attribute					315:323	an essential critical quality attribute	285:323	an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics	285:461	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	39	theme	critical	298:305	arg1	Glycosylation					268:280	Glycosylation	268:280	Glycosylation	268:280	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	39	theme	critical	298:305	arg1	CQA					326:328	CQA	326:328	CQA	326:328	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	0	40	theme	Antibody	28:35	arg1	Glycosylation					56:68	NIST Monoclonal Antibody Reference Material Glycosylation	12:68	NIST Monoclonal Antibody Reference Material Glycosylation	12:68	Analysis of NIST Monoclonal Antibody Reference Material Glycosylation Using the LC-MS/MS-Based Glycoproteomic Approach.					
33196194	4	41	theme	NISTmAb	557:563	arg1	B					602:602	sample B	595:602	sample B	595:602	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	4	41	theme	NISTmAb	557:563	arg1	8671					589:592	an NISTmAb reference material (RM) 8671	554:592	an NISTmAb reference material (RM) 8671 (sample B)	554:603	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	3	42	theme	quality	307:313	arg1	attribute					315:323	an essential critical quality attribute	285:323	an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics	285:461	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	42	theme	quality	307:313	arg1	Glycosylation					268:280	Glycosylation	268:280	Glycosylation	268:280	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	42	theme	quality	307:313	arg1	CQA					326:328	CQA	326:328	CQA	326:328	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	6	43	theme	%	831:831	arg1	abundance					796:804	a relative abundance	785:804	a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%	785:917	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	6	44	from	sample	836:841	arg1	abundance					796:804	a relative abundance	785:804	a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%	785:917	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	9	45	from	samples	1184:1190	arg1	levels					1199:1204	the levels	1195:1204	the levels of sialylation, fucosylation, and high mannose	1195:1251	There was no significant difference between the two samples in the levels of sialylation, fucosylation, and high mannose.					
33196194	0	46	theme	Glycosylation	56:68	arg1	Analysis					0:7	Analysis	0:7	Analysis of NIST Monoclonal Antibody Reference Material Glycosylation	0:68	Analysis of NIST Monoclonal Antibody Reference Material Glycosylation Using the LC-MS/MS-Based Glycoproteomic Approach.					
33196194	6	47	theme	smaller	901:907	arg1	levels					894:899	levels	894:899	levels smaller than 0.1%	894:917	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	3	48	theme	due	331:333	arg1	attribute					315:323	an essential critical quality attribute	285:323	an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics	285:461	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	48	theme	due	331:333	arg1	Glycosylation					268:280	Glycosylation	268:280	Glycosylation	268:280	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	48	theme	due	331:333	arg1	CQA					326:328	CQA	326:328	CQA	326:328	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	49	contain	have	368:371	arg1	glycoforms					357:366	glycoforms	357:366	glycoforms	357:366	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	49	contain	have	368:371	arg2	influence					342:350	the influence	338:350	the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics	338:461	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	0	50	theme	Material	47:54	arg1	Glycosylation					56:68	NIST Monoclonal Antibody Reference Material Glycosylation	12:68	NIST Monoclonal Antibody Reference Material Glycosylation	12:68	Analysis of NIST Monoclonal Antibody Reference Material Glycosylation Using the LC-MS/MS-Based Glycoproteomic Approach.					
33196194	4	51	theme	material	575:582	arg1	B					602:602	sample B	595:602	sample B	595:602	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	4	51	theme	material	575:582	arg1	8671					589:592	an NISTmAb reference material (RM) 8671	554:592	an NISTmAb reference material (RM) 8671 (sample B)	554:603	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	6	52	theme	glycans	873:879	arg1	%					853:853	50%	851:853	50% of the identified glycans presented at levels smaller than 0.1%	851:917	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	6	52	theme	glycans	873:879	arg1	glycans					873:879	the identified glycans	858:879	the identified glycans presented at levels smaller than 0.1%	858:917	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	2	53	theme	therapeutic	228:238	arg1	proteins					240:247	the approved therapeutic proteins	215:247	the approved therapeutic proteins	215:247	Most of the approved therapeutic proteins are glycoproteins.					
33196194	3	54	theme	essential	288:296	arg1	attribute					315:323	an essential critical quality attribute	285:323	an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics	285:461	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	54	theme	essential	288:296	arg1	Glycosylation					268:280	Glycosylation	268:280	Glycosylation	268:280	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	3	54	theme	essential	288:296	arg1	CQA					326:328	CQA	326:328	CQA	326:328	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	8	55	theme	galactosylation	1005:1019	arg1	level					1021:1025	The galactosylation level	1001:1025	The galactosylation level of modified NISTmAb	1001:1045	The galactosylation level of modified NISTmAb was found to decrease by ∼10% when compared to the galactosylation level of NISTmAb.					
33196194	4	56	theme	reference	565:573	arg1	B					602:602	sample B	595:602	sample B	595:602	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	4	56	theme	reference	565:573	arg1	8671					589:592	an NISTmAb reference material (RM) 8671	554:592	an NISTmAb reference material (RM) 8671 (sample B)	554:603	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	7	57	theme	quantitative	979:990	arg1	results					992:998	the quantitative results	975:998	the quantitative results	975:998	Important glycan attributes were further derived using the quantitative results.					
33196194	2	58	theme	approved	219:226	arg1	proteins					240:247	the approved therapeutic proteins	215:247	the approved therapeutic proteins	215:247	Most of the approved therapeutic proteins are glycoproteins.					
33196194	6	59	theme	highest	753:759	arg1	abundance					761:769	the highest abundance	749:769	the highest abundance	749:769	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	7	60	theme	Important	920:928	arg1	attributes					937:946	Important glycan attributes	920:946	Important glycan attributes	920:946	Important glycan attributes were further derived using the quantitative results.					
33196194	3	61	dep	safety	380:385	arg1	the					376:378	the	376:378	the	376:378	Glycosylation is an essential critical quality attribute (CQA) due to the influence that glycoforms have on the safety, efficacy, and pharmacokinetics/pharmacodynamics (PK/PD) of biotherapeutics.					
33196194	6	62	theme	sample	816:821	arg1	A					823:823	sample A	816:823	sample A	816:823	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	10	63	theme	unglycosylated	1264:1277	arg1	peptides					1279:1286	unglycosylated peptides	1264:1286	unglycosylated peptides	1264:1286	Moreover, unglycosylated peptides were also observed at a level of 1-2%.					
33196194	4	64	theme	NISTmAb	639:645	arg1	glycans					543:549	Fc glycans	540:549	Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A)	540:656	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	4	65	theme	Fc	540:541	arg1	glycans					543:549	Fc glycans	540:549	Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A)	540:656	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	6	66	dep	sample	836:841	arg1	B.					843:844	B.	843:844	B.	843:844	The glycan structure with the highest abundance was FA2, with a relative abundance of 52% in sample A and 38% in sample B. Over 50% of the identified glycans presented at levels smaller than 0.1%.					
33196194	1	67	theme	emerging	172:179	arg1	drugs					181:185	emerging drugs	172:185	emerging drugs	172:185	Protein-based therapeutics such as mAbs have become emerging drugs in modern medicine.					
33196194	4	68	theme	β-1,4-galactosidase-treated	611:637	arg1	A					655:655	sample A	648:655	sample A	648:655	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33196194	4	68	theme	β-1,4-galactosidase-treated	611:637	arg1	NISTmAb					639:645	a β-1,4-galactosidase-treated NISTmAb	609:645	a β-1,4-galactosidase-treated NISTmAb (sample A)	609:656	Here, we applied an LC-MS/MS-based glycoproteomics approach to characterize Fc glycans of an NISTmAb reference material (RM) 8671 (sample B) and a β-1,4-galactosidase-treated NISTmAb (sample A).					
33064451	3	0	theme	spike	570:574	arg1	proteins					576:583	the SARS-CoV-2 spike proteins	555:583	the SARS-CoV-2 spike proteins	555:583	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	1	1	theme	disease	297:303	arg1	pandemic					273:280	a global pandemic	264:280	a global pandemic of coronavirus disease 2019 (COVID-19)	264:319	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	1	2	theme	syndrome	217:224	arg1	coronavirus					226:236	Severe acute respiratory syndrome coronavirus 2	192:238	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	192:251	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	1	2	theme	syndrome	217:224	arg1	SARS-CoV-2					241:250	SARS-CoV-2	241:250	SARS-CoV-2	241:250	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	4	3	theme	recombinant	741:751	arg1	ectodomain					753:762	the recombinant ectodomain	737:762	the recombinant ectodomain	737:762	The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein were characterized using this approach.					
33064451	4	3	theme	recombinant	741:751	arg1	protein					803:809	the SARS-CoV-2 spike protein	782:809	the SARS-CoV-2 spike protein	782:809	The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein were characterized using this approach.					
33064451	2	4	theme	spike	326:330	arg1	protein					332:338	The spike protein	322:338	The spike protein expressed on the surface of this virus	322:377	The spike protein expressed on the surface of this virus is highly glycosylated and plays an essential role during the process of infection.					
33064451	5	5	theme	glycans	959:965	arg1	distribution					895:906	the distribution	891:906	the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins	891:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	6	6	theme	relative	1037:1044	arg1	abundance					1046:1054	The relative abundance	1033:1054	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein	1033:1130	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	8	7	theme	SARS-CoV-2	1512:1521	arg1	proteins					1529:1536	SARS-CoV-2 viral proteins	1512:1536	SARS-CoV-2 viral proteins	1512:1536	These results provide useful information for the study of the interactions of SARS-CoV-2 viral proteins and for the development of effective vaccines and therapeutics.					
33064451	8	8	theme	proteins	1529:1536	arg1	interactions					1496:1507	the interactions	1492:1507	the interactions of SARS-CoV-2 viral proteins	1492:1536	These results provide useful information for the study of the interactions of SARS-CoV-2 viral proteins and for the development of effective vaccines and therapeutics.					
33064451	3	9	theme	collision	648:656	arg1	spectrometry					684:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	5	10	from	types	918:922	arg1	sites					987:991	the modification sites	970:991	the modification sites of the ectodomain and subunit proteins	970:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	5	10	from	types	918:922	arg1	proteins					1023:1030	the ectodomain and subunit proteins	996:1030	proteins	1023:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	4	11	theme	spike	797:801	arg1	protein					803:809	the SARS-CoV-2 spike protein	782:809	the SARS-CoV-2 spike protein	782:809	The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein were characterized using this approach.					
33064451	7	12	theme	expression	1319:1328	arg1	cells					1427:1431	Spodoptera frugiperda (SF9) insect cells	1392:1431	Spodoptera frugiperda (SF9) insect cells	1392:1431	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	7	12	theme	expression	1319:1328	arg1	cells					1382:1386	human embryonic kidney (HEK 293) cells	1349:1386	human embryonic kidney (HEK 293) cells	1349:1386	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	7	12	theme	expression	1319:1328	arg1	systems					1330:1336	different expression systems	1309:1336	different expression systems	1309:1336	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	5	13	theme	glycan	911:916	arg1	types					918:922	glycan types	911:922	glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins	911:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	8	14	theme	effective	1565:1573	arg1	vaccines					1575:1582	effective vaccines	1565:1582	effective vaccines	1565:1582	These results provide useful information for the study of the interactions of SARS-CoV-2 viral proteins and for the development of effective vaccines and therapeutics.					
33064451	5	15	theme	Significant	851:861	arg1	variations					863:872	Significant variations	851:872	Significant variations	851:872	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	1	16	theme	acute	199:203	arg1	coronavirus					226:236	Severe acute respiratory syndrome coronavirus 2	192:238	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	192:251	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	1	16	theme	acute	199:203	arg1	SARS-CoV-2					241:250	SARS-CoV-2	241:250	SARS-CoV-2	241:250	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	0	17	theme	Ions-Triggered	93:106	arg1	Spectrometry					178:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	6	18	theme	glycans	1070:1076	arg1	abundance					1046:1054	The relative abundance	1033:1054	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein	1033:1130	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	0	19	theme	Collisional	140:150	arg1	Spectrometry					178:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	2	20	theme	infection	452:460	arg1	process					441:447	the process	437:447	the process of infection	437:460	The spike protein expressed on the surface of this virus is highly glycosylated and plays an essential role during the process of infection.					
33064451	0	21	theme	Proteins	68:75	arg1	Complement					37:46	the Glycan Complement	26:46	the Glycan Complement of SARS-CoV-2 Spike Proteins	26:75	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	0	22	theme	Comprehensive	0:12	arg1	Analysis					14:21	Comprehensive Analysis	0:21	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins	0:75	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	5	23	theme	proteins	1023:1030	arg1	sites					987:991	the modification sites	970:991	the modification sites of the ectodomain and subunit proteins	970:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	5	23	theme	proteins	1023:1030	arg1	proteins					1023:1030	the ectodomain and subunit proteins	996:1030	proteins	1023:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	7	24	theme	frugiperda	1403:1412	arg1	cells					1427:1431	Spodoptera frugiperda (SF9) insect cells	1392:1431	Spodoptera frugiperda (SF9) insect cells	1392:1431	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	6	25	from	abundance	1046:1054	arg1	subunit					1088:1094	the S1 subunit	1081:1094	the S1 subunit compared to the full-length protein	1081:1130	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	8	26	theme	useful	1456:1461	arg1	information					1463:1473	useful information	1456:1473	useful information for the study of the interactions of SARS-CoV-2 viral proteins and for the development of effective vaccines and therapeutics	1456:1599	These results provide useful information for the study of the interactions of SARS-CoV-2 viral proteins and for the development of effective vaccines and therapeutics.					
33064451	7	27	theme	N-glycan	1243:1250	arg1	profiles					1252:1259	N-glycan profiles	1243:1259	N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells	1243:1431	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	2	28	theme	essential	415:423	arg1	role					425:428	an essential role	412:428	an essential role	412:428	The spike protein expressed on the surface of this virus is highly glycosylated and plays an essential role during the process of infection.					
33064451	0	29	theme	Glycan	30:35	arg1	Complement					37:46	the Glycan Complement	26:46	the Glycan Complement of SARS-CoV-2 Spike Proteins	26:75	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	6	30	theme	full-length	1112:1122	arg1	protein					1124:1130	the full-length protein	1108:1130	the full-length protein	1108:1130	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	0	31	theme	Spike	62:66	arg1	Proteins					68:75	SARS-CoV-2 Spike Proteins	51:75	SARS-CoV-2 Spike Proteins	51:75	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	3	32	theme	spectrometric	497:509	arg1	analysis					511:518	a comprehensive mass spectrometric analysis	476:518	a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins	476:583	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	6	33	theme	species	1209:1215	arg1	function					1187:1194	function	1187:1194	function	1187:1194	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	6	33	theme	species	1209:1215	arg1	structure					1173:1181	global structure	1166:1181	global structure	1166:1181	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	3	34	theme	comprehensive	478:490	arg1	analysis					511:518	a comprehensive mass spectrometric analysis	476:518	a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins	476:583	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	7	35	theme	kidney	1365:1370	arg1	cells					1382:1386	human embryonic kidney (HEK 293) cells	1349:1386	human embryonic kidney (HEK 293) cells	1349:1386	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	3	36	theme	N-glycosylation	527:541	arg1	profiles					543:550	the N-glycosylation profiles	523:550	the N-glycosylation profiles of the SARS-CoV-2 spike proteins	523:583	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	5	37	theme	specific	939:946	arg1	glycans					959:965	the specific individual glycans	935:965	glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins	911:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	7	38	theme	human	1349:1353	arg1	kidney					1365:1370	human embryonic kidney	1349:1370	human embryonic kidney (HEK 293) cells	1349:1386	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	7	38	theme	human	1349:1353	arg1	HEK					1373:1375	HEK 293	1373:1379	HEK 293	1373:1379	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	6	39	from	differences	1147:1157	arg1	function					1187:1194	function	1187:1194	function	1187:1194	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	6	39	from	differences	1147:1157	arg1	structure					1173:1181	global structure	1166:1181	global structure	1166:1181	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	3	40	theme	SARS-CoV-2	559:568	arg1	proteins					576:583	the SARS-CoV-2 spike proteins	555:583	the SARS-CoV-2 spike proteins	555:583	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	1	41	theme	coronavirus	285:295	arg1	COVID-19					311:318	COVID-19	311:318	COVID-19	311:318	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	1	41	theme	coronavirus	285:295	arg1	disease					297:303	coronavirus disease 2019	285:308	coronavirus disease 2019 (COVID-19)	285:319	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	5	42	theme	modification	974:985	arg1	sites					987:991	the modification sites	970:991	the modification sites of the ectodomain and subunit proteins	970:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	5	42	theme	modification	974:985	arg1	proteins					1023:1030	the ectodomain and subunit proteins	996:1030	proteins	1023:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	1	43	theme	respiratory	205:215	arg1	coronavirus					226:236	Severe acute respiratory syndrome coronavirus 2	192:238	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	192:251	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	1	43	theme	respiratory	205:215	arg1	SARS-CoV-2					241:250	SARS-CoV-2	241:250	SARS-CoV-2	241:250	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	4	44	theme	N-glycosylation	714:728	arg1	patterns					702:709	The patterns	698:709	The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein	698:809	The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein were characterized using this approach.					
33064451	6	45	gly	sialylated	1059:1068	arg1	glycans					1070:1076	sialylated glycans	1059:1076	sialylated glycans	1059:1076	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	3	46	theme	signature	591:599	arg1	spectrometry					684:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	5	47	theme	individual	948:957	arg1	glycans					959:965	the specific individual glycans	935:965	glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins	911:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	6	48	theme	sialylated	1059:1068	arg1	glycans					1070:1076	sialylated glycans	1059:1076	sialylated glycans	1059:1076	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	0	49	theme	EThcD	166:170	arg1	Spectrometry					178:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	3	50	theme	proteins	576:583	arg1	profiles					543:550	the N-glycosylation profiles	523:550	the N-glycosylation profiles of the SARS-CoV-2 spike proteins	523:583	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	5	51	theme	types	918:922	arg1	distribution					895:906	the distribution	891:906	the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins	891:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	3	52	theme	ions-triggered	601:614	arg1	spectrometry					684:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	8	53	theme	viral	1523:1527	arg1	proteins					1529:1536	SARS-CoV-2 viral proteins	1512:1536	SARS-CoV-2 viral proteins	1512:1536	These results provide useful information for the study of the interactions of SARS-CoV-2 viral proteins and for the development of effective vaccines and therapeutics.					
33064451	0	54	theme	Mass	173:176	arg1	Spectrometry					178:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	3	55	theme	dissociation	658:669	arg1	spectrometry					684:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	7	56	theme	spike	1280:1284	arg1	proteins					1286:1293	the recombinant spike proteins	1264:1293	the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells	1264:1431	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	3	57	theme	electron-transfer/higher-energy	616:646	arg1	spectrometry					684:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	3	58	theme	mass	679:682	arg1	spectrometry					684:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	5	59	theme	ectodomain	1000:1009	arg1	proteins					1023:1030	the ectodomain and subunit proteins	996:1030	proteins	1023:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	4	60	theme	SARS-CoV-2	786:795	arg1	protein					803:809	the SARS-CoV-2 spike protein	782:809	the SARS-CoV-2 spike protein	782:809	The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein were characterized using this approach.					
33064451	7	61	theme	different	1309:1317	arg1	cells					1427:1431	Spodoptera frugiperda (SF9) insect cells	1392:1431	Spodoptera frugiperda (SF9) insect cells	1392:1431	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	7	61	theme	different	1309:1317	arg1	cells					1382:1386	human embryonic kidney (HEK 293) cells	1349:1386	human embryonic kidney (HEK 293) cells	1349:1386	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	7	61	theme	different	1309:1317	arg1	systems					1330:1336	different expression systems	1309:1336	different expression systems	1309:1336	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	3	62	theme	EThcD	672:676	arg1	spectrometry					684:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry	591:695	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	5	63	from	glycans	959:965	arg1	sites					987:991	the modification sites	970:991	the modification sites of the ectodomain and subunit proteins	970:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	5	63	from	glycans	959:965	arg1	proteins					1023:1030	the ectodomain and subunit proteins	996:1030	proteins	1023:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	8	64	theme	vaccines	1575:1582	arg1	development					1550:1560	the development	1546:1560	the development of effective vaccines and therapeutics	1546:1599	These results provide useful information for the study of the interactions of SARS-CoV-2 viral proteins and for the development of effective vaccines and therapeutics.					
33064451	7	65	theme	insect	1420:1425	arg1	cells					1427:1431	Spodoptera frugiperda (SF9) insect cells	1392:1431	Spodoptera frugiperda (SF9) insect cells	1392:1431	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	7	66	theme	proteins	1286:1293	arg1	profiles					1252:1259	N-glycan profiles	1243:1259	N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells	1243:1431	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	0	67	theme	Signature	83:91	arg1	Spectrometry					178:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	1	68	theme	Severe	192:197	arg1	coronavirus					226:236	Severe acute respiratory syndrome coronavirus 2	192:238	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	192:251	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	1	68	theme	Severe	192:197	arg1	SARS-CoV-2					241:250	SARS-CoV-2	241:250	SARS-CoV-2	241:250	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
33064451	6	69	theme	S1	1085:1086	arg1	subunit					1088:1094	the S1 subunit	1081:1094	the S1 subunit compared to the full-length protein	1081:1130	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	8	70	theme	therapeutics	1588:1599	arg1	development					1550:1560	the development	1546:1560	the development of effective vaccines and therapeutics	1546:1599	These results provide useful information for the study of the interactions of SARS-CoV-2 viral proteins and for the development of effective vaccines and therapeutics.					
33064451	4	71	theme	S1	768:769	arg1	subunit					771:777	S1 subunit	768:777	S1 subunit	768:777	The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein were characterized using this approach.					
33064451	0	72	theme	Electron-Transfer/Higher-Energy	108:138	arg1	Spectrometry					178:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	5	73	from	sites	987:991	arg1	distribution					895:906	the distribution	891:906	the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins	891:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	3	74	gly	N-glycosylation	527:541	arg1	proteins					576:583	the SARS-CoV-2 spike proteins	555:583	the SARS-CoV-2 spike proteins	555:583	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	0	75	theme	Dissociation	152:163	arg1	Spectrometry					178:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry	83:189	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	6	76	theme	global	1166:1171	arg1	structure					1173:1181	global structure	1166:1181	global structure	1166:1181	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	5	77	from	distribution	895:906	arg1	sites					987:991	the modification sites	970:991	the modification sites of the ectodomain and subunit proteins	970:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	5	77	from	distribution	895:906	arg1	proteins					1023:1030	the ectodomain and subunit proteins	996:1030	proteins	1023:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	5	78	theme	subunit	1015:1021	arg1	proteins					1023:1030	the ectodomain and subunit proteins	996:1030	proteins	1023:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	7	79	theme	Spodoptera	1392:1401	arg1	frugiperda					1403:1412	Spodoptera frugiperda	1392:1412	Spodoptera frugiperda (SF9) insect cells	1392:1431	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	7	79	theme	Spodoptera	1392:1401	arg1	SF9					1415:1417	SF9	1415:1417	SF9	1415:1417	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	2	80	gly	glycosylated	389:400	arg1	protein					332:338	The spike protein	322:338	The spike protein expressed on the surface of this virus	322:377	The spike protein expressed on the surface of this virus is highly glycosylated and plays an essential role during the process of infection.					
33064451	4	81	theme	protein	803:809	arg1	ectodomain					753:762	the recombinant ectodomain	737:762	the recombinant ectodomain	737:762	The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein were characterized using this approach.					
33064451	4	81	theme	protein	803:809	arg1	subunit					771:777	S1 subunit	768:777	S1 subunit	768:777	The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein were characterized using this approach.					
33064451	4	81	theme	protein	803:809	arg1	protein					803:809	the SARS-CoV-2 spike protein	782:809	the SARS-CoV-2 spike protein	782:809	The patterns of N-glycosylation within the recombinant ectodomain and S1 subunit of the SARS-CoV-2 spike protein were characterized using this approach.					
33064451	5	82	located	observed	879:886	arg1	distribution					895:906	the distribution	891:906	the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins	891:1030	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	5	82	located	observed	879:886	arg2	variations					863:872	Significant variations	851:872	Significant variations	851:872	Significant variations were observed in the distribution of glycan types as well as the specific individual glycans on the modification sites of the ectodomain and subunit proteins.					
33064451	0	83	theme	Complement	37:46	arg1	Analysis					14:21	Comprehensive Analysis	0:21	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins	0:75	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	6	84	dep	structure	1173:1181	arg1	the					1162:1164	the	1162:1164	the	1162:1164	The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species.					
33064451	7	85	theme	recombinant	1268:1278	arg1	proteins					1286:1293	the recombinant spike proteins	1264:1293	the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells	1264:1431	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	0	86	theme	SARS-CoV-2	51:60	arg1	Proteins					68:75	SARS-CoV-2 Spike Proteins	51:75	SARS-CoV-2 Spike Proteins	51:75	Comprehensive Analysis of the Glycan Complement of SARS-CoV-2 Spike Proteins Using Signature Ions-Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) Mass Spectrometry.					
33064451	2	87	theme	virus	373:377	arg1	surface					357:363	the surface	353:363	the surface of this virus	353:377	The spike protein expressed on the surface of this virus is highly glycosylated and plays an essential role during the process of infection.					
33064451	7	88	theme	embryonic	1355:1363	arg1	kidney					1365:1370	human embryonic kidney	1349:1370	human embryonic kidney (HEK 293) cells	1349:1386	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	7	88	theme	embryonic	1355:1363	arg1	HEK					1373:1375	HEK 293	1373:1379	HEK 293	1373:1379	In addition, we compared N-glycan profiles of the recombinant spike proteins produced from different expression systems, including human embryonic kidney (HEK 293) cells and Spodoptera frugiperda (SF9) insect cells.					
33064451	3	89	theme	mass	492:495	arg1	analysis					511:518	a comprehensive mass spectrometric analysis	476:518	a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins	476:583	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	8	90	theme	interactions	1496:1507	arg1	study					1483:1487	the study	1479:1487	the study of the interactions of SARS-CoV-2 viral proteins	1479:1536	These results provide useful information for the study of the interactions of SARS-CoV-2 viral proteins and for the development of effective vaccines and therapeutics.					
33064451	3	91	theme	profiles	543:550	arg1	analysis					511:518	a comprehensive mass spectrometric analysis	476:518	a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins	476:583	We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry.					
33064451	1	92	theme	global	266:271	arg1	pandemic					273:280	a global pandemic	264:280	a global pandemic of coronavirus disease 2019 (COVID-19)	264:319	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a global pandemic of coronavirus disease 2019 (COVID-19).					
32576591	8	0	theme	glycan	1263:1268	arg1	masses					1270:1275	the glycan masses	1259:1275	the glycan masses before database searching	1259:1301	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	8	1	theme	cenocepacia	1147:1157	arg1	glycopeptides					1089:1101	O-linked glycopeptides	1080:1101	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans	1063:1214	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	8	1	theme	cenocepacia	1147:1157	arg1	fetus					1063:1067	fetus	1063:1067	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans	1063:1214	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	8	2	link	O-linked	1080:1087	arg1	glycopeptides					1089:1101	O-linked glycopeptides	1080:1101	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans	1063:1214	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	11	3	theme	bacterial	1891:1899	arg1	proteomes					1901:1909	bacterial proteomes	1891:1909	bacterial proteomes	1891:1909	Combined, these results show that open searching is a robust computational approach for the determination of glycan diversity within bacterial proteomes.					
32576591	0	4	theme	Glycoproteomes	79:92	arg1	Identification					36:49	Identification	36:49	Identification	36:49	Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching.					
32576591	0	4	theme	Glycoproteomes	79:92	arg1	Comparison					55:64	Comparison	55:64	Comparison	55:64	Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching.					
32576591	11	5	theme	robust	1812:1817	arg1	approach					1833:1840	a robust computational approach	1810:1840	a robust computational approach for the determination of glycan diversity within bacterial proteomes	1810:1909	Combined, these results show that open searching is a robust computational approach for the determination of glycan diversity within bacterial proteomes.					
32576591	11	5	theme	robust	1812:1817	arg1	searching					1797:1805	open searching	1792:1805	open searching	1792:1805	Combined, these results show that open searching is a robust computational approach for the determination of glycan diversity within bacterial proteomes.					
32576591	6	6	theme	open	904:907	arg1	searching					909:917	wide-tolerance (up to 2000 Da) open searching	873:917	wide-tolerance (up to 2000 Da) open searching	873:917	Here we describe the use of wide-tolerance (up to 2000 Da) open searching as a means to rapidly examine bacterial glycoproteomes.					
32576591	2	7	theme	informatic	386:395	arg1	approaches					397:406	our informatic approaches	382:406	our informatic approaches	382:406	Our ability to generate rich fragmentation of glycopeptides has dramatically improved over the last decade yet our informatic approaches still lag behind.					
32576591	4	8	theme	events	669:674	arg1	studies					624:630	high throughput studies	608:630	high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria	608:709	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32576591	0	9	theme	Glycan	111:116	arg1	Compositions					118:129	Glycan Compositions	111:129	Glycan Compositions Prior to Searching	111:148	Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching.					
32576591	11	10	theme	diversity	1874:1882	arg1	determination					1850:1862	the determination	1846:1862	the determination of glycan diversity within bacterial proteomes	1846:1909	Combined, these results show that open searching is a robust computational approach for the determination of glycan diversity within bacterial proteomes.					
32576591	8	11	theme	database	1284:1291	arg1	searching					1293:1301	database searching	1284:1301	database searching	1284:1301	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	5	12	theme	bacterial	757:765	arg1	glycosylation					767:779	bacterial glycosylation	757:779	bacterial glycosylation	757:779	As such, computational approaches to examine bacterial glycosylation and identify chemically diverse glycans are desperately needed.					
32576591	7	13	theme	subsp	1056:1060	arg1	glycopeptides					1019:1031	N-linked glycopeptides	1010:1031	N-linked glycopeptides of Campylobacter fetus subsp	1010:1060	We benchmarked this approach using N-linked glycopeptides of Campylobacter fetus subsp.					
32576591	2	14	theme	glycopeptides	317:329	arg1	fragmentation					300:312	rich fragmentation	295:312	rich fragmentation of glycopeptides	295:329	Our ability to generate rich fragmentation of glycopeptides has dramatically improved over the last decade yet our informatic approaches still lag behind.					
32576591	2	15	theme	last	366:369	arg1	decade					371:376	the last decade	362:376	the last decade	362:376	Our ability to generate rich fragmentation of glycopeptides has dramatically improved over the last decade yet our informatic approaches still lag behind.					
32576591	0	16	theme	Prior	131:135	arg1	Compositions					118:129	Glycan Compositions	111:129	Glycan Compositions Prior to Searching	111:148	Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching.					
32576591	8	17	theme	O-linked	1080:1087	arg1	glycopeptides					1089:1101	O-linked glycopeptides	1080:1101	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans	1063:1214	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	1	18	theme	indispensable	183:195	arg1	tool					197:200	an indispensable tool	180:200	an indispensable tool for the characterization of glycosylation across biological systems	180:268	Mass spectrometry has become an indispensable tool for the characterization of glycosylation across biological systems.					
32576591	7	19	theme	Campylobacter	1036:1048	arg1	subsp					1056:1060	Campylobacter fetus subsp	1036:1060	Campylobacter fetus subsp	1036:1060	We benchmarked this approach using N-linked glycopeptides of Campylobacter fetus subsp.					
32576591	3	20	theme	glycoproteomic	435:448	arg1	approaches					462:471	glycoproteomic informatics approaches	435:471	glycoproteomic informatics approaches using glycan databases	435:494	Although glycoproteomic informatics approaches using glycan databases have attracted considerable attention, database independent approaches have not.					
32576591	3	21	theme	considerable	511:522	arg1	attention					524:532	considerable attention	511:532	considerable attention	511:532	Although glycoproteomic informatics approaches using glycan databases have attracted considerable attention, database independent approaches have not.					
32576591	0	22	theme	Database	5:12	arg1	Searching					14:22	Open Database Searching	0:22	Open Database Searching	0:22	Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching.					
32576591	9	23	dep	pseudomallei	1494:1505	arg1	dolosa					1527:1532	B. dolosa	1524:1532	B. dolosa	1524:1532	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	9	23	dep	pseudomallei	1494:1505	arg1	ubonensis					1557:1565	B. ubonensis	1554:1565	B. ubonensis	1554:1565	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	9	23	dep	pseudomallei	1494:1505	arg1	pseudomultivorans					1595:1611	B. pseudomultivorans	1592:1611	B. pseudomultivorans	1592:1611	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	9	23	dep	pseudomallei	1494:1505	arg1	humptydooensis					1538:1551	B. humptydooensis	1535:1551	B. humptydooensis	1535:1551	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	9	23	dep	pseudomallei	1494:1505	arg1	multivorans					1511:1521	B. multivorans	1508:1521	B. multivorans	1508:1521	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	9	23	dep	pseudomallei	1494:1505	arg1	diffusa					1583:1589	B. diffusa	1580:1589	B. diffusa	1580:1589	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	10	24	theme	glycoforms	1702:1711	arg1	identification					1670:1683	the identification	1666:1683	the identification of low frequency glycoforms based on shared modified peptides sequences	1666:1755	Finally, we demonstrate how open searching enables the identification of low frequency glycoforms based on shared modified peptides sequences.					
32576591	7	25	theme	fetus	1050:1054	arg1	subsp					1056:1060	Campylobacter fetus subsp	1036:1060	Campylobacter fetus subsp	1036:1060	We benchmarked this approach using N-linked glycopeptides of Campylobacter fetus subsp.					
32576591	0	26	theme	Open	0:3	arg1	Searching					14:22	Open Database Searching	0:22	Open Database Searching	0:22	Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching.					
32576591	5	27	theme	computational	721:733	arg1	such					715:718	such	715:718	such	715:718	As such, computational approaches to examine bacterial glycosylation and identify chemically diverse glycans are desperately needed.					
32576591	5	27	theme	computational	721:733	arg1	approaches					735:744	computational approaches	721:744	computational approaches to examine bacterial glycosylation and identify chemically diverse glycans	721:819	As such, computational approaches to examine bacterial glycosylation and identify chemically diverse glycans are desperately needed.					
32576591	9	28	theme	wide	1344:1347	arg1	searching					1359:1367	wide tolerance searching	1344:1367	wide tolerance searching	1344:1367	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	7	29	link	N-linked	1010:1017	arg1	glycopeptides					1019:1031	N-linked glycopeptides	1010:1031	N-linked glycopeptides of Campylobacter fetus subsp	1010:1060	We benchmarked this approach using N-linked glycopeptides of Campylobacter fetus subsp.					
32576591	5	30	dep	approaches	735:744	arg1	identify					785:792	identify	785:792	identify chemically diverse glycans	785:819	As such, computational approaches to examine bacterial glycosylation and identify chemically diverse glycans are desperately needed.					
32576591	5	30	dep	approaches	735:744	arg1	examine					749:755	examine	749:755	examine bacterial glycosylation	749:779	As such, computational approaches to examine bacterial glycosylation and identify chemically diverse glycans are desperately needed.					
32576591	4	31	theme	unusual	635:641	arg1	events					669:674	unusual or atypical glycosylation events	635:674	unusual or atypical glycosylation events such as those observed in bacteria	635:709	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32576591	4	31	theme	unusual	635:641	arg1	those					684:688	those	684:688	those	684:688	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32576591	5	32	theme	diverse	805:811	arg1	glycans					813:819	chemically diverse glycans	794:819	chemically diverse glycans	794:819	As such, computational approaches to examine bacterial glycosylation and identify chemically diverse glycans are desperately needed.					
32576591	8	33	theme	baumannii	1120:1128	arg1	glycopeptides					1089:1101	O-linked glycopeptides	1080:1101	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans	1063:1214	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	8	33	theme	baumannii	1120:1128	arg1	fetus					1063:1067	fetus	1063:1067	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans	1063:1214	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	6	34	dep	2000	895:898	arg1	to					892:893	to	892:893	to	892:893	Here we describe the use of wide-tolerance (up to 2000 Da) open searching as a means to rapidly examine bacterial glycoproteomes.					
32576591	3	35	theme	database	535:542	arg1	approaches					556:565	database independent approaches	535:565	database independent approaches	535:565	Although glycoproteomic informatics approaches using glycan databases have attracted considerable attention, database independent approaches have not.					
32576591	8	36	gly	glycopeptides	1089:1101	arg2	glycopeptides					1089:1101	O-linked glycopeptides	1080:1101	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans	1063:1214	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	4	37	theme	glycosylation	655:667	arg1	events					669:674	unusual or atypical glycosylation events	635:674	unusual or atypical glycosylation events such as those observed in bacteria	635:709	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32576591	4	37	theme	glycosylation	655:667	arg1	those					684:688	those	684:688	those	684:688	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32576591	6	38	theme	bacterial	949:957	arg1	glycoproteomes					959:972	bacterial glycoproteomes	949:972	bacterial glycoproteomes	949:972	Here we describe the use of wide-tolerance (up to 2000 Da) open searching as a means to rapidly examine bacterial glycoproteomes.					
32576591	10	39	theme	open	1643:1646	arg1	searching					1648:1656	open searching	1643:1656	open searching	1643:1656	Finally, we demonstrate how open searching enables the identification of low frequency glycoforms based on shared modified peptides sequences.					
32576591	6	40	dep	open	904:907	arg1	Da					900:901	up to 2000 Da	889:901	up to 2000 Da	889:901	Here we describe the use of wide-tolerance (up to 2000 Da) open searching as a means to rapidly examine bacterial glycoproteomes.					
32576591	3	41	theme	independent	544:554	arg1	approaches					556:565	database independent approaches	535:565	database independent approaches	535:565	Although glycoproteomic informatics approaches using glycan databases have attracted considerable attention, database independent approaches have not.					
32576591	10	42	theme	peptides	1738:1745	arg1	sequences					1747:1755	shared modified peptides sequences	1722:1755	shared modified peptides sequences	1722:1755	Finally, we demonstrate how open searching enables the identification of low frequency glycoforms based on shared modified peptides sequences.					
32576591	4	43	theme	atypical	646:653	arg1	events					669:674	unusual or atypical glycosylation events	635:674	unusual or atypical glycosylation events such as those observed in bacteria	635:709	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32576591	4	43	theme	atypical	646:653	arg1	those					684:688	those	684:688	those	684:688	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32576591	10	44	theme	low	1688:1690	arg1	glycoforms					1702:1711	low frequency glycoforms	1688:1711	low frequency glycoforms based on shared modified peptides sequences	1688:1755	Finally, we demonstrate how open searching enables the identification of low frequency glycoforms based on shared modified peptides sequences.					
32576591	4	45	located	observed	690:697	arg2	those					684:688	those	684:688	those	684:688	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32576591	4	45	located	observed	690:697	arg1	bacteria					702:709	bacteria	702:709	bacteria	702:709	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32576591	11	46	theme	computational	1819:1831	arg1	approach					1833:1840	a robust computational approach	1810:1840	a robust computational approach for the determination of glycan diversity within bacterial proteomes	1810:1909	Combined, these results show that open searching is a robust computational approach for the determination of glycan diversity within bacterial proteomes.					
32576591	11	46	theme	computational	1819:1831	arg1	searching					1797:1805	open searching	1792:1805	open searching	1792:1805	Combined, these results show that open searching is a robust computational approach for the determination of glycan diversity within bacterial proteomes.					
32576591	1	47	theme	glycosylation	230:242	arg1	characterization					210:225	the characterization	206:225	the characterization of glycosylation across biological systems	206:268	Mass spectrometry has become an indispensable tool for the characterization of glycosylation across biological systems.					
32576591	3	48	theme	glycan	479:484	arg1	databases					486:494	glycan databases	479:494	glycan databases	479:494	Although glycoproteomic informatics approaches using glycan databases have attracted considerable attention, database independent approaches have not.					
32576591	4	49	theme	throughput	613:622	arg1	studies					624:630	high throughput studies	608:630	high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria	608:709	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32576591	10	50	theme	modified	1729:1736	arg1	sequences					1747:1755	shared modified peptides sequences	1722:1755	shared modified peptides sequences	1722:1755	Finally, we demonstrate how open searching enables the identification of low frequency glycoforms based on shared modified peptides sequences.					
32576591	11	51	theme	open	1792:1795	arg1	approach					1833:1840	a robust computational approach	1810:1840	a robust computational approach for the determination of glycan diversity within bacterial proteomes	1810:1909	Combined, these results show that open searching is a robust computational approach for the determination of glycan diversity within bacterial proteomes.					
32576591	11	51	theme	open	1792:1795	arg1	searching					1797:1805	open searching	1792:1805	open searching	1792:1805	Combined, these results show that open searching is a robust computational approach for the determination of glycan diversity within bacterial proteomes.					
32576591	10	52	theme	frequency	1692:1700	arg1	glycoforms					1702:1711	low frequency glycoforms	1688:1711	low frequency glycoforms based on shared modified peptides sequences	1688:1755	Finally, we demonstrate how open searching enables the identification of low frequency glycoforms based on shared modified peptides sequences.					
32576591	3	53	theme	informatics	450:460	arg1	approaches					462:471	glycoproteomic informatics approaches	435:471	glycoproteomic informatics approaches using glycan databases	435:494	Although glycoproteomic informatics approaches using glycan databases have attracted considerable attention, database independent approaches have not.					
32576591	2	54	gly	glycopeptides	317:329	arg2	glycopeptides					317:329	glycopeptides	317:329	glycopeptides	317:329	Our ability to generate rich fragmentation of glycopeptides has dramatically improved over the last decade yet our informatic approaches still lag behind.					
32576591	9	55	used	used	1376:1379	arg2	searching					1359:1367	wide tolerance searching	1344:1367	wide tolerance searching	1344:1367	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	9	56	theme	Burkholderia	1469:1480	arg1	species					1482:1488	eight Burkholderia species	1463:1488	eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans)	1463:1612	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	0	57	dep	Identification	36:49	arg1	the					32:34	the	32:34	the	32:34	Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching.					
32576591	8	58	theme	glycans	1208:1214	arg1	range					1199:1203	a range	1197:1203	a range of glycans	1197:1214	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	9	59	theme	bacterial	1410:1418	arg1	species					1420:1426	bacterial species	1410:1426	bacterial species	1410:1426	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	9	60	theme	tolerance	1349:1357	arg1	searching					1359:1367	wide tolerance searching	1344:1367	wide tolerance searching	1344:1367	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	10	61	gly	glycoforms	1702:1711	arg1	frequency					1692:1700	low frequency glycoforms	1688:1711	low frequency glycoforms based on shared modified peptides sequences	1688:1755	Finally, we demonstrate how open searching enables the identification of low frequency glycoforms based on shared modified peptides sequences.					
32576591	2	62	theme	rich	295:298	arg1	fragmentation					300:312	rich fragmentation	295:312	rich fragmentation of glycopeptides	295:329	Our ability to generate rich fragmentation of glycopeptides has dramatically improved over the last decade yet our informatic approaches still lag behind.					
32576591	9	63	theme	species	1482:1488	arg1	glycoproteomes					1445:1458	the glycoproteomes	1441:1458	the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans)	1441:1612	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	7	64	theme	N-linked	1010:1017	arg1	glycopeptides					1019:1031	N-linked glycopeptides	1010:1031	N-linked glycopeptides of Campylobacter fetus subsp	1010:1060	We benchmarked this approach using N-linked glycopeptides of Campylobacter fetus subsp.					
32576591	1	65	theme	biological	251:260	arg1	systems					262:268	biological systems	251:268	biological systems	251:268	Mass spectrometry has become an indispensable tool for the characterization of glycosylation across biological systems.					
32576591	9	66	theme	glycan	1392:1397	arg1	use					1399:1401	glycan use	1392:1401	glycan use	1392:1401	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	1	67	theme	Mass	151:154	arg1	spectrometry					156:167	Mass spectrometry	151:167	Mass spectrometry	151:167	Mass spectrometry has become an indispensable tool for the characterization of glycosylation across biological systems.					
32576591	11	68	theme	glycan	1867:1872	arg1	diversity					1874:1882	glycan diversity	1867:1882	glycan diversity within bacterial proteomes	1867:1909	Combined, these results show that open searching is a robust computational approach for the determination of glycan diversity within bacterial proteomes.					
32576591	0	69	theme	Bacterial	69:77	arg1	Glycoproteomes					79:92	Bacterial Glycoproteomes	69:92	Bacterial Glycoproteomes	69:92	Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching.					
32576591	6	70	theme	searching	909:917	arg1	use					866:868	the use	862:868	the use of wide-tolerance (up to 2000 Da) open searching as a means to rapidly examine bacterial glycoproteomes	862:972	Here we describe the use of wide-tolerance (up to 2000 Da) open searching as a means to rapidly examine bacterial glycoproteomes.					
32576591	2	71	theme	lag	414:416	arg1	behind					418:423	lag behind	414:423	lag behind	414:423	Our ability to generate rich fragmentation of glycopeptides has dramatically improved over the last decade yet our informatic approaches still lag behind.					
32576591	8	72	gly	glycopeptides	1169:1181	arg2	glycopeptides					1169:1181	glycopeptides	1169:1181	glycopeptides modified with a range of glycans	1169:1214	fetus as well as O-linked glycopeptides of Acinetobacter baumannii and Burkholderia cenocepacia revealing glycopeptides modified with a range of glycans can be readily identified without defining the glycan masses before database searching.					
32576591	10	73	theme	shared	1722:1727	arg1	sequences					1747:1755	shared modified peptides sequences	1722:1755	shared modified peptides sequences	1722:1755	Finally, we demonstrate how open searching enables the identification of low frequency glycoforms based on shared modified peptides sequences.					
32576591	9	74	dep	species	1482:1488	arg1	pseudomallei					1494:1505	B. pseudomallei	1491:1505	B. pseudomallei	1491:1505	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	9	74	dep	species	1482:1488	arg1	anthina					1571:1577	B. anthina	1568:1577	B. anthina	1568:1577	Using this approach, we demonstrate how wide tolerance searching can be used to compare glycan use across bacterial species by examining the glycoproteomes of eight Burkholderia species (B. pseudomallei; B. multivorans; B. dolosa; B. humptydooensis; B. ubonensis, B. anthina; B. diffusa; B. pseudomultivorans).					
32576591	7	75	gly	glycopeptides	1019:1031	arg2	glycopeptides					1019:1031	N-linked glycopeptides	1010:1031	N-linked glycopeptides of Campylobacter fetus subsp	1010:1060	We benchmarked this approach using N-linked glycopeptides of Campylobacter fetus subsp.					
32576591	7	75	gly	glycopeptides	1019:1031	arg1	subsp					1056:1060	Campylobacter fetus subsp	1036:1060	Campylobacter fetus subsp	1036:1060	We benchmarked this approach using N-linked glycopeptides of Campylobacter fetus subsp.					
32576591	4	76	theme	high	608:611	arg1	studies					624:630	high throughput studies	608:630	high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria	608:709	This has significantly limited high throughput studies of unusual or atypical glycosylation events such as those observed in bacteria.					
32841605	3	0	theme	natural	554:560	arg1	variants					562:569	natural variants	554:569	natural variants	554:569	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	6	1	theme	therapeutic	1153:1163	arg1	strategies					1165:1174	therapeutic strategies	1153:1174	therapeutic strategies	1153:1174	Taken together, these data can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32841605	3	2	theme	variants	562:569	arg1	analyses					542:549	bioinformatics analyses	527:549	bioinformatics analyses of natural variants	527:569	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	5	3	from	influence	986:994	arg1	glycosylation					1033:1045	ACE2 receptor glycosylation	1019:1045	ACE2 receptor glycosylation	1019:1045	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	5	3	from	influence	986:994	arg1	glycosylation					956:968	Spike glycosylation	950:968	Spike glycosylation	950:968	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	2	4	used	utilized	293:300	arg2	We					290:291	We	290:291	We	290:291	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	1	5	theme	host	273:276	arg1	entry					283:287	host cell entry	273:287	host cell entry	273:287	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	5	6	theme	ACE2	1019:1022	arg1	glycosylation					1033:1045	ACE2 receptor glycosylation	1019:1045	ACE2 receptor glycosylation	1019:1045	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	3	7	gly	glycoprotein	676:687	arg1	glycoprotein					676:687	each glycoprotein	671:687	each glycoprotein	671:687	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	1	8	gly	glycoprotein	245:256	arg1	glycoprotein					245:256	the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein	180:256	the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein	180:256	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	4	9	theme	Spike-ACE2	841:850	arg1	interactions					852:863	Spike-ACE2 interactions	841:863	Spike-ACE2 interactions	841:863	Our results highlight roles for glycans in sterically masking polypeptide epitopes and directly modulating Spike-ACE2 interactions.					
32841605	1	10	theme	cell	278:281	arg1	entry					283:287	host cell entry	273:287	host cell entry	273:287	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	5	11	theme	divergence	936:945	arg1	impact					906:911	the impact	902:911	the impact of viral evolution and divergence on Spike glycosylation	902:968	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	5	11	theme	divergence	936:945	arg1	influence					986:994	the influence	982:994	the influence of natural variants on ACE2 receptor glycosylation	982:1045	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	2	12	theme	soluble	462:468	arg1	version					470:476	a soluble version	460:476	a soluble version of human ACE2	460:490	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	2	13	theme	mimetic	434:440	arg1	immunogen					442:450	a recombinant trimer Spike mimetic immunogen	407:450	a recombinant trimer Spike mimetic immunogen	407:450	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	5	14	theme	Spike	950:954	arg1	glycosylation					956:968	Spike glycosylation	950:968	Spike glycosylation	950:968	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	2	15	theme	ACE2	487:490	arg1	version					470:476	a soluble version	460:476	a soluble version of human ACE2	460:490	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	1	16	theme	cell	184:187	arg1	enzyme					229:234	the cell surface receptor angiotensin converting enzyme 2	180:236	the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein	180:256	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	1	16	theme	cell	184:187	arg1	ACE2					239:242	ACE2	239:242	ACE2	239:242	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	5	17	from	impact	906:911	arg1	glycosylation					1033:1045	ACE2 receptor glycosylation	1019:1045	ACE2 receptor glycosylation	1019:1045	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	5	17	from	impact	906:911	arg1	glycosylation					956:968	Spike glycosylation	950:968	Spike glycosylation	950:968	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	6	18	theme	viral	1187:1191	arg1	infection					1193:1201	viral infection	1187:1201	viral infection	1187:1201	Taken together, these data can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32841605	1	19	theme	SARS-CoV-2	90:99	arg1	betacoronavirus					101:115	The SARS-CoV-2 betacoronavirus	86:115	The SARS-CoV-2 betacoronavirus	86:115	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	1	20	theme	surface	189:195	arg1	enzyme					229:234	the cell surface receptor angiotensin converting enzyme 2	180:236	the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein	180:256	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	1	20	theme	surface	189:195	arg1	ACE2					239:242	ACE2	239:242	ACE2	239:242	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	0	21	theme	Virus-Receptor	0:13	arg1	Interactions					15:26	Virus-Receptor Interactions	0:26	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor	0:83	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32841605	3	22	theme	glycoproteins	611:623	arg1	structures					592:601	3D structures	589:601	3D structures of both glycoproteins	589:623	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	5	23	theme	receptor	1024:1031	arg1	glycosylation					1033:1045	ACE2 receptor glycosylation	1019:1045	ACE2 receptor glycosylation	1019:1045	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	1	24	theme	receptor	197:204	arg1	enzyme					229:234	the cell surface receptor angiotensin converting enzyme 2	180:236	the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein	180:256	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	1	24	theme	receptor	197:204	arg1	ACE2					239:242	ACE2	239:242	ACE2	239:242	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	0	25	theme	Glycosylated	31:42	arg1	Spike					55:59	Glycosylated SARS-CoV-2 Spike	31:59	Glycosylated SARS-CoV-2 Spike	31:59	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32841605	1	26	gly	glycosylated	133:144	arg1	protein					161:167	its highly glycosylated trimeric Spike protein	122:167	its highly glycosylated trimeric Spike protein	122:167	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	1	27	theme	angiotensin	206:216	arg1	enzyme					229:234	the cell surface receptor angiotensin converting enzyme 2	180:236	the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein	180:256	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	1	27	theme	angiotensin	206:216	arg1	ACE2					239:242	ACE2	239:242	ACE2	239:242	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	5	28	theme	viral	916:920	arg1	evolution					922:930	viral evolution	916:930	viral evolution	916:930	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	5	29	theme	variants	1007:1014	arg1	impact					906:911	the impact	902:911	the impact of viral evolution and divergence on Spike glycosylation	902:968	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	5	29	theme	variants	1007:1014	arg1	influence					986:994	the influence	982:994	the influence of natural variants on ACE2 receptor glycosylation	982:1045	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	2	30	theme	glycomics-informed	302:319	arg1	glycoproteomics					321:335	glycomics-informed glycoproteomics	302:335	glycomics-informed glycoproteomics	302:335	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	3	31	dep	generate	628:635	arg1	both					689:692	both	689:692	both	689:692	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	3	31	dep	generate	628:635	arg1	interacting					704:714	interacting	704:714	interacting with one another	704:731	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	1	32	theme	converting	218:227	arg1	enzyme					229:234	the cell surface receptor angiotensin converting enzyme 2	180:236	the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein	180:256	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	1	32	theme	converting	218:227	arg1	ACE2					239:242	ACE2	239:242	ACE2	239:242	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	0	33	theme	Spike	55:59	arg1	Interactions					15:26	Virus-Receptor Interactions	0:26	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor	0:83	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32841605	5	34	theme	natural	999:1005	arg1	variants					1007:1014	natural variants	999:1014	natural variants	999:1014	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	3	35	theme	3D	589:590	arg1	structures					592:601	3D structures	589:601	3D structures of both glycoproteins	589:623	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	2	36	theme	Spike	428:432	arg1	immunogen					442:450	a recombinant trimer Spike mimetic immunogen	407:450	a recombinant trimer Spike mimetic immunogen	407:450	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	1	37	theme	enzyme	229:234	arg1	glycoprotein					245:256	the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein	180:256	the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein	180:256	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	0	38	theme	SARS-CoV-2	44:53	arg1	Spike					55:59	Glycosylated SARS-CoV-2 Spike	31:59	Glycosylated SARS-CoV-2 Spike	31:59	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32841605	2	39	theme	human	481:485	arg1	ACE2					487:490	human ACE2	481:490	human ACE2	481:490	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	0	40	theme	Human	65:69	arg1	Receptor					76:83	Human ACE2 Receptor	65:83	Human ACE2 Receptor	65:83	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32841605	3	41	theme	molecular	637:645	arg1	simulations					656:666	molecular dynamics simulations	637:666	molecular dynamics simulations of each glycoprotein	637:687	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	2	42	theme	glycosylation	389:401	arg1	microheterogeneity					367:384	site-specific microheterogeneity	353:384	site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2	353:490	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	2	43	theme	site-specific	353:365	arg1	microheterogeneity					367:384	site-specific microheterogeneity	353:384	site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2	353:490	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	3	44	dep	both	689:692	arg1	alone					694:698	alone	694:698	alone	694:698	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	3	45	theme	dynamics	647:654	arg1	simulations					656:666	molecular dynamics simulations	637:666	molecular dynamics simulations of each glycoprotein	637:687	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	1	46	theme	glycosylated	133:144	arg1	protein					161:167	its highly glycosylated trimeric Spike protein	122:167	its highly glycosylated trimeric Spike protein	122:167	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	1	47	theme	trimeric	146:153	arg1	protein					161:167	its highly glycosylated trimeric Spike protein	122:167	its highly glycosylated trimeric Spike protein	122:167	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	6	48	theme	antibody	1118:1125	arg1	neutralization					1127:1140	antibody neutralization	1118:1140	antibody neutralization	1118:1140	Taken together, these data can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32841605	2	49	gly	microheterogeneity	367:384	arg1	glycosylation					389:401	glycosylation	389:401	glycosylation	389:401	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	4	50	theme	polypeptide	796:806	arg1	epitopes					808:815	polypeptide epitopes	796:815	polypeptide epitopes	796:815	Our results highlight roles for glycans in sterically masking polypeptide epitopes and directly modulating Spike-ACE2 interactions.					
32841605	1	51	theme	Spike	155:159	arg1	protein					161:167	its highly glycosylated trimeric Spike protein	122:167	its highly glycosylated trimeric Spike protein	122:167	The SARS-CoV-2 betacoronavirus uses its highly glycosylated trimeric Spike protein to bind to the cell surface receptor angiotensin converting enzyme 2 (ACE2) glycoprotein and facilitate host cell entry.					
32841605	3	52	gly	glycoproteins	611:623	arg1	glycoproteins					611:623	both glycoproteins	606:623	both glycoproteins	606:623	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	0	53	theme	Receptor	76:83	arg1	Interactions					15:26	Virus-Receptor Interactions	0:26	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor	0:83	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32841605	5	54	theme	evolution	922:930	arg1	impact					906:911	the impact	902:911	the impact of viral evolution and divergence on Spike glycosylation	902:968	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	5	54	theme	evolution	922:930	arg1	influence					986:994	the influence	982:994	the influence of natural variants on ACE2 receptor glycosylation	982:1045	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation.					
32841605	2	55	theme	trimer	421:426	arg1	immunogen					442:450	a recombinant trimer Spike mimetic immunogen	407:450	a recombinant trimer Spike mimetic immunogen	407:450	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	3	56	theme	bioinformatics	527:540	arg1	analyses					542:549	bioinformatics analyses	527:549	bioinformatics analyses of natural variants	527:569	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	0	57	theme	ACE2	71:74	arg1	Receptor					76:83	Human ACE2 Receptor	65:83	Human ACE2 Receptor	65:83	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32841605	3	58	theme	glycoprotein	676:687	arg1	simulations					656:666	molecular dynamics simulations	637:666	molecular dynamics simulations of each glycoprotein	637:687	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32841605	2	59	theme	recombinant	409:419	arg1	trimer					421:426	a recombinant trimer	407:426	a recombinant trimer Spike mimetic immunogen	407:450	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32841605	3	60	dep	one	721:723	arg1	another					725:731	another	725:731	another	725:731	We combined this information with bioinformatics analyses of natural variants and with existing 3D structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein both alone and interacting with one another.					
32086860	0	0	theme	One-Pot	96:102	arg1	Strategy					118:125	Orthogonal One-Pot Glycosylation Strategy	85:125	Orthogonal One-Pot Glycosylation Strategy	85:125	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides: Orthogonal One-Pot Glycosylation Strategy.					
32086860	4	1	theme	glycosylation	995:1007	arg1	strategy					1009:1016	This orthogonal one-pot glycosylation strategy	971:1016	This orthogonal one-pot glycosylation strategy	971:1016	This orthogonal one-pot glycosylation strategy can streamline the chemical synthesis of long, branched, and complicated carbohydrate chains.					
32086860	3	2	theme	final	731:735	arg1	O5					726:727	first O3', second O5'	708:728	O5	726:727	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	3	theme	reagent	840:846	arg1	control					848:854	reagent control	840:854	reagent control	840:854	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	0	4	theme	Orthogonal	85:94	arg1	Strategy					118:125	Orthogonal One-Pot Glycosylation Strategy	85:125	Orthogonal One-Pot Glycosylation Strategy	85:125	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides: Orthogonal One-Pot Glycosylation Strategy.					
32086860	1	5	theme	complex	165:171	arg1	sequences					186:194	long, branched, and complex carbohydrate sequences	145:194	long, branched, and complex carbohydrate sequences	145:194	The synthesis of long, branched, and complex carbohydrate sequences remains a challenging task in chemical synthesis.					
32086860	3	6	theme	 several	483:490	arg1	reactions					514:522	 several one-pot glycosylation reactions	483:522	 several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation	483:596	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	7	theme	successful	663:672	arg1	sequences					697:705	 the successful and efficient assembly sequences	658:705	2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif	656:789	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	8	theme	Yu	581:582	arg1	basis					531:535	the basis	527:535	the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation	527:596	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	9	dep	PTFAI	570:574	arg1	glycosylation					584:596	glycosylation	584:596	glycosylation	584:596	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	10	theme	1,2-cis-glucosylation	815:835	arg1	synthesis					625:633	the chemical synthesis	612:633	the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide	612:968	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	0	11	theme	Glycosylation	104:116	arg1	Strategy					118:125	Orthogonal One-Pot Glycosylation Strategy	85:125	Orthogonal One-Pot Glycosylation Strategy	85:125	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides: Orthogonal One-Pot Glycosylation Strategy.					
32086860	4	12	theme	chemical	1037:1044	arg1	synthesis					1046:1054	the chemical synthesis	1033:1054	the chemical synthesis of long, branched, and complicated carbohydrate chains	1033:1109	This orthogonal one-pot glycosylation strategy can streamline the chemical synthesis of long, branched, and complicated carbohydrate chains.					
32086860	3	13	dep	streamline	601:610	arg1	1					481:481	1	481:481	1	481:481	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	14	theme	efficient	678:686	arg1	sequences					697:705	 the successful and efficient assembly sequences	658:705	2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif	656:789	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	15	theme	reaction	904:911	arg1	synthesis					625:633	the chemical synthesis	612:633	the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide	612:968	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	2	16	dep	nona-decasaccharide	311:329	arg1	a					309:309	a	309:309	a	309:309	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	0	17	dep	Strategy	118:125	arg1	Synthesis					8:16	Modular Synthesis	0:16	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides	0:82	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides: Orthogonal One-Pot Glycosylation Strategy.					
32086860	3	18	theme	strategy	462:469	arg1	features					471:478	The synthetic strategy features	448:478	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.	448:969	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	1	19	theme	carbohydrate	173:184	arg1	sequences					186:194	long, branched, and complex carbohydrate sequences	145:194	long, branched, and complex carbohydrate sequences	145:194	The synthesis of long, branched, and complex carbohydrate sequences remains a challenging task in chemical synthesis.					
32086860	3	20	dep	1,2-cis-glucosylation	815:835	arg1	3					792:792	3	792:792	3	792:792	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	21	from	reactions	514:522	arg1	basis					531:535	the basis	527:535	the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation	527:596	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	2	22	theme	modular	280:286	arg1	synthesis					296:304	an efficient and modular one-pot synthesis	263:304	an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity	263:445	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	3	23	theme	one-pot	887:893	arg1	reaction					904:911	 the convergent [6+6+7] one-pot coupling reaction	863:911	4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide	861:968	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	1	24	theme	sequences	186:194	arg1	synthesis					132:140	The synthesis	128:140	The synthesis of long, branched, and complex carbohydrate sequences	128:194	The synthesis of long, branched, and complex carbohydrate sequences remains a challenging task in chemical synthesis.					
32086860	2	25	contain	have	397:400	arg1	nona-decasaccharide					311:329	nona-decasaccharide	311:329	nona-decasaccharide	311:329	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	2	25	contain	have	397:400	arg1	sequences					343:351	shorter sequences	335:351	shorter sequences from Psidium guajava polysaccharides	335:388	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	2	25	contain	have	397:400	arg2	activity					438:445	the potent α-glucosidase inhibitory activity	402:445	the potent α-glucosidase inhibitory activity	402:445	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	2	26	from	polysaccharides	374:388	arg1	synthesis					296:304	an efficient and modular one-pot synthesis	263:304	an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity	263:445	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	2	26	from	polysaccharides	374:388	arg1	nona-decasaccharide					311:329	nona-decasaccharide	311:329	nona-decasaccharide	311:329	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	2	26	from	polysaccharides	374:388	arg1	sequences					343:351	shorter sequences	335:351	shorter sequences from Psidium guajava polysaccharides	335:388	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	2	27	theme	shorter	335:341	arg1	sequences					343:351	shorter sequences	335:351	shorter sequences from Psidium guajava polysaccharides	335:388	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	3	28	theme	coupling	895:902	arg1	reaction					904:911	 the convergent [6+6+7] one-pot coupling reaction	863:911	4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide	861:968	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	2	29	theme	potent	406:411	arg1	activity					438:445	the potent α-glucosidase inhibitory activity	402:445	the potent α-glucosidase inhibitory activity	402:445	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	0	30	theme	Modular	0:6	arg1	Synthesis					8:16	Modular Synthesis	0:16	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides	0:82	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides: Orthogonal One-Pot Glycosylation Strategy.					
32086860	3	31	theme	chemical	616:623	arg1	synthesis					625:633	the chemical synthesis	612:633	the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide	612:968	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	4	32	theme	chains	1104:1109	arg1	synthesis					1046:1054	the chemical synthesis	1033:1054	the chemical synthesis of long, branched, and complicated carbohydrate chains	1033:1109	This orthogonal one-pot glycosylation strategy can streamline the chemical synthesis of long, branched, and complicated carbohydrate chains.					
32086860	0	33	theme	Nona-Decasaccharide	21:39	arg1	Motif					41:45	Nona-Decasaccharide Motif	21:45	Nona-Decasaccharide Motif	21:45	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides: Orthogonal One-Pot Glycosylation Strategy.					
32086860	3	34	theme	challenging	753:763	arg1	motif					785:789	the challenging 2,3,5-branched Araf motif	749:789	the challenging 2,3,5-branched Araf motif	749:789	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	2	35	theme	nona-decasaccharide	311:329	arg1	synthesis					296:304	an efficient and modular one-pot synthesis	263:304	an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity	263:445	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	1	36	theme	challenging	206:216	arg1	task					218:221	a challenging task	204:221	a challenging task in chemical synthesis	204:243	The synthesis of long, branched, and complex carbohydrate sequences remains a challenging task in chemical synthesis.					
32086860	2	37	theme	one-pot	288:294	arg1	synthesis					296:304	an efficient and modular one-pot synthesis	263:304	an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity	263:445	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	3	38	theme	assembly	688:695	arg1	sequences					697:705	 the successful and efficient assembly sequences	658:705	2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif	656:789	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	39	theme	final	921:925	arg1	assembly					927:934	the final assembly	917:934	the final assembly of the target nona-decasaccharide	917:968	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	40	theme	glycosylation	500:512	arg1	reactions					514:522	 several one-pot glycosylation reactions	483:522	 several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation	483:596	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	41	theme	sequences	697:705	arg1	synthesis					625:633	the chemical synthesis	612:633	the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide	612:968	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	42	theme	oligosaccharides	638:653	arg1	synthesis					625:633	the chemical synthesis	612:633	the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide	612:968	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	2	43	from	synthesis	296:304	arg1	polysaccharides					374:388	Psidium guajava polysaccharides	358:388	Psidium guajava polysaccharides	358:388	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	0	44	theme	Motif	41:45	arg1	Synthesis					8:16	Modular Synthesis	0:16	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides	0:82	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides: Orthogonal One-Pot Glycosylation Strategy.					
32086860	4	45	theme	one-pot	987:993	arg1	strategy					1009:1016	This orthogonal one-pot glycosylation strategy	971:1016	This orthogonal one-pot glycosylation strategy	971:1016	This orthogonal one-pot glycosylation strategy can streamline the chemical synthesis of long, branched, and complicated carbohydrate chains.					
32086860	3	46	theme	synthetic	452:460	arg1	features					471:478	The synthetic strategy features	448:478	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.	448:969	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	47	theme	2,3,5-branched	765:778	arg1	motif					785:789	the challenging 2,3,5-branched Araf motif	749:789	the challenging 2,3,5-branched Araf motif	749:789	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	4	48	theme	long	1059:1062	arg1	chains					1104:1109	long, branched, and complicated carbohydrate chains	1059:1109	long, branched, and complicated carbohydrate chains	1059:1109	This orthogonal one-pot glycosylation strategy can streamline the chemical synthesis of long, branched, and complicated carbohydrate chains.					
32086860	3	49	theme	nona-decasaccharide	950:968	arg1	assembly					927:934	the final assembly	917:934	the final assembly of the target nona-decasaccharide	917:968	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	1	50	theme	chemical	226:233	arg1	synthesis					235:243	chemical synthesis	226:243	chemical synthesis	226:243	The synthesis of long, branched, and complex carbohydrate sequences remains a challenging task in chemical synthesis.					
32086860	0	51	theme	guajava	60:66	arg1	Polysaccharides					68:82	Psidium guajava Polysaccharides	52:82	Psidium guajava Polysaccharides	52:82	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides: Orthogonal One-Pot Glycosylation Strategy.					
32086860	3	52	dep	final	731:735	arg1	O2					737:738	O2	737:738	O2	737:738	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	0	53	from	Polysaccharides	68:82	arg1	Synthesis					8:16	Modular Synthesis	0:16	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides	0:82	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides: Orthogonal One-Pot Glycosylation Strategy.					
32086860	2	54	theme	guajava	366:372	arg1	polysaccharides					374:388	Psidium guajava polysaccharides	358:388	Psidium guajava polysaccharides	358:388	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	0	55	theme	Psidium	52:58	arg1	Polysaccharides					68:82	Psidium guajava Polysaccharides	52:82	Psidium guajava Polysaccharides	52:82	Modular Synthesis of Nona-Decasaccharide Motif from Psidium guajava Polysaccharides: Orthogonal One-Pot Glycosylation Strategy.					
32086860	2	56	theme	inhibitory	427:436	arg1	activity					438:445	the potent α-glucosidase inhibitory activity	402:445	the potent α-glucosidase inhibitory activity	402:445	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	3	57	theme	O3	714:715	arg1	O5					726:727	first O3', second O5'	708:728	O5	726:727	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	58	dep	reaction	904:911	arg1	4					861:861	4	861:861	4	861:861	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	2	59	theme	Psidium	358:364	arg1	polysaccharides					374:388	Psidium guajava polysaccharides	358:388	Psidium guajava polysaccharides	358:388	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	4	60	theme	carbohydrate	1091:1102	arg1	chains					1104:1109	long, branched, and complicated carbohydrate chains	1059:1109	long, branched, and complicated carbohydrate chains	1059:1109	This orthogonal one-pot glycosylation strategy can streamline the chemical synthesis of long, branched, and complicated carbohydrate chains.					
32086860	3	61	theme	Araf	780:783	arg1	motif					785:789	the challenging 2,3,5-branched Araf motif	749:789	the challenging 2,3,5-branched Araf motif	749:789	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	1	62	from	task	218:221	arg1	synthesis					235:243	chemical synthesis	226:243	chemical synthesis	226:243	The synthesis of long, branched, and complex carbohydrate sequences remains a challenging task in chemical synthesis.					
32086860	1	63	theme	long	145:148	arg1	sequences					186:194	long, branched, and complex carbohydrate sequences	145:194	long, branched, and complex carbohydrate sequences	145:194	The synthesis of long, branched, and complex carbohydrate sequences remains a challenging task in chemical synthesis.					
32086860	4	64	theme	complicated	1079:1089	arg1	chains					1104:1109	long, branched, and complicated carbohydrate chains	1059:1109	long, branched, and complicated carbohydrate chains	1059:1109	This orthogonal one-pot glycosylation strategy can streamline the chemical synthesis of long, branched, and complicated carbohydrate chains.					
32086860	3	65	theme	 the	794:797	arg1	1,2-cis-glucosylation					815:835	 the stereoselective 1,2-cis-glucosylation	794:835	3) the stereoselective 1,2-cis-glucosylation by reagent control	792:854	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	66	theme	target	943:948	arg1	nona-decasaccharide					950:968	the target nona-decasaccharide	939:968	the target nona-decasaccharide	939:968	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	2	67	theme	efficient	266:274	arg1	synthesis					296:304	an efficient and modular one-pot synthesis	263:304	an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity	263:445	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	2	68	theme	sequences	343:351	arg1	synthesis					296:304	an efficient and modular one-pot synthesis	263:304	an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity	263:445	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	3	69	theme	stereoselective	799:813	arg1	1,2-cis-glucosylation					815:835	 the stereoselective 1,2-cis-glucosylation	794:835	3) the stereoselective 1,2-cis-glucosylation by reagent control	792:854	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	4	70	theme	orthogonal	976:985	arg1	strategy					1009:1016	This orthogonal one-pot glycosylation strategy	971:1016	This orthogonal one-pot glycosylation strategy	971:1016	This orthogonal one-pot glycosylation strategy can streamline the chemical synthesis of long, branched, and complicated carbohydrate chains.					
32086860	3	71	theme	first	708:712	arg1	O5					726:727	first O3', second O5'	708:728	O5	726:727	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	72	theme	second	719:724	arg1	O5					726:727	first O3', second O5'	708:728	O5	726:727	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	73	theme	one-pot	492:498	arg1	reactions					514:522	 several one-pot glycosylation reactions	483:522	 several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation	483:596	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	1	74	theme	branched	151:158	arg1	sequences					186:194	long, branched, and complex carbohydrate sequences	145:194	long, branched, and complex carbohydrate sequences	145:194	The synthesis of long, branched, and complex carbohydrate sequences remains a challenging task in chemical synthesis.					
32086860	2	75	theme	α-glucosidase	413:425	arg1	activity					438:445	the potent α-glucosidase inhibitory activity	402:445	the potent α-glucosidase inhibitory activity	402:445	Reported here is an efficient and modular one-pot synthesis of a nona-decasaccharide and shorter sequences from Psidium guajava polysaccharides, which have the potent α-glucosidase inhibitory activity.					
32086860	3	76	theme	PTFAI	570:574	arg1	basis					531:535	the basis	527:535	the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation	527:596	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	4	77	theme	branched	1065:1072	arg1	chains					1104:1109	long, branched, and complicated carbohydrate chains	1059:1109	long, branched, and complicated carbohydrate chains	1059:1109	This orthogonal one-pot glycosylation strategy can streamline the chemical synthesis of long, branched, and complicated carbohydrate chains.					
32086860	3	78	dep	sequences	697:705	arg1	O5					726:727	first O3', second O5'	708:728	O5	726:727	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
32086860	3	78	dep	sequences	697:705	arg1	2					656:656	2	656:656	2	656:656	The synthetic strategy features: 1) several one-pot glycosylation reactions on the basis of N-phenyltrifluoroacetimidate (PTFAI) and Yu glycosylation to streamline the chemical synthesis of oligosaccharides, 2) the successful and efficient assembly sequences (first O3', second O5', final O2') toward the challenging 2,3,5-branched Araf motif, 3) the stereoselective 1,2-cis-glucosylation by reagent control, and 4) the convergent [6+6+7] one-pot coupling reaction for the final assembly of the target nona-decasaccharide.					
34463614	5	0	theme	postfusion	753:762	arg1	conformations					764:776	the prefusion and postfusion conformations	735:776	conformations	764:776	While infection relies on this transition between the prefusion and postfusion conformations, there has yet to be a biophysical characterization reported for this rearrangement.					
34463614	12	1	theme	viral	1851:1855	arg1	particle					1857:1864	the viral particle	1847:1864	the viral particle	1847:1864	In contrast, in the absence of glycans, the viral particle would likely fail to enter the host.					
34463614	9	2	used	used	1410:1413	arg2	model					1373:1377	an all-atom model	1361:1377	an all-atom model with simplified energetics	1361:1404	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	11	3	theme	critical	1736:1743	arg1	opportunity					1745:1755	a critical opportunity	1734:1755	a critical opportunity for the fusion peptides to capture the host cell	1734:1804	We additionally show that this glycan-induced delay provides a critical opportunity for the fusion peptides to capture the host cell.					
34463614	3	4	theme	viral	507:511	arg1	genome					513:518	the viral genome	503:518	the viral genome	503:518	This conformational rearrangement leads to membrane fusion, which creates transmembrane pores through which the viral genome is passed to the host.					
34463614	10	5	theme	glycans	1598:1604	arg1	composition					1579:1589	the steric composition	1568:1589	the steric composition of the glycans	1568:1604	These simulations indicate that the steric composition of the glycans can induce a pause during the Spike protein conformational change.					
34463614	13	6	theme	glycosylation	1933:1945	arg1	state					1947:1951	the glycosylation state	1929:1951	the glycosylation state	1929:1951	This analysis reveals how the glycosylation state can regulate infectivity, while providing a much-needed structural framework for studying the dynamics of this pervasive pathogen.					
34463614	9	7	with	model	1373:1377	arg1	energetics					1395:1404	simplified energetics	1384:1404	simplified energetics	1384:1404	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	2	8	theme	host	289:292	arg1	invasion					299:306	host cell invasion	289:306	host cell invasion	289:306	During transmission, host cell invasion is controlled by a large-scale (200-300 Å) conformational change of the Spike protein.					
34463614	13	9	theme	structural	2009:2018	arg1	framework					2020:2028	a much-needed structural framework	1995:2028	a much-needed structural framework for studying the dynamics of this pervasive pathogen	1995:2081	This analysis reveals how the glycosylation state can regulate infectivity, while providing a much-needed structural framework for studying the dynamics of this pervasive pathogen.					
34463614	8	10	theme	current	1153:1159	arg1	lack					1161:1164	the current lack	1149:1164	the current lack of data on the pre-to-post transition	1149:1202	With the current lack of data on the pre-to-post transition, the precise role of glycans during cell invasion has also remained unclear.					
34463614	4	11	theme	fusion	585:590	arg1	peptides					592:599	the fusion peptides	581:599	the fusion peptides	581:599	During Spike-protein-mediated fusion, the fusion peptides must be released from the core of the protein and associate with the host membrane.					
34463614	7	12	contain	possesses	1023:1031	arg1	protein					1015:1021	the Spike protein	1005:1021	the Spike protein	1005:1021	Interestingly, the Spike protein possesses many post-translational modifications, in the form of branched glycans that flank the surface of the assembly.					
34463614	7	12	contain	possesses	1023:1031	arg2	modifications					1057:1069	many post-translational modifications	1033:1069	many post-translational modifications	1033:1069	Interestingly, the Spike protein possesses many post-translational modifications, in the form of branched glycans that flank the surface of the assembly.					
34463614	9	13	theme	initial	1295:1301	arg1	description					1315:1325	an initial mechanistic description	1292:1325	an initial mechanistic description of the pre-to-post rearrangement	1292:1358	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	8	14	theme	data	1169:1172	arg1	lack					1161:1164	the current lack	1149:1164	the current lack of data on the pre-to-post transition	1149:1202	With the current lack of data on the pre-to-post transition, the precise role of glycans during cell invasion has also remained unclear.					
34463614	9	15	theme	protein	1464:1470	arg1	transitions					1472:1482	the protein transitions	1460:1482	the protein transitions between the prefusion and postfusion conformations	1460:1533	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	2	16	theme	Spike	380:384	arg1	protein					386:392	the Spike protein	376:392	the Spike protein	376:392	During transmission, host cell invasion is controlled by a large-scale (200-300 Å) conformational change of the Spike protein.					
34463614	8	17	theme	precise	1209:1215	arg1	role					1217:1220	the precise role	1205:1220	the precise role of glycans during cell invasion	1205:1252	With the current lack of data on the pre-to-post transition, the precise role of glycans during cell invasion has also remained unclear.					
34463614	9	18	theme	mechanistic	1303:1313	arg1	description					1315:1325	an initial mechanistic description	1292:1325	an initial mechanistic description of the pre-to-post rearrangement	1292:1358	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	10	19	theme	protein	1642:1648	arg1	change					1665:1670	the Spike protein conformational change	1632:1670	the Spike protein conformational change	1632:1670	These simulations indicate that the steric composition of the glycans can induce a pause during the Spike protein conformational change.					
34463614	1	20	theme	Severe	86:91	arg1	SARS-CoV-2					135:144	SARS-CoV-2	135:144	SARS-CoV-2	135:144	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and transmission involves a series of processes that may be targeted by vaccines and therapeutics.					
34463614	1	20	theme	Severe	86:91	arg1	coronavirus					120:130	Severe acute respiratory syndrome coronavirus 2	86:132	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	86:145	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and transmission involves a series of processes that may be targeted by vaccines and therapeutics.					
34463614	3	21	theme	membrane	438:445	arg1	fusion					447:452	membrane fusion	438:452	membrane fusion	438:452	This conformational rearrangement leads to membrane fusion, which creates transmembrane pores through which the viral genome is passed to the host.					
34463614	0	22	theme	confined	11:18	arg1	rearrangements					20:33	Sterically confined rearrangements	0:33	Sterically confined rearrangements of SARS-CoV-2 Spike protein	0:61	Sterically confined rearrangements of SARS-CoV-2 Spike protein control cell invasion.					
34463614	9	23	theme	rearrangement	1346:1358	arg1	description					1315:1325	an initial mechanistic description	1292:1325	an initial mechanistic description of the pre-to-post rearrangement	1292:1358	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	7	24	theme	branched	1087:1094	arg1	glycans					1096:1102	branched glycans	1087:1102	branched glycans that flank the surface of the assembly	1087:1141	Interestingly, the Spike protein possesses many post-translational modifications, in the form of branched glycans that flank the surface of the assembly.					
34463614	10	25	theme	steric	1572:1577	arg1	composition					1579:1589	the steric composition	1568:1589	the steric composition of the glycans	1568:1604	These simulations indicate that the steric composition of the glycans can induce a pause during the Spike protein conformational change.					
34463614	1	26	theme	acute	93:97	arg1	SARS-CoV-2					135:144	SARS-CoV-2	135:144	SARS-CoV-2	135:144	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and transmission involves a series of processes that may be targeted by vaccines and therapeutics.					
34463614	1	26	theme	acute	93:97	arg1	coronavirus					120:130	Severe acute respiratory syndrome coronavirus 2	86:132	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	86:145	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and transmission involves a series of processes that may be targeted by vaccines and therapeutics.					
34463614	8	27	theme	cell	1240:1243	arg1	invasion					1245:1252	cell invasion	1240:1252	cell invasion	1240:1252	With the current lack of data on the pre-to-post transition, the precise role of glycans during cell invasion has also remained unclear.					
34463614	7	28	theme	glycans	1096:1102	arg1	form					1079:1082	the form	1075:1082	the form of branched glycans that flank the surface of the assembly	1075:1141	Interestingly, the Spike protein possesses many post-translational modifications, in the form of branched glycans that flank the surface of the assembly.					
34463614	1	29	theme	respiratory	99:109	arg1	SARS-CoV-2					135:144	SARS-CoV-2	135:144	SARS-CoV-2	135:144	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and transmission involves a series of processes that may be targeted by vaccines and therapeutics.					
34463614	1	29	theme	respiratory	99:109	arg1	coronavirus					120:130	Severe acute respiratory syndrome coronavirus 2	86:132	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	86:145	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and transmission involves a series of processes that may be targeted by vaccines and therapeutics.					
34463614	10	30	theme	conformational	1650:1663	arg1	change					1665:1670	the Spike protein conformational change	1632:1670	the Spike protein conformational change	1632:1670	These simulations indicate that the steric composition of the glycans can induce a pause during the Spike protein conformational change.					
34463614	11	31	theme	host	1796:1799	arg1	cell					1801:1804	the host cell	1792:1804	the host cell	1792:1804	We additionally show that this glycan-induced delay provides a critical opportunity for the fusion peptides to capture the host cell.					
34463614	1	32	theme	syndrome	111:118	arg1	SARS-CoV-2					135:144	SARS-CoV-2	135:144	SARS-CoV-2	135:144	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and transmission involves a series of processes that may be targeted by vaccines and therapeutics.					
34463614	1	32	theme	syndrome	111:118	arg1	coronavirus					120:130	Severe acute respiratory syndrome coronavirus 2	86:132	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	86:145	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and transmission involves a series of processes that may be targeted by vaccines and therapeutics.					
34463614	5	33	theme	biophysical	801:811	arg1	characterization					813:828	a biophysical characterization	799:828	a biophysical characterization reported for this rearrangement	799:860	While infection relies on this transition between the prefusion and postfusion conformations, there has yet to be a biophysical characterization reported for this rearrangement.					
34463614	9	34	theme	pre-to-post	1334:1344	arg1	rearrangement					1346:1358	the pre-to-post rearrangement	1330:1358	the pre-to-post rearrangement	1330:1358	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	2	35	theme	cell	294:297	arg1	invasion					299:306	host cell invasion	289:306	host cell invasion	289:306	During transmission, host cell invasion is controlled by a large-scale (200-300 Å) conformational change of the Spike protein.					
34463614	2	36	theme	conformational	351:364	arg1	Å					348:348	200-300 Å	340:348	200-300 Å	340:348	During transmission, host cell invasion is controlled by a large-scale (200-300 Å) conformational change of the Spike protein.					
34463614	2	36	theme	conformational	351:364	arg1	change					366:371	a large-scale (200-300 Å) conformational change	325:371	a large-scale (200-300 Å) conformational change of the Spike protein	325:392	During transmission, host cell invasion is controlled by a large-scale (200-300 Å) conformational change of the Spike protein.					
34463614	0	37	theme	Spike	49:53	arg1	protein					55:61	SARS-CoV-2 Spike protein	38:61	SARS-CoV-2 Spike protein	38:61	Sterically confined rearrangements of SARS-CoV-2 Spike protein control cell invasion.					
34463614	4	38	theme	Spike-protein-mediated	550:571	arg1	fusion					573:578	Spike-protein-mediated fusion	550:578	Spike-protein-mediated fusion	550:578	During Spike-protein-mediated fusion, the fusion peptides must be released from the core of the protein and associate with the host membrane.					
34463614	9	39	theme	prefusion	1496:1504	arg1	conformations					1521:1533	the prefusion and postfusion conformations	1492:1533	conformations	1521:1533	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	1	40	theme	processes	207:215	arg1	series					197:202	a series	195:202	a series of processes that may be targeted by vaccines and therapeutics	195:265	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious, and transmission involves a series of processes that may be targeted by vaccines and therapeutics.					
34463614	0	41	theme	SARS-CoV-2	38:47	arg1	protein					55:61	SARS-CoV-2 Spike protein	38:61	SARS-CoV-2 Spike protein	38:61	Sterically confined rearrangements of SARS-CoV-2 Spike protein control cell invasion.					
34463614	6	42	theme	conformational	940:953	arg1	processes					955:963	the intermediate conformational processes	923:963	the intermediate conformational processes	923:963	That is, structures are available for the endpoints, though the intermediate conformational processes have not been described.					
34463614	3	43	theme	conformational	400:413	arg1	rearrangement					415:427	This conformational rearrangement	395:427	This conformational rearrangement	395:427	This conformational rearrangement leads to membrane fusion, which creates transmembrane pores through which the viral genome is passed to the host.					
34463614	11	44	theme	glycan-induced	1704:1717	arg1	delay					1719:1723	this glycan-induced delay	1699:1723	this glycan-induced delay	1699:1723	We additionally show that this glycan-induced delay provides a critical opportunity for the fusion peptides to capture the host cell.					
34463614	9	45	from	transitions	1472:1482	arg1	simulations					1439:1449	simulations	1439:1449	simulations in which the protein transitions between the prefusion and postfusion conformations	1439:1533	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	2	46	theme	protein	386:392	arg1	Å					348:348	200-300 Å	340:348	200-300 Å	340:348	During transmission, host cell invasion is controlled by a large-scale (200-300 Å) conformational change of the Spike protein.					
34463614	2	46	theme	protein	386:392	arg1	change					366:371	a large-scale (200-300 Å) conformational change	325:371	a large-scale (200-300 Å) conformational change of the Spike protein	325:392	During transmission, host cell invasion is controlled by a large-scale (200-300 Å) conformational change of the Spike protein.					
34463614	7	47	theme	assembly	1134:1141	arg1	surface					1119:1125	the surface	1115:1125	the surface of the assembly	1115:1141	Interestingly, the Spike protein possesses many post-translational modifications, in the form of branched glycans that flank the surface of the assembly.					
34463614	6	48	theme	intermediate	927:938	arg1	processes					955:963	the intermediate conformational processes	923:963	the intermediate conformational processes	923:963	That is, structures are available for the endpoints, though the intermediate conformational processes have not been described.					
34463614	4	49	attach	released	609:616	arg2	peptides					592:599	the fusion peptides	581:599	the fusion peptides	581:599	During Spike-protein-mediated fusion, the fusion peptides must be released from the core of the protein and associate with the host membrane.					
34463614	4	49	attach	released	609:616	arg1	core					627:630	the core	623:630	the core of the protein	623:645	During Spike-protein-mediated fusion, the fusion peptides must be released from the core of the protein and associate with the host membrane.					
34463614	0	50	theme	protein	55:61	arg1	rearrangements					20:33	Sterically confined rearrangements	0:33	Sterically confined rearrangements of SARS-CoV-2 Spike protein	0:61	Sterically confined rearrangements of SARS-CoV-2 Spike protein control cell invasion.					
34463614	9	51	theme	all-atom	1364:1371	arg1	model					1373:1377	an all-atom model	1361:1377	an all-atom model with simplified energetics	1361:1404	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	12	52	theme	glycans	1838:1844	arg1	absence					1827:1833	the absence	1823:1833	the absence of glycans	1823:1844	In contrast, in the absence of glycans, the viral particle would likely fail to enter the host.					
34463614	2	53	theme	large-scale	327:337	arg1	Å					348:348	200-300 Å	340:348	200-300 Å	340:348	During transmission, host cell invasion is controlled by a large-scale (200-300 Å) conformational change of the Spike protein.					
34463614	2	53	theme	large-scale	327:337	arg1	change					366:371	a large-scale (200-300 Å) conformational change	325:371	a large-scale (200-300 Å) conformational change of the Spike protein	325:392	During transmission, host cell invasion is controlled by a large-scale (200-300 Å) conformational change of the Spike protein.					
34463614	7	54	theme	many	1033:1036	arg1	modifications					1057:1069	many post-translational modifications	1033:1069	many post-translational modifications	1033:1069	Interestingly, the Spike protein possesses many post-translational modifications, in the form of branched glycans that flank the surface of the assembly.					
34463614	7	55	theme	Spike	1009:1013	arg1	protein					1015:1021	the Spike protein	1005:1021	the Spike protein	1005:1021	Interestingly, the Spike protein possesses many post-translational modifications, in the form of branched glycans that flank the surface of the assembly.					
34463614	3	56	theme	transmembrane	469:481	arg1	pores					483:487	transmembrane pores	469:487	transmembrane pores through which the viral genome is passed to the host	469:540	This conformational rearrangement leads to membrane fusion, which creates transmembrane pores through which the viral genome is passed to the host.					
34463614	9	57	theme	simulations	1439:1449	arg1	thousands					1426:1434	thousands	1426:1434	thousands of simulations in which the protein transitions between the prefusion and postfusion conformations	1426:1533	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	7	58	theme	post-translational	1038:1055	arg1	modifications					1057:1069	many post-translational modifications	1033:1069	many post-translational modifications	1033:1069	Interestingly, the Spike protein possesses many post-translational modifications, in the form of branched glycans that flank the surface of the assembly.					
34463614	8	59	from	lack	1161:1164	arg1	transition					1193:1202	the pre-to-post transition	1177:1202	the pre-to-post transition	1177:1202	With the current lack of data on the pre-to-post transition, the precise role of glycans during cell invasion has also remained unclear.					
34463614	13	60	theme	pervasive	2064:2072	arg1	pathogen					2074:2081	this pervasive pathogen	2059:2081	this pervasive pathogen	2059:2081	This analysis reveals how the glycosylation state can regulate infectivity, while providing a much-needed structural framework for studying the dynamics of this pervasive pathogen.					
34463614	9	61	theme	postfusion	1510:1519	arg1	conformations					1521:1533	the prefusion and postfusion conformations	1492:1533	conformations	1521:1533	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	9	62	theme	simplified	1384:1393	arg1	energetics					1395:1404	simplified energetics	1384:1404	simplified energetics	1384:1404	To provide an initial mechanistic description of the pre-to-post rearrangement, an all-atom model with simplified energetics was used to perform thousands of simulations in which the protein transitions between the prefusion and postfusion conformations.					
34463614	5	63	theme	prefusion	739:747	arg1	conformations					764:776	the prefusion and postfusion conformations	735:776	conformations	764:776	While infection relies on this transition between the prefusion and postfusion conformations, there has yet to be a biophysical characterization reported for this rearrangement.					
34463614	4	64	theme	host	670:673	arg1	membrane					675:682	the host membrane	666:682	the host membrane	666:682	During Spike-protein-mediated fusion, the fusion peptides must be released from the core of the protein and associate with the host membrane.					
34463614	4	65	theme	protein	639:645	arg1	core					627:630	the core	623:630	the core of the protein	623:645	During Spike-protein-mediated fusion, the fusion peptides must be released from the core of the protein and associate with the host membrane.					
34463614	11	66	theme	fusion	1765:1770	arg1	peptides					1772:1779	the fusion peptides	1761:1779	the fusion peptides	1761:1779	We additionally show that this glycan-induced delay provides a critical opportunity for the fusion peptides to capture the host cell.					
34463614	0	67	theme	cell	71:74	arg1	invasion					76:83	cell invasion	71:83	cell invasion	71:83	Sterically confined rearrangements of SARS-CoV-2 Spike protein control cell invasion.					
34463614	13	68	theme	pathogen	2074:2081	arg1	dynamics					2047:2054	the dynamics	2043:2054	the dynamics of this pervasive pathogen	2043:2081	This analysis reveals how the glycosylation state can regulate infectivity, while providing a much-needed structural framework for studying the dynamics of this pervasive pathogen.					
34463614	8	69	theme	pre-to-post	1181:1191	arg1	transition					1193:1202	the pre-to-post transition	1177:1202	the pre-to-post transition	1177:1202	With the current lack of data on the pre-to-post transition, the precise role of glycans during cell invasion has also remained unclear.					
34463614	13	70	theme	much-needed	1997:2007	arg1	framework					2020:2028	a much-needed structural framework	1995:2028	a much-needed structural framework for studying the dynamics of this pervasive pathogen	1995:2081	This analysis reveals how the glycosylation state can regulate infectivity, while providing a much-needed structural framework for studying the dynamics of this pervasive pathogen.					
34463614	8	71	theme	glycans	1225:1231	arg1	role					1217:1220	the precise role	1205:1220	the precise role of glycans during cell invasion	1205:1252	With the current lack of data on the pre-to-post transition, the precise role of glycans during cell invasion has also remained unclear.					
34463614	10	72	theme	Spike	1636:1640	arg1	change					1665:1670	the Spike protein conformational change	1632:1670	the Spike protein conformational change	1632:1670	These simulations indicate that the steric composition of the glycans can induce a pause during the Spike protein conformational change.					
34424958	6	0	theme	follicular	1095:1104	arg1	lymphoma					1106:1113	follicular lymphoma	1095:1113	follicular lymphoma	1095:1113	Acquisition of CDR-located acceptor sites associates with mutations of epigenetic regulators and BCL2 translocations, indicating an origin shared with follicular lymphoma.					
34424958	10	1	theme	potential	1979:1987	arg1	it					1965:1966	it	1965:1966	it	1965:1966	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	10	1	theme	potential	1979:1987	arg1	target					2009:2014	a potential precise therapeutic target	1977:2014	a potential precise therapeutic target	1977:2014	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	1	2	theme	B	261:261	arg1	cells					263:267	normal B cells	254:267	normal B cells	254:267	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	10	3	theme	Ig	1828:1829	arg1	modification					1854:1865	a posttranslational modification	1834:1865	a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior	1834:1958	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	10	3	theme	Ig	1828:1829	arg1	Oligomannosylation					1796:1813	Oligomannosylation	1796:1813	Oligomannosylation of the tumor Ig	1796:1829	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	8	4	from	glycans	1422:1428	arg1	cells					1446:1450	the lymphoma cells	1433:1450	the lymphoma cells	1433:1450	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	1	5	theme	variable	168:175	arg1	region					177:182	the surface immunoglobulin (Ig) variable region	136:182	the surface immunoglobulin (Ig) variable region	136:182	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	10	6	theme	Ig	2062:2063	arg1	interaction					2065:2075	the tumor Ig interaction	2052:2075	the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages	2052:2124	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	10	7	theme	GCB-DLBCL	1902:1910	arg1	category					1912:1919	a distinct GCB-DLBCL category	1891:1919	a distinct GCB-DLBCL category with more aggressive clinical behavior	1891:1958	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	3	8	theme	oligomannose-type	564:580	arg1	structures					582:591	oligomannose-type structures	564:591	oligomannose-type structures	564:591	Mass spectrometry and X-ray crystallography demonstrate how the inserted glycans are stalled at oligomannose-type structures because they are buried in the CDR loops.					
34424958	5	9	theme	B-cell-like	829:839	arg1	Ig					847:848	the activated B-cell-like DLBCL Ig	815:848	the activated B-cell-like DLBCL Ig	815:848	This markedly contrasts with the activated B-cell-like DLBCL Ig, which rarely has sites in the CDR and does not seem to acquire oligomannose-type structures.					
34424958	9	10	theme	carbohydrate	1763:1774	arg1	domain					1788:1793	the DC-SIGN carbohydrate recognition domain	1751:1793	the DC-SIGN carbohydrate recognition domain	1751:1793	Both clustering and signaling are inhibited by antibodies specifically targeting the DC-SIGN carbohydrate recognition domain.					
34424958	7	11	theme	MYC	1380:1382	arg1	translocations					1384:1397	MYC translocations	1380:1397	MYC translocations	1380:1397	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	10	12	theme	M2-polarized	2101:2112	arg1	macrophages					2114:2124	DC-SIGN-expressing M2-polarized macrophages	2082:2124	DC-SIGN-expressing M2-polarized macrophages	2082:2124	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	1	13	theme	lymphoma-associated	211:229	arg1	feature					231:237	a remarkable follicular lymphoma-associated feature	187:237	a remarkable follicular lymphoma-associated feature rarely seen in normal B cells	187:267	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	1	13	theme	lymphoma-associated	211:229	arg1	Glycosylation					119:131	Glycosylation	119:131	Glycosylation of the surface immunoglobulin (Ig) variable region	119:182	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	7	14	theme	devoid	1370:1375	arg1	fraction					1361:1368	the fraction	1357:1368	the fraction devoid of MYC translocations	1357:1397	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	10	15	theme	tumor	1822:1826	arg1	Ig					1828:1829	the tumor Ig	1818:1829	the tumor Ig	1818:1829	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	8	16	theme	lectin	1480:1485	arg1	DC-SIGN					1568:1574	DC-SIGN	1568:1574	DC-SIGN	1568:1574	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	8	16	theme	lectin	1480:1485	arg1	molecule					1534:1541	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin	1466:1565	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN)	1466:1575	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	6	17	theme	epigenetic	1015:1024	arg1	regulators					1026:1035	epigenetic regulators	1015:1035	epigenetic regulators	1015:1035	Acquisition of CDR-located acceptor sites associates with mutations of epigenetic regulators and BCL2 translocations, indicating an origin shared with follicular lymphoma.					
34424958	7	18	from	enrichment	1233:1242	arg1	fraction					1361:1368	the fraction	1357:1368	the fraction devoid of MYC translocations	1357:1397	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	4	19	theme	B-cell-like	690:700	arg1	DLBCL					702:706	germinal-center B-cell-like DLBCL	674:706	germinal-center B-cell-like DLBCL (GCB-DLBCL)	674:718	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	4	19	theme	B-cell-like	690:700	arg1	GCB-DLBCL					709:717	GCB-DLBCL	709:717	GCB-DLBCL	709:717	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	10	20	gly	Oligomannosylation	1796:1813	arg1	Ig					1828:1829	the tumor Ig	1818:1829	the tumor Ig	1818:1829	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	3	21	theme	CDR	624:626	arg1	loops					628:632	the CDR loops	620:632	the CDR loops	620:632	Mass spectrometry and X-ray crystallography demonstrate how the inserted glycans are stalled at oligomannose-type structures because they are buried in the CDR loops.					
34424958	1	22	theme	immunoglobulin	148:161	arg1	region					177:182	the surface immunoglobulin (Ig) variable region	136:182	the surface immunoglobulin (Ig) variable region	136:182	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	8	23	theme	adhesion	1525:1532	arg1	DC-SIGN					1568:1574	DC-SIGN	1568:1574	DC-SIGN	1568:1574	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	8	23	theme	adhesion	1525:1532	arg1	molecule					1534:1541	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin	1466:1565	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN)	1466:1575	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	7	24	theme	gene	1224:1227	arg1	enrichment					1233:1242	significant gene set enrichment	1212:1242	significant gene set enrichment	1212:1242	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	0	25	theme	distinct	89:96	arg1	origin					98:103	distinct origin	89:103	distinct origin	89:103	Insertion of atypical glycans into the tumor antigen-binding site identifies DLBCLs with distinct origin and behavior.					
34424958	5	26	from	sites	868:872	arg1	CDR					881:883	the CDR	877:883	the CDR	877:883	This markedly contrasts with the activated B-cell-like DLBCL Ig, which rarely has sites in the CDR and does not seem to acquire oligomannose-type structures.					
34424958	6	27	theme	acceptor	971:978	arg1	sites					980:984	CDR-located acceptor sites	959:984	CDR-located acceptor sites	959:984	Acquisition of CDR-located acceptor sites associates with mutations of epigenetic regulators and BCL2 translocations, indicating an origin shared with follicular lymphoma.					
34424958	8	28	theme	non-integrin	1554:1565	arg1	DC-SIGN					1568:1574	DC-SIGN	1568:1574	DC-SIGN	1568:1574	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	8	28	theme	non-integrin	1554:1565	arg1	molecule					1534:1541	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin	1466:1565	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN)	1466:1575	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	2	29	gly	N-glycosylation	351:365	arg2	sites					367:371	N-glycosylation sites	351:371	N-glycosylation sites	351:371	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	7	30	theme	rapid	1177:1181	arg1	progression					1191:1201	more rapid disease progression	1172:1201	more rapid disease progression	1172:1201	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	8	31	theme	cell-specific	1497:1509	arg1	DC-SIGN					1568:1574	DC-SIGN	1568:1574	DC-SIGN	1568:1574	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	8	31	theme	cell-specific	1497:1509	arg1	molecule					1534:1541	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin	1466:1565	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN)	1466:1575	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	7	32	theme	signaling	1321:1329	arg1	pathways					1331:1338	MYC signaling pathways	1317:1338	MYC signaling pathways	1317:1338	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	4	33	theme	EZB	743:745	arg1	subtype					747:753	the genetic EZB subtype	731:753	the genetic EZB subtype	731:753	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	0	34	theme	atypical	13:20	arg1	glycans					22:28	atypical glycans	13:28	atypical glycans	13:28	Insertion of atypical glycans into the tumor antigen-binding site identifies DLBCLs with distinct origin and behavior.					
34424958	8	35	with	clusters	1640:1647	arg1	cells					1663:1667	lymphoma cells	1654:1667	lymphoma cells	1654:1667	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	6	36	theme	translocations	1046:1059	arg1	mutations					1002:1010	mutations	1002:1010	mutations of epigenetic regulators and BCL2 translocations	1002:1059	Acquisition of CDR-located acceptor sites associates with mutations of epigenetic regulators and BCL2 translocations, indicating an origin shared with follicular lymphoma.					
34424958	8	37	theme	low-level	1588:1596	arg1	signals					1598:1604	low-level signals	1588:1604	low-level signals	1588:1604	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	6	38	theme	BCL2	1041:1044	arg1	translocations					1046:1059	BCL2 translocations	1041:1059	BCL2 translocations	1041:1059	Acquisition of CDR-located acceptor sites associates with mutations of epigenetic regulators and BCL2 translocations, indicating an origin shared with follicular lymphoma.					
34424958	2	39	theme	Ig	392:393	arg1	CDRs					432:435	CDRs	432:435	CDRs	432:435	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	2	39	theme	Ig	392:393	arg1	regions					423:429	Ig complementarity-determining regions	392:429	the Ig complementarity-determining regions (CDRs) of the antigen-binding sites	388:465	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	7	40	theme	metabolism	1301:1310	arg1	enrichment					1233:1242	significant gene set enrichment	1212:1242	significant gene set enrichment	1212:1242	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	2	41	theme	antigen-binding	445:459	arg1	sites					461:465	the antigen-binding sites	441:465	the antigen-binding sites	441:465	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	10	42	theme	aggressive	1931:1940	arg1	behavior					1951:1958	more aggressive clinical behavior	1926:1958	more aggressive clinical behavior	1926:1958	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	0	43	theme	tumor	39:43	arg1	site					61:64	the tumor antigen-binding site	35:64	the tumor antigen-binding site	35:64	Insertion of atypical glycans into the tumor antigen-binding site identifies DLBCLs with distinct origin and behavior.					
34424958	8	44	theme	lectin-expressing	1611:1627	arg1	cells					1629:1633	lectin-expressing cells	1611:1633	lectin-expressing cells	1611:1633	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	4	45	theme	use	781:783	arg1	irrespective					756:767	irrespective	756:767	irrespective	756:767	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	2	46	theme	B-cell	312:317	arg1	lymphomas					319:327	diffuse large B-cell lymphomas	298:327	diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites	298:465	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	2	46	theme	B-cell	312:317	arg1	DLBCLs					330:335	DLBCLs	330:335	DLBCLs	330:335	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	7	47	theme	PI3K/AKT/MTORC1	1268:1282	arg1	pathway					1284:1290	PI3K/AKT/MTORC1 pathway	1268:1290	PI3K/AKT/MTORC1 pathway	1268:1290	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	7	48	theme	receptor	1258:1265	arg1	enrichment					1233:1242	significant gene set enrichment	1212:1242	significant gene set enrichment	1212:1242	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	7	49	theme	EZB	1127:1129	arg1	subtype					1131:1137	the EZB subtype	1123:1137	the EZB subtype	1123:1137	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	1	50	theme	normal	254:259	arg1	cells					263:267	normal B cells	254:267	normal B cells	254:267	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	0	51	with	DLBCLs	77:82	arg1	origin					98:103	distinct origin	89:103	distinct origin	89:103	Insertion of atypical glycans into the tumor antigen-binding site identifies DLBCLs with distinct origin and behavior.					
34424958	0	51	with	DLBCLs	77:82	arg1	behavior					109:116	behavior	109:116	behavior	109:116	Insertion of atypical glycans into the tumor antigen-binding site identifies DLBCLs with distinct origin and behavior.					
34424958	10	52	theme	antibody-mediated	2020:2036	arg1	inhibition					2038:2047	antibody-mediated inhibition	2020:2047	antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages	2020:2124	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	3	53	theme	inserted	532:539	arg1	glycans					541:547	the inserted glycans	528:547	the inserted glycans	528:547	Mass spectrometry and X-ray crystallography demonstrate how the inserted glycans are stalled at oligomannose-type structures because they are buried in the CDR loops.					
34424958	2	54	theme	diffuse	298:304	arg1	lymphomas					319:327	diffuse large B-cell lymphomas	298:327	diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites	298:465	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	2	54	theme	diffuse	298:304	arg1	DLBCLs					330:335	DLBCLs	330:335	DLBCLs	330:335	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	1	55	theme	region	177:182	arg1	feature					231:237	a remarkable follicular lymphoma-associated feature	187:237	a remarkable follicular lymphoma-associated feature rarely seen in normal B cells	187:267	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	1	55	theme	region	177:182	arg1	Glycosylation					119:131	Glycosylation	119:131	Glycosylation of the surface immunoglobulin (Ig) variable region	119:182	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	6	56	theme	CDR-located	959:969	arg1	sites					980:984	CDR-located acceptor sites	959:984	CDR-located acceptor sites	959:984	Acquisition of CDR-located acceptor sites associates with mutations of epigenetic regulators and BCL2 translocations, indicating an origin shared with follicular lymphoma.					
34424958	10	57	theme	tumor	2056:2060	arg1	interaction					2065:2075	the tumor Ig interaction	2052:2075	the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages	2052:2124	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	1	58	gly	Glycosylation	119:131	arg1	region					177:182	the surface immunoglobulin (Ig) variable region	136:182	the surface immunoglobulin (Ig) variable region	136:182	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	10	59	theme	posttranslational	1836:1852	arg1	Oligomannosylation					1796:1813	Oligomannosylation	1796:1813	Oligomannosylation of the tumor Ig	1796:1829	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	10	59	theme	posttranslational	1836:1852	arg1	modification					1854:1865	a posttranslational modification	1834:1865	a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior	1834:1958	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	5	60	theme	activated	819:827	arg1	Ig					847:848	the activated B-cell-like DLBCL Ig	815:848	the activated B-cell-like DLBCL Ig	815:848	This markedly contrasts with the activated B-cell-like DLBCL Ig, which rarely has sites in the CDR and does not seem to acquire oligomannose-type structures.					
34424958	9	61	theme	DC-SIGN	1755:1761	arg1	domain					1788:1793	the DC-SIGN carbohydrate recognition domain	1751:1793	the DC-SIGN carbohydrate recognition domain	1751:1793	Both clustering and signaling are inhibited by antibodies specifically targeting the DC-SIGN carbohydrate recognition domain.					
34424958	2	62	theme	lymphomas	319:327	arg1	subset					288:293	a subset	286:293	a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites	286:465	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	10	63	theme	distinct	1893:1900	arg1	category					1912:1919	a distinct GCB-DLBCL category	1891:1919	a distinct GCB-DLBCL category with more aggressive clinical behavior	1891:1958	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	5	64	theme	DLBCL	841:845	arg1	Ig					847:848	the activated B-cell-like DLBCL Ig	815:848	the activated B-cell-like DLBCL Ig	815:848	This markedly contrasts with the activated B-cell-like DLBCL Ig, which rarely has sites in the CDR and does not seem to acquire oligomannose-type structures.					
34424958	10	65	with	category	1912:1919	arg1	behavior					1951:1958	more aggressive clinical behavior	1926:1958	more aggressive clinical behavior	1926:1958	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	9	66	theme	recognition	1776:1786	arg1	domain					1788:1793	the DC-SIGN carbohydrate recognition domain	1751:1793	the DC-SIGN carbohydrate recognition domain	1751:1793	Both clustering and signaling are inhibited by antibodies specifically targeting the DC-SIGN carbohydrate recognition domain.					
34424958	8	67	theme	candidate	1470:1478	arg1	DC-SIGN					1568:1574	DC-SIGN	1568:1574	DC-SIGN	1568:1574	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	8	67	theme	candidate	1470:1478	arg1	molecule					1534:1541	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin	1466:1565	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN)	1466:1575	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	7	68	theme	translocations	1384:1397	arg1	devoid					1370:1375	devoid	1370:1375	devoid	1370:1375	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	4	69	theme	sites	650:654	arg1	Acquisition					635:645	Acquisition	635:645	Acquisition of sites	635:654	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	10	70	theme	DC-SIGN-expressing	2082:2099	arg1	macrophages					2114:2124	DC-SIGN-expressing M2-polarized macrophages	2082:2124	DC-SIGN-expressing M2-polarized macrophages	2082:2124	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	8	71	theme	dendritic	1487:1495	arg1	DC-SIGN					1568:1574	DC-SIGN	1568:1574	DC-SIGN	1568:1574	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	8	71	theme	dendritic	1487:1495	arg1	molecule					1534:1541	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin	1466:1565	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN)	1466:1575	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	10	72	theme	interaction	2065:2075	arg1	inhibition					2038:2047	antibody-mediated inhibition	2020:2047	antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages	2020:2124	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	6	73	theme	regulators	1026:1035	arg1	mutations					1002:1010	mutations	1002:1010	mutations of epigenetic regulators and BCL2 translocations	1002:1059	Acquisition of CDR-located acceptor sites associates with mutations of epigenetic regulators and BCL2 translocations, indicating an origin shared with follicular lymphoma.					
34424958	8	74	theme	intercellular	1511:1523	arg1	DC-SIGN					1568:1574	DC-SIGN	1568:1574	DC-SIGN	1568:1574	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	8	74	theme	intercellular	1511:1523	arg1	molecule					1534:1541	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin	1466:1565	the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN)	1466:1575	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	10	75	with	interaction	2065:2075	arg1	macrophages					2114:2124	DC-SIGN-expressing M2-polarized macrophages	2082:2124	DC-SIGN-expressing M2-polarized macrophages	2082:2124	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	3	76	theme	Mass	468:471	arg1	spectrometry					473:484	Mass spectrometry	468:484	Mass spectrometry	468:484	Mass spectrometry and X-ray crystallography demonstrate how the inserted glycans are stalled at oligomannose-type structures because they are buried in the CDR loops.					
34424958	7	77	theme	set	1229:1231	arg1	enrichment					1233:1242	significant gene set enrichment	1212:1242	significant gene set enrichment	1212:1242	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	1	78	theme	surface	140:146	arg1	immunoglobulin					148:161	surface immunoglobulin	140:161	the surface immunoglobulin (Ig) variable region	136:182	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	1	78	theme	surface	140:146	arg1	Ig					164:165	Ig	164:165	Ig	164:165	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	4	79	theme	germinal-center	674:688	arg1	DLBCL					702:706	germinal-center B-cell-like DLBCL	674:706	germinal-center B-cell-like DLBCL (GCB-DLBCL)	674:718	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	4	79	theme	germinal-center	674:688	arg1	GCB-DLBCL					709:717	GCB-DLBCL	709:717	GCB-DLBCL	709:717	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	7	80	theme	significant	1212:1222	arg1	enrichment					1233:1242	significant gene set enrichment	1212:1242	significant gene set enrichment	1212:1242	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	5	81	contain	has	864:866	arg2	sites					868:872	sites	868:872	sites in the CDR	868:883	This markedly contrasts with the activated B-cell-like DLBCL Ig, which rarely has sites in the CDR and does not seem to acquire oligomannose-type structures.					
34424958	5	81	contain	has	864:866	arg1	Ig					847:848	the activated B-cell-like DLBCL Ig	815:848	the activated B-cell-like DLBCL Ig	815:848	This markedly contrasts with the activated B-cell-like DLBCL Ig, which rarely has sites in the CDR and does not seem to acquire oligomannose-type structures.					
34424958	4	82	theme	DLBCL	702:706	arg1	DLBCL					702:706	germinal-center B-cell-like DLBCL	674:706	germinal-center B-cell-like DLBCL (GCB-DLBCL)	674:718	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	4	82	theme	DLBCL	702:706	arg1	%					669:669	∼50%	666:669	∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL)	666:718	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	4	82	theme	DLBCL	702:706	arg1	GCB-DLBCL					709:717	GCB-DLBCL	709:717	GCB-DLBCL	709:717	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	6	83	theme	sites	980:984	arg1	Acquisition					944:954	Acquisition	944:954	Acquisition of CDR-located acceptor sites	944:984	Acquisition of CDR-located acceptor sites associates with mutations of epigenetic regulators and BCL2 translocations, indicating an origin shared with follicular lymphoma.					
34424958	7	84	theme	disease	1183:1189	arg1	progression					1191:1201	more rapid disease progression	1172:1201	more rapid disease progression	1172:1201	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	1	85	theme	remarkable	189:198	arg1	feature					231:237	a remarkable follicular lymphoma-associated feature	187:237	a remarkable follicular lymphoma-associated feature rarely seen in normal B cells	187:267	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	1	85	theme	remarkable	189:198	arg1	Glycosylation					119:131	Glycosylation	119:131	Glycosylation of the surface immunoglobulin (Ig) variable region	119:182	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	7	86	theme	pathways	1331:1338	arg1	enrichment					1233:1242	significant gene set enrichment	1212:1242	significant gene set enrichment	1212:1242	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	7	87	theme	MYC	1317:1319	arg1	pathways					1331:1338	MYC signaling pathways	1317:1338	MYC signaling pathways	1317:1338	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	2	88	theme	N-glycosylation	351:365	arg1	sites					367:371	N-glycosylation sites	351:371	N-glycosylation sites	351:371	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	0	89	theme	glycans	22:28	arg1	Insertion					0:8	Insertion	0:8	Insertion of atypical glycans into the tumor antigen-binding site	0:64	Insertion of atypical glycans into the tumor antigen-binding site identifies DLBCLs with distinct origin and behavior.					
34424958	5	90	theme	oligomannose-type	914:930	arg1	structures					932:941	oligomannose-type structures	914:941	oligomannose-type structures	914:941	This markedly contrasts with the activated B-cell-like DLBCL Ig, which rarely has sites in the CDR and does not seem to acquire oligomannose-type structures.					
34424958	4	91	theme	genetic	735:741	arg1	subtype					747:753	the genetic EZB subtype	731:753	the genetic EZB subtype	731:753	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	2	92	theme	complementarity-determining	395:421	arg1	CDRs					432:435	CDRs	432:435	CDRs	432:435	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	2	92	theme	complementarity-determining	395:421	arg1	regions					423:429	Ig complementarity-determining regions	392:429	the Ig complementarity-determining regions (CDRs) of the antigen-binding sites	388:465	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	1	93	theme	follicular	200:209	arg1	feature					231:237	a remarkable follicular lymphoma-associated feature	187:237	a remarkable follicular lymphoma-associated feature rarely seen in normal B cells	187:267	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	1	93	theme	follicular	200:209	arg1	Glycosylation					119:131	Glycosylation	119:131	Glycosylation of the surface immunoglobulin (Ig) variable region	119:182	Glycosylation of the surface immunoglobulin (Ig) variable region is a remarkable follicular lymphoma-associated feature rarely seen in normal B cells.					
34424958	7	94	theme	glucose	1293:1299	arg1	metabolism					1301:1310	glucose metabolism	1293:1310	glucose metabolism	1293:1310	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	2	95	theme	sites	461:465	arg1	CDRs					432:435	CDRs	432:435	CDRs	432:435	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	2	95	theme	sites	461:465	arg1	regions					423:429	Ig complementarity-determining regions	392:429	the Ig complementarity-determining regions (CDRs) of the antigen-binding sites	388:465	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	10	96	dep	potential	1979:1987	arg1	therapeutic					1997:2007	therapeutic	1997:2007	therapeutic	1997:2007	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	10	97	theme	clinical	1942:1949	arg1	behavior					1951:1958	more aggressive clinical behavior	1926:1958	more aggressive clinical behavior	1926:1958	Oligomannosylation of the tumor Ig is a posttranslational modification that readily identifies a distinct GCB-DLBCL category with more aggressive clinical behavior, and it could be a potential precise therapeutic target via antibody-mediated inhibition of the tumor Ig interaction with DC-SIGN-expressing M2-polarized macrophages.					
34424958	0	98	theme	antigen-binding	45:59	arg1	site					61:64	the tumor antigen-binding site	35:64	the tumor antigen-binding site	35:64	Insertion of atypical glycans into the tumor antigen-binding site identifies DLBCLs with distinct origin and behavior.					
34424958	7	99	theme	pathway	1284:1290	arg1	enrichment					1233:1242	significant gene set enrichment	1212:1242	significant gene set enrichment	1212:1242	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	8	100	theme	oligomannose-type	1404:1420	arg1	glycans					1422:1428	The oligomannose-type glycans	1400:1428	The oligomannose-type glycans on the lymphoma cells	1400:1450	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	3	101	theme	X-ray	490:494	arg1	crystallography					496:510	X-ray crystallography	490:510	X-ray crystallography	490:510	Mass spectrometry and X-ray crystallography demonstrate how the inserted glycans are stalled at oligomannose-type structures because they are buried in the CDR loops.					
34424958	4	102	theme	IGHV-D-J	772:779	arg1	use					781:783	IGHV-D-J use	772:783	IGHV-D-J use	772:783	Acquisition of sites occurs in ∼50% of germinal-center B-cell-like DLBCL (GCB-DLBCL), mainly of the genetic EZB subtype, irrespective of IGHV-D-J use.					
34424958	2	103	theme	large	306:310	arg1	lymphomas					319:327	diffuse large B-cell lymphomas	298:327	diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites	298:465	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	2	103	theme	large	306:310	arg1	DLBCLs					330:335	DLBCLs	330:335	DLBCLs	330:335	Here, we define a subset of diffuse large B-cell lymphomas (DLBCLs) that acquire N-glycosylation sites selectively in the Ig complementarity-determining regions (CDRs) of the antigen-binding sites.					
34424958	7	104	theme	B-cell	1251:1256	arg1	receptor					1258:1265	the B-cell receptor	1247:1265	the B-cell receptor	1247:1265	Within the EZB subtype, these sites are associated with more rapid disease progression and with significant gene set enrichment of the B-cell receptor, PI3K/AKT/MTORC1 pathway, glucose metabolism, and MYC signaling pathways, particularly in the fraction devoid of MYC translocations.					
34424958	8	105	theme	lymphoma	1654:1661	arg1	cells					1663:1667	lymphoma cells	1654:1667	lymphoma cells	1654:1667	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
34424958	8	106	theme	lymphoma	1437:1444	arg1	cells					1446:1450	the lymphoma cells	1433:1450	the lymphoma cells	1433:1450	The oligomannose-type glycans on the lymphoma cells interact with the candidate lectin dendritic cell-specific intercellular adhesion molecule 3 grabbing non-integrin (DC-SIGN), mediating low-level signals, and lectin-expressing cells form clusters with lymphoma cells.					
32749397	4	0	theme	total	905:909	arg1	content					920:926	the total N-glycan content	901:926	the total N-glycan content	901:926	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	1	1	theme	protein	182:188	arg1	biotherapeutics					190:204	protein biotherapeutics	182:204	protein biotherapeutics	182:204	Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety.					
32749397	4	2	theme	released	613:620	arg1	glycans					622:628	released glycans	613:628	released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy	613:734	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	0	3	theme	mobility	87:94	arg1	spectrometry					96:107	ultrahigh-resolution ion mobility spectrometry	62:107	ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy	62:148	Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy.					
32749397	6	4	link	N-linked	1124:1131	arg1	glycans					1133:1139	N-linked glycans	1124:1139	N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms	1124:1261	This work represents an important advance in the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms.					
32749397	5	5	theme	recorded	933:940	arg1	spectra					954:960	The recorded vibrational spectra	929:960	The recorded vibrational spectra	929:960	The recorded vibrational spectra exhibit well-resolved transitions that can be used as a fingerprint to identify a particular glycan.					
32749397	5	6	theme	vibrational	942:952	arg1	spectra					954:960	The recorded vibrational spectra	929:960	The recorded vibrational spectra	929:960	The recorded vibrational spectra exhibit well-resolved transitions that can be used as a fingerprint to identify a particular glycan.					
32749397	4	7	theme	content	920:926	arg1	%					896:896	1% to 22%	888:896	1% to 22% of the total N-glycan content	888:926	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	4	7	theme	content	920:926	arg1	content					920:926	the total N-glycan content	901:926	the total N-glycan content	901:926	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	4	8	theme	therapeutic	821:831	arg1	range					864:868	the therapeutic fusion protein etanercept that range	817:868	the therapeutic fusion protein etanercept that range in abundance	817:881	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	4	9	theme	N-glycan	911:918	arg1	content					920:926	the total N-glycan content	901:926	the total N-glycan content	901:926	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	2	10	theme	adequate	373:380	arg1	approach					393:400	an adequate analytical approach	370:400	an adequate analytical approach to guarantee product quality	370:429	As a result, glycosylation is categorized as a critical quality attribute that needs an adequate analytical approach to guarantee product quality.					
32749397	0	11	theme	ion	133:135	arg1	spectroscopy					137:148	cryogenic ion spectroscopy	123:148	cryogenic ion spectroscopy	123:148	Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy.					
32749397	1	12	attach	attached	170:177	arg1	biotherapeutics					190:204	protein biotherapeutics	182:204	protein biotherapeutics	182:204	Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety.					
32749397	1	12	attach	attached	170:177	arg2	Glycans					151:157	Glycans	151:157	Glycans covalently attached to protein biotherapeutics	151:204	Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety.					
32749397	4	13	theme	22	894:895	arg1	%					889:889	%	889:889	%	889:889	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	6	14	theme	N-linked	1124:1131	arg1	glycans					1133:1139	N-linked glycans	1124:1139	N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms	1124:1261	This work represents an important advance in the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms.					
32749397	4	15	theme	protein	840:846	arg1	range					864:868	the therapeutic fusion protein etanercept that range	817:868	the therapeutic fusion protein etanercept that range in abundance	817:881	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	1	16	theme	significant	213:223	arg1	impact					225:230	a significant impact	211:230	a significant impact on their biological activity, clearance, and safety	211:282	Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety.					
32749397	4	17	theme	cryogenic	701:709	arg1	spectroscopy					723:734	cryogenic vibrational spectroscopy	701:734	cryogenic vibrational spectroscopy	701:734	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	0	18	theme	cryogenic	123:131	arg1	spectroscopy					137:148	cryogenic ion spectroscopy	123:148	cryogenic ion spectroscopy	123:148	Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy.					
32749397	6	19	dep	important	1087:1095	arg1	advance					1097:1103	advance	1097:1103	advance in the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms	1097:1261	This work represents an important advance in the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms.					
32749397	4	20	theme	fusion	833:838	arg1	range					864:868	the therapeutic fusion protein etanercept that range	817:868	the therapeutic fusion protein etanercept that range in abundance	817:881	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	6	21	theme	glycans	1133:1139	arg1	analysis					1112:1119	the analysis	1108:1119	the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms	1108:1261	This work represents an important advance in the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms.					
32749397	2	22	theme	product	415:421	arg1	quality					423:429	product quality	415:429	product quality	415:429	As a result, glycosylation is categorized as a critical quality attribute that needs an adequate analytical approach to guarantee product quality.					
32749397	2	23	theme	critical	332:339	arg1	glycosylation					298:310	glycosylation	298:310	glycosylation	298:310	As a result, glycosylation is categorized as a critical quality attribute that needs an adequate analytical approach to guarantee product quality.					
32749397	2	23	theme	critical	332:339	arg1	attribute					349:357	a critical quality attribute	330:357	a critical quality attribute that needs an adequate analytical approach to guarantee product quality	330:429	As a result, glycosylation is categorized as a critical quality attribute that needs an adequate analytical approach to guarantee product quality.					
32749397	3	24	theme	isomeric	445:452	arg1	complexity					454:463	isomeric complexity	445:463	isomeric complexity	445:463	However, the isomeric complexity and branched structure of glycans makes their analysis a significant challenge.					
32749397	4	25	theme	multidimensional	572:587	arg1	approach					589:596	a multidimensional approach	570:596	a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content	570:926	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	4	26	theme	vibrational	711:721	arg1	spectroscopy					723:734	cryogenic vibrational spectroscopy	701:734	cryogenic vibrational spectroscopy	701:734	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	3	27	theme	glycans	491:497	arg1	complexity					454:463	isomeric complexity	445:463	isomeric complexity	445:463	However, the isomeric complexity and branched structure of glycans makes their analysis a significant challenge.					
32749397	3	27	theme	glycans	491:497	arg1	structure					478:486	branched structure	469:486	branched structure	469:486	However, the isomeric complexity and branched structure of glycans makes their analysis a significant challenge.					
32749397	4	28	from	range	864:868	arg1	abundance					873:881	abundance	873:881	abundance	873:881	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	4	29	dep	%	889:889	arg1	to					891:892	to	891:892	to	891:892	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	0	30	theme	biotherapeutic	33:46	arg1	protein					48:54	a biotherapeutic protein	31:54	a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy	31:148	Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy.					
32749397	6	31	used	used	1206:1209	arg2	tool					1214:1217	tool	1214:1217	tool for monitoring biopharmaceutical glycoforms	1214:1261	This work represents an important advance in the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms.					
32749397	6	31	used	used	1206:1209	arg2	proteins					1172:1179	biopharmaceutical proteins	1154:1179	biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms	1154:1261	This work represents an important advance in the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms.					
32749397	1	32	theme	biological	241:250	arg1	activity					252:259	their biological activity	235:259	their biological activity	235:259	Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety.					
32749397	5	33	used	used	1008:1011	arg2	transitions					984:994	well-resolved transitions	970:994	well-resolved transitions that can be used as a fingerprint to identify a particular glycan	970:1060	The recorded vibrational spectra exhibit well-resolved transitions that can be used as a fingerprint to identify a particular glycan.					
32749397	5	33	used	used	1008:1011	arg2	fingerprint					1018:1028	a fingerprint	1016:1028	a fingerprint	1016:1028	The recorded vibrational spectra exhibit well-resolved transitions that can be used as a fingerprint to identify a particular glycan.					
32749397	3	34	dep	complexity	454:463	arg1	the					441:443	the	441:443	the	441:443	However, the isomeric complexity and branched structure of glycans makes their analysis a significant challenge.					
32749397	1	35	contain	have	206:209	arg1	Glycans					151:157	Glycans	151:157	Glycans covalently attached to protein biotherapeutics	151:204	Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety.					
32749397	1	35	contain	have	206:209	arg2	impact					225:230	a significant impact	211:230	a significant impact on their biological activity, clearance, and safety	211:282	Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety.					
32749397	6	36	theme	biopharmaceutical	1234:1250	arg1	glycoforms					1252:1261	biopharmaceutical glycoforms	1234:1261	biopharmaceutical glycoforms	1234:1261	This work represents an important advance in the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms.					
32749397	4	37	theme	1	888:888	arg1	%					889:889	%	889:889	%	889:889	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	3	38	theme	significant	522:532	arg1	challenge					534:542	a significant challenge	520:542	a significant challenge	520:542	However, the isomeric complexity and branched structure of glycans makes their analysis a significant challenge.					
32749397	4	39	theme	that	859:862	arg1	range					864:868	the therapeutic fusion protein etanercept that range	817:868	the therapeutic fusion protein etanercept that range in abundance	817:881	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	5	40	theme	well-resolved	970:982	arg1	fingerprint					1018:1028	a fingerprint	1016:1028	a fingerprint	1016:1028	The recorded vibrational spectra exhibit well-resolved transitions that can be used as a fingerprint to identify a particular glycan.					
32749397	5	40	theme	well-resolved	970:982	arg1	transitions					984:994	well-resolved transitions	970:994	well-resolved transitions that can be used as a fingerprint to identify a particular glycan	970:1060	The recorded vibrational spectra exhibit well-resolved transitions that can be used as a fingerprint to identify a particular glycan.					
32749397	4	41	theme	ultrahigh-resolution	644:663	arg1	IMS					692:694	IMS	692:694	IMS	692:694	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	4	41	theme	ultrahigh-resolution	644:663	arg1	spectrometry					678:689	ultrahigh-resolution ion mobility spectrometry	644:689	ultrahigh-resolution ion mobility spectrometry (IMS)	644:695	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	4	42	theme	etanercept	848:857	arg1	range					864:868	the therapeutic fusion protein etanercept that range	817:868	the therapeutic fusion protein etanercept that range in abundance	817:881	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	2	43	theme	quality	341:347	arg1	glycosylation					298:310	glycosylation	298:310	glycosylation	298:310	As a result, glycosylation is categorized as a critical quality attribute that needs an adequate analytical approach to guarantee product quality.					
32749397	2	43	theme	quality	341:347	arg1	attribute					349:357	a critical quality attribute	330:357	a critical quality attribute that needs an adequate analytical approach to guarantee product quality	330:429	As a result, glycosylation is categorized as a critical quality attribute that needs an adequate analytical approach to guarantee product quality.					
32749397	0	44	theme	ion	83:85	arg1	spectrometry					96:107	ultrahigh-resolution ion mobility spectrometry	62:107	ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy	62:148	Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy.					
32749397	5	45	theme	particular	1044:1053	arg1	glycan					1055:1060	a particular glycan	1042:1060	a particular glycan	1042:1060	The recorded vibrational spectra exhibit well-resolved transitions that can be used as a fingerprint to identify a particular glycan.					
32749397	1	46	from	impact	225:230	arg1	clearance					262:270	clearance	262:270	clearance	262:270	Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety.					
32749397	1	46	from	impact	225:230	arg1	safety					277:282	safety	277:282	safety	277:282	Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety.					
32749397	1	46	from	impact	225:230	arg1	activity					252:259	their biological activity	235:259	their biological activity	235:259	Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety.					
32749397	6	47	theme	biopharmaceutical	1154:1170	arg1	tool					1214:1217	tool	1214:1217	tool for monitoring biopharmaceutical glycoforms	1214:1261	This work represents an important advance in the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms.					
32749397	6	47	theme	biopharmaceutical	1154:1170	arg1	proteins					1172:1179	biopharmaceutical proteins	1154:1179	biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms	1154:1261	This work represents an important advance in the analysis of N-linked glycans cleaved from biopharmaceutical proteins that could eventually be used as tool for monitoring biopharmaceutical glycoforms.					
32749397	3	48	theme	branched	469:476	arg1	structure					478:486	branched structure	469:486	branched structure	469:486	However, the isomeric complexity and branched structure of glycans makes their analysis a significant challenge.					
32749397	4	49	theme	mobility	669:676	arg1	IMS					692:694	IMS	692:694	IMS	692:694	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	4	49	theme	mobility	669:676	arg1	spectrometry					678:689	ultrahigh-resolution ion mobility spectrometry	644:689	ultrahigh-resolution ion mobility spectrometry (IMS)	644:695	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	0	50	theme	ultrahigh-resolution	62:81	arg1	spectrometry					96:107	ultrahigh-resolution ion mobility spectrometry	62:107	ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy	62:148	Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy.					
32749397	3	51	dep	makes	499:503	arg1	analysis					511:518	their analysis	505:518	their analysis	505:518	However, the isomeric complexity and branched structure of glycans makes their analysis a significant challenge.					
32749397	2	52	theme	analytical	382:391	arg1	approach					393:400	an adequate analytical approach	370:400	an adequate analytical approach to guarantee product quality	370:429	As a result, glycosylation is categorized as a critical quality attribute that needs an adequate analytical approach to guarantee product quality.					
32749397	4	53	theme	ion	665:667	arg1	IMS					692:694	IMS	692:694	IMS	692:694	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
32749397	4	53	theme	ion	665:667	arg1	spectrometry					678:689	ultrahigh-resolution ion mobility spectrometry	644:689	ultrahigh-resolution ion mobility spectrometry (IMS)	644:695	In this work, we propose a multidimensional approach for monitoring released glycans that combines ultrahigh-resolution ion mobility spectrometry (IMS) and cryogenic vibrational spectroscopy, and we demonstrate this technique by characterizing four N-glycans cleaved from the therapeutic fusion protein etanercept that range in abundance from 1% to 22% of the total N-glycan content.					
34231555	0	0	theme	monoclonal	74:83	arg1	antibodies					85:94	monoclonal antibodies	74:94	monoclonal antibodies	74:94	A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies.					
34231555	2	1	theme	terminal	348:355	arg1	position					367:374	the terminal galactose position	344:374	the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm	344:421	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	2	2	theme	effector	445:452	arg1	functions					454:462	effector functions	445:462	effector functions	445:462	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	3	3	theme	mAb	605:607	arg1	quality					609:615	guarantee mAb quality	595:615	guarantee mAb quality	595:615	The development of a robust method to distinguish positional isomers of glycans is thus critical to guarantee mAb quality.					
34231555	6	4	theme	host	1040:1043	arg1	line					1050:1053	host cell line	1040:1053	the host cell line (CHO and HEK-293)	1036:1071	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	5	5	theme	infrared	944:951	arg1	spectra					953:959	the infrared spectra	940:959	the infrared spectra	940:959	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	5	6	theme	isomeric	981:988	arg1	forms					990:994	their respective isomeric forms	964:994	their respective isomeric forms	964:994	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	3	7	theme	method	523:528	arg1	critical					583:590	critical	583:590	critical	583:590	The development of a robust method to distinguish positional isomers of glycans is thus critical to guarantee mAb quality.					
34231555	3	7	theme	method	523:528	arg1	development					499:509	The development	495:509	The development of a robust method to distinguish positional isomers of glycans	495:573	The development of a robust method to distinguish positional isomers of glycans is thus critical to guarantee mAb quality.					
34231555	2	8	theme	mAbs	489:492	arg1	functions					454:462	effector functions	445:462	effector functions	445:462	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	2	8	theme	mAbs	489:492	arg1	structure					476:484	dynamic structure	468:484	dynamic structure	468:484	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	2	9	from	impact	431:436	arg1	functions					454:462	effector functions	445:462	effector functions	445:462	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	2	9	from	impact	431:436	arg1	structure					476:484	dynamic structure	468:484	dynamic structure	468:484	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	3	10	theme	robust	516:521	arg1	method					523:528	a robust method	514:528	a robust method to distinguish positional isomers of glycans	514:573	The development of a robust method to distinguish positional isomers of glycans is thus critical to guarantee mAb quality.					
34231555	4	11	theme	ion	657:659	arg1	mobility					661:668	high-resolution ion mobility	641:668	high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions	641:790	In this work, we apply high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions, using G1F as an example.					
34231555	7	12	theme	glycan	1179:1184	arg1	analysis					1186:1193	glycan analysis	1179:1193	glycan analysis of mAbs	1179:1201	This work illustrates the potential of our approach for glycan analysis of mAbs.					
34231555	4	13	theme	high-resolution	641:655	arg1	mobility					661:668	high-resolution ion mobility	641:668	high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions	641:790	In this work, we apply high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions, using G1F as an example.					
34231555	2	14	contain	have	423:426	arg1	isomers					331:337	glycan isomers	324:337	glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm	324:421	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	2	14	contain	have	423:426	arg2	impact					431:436	an impact	428:436	an impact on the effector functions and dynamic structure of mAbs	428:492	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	4	15	theme	different	753:761	arg1	positions					782:790	different terminal galactose positions	753:790	different terminal galactose positions	753:790	In this work, we apply high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions, using G1F as an example.					
34231555	2	16	theme	Man	390:392	arg1	arm					399:401	the Man α1-3 arm	386:401	the Man α1-3 arm	386:401	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	5	17	theme	F	863:863	arg1	isomer					865:870	the G1(α1-6)F isomer	851:870	the G1(α1-6)F isomer	851:870	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	6	18	theme	G1F	1084:1086	arg1	profile					1094:1100	the IgG G1F gycan profile	1076:1100	the IgG G1F gycan profile at the isomer level	1076:1120	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	0	19	theme	new	2:4	arg1	approach					6:13	A new approach	0:13	A new approach for identifying positional isomers of glycans	0:59	A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies.					
34231555	6	20	dep	line	1050:1053	arg1	CHO					1056:1058	CHO	1056:1058	CHO	1056:1058	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	6	20	dep	line	1050:1053	arg1	HEK-293					1064:1070	HEK-293	1064:1070	HEK-293	1064:1070	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	6	21	theme	IgG	1080:1082	arg1	profile					1094:1100	the IgG G1F gycan profile	1076:1100	the IgG G1F gycan profile at the isomer level	1076:1120	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	1	22	theme	different	183:191	arg1	types					203:207	different host cell types	183:207	different host cell types	183:207	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	2	23	from	position	367:374	arg1	arm					419:421	the Man α1-6 arm	406:421	the Man α1-6 arm	406:421	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	2	23	from	position	367:374	arg1	arm					399:401	the Man α1-3 arm	386:401	the Man α1-3 arm	386:401	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	4	24	theme	infrared	694:701	arg1	spectroscopy					703:714	cryogenic infrared spectroscopy	684:714	cryogenic infrared spectroscopy	684:714	In this work, we apply high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions, using G1F as an example.					
34231555	1	25	theme	host	193:196	arg1	types					203:207	different host cell types	183:207	different host cell types	183:207	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	2	26	theme	α1-6	414:417	arg1	arm					419:421	the Man α1-6 arm	406:421	the Man α1-6 arm	406:421	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	6	27	from	impact	1026:1031	arg1	profile					1094:1100	the IgG G1F gycan profile	1076:1100	the IgG G1F gycan profile at the isomer level	1076:1120	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	1	28	theme	Glycosylation	97:109	arg1	patterns					111:118	Glycosylation patterns	97:118	Glycosylation patterns in monoclonal antibodies (mAbs)	97:150	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	1	29	theme	cell	198:201	arg1	types					203:207	different host cell types	183:207	different host cell types	183:207	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	2	30	theme	Man	410:412	arg1	arm					419:421	the Man α1-6 arm	406:421	the Man α1-6 arm	406:421	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	4	31	with	glycans	740:746	arg1	positions					782:790	different terminal galactose positions	753:790	different terminal galactose positions	753:790	In this work, we apply high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions, using G1F as an example.					
34231555	6	32	theme	gycan	1088:1092	arg1	profile					1094:1100	the IgG G1F gycan profile	1076:1100	the IgG G1F gycan profile at the isomer level	1076:1120	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	4	33	theme	isomeric	731:738	arg1	glycans					740:746	isomeric glycans	731:746	isomeric glycans with different terminal galactose positions	731:790	In this work, we apply high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions, using G1F as an example.					
34231555	2	34	theme	Recent	286:291	arg1	studies					293:299	Recent studies	286:299	Recent studies	286:299	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	5	35	theme	arrival-time	909:920	arg1	distributions					922:934	the arrival-time distributions	905:934	the arrival-time distributions	905:934	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	0	36	theme	positional	31:40	arg1	isomers					42:48	positional isomers	31:48	positional isomers of glycans	31:59	A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies.					
34231555	7	37	theme	mAbs	1198:1201	arg1	analysis					1186:1193	glycan analysis	1179:1193	glycan analysis of mAbs	1179:1201	This work illustrates the potential of our approach for glycan analysis of mAbs.					
34231555	5	38	theme	G1	855:856	arg1	isomer					865:870	the G1(α1-6)F isomer	851:870	the G1(α1-6)F isomer	851:870	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	5	39	theme	Selective	818:826	arg1	synthesis					838:846	Selective enzymatic synthesis	818:846	Selective enzymatic synthesis of the G1(α1-6)F isomer	818:870	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	3	40	theme	guarantee	595:603	arg1	quality					609:615	guarantee mAb quality	595:615	guarantee mAb quality	595:615	The development of a robust method to distinguish positional isomers of glycans is thus critical to guarantee mAb quality.					
34231555	3	41	theme	glycans	567:573	arg1	isomers					556:562	positional isomers	545:562	positional isomers of glycans	545:573	The development of a robust method to distinguish positional isomers of glycans is thus critical to guarantee mAb quality.					
34231555	2	42	theme	α1-3	394:397	arg1	arm					399:401	the Man α1-3 arm	386:401	the Man α1-3 arm	386:401	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	2	43	dep	functions	454:462	arg1	the					441:443	the	441:443	the	441:443	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	2	44	with	isomers	331:337	arg1	position					367:374	the terminal galactose position	344:374	the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm	344:421	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	3	45	theme	positional	545:554	arg1	isomers					556:562	positional isomers	545:562	positional isomers of glycans	545:573	The development of a robust method to distinguish positional isomers of glycans is thus critical to guarantee mAb quality.					
34231555	5	46	theme	isomer	865:870	arg1	synthesis					838:846	Selective enzymatic synthesis	818:846	Selective enzymatic synthesis of the G1(α1-6)F isomer	818:870	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	1	47	theme	monoclonal	123:132	arg1	antibodies					134:143	monoclonal antibodies	123:143	monoclonal antibodies (mAbs)	123:150	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	1	47	theme	monoclonal	123:132	arg1	mAbs					146:149	mAbs	146:149	mAbs	146:149	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	5	48	from	peaks	896:900	arg1	spectra					953:959	the infrared spectra	940:959	the infrared spectra	940:959	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	5	48	from	peaks	896:900	arg1	distributions					922:934	the arrival-time distributions	905:934	the arrival-time distributions	905:934	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	7	49	theme	approach	1166:1173	arg1	potential					1149:1157	the potential	1145:1157	the potential of our approach for glycan analysis of mAbs	1145:1201	This work illustrates the potential of our approach for glycan analysis of mAbs.					
34231555	4	50	theme	galactose	772:780	arg1	positions					782:790	different terminal galactose positions	753:790	different terminal galactose positions	753:790	In this work, we apply high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions, using G1F as an example.					
34231555	5	51	theme	respective	970:979	arg1	forms					990:994	their respective isomeric forms	964:994	their respective isomeric forms	964:994	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	2	52	theme	glycan	324:329	arg1	isomers					331:337	glycan isomers	324:337	glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm	324:421	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	1	53	from	patterns	111:118	arg1	antibodies					134:143	monoclonal antibodies	123:143	monoclonal antibodies (mAbs)	123:150	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	1	53	from	patterns	111:118	arg1	mAbs					146:149	mAbs	146:149	mAbs	146:149	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	1	54	dep	mAbs	243:246	arg1	immunogenicity					270:283	immunogenicity	270:283	immunogenicity	270:283	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	1	54	dep	mAbs	243:246	arg1	safety					248:253	safety	248:253	safety	248:253	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	1	54	dep	mAbs	243:246	arg1	mAbs					243:246	mAbs safety, efficacy, and immunogenicity	243:283	mAbs safety, efficacy, and immunogenicity	243:283	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	1	54	dep	mAbs	243:246	arg1	efficacy					256:263	efficacy	256:263	efficacy	256:263	Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity.					
34231555	4	55	theme	terminal	763:770	arg1	positions					782:790	different terminal galactose positions	753:790	different terminal galactose positions	753:790	In this work, we apply high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions, using G1F as an example.					
34231555	6	56	theme	cell	1045:1048	arg1	line					1050:1053	host cell line	1040:1053	the host cell line (CHO and HEK-293)	1036:1071	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	4	57	theme	cryogenic	684:692	arg1	spectroscopy					703:714	cryogenic infrared spectroscopy	684:714	cryogenic infrared spectroscopy	684:714	In this work, we apply high-resolution ion mobility combined with cryogenic infrared spectroscopy to distinguish isomeric glycans with different terminal galactose positions, using G1F as an example.					
34231555	2	58	theme	dynamic	468:474	arg1	structure					476:484	dynamic structure	468:484	dynamic structure	468:484	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
34231555	5	59	theme	enzymatic	828:836	arg1	synthesis					838:846	Selective enzymatic synthesis	818:846	Selective enzymatic synthesis of the G1(α1-6)F isomer	818:870	Selective enzymatic synthesis of the G1(α1-6)F isomer allows us to assign the peaks in the arrival-time distributions and the infrared spectra to their respective isomeric forms.					
34231555	6	60	theme	isomer	1109:1114	arg1	level					1116:1120	the isomer level	1105:1120	the isomer level	1105:1120	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	6	61	theme	line	1050:1053	arg1	impact					1026:1031	the impact	1022:1031	the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level	1022:1120	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	6	62	from	level	1116:1120	arg1	profile					1094:1100	the IgG G1F gycan profile	1076:1100	the IgG G1F gycan profile at the isomer level	1076:1120	Moreover, we demonstrate the impact of the host cell line (CHO and HEK-293) on the IgG G1F gycan profile at the isomer level.					
34231555	0	63	theme	glycans	53:59	arg1	isomers					42:48	positional isomers	31:48	positional isomers of glycans	31:59	A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies.					
34231555	2	64	theme	galactose	357:365	arg1	position					367:374	the terminal galactose position	344:374	the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm	344:421	Recent studies have demonstrated that glycan isomers with the terminal galactose position on either the Man α1-3 arm or the Man α1-6 arm have an impact on the effector functions and dynamic structure of mAbs.					
33766590	0	0	theme	enzyme-assisted	92:106	arg1	extraction					108:117	enzyme-assisted extraction	92:117	enzyme-assisted extraction	92:117	Anti-glycation effect of Ecklonia cava polysaccharides extracted by combined ultrasound and enzyme-assisted extraction.					
33766590	1	1	theme	enzyme	312:317	arg1	combination					282:292	a combination	280:292	a combination of ultrasound and enzyme (UEP)	280:323	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	1	theme	enzyme	312:317	arg1	ultrasound					247:256	ultrasound	247:256	ultrasound (UP)	247:261	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	1	theme	enzyme	312:317	arg1	EP					272:273	EP	272:273	EP	272:273	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	1	theme	enzyme	312:317	arg1	UP					259:260	UP	259:260	UP	259:260	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	1	theme	enzyme	312:317	arg1	buffer					234:239	extraction method-hot buffer	212:239	extraction method-hot buffer (HP)	212:244	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	1	theme	enzyme	312:317	arg1	HP					242:243	HP	242:243	HP	242:243	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	1	theme	enzyme	312:317	arg1	enzyme					264:269	enzyme	264:269	enzyme (EP)	264:274	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	5	2	theme	1H	620:621	arg1	NMR					623:625	1H NMR	620:625	1H NMR	620:625	1H NMR indicated that alginate was removed by purification.					
33766590	10	3	theme	protein	1187:1193	arg1	carbonyl					1195:1202	protein carbonyl	1187:1202	protein carbonyl	1187:1202	However, UP and UEP showed higher inhibition of fructosamine, α-dicarbonyl, and protein carbonyl than aminoguanidine.					
33766590	4	4	theme	samples	549:555	arg1	spectra					538:544	The FT-IR spectra	528:544	The FT-IR spectra of samples	528:555	The FT-IR spectra of samples showed the presence of sulfate esters and 4-sulfate galactose.					
33766590	3	5	contain	had	474:476	arg1	EP					462:463	EP	462:463	EP	462:463	UP, EP, and UEP had higher fucose and galactose compositions than HP.					
33766590	3	5	contain	had	474:476	arg1	UEP					470:472	UEP	470:472	UEP	470:472	UP, EP, and UEP had higher fucose and galactose compositions than HP.					
33766590	3	5	contain	had	474:476	arg1	UP					458:459	UP	458:459	UP	458:459	UP, EP, and UEP had higher fucose and galactose compositions than HP.					
33766590	3	5	contain	had	474:476	arg2	compositions					506:517	higher fucose and galactose compositions	478:517	higher fucose and galactose compositions	478:517	UP, EP, and UEP had higher fucose and galactose compositions than HP.					
33766590	11	6	theme	AGE	1225:1227	arg1	formation					1229:1237	AGE formation	1225:1237	AGE formation	1225:1237	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	8	7	theme	carbonyl	912:919	arg1	contents					921:928	protein carbonyl contents	904:928	protein carbonyl contents	904:928	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system.					
33766590	3	8	theme	higher	478:483	arg1	compositions					506:517	higher fucose and galactose compositions	478:517	higher fucose and galactose compositions	478:517	UP, EP, and UEP had higher fucose and galactose compositions than HP.					
33766590	10	9	theme	higher	1134:1139	arg1	inhibition					1141:1150	higher inhibition	1134:1150	higher inhibition of fructosamine, α-dicarbonyl, and protein carbonyl	1134:1202	However, UP and UEP showed higher inhibition of fructosamine, α-dicarbonyl, and protein carbonyl than aminoguanidine.					
33766590	9	10	theme	inhibition	1050:1059	arg1	lower					1072:1076	lower	1072:1076	lower	1072:1076	UEP and UP effectively inhibited AGE, although the inhibition effect was lower than that of aminoguanidine.					
33766590	9	10	theme	inhibition	1050:1059	arg1	effect					1061:1066	the inhibition effect	1046:1066	the inhibition effect	1046:1066	UEP and UP effectively inhibited AGE, although the inhibition effect was lower than that of aminoguanidine.					
33766590	8	11	theme	BSA/fructose	978:989	arg1	system					991:996	a BSA/fructose system	976:996	a BSA/fructose system	976:996	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system.					
33766590	8	12	theme	protein	904:910	arg1	contents					921:928	protein carbonyl contents	904:928	protein carbonyl contents	904:928	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system.					
33766590	1	13	from	Ecklonia	171:178	arg1	polysaccharides					150:164	polysaccharides	150:164	polysaccharides from Ecklonia cava	150:183	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	13	from	Ecklonia	171:178	arg1	effects					139:145	The anti-glycation effects	120:145	The anti-glycation effects of polysaccharides from Ecklonia cava	120:183	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	11	14	theme	molecular	1388:1396	arg1	weight					1398:1403	molecular weight	1388:1403	molecular weight	1388:1403	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	7	15	theme	uronic	810:815	arg1	contents					822:829	protein and uronic acid contents	798:829	contents	822:829	UEP presented with the highest extraction yield and lowest protein and uronic acid contents.					
33766590	12	16	theme	extraction	1465:1474	arg1	method					1476:1481	a suitable extraction method	1454:1481	a suitable extraction method for obtaining anti-glycation agents from E. cava	1454:1530	Overall, the present study suggests that UEP is a suitable extraction method for obtaining anti-glycation agents from E. cava.					
33766590	12	16	theme	extraction	1465:1474	arg1	UEP					1447:1449	UEP	1447:1449	UEP	1447:1449	Overall, the present study suggests that UEP is a suitable extraction method for obtaining anti-glycation agents from E. cava.					
33766590	8	17	theme	contents	921:928	arg1	levels					836:841	The levels	832:841	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents	832:928	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system.					
33766590	3	18	theme	galactose	496:504	arg1	compositions					506:517	higher fucose and galactose compositions	478:517	higher fucose and galactose compositions	478:517	UP, EP, and UEP had higher fucose and galactose compositions than HP.					
33766590	7	19	theme	acid	817:820	arg1	contents					822:829	protein and uronic acid contents	798:829	contents	822:829	UEP presented with the highest extraction yield and lowest protein and uronic acid contents.					
33766590	12	20	theme	suitable	1456:1463	arg1	method					1476:1481	a suitable extraction method	1454:1481	a suitable extraction method for obtaining anti-glycation agents from E. cava	1454:1530	Overall, the present study suggests that UEP is a suitable extraction method for obtaining anti-glycation agents from E. cava.					
33766590	12	20	theme	suitable	1456:1463	arg1	UEP					1447:1449	UEP	1447:1449	UEP	1447:1449	Overall, the present study suggests that UEP is a suitable extraction method for obtaining anti-glycation agents from E. cava.					
33766590	0	21	theme	Anti-glycation	0:13	arg1	effect					15:20	Anti-glycation effect	0:20	Anti-glycation effect of Ecklonia cava polysaccharides	0:53	Anti-glycation effect of Ecklonia cava polysaccharides extracted by combined ultrasound and enzyme-assisted extraction.					
33766590	7	22	theme	protein	798:804	arg1	contents					822:829	protein and uronic acid contents	798:829	contents	822:829	UEP presented with the highest extraction yield and lowest protein and uronic acid contents.					
33766590	2	23	theme	monosaccharide	358:371	arg1	compositions					373:384	monosaccharide compositions	358:384	monosaccharide compositions	358:384	The physicochemical properties, monosaccharide compositions, and structural characteristics of the polysaccharides were determined.					
33766590	6	24	contain	possessed	696:704	arg2	contents					721:728	higher sulfate contents	706:728	higher sulfate contents	706:728	UP, EP, and UEP possessed higher sulfate contents than HP.					
33766590	6	24	contain	possessed	696:704	arg1	EP					684:685	EP	684:685	EP	684:685	UP, EP, and UEP possessed higher sulfate contents than HP.					
33766590	6	24	contain	possessed	696:704	arg1	UEP					692:694	UEP	692:694	UEP	692:694	UP, EP, and UEP possessed higher sulfate contents than HP.					
33766590	6	24	contain	possessed	696:704	arg1	UP					680:681	UP	680:681	UP	680:681	UP, EP, and UEP possessed higher sulfate contents than HP.					
33766590	8	25	from	incubation	962:971	arg1	system					991:996	a BSA/fructose system	976:996	a BSA/fructose system	976:996	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system.					
33766590	8	26	theme	3-week	955:960	arg1	incubation					962:971	a 3-week incubation	953:971	a 3-week incubation in a BSA/fructose system	953:996	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system.					
33766590	11	27	dep	compositions	1310:1321	arg1	galactose					1332:1340	galactose	1332:1340	galactose	1332:1340	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	11	27	dep	compositions	1310:1321	arg1	glucose					1347:1353	glucose	1347:1353	glucose	1347:1353	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	11	27	dep	compositions	1310:1321	arg1	fucose					1324:1329	fucose	1324:1329	fucose	1324:1329	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	11	27	dep	compositions	1310:1321	arg1	compositions					1310:1321	some monosaccharide compositions	1290:1321	some monosaccharide compositions (fucose, galactose, and glucose)	1290:1354	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	1	28	theme	extraction	212:221	arg1	HP					242:243	HP	242:243	HP	242:243	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	28	theme	extraction	212:221	arg1	buffer					234:239	extraction method-hot buffer	212:239	extraction method-hot buffer (HP)	212:244	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	2	29	theme	polysaccharides	425:439	arg1	properties					346:355	The physicochemical properties	326:355	The physicochemical properties	326:355	The physicochemical properties, monosaccharide compositions, and structural characteristics of the polysaccharides were determined.					
33766590	2	29	theme	polysaccharides	425:439	arg1	compositions					373:384	monosaccharide compositions	358:384	monosaccharide compositions	358:384	The physicochemical properties, monosaccharide compositions, and structural characteristics of the polysaccharides were determined.					
33766590	2	29	theme	polysaccharides	425:439	arg1	characteristics					402:416	structural characteristics	391:416	structural characteristics	391:416	The physicochemical properties, monosaccharide compositions, and structural characteristics of the polysaccharides were determined.					
33766590	1	30	from	effects	139:145	arg1	Ecklonia					171:178	Ecklonia	171:178	Ecklonia	171:178	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	8	31	theme	AGE	846:848	arg1	formation					850:858	AGE formation	846:858	AGE formation	846:858	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system.					
33766590	1	32	theme	method-hot	223:232	arg1	HP					242:243	HP	242:243	HP	242:243	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	32	theme	method-hot	223:232	arg1	buffer					234:239	extraction method-hot buffer	212:239	extraction method-hot buffer (HP)	212:244	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	0	33	theme	Ecklonia	25:32	arg1	polysaccharides					39:53	Ecklonia cava polysaccharides	25:53	Ecklonia cava polysaccharides	25:53	Anti-glycation effect of Ecklonia cava polysaccharides extracted by combined ultrasound and enzyme-assisted extraction.					
33766590	7	34	theme	highest	762:768	arg1	yield					781:785	the highest extraction yield	758:785	the highest extraction yield	758:785	UEP presented with the highest extraction yield and lowest protein and uronic acid contents.					
33766590	1	35	dep	Ecklonia	171:178	arg1	cava					180:183	Ecklonia cava	171:183	Ecklonia cava	171:183	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	4	36	theme	4-sulfate	599:607	arg1	galactose					609:617	4-sulfate galactose	599:617	4-sulfate galactose	599:617	The FT-IR spectra of samples showed the presence of sulfate esters and 4-sulfate galactose.					
33766590	11	37	theme	sulfate	1270:1276	arg1	content					1278:1284	sulfate content	1270:1284	sulfate content	1270:1284	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	2	38	theme	physicochemical	330:344	arg1	properties					346:355	The physicochemical properties	326:355	The physicochemical properties	326:355	The physicochemical properties, monosaccharide compositions, and structural characteristics of the polysaccharides were determined.					
33766590	1	39	theme	anti-glycation	124:137	arg1	effects					139:145	The anti-glycation effects	120:145	The anti-glycation effects of polysaccharides from Ecklonia cava	120:183	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	7	40	theme	extraction	770:779	arg1	yield					781:785	the highest extraction yield	758:785	the highest extraction yield	758:785	UEP presented with the highest extraction yield and lowest protein and uronic acid contents.					
33766590	2	41	theme	structural	391:400	arg1	characteristics					402:416	structural characteristics	391:416	structural characteristics	391:416	The physicochemical properties, monosaccharide compositions, and structural characteristics of the polysaccharides were determined.					
33766590	0	42	theme	polysaccharides	39:53	arg1	effect					15:20	Anti-glycation effect	0:20	Anti-glycation effect of Ecklonia cava polysaccharides	0:53	Anti-glycation effect of Ecklonia cava polysaccharides extracted by combined ultrasound and enzyme-assisted extraction.					
33766590	4	43	theme	FT-IR	532:536	arg1	spectra					538:544	The FT-IR spectra	528:544	The FT-IR spectra of samples	528:555	The FT-IR spectra of samples showed the presence of sulfate esters and 4-sulfate galactose.					
33766590	8	44	theme	formation	850:858	arg1	levels					836:841	The levels	832:841	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents	832:928	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system.					
33766590	0	45	theme	cava	34:37	arg1	polysaccharides					39:53	Ecklonia cava polysaccharides	25:53	Ecklonia cava polysaccharides	25:53	Anti-glycation effect of Ecklonia cava polysaccharides extracted by combined ultrasound and enzyme-assisted extraction.					
33766590	4	46	theme	galactose	609:617	arg1	presence					568:575	the presence	564:575	the presence of sulfate esters and 4-sulfate galactose	564:617	The FT-IR spectra of samples showed the presence of sulfate esters and 4-sulfate galactose.					
33766590	6	47	theme	sulfate	713:719	arg1	contents					721:728	higher sulfate contents	706:728	higher sulfate contents	706:728	UP, EP, and UEP possessed higher sulfate contents than HP.					
33766590	11	48	theme	monosaccharide	1295:1308	arg1	galactose					1332:1340	galactose	1332:1340	galactose	1332:1340	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	11	48	theme	monosaccharide	1295:1308	arg1	glucose					1347:1353	glucose	1347:1353	glucose	1347:1353	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	11	48	theme	monosaccharide	1295:1308	arg1	fucose					1324:1329	fucose	1324:1329	fucose	1324:1329	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	11	48	theme	monosaccharide	1295:1308	arg1	compositions					1310:1321	some monosaccharide compositions	1290:1321	some monosaccharide compositions (fucose, galactose, and glucose)	1290:1354	AGE formation was negatively correlated with sulfate content and some monosaccharide compositions (fucose, galactose, and glucose), but positively correlated with molecular weight.					
33766590	6	49	theme	higher	706:711	arg1	contents					721:728	higher sulfate contents	706:728	higher sulfate contents	706:728	UP, EP, and UEP possessed higher sulfate contents than HP.					
33766590	10	50	theme	α-dicarbonyl	1169:1180	arg1	inhibition					1141:1150	higher inhibition	1134:1150	higher inhibition of fructosamine, α-dicarbonyl, and protein carbonyl	1134:1202	However, UP and UEP showed higher inhibition of fructosamine, α-dicarbonyl, and protein carbonyl than aminoguanidine.					
33766590	1	51	theme	ultrasound	297:306	arg1	combination					282:292	a combination	280:292	a combination of ultrasound and enzyme (UEP)	280:323	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	51	theme	ultrasound	297:306	arg1	ultrasound					247:256	ultrasound	247:256	ultrasound (UP)	247:261	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	51	theme	ultrasound	297:306	arg1	EP					272:273	EP	272:273	EP	272:273	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	51	theme	ultrasound	297:306	arg1	UP					259:260	UP	259:260	UP	259:260	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	51	theme	ultrasound	297:306	arg1	buffer					234:239	extraction method-hot buffer	212:239	extraction method-hot buffer (HP)	212:244	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	51	theme	ultrasound	297:306	arg1	HP					242:243	HP	242:243	HP	242:243	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	51	theme	ultrasound	297:306	arg1	enzyme					264:269	enzyme	264:269	enzyme (EP)	264:274	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	1	52	theme	polysaccharides	150:164	arg1	effects					139:145	The anti-glycation effects	120:145	The anti-glycation effects of polysaccharides from Ecklonia cava	120:183	The anti-glycation effects of polysaccharides from Ecklonia cava were examined according to extraction method-hot buffer (HP), ultrasound (UP), enzyme (EP), or a combination of ultrasound and enzyme (UEP).					
33766590	8	53	theme	α-dicarbonyl	886:897	arg1	levels					836:841	The levels	832:841	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents	832:928	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system.					
33766590	10	54	theme	fructosamine	1155:1166	arg1	inhibition					1141:1150	higher inhibition	1134:1150	higher inhibition of fructosamine, α-dicarbonyl, and protein carbonyl	1134:1202	However, UP and UEP showed higher inhibition of fructosamine, α-dicarbonyl, and protein carbonyl than aminoguanidine.					
33766590	12	55	theme	present	1419:1425	arg1	study					1427:1431	the present study	1415:1431	the present study	1415:1431	Overall, the present study suggests that UEP is a suitable extraction method for obtaining anti-glycation agents from E. cava.					
33766590	7	56	dep	yield	781:785	arg1	contents					822:829	protein and uronic acid contents	798:829	contents	822:829	UEP presented with the highest extraction yield and lowest protein and uronic acid contents.					
33766590	3	57	theme	fucose	485:490	arg1	compositions					506:517	higher fucose and galactose compositions	478:517	higher fucose and galactose compositions	478:517	UP, EP, and UEP had higher fucose and galactose compositions than HP.					
33766590	4	58	theme	esters	588:593	arg1	presence					568:575	the presence	564:575	the presence of sulfate esters and 4-sulfate galactose	564:617	The FT-IR spectra of samples showed the presence of sulfate esters and 4-sulfate galactose.					
33766590	0	59	theme	combined	68:75	arg1	ultrasound					77:86	combined ultrasound	68:86	combined ultrasound	68:86	Anti-glycation effect of Ecklonia cava polysaccharides extracted by combined ultrasound and enzyme-assisted extraction.					
33766590	12	60	theme	anti-glycation	1497:1510	arg1	agents					1512:1517	anti-glycation agents	1497:1517	anti-glycation agents	1497:1517	Overall, the present study suggests that UEP is a suitable extraction method for obtaining anti-glycation agents from E. cava.					
33766590	8	61	theme	fructosamine	872:883	arg1	levels					836:841	The levels	832:841	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents	832:928	The levels of AGE formation, as well as fructosamine, α-dicarbonyl, and protein carbonyl contents were determined during a 3-week incubation in a BSA/fructose system.					
33766590	10	62	theme	carbonyl	1195:1202	arg1	inhibition					1141:1150	higher inhibition	1134:1150	higher inhibition of fructosamine, α-dicarbonyl, and protein carbonyl	1134:1202	However, UP and UEP showed higher inhibition of fructosamine, α-dicarbonyl, and protein carbonyl than aminoguanidine.					
33766590	4	63	theme	sulfate	580:586	arg1	esters					588:593	sulfate esters	580:593	sulfate esters	580:593	The FT-IR spectra of samples showed the presence of sulfate esters and 4-sulfate galactose.					
32008322	0	0	theme	Fc-glycan	90:98	arg1	Structure					100:108	the Fc-glycan Structure	86:108	the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761	86:151	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	5	1	theme	loss	1243:1246	arg1	degree					1213:1218	the degree	1209:1218	the degree of Fc-glycan structure loss	1209:1246	Measurements revealed decreases in structural stability correlating with the degree of Fc-glycan structure loss, especially at the CH2/CH3 domain interface.					
32008322	2	2	theme	procedures	525:534	arg1	suitability					454:464	suitability	454:464	suitability	454:464	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	2	2	theme	procedures	525:534	arg1	robustness					470:479	robustness	470:479	robustness	470:479	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	3	3	theme	spectrometry-mass	866:882	arg1	IMS-MS					898:903	IMS-MS	898:903	IMS-MS	898:903	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	3	3	theme	spectrometry-mass	866:882	arg1	spectrometry					884:895	ion mobility spectrometry-mass spectrometry	853:895	ion mobility spectrometry-mass spectrometry (IMS-MS)	853:904	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	3	4	theme	deuterium	803:811	arg1	HDX-MS					841:846	HDX-MS	841:846	HDX-MS	841:846	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	3	4	theme	deuterium	803:811	arg1	spectrometry					827:838	hydrogen deuterium exchange-mass spectrometry	794:838	hydrogen deuterium exchange-mass spectrometry (HDX-MS)	794:847	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	2	5	theme	analytical	514:523	arg1	procedures					525:534	both established and emerging analytical procedures	484:534	both established and emerging analytical procedures	484:534	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	1	6	theme	protein	318:324	arg1	structure					339:347	protein higher order structure	318:347	protein higher order structure (HOS) of these biomolecules	318:375	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	1	6	theme	protein	318:324	arg1	HOS					350:352	HOS	350:352	HOS	350:352	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	6	7	theme	RM	1328:1329	arg1	use					1316:1318	the use	1312:1318	the use of this RM and these Fc-glycan variants for establishing the sensitivity of and validating analytical methods for the detection of HOS measurements of mAbs	1312:1474	These data promote the use of this RM and these Fc-glycan variants for establishing the sensitivity of and validating analytical methods for the detection of HOS measurements of mAbs.					
32008322	3	8	dep	spectrometry	827:838	arg1	measurements					906:917	measurements	906:917	measurements	906:917	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	1	9	theme	monoclonal	157:166	arg1	mAbs					180:183	mAbs	180:183	mAbs	180:183	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	1	9	theme	monoclonal	157:166	arg1	antibodies					168:177	monoclonal antibodies	157:177	monoclonal antibodies (mAbs)	157:184	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	3	10	theme	exchange-mass	813:825	arg1	HDX-MS					841:846	HDX-MS	841:846	HDX-MS	841:846	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	3	10	theme	exchange-mass	813:825	arg1	spectrometry					827:838	hydrogen deuterium exchange-mass spectrometry	794:838	hydrogen deuterium exchange-mass spectrometry (HDX-MS)	794:847	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	2	11	theme	established	489:499	arg1	procedures					525:534	both established and emerging analytical procedures	484:534	both established and emerging analytical procedures	484:534	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	1	12	theme	higher	326:331	arg1	structure					339:347	protein higher order structure	318:347	protein higher order structure (HOS) of these biomolecules	318:375	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	1	12	theme	higher	326:331	arg1	HOS					350:352	HOS	350:352	HOS	350:352	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	6	13	theme	mAbs	1471:1474	arg1	measurements					1455:1466	HOS measurements	1451:1466	HOS measurements of mAbs	1451:1474	These data promote the use of this RM and these Fc-glycan variants for establishing the sensitivity of and validating analytical methods for the detection of HOS measurements of mAbs.					
32008322	3	14	theme	ion	853:855	arg1	IMS-MS					898:903	IMS-MS	898:903	IMS-MS	898:903	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	3	14	theme	ion	853:855	arg1	spectrometry					884:895	ion mobility spectrometry-mass spectrometry	853:895	ion mobility spectrometry-mass spectrometry (IMS-MS)	853:904	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	6	15	theme	analytical	1411:1420	arg1	methods					1422:1428	analytical methods	1411:1428	analytical methods for the detection of HOS measurements of mAbs	1411:1474	These data promote the use of this RM and these Fc-glycan variants for establishing the sensitivity of and validating analytical methods for the detection of HOS measurements of mAbs.					
32008322	4	16	theme	statistical	935:945	arg1	approach					947:954	An alternative statistical approach	920:954	An alternative statistical approach	920:954	An alternative statistical approach is described where measurement thresholds that differentiate between measurement variability and significant structural changes were established on the basis of experimental data.					
32008322	1	17	theme	order	333:337	arg1	structure					339:347	protein higher order structure	318:347	protein higher order structure (HOS) of these biomolecules	318:375	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	1	17	theme	order	333:337	arg1	HOS					350:352	HOS	350:352	HOS	350:352	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	3	18	theme	hydrogen	794:801	arg1	HDX-MS					841:846	HDX-MS	841:846	HDX-MS	841:846	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	3	18	theme	hydrogen	794:801	arg1	spectrometry					827:838	hydrogen deuterium exchange-mass spectrometry	794:838	hydrogen deuterium exchange-mass spectrometry (HDX-MS)	794:847	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	0	19	theme	NISTmAb	117:123	arg1	RM					145:146	RM 8761	145:151	RM 8761	145:151	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	0	19	theme	NISTmAb	117:123	arg1	Material					135:142	the NISTmAb Reference Material	113:142	the NISTmAb Reference Material	113:142	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	3	20	theme	localized	701:709	arg1	changes					711:717	both global and localized changes	685:717	both global and localized changes in HOS between the RM and these Fc-glycan variants	685:768	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	3	21	from	changes	711:717	arg1	HOS					722:724	HOS	722:724	HOS between the RM and these Fc-glycan variants	722:768	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	5	22	theme	CH2/CH3	1267:1273	arg1	interface					1282:1290	the CH2/CH3 domain interface	1263:1290	the CH2/CH3 domain interface	1263:1290	Measurements revealed decreases in structural stability correlating with the degree of Fc-glycan structure loss, especially at the CH2/CH3 domain interface.					
32008322	2	23	theme	key	411:413	arg1	purpose					426:432	a key analytical purpose	409:432	a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators	409:573	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	5	24	theme	structural	1171:1180	arg1	stability					1182:1190	structural stability	1171:1190	structural stability correlating with the degree of Fc-glycan structure loss	1171:1246	Measurements revealed decreases in structural stability correlating with the degree of Fc-glycan structure loss, especially at the CH2/CH3 domain interface.					
32008322	5	25	from	decreases	1158:1166	arg1	stability					1182:1190	structural stability	1171:1190	structural stability correlating with the degree of Fc-glycan structure loss	1171:1246	Measurements revealed decreases in structural stability correlating with the degree of Fc-glycan structure loss, especially at the CH2/CH3 domain interface.					
32008322	4	26	theme	alternative	923:933	arg1	approach					947:954	An alternative statistical approach	920:954	An alternative statistical approach	920:954	An alternative statistical approach is described where measurement thresholds that differentiate between measurement variability and significant structural changes were established on the basis of experimental data.					
32008322	0	27	theme	Spectrometry	5:16	arg1	Characterization					18:33	Mass Spectrometry Characterization	0:33	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.	0:152	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	3	28	theme	global	690:695	arg1	changes					711:717	both global and localized changes	685:717	both global and localized changes in HOS between the RM and these Fc-glycan variants	685:768	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	6	29	theme	variants	1351:1358	arg1	use					1316:1318	the use	1312:1318	the use of this RM and these Fc-glycan variants for establishing the sensitivity of and validating analytical methods for the detection of HOS measurements of mAbs	1312:1474	These data promote the use of this RM and these Fc-glycan variants for establishing the sensitivity of and validating analytical methods for the detection of HOS measurements of mAbs.					
32008322	0	30	theme	Mass	0:3	arg1	Characterization					18:33	Mass Spectrometry Characterization	0:33	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.	0:152	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	5	31	theme	Fc-glycan	1223:1231	arg1	loss					1243:1246	Fc-glycan structure loss	1223:1246	Fc-glycan structure loss	1223:1246	Measurements revealed decreases in structural stability correlating with the degree of Fc-glycan structure loss, especially at the CH2/CH3 domain interface.					
32008322	5	32	theme	structure	1233:1241	arg1	loss					1243:1246	Fc-glycan structure loss	1223:1246	Fc-glycan structure loss	1223:1246	Measurements revealed decreases in structural stability correlating with the degree of Fc-glycan structure loss, especially at the CH2/CH3 domain interface.					
32008322	0	33	theme	Material	135:142	arg1	Structure					100:108	the Fc-glycan Structure	86:108	the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761	86:151	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	4	34	theme	structural	1065:1074	arg1	changes					1076:1082	significant structural changes	1053:1082	significant structural changes	1053:1082	An alternative statistical approach is described where measurement thresholds that differentiate between measurement variability and significant structural changes were established on the basis of experimental data.					
32008322	1	35	theme	suitable	259:266	arg1	technologies					279:290	suitable analytical technologies	259:290	suitable analytical technologies	259:290	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	0	36	theme	Reference	125:133	arg1	RM					145:146	RM 8761	145:151	RM 8761	145:151	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	0	36	theme	Reference	125:133	arg1	Material					135:142	the NISTmAb Reference Material	113:142	the NISTmAb Reference Material	113:142	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	5	37	theme	domain	1275:1280	arg1	interface					1282:1290	the CH2/CH3 domain interface	1263:1290	the CH2/CH3 domain interface	1263:1290	Measurements revealed decreases in structural stability correlating with the degree of Fc-glycan structure loss, especially at the CH2/CH3 domain interface.					
32008322	2	38	theme	analytical	415:424	arg1	purpose					426:432	a key analytical purpose	409:432	a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators	409:573	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	3	39	from	RM	664:665	arg1	variants					638:645	Fc-glycan variants	628:645	Fc-glycan variants from the NISTmAb RM	628:665	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	1	40	theme	analytical	268:277	arg1	technologies					279:290	suitable analytical technologies	259:290	suitable analytical technologies	259:290	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	2	41	theme	emerging	505:512	arg1	procedures					525:534	both established and emerging analytical procedures	484:534	both established and emerging analytical procedures	484:534	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	3	42	theme	Fc-glycan	628:636	arg1	variants					638:645	Fc-glycan variants	628:645	Fc-glycan variants from the NISTmAb RM	628:665	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	2	43	theme	Reference	378:386	arg1	RM					399:400	RM	399:400	RM	399:400	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	2	43	theme	Reference	378:386	arg1	materials					388:396	Reference materials	378:396	Reference materials (RM)	378:401	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	0	44	theme	Higher	38:43	arg1	Changes					62:68	Higher Order Structural Changes	38:68	Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761	38:151	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	4	45	theme	measurement	1025:1035	arg1	variability					1037:1047	measurement variability	1025:1047	measurement variability	1025:1047	An alternative statistical approach is described where measurement thresholds that differentiate between measurement variability and significant structural changes were established on the basis of experimental data.					
32008322	6	46	dep	RM	1328:1329	arg1	this					1323:1326	this	1323:1326	this	1323:1326	These data promote the use of this RM and these Fc-glycan variants for establishing the sensitivity of and validating analytical methods for the detection of HOS measurements of mAbs.					
32008322	0	47	theme	Changes	62:68	arg1	Characterization					18:33	Mass Spectrometry Characterization	0:33	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.	0:152	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	1	48	theme	biomolecules	364:375	arg1	structure					339:347	protein higher order structure	318:347	protein higher order structure (HOS) of these biomolecules	318:375	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	1	48	theme	biomolecules	364:375	arg1	HOS					350:352	HOS	350:352	HOS	350:352	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	0	49	theme	Structural	51:60	arg1	Changes					62:68	Higher Order Structural Changes	38:68	Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761	38:151	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	6	50	theme	measurements	1455:1466	arg1	detection					1438:1446	the detection	1434:1446	the detection of HOS measurements of mAbs	1434:1474	These data promote the use of this RM and these Fc-glycan variants for establishing the sensitivity of and validating analytical methods for the detection of HOS measurements of mAbs.					
32008322	2	51	theme	drug	545:548	arg1	producers					550:558	drug producers	545:558	drug producers	545:558	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	0	52	dep	Higher	38:43	arg1	Order					45:49	Order	45:49	Order	45:49	Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761.					
32008322	6	53	theme	Fc-glycan	1341:1349	arg1	variants					1351:1358	these Fc-glycan variants	1335:1358	these Fc-glycan variants	1335:1358	These data promote the use of this RM and these Fc-glycan variants for establishing the sensitivity of and validating analytical methods for the detection of HOS measurements of mAbs.					
32008322	3	54	theme	NISTmAb	656:662	arg1	RM					664:665	the NISTmAb RM	652:665	the NISTmAb RM	652:665	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	3	55	theme	simple	586:591	arg1	protocols					603:611	two simple enzymatic protocols	582:611	two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM	582:665	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	1	56	theme	dominant	206:213	arg1	class					215:219	a dominant class	204:219	a dominant class of therapeutics	204:235	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	2	57	dep	suitability	454:464	arg1	the					450:452	the	450:452	the	450:452	Reference materials (RM) serve a key analytical purpose of benchmarking the suitability and robustness of both established and emerging analytical procedures for both drug producers and regulators.					
32008322	3	58	theme	enzymatic	593:601	arg1	protocols					603:611	two simple enzymatic protocols	582:611	two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM	582:665	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	4	59	theme	data	1130:1133	arg1	basis					1108:1112	the basis	1104:1112	the basis of experimental data	1104:1133	An alternative statistical approach is described where measurement thresholds that differentiate between measurement variability and significant structural changes were established on the basis of experimental data.					
32008322	4	60	theme	measurement	975:985	arg1	thresholds					987:996	measurement thresholds	975:996	measurement thresholds that differentiate between measurement variability and significant structural changes	975:1082	An alternative statistical approach is described where measurement thresholds that differentiate between measurement variability and significant structural changes were established on the basis of experimental data.					
32008322	6	61	theme	HOS	1451:1453	arg1	measurements					1455:1466	HOS measurements	1451:1466	HOS measurements of mAbs	1451:1474	These data promote the use of this RM and these Fc-glycan variants for establishing the sensitivity of and validating analytical methods for the detection of HOS measurements of mAbs.					
32008322	4	62	theme	experimental	1117:1128	arg1	data					1130:1133	experimental data	1117:1133	experimental data	1117:1133	An alternative statistical approach is described where measurement thresholds that differentiate between measurement variability and significant structural changes were established on the basis of experimental data.					
32008322	3	63	theme	Fc-glycan	751:759	arg1	variants					761:768	these Fc-glycan variants	745:768	these Fc-glycan variants	745:768	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	1	64	from	changes	307:313	arg1	structure					339:347	protein higher order structure	318:347	protein higher order structure (HOS) of these biomolecules	318:375	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	1	64	from	changes	307:313	arg1	HOS					350:352	HOS	350:352	HOS	350:352	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
32008322	4	65	theme	significant	1053:1063	arg1	changes					1076:1082	significant structural changes	1053:1082	significant structural changes	1053:1082	An alternative statistical approach is described where measurement thresholds that differentiate between measurement variability and significant structural changes were established on the basis of experimental data.					
32008322	3	66	theme	mobility	857:864	arg1	IMS-MS					898:903	IMS-MS	898:903	IMS-MS	898:903	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	3	66	theme	mobility	857:864	arg1	spectrometry					884:895	ion mobility spectrometry-mass spectrometry	853:895	ion mobility spectrometry-mass spectrometry (IMS-MS)	853:904	Here, two simple enzymatic protocols for generating Fc-glycan variants from the NISTmAb RM are described and both global and localized changes in HOS between the RM and these Fc-glycan variants are characterized using hydrogen deuterium exchange-mass spectrometry (HDX-MS) and ion mobility spectrometry-mass spectrometry (IMS-MS) measurements.					
32008322	1	67	theme	therapeutics	224:235	arg1	class					215:219	a dominant class	204:219	a dominant class of therapeutics	204:235	As monoclonal antibodies (mAbs) rapidly emerge as a dominant class of therapeutics, so does the need for suitable analytical technologies to monitor for changes in protein higher order structure (HOS) of these biomolecules.					
34288820	0	0	theme	adjuvant	105:112	arg1	activity					114:121	adjuvant activity	105:121	adjuvant activity to promote IgA antibody responses	105:155	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses.					
34288820	5	1	theme	higher	1191:1196	arg1	ability					1198:1204	1.8-fold higher ability	1182:1204	1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS	1182:1287	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
34288820	6	2	theme	Gtf2-S-glucan	1564:1576	arg1	role					1556:1559	a pivotal role	1546:1559	a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS	1546:1611	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	6	3	theme	antigen-specific	1474:1489	arg1	antibodies					1491:1500	higher antigen-specific antibodies	1467:1500	higher antigen-specific antibodies	1467:1500	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	6	4	theme	mice	1350:1353	arg1	administration					1332:1345	intranasal administration	1321:1345	intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS	1321:1413	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	4	5	theme	α-1,6-	897:902	arg1	residues					922:929	α-1,6- and α-1,3-glucose residues	897:929	α-1,6- and α-1,3-glucose residues	897:929	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	6	6	theme	higher	1467:1472	arg1	antibodies					1491:1500	higher antigen-specific antibodies	1467:1500	higher antigen-specific antibodies	1467:1500	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	4	7	theme	residues	922:929	arg1	percentages					882:892	approximately equal percentages	862:892	approximately equal percentages of α-1,6- and α-1,3-glucose residues	862:929	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	5	8	from	cells	1260:1264	arg1	production					1223:1232	IgA production	1219:1232	IgA production from murine Peyer's patch cells than native NTM048 EPS	1219:1287	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
34288820	5	9	theme	native	1271:1276	arg1	EPS					1285:1287	native NTM048 EPS	1271:1287	native NTM048 EPS	1271:1287	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
34288820	5	10	theme	IgA	1219:1221	arg1	production					1223:1232	IgA production	1219:1232	IgA production from murine Peyer's patch cells than native NTM048 EPS	1219:1287	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
34288820	4	11	theme	α-1,3-glucose	908:920	arg1	residues					922:929	α-1,6- and α-1,3-glucose residues	897:929	α-1,6- and α-1,3-glucose residues	897:929	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	4	12	theme	enzyme	751:756	arg1	Gtf1					758:761	the recombinant enzyme Gtf1	735:761	the recombinant enzyme Gtf1	735:761	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	4	13	theme	recombinant	739:749	arg1	Gtf1					758:761	the recombinant enzyme Gtf1	735:761	the recombinant enzyme Gtf1	735:761	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	0	14	theme	IgA	134:136	arg1	responses					147:155	IgA antibody responses	134:155	IgA antibody responses	134:155	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses.					
34288820	4	15	theme	equal	876:880	arg1	percentages					882:892	approximately equal percentages	862:892	approximately equal percentages of α-1,6- and α-1,3-glucose residues	862:929	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	3	16	theme	key	497:499	arg1	structure					509:517	the key polymer structure	493:517	the key polymer structure for adjuvanticity	493:535	To elucidate the key polymer structure for adjuvanticity, two genes, gtf1 and gtf2, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in Escherichia coli.					
34288820	4	17	theme	Glycosyl-linkage	668:683	arg1	analysis					697:704	Glycosyl-linkage composition analysis	668:704	Glycosyl-linkage composition analysis	668:704	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	6	18	theme	NTM048	1404:1409	arg1	EPS					1411:1413	NTM048 EPS	1404:1413	NTM048 EPS	1404:1413	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	6	19	with	EPS	1411:1413	arg1	ovalbumin					1372:1380	ovalbumin	1372:1380	ovalbumin	1372:1380	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	6	19	with	EPS	1411:1413	arg1	Gtf2-S-glucan					1387:1399	Gtf2-S-glucan	1387:1399	Gtf2-S-glucan	1387:1399	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	6	19	with	EPS	1411:1413	arg1	antigen					1363:1369	an antigen	1360:1369	an antigen (ovalbumin)	1360:1381	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	4	20	theme	α-1,6-glucan	790:801	arg1	form					782:785	a soluble form	772:785	a soluble form of α-1,6-glucan	772:801	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	6	21	theme	pivotal	1548:1554	arg1	role					1556:1559	a pivotal role	1546:1559	a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS	1546:1611	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	2	22	theme	glucose	418:424	arg1	polymers					426:433	glucose polymers	418:433	glucose polymers	418:433	Strain NTM048 includes three putative EPS-synthesizing genes, gtf1 and gtf2 for synthesizing glucose polymers, and lvnS for synthesizing fructose polymer.					
34288820	0	23	theme	synthesized	14:24	arg1	exopolysaccharide					26:42	Enzymatically synthesized exopolysaccharide	0:42	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048	0:97	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses.					
34288820	6	24	with	mice	1350:1353	arg1	ovalbumin					1372:1380	ovalbumin	1372:1380	ovalbumin	1372:1380	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	6	24	with	mice	1350:1353	arg1	Gtf2-S-glucan					1387:1399	Gtf2-S-glucan	1387:1399	Gtf2-S-glucan	1387:1399	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	6	24	with	mice	1350:1353	arg1	antigen					1363:1369	an antigen	1360:1369	an antigen (ovalbumin)	1360:1381	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	6	25	theme	EPS	1411:1413	arg1	administration					1332:1345	intranasal administration	1321:1345	intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS	1321:1413	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	4	26	theme	composition	685:695	arg1	analysis					697:704	Glycosyl-linkage composition analysis	668:704	Glycosyl-linkage composition analysis	668:704	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	2	27	theme	fructose	462:469	arg1	polymer					471:477	fructose polymer	462:477	fructose polymer	462:477	Strain NTM048 includes three putative EPS-synthesizing genes, gtf1 and gtf2 for synthesizing glucose polymers, and lvnS for synthesizing fructose polymer.					
34288820	4	28	theme	enzyme	828:833	arg1	Gtf2					835:838	the recombinant enzyme Gtf2	812:838	the recombinant enzyme Gtf2	812:838	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	3	29	theme	hydrolase	598:606	arg1	genes					625:629	glycoside hydrolase family 70 enzyme genes	588:629	glycoside hydrolase family 70 enzyme genes	588:629	To elucidate the key polymer structure for adjuvanticity, two genes, gtf1 and gtf2, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in Escherichia coli.					
34288820	3	29	theme	hydrolase	598:606	arg1	genes					542:546	two genes	538:546	two genes	538:546	To elucidate the key polymer structure for adjuvanticity, two genes, gtf1 and gtf2, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in Escherichia coli.					
34288820	2	30	theme	Strain	325:330	arg1	NTM048					332:337	Strain NTM048	325:337	Strain NTM048	325:337	Strain NTM048 includes three putative EPS-synthesizing genes, gtf1 and gtf2 for synthesizing glucose polymers, and lvnS for synthesizing fructose polymer.					
34288820	1	31	theme	mucosal	304:310	arg1	vaccination					312:322	mucosal vaccination	304:322	mucosal vaccination	304:322	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	0	32	theme	antibody	138:145	arg1	responses					147:155	IgA antibody responses	134:155	IgA antibody responses	134:155	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses.					
34288820	1	33	theme	glucose	234:240	arg1	polymers					242:249	glucose polymers 94% and fructose polymers 6%	234:278	polymers	242:249	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	0	34	theme	probiotic	49:57	arg1	strain					59:64	a probiotic strain	47:64	a probiotic strain Leuconostoc mesenteroides NTM048	47:97	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses.					
34288820	3	35	theme	family	608:613	arg1	genes					625:629	glycoside hydrolase family 70 enzyme genes	588:629	glycoside hydrolase family 70 enzyme genes	588:629	To elucidate the key polymer structure for adjuvanticity, two genes, gtf1 and gtf2, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in Escherichia coli.					
34288820	3	35	theme	family	608:613	arg1	genes					542:546	two genes	538:546	two genes	538:546	To elucidate the key polymer structure for adjuvanticity, two genes, gtf1 and gtf2, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in Escherichia coli.					
34288820	4	36	theme	NMR	710:712	arg1	analysis					714:721	NMR analysis	710:721	NMR analysis	710:721	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	5	37	contain	possessed	1172:1180	arg2	ability					1198:1204	1.8-fold higher ability	1182:1204	1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS	1182:1287	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
34288820	5	37	contain	possessed	1172:1180	arg1	Gtf2-S-glucan					1079:1091	Gtf2-S-glucan	1079:1091	Gtf2-S-glucan	1079:1091	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
34288820	1	38	dep	EPS	229:231	arg1	polymers					268:275	fructose polymers	259:275	fructose polymers	259:275	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	1	38	dep	EPS	229:231	arg1	polymers					242:249	glucose polymers 94% and fructose polymers 6%	234:278	polymers	242:249	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	0	39	theme	Leuconostoc	66:76	arg1	mesenteroides					78:90	Leuconostoc mesenteroides	66:90	a probiotic strain Leuconostoc mesenteroides NTM048	47:97	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses.					
34288820	6	40	theme	intranasal	1321:1330	arg1	administration					1332:1345	intranasal administration	1321:1345	intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS	1321:1413	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	3	41	theme	glycoside	588:596	arg1	hydrolase					598:606	glycoside hydrolase	588:606	glycoside hydrolase family 70 enzyme genes	588:629	To elucidate the key polymer structure for adjuvanticity, two genes, gtf1 and gtf2, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in Escherichia coli.					
34288820	6	42	theme	EPS	1609:1611	arg1	adjuvanticity					1585:1597	the adjuvanticity	1581:1597	the adjuvanticity of NTM048 EPS	1581:1611	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	0	43	theme	strain	59:64	arg1	NTM048					92:97	a probiotic strain Leuconostoc mesenteroides NTM048	47:97	a probiotic strain Leuconostoc mesenteroides NTM048	47:97	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses.					
34288820	5	44	theme	7.8 µm	1164:1169	arg1	particles					1151:1159	particles	1151:1159	particles of 7.8 µm	1151:1169	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
34288820	1	45	dep	exopolysaccharide	210:226	arg1	EPS					229:231	EPS	229:231	EPS; glucose polymers 94% and fructose polymers 6%	229:278	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	6	46	theme	NTM048	1602:1607	arg1	EPS					1609:1611	NTM048 EPS	1602:1611	NTM048 EPS	1602:1611	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	2	47	theme	EPS-synthesizing	363:378	arg1	genes					380:384	three putative EPS-synthesizing genes	348:384	three putative EPS-synthesizing genes	348:384	Strain NTM048 includes three putative EPS-synthesizing genes, gtf1 and gtf2 for synthesizing glucose polymers, and lvnS for synthesizing fructose polymer.					
34288820	5	48	theme	NTM048	1278:1283	arg1	EPS					1285:1287	native NTM048 EPS	1271:1287	native NTM048 EPS	1271:1287	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
34288820	5	49	theme	murine	1239:1244	arg1	cells					1260:1264	murine Peyer's patch cells	1239:1264	murine Peyer's patch cells than native NTM048 EPS	1239:1287	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
34288820	6	50	theme	adjuvanticity	1304:1316	arg1	Evaluation					1290:1299	Evaluation	1290:1299	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS	1290:1413	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	1	51	dep	polymers	242:249	arg1	%					278:278	6%	277:278	6%	277:278	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	1	51	dep	polymers	242:249	arg1	%					253:253	94%	251:253	94%	251:253	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	2	52	theme	putative	354:361	arg1	genes					380:384	three putative EPS-synthesizing genes	348:384	three putative EPS-synthesizing genes	348:384	Strain NTM048 includes three putative EPS-synthesizing genes, gtf1 and gtf2 for synthesizing glucose polymers, and lvnS for synthesizing fructose polymer.					
34288820	4	53	theme	soluble	774:780	arg1	form					782:785	a soluble form	772:785	a soluble form of α-1,6-glucan	772:801	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	1	54	with	exopolysaccharide	210:226	arg1	adjuvanticity					286:298	adjuvanticity	286:298	adjuvanticity	286:298	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	5	55	theme	polysaccharides	1013:1027	arg1	Comparison					999:1008	Comparison	999:1008	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS	999:1063	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
34288820	6	56	from	role	1556:1559	arg1	adjuvanticity					1585:1597	the adjuvanticity	1581:1597	the adjuvanticity of NTM048 EPS	1581:1611	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	1	57	theme	Leuconostoc	158:168	arg1	mesenteroides					170:182	Leuconostoc mesenteroides	158:182	Leuconostoc mesenteroides strain NTM048	158:196	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	3	58	theme	polymer	501:507	arg1	structure					509:517	the key polymer structure	493:517	the key polymer structure for adjuvanticity	493:535	To elucidate the key polymer structure for adjuvanticity, two genes, gtf1 and gtf2, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in Escherichia coli.					
34288820	0	59	theme	NTM048	92:97	arg1	exopolysaccharide					26:42	Enzymatically synthesized exopolysaccharide	0:42	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048	0:97	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses.					
34288820	3	60	theme	enzyme	618:623	arg1	genes					625:629	glycoside hydrolase family 70 enzyme genes	588:629	glycoside hydrolase family 70 enzyme genes	588:629	To elucidate the key polymer structure for adjuvanticity, two genes, gtf1 and gtf2, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in Escherichia coli.					
34288820	3	60	theme	enzyme	618:623	arg1	genes					542:546	two genes	538:546	two genes	538:546	To elucidate the key polymer structure for adjuvanticity, two genes, gtf1 and gtf2, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in Escherichia coli.					
34288820	6	61	theme	antibodies	1491:1500	arg1	production					1453:1462	the production	1449:1462	the production of higher antigen-specific antibodies	1449:1500	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	1	62	theme	mesenteroides	170:182	arg1	NTM048					191:196	Leuconostoc mesenteroides strain NTM048	158:196	Leuconostoc mesenteroides strain NTM048	158:196	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	6	63	theme	airway	1509:1514	arg1	mucosa					1516:1521	the airway mucosa	1505:1521	the airway mucosa	1505:1521	Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.					
34288820	1	64	theme	fructose	259:266	arg1	polymers					268:275	fructose polymers	259:275	fructose polymers	259:275	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	0	65	theme	mesenteroides	78:90	arg1	NTM048					92:97	a probiotic strain Leuconostoc mesenteroides NTM048	47:97	a probiotic strain Leuconostoc mesenteroides NTM048	47:97	Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses.					
34288820	4	66	theme	recombinant	816:826	arg1	Gtf2					835:838	the recombinant enzyme Gtf2	812:838	the recombinant enzyme Gtf2	812:838	Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan).					
34288820	1	67	theme	strain	184:189	arg1	NTM048					191:196	Leuconostoc mesenteroides strain NTM048	158:196	Leuconostoc mesenteroides strain NTM048	158:196	Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination.					
34288820	5	68	theme	patch	1254:1258	arg1	cells					1260:1264	murine Peyer's patch cells	1239:1264	murine Peyer's patch cells than native NTM048 EPS	1239:1287	Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS.					
33231436	0	0	theme	Weight	173:178	arg1	Trisaccharide					180:192	a Small Molecular Weight Trisaccharide	155:192	a Small Molecular Weight Trisaccharide	155:192	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	4	1	from	level	697:701	arg1	functions					627:635	the functions	623:635	the functions of the individual antennary form of N-glycan at a molecular level	623:701	Thus, the functions of the individual antennary form of N-glycan at a molecular level remain unclear.					
33231436	1	2	theme	structure	262:270	arg1	modulation					244:253	the modulation	240:253	the modulation of the structure and biological activity of glycoproteins	240:311	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	1	3	theme	biological	276:285	arg1	activity					287:294	biological activity	276:294	biological activity	276:294	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	4	4	theme	N-glycan	673:680	arg1	form					665:668	the individual antennary form	640:668	the individual antennary form of N-glycan	640:680	Thus, the functions of the individual antennary form of N-glycan at a molecular level remain unclear.					
33231436	1	5	gly	glycosylation	199:211	arg1	structure					262:270	structure	262:270	structure	262:270	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	1	5	gly	glycosylation	199:211	arg1	activity					287:294	biological activity	276:294	biological activity	276:294	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	1	5	gly	glycosylation	199:211	arg1	proteins					216:223	proteins	216:223	proteins	216:223	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	0	6	theme	Chemical	0:7	arg1	Synthesis					9:17	Chemical Synthesis	0:17	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.	0:193	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	3	7	theme	form	598:601	arg1	alteration					570:579	the intentional alteration	554:579	the intentional alteration of the antennary form of N-glycans	554:614	However, there are no chemical or biological methods to obtain homogeneous glycoproteins via the intentional alteration of the antennary form of N-glycans.					
33231436	5	8	theme	biantennary	863:873	arg1	sialylglycans					875:887	two biantennary sialylglycans	859:887	two biantennary sialylglycans at both positions 24 and 38	859:915	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	4	9	theme	individual	644:653	arg1	form					665:668	the individual antennary form	640:668	the individual antennary form of N-glycan	640:680	Thus, the functions of the individual antennary form of N-glycan at a molecular level remain unclear.					
33231436	7	10	theme	introduced	1265:1274	arg1	position					1276:1283	the introduced position	1261:1283	the introduced position	1261:1283	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	7	10	theme	introduced	1265:1274	arg1	distant					1298:1304	distant	1298:1304	distant	1298:1304	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	6	11	theme	condensation	957:968	arg1	reactions					970:978	efficient liquid-phase condensation reactions	934:978	efficient liquid-phase condensation reactions	934:978	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	4	12	theme	form	665:668	arg1	functions					627:635	the functions	623:635	the functions of the individual antennary form of N-glycan at a molecular level	623:701	Thus, the functions of the individual antennary form of N-glycan at a molecular level remain unclear.					
33231436	7	13	theme	molecular	1173:1181	arg1	glycoform					1246:1254	the EPO glycoform	1238:1254	the EPO glycoform	1238:1254	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	7	13	theme	molecular	1173:1181	arg1	weight					1183:1188	The molecular weight	1169:1188	The molecular weight of the newly added antennary element	1169:1225	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	7	13	theme	molecular	1173:1181	arg1	%					1233:1233	∼3%	1231:1233	∼3% of the EPO glycoform	1231:1254	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	6	14	theme	efficient	934:942	arg1	reactions					970:978	efficient liquid-phase condensation reactions	934:978	efficient liquid-phase condensation reactions	934:978	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	3	15	theme	N-glycans	606:614	arg1	form					598:601	the antennary form	584:601	the antennary form of N-glycans	584:614	However, there are no chemical or biological methods to obtain homogeneous glycoproteins via the intentional alteration of the antennary form of N-glycans.					
33231436	5	16	from	position	835:842	arg1	sialylglycans					875:887	two biantennary sialylglycans	859:887	two biantennary sialylglycans at both positions 24 and 38	859:915	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	5	16	from	position	835:842	arg1	sialylglycan					819:830	a triantennary sialylglycan	804:830	a triantennary sialylglycan at position 83	804:845	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	7	17	gly	glycoform	1246:1254	arg1	EPO					1242:1244	the EPO glycoform	1238:1254	the EPO glycoform	1238:1254	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	0	18	theme	Small	157:161	arg1	Weight					173:178	a Small Molecular Weight	155:178	a Small Molecular Weight Trisaccharide	155:192	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	8	19	theme	native	1518:1523	arg1	EPO					1525:1527	a commercially available native EPO	1493:1527	a commercially available native EPO	1493:1527	However, in vivo assays using mice revealed that the additional antennary element at position 83 dramatically increased the hematopoietic activity compared to a commercially available native EPO.					
33231436	0	20	theme	Triantennary	59:70	arg1	N-Glycan					72:79	a Triantennary N-Glycan	57:79	a Triantennary N-Glycan	57:79	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	6	21	theme	triantennary	1021:1032	arg1	N-glycan					1034:1041	a triantennary N-glycan	1019:1041	a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions	1019:1166	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	7	22	theme	glycoform	1246:1254	arg1	glycoform					1246:1254	the EPO glycoform	1238:1254	the EPO glycoform	1238:1254	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	7	22	theme	glycoform	1246:1254	arg1	weight					1183:1188	The molecular weight	1169:1188	The molecular weight of the newly added antennary element	1169:1225	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	7	22	theme	glycoform	1246:1254	arg1	%					1233:1233	∼3%	1231:1233	∼3% of the EPO glycoform	1231:1254	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	7	23	theme	EPO	1242:1244	arg1	glycoform					1246:1254	the EPO glycoform	1238:1254	the EPO glycoform	1238:1254	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	0	24	theme	Biological	104:113	arg1	Activity					115:122	Biological Activity	104:122	Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide	104:192	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	9	25	theme	N-glycans	1602:1610	arg1	pattern					1591:1597	the antennary pattern	1577:1597	the antennary pattern of N-glycans	1577:1610	These unprecedented data clearly indicate that the antennary pattern of N-glycans inherently plays a critical role in the modulation of protein functions.					
33231436	5	26	theme	triantennary	806:817	arg1	sialylglycan					819:830	a triantennary sialylglycan	804:830	a triantennary sialylglycan at position 83	804:845	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	1	27	theme	activity	287:294	arg1	modulation					244:253	the modulation	240:253	the modulation of the structure and biological activity of glycoproteins	240:311	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	1	28	dep	structure	262:270	arg1	the					258:260	the	258:260	the	258:260	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	6	29	theme	Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc	1073:1101	arg1	element					1103:1109	a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element	1071:1109	a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element	1071:1109	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	6	30	contain	having	1012:1017	arg2	N-glycan					1034:1041	a triantennary N-glycan	1019:1041	a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions	1019:1166	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	6	30	contain	having	1012:1017	arg1	sialylglycopeptide					993:1010	a sialylglycopeptide	991:1010	a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions	991:1166	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	7	31	theme	antennary	1209:1217	arg1	element					1219:1225	the newly added antennary element	1193:1225	the newly added antennary element	1193:1225	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	1	32	theme	glycoproteins	299:311	arg1	structure					262:270	structure	262:270	structure	262:270	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	1	32	theme	glycoproteins	299:311	arg1	activity					287:294	biological activity	276:294	biological activity	276:294	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	9	33	theme	critical	1631:1638	arg1	role					1640:1643	a critical role	1629:1643	a critical role	1629:1643	These unprecedented data clearly indicate that the antennary pattern of N-glycans inherently plays a critical role in the modulation of protein functions.					
33231436	5	34	contain	having	797:802	arg1	glycoform					787:795	an erythropoietin (EPO) glycoform	763:795	an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38	763:915	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	5	34	contain	having	797:802	arg2	sialylglycans					875:887	two biantennary sialylglycans	859:887	two biantennary sialylglycans at both positions 24 and 38	859:915	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	5	34	contain	having	797:802	arg2	sialylglycan					819:830	a triantennary sialylglycan	804:830	a triantennary sialylglycan at position 83	804:845	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	0	35	theme	Erythropoietin	25:38	arg1	Glycoform					40:48	an Erythropoietin Glycoform	22:48	an Erythropoietin Glycoform Having a Triantennary N-Glycan	22:79	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	8	36	dep	in	1343:1344	arg1	vivo					1346:1349	vivo	1346:1349	vivo	1346:1349	However, in vivo assays using mice revealed that the additional antennary element at position 83 dramatically increased the hematopoietic activity compared to a commercially available native EPO.					
33231436	7	37	theme	bioactive	1315:1323	arg1	protein					1325:1331	the bioactive protein	1311:1331	the bioactive protein	1311:1331	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	2	38	gly	glycoproteins	355:367	arg1	glycoproteins					355:367	glycoproteins	355:367	glycoproteins	355:367	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	3	39	theme	biological	495:504	arg1	methods					506:512	no chemical or biological methods	480:512	no chemical or biological methods	480:512	However, there are no chemical or biological methods to obtain homogeneous glycoproteins via the intentional alteration of the antennary form of N-glycans.					
33231436	7	40	from	protein	1325:1331	arg1	position					1276:1283	the introduced position	1261:1283	the introduced position	1261:1283	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	7	40	from	protein	1325:1331	arg1	distant					1298:1304	distant	1298:1304	distant	1298:1304	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	6	41	gly	sialylglycopeptide	993:1010	arg2	sialylglycopeptide					993:1010	a sialylglycopeptide	991:1010	a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions	991:1166	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	3	42	theme	chemical	483:490	arg1	methods					506:512	no chemical or biological methods	480:512	no chemical or biological methods	480:512	However, there are no chemical or biological methods to obtain homogeneous glycoproteins via the intentional alteration of the antennary form of N-glycans.					
33231436	9	43	theme	protein	1666:1672	arg1	functions					1674:1682	protein functions	1666:1682	protein functions	1666:1682	These unprecedented data clearly indicate that the antennary pattern of N-glycans inherently plays a critical role in the modulation of protein functions.					
33231436	8	44	theme	antennary	1398:1406	arg1	element					1408:1414	the additional antennary element	1383:1414	the additional antennary element at position 83	1383:1429	However, in vivo assays using mice revealed that the additional antennary element at position 83 dramatically increased the hematopoietic activity compared to a commercially available native EPO.					
33231436	8	45	theme	hematopoietic	1458:1470	arg1	activity					1472:1479	the hematopoietic activity	1454:1479	the hematopoietic activity	1454:1479	However, in vivo assays using mice revealed that the additional antennary element at position 83 dramatically increased the hematopoietic activity compared to a commercially available native EPO.					
33231436	3	46	theme	homogeneous	524:534	arg1	glycoproteins					536:548	homogeneous glycoproteins	524:548	homogeneous glycoproteins	524:548	However, there are no chemical or biological methods to obtain homogeneous glycoproteins via the intentional alteration of the antennary form of N-glycans.					
33231436	5	47	theme	erythropoietin	766:779	arg1	glycoform					787:795	an erythropoietin (EPO) glycoform	763:795	an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38	763:915	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	0	48	theme	Trisaccharide	180:192	arg1	Addition					143:150	Addition	143:150	Addition of a Small Molecular Weight Trisaccharide	143:192	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	2	49	theme	diverse	387:393	arg1	tri-					428:431	tri-	428:431	tri-	428:431	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	2	49	theme	diverse	387:393	arg1	bi-					423:425	bi-	423:425	bi-	423:425	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	2	49	theme	diverse	387:393	arg1	forms					454:458	tetra-antennary forms	438:458	tetra-antennary forms	438:458	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	2	49	theme	diverse	387:393	arg1	patterns					405:412	diverse antennary patterns	387:412	diverse antennary patterns	387:412	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	6	50	theme	biantennary	1118:1128	arg1	glycan					1130:1135	the biantennary glycan	1114:1135	the biantennary glycan	1114:1135	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	3	51	theme	intentional	558:568	arg1	alteration					570:579	the intentional alteration	554:579	the intentional alteration of the antennary form of N-glycans	554:614	However, there are no chemical or biological methods to obtain homogeneous glycoproteins via the intentional alteration of the antennary form of N-glycans.					
33231436	8	52	from	position	1419:1426	arg1	element					1408:1414	the additional antennary element	1383:1414	the additional antennary element at position 83	1383:1429	However, in vivo assays using mice revealed that the additional antennary element at position 83 dramatically increased the hematopoietic activity compared to a commercially available native EPO.					
33231436	3	53	theme	antennary	588:596	arg1	form					598:601	the antennary form	584:601	the antennary form of N-glycans	584:614	However, there are no chemical or biological methods to obtain homogeneous glycoproteins via the intentional alteration of the antennary form of N-glycans.					
33231436	0	54	theme	Glycoprotein	127:138	arg1	Activity					115:122	Biological Activity	104:122	Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide	104:192	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	1	55	theme	proteins	216:223	arg1	glycosylation					199:211	The glycosylation	195:211	The glycosylation of proteins	195:223	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	6	56	theme	liquid-phase	944:955	arg1	reactions					970:978	efficient liquid-phase condensation reactions	934:978	efficient liquid-phase condensation reactions	934:978	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	4	57	theme	antennary	655:663	arg1	form					665:668	the individual antennary form	640:668	the individual antennary form of N-glycan	640:680	Thus, the functions of the individual antennary form of N-glycan at a molecular level remain unclear.					
33231436	2	58	link	Asparagine-linked	314:330	arg1	glycans					332:338	Asparagine-linked glycans	314:338	Asparagine-linked glycans (N-glycans) of glycoproteins	314:367	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	2	58	link	Asparagine-linked	314:330	arg1	N-glycans					341:349	N-glycans	341:349	N-glycans	341:349	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	8	59	theme	available	1508:1516	arg1	EPO					1525:1527	a commercially available native EPO	1493:1527	a commercially available native EPO	1493:1527	However, in vivo assays using mice revealed that the additional antennary element at position 83 dramatically increased the hematopoietic activity compared to a commercially available native EPO.					
33231436	0	60	theme	Molecular	163:171	arg1	Weight					173:178	a Small Molecular Weight	155:178	a Small Molecular Weight Trisaccharide	155:192	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	1	61	gly	glycoproteins	299:311	arg1	glycoproteins					299:311	glycoproteins	299:311	glycoproteins	299:311	The glycosylation of proteins contributes to the modulation of the structure and biological activity of glycoproteins.					
33231436	9	62	theme	unprecedented	1536:1548	arg1	data					1550:1553	These unprecedented data	1530:1553	These unprecedented data	1530:1553	These unprecedented data clearly indicate that the antennary pattern of N-glycans inherently plays a critical role in the modulation of protein functions.					
33231436	3	63	gly	glycoproteins	536:548	arg1	glycoproteins					536:548	homogeneous glycoproteins	524:548	homogeneous glycoproteins	524:548	However, there are no chemical or biological methods to obtain homogeneous glycoproteins via the intentional alteration of the antennary form of N-glycans.					
33231436	4	64	theme	molecular	687:695	arg1	level					697:701	a molecular level	685:701	a molecular level	685:701	Thus, the functions of the individual antennary form of N-glycan at a molecular level remain unclear.					
33231436	0	65	theme	Significant	82:92	arg1	Change					94:99	Significant Change	82:99	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.	0:193	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	8	66	theme	in	1343:1344	arg1	assays					1351:1356	in vivo assays	1343:1356	in vivo assays using mice	1343:1367	However, in vivo assays using mice revealed that the additional antennary element at position 83 dramatically increased the hematopoietic activity compared to a commercially available native EPO.					
33231436	5	67	from	positions	897:905	arg1	sialylglycans					875:887	two biantennary sialylglycans	859:887	two biantennary sialylglycans at both positions 24 and 38	859:915	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	5	67	from	positions	897:905	arg1	sialylglycan					819:830	a triantennary sialylglycan	804:830	a triantennary sialylglycan at position 83	804:845	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	9	68	theme	antennary	1581:1589	arg1	pattern					1591:1597	the antennary pattern	1577:1597	the antennary pattern of N-glycans	1577:1610	These unprecedented data clearly indicate that the antennary pattern of N-glycans inherently plays a critical role in the modulation of protein functions.					
33231436	2	69	theme	Asparagine-linked	314:330	arg1	glycans					332:338	Asparagine-linked glycans	314:338	Asparagine-linked glycans (N-glycans) of glycoproteins	314:367	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	2	69	theme	Asparagine-linked	314:330	arg1	N-glycans					341:349	N-glycans	341:349	N-glycans	341:349	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	0	70	theme	Activity	115:122	arg1	Change					94:99	Significant Change	82:99	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.	0:193	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	5	71	theme	chemical	741:748	arg1	synthesis					750:758	the chemical synthesis	737:758	the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38	737:915	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	7	72	theme	element	1219:1225	arg1	glycoform					1246:1254	the EPO glycoform	1238:1254	the EPO glycoform	1238:1254	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	7	72	theme	element	1219:1225	arg1	weight					1183:1188	The molecular weight	1169:1188	The molecular weight of the newly added antennary element	1169:1225	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	7	72	theme	element	1219:1225	arg1	%					1233:1233	∼3%	1231:1233	∼3% of the EPO glycoform	1231:1254	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	6	73	theme	element	1103:1109	arg1	addition					1059:1066	the addition	1055:1066	the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan	1055:1135	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	5	74	dep	positions	897:905	arg1	38					914:915	38	914:915	38	914:915	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	5	74	dep	positions	897:905	arg1	24					907:908	24	907:908	24	907:908	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	7	75	theme	added	1203:1207	arg1	element					1219:1225	the newly added antennary element	1193:1225	the newly added antennary element	1193:1225	The molecular weight of the newly added antennary element was ∼3% of the EPO glycoform, and the introduced position was the most distant from the bioactive protein.					
33231436	0	76	dep	Synthesis	9:17	arg1	Change					94:99	Significant Change	82:99	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.	0:193	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	2	77	theme	tetra-antennary	438:452	arg1	forms					454:458	tetra-antennary forms	438:458	tetra-antennary forms	438:458	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	8	78	theme	additional	1387:1396	arg1	element					1408:1414	the additional antennary element	1383:1414	the additional antennary element at position 83	1383:1429	However, in vivo assays using mice revealed that the additional antennary element at position 83 dramatically increased the hematopoietic activity compared to a commercially available native EPO.					
33231436	0	79	theme	Glycoform	40:48	arg1	Synthesis					9:17	Chemical Synthesis	0:17	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.	0:193	Chemical Synthesis of an Erythropoietin Glycoform Having a Triantennary N-Glycan: Significant Change of Biological Activity of Glycoprotein by Addition of a Small Molecular Weight Trisaccharide.					
33231436	5	80	theme	glycoform	787:795	arg1	synthesis					750:758	the chemical synthesis	737:758	the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38	737:915	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	2	81	theme	glycoproteins	355:367	arg1	glycans					332:338	Asparagine-linked glycans	314:338	Asparagine-linked glycans (N-glycans) of glycoproteins	314:367	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	2	81	theme	glycoproteins	355:367	arg1	N-glycans					341:349	N-glycans	341:349	N-glycans	341:349	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	9	82	theme	functions	1674:1682	arg1	modulation					1652:1661	the modulation	1648:1661	the modulation of protein functions	1648:1682	These unprecedented data clearly indicate that the antennary pattern of N-glycans inherently plays a critical role in the modulation of protein functions.					
33231436	6	83	theme	semisynthetic	1143:1155	arg1	conditions					1157:1166	semisynthetic conditions	1143:1166	semisynthetic conditions	1143:1166	We demonstrated efficient liquid-phase condensation reactions to prepare a sialylglycopeptide having a triantennary N-glycan prepared by the addition of a Neu5Ac-α-2,6-Gal-β-1,4-GlcNAc element to the biantennary glycan under semisynthetic conditions.					
33231436	5	84	gly	glycoform	787:795	arg1	EPO					782:784	EPO	782:784	EPO	782:784	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	5	84	gly	glycoform	787:795	arg1	erythropoietin					766:779	an erythropoietin	763:779	an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38	763:915	Herein, we report the chemical synthesis of an erythropoietin (EPO) glycoform having a triantennary sialylglycan at position 83, as well as two biantennary sialylglycans at both positions 24 and 38.					
33231436	2	85	theme	antennary	395:403	arg1	tri-					428:431	tri-	428:431	tri-	428:431	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	2	85	theme	antennary	395:403	arg1	bi-					423:425	bi-	423:425	bi-	423:425	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	2	85	theme	antennary	395:403	arg1	forms					454:458	tetra-antennary forms	438:458	tetra-antennary forms	438:458	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
33231436	2	85	theme	antennary	395:403	arg1	patterns					405:412	diverse antennary patterns	387:412	diverse antennary patterns	387:412	Asparagine-linked glycans (N-glycans) of glycoproteins naturally exhibit diverse antennary patterns, such as bi-, tri-, and tetra-antennary forms.					
31941772	4	0	theme	autologous	772:781	arg1	nAbs					808:811	nAbs	808:811	nAbs	808:811	We isolated autologous neutralizing antibodies (nAbs) and described their structure in complex with the B41 Env.					
31941772	4	0	theme	autologous	772:781	arg1	antibodies					796:805	autologous neutralizing antibodies	772:805	autologous neutralizing antibodies (nAbs)	772:812	We isolated autologous neutralizing antibodies (nAbs) and described their structure in complex with the B41 Env.					
31941772	4	1	with	complex	847:853	arg1	Env					868:870	the B41 Env	860:870	the B41 Env	860:870	We isolated autologous neutralizing antibodies (nAbs) and described their structure in complex with the B41 Env.					
31941772	11	2	from	complex	2084:2090	arg1	studies					2066:2072	Our high-resolution cryo-electron microscopy (cryoEM) studies	2012:2072	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex	2012:2127	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	1	3	gly	glycoprotein	158:169	arg1	Env					172:174	Env	172:174	Env	172:174	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	3	gly	glycoprotein	158:169	arg1	glycoprotein					158:169	A BG505-derived HIV envelope glycoprotein	129:169	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	9	4	theme	cross-reactivity	1534:1549	arg1	lack					1526:1529	the lack	1522:1529	the lack of cross-reactivity in glycan hole antibodies	1522:1575	Our data demonstrate that the lack of cross-reactivity in glycan hole antibodies is due to amino acid differences within the epitope, and our attempts to rationally design cross-reactive trimers resulted in only limited success.					
31941772	9	4	theme	cross-reactivity	1534:1549	arg1	due					1580:1582	due	1580:1582	due	1580:1582	Our data demonstrate that the lack of cross-reactivity in glycan hole antibodies is due to amino acid differences within the epitope, and our attempts to rationally design cross-reactive trimers resulted in only limited success.					
31941772	11	5	theme	antibody	2112:2119	arg1	complex					2121:2127	a B41-specific antibody complex	2097:2127	a B41-specific antibody complex	2097:2127	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	9	6	theme	glycan	1554:1559	arg1	antibodies					1566:1575	glycan hole antibodies	1554:1575	glycan hole antibodies	1554:1575	Our data demonstrate that the lack of cross-reactivity in glycan hole antibodies is due to amino acid differences within the epitope, and our attempts to rationally design cross-reactive trimers resulted in only limited success.					
31941772	11	7	theme	hole	2190:2193	arg1	response					2195:2202	this strain-specific glycan hole response	2162:2202	this strain-specific glycan hole response	2162:2202	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	11	8	theme	cryo-electron	2032:2044	arg1	microscopy					2046:2055	high-resolution cryo-electron microscopy	2016:2055	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex	2012:2127	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	11	8	theme	cryo-electron	2032:2044	arg1	cryoEM					2058:2063	cryoEM	2058:2063	cryoEM	2058:2063	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	6	9	theme	glycan	1054:1059	arg1	antibodies					1066:1075	previously described BG505 glycan hole antibodies	1027:1075	previously described BG505 glycan hole antibodies	1027:1075	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	11	10	theme	strain-specific	2167:2181	arg1	response					2195:2202	this strain-specific glycan hole response	2162:2202	this strain-specific glycan hole response	2162:2202	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	3	11	theme	isoleucine-to-proline	667:687	arg1	[IP					698:700	an isoleucine-to-proline mutation [IP	664:700	an isoleucine-to-proline mutation [IP	664:700	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	6	12	theme	described	1038:1046	arg1	antibodies					1066:1075	previously described BG505 glycan hole antibodies	1027:1075	previously described BG505 glycan hole antibodies	1027:1075	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	6	13	dep	antibodies	1066:1075	arg1	contrast					1015:1022	contrast	1015:1022	contrast	1015:1022	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	10	14	theme	prime-boost	1827:1837	arg1	immunogens					1839:1848	prime-boost immunogens	1827:1848	prime-boost immunogens	1827:1848	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	9	15	theme	amino	1587:1591	arg1	differences					1598:1608	amino acid differences	1587:1608	amino acid differences within the epitope	1587:1627	Our data demonstrate that the lack of cross-reactivity in glycan hole antibodies is due to amino acid differences within the epitope, and our attempts to rationally design cross-reactive trimers resulted in only limited success.					
31941772	12	16	theme	immunodominant	2235:2248	arg1	hole					2257:2260	the immunodominant glycan hole	2231:2260	the immunodominant glycan hole shared between BG505 and B41	2231:2289	We conclude that even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.					
31941772	6	17	theme	N241	1131:1134	arg1	glycan					1136:1141	the >97% conserved N241 glycan	1112:1141	the >97% conserved N241 glycan	1112:1141	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	6	17	theme	N241	1131:1134	arg1	present					1153:1159	present	1153:1159	present	1153:1159	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	7	18	theme	overlapping	1270:1280	arg1	epitopes					1294:1301	overlapping glycan hole epitopes	1270:1301	overlapping glycan hole epitopes	1270:1301	Single-particle cryo-electron microscopy studies confirmed that B41- and BG505-specific nAbs bind to overlapping glycan hole epitopes.					
31941772	3	19	theme	glycan	566:571	arg1	hole					573:576	the common glycan hole	555:576	the common glycan hole	555:576	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	1	20	theme	subtype	121:127	arg1	immunogens					177:186	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	3	21	theme	BG505	738:742	arg1	coimmunization					744:757	BG505 coimmunization	738:757	BG505 coimmunization	738:757	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	8	22	theme	hole	1381:1384	arg1	epitope					1386:1392	the BG505 glycan hole epitope	1364:1392	the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb	1364:1454	We then used our high-resolution data to guide mutations in the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb, but we recovered only partial binding.					
31941772	7	23	theme	BG505-specific	1242:1255	arg1	nAbs					1257:1260	B41- and BG505-specific nAbs	1233:1260	B41- and BG505-specific nAbs	1233:1260	Single-particle cryo-electron microscopy studies confirmed that B41- and BG505-specific nAbs bind to overlapping glycan hole epitopes.					
31941772	1	24	theme	glycosylated	310:321	arg1	trimer					323:328	the heavily glycosylated trimer	298:328	the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289	298:409	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	25	theme	BG505-derived	131:143	arg1	Env					172:174	Env	172:174	Env	172:174	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	25	theme	BG505-derived	131:143	arg1	glycoprotein					158:169	A BG505-derived HIV envelope glycoprotein	129:169	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	2	26	theme	B	448:448	arg1	virus					450:454	a subtype B virus	438:454	a subtype B virus	438:454	The Env derived from B41, a subtype B virus, shares a glycan hole centered on positions 230 and 289.					
31941772	2	26	theme	B	448:448	arg1	B41					433:435	B41	433:435	B41	433:435	The Env derived from B41, a subtype B virus, shares a glycan hole centered on positions 230 and 289.					
31941772	10	27	theme	neutralizing	1932:1943	arg1	epitopes					1945:1952	the most dominant autologous neutralizing epitopes	1903:1952	the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits	1903:2009	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	1	28	theme	envelope	149:156	arg1	Env					172:174	Env	172:174	Env	172:174	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	28	theme	envelope	149:156	arg1	glycoprotein					158:169	A BG505-derived HIV envelope glycoprotein	129:169	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	10	29	theme	dominant	1912:1919	arg1	epitopes					1945:1952	the most dominant autologous neutralizing epitopes	1903:1952	the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits	1903:2009	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	1	30	theme	potential	341:349	arg1	sites					374:378	potential N-linked glycosylation sites	341:378	potential N-linked glycosylation sites	341:378	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	31	located	located	266:272	arg2	located					266:272	located	266:272	located	266:272	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	31	located	located	266:272	arg2	epitope					244:250	the dominant autologous neutralizing epitope	207:250	the dominant autologous neutralizing epitope in rabbits	207:261	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	31	located	located	266:272	arg1	region					288:293	an exposed region	277:293	an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289	277:409	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	4	32	theme	B41	864:866	arg1	Env					868:870	the B41 Env	860:870	the B41 Env	860:870	We isolated autologous neutralizing antibodies (nAbs) and described their structure in complex with the B41 Env.					
31941772	10	33	dep	designing	1817:1825	arg1	remains					1850:1856	remains	1850:1856	designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole	1817:1891	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	1	34	theme	glycosylation	360:372	arg1	sites					374:378	potential N-linked glycosylation sites	341:378	potential N-linked glycosylation sites	341:378	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	35	link	N-linked	351:358	arg1	sites					374:378	potential N-linked glycosylation sites	341:378	potential N-linked glycosylation sites	341:378	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	0	36	theme	Autologous	0:9	arg1	Responses					20:28	Autologous Antibody Responses	0:28	Autologous Antibody Responses to an HIV Envelope Glycan Hole	0:59	Autologous Antibody Responses to an HIV Envelope Glycan Hole Are Not Easily Broadened in Rabbits.					
31941772	1	37	with	studies	108:114	arg1	immunogens					177:186	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	6	38	located	present	1153:1159	arg2	glycan					1136:1141	the >97% conserved N241 glycan	1112:1141	the >97% conserved N241 glycan	1112:1141	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	6	38	located	present	1153:1159	arg1	B41					1164:1166	B41	1164:1166	B41	1164:1166	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	6	38	located	present	1153:1159	arg2	present					1153:1159	present	1153:1159	present	1153:1159	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	9	39	theme	limited	1708:1714	arg1	success					1716:1722	only limited success	1703:1722	only limited success	1703:1722	Our data demonstrate that the lack of cross-reactivity in glycan hole antibodies is due to amino acid differences within the epitope, and our attempts to rationally design cross-reactive trimers resulted in only limited success.					
31941772	8	40	theme	nAb	1452:1454	arg1	reactivity					1423:1432	the reactivity	1419:1432	the reactivity of a B41-specific nAb	1419:1454	We then used our high-resolution data to guide mutations in the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb, but we recovered only partial binding.					
31941772	7	41	theme	cryo-electron	1185:1197	arg1	microscopy					1199:1208	Single-particle cryo-electron microscopy	1169:1208	Single-particle cryo-electron microscopy studies	1169:1216	Single-particle cryo-electron microscopy studies confirmed that B41- and BG505-specific nAbs bind to overlapping glycan hole epitopes.					
31941772	0	42	theme	Envelope	40:47	arg1	Hole					56:59	an HIV Envelope Glycan Hole	33:59	an HIV Envelope Glycan Hole	33:59	Autologous Antibody Responses to an HIV Envelope Glycan Hole Are Not Easily Broadened in Rabbits.					
31941772	8	43	theme	high-resolution	1321:1335	arg1	data					1337:1340	our high-resolution data	1317:1340	our high-resolution data	1317:1340	We then used our high-resolution data to guide mutations in the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb, but we recovered only partial binding.					
31941772	8	44	theme	partial	1479:1485	arg1	binding					1487:1493	only partial binding	1474:1493	only partial binding	1474:1493	We then used our high-resolution data to guide mutations in the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb, but we recovered only partial binding.					
31941772	10	45	theme	immunodominant	1744:1757	arg1	hole					1766:1769	the immunodominant glycan hole	1740:1769	the immunodominant glycan hole shared between BG505 and B41	1740:1798	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	1	46	theme	autologous	220:229	arg1	located					266:272	located	266:272	located	266:272	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	46	theme	autologous	220:229	arg1	epitope					244:250	the dominant autologous neutralizing epitope	207:250	the dominant autologous neutralizing epitope in rabbits	207:261	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	5	47	theme	autologous	904:913	arg1	lineages					919:926	distinct autologous nAb lineages	895:926	distinct autologous nAb lineages	895:926	Our data suggest that distinct autologous nAb lineages are induced by BG505 and B41 immunogens, even when both were administered together.					
31941772	10	48	theme	trimer-immunized	1986:2001	arg1	rabbits					2003:2009	trimer-immunized rabbits	1986:2009	trimer-immunized rabbits	1986:2009	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	11	49	theme	B41-specific	2099:2110	arg1	complex					2121:2127	a B41-specific antibody complex	2097:2127	a B41-specific antibody complex	2097:2127	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	11	50	from	B41	2077:2079	arg1	complex					2084:2090	complex	2084:2090	complex with a B41-specific antibody complex	2084:2127	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	11	51	theme	high-resolution	2016:2030	arg1	microscopy					2046:2055	high-resolution cryo-electron microscopy	2016:2055	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex	2012:2127	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	11	51	theme	high-resolution	2016:2030	arg1	cryoEM					2058:2063	cryoEM	2058:2063	cryoEM	2058:2063	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	3	52	theme	B41	621:623	arg1	SOSIP					625:629	B41 SOSIP	621:629	B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone	621:716	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	3	52	theme	B41	621:623	arg1	[SOS					653:656	gp120-gp41 disulfide [SOS	632:656	gp120-gp41 disulfide [SOS	632:656	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	1	53	from	region	288:293	arg1	located					266:272	located	266:272	located	266:272	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	53	from	region	288:293	arg1	epitope					244:250	the dominant autologous neutralizing epitope	207:250	the dominant autologous neutralizing epitope in rabbits	207:261	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	4	54	theme	neutralizing	783:794	arg1	nAbs					808:811	nAbs	808:811	nAbs	808:811	We isolated autologous neutralizing antibodies (nAbs) and described their structure in complex with the B41 Env.					
31941772	4	54	theme	neutralizing	783:794	arg1	antibodies					796:805	autologous neutralizing antibodies	772:805	autologous neutralizing antibodies (nAbs)	772:812	We isolated autologous neutralizing antibodies (nAbs) and described their structure in complex with the B41 Env.					
31941772	11	55	with	complex	2084:2090	arg1	complex					2121:2127	a B41-specific antibody complex	2097:2127	a B41-specific antibody complex	2097:2127	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	11	56	theme	glycan	2183:2188	arg1	response					2195:2202	this strain-specific glycan hole response	2162:2202	this strain-specific glycan hole response	2162:2202	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	11	57	from	studies	2066:2072	arg1	complex					2084:2090	complex	2084:2090	complex with a B41-specific antibody complex	2084:2127	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	1	58	gly	glycosylated	310:321	arg1	trimer					323:328	the heavily glycosylated trimer	298:328	the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289	298:409	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	59	theme	glycoprotein	158:169	arg1	immunogens					177:186	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	9	60	theme	hole	1561:1564	arg1	antibodies					1566:1575	glycan hole antibodies	1554:1575	glycan hole antibodies	1554:1575	Our data demonstrate that the lack of cross-reactivity in glycan hole antibodies is due to amino acid differences within the epitope, and our attempts to rationally design cross-reactive trimers resulted in only limited success.					
31941772	6	61	theme	hole	1061:1064	arg1	antibodies					1066:1075	previously described BG505 glycan hole antibodies	1027:1075	previously described BG505 glycan hole antibodies	1027:1075	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	3	62	theme	disulfide	643:651	arg1	SOSIP					625:629	B41 SOSIP	621:629	B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone	621:716	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	3	62	theme	disulfide	643:651	arg1	[SOS					653:656	gp120-gp41 disulfide [SOS	632:656	gp120-gp41 disulfide [SOS	632:656	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	1	63	from	located	266:272	arg1	region					288:293	an exposed region	277:293	an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289	277:409	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	11	64	theme	microscopy	2046:2055	arg1	studies					2066:2072	Our high-resolution cryo-electron microscopy (cryoEM) studies	2012:2072	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex	2012:2127	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	10	65	dep	BG505	1966:1970	arg1	rabbits					2003:2009	trimer-immunized rabbits	1986:2009	trimer-immunized rabbits	1986:2009	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	6	66	theme	BG505	1048:1052	arg1	antibodies					1066:1075	previously described BG505 glycan hole antibodies	1027:1075	previously described BG505 glycan hole antibodies	1027:1075	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	3	67	from	[SOS	653:656	arg1	gp41					706:709	gp41	706:709	gp41	706:709	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	3	68	theme	mutation	689:696	arg1	[IP					698:700	an isoleucine-to-proline mutation [IP	664:700	an isoleucine-to-proline mutation [IP	664:700	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	12	69	theme	glycan	2250:2255	arg1	hole					2257:2260	the immunodominant glycan hole	2231:2260	the immunodominant glycan hole shared between BG505 and B41	2231:2289	We conclude that even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.					
31941772	6	70	attach	present	1153:1159	arg2	glycan					1136:1141	the >97% conserved N241 glycan	1112:1141	the >97% conserved N241 glycan	1112:1141	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	6	70	attach	present	1153:1159	arg1	B41					1164:1166	B41	1164:1166	B41	1164:1166	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	6	70	attach	present	1153:1159	arg2	present					1153:1159	present	1153:1159	present	1153:1159	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	1	71	from	epitope	244:250	arg1	rabbits					255:261	rabbits	255:261	rabbits	255:261	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	3	72	theme	broader	529:535	arg1	neutralization					537:550	broader neutralization	529:550	broader neutralization to the common glycan hole	529:576	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	1	73	gly	glycosylation	360:372	arg2	sites					374:378	potential N-linked glycosylation sites	341:378	potential N-linked glycosylation sites	341:378	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	9	74	theme	acid	1593:1596	arg1	differences					1598:1608	amino acid differences	1587:1608	amino acid differences within the epitope	1587:1627	Our data demonstrate that the lack of cross-reactivity in glycan hole antibodies is due to amino acid differences within the epitope, and our attempts to rationally design cross-reactive trimers resulted in only limited success.					
31941772	6	75	theme	conserved	1121:1129	arg1	glycan					1136:1141	the >97% conserved N241 glycan	1112:1141	the >97% conserved N241 glycan	1112:1141	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	6	75	theme	conserved	1121:1129	arg1	present					1153:1159	present	1153:1159	present	1153:1159	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	10	76	theme	glycan	1881:1886	arg1	hole					1888:1891	A glycan hole	1879:1891	A glycan hole	1879:1891	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	1	77	theme	Extensive	98:106	arg1	studies					108:114	Extensive studies	98:114	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	98:186	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	9	78	from	lack	1526:1529	arg1	antibodies					1566:1575	glycan hole antibodies	1554:1575	glycan hole antibodies	1554:1575	Our data demonstrate that the lack of cross-reactivity in glycan hole antibodies is due to amino acid differences within the epitope, and our attempts to rationally design cross-reactive trimers resulted in only limited success.					
31941772	8	79	theme	glycan	1374:1379	arg1	epitope					1386:1392	the BG505 glycan hole epitope	1364:1392	the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb	1364:1454	We then used our high-resolution data to guide mutations in the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb, but we recovered only partial binding.					
31941772	7	80	theme	glycan	1282:1287	arg1	epitopes					1294:1301	overlapping glycan hole epitopes	1270:1301	overlapping glycan hole epitopes	1270:1301	Single-particle cryo-electron microscopy studies confirmed that B41- and BG505-specific nAbs bind to overlapping glycan hole epitopes.					
31941772	3	81	theme	common	559:564	arg1	hole					573:576	the common glycan hole	555:576	the common glycan hole	555:576	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	5	82	theme	B41	953:955	arg1	immunogens					957:966	B41 immunogens	953:966	B41 immunogens	953:966	Our data suggest that distinct autologous nAb lineages are induced by BG505 and B41 immunogens, even when both were administered together.					
31941772	6	83	theme	B41-specific	1082:1093	arg1	nAbs					1095:1098	the B41-specific nAbs	1078:1098	the B41-specific nAbs	1078:1098	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	8	84	from	mutations	1351:1359	arg1	epitope					1386:1392	the BG505 glycan hole epitope	1364:1392	the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb	1364:1454	We then used our high-resolution data to guide mutations in the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb, but we recovered only partial binding.					
31941772	1	85	theme	trimer	323:328	arg1	region					288:293	an exposed region	277:293	an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289	277:409	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	86	theme	HIV	145:147	arg1	Env					172:174	Env	172:174	Env	172:174	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	86	theme	HIV	145:147	arg1	glycoprotein					158:169	A BG505-derived HIV envelope glycoprotein	129:169	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	2	87	theme	subtype	440:446	arg1	virus					450:454	a subtype B virus	438:454	a subtype B virus	438:454	The Env derived from B41, a subtype B virus, shares a glycan hole centered on positions 230 and 289.					
31941772	2	87	theme	subtype	440:446	arg1	B41					433:435	B41	433:435	B41	433:435	The Env derived from B41, a subtype B virus, shares a glycan hole centered on positions 230 and 289.					
31941772	6	88	dep	conserved	1121:1129	arg1	%					1119:1119	>97%	1116:1119	>97%	1116:1119	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	10	89	theme	autologous	1921:1930	arg1	epitopes					1945:1952	the most dominant autologous neutralizing epitopes	1903:1952	the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits	1903:2009	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	8	90	from	epitope	1386:1392	arg1	attempt					1400:1406	an attempt to broaden the reactivity of a B41-specific nAb	1397:1454	an attempt to broaden the reactivity of a B41-specific nAb	1397:1454	We then used our high-resolution data to guide mutations in the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb, but we recovered only partial binding.					
31941772	2	91	theme	glycan	466:471	arg1	hole					473:476	a glycan hole	464:476	a glycan hole centered on positions 230 and 289	464:510	The Env derived from B41, a subtype B virus, shares a glycan hole centered on positions 230 and 289.					
31941772	11	92	theme	response	2195:2202	arg1	basis					2153:2157	the molecular basis	2139:2157	the molecular basis of this strain-specific glycan hole response	2139:2202	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	1	93	theme	N-linked	351:358	arg1	sites					374:378	potential N-linked glycosylation sites	341:378	potential N-linked glycosylation sites	341:378	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	7	94	theme	B41-	1233:1236	arg1	nAbs					1257:1260	B41- and BG505-specific nAbs	1233:1260	B41- and BG505-specific nAbs	1233:1260	Single-particle cryo-electron microscopy studies confirmed that B41- and BG505-specific nAbs bind to overlapping glycan hole epitopes.					
31941772	9	95	theme	cross-reactive	1668:1681	arg1	trimers					1683:1689	cross-reactive trimers	1668:1689	cross-reactive trimers resulted in only limited success	1668:1722	Our data demonstrate that the lack of cross-reactivity in glycan hole antibodies is due to amino acid differences within the epitope, and our attempts to rationally design cross-reactive trimers resulted in only limited success.					
31941772	1	96	theme	dominant	211:218	arg1	located					266:272	located	266:272	located	266:272	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	96	theme	dominant	211:218	arg1	epitope					244:250	the dominant autologous neutralizing epitope	207:250	the dominant autologous neutralizing epitope in rabbits	207:261	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	0	97	theme	Antibody	11:18	arg1	Responses					20:28	Autologous Antibody Responses	0:28	Autologous Antibody Responses to an HIV Envelope Glycan Hole	0:59	Autologous Antibody Responses to an HIV Envelope Glycan Hole Are Not Easily Broadened in Rabbits.					
31941772	2	98	attach	derived	420:426	arg1	virus					450:454	a subtype B virus	438:454	a subtype B virus	438:454	The Env derived from B41, a subtype B virus, shares a glycan hole centered on positions 230 and 289.					
31941772	2	98	attach	derived	420:426	arg1	B41					433:435	B41	433:435	B41	433:435	The Env derived from B41, a subtype B virus, shares a glycan hole centered on positions 230 and 289.					
31941772	2	98	attach	derived	420:426	arg2	Env					416:418	The Env	412:418	The Env derived from B41, a subtype B virus,	412:455	The Env derived from B41, a subtype B virus, shares a glycan hole centered on positions 230 and 289.					
31941772	11	99	theme	B41	2077:2079	arg1	studies					2066:2072	Our high-resolution cryo-electron microscopy (cryoEM) studies	2012:2072	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex	2012:2127	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	8	100	theme	B41-specific	1439:1450	arg1	nAb					1452:1454	a B41-specific nAb	1437:1454	a B41-specific nAb	1437:1454	We then used our high-resolution data to guide mutations in the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb, but we recovered only partial binding.					
31941772	7	101	theme	hole	1289:1292	arg1	epitopes					1294:1301	overlapping glycan hole epitopes	1270:1301	overlapping glycan hole epitopes	1270:1301	Single-particle cryo-electron microscopy studies confirmed that B41- and BG505-specific nAbs bind to overlapping glycan hole epitopes.					
31941772	7	102	theme	microscopy	1199:1208	arg1	studies					1210:1216	Single-particle cryo-electron microscopy studies	1169:1216	Single-particle cryo-electron microscopy studies	1169:1216	Single-particle cryo-electron microscopy studies confirmed that B41- and BG505-specific nAbs bind to overlapping glycan hole epitopes.					
31941772	0	103	theme	HIV	36:38	arg1	Hole					56:59	an HIV Envelope Glycan Hole	33:59	an HIV Envelope Glycan Hole	33:59	Autologous Antibody Responses to an HIV Envelope Glycan Hole Are Not Easily Broadened in Rabbits.					
31941772	1	104	theme	exposed	280:286	arg1	region					288:293	an exposed region	277:293	an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289	277:409	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	5	105	theme	nAb	915:917	arg1	lineages					919:926	distinct autologous nAb lineages	895:926	distinct autologous nAb lineages	895:926	Our data suggest that distinct autologous nAb lineages are induced by BG505 and B41 immunogens, even when both were administered together.					
31941772	7	106	theme	Single-particle	1169:1183	arg1	microscopy					1199:1208	Single-particle cryo-electron microscopy	1169:1208	Single-particle cryo-electron microscopy studies	1169:1216	Single-particle cryo-electron microscopy studies confirmed that B41- and BG505-specific nAbs bind to overlapping glycan hole epitopes.					
31941772	12	107	theme	prime-boost	2318:2328	arg1	immunogens					2330:2339	prime-boost immunogens	2318:2339	prime-boost immunogens	2318:2339	We conclude that even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.					
31941772	1	108	link	BG505-derived	131:143	arg1	Env					172:174	Env	172:174	Env	172:174	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	108	link	BG505-derived	131:143	arg1	glycoprotein					158:169	A BG505-derived HIV envelope glycoprotein	129:169	subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens	121:186	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	11	109	theme	molecular	2143:2151	arg1	basis					2153:2157	the molecular basis	2139:2157	the molecular basis of this strain-specific glycan hole response	2139:2202	Our high-resolution cryo-electron microscopy (cryoEM) studies of B41 in complex with a B41-specific antibody complex elucidate the molecular basis of this strain-specific glycan hole response.					
31941772	0	110	theme	Glycan	49:54	arg1	Hole					56:59	an HIV Envelope Glycan Hole	33:59	an HIV Envelope Glycan Hole	33:59	Autologous Antibody Responses to an HIV Envelope Glycan Hole Are Not Easily Broadened in Rabbits.					
31941772	8	111	used	used	1312:1315	arg2	We					1304:1305	We	1304:1305	We	1304:1305	We then used our high-resolution data to guide mutations in the BG505 glycan hole epitope in an attempt to broaden the reactivity of a B41-specific nAb, but we recovered only partial binding.					
31941772	10	112	theme	glycan	1759:1764	arg1	hole					1766:1769	the immunodominant glycan hole	1740:1769	the immunodominant glycan hole shared between BG505 and B41	1740:1798	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	3	113	with	[SOS	653:656	arg1	[IP					698:700	an isoleucine-to-proline mutation [IP	664:700	an isoleucine-to-proline mutation [IP	664:700	To test whether broader neutralization to the common glycan hole can be achieved, we immunized rabbits with B41 SOSIP (gp120-gp41 disulfide [SOS] with an isoleucine-to-proline mutation [IP] in gp41) alone, as well as B41 and BG505 coimmunization.					
31941772	6	114	from	present	1153:1159	arg1	B41					1164:1166	B41	1164:1166	B41	1164:1166	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	5	115	theme	distinct	895:902	arg1	lineages					919:926	distinct autologous nAb lineages	895:926	distinct autologous nAb lineages	895:926	Our data suggest that distinct autologous nAb lineages are induced by BG505 and B41 immunogens, even when both were administered together.					
31941772	10	116	theme	epitopes	1945:1952	arg1	epitopes					1945:1952	the most dominant autologous neutralizing epitopes	1903:1952	the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits	1903:2009	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	10	116	theme	epitopes	1945:1952	arg1	prospect					1805:1812	the prospect	1801:1812	the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole	1801:1891	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	10	116	theme	epitopes	1945:1952	arg1	one					1896:1898	one	1896:1898	one	1896:1898	Thus, even for the immunodominant glycan hole shared between BG505 and B41, the prospect of designing prime-boost immunogens remains difficult.IMPORTANCE A glycan hole is one of the most dominant autologous neutralizing epitopes targeted on BG505 and B41 SOSIP trimer-immunized rabbits.					
31941772	1	117	theme	neutralizing	231:242	arg1	located					266:272	located	266:272	located	266:272	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	1	117	theme	neutralizing	231:242	arg1	epitope					244:250	the dominant autologous neutralizing epitope	207:250	the dominant autologous neutralizing epitope in rabbits	207:261	Extensive studies with subtype A BG505-derived HIV envelope glycoprotein (Env) immunogens have revealed that the dominant autologous neutralizing epitope in rabbits is located in an exposed region of the heavily glycosylated trimer that lacks potential N-linked glycosylation sites at positions 230, 241, and 289.					
31941772	6	118	from	B41	1164:1166	arg1	glycan					1136:1141	the >97% conserved N241 glycan	1112:1141	the >97% conserved N241 glycan	1112:1141	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
31941772	6	118	from	B41	1164:1166	arg1	present					1153:1159	present	1153:1159	present	1153:1159	In contrast to previously described BG505 glycan hole antibodies, the B41-specific nAbs accommodate the >97% conserved N241 glycan, which is present in B41.					
32005975	4	0	theme	high	568:571	arg1	expression					573:582	C1GALT1 high expression	560:582	C1GALT1 high expression in gastric adenocarcinomas	560:609	Here, we showed that C1GALT1 high expression in gastric adenocarcinomas correlated with adverse clinicopathologic features and is an independent prognostic factor for poor overall survival.					
32005975	7	1	theme	T429A	1139:1143	arg1	mutants					1145:1151	both S277A and T429A mutants	1124:1151	both S277A and T429A mutants	1124:1151	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	7	1	theme	T429A	1139:1143	arg1	O-glycosites					1164:1175	O-glycosites	1164:1175	O-glycosites on EPHA2	1164:1184	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	3	2	theme	EPH	497:499	arg1	receptors					501:509	EPH receptors	497:509	EPH receptors	497:509	However, the role of C1GALT1 in the signaling of EPH receptors remains largely overlooked.					
32005975	7	3	theme	S277A	1129:1133	arg1	mutants					1145:1151	both S277A and T429A mutants	1124:1151	both S277A and T429A mutants	1124:1151	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	7	3	theme	S277A	1129:1133	arg1	O-glycosites					1164:1175	O-glycosites	1164:1175	O-glycosites on EPHA2	1164:1184	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	2	4	theme	EPH	294:296	arg1	receptors					298:306	EPH receptors	294:306	EPH receptors	294:306	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	7	5	from	O-glycans	1076:1084	arg1	EPHA2					1089:1093	EPHA2	1089:1093	EPHA2	1089:1093	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	4	6	theme	prognostic	684:693	arg1	factor					695:700	an independent prognostic factor	669:700	an independent prognostic factor for poor overall survival	669:726	Here, we showed that C1GALT1 high expression in gastric adenocarcinomas correlated with adverse clinicopathologic features and is an independent prognostic factor for poor overall survival.					
32005975	2	7	theme	largest	321:327	arg1	family					329:334	the largest family	317:334	the largest family of receptor tyrosine kinases (RTKs)	317:370	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	1	8	theme	GalNAc-type	180:190	arg1	O-glycosylation					192:206	GalNAc-type O-glycosylation	180:206	GalNAc-type O-glycosylation	180:206	C1GALT1 controls the crucial step of GalNAc-type O-glycosylation and is associated with both physiologic and pathologic conditions, including cancers.					
32005975	4	9	theme	clinicopathologic	635:651	arg1	features					653:660	adverse clinicopathologic features	627:660	adverse clinicopathologic features	627:660	Here, we showed that C1GALT1 high expression in gastric adenocarcinomas correlated with adverse clinicopathologic features and is an independent prognostic factor for poor overall survival.					
32005975	11	10	theme	EPH	1861:1863	arg1	diseases					1884:1891	EPH receptor-regulated diseases	1861:1891	EPH receptor-regulated diseases	1861:1891	Our study highlights the importance of GalNAc-type O-glycosylation in EPH receptor-regulated diseases and identifies C1GALT1 as a potential therapeutic target for gastric cancer.					
32005975	0	11	theme	EPHA2	118:122	arg1	activation					104:113	activation	104:113	activation of EPHA2 in gastric cancer	104:140	C1GALT1 is associated with poor survival and promotes soluble Ephrin A1-mediated cell migration through activation of EPHA2 in gastric cancer.					
32005975	10	12	theme	EPHA2	1768:1772	arg1	O-glycosylation					1774:1788	EPHA2 O-glycosylation	1768:1788	EPHA2 O-glycosylation	1768:1788	These results suggest that C1GALT1 promotes phosphorylation of EPHA2 and enhances soluble Ephrin A1-mediated migration primarily by modifying EPHA2 O-glycosylation.					
32005975	9	13	theme	knockdown	1501:1509	arg1	effects					1482:1488	The effects	1478:1488	The effects of C1GALT1 knockdown or knockout on cell invasiveness in vitro and in vivo	1478:1563	The effects of C1GALT1 knockdown or knockout on cell invasiveness in vitro and in vivo were phenocopied by EPHA2 knockdown in gastric cancer cells.					
32005975	0	14	theme	gastric	127:133	arg1	cancer					135:140	gastric cancer	127:140	gastric cancer	127:140	C1GALT1 is associated with poor survival and promotes soluble Ephrin A1-mediated cell migration through activation of EPHA2 in gastric cancer.					
32005975	9	15	theme	knockout	1514:1521	arg1	effects					1482:1488	The effects	1478:1488	The effects of C1GALT1 knockdown or knockout on cell invasiveness in vitro and in vivo	1478:1563	The effects of C1GALT1 knockdown or knockout on cell invasiveness in vitro and in vivo were phenocopied by EPHA2 knockdown in gastric cancer cells.					
32005975	6	16	theme	western	946:952	arg1	analysis					959:966	Phospho-RTK array and western blot analysis	924:966	analysis	959:966	Phospho-RTK array and western blot analysis showed that C1GALT1 depletion suppressed tyrosine phosphorylation of EPHA2 induced by soluble Ephrin A1-Fc.					
32005975	9	17	theme	cell	1526:1529	arg1	invasiveness					1531:1542	cell invasiveness	1526:1542	cell invasiveness in vitro and in vivo	1526:1563	The effects of C1GALT1 knockdown or knockout on cell invasiveness in vitro and in vivo were phenocopied by EPHA2 knockdown in gastric cancer cells.					
32005975	0	18	from	activation	104:113	arg1	cancer					135:140	gastric cancer	127:140	gastric cancer	127:140	C1GALT1 is associated with poor survival and promotes soluble Ephrin A1-mediated cell migration through activation of EPHA2 in gastric cancer.					
32005975	5	19	from	cytotoxicity	869:880	arg1	AGS					903:905	AGS	903:905	AGS	903:905	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	19	from	cytotoxicity	869:880	arg1	cells					917:921	MKN45 cells	911:921	MKN45 cells	911:921	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	0	20	theme	soluble	54:60	arg1	migration					86:94	soluble Ephrin A1-mediated cell migration	54:94	soluble Ephrin A1-mediated cell migration	54:94	C1GALT1 is associated with poor survival and promotes soluble Ephrin A1-mediated cell migration through activation of EPHA2 in gastric cancer.					
32005975	5	21	from	apoptosis	855:863	arg1	AGS					903:905	AGS	903:905	AGS	903:905	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	21	from	apoptosis	855:863	arg1	cells					917:921	MKN45 cells	911:921	MKN45 cells	911:921	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	11	22	theme	potential	1921:1929	arg1	target					1943:1948	a potential therapeutic target	1919:1948	a potential therapeutic target for gastric cancer	1919:1967	Our study highlights the importance of GalNAc-type O-glycosylation in EPH receptor-regulated diseases and identifies C1GALT1 as a potential therapeutic target for gastric cancer.					
32005975	11	22	theme	potential	1921:1929	arg1	C1GALT1					1908:1914	C1GALT1	1908:1914	C1GALT1	1908:1914	Our study highlights the importance of GalNAc-type O-glycosylation in EPH receptor-regulated diseases and identifies C1GALT1 as a potential therapeutic target for gastric cancer.					
32005975	4	23	theme	overall	711:717	arg1	survival					719:726	poor overall survival	706:726	poor overall survival	706:726	Here, we showed that C1GALT1 high expression in gastric adenocarcinomas correlated with adverse clinicopathologic features and is an independent prognostic factor for poor overall survival.					
32005975	11	24	theme	O-glycosylation	1842:1856	arg1	importance					1816:1825	the importance	1812:1825	the importance of GalNAc-type O-glycosylation in EPH receptor-regulated diseases	1812:1891	Our study highlights the importance of GalNAc-type O-glycosylation in EPH receptor-regulated diseases and identifies C1GALT1 as a potential therapeutic target for gastric cancer.					
32005975	0	25	theme	A1-mediated	69:79	arg1	migration					86:94	soluble Ephrin A1-mediated cell migration	54:94	soluble Ephrin A1-mediated cell migration	54:94	C1GALT1 is associated with poor survival and promotes soluble Ephrin A1-mediated cell migration through activation of EPHA2 in gastric cancer.					
32005975	5	26	from	AGS	903:905	arg1	viability					773:781	cell viability	768:781	cell viability	768:781	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	26	from	AGS	903:905	arg1	growth					811:816	tumor growth	805:816	tumor growth	805:816	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	26	from	AGS	903:905	arg1	migration					784:792	migration	784:792	migration	784:792	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	26	from	AGS	903:905	arg1	metastasis					822:831	metastasis	822:831	metastasis	822:831	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	26	from	AGS	903:905	arg1	apoptosis					855:863	increased apoptosis	845:863	increased apoptosis	845:863	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	26	from	AGS	903:905	arg1	cytotoxicity					869:880	cytotoxicity	869:880	cytotoxicity	869:880	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	26	from	AGS	903:905	arg1	invasion					795:802	invasion	795:802	invasion	795:802	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	6	27	theme	tyrosine	1009:1016	arg1	phosphorylation					1018:1032	tyrosine phosphorylation	1009:1032	tyrosine phosphorylation of EPHA2 induced by soluble Ephrin A1-Fc	1009:1073	Phospho-RTK array and western blot analysis showed that C1GALT1 depletion suppressed tyrosine phosphorylation of EPHA2 induced by soluble Ephrin A1-Fc.					
32005975	4	28	from	expression	573:582	arg1	adenocarcinomas					595:609	gastric adenocarcinomas	587:609	gastric adenocarcinomas	587:609	Here, we showed that C1GALT1 high expression in gastric adenocarcinomas correlated with adverse clinicopathologic features and is an independent prognostic factor for poor overall survival.					
32005975	5	29	from	metastasis	822:831	arg1	AGS					903:905	AGS	903:905	AGS	903:905	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	29	from	metastasis	822:831	arg1	cells					917:921	MKN45 cells	911:921	MKN45 cells	911:921	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	30	from	5-fluorouracil	885:898	arg1	AGS					903:905	AGS	903:905	AGS	903:905	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	30	from	5-fluorouracil	885:898	arg1	cells					917:921	MKN45 cells	911:921	MKN45 cells	911:921	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	10	31	theme	Ephrin	1716:1721	arg1	migration					1735:1743	soluble Ephrin A1-mediated migration	1708:1743	soluble Ephrin A1-mediated migration	1708:1743	These results suggest that C1GALT1 promotes phosphorylation of EPHA2 and enhances soluble Ephrin A1-mediated migration primarily by modifying EPHA2 O-glycosylation.					
32005975	5	32	from	growth	811:816	arg1	AGS					903:905	AGS	903:905	AGS	903:905	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	32	from	growth	811:816	arg1	cells					917:921	MKN45 cells	911:921	MKN45 cells	911:921	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	9	33	theme	EPHA2	1585:1589	arg1	knockdown					1591:1599	EPHA2 knockdown	1585:1599	EPHA2 knockdown	1585:1599	The effects of C1GALT1 knockdown or knockout on cell invasiveness in vitro and in vivo were phenocopied by EPHA2 knockdown in gastric cancer cells.					
32005975	0	34	theme	Ephrin	62:67	arg1	migration					86:94	soluble Ephrin A1-mediated cell migration	54:94	soluble Ephrin A1-mediated cell migration	54:94	C1GALT1 is associated with poor survival and promotes soluble Ephrin A1-mediated cell migration through activation of EPHA2 in gastric cancer.					
32005975	8	35	theme	A1-Fc	1406:1410	arg1	migration					1420:1428	Ephrin A1-Fc induced migration	1399:1428	Ephrin A1-Fc induced migration	1399:1428	Furthermore, depletion of C1GALT1 decreased Ephrin A1-Fc induced migration and reduced Ephrin A1 binding to cell surfaces.					
32005975	2	36	theme	diseases	438:445	arg1	range					395:399	a diverse range	385:399	a diverse range of developmental processes and human diseases	385:445	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	10	37	theme	A1-mediated	1723:1733	arg1	migration					1735:1743	soluble Ephrin A1-mediated migration	1708:1743	soluble Ephrin A1-mediated migration	1708:1743	These results suggest that C1GALT1 promotes phosphorylation of EPHA2 and enhances soluble Ephrin A1-mediated migration primarily by modifying EPHA2 O-glycosylation.					
32005975	7	38	mod	modified	1100:1107	arg3	C1GALT1					1112:1118	C1GALT1	1112:1118	C1GALT1	1112:1118	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	7	38	mod	modified	1100:1107	arg1	O-glycans					1076:1084	O-glycans	1076:1084	O-glycans on EPHA2	1076:1093	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	9	39	theme	cancer	1612:1617	arg1	cells					1619:1623	gastric cancer cells	1604:1623	gastric cancer cells	1604:1623	The effects of C1GALT1 knockdown or knockout on cell invasiveness in vitro and in vivo were phenocopied by EPHA2 knockdown in gastric cancer cells.					
32005975	7	40	theme	EPHA2	1339:1343	arg1	activity					1345:1352	EPHA2 activity	1339:1352	EPHA2 activity	1339:1352	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	10	41	theme	soluble	1708:1714	arg1	migration					1735:1743	soluble Ephrin A1-mediated migration	1708:1743	soluble Ephrin A1-mediated migration	1708:1743	These results suggest that C1GALT1 promotes phosphorylation of EPHA2 and enhances soluble Ephrin A1-mediated migration primarily by modifying EPHA2 O-glycosylation.					
32005975	2	42	theme	developmental	404:416	arg1	processes					418:426	developmental processes	404:426	developmental processes	404:426	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	0	43	theme	poor	27:30	arg1	survival					32:39	poor survival	27:39	poor survival	27:39	C1GALT1 is associated with poor survival and promotes soluble Ephrin A1-mediated cell migration through activation of EPHA2 in gastric cancer.					
32005975	6	44	theme	soluble	1054:1060	arg1	A1-Fc					1069:1073	soluble Ephrin A1-Fc	1054:1073	soluble Ephrin A1-Fc	1054:1073	Phospho-RTK array and western blot analysis showed that C1GALT1 depletion suppressed tyrosine phosphorylation of EPHA2 induced by soluble Ephrin A1-Fc.					
32005975	8	45	theme	A1	1449:1450	arg1	binding					1452:1458	Ephrin A1 binding	1442:1458	Ephrin A1 binding to cell surfaces	1442:1475	Furthermore, depletion of C1GALT1 decreased Ephrin A1-Fc induced migration and reduced Ephrin A1 binding to cell surfaces.					
32005975	2	46	theme	kinases	357:363	arg1	family					329:334	the largest family	317:334	the largest family of receptor tyrosine kinases (RTKs)	317:370	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	5	47	theme	tumor	805:809	arg1	growth					811:816	tumor growth	805:816	tumor growth	805:816	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	7	48	theme	site-specific	1296:1308	arg1	O-glycosylation					1310:1324	site-specific O-glycosylation	1296:1324	not only overall O-glycan structures but also site-specific O-glycosylation	1250:1324	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	2	49	theme	receptor	339:346	arg1	RTKs					366:369	RTKs	366:369	RTKs	366:369	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	2	49	theme	receptor	339:346	arg1	kinases					357:363	receptor tyrosine kinases	339:363	receptor tyrosine kinases (RTKs)	339:370	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	9	50	from	effects	1482:1488	arg1	invasiveness					1531:1542	cell invasiveness	1526:1542	cell invasiveness in vitro and in vivo	1526:1563	The effects of C1GALT1 knockdown or knockout on cell invasiveness in vitro and in vivo were phenocopied by EPHA2 knockdown in gastric cancer cells.					
32005975	4	51	theme	gastric	587:593	arg1	adenocarcinomas					595:609	gastric adenocarcinomas	587:609	gastric adenocarcinomas	587:609	Here, we showed that C1GALT1 high expression in gastric adenocarcinomas correlated with adverse clinicopathologic features and is an independent prognostic factor for poor overall survival.					
32005975	3	52	theme	receptors	501:509	arg1	signaling					484:492	the signaling	480:492	the signaling of EPH receptors	480:509	However, the role of C1GALT1 in the signaling of EPH receptors remains largely overlooked.					
32005975	1	53	theme	physiologic	236:246	arg1	conditions					263:272	both physiologic and pathologic conditions	231:272	both physiologic and pathologic conditions	231:272	C1GALT1 controls the crucial step of GalNAc-type O-glycosylation and is associated with both physiologic and pathologic conditions, including cancers.					
32005975	1	53	theme	physiologic	236:246	arg1	cancers					285:291	cancers	285:291	cancers	285:291	C1GALT1 controls the crucial step of GalNAc-type O-glycosylation and is associated with both physiologic and pathologic conditions, including cancers.					
32005975	6	54	theme	Phospho-RTK	924:934	arg1	array					936:940	Phospho-RTK array and western blot analysis	924:966	array	936:940	Phospho-RTK array and western blot analysis showed that C1GALT1 depletion suppressed tyrosine phosphorylation of EPHA2 induced by soluble Ephrin A1-Fc.					
32005975	7	55	theme	O-glycan	1267:1274	arg1	structures					1276:1285	overall O-glycan structures	1259:1285	not only overall O-glycan structures but also site-specific O-glycosylation	1250:1324	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	7	56	theme	Y588	1228:1231	arg1	phosphorylation					1209:1223	phosphorylation	1209:1223	phosphorylation of Y588	1209:1231	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	11	57	theme	gastric	1954:1960	arg1	cancer					1962:1967	gastric cancer	1954:1967	gastric cancer	1954:1967	Our study highlights the importance of GalNAc-type O-glycosylation in EPH receptor-regulated diseases and identifies C1GALT1 as a potential therapeutic target for gastric cancer.					
32005975	1	58	theme	pathologic	252:261	arg1	conditions					263:272	both physiologic and pathologic conditions	231:272	both physiologic and pathologic conditions	231:272	C1GALT1 controls the crucial step of GalNAc-type O-glycosylation and is associated with both physiologic and pathologic conditions, including cancers.					
32005975	1	58	theme	pathologic	252:261	arg1	cancers					285:291	cancers	285:291	cancers	285:291	C1GALT1 controls the crucial step of GalNAc-type O-glycosylation and is associated with both physiologic and pathologic conditions, including cancers.					
32005975	4	59	theme	C1GALT1	560:566	arg1	expression					573:582	C1GALT1 high expression	560:582	C1GALT1 high expression in gastric adenocarcinomas	560:609	Here, we showed that C1GALT1 high expression in gastric adenocarcinomas correlated with adverse clinicopathologic features and is an independent prognostic factor for poor overall survival.					
32005975	1	60	theme	crucial	164:170	arg1	step					172:175	the crucial step	160:175	the crucial step of GalNAc-type O-glycosylation	160:206	C1GALT1 controls the crucial step of GalNAc-type O-glycosylation and is associated with both physiologic and pathologic conditions, including cancers.					
32005975	6	61	theme	C1GALT1	980:986	arg1	depletion					988:996	C1GALT1 depletion	980:996	C1GALT1 depletion	980:996	Phospho-RTK array and western blot analysis showed that C1GALT1 depletion suppressed tyrosine phosphorylation of EPHA2 induced by soluble Ephrin A1-Fc.					
32005975	4	62	theme	independent	672:682	arg1	factor					695:700	an independent prognostic factor	669:700	an independent prognostic factor for poor overall survival	669:726	Here, we showed that C1GALT1 high expression in gastric adenocarcinomas correlated with adverse clinicopathologic features and is an independent prognostic factor for poor overall survival.					
32005975	9	63	theme	C1GALT1	1493:1499	arg1	knockdown					1501:1509	C1GALT1 knockdown	1493:1509	C1GALT1 knockdown	1493:1509	The effects of C1GALT1 knockdown or knockout on cell invasiveness in vitro and in vivo were phenocopied by EPHA2 knockdown in gastric cancer cells.					
32005975	4	64	theme	adverse	627:633	arg1	features					653:660	adverse clinicopathologic features	627:660	adverse clinicopathologic features	627:660	Here, we showed that C1GALT1 high expression in gastric adenocarcinomas correlated with adverse clinicopathologic features and is an independent prognostic factor for poor overall survival.					
32005975	11	65	theme	receptor-regulated	1865:1882	arg1	diseases					1884:1891	EPH receptor-regulated diseases	1861:1891	EPH receptor-regulated diseases	1861:1891	Our study highlights the importance of GalNAc-type O-glycosylation in EPH receptor-regulated diseases and identifies C1GALT1 as a potential therapeutic target for gastric cancer.					
32005975	6	66	theme	blot	954:957	arg1	analysis					959:966	Phospho-RTK array and western blot analysis	924:966	analysis	959:966	Phospho-RTK array and western blot analysis showed that C1GALT1 depletion suppressed tyrosine phosphorylation of EPHA2 induced by soluble Ephrin A1-Fc.					
32005975	5	67	theme	MKN45	911:915	arg1	cells					917:921	MKN45 cells	911:921	MKN45 cells	911:921	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	68	from	migration	784:792	arg1	AGS					903:905	AGS	903:905	AGS	903:905	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	68	from	migration	784:792	arg1	cells					917:921	MKN45 cells	911:921	MKN45 cells	911:921	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	11	69	from	importance	1816:1825	arg1	diseases					1884:1891	EPH receptor-regulated diseases	1861:1891	EPH receptor-regulated diseases	1861:1891	Our study highlights the importance of GalNAc-type O-glycosylation in EPH receptor-regulated diseases and identifies C1GALT1 as a potential therapeutic target for gastric cancer.					
32005975	3	70	theme	C1GALT1	469:475	arg1	role					461:464	the role	457:464	the role of C1GALT1 in the signaling of EPH receptors	457:509	However, the role of C1GALT1 in the signaling of EPH receptors remains largely overlooked.					
32005975	5	71	theme	cell	768:771	arg1	viability					773:781	cell viability	768:781	cell viability	768:781	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	72	from	viability	773:781	arg1	AGS					903:905	AGS	903:905	AGS	903:905	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	72	from	viability	773:781	arg1	cells					917:921	MKN45 cells	911:921	MKN45 cells	911:921	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	1	73	theme	O-glycosylation	192:206	arg1	step					172:175	the crucial step	160:175	the crucial step of GalNAc-type O-glycosylation	160:206	C1GALT1 controls the crucial step of GalNAc-type O-glycosylation and is associated with both physiologic and pathologic conditions, including cancers.					
32005975	5	74	theme	5-fluorouracil	885:898	arg1	viability					773:781	cell viability	768:781	cell viability	768:781	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	74	theme	5-fluorouracil	885:898	arg1	growth					811:816	tumor growth	805:816	tumor growth	805:816	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	74	theme	5-fluorouracil	885:898	arg1	migration					784:792	migration	784:792	migration	784:792	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	74	theme	5-fluorouracil	885:898	arg1	metastasis					822:831	metastasis	822:831	metastasis	822:831	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	74	theme	5-fluorouracil	885:898	arg1	apoptosis					855:863	increased apoptosis	845:863	increased apoptosis	845:863	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	74	theme	5-fluorouracil	885:898	arg1	cytotoxicity					869:880	cytotoxicity	869:880	cytotoxicity	869:880	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	74	theme	5-fluorouracil	885:898	arg1	invasion					795:802	invasion	795:802	invasion	795:802	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	6	75	theme	EPHA2	1037:1041	arg1	phosphorylation					1018:1032	tyrosine phosphorylation	1009:1032	tyrosine phosphorylation of EPHA2 induced by soluble Ephrin A1-Fc	1009:1073	Phospho-RTK array and western blot analysis showed that C1GALT1 depletion suppressed tyrosine phosphorylation of EPHA2 induced by soluble Ephrin A1-Fc.					
32005975	0	76	theme	cell	81:84	arg1	migration					86:94	soluble Ephrin A1-mediated cell migration	54:94	soluble Ephrin A1-mediated cell migration	54:94	C1GALT1 is associated with poor survival and promotes soluble Ephrin A1-mediated cell migration through activation of EPHA2 in gastric cancer.					
32005975	4	77	theme	poor	706:709	arg1	survival					719:726	poor overall survival	706:726	poor overall survival	706:726	Here, we showed that C1GALT1 high expression in gastric adenocarcinomas correlated with adverse clinicopathologic features and is an independent prognostic factor for poor overall survival.					
32005975	5	78	from	invasion	795:802	arg1	AGS					903:905	AGS	903:905	AGS	903:905	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	78	from	invasion	795:802	arg1	cells					917:921	MKN45 cells	911:921	MKN45 cells	911:921	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	11	79	dep	potential	1921:1929	arg1	therapeutic					1931:1941	therapeutic	1931:1941	therapeutic	1931:1941	Our study highlights the importance of GalNAc-type O-glycosylation in EPH receptor-regulated diseases and identifies C1GALT1 as a potential therapeutic target for gastric cancer.					
32005975	7	80	from	O-glycosites	1164:1175	arg1	EPHA2					1180:1184	EPHA2	1180:1184	EPHA2	1180:1184	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	11	81	theme	GalNAc-type	1830:1840	arg1	O-glycosylation					1842:1856	GalNAc-type O-glycosylation	1830:1856	GalNAc-type O-glycosylation	1830:1856	Our study highlights the importance of GalNAc-type O-glycosylation in EPH receptor-regulated diseases and identifies C1GALT1 as a potential therapeutic target for gastric cancer.					
32005975	5	82	theme	C1GALT1	750:756	arg1	loss					742:745	loss	742:745	loss	742:745	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	82	theme	C1GALT1	750:756	arg1	Silencing					729:737	Silencing	729:737	Silencing	729:737	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	8	83	theme	C1GALT1	1381:1387	arg1	depletion					1368:1376	depletion	1368:1376	depletion of C1GALT1	1368:1387	Furthermore, depletion of C1GALT1 decreased Ephrin A1-Fc induced migration and reduced Ephrin A1 binding to cell surfaces.					
32005975	2	84	theme	diverse	387:393	arg1	range					395:399	a diverse range	385:399	a diverse range of developmental processes and human diseases	385:445	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	8	85	theme	Ephrin	1399:1404	arg1	migration					1420:1428	Ephrin A1-Fc induced migration	1399:1428	Ephrin A1-Fc induced migration	1399:1428	Furthermore, depletion of C1GALT1 decreased Ephrin A1-Fc induced migration and reduced Ephrin A1 binding to cell surfaces.					
32005975	5	86	from	cells	917:921	arg1	viability					773:781	cell viability	768:781	cell viability	768:781	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	86	from	cells	917:921	arg1	growth					811:816	tumor growth	805:816	tumor growth	805:816	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	86	from	cells	917:921	arg1	migration					784:792	migration	784:792	migration	784:792	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	86	from	cells	917:921	arg1	metastasis					822:831	metastasis	822:831	metastasis	822:831	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	86	from	cells	917:921	arg1	apoptosis					855:863	increased apoptosis	845:863	increased apoptosis	845:863	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	86	from	cells	917:921	arg1	cytotoxicity					869:880	cytotoxicity	869:880	cytotoxicity	869:880	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	5	86	from	cells	917:921	arg1	invasion					795:802	invasion	795:802	invasion	795:802	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	9	87	theme	gastric	1604:1610	arg1	cells					1619:1623	gastric cancer cells	1604:1623	gastric cancer cells	1604:1623	The effects of C1GALT1 knockdown or knockout on cell invasiveness in vitro and in vivo were phenocopied by EPHA2 knockdown in gastric cancer cells.					
32005975	2	88	theme	human	432:436	arg1	diseases					438:445	human diseases	432:445	human diseases	432:445	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	8	89	theme	induced	1412:1418	arg1	migration					1420:1428	Ephrin A1-Fc induced migration	1399:1428	Ephrin A1-Fc induced migration	1399:1428	Furthermore, depletion of C1GALT1 decreased Ephrin A1-Fc induced migration and reduced Ephrin A1 binding to cell surfaces.					
32005975	2	90	theme	processes	418:426	arg1	range					395:399	a diverse range	385:399	a diverse range of developmental processes and human diseases	385:445	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	6	91	theme	Ephrin	1062:1067	arg1	A1-Fc					1069:1073	soluble Ephrin A1-Fc	1054:1073	soluble Ephrin A1-Fc	1054:1073	Phospho-RTK array and western blot analysis showed that C1GALT1 depletion suppressed tyrosine phosphorylation of EPHA2 induced by soluble Ephrin A1-Fc.					
32005975	8	92	theme	Ephrin	1442:1447	arg1	binding					1452:1458	Ephrin A1 binding	1442:1458	Ephrin A1 binding to cell surfaces	1442:1475	Furthermore, depletion of C1GALT1 decreased Ephrin A1-Fc induced migration and reduced Ephrin A1 binding to cell surfaces.					
32005975	3	93	from	role	461:464	arg1	signaling					484:492	the signaling	480:492	the signaling of EPH receptors	480:509	However, the role of C1GALT1 in the signaling of EPH receptors remains largely overlooked.					
32005975	2	94	theme	tyrosine	348:355	arg1	RTKs					366:369	RTKs	366:369	RTKs	366:369	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	2	94	theme	tyrosine	348:355	arg1	kinases					357:363	receptor tyrosine kinases	339:363	receptor tyrosine kinases (RTKs)	339:370	EPH receptors comprise the largest family of receptor tyrosine kinases (RTKs) and modulate a diverse range of developmental processes and human diseases.					
32005975	7	95	gly	O-glycosites	1164:1175	arg2	mutants					1145:1151	both S277A and T429A mutants	1124:1151	both S277A and T429A mutants	1124:1151	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	7	95	gly	O-glycosites	1164:1175	arg2	O-glycosites					1164:1175	O-glycosites	1164:1175	O-glycosites on EPHA2	1164:1184	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	5	96	theme	increased	845:853	arg1	apoptosis					855:863	increased apoptosis	845:863	increased apoptosis	845:863	Silencing or loss of C1GALT1 inhibited cell viability, migration, invasion, tumor growth and metastasis, as well as increased apoptosis and cytotoxicity of 5-fluorouracil in AGS and MKN45 cells.					
32005975	7	97	theme	overall	1259:1265	arg1	structures					1276:1285	overall O-glycan structures	1259:1285	not only overall O-glycan structures but also site-specific O-glycosylation	1250:1324	O-glycans on EPHA2 were modified by C1GALT1 and both S277A and T429A mutants, which are O-glycosites on EPHA2, dramatically enhanced phosphorylation of Y588, suggesting that not only overall O-glycan structures but also site-specific O-glycosylation can regulate EPHA2 activity.					
32005975	10	98	theme	EPHA2	1689:1693	arg1	phosphorylation					1670:1684	phosphorylation	1670:1684	phosphorylation of EPHA2	1670:1693	These results suggest that C1GALT1 promotes phosphorylation of EPHA2 and enhances soluble Ephrin A1-mediated migration primarily by modifying EPHA2 O-glycosylation.					
32005975	8	99	theme	cell	1463:1466	arg1	surfaces					1468:1475	cell surfaces	1463:1475	cell surfaces	1463:1475	Furthermore, depletion of C1GALT1 decreased Ephrin A1-Fc induced migration and reduced Ephrin A1 binding to cell surfaces.					
34155258	6	0	theme	galactosylated	1135:1148	arg1	N-glycans					1163:1171	more than 80% galactosylated bi-antennary N-glycans	1121:1171	more than 80% galactosylated bi-antennary N-glycans	1121:1171	Overexpressing B4GalT1 gene alone in the CHO cells produced antibodies with more than 80% galactosylated bi-antennary N-glycans.					
34155258	7	1	theme	sialylated	1271:1280	arg1	N-glycans					1295:1303	more than 70% sialylated bi-antennary N-glycans	1257:1303	more than 70% sialylated bi-antennary N-glycans	1257:1303	Combinatorial overexpression of B4GalT1 and ST6Gal1 produced antibodies containing more than 70% sialylated bi-antennary N-glycans.					
34155258	5	2	theme	multiple	1020:1027	arg1	genes					1038:1042	single or multiple critical genes	1010:1042	single or multiple critical genes	1010:1042	The bottlenecks in the N-glycosylation pathway were identified and then released by overexpressing single or multiple critical genes.					
34155258	7	3	theme	bi-antennary	1282:1293	arg1	N-glycans					1295:1303	more than 70% sialylated bi-antennary N-glycans	1257:1303	more than 70% sialylated bi-antennary N-glycans	1257:1303	Combinatorial overexpression of B4GalT1 and ST6Gal1 produced antibodies containing more than 70% sialylated bi-antennary N-glycans.					
34155258	1	4	theme	complex-type	192:203	arg1	N-glycans					205:213	complex-type N-glycans	192:213	complex-type N-glycans that significantly affect their biodistribution and bioactivity	192:277	Therapeutic antibodies are decorated with complex-type N-glycans that significantly affect their biodistribution and bioactivity.					
34155258	9	5	theme	therapeutic	1684:1694	arg1	applications					1696:1707	different therapeutic applications	1674:1707	different therapeutic applications	1674:1707	The various N-glycan structures and the method for producing them in this work provide opportunities to study the glycan structure-and-function and develop novel recombinant antibodies for addressing different therapeutic applications.					
34155258	3	6	theme	N-glycan	424:431	arg1	processing					433:442	N-glycan processing	424:442	N-glycan processing	424:442	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	4	7	theme	N-linked	887:894	arg1	glycosylation					896:908	antibody N-linked glycosylation	878:908	antibody N-linked glycosylation	878:908	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	2	8	theme	CHO	358:360	arg1	cells					362:366	wild-type CHO cells	348:366	wild-type CHO cells	348:366	The N-glycan structures on antibodies are incompletely processed in wild-type CHO cells due to their limited glycosylation capacity.					
34155258	9	9	theme	glycan	1588:1593	arg1	structure-and-function					1595:1616	the glycan structure-and-function	1584:1616	the glycan structure-and-function	1584:1616	The various N-glycan structures and the method for producing them in this work provide opportunities to study the glycan structure-and-function and develop novel recombinant antibodies for addressing different therapeutic applications.					
34155258	4	10	theme	genes	846:850	arg1	panel					808:812	a panel	806:812	a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation	806:908	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	6	11	dep	galactosylated	1135:1148	arg1	%					1133:1133	more than 80%	1121:1133	more than 80%	1121:1133	Overexpressing B4GalT1 gene alone in the CHO cells produced antibodies with more than 80% galactosylated bi-antennary N-glycans.					
34155258	3	12	theme	clonal	637:642	arg1	variations					644:653	large clonal variations	631:653	large clonal variations in gene expression levels	631:679	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	4	13	from	impact	868:873	arg1	glycosylation					896:908	antibody N-linked glycosylation	878:908	antibody N-linked glycosylation	878:908	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	2	14	theme	wild-type	348:356	arg1	cells					362:366	wild-type CHO cells	348:366	wild-type CHO cells	348:366	The N-glycan structures on antibodies are incompletely processed in wild-type CHO cells due to their limited glycosylation capacity.					
34155258	0	15	theme	complex-type	126:137	arg1	N-glycans					139:147	diverse complex-type N-glycans	118:147	diverse complex-type N-glycans	118:147	Multiplexed engineering glycosyltransferase genes in CHO cells via targeted integration for producing antibodies with diverse complex-type N-glycans.					
34155258	8	16	with	combination	1436:1446	arg1	ST6Gal1					1465:1471	ST6Gal1	1465:1471	ST6Gal1	1465:1471	In addition, antibodies with various tri-antennary N-glycans were obtained for the first time by overexpressing MGAT5 alone or in combination with B4GalT1 and ST6Gal1.					
34155258	8	16	with	combination	1436:1446	arg1	B4GalT1					1453:1459	B4GalT1	1453:1459	B4GalT1	1453:1459	In addition, antibodies with various tri-antennary N-glycans were obtained for the first time by overexpressing MGAT5 alone or in combination with B4GalT1 and ST6Gal1.					
34155258	7	17	dep	N-glycans	1295:1303	arg1	%					1269:1269	%	1269:1269	%	1269:1269	Combinatorial overexpression of B4GalT1 and ST6Gal1 produced antibodies containing more than 70% sialylated bi-antennary N-glycans.					
34155258	4	18	theme	antibody	878:885	arg1	glycosylation					896:908	antibody N-linked glycosylation	878:908	antibody N-linked glycosylation	878:908	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	9	19	theme	novel	1630:1634	arg1	antibodies					1648:1657	novel recombinant antibodies	1630:1657	novel recombinant antibodies for addressing different therapeutic applications	1630:1707	The various N-glycan structures and the method for producing them in this work provide opportunities to study the glycan structure-and-function and develop novel recombinant antibodies for addressing different therapeutic applications.					
34155258	0	20	theme	diverse	118:124	arg1	N-glycans					139:147	diverse complex-type N-glycans	118:147	diverse complex-type N-glycans	118:147	Multiplexed engineering glycosyltransferase genes in CHO cells via targeted integration for producing antibodies with diverse complex-type N-glycans.					
34155258	5	21	from	bottlenecks	915:925	arg1	pathway					950:956	the N-glycosylation pathway	930:956	the N-glycosylation pathway	930:956	The bottlenecks in the N-glycosylation pathway were identified and then released by overexpressing single or multiple critical genes.					
34155258	9	22	from	method	1514:1519	arg1	work					1548:1551	this work	1543:1551	this work	1543:1551	The various N-glycan structures and the method for producing them in this work provide opportunities to study the glycan structure-and-function and develop novel recombinant antibodies for addressing different therapeutic applications.					
34155258	6	23	theme	bi-antennary	1150:1161	arg1	N-glycans					1163:1171	more than 80% galactosylated bi-antennary N-glycans	1121:1171	more than 80% galactosylated bi-antennary N-glycans	1121:1171	Overexpressing B4GalT1 gene alone in the CHO cells produced antibodies with more than 80% galactosylated bi-antennary N-glycans.					
34155258	2	24	from	structures	293:302	arg1	antibodies					307:316	antibodies	307:316	antibodies	307:316	The N-glycan structures on antibodies are incompletely processed in wild-type CHO cells due to their limited glycosylation capacity.					
34155258	4	25	theme	recombinase-mediated-cassette-exchange	734:771	arg1	technology					780:789	recombinase-mediated-cassette-exchange (RMCE) technology	734:789	recombinase-mediated-cassette-exchange (RMCE) technology	734:789	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	8	26	theme	tri-antennary	1343:1355	arg1	N-glycans					1357:1365	various tri-antennary N-glycans	1335:1365	various tri-antennary N-glycans	1335:1365	In addition, antibodies with various tri-antennary N-glycans were obtained for the first time by overexpressing MGAT5 alone or in combination with B4GalT1 and ST6Gal1.					
34155258	3	27	theme	gene	658:661	arg1	levels					674:679	gene expression levels	658:679	gene expression levels	658:679	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	9	28	theme	various	1478:1484	arg1	structures					1495:1504	various N-glycan structures	1478:1504	various N-glycan structures	1478:1504	The various N-glycan structures and the method for producing them in this work provide opportunities to study the glycan structure-and-function and develop novel recombinant antibodies for addressing different therapeutic applications.					
34155258	3	29	theme	CHO	512:514	arg1	cells					516:520	CHO cells	512:520	CHO cells	512:520	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	7	30	dep	%	1269:1269	arg1	70					1267:1268	70	1267:1268	70	1267:1268	Combinatorial overexpression of B4GalT1 and ST6Gal1 produced antibodies containing more than 70% sialylated bi-antennary N-glycans.					
34155258	8	31	with	antibodies	1319:1328	arg1	N-glycans					1357:1365	various tri-antennary N-glycans	1335:1365	various tri-antennary N-glycans	1335:1365	In addition, antibodies with various tri-antennary N-glycans were obtained for the first time by overexpressing MGAT5 alone or in combination with B4GalT1 and ST6Gal1.					
34155258	3	32	theme	expression	663:672	arg1	levels					674:679	gene expression levels	658:679	gene expression levels	658:679	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	4	33	theme	RMCE	774:777	arg1	technology					780:789	recombinase-mediated-cassette-exchange (RMCE) technology	734:789	recombinase-mediated-cassette-exchange (RMCE) technology	734:789	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	4	34	theme	glycosyltransferase	826:844	arg1	genes					846:850	42 human glycosyltransferase genes	817:850	42 human glycosyltransferase genes	817:850	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	7	35	theme	ST6Gal1	1218:1224	arg1	overexpression					1188:1201	Combinatorial overexpression	1174:1201	Combinatorial overexpression of B4GalT1 and ST6Gal1	1174:1224	Combinatorial overexpression of B4GalT1 and ST6Gal1 produced antibodies containing more than 70% sialylated bi-antennary N-glycans.					
34155258	0	36	with	antibodies	102:111	arg1	N-glycans					139:147	diverse complex-type N-glycans	118:147	diverse complex-type N-glycans	118:147	Multiplexed engineering glycosyltransferase genes in CHO cells via targeted integration for producing antibodies with diverse complex-type N-glycans.					
34155258	3	37	theme	N-glycosylation	547:561	arg1	pathway					563:569	the cellular N-glycosylation pathway	534:569	the cellular N-glycosylation pathway	534:569	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	3	38	theme	glycosyltransferase	445:463	arg1	genes					465:469	glycosyltransferase genes	445:469	glycosyltransferase genes	445:469	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	6	39	with	antibodies	1105:1114	arg1	N-glycans					1163:1171	more than 80% galactosylated bi-antennary N-glycans	1121:1171	more than 80% galactosylated bi-antennary N-glycans	1121:1171	Overexpressing B4GalT1 gene alone in the CHO cells produced antibodies with more than 80% galactosylated bi-antennary N-glycans.					
34155258	5	40	theme	critical	1029:1036	arg1	genes					1038:1042	single or multiple critical genes	1010:1042	single or multiple critical genes	1010:1042	The bottlenecks in the N-glycosylation pathway were identified and then released by overexpressing single or multiple critical genes.					
34155258	7	41	gly	sialylated	1271:1280	arg1	N-glycans					1295:1303	more than 70% sialylated bi-antennary N-glycans	1257:1303	more than 70% sialylated bi-antennary N-glycans	1257:1303	Combinatorial overexpression of B4GalT1 and ST6Gal1 produced antibodies containing more than 70% sialylated bi-antennary N-glycans.					
34155258	0	42	theme	CHO	53:55	arg1	cells					57:61	CHO cells	53:61	CHO cells	53:61	Multiplexed engineering glycosyltransferase genes in CHO cells via targeted integration for producing antibodies with diverse complex-type N-glycans.					
34155258	6	43	theme	CHO	1086:1088	arg1	cells					1090:1094	the CHO cells	1082:1094	the CHO cells	1082:1094	Overexpressing B4GalT1 gene alone in the CHO cells produced antibodies with more than 80% galactosylated bi-antennary N-glycans.					
34155258	7	44	theme	B4GalT1	1206:1212	arg1	overexpression					1188:1201	Combinatorial overexpression	1174:1201	Combinatorial overexpression of B4GalT1 and ST6Gal1	1174:1224	Combinatorial overexpression of B4GalT1 and ST6Gal1 produced antibodies containing more than 70% sialylated bi-antennary N-glycans.					
34155258	9	45	theme	recombinant	1636:1646	arg1	antibodies					1648:1657	novel recombinant antibodies	1630:1657	novel recombinant antibodies for addressing different therapeutic applications	1630:1707	The various N-glycan structures and the method for producing them in this work provide opportunities to study the glycan structure-and-function and develop novel recombinant antibodies for addressing different therapeutic applications.					
34155258	7	46	contain	containing	1246:1255	arg2	N-glycans					1295:1303	more than 70% sialylated bi-antennary N-glycans	1257:1303	more than 70% sialylated bi-antennary N-glycans	1257:1303	Combinatorial overexpression of B4GalT1 and ST6Gal1 produced antibodies containing more than 70% sialylated bi-antennary N-glycans.					
34155258	7	46	contain	containing	1246:1255	arg1	antibodies					1235:1244	antibodies	1235:1244	antibodies containing more than 70% sialylated bi-antennary N-glycans	1235:1303	Combinatorial overexpression of B4GalT1 and ST6Gal1 produced antibodies containing more than 70% sialylated bi-antennary N-glycans.					
34155258	2	47	theme	N-glycan	284:291	arg1	structures					293:302	The N-glycan structures	280:302	The N-glycan structures on antibodies	280:316	The N-glycan structures on antibodies are incompletely processed in wild-type CHO cells due to their limited glycosylation capacity.					
34155258	3	48	theme	cellular	538:545	arg1	pathway					563:569	the cellular N-glycosylation pathway	534:569	the cellular N-glycosylation pathway	534:569	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	9	49	dep	structures	1495:1504	arg1	The					1474:1476	The	1474:1476	The	1474:1476	The various N-glycan structures and the method for producing them in this work provide opportunities to study the glycan structure-and-function and develop novel recombinant antibodies for addressing different therapeutic applications.					
34155258	5	50	theme	N-glycosylation	934:948	arg1	pathway					950:956	the N-glycosylation pathway	930:956	the N-glycosylation pathway	930:956	The bottlenecks in the N-glycosylation pathway were identified and then released by overexpressing single or multiple critical genes.					
34155258	9	51	theme	N-glycan	1486:1493	arg1	structures					1495:1504	various N-glycan structures	1478:1504	various N-glycan structures	1478:1504	The various N-glycan structures and the method for producing them in this work provide opportunities to study the glycan structure-and-function and develop novel recombinant antibodies for addressing different therapeutic applications.					
34155258	3	52	theme	random	580:585	arg1	integration					587:597	random integration	580:597	random integration	580:597	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	8	53	theme	first	1389:1393	arg1	time					1395:1398	the first time	1385:1398	the first time	1385:1398	In addition, antibodies with various tri-antennary N-glycans were obtained for the first time by overexpressing MGAT5 alone or in combination with B4GalT1 and ST6Gal1.					
34155258	9	54	from	structures	1495:1504	arg1	work					1548:1551	this work	1543:1551	this work	1543:1551	The various N-glycan structures and the method for producing them in this work provide opportunities to study the glycan structure-and-function and develop novel recombinant antibodies for addressing different therapeutic applications.					
34155258	4	55	theme	human	820:824	arg1	genes					846:850	42 human glycosyltransferase genes	817:850	42 human glycosyltransferase genes	817:850	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	3	56	from	variations	644:653	arg1	levels					674:679	gene expression levels	658:679	gene expression levels	658:679	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	1	57	theme	Therapeutic	150:160	arg1	antibodies					162:171	Therapeutic antibodies	150:171	Therapeutic antibodies	150:171	Therapeutic antibodies are decorated with complex-type N-glycans that significantly affect their biodistribution and bioactivity.					
34155258	7	58	theme	Combinatorial	1174:1186	arg1	overexpression					1188:1201	Combinatorial overexpression	1174:1201	Combinatorial overexpression of B4GalT1 and ST6Gal1	1174:1224	Combinatorial overexpression of B4GalT1 and ST6Gal1 produced antibodies containing more than 70% sialylated bi-antennary N-glycans.					
34155258	8	59	theme	various	1335:1341	arg1	N-glycans					1357:1365	various tri-antennary N-glycans	1335:1365	various tri-antennary N-glycans	1335:1365	In addition, antibodies with various tri-antennary N-glycans were obtained for the first time by overexpressing MGAT5 alone or in combination with B4GalT1 and ST6Gal1.					
34155258	3	60	theme	large	631:635	arg1	variations					644:653	large clonal variations	631:653	large clonal variations in gene expression levels	631:679	To improve N-glycan processing, glycosyltransferase genes have been traditionally overexpressed in CHO cells to engineer the cellular N-glycosylation pathway by using random integration, which is often associated with large clonal variations in gene expression levels.					
34155258	2	61	theme	limited	381:387	arg1	capacity					403:410	their limited glycosylation capacity	375:410	their limited glycosylation capacity	375:410	The N-glycan structures on antibodies are incompletely processed in wild-type CHO cells due to their limited glycosylation capacity.					
34155258	2	62	theme	glycosylation	389:401	arg1	capacity					403:410	their limited glycosylation capacity	375:410	their limited glycosylation capacity	375:410	The N-glycan structures on antibodies are incompletely processed in wild-type CHO cells due to their limited glycosylation capacity.					
34155258	9	63	theme	different	1674:1682	arg1	applications					1696:1707	different therapeutic applications	1674:1707	different therapeutic applications	1674:1707	The various N-glycan structures and the method for producing them in this work provide opportunities to study the glycan structure-and-function and develop novel recombinant antibodies for addressing different therapeutic applications.					
34155258	4	64	link	N-linked	887:894	arg1	glycosylation					896:908	antibody N-linked glycosylation	878:908	antibody N-linked glycosylation	878:908	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	4	65	used	used	729:732	arg2	we					726:727	we	726:727	we	726:727	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	5	66	theme	single	1010:1015	arg1	genes					1038:1042	single or multiple critical genes	1010:1042	single or multiple critical genes	1010:1042	The bottlenecks in the N-glycosylation pathway were identified and then released by overexpressing single or multiple critical genes.					
34155258	4	67	theme	clonal	707:712	arg1	variations					714:723	the clonal variations	703:723	the clonal variations	703:723	In order to minimize the clonal variations, we used recombinase-mediated-cassette-exchange (RMCE) technology to overexpress a panel of 42 human glycosyltransferase genes to screen their impact on antibody N-linked glycosylation.					
34155258	0	68	theme	targeted	67:74	arg1	integration					76:86	targeted integration	67:86	targeted integration for producing antibodies with diverse complex-type N-glycans	67:147	Multiplexed engineering glycosyltransferase genes in CHO cells via targeted integration for producing antibodies with diverse complex-type N-glycans.					
34155258	6	69	theme	B4GalT1	1060:1066	arg1	gene					1068:1071	B4GalT1 gene	1060:1071	B4GalT1 gene alone	1060:1077	Overexpressing B4GalT1 gene alone in the CHO cells produced antibodies with more than 80% galactosylated bi-antennary N-glycans.					
34155258	0	70	theme	glycosyltransferase	24:42	arg1	genes					44:48	glycosyltransferase genes	24:48	glycosyltransferase genes	24:48	Multiplexed engineering glycosyltransferase genes in CHO cells via targeted integration for producing antibodies with diverse complex-type N-glycans.					
33118822	9	0	theme	quality	1860:1866	arg1	control					1868:1874	quality control	1860:1874	quality control	1860:1874	Overall, our integration strategy can be easily implemented for in-depth mAb structural heterogeneity characterization during pharmaceutical development and quality control.					
33118822	5	1	theme	LC	1173:1174	arg1	run					1176:1178	a single LC run	1164:1178	a single LC run	1164:1178	Based on collision-induced dissociation and ETD-combined middle-down analysis, sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits, respectively, were reached in a single LC run.					
33118822	7	2	theme	glycosylation	1466:1478	arg1	variants					1480:1487	glycosylation variants	1466:1487	glycosylation variants	1466:1487	We finally integrated the native MS and middle-down results to have a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab.					
33118822	1	3	theme	monoclonal	205:214	arg1	mAb					226:228	mAb	226:228	mAb	226:228	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is critical for drug development but remains challenging due to the inherent structural heterogeneity.					
33118822	1	3	theme	monoclonal	205:214	arg1	antibody					216:223	therapeutic monoclonal antibody	193:223	therapeutic monoclonal antibody (mAb) structures	193:240	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is critical for drug development but remains challenging due to the inherent structural heterogeneity.					
33118822	7	4	theme	variants	1452:1459	arg1	detection					1412:1420	a more realistic detection	1395:1420	a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab	1395:1502	We finally integrated the native MS and middle-down results to have a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab.					
33118822	2	5	theme	complementary	542:554	arg1	information					556:566	complementary information	542:566	complementary information obtained from intact mass and middle-down analyses	542:617	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	7	6	theme	trastuzumab	1492:1502	arg1	detection					1412:1420	a more realistic detection	1395:1420	a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab	1395:1502	We finally integrated the native MS and middle-down results to have a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab.					
33118822	4	7	theme	C-terminal	940:949	arg1	lysine					951:956	C-terminal lysine clipping	940:965	C-terminal lysine clipping	940:965	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	5	8	theme	collision-induced	977:993	arg1	dissociation					995:1006	collision-induced dissociation	977:1006	collision-induced dissociation	977:1006	Based on collision-induced dissociation and ETD-combined middle-down analysis, sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits, respectively, were reached in a single LC run.					
33118822	1	9	theme	antibody	216:223	arg1	structures					231:240	therapeutic monoclonal antibody (mAb) structures	193:240	therapeutic monoclonal antibody (mAb) structures	193:240	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is critical for drug development but remains challenging due to the inherent structural heterogeneity.					
33118822	2	10	theme	intact	582:587	arg1	mass					589:592	intact mass and middle-down analyses	582:617	mass	589:592	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	2	11	theme	middle-down	598:608	arg1	analyses					610:617	intact mass and middle-down analyses	582:617	analyses	610:617	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	6	12	theme	Fc	1307:1308	arg1	fragment					1310:1317	Fc fragment	1307:1317	the Fc fragment Asn 61	1303:1324	The main glycan structure and relative abundance level were determined, and the glycosylation site was confirmed to be on the Fc fragment Asn 61.					
33118822	6	13	theme	glycosylation	1261:1273	arg1	site					1275:1278	the glycosylation site	1257:1278	the glycosylation site	1257:1278	The main glycan structure and relative abundance level were determined, and the glycosylation site was confirmed to be on the Fc fragment Asn 61.					
33118822	4	14	theme	denatured	838:846	arg1	analysis					860:867	native and denatured intact mass analysis	827:867	native and denatured intact mass analysis	827:867	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	7	15	theme	molecular	1425:1433	arg1	weight					1435:1440	molecular weight	1425:1440	molecular weight	1425:1440	We finally integrated the native MS and middle-down results to have a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab.					
33118822	7	16	theme	variants	1480:1487	arg1	detection					1412:1420	a more realistic detection	1395:1420	a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab	1395:1502	We finally integrated the native MS and middle-down results to have a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab.					
33118822	4	17	theme	clipping	958:965	arg1	lysine					951:956	C-terminal lysine clipping	940:965	C-terminal lysine clipping	940:965	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	4	18	theme	different	759:767	arg1	result					725:730	a result	723:730	a result	723:730	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	4	18	theme	different	759:767	arg1	proteoforms					769:779	more than 13 structurally different proteoforms	733:779	more than 13 structurally different proteoforms	733:779	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	8	19	theme	C-terminal	1635:1644	arg1	variants					1662:1669	unexpected C-terminal lysine-modified variants	1624:1669	unexpected C-terminal lysine-modified variants (dataset identifier PXD021287)	1624:1700	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	8	19	theme	C-terminal	1635:1644	arg1	PXD021287					1691:1699	dataset identifier PXD021287	1672:1699	dataset identifier PXD021287	1672:1699	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	1	20	theme	inherent	310:317	arg1	heterogeneity					330:342	the inherent structural heterogeneity	306:342	the inherent structural heterogeneity	306:342	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is critical for drug development but remains challenging due to the inherent structural heterogeneity.					
33118822	0	21	theme	Structural	133:142	arg1	Heterogeneity					144:156	Structural Heterogeneity	133:156	Structural Heterogeneity	133:156	Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural Heterogeneity.					
33118822	7	22	theme	realistic	1402:1410	arg1	detection					1412:1420	a more realistic detection	1395:1420	a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab	1395:1502	We finally integrated the native MS and middle-down results to have a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab.					
33118822	4	23	theme	mass	855:858	arg1	analysis					860:867	native and denatured intact mass analysis	827:867	native and denatured intact mass analysis	827:867	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	7	24	theme	native	1353:1358	arg1	MS					1360:1361	the native MS	1349:1361	the native MS	1349:1361	We finally integrated the native MS and middle-down results to have a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab.					
33118822	2	25	theme	minimal	519:525	arg1	artifacts					527:535	minimal artifacts	519:535	minimal artifacts	519:535	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	8	26	theme	comprehensive	1569:1581	arg1	characterization					1583:1598	the comprehensive characterization	1565:1598	the comprehensive characterization of adalimumab	1565:1612	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	0	27	dep	Trastuzumab	106:116	arg1	Heterogeneity					144:156	Structural Heterogeneity	133:156	Structural Heterogeneity	133:156	Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural Heterogeneity.					
33118822	8	28	theme	lysine-modified	1646:1660	arg1	variants					1662:1669	unexpected C-terminal lysine-modified variants	1624:1669	unexpected C-terminal lysine-modified variants (dataset identifier PXD021287)	1624:1700	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	8	28	theme	lysine-modified	1646:1660	arg1	PXD021287					1691:1699	dataset identifier PXD021287	1672:1699	dataset identifier PXD021287	1672:1699	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	0	29	theme	Intact	12:17	arg1	Analysis					24:31	Intact Mass Analysis	12:31	Intact Mass Analysis	12:31	Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural Heterogeneity.					
33118822	4	30	from	differences	907:917	arg1	glycosylation					922:934	glycosylation	922:934	glycosylation	922:934	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	4	30	from	differences	907:917	arg1	lysine					951:956	C-terminal lysine clipping	940:965	C-terminal lysine clipping	940:965	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	6	31	theme	structure	1197:1205	arg1	level					1230:1234	The main glycan structure and relative abundance level	1181:1234	The main glycan structure and relative abundance level	1181:1234	The main glycan structure and relative abundance level were determined, and the glycosylation site was confirmed to be on the Fc fragment Asn 61.					
33118822	6	32	gly	glycosylation	1261:1273	arg2	site					1275:1278	the glycosylation site	1257:1278	the glycosylation site	1257:1278	The main glycan structure and relative abundance level were determined, and the glycosylation site was confirmed to be on the Fc fragment Asn 61.					
33118822	1	33	theme	structural	319:328	arg1	heterogeneity					330:342	the inherent structural heterogeneity	306:342	the inherent structural heterogeneity	306:342	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is critical for drug development but remains challenging due to the inherent structural heterogeneity.					
33118822	8	34	theme	integrated	1518:1527	arg1	strategy					1529:1536	the integrated strategy	1514:1536	the integrated strategy	1514:1536	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	5	35	theme	sequence	1047:1054	arg1	coverage					1056:1063	sequence coverage	1047:1063	sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits	1047:1131	Based on collision-induced dissociation and ETD-combined middle-down analysis, sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits, respectively, were reached in a single LC run.					
33118822	2	36	theme	structural	430:439	arg1	heterogeneity					441:453	trastuzumab structural heterogeneity	418:453	trastuzumab structural heterogeneity	418:453	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	2	37	theme	sample	495:500	arg1	preparation					502:512	fast sample preparation	490:512	fast sample preparation with minimal artifacts	490:535	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	7	38	theme	weight	1435:1440	arg1	detection					1412:1420	a more realistic detection	1395:1420	a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab	1395:1502	We finally integrated the native MS and middle-down results to have a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab.					
33118822	7	39	theme	middle-down	1367:1377	arg1	results					1379:1385	middle-down results	1367:1385	middle-down results	1367:1385	We finally integrated the native MS and middle-down results to have a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab.					
33118822	4	40	theme	native	827:832	arg1	analysis					860:867	native and denatured intact mass analysis	827:867	native and denatured intact mass analysis	827:867	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	2	41	theme	fast	490:493	arg1	preparation					502:512	fast sample preparation	490:512	fast sample preparation with minimal artifacts	490:535	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	6	42	theme	relative	1211:1218	arg1	abundance					1220:1228	relative abundance	1211:1228	relative abundance	1211:1228	The main glycan structure and relative abundance level were determined, and the glycosylation site was confirmed to be on the Fc fragment Asn 61.					
33118822	6	43	theme	glycan	1190:1195	arg1	structure					1197:1205	main glycan structure	1185:1205	main glycan structure	1185:1205	The main glycan structure and relative abundance level were determined, and the glycosylation site was confirmed to be on the Fc fragment Asn 61.					
33118822	0	44	theme	Middle-Down	37:47	arg1	Spectrometry					54:65	Middle-Down Mass Spectrometry	37:65	Middle-Down Mass Spectrometry	37:65	Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural Heterogeneity.					
33118822	8	45	theme	adalimumab	1603:1612	arg1	characterization					1583:1598	the comprehensive characterization	1565:1598	the comprehensive characterization of adalimumab	1565:1612	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	9	46	theme	mAb	1776:1778	arg1	characterization					1805:1820	in-depth mAb structural heterogeneity characterization	1767:1820	in-depth mAb structural heterogeneity characterization	1767:1820	Overall, our integration strategy can be easily implemented for in-depth mAb structural heterogeneity characterization during pharmaceutical development and quality control.					
33118822	1	47	theme	structures	231:240	arg1	characterization					173:188	Comprehensive characterization	159:188	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures	159:240	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is critical for drug development but remains challenging due to the inherent structural heterogeneity.					
33118822	2	48	theme	prominent	466:474	arg1	advantages					476:485	prominent advantages	466:485	prominent advantages	466:485	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	8	49	theme	unexpected	1624:1633	arg1	variants					1662:1669	unexpected C-terminal lysine-modified variants	1624:1669	unexpected C-terminal lysine-modified variants (dataset identifier PXD021287)	1624:1700	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	8	49	theme	unexpected	1624:1633	arg1	PXD021287					1691:1699	dataset identifier PXD021287	1672:1699	dataset identifier PXD021287	1672:1699	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	9	50	theme	structural	1780:1789	arg1	characterization					1805:1820	in-depth mAb structural heterogeneity characterization	1767:1820	in-depth mAb structural heterogeneity characterization	1767:1820	Overall, our integration strategy can be easily implemented for in-depth mAb structural heterogeneity characterization during pharmaceutical development and quality control.					
33118822	3	51	theme	Q-TOF	702:706	arg1	instrument					708:717	an electron transfer dissociation (ETD)-enabled Q-TOF instrument	654:717	an electron transfer dissociation (ETD)-enabled Q-TOF instrument	654:717	Our methods were all developed on an electron transfer dissociation (ETD)-enabled Q-TOF instrument.					
33118822	5	52	theme	trastuzumab	1095:1105	arg1	Fc/2					1107:1110	trastuzumab Fc/2	1095:1110	trastuzumab Fc/2	1095:1110	Based on collision-induced dissociation and ETD-combined middle-down analysis, sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits, respectively, were reached in a single LC run.					
33118822	1	53	theme	drug	258:261	arg1	development					263:273	drug development	258:273	drug development	258:273	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is critical for drug development but remains challenging due to the inherent structural heterogeneity.					
33118822	9	54	theme	pharmaceutical	1829:1842	arg1	development					1844:1854	pharmaceutical development	1829:1854	pharmaceutical development	1829:1854	Overall, our integration strategy can be easily implemented for in-depth mAb structural heterogeneity characterization during pharmaceutical development and quality control.					
33118822	9	55	theme	heterogeneity	1791:1803	arg1	characterization					1805:1820	in-depth mAb structural heterogeneity characterization	1767:1820	in-depth mAb structural heterogeneity characterization	1767:1820	Overall, our integration strategy can be easily implemented for in-depth mAb structural heterogeneity characterization during pharmaceutical development and quality control.					
33118822	4	56	theme	collective	896:905	arg1	differences					907:917	the collective differences	892:917	the collective differences in glycosylation and C-terminal lysine clipping	892:965	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	0	57	theme	Mass	49:52	arg1	Spectrometry					54:65	Middle-Down Mass Spectrometry	37:65	Middle-Down Mass Spectrometry	37:65	Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural Heterogeneity.					
33118822	6	58	theme	abundance	1220:1228	arg1	level					1230:1234	The main glycan structure and relative abundance level	1181:1234	The main glycan structure and relative abundance level	1181:1234	The main glycan structure and relative abundance level were determined, and the glycosylation site was confirmed to be on the Fc fragment Asn 61.					
33118822	2	59	contain	has	462:464	arg2	advantages					476:485	prominent advantages	466:485	prominent advantages	466:485	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	2	59	contain	has	462:464	arg1	heterogeneity					441:453	trastuzumab structural heterogeneity	418:453	trastuzumab structural heterogeneity	418:453	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	2	59	contain	has	462:464	arg1	information					556:566	complementary information	542:566	complementary information obtained from intact mass and middle-down analyses	542:617	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	6	60	theme	main	1185:1188	arg1	structure					1197:1205	main glycan structure	1185:1205	main glycan structure	1185:1205	The main glycan structure and relative abundance level were determined, and the glycosylation site was confirmed to be on the Fc fragment Asn 61.					
33118822	5	61	theme	ETD-combined	1012:1023	arg1	analysis					1037:1044	ETD-combined middle-down analysis	1012:1044	ETD-combined middle-down analysis	1012:1044	Based on collision-induced dissociation and ETD-combined middle-down analysis, sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits, respectively, were reached in a single LC run.					
33118822	5	62	theme	%	1089:1089	arg1	values					1065:1070	sequence coverage values	1047:1070	sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits	1047:1131	Based on collision-induced dissociation and ETD-combined middle-down analysis, sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits, respectively, were reached in a single LC run.					
33118822	7	63	theme	sequence	1443:1450	arg1	variants					1452:1459	sequence variants	1443:1459	sequence variants	1443:1459	We finally integrated the native MS and middle-down results to have a more realistic detection of molecular weight, sequence variants, and glycosylation variants of trastuzumab.					
33118822	0	64	dep	Analysis	24:31	arg1	Approaches					67:76	Approaches	67:76	Approaches	67:76	Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural Heterogeneity.					
33118822	2	65	with	preparation	502:512	arg1	artifacts					527:535	minimal artifacts	519:535	minimal artifacts	519:535	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	1	66	theme	Comprehensive	159:171	arg1	characterization					173:188	Comprehensive characterization	159:188	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures	159:240	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is critical for drug development but remains challenging due to the inherent structural heterogeneity.					
33118822	5	67	theme	coverage	1056:1063	arg1	values					1065:1070	sequence coverage values	1047:1070	sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits	1047:1131	Based on collision-induced dissociation and ETD-combined middle-down analysis, sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits, respectively, were reached in a single LC run.					
33118822	4	68	theme	intact	848:853	arg1	analysis					860:867	native and denatured intact mass analysis	827:867	native and denatured intact mass analysis	827:867	As a result, more than 13 structurally different proteoforms were easily identified and quantified through native and denatured intact mass analysis, which may result from the collective differences in glycosylation and C-terminal lysine clipping.					
33118822	3	69	theme	-enabled	693:700	arg1	instrument					708:717	an electron transfer dissociation (ETD)-enabled Q-TOF instrument	654:717	an electron transfer dissociation (ETD)-enabled Q-TOF instrument	654:717	Our methods were all developed on an electron transfer dissociation (ETD)-enabled Q-TOF instrument.					
33118822	6	70	dep	fragment	1310:1317	arg1	Asn					1319:1321	Asn 61	1319:1324	the Fc fragment Asn 61	1303:1324	The main glycan structure and relative abundance level were determined, and the glycosylation site was confirmed to be on the Fc fragment Asn 61.					
33118822	2	71	theme	integrated	363:372	arg1	strategy					374:381	an integrated strategy	360:381	an integrated strategy	360:381	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	5	72	theme	middle-down	1025:1035	arg1	analysis					1037:1044	ETD-combined middle-down analysis	1012:1044	ETD-combined middle-down analysis	1012:1044	Based on collision-induced dissociation and ETD-combined middle-down analysis, sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits, respectively, were reached in a single LC run.					
33118822	9	73	theme	integration	1716:1726	arg1	strategy					1728:1735	our integration strategy	1712:1735	our integration strategy	1712:1735	Overall, our integration strategy can be easily implemented for in-depth mAb structural heterogeneity characterization during pharmaceutical development and quality control.					
33118822	5	74	theme	Fd	1121:1122	arg1	subunits					1124:1131	Fd subunits	1121:1131	Fd subunits	1121:1131	Based on collision-induced dissociation and ETD-combined middle-down analysis, sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits, respectively, were reached in a single LC run.					
33118822	2	75	theme	trastuzumab	418:428	arg1	heterogeneity					441:453	trastuzumab structural heterogeneity	418:453	trastuzumab structural heterogeneity	418:453	In this study, an integrated strategy has been developed to characterize trastuzumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.					
33118822	8	76	theme	identifier	1680:1689	arg1	variants					1662:1669	unexpected C-terminal lysine-modified variants	1624:1669	unexpected C-terminal lysine-modified variants (dataset identifier PXD021287)	1624:1700	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	8	76	theme	identifier	1680:1689	arg1	PXD021287					1691:1699	dataset identifier PXD021287	1672:1699	dataset identifier PXD021287	1672:1699	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	5	77	theme	single	1166:1171	arg1	run					1176:1178	a single LC run	1164:1178	a single LC run	1164:1178	Based on collision-induced dissociation and ETD-combined middle-down analysis, sequence coverage values of 28, 45, and 41% for trastuzumab Fc/2, Lc, and Fd subunits, respectively, were reached in a single LC run.					
33118822	0	78	theme	Mass	19:22	arg1	Analysis					24:31	Intact Mass Analysis	12:31	Intact Mass Analysis	12:31	Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural Heterogeneity.					
33118822	1	79	theme	therapeutic	193:203	arg1	mAb					226:228	mAb	226:228	mAb	226:228	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is critical for drug development but remains challenging due to the inherent structural heterogeneity.					
33118822	1	79	theme	therapeutic	193:203	arg1	antibody					216:223	therapeutic monoclonal antibody	193:223	therapeutic monoclonal antibody (mAb) structures	193:240	Comprehensive characterization of therapeutic monoclonal antibody (mAb) structures is critical for drug development but remains challenging due to the inherent structural heterogeneity.					
33118822	9	80	theme	in-depth	1767:1774	arg1	characterization					1805:1820	in-depth mAb structural heterogeneity characterization	1767:1820	in-depth mAb structural heterogeneity characterization	1767:1820	Overall, our integration strategy can be easily implemented for in-depth mAb structural heterogeneity characterization during pharmaceutical development and quality control.					
33118822	8	81	theme	dataset	1672:1678	arg1	variants					1662:1669	unexpected C-terminal lysine-modified variants	1624:1669	unexpected C-terminal lysine-modified variants (dataset identifier PXD021287)	1624:1700	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
33118822	8	81	theme	dataset	1672:1678	arg1	PXD021287					1691:1699	dataset identifier PXD021287	1672:1699	dataset identifier PXD021287	1672:1699	Applying the integrated strategy, we successfully completed the comprehensive characterization of adalimumab and found unexpected C-terminal lysine-modified variants (dataset identifier PXD021287).					
32830946	1	0	theme	distinct	172:179	arg1	signatures					181:190	distinct signatures	172:190	distinct signatures that are recognized by different glycan-binding proteins (GBPs) and pathogens	172:268	N-glycans on the cell surface provide distinct signatures that are recognized by different glycan-binding proteins (GBPs) and pathogens.					
32830946	0	1	theme	Enzymatic	109:117	arg1	Glycosylation					119:131	Selective Enzymatic Glycosylation	99:131	Selective Enzymatic Glycosylation	99:131	Synthesis of Asymmetric N-Glycans as Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation.					
32830946	5	2	theme	effect	1036:1041	arg1	GlcNAc					989:994	GlcNAc	989:994	GlcNAc	989:994	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	5	2	theme	effect	1036:1041	arg1	N-acetylglucosamine					968:986	the N-acetylglucosamine	964:986	the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding	964:1052	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	3	3	theme	core	553:556	arg1	substrates					558:567	designed common core substrates	537:567	designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation	537:640	In this work, we have developed an improved strategy for asymmetric N-glycan assembly and diversification using designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation.					
32830946	0	4	theme	Selective	99:107	arg1	Glycosylation					119:131	Selective Enzymatic Glycosylation	99:131	Selective Enzymatic Glycosylation	99:131	Synthesis of Asymmetric N-Glycans as Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation.					
32830946	4	5	theme	well-defined	660:671	arg1	application					784:794	a representative application	767:794	a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2)	767:889	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	5	theme	well-defined	660:671	arg1	glycans					673:679	The resulting 26 well-defined glycans	643:679	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae	643:736	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	6	used	used	743:746	arg2	application					784:794	a representative application	767:794	a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2)	767:889	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	6	used	used	743:746	arg2	glycans					673:679	The resulting 26 well-defined glycans	643:679	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae	643:736	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	5	7	theme	binding	910:916	arg1	affinity					918:925	distinct binding affinity	901:925	distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches	901:1101	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	4	8	from	residue	708:714	arg1	antennae					729:736	different antennae	719:736	different antennae	719:736	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	1	9	theme	cell	151:154	arg1	surface					156:162	the cell surface	147:162	the cell surface	147:162	N-glycans on the cell surface provide distinct signatures that are recognized by different glycan-binding proteins (GBPs) and pathogens.					
32830946	5	10	from	galactose	1071:1079	arg1	branches					1094:1101	different branches	1084:1101	different branches	1084:1101	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	4	11	from	virus	878:882	arg1	profile					799:805	profile	799:805	profile	799:805	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	12	theme	influenza	868:876	arg1	virus					878:882	the avian influenza virus	858:882	the avian influenza virus (H5N2)	858:889	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	12	theme	influenza	868:876	arg1	H5N2					885:888	H5N2	885:888	H5N2	885:888	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	3	13	theme	improved	460:467	arg1	strategy					469:476	an improved strategy	457:476	an improved strategy for asymmetric N-glycan assembly and diversification	457:529	In this work, we have developed an improved strategy for asymmetric N-glycan assembly and diversification using designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation.					
32830946	3	14	theme	designed	537:544	arg1	substrates					558:567	designed common core substrates	537:567	designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation	537:640	In this work, we have developed an improved strategy for asymmetric N-glycan assembly and diversification using designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation.					
32830946	2	15	theme	availability	399:410	arg1	lack					391:394	their lack	385:394	their lack of availability for studies	385:422	Most glycans in humans are asymmetric and isomeric, yet their biological functions are not well understood due to their lack of availability for studies.					
32830946	4	16	theme	avian	862:866	arg1	virus					878:882	the avian influenza virus	858:882	the avian influenza virus (H5N2)	858:889	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	16	theme	avian	862:866	arg1	H5N2					885:888	H5N2	885:888	H5N2	885:888	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	3	17	theme	common	546:551	arg1	substrates					558:567	designed common core substrates	537:567	designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation	537:640	In this work, we have developed an improved strategy for asymmetric N-glycan assembly and diversification using designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation.					
32830946	1	18	from	N-glycans	134:142	arg1	surface					156:162	the cell surface	147:162	the cell surface	147:162	N-glycans on the cell surface provide distinct signatures that are recognized by different glycan-binding proteins (GBPs) and pathogens.					
32830946	3	19	theme	selective	593:601	arg1	fucosylation					613:624	selective enzymatic fucosylation	593:624	selective enzymatic fucosylation	593:624	In this work, we have developed an improved strategy for asymmetric N-glycan assembly and diversification using designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation.					
32830946	4	20	theme	acid	703:706	arg1	residue					708:714	the sialic acid residue	692:714	the sialic acid residue on different antennae	692:736	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	0	21	theme	N-Glycans	24:32	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Asymmetric N-Glycans as Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation.	0:132	Synthesis of Asymmetric N-Glycans as Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation.					
32830946	6	22	theme	context-based	1162:1174	arg1	patterns					1188:1195	branch-biased and context-based recognition patterns	1144:1195	branch-biased and context-based recognition patterns	1144:1195	Overall, the microarray analysis showed branch-biased and context-based recognition patterns.					
32830946	1	23	theme	different	215:223	arg1	GBPs					250:253	GBPs	250:253	GBPs	250:253	N-glycans on the cell surface provide distinct signatures that are recognized by different glycan-binding proteins (GBPs) and pathogens.					
32830946	1	23	theme	different	215:223	arg1	proteins					240:247	different glycan-binding proteins	215:247	different glycan-binding proteins (GBPs)	215:254	N-glycans on the cell surface provide distinct signatures that are recognized by different glycan-binding proteins (GBPs) and pathogens.					
32830946	0	24	theme	Asymmetric	13:22	arg1	N-Glycans					24:32	Asymmetric N-Glycans	13:32	Asymmetric N-Glycans	13:32	Synthesis of Asymmetric N-Glycans as Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation.					
32830946	4	25	theme	binding	811:817	arg1	specificity					819:829	the binding specificity	807:829	the binding specificity of hemagglutinin (HA)	807:851	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	26	theme	representative	769:782	arg1	application					784:794	a representative application	767:794	a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2)	767:889	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	26	theme	representative	769:782	arg1	glycans					673:679	The resulting 26 well-defined glycans	643:679	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae	643:736	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	1	27	theme	glycan-binding	225:238	arg1	GBPs					250:253	GBPs	250:253	GBPs	250:253	N-glycans on the cell surface provide distinct signatures that are recognized by different glycan-binding proteins (GBPs) and pathogens.					
32830946	1	27	theme	glycan-binding	225:238	arg1	proteins					240:247	different glycan-binding proteins	215:247	different glycan-binding proteins (GBPs)	215:254	N-glycans on the cell surface provide distinct signatures that are recognized by different glycan-binding proteins (GBPs) and pathogens.					
32830946	0	28	theme	Common	37:42	arg1	Substrates					49:58	Common Core Substrates	37:58	Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation	37:131	Synthesis of Asymmetric N-Glycans as Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation.					
32830946	4	29	contain	carry	686:690	arg2	residue					708:714	the sialic acid residue	692:714	the sialic acid residue on different antennae	692:736	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	29	contain	carry	686:690	arg1	application					784:794	a representative application	767:794	a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2)	767:889	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	29	contain	carry	686:690	arg1	glycans					673:679	The resulting 26 well-defined glycans	643:679	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae	643:736	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	2	30	from	glycans	276:282	arg1	humans					287:292	humans	287:292	humans	287:292	Most glycans in humans are asymmetric and isomeric, yet their biological functions are not well understood due to their lack of availability for studies.					
32830946	4	31	theme	sialic	696:701	arg1	residue					708:714	the sialic acid residue	692:714	the sialic acid residue on different antennae	692:736	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	5	32	theme	different	1084:1092	arg1	branches					1094:1101	different branches	1084:1101	different branches	1084:1101	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	5	33	theme	minor	1030:1034	arg1	effect					1036:1041	only a minor effect	1023:1041	only a minor effect	1023:1041	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	5	34	attach	linked	954:959	arg2	epitope					946:952	the Neu5Ac-Gal epitope	931:952	the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding	931:1052	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	5	34	attach	linked	954:959	arg1	GlcNAc					989:994	GlcNAc	989:994	GlcNAc	989:994	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	5	34	attach	linked	954:959	arg1	N-acetylglucosamine					968:986	the N-acetylglucosamine	964:986	the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding	964:1052	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	3	35	theme	enzymatic	603:611	arg1	fucosylation					613:624	selective enzymatic fucosylation	593:624	selective enzymatic fucosylation	593:624	In this work, we have developed an improved strategy for asymmetric N-glycan assembly and diversification using designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation.					
32830946	3	36	theme	asymmetric	482:491	arg1	assembly					502:509	asymmetric N-glycan assembly	482:509	asymmetric N-glycan assembly	482:509	In this work, we have developed an improved strategy for asymmetric N-glycan assembly and diversification using designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation.					
32830946	0	37	theme	Core	44:47	arg1	Substrates					49:58	Common Core Substrates	37:58	Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation	37:131	Synthesis of Asymmetric N-Glycans as Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation.					
32830946	3	38	theme	N-glycan	493:500	arg1	assembly					502:509	asymmetric N-glycan assembly	482:509	asymmetric N-glycan assembly	482:509	In this work, we have developed an improved strategy for asymmetric N-glycan assembly and diversification using designed common core substrates prepared chemically for selective enzymatic fucosylation and sialylation.					
32830946	5	39	theme	different	1000:1008	arg1	branches					1010:1017	different branches	1000:1017	different branches	1000:1017	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	5	40	theme	Neu5Ac-Gal	935:944	arg1	epitope					946:952	the Neu5Ac-Gal epitope	931:952	the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding	931:1052	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	5	41	from	N-acetylglucosamine	968:986	arg1	binding					1046:1052	binding	1046:1052	binding	1046:1052	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	6	42	theme	microarray	1117:1126	arg1	analysis					1128:1135	the microarray analysis	1113:1135	the microarray analysis	1113:1135	Overall, the microarray analysis showed branch-biased and context-based recognition patterns.					
32830946	5	43	theme	branches	1010:1017	arg1	GlcNAc					989:994	GlcNAc	989:994	GlcNAc	989:994	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	5	43	theme	branches	1010:1017	arg1	N-acetylglucosamine					968:986	the N-acetylglucosamine	964:986	the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding	964:1052	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	2	44	theme	biological	333:342	arg1	functions					344:352	their biological functions	327:352	their biological functions	327:352	Most glycans in humans are asymmetric and isomeric, yet their biological functions are not well understood due to their lack of availability for studies.					
32830946	6	45	theme	branch-biased	1144:1156	arg1	patterns					1188:1195	branch-biased and context-based recognition patterns	1144:1195	branch-biased and context-based recognition patterns	1144:1195	Overall, the microarray analysis showed branch-biased and context-based recognition patterns.					
32830946	0	46	theme	Structural	64:73	arg1	Diversification					75:89	Structural Diversification	64:89	Structural Diversification through Selective Enzymatic Glycosylation	64:131	Synthesis of Asymmetric N-Glycans as Common Core Substrates for Structural Diversification through Selective Enzymatic Glycosylation.					
32830946	5	47	theme	terminal	1062:1069	arg1	galactose					1071:1079	the terminal galactose	1058:1079	the terminal galactose on different branches	1058:1101	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	2	48	theme	Most	271:274	arg1	glycans					276:282	Most glycans	271:282	Most glycans in humans	271:292	Most glycans in humans are asymmetric and isomeric, yet their biological functions are not well understood due to their lack of availability for studies.					
32830946	4	49	theme	different	719:727	arg1	antennae					729:736	different antennae	719:736	different antennae	719:736	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	6	50	theme	recognition	1176:1186	arg1	patterns					1188:1195	branch-biased and context-based recognition patterns	1144:1195	branch-biased and context-based recognition patterns	1144:1195	Overall, the microarray analysis showed branch-biased and context-based recognition patterns.					
32830946	4	51	theme	hemagglutinin	834:846	arg1	specificity					819:829	the binding specificity	807:829	the binding specificity of hemagglutinin (HA)	807:851	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	5	52	theme	distinct	901:908	arg1	affinity					918:925	distinct binding affinity	901:925	distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches	901:1101	We found distinct binding affinity for the Neu5Ac-Gal epitope linked to the N-acetylglucosamine (GlcNAc) of different branches and only a minor effect in binding for the terminal galactose on different branches.					
32830946	4	53	theme	resulting	647:655	arg1	application					784:794	a representative application	767:794	a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2)	767:889	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
32830946	4	53	theme	resulting	647:655	arg1	glycans					673:679	The resulting 26 well-defined glycans	643:679	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae	643:736	The resulting 26 well-defined glycans that carry the sialic acid residue on different antennae were used in a microarray as a representative application to profile the binding specificity of hemagglutinin (HA) from the avian influenza virus (H5N2).					
33380299	4	0	theme	structures	698:707	arg1	preservation					671:682	the preservation	667:682	the preservation of sialylated structures because of their important clinical - biological relevance	667:766	Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance.					
33380299	7	1	theme	profiling	1162:1170	arg1	translation					1120:1130	clinical translation	1111:1130	clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications	1111:1206	Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.					
33380299	2	2	from	cohorts	403:409	arg1	lack					364:367	the lack	360:367	the lack of verifications on large patient cohorts and suitable analytical technologies	360:446	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	2	3	theme	due	353:355	arg1	agencies					344:351	the regulatory agencies	329:351	the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies	329:446	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	7	4	from	applications	1195:1206	arg1	translation					1120:1130	clinical translation	1111:1130	clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications	1111:1206	Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.					
33380299	1	5	theme	high	163:166	arg1	importance					168:177	high importance	163:177	high importance	163:177	The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research.					
33380299	0	6	theme	Blood	94:98	arg1	Samples					100:106	Human Blood Samples	88:106	Human Blood Samples	88:106	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	0	7	from	Evaluation	0:9	arg1	Profile					77:83	the Global N-Glycosylation Profile	50:83	the Global N-Glycosylation Profile of Human Blood Samples	50:106	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	7	8	theme	diagnostic	1135:1144	arg1	profiling					1162:1170	diagnostic N-glycosylation profiling	1135:1170	diagnostic N-glycosylation profiling in molecular medicinal applications	1135:1206	Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.					
33380299	3	9	theme	handling	497:504	arg1	effect					468:473	the effect	464:473	the effect of human blood sample handling	464:504	In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection.					
33380299	0	10	theme	Human	88:92	arg1	Samples					100:106	Human Blood Samples	88:106	Human Blood Samples	88:106	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	4	11	theme	Special	637:643	arg1	attention					645:653	Special attention	637:653	Special attention	637:653	Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance.					
33380299	7	12	theme	N-glycosylation	1146:1160	arg1	profiling					1162:1170	diagnostic N-glycosylation profiling	1135:1170	diagnostic N-glycosylation profiling in molecular medicinal applications	1135:1206	Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.					
33380299	3	13	theme	N-glycosylation	527:541	arg1	profiling					543:551	N-glycosylation profiling	527:551	N-glycosylation profiling	527:551	In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection.					
33380299	4	14	theme	important	726:734	arg1	relevance					758:766	their important clinical - biological relevance	720:766	their important clinical - biological relevance	720:766	Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance.					
33380299	5	15	theme	blood	856:860	arg1	samples					862:868	the collected total blood samples	836:868	the collected total blood samples prior to analysis to obtain unbiased results	836:913	Our results suggested that it is adequate to refrigerate and store the collected total blood samples prior to analysis to obtain unbiased results.					
33380299	4	16	theme	relevance	758:766	arg1	structures					698:707	sialylated structures	687:707	sialylated structures because of their important clinical - biological relevance	687:766	Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance.					
33380299	2	17	theme	glycan	268:273	arg1	biomarker					275:283	no glycan biomarker	265:283	no glycan biomarker	265:283	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	0	18	theme	Samples	100:106	arg1	Profile					77:83	the Global N-Glycosylation Profile	50:83	the Global N-Glycosylation Profile of Human Blood Samples	50:106	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	0	19	from	Effects	39:45	arg1	Profile					77:83	the Global N-Glycosylation Profile	50:83	the Global N-Glycosylation Profile of Human Blood Samples	50:106	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	1	20	theme	clinical	217:224	arg1	research					226:233	applied clinical research	209:233	applied clinical research	209:233	The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research.					
33380299	2	21	theme	verifications	372:384	arg1	lack					364:367	the lack	360:367	the lack of verifications on large patient cohorts and suitable analytical technologies	360:446	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	2	22	theme	clinical	307:314	arg1	diagnosis					316:324	clinical diagnosis	307:324	clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies	307:446	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	7	23	theme	clinical	1111:1118	arg1	translation					1120:1130	clinical translation	1111:1130	clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications	1111:1206	Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.					
33380299	7	24	from	profiling	1162:1170	arg1	applications					1195:1206	molecular medicinal applications	1175:1206	molecular medicinal applications	1175:1206	Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.					
33380299	0	25	theme	Processing	23:32	arg1	Effects					39:45	Possible Processing Time Effects	14:45	Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples	14:106	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	6	26	theme	handling	976:983	arg1	practice					951:958	the good practice	942:958	the good practice of serum sample handling in order to prevent decomposition of the sialylated structures	942:1046	Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures.					
33380299	3	27	theme	capillary	556:564	arg1	electrophoresis					566:580	capillary electrophoresis	556:580	capillary electrophoresis	556:580	In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection.					
33380299	4	28	dep	important	726:734	arg1	biological					747:756	biological	747:756	biological	747:756	Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance.					
33380299	4	28	dep	important	726:734	arg1	clinical					736:743	clinical	736:743	clinical	736:743	Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance.					
33380299	5	29	theme	total	850:854	arg1	samples					862:868	the collected total blood samples	836:868	the collected total blood samples prior to analysis to obtain unbiased results	836:913	Our results suggested that it is adequate to refrigerate and store the collected total blood samples prior to analysis to obtain unbiased results.					
33380299	0	30	theme	Possible	14:21	arg1	Effects					39:45	Possible Processing Time Effects	14:45	Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples	14:106	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	6	31	theme	sample	969:974	arg1	handling					976:983	serum sample handling	963:983	serum sample handling in order to prevent decomposition of the sialylated structures	963:1046	Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures.					
33380299	3	32	theme	sample	490:495	arg1	handling					497:504	human blood sample handling	478:504	human blood sample handling	478:504	In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection.					
33380299	5	33	theme	unbiased	898:905	arg1	results					907:913	unbiased results	898:913	unbiased results	898:913	Our results suggested that it is adequate to refrigerate and store the collected total blood samples prior to analysis to obtain unbiased results.					
33380299	1	34	theme	applied	209:215	arg1	research					226:233	applied clinical research	209:233	applied clinical research	209:233	The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research.					
33380299	0	35	theme	Effects	39:45	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.	0:107	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	6	36	theme	serum	963:967	arg1	handling					976:983	serum sample handling	963:983	serum sample handling in order to prevent decomposition of the sialylated structures	963:1046	Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures.					
33380299	0	37	from	Profile	77:83	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.	0:107	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	2	38	theme	patient	395:401	arg1	cohorts					403:409	large patient cohorts	389:409	large patient cohorts	389:409	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	0	39	theme	Time	34:37	arg1	Effects					39:45	Possible Processing Time Effects	14:45	Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples	14:106	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	7	40	theme	procedure	1074:1082	arg1	standardization					1084:1098	procedure standardization	1074:1098	procedure standardization	1074:1098	Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.					
33380299	2	41	theme	analytical	424:433	arg1	technologies					435:446	suitable analytical technologies	415:446	suitable analytical technologies	415:446	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	2	42	theme	large	389:393	arg1	cohorts					403:409	large patient cohorts	389:409	large patient cohorts	389:409	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	7	43	theme	molecular	1175:1183	arg1	applications					1195:1206	molecular medicinal applications	1175:1206	molecular medicinal applications	1175:1206	Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.					
33380299	3	44	theme	blood	484:488	arg1	handling					497:504	human blood sample handling	478:504	human blood sample handling	478:504	In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection.					
33380299	4	45	gly	sialylated	687:696	arg1	structures					698:707	sialylated structures	687:707	sialylated structures because of their important clinical - biological relevance	687:766	Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance.					
33380299	2	46	from	lack	364:367	arg1	technologies					435:446	suitable analytical technologies	415:446	suitable analytical technologies	415:446	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	2	46	from	lack	364:367	arg1	cohorts					403:409	large patient cohorts	389:409	large patient cohorts	389:409	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	2	47	theme	regulatory	333:342	arg1	agencies					344:351	the regulatory agencies	329:351	the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies	329:446	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	2	48	from	technologies	435:446	arg1	lack					364:367	the lack	360:367	the lack of verifications on large patient cohorts and suitable analytical technologies	360:446	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	6	49	theme	good	946:949	arg1	practice					951:958	the good practice	942:958	the good practice of serum sample handling in order to prevent decomposition of the sialylated structures	942:1046	Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures.					
33380299	3	50	theme	human	478:482	arg1	handling					497:504	human blood sample handling	478:504	human blood sample handling	478:504	In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection.					
33380299	3	51	theme	fluorescence	613:624	arg1	detection					626:634	high sensitivity fluorescence detection	596:634	high sensitivity fluorescence detection	596:634	In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection.					
33380299	7	52	theme	medicinal	1185:1193	arg1	applications					1195:1206	molecular medicinal applications	1175:1206	molecular medicinal applications	1175:1206	Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.					
33380299	3	53	theme	high	596:599	arg1	sensitivity					601:611	high sensitivity	596:611	high sensitivity fluorescence detection	596:634	In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection.					
33380299	2	54	from	verifications	372:384	arg1	technologies					435:446	suitable analytical technologies	415:446	suitable analytical technologies	415:446	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	2	54	from	verifications	372:384	arg1	cohorts					403:409	large patient cohorts	389:409	large patient cohorts	389:409	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	6	55	theme	structures	1037:1046	arg1	decomposition					1005:1017	decomposition	1005:1017	decomposition of the sialylated structures	1005:1046	Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures.					
33380299	5	56	theme	collected	840:848	arg1	samples					862:868	the collected total blood samples	836:868	the collected total blood samples prior to analysis to obtain unbiased results	836:913	Our results suggested that it is adequate to refrigerate and store the collected total blood samples prior to analysis to obtain unbiased results.					
33380299	6	57	gly	sialylated	1026:1035	arg1	structures					1037:1046	the sialylated structures	1022:1046	the sialylated structures	1022:1046	Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures.					
33380299	1	58	theme	N-glycan	128:135	arg1	profiling					137:145	N-glycan profiling	128:145	N-glycan profiling	128:145	The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research.					
33380299	6	59	theme	sialylated	1026:1035	arg1	structures					1037:1046	the sialylated structures	1022:1046	the sialylated structures	1022:1046	Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures.					
33380299	0	60	theme	N-Glycosylation	61:75	arg1	Profile					77:83	the Global N-Glycosylation Profile	50:83	the Global N-Glycosylation Profile of Human Blood Samples	50:106	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	5	61	theme	prior	870:874	arg1	samples					862:868	the collected total blood samples	836:868	the collected total blood samples prior to analysis to obtain unbiased results	836:913	Our results suggested that it is adequate to refrigerate and store the collected total blood samples prior to analysis to obtain unbiased results.					
33380299	3	62	theme	sensitivity	601:611	arg1	detection					626:634	high sensitivity fluorescence detection	596:634	high sensitivity fluorescence detection	596:634	In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection.					
33380299	1	63	theme	profiling	137:145	arg1	utilization					113:123	The utilization	109:123	The utilization of N-glycan profiling	109:145	The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research.					
33380299	0	64	theme	Global	54:59	arg1	Profile					77:83	the Global N-Glycosylation Profile	50:83	the Global N-Glycosylation Profile of Human Blood Samples	50:106	Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples.					
33380299	2	65	theme	suitable	415:422	arg1	technologies					435:446	suitable analytical technologies	415:446	suitable analytical technologies	415:446	However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies.					
33380299	4	66	theme	sialylated	687:696	arg1	structures					698:707	sialylated structures	687:707	sialylated structures because of their important clinical - biological relevance	687:766	Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance.					
33380299	7	67	from	translation	1120:1130	arg1	applications					1195:1206	molecular medicinal applications	1175:1206	molecular medicinal applications	1175:1206	Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.					
32162920	5	0	theme	least-squares	757:769	arg1	PLS-DA					794:799	PLS-DA	794:799	PLS-DA	794:799	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	5	0	theme	least-squares	757:769	arg1	analysis					784:791	Partial least-squares discriminant analysis	749:791	Partial least-squares discriminant analysis (PLS-DA)	749:800	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	6	1	theme	glycan	1012:1017	arg1	isomers					1019:1025	these glycan isomers	1006:1025	these glycan isomers	1006:1025	Moreover, some of these glycan isomers also seemed to be related with the progression of CIA, especially H6N5S2 and H6N5S3_2, as their overexpression increased with the clinical score of the pathology.					
32162920	1	2	theme	immunoaffinity	152:165	arg1	IAC					183:185	IAC	183:185	IAC	183:185	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	1	2	theme	immunoaffinity	152:165	arg1	chromatography					167:180	immunoaffinity chromatography	152:180	immunoaffinity chromatography (IAC)	152:186	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	5	3	theme	discriminant	771:782	arg1	PLS-DA					794:799	PLS-DA	794:799	PLS-DA	794:799	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	5	3	theme	discriminant	771:782	arg1	analysis					784:791	Partial least-squares discriminant analysis	749:791	Partial least-squares discriminant analysis (PLS-DA)	749:800	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	7	4	theme	diseases	1411:1418	arg1	changes					1376:1382	glycosylation changes	1362:1382	glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered	1362:1473	Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.					
32162920	2	5	used	used	348:351	arg2	SDS-PAGE					317:324	SDS-PAGE	317:324	SDS-PAGE	317:324	SDS-PAGE and nanoLC-MS/MS were used to monitor the IAC purification performance.					
32162920	2	5	used	used	348:351	arg2	nanoLC-MS/MS					330:341	nanoLC-MS/MS	330:341	nanoLC-MS/MS	330:341	SDS-PAGE and nanoLC-MS/MS were used to monitor the IAC purification performance.					
32162920	4	6	theme	glycan	669:674	arg1	isomers					676:682	the most meaningful glycan isomers	649:682	the most meaningful glycan isomers for the discrimination between control and pathological samples	649:746	In addition, multivariate data analysis was applied to identify the most meaningful glycan isomers for the discrimination between control and pathological samples.					
32162920	0	7	from	Alterations	0:10	arg1	Profile					26:32	the Glycan Profile	15:32	the Glycan Profile of Mouse Transferrin	15:53	Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.					
32162920	5	8	dep	revealed	802:809	arg1	corresponding					906:918	corresponding	906:918	corresponding to highly sialylated structures	906:950	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	7	9	theme	glycosylation	1362:1374	arg1	changes					1376:1382	glycosylation changes	1362:1382	glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered	1362:1473	Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.					
32162920	4	10	theme	pathological	727:738	arg1	samples					740:746	pathological samples	727:746	pathological samples	727:746	In addition, multivariate data analysis was applied to identify the most meaningful glycan isomers for the discrimination between control and pathological samples.					
32162920	0	11	theme	Collagen-Induced	72:87	arg1	Arthritis					89:97	Collagen-Induced Arthritis	72:97	Collagen-Induced Arthritis	72:97	Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.					
32162920	7	12	theme	other	1392:1396	arg1	diseases					1411:1418	many other inflammatory diseases	1387:1418	many other inflammatory diseases	1387:1418	Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.					
32162920	5	13	dep	corresponding	906:918	arg1	H5N4S3_2					977:984	H5N4S3_2	977:984	H5N4S3_2	977:984	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	5	13	dep	corresponding	906:918	arg1	H6N5S3_2					953:960	H6N5S3_2	953:960	H6N5S3_2	953:960	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	5	13	dep	corresponding	906:918	arg1	H6N5S3_3					963:970	H6N5S3_3	963:970	H6N5S3_3	963:970	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	1	14	from	samples	141:147	arg1	purification					112:123	Transferrin purification	100:123	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC)	100:186	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	3	15	theme	/[13C6	489:494	arg1	-aniline					496:503	[12C6]/[13C6]-aniline	483:503	[12C6]/[13C6]-aniline	483:503	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	5	16	theme	isomers	847:853	arg1	five					816:819	five	816:819	five	816:819	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	5	16	theme	isomers	847:853	arg1	isomers					847:853	fifteen mTf glycan isomers	828:853	fifteen mTf glycan isomers	828:853	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	6	17	with	related	1045:1051	arg1	progression					1062:1072	the progression	1058:1072	the progression of CIA, especially H6N5S2 and H6N5S3_2	1058:1111	Moreover, some of these glycan isomers also seemed to be related with the progression of CIA, especially H6N5S2 and H6N5S3_2, as their overexpression increased with the clinical score of the pathology.					
32162920	4	18	theme	multivariate	598:609	arg1	analysis					616:623	multivariate data analysis	598:623	multivariate data analysis	598:623	In addition, multivariate data analysis was applied to identify the most meaningful glycan isomers for the discrimination between control and pathological samples.					
32162920	7	19	theme	glycan-based	1272:1283	arg1	biomarkers					1285:1294	glycan-based biomarkers	1272:1294	glycan-based biomarkers of CIA	1272:1301	Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.					
32162920	3	20	theme	mouse	438:442	arg1	mTf					457:459	mTf	457:459	mTf	457:459	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	3	20	theme	mouse	438:442	arg1	transferrin					444:454	mouse transferrin	438:454	mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline	438:503	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	5	21	gly	sialylated	930:939	arg1	structures					941:950	highly sialylated structures	923:950	highly sialylated structures	923:950	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	6	22	theme	CIA	1077:1079	arg1	progression					1062:1072	the progression	1058:1072	the progression of CIA, especially H6N5S2 and H6N5S3_2	1058:1111	Moreover, some of these glycan isomers also seemed to be related with the progression of CIA, especially H6N5S2 and H6N5S3_2, as their overexpression increased with the clinical score of the pathology.					
32162920	5	23	theme	CIA	888:890	arg1	biomarkers					874:883	potential biomarkers	864:883	potential biomarkers of CIA	864:890	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	3	24	used	used	509:512	arg2	quantification					420:433	a relative quantification	409:433	a relative quantification	409:433	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	3	24	used	used	509:512	arg2	Afterward					398:406	Afterward	398:406	Afterward	398:406	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	7	25	theme	many	1387:1390	arg1	diseases					1411:1418	many other inflammatory diseases	1387:1418	many other inflammatory diseases	1387:1418	Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.					
32162920	7	26	theme	valuable	1243:1250	arg1	information					1252:1262	valuable information	1243:1262	valuable information	1243:1262	Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.					
32162920	7	27	theme	inflammatory	1398:1409	arg1	diseases					1411:1418	many other inflammatory diseases	1387:1418	many other inflammatory diseases	1387:1418	Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.					
32162920	2	28	theme	purification	372:383	arg1	performance					385:395	the IAC purification performance	364:395	the IAC purification performance	364:395	SDS-PAGE and nanoLC-MS/MS were used to monitor the IAC purification performance.					
32162920	3	29	theme	glycan	557:562	arg1	profile					564:570	the glycan profile	553:570	the glycan profile of CIA mice	553:582	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	2	30	theme	IAC	368:370	arg1	performance					385:395	the IAC purification performance	364:395	the IAC purification performance	364:395	SDS-PAGE and nanoLC-MS/MS were used to monitor the IAC purification performance.					
32162920	0	31	theme	Glycan	19:24	arg1	Profile					26:32	the Glycan Profile	15:32	the Glycan Profile of Mouse Transferrin	15:53	Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.					
32162920	1	32	theme	collagen-induced	275:290	arg1	arthritis					292:300	collagen-induced arthritis	275:300	collagen-induced arthritis (CIA) samples	275:314	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	1	32	theme	collagen-induced	275:290	arg1	CIA					303:305	CIA	303:305	CIA	303:305	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	5	33	theme	sialylated	930:939	arg1	structures					941:950	highly sialylated structures	923:950	highly sialylated structures	923:950	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	4	34	theme	data	611:614	arg1	analysis					616:623	multivariate data analysis	598:623	multivariate data analysis	598:623	In addition, multivariate data analysis was applied to identify the most meaningful glycan isomers for the discrimination between control and pathological samples.					
32162920	1	35	theme	arthritis	292:300	arg1	samples					308:314	collagen-induced arthritis (CIA) samples	275:314	collagen-induced arthritis (CIA) samples	275:314	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	1	36	from	glycans	264:270	arg1	samples					308:314	collagen-induced arthritis (CIA) samples	275:314	collagen-induced arthritis (CIA) samples	275:314	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	1	37	theme	Transferrin	100:110	arg1	purification					112:123	Transferrin purification	100:123	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC)	100:186	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	3	38	theme	glycan	462:467	arg1	isomers					469:475	mouse transferrin (mTf) glycan isomers	438:475	mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline	438:503	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	0	39	from	Insights	60:67	arg1	Arthritis					89:97	Collagen-Induced Arthritis	72:97	Collagen-Induced Arthritis	72:97	Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.					
32162920	3	40	theme	CIA	575:577	arg1	mice					579:582	CIA mice	575:582	CIA mice	575:582	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	5	41	theme	potential	864:872	arg1	biomarkers					874:883	potential biomarkers	864:883	potential biomarkers of CIA	864:890	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	5	42	theme	mTf	836:838	arg1	isomers					847:853	fifteen mTf glycan isomers	828:853	fifteen mTf glycan isomers	828:853	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	3	43	theme	relative	411:418	arg1	quantification					420:433	a relative quantification	409:433	a relative quantification	409:433	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	3	43	theme	relative	411:418	arg1	Afterward					398:406	Afterward	398:406	Afterward	398:406	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	3	44	theme	mice	579:582	arg1	profile					564:570	the glycan profile	553:570	the glycan profile of CIA mice	553:582	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	5	45	theme	glycan	840:845	arg1	isomers					847:853	fifteen mTf glycan isomers	828:853	fifteen mTf glycan isomers	828:853	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	0	46	theme	Transferrin	43:53	arg1	Profile					26:32	the Glycan Profile	15:32	the Glycan Profile of Mouse Transferrin	15:53	Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.					
32162920	5	47	dep	isomers	847:853	arg1	out					821:823	out	821:823	out	821:823	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	0	48	theme	Mouse	37:41	arg1	Transferrin					43:53	Mouse Transferrin	37:53	Mouse Transferrin	37:53	Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.					
32162920	7	49	theme	door	1324:1327	arg1	open					1329:1332	the door open	1320:1332	the door open	1320:1332	Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.					
32162920	7	50	theme	CIA	1299:1301	arg1	biomarkers					1285:1294	glycan-based biomarkers	1272:1294	glycan-based biomarkers of CIA	1272:1301	Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.					
32162920	3	51	from	alterations	538:548	arg1	profile					564:570	the glycan profile	553:570	the glycan profile of CIA mice	553:582	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	6	52	theme	pathology	1179:1187	arg1	score					1166:1170	the clinical score	1153:1170	the clinical score of the pathology	1153:1187	Moreover, some of these glycan isomers also seemed to be related with the progression of CIA, especially H6N5S2 and H6N5S3_2, as their overexpression increased with the clinical score of the pathology.					
32162920	1	53	theme	mice	130:133	arg1	samples					141:147	mice serum samples	130:147	mice serum samples	130:147	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	6	54	theme	clinical	1157:1164	arg1	score					1166:1170	the clinical score	1153:1170	the clinical score of the pathology	1153:1187	Moreover, some of these glycan isomers also seemed to be related with the progression of CIA, especially H6N5S2 and H6N5S3_2, as their overexpression increased with the clinical score of the pathology.					
32162920	1	55	theme	serum	135:139	arg1	samples					141:147	mice serum samples	130:147	mice serum samples	130:147	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	3	56	theme	isomers	469:475	arg1	quantification					420:433	a relative quantification	409:433	a relative quantification	409:433	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	3	56	theme	isomers	469:475	arg1	Afterward					398:406	Afterward	398:406	Afterward	398:406	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	0	57	theme	New	56:58	arg1	Insights					60:67	New Insights	56:67	Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.	0:98	Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.					
32162920	4	58	theme	meaningful	658:667	arg1	isomers					676:682	the most meaningful glycan isomers	649:682	the most meaningful glycan isomers for the discrimination between control and pathological samples	649:746	In addition, multivariate data analysis was applied to identify the most meaningful glycan isomers for the discrimination between control and pathological samples.					
32162920	3	59	theme	transferrin	444:454	arg1	isomers					469:475	mouse transferrin (mTf) glycan isomers	438:475	mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline	438:503	Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice.					
32162920	1	60	theme	possible	224:231	arg1	modifications					233:245	the possible modifications	220:245	the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples	220:314	Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples.					
32162920	5	61	theme	Partial	749:755	arg1	PLS-DA					794:799	PLS-DA	794:799	PLS-DA	794:799	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	5	61	theme	Partial	749:755	arg1	analysis					784:791	Partial least-squares discriminant analysis	749:791	Partial least-squares discriminant analysis (PLS-DA)	749:800	Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2).					
32162920	0	62	dep	Alterations	0:10	arg1	Insights					60:67	New Insights	56:67	Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.	0:98	Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.					
32162920	7	63	theme	established	1201:1211	arg1	methodology					1213:1223	the established methodology	1197:1223	the established methodology	1197:1223	Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.					
32865981	9	0	theme	ovalbumin	1985:1993	arg1	N-glycans					1995:2003	ovalbumin N-glycans	1985:2003	ovalbumin N-glycans from different sources	1985:2026	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	9	1	theme	isomeric	2206:2213	arg1	structure					2215:2223	each isomeric structure	2201:2223	each isomeric structure	2201:2223	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	6	2	theme	additional	1305:1314	arg1	information					1327:1337	additional structural information	1305:1337	additional structural information that can differentiate isomers from conformers	1305:1384	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	7	3	theme	isomer-specific	1535:1549	arg1	fragments					1551:1559	isomer-specific fragments	1535:1559	isomer-specific fragments	1535:1559	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	6	4	theme	IMS	1210:1212	arg1	devices					1214:1220	IMS devices	1210:1220	IMS devices	1210:1220	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	8	5	theme	N-linked	1791:1798	arg1	glycans					1800:1806	N-linked glycans	1791:1806	N-linked glycans released from ovalbumin	1791:1830	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	7	6	theme	mixtures	1469:1476	arg1	analysis					1441:1448	Online electronic excitation dissociation (EED) MS/MS analysis	1387:1448	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation	1387:1504	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	3	7	theme	permethylated	731:743	arg1	derivatives					745:755	their permethylated derivatives	725:755	their permethylated derivatives	725:755	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	6	8	theme	isomers	1282:1288	arg1	separation					1268:1277	better separation	1261:1277	better separation of isomers	1261:1288	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	9	9	gly	glycosylation	2128:2140	arg2	level					2165:2169	the isomer level	2154:2169	the isomer level	2154:2169	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	1	10	theme	powerful	194:201	arg1	tool					203:206	a powerful tool	192:206	a powerful tool for glycan structural characterization due to its ability to separate isomers	192:284	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	4	11	theme	reducing-end	875:886	arg1	reduction					888:896	reducing-end reduction	875:896	reducing-end reduction	875:896	Notably, reducing-end reduction, commonly performed to remove anomerism-induced chromatographic peak splitting, did not eliminate the conformational heterogeneity of permethylated glycans in the gas phase.					
32865981	7	12	theme	isomeric	1453:1460	arg1	mixtures					1469:1476	isomeric glycan mixtures	1453:1476	isomeric glycan mixtures following G-TIMS separation	1453:1504	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	3	13	from	derivatives	745:755	arg1	forms					789:793	both nonreduced and reduced forms	761:793	both nonreduced and reduced forms	761:793	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	7	14	theme	EED	1430:1432	arg1	analysis					1441:1448	Online electronic excitation dissociation (EED) MS/MS analysis	1387:1448	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation	1387:1504	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	9	15	theme	G-TIMS	2044:2049	arg1	approach					2061:2068	the G-TIMS EED MS/MS approach	2040:2068	the G-TIMS EED MS/MS approach	2040:2068	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	1	16	theme	structural	219:228	arg1	characterization					230:245	glycan structural characterization	212:245	glycan structural characterization due to its ability	212:264	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	6	17	theme	better	1261:1266	arg1	separation					1268:1277	better separation	1261:1277	better separation of isomers	1261:1288	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	9	18	theme	MS/MS	2055:2059	arg1	approach					2061:2068	the G-TIMS EED MS/MS approach	2040:2068	the G-TIMS EED MS/MS approach	2040:2068	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	3	19	theme	ion	654:656	arg1	behavior					667:674	the ion mobility behavior	650:674	the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms,	650:794	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	8	20	theme	mobility	1854:1861	arg1	features					1863:1870	several mobility features	1846:1870	several mobility features previously thought to arise from isomeric structures	1846:1923	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	5	21	theme	resolving	1086:1094	arg1	power					1096:1100	a mobility resolving power	1075:1100	a mobility resolving power of ∼100	1075:1108	At a mobility resolving power of ∼100, 14 out of 22 structures showed more than one conformation.					
32865981	1	22	theme	mobility-mass	146:158	arg1	spectrometry					160:171	Ion mobility-mass spectrometry	142:171	Ion mobility-mass spectrometry (IM-MS)	142:179	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	1	22	theme	mobility-mass	146:158	arg1	IM-MS					174:178	IM-MS	174:178	IM-MS	174:178	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	4	23	theme	chromatographic	946:960	arg1	peak					962:965	anomerism-induced chromatographic peak	928:965	anomerism-induced chromatographic peak splitting	928:975	Notably, reducing-end reduction, commonly performed to remove anomerism-induced chromatographic peak splitting, did not eliminate the conformational heterogeneity of permethylated glycans in the gas phase.					
32865981	6	24	theme	mobility	1232:1239	arg1	power					1251:1255	high mobility resolving power	1227:1255	high mobility resolving power for better separation of isomers	1227:1288	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	7	25	theme	excitation	1405:1414	arg1	analysis					1441:1448	Online electronic excitation dissociation (EED) MS/MS analysis	1387:1448	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation	1387:1504	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	3	26	theme	ion	830:832	arg1	G-TIMS					857:862	G-TIMS	857:862	G-TIMS	857:862	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	3	26	theme	ion	830:832	arg1	spectrometry					843:854	gated-trapped ion mobility spectrometry	816:854	gated-trapped ion mobility spectrometry (G-TIMS)	816:863	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	7	27	theme	G-TIMS	1488:1493	arg1	separation					1495:1504	G-TIMS separation	1488:1504	G-TIMS separation	1488:1504	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	7	28	theme	Online	1387:1392	arg1	analysis					1441:1448	Online electronic excitation dissociation (EED) MS/MS analysis	1387:1448	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation	1387:1504	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	9	29	theme	batch-to-batch	2099:2112	arg1	variations					2114:2123	the batch-to-batch variations	2095:2123	the batch-to-batch variations in glycosylation profiles at the isomer level	2095:2169	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	0	30	theme	Mobility	59:66	arg1	Spectrometry					128:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	7	31	with	isomers	1672:1678	arg1	evidence					1693:1700	minimal evidence	1685:1700	minimal evidence of any ambiguity resulting from the presence of conformers	1685:1759	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	4	32	theme	glycans	1046:1052	arg1	heterogeneity					1015:1027	the conformational heterogeneity	996:1027	the conformational heterogeneity of permethylated glycans in the gas phase	996:1069	Notably, reducing-end reduction, commonly performed to remove anomerism-induced chromatographic peak splitting, did not eliminate the conformational heterogeneity of permethylated glycans in the gas phase.					
32865981	0	33	theme	Excitation	92:101	arg1	Spectrometry					128:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	7	34	theme	confident	1644:1652	arg1	identification					1654:1667	confident identification	1644:1667	confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers	1644:1759	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	7	35	theme	dissociation	1416:1427	arg1	analysis					1441:1448	Online electronic excitation dissociation (EED) MS/MS analysis	1387:1448	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation	1387:1504	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	0	36	theme	Tandem	116:121	arg1	Spectrometry					128:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	2	37	theme	isomer	539:544	arg1	separation					546:555	isomer separation	539:555	isomer separation	539:555	However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers.					
32865981	9	38	theme	structure	2215:2223	arg1	assignment					2187:2196	confident assignment	2177:2196	confident assignment of each isomeric structure	2177:2223	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	0	39	theme	Accurate	0:7	arg1	Identification					9:22	Accurate Identification	0:22	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	0:139	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	1	40	theme	collision	298:306	arg1	section					314:320	collision cross section	298:320	collision cross section (CCS) values that facilitate structural assignment	298:371	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	1	40	theme	collision	298:306	arg1	CCS					323:325	CCS	323:325	CCS	323:325	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	8	41	theme	structure	1953:1961	arg1	conformers					1930:1939	conformers	1930:1939	conformers of a single structure	1930:1961	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	7	42	theme	conformers	1750:1759	arg1	presence					1738:1745	the presence	1734:1745	the presence of conformers	1734:1759	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	2	43	theme	gas-phase	455:463	arg1	conformations					465:477	multiple gas-phase conformations	446:477	multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers	446:641	However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers.					
32865981	1	44	theme	section	314:320	arg1	values					328:333	collision cross section (CCS) values	298:333	collision cross section (CCS) values that facilitate structural assignment	298:371	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	3	45	theme	several	679:685	arg1	sets					687:690	several sets	679:690	several sets	679:690	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	3	45	theme	several	679:685	arg1	glycans					704:710	isomeric glycans	695:710	isomeric glycans	695:710	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	0	46	theme	Glycans	36:42	arg1	Identification					9:22	Accurate Identification	0:22	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	0:139	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	7	47	theme	mass	1601:1604	arg1	spectra					1606:1612	nearly identical tandem mass spectra	1577:1612	nearly identical tandem mass spectra	1577:1612	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	2	48	theme	single	484:489	arg1	structure					491:499	a single structure	482:499	a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers	482:641	However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers.					
32865981	0	49	theme	Trapped	47:53	arg1	Spectrometry					128:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	7	50	theme	identical	1584:1592	arg1	spectra					1606:1612	nearly identical tandem mass spectra	1577:1612	nearly identical tandem mass spectra	1577:1612	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	5	51	theme	mobility	1077:1084	arg1	power					1096:1100	a mobility resolving power	1075:1100	a mobility resolving power of ∼100	1075:1108	At a mobility resolving power of ∼100, 14 out of 22 structures showed more than one conformation.					
32865981	9	52	from	sources	2020:2026	arg1	analysis					1973:1980	analysis	1973:1980	analysis of ovalbumin N-glycans from different sources	1973:2026	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	9	52	from	sources	2020:2026	arg1	N-glycans					1995:2003	ovalbumin N-glycans	1985:2003	ovalbumin N-glycans from different sources	1985:2026	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	3	53	theme	glycans	704:710	arg1	sets					687:690	several sets	679:690	several sets	679:690	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	3	53	theme	glycans	704:710	arg1	glycans					704:710	isomeric glycans	695:710	isomeric glycans	695:710	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	9	54	from	level	2165:2169	arg1	variations					2114:2123	the batch-to-batch variations	2095:2123	the batch-to-batch variations in glycosylation profiles at the isomer level	2095:2169	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	9	54	from	level	2165:2169	arg1	profiles					2142:2149	glycosylation profiles	2128:2149	glycosylation profiles at the isomer level	2128:2169	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	2	55	theme	isomer	392:397	arg1	analysis					399:406	IM-based isomer analysis	383:406	IM-based isomer analysis	383:406	However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers.					
32865981	6	56	theme	structural	1316:1325	arg1	information					1327:1337	additional structural information	1305:1337	additional structural information that can differentiate isomers from conformers	1305:1384	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	8	57	theme	G-TIMS	1762:1767	arg1	analysis					1779:1786	G-TIMS EED MS/MS analysis	1762:1786	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin	1762:1830	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	1	58	theme	separate	269:276	arg1	isomers					278:284	separate isomers	269:284	separate isomers	269:284	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	9	59	theme	N-glycans	1995:2003	arg1	analysis					1973:1980	analysis	1973:1980	analysis of ovalbumin N-glycans from different sources	1973:2026	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	8	60	theme	MS/MS	1773:1777	arg1	analysis					1779:1786	G-TIMS EED MS/MS analysis	1762:1786	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin	1762:1830	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	2	61	theme	conformers	621:630	arg1	misinterpretation					600:616	misinterpretation	600:616	misinterpretation of conformers	600:630	However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers.					
32865981	9	62	theme	different	2010:2018	arg1	sources					2020:2026	different sources	2010:2026	different sources	2010:2026	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	8	63	theme	glycans	1800:1806	arg1	analysis					1779:1786	G-TIMS EED MS/MS analysis	1762:1786	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin	1762:1830	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	7	64	theme	glycan	1462:1467	arg1	mixtures					1469:1476	isomeric glycan mixtures	1453:1476	isomeric glycan mixtures following G-TIMS separation	1453:1504	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	3	65	theme	reduced	781:787	arg1	forms					789:793	both nonreduced and reduced forms	761:793	both nonreduced and reduced forms	761:793	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	1	66	theme	glycan	212:217	arg1	characterization					230:245	glycan structural characterization	212:245	glycan structural characterization due to its ability	212:264	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	9	67	from	analysis	1973:1980	arg1	sources					2020:2026	different sources	2010:2026	different sources	2010:2026	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	7	68	theme	ambiguity	1709:1717	arg1	evidence					1693:1700	minimal evidence	1685:1700	minimal evidence of any ambiguity resulting from the presence of conformers	1685:1759	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	9	69	theme	EED	2051:2053	arg1	approach					2061:2068	the G-TIMS EED MS/MS approach	2040:2068	the G-TIMS EED MS/MS approach	2040:2068	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	6	70	dep	need	1198:1201	arg1	acquire					1297:1303	acquire	1297:1303	to acquire additional structural information that can differentiate isomers from conformers	1294:1384	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	6	70	dep	need	1198:1201	arg1	use					1206:1208	use	1206:1208	to use IMS devices with high mobility resolving power for better separation of isomers	1203:1288	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	3	71	theme	nonreduced	766:775	arg1	forms					789:793	both nonreduced and reduced forms	761:793	both nonreduced and reduced forms	761:793	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	2	72	from	difficulty	525:534	arg1	separation					546:555	isomer separation	539:555	isomer separation	539:555	However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers.					
32865981	7	73	theme	MS/MS	1435:1439	arg1	analysis					1441:1448	Online electronic excitation dissociation (EED) MS/MS analysis	1387:1448	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation	1387:1504	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	8	74	theme	several	1846:1852	arg1	features					1863:1870	several mobility features	1846:1870	several mobility features previously thought to arise from isomeric structures	1846:1923	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	1	75	theme	Ion	142:144	arg1	spectrometry					160:171	Ion mobility-mass spectrometry	142:171	Ion mobility-mass spectrometry (IM-MS)	142:179	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	1	75	theme	Ion	142:144	arg1	IM-MS					174:178	IM-MS	174:178	IM-MS	174:178	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	8	76	attach	released	1808:1815	arg1	ovalbumin					1822:1830	ovalbumin	1822:1830	ovalbumin	1822:1830	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	8	76	attach	released	1808:1815	arg2	glycans					1800:1806	N-linked glycans	1791:1806	N-linked glycans released from ovalbumin	1791:1830	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	7	77	theme	minimal	1685:1691	arg1	evidence					1693:1700	minimal evidence	1685:1700	minimal evidence of any ambiguity resulting from the presence of conformers	1685:1759	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	6	78	theme	resolving	1241:1249	arg1	power					1251:1255	high mobility resolving power	1227:1255	high mobility resolving power for better separation of isomers	1227:1288	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	7	79	theme	isomers	1672:1678	arg1	identification					1654:1667	confident identification	1644:1667	confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers	1644:1759	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	4	80	theme	anomerism-induced	928:944	arg1	peak					962:965	anomerism-induced chromatographic peak	928:965	anomerism-induced chromatographic peak splitting	928:975	Notably, reducing-end reduction, commonly performed to remove anomerism-induced chromatographic peak splitting, did not eliminate the conformational heterogeneity of permethylated glycans in the gas phase.					
32865981	6	81	theme	high	1227:1230	arg1	power					1251:1255	high mobility resolving power	1227:1255	high mobility resolving power for better separation of isomers	1227:1288	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	3	82	theme	mobility	834:841	arg1	G-TIMS					857:862	G-TIMS	857:862	G-TIMS	857:862	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	3	82	theme	mobility	834:841	arg1	spectrometry					843:854	gated-trapped ion mobility spectrometry	816:854	gated-trapped ion mobility spectrometry (G-TIMS)	816:863	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	0	83	theme	Spectrometry-Electronic	68:90	arg1	Spectrometry					128:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	5	84	theme	∼100	1105:1108	arg1	power					1096:1100	a mobility resolving power	1075:1100	a mobility resolving power of ∼100	1075:1108	At a mobility resolving power of ∼100, 14 out of 22 structures showed more than one conformation.					
32865981	7	85	theme	electronic	1394:1403	arg1	analysis					1441:1448	Online electronic excitation dissociation (EED) MS/MS analysis	1387:1448	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation	1387:1504	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	3	86	theme	gated-trapped	816:828	arg1	G-TIMS					857:862	G-TIMS	857:862	G-TIMS	857:862	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	3	86	theme	gated-trapped	816:828	arg1	spectrometry					843:854	gated-trapped ion mobility spectrometry	816:854	gated-trapped ion mobility spectrometry (G-TIMS)	816:863	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	0	87	theme	Dissociation	103:114	arg1	Spectrometry					128:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	1	88	theme	structural	351:360	arg1	assignment					362:371	structural assignment	351:371	structural assignment	351:371	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	4	89	theme	permethylated	1032:1044	arg1	glycans					1046:1052	permethylated glycans	1032:1052	permethylated glycans	1032:1052	Notably, reducing-end reduction, commonly performed to remove anomerism-induced chromatographic peak splitting, did not eliminate the conformational heterogeneity of permethylated glycans in the gas phase.					
32865981	9	90	theme	glycosylation	2128:2140	arg1	profiles					2142:2149	glycosylation profiles	2128:2149	glycosylation profiles at the isomer level	2128:2169	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	0	91	theme	Mass	123:126	arg1	Spectrometry					128:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	8	92	theme	isomeric	1905:1912	arg1	structures					1914:1923	isomeric structures	1905:1923	isomeric structures	1905:1923	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	4	93	gly	heterogeneity	1015:1027	arg1	glycans					1046:1052	permethylated glycans	1032:1052	permethylated glycans	1032:1052	Notably, reducing-end reduction, commonly performed to remove anomerism-induced chromatographic peak splitting, did not eliminate the conformational heterogeneity of permethylated glycans in the gas phase.					
32865981	9	94	from	variations	2114:2123	arg1	level					2165:2169	the isomer level	2154:2169	the isomer level	2154:2169	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	9	94	from	variations	2114:2123	arg1	profiles					2142:2149	glycosylation profiles	2128:2149	glycosylation profiles at the isomer level	2128:2169	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	9	95	theme	isomer	2158:2163	arg1	level					2165:2169	the isomer level	2154:2169	the isomer level	2154:2169	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	4	96	theme	conformational	1000:1013	arg1	heterogeneity					1015:1027	the conformational heterogeneity	996:1027	the conformational heterogeneity of permethylated glycans in the gas phase	996:1069	Notably, reducing-end reduction, commonly performed to remove anomerism-induced chromatographic peak splitting, did not eliminate the conformational heterogeneity of permethylated glycans in the gas phase.					
32865981	2	97	theme	conformations	465:477	arg1	presence					434:441	the presence	430:441	the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers	430:641	However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers.					
32865981	0	98	theme	Isomeric	27:34	arg1	Glycans					36:42	Isomeric Glycans	27:42	Isomeric Glycans	27:42	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	1	99	theme	cross	308:312	arg1	section					314:320	collision cross section	298:320	collision cross section (CCS) values that facilitate structural assignment	298:371	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	1	99	theme	cross	308:312	arg1	CCS					323:325	CCS	323:325	CCS	323:325	Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment.					
32865981	8	100	theme	single	1946:1951	arg1	structure					1953:1961	a single structure	1944:1961	a single structure	1944:1961	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	2	101	theme	multiple	446:453	arg1	conformations					465:477	multiple gas-phase conformations	446:477	multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers	446:641	However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers.					
32865981	9	102	theme	confident	2177:2185	arg1	assignment					2187:2196	confident assignment	2177:2196	confident assignment of each isomeric structure	2177:2223	Finally, analysis of ovalbumin N-glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.					
32865981	6	103	from	conformers	1375:1384	arg1	isomers					1362:1368	isomers	1362:1368	isomers from conformers	1362:1384	These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers.					
32865981	8	104	link	N-linked	1791:1798	arg1	glycans					1800:1806	N-linked glycans	1791:1806	N-linked glycans released from ovalbumin	1791:1830	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	2	105	theme	structure	491:499	arg1	conformations					465:477	multiple gas-phase conformations	446:477	multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers	446:641	However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers.					
32865981	3	106	theme	mobility	658:665	arg1	behavior					667:674	the ion mobility behavior	650:674	the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms,	650:794	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	0	107	theme	Ion	55:57	arg1	Spectrometry					128:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry	47:139	Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.					
32865981	4	108	theme	gas	1061:1063	arg1	phase					1065:1069	the gas phase	1057:1069	the gas phase	1057:1069	Notably, reducing-end reduction, commonly performed to remove anomerism-induced chromatographic peak splitting, did not eliminate the conformational heterogeneity of permethylated glycans in the gas phase.					
32865981	3	109	theme	isomeric	695:702	arg1	glycans					704:710	isomeric glycans	695:710	isomeric glycans	695:710	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	7	110	theme	tandem	1594:1599	arg1	spectra					1606:1612	nearly identical tandem mass spectra	1577:1612	nearly identical tandem mass spectra	1577:1612	Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers.					
32865981	3	111	theme	sets	687:690	arg1	behavior					667:674	the ion mobility behavior	650:674	the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms,	650:794	Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS).					
32865981	2	112	theme	IM-based	383:390	arg1	analysis					399:406	IM-based isomer analysis	383:406	IM-based isomer analysis	383:406	However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers.					
32865981	8	113	theme	EED	1769:1771	arg1	analysis					1779:1786	G-TIMS EED MS/MS analysis	1762:1786	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin	1762:1830	G-TIMS EED MS/MS analysis of N-linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure.					
32865981	4	114	from	heterogeneity	1015:1027	arg1	phase					1065:1069	the gas phase	1057:1069	the gas phase	1057:1069	Notably, reducing-end reduction, commonly performed to remove anomerism-induced chromatographic peak splitting, did not eliminate the conformational heterogeneity of permethylated glycans in the gas phase.					
33080533	0	0	theme	isomer	36:41	arg1	separation					43:52	fucosylated N-glycan isomer separation	15:52	fucosylated N-glycan isomer separation	15:52	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	6	1	theme	μL/min	1084:1089	arg1	rate					1096:1099	a 250 μL/min flow rate	1078:1099	a 250 μL/min flow rate for native glycan analysis	1078:1126	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	1	2	theme	glycan	270:275	arg1	isomers					277:283	distinct glycan isomers	261:283	distinct glycan isomers	261:283	Due to the heterogeneous and isomeric nature of glycans, the development of an advanced separation of distinct glycan isomers is essential for glycan research and application.					
33080533	9	3	theme	ions	1486:1489	arg1	intensities					1456:1466	The intensities	1452:1466	The intensities of the diagnostic ions of synthetic isomers	1452:1510	The intensities of the diagnostic ions of synthetic isomers were used to identify each isomer of the fucosylated glycan.					
33080533	5	4	theme	PGC	813:815	arg1	column					817:822	the PGC column	809:822	the PGC column	809:822	When the temperature of the PGC column was increased from 25 to 190 °C, the liquid chromatography separation power of the nonreduced dextran ladder significantly increased.					
33080533	5	5	theme	chromatography	868:881	arg1	power					894:898	the liquid chromatography separation power	857:898	the liquid chromatography separation power of the nonreduced dextran ladder	857:931	When the temperature of the PGC column was increased from 25 to 190 °C, the liquid chromatography separation power of the nonreduced dextran ladder significantly increased.					
33080533	8	6	theme	spectrometry-based	1245:1262	arg1	sequencing					1264:1273	mass spectrometry-based sequencing	1240:1273	mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens	1240:1349	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	1	7	theme	glycans	207:213	arg1	nature					197:202	the heterogeneous and isomeric nature	166:202	nature	197:202	Due to the heterogeneous and isomeric nature of glycans, the development of an advanced separation of distinct glycan isomers is essential for glycan research and application.					
33080533	0	8	theme	chromatography-mass	125:143	arg1	spectrometry					145:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	3	9	theme	glycan	577:582	arg1	isomers					584:590	glycan isomers	577:590	glycan isomers	577:590	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	6	10	theme	UHT-PGC	979:985	arg1	column					987:992	the UHT-PGC column	975:992	the UHT-PGC column	975:992	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	2	11	from	190 °C	502:507	arg1	oven					535:538	a custom-built heating oven	512:538	a custom-built heating oven	512:538	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
33080533	8	12	used	used	1355:1358	arg2	sequencing					1264:1273	mass spectrometry-based sequencing	1240:1273	mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens	1240:1349	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	8	13	theme	specific	1287:1294	arg1	ions					1307:1310	specific diagnostic ions	1287:1310	specific diagnostic ions from the four synthetic Lewis antigens	1287:1349	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	10	14	theme	A	1620:1620	arg1	residues					1628:1635	terminal Lewis A and X residues	1605:1635	terminal Lewis A and X residues	1605:1635	The results clearly showed that terminal Lewis A and X residues were in the 3- and 6-arms of N-glycan, respectively.					
33080533	0	15	theme	N-glycan	27:34	arg1	separation					43:52	fucosylated N-glycan isomer separation	15:52	fucosylated N-glycan isomer separation	15:52	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	9	16	theme	diagnostic	1475:1484	arg1	ions					1486:1489	the diagnostic ions	1471:1489	the diagnostic ions of synthetic isomers	1471:1510	The intensities of the diagnostic ions of synthetic isomers were used to identify each isomer of the fucosylated glycan.					
33080533	10	17	theme	terminal	1605:1612	arg1	residues					1628:1635	terminal Lewis A and X residues	1605:1635	terminal Lewis A and X residues	1605:1635	The results clearly showed that terminal Lewis A and X residues were in the 3- and 6-arms of N-glycan, respectively.					
33080533	8	18	theme	diagnostic	1435:1444	arg1	ions					1446:1449	diagnostic ions	1435:1449	diagnostic ions	1435:1449	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	3	19	theme	liquid	647:652	arg1	spectrometry					674:685	ultrahigh temperature PGC liquid chromatography mass spectrometry	621:685	ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS)	621:700	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	3	19	theme	liquid	647:652	arg1	UHT-PGC-LCMS					688:699	UHT-PGC-LCMS	688:699	UHT-PGC-LCMS	688:699	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	9	20	theme	isomers	1504:1510	arg1	ions					1486:1489	the diagnostic ions	1471:1489	the diagnostic ions of synthetic isomers	1471:1510	The intensities of the diagnostic ions of synthetic isomers were used to identify each isomer of the fucosylated glycan.					
33080533	9	21	used	used	1517:1520	arg2	intensities					1456:1466	The intensities	1452:1466	The intensities of the diagnostic ions of synthetic isomers	1452:1510	The intensities of the diagnostic ions of synthetic isomers were used to identify each isomer of the fucosylated glycan.					
33080533	2	22	theme	custom-built	514:525	arg1	oven					535:538	a custom-built heating oven	512:538	a custom-built heating oven	512:538	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
33080533	4	23	theme	hydrolyzed	716:725	arg1	dextran					727:733	A nonreduced hydrolyzed dextran	703:733	A nonreduced hydrolyzed dextran	703:733	A nonreduced hydrolyzed dextran was applied to verify the performance of UHT-PGC.					
33080533	0	24	theme	ultrahigh	62:70	arg1	temperature					79:89	an ultrahigh column temperature	59:89	an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry	59:156	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	3	25	theme	temperature	631:641	arg1	spectrometry					674:685	ultrahigh temperature PGC liquid chromatography mass spectrometry	621:685	ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS)	621:700	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	3	25	theme	temperature	631:641	arg1	UHT-PGC-LCMS					688:699	UHT-PGC-LCMS	688:699	UHT-PGC-LCMS	688:699	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	6	26	theme	gradient	1026:1033	arg1	elution					1035:1041	gradient elution	1026:1041	gradient elution	1026:1041	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	5	27	theme	dextran	918:924	arg1	ladder					926:931	the nonreduced dextran ladder	903:931	the nonreduced dextran ladder	903:931	When the temperature of the PGC column was increased from 25 to 190 °C, the liquid chromatography separation power of the nonreduced dextran ladder significantly increased.					
33080533	0	28	theme	porous	94:99	arg1	spectrometry					145:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	6	29	theme	high	1002:1005	arg1	capacity					1012:1019	its high peak capacity	998:1019	its high peak capacity with gradient elution	998:1041	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	29	theme	high	1002:1005	arg1	advantage					962:970	The advantage	958:970	The advantage of the UHT-PGC column	958:992	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	0	30	theme	carbon	111:116	arg1	spectrometry					145:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	0	31	theme	separation	43:52	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.	0:157	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	8	32	from	antigens	1342:1349	arg1	ions					1307:1310	specific diagnostic ions	1287:1310	specific diagnostic ions from the four synthetic Lewis antigens	1287:1349	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	8	33	dep	sequencing	1264:1273	arg1	generate					1278:1285	generate	1278:1285	to generate specific diagnostic ions from the four synthetic Lewis antigens	1275:1349	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	2	34	theme	PGC	386:388	arg1	chromatography					391:404	porous graphite carbon (PGC) chromatography	362:404	porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides	362:452	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
33080533	9	35	theme	glycan	1565:1570	arg1	isomer					1539:1544	each isomer	1534:1544	each isomer of the fucosylated glycan	1534:1570	The intensities of the diagnostic ions of synthetic isomers were used to identify each isomer of the fucosylated glycan.					
33080533	2	36	theme	oligosaccharides	437:452	arg1	separation					414:423	the separation	410:423	the separation of isomeric oligosaccharides	410:452	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
33080533	10	37	dep	3-	1649:1650	arg1	the					1645:1647	the	1645:1647	the	1645:1647	The results clearly showed that terminal Lewis A and X residues were in the 3- and 6-arms of N-glycan, respectively.					
33080533	0	38	theme	fucosylated	15:25	arg1	separation					43:52	fucosylated N-glycan isomer separation	15:52	fucosylated N-glycan isomer separation	15:52	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	1	39	theme	glycan	302:307	arg1	research					309:316	glycan research	302:316	glycan research	302:316	Due to the heterogeneous and isomeric nature of glycans, the development of an advanced separation of distinct glycan isomers is essential for glycan research and application.					
33080533	8	40	theme	synthetic	1326:1334	arg1	antigens					1342:1349	the four synthetic Lewis antigens	1317:1349	the four synthetic Lewis antigens	1317:1349	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	8	41	theme	relative	1401:1408	arg1	RIR					1427:1429	RIR	1427:1429	RIR	1427:1429	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	8	41	theme	relative	1401:1408	arg1	ratio					1420:1424	the relative intensity ratio	1397:1424	the relative intensity ratio (RIR) of diagnostic ions	1397:1449	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	1	42	theme	isomeric	188:195	arg1	nature					197:202	the heterogeneous and isomeric nature	166:202	nature	197:202	Due to the heterogeneous and isomeric nature of glycans, the development of an advanced separation of distinct glycan isomers is essential for glycan research and application.					
33080533	7	43	theme	Lewis	1144:1148	arg1	isomers					1158:1164	Four synthetic Lewis antigen isomers	1129:1164	Four synthetic Lewis antigen isomers	1129:1164	Four synthetic Lewis antigen isomers were used to elucidate the separation effectiveness in UHT-PGC.					
33080533	2	44	theme	chemical	475:482	arg1	derivatization					484:497	chemical derivatization	475:497	chemical derivatization	475:497	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
33080533	9	45	gly	fucosylated	1553:1563	arg1	glycan					1565:1570	the fucosylated glycan	1549:1570	the fucosylated glycan	1549:1570	The intensities of the diagnostic ions of synthetic isomers were used to identify each isomer of the fucosylated glycan.					
33080533	3	46	theme	mass	669:672	arg1	spectrometry					674:685	ultrahigh temperature PGC liquid chromatography mass spectrometry	621:685	ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS)	621:700	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	3	46	theme	mass	669:672	arg1	UHT-PGC-LCMS					688:699	UHT-PGC-LCMS	688:699	UHT-PGC-LCMS	688:699	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	6	47	theme	glycan	1112:1117	arg1	analysis					1119:1126	native glycan analysis	1105:1126	native glycan analysis	1105:1126	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	0	48	with	Enhancement	0:10	arg1	temperature					79:89	an ultrahigh column temperature	59:89	an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry	59:156	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	10	49	theme	N-glycan	1666:1673	arg1	3-					1649:1650	3-	1649:1650	3-	1649:1650	The results clearly showed that terminal Lewis A and X residues were in the 3- and 6-arms of N-glycan, respectively.					
33080533	10	49	theme	N-glycan	1666:1673	arg1	N-glycan					1666:1673	N-glycan	1666:1673	N-glycan	1666:1673	The results clearly showed that terminal Lewis A and X residues were in the 3- and 6-arms of N-glycan, respectively.					
33080533	5	50	dep	190	849:851	arg1	to					846:847	to	846:847	to	846:847	When the temperature of the PGC column was increased from 25 to 190 °C, the liquid chromatography separation power of the nonreduced dextran ladder significantly increased.					
33080533	2	51	theme	carbon	378:383	arg1	chromatography					391:404	porous graphite carbon (PGC) chromatography	362:404	porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides	362:452	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
33080533	1	52	theme	separation	247:256	arg1	essential					288:296	essential	288:296	essential	288:296	Due to the heterogeneous and isomeric nature of glycans, the development of an advanced separation of distinct glycan isomers is essential for glycan research and application.					
33080533	1	52	theme	separation	247:256	arg1	development					220:230	the development	216:230	the development of an advanced separation of distinct glycan isomers	216:283	Due to the heterogeneous and isomeric nature of glycans, the development of an advanced separation of distinct glycan isomers is essential for glycan research and application.					
33080533	6	53	theme	flow	1091:1094	arg1	rate					1096:1099	a 250 μL/min flow rate	1078:1099	a 250 μL/min flow rate for native glycan analysis	1078:1126	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	2	54	theme	porous	362:367	arg1	chromatography					391:404	porous graphite carbon (PGC) chromatography	362:404	porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides	362:452	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
33080533	1	55	theme	distinct	261:268	arg1	isomers					277:283	distinct glycan isomers	261:283	distinct glycan isomers	261:283	Due to the heterogeneous and isomeric nature of glycans, the development of an advanced separation of distinct glycan isomers is essential for glycan research and application.					
33080533	6	56	theme	250	1080:1082	arg1	μL/min					1084:1089	μL/min	1084:1089	μL/min	1084:1089	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	57	from	6700 psi	1064:1071	arg1	capacity					1012:1019	its high peak capacity	998:1019	its high peak capacity with gradient elution	998:1041	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	57	from	6700 psi	1064:1071	arg1	advantage					962:970	The advantage	958:970	The advantage of the UHT-PGC column	958:992	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	57	from	6700 psi	1064:1071	arg1	10 min					1046:1051	10 min	1046:1051	10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis	1046:1126	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	3	58	theme	fine	558:561	arg1	structures					563:572	the fine structures	554:572	the fine structures of glycan isomers	554:590	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	1	59	theme	isomers	277:283	arg1	separation					247:256	an advanced separation	235:256	an advanced separation of distinct glycan isomers	235:283	Due to the heterogeneous and isomeric nature of glycans, the development of an advanced separation of distinct glycan isomers is essential for glycan research and application.					
33080533	5	60	theme	nonreduced	907:916	arg1	ladder					926:931	the nonreduced dextran ladder	903:931	the nonreduced dextran ladder	903:931	When the temperature of the PGC column was increased from 25 to 190 °C, the liquid chromatography separation power of the nonreduced dextran ladder significantly increased.					
33080533	5	61	theme	liquid	861:866	arg1	power					894:898	the liquid chromatography separation power	857:898	the liquid chromatography separation power of the nonreduced dextran ladder	857:931	When the temperature of the PGC column was increased from 25 to 190 °C, the liquid chromatography separation power of the nonreduced dextran ladder significantly increased.					
33080533	3	62	theme	isomers	584:590	arg1	structures					563:572	the fine structures	554:572	the fine structures of glycan isomers	554:590	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	5	63	theme	separation	883:892	arg1	power					894:898	the liquid chromatography separation power	857:898	the liquid chromatography separation power of the nonreduced dextran ladder	857:931	When the temperature of the PGC column was increased from 25 to 190 °C, the liquid chromatography separation power of the nonreduced dextran ladder significantly increased.					
33080533	8	64	theme	mass	1240:1243	arg1	sequencing					1264:1273	mass spectrometry-based sequencing	1240:1273	mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens	1240:1349	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	6	65	from	°C	1060:1061	arg1	capacity					1012:1019	its high peak capacity	998:1019	its high peak capacity with gradient elution	998:1041	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	65	from	°C	1060:1061	arg1	advantage					962:970	The advantage	958:970	The advantage of the UHT-PGC column	958:992	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	65	from	°C	1060:1061	arg1	10 min					1046:1051	10 min	1046:1051	10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis	1046:1126	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	66	theme	column	987:992	arg1	capacity					1012:1019	its high peak capacity	998:1019	its high peak capacity with gradient elution	998:1041	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	66	theme	column	987:992	arg1	advantage					962:970	The advantage	958:970	The advantage of the UHT-PGC column	958:992	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	8	67	theme	ions	1446:1449	arg1	RIR					1427:1429	RIR	1427:1429	RIR	1427:1429	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	8	67	theme	ions	1446:1449	arg1	ratio					1420:1424	the relative intensity ratio	1397:1424	the relative intensity ratio (RIR) of diagnostic ions	1397:1449	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	10	68	theme	Lewis	1614:1618	arg1	A					1620:1620	Lewis A	1614:1620	Lewis A	1614:1620	The results clearly showed that terminal Lewis A and X residues were in the 3- and 6-arms of N-glycan, respectively.					
33080533	10	69	theme	X	1626:1626	arg1	residues					1628:1635	terminal Lewis A and X residues	1605:1635	terminal Lewis A and X residues	1605:1635	The results clearly showed that terminal Lewis A and X residues were in the 3- and 6-arms of N-glycan, respectively.					
33080533	5	70	theme	ladder	926:931	arg1	power					894:898	the liquid chromatography separation power	857:898	the liquid chromatography separation power of the nonreduced dextran ladder	857:931	When the temperature of the PGC column was increased from 25 to 190 °C, the liquid chromatography separation power of the nonreduced dextran ladder significantly increased.					
33080533	7	71	from	effectiveness	1204:1216	arg1	UHT-PGC					1221:1227	UHT-PGC	1221:1227	UHT-PGC	1221:1227	Four synthetic Lewis antigen isomers were used to elucidate the separation effectiveness in UHT-PGC.					
33080533	8	72	theme	diagnostic	1296:1305	arg1	ions					1307:1310	specific diagnostic ions	1287:1310	specific diagnostic ions from the four synthetic Lewis antigens	1287:1349	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	3	73	theme	PGC	643:645	arg1	spectrometry					674:685	ultrahigh temperature PGC liquid chromatography mass spectrometry	621:685	ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS)	621:700	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	3	73	theme	PGC	643:645	arg1	UHT-PGC-LCMS					688:699	UHT-PGC-LCMS	688:699	UHT-PGC-LCMS	688:699	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	9	74	theme	synthetic	1494:1502	arg1	isomers					1504:1510	synthetic isomers	1494:1510	synthetic isomers	1494:1510	The intensities of the diagnostic ions of synthetic isomers were used to identify each isomer of the fucosylated glycan.					
33080533	0	75	theme	column	72:77	arg1	temperature					79:89	an ultrahigh column temperature	59:89	an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry	59:156	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	3	76	theme	ultrahigh	621:629	arg1	spectrometry					674:685	ultrahigh temperature PGC liquid chromatography mass spectrometry	621:685	ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS)	621:700	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	3	76	theme	ultrahigh	621:629	arg1	UHT-PGC-LCMS					688:699	UHT-PGC-LCMS	688:699	UHT-PGC-LCMS	688:699	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	6	77	with	capacity	1012:1019	arg1	elution					1035:1041	gradient elution	1026:1041	gradient elution	1026:1041	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	4	78	theme	nonreduced	705:714	arg1	dextran					727:733	A nonreduced hydrolyzed dextran	703:733	A nonreduced hydrolyzed dextran	703:733	A nonreduced hydrolyzed dextran was applied to verify the performance of UHT-PGC.					
33080533	7	79	theme	separation	1193:1202	arg1	effectiveness					1204:1216	the separation effectiveness	1189:1216	the separation effectiveness in UHT-PGC	1189:1227	Four synthetic Lewis antigen isomers were used to elucidate the separation effectiveness in UHT-PGC.					
33080533	1	80	theme	heterogeneous	170:182	arg1	nature					197:202	the heterogeneous and isomeric nature	166:202	nature	197:202	Due to the heterogeneous and isomeric nature of glycans, the development of an advanced separation of distinct glycan isomers is essential for glycan research and application.					
33080533	2	81	used	utilized	353:360	arg2	we					350:351	we	350:351	we	350:351	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
33080533	8	82	theme	Lewis	1336:1340	arg1	antigens					1342:1349	the four synthetic Lewis antigens	1317:1349	the four synthetic Lewis antigens	1317:1349	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	0	83	theme	graphitic	101:109	arg1	spectrometry					145:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	6	84	theme	peak	1007:1010	arg1	capacity					1012:1019	its high peak capacity	998:1019	its high peak capacity with gradient elution	998:1041	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	84	theme	peak	1007:1010	arg1	advantage					962:970	The advantage	958:970	The advantage of the UHT-PGC column	958:992	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	0	85	theme	liquid	118:123	arg1	spectrometry					145:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	2	86	theme	heating	527:533	arg1	oven					535:538	a custom-built heating oven	512:538	a custom-built heating oven	512:538	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
33080533	9	87	theme	fucosylated	1553:1563	arg1	glycan					1565:1570	the fucosylated glycan	1549:1570	the fucosylated glycan	1549:1570	The intensities of the diagnostic ions of synthetic isomers were used to identify each isomer of the fucosylated glycan.					
33080533	6	88	from	capacity	1012:1019	arg1	°C					1060:1061	190 °C	1056:1061	190 °C	1056:1061	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	88	from	capacity	1012:1019	arg1	rate					1096:1099	a 250 μL/min flow rate	1078:1099	a 250 μL/min flow rate for native glycan analysis	1078:1126	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	88	from	capacity	1012:1019	arg1	6700 psi					1064:1071	6700 psi	1064:1071	6700 psi	1064:1071	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	88	from	capacity	1012:1019	arg1	10 min					1046:1051	10 min	1046:1051	10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis	1046:1126	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	8	89	theme	isomeric	1371:1378	arg1	glycans					1380:1386	isomeric glycans	1371:1386	isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions	1371:1449	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	0	90	from	temperature	79:89	arg1	spectrometry					145:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	porous graphitic carbon liquid chromatography-mass spectrometry	94:156	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	7	91	theme	antigen	1150:1156	arg1	isomers					1158:1164	Four synthetic Lewis antigen isomers	1129:1164	Four synthetic Lewis antigen isomers	1129:1164	Four synthetic Lewis antigen isomers were used to elucidate the separation effectiveness in UHT-PGC.					
33080533	2	92	theme	isomeric	428:435	arg1	oligosaccharides					437:452	isomeric oligosaccharides	428:452	isomeric oligosaccharides	428:452	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
33080533	4	93	theme	UHT-PGC	776:782	arg1	performance					761:771	the performance	757:771	the performance of UHT-PGC	757:782	A nonreduced hydrolyzed dextran was applied to verify the performance of UHT-PGC.					
33080533	7	94	theme	synthetic	1134:1142	arg1	isomers					1158:1164	Four synthetic Lewis antigen isomers	1129:1164	Four synthetic Lewis antigen isomers	1129:1164	Four synthetic Lewis antigen isomers were used to elucidate the separation effectiveness in UHT-PGC.					
33080533	7	95	used	used	1171:1174	arg2	isomers					1158:1164	Four synthetic Lewis antigen isomers	1129:1164	Four synthetic Lewis antigen isomers	1129:1164	Four synthetic Lewis antigen isomers were used to elucidate the separation effectiveness in UHT-PGC.					
33080533	3	96	theme	chromatography	654:667	arg1	spectrometry					674:685	ultrahigh temperature PGC liquid chromatography mass spectrometry	621:685	ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS)	621:700	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	3	96	theme	chromatography	654:667	arg1	UHT-PGC-LCMS					688:699	UHT-PGC-LCMS	688:699	UHT-PGC-LCMS	688:699	Furthermore, the fine structures of glycan isomers could be identified by using ultrahigh temperature PGC liquid chromatography mass spectrometry (UHT-PGC-LCMS).					
33080533	5	97	theme	column	817:822	arg1	temperature					794:804	the temperature	790:804	the temperature of the PGC column	790:822	When the temperature of the PGC column was increased from 25 to 190 °C, the liquid chromatography separation power of the nonreduced dextran ladder significantly increased.					
33080533	0	98	gly	fucosylated	15:25	arg1	separation					43:52	fucosylated N-glycan isomer separation	15:52	fucosylated N-glycan isomer separation	15:52	Enhancement of fucosylated N-glycan isomer separation with an ultrahigh column temperature in porous graphitic carbon liquid chromatography-mass spectrometry.					
33080533	8	99	theme	intensity	1410:1418	arg1	RIR					1427:1429	RIR	1427:1429	RIR	1427:1429	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	8	99	theme	intensity	1410:1418	arg1	ratio					1420:1424	the relative intensity ratio	1397:1424	the relative intensity ratio (RIR) of diagnostic ions	1397:1449	Moreover, mass spectrometry-based sequencing to generate specific diagnostic ions from the four synthetic Lewis antigens was used to predict isomeric glycans based on the relative intensity ratio (RIR) of diagnostic ions.					
33080533	6	100	theme	native	1105:1110	arg1	analysis					1119:1126	native glycan analysis	1105:1126	native glycan analysis	1105:1126	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	1	101	theme	advanced	238:245	arg1	separation					247:256	an advanced separation	235:256	an advanced separation of distinct glycan isomers	235:283	Due to the heterogeneous and isomeric nature of glycans, the development of an advanced separation of distinct glycan isomers is essential for glycan research and application.					
33080533	6	102	from	rate	1096:1099	arg1	capacity					1012:1019	its high peak capacity	998:1019	its high peak capacity with gradient elution	998:1041	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	102	from	rate	1096:1099	arg1	advantage					962:970	The advantage	958:970	The advantage of the UHT-PGC column	958:992	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	6	102	from	rate	1096:1099	arg1	10 min					1046:1051	10 min	1046:1051	10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis	1046:1126	The advantage of the UHT-PGC column was its high peak capacity with gradient elution in 10 min at 190 °C, 6700 psi, and a 250 μL/min flow rate for native glycan analysis.					
33080533	2	103	theme	graphite	369:376	arg1	chromatography					391:404	porous graphite carbon (PGC) chromatography	362:404	porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides	362:452	In this study, we utilized porous graphite carbon (PGC) chromatography for the separation of isomeric oligosaccharides without reduction or chemical derivatization at 190 °C in a custom-built heating oven.					
34364435	7	0	theme	robust	1222:1227	arg1	approach					1243:1250	a robust computational approach	1220:1250	a robust computational approach for the determination of glycan diversity within different complex biological systems	1220:1336	Combined, these results show that this strategy is a robust computational approach for the determination of glycan diversity within different complex biological systems.					
34364435	7	0	theme	robust	1222:1227	arg1	strategy					1208:1215	this strategy	1203:1215	this strategy	1203:1215	Combined, these results show that this strategy is a robust computational approach for the determination of glycan diversity within different complex biological systems.					
34364435	6	1	theme	Acinetobacter	1054:1066	arg1	baumannii					1068:1076	Acinetobacter baumannii ATCC17978	1054:1086	Acinetobacter baumannii ATCC17978	1054:1086	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	0	2	theme	library	73:79	arg1	construction					31:42	the construction	27:42	the construction of a species-specific glycan library	27:79	An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses.					
34364435	5	3	theme	software	793:800	arg1	tool					802:805	the software tool	789:805	the software tool	789:805	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	1	4	theme	-based	161:166	arg1	strategies					168:177	Mass spectrometry (MS)-based strategies	139:177	Mass spectrometry (MS)-based strategies	139:177	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	0	5	theme	spectrometry-based	90:107	arg1	analyses					129:136	mass spectrometry-based intact glycopeptide analyses	85:136	mass spectrometry-based intact glycopeptide analyses	85:136	An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses.					
34364435	2	6	theme	mammals	351:357	arg1	glycans					340:346	normal glycans	333:346	normal glycans of mammals	333:357	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	2	7	theme	glycan	298:303	arg1	lists					310:314	Most glycan mass lists	293:314	Most glycan mass lists	293:314	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	2	8	theme	mass	305:308	arg1	lists					310:314	Most glycan mass lists	293:314	Most glycan mass lists	293:314	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	0	9	theme	mass	85:88	arg1	analyses					129:136	mass spectrometry-based intact glycopeptide analyses	85:136	mass spectrometry-based intact glycopeptide analyses	85:136	An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses.					
34364435	6	10	gly	N-glycosylation	1001:1015	arg1	O-glycosylation					1035:1049	O-glycosylation	1035:1049	O-glycosylation of Acinetobacter baumannii ATCC17978	1035:1086	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	6	10	gly	N-glycosylation	1001:1015	arg1	roots					1025:1029	rice roots	1020:1029	rice roots	1020:1029	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	2	11	theme	Most	293:296	arg1	lists					310:314	Most glycan mass lists	293:314	Most glycan mass lists	293:314	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	1	12	theme	intact	271:276	arg1	glycopeptides					278:290	intact glycopeptides	271:290	intact glycopeptides	271:290	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	0	13	theme	glycopeptide	116:127	arg1	analyses					129:136	mass spectrometry-based intact glycopeptide analyses	85:136	mass spectrometry-based intact glycopeptide analyses	85:136	An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses.					
34364435	6	14	theme	glycopeptides	1141:1153	arg1	identification					1104:1117	the identification	1100:1117	the identification of 296 and 145 intact glycopeptides respectively	1100:1166	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	5	15	theme	monoisotope	853:863	arg1	mass					865:868	monoisotope mass	853:868	monoisotope mass	853:868	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	2	16	located	observed	485:492	arg1	species					503:509	other species	497:509	other species	497:509	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	2	16	located	observed	485:492	arg2	events					478:483	unusual glycosylation events	456:483	unusual glycosylation events observed in other species	456:509	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	4	17	theme	computational	692:704	arg1	tool					706:709	a computational tool	690:709	a computational tool	690:709	We developed a computational tool called LibGlycan, which could process the different formats of glycans.					
34364435	5	18	theme	average	871:877	arg1	mass					879:882	average mass	871:882	average mass	871:882	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	0	19	theme	intact	109:114	arg1	analyses					129:136	mass spectrometry-based intact glycopeptide analyses	85:136	mass spectrometry-based intact glycopeptide analyses	85:136	An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses.					
34364435	2	20	contain	contain	363:369	arg1	lists					310:314	Most glycan mass lists	293:314	Most glycan mass lists	293:314	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	2	20	contain	contain	363:369	arg2	monosaccharides					379:393	limited monosaccharides	371:393	limited monosaccharides	371:393	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	5	21	theme	glycan	819:824	arg1	library					826:832	a glycan library	817:832	a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software	817:957	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	6	22	theme	roots	1025:1029	arg1	N-glycosylation					1001:1015	the N-glycosylation	997:1015	the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978	997:1086	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	3	23	from	papers	669:674	arg1	construction					571:582	the construction	567:582	the construction of a species-specific glycan mass list from glycan structure databases and published papers	567:674	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	3	23	from	papers	669:674	arg1	list					618:621	a species-specific glycan mass list	587:621	a species-specific glycan mass list from glycan structure databases and published papers	587:674	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	0	24	theme	integrated	3:12	arg1	strategy					14:21	An integrated strategy	0:21	An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses.	0:137	An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses.					
34364435	3	25	theme	integrated	529:538	arg1	strategy					540:547	an integrated strategy	526:547	an integrated strategy	526:547	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	7	26	theme	computational	1229:1241	arg1	approach					1243:1250	a robust computational approach	1220:1250	a robust computational approach for the determination of glycan diversity within different complex biological systems	1220:1336	Combined, these results show that this strategy is a robust computational approach for the determination of glycan diversity within different complex biological systems.					
34364435	7	26	theme	computational	1229:1241	arg1	strategy					1208:1215	this strategy	1203:1215	this strategy	1203:1215	Combined, these results show that this strategy is a robust computational approach for the determination of glycan diversity within different complex biological systems.					
34364435	3	27	from	databases	645:653	arg1	construction					571:582	the construction	567:582	the construction of a species-specific glycan mass list from glycan structure databases and published papers	567:674	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	3	27	from	databases	645:653	arg1	list					618:621	a species-specific glycan mass list	587:621	a species-specific glycan mass list from glycan structure databases and published papers	587:674	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	1	28	theme	related	183:189	arg1	tools					200:204	related software tools	183:204	related software tools	183:204	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	2	29	theme	unusual	456:462	arg1	events					478:483	unusual glycosylation events	456:483	unusual glycosylation events observed in other species	456:509	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	1	30	theme	glycopeptides	278:290	arg1	analysis					259:266	the analysis	255:266	the analysis of intact glycopeptides	255:290	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	4	31	theme	different	753:761	arg1	formats					763:769	the different formats	749:769	the different formats of glycans	749:780	We developed a computational tool called LibGlycan, which could process the different formats of glycans.					
34364435	7	32	theme	biological	1319:1328	arg1	systems					1330:1336	different complex biological systems	1301:1336	different complex biological systems	1301:1336	Combined, these results show that this strategy is a robust computational approach for the determination of glycan diversity within different complex biological systems.					
34364435	1	33	theme	software	191:198	arg1	tools					200:204	related software tools	183:204	related software tools	183:204	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	2	34	attach	derived	320:326	arg1	glycans					340:346	normal glycans	333:346	normal glycans of mammals	333:357	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	2	34	attach	derived	320:326	arg2	lists					310:314	Most glycan mass lists	293:314	Most glycan mass lists	293:314	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	4	35	theme	glycans	774:780	arg1	formats					763:769	the different formats	749:769	the different formats of glycans	749:780	We developed a computational tool called LibGlycan, which could process the different formats of glycans.					
34364435	2	36	theme	throughput	434:443	arg1	studies					445:451	high throughput studies	429:451	high throughput studies of unusual glycosylation events observed in other species	429:509	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	5	37	theme	Byonic	943:948	arg1	software					950:957	Byonic software	943:957	Byonic software	943:957	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	5	38	contain	contained	839:847	arg1	library					826:832	a glycan library	817:832	a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software	817:957	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	5	38	contain	contained	839:847	arg2	list					923:926	the monoisotope mass, average mass, isotope distribution, and glycan mass list	849:926	the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software	849:957	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	0	39	gly	glycopeptide	116:127	arg2	glycopeptide					116:127	mass spectrometry-based intact glycopeptide analyses	85:136	mass spectrometry-based intact glycopeptide analyses	85:136	An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses.					
34364435	2	40	theme	high	429:432	arg1	studies					445:451	high throughput studies	429:451	high throughput studies of unusual glycosylation events observed in other species	429:509	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	2	41	theme	other	497:501	arg1	species					503:509	other species	497:509	other species	497:509	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	7	42	theme	complex	1311:1317	arg1	systems					1330:1336	different complex biological systems	1301:1336	different complex biological systems	1301:1336	Combined, these results show that this strategy is a robust computational approach for the determination of glycan diversity within different complex biological systems.					
34364435	6	43	gly	O-glycosylation	1035:1049	arg1	baumannii					1068:1076	Acinetobacter baumannii ATCC17978	1054:1086	Acinetobacter baumannii ATCC17978	1054:1086	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	5	44	theme	mass	865:868	arg1	list					923:926	the monoisotope mass, average mass, isotope distribution, and glycan mass list	849:926	the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software	849:957	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	3	45	theme	mass	613:616	arg1	list					618:621	a species-specific glycan mass list	587:621	a species-specific glycan mass list from glycan structure databases and published papers	587:674	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	5	46	theme	isotope	885:891	arg1	distribution					893:904	isotope distribution	885:904	isotope distribution	885:904	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	1	47	theme	glycan	212:217	arg1	lists					224:228	glycan mass lists	212:228	glycan mass lists	212:228	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	5	48	theme	glycan	911:916	arg1	mass					918:921	glycan mass	911:921	glycan mass	911:921	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	3	49	theme	list	618:621	arg1	construction					571:582	the construction	567:582	the construction of a species-specific glycan mass list from glycan structure databases and published papers	567:674	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	1	50	theme	mass	219:222	arg1	lists					224:228	glycan mass lists	212:228	glycan mass lists	212:228	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	5	51	theme	distribution	893:904	arg1	list					923:926	the monoisotope mass, average mass, isotope distribution, and glycan mass list	849:926	the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software	849:957	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	3	52	theme	published	659:667	arg1	papers					669:674	published papers	659:674	published papers	659:674	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	3	53	theme	species-specific	589:604	arg1	list					618:621	a species-specific glycan mass list	587:621	a species-specific glycan mass list from glycan structure databases and published papers	587:674	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	1	54	gly	glycopeptides	278:290	arg2	glycopeptides					278:290	intact glycopeptides	271:290	intact glycopeptides	271:290	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	5	55	theme	mass	918:921	arg1	list					923:926	the monoisotope mass, average mass, isotope distribution, and glycan mass list	849:926	the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software	849:957	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	2	56	theme	events	478:483	arg1	studies					445:451	high throughput studies	429:451	high throughput studies of unusual glycosylation events observed in other species	429:509	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	3	57	theme	glycan	606:611	arg1	list					618:621	a species-specific glycan mass list	587:621	a species-specific glycan mass list from glycan structure databases and published papers	587:674	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	7	58	theme	different	1301:1309	arg1	systems					1330:1336	different complex biological systems	1301:1336	different complex biological systems	1301:1336	Combined, these results show that this strategy is a robust computational approach for the determination of glycan diversity within different complex biological systems.					
34364435	6	59	theme	intact	1134:1139	arg1	glycopeptides					1141:1153	296 and 145 intact glycopeptides	1122:1153	296 and 145 intact glycopeptides respectively	1122:1166	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	7	60	theme	glycan	1277:1282	arg1	diversity					1284:1292	glycan diversity	1277:1292	glycan diversity within different complex biological systems	1277:1336	Combined, these results show that this strategy is a robust computational approach for the determination of glycan diversity within different complex biological systems.					
34364435	3	61	theme	structure	635:643	arg1	databases					645:653	glycan structure databases	628:653	glycan structure databases	628:653	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	1	62	theme	Mass	139:142	arg1	MS					158:159	MS	158:159	MS	158:159	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	1	62	theme	Mass	139:142	arg1	spectrometry					144:155	Mass spectrometry	139:155	Mass spectrometry (MS)-based strategies	139:177	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	3	63	from	construction	571:582	arg1	papers					669:674	published papers	659:674	published papers	659:674	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	3	63	from	construction	571:582	arg1	databases					645:653	glycan structure databases	628:653	glycan structure databases	628:653	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	6	64	gly	glycopeptides	1141:1153	arg2	glycopeptides					1141:1153	296 and 145 intact glycopeptides	1122:1153	296 and 145 intact glycopeptides respectively	1122:1166	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	1	65	theme	spectrometry	144:155	arg1	strategies					168:177	Mass spectrometry (MS)-based strategies	139:177	Mass spectrometry (MS)-based strategies	139:177	Mass spectrometry (MS)-based strategies and related software tools using glycan mass lists have greatly facilitated the analysis of intact glycopeptides.					
34364435	2	66	theme	normal	333:338	arg1	glycans					340:346	normal glycans	333:346	normal glycans of mammals	333:357	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	0	67	theme	glycan	66:71	arg1	library					73:79	a species-specific glycan library	47:79	a species-specific glycan library	47:79	An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses.					
34364435	6	68	theme	O-glycosylation	1035:1049	arg1	N-glycosylation					1001:1015	the N-glycosylation	997:1015	the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978	997:1086	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	6	69	theme	baumannii	1068:1076	arg1	O-glycosylation					1035:1049	O-glycosylation	1035:1049	O-glycosylation of Acinetobacter baumannii ATCC17978	1035:1086	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	6	69	theme	baumannii	1068:1076	arg1	roots					1025:1029	rice roots	1020:1029	rice roots	1020:1029	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	2	70	theme	limited	371:377	arg1	monosaccharides					379:393	limited monosaccharides	371:393	limited monosaccharides	371:393	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34364435	5	71	theme	mass	879:882	arg1	list					923:926	the monoisotope mass, average mass, isotope distribution, and glycan mass list	849:926	the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software	849:957	Then, the software tool generated a glycan library that contained the monoisotope mass, average mass, isotope distribution, and glycan mass list for input into Byonic software.					
34364435	6	72	theme	rice	1020:1023	arg1	roots					1025:1029	rice roots	1020:1029	rice roots	1020:1029	This strategy was applied to analyze the N-glycosylation of rice roots and O-glycosylation of Acinetobacter baumannii ATCC17978, leading to the identification of 296 and 145 intact glycopeptides respectively.					
34364435	0	73	theme	species-specific	49:64	arg1	library					73:79	a species-specific glycan library	47:79	a species-specific glycan library	47:79	An integrated strategy for the construction of a species-specific glycan library for mass spectrometry-based intact glycopeptide analyses.					
34364435	3	74	theme	glycan	628:633	arg1	databases					645:653	glycan structure databases	628:653	glycan structure databases	628:653	In this work, an integrated strategy was developed for the construction of a species-specific glycan mass list from glycan structure databases and published papers.					
34364435	7	75	theme	diversity	1284:1292	arg1	determination					1260:1272	the determination	1256:1272	the determination of glycan diversity within different complex biological systems	1256:1336	Combined, these results show that this strategy is a robust computational approach for the determination of glycan diversity within different complex biological systems.					
34364435	2	76	theme	glycosylation	464:476	arg1	events					478:483	unusual glycosylation events	456:483	unusual glycosylation events observed in other species	456:509	Most glycan mass lists are derived from normal glycans of mammals and contain limited monosaccharides, which has significantly hindered high throughput studies of unusual glycosylation events observed in other species.					
34355537	7	0	theme	asialo	1174:1179	arg1	mAb1					1181:1184	sialylated and asialo mAb1	1159:1184	sialylated and asialo mAb1 provided herein	1159:1200	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
34355537	2	1	theme	structures	329:338	arg1	effects					287:293	the effects	283:293	the effects of highly heterogeneous Fc glycan structures	283:338	However, many of the effects of highly heterogeneous Fc glycan structures have yet to be fully characterized.					
34355537	4	2	from	impact	654:659	arg1	ADCC					696:699	ADCC	696:699	ADCC	696:699	Previous studies on the impact of sialic acid in the Fc glycan on ADCC have not resulted in consistent conclusions.					
34355537	4	2	from	impact	654:659	arg1	glycan					686:691	the Fc glycan	679:691	the Fc glycan on ADCC	679:699	Previous studies on the impact of sialic acid in the Fc glycan on ADCC have not resulted in consistent conclusions.					
34355537	6	3	theme	deamidated	1118:1127	arg1	species					1129:1135	oxidized and deamidated species	1105:1135	oxidized and deamidated species	1105:1135	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	1	4	theme	mAbs	260:263	arg1	safety					225:230	safety	225:230	safety	225:230	It has been well documented that the terminal sugars of Fc glycans can play a critical role in the safety and efficacy of therapeutic mAbs.					
34355537	1	4	theme	mAbs	260:263	arg1	efficacy					236:243	efficacy	236:243	efficacy	236:243	It has been well documented that the terminal sugars of Fc glycans can play a critical role in the safety and efficacy of therapeutic mAbs.					
34355537	6	5	theme	oxidized	1105:1112	arg1	species					1129:1135	oxidized and deamidated species	1105:1135	oxidized and deamidated species	1105:1135	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	4	6	theme	Previous	630:637	arg1	studies					639:645	Previous studies	630:645	Previous studies on the impact of sialic acid in the Fc glycan on ADCC	630:699	Previous studies on the impact of sialic acid in the Fc glycan on ADCC have not resulted in consistent conclusions.					
34355537	3	7	theme	cell	511:514	arg1	surface					516:522	the effector cell surface	498:522	the effector cell surface	498:522	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	1	8	theme	Fc	182:183	arg1	glycans					185:191	Fc glycans	182:191	Fc glycans	182:191	It has been well documented that the terminal sugars of Fc glycans can play a critical role in the safety and efficacy of therapeutic mAbs.					
34355537	2	9	theme	effects	287:293	arg1	many					275:278	many	275:278	many	275:278	However, many of the effects of highly heterogeneous Fc glycan structures have yet to be fully characterized.					
34355537	2	9	theme	effects	287:293	arg1	effects					287:293	the effects	283:293	the effects of highly heterogeneous Fc glycan structures	283:338	However, many of the effects of highly heterogeneous Fc glycan structures have yet to be fully characterized.					
34355537	5	10	theme	kappa	828:832	arg1	chain					840:844	a chimeric murine/human kappa light chain	804:844	a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities	804:913	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	6	11	theme	other	1080:1084	arg1	attributes					1086:1095	other attributes	1080:1095	other attributes such as oxidized and deamidated species	1080:1135	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	6	11	theme	other	1080:1084	arg1	species					1129:1135	oxidized and deamidated species	1105:1135	oxidized and deamidated species	1105:1135	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	7	12	contain	have	1235:1238	arg1	acids					1229:1233	sialic acids	1222:1233	sialic acids	1222:1233	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
34355537	7	12	contain	have	1235:1238	arg2	impact					1253:1258	no impact	1250:1258	no impact on ADCC	1250:1266	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
34355537	7	12	contain	have	1235:1238	arg2	little					1240:1245	little	1240:1245	little	1240:1245	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
34355537	7	13	theme	mAb1	1181:1184	arg1	analysis					1147:1154	The ADCC analysis	1138:1154	The ADCC analysis of sialylated and asialo mAb1 provided herein	1138:1200	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
34355537	5	14	theme	acid-enriched	777:789	arg1	species					791:797	sialic acid-enriched species	770:797	sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities	770:913	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	8	15	theme	therapeutic	1374:1384	arg1	mAbs					1386:1389	therapeutic mAbs	1374:1389	therapeutic mAbs	1374:1389	Altogether, our results highlight the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls.					
34355537	3	16	theme	effector	553:560	arg1	functions					562:570	immune effector functions	546:570	immune effector functions	546:570	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	3	16	theme	effector	553:560	arg1	cytotoxicity					609:620	antibody-dependent cellular cytotoxicity	581:620	antibody-dependent cellular cytotoxicity (ADCC)	581:627	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	3	17	from	receptors	485:493	arg1	surface					516:522	the effector cell surface	498:522	the effector cell surface	498:522	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	4	18	theme	Fc	683:684	arg1	glycan					686:691	the Fc glycan	679:691	the Fc glycan on ADCC	679:699	Previous studies on the impact of sialic acid in the Fc glycan on ADCC have not resulted in consistent conclusions.					
34355537	3	19	theme	cellular	600:607	arg1	ADCC					623:626	ADCC	623:626	ADCC	623:626	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	3	19	theme	cellular	600:607	arg1	cytotoxicity					609:620	antibody-dependent cellular cytotoxicity	581:620	antibody-dependent cellular cytotoxicity (ADCC)	581:627	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	5	20	theme	known	863:867	arg1	binding					887:893	known Fcγ receptor IIIa binding and ADCC activities	863:913	binding	887:893	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	0	21	theme	limited	66:72	arg1	impact					74:79	limited impact	66:79	limited impact	66:79	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	5	22	theme	chain	840:844	arg1	IgG1					846:849	a chimeric murine/human kappa light chain IgG1	804:849	a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities	804:913	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	5	22	theme	chain	840:844	arg1	mAb1					852:855	mAb1	852:855	mAb1	852:855	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	8	23	theme	production	1437:1446	arg1	process					1448:1454	production process	1437:1454	production process controls	1437:1463	Altogether, our results highlight the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls.					
34355537	5	24	with	IgG1	846:849	arg1	binding					887:893	known Fcγ receptor IIIa binding and ADCC activities	863:913	binding	887:893	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	5	24	with	IgG1	846:849	arg1	activities					904:913	known Fcγ receptor IIIa binding and ADCC activities	863:913	activities	904:913	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	4	25	theme	sialic	664:669	arg1	acid					671:674	sialic acid	664:674	sialic acid	664:674	Previous studies on the impact of sialic acid in the Fc glycan on ADCC have not resulted in consistent conclusions.					
34355537	5	26	from	IgG1	846:849	arg1	species					791:797	sialic acid-enriched species	770:797	sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities	770:913	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	0	27	theme	cellular	103:110	arg1	cytotoxicity					112:123	antibody-dependent cellular cytotoxicity	84:123	antibody-dependent cellular cytotoxicity	84:123	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	5	28	theme	ADCC	899:902	arg1	activities					904:913	known Fcγ receptor IIIa binding and ADCC activities	863:913	activities	904:913	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	4	29	theme	consistent	722:731	arg1	conclusions					733:743	consistent conclusions	722:743	consistent conclusions	722:743	Previous studies on the impact of sialic acid in the Fc glycan on ADCC have not resulted in consistent conclusions.					
34355537	0	30	theme	Fc	0:1	arg1	sialylation					10:20	Fc glycan sialylation	0:20	Fc glycan sialylation of biotherapeutic monoclonal antibodies	0:60	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	5	31	theme	receptor	873:880	arg1	binding					887:893	known Fcγ receptor IIIa binding and ADCC activities	863:913	binding	887:893	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	6	32	theme	sialic	978:983	arg1	glycans					1001:1007	sialic acid-containing glycans	978:1007	sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs	978:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	0	33	theme	biotherapeutic	25:38	arg1	antibodies					51:60	biotherapeutic monoclonal antibodies	25:60	biotherapeutic monoclonal antibodies	25:60	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	5	34	theme	murine/human	815:826	arg1	chain					840:844	a chimeric murine/human kappa light chain	804:844	a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities	804:913	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	3	35	theme	Different	376:384	arg1	patterns					400:407	Different glycosylation patterns	376:407	Different glycosylation patterns	376:407	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	8	36	theme	glycan	1331:1336	arg1	strategies					1350:1359	novel glycan engineering strategies	1325:1359	novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls	1325:1463	Altogether, our results highlight the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls.					
34355537	2	37	theme	glycan	322:327	arg1	structures					329:338	highly heterogeneous Fc glycan structures	298:338	highly heterogeneous Fc glycan structures	298:338	However, many of the effects of highly heterogeneous Fc glycan structures have yet to be fully characterized.					
34355537	0	38	theme	antibodies	51:60	arg1	sialylation					10:20	Fc glycan sialylation	0:20	Fc glycan sialylation of biotherapeutic monoclonal antibodies	0:60	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	7	39	theme	sialic	1222:1227	arg1	acids					1229:1233	sialic acids	1222:1233	sialic acids	1222:1233	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
34355537	8	40	theme	strategies	1350:1359	arg1	value					1316:1320	the value	1312:1320	the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls	1312:1463	Altogether, our results highlight the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls.					
34355537	2	41	theme	heterogeneous	305:317	arg1	structures					329:338	highly heterogeneous Fc glycan structures	298:338	highly heterogeneous Fc glycan structures	298:338	However, many of the effects of highly heterogeneous Fc glycan structures have yet to be fully characterized.					
34355537	6	42	theme	therapeutic	1051:1061	arg1	mAbs					1063:1066	therapeutic mAbs	1051:1066	therapeutic mAbs	1051:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	5	43	theme	chimeric	806:813	arg1	chain					840:844	a chimeric murine/human kappa light chain	804:844	a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities	804:913	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	6	44	theme	present	1040:1046	arg1	levels					1033:1038	the typical levels	1021:1038	the typical levels present in therapeutic mAbs	1021:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	6	45	from	present	1040:1046	arg1	mAbs					1063:1066	therapeutic mAbs	1051:1066	therapeutic mAbs	1051:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	3	46	theme	Fcγ	481:483	arg1	receptors					485:493	Fcγ receptors	481:493	Fcγ receptors on the effector cell surface	481:522	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	6	47	theme	typical	1025:1031	arg1	levels					1033:1038	the typical levels	1021:1038	the typical levels present in therapeutic mAbs	1021:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	0	48	contain	has	62:64	arg1	sialylation					10:20	Fc glycan sialylation	0:20	Fc glycan sialylation of biotherapeutic monoclonal antibodies	0:60	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	0	48	contain	has	62:64	arg2	impact					74:79	limited impact	66:79	limited impact	66:79	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	1	49	theme	therapeutic	248:258	arg1	mAbs					260:263	therapeutic mAbs	248:263	therapeutic mAbs	248:263	It has been well documented that the terminal sugars of Fc glycans can play a critical role in the safety and efficacy of therapeutic mAbs.					
34355537	3	50	theme	effector	502:509	arg1	surface					516:522	the effector cell surface	498:522	the effector cell surface	498:522	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	4	51	from	studies	639:645	arg1	impact					654:659	the impact	650:659	the impact of sialic acid in the Fc glycan on ADCC	650:699	Previous studies on the impact of sialic acid in the Fc glycan on ADCC have not resulted in consistent conclusions.					
34355537	6	52	from	increase	966:973	arg1	glycans					1001:1007	sialic acid-containing glycans	978:1007	sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs	978:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	1	53	theme	glycans	185:191	arg1	sugars					172:177	the terminal sugars	159:177	the terminal sugars of Fc glycans	159:191	It has been well documented that the terminal sugars of Fc glycans can play a critical role in the safety and efficacy of therapeutic mAbs.					
34355537	5	54	theme	sialic	770:775	arg1	species					791:797	sialic acid-enriched species	770:797	sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities	770:913	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	1	55	dep	safety	225:230	arg1	the					221:223	the	221:223	the	221:223	It has been well documented that the terminal sugars of Fc glycans can play a critical role in the safety and efficacy of therapeutic mAbs.					
34355537	1	56	theme	critical	204:211	arg1	role					213:216	a critical role	202:216	a critical role	202:216	It has been well documented that the terminal sugars of Fc glycans can play a critical role in the safety and efficacy of therapeutic mAbs.					
34355537	8	57	theme	high-quality	1396:1407	arg1	attributes					1409:1418	high-quality attributes	1396:1418	high-quality attributes	1396:1418	Altogether, our results highlight the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls.					
34355537	3	58	theme	immune	546:551	arg1	functions					562:570	immune effector functions	546:570	immune effector functions	546:570	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	3	58	theme	immune	546:551	arg1	cytotoxicity					609:620	antibody-dependent cellular cytotoxicity	581:620	antibody-dependent cellular cytotoxicity (ADCC)	581:627	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	4	59	theme	acid	671:674	arg1	impact					654:659	the impact	650:659	the impact of sialic acid in the Fc glycan on ADCC	650:699	Previous studies on the impact of sialic acid in the Fc glycan on ADCC have not resulted in consistent conclusions.					
34355537	3	60	theme	antibody-dependent	581:598	arg1	ADCC					623:626	ADCC	623:626	ADCC	623:626	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	3	60	theme	antibody-dependent	581:598	arg1	cytotoxicity					609:620	antibody-dependent cellular cytotoxicity	581:620	antibody-dependent cellular cytotoxicity (ADCC)	581:627	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	8	61	dep	strategies	1350:1359	arg1	designing					1364:1372	designing	1364:1372	designing therapeutic mAbs with high-quality attributes	1364:1418	Altogether, our results highlight the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls.					
34355537	8	61	dep	strategies	1350:1359	arg1	improving					1427:1435	improving	1427:1435	improving production process controls	1427:1463	Altogether, our results highlight the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls.					
34355537	6	62	theme	fourfold	957:964	arg1	increase					966:973	a fourfold increase	955:973	a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs	955:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	7	63	theme	sialylated	1159:1168	arg1	mAb1					1181:1184	sialylated and asialo mAb1	1159:1184	sialylated and asialo mAb1 provided herein	1159:1200	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
34355537	5	64	theme	light	834:838	arg1	chain					840:844	a chimeric murine/human kappa light chain	804:844	a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities	804:913	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	8	65	theme	process	1448:1454	arg1	controls					1456:1463	production process controls	1437:1463	production process controls	1437:1463	Altogether, our results highlight the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls.					
34355537	7	66	theme	ADCC	1142:1145	arg1	analysis					1147:1154	The ADCC analysis	1138:1154	The ADCC analysis of sialylated and asialo mAb1 provided herein	1138:1200	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
34355537	0	67	theme	antibody-dependent	84:101	arg1	cytotoxicity					112:123	antibody-dependent cellular cytotoxicity	84:123	antibody-dependent cellular cytotoxicity	84:123	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	1	68	theme	terminal	163:170	arg1	sugars					172:177	the terminal sugars	159:177	the terminal sugars of Fc glycans	159:191	It has been well documented that the terminal sugars of Fc glycans can play a critical role in the safety and efficacy of therapeutic mAbs.					
34355537	6	69	theme	enriched	922:929	arg1	species					931:937	These enriched species	916:937	These enriched species	916:937	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	0	70	theme	glycan	3:8	arg1	sialylation					10:20	Fc glycan sialylation	0:20	Fc glycan sialylation of biotherapeutic monoclonal antibodies	0:60	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	4	71	from	ADCC	696:699	arg1	impact					654:659	the impact	650:659	the impact of sialic acid in the Fc glycan on ADCC	650:699	Previous studies on the impact of sialic acid in the Fc glycan on ADCC have not resulted in consistent conclusions.					
34355537	5	72	theme	Fcγ	869:871	arg1	binding					887:893	known Fcγ receptor IIIa binding and ADCC activities	863:913	binding	887:893	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	6	73	theme	relative	1009:1016	arg1	glycans					1001:1007	sialic acid-containing glycans	978:1007	sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs	978:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	7	74	gly	sialylated	1159:1168	arg1	mAb1					1181:1184	sialylated and asialo mAb1	1159:1184	sialylated and asialo mAb1 provided herein	1159:1200	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
34355537	3	75	theme	Fc-dependent	420:431	arg1	activities					433:442	Fc-dependent activities	420:442	Fc-dependent activities	420:442	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	3	75	theme	Fc-dependent	420:431	arg1	ability					457:463	the ability	453:463	the ability of mAbs to bind Fcγ receptors on the effector cell surface	453:522	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	4	76	from	glycan	686:691	arg1	ADCC					696:699	ADCC	696:699	ADCC	696:699	Previous studies on the impact of sialic acid in the Fc glycan on ADCC have not resulted in consistent conclusions.					
34355537	5	77	theme	IIIa	882:885	arg1	binding					887:893	known Fcγ receptor IIIa binding and ADCC activities	863:913	binding	887:893	In our study, we tested sialic acid-enriched species from a chimeric murine/human kappa light chain IgG1 (mAb1) with known Fcγ receptor IIIa binding and ADCC activities.					
34355537	6	78	theme	acid-containing	985:999	arg1	glycans					1001:1007	sialic acid-containing glycans	978:1007	sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs	978:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	6	79	attach	present	1040:1046	arg1	mAbs					1063:1066	therapeutic mAbs	1051:1066	therapeutic mAbs	1051:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	6	79	attach	present	1040:1046	arg2	levels					1033:1038	the typical levels	1021:1038	the typical levels present in therapeutic mAbs	1021:1066	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	0	80	theme	monoclonal	40:49	arg1	antibodies					51:60	biotherapeutic monoclonal antibodies	25:60	biotherapeutic monoclonal antibodies	25:60	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	6	81	from	mAbs	1063:1066	arg1	present					1040:1046	present	1040:1046	present	1040:1046	These enriched species contained up to a fourfold increase in sialic acid-containing glycans relative to the typical levels present in therapeutic mAbs, along with other attributes such as oxidized and deamidated species.					
34355537	0	82	gly	sialylation	10:20	arg1	antibodies					51:60	biotherapeutic monoclonal antibodies	25:60	biotherapeutic monoclonal antibodies	25:60	Fc glycan sialylation of biotherapeutic monoclonal antibodies has limited impact on antibody-dependent cellular cytotoxicity.					
34355537	3	83	theme	glycosylation	386:398	arg1	patterns					400:407	Different glycosylation patterns	376:407	Different glycosylation patterns	376:407	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	8	84	theme	novel	1325:1329	arg1	strategies					1350:1359	novel glycan engineering strategies	1325:1359	novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls	1325:1463	Altogether, our results highlight the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls.					
34355537	2	85	theme	Fc	319:320	arg1	structures					329:338	highly heterogeneous Fc glycan structures	298:338	highly heterogeneous Fc glycan structures	298:338	However, many of the effects of highly heterogeneous Fc glycan structures have yet to be fully characterized.					
34355537	8	86	theme	engineering	1338:1348	arg1	strategies					1350:1359	novel glycan engineering strategies	1325:1359	novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls	1325:1463	Altogether, our results highlight the value of novel glycan engineering strategies in designing therapeutic mAbs with high-quality attributes and in improving production process controls.					
34355537	3	87	theme	mAbs	468:471	arg1	ability					457:463	the ability	453:463	the ability of mAbs to bind Fcγ receptors on the effector cell surface	453:522	Different glycosylation patterns can affect Fc-dependent activities, such as the ability of mAbs to bind Fcγ receptors on the effector cell surface, which is critical to immune effector functions, such as antibody-dependent cellular cytotoxicity (ADCC).					
34355537	7	88	dep	little	1240:1245	arg1	activity					1268:1275	activity	1268:1275	activity	1268:1275	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
34355537	7	89	from	impact	1253:1258	arg1	ADCC					1263:1266	ADCC	1263:1266	ADCC	1263:1266	The ADCC analysis of sialylated and asialo mAb1 provided herein shows evidence that sialic acids have little or no impact on ADCC activity.					
33881228	6	0	theme	mannose-enriched	1012:1027	arg1	glycans					1029:1035	unusual mannose-enriched glycans	1004:1035	unusual mannose-enriched glycans that are typically found in lower microorganisms	1004:1084	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	4	1	theme	systemic	644:651	arg1	erythematosus					659:671	systemic lupus erythematosus	644:671	systemic lupus erythematosus	644:671	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	5	2	theme	mass	728:731	arg1	spectrometry					733:744	advanced tissue mass spectrometry	712:744	advanced tissue mass spectrometry	712:744	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	8	3	theme	cell	1242:1245	arg1	surface					1247:1253	the cell surface	1238:1253	the cell surface of kidney cells	1238:1269	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	10	4	theme	chronic	1676:1682	arg1	CKD					1700:1702	CKD	1700:1702	CKD	1700:1702	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	10	4	theme	chronic	1676:1682	arg1	disease					1691:1697	chronic kidney disease	1676:1697	chronic kidney disease (CKD)	1676:1703	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	6	5	theme	spatial	980:986	arg1	distribution					988:999	spatial distribution	980:999	spatial distribution	980:999	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	10	6	theme	mannosylation	1526:1538	arg1	levels					1540:1545	mannosylation levels	1526:1545	mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis	1526:1626	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	2	7	theme	response	282:289	arg1	molecules					309:317	important molecules	299:317	important molecules in self-nonself discrimination	299:348	Glycans are master regulators of the inflammatory response and are important molecules in self-nonself discrimination.					
33881228	2	7	theme	response	282:289	arg1	regulators					251:260	master regulators	244:260	master regulators of the inflammatory response	244:289	Glycans are master regulators of the inflammatory response and are important molecules in self-nonself discrimination.					
33881228	2	7	theme	response	282:289	arg1	Glycans					232:238	Glycans	232:238	Glycans	232:238	Glycans are master regulators of the inflammatory response and are important molecules in self-nonself discrimination.					
33881228	8	8	theme	cells	1265:1269	arg1	surface					1247:1253	the cell surface	1238:1253	the cell surface of kidney cells	1238:1269	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	5	9	theme	advanced	712:719	arg1	spectrometry					733:744	advanced tissue mass spectrometry	712:744	advanced tissue mass spectrometry	712:744	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	2	10	theme	self-nonself	322:333	arg1	discrimination					335:348	self-nonself discrimination	322:348	self-nonself discrimination	322:348	Glycans are master regulators of the inflammatory response and are important molecules in self-nonself discrimination.					
33881228	10	11	theme	disease	1691:1697	arg1	development					1661:1671	the development	1657:1671	the development of chronic kidney disease (CKD)	1657:1703	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	5	12	theme	ex	783:784	arg1	glycophenotyping					791:806	ex vivo glycophenotyping	783:806	ex vivo glycophenotyping	783:806	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	10	13	theme	%	1712:1712	arg1	specificity					1714:1724	93% specificity	1710:1724	93% specificity	1710:1724	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	5	14	theme	glycophenotyping	791:806	arg1	combination					697:707	A combination	695:707	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping	695:806	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	1	15	theme	diseases	222:229	arg1	Changes					155:161	OBJECTIVE Changes	145:161	OBJECTIVE Changes in protein glycosylation	145:186	OBJECTIVE Changes in protein glycosylation are a hallmark of immune-mediated diseases.					
33881228	1	15	theme	diseases	222:229	arg1	hallmark					194:201	a hallmark	192:201	a hallmark of immune-mediated diseases	192:229	OBJECTIVE Changes in protein glycosylation are a hallmark of immune-mediated diseases.					
33881228	8	16	theme	specific	1307:1314	arg1	receptors					1335:1343	specific glycan-recognizing receptors	1307:1343	specific glycan-recognizing receptors expressed by immune cells	1307:1369	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	7	17	theme	other	1154:1158	arg1	diseases					1167:1174	other kidney diseases	1154:1174	other kidney diseases	1154:1174	This glycosignature was specific for LN, as it was not observed in other kidney diseases.					
33881228	5	18	from	repertoire	846:855	arg1	samples					883:889	LN tissue samples	873:889	LN tissue samples	873:889	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	4	19	with	patients	630:637	arg1	erythematosus					659:671	systemic lupus erythematosus	644:671	systemic lupus erythematosus	644:671	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	4	19	with	patients	630:637	arg1	LN					691:692	biopsy-proven LN	677:692	biopsy-proven LN	677:692	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	9	20	theme	abnormal	1377:1384	arg1	glycosignature					1386:1399	This abnormal glycosignature	1372:1399	This abnormal glycosignature of LN	1372:1405	This abnormal glycosignature of LN was shown to be due to a deficient complex N-glycosylation pathway and a proficient O-mannosylation pathway.					
33881228	1	21	theme	OBJECTIVE	145:153	arg1	Changes					155:161	OBJECTIVE Changes	145:161	OBJECTIVE Changes in protein glycosylation	145:186	OBJECTIVE Changes in protein glycosylation are a hallmark of immune-mediated diseases.					
33881228	1	21	theme	OBJECTIVE	145:153	arg1	hallmark					194:201	a hallmark	192:201	a hallmark of immune-mediated diseases	192:229	OBJECTIVE Changes in protein glycosylation are a hallmark of immune-mediated diseases.					
33881228	10	22	theme	kidney	1559:1564	arg1	samples					1573:1579	kidney biopsy samples	1559:1579	kidney biopsy samples from patients with LN at the time of diagnosis	1559:1626	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	0	23	theme	Nephritis	72:80	arg1	Biomarker					53:61	a Diagnostic and Prognostic Biomarker	25:61	a Diagnostic and Prognostic Biomarker of Lupus Nephritis	25:80	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	1	24	from	Changes	155:161	arg1	glycosylation					174:186	protein glycosylation	166:186	protein glycosylation	166:186	OBJECTIVE Changes in protein glycosylation are a hallmark of immune-mediated diseases.					
33881228	10	25	from	time	1610:1613	arg1	LN					1600:1601	LN	1600:1601	LN at the time of diagnosis	1600:1626	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	4	26	theme	tissue	557:562	arg1	characterization					574:589	A comprehensive tissue glycomics characterization	541:589	A comprehensive tissue glycomics characterization	541:589	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	11	27	theme	potential	1839:1847	arg1	glycobiomarker					1849:1862	a potential glycobiomarker	1837:1862	a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN	1837:1926	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	5	28	dep	in	747:748	arg1	situ					750:753	situ	750:753	situ	750:753	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	0	29	theme	Glycan	94:99	arg1	Neoepitope					101:110	An Unusual Glycan Neoepitope	83:110	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.	0:143	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	8	30	gly	mannosylated	1189:1200	arg1	glycans					1202:1208	mannosylated glycans	1189:1208	mannosylated glycans	1189:1208	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	0	31	theme	Protein	0:6	arg1	Mannosylation					8:20	Protein Mannosylation	0:20	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.	0:143	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	4	32	dep	METHODS	533:539	arg1	performed					595:603	performed	595:603	was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN	591:692	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	3	33	theme	lupus	400:404	arg1	LN					417:418	LN	417:418	LN	417:418	This study was undertaken to investigate whether lupus nephritis (LN) exhibits altered cellular glycosylation to identify a unique glycosignature that characterizes LN pathogenesis.					
33881228	3	33	theme	lupus	400:404	arg1	nephritis					406:414	lupus nephritis	400:414	lupus nephritis (LN)	400:419	This study was undertaken to investigate whether lupus nephritis (LN) exhibits altered cellular glycosylation to identify a unique glycosignature that characterizes LN pathogenesis.					
33881228	4	34	theme	kidney	608:613	arg1	specimens					615:623	kidney specimens	608:623	kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN	608:692	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	6	35	theme	increased	956:964	arg1	abundance					966:974	increased abundance	956:974	increased abundance	956:974	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	10	36	with	patients	1586:1593	arg1	LN					1600:1601	LN	1600:1601	LN at the time of diagnosis	1600:1626	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	3	37	theme	altered	430:436	arg1	glycosylation					447:459	altered cellular glycosylation	430:459	altered cellular glycosylation	430:459	This study was undertaken to investigate whether lupus nephritis (LN) exhibits altered cellular glycosylation to identify a unique glycosignature that characterizes LN pathogenesis.					
33881228	3	38	theme	unique	475:480	arg1	glycosignature					482:495	a unique glycosignature	473:495	a unique glycosignature that characterizes LN pathogenesis	473:530	This study was undertaken to investigate whether lupus nephritis (LN) exhibits altered cellular glycosylation to identify a unique glycosignature that characterizes LN pathogenesis.					
33881228	0	39	theme	Diagnostic	27:36	arg1	Biomarker					53:61	a Diagnostic and Prognostic Biomarker	25:61	a Diagnostic and Prognostic Biomarker of Lupus Nephritis	25:80	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	9	40	theme	complex	1442:1448	arg1	pathway					1466:1472	a deficient complex N-glycosylation pathway	1430:1472	a deficient complex N-glycosylation pathway	1430:1472	This abnormal glycosignature of LN was shown to be due to a deficient complex N-glycosylation pathway and a proficient O-mannosylation pathway.					
33881228	5	41	theme	tissue	876:881	arg1	samples					883:889	LN tissue samples	873:889	LN tissue samples	873:889	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	11	42	dep	marker	1766:1771	arg1	representing					1824:1835	representing	1824:1835	thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN	1819:1926	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	11	42	dep	marker	1766:1771	arg1	predicting					1780:1789	predicting	1780:1789	predicting the development of CKD	1780:1812	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	8	43	theme	glycans	1202:1208	arg1	Exposure					1177:1184	Exposure	1177:1184	Exposure of mannosylated glycans in LN	1177:1214	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	6	44	theme	glycan	922:927	arg1	signature					929:937	a unique glycan signature	913:937	a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms	913:1084	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	3	45	theme	LN	516:517	arg1	pathogenesis					519:530	LN pathogenesis	516:530	LN pathogenesis	516:530	This study was undertaken to investigate whether lupus nephritis (LN) exhibits altered cellular glycosylation to identify a unique glycosignature that characterizes LN pathogenesis.					
33881228	9	46	theme	O-mannosylation	1491:1505	arg1	pathway					1507:1513	a proficient O-mannosylation pathway	1478:1513	a proficient O-mannosylation pathway	1478:1513	This abnormal glycosignature of LN was shown to be due to a deficient complex N-glycosylation pathway and a proficient O-mannosylation pathway.					
33881228	6	47	theme	glycans	1029:1035	arg1	abundance					966:974	increased abundance	956:974	increased abundance	956:974	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	6	47	theme	glycans	1029:1035	arg1	distribution					988:999	spatial distribution	980:999	spatial distribution	980:999	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	5	48	theme	tissue	721:726	arg1	spectrometry					733:744	advanced tissue mass spectrometry	712:744	advanced tissue mass spectrometry	712:744	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	6	49	theme	unusual	1004:1010	arg1	glycans					1029:1035	unusual mannose-enriched glycans	1004:1035	unusual mannose-enriched glycans that are typically found in lower microorganisms	1004:1084	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	10	50	theme	kidney	1684:1689	arg1	CKD					1700:1702	CKD	1700:1702	CKD	1700:1702	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	10	50	theme	kidney	1684:1689	arg1	disease					1691:1697	chronic kidney disease	1676:1697	chronic kidney disease (CKD)	1676:1703	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	11	51	theme	CONCLUSION	1727:1736	arg1	mannosylation					1747:1759	CONCLUSION Cellular mannosylation	1727:1759	CONCLUSION Cellular mannosylation	1727:1759	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	11	51	theme	CONCLUSION	1727:1736	arg1	marker					1766:1771	a marker	1764:1771	a marker	1764:1771	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	5	52	theme	spectrometry	733:744	arg1	combination					697:707	A combination	695:707	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping	695:806	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	6	53	theme	RESULTS	892:898	arg1	LN					900:901	RESULTS LN	892:901	RESULTS LN	892:901	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	2	54	theme	important	299:307	arg1	molecules					309:317	important molecules	299:317	important molecules in self-nonself discrimination	299:348	Glycans are master regulators of the inflammatory response and are important molecules in self-nonself discrimination.					
33881228	4	55	theme	lupus	653:657	arg1	erythematosus					659:671	systemic lupus erythematosus	644:671	systemic lupus erythematosus	644:671	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	1	56	theme	protein	166:172	arg1	glycosylation					174:186	protein glycosylation	166:186	protein glycosylation	166:186	OBJECTIVE Changes in protein glycosylation are a hallmark of immune-mediated diseases.					
33881228	0	57	theme	Prognostic	42:51	arg1	Biomarker					53:61	a Diagnostic and Prognostic Biomarker	25:61	a Diagnostic and Prognostic Biomarker of Lupus Nephritis	25:80	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	8	58	theme	glycan-recognizing	1316:1333	arg1	receptors					1335:1343	specific glycan-recognizing receptors	1307:1343	specific glycan-recognizing receptors expressed by immune cells	1307:1369	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	8	59	theme	kidney	1258:1263	arg1	cells					1265:1269	kidney cells	1258:1269	kidney cells	1258:1269	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	11	60	theme	diagnostic	1886:1895	arg1	algorithm					1912:1920	the diagnostic and prognostic algorithm	1882:1920	the diagnostic and prognostic algorithm of LN	1882:1926	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	7	61	theme	kidney	1160:1165	arg1	diseases					1167:1174	other kidney diseases	1154:1174	other kidney diseases	1154:1174	This glycosignature was specific for LN, as it was not observed in other kidney diseases.					
33881228	6	62	theme	lower	1065:1069	arg1	microorganisms					1071:1084	lower microorganisms	1065:1084	lower microorganisms	1065:1084	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	8	63	theme	immune	1358:1363	arg1	cells					1365:1369	immune cells	1358:1369	immune cells	1358:1369	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	2	64	theme	master	244:249	arg1	Glycans					232:238	Glycans	232:238	Glycans	232:238	Glycans are master regulators of the inflammatory response and are important molecules in self-nonself discrimination.					
33881228	2	64	theme	master	244:249	arg1	regulators					251:260	master regulators	244:260	master regulators of the inflammatory response	244:289	Glycans are master regulators of the inflammatory response and are important molecules in self-nonself discrimination.					
33881228	1	65	theme	immune-mediated	206:220	arg1	diseases					222:229	immune-mediated diseases	206:229	immune-mediated diseases	206:229	OBJECTIVE Changes in protein glycosylation are a hallmark of immune-mediated diseases.					
33881228	8	66	theme	increased	1282:1290	arg1	recognition					1292:1302	increased recognition	1282:1302	increased recognition by specific glycan-recognizing receptors expressed by immune cells	1282:1369	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	10	67	located	detected	1547:1554	arg1	samples					1573:1579	kidney biopsy samples	1559:1579	kidney biopsy samples from patients with LN at the time of diagnosis	1559:1626	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	10	67	located	detected	1547:1554	arg2	levels					1540:1545	mannosylation levels	1526:1545	mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis	1526:1626	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	10	68	theme	93	1710:1711	arg1	%					1712:1712	%	1712:1712	%	1712:1712	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	5	69	theme	in	747:748	arg1	glyco-characterization					755:776	in situ glyco-characterization	747:776	in situ glyco-characterization	747:776	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	10	70	from	patients	1586:1593	arg1	samples					1573:1579	kidney biopsy samples	1559:1579	kidney biopsy samples from patients with LN at the time of diagnosis	1559:1626	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	0	71	theme	Systemic	115:122	arg1	Erythematosus					130:142	Systemic Lupus Erythematosus	115:142	Systemic Lupus Erythematosus	115:142	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	0	72	from	Neoepitope	101:110	arg1	Erythematosus					130:142	Systemic Lupus Erythematosus	115:142	Systemic Lupus Erythematosus	115:142	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	11	73	theme	prognostic	1901:1910	arg1	algorithm					1912:1920	the diagnostic and prognostic algorithm	1882:1920	the diagnostic and prognostic algorithm of LN	1882:1926	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	5	74	theme	glyco-characterization	755:776	arg1	combination					697:707	A combination	695:707	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping	695:806	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	2	75	theme	inflammatory	269:280	arg1	response					282:289	the inflammatory response	265:289	the inflammatory response	265:289	Glycans are master regulators of the inflammatory response and are important molecules in self-nonself discrimination.					
33881228	11	76	theme	LN	1925:1926	arg1	algorithm					1912:1920	the diagnostic and prognostic algorithm	1882:1920	the diagnostic and prognostic algorithm of LN	1882:1926	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	0	77	theme	Lupus	66:70	arg1	Nephritis					72:80	Lupus Nephritis	66:80	Lupus Nephritis	66:80	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	8	78	from	Exposure	1177:1184	arg1	LN					1213:1214	LN	1213:1214	LN	1213:1214	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	4	79	theme	comprehensive	543:555	arg1	characterization					574:589	A comprehensive tissue glycomics characterization	541:589	A comprehensive tissue glycomics characterization	541:589	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	2	80	from	molecules	309:317	arg1	discrimination					335:348	self-nonself discrimination	322:348	self-nonself discrimination	322:348	Glycans are master regulators of the inflammatory response and are important molecules in self-nonself discrimination.					
33881228	5	81	dep	ex	783:784	arg1	vivo					786:789	vivo	786:789	vivo	786:789	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	4	82	theme	glycomics	564:572	arg1	characterization					574:589	A comprehensive tissue glycomics characterization	541:589	A comprehensive tissue glycomics characterization	541:589	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	0	83	theme	Unusual	86:92	arg1	Neoepitope					101:110	An Unusual Glycan Neoepitope	83:110	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.	0:143	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	9	84	theme	LN	1404:1405	arg1	glycosignature					1386:1399	This abnormal glycosignature	1372:1399	This abnormal glycosignature of LN	1372:1405	This abnormal glycosignature of LN was shown to be due to a deficient complex N-glycosylation pathway and a proficient O-mannosylation pathway.					
33881228	7	85	located	observed	1142:1149	arg2	it					1131:1132	it	1131:1132	it	1131:1132	This glycosignature was specific for LN, as it was not observed in other kidney diseases.					
33881228	7	85	located	observed	1142:1149	arg1	diseases					1167:1174	other kidney diseases	1154:1174	other kidney diseases	1154:1174	This glycosignature was specific for LN, as it was not observed in other kidney diseases.					
33881228	4	86	from	patients	630:637	arg1	specimens					615:623	kidney specimens	608:623	kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN	608:692	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	10	87	theme	biopsy	1566:1571	arg1	samples					1573:1579	kidney biopsy samples	1559:1579	kidney biopsy samples from patients with LN at the time of diagnosis	1559:1626	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	2	88	from	regulators	251:260	arg1	discrimination					335:348	self-nonself discrimination	322:348	self-nonself discrimination	322:348	Glycans are master regulators of the inflammatory response and are important molecules in self-nonself discrimination.					
33881228	5	89	theme	N-glycans	860:868	arg1	N-glycans					860:868	N-glycans	860:868	N-glycans	860:868	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	5	89	theme	N-glycans	860:868	arg1	repertoire					846:855	the repertoire	842:855	the repertoire of N-glycans in LN tissue samples	842:889	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	9	90	theme	deficient	1432:1440	arg1	pathway					1466:1472	a deficient complex N-glycosylation pathway	1430:1472	a deficient complex N-glycosylation pathway	1430:1472	This abnormal glycosignature of LN was shown to be due to a deficient complex N-glycosylation pathway and a proficient O-mannosylation pathway.					
33881228	8	91	theme	mannosylated	1189:1200	arg1	glycans					1202:1208	mannosylated glycans	1189:1208	mannosylated glycans	1189:1208	Exposure of mannosylated glycans in LN was shown to occur at the cell surface of kidney cells, promoting increased recognition by specific glycan-recognizing receptors expressed by immune cells.					
33881228	3	92	theme	cellular	438:445	arg1	glycosylation					447:459	altered cellular glycosylation	430:459	altered cellular glycosylation	430:459	This study was undertaken to investigate whether lupus nephritis (LN) exhibits altered cellular glycosylation to identify a unique glycosignature that characterizes LN pathogenesis.					
33881228	11	93	theme	LN	1776:1777	arg1	mannosylation					1747:1759	CONCLUSION Cellular mannosylation	1727:1759	CONCLUSION Cellular mannosylation	1727:1759	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	11	93	theme	LN	1776:1777	arg1	marker					1766:1771	a marker	1764:1771	a marker	1764:1771	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	6	94	located	found	1056:1060	arg2	glycans					1029:1035	unusual mannose-enriched glycans	1004:1035	unusual mannose-enriched glycans that are typically found in lower microorganisms	1004:1084	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	6	94	located	found	1056:1060	arg1	microorganisms					1071:1084	lower microorganisms	1065:1084	lower microorganisms	1065:1084	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	5	95	theme	LN	873:874	arg1	samples					883:889	LN tissue samples	873:889	LN tissue samples	873:889	A combination of advanced tissue mass spectrometry, in situ glyco-characterization, and ex vivo glycophenotyping was performed to structurally map the repertoire of N-glycans in LN tissue samples.					
33881228	9	96	theme	N-glycosylation	1450:1464	arg1	pathway					1466:1472	a deficient complex N-glycosylation pathway	1430:1472	a deficient complex N-glycosylation pathway	1430:1472	This abnormal glycosignature of LN was shown to be due to a deficient complex N-glycosylation pathway and a proficient O-mannosylation pathway.					
33881228	0	97	theme	Lupus	124:128	arg1	Erythematosus					130:142	Systemic Lupus Erythematosus	115:142	Systemic Lupus Erythematosus	115:142	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	10	98	theme	diagnosis	1618:1626	arg1	time					1610:1613	the time	1606:1613	the time of diagnosis	1606:1626	Moreover, mannosylation levels detected in kidney biopsy samples from patients with LN at the time of diagnosis were demonstrated to predict the development of chronic kidney disease (CKD) with 93% specificity.					
33881228	11	99	theme	CKD	1810:1812	arg1	development					1795:1805	the development	1791:1805	the development of CKD	1791:1812	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	6	100	theme	unique	915:920	arg1	signature					929:937	a unique glycan signature	913:937	a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms	913:1084	RESULTS LN exhibited a unique glycan signature characterized by increased abundance and spatial distribution of unusual mannose-enriched glycans that are typically found in lower microorganisms.					
33881228	4	101	theme	biopsy-proven	677:689	arg1	LN					691:692	biopsy-proven LN	677:692	biopsy-proven LN	677:692	METHODS A comprehensive tissue glycomics characterization was performed in kidney specimens from patients with systemic lupus erythematosus and biopsy-proven LN.					
33881228	9	102	theme	proficient	1480:1489	arg1	pathway					1507:1513	a proficient O-mannosylation pathway	1478:1513	a proficient O-mannosylation pathway	1478:1513	This abnormal glycosignature of LN was shown to be due to a deficient complex N-glycosylation pathway and a proficient O-mannosylation pathway.					
33881228	0	103	dep	Mannosylation	8:20	arg1	Neoepitope					101:110	An Unusual Glycan Neoepitope	83:110	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.	0:143	Protein Mannosylation as a Diagnostic and Prognostic Biomarker of Lupus Nephritis: An Unusual Glycan Neoepitope in Systemic Lupus Erythematosus.					
33881228	11	104	theme	Cellular	1738:1745	arg1	mannosylation					1747:1759	CONCLUSION Cellular mannosylation	1727:1759	CONCLUSION Cellular mannosylation	1727:1759	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33881228	11	104	theme	Cellular	1738:1745	arg1	marker					1766:1771	a marker	1764:1771	a marker	1764:1771	CONCLUSION Cellular mannosylation is a marker of LN, predicting the development of CKD, and thus representing a potential glycobiomarker to be included in the diagnostic and prognostic algorithm of LN.					
33591717	9	0	theme	synthetic	1664:1672	arg1	molecules					1678:1686	synthetic WTA molecules	1664:1686	synthetic WTA molecules	1664:1686	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	9	0	theme	synthetic	1664:1672	arg1	tool					1712:1715	a novel glycobiology tool	1691:1715	a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis	1691:1798	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	6	1	theme	linkage	1054:1060	arg1	position					1062:1069	β-GlcNAc linkage position	1045:1069	β-GlcNAc linkage position	1045:1069	Here, we aimed to unravel the impact of β-GlcNAc linkage position for langerin binding and LC activation.					
33591717	10	2	dep	S.	1881:1882	arg1	aureus					1884:1889	aureus	1884:1889	aureus	1884:1889	Overall, our data suggest that LCs are able to sense all β-GlcNAc-WTA producing S. aureus strains, likely performing an important role as first responders upon S. aureus skin invasion.					
33591717	4	3	from	TarS	727:730	arg1	wall					768:771	the cell wall glycopolymer wall teichoic acid (WTA)	759:809	the cell wall glycopolymer wall teichoic acid (WTA)	759:809	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	1	4	theme	skin	171:174	arg1	aureus					140:145	Staphylococcus aureus	125:145	Staphylococcus aureus	125:145	Staphylococcus aureus is the leading cause of skin and soft tissue infections.					
33591717	1	4	theme	skin	171:174	arg1	cause					162:166	the leading cause	150:166	the leading cause of skin and soft tissue infections	150:201	Staphylococcus aureus is the leading cause of skin and soft tissue infections.					
33591717	9	5	theme	glycobiology	1699:1710	arg1	molecules					1678:1686	synthetic WTA molecules	1664:1686	synthetic WTA molecules	1664:1686	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	9	5	theme	glycobiology	1699:1710	arg1	tool					1712:1715	a novel glycobiology tool	1691:1715	a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis	1691:1798	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	4	6	theme	dedicated	696:704	arg1	TarS					727:730	dedicated glycosyltransferases TarS	696:730	dedicated glycosyltransferases TarS	696:730	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	1	7	theme	soft	180:183	arg1	infections					192:201	soft tissue infections	180:201	soft tissue infections	180:201	Staphylococcus aureus is the leading cause of skin and soft tissue infections.					
33591717	1	8	theme	infections	192:201	arg1	aureus					140:145	Staphylococcus aureus	125:145	Staphylococcus aureus	125:145	Staphylococcus aureus is the leading cause of skin and soft tissue infections.					
33591717	1	8	theme	infections	192:201	arg1	cause					162:166	the leading cause	150:166	the leading cause of skin and soft tissue infections	150:201	Staphylococcus aureus is the leading cause of skin and soft tissue infections.					
33591717	5	9	theme	alternative	825:835	arg1	TarP					862:865	TarP	862:865	TarP	862:865	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	5	9	theme	alternative	825:835	arg1	glycosyltransferase					841:859	an alternative WTA glycosyltransferase	822:859	an alternative WTA glycosyltransferase	822:859	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	7	10	theme	vitro-generated	1350:1364	arg1	LCs					1366:1368	in vitro-generated LCs	1347:1368	in vitro-generated LCs	1347:1368	Using genetically modified S. aureus strains, we observed that langerin similarly recognized bacteria that produce either TarS- or TarP-modified WTA, yet tarP-expressing S. aureus induced increased cytokine production and maturation of in vitro-generated LCs compared to tarS-expressing S. aureus.					
33591717	4	11	dep	wall	768:771	arg1	WTA					806:808	WTA	806:808	WTA	806:808	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	4	11	dep	wall	768:771	arg1	acid					800:803	glycopolymer wall teichoic acid	773:803	the cell wall glycopolymer wall teichoic acid (WTA)	759:809	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	9	12	theme	S.	1767:1768	arg1	pathogenesis					1787:1798	S. aureus molecular pathogenesis	1767:1798	S. aureus molecular pathogenesis	1767:1798	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	8	13	theme	different	1469:1477	arg1	patterns					1507:1514	the different S. aureus WTA glycosylation patterns	1465:1514	the different S. aureus WTA glycosylation patterns	1465:1514	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	9	14	theme	molecular	1777:1785	arg1	pathogenesis					1787:1798	S. aureus molecular pathogenesis	1767:1798	S. aureus molecular pathogenesis	1767:1798	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	0	15	theme	Teichoic	55:62	arg1	Acid					64:67	Staphylococcus aureus Wall Teichoic Acid	28:67	Staphylococcus aureus Wall Teichoic Acid	28:67	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	5	16	theme	WTA	837:839	arg1	TarP					862:865	TarP	862:865	TarP	862:865	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	5	16	theme	WTA	837:839	arg1	glycosyltransferase					841:859	an alternative WTA glycosyltransferase	822:859	an alternative WTA glycosyltransferase	822:859	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	8	17	theme	glycosylation	1493:1505	arg1	patterns					1507:1514	the different S. aureus WTA glycosylation patterns	1465:1514	the different S. aureus WTA glycosylation patterns	1465:1514	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	0	18	from	Impact	0:5	arg1	Activation					113:122	Langerhans Cell Activation	97:122	Langerhans Cell Activation	97:122	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	0	18	from	Impact	0:5	arg1	Recognition					81:91	Langerin Recognition	72:91	Langerin Recognition	72:91	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	4	19	theme	teichoic	791:798	arg1	WTA					806:808	WTA	806:808	WTA	806:808	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	4	19	theme	teichoic	791:798	arg1	acid					800:803	glycopolymer wall teichoic acid	773:803	the cell wall glycopolymer wall teichoic acid (WTA)	759:809	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	8	20	used	used	1522:1525	arg2	molecules					1436:1444	Chemically synthesized WTA molecules	1409:1444	Chemically synthesized WTA molecules	1409:1444	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	8	20	used	used	1522:1525	arg2	representative					1447:1460	representative	1447:1460	representative of the different S. aureus WTA glycosylation patterns	1447:1514	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	3	21	theme	Langerhans	348:357	arg1	cells					359:363	Langerhans cells	348:363	Langerhans cells (LCs)	348:369	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	3	21	theme	Langerhans	348:357	arg1	type					418:421	the only professional antigen-presenting cell type	372:421	the only professional antigen-presenting cell type in the epidermis	372:438	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	3	21	theme	Langerhans	348:357	arg1	LCs					366:368	LCs	366:368	LCs	366:368	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	0	22	theme	Langerhans	97:106	arg1	Activation					113:122	Langerhans Cell Activation	97:122	Langerhans Cell Activation	97:122	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	5	23	theme	WTA	967:969	arg1	subunit					996:1002	the WTA ribitol phosphate (RboP) subunit	963:1002	the WTA ribitol phosphate (RboP) subunit	963:1002	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	4	24	theme	cell	763:766	arg1	wall					768:771	the cell wall glycopolymer wall teichoic acid (WTA)	759:809	the cell wall glycopolymer wall teichoic acid (WTA)	759:809	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	7	25	theme	increased	1299:1307	arg1	production					1318:1327	cytokine production	1309:1327	cytokine production	1309:1327	Using genetically modified S. aureus strains, we observed that langerin similarly recognized bacteria that produce either TarS- or TarP-modified WTA, yet tarP-expressing S. aureus induced increased cytokine production and maturation of in vitro-generated LCs compared to tarS-expressing S. aureus.					
33591717	8	26	theme	binding	1552:1558	arg1	requirements					1560:1571	langerin-WTA binding requirements	1539:1571	langerin-WTA binding requirements	1539:1571	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	4	27	theme	glycopolymer	773:784	arg1	WTA					806:808	WTA	806:808	WTA	806:808	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	4	27	theme	glycopolymer	773:784	arg1	acid					800:803	glycopolymer wall teichoic acid	773:803	the cell wall glycopolymer wall teichoic acid (WTA)	759:809	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	0	28	theme	Glycan	10:15	arg1	Linkage					17:23	Glycan Linkage	10:23	Glycan Linkage	10:23	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	8	29	dep	S.	1479:1480	arg1	aureus					1482:1487	aureus	1482:1487	aureus	1482:1487	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	7	30	dep	TarS-	1233:1237	arg1	WTA					1256:1258	WTA	1256:1258	WTA	1256:1258	Using genetically modified S. aureus strains, we observed that langerin similarly recognized bacteria that produce either TarS- or TarP-modified WTA, yet tarP-expressing S. aureus induced increased cytokine production and maturation of in vitro-generated LCs compared to tarS-expressing S. aureus.					
33591717	3	31	theme	cell	413:416	arg1	cells					359:363	Langerhans cells	348:363	Langerhans cells (LCs)	348:369	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	3	31	theme	cell	413:416	arg1	type					418:421	the only professional antigen-presenting cell type	372:421	the only professional antigen-presenting cell type in the epidermis	372:438	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	10	32	dep	S.	1961:1962	arg1	aureus					1964:1969	aureus	1964:1969	aureus	1964:1969	Overall, our data suggest that LCs are able to sense all β-GlcNAc-WTA producing S. aureus strains, likely performing an important role as first responders upon S. aureus skin invasion.					
33591717	5	33	theme	subunit	996:1002	arg1	β1,3-GlcNAc					947:957	β1,3-GlcNAc	947:957	β1,3-GlcNAc	947:957	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	5	33	theme	subunit	996:1002	arg1	position					937:944	the C-3 position	929:944	the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit	929:1002	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	3	34	theme	professional	381:392	arg1	cells					359:363	Langerhans cells	348:363	Langerhans cells (LCs)	348:369	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	3	34	theme	professional	381:392	arg1	type					418:421	the only professional antigen-presenting cell type	372:421	the only professional antigen-presenting cell type in the epidermis	372:438	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	3	35	theme	receptor	491:498	arg1	langerin					500:507	their pattern-recognition receptor langerin	465:507	their pattern-recognition receptor langerin	465:507	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	9	36	theme	langerin	1627:1634	arg1	binding					1636:1642	langerin binding	1627:1642	langerin binding	1627:1642	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	2	37	theme	skin-resident	243:255	arg1	cells					264:268	skin-resident immune cells	243:268	skin-resident immune cells	243:268	It remains incompletely understood how skin-resident immune cells respond to invading S. aureus and contribute to an effective immune response.					
33591717	8	38	theme	synthesized	1420:1430	arg1	representative					1447:1460	representative	1447:1460	representative of the different S. aureus WTA glycosylation patterns	1447:1514	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	8	38	theme	synthesized	1420:1430	arg1	molecules					1436:1444	Chemically synthesized WTA molecules	1409:1444	Chemically synthesized WTA molecules	1409:1444	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	2	39	theme	immune	331:336	arg1	response					338:345	an effective immune response	318:345	an effective immune response	318:345	It remains incompletely understood how skin-resident immune cells respond to invading S. aureus and contribute to an effective immune response.					
33591717	5	40	theme	phosphate	979:987	arg1	subunit					996:1002	the WTA ribitol phosphate (RboP) subunit	963:1002	the WTA ribitol phosphate (RboP) subunit	963:1002	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	6	41	theme	langerin	1075:1082	arg1	binding					1084:1090	langerin binding	1075:1090	langerin binding	1075:1090	Here, we aimed to unravel the impact of β-GlcNAc linkage position for langerin binding and LC activation.					
33591717	4	42	theme	α-1,4-linked	628:639	arg1	modifications					662:674	α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications	628:674	α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications	628:674	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	5	43	theme	RboP	990:993	arg1	subunit					996:1002	the WTA ribitol phosphate (RboP) subunit	963:1002	the WTA ribitol phosphate (RboP) subunit	963:1002	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	6	44	theme	position	1062:1069	arg1	impact					1035:1040	the impact	1031:1040	the impact of β-GlcNAc linkage position for langerin binding and LC activation	1031:1108	Here, we aimed to unravel the impact of β-GlcNAc linkage position for langerin binding and LC activation.					
33591717	4	45	link	β-1,4-linked	573:584	arg1	β1,4-GlcNAc					607:617	β1,4-GlcNAc	607:617	β1,4-GlcNAc	607:617	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	4	45	link	β-1,4-linked	573:584	arg1	N-acetylglucosamine					586:604	the β-1,4-linked N-acetylglucosamine	569:604	the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA)	569:809	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	9	46	theme	WTA	1674:1676	arg1	molecules					1678:1686	synthetic WTA molecules	1664:1686	synthetic WTA molecules	1664:1686	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	9	46	theme	WTA	1674:1676	arg1	tool					1712:1715	a novel glycobiology tool	1691:1715	a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis	1691:1798	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	6	47	theme	β-GlcNAc	1045:1052	arg1	position					1062:1069	β-GlcNAc linkage position	1045:1069	β-GlcNAc linkage position	1045:1069	Here, we aimed to unravel the impact of β-GlcNAc linkage position for langerin binding and LC activation.					
33591717	10	48	theme	important	1921:1929	arg1	role					1931:1934	an important role	1918:1934	an important role	1918:1934	Overall, our data suggest that LCs are able to sense all β-GlcNAc-WTA producing S. aureus strains, likely performing an important role as first responders upon S. aureus skin invasion.					
33591717	10	48	theme	important	1921:1929	arg1	responders					1945:1954	first responders	1939:1954	first responders upon S. aureus skin invasion	1939:1983	Overall, our data suggest that LCs are able to sense all β-GlcNAc-WTA producing S. aureus strains, likely performing an important role as first responders upon S. aureus skin invasion.					
33591717	10	49	theme	S.	1961:1962	arg1	invasion					1976:1983	S. aureus skin invasion	1961:1983	S. aureus skin invasion	1961:1983	Overall, our data suggest that LCs are able to sense all β-GlcNAc-WTA producing S. aureus strains, likely performing an important role as first responders upon S. aureus skin invasion.					
33591717	9	50	theme	novel	1693:1697	arg1	molecules					1678:1686	synthetic WTA molecules	1664:1686	synthetic WTA molecules	1664:1686	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	9	50	theme	novel	1693:1697	arg1	tool					1712:1715	a novel glycobiology tool	1691:1715	a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis	1691:1798	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	1	51	theme	tissue	185:190	arg1	infections					192:201	soft tissue infections	180:201	soft tissue infections	180:201	Staphylococcus aureus is the leading cause of skin and soft tissue infections.					
33591717	9	52	theme	structure-binding	1721:1737	arg1	studies					1739:1745	structure-binding studies	1721:1745	structure-binding studies	1721:1745	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	10	53	theme	skin	1971:1974	arg1	invasion					1976:1983	S. aureus skin invasion	1961:1983	S. aureus skin invasion	1961:1983	Overall, our data suggest that LCs are able to sense all β-GlcNAc-WTA producing S. aureus strains, likely performing an important role as first responders upon S. aureus skin invasion.					
33591717	8	54	theme	S.	1479:1480	arg1	patterns					1507:1514	the different S. aureus WTA glycosylation patterns	1465:1514	the different S. aureus WTA glycosylation patterns	1465:1514	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	7	55	theme	LCs	1366:1368	arg1	maturation					1333:1342	maturation	1333:1342	maturation	1333:1342	Using genetically modified S. aureus strains, we observed that langerin similarly recognized bacteria that produce either TarS- or TarP-modified WTA, yet tarP-expressing S. aureus induced increased cytokine production and maturation of in vitro-generated LCs compared to tarS-expressing S. aureus.					
33591717	7	55	theme	LCs	1366:1368	arg1	production					1318:1327	cytokine production	1309:1327	cytokine production	1309:1327	Using genetically modified S. aureus strains, we observed that langerin similarly recognized bacteria that produce either TarS- or TarP-modified WTA, yet tarP-expressing S. aureus induced increased cytokine production and maturation of in vitro-generated LCs compared to tarS-expressing S. aureus.					
33591717	7	56	theme	modified	1129:1136	arg1	strains					1148:1154	genetically modified S. aureus strains	1117:1154	genetically modified S. aureus strains	1117:1154	Using genetically modified S. aureus strains, we observed that langerin similarly recognized bacteria that produce either TarS- or TarP-modified WTA, yet tarP-expressing S. aureus induced increased cytokine production and maturation of in vitro-generated LCs compared to tarS-expressing S. aureus.					
33591717	8	57	theme	WTA	1489:1491	arg1	patterns					1507:1514	the different S. aureus WTA glycosylation patterns	1465:1514	the different S. aureus WTA glycosylation patterns	1465:1514	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	7	58	theme	in	1347:1348	arg1	LCs					1366:1368	in vitro-generated LCs	1347:1368	in vitro-generated LCs	1347:1368	Using genetically modified S. aureus strains, we observed that langerin similarly recognized bacteria that produce either TarS- or TarP-modified WTA, yet tarP-expressing S. aureus induced increased cytokine production and maturation of in vitro-generated LCs compared to tarS-expressing S. aureus.					
33591717	2	59	theme	immune	257:262	arg1	cells					264:268	skin-resident immune cells	243:268	skin-resident immune cells	243:268	It remains incompletely understood how skin-resident immune cells respond to invading S. aureus and contribute to an effective immune response.					
33591717	8	60	theme	patterns	1507:1514	arg1	representative					1447:1460	representative	1447:1460	representative of the different S. aureus WTA glycosylation patterns	1447:1514	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	8	60	theme	patterns	1507:1514	arg1	molecules					1436:1444	Chemically synthesized WTA molecules	1409:1444	Chemically synthesized WTA molecules	1409:1444	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	4	61	theme	glycosyltransferases	706:725	arg1	TarS					727:730	dedicated glycosyltransferases TarS	696:730	dedicated glycosyltransferases TarS	696:730	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	0	62	theme	Langerin	72:79	arg1	Recognition					81:91	Langerin Recognition	72:91	Langerin Recognition	72:91	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	1	63	theme	leading	154:160	arg1	aureus					140:145	Staphylococcus aureus	125:145	Staphylococcus aureus	125:145	Staphylococcus aureus is the leading cause of skin and soft tissue infections.					
33591717	1	63	theme	leading	154:160	arg1	cause					162:166	the leading cause	150:166	the leading cause of skin and soft tissue infections	150:201	Staphylococcus aureus is the leading cause of skin and soft tissue infections.					
33591717	9	64	dep	S.	1767:1768	arg1	aureus					1770:1775	aureus	1770:1775	aureus	1770:1775	We established that β-GlcNAc is sufficient to confer langerin binding, thereby presenting synthetic WTA molecules as a novel glycobiology tool for structure-binding studies and for elucidating S. aureus molecular pathogenesis.					
33591717	3	65	from	type	418:421	arg1	epidermis					430:438	the epidermis	426:438	the epidermis	426:438	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	4	66	theme	wall	786:789	arg1	WTA					806:808	WTA	806:808	WTA	806:808	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	4	66	theme	wall	786:789	arg1	acid					800:803	glycopolymer wall teichoic acid	773:803	the cell wall glycopolymer wall teichoic acid (WTA)	759:809	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	3	67	theme	pattern-recognition	471:489	arg1	langerin					500:507	their pattern-recognition receptor langerin	465:507	their pattern-recognition receptor langerin	465:507	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	0	68	theme	Cell	108:111	arg1	Activation					113:122	Langerhans Cell Activation	97:122	Langerhans Cell Activation	97:122	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	7	69	theme	cytokine	1309:1316	arg1	production					1318:1327	cytokine production	1309:1327	cytokine production	1309:1327	Using genetically modified S. aureus strains, we observed that langerin similarly recognized bacteria that produce either TarS- or TarP-modified WTA, yet tarP-expressing S. aureus induced increased cytokine production and maturation of in vitro-generated LCs compared to tarS-expressing S. aureus.					
33591717	8	70	theme	langerin-WTA	1539:1550	arg1	requirements					1560:1571	langerin-WTA binding requirements	1539:1571	langerin-WTA binding requirements	1539:1571	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	7	71	theme	aureus	1141:1146	arg1	strains					1148:1154	genetically modified S. aureus strains	1117:1154	genetically modified S. aureus strains	1117:1154	Using genetically modified S. aureus strains, we observed that langerin similarly recognized bacteria that produce either TarS- or TarP-modified WTA, yet tarP-expressing S. aureus induced increased cytokine production and maturation of in vitro-generated LCs compared to tarS-expressing S. aureus.					
33591717	5	72	theme	C-3	933:935	arg1	β1,3-GlcNAc					947:957	β1,3-GlcNAc	947:957	β1,3-GlcNAc	947:957	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	5	72	theme	C-3	933:935	arg1	position					937:944	the C-3 position	929:944	the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit	929:1002	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	4	73	theme	β-1,4-linked	573:584	arg1	β1,4-GlcNAc					607:617	β1,4-GlcNAc	607:617	β1,4-GlcNAc	607:617	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	4	73	theme	β-1,4-linked	573:584	arg1	N-acetylglucosamine					586:604	the β-1,4-linked N-acetylglucosamine	569:604	the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA)	569:809	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	0	74	theme	Linkage	17:23	arg1	Impact					0:5	Impact	0:5	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.	0:123	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	0	75	theme	Staphylococcus	28:41	arg1	Acid					64:67	Staphylococcus aureus Wall Teichoic Acid	28:67	Staphylococcus aureus Wall Teichoic Acid	28:67	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	10	76	theme	S.	1881:1882	arg1	strains					1891:1897	S. aureus strains	1881:1897	S. aureus strains	1881:1897	Overall, our data suggest that LCs are able to sense all β-GlcNAc-WTA producing S. aureus strains, likely performing an important role as first responders upon S. aureus skin invasion.					
33591717	0	77	theme	Wall	50:53	arg1	Acid					64:67	Staphylococcus aureus Wall Teichoic Acid	28:67	Staphylococcus aureus Wall Teichoic Acid	28:67	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	4	78	link	α-1,4-linked	628:639	arg1	modifications					662:674	α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications	628:674	α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications	628:674	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	0	79	dep	Staphylococcus	28:41	arg1	aureus					43:48	aureus	43:48	aureus	43:48	Impact of Glycan Linkage to Staphylococcus aureus Wall Teichoic Acid on Langerin Recognition and Langerhans Cell Activation.					
33591717	6	80	theme	LC	1096:1097	arg1	activation					1099:1108	LC activation	1096:1108	LC activation	1096:1108	Here, we aimed to unravel the impact of β-GlcNAc linkage position for langerin binding and LC activation.					
33591717	3	81	theme	antigen-presenting	394:411	arg1	cells					359:363	Langerhans cells	348:363	Langerhans cells (LCs)	348:369	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	3	81	theme	antigen-presenting	394:411	arg1	type					418:421	the only professional antigen-presenting cell type	372:421	the only professional antigen-presenting cell type in the epidermis	372:438	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	5	82	theme	ribitol	971:977	arg1	subunit					996:1002	the WTA ribitol phosphate (RboP) subunit	963:1002	the WTA ribitol phosphate (RboP) subunit	963:1002	Recently, an alternative WTA glycosyltransferase, TarP, was identified, which also modifies WTA with β-GlcNAc but at the C-3 position (β1,3-GlcNAc) of the WTA ribitol phosphate (RboP) subunit.					
33591717	4	83	theme	GlcNAc	641:646	arg1	modifications					662:674	α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications	628:674	α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications	628:674	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	2	84	theme	effective	321:329	arg1	response					338:345	an effective immune response	318:345	an effective immune response	318:345	It remains incompletely understood how skin-resident immune cells respond to invading S. aureus and contribute to an effective immune response.					
33591717	4	85	theme	α1,4-GlcNAc	649:659	arg1	modifications					662:674	α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications	628:674	α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications	628:674	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
33591717	10	86	theme	first	1939:1943	arg1	role					1931:1934	an important role	1918:1934	an important role	1918:1934	Overall, our data suggest that LCs are able to sense all β-GlcNAc-WTA producing S. aureus strains, likely performing an important role as first responders upon S. aureus skin invasion.					
33591717	10	86	theme	first	1939:1943	arg1	responders					1945:1954	first responders	1939:1954	first responders upon S. aureus skin invasion	1939:1983	Overall, our data suggest that LCs are able to sense all β-GlcNAc-WTA producing S. aureus strains, likely performing an important role as first responders upon S. aureus skin invasion.					
33591717	8	87	theme	WTA	1432:1434	arg1	representative					1447:1460	representative	1447:1460	representative of the different S. aureus WTA glycosylation patterns	1447:1514	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	8	87	theme	WTA	1432:1434	arg1	molecules					1436:1444	Chemically synthesized WTA molecules	1409:1444	Chemically synthesized WTA molecules	1409:1444	Chemically synthesized WTA molecules, representative of the different S. aureus WTA glycosylation patterns, were used to identify langerin-WTA binding requirements.					
33591717	3	88	theme	proinflammatory	523:537	arg1	response					539:546	a proinflammatory response	521:546	a proinflammatory response	521:546	Langerhans cells (LCs), the only professional antigen-presenting cell type in the epidermis, sense S. aureus through their pattern-recognition receptor langerin, triggering a proinflammatory response.					
33591717	4	89	from	TarM	736:739	arg1	wall					768:771	the cell wall glycopolymer wall teichoic acid (WTA)	759:809	the cell wall glycopolymer wall teichoic acid (WTA)	759:809	Langerin recognizes the β-1,4-linked N-acetylglucosamine (β1,4-GlcNAc) but not α-1,4-linked GlcNAc (α1,4-GlcNAc) modifications, which are added by dedicated glycosyltransferases TarS and TarM, respectively, on the cell wall glycopolymer wall teichoic acid (WTA).					
30099598	2	0	theme	other	653:657	arg1	reagents					668:675	other research reagents	653:675	other research reagents	653:675	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	1	dep	engineer	304:311	arg1	used					322:325	used	322:325	to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways	301:508	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	3	2	theme	such	893:896	arg1	products					898:905	such products	893:905	such products	893:905	This chapter highlights some of the key discoveries and technologies for equipping bacteria with the requisite biosynthetic machinery to generate such products.					
30099598	2	3	theme	facile	530:535	arg1	generation					537:546	the facile generation	526:546	the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents	526:675	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	4	from	pathways	501:508	arg1	enzymes					454:460	enzymes	454:460	enzymes from various carbohydrate biosynthesis pathways	454:508	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	4	from	pathways	501:508	arg1	expression					440:449	recombinant expression	428:449	recombinant expression of enzymes from various carbohydrate biosynthesis pathways	428:508	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	4	5	theme	bacterial	915:923	arg1	glyco-toolbox					925:937	the bacterial glyco-toolbox	911:937	the bacterial glyco-toolbox	911:937	As the bacterial glyco-toolbox continues to grow, these technologies are expected to expand the range of glycomolecules produced recombinantly in bacterial systems, thereby opening up this platform to an even larger number of applications.					
30099598	2	6	theme	conjugate	578:586	arg1	vaccines					588:595	conjugate vaccines	578:595	conjugate vaccines	578:595	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	3	7	with	discoveries	787:797	arg1	machinery					871:879	the requisite biosynthetic machinery	844:879	the requisite biosynthetic machinery	844:879	This chapter highlights some of the key discoveries and technologies for equipping bacteria with the requisite biosynthetic machinery to generate such products.					
30099598	2	8	theme	recombinant	428:438	arg1	expression					440:449	recombinant expression	428:449	recombinant expression of enzymes from various carbohydrate biosynthesis pathways	428:508	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	9	theme	products	561:568	arg1	generation					537:546	the facile generation	526:546	the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents	526:675	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	10	theme	living	731:736	arg1	systems					738:744	living systems	731:744	living systems	731:744	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	0	11	theme	Bacterial	0:8	arg1	Glycoengineering					10:25	Bacterial Glycoengineering	0:25	Bacterial Glycoengineering	0:25	Bacterial Glycoengineering as a Biosynthetic Route to Customized Glycomolecules.					
30099598	2	12	theme	non-native	397:406	arg1	structures					414:423	non-native sugar structures	397:423	non-native sugar structures	397:423	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	4	13	theme	bacterial	1054:1062	arg1	systems					1064:1070	bacterial systems	1054:1070	bacterial systems	1054:1070	As the bacterial glyco-toolbox continues to grow, these technologies are expected to expand the range of glycomolecules produced recombinantly in bacterial systems, thereby opening up this platform to an even larger number of applications.					
30099598	2	14	theme	reagents	668:675	arg1	vesicles					626:633	glycosylated outer membrane vesicles	598:633	glycosylated outer membrane vesicles	598:633	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	14	theme	reagents	668:675	arg1	vaccines					588:595	conjugate vaccines	578:595	conjugate vaccines	578:595	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	14	theme	reagents	668:675	arg1	variety					642:648	a variety	640:648	a variety of other research reagents	640:675	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	14	theme	reagents	668:675	arg1	reagents					668:675	other research reagents	653:675	other research reagents	653:675	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	15	theme	laboratory	327:336	arg1	coli					366:369	Escherichia coli	354:369	Escherichia coli	354:369	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	15	theme	laboratory	327:336	arg1	species					338:344	laboratory species	327:344	laboratory species such as Escherichia coli	327:369	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	1	16	theme	variety	179:185	arg1	biosynthesis					161:172	the biosynthesis	157:172	the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins	157:286	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	2	17	theme	biosynthesis	488:499	arg1	pathways					501:508	various carbohydrate biosynthesis pathways	467:508	various carbohydrate biosynthesis pathways	467:508	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	18	theme	research	659:666	arg1	reagents					668:675	other research reagents	653:675	other research reagents	653:675	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	19	theme	glycans	720:726	arg1	role					712:715	the role	708:715	the role of glycans in living systems	708:744	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	1	20	theme	increased	104:112	arg1	interest					114:121	increased interest	104:121	increased interest in recent years	104:137	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	1	20	theme	increased	104:112	arg1	platform					144:151	a platform	142:151	a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins	142:286	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	2	21	theme	sugar	408:412	arg1	structures					414:423	non-native sugar structures	397:423	non-native sugar structures	397:423	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	22	theme	carbohydrate	475:486	arg1	pathways					501:508	various carbohydrate biosynthesis pathways	467:508	various carbohydrate biosynthesis pathways	467:508	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	23	theme	outer	611:615	arg1	vesicles					626:633	glycosylated outer membrane vesicles	598:633	glycosylated outer membrane vesicles	598:633	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	1	24	gly	glycoproteins	274:286	arg1	glycoproteins					274:286	glycoproteins	274:286	glycoproteins	274:286	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	1	25	theme	glycomolecules	190:203	arg1	variety					179:185	a variety	177:185	a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins	177:286	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	1	25	theme	glycomolecules	190:203	arg1	oligosaccharides					221:236	soluble oligosaccharides	213:236	soluble oligosaccharides	213:236	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	1	25	theme	glycomolecules	190:203	arg1	carbohydrates					255:267	surface-exposed carbohydrates	239:267	surface-exposed carbohydrates	239:267	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	1	25	theme	glycomolecules	190:203	arg1	glycoproteins					274:286	glycoproteins	274:286	glycoproteins	274:286	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	1	25	theme	glycomolecules	190:203	arg1	glycomolecules					190:203	glycomolecules	190:203	glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins	190:286	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	2	26	theme	various	467:473	arg1	pathways					501:508	various carbohydrate biosynthesis pathways	467:508	various carbohydrate biosynthesis pathways	467:508	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	3	27	theme	key	783:785	arg1	discoveries					787:797	the key discoveries	779:797	the key discoveries	779:797	This chapter highlights some of the key discoveries and technologies for equipping bacteria with the requisite biosynthetic machinery to generate such products.					
30099598	2	28	theme	glycosylated	598:609	arg1	vesicles					626:633	glycosylated outer membrane vesicles	598:633	glycosylated outer membrane vesicles	598:633	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	29	theme	enzymes	454:460	arg1	expression					440:449	recombinant expression	428:449	recombinant expression of enzymes from various carbohydrate biosynthesis pathways	428:508	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	0	30	theme	Biosynthetic	32:43	arg1	Route					45:49	a Biosynthetic Route	30:49	a Biosynthetic Route to Customized Glycomolecules	30:78	Bacterial Glycoengineering as a Biosynthetic Route to Customized Glycomolecules.					
30099598	4	31	dep	platform	1097:1104	arg1	up					1089:1090	up	1089:1090	up	1089:1090	As the bacterial glyco-toolbox continues to grow, these technologies are expected to expand the range of glycomolecules produced recombinantly in bacterial systems, thereby opening up this platform to an even larger number of applications.					
30099598	1	32	theme	soluble	213:219	arg1	oligosaccharides					221:236	soluble oligosaccharides	213:236	soluble oligosaccharides	213:236	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	0	33	theme	Customized	54:63	arg1	Glycomolecules					65:78	Customized Glycomolecules	54:78	Customized Glycomolecules	54:78	Bacterial Glycoengineering as a Biosynthetic Route to Customized Glycomolecules.					
30099598	1	34	theme	recent	126:131	arg1	years					133:137	recent years	126:137	recent years	126:137	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	2	35	dep	ability	293:299	arg1	engineer					304:311	engineer	304:311	to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways	301:508	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	1	36	from	interest	114:121	arg1	years					133:137	recent years	126:137	recent years	126:137	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	3	37	theme	equipping	820:828	arg1	bacteria					830:837	equipping bacteria	820:837	equipping bacteria	820:837	This chapter highlights some of the key discoveries and technologies for equipping bacteria with the requisite biosynthetic machinery to generate such products.					
30099598	2	38	from	role	712:715	arg1	systems					738:744	living systems	731:744	living systems	731:744	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	4	39	theme	applications	1134:1145	arg1	number					1124:1129	an even larger number	1109:1129	an even larger number of applications	1109:1145	As the bacterial glyco-toolbox continues to grow, these technologies are expected to expand the range of glycomolecules produced recombinantly in bacterial systems, thereby opening up this platform to an even larger number of applications.					
30099598	3	40	theme	biosynthetic	858:869	arg1	machinery					871:879	the requisite biosynthetic machinery	844:879	the requisite biosynthetic machinery	844:879	This chapter highlights some of the key discoveries and technologies for equipping bacteria with the requisite biosynthetic machinery to generate such products.					
30099598	2	41	theme	membrane	617:624	arg1	vesicles					626:633	glycosylated outer membrane vesicles	598:633	glycosylated outer membrane vesicles	598:633	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	3	42	with	technologies	803:814	arg1	machinery					871:879	the requisite biosynthetic machinery	844:879	the requisite biosynthetic machinery	844:879	This chapter highlights some of the key discoveries and technologies for equipping bacteria with the requisite biosynthetic machinery to generate such products.					
30099598	2	43	theme	important	551:559	arg1	vesicles					626:633	glycosylated outer membrane vesicles	598:633	glycosylated outer membrane vesicles	598:633	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	43	theme	important	551:559	arg1	vaccines					588:595	conjugate vaccines	578:595	conjugate vaccines	578:595	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	43	theme	important	551:559	arg1	variety					642:648	a variety	640:648	a variety of other research reagents	640:675	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	43	theme	important	551:559	arg1	products					561:568	important products	551:568	important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents	551:675	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	43	theme	important	551:559	arg1	reagents					668:675	other research reagents	653:675	other research reagents	653:675	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	0	44	dep	Glycoengineering	10:25	arg1	Route					45:49	a Biosynthetic Route	30:49	a Biosynthetic Route to Customized Glycomolecules	30:78	Bacterial Glycoengineering as a Biosynthetic Route to Customized Glycomolecules.					
30099598	2	45	from	expression	440:449	arg1	pathways					501:508	various carbohydrate biosynthesis pathways	467:508	various carbohydrate biosynthesis pathways	467:508	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	2	46	gly	glycosylated	598:609	arg1	vesicles					626:633	glycosylated outer membrane vesicles	598:633	glycosylated outer membrane vesicles	598:633	The ability to engineer commonly used laboratory species such as Escherichia coli to efficiently synthesize non-native sugar structures by recombinant expression of enzymes from various carbohydrate biosynthesis pathways has allowed for the facile generation of important products such as conjugate vaccines, glycosylated outer membrane vesicles, and a variety of other research reagents for studying and understanding the role of glycans in living systems.					
30099598	1	47	theme	surface-exposed	239:253	arg1	carbohydrates					255:267	surface-exposed carbohydrates	239:267	surface-exposed carbohydrates	239:267	Bacteria have garnered increased interest in recent years as a platform for the biosynthesis of a variety of glycomolecules such as soluble oligosaccharides, surface-exposed carbohydrates, and glycoproteins.					
30099598	4	48	theme	glycomolecules	1013:1026	arg1	range					1004:1008	the range	1000:1008	the range of glycomolecules produced recombinantly in bacterial systems, thereby opening up this platform to an even larger number of applications	1000:1145	As the bacterial glyco-toolbox continues to grow, these technologies are expected to expand the range of glycomolecules produced recombinantly in bacterial systems, thereby opening up this platform to an even larger number of applications.					
30099598	4	49	theme	larger	1117:1122	arg1	number					1124:1129	an even larger number	1109:1129	an even larger number of applications	1109:1145	As the bacterial glyco-toolbox continues to grow, these technologies are expected to expand the range of glycomolecules produced recombinantly in bacterial systems, thereby opening up this platform to an even larger number of applications.					
30099598	3	50	theme	requisite	848:856	arg1	machinery					871:879	the requisite biosynthetic machinery	844:879	the requisite biosynthetic machinery	844:879	This chapter highlights some of the key discoveries and technologies for equipping bacteria with the requisite biosynthetic machinery to generate such products.					
34529436	2	0	theme	antibodies	462:471	arg1	variety					438:444	a variety	436:444	a variety of neutralizing antibodies	436:471	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	2	0	theme	antibodies	462:471	arg1	antibodies					462:471	neutralizing antibodies	449:471	neutralizing antibodies	449:471	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	6	1	from	modes	1098:1102	arg1	motion					1142:1147	the motion	1138:1147	the motion of S protein receptor binding domains	1138:1185	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	6	2	theme	binding	1090:1096	arg1	modes					1098:1102	the antibody binding modes	1077:1102	the antibody binding modes	1077:1102	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	7	3	theme	vaccine	1448:1454	arg1	development					1433:1443	the development	1429:1443	the development of vaccine and antibody-based therapy	1429:1481	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	2	4	theme	neutralizing	449:460	arg1	antibodies					462:471	neutralizing antibodies	449:471	neutralizing antibodies	449:471	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	3	5	theme	molecular	652:660	arg1	dynamics					662:669	all-atom molecular dynamics	643:669	all-atom molecular dynamics simulations	643:681	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	6	6	theme	antibody	1081:1088	arg1	modes					1098:1102	the antibody binding modes	1077:1102	the antibody binding modes	1077:1102	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	1	7	theme	potent	241:246	arg1	antibodies					261:270	highly potent neutralizing antibodies	234:270	highly potent neutralizing antibodies	234:270	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	3	8	theme	dynamics	662:669	arg1	simulations					671:681	all-atom molecular dynamics simulations	643:681	all-atom molecular dynamics simulations	643:681	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	4	9	theme	potential	866:874	arg1	influence					876:884	the potential influence	862:884	the potential influence of mutations in SARS-CoV-2 variants	862:920	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.					
34529436	6	10	theme	S	1152:1152	arg1	domains					1179:1185	S protein receptor binding domains	1152:1185	S protein receptor binding domains	1152:1185	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	3	11	theme	S	505:505	arg1	structures					531:540	various S trimer-antibody complex structures	497:540	various S trimer-antibody complex structures	497:540	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	5	12	theme	conformations	948:960	arg1	Comparison					923:932	Comparison	923:932	Comparison of the glycan conformations between S-only and S-antibody systems	923:998	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.					
34529436	1	13	theme	neutralizing	248:259	arg1	antibodies					261:270	highly potent neutralizing antibodies	234:270	highly potent neutralizing antibodies	234:270	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	7	14	theme	interactions	1255:1266	arg1	understanding					1227:1239	a better understanding	1218:1239	a better understanding of S-antibody interactions	1218:1266	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	5	15	dep	S-only	970:975	arg1	systems					992:998	systems	992:998	systems	992:998	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.					
34529436	6	16	theme	receptor	1162:1169	arg1	domains					1179:1185	S protein receptor binding domains	1152:1185	S protein receptor binding domains	1152:1185	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	3	17	theme	trimer-antibody	507:521	arg1	structures					531:540	various S trimer-antibody complex structures	497:540	various S trimer-antibody complex structures	497:540	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	2	18	theme	spike	315:319	arg1	protein					325:331	The spike (S) protein	311:331	The spike (S) protein on the viral envelope	311:353	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	6	19	theme	antibody	1126:1133	arg1	modes					1098:1102	the antibody binding modes	1077:1102	the antibody binding modes	1077:1102	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	6	19	theme	antibody	1126:1133	arg1	influences					1112:1121	the influences	1108:1121	the influences of antibody on the motion of S protein receptor binding domains	1108:1185	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	6	20	theme	protein	1154:1160	arg1	domains					1179:1185	S protein receptor binding domains	1152:1185	S protein receptor binding domains	1152:1185	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	5	21	from	roles	1012:1016	arg1	binding					1043:1049	S-antibody binding	1032:1049	S-antibody binding	1032:1049	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.					
34529436	2	22	theme	angiotensin-converting	370:391	arg1	enzyme					393:398	human angiotensin-converting enzyme 2	364:400	human angiotensin-converting enzyme 2 binding	364:408	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	4	23	theme	S-antibody	822:831	arg1	binding					833:839	S-antibody binding	822:839	S-antibody binding	822:839	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.					
34529436	3	24	theme	various	497:503	arg1	structures					531:540	various S trimer-antibody complex structures	497:540	various S trimer-antibody complex structures	497:540	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	7	25	from	influences	1351:1360	arg1	interactions					1390:1401	S-antibody interactions	1379:1401	S-antibody interactions	1379:1401	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	2	26	theme	human	364:368	arg1	enzyme					393:398	human angiotensin-converting enzyme 2	364:400	human angiotensin-converting enzyme 2 binding	364:408	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	2	27	from	protein	325:331	arg1	envelope					346:353	the viral envelope	336:353	the viral envelope	336:353	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	1	28	theme	public	179:184	arg1	crisis					193:198	a public health crisis	177:198	a public health crisis	177:198	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	0	29	theme	Dynamic	0:6	arg1	Interactions					8:19	Dynamic Interactions	0:19	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.	0:91	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.					
34529436	7	30	theme	better	1220:1225	arg1	understanding					1227:1239	a better understanding	1218:1239	a better understanding of S-antibody interactions	1218:1266	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	2	31	dep	spike	315:319	arg1	S					322:322	S	322:322	S	322:322	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	1	32	theme	health	186:191	arg1	crisis					193:198	a public health crisis	177:198	a public health crisis	177:198	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	5	33	theme	S-antibody	1032:1041	arg1	binding					1043:1049	S-antibody binding	1032:1049	S-antibody binding	1032:1049	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.					
34529436	3	34	theme	complex	523:529	arg1	structures					531:540	various S trimer-antibody complex structures	497:540	various S trimer-antibody complex structures	497:540	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	7	35	theme	interaction	1305:1315	arg1	maps					1317:1320	the simulation-based S-antibody interaction maps	1273:1320	the simulation-based S-antibody interaction maps	1273:1320	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	3	36	theme	structural	708:717	arg1	dynamics					719:726	the structural dynamics	704:726	the structural dynamics	704:726	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	4	37	theme	SARS-CoV-2	902:911	arg1	variants					913:920	SARS-CoV-2 variants	902:920	SARS-CoV-2 variants	902:920	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.					
34529436	6	38	theme	binding	1171:1177	arg1	domains					1179:1185	S protein receptor binding domains	1152:1185	S protein receptor binding domains	1152:1185	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	1	39	theme	severe	107:112	arg1	coronavirus					141:151	severe acute respiratory syndrome coronavirus 2	107:153	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	107:166	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	1	39	theme	severe	107:112	arg1	SARS-CoV-2					156:165	SARS-CoV-2	156:165	SARS-CoV-2	156:165	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	0	40	theme	SARS-CoV-2	43:52	arg1	Protein					60:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.					
34529436	7	41	theme	S-antibody	1294:1303	arg1	maps					1317:1320	the simulation-based S-antibody interaction maps	1273:1320	the simulation-based S-antibody interaction maps	1273:1320	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	3	42	theme	previous	615:622	arg1	work					624:627	our previous work	611:627	our previous work	611:627	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	4	43	theme	residues	800:807	arg1	Investigation					779:791	Investigation	779:791	Investigation of the residues critical for S-antibody binding	779:839	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.					
34529436	3	44	gly	glycosylated	568:579	arg1	models					591:596	the fully glycosylated S protein models	558:596	the fully glycosylated S protein models described in our previous work	558:627	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	7	45	theme	simulation-based	1277:1292	arg1	maps					1317:1320	the simulation-based S-antibody interaction maps	1273:1320	the simulation-based S-antibody interaction maps	1273:1320	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	2	46	theme	variety	438:444	arg1	target					426:431	the target	422:431	the target of a variety of neutralizing antibodies	422:471	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	7	47	theme	antibody-based	1460:1473	arg1	therapy					1475:1481	antibody-based therapy	1460:1481	antibody-based therapy	1460:1481	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	0	48	theme	Glycosylated	30:41	arg1	Protein					60:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.					
34529436	5	49	theme	glycans	1021:1027	arg1	roles					1012:1016	the roles	1008:1016	the roles of glycans in S-antibody binding	1008:1049	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.					
34529436	6	50	from	influences	1112:1121	arg1	motion					1142:1147	the motion	1138:1147	the motion of S protein receptor binding domains	1138:1185	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	2	51	theme	enzyme	393:398	arg1	binding					402:408	human angiotensin-converting enzyme 2 binding	364:408	human angiotensin-converting enzyme 2 binding	364:408	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	0	52	theme	Protein	60:66	arg1	Interactions					8:19	Dynamic Interactions	0:19	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.	0:91	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.					
34529436	7	53	theme	S	1365:1365	arg1	mutation					1367:1374	S mutation	1365:1374	S mutation	1365:1374	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	1	54	theme	acute	114:118	arg1	coronavirus					141:151	severe acute respiratory syndrome coronavirus 2	107:153	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	107:166	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	1	54	theme	acute	114:118	arg1	SARS-CoV-2					156:165	SARS-CoV-2	156:165	SARS-CoV-2	156:165	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	0	55	theme	Spike	54:58	arg1	Protein					60:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Fully Glycosylated SARS-CoV-2 Spike Protein	24:66	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.					
34529436	4	56	theme	critical	809:816	arg1	residues					800:807	the residues	796:807	the residues critical for S-antibody binding	796:839	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.					
34529436	0	57	with	Interactions	8:19	arg1	Antibodies					81:90	Various Antibodies	73:90	Various Antibodies	73:90	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.					
34529436	1	58	theme	respiratory	120:130	arg1	coronavirus					141:151	severe acute respiratory syndrome coronavirus 2	107:153	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	107:166	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	1	58	theme	respiratory	120:130	arg1	SARS-CoV-2					156:165	SARS-CoV-2	156:165	SARS-CoV-2	156:165	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	7	59	theme	therapy	1475:1481	arg1	development					1433:1443	the development	1429:1443	the development of vaccine and antibody-based therapy	1429:1481	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	3	60	theme	S	753:753	arg1	protein					755:761	S protein	753:761	S protein	753:761	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	3	61	theme	all-atom	643:650	arg1	dynamics					662:669	all-atom molecular dynamics	643:669	all-atom molecular dynamics simulations	643:681	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	3	62	theme	protein	583:589	arg1	models					591:596	the fully glycosylated S protein models	558:596	the fully glycosylated S protein models described in our previous work	558:627	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	7	63	theme	S-antibody	1379:1388	arg1	interactions					1390:1401	S-antibody interactions	1379:1401	S-antibody interactions	1379:1401	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	1	64	theme	syndrome	132:139	arg1	coronavirus					141:151	severe acute respiratory syndrome coronavirus 2	107:153	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	107:166	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	1	64	theme	syndrome	132:139	arg1	SARS-CoV-2					156:165	SARS-CoV-2	156:165	SARS-CoV-2	156:165	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	5	65	theme	glycan	941:946	arg1	conformations					948:960	the glycan conformations	937:960	the glycan conformations	937:960	Comparison of the glycan conformations between S-only and S-antibody systems reveals the roles of glycans in S-antibody binding.					
34529436	4	66	theme	mutations	889:897	arg1	influence					876:884	the potential influence	862:884	the potential influence of mutations in SARS-CoV-2 variants	862:920	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.					
34529436	3	67	theme	models	591:596	arg1	basis					549:553	the basis	545:553	the basis of the fully glycosylated S protein models described in our previous work	545:627	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	1	68	theme	coronavirus	141:151	arg1	spread					97:102	The spread	93:102	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	93:166	The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a public health crisis, and the vaccines that can induce highly potent neutralizing antibodies are essential for ending the pandemic.					
34529436	6	69	theme	domains	1179:1185	arg1	motion					1142:1147	the motion	1138:1147	the motion of S protein receptor binding domains	1138:1185	In addition, we explored the antibody binding modes and the influences of antibody on the motion of S protein receptor binding domains.					
34529436	0	70	theme	Various	73:79	arg1	Antibodies					81:90	Various Antibodies	73:90	Various Antibodies	73:90	Dynamic Interactions of Fully Glycosylated SARS-CoV-2 Spike Protein with Various Antibodies.					
34529436	4	71	from	influence	876:884	arg1	variants					913:920	SARS-CoV-2 variants	902:920	SARS-CoV-2 variants	902:920	Investigation of the residues critical for S-antibody binding allows us to predict the potential influence of mutations in SARS-CoV-2 variants.					
34529436	3	72	theme	glycosylated	568:579	arg1	models					591:596	the fully glycosylated S protein models	558:596	the fully glycosylated S protein models described in our previous work	558:627	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	2	73	theme	viral	340:344	arg1	envelope					346:353	the viral envelope	336:353	the viral envelope	336:353	The spike (S) protein on the viral envelope mediates human angiotensin-converting enzyme 2 binding and thus is the target of a variety of neutralizing antibodies.					
34529436	3	74	theme	S	581:581	arg1	models					591:596	the fully glycosylated S protein models	558:596	the fully glycosylated S protein models described in our previous work	558:627	In this work, we built various S trimer-antibody complex structures on the basis of the fully glycosylated S protein models described in our previous work and performed all-atom molecular dynamics simulations to gain insight into the structural dynamics and interactions between S protein and antibodies.					
34529436	7	75	used	used	1331:1334	arg2	maps					1317:1320	the simulation-based S-antibody interaction maps	1273:1320	the simulation-based S-antibody interaction maps	1273:1320	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	7	76	theme	S-antibody	1244:1253	arg1	interactions					1255:1266	S-antibody interactions	1244:1266	S-antibody interactions	1244:1266	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
34529436	7	77	theme	mutation	1367:1374	arg1	influences					1351:1360	the influences	1347:1360	the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy	1347:1481	Overall, our analyses provide a better understanding of S-antibody interactions, and the simulation-based S-antibody interaction maps could be used to predict the influences of S mutation on S-antibody interactions, which will be useful for the development of vaccine and antibody-based therapy.					
31898491	12	0	theme	adhesion	1872:1879	arg1	complex					1881:1887	paxillin/FAK adhesion complex	1859:1887	paxillin/FAK adhesion complex	1859:1887	SRGN/CD44 interaction promotes focal adhesion turnover via Src-mediated paxillin phosphorylation and disassembly of paxillin/FAK adhesion complex, facilitating cell migration.					
31898491	10	1	theme	cell	1581:1584	arg1	CD44					1594:1597	tumor cell surface CD44	1575:1597	tumor cell surface CD44	1575:1597	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	7	2	theme	interest	1193:1200	arg1	proteins					1181:1188	proteins	1181:1188	proteins of interest	1181:1200	Western blot and co-immunoprecipitation analyses were performed to determine the expression and interaction of proteins of interest.					
31898491	0	3	with	interaction	86:96	arg1	CD44					129:132	CD44	129:132	CD44	129:132	Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44.					
31898491	1	4	theme	granules	266:273	arg1	processes					243:251	the storage processes	231:251	the storage processes of secretory granules	231:273	BACKGROUND Serglycin (SRGN), previously recognized as an intracellular proteoglycan involved in the storage processes of secretory granules, has recently been shown to be upregulated in several solid tumors.					
31898491	6	5	theme	chromatography/tandem	924:944	arg1	spectrometry					951:962	Liquid chromatography/tandem mass spectrometry	917:962	Liquid chromatography/tandem mass spectrometry	917:962	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	12	6	theme	paxillin	1815:1822	arg1	phosphorylation					1824:1838	Src-mediated paxillin phosphorylation	1802:1838	Src-mediated paxillin phosphorylation	1802:1838	SRGN/CD44 interaction promotes focal adhesion turnover via Src-mediated paxillin phosphorylation and disassembly of paxillin/FAK adhesion complex, facilitating cell migration.					
31898491	17	7	theme	specific	2546:2553	arg1	glycans					2555:2561	specific glycans	2546:2561	specific glycans	2546:2561	These findings suggest that targeting specific glycans in tumor microenvironment that serve as ligands for oncogenic pathways may be a potential strategy for cancer therapy.					
31898491	7	8	theme	proteins	1181:1188	arg1	interaction					1166:1176	interaction	1166:1176	interaction	1166:1176	Western blot and co-immunoprecipitation analyses were performed to determine the expression and interaction of proteins of interest.					
31898491	7	8	theme	proteins	1181:1188	arg1	expression					1151:1160	expression	1151:1160	expression	1151:1160	Western blot and co-immunoprecipitation analyses were performed to determine the expression and interaction of proteins of interest.					
31898491	9	9	theme	RESULTS	1281:1287	arg1	SRGN					1289:1292	RESULTS SRGN	1281:1292	RESULTS SRGN expressed by NSCLC cells	1281:1317	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	16	10	theme	cytoskeleton	2389:2400	arg1	reorganization					2402:2415	Rho-family GTPase-mediated cytoskeleton reorganization	2362:2415	Rho-family GTPase-mediated cytoskeleton reorganization	2362:2415	SRGN/CD44 interaction induces Rho-family GTPase-mediated cytoskeleton reorganization and facilitates Src-mediated focal adhesion turnover, leading to increased cell migration.					
31898491	8	11	theme	immunofluorescence	1252:1269	arg1	staining					1271:1278	immunofluorescence staining	1252:1278	immunofluorescence staining	1252:1278	Actin cytoskeleton organization was monitored by immunofluorescence staining.					
31898491	5	12	theme	glycosaminoglycan	809:825	arg1	modification					833:844	glycosaminoglycan (GAG) modification	809:844	glycosaminoglycan (GAG) modification	809:844	SRGN devoid of glycosaminoglycan (GAG) modification was produced by site-directed mutagenesis or chondroitinase treatment.					
31898491	5	13	theme	site-directed	862:874	arg1	mutagenesis					876:886	site-directed mutagenesis	862:886	site-directed mutagenesis	862:886	SRGN devoid of glycosaminoglycan (GAG) modification was produced by site-directed mutagenesis or chondroitinase treatment.					
31898491	12	14	theme	adhesion	1780:1787	arg1	turnover					1789:1796	focal adhesion turnover	1774:1796	focal adhesion turnover	1774:1796	SRGN/CD44 interaction promotes focal adhesion turnover via Src-mediated paxillin phosphorylation and disassembly of paxillin/FAK adhesion complex, facilitating cell migration.					
31898491	9	15	theme	NSCLC	1307:1311	arg1	cells					1313:1317	NSCLC cells	1307:1317	NSCLC cells	1307:1317	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	6	16	theme	disaccharide	1009:1020	arg1	compositions					1022:1033	the disaccharide compositions	1005:1033	the disaccharide compositions	1005:1033	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	2	17	theme	adenocarcinomas	546:560	arg1	prognosis					520:528	poor prognosis	515:528	poor prognosis of primary lung adenocarcinomas	515:560	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	17	18	theme	potential	2643:2651	arg1	strategy					2653:2660	a potential strategy	2641:2660	a potential strategy for cancer therapy	2641:2679	These findings suggest that targeting specific glycans in tumor microenvironment that serve as ligands for oncogenic pathways may be a potential strategy for cancer therapy.					
31898491	5	19	theme	modification	833:844	arg1	devoid					799:804	devoid	799:804	devoid	799:804	SRGN devoid of glycosaminoglycan (GAG) modification was produced by site-directed mutagenesis or chondroitinase treatment.					
31898491	2	20	theme	primary	533:539	arg1	adenocarcinomas					546:560	primary lung adenocarcinomas	533:560	primary lung adenocarcinomas	533:560	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	7	21	dep	expression	1151:1160	arg1	the					1147:1149	the	1147:1149	the	1147:1149	Western blot and co-immunoprecipitation analyses were performed to determine the expression and interaction of proteins of interest.					
31898491	9	22	theme	heparin	1484:1490	arg1	HS					1501:1502	HS	1501:1502	HS	1501:1502	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	9	22	theme	heparin	1484:1490	arg1	sulfate					1492:1498	heparin sulfate	1484:1498	heparin sulfate (HS)	1484:1503	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	15	23	theme	cell	2275:2278	arg1	migration					2280:2288	NSCLC cell migration	2269:2288	NSCLC cell migration	2269:2288	CONCLUSIONS Proteoglycan SRGN promotes NSCLC cell migration via the binding of its GAG motif to CD44.					
31898491	6	24	theme	quantitative	980:991	arg1	analysis					993:1000	quantitative analysis	980:1000	quantitative analysis of the disaccharide compositions	980:1033	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	4	25	theme	SRGN	715:718	arg1	role					707:710	the role	703:710	the role of SRGN in cell motility	703:735	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	4	26	dep	METHODS	620:626	arg1	knockdown					644:652	knockdown	644:652	knockdown	644:652	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	4	26	dep	METHODS	620:626	arg1	approaches					667:676	knockout approaches	658:676	knockout approaches	658:676	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	4	26	dep	METHODS	620:626	arg1	Overexpression					628:641	Overexpression	628:641	Overexpression	628:641	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	4	26	dep	METHODS	620:626	arg1	METHODS					620:626	METHODS	620:626	METHODS Overexpression, knockdown and knockout approaches	620:676	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	4	27	theme	cell	723:726	arg1	motility					728:735	cell motility	723:735	cell motility	723:735	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	16	28	theme	adhesion	2452:2459	arg1	turnover					2461:2468	Src-mediated focal adhesion turnover	2433:2468	Src-mediated focal adhesion turnover	2433:2468	SRGN/CD44 interaction induces Rho-family GTPase-mediated cytoskeleton reorganization and facilitates Src-mediated focal adhesion turnover, leading to increased cell migration.					
31898491	12	29	theme	focal	1774:1778	arg1	turnover					1789:1796	focal adhesion turnover	1774:1796	focal adhesion turnover	1774:1796	SRGN/CD44 interaction promotes focal adhesion turnover via Src-mediated paxillin phosphorylation and disassembly of paxillin/FAK adhesion complex, facilitating cell migration.					
31898491	13	30	theme	Src	2012:2014	arg1	activation					2016:2025	SRGN-mediated Src activation	1998:2025	SRGN-mediated Src activation	1998:2025	In support, depletion of Src activity or removal of CS-GAGs efficiently blocks SRGN-mediated Src activation and cell migration.					
31898491	1	31	theme	solid	329:333	arg1	tumors					335:340	several solid tumors	321:340	several solid tumors	321:340	BACKGROUND Serglycin (SRGN), previously recognized as an intracellular proteoglycan involved in the storage processes of secretory granules, has recently been shown to be upregulated in several solid tumors.					
31898491	6	32	theme	SRGN	1059:1062	arg1	GAGs					1064:1067	SRGN GAGs	1059:1067	SRGN GAGs	1059:1067	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	2	33	theme	increased	477:485	arg1	expression					487:496	increased expression	477:496	increased expression of SRGN	477:504	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	9	34	theme	glycosylated	1380:1391	arg1	form					1393:1396	a heavily glycosylated form	1370:1396	a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains	1370:1453	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	15	35	theme	motif	2317:2321	arg1	binding					2298:2304	the binding	2294:2304	the binding of its GAG motif to CD44	2294:2329	CONCLUSIONS Proteoglycan SRGN promotes NSCLC cell migration via the binding of its GAG motif to CD44.					
31898491	4	36	theme	Boyden	761:766	arg1	assays					786:791	Boyden chamber migration assays	761:791	Boyden chamber migration assays	761:791	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	13	37	theme	CS-GAGs	1971:1977	arg1	removal					1960:1966	removal	1960:1966	removal of CS-GAGs	1960:1977	In support, depletion of Src activity or removal of CS-GAGs efficiently blocks SRGN-mediated Src activation and cell migration.					
31898491	13	37	theme	CS-GAGs	1971:1977	arg1	depletion					1931:1939	depletion	1931:1939	depletion of Src activity	1931:1955	In support, depletion of Src activity or removal of CS-GAGs efficiently blocks SRGN-mediated Src activation and cell migration.					
31898491	4	38	theme	migration	776:784	arg1	assays					786:791	Boyden chamber migration assays	761:791	Boyden chamber migration assays	761:791	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	16	39	theme	Rho-family	2362:2371	arg1	reorganization					2402:2415	Rho-family GTPase-mediated cytoskeleton reorganization	2362:2415	Rho-family GTPase-mediated cytoskeleton reorganization	2362:2415	SRGN/CD44 interaction induces Rho-family GTPase-mediated cytoskeleton reorganization and facilitates Src-mediated focal adhesion turnover, leading to increased cell migration.					
31898491	2	40	theme	cell	391:394	arg1	cancer					401:406	non-small cell lung cancer	381:406	non-small cell lung cancer (NSCLC)	381:414	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	2	40	theme	cell	391:394	arg1	NSCLC					409:413	NSCLC	409:413	NSCLC	409:413	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	0	41	theme	glycosaminoglycans	105:122	arg1	interaction					86:96	the interaction	82:96	the interaction of its glycosaminoglycans with CD44	82:132	Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44.					
31898491	14	42	theme	cytoskeleton	2094:2105	arg1	reorganization					2107:2120	cytoskeleton reorganization	2094:2120	cytoskeleton reorganization mediated through RAC1 and CDC42 activation accompanied with increased lamellipodia and filopodia formation	2094:2227	SRGN also promotes cell migration via inducing cytoskeleton reorganization mediated through RAC1 and CDC42 activation accompanied with increased lamellipodia and filopodia formation.					
31898491	14	43	theme	filopodia	2209:2217	arg1	formation					2219:2227	increased lamellipodia and filopodia formation	2182:2227	increased lamellipodia and filopodia formation	2182:2227	SRGN also promotes cell migration via inducing cytoskeleton reorganization mediated through RAC1 and CDC42 activation accompanied with increased lamellipodia and filopodia formation.					
31898491	13	44	theme	activity	1948:1955	arg1	removal					1960:1966	removal	1960:1966	removal of CS-GAGs	1960:1977	In support, depletion of Src activity or removal of CS-GAGs efficiently blocks SRGN-mediated Src activation and cell migration.					
31898491	13	44	theme	activity	1948:1955	arg1	depletion					1931:1939	depletion	1931:1939	depletion of Src activity	1931:1955	In support, depletion of Src activity or removal of CS-GAGs efficiently blocks SRGN-mediated Src activation and cell migration.					
31898491	0	45	theme	Proteoglycan	0:11	arg1	serglycin					13:21	Proteoglycan serglycin	0:21	Proteoglycan serglycin	0:21	Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44.					
31898491	9	46	theme	sulfate	1431:1437	arg1	chains					1448:1453	mainly chondroitin sulfate (CS)-GAG chains	1412:1453	mainly chondroitin sulfate (CS)-GAG chains	1412:1453	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	17	47	theme	tumor	2566:2570	arg1	microenvironment					2572:2587	tumor microenvironment	2566:2587	tumor microenvironment that serve as ligands for oncogenic pathways	2566:2632	These findings suggest that targeting specific glycans in tumor microenvironment that serve as ligands for oncogenic pathways may be a potential strategy for cancer therapy.					
31898491	17	47	theme	tumor	2566:2570	arg1	ligands					2603:2609	ligands	2603:2609	ligands for oncogenic pathways	2603:2632	These findings suggest that targeting specific glycans in tumor microenvironment that serve as ligands for oncogenic pathways may be a potential strategy for cancer therapy.					
31898491	14	48	theme	lamellipodia	2192:2203	arg1	formation					2219:2227	increased lamellipodia and filopodia formation	2182:2227	increased lamellipodia and filopodia formation	2182:2227	SRGN also promotes cell migration via inducing cytoskeleton reorganization mediated through RAC1 and CDC42 activation accompanied with increased lamellipodia and filopodia formation.					
31898491	14	49	theme	cell	2066:2069	arg1	migration					2071:2079	cell migration	2066:2079	cell migration	2066:2079	SRGN also promotes cell migration via inducing cytoskeleton reorganization mediated through RAC1 and CDC42 activation accompanied with increased lamellipodia and filopodia formation.					
31898491	10	50	theme	structural	1538:1547	arg1	motif					1549:1553	the structural motif	1534:1553	the structural motif for SRGN binding to tumor cell surface CD44	1534:1597	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	10	50	theme	structural	1538:1547	arg1	moiety					1517:1522	The CS-GAG moiety	1506:1522	The CS-GAG moiety	1506:1522	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	10	50	theme	structural	1538:1547	arg1	migration					1617:1625	cell migration	1612:1625	cell migration	1612:1625	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	16	51	theme	Src-mediated	2433:2444	arg1	turnover					2461:2468	Src-mediated focal adhesion turnover	2433:2468	Src-mediated focal adhesion turnover	2433:2468	SRGN/CD44 interaction induces Rho-family GTPase-mediated cytoskeleton reorganization and facilitates Src-mediated focal adhesion turnover, leading to increased cell migration.					
31898491	0	52	theme	cell	42:45	arg1	migration					64:72	non-small cell lung cancer cell migration	32:72	non-small cell lung cancer cell migration	32:72	Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44.					
31898491	9	53	theme	-GAG	1443:1446	arg1	chains					1448:1453	mainly chondroitin sulfate (CS)-GAG chains	1412:1453	mainly chondroitin sulfate (CS)-GAG chains	1412:1453	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	10	54	theme	tumor	1575:1579	arg1	CD44					1594:1597	tumor cell surface CD44	1575:1597	tumor cell surface CD44	1575:1597	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	11	55	theme	cell	1727:1730	arg1	migration					1732:1740	cell migration	1727:1740	cell migration	1727:1740	SRGN devoid of CS-GAG modification fails to interact with CD44 and has lost the ability to promote cell migration.					
31898491	11	56	theme	CS-GAG	1643:1648	arg1	modification					1650:1661	CS-GAG modification	1643:1661	CS-GAG modification	1643:1661	SRGN devoid of CS-GAG modification fails to interact with CD44 and has lost the ability to promote cell migration.					
31898491	5	57	theme	devoid	799:804	arg1	SRGN					794:797	SRGN	794:797	SRGN devoid of glycosaminoglycan (GAG) modification	794:844	SRGN devoid of glycosaminoglycan (GAG) modification was produced by site-directed mutagenesis or chondroitinase treatment.					
31898491	15	58	theme	Proteoglycan	2242:2253	arg1	SRGN					2255:2258	CONCLUSIONS Proteoglycan SRGN	2230:2258	CONCLUSIONS Proteoglycan SRGN	2230:2258	CONCLUSIONS Proteoglycan SRGN promotes NSCLC cell migration via the binding of its GAG motif to CD44.					
31898491	17	59	theme	cancer	2666:2671	arg1	therapy					2673:2679	cancer therapy	2666:2679	cancer therapy	2666:2679	These findings suggest that targeting specific glycans in tumor microenvironment that serve as ligands for oncogenic pathways may be a potential strategy for cancer therapy.					
31898491	11	60	theme	devoid	1633:1638	arg1	SRGN					1628:1631	SRGN	1628:1631	SRGN devoid of CS-GAG modification	1628:1661	SRGN devoid of CS-GAG modification fails to interact with CD44 and has lost the ability to promote cell migration.					
31898491	12	61	theme	paxillin/FAK	1859:1870	arg1	complex					1881:1887	paxillin/FAK adhesion complex	1859:1887	paxillin/FAK adhesion complex	1859:1887	SRGN/CD44 interaction promotes focal adhesion turnover via Src-mediated paxillin phosphorylation and disassembly of paxillin/FAK adhesion complex, facilitating cell migration.					
31898491	9	62	theme	lesser	1465:1470	arg1	extent					1472:1477	a lesser extent	1463:1477	a lesser extent	1463:1477	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	12	63	theme	complex	1881:1887	arg1	phosphorylation					1824:1838	Src-mediated paxillin phosphorylation	1802:1838	Src-mediated paxillin phosphorylation	1802:1838	SRGN/CD44 interaction promotes focal adhesion turnover via Src-mediated paxillin phosphorylation and disassembly of paxillin/FAK adhesion complex, facilitating cell migration.					
31898491	12	63	theme	complex	1881:1887	arg1	disassembly					1844:1854	disassembly	1844:1854	disassembly of paxillin/FAK adhesion complex	1844:1887	SRGN/CD44 interaction promotes focal adhesion turnover via Src-mediated paxillin phosphorylation and disassembly of paxillin/FAK adhesion complex, facilitating cell migration.					
31898491	10	64	theme	surface	1586:1592	arg1	CD44					1594:1597	tumor cell surface CD44	1575:1597	tumor cell surface CD44	1575:1597	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	1	65	theme	secretory	256:264	arg1	granules					266:273	secretory granules	256:273	secretory granules	256:273	BACKGROUND Serglycin (SRGN), previously recognized as an intracellular proteoglycan involved in the storage processes of secretory granules, has recently been shown to be upregulated in several solid tumors.					
31898491	8	66	theme	cytoskeleton	1209:1220	arg1	organization					1222:1233	Actin cytoskeleton organization	1203:1233	Actin cytoskeleton organization	1203:1233	Actin cytoskeleton organization was monitored by immunofluorescence staining.					
31898491	6	67	theme	mass	946:949	arg1	spectrometry					951:962	Liquid chromatography/tandem mass spectrometry	917:962	Liquid chromatography/tandem mass spectrometry	917:962	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	16	68	theme	SRGN/CD44	2332:2340	arg1	interaction					2342:2352	SRGN/CD44 interaction	2332:2352	SRGN/CD44 interaction	2332:2352	SRGN/CD44 interaction induces Rho-family GTPase-mediated cytoskeleton reorganization and facilitates Src-mediated focal adhesion turnover, leading to increased cell migration.					
31898491	6	69	theme	Liquid	917:922	arg1	spectrometry					951:962	Liquid chromatography/tandem mass spectrometry	917:962	Liquid chromatography/tandem mass spectrometry	917:962	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	17	70	theme	oncogenic	2615:2623	arg1	pathways					2625:2632	oncogenic pathways	2615:2632	oncogenic pathways	2615:2632	These findings suggest that targeting specific glycans in tumor microenvironment that serve as ligands for oncogenic pathways may be a potential strategy for cancer therapy.					
31898491	10	71	theme	cell	1612:1615	arg1	motif					1549:1553	the structural motif	1534:1553	the structural motif for SRGN binding to tumor cell surface CD44	1534:1597	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	10	71	theme	cell	1612:1615	arg1	moiety					1517:1522	The CS-GAG moiety	1506:1522	The CS-GAG moiety	1506:1522	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	10	71	theme	cell	1612:1615	arg1	migration					1617:1625	cell migration	1612:1625	cell migration	1612:1625	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	0	72	theme	cancer	52:57	arg1	migration					64:72	non-small cell lung cancer cell migration	32:72	non-small cell lung cancer cell migration	32:72	Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44.					
31898491	4	73	theme	knockout	658:665	arg1	approaches					667:676	knockout approaches	658:676	knockout approaches	658:676	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	6	74	theme	compositions	1022:1033	arg1	extent					1049:1054	sulfation extent	1039:1054	sulfation extent of SRGN GAGs	1039:1067	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	6	74	theme	compositions	1022:1033	arg1	analysis					993:1000	quantitative analysis	980:1000	quantitative analysis of the disaccharide compositions	980:1033	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	12	75	theme	Src-mediated	1802:1813	arg1	phosphorylation					1824:1838	Src-mediated paxillin phosphorylation	1802:1838	Src-mediated paxillin phosphorylation	1802:1838	SRGN/CD44 interaction promotes focal adhesion turnover via Src-mediated paxillin phosphorylation and disassembly of paxillin/FAK adhesion complex, facilitating cell migration.					
31898491	13	76	theme	cell	2031:2034	arg1	migration					2036:2044	cell migration	2031:2044	cell migration	2031:2044	In support, depletion of Src activity or removal of CS-GAGs efficiently blocks SRGN-mediated Src activation and cell migration.					
31898491	1	77	theme	intracellular	192:204	arg1	proteoglycan					206:217	an intracellular proteoglycan	189:217	an intracellular proteoglycan involved in the storage processes of secretory granules	189:273	BACKGROUND Serglycin (SRGN), previously recognized as an intracellular proteoglycan involved in the storage processes of secretory granules, has recently been shown to be upregulated in several solid tumors.					
31898491	2	78	theme	lung	541:544	arg1	adenocarcinomas					546:560	primary lung adenocarcinomas	533:560	primary lung adenocarcinomas	533:560	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	15	79	theme	NSCLC	2269:2273	arg1	migration					2280:2288	NSCLC cell migration	2269:2288	NSCLC cell migration	2269:2288	CONCLUSIONS Proteoglycan SRGN promotes NSCLC cell migration via the binding of its GAG motif to CD44.					
31898491	7	80	theme	co-immunoprecipitation	1087:1108	arg1	analyses					1110:1117	Western blot and co-immunoprecipitation analyses	1070:1117	analyses	1110:1117	Western blot and co-immunoprecipitation analyses were performed to determine the expression and interaction of proteins of interest.					
31898491	3	81	theme	underlying	576:585	arg1	mechanism					587:595	the underlying mechanism	572:595	the underlying mechanism	572:595	However, the underlying mechanism remains to be defined.					
31898491	7	82	theme	Western	1070:1076	arg1	blot					1078:1081	Western blot and co-immunoprecipitation analyses	1070:1117	blot	1078:1081	Western blot and co-immunoprecipitation analyses were performed to determine the expression and interaction of proteins of interest.					
31898491	9	83	theme	extracellular	1346:1358	arg1	matrix					1360:1365	the extracellular matrix	1342:1365	the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains	1342:1453	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	1	84	theme	BACKGROUND	135:144	arg1	SRGN					157:160	SRGN	157:160	SRGN	157:160	BACKGROUND Serglycin (SRGN), previously recognized as an intracellular proteoglycan involved in the storage processes of secretory granules, has recently been shown to be upregulated in several solid tumors.					
31898491	1	84	theme	BACKGROUND	135:144	arg1	Serglycin					146:154	BACKGROUND Serglycin	135:154	BACKGROUND Serglycin (SRGN)	135:161	BACKGROUND Serglycin (SRGN), previously recognized as an intracellular proteoglycan involved in the storage processes of secretory granules, has recently been shown to be upregulated in several solid tumors.					
31898491	12	85	theme	SRGN/CD44	1743:1751	arg1	interaction					1753:1763	SRGN/CD44 interaction	1743:1763	SRGN/CD44 interaction	1743:1763	SRGN/CD44 interaction promotes focal adhesion turnover via Src-mediated paxillin phosphorylation and disassembly of paxillin/FAK adhesion complex, facilitating cell migration.					
31898491	9	86	gly	glycosylated	1380:1391	arg1	form					1393:1396	a heavily glycosylated form	1370:1396	a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains	1370:1453	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	13	87	theme	SRGN-mediated	1998:2010	arg1	activation					2016:2025	SRGN-mediated Src activation	1998:2025	SRGN-mediated Src activation	1998:2025	In support, depletion of Src activity or removal of CS-GAGs efficiently blocks SRGN-mediated Src activation and cell migration.					
31898491	16	88	theme	cell	2492:2495	arg1	migration					2497:2505	increased cell migration	2482:2505	increased cell migration	2482:2505	SRGN/CD44 interaction induces Rho-family GTPase-mediated cytoskeleton reorganization and facilitates Src-mediated focal adhesion turnover, leading to increased cell migration.					
31898491	5	89	theme	chondroitinase	891:904	arg1	treatment					906:914	chondroitinase treatment	891:914	chondroitinase treatment	891:914	SRGN devoid of glycosaminoglycan (GAG) modification was produced by site-directed mutagenesis or chondroitinase treatment.					
31898491	6	90	theme	GAGs	1064:1067	arg1	extent					1049:1054	sulfation extent	1039:1054	sulfation extent of SRGN GAGs	1039:1067	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	6	90	theme	GAGs	1064:1067	arg1	analysis					993:1000	quantitative analysis	980:1000	quantitative analysis of the disaccharide compositions	980:1033	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	16	91	theme	increased	2482:2490	arg1	migration					2497:2505	increased cell migration	2482:2505	increased cell migration	2482:2505	SRGN/CD44 interaction induces Rho-family GTPase-mediated cytoskeleton reorganization and facilitates Src-mediated focal adhesion turnover, leading to increased cell migration.					
31898491	14	92	theme	RAC1	2139:2142	arg1	activation					2154:2163	RAC1 and CDC42 activation	2139:2163	RAC1 and CDC42 activation accompanied with increased lamellipodia and filopodia formation	2139:2227	SRGN also promotes cell migration via inducing cytoskeleton reorganization mediated through RAC1 and CDC42 activation accompanied with increased lamellipodia and filopodia formation.					
31898491	15	93	theme	GAG	2313:2315	arg1	motif					2317:2321	its GAG motif	2309:2321	its GAG motif	2309:2321	CONCLUSIONS Proteoglycan SRGN promotes NSCLC cell migration via the binding of its GAG motif to CD44.					
31898491	6	94	theme	sulfation	1039:1047	arg1	extent					1049:1054	sulfation extent	1039:1054	sulfation extent of SRGN GAGs	1039:1067	Liquid chromatography/tandem mass spectrometry was applied for quantitative analysis of the disaccharide compositions and sulfation extent of SRGN GAGs.					
31898491	2	95	theme	poor	515:518	arg1	prognosis					520:528	poor prognosis	515:528	poor prognosis of primary lung adenocarcinomas	515:560	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	9	96	with	matrix	1360:1365	arg1	HS					1501:1502	HS	1501:1502	HS	1501:1502	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	9	96	with	matrix	1360:1365	arg1	sulfate					1492:1498	heparin sulfate	1484:1498	heparin sulfate (HS)	1484:1503	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	16	97	theme	GTPase-mediated	2373:2387	arg1	reorganization					2402:2415	Rho-family GTPase-mediated cytoskeleton reorganization	2362:2415	Rho-family GTPase-mediated cytoskeleton reorganization	2362:2415	SRGN/CD44 interaction induces Rho-family GTPase-mediated cytoskeleton reorganization and facilitates Src-mediated focal adhesion turnover, leading to increased cell migration.					
31898491	2	98	theme	SRGN	501:504	arg1	expression					487:496	increased expression	477:496	increased expression of SRGN	477:504	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	4	99	theme	chamber	768:774	arg1	assays					786:791	Boyden chamber migration assays	761:791	Boyden chamber migration assays	761:791	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	13	100	theme	Src	1944:1946	arg1	activity					1948:1955	Src activity	1944:1955	Src activity	1944:1955	In support, depletion of Src activity or removal of CS-GAGs efficiently blocks SRGN-mediated Src activation and cell migration.					
31898491	2	101	theme	non-small	381:389	arg1	cancer					401:406	non-small cell lung cancer	381:406	non-small cell lung cancer (NSCLC)	381:414	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	2	101	theme	non-small	381:389	arg1	NSCLC					409:413	NSCLC	409:413	NSCLC	409:413	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	9	102	theme	chondroitin	1419:1429	arg1	CS					1440:1441	CS	1440:1441	CS	1440:1441	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	9	102	theme	chondroitin	1419:1429	arg1	sulfate					1431:1437	mainly chondroitin sulfate	1412:1437	mainly chondroitin sulfate (CS)-GAG chains	1412:1453	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	8	103	theme	Actin	1203:1207	arg1	organization					1222:1233	Actin cytoskeleton organization	1203:1233	Actin cytoskeleton organization	1203:1233	Actin cytoskeleton organization was monitored by immunofluorescence staining.					
31898491	2	104	theme	malignant	425:433	arg1	phenotypes					435:444	malignant phenotypes	425:444	malignant phenotypes	425:444	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	10	105	theme	CS-GAG	1510:1515	arg1	motif					1549:1553	the structural motif	1534:1553	the structural motif for SRGN binding to tumor cell surface CD44	1534:1597	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	10	105	theme	CS-GAG	1510:1515	arg1	moiety					1517:1522	The CS-GAG moiety	1506:1522	The CS-GAG moiety	1506:1522	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	10	105	theme	CS-GAG	1510:1515	arg1	migration					1617:1625	cell migration	1612:1625	cell migration	1612:1625	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	0	106	theme	non-small	32:40	arg1	migration					64:72	non-small cell lung cancer cell migration	32:72	non-small cell lung cancer cell migration	32:72	Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44.					
31898491	14	107	theme	increased	2182:2190	arg1	formation					2219:2227	increased lamellipodia and filopodia formation	2182:2227	increased lamellipodia and filopodia formation	2182:2227	SRGN also promotes cell migration via inducing cytoskeleton reorganization mediated through RAC1 and CDC42 activation accompanied with increased lamellipodia and filopodia formation.					
31898491	4	108	theme	wound	743:747	arg1	healing					749:755	wound healing	743:755	wound healing	743:755	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
31898491	16	109	theme	focal	2446:2450	arg1	turnover					2461:2468	Src-mediated focal adhesion turnover	2433:2468	Src-mediated focal adhesion turnover	2433:2468	SRGN/CD44 interaction induces Rho-family GTPase-mediated cytoskeleton reorganization and facilitates Src-mediated focal adhesion turnover, leading to increased cell migration.					
31898491	0	110	theme	lung	47:50	arg1	migration					64:72	non-small cell lung cancer cell migration	32:72	non-small cell lung cancer cell migration	32:72	Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44.					
31898491	2	111	theme	CD44-dependent	451:464	arg1	manner					466:471	a CD44-dependent manner	449:471	a CD44-dependent manner	449:471	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	2	112	theme	lung	396:399	arg1	cancer					401:406	non-small cell lung cancer	381:406	non-small cell lung cancer (NSCLC)	381:414	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	2	112	theme	lung	396:399	arg1	NSCLC					409:413	NSCLC	409:413	NSCLC	409:413	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	0	113	theme	cell	59:62	arg1	migration					64:72	non-small cell lung cancer cell migration	32:72	non-small cell lung cancer cell migration	32:72	Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44.					
31898491	15	114	theme	CONCLUSIONS	2230:2240	arg1	SRGN					2255:2258	CONCLUSIONS Proteoglycan SRGN	2230:2258	CONCLUSIONS Proteoglycan SRGN	2230:2258	CONCLUSIONS Proteoglycan SRGN promotes NSCLC cell migration via the binding of its GAG motif to CD44.					
31898491	2	115	from	SRGN	373:376	arg1	cancer					401:406	non-small cell lung cancer	381:406	non-small cell lung cancer (NSCLC)	381:414	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	2	115	from	SRGN	373:376	arg1	NSCLC					409:413	NSCLC	409:413	NSCLC	409:413	We have previously shown that SRGN in non-small cell lung cancer (NSCLC) promotes malignant phenotypes in a CD44-dependent manner and increased expression of SRGN predicts poor prognosis of primary lung adenocarcinomas.					
31898491	14	116	theme	CDC42	2148:2152	arg1	activation					2154:2163	RAC1 and CDC42 activation	2139:2163	RAC1 and CDC42 activation accompanied with increased lamellipodia and filopodia formation	2139:2227	SRGN also promotes cell migration via inducing cytoskeleton reorganization mediated through RAC1 and CDC42 activation accompanied with increased lamellipodia and filopodia formation.					
31898491	1	117	theme	several	321:327	arg1	tumors					335:340	several solid tumors	321:340	several solid tumors	321:340	BACKGROUND Serglycin (SRGN), previously recognized as an intracellular proteoglycan involved in the storage processes of secretory granules, has recently been shown to be upregulated in several solid tumors.					
31898491	12	118	theme	cell	1903:1906	arg1	migration					1908:1916	cell migration	1903:1916	cell migration	1903:1916	SRGN/CD44 interaction promotes focal adhesion turnover via Src-mediated paxillin phosphorylation and disassembly of paxillin/FAK adhesion complex, facilitating cell migration.					
31898491	10	119	theme	SRGN	1559:1562	arg1	binding					1564:1570	SRGN binding	1559:1570	SRGN binding to tumor cell surface CD44	1559:1597	The CS-GAG moiety serves as the structural motif for SRGN binding to tumor cell surface CD44 and promotes cell migration.					
31898491	1	120	theme	storage	235:241	arg1	processes					243:251	the storage processes	231:251	the storage processes of secretory granules	231:273	BACKGROUND Serglycin (SRGN), previously recognized as an intracellular proteoglycan involved in the storage processes of secretory granules, has recently been shown to be upregulated in several solid tumors.					
31898491	9	121	from	matrix	1360:1365	arg1	form					1393:1396	a heavily glycosylated form	1370:1396	a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains	1370:1453	RESULTS SRGN expressed by NSCLC cells is readily secreted to the extracellular matrix in a heavily glycosylated form attached with mainly chondroitin sulfate (CS)-GAG chains, and to a lesser extent with heparin sulfate (HS).					
31898491	11	122	theme	modification	1650:1661	arg1	devoid					1633:1638	devoid	1633:1638	devoid	1633:1638	SRGN devoid of CS-GAG modification fails to interact with CD44 and has lost the ability to promote cell migration.					
31898491	4	123	from	role	707:710	arg1	motility					728:735	cell motility	723:735	cell motility	723:735	METHODS Overexpression, knockdown and knockout approaches were performed to assess the role of SRGN in cell motility using wound healing and Boyden chamber migration assays.					
32361021	0	0	theme	therapeutic	94:104	arg1	antibodies					117:126	therapeutic Monoclonal antibodies	94:126	therapeutic Monoclonal antibodies	94:126	Differential influence on antibody dependent cellular phagocytosis by different glycoforms on therapeutic Monoclonal antibodies.					
32361021	6	1	contain	possess	1206:1212	arg1	they					1201:1204	they	1201:1204	they	1201:1204	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	1	contain	possess	1206:1212	arg2	CDC					1259:1261	CDC	1259:1261	CDC	1259:1261	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	1	contain	possess	1206:1212	arg2	ADCC					1250:1253	ADCC	1250:1253	ADCC	1250:1253	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	1	contain	possess	1206:1212	arg2	activities					1238:1247	other effector function activities	1214:1247	other effector function activities (ADCC and CDC)	1214:1262	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	2	dep	activities	1238:1247	arg1	activities					1238:1247	other effector function activities	1214:1247	other effector function activities (ADCC and CDC)	1214:1262	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	2	dep	activities	1238:1247	arg1	CDC					1259:1261	CDC	1259:1261	CDC	1259:1261	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	2	dep	activities	1238:1247	arg1	ADCC					1250:1253	ADCC	1250:1253	ADCC	1250:1253	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	0	3	from	influence	13:21	arg1	antibodies					117:126	therapeutic Monoclonal antibodies	94:126	therapeutic Monoclonal antibodies	94:126	Differential influence on antibody dependent cellular phagocytosis by different glycoforms on therapeutic Monoclonal antibodies.					
32361021	0	3	from	influence	13:21	arg1	phagocytosis					54:65	antibody dependent cellular phagocytosis	26:65	antibody dependent cellular phagocytosis by different glycoforms	26:89	Differential influence on antibody dependent cellular phagocytosis by different glycoforms on therapeutic Monoclonal antibodies.					
32361021	6	4	theme	function	1229:1236	arg1	activities					1238:1247	other effector function activities	1214:1247	other effector function activities (ADCC and CDC)	1214:1262	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	4	theme	function	1229:1236	arg1	CDC					1259:1261	CDC	1259:1261	CDC	1259:1261	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	4	theme	function	1229:1236	arg1	ADCC					1250:1253	ADCC	1250:1253	ADCC	1250:1253	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	2	5	theme	Cellular	361:368	arg1	Phagocytosis					370:381	Antibody Dependent Cellular Phagocytosis	342:381	Antibody Dependent Cellular Phagocytosis (ADCP)	342:388	One such effector function activity is Antibody Dependent Cellular Phagocytosis (ADCP), which has been shown to be mediated primarily through the activating FcγR, FcγRIIa, on macrophages and neutrophils.					
32361021	2	5	theme	Cellular	361:368	arg1	ADCP					384:387	ADCP	384:387	ADCP	384:387	One such effector function activity is Antibody Dependent Cellular Phagocytosis (ADCP), which has been shown to be mediated primarily through the activating FcγR, FcγRIIa, on macrophages and neutrophils.					
32361021	0	6	theme	Monoclonal	106:115	arg1	antibodies					117:126	therapeutic Monoclonal antibodies	94:126	therapeutic Monoclonal antibodies	94:126	Differential influence on antibody dependent cellular phagocytosis by different glycoforms on therapeutic Monoclonal antibodies.					
32361021	2	7	theme	Dependent	351:359	arg1	Phagocytosis					370:381	Antibody Dependent Cellular Phagocytosis	342:381	Antibody Dependent Cellular Phagocytosis (ADCP)	342:388	One such effector function activity is Antibody Dependent Cellular Phagocytosis (ADCP), which has been shown to be mediated primarily through the activating FcγR, FcγRIIa, on macrophages and neutrophils.					
32361021	2	7	theme	Dependent	351:359	arg1	ADCP					384:387	ADCP	384:387	ADCP	384:387	One such effector function activity is Antibody Dependent Cellular Phagocytosis (ADCP), which has been shown to be mediated primarily through the activating FcγR, FcγRIIa, on macrophages and neutrophils.					
32361021	4	8	theme	desirable	817:825	arg1	assays					805:810	such assays	800:810	such assays less desirable	800:825	Primary cell assays for ADCP are often laborious and subject to donor to donor variability, making such assays less desirable for product characterization.					
32361021	3	9	theme	quality	520:526	arg1	impactful					557:565	impactful	557:565	impactful	557:565	The critical quality attributes that are the most impactful and predictive of ADCP activity, and therefore most suitable to monitor during IgG1 antibody manufacturing, are not well established.					
32361021	3	9	theme	quality	520:526	arg1	attributes					528:537	The critical quality attributes	507:537	The critical quality attributes	507:537	The critical quality attributes that are the most impactful and predictive of ADCP activity, and therefore most suitable to monitor during IgG1 antibody manufacturing, are not well established.					
32361021	6	10	theme	effector	1220:1227	arg1	activities					1238:1247	other effector function activities	1214:1247	other effector function activities (ADCC and CDC)	1214:1262	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	10	theme	effector	1220:1227	arg1	CDC					1259:1261	CDC	1259:1261	CDC	1259:1261	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	10	theme	effector	1220:1227	arg1	ADCC					1250:1253	ADCC	1250:1253	ADCC	1250:1253	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	5	11	theme	ADCP	888:891	arg1	assay					907:911	an ADCP reporter gene assay	885:911	an ADCP reporter gene assay	885:911	By developing and employing an ADCP reporter gene assay, we have been able to determine with high sensitivity the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies.					
32361021	5	12	theme	high	950:953	arg1	sensitivity					955:965	high sensitivity	950:965	high sensitivity	950:965	By developing and employing an ADCP reporter gene assay, we have been able to determine with high sensitivity the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies.					
32361021	1	13	theme	IgG1	191:194	arg1	subclass					196:203	the IgG1 subclass	187:203	the IgG1 subclass	187:203	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	7	14	theme	strong	1427:1432	arg1	influence					1434:1442	a surprisingly strong influence	1412:1442	a surprisingly strong influence of β-galactosylation	1412:1463	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	4	15	theme	product	831:837	arg1	characterization					839:854	product characterization	831:854	product characterization	831:854	Primary cell assays for ADCP are often laborious and subject to donor to donor variability, making such assays less desirable for product characterization.					
32361021	2	16	theme	function	321:328	arg1	activity					330:337	effector function activity	312:337	effector function activity	312:337	One such effector function activity is Antibody Dependent Cellular Phagocytosis (ADCP), which has been shown to be mediated primarily through the activating FcγR, FcγRIIa, on macrophages and neutrophils.					
32361021	1	17	theme	subclass	196:203	arg1	mAbs					164:167	mAbs	164:167	mAbs	164:167	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	1	17	theme	subclass	196:203	arg1	antibodies					152:161	Therapeutic monoclonal antibodies	129:161	Therapeutic monoclonal antibodies (mAbs)	129:168	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	0	18	theme	Differential	0:11	arg1	influence					13:21	Differential influence	0:21	Differential influence on antibody dependent cellular phagocytosis by different glycoforms on therapeutic Monoclonal antibodies	0:126	Differential influence on antibody dependent cellular phagocytosis by different glycoforms on therapeutic Monoclonal antibodies.					
32361021	5	19	theme	multiple	1034:1041	arg1	antibodies					1058:1067	multiple different IgG1 antibodies	1034:1067	multiple different IgG1 antibodies	1034:1067	By developing and employing an ADCP reporter gene assay, we have been able to determine with high sensitivity the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies.					
32361021	7	20	theme	mannose	1474:1480	arg1	levels					1482:1487	high mannose levels	1469:1487	high mannose levels	1469:1487	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	2	21	theme	effector	312:319	arg1	activity					330:337	effector function activity	312:337	effector function activity	312:337	One such effector function activity is Antibody Dependent Cellular Phagocytosis (ADCP), which has been shown to be mediated primarily through the activating FcγR, FcγRIIa, on macrophages and neutrophils.					
32361021	7	22	theme	different	1300:1308	arg1	antibodies					1315:1324	different IgG1 antibodies	1300:1324	different IgG1 antibodies	1300:1324	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	5	23	theme	reporter	893:900	arg1	assay					907:911	an ADCP reporter gene assay	885:911	an ADCP reporter gene assay	885:911	By developing and employing an ADCP reporter gene assay, we have been able to determine with high sensitivity the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies.					
32361021	5	24	theme	mediated	1013:1020	arg1	ADCP					1022:1025	FcγRIIa mediated ADCP	1005:1025	FcγRIIa mediated ADCP	1005:1025	By developing and employing an ADCP reporter gene assay, we have been able to determine with high sensitivity the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies.					
32361021	6	25	theme	other	1214:1218	arg1	activities					1238:1247	other effector function activities	1214:1247	other effector function activities (ADCC and CDC)	1214:1262	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	25	theme	other	1214:1218	arg1	CDC					1259:1261	CDC	1259:1261	CDC	1259:1261	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	25	theme	other	1214:1218	arg1	ADCC					1250:1253	ADCC	1250:1253	ADCC	1250:1253	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	1	26	theme	action	295:300	arg1	mechanism					282:290	their mechanism	276:290	their mechanism of action	276:300	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	5	27	theme	different	1043:1051	arg1	antibodies					1058:1067	multiple different IgG1 antibodies	1034:1067	multiple different IgG1 antibodies	1034:1067	By developing and employing an ADCP reporter gene assay, we have been able to determine with high sensitivity the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies.					
32361021	1	28	theme	Therapeutic	129:139	arg1	mAbs					164:167	mAbs	164:167	mAbs	164:167	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	1	28	theme	Therapeutic	129:139	arg1	antibodies					152:161	Therapeutic monoclonal antibodies	129:161	Therapeutic monoclonal antibodies (mAbs)	129:168	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	0	29	theme	cellular	45:52	arg1	phagocytosis					54:65	antibody dependent cellular phagocytosis	26:65	antibody dependent cellular phagocytosis by different glycoforms	26:89	Differential influence on antibody dependent cellular phagocytosis by different glycoforms on therapeutic Monoclonal antibodies.					
32361021	5	30	theme	IgG1	1053:1056	arg1	antibodies					1058:1067	multiple different IgG1 antibodies	1034:1067	multiple different IgG1 antibodies	1034:1067	By developing and employing an ADCP reporter gene assay, we have been able to determine with high sensitivity the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies.					
32361021	3	31	theme	ADCP	585:588	arg1	activity					590:597	ADCP activity	585:597	ADCP activity	585:597	The critical quality attributes that are the most impactful and predictive of ADCP activity, and therefore most suitable to monitor during IgG1 antibody manufacturing, are not well established.					
32361021	0	32	theme	dependent	35:43	arg1	phagocytosis					54:65	antibody dependent cellular phagocytosis	26:65	antibody dependent cellular phagocytosis by different glycoforms	26:89	Differential influence on antibody dependent cellular phagocytosis by different glycoforms on therapeutic Monoclonal antibodies.					
32361021	7	33	theme	β-galactosylation	1447:1463	arg1	influence					1434:1442	a surprisingly strong influence	1412:1442	a surprisingly strong influence of β-galactosylation	1412:1463	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	7	33	theme	β-galactosylation	1447:1463	arg1	levels					1482:1487	high mannose levels	1469:1487	high mannose levels	1469:1487	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	7	34	theme	glycan	1364:1369	arg1	species					1371:1377	glycan species	1364:1377	glycan species	1364:1377	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	3	35	theme	activity	590:597	arg1	predictive					571:580	predictive	571:580	predictive	571:580	The critical quality attributes that are the most impactful and predictive of ADCP activity, and therefore most suitable to monitor during IgG1 antibody manufacturing, are not well established.					
32361021	7	36	theme	different	1339:1347	arg1	sensitivity					1349:1359	markedly different sensitivity	1330:1359	markedly different sensitivity to glycan species	1330:1377	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	1	37	theme	effector	221:228	arg1	activities					239:248	effector function activities	221:248	effector function activities that may be important for their mechanism of action	221:300	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	1	38	theme	monoclonal	141:150	arg1	mAbs					164:167	mAbs	164:167	mAbs	164:167	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	1	38	theme	monoclonal	141:150	arg1	antibodies					152:161	Therapeutic monoclonal antibodies	129:161	Therapeutic monoclonal antibodies (mAbs)	129:168	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	5	39	theme	FcγRIIa	1005:1011	arg1	ADCP					1022:1025	FcγRIIa mediated ADCP	1005:1025	FcγRIIa mediated ADCP	1005:1025	By developing and employing an ADCP reporter gene assay, we have been able to determine with high sensitivity the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies.					
32361021	4	40	theme	such	800:803	arg1	assays					805:810	such assays	800:810	such assays less desirable	800:825	Primary cell assays for ADCP are often laborious and subject to donor to donor variability, making such assays less desirable for product characterization.					
32361021	5	41	theme	gene	902:905	arg1	assay					907:911	an ADCP reporter gene assay	885:911	an ADCP reporter gene assay	885:911	By developing and employing an ADCP reporter gene assay, we have been able to determine with high sensitivity the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies.					
32361021	7	42	theme	IgG1	1310:1313	arg1	antibodies					1315:1324	different IgG1 antibodies	1300:1324	different IgG1 antibodies	1300:1324	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	4	43	theme	Primary	701:707	arg1	assays					714:719	Primary cell assays	701:719	Primary cell assays for ADCP	701:728	Primary cell assays for ADCP are often laborious and subject to donor to donor variability, making such assays less desirable for product characterization.					
32361021	6	44	theme	IgG1	1106:1109	arg1	mediators					1138:1146	very potent mediators	1126:1146	very potent mediators of ADCP	1126:1154	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	44	theme	IgG1	1106:1109	arg1	antibodies					1111:1120	some IgG1 antibodies	1101:1120	some IgG1 antibodies	1101:1120	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	45	theme	potent	1131:1136	arg1	mediators					1138:1146	very potent mediators	1126:1146	very potent mediators of ADCP	1126:1154	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	45	theme	potent	1131:1136	arg1	antibodies					1111:1120	some IgG1 antibodies	1101:1120	some IgG1 antibodies	1101:1120	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	1	46	theme	function	230:237	arg1	activities					239:248	effector function activities	221:248	effector function activities that may be important for their mechanism of action	221:300	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	6	47	theme	ADCP	1151:1154	arg1	mediators					1138:1146	very potent mediators	1126:1146	very potent mediators of ADCP	1126:1154	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	6	47	theme	ADCP	1151:1154	arg1	antibodies					1111:1120	some IgG1 antibodies	1101:1120	some IgG1 antibodies	1101:1120	Interestingly we observed that some IgG1 antibodies are very potent mediators of ADCP while others do not mediate ADCP even though they possess other effector function activities (ADCC and CDC).					
32361021	3	48	theme	IgG1	646:649	arg1	manufacturing					660:672	IgG1 antibody manufacturing	646:672	IgG1 antibody manufacturing	646:672	The critical quality attributes that are the most impactful and predictive of ADCP activity, and therefore most suitable to monitor during IgG1 antibody manufacturing, are not well established.					
32361021	4	49	theme	cell	709:712	arg1	assays					714:719	Primary cell assays	701:719	Primary cell assays for ADCP	701:728	Primary cell assays for ADCP are often laborious and subject to donor to donor variability, making such assays less desirable for product characterization.					
32361021	1	50	theme	activities	239:248	arg1	capable					210:216	capable	210:216	capable	210:216	Therapeutic monoclonal antibodies (mAbs), particularly of the IgG1 subclass, are capable of effector function activities that may be important for their mechanism of action.					
32361021	3	51	theme	antibody	651:658	arg1	manufacturing					660:672	IgG1 antibody manufacturing	646:672	IgG1 antibody manufacturing	646:672	The critical quality attributes that are the most impactful and predictive of ADCP activity, and therefore most suitable to monitor during IgG1 antibody manufacturing, are not well established.					
32361021	5	52	theme	glycan	971:976	arg1	structures					978:987	the glycan structures	967:987	the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies	967:1067	By developing and employing an ADCP reporter gene assay, we have been able to determine with high sensitivity the glycan structures that can impact FcγRIIa mediated ADCP across multiple different IgG1 antibodies.					
32361021	7	53	contain	has	1326:1328	arg2	sensitivity					1349:1359	markedly different sensitivity	1330:1359	markedly different sensitivity to glycan species	1330:1377	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	7	53	contain	has	1326:1328	arg1	ADCP					1292:1295	ADCP	1292:1295	ADCP by different IgG1 antibodies	1292:1324	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	0	54	theme	different	70:78	arg1	glycoforms					80:89	different glycoforms	70:89	different glycoforms	70:89	Differential influence on antibody dependent cellular phagocytosis by different glycoforms on therapeutic Monoclonal antibodies.					
32361021	7	55	theme	high	1469:1472	arg1	levels					1482:1487	high mannose levels	1469:1487	high mannose levels	1469:1487	Additionally, we find that ADCP by different IgG1 antibodies has markedly different sensitivity to glycan species, with one antibody demonstrating a surprisingly strong influence of β-galactosylation and high mannose levels.					
32361021	4	56	theme	donor	774:778	arg1	variability					780:790	donor variability	774:790	donor variability	774:790	Primary cell assays for ADCP are often laborious and subject to donor to donor variability, making such assays less desirable for product characterization.					
32361021	3	57	theme	critical	511:518	arg1	impactful					557:565	impactful	557:565	impactful	557:565	The critical quality attributes that are the most impactful and predictive of ADCP activity, and therefore most suitable to monitor during IgG1 antibody manufacturing, are not well established.					
32361021	3	57	theme	critical	511:518	arg1	attributes					528:537	The critical quality attributes	507:537	The critical quality attributes	507:537	The critical quality attributes that are the most impactful and predictive of ADCP activity, and therefore most suitable to monitor during IgG1 antibody manufacturing, are not well established.					
34424752	3	0	theme	ENGase	691:696	arg1	activity					698:705	ENGase activity	691:705	ENGase activity	691:705	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	1	theme	GH18	485:488	arg1	present					510:516	present	510:516	present	510:516	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	1	theme	GH18	485:488	arg1	domain					500:505	The GH18 catalytic domain	481:505	The GH18 catalytic domain	481:505	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	6	2	theme	RNase	1246:1250	arg1	glycoprotein					1281:1292	a model high-mannose-type glycoprotein	1255:1292	a model high-mannose-type glycoprotein	1255:1292	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	2	theme	RNase	1246:1250	arg1	B					1252:1252	RNase B	1246:1252	RNase B	1246:1252	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	3	3	theme	cleaving	735:742	arg1	structure					786:794	an abundant glycan structure	767:794	an abundant glycan structure on host epithelial surfaces	767:822	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	3	theme	cleaving	735:742	arg1	glycoprotein					753:764	cleaving N-linked glycoprotein	735:764	cleaving N-linked glycoprotein	735:764	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	6	4	theme	EfEndo18A	1195:1203	arg1	capable					1219:1225	capable	1219:1225	capable	1219:1225	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	4	theme	EfEndo18A	1195:1203	arg1	EfEndo18A					1195:1203	EfEndo18A	1195:1203	EfEndo18A	1195:1203	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	4	theme	EfEndo18A	1195:1203	arg1	EndoE					1176:1180	EndoE	1176:1180	EndoE	1176:1180	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	4	theme	EfEndo18A	1195:1203	arg1	domains					1165:1171	the GH18 catalytic domains	1146:1171	the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863)	1146:1212	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	5	theme	high-mannose-type	1263:1279	arg1	glycoprotein					1281:1292	a model high-mannose-type glycoprotein	1255:1292	a model high-mannose-type glycoprotein	1255:1292	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	5	theme	high-mannose-type	1263:1279	arg1	B					1252:1252	RNase B	1246:1252	RNase B	1246:1252	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	2	6	theme	hydrolase	282:290	arg1	clusters					297:304	Three glycosyl hydrolase gene clusters	267:304	Three glycosyl hydrolase gene clusters	267:304	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	2	6	theme	hydrolase	282:290	arg1	each					307:310	each	307:310	each	307:310	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	1	7	theme	host	187:190	arg1	sites					203:207	host anatomical sites	187:207	host anatomical sites	187:207	The ability of Enterococcus faecalis to colonize host anatomical sites is dependent on its adaptive response to host conditions.					
34424752	3	8	link	N-linked	744:751	arg1	structure					786:794	an abundant glycan structure	767:794	an abundant glycan structure on host epithelial surfaces	767:822	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	8	link	N-linked	744:751	arg1	glycoprotein					753:764	cleaving N-linked glycoprotein	735:764	cleaving N-linked glycoprotein	735:764	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	10	9	theme	glycosidases	1974:1985	arg1	contribution					1958:1969	the combined contribution	1945:1969	the combined contribution of glycosidases to the virulence of E. faecalis in vivo	1945:2025	Finally, our data highlight the combined contribution of glycosidases to the virulence of E. faecalis in vivo.					
34424752	7	10	gly	deglycosylation	1377:1391	arg1	glycoproteins					1414:1426	high-mannose-type glycoproteins	1396:1426	high-mannose-type glycoproteins	1396:1426	However, it remained uncertain which glycosidase is primarily responsible for the deglycosylation of high-mannose-type glycoproteins.					
34424752	5	11	theme	glucose	1095:1101	arg1	availability					1103:1114	glucose availability	1095:1114	glucose availability	1095:1114	Additionally, we demonstrate that a constitutively active CcpA variant represses the expression of CcpA-regulated genes irrespective of glucose availability.					
34424752	3	12	located	present	510:516	arg2	domain					500:505	The GH18 catalytic domain	481:505	The GH18 catalytic domain	481:505	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	12	located	present	510:516	arg1	proteins					521:528	proteins	521:528	proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity	521:684	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	12	located	present	510:516	arg2	present					510:516	present	510:516	present	510:516	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	12	located	present	510:516	arg1	chitinases					560:569	chitinases	560:569	chitinases	560:569	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	12	located	present	510:516	arg1	endo-β-N-acetylglucosaminidases					580:610	β-1,4 endo-β-N-acetylglucosaminidases	574:610	β-1,4 endo-β-N-acetylglucosaminidases (ENGases)	574:620	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	9	13	theme	glycoprotein	1786:1797	arg1	representative					1753:1766	representative	1753:1766	representative	1753:1766	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	3	14	theme	β-1,4	574:578	arg1	proteins					521:528	proteins	521:528	proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity	521:684	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	14	theme	β-1,4	574:578	arg1	ENGases					613:619	ENGases	613:619	ENGases	613:619	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	14	theme	β-1,4	574:578	arg1	endo-β-N-acetylglucosaminidases					580:610	β-1,4 endo-β-N-acetylglucosaminidases	574:610	β-1,4 endo-β-N-acetylglucosaminidases (ENGases)	574:620	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	7	15	theme	glycoproteins	1414:1426	arg1	deglycosylation					1377:1391	the deglycosylation	1373:1391	the deglycosylation of high-mannose-type glycoproteins	1373:1426	However, it remained uncertain which glycosidase is primarily responsible for the deglycosylation of high-mannose-type glycoproteins.					
34424752	3	16	theme	glycan	779:784	arg1	structure					786:794	an abundant glycan structure	767:794	an abundant glycan structure on host epithelial surfaces	767:822	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	16	theme	glycan	779:784	arg1	glycoprotein					753:764	cleaving N-linked glycoprotein	735:764	cleaving N-linked glycoprotein	735:764	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	5	17	theme	CcpA-regulated	1058:1071	arg1	genes					1073:1077	CcpA-regulated genes	1058:1077	CcpA-regulated genes irrespective of glucose availability	1058:1114	Additionally, we demonstrate that a constitutively active CcpA variant represses the expression of CcpA-regulated genes irrespective of glucose availability.					
34424752	6	18	gly	glycoprotein	1281:1292	arg1	glycoprotein					1281:1292	a model high-mannose-type glycoprotein	1255:1292	a model high-mannose-type glycoprotein	1255:1292	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	18	gly	glycoprotein	1281:1292	arg1	B					1252:1252	RNase B	1246:1252	RNase B	1246:1252	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	4	19	theme	catabolite	930:939	arg1	A					949:949	carbon catabolite protein A	923:949	the transcriptional regulator carbon catabolite protein A (CcpA)	893:956	Here, we show that all three hydrolases are negatively regulated by the transcriptional regulator carbon catabolite protein A (CcpA).					
34424752	4	19	theme	catabolite	930:939	arg1	CcpA					952:955	CcpA	952:955	CcpA	952:955	Here, we show that all three hydrolases are negatively regulated by the transcriptional regulator carbon catabolite protein A (CcpA).					
34424752	5	20	theme	irrespective	1079:1090	arg1	genes					1073:1077	CcpA-regulated genes	1058:1077	CcpA-regulated genes irrespective of glucose availability	1058:1114	Additionally, we demonstrate that a constitutively active CcpA variant represses the expression of CcpA-regulated genes irrespective of glucose availability.					
34424752	8	21	theme	recombinant	1513:1523	arg1	expression					1533:1542	recombinant protein expression	1513:1542	recombinant protein expression	1513:1542	In this study, we show by mutation analysis as well as a dose-dependent analysis of recombinant protein expression that EfEndo18A is primarily responsible for deglycosylating high-mannose glycoproteins and that the glycans removed by EfEndo18A support growth under nutrient-limiting conditions in vitro.					
34424752	3	22	theme	epithelial	804:813	arg1	surfaces					815:822	host epithelial surfaces	799:822	host epithelial surfaces	799:822	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	1	23	theme	Enterococcus	153:164	arg1	faecalis					166:173	Enterococcus faecalis	153:173	Enterococcus faecalis	153:173	The ability of Enterococcus faecalis to colonize host anatomical sites is dependent on its adaptive response to host conditions.					
34424752	2	24	theme	glucose-limiting	452:467	arg1	conditions					469:478	glucose-limiting conditions	452:478	glucose-limiting conditions	452:478	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	8	25	theme	expression	1533:1542	arg1	analysis					1464:1471	mutation analysis	1455:1471	mutation analysis as well as a dose-dependent analysis of recombinant protein expression	1455:1542	In this study, we show by mutation analysis as well as a dose-dependent analysis of recombinant protein expression that EfEndo18A is primarily responsible for deglycosylating high-mannose glycoproteins and that the glycans removed by EfEndo18A support growth under nutrient-limiting conditions in vitro.					
34424752	8	25	theme	expression	1533:1542	arg1	analysis					1501:1508	a dose-dependent analysis	1484:1508	mutation analysis as well as a dose-dependent analysis of recombinant protein expression	1455:1542	In this study, we show by mutation analysis as well as a dose-dependent analysis of recombinant protein expression that EfEndo18A is primarily responsible for deglycosylating high-mannose glycoproteins and that the glycans removed by EfEndo18A support growth under nutrient-limiting conditions in vitro.					
34424752	3	26	from	present	510:516	arg1	proteins					521:528	proteins	521:528	proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity	521:684	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	26	from	present	510:516	arg1	chitinases					560:569	chitinases	560:569	chitinases	560:569	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	26	from	present	510:516	arg1	endo-β-N-acetylglucosaminidases					580:610	β-1,4 endo-β-N-acetylglucosaminidases	574:610	β-1,4 endo-β-N-acetylglucosaminidases (ENGases)	574:620	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	0	27	theme	Nutrient	79:86	arg1	Acquisition					88:98	Nutrient Acquisition	79:98	Nutrient Acquisition	79:98	Activity of CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis.					
34424752	9	28	theme	EndoE	1843:1847	arg1	domain					1833:1838	the GH18 domain	1824:1838	the GH18 domain of EndoE	1824:1847	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	9	28	theme	EndoE	1843:1847	arg1	EndoE					1843:1847	EndoE	1843:1847	EndoE	1843:1847	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	9	28	theme	EndoE	1843:1847	arg1	responsible					1862:1872	responsible	1862:1872	responsible	1862:1872	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	3	29	from	proteins	521:528	arg1	present					510:516	present	510:516	present	510:516	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	29	from	proteins	521:528	arg1	domain					500:505	The GH18 catalytic domain	481:505	The GH18 catalytic domain	481:505	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	8	30	gly	glycoproteins	1617:1629	arg1	glycoproteins					1617:1629	high-mannose glycoproteins	1604:1629	high-mannose glycoproteins	1604:1629	In this study, we show by mutation analysis as well as a dose-dependent analysis of recombinant protein expression that EfEndo18A is primarily responsible for deglycosylating high-mannose glycoproteins and that the glycans removed by EfEndo18A support growth under nutrient-limiting conditions in vitro.					
34424752	10	31	dep	in	2019:2020	arg1	vivo					2022:2025	vivo	2022:2025	vivo	2022:2025	Finally, our data highlight the combined contribution of glycosidases to the virulence of E. faecalis in vivo.					
34424752	3	32	gly	glycoprotein	753:764	arg1	structure					786:794	an abundant glycan structure	767:794	an abundant glycan structure on host epithelial surfaces	767:822	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	32	gly	glycoprotein	753:764	arg1	glycoprotein					753:764	cleaving N-linked glycoprotein	735:764	cleaving N-linked glycoprotein	735:764	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	2	33	dep	family	344:349	arg1	ef0361					370:375	ef0361	370:375	ef0361	370:375	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	2	33	dep	family	344:349	arg1	ef2863					382:387	ef2863	382:387	ef2863	382:387	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	2	33	dep	family	344:349	arg1	ef0114					362:367	ef0114	362:367	ef0114	362:367	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	8	34	theme	high-mannose	1604:1615	arg1	glycoproteins					1617:1629	high-mannose glycoproteins	1604:1629	high-mannose glycoproteins	1604:1629	In this study, we show by mutation analysis as well as a dose-dependent analysis of recombinant protein expression that EfEndo18A is primarily responsible for deglycosylating high-mannose glycoproteins and that the glycans removed by EfEndo18A support growth under nutrient-limiting conditions in vitro.					
34424752	0	35	theme	CcpA-Regulated	12:25	arg1	Hydrolases					48:57	CcpA-Regulated GH18 Family Glycosyl Hydrolases	12:57	CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis	12:135	Activity of CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis.					
34424752	3	36	attach	present	510:516	arg2	domain					500:505	The GH18 catalytic domain	481:505	The GH18 catalytic domain	481:505	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	36	attach	present	510:516	arg1	proteins					521:528	proteins	521:528	proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity	521:684	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	36	attach	present	510:516	arg2	present					510:516	present	510:516	present	510:516	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	36	attach	present	510:516	arg1	chitinases					560:569	chitinases	560:569	chitinases	560:569	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	36	attach	present	510:516	arg1	endo-β-N-acetylglucosaminidases					580:610	β-1,4 endo-β-N-acetylglucosaminidases	574:610	β-1,4 endo-β-N-acetylglucosaminidases (ENGases)	574:620	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	37	theme	β-1,4	637:641	arg1	activity					677:684	their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity	631:684	their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity	631:684	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	0	38	theme	Family	32:37	arg1	Hydrolases					48:57	CcpA-Regulated GH18 Family Glycosyl Hydrolases	12:57	CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis	12:135	Activity of CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis.					
34424752	9	39	theme	decoration	1905:1914	arg1	removal					1882:1888	the removal	1878:1888	the removal of this glycan decoration	1878:1914	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	0	40	theme	Hydrolases	48:57	arg1	Activity					0:7	Activity	0:7	Activity of CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis.	0:136	Activity of CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis.					
34424752	10	41	theme	combined	1949:1956	arg1	contribution					1958:1969	the combined contribution	1945:1969	the combined contribution of glycosidases to the virulence of E. faecalis in vivo	1945:2025	Finally, our data highlight the combined contribution of glycosidases to the virulence of E. faecalis in vivo.					
34424752	8	42	theme	mutation	1455:1462	arg1	analysis					1464:1471	mutation analysis	1455:1471	mutation analysis as well as a dose-dependent analysis of recombinant protein expression	1455:1542	In this study, we show by mutation analysis as well as a dose-dependent analysis of recombinant protein expression that EfEndo18A is primarily responsible for deglycosylating high-mannose glycoproteins and that the glycans removed by EfEndo18A support growth under nutrient-limiting conditions in vitro.					
34424752	1	43	theme	adaptive	229:236	arg1	response					238:245	its adaptive response	225:245	its adaptive response to host conditions	225:264	The ability of Enterococcus faecalis to colonize host anatomical sites is dependent on its adaptive response to host conditions.					
34424752	5	44	theme	active	1010:1015	arg1	variant					1022:1028	a constitutively active CcpA variant	993:1028	a constitutively active CcpA variant	993:1028	Additionally, we demonstrate that a constitutively active CcpA variant represses the expression of CcpA-regulated genes irrespective of glucose availability.					
34424752	3	45	theme	catalytic	490:498	arg1	present					510:516	present	510:516	present	510:516	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	45	theme	catalytic	490:498	arg1	domain					500:505	The GH18 catalytic domain	481:505	The GH18 catalytic domain	481:505	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	8	46	theme	nutrient-limiting	1694:1710	arg1	conditions					1712:1721	nutrient-limiting conditions	1694:1721	nutrient-limiting conditions in vitro	1694:1730	In this study, we show by mutation analysis as well as a dose-dependent analysis of recombinant protein expression that EfEndo18A is primarily responsible for deglycosylating high-mannose glycoproteins and that the glycans removed by EfEndo18A support growth under nutrient-limiting conditions in vitro.					
34424752	3	47	theme	N-linked	744:751	arg1	structure					786:794	an abundant glycan structure	767:794	an abundant glycan structure on host epithelial surfaces	767:822	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	47	theme	N-linked	744:751	arg1	glycoprotein					753:764	cleaving N-linked glycoprotein	735:764	cleaving N-linked glycoprotein	735:764	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	2	48	theme	gene	292:295	arg1	clusters					297:304	Three glycosyl hydrolase gene clusters	267:304	Three glycosyl hydrolase gene clusters	267:304	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	2	48	theme	gene	292:295	arg1	each					307:310	each	307:310	each	307:310	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	6	49	theme	model	1257:1261	arg1	glycoprotein					1281:1292	a model high-mannose-type glycoprotein	1255:1292	a model high-mannose-type glycoprotein	1255:1292	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	49	theme	model	1257:1261	arg1	B					1252:1252	RNase B	1246:1252	RNase B	1246:1252	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	2	50	theme	glycosyl	325:332	arg1	family					344:349	glycosyl hydrolase family 18	325:352	glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863)	325:388	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	2	50	theme	glycosyl	325:332	arg1	GH18					355:358	GH18	355:358	GH18	355:358	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	9	51	gly	glycoprotein	1786:1797	arg1	glycoprotein					1786:1797	a complex-type glycoprotein	1771:1797	a complex-type glycoprotein	1771:1797	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	1	52	theme	anatomical	192:201	arg1	sites					203:207	host anatomical sites	187:207	host anatomical sites	187:207	The ability of Enterococcus faecalis to colonize host anatomical sites is dependent on its adaptive response to host conditions.					
34424752	9	53	from	representative	1753:1766	arg1	contrast					1736:1743	contrast	1736:1743	contrast	1736:1743	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	3	54	theme	abundant	770:777	arg1	structure					786:794	an abundant glycan structure	767:794	an abundant glycan structure on host epithelial surfaces	767:822	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	3	54	theme	abundant	770:777	arg1	glycoprotein					753:764	cleaving N-linked glycoprotein	735:764	cleaving N-linked glycoprotein	735:764	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	4	55	theme	protein	941:947	arg1	A					949:949	carbon catabolite protein A	923:949	the transcriptional regulator carbon catabolite protein A (CcpA)	893:956	Here, we show that all three hydrolases are negatively regulated by the transcriptional regulator carbon catabolite protein A (CcpA).					
34424752	4	55	theme	protein	941:947	arg1	CcpA					952:955	CcpA	952:955	CcpA	952:955	Here, we show that all three hydrolases are negatively regulated by the transcriptional regulator carbon catabolite protein A (CcpA).					
34424752	6	56	theme	EndoE	1176:1180	arg1	capable					1219:1225	capable	1219:1225	capable	1219:1225	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	56	theme	EndoE	1176:1180	arg1	EfEndo18A					1195:1203	EfEndo18A	1195:1203	EfEndo18A	1195:1203	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	56	theme	EndoE	1176:1180	arg1	EndoE					1176:1180	EndoE	1176:1180	EndoE	1176:1180	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	56	theme	EndoE	1176:1180	arg1	domains					1165:1171	the GH18 catalytic domains	1146:1171	the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863)	1146:1212	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	10	57	theme	in	2019:2020	arg1	virulence					1994:2002	the virulence	1990:2002	the virulence of E. faecalis in vivo	1990:2025	Finally, our data highlight the combined contribution of glycosidases to the virulence of E. faecalis in vivo.					
34424752	5	58	theme	availability	1103:1114	arg1	irrespective					1079:1090	irrespective	1079:1090	irrespective	1079:1090	Additionally, we demonstrate that a constitutively active CcpA variant represses the expression of CcpA-regulated genes irrespective of glucose availability.					
34424752	9	59	from	contrast	1736:1743	arg1	representative					1753:1766	representative	1753:1766	representative	1753:1766	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	8	60	theme	dose-dependent	1486:1499	arg1	analysis					1501:1508	a dose-dependent analysis	1484:1508	mutation analysis as well as a dose-dependent analysis of recombinant protein expression	1455:1542	In this study, we show by mutation analysis as well as a dose-dependent analysis of recombinant protein expression that EfEndo18A is primarily responsible for deglycosylating high-mannose glycoproteins and that the glycans removed by EfEndo18A support growth under nutrient-limiting conditions in vitro.					
34424752	3	61	theme	host	799:802	arg1	surfaces					815:822	host epithelial surfaces	799:822	host epithelial surfaces	799:822	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	7	62	gly	glycoproteins	1414:1426	arg1	glycoproteins					1414:1426	high-mannose-type glycoproteins	1396:1426	high-mannose-type glycoproteins	1396:1426	However, it remained uncertain which glycosidase is primarily responsible for the deglycosylation of high-mannose-type glycoproteins.					
34424752	4	63	theme	transcriptional	897:911	arg1	regulator					913:921	the transcriptional regulator carbon catabolite protein A (CcpA)	893:956	the transcriptional regulator carbon catabolite protein A (CcpA)	893:956	Here, we show that all three hydrolases are negatively regulated by the transcriptional regulator carbon catabolite protein A (CcpA).					
34424752	8	64	from	conditions	1712:1721	arg1	vitro					1726:1730	vitro	1726:1730	vitro	1726:1730	In this study, we show by mutation analysis as well as a dose-dependent analysis of recombinant protein expression that EfEndo18A is primarily responsible for deglycosylating high-mannose glycoproteins and that the glycans removed by EfEndo18A support growth under nutrient-limiting conditions in vitro.					
34424752	5	65	theme	genes	1073:1077	arg1	expression					1044:1053	the expression	1040:1053	the expression of CcpA-regulated genes irrespective of glucose availability	1040:1114	Additionally, we demonstrate that a constitutively active CcpA variant represses the expression of CcpA-regulated genes irrespective of glucose availability.					
34424752	2	66	theme	glycosyl	273:280	arg1	clusters					297:304	Three glycosyl hydrolase gene clusters	267:304	Three glycosyl hydrolase gene clusters	267:304	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	2	66	theme	glycosyl	273:280	arg1	each					307:310	each	307:310	each	307:310	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	4	67	theme	carbon	923:928	arg1	A					949:949	carbon catabolite protein A	923:949	the transcriptional regulator carbon catabolite protein A (CcpA)	893:956	Here, we show that all three hydrolases are negatively regulated by the transcriptional regulator carbon catabolite protein A (CcpA).					
34424752	4	67	theme	carbon	923:928	arg1	CcpA					952:955	CcpA	952:955	CcpA	952:955	Here, we show that all three hydrolases are negatively regulated by the transcriptional regulator carbon catabolite protein A (CcpA).					
34424752	8	68	theme	protein	1525:1531	arg1	expression					1533:1542	recombinant protein expression	1513:1542	recombinant protein expression	1513:1542	In this study, we show by mutation analysis as well as a dose-dependent analysis of recombinant protein expression that EfEndo18A is primarily responsible for deglycosylating high-mannose glycoproteins and that the glycans removed by EfEndo18A support growth under nutrient-limiting conditions in vitro.					
34424752	7	69	theme	high-mannose-type	1396:1412	arg1	glycoproteins					1414:1426	high-mannose-type glycoproteins	1396:1426	high-mannose-type glycoproteins	1396:1426	However, it remained uncertain which glycosidase is primarily responsible for the deglycosylation of high-mannose-type glycoproteins.					
34424752	2	70	from	clusters	297:304	arg1	faecalis					397:404	E. faecalis	394:404	E. faecalis	394:404	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	9	71	theme	GH18	1828:1831	arg1	domain					1833:1838	the GH18 domain	1824:1838	the GH18 domain of EndoE	1824:1847	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	9	71	theme	GH18	1828:1831	arg1	EndoE					1843:1847	EndoE	1843:1847	EndoE	1843:1847	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	9	71	theme	GH18	1828:1831	arg1	responsible					1862:1872	responsible	1862:1872	responsible	1862:1872	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	10	72	theme	faecalis	2010:2017	arg1	virulence					1994:2002	the virulence	1990:2002	the virulence of E. faecalis in vivo	1990:2025	Finally, our data highlight the combined contribution of glycosidases to the virulence of E. faecalis in vivo.					
34424752	4	73	dep	regulator	913:921	arg1	A					949:949	carbon catabolite protein A	923:949	the transcriptional regulator carbon catabolite protein A (CcpA)	893:956	Here, we show that all three hydrolases are negatively regulated by the transcriptional regulator carbon catabolite protein A (CcpA).					
34424752	4	73	dep	regulator	913:921	arg1	CcpA					952:955	CcpA	952:955	CcpA	952:955	Here, we show that all three hydrolases are negatively regulated by the transcriptional regulator carbon catabolite protein A (CcpA).					
34424752	1	74	theme	faecalis	166:173	arg1	dependent					212:220	dependent	212:220	dependent	212:220	The ability of Enterococcus faecalis to colonize host anatomical sites is dependent on its adaptive response to host conditions.					
34424752	1	74	theme	faecalis	166:173	arg1	ability					142:148	The ability	138:148	The ability of Enterococcus faecalis to colonize host anatomical sites	138:207	The ability of Enterococcus faecalis to colonize host anatomical sites is dependent on its adaptive response to host conditions.					
34424752	9	75	theme	complex-type	1773:1784	arg1	glycoprotein					1786:1797	a complex-type glycoprotein	1771:1797	a complex-type glycoprotein	1771:1797	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	3	76	from	structure	786:794	arg1	surfaces					815:822	host epithelial surfaces	799:822	host epithelial surfaces	799:822	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	0	77	theme	Enterococcus	115:126	arg1	faecalis					128:135	Enterococcus faecalis	115:135	Enterococcus faecalis	115:135	Activity of CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis.					
34424752	6	78	theme	Previous	1117:1124	arg1	studies					1126:1132	Previous studies	1117:1132	Previous studies	1117:1132	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	0	79	theme	GH18	27:30	arg1	Hydrolases					48:57	CcpA-Regulated GH18 Family Glycosyl Hydrolases	12:57	CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis	12:135	Activity of CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis.					
34424752	0	80	theme	Glycosyl	39:46	arg1	Hydrolases					48:57	CcpA-Regulated GH18 Family Glycosyl Hydrolases	12:57	CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis	12:135	Activity of CcpA-Regulated GH18 Family Glycosyl Hydrolases That Contributes to Nutrient Acquisition and Fitness in Enterococcus faecalis.					
34424752	9	81	theme	glycan	1898:1903	arg1	decoration					1905:1914	this glycan decoration	1893:1914	this glycan decoration	1893:1914	In contrast, IgG is representative of a complex-type glycoprotein, and we demonstrate that the GH18 domain of EndoE is primarily responsible for the removal of this glycan decoration.					
34424752	6	82	theme	catalytic	1155:1163	arg1	capable					1219:1225	capable	1219:1225	capable	1219:1225	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	82	theme	catalytic	1155:1163	arg1	EfEndo18A					1195:1203	EfEndo18A	1195:1203	EfEndo18A	1195:1203	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	82	theme	catalytic	1155:1163	arg1	EndoE					1176:1180	EndoE	1176:1180	EndoE	1176:1180	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	6	82	theme	catalytic	1155:1163	arg1	domains					1165:1171	the GH18 catalytic domains	1146:1171	the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863)	1146:1212	Previous studies showed that the GH18 catalytic domains of EndoE (EF0114) and EfEndo18A (EF2863) were capable of deglycosylating RNase B, a model high-mannose-type glycoprotein.					
34424752	3	83	theme	endo-N-acetyl-β-d-glucosaminidase	643:675	arg1	activity					677:684	their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity	631:684	their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity	631:684	The GH18 catalytic domain is present in proteins that are classified as either chitinases or β-1,4 endo-β-N-acetylglucosaminidases (ENGases) based on their β-1,4 endo-N-acetyl-β-d-glucosaminidase activity, and ENGase activity is commonly associated with cleaving N-linked glycoprotein, an abundant glycan structure on host epithelial surfaces.					
34424752	2	84	theme	hydrolase	334:342	arg1	family					344:349	glycosyl hydrolase family 18	325:352	glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863)	325:388	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	2	84	theme	hydrolase	334:342	arg1	GH18					355:358	GH18	355:358	GH18	355:358	Three glycosyl hydrolase gene clusters, each belonging to glycosyl hydrolase family 18 (GH18) (ef0114, ef0361, and ef2863), in E. faecalis were previously found to be upregulated under glucose-limiting conditions.					
34424752	5	85	theme	CcpA	1017:1020	arg1	variant					1022:1028	a constitutively active CcpA variant	993:1028	a constitutively active CcpA variant	993:1028	Additionally, we demonstrate that a constitutively active CcpA variant represses the expression of CcpA-regulated genes irrespective of glucose availability.					
34424752	1	86	theme	host	250:253	arg1	conditions					255:264	host conditions	250:264	host conditions	250:264	The ability of Enterococcus faecalis to colonize host anatomical sites is dependent on its adaptive response to host conditions.					
32309832	11	0	theme	GAGs	1744:1747	arg1	removal					1733:1739	removal	1733:1739	removal of GAGs	1733:1747	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	12	1	theme	&	1903:1903	arg1	protocol					1915:1922	a high-throughput & efficient protocol	1885:1922	a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513)	1885:2049	Overall, we demonstrate a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513).					
32309832	3	2	theme	glycans	411:417	arg1	analysis					399:406	analysis	399:406	analysis of glycans along with proteins	399:437	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	8	3	theme	sample	1188:1193	arg1	in-solution					1226:1236	a filter-aided sample preparation (FASP) type serial in-solution	1173:1236	a filter-aided sample preparation (FASP) type serial in-solution digestion	1173:1246	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	12	4	theme	spectrometry-based	1933:1950	arg1	glycomic					1952:1959	mass spectrometry-based glycomic	1928:1959	mass spectrometry-based glycomic	1928:1959	Overall, we demonstrate a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513).					
32309832	11	5	theme	available	1681:1689	arg1	methods					1720:1726	available parallel (multi-pot) release methods	1681:1726	available parallel (multi-pot) release methods	1681:1726	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	6	6	theme	growing	913:919	arg1	need					921:924	a growing need	911:924	a growing need for high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture	911:1054	Thus, there is a growing need for high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture.					
32309832	5	7	dep	structures	732:741	arg1	called					743:748	called	743:748	called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs)	743:843	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	4	8	from	"	611:611	arg1	neuroscience					616:627	neuroscience	616:627	neuroscience	616:627	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	8	9	theme	serial	1219:1224	arg1	in-solution					1226:1236	a filter-aided sample preparation (FASP) type serial in-solution	1173:1236	a filter-aided sample preparation (FASP) type serial in-solution digestion	1173:1246	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	2	10	theme	post-translational	234:251	arg1	PTMs					268:271	PTMs	268:271	PTMs	268:271	However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation.					
32309832	2	10	theme	post-translational	234:251	arg1	modifications					253:265	protein post-translational modifications	226:265	protein post-translational modifications (PTMs)	226:272	However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation.					
32309832	5	11	theme	perineuronal	820:831	arg1	PNNs					839:842	PNNs	839:842	PNNs	839:842	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	5	11	theme	perineuronal	820:831	arg1	nets					833:836	perineuronal nets	820:836	perineuronal nets (PNNs)	820:843	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	4	12	gly	glycosylated	655:666	arg1	proteins					668:675	highly glycosylated proteins	648:675	highly glycosylated proteins	648:675	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	8	13	theme	GAG	1251:1253	arg1	sulfate					1303:1309	chondroitin sulfate	1291:1309	chondroitin sulfate (CS)	1291:1314	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	8	13	theme	GAG	1251:1253	arg1	sulfate					1329:1335	heparan sulfate	1321:1335	heparan sulfate (HS)	1321:1340	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	8	13	theme	GAG	1251:1253	arg1	classes					1255:1261	GAG classes	1251:1261	GAG classes	1251:1261	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	8	13	theme	GAG	1251:1253	arg1	hyaluronan					1274:1283	hyaluronan	1274:1283	hyaluronan (HA)	1274:1288	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	11	14	with	proteins	1787:1794	arg1	peptide-coverage					1808:1823	higher peptide-coverage	1801:1823	higher peptide-coverage	1801:1823	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	4	15	theme	glycosylated	655:666	arg1	proteins					668:675	highly glycosylated proteins	648:675	highly glycosylated proteins	648:675	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	5	16	theme	glycan	725:730	arg1	structures					732:741	large glycan structures	719:741	large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases	719:893	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	0	17	theme	digestion	19:27	arg1	protocol					29:36	Serial in-solution digestion protocol	0:36	Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.	0:99	Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.					
32309832	1	18	theme	proteomics	166:175	arg1	field					157:161	the field	153:161	the field of proteomics	153:175	Advancement in mass spectrometry has revolutionized the field of proteomics.					
32309832	12	19	theme	high-throughput	1887:1901	arg1	protocol					1915:1922	a high-throughput & efficient protocol	1885:1922	a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513)	1885:2049	Overall, we demonstrate a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513).					
32309832	3	20	theme	common	331:336	arg1	form					338:341	the most common form	322:341	the most common form of PTM	322:348	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	3	20	theme	common	331:336	arg1	Glycosylation					307:319	Glycosylation	307:319	Glycosylation	307:319	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	10	21	attach	isolate	1571:1577	arg2	protocol					1487:1494	This protocol	1482:1494	This protocol	1482:1494	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	10	21	attach	isolate	1571:1577	arg2	way					1527:1529	an efficient and economical way	1499:1529	an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample	1499:1631	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	10	21	attach	isolate	1571:1577	arg1	sample					1626:1631	the same sample	1617:1631	the same sample	1617:1631	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	9	22	theme	liquid	1423:1428	arg1	spectrometry					1457:1468	liquid chromatography-tandem mass spectrometry	1423:1468	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1423:1479	The GAGs and peptides are then cleaned and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32309832	9	22	theme	liquid	1423:1428	arg1	LC-MS/MS					1471:1478	LC-MS/MS	1471:1478	LC-MS/MS	1471:1478	The GAGs and peptides are then cleaned and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32309832	4	23	theme	particular	509:518	arg1	matrix					556:561	the brain extracellular matrix	532:561	the brain extracellular matrix (ECM)	532:567	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	4	23	theme	particular	509:518	arg1	interest					520:527	Of particular interest	506:527	Of particular interest	506:527	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	7	24	theme	biomolecules	1152:1163	arg1	classes					1141:1147	multiple classes	1132:1147	multiple classes of biomolecules	1132:1163	The protocol presented here integrates glycomics and proteomics to analyze multiple classes of biomolecules.					
32309832	9	25	theme	mass	1452:1455	arg1	spectrometry					1457:1468	liquid chromatography-tandem mass spectrometry	1423:1468	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1423:1479	The GAGs and peptides are then cleaned and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32309832	9	25	theme	mass	1452:1455	arg1	LC-MS/MS					1471:1478	LC-MS/MS	1471:1478	LC-MS/MS	1471:1478	The GAGs and peptides are then cleaned and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32309832	10	26	theme	cell	1555:1558	arg1	lysates					1560:1566	processing tissue or cell lysates	1534:1566	lysates	1560:1566	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	0	27	theme	proteomics	80:89	arg1	analysis					91:98	proteomics analysis	80:98	proteomics analysis	80:98	Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.					
32309832	10	28	theme	tissue	1545:1550	arg1	protocol					1487:1494	This protocol	1482:1494	This protocol	1482:1494	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	10	28	theme	tissue	1545:1550	arg1	way					1527:1529	an efficient and economical way	1499:1529	an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample	1499:1631	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	0	29	theme	Serial	0:5	arg1	protocol					29:36	Serial in-solution digestion protocol	0:36	Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.	0:99	Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.					
32309832	6	30	theme	proteomics	992:1001	arg1	analysis					1004:1011	PGs (proteomics) analysis	987:1011	PGs (proteomics) analysis	987:1011	Thus, there is a growing need for high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture.					
32309832	3	31	theme	most	366:369	arg1	processes					382:390	most biological processes	366:390	most biological processes	366:390	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	10	32	theme	various	1579:1585	arg1	classes					1591:1597	various GAG classes	1579:1597	various GAG classes	1579:1597	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	5	33	theme	ECM	794:796	arg1	components					798:807	crucial ECM components	786:807	crucial ECM components	786:807	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	5	33	theme	ECM	794:796	arg1	nets					833:836	perineuronal nets	820:836	perineuronal nets (PNNs)	820:843	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	6	34	theme	PGs	987:989	arg1	analysis					1004:1011	PGs (proteomics) analysis	987:1011	PGs (proteomics) analysis	987:1011	Thus, there is a growing need for high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture.					
32309832	3	35	dep	along	419:423	arg1	with					425:428	with	425:428	with	425:428	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	8	36	theme	heparan	1321:1327	arg1	HS					1338:1339	HS	1338:1339	HS	1338:1339	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	8	36	theme	heparan	1321:1327	arg1	sulfate					1329:1335	heparan sulfate	1321:1335	heparan sulfate (HS)	1321:1340	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	3	37	theme	relevant	486:493	arg1	questions					495:503	important biologically relevant questions	463:503	important biologically relevant questions	463:503	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	0	38	theme	spectrometry-based	47:64	arg1	glycomics					66:74	mass spectrometry-based glycomics	42:74	mass spectrometry-based glycomics	42:74	Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.					
32309832	4	39	theme	brain	536:540	arg1	ECM					564:566	ECM	564:566	ECM	564:566	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	4	39	theme	brain	536:540	arg1	matrix					556:561	the brain extracellular matrix	532:561	the brain extracellular matrix (ECM)	532:567	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	4	39	theme	brain	536:540	arg1	interest					520:527	Of particular interest	506:527	Of particular interest	506:527	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	9	40	dep	GAGs	1369:1372	arg1	The					1365:1367	The	1365:1367	The	1365:1367	The GAGs and peptides are then cleaned and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32309832	3	41	theme	important	463:471	arg1	questions					495:503	important biologically relevant questions	463:503	important biologically relevant questions	463:503	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	10	42	theme	lysates	1560:1566	arg1	protocol					1487:1494	This protocol	1482:1494	This protocol	1482:1494	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	10	42	theme	lysates	1560:1566	arg1	way					1527:1529	an efficient and economical way	1499:1529	an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample	1499:1631	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	6	43	theme	complete	1028:1035	arg1	picture					1048:1054	the complete biological picture	1024:1054	the complete biological picture	1024:1054	Thus, there is a growing need for high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture.					
32309832	3	44	gly	Glycosylation	307:319	arg1	processes					382:390	most biological processes	366:390	most biological processes	366:390	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	12	45	with	available	1994:2002	arg1	PXD017513					2040:2048	identifier PXD017513	2029:2048	identifier PXD017513	2029:2048	Overall, we demonstrate a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513).					
32309832	2	46	from	gap	203:205	arg1	analysis					214:221	the analysis	210:221	the analysis of protein post-translational modifications (PTMs)	210:272	However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation.					
32309832	12	47	theme	efficient	1905:1913	arg1	protocol					1915:1922	a high-throughput & efficient protocol	1885:1922	a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513)	1885:2049	Overall, we demonstrate a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513).					
32309832	8	48	theme	filter-aided	1175:1186	arg1	in-solution					1226:1236	a filter-aided sample preparation (FASP) type serial in-solution	1173:1236	a filter-aided sample preparation (FASP) type serial in-solution digestion	1173:1246	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	10	49	theme	efficient	1502:1510	arg1	protocol					1487:1494	This protocol	1482:1494	This protocol	1482:1494	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	10	49	theme	efficient	1502:1510	arg1	way					1527:1529	an efficient and economical way	1499:1529	an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample	1499:1631	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	12	50	theme	proteomic	1965:1973	arg1	analysis					1975:1982	proteomic analysis	1965:1982	proteomic analysis (data are available via ProteomeXchange with identifier PXD017513)	1965:2049	Overall, we demonstrate a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513).					
32309832	8	51	theme	preparation	1195:1205	arg1	in-solution					1226:1236	a filter-aided sample preparation (FASP) type serial in-solution	1173:1236	a filter-aided sample preparation (FASP) type serial in-solution digestion	1173:1246	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	11	52	theme	parallel	1691:1698	arg1	methods					1720:1726	available parallel (multi-pot) release methods	1681:1726	available parallel (multi-pot) release methods	1681:1726	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	5	53	dep	called	743:748	arg1	shown					846:850	shown	846:850	shown to be altered in neuropsychiatric diseases	846:893	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	8	54	theme	FASP	1208:1211	arg1	in-solution					1226:1236	a filter-aided sample preparation (FASP) type serial in-solution	1173:1236	a filter-aided sample preparation (FASP) type serial in-solution digestion	1173:1246	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	2	55	theme	modifications	253:265	arg1	analysis					214:221	the analysis	210:221	the analysis of protein post-translational modifications (PTMs)	210:272	However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation.					
32309832	12	56	theme	identifier	2029:2038	arg1	PXD017513					2040:2048	identifier PXD017513	2029:2048	identifier PXD017513	2029:2048	Overall, we demonstrate a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513).					
32309832	10	57	theme	economical	1516:1525	arg1	protocol					1487:1494	This protocol	1482:1494	This protocol	1482:1494	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	10	57	theme	economical	1516:1525	arg1	way					1527:1529	an efficient and economical way	1499:1529	an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample	1499:1631	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	11	58	theme	release	1712:1718	arg1	methods					1720:1726	available parallel (multi-pot) release methods	1681:1726	available parallel (multi-pot) release methods	1681:1726	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	2	59	theme	protein	226:232	arg1	PTMs					268:271	PTMs	268:271	PTMs	268:271	However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation.					
32309832	2	59	theme	protein	226:232	arg1	modifications					253:265	protein post-translational modifications	226:265	protein post-translational modifications (PTMs)	226:272	However, there remains a gap in the analysis of protein post-translational modifications (PTMs), particularly for glycosylation.					
32309832	8	60	theme	in-solution	1226:1236	arg1	digestion					1238:1246	a filter-aided sample preparation (FASP) type serial in-solution digestion	1173:1246	a filter-aided sample preparation (FASP) type serial in-solution digestion	1173:1246	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	11	61	theme	multi-pot	1701:1709	arg1	methods					1720:1726	available parallel (multi-pot) release methods	1681:1726	available parallel (multi-pot) release methods	1681:1726	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	11	62	dep	efficient	1653:1661	arg1	method					1638:1643	The method	1634:1643	The method	1634:1643	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	11	62	dep	efficient	1653:1661	arg1	efficient					1653:1661	efficient	1653:1661	efficient	1653:1661	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	11	62	dep	efficient	1653:1661	arg1	single-pot					1664:1673	single-pot	1664:1673	single-pot	1664:1673	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	5	63	theme	neuropsychiatric	869:884	arg1	diseases					886:893	neuropsychiatric diseases	869:893	neuropsychiatric diseases	869:893	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	5	64	theme	large	719:723	arg1	structures					732:741	large glycan structures	719:741	large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases	719:893	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	3	65	theme	PTM	346:348	arg1	form					338:341	the most common form	322:341	the most common form of PTM	322:348	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	3	65	theme	PTM	346:348	arg1	Glycosylation					307:319	Glycosylation	307:319	Glycosylation	307:319	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	4	66	theme	Of	506:507	arg1	matrix					556:561	the brain extracellular matrix	532:561	the brain extracellular matrix (ECM)	532:567	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	4	66	theme	Of	506:507	arg1	interest					520:527	Of particular interest	506:527	Of particular interest	506:527	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	8	67	theme	classes	1255:1261	arg1	digestion					1238:1246	a filter-aided sample preparation (FASP) type serial in-solution digestion	1173:1246	a filter-aided sample preparation (FASP) type serial in-solution digestion	1173:1246	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	9	68	theme	chromatography-tandem	1430:1450	arg1	spectrometry					1457:1468	liquid chromatography-tandem mass spectrometry	1423:1468	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1423:1479	The GAGs and peptides are then cleaned and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32309832	9	68	theme	chromatography-tandem	1430:1450	arg1	LC-MS/MS					1471:1478	LC-MS/MS	1471:1478	LC-MS/MS	1471:1478	The GAGs and peptides are then cleaned and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32309832	6	69	theme	high-throughput	930:944	arg1	methods					946:952	high-throughput methods	930:952	high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture	930:1054	Thus, there is a growing need for high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture.					
32309832	5	70	contain	contain	711:717	arg2	structures					732:741	large glycan structures	719:741	large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases	719:893	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	5	70	contain	contain	711:717	arg1	PGs					706:708	PGs	706:708	PGs	706:708	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	5	70	contain	contain	711:717	arg1	proteoglycans					691:703	proteoglycans	691:703	proteoglycans (PGs)	691:709	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	12	71	dep	analysis	1975:1982	arg1	available					1994:2002	available	1994:2002	available	1994:2002	Overall, we demonstrate a high-throughput & efficient protocol for mass spectrometry-based glycomic and proteomic analysis (data are available via ProteomeXchange with identifier PXD017513).					
32309832	7	72	theme	multiple	1132:1139	arg1	classes					1141:1147	multiple classes	1132:1147	multiple classes of biomolecules	1132:1163	The protocol presented here integrates glycomics and proteomics to analyze multiple classes of biomolecules.					
32309832	4	73	theme	Frontier	603:610	arg1	"					611:611	the "final Frontier"	592:611	the "final Frontier"	592:611	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	11	74	theme	proteins	1787:1794	arg1	identification					1765:1778	the identification	1761:1778	the identification of the proteins with higher peptide-coverage	1761:1823	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	0	75	theme	in-solution	7:17	arg1	protocol					29:36	Serial in-solution digestion protocol	0:36	Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.	0:99	Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis.					
32309832	8	76	theme	chondroitin	1291:1301	arg1	sulfate					1303:1309	chondroitin sulfate	1291:1309	chondroitin sulfate (CS)	1291:1314	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	8	76	theme	chondroitin	1291:1301	arg1	CS					1312:1313	CS	1312:1313	CS	1312:1313	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	10	77	theme	processing	1534:1543	arg1	tissue					1545:1550	processing tissue or cell lysates	1534:1566	tissue	1545:1550	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	5	78	theme	crucial	786:792	arg1	components					798:807	crucial ECM components	786:807	crucial ECM components	786:807	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	5	78	theme	crucial	786:792	arg1	nets					833:836	perineuronal nets	820:836	perineuronal nets (PNNs)	820:843	Among these, proteoglycans (PGs) contain large glycan structures called glycosaminoglycans (GAGs) that form crucial ECM components, including perineuronal nets (PNNs), shown to be altered in neuropsychiatric diseases.					
32309832	1	79	theme	mass	116:119	arg1	spectrometry					121:132	mass spectrometry	116:132	mass spectrometry	116:132	Advancement in mass spectrometry has revolutionized the field of proteomics.					
32309832	3	80	theme	biological	371:380	arg1	processes					382:390	most biological processes	366:390	most biological processes	366:390	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	11	81	theme	higher	1801:1806	arg1	peptide-coverage					1808:1823	higher peptide-coverage	1801:1823	higher peptide-coverage	1801:1823	The method is more efficient (single-pot) than available parallel (multi-pot) release methods, and removal of GAGs facilitates the identification of the proteins with higher peptide-coverage than using conventional-proteomics.					
32309832	4	82	theme	extracellular	542:554	arg1	ECM					564:566	ECM	564:566	ECM	564:566	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	4	82	theme	extracellular	542:554	arg1	matrix					556:561	the brain extracellular matrix	532:561	the brain extracellular matrix (ECM)	532:567	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	4	82	theme	extracellular	542:554	arg1	interest					520:527	Of particular interest	506:527	Of particular interest	506:527	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	10	83	theme	GAG	1587:1589	arg1	classes					1591:1597	various GAG classes	1579:1597	various GAG classes	1579:1597	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
32309832	1	84	from	Advancement	101:111	arg1	spectrometry					121:132	mass spectrometry	116:132	mass spectrometry	116:132	Advancement in mass spectrometry has revolutionized the field of proteomics.					
32309832	8	85	theme	type	1214:1217	arg1	in-solution					1226:1236	a filter-aided sample preparation (FASP) type serial in-solution	1173:1236	a filter-aided sample preparation (FASP) type serial in-solution digestion	1173:1246	We use a filter-aided sample preparation (FASP) type serial in-solution digestion of GAG classes, including hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS), followed by peptides.					
32309832	6	86	theme	biological	1037:1046	arg1	picture					1048:1054	the complete biological picture	1024:1054	the complete biological picture	1024:1054	Thus, there is a growing need for high-throughput methods that combine GAG (glycomics) and PGs (proteomics) analysis to unravel the complete biological picture.					
32309832	3	87	theme	proteins	430:437	arg1	analysis					399:406	analysis	399:406	analysis of glycans along with proteins	399:437	Glycosylation, the most common form of PTM, is involved in most biological processes; thus, analysis of glycans along with proteins is crucial to answering important biologically relevant questions.					
32309832	4	88	theme	final	597:601	arg1	"					611:611	the "final Frontier"	592:611	the "final Frontier"	592:611	Of particular interest is the brain extracellular matrix (ECM), which has been called the "final Frontier" in neuroscience, which consists of highly glycosylated proteins.					
32309832	10	89	theme	same	1621:1624	arg1	sample					1626:1631	the same sample	1617:1631	the same sample	1617:1631	This protocol is an efficient and economical way of processing tissue or cell lysates to isolate various GAG classes and peptides from the same sample.					
33793242	4	0	theme	resonance	498:506	arg1	studies					518:524	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies	450:524	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies	450:524	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	2	1	theme	thio	280:283	arg1	linkage					285:291	a thio linkage	278:291	a thio linkage	278:291	Herein, we report the synthesis of oligosaccharides containing a thio linkage at the nonreducing end.					
33793242	4	2	theme	STD	509:511	arg1	resonance					498:506	Saturation transfer difference nuclear magnetic resonance	450:506	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies	450:524	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	4	2	theme	STD	509:511	arg1	NMR					513:515	STD NMR	509:515	STD NMR	509:515	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	0	3	theme	Man4	41:44	arg1	Glycans					46:52	Mannosidase-Stable Man3 and Man4 Glycans	13:52	Mannosidase-Stable Man3 and Man4 Glycans	13:52	Synthesis of Mannosidase-Stable Man3 and Man4 Glycans Containing S-linked Manα1→2Man Termini.					
33793242	4	4	theme	manihotis	677:685	arg1	mannosidase					687:697	x. manihotis mannosidase	674:697	x. manihotis mannosidase	674:697	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	3	5	theme	thio-linked	319:329	arg1	donor					341:345	A thio-linked dimannose donor	317:345	A thio-linked dimannose donor	317:345	A thio-linked dimannose donor participates in highly stereoselective glycosylations to afford trimannose and tetramannose fragments.					
33793242	1	6	theme	Oligomannose	94:105	arg1	glycans					107:113	Oligomannose glycans	94:113	Oligomannose glycans	94:113	Oligomannose glycans are of interest as HIV vaccine components, but they are subject to mannosidase degradation in vivo.					
33793242	2	7	contain	containing	267:276	arg2	linkage					285:291	a thio linkage	278:291	a thio linkage	278:291	Herein, we report the synthesis of oligosaccharides containing a thio linkage at the nonreducing end.					
33793242	2	7	contain	containing	267:276	arg1	oligosaccharides					250:265	oligosaccharides	250:265	oligosaccharides containing a thio linkage at the nonreducing end	250:314	Herein, we report the synthesis of oligosaccharides containing a thio linkage at the nonreducing end.					
33793242	2	8	theme	nonreducing	300:310	arg1	end					312:314	the nonreducing end	296:314	the nonreducing end	296:314	Herein, we report the synthesis of oligosaccharides containing a thio linkage at the nonreducing end.					
33793242	1	9	theme	mannosidase	182:192	arg1	degradation					194:204	mannosidase degradation	182:204	mannosidase degradation	182:204	Oligomannose glycans are of interest as HIV vaccine components, but they are subject to mannosidase degradation in vivo.					
33793242	3	10	theme	stereoselective	370:384	arg1	glycosylations					386:399	highly stereoselective glycosylations	363:399	highly stereoselective glycosylations	363:399	A thio-linked dimannose donor participates in highly stereoselective glycosylations to afford trimannose and tetramannose fragments.					
33793242	3	11	link	thio-linked	319:329	arg1	donor					341:345	A thio-linked dimannose donor	317:345	A thio-linked dimannose donor	317:345	A thio-linked dimannose donor participates in highly stereoselective glycosylations to afford trimannose and tetramannose fragments.					
33793242	0	12	theme	Mannosidase-Stable	13:30	arg1	Glycans					46:52	Mannosidase-Stable Man3 and Man4 Glycans	13:52	Mannosidase-Stable Man3 and Man4 Glycans	13:52	Synthesis of Mannosidase-Stable Man3 and Man4 Glycans Containing S-linked Manα1→2Man Termini.					
33793242	4	13	theme	magnetic	489:496	arg1	resonance					498:506	Saturation transfer difference nuclear magnetic resonance	450:506	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies	450:524	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	4	13	theme	magnetic	489:496	arg1	NMR					513:515	STD NMR	509:515	STD NMR	509:515	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	4	14	theme	complete	647:654	arg1	stability					656:664	complete stability	647:664	complete stability against x. manihotis mannosidase	647:697	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	3	15	theme	tetramannose	426:437	arg1	fragments					439:447	tetramannose fragments	426:447	tetramannose fragments	426:447	A thio-linked dimannose donor participates in highly stereoselective glycosylations to afford trimannose and tetramannose fragments.					
33793242	4	16	theme	nuclear	481:487	arg1	resonance					498:506	Saturation transfer difference nuclear magnetic resonance	450:506	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies	450:524	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	4	16	theme	nuclear	481:487	arg1	NMR					513:515	STD NMR	509:515	STD NMR	509:515	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	4	17	theme	x.	674:675	arg1	mannosidase					687:697	x. manihotis mannosidase	674:697	x. manihotis mannosidase	674:697	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	0	18	theme	Man3	32:35	arg1	Glycans					46:52	Mannosidase-Stable Man3 and Man4 Glycans	13:52	Mannosidase-Stable Man3 and Man4 Glycans	13:52	Synthesis of Mannosidase-Stable Man3 and Man4 Glycans Containing S-linked Manα1→2Man Termini.					
33793242	0	19	theme	S-linked	65:72	arg1	Termini					85:91	S-linked Manα1→2Man Termini	65:91	S-linked Manα1→2Man Termini	65:91	Synthesis of Mannosidase-Stable Man3 and Man4 Glycans Containing S-linked Manα1→2Man Termini.					
33793242	0	20	theme	Glycans	46:52	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Mannosidase-Stable Man3 and Man4 Glycans	0:52	Synthesis of Mannosidase-Stable Man3 and Man4 Glycans Containing S-linked Manα1→2Man Termini.					
33793242	4	21	theme	HIV	568:570	arg1	antibody					572:579	HIV antibody 2G12	568:584	HIV antibody 2G12	568:584	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	4	22	theme	Saturation	450:459	arg1	resonance					498:506	Saturation transfer difference nuclear magnetic resonance	450:506	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies	450:524	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	4	22	theme	Saturation	450:459	arg1	NMR					513:515	STD NMR	509:515	STD NMR	509:515	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	2	23	theme	oligosaccharides	250:265	arg1	synthesis					237:245	the synthesis	233:245	the synthesis of oligosaccharides containing a thio linkage at the nonreducing end	233:314	Herein, we report the synthesis of oligosaccharides containing a thio linkage at the nonreducing end.					
33793242	0	24	link	S-linked	65:72	arg1	Termini					85:91	S-linked Manα1→2Man Termini	65:91	S-linked Manα1→2Man Termini	65:91	Synthesis of Mannosidase-Stable Man3 and Man4 Glycans Containing S-linked Manα1→2Man Termini.					
33793242	1	25	theme	HIV	134:136	arg1	components					146:155	HIV vaccine components	134:155	HIV vaccine components	134:155	Oligomannose glycans are of interest as HIV vaccine components, but they are subject to mannosidase degradation in vivo.					
33793242	4	26	theme	terminal	620:627	arg1	S-linkage					629:637	the reducing terminal S-linkage	607:637	the reducing terminal S-linkage	607:637	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	4	27	theme	difference	470:479	arg1	resonance					498:506	Saturation transfer difference nuclear magnetic resonance	450:506	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies	450:524	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	4	27	theme	difference	470:479	arg1	NMR					513:515	STD NMR	509:515	STD NMR	509:515	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	1	28	theme	vaccine	138:144	arg1	components					146:155	HIV vaccine components	134:155	HIV vaccine components	134:155	Oligomannose glycans are of interest as HIV vaccine components, but they are subject to mannosidase degradation in vivo.					
33793242	4	29	theme	reducing	611:618	arg1	S-linkage					629:637	the reducing terminal S-linkage	607:637	the reducing terminal S-linkage	607:637	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	0	30	theme	Manα1→2Man	74:83	arg1	Termini					85:91	S-linked Manα1→2Man Termini	65:91	S-linked Manα1→2Man Termini	65:91	Synthesis of Mannosidase-Stable Man3 and Man4 Glycans Containing S-linked Manα1→2Man Termini.					
33793242	4	31	theme	transfer	461:468	arg1	resonance					498:506	Saturation transfer difference nuclear magnetic resonance	450:506	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies	450:524	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	4	31	theme	transfer	461:468	arg1	NMR					513:515	STD NMR	509:515	STD NMR	509:515	Saturation transfer difference nuclear magnetic resonance (STD NMR) studies show that these glycans are recognized by HIV antibody 2G12, and we confirm that the reducing terminal S-linkage confers complete stability against x. manihotis mannosidase.					
33793242	3	32	theme	dimannose	331:339	arg1	donor					341:345	A thio-linked dimannose donor	317:345	A thio-linked dimannose donor	317:345	A thio-linked dimannose donor participates in highly stereoselective glycosylations to afford trimannose and tetramannose fragments.					
32978395	1	0	theme	complex	173:179	arg1	molecules					181:189	complex molecules	173:189	complex molecules	173:189	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	0	1	theme	stereoselective	74:88	arg1	reactions					90:98	regio- and stereoselective reactions	63:98	regio- and stereoselective reactions on carbohydrates	63:115	Transfer learning enables the molecular transformer to predict regio- and stereoselective reactions on carbohydrates.					
32978395	5	2	link	lipid-linked	1052:1063	arg1	oligosaccharide					1065:1079	a lipid-linked oligosaccharide	1050:1079	a lipid-linked oligosaccharide involving regioselective protections and stereoselective glycosylations	1050:1151	We validate these predictions experimentally with the synthesis of a lipid-linked oligosaccharide involving regioselective protections and stereoselective glycosylations.					
32978395	1	3	theme	molecules	181:189	arg1	synthesis					160:168	the synthesis	156:168	the synthesis of complex molecules and materials used in all fields of science and technology	156:248	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	1	4	theme	vast	267:270	arg1	body					272:275	a vast body	265:275	a vast body of accumulated knowledge optimally suited for deep learning	265:335	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	3	5	from	reactions	668:676	arg1	carbohydrates					681:693	carbohydrates	681:693	carbohydrates where regio- and stereoselectivity are notoriously difficult to predict	681:765	Here, we challenge the Molecular Transformer model to predict reactions on carbohydrates where regio- and stereoselectivity are notoriously difficult to predict.					
32978395	4	6	theme	patent	814:819	arg1	model					830:834	the general patent reaction model	802:834	the general patent reaction model	802:834	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	4	7	theme	carbohydrate	934:945	arg1	reactions					947:955	carbohydrate reactions	934:955	carbohydrate reactions with remarkable accuracy	934:980	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	2	8	theme	distinct	375:382	arg1	groups					395:400	distinct functional groups	375:400	distinct functional groups	375:400	While most organic reactions involve distinct functional groups and can readily be learned by deep learning models and chemists alike, regio- and stereoselective transformations are more challenging because their outcome also depends on functional group surroundings.					
32978395	4	9	theme	specialized	890:900	arg1	predictions					918:928	a specialized model returning predictions	888:928	a specialized model returning predictions for carbohydrate reactions with remarkable accuracy	888:980	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	4	10	theme	model	830:834	arg1	learning					790:797	transfer learning	781:797	transfer learning of the general patent reaction model with a small set of carbohydrate reactions	781:877	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	6	11	theme	transfer	1158:1165	arg1	approach					1176:1183	The transfer learning approach	1154:1183	The transfer learning approach	1154:1183	The transfer learning approach should be applicable to any reaction class of interest.					
32978395	6	11	theme	transfer	1158:1165	arg1	applicable					1195:1204	applicable	1195:1204	applicable	1195:1204	The transfer learning approach should be applicable to any reaction class of interest.					
32978395	4	12	theme	reaction	821:828	arg1	model					830:834	the general patent reaction model	802:834	the general patent reaction model	802:834	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	6	13	theme	reaction	1213:1220	arg1	class					1222:1226	any reaction class	1209:1226	any reaction class of interest	1209:1238	The transfer learning approach should be applicable to any reaction class of interest.					
32978395	1	14	theme	materials	195:203	arg1	synthesis					160:168	the synthesis	156:168	the synthesis of complex molecules and materials used in all fields of science and technology	156:248	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	4	15	theme	returning	908:916	arg1	predictions					918:928	a specialized model returning predictions	888:928	a specialized model returning predictions for carbohydrate reactions with remarkable accuracy	888:980	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	0	16	theme	Transfer	0:7	arg1	learning					9:16	Transfer learning	0:16	Transfer learning	0:16	Transfer learning enables the molecular transformer to predict regio- and stereoselective reactions on carbohydrates.					
32978395	1	17	theme	accumulated	280:290	arg1	knowledge					292:300	accumulated knowledge	280:300	accumulated knowledge optimally suited for deep learning	280:335	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	4	18	theme	model	902:906	arg1	predictions					918:928	a specialized model returning predictions	888:928	a specialized model returning predictions for carbohydrate reactions with remarkable accuracy	888:980	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	4	19	theme	transfer	781:788	arg1	learning					790:797	transfer learning	781:797	transfer learning of the general patent reaction model with a small set of carbohydrate reactions	781:877	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	1	20	theme	knowledge	292:300	arg1	body					272:275	a vast body	265:275	a vast body of accumulated knowledge optimally suited for deep learning	265:335	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	2	21	theme	group	586:590	arg1	surroundings					592:603	functional group surroundings	575:603	functional group surroundings	575:603	While most organic reactions involve distinct functional groups and can readily be learned by deep learning models and chemists alike, regio- and stereoselective transformations are more challenging because their outcome also depends on functional group surroundings.					
32978395	5	22	theme	stereoselective	1122:1136	arg1	glycosylations					1138:1151	stereoselective glycosylations	1122:1151	stereoselective glycosylations	1122:1151	We validate these predictions experimentally with the synthesis of a lipid-linked oligosaccharide involving regioselective protections and stereoselective glycosylations.					
32978395	2	23	theme	learning	437:444	arg1	models					446:451	deep learning models	432:451	deep learning models	432:451	While most organic reactions involve distinct functional groups and can readily be learned by deep learning models and chemists alike, regio- and stereoselective transformations are more challenging because their outcome also depends on functional group surroundings.					
32978395	2	24	theme	functional	575:584	arg1	surroundings					592:603	functional group surroundings	575:603	functional group surroundings	575:603	While most organic reactions involve distinct functional groups and can readily be learned by deep learning models and chemists alike, regio- and stereoselective transformations are more challenging because their outcome also depends on functional group surroundings.					
32978395	0	25	from	reactions	90:98	arg1	carbohydrates					103:115	carbohydrates	103:115	carbohydrates	103:115	Transfer learning enables the molecular transformer to predict regio- and stereoselective reactions on carbohydrates.					
32978395	1	26	theme	Organic	118:124	arg1	methodology					136:146	Organic synthesis methodology	118:146	Organic synthesis methodology	118:146	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	2	27	theme	deep	432:435	arg1	models					446:451	deep learning models	432:451	deep learning models	432:451	While most organic reactions involve distinct functional groups and can readily be learned by deep learning models and chemists alike, regio- and stereoselective transformations are more challenging because their outcome also depends on functional group surroundings.					
32978395	2	28	theme	stereoselective	484:498	arg1	transformations					500:514	regio- and stereoselective transformations	473:514	regio- and stereoselective transformations	473:514	While most organic reactions involve distinct functional groups and can readily be learned by deep learning models and chemists alike, regio- and stereoselective transformations are more challenging because their outcome also depends on functional group surroundings.					
32978395	4	29	theme	carbohydrate	856:867	arg1	reactions					869:877	carbohydrate reactions	856:877	carbohydrate reactions	856:877	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	6	30	theme	learning	1167:1174	arg1	approach					1176:1183	The transfer learning approach	1154:1183	The transfer learning approach	1154:1183	The transfer learning approach should be applicable to any reaction class of interest.					
32978395	6	30	theme	learning	1167:1174	arg1	applicable					1195:1204	applicable	1195:1204	applicable	1195:1204	The transfer learning approach should be applicable to any reaction class of interest.					
32978395	0	31	theme	molecular	30:38	arg1	transformer					40:50	the molecular transformer	26:50	the molecular transformer to predict regio- and stereoselective reactions on carbohydrates	26:115	Transfer learning enables the molecular transformer to predict regio- and stereoselective reactions on carbohydrates.					
32978395	4	32	theme	general	806:812	arg1	model					830:834	the general patent reaction model	802:834	the general patent reaction model	802:834	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	3	33	theme	Molecular	629:637	arg1	model					651:655	the Molecular Transformer model	625:655	the Molecular Transformer model to predict reactions on carbohydrates where regio- and stereoselectivity are notoriously difficult to predict	625:765	Here, we challenge the Molecular Transformer model to predict reactions on carbohydrates where regio- and stereoselectivity are notoriously difficult to predict.					
32978395	1	34	theme	science	227:233	arg1	fields					217:222	all fields	213:222	all fields of science and technology	213:248	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	2	35	theme	regio-	473:478	arg1	transformations					500:514	regio- and stereoselective transformations	473:514	regio- and stereoselective transformations	473:514	While most organic reactions involve distinct functional groups and can readily be learned by deep learning models and chemists alike, regio- and stereoselective transformations are more challenging because their outcome also depends on functional group surroundings.					
32978395	1	36	theme	deep	323:326	arg1	learning					328:335	deep learning	323:335	deep learning	323:335	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	4	37	theme	remarkable	962:971	arg1	accuracy					973:980	remarkable accuracy	962:980	remarkable accuracy	962:980	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	1	38	theme	synthesis	126:134	arg1	methodology					136:146	Organic synthesis methodology	118:146	Organic synthesis methodology	118:146	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	4	39	with	learning	790:797	arg1	set					849:851	a small set	841:851	a small set of carbohydrate reactions	841:877	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	4	39	with	learning	790:797	arg1	reactions					869:877	carbohydrate reactions	856:877	carbohydrate reactions	856:877	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	4	40	theme	reactions	869:877	arg1	set					849:851	a small set	841:851	a small set of carbohydrate reactions	841:877	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	4	40	theme	reactions	869:877	arg1	reactions					869:877	carbohydrate reactions	856:877	carbohydrate reactions	856:877	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	2	41	theme	organic	349:355	arg1	reactions					357:365	most organic reactions	344:365	most organic reactions	344:365	While most organic reactions involve distinct functional groups and can readily be learned by deep learning models and chemists alike, regio- and stereoselective transformations are more challenging because their outcome also depends on functional group surroundings.					
32978395	4	42	with	reactions	947:955	arg1	accuracy					973:980	remarkable accuracy	962:980	remarkable accuracy	962:980	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	2	43	theme	most	344:347	arg1	reactions					357:365	most organic reactions	344:365	most organic reactions	344:365	While most organic reactions involve distinct functional groups and can readily be learned by deep learning models and chemists alike, regio- and stereoselective transformations are more challenging because their outcome also depends on functional group surroundings.					
32978395	5	44	theme	oligosaccharide	1065:1079	arg1	synthesis					1037:1045	the synthesis	1033:1045	the synthesis of a lipid-linked oligosaccharide involving regioselective protections and stereoselective glycosylations	1033:1151	We validate these predictions experimentally with the synthesis of a lipid-linked oligosaccharide involving regioselective protections and stereoselective glycosylations.					
32978395	5	45	theme	regioselective	1091:1104	arg1	protections					1106:1116	regioselective protections	1091:1116	regioselective protections	1091:1116	We validate these predictions experimentally with the synthesis of a lipid-linked oligosaccharide involving regioselective protections and stereoselective glycosylations.					
32978395	1	46	theme	technology	239:248	arg1	fields					217:222	all fields	213:222	all fields of science and technology	213:248	Organic synthesis methodology enables the synthesis of complex molecules and materials used in all fields of science and technology and represents a vast body of accumulated knowledge optimally suited for deep learning.					
32978395	5	47	theme	lipid-linked	1052:1063	arg1	oligosaccharide					1065:1079	a lipid-linked oligosaccharide	1050:1079	a lipid-linked oligosaccharide involving regioselective protections and stereoselective glycosylations	1050:1151	We validate these predictions experimentally with the synthesis of a lipid-linked oligosaccharide involving regioselective protections and stereoselective glycosylations.					
32978395	3	48	theme	Transformer	639:649	arg1	model					651:655	the Molecular Transformer model	625:655	the Molecular Transformer model to predict reactions on carbohydrates where regio- and stereoselectivity are notoriously difficult to predict	625:765	Here, we challenge the Molecular Transformer model to predict reactions on carbohydrates where regio- and stereoselectivity are notoriously difficult to predict.					
32978395	6	49	theme	interest	1231:1238	arg1	class					1222:1226	any reaction class	1209:1226	any reaction class of interest	1209:1238	The transfer learning approach should be applicable to any reaction class of interest.					
32978395	0	50	theme	regio-	63:68	arg1	reactions					90:98	regio- and stereoselective reactions	63:98	regio- and stereoselective reactions on carbohydrates	63:115	Transfer learning enables the molecular transformer to predict regio- and stereoselective reactions on carbohydrates.					
32978395	4	51	theme	small	843:847	arg1	set					849:851	a small set	841:851	a small set of carbohydrate reactions	841:877	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	4	51	theme	small	843:847	arg1	reactions					869:877	carbohydrate reactions	856:877	carbohydrate reactions	856:877	We show that transfer learning of the general patent reaction model with a small set of carbohydrate reactions produces a specialized model returning predictions for carbohydrate reactions with remarkable accuracy.					
32978395	2	52	theme	functional	384:393	arg1	groups					395:400	distinct functional groups	375:400	distinct functional groups	375:400	While most organic reactions involve distinct functional groups and can readily be learned by deep learning models and chemists alike, regio- and stereoselective transformations are more challenging because their outcome also depends on functional group surroundings.					
32955262	4	0	from	2.6	685:687	arg1	optimum					674:680	a pH optimum	669:680	a pH optimum at 2.6	669:687	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	3	1	theme	regions	578:584	arg1	analysis					543:550	HDX-MS analysis	536:550	HDX-MS analysis of N-glycosylated protein regions	536:584	We have recently demonstrated the utility of the glycosidase PNGase A to enable HDX-MS analysis of N-glycosylated protein regions.					
32955262	9	2	theme	unique	1447:1452	arg1	features					1454:1461	the unique features	1443:1461	the unique features of PNGase H+	1443:1474	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	6	3	theme	HDX-MS	903:908	arg1	workflow					910:917	an HDX-MS workflow	900:917	an HDX-MS workflow	900:917	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	7	4	theme	deglycosylation	1101:1115	arg1	performance					1117:1127	similar deglycosylation performance	1093:1127	similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ)	1093:1212	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	3	5	theme	N-glycosylated	555:568	arg1	regions					578:584	N-glycosylated protein regions	555:584	N-glycosylated protein regions	555:584	We have recently demonstrated the utility of the glycosidase PNGase A to enable HDX-MS analysis of N-glycosylated protein regions.					
32955262	8	6	theme	greater	1267:1273	arg1	tolerance					1275:1283	greater tolerance	1267:1283	greater tolerance to the disulfide-bond reducing agent TCEP	1267:1325	However, PNGase H+ provides broader specificity and greater tolerance to the disulfide-bond reducing agent TCEP, while PNGase A offers advantages in terms of commercial availability and purity.					
32955262	9	7	theme	H+	1473:1474	arg1	features					1454:1461	the unique features	1443:1461	the unique features of PNGase H+	1443:1474	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	4	8	theme	H+	655:656	arg1	use					618:620	the use	614:620	the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins	614:788	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	4	9	gly	deglycosylate	705:717	arg1	peptides					741:748	N-linked glycosylated peptides	719:748	N-linked glycosylated peptides	719:748	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	8	10	theme	PNGase	1224:1229	arg1	H+					1231:1232	PNGase H+	1224:1232	PNGase H+	1224:1232	However, PNGase H+ provides broader specificity and greater tolerance to the disulfide-bond reducing agent TCEP, while PNGase A offers advantages in terms of commercial availability and purity.					
32955262	6	11	theme	data	960:963	arg1	extraction					942:951	the extraction	938:951	the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin	938:1032	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	8	12	theme	disulfide-bond	1292:1305	arg1	TCEP					1322:1325	the disulfide-bond reducing agent TCEP	1288:1325	the disulfide-bond reducing agent TCEP	1288:1325	However, PNGase H+ provides broader specificity and greater tolerance to the disulfide-bond reducing agent TCEP, while PNGase A offers advantages in terms of commercial availability and purity.					
32955262	7	13	theme	PNGase	1068:1073	arg1	H+					1075:1076	PNGase H+	1068:1076	PNGase H+	1068:1076	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	5	14	theme	high	831:834	arg1	activity					852:859	high deglycosylation activity	831:859	high deglycosylation activity	831:859	Our results show that PNGase H+ retains high deglycosylation activity at HDX quench conditions.					
32955262	5	15	theme	quench	868:873	arg1	conditions					875:884	HDX quench conditions	864:884	HDX quench conditions	864:884	Our results show that PNGase H+ retains high deglycosylation activity at HDX quench conditions.					
32955262	8	16	theme	agent	1316:1320	arg1	TCEP					1322:1325	the disulfide-bond reducing agent TCEP	1288:1325	the disulfide-bond reducing agent TCEP	1288:1325	However, PNGase H+ provides broader specificity and greater tolerance to the disulfide-bond reducing agent TCEP, while PNGase A offers advantages in terms of commercial availability and purity.					
32955262	9	17	theme	glycoproteins	1517:1529	arg1	analysis					1505:1512	conformational analysis	1490:1512	conformational analysis of glycoproteins	1490:1529	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	4	18	theme	glycosylated	728:739	arg1	peptides					741:748	N-linked glycosylated peptides	719:748	N-linked glycosylated peptides	719:748	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	8	19	theme	PNGase	1334:1339	arg1	A					1341:1341	PNGase A	1334:1341	PNGase A	1334:1341	However, PNGase H+ provides broader specificity and greater tolerance to the disulfide-bond reducing agent TCEP, while PNGase A offers advantages in terms of commercial availability and purity.					
32955262	4	20	gly	glycoproteins	776:788	arg1	glycoproteins					776:788	glycoproteins	776:788	glycoproteins	776:788	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	5	21	theme	deglycosylation	836:850	arg1	activity					852:859	high deglycosylation activity	831:859	high deglycosylation activity	831:859	Our results show that PNGase H+ retains high deglycosylation activity at HDX quench conditions.					
32955262	6	22	theme	serpin	1007:1012	arg1	α1-antichymotrypsin					1014:1032	the serpin α1-antichymotrypsin	1003:1032	the serpin α1-antichymotrypsin	1003:1032	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	3	23	gly	N-glycosylated	555:568	arg1	regions					578:584	N-glycosylated protein regions	555:584	N-glycosylated protein regions	555:584	We have recently demonstrated the utility of the glycosidase PNGase A to enable HDX-MS analysis of N-glycosylated protein regions.					
32955262	0	24	theme	N-Linked	72:79	arg1	Glycoproteins					81:93	N-Linked Glycoproteins	72:93	N-Linked Glycoproteins	72:93	Deglycosylation by the Acidic Glycosidase PNGase H+ Enables Analysis of N-Linked Glycoproteins by Hydrogen/Deuterium Exchange Mass Spectrometry.					
32955262	4	25	theme	glycoproteins	776:788	arg1	analysis					764:771	HDX-MS analysis	757:771	HDX-MS analysis of glycoproteins	757:788	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	6	26	theme	glycosylated	979:990	arg1	regions					992:998	all five glycosylated regions	970:998	all five glycosylated regions of the serpin α1-antichymotrypsin	970:1032	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	6	27	from	regions	992:998	arg1	extraction					942:951	the extraction	938:951	the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin	938:1032	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	6	27	from	regions	992:998	arg1	data					960:963	HDX data	956:963	HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin	956:1032	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	2	28	theme	segments	422:429	arg1	uptake					400:405	the deuterium uptake	386:405	the deuterium uptake of the protein segments that contain the glycan	386:453	However, glycoproteins represent a challenge to the traditional HDX-MS workflow for determining the deuterium uptake of the protein segments that contain the glycan.					
32955262	0	29	theme	Exchange	117:124	arg1	Spectrometry					131:142	Hydrogen/Deuterium Exchange Mass Spectrometry	98:142	Hydrogen/Deuterium Exchange Mass Spectrometry	98:142	Deglycosylation by the Acidic Glycosidase PNGase H+ Enables Analysis of N-Linked Glycoproteins by Hydrogen/Deuterium Exchange Mass Spectrometry.					
32955262	8	30	theme	availability	1384:1395	arg1	terms					1364:1368	terms	1364:1368	terms of commercial availability and purity	1364:1406	However, PNGase H+ provides broader specificity and greater tolerance to the disulfide-bond reducing agent TCEP, while PNGase A offers advantages in terms of commercial availability and purity.					
32955262	8	31	theme	purity	1401:1406	arg1	terms					1364:1368	terms	1364:1368	terms of commercial availability and purity	1364:1406	However, PNGase H+ provides broader specificity and greater tolerance to the disulfide-bond reducing agent TCEP, while PNGase A offers advantages in terms of commercial availability and purity.					
32955262	7	32	theme	antibody	1188:1195	arg1	TZ					1210:1211	TZ	1210:1211	TZ	1210:1211	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	7	32	theme	antibody	1188:1195	arg1	trastuzumab					1197:1207	the IgG1 antibody trastuzumab	1179:1207	the IgG1 antibody trastuzumab (TZ)	1179:1212	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	0	33	theme	Glycosidase	30:40	arg1	H+					49:50	the Acidic Glycosidase PNGase H+	19:50	the Acidic Glycosidase PNGase H+	19:50	Deglycosylation by the Acidic Glycosidase PNGase H+ Enables Analysis of N-Linked Glycoproteins by Hydrogen/Deuterium Exchange Mass Spectrometry.					
32955262	6	34	from	extraction	942:951	arg1	regions					992:998	all five glycosylated regions	970:998	all five glycosylated regions of the serpin α1-antichymotrypsin	970:1032	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	3	35	theme	PNGase	517:522	arg1	A					524:524	the glycosidase PNGase A	501:524	the glycosidase PNGase A	501:524	We have recently demonstrated the utility of the glycosidase PNGase A to enable HDX-MS analysis of N-glycosylated protein regions.					
32955262	5	36	theme	PNGase	813:818	arg1	H+					820:821	PNGase H+	813:821	PNGase H+	813:821	Our results show that PNGase H+ retains high deglycosylation activity at HDX quench conditions.					
32955262	2	37	theme	HDX-MS	354:359	arg1	workflow					361:368	the traditional HDX-MS workflow	338:368	the traditional HDX-MS workflow for determining the deuterium uptake of the protein segments that contain the glycan	338:453	However, glycoproteins represent a challenge to the traditional HDX-MS workflow for determining the deuterium uptake of the protein segments that contain the glycan.					
32955262	3	38	theme	HDX-MS	536:541	arg1	analysis					543:550	HDX-MS analysis	536:550	HDX-MS analysis of N-glycosylated protein regions	536:584	We have recently demonstrated the utility of the glycosidase PNGase A to enable HDX-MS analysis of N-glycosylated protein regions.					
32955262	4	39	theme	acidic	629:634	arg1	H+					655:656	the acidic glycosidase PNGase H+	625:656	the acidic glycosidase PNGase H+	625:656	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	7	40	theme	HDX-MS	1136:1141	arg1	analysis					1143:1150	HDX-MS analysis	1136:1150	HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ)	1136:1212	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	6	41	theme	PNGase	920:925	arg1	H+					927:928	PNGase H+	920:928	PNGase H+	920:928	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	2	42	gly	glycoproteins	299:311	arg1	glycoproteins					299:311	glycoproteins	299:311	glycoproteins	299:311	However, glycoproteins represent a challenge to the traditional HDX-MS workflow for determining the deuterium uptake of the protein segments that contain the glycan.					
32955262	7	43	theme	performance	1117:1127	arg1	capable					1082:1088	capable	1082:1088	capable	1082:1088	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	3	44	theme	protein	570:576	arg1	regions					578:584	N-glycosylated protein regions	555:584	N-glycosylated protein regions	555:584	We have recently demonstrated the utility of the glycosidase PNGase A to enable HDX-MS analysis of N-glycosylated protein regions.					
32955262	1	45	theme	proteins	280:287	arg1	dynamics					268:275	the structural dynamics	253:275	the structural dynamics of proteins	253:287	Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) has become an important method to study the structural dynamics of proteins.					
32955262	4	46	link	N-linked	719:726	arg1	peptides					741:748	N-linked glycosylated peptides	719:748	N-linked glycosylated peptides	719:748	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	7	47	theme	similar	1093:1099	arg1	performance					1117:1127	similar deglycosylation performance	1093:1127	similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ)	1093:1212	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	7	48	theme	α1-antichymotrypsin	1155:1173	arg1	analysis					1143:1150	HDX-MS analysis	1136:1150	HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ)	1136:1212	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	8	49	theme	broader	1243:1249	arg1	specificity					1251:1261	broader specificity	1243:1261	broader specificity	1243:1261	However, PNGase H+ provides broader specificity and greater tolerance to the disulfide-bond reducing agent TCEP, while PNGase A offers advantages in terms of commercial availability and purity.					
32955262	9	50	theme	PNGase	1466:1471	arg1	H+					1473:1474	PNGase H+	1466:1474	PNGase H+	1466:1474	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	4	51	theme	pH	671:672	arg1	optimum					674:680	a pH optimum	669:680	a pH optimum at 2.6	669:687	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	4	52	theme	PNGase	648:653	arg1	H+					655:656	the acidic glycosidase PNGase H+	625:656	the acidic glycosidase PNGase H+	625:656	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	5	53	theme	HDX	864:866	arg1	conditions					875:884	HDX quench conditions	864:884	HDX quench conditions	864:884	Our results show that PNGase H+ retains high deglycosylation activity at HDX quench conditions.					
32955262	6	54	theme	HDX	956:958	arg1	data					960:963	HDX data	956:963	HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin	956:1032	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	7	55	theme	PNGase	1055:1060	arg1	A					1062:1062	PNGase A	1055:1062	PNGase A	1055:1062	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	8	56	theme	reducing	1307:1314	arg1	TCEP					1322:1325	the disulfide-bond reducing agent TCEP	1288:1325	the disulfide-bond reducing agent TCEP	1288:1325	However, PNGase H+ provides broader specificity and greater tolerance to the disulfide-bond reducing agent TCEP, while PNGase A offers advantages in terms of commercial availability and purity.					
32955262	7	57	theme	IgG1	1183:1186	arg1	antibody					1188:1195	the IgG1 antibody	1179:1195	the IgG1 antibody trastuzumab (TZ)	1179:1212	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	2	58	contain	contain	436:442	arg2	glycan					448:453	the glycan	444:453	the glycan	444:453	However, glycoproteins represent a challenge to the traditional HDX-MS workflow for determining the deuterium uptake of the protein segments that contain the glycan.					
32955262	2	58	contain	contain	436:442	arg1	segments					422:429	the protein segments	410:429	the protein segments that contain the glycan	410:453	However, glycoproteins represent a challenge to the traditional HDX-MS workflow for determining the deuterium uptake of the protein segments that contain the glycan.					
32955262	1	59	theme	Hydrogen/deuterium	145:162	arg1	exchange					164:171	Hydrogen/deuterium exchange	145:171	Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS)	145:211	Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) has become an important method to study the structural dynamics of proteins.					
32955262	4	60	theme	N-linked	719:726	arg1	peptides					741:748	N-linked glycosylated peptides	719:748	N-linked glycosylated peptides	719:748	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	6	61	theme	α1-antichymotrypsin	1014:1032	arg1	regions					992:998	all five glycosylated regions	970:998	all five glycosylated regions of the serpin α1-antichymotrypsin	970:1032	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	9	62	gly	glycoproteins	1517:1529	arg1	glycoproteins					1517:1529	glycoproteins	1517:1529	glycoproteins	1517:1529	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	0	63	theme	Glycoproteins	81:93	arg1	Analysis					60:67	Analysis	60:67	Analysis of N-Linked Glycoproteins	60:93	Deglycosylation by the Acidic Glycosidase PNGase H+ Enables Analysis of N-Linked Glycoproteins by Hydrogen/Deuterium Exchange Mass Spectrometry.					
32955262	1	64	theme	mass	186:189	arg1	HDX-MS					205:210	HDX-MS	205:210	HDX-MS	205:210	Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) has become an important method to study the structural dynamics of proteins.					
32955262	1	64	theme	mass	186:189	arg1	spectrometry					191:202	mass spectrometry	186:202	mass spectrometry (HDX-MS)	186:211	Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) has become an important method to study the structural dynamics of proteins.					
32955262	0	65	theme	Hydrogen/Deuterium	98:115	arg1	Spectrometry					131:142	Hydrogen/Deuterium Exchange Mass Spectrometry	98:142	Hydrogen/Deuterium Exchange Mass Spectrometry	98:142	Deglycosylation by the Acidic Glycosidase PNGase H+ Enables Analysis of N-Linked Glycoproteins by Hydrogen/Deuterium Exchange Mass Spectrometry.					
32955262	2	66	theme	traditional	342:352	arg1	workflow					361:368	the traditional HDX-MS workflow	338:368	the traditional HDX-MS workflow for determining the deuterium uptake of the protein segments that contain the glycan	338:453	However, glycoproteins represent a challenge to the traditional HDX-MS workflow for determining the deuterium uptake of the protein segments that contain the glycan.					
32955262	0	67	theme	Mass	126:129	arg1	Spectrometry					131:142	Hydrogen/Deuterium Exchange Mass Spectrometry	98:142	Hydrogen/Deuterium Exchange Mass Spectrometry	98:142	Deglycosylation by the Acidic Glycosidase PNGase H+ Enables Analysis of N-Linked Glycoproteins by Hydrogen/Deuterium Exchange Mass Spectrometry.					
32955262	2	68	theme	protein	414:420	arg1	segments					422:429	the protein segments	410:429	the protein segments that contain the glycan	410:453	However, glycoproteins represent a challenge to the traditional HDX-MS workflow for determining the deuterium uptake of the protein segments that contain the glycan.					
32955262	7	69	theme	trastuzumab	1197:1207	arg1	analysis					1143:1150	HDX-MS analysis	1136:1150	HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ)	1136:1212	We demonstrate that PNGase A and PNGase H+ are capable of similar deglycosylation performance during HDX-MS analysis of α1-antichymotrypsin and the IgG1 antibody trastuzumab (TZ).					
32955262	9	70	gly	glycoproteins	1569:1581	arg1	glycoproteins					1569:1581	glycoproteins	1569:1581	glycoproteins containing both glycosylations and disulfide bonds	1569:1632	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	8	71	theme	commercial	1373:1382	arg1	availability					1384:1395	commercial availability	1373:1395	commercial availability	1373:1395	However, PNGase H+ provides broader specificity and greater tolerance to the disulfide-bond reducing agent TCEP, while PNGase A offers advantages in terms of commercial availability and purity.					
32955262	0	72	theme	Acidic	23:28	arg1	H+					49:50	the Acidic Glycosidase PNGase H+	19:50	the Acidic Glycosidase PNGase H+	19:50	Deglycosylation by the Acidic Glycosidase PNGase H+ Enables Analysis of N-Linked Glycoproteins by Hydrogen/Deuterium Exchange Mass Spectrometry.					
32955262	9	73	theme	disulfide	1618:1626	arg1	bonds					1628:1632	disulfide bonds	1618:1632	disulfide bonds	1618:1632	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	4	74	theme	HDX-MS	757:762	arg1	analysis					764:771	HDX-MS analysis	757:771	HDX-MS analysis of glycoproteins	757:788	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	0	75	theme	PNGase	42:47	arg1	H+					49:50	the Acidic Glycosidase PNGase H+	19:50	the Acidic Glycosidase PNGase H+	19:50	Deglycosylation by the Acidic Glycosidase PNGase H+ Enables Analysis of N-Linked Glycoproteins by Hydrogen/Deuterium Exchange Mass Spectrometry.					
32955262	1	76	theme	important	227:235	arg1	method					237:242	an important method	224:242	an important method to study the structural dynamics of proteins	224:287	Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) has become an important method to study the structural dynamics of proteins.					
32955262	9	77	theme	conformational	1490:1503	arg1	analysis					1505:1512	conformational analysis	1490:1512	conformational analysis of glycoproteins	1490:1529	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	4	78	gly	glycosylated	728:739	arg1	peptides					741:748	N-linked glycosylated peptides	719:748	N-linked glycosylated peptides	719:748	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	6	79	gly	glycosylated	979:990	arg1	regions					992:998	all five glycosylated regions	970:998	all five glycosylated regions of the serpin α1-antichymotrypsin	970:1032	When used in an HDX-MS workflow, PNGase H+ allowed the extraction of HDX data from all five glycosylated regions of the serpin α1-antichymotrypsin.					
32955262	3	80	theme	A	524:524	arg1	utility					490:496	the utility	486:496	the utility of the glycosidase PNGase A to enable HDX-MS analysis of N-glycosylated protein regions	486:584	We have recently demonstrated the utility of the glycosidase PNGase A to enable HDX-MS analysis of N-glycosylated protein regions.					
32955262	4	81	contain	has	665:667	arg2	optimum					674:680	a pH optimum	669:680	a pH optimum at 2.6	669:687	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	4	81	contain	has	665:667	arg1	H+					655:656	the acidic glycosidase PNGase H+	625:656	the acidic glycosidase PNGase H+	625:656	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	3	82	theme	glycosidase	505:515	arg1	A					524:524	the glycosidase PNGase A	501:524	the glycosidase PNGase A	501:524	We have recently demonstrated the utility of the glycosidase PNGase A to enable HDX-MS analysis of N-glycosylated protein regions.					
32955262	4	83	theme	glycosidase	636:646	arg1	H+					655:656	the acidic glycosidase PNGase H+	625:656	the acidic glycosidase PNGase H+	625:656	Here, we have investigated the use of the acidic glycosidase PNGase H+, which has a pH optimum at 2.6, to efficiently deglycosylate N-linked glycosylated peptides during HDX-MS analysis of glycoproteins.					
32955262	9	84	contain	containing	1583:1592	arg2	bonds					1628:1632	disulfide bonds	1618:1632	disulfide bonds	1618:1632	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	9	84	contain	containing	1583:1592	arg1	glycoproteins					1569:1581	glycoproteins	1569:1581	glycoproteins containing both glycosylations and disulfide bonds	1569:1632	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	9	84	contain	containing	1583:1592	arg2	glycosylations					1599:1612	glycosylations	1599:1612	glycosylations	1599:1612	Overall, our findings demonstrate the unique features of PNGase H+ for improving conformational analysis of glycoproteins by HDX-MS, in particular, challenging glycoproteins containing both glycosylations and disulfide bonds.					
32955262	2	85	theme	deuterium	390:398	arg1	uptake					400:405	the deuterium uptake	386:405	the deuterium uptake of the protein segments that contain the glycan	386:453	However, glycoproteins represent a challenge to the traditional HDX-MS workflow for determining the deuterium uptake of the protein segments that contain the glycan.					
32955262	1	86	theme	structural	257:266	arg1	dynamics					268:275	the structural dynamics	253:275	the structural dynamics of proteins	253:287	Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) has become an important method to study the structural dynamics of proteins.					
33712130	0	0	theme	templated	85:93	arg1	assembly					95:102	templated assembly	85:102	templated assembly of silver nanoparticles into biocidal composite material	85:159	Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material.					
33712130	4	1	theme	Synthetic	721:729	arg1	oligomers					741:749	Synthetic cellulose oligomers	721:749	Synthetic cellulose oligomers	721:749	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33712130	2	2	theme	templating	408:417	arg1	materials					434:442	templating such composite materials	408:442	templating such composite materials	408:442	Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials.					
33712130	5	3	theme	silver/g	1099:1106	arg1	thiol-cellulose					1108:1122	∼2.2 g silver/g thiol-cellulose	1092:1122	∼2.2 g silver/g thiol-cellulose	1092:1122	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	5	3	theme	silver/g	1099:1106	arg1	efficiency					1080:1089	high loading efficiency	1067:1089	high loading efficiency (∼2.2 g silver/g thiol-cellulose)	1067:1123	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	5	4	theme	high	1067:1070	arg1	thiol-cellulose					1108:1122	∼2.2 g silver/g thiol-cellulose	1092:1122	∼2.2 g silver/g thiol-cellulose	1092:1122	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	5	4	theme	high	1067:1070	arg1	efficiency					1080:1089	high loading efficiency	1067:1089	high loading efficiency (∼2.2 g silver/g thiol-cellulose)	1067:1123	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	5	5	theme	g	1097:1097	arg1	thiol-cellulose					1108:1122	∼2.2 g silver/g thiol-cellulose	1092:1122	∼2.2 g silver/g thiol-cellulose	1092:1122	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	5	5	theme	g	1097:1097	arg1	efficiency					1080:1089	high loading efficiency	1067:1089	high loading efficiency (∼2.2 g silver/g thiol-cellulose)	1067:1123	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	3	6	dep	1-phosphate	699:709	arg1	mM					716:717	100 mM	712:717	100 mM	712:717	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	4	7	theme	nm	918:919	arg1	thickness					901:909	thickness	901:909	thickness of 5-7 nm	901:919	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33712130	6	8	theme	surface-patterned	1187:1203	arg1	nanoparticles					1205:1217	surface-patterned nanoparticles	1187:1217	surface-patterned nanoparticles	1187:1217	Examined against Escherichia coli and Staphylococcus aureus, surface-patterned nanoparticles show excellent biocidal activity.					
33712130	2	9	theme	tunable	334:340	arg1	morphology					352:361	tunable nanoscale morphology	334:361	tunable nanoscale morphology	334:361	Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials.					
33712130	3	10	theme	polymerization	619:632	arg1	degree					609:614	a degree	607:614	a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM)	607:718	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	7	11	theme	Bottom-up	1253:1261	arg1	approach					1263:1270	Bottom-up approach	1253:1270	Bottom-up approach by chemical design to a functional cellulose nanocomposite	1253:1329	Bottom-up approach by chemical design to a functional cellulose nanocomposite is presented.					
33712130	3	12	from	substrate	676:684	arg1	yield					649:653	a yield	647:653	a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM)	647:718	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	8	13	theme	cellulose	1428:1436	arg1	materials					1438:1446	top-down produced cellulose materials	1410:1446	top-down produced cellulose materials	1410:1446	Synthetic thiol-containing nanocellulose can expand the scope of top-down produced cellulose materials.					
33712130	4	14	theme	cellulose	798:806	arg1	crystalline					785:795	crystalline	785:795	highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies	770:893	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33712130	4	14	theme	cellulose	798:806	arg1	allomorph					808:816	cellulose allomorph II	798:819	cellulose allomorph II	798:819	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33712130	5	15	theme	Silver	922:927	arg1	nanoparticles					929:941	Silver nanoparticles	922:941	Silver nanoparticles	922:941	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	0	16	theme	nanoparticles	114:126	arg1	assembly					95:102	templated assembly	85:102	templated assembly of silver nanoparticles into biocidal composite material	85:159	Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material.					
33712130	8	17	theme	Synthetic	1345:1353	arg1	nanocellulose					1372:1384	Synthetic thiol-containing nanocellulose	1345:1384	Synthetic thiol-containing nanocellulose	1345:1384	Synthetic thiol-containing nanocellulose can expand the scope of top-down produced cellulose materials.					
33712130	5	18	theme	thiol-modified	1010:1023	arg1	cellulose					1025:1033	the thiol-modified cellulose	1006:1033	the thiol-modified cellulose	1006:1033	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	1	19	theme	important	198:206	arg1	materials					219:227	important functional materials	198:227	important functional materials	198:227	Nanoparticle-polymer composites are important functional materials but structural control of their assembly is challenging.					
33712130	1	19	theme	important	198:206	arg1	composites					183:192	Nanoparticle-polymer composites	162:192	Nanoparticle-polymer composites	162:192	Nanoparticle-polymer composites are important functional materials but structural control of their assembly is challenging.					
33712130	0	20	theme	silver	107:112	arg1	nanoparticles					114:126	silver nanoparticles	107:126	silver nanoparticles	107:126	Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material.					
33712130	3	21	theme	bottom-up	459:467	arg1	synthesis					469:477	bottom-up synthesis	459:477	bottom-up synthesis of reducing end thiol-modified cellulose chains	459:525	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	2	22	theme	internal	311:318	arg1	structure					320:328	crystalline internal structure	299:328	crystalline internal structure	299:328	Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials.					
33712130	3	23	from	%	661:661	arg1	substrate					676:684	the donor substrate	666:684	the donor substrate (α-d-glucose 1-phosphate, 100 mM)	666:718	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	3	23	from	%	661:661	arg1	1-phosphate					699:709	α-d-glucose 1-phosphate	687:709	α-d-glucose 1-phosphate	687:709	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	1	24	theme	functional	208:217	arg1	materials					219:227	important functional materials	198:227	important functional materials	198:227	Nanoparticle-polymer composites are important functional materials but structural control of their assembly is challenging.					
33712130	1	24	theme	functional	208:217	arg1	composites					183:192	Nanoparticle-polymer composites	162:192	Nanoparticle-polymer composites	162:192	Nanoparticle-polymer composites are important functional materials but structural control of their assembly is challenging.					
33712130	0	25	theme	end	9:11	arg1	synthesis					63:71	end thiol-modified nanocellulose: Bottom-up enzymatic synthesis	9:71	end thiol-modified nanocellulose: Bottom-up enzymatic synthesis	9:71	Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material.					
33712130	7	26	theme	cellulose	1307:1315	arg1	nanocomposite					1317:1329	a functional cellulose nanocomposite	1294:1329	a functional cellulose nanocomposite	1294:1329	Bottom-up approach by chemical design to a functional cellulose nanocomposite is presented.					
33712130	4	27	theme	ordered	777:783	arg1	material					822:829	highly ordered crystalline (cellulose allomorph II) material	770:829	highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies	770:893	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33712130	5	28	theme	loading	1072:1078	arg1	thiol-cellulose					1108:1122	∼2.2 g silver/g thiol-cellulose	1092:1122	∼2.2 g silver/g thiol-cellulose	1092:1122	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	5	28	theme	loading	1072:1078	arg1	efficiency					1080:1089	high loading efficiency	1067:1089	high loading efficiency (∼2.2 g silver/g thiol-cellulose)	1067:1123	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	3	29	theme	∼8	637:638	arg1	polymerization					619:632	polymerization	619:632	polymerization of ∼8	619:638	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	3	30	theme	reducing	482:489	arg1	chains					520:525	reducing end thiol-modified cellulose chains	482:525	reducing end thiol-modified cellulose chains	482:525	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	0	31	theme	nanocellulose	28:40	arg1	synthesis					63:71	end thiol-modified nanocellulose: Bottom-up enzymatic synthesis	9:71	end thiol-modified nanocellulose: Bottom-up enzymatic synthesis	9:71	Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material.					
33712130	0	32	theme	biocidal	133:140	arg1	material					152:159	biocidal composite material	133:159	biocidal composite material	133:159	Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material.					
33712130	3	33	gly	β-1,4-glycosylation	553:571	arg1	1-thio-β-d-glucose					576:593	1-thio-β-d-glucose	576:593	1-thio-β-d-glucose (10 mM)	576:601	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	3	33	gly	β-1,4-glycosylation	553:571	arg1	mM					599:600	10 mM	596:600	10 mM	596:600	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	1	34	theme	structural	233:242	arg1	control					244:250	structural control	233:250	structural control of their assembly	233:268	Nanoparticle-polymer composites are important functional materials but structural control of their assembly is challenging.					
33712130	0	35	theme	thiol-modified	13:26	arg1	synthesis					63:71	end thiol-modified nanocellulose: Bottom-up enzymatic synthesis	9:71	end thiol-modified nanocellulose: Bottom-up enzymatic synthesis	9:71	Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material.					
33712130	8	36	theme	materials	1438:1446	arg1	scope					1401:1405	the scope	1397:1405	the scope of top-down produced cellulose materials	1397:1446	Synthetic thiol-containing nanocellulose can expand the scope of top-down produced cellulose materials.					
33712130	2	37	theme	nanoscale	342:350	arg1	morphology					352:361	tunable nanoscale morphology	334:361	tunable nanoscale morphology	334:361	Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials.					
33712130	3	38	theme	iterative	530:538	arg1	β-1,4-glycosylation					553:571	iterative bi-enzymatic β-1,4-glycosylation	530:571	iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM)	530:601	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	0	39	theme	Bottom-up	43:51	arg1	synthesis					63:71	end thiol-modified nanocellulose: Bottom-up enzymatic synthesis	9:71	end thiol-modified nanocellulose: Bottom-up enzymatic synthesis	9:71	Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material.					
33712130	3	40	theme	1-thio-β-d-glucose	576:593	arg1	β-1,4-glycosylation					553:571	iterative bi-enzymatic β-1,4-glycosylation	530:571	iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM)	530:601	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	7	41	theme	functional	1296:1305	arg1	nanocomposite					1317:1329	a functional cellulose nanocomposite	1294:1329	a functional cellulose nanocomposite	1294:1329	Bottom-up approach by chemical design to a functional cellulose nanocomposite is presented.					
33712130	3	42	from	yield	649:653	arg1	substrate					676:684	the donor substrate	666:684	the donor substrate (α-d-glucose 1-phosphate, 100 mM)	666:718	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	3	42	from	yield	649:653	arg1	1-phosphate					699:709	α-d-glucose 1-phosphate	687:709	α-d-glucose 1-phosphate	687:709	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	3	43	theme	cellulose	510:518	arg1	chains					520:525	reducing end thiol-modified cellulose chains	482:525	reducing end thiol-modified cellulose chains	482:525	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	3	44	theme	end	491:493	arg1	chains					520:525	reducing end thiol-modified cellulose chains	482:525	reducing end thiol-modified cellulose chains	482:525	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	0	45	theme	composite	142:150	arg1	material					152:159	biocidal composite material	133:159	biocidal composite material	133:159	Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material.					
33712130	5	46	theme	≥	1056:1056	arg1	yield					1049:1053	excellent yield	1039:1053	excellent yield (≥ 95%)	1039:1061	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	5	46	theme	≥	1056:1056	arg1	%					1060:1060	≥ 95%	1056:1060	≥ 95%	1056:1060	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	3	47	theme	chains	520:525	arg1	synthesis					469:477	bottom-up synthesis	459:477	bottom-up synthesis of reducing end thiol-modified cellulose chains	459:525	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	8	48	theme	produced	1419:1426	arg1	materials					1438:1446	top-down produced cellulose materials	1410:1446	top-down produced cellulose materials	1410:1446	Synthetic thiol-containing nanocellulose can expand the scope of top-down produced cellulose materials.					
33712130	3	49	theme	donor	670:674	arg1	substrate					676:684	the donor substrate	666:684	the donor substrate (α-d-glucose 1-phosphate, 100 mM)	666:718	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	3	49	theme	donor	670:674	arg1	1-phosphate					699:709	α-d-glucose 1-phosphate	687:709	α-d-glucose 1-phosphate	687:709	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	4	50	theme	thin	862:865	arg1	morphologies					882:893	long (micrometers) and thin nanosheet-like morphologies	839:893	long (micrometers) and thin nanosheet-like morphologies	839:893	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33712130	8	51	theme	thiol-containing	1355:1370	arg1	nanocellulose					1372:1384	Synthetic thiol-containing nanocellulose	1345:1384	Synthetic thiol-containing nanocellulose	1345:1384	Synthetic thiol-containing nanocellulose can expand the scope of top-down produced cellulose materials.					
33712130	0	52	theme	enzymatic	53:61	arg1	synthesis					63:71	end thiol-modified nanocellulose: Bottom-up enzymatic synthesis	9:71	end thiol-modified nanocellulose: Bottom-up enzymatic synthesis	9:71	Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material.					
33712130	4	53	theme	nanosheet-like	867:880	arg1	morphologies					882:893	long (micrometers) and thin nanosheet-like morphologies	839:893	long (micrometers) and thin nanosheet-like morphologies	839:893	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33712130	2	54	theme	polymer	387:393	arg1	scaffold					395:402	promising polymer scaffold	377:402	promising polymer scaffold for templating such composite materials	377:442	Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials.					
33712130	6	55	theme	biocidal	1234:1241	arg1	activity					1243:1250	excellent biocidal activity	1224:1250	excellent biocidal activity	1224:1250	Examined against Escherichia coli and Staphylococcus aureus, surface-patterned nanoparticles show excellent biocidal activity.					
33712130	3	56	theme	%	661:661	arg1	yield					649:653	a yield	647:653	a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM)	647:718	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	2	57	theme	crystalline	299:309	arg1	structure					320:328	crystalline internal structure	299:328	crystalline internal structure	299:328	Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials.					
33712130	2	58	theme	promising	377:385	arg1	scaffold					395:402	promising polymer scaffold	377:402	promising polymer scaffold for templating such composite materials	377:442	Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials.					
33712130	1	59	theme	assembly	261:268	arg1	control					244:250	structural control	233:250	structural control of their assembly	233:268	Nanoparticle-polymer composites are important functional materials but structural control of their assembly is challenging.					
33712130	5	60	theme	cellulose	1025:1033	arg1	surface					995:1001	the surface	991:1001	the surface of the thiol-modified cellulose	991:1033	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	3	61	theme	α-d-glucose	687:697	arg1	substrate					676:684	the donor substrate	666:684	the donor substrate (α-d-glucose 1-phosphate, 100 mM)	666:718	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	3	61	theme	α-d-glucose	687:697	arg1	1-phosphate					699:709	α-d-glucose 1-phosphate	687:709	α-d-glucose 1-phosphate	687:709	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	6	62	theme	excellent	1224:1232	arg1	activity					1243:1250	excellent biocidal activity	1224:1250	excellent biocidal activity	1224:1250	Examined against Escherichia coli and Staphylococcus aureus, surface-patterned nanoparticles show excellent biocidal activity.					
33712130	4	63	theme	crystalline	785:795	arg1	material					822:829	highly ordered crystalline (cellulose allomorph II) material	770:829	highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies	770:893	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33712130	1	64	theme	Nanoparticle-polymer	162:181	arg1	materials					219:227	important functional materials	198:227	important functional materials	198:227	Nanoparticle-polymer composites are important functional materials but structural control of their assembly is challenging.					
33712130	1	64	theme	Nanoparticle-polymer	162:181	arg1	composites					183:192	Nanoparticle-polymer composites	162:192	Nanoparticle-polymer composites	162:192	Nanoparticle-polymer composites are important functional materials but structural control of their assembly is challenging.					
33712130	8	65	theme	top-down	1410:1417	arg1	materials					1438:1446	top-down produced cellulose materials	1410:1446	top-down produced cellulose materials	1410:1446	Synthetic thiol-containing nanocellulose can expand the scope of top-down produced cellulose materials.					
33712130	3	66	theme	bi-enzymatic	540:551	arg1	β-1,4-glycosylation					553:571	iterative bi-enzymatic β-1,4-glycosylation	530:571	iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM)	530:601	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	7	67	theme	chemical	1275:1282	arg1	design					1284:1289	chemical design	1275:1289	chemical design	1275:1289	Bottom-up approach by chemical design to a functional cellulose nanocomposite is presented.					
33712130	3	68	from	degree	609:614	arg1	yield					649:653	a yield	647:653	a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM)	647:718	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	5	69	theme	excellent	1039:1047	arg1	yield					1049:1053	excellent yield	1039:1053	excellent yield (≥ 95%)	1039:1061	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	5	69	theme	excellent	1039:1047	arg1	%					1060:1060	≥ 95%	1056:1060	≥ 95%	1056:1060	Silver nanoparticles were attached selectively and well dispersed on the surface of the thiol-modified cellulose, in excellent yield (≥ 95%) and high loading efficiency (∼2.2 g silver/g thiol-cellulose).					
33712130	4	70	dep	long	839:842	arg1	micrometers					845:855	micrometers	845:855	micrometers	845:855	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33712130	2	71	theme	composite	424:432	arg1	materials					434:442	templating such composite materials	408:442	templating such composite materials	408:442	Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials.					
33712130	3	72	theme	thiol-modified	495:508	arg1	chains					520:525	reducing end thiol-modified cellulose chains	482:525	reducing end thiol-modified cellulose chains	482:525	Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-d-glucose (10 mM), to a degree of polymerization of ∼8 and in a yield of ∼41% on the donor substrate (α-d-glucose 1-phosphate, 100 mM).					
33712130	4	73	theme	long	839:842	arg1	morphologies					882:893	long (micrometers) and thin nanosheet-like morphologies	839:893	long (micrometers) and thin nanosheet-like morphologies	839:893	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33712130	2	74	theme	such	419:422	arg1	materials					434:442	templating such composite materials	408:442	templating such composite materials	408:442	Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials.					
33712130	4	75	theme	cellulose	731:739	arg1	oligomers					741:749	Synthetic cellulose oligomers	721:749	Synthetic cellulose oligomers	721:749	Synthetic cellulose oligomers self-assemble into highly ordered crystalline (cellulose allomorph II) material showing long (micrometers) and thin nanosheet-like morphologies, with thickness of 5-7 nm.					
33188812	4	0	theme	different	880:888	arg1	techniques					897:906	different drying techniques	880:906	different drying techniques	880:906	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	1	1	from	characteristics	408:422	arg1	dandelion					466:474	dandelion leaves	466:481	dandelion leaves (DLPs)	466:488	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	1	from	characteristics	408:422	arg1	DLPs					484:487	DLPs	484:487	DLPs	484:487	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	2	2	theme	drying	571:576	arg1	techniques					578:587	abovementioned drying techniques	556:587	abovementioned drying techniques	556:587	Five different DLPs were obtained from dandelion leaves dried by abovementioned drying techniques.					
33188812	5	3	theme	scavenging	1207:1216	arg1	activities					1218:1227	NO radical scavenging activities	1196:1227	NO radical scavenging activities	1196:1227	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	1	4	theme	frequency	264:272	arg1	drying					274:279	radio frequency drying	258:279	radio frequency drying	258:279	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	4	theme	frequency	264:272	arg1	techniques					246:255	innovative drying techniques	228:255	innovative drying techniques (radio frequency drying and microwave drying)	228:301	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	5	5	dep	in	1286:1287	arg1	vitro					1289:1293	vitro	1289:1293	vitro	1289:1293	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	5	6	theme	radical	1199:1205	arg1	activities					1218:1227	NO radical scavenging activities	1196:1227	NO radical scavenging activities	1196:1227	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	1	7	theme	polysaccharides	445:459	arg1	bioactivities					428:440	bioactivities	428:440	bioactivities	428:440	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	7	theme	polysaccharides	445:459	arg1	characteristics					408:422	structural characteristics	397:422	structural characteristics	397:422	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	0	8	theme	structural	114:123	arg1	characteristics					125:139	their structural characteristics	108:139	their structural characteristics	108:139	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.					
33188812	1	9	theme	air	378:380	arg1	drying					382:387	hot air drying	374:387	hot air drying	374:387	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	9	theme	air	378:380	arg1	techniques					246:255	innovative drying techniques	228:255	innovative drying techniques (radio frequency drying and microwave drying)	228:301	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	4	10	theme	noticeable	912:921	arg1	variations					923:932	noticeable variations	912:932	noticeable variations	912:932	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	11	from	contents	827:834	arg1	DLPs					863:866	DLPs	863:866	DLPs obtained by different drying techniques	863:906	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	5	12	theme	in	1286:1287	arg1	activity					1306:1313	obvious in vitro inhibitory activity	1278:1313	obvious in vitro inhibitory activity	1278:1313	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	1	13	dep	characteristics	408:422	arg1	the					393:395	the	393:395	the	393:395	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	4	14	theme	glycosidic	996:1005	arg1	linkages					1007:1014	the major glycosidic linkages	986:1014	the major glycosidic linkages in DLPs	986:1022	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	5	15	theme	inhibitory	1295:1304	arg1	activity					1306:1313	obvious in vitro inhibitory activity	1278:1313	obvious in vitro inhibitory activity	1278:1313	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	4	16	from	monosaccharides	966:980	arg1	DLPs					1019:1022	DLPs	1019:1022	DLPs	1019:1022	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	1	17	theme	techniques	326:335	arg1	impacts					217:223	the impacts	213:223	the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs)	213:488	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	18	dep	dandelion	466:474	arg1	leaves					476:481	leaves	476:481	leaves	476:481	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	0	19	from	dandelion	21:29	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.	0:166	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.					
33188812	4	20	theme	molecular	712:720	arg1	weights					722:728	The molecular weights	708:728	The molecular weights	708:728	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	6	21	theme	frequency	1380:1388	arg1	drying					1390:1395	the radio frequency drying	1370:1395	the radio frequency drying	1370:1395	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	6	21	theme	frequency	1380:1388	arg1	technique					1431:1439	a potential drying technique	1412:1439	a potential drying technique	1412:1439	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	1	22	theme	vacuum	338:343	arg1	drying					345:350	vacuum drying	338:350	vacuum drying	338:350	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	22	theme	vacuum	338:343	arg1	techniques					246:255	innovative drying techniques	228:255	innovative drying techniques (radio frequency drying and microwave drying)	228:301	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	4	23	from	contents	797:804	arg1	DLPs					863:866	DLPs	863:866	DLPs obtained by different drying techniques	863:906	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	24	theme	monosaccharides	966:980	arg1	similar					1029:1035	similar	1029:1035	similar	1029:1035	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	24	theme	monosaccharides	966:980	arg1	types					945:949	the types	941:949	the types of constituent monosaccharides and the major glycosidic linkages in DLPs	941:1022	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	25	theme	monosaccharide	781:794	arg1	ratios					759:764	molar ratios	753:764	molar ratios of constituent monosaccharide	753:794	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	25	theme	monosaccharide	781:794	arg1	contents					827:834	contents	827:834	contents	827:834	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	25	theme	monosaccharide	781:794	arg1	viscosities					740:750	apparent viscosities	731:750	apparent viscosities	731:750	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	25	theme	monosaccharide	781:794	arg1	weights					722:728	The molecular weights	708:728	The molecular weights	708:728	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	25	theme	monosaccharide	781:794	arg1	contents					797:804	contents	797:804	contents of uronic acids	797:820	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	0	26	theme	drying	87:92	arg1	techniques					94:103	innovative drying techniques	76:103	innovative drying techniques	76:103	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.					
33188812	2	27	theme	different	496:504	arg1	DLPs					506:509	Five different DLPs	491:509	Five different DLPs	491:509	Five different DLPs were obtained from dandelion leaves dried by abovementioned drying techniques.					
33188812	5	28	theme	frequency	1117:1125	arg1	drying					1127:1132	the radio frequency drying	1107:1132	the radio frequency drying	1107:1132	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	6	29	from	applications	1468:1479	arg1	industries					1517:1526	the functional food and medicine industries	1484:1526	the functional food and medicine industries	1484:1526	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	4	30	contain	had	908:910	arg1	viscosities					740:750	apparent viscosities	731:750	apparent viscosities	731:750	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	30	contain	had	908:910	arg2	variations					923:932	noticeable variations	912:932	noticeable variations	912:932	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	30	contain	had	908:910	arg1	contents					797:804	contents	797:804	contents of uronic acids	797:820	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	30	contain	had	908:910	arg1	contents					827:834	contents	827:834	contents	827:834	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	30	contain	had	908:910	arg1	ratios					759:764	molar ratios	753:764	molar ratios of constituent monosaccharide	753:794	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	30	contain	had	908:910	arg1	weights					722:728	The molecular weights	708:728	The molecular weights	708:728	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	31	theme	molar	753:757	arg1	ratios					759:764	molar ratios	753:764	molar ratios of constituent monosaccharide	753:794	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	6	32	theme	drying	1424:1429	arg1	technique					1431:1439	a potential drying technique	1412:1439	a potential drying technique	1412:1439	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	6	32	theme	drying	1424:1429	arg1	drying					1390:1395	the radio frequency drying	1370:1395	the radio frequency drying	1370:1395	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	1	33	theme	microwave	285:293	arg1	drying					295:300	microwave drying	285:300	microwave drying	285:300	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	33	theme	microwave	285:293	arg1	techniques					246:255	innovative drying techniques	228:255	innovative drying techniques (radio frequency drying and microwave drying)	228:301	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	6	34	used	used	1404:1407	arg2	drying					1390:1395	the radio frequency drying	1370:1395	the radio frequency drying	1370:1395	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	6	34	used	used	1404:1407	arg2	technique					1431:1439	a potential drying technique	1412:1439	a potential drying technique	1412:1439	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	3	35	theme	DLPs	662:665	arg1	bioactivities					645:657	bioactivities	645:657	bioactivities	645:657	Results showed that the structural characteristics and bioactivities of DLPs varied with different drying techniques.					
33188812	3	35	theme	DLPs	662:665	arg1	characteristics					625:639	the structural characteristics	610:639	the structural characteristics	610:639	Results showed that the structural characteristics and bioactivities of DLPs varied with different drying techniques.					
33188812	4	36	theme	bonded	839:844	arg1	polyphenolics					846:858	bonded polyphenolics	839:858	bonded polyphenolics	839:858	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	1	37	theme	traditional	307:317	arg1	techniques					326:335	traditional drying techniques	307:335	traditional drying techniques	307:335	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	3	38	theme	drying	689:694	arg1	techniques					696:705	different drying techniques	679:705	different drying techniques	679:705	Results showed that the structural characteristics and bioactivities of DLPs varied with different drying techniques.					
33188812	4	39	theme	uronic	809:814	arg1	acids					816:820	uronic acids	809:820	uronic acids	809:820	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	6	40	theme	food	1499:1502	arg1	industries					1517:1526	the functional food and medicine industries	1484:1526	the functional food and medicine industries	1484:1526	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	0	41	dep	dandelion	21:29	arg1	mongolicum					42:51	Taraxacum mongolicum	32:51	Taraxacum mongolicum	32:51	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.					
33188812	0	41	dep	dandelion	21:29	arg1	leaves					54:59	leaves	54:59	leaves	54:59	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.					
33188812	4	42	from	weights	722:728	arg1	DLPs					863:866	DLPs	863:866	DLPs obtained by different drying techniques	863:906	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	5	43	theme	in	1241:1242	arg1	activity					1264:1271	excellent in vitro antiglycation activity	1231:1271	excellent in vitro antiglycation activity	1231:1271	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	0	44	dep	Polysaccharides	0:14	arg1	Insights					62:69	Insights	62:69	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.	0:166	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.					
33188812	1	45	theme	techniques	246:255	arg1	impacts					217:223	the impacts	213:223	the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs)	213:488	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	46	theme	radio	258:262	arg1	drying					274:279	radio frequency drying	258:279	radio frequency drying	258:279	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	46	theme	radio	258:262	arg1	techniques					246:255	innovative drying techniques	228:255	innovative drying techniques (radio frequency drying and microwave drying)	228:301	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	4	47	theme	drying	890:895	arg1	techniques					897:906	different drying techniques	880:906	different drying techniques	880:906	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	2	48	dep	dandelion	530:538	arg1	leaves					540:545	leaves	540:545	leaves	540:545	Five different DLPs were obtained from dandelion leaves dried by abovementioned drying techniques.					
33188812	4	49	theme	polyphenolics	846:858	arg1	ratios					759:764	molar ratios	753:764	molar ratios of constituent monosaccharide	753:794	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	49	theme	polyphenolics	846:858	arg1	contents					827:834	contents	827:834	contents	827:834	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	49	theme	polyphenolics	846:858	arg1	viscosities					740:750	apparent viscosities	731:750	apparent viscosities	731:750	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	49	theme	polyphenolics	846:858	arg1	weights					722:728	The molecular weights	708:728	The molecular weights	708:728	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	49	theme	polyphenolics	846:858	arg1	contents					797:804	contents	797:804	contents of uronic acids	797:820	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	1	50	theme	study	184:188	arg1	aim					172:174	The aim	168:174	The aim of this study	168:188	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	5	51	theme	remarkable	1145:1154	arg1	ABTS					1180:1183	ABTS	1180:1183	ABTS	1180:1183	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	5	51	theme	remarkable	1145:1154	arg1	activities					1168:1177	remarkable antioxidant activities	1145:1177	remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities)	1145:1228	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	5	51	theme	remarkable	1145:1154	arg1	DPPH					1186:1189	DPPH	1186:1189	DPPH	1186:1189	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	5	52	dep	in	1241:1242	arg1	vitro					1244:1248	vitro	1244:1248	vitro	1244:1248	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	0	53	theme	biological	145:154	arg1	activities					156:165	biological activities	145:165	biological activities	145:165	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.					
33188812	1	54	theme	hot	374:376	arg1	drying					382:387	hot air drying	374:387	hot air drying	374:387	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	54	theme	hot	374:376	arg1	techniques					246:255	innovative drying techniques	228:255	innovative drying techniques (radio frequency drying and microwave drying)	228:301	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	6	55	theme	medicine	1508:1515	arg1	industries					1517:1526	the functional food and medicine industries	1484:1526	the functional food and medicine industries	1484:1526	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	0	56	from	Insights	62:69	arg1	characteristics					125:139	their structural characteristics	108:139	their structural characteristics	108:139	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.					
33188812	0	56	from	Insights	62:69	arg1	activities					156:165	biological activities	145:165	biological activities	145:165	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.					
33188812	1	57	theme	drying	319:324	arg1	techniques					326:335	traditional drying techniques	307:335	traditional drying techniques	307:335	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	2	58	theme	abovementioned	556:569	arg1	techniques					578:587	abovementioned drying techniques	556:587	abovementioned drying techniques	556:587	Five different DLPs were obtained from dandelion leaves dried by abovementioned drying techniques.					
33188812	5	59	theme	obvious	1278:1284	arg1	activity					1306:1313	obvious in vitro inhibitory activity	1278:1313	obvious in vitro inhibitory activity	1278:1313	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	1	60	from	impacts	217:223	arg1	bioactivities					428:440	bioactivities	428:440	bioactivities	428:440	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	60	from	impacts	217:223	arg1	characteristics					408:422	structural characteristics	397:422	structural characteristics	397:422	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	3	61	theme	structural	614:623	arg1	characteristics					625:639	the structural characteristics	610:639	the structural characteristics	610:639	Results showed that the structural characteristics and bioactivities of DLPs varied with different drying techniques.					
33188812	1	62	dep	techniques	246:255	arg1	drying					382:387	hot air drying	374:387	hot air drying	374:387	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	62	dep	techniques	246:255	arg1	drying					274:279	radio frequency drying	258:279	radio frequency drying	258:279	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	62	dep	techniques	246:255	arg1	drying					362:367	freezing drying	353:367	freezing drying	353:367	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	62	dep	techniques	246:255	arg1	drying					295:300	microwave drying	285:300	microwave drying	285:300	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	62	dep	techniques	246:255	arg1	techniques					246:255	innovative drying techniques	228:255	innovative drying techniques (radio frequency drying and microwave drying)	228:301	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	62	dep	techniques	246:255	arg1	drying					345:350	vacuum drying	338:350	vacuum drying	338:350	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	4	63	theme	apparent	731:738	arg1	viscosities					740:750	apparent viscosities	731:750	apparent viscosities	731:750	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	1	64	theme	structural	397:406	arg1	characteristics					408:422	structural characteristics	397:422	structural characteristics	397:422	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	4	65	theme	major	990:994	arg1	linkages					1007:1014	the major glycosidic linkages	986:1014	the major glycosidic linkages in DLPs	986:1022	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	5	66	theme	antiglycation	1250:1262	arg1	activity					1264:1271	excellent in vitro antiglycation activity	1231:1271	excellent in vitro antiglycation activity	1231:1271	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	0	67	theme	innovative	76:85	arg1	techniques					94:103	innovative drying techniques	76:103	innovative drying techniques	76:103	Polysaccharides from dandelion (Taraxacum mongolicum) leaves: Insights into innovative drying techniques on their structural characteristics and biological activities.					
33188812	4	68	theme	linkages	1007:1014	arg1	similar					1029:1035	similar	1029:1035	similar	1029:1035	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	68	theme	linkages	1007:1014	arg1	types					945:949	the types	941:949	the types of constituent monosaccharides and the major glycosidic linkages in DLPs	941:1022	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	6	69	from	study	1351:1355	arg1	Results					1333:1339	Results	1333:1339	Results from this study	1333:1355	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	6	70	theme	radio	1374:1378	arg1	drying					1390:1395	the radio frequency drying	1370:1395	the radio frequency drying	1370:1395	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	6	70	theme	radio	1374:1378	arg1	technique					1431:1439	a potential drying technique	1412:1439	a potential drying technique	1412:1439	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	5	71	theme	antioxidant	1156:1166	arg1	ABTS					1180:1183	ABTS	1180:1183	ABTS	1180:1183	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	5	71	theme	antioxidant	1156:1166	arg1	activities					1168:1177	remarkable antioxidant activities	1145:1177	remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities)	1145:1228	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	5	71	theme	antioxidant	1156:1166	arg1	DPPH					1186:1189	DPPH	1186:1189	DPPH	1186:1189	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	4	72	theme	constituent	954:964	arg1	monosaccharides					966:980	constituent monosaccharides	954:980	constituent monosaccharides	954:980	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	73	theme	constituent	769:779	arg1	monosaccharide					781:794	constituent monosaccharide	769:794	constituent monosaccharide	769:794	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	1	74	theme	freezing	353:360	arg1	drying					362:367	freezing drying	353:367	freezing drying	353:367	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	74	theme	freezing	353:360	arg1	techniques					246:255	innovative drying techniques	228:255	innovative drying techniques (radio frequency drying and microwave drying)	228:301	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	5	75	dep	activities	1168:1177	arg1	ABTS					1180:1183	ABTS	1180:1183	ABTS	1180:1183	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	5	75	dep	activities	1168:1177	arg1	activities					1218:1227	NO radical scavenging activities	1196:1227	NO radical scavenging activities	1196:1227	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	5	75	dep	activities	1168:1177	arg1	activities					1168:1177	remarkable antioxidant activities	1145:1177	remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities)	1145:1228	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	5	75	dep	activities	1168:1177	arg1	DPPH					1186:1189	DPPH	1186:1189	DPPH	1186:1189	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	5	76	theme	excellent	1231:1239	arg1	activity					1264:1271	excellent in vitro antiglycation activity	1231:1271	excellent in vitro antiglycation activity	1231:1271	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	4	77	from	ratios	759:764	arg1	DLPs					863:866	DLPs	863:866	DLPs obtained by different drying techniques	863:906	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	5	78	theme	radio	1111:1115	arg1	drying					1127:1132	the radio frequency drying	1107:1132	the radio frequency drying	1107:1132	In addition, results showed that DLPs, especially DLP-RF obtained by the radio frequency drying, exhibited remarkable antioxidant activities (ABTS, DPPH, and NO radical scavenging activities), excellent in vitro antiglycation activity, and obvious in vitro inhibitory activity on α-glucosidase.					
33188812	4	79	from	types	945:949	arg1	DLPs					1019:1022	DLPs	1019:1022	DLPs	1019:1022	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	6	80	theme	potential	1414:1422	arg1	technique					1431:1439	a potential drying technique	1412:1439	a potential drying technique	1412:1439	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	6	80	theme	potential	1414:1422	arg1	drying					1390:1395	the radio frequency drying	1370:1395	the radio frequency drying	1370:1395	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	1	81	theme	innovative	228:237	arg1	drying					382:387	hot air drying	374:387	hot air drying	374:387	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	81	theme	innovative	228:237	arg1	drying					274:279	radio frequency drying	258:279	radio frequency drying	258:279	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	81	theme	innovative	228:237	arg1	drying					362:367	freezing drying	353:367	freezing drying	353:367	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	81	theme	innovative	228:237	arg1	drying					295:300	microwave drying	285:300	microwave drying	285:300	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	81	theme	innovative	228:237	arg1	techniques					246:255	innovative drying techniques	228:255	innovative drying techniques (radio frequency drying and microwave drying)	228:301	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	81	theme	innovative	228:237	arg1	drying					345:350	vacuum drying	338:350	vacuum drying	338:350	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	82	from	dandelion	466:474	arg1	bioactivities					428:440	bioactivities	428:440	bioactivities	428:440	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	82	from	dandelion	466:474	arg1	characteristics					408:422	structural characteristics	397:422	structural characteristics	397:422	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	82	from	dandelion	466:474	arg1	polysaccharides					445:459	polysaccharides	445:459	polysaccharides from dandelion leaves (DLPs)	445:488	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	3	83	theme	different	679:687	arg1	techniques					696:705	different drying techniques	679:705	different drying techniques	679:705	Results showed that the structural characteristics and bioactivities of DLPs varied with different drying techniques.					
33188812	4	84	from	viscosities	740:750	arg1	DLPs					863:866	DLPs	863:866	DLPs obtained by different drying techniques	863:906	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	85	from	DLPs	1019:1022	arg1	similar					1029:1035	similar	1029:1035	similar	1029:1035	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	85	from	DLPs	1019:1022	arg1	types					945:949	the types	941:949	the types of constituent monosaccharides and the major glycosidic linkages in DLPs	941:1022	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	6	86	theme	functional	1488:1497	arg1	food					1499:1502	functional food	1488:1502	functional food	1488:1502	Results from this study suggest that the radio frequency drying can be used as a potential drying technique before extracting DLPs for applications in the functional food and medicine industries.					
33188812	4	87	from	linkages	1007:1014	arg1	DLPs					1019:1022	DLPs	1019:1022	DLPs	1019:1022	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	1	88	from	bioactivities	428:440	arg1	dandelion					466:474	dandelion leaves	466:481	dandelion leaves (DLPs)	466:488	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	88	from	bioactivities	428:440	arg1	DLPs					484:487	DLPs	484:487	DLPs	484:487	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	4	89	theme	acids	816:820	arg1	ratios					759:764	molar ratios	753:764	molar ratios of constituent monosaccharide	753:794	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	89	theme	acids	816:820	arg1	contents					827:834	contents	827:834	contents	827:834	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	89	theme	acids	816:820	arg1	viscosities					740:750	apparent viscosities	731:750	apparent viscosities	731:750	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	89	theme	acids	816:820	arg1	weights					722:728	The molecular weights	708:728	The molecular weights	708:728	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	4	89	theme	acids	816:820	arg1	contents					797:804	contents	797:804	contents of uronic acids	797:820	The molecular weights, apparent viscosities, molar ratios of constituent monosaccharide, contents of uronic acids, and contents of bonded polyphenolics in DLPs obtained by different drying techniques had noticeable variations, while the types of constituent monosaccharides and the major glycosidic linkages in DLPs were similar.					
33188812	1	90	theme	drying	239:244	arg1	drying					382:387	hot air drying	374:387	hot air drying	374:387	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	90	theme	drying	239:244	arg1	drying					274:279	radio frequency drying	258:279	radio frequency drying	258:279	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	90	theme	drying	239:244	arg1	drying					362:367	freezing drying	353:367	freezing drying	353:367	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	90	theme	drying	239:244	arg1	drying					295:300	microwave drying	285:300	microwave drying	285:300	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	90	theme	drying	239:244	arg1	techniques					246:255	innovative drying techniques	228:255	innovative drying techniques (radio frequency drying and microwave drying)	228:301	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
33188812	1	90	theme	drying	239:244	arg1	drying					345:350	vacuum drying	338:350	vacuum drying	338:350	The aim of this study was to well understand the impacts of innovative drying techniques (radio frequency drying and microwave drying) and traditional drying techniques (vacuum drying, freezing drying, and hot air drying) on the structural characteristics and bioactivities of polysaccharides from dandelion leaves (DLPs).					
32885971	0	0	theme	Dynamics	62:69	arg1	Simulations					71:81	Simple Atomistic Molecular Dynamics Simulations	35:81	Simple Atomistic Molecular Dynamics Simulations	35:81	The Answer Lies in the Energy: How Simple Atomistic Molecular Dynamics Simulations May Hold the Key to Epitope Prediction on the Fully Glycosylated SARS-CoV-2 Spike Protein.					
32885971	0	1	theme	Spike	159:163	arg1	Protein					165:171	the Fully Glycosylated SARS-CoV-2 Spike Protein	125:171	the Fully Glycosylated SARS-CoV-2 Spike Protein	125:171	The Answer Lies in the Energy: How Simple Atomistic Molecular Dynamics Simulations May Hold the Key to Epitope Prediction on the Fully Glycosylated SARS-CoV-2 Spike Protein.					
32885971	3	2	theme	original	447:454	arg1	approach					470:477	an original decomposition approach	444:477	an original decomposition approach	444:477	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	6	3	theme	peptidic	1135:1142	arg1	epitopes					1144:1151	underlying peptidic epitopes	1124:1151	underlying peptidic epitopes	1124:1151	Moreover, we detect two distinct behaviors for glycans: those with stronger energetic coupling are structurally relevant and protect underlying peptidic epitopes, and those with weaker coupling could themselves be prone to antibody recognition.					
32885971	0	4	theme	Molecular	52:60	arg1	Simulations					71:81	Simple Atomistic Molecular Dynamics Simulations	35:81	Simple Atomistic Molecular Dynamics Simulations	35:81	The Answer Lies in the Energy: How Simple Atomistic Molecular Dynamics Simulations May Hold the Key to Epitope Prediction on the Fully Glycosylated SARS-CoV-2 Spike Protein.					
32885971	0	5	theme	SARS-CoV-2	148:157	arg1	Protein					165:171	the Fully Glycosylated SARS-CoV-2 Spike Protein	125:171	the Fully Glycosylated SARS-CoV-2 Spike Protein	125:171	The Answer Lies in the Energy: How Simple Atomistic Molecular Dynamics Simulations May Hold the Key to Epitope Prediction on the Fully Glycosylated SARS-CoV-2 Spike Protein.					
32885971	3	6	theme	decomposition	456:468	arg1	approach					470:477	an original decomposition approach	444:477	an original decomposition approach	444:477	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	4	7	with	complex	803:809	arg1	nanobodies					825:834	nanobodies	825:834	nanobodies	825:834	Our results are validated by experimentally confirmed structures of S in complex with anti- or nanobodies.					
32885971	4	7	with	complex	803:809	arg1	anti-					816:820	anti-	816:820	anti-	816:820	Our results are validated by experimentally confirmed structures of S in complex with anti- or nanobodies.					
32885971	5	8	theme	host	894:897	arg1	epitopes					909:916	host (several) epitopes	894:916	host (several) epitopes	894:916	We identify poorly coupled subdomains that are poised to host (several) epitopes and potentially involved in large functional conformational transitions.					
32885971	3	9	theme	prior	648:652	arg1	knowledge					654:662	no prior knowledge	645:662	no prior knowledge of binding properties or ad hoc parameter combinations	645:717	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	2	10	theme	infection	271:279	arg1	mediator					259:266	the crucial mediator	247:266	the crucial mediator of infection	247:279	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	2	10	theme	infection	271:279	arg1	attention					346:354	the most attention	337:354	the most attention	337:354	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	5	11	theme	large	946:950	arg1	transitions					978:988	large functional conformational transitions	946:988	large functional conformational transitions	946:988	We identify poorly coupled subdomains that are poised to host (several) epitopes and potentially involved in large functional conformational transitions.					
32885971	2	12	theme	glycosylated	292:303	arg1	S					320:320	S	320:320	S	320:320	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	2	12	theme	glycosylated	292:303	arg1	protein					311:317	the viral glycosylated spike protein	282:317	the viral glycosylated spike protein (S)	282:321	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	5	13	theme	coupled	856:862	arg1	subdomains					864:873	poorly coupled subdomains	849:873	poorly coupled subdomains that are poised to host (several) epitopes and potentially involved in large functional conformational transitions	849:988	We identify poorly coupled subdomains that are poised to host (several) epitopes and potentially involved in large functional conformational transitions.					
32885971	3	14	theme	ad	689:690	arg1	combinations					706:717	ad hoc parameter combinations	689:717	ad hoc parameter combinations	689:717	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	15	theme	binding	667:673	arg1	properties					675:684	binding properties	667:684	binding properties	667:684	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	2	16	theme	crucial	251:257	arg1	mediator					259:266	the crucial mediator	247:266	the crucial mediator of infection	247:279	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	2	16	theme	crucial	251:257	arg1	attention					346:354	the most attention	337:354	the most attention	337:354	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	3	17	theme	properties	675:684	arg1	knowledge					654:662	no prior knowledge	645:662	no prior knowledge of binding properties or ad hoc parameter combinations	645:717	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	18	theme	binding	541:547	arg1	substructures					515:527	energetically uncoupled substructures	491:527	energetically uncoupled substructures	491:527	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	18	theme	binding	541:547	arg1	sites					549:553	antibody binding sites	532:553	antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed	532:727	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	19	theme	combinations	706:717	arg1	knowledge					654:662	no prior knowledge	645:662	no prior knowledge of binding properties or ad hoc parameter combinations	645:717	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	6	20	theme	antibody	1214:1221	arg1	recognition					1223:1233	antibody recognition	1214:1233	antibody recognition	1214:1233	Moreover, we detect two distinct behaviors for glycans: those with stronger energetic coupling are structurally relevant and protect underlying peptidic epitopes, and those with weaker coupling could themselves be prone to antibody recognition.					
32885971	2	21	theme	viral	286:290	arg1	S					320:320	S	320:320	S	320:320	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	2	21	theme	viral	286:290	arg1	protein					311:317	the viral glycosylated spike protein	282:317	the viral glycosylated spike protein (S)	282:321	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	2	22	theme	most	341:344	arg1	attention					346:354	the most attention	337:354	the most attention	337:354	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	2	22	theme	most	341:344	arg1	mediator					259:266	the crucial mediator	247:266	the crucial mediator of infection	247:279	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	6	23	theme	weaker	1169:1174	arg1	coupling					1176:1183	weaker coupling	1169:1183	weaker coupling	1169:1183	Moreover, we detect two distinct behaviors for glycans: those with stronger energetic coupling are structurally relevant and protect underlying peptidic epitopes, and those with weaker coupling could themselves be prone to antibody recognition.					
32885971	5	24	theme	several	900:906	arg1	epitopes					909:916	host (several) epitopes	894:916	host (several) epitopes	894:916	We identify poorly coupled subdomains that are poised to host (several) epitopes and potentially involved in large functional conformational transitions.					
32885971	3	25	dep	S.	581:582	arg1	simulations					632:642	unbiased MD simulations	620:642	unbiased MD simulations	620:642	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	25	dep	S.	581:582	arg1	all					595:597	all	595:597	all	595:597	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	2	26	theme	efforts	380:386	arg1	center					370:375	the center	366:375	the center of efforts	366:386	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	3	27	theme	hoc	692:694	arg1	combinations					706:717	ad hoc parameter combinations	689:717	ad hoc parameter combinations	689:717	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	28	theme	antibody	532:539	arg1	substructures					515:527	energetically uncoupled substructures	491:527	energetically uncoupled substructures	491:527	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	28	theme	antibody	532:539	arg1	sites					549:553	antibody binding sites	532:553	antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed	532:727	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	6	29	theme	underlying	1124:1133	arg1	epitopes					1144:1151	underlying peptidic epitopes	1124:1151	underlying peptidic epitopes	1124:1151	Moreover, we detect two distinct behaviors for glycans: those with stronger energetic coupling are structurally relevant and protect underlying peptidic epitopes, and those with weaker coupling could themselves be prone to antibody recognition.					
32885971	3	30	theme	parameter	696:704	arg1	combinations					706:717	ad hoc parameter combinations	689:717	ad hoc parameter combinations	689:717	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	0	31	theme	Epitope	103:109	arg1	Prediction					111:120	Epitope Prediction	103:120	Epitope Prediction	103:120	The Answer Lies in the Energy: How Simple Atomistic Molecular Dynamics Simulations May Hold the Key to Epitope Prediction on the Fully Glycosylated SARS-CoV-2 Spike Protein.					
32885971	4	32	theme	confirmed	774:782	arg1	structures					784:793	experimentally confirmed structures	759:793	experimentally confirmed structures of S in complex with anti- or nanobodies	759:834	Our results are validated by experimentally confirmed structures of S in complex with anti- or nanobodies.					
32885971	4	33	from	S	798:798	arg1	complex					803:809	complex	803:809	complex with anti- or nanobodies	803:834	Our results are validated by experimentally confirmed structures of S in complex with anti- or nanobodies.					
32885971	1	34	theme	health	190:195	arg1	threat					197:202	a health threat	188:202	a health threat with dire socioeconomical consequences	188:241	SARS-CoV-2 is a health threat with dire socioeconomical consequences.					
32885971	1	34	theme	health	190:195	arg1	SARS-CoV-2					174:183	SARS-CoV-2	174:183	SARS-CoV-2	174:183	SARS-CoV-2 is a health threat with dire socioeconomical consequences.					
32885971	0	35	theme	Simple	35:40	arg1	Simulations					71:81	Simple Atomistic Molecular Dynamics Simulations	35:81	Simple Atomistic Molecular Dynamics Simulations	35:81	The Answer Lies in the Energy: How Simple Atomistic Molecular Dynamics Simulations May Hold the Key to Epitope Prediction on the Fully Glycosylated SARS-CoV-2 Spike Protein.					
32885971	4	36	theme	S	798:798	arg1	structures					784:793	experimentally confirmed structures	759:793	experimentally confirmed structures of S in complex with anti- or nanobodies	759:834	Our results are validated by experimentally confirmed structures of S in complex with anti- or nanobodies.					
32885971	4	37	from	complex	803:809	arg1	structures					784:793	experimentally confirmed structures	759:793	experimentally confirmed structures of S in complex with anti- or nanobodies	759:834	Our results are validated by experimentally confirmed structures of S in complex with anti- or nanobodies.					
32885971	4	38	from	structures	784:793	arg1	complex					803:809	complex	803:809	complex with anti- or nanobodies	803:834	Our results are validated by experimentally confirmed structures of S in complex with anti- or nanobodies.					
32885971	0	39	theme	Glycosylated	135:146	arg1	Protein					165:171	the Fully Glycosylated SARS-CoV-2 Spike Protein	125:171	the Fully Glycosylated SARS-CoV-2 Spike Protein	125:171	The Answer Lies in the Energy: How Simple Atomistic Molecular Dynamics Simulations May Hold the Key to Epitope Prediction on the Fully Glycosylated SARS-CoV-2 Spike Protein.					
32885971	3	40	theme	unbiased	620:627	arg1	simulations					632:642	unbiased MD simulations	620:642	unbiased MD simulations	620:642	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	40	theme	unbiased	620:627	arg1	all					595:597	all	595:597	all	595:597	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	41	theme	uncoupled	505:513	arg1	substructures					515:527	energetically uncoupled substructures	491:527	energetically uncoupled substructures	491:527	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	41	theme	uncoupled	505:513	arg1	sites					549:553	antibody binding sites	532:553	antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed	532:727	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	2	42	theme	spike	305:309	arg1	S					320:320	S	320:320	S	320:320	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	2	42	theme	spike	305:309	arg1	protein					311:317	the viral glycosylated spike protein	282:317	the viral glycosylated spike protein (S)	282:321	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	0	43	theme	Atomistic	42:50	arg1	Simulations					71:81	Simple Atomistic Molecular Dynamics Simulations	35:81	Simple Atomistic Molecular Dynamics Simulations	35:81	The Answer Lies in the Energy: How Simple Atomistic Molecular Dynamics Simulations May Hold the Key to Epitope Prediction on the Fully Glycosylated SARS-CoV-2 Spike Protein.					
32885971	3	44	theme	MD	629:630	arg1	simulations					632:642	unbiased MD simulations	620:642	unbiased MD simulations	620:642	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	3	44	theme	MD	629:630	arg1	all					595:597	all	595:597	all	595:597	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	5	45	theme	functional	952:961	arg1	transitions					978:988	large functional conformational transitions	946:988	large functional conformational transitions	946:988	We identify poorly coupled subdomains that are poised to host (several) epitopes and potentially involved in large functional conformational transitions.					
32885971	6	46	theme	distinct	1015:1022	arg1	behaviors					1024:1032	two distinct behaviors	1011:1032	two distinct behaviors for glycans	1011:1044	Moreover, we detect two distinct behaviors for glycans: those with stronger energetic coupling are structurally relevant and protect underlying peptidic epitopes, and those with weaker coupling could themselves be prone to antibody recognition.					
32885971	6	47	theme	energetic	1067:1075	arg1	coupling					1077:1084	stronger energetic coupling	1058:1084	stronger energetic coupling	1058:1084	Moreover, we detect two distinct behaviors for glycans: those with stronger energetic coupling are structurally relevant and protect underlying peptidic epitopes, and those with weaker coupling could themselves be prone to antibody recognition.					
32885971	3	48	from	sites	549:553	arg1	S.					581:582	the fully glycosylated S.	558:582	the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed	558:727	Herein, we use an original decomposition approach to identify energetically uncoupled substructures as antibody binding sites on the fully glycosylated S. Crucially, all that is required are unbiased MD simulations; no prior knowledge of binding properties or ad hoc parameter combinations is needed.					
32885971	1	49	theme	dire	209:212	arg1	consequences					230:241	dire socioeconomical consequences	209:241	dire socioeconomical consequences	209:241	SARS-CoV-2 is a health threat with dire socioeconomical consequences.					
32885971	2	50	gly	glycosylated	292:303	arg1	S					320:320	S	320:320	S	320:320	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	2	50	gly	glycosylated	292:303	arg1	protein					311:317	the viral glycosylated spike protein	282:317	the viral glycosylated spike protein (S)	282:321	As the crucial mediator of infection, the viral glycosylated spike protein (S) has attracted the most attention and is at the center of efforts to develop therapeutics and diagnostics.					
32885971	5	51	theme	conformational	963:976	arg1	transitions					978:988	large functional conformational transitions	946:988	large functional conformational transitions	946:988	We identify poorly coupled subdomains that are poised to host (several) epitopes and potentially involved in large functional conformational transitions.					
32885971	1	52	with	threat	197:202	arg1	consequences					230:241	dire socioeconomical consequences	209:241	dire socioeconomical consequences	209:241	SARS-CoV-2 is a health threat with dire socioeconomical consequences.					
32885971	6	53	theme	stronger	1058:1065	arg1	coupling					1077:1084	stronger energetic coupling	1058:1084	stronger energetic coupling	1058:1084	Moreover, we detect two distinct behaviors for glycans: those with stronger energetic coupling are structurally relevant and protect underlying peptidic epitopes, and those with weaker coupling could themselves be prone to antibody recognition.					
32885971	1	54	theme	socioeconomical	214:228	arg1	consequences					230:241	dire socioeconomical consequences	209:241	dire socioeconomical consequences	209:241	SARS-CoV-2 is a health threat with dire socioeconomical consequences.					
32340396	9	0	theme	differential	1550:1561	arg1	recognition					1563:1573	the differential recognition	1546:1573	the differential recognition between healthy and cancer cells by Tepary bean lectins	1546:1629	This would explain the differential recognition between healthy and cancer cells by Tepary bean lectins.					
32340396	8	1	theme	carbohydrate	1442:1453	arg1	recognition					1455:1465	carbohydrate recognition	1442:1465	carbohydrate recognition	1442:1465	rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding.					
32340396	9	2	theme	Tepary	1611:1616	arg1	lectins					1623:1629	Tepary bean lectins	1611:1629	Tepary bean lectins	1611:1629	This would explain the differential recognition between healthy and cancer cells by Tepary bean lectins.					
32340396	0	3	with	Bean	31:34	arg1	Recognition					74:84	Specific Recognition	65:84	Specific Recognition	65:84	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	7	4	theme	α1-6	1188:1191	arg1	fucose					1198:1203	α1-6 core fucose	1188:1203	α1-6 core fucose	1188:1203	The presence of α1-6 core fucose, also tumor-associated, improved carbohydrate recognition.					
32340396	1	5	theme	lectin	247:252	arg1	recognition					323:333	its differential recognition	306:333	its differential recognition for cancer-type glycoconjugates	306:365	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	1	5	theme	lectin	247:252	arg1	production					207:216	the production	203:216	the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure,	203:300	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	0	6	theme	Specific	65:72	arg1	Recognition					74:84	Specific Recognition	65:84	Specific Recognition	65:84	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	1	7	theme	differential	310:321	arg1	recognition					323:333	its differential recognition	306:333	its differential recognition for cancer-type glycoconjugates	306:365	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	4	8	theme	N-linked	806:813	arg1	glycan					815:820	a ~2.5 kDa N-linked glycan	795:820	a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket	795:871	Monomers also presented a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket.					
32340396	6	9	theme	glycan	1042:1047	arg1	array					1049:1053	glycan array	1042:1053	glycan array	1042:1053	Screening for potential rTBL-1 targets by glycan array identified 14 positive binders, all of which correspond to β1-6 branched N-glycans' characteristics of cancer cells.					
32340396	3	10	dep	cations	642:648	arg1	cations					642:648	two divalent cations	629:648	two divalent cations (Ca2+ and Mn2+) attached to each subunit	629:689	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	10	dep	cations	642:648	arg1	Mn2+					660:663	Mn2+	660:663	Mn2+	660:663	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	10	dep	cations	642:648	arg1	Ca2+					651:654	Ca2+	651:654	Ca2+	651:654	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	0	11	theme	Cancer-Associated	90:106	arg1	Glycans					108:114	Cancer-Associated Glycans	90:114	Cancer-Associated Glycans	90:114	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	6	12	theme	cancer	1158:1163	arg1	cells					1165:1169	cancer cells	1158:1169	cancer cells	1158:1169	Screening for potential rTBL-1 targets by glycan array identified 14 positive binders, all of which correspond to β1-6 branched N-glycans' characteristics of cancer cells.					
32340396	5	13	theme	interfaces	970:979	arg1	stabilization					949:961	the stabilization	945:961	the stabilization of the interfaces between protomers	945:997	It does not participate in carbohydrate recognition but contributes to the stabilization of the interfaces between protomers.					
32340396	3	14	theme	protein	523:529	arg1	Characterization					499:514	Characterization	499:514	Characterization of the protein	499:529	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	4	15	theme	located	822:828	arg1	glycan					815:820	a ~2.5 kDa N-linked glycan	795:820	a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket	795:871	Monomers also presented a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket.					
32340396	5	16	theme	carbohydrate	901:912	arg1	recognition					914:924	carbohydrate recognition	901:924	carbohydrate recognition	901:924	It does not participate in carbohydrate recognition but contributes to the stabilization of the interfaces between protomers.					
32340396	7	17	theme	tumor-associated	1211:1226	arg1	presence					1176:1183	The presence	1172:1183	The presence	1172:1183	The presence of α1-6 core fucose, also tumor-associated, improved carbohydrate recognition.					
32340396	1	18	theme	cancer-type	339:349	arg1	glycoconjugates					351:365	cancer-type glycoconjugates	339:365	cancer-type glycoconjugates	339:365	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	6	19	theme	branched	1119:1126	arg1	characteristics					1139:1153	β1-6 branched N-glycans' characteristics	1114:1153	β1-6 branched N-glycans' characteristics of cancer cells	1114:1169	Screening for potential rTBL-1 targets by glycan array identified 14 positive binders, all of which correspond to β1-6 branched N-glycans' characteristics of cancer cells.					
32340396	8	20	theme	broad	1286:1290	arg1	spectrum					1292:1299	a broad spectrum	1284:1299	a broad spectrum of mono- and disaccharides	1284:1326	rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding.					
32340396	2	21	theme	affinity	474:481	arg1	chromatography					483:496	nickel affinity chromatography	467:496	nickel affinity chromatography	467:496	rTBL-1 was expressed in Pichia pastoris, yielding 316 mg per liter of culture, and was purified by nickel affinity chromatography.					
32340396	8	22	theme	larger	1499:1504	arg1	ligands					1506:1512	larger ligands	1499:1512	larger ligands for binding	1499:1524	rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding.					
32340396	8	23	theme	mono-	1304:1308	arg1	spectrum					1292:1299	a broad spectrum	1284:1299	a broad spectrum of mono- and disaccharides	1284:1326	rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding.					
32340396	9	24	theme	cancer	1595:1600	arg1	cells					1602:1606	healthy and cancer cells	1583:1606	cells	1602:1606	This would explain the differential recognition between healthy and cancer cells by Tepary bean lectins.					
32340396	3	25	theme	Å	757:757	arg1	resolution					759:768	1.9 Å resolution	753:768	1.9 Å resolution	753:768	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	8	26	theme	titration	1356:1364	arg1	calorimetry					1366:1376	isothermal titration calorimetry	1345:1376	isothermal titration calorimetry (ITC)	1345:1382	rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding.					
32340396	8	26	theme	titration	1356:1364	arg1	ITC					1379:1381	ITC	1379:1381	ITC	1379:1381	rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding.					
32340396	0	27	from	Bean	31:34	arg1	Lectin					12:17	Recombinant Lectin	0:17	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.	0:183	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	4	28	theme	kDa	802:804	arg1	glycan					815:820	a ~2.5 kDa N-linked glycan	795:820	a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket	795:871	Monomers also presented a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket.					
32340396	6	29	theme	rTBL-1	1024:1029	arg1	targets					1031:1037	potential rTBL-1 targets	1014:1037	potential rTBL-1 targets	1014:1037	Screening for potential rTBL-1 targets by glycan array identified 14 positive binders, all of which correspond to β1-6 branched N-glycans' characteristics of cancer cells.					
32340396	0	30	theme	Recombinant	0:10	arg1	Lectin					12:17	Recombinant Lectin	0:17	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.	0:183	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	4	31	theme	~2.5	797:800	arg1	kDa					802:804	kDa	802:804	kDa	802:804	Monomers also presented a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket.					
32340396	8	32	theme	disaccharides	1314:1326	arg1	spectrum					1292:1299	a broad spectrum	1284:1299	a broad spectrum of mono- and disaccharides	1284:1326	rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding.					
32340396	3	33	theme	X-ray	732:736	arg1	diffraction					738:748	X-ray diffraction	732:748	X-ray diffraction	732:748	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	6	34	theme	potential	1014:1022	arg1	targets					1031:1037	potential rTBL-1 targets	1014:1037	potential rTBL-1 targets	1014:1037	Screening for potential rTBL-1 targets by glycan array identified 14 positive binders, all of which correspond to β1-6 branched N-glycans' characteristics of cancer cells.					
32340396	0	35	theme	Tepary	24:29	arg1	Bean					31:34	Tepary Bean	24:34	Tepary Bean (Phaseolus acutifolius) with Specific Recognition	24:84	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	0	35	theme	Tepary	24:29	arg1	acutifolius					47:57	Phaseolus acutifolius	37:57	Phaseolus acutifolius	37:57	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	3	36	theme	3D	709:710	arg1	structure					712:720	its 3D structure	705:720	its 3D structure solved by X-ray diffraction at 1.9 Å resolution	705:768	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	4	37	theme	binding	858:864	arg1	pocket					866:871	the binding pocket	854:871	the binding pocket	854:871	Monomers also presented a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket.					
32340396	9	38	theme	healthy	1583:1589	arg1	cells					1602:1606	healthy and cancer cells	1583:1606	cells	1602:1606	This would explain the differential recognition between healthy and cancer cells by Tepary bean lectins.					
32340396	3	39	with	lectin	617:622	arg1	cations					642:648	two divalent cations	629:648	two divalent cations (Ca2+ and Mn2+) attached to each subunit	629:689	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	39	with	lectin	617:622	arg1	Mn2+					660:663	Mn2+	660:663	Mn2+	660:663	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	39	with	lectin	617:622	arg1	Ca2+					651:654	Ca2+	651:654	Ca2+	651:654	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	4	40	link	N-linked	806:813	arg1	glycan					815:820	a ~2.5 kDa N-linked glycan	795:820	a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket	795:871	Monomers also presented a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket.					
32340396	1	41	theme	three-dimensional	268:284	arg1	structure					291:299	its three-dimensional (3D) structure	264:299	its three-dimensional (3D) structure	264:299	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	1	41	theme	three-dimensional	268:284	arg1	lectin					247:252	a recombinant Tepary bean lectin	221:252	a recombinant Tepary bean lectin (rTBL-1)	221:261	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	4	42	theme	pocket	866:871	arg1	face					846:849	the opposite face	833:849	the opposite face of the binding pocket	833:871	Monomers also presented a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket.					
32340396	7	43	theme	core	1193:1196	arg1	fucose					1198:1203	α1-6 core fucose	1188:1203	α1-6 core fucose	1188:1203	The presence of α1-6 core fucose, also tumor-associated, improved carbohydrate recognition.					
32340396	3	44	theme	kDa	566:568	arg1	rTBL-1					543:548	rTBL-1	543:548	rTBL-1	543:548	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	44	theme	kDa	566:568	arg1	homo-tetramer					570:582	a stable 120 kDa homo-tetramer	553:582	a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution	553:768	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	7	45	theme	carbohydrate	1238:1249	arg1	recognition					1251:1261	carbohydrate recognition	1238:1261	carbohydrate recognition	1238:1261	The presence of α1-6 core fucose, also tumor-associated, improved carbohydrate recognition.					
32340396	0	46	theme	Structural	129:138	arg1	Characterization					140:155	Structural Characterization	129:155	Structural Characterization	129:155	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	3	47	theme	leguminous	606:615	arg1	lectin					617:622	a canonical leguminous lectin	594:622	a canonical leguminous lectin	594:622	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	8	48	theme	isothermal	1345:1354	arg1	calorimetry					1366:1376	isothermal titration calorimetry	1345:1376	isothermal titration calorimetry (ITC)	1345:1382	rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding.					
32340396	8	48	theme	isothermal	1345:1354	arg1	ITC					1379:1381	ITC	1379:1381	ITC	1379:1381	rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding.					
32340396	2	49	theme	Pichia	392:397	arg1	pastoris					399:406	Pichia pastoris	392:406	Pichia pastoris	392:406	rTBL-1 was expressed in Pichia pastoris, yielding 316 mg per liter of culture, and was purified by nickel affinity chromatography.					
32340396	2	50	theme	culture	438:444	arg1	liter					429:433	liter	429:433	liter of culture	429:444	rTBL-1 was expressed in Pichia pastoris, yielding 316 mg per liter of culture, and was purified by nickel affinity chromatography.					
32340396	3	51	attach	attached	666:673	arg2	Ca2+					651:654	Ca2+	651:654	Ca2+	651:654	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	51	attach	attached	666:673	arg2	cations					642:648	two divalent cations	629:648	two divalent cations (Ca2+ and Mn2+) attached to each subunit	629:689	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	51	attach	attached	666:673	arg1	subunit					683:689	each subunit	678:689	each subunit	678:689	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	51	attach	attached	666:673	arg2	Mn2+					660:663	Mn2+	660:663	Mn2+	660:663	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	4	52	theme	opposite	837:844	arg1	face					846:849	the opposite face	833:849	the opposite face of the binding pocket	833:871	Monomers also presented a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket.					
32340396	3	53	theme	stable	555:560	arg1	rTBL-1					543:548	rTBL-1	543:548	rTBL-1	543:548	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	53	theme	stable	555:560	arg1	homo-tetramer					570:582	a stable 120 kDa homo-tetramer	553:582	a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution	553:768	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	0	54	theme	Phaseolus	37:45	arg1	Bean					31:34	Tepary Bean	24:34	Tepary Bean (Phaseolus acutifolius) with Specific Recognition	24:84	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	0	54	theme	Phaseolus	37:45	arg1	acutifolius					47:57	Phaseolus acutifolius	37:57	Phaseolus acutifolius	37:57	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	3	55	theme	120	562:564	arg1	kDa					566:568	kDa	566:568	kDa	566:568	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	9	56	theme	bean	1618:1621	arg1	lectins					1623:1629	Tepary bean lectins	1611:1629	Tepary bean lectins	1611:1629	This would explain the differential recognition between healthy and cancer cells by Tepary bean lectins.					
32340396	6	57	theme	cells	1165:1169	arg1	characteristics					1139:1153	β1-6 branched N-glycans' characteristics	1114:1153	β1-6 branched N-glycans' characteristics of cancer cells	1114:1169	Screening for potential rTBL-1 targets by glycan array identified 14 positive binders, all of which correspond to β1-6 branched N-glycans' characteristics of cancer cells.					
32340396	8	58	theme	rTBL-1	1264:1269	arg1	affinity					1271:1278	rTBL-1 affinity	1264:1278	rTBL-1 affinity for a broad spectrum of mono- and disaccharides	1264:1326	rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding.					
32340396	0	59	theme	Target	162:167	arg1	Identification					169:182	Target Identification	162:182	Target Identification	162:182	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	3	60	theme	divalent	633:640	arg1	cations					642:648	two divalent cations	629:648	two divalent cations (Ca2+ and Mn2+) attached to each subunit	629:689	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	60	theme	divalent	633:640	arg1	Mn2+					660:663	Mn2+	660:663	Mn2+	660:663	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	60	theme	divalent	633:640	arg1	Ca2+					651:654	Ca2+	651:654	Ca2+	651:654	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	3	61	theme	canonical	596:604	arg1	lectin					617:622	a canonical leguminous lectin	594:622	a canonical leguminous lectin	594:622	Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution.					
32340396	6	62	theme	positive	1069:1076	arg1	binders					1078:1084	14 positive binders	1066:1084	14 positive binders	1066:1084	Screening for potential rTBL-1 targets by glycan array identified 14 positive binders, all of which correspond to β1-6 branched N-glycans' characteristics of cancer cells.					
32340396	1	63	theme	recombinant	223:233	arg1	rTBL-1					255:260	rTBL-1	255:260	rTBL-1	255:260	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	1	63	theme	recombinant	223:233	arg1	structure					291:299	its three-dimensional (3D) structure	264:299	its three-dimensional (3D) structure	264:299	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	1	63	theme	recombinant	223:233	arg1	lectin					247:252	a recombinant Tepary bean lectin	221:252	a recombinant Tepary bean lectin (rTBL-1)	221:261	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	2	64	theme	nickel	467:472	arg1	chromatography					483:496	nickel affinity chromatography	467:496	nickel affinity chromatography	467:496	rTBL-1 was expressed in Pichia pastoris, yielding 316 mg per liter of culture, and was purified by nickel affinity chromatography.					
32340396	0	65	dep	Lectin	12:17	arg1	Production					117:126	Production	117:126	Production	117:126	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	0	65	dep	Lectin	12:17	arg1	Characterization					140:155	Structural Characterization	129:155	Structural Characterization	129:155	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	0	65	dep	Lectin	12:17	arg1	Identification					169:182	Target Identification	162:182	Target Identification	162:182	Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification.					
32340396	6	66	theme	β1-6	1114:1117	arg1	characteristics					1139:1153	β1-6 branched N-glycans' characteristics	1114:1153	β1-6 branched N-glycans' characteristics of cancer cells	1114:1169	Screening for potential rTBL-1 targets by glycan array identified 14 positive binders, all of which correspond to β1-6 branched N-glycans' characteristics of cancer cells.					
32340396	7	67	theme	fucose	1198:1203	arg1	presence					1176:1183	The presence	1172:1183	The presence	1172:1183	The presence of α1-6 core fucose, also tumor-associated, improved carbohydrate recognition.					
32340396	1	68	theme	Tepary	235:240	arg1	rTBL-1					255:260	rTBL-1	255:260	rTBL-1	255:260	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	1	68	theme	Tepary	235:240	arg1	structure					291:299	its three-dimensional (3D) structure	264:299	its three-dimensional (3D) structure	264:299	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	1	68	theme	Tepary	235:240	arg1	lectin					247:252	a recombinant Tepary bean lectin	221:252	a recombinant Tepary bean lectin (rTBL-1)	221:261	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	1	69	dep	three-dimensional	268:284	arg1	3D					287:288	3D	287:288	3D	287:288	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	1	70	theme	bean	242:245	arg1	rTBL-1					255:260	rTBL-1	255:260	rTBL-1	255:260	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	1	70	theme	bean	242:245	arg1	structure					291:299	its three-dimensional (3D) structure	264:299	its three-dimensional (3D) structure	264:299	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
32340396	1	70	theme	bean	242:245	arg1	lectin					247:252	a recombinant Tepary bean lectin	221:252	a recombinant Tepary bean lectin (rTBL-1)	221:261	Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates.					
33556370	4	0	from	B. subtilis	686:696	arg1	present					675:681	present	675:681	present	675:681	However, bioinformatics predictions indicate that two other UTP-glucose-1-phosphate uridylyltransferases are present in B. subtilis.					
33556370	10	1	theme	other	1678:1682	arg1	components					1694:1703	potentially other cell wall components	1666:1703	potentially other cell wall components	1666:1703	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	6	2	theme	YngB	786:789	arg1	structure					773:781	The crystal structure	761:781	The crystal structure of YngB	761:789	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	2	3	theme	glucolipids	377:387	arg1	formation					364:372	the formation	360:372	the formation of glucolipids	360:387	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33556370	2	3	theme	glucolipids	377:387	arg1	decoration					295:304	the decoration	291:304	the decoration of wall teichoic acid (WTA) with glucose residues	291:354	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33556370	0	4	theme	glycolipid	75:84	arg1	formation					86:94	glycolipid formation	75:94	glycolipid formation	75:94	Bacillus subtilis YngB contributes to wall teichoic acid glucosylation and glycolipid formation during anaerobic growth.					
33556370	6	5	theme	functional	882:891	arg1	UGPase					893:898	a functional UGPase	880:898	a functional UGPase	880:898	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	9	6	theme	WT	1240:1241	arg1	strains					1266:1272	WT and mutant B. subtilis strains	1240:1272	WT and mutant B. subtilis strains	1240:1272	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	3	7	theme	morphological	543:555	arg1	defects					557:563	severe growth and morphological defects	525:563	defects	557:563	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	8	8	theme	gtaB	1072:1075	arg1	mutant					1077:1082	a B. subtilis gtaB mutant	1058:1082	a B. subtilis gtaB mutant	1058:1082	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	4	9	theme	UTP-glucose-1-phosphate	626:648	arg1	uridylyltransferases					650:669	two other UTP-glucose-1-phosphate uridylyltransferases	616:669	two other UTP-glucose-1-phosphate uridylyltransferases	616:669	However, bioinformatics predictions indicate that two other UTP-glucose-1-phosphate uridylyltransferases are present in B. subtilis.					
33556370	0	10	theme	anaerobic	103:111	arg1	growth					113:118	anaerobic growth	103:118	anaerobic growth	103:118	Bacillus subtilis YngB contributes to wall teichoic acid glucosylation and glycolipid formation during anaerobic growth.					
33556370	5	11	theme	one	737:739	arg1	function					725:732	the function	721:732	the function of one of them named YngB	721:758	Here, we investigated the function of one of them named YngB.					
33556370	8	12	theme	synthetic	1036:1044	arg1	promoter					1046:1053	a synthetic promoter	1034:1053	a synthetic promoter in a B. subtilis gtaB mutant	1034:1082	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	8	13	theme	UGPase	1219:1224	arg1	in vivo					1226:1232	UGPase in vivo	1219:1232	UGPase in vivo	1219:1232	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	6	14	theme	site	863:866	arg1	features					868:875	all necessary active site features	842:875	all necessary active site features of a functional UGPase	842:898	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	2	15	theme	acid	323:326	arg1	formation					364:372	the formation	360:372	the formation of glucolipids	360:387	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33556370	2	15	theme	acid	323:326	arg1	decoration					295:304	the decoration	291:304	the decoration of wall teichoic acid (WTA) with glucose residues	291:354	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33556370	7	16	theme	UGPase	914:919	arg1	activity					921:928	UGPase activity	914:928	UGPase activity	914:928	Furthermore, UGPase activity could be demonstrated in vitro using UTP and glucose-1-phosphate as substrates.					
33556370	10	17	theme	oxygen-limited	1753:1766	arg1	condition					1775:1783	oxygen-limited growth condition	1753:1783	oxygen-limited growth condition	1753:1783	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	3	18	theme	severe	525:530	arg1	growth					532:537	severe growth and morphological defects	525:563	growth	532:537	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	8	19	theme	residues	1126:1133	arg1	reintroduction					1100:1113	the reintroduction	1096:1113	the reintroduction of glucose residues on WTA and production of glycolipids	1096:1170	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	6	20	theme	active	856:861	arg1	features					868:875	all necessary active site features	842:875	all necessary active site features of a functional UGPase	842:898	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	2	21	theme	teichoic	314:321	arg1	acid					323:326	wall teichoic acid	309:326	wall teichoic acid (WTA) with glucose residues	309:354	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33556370	2	21	theme	teichoic	314:321	arg1	WTA					329:331	WTA	329:331	WTA	329:331	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33556370	3	22	theme	aerobic	473:479	arg1	conditions					488:497	standard aerobic growth conditions	464:497	standard aerobic growth conditions	464:497	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	10	23	theme	growth	1768:1773	arg1	condition					1775:1783	oxygen-limited growth condition	1753:1783	oxygen-limited growth condition	1753:1783	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	0	24	theme	wall	38:41	arg1	glucosylation					57:69	wall teichoic acid glucosylation	38:69	wall teichoic acid glucosylation	38:69	Bacillus subtilis YngB contributes to wall teichoic acid glucosylation and glycolipid formation during anaerobic growth.					
33556370	6	25	theme	necessary	846:854	arg1	features					868:875	all necessary active site features	842:875	all necessary active site features of a functional UGPase	842:898	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	2	26	theme	wall	309:312	arg1	acid					323:326	wall teichoic acid	309:326	wall teichoic acid (WTA) with glucose residues	309:354	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33556370	2	26	theme	wall	309:312	arg1	WTA					329:331	WTA	329:331	WTA	329:331	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33556370	6	27	theme	crystal	765:771	arg1	structure					773:781	The crystal structure	761:781	The crystal structure of YngB	761:789	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	3	28	theme	growth	481:486	arg1	conditions					488:497	standard aerobic growth conditions	464:497	standard aerobic growth conditions	464:497	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	9	29	from	production	1339:1348	arg1	WTA					1377:1379	WTA	1377:1379	WTA	1377:1379	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	8	30	theme	B. subtilis	1060:1070	arg1	mutant					1077:1082	a B. subtilis gtaB mutant	1058:1082	a B. subtilis gtaB mutant	1058:1082	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	2	31	theme	glucose	339:345	arg1	residues					347:354	glucose residues	339:354	glucose residues	339:354	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33556370	0	32	theme	Bacillus	0:7	arg1	YngB					18:21	Bacillus subtilis YngB	0:21	Bacillus subtilis YngB	0:21	Bacillus subtilis YngB contributes to wall teichoic acid glucosylation and glycolipid formation during anaerobic growth.					
33556370	9	33	theme	native	1442:1447	arg1	promoter					1449:1456	its native promoter	1438:1456	its native promoter	1438:1456	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	10	34	theme	operon	1542:1547	arg1	factor					1587:1592	the transcription factor	1569:1592	the transcription factor thought to be required for its expression	1569:1634	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	10	34	theme	operon	1542:1547	arg1	structure					1525:1533	the structure	1521:1533	the structure of the operon containing yngB	1521:1563	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	3	35	theme	standard	464:471	arg1	conditions					488:497	standard aerobic growth conditions	464:497	standard aerobic growth conditions	464:497	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	3	36	theme	B. subtilis	394:404	arg1	GtaB					413:416	The B. subtilis UGPase GtaB	390:416	The B. subtilis UGPase GtaB	390:416	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	3	36	theme	B. subtilis	394:404	arg1	essential					421:429	essential	421:429	essential	421:429	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	8	37	from	reintroduction	1100:1113	arg1	production					1146:1155	production	1146:1155	production	1146:1155	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	8	37	from	reintroduction	1100:1113	arg1	WTA					1138:1140	WTA	1138:1140	WTA	1138:1140	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	9	38	theme	B. subtilis	1254:1264	arg1	strains					1266:1272	WT and mutant B. subtilis strains	1240:1272	WT and mutant B. subtilis strains	1240:1272	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	10	39	contain	containing	1549:1558	arg2	yngB					1560:1563	yngB	1560:1563	yngB	1560:1563	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	10	39	contain	containing	1549:1558	arg1	operon					1542:1547	the operon	1538:1547	the operon containing yngB	1538:1563	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	3	40	theme	UGPase	406:411	arg1	GtaB					413:416	The B. subtilis UGPase GtaB	390:416	The B. subtilis UGPase GtaB	390:416	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	3	40	theme	UGPase	406:411	arg1	essential					421:429	essential	421:429	essential	421:429	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	4	41	theme	other	620:624	arg1	uridylyltransferases					650:669	two other UTP-glucose-1-phosphate uridylyltransferases	616:669	two other UTP-glucose-1-phosphate uridylyltransferases	616:669	However, bioinformatics predictions indicate that two other UTP-glucose-1-phosphate uridylyltransferases are present in B. subtilis.					
33556370	9	42	theme	anaerobic	1291:1299	arg1	conditions					1301:1310	anaerobic conditions	1291:1310	anaerobic conditions	1291:1310	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	9	43	theme	mutant	1247:1252	arg1	strains					1266:1272	WT and mutant B. subtilis strains	1240:1272	WT and mutant B. subtilis strains	1240:1272	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	3	44	theme	gtaB	504:507	arg1	mutants					509:515	gtaB mutants	504:515	gtaB mutants	504:515	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	3	45	theme	UDP-glucose	435:445	arg1	production					447:456	UDP-glucose production	435:456	UDP-glucose production under standard aerobic growth conditions	435:497	The B. subtilis UGPase GtaB is essential for UDP-glucose production under standard aerobic growth conditions, and gtaB mutants display severe growth and morphological defects.					
33556370	8	46	theme	glycolipids	1160:1170	arg1	production					1146:1155	production	1146:1155	production	1146:1155	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	8	46	theme	glycolipids	1160:1170	arg1	WTA					1138:1140	WTA	1138:1140	WTA	1138:1140	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	10	47	theme	glucose	1729:1735	arg1	residues					1737:1744	glucose residues	1729:1744	glucose residues	1729:1744	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	10	48	theme	cell	1684:1687	arg1	components					1694:1703	potentially other cell wall components	1666:1703	potentially other cell wall components	1666:1703	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	8	49	from	WTA	1138:1140	arg1	reintroduction					1100:1113	the reintroduction	1096:1113	the reintroduction of glucose residues on WTA and production of glycolipids	1096:1170	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	6	50	contain	has	817:819	arg2	fold					833:836	the typical fold	821:836	the typical fold	821:836	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	6	50	contain	has	817:819	arg2	features					868:875	all necessary active site features	842:875	all necessary active site features of a functional UGPase	842:898	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	6	50	contain	has	817:819	arg1	protein					809:815	the protein	805:815	the protein	805:815	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	6	51	theme	typical	825:831	arg1	fold					833:836	the typical fold	821:836	the typical fold	821:836	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	4	52	theme	bioinformatics	575:588	arg1	predictions					590:600	bioinformatics predictions	575:600	bioinformatics predictions	575:600	However, bioinformatics predictions indicate that two other UTP-glucose-1-phosphate uridylyltransferases are present in B. subtilis.					
33556370	0	53	theme	acid	52:55	arg1	glucosylation					57:69	wall teichoic acid glucosylation	38:69	wall teichoic acid glucosylation	38:69	Bacillus subtilis YngB contributes to wall teichoic acid glucosylation and glycolipid formation during anaerobic growth.					
33556370	4	54	from	present	675:681	arg1	B. subtilis					686:696	B. subtilis	686:696	B. subtilis	686:696	However, bioinformatics predictions indicate that two other UTP-glucose-1-phosphate uridylyltransferases are present in B. subtilis.					
33556370	5	55	theme	them	744:747	arg1	them					744:747	them	744:747	them	744:747	Here, we investigated the function of one of them named YngB.					
33556370	5	55	theme	them	744:747	arg1	one					737:739	one	737:739	one	737:739	Here, we investigated the function of one of them named YngB.					
33556370	9	56	theme	YngB-dependent	1313:1326	arg1	production					1339:1348	YngB-dependent glycolipid production	1313:1348	YngB-dependent glycolipid production	1313:1348	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	1	57	theme	UTP-glucose-1-phosphate	121:143	arg1	enzymes					170:176	enzymes	170:176	enzymes that produce UDP-glucose from UTP and glucose-1-phosphate	170:234	UTP-glucose-1-phosphate uridylyltransferases are enzymes that produce UDP-glucose from UTP and glucose-1-phosphate.					
33556370	1	57	theme	UTP-glucose-1-phosphate	121:143	arg1	uridylyltransferases					145:164	UTP-glucose-1-phosphate uridylyltransferases	121:164	UTP-glucose-1-phosphate uridylyltransferases	121:164	UTP-glucose-1-phosphate uridylyltransferases are enzymes that produce UDP-glucose from UTP and glucose-1-phosphate.					
33556370	0	58	theme	teichoic	43:50	arg1	glucosylation					57:69	wall teichoic acid glucosylation	38:69	wall teichoic acid glucosylation	38:69	Bacillus subtilis YngB contributes to wall teichoic acid glucosylation and glycolipid formation during anaerobic growth.					
33556370	8	59	from	residues	1126:1133	arg1	production					1146:1155	production	1146:1155	production	1146:1155	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	8	59	from	residues	1126:1133	arg1	WTA					1138:1140	WTA	1138:1140	WTA	1138:1140	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	9	60	theme	glycolipid	1328:1337	arg1	production					1339:1348	YngB-dependent glycolipid production	1313:1348	YngB-dependent glycolipid production	1313:1348	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	8	61	theme	glucose	1118:1124	arg1	residues					1126:1133	glucose residues	1118:1133	glucose residues on WTA and production of glycolipids	1118:1170	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	4	62	attach	present	675:681	arg1	B. subtilis					686:696	B. subtilis	686:696	B. subtilis	686:696	However, bioinformatics predictions indicate that two other UTP-glucose-1-phosphate uridylyltransferases are present in B. subtilis.					
33556370	4	62	attach	present	675:681	arg2	uridylyltransferases					650:669	two other UTP-glucose-1-phosphate uridylyltransferases	616:669	two other UTP-glucose-1-phosphate uridylyltransferases	616:669	However, bioinformatics predictions indicate that two other UTP-glucose-1-phosphate uridylyltransferases are present in B. subtilis.					
33556370	10	63	theme	wall	1689:1692	arg1	components					1694:1703	potentially other cell wall components	1666:1703	potentially other cell wall components	1666:1703	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	6	64	theme	UGPase	893:898	arg1	fold					833:836	the typical fold	821:836	the typical fold	821:836	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	6	64	theme	UGPase	893:898	arg1	features					868:875	all necessary active site features	842:875	all necessary active site features of a functional UGPase	842:898	The crystal structure of YngB revealed that the protein has the typical fold and all necessary active site features of a functional UGPase.					
33556370	8	65	from	promoter	1046:1053	arg1	Expression					1010:1019	Expression	1010:1019	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant	1010:1082	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	8	65	from	promoter	1046:1053	arg1	mutant					1077:1082	a B. subtilis gtaB mutant	1058:1082	a B. subtilis gtaB mutant	1058:1082	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	10	66	theme	transcription	1573:1585	arg1	factor					1587:1592	the transcription factor	1569:1592	the transcription factor thought to be required for its expression	1569:1634	Based on these findings, along with the structure of the operon containing yngB and the transcription factor thought to be required for its expression, we propose that besides WTA, potentially other cell wall components might be decorated with glucose residues during oxygen-limited growth condition.					
33556370	2	67	theme	Bacillus	240:247	arg1	subtilis					249:256	Bacillus subtilis 168	240:260	Bacillus subtilis 168	240:260	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33556370	8	68	theme	YngB	1024:1027	arg1	Expression					1010:1019	Expression	1010:1019	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant	1010:1082	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	4	69	located	present	675:681	arg1	B. subtilis					686:696	B. subtilis	686:696	B. subtilis	686:696	However, bioinformatics predictions indicate that two other UTP-glucose-1-phosphate uridylyltransferases are present in B. subtilis.					
33556370	4	69	located	present	675:681	arg2	uridylyltransferases					650:669	two other UTP-glucose-1-phosphate uridylyltransferases	616:669	two other UTP-glucose-1-phosphate uridylyltransferases	616:669	However, bioinformatics predictions indicate that two other UTP-glucose-1-phosphate uridylyltransferases are present in B. subtilis.					
33556370	9	70	theme	anaerobic	1464:1472	arg1	condition					1474:1482	anaerobic condition	1464:1482	anaerobic condition	1464:1482	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	9	71	theme	glucose	1354:1360	arg1	decorations					1362:1372	glucose decorations	1354:1372	glucose decorations on WTA	1354:1379	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	8	72	from	production	1146:1155	arg1	reintroduction					1100:1113	the reintroduction	1096:1113	the reintroduction of glucose residues on WTA and production of glycolipids	1096:1170	Expression of YngB from a synthetic promoter in a B. subtilis gtaB mutant resulted in the reintroduction of glucose residues on WTA and production of glycolipids, demonstrating that the enzyme can function as UGPase in vivo.					
33556370	9	73	from	decorations	1362:1372	arg1	WTA					1377:1379	WTA	1377:1379	WTA	1377:1379	When WT and mutant B. subtilis strains were grown under anaerobic conditions, YngB-dependent glycolipid production and glucose decorations on WTA could be detected, revealing that YngB is expressed from its native promoter under anaerobic condition.					
33556370	0	74	dep	Bacillus	0:7	arg1	subtilis					9:16	subtilis	9:16	subtilis	9:16	Bacillus subtilis YngB contributes to wall teichoic acid glucosylation and glycolipid formation during anaerobic growth.					
33556370	2	75	with	acid	323:326	arg1	residues					347:354	glucose residues	339:354	glucose residues	339:354	In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids.					
33128395	2	0	theme	8-aminopyrene-1,3,6-trisulfonic	484:514	arg1	acid					516:519	8-aminopyrene-1,3,6-trisulfonic acid	484:519	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	2	0	theme	8-aminopyrene-1,3,6-trisulfonic	484:514	arg1	APTS					522:525	APTS	522:525	APTS	522:525	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	3	1	theme	glucose	744:750	arg1	values					762:767	their glucose unit (GU) values	738:767	their glucose unit (GU) values in tomato xylem sap	738:787	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	4	2	theme	indicators	1040:1049	arg1	database					1018:1025	an open access database	1003:1025	an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org)	1003:1185	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	2	3	theme	free	549:552	arg1	oligosaccharides					554:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	2	4	from	symptoms	451:458	arg1	oligosaccharides					554:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	4	5	theme	limited	884:890	arg1	number					892:897	the limited number	880:897	the limited number	880:897	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	1	6	theme	limited	252:258	arg1	number					260:265	a limited number	250:265	a limited number of studies that examine biological functions of N-glycan structures in plants	250:343	Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown.					
33128395	0	7	theme	xylem	97:101	arg1	sap					103:105	tomato xylem sap	90:105	tomato xylem sap for early detection of nutrient deficiency	90:148	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	0	8	from	Determination	0:12	arg1	sap					103:105	tomato xylem sap	90:105	tomato xylem sap for early detection of nutrient deficiency	90:148	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	1	9	from	functions	302:310	arg1	plants					338:343	plants	338:343	plants	338:343	Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown.					
33128395	1	10	from	knowledge	160:168	arg1	biosynthesis					180:191	glycan biosynthesis	173:191	glycan biosynthesis	173:191	Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown.					
33128395	1	10	from	knowledge	160:168	arg1	processing					197:206	processing	197:206	processing	197:206	Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown.					
33128395	3	11	theme	fluorescence	841:852	arg1	detection					854:862	laser induced fluorescence detection	827:862	laser induced fluorescence detection (CE-LIF)	827:871	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	3	11	theme	fluorescence	841:852	arg1	CE-LIF					865:870	CE-LIF	865:870	CE-LIF	865:870	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	0	12	theme	tomato	90:95	arg1	sap					103:105	tomato xylem sap	90:105	tomato xylem sap for early detection of nutrient deficiency	90:148	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	3	13	theme	GU	758:759	arg1	values					762:767	their glucose unit (GU) values	738:767	their glucose unit (GU) values in tomato xylem sap	738:787	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	4	14	dep	http	1163:1166	arg1	//glycoplants.org					1168:1184	//glycoplants.org	1168:1184	http://glycoplants.org	1163:1184	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	4	15	from	stress	1145:1150	arg1	plants					1155:1160	plants	1155:1160	plants (http://glycoplants.org)	1155:1185	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	3	16	from	values	762:767	arg1	sap					785:787	tomato xylem sap	772:787	tomato xylem sap	772:787	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	4	17	theme	nutrient	1118:1125	arg1	deficiency					1127:1136	the nutrient deficiency	1114:1136	the nutrient deficiency caused stress in plants (http://glycoplants.org)	1114:1185	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	2	18	theme	statistical	388:398	arg1	correlation					400:410	the statistical correlation	384:410	the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	384:569	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	2	19	from	changes	473:479	arg1	oligosaccharides					554:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	4	20	theme	access	1011:1016	arg1	database					1018:1025	an open access database	1003:1025	an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org)	1003:1185	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	4	21	dep	plants	1155:1160	arg1	http					1163:1166	http	1163:1166	http://glycoplants.org	1163:1184	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	1	22	theme	glycan	173:178	arg1	biosynthesis					180:191	glycan biosynthesis	173:191	glycan biosynthesis	173:191	Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown.					
33128395	3	23	theme	tomato	772:777	arg1	sap					785:787	tomato xylem sap	772:787	tomato xylem sap	772:787	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	2	24	theme	deficiency	440:449	arg1	symptoms					451:458	nutrient (nitrogen) deficiency symptoms	420:458	nutrient (nitrogen) deficiency symptoms of crops	420:467	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	1	25	theme	studies	270:276	arg1	number					260:265	a limited number	250:265	a limited number of studies that examine biological functions of N-glycan structures in plants	250:343	Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown.					
33128395	4	26	theme	open	1006:1009	arg1	database					1018:1025	an open access database	1003:1025	an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org)	1003:1185	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	3	27	theme	deficiency	590:599	arg1	symptoms					601:608	deficiency symptoms	590:608	deficiency symptoms	590:608	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	3	28	theme	capillary	796:804	arg1	electrophoresis					806:820	capillary electrophoresis	796:820	capillary electrophoresis with laser induced fluorescence detection (CE-LIF)	796:871	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	3	29	theme	APTS-labeled	672:683	arg1	oligosaccharides					703:718	APTS-labeled complex type free oligosaccharides	672:718	APTS-labeled complex type free oligosaccharides	672:718	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	0	30	theme	type	25:28	arg1	Determination					0:12	Determination	0:12	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.	0:149	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	3	31	theme	xylem	779:783	arg1	sap					785:787	tomato xylem sap	772:787	tomato xylem sap	772:787	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	0	32	theme	complex	17:23	arg1	type					25:28	complex type free, non-conjugated oligosaccharide glucose unit values	17:85	complex type free, non-conjugated oligosaccharide glucose unit values	17:85	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	0	33	theme	early	111:115	arg1	detection					117:125	early detection	111:125	early detection of nutrient deficiency	111:148	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	1	34	dep	are	240:242	arg1	remain					352:357	remain	352:357	remain virtually unknown	352:375	Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown.					
33128395	2	35	theme	crops	463:467	arg1	symptoms					451:458	nutrient (nitrogen) deficiency symptoms	420:458	nutrient (nitrogen) deficiency symptoms of crops	420:467	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	2	35	theme	crops	463:467	arg1	changes					473:479	changes	473:479	changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	473:569	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	2	36	theme	nutrient	420:427	arg1	symptoms					451:458	nutrient (nitrogen) deficiency symptoms	420:458	nutrient (nitrogen) deficiency symptoms of crops	420:467	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	1	37	theme	biological	291:300	arg1	functions					302:310	biological functions	291:310	biological functions of N-glycan structures in plants	291:343	Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown.					
33128395	0	38	theme	nutrient	130:137	arg1	deficiency					139:148	nutrient deficiency	130:148	nutrient deficiency	130:148	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	3	39	theme	complex	685:691	arg1	oligosaccharides					703:718	APTS-labeled complex type free oligosaccharides	672:718	APTS-labeled complex type free oligosaccharides	672:718	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	0	40	theme	free	30:33	arg1	values					80:85	free, non-conjugated oligosaccharide glucose unit values	30:85	complex type free, non-conjugated oligosaccharide glucose unit values	17:85	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	2	41	theme	type	544:547	arg1	oligosaccharides					554:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	4	42	theme	promising	1030:1038	arg1	structures					1067:1076	glycan structures	1060:1076	glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org)	1060:1185	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	4	42	theme	promising	1030:1038	arg1	indicators					1040:1049	promising indicators	1030:1049	promising indicators	1030:1049	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	0	43	theme	oligosaccharide	51:65	arg1	values					80:85	free, non-conjugated oligosaccharide glucose unit values	30:85	complex type free, non-conjugated oligosaccharide glucose unit values	17:85	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	2	44	theme	complex	536:542	arg1	oligosaccharides					554:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	2	45	theme	acid	516:519	arg1	oligosaccharides					554:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	3	46	theme	unit	752:755	arg1	values					762:767	their glucose unit (GU) values	738:767	their glucose unit (GU) values in tomato xylem sap	738:787	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	1	47	theme	N-glycan	315:322	arg1	structures					324:333	N-glycan structures	315:333	N-glycan structures	315:333	Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown.					
33128395	0	48	theme	deficiency	139:148	arg1	detection					117:125	early detection	111:125	early detection of nutrient deficiency	111:148	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	0	49	dep	free	30:33	arg1	non-conjugated					36:49	non-conjugated	36:49	non-conjugated	36:49	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	4	50	theme	structures	902:911	arg1	number					892:897	the limited number	880:897	the limited number	880:897	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	1	51	theme	structures	324:333	arg1	functions					302:310	biological functions	291:310	biological functions of N-glycan structures in plants	291:343	Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown.					
33128395	0	52	dep	type	25:28	arg1	values					80:85	free, non-conjugated oligosaccharide glucose unit values	30:85	complex type free, non-conjugated oligosaccharide glucose unit values	17:85	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	4	53	theme	glycan	1060:1065	arg1	structures					1067:1076	glycan structures	1060:1076	glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org)	1060:1185	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	4	53	theme	glycan	1060:1065	arg1	indicators					1040:1049	promising indicators	1030:1049	promising indicators	1030:1049	Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants (http://glycoplants.org).					
33128395	3	54	theme	type	693:696	arg1	oligosaccharides					703:718	APTS-labeled complex type free oligosaccharides	672:718	APTS-labeled complex type free oligosaccharides	672:718	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	3	55	theme	Kjeldahl	652:659	arg1	digestion					661:669	Kjeldahl digestion	652:669	Kjeldahl digestion	652:669	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	3	56	with	electrophoresis	806:820	arg1	detection					854:862	laser induced fluorescence detection	827:862	laser induced fluorescence detection (CE-LIF)	827:871	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	3	56	with	electrophoresis	806:820	arg1	CE-LIF					865:870	CE-LIF	865:870	CE-LIF	865:870	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	3	57	theme	induced	833:839	arg1	detection					854:862	laser induced fluorescence detection	827:862	laser induced fluorescence detection (CE-LIF)	827:871	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	3	57	theme	induced	833:839	arg1	CE-LIF					865:870	CE-LIF	865:870	CE-LIF	865:870	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	0	58	theme	unit	75:78	arg1	values					80:85	free, non-conjugated oligosaccharide glucose unit values	30:85	complex type free, non-conjugated oligosaccharide glucose unit values	17:85	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	3	59	theme	free	698:701	arg1	oligosaccharides					703:718	APTS-labeled complex type free oligosaccharides	672:718	APTS-labeled complex type free oligosaccharides	672:718	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	3	60	theme	multispectral	627:639	arg1	images					641:646	multispectral images	627:646	multispectral images	627:646	While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF).					
33128395	0	61	theme	glucose	67:73	arg1	values					80:85	free, non-conjugated oligosaccharide glucose unit values	30:85	complex type free, non-conjugated oligosaccharide glucose unit values	17:85	Determination of complex type free, non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency.					
33128395	2	62	theme	nitrogen	430:437	arg1	symptoms					451:458	nutrient (nitrogen) deficiency symptoms	420:458	nutrient (nitrogen) deficiency symptoms of crops	420:467	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
33128395	2	63	theme	-labeled	527:534	arg1	oligosaccharides					554:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides	484:569	Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported.					
34288669	1	0	theme	complex	350:356	arg1	glycoproteins					358:370	highly complex glycoproteins	343:370	highly complex glycoproteins	343:370	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	4	1	theme	chromatography-mass	891:909	arg1	HPLC-MS					925:931	HPLC-MS	925:931	HPLC-MS	925:931	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	1	theme	chromatography-mass	891:909	arg1	spectrometry					911:922	high-performance liquid chromatography-mass spectrometry	867:922	high-performance liquid chromatography-mass spectrometry (HPLC-MS)	867:932	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	2	2	from	levels	450:455	arg1	interpretation					398:411	systematic interpretation	387:411	systematic interpretation of this data at different structural levels	387:455	Nevertheless, systematic interpretation of this data at different structural levels remains an analytical challenge.					
34288669	4	3	theme	hCG	719:721	arg1	glycoforms					693:702	glycoforms	693:702	glycoforms of recombinant hCG (drug product Ovitrelle)	693:746	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	4	gly	glycoforms	693:702	arg1	hCG					719:721	recombinant hCG	707:721	recombinant hCG (drug product Ovitrelle)	707:746	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	4	gly	glycoforms	693:702	arg1	Ovitrelle					737:745	drug product Ovitrelle	724:745	drug product Ovitrelle	724:745	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	5	theme	protein	950:956	arg1	subunits					958:965	protein subunits	950:965	protein subunits	950:965	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	1	6	theme	glycoproteins	358:370	arg1	data					335:338	structural data	324:338	structural data of highly complex glycoproteins	324:370	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	0	7	theme	Mass	181:184	arg1	Spectrometry					186:197	Mass Spectrometry	181:197	Mass Spectrometry	181:197	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	6	8	theme	annotation	1373:1382	arg1	tool					1384:1387	a computational annotation tool	1357:1387	a computational annotation tool	1357:1387	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	4	9	theme	recombinant	707:717	arg1	hCG					719:721	recombinant hCG	707:721	recombinant hCG (drug product Ovitrelle)	707:746	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	9	theme	recombinant	707:717	arg1	Ovitrelle					737:745	drug product Ovitrelle	724:745	drug product Ovitrelle	724:745	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	6	10	theme	released	1305:1312	arg1	glycans					1314:1320	released glycans	1305:1320	released glycans	1305:1320	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	5	11	theme	hCG	1159:1161	arg1	glycoforms					1163:1172	hCG glycoforms	1159:1172	hCG glycoforms	1159:1172	Starting from the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals, it was possible to explore the chemical space of hCG glycoforms and elucidate the complexity that hides behind just 50 signals.					
34288669	5	12	theme	chemical	1141:1148	arg1	space					1150:1154	the chemical space	1137:1154	the chemical space of hCG glycoforms	1137:1172	Starting from the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals, it was possible to explore the chemical space of hCG glycoforms and elucidate the complexity that hides behind just 50 signals.					
34288669	6	13	theme	data	1274:1277	arg1	integration					1259:1269	Systematic, stepwise integration	1238:1269	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool	1238:1387	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	7	14	theme	quality	1461:1467	arg1	sialylation					1488:1498	sialylation	1488:1498	sialylation	1488:1498	Additionally, critical quality attributes such as sialylation and core fucosylation were compared for two batches of Ovitrelle to assess the potential product variability.					
34288669	7	14	theme	quality	1461:1467	arg1	fucosylation					1509:1520	core fucosylation	1504:1520	core fucosylation	1504:1520	Additionally, critical quality attributes such as sialylation and core fucosylation were compared for two batches of Ovitrelle to assess the potential product variability.					
34288669	7	14	theme	quality	1461:1467	arg1	attributes					1469:1478	critical quality attributes	1452:1478	critical quality attributes such as sialylation and core fucosylation	1452:1520	Additionally, critical quality attributes such as sialylation and core fucosylation were compared for two batches of Ovitrelle to assess the potential product variability.					
34288669	7	15	theme	potential	1579:1587	arg1	variability					1597:1607	the potential product variability	1575:1607	the potential product variability	1575:1607	Additionally, critical quality attributes such as sialylation and core fucosylation were compared for two batches of Ovitrelle to assess the potential product variability.					
34288669	4	16	theme	established	760:770	arg1	glycan					801:806	released glycan	792:806	released glycan	792:806	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	16	theme	established	760:770	arg1	techniques					772:781	established techniques	760:781	established techniques	760:781	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	16	theme	established	760:770	arg1	analysis					825:832	glycopeptide analysis	812:832	glycopeptide analysis	812:832	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	17	theme	product	729:735	arg1	hCG					719:721	recombinant hCG	707:721	recombinant hCG (drug product Ovitrelle)	707:746	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	17	theme	product	729:735	arg1	Ovitrelle					737:745	drug product Ovitrelle	724:745	drug product Ovitrelle	724:745	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	0	18	theme	Different	108:116	arg1	Levels					129:134	Different Structural Levels	108:134	Different Structural Levels of Human Chorionic Gonadotropin	108:166	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	6	19	theme	glycopeptides	1323:1335	arg1	levels					1295:1300	the levels	1291:1300	the levels of released glycans, glycopeptides, and subunits	1291:1349	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	3	20	theme	chorionic	555:563	arg1	system					527:532	a model system	519:532	a model system in this study	519:546	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	3	20	theme	chorionic	555:563	arg1	hCG					579:581	hCG	579:581	hCG	579:581	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	3	20	theme	chorionic	555:563	arg1	gonadotropin					565:576	human chorionic gonadotropin	549:576	human chorionic gonadotropin (hCG)	549:582	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	6	21	gly	glycopeptides	1323:1335	arg2	glycopeptides					1323:1335	glycopeptides	1323:1335	glycopeptides	1323:1335	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	6	22	theme	underlying	1415:1424	arg1	glycoforms					1426:1435	1031 underlying glycoforms	1410:1435	1031 underlying glycoforms	1410:1435	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	4	23	theme	novel	840:844	arg1	approaches					846:855	novel approaches	840:855	novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS)	840:932	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	7	24	theme	critical	1452:1459	arg1	sialylation					1488:1498	sialylation	1488:1498	sialylation	1488:1498	Additionally, critical quality attributes such as sialylation and core fucosylation were compared for two batches of Ovitrelle to assess the potential product variability.					
34288669	7	24	theme	critical	1452:1459	arg1	fucosylation					1509:1520	core fucosylation	1504:1520	core fucosylation	1504:1520	Additionally, critical quality attributes such as sialylation and core fucosylation were compared for two batches of Ovitrelle to assess the potential product variability.					
34288669	7	24	theme	critical	1452:1459	arg1	attributes					1469:1478	critical quality attributes	1452:1478	critical quality attributes such as sialylation and core fucosylation	1452:1520	Additionally, critical quality attributes such as sialylation and core fucosylation were compared for two batches of Ovitrelle to assess the potential product variability.					
34288669	2	25	theme	data	421:424	arg1	interpretation					398:411	systematic interpretation	387:411	systematic interpretation of this data at different structural levels	387:455	Nevertheless, systematic interpretation of this data at different structural levels remains an analytical challenge.					
34288669	4	26	gly	glycopeptide	812:823	arg2	glycopeptide					812:823	glycopeptide analysis	812:832	glycopeptide analysis	812:832	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	3	27	theme	human	549:553	arg1	system					527:532	a model system	519:532	a model system in this study	519:546	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	3	27	theme	human	549:553	arg1	hCG					579:581	hCG	579:581	hCG	579:581	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	3	27	theme	human	549:553	arg1	gonadotropin					565:576	human chorionic gonadotropin	549:576	human chorionic gonadotropin (hCG)	549:582	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	4	28	theme	high-performance	867:882	arg1	HPLC-MS					925:931	HPLC-MS	925:931	HPLC-MS	925:931	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	28	theme	high-performance	867:882	arg1	spectrometry					911:922	high-performance liquid chromatography-mass spectrometry	867:922	high-performance liquid chromatography-mass spectrometry (HPLC-MS)	867:932	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	29	theme	hCG	1008:1010	arg1	complex					1012:1018	the noncovalent hCG complex	992:1018	the noncovalent hCG complex	992:1018	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	1	30	gly	glycoproteins	358:370	arg1	glycoproteins					358:370	highly complex glycoproteins	343:370	highly complex glycoproteins	343:370	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	4	31	theme	native	971:976	arg1	MS					978:979	native MS	971:979	native MS	971:979	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	5	32	dep	behind	1214:1219	arg1	signals					1229:1235	just 50 signals	1221:1235	just 50 signals	1221:1235	Starting from the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals, it was possible to explore the chemical space of hCG glycoforms and elucidate the complexity that hides behind just 50 signals.					
34288669	3	33	from	system	527:532	arg1	study					542:546	this study	537:546	this study	537:546	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	1	34	theme	Modern	200:205	arg1	approaches					218:227	Modern analytical approaches	200:227	Modern analytical approaches employing high-resolution mass spectrometry (MS)	200:276	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	2	35	theme	analytical	468:477	arg1	challenge					479:487	an analytical challenge	465:487	an analytical challenge	465:487	Nevertheless, systematic interpretation of this data at different structural levels remains an analytical challenge.					
34288669	6	36	dep	Systematic	1238:1247	arg1	stepwise					1250:1257	stepwise	1250:1257	stepwise	1250:1257	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	6	37	theme	computational	1359:1371	arg1	tool					1384:1387	a computational annotation tool	1357:1387	a computational annotation tool	1357:1387	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	6	38	theme	Systematic	1238:1247	arg1	integration					1259:1269	Systematic, stepwise integration	1238:1269	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool	1238:1387	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	4	39	theme	liquid	884:889	arg1	HPLC-MS					925:931	HPLC-MS	925:931	HPLC-MS	925:931	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	39	theme	liquid	884:889	arg1	spectrometry					911:922	high-performance liquid chromatography-mass spectrometry	867:922	high-performance liquid chromatography-mass spectrometry (HPLC-MS)	867:932	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	1	40	theme	analytical	207:216	arg1	approaches					218:227	Modern analytical approaches	200:227	Modern analytical approaches employing high-resolution mass spectrometry (MS)	200:276	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	0	41	theme	Chemical	14:21	arg1	Space					23:27	the Chemical Space	10:27	the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes	10:85	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	1	42	theme	vast	309:312	arg1	amount					314:319	a vast amount	307:319	a vast amount of structural data of highly complex glycoproteins	307:370	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	1	42	theme	vast	309:312	arg1	data					335:338	structural data	324:338	structural data of highly complex glycoproteins	324:370	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	0	43	theme	Structural	118:127	arg1	Levels					129:134	Different Structural Levels	108:134	Different Structural Levels of Human Chorionic Gonadotropin	108:166	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	0	44	theme	Protein	32:38	arg1	Glycosylation					40:52	Protein Glycosylation	32:52	Protein Glycosylation	32:52	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	1	45	theme	amount	314:319	arg1	generation					293:302	the generation	289:302	the generation of a vast amount of structural data of highly complex glycoproteins	289:370	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	0	46	theme	Human	139:143	arg1	Gonadotropin					155:166	Human Chorionic Gonadotropin	139:166	Human Chorionic Gonadotropin	139:166	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	5	47	theme	mass	1052:1055	arg1	spectrum					1057:1064	the deconvoluted mass spectrum	1035:1064	the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals	1035:1107	Starting from the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals, it was possible to explore the chemical space of hCG glycoforms and elucidate the complexity that hides behind just 50 signals.					
34288669	2	48	theme	structural	439:448	arg1	levels					450:455	different structural levels	429:455	different structural levels	429:455	Nevertheless, systematic interpretation of this data at different structural levels remains an analytical challenge.					
34288669	5	49	theme	glycoforms	1163:1172	arg1	space					1150:1154	the chemical space	1137:1154	the chemical space of hCG glycoforms	1137:1172	Starting from the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals, it was possible to explore the chemical space of hCG glycoforms and elucidate the complexity that hides behind just 50 signals.					
34288669	6	50	theme	subunits	1342:1349	arg1	levels					1295:1300	the levels	1291:1300	the levels of released glycans, glycopeptides, and subunits	1291:1349	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	4	51	theme	glycopeptide	812:823	arg1	analysis					825:832	glycopeptide analysis	812:832	glycopeptide analysis	812:832	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	1	52	theme	high-resolution	239:253	arg1	MS					274:275	MS	274:275	MS	274:275	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	1	52	theme	high-resolution	239:253	arg1	spectrometry					260:271	high-resolution mass spectrometry	239:271	high-resolution mass spectrometry (MS)	239:276	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	1	53	theme	structural	324:333	arg1	data					335:338	structural data	324:338	structural data of highly complex glycoproteins	324:370	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	0	54	theme	Gonadotropin	155:166	arg1	Levels					129:134	Different Structural Levels	108:134	Different Structural Levels of Human Chorionic Gonadotropin	108:166	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	4	55	theme	noncovalent	996:1006	arg1	complex					1012:1018	the noncovalent hCG complex	992:1018	the noncovalent hCG complex	992:1018	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	3	56	gly	glycoprotein	494:505	arg1	glycoprotein					494:505	The glycoprotein	490:505	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG),	490:583	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	4	57	theme	drug	724:727	arg1	hCG					719:721	recombinant hCG	707:721	recombinant hCG (drug product Ovitrelle)	707:746	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	4	57	theme	drug	724:727	arg1	Ovitrelle					737:745	drug product Ovitrelle	724:745	drug product Ovitrelle	724:745	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	0	58	theme	Glycosylation	40:52	arg1	Space					23:27	the Chemical Space	10:27	the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes	10:85	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	1	59	theme	data	335:338	arg1	amount					314:319	a vast amount	307:319	a vast amount of structural data of highly complex glycoproteins	307:370	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	1	59	theme	data	335:338	arg1	data					335:338	structural data	324:338	structural data of highly complex glycoproteins	324:370	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	0	60	theme	Chorionic	145:153	arg1	Gonadotropin					155:166	Human Chorionic Gonadotropin	139:166	Human Chorionic Gonadotropin	139:166	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	5	61	theme	hCG	1077:1079	arg1	spectrum					1057:1064	the deconvoluted mass spectrum	1035:1064	the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals	1035:1107	Starting from the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals, it was possible to explore the chemical space of hCG glycoforms and elucidate the complexity that hides behind just 50 signals.					
34288669	5	62	theme	dimeric	1069:1075	arg1	hCG					1077:1079	dimeric hCG	1069:1079	dimeric hCG comprising about 50 signals	1069:1107	Starting from the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals, it was possible to explore the chemical space of hCG glycoforms and elucidate the complexity that hides behind just 50 signals.					
34288669	7	63	theme	Ovitrelle	1555:1563	arg1	batches					1544:1550	two batches	1540:1550	two batches of Ovitrelle	1540:1563	Additionally, critical quality attributes such as sialylation and core fucosylation were compared for two batches of Ovitrelle to assess the potential product variability.					
34288669	5	64	theme	deconvoluted	1039:1050	arg1	spectrum					1057:1064	the deconvoluted mass spectrum	1035:1064	the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals	1035:1107	Starting from the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals, it was possible to explore the chemical space of hCG glycoforms and elucidate the complexity that hides behind just 50 signals.					
34288669	4	65	theme	released	792:799	arg1	glycan					801:806	released glycan	792:806	released glycan	792:806	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	3	66	theme	glycosylated	633:644	arg1	subunits					646:653	two heavily glycosylated subunits	621:653	two heavily glycosylated subunits	621:653	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	4	67	theme	glycoforms	693:702	arg1	multitude					680:688	the multitude	676:688	the multitude of glycoforms of recombinant hCG (drug product Ovitrelle)	676:746	In order to unravel the multitude of glycoforms of recombinant hCG (drug product Ovitrelle), we combine established techniques, such as released glycan and glycopeptide analysis, with novel approaches employing high-performance liquid chromatography-mass spectrometry (HPLC-MS) to characterize protein subunits and native MS to analyze the noncovalent hCG complex.					
34288669	1	68	theme	mass	255:258	arg1	MS					274:275	MS	274:275	MS	274:275	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	1	68	theme	mass	255:258	arg1	spectrometry					260:271	high-resolution mass spectrometry	239:271	high-resolution mass spectrometry (MS)	239:276	Modern analytical approaches employing high-resolution mass spectrometry (MS) facilitate the generation of a vast amount of structural data of highly complex glycoproteins.					
34288669	6	69	theme	glycans	1314:1320	arg1	levels					1295:1300	the levels	1291:1300	the levels of released glycans, glycopeptides, and subunits	1291:1349	Systematic, stepwise integration of data obtained at the levels of released glycans, glycopeptides, and subunits using a computational annotation tool allowed us to reveal 1031 underlying glycoforms.					
34288669	3	70	theme	model	521:525	arg1	system					527:532	a model system	519:532	a model system in this study	519:546	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	3	70	theme	model	521:525	arg1	gonadotropin					565:576	human chorionic gonadotropin	549:576	human chorionic gonadotropin (hCG)	549:582	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	0	71	theme	Protein	69:75	arg1	Complexes					77:85	Noncovalent Protein Complexes	57:85	Noncovalent Protein Complexes	57:85	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	2	72	theme	different	429:437	arg1	levels					450:455	different structural levels	429:455	different structural levels	429:455	Nevertheless, systematic interpretation of this data at different structural levels remains an analytical challenge.					
34288669	7	73	theme	product	1589:1595	arg1	variability					1597:1607	the potential product variability	1575:1607	the potential product variability	1575:1607	Additionally, critical quality attributes such as sialylation and core fucosylation were compared for two batches of Ovitrelle to assess the potential product variability.					
34288669	5	74	gly	glycoforms	1163:1172	arg1	hCG					1159:1161	hCG glycoforms	1159:1172	hCG glycoforms	1159:1172	Starting from the deconvoluted mass spectrum of dimeric hCG comprising about 50 signals, it was possible to explore the chemical space of hCG glycoforms and elucidate the complexity that hides behind just 50 signals.					
34288669	3	75	gly	glycosylated	633:644	arg1	subunits					646:653	two heavily glycosylated subunits	621:653	two heavily glycosylated subunits	621:653	The glycoprotein utilized as a model system in this study, human chorionic gonadotropin (hCG), exists as a heterodimer composed of two heavily glycosylated subunits.					
34288669	7	76	theme	core	1504:1507	arg1	fucosylation					1509:1520	core fucosylation	1504:1520	core fucosylation	1504:1520	Additionally, critical quality attributes such as sialylation and core fucosylation were compared for two batches of Ovitrelle to assess the potential product variability.					
34288669	0	77	theme	Noncovalent	57:67	arg1	Complexes					77:85	Noncovalent Protein Complexes	57:85	Noncovalent Protein Complexes	57:85	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	0	78	from	Space	23:27	arg1	Complexes					77:85	Noncovalent Protein Complexes	57:85	Noncovalent Protein Complexes	57:85	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	0	79	dep	Exploring	0:8	arg1	Expedition					91:100	An Expedition	88:100	An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry	88:197	Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin by Employing Mass Spectrometry.					
34288669	2	80	theme	systematic	387:396	arg1	interpretation					398:411	systematic interpretation	387:411	systematic interpretation of this data at different structural levels	387:455	Nevertheless, systematic interpretation of this data at different structural levels remains an analytical challenge.					
34770995	0	0	theme	Evolutionary	91:102	arg1	Approach					104:111	an Evolutionary Approach	88:111	an Evolutionary Approach	88:111	Modulating Glycoside Hydrolase Activity between Hydrolysis and Transfer Reactions Using an Evolutionary Approach.					
34770995	5	1	theme	reaction	856:863	arg1	specificity					865:875	reaction specificity	856:875	reaction specificity	856:875	This method is based on the conservation of coevolving residues in spatial contacts associated with reaction specificity.					
34770995	8	2	theme	dynamic	1248:1254	arg1	simulations					1256:1266	Molecular dynamic simulations	1238:1266	Molecular dynamic simulations of the variants	1238:1282	Molecular dynamic simulations of the variants indicated changes in flexibility that can account for the modified T/H ratio.					
34770995	3	3	from	applications	565:576	arg1	fields					617:622	industrial, chemical, or biomedical fields	581:622	industrial, chemical, or biomedical fields	581:622	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	7	4	from	TmGTase	1169:1175	arg1	variant					1156:1162	the M279N variant	1146:1162	the M279N variant from TmGTase	1146:1175	The K98P/D99A/H222Q variant from TmAmyA doubled the transglycosydation/hydrolysis (T/H) ratio while the M279N variant from TmGTase increased the hydrolysis/transglycosidation ratio five-fold.					
34770995	5	5	theme	residues	811:818	arg1	conservation					784:795	the conservation	780:795	the conservation of coevolving residues in spatial contacts associated with reaction specificity	780:875	This method is based on the conservation of coevolving residues in spatial contacts associated with reaction specificity.					
34770995	2	6	theme	various	304:310	arg1	degrees					312:318	various degrees	304:318	various degrees	304:318	Many of these enzymes also perform transglycosylation in various degrees, ranging from secondary to predominant reactions.					
34770995	6	7	theme	reaction	1024:1031	arg1	specificity					1033:1043	the reaction specificity	1020:1043	the reaction specificity	1020:1043	To evaluate the algorithm, mutants of α-amylase (TmAmyA) and glucanotransferase (TmGTase) from Thermotoga maritima were constructed to modify the reaction specificity.					
34770995	3	8	theme	biomedical	606:615	arg1	fields					617:622	industrial, chemical, or biomedical fields	581:622	industrial, chemical, or biomedical fields	581:622	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	4	9	theme	computational	646:658	arg1	approach					660:667	a computational approach	644:667	a computational approach for decoding the determinant structural composition defining the reaction specificity	644:753	This work proposes a computational approach for decoding the determinant structural composition defining the reaction specificity.					
34770995	2	10	gly	transglycosylation	282:299	arg1	degrees					312:318	various degrees	304:318	various degrees	304:318	Many of these enzymes also perform transglycosylation in various degrees, ranging from secondary to predominant reactions.					
34770995	6	11	theme	Thermotoga	973:982	arg1	maritima					984:991	Thermotoga maritima	973:991	Thermotoga maritima	973:991	To evaluate the algorithm, mutants of α-amylase (TmAmyA) and glucanotransferase (TmGTase) from Thermotoga maritima were constructed to modify the reaction specificity.					
34770995	7	12	theme	five-fold	1227:1235	arg1	ratio					1221:1225	the hydrolysis/transglycosidation ratio five-fold	1187:1235	the hydrolysis/transglycosidation ratio five-fold	1187:1235	The K98P/D99A/H222Q variant from TmAmyA doubled the transglycosydation/hydrolysis (T/H) ratio while the M279N variant from TmGTase increased the hydrolysis/transglycosidation ratio five-fold.					
34770995	4	13	theme	structural	698:707	arg1	composition					709:719	the determinant structural composition	682:719	the determinant structural composition defining the reaction specificity	682:753	This work proposes a computational approach for decoding the determinant structural composition defining the reaction specificity.					
34770995	3	14	theme	chemical	593:600	arg1	fields					617:622	industrial, chemical, or biomedical fields	581:622	industrial, chemical, or biomedical fields	581:622	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	3	15	theme	designing	479:487	arg1	enzymes					489:495	modifying and designing enzymes	465:495	enzymes	489:495	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	3	16	theme	structural	382:391	arg1	determinants					393:404	structural determinants	382:404	structural determinants associated with GH13 family reaction specificity	382:453	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	4	17	theme	reaction	734:741	arg1	specificity					743:753	the reaction specificity	730:753	the reaction specificity	730:753	This work proposes a computational approach for decoding the determinant structural composition defining the reaction specificity.					
34770995	0	18	theme	Glycoside	11:19	arg1	Activity					31:38	Glycoside Hydrolase Activity	11:38	Glycoside Hydrolase Activity between Hydrolysis and Transfer Reactions	11:80	Modulating Glycoside Hydrolase Activity between Hydrolysis and Transfer Reactions Using an Evolutionary Approach.					
34770995	7	19	theme	hydrolysis/transglycosidation	1191:1219	arg1	ratio					1221:1225	the hydrolysis/transglycosidation ratio five-fold	1187:1235	the hydrolysis/transglycosidation ratio five-fold	1187:1235	The K98P/D99A/H222Q variant from TmAmyA doubled the transglycosydation/hydrolysis (T/H) ratio while the M279N variant from TmGTase increased the hydrolysis/transglycosidation ratio five-fold.					
34770995	9	20	theme	essential	1365:1373	arg1	contribution					1375:1386	An essential contribution	1362:1386	An essential contribution of the presented computational approach	1362:1426	An essential contribution of the presented computational approach is its capacity to identify residues outside of the active center that affect the reaction specificity.					
34770995	9	20	theme	essential	1365:1373	arg1	capacity					1435:1442	its capacity to identify residues outside of the active center that affect the reaction specificity	1431:1529	its capacity to identify residues outside of the active center that affect the reaction specificity	1431:1529	An essential contribution of the presented computational approach is its capacity to identify residues outside of the active center that affect the reaction specificity.					
34770995	8	21	theme	Molecular	1238:1246	arg1	simulations					1256:1266	Molecular dynamic simulations	1238:1266	Molecular dynamic simulations of the variants	1238:1282	Molecular dynamic simulations of the variants indicated changes in flexibility that can account for the modified T/H ratio.					
34770995	3	22	theme	modifying	465:473	arg1	enzymes					489:495	modifying and designing enzymes	465:495	enzymes	489:495	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	3	23	theme	reaction	434:441	arg1	specificity					443:453	GH13 family reaction specificity	422:453	GH13 family reaction specificity	422:453	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	9	24	theme	active	1480:1485	arg1	center					1487:1492	the active center	1476:1492	the active center	1476:1492	An essential contribution of the presented computational approach is its capacity to identify residues outside of the active center that affect the reaction specificity.					
34770995	1	25	theme	polysaccharides	202:216	arg1	hydrolysis					188:197	the hydrolysis	184:197	the hydrolysis of polysaccharides such as glycogen and starch	184:244	The proteins within the CAZy glycoside hydrolase family GH13 catalyze the hydrolysis of polysaccharides such as glycogen and starch.					
34770995	8	26	theme	modified	1342:1349	arg1	ratio					1355:1359	the modified T/H ratio	1338:1359	the modified T/H ratio	1338:1359	Molecular dynamic simulations of the variants indicated changes in flexibility that can account for the modified T/H ratio.					
34770995	7	27	theme	K98P/D99A/H222Q	1050:1064	arg1	variant					1066:1072	The K98P/D99A/H222Q variant	1046:1072	The K98P/D99A/H222Q variant from TmAmyA	1046:1084	The K98P/D99A/H222Q variant from TmAmyA doubled the transglycosydation/hydrolysis (T/H) ratio while the M279N variant from TmGTase increased the hydrolysis/transglycosidation ratio five-fold.					
34770995	3	28	theme	GH13	422:425	arg1	specificity					443:453	GH13 family reaction specificity	422:453	GH13 family reaction specificity	422:453	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	8	29	from	changes	1294:1300	arg1	flexibility					1305:1315	flexibility	1305:1315	flexibility	1305:1315	Molecular dynamic simulations of the variants indicated changes in flexibility that can account for the modified T/H ratio.					
34770995	1	30	theme	hydrolase	153:161	arg1	family					163:168	the CAZy glycoside hydrolase family GH13	134:173	the CAZy glycoside hydrolase family GH13	134:173	The proteins within the CAZy glycoside hydrolase family GH13 catalyze the hydrolysis of polysaccharides such as glycogen and starch.					
34770995	7	31	from	TmAmyA	1079:1084	arg1	variant					1066:1072	The K98P/D99A/H222Q variant	1046:1072	The K98P/D99A/H222Q variant from TmAmyA	1046:1084	The K98P/D99A/H222Q variant from TmAmyA doubled the transglycosydation/hydrolysis (T/H) ratio while the M279N variant from TmGTase increased the hydrolysis/transglycosidation ratio five-fold.					
34770995	5	32	theme	coevolving	800:809	arg1	residues					811:818	coevolving residues	800:818	coevolving residues	800:818	This method is based on the conservation of coevolving residues in spatial contacts associated with reaction specificity.					
34770995	6	33	theme	glucanotransferase	939:956	arg1	mutants					905:911	mutants	905:911	mutants of α-amylase (TmAmyA) and glucanotransferase (TmGTase) from Thermotoga maritima	905:991	To evaluate the algorithm, mutants of α-amylase (TmAmyA) and glucanotransferase (TmGTase) from Thermotoga maritima were constructed to modify the reaction specificity.					
34770995	3	34	theme	increased	502:510	arg1	specificity					512:522	increased specificity	502:522	increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields	502:622	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	0	35	theme	Transfer	63:70	arg1	Reactions					72:80	Transfer Reactions	63:80	Transfer Reactions	63:80	Modulating Glycoside Hydrolase Activity between Hydrolysis and Transfer Reactions Using an Evolutionary Approach.					
34770995	9	36	theme	reaction	1510:1517	arg1	specificity					1519:1529	the reaction specificity	1506:1529	the reaction specificity	1506:1529	An essential contribution of the presented computational approach is its capacity to identify residues outside of the active center that affect the reaction specificity.					
34770995	7	37	theme	M279N	1150:1154	arg1	variant					1156:1162	the M279N variant	1146:1162	the M279N variant from TmGTase	1146:1175	The K98P/D99A/H222Q variant from TmAmyA doubled the transglycosydation/hydrolysis (T/H) ratio while the M279N variant from TmGTase increased the hydrolysis/transglycosidation ratio five-fold.					
34770995	5	38	from	conservation	784:795	arg1	contacts					831:838	spatial contacts	823:838	spatial contacts associated with reaction specificity	823:875	This method is based on the conservation of coevolving residues in spatial contacts associated with reaction specificity.					
34770995	3	39	theme	individual	532:541	arg1	reactions					543:551	individual reactions	532:551	individual reactions	532:551	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	9	40	theme	presented	1395:1403	arg1	approach					1419:1426	the presented computational approach	1391:1426	the presented computational approach	1391:1426	An essential contribution of the presented computational approach is its capacity to identify residues outside of the active center that affect the reaction specificity.					
34770995	8	41	theme	T/H	1351:1353	arg1	ratio					1355:1359	the modified T/H ratio	1338:1359	the modified T/H ratio	1338:1359	Molecular dynamic simulations of the variants indicated changes in flexibility that can account for the modified T/H ratio.					
34770995	3	42	theme	further	557:563	arg1	applications					565:576	further applications	557:576	further applications in industrial, chemical, or biomedical fields	557:622	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	9	43	theme	computational	1405:1417	arg1	approach					1419:1426	the presented computational approach	1391:1426	the presented computational approach	1391:1426	An essential contribution of the presented computational approach is its capacity to identify residues outside of the active center that affect the reaction specificity.					
34770995	6	44	from	maritima	984:991	arg1	mutants					905:911	mutants	905:911	mutants of α-amylase (TmAmyA) and glucanotransferase (TmGTase) from Thermotoga maritima	905:991	To evaluate the algorithm, mutants of α-amylase (TmAmyA) and glucanotransferase (TmGTase) from Thermotoga maritima were constructed to modify the reaction specificity.					
34770995	7	45	theme	T/H	1129:1131	arg1	ratio					1134:1138	the transglycosydation/hydrolysis (T/H) ratio	1094:1138	the transglycosydation/hydrolysis (T/H) ratio	1094:1138	The K98P/D99A/H222Q variant from TmAmyA doubled the transglycosydation/hydrolysis (T/H) ratio while the M279N variant from TmGTase increased the hydrolysis/transglycosidation ratio five-fold.					
34770995	1	46	theme	CAZy	138:141	arg1	family					163:168	the CAZy glycoside hydrolase family GH13	134:173	the CAZy glycoside hydrolase family GH13	134:173	The proteins within the CAZy glycoside hydrolase family GH13 catalyze the hydrolysis of polysaccharides such as glycogen and starch.					
34770995	5	47	theme	spatial	823:829	arg1	contacts					831:838	spatial contacts	823:838	spatial contacts associated with reaction specificity	823:875	This method is based on the conservation of coevolving residues in spatial contacts associated with reaction specificity.					
34770995	3	48	with	enzymes	489:495	arg1	specificity					512:522	increased specificity	502:522	increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields	502:622	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	9	49	theme	approach	1419:1426	arg1	contribution					1375:1386	An essential contribution	1362:1386	An essential contribution of the presented computational approach	1362:1426	An essential contribution of the presented computational approach is its capacity to identify residues outside of the active center that affect the reaction specificity.					
34770995	9	49	theme	approach	1419:1426	arg1	capacity					1435:1442	its capacity to identify residues outside of the active center that affect the reaction specificity	1431:1529	its capacity to identify residues outside of the active center that affect the reaction specificity	1431:1529	An essential contribution of the presented computational approach is its capacity to identify residues outside of the active center that affect the reaction specificity.					
34770995	1	50	theme	glycoside	143:151	arg1	family					163:168	the CAZy glycoside hydrolase family GH13	134:173	the CAZy glycoside hydrolase family GH13	134:173	The proteins within the CAZy glycoside hydrolase family GH13 catalyze the hydrolysis of polysaccharides such as glycogen and starch.					
34770995	2	51	theme	predominant	347:357	arg1	reactions					359:367	predominant reactions	347:367	predominant reactions	347:367	Many of these enzymes also perform transglycosylation in various degrees, ranging from secondary to predominant reactions.					
34770995	8	52	theme	variants	1275:1282	arg1	simulations					1256:1266	Molecular dynamic simulations	1238:1266	Molecular dynamic simulations of the variants	1238:1282	Molecular dynamic simulations of the variants indicated changes in flexibility that can account for the modified T/H ratio.					
34770995	6	53	theme	α-amylase	916:924	arg1	mutants					905:911	mutants	905:911	mutants of α-amylase (TmAmyA) and glucanotransferase (TmGTase) from Thermotoga maritima	905:991	To evaluate the algorithm, mutants of α-amylase (TmAmyA) and glucanotransferase (TmGTase) from Thermotoga maritima were constructed to modify the reaction specificity.					
34770995	2	54	theme	enzymes	261:267	arg1	Many					247:250	Many	247:250	Many	247:250	Many of these enzymes also perform transglycosylation in various degrees, ranging from secondary to predominant reactions.					
34770995	2	54	theme	enzymes	261:267	arg1	enzymes					261:267	these enzymes	255:267	these enzymes	255:267	Many of these enzymes also perform transglycosylation in various degrees, ranging from secondary to predominant reactions.					
34770995	7	55	theme	transglycosydation/hydrolysis	1098:1126	arg1	ratio					1134:1138	the transglycosydation/hydrolysis (T/H) ratio	1094:1138	the transglycosydation/hydrolysis (T/H) ratio	1094:1138	The K98P/D99A/H222Q variant from TmAmyA doubled the transglycosydation/hydrolysis (T/H) ratio while the M279N variant from TmGTase increased the hydrolysis/transglycosidation ratio five-fold.					
34770995	3	56	theme	industrial	581:590	arg1	fields					617:622	industrial, chemical, or biomedical fields	581:622	industrial, chemical, or biomedical fields	581:622	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34770995	4	57	theme	determinant	686:696	arg1	composition					709:719	the determinant structural composition	682:719	the determinant structural composition defining the reaction specificity	682:753	This work proposes a computational approach for decoding the determinant structural composition defining the reaction specificity.					
34770995	0	58	theme	Hydrolase	21:29	arg1	Activity					31:38	Glycoside Hydrolase Activity	11:38	Glycoside Hydrolase Activity between Hydrolysis and Transfer Reactions	11:80	Modulating Glycoside Hydrolase Activity between Hydrolysis and Transfer Reactions Using an Evolutionary Approach.					
34770995	3	59	theme	family	427:432	arg1	specificity					443:453	GH13 family reaction specificity	422:453	GH13 family reaction specificity	422:453	Identifying structural determinants associated with GH13 family reaction specificity is key to modifying and designing enzymes with increased specificity towards individual reactions for further applications in industrial, chemical, or biomedical fields.					
34260212	3	0	theme	glycosylating	732:744	arg1	power					723:727	power	723:727	power	723:727	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	3	0	theme	glycosylating	732:744	arg1	agents					746:751	glycosylating agents	732:751	glycosylating agents	732:751	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	3	1	theme	methods	556:562	arg1	lack					538:541	the lack	534:541	the lack of synthetic methods for rare 6dHepp architectures of importance	534:606	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	1	2	theme	resistant	274:282	arg1	pathogens					284:292	highly resistant pathogens	267:292	highly resistant pathogens	267:292	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	6	3	theme	C2	1338:1339	arg1	configuration					1341:1353	the C2 configuration	1334:1353	the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride	1334:1531	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	3	4	theme	importance	597:606	arg1	architectures					580:592	rare 6dHepp architectures	568:592	rare 6dHepp architectures of importance	568:606	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	6	5	theme	efficient	1228:1236	arg1	construction					1238:1249	highly efficient construction	1221:1249	highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside	1221:1316	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	3	6	contain	have	718:721	arg1	fluorides					703:711	uncommon d-/l-6dHepp fluorides	682:711	uncommon d-/l-6dHepp fluorides that have power as glycosylating agents	682:751	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	3	6	contain	have	718:721	arg2	power					723:727	power	723:727	power	723:727	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	3	6	contain	have	718:721	arg2	agents					746:751	glycosylating agents	732:751	glycosylating agents	732:751	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	2	7	theme	C.	332:333	arg1	polysaccharides					351:365	The C. jejuni capsular polysaccharides	328:365	The C. jejuni capsular polysaccharides	328:365	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	2	7	theme	C.	332:333	arg1	antigens					451:458	potential antigens	441:458	potential antigens	441:458	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	1	8	theme	diarrheal	214:222	arg1	diseases					224:231	human diarrheal diseases	208:231	human diarrheal diseases	208:231	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	0	9	theme	Capsular	138:145	arg1	Polysaccharide					147:160	Campylobacter jejuni Strain CG8486 Capsular Polysaccharide	103:160	Campylobacter jejuni Strain CG8486 Capsular Polysaccharide	103:160	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.					
34260212	5	10	theme	jejuni	1128:1133	arg1	strain					1135:1140	C. jejuni strain CG8486	1125:1147	C. jejuni strain CG8486	1125:1147	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	6	11	theme	-d-ido-heptopyranoside	1295:1316	arg1	construction					1238:1249	highly efficient construction	1221:1249	highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside	1221:1316	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	5	12	dep	-β-d-GlcpNAc-	1053:1065	arg1	units					1070:1074	1→ units	1067:1074	1→ units	1067:1074	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	3	13	theme	efficient	640:648	arg1	approach					650:657	a novel and efficient approach	628:657	a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents	628:751	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	6	14	theme	1,2-cis	1287:1293	arg1	-d-ido-heptopyranoside					1295:1316	the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside	1254:1316	the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside	1254:1316	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	5	15	theme	capsular	1098:1105	arg1	polysaccharide					1107:1120	the capsular polysaccharide	1094:1120	the capsular polysaccharide of C. jejuni strain CG8486	1094:1147	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	3	16	theme	novel	630:634	arg1	approach					650:657	a novel and efficient approach	628:657	a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents	628:751	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	6	17	theme	β-	1284:1285	arg1	-d-ido-heptopyranoside					1295:1316	the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside	1254:1316	the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside	1254:1316	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	5	18	theme	hexasaccharide	1003:1016	arg1	-β-d-GlcpNAc-					1053:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	5	19	theme	first	1176:1180	arg1	time					1182:1185	the first time	1172:1185	the first time	1172:1185	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	7	20	theme	Campylobacter	1700:1712	arg1	infection					1714:1722	Campylobacter infection	1700:1722	Campylobacter infection	1700:1722	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	6	21	theme	stereoselective	1454:1468	arg1	glycosylation					1470:1482	anchimerically assisted stereoselective glycosylation	1430:1482	anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride	1430:1531	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	1	22	theme	leading	191:197	arg1	cause					199:203	the leading cause	187:203	the leading cause of human diarrheal diseases	187:231	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	1	22	theme	leading	191:197	arg1	jejuni					177:182	Campylobacter jejuni	163:182	Campylobacter jejuni	163:182	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	7	23	theme	vaccines	1682:1689	arg1	development					1657:1667	the further development	1645:1667	the further development of synthetic vaccines fighting Campylobacter infection	1645:1722	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	0	24	theme	Campylobacter	103:115	arg1	jejuni					117:122	Campylobacter jejuni	103:122	Campylobacter jejuni Strain CG8486 Capsular Polysaccharide	103:160	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.					
34260212	1	25	theme	Campylobacter	163:175	arg1	one					260:262	one	260:262	one	260:262	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	1	25	theme	Campylobacter	163:175	arg1	cause					199:203	the leading cause	187:203	the leading cause of human diarrheal diseases	187:231	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	1	25	theme	Campylobacter	163:175	arg1	pathogens					284:292	highly resistant pathogens	267:292	highly resistant pathogens	267:292	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	1	25	theme	Campylobacter	163:175	arg1	jejuni					177:182	Campylobacter jejuni	163:182	Campylobacter jejuni	163:182	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	4	26	theme	decarboxylative	829:843	arg1	fluorination					845:856	radical decarboxylative fluorination	821:856	radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides	821:924	The synthesis is achieved by a C1-to-C5 switch strategy relying on radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides.					
34260212	0	27	theme	Strain	124:129	arg1	Polysaccharide					147:160	Campylobacter jejuni Strain CG8486 Capsular Polysaccharide	103:160	Campylobacter jejuni Strain CG8486 Capsular Polysaccharide	103:160	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.					
34260212	2	28	theme	antibacterial	482:494	arg1	vaccines					513:520	innovative antibacterial glycoconjugation vaccines	471:520	innovative antibacterial glycoconjugation vaccines	471:520	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	4	29	theme	C1-to-C5	785:792	arg1	strategy					801:808	a C1-to-C5 switch strategy	783:808	a C1-to-C5 switch strategy relying on radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides	783:924	The synthesis is achieved by a C1-to-C5 switch strategy relying on radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides.					
34260212	5	30	theme	of	1027:1028	arg1	-β-d-GlcpNAc-					1053:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	2	31	theme	uncommon	394:401	arg1	residues					410:417	uncommon 6dHepp residues	394:417	uncommon 6dHepp residues	394:417	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	0	32	theme	Rare	13:16	arg1	Fluorides					46:54	Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides	13:54	Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides	13:54	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.					
34260212	1	33	theme	Health	307:312	arg1	Organization					314:325	the World Health Organization	297:325	the World Health Organization	297:325	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	3	34	theme	fluorides	703:711	arg1	preparation					667:677	the preparation	663:677	the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents	663:751	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	0	35	theme	Fluorides	46:54	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.	0:161	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.					
34260212	6	36	theme	6dgulHepp	1514:1522	arg1	fluoride					1524:1531	the orthogonally protected 6dgulHepp fluoride	1487:1531	the orthogonally protected 6dgulHepp fluoride	1487:1531	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	3	37	theme	uncommon	682:689	arg1	fluorides					703:711	uncommon d-/l-6dHepp fluorides	682:711	uncommon d-/l-6dHepp fluorides that have power as glycosylating agents	682:751	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	4	38	theme	available	895:903	arg1	d-C-glycosides					911:924	readily available allyl d-C-glycosides	887:924	readily available allyl d-C-glycosides	887:924	The synthesis is achieved by a C1-to-C5 switch strategy relying on radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides.					
34260212	2	39	theme	broad	375:379	arg1	existence					381:389	broad existence	375:389	broad existence of uncommon 6dHepp residues	375:417	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	7	40	theme	rational	1617:1624	arg1	glycans					1592:1598	the resulting glycans	1578:1598	the resulting glycans	1578:1598	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	7	40	theme	rational	1617:1624	arg1	point					1635:1639	a rational starting point	1615:1639	a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection	1615:1722	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	3	41	gly	glycosylating	732:744	arg0	agents					746:751	glycosylating agents	732:751	glycosylating agents	732:751	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	3	41	gly	glycosylating	732:744	arg0	power					723:727	power	723:727	power	723:727	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	4	42	theme	acids	868:872	arg1	fluorination					845:856	radical decarboxylative fluorination	821:856	radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides	821:924	The synthesis is achieved by a C1-to-C5 switch strategy relying on radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides.					
34260212	2	43	theme	6dHepp	403:408	arg1	residues					410:417	uncommon 6dHepp residues	394:417	uncommon 6dHepp residues	394:417	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	6	44	gly	glycosylation	1470:1482	arg1	fluoride					1524:1531	the orthogonally protected 6dgulHepp fluoride	1487:1531	the orthogonally protected 6dgulHepp fluoride	1487:1531	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	6	45	theme	β-	1358:1359	arg1	-d-gulo-heptopyranoside					1371:1393	β-(1,2-trans)-d-gulo-heptopyranoside	1358:1393	β-(1,2-trans)-d-gulo-heptopyranoside	1358:1393	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	3	46	theme	synthetic	546:554	arg1	methods					556:562	synthetic methods	546:562	synthetic methods	546:562	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	7	47	theme	resulting	1582:1590	arg1	glycans					1592:1598	the resulting glycans	1578:1598	the resulting glycans	1578:1598	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	7	47	theme	resulting	1582:1590	arg1	point					1635:1639	a rational starting point	1615:1639	a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection	1615:1722	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	6	48	theme	configuration	1341:1353	arg1	inversion					1321:1329	inversion	1321:1329	inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride	1321:1531	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	1	49	theme	pathogens	284:292	arg1	one					260:262	one	260:262	one	260:262	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	1	49	theme	pathogens	284:292	arg1	pathogens					284:292	highly resistant pathogens	267:292	highly resistant pathogens	267:292	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	1	49	theme	pathogens	284:292	arg1	jejuni					177:182	Campylobacter jejuni	163:182	Campylobacter jejuni	163:182	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	3	50	theme	6dHepp	573:578	arg1	architectures					580:592	rare 6dHepp architectures	568:592	rare 6dHepp architectures of importance	568:606	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	7	51	theme	6dHepp	1557:1562	arg1	accessibility					1540:1552	Ready accessibility	1534:1552	Ready accessibility of 6dHepp	1534:1562	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	4	52	theme	allyl	905:909	arg1	d-C-glycosides					911:924	readily available allyl d-C-glycosides	887:924	readily available allyl d-C-glycosides	887:924	The synthesis is achieved by a C1-to-C5 switch strategy relying on radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides.					
34260212	7	53	theme	Ready	1534:1538	arg1	accessibility					1540:1552	Ready accessibility	1534:1552	Ready accessibility of 6dHepp	1534:1562	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	2	54	theme	jejuni	335:340	arg1	polysaccharides					351:365	The C. jejuni capsular polysaccharides	328:365	The C. jejuni capsular polysaccharides	328:365	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	2	54	theme	jejuni	335:340	arg1	antigens					451:458	potential antigens	441:458	potential antigens	441:458	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	1	55	theme	human	208:212	arg1	diseases					224:231	human diarrheal diseases	208:231	human diarrheal diseases	208:231	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	1	56	theme	diseases	224:231	arg1	cause					199:203	the leading cause	187:203	the leading cause of human diarrheal diseases	187:231	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	1	56	theme	diseases	224:231	arg1	jejuni					177:182	Campylobacter jejuni	163:182	Campylobacter jejuni	163:182	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	5	57	theme	strain	1135:1140	arg1	polysaccharide					1107:1120	the capsular polysaccharide	1094:1120	the capsular polysaccharide of C. jejuni strain CG8486	1094:1147	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	6	58	theme	assisted	1445:1452	arg1	glycosylation					1470:1482	anchimerically assisted stereoselective glycosylation	1430:1482	anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride	1430:1531	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	6	59	theme	-d-gulo-heptopyranoside	1371:1393	arg1	configuration					1341:1353	the C2 configuration	1334:1353	the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride	1334:1531	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	6	60	theme	1,2-trans	1361:1369	arg1	-d-gulo-heptopyranoside					1371:1393	β-(1,2-trans)-d-gulo-heptopyranoside	1358:1393	β-(1,2-trans)-d-gulo-heptopyranoside	1358:1393	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	5	61	theme	unique	996:1001	arg1	-β-d-GlcpNAc-					1053:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	6	62	theme	challenging	1272:1282	arg1	-d-ido-heptopyranoside					1295:1316	the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside	1254:1316	the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside	1254:1316	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	0	63	theme	Hexasaccharide	71:84	arg1	Assembly					57:64	Assembly	57:64	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.	0:161	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.					
34260212	5	64	theme	C.	1125:1126	arg1	strain					1135:1140	C. jejuni strain CG8486	1125:1147	C. jejuni strain CG8486	1125:1147	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	5	65	theme	protocol	971:978	arg1	application					951:961	the application	947:961	the application of this protocol	947:978	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	7	66	theme	synthetic	1672:1680	arg1	vaccines					1682:1689	synthetic vaccines	1672:1689	synthetic vaccines fighting Campylobacter infection	1672:1722	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	0	67	theme	jejuni	117:122	arg1	Polysaccharide					147:160	Campylobacter jejuni Strain CG8486 Capsular Polysaccharide	103:160	Campylobacter jejuni Strain CG8486 Capsular Polysaccharide	103:160	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.					
34260212	5	68	theme	-β-d-6didoHepp-	1033:1047	arg1	-β-d-GlcpNAc-					1053:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	0	69	theme	CG8486	131:136	arg1	Polysaccharide					147:160	Campylobacter jejuni Strain CG8486 Capsular Polysaccharide	103:160	Campylobacter jejuni Strain CG8486 Capsular Polysaccharide	103:160	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.					
34260212	5	70	theme	1→4	1049:1051	arg1	-β-d-GlcpNAc-					1053:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	4	71	theme	radical	821:827	arg1	fluorination					845:856	radical decarboxylative fluorination	821:856	radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides	821:924	The synthesis is achieved by a C1-to-C5 switch strategy relying on radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides.					
34260212	5	72	theme	composed	1018:1025	arg1	-β-d-GlcpNAc-					1053:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	2	73	theme	innovative	471:480	arg1	vaccines					513:520	innovative antibacterial glycoconjugation vaccines	471:520	innovative antibacterial glycoconjugation vaccines	471:520	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	2	74	theme	residues	410:417	arg1	existence					381:389	broad existence	375:389	broad existence of uncommon 6dHepp residues	375:417	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	0	75	theme	6-Deoxy-d-/l-Heptopyranosyl	18:44	arg1	Fluorides					46:54	Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides	13:54	Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides	13:54	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.					
34260212	1	76	theme	World	301:305	arg1	Organization					314:325	the World Health Organization	297:325	the World Health Organization	297:325	Campylobacter jejuni is the leading cause of human diarrheal diseases and has been designated as one of highly resistant pathogens by the World Health Organization.					
34260212	5	77	theme	→3	1030:1031	arg1	-β-d-GlcpNAc-					1053:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-	981:1065	To further showcase the application of this protocol, a structurally unique hexasaccharide composed of →3)-β-d-6didoHepp-(1→4)-β-d-GlcpNAc-(1→ units, corresponding to the capsular polysaccharide of C. jejuni strain CG8486 has been assembled for the first time.					
34260212	3	78	theme	d-/l-6dHepp	691:701	arg1	fluorides					703:711	uncommon d-/l-6dHepp fluorides	682:711	uncommon d-/l-6dHepp fluorides that have power as glycosylating agents	682:751	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
34260212	4	79	theme	switch	794:799	arg1	strategy					801:808	a C1-to-C5 switch strategy	783:808	a C1-to-C5 switch strategy relying on radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides	783:924	The synthesis is achieved by a C1-to-C5 switch strategy relying on radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides.					
34260212	2	80	theme	potential	441:449	arg1	polysaccharides					351:365	The C. jejuni capsular polysaccharides	328:365	The C. jejuni capsular polysaccharides	328:365	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	2	80	theme	potential	441:449	arg1	antigens					451:458	potential antigens	441:458	potential antigens	441:458	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	6	81	theme	fluoride	1524:1531	arg1	glycosylation					1470:1482	anchimerically assisted stereoselective glycosylation	1430:1482	anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride	1430:1531	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	7	82	theme	further	1649:1655	arg1	development					1657:1667	the further development	1645:1667	the further development of synthetic vaccines fighting Campylobacter infection	1645:1722	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	6	83	theme	protected	1504:1512	arg1	fluoride					1524:1531	the orthogonally protected 6dgulHepp fluoride	1487:1531	the orthogonally protected 6dgulHepp fluoride	1487:1531	The assembly is characterized by highly efficient construction of the synthetically challenging β-(1,2-cis)-d-ido-heptopyranoside by inversion of the C2 configuration of β-(1,2-trans)-d-gulo-heptopyranoside, which is conveniently obtained by anchimerically assisted stereoselective glycosylation of the orthogonally protected 6dgulHepp fluoride.					
34260212	2	84	theme	glycoconjugation	496:511	arg1	vaccines					513:520	innovative antibacterial glycoconjugation vaccines	471:520	innovative antibacterial glycoconjugation vaccines	471:520	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	0	85	dep	Synthesis	0:8	arg1	Assembly					57:64	Assembly	57:64	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.	0:161	Synthesis of Rare 6-Deoxy-d-/l-Heptopyranosyl Fluorides: Assembly of a Hexasaccharide Corresponding to Campylobacter jejuni Strain CG8486 Capsular Polysaccharide.					
34260212	7	86	theme	starting	1626:1633	arg1	glycans					1592:1598	the resulting glycans	1578:1598	the resulting glycans	1578:1598	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	7	86	theme	starting	1626:1633	arg1	point					1635:1639	a rational starting point	1615:1639	a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection	1615:1722	Ready accessibility of 6dHepp fluorides and the resulting glycans could serve as a rational starting point for the further development of synthetic vaccines fighting Campylobacter infection.					
34260212	2	87	theme	capsular	342:349	arg1	polysaccharides					351:365	The C. jejuni capsular polysaccharides	328:365	The C. jejuni capsular polysaccharides	328:365	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	2	87	theme	capsular	342:349	arg1	antigens					451:458	potential antigens	441:458	potential antigens	441:458	The C. jejuni capsular polysaccharides feature broad existence of uncommon 6dHepp residues and have proven to be potential antigens to develop innovative antibacterial glycoconjugation vaccines.					
34260212	4	88	theme	uronic	861:866	arg1	acids					868:872	uronic acids	861:872	uronic acids arising from readily available allyl d-C-glycosides	861:924	The synthesis is achieved by a C1-to-C5 switch strategy relying on radical decarboxylative fluorination of uronic acids arising from readily available allyl d-C-glycosides.					
34260212	3	89	theme	rare	568:571	arg1	architectures					580:592	rare 6dHepp architectures	568:592	rare 6dHepp architectures of importance	568:606	To address the lack of synthetic methods for rare 6dHepp architectures of importance, we herein describe a novel and efficient approach for the preparation of uncommon d-/l-6dHepp fluorides that have power as glycosylating agents.					
32573768	0	0	theme	transglycosylation	114:131	arg1	activity					133:140	mutant catalytic domain transglycosylation activity	90:140	mutant catalytic domain transglycosylation activity	90:140	Carbohydrate-binding domains facilitate efficient oligosaccharides synthesis by enhancing mutant catalytic domain transglycosylation activity.					
32573768	3	1	theme	diffusion	796:804	arg1	limitations					806:816	substrate diffusion limitations	786:816	substrate diffusion limitations	786:816	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	4	2	theme	CAZyme	1158:1163	arg1	chimeras					1165:1172	CBM-based CAZyme chimeras	1148:1172	CBM-based CAZyme chimeras	1148:1172	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	3	3	theme	substitution	917:928	arg1	mechanisms					930:939	any nucleophilic substitution mechanisms	900:939	any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step	900:985	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	0	4	theme	domain	107:112	arg1	activity					133:140	mutant catalytic domain transglycosylation activity	90:140	mutant catalytic domain transglycosylation activity	90:140	Carbohydrate-binding domains facilitate efficient oligosaccharides synthesis by enhancing mutant catalytic domain transglycosylation activity.					
32573768	2	5	theme	native	401:406	arg1	glycosyltransferases					408:427	native glycosyltransferases	401:427	native glycosyltransferases	401:427	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	3	6	theme	glycosyl	964:971	arg1	step					982:985	the actual glycosyl transfer step	953:985	the actual glycosyl transfer step	953:985	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	5	7	theme	structural	1348:1357	arg1	analysis					1359:1366	small-angle X-ray scattering structural analysis	1319:1366	small-angle X-ray scattering structural analysis along with detailed mutagenesis studies	1319:1406	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	4	8	theme	CBMs	1049:1052	arg1	participation					1032:1044	the direct participation	1021:1044	the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity	1021:1142	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	5	9	theme	understood	1264:1273	arg1	mechanism					1284:1292	this poorly understood reaction mechanism	1252:1292	this poorly understood reaction mechanism	1252:1292	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	6	10	theme	simple	1665:1670	arg1	monomers					1688:1695	simple activated sugar monomers	1665:1695	simple activated sugar monomers	1665:1695	In summary, our study provides a novel strategy for engineering similar CBM-based CAZyme chimeras for the synthesis of bespoke oligosaccharides using simple activated sugar monomers.					
32573768	2	11	theme	cellodextrins	339:351	arg1	synthesis					353:361	cellodextrins synthesis	339:361	cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates	339:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	3	12	theme	transfer	973:980	arg1	step					982:985	the actual glycosyl transfer step	953:985	the actual glycosyl transfer step	953:985	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	2	13	theme	bond	588:591	arg1	synthesis					593:601	glycosidic bond synthesis	577:601	glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates	577:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	3	14	theme	tethered	822:829	arg1	CDs					850:852	CDs	850:852	CDs	850:852	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	3	14	theme	tethered	822:829	arg1	domains					841:847	tethered catalytic domains	822:847	tethered catalytic domains (CDs)	822:853	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	4	15	theme	β-glucan	1089:1096	arg1	activity					1135:1142	β-glucan glycosidic bonds synthesis enhancing activity	1089:1142	β-glucan glycosidic bonds synthesis enhancing activity	1089:1142	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	3	16	theme	nucleophilic	904:915	arg1	mechanisms					930:939	any nucleophilic substitution mechanisms	900:939	any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step	900:985	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	2	17	theme	glycosidic	577:586	arg1	synthesis					593:601	glycosidic bond synthesis	577:601	glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates	577:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	3	18	theme	catalytic	831:839	arg1	CDs					850:852	CDs	850:852	CDs	850:852	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	3	18	theme	catalytic	831:839	arg1	domains					841:847	tethered catalytic domains	822:847	tethered catalytic domains (CDs)	822:853	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	6	19	theme	oligosaccharides	1642:1657	arg1	synthesis					1621:1629	the synthesis	1617:1629	the synthesis of bespoke oligosaccharides using simple activated sugar monomers	1617:1695	In summary, our study provides a novel strategy for engineering similar CBM-based CAZyme chimeras for the synthesis of bespoke oligosaccharides using simple activated sugar monomers.					
32573768	6	20	theme	CBM-based	1587:1595	arg1	chimeras					1604:1611	similar CBM-based CAZyme chimeras	1579:1611	similar CBM-based CAZyme chimeras	1579:1611	In summary, our study provides a novel strategy for engineering similar CBM-based CAZyme chimeras for the synthesis of bespoke oligosaccharides using simple activated sugar monomers.					
32573768	6	21	theme	activated	1672:1680	arg1	monomers					1688:1695	simple activated sugar monomers	1665:1695	simple activated sugar monomers	1665:1695	In summary, our study provides a novel strategy for engineering similar CBM-based CAZyme chimeras for the synthesis of bespoke oligosaccharides using simple activated sugar monomers.					
32573768	1	22	theme	promising	221:229	arg1	avenue					231:236	a promising avenue	219:236	a promising avenue for the synthesis of glycans like oligosaccharides	219:287	Chemoenzymatic approaches using carbohydrate-active enzymes (CAZymes) offer a promising avenue for the synthesis of glycans like oligosaccharides.					
32573768	4	23	theme	bonds	1109:1113	arg1	activity					1135:1142	β-glucan glycosidic bonds synthesis enhancing activity	1089:1142	β-glucan glycosidic bonds synthesis enhancing activity	1089:1142	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	5	24	theme	scattering	1337:1346	arg1	analysis					1359:1366	small-angle X-ray scattering structural analysis	1319:1366	small-angle X-ray scattering structural analysis along with detailed mutagenesis studies	1319:1406	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	2	25	theme	chemoenzymatic	314:327	arg1	route					329:333	a novel chemoenzymatic route	306:333	a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates	306:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	6	26	theme	CAZyme	1597:1602	arg1	chimeras					1604:1611	similar CBM-based CAZyme chimeras	1579:1611	similar CBM-based CAZyme chimeras	1579:1611	In summary, our study provides a novel strategy for engineering similar CBM-based CAZyme chimeras for the synthesis of bespoke oligosaccharides using simple activated sugar monomers.					
32573768	2	27	theme	carbohydrate-binding	515:534	arg1	CBMs					545:548	CBMs	545:548	CBMs	545:548	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	2	27	theme	carbohydrate-binding	515:534	arg1	modules					536:542	carbohydrate-binding modules	515:542	carbohydrate-binding modules (CBMs)	515:549	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	5	28	theme	X-ray	1331:1335	arg1	scattering					1337:1346	small-angle X-ray scattering	1319:1346	small-angle X-ray scattering structural analysis along with detailed mutagenesis studies	1319:1406	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	4	29	theme	reaction	1071:1078	arg1	step					1080:1083	the catalytic reaction step	1057:1083	the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity	1057:1142	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	2	30	theme	chimeric	375:382	arg1	CAZymes					384:390	chimeric CAZymes	375:390	chimeric CAZymes	375:390	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	5	31	theme	intradomain	1211:1221	arg1	interactions					1223:1234	Dynamic intradomain interactions	1203:1234	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism	1203:1292	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	2	32	theme	unprecedented	444:456	arg1	participation					458:470	the unprecedented participation	440:470	the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates	440:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	0	33	theme	Carbohydrate-binding	0:19	arg1	domains					21:27	Carbohydrate-binding domains	0:27	Carbohydrate-binding domains	0:27	Carbohydrate-binding domains facilitate efficient oligosaccharides synthesis by enhancing mutant catalytic domain transglycosylation activity.					
32573768	6	34	theme	sugar	1682:1686	arg1	monomers					1688:1695	simple activated sugar monomers	1665:1695	simple activated sugar monomers	1665:1695	In summary, our study provides a novel strategy for engineering similar CBM-based CAZyme chimeras for the synthesis of bespoke oligosaccharides using simple activated sugar monomers.					
32573768	4	35	theme	enhancing	1125:1133	arg1	activity					1135:1142	β-glucan glycosidic bonds synthesis enhancing activity	1089:1142	β-glucan glycosidic bonds synthesis enhancing activity	1089:1142	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	0	36	theme	efficient	40:48	arg1	synthesis					67:75	efficient oligosaccharides synthesis	40:75	efficient oligosaccharides synthesis	40:75	Carbohydrate-binding domains facilitate efficient oligosaccharides synthesis by enhancing mutant catalytic domain transglycosylation activity.					
32573768	1	37	theme	Chemoenzymatic	143:156	arg1	approaches					158:167	Chemoenzymatic approaches	143:167	Chemoenzymatic approaches using carbohydrate-active enzymes (CAZymes)	143:211	Chemoenzymatic approaches using carbohydrate-active enzymes (CAZymes) offer a promising avenue for the synthesis of glycans like oligosaccharides.					
32573768	5	38	theme	similar	1462:1468	arg1	mechanisms					1503:1512	similar transglycosylation-type reaction mechanisms	1462:1512	similar transglycosylation-type reaction mechanisms	1462:1512	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	2	39	theme	"	491:491	arg1	domain					505:510	a "non-catalytic" lectin-like domain	475:510	a "non-catalytic" lectin-like domain	475:510	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	2	40	theme	lectin-like	493:503	arg1	domain					505:510	a "non-catalytic" lectin-like domain	475:510	a "non-catalytic" lectin-like domain	475:510	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	5	41	theme	small-angle	1319:1329	arg1	scattering					1337:1346	small-angle X-ray scattering	1319:1346	small-angle X-ray scattering structural analysis along with detailed mutagenesis studies	1319:1406	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	2	42	theme	donor	623:627	arg1	sugars					629:634	β-cellobiosyl donor sugars	609:634	β-cellobiosyl donor sugars	609:634	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	2	42	theme	donor	623:627	arg1	substrates					649:658	activated substrates	639:658	activated substrates	639:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	4	43	from	participation	1032:1044	arg1	step					1080:1083	the catalytic reaction step	1057:1083	the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity	1057:1142	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	5	44	theme	reaction	1494:1501	arg1	mechanisms					1503:1512	similar transglycosylation-type reaction mechanisms	1462:1512	similar transglycosylation-type reaction mechanisms	1462:1512	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	5	45	theme	transglycosylation-type	1470:1492	arg1	mechanisms					1503:1512	similar transglycosylation-type reaction mechanisms	1462:1512	similar transglycosylation-type reaction mechanisms	1462:1512	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	2	46	theme	non-catalytic	478:490	arg1	domain					505:510	a "non-catalytic" lectin-like domain	475:510	a "non-catalytic" lectin-like domain	475:510	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	4	47	theme	glycosidic	1098:1107	arg1	activity					1135:1142	β-glucan glycosidic bonds synthesis enhancing activity	1089:1142	β-glucan glycosidic bonds synthesis enhancing activity	1089:1142	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	2	48	theme	akin	393:396	arg1	CAZymes					384:390	chimeric CAZymes	375:390	chimeric CAZymes	375:390	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	2	49	theme	β-cellobiosyl	609:621	arg1	sugars					629:634	β-cellobiosyl donor sugars	609:634	β-cellobiosyl donor sugars	609:634	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	2	49	theme	β-cellobiosyl	609:621	arg1	substrates					649:658	activated substrates	639:658	activated substrates	639:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	6	50	theme	bespoke	1634:1640	arg1	oligosaccharides					1642:1657	bespoke oligosaccharides	1634:1657	bespoke oligosaccharides using simple activated sugar monomers	1634:1695	In summary, our study provides a novel strategy for engineering similar CBM-based CAZyme chimeras for the synthesis of bespoke oligosaccharides using simple activated sugar monomers.					
32573768	4	51	theme	CBM-based	1148:1156	arg1	chimeras					1165:1172	CBM-based CAZyme chimeras	1148:1172	CBM-based CAZyme chimeras	1148:1172	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	1	52	theme	glycans	259:265	arg1	synthesis					246:254	the synthesis	242:254	the synthesis of glycans like oligosaccharides	242:287	Chemoenzymatic approaches using carbohydrate-active enzymes (CAZymes) offer a promising avenue for the synthesis of glycans like oligosaccharides.					
32573768	0	53	theme	oligosaccharides	50:65	arg1	synthesis					67:75	efficient oligosaccharides synthesis	40:75	efficient oligosaccharides synthesis	40:75	Carbohydrate-binding domains facilitate efficient oligosaccharides synthesis by enhancing mutant catalytic domain transglycosylation activity.					
32573768	5	54	theme	reaction	1275:1282	arg1	mechanism					1284:1292	this poorly understood reaction mechanism	1252:1292	this poorly understood reaction mechanism	1252:1292	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	4	55	theme	direct	1025:1030	arg1	participation					1032:1044	the direct participation	1021:1044	the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity	1021:1142	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	3	56	theme	substrate	702:710	arg1	role					722:725	a passive substrate targeting role	692:725	a passive substrate targeting role	692:725	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	2	57	theme	domain	505:510	arg1	participation					458:470	the unprecedented participation	440:470	the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates	440:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	4	58	theme	synthesis	1115:1123	arg1	activity					1135:1142	β-glucan glycosidic bonds synthesis enhancing activity	1089:1142	β-glucan glycosidic bonds synthesis enhancing activity	1089:1142	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	5	59	theme	Dynamic	1203:1209	arg1	interactions					1223:1234	Dynamic intradomain interactions	1203:1234	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism	1203:1292	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	2	60	theme	catalytic	558:566	arg1	step					568:571	the catalytic step	554:571	the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates	554:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	2	61	theme	modules	536:542	arg1	participation					458:470	the unprecedented participation	440:470	the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates	440:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	6	62	theme	similar	1579:1585	arg1	chimeras					1604:1611	similar CBM-based CAZyme chimeras	1579:1611	similar CBM-based CAZyme chimeras	1579:1611	In summary, our study provides a novel strategy for engineering similar CBM-based CAZyme chimeras for the synthesis of bespoke oligosaccharides using simple activated sugar monomers.					
32573768	3	63	theme	targeting	712:720	arg1	role					722:725	a passive substrate targeting role	692:725	a passive substrate targeting role	692:725	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	4	64	theme	catalytic	1061:1069	arg1	step					1080:1083	the catalytic reaction step	1057:1083	the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity	1057:1142	This study provides evidence for the direct participation of CBMs in the catalytic reaction step for β-glucan glycosidic bonds synthesis enhancing activity for CBM-based CAZyme chimeras by >140-fold over CDs alone.					
32573768	1	65	theme	carbohydrate-active	175:193	arg1	enzymes					195:201	carbohydrate-active enzymes	175:201	carbohydrate-active enzymes (CAZymes)	175:211	Chemoenzymatic approaches using carbohydrate-active enzymes (CAZymes) offer a promising avenue for the synthesis of glycans like oligosaccharides.					
32573768	1	65	theme	carbohydrate-active	175:193	arg1	CAZymes					204:210	CAZymes	204:210	CAZymes	204:210	Chemoenzymatic approaches using carbohydrate-active enzymes (CAZymes) offer a promising avenue for the synthesis of glycans like oligosaccharides.					
32573768	6	66	theme	novel	1548:1552	arg1	strategy					1554:1561	a novel strategy	1546:1561	a novel strategy for engineering similar CBM-based CAZyme chimeras for the synthesis of bespoke oligosaccharides using simple activated sugar monomers	1546:1695	In summary, our study provides a novel strategy for engineering similar CBM-based CAZyme chimeras for the synthesis of bespoke oligosaccharides using simple activated sugar monomers.					
32573768	2	67	theme	activated	639:647	arg1	sugars					629:634	β-cellobiosyl donor sugars	609:634	β-cellobiosyl donor sugars	609:634	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	2	67	theme	activated	639:647	arg1	substrates					649:658	activated substrates	639:658	activated substrates	639:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	5	68	theme	mechanisms	1503:1512	arg1	understanding					1445:1457	our current limited understanding	1425:1457	our current limited understanding of similar transglycosylation-type reaction mechanisms	1425:1512	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	2	69	theme	novel	308:312	arg1	route					329:333	a novel chemoenzymatic route	306:333	a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates	306:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	2	70	from	participation	458:470	arg1	step					568:571	the catalytic step	554:571	the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates	554:658	Here, we report a novel chemoenzymatic route for cellodextrins synthesis employed by chimeric CAZymes, akin to native glycosyltransferases, involving the unprecedented participation of a "non-catalytic" lectin-like domain or carbohydrate-binding modules (CBMs) in the catalytic step for glycosidic bond synthesis using β-cellobiosyl donor sugars as activated substrates.					
32573768	3	71	theme	actual	957:962	arg1	step					982:985	the actual glycosyl transfer step	953:985	the actual glycosyl transfer step	953:985	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	3	72	theme	enzymatic	730:738	arg1	reactions					754:762	enzymatic glycosylation reactions	730:762	enzymatic glycosylation reactions	730:762	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	3	73	theme	passive	694:700	arg1	role					722:725	a passive substrate targeting role	692:725	a passive substrate targeting role	692:725	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	5	74	dep	along	1368:1372	arg1	with					1374:1377	with	1374:1377	with	1374:1377	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	5	75	theme	limited	1437:1443	arg1	understanding					1445:1457	our current limited understanding	1425:1457	our current limited understanding of similar transglycosylation-type reaction mechanisms	1425:1512	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	5	76	theme	detailed	1379:1386	arg1	studies					1400:1406	detailed mutagenesis studies	1379:1406	small-angle X-ray scattering structural analysis along with detailed mutagenesis studies	1319:1406	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	0	77	theme	catalytic	97:105	arg1	activity					133:140	mutant catalytic domain transglycosylation activity	90:140	mutant catalytic domain transglycosylation activity	90:140	Carbohydrate-binding domains facilitate efficient oligosaccharides synthesis by enhancing mutant catalytic domain transglycosylation activity.					
32573768	3	78	theme	glycosylation	740:752	arg1	reactions					754:762	enzymatic glycosylation reactions	730:762	enzymatic glycosylation reactions	730:762	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
32573768	5	79	theme	current	1429:1435	arg1	understanding					1445:1457	our current limited understanding	1425:1457	our current limited understanding of similar transglycosylation-type reaction mechanisms	1425:1512	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	5	80	theme	mutagenesis	1388:1398	arg1	studies					1400:1406	detailed mutagenesis studies	1379:1406	small-angle X-ray scattering structural analysis along with detailed mutagenesis studies	1319:1406	Dynamic intradomain interactions that facilitate this poorly understood reaction mechanism were further revealed by small-angle X-ray scattering structural analysis along with detailed mutagenesis studies to shed light on our current limited understanding of similar transglycosylation-type reaction mechanisms.					
32573768	0	81	theme	mutant	90:95	arg1	activity					133:140	mutant catalytic domain transglycosylation activity	90:140	mutant catalytic domain transglycosylation activity	90:140	Carbohydrate-binding domains facilitate efficient oligosaccharides synthesis by enhancing mutant catalytic domain transglycosylation activity.					
32573768	3	82	theme	substrate	786:794	arg1	limitations					806:816	substrate diffusion limitations	786:816	substrate diffusion limitations	786:816	CBMs are often thought to play a passive substrate targeting role in enzymatic glycosylation reactions mostly via overcoming substrate diffusion limitations for tethered catalytic domains (CDs) but are not known to participate directly in any nucleophilic substitution mechanisms that impact the actual glycosyl transfer step.					
31829411	3	0	theme	N-glycans	966:974	arg1	sequence					976:983	the Fc N-glycans sequence	959:983	the Fc N-glycans sequence	959:983	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	1	1	theme	Fc	267:268	arg1	FcγRs					283:287	FcγRs	283:287	FcγRs	283:287	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	1	1	theme	Fc	267:268	arg1	receptors					272:280	membrane-bound Fc γ receptors	252:280	membrane-bound Fc γ receptors (FcγRs)	252:288	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	7	2	theme	glycosylated	1748:1759	arg1	FcγRs					1761:1765	N162 glycosylated FcγRs	1743:1765	N162 glycosylated FcγRs	1743:1765	Furthermore, structural analysis shows that the core-fucose position within the Fc core obstructs the access of N162 glycosylated FcγRs very much like a "door-stop," potentially decreasing the IgG/FcγR binding free energy.					
31829411	6	3	theme	α	1496:1496	arg1	arm					1504:1506	the extended sialylated α (1-3) arm	1472:1506	the extended sialylated α (1-3) arm	1472:1506	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	3	4	theme	observed	992:999	arg1	function					1006:1013	the observed IgG1 function	988:1013	the observed IgG1 function	988:1013	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	6	5	theme	extended	1476:1483	arg1	arm					1504:1506	the extended sialylated α (1-3) arm	1472:1506	the extended sialylated α (1-3) arm	1472:1506	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	5	6	theme	terminal	1363:1370	arg1	acid					1379:1382	a terminal sialic acid	1361:1382	a terminal sialic acid	1361:1382	At room temperature, the terminal galactose on the α (1-6) arm is restrained to the protein through a network of interactions that keep the arm outstretched; meanwhile, the α (1-3) arm extends toward the solvent where a terminal sialic acid remains fully accessible.					
31829411	1	7	theme	Fc	196:197	arg1	N-glycans					199:207	The immunoglobulin type G (IgG) Fc N-glycans	164:207	The immunoglobulin type G (IgG) Fc N-glycans	164:207	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	0	8	theme	sampling	135:142	arg1	dynamics					154:161	enhanced sampling molecular dynamics	126:161	enhanced sampling molecular dynamics	126:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	6	9	theme	fucose	1449:1454	arg1	presence					1432:1439	the presence	1428:1439	the presence of core fucose	1428:1454	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	4	10	theme	core	1117:1120	arg1	residues					1133:1140	their core trimannose residues	1111:1140	their core trimannose residues	1111:1140	Our results indicate that the two symmetrically opposed N-glycans interact extensively through their core trimannose residues.					
31829411	6	11	theme	conformational	1522:1535	arg1	propensity					1537:1546	its conformational propensity	1518:1546	its conformational propensity	1518:1546	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	2	12	theme	antibody-dependent	568:585	arg1	ADCC					610:613	ADCC	610:613	ADCC	610:613	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	2	12	theme	antibody-dependent	568:585	arg1	cytotoxicity					596:607	the antibody-dependent cellular cytotoxicity	564:607	the antibody-dependent cellular cytotoxicity (ADCC)	564:614	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	6	13	theme	Fc	1618:1619	arg1	dynamics					1621:1628	the Fc dynamics	1614:1628	the Fc dynamics	1614:1628	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	6	14	gly	sialylated	1485:1494	arg1	arm					1504:1506	the extended sialylated α (1-3) arm	1472:1506	the extended sialylated α (1-3) arm	1472:1506	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	7	15	theme	core-fucose	1679:1689	arg1	position					1691:1698	the core-fucose position	1675:1698	the core-fucose position within the Fc core	1675:1717	Furthermore, structural analysis shows that the core-fucose position within the Fc core obstructs the access of N162 glycosylated FcγRs very much like a "door-stop," potentially decreasing the IgG/FcγR binding free energy.					
31829411	5	16	theme	interactions	1256:1267	arg1	network					1245:1251	a network	1243:1251	a network of interactions that keep the arm outstretched	1243:1298	At room temperature, the terminal galactose on the α (1-6) arm is restrained to the protein through a network of interactions that keep the arm outstretched; meanwhile, the α (1-3) arm extends toward the solvent where a terminal sialic acid remains fully accessible.					
31829411	3	17	theme	atomistic	781:789	arg1	perspective					797:807	an atomistic level perspective	778:807	an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function	778:1013	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	3	18	theme	computer	835:842	arg1	simulations					844:854	enhanced sampling computer simulations	817:854	enhanced sampling computer simulations	817:854	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	8	19	theme	level-of-detail	1889:1903	arg1	framework					1905:1913	an atomistic level-of-detail framework	1876:1913	an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms	1876:1965	These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.					
31829411	1	20	theme	effector	318:325	arg1	function					327:334	the antibody's effector function	303:334	the antibody's effector function	303:334	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	1	21	theme	immunoglobulin	168:181	arg1	IgG					191:193	IgG	191:193	IgG	191:193	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	1	21	theme	immunoglobulin	168:181	arg1	G					188:188	immunoglobulin type G	168:188	The immunoglobulin type G (IgG) Fc N-glycans	164:207	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	6	22	dep	altering	1509:1516	arg1	enhancing					1604:1612	enhancing	1604:1612	significantly enhancing the Fc dynamics	1590:1628	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	0	23	from	perspective	13:23	arg1	cytotoxicity					56:67	antibody-dependent cellular cytotoxicity	28:67	antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics	28:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	3	24	theme	enhanced	817:824	arg1	simulations					844:854	enhanced sampling computer simulations	817:854	enhanced sampling computer simulations	817:854	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	6	25	theme	hindrance	1579:1587	arg1	consequence					1557:1567	a consequence	1555:1567	a consequence of steric hindrance	1555:1587	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	1	26	theme	G	188:188	arg1	N-glycans					199:207	The immunoglobulin type G (IgG) Fc N-glycans	164:207	The immunoglobulin type G (IgG) Fc N-glycans	164:207	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	0	27	theme	Fc	108:109	arg1	N-glycans					111:119	IgG1 Fc N-glycans	103:119	IgG1 Fc N-glycans from enhanced sampling molecular dynamics	103:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	1	28	theme	sequence-dependent	341:358	arg1	manner					360:365	a sequence-dependent manner	339:365	a sequence-dependent manner	339:365	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	0	29	gly	core-fucosylation	82:98	arg1	N-glycans					111:119	IgG1 Fc N-glycans	103:119	IgG1 Fc N-glycans from enhanced sampling molecular dynamics	103:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	2	30	theme	core	506:509	arg1	fucosylation					511:522	core fucosylation	506:522	core fucosylation	506:522	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	2	31	theme	sialylation	624:634	arg1	roles					417:421	the roles	413:421	the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation	413:747	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	8	32	theme	high	1933:1936	arg1	N-glycoforms					1954:1965	high potency IgG1 Fc N-glycoforms	1933:1965	high potency IgG1 Fc N-glycoforms	1933:1965	These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.					
31829411	2	33	theme	cellular	675:682	arg1	ADCC					698:701	ADCC	698:701	ADCC	698:701	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	2	33	theme	cellular	675:682	arg1	cytotoxicity					684:695	antibody-dependent cellular cytotoxicity	656:695	antibody-dependent cellular cytotoxicity (ADCC)	656:702	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	5	34	theme	α	1316:1316	arg1	arm					1324:1326	the α (1-3) arm	1312:1326	the α (1-3) arm	1312:1326	At room temperature, the terminal galactose on the α (1-6) arm is restrained to the protein through a network of interactions that keep the arm outstretched; meanwhile, the α (1-3) arm extends toward the solvent where a terminal sialic acid remains fully accessible.					
31829411	5	34	theme	α	1316:1316	arg1	meanwhile					1301:1309	meanwhile	1301:1309	meanwhile	1301:1309	At room temperature, the terminal galactose on the α (1-6) arm is restrained to the protein through a network of interactions that keep the arm outstretched; meanwhile, the α (1-3) arm extends toward the solvent where a terminal sialic acid remains fully accessible.					
31829411	8	35	theme	IgG1	1946:1949	arg1	N-glycoforms					1954:1965	high potency IgG1 Fc N-glycoforms	1933:1965	high potency IgG1 Fc N-glycoforms	1933:1965	These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.					
31829411	8	36	theme	N-glycoforms	1954:1965	arg1	design					1923:1928	the design	1919:1928	the design of high potency IgG1 Fc N-glycoforms	1919:1965	These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.					
31829411	7	37	theme	binding	1833:1839	arg1	energy					1846:1851	the IgG/FcγR binding free energy	1820:1851	the IgG/FcγR binding free energy	1820:1851	Furthermore, structural analysis shows that the core-fucose position within the Fc core obstructs the access of N162 glycosylated FcγRs very much like a "door-stop," potentially decreasing the IgG/FcγR binding free energy.					
31829411	2	38	theme	autoimmune	470:479	arg1	conditions					481:490	autoimmune conditions	470:490	autoimmune conditions	470:490	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	0	39	theme	antibody-dependent	28:45	arg1	cytotoxicity					56:67	antibody-dependent cellular cytotoxicity	28:67	antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics	28:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	3	40	theme	molecular	928:936	arg1	determinants					938:949	the molecular determinants	924:949	the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function	924:1013	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	1	41	with	interaction	235:245	arg1	FcγRs					283:287	FcγRs	283:287	FcγRs	283:287	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	1	41	with	interaction	235:245	arg1	receptors					272:280	membrane-bound Fc γ receptors	252:280	membrane-bound Fc γ receptors (FcγRs)	252:288	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	0	42	from	dynamics	154:161	arg1	N-glycans					111:119	IgG1 Fc N-glycans	103:119	IgG1 Fc N-glycans from enhanced sampling molecular dynamics	103:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	0	42	from	dynamics	154:161	arg1	core-fucosylation					82:98	core-fucosylation	82:98	core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics	82:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	3	43	theme	exchange	874:881	arg1	REMD					903:906	REMD	903:906	REMD	903:906	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	3	43	theme	exchange	874:881	arg1	dynamics					893:900	replica exchange molecular dynamics	866:900	replica exchange molecular dynamics (REMD)	866:907	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	3	44	theme	Fc	963:964	arg1	sequence					976:983	the Fc N-glycans sequence	959:983	the Fc N-glycans sequence	959:983	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	1	45	theme	membrane-bound	252:265	arg1	FcγRs					283:287	FcγRs	283:287	FcγRs	283:287	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	1	45	theme	membrane-bound	252:265	arg1	receptors					272:280	membrane-bound Fc γ receptors	252:280	membrane-bound Fc γ receptors (FcγRs)	252:288	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	3	46	theme	IgG1	1001:1004	arg1	function					1006:1013	the observed IgG1 function	988:1013	the observed IgG1 function	988:1013	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	1	47	theme	γ	270:270	arg1	FcγRs					283:287	FcγRs	283:287	FcγRs	283:287	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	1	47	theme	γ	270:270	arg1	receptors					272:280	membrane-bound Fc γ receptors	252:280	membrane-bound Fc γ receptors (FcγRs)	252:288	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	2	48	theme	cellular	587:594	arg1	ADCC					610:613	ADCC	610:613	ADCC	610:613	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	2	48	theme	cellular	587:594	arg1	cytotoxicity					596:607	the antibody-dependent cellular cytotoxicity	564:607	the antibody-dependent cellular cytotoxicity (ADCC)	564:614	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	6	49	theme	sialylated	1485:1494	arg1	arm					1504:1506	the extended sialylated α (1-3) arm	1472:1506	the extended sialylated α (1-3) arm	1472:1506	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	7	50	theme	Fc	1711:1712	arg1	core					1714:1717	the Fc core	1707:1717	the Fc core	1707:1717	Furthermore, structural analysis shows that the core-fucose position within the Fc core obstructs the access of N162 glycosylated FcγRs very much like a "door-stop," potentially decreasing the IgG/FcγR binding free energy.					
31829411	2	51	theme	core-fucosylation	731:747	arg1	context					720:726	the context	716:726	the context of core-fucosylation	716:747	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	5	52	from	galactose	1177:1185	arg1	arm					1202:1204	the α (1-6) arm	1190:1204	the α (1-6) arm	1190:1204	At room temperature, the terminal galactose on the α (1-6) arm is restrained to the protein through a network of interactions that keep the arm outstretched; meanwhile, the α (1-3) arm extends toward the solvent where a terminal sialic acid remains fully accessible.					
31829411	0	53	theme	molecular	144:152	arg1	dynamics					154:161	enhanced sampling molecular dynamics	126:161	enhanced sampling molecular dynamics	126:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	5	54	theme	sialic	1372:1377	arg1	acid					1379:1382	a terminal sialic acid	1361:1382	a terminal sialic acid	1361:1382	At room temperature, the terminal galactose on the α (1-6) arm is restrained to the protein through a network of interactions that keep the arm outstretched; meanwhile, the α (1-3) arm extends toward the solvent where a terminal sialic acid remains fully accessible.					
31829411	7	55	gly	glycosylated	1748:1759	arg1	FcγRs					1761:1765	N162 glycosylated FcγRs	1743:1765	N162 glycosylated FcγRs	1743:1765	Furthermore, structural analysis shows that the core-fucose position within the Fc core obstructs the access of N162 glycosylated FcγRs very much like a "door-stop," potentially decreasing the IgG/FcγR binding free energy.					
31829411	2	56	from	respect	401:407	arg1	interesting					381:391	interesting	381:391	interesting	381:391	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	4	57	theme	trimannose	1122:1131	arg1	residues					1133:1140	their core trimannose residues	1111:1140	their core trimannose residues	1111:1140	Our results indicate that the two symmetrically opposed N-glycans interact extensively through their core trimannose residues.					
31829411	6	58	theme	core	1444:1447	arg1	fucose					1449:1454	core fucose	1444:1454	core fucose	1444:1454	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	0	59	from	core-fucosylation	82:98	arg1	dynamics					154:161	enhanced sampling molecular dynamics	126:161	enhanced sampling molecular dynamics	126:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	8	60	theme	atomistic	1879:1887	arg1	framework					1905:1913	an atomistic level-of-detail framework	1876:1913	an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms	1876:1965	These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.					
31829411	3	61	theme	level	791:795	arg1	perspective					797:807	an atomistic level perspective	778:807	an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function	778:1013	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	7	62	theme	structural	1644:1653	arg1	analysis					1655:1662	structural analysis	1644:1662	structural analysis	1644:1662	Furthermore, structural analysis shows that the core-fucose position within the Fc core obstructs the access of N162 glycosylated FcγRs very much like a "door-stop," potentially decreasing the IgG/FcγR binding free energy.					
31829411	2	63	theme	fucosylation	511:522	arg1	roles					417:421	the roles	413:421	the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation	413:747	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	0	64	theme	IgG1	103:106	arg1	Fc					108:109	IgG1 Fc	103:109	IgG1 Fc N-glycans from enhanced sampling molecular dynamics	103:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	1	65	theme	type	183:186	arg1	IgG					191:193	IgG	191:193	IgG	191:193	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	1	65	theme	type	183:186	arg1	G					188:188	immunoglobulin type G	168:188	The immunoglobulin type G (IgG) Fc N-glycans	164:207	The immunoglobulin type G (IgG) Fc N-glycans are known to modulate the interaction with membrane-bound Fc γ receptors (FcγRs), fine-tuning the antibody's effector function in a sequence-dependent manner.					
31829411	3	66	theme	molecular	883:891	arg1	REMD					903:906	REMD	903:906	REMD	903:906	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	3	66	theme	molecular	883:891	arg1	dynamics					893:900	replica exchange molecular dynamics	866:900	replica exchange molecular dynamics (REMD)	866:907	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	3	67	theme	sampling	826:833	arg1	simulations					844:854	enhanced sampling computer simulations	817:854	enhanced sampling computer simulations	817:854	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	0	68	theme	N-glycans	111:119	arg1	core-fucosylation					82:98	core-fucosylation	82:98	core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics	82:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	5	69	theme	room	1146:1149	arg1	temperature					1151:1161	room temperature	1146:1161	room temperature	1146:1161	At room temperature, the terminal galactose on the α (1-6) arm is restrained to the protein through a network of interactions that keep the arm outstretched; meanwhile, the α (1-3) arm extends toward the solvent where a terminal sialic acid remains fully accessible.					
31829411	6	70	theme	steric	1572:1577	arg1	hindrance					1579:1587	steric hindrance	1572:1587	steric hindrance	1572:1587	We also find that the presence of core fucose interferes with the extended sialylated α (1-3) arm, altering its conformational propensity and as a consequence of steric hindrance, significantly enhancing the Fc dynamics.					
31829411	3	71	theme	replica	866:872	arg1	REMD					903:906	REMD	903:906	REMD	903:906	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	3	71	theme	replica	866:872	arg1	dynamics					893:900	replica exchange molecular dynamics	866:900	replica exchange molecular dynamics (REMD)	866:907	In this article, we provide an atomistic level perspective through enhanced sampling computer simulations, based on replica exchange molecular dynamics (REMD), to understand the molecular determinants linking the Fc N-glycans sequence to the observed IgG1 function.					
31829411	7	72	theme	FcγRs	1761:1765	arg1	access					1733:1738	the access	1729:1738	the access of N162 glycosylated FcγRs	1729:1765	Furthermore, structural analysis shows that the core-fucose position within the Fc core obstructs the access of N162 glycosylated FcγRs very much like a "door-stop," potentially decreasing the IgG/FcγR binding free energy.					
31829411	8	73	gly	N-glycoforms	1954:1965	arg1	IgG1					1946:1949	high potency IgG1 Fc N-glycoforms	1933:1965	high potency IgG1 Fc N-glycoforms	1933:1965	These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.					
31829411	8	73	gly	N-glycoforms	1954:1965	arg1	Fc					1951:1952	high potency IgG1 Fc N-glycoforms	1933:1965	high potency IgG1 Fc N-glycoforms	1933:1965	These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.					
31829411	8	73	gly	N-glycoforms	1954:1965	arg1	potency					1938:1944	high potency IgG1 Fc N-glycoforms	1933:1965	high potency IgG1 Fc N-glycoforms	1933:1965	These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.					
31829411	0	74	theme	enhanced	126:133	arg1	dynamics					154:161	enhanced sampling molecular dynamics	126:161	enhanced sampling molecular dynamics	126:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	2	75	attach	linked	460:465	arg1	aging					496:500	aging	496:500	aging	496:500	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	2	75	attach	linked	460:465	arg1	conditions					481:490	autoimmune conditions	470:490	autoimmune conditions	470:490	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	2	75	attach	linked	460:465	arg2	levels					449:454	levels	449:454	levels	449:454	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	8	76	theme	potency	1938:1944	arg1	N-glycoforms					1954:1965	high potency IgG1 Fc N-glycoforms	1933:1965	high potency IgG1 Fc N-glycoforms	1933:1965	These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.					
31829411	0	77	theme	atomistic	3:11	arg1	perspective					13:23	An atomistic perspective	0:23	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.	0:162	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	7	78	theme	free	1841:1844	arg1	energy					1846:1851	the IgG/FcγR binding free energy	1820:1851	the IgG/FcγR binding free energy	1820:1851	Furthermore, structural analysis shows that the core-fucose position within the Fc core obstructs the access of N162 glycosylated FcγRs very much like a "door-stop," potentially decreasing the IgG/FcγR binding free energy.					
31829411	2	79	theme	antibody-dependent	656:673	arg1	ADCC					698:701	ADCC	698:701	ADCC	698:701	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	2	79	theme	antibody-dependent	656:673	arg1	cytotoxicity					684:695	antibody-dependent cellular cytotoxicity	656:695	antibody-dependent cellular cytotoxicity (ADCC)	656:702	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	7	80	theme	IgG/FcγR	1824:1831	arg1	energy					1846:1851	the IgG/FcγR binding free energy	1820:1851	the IgG/FcγR binding free energy	1820:1851	Furthermore, structural analysis shows that the core-fucose position within the Fc core obstructs the access of N162 glycosylated FcγRs very much like a "door-stop," potentially decreasing the IgG/FcγR binding free energy.					
31829411	2	81	theme	galactosylation	426:440	arg1	roles					417:421	the roles	413:421	the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation	413:747	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	5	82	theme	outstretched	1287:1298	arg1	arm					1283:1285	the arm outstretched	1279:1298	the arm outstretched	1279:1298	At room temperature, the terminal galactose on the α (1-6) arm is restrained to the protein through a network of interactions that keep the arm outstretched; meanwhile, the α (1-3) arm extends toward the solvent where a terminal sialic acid remains fully accessible.					
31829411	0	83	theme	cellular	47:54	arg1	cytotoxicity					56:67	antibody-dependent cellular cytotoxicity	28:67	antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics	28:161	An atomistic perspective on antibody-dependent cellular cytotoxicity quenching by core-fucosylation of IgG1 Fc N-glycans from enhanced sampling molecular dynamics.					
31829411	5	84	theme	α	1194:1194	arg1	arm					1202:1204	the α (1-6) arm	1190:1204	the α (1-6) arm	1190:1204	At room temperature, the terminal galactose on the α (1-6) arm is restrained to the protein through a network of interactions that keep the arm outstretched; meanwhile, the α (1-3) arm extends toward the solvent where a terminal sialic acid remains fully accessible.					
31829411	2	85	from	interesting	381:391	arg1	respect					401:407	this respect	396:407	this respect	396:407	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	2	86	dep	roles	417:421	arg1	interesting					381:391	interesting	381:391	interesting	381:391	Particularly interesting in this respect are the roles of galactosylation, which levels are linked to autoimmune conditions and aging, of core fucosylation, which is known to reduce significantly the antibody-dependent cellular cytotoxicity (ADCC), and of sialylation, which also reduces antibody-dependent cellular cytotoxicity (ADCC) but only in the context of core-fucosylation.					
31829411	5	87	theme	terminal	1168:1175	arg1	galactose					1177:1185	the terminal galactose	1164:1185	the terminal galactose on the α (1-6) arm	1164:1204	At room temperature, the terminal galactose on the α (1-6) arm is restrained to the protein through a network of interactions that keep the arm outstretched; meanwhile, the α (1-3) arm extends toward the solvent where a terminal sialic acid remains fully accessible.					
31829411	4	88	theme	opposed	1064:1070	arg1	N-glycans					1072:1080	the two symmetrically opposed N-glycans	1042:1080	the two symmetrically opposed N-glycans	1042:1080	Our results indicate that the two symmetrically opposed N-glycans interact extensively through their core trimannose residues.					
31829411	8	89	theme	Fc	1951:1952	arg1	N-glycoforms					1954:1965	high potency IgG1 Fc N-glycoforms	1933:1965	high potency IgG1 Fc N-glycoforms	1933:1965	These results provide an atomistic level-of-detail framework for the design of high potency IgG1 Fc N-glycoforms.					
32940477	1	0	theme	N-linked	167:174	arg1	glycans					176:182	N-linked glycans	167:182	N-linked glycans differing in structure from those found in all other organisms	167:245	Chloroviruses produce a capsid protein containing N-linked glycans differing in structure from those found in all other organisms.					
32940477	0	1	theme	"	96:96	arg1	Approach					107:114	a "Counterclockwise" Assembly Approach	77:114	a "Counterclockwise" Assembly Approach	77:114	Synthesis of a Highly Branched Nonasaccharide Chlorella Virus N-Glycan Using a "Counterclockwise" Assembly Approach.					
32940477	2	2	theme	"	292:292	arg1	residue					301:307	a core "hyper-branched" fucose residue	270:307	a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated	270:353	These species feature a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated.					
32940477	3	3	theme	nonasaccharide	387:400	arg1	synthesis					372:380	the synthesis	368:380	the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported	368:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	0	4	theme	Counterclockwise	80:95	arg1	Approach					107:114	a "Counterclockwise" Assembly Approach	77:114	a "Counterclockwise" Assembly Approach	77:114	Synthesis of a Highly Branched Nonasaccharide Chlorella Virus N-Glycan Using a "Counterclockwise" Assembly Approach.					
32940477	2	5	theme	hyper-branched	278:291	arg1	residue					301:307	a core "hyper-branched" fucose residue	270:307	a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated	270:353	These species feature a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated.					
32940477	1	6	contain	containing	156:165	arg1	protein					148:154	a capsid protein	139:154	a capsid protein containing N-linked glycans differing in structure from those found in all other organisms	139:245	Chloroviruses produce a capsid protein containing N-linked glycans differing in structure from those found in all other organisms.					
32940477	1	6	contain	containing	156:165	arg2	glycans					176:182	N-linked glycans	167:182	N-linked glycans differing in structure from those found in all other organisms	167:245	Chloroviruses produce a capsid protein containing N-linked glycans differing in structure from those found in all other organisms.					
32940477	3	7	theme	"	523:523	arg1	strategy					525:532	a "counterclockwise" strategy	504:532	a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue	504:678	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	0	8	theme	Assembly	98:105	arg1	Approach					107:114	a "Counterclockwise" Assembly Approach	77:114	a "Counterclockwise" Assembly Approach	77:114	Synthesis of a Highly Branched Nonasaccharide Chlorella Virus N-Glycan Using a "Counterclockwise" Assembly Approach.					
32940477	3	9	theme	Paramecium	407:416	arg1	virus					437:441	Paramecium bursaria chlorella virus 1	407:443	Paramecium bursaria chlorella virus 1	407:443	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	9	theme	Paramecium	407:416	arg1	one					446:448	one	446:448	one	446:448	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	9	theme	Paramecium	407:416	arg1	N-glycans					478:486	most complex chlorovirus N-glycans	453:486	most complex chlorovirus N-glycans reported	453:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	2	10	theme	core	272:275	arg1	residue					301:307	a core "hyper-branched" fucose residue	270:307	a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated	270:353	These species feature a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated.					
32940477	3	11	from	synthesis	372:380	arg1	virus					437:441	Paramecium bursaria chlorella virus 1	407:443	Paramecium bursaria chlorella virus 1	407:443	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	11	from	synthesis	372:380	arg1	one					446:448	one	446:448	one	446:448	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	11	from	synthesis	372:380	arg1	N-glycans					478:486	most complex chlorovirus N-glycans	453:486	most complex chlorovirus N-glycans reported	453:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	12	theme	chlorella	427:435	arg1	virus					437:441	Paramecium bursaria chlorella virus 1	407:443	Paramecium bursaria chlorella virus 1	407:443	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	12	theme	chlorella	427:435	arg1	one					446:448	one	446:448	one	446:448	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	12	theme	chlorella	427:435	arg1	N-glycans					478:486	most complex chlorovirus N-glycans	453:486	most complex chlorovirus N-glycans reported	453:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	13	theme	trisaccharide	571:583	arg1	addition					559:566	the sequential addition	544:566	the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue	544:678	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	14	theme	sequential	548:557	arg1	addition					559:566	the sequential addition	544:566	the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue	544:678	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	15	contain	containing	645:654	arg1	trisaccharide					631:643	a trisaccharide	629:643	a trisaccharide containing the core fucose residue	629:678	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	15	contain	containing	645:654	arg2	residue					672:678	the core fucose residue	656:678	the core fucose residue	656:678	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	16	from	virus	437:441	arg1	synthesis					372:380	the synthesis	368:380	the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported	368:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	16	from	virus	437:441	arg1	nonasaccharide					387:400	a nonasaccharide	385:400	a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported	385:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	1	17	link	N-linked	167:174	arg1	glycans					176:182	N-linked glycans	167:182	N-linked glycans differing in structure from those found in all other organisms	167:245	Chloroviruses produce a capsid protein containing N-linked glycans differing in structure from those found in all other organisms.					
32940477	2	18	theme	fucose	294:299	arg1	residue					301:307	a core "hyper-branched" fucose residue	270:307	a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated	270:353	These species feature a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated.					
32940477	3	19	theme	complex	458:464	arg1	N-glycans					478:486	most complex chlorovirus N-glycans	453:486	most complex chlorovirus N-glycans reported	453:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	0	20	theme	Nonasaccharide	31:44	arg1	Virus					56:60	a Highly Branched Nonasaccharide Chlorella Virus	13:60	a Highly Branched Nonasaccharide Chlorella Virus N-Glycan	13:69	Synthesis of a Highly Branched Nonasaccharide Chlorella Virus N-Glycan Using a "Counterclockwise" Assembly Approach.					
32940477	2	21	gly	glycosylated	342:353	arg1	group					333:337	every hydroxyl group	318:337	every hydroxyl group	318:337	These species feature a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated.					
32940477	2	21	gly	glycosylated	342:353	arg1	residue					301:307	a core "hyper-branched" fucose residue	270:307	a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated	270:353	These species feature a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated.					
32940477	2	21	gly	glycosylated	342:353	arg2	group					333:337	every hydroxyl group	318:337	every hydroxyl group	318:337	These species feature a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated.					
32940477	3	22	theme	core	660:663	arg1	residue					672:678	the core fucose residue	656:678	the core fucose residue	656:678	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	0	23	theme	Branched	22:29	arg1	Virus					56:60	a Highly Branched Nonasaccharide Chlorella Virus	13:60	a Highly Branched Nonasaccharide Chlorella Virus N-Glycan	13:69	Synthesis of a Highly Branched Nonasaccharide Chlorella Virus N-Glycan Using a "Counterclockwise" Assembly Approach.					
32940477	3	24	theme	bursaria	418:425	arg1	virus					437:441	Paramecium bursaria chlorella virus 1	407:443	Paramecium bursaria chlorella virus 1	407:443	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	24	theme	bursaria	418:425	arg1	one					446:448	one	446:448	one	446:448	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	24	theme	bursaria	418:425	arg1	N-glycans					478:486	most complex chlorovirus N-glycans	453:486	most complex chlorovirus N-glycans reported	453:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	0	25	theme	Virus	56:60	arg1	N-Glycan					62:69	a Highly Branched Nonasaccharide Chlorella Virus N-Glycan	13:69	a Highly Branched Nonasaccharide Chlorella Virus N-Glycan	13:69	Synthesis of a Highly Branched Nonasaccharide Chlorella Virus N-Glycan Using a "Counterclockwise" Assembly Approach.					
32940477	0	26	theme	Chlorella	46:54	arg1	Virus					56:60	a Highly Branched Nonasaccharide Chlorella Virus	13:60	a Highly Branched Nonasaccharide Chlorella Virus N-Glycan	13:69	Synthesis of a Highly Branched Nonasaccharide Chlorella Virus N-Glycan Using a "Counterclockwise" Assembly Approach.					
32940477	3	27	theme	chlorovirus	466:476	arg1	N-glycans					478:486	most complex chlorovirus N-glycans	453:486	most complex chlorovirus N-glycans reported	453:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	28	theme	disaccharide	586:597	arg1	addition					559:566	the sequential addition	544:566	the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue	544:678	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	2	29	theme	hydroxyl	324:331	arg1	group					333:337	every hydroxyl group	318:337	every hydroxyl group	318:337	These species feature a core "hyper-branched" fucose residue in which every hydroxyl group is glycosylated.					
32940477	3	30	theme	fucose	665:670	arg1	residue					672:678	the core fucose residue	656:678	the core fucose residue	656:678	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	31	theme	N-glycans	478:486	arg1	N-glycans					478:486	most complex chlorovirus N-glycans	453:486	most complex chlorovirus N-glycans reported	453:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	31	theme	N-glycans	478:486	arg1	one					446:448	one	446:448	one	446:448	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	31	theme	N-glycans	478:486	arg1	virus					437:441	Paramecium bursaria chlorella virus 1	407:443	Paramecium bursaria chlorella virus 1	407:443	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	1	32	theme	other	231:235	arg1	organisms					237:245	all other organisms	227:245	all other organisms	227:245	Chloroviruses produce a capsid protein containing N-linked glycans differing in structure from those found in all other organisms.					
32940477	1	33	located	found	218:222	arg1	organisms					237:245	all other organisms	227:245	all other organisms	227:245	Chloroviruses produce a capsid protein containing N-linked glycans differing in structure from those found in all other organisms.					
32940477	1	33	located	found	218:222	arg2	those					212:216	those	212:216	those	212:216	Chloroviruses produce a capsid protein containing N-linked glycans differing in structure from those found in all other organisms.					
32940477	3	34	theme	monosaccharide	604:617	arg1	motifs					619:624	monosaccharide motifs	604:624	monosaccharide motifs	604:624	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	35	theme	most	453:456	arg1	N-glycans					478:486	most complex chlorovirus N-glycans	453:486	most complex chlorovirus N-glycans reported	453:495	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	1	36	theme	capsid	141:146	arg1	protein					148:154	a capsid protein	139:154	a capsid protein containing N-linked glycans differing in structure from those found in all other organisms	139:245	Chloroviruses produce a capsid protein containing N-linked glycans differing in structure from those found in all other organisms.					
32940477	0	37	theme	N-Glycan	62:69	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a Highly Branched Nonasaccharide Chlorella Virus N-Glycan	0:69	Synthesis of a Highly Branched Nonasaccharide Chlorella Virus N-Glycan Using a "Counterclockwise" Assembly Approach.					
32940477	3	38	theme	motifs	619:624	arg1	addition					559:566	the sequential addition	544:566	the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue	544:678	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32940477	3	39	theme	counterclockwise	507:522	arg1	strategy					525:532	a "counterclockwise" strategy	504:532	a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue	504:678	We describe the synthesis of a nonasaccharide from Paramecium bursaria chlorella virus 1, one of most complex chlorovirus N-glycans reported, using a "counterclockwise" strategy involving the sequential addition of trisaccharide, disaccharide, and monosaccharide motifs to a trisaccharide containing the core fucose residue.					
32212661	0	0	theme	Glycosyl	86:93	arg1	Donors					95:100	Bimodal Glycosyl Donors	78:100	Bimodal Glycosyl Donors	78:100	Unified Strategy toward Stereocontrolled Assembly of Various Glucans Based on Bimodal Glycosyl Donors.					
32212661	1	1	theme	glycosidic	312:321	arg1	linkages					323:330	glycosidic linkages	312:330	glycosidic linkages	312:330	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	0	2	theme	Bimodal	78:84	arg1	Donors					95:100	Bimodal Glycosyl Donors	78:100	Bimodal Glycosyl Donors	78:100	Unified Strategy toward Stereocontrolled Assembly of Various Glucans Based on Bimodal Glycosyl Donors.					
32212661	3	3	theme	α-	679:680	arg1	synthesis					661:669	stereocontrolled synthesis	644:669	stereocontrolled synthesis of both α- and β-glycosides	644:697	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	3	4	theme	glucans	540:546	arg1	types					531:535	all types	527:535	all types of glucans	527:546	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	2	5	theme	unified	376:382	arg1	strategy					384:391	the unified strategy	372:391	the unified strategy for the assembly of pure glucans	372:424	Because of their structural diversity, the unified strategy for the assembly of pure glucans is yet to be developed.					
32212661	1	6	from	plants	264:269	arg1	present					223:229	present	223:229	present	223:229	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	2	7	theme	glucans	418:424	arg1	assembly					401:408	the assembly	397:408	the assembly of pure glucans	397:424	Because of their structural diversity, the unified strategy for the assembly of pure glucans is yet to be developed.					
32212661	1	8	from	mammals	251:257	arg1	present					223:229	present	223:229	present	223:229	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	9	with	plants	264:269	arg1	linkages					323:330	glycosidic linkages	312:330	glycosidic linkages	312:330	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	9	with	plants	264:269	arg1	configurations					293:306	various anomeric configurations	276:306	various anomeric configurations	276:306	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	10	theme	important	170:178	arg1	products					188:195	the biologically important natural products	153:195	the biologically important natural products	153:195	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	0	11	theme	Unified	0:6	arg1	Strategy					8:15	Unified Strategy	0:15	Unified Strategy toward Stereocontrolled Assembly of Various Glucans	0:67	Unified Strategy toward Stereocontrolled Assembly of Various Glucans Based on Bimodal Glycosyl Donors.					
32212661	1	12	with	mammals	251:257	arg1	linkages					323:330	glycosidic linkages	312:330	glycosidic linkages	312:330	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	12	with	mammals	251:257	arg1	configurations					293:306	various anomeric configurations	276:306	various anomeric configurations	276:306	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	3	13	theme	types	531:535	arg1	construction					511:522	construction	511:522	construction of all types of glucans	511:546	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	1	14	theme	natural	180:186	arg1	products					188:195	the biologically important natural products	153:195	the biologically important natural products	153:195	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	0	15	theme	Stereocontrolled	24:39	arg1	Assembly					41:48	Stereocontrolled Assembly	24:48	Stereocontrolled Assembly of Various Glucans	24:67	Unified Strategy toward Stereocontrolled Assembly of Various Glucans Based on Bimodal Glycosyl Donors.					
32212661	3	16	theme	reaction	712:719	arg1	conditions					721:730	reaction conditions	712:730	reaction conditions	712:730	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	1	17	from	bacteria	241:248	arg1	present					223:229	present	223:229	present	223:229	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	18	attach	present	223:229	arg1	mammals					251:257	mammals	251:257	mammals	251:257	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	18	attach	present	223:229	arg2	Polymers					103:110	Polymers	103:110	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans	103:210	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	18	attach	present	223:229	arg1	bacteria					241:248	bacteria	241:248	bacteria	241:248	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	18	attach	present	223:229	arg1	fungi					234:238	fungi	234:238	fungi	234:238	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	18	attach	present	223:229	arg1	plants					264:269	plants	264:269	plants with various anomeric configurations and glycosidic linkages	264:330	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	19	dep	abundant	133:140	arg1	the					124:126	the	124:126	the	124:126	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	20	theme	products	188:195	arg1	glucose					115:121	glucose	115:121	glucose	115:121	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	20	theme	products	188:195	arg1	one					146:148	one	146:148	one	146:148	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	20	theme	products	188:195	arg1	products					188:195	the biologically important natural products	153:195	the biologically important natural products	153:195	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	3	21	theme	β-glycosides	686:697	arg1	synthesis					661:669	stereocontrolled synthesis	644:669	stereocontrolled synthesis of both α- and β-glycosides	644:697	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	3	22	theme	C2-o-TsNHbenzyl	601:615	arg1	TAB					624:626	TAB	624:626	TAB	624:626	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	3	22	theme	C2-o-TsNHbenzyl	601:615	arg1	ether					617:621	C2-o-TsNHbenzyl ether	601:621	C2-o-TsNHbenzyl ether (TAB)	601:627	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	3	23	theme	general	472:478	arg1	applicable					497:506	applicable	497:506	applicable	497:506	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	3	23	theme	general	472:478	arg1	strategy					480:487	a general strategy	470:487	a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions	470:730	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	1	24	from	fungi	234:238	arg1	present					223:229	present	223:229	present	223:229	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	25	theme	named	198:202	arg1	glucans					204:210	glucose, the most abundant and one of the biologically important natural products, named glucans	115:210	glucose, the most abundant and one of the biologically important natural products, named glucans	115:210	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	2	26	theme	pure	413:416	arg1	glucans					418:424	pure glucans	413:424	pure glucans	413:424	Because of their structural diversity, the unified strategy for the assembly of pure glucans is yet to be developed.					
32212661	1	27	with	bacteria	241:248	arg1	linkages					323:330	glycosidic linkages	312:330	glycosidic linkages	312:330	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	27	with	bacteria	241:248	arg1	configurations					293:306	various anomeric configurations	276:306	various anomeric configurations	276:306	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	28	theme	glucans	204:210	arg1	Polymers					103:110	Polymers	103:110	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans	103:210	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	0	29	theme	Glucans	61:67	arg1	Assembly					41:48	Stereocontrolled Assembly	24:48	Stereocontrolled Assembly of Various Glucans	24:67	Unified Strategy toward Stereocontrolled Assembly of Various Glucans Based on Bimodal Glycosyl Donors.					
32212661	1	30	theme	various	276:282	arg1	configurations					293:306	various anomeric configurations	276:306	various anomeric configurations	276:306	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	31	from	present	223:229	arg1	mammals					251:257	mammals	251:257	mammals	251:257	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	31	from	present	223:229	arg1	plants					264:269	plants	264:269	plants with various anomeric configurations and glycosidic linkages	264:330	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	31	from	present	223:229	arg1	bacteria					241:248	bacteria	241:248	bacteria	241:248	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	31	from	present	223:229	arg1	fungi					234:238	fungi	234:238	fungi	234:238	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	32	theme	glucose	115:121	arg1	glucans					204:210	glucose, the most abundant and one of the biologically important natural products, named glucans	115:210	glucose, the most abundant and one of the biologically important natural products, named glucans	115:210	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	0	33	theme	Various	53:59	arg1	Glucans					61:67	Various Glucans	53:67	Various Glucans	53:67	Unified Strategy toward Stereocontrolled Assembly of Various Glucans Based on Bimodal Glycosyl Donors.					
32212661	1	34	theme	anomeric	284:291	arg1	configurations					293:306	various anomeric configurations	276:306	various anomeric configurations	276:306	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	35	located	present	223:229	arg1	mammals					251:257	mammals	251:257	mammals	251:257	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	35	located	present	223:229	arg2	Polymers					103:110	Polymers	103:110	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans	103:210	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	35	located	present	223:229	arg1	bacteria					241:248	bacteria	241:248	bacteria	241:248	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	35	located	present	223:229	arg1	fungi					234:238	fungi	234:238	fungi	234:238	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	35	located	present	223:229	arg1	plants					264:269	plants	264:269	plants with various anomeric configurations and glycosidic linkages	264:330	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	36	with	fungi	234:238	arg1	linkages					323:330	glycosidic linkages	312:330	glycosidic linkages	312:330	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	1	36	with	fungi	234:238	arg1	configurations					293:306	various anomeric configurations	276:306	various anomeric configurations	276:306	Polymers of glucose, the most abundant and one of the biologically important natural products, named glucans are widely present in fungi, bacteria, mammals, and plants with various anomeric configurations and glycosidic linkages.					
32212661	3	37	theme	glycosyl	572:579	arg1	donor					581:585	a bimodal glycosyl donor	562:585	a bimodal glycosyl donor	562:585	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	2	38	theme	structural	350:359	arg1	diversity					361:369	their structural diversity	344:369	their structural diversity	344:369	Because of their structural diversity, the unified strategy for the assembly of pure glucans is yet to be developed.					
32212661	3	39	theme	stereocontrolled	644:659	arg1	synthesis					661:669	stereocontrolled synthesis	644:669	stereocontrolled synthesis of both α- and β-glycosides	644:697	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
32212661	3	40	theme	bimodal	564:570	arg1	donor					581:585	a bimodal glycosyl donor	562:585	a bimodal glycosyl donor	562:585	Herein, we describe a general strategy that is applicable to construction of all types of glucans by exploiting a bimodal glycosyl donor equipped with C2-o-TsNHbenzyl ether (TAB), which enables stereocontrolled synthesis of both α- and β-glycosides by switching reaction conditions.					
33991006	0	0	theme	Fragments	79:87	arg1	Synthesis					12:20	(Automated) Synthesis	0:20	(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments	0:87	(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments.					
33991006	5	1	theme	possible	751:758	arg1	modifications					774:786	all possible glycosylation modifications	747:786	all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs	747:891	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	6	2	theme	trityl	1005:1010	arg1	group					1012:1016	a dimethoxy trityl group	993:1016	a dimethoxy trityl group	993:1016	DNA-type chemistry, employing ribitol phosphoramidite building blocks, protected with a dimethoxy trityl group, was used to efficiently generate a library of WTA-hexamers.					
33991006	9	3	theme	specific	1553:1560	arg1	location					1562:1569	the specific location	1549:1569	the specific location of the GlcNAc modifications on the WTA-chains	1549:1615	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	9	4	theme	magnetic	1444:1451	arg1	beads					1453:1457	WTA-functionalized magnetic beads	1425:1457	WTA-functionalized magnetic beads	1425:1457	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	6	5	theme	building	961:968	arg1	blocks					970:975	ribitol phosphoramidite building blocks	937:975	ribitol phosphoramidite building blocks	937:975	DNA-type chemistry, employing ribitol phosphoramidite building blocks, protected with a dimethoxy trityl group, was used to efficiently generate a library of WTA-hexamers.					
33991006	0	6	theme	Acid	74:77	arg1	Fragments					79:87	Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments	25:87	Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments	25:87	(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments.					
33991006	9	7	from	modifications	1585:1597	arg1	WTA-chains					1606:1615	the WTA-chains	1602:1615	the WTA-chains	1602:1615	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	7	8	theme	Automated	1079:1087	arg1	syntheses					1101:1109	Automated solid phase syntheses	1079:1109	Automated solid phase syntheses	1079:1109	Automated solid phase syntheses were used to assemble a WTA-dodecamer and glycosylated WTA-hexamer.					
33991006	5	9	from	C3	860:861	arg1	β-GlcNAc					839:846	β-GlcNAc	839:846	α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3	789:861	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	3	10	theme	host	498:501	arg1	system					510:515	the host immune system	494:515	the host immune system	494:515	These WTA-modifications play an important role in shaping the interactions of WTA with the host immune system.					
33991006	6	11	theme	phosphoramidite	945:959	arg1	blocks					970:975	ribitol phosphoramidite building blocks	937:975	ribitol phosphoramidite building blocks	937:975	DNA-type chemistry, employing ribitol phosphoramidite building blocks, protected with a dimethoxy trityl group, was used to efficiently generate a library of WTA-hexamers.					
33991006	7	12	theme	glycosylated	1153:1164	arg1	WTA-hexamer					1166:1176	a WTA-dodecamer and glycosylated WTA-hexamer	1133:1176	a WTA-dodecamer and glycosylated WTA-hexamer	1133:1176	Automated solid phase syntheses were used to assemble a WTA-dodecamer and glycosylated WTA-hexamer.					
33991006	3	13	theme	immune	503:508	arg1	system					510:515	the host immune system	494:515	the host immune system	494:515	These WTA-modifications play an important role in shaping the interactions of WTA with the host immune system.					
33991006	6	14	theme	ribitol	937:943	arg1	blocks					970:975	ribitol phosphoramidite building blocks	937:975	ribitol phosphoramidite building blocks	937:975	DNA-type chemistry, employing ribitol phosphoramidite building blocks, protected with a dimethoxy trityl group, was used to efficiently generate a library of WTA-hexamers.					
33991006	5	15	dep	chemistry	677:685	arg1	assemble					690:697	assemble	690:697	to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs,	687:892	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	5	16	from	C4	810:811	arg1	α-GlcNAc					789:796	α-GlcNAc	789:796	α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3	789:861	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	7	17	gly	glycosylated	1153:1164	arg1	WTA-hexamer					1166:1176	a WTA-dodecamer and glycosylated WTA-hexamer	1133:1176	a WTA-dodecamer and glycosylated WTA-hexamer	1133:1176	Automated solid phase syntheses were used to assemble a WTA-dodecamer and glycosylated WTA-hexamer.					
33991006	8	18	theme	diagnostic	1237:1246	arg1	signals					1248:1254	diagnostic signals	1237:1254	diagnostic signals	1237:1254	The synthetic fragments have been fully characterized and diagnostic signals were identified to discriminate the different glycosylation patterns.					
33991006	5	19	theme	WTA-fragments	718:730	arg1	library					707:713	a broad library	699:713	a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs,	699:892	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	9	20	theme	antibodies	1516:1525	arg1	importance					1535:1544	the importance	1531:1544	the importance of the specific location of the GlcNAc modifications on the WTA-chains	1531:1615	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	9	20	theme	antibodies	1516:1525	arg1	specificity					1482:1492	the binding specificity	1470:1492	the binding specificity of these WTA-specific antibodies	1470:1525	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	3	21	with	interactions	469:480	arg1	system					510:515	the host immune system	494:515	the host immune system	494:515	These WTA-modifications play an important role in shaping the interactions of WTA with the host immune system.					
33991006	4	22	theme	well-defined	600:611	arg1	molecules					617:625	pure and well-defined WTA molecules	591:625	pure and well-defined WTA molecules	591:625	Due to the structural heterogeneity of WTAs, it is impossible to isolate pure and well-defined WTA molecules from bacterial sources.					
33991006	9	23	theme	GlcNAc	1578:1583	arg1	modifications					1585:1597	the GlcNAc modifications	1574:1597	the GlcNAc modifications on the WTA-chains	1574:1615	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	9	24	theme	modifications	1585:1597	arg1	location					1562:1569	the specific location	1549:1569	the specific location of the GlcNAc modifications on the WTA-chains	1549:1615	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	1	25	theme	cell	149:152	arg1	wall					154:157	the cell wall	145:157	the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus	145:224	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	8	26	theme	synthetic	1183:1191	arg1	fragments					1193:1201	The synthetic fragments	1179:1201	The synthetic fragments	1179:1201	The synthetic fragments have been fully characterized and diagnostic signals were identified to discriminate the different glycosylation patterns.					
33991006	9	27	theme	WTA-functionalized	1425:1442	arg1	beads					1453:1457	WTA-functionalized magnetic beads	1425:1457	WTA-functionalized magnetic beads	1425:1457	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	2	28	theme	phosphate	266:274	arg1	residues					283:290	ribitol phosphate (RboP) residues	258:290	ribitol phosphate (RboP) residues that are decorated with d-alanine and N-acetyl-d-glucosamine (GlcNAc) modifications, in a seemingly random manner	258:404	WTAs are composed of repeating ribitol phosphate (RboP) residues that are decorated with d-alanine and N-acetyl-d-glucosamine (GlcNAc) modifications, in a seemingly random manner.					
33991006	1	29	theme	wall	154:157	arg1	components					131:140	important components	121:140	important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus	121:224	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	1	29	theme	wall	154:157	arg1	acids					104:108	Wall teichoic acids	90:108	Wall teichoic acids (WTAs)	90:115	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	0	30	theme	Automated	1:9	arg1	Synthesis					12:20	(Automated) Synthesis	0:20	(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments	0:87	(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments.					
33991006	5	31	theme	glycosylation	760:772	arg1	modifications					774:786	all possible glycosylation modifications	747:786	all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs	747:891	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	5	32	dep	modifications	774:786	arg1	α-GlcNAc					789:796	α-GlcNAc	789:796	α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3	789:861	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	4	33	theme	bacterial	632:640	arg1	sources					642:648	bacterial sources	632:648	bacterial sources	632:648	Due to the structural heterogeneity of WTAs, it is impossible to isolate pure and well-defined WTA molecules from bacterial sources.					
33991006	2	34	dep	d-alanine	316:324	arg1	modifications					362:374	modifications	362:374	modifications	362:374	WTAs are composed of repeating ribitol phosphate (RboP) residues that are decorated with d-alanine and N-acetyl-d-glucosamine (GlcNAc) modifications, in a seemingly random manner.					
33991006	6	35	theme	DNA-type	907:914	arg1	chemistry					916:924	DNA-type chemistry	907:924	DNA-type chemistry	907:924	DNA-type chemistry, employing ribitol phosphoramidite building blocks, protected with a dimethoxy trityl group, was used to efficiently generate a library of WTA-hexamers.					
33991006	5	36	theme	RboP	805:808	arg1	C4					810:811	the RboP C4	801:811	the RboP C4	801:811	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	6	37	used	used	1023:1026	arg2	chemistry					916:924	DNA-type chemistry	907:924	DNA-type chemistry	907:924	DNA-type chemistry, employing ribitol phosphoramidite building blocks, protected with a dimethoxy trityl group, was used to efficiently generate a library of WTA-hexamers.					
33991006	1	38	theme	Wall	90:93	arg1	components					131:140	important components	121:140	important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus	121:224	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	1	38	theme	Wall	90:93	arg1	WTAs					111:114	WTAs	111:114	WTAs	111:114	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	1	38	theme	Wall	90:93	arg1	acids					104:108	Wall teichoic acids	90:108	Wall teichoic acids (WTAs)	90:115	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	9	39	theme	binding	1474:1480	arg1	specificity					1482:1492	the binding specificity	1470:1492	the binding specificity of these WTA-specific antibodies	1470:1525	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	9	40	used	used	1372:1375	arg2	WTA-fragments					1353:1365	The different glycosylated WTA-fragments	1326:1365	The different glycosylated WTA-fragments	1326:1365	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	4	41	theme	structural	529:538	arg1	heterogeneity					540:552	the structural heterogeneity	525:552	the structural heterogeneity of WTAs	525:560	Due to the structural heterogeneity of WTAs, it is impossible to isolate pure and well-defined WTA molecules from bacterial sources.					
33991006	1	42	theme	teichoic	95:102	arg1	components					131:140	important components	121:140	important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus	121:224	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	1	42	theme	teichoic	95:102	arg1	WTAs					111:114	WTAs	111:114	WTAs	111:114	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	1	42	theme	teichoic	95:102	arg1	acids					104:108	Wall teichoic acids	90:108	Wall teichoic acids (WTAs)	90:115	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	1	43	theme	opportunistic	166:178	arg1	bacterium					194:202	the opportunistic Gram-positive bacterium	162:202	the opportunistic Gram-positive bacterium Staphylococcus aureus	162:224	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	4	44	theme	WTAs	557:560	arg1	heterogeneity					540:552	the structural heterogeneity	525:552	the structural heterogeneity of WTAs	525:560	Due to the structural heterogeneity of WTAs, it is impossible to isolate pure and well-defined WTA molecules from bacterial sources.					
33991006	7	45	used	used	1116:1119	arg2	syntheses					1101:1109	Automated solid phase syntheses	1079:1109	Automated solid phase syntheses	1079:1109	Automated solid phase syntheses were used to assemble a WTA-dodecamer and glycosylated WTA-hexamer.					
33991006	9	46	theme	WTA-specific	1503:1514	arg1	antibodies					1516:1525	these WTA-specific antibodies	1497:1525	these WTA-specific antibodies	1497:1525	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	9	47	theme	location	1562:1569	arg1	importance					1535:1544	the importance	1531:1544	the importance of the specific location of the GlcNAc modifications on the WTA-chains	1531:1615	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	9	47	theme	location	1562:1569	arg1	specificity					1482:1492	the binding specificity	1470:1492	the binding specificity of these WTA-specific antibodies	1470:1525	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	1	48	theme	Gram-positive	180:192	arg1	bacterium					194:202	the opportunistic Gram-positive bacterium	162:202	the opportunistic Gram-positive bacterium Staphylococcus aureus	162:224	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	0	49	theme	Well-defined	25:36	arg1	Fragments					79:87	Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments	25:87	Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments	25:87	(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments.					
33991006	6	50	theme	dimethoxy	995:1003	arg1	group					1012:1016	a dimethoxy trityl group	993:1016	a dimethoxy trityl group	993:1016	DNA-type chemistry, employing ribitol phosphoramidite building blocks, protected with a dimethoxy trityl group, was used to efficiently generate a library of WTA-hexamers.					
33991006	1	51	theme	bacterium	194:202	arg1	wall					154:157	the cell wall	145:157	the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus	145:224	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	5	52	theme	broad	701:705	arg1	library					707:713	a broad library	699:713	a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs,	699:892	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	8	53	theme	different	1292:1300	arg1	patterns					1316:1323	the different glycosylation patterns	1288:1323	the different glycosylation patterns	1288:1323	The synthetic fragments have been fully characterized and diagnostic signals were identified to discriminate the different glycosylation patterns.					
33991006	0	54	dep	Staphylococcus	38:51	arg1	Aureus					53:58	Aureus	53:58	Aureus	53:58	(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments.					
33991006	2	55	theme	random	392:397	arg1	manner					399:404	a seemingly random manner	380:404	a seemingly random manner	380:404	WTAs are composed of repeating ribitol phosphate (RboP) residues that are decorated with d-alanine and N-acetyl-d-glucosamine (GlcNAc) modifications, in a seemingly random manner.					
33991006	7	56	theme	solid	1089:1093	arg1	syntheses					1101:1109	Automated solid phase syntheses	1079:1109	Automated solid phase syntheses	1079:1109	Automated solid phase syntheses were used to assemble a WTA-dodecamer and glycosylated WTA-hexamer.					
33991006	9	57	theme	different	1330:1338	arg1	WTA-fragments					1353:1365	The different glycosylated WTA-fragments	1326:1365	The different glycosylated WTA-fragments	1326:1365	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	0	58	dep	Well-defined	25:36	arg1	Staphylococcus					38:51	Staphylococcus	38:51	Staphylococcus	38:51	(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments.					
33991006	4	59	gly	heterogeneity	540:552	arg1	WTAs					557:560	WTAs	557:560	WTAs	557:560	Due to the structural heterogeneity of WTAs, it is impossible to isolate pure and well-defined WTA molecules from bacterial sources.					
33991006	3	60	theme	WTA	485:487	arg1	interactions					469:480	the interactions	465:480	the interactions of WTA with the host immune system	465:515	These WTA-modifications play an important role in shaping the interactions of WTA with the host immune system.					
33991006	7	61	theme	phase	1095:1099	arg1	syntheses					1101:1109	Automated solid phase syntheses	1079:1109	Automated solid phase syntheses	1079:1109	Automated solid phase syntheses were used to assemble a WTA-dodecamer and glycosylated WTA-hexamer.					
33991006	9	62	from	WTA-chains	1606:1615	arg1	location					1562:1569	the specific location	1549:1569	the specific location of the GlcNAc modifications on the WTA-chains	1549:1615	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	5	63	theme	synthetic	667:675	arg1	chemistry					677:685	synthetic chemistry	667:685	synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs,	667:892	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	2	64	theme	ribitol	258:264	arg1	RboP					277:280	RboP	277:280	RboP	277:280	WTAs are composed of repeating ribitol phosphate (RboP) residues that are decorated with d-alanine and N-acetyl-d-glucosamine (GlcNAc) modifications, in a seemingly random manner.					
33991006	2	64	theme	ribitol	258:264	arg1	phosphate					266:274	ribitol phosphate	258:274	ribitol phosphate (RboP) residues that are decorated with d-alanine and N-acetyl-d-glucosamine (GlcNAc) modifications, in a seemingly random manner	258:404	WTAs are composed of repeating ribitol phosphate (RboP) residues that are decorated with d-alanine and N-acetyl-d-glucosamine (GlcNAc) modifications, in a seemingly random manner.					
33991006	9	65	theme	glycosylated	1340:1351	arg1	WTA-fragments					1353:1365	The different glycosylated WTA-fragments	1326:1365	The different glycosylated WTA-fragments	1326:1365	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	5	66	theme	S.	878:879	arg1	WTAs					888:891	S. aureus WTAs	878:891	S. aureus WTAs	878:891	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	9	67	from	location	1562:1569	arg1	WTA-chains					1606:1615	the WTA-chains	1602:1615	the WTA-chains	1602:1615	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	9	68	gly	glycosylated	1340:1351	arg1	WTA-fragments					1353:1365	The different glycosylated WTA-fragments	1326:1365	The different glycosylated WTA-fragments	1326:1365	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	5	69	dep	α-GlcNAc	789:796	arg1	β-GlcNAc					814:821	β-GlcNAc	814:821	α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3	789:861	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	5	69	dep	α-GlcNAc	789:796	arg1	β-GlcNAc					839:846	β-GlcNAc	839:846	α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3	789:861	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	8	70	theme	glycosylation	1302:1314	arg1	patterns					1316:1323	the different glycosylation patterns	1288:1323	the different glycosylation patterns	1288:1323	The synthetic fragments have been fully characterized and diagnostic signals were identified to discriminate the different glycosylation patterns.					
33991006	5	71	theme	aureus	881:886	arg1	WTAs					888:891	S. aureus WTAs	878:891	S. aureus WTAs	878:891	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	6	72	theme	WTA-hexamers	1065:1076	arg1	library					1054:1060	a library	1052:1060	a library of WTA-hexamers	1052:1076	DNA-type chemistry, employing ribitol phosphoramidite building blocks, protected with a dimethoxy trityl group, was used to efficiently generate a library of WTA-hexamers.					
33991006	0	73	theme	Teichoic	65:72	arg1	Fragments					79:87	Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments	25:87	Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments	25:87	(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments.					
33991006	5	74	theme	RboP	830:833	arg1	C4					835:836	the RboP C4	826:836	the RboP C4	826:836	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	4	75	theme	WTA	613:615	arg1	molecules					617:625	pure and well-defined WTA molecules	591:625	pure and well-defined WTA molecules	591:625	Due to the structural heterogeneity of WTAs, it is impossible to isolate pure and well-defined WTA molecules from bacterial sources.					
33991006	1	76	theme	important	121:129	arg1	components					131:140	important components	121:140	important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus	121:224	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	1	76	theme	important	121:129	arg1	acids					104:108	Wall teichoic acids	90:108	Wall teichoic acids (WTAs)	90:115	Wall teichoic acids (WTAs) are important components of the cell wall of the opportunistic Gram-positive bacterium Staphylococcus aureus.					
33991006	3	77	theme	important	439:447	arg1	role					449:452	an important role	436:452	an important role	436:452	These WTA-modifications play an important role in shaping the interactions of WTA with the host immune system.					
33991006	9	78	theme	monoclonal	1397:1406	arg1	antibodies					1408:1417	monoclonal antibodies	1397:1417	monoclonal antibodies using WTA-functionalized magnetic beads	1397:1457	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	0	79	theme	Wall	60:63	arg1	Fragments					79:87	Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments	25:87	Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments	25:87	(Automated) Synthesis of Well-defined Staphylococcus Aureus Wall Teichoic Acid Fragments.					
33991006	7	80	theme	WTA-dodecamer	1135:1147	arg1	WTA-hexamer					1166:1176	a WTA-dodecamer and glycosylated WTA-hexamer	1133:1176	a WTA-dodecamer and glycosylated WTA-hexamer	1133:1176	Automated solid phase syntheses were used to assemble a WTA-dodecamer and glycosylated WTA-hexamer.					
33991006	9	81	theme	antibodies	1408:1417	arg1	binding					1386:1392	binding	1386:1392	binding of monoclonal antibodies using WTA-functionalized magnetic beads	1386:1457	The different glycosylated WTA-fragments were used to probe binding of monoclonal antibodies using WTA-functionalized magnetic beads, revealing the binding specificity of these WTA-specific antibodies and the importance of the specific location of the GlcNAc modifications on the WTA-chains.					
33991006	5	82	from	C4	835:836	arg1	β-GlcNAc					814:821	β-GlcNAc	814:821	α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3	789:861	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	5	83	theme	RboP	855:858	arg1	C3					860:861	the RboP C3	851:861	the RboP C3	851:861	Therefore, here synthetic chemistry to assemble a broad library of WTA-fragments, incorporating all possible glycosylation modifications (α-GlcNAc at the RboP C4; β-GlcNAc at the RboP C4; β-GlcNAc at the RboP C3) described for S. aureus WTAs, is reported.					
33991006	4	84	theme	pure	591:594	arg1	molecules					617:625	pure and well-defined WTA molecules	591:625	pure and well-defined WTA molecules	591:625	Due to the structural heterogeneity of WTAs, it is impossible to isolate pure and well-defined WTA molecules from bacterial sources.					
32871472	4	0	theme	potent	481:486	arg1	activity					498:505	a potent antitumor activity	479:505	a potent antitumor activity in experimental models	479:528	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	14	1	from	cells	1665:1669	arg1	forms					1647:1651	the different forms	1633:1651	the different forms	1633:1651	Our modeling study suggests that the drug can target efficiently the different forms of PD-L1 in cells, glycosylated, ubiquitinated or not.					
32871472	12	2	theme	drug-stabilized	1306:1320	arg1	dimer					1330:1334	the drug-stabilized protein dimer	1302:1334	the drug-stabilized protein dimer	1302:1334	The interface of the drug-stabilized protein dimer is unaffected by the glycosylation or ubiquitination.					
32871472	2	3	used	used	263:266	arg2	antibodies					224:233	Monoclonal antibodies	213:233	Monoclonal antibodies targeting PD-1 or PD-L1	213:257	Monoclonal antibodies targeting PD-1 or PD-L1 are used to treat different types of solid tumors and lymphoma.					
32871472	15	4	theme	protein	1799:1805	arg1	complexes					1807:1815	other PD-L1 protein complexes	1787:1815	other PD-L1 protein complexes	1787:1815	These models of N-glycosylated and ubiquitinated PD-L1 will be useful to study other PD-L1 protein complexes.					
32871472	5	5	gly	N-glycosylation	571:585	arg2	N192					596:599	N192	596:599	N192	596:599	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	5	gly	N-glycosylation	571:585	arg1	N200					602:605	N200	602:605	N200	602:605	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	5	gly	N-glycosylation	571:585	arg1	N219					611:614	N219	611:614	N219	611:614	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	5	gly	N-glycosylation	571:585	arg1	K178					645:648	K178	645:648	K178	645:648	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	5	gly	N-glycosylation	571:585	arg1	N35					591:593	N35	591:593	N35	591:593	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	5	gly	N-glycosylation	571:585	arg2	K178					645:648	K178	645:648	K178	645:648	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	5	gly	N-glycosylation	571:585	arg2	N200					602:605	N200	602:605	N200	602:605	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	5	gly	N-glycosylation	571:585	arg1	N192					596:599	N192	596:599	N192	596:599	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	5	gly	N-glycosylation	571:585	arg2	N35					591:593	N35	591:593	N35	591:593	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	5	gly	N-glycosylation	571:585	arg2	N219					611:614	N219	611:614	N219	611:614	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	5	gly	N-glycosylation	571:585	arg1	PD-L1					654:658	PD-L1	654:658	PD-L1	654:658	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	4	6	from	activity	498:505	arg1	models					523:528	experimental models	510:528	experimental models	510:528	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	4	7	theme	lead	385:388	arg1	BMS-202					420:426	the biphenyl drug BMS-202	402:426	the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models	402:528	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	4	7	theme	lead	385:388	arg1	compound					390:397	The lead compound	381:397	The lead compound	381:397	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	5	8	from	N35	591:593	arg1	N-glycosylation					571:585	N-glycosylation	571:585	N-glycosylation (at N35, N192, N200 and N219)	571:615	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	8	from	N35	591:593	arg1	effect					561:566	the effect	557:566	the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202	557:690	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	8	from	N35	591:593	arg1	mono-ubiquitination					621:639	mono-ubiquitination	621:639	mono-ubiquitination (at K178)	621:649	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	10	9	theme	PD-L1	1161:1165	arg1	dimer					1167:1171	a PD-L1 dimer	1159:1171	a PD-L1 dimer	1159:1171	In both cases, BMS-202 was bound to the protein interface, stabilizing a PD-L1 dimer.					
32871472	0	10	theme	molecular	90:98	arg1	study					109:113	A molecular modeling study	88:113	BMS-202: A molecular modeling study	79:113	N-glycosylation and ubiquitinylation of PD-L1 do not restrict interaction with BMS-202: A molecular modeling study.					
32871472	5	11	from	N192	596:599	arg1	N-glycosylation					571:585	N-glycosylation	571:585	N-glycosylation (at N35, N192, N200 and N219)	571:615	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	11	from	N192	596:599	arg1	effect					561:566	the effect	557:566	the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202	557:690	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	11	from	N192	596:599	arg1	mono-ubiquitination					621:639	mono-ubiquitination	621:639	mono-ubiquitination (at K178)	621:649	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	12	theme	mono-ubiquitination	621:639	arg1	effect					561:566	the effect	557:566	the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202	557:690	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	13	13	theme	drug	1546:1549	arg1	process					1559:1565	the drug binding process	1542:1565	the drug binding process	1542:1565	Calculations of the binding energies indicated that the glycosylation slightly reduces the stability of the drug-protein complexes but does not prevent the drug binding process.					
32871472	8	14	theme	ubiquitin	1013:1021	arg1	residues					1023:1030	4 ubiquitin residues	1011:1030	4 ubiquitin residues	1011:1030	Model 1 refers to glycoPD-L1 bearing 16 N-glycans, with or without 4 ubiquitin residues.					
32871472	7	15	theme	fucosylated	891:901	arg1	N-glycan					916:923	a fucosylated bi-antennary N-glycan	889:923	a fucosylated bi-antennary N-glycan	889:923	The structures were glycosylated, with a fucosylated bi-antennary N-glycan and ubiquitinated.					
32871472	5	16	theme	molecular	695:703	arg1	modeling					705:712	molecular modeling	695:712	molecular modeling	695:712	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	14	17	theme	modeling	1572:1579	arg1	study					1581:1585	Our modeling study	1568:1585	Our modeling study	1568:1585	Our modeling study suggests that the drug can target efficiently the different forms of PD-L1 in cells, glycosylated, ubiquitinated or not.					
32871472	13	18	theme	binding	1551:1557	arg1	process					1559:1565	the drug binding process	1542:1565	the drug binding process	1542:1565	Calculations of the binding energies indicated that the glycosylation slightly reduces the stability of the drug-protein complexes but does not prevent the drug binding process.					
32871472	2	19	theme	tumors	302:307	arg1	types					287:291	different types	277:291	different types of solid tumors and lymphoma	277:320	Monoclonal antibodies targeting PD-1 or PD-L1 are used to treat different types of solid tumors and lymphoma.					
32871472	3	20	theme	PD-L1-binding	323:335	arg1	molecules					343:351	PD-L1-binding small molecules	323:351	PD-L1-binding small molecules	323:351	PD-L1-binding small molecules are also actively searched.					
32871472	13	21	theme	binding	1410:1416	arg1	energies					1418:1425	the binding energies	1406:1425	the binding energies	1406:1425	Calculations of the binding energies indicated that the glycosylation slightly reduces the stability of the drug-protein complexes but does not prevent the drug binding process.					
32871472	11	22	theme	drug-protein	1259:1270	arg1	recognition					1272:1282	the drug-protein recognition	1255:1282	the drug-protein recognition	1255:1282	The incorporation of the N-glycans or the ubiquitins did not significantly alter the drug-protein recognition.					
32871472	0	23	with	interaction	62:72	arg1	BMS-202					79:85	BMS-202	79:85	BMS-202: A molecular modeling study	79:113	N-glycosylation and ubiquitinylation of PD-L1 do not restrict interaction with BMS-202: A molecular modeling study.					
32871472	13	24	theme	energies	1418:1425	arg1	Calculations					1390:1401	Calculations	1390:1401	Calculations of the binding energies	1390:1425	Calculations of the binding energies indicated that the glycosylation slightly reduces the stability of the drug-protein complexes but does not prevent the drug binding process.					
32871472	1	25	theme	PD-1/L1	162:168	arg1	checkpoint					171:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	1	25	theme	PD-1/L1	162:168	arg1	target					193:198	a major target	185:198	a major target in oncology	185:210	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	0	26	theme	modeling	100:107	arg1	study					109:113	A molecular modeling study	88:113	BMS-202: A molecular modeling study	79:113	N-glycosylation and ubiquitinylation of PD-L1 do not restrict interaction with BMS-202: A molecular modeling study.					
32871472	6	27	theme	PD-L1	758:762	arg1	models					748:753	Two complementary tridimensional models	715:753	Two complementary tridimensional models	715:753	Two complementary tridimensional models of PD-L1, based on available crystallographic structures, were constructed with BMS-202 bound.					
32871472	9	28	theme	N-glycans	1052:1060	arg1	residues					1078:1085	8 N-glycans and 2 ubiquitin residues	1050:1085	8 N-glycans and 2 ubiquitin residues	1050:1085	Model 2 presents 8 N-glycans and 2 ubiquitin residues.					
32871472	14	29	gly	glycosylated	1672:1683	arg1	forms					1647:1651	the different forms	1633:1651	the different forms	1633:1651	Our modeling study suggests that the drug can target efficiently the different forms of PD-L1 in cells, glycosylated, ubiquitinated or not.					
32871472	11	30	theme	ubiquitins	1216:1225	arg1	incorporation					1178:1190	The incorporation	1174:1190	The incorporation of the N-glycans or the ubiquitins	1174:1225	The incorporation of the N-glycans or the ubiquitins did not significantly alter the drug-protein recognition.					
32871472	14	31	dep	forms	1647:1651	arg1	not					1703:1705	not	1703:1705	not	1703:1705	Our modeling study suggests that the drug can target efficiently the different forms of PD-L1 in cells, glycosylated, ubiquitinated or not.					
32871472	14	31	dep	forms	1647:1651	arg1	ubiquitinated					1686:1698	ubiquitinated	1686:1698	ubiquitinated	1686:1698	Our modeling study suggests that the drug can target efficiently the different forms of PD-L1 in cells, glycosylated, ubiquitinated or not.					
32871472	14	31	dep	forms	1647:1651	arg1	glycosylated					1672:1683	glycosylated	1672:1683	glycosylated	1672:1683	Our modeling study suggests that the drug can target efficiently the different forms of PD-L1 in cells, glycosylated, ubiquitinated or not.					
32871472	15	32	theme	other	1787:1791	arg1	complexes					1807:1815	other PD-L1 protein complexes	1787:1815	other PD-L1 protein complexes	1787:1815	These models of N-glycosylated and ubiquitinated PD-L1 will be useful to study other PD-L1 protein complexes.					
32871472	2	33	theme	solid	296:300	arg1	tumors					302:307	solid tumors	296:307	solid tumors	296:307	Monoclonal antibodies targeting PD-1 or PD-L1 are used to treat different types of solid tumors and lymphoma.					
32871472	14	34	theme	different	1637:1645	arg1	forms					1647:1651	the different forms	1633:1651	the different forms	1633:1651	Our modeling study suggests that the drug can target efficiently the different forms of PD-L1 in cells, glycosylated, ubiquitinated or not.					
32871472	6	35	theme	crystallographic	784:799	arg1	structures					801:810	available crystallographic structures	774:810	available crystallographic structures	774:810	Two complementary tridimensional models of PD-L1, based on available crystallographic structures, were constructed with BMS-202 bound.					
32871472	5	36	theme	N-glycosylation	571:585	arg1	effect					561:566	the effect	557:566	the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202	557:690	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	0	37	dep	BMS-202	79:85	arg1	study					109:113	A molecular modeling study	88:113	BMS-202: A molecular modeling study	79:113	N-glycosylation and ubiquitinylation of PD-L1 do not restrict interaction with BMS-202: A molecular modeling study.					
32871472	13	38	theme	drug-protein	1498:1509	arg1	complexes					1511:1519	the drug-protein complexes	1494:1519	the drug-protein complexes	1494:1519	Calculations of the binding energies indicated that the glycosylation slightly reduces the stability of the drug-protein complexes but does not prevent the drug binding process.					
32871472	5	39	from	N219	611:614	arg1	N-glycosylation					571:585	N-glycosylation	571:585	N-glycosylation (at N35, N192, N200 and N219)	571:615	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	39	from	N219	611:614	arg1	effect					561:566	the effect	557:566	the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202	557:690	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	39	from	N219	611:614	arg1	mono-ubiquitination					621:639	mono-ubiquitination	621:639	mono-ubiquitination (at K178)	621:649	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	40	theme	PD-L1	654:658	arg1	N-glycosylation					571:585	N-glycosylation	571:585	N-glycosylation (at N35, N192, N200 and N219)	571:615	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	40	theme	PD-L1	654:658	arg1	mono-ubiquitination					621:639	mono-ubiquitination	621:639	mono-ubiquitination (at K178)	621:649	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	6	41	theme	tridimensional	733:746	arg1	models					748:753	Two complementary tridimensional models	715:753	Two complementary tridimensional models	715:753	Two complementary tridimensional models of PD-L1, based on available crystallographic structures, were constructed with BMS-202 bound.					
32871472	6	42	theme	available	774:782	arg1	structures					801:810	available crystallographic structures	774:810	available crystallographic structures	774:810	Two complementary tridimensional models of PD-L1, based on available crystallographic structures, were constructed with BMS-202 bound.					
32871472	2	43	theme	different	277:285	arg1	types					287:291	different types	277:291	different types of solid tumors and lymphoma	277:320	Monoclonal antibodies targeting PD-1 or PD-L1 are used to treat different types of solid tumors and lymphoma.					
32871472	15	44	theme	PD-L1	1757:1761	arg1	models					1714:1719	These models	1708:1719	These models of N-glycosylated and ubiquitinated PD-L1	1708:1761	These models of N-glycosylated and ubiquitinated PD-L1 will be useful to study other PD-L1 protein complexes.					
32871472	1	45	theme	major	187:191	arg1	checkpoint					171:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	1	45	theme	major	187:191	arg1	target					193:198	a major target	185:198	a major target in oncology	185:210	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	5	46	from	N200	602:605	arg1	N-glycosylation					571:585	N-glycosylation	571:585	N-glycosylation (at N35, N192, N200 and N219)	571:615	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	46	from	N200	602:605	arg1	effect					561:566	the effect	557:566	the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202	557:690	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	46	from	N200	602:605	arg1	mono-ubiquitination					621:639	mono-ubiquitination	621:639	mono-ubiquitination (at K178)	621:649	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	0	47	gly	N-glycosylation	0:14	arg1	PD-L1					40:44	PD-L1	40:44	PD-L1	40:44	N-glycosylation and ubiquitinylation of PD-L1 do not restrict interaction with BMS-202: A molecular modeling study.					
32871472	0	48	theme	PD-L1	40:44	arg1	ubiquitinylation					20:35	ubiquitinylation	20:35	ubiquitinylation	20:35	N-glycosylation and ubiquitinylation of PD-L1 do not restrict interaction with BMS-202: A molecular modeling study.					
32871472	0	48	theme	PD-L1	40:44	arg1	N-glycosylation					0:14	N-glycosylation	0:14	N-glycosylation	0:14	N-glycosylation and ubiquitinylation of PD-L1 do not restrict interaction with BMS-202: A molecular modeling study.					
32871472	4	49	theme	protein	451:457	arg1	dimers					459:464	PD-L1 protein dimers	445:464	PD-L1 protein dimers	445:464	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	2	50	theme	Monoclonal	213:222	arg1	antibodies					224:233	Monoclonal antibodies	213:233	Monoclonal antibodies targeting PD-1 or PD-L1	213:257	Monoclonal antibodies targeting PD-1 or PD-L1 are used to treat different types of solid tumors and lymphoma.					
32871472	1	51	from	target	193:198	arg1	oncology					203:210	oncology	203:210	oncology	203:210	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	3	52	theme	small	337:341	arg1	molecules					343:351	PD-L1-binding small molecules	323:351	PD-L1-binding small molecules	323:351	PD-L1-binding small molecules are also actively searched.					
32871472	4	53	theme	experimental	510:521	arg1	models					523:528	experimental models	510:528	experimental models	510:528	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	14	54	from	forms	1647:1651	arg1	cells					1665:1669	cells	1665:1669	cells	1665:1669	Our modeling study suggests that the drug can target efficiently the different forms of PD-L1 in cells, glycosylated, ubiquitinated or not.					
32871472	4	55	theme	PD-L1	445:449	arg1	dimers					459:464	PD-L1 protein dimers	445:464	PD-L1 protein dimers	445:464	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	12	56	theme	dimer	1330:1334	arg1	interface					1289:1297	The interface	1285:1297	The interface of the drug-stabilized protein dimer	1285:1334	The interface of the drug-stabilized protein dimer is unaffected by the glycosylation or ubiquitination.					
32871472	7	57	gly	glycosylated	870:881	arg1	structures					854:863	The structures	850:863	The structures	850:863	The structures were glycosylated, with a fucosylated bi-antennary N-glycan and ubiquitinated.					
32871472	14	58	theme	PD-L1	1656:1660	arg1	forms					1647:1651	the different forms	1633:1651	the different forms	1633:1651	Our modeling study suggests that the drug can target efficiently the different forms of PD-L1 in cells, glycosylated, ubiquitinated or not.					
32871472	14	59	from	PD-L1	1656:1660	arg1	cells					1665:1669	cells	1665:1669	cells	1665:1669	Our modeling study suggests that the drug can target efficiently the different forms of PD-L1 in cells, glycosylated, ubiquitinated or not.					
32871472	4	60	theme	biphenyl	406:413	arg1	BMS-202					420:426	the biphenyl drug BMS-202	402:426	the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models	402:528	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	4	60	theme	biphenyl	406:413	arg1	compound					390:397	The lead compound	381:397	The lead compound	381:397	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	12	61	theme	protein	1322:1328	arg1	dimer					1330:1334	the drug-stabilized protein dimer	1302:1334	the drug-stabilized protein dimer	1302:1334	The interface of the drug-stabilized protein dimer is unaffected by the glycosylation or ubiquitination.					
32871472	9	62	theme	ubiquitin	1068:1076	arg1	residues					1078:1085	8 N-glycans and 2 ubiquitin residues	1050:1085	8 N-glycans and 2 ubiquitin residues	1050:1085	Model 2 presents 8 N-glycans and 2 ubiquitin residues.					
32871472	2	63	theme	lymphoma	313:320	arg1	types					287:291	different types	277:291	different types of solid tumors and lymphoma	277:320	Monoclonal antibodies targeting PD-1 or PD-L1 are used to treat different types of solid tumors and lymphoma.					
32871472	5	64	with	interaction	667:677	arg1	BMS-202					684:690	BMS-202	684:690	BMS-202	684:690	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	7	65	theme	bi-antennary	903:914	arg1	N-glycan					916:923	a fucosylated bi-antennary N-glycan	889:923	a fucosylated bi-antennary N-glycan	889:923	The structures were glycosylated, with a fucosylated bi-antennary N-glycan and ubiquitinated.					
32871472	13	66	theme	complexes	1511:1519	arg1	stability					1481:1489	the stability	1477:1489	the stability of the drug-protein complexes	1477:1519	Calculations of the binding energies indicated that the glycosylation slightly reduces the stability of the drug-protein complexes but does not prevent the drug binding process.					
32871472	1	67	theme	Programmed	120:129	arg1	checkpoint					171:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	1	67	theme	Programmed	120:129	arg1	target					193:198	a major target	185:198	a major target in oncology	185:210	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	15	68	theme	ubiquitinated	1743:1755	arg1	PD-L1					1757:1761	N-glycosylated and ubiquitinated PD-L1	1724:1761	N-glycosylated and ubiquitinated PD-L1	1724:1761	These models of N-glycosylated and ubiquitinated PD-L1 will be useful to study other PD-L1 protein complexes.					
32871472	11	69	theme	N-glycans	1199:1207	arg1	incorporation					1178:1190	The incorporation	1174:1190	The incorporation of the N-glycans or the ubiquitins	1174:1225	The incorporation of the N-glycans or the ubiquitins did not significantly alter the drug-protein recognition.					
32871472	15	70	gly	N-glycosylated	1724:1737	arg1	PD-L1					1757:1761	N-glycosylated and ubiquitinated PD-L1	1724:1761	N-glycosylated and ubiquitinated PD-L1	1724:1761	These models of N-glycosylated and ubiquitinated PD-L1 will be useful to study other PD-L1 protein complexes.					
32871472	6	71	theme	complementary	719:731	arg1	models					748:753	Two complementary tridimensional models	715:753	Two complementary tridimensional models	715:753	Two complementary tridimensional models of PD-L1, based on available crystallographic structures, were constructed with BMS-202 bound.					
32871472	12	72	dep	glycosylation	1357:1369	arg1	the					1353:1355	the	1353:1355	the	1353:1355	The interface of the drug-stabilized protein dimer is unaffected by the glycosylation or ubiquitination.					
32871472	1	73	theme	cell	131:134	arg1	checkpoint					171:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	1	73	theme	cell	131:134	arg1	target					193:198	a major target	185:198	a major target in oncology	185:210	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	5	74	from	effect	561:566	arg1	N200					602:605	N200	602:605	N200	602:605	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	74	from	effect	561:566	arg1	interaction					667:677	the interaction	663:677	the interaction with BMS-202	663:690	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	74	from	effect	561:566	arg1	N219					611:614	N219	611:614	N219	611:614	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	74	from	effect	561:566	arg1	K178					645:648	K178	645:648	K178	645:648	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	74	from	effect	561:566	arg1	N35					591:593	N35	591:593	N35	591:593	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	74	from	effect	561:566	arg1	N192					596:599	N192	596:599	N192	596:599	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	4	75	theme	drug	415:418	arg1	BMS-202					420:426	the biphenyl drug BMS-202	402:426	the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models	402:528	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	4	75	theme	drug	415:418	arg1	compound					390:397	The lead compound	381:397	The lead compound	381:397	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	1	76	theme	Death	136:140	arg1	checkpoint					171:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	1	76	theme	Death	136:140	arg1	target					193:198	a major target	185:198	a major target in oncology	185:210	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	15	77	theme	N-glycosylated	1724:1737	arg1	PD-L1					1757:1761	N-glycosylated and ubiquitinated PD-L1	1724:1761	N-glycosylated and ubiquitinated PD-L1	1724:1761	These models of N-glycosylated and ubiquitinated PD-L1 will be useful to study other PD-L1 protein complexes.					
32871472	10	78	theme	protein	1128:1134	arg1	interface					1136:1144	the protein interface	1124:1144	the protein interface	1124:1144	In both cases, BMS-202 was bound to the protein interface, stabilizing a PD-L1 dimer.					
32871472	15	79	theme	PD-L1	1793:1797	arg1	complexes					1807:1815	other PD-L1 protein complexes	1787:1815	other PD-L1 protein complexes	1787:1815	These models of N-glycosylated and ubiquitinated PD-L1 will be useful to study other PD-L1 protein complexes.					
32871472	4	80	theme	antitumor	488:496	arg1	activity					498:505	a potent antitumor activity	479:505	a potent antitumor activity in experimental models	479:528	The lead compound is the biphenyl drug BMS-202 which stabilizes PD-L1 protein dimers and displays a potent antitumor activity in experimental models.					
32871472	7	81	gly	fucosylated	891:901	arg1	N-glycan					916:923	a fucosylated bi-antennary N-glycan	889:923	a fucosylated bi-antennary N-glycan	889:923	The structures were glycosylated, with a fucosylated bi-antennary N-glycan and ubiquitinated.					
32871472	1	82	theme	protein-1/Ligand	142:157	arg1	checkpoint					171:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint	116:180	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	1	82	theme	protein-1/Ligand	142:157	arg1	target					193:198	a major target	185:198	a major target in oncology	185:210	The Programmed cell Death protein-1/Ligand 1 (PD-1/L1) checkpoint is a major target in oncology.					
32871472	5	83	from	K178	645:648	arg1	N-glycosylation					571:585	N-glycosylation	571:585	N-glycosylation (at N35, N192, N200 and N219)	571:615	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	83	from	K178	645:648	arg1	effect					561:566	the effect	557:566	the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202	557:690	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32871472	5	83	from	K178	645:648	arg1	mono-ubiquitination					621:639	mono-ubiquitination	621:639	mono-ubiquitination (at K178)	621:649	Here we have investigated the effect of N-glycosylation (at N35, N192, N200 and N219) and mono-ubiquitination (at K178) of PD-L1 on the interaction with BMS-202 by molecular modeling.					
32066783	4	0	theme	normal	692:697	arg1	plasma					707:712	normal seminal plasma	692:712	normal seminal plasma	692:712	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	9	1	theme	new	1435:1437	arg1	markers					1454:1460	new cancer-related markers	1435:1460	new cancer-related markers	1435:1460	These PSAs will be candidates for new cancer-related markers.					
32066783	7	2	dep	elucidated	1155:1164	arg1	other					1272:1276	other	1272:1276	other	1272:1276	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	7	2	dep	elucidated	1155:1164	arg1	one					1197:1199	one	1197:1199	one	1197:1199	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	7	3	dep	other	1272:1276	arg1	PSA					1304:1306	a low molecular weight PSA	1281:1306	a low molecular weight PSA without N-glycans	1281:1324	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	5	4	theme	agglutinin	880:889	arg1	LacdiNAc					908:915	LacdiNAc	908:915	LacdiNAc	908:915	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	5	4	theme	agglutinin	880:889	arg1	glycan					900:905	Wisteria floribunda agglutinin reactive glycan	860:905	Wisteria floribunda agglutinin reactive glycan (LacdiNAc)	860:916	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	5	5	theme	reactive	891:898	arg1	LacdiNAc					908:915	LacdiNAc	908:915	LacdiNAc	908:915	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	5	5	theme	reactive	891:898	arg1	glycan					900:905	Wisteria floribunda agglutinin reactive glycan	860:905	Wisteria floribunda agglutinin reactive glycan (LacdiNAc)	860:916	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	6	6	dep	-unbound	1018:1025	arg1	A					1009:1009	concanavalin A	996:1009	concanavalin A	996:1009	Conversely, concanavalin A (Con A)-unbound PSA was definitely detected from the three cancer origins but was almost negligible in seminal PSA.					
32066783	6	6	dep	-unbound	1018:1025	arg1	A					1016:1016	Con A	1012:1016	Con A	1012:1016	Conversely, concanavalin A (Con A)-unbound PSA was definitely detected from the three cancer origins but was almost negligible in seminal PSA.					
32066783	3	7	theme	cancer-related	580:593	arg1	glycoforms					595:604	cancer-related glycoforms	580:604	cancer-related glycoforms of PSA	580:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	7	8	dep	one	1197:1199	arg1	PSA					1228:1230	a high molecular weight PSA	1204:1230	a high molecular weight PSA with highly branched N-glycans	1204:1261	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	0	9	theme	new	107:109	arg1	candidates					111:120	new candidates	107:120	new candidates for prostate cancer detection	107:150	Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.					
32066783	3	10	contain	have	488:491	arg1	CTOS					476:479	CTOS	476:479	CTOS	476:479	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	3	10	contain	have	488:491	arg2	characteristics					501:515	similar characteristics	493:515	similar characteristics	493:515	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	3	10	contain	have	488:491	arg1	spheroids					465:473	cancer tissue-originated spheroids	440:473	cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA	440:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	9	11	theme	cancer-related	1439:1452	arg1	markers					1454:1460	new cancer-related markers	1435:1460	new cancer-related markers	1435:1460	These PSAs will be candidates for new cancer-related markers.					
32066783	7	12	theme	A-unbound	1177:1185	arg1	fraction					1187:1194	the Con A-unbound fraction	1169:1194	the Con A-unbound fraction	1169:1194	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	2	13	theme	glycans	308:314	arg1	structure					279:287	the structure	275:287	the structure of cancer-specific glycans	275:314	Understanding the structure of cancer-specific glycans can help us improve PSA assay.					
32066783	6	14	from	PSA	1122:1124	arg1	negligible					1100:1109	negligible	1100:1109	negligible	1100:1109	Conversely, concanavalin A (Con A)-unbound PSA was definitely detected from the three cancer origins but was almost negligible in seminal PSA.					
32066783	0	15	theme	prostate	126:133	arg1	detection					142:150	prostate cancer detection	126:150	prostate cancer detection	126:150	Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.					
32066783	5	16	theme	glycan	900:905	arg1	content					849:855	the content	845:855	the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc)	845:916	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	5	16	theme	glycan	900:905	arg1	similar					922:928	similar	922:928	similar	922:928	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	2	17	theme	cancer-specific	292:306	arg1	glycans					308:314	cancer-specific glycans	292:314	cancer-specific glycans	292:314	Understanding the structure of cancer-specific glycans can help us improve PSA assay.					
32066783	3	18	theme	cancer	440:445	arg1	CTOS					476:479	CTOS	476:479	CTOS	476:479	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	3	18	theme	cancer	440:445	arg1	spheroids					465:473	cancer tissue-originated spheroids	440:473	cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA	440:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	6	19	theme	cancer	1070:1075	arg1	origins					1077:1083	the three cancer origins	1060:1083	the three cancer origins	1060:1083	Conversely, concanavalin A (Con A)-unbound PSA was definitely detected from the three cancer origins but was almost negligible in seminal PSA.					
32066783	6	20	theme	seminal	1114:1120	arg1	PSA					1122:1124	seminal PSA	1114:1124	seminal PSA	1114:1124	Conversely, concanavalin A (Con A)-unbound PSA was definitely detected from the three cancer origins but was almost negligible in seminal PSA.					
32066783	3	21	theme	present	354:360	arg1	study					362:366	the present study	350:366	the present study	350:366	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	5	22	gly	sialylated	830:839	arg1	PSA					805:807	PSA	805:807	PSA from CTOS	805:817	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	4	23	from	profile	625:631	arg1	CTOS					645:648	CTOS	645:648	CTOS	645:648	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	2	24	theme	PSA	336:338	arg1	assay					340:344	PSA assay	336:344	PSA assay	336:344	Understanding the structure of cancer-specific glycans can help us improve PSA assay.					
32066783	3	25	theme	parent	532:537	arg1	tumour					539:544	the parent tumour to explore the new candidates for cancer-related glycoforms of PSA	528:611	the parent tumour to explore the new candidates for cancer-related glycoforms of PSA	528:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	6	26	theme	-unbound	1018:1025	arg1	PSA					1027:1029	concanavalin A (Con A)-unbound PSA	996:1029	concanavalin A (Con A)-unbound PSA	996:1029	Conversely, concanavalin A (Con A)-unbound PSA was definitely detected from the three cancer origins but was almost negligible in seminal PSA.					
32066783	4	27	from	lines	730:734	arg1	PSA					683:685	PSA	683:685	PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry	683:802	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	28	theme	cell	725:728	arg1	22Rv1					747:751	22Rv1	747:751	22Rv1	747:751	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	28	theme	cell	725:728	arg1	LNCaP					737:741	LNCaP	737:741	LNCaP	737:741	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	28	theme	cell	725:728	arg1	lines					730:734	cancer cell lines	718:734	cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry	718:802	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	29	from	CTOS	645:648	arg1	PSA					636:638	PSA	636:638	PSA from CTOS	636:648	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	29	from	CTOS	645:648	arg1	profile					625:631	The glycan profile	614:631	The glycan profile of PSA from CTOS	614:648	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	7	30	theme	branched	1244:1251	arg1	N-glycans					1253:1261	highly branched N-glycans	1237:1261	highly branched N-glycans	1237:1261	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	7	31	theme	PSA	1146:1148	arg1	types					1137:1141	Two novel types	1127:1141	Two novel types of PSA	1127:1148	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	1	32	theme	used	208:211	arg1	biomarker					213:221	the most frequently used biomarker	188:221	the most frequently used biomarker for the screening of prostate cancer	188:258	Prostate-specific antigen (PSA) is the most frequently used biomarker for the screening of prostate cancer.					
32066783	1	32	theme	used	208:211	arg1	antigen					171:177	Prostate-specific antigen	153:177	Prostate-specific antigen (PSA)	153:183	Prostate-specific antigen (PSA) is the most frequently used biomarker for the screening of prostate cancer.					
32066783	0	33	theme	glycosylation	9:21	arg1	Study					0:4	Study	0:4	Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids	0:97	Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.					
32066783	0	34	theme	cancer	135:140	arg1	detection					142:150	prostate cancer detection	126:150	prostate cancer detection	126:150	Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.					
32066783	8	35	theme	Lewis	1357:1361	arg1	existence					1344:1352	the existence	1340:1352	the existence of Lewis X antigen group on PSA	1340:1384	Furthermore, the existence of Lewis X antigen group on PSA was indicated.					
32066783	3	36	theme	new	561:563	arg1	candidates					565:574	the new candidates	557:574	the new candidates for cancer-related glycoforms of PSA	557:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	6	37	theme	Con	1012:1014	arg1	A					1016:1016	Con A	1012:1016	Con A	1012:1016	Conversely, concanavalin A (Con A)-unbound PSA was definitely detected from the three cancer origins but was almost negligible in seminal PSA.					
32066783	0	38	theme	antigen	44:50	arg1	glycosylation					9:21	glycosylation	9:21	glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids	9:97	Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.					
32066783	5	39	attach	derived	945:951	arg2	PSA					941:943	PSA	941:943	PSA derived from seminal plasma and 22Rv1	941:981	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	5	39	attach	derived	945:951	arg1	22Rv1					977:981	22Rv1	977:981	22Rv1	977:981	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	5	39	attach	derived	945:951	arg1	plasma					966:971	plasma	966:971	plasma	966:971	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	7	40	with	PSA	1228:1230	arg1	N-glycans					1253:1261	highly branched N-glycans	1237:1261	highly branched N-glycans	1237:1261	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	7	41	theme	molecular	1211:1219	arg1	weight					1221:1226	a high molecular weight	1204:1226	a high molecular weight PSA with highly branched N-glycans	1204:1261	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	7	42	theme	molecular	1287:1295	arg1	weight					1297:1302	a low molecular weight	1281:1302	a low molecular weight PSA without N-glycans	1281:1324	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	6	43	from	negligible	1100:1109	arg1	PSA					1122:1124	seminal PSA	1114:1124	seminal PSA	1114:1124	Conversely, concanavalin A (Con A)-unbound PSA was definitely detected from the three cancer origins but was almost negligible in seminal PSA.					
32066783	3	44	gly	glycoforms	595:604	arg1	PSA					609:611	PSA	609:611	PSA	609:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	0	45	theme	prostate-specific	26:42	arg1	antigen					44:50	prostate-specific antigen	26:50	prostate-specific antigen secreted by cancer tissue-originated spheroids	26:97	Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.					
32066783	3	46	theme	culture	414:420	arg1	medium					422:427	culture medium	414:427	culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA	414:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	8	47	theme	antigen	1365:1371	arg1	group					1373:1377	antigen group	1365:1377	antigen group	1365:1377	Furthermore, the existence of Lewis X antigen group on PSA was indicated.					
32066783	3	48	theme	PSA	609:611	arg1	glycoforms					595:604	cancer-related glycoforms	580:604	cancer-related glycoforms of PSA	580:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	7	49	theme	weight	1221:1226	arg1	PSA					1228:1230	a high molecular weight PSA	1204:1230	a high molecular weight PSA with highly branched N-glycans	1204:1261	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	3	50	theme	PSA	396:398	arg1	glycans					385:391	the glycans	381:391	the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA	381:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	8	51	from	existence	1344:1352	arg1	PSA					1382:1384	PSA	1382:1384	PSA	1382:1384	Furthermore, the existence of Lewis X antigen group on PSA was indicated.					
32066783	0	52	gly	glycosylation	9:21	arg1	antigen					44:50	prostate-specific antigen	26:50	prostate-specific antigen secreted by cancer tissue-originated spheroids	26:97	Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.					
32066783	3	53	theme	similar	493:499	arg1	characteristics					501:515	similar characteristics	493:515	similar characteristics	493:515	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	3	54	contain	containing	429:438	arg1	medium					422:427	culture medium	414:427	culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA	414:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	3	54	contain	containing	429:438	arg2	CTOS					476:479	CTOS	476:479	CTOS	476:479	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	3	54	contain	containing	429:438	arg2	spheroids					465:473	cancer tissue-originated spheroids	440:473	cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA	440:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	7	55	theme	low	1283:1285	arg1	weight					1297:1302	a low molecular weight	1281:1302	a low molecular weight PSA without N-glycans	1281:1324	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	5	56	from	CTOS	814:817	arg1	PSA					805:807	PSA	805:807	PSA from CTOS	805:817	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	4	57	theme	seminal	699:705	arg1	plasma					707:712	normal seminal plasma	692:712	normal seminal plasma	692:712	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	8	58	theme	group	1373:1377	arg1	existence					1344:1352	the existence	1340:1352	the existence of Lewis X antigen group on PSA	1340:1384	Furthermore, the existence of Lewis X antigen group on PSA was indicated.					
32066783	5	59	theme	seminal	958:964	arg1	plasma					966:971	plasma	966:971	plasma	966:971	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	7	60	theme	weight	1297:1302	arg1	PSA					1304:1306	a low molecular weight PSA	1281:1306	a low molecular weight PSA without N-glycans	1281:1324	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	4	61	theme	cancer	718:723	arg1	22Rv1					747:751	22Rv1	747:751	22Rv1	747:751	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	61	theme	cancer	718:723	arg1	LNCaP					737:741	LNCaP	737:741	LNCaP	737:741	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	61	theme	cancer	718:723	arg1	lines					730:734	cancer cell lines	718:734	cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry	718:802	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	6	62	theme	concanavalin	996:1007	arg1	A					1009:1009	concanavalin A	996:1009	concanavalin A	996:1009	Conversely, concanavalin A (Con A)-unbound PSA was definitely detected from the three cancer origins but was almost negligible in seminal PSA.					
32066783	7	63	theme	high	1206:1209	arg1	weight					1221:1226	a high molecular weight	1204:1226	a high molecular weight PSA with highly branched N-glycans	1204:1261	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	4	64	theme	lectin	760:765	arg1	chromatography					767:780	lectin chromatography	760:780	lectin chromatography	760:780	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	1	65	theme	Prostate-specific	153:169	arg1	biomarker					213:221	the most frequently used biomarker	188:221	the most frequently used biomarker for the screening of prostate cancer	188:258	Prostate-specific antigen (PSA) is the most frequently used biomarker for the screening of prostate cancer.					
32066783	1	65	theme	Prostate-specific	153:169	arg1	PSA					180:182	PSA	180:182	PSA	180:182	Prostate-specific antigen (PSA) is the most frequently used biomarker for the screening of prostate cancer.					
32066783	1	65	theme	Prostate-specific	153:169	arg1	antigen					171:177	Prostate-specific antigen	153:177	Prostate-specific antigen (PSA)	153:183	Prostate-specific antigen (PSA) is the most frequently used biomarker for the screening of prostate cancer.					
32066783	4	66	from	plasma	707:712	arg1	PSA					683:685	PSA	683:685	PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry	683:802	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	67	dep	lines	730:734	arg1	22Rv1					747:751	22Rv1	747:751	22Rv1	747:751	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	67	dep	lines	730:734	arg1	LNCaP					737:741	LNCaP	737:741	LNCaP	737:741	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	67	dep	lines	730:734	arg1	lines					730:734	cancer cell lines	718:734	cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry	718:802	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	0	68	theme	tissue-originated	71:87	arg1	spheroids					89:97	cancer tissue-originated spheroids	64:97	cancer tissue-originated spheroids	64:97	Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.					
32066783	4	69	theme	mass	786:789	arg1	spectrometry					791:802	mass spectrometry	786:802	mass spectrometry	786:802	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	4	70	theme	PSA	636:638	arg1	profile					625:631	The glycan profile	614:631	The glycan profile of PSA from CTOS	614:648	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	5	71	theme	Wisteria	860:867	arg1	LacdiNAc					908:915	LacdiNAc	908:915	LacdiNAc	908:915	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	5	71	theme	Wisteria	860:867	arg1	glycan					900:905	Wisteria floribunda agglutinin reactive glycan	860:905	Wisteria floribunda agglutinin reactive glycan (LacdiNAc)	860:916	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	3	72	theme	tissue-originated	447:463	arg1	CTOS					476:479	CTOS	476:479	CTOS	476:479	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	3	72	theme	tissue-originated	447:463	arg1	spheroids					465:473	cancer tissue-originated spheroids	440:473	cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA	440:611	In the present study, we analysed the glycans of PSA obtained from culture medium containing cancer tissue-originated spheroids (CTOS) which have similar characteristics as that of the parent tumour to explore the new candidates for cancer-related glycoforms of PSA.					
32066783	1	73	theme	prostate	244:251	arg1	cancer					253:258	prostate cancer	244:258	prostate cancer	244:258	Prostate-specific antigen (PSA) is the most frequently used biomarker for the screening of prostate cancer.					
32066783	0	74	theme	cancer	64:69	arg1	spheroids					89:97	cancer tissue-originated spheroids	64:97	cancer tissue-originated spheroids	64:97	Study of glycosylation of prostate-specific antigen secreted by cancer tissue-originated spheroids reveals new candidates for prostate cancer detection.					
32066783	7	75	theme	novel	1131:1135	arg1	types					1137:1141	Two novel types	1127:1141	Two novel types of PSA	1127:1148	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	4	76	theme	glycan	618:623	arg1	profile					625:631	The glycan profile	614:631	The glycan profile of PSA from CTOS	614:648	The glycan profile of PSA from CTOS was determined by comparing with PSA from normal seminal plasma and cancer cell lines (LNCaP and 22Rv1) using lectin chromatography and mass spectrometry.					
32066783	5	77	theme	floribunda	869:878	arg1	LacdiNAc					908:915	LacdiNAc	908:915	LacdiNAc	908:915	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	5	77	theme	floribunda	869:878	arg1	glycan					900:905	Wisteria floribunda agglutinin reactive glycan	860:905	Wisteria floribunda agglutinin reactive glycan (LacdiNAc)	860:916	PSA from CTOS was mostly sialylated and the content of Wisteria floribunda agglutinin reactive glycan (LacdiNAc) was similar to that of PSA derived from seminal plasma and 22Rv1.					
32066783	7	78	theme	Con	1173:1175	arg1	fraction					1187:1194	the Con A-unbound fraction	1169:1194	the Con A-unbound fraction	1169:1194	Two novel types of PSA were elucidated in the Con A-unbound fraction: one is a high molecular weight PSA with highly branched N-glycans, and the other is a low molecular weight PSA without N-glycans.					
32066783	1	79	theme	cancer	253:258	arg1	screening					231:239	the screening	227:239	the screening of prostate cancer	227:258	Prostate-specific antigen (PSA) is the most frequently used biomarker for the screening of prostate cancer.					
33908020	5	0	theme	consistent	624:633	arg1	results					635:641	consistent results	624:641	consistent results	624:641	Both approaches yield consistent results but it appears FTIR analysis is easier and more rapid to perform comparisons.					
33908020	4	1	theme	FTIR	554:557	arg1	information					559:569	This FTIR information	549:569	This FTIR information	549:569	This FTIR information is compared to MS information.					
33908020	3	2	theme	spectral	340:347	arg1	region					349:354	a spectral region	338:354	a spectral region specific to carbohydrate absorption	338:390	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	1	3	used	used	110:113	arg2	spectroscopy					81:92	FTIR spectroscopy	76:92	FTIR spectroscopy	76:92	FTIR spectroscopy has been widely used to characterize biopharmaceuticals for many years, in particular to analyze protein structure.					
33908020	1	4	theme	FTIR	76:79	arg1	spectroscopy					81:92	FTIR spectroscopy	76:92	FTIR spectroscopy	76:92	FTIR spectroscopy has been widely used to characterize biopharmaceuticals for many years, in particular to analyze protein structure.					
33908020	3	5	theme	detailed	411:418	arg1	protocol					420:427	a detailed protocol	409:427	a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition	409:546	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	3	6	from	spectra	449:455	arg1	terms					520:524	terms	520:524	terms of glycan composition	520:546	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	3	6	from	spectra	449:455	arg1	terms					477:481	terms	477:481	terms of global glycosylation level	477:511	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	3	7	theme	composition	536:546	arg1	terms					520:524	terms	520:524	terms of glycan composition	520:546	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	3	8	theme	specific	356:363	arg1	region					349:354	a spectral region	338:354	a spectral region specific to carbohydrate absorption	338:390	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	3	9	theme	carbohydrate	368:379	arg1	absorption					381:390	carbohydrate absorption	368:390	carbohydrate absorption	368:390	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	3	10	gly	glycoproteins	460:472	arg1	glycoproteins					460:472	glycoproteins	460:472	glycoproteins	460:472	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	1	11	theme	protein	191:197	arg1	structure					199:207	protein structure	191:207	protein structure	191:207	FTIR spectroscopy has been widely used to characterize biopharmaceuticals for many years, in particular to analyze protein structure.					
33908020	2	12	theme	protein	286:292	arg1	samples					294:300	protein samples	286:300	protein samples	286:300	More recently, it was demonstrated to be a useful tool to study and compare protein samples in terms of glycosylation.					
33908020	0	13	theme	Glycoproteins	12:24	arg1	Analysis					0:7	Analysis	0:7	Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment.	0:74	Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment.					
33908020	3	14	theme	level	507:511	arg1	terms					477:481	terms	477:481	terms of global glycosylation level	477:511	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	3	15	theme	glycan	529:534	arg1	composition					536:546	glycan composition	529:546	glycan composition	529:546	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	3	16	theme	FTIR	444:447	arg1	spectra					449:455	the FTIR spectra	440:455	the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition	440:546	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	0	17	theme	ATR-FTIR	29:36	arg1	Spectroscopy					38:49	ATR-FTIR Spectroscopy	29:49	ATR-FTIR Spectroscopy	29:49	Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment.					
33908020	0	18	theme	Comparative	52:62	arg1	Assessment					64:73	Comparative Assessment	52:73	Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment.	0:74	Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment.					
33908020	3	19	theme	glycoproteins	460:472	arg1	spectra					449:455	the FTIR spectra	440:455	the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition	440:546	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	5	20	theme	FTIR	658:661	arg1	analysis					663:670	FTIR analysis	658:670	FTIR analysis	658:670	Both approaches yield consistent results but it appears FTIR analysis is easier and more rapid to perform comparisons.					
33908020	4	21	dep	information	589:599	arg1	is					571:572	is	571:572	is compared to MS information	571:599	This FTIR information is compared to MS information.					
33908020	2	22	theme	glycosylation	314:326	arg1	terms					305:309	terms	305:309	terms of glycosylation	305:326	More recently, it was demonstrated to be a useful tool to study and compare protein samples in terms of glycosylation.					
33908020	1	23	theme	many	154:157	arg1	years					159:163	many years	154:163	many years	154:163	FTIR spectroscopy has been widely used to characterize biopharmaceuticals for many years, in particular to analyze protein structure.					
33908020	2	24	theme	useful	253:258	arg1	tool					260:263	a useful tool	251:263	a useful tool	251:263	More recently, it was demonstrated to be a useful tool to study and compare protein samples in terms of glycosylation.					
33908020	2	24	theme	useful	253:258	arg1	it					225:226	it	225:226	it	225:226	More recently, it was demonstrated to be a useful tool to study and compare protein samples in terms of glycosylation.					
33908020	3	25	theme	global	486:491	arg1	level					507:511	global glycosylation level	486:511	global glycosylation level	486:511	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	0	26	dep	Analysis	0:7	arg1	Assessment					64:73	Comparative Assessment	52:73	Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment.	0:74	Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment.					
33908020	3	27	theme	glycosylation	493:505	arg1	level					507:511	global glycosylation level	486:511	global glycosylation level	486:511	Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition.					
33908020	4	28	theme	MS	586:587	arg1	information					589:599	MS information	586:599	MS information	586:599	This FTIR information is compared to MS information.					
34178940	2	0	theme	therapeutic	392:402	arg1	antibodies					415:424	therapeutic monoclonal antibodies	392:424	therapeutic monoclonal antibodies	392:424	Altered glycosylation profiles are associated with cancer and inflammatory diseases, as well as impacting the efficacy of therapeutic monoclonal antibodies.					
34178940	9	1	theme	formalin-fixed	1721:1734	arg1	sections					1776:1783	formalin-fixed paraffin-embedded ovarian cancer tissue sections	1721:1783	formalin-fixed paraffin-embedded ovarian cancer tissue sections	1721:1783	A total of 120 N-glycan structural and compositional isomers were obtained from formalin-fixed paraffin-embedded ovarian cancer tissue sections.					
34178940	6	2	theme	positive	1292:1299	arg1	mode					1305:1308	positive ion mode	1292:1308	positive ion mode	1292:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	6	3	theme	mass	1249:1252	arg1	spectrometer					1254:1265	an Agilent 6550 Q-TOF mass spectrometer	1227:1265	an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1227:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	4	4	theme	chromatography-mass	687:705	arg1	LC-MS					721:725	LC-MS	721:725	LC-MS	721:725	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	4	4	theme	chromatography-mass	687:705	arg1	technique					632:640	A commonly used technique	616:640	A commonly used technique for quantitative N-glycan analysis	616:675	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	4	4	theme	chromatography-mass	687:705	arg1	spectrometry					707:718	liquid chromatography-mass spectrometry	680:718	liquid chromatography-mass spectrometry (LC-MS)	680:726	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	2	5	theme	inflammatory	332:343	arg1	diseases					345:352	inflammatory diseases	332:352	inflammatory diseases	332:352	Altered glycosylation profiles are associated with cancer and inflammatory diseases, as well as impacting the efficacy of therapeutic monoclonal antibodies.					
34178940	9	6	theme	ovarian	1754:1760	arg1	sections					1776:1783	formalin-fixed paraffin-embedded ovarian cancer tissue sections	1721:1783	formalin-fixed paraffin-embedded ovarian cancer tissue sections	1721:1783	A total of 120 N-glycan structural and compositional isomers were obtained from formalin-fixed paraffin-embedded ovarian cancer tissue sections.					
34178940	6	7	theme	6550	1238:1241	arg1	spectrometer					1254:1265	an Agilent 6550 Q-TOF mass spectrometer	1227:1265	an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1227:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	10	8	theme	late-stage	1827:1836	arg1	tissues					1886:1892	early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues	1816:1892	early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues	1816:1892	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	5	9	theme	higher	969:974	arg1	capability					986:995	its higher resolving capability	965:995	its higher resolving capability	965:995	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	6	10	with	in-house	1181:1188	arg1	PGC					1205:1207	Hypercarb PGC	1195:1207	Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1195:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	6	10	with	in-house	1181:1188	arg1	3 µm					1210:1213	3 µm	1210:1213	3 µm	1210:1213	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	3	11	link	N-linked	514:521	arg1	glycans					523:529	enzymatically released N-linked glycans	491:529	enzymatically released N-linked glycans	491:529	Consisting of oligosaccharides attached to asparagine residues, enzymatically released N-linked glycans are analytically challenging due to the diversity of isomeric structures that exist.					
34178940	8	12	attach	released	1578:1585	arg2	N-glycans					1568:1576	N-glycans	1568:1576	N-glycans released from complex samples	1568:1606	N-glycans released from complex samples were also examined by PGC LC-MS.					
34178940	8	12	attach	released	1578:1585	arg1	samples					1600:1606	complex samples	1592:1606	complex samples	1592:1606	N-glycans released from complex samples were also examined by PGC LC-MS.					
34178940	5	13	theme	stationary	818:827	arg1	phases					829:834	normal stationary phases	811:834	normal stationary phases	811:834	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	3	14	theme	structures	593:602	arg1	diversity					571:579	the diversity	567:579	the diversity of isomeric structures that exist	567:613	Consisting of oligosaccharides attached to asparagine residues, enzymatically released N-linked glycans are analytically challenging due to the diversity of isomeric structures that exist.					
34178940	7	15	theme	low	1473:1475	arg1	coefficients					1477:1488	low coefficients	1473:1488	low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%)	1473:1565	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	4	16	theme	glycan	744:749	arg1	separation					751:760	glycan separation	744:760	glycan separation	744:760	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	2	17	theme	Altered	270:276	arg1	profiles					292:299	Altered glycosylation profiles	270:299	Altered glycosylation profiles	270:299	Altered glycosylation profiles are associated with cancer and inflammatory diseases, as well as impacting the efficacy of therapeutic monoclonal antibodies.					
34178940	1	18	theme	protein	249:255	arg1	trafficking					257:267	protein trafficking	249:267	protein trafficking	249:267	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	7	19	theme	N-glycans	1454:1462	arg1	majority					1442:1449	the majority	1438:1449	the majority of N-glycans	1438:1462	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	12	20	theme	columns	2220:2226	arg1	utility					2189:2195	the utility	2185:2195	the utility of in-house packed PGC columns for the robust and reproducible LC-MS analysis of N-glycans	2185:2286	This study establishes the utility of in-house packed PGC columns for the robust and reproducible LC-MS analysis of N-glycans.					
34178940	11	21	from	cancer	2077:2082	arg1	predominant					2043:2053	predominant	2043:2053	predominant	2043:2053	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	11	21	from	cancer	2077:2082	arg1	α2,3-linkage					2026:2037	the α2,3-linkage	2022:2037	the α2,3-linkage	2022:2037	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	5	22	theme	reproducible	1044:1055	arg1	manner					1057:1062	a robust and reproducible manner	1031:1062	a robust and reproducible manner	1031:1062	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	10	23	theme	acid	1952:1955	arg1	linkage					1957:1963	the α2,3- and α2,6-sialic acid linkage	1926:1963	the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan	1926:2010	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	1	24	theme	post-translational	149:166	arg1	modification					168:179	a common post-translational modification	140:179	a common post-translational modification that modulates biological processes such as the immune response and protein trafficking	140:267	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	1	24	theme	post-translational	149:166	arg1	glycosylation					123:135	Protein glycosylation	115:135	Protein glycosylation	115:135	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	6	25	theme	15 cm	1119:1123	arg1	capillary					1138:1146	a 15 cm fused silica capillary	1117:1146	a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1117:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	10	26	dep	formalin-fixed	1838:1851	arg1	paraffin-embedded					1853:1869	paraffin-embedded	1853:1869	paraffin-embedded	1853:1869	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	0	27	theme	Chromatography-Mass	59:77	arg1	Analysis					92:99	Liquid Chromatography-Mass Spectrometry Analysis	52:99	Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans	52:112	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.					
34178940	11	28	from	predominant	2043:2053	arg1	cancer					2077:2082	late-stage ovarian cancer	2058:2082	late-stage ovarian cancer	2058:2082	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	12	29	theme	packed	2209:2214	arg1	columns					2220:2226	in-house packed PGC columns	2200:2226	in-house packed PGC columns	2200:2226	This study establishes the utility of in-house packed PGC columns for the robust and reproducible LC-MS analysis of N-glycans.					
34178940	1	30	theme	biological	196:205	arg1	response					236:243	the immune response	225:243	the immune response	225:243	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	1	30	theme	biological	196:205	arg1	processes					207:215	biological processes	196:215	biological processes such as the immune response and protein trafficking	196:267	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	1	30	theme	biological	196:205	arg1	trafficking					257:267	protein trafficking	249:267	protein trafficking	249:267	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	6	31	theme	analytical	1092:1101	arg1	properties					1103:1112	the analytical properties	1088:1112	the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1088:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	5	32	dep	reversed	798:805	arg1	many					793:796	many	793:796	many	793:796	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	6	33	dep	capillary	1138:1146	arg1	i.d.					1155:1158	75 µm i.d.	1149:1158	75 µm i.d.	1149:1158	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	6	33	dep	capillary	1138:1146	arg1	o.d.					1168:1171	360 µm o.d.	1161:1171	360 µm o.d.	1161:1171	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	0	34	theme	N-Glycans	104:112	arg1	Analysis					92:99	Liquid Chromatography-Mass Spectrometry Analysis	52:99	Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans	52:112	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.					
34178940	3	35	theme	N-linked	514:521	arg1	glycans					523:529	enzymatically released N-linked glycans	491:529	enzymatically released N-linked glycans	491:529	Consisting of oligosaccharides attached to asparagine residues, enzymatically released N-linked glycans are analytically challenging due to the diversity of isomeric structures that exist.					
34178940	6	36	theme	360 µm	1161:1166	arg1	i.d.					1155:1158	75 µm i.d.	1149:1158	75 µm i.d.	1149:1158	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	6	36	theme	360 µm	1161:1166	arg1	o.d.					1168:1171	360 µm o.d.	1161:1171	360 µm o.d.	1161:1171	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	8	37	theme	complex	1592:1598	arg1	samples					1600:1606	complex samples	1592:1606	complex samples	1592:1606	N-glycans released from complex samples were also examined by PGC LC-MS.					
34178940	0	38	theme	In-House	0:7	arg1	Columns					40:46	In-House Packed Porous Graphitic Carbon Columns	0:46	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.	0:113	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.					
34178940	7	39	theme	glycoprotein	1407:1418	arg1	mixture					1429:1435	a glycoprotein standard mixture	1405:1435	a glycoprotein standard mixture	1405:1435	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	11	40	theme	ovarian	2069:2075	arg1	cancer					2077:2082	late-stage ovarian cancer	2058:2082	late-stage ovarian cancer	2058:2082	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	0	41	theme	Porous	16:21	arg1	Columns					40:46	In-House Packed Porous Graphitic Carbon Columns	0:46	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.	0:113	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.					
34178940	11	42	theme	early-stage	2134:2144	arg1	cancer					2154:2159	early-stage ovarian cancer	2134:2159	early-stage ovarian cancer	2134:2159	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	4	43	theme	quantitative	646:657	arg1	analysis					668:675	quantitative N-glycan analysis	646:675	quantitative N-glycan analysis	646:675	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	10	44	theme	fucosylated	1970:1980	arg1	N-glycan					2003:2010	a fucosylated bi-antennary complex N-glycan	1968:2010	a fucosylated bi-antennary complex N-glycan	1968:2010	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	0	45	theme	Carbon	33:38	arg1	Columns					40:46	In-House Packed Porous Graphitic Carbon Columns	0:46	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.	0:113	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.					
34178940	5	46	theme	graphitic	895:903	arg1	chromatography					918:931	porous graphitic carbon (PGC) chromatography	888:931	porous graphitic carbon (PGC) chromatography	888:931	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	7	47	theme	released	1381:1388	arg1	N-glycans					1390:1398	released N-glycans	1381:1398	released N-glycans	1381:1398	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	8	48	theme	PGC	1630:1632	arg1	LC-MS					1634:1638	PGC LC-MS	1630:1638	PGC LC-MS	1630:1638	N-glycans released from complex samples were also examined by PGC LC-MS.					
34178940	9	49	theme	structural	1665:1674	arg1	isomers					1694:1700	120 N-glycan structural and compositional isomers	1652:1700	120 N-glycan structural and compositional isomers	1652:1700	A total of 120 N-glycan structural and compositional isomers were obtained from formalin-fixed paraffin-embedded ovarian cancer tissue sections.					
34178940	7	50	theme	retention	1519:1527	arg1	times					1529:1533	retention times	1519:1533	retention times (≤4.2%)	1519:1541	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	7	50	theme	retention	1519:1527	arg1	%					1540:1540	≤4.2%	1536:1540	≤4.2%	1536:1540	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	11	51	theme	alternate	2089:2097	arg1	α2,6-linkage					2099:2110	the alternate α2,6-linkage	2085:2110	the alternate α2,6-linkage	2085:2110	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	11	51	theme	alternate	2089:2097	arg1	prevalent					2121:2129	prevalent	2121:2129	prevalent	2121:2129	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	9	52	theme	compositional	1680:1692	arg1	isomers					1694:1700	120 N-glycan structural and compositional isomers	1652:1700	120 N-glycan structural and compositional isomers	1652:1700	A total of 120 N-glycan structural and compositional isomers were obtained from formalin-fixed paraffin-embedded ovarian cancer tissue sections.					
34178940	7	53	theme	precision	1345:1353	arg1	measurements					1355:1366	repeatability and intermediate precision measurements	1314:1366	repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture	1314:1435	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	6	54	theme	silica	1131:1136	arg1	capillary					1138:1146	a 15 cm fused silica capillary	1117:1146	a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1117:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	0	55	theme	Liquid	52:57	arg1	Analysis					92:99	Liquid Chromatography-Mass Spectrometry Analysis	52:99	Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans	52:112	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.					
34178940	2	56	theme	monoclonal	404:413	arg1	antibodies					415:424	therapeutic monoclonal antibodies	392:424	therapeutic monoclonal antibodies	392:424	Altered glycosylation profiles are associated with cancer and inflammatory diseases, as well as impacting the efficacy of therapeutic monoclonal antibodies.					
34178940	7	57	theme	peak	1547:1550	arg1	areas					1552:1556	peak areas	1547:1556	peak areas (≤14.4%)	1547:1565	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	7	57	theme	peak	1547:1550	arg1	%					1564:1564	≤14.4%	1559:1564	≤14.4%	1559:1564	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	9	58	theme	tissue	1769:1774	arg1	sections					1776:1783	formalin-fixed paraffin-embedded ovarian cancer tissue sections	1721:1783	formalin-fixed paraffin-embedded ovarian cancer tissue sections	1721:1783	A total of 120 N-glycan structural and compositional isomers were obtained from formalin-fixed paraffin-embedded ovarian cancer tissue sections.					
34178940	11	59	from	cancer	2154:2159	arg1	α2,6-linkage					2099:2110	the alternate α2,6-linkage	2085:2110	the alternate α2,6-linkage	2085:2110	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	11	59	from	cancer	2154:2159	arg1	prevalent					2121:2129	prevalent	2121:2129	prevalent	2121:2129	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	10	60	theme	ovarian	1871:1877	arg1	tissues					1886:1892	early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues	1816:1892	early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues	1816:1892	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	9	61	theme	paraffin-embedded	1736:1752	arg1	sections					1776:1783	formalin-fixed paraffin-embedded ovarian cancer tissue sections	1721:1783	formalin-fixed paraffin-embedded ovarian cancer tissue sections	1721:1783	A total of 120 N-glycan structural and compositional isomers were obtained from formalin-fixed paraffin-embedded ovarian cancer tissue sections.					
34178940	6	62	theme	Q-TOF	1243:1247	arg1	spectrometer					1254:1265	an Agilent 6550 Q-TOF mass spectrometer	1227:1265	an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1227:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	4	63	theme	liquid	680:685	arg1	LC-MS					721:725	LC-MS	721:725	LC-MS	721:725	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	4	63	theme	liquid	680:685	arg1	technique					632:640	A commonly used technique	616:640	A commonly used technique for quantitative N-glycan analysis	616:675	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	4	63	theme	liquid	680:685	arg1	spectrometry					707:718	liquid chromatography-mass spectrometry	680:718	liquid chromatography-mass spectrometry (LC-MS)	680:726	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	10	64	theme	formalin-fixed	1838:1851	arg1	tissues					1886:1892	early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues	1816:1892	early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues	1816:1892	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	9	65	theme	cancer	1762:1767	arg1	sections					1776:1783	formalin-fixed paraffin-embedded ovarian cancer tissue sections	1721:1783	formalin-fixed paraffin-embedded ovarian cancer tissue sections	1721:1783	A total of 120 N-glycan structural and compositional isomers were obtained from formalin-fixed paraffin-embedded ovarian cancer tissue sections.					
34178940	6	66	theme	Agilent	1230:1236	arg1	spectrometer					1254:1265	an Agilent 6550 Q-TOF mass spectrometer	1227:1265	an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1227:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	11	67	from	prevalent	2121:2129	arg1	cancer					2154:2159	early-stage ovarian cancer	2134:2159	early-stage ovarian cancer	2134:2159	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	10	68	theme	qualitative	1903:1913	arg1	changes					1915:1921	qualitative changes	1903:1921	qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan	1903:2010	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	5	69	theme	resolving	976:984	arg1	capability					986:995	its higher resolving capability	965:995	its higher resolving capability	965:995	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	12	70	theme	N-glycans	2278:2286	arg1	analysis					2266:2273	the robust and reproducible LC-MS analysis	2232:2273	the robust and reproducible LC-MS analysis of N-glycans	2232:2286	This study establishes the utility of in-house packed PGC columns for the robust and reproducible LC-MS analysis of N-glycans.					
34178940	5	71	theme	normal	811:816	arg1	phases					829:834	normal stationary phases	811:834	normal stationary phases	811:834	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	1	72	theme	immune	229:234	arg1	response					236:243	the immune response	225:243	the immune response	225:243	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	7	73	theme	variation	1493:1501	arg1	coefficients					1477:1488	low coefficients	1473:1488	low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%)	1473:1565	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	12	74	theme	robust	2236:2241	arg1	analysis					2266:2273	the robust and reproducible LC-MS analysis	2232:2273	the robust and reproducible LC-MS analysis of N-glycans	2232:2286	This study establishes the utility of in-house packed PGC columns for the robust and reproducible LC-MS analysis of N-glycans.					
34178940	3	75	theme	isomeric	584:591	arg1	structures					593:602	isomeric structures	584:602	isomeric structures that exist	584:613	Consisting of oligosaccharides attached to asparagine residues, enzymatically released N-linked glycans are analytically challenging due to the diversity of isomeric structures that exist.					
34178940	2	76	theme	glycosylation	278:290	arg1	profiles					292:299	Altered glycosylation profiles	270:299	Altered glycosylation profiles	270:299	Altered glycosylation profiles are associated with cancer and inflammatory diseases, as well as impacting the efficacy of therapeutic monoclonal antibodies.					
34178940	6	77	theme	ion	1301:1303	arg1	mode					1305:1308	positive ion mode	1292:1308	positive ion mode	1292:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	12	78	theme	reproducible	2247:2258	arg1	analysis					2266:2273	the robust and reproducible LC-MS analysis	2232:2273	the robust and reproducible LC-MS analysis of N-glycans	2232:2286	This study establishes the utility of in-house packed PGC columns for the robust and reproducible LC-MS analysis of N-glycans.					
34178940	11	79	attach	predominant	2043:2053	arg2	predominant					2043:2053	predominant	2043:2053	predominant	2043:2053	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	11	79	attach	predominant	2043:2053	arg2	α2,3-linkage					2026:2037	the α2,3-linkage	2022:2037	the α2,3-linkage	2022:2037	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	11	79	attach	predominant	2043:2053	arg1	cancer					2077:2082	late-stage ovarian cancer	2058:2082	late-stage ovarian cancer	2058:2082	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	10	80	theme	early-	1816:1821	arg1	tissues					1886:1892	early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues	1816:1892	early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues	1816:1892	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	3	81	attach	attached	458:465	arg1	residues					481:488	asparagine residues	470:488	asparagine residues	470:488	Consisting of oligosaccharides attached to asparagine residues, enzymatically released N-linked glycans are analytically challenging due to the diversity of isomeric structures that exist.					
34178940	3	81	attach	attached	458:465	arg2	oligosaccharides					441:456	oligosaccharides	441:456	oligosaccharides attached to asparagine residues	441:488	Consisting of oligosaccharides attached to asparagine residues, enzymatically released N-linked glycans are analytically challenging due to the diversity of isomeric structures that exist.					
34178940	12	82	theme	PGC	2216:2218	arg1	columns					2220:2226	in-house packed PGC columns	2200:2226	in-house packed PGC columns	2200:2226	This study establishes the utility of in-house packed PGC columns for the robust and reproducible LC-MS analysis of N-glycans.					
34178940	1	83	theme	common	142:147	arg1	modification					168:179	a common post-translational modification	140:179	a common post-translational modification that modulates biological processes such as the immune response and protein trafficking	140:267	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	1	83	theme	common	142:147	arg1	glycosylation					123:135	Protein glycosylation	115:135	Protein glycosylation	115:135	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	6	84	theme	fused	1125:1129	arg1	capillary					1138:1146	a 15 cm fused silica capillary	1117:1146	a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1117:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	6	85	theme	N-glycan	1271:1278	arg1	analysis					1280:1287	N-glycan analysis	1271:1287	N-glycan analysis in positive ion mode	1271:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	0	86	theme	Spectrometry	79:90	arg1	Analysis					92:99	Liquid Chromatography-Mass Spectrometry Analysis	52:99	Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans	52:112	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.					
34178940	10	87	theme	α2,6-sialic	1940:1950	arg1	linkage					1957:1963	the α2,3- and α2,6-sialic acid linkage	1926:1963	the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan	1926:2010	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	10	88	gly	fucosylated	1970:1980	arg1	N-glycan					2003:2010	a fucosylated bi-antennary complex N-glycan	1968:2010	a fucosylated bi-antennary complex N-glycan	1968:2010	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	5	89	theme	N-glycans	877:885	arg1	separation					863:872	the separation	859:872	the separation of N-glycans	859:885	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	12	90	theme	in-house	2200:2207	arg1	columns					2220:2226	in-house packed PGC columns	2200:2226	in-house packed PGC columns	2200:2226	This study establishes the utility of in-house packed PGC columns for the robust and reproducible LC-MS analysis of N-glycans.					
34178940	6	91	from	analysis	1280:1287	arg1	mode					1305:1308	positive ion mode	1292:1308	positive ion mode	1292:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	5	92	used	utilized	846:853	arg2	phases					829:834	normal stationary phases	811:834	normal stationary phases	811:834	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	5	92	used	utilized	846:853	arg2	reversed					798:805	reversed	798:805	reversed	798:805	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	10	93	theme	cancer	1879:1884	arg1	tissues					1886:1892	early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues	1816:1892	early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues	1816:1892	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	6	94	theme	Hypercarb	1195:1203	arg1	PGC					1205:1207	Hypercarb PGC	1195:1207	Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1195:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	6	94	theme	Hypercarb	1195:1203	arg1	3 µm					1210:1213	3 µm	1210:1213	3 µm	1210:1213	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	10	95	from	changes	1915:1921	arg1	linkage					1957:1963	the α2,3- and α2,6-sialic acid linkage	1926:1963	the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan	1926:2010	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	7	96	theme	standard	1420:1427	arg1	mixture					1429:1435	a glycoprotein standard mixture	1405:1435	a glycoprotein standard mixture	1405:1435	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	5	97	theme	PGC	913:915	arg1	chromatography					918:931	porous graphitic carbon (PGC) chromatography	888:931	porous graphitic carbon (PGC) chromatography	888:931	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	7	98	gly	glycoprotein	1407:1418	arg1	glycoprotein					1407:1418	a glycoprotein standard mixture	1405:1435	a glycoprotein standard mixture	1405:1435	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	5	99	theme	robust	1033:1038	arg1	manner					1057:1062	a robust and reproducible manner	1031:1062	a robust and reproducible manner	1031:1062	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	10	100	theme	complex	1995:2001	arg1	N-glycan					2003:2010	a fucosylated bi-antennary complex N-glycan	1968:2010	a fucosylated bi-antennary complex N-glycan	1968:2010	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	3	101	theme	released	505:512	arg1	glycans					523:529	enzymatically released N-linked glycans	491:529	enzymatically released N-linked glycans	491:529	Consisting of oligosaccharides attached to asparagine residues, enzymatically released N-linked glycans are analytically challenging due to the diversity of isomeric structures that exist.					
34178940	0	102	theme	Graphitic	23:31	arg1	Columns					40:46	In-House Packed Porous Graphitic Carbon Columns	0:46	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.	0:113	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.					
34178940	1	103	theme	Protein	115:121	arg1	modification					168:179	a common post-translational modification	140:179	a common post-translational modification that modulates biological processes such as the immune response and protein trafficking	140:267	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	1	103	theme	Protein	115:121	arg1	glycosylation					123:135	Protein glycosylation	115:135	Protein glycosylation	115:135	Protein glycosylation is a common post-translational modification that modulates biological processes such as the immune response and protein trafficking.					
34178940	10	104	theme	α2,3-	1930:1934	arg1	linkage					1957:1963	the α2,3- and α2,6-sialic acid linkage	1926:1963	the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan	1926:2010	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	4	105	theme	N-glycan	659:666	arg1	analysis					668:675	quantitative N-glycan analysis	646:675	quantitative N-glycan analysis	646:675	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	10	106	theme	bi-antennary	1982:1993	arg1	N-glycan					2003:2010	a fucosylated bi-antennary complex N-glycan	1968:2010	a fucosylated bi-antennary complex N-glycan	1968:2010	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	5	107	theme	porous	888:893	arg1	chromatography					918:931	porous graphitic carbon (PGC) chromatography	888:931	porous graphitic carbon (PGC) chromatography	888:931	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	12	108	theme	LC-MS	2260:2264	arg1	analysis					2266:2273	the robust and reproducible LC-MS analysis	2232:2273	the robust and reproducible LC-MS analysis of N-glycans	2232:2286	This study establishes the utility of in-house packed PGC columns for the robust and reproducible LC-MS analysis of N-glycans.					
34178940	6	109	theme	capillary	1138:1146	arg1	properties					1103:1112	the analytical properties	1088:1112	the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode	1088:1308	Herein, we demonstrate the analytical properties of a 15 cm fused silica capillary (75 µm i.d., 360 µm o.d.) packed in-house with Hypercarb PGC (3 µm) coupled to an Agilent 6550 Q-TOF mass spectrometer for N-glycan analysis in positive ion mode.					
34178940	11	110	theme	late-stage	2058:2067	arg1	cancer					2077:2082	late-stage ovarian cancer	2058:2082	late-stage ovarian cancer	2058:2082	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	5	111	theme	carbon	905:910	arg1	chromatography					918:931	porous graphitic carbon (PGC) chromatography	888:931	porous graphitic carbon (PGC) chromatography	888:931	Although many reversed and normal stationary phases have been utilized for the separation of N-glycans, porous graphitic carbon (PGC) chromatography has become desirable because of its higher resolving capability, but is difficult to implement in a robust and reproducible manner.					
34178940	9	112	theme	120 N-glycan	1652:1663	arg1	isomers					1694:1700	120 N-glycan structural and compositional isomers	1652:1700	120 N-glycan structural and compositional isomers	1652:1700	A total of 120 N-glycan structural and compositional isomers were obtained from formalin-fixed paraffin-embedded ovarian cancer tissue sections.					
34178940	3	113	theme	asparagine	470:479	arg1	residues					481:488	asparagine residues	470:488	asparagine residues	470:488	Consisting of oligosaccharides attached to asparagine residues, enzymatically released N-linked glycans are analytically challenging due to the diversity of isomeric structures that exist.					
34178940	4	114	theme	used	627:630	arg1	spectrometry					707:718	liquid chromatography-mass spectrometry	680:718	liquid chromatography-mass spectrometry (LC-MS)	680:726	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	4	114	theme	used	627:630	arg1	technique					632:640	A commonly used technique	616:640	A commonly used technique for quantitative N-glycan analysis	616:675	A commonly used technique for quantitative N-glycan analysis is liquid chromatography-mass spectrometry (LC-MS), which performs glycan separation and characterization.					
34178940	7	115	theme	intermediate	1332:1343	arg1	measurements					1355:1366	repeatability and intermediate precision measurements	1314:1366	repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture	1314:1435	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	11	116	theme	ovarian	2146:2152	arg1	cancer					2154:2159	early-stage ovarian cancer	2134:2159	early-stage ovarian cancer	2134:2159	Although the α2,3-linkage was predominant in late-stage ovarian cancer, the alternate α2,6-linkage was more prevalent in early-stage ovarian cancer.					
34178940	10	117	theme	N-glycan	2003:2010	arg1	linkage					1957:1963	the α2,3- and α2,6-sialic acid linkage	1926:1963	the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan	1926:2010	Finally, a comparison between early- and late-stage formalin-fixed paraffin-embedded ovarian cancer tissues revealed qualitative changes in the α2,3- and α2,6-sialic acid linkage of a fucosylated bi-antennary complex N-glycan.					
34178940	2	118	theme	antibodies	415:424	arg1	efficacy					380:387	the efficacy	376:387	the efficacy of therapeutic monoclonal antibodies	376:424	Altered glycosylation profiles are associated with cancer and inflammatory diseases, as well as impacting the efficacy of therapeutic monoclonal antibodies.					
34178940	9	119	theme	isomers	1694:1700	arg1	total					1643:1647	A total	1641:1647	A total of 120 N-glycan structural and compositional isomers	1641:1700	A total of 120 N-glycan structural and compositional isomers were obtained from formalin-fixed paraffin-embedded ovarian cancer tissue sections.					
34178940	7	120	theme	repeatability	1314:1326	arg1	measurements					1355:1366	repeatability and intermediate precision measurements	1314:1366	repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture	1314:1435	In repeatability and intermediate precision measurements conducted on released N-glycans from a glycoprotein standard mixture, the majority of N-glycans reported low coefficients of variation with respect to retention times (≤4.2%) and peak areas (≤14.4%).					
34178940	0	121	theme	Packed	9:14	arg1	Columns					40:46	In-House Packed Porous Graphitic Carbon Columns	0:46	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.	0:113	In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of N-Glycans.					
34328324	2	0	theme	bottom-up	610:618	arg1	approaches					635:644	conventional bottom-up glycoproteomic approaches	597:644	conventional bottom-up glycoproteomic approaches	597:644	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	3	1	theme	intact	702:707	arg1	proteoforms					718:728	intact O-glycan proteoforms	702:728	intact O-glycan proteoforms	702:728	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	1	2	theme	host	268:271	arg1	entry					278:282	host cell entry	268:282	host cell entry	268:282	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	3	3	theme	gas-phase	1060:1068	arg1	heterogeneity					1081:1093	their gas-phase structural heterogeneity	1054:1093	their gas-phase structural heterogeneity	1054:1093	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	4	theme	native	747:752	arg1	time-of-flight					877:890	a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier	738:923	time-of-flight	877:890	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	5	5	theme	molecular	1543:1551	arg1	foundation					1553:1562	a strong molecular foundation	1534:1562	a strong molecular foundation to uncover the functional roles of their O-glycans	1534:1613	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	2	6	located	found	469:473	arg1	S-RBD					517:521	S-RBD	517:521	S-RBD	517:521	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	2	6	located	found	469:473	arg1	domain					509:514	the S protein regional-binding domain	478:514	the S protein regional-binding domain (S-RBD)	478:522	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	2	6	located	found	469:473	arg2	O-glycans					459:467	the new O-glycans	451:467	the new O-glycans found on the S protein regional-binding domain (S-RBD)	451:522	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	1	7	gly	glycosylated	218:229	arg1	protein					249:255	an extensively glycosylated surface spike (S) protein	203:255	an extensively glycosylated surface spike (S) protein	203:255	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	5	8	theme	glycoprotein	1509:1520	arg1	mapping					1468:1474	a high-resolution proteoform-resolved mapping	1430:1474	a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans	1430:1613	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	3	9	theme	mass	778:781	arg1	approach					801:808	denaturing top-down mass spectrometry (MS) approach	758:808	denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry	758:857	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	6	10	theme	other	1765:1769	arg1	O-glycoproteins					1771:1785	other O-glycoproteins	1765:1785	emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins	1719:1785	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	3	11	theme	denaturing	758:767	arg1	approach					801:808	denaturing top-down mass spectrometry (MS) approach	758:808	denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry	758:857	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	1	12	theme	surface	231:237	arg1	protein					249:255	an extensively glycosylated surface spike (S) protein	203:255	an extensively glycosylated surface spike (S) protein	203:255	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	6	13	from	heterogeneity	1702:1714	arg1	general					1790:1796	general	1790:1796	general	1790:1796	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	6	14	theme	SARS-CoV-2	1728:1737	arg1	variants					1745:1752	emergent SARS-CoV-2 S-RBD variants	1719:1752	emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins	1719:1785	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	2	15	theme	glycoform	575:583	arg1	analysis					585:592	intact glycoform analysis	568:592	intact glycoform analysis by conventional bottom-up glycoproteomic approaches	568:644	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	1	16	theme	acute	140:144	arg1	coronavirus					167:177	Severe acute respiratory syndrome coronavirus 2	133:179	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	133:192	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	1	16	theme	acute	140:144	arg1	SARS-CoV-2					182:191	SARS-CoV-2	182:191	SARS-CoV-2	182:191	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	3	17	theme	trapped	825:831	arg1	spectrometry					846:857	both trapped ion mobility spectrometry	820:857	both trapped ion mobility spectrometry	820:857	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	6	18	theme	O-glycoform	1690:1700	arg1	heterogeneity					1702:1714	the structural O-glycoform heterogeneity	1675:1714	the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general	1675:1796	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	1	19	theme	syndrome	158:165	arg1	coronavirus					167:177	Severe acute respiratory syndrome coronavirus 2	133:179	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	133:192	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	1	19	theme	syndrome	158:165	arg1	SARS-CoV-2					182:191	SARS-CoV-2	182:191	SARS-CoV-2	182:191	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	3	20	gly	glycosites	1224:1233	arg2	glycosites					1224:1233	their glycosites	1218:1233	their glycosites	1218:1233	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	1	21	theme	cell	273:276	arg1	entry					278:282	host cell entry	268:282	host cell entry	268:282	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	2	22	theme	protein	484:490	arg1	S-RBD					517:521	S-RBD	517:521	S-RBD	517:521	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	2	22	theme	protein	484:490	arg1	domain					509:514	the S protein regional-binding domain	478:514	the S protein regional-binding domain (S-RBD)	478:522	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	3	23	theme	in-depth	1130:1137	arg1	analysis					1149:1156	in-depth glycoform analysis	1130:1156	in-depth glycoform analysis	1130:1156	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	24	theme	ultrahigh-resolution	896:915	arg1	Fourier					917:923	a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier	738:923	Fourier	917:923	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	25	theme	mobility	837:844	arg1	spectrometry					846:857	both trapped ion mobility spectrometry	820:857	both trapped ion mobility spectrometry	820:857	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	0	26	theme	Top-Down	105:112	arg1	Spectrometry					119:130	Top-Down Mass Spectrometry	105:130	Top-Down Mass Spectrometry	105:130	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry.					
34328324	1	27	theme	viral	349:353	arg1	binding/function					355:370	the viral binding/function	345:370	the viral binding/function	345:370	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	3	28	dep	time-of-flight	877:890	arg1	transform					925:933	transform	925:933	transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	925:996	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	29	theme	unambiguous	1162:1172	arg1	identification					1174:1187	unambiguous identification	1162:1187	unambiguous identification of the glycan structures and their glycosites	1162:1233	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	30	theme	cyclotron	939:947	arg1	FTICR					960:964	FTICR	960:964	FTICR	960:964	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	30	theme	cyclotron	939:947	arg1	resonance					949:957	ion cyclotron resonance	935:957	ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	935:996	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	2	31	theme	new	455:457	arg1	O-glycans					459:467	the new O-glycans	451:467	the new O-glycans found on the S protein regional-binding domain (S-RBD)	451:522	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	4	32	theme	relative	1276:1283	arg1	abundance					1295:1303	their relative molecular abundance	1270:1303	their relative molecular abundance	1270:1303	A total of eight O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
34328324	5	33	theme	high-resolution	1432:1446	arg1	mapping					1468:1474	a high-resolution proteoform-resolved mapping	1430:1474	a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans	1430:1613	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	0	34	theme	Structural	0:9	arg1	Heterogeneity					23:35	Structural O-Glycoform Heterogeneity	0:35	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain	0:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry.					
34328324	6	35	theme	variants	1745:1752	arg1	heterogeneity					1702:1714	the structural O-glycoform heterogeneity	1675:1714	the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general	1675:1796	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	3	36	theme	Native	971:976	arg1	TIMS-MS/MS					987:996	ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	935:996	ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	935:996	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	5	37	theme	top-down	1397:1404	arg1	approach					1409:1416	this hybrid top-down MS approach	1385:1416	this hybrid top-down MS approach	1385:1416	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	2	38	theme	glycan	427:432	arg1	heterogeneity					434:446	glycan heterogeneity	427:446	glycan heterogeneity	427:446	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	3	39	theme	glycan	1196:1201	arg1	structures					1203:1212	the glycan structures	1192:1212	the glycan structures	1192:1212	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	2	40	dep	structures	412:421	arg1	the					398:400	the	398:400	the	398:400	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	1	41	theme	protein	295:301	arg1	glycosylation					303:315	the S protein glycosylation	289:315	the S protein glycosylation	289:315	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	6	42	gly	O-glycoproteins	1771:1785	arg1	O-glycoproteins					1771:1785	other O-glycoproteins	1765:1785	emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins	1719:1785	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	0	43	theme	Receptor-Binding	69:84	arg1	Domain					86:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry.					
34328324	0	44	theme	Spike	55:59	arg1	Domain					86:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry.					
34328324	3	45	theme	glycosites	1224:1233	arg1	identification					1174:1187	unambiguous identification	1162:1187	unambiguous identification of the glycan structures and their glycosites	1162:1233	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	4	46	theme	first	1341:1345	arg1	time					1347:1350	the first time	1337:1350	the first time	1337:1350	A total of eight O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
34328324	5	47	theme	S	1507:1507	arg1	glycoprotein					1509:1520	the S glycoprotein	1503:1520	the S glycoprotein	1503:1520	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	1	48	theme	S	246:246	arg1	protein					249:255	an extensively glycosylated surface spike (S) protein	203:255	an extensively glycosylated surface spike (S) protein	203:255	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	3	49	theme	structural	676:685	arg1	elucidation					687:697	the complete structural elucidation	663:697	the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites	663:1233	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	5	50	theme	diverse	1479:1485	arg1	O-glycoforms					1487:1498	diverse O-glycoforms	1479:1498	diverse O-glycoforms	1479:1498	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	2	51	theme	conventional	597:608	arg1	approaches					635:644	conventional bottom-up glycoproteomic approaches	597:644	conventional bottom-up glycoproteomic approaches	597:644	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	3	52	theme	O-glycan	709:716	arg1	proteoforms					718:728	intact O-glycan proteoforms	702:728	intact O-glycan proteoforms	702:728	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	5	53	theme	strong	1536:1541	arg1	foundation					1553:1562	a strong molecular foundation	1534:1562	a strong molecular foundation to uncover the functional roles of their O-glycans	1534:1613	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	3	54	dep	elucidation	687:697	arg1	provides					1121:1128	provides	1121:1128	provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites	1121:1233	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	54	dep	elucidation	687:697	arg1	separates					998:1006	separates	998:1006	separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity	998:1093	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	2	55	theme	glycoproteomic	620:633	arg1	approaches					635:644	conventional bottom-up glycoproteomic approaches	597:644	conventional bottom-up glycoproteomic approaches	597:644	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	3	56	theme	structural	1070:1079	arg1	heterogeneity					1081:1093	their gas-phase structural heterogeneity	1054:1093	their gas-phase structural heterogeneity	1054:1093	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	57	theme	spectrometry	783:794	arg1	approach					801:808	denaturing top-down mass spectrometry (MS) approach	758:808	denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry	758:857	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	6	58	theme	proteoform-resolved	1621:1639	arg1	approach					1641:1648	This proteoform-resolved approach	1616:1648	This proteoform-resolved approach	1616:1648	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	1	59	theme	glycosylated	218:229	arg1	protein					249:255	an extensively glycosylated surface spike (S) protein	203:255	an extensively glycosylated surface spike (S) protein	203:255	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	3	60	theme	top-down	769:776	arg1	approach					801:808	denaturing top-down mass spectrometry (MS) approach	758:808	denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry	758:857	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	61	dep	S-RBD	1038:1042	arg1	reveal					1047:1052	reveal	1047:1052	to reveal their gas-phase structural heterogeneity	1044:1093	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	1	62	theme	spike	239:243	arg1	protein					249:255	an extensively glycosylated surface spike (S) protein	203:255	an extensively glycosylated surface spike (S) protein	203:255	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	4	63	theme	O-glycoforms	1253:1264	arg1	total					1238:1242	A total	1236:1242	A total of eight O-glycoforms	1236:1264	A total of eight O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
34328324	4	63	theme	O-glycoforms	1253:1264	arg1	abundance					1295:1303	their relative molecular abundance	1270:1303	their relative molecular abundance	1270:1303	A total of eight O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
34328324	6	64	theme	emergent	1719:1726	arg1	variants					1745:1752	emergent SARS-CoV-2 S-RBD variants	1719:1752	emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins	1719:1785	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	1	65	theme	Severe	133:138	arg1	coronavirus					167:177	Severe acute respiratory syndrome coronavirus 2	133:179	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	133:192	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	1	65	theme	Severe	133:138	arg1	SARS-CoV-2					182:191	SARS-CoV-2	182:191	SARS-CoV-2	182:191	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	2	66	theme	intact	568:573	arg1	analysis					585:592	intact glycoform analysis	568:592	intact glycoform analysis by conventional bottom-up glycoproteomic approaches	568:644	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	3	67	theme	MS	797:798	arg1	approach					801:808	denaturing top-down mass spectrometry (MS) approach	758:808	denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry	758:857	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	6	68	theme	O-glycoproteins	1771:1785	arg1	heterogeneity					1702:1714	the structural O-glycoform heterogeneity	1675:1714	the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general	1675:1796	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	1	69	theme	respiratory	146:156	arg1	coronavirus					167:177	Severe acute respiratory syndrome coronavirus 2	133:179	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	133:192	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	1	69	theme	respiratory	146:156	arg1	SARS-CoV-2					182:191	SARS-CoV-2	182:191	SARS-CoV-2	182:191	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	3	70	theme	ion	833:835	arg1	spectrometry					846:857	both trapped ion mobility spectrometry	820:857	both trapped ion mobility spectrometry	820:857	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	0	71	theme	Domain	86:91	arg1	Heterogeneity					23:35	Structural O-Glycoform Heterogeneity	0:35	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain	0:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry.					
34328324	6	72	theme	structural	1679:1688	arg1	heterogeneity					1702:1714	the structural O-glycoform heterogeneity	1675:1714	the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general	1675:1796	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	3	73	theme	quadrupole	866:875	arg1	time-of-flight					877:890	a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier	738:923	time-of-flight	877:890	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	2	74	theme	regional-binding	492:507	arg1	S-RBD					517:521	S-RBD	517:521	S-RBD	517:521	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	2	74	theme	regional-binding	492:507	arg1	domain					509:514	the S protein regional-binding domain	478:514	the S protein regional-binding domain (S-RBD)	478:522	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	3	75	theme	ion	935:937	arg1	FTICR					960:964	FTICR	960:964	FTICR	960:964	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	75	theme	ion	935:937	arg1	resonance					949:957	ion cyclotron resonance	935:957	ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	935:996	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	2	76	theme	S	482:482	arg1	S-RBD					517:521	S-RBD	517:521	S-RBD	517:521	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	2	76	theme	S	482:482	arg1	domain					509:514	the S protein regional-binding domain	478:514	the S protein regional-binding domain (S-RBD)	478:522	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	5	77	gly	glycoprotein	1509:1520	arg1	glycoprotein					1509:1520	the S glycoprotein	1503:1520	the S glycoprotein	1503:1520	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	0	78	theme	Mass	114:117	arg1	Spectrometry					119:130	Top-Down Mass Spectrometry	105:130	Top-Down Mass Spectrometry	105:130	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry.					
34328324	3	79	theme	top-down	1100:1107	arg1	FTICR-MS/MS					1109:1119	top-down FTICR-MS/MS	1100:1119	top-down FTICR-MS/MS	1100:1119	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	80	dep	quadrupole	866:875	arg1	approach					801:808	denaturing top-down mass spectrometry (MS) approach	758:808	denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry	758:857	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	5	81	theme	O-glycans	1605:1613	arg1	roles					1590:1594	the functional roles	1575:1594	the functional roles of their O-glycans	1575:1613	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	4	82	theme	molecular	1285:1293	arg1	abundance					1295:1303	their relative molecular abundance	1270:1303	their relative molecular abundance	1270:1303	A total of eight O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
34328324	0	83	theme	O-Glycoform	11:21	arg1	Heterogeneity					23:35	Structural O-Glycoform Heterogeneity	0:35	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain	0:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry.					
34328324	3	84	theme	glycoform	1139:1147	arg1	analysis					1149:1156	in-depth glycoform analysis	1130:1156	in-depth glycoform analysis	1130:1156	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	85	theme	resonance	949:957	arg1	TIMS-MS/MS					987:996	ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	935:996	ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	935:996	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	5	86	theme	functional	1579:1588	arg1	roles					1590:1594	the functional roles	1575:1594	the functional roles of their O-glycans	1575:1613	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	5	87	theme	hybrid	1390:1395	arg1	approach					1409:1416	this hybrid top-down MS approach	1385:1416	this hybrid top-down MS approach	1385:1416	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	6	88	theme	S-RBD	1739:1743	arg1	variants					1745:1752	emergent SARS-CoV-2 S-RBD variants	1719:1752	emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins	1719:1785	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	3	89	theme	top-down	978:985	arg1	TIMS-MS/MS					987:996	ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	935:996	ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	935:996	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	5	90	theme	MS	1406:1407	arg1	approach					1409:1416	this hybrid top-down MS approach	1385:1416	this hybrid top-down MS approach	1385:1416	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	0	91	theme	SARS-CoV-2	44:53	arg1	Domain					86:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry.					
34328324	1	92	theme	S	293:293	arg1	glycosylation					303:315	the S protein glycosylation	289:315	the S protein glycosylation	289:315	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	3	93	theme	-MS.	966:969	arg1	TIMS-MS/MS					987:996	ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	935:996	ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS	935:996	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	5	94	theme	O-glycoforms	1487:1498	arg1	mapping					1468:1474	a high-resolution proteoform-resolved mapping	1430:1474	a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans	1430:1613	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	2	95	theme	molecular	402:410	arg1	structures					412:421	molecular structures	402:421	molecular structures	402:421	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	0	96	theme	Protein	61:67	arg1	Domain					86:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry.					
34328324	0	97	gly	Heterogeneity	23:35	arg1	Domain					86:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry.					
34328324	3	98	theme	protein	1012:1018	arg1	conformers					1020:1029	the protein conformers	1008:1029	the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity	1008:1093	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	2	99	theme	O-glycans	459:467	arg1	structures					412:421	molecular structures	402:421	molecular structures	402:421	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	2	99	theme	O-glycans	459:467	arg1	heterogeneity					434:446	glycan heterogeneity	427:446	glycan heterogeneity	427:446	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	1	100	theme	key	323:325	arg1	roles					327:331	key roles	323:331	key roles	323:331	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry, and the S protein glycosylation plays key roles in altering the viral binding/function and infectivity.					
34328324	3	101	theme	structures	1203:1212	arg1	identification					1174:1187	unambiguous identification	1162:1187	unambiguous identification of the glycan structures and their glycosites	1162:1233	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	102	theme	complete	667:674	arg1	elucidation					687:697	the complete structural elucidation	663:697	the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites	663:1233	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	5	103	theme	proteoform-resolved	1448:1466	arg1	mapping					1468:1474	a high-resolution proteoform-resolved mapping	1430:1474	a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans	1430:1613	These findings demonstrate that this hybrid top-down MS approach can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
34328324	2	104	gly	heterogeneity	434:446	arg1	O-glycans					459:467	the new O-glycans	451:467	the new O-glycans found on the S protein regional-binding domain (S-RBD)	451:522	However, the molecular structures and glycan heterogeneity of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis by conventional bottom-up glycoproteomic approaches.					
34328324	3	105	theme	S-RBD	1038:1042	arg1	conformers					1020:1029	the protein conformers	1008:1029	the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity	1008:1093	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	3	106	theme	proteoforms	718:728	arg1	elucidation					687:697	the complete structural elucidation	663:697	the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites	663:1233	Here, we report the complete structural elucidation of intact O-glycan proteoforms through a hybrid native and denaturing top-down mass spectrometry (MS) approach employing both trapped ion mobility spectrometry (TIMS) quadrupole time-of-flight and ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR)-MS. Native top-down TIMS-MS/MS separates the protein conformers of the S-RBD to reveal their gas-phase structural heterogeneity, and top-down FTICR-MS/MS provides in-depth glycoform analysis for unambiguous identification of the glycan structures and their glycosites.					
34328324	6	107	gly	heterogeneity	1702:1714	arg1	O-glycoproteins					1771:1785	other O-glycoproteins	1765:1785	emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins	1719:1785	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
34328324	6	107	gly	heterogeneity	1702:1714	arg1	variants					1745:1752	emergent SARS-CoV-2 S-RBD variants	1719:1752	emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins	1719:1785	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants as well as other O-glycoproteins in general.					
32844281	6	0	theme	tag	1145:1147	arg1	concentration					1149:1161	tag concentration	1145:1161	tag concentration	1145:1161	Second, modifications in the INLIGHT™ derivatization step were evaluated including temperature optimization, solvent composition changes, reaction condition length and tag concentration.					
32844281	3	1	theme	biological	695:704	arg1	samples					706:712	biological samples	695:712	biological samples	695:712	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	7	2	theme	modified	1184:1191	arg1	method					1193:1198	the modified method	1180:1198	the modified method	1180:1198	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	1	3	theme	significant	259:269	arg1	challenges					271:280	significant challenges	259:280	significant challenges	259:280	The analysis of N-linked glycans using liquid chromatography and mass spectrometry (LC-MS) presents significant challenges, particularly owing to their hydrophilic nature.					
32844281	3	4	from	analysis	656:663	arg1	samples					706:712	biological samples	695:712	biological samples	695:712	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	7	5	theme	horseradish	1292:1302	arg1	peroxidase					1304:1313	horseradish peroxidase	1292:1313	horseradish peroxidase	1292:1313	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	9	6	theme	derivatized	1548:1558	arg1	analyses					1576:1583	the INLIGHT™ derivatized N-linked glycan analyses	1535:1583	the INLIGHT™ derivatized N-linked glycan analyses	1535:1583	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	3	7	from	N-	668:669	arg1	samples					706:712	biological samples	695:712	biological samples	695:712	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	7	8	link	N-linked	1261:1268	arg1	glycans					1270:1276	the detected N-linked glycans	1248:1276	the detected N-linked glycans in fetuin and horseradish peroxidase	1248:1313	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	1	9	theme	N-linked	175:182	arg1	glycans					184:190	N-linked glycans	175:190	N-linked glycans using liquid chromatography and mass spectrometry (LC-MS)	175:248	The analysis of N-linked glycans using liquid chromatography and mass spectrometry (LC-MS) presents significant challenges, particularly owing to their hydrophilic nature.					
32844281	9	10	theme	glycan	1569:1574	arg1	analyses					1576:1583	the INLIGHT™ derivatized N-linked glycan analyses	1535:1583	the INLIGHT™ derivatized N-linked glycan analyses	1535:1583	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	5	11	theme	PNGase	908:913	arg1	F					915:915	PNGase F	908:915	PNGase F	908:915	First, recombinant and nonrecombinant sources of PNGase F were compared to assess variations in the released glycans.					
32844281	0	12	link	N-linked	35:42	arg1	analysis					54:61	quantitative N-linked glycomics analysis	22:61	quantitative N-linked glycomics analysis using Individuality Normalization	22:95	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	0	13	theme	Tags	142:145	arg1	™					156:156	Isotopic Glycan Hydrazide Tags (INLIGHT)™	116:156	Isotopic Glycan Hydrazide Tags (INLIGHT)™	116:156	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	3	14	from	samples	706:712	arg1	analysis					656:663	the analysis	652:663	the analysis of N- and O-linked glycans in biological samples	652:712	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	1	15	theme	mass	224:227	arg1	LC-MS					243:247	LC-MS	243:247	LC-MS	243:247	The analysis of N-linked glycans using liquid chromatography and mass spectrometry (LC-MS) presents significant challenges, particularly owing to their hydrophilic nature.					
32844281	1	15	theme	mass	224:227	arg1	spectrometry					229:240	mass spectrometry	224:240	mass spectrometry (LC-MS)	224:248	The analysis of N-linked glycans using liquid chromatography and mass spectrometry (LC-MS) presents significant challenges, particularly owing to their hydrophilic nature.					
32844281	9	16	theme	reverse-phase	1607:1619	arg1	column					1626:1631	a C18 reverse-phase (RP) column	1601:1631	a C18 reverse-phase (RP) column with mobile phases typical of RPLC	1601:1666	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	9	16	theme	reverse-phase	1607:1619	arg1	setup					1525:1529	the optimal LC setup	1510:1529	the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses	1510:1583	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	6	17	theme	temperature	1060:1070	arg1	optimization					1072:1083	temperature optimization	1060:1083	temperature optimization	1060:1083	Second, modifications in the INLIGHT™ derivatization step were evaluated including temperature optimization, solvent composition changes, reaction condition length and tag concentration.					
32844281	9	18	theme	RP	1622:1623	arg1	column					1626:1631	a C18 reverse-phase (RP) column	1601:1631	a C18 reverse-phase (RP) column with mobile phases typical of RPLC	1601:1666	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	9	18	theme	RP	1622:1623	arg1	setup					1525:1529	the optimal LC setup	1510:1529	the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses	1510:1583	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	8	19	theme	low-abundance	1385:1397	arg1	Gal					1457:1459	(Gal)3	1456:1461	(Gal)3(GlcNAc)	1456:1469	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	8	19	theme	low-abundance	1385:1397	arg1	NeuAc					1444:1448	(Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1	1416:1450	(Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1	1416:1450	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	8	19	theme	low-abundance	1385:1397	arg1	glycans					1399:1405	low-abundance glycans	1385:1405	low-abundance glycans	1385:1405	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	0	20	theme	Individuality	69:81	arg1	Normalization					83:95	Individuality Normalization	69:95	Individuality Normalization	69:95	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	9	21	theme	mobile	1638:1643	arg1	phases					1645:1650	mobile phases	1638:1650	mobile phases typical of RPLC	1638:1666	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	4	22	theme	preparation	782:792	arg1	separability					753:764	separability	753:764	separability	753:764	To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated.					
32844281	4	22	theme	preparation	782:792	arg1	sensitivity					737:747	maximum sensitivity	729:747	maximum sensitivity	729:747	To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated.					
32844281	7	23	theme	N-linked	1261:1268	arg1	glycans					1270:1276	the detected N-linked glycans	1248:1276	the detected N-linked glycans in fetuin and horseradish peroxidase	1248:1313	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	6	24	theme	INLIGHT™	1006:1013	arg1	step					1030:1033	the INLIGHT™ derivatization step	1002:1033	the INLIGHT™ derivatization step	1002:1033	Second, modifications in the INLIGHT™ derivatization step were evaluated including temperature optimization, solvent composition changes, reaction condition length and tag concentration.					
32844281	9	25	theme	typical	1652:1658	arg1	phases					1645:1650	mobile phases	1638:1650	mobile phases typical of RPLC	1638:1666	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	8	26	theme	Fuc	1417:1419	arg1	GlcNAc					1429:1434	GlcNAc	1429:1434	GlcNAc	1429:1434	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	8	26	theme	Fuc	1417:1419	arg1	Gal					1423:1425	(Fuc)1(Gal)2	1416:1427	(Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1	1416:1450	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	0	27	theme	Glycan	125:130	arg1	INLIGHT					148:154	INLIGHT	148:154	INLIGHT	148:154	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	0	27	theme	Glycan	125:130	arg1	Tags					142:145	Isotopic Glycan Hydrazide Tags	116:145	Isotopic Glycan Hydrazide Tags (INLIGHT)™	116:156	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	6	28	theme	condition	1124:1132	arg1	length					1134:1139	reaction condition length	1115:1139	reaction condition length	1115:1139	Second, modifications in the INLIGHT™ derivatization step were evaluated including temperature optimization, solvent composition changes, reaction condition length and tag concentration.					
32844281	9	29	theme	RPLC	1663:1666	arg1	typical					1652:1658	typical	1652:1658	typical	1652:1658	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	9	30	link	N-linked	1560:1567	arg1	analyses					1576:1583	the INLIGHT™ derivatized N-linked glycan analyses	1535:1583	the INLIGHT™ derivatized N-linked glycan analyses	1535:1583	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	2	31	theme	detection	456:464	arg1	sensitivity					466:476	detection sensitivity	456:476	detection sensitivity	456:476	To address these difficulties, a variety of derivatization methods have been developed to facilitate improved ionization and detection sensitivity.					
32844281	8	32	theme	Man	1472:1474	arg1	NeuAc					1478:1482	5(Man)3(NeuAc)3	1470:1484	5(Man)3(NeuAc)3	1470:1484	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	6	33	from	modifications	985:997	arg1	step					1030:1033	the INLIGHT™ derivatization step	1002:1033	the INLIGHT™ derivatization step	1002:1033	Second, modifications in the INLIGHT™ derivatization step were evaluated including temperature optimization, solvent composition changes, reaction condition length and tag concentration.					
32844281	8	34	theme	Gal	1423:1425	arg1	NeuAc					1444:1448	(Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1	1416:1450	(Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1	1416:1450	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	7	35	theme	greater	1225:1231	arg1	areas					1238:1242	20-100 times greater peak areas	1212:1242	20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase	1212:1313	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	6	36	theme	composition	1094:1104	arg1	changes					1106:1112	solvent composition changes	1086:1112	solvent composition changes	1086:1112	Second, modifications in the INLIGHT™ derivatization step were evaluated including temperature optimization, solvent composition changes, reaction condition length and tag concentration.					
32844281	3	37	theme	N-	668:669	arg1	analysis					656:663	the analysis	652:663	the analysis of N- and O-linked glycans in biological samples	652:712	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	0	38	theme	N-linked	35:42	arg1	analysis					54:61	quantitative N-linked glycomics analysis	22:61	quantitative N-linked glycomics analysis using Individuality Normalization	22:95	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	8	39	dep	NeuAc	1444:1448	arg1	NeuAc					1478:1482	5(Man)3(NeuAc)3	1470:1484	5(Man)3(NeuAc)3	1470:1484	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	1	40	theme	hydrophilic	311:321	arg1	nature					323:328	their hydrophilic nature	305:328	their hydrophilic nature	305:328	The analysis of N-linked glycans using liquid chromatography and mass spectrometry (LC-MS) presents significant challenges, particularly owing to their hydrophilic nature.					
32844281	7	41	theme	standard	1333:1340	arg1	method					1342:1347	the standard method	1329:1347	the standard method	1329:1347	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	3	42	theme	O-linked	675:682	arg1	glycans					684:690	O-linked glycans	675:690	O-linked glycans	675:690	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	8	43	theme	Man	1438:1440	arg1	NeuAc					1444:1448	(Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1	1416:1450	(Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1	1416:1450	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	3	44	theme	Isotopic	547:554	arg1	INLIGHT					579:585	INLIGHT	579:585	INLIGHT	579:585	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	3	44	theme	Isotopic	547:554	arg1	Tags					573:576	Isotopic Glycan Hydrazide Tags	547:576	Isotopic Glycan Hydrazide Tags (INLIGHT)	547:586	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	9	45	theme	LC	1522:1523	arg1	column					1626:1631	a C18 reverse-phase (RP) column	1601:1631	a C18 reverse-phase (RP) column with mobile phases typical of RPLC	1601:1666	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	9	45	theme	LC	1522:1523	arg1	setup					1525:1529	the optimal LC setup	1510:1529	the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses	1510:1583	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	3	46	from	glycans	684:690	arg1	samples					706:712	biological samples	695:712	biological samples	695:712	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	3	47	with	Labeling	533:540	arg1	INLIGHT					579:585	INLIGHT	579:585	INLIGHT	579:585	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	3	47	with	Labeling	533:540	arg1	Tags					573:576	Isotopic Glycan Hydrazide Tags	547:576	Isotopic Glycan Hydrazide Tags (INLIGHT)	547:586	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	3	48	theme	Hydrazide	563:571	arg1	INLIGHT					579:585	INLIGHT	579:585	INLIGHT	579:585	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	3	48	theme	Hydrazide	563:571	arg1	Tags					573:576	Isotopic Glycan Hydrazide Tags	547:576	Isotopic Glycan Hydrazide Tags (INLIGHT)	547:586	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	5	49	theme	nonrecombinant	882:895	arg1	sources					897:903	First, recombinant and nonrecombinant sources	859:903	First, recombinant and nonrecombinant sources of PNGase F	859:915	First, recombinant and nonrecombinant sources of PNGase F were compared to assess variations in the released glycans.					
32844281	9	50	theme	N-linked	1560:1567	arg1	analyses					1576:1583	the INLIGHT™ derivatized N-linked glycan analyses	1535:1583	the INLIGHT™ derivatized N-linked glycan analyses	1535:1583	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	1	51	theme	glycans	184:190	arg1	analysis					163:170	The analysis	159:170	The analysis of N-linked glycans using liquid chromatography and mass spectrometry (LC-MS)	159:248	The analysis of N-linked glycans using liquid chromatography and mass spectrometry (LC-MS) presents significant challenges, particularly owing to their hydrophilic nature.					
32844281	1	52	theme	liquid	198:203	arg1	chromatography					205:218	liquid chromatography	198:218	liquid chromatography	198:218	The analysis of N-linked glycans using liquid chromatography and mass spectrometry (LC-MS) presents significant challenges, particularly owing to their hydrophilic nature.					
32844281	2	53	theme	derivatization	375:388	arg1	methods					390:396	derivatization methods	375:396	derivatization methods	375:396	To address these difficulties, a variety of derivatization methods have been developed to facilitate improved ionization and detection sensitivity.					
32844281	8	54	theme	glycans	1399:1405	arg1	identification					1367:1380	the identification	1363:1380	the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3,	1363:1485	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	8	54	theme	glycans	1399:1405	arg1	possible					1491:1498	possible	1491:1498	possible	1491:1498	Furthermore, the identification of low-abundance glycans, such as (Fuc)1(Gal)2(GlcNAc)4(Man)3(NeuAc)1 and (Gal)3(GlcNAc)5(Man)3(NeuAc)3, was possible.					
32844281	5	55	theme	recombinant	866:876	arg1	sources					897:903	First, recombinant and nonrecombinant sources	859:903	First, recombinant and nonrecombinant sources of PNGase F	859:915	First, recombinant and nonrecombinant sources of PNGase F were compared to assess variations in the released glycans.					
32844281	7	56	theme	detected	1252:1259	arg1	glycans					1270:1276	the detected N-linked glycans	1248:1276	the detected N-linked glycans in fetuin and horseradish peroxidase	1248:1313	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	3	57	theme	labeling	602:609	arg1	glycans					611:617	labeling glycans	602:617	labeling glycans	602:617	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	9	58	theme	C18	1603:1605	arg1	column					1626:1631	a C18 reverse-phase (RP) column	1601:1631	a C18 reverse-phase (RP) column with mobile phases typical of RPLC	1601:1666	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	9	58	theme	C18	1603:1605	arg1	setup					1525:1529	the optimal LC setup	1510:1529	the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses	1510:1583	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	7	59	from	glycans	1270:1276	arg1	fetuin					1281:1286	fetuin	1281:1286	fetuin	1281:1286	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	7	59	from	glycans	1270:1276	arg1	peroxidase					1304:1313	horseradish peroxidase	1292:1313	horseradish peroxidase	1292:1313	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	9	60	with	column	1626:1631	arg1	phases					1645:1650	mobile phases	1638:1650	mobile phases typical of RPLC	1638:1666	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	4	61	theme	maximum	729:735	arg1	sensitivity					737:747	maximum sensitivity	729:747	maximum sensitivity	729:747	To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated.					
32844281	5	62	theme	First	859:863	arg1	sources					897:903	First, recombinant and nonrecombinant sources	859:903	First, recombinant and nonrecombinant sources of PNGase F	859:915	First, recombinant and nonrecombinant sources of PNGase F were compared to assess variations in the released glycans.					
32844281	5	63	theme	F	915:915	arg1	sources					897:903	First, recombinant and nonrecombinant sources	859:903	First, recombinant and nonrecombinant sources of PNGase F	859:915	First, recombinant and nonrecombinant sources of PNGase F were compared to assess variations in the released glycans.					
32844281	4	64	dep	preparation	782:792	arg1	pipeline					807:814	pipeline	807:814	pipeline	807:814	To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated.					
32844281	3	65	used	utilized	640:647	arg2	One					479:481	One	479:481	One	479:481	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	3	65	used	utilized	640:647	arg2	Normalization					514:526	the Individuality Normalization	496:526	the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans	496:617	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	0	66	theme	Isotopic	116:123	arg1	INLIGHT					148:154	INLIGHT	148:154	INLIGHT	148:154	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	0	66	theme	Isotopic	116:123	arg1	Tags					142:145	Isotopic Glycan Hydrazide Tags	116:145	Isotopic Glycan Hydrazide Tags (INLIGHT)™	116:156	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	6	67	theme	derivatization	1015:1028	arg1	step					1030:1033	the INLIGHT™ derivatization step	1002:1033	the INLIGHT™ derivatization step	1002:1033	Second, modifications in the INLIGHT™ derivatization step were evaluated including temperature optimization, solvent composition changes, reaction condition length and tag concentration.					
32844281	4	68	theme	INLIGHT™	773:780	arg1	preparation					782:792	the INLIGHT™ preparation	769:792	the INLIGHT™ preparation	769:792	To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated.					
32844281	0	69	theme	Hydrazide	132:140	arg1	INLIGHT					148:154	INLIGHT	148:154	INLIGHT	148:154	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	0	69	theme	Hydrazide	132:140	arg1	Tags					142:145	Isotopic Glycan Hydrazide Tags	116:145	Isotopic Glycan Hydrazide Tags (INLIGHT)™	116:156	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	7	70	dep	resulted	1200:1207	arg1	compared					1315:1322	compared	1315:1322	compared with the standard method	1315:1347	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	7	71	theme	peak	1233:1236	arg1	areas					1238:1242	20-100 times greater peak areas	1212:1242	20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase	1212:1313	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	5	72	theme	released	959:966	arg1	glycans					968:974	the released glycans	955:974	the released glycans	955:974	First, recombinant and nonrecombinant sources of PNGase F were compared to assess variations in the released glycans.					
32844281	6	73	theme	reaction	1115:1122	arg1	length					1134:1139	reaction condition length	1115:1139	reaction condition length	1115:1139	Second, modifications in the INLIGHT™ derivatization step were evaluated including temperature optimization, solvent composition changes, reaction condition length and tag concentration.					
32844281	1	74	link	N-linked	175:182	arg1	glycans					184:190	N-linked glycans	175:190	N-linked glycans using liquid chromatography and mass spectrometry (LC-MS)	175:248	The analysis of N-linked glycans using liquid chromatography and mass spectrometry (LC-MS) presents significant challenges, particularly owing to their hydrophilic nature.					
32844281	2	75	theme	methods	390:396	arg1	variety					364:370	a variety	362:370	a variety of derivatization methods	362:396	To address these difficulties, a variety of derivatization methods have been developed to facilitate improved ionization and detection sensitivity.					
32844281	2	75	theme	methods	390:396	arg1	methods					390:396	derivatization methods	375:396	derivatization methods	375:396	To address these difficulties, a variety of derivatization methods have been developed to facilitate improved ionization and detection sensitivity.					
32844281	0	76	theme	quantitative	22:33	arg1	analysis					54:61	quantitative N-linked glycomics analysis	22:61	quantitative N-linked glycomics analysis using Individuality Normalization	22:95	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	4	77	theme	critical	825:832	arg1	steps					834:838	several critical steps	817:838	several critical steps	817:838	To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated.					
32844281	5	78	from	variations	941:950	arg1	glycans					968:974	the released glycans	955:974	the released glycans	955:974	First, recombinant and nonrecombinant sources of PNGase F were compared to assess variations in the released glycans.					
32844281	4	79	theme	analysis	798:805	arg1	separability					753:764	separability	753:764	separability	753:764	To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated.					
32844281	4	79	theme	analysis	798:805	arg1	sensitivity					737:747	maximum sensitivity	729:747	maximum sensitivity	729:747	To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated.					
32844281	2	80	theme	improved	432:439	arg1	ionization					441:450	improved ionization	432:450	improved ionization	432:450	To address these difficulties, a variety of derivatization methods have been developed to facilitate improved ionization and detection sensitivity.					
32844281	0	81	theme	glycomics	44:52	arg1	analysis					54:61	quantitative N-linked glycomics analysis	22:61	quantitative N-linked glycomics analysis using Individuality Normalization	22:95	Enhanced protocol for quantitative N-linked glycomics analysis using Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™.					
32844281	6	82	theme	solvent	1086:1092	arg1	changes					1106:1112	solvent composition changes	1086:1112	solvent composition changes	1086:1112	Second, modifications in the INLIGHT™ derivatization step were evaluated including temperature optimization, solvent composition changes, reaction condition length and tag concentration.					
32844281	7	83	theme	method	1193:1198	arg1	Optimization					1164:1175	Optimization	1164:1175	Optimization of the modified method	1164:1198	Optimization of the modified method resulted in 20-100 times greater peak areas for the detected N-linked glycans in fetuin and horseradish peroxidase compared with the standard method.					
32844281	3	84	theme	glycans	684:690	arg1	analysis					656:663	the analysis	652:663	the analysis of N- and O-linked glycans in biological samples	652:712	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	4	85	dep	sensitivity	737:747	arg1	the					725:727	the	725:727	the	725:727	To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated.					
32844281	3	86	link	O-linked	675:682	arg1	glycans					684:690	O-linked glycans	675:690	O-linked glycans	675:690	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	3	87	theme	Individuality	500:512	arg1	Normalization					514:526	the Individuality Normalization	496:526	the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans	496:617	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	3	87	theme	Individuality	500:512	arg1	One					479:481	One	479:481	One	479:481	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	9	88	theme	optimal	1514:1520	arg1	column					1626:1631	a C18 reverse-phase (RP) column	1601:1631	a C18 reverse-phase (RP) column with mobile phases typical of RPLC	1601:1666	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	9	88	theme	optimal	1514:1520	arg1	setup					1525:1529	the optimal LC setup	1510:1529	the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses	1510:1583	Finally, the optimal LC setup for the INLIGHT™ derivatized N-linked glycan analyses was found to be a C18 reverse-phase (RP) column with mobile phases typical of RPLC.					
32844281	4	89	theme	several	817:823	arg1	steps					834:838	several critical steps	817:838	several critical steps	817:838	To assess the maximum sensitivity and separability of the INLIGHT™ preparation and analysis pipeline, several critical steps were investigated.					
32844281	3	90	theme	Glycan	556:561	arg1	INLIGHT					579:585	INLIGHT	579:585	INLIGHT	579:585	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
32844281	3	90	theme	Glycan	556:561	arg1	Tags					573:576	Isotopic Glycan Hydrazide Tags	547:576	Isotopic Glycan Hydrazide Tags (INLIGHT)	547:586	One such method, the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ strategy for labeling glycans, has previously been utilized in the analysis of N- and O-linked glycans in biological samples.					
34543669	6	0	from	exposure	935:942	arg1	stage					926:930	the embryonic stage	912:930	the embryonic stage at exposure	912:942	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	7	1	theme	SNC	1122:1124	arg1	exposure					1110:1117	exposure	1110:1117	exposure of SNC	1110:1124	In situ hybridization analyses indicated that the five genes were expressed mainly in the head region; exposure of SNC suppressed alg2 and gnsb and enhanced b4galt2 and b3gat1a expression relative to controls on day 7.					
34543669	10	2	theme	medaka	1466:1471	arg1	embryo					1473:1478	the medaka embryo	1462:1478	the medaka embryo	1462:1478	In the medaka embryo, high mannose-type glycans were dominant, and SNC exposure altered glycan synthesis.					
34543669	4	3	theme	exposure	632:639	arg1	effects					617:623	the glycobiological effects	597:623	the glycobiological effects of SNC exposure on medaka embryogenesis	597:663	This study addressed the glycobiological effects of SNC exposure on medaka embryogenesis.					
34543669	10	4	from	dominant	1512:1519	arg1	embryo					1473:1478	the medaka embryo	1462:1478	the medaka embryo	1462:1478	In the medaka embryo, high mannose-type glycans were dominant, and SNC exposure altered glycan synthesis.					
34543669	4	5	theme	SNC	628:630	arg1	exposure					632:639	SNC exposure	628:639	SNC exposure	628:639	This study addressed the glycobiological effects of SNC exposure on medaka embryogenesis.					
34543669	6	6	theme	embryonic	916:924	arg1	stage					926:930	the embryonic stage	912:930	the embryonic stage at exposure	912:942	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	7	dep	genes	810:814	arg1	gnsb					823:826	gnsb	823:826	gnsb	823:826	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	7	dep	genes	810:814	arg1	b3gat2					851:856	b3gat2	851:856	b3gat2	851:856	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	7	dep	genes	810:814	arg1	genes					810:814	five glycan-related genes	790:814	five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2)	790:857	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	7	dep	genes	810:814	arg1	b4galt2					829:835	b4galt2	829:835	b4galt2	829:835	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	7	dep	genes	810:814	arg1	b3gat1a					838:844	b3gat1a	838:844	b3gat1a	838:844	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	7	dep	genes	810:814	arg1	alg2					817:820	alg2	817:820	alg2	817:820	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	4	8	theme	medaka	644:649	arg1	embryogenesis					651:663	medaka embryogenesis	644:663	medaka embryogenesis	644:663	This study addressed the glycobiological effects of SNC exposure on medaka embryogenesis.					
34543669	6	9	from	changes	891:897	arg1	stage					926:930	the embryonic stage	912:930	the embryonic stage at exposure	912:942	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	10	with	deformities	962:972	arg1	exposure					979:986	exposure	979:986	exposure at earlier stages	979:1004	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	11	theme	earlier	991:997	arg1	stages					999:1004	earlier stages	991:1004	earlier stages	991:1004	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	10	12	theme	SNC	1526:1528	arg1	exposure					1530:1537	SNC exposure	1526:1537	SNC exposure	1526:1537	In the medaka embryo, high mannose-type glycans were dominant, and SNC exposure altered glycan synthesis.					
34543669	6	13	from	stages	999:1004	arg1	exposure					979:986	exposure	979:986	exposure at earlier stages	979:1004	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	9	14	theme	glycan	1432:1437	arg1	analysis					1449:1456	glycan structure analysis	1432:1456	glycan structure analysis	1432:1456	The effects of SNC exposure on glycan synthesis were estimated by glycan structure analysis.					
34543669	2	15	theme	fish	287:290	arg1	exposure					275:282	chemical exposure	266:282	chemical exposure of fish during embryogenesis	266:311	In particular, chemical exposure of fish during embryogenesis leads to deformities and puts the population at risk.					
34543669	1	16	theme	environmental	169:181	arg1	pollution					183:191	environmental pollution	169:191	environmental pollution	169:191	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	7	17	theme	relative	1195:1202	arg1	b4galt2					1164:1170	b4galt2	1164:1170	b4galt2	1164:1170	In situ hybridization analyses indicated that the five genes were expressed mainly in the head region; exposure of SNC suppressed alg2 and gnsb and enhanced b4galt2 and b3gat1a expression relative to controls on day 7.					
34543669	5	18	theme	medaka	684:689	arg1	embryos					691:697	medaka embryos	684:697	medaka embryos	684:697	After exposure of medaka embryos to SNC, deformities such as small heads and deformed eyes were observed.					
34543669	7	19	theme	b3gat1a	1176:1182	arg1	expression					1184:1193	b3gat1a expression	1176:1193	b3gat1a expression	1176:1193	In situ hybridization analyses indicated that the five genes were expressed mainly in the head region; exposure of SNC suppressed alg2 and gnsb and enhanced b4galt2 and b3gat1a expression relative to controls on day 7.					
34543669	12	20	theme	disordered	1741:1750	arg1	glycosylation					1752:1764	disordered glycosylation	1741:1764	disordered glycosylation	1741:1764	Thus, SNC exposure causes embryonic deformities in medaka embryos through disordered glycosylation.					
34543669	11	21	theme	early	1630:1634	arg1	stage					1636:1640	an early stage	1627:1640	an early stage of medaka embryogenesis	1627:1664	The alteration was more significant when exposure occurred at an early stage of medaka embryogenesis.					
34543669	5	22	theme	embryos	691:697	arg1	exposure					672:679	exposure	672:679	exposure of medaka embryos to SNC	672:704	After exposure of medaka embryos to SNC, deformities such as small heads and deformed eyes were observed.					
34543669	12	23	theme	embryonic	1693:1701	arg1	deformities					1703:1713	embryonic deformities	1693:1713	embryonic deformities in medaka embryos	1693:1731	Thus, SNC exposure causes embryonic deformities in medaka embryos through disordered glycosylation.					
34543669	9	24	from	effects	1370:1376	arg1	synthesis					1404:1412	glycan synthesis	1397:1412	glycan synthesis	1397:1412	The effects of SNC exposure on glycan synthesis were estimated by glycan structure analysis.					
34543669	3	25	theme	proper	432:437	arg1	morphology					439:448	proper morphology	432:448	proper morphology in embryogenesis	432:465	Although glycans and glycosylation are known to be important for proper morphology in embryogenesis, little glycobiology-based research has examined morphological disorders caused by environmental pollutants.					
34543669	4	26	from	effects	617:623	arg1	embryogenesis					651:663	medaka embryogenesis	644:663	medaka embryogenesis	644:663	This study addressed the glycobiological effects of SNC exposure on medaka embryogenesis.					
34543669	4	27	theme	glycobiological	601:615	arg1	effects					617:623	the glycobiological effects	597:623	the glycobiological effects of SNC exposure on medaka embryogenesis	597:663	This study addressed the glycobiological effects of SNC exposure on medaka embryogenesis.					
34543669	7	28	theme	head	1097:1100	arg1	region					1102:1107	the head region	1093:1107	the head region	1093:1107	In situ hybridization analyses indicated that the five genes were expressed mainly in the head region; exposure of SNC suppressed alg2 and gnsb and enhanced b4galt2 and b3gat1a expression relative to controls on day 7.					
34543669	9	29	theme	exposure	1385:1392	arg1	effects					1370:1376	The effects	1366:1376	The effects of SNC exposure on glycan synthesis	1366:1412	The effects of SNC exposure on glycan synthesis were estimated by glycan structure analysis.					
34543669	1	30	theme	Silver	100:105	arg1	nanocolloids					136:147	silver nanocolloids	129:147	silver nanocolloids (SNC)	129:153	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	1	30	theme	Silver	100:105	arg1	nanomaterials					107:119	Silver nanomaterials	100:119	Silver nanomaterials such as silver nanocolloids (SNC)	100:153	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	0	31	theme	nanocolloids	19:30	arg1	Exposure					0:7	Exposure	0:7	Exposure of silver nanocolloids	0:30	Exposure of silver nanocolloids causes glycosylation disorders and embryonic deformities in medaka.					
34543669	12	32	theme	medaka	1718:1723	arg1	embryos					1725:1731	medaka embryos	1718:1731	medaka embryos	1718:1731	Thus, SNC exposure causes embryonic deformities in medaka embryos through disordered glycosylation.					
34543669	5	33	theme	deformed	743:750	arg1	eyes					752:755	deformed eyes	743:755	deformed eyes	743:755	After exposure of medaka embryos to SNC, deformities such as small heads and deformed eyes were observed.					
34543669	10	34	from	embryo	1473:1478	arg1	dominant					1512:1519	dominant	1512:1519	dominant	1512:1519	In the medaka embryo, high mannose-type glycans were dominant, and SNC exposure altered glycan synthesis.					
34543669	3	35	theme	glycobiology-based	475:492	arg1	research					494:501	little glycobiology-based research	468:501	little glycobiology-based research	468:501	Although glycans and glycosylation are known to be important for proper morphology in embryogenesis, little glycobiology-based research has examined morphological disorders caused by environmental pollutants.					
34543669	0	36	theme	silver	12:17	arg1	nanocolloids					19:30	silver nanocolloids	12:30	silver nanocolloids	12:30	Exposure of silver nanocolloids causes glycosylation disorders and embryonic deformities in medaka.					
34543669	1	37	contain	have	197:200	arg1	nanocolloids					136:147	silver nanocolloids	129:147	silver nanocolloids (SNC)	129:153	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	1	37	contain	have	197:200	arg2	effects					221:227	adverse ecological effects	202:227	adverse ecological effects	202:227	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	1	37	contain	have	197:200	arg1	nanomaterials					107:119	Silver nanomaterials	100:119	Silver nanomaterials such as silver nanocolloids (SNC)	100:153	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	1	38	theme	ecological	210:219	arg1	effects					221:227	adverse ecological effects	202:227	adverse ecological effects	202:227	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	0	39	theme	glycosylation	39:51	arg1	disorders					53:61	glycosylation disorders	39:61	glycosylation disorders	39:61	Exposure of silver nanocolloids causes glycosylation disorders and embryonic deformities in medaka.					
34543669	8	40	theme	RNA	1250:1252	arg1	overexpression					1254:1267	RNA overexpression	1250:1267	RNA overexpression	1250:1267	Loss (siRNA)- and gain (RNA overexpression)-of-function experiments confirmed that alg2, gnsb, and b4galt2 are essential for embryogenesis.					
34543669	12	41	from	deformities	1703:1713	arg1	embryos					1725:1731	medaka embryos	1718:1731	medaka embryos	1718:1731	Thus, SNC exposure causes embryonic deformities in medaka embryos through disordered glycosylation.					
34543669	7	42	dep	In	1007:1008	arg1	situ					1010:1013	situ	1010:1013	situ	1010:1013	In situ hybridization analyses indicated that the five genes were expressed mainly in the head region; exposure of SNC suppressed alg2 and gnsb and enhanced b4galt2 and b3gat1a expression relative to controls on day 7.					
34543669	9	43	theme	structure	1439:1447	arg1	analysis					1449:1456	glycan structure analysis	1432:1456	glycan structure analysis	1432:1456	The effects of SNC exposure on glycan synthesis were estimated by glycan structure analysis.					
34543669	9	44	theme	glycan	1397:1402	arg1	synthesis					1404:1412	glycan synthesis	1397:1412	glycan synthesis	1397:1412	The effects of SNC exposure on glycan synthesis were estimated by glycan structure analysis.					
34543669	6	45	theme	severe	955:960	arg1	deformities					962:972	more severe deformities	950:972	more severe deformities with exposure at earlier stages	950:1004	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	9	46	theme	SNC	1381:1383	arg1	exposure					1385:1392	SNC exposure	1381:1392	SNC exposure	1381:1392	The effects of SNC exposure on glycan synthesis were estimated by glycan structure analysis.					
34543669	11	47	theme	medaka	1645:1650	arg1	embryogenesis					1652:1664	medaka embryogenesis	1645:1664	medaka embryogenesis	1645:1664	The alteration was more significant when exposure occurred at an early stage of medaka embryogenesis.					
34543669	3	48	theme	little	468:473	arg1	research					494:501	little glycobiology-based research	468:501	little glycobiology-based research	468:501	Although glycans and glycosylation are known to be important for proper morphology in embryogenesis, little glycobiology-based research has examined morphological disorders caused by environmental pollutants.					
34543669	10	49	theme	mannose-type	1486:1497	arg1	glycans					1499:1505	high mannose-type glycans	1481:1505	high mannose-type glycans	1481:1505	In the medaka embryo, high mannose-type glycans were dominant, and SNC exposure altered glycan synthesis.					
34543669	7	50	from	controls	1207:1214	arg1	day					1219:1221	day 7	1219:1223	day 7	1219:1223	In situ hybridization analyses indicated that the five genes were expressed mainly in the head region; exposure of SNC suppressed alg2 and gnsb and enhanced b4galt2 and b3gat1a expression relative to controls on day 7.					
34543669	6	51	theme	genes	810:814	arg1	expression					776:785	The expression	772:785	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2)	772:857	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	3	52	theme	morphological	516:528	arg1	disorders					530:538	morphological disorders	516:538	morphological disorders caused by environmental pollutants	516:573	Although glycans and glycosylation are known to be important for proper morphology in embryogenesis, little glycobiology-based research has examined morphological disorders caused by environmental pollutants.					
34543669	5	53	theme	small	727:731	arg1	heads					733:737	small heads	727:737	small heads	727:737	After exposure of medaka embryos to SNC, deformities such as small heads and deformed eyes were observed.					
34543669	6	54	theme	glycan-related	795:808	arg1	gnsb					823:826	gnsb	823:826	gnsb	823:826	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	54	theme	glycan-related	795:808	arg1	b3gat2					851:856	b3gat2	851:856	b3gat2	851:856	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	54	theme	glycan-related	795:808	arg1	genes					810:814	five glycan-related genes	790:814	five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2)	790:857	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	54	theme	glycan-related	795:808	arg1	b4galt2					829:835	b4galt2	829:835	b4galt2	829:835	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	54	theme	glycan-related	795:808	arg1	b3gat1a					838:844	b3gat1a	838:844	b3gat1a	838:844	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	6	54	theme	glycan-related	795:808	arg1	alg2					817:820	alg2	817:820	alg2	817:820	The expression of five glycan-related genes (alg2, gnsb, b4galt2, b3gat1a, and b3gat2) was significantly altered, with changes depending on the embryonic stage at exposure, with more severe deformities with exposure at earlier stages.					
34543669	1	55	theme	silver	129:134	arg1	nanocolloids					136:147	silver nanocolloids	129:147	silver nanocolloids (SNC)	129:153	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	1	55	theme	silver	129:134	arg1	SNC					150:152	SNC	150:152	SNC	150:152	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	12	56	theme	SNC	1673:1675	arg1	exposure					1677:1684	SNC exposure	1673:1684	SNC exposure	1673:1684	Thus, SNC exposure causes embryonic deformities in medaka embryos through disordered glycosylation.					
34543669	7	57	theme	In	1007:1008	arg1	analyses					1029:1036	In situ hybridization analyses	1007:1036	In situ hybridization analyses	1007:1036	In situ hybridization analyses indicated that the five genes were expressed mainly in the head region; exposure of SNC suppressed alg2 and gnsb and enhanced b4galt2 and b3gat1a expression relative to controls on day 7.					
34543669	8	58	dep	-of-function	1269:1280	arg1	siRNA					1232:1236	siRNA	1232:1236	siRNA	1232:1236	Loss (siRNA)- and gain (RNA overexpression)-of-function experiments confirmed that alg2, gnsb, and b4galt2 are essential for embryogenesis.					
34543669	8	58	dep	-of-function	1269:1280	arg1	Loss					1226:1229	Loss	1226:1229	Loss	1226:1229	Loss (siRNA)- and gain (RNA overexpression)-of-function experiments confirmed that alg2, gnsb, and b4galt2 are essential for embryogenesis.					
34543669	8	58	dep	-of-function	1269:1280	arg1	gain					1244:1247	gain	1244:1247	gain	1244:1247	Loss (siRNA)- and gain (RNA overexpression)-of-function experiments confirmed that alg2, gnsb, and b4galt2 are essential for embryogenesis.					
34543669	8	58	dep	-of-function	1269:1280	arg1	overexpression					1254:1267	RNA overexpression	1250:1267	RNA overexpression	1250:1267	Loss (siRNA)- and gain (RNA overexpression)-of-function experiments confirmed that alg2, gnsb, and b4galt2 are essential for embryogenesis.					
34543669	7	59	theme	hybridization	1015:1027	arg1	analyses					1029:1036	In situ hybridization analyses	1007:1036	In situ hybridization analyses	1007:1036	In situ hybridization analyses indicated that the five genes were expressed mainly in the head region; exposure of SNC suppressed alg2 and gnsb and enhanced b4galt2 and b3gat1a expression relative to controls on day 7.					
34543669	8	60	theme	-of-function	1269:1280	arg1	experiments					1282:1292	Loss (siRNA)- and gain (RNA overexpression)-of-function experiments	1226:1292	Loss (siRNA)- and gain (RNA overexpression)-of-function experiments	1226:1292	Loss (siRNA)- and gain (RNA overexpression)-of-function experiments confirmed that alg2, gnsb, and b4galt2 are essential for embryogenesis.					
34543669	1	61	theme	aquatic	232:238	arg1	organisms					240:248	aquatic organisms	232:248	aquatic organisms	232:248	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	2	62	theme	chemical	266:273	arg1	exposure					275:282	chemical exposure	266:282	chemical exposure of fish during embryogenesis	266:311	In particular, chemical exposure of fish during embryogenesis leads to deformities and puts the population at risk.					
34543669	1	63	theme	adverse	202:208	arg1	effects					221:227	adverse ecological effects	202:227	adverse ecological effects	202:227	Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms.					
34543669	3	64	from	morphology	439:448	arg1	embryogenesis					453:465	embryogenesis	453:465	embryogenesis	453:465	Although glycans and glycosylation are known to be important for proper morphology in embryogenesis, little glycobiology-based research has examined morphological disorders caused by environmental pollutants.					
34543669	3	65	theme	environmental	550:562	arg1	pollutants					564:573	environmental pollutants	550:573	environmental pollutants	550:573	Although glycans and glycosylation are known to be important for proper morphology in embryogenesis, little glycobiology-based research has examined morphological disorders caused by environmental pollutants.					
34543669	10	66	theme	high	1481:1484	arg1	glycans					1499:1505	high mannose-type glycans	1481:1505	high mannose-type glycans	1481:1505	In the medaka embryo, high mannose-type glycans were dominant, and SNC exposure altered glycan synthesis.					
34543669	0	67	theme	embryonic	67:75	arg1	deformities					77:87	embryonic deformities	67:87	embryonic deformities	67:87	Exposure of silver nanocolloids causes glycosylation disorders and embryonic deformities in medaka.					
34543669	10	68	theme	glycan	1547:1552	arg1	synthesis					1554:1562	glycan synthesis	1547:1562	glycan synthesis	1547:1562	In the medaka embryo, high mannose-type glycans were dominant, and SNC exposure altered glycan synthesis.					
34543669	11	69	theme	embryogenesis	1652:1664	arg1	stage					1636:1640	an early stage	1627:1640	an early stage of medaka embryogenesis	1627:1664	The alteration was more significant when exposure occurred at an early stage of medaka embryogenesis.					
32102970	2	0	theme	motif-specific	619:632	arg1	FDR					656:658	FDR	656:658	FDR	656:658	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	0	theme	motif-specific	619:632	arg1	rate					650:653	motif-specific false discovery rate	619:653	motif-specific false discovery rate (FDR)	619:659	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	6	1	theme	N-linked	1152:1159	arg1	sets					1182:1185	N-linked deglycopeptides data sets	1152:1185	N-linked deglycopeptides data sets	1152:1185	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	5	2	gly	glycopeptides	1086:1098	arg2	glycopeptides					1086:1098	N-linked intact glycopeptides	1070:1098	N-linked intact glycopeptides	1070:1098	Both N-linked intact glycopeptides and deglycopeptides were analyzed by LC-MS/MS.					
32102970	6	3	gly	deglycopeptides	1266:1280	arg2	deglycopeptides					1266:1280	3328 unique N-linked deglycopeptides	1245:1280	3328 unique N-linked deglycopeptides	1245:1280	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	8	4	theme	glycopeptides	1561:1573	arg1	identification					1527:1540	identification	1527:1540	identification	1527:1540	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	8	4	theme	glycopeptides	1561:1573	arg1	construction					1510:1521	N-linked deglycopeptides library construction	1477:1521	N-linked deglycopeptides library construction	1477:1521	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	10	5	theme	glycopeptide	1985:1996	arg1	level					1998:2002	N-linked intact glycopeptide level	1969:2002	N-linked intact glycopeptide level	1969:2002	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	6	6	theme	unique	1250:1255	arg1	deglycopeptides					1266:1280	3328 unique N-linked deglycopeptides	1245:1280	3328 unique N-linked deglycopeptides	1245:1280	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	5	7	gly	deglycopeptides	1104:1118	arg2	deglycopeptides					1104:1118	deglycopeptides	1104:1118	deglycopeptides	1104:1118	Both N-linked intact glycopeptides and deglycopeptides were analyzed by LC-MS/MS.					
32102970	8	8	theme	deglycopeptides	1443:1457	arg1	spectra					1417:1423	The spectra	1413:1423	The spectra of these N-linked deglycopeptides	1413:1457	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	7	9	theme	N-linked	1313:1320	arg1	motifs					1336:1341	N-linked glycosylation motifs	1313:1341	N-linked glycosylation motifs (NXS/T/C/V, X≠P)	1313:1358	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	7	9	theme	N-linked	1313:1320	arg1	X≠P					1355:1357	X≠P	1355:1357	X≠P	1355:1357	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	4	10	theme	liquid	884:889	arg1	chromatography					891:904	hydrophilic interaction liquid chromatography	860:904	hydrophilic interaction liquid chromatography (HILIC)	860:912	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	4	10	theme	liquid	884:889	arg1	HILIC					907:911	HILIC	907:911	HILIC	907:911	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	3	11	theme	high-abundance	731:744	arg1	proteins					746:753	low-abundance and high-abundance proteins	713:753	low-abundance and high-abundance proteins	713:753	Serum proteins were firstly separated into low-abundance and high-abundance proteins by acetonitrile (ACN) precipitation.					
32102970	10	12	theme	1	1882:1882	arg1	%					1883:1883	%	1883:1883	%	1883:1883	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	4	13	link	N-linked	944:951	arg1	glycopeptides					960:972	the enriched N-linked intact glycopeptides	931:972	the enriched N-linked intact glycopeptides	931:972	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	2	14	theme	discovery	640:648	arg1	FDR					656:658	FDR	656:658	FDR	656:658	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	14	theme	discovery	640:648	arg1	rate					650:653	motif-specific false discovery rate	619:653	motif-specific false discovery rate (FDR)	619:659	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	6	15	theme	N-linked	1221:1228	arg1	glycosites					1230:1239	1699 N-linked glycosites	1216:1239	1699 N-linked glycosites	1216:1239	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	2	16	theme	N-linked	559:566	arg1	glycoproteins					568:580	N-linked glycoproteins	559:580	N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	559:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	1	17	theme	N-glycan	383:390	arg1	database					392:399	a complete serum N-glycan database	366:399	a complete serum N-glycan database	366:399	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	7	18	link	N-linked	1313:1320	arg1	motifs					1336:1341	N-linked glycosylation motifs	1313:1341	N-linked glycosylation motifs (NXS/T/C/V, X≠P)	1313:1358	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	7	18	link	N-linked	1313:1320	arg1	X≠P					1355:1357	X≠P	1355:1357	X≠P	1355:1357	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	1	19	theme	mass	232:235	arg1	LC-MS/MS					251:258	LC-MS/MS	251:258	LC-MS/MS	251:258	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	1	19	theme	mass	232:235	arg1	spectrometry					237:248	tandem mass spectrometry	225:248	tandem mass spectrometry (LC-MS/MS)	225:259	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	5	20	link	N-linked	1070:1077	arg1	glycopeptides					1086:1098	N-linked intact glycopeptides	1070:1098	N-linked intact glycopeptides	1070:1098	Both N-linked intact glycopeptides and deglycopeptides were analyzed by LC-MS/MS.					
32102970	2	21	theme	glycopeptides	540:552	arg1	identification					506:519	the identification	502:519	the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	502:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	8	22	theme	N-linked	1545:1552	arg1	glycopeptides					1561:1573	N-linked intact glycopeptides	1545:1573	N-linked intact glycopeptides	1545:1573	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	8	23	link	N-linked	1545:1552	arg1	glycopeptides					1561:1573	N-linked intact glycopeptides	1545:1573	N-linked intact glycopeptides	1545:1573	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	10	24	theme	N-linked	1749:1756	arg1	glycoproteins					1758:1770	526 N-linked glycoproteins	1745:1770	526 N-linked glycoproteins	1745:1770	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	9	25	theme	739	1598:1600	arg1	masses					1611:1616	739 N-glycan masses	1598:1616	739 N-glycan masses	1598:1616	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	0	26	from	Serum	69:73	arg1	Identification					12:25	Large-scale Identification	0:25	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.	0:125	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	1	27	theme	existence	409:417	arg1	range					319:323	the wide dynamic range	302:323	the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms	302:432	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	10	28	theme	serum	1921:1925	arg1	N-glycoproteome					1927:1941	the most in-depth serum N-glycoproteome	1903:1941	the most in-depth serum N-glycoproteome identified by LC-MS/MS	1903:1964	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	1	29	theme	Large-scale	127:137	arg1	identification					139:152	Large-scale identification	127:152	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum	127:274	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	8	30	link	N-linked	1434:1441	arg1	deglycopeptides					1443:1457	these N-linked deglycopeptides	1428:1457	these N-linked deglycopeptides	1428:1457	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	1	31	gly	glycopeptides	173:185	arg2	glycopeptides					173:185	N-linked intact glycopeptides	157:185	N-linked intact glycopeptides	157:185	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	1	32	theme	proteoforms	422:432	arg1	abundances					342:351	serum protein abundances	328:351	serum protein abundances	328:351	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	1	32	theme	proteoforms	422:432	arg1	lack					358:361	the lack	354:361	the lack of a complete serum N-glycan database	354:399	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	1	32	theme	proteoforms	422:432	arg1	existence					409:417	the existence	405:417	the existence of proteoforms	405:432	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	10	33	theme	N-linked	1969:1976	arg1	level					1998:2002	N-linked intact glycopeptide level	1969:2002	N-linked intact glycopeptide level	1969:2002	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	9	34	used	utilized	1638:1645	arg2	database					1578:1585	A database	1576:1585	A database containing 739 N-glycan masses	1576:1616	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	4	35	gly	glycopeptides	960:972	arg2	glycopeptides					960:972	the enriched N-linked intact glycopeptides	931:972	the enriched N-linked intact glycopeptides	931:972	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	4	36	theme	PNGase	1017:1022	arg1	F					1014:1014	Peptide-N-Glycosidase F	992:1014	Peptide-N-Glycosidase F (PNGase F)	992:1025	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	4	36	theme	PNGase	1017:1022	arg1	F					1024:1024	PNGase F	1017:1024	PNGase F	1017:1024	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	4	37	theme	intact	953:958	arg1	glycopeptides					960:972	the enriched N-linked intact glycopeptides	931:972	the enriched N-linked intact glycopeptides	931:972	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	7	38	link	N-linked	1387:1394	arg1	deglycopeptides					1396:1410	the N-linked deglycopeptides	1383:1410	the N-linked deglycopeptides	1383:1410	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	1	39	theme	abundances	342:351	arg1	range					319:323	the wide dynamic range	302:323	the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms	302:432	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	1	40	theme	intact	166:171	arg1	glycopeptides					173:185	N-linked intact glycopeptides	157:185	N-linked intact glycopeptides	157:185	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	6	41	gly	deglycopeptides	1161:1175	arg2	deglycopeptides					1161:1175	N-linked deglycopeptides data sets	1152:1185	N-linked deglycopeptides data sets	1152:1185	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	0	42	theme	HILIC	81:85	arg1	Enrichment					87:96	HILIC Enrichment and Spectral Library Search	81:124	Enrichment	87:96	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	10	43	theme	N-linked	1806:1813	arg1	glycopeptides					1822:1834	22,677 N-linked intact glycopeptides	1799:1834	22,677 N-linked intact glycopeptides	1799:1834	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	2	44	gly	glycopeptides	540:552	arg1	glycoproteins					568:580	N-linked glycoproteins	559:580	N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	559:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	44	gly	glycopeptides	540:552	arg2	glycopeptides					540:552	N-linked intact glycopeptides	524:552	N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	524:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	45	theme	N-linked	524:531	arg1	glycopeptides					540:552	N-linked intact glycopeptides	524:552	N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	524:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	10	46	gly	glycoproteins	1758:1770	arg1	glycoproteins					1758:1770	526 N-linked glycoproteins	1745:1770	526 N-linked glycoproteins	1745:1770	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	6	47	link	N-linked	1221:1228	arg1	glycosites					1230:1239	1699 N-linked glycosites	1216:1239	1699 N-linked glycosites	1216:1239	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	10	48	dep	N-linked	1806:1813	arg1	intact					1815:1820	intact	1815:1820	intact	1815:1820	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	8	49	used	utilized	1464:1471	arg2	spectra					1417:1423	The spectra	1413:1423	The spectra of these N-linked deglycopeptides	1413:1457	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	0	50	theme	Library	111:117	arg1	Search					119:124	HILIC Enrichment and Spectral Library Search	81:124	Search	119:124	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	1	51	theme	complete	368:375	arg1	database					392:399	a complete serum N-glycan database	366:399	a complete serum N-glycan database	366:399	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	7	52	gly	deglycopeptides	1396:1410	arg2	deglycopeptides					1396:1410	the N-linked deglycopeptides	1383:1410	the N-linked deglycopeptides	1383:1410	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	7	53	used	used	1365:1368	arg2	types					1304:1308	Four types	1299:1308	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P)	1299:1358	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	0	54	theme	Large-scale	0:10	arg1	Identification					12:25	Large-scale Identification	0:25	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.	0:125	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	7	55	theme	N-linked	1387:1394	arg1	deglycopeptides					1396:1410	the N-linked deglycopeptides	1383:1410	the N-linked deglycopeptides	1383:1410	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	1	56	theme	wide	306:309	arg1	range					319:323	the wide dynamic range	302:323	the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms	302:432	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	9	57	theme	intact	1713:1718	arg1	glycopeptides					1720:1732	N-linked intact glycopeptides	1704:1732	N-linked intact glycopeptides	1704:1732	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	2	58	theme	search	470:475	arg1	method					477:482	a spectral library search method	451:482	a spectral library search method	451:482	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	1	59	link	N-linked	157:164	arg1	glycopeptides					173:185	N-linked intact glycopeptides	157:185	N-linked intact glycopeptides	157:185	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	8	60	theme	N-linked	1477:1484	arg1	construction					1510:1521	N-linked deglycopeptides library construction	1477:1521	N-linked deglycopeptides library construction	1477:1521	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	0	61	theme	Intact	39:44	arg1	Glycopeptides					46:58	N-linked Intact Glycopeptides	30:58	N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search	30:124	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	2	62	theme	intact	533:538	arg1	glycopeptides					540:552	N-linked intact glycopeptides	524:552	N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	524:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	63	theme	spectral	453:460	arg1	method					477:482	a spectral library search method	451:482	a spectral library search method	451:482	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	10	64	link	N-linked	1778:1785	arg1	glycosites					1787:1796	1036 N-linked glycosites	1773:1796	1036 N-linked glycosites	1773:1796	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	4	65	theme	hydrophilic	860:870	arg1	chromatography					891:904	hydrophilic interaction liquid chromatography	860:904	hydrophilic interaction liquid chromatography (HILIC)	860:912	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	4	65	theme	hydrophilic	860:870	arg1	HILIC					907:911	HILIC	907:911	HILIC	907:911	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	10	66	theme	N-glycan	1844:1851	arg1	masses					1853:1858	738 N-glycan masses	1840:1858	738 N-glycan masses	1840:1858	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	4	67	gly	glycopeptides	829:841	arg2	glycopeptides					829:841	the N-linked intact glycopeptides	809:841	the N-linked intact glycopeptides	809:841	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	1	68	theme	serum	328:332	arg1	abundances					342:351	serum protein abundances	328:351	serum protein abundances	328:351	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	5	69	theme	intact	1079:1084	arg1	glycopeptides					1086:1098	N-linked intact glycopeptides	1070:1098	N-linked intact glycopeptides	1070:1098	Both N-linked intact glycopeptides and deglycopeptides were analyzed by LC-MS/MS.					
32102970	6	70	theme	764	1188:1190	arg1	glycoproteins					1201:1213	764 N-linked glycoproteins	1188:1213	764 N-linked glycoproteins	1188:1213	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	4	71	theme	N-linked	813:820	arg1	glycopeptides					829:841	the N-linked intact glycopeptides	809:841	the N-linked intact glycopeptides	809:841	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	9	72	gly	glycopeptides	1720:1732	arg2	glycopeptides					1720:1732	N-linked intact glycopeptides	1704:1732	N-linked intact glycopeptides	1704:1732	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	10	73	link	N-linked	1806:1813	arg1	glycopeptides					1822:1834	22,677 N-linked intact glycopeptides	1799:1834	22,677 N-linked intact glycopeptides	1799:1834	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	8	74	gly	deglycopeptides	1486:1500	arg2	deglycopeptides					1486:1500	N-linked deglycopeptides library construction	1477:1521	N-linked deglycopeptides library construction	1477:1521	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	4	75	gly	deglycopeptides	1048:1062	arg2	deglycopeptides					1048:1062	N-linked deglycopeptides	1039:1062	N-linked deglycopeptides	1039:1062	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	6	76	theme	deglycopeptides	1161:1175	arg1	sets					1182:1185	N-linked deglycopeptides data sets	1152:1185	N-linked deglycopeptides data sets	1152:1185	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	2	77	with	glycoproteins	568:580	arg1	FDR					656:658	FDR	656:658	FDR	656:658	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	77	with	glycoproteins	568:580	arg1	rate					650:653	motif-specific false discovery rate	619:653	motif-specific false discovery rate (FDR)	619:659	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	77	with	glycoproteins	568:580	arg1	target-decoy					602:613	target-decoy	602:613	target-decoy	602:613	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	0	78	link	N-linked	30:37	arg1	Glycopeptides					46:58	N-linked Intact Glycopeptides	30:58	N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search	30:124	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	7	79	theme	glycosylation	1322:1334	arg1	motifs					1336:1341	N-linked glycosylation motifs	1313:1341	N-linked glycosylation motifs (NXS/T/C/V, X≠P)	1313:1358	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	7	79	theme	glycosylation	1322:1334	arg1	X≠P					1355:1357	X≠P	1355:1357	X≠P	1355:1357	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	2	80	from	glycoproteins	568:580	arg1	identification					506:519	the identification	502:519	the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	502:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	80	from	glycoproteins	568:580	arg1	glycopeptides					540:552	N-linked intact glycopeptides	524:552	N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	524:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	80	from	glycoproteins	568:580	arg1	serum					591:595	human serum	585:595	human serum	585:595	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	8	81	theme	library	1502:1508	arg1	construction					1510:1521	N-linked deglycopeptides library construction	1477:1521	N-linked deglycopeptides library construction	1477:1521	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	3	82	theme	acetonitrile	758:769	arg1	precipitation					777:789	acetonitrile (ACN) precipitation	758:789	acetonitrile (ACN) precipitation	758:789	Serum proteins were firstly separated into low-abundance and high-abundance proteins by acetonitrile (ACN) precipitation.					
32102970	10	83	theme	%	1883:1883	arg1	FDR					1885:1887	1% FDR	1882:1887	1% FDR	1882:1887	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	8	84	gly	glycopeptides	1561:1573	arg2	glycopeptides					1561:1573	N-linked intact glycopeptides	1545:1573	N-linked intact glycopeptides	1545:1573	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	1	85	theme	liquid	190:195	arg1	chromatography					197:210	liquid chromatography	190:210	liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum	190:274	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	4	86	theme	interaction	872:882	arg1	chromatography					891:904	hydrophilic interaction liquid chromatography	860:904	hydrophilic interaction liquid chromatography (HILIC)	860:912	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	4	86	theme	interaction	872:882	arg1	HILIC					907:911	HILIC	907:911	HILIC	907:911	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	2	87	link	N-linked	559:566	arg1	glycoproteins					568:580	N-linked glycoproteins	559:580	N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	559:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	88	dep	target-decoy	602:613	arg1	control					661:667	control	661:667	control	661:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	10	89	theme	in-depth	1912:1919	arg1	N-glycoproteome					1927:1941	the most in-depth serum N-glycoproteome	1903:1941	the most in-depth serum N-glycoproteome identified by LC-MS/MS	1903:1964	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	4	90	dep	N-linked	813:820	arg1	intact					822:827	intact	822:827	intact	822:827	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	2	91	theme	false	634:638	arg1	FDR					656:658	FDR	656:658	FDR	656:658	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	91	theme	false	634:638	arg1	rate					650:653	motif-specific false discovery rate	619:653	motif-specific false discovery rate (FDR)	619:659	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	6	92	gly	glycoproteins	1201:1213	arg1	glycoproteins					1201:1213	764 N-linked glycoproteins	1188:1213	764 N-linked glycoproteins	1188:1213	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	10	93	link	N-linked	1749:1756	arg1	glycoproteins					1758:1770	526 N-linked glycoproteins	1745:1770	526 N-linked glycoproteins	1745:1770	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	1	94	theme	tandem	225:230	arg1	LC-MS/MS					251:258	LC-MS/MS	251:258	LC-MS/MS	251:258	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	1	94	theme	tandem	225:230	arg1	spectrometry					237:248	tandem mass spectrometry	225:248	tandem mass spectrometry (LC-MS/MS)	225:259	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	4	95	theme	N-linked	944:951	arg1	glycopeptides					960:972	the enriched N-linked intact glycopeptides	931:972	the enriched N-linked intact glycopeptides	931:972	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	8	96	theme	intact	1554:1559	arg1	glycopeptides					1561:1573	N-linked intact glycopeptides	1545:1573	N-linked intact glycopeptides	1545:1573	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	9	97	theme	spectral	1654:1661	arg1	search					1671:1676	spectral library search	1654:1676	spectral library search for the identification of N-linked intact glycopeptides	1654:1732	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	1	98	theme	database	392:399	arg1	abundances					342:351	serum protein abundances	328:351	serum protein abundances	328:351	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	1	98	theme	database	392:399	arg1	lack					358:361	the lack	354:361	the lack of a complete serum N-glycan database	354:399	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	1	98	theme	database	392:399	arg1	existence					409:417	the existence	405:417	the existence of proteoforms	405:432	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	10	99	gly	glycopeptide	1985:1996	arg2	glycopeptide					1985:1996	N-linked intact glycopeptide level	1969:2002	N-linked intact glycopeptide level	1969:2002	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	4	100	theme	glycopeptides	960:972	arg1	portion					920:926	a portion	918:926	a portion of the enriched N-linked intact glycopeptides	918:972	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	10	101	gly	glycopeptides	1822:1834	arg2	glycopeptides					1822:1834	22,677 N-linked intact glycopeptides	1799:1834	22,677 N-linked intact glycopeptides	1799:1834	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	6	102	gly	glycosites	1230:1239	arg2	glycosites					1230:1239	1699 N-linked glycosites	1216:1239	1699 N-linked glycosites	1216:1239	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	6	103	link	N-linked	1257:1264	arg1	deglycopeptides					1266:1280	3328 unique N-linked deglycopeptides	1245:1280	3328 unique N-linked deglycopeptides	1245:1280	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	9	104	theme	N-glycan	1602:1609	arg1	masses					1611:1616	739 N-glycan masses	1598:1616	739 N-glycan masses	1598:1616	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	2	105	theme	human	585:589	arg1	serum					591:595	human serum	585:595	human serum	585:595	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	2	106	from	identification	506:519	arg1	glycoproteins					568:580	N-linked glycoproteins	559:580	N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	559:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	10	107	gly	glycosites	1787:1796	arg2	glycosites					1787:1796	1036 N-linked glycosites	1773:1796	1036 N-linked glycosites	1773:1796	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	10	108	theme	intact	1978:1983	arg1	level					1998:2002	N-linked intact glycopeptide level	1969:2002	N-linked intact glycopeptide level	1969:2002	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	2	109	gly	glycoproteins	568:580	arg1	glycoproteins					568:580	N-linked glycoproteins	559:580	N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	559:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	1	110	theme	N-linked	157:164	arg1	glycopeptides					173:185	N-linked intact glycopeptides	157:185	N-linked intact glycopeptides	157:185	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	8	111	link	N-linked	1477:1484	arg1	construction					1510:1521	N-linked deglycopeptides library construction	1477:1521	N-linked deglycopeptides library construction	1477:1521	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	6	112	theme	N-linked	1257:1264	arg1	deglycopeptides					1266:1280	3328 unique N-linked deglycopeptides	1245:1280	3328 unique N-linked deglycopeptides	1245:1280	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	2	113	link	N-linked	524:531	arg1	glycopeptides					540:552	N-linked intact glycopeptides	524:552	N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control	524:667	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	1	114	theme	glycopeptides	173:185	arg1	identification					139:152	Large-scale identification	127:152	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum	127:274	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	0	115	from	Identification	12:25	arg1	Serum					69:73	Human Serum	63:73	Human Serum using HILIC Enrichment and Spectral Library Search	63:124	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	0	116	theme	Spectral	102:109	arg1	Search					119:124	HILIC Enrichment and Spectral Library Search	81:124	Search	119:124	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	1	117	theme	lack	358:361	arg1	range					319:323	the wide dynamic range	302:323	the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms	302:432	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	9	118	theme	library	1663:1669	arg1	search					1671:1676	spectral library search	1654:1676	spectral library search for the identification of N-linked intact glycopeptides	1654:1732	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	10	119	link	N-linked	1969:1976	arg1	level					1998:2002	N-linked intact glycopeptide level	1969:2002	N-linked intact glycopeptide level	1969:2002	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	4	120	theme	Peptide-N-Glycosidase	992:1012	arg1	F					1014:1014	Peptide-N-Glycosidase F	992:1014	Peptide-N-Glycosidase F (PNGase F)	992:1025	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	4	120	theme	Peptide-N-Glycosidase	992:1012	arg1	F					1024:1024	PNGase F	1017:1024	PNGase F	1017:1024	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	0	121	from	Glycopeptides	46:58	arg1	Serum					69:73	Human Serum	63:73	Human Serum using HILIC Enrichment and Spectral Library Search	63:124	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	4	122	link	N-linked	1039:1046	arg1	deglycopeptides					1048:1062	N-linked deglycopeptides	1039:1062	N-linked deglycopeptides	1039:1062	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	10	123	theme	N-linked	1778:1785	arg1	glycosites					1787:1796	1036 N-linked glycosites	1773:1796	1036 N-linked glycosites	1773:1796	In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.					
32102970	1	124	theme	serum	377:381	arg1	database					392:399	a complete serum N-glycan database	366:399	a complete serum N-glycan database	366:399	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	8	125	theme	N-linked	1434:1441	arg1	deglycopeptides					1443:1457	these N-linked deglycopeptides	1428:1457	these N-linked deglycopeptides	1428:1457	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	0	126	theme	N-linked	30:37	arg1	Glycopeptides					46:58	N-linked Intact Glycopeptides	30:58	N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search	30:124	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	9	127	theme	N-linked	1704:1711	arg1	glycopeptides					1720:1732	N-linked intact glycopeptides	1704:1732	N-linked intact glycopeptides	1704:1732	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	7	128	theme	motifs	1336:1341	arg1	types					1304:1308	Four types	1299:1308	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P)	1299:1358	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	0	129	theme	Glycopeptides	46:58	arg1	Identification					12:25	Large-scale Identification	0:25	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.	0:125	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	1	130	theme	dynamic	311:317	arg1	range					319:323	the wide dynamic range	302:323	the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms	302:432	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	7	131	gly	glycosylation	1322:1334	arg2	X≠P					1355:1357	X≠P	1355:1357	X≠P	1355:1357	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	7	131	gly	glycosylation	1322:1334	arg2	motifs					1336:1341	N-linked glycosylation motifs	1313:1341	N-linked glycosylation motifs (NXS/T/C/V, X≠P)	1313:1358	Four types of N-linked glycosylation motifs (NXS/T/C/V, X≠P) were used to recognize the N-linked deglycopeptides.					
32102970	9	132	theme	glycopeptides	1720:1732	arg1	identification					1686:1699	the identification	1682:1699	the identification of N-linked intact glycopeptides	1682:1732	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	4	133	link	N-linked	813:820	arg1	glycopeptides					829:841	the N-linked intact glycopeptides	809:841	the N-linked intact glycopeptides	809:841	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	2	134	theme	library	462:468	arg1	method					477:482	a spectral library search method	451:482	a spectral library search method	451:482	In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control.					
32102970	6	135	link	N-linked	1192:1199	arg1	glycoproteins					1201:1213	764 N-linked glycoproteins	1188:1213	764 N-linked glycoproteins	1188:1213	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	0	136	theme	Human	63:67	arg1	Serum					69:73	Human Serum	63:73	Human Serum using HILIC Enrichment and Spectral Library Search	63:124	Large-scale Identification of N-linked Intact Glycopeptides in Human Serum using HILIC Enrichment and Spectral Library Search.					
32102970	8	137	gly	deglycopeptides	1443:1457	arg2	deglycopeptides					1443:1457	these N-linked deglycopeptides	1428:1457	these N-linked deglycopeptides	1428:1457	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	5	138	theme	N-linked	1070:1077	arg1	glycopeptides					1086:1098	N-linked intact glycopeptides	1070:1098	N-linked intact glycopeptides	1070:1098	Both N-linked intact glycopeptides and deglycopeptides were analyzed by LC-MS/MS.					
32102970	9	139	contain	containing	1587:1596	arg1	database					1578:1585	A database	1576:1585	A database containing 739 N-glycan masses	1576:1616	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	9	139	contain	containing	1587:1596	arg2	masses					1611:1616	739 N-glycan masses	1598:1616	739 N-glycan masses	1598:1616	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	6	140	theme	N-linked	1192:1199	arg1	glycoproteins					1201:1213	764 N-linked glycoproteins	1188:1213	764 N-linked glycoproteins	1188:1213	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	4	141	theme	N-linked	1039:1046	arg1	deglycopeptides					1048:1062	N-linked deglycopeptides	1039:1062	N-linked deglycopeptides	1039:1062	After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides.					
32102970	6	142	link	N-linked	1152:1159	arg1	sets					1182:1185	N-linked deglycopeptides data sets	1152:1185	N-linked deglycopeptides data sets	1152:1185	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	8	143	theme	deglycopeptides	1486:1500	arg1	construction					1510:1521	N-linked deglycopeptides library construction	1477:1521	N-linked deglycopeptides library construction	1477:1521	The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides.					
32102970	1	144	theme	protein	334:340	arg1	abundances					342:351	serum protein abundances	328:351	serum protein abundances	328:351	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
32102970	3	145	theme	Serum	670:674	arg1	proteins					676:683	Serum proteins	670:683	Serum proteins	670:683	Serum proteins were firstly separated into low-abundance and high-abundance proteins by acetonitrile (ACN) precipitation.					
32102970	9	146	link	N-linked	1704:1711	arg1	glycopeptides					1720:1732	N-linked intact glycopeptides	1704:1732	N-linked intact glycopeptides	1704:1732	A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides.					
32102970	6	147	theme	data	1177:1180	arg1	sets					1182:1185	N-linked deglycopeptides data sets	1152:1185	N-linked deglycopeptides data sets	1152:1185	From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified.					
32102970	3	148	theme	low-abundance	713:725	arg1	proteins					746:753	low-abundance and high-abundance proteins	713:753	low-abundance and high-abundance proteins	713:753	Serum proteins were firstly separated into low-abundance and high-abundance proteins by acetonitrile (ACN) precipitation.					
32102970	1	149	theme	human	264:268	arg1	serum					270:274	human serum	264:274	human serum	264:274	Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms.					
34101384	10	0	theme	Protocol	1833:1840	arg1	variants					1808:1815	glycoform variants	1798:1815	glycoform variants of fetuin Basic Protocol 2	1798:1842	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	3	1	theme	glycans	534:540	arg1	analysis					522:529	the rapid, label-free analysis	500:529	the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor	500:696	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	1	2	used	used	270:273	arg2	class					180:184	a promising class	168:184	a promising class	168:184	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	1	2	used	used	270:273	arg2	Glycans					105:111	Glycans	105:111	Glycans (oligosaccharide chains attached to glycoproteins)	105:162	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	10	3	theme	biosensing	1756:1765	arg1	Protocol					1775:1782	glycan biosensing Support Protocol	1749:1782	glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS)	1749:1899	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	10	4	dep	Protocol	1699:1706	arg1	Preparing					1711:1719	Preparing	1711:1719	Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures	1711:1937	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	6	5	gly	glycoprotein	1016:1027	arg1	glycoprotein					1016:1027	glycoprotein samples	1016:1035	glycoprotein samples	1016:1035	Protocols for the preparation of glycoprotein samples and glycosylation analysis using NanoMonitor and lectin-based ELISA are described here.					
34101384	10	6	theme	Electrochemical	1856:1870	arg1	Spectroscopy					1882:1893	Performing Electrochemical Impedance Spectroscopy	1845:1893	glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS)	1749:1899	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	10	6	theme	Electrochemical	1856:1870	arg1	EIS					1896:1898	EIS	1896:1898	EIS	1896:1898	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	3	7	from	detail	479:484	arg1	article					467:473	this article	462:473	this article	462:473	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	3	8	theme	developed	561:569	arg1	platform					669:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform	548:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor	548:696	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	1	9	theme	body	210:213	arg1	fluids					215:220	body fluids	210:220	body fluids such as serum, saliva, urine, etc.	210:255	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	1	9	theme	body	210:213	arg1	serum					230:234	serum	230:234	serum	230:234	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	6	10	theme	glycoprotein	1016:1027	arg1	samples					1029:1035	glycoprotein samples	1016:1035	glycoprotein samples	1016:1035	Protocols for the preparation of glycoprotein samples and glycosylation analysis using NanoMonitor and lectin-based ELISA are described here.					
34101384	10	11	theme	glycoprotein	1915:1926	arg1	structures					1928:1937	glycoprotein structures	1915:1937	glycoprotein structures	1915:1937	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	1	12	gly	glycoproteins	149:161	arg1	glycoproteins					149:161	glycoproteins	149:161	glycoproteins	149:161	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	1	13	located	found	201:205	arg2	class					180:184	a promising class	168:184	a promising class	168:184	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	1	13	located	found	201:205	arg1	serum					230:234	serum	230:234	serum	230:234	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	1	13	located	found	201:205	arg1	fluids					215:220	body fluids	210:220	body fluids such as serum, saliva, urine, etc.	210:255	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	1	13	located	found	201:205	arg2	Glycans					105:111	Glycans	105:111	Glycans (oligosaccharide chains attached to glycoproteins)	105:162	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	5	14	theme	glycan-lectin	862:874	arg1	activity					884:891	This glycan-lectin binding activity	857:891	This glycan-lectin binding activity	857:891	This glycan-lectin binding activity modulates the impedance of the electrical double layer at the buffer-electrode interface.					
34101384	10	15	theme	glycan	1749:1754	arg1	Protocol					1775:1782	glycan biosensing Support Protocol	1749:1782	glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS)	1749:1899	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	3	16	theme	selective	592:600	arg1	platform					669:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform	548:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor	548:696	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	8	17	theme	handheld	1548:1555	arg1	device					1578:1583	a handheld electronic biosensor device	1546:1583	a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples	1546:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	7	18	attach	derived	1288:1294	arg1	cells					1334:1338	cultured human pancreatic cancer cells	1301:1338	cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1301:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	7	18	attach	derived	1288:1294	arg2	glycoproteins					1274:1286	glycoproteins	1274:1286	glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1274:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	1	19	dep	Glycans	105:111	arg1	chains					130:135	oligosaccharide chains	114:135	oligosaccharide chains attached to glycoproteins	114:161	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	3	20	theme	sensitive	578:586	arg1	platform					669:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform	548:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor	548:696	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	8	21	theme	biosensor	1568:1576	arg1	device					1578:1583	a handheld electronic biosensor device	1546:1583	a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples	1546:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	4	22	theme	affinity	751:758	arg1	capture					760:766	the specific affinity capture	738:766	the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins	738:854	The glycosensor operation is based on the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins.					
34101384	3	23	theme	biosensing	647:656	arg1	platform					669:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform	548:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor	548:696	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	6	24	theme	lectin-based	1086:1097	arg1	ELISA					1099:1103	lectin-based ELISA	1086:1103	lectin-based ELISA	1086:1103	Protocols for the preparation of glycoprotein samples and glycosylation analysis using NanoMonitor and lectin-based ELISA are described here.					
34101384	10	25	theme	glycoform	1798:1806	arg1	variants					1808:1815	glycoform variants	1798:1815	glycoform variants of fetuin Basic Protocol 2	1798:1842	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	7	26	theme	cancer	1327:1332	arg1	cells					1334:1338	cultured human pancreatic cancer cells	1301:1338	cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1301:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	1	27	theme	conditions	304:313	arg1	diagnosis					283:291	the diagnosis	279:291	the diagnosis of disease conditions	279:313	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	3	28	theme	impedance	618:626	arg1	platform					669:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform	548:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor	548:696	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	5	29	theme	electrical	924:933	arg1	layer					942:946	the electrical double layer	920:946	the electrical double layer	920:946	This glycan-lectin binding activity modulates the impedance of the electrical double layer at the buffer-electrode interface.					
34101384	8	30	theme	multiplexed	1597:1607	arg1	detection					1609:1617	routine multiplexed detection	1589:1617	routine multiplexed detection of glycan biomarkers from clinical samples	1589:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	7	31	theme	human	1310:1314	arg1	cells					1334:1338	cultured human pancreatic cancer cells	1301:1338	cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1301:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	1	32	theme	biomarkers	189:198	arg1	Glycans					105:111	Glycans	105:111	Glycans (oligosaccharide chains attached to glycoproteins)	105:162	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	1	32	theme	biomarkers	189:198	arg1	class					180:184	a promising class	168:184	a promising class	168:184	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	6	33	theme	analysis	1055:1062	arg1	preparation					1001:1011	the preparation	997:1011	the preparation of glycoprotein samples and glycosylation analysis	997:1062	Protocols for the preparation of glycoprotein samples and glycosylation analysis using NanoMonitor and lectin-based ELISA are described here.					
34101384	7	34	dep	glycoprotein	1250:1261	arg1	fetuin					1263:1268	fetuin	1263:1268	fetuin	1263:1268	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	8	35	theme	biomarkers	1629:1638	arg1	detection					1609:1617	routine multiplexed detection	1589:1617	routine multiplexed detection of glycan biomarkers from clinical samples	1589:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	4	36	theme	sensor	797:802	arg1	surface					804:810	the sensor surface	793:810	the sensor surface	793:810	The glycosensor operation is based on the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins.					
34101384	7	37	theme	glycoproteins	1274:1286	arg1	variants					1234:1241	glycoform variants	1224:1241	glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1224:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	8	38	from	samples	1654:1660	arg1	detection					1609:1617	routine multiplexed detection	1589:1617	routine multiplexed detection of glycan biomarkers from clinical samples	1589:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	8	38	from	samples	1654:1660	arg1	biomarkers					1629:1638	glycan biomarkers	1622:1638	glycan biomarkers from clinical samples	1622:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	8	39	theme	clinical	1645:1652	arg1	samples					1654:1660	clinical samples	1645:1660	clinical samples	1645:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	8	40	theme	NanoMonitor	1467:1477	arg1	protocols					1479:1487	NanoMonitor protocols	1467:1487	NanoMonitor protocols	1467:1487	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	2	41	theme	glycosylation	365:377	arg1	machinery					379:387	altered glycosylation machinery	357:387	altered glycosylation machinery	357:387	Subtle changes in glycans resulting from altered glycosylation machinery have been reported during various diseases, including carcinogenesis.					
34101384	0	42	theme	Label-Free	0:9	arg1	Analysis					33:40	Label-Free Protein Glycosylation Analysis	0:40	Label-Free Protein Glycosylation Analysis	0:40	Label-Free Protein Glycosylation Analysis Using NanoMonitor-An Ultrasensitive Electrochemical Biosensor.					
34101384	4	43	theme	target	775:780	arg1	glycans					782:788	the target glycans	771:788	the target glycans on the sensor surface	771:810	The glycosensor operation is based on the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins.					
34101384	2	44	theme	various	415:421	arg1	diseases					423:430	various diseases	415:430	various diseases	415:430	Subtle changes in glycans resulting from altered glycosylation machinery have been reported during various diseases, including carcinogenesis.					
34101384	2	44	theme	various	415:421	arg1	carcinogenesis					443:456	carcinogenesis	443:456	carcinogenesis	443:456	Subtle changes in glycans resulting from altered glycosylation machinery have been reported during various diseases, including carcinogenesis.					
34101384	0	45	theme	Glycosylation	19:31	arg1	Analysis					33:40	Label-Free Protein Glycosylation Analysis	0:40	Label-Free Protein Glycosylation Analysis	0:40	Label-Free Protein Glycosylation Analysis Using NanoMonitor-An Ultrasensitive Electrochemical Biosensor.					
34101384	7	46	dep	sensitivity	1350:1360	arg1	orders					1363:1368	orders	1363:1368	orders of magnitude higher than lectin-based ELISA	1363:1412	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	10	47	theme	Basic	1827:1831	arg1	Protocol					1833:1840	fetuin Basic Protocol 2	1820:1842	fetuin Basic Protocol 2	1820:1842	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	0	48	theme	Ultrasensitive	63:76	arg1	Biosensor					94:102	NanoMonitor-An Ultrasensitive Electrochemical Biosensor	48:102	NanoMonitor-An Ultrasensitive Electrochemical Biosensor	48:102	Label-Free Protein Glycosylation Analysis Using NanoMonitor-An Ultrasensitive Electrochemical Biosensor.					
34101384	7	49	theme	magnitude	1373:1381	arg1	orders					1363:1368	orders	1363:1368	orders of magnitude higher than lectin-based ELISA	1363:1412	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	8	50	theme	NanoMonitor	1531:1541	arg1	use					1524:1526	use	1524:1526	use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples	1524:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	3	51	theme	rapid	504:508	arg1	analysis					522:529	the rapid, label-free analysis	500:529	the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor	500:696	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	7	52	with	cells	1334:1338	arg1	sensitivity					1350:1360	high sensitivity	1345:1360	high sensitivity (orders of magnitude higher than lectin-based ELISA)	1345:1413	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	7	52	with	cells	1334:1338	arg1	selectivity					1419:1429	selectivity	1419:1429	selectivity	1419:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	10	53	theme	Basic	1693:1697	arg1	Protocol					1699:1706	Basic Protocol 1	1693:1708	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.	1693:1938	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	10	54	gly	glycoprotein	1915:1926	arg1	glycoprotein					1915:1926	glycoprotein structures	1915:1937	glycoprotein structures	1915:1937	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	2	55	from	changes	323:329	arg1	glycans					334:340	glycans	334:340	glycans resulting from altered glycosylation machinery	334:387	Subtle changes in glycans resulting from altered glycosylation machinery have been reported during various diseases, including carcinogenesis.					
34101384	10	56	theme	Impedance	1872:1880	arg1	Spectroscopy					1882:1893	Performing Electrochemical Impedance Spectroscopy	1845:1893	glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS)	1749:1899	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	10	56	theme	Impedance	1872:1880	arg1	EIS					1896:1898	EIS	1896:1898	EIS	1896:1898	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	6	57	theme	samples	1029:1035	arg1	preparation					1001:1011	the preparation	997:1011	the preparation of glycoprotein samples and glycosylation analysis	997:1062	Protocols for the preparation of glycoprotein samples and glycosylation analysis using NanoMonitor and lectin-based ELISA are described here.					
34101384	5	58	theme	layer	942:946	arg1	impedance					907:915	the impedance	903:915	the impedance of the electrical double layer	903:946	This glycan-lectin binding activity modulates the impedance of the electrical double layer at the buffer-electrode interface.					
34101384	10	59	theme	fetuin	1820:1825	arg1	Protocol					1833:1840	fetuin Basic Protocol 2	1820:1842	fetuin Basic Protocol 2	1820:1842	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	5	60	theme	binding	876:882	arg1	activity					884:891	This glycan-lectin binding activity	857:891	This glycan-lectin binding activity	857:891	This glycan-lectin binding activity modulates the impedance of the electrical double layer at the buffer-electrode interface.					
34101384	8	61	theme	electronic	1557:1566	arg1	device					1578:1583	a handheld electronic biosensor device	1546:1583	a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples	1546:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	10	62	theme	NanoMonitor	1725:1735	arg1	surface					1737:1743	the NanoMonitor surface	1721:1743	the NanoMonitor surface	1721:1743	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	1	63	attach	attached	137:144	arg2	chains					130:135	oligosaccharide chains	114:135	oligosaccharide chains attached to glycoproteins	114:161	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	1	63	attach	attached	137:144	arg1	glycoproteins					149:161	glycoproteins	149:161	glycoproteins	149:161	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	3	64	theme	spectroscopy-based	628:645	arg1	platform					669:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform	548:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor	548:696	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	0	65	theme	NanoMonitor-An	48:61	arg1	Biosensor					94:102	NanoMonitor-An Ultrasensitive Electrochemical Biosensor	48:102	NanoMonitor-An Ultrasensitive Electrochemical Biosensor	48:102	Label-Free Protein Glycosylation Analysis Using NanoMonitor-An Ultrasensitive Electrochemical Biosensor.					
34101384	8	66	theme	routine	1589:1595	arg1	detection					1609:1617	routine multiplexed detection	1589:1617	routine multiplexed detection of glycan biomarkers from clinical samples	1589:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	4	67	theme	specific	742:749	arg1	capture					760:766	the specific affinity capture	738:766	the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins	738:854	The glycosensor operation is based on the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins.					
34101384	7	68	theme	pancreatic	1316:1325	arg1	cells					1334:1338	cultured human pancreatic cancer cells	1301:1338	cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1301:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	3	69	theme	electrochemical	602:616	arg1	platform					669:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform	548:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor	548:696	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	1	70	theme	promising	170:178	arg1	Glycans					105:111	Glycans	105:111	Glycans (oligosaccharide chains attached to glycoproteins)	105:162	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	1	70	theme	promising	170:178	arg1	class					180:184	a promising class	168:184	a promising class	168:184	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	4	71	theme	glycosensor	704:714	arg1	operation					716:724	The glycosensor operation	700:724	The glycosensor operation	700:724	The glycosensor operation is based on the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins.					
34101384	7	72	theme	cultured	1301:1308	arg1	cells					1334:1338	cultured human pancreatic cancer cells	1301:1338	cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1301:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	4	73	from	surface	804:810	arg1	capture					760:766	the specific affinity capture	738:766	the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins	738:854	The glycosensor operation is based on the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins.					
34101384	5	74	theme	double	935:940	arg1	layer					942:946	the electrical double layer	920:946	the electrical double layer	920:946	This glycan-lectin binding activity modulates the impedance of the electrical double layer at the buffer-electrode interface.					
34101384	8	75	theme	glycan	1622:1627	arg1	biomarkers					1629:1638	glycan biomarkers	1622:1638	glycan biomarkers from clinical samples	1622:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	9	76	dep	LLC	1688:1690	arg1	Periodicals					1676:1686	Periodicals	1676:1686	Periodicals	1676:1686	© 2021 Wiley Periodicals LLC.					
34101384	6	77	theme	glycosylation	1041:1053	arg1	analysis					1055:1062	glycosylation analysis	1041:1062	glycosylation analysis	1041:1062	Protocols for the preparation of glycoprotein samples and glycosylation analysis using NanoMonitor and lectin-based ELISA are described here.					
34101384	4	78	theme	glycans	782:788	arg1	capture					760:766	the specific affinity capture	738:766	the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins	738:854	The glycosensor operation is based on the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins.					
34101384	4	79	from	glycans	782:788	arg1	surface					804:810	the sensor surface	793:810	the sensor surface	793:810	The glycosensor operation is based on the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins.					
34101384	5	80	theme	buffer-electrode	955:970	arg1	interface					972:980	the buffer-electrode interface	951:980	the buffer-electrode interface	951:980	This glycan-lectin binding activity modulates the impedance of the electrical double layer at the buffer-electrode interface.					
34101384	10	81	theme	Support	1767:1773	arg1	Protocol					1775:1782	glycan biosensing Support Protocol	1749:1782	glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS)	1749:1899	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	0	82	theme	Protein	11:17	arg1	Analysis					33:40	Label-Free Protein Glycosylation Analysis	0:40	Label-Free Protein Glycosylation Analysis	0:40	Label-Free Protein Glycosylation Analysis Using NanoMonitor-An Ultrasensitive Electrochemical Biosensor.					
34101384	1	83	theme	oligosaccharide	114:128	arg1	chains					130:135	oligosaccharide chains	114:135	oligosaccharide chains attached to glycoproteins	114:161	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	2	84	theme	altered	357:363	arg1	machinery					379:387	altered glycosylation machinery	357:387	altered glycosylation machinery	357:387	Subtle changes in glycans resulting from altered glycosylation machinery have been reported during various diseases, including carcinogenesis.					
34101384	8	85	from	detection	1609:1617	arg1	samples					1654:1660	clinical samples	1645:1660	clinical samples	1645:1660	The results obtained indicate that NanoMonitor protocols can be further developed to enable use of NanoMonitor as a handheld electronic biosensor device for routine multiplexed detection of glycan biomarkers from clinical samples.					
34101384	10	86	dep	Protocol	1775:1782	arg1	Spectroscopy					1882:1893	Performing Electrochemical Impedance Spectroscopy	1845:1893	glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS)	1749:1899	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	10	86	dep	Protocol	1775:1782	arg1	Synthesis					1785:1793	Synthesis	1785:1793	glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS)	1749:1899	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	10	86	dep	Protocol	1775:1782	arg1	EIS					1896:1898	EIS	1896:1898	EIS	1896:1898	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	4	87	from	capture	760:766	arg1	surface					804:810	the sensor surface	793:810	the sensor surface	793:810	The glycosensor operation is based on the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins.					
34101384	3	88	dep	rapid	504:508	arg1	label-free					511:520	label-free	511:520	label-free	511:520	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	7	89	theme	lectin-based	1395:1406	arg1	ELISA					1408:1412	lectin-based ELISA	1395:1412	lectin-based ELISA	1395:1412	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	7	90	gly	glycoproteins	1274:1286	arg1	glycoproteins					1274:1286	glycoproteins	1274:1286	glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1274:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	10	91	theme	Performing	1845:1854	arg1	Spectroscopy					1882:1893	Performing Electrochemical Impedance Spectroscopy	1845:1893	glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS)	1749:1899	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	10	91	theme	Performing	1845:1854	arg1	EIS					1896:1898	EIS	1896:1898	EIS	1896:1898	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	7	92	theme	glycoform	1224:1232	arg1	variants					1234:1241	glycoform variants	1224:1241	glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1224:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	7	93	theme	higher	1383:1388	arg1	orders					1363:1368	orders	1363:1368	orders of magnitude higher than lectin-based ELISA	1363:1412	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	3	94	theme	diagnostic	658:667	arg1	platform					669:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform	548:676	a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor	548:696	In this article, we detail protocols for the rapid, label-free analysis of glycans using a previously developed highly sensitive and selective electrochemical impedance spectroscopy-based biosensing diagnostic platform called "NanoMonitor."					
34101384	0	95	theme	Electrochemical	78:92	arg1	Biosensor					94:102	NanoMonitor-An Ultrasensitive Electrochemical Biosensor	48:102	NanoMonitor-An Ultrasensitive Electrochemical Biosensor	48:102	Label-Free Protein Glycosylation Analysis Using NanoMonitor-An Ultrasensitive Electrochemical Biosensor.					
34101384	2	96	theme	Subtle	316:321	arg1	changes					323:329	Subtle changes	316:329	Subtle changes in glycans resulting from altered glycosylation machinery	316:387	Subtle changes in glycans resulting from altered glycosylation machinery have been reported during various diseases, including carcinogenesis.					
34101384	1	97	theme	disease	296:302	arg1	conditions					304:313	disease conditions	296:313	disease conditions	296:313	Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions.					
34101384	7	98	gly	glycoprotein	1250:1261	arg1	glycoprotein					1250:1261	the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1246:1429	glycoprotein	1250:1261	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	10	99	theme	variants	1808:1815	arg1	Synthesis					1785:1793	Synthesis	1785:1793	glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS)	1749:1899	Basic Protocol 1: Preparing the NanoMonitor surface for glycan biosensing Support Protocol: Synthesis of glycoform variants of fetuin Basic Protocol 2: Performing Electrochemical Impedance Spectroscopy (EIS) for analyzing glycoprotein structures.					
34101384	7	100	theme	high	1345:1348	arg1	sensitivity					1350:1360	high sensitivity	1345:1360	high sensitivity (orders of magnitude higher than lectin-based ELISA)	1345:1413	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
34101384	4	101	theme	glycan-binding	815:828	arg1	proteins					830:837	glycan-binding proteins	815:837	glycan-binding proteins known as lectins	815:854	The glycosensor operation is based on the specific affinity capture of the target glycans on the sensor surface by glycan-binding proteins known as lectins.					
34101384	7	102	theme	glycoprotein	1250:1261	arg1	variants					1234:1241	glycoform variants	1224:1241	glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity	1224:1429	The data obtained using these protocols show that NanoMonitor is capable of distinguishing between glycoform variants of the glycoprotein fetuin and glycoproteins derived from cultured human pancreatic cancer cells with high sensitivity (orders of magnitude higher than lectin-based ELISA) and selectivity.					
32252422	0	0	theme	Dynamics	113:120	arg1	Data					122:125	Molecular Dynamics Data	103:125	Molecular Dynamics Data	103:125	Efficient Construction of Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data.					
32252422	1	1	theme	diverse	179:185	arg1	polymers					226:233	structurally diverse, conformationally complex carbohydrate polymers	166:233	structurally diverse, conformationally complex carbohydrate polymers	166:233	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	1	1	theme	diverse	179:185	arg1	Glycosaminoglycans					128:145	Glycosaminoglycans	128:145	Glycosaminoglycans (GAGs)	128:152	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	4	2	theme	GAG	646:648	arg1	flexibility					659:669	GAG backbone flexibility	646:669	GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles)	646:724	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	0	3	theme	Molecular	103:111	arg1	Data					122:125	Molecular Dynamics Data	103:125	Molecular Dynamics Data	103:125	Efficient Construction of Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data.					
32252422	7	4	theme	Non-sulfated	1238:1249	arg1	ensembles					1280:1288	Non-sulfated chondroitin 100- and 200-mer ensembles	1238:1288	Non-sulfated chondroitin 100- and 200-mer ensembles	1238:1288	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	4	5	theme	unbiased	731:738	arg1	simulations					769:779	unbiased all-atom explicit-solvent MD simulations	731:779	unbiased all-atom explicit-solvent MD simulations of short GAG polymers	731:801	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	6	6	contain	have	1092:1095	arg1	distributions					1036:1048	End-to-end distance distributions	1016:1048	End-to-end distance distributions in constructed and MD-generated ensembles	1016:1090	End-to-end distance distributions in constructed and MD-generated ensembles have minimal differences, suggesting that our algorithm produces conformational ensembles that mimic the backbone flexibility seen in simulation.					
32252422	6	6	contain	have	1092:1095	arg2	differences					1105:1115	minimal differences	1097:1115	minimal differences	1097:1115	End-to-end distance distributions in constructed and MD-generated ensembles have minimal differences, suggesting that our algorithm produces conformational ensembles that mimic the backbone flexibility seen in simulation.					
32252422	5	7	theme	internal	995:1002	arg1	validation					1004:1013	internal validation	995:1013	internal validation	995:1013	The algorithm was used to generate non-sulfated chondroitin 10- and 20-mer ensembles which were compared to MD-generated ensembles for internal validation.					
32252422	3	8	theme	Molecular	426:434	arg1	MD					446:447	MD	446:447	MD	446:447	Molecular dynamics (MD) simulations can overcome this challenge but are only feasible for short GAG polymers.					
32252422	3	8	theme	Molecular	426:434	arg1	dynamics					436:443	Molecular dynamics	426:443	Molecular dynamics (MD) simulations	426:460	Molecular dynamics (MD) simulations can overcome this challenge but are only feasible for short GAG polymers.					
32252422	2	9	theme	GAG	334:336	arg1	thermodynamics					353:366	GAG conformational thermodynamics	334:366	GAG conformational thermodynamics	334:366	These characteristics present a challenge for studying GAG conformational thermodynamics at atomic resolution using existing experimental methods.					
32252422	4	10	theme	polymers	794:801	arg1	simulations					769:779	unbiased all-atom explicit-solvent MD simulations	731:779	unbiased all-atom explicit-solvent MD simulations of short GAG polymers	731:801	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	6	11	theme	MD-generated	1069:1080	arg1	ensembles					1082:1090	MD-generated ensembles	1069:1090	MD-generated ensembles	1069:1090	End-to-end distance distributions in constructed and MD-generated ensembles have minimal differences, suggesting that our algorithm produces conformational ensembles that mimic the backbone flexibility seen in simulation.					
32252422	2	12	theme	atomic	371:376	arg1	resolution					378:387	atomic resolution	371:387	atomic resolution using existing experimental methods	371:423	These characteristics present a challenge for studying GAG conformational thermodynamics at atomic resolution using existing experimental methods.					
32252422	7	13	from	reduction	1371:1379	arg1	time					1384:1387	time	1384:1387	time	1384:1387	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	7	13	from	reduction	1371:1379	arg1	cost					1407:1410	computational cost	1393:1410	computational cost	1393:1410	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	4	14	theme	all-atom	740:747	arg1	simulations					769:779	unbiased all-atom explicit-solvent MD simulations	731:779	unbiased all-atom explicit-solvent MD simulations of short GAG polymers	731:801	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	4	15	theme	backbone	650:657	arg1	flexibility					659:669	GAG backbone flexibility	646:669	GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles)	646:724	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	4	16	theme	MD	766:767	arg1	simulations					769:779	unbiased all-atom explicit-solvent MD simulations	731:779	unbiased all-atom explicit-solvent MD simulations of short GAG polymers	731:801	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	6	17	theme	backbone	1197:1204	arg1	flexibility					1206:1216	the backbone flexibility	1193:1216	the backbone flexibility seen in simulation	1193:1235	End-to-end distance distributions in constructed and MD-generated ensembles have minimal differences, suggesting that our algorithm produces conformational ensembles that mimic the backbone flexibility seen in simulation.					
32252422	4	18	theme	GAG	790:792	arg1	polymers					794:801	short GAG polymers	784:801	short GAG polymers	784:801	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	7	19	from	efficiency	1339:1348	arg1	time					1384:1387	time	1384:1387	time	1384:1387	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	7	19	from	efficiency	1339:1348	arg1	cost					1407:1410	computational cost	1393:1410	computational cost	1393:1410	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	7	20	theme	100-	1263:1266	arg1	ensembles					1280:1288	Non-sulfated chondroitin 100- and 200-mer ensembles	1238:1288	Non-sulfated chondroitin 100- and 200-mer ensembles	1238:1288	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	5	21	used	used	878:881	arg2	algorithm					864:872	The algorithm	860:872	The algorithm	860:872	The algorithm was used to generate non-sulfated chondroitin 10- and 20-mer ensembles which were compared to MD-generated ensembles for internal validation.					
32252422	6	22	theme	distance	1027:1034	arg1	distributions					1036:1048	End-to-end distance distributions	1016:1048	End-to-end distance distributions in constructed and MD-generated ensembles	1016:1090	End-to-end distance distributions in constructed and MD-generated ensembles have minimal differences, suggesting that our algorithm produces conformational ensembles that mimic the backbone flexibility seen in simulation.					
32252422	1	23	dep	diverse	179:185	arg1	complex					205:211	complex	205:211	complex	205:211	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	1	24	theme	carbohydrate	213:224	arg1	polymers					226:233	structurally diverse, conformationally complex carbohydrate polymers	166:233	structurally diverse, conformationally complex carbohydrate polymers	166:233	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	1	24	theme	carbohydrate	213:224	arg1	Glycosaminoglycans					128:145	Glycosaminoglycans	128:145	Glycosaminoglycans (GAGs)	128:152	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	0	25	theme	Efficient	0:8	arg1	Construction					10:21	Efficient Construction	0:21	Efficient Construction of Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data.	0:126	Efficient Construction of Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data.					
32252422	4	26	theme	conformational	604:617	arg1	parameters					619:628	all conformational parameters	600:628	all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers	600:801	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	4	27	theme	short	784:788	arg1	polymers					794:801	short GAG polymers	784:801	short GAG polymers	784:801	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	4	28	theme	dihedral	709:716	arg1	angles					718:723	dihedral angles	709:723	dihedral angles	709:723	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	0	29	theme	Atomic-Resolution	26:42	arg1	Models					44:49	Atomic-Resolution Models	26:49	Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data	26:125	Efficient Construction of Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data.					
32252422	6	30	from	distributions	1036:1048	arg1	ensembles					1082:1090	MD-generated ensembles	1069:1090	MD-generated ensembles	1069:1090	End-to-end distance distributions in constructed and MD-generated ensembles have minimal differences, suggesting that our algorithm produces conformational ensembles that mimic the backbone flexibility seen in simulation.					
32252422	6	30	from	distributions	1036:1048	arg1	constructed					1053:1063	constructed	1053:1063	constructed	1053:1063	End-to-end distance distributions in constructed and MD-generated ensembles have minimal differences, suggesting that our algorithm produces conformational ensembles that mimic the backbone flexibility seen in simulation.					
32252422	6	31	theme	minimal	1097:1103	arg1	differences					1105:1115	minimal differences	1097:1115	minimal differences	1097:1115	End-to-end distance distributions in constructed and MD-generated ensembles have minimal differences, suggesting that our algorithm produces conformational ensembles that mimic the backbone flexibility seen in simulation.					
32252422	3	32	theme	GAG	522:524	arg1	polymers					526:533	short GAG polymers	516:533	short GAG polymers	516:533	Molecular dynamics (MD) simulations can overcome this challenge but are only feasible for short GAG polymers.					
32252422	4	33	dep	flexibility	659:669	arg1	lengths					683:689	bond lengths	678:689	bond lengths	678:689	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	4	33	dep	flexibility	659:669	arg1	angles					697:702	bond angles	692:702	bond angles	692:702	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	4	33	dep	flexibility	659:669	arg1	angles					718:723	dihedral angles	709:723	dihedral angles	709:723	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	2	34	theme	conformational	338:351	arg1	thermodynamics					353:366	GAG conformational thermodynamics	334:366	GAG conformational thermodynamics	334:366	These characteristics present a challenge for studying GAG conformational thermodynamics at atomic resolution using existing experimental methods.					
32252422	4	35	theme	explicit-solvent	749:764	arg1	simulations					769:779	unbiased all-atom explicit-solvent MD simulations	731:779	unbiased all-atom explicit-solvent MD simulations of short GAG polymers	731:801	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	5	36	theme	20-mer	928:933	arg1	ensembles					935:943	20-mer ensembles	928:943	20-mer ensembles	928:943	The algorithm was used to generate non-sulfated chondroitin 10- and 20-mer ensembles which were compared to MD-generated ensembles for internal validation.					
32252422	7	37	theme	chondroitin	1251:1261	arg1	ensembles					1280:1288	Non-sulfated chondroitin 100- and 200-mer ensembles	1238:1288	Non-sulfated chondroitin 100- and 200-mer ensembles	1238:1288	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	0	38	theme	Models	44:49	arg1	Construction					10:21	Efficient Construction	0:21	Efficient Construction of Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data.	0:126	Efficient Construction of Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data.					
32252422	6	39	theme	End-to-end	1016:1025	arg1	distributions					1036:1048	End-to-end distance distributions	1016:1048	End-to-end distance distributions in constructed and MD-generated ensembles	1016:1090	End-to-end distance distributions in constructed and MD-generated ensembles have minimal differences, suggesting that our algorithm produces conformational ensembles that mimic the backbone flexibility seen in simulation.					
32252422	7	40	theme	200-mer	1272:1278	arg1	ensembles					1280:1288	Non-sulfated chondroitin 100- and 200-mer ensembles	1238:1288	Non-sulfated chondroitin 100- and 200-mer ensembles	1238:1288	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	5	41	theme	MD-generated	968:979	arg1	ensembles					981:989	MD-generated ensembles	968:989	MD-generated ensembles for internal validation	968:1013	The algorithm was used to generate non-sulfated chondroitin 10- and 20-mer ensembles which were compared to MD-generated ensembles for internal validation.					
32252422	5	42	theme	non-sulfated	895:906	arg1	chondroitin					908:918	non-sulfated chondroitin 10-	895:922	non-sulfated chondroitin 10-	895:922	The algorithm was used to generate non-sulfated chondroitin 10- and 20-mer ensembles which were compared to MD-generated ensembles for internal validation.					
32252422	3	43	theme	short	516:520	arg1	polymers					526:533	short GAG polymers	516:533	short GAG polymers	516:533	Molecular dynamics (MD) simulations can overcome this challenge but are only feasible for short GAG polymers.					
32252422	0	44	theme	Chondroitin	67:77	arg1	Glycosaminoglycan					79:95	Non-Sulfated Chondroitin Glycosaminoglycan	54:95	Non-Sulfated Chondroitin Glycosaminoglycan	54:95	Efficient Construction of Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data.					
32252422	1	45	dep	200	258:260	arg1	to					255:256	to	255:256	to	255:256	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	2	46	theme	experimental	404:415	arg1	methods					417:423	existing experimental methods	395:423	existing experimental methods	395:423	These characteristics present a challenge for studying GAG conformational thermodynamics at atomic resolution using existing experimental methods.					
32252422	0	47	theme	Non-Sulfated	54:65	arg1	Glycosaminoglycan					79:95	Non-Sulfated Chondroitin Glycosaminoglycan	54:95	Non-Sulfated Chondroitin Glycosaminoglycan	54:95	Efficient Construction of Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data.					
32252422	6	48	theme	conformational	1157:1170	arg1	ensembles					1172:1180	conformational ensembles	1157:1180	conformational ensembles that mimic the backbone flexibility seen in simulation	1157:1235	End-to-end distance distributions in constructed and MD-generated ensembles have minimal differences, suggesting that our algorithm produces conformational ensembles that mimic the backbone flexibility seen in simulation.					
32252422	4	49	dep	lengths	683:689	arg1	i.e.					672:675	i.e.	672:675	i.e.	672:675	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	7	50	theme	computational	1393:1405	arg1	cost					1407:1410	computational cost	1393:1410	computational cost	1393:1410	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	2	51	theme	existing	395:402	arg1	methods					417:423	existing experimental methods	395:423	existing experimental methods	395:423	These characteristics present a challenge for studying GAG conformational thermodynamics at atomic resolution using existing experimental methods.					
32252422	1	52	contain	contain	244:250	arg1	polymers					226:233	structurally diverse, conformationally complex carbohydrate polymers	166:233	structurally diverse, conformationally complex carbohydrate polymers	166:233	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	1	52	contain	contain	244:250	arg1	Glycosaminoglycans					128:145	Glycosaminoglycans	128:145	Glycosaminoglycans (GAGs)	128:152	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	1	52	contain	contain	244:250	arg2	monosaccharides					262:276	up to 200 monosaccharides	252:276	up to 200 monosaccharides	252:276	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	4	53	theme	GAGs	834:837	arg1	models					824:829	models	824:829	models of GAGs of arbitrary length	824:857	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	1	54	theme	linear	158:163	arg1	polymers					226:233	structurally diverse, conformationally complex carbohydrate polymers	166:233	structurally diverse, conformationally complex carbohydrate polymers	166:233	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	1	54	theme	linear	158:163	arg1	Glycosaminoglycans					128:145	Glycosaminoglycans	128:145	Glycosaminoglycans (GAGs)	128:152	Glycosaminoglycans (GAGs) are linear, structurally diverse, conformationally complex carbohydrate polymers that may contain up to 200 monosaccharides.					
32252422	4	55	theme	bond	692:695	arg1	angles					697:702	bond angles	692:702	bond angles	692:702	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	4	56	theme	length	852:857	arg1	GAGs					834:837	GAGs	834:837	GAGs of arbitrary length	834:857	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	4	57	theme	bond	678:681	arg1	lengths					683:689	bond lengths	678:689	bond lengths	678:689	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	0	58	theme	Glycosaminoglycan	79:95	arg1	Models					44:49	Atomic-Resolution Models	26:49	Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data	26:125	Efficient Construction of Atomic-Resolution Models of Non-Sulfated Chondroitin Glycosaminoglycan Using Molecular Dynamics Data.					
32252422	4	59	theme	arbitrary	842:850	arg1	length					852:857	arbitrary length	842:857	arbitrary length	842:857	To address this problem, we developed an algorithm that applies all conformational parameters contributing to GAG backbone flexibility (i.e., bond lengths, bond angles, and dihedral angles) from unbiased all-atom explicit-solvent MD simulations of short GAG polymers to rapidly construct models of GAGs of arbitrary length.					
32252422	7	60	theme	algorithm	1357:1365	arg1	reduction					1371:1379	reduction	1371:1379	reduction in time and computational cost compared to simulation	1371:1433	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	7	60	theme	algorithm	1357:1365	arg1	efficiency					1339:1348	the efficiency	1335:1348	the efficiency of the algorithm	1335:1365	Non-sulfated chondroitin 100- and 200-mer ensembles were constructed within a day, demonstrating the efficiency of the algorithm and reduction in time and computational cost compared to simulation.					
32252422	3	61	theme	dynamics	436:443	arg1	simulations					450:460	Molecular dynamics (MD) simulations	426:460	Molecular dynamics (MD) simulations	426:460	Molecular dynamics (MD) simulations can overcome this challenge but are only feasible for short GAG polymers.					
34067878	4	0	theme	glycan	511:516	arg1	types					518:522	distinctive glycan types	499:522	distinctive glycan types	499:522	Both the Spike and ACE2 are densely glycosylated, and it is unclear how distinctive glycan types may modulate the interaction of RBD and ACE2.					
34067878	9	1	theme	prevalent	1304:1312	arg1	mutation					1320:1327	the recently prevalent N501Y mutation	1291:1327	the recently prevalent N501Y mutation	1291:1327	Applying our simulated results, we illustrate how the recently prevalent N501Y mutation may alter specific interactions with host ACE2 that facilitate the virus-host binding.					
34067878	6	2	theme	MAN9	807:810	arg1	glycans					812:818	oligomannose MAN9 glycans	794:818	oligomannose MAN9 glycans	794:818	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	6	3	with	RBD-ACE2	839:846	arg1	glycans					812:818	oligomannose MAN9 glycans	794:818	oligomannose MAN9 glycans	794:818	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	6	3	with	RBD-ACE2	839:846	arg1	glycans					865:871	complex FA2 glycans	853:871	complex FA2 glycans	853:871	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	7	4	from	interface	942:950	arg1	residues					917:924	the key residues	909:924	the key residues at the RBD-ACE2 interface that form contacts with higher probabilities, thus providing a quantitative evaluation that complements recent structural studies	909:1080	These simulations identify the key residues at the RBD-ACE2 interface that form contacts with higher probabilities, thus providing a quantitative evaluation that complements recent structural studies.					
34067878	2	5	theme	new	256:258	arg1	challenges					260:269	new challenges	256:269	new challenges for vaccine design	256:288	It has become a global pandemic and its frequent mutations may pose new challenges for vaccine design.					
34067878	12	6	theme	effective	1858:1866	arg1	treatments					1868:1877	effective treatments	1858:1877	effective treatments that aim	1858:1886	Together, our results provide a more comprehensive picture of the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus.					
34067878	7	7	theme	higher	976:981	arg1	probabilities					983:995	higher probabilities	976:995	higher probabilities	976:995	These simulations identify the key residues at the RBD-ACE2 interface that form contacts with higher probabilities, thus providing a quantitative evaluation that complements recent structural studies.					
34067878	6	8	from	glycans	766:772	arg1	ACE2					876:879	ACE2	876:879	ACE2	876:879	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	6	8	from	glycans	766:772	arg1	ACE2					823:826	ACE2	823:826	ACE2	823:826	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	11	9	theme	enhanced	1664:1671	arg1	binding					1673:1679	enhanced binding	1664:1679	enhanced binding of the complex	1664:1694	Finally, an energetics analysis shows that MAN9 glycans on ACE2 decrease RBD-ACE2 affinity, while FA2 glycans lead to enhanced binding of the complex.					
34067878	6	10	theme	extensive	699:707	arg1	simulations					718:728	extensive all-atom simulations	699:728	extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2	699:879	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	1	11	theme	coronavirus	164:174	arg1	SARS-CoV-2					176:185	the novel coronavirus SARS-CoV-2	154:185	the novel coronavirus SARS-CoV-2	154:185	COVID-19 is a highly infectious respiratory disease caused by the novel coronavirus SARS-CoV-2.					
34067878	10	12	theme	ACE2	1479:1482	arg1	Asn90					1470:1474	Asn90	1470:1474	Asn90 of ACE2	1470:1482	Furthermore, our simulations reveal how the glycan on Asn90 of ACE2 can play a distinct role in the binding and unbinding of RBD.					
34067878	9	13	theme	host	1366:1369	arg1	ACE2					1371:1374	host ACE2	1366:1374	host ACE2	1366:1374	Applying our simulated results, we illustrate how the recently prevalent N501Y mutation may alter specific interactions with host ACE2 that facilitate the virus-host binding.					
34067878	11	14	theme	complex	1688:1694	arg1	binding					1673:1679	enhanced binding	1664:1679	enhanced binding of the complex	1664:1694	Finally, an energetics analysis shows that MAN9 glycans on ACE2 decrease RBD-ACE2 affinity, while FA2 glycans lead to enhanced binding of the complex.					
34067878	5	15	theme	novel	645:649	arg1	strategies					663:672	novel therapeutic strategies	645:672	novel therapeutic strategies	645:672	Detailed understanding of these determinants is key for the development of novel therapeutic strategies.					
34067878	2	16	theme	global	204:209	arg1	pandemic					211:218	a global pandemic	202:218	a global pandemic	202:218	It has become a global pandemic and its frequent mutations may pose new challenges for vaccine design.					
34067878	8	17	theme	glycoforms	1181:1190	arg1	presence					1159:1166	the presence	1155:1166	the presence of different glycoforms	1155:1190	Notably, we find that this RBD-ACE2 contact signature is not altered by the presence of different glycoforms, suggesting that RBD-ACE2 interaction is robust.					
34067878	8	18	theme	RBD-ACE2	1110:1117	arg1	signature					1127:1135	this RBD-ACE2 contact signature	1105:1135	this RBD-ACE2 contact signature	1105:1135	Notably, we find that this RBD-ACE2 contact signature is not altered by the presence of different glycoforms, suggesting that RBD-ACE2 interaction is robust.					
34067878	8	19	theme	different	1171:1179	arg1	glycoforms					1181:1190	different glycoforms	1171:1190	different glycoforms	1171:1190	Notably, we find that this RBD-ACE2 contact signature is not altered by the presence of different glycoforms, suggesting that RBD-ACE2 interaction is robust.					
34067878	10	20	theme	distinct	1495:1502	arg1	role					1504:1507	a distinct role	1493:1507	a distinct role	1493:1507	Furthermore, our simulations reveal how the glycan on Asn90 of ACE2 can play a distinct role in the binding and unbinding of RBD.					
34067878	11	21	theme	RBD-ACE2	1619:1626	arg1	affinity					1628:1635	RBD-ACE2 affinity	1619:1635	RBD-ACE2 affinity	1619:1635	Finally, an energetics analysis shows that MAN9 glycans on ACE2 decrease RBD-ACE2 affinity, while FA2 glycans lead to enhanced binding of the complex.					
34067878	7	22	theme	key	913:915	arg1	residues					917:924	the key residues	909:924	the key residues at the RBD-ACE2 interface that form contacts with higher probabilities, thus providing a quantitative evaluation that complements recent structural studies	909:1080	These simulations identify the key residues at the RBD-ACE2 interface that form contacts with higher probabilities, thus providing a quantitative evaluation that complements recent structural studies.					
34067878	2	23	theme	frequent	228:235	arg1	mutations					237:245	its frequent mutations	224:245	its frequent mutations	224:245	It has become a global pandemic and its frequent mutations may pose new challenges for vaccine design.					
34067878	1	24	theme	respiratory	124:134	arg1	COVID-19					92:99	COVID-19	92:99	COVID-19	92:99	COVID-19 is a highly infectious respiratory disease caused by the novel coronavirus SARS-CoV-2.					
34067878	1	24	theme	respiratory	124:134	arg1	disease					136:142	a highly infectious respiratory disease	104:142	a highly infectious respiratory disease caused by the novel coronavirus SARS-CoV-2	104:185	COVID-19 is a highly infectious respiratory disease caused by the novel coronavirus SARS-CoV-2.					
34067878	11	25	from	glycans	1594:1600	arg1	ACE2					1605:1608	ACE2	1605:1608	ACE2	1605:1608	Finally, an energetics analysis shows that MAN9 glycans on ACE2 decrease RBD-ACE2 affinity, while FA2 glycans lead to enhanced binding of the complex.					
34067878	0	26	from	Role	14:17	arg1	Interaction					37:47	the Interaction	33:47	the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2	33:89	Exploring the Role of Glycans in the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2.					
34067878	11	27	theme	FA2	1644:1646	arg1	glycans					1648:1654	FA2 glycans	1644:1654	FA2 glycans	1644:1654	Finally, an energetics analysis shows that MAN9 glycans on ACE2 decrease RBD-ACE2 affinity, while FA2 glycans lead to enhanced binding of the complex.					
34067878	0	28	theme	RBD	63:65	arg1	Interaction					37:47	the Interaction	33:47	the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2	33:89	Exploring the Role of Glycans in the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2.					
34067878	12	29	theme	interplay	1772:1780	arg1	picture					1748:1754	a more comprehensive picture	1727:1754	a more comprehensive picture of the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus	1727:1947	Together, our results provide a more comprehensive picture of the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus.					
34067878	0	30	theme	Human	71:75	arg1	ACE2					86:89	Human Receptor ACE2	71:89	Human Receptor ACE2	71:89	Exploring the Role of Glycans in the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2.					
34067878	6	31	theme	FA2	861:863	arg1	glycans					865:871	complex FA2 glycans	853:871	complex FA2 glycans	853:871	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	0	32	theme	ACE2	86:89	arg1	Interaction					37:47	the Interaction	33:47	the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2	33:89	Exploring the Role of Glycans in the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2.					
34067878	6	33	with	glycans	766:772	arg1	glycans					812:818	oligomannose MAN9 glycans	794:818	oligomannose MAN9 glycans	794:818	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	6	33	with	glycans	766:772	arg1	glycans					865:871	complex FA2 glycans	853:871	complex FA2 glycans	853:871	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	3	34	theme	SARS-CoV-2	332:341	arg1	RBD					325:327	the Spike RBD	315:327	the Spike RBD of SARS-CoV-2	315:341	During viral infection, the Spike RBD of SARS-CoV-2 binds the human host cell receptor ACE2, enabling the virus to enter the host cell.					
34067878	6	35	from	RBD-ACE2	839:846	arg1	ACE2					876:879	ACE2	876:879	ACE2	876:879	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	6	35	from	RBD-ACE2	839:846	arg1	ACE2					823:826	ACE2	823:826	ACE2	823:826	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	7	36	theme	recent	1056:1061	arg1	studies					1074:1080	recent structural studies	1056:1080	recent structural studies	1056:1080	These simulations identify the key residues at the RBD-ACE2 interface that form contacts with higher probabilities, thus providing a quantitative evaluation that complements recent structural studies.					
34067878	3	37	theme	receptor	369:376	arg1	ACE2					378:381	the human host cell receptor ACE2	349:381	the human host cell receptor ACE2	349:381	During viral infection, the Spike RBD of SARS-CoV-2 binds the human host cell receptor ACE2, enabling the virus to enter the host cell.					
34067878	0	38	theme	Glycans	22:28	arg1	Role					14:17	the Role	10:17	the Role of Glycans in the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2	10:89	Exploring the Role of Glycans in the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2.					
34067878	3	39	theme	host	359:362	arg1	ACE2					378:381	the human host cell receptor ACE2	349:381	the human host cell receptor ACE2	349:381	During viral infection, the Spike RBD of SARS-CoV-2 binds the human host cell receptor ACE2, enabling the virus to enter the host cell.					
34067878	11	40	theme	energetics	1558:1567	arg1	analysis					1569:1576	an energetics analysis	1555:1576	an energetics analysis	1555:1576	Finally, an energetics analysis shows that MAN9 glycans on ACE2 decrease RBD-ACE2 affinity, while FA2 glycans lead to enhanced binding of the complex.					
34067878	7	41	theme	quantitative	1015:1026	arg1	evaluation					1028:1037	a quantitative evaluation	1013:1037	a quantitative evaluation that complements recent structural studies	1013:1080	These simulations identify the key residues at the RBD-ACE2 interface that form contacts with higher probabilities, thus providing a quantitative evaluation that complements recent structural studies.					
34067878	7	42	with	contacts	962:969	arg1	probabilities					983:995	higher probabilities	976:995	higher probabilities	976:995	These simulations identify the key residues at the RBD-ACE2 interface that form contacts with higher probabilities, thus providing a quantitative evaluation that complements recent structural studies.					
34067878	6	43	theme	RBD-ACE2	741:748	arg1	complex					750:756	the (i) RBD-ACE2 complex	733:756	the (i) RBD-ACE2 complex	733:756	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	11	44	theme	MAN9	1589:1592	arg1	glycans					1594:1600	MAN9 glycans	1589:1600	MAN9 glycans on ACE2	1589:1608	Finally, an energetics analysis shows that MAN9 glycans on ACE2 decrease RBD-ACE2 affinity, while FA2 glycans lead to enhanced binding of the complex.					
34067878	12	45	theme	treatments	1868:1877	arg1	discovery					1845:1853	the discovery	1841:1853	the discovery of effective treatments that aim	1841:1886	Together, our results provide a more comprehensive picture of the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus.					
34067878	5	46	theme	Detailed	570:577	arg1	understanding					579:591	Detailed understanding	570:591	Detailed understanding of these determinants	570:613	Detailed understanding of these determinants is key for the development of novel therapeutic strategies.					
34067878	4	47	theme	distinctive	499:509	arg1	types					518:522	distinctive glycan types	499:522	distinctive glycan types	499:522	Both the Spike and ACE2 are densely glycosylated, and it is unclear how distinctive glycan types may modulate the interaction of RBD and ACE2.					
34067878	6	48	from	RBD-ACE2	780:787	arg1	ACE2					876:879	ACE2	876:879	ACE2	876:879	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	6	48	from	RBD-ACE2	780:787	arg1	ACE2					823:826	ACE2	823:826	ACE2	823:826	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	9	49	theme	N501Y	1314:1318	arg1	mutation					1320:1327	the recently prevalent N501Y mutation	1291:1327	the recently prevalent N501Y mutation	1291:1327	Applying our simulated results, we illustrate how the recently prevalent N501Y mutation may alter specific interactions with host ACE2 that facilitate the virus-host binding.					
34067878	6	50	theme	oligomannose	794:805	arg1	glycans					812:818	oligomannose MAN9 glycans	794:818	oligomannose MAN9 glycans	794:818	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	1	51	theme	novel	158:162	arg1	SARS-CoV-2					176:185	the novel coronavirus SARS-CoV-2	154:185	the novel coronavirus SARS-CoV-2	154:185	COVID-19 is a highly infectious respiratory disease caused by the novel coronavirus SARS-CoV-2.					
34067878	3	52	theme	cell	364:367	arg1	ACE2					378:381	the human host cell receptor ACE2	349:381	the human host cell receptor ACE2	349:381	During viral infection, the Spike RBD of SARS-CoV-2 binds the human host cell receptor ACE2, enabling the virus to enter the host cell.					
34067878	8	53	theme	contact	1119:1125	arg1	signature					1127:1135	this RBD-ACE2 contact signature	1105:1135	this RBD-ACE2 contact signature	1105:1135	Notably, we find that this RBD-ACE2 contact signature is not altered by the presence of different glycoforms, suggesting that RBD-ACE2 interaction is robust.					
34067878	6	54	theme	all-atom	709:716	arg1	simulations					718:728	extensive all-atom simulations	699:728	extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2	699:879	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	9	55	theme	specific	1339:1346	arg1	interactions					1348:1359	specific interactions	1339:1359	specific interactions with host ACE2 that facilitate the virus-host binding	1339:1413	Applying our simulated results, we illustrate how the recently prevalent N501Y mutation may alter specific interactions with host ACE2 that facilitate the virus-host binding.					
34067878	6	56	theme	complex	853:859	arg1	glycans					865:871	complex FA2 glycans	853:871	complex FA2 glycans	853:871	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	2	57	theme	vaccine	275:281	arg1	design					283:288	vaccine design	275:288	vaccine design	275:288	It has become a global pandemic and its frequent mutations may pose new challenges for vaccine design.					
34067878	12	58	theme	physical-chemical	1906:1922	arg1	properties					1924:1933	the physical-chemical properties	1902:1933	the physical-chemical properties of this virus	1902:1947	Together, our results provide a more comprehensive picture of the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus.					
34067878	4	59	theme	RBD	556:558	arg1	interaction					541:551	the interaction	537:551	the interaction of RBD and ACE2	537:567	Both the Spike and ACE2 are densely glycosylated, and it is unclear how distinctive glycan types may modulate the interaction of RBD and ACE2.					
34067878	12	60	theme	human	1800:1804	arg1	receptor					1806:1813	human receptor	1800:1813	human receptor	1800:1813	Together, our results provide a more comprehensive picture of the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus.					
34067878	4	61	theme	ACE2	564:567	arg1	interaction					541:551	the interaction	537:551	the interaction of RBD and ACE2	537:567	Both the Spike and ACE2 are densely glycosylated, and it is unclear how distinctive glycan types may modulate the interaction of RBD and ACE2.					
34067878	5	62	theme	therapeutic	651:661	arg1	strategies					663:672	novel therapeutic strategies	645:672	novel therapeutic strategies	645:672	Detailed understanding of these determinants is key for the development of novel therapeutic strategies.					
34067878	5	63	theme	determinants	602:613	arg1	understanding					579:591	Detailed understanding	570:591	Detailed understanding of these determinants	570:613	Detailed understanding of these determinants is key for the development of novel therapeutic strategies.					
34067878	6	64	dep	RBD-ACE2	839:846	arg1	iii					834:836	iii	834:836	iii	834:836	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	6	65	theme	complex	750:756	arg1	simulations					718:728	extensive all-atom simulations	699:728	extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2	699:879	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	9	66	theme	virus-host	1396:1405	arg1	binding					1407:1413	the virus-host binding	1392:1413	the virus-host binding	1392:1413	Applying our simulated results, we illustrate how the recently prevalent N501Y mutation may alter specific interactions with host ACE2 that facilitate the virus-host binding.					
34067878	12	67	theme	virus	1943:1947	arg1	properties					1924:1933	the physical-chemical properties	1902:1933	the physical-chemical properties of this virus	1902:1947	Together, our results provide a more comprehensive picture of the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus.					
34067878	0	68	theme	SARS-CoV-2	52:61	arg1	RBD					63:65	SARS-CoV-2 RBD	52:65	SARS-CoV-2 RBD	52:65	Exploring the Role of Glycans in the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2.					
34067878	4	69	gly	glycosylated	463:474	arg1	ACE2					446:449	ACE2	446:449	ACE2	446:449	Both the Spike and ACE2 are densely glycosylated, and it is unclear how distinctive glycan types may modulate the interaction of RBD and ACE2.					
34067878	4	69	gly	glycosylated	463:474	arg1	Spike					436:440	the Spike	432:440	the Spike	432:440	Both the Spike and ACE2 are densely glycosylated, and it is unclear how distinctive glycan types may modulate the interaction of RBD and ACE2.					
34067878	1	70	theme	infectious	113:122	arg1	COVID-19					92:99	COVID-19	92:99	COVID-19	92:99	COVID-19 is a highly infectious respiratory disease caused by the novel coronavirus SARS-CoV-2.					
34067878	1	70	theme	infectious	113:122	arg1	disease					136:142	a highly infectious respiratory disease	104:142	a highly infectious respiratory disease caused by the novel coronavirus SARS-CoV-2	104:185	COVID-19 is a highly infectious respiratory disease caused by the novel coronavirus SARS-CoV-2.					
34067878	5	71	theme	strategies	663:672	arg1	development					630:640	the development	626:640	the development of novel therapeutic strategies	626:672	Detailed understanding of these determinants is key for the development of novel therapeutic strategies.					
34067878	3	72	theme	viral	298:302	arg1	infection					304:312	viral infection	298:312	viral infection	298:312	During viral infection, the Spike RBD of SARS-CoV-2 binds the human host cell receptor ACE2, enabling the virus to enter the host cell.					
34067878	8	73	theme	RBD-ACE2	1209:1216	arg1	interaction					1218:1228	RBD-ACE2 interaction	1209:1228	RBD-ACE2 interaction	1209:1228	Notably, we find that this RBD-ACE2 contact signature is not altered by the presence of different glycoforms, suggesting that RBD-ACE2 interaction is robust.					
34067878	0	74	theme	Receptor	77:84	arg1	ACE2					86:89	Human Receptor ACE2	71:89	Human Receptor ACE2	71:89	Exploring the Role of Glycans in the Interaction of SARS-CoV-2 RBD and Human Receptor ACE2.					
34067878	3	75	theme	Spike	319:323	arg1	RBD					325:327	the Spike RBD	315:327	the Spike RBD of SARS-CoV-2	315:341	During viral infection, the Spike RBD of SARS-CoV-2 binds the human host cell receptor ACE2, enabling the virus to enter the host cell.					
34067878	7	76	theme	structural	1063:1072	arg1	studies					1074:1080	recent structural studies	1056:1080	recent structural studies	1056:1080	These simulations identify the key residues at the RBD-ACE2 interface that form contacts with higher probabilities, thus providing a quantitative evaluation that complements recent structural studies.					
34067878	12	77	theme	detailed	1763:1770	arg1	interplay					1772:1780	the detailed interplay	1759:1780	the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus	1759:1947	Together, our results provide a more comprehensive picture of the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus.					
34067878	3	78	theme	human	353:357	arg1	ACE2					378:381	the human host cell receptor ACE2	349:381	the human host cell receptor ACE2	349:381	During viral infection, the Spike RBD of SARS-CoV-2 binds the human host cell receptor ACE2, enabling the virus to enter the host cell.					
34067878	3	79	theme	host	416:419	arg1	cell					421:424	the host cell	412:424	the host cell	412:424	During viral infection, the Spike RBD of SARS-CoV-2 binds the human host cell receptor ACE2, enabling the virus to enter the host cell.					
34067878	12	80	theme	comprehensive	1734:1746	arg1	picture					1748:1754	a more comprehensive picture	1727:1754	a more comprehensive picture of the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus	1727:1947	Together, our results provide a more comprehensive picture of the detailed interplay between virus and human receptor, which is much needed for the discovery of effective treatments that aim at modulating the physical-chemical properties of this virus.					
34067878	10	81	theme	RBD	1541:1543	arg1	unbinding					1528:1536	unbinding	1528:1536	unbinding	1528:1536	Furthermore, our simulations reveal how the glycan on Asn90 of ACE2 can play a distinct role in the binding and unbinding of RBD.					
34067878	10	81	theme	RBD	1541:1543	arg1	binding					1516:1522	binding	1516:1522	binding	1516:1522	Furthermore, our simulations reveal how the glycan on Asn90 of ACE2 can play a distinct role in the binding and unbinding of RBD.					
34067878	9	82	theme	simulated	1254:1262	arg1	results					1264:1270	our simulated results	1250:1270	our simulated results	1250:1270	Applying our simulated results, we illustrate how the recently prevalent N501Y mutation may alter specific interactions with host ACE2 that facilitate the virus-host binding.					
34067878	7	83	theme	RBD-ACE2	933:940	arg1	interface					942:950	the RBD-ACE2 interface	929:950	the RBD-ACE2 interface	929:950	These simulations identify the key residues at the RBD-ACE2 interface that form contacts with higher probabilities, thus providing a quantitative evaluation that complements recent structural studies.					
34067878	9	84	with	interactions	1348:1359	arg1	ACE2					1371:1374	host ACE2	1366:1374	host ACE2	1366:1374	Applying our simulated results, we illustrate how the recently prevalent N501Y mutation may alter specific interactions with host ACE2 that facilitate the virus-host binding.					
34067878	10	85	from	glycan	1460:1465	arg1	Asn90					1470:1474	Asn90	1470:1474	Asn90 of ACE2	1470:1482	Furthermore, our simulations reveal how the glycan on Asn90 of ACE2 can play a distinct role in the binding and unbinding of RBD.					
34067878	6	86	dep	RBD-ACE2	780:787	arg1	ii					776:777	ii	776:777	ii	776:777	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	10	87	dep	binding	1516:1522	arg1	the					1512:1514	the	1512:1514	the	1512:1514	Furthermore, our simulations reveal how the glycan on Asn90 of ACE2 can play a distinct role in the binding and unbinding of RBD.					
34067878	6	88	with	RBD-ACE2	780:787	arg1	glycans					812:818	oligomannose MAN9 glycans	794:818	oligomannose MAN9 glycans	794:818	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34067878	6	88	with	RBD-ACE2	780:787	arg1	glycans					865:871	complex FA2 glycans	853:871	complex FA2 glycans	853:871	To this end, we perform extensive all-atom simulations of the (i) RBD-ACE2 complex without glycans, (ii) RBD-ACE2 with oligomannose MAN9 glycans in ACE2, and (iii) RBD-ACE2 with complex FA2 glycans in ACE2.					
34022314	6	0	with	MFP	1273:1275	arg1	proportions					1307:1317	different monosaccharide proportions	1282:1317	different monosaccharide proportions	1282:1317	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	6	1	theme	monosaccharide	1292:1305	arg1	proportions					1307:1317	different monosaccharide proportions	1282:1317	different monosaccharide proportions	1282:1317	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	0	2	theme	mulberry	86:93	arg1	Murus					103:107	Murus	103:107	Murus	103:107	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	0	2	theme	mulberry	86:93	arg1	fruits					95:100	mulberry fruits	86:100	mulberry fruits (Murus alba L.)	86:116	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	0	3	theme	grinding	174:181	arg1	pretreatment					183:194	superfine grinding pretreatment	164:194	superfine grinding pretreatment	164:194	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	2	4	from	fruits	365:370	arg1	polysaccharides					335:349	polysaccharides	335:349	polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP)	335:448	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	5	5	theme	mulberry	908:915	arg1	powder					917:922	mulberry powder	908:922	mulberry powder	908:922	The smaller particle size of mulberry powder was found to improve the extraction yields.					
34022314	0	6	with	techniques	148:157	arg1	pretreatment					183:194	superfine grinding pretreatment	164:194	superfine grinding pretreatment	164:194	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	7	7	theme	antioxidant	1507:1517	arg1	activities					1519:1528	better thixotropy and antioxidant activities	1485:1528	better thixotropy and antioxidant activities	1485:1528	MFP-HSEHE100 achieved the lowest molecular weight and particle size, which exhibited better thixotropy and antioxidant activities than other MFPs.					
34022314	1	8	theme	different	290:298	arg1	methods					311:317	different extraction methods	290:317	different extraction methods	290:317	The structural characteristics and biological activity of polysaccharides were influenced by different extraction methods.					
34022314	1	9	theme	extraction	300:309	arg1	methods					311:317	different extraction methods	290:317	different extraction methods	290:317	The structural characteristics and biological activity of polysaccharides were influenced by different extraction methods.					
34022314	2	10	theme	water	517:521	arg1	EAHE					535:538	EAHE	535:538	EAHE	535:538	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	10	theme	water	517:521	arg1	extraction					523:532	enzyme-assisted hot water extraction	497:532	enzyme-assisted hot water extraction (EAHE)	497:539	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	0	11	from	activities	51:60	arg1	Murus					103:107	Murus	103:107	Murus	103:107	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	0	11	from	activities	51:60	arg1	fruits					95:100	mulberry fruits	86:100	mulberry fruits (Murus alba L.)	86:116	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	2	12	theme	hot	636:638	arg1	HSEHE					658:662	HSEHE	658:662	HSEHE	658:662	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	12	theme	hot	636:638	arg1	extraction					646:655	high-speed shear homogenization-assisted hot water extraction	595:655	high-speed shear homogenization-assisted hot water extraction (HSEHE)	595:663	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	13	theme	water	640:644	arg1	HSEHE					658:662	HSEHE	658:662	HSEHE	658:662	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	13	theme	water	640:644	arg1	extraction					646:655	high-speed shear homogenization-assisted hot water extraction	595:655	high-speed shear homogenization-assisted hot water extraction (HSEHE)	595:663	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	0	14	dep	Murus	103:107	arg1	L.					114:115	Murus alba L.	103:115	Murus alba L.	103:115	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	2	15	theme	superfine	416:424	arg1	process					435:441	superfine grinding process	416:441	superfine grinding process	416:441	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	16	theme	hot	513:515	arg1	EAHE					535:538	EAHE	535:538	EAHE	535:538	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	16	theme	hot	513:515	arg1	extraction					523:532	enzyme-assisted hot water extraction	497:532	enzyme-assisted hot water extraction (EAHE)	497:539	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	3	17	theme	rheological	699:709	arg1	properties					711:720	rheological properties	699:720	rheological properties	699:720	The extraction yield, structure, rheological properties and antioxidant activities of MFPs were investigated.					
34022314	2	18	theme	homogenization-assisted	612:634	arg1	HSEHE					658:662	HSEHE	658:662	HSEHE	658:662	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	18	theme	homogenization-assisted	612:634	arg1	extraction					646:655	high-speed shear homogenization-assisted hot water extraction	595:655	high-speed shear homogenization-assisted hot water extraction (HSEHE)	595:663	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	7	19	theme	particle	1454:1461	arg1	size					1463:1466	particle size	1454:1466	particle size	1454:1466	MFP-HSEHE100 achieved the lowest molecular weight and particle size, which exhibited better thixotropy and antioxidant activities than other MFPs.					
34022314	7	20	theme	other	1535:1539	arg1	MFPs					1541:1544	other MFPs	1535:1544	other MFPs	1535:1544	MFP-HSEHE100 achieved the lowest molecular weight and particle size, which exhibited better thixotropy and antioxidant activities than other MFPs.					
34022314	6	21	theme	particle	1366:1373	arg1	size					1375:1378	particle size	1366:1378	particle size	1366:1378	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	2	22	theme	shear	606:610	arg1	HSEHE					658:662	HSEHE	658:662	HSEHE	658:662	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	22	theme	shear	606:610	arg1	extraction					646:655	high-speed shear homogenization-assisted hot water extraction	595:655	high-speed shear homogenization-assisted hot water extraction (HSEHE)	595:663	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	7	23	theme	molecular	1433:1441	arg1	weight					1443:1448	molecular weight	1433:1448	molecular weight	1433:1448	MFP-HSEHE100 achieved the lowest molecular weight and particle size, which exhibited better thixotropy and antioxidant activities than other MFPs.					
34022314	3	24	theme	antioxidant	726:736	arg1	activities					738:747	antioxidant activities	726:747	antioxidant activities	726:747	The extraction yield, structure, rheological properties and antioxidant activities of MFPs were investigated.					
34022314	0	25	theme	Structural	0:9	arg1	properties					24:33	rheological properties	12:33	rheological properties	12:33	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	3	26	theme	MFPs	752:755	arg1	structure					688:696	structure	688:696	structure	688:696	The extraction yield, structure, rheological properties and antioxidant activities of MFPs were investigated.					
34022314	3	26	theme	MFPs	752:755	arg1	properties					711:720	rheological properties	699:720	rheological properties	699:720	The extraction yield, structure, rheological properties and antioxidant activities of MFPs were investigated.					
34022314	3	26	theme	MFPs	752:755	arg1	yield					681:685	The extraction yield	666:685	The extraction yield	666:685	The extraction yield, structure, rheological properties and antioxidant activities of MFPs were investigated.					
34022314	3	26	theme	MFPs	752:755	arg1	activities					738:747	antioxidant activities	726:747	antioxidant activities	726:747	The extraction yield, structure, rheological properties and antioxidant activities of MFPs were investigated.					
34022314	2	27	theme	high-speed	595:604	arg1	HSEHE					658:662	HSEHE	658:662	HSEHE	658:662	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	27	theme	high-speed	595:604	arg1	extraction					646:655	high-speed shear homogenization-assisted hot water extraction	595:655	high-speed shear homogenization-assisted hot water extraction (HSEHE)	595:663	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	5	28	theme	smaller	883:889	arg1	size					900:903	The smaller particle size	879:903	The smaller particle size of mulberry powder	879:922	The smaller particle size of mulberry powder was found to improve the extraction yields.					
34022314	2	29	theme	ultrasonic-assisted	542:560	arg1	UAHE					584:587	UAHE	584:587	UAHE	584:587	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	29	theme	ultrasonic-assisted	542:560	arg1	extraction					572:581	ultrasonic-assisted hot water extraction	542:581	ultrasonic-assisted hot water extraction (UAHE)	542:588	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	6	30	theme	linkages	1228:1235	arg1	levels					1177:1182	the same levels	1168:1182	the same levels of monosaccharide compositions and glycosyl linkages	1168:1235	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	2	31	dep	Murus	373:377	arg1	L.					384:385	Murus alba L., which were pre-treated with superfine grinding process	373:441	L.	384:385	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	31	dep	Murus	373:377	arg1	pre-treated					399:409	pre-treated	399:409	were pre-treated with superfine grinding process	394:441	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	1	32	theme	structural	201:210	arg1	characteristics					212:226	The structural characteristics	197:226	The structural characteristics	197:226	The structural characteristics and biological activity of polysaccharides were influenced by different extraction methods.					
34022314	0	33	theme	rheological	12:22	arg1	properties					24:33	rheological properties	12:33	rheological properties	12:33	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	8	34	theme	suitable	1593:1600	arg1	method					1613:1618	the most suitable extraction method	1584:1618	the most suitable extraction method for MFP	1584:1626	This study identified that HSEHE was the most suitable extraction method for MFP.					
34022314	8	34	theme	suitable	1593:1600	arg1	HSEHE					1574:1578	HSEHE	1574:1578	HSEHE	1574:1578	This study identified that HSEHE was the most suitable extraction method for MFP.					
34022314	6	35	theme	molecular	1348:1356	arg1	weight					1358:1363	different molecular weight	1338:1363	different molecular weight	1338:1363	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	0	36	from	fruits	95:100	arg1	polysaccharides					65:79	polysaccharides	65:79	polysaccharides from mulberry fruits (Murus alba L.)	65:116	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	0	36	from	fruits	95:100	arg1	properties					24:33	rheological properties	12:33	rheological properties	12:33	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	0	36	from	fruits	95:100	arg1	activities					51:60	antioxidant activities	39:60	antioxidant activities	39:60	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	7	37	theme	lowest	1426:1431	arg1	weight					1443:1448	molecular weight	1433:1448	molecular weight	1433:1448	MFP-HSEHE100 achieved the lowest molecular weight and particle size, which exhibited better thixotropy and antioxidant activities than other MFPs.					
34022314	6	38	theme	grinding	1061:1068	arg1	pretreatment					1070:1081	superfine grinding pretreatment	1051:1081	superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100)	1051:1159	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	0	39	theme	antioxidant	39:49	arg1	activities					51:60	antioxidant activities	39:60	antioxidant activities	39:60	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	0	40	theme	different	127:135	arg1	techniques					148:157	different extraction techniques	127:157	different extraction techniques with superfine grinding pretreatment	127:194	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	6	41	theme	superfine	1051:1059	arg1	pretreatment					1070:1081	superfine grinding pretreatment	1051:1081	superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100)	1051:1159	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	6	42	theme	mesh	1096:1099	arg1	sieve					1101:1105	100 mesh sieve	1092:1105	100 mesh sieve	1092:1105	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	6	43	theme	different	1282:1290	arg1	proportions					1307:1317	different monosaccharide proportions	1282:1317	different monosaccharide proportions	1282:1317	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	6	44	theme	different	1338:1346	arg1	weight					1358:1363	different molecular weight	1338:1363	different molecular weight	1338:1363	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	6	45	theme	different	1018:1026	arg1	methods					1039:1045	different extraction methods	1018:1045	different extraction methods	1018:1045	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	4	46	contain	have	813:816	arg2	yields					841:846	the highest extraction yields	818:846	the highest extraction yields	818:846	MFP extracted using the HSEHE method have the highest extraction yields than other extraction methods.					
34022314	4	46	contain	have	813:816	arg1	MFP					776:778	MFP	776:778	MFP extracted using the HSEHE method	776:811	MFP extracted using the HSEHE method have the highest extraction yields than other extraction methods.					
34022314	1	47	theme	biological	232:241	arg1	activity					243:250	biological activity	232:250	biological activity	232:250	The structural characteristics and biological activity of polysaccharides were influenced by different extraction methods.					
34022314	2	48	theme	hot-water	469:477	arg1	HWE					491:493	HWE	491:493	HWE	491:493	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	48	theme	hot-water	469:477	arg1	extraction					479:488	hot-water extraction	469:488	hot-water extraction (HWE)	469:494	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	8	49	theme	extraction	1602:1611	arg1	method					1613:1618	the most suitable extraction method	1584:1618	the most suitable extraction method for MFP	1584:1626	This study identified that HSEHE was the most suitable extraction method for MFP.					
34022314	8	49	theme	extraction	1602:1611	arg1	HSEHE					1574:1578	HSEHE	1574:1578	HSEHE	1574:1578	This study identified that HSEHE was the most suitable extraction method for MFP.					
34022314	4	50	theme	extraction	830:839	arg1	yields					841:846	the highest extraction yields	818:846	the highest extraction yields	818:846	MFP extracted using the HSEHE method have the highest extraction yields than other extraction methods.					
34022314	0	51	theme	superfine	164:172	arg1	pretreatment					183:194	superfine grinding pretreatment	164:194	superfine grinding pretreatment	164:194	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	5	52	theme	powder	917:922	arg1	size					900:903	The smaller particle size	879:903	The smaller particle size of mulberry powder	879:922	The smaller particle size of mulberry powder was found to improve the extraction yields.					
34022314	6	53	dep	pretreatment	1070:1081	arg1	MFP-HSEHE100					1147:1158	MFP-HSEHE100	1147:1158	MFP-HSEHE100	1147:1158	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	0	54	theme	extraction	137:146	arg1	techniques					148:157	different extraction techniques	127:157	different extraction techniques with superfine grinding pretreatment	127:194	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	4	55	theme	highest	822:828	arg1	yields					841:846	the highest extraction yields	818:846	the highest extraction yields	818:846	MFP extracted using the HSEHE method have the highest extraction yields than other extraction methods.					
34022314	6	56	theme	monosaccharide	1187:1200	arg1	compositions					1202:1213	monosaccharide compositions	1187:1213	monosaccharide compositions	1187:1213	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	2	57	theme	water	566:570	arg1	UAHE					584:587	UAHE	584:587	UAHE	584:587	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	57	theme	water	566:570	arg1	extraction					572:581	ultrasonic-assisted hot water extraction	542:581	ultrasonic-assisted hot water extraction (UAHE)	542:588	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	3	58	theme	extraction	670:679	arg1	yield					681:685	The extraction yield	666:685	The extraction yield	666:685	The extraction yield, structure, rheological properties and antioxidant activities of MFPs were investigated.					
34022314	6	59	theme	glycosyl	1219:1226	arg1	linkages					1228:1235	glycosyl linkages	1219:1235	glycosyl linkages	1219:1235	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	7	60	theme	thixotropy	1492:1501	arg1	activities					1519:1528	better thixotropy and antioxidant activities	1485:1528	better thixotropy and antioxidant activities	1485:1528	MFP-HSEHE100 achieved the lowest molecular weight and particle size, which exhibited better thixotropy and antioxidant activities than other MFPs.					
34022314	5	61	theme	extraction	949:958	arg1	yields					960:965	the extraction yields	945:965	the extraction yields	945:965	The smaller particle size of mulberry powder was found to improve the extraction yields.					
34022314	2	62	theme	hot	562:564	arg1	UAHE					584:587	UAHE	584:587	UAHE	584:587	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	62	theme	hot	562:564	arg1	extraction					572:581	ultrasonic-assisted hot water extraction	542:581	ultrasonic-assisted hot water extraction (UAHE)	542:588	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	4	63	theme	extraction	859:868	arg1	methods					870:876	other extraction methods	853:876	other extraction methods	853:876	MFP extracted using the HSEHE method have the highest extraction yields than other extraction methods.					
34022314	6	64	theme	same	1172:1175	arg1	levels					1177:1182	the same levels	1168:1182	the same levels of monosaccharide compositions and glycosyl linkages	1168:1235	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	2	65	theme	mulberry	356:363	arg1	MFP					445:447	MFP	445:447	MFP	445:447	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	65	theme	mulberry	356:363	arg1	Murus					373:377	Murus	373:377	Murus	373:377	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	65	theme	mulberry	356:363	arg1	fruits					365:370	mulberry fruits	356:370	mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP)	356:448	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	6	66	theme	extraction	1028:1037	arg1	methods					1039:1045	different extraction methods	1018:1045	different extraction methods	1018:1045	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	2	67	theme	enzyme-assisted	497:511	arg1	EAHE					535:538	EAHE	535:538	EAHE	535:538	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	67	theme	enzyme-assisted	497:511	arg1	extraction					523:532	enzyme-assisted hot water extraction	497:532	enzyme-assisted hot water extraction (EAHE)	497:539	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	2	68	theme	grinding	426:433	arg1	process					435:441	superfine grinding process	416:441	superfine grinding process	416:441	In this study, polysaccharides from mulberry fruits (Murus alba L., which were pre-treated with superfine grinding process) (MFP) were exacted using hot-water extraction (HWE), enzyme-assisted hot water extraction (EAHE), ultrasonic-assisted hot water extraction (UAHE), and high-speed shear homogenization-assisted hot water extraction (HSEHE).					
34022314	6	69	theme	compositions	1202:1213	arg1	levels					1177:1182	the same levels	1168:1182	the same levels of monosaccharide compositions and glycosyl linkages	1168:1235	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	5	70	theme	particle	891:898	arg1	size					900:903	The smaller particle size	879:903	The smaller particle size of mulberry powder	879:922	The smaller particle size of mulberry powder was found to improve the extraction yields.					
34022314	6	71	theme	100	1092:1094	arg1	mesh					1096:1099	mesh	1096:1099	mesh	1096:1099	The MFPs were obtained by the combination between different extraction methods and superfine grinding pretreatment (through 100 mesh sieve) (MFP-HWE100, MFP-EAHE100, MFP-UAHE100, MFP-HSEHE100) showed the same levels of monosaccharide compositions and glycosyl linkages, However, these methods can produce MFP with different monosaccharide proportions, branching degree, different molecular weight, particle size and microstructure.					
34022314	1	72	theme	polysaccharides	255:269	arg1	characteristics					212:226	The structural characteristics	197:226	The structural characteristics	197:226	The structural characteristics and biological activity of polysaccharides were influenced by different extraction methods.					
34022314	1	72	theme	polysaccharides	255:269	arg1	activity					243:250	biological activity	232:250	biological activity	232:250	The structural characteristics and biological activity of polysaccharides were influenced by different extraction methods.					
34022314	0	73	theme	polysaccharides	65:79	arg1	properties					24:33	rheological properties	12:33	rheological properties	12:33	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	0	73	theme	polysaccharides	65:79	arg1	activities					51:60	antioxidant activities	39:60	antioxidant activities	39:60	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	0	74	from	properties	24:33	arg1	Murus					103:107	Murus	103:107	Murus	103:107	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	0	74	from	properties	24:33	arg1	fruits					95:100	mulberry fruits	86:100	mulberry fruits (Murus alba L.)	86:116	Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment.					
34022314	4	75	theme	HSEHE	800:804	arg1	method					806:811	the HSEHE method	796:811	the HSEHE method	796:811	MFP extracted using the HSEHE method have the highest extraction yields than other extraction methods.					
34022314	4	76	theme	other	853:857	arg1	methods					870:876	other extraction methods	853:876	other extraction methods	853:876	MFP extracted using the HSEHE method have the highest extraction yields than other extraction methods.					
34775751	7	0	theme	simulation	1440:1449	arg1	methods					1451:1457	simulation methods	1440:1457	simulation methods	1440:1457	The conformational behavior was also investigated by NMR and molecular dynamics simulation methods and compared with data reported for natural fCS.					
34775751	1	1	theme	chondroitin	178:188	arg1	fCS					199:201	fCS	199:201	fCS	199:201	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	1	1	theme	chondroitin	178:188	arg1	sulfate					190:196	fucosylated chondroitin sulfate	166:196	fucosylated chondroitin sulfate (fCS)	166:202	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	3	2	dep	fucosylation	747:758	arg1	the					728:730	the	728:730	the	728:730	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	1	3	with	polysaccharides	327:341	arg1	structure					363:371	a well-defined structure	348:371	a well-defined structure	348:371	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	5	4	theme	glycosylation	1165:1177	arg1	reaction					1179:1186	a glycosylation reaction	1163:1186	a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step	1163:1267	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	2	5	theme	controlled	514:523	arg1	synthesis					500:508	Total synthesis	494:508	Total synthesis	494:508	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	2	5	theme	controlled	514:523	arg1	depolymerization					525:540	controlled depolymerization	514:540	controlled depolymerization	514:540	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	2	5	theme	controlled	514:523	arg1	approaches					590:599	the main approaches	581:599	the main approaches to this aim	581:611	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	1	6	theme	well-defined	350:361	arg1	structure					363:371	a well-defined structure	348:371	a well-defined structure	348:371	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	5	7	theme	fCS	1116:1118	arg1	isomers					1120:1126	some structurally homogeneous fCS isomers	1086:1126	the first time some structurally homogeneous fCS isomers	1071:1126	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	3	8	theme	fucose	875:880	arg1	devoid					861:866	devoid	861:866	devoid	861:866	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	3	9	theme	same	824:827	arg1	backbone					841:848	the same chondroitin backbone	820:848	the same chondroitin backbone of fCS	820:855	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	0	10	theme	Non-Natural	100:110	arg1	Site					112:115	a Non-Natural Site	98:115	a Non-Natural Site	98:115	Semisynthetic Isomers of Fucosylated Chondroitin Sulfate Polysaccharides with Fucosyl Branches at a Non-Natural Site.					
34775751	2	11	theme	main	585:588	arg1	synthesis					500:508	Total synthesis	494:508	Total synthesis	494:508	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	2	11	theme	main	585:588	arg1	depolymerization					525:540	controlled depolymerization	514:540	controlled depolymerization	514:540	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	2	11	theme	main	585:588	arg1	approaches					590:599	the main approaches	581:599	the main approaches to this aim	581:611	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	1	12	theme	native	449:454	arg1	fCSs					478:481	native, sea cucumber sourced fCSs	449:481	native, sea cucumber sourced fCSs as a drug	449:491	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	3	13	from	fucose	875:880	arg1	it					914:915	it	914:915	it	914:915	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	3	14	theme	sulfate	892:898	arg1	decoration					900:909	sulfate decoration	892:909	sulfate decoration	892:909	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	1	15	theme	fCS	275:277	arg1	oligosaccharides					279:294	fCS oligosaccharides	275:294	fCS oligosaccharides	275:294	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	6	16	theme	structural	1321:1330	arg1	characterization					1332:1347	a detailed structural characterization	1310:1347	a detailed structural characterization by 2D-NMR	1310:1357	The obtained products were subjected to a detailed structural characterization by 2D-NMR.					
34775751	3	17	gly	fucosylation	747:758	arg1	polysaccharide					797:810	a microbial sourced polysaccharide	777:810	a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it	777:915	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	3	18	theme	decoration	900:909	arg1	devoid					861:866	devoid	861:866	devoid	861:866	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	2	19	theme	Total	494:498	arg1	synthesis					500:508	Total synthesis	494:508	Total synthesis	494:508	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	2	19	theme	Total	494:498	arg1	depolymerization					525:540	controlled depolymerization	514:540	controlled depolymerization	514:540	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	2	19	theme	Total	494:498	arg1	approaches					590:599	the main approaches	581:599	the main approaches to this aim	581:611	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	6	20	theme	detailed	1312:1319	arg1	characterization					1332:1347	a detailed structural characterization	1310:1347	a detailed structural characterization by 2D-NMR	1310:1357	The obtained products were subjected to a detailed structural characterization by 2D-NMR.					
34775751	1	21	theme	sea	457:459	arg1	cucumber					461:468	sea cucumber	457:468	sea cucumber	457:468	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	4	22	theme	fCS	985:987	arg1	isomers					989:995	fCS isomers	985:995	fCS isomers carrying Fuc and sulfate groups at non-natural sites	985:1048	This approach is highly versatile, as it could open access also to fCS isomers carrying Fuc and sulfate groups at non-natural sites.					
34775751	5	23	theme	different	1236:1244	arg1	donors					1250:1255	three different Fuc donors	1230:1255	three different Fuc donors	1230:1255	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	3	24	theme	regioselective	732:745	arg1	fucosylation					747:758	regioselective fucosylation	732:758	regioselective fucosylation	732:758	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	5	25	theme	polysaccharide	1202:1215	arg1	acceptor					1217:1224	a LMW polysaccharide acceptor	1196:1224	a LMW polysaccharide acceptor	1196:1224	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	6	26	theme	obtained	1274:1281	arg1	products					1283:1290	The obtained products	1270:1290	The obtained products	1270:1290	The obtained products were subjected to a detailed structural characterization by 2D-NMR.					
34775751	0	27	theme	Semisynthetic	0:12	arg1	Isomers					14:20	Semisynthetic Isomers	0:20	Semisynthetic Isomers of Fucosylated Chondroitin Sulfate	0:55	Semisynthetic Isomers of Fucosylated Chondroitin Sulfate Polysaccharides with Fucosyl Branches at a Non-Natural Site.					
34775751	1	28	theme	oligosaccharides	279:294	arg1	obtainment					261:270	the obtainment	257:270	the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure	257:371	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	7	29	theme	natural	1495:1501	arg1	fCS					1503:1505	natural fCS	1495:1505	natural fCS	1495:1505	The conformational behavior was also investigated by NMR and molecular dynamics simulation methods and compared with data reported for natural fCS.					
34775751	1	30	with	oligosaccharides	279:294	arg1	structure					363:371	a well-defined structure	348:371	a well-defined structure	348:371	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	0	31	theme	Fucosylated	25:35	arg1	Sulfate					49:55	Fucosylated Chondroitin Sulfate	25:55	Fucosylated Chondroitin Sulfate	25:55	Semisynthetic Isomers of Fucosylated Chondroitin Sulfate Polysaccharides with Fucosyl Branches at a Non-Natural Site.					
34775751	5	32	theme	multistep	1138:1146	arg1	procedure					1148:1156	a multistep procedure	1136:1156	a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step	1136:1267	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	2	33	theme	natural	549:555	arg1	polysaccharides					561:575	the natural fCS polysaccharides	545:575	the natural fCS polysaccharides	545:575	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	1	34	theme	fCSs	478:481	arg1	employment					435:444	the potential employment	421:444	the potential employment of native, sea cucumber sourced fCSs as a drug	421:491	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	3	35	theme	chondroitin	829:839	arg1	backbone					841:848	the same chondroitin backbone	820:848	the same chondroitin backbone of fCS	820:855	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	1	36	theme	low	300:302	arg1	LMW					322:324	LMW	322:324	LMW	322:324	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	1	36	theme	low	300:302	arg1	weight					314:319	low molecular weight	300:319	low molecular weight (LMW) polysaccharides	300:341	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	5	37	theme	key	1260:1262	arg1	step					1264:1267	key step	1260:1267	key step	1260:1267	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	5	38	theme	Fuc	1246:1248	arg1	donors					1250:1255	three different Fuc donors	1230:1255	three different Fuc donors	1230:1255	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	0	39	theme	Sulfate	49:55	arg1	Isomers					14:20	Semisynthetic Isomers	0:20	Semisynthetic Isomers of Fucosylated Chondroitin Sulfate	0:55	Semisynthetic Isomers of Fucosylated Chondroitin Sulfate Polysaccharides with Fucosyl Branches at a Non-Natural Site.					
34775751	1	40	theme	molecular	304:312	arg1	LMW					322:324	LMW	322:324	LMW	322:324	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	1	40	theme	molecular	304:312	arg1	weight					314:319	low molecular weight	300:319	low molecular weight (LMW) polysaccharides	300:341	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	3	41	dep	polysaccharide	797:810	arg1	sharing					812:818	sharing	812:818	sharing the same chondroitin backbone of fCS	812:855	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	3	41	dep	polysaccharide	797:810	arg1	devoid					861:866	devoid	861:866	devoid	861:866	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	5	42	with	procedure	1148:1156	arg1	reaction					1179:1186	a glycosylation reaction	1163:1186	a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step	1163:1267	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	7	43	theme	molecular	1421:1429	arg1	dynamics					1431:1438	molecular dynamics	1421:1438	molecular dynamics	1421:1438	The conformational behavior was also investigated by NMR and molecular dynamics simulation methods and compared with data reported for natural fCS.					
34775751	0	44	theme	Chondroitin	37:47	arg1	Sulfate					49:55	Fucosylated Chondroitin Sulfate	25:55	Fucosylated Chondroitin Sulfate	25:55	Semisynthetic Isomers of Fucosylated Chondroitin Sulfate Polysaccharides with Fucosyl Branches at a Non-Natural Site.					
34775751	1	45	theme	weight	314:319	arg1	polysaccharides					327:341	low molecular weight (LMW) polysaccharides	300:341	low molecular weight (LMW) polysaccharides	300:341	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	4	46	theme	Fuc	1006:1008	arg1	groups					1022:1027	Fuc and sulfate groups	1006:1027	Fuc and sulfate groups	1006:1027	This approach is highly versatile, as it could open access also to fCS isomers carrying Fuc and sulfate groups at non-natural sites.					
34775751	1	47	theme	last	223:226	arg1	years					228:232	the last years	219:232	the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure	219:371	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	3	48	theme	microbial	779:787	arg1	polysaccharide					797:810	a microbial sourced polysaccharide	777:810	a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it	777:915	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	1	49	gly	fucosylated	166:176	arg1	fCS					199:201	fCS	199:201	fCS	199:201	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	1	49	gly	fucosylated	166:176	arg1	sulfate					190:196	fucosylated chondroitin sulfate	166:196	fucosylated chondroitin sulfate (fCS)	166:202	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	7	50	dep	NMR	1413:1415	arg1	methods					1451:1457	simulation methods	1440:1457	simulation methods	1440:1457	The conformational behavior was also investigated by NMR and molecular dynamics simulation methods and compared with data reported for natural fCS.					
34775751	1	51	theme	several	122:128	arg1	activities					142:151	The several interesting activities	118:151	The several interesting activities detected for fucosylated chondroitin sulfate (fCS)	118:202	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	5	52	theme	first	1075:1079	arg1	time					1081:1084	the first time some structurally homogeneous fCS isomers	1071:1126	the first time some structurally homogeneous fCS isomers	1071:1126	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	5	53	dep	time	1081:1084	arg1	isomers					1120:1126	some structurally homogeneous fCS isomers	1086:1126	the first time some structurally homogeneous fCS isomers	1071:1126	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	5	54	theme	homogeneous	1104:1114	arg1	isomers					1120:1126	some structurally homogeneous fCS isomers	1086:1126	the first time some structurally homogeneous fCS isomers	1071:1126	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	1	55	theme	interesting	130:140	arg1	activities					142:151	The several interesting activities	118:151	The several interesting activities detected for fucosylated chondroitin sulfate (fCS)	118:202	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	1	56	dep	native	449:454	arg1	sourced					470:476	sourced	470:476	sourced	470:476	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	5	57	theme	LMW	1198:1200	arg1	acceptor					1217:1224	a LMW polysaccharide acceptor	1196:1224	a LMW polysaccharide acceptor	1196:1224	Here we prepare for the first time some structurally homogeneous fCS isomers through a multistep procedure with a glycosylation reaction between a LMW polysaccharide acceptor and three different Fuc donors as key step.					
34775751	2	58	theme	polysaccharides	561:575	arg1	synthesis					500:508	Total synthesis	494:508	Total synthesis	494:508	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	2	58	theme	polysaccharides	561:575	arg1	depolymerization					525:540	controlled depolymerization	514:540	controlled depolymerization	514:540	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	2	58	theme	polysaccharides	561:575	arg1	approaches					590:599	the main approaches	581:599	the main approaches to this aim	581:611	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	4	59	contain	carrying	997:1004	arg1	isomers					989:995	fCS isomers	985:995	fCS isomers carrying Fuc and sulfate groups at non-natural sites	985:1048	This approach is highly versatile, as it could open access also to fCS isomers carrying Fuc and sulfate groups at non-natural sites.					
34775751	4	59	contain	carrying	997:1004	arg2	groups					1022:1027	Fuc and sulfate groups	1006:1027	Fuc and sulfate groups	1006:1027	This approach is highly versatile, as it could open access also to fCS isomers carrying Fuc and sulfate groups at non-natural sites.					
34775751	7	60	theme	conformational	1364:1377	arg1	behavior					1379:1386	The conformational behavior	1360:1386	The conformational behavior	1360:1386	The conformational behavior was also investigated by NMR and molecular dynamics simulation methods and compared with data reported for natural fCS.					
34775751	4	61	theme	non-natural	1032:1042	arg1	sites					1044:1048	non-natural sites	1032:1048	non-natural sites	1032:1048	This approach is highly versatile, as it could open access also to fCS isomers carrying Fuc and sulfate groups at non-natural sites.					
34775751	1	62	theme	years	228:232	arg1	efforts					242:248	the last years several efforts	219:248	the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure	219:371	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	2	63	theme	fCS	557:559	arg1	polysaccharides					561:575	the natural fCS polysaccharides	545:575	the natural fCS polysaccharides	545:575	Total synthesis and controlled depolymerization of the natural fCS polysaccharides are the main approaches to this aim; nonetheless, they present some limitations.					
34775751	0	64	from	Site	112:115	arg1	Branches					86:93	Fucosyl Branches	78:93	Fucosyl Branches at a Non-Natural Site	78:115	Semisynthetic Isomers of Fucosylated Chondroitin Sulfate Polysaccharides with Fucosyl Branches at a Non-Natural Site.					
34775751	3	65	theme	fCS	853:855	arg1	backbone					841:848	the same chondroitin backbone	820:848	the same chondroitin backbone of fCS	820:855	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	1	66	theme	several	234:240	arg1	efforts					242:248	the last years several efforts	219:248	the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure	219:371	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	4	67	theme	sulfate	1014:1020	arg1	groups					1022:1027	Fuc and sulfate groups	1006:1027	Fuc and sulfate groups	1006:1027	This approach is highly versatile, as it could open access also to fCS isomers carrying Fuc and sulfate groups at non-natural sites.					
34775751	1	68	theme	potential	425:433	arg1	employment					435:444	the potential employment	421:444	the potential employment of native, sea cucumber sourced fCSs as a drug	421:491	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	0	69	with	Polysaccharides	57:71	arg1	Branches					86:93	Fucosyl Branches	78:93	Fucosyl Branches at a Non-Natural Site	78:115	Semisynthetic Isomers of Fucosylated Chondroitin Sulfate Polysaccharides with Fucosyl Branches at a Non-Natural Site.					
34775751	3	70	theme	sourced	789:795	arg1	polysaccharide					797:810	a microbial sourced polysaccharide	777:810	a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it	777:915	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	0	71	theme	Fucosyl	78:84	arg1	Branches					86:93	Fucosyl Branches	78:93	Fucosyl Branches at a Non-Natural Site	78:115	Semisynthetic Isomers of Fucosylated Chondroitin Sulfate Polysaccharides with Fucosyl Branches at a Non-Natural Site.					
34775751	1	72	theme	polysaccharides	327:341	arg1	obtainment					261:270	the obtainment	257:270	the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure	257:371	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	3	73	from	decoration	900:909	arg1	it					914:915	it	914:915	it	914:915	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	1	74	theme	fucosylated	166:176	arg1	fCS					199:201	fCS	199:201	fCS	199:201	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	1	74	theme	fucosylated	166:176	arg1	sulfate					190:196	fucosylated chondroitin sulfate	166:196	fucosylated chondroitin sulfate (fCS)	166:202	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	1	75	dep	sourced	470:476	arg1	cucumber					461:468	sea cucumber	457:468	sea cucumber	457:468	The several interesting activities detected for fucosylated chondroitin sulfate (fCS) have fueled in the last years several efforts toward the obtainment of fCS oligosaccharides and low molecular weight (LMW) polysaccharides with a well-defined structure, in order to avoid the problems associated with the potential employment of native, sea cucumber sourced fCSs as a drug.					
34775751	3	76	theme	polysaccharide	797:810	arg1	fucosylation					747:758	regioselective fucosylation	732:758	regioselective fucosylation	732:758	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
34775751	3	76	theme	polysaccharide	797:810	arg1	sulfation					764:772	sulfation	764:772	sulfation	764:772	These could be circumvented by semisynthesis, a strategy relying upon the regioselective fucosylation and sulfation of a microbial sourced polysaccharide sharing the same chondroitin backbone of fCS but devoid of any fucose (Fuc) and sulfate decoration on it.					
33065988	8	0	gly	glycoproteins	1437:1449	arg1	glycoproteins					1437:1449	glycoproteins	1437:1449	glycoproteins	1437:1449	Therefore, HILIC is a useful tool for the comprehensive characterization of glycoproteins and their isomers.					
33065988	3	1	theme	isomers	542:548	arg1	separation					504:513	the separation	500:513	the separation of glycopeptides and their isomers	500:548	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	4	2	theme	Glycan	617:622	arg1	Amide					654:658	Glycan ethylene bridged hybrid (BEH) Amide	617:658	Glycan ethylene bridged hybrid (BEH) Amide	617:658	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	0	3	theme	Hemopexin	69:77	arg1	Glycopeptides					100:112	Hemopexin and Immunoglobulin G Glycopeptides	69:112	Hemopexin and Immunoglobulin G Glycopeptides	69:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	4	4	theme	different	557:565	arg1	phases					584:589	Three different HILIC stationary phases	551:589	Three different HILIC stationary phases	551:589	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	5	5	theme	ZIC-HILIC	942:950	arg1	column					952:957	the ZIC-HILIC column	938:957	the ZIC-HILIC column	938:957	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	0	6	theme	G	98:98	arg1	Glycopeptides					100:112	Hemopexin and Immunoglobulin G Glycopeptides	69:112	Hemopexin and Immunoglobulin G Glycopeptides	69:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	0	7	from	Comparison	0:9	arg1	Separation					55:64	the Separation	51:64	the Separation of Hemopexin and Immunoglobulin G Glycopeptides	51:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	5	8	theme	sialic	971:976	arg1	acid					978:981	sialic acid	971:981	sialic acid	971:981	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	4	9	theme	glycoproteins	769:781	arg1	N-glycopeptides					719:733	complex N-glycopeptides	711:733	complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins	711:781	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	4	10	theme	bridged	633:639	arg1	Amide					654:658	Glycan ethylene bridged hybrid (BEH) Amide	617:658	Glycan ethylene bridged hybrid (BEH) Amide	617:658	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	0	11	theme	Immunoglobulin	83:96	arg1	G					98:98	Immunoglobulin G	83:98	Immunoglobulin G	83:98	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	4	12	dep	penta-HILIC	604:614	arg1	i.e.					592:595	i.e.	592:595	i.e.	592:595	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	4	13	theme	complex	711:717	arg1	N-glycopeptides					719:733	complex N-glycopeptides	711:733	complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins	711:781	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	3	14	theme	glycopeptides	518:530	arg1	separation					504:513	the separation	500:513	the separation of glycopeptides and their isomers	500:548	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	4	15	gly	glycoproteins	769:781	arg1	glycoproteins					769:781	hemopexin and Immunoglobulin G glycoproteins	738:781	hemopexin and Immunoglobulin G glycoproteins	738:781	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	4	16	theme	ethylene	624:631	arg1	Amide					654:658	Glycan ethylene bridged hybrid (BEH) Amide	617:658	Glycan ethylene bridged hybrid (BEH) Amide	617:658	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	7	17	theme	glycoforms	1349:1358	arg1	separation					1308:1317	the isomeric separation	1295:1317	the isomeric separation of fucosylated and sialylated glycoforms	1295:1358	Moreover, we showed the potential of these HILIC columns for the isomeric separation of fucosylated and sialylated glycoforms.					
33065988	0	18	theme	Glycopeptides	100:112	arg1	Separation					55:64	the Separation	51:64	the Separation of Hemopexin and Immunoglobulin G Glycopeptides	51:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	0	19	from	Phases	41:46	arg1	Separation					55:64	the Separation	51:64	the Separation of Hemopexin and Immunoglobulin G Glycopeptides	51:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	3	20	theme	liquid	450:455	arg1	HILIC					473:477	HILIC	473:477	HILIC	473:477	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	3	20	theme	liquid	450:455	arg1	chromatography					457:470	hydrophilic interaction liquid chromatography	426:470	a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase	415:495	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	7	21	theme	fucosylated	1322:1332	arg1	separation					1308:1317	the isomeric separation	1295:1317	the isomeric separation of fucosylated and sialylated glycoforms	1295:1358	Moreover, we showed the potential of these HILIC columns for the isomeric separation of fucosylated and sialylated glycoforms.					
33065988	2	22	theme	tryptic	292:298	arg1	peptides					300:307	tryptic peptides	292:307	tryptic peptides	292:307	However, chromatographically separating glycans attached to tryptic peptides enables their site-specific characterization.					
33065988	3	23	theme	stationary	480:489	arg1	phase					491:495	a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase	415:495	a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase	415:495	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	8	24	theme	glycoproteins	1437:1449	arg1	characterization					1417:1432	the comprehensive characterization	1399:1432	the comprehensive characterization of glycoproteins and their isomers	1399:1467	Therefore, HILIC is a useful tool for the comprehensive characterization of glycoproteins and their isomers.					
33065988	7	25	gly	sialylated	1338:1347	arg1	glycoforms					1349:1358	sialylated glycoforms	1338:1358	sialylated glycoforms	1338:1358	Moreover, we showed the potential of these HILIC columns for the isomeric separation of fucosylated and sialylated glycoforms.					
33065988	4	26	theme	G	767:767	arg1	glycoproteins					769:781	hemopexin and Immunoglobulin G glycoproteins	738:781	hemopexin and Immunoglobulin G glycoproteins	738:781	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	7	27	theme	columns	1283:1289	arg1	potential					1258:1266	the potential	1254:1266	the potential of these HILIC columns for the isomeric separation of fucosylated and sialylated glycoforms	1254:1358	Moreover, we showed the potential of these HILIC columns for the isomeric separation of fucosylated and sialylated glycoforms.					
33065988	5	28	theme	HILIC	891:895	arg1	columns					897:903	all HILIC columns	887:903	all HILIC columns tested in this study	887:924	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	3	29	theme	suitable	417:424	arg1	phase					491:495	a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase	415:495	a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase	415:495	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	5	30	with	repulsion	1032:1040	arg1	group					1094:1098	the negatively charged sulfobetaine functional group	1047:1098	the negatively charged sulfobetaine functional group	1047:1098	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	4	31	theme	hemopexin	738:746	arg1	glycoproteins					769:781	hemopexin and Immunoglobulin G glycoproteins	738:781	hemopexin and Immunoglobulin G glycoproteins	738:781	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	4	32	theme	Immunoglobulin	752:765	arg1	G					767:767	Immunoglobulin G	752:767	Immunoglobulin G	752:767	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	7	33	theme	isomeric	1299:1306	arg1	separation					1308:1317	the isomeric separation	1295:1317	the isomeric separation of fucosylated and sialylated glycoforms	1295:1358	Moreover, we showed the potential of these HILIC columns for the isomeric separation of fucosylated and sialylated glycoforms.					
33065988	1	34	theme	structural	190:199	arg1	variety					201:207	the structural variety	186:207	the structural variety of complex conjugates	186:229	Protein glycosylation analysis is challenging due to the structural variety of complex conjugates.					
33065988	1	34	theme	structural	190:199	arg1	conjugates					220:229	complex conjugates	212:229	complex conjugates	212:229	Protein glycosylation analysis is challenging due to the structural variety of complex conjugates.					
33065988	0	35	theme	HILIC	24:28	arg1	Phases					41:46	Different HILIC Stationary Phases	14:46	Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides	14:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	5	36	theme	glycans	838:844	arg1	polarity					822:829	the polarity	818:829	the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study	818:924	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	4	37	gly	N-glycopeptides	719:733	arg1	glycoproteins					769:781	hemopexin and Immunoglobulin G glycoproteins	738:781	hemopexin and Immunoglobulin G glycoproteins	738:781	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	4	37	gly	N-glycopeptides	719:733	arg2	N-glycopeptides					719:733	complex N-glycopeptides	711:733	complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins	711:781	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	5	38	attach	attached	846:853	arg2	glycans					838:844	the glycans	834:844	the glycans attached to the same peptide backbone in all HILIC columns tested in this study	834:924	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	5	38	attach	attached	846:853	arg1	backbone					875:882	the same peptide backbone	858:882	the same peptide backbone in all HILIC columns tested in this study	858:924	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	3	39	gly	glycopeptides	518:530	arg2	glycopeptides					518:530	glycopeptides	518:530	glycopeptides	518:530	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	0	40	theme	Different	14:22	arg1	Phases					41:46	Different HILIC Stationary Phases	14:46	Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides	14:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	5	41	theme	functional	1083:1092	arg1	group					1094:1098	the negatively charged sulfobetaine functional group	1047:1098	the negatively charged sulfobetaine functional group	1047:1098	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	2	42	theme	site-specific	323:335	arg1	characterization					337:352	their site-specific characterization	317:352	their site-specific characterization	317:352	However, chromatographically separating glycans attached to tryptic peptides enables their site-specific characterization.					
33065988	5	43	theme	same	862:865	arg1	backbone					875:882	the same peptide backbone	858:882	the same peptide backbone in all HILIC columns tested in this study	858:924	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	0	44	theme	Phases	41:46	arg1	Comparison					0:9	Comparison	0:9	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides	0:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	0	44	theme	Phases	41:46	arg1	Isomers					124:130	Their Isomers	118:130	Their Isomers	118:130	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	4	45	theme	HALO®	598:602	arg1	penta-HILIC					604:614	HALO® penta-HILIC	598:614	HALO® penta-HILIC	598:614	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	5	46	theme	peptide	867:873	arg1	backbone					875:882	the same peptide backbone	858:882	the same peptide backbone in all HILIC columns tested in this study	858:924	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	8	47	theme	comprehensive	1403:1415	arg1	characterization					1417:1432	the comprehensive characterization	1399:1432	the comprehensive characterization of glycoproteins and their isomers	1399:1467	Therefore, HILIC is a useful tool for the comprehensive characterization of glycoproteins and their isomers.					
33065988	7	48	theme	sialylated	1338:1347	arg1	glycoforms					1349:1358	sialylated glycoforms	1338:1358	sialylated glycoforms	1338:1358	Moreover, we showed the potential of these HILIC columns for the isomeric separation of fucosylated and sialylated glycoforms.					
33065988	1	49	theme	complex	212:218	arg1	conjugates					220:229	complex conjugates	212:229	complex conjugates	212:229	Protein glycosylation analysis is challenging due to the structural variety of complex conjugates.					
33065988	0	50	theme	Stationary	30:39	arg1	Phases					41:46	Different HILIC Stationary Phases	14:46	Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides	14:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	4	51	theme	stationary	573:582	arg1	phases					584:589	Three different HILIC stationary phases	551:589	Three different HILIC stationary phases	551:589	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	3	52	theme	chromatography	457:470	arg1	phase					491:495	a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase	415:495	a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase	415:495	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	5	53	theme	electrostatic	1018:1030	arg1	repulsion					1032:1040	electrostatic repulsion	1018:1040	electrostatic repulsion with the negatively charged sulfobetaine functional group	1018:1098	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	1	54	theme	conjugates	220:229	arg1	variety					201:207	the structural variety	186:207	the structural variety of complex conjugates	186:229	Protein glycosylation analysis is challenging due to the structural variety of complex conjugates.					
33065988	1	54	theme	conjugates	220:229	arg1	conjugates					220:229	complex conjugates	212:229	complex conjugates	212:229	Protein glycosylation analysis is challenging due to the structural variety of complex conjugates.					
33065988	3	55	theme	hydrophilic	426:436	arg1	HILIC					473:477	HILIC	473:477	HILIC	473:477	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	3	55	theme	hydrophilic	426:436	arg1	chromatography					457:470	hydrophilic interaction liquid chromatography	426:470	a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase	415:495	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	4	56	theme	HILIC	567:571	arg1	phases					584:589	Three different HILIC stationary phases	551:589	Three different HILIC stationary phases	551:589	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	8	57	theme	useful	1383:1388	arg1	HILIC					1372:1376	HILIC	1372:1376	HILIC	1372:1376	Therefore, HILIC is a useful tool for the comprehensive characterization of glycoproteins and their isomers.					
33065988	8	57	theme	useful	1383:1388	arg1	tool					1390:1393	a useful tool	1381:1393	a useful tool for the comprehensive characterization of glycoproteins and their isomers	1381:1467	Therefore, HILIC is a useful tool for the comprehensive characterization of glycoproteins and their isomers.					
33065988	0	58	from	Separation	55:64	arg1	Comparison					0:9	Comparison	0:9	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides	0:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	0	58	from	Separation	55:64	arg1	Isomers					124:130	Their Isomers	118:130	Their Isomers	118:130	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	5	59	from	backbone	875:882	arg1	columns					897:903	all HILIC columns	887:903	all HILIC columns tested in this study	887:924	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	3	60	theme	interaction	438:448	arg1	HILIC					473:477	HILIC	473:477	HILIC	473:477	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	3	60	theme	interaction	438:448	arg1	chromatography					457:470	hydrophilic interaction liquid chromatography	426:470	a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase	415:495	For this purpose, we have shown the importance of selecting a suitable hydrophilic interaction liquid chromatography (HILIC) stationary phase in the separation of glycopeptides and their isomers.					
33065988	4	61	theme	N-glycopeptides	719:733	arg1	separation					697:706	the separation	693:706	the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins	693:781	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	5	62	theme	glycan	990:995	arg1	moiety					997:1002	the glycan moiety	986:1002	the glycan moiety	986:1002	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	6	63	theme	penta-HILIC	1141:1151	arg1	column					1153:1158	The HALO® penta-HILIC column	1131:1158	The HALO® penta-HILIC column	1131:1158	The HALO® penta-HILIC column provided the best separation results, and the ZIC-HILIC column the worst.					
33065988	6	64	theme	best	1173:1176	arg1	results					1189:1195	the best separation results	1169:1195	the best separation results	1169:1195	The HALO® penta-HILIC column provided the best separation results, and the ZIC-HILIC column the worst.					
33065988	2	65	attach	attached	280:287	arg2	glycans					272:278	chromatographically separating glycans	241:278	chromatographically separating glycans attached to tryptic peptides	241:307	However, chromatographically separating glycans attached to tryptic peptides enables their site-specific characterization.					
33065988	2	65	attach	attached	280:287	arg1	peptides					300:307	tryptic peptides	292:307	tryptic peptides	292:307	However, chromatographically separating glycans attached to tryptic peptides enables their site-specific characterization.					
33065988	6	66	theme	HALO®	1135:1139	arg1	column					1153:1158	The HALO® penta-HILIC column	1131:1158	The HALO® penta-HILIC column	1131:1158	The HALO® penta-HILIC column provided the best separation results, and the ZIC-HILIC column the worst.					
33065988	5	67	theme	charged	1062:1068	arg1	group					1094:1098	the negatively charged sulfobetaine functional group	1047:1098	the negatively charged sulfobetaine functional group	1047:1098	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	6	68	theme	ZIC-HILIC	1206:1214	arg1	column					1216:1221	the ZIC-HILIC column	1202:1221	the ZIC-HILIC column	1202:1221	The HALO® penta-HILIC column provided the best separation results, and the ZIC-HILIC column the worst.					
33065988	1	69	theme	Protein	133:139	arg1	analysis					155:162	Protein glycosylation analysis	133:162	Protein glycosylation analysis	133:162	Protein glycosylation analysis is challenging due to the structural variety of complex conjugates.					
33065988	4	70	theme	hybrid	641:646	arg1	Amide					654:658	Glycan ethylene bridged hybrid (BEH) Amide	617:658	Glycan ethylene bridged hybrid (BEH) Amide	617:658	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33065988	5	71	theme	sulfobetaine	1070:1081	arg1	group					1094:1098	the negatively charged sulfobetaine functional group	1047:1098	the negatively charged sulfobetaine functional group	1047:1098	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	8	72	theme	isomers	1461:1467	arg1	characterization					1417:1432	the comprehensive characterization	1399:1432	the comprehensive characterization of glycoproteins and their isomers	1399:1467	Therefore, HILIC is a useful tool for the comprehensive characterization of glycoproteins and their isomers.					
33065988	1	73	theme	glycosylation	141:153	arg1	analysis					155:162	Protein glycosylation analysis	133:162	Protein glycosylation analysis	133:162	Protein glycosylation analysis is challenging due to the structural variety of complex conjugates.					
33065988	0	74	from	Isomers	124:130	arg1	Separation					55:64	the Separation	51:64	the Separation of Hemopexin and Immunoglobulin G Glycopeptides	51:112	Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.					
33065988	5	75	theme	retention	788:796	arg1	time					798:801	The retention time	784:801	The retention time	784:801	The retention time increased with the polarity of the glycans attached to the same peptide backbone in all HILIC columns tested in this study, except for the ZIC-HILIC column when adding sialic acid to the glycan moiety, which caused electrostatic repulsion with the negatively charged sulfobetaine functional group, thereby decreasing retention.					
33065988	2	76	theme	separating	261:270	arg1	glycans					272:278	chromatographically separating glycans	241:278	chromatographically separating glycans attached to tryptic peptides	241:307	However, chromatographically separating glycans attached to tryptic peptides enables their site-specific characterization.					
33065988	7	77	theme	HILIC	1277:1281	arg1	columns					1283:1289	these HILIC columns	1271:1289	these HILIC columns	1271:1289	Moreover, we showed the potential of these HILIC columns for the isomeric separation of fucosylated and sialylated glycoforms.					
33065988	6	78	theme	separation	1178:1187	arg1	results					1189:1195	the best separation results	1169:1195	the best separation results	1169:1195	The HALO® penta-HILIC column provided the best separation results, and the ZIC-HILIC column the worst.					
33065988	4	79	theme	BEH	649:651	arg1	Amide					654:658	Glycan ethylene bridged hybrid (BEH) Amide	617:658	Glycan ethylene bridged hybrid (BEH) Amide	617:658	Three different HILIC stationary phases, i.e., HALO® penta-HILIC, Glycan ethylene bridged hybrid (BEH) Amide, and ZIC-HILIC, were compared in the separation of complex N-glycopeptides of hemopexin and Immunoglobulin G glycoproteins.					
33234415	8	0	with	chromatography	1585:1598	arg1	detection					1626:1634	fluorescence detection	1613:1634	fluorescence detection	1613:1634	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	0	1	theme	original	199:206	arg1	Eprex®					226:231	original epoetin alfa drug Eprex®	199:231	original epoetin alfa drug Eprex®	199:231	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	8	2	theme	mass	1527:1530	arg1	MS					1546:1547	MS	1546:1547	MS	1546:1547	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	2	theme	mass	1527:1530	arg1	spectrometry					1532:1543	MALDI-TOF mass spectrometry	1517:1543	MALDI-TOF mass spectrometry (MS)	1517:1548	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	3	3	theme	particular	534:543	arg1	importance					545:554	particular importance	534:554	particular importance	534:554	Differences in N-glycosylation profiles are of particular importance for rEPOs, because they can drastically impact the half-life in circulation and activity.					
33234415	0	4	theme	electrophoretic	74:88	arg1	methods					90:96	electrophoretic methods	74:96	electrophoretic methods used for doping control analysis and specific N-glycan analysis	74:160	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	5	5	dep	evaluated	843:851	arg1	Jimaixin™					854:862	Jimaixin™	854:862	Jimaixin™	854:862	In this study three not well characterized biosimilars were evaluated (Jimaixin™ authorized in China, and Hemax® and Epotin™ authorized in Algeria).					
33234415	5	5	dep	evaluated	843:851	arg1	Hemax®					889:894	Hemax®	889:894	Hemax®	889:894	In this study three not well characterized biosimilars were evaluated (Jimaixin™ authorized in China, and Hemax® and Epotin™ authorized in Algeria).					
33234415	5	5	dep	evaluated	843:851	arg1	Epotin™					900:906	Epotin™	900:906	Epotin™	900:906	In this study three not well characterized biosimilars were evaluated (Jimaixin™ authorized in China, and Hemax® and Epotin™ authorized in Algeria).					
33234415	8	6	theme	complementary	1491:1503	arg1	approaches					1505:1514	two complementary approaches	1487:1514	two complementary approaches	1487:1514	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	0	7	theme	alfa	216:219	arg1	Eprex®					226:231	original epoetin alfa drug Eprex®	199:231	original epoetin alfa drug Eprex®	199:231	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	6	8	dep	charge	1081:1086	arg1	isolectric					1089:1098	isolectric	1089:1098	isolectric	1089:1098	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	6	8	dep	charge	1081:1086	arg1	IEF-PAGE					1110:1117	IEF-PAGE	1110:1117	IEF-PAGE	1110:1117	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	6	9	theme	EPO	988:990	arg1	profiles					992:999	their EPO profiles	982:999	their EPO profiles	982:999	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	8	10	theme	MALDI-TOF	1517:1525	arg1	MS					1546:1547	MS	1546:1547	MS	1546:1547	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	10	theme	MALDI-TOF	1517:1525	arg1	spectrometry					1532:1543	MALDI-TOF mass spectrometry	1517:1543	MALDI-TOF mass spectrometry (MS)	1517:1548	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	0	11	theme	epoetin	208:214	arg1	Eprex®					226:231	original epoetin alfa drug Eprex®	199:231	original epoetin alfa drug Eprex®	199:231	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	2	12	from	differences	363:373	arg1	process					382:388	the process	378:388	the process of production	378:402	However differences in the process of production can result in small structural differences when compared to the reference product.					
33234415	6	13	theme	EPO	1168:1170	arg1	methods					1038:1044	the antidoping methods	1023:1044	the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection)	1023:1187	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	6	13	theme	EPO	1168:1170	arg1	immunodetection					1172:1186	specific EPO immunodetection	1159:1186	specific EPO immunodetection	1159:1186	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	9	14	theme	complex	1759:1765	arg1	forms					1767:1771	less complex forms	1754:1771	less complex forms	1754:1771	All three biosimilars presented a significant decrease in the major glycan forms of Eprex® along with an increase in less complex forms.					
33234415	7	15	theme	basic	1253:1257	arg1	isoforms					1259:1266	more basic isoforms	1248:1266	more basic isoforms for Epotin™ and Jimaixin™	1248:1292	Compared to the original epoetin alfa Eprex®, it revealed more basic isoforms for Epotin™ and Jimaixin™ after IEF-PAGE and a slightly lower molecular weight after SDS-PAGE in particular for Hemax®.					
33234415	0	16	theme	control	114:120	arg1	analysis					122:129	doping control analysis	107:129	doping control analysis	107:129	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	5	17	dep	well	807:810	arg1	not					803:805	not	803:805	not	803:805	In this study three not well characterized biosimilars were evaluated (Jimaixin™ authorized in China, and Hemax® and Epotin™ authorized in Algeria).					
33234415	10	18	gly	sialylated	1835:1844	arg1	forms					1846:1850	fully sialylated forms	1829:1850	fully sialylated forms	1829:1850	Jimaixin™ and Epotin™ presented also a lower amount of fully sialylated forms.					
33234415	6	19	theme	specific	1159:1166	arg1	methods					1038:1044	the antidoping methods	1023:1044	the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection)	1023:1187	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	6	19	theme	specific	1159:1166	arg1	immunodetection					1172:1186	specific EPO immunodetection	1159:1186	specific EPO immunodetection	1159:1186	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	0	20	theme	doping	107:112	arg1	analysis					122:129	doping control analysis	107:129	doping control analysis	107:129	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	0	21	theme	drug	221:224	arg1	Eprex®					226:231	original epoetin alfa drug Eprex®	199:231	original epoetin alfa drug Eprex®	199:231	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	0	22	from	Eprex®	226:231	arg1	differences					182:192	structural differences	171:192	structural differences from original epoetin alfa drug Eprex®	171:231	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	2	23	theme	reference	468:476	arg1	product					478:484	the reference product	464:484	the reference product	464:484	However differences in the process of production can result in small structural differences when compared to the reference product.					
33234415	4	24	attach	presence	736:743	arg2	rEPO					750:753	a rEPO	748:753	a rEPO	748:753	Changes of structure can also impact electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample.					
33234415	4	24	attach	presence	736:743	arg1	sample					775:780	a doping control sample	758:780	a doping control sample	758:780	Changes of structure can also impact electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample.					
33234415	7	25	theme	original	1206:1213	arg1	Eprex®					1228:1233	the original epoetin alfa Eprex®	1202:1233	the original epoetin alfa Eprex®	1202:1233	Compared to the original epoetin alfa Eprex®, it revealed more basic isoforms for Epotin™ and Jimaixin™ after IEF-PAGE and a slightly lower molecular weight after SDS-PAGE in particular for Hemax®.					
33234415	4	26	theme	doping	760:765	arg1	sample					775:780	a doping control sample	758:780	a doping control sample	758:780	Changes of structure can also impact electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample.					
33234415	11	27	theme	acid	1913:1916	arg1	residues					1918:1925	sialic acid residues	1906:1925	sialic acid residues	1906:1925	HILIC method also showed that O-acetylation level of sialic acid residues might vary from one rEPO to the other.					
33234415	11	28	theme	O-acetylation	1883:1895	arg1	level					1897:1901	O-acetylation level	1883:1901	O-acetylation level of sialic acid residues	1883:1925	HILIC method also showed that O-acetylation level of sialic acid residues might vary from one rEPO to the other.					
33234415	10	29	theme	lower	1813:1817	arg1	amount					1819:1824	a lower amount	1811:1824	a lower amount of fully sialylated forms	1811:1850	Jimaixin™ and Epotin™ presented also a lower amount of fully sialylated forms.					
33234415	10	29	theme	lower	1813:1817	arg1	forms					1846:1850	fully sialylated forms	1829:1850	fully sialylated forms	1829:1850	Jimaixin™ and Epotin™ presented also a lower amount of fully sialylated forms.					
33234415	9	30	theme	significant	1671:1681	arg1	decrease					1683:1690	a significant decrease	1669:1690	a significant decrease in the major glycan forms of Eprex®	1669:1726	All three biosimilars presented a significant decrease in the major glycan forms of Eprex® along with an increase in less complex forms.					
33234415	7	31	from	SDS-PAGE	1353:1360	arg1	particular					1365:1374	particular	1365:1374	particular	1365:1374	Compared to the original epoetin alfa Eprex®, it revealed more basic isoforms for Epotin™ and Jimaixin™ after IEF-PAGE and a slightly lower molecular weight after SDS-PAGE in particular for Hemax®.					
33234415	2	32	theme	production	393:402	arg1	process					382:388	the process	378:388	the process of production	378:402	However differences in the process of production can result in small structural differences when compared to the reference product.					
33234415	0	33	theme	biosimilars	29:39	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis	0:160	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	1	34	theme	epoetin	300:306	arg1	drugs					308:312	epoetin drugs	300:312	epoetin drugs developed after the first patents ended	300:352	Recombinant human erythropoietin (rEPO) biosimilars are copies of epoetin drugs developed after the first patents ended.					
33234415	6	35	used	used	959:962	arg2	products					941:948	these products	935:948	these products	935:948	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	8	36	with	spectrometry	1532:1543	arg1	detection					1626:1634	fluorescence detection	1613:1634	fluorescence detection	1613:1634	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	0	37	theme	erythropoietin	14:27	arg1	Jimaixin™					61:69	Jimaixin™	61:69	Jimaixin™	61:69	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	0	37	theme	erythropoietin	14:27	arg1	biosimilars					29:39	erythropoietin biosimilars	14:39	erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™	14:69	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	0	37	theme	erythropoietin	14:27	arg1	Hemax®					50:55	Hemax®	50:55	Hemax®	50:55	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	0	37	theme	erythropoietin	14:27	arg1	Epotin™					41:47	Epotin™	41:47	Epotin™	41:47	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	1	38	theme	drugs	308:312	arg1	biosimilars					274:284	Recombinant human erythropoietin (rEPO) biosimilars	234:284	Recombinant human erythropoietin (rEPO) biosimilars	234:284	Recombinant human erythropoietin (rEPO) biosimilars are copies of epoetin drugs developed after the first patents ended.					
33234415	1	38	theme	drugs	308:312	arg1	copies					290:295	copies	290:295	copies of epoetin drugs developed after the first patents ended	290:352	Recombinant human erythropoietin (rEPO) biosimilars are copies of epoetin drugs developed after the first patents ended.					
33234415	9	39	theme	glycan	1705:1710	arg1	forms					1712:1716	the major glycan forms	1695:1716	the major glycan forms of Eprex®	1695:1726	All three biosimilars presented a significant decrease in the major glycan forms of Eprex® along with an increase in less complex forms.					
33234415	4	40	theme	control	767:773	arg1	sample					775:780	a doping control sample	758:780	a doping control sample	758:780	Changes of structure can also impact electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample.					
33234415	6	41	theme	electrophoretic	1047:1061	arg1	methods					1038:1044	the antidoping methods	1023:1044	the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection)	1023:1187	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	6	41	theme	electrophoretic	1047:1061	arg1	separation					1063:1072	electrophoretic separation	1047:1072	electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE)	1047:1153	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	9	42	from	decrease	1683:1690	arg1	forms					1712:1716	the major glycan forms	1695:1716	the major glycan forms of Eprex®	1695:1726	All three biosimilars presented a significant decrease in the major glycan forms of Eprex® along with an increase in less complex forms.					
33234415	0	43	theme	N-glycan	144:151	arg1	analysis					153:160	specific N-glycan analysis	135:160	specific N-glycan analysis	135:160	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	4	44	theme	electrophoretic	683:697	arg1	profiles					699:706	electrophoretic profiles	683:706	electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample	683:780	Changes of structure can also impact electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample.					
33234415	2	45	theme	structural	424:433	arg1	differences					435:445	small structural differences	418:445	small structural differences	418:445	However differences in the process of production can result in small structural differences when compared to the reference product.					
33234415	11	46	theme	HILIC	1853:1857	arg1	method					1859:1864	HILIC method	1853:1864	HILIC method	1853:1864	HILIC method also showed that O-acetylation level of sialic acid residues might vary from one rEPO to the other.					
33234415	8	47	theme	interaction	1566:1576	arg1	HILIC					1601:1605	HILIC	1601:1605	HILIC	1601:1605	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	47	theme	interaction	1566:1576	arg1	chromatography					1585:1598	hydrophilic interaction liquid chromatography	1554:1598	hydrophilic interaction liquid chromatography (HILIC)	1554:1606	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	0	48	theme	specific	135:142	arg1	analysis					153:160	specific N-glycan analysis	135:160	specific N-glycan analysis	135:160	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	8	49	theme	EPO	1443:1445	arg1	N-glycans					1456:1464	EPO specific N-glycans	1443:1464	EPO specific N-glycans	1443:1464	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	1	50	theme	Recombinant	234:244	arg1	rEPO					268:271	rEPO	268:271	rEPO	268:271	Recombinant human erythropoietin (rEPO) biosimilars are copies of epoetin drugs developed after the first patents ended.					
33234415	1	50	theme	Recombinant	234:244	arg1	erythropoietin					252:265	Recombinant human erythropoietin	234:265	Recombinant human erythropoietin (rEPO) biosimilars	234:284	Recombinant human erythropoietin (rEPO) biosimilars are copies of epoetin drugs developed after the first patents ended.					
33234415	8	51	dep	understand	1398:1407	arg1	HILIC					1601:1605	HILIC	1601:1605	HILIC	1601:1605	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	51	dep	understand	1398:1407	arg1	chromatography					1585:1598	hydrophilic interaction liquid chromatography	1554:1598	hydrophilic interaction liquid chromatography (HILIC)	1554:1606	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	51	dep	understand	1398:1407	arg1	MS					1546:1547	MS	1546:1547	MS	1546:1547	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	51	dep	understand	1398:1407	arg1	spectrometry					1532:1543	MALDI-TOF mass spectrometry	1517:1543	MALDI-TOF mass spectrometry (MS)	1517:1548	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	52	theme	hydrophilic	1554:1564	arg1	HILIC					1601:1605	HILIC	1601:1605	HILIC	1601:1605	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	52	theme	hydrophilic	1554:1564	arg1	chromatography					1585:1598	hydrophilic interaction liquid chromatography	1554:1598	hydrophilic interaction liquid chromatography (HILIC)	1554:1606	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	9	53	theme	Eprex®	1721:1726	arg1	forms					1712:1716	the major glycan forms	1695:1716	the major glycan forms of Eprex®	1695:1726	All three biosimilars presented a significant decrease in the major glycan forms of Eprex® along with an increase in less complex forms.					
33234415	10	54	theme	sialylated	1835:1844	arg1	forms					1846:1850	fully sialylated forms	1829:1850	fully sialylated forms	1829:1850	Jimaixin™ and Epotin™ presented also a lower amount of fully sialylated forms.					
33234415	4	55	used	used	717:720	arg2	profiles					699:706	electrophoretic profiles	683:706	electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample	683:780	Changes of structure can also impact electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample.					
33234415	6	56	theme	molecular	1127:1135	arg1	SDS-PAGE					1145:1152	SDS-PAGE	1145:1152	SDS-PAGE	1145:1152	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	6	56	theme	molecular	1127:1135	arg1	weight					1137:1142	the molecular weight	1123:1142	the molecular weight (SDS-PAGE)	1123:1153	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	5	57	theme	characterized	812:824	arg1	biosimilars					826:836	three not well characterized biosimilars	797:836	three not well characterized biosimilars	797:836	In this study three not well characterized biosimilars were evaluated (Jimaixin™ authorized in China, and Hemax® and Epotin™ authorized in Algeria).					
33234415	1	58	theme	first	334:338	arg1	patents					340:346	the first patents	330:346	the first patents ended	330:352	Recombinant human erythropoietin (rEPO) biosimilars are copies of epoetin drugs developed after the first patents ended.					
33234415	9	59	theme	major	1699:1703	arg1	forms					1712:1716	the major glycan forms	1695:1716	the major glycan forms of Eprex®	1695:1726	All three biosimilars presented a significant decrease in the major glycan forms of Eprex® along with an increase in less complex forms.					
33234415	3	60	from	Differences	487:497	arg1	profiles					518:525	N-glycosylation profiles	502:525	N-glycosylation profiles	502:525	Differences in N-glycosylation profiles are of particular importance for rEPOs, because they can drastically impact the half-life in circulation and activity.					
33234415	10	61	theme	forms	1846:1850	arg1	amount					1819:1824	a lower amount	1811:1824	a lower amount of fully sialylated forms	1811:1850	Jimaixin™ and Epotin™ presented also a lower amount of fully sialylated forms.					
33234415	10	61	theme	forms	1846:1850	arg1	forms					1846:1850	fully sialylated forms	1829:1850	fully sialylated forms	1829:1850	Jimaixin™ and Epotin™ presented also a lower amount of fully sialylated forms.					
33234415	8	62	theme	liquid	1578:1583	arg1	HILIC					1601:1605	HILIC	1601:1605	HILIC	1601:1605	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	62	theme	liquid	1578:1583	arg1	chromatography					1585:1598	hydrophilic interaction liquid chromatography	1554:1598	hydrophilic interaction liquid chromatography (HILIC)	1554:1606	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	63	theme	fluorescence	1613:1624	arg1	detection					1626:1634	fluorescence detection	1613:1634	fluorescence detection	1613:1634	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	8	64	theme	specific	1447:1454	arg1	N-glycans					1456:1464	EPO specific N-glycans	1443:1464	EPO specific N-glycans	1443:1464	To better understand the reason for these differences, EPO specific N-glycans were evaluated using two complementary approaches: MALDI-TOF mass spectrometry (MS) and hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection.					
33234415	0	65	dep	biosimilars	29:39	arg1	Jimaixin™					61:69	Jimaixin™	61:69	Jimaixin™	61:69	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	0	65	dep	biosimilars	29:39	arg1	biosimilars					29:39	erythropoietin biosimilars	14:39	erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™	14:69	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	0	65	dep	biosimilars	29:39	arg1	Hemax®					50:55	Hemax®	50:55	Hemax®	50:55	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	0	65	dep	biosimilars	29:39	arg1	Epotin™					41:47	Epotin™	41:47	Epotin™	41:47	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	3	66	theme	N-glycosylation	502:516	arg1	profiles					518:525	N-glycosylation profiles	502:525	N-glycosylation profiles	502:525	Differences in N-glycosylation profiles are of particular importance for rEPOs, because they can drastically impact the half-life in circulation and activity.					
33234415	3	67	from	impact	596:601	arg1	activity					636:643	activity	636:643	activity	636:643	Differences in N-glycosylation profiles are of particular importance for rEPOs, because they can drastically impact the half-life in circulation and activity.					
33234415	3	67	from	impact	596:601	arg1	circulation					620:630	circulation	620:630	circulation	620:630	Differences in N-glycosylation profiles are of particular importance for rEPOs, because they can drastically impact the half-life in circulation and activity.					
33234415	1	68	theme	human	246:250	arg1	rEPO					268:271	rEPO	268:271	rEPO	268:271	Recombinant human erythropoietin (rEPO) biosimilars are copies of epoetin drugs developed after the first patents ended.					
33234415	1	68	theme	human	246:250	arg1	erythropoietin					252:265	Recombinant human erythropoietin	234:265	Recombinant human erythropoietin (rEPO) biosimilars	234:284	Recombinant human erythropoietin (rEPO) biosimilars are copies of epoetin drugs developed after the first patents ended.					
33234415	0	69	theme	structural	171:180	arg1	differences					182:192	structural differences	171:192	structural differences from original epoetin alfa drug Eprex®	171:231	Evaluation of erythropoietin biosimilars Epotin™, Hemax® and Jimaixin™ by electrophoretic methods used for doping control analysis and specific N-glycan analysis revealed structural differences from original epoetin alfa drug Eprex®.					
33234415	9	70	from	increase	1742:1749	arg1	forms					1767:1771	less complex forms	1754:1771	less complex forms	1754:1771	All three biosimilars presented a significant decrease in the major glycan forms of Eprex® along with an increase in less complex forms.					
33234415	4	71	theme	rEPO	750:753	arg1	presence					736:743	the presence	732:743	the presence of a rEPO in a doping control sample	732:780	Changes of structure can also impact electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample.					
33234415	1	72	theme	erythropoietin	252:265	arg1	biosimilars					274:284	Recombinant human erythropoietin (rEPO) biosimilars	234:284	Recombinant human erythropoietin (rEPO) biosimilars	234:284	Recombinant human erythropoietin (rEPO) biosimilars are copies of epoetin drugs developed after the first patents ended.					
33234415	1	72	theme	erythropoietin	252:265	arg1	copies					290:295	copies	290:295	copies of epoetin drugs developed after the first patents ended	290:352	Recombinant human erythropoietin (rEPO) biosimilars are copies of epoetin drugs developed after the first patents ended.					
33234415	7	73	theme	epoetin	1215:1221	arg1	Eprex®					1228:1233	the original epoetin alfa Eprex®	1202:1233	the original epoetin alfa Eprex®	1202:1233	Compared to the original epoetin alfa Eprex®, it revealed more basic isoforms for Epotin™ and Jimaixin™ after IEF-PAGE and a slightly lower molecular weight after SDS-PAGE in particular for Hemax®.					
33234415	2	74	theme	small	418:422	arg1	differences					435:445	small structural differences	418:445	small structural differences	418:445	However differences in the process of production can result in small structural differences when compared to the reference product.					
33234415	7	75	theme	lower	1324:1328	arg1	weight					1340:1345	a slightly lower molecular weight	1313:1345	a slightly lower molecular weight	1313:1345	Compared to the original epoetin alfa Eprex®, it revealed more basic isoforms for Epotin™ and Jimaixin™ after IEF-PAGE and a slightly lower molecular weight after SDS-PAGE in particular for Hemax®.					
33234415	11	76	theme	residues	1918:1925	arg1	level					1897:1901	O-acetylation level	1883:1901	O-acetylation level of sialic acid residues	1883:1925	HILIC method also showed that O-acetylation level of sialic acid residues might vary from one rEPO to the other.					
33234415	11	77	theme	sialic	1906:1911	arg1	residues					1918:1925	sialic acid residues	1906:1925	sialic acid residues	1906:1925	HILIC method also showed that O-acetylation level of sialic acid residues might vary from one rEPO to the other.					
33234415	4	78	theme	structure	657:665	arg1	Changes					646:652	Changes	646:652	Changes of structure	646:665	Changes of structure can also impact electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample.					
33234415	7	79	theme	alfa	1223:1226	arg1	Eprex®					1228:1233	the original epoetin alfa Eprex®	1202:1233	the original epoetin alfa Eprex®	1202:1233	Compared to the original epoetin alfa Eprex®, it revealed more basic isoforms for Epotin™ and Jimaixin™ after IEF-PAGE and a slightly lower molecular weight after SDS-PAGE in particular for Hemax®.					
33234415	7	80	theme	molecular	1330:1338	arg1	weight					1340:1345	a slightly lower molecular weight	1313:1345	a slightly lower molecular weight	1313:1345	Compared to the original epoetin alfa Eprex®, it revealed more basic isoforms for Epotin™ and Jimaixin™ after IEF-PAGE and a slightly lower molecular weight after SDS-PAGE in particular for Hemax®.					
33234415	6	81	theme	antidoping	1027:1036	arg1	methods					1038:1044	the antidoping methods	1023:1044	the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection)	1023:1187	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	6	81	theme	antidoping	1027:1036	arg1	separation					1063:1072	electrophoretic separation	1047:1072	electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE)	1047:1153	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	6	81	theme	antidoping	1027:1036	arg1	immunodetection					1172:1186	specific EPO immunodetection	1159:1186	specific EPO immunodetection	1159:1186	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	4	82	from	presence	736:743	arg1	sample					775:780	a doping control sample	758:780	a doping control sample	758:780	Changes of structure can also impact electrophoretic profiles that are used to reveal the presence of a rEPO in a doping control sample.					
33234415	6	83	dep	methods	1038:1044	arg1	methods					1038:1044	the antidoping methods	1023:1044	the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection)	1023:1187	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	6	83	dep	methods	1038:1044	arg1	separation					1063:1072	electrophoretic separation	1047:1072	electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE)	1047:1153	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
33234415	6	83	dep	methods	1038:1044	arg1	immunodetection					1172:1186	specific EPO immunodetection	1159:1186	specific EPO immunodetection	1159:1186	As these products could be used for doping, first their EPO profiles were determined using the antidoping methods (electrophoretic separation by the charge (isolectric focusing, IEF-PAGE) or the molecular weight (SDS-PAGE) and specific EPO immunodetection).					
32206767	1	0	theme	structural	192:201	arg1	studies					218:224	structural and functional studies	192:224	structural and functional studies of carbohydrate-active enzymes	192:255	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	4	1	theme	protected	803:811	arg1	tetrasaccharide					813:827	a selectively protected tetrasaccharide	789:827	a selectively protected tetrasaccharide	789:827	The resulting S-linked disaccharide was subsequently employed in a series of glycosylation reactions to obtain a selectively protected tetrasaccharide.					
32206767	1	2	theme	enzyme	292:297	arg1	inhibitors					299:308	enzyme inhibitors	292:308	enzyme inhibitors	292:308	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	1	2	theme	enzyme	292:297	arg1	they					261:264	they	261:264	they	261:264	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	2	3	theme	enzymes	511:517	arg1	study					488:492	the study	484:492	the study of AXs degrading enzymes	484:517	In this context, this work focuses on the introduction of an S-linkage into arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes.					
32206767	5	4	theme	glycosylation	903:915	arg1	reactions					917:925	glycosylation reactions	903:925	glycosylation reactions	903:925	This could be further elaborated through chemoselective deprotection and glycosylation reactions to introduce branching l-arabinofuranosides.					
32206767	1	5	theme	important	84:92	arg1	S-Glycosides					67:78	S-Glycosides	67:78	S-Glycosides	67:78	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	1	5	theme	important	84:92	arg1	tools					94:98	important tools	84:98	important tools for the elucidation of specific protein-carbohydrate interactions	84:164	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	4	6	theme	resulting	682:690	arg1	disaccharide					701:712	The resulting S-linked disaccharide	678:712	The resulting S-linked disaccharide	678:712	The resulting S-linked disaccharide was subsequently employed in a series of glycosylation reactions to obtain a selectively protected tetrasaccharide.					
32206767	2	7	theme	degrading	501:509	arg1	enzymes					511:517	AXs degrading enzymes	497:517	AXs degrading enzymes	497:517	In this context, this work focuses on the introduction of an S-linkage into arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes.					
32206767	1	8	theme	functional	207:216	arg1	studies					218:224	structural and functional studies	192:224	structural and functional studies of carbohydrate-active enzymes	192:255	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	2	9	theme	small	444:448	arg1	collection					450:459	a small collection	442:459	a small collection of synthetic tools for the study of AXs degrading enzymes	442:517	In this context, this work focuses on the introduction of an S-linkage into arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes.					
32206767	3	10	theme	S-glycosidic	561:572	arg1	linkage					574:580	the S-glycosidic linkage	557:580	the S-glycosidic linkage	557:580	The key step for the introduction of the S-glycosidic linkage involved anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate.					
32206767	2	11	theme	arabinoxylan	387:398	arg1	AXs					418:420	AXs	418:420	AXs	418:420	In this context, this work focuses on the introduction of an S-linkage into arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes.					
32206767	2	11	theme	arabinoxylan	387:398	arg1	oligosaccharides					400:415	arabinoxylan oligosaccharides	387:415	arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes	387:517	In this context, this work focuses on the introduction of an S-linkage into arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes.					
32206767	5	12	theme	chemoselective	871:884	arg1	deprotection					886:897	chemoselective deprotection	871:897	chemoselective deprotection	871:897	This could be further elaborated through chemoselective deprotection and glycosylation reactions to introduce branching l-arabinofuranosides.					
32206767	0	13	link	S-linked	27:34	arg1	oligosaccharides					49:64	S-linked arabinoxylan oligosaccharides	27:64	S-linked arabinoxylan oligosaccharides	27:64	S-Glycosides: synthesis of S-linked arabinoxylan oligosaccharides.					
32206767	1	14	theme	carbohydrate-active	229:247	arg1	enzymes					249:255	carbohydrate-active enzymes	229:255	carbohydrate-active enzymes	229:255	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	4	15	theme	reactions	769:777	arg1	series					745:750	a series	743:750	a series of glycosylation reactions	743:777	The resulting S-linked disaccharide was subsequently employed in a series of glycosylation reactions to obtain a selectively protected tetrasaccharide.					
32206767	3	16	theme	key	524:526	arg1	step					528:531	The key step	520:531	The key step for the introduction of the S-glycosidic linkage	520:580	The key step for the introduction of the S-glycosidic linkage involved anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate.					
32206767	3	17	theme	triflate	668:675	arg1	S-alkylation					606:617	anomeric thiol S-alkylation	591:617	anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate	591:675	The key step for the introduction of the S-glycosidic linkage involved anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate.					
32206767	1	18	theme	enzymes	249:255	arg1	studies					218:224	structural and functional studies	192:224	structural and functional studies of carbohydrate-active enzymes	192:255	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	0	19	theme	arabinoxylan	36:47	arg1	oligosaccharides					49:64	S-linked arabinoxylan oligosaccharides	27:64	S-linked arabinoxylan oligosaccharides	27:64	S-Glycosides: synthesis of S-linked arabinoxylan oligosaccharides.					
32206767	3	20	theme	anomeric	591:598	arg1	S-alkylation					606:617	anomeric thiol S-alkylation	591:617	anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate	591:675	The key step for the introduction of the S-glycosidic linkage involved anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate.					
32206767	4	21	theme	S-linked	692:699	arg1	disaccharide					701:712	The resulting S-linked disaccharide	678:712	The resulting S-linked disaccharide	678:712	The resulting S-linked disaccharide was subsequently employed in a series of glycosylation reactions to obtain a selectively protected tetrasaccharide.					
32206767	2	22	theme	tools	474:478	arg1	collection					450:459	a small collection	442:459	a small collection of synthetic tools for the study of AXs degrading enzymes	442:517	In this context, this work focuses on the introduction of an S-linkage into arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes.					
32206767	0	23	theme	S-linked	27:34	arg1	oligosaccharides					49:64	S-linked arabinoxylan oligosaccharides	27:64	S-linked arabinoxylan oligosaccharides	27:64	S-Glycosides: synthesis of S-linked arabinoxylan oligosaccharides.					
32206767	3	24	theme	protected	638:646	arg1	triflate					668:675	an orthogonally protected l-arabinopyranoside triflate	622:675	an orthogonally protected l-arabinopyranoside triflate	622:675	The key step for the introduction of the S-glycosidic linkage involved anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate.					
32206767	3	25	theme	thiol	600:604	arg1	S-alkylation					606:617	anomeric thiol S-alkylation	591:617	anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate	591:675	The key step for the introduction of the S-glycosidic linkage involved anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate.					
32206767	2	26	theme	synthetic	464:472	arg1	tools					474:478	synthetic tools	464:478	synthetic tools for the study of AXs degrading enzymes	464:517	In this context, this work focuses on the introduction of an S-linkage into arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes.					
32206767	3	27	theme	l-arabinopyranoside	648:666	arg1	triflate					668:675	an orthogonally protected l-arabinopyranoside triflate	622:675	an orthogonally protected l-arabinopyranoside triflate	622:675	The key step for the introduction of the S-glycosidic linkage involved anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate.					
32206767	3	28	theme	linkage	574:580	arg1	introduction					541:552	the introduction	537:552	the introduction of the S-glycosidic linkage	537:580	The key step for the introduction of the S-glycosidic linkage involved anomeric thiol S-alkylation of an orthogonally protected l-arabinopyranoside triflate.					
32206767	1	29	theme	specific	123:130	arg1	interactions					153:164	specific protein-carbohydrate interactions	123:164	specific protein-carbohydrate interactions	123:164	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	0	30	theme	oligosaccharides	49:64	arg1	synthesis					14:22	synthesis	14:22	synthesis of S-linked arabinoxylan oligosaccharides	14:64	S-Glycosides: synthesis of S-linked arabinoxylan oligosaccharides.					
32206767	1	31	theme	protein-carbohydrate	132:151	arg1	interactions					153:164	specific protein-carbohydrate interactions	123:164	specific protein-carbohydrate interactions	123:164	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	4	32	link	S-linked	692:699	arg1	disaccharide					701:712	The resulting S-linked disaccharide	678:712	The resulting S-linked disaccharide	678:712	The resulting S-linked disaccharide was subsequently employed in a series of glycosylation reactions to obtain a selectively protected tetrasaccharide.					
32206767	1	33	theme	interactions	153:164	arg1	elucidation					108:118	the elucidation	104:118	the elucidation of specific protein-carbohydrate interactions	104:164	S-Glycosides are important tools for the elucidation of specific protein-carbohydrate interactions and can significantly aid structural and functional studies of carbohydrate-active enzymes, as they are often inert or act as enzyme inhibitors.					
32206767	0	34	dep	synthesis	14:22	arg1	S-Glycosides					0:11	S-Glycosides	0:11	S-Glycosides	0:11	S-Glycosides: synthesis of S-linked arabinoxylan oligosaccharides.					
32206767	4	35	theme	glycosylation	755:767	arg1	reactions					769:777	glycosylation reactions	755:777	glycosylation reactions	755:777	The resulting S-linked disaccharide was subsequently employed in a series of glycosylation reactions to obtain a selectively protected tetrasaccharide.					
32206767	2	36	theme	AXs	497:499	arg1	enzymes					511:517	AXs degrading enzymes	497:517	AXs degrading enzymes	497:517	In this context, this work focuses on the introduction of an S-linkage into arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes.					
32206767	5	37	theme	branching	940:948	arg1	l-arabinofuranosides					950:969	branching l-arabinofuranosides	940:969	branching l-arabinofuranosides	940:969	This could be further elaborated through chemoselective deprotection and glycosylation reactions to introduce branching l-arabinofuranosides.					
32206767	2	38	theme	S-linkage	372:380	arg1	introduction					353:364	the introduction	349:364	the introduction of an S-linkage into arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes	349:517	In this context, this work focuses on the introduction of an S-linkage into arabinoxylan oligosaccharides (AXs) in order to obtain a small collection of synthetic tools for the study of AXs degrading enzymes.					
34485242	0	0	theme	Interaction	91:101	arg1	Materials					118:126	the Dual-Functional Hydrophilic Interaction Chromatography Materials	59:126	the Dual-Functional Hydrophilic Interaction Chromatography Materials	59:126	Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials.					
34485242	8	1	theme	HKU1	1005:1008	arg1	S					1010:1010	HKU1 S	1005:1010	HKU1 S	1005:1010	The obtained O-glycosylation dataset will provide valuable insights into the structure of HKU1 S.					
34485242	8	2	theme	obtained	919:926	arg1	dataset					944:950	The obtained O-glycosylation dataset	915:950	The obtained O-glycosylation dataset	915:950	The obtained O-glycosylation dataset will provide valuable insights into the structure of HKU1 S.					
34485242	5	3	gly	O-glycopeptides	566:580	arg2	O-glycopeptides					566:580	O-glycopeptides	566:580	O-glycopeptides	566:580	The enrichment method for O-glycopeptides with HBS was developed and validated using standard proteins.					
34485242	0	4	theme	Hydrophilic	79:89	arg1	Materials					118:126	the Dual-Functional Hydrophilic Interaction Chromatography Materials	59:126	the Dual-Functional Hydrophilic Interaction Chromatography Materials	59:126	Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials.					
34485242	4	5	theme	silica	516:521	arg1	materials					529:537	dual-functional histidine-bonded silica (HBS) materials	483:537	dual-functional histidine-bonded silica (HBS) materials	483:537	Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials.					
34485242	1	6	theme	host	174:177	arg1	cells					179:183	host cells	174:183	host cells	174:183	HKU1 is a human beta coronavirus and infects host cells via highly glycosylated spike protein (S).					
34485242	7	7	theme	O-linked	842:849	arg1	glycans					851:857	the O-linked glycans	838:857	the O-linked glycans	838:857	Moreover, the O-linked glycans and their abundance on each HKU1 S1 site were analyzed.					
34485242	5	8	with	method	555:560	arg1	HBS					587:589	HBS	587:589	HBS	587:589	The enrichment method for O-glycopeptides with HBS was developed and validated using standard proteins.					
34485242	4	9	theme	histidine-bonded	499:514	arg1	HBS					524:526	HBS	524:526	HBS	524:526	Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials.					
34485242	4	9	theme	histidine-bonded	499:514	arg1	silica					516:521	dual-functional histidine-bonded silica	483:521	dual-functional histidine-bonded silica (HBS) materials	483:537	Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials.					
34485242	1	10	gly	glycosylated	196:207	arg1	S					224:224	highly glycosylated spike protein (S)	189:225	highly glycosylated spike protein (S)	189:225	HKU1 is a human beta coronavirus and infects host cells via highly glycosylated spike protein (S).					
34485242	3	11	theme	in-depth	347:354	arg1	understanding					356:368	the in-depth understanding	343:368	the in-depth understanding of its biological functions	343:396	However, little is known about its O-glycosylation, which hinders the in-depth understanding of its biological functions.					
34485242	8	12	theme	S	1010:1010	arg1	structure					992:1000	the structure	988:1000	the structure of HKU1 S	988:1010	The obtained O-glycosylation dataset will provide valuable insights into the structure of HKU1 S.					
34485242	8	13	theme	valuable	965:972	arg1	insights					974:981	valuable insights	965:981	valuable insights into the structure of HKU1 S	965:1010	The obtained O-glycosylation dataset will provide valuable insights into the structure of HKU1 S.					
34485242	7	14	from	glycans	851:857	arg1	site					895:898	each HKU1 S1 site	882:898	each HKU1 S1 site	882:898	Moreover, the O-linked glycans and their abundance on each HKU1 S1 site were analyzed.					
34485242	5	15	theme	standard	625:632	arg1	proteins					634:641	standard proteins	625:641	standard proteins	625:641	The enrichment method for O-glycopeptides with HBS was developed and validated using standard proteins.					
34485242	6	16	theme	S1	696:697	arg1	subunit					699:705	the HKU1 S1 subunit	687:705	the HKU1 S1 subunit	687:705	The application of the developed method to the HKU1 S1 subunit resulted in 46 novel O-glycosylation sites, among which 55.6% were predicted to be exposed on the outer protein surface.					
34485242	7	17	from	abundance	869:877	arg1	site					895:898	each HKU1 S1 site	882:898	each HKU1 S1 site	882:898	Moreover, the O-linked glycans and their abundance on each HKU1 S1 site were analyzed.					
34485242	6	18	theme	HKU1	691:694	arg1	subunit					699:705	the HKU1 S1 subunit	687:705	the HKU1 S1 subunit	687:705	The application of the developed method to the HKU1 S1 subunit resulted in 46 novel O-glycosylation sites, among which 55.6% were predicted to be exposed on the outer protein surface.					
34485242	3	19	theme	biological	377:386	arg1	functions					388:396	its biological functions	373:396	its biological functions	373:396	However, little is known about its O-glycosylation, which hinders the in-depth understanding of its biological functions.					
34485242	7	20	theme	S1	892:893	arg1	site					895:898	each HKU1 S1 site	882:898	each HKU1 S1 site	882:898	Moreover, the O-linked glycans and their abundance on each HKU1 S1 site were analyzed.					
34485242	1	21	theme	glycosylated	196:207	arg1	S					224:224	highly glycosylated spike protein (S)	189:225	highly glycosylated spike protein (S)	189:225	HKU1 is a human beta coronavirus and infects host cells via highly glycosylated spike protein (S).					
34485242	1	22	theme	spike	209:213	arg1	S					224:224	highly glycosylated spike protein (S)	189:225	highly glycosylated spike protein (S)	189:225	HKU1 is a human beta coronavirus and infects host cells via highly glycosylated spike protein (S).					
34485242	7	23	link	O-linked	842:849	arg1	glycans					851:857	the O-linked glycans	838:857	the O-linked glycans	838:857	Moreover, the O-linked glycans and their abundance on each HKU1 S1 site were analyzed.					
34485242	6	24	theme	method	677:682	arg1	application					648:658	The application	644:658	The application of the developed method to the HKU1 S1 subunit	644:705	The application of the developed method to the HKU1 S1 subunit resulted in 46 novel O-glycosylation sites, among which 55.6% were predicted to be exposed on the outer protein surface.					
34485242	1	25	theme	protein	215:221	arg1	S					224:224	highly glycosylated spike protein (S)	189:225	highly glycosylated spike protein (S)	189:225	HKU1 is a human beta coronavirus and infects host cells via highly glycosylated spike protein (S).					
34485242	0	26	theme	Spike	40:44	arg1	Protein					46:52	HKU1 Spike Protein	35:52	HKU1 Spike Protein	35:52	Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials.					
34485242	4	27	theme	O-glycosylation	432:446	arg1	study					423:427	a comprehensive study	407:427	a comprehensive study of O-glycosylation of HKU1 S	407:456	Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials.					
34485242	6	28	theme	developed	667:675	arg1	method					677:682	the developed method	663:682	the developed method	663:682	The application of the developed method to the HKU1 S1 subunit resulted in 46 novel O-glycosylation sites, among which 55.6% were predicted to be exposed on the outer protein surface.					
34485242	0	29	theme	HKU1	35:38	arg1	Protein					46:52	HKU1 Spike Protein	35:52	HKU1 Spike Protein	35:52	Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials.					
34485242	2	30	gly	N-glycosylation	232:246	arg1	S					256:256	HKU1 S	251:256	HKU1 S	251:256	The N-glycosylation of HKU1 S has been reported.					
34485242	4	31	theme	S	456:456	arg1	O-glycosylation					432:446	O-glycosylation	432:446	O-glycosylation of HKU1 S	432:456	Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials.					
34485242	3	32	theme	functions	388:396	arg1	understanding					356:368	the in-depth understanding	343:368	the in-depth understanding of its biological functions	343:396	However, little is known about its O-glycosylation, which hinders the in-depth understanding of its biological functions.					
34485242	4	33	theme	HKU1	451:454	arg1	S					456:456	HKU1 S	451:456	HKU1 S	451:456	Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials.					
34485242	0	34	gly	O-Glycosylation	16:30	arg1	Protein					46:52	HKU1 Spike Protein	35:52	HKU1 Spike Protein	35:52	Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials.					
34485242	0	35	with	O-Glycosylation	16:30	arg1	Materials					118:126	the Dual-Functional Hydrophilic Interaction Chromatography Materials	59:126	the Dual-Functional Hydrophilic Interaction Chromatography Materials	59:126	Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials.					
34485242	0	36	theme	Protein	46:52	arg1	O-Glycosylation					16:30	the O-Glycosylation	12:30	the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials	12:126	Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials.					
34485242	4	37	theme	comprehensive	409:421	arg1	study					423:427	a comprehensive study	407:427	a comprehensive study of O-glycosylation of HKU1 S	407:456	Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials.					
34485242	5	38	theme	enrichment	544:553	arg1	method					555:560	The enrichment method	540:560	The enrichment method for O-glycopeptides with HBS	540:589	The enrichment method for O-glycopeptides with HBS was developed and validated using standard proteins.					
34485242	4	39	gly	O-glycosylation	432:446	arg1	S					456:456	HKU1 S	451:456	HKU1 S	451:456	Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials.					
34485242	6	40	theme	protein	811:817	arg1	surface					819:825	the outer protein surface	801:825	the outer protein surface	801:825	The application of the developed method to the HKU1 S1 subunit resulted in 46 novel O-glycosylation sites, among which 55.6% were predicted to be exposed on the outer protein surface.					
34485242	1	41	theme	human	139:143	arg1	coronavirus					150:160	a human beta coronavirus	137:160	a human beta coronavirus	137:160	HKU1 is a human beta coronavirus and infects host cells via highly glycosylated spike protein (S).					
34485242	1	41	theme	human	139:143	arg1	HKU1					129:132	HKU1	129:132	HKU1	129:132	HKU1 is a human beta coronavirus and infects host cells via highly glycosylated spike protein (S).					
34485242	7	42	theme	HKU1	887:890	arg1	site					895:898	each HKU1 S1 site	882:898	each HKU1 S1 site	882:898	Moreover, the O-linked glycans and their abundance on each HKU1 S1 site were analyzed.					
34485242	6	43	theme	outer	805:809	arg1	surface					819:825	the outer protein surface	801:825	the outer protein surface	801:825	The application of the developed method to the HKU1 S1 subunit resulted in 46 novel O-glycosylation sites, among which 55.6% were predicted to be exposed on the outer protein surface.					
34485242	2	44	theme	S	256:256	arg1	N-glycosylation					232:246	The N-glycosylation	228:246	The N-glycosylation of HKU1 S	228:256	The N-glycosylation of HKU1 S has been reported.					
34485242	6	45	theme	O-glycosylation	728:742	arg1	sites					744:748	46 novel O-glycosylation sites	719:748	46 novel O-glycosylation sites	719:748	The application of the developed method to the HKU1 S1 subunit resulted in 46 novel O-glycosylation sites, among which 55.6% were predicted to be exposed on the outer protein surface.					
34485242	1	46	theme	beta	145:148	arg1	coronavirus					150:160	a human beta coronavirus	137:160	a human beta coronavirus	137:160	HKU1 is a human beta coronavirus and infects host cells via highly glycosylated spike protein (S).					
34485242	1	46	theme	beta	145:148	arg1	HKU1					129:132	HKU1	129:132	HKU1	129:132	HKU1 is a human beta coronavirus and infects host cells via highly glycosylated spike protein (S).					
34485242	6	47	gly	O-glycosylation	728:742	arg2	sites					744:748	46 novel O-glycosylation sites	719:748	46 novel O-glycosylation sites	719:748	The application of the developed method to the HKU1 S1 subunit resulted in 46 novel O-glycosylation sites, among which 55.6% were predicted to be exposed on the outer protein surface.					
34485242	6	47	gly	O-glycosylation	728:742	arg2	46					719:720	46	719:720	46	719:720	The application of the developed method to the HKU1 S1 subunit resulted in 46 novel O-glycosylation sites, among which 55.6% were predicted to be exposed on the outer protein surface.					
34485242	0	48	theme	Dual-Functional	63:77	arg1	Materials					118:126	the Dual-Functional Hydrophilic Interaction Chromatography Materials	59:126	the Dual-Functional Hydrophilic Interaction Chromatography Materials	59:126	Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials.					
34485242	2	49	theme	HKU1	251:254	arg1	S					256:256	HKU1 S	251:256	HKU1 S	251:256	The N-glycosylation of HKU1 S has been reported.					
34485242	6	50	theme	novel	722:726	arg1	sites					744:748	46 novel O-glycosylation sites	719:748	46 novel O-glycosylation sites	719:748	The application of the developed method to the HKU1 S1 subunit resulted in 46 novel O-glycosylation sites, among which 55.6% were predicted to be exposed on the outer protein surface.					
34485242	4	51	theme	dual-functional	483:497	arg1	HBS					524:526	HBS	524:526	HBS	524:526	Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials.					
34485242	4	51	theme	dual-functional	483:497	arg1	silica					516:521	dual-functional histidine-bonded silica	483:521	dual-functional histidine-bonded silica (HBS) materials	483:537	Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica (HBS) materials.					
34485242	8	52	theme	O-glycosylation	928:942	arg1	dataset					944:950	The obtained O-glycosylation dataset	915:950	The obtained O-glycosylation dataset	915:950	The obtained O-glycosylation dataset will provide valuable insights into the structure of HKU1 S.					
34485242	0	53	theme	Chromatography	103:116	arg1	Materials					118:126	the Dual-Functional Hydrophilic Interaction Chromatography Materials	59:126	the Dual-Functional Hydrophilic Interaction Chromatography Materials	59:126	Deciphering the O-Glycosylation of HKU1 Spike Protein With the Dual-Functional Hydrophilic Interaction Chromatography Materials.					
32918890	6	0	theme	aggrecan	965:972	arg1	addition					953:960	addition	953:960	addition of aggrecan	953:972	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	8	1	theme	glycosylation	1464:1476	arg1	degrees					1453:1459	varying degrees	1445:1459	varying degrees of glycosylation	1445:1476	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	3	2	theme	HA-aggrecan	544:554	arg1	brush					556:560	the HA-aggrecan brush	540:560	the HA-aggrecan brush	540:560	Studies using electron microscopy have imaged the HA-aggrecan brush but require adsorption to a surface, dramatically altering the complex from its native conformation.					
32918890	1	3	theme	joint	282:286	arg1	function					288:295	healthy joint function	274:295	healthy joint function	274:295	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan contribute to cartilage compression resistance and are necessary for healthy joint function.					
32918890	2	4	theme	quantitative	417:428	arg1	description					430:440	a quantitative description	415:440	a quantitative description that links their structure and mechanical response	415:491	A variety of mechanical forces act on these complexes in the cartilage extracellular matrix, motivating the need for a quantitative description that links their structure and mechanical response.					
32918890	10	5	theme	longer	1804:1809	arg1	scales					1818:1823	much longer length scales	1799:1823	much longer length scales	1799:1823	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	10	6	from	distribution	1719:1730	arg1	HA					1756:1757	HA	1756:1757	HA	1756:1757	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	4	7	theme	mechanical	743:752	arg1	response					754:761	mechanical response	743:761	mechanical response	743:761	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	6	8	dep	forces	1088:1093	arg1	i.e.					1071:1074	i.e.	1071:1074	i.e.	1071:1074	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	11	9	theme	mechanical	1950:1959	arg1	properties					1961:1970	mechanical properties	1950:1970	mechanical properties	1950:1970	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	2	10	theme	forces	322:327	arg1	variety					300:306	A variety	298:306	A variety of mechanical forces	298:327	A variety of mechanical forces act on these complexes in the cartilage extracellular matrix, motivating the need for a quantitative description that links their structure and mechanical response.					
32918890	2	10	theme	forces	322:327	arg1	forces					322:327	mechanical forces	311:327	mechanical forces	311:327	A variety of mechanical forces act on these complexes in the cartilage extracellular matrix, motivating the need for a quantitative description that links their structure and mechanical response.					
32918890	10	11	theme	variable	1680:1687	arg1	glycosylation					1689:1701	variable glycosylation	1680:1701	variable glycosylation	1680:1701	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	6	12	theme	HA	1026:1027	arg1	chain					1029:1033	the HA chain	1022:1033	the HA chain	1022:1033	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	4	13	theme	aggrecan	781:788	arg1	monomers					790:797	aggrecan monomers	781:797	aggrecan monomers bind and form a bottlebrush	781:825	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	4	13	theme	aggrecan	781:788	arg1	changes					718:724	changes	718:724	changes in extension and mechanical response of an HA chain	718:776	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	4	14	theme	magnetic	670:677	arg1	tweezers					679:686	magnetic tweezers	670:686	magnetic tweezers	670:686	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	2	15	theme	mechanical	311:320	arg1	forces					322:327	mechanical forces	311:327	mechanical forces	311:327	A variety of mechanical forces act on these complexes in the cartilage extracellular matrix, motivating the need for a quantitative description that links their structure and mechanical response.					
32918890	10	16	theme	stochastic	1642:1651	arg1	model					1661:1665	a simple stochastic binding model	1633:1665	a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales	1633:1823	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	0	17	theme	Single-Molecule	0:14	arg1	Stretching					16:25	Single-Molecule Stretching	0:25	Single-Molecule Stretching	0:25	Single-Molecule Stretching Shows Glycosylation Sets Tension in the Hyaluronan-Aggrecan Bottlebrush.					
32918890	3	18	from	conformation	649:660	arg1	complex					625:631	the complex	621:631	the complex from its native conformation	621:660	Studies using electron microscopy have imaged the HA-aggrecan brush but require adsorption to a surface, dramatically altering the complex from its native conformation.					
32918890	10	19	theme	wide	1714:1717	arg1	distribution					1719:1730	a wide distribution	1712:1730	a wide distribution of internal tensions in HA	1712:1757	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	11	20	dep	architecture	1933:1944	arg1	the					1923:1925	the	1923:1925	the	1923:1925	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	8	21	theme	behavior	1298:1305	arg1	theories					1266:1273	theories	1266:1273	theories of bottlebrush polymer behavior	1266:1305	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	5	22	theme	single	884:889	arg1	complex					891:897	a single complex	882:897	a single complex with time and under varying solution conditions	882:945	This technique directly measures changes undergone by a single complex with time and under varying solution conditions.					
32918890	3	23	theme	electron	508:515	arg1	microscopy					517:526	electron microscopy	508:526	electron microscopy	508:526	Studies using electron microscopy have imaged the HA-aggrecan brush but require adsorption to a surface, dramatically altering the complex from its native conformation.					
32918890	8	24	theme	tension	1364:1370	arg1	consistent					1376:1385	consistent	1376:1385	consistent	1376:1385	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	8	24	theme	tension	1364:1370	arg1	values					1345:1350	the experimental values	1328:1350	the experimental values of internal tension	1328:1370	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	4	25	from	changes	718:724	arg1	extension					729:737	extension	729:737	extension	729:737	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	4	25	from	changes	718:724	arg1	response					754:761	mechanical response	743:761	mechanical response	743:761	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	1	26	theme	proteoglycan	183:194	arg1	aggrecan					196:203	the proteoglycan aggrecan	179:203	the proteoglycan aggrecan	179:203	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan contribute to cartilage compression resistance and are necessary for healthy joint function.					
32918890	4	27	dep	monomers	790:797	arg1	form					808:811	form	808:811	form a bottlebrush	808:825	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	4	27	dep	monomers	790:797	arg1	bind					799:802	bind	799:802	bind	799:802	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	0	28	from	Tension	52:58	arg1	Bottlebrush					87:97	the Hyaluronan-Aggrecan Bottlebrush	63:97	the Hyaluronan-Aggrecan Bottlebrush	63:97	Single-Molecule Stretching Shows Glycosylation Sets Tension in the Hyaluronan-Aggrecan Bottlebrush.					
32918890	1	29	theme	Large	100:104	arg1	complexes					118:126	Large bottlebrush complexes	100:126	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan	100:203	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan contribute to cartilage compression resistance and are necessary for healthy joint function.					
32918890	6	30	theme	swelling	991:998	arg1	effect					1000:1005	a large swelling effect	983:1005	a large swelling effect	983:1005	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	1	31	theme	bottlebrush	106:116	arg1	complexes					118:126	Large bottlebrush complexes	100:126	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan	100:203	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan contribute to cartilage compression resistance and are necessary for healthy joint function.					
32918890	2	32	theme	mechanical	473:482	arg1	response					484:491	mechanical response	473:491	mechanical response	473:491	A variety of mechanical forces act on these complexes in the cartilage extracellular matrix, motivating the need for a quantitative description that links their structure and mechanical response.					
32918890	9	33	theme	HA	1600:1601	arg1	stiffening					1603:1612	HA stiffening	1600:1612	HA stiffening	1600:1612	By enzymatically deglycosylating the aggrecan, we show that aggrecan glycosylation is the structural feature that causes HA stiffening.					
32918890	5	34	theme	varying	919:925	arg1	conditions					936:945	varying solution conditions	919:945	varying solution conditions	919:945	This technique directly measures changes undergone by a single complex with time and under varying solution conditions.					
32918890	0	35	theme	Glycosylation	33:45	arg1	Sets					47:50	Glycosylation Sets	33:50	Glycosylation Sets Tension in the Hyaluronan-Aggrecan Bottlebrush	33:97	Single-Molecule Stretching Shows Glycosylation Sets Tension in the Hyaluronan-Aggrecan Bottlebrush.					
32918890	11	36	theme	important	1980:1988	arg1	component					1990:1998	this important component	1975:1998	this important component of cartilage	1975:2011	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	5	37	with	complex	891:897	arg1	time					904:907	time	904:907	time	904:907	This technique directly measures changes undergone by a single complex with time and under varying solution conditions.					
32918890	1	38	theme	cartilage	219:227	arg1	resistance					241:250	cartilage compression resistance	219:250	cartilage compression resistance	219:250	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan contribute to cartilage compression resistance and are necessary for healthy joint function.					
32918890	6	39	dep	find	978:981	arg1	manifests					1007:1015	manifests	1007:1015	find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN)	978:1110	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	10	40	theme	tensions	1744:1751	arg1	distribution					1719:1730	a wide distribution	1712:1730	a wide distribution of internal tensions in HA	1712:1757	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	1	41	theme	compression	229:239	arg1	resistance					241:250	cartilage compression resistance	219:250	cartilage compression resistance	219:250	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan contribute to cartilage compression resistance and are necessary for healthy joint function.					
32918890	2	42	theme	cartilage	359:367	arg1	matrix					383:388	the cartilage extracellular matrix	355:388	the cartilage extracellular matrix	355:388	A variety of mechanical forces act on these complexes in the cartilage extracellular matrix, motivating the need for a quantitative description that links their structure and mechanical response.					
32918890	8	43	theme	aggrecan	1407:1414	arg1	population					1416:1425	a polydisperse aggrecan population	1392:1425	a polydisperse aggrecan population	1392:1425	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	6	44	theme	stretching	1077:1086	arg1	forces					1088:1093	stretching forces	1077:1093	stretching forces	1077:1093	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	8	45	theme	bottlebrush	1278:1288	arg1	behavior					1298:1305	bottlebrush polymer behavior	1278:1305	bottlebrush polymer behavior	1278:1305	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	0	46	theme	Sets	47:50	arg1	Tension					52:58	Glycosylation Sets Tension	33:58	Glycosylation Sets Tension in the Hyaluronan-Aggrecan Bottlebrush	33:97	Single-Molecule Stretching Shows Glycosylation Sets Tension in the Hyaluronan-Aggrecan Bottlebrush.					
32918890	6	47	theme	large	985:989	arg1	effect					1000:1005	a large swelling effect	983:1005	a large swelling effect	983:1005	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	10	48	theme	binding	1653:1659	arg1	model					1661:1665	a simple stochastic binding model	1633:1665	a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales	1633:1823	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	3	49	theme	native	642:647	arg1	conformation					649:660	its native conformation	638:660	its native conformation	638:660	Studies using electron microscopy have imaged the HA-aggrecan brush but require adsorption to a surface, dramatically altering the complex from its native conformation.					
32918890	8	50	with	consistent	1376:1385	arg1	population					1416:1425	a polydisperse aggrecan population	1392:1425	a polydisperse aggrecan population	1392:1425	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	4	51	theme	force	688:692	arg1	spectroscopy					694:705	force spectroscopy	688:705	force spectroscopy	688:705	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	7	52	theme	force-extension	1130:1144	arg1	behavior					1146:1153	force-extension behavior	1130:1153	force-extension behavior	1130:1153	We use models of force-extension behavior to show that repulsion between the aggrecans induces an internal tension in the HA chain.					
32918890	11	53	theme	mechanistic	1848:1858	arg1	picture					1860:1866	a mechanistic picture	1846:1866	a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage	1846:2011	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	10	54	from	scales	1818:1823	arg1	variations					1768:1777	variations	1768:1777	variations in the mechanics at much longer length scales	1768:1823	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	10	54	from	scales	1818:1823	arg1	mechanics					1786:1794	the mechanics	1782:1794	the mechanics at much longer length scales	1782:1823	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	7	55	theme	HA	1235:1236	arg1	chain					1238:1242	the HA chain	1231:1242	the HA chain	1231:1242	We use models of force-extension behavior to show that repulsion between the aggrecans induces an internal tension in the HA chain.					
32918890	10	56	theme	internal	1735:1742	arg1	tensions					1744:1751	internal tensions	1735:1751	internal tensions	1735:1751	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	2	57	theme	extracellular	369:381	arg1	matrix					383:388	the cartilage extracellular matrix	355:388	the cartilage extracellular matrix	355:388	A variety of mechanical forces act on these complexes in the cartilage extracellular matrix, motivating the need for a quantitative description that links their structure and mechanical response.					
32918890	7	58	theme	behavior	1146:1153	arg1	models					1120:1125	models	1120:1125	models of force-extension behavior	1120:1153	We use models of force-extension behavior to show that repulsion between the aggrecans induces an internal tension in the HA chain.					
32918890	11	59	theme	HA	1899:1900	arg1	size					1891:1894	size	1891:1894	size	1891:1894	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	11	59	theme	HA	1899:1900	arg1	flexibility					1875:1885	flexibility	1875:1885	flexibility	1875:1885	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	11	59	theme	HA	1899:1900	arg1	aggrecan					1906:1913	aggrecan	1906:1913	aggrecan	1906:1913	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	7	60	from	tension	1220:1226	arg1	chain					1238:1242	the HA chain	1231:1242	the HA chain	1231:1242	We use models of force-extension behavior to show that repulsion between the aggrecans induces an internal tension in the HA chain.					
32918890	10	61	theme	length	1811:1816	arg1	scales					1818:1823	much longer length scales	1799:1823	much longer length scales	1799:1823	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	7	62	theme	internal	1211:1218	arg1	tension					1220:1226	an internal tension	1208:1226	an internal tension in the HA chain	1208:1242	We use models of force-extension behavior to show that repulsion between the aggrecans induces an internal tension in the HA chain.					
32918890	1	63	theme	polysaccharide	144:157	arg1	hyaluronan					159:168	the polysaccharide hyaluronan	140:168	the polysaccharide hyaluronan (HA)	140:173	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan contribute to cartilage compression resistance and are necessary for healthy joint function.					
32918890	1	63	theme	polysaccharide	144:157	arg1	HA					171:172	HA	171:172	HA	171:172	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan contribute to cartilage compression resistance and are necessary for healthy joint function.					
32918890	10	64	from	variations	1768:1777	arg1	scales					1818:1823	much longer length scales	1799:1823	much longer length scales	1799:1823	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	10	64	from	variations	1768:1777	arg1	mechanics					1786:1794	the mechanics	1782:1794	the mechanics at much longer length scales	1782:1823	We then construct a simple stochastic binding model to show that variable glycosylation leads to a wide distribution of internal tensions in HA, causing variations in the mechanics at much longer length scales.					
32918890	8	65	theme	polydisperse	1394:1405	arg1	population					1416:1425	a polydisperse aggrecan population	1392:1425	a polydisperse aggrecan population	1392:1425	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	4	66	theme	chain	772:776	arg1	extension					729:737	extension	729:737	extension	729:737	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	4	66	theme	chain	772:776	arg1	response					754:761	mechanical response	743:761	mechanical response	743:761	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	6	67	theme	external	1053:1060	arg1	tension					1062:1068	external tension	1053:1068	very low external tension (i.e., stretching forces less than ∼1 pN)	1044:1110	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	6	67	theme	external	1053:1060	arg1	pN					1108:1109	less than ∼1 pN	1095:1109	less than ∼1 pN	1095:1109	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	8	68	theme	internal	1355:1362	arg1	tension					1364:1370	internal tension	1355:1370	internal tension	1355:1370	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	11	69	theme	cartilage	2003:2011	arg1	component					1990:1998	this important component	1975:1998	this important component of cartilage	1975:2011	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	4	70	theme	HA	769:770	arg1	chain					772:776	an HA chain	766:776	an HA chain	766:776	We use magnetic tweezers force spectroscopy to measure changes in extension and mechanical response of an HA chain as aggrecan monomers bind and form a bottlebrush.					
32918890	8	71	theme	experimental	1332:1343	arg1	consistent					1376:1385	consistent	1376:1385	consistent	1376:1385	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	8	71	theme	experimental	1332:1343	arg1	values					1345:1350	the experimental values	1328:1350	the experimental values of internal tension	1328:1370	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	6	72	theme	low	1049:1051	arg1	tension					1062:1068	external tension	1053:1068	very low external tension (i.e., stretching forces less than ∼1 pN)	1044:1110	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	6	72	theme	low	1049:1051	arg1	pN					1108:1109	less than ∼1 pN	1095:1109	less than ∼1 pN	1095:1109	Upon addition of aggrecan, we find a large swelling effect manifests when the HA chain is under very low external tension (i.e., stretching forces less than ∼1 pN).					
32918890	11	73	theme	brush	1927:1931	arg1	architecture					1933:1944	brush architecture	1927:1944	brush architecture	1927:1944	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	8	74	theme	varying	1445:1451	arg1	degrees					1453:1459	varying degrees	1445:1459	varying degrees of glycosylation	1445:1476	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	5	75	theme	solution	927:934	arg1	conditions					936:945	varying solution conditions	919:945	varying solution conditions	919:945	This technique directly measures changes undergone by a single complex with time and under varying solution conditions.					
32918890	9	76	theme	structural	1569:1578	arg1	feature					1580:1586	the structural feature	1565:1586	the structural feature that causes HA stiffening	1565:1612	By enzymatically deglycosylating the aggrecan, we show that aggrecan glycosylation is the structural feature that causes HA stiffening.					
32918890	9	76	theme	structural	1569:1578	arg1	glycosylation					1548:1560	aggrecan glycosylation	1539:1560	aggrecan glycosylation	1539:1560	By enzymatically deglycosylating the aggrecan, we show that aggrecan glycosylation is the structural feature that causes HA stiffening.					
32918890	0	77	theme	Hyaluronan-Aggrecan	67:85	arg1	Bottlebrush					87:97	the Hyaluronan-Aggrecan Bottlebrush	63:97	the Hyaluronan-Aggrecan Bottlebrush	63:97	Single-Molecule Stretching Shows Glycosylation Sets Tension in the Hyaluronan-Aggrecan Bottlebrush.					
32918890	11	78	theme	component	1990:1998	arg1	architecture					1933:1944	brush architecture	1927:1944	brush architecture	1927:1944	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	11	78	theme	component	1990:1998	arg1	properties					1961:1970	mechanical properties	1950:1970	mechanical properties	1950:1970	Our results provide a mechanistic picture of how flexibility and size of HA and aggrecan lead to the brush architecture and mechanical properties of this important component of cartilage.					
32918890	9	79	theme	aggrecan	1539:1546	arg1	feature					1580:1586	the structural feature	1565:1586	the structural feature that causes HA stiffening	1565:1612	By enzymatically deglycosylating the aggrecan, we show that aggrecan glycosylation is the structural feature that causes HA stiffening.					
32918890	9	79	theme	aggrecan	1539:1546	arg1	glycosylation					1548:1560	aggrecan glycosylation	1539:1560	aggrecan glycosylation	1539:1560	By enzymatically deglycosylating the aggrecan, we show that aggrecan glycosylation is the structural feature that causes HA stiffening.					
32918890	8	80	theme	polymer	1290:1296	arg1	behavior					1298:1305	bottlebrush polymer behavior	1278:1305	bottlebrush polymer behavior	1278:1305	Through reference to theories of bottlebrush polymer behavior, we demonstrate that the experimental values of internal tension are consistent with a polydisperse aggrecan population, likely caused by varying degrees of glycosylation.					
32918890	1	81	theme	healthy	274:280	arg1	function					288:295	healthy joint function	274:295	healthy joint function	274:295	Large bottlebrush complexes formed from the polysaccharide hyaluronan (HA) and the proteoglycan aggrecan contribute to cartilage compression resistance and are necessary for healthy joint function.					
33750987	2	0	link	N-linked	317:324	arg1	sites					340:344	N-linked glycosylation sites	317:344	N-linked glycosylation sites	317:344	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	2	0	link	N-linked	317:324	arg1	target					191:196	the principal target	177:196	the principal target of the immune response	177:219	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	5	1	theme	influenza	976:984	arg1	hemagglutinin					986:998	influenza hemagglutinin	976:998	influenza hemagglutinin	976:998	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	1	2	theme	human	156:160	arg1	population					162:171	the human population	152:171	the human population	152:171	Incessant antigenic evolution enables the persistence and spread of influenza virus in the human population.					
33750987	8	3	theme	glycan	1316:1321	arg1	evolution					1299:1307	the evolution	1295:1307	the evolution of the glycan shield on influenza virus	1295:1347	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	4	4	theme	H3N2	697:700	arg1	viruses					702:708	human seasonal influenza A H3N2 viruses	670:708	human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic	670:830	Here, we illustrate that 2G12 can also neutralize human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic.					
33750987	2	5	theme	principal	181:189	arg1	sites					340:344	N-linked glycosylation sites	317:344	N-linked glycosylation sites	317:344	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	2	5	theme	principal	181:189	arg1	target					191:196	the principal target	177:196	the principal target of the immune response	177:219	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	8	6	theme	2G12-like	1361:1369	arg1	neutralizers					1411:1422	broad neutralizers	1405:1422	broad neutralizers	1405:1422	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	8	6	theme	2G12-like	1361:1369	arg1	antibodies					1371:1380	2G12-like antibodies	1361:1380	2G12-like antibodies	1361:1380	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	8	7	from	virus	1343:1347	arg1	evolution					1299:1307	the evolution	1295:1307	the evolution of the glycan shield on influenza virus	1295:1347	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	4	8	theme	oligomannose-type	747:763	arg1	clusters					765:772	similar oligomannose-type clusters	739:772	similar oligomannose-type clusters	739:772	Here, we illustrate that 2G12 can also neutralize human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic.					
33750987	7	9	theme	strains	1257:1263	arg1	fitness					1234:1240	fitness	1234:1240	fitness of recent H3N2 strains	1234:1263	These two N-glycosylation sites have also become crucial for fitness of recent H3N2 strains.					
33750987	2	10	theme	influenza	264:272	arg1	viruses					274:280	influenza viruses	264:280	influenza viruses	264:280	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	2	11	theme	immunogenic	356:366	arg1	epitopes					376:383	immunogenic protein epitopes	356:383	immunogenic protein epitopes using host-derived glycans	356:410	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	1	12	theme	virus	143:147	arg1	spread					123:128	spread	123:128	spread	123:128	Incessant antigenic evolution enables the persistence and spread of influenza virus in the human population.					
33750987	1	12	theme	virus	143:147	arg1	persistence					107:117	persistence	107:117	persistence	107:117	Incessant antigenic evolution enables the persistence and spread of influenza virus in the human population.					
33750987	8	13	from	evolution	1299:1307	arg1	virus					1343:1347	influenza virus	1333:1347	influenza virus	1333:1347	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	5	14	theme	structural	839:848	arg1	biology					850:856	structural biology	839:856	structural biology	839:856	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	1	15	theme	antigenic	75:83	arg1	evolution					85:93	Incessant antigenic evolution	65:93	Incessant antigenic evolution	65:93	Incessant antigenic evolution enables the persistence and spread of influenza virus in the human population.					
33750987	3	16	dep	glycan	605:610	arg1	shield					612:617	shield	612:617	shield	612:617	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	5	17	theme	mass	862:865	arg1	spectrometric					867:879	mass spectrometric	862:879	mass spectrometric	862:879	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	7	18	theme	N-glycosylation	1183:1197	arg1	sites					1199:1203	These two N-glycosylation sites	1173:1203	These two N-glycosylation sites	1173:1203	These two N-glycosylation sites have also become crucial for fitness of recent H3N2 strains.					
33750987	6	19	theme	H3N2	1114:1117	arg1	strains					1119:1125	all human H3N2 strains	1104:1125	all human H3N2 strains	1104:1125	One of these glycan sites is highly conserved in all human H3N2 strains and the other emerged during virus evolution.					
33750987	8	20	from	glycan	1316:1321	arg1	virus					1343:1347	influenza virus	1333:1347	influenza virus	1333:1347	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	6	21	theme	virus	1156:1160	arg1	evolution					1162:1170	virus evolution	1156:1170	virus evolution	1156:1170	One of these glycan sites is highly conserved in all human H3N2 strains and the other emerged during virus evolution.					
33750987	5	22	theme	spectrometric	867:879	arg1	approaches					881:890	structural biology and mass spectrometric approaches	839:890	structural biology and mass spectrometric approaches	839:890	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	0	23	theme	cross-neutralizing	2:19	arg1	antibody					21:28	A cross-neutralizing antibody	0:28	A cross-neutralizing antibody between HIV-1 and influenza virus	0:62	A cross-neutralizing antibody between HIV-1 and influenza virus.					
33750987	7	24	gly	N-glycosylation	1183:1197	arg2	two					1179:1181	two	1179:1181	two	1179:1181	These two N-glycosylation sites have also become crucial for fitness of recent H3N2 strains.					
33750987	7	24	gly	N-glycosylation	1183:1197	arg2	sites					1199:1203	These two N-glycosylation sites	1173:1203	These two N-glycosylation sites	1173:1203	These two N-glycosylation sites have also become crucial for fitness of recent H3N2 strains.					
33750987	4	25	theme	similar	739:745	arg1	clusters					765:772	similar oligomannose-type clusters	739:772	similar oligomannose-type clusters	739:772	Here, we illustrate that 2G12 can also neutralize human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic.					
33750987	2	26	theme	host-derived	391:402	arg1	glycans					404:410	host-derived glycans	391:410	host-derived glycans	391:410	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	1	27	from	spread	123:128	arg1	population					162:171	the human population	152:171	the human population	152:171	Incessant antigenic evolution enables the persistence and spread of influenza virus in the human population.					
33750987	5	28	theme	receptor	945:952	arg1	RBS					968:970	RBS	968:970	RBS	968:970	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	5	28	theme	receptor	945:952	arg1	site					962:965	the receptor binding site	941:965	the receptor binding site (RBS) on influenza hemagglutinin	941:998	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	4	29	theme	human	670:674	arg1	viruses					702:708	human seasonal influenza A H3N2 viruses	670:708	human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic	670:830	Here, we illustrate that 2G12 can also neutralize human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic.					
33750987	6	30	theme	sites	1075:1079	arg1	One					1055:1057	One	1055:1057	One	1055:1057	One of these glycan sites is highly conserved in all human H3N2 strains and the other emerged during virus evolution.					
33750987	6	30	theme	sites	1075:1079	arg1	sites					1075:1079	these glycan sites	1062:1079	these glycan sites	1062:1079	One of these glycan sites is highly conserved in all human H3N2 strains and the other emerged during virus evolution.					
33750987	3	31	theme	dense	599:603	arg1	glycan					605:610	its dense glycan shield	595:617	its dense glycan shield	595:617	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	3	32	theme	under-processed	549:563	arg1	clusters					583:590	under-processed oligomannose-type clusters	549:590	under-processed oligomannose-type clusters on its dense glycan shield	549:617	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	5	33	theme	oligomannose	1014:1025	arg1	cluster					1027:1033	the oligomannose cluster	1010:1033	the oligomannose cluster recognized by 2G12	1010:1052	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	3	34	theme	envelope	468:475	arg1	Env					486:488	Env	486:488	Env	486:488	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	3	34	theme	envelope	468:475	arg1	protein					477:483	the HIV-1 envelope protein	458:483	the HIV-1 envelope protein (Env)	458:489	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	6	35	theme	human	1108:1112	arg1	strains					1119:1125	all human H3N2 strains	1104:1125	all human H3N2 strains	1104:1125	One of these glycan sites is highly conserved in all human H3N2 strains and the other emerged during virus evolution.					
33750987	8	36	dep	glycan	1316:1321	arg1	shield					1323:1328	shield	1323:1328	shield	1323:1328	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	5	37	gly	N-glycosylation	910:924	arg2	sites					926:930	two N-glycosylation sites	906:930	two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin	906:998	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	5	37	gly	N-glycosylation	910:924	arg2	two					906:908	two	906:908	two	906:908	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	2	38	link	host-derived	391:402	arg1	glycans					404:410	host-derived glycans	391:410	host-derived glycans	391:410	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	3	39	theme	oligomannose-type	565:581	arg1	clusters					583:590	under-processed oligomannose-type clusters	549:590	under-processed oligomannose-type clusters on its dense glycan shield	549:617	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	4	40	theme	A	695:695	arg1	viruses					702:708	human seasonal influenza A H3N2 viruses	670:708	human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic	670:830	Here, we illustrate that 2G12 can also neutralize human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic.					
33750987	8	41	theme	influenza	1333:1341	arg1	virus					1343:1347	influenza virus	1333:1347	influenza virus	1333:1347	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	4	42	theme	seasonal	676:683	arg1	viruses					702:708	human seasonal influenza A H3N2 viruses	670:708	human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic	670:830	Here, we illustrate that 2G12 can also neutralize human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic.					
33750987	1	43	theme	Incessant	65:73	arg1	evolution					85:93	Incessant antigenic evolution	65:93	Incessant antigenic evolution	65:93	Incessant antigenic evolution enables the persistence and spread of influenza virus in the human population.					
33750987	7	44	theme	recent	1245:1250	arg1	strains					1257:1263	recent H3N2 strains	1245:1263	recent H3N2 strains	1245:1263	These two N-glycosylation sites have also become crucial for fitness of recent H3N2 strains.					
33750987	2	45	theme	hemagglutinin	226:238	arg1	antigen					253:259	the hemagglutinin (HA) surface antigen	222:259	the hemagglutinin (HA) surface antigen on influenza viruses	222:280	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	5	46	theme	biology	850:856	arg1	approaches					881:890	structural biology and mass spectrometric approaches	839:890	structural biology and mass spectrometric approaches	839:890	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	6	47	theme	glycan	1068:1073	arg1	sites					1075:1079	these glycan sites	1062:1079	these glycan sites	1062:1079	One of these glycan sites is highly conserved in all human H3N2 strains and the other emerged during virus evolution.					
33750987	4	48	attach	present	731:737	arg1	their					777:781	their	777:781	their	777:781	Here, we illustrate that 2G12 can also neutralize human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic.					
33750987	4	48	attach	present	731:737	arg2	viruses					702:708	human seasonal influenza A H3N2 viruses	670:708	human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic	670:830	Here, we illustrate that 2G12 can also neutralize human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic.					
33750987	3	49	gly	glycosylated	523:534	arg1	Env					486:488	Env	486:488	Env	486:488	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	3	49	gly	glycosylated	523:534	arg1	protein					477:483	the HIV-1 envelope protein	458:483	the HIV-1 envelope protein (Env)	458:489	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	3	50	theme	HIV-1	462:466	arg1	Env					486:488	Env	486:488	Env	486:488	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	3	50	theme	HIV-1	462:466	arg1	protein					477:483	the HIV-1 envelope protein	458:483	the HIV-1 envelope protein (Env)	458:489	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	3	51	theme	Anti-glycan	413:423	arg1	antibodies					425:434	Anti-glycan antibodies	413:434	Anti-glycan antibodies	413:434	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	3	51	theme	Anti-glycan	413:423	arg1	2G12					445:448	2G12	445:448	2G12	445:448	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	5	52	theme	N-glycosylation	910:924	arg1	sites					926:930	two N-glycosylation sites	906:930	two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin	906:998	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	0	53	theme	influenza	48:56	arg1	virus					58:62	influenza virus	48:62	influenza virus	48:62	A cross-neutralizing antibody between HIV-1 and influenza virus.					
33750987	3	54	contain	contains	540:547	arg2	clusters					583:590	under-processed oligomannose-type clusters	549:590	under-processed oligomannose-type clusters on its dense glycan shield	549:617	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	3	54	contain	contains	540:547	arg1	Env					486:488	Env	486:488	Env	486:488	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	3	54	contain	contains	540:547	arg1	protein					477:483	the HIV-1 envelope protein	458:483	the HIV-1 envelope protein (Env)	458:489	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	2	55	theme	immune	205:210	arg1	response					212:219	the immune response	201:219	the immune response	201:219	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	2	56	theme	response	212:219	arg1	sites					340:344	N-linked glycosylation sites	317:344	N-linked glycosylation sites	317:344	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	2	56	theme	response	212:219	arg1	target					191:196	the principal target	177:196	the principal target of the immune response	177:219	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	1	57	from	persistence	107:117	arg1	population					162:171	the human population	152:171	the human population	152:171	Incessant antigenic evolution enables the persistence and spread of influenza virus in the human population.					
33750987	7	58	theme	H3N2	1252:1255	arg1	strains					1257:1263	recent H3N2 strains	1245:1263	recent H3N2 strains	1245:1263	These two N-glycosylation sites have also become crucial for fitness of recent H3N2 strains.					
33750987	5	59	theme	binding	954:960	arg1	RBS					968:970	RBS	968:970	RBS	968:970	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	5	59	theme	binding	954:960	arg1	site					962:965	the receptor binding site	941:965	the receptor binding site (RBS) on influenza hemagglutinin	941:998	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	2	60	theme	surface	245:251	arg1	antigen					253:259	the hemagglutinin (HA) surface antigen	222:259	the hemagglutinin (HA) surface antigen on influenza viruses	222:280	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	4	61	theme	influenza	685:693	arg1	viruses					702:708	human seasonal influenza A H3N2 viruses	670:708	human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic	670:830	Here, we illustrate that 2G12 can also neutralize human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic.					
33750987	1	62	theme	influenza	133:141	arg1	virus					143:147	influenza virus	133:147	influenza virus	133:147	Incessant antigenic evolution enables the persistence and spread of influenza virus in the human population.					
33750987	2	63	from	antigen	253:259	arg1	viruses					274:280	influenza viruses	264:280	influenza viruses	264:280	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	2	64	gly	glycosylation	326:338	arg2	target					191:196	the principal target	177:196	the principal target of the immune response	177:219	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	2	64	gly	glycosylation	326:338	arg2	sites					340:344	N-linked glycosylation sites	317:344	N-linked glycosylation sites	317:344	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	8	65	theme	enveloped	1440:1448	arg1	viruses					1450:1456	human enveloped viruses	1434:1456	human enveloped viruses	1434:1456	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	8	66	theme	broad	1405:1409	arg1	neutralizers					1411:1422	broad neutralizers	1405:1422	broad neutralizers	1405:1422	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	8	66	theme	broad	1405:1409	arg1	antibodies					1371:1380	2G12-like antibodies	1361:1380	2G12-like antibodies	1361:1380	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	2	67	theme	N-linked	317:324	arg1	sites					340:344	N-linked glycosylation sites	317:344	N-linked glycosylation sites	317:344	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	2	67	theme	N-linked	317:324	arg1	target					191:196	the principal target	177:196	the principal target of the immune response	177:219	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	3	68	from	clusters	583:590	arg1	glycan					605:610	its dense glycan shield	595:617	its dense glycan shield	595:617	Anti-glycan antibodies, such as 2G12, target the HIV-1 envelope protein (Env), which is even more extensively glycosylated and contains under-processed oligomannose-type clusters on its dense glycan shield.					
33750987	2	69	theme	glycosylation	326:338	arg1	sites					340:344	N-linked glycosylation sites	317:344	N-linked glycosylation sites	317:344	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	2	69	theme	glycosylation	326:338	arg1	target					191:196	the principal target	177:196	the principal target of the immune response	177:219	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33750987	8	70	theme	human	1434:1438	arg1	viruses					1450:1456	human enveloped viruses	1434:1456	human enveloped viruses	1434:1456	These findings shed light on the evolution of the glycan shield on influenza virus and suggest 2G12-like antibodies can potentially act as broad neutralizers to target human enveloped viruses.					
33750987	1	71	dep	persistence	107:117	arg1	the					103:105	the	103:105	the	103:105	Incessant antigenic evolution enables the persistence and spread of influenza virus in the human population.					
33750987	5	72	from	site	962:965	arg1	hemagglutinin					986:998	influenza hemagglutinin	976:998	influenza hemagglutinin	976:998	Using structural biology and mass spectrometric approaches, we find that two N-glycosylation sites close to the receptor binding site (RBS) on influenza hemagglutinin represent the oligomannose cluster recognized by 2G12.					
33750987	4	73	dep	their	777:781	arg1	HAs					783:785	HAs	783:785	HAs	783:785	Here, we illustrate that 2G12 can also neutralize human seasonal influenza A H3N2 viruses that have evolved to present similar oligomannose-type clusters on their HAs from around 20 years after the 1968 pandemic.					
33750987	2	74	theme	protein	368:374	arg1	epitopes					376:383	immunogenic protein epitopes	356:383	immunogenic protein epitopes using host-derived glycans	356:410	As the principal target of the immune response, the hemagglutinin (HA) surface antigen on influenza viruses continuously acquires and replaces N-linked glycosylation sites to shield immunogenic protein epitopes using host-derived glycans.					
33395290	0	0	theme	Glycosphingolipid	89:105	arg1	LcGg4					107:111	Glycosphingolipid LcGg4	89:111	Glycosphingolipid LcGg4	89:111	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.					
33395290	2	1	theme	universal	360:368	arg1	structures					318:327	the core structures	309:327	the core structures of GSL glycan and lipid	309:351	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	1	theme	universal	360:368	arg1	material					379:386	the universal starting material	356:386	the universal starting material	356:386	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	3	2	theme	cells	717:721	arg1	biomarker					669:677	a biomarker	667:677	a biomarker of undifferentiated malignant myeloid cells	667:721	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	2	theme	cells	717:721	arg1	LcGg4					651:655	LcGg4	651:655	LcGg4	651:655	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	3	contain	carrying	769:776	arg2	functionalities					812:826	unique functionalities	805:826	unique functionalities	805:826	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	3	contain	carrying	769:776	arg2	chains					794:799	different sugar chains	778:799	different sugar chains	778:799	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	3	contain	carrying	769:776	arg1	analogues					744:752	its analogues	740:752	its analogues	740:752	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	0	4	theme	LcGg4	107:111	arg1	Synthesis					76:84	Synthesis	76:84	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.	0:146	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.					
33395290	4	5	used	utilized	920:927	arg2	strategy					864:871	This synthetic strategy	849:871	This synthetic strategy	849:871	This synthetic strategy should be widely applicable and, therefore, be utilized to rapidly access various GSLs and related derivatives by using different donors for glycosylations and different substrates for lipid remodeling following each glycosylation.					
33395290	2	6	theme	chemoselective	533:546	arg1	cross-metathesis					548:563	chemoselective cross-metathesis	533:563	chemoselective cross-metathesis	533:563	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	3	7	theme	analogues	744:752	arg1	biomarker					669:677	a biomarker	667:677	a biomarker of undifferentiated malignant myeloid cells	667:721	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	7	theme	analogues	744:752	arg1	LcGg4					651:655	LcGg4	651:655	LcGg4	651:655	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	7	theme	analogues	744:752	arg1	series					730:735	a series	728:735	a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels	728:846	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	7	theme	analogues	744:752	arg1	GSL					646:648	a lacto-ganglio GSL	630:648	a lacto-ganglio GSL	630:648	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	2	8	theme	synthetic	408:416	arg1	targets					418:424	different synthetic targets	398:424	different synthetic targets	398:424	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	3	9	theme	series	730:735	arg1	synthesis					617:625	the synthesis	613:625	the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels	613:846	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	4	10	theme	different	1033:1041	arg1	substrates					1043:1052	different substrates	1033:1052	different substrates for lipid remodeling following each glycosylation	1033:1102	This synthetic strategy should be widely applicable and, therefore, be utilized to rapidly access various GSLs and related derivatives by using different donors for glycosylations and different substrates for lipid remodeling following each glycosylation.					
33395290	2	11	theme	simple	281:286	arg1	lactoside					288:296	a simple lactoside	279:296	a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material	279:386	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	12	theme	different	398:406	arg1	targets					418:424	different synthetic targets	398:424	different synthetic targets	398:424	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	0	13	theme	Diversity-Oriented	2:19	arg1	Strategy					21:28	A Diversity-Oriented Strategy	0:28	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.	0:146	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.					
33395290	2	14	theme	GSL	332:334	arg1	glycan					336:341	GSL glycan	332:341	GSL glycan	332:341	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	15	theme	glycan	458:463	arg1	elongation					440:449	stepwise elongation	431:449	stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation	431:579	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	4	16	theme	lipid	1058:1062	arg1	remodeling					1064:1073	lipid remodeling	1058:1073	lipid remodeling following each glycosylation	1058:1102	This synthetic strategy should be widely applicable and, therefore, be utilized to rapidly access various GSLs and related derivatives by using different donors for glycosylations and different substrates for lipid remodeling following each glycosylation.					
33395290	4	17	theme	each	1085:1088	arg1	glycosylation					1090:1102	each glycosylation	1085:1102	each glycosylation	1085:1102	This synthetic strategy should be widely applicable and, therefore, be utilized to rapidly access various GSLs and related derivatives by using different donors for glycosylations and different substrates for lipid remodeling following each glycosylation.					
33395290	3	18	theme	different	778:786	arg1	chains					794:799	different sugar chains	778:799	different sugar chains	778:799	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	4	19	theme	various	947:953	arg1	GSLs					955:958	various GSLs	947:958	various GSLs	947:958	This synthetic strategy should be widely applicable and, therefore, be utilized to rapidly access various GSLs and related derivatives by using different donors for glycosylations and different substrates for lipid remodeling following each glycosylation.					
33395290	4	20	theme	synthetic	854:862	arg1	strategy					864:871	This synthetic strategy	849:871	This synthetic strategy	849:871	This synthetic strategy should be widely applicable and, therefore, be utilized to rapidly access various GSLs and related derivatives by using different donors for glycosylations and different substrates for lipid remodeling following each glycosylation.					
33395290	1	21	theme	glycosphingolipids	213:230	arg1	synthesis					200:208	the synthesis	196:208	the synthesis of glycosphingolipids (GSLs)	196:237	A diversity-oriented strategy was developed for the synthesis of glycosphingolipids (GSLs).					
33395290	2	22	contain	containing	298:307	arg2	material					379:386	the universal starting material	356:386	the universal starting material	356:386	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	22	contain	containing	298:307	arg2	structures					318:327	the core structures	309:327	the core structures of GSL glycan and lipid	309:351	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	22	contain	containing	298:307	arg1	lactoside					288:296	a simple lactoside	279:296	a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material	279:386	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	23	theme	core	313:316	arg1	structures					318:327	the core structures	309:327	the core structures of GSL glycan and lipid	309:351	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	23	theme	core	313:316	arg1	material					379:386	the universal starting material	356:386	the universal starting material	356:386	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	24	theme	stepwise	431:438	arg1	elongation					440:449	stepwise elongation	431:449	stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation	431:579	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	25	theme	on-site	497:503	arg1	remodeling					505:514	on-site remodeling	497:514	on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation	497:579	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	3	26	theme	derivatives	757:767	arg1	biomarker					669:677	a biomarker	667:677	a biomarker of undifferentiated malignant myeloid cells	667:721	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	26	theme	derivatives	757:767	arg1	LcGg4					651:655	LcGg4	651:655	LcGg4	651:655	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	26	theme	derivatives	757:767	arg1	series					730:735	a series	728:735	a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels	728:846	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	26	theme	derivatives	757:767	arg1	GSL					646:648	a lacto-ganglio GSL	630:648	a lacto-ganglio GSL	630:648	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	0	27	theme	Analogues	121:129	arg1	Synthesis					76:84	Synthesis	76:84	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.	0:146	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.					
33395290	0	28	theme	Chemical	34:41	arg1	Synthesis					43:51	Chemical Synthesis	34:51	Chemical Synthesis of Glycosphingolipids	34:73	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.					
33395290	3	29	theme	lacto-ganglio	632:644	arg1	GSL					646:648	a lacto-ganglio GSL	630:648	a lacto-ganglio GSL	630:648	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	0	30	theme	Glycosphingolipids	56:73	arg1	Synthesis					43:51	Chemical Synthesis	34:51	Chemical Synthesis of Glycosphingolipids	34:73	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.					
33395290	3	31	theme	unique	805:810	arg1	functionalities					812:826	unique functionalities	805:826	unique functionalities	805:826	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	2	32	theme	chemical	469:476	arg1	glycosylations					478:491	chemical glycosylations	469:491	chemical glycosylations	469:491	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	3	33	theme	sugar	788:792	arg1	chains					794:799	different sugar chains	778:799	different sugar chains	778:799	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	34	theme	malignant	699:707	arg1	cells					717:721	undifferentiated malignant myeloid cells	682:721	undifferentiated malignant myeloid cells	682:721	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	2	35	gly	glycosylations	478:491	arg1	lipid					523:527	the lipid	519:527	the lipid via chemoselective cross-metathesis and N-acylation	519:579	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	36	theme	lipid	347:351	arg1	structures					318:327	the core structures	309:327	the core structures of GSL glycan and lipid	309:351	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	36	theme	lipid	347:351	arg1	material					379:386	the universal starting material	356:386	the universal starting material	356:386	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	3	37	theme	myeloid	709:715	arg1	cells					717:721	undifferentiated malignant myeloid cells	682:721	undifferentiated malignant myeloid cells	682:721	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	38	theme	LcGg4	651:655	arg1	synthesis					617:625	the synthesis	613:625	the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels	613:846	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	39	theme	molecular	831:839	arg1	labels					841:846	molecular labels	831:846	molecular labels	831:846	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	0	40	dep	Strategy	21:28	arg1	Synthesis					76:84	Synthesis	76:84	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.	0:146	A Diversity-Oriented Strategy for Chemical Synthesis of Glycosphingolipids: Synthesis of Glycosphingolipid LcGg4 and Its Analogues and Derivatives.					
33395290	2	41	theme	glycan	336:341	arg1	structures					318:327	the core structures	309:327	the core structures of GSL glycan and lipid	309:351	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	41	theme	glycan	336:341	arg1	material					379:386	the universal starting material	356:386	the universal starting material	356:386	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	42	theme	lipid	523:527	arg1	glycosylations					478:491	chemical glycosylations	469:491	chemical glycosylations	469:491	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	42	theme	lipid	523:527	arg1	remodeling					505:514	on-site remodeling	497:514	on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation	497:579	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	3	43	theme	undifferentiated	682:697	arg1	cells					717:721	undifferentiated malignant myeloid cells	682:721	undifferentiated malignant myeloid cells	682:721	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	3	44	theme	GSL	646:648	arg1	synthesis					617:625	the synthesis	613:625	the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels	613:846	The strategy was verified with the synthesis of a lacto-ganglio GSL, LcGg4, which is a biomarker of undifferentiated malignant myeloid cells, and a series of its analogues or derivatives carrying different sugar chains and unique functionalities or molecular labels.					
33395290	1	45	theme	diversity-oriented	150:167	arg1	strategy					169:176	A diversity-oriented strategy	148:176	A diversity-oriented strategy	148:176	A diversity-oriented strategy was developed for the synthesis of glycosphingolipids (GSLs).					
33395290	4	46	dep	applicable	890:899	arg1	utilized					920:927	utilized	920:927	be utilized to rapidly access various GSLs and related derivatives by using different donors for glycosylations and different substrates for lipid remodeling following each glycosylation	917:1102	This synthetic strategy should be widely applicable and, therefore, be utilized to rapidly access various GSLs and related derivatives by using different donors for glycosylations and different substrates for lipid remodeling following each glycosylation.					
33395290	4	47	theme	different	993:1001	arg1	donors					1003:1008	different donors	993:1008	different donors for glycosylations and different substrates for lipid remodeling following each glycosylation	993:1102	This synthetic strategy should be widely applicable and, therefore, be utilized to rapidly access various GSLs and related derivatives by using different donors for glycosylations and different substrates for lipid remodeling following each glycosylation.					
33395290	2	48	theme	starting	370:377	arg1	structures					318:327	the core structures	309:327	the core structures of GSL glycan and lipid	309:351	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	2	48	theme	starting	370:377	arg1	material					379:386	the universal starting material	356:386	the universal starting material	356:386	This strategy was highlighted by using a simple lactoside containing the core structures of GSL glycan and lipid as the universal starting material to obtain different synthetic targets upon stepwise elongation of the glycan via chemical glycosylations and on-site remodeling of the lipid via chemoselective cross-metathesis and N-acylation.					
33395290	4	49	theme	related	964:970	arg1	derivatives					972:982	related derivatives	964:982	related derivatives	964:982	This synthetic strategy should be widely applicable and, therefore, be utilized to rapidly access various GSLs and related derivatives by using different donors for glycosylations and different substrates for lipid remodeling following each glycosylation.					
33245474	13	0	from	alteration	1723:1732	arg1	presence					1741:1748	the presence	1737:1748	the presence of miglustat	1737:1761	Our work shows that despite clear N-glycan alteration in the presence of miglustat, the functions of the Covid-19-related glycoproteins studied were not affected, making it unlikely that miglustat can change the natural course of the disease.					
33245474	6	1	theme	SARS-CoV-2	941:950	arg1	S					952:952	SARS-CoV-2 S	941:952	SARS-CoV-2 S	941:952	Cell-to-cell fusion was assessed by immunofluorescence of cocultures of SARS-CoV-2 S and ACE2-expressing cells.					
33245474	0	2	with	Changes	71:77	arg1	Effects					103:109	Limited Functional Effects	84:109	Limited Functional Effects	84:109	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.					
33245474	13	3	theme	clear	1708:1712	arg1	alteration					1723:1732	clear N-glycan alteration	1708:1732	clear N-glycan alteration in the presence of miglustat	1708:1761	Our work shows that despite clear N-glycan alteration in the presence of miglustat, the functions of the Covid-19-related glycoproteins studied were not affected, making it unlikely that miglustat can change the natural course of the disease.					
33245474	8	4	theme	receptor	1225:1232	arg1	ACE2					1234:1237	its main receptor ACE2	1216:1237	its main receptor ACE2	1216:1237	In our overexpression system, miglustat successfully and specifically modified N-glycans in both SARS-CoV-2 S and its main receptor ACE2.					
33245474	6	5	theme	cocultures	927:936	arg1	immunofluorescence					905:922	immunofluorescence	905:922	immunofluorescence of cocultures of SARS-CoV-2 S and ACE2-expressing cells	905:978	Cell-to-cell fusion was assessed by immunofluorescence of cocultures of SARS-CoV-2 S and ACE2-expressing cells.					
33245474	2	6	theme	glycan	264:269	arg1	alteration					271:280	The glycan alteration	260:280	The glycan alteration derived from this disease	260:306	The glycan alteration derived from this disease resulted in an in vitro infection resistance to particular enveloped, N-glycosylation-dependent viruses as influenza and HIV.					
33245474	1	7	theme	congenital	158:167	arg1	disorder					169:176	a rare congenital disorder	151:176	a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS)	151:257	In 2014, we reported two siblings with a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS).					
33245474	4	8	theme	human	686:690	arg1	GP					712:713	GP	712:713	GP	712:713	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	4	8	theme	human	686:690	arg1	glycoproteins					697:709	human ACE2 glycoproteins	686:709	human ACE2 glycoproteins (GP)	686:714	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	3	9	theme	FDA-approved	565:576	arg1	miglustat					602:610	the FDA-approved α-glucosidase inhibitor miglustat	561:610	the FDA-approved α-glucosidase inhibitor miglustat	561:610	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	13	10	dep	studied	1816:1822	arg1	affected					1833:1840	affected	1833:1840	affected	1833:1840	Our work shows that despite clear N-glycan alteration in the presence of miglustat, the functions of the Covid-19-related glycoproteins studied were not affected, making it unlikely that miglustat can change the natural course of the disease.					
33245474	4	11	theme	SARS-CoV-2	661:670	arg1	spike					672:676	SARS-CoV-2 spike	661:676	SARS-CoV-2 spike (S)	661:680	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	4	11	theme	SARS-CoV-2	661:670	arg1	S					679:679	S	679:679	S	679:679	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	7	12	from	PBMC	1063:1066	arg1	release					1050:1056	cytokine release	1041:1056	cytokine release from PBMC exposed to purified SARS-CoV-2 S	1041:1099	Miglustat effect on immune response was tested by measuring cytokine release from PBMC exposed to purified SARS-CoV-2 S.					
33245474	4	13	gly	glycoproteins	697:709	arg1	GP					712:713	GP	712:713	GP	712:713	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	4	13	gly	glycoproteins	697:709	arg1	glycoproteins					697:709	human ACE2 glycoproteins	686:709	human ACE2 glycoproteins (GP)	686:714	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	3	14	theme	inhibitor	592:600	arg1	miglustat					602:610	the FDA-approved α-glucosidase inhibitor miglustat	561:610	the FDA-approved α-glucosidase inhibitor miglustat	561:610	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	6	15	theme	cells	974:978	arg1	cocultures					927:936	cocultures	927:936	cocultures of SARS-CoV-2 S and ACE2-expressing cells	927:978	Cell-to-cell fusion was assessed by immunofluorescence of cocultures of SARS-CoV-2 S and ACE2-expressing cells.					
33245474	7	16	theme	purified	1079:1086	arg1	S					1099:1099	purified SARS-CoV-2 S	1079:1099	purified SARS-CoV-2 S	1079:1099	Miglustat effect on immune response was tested by measuring cytokine release from PBMC exposed to purified SARS-CoV-2 S.					
33245474	0	17	theme	Structural	60:69	arg1	Changes					71:77	In vitro Structural Changes	51:77	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.	0:110	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.					
33245474	12	18	gly	glycoprotein	1646:1657	arg1	glycoprotein					1646:1657	SARS-CoV-2 S glycoprotein stimulation	1633:1669	SARS-CoV-2 S glycoprotein stimulation of PBMC	1633:1677	Miglustat produced no statistically significant effects on cytokine production following SARS-CoV-2 S glycoprotein stimulation of PBMC.					
33245474	12	19	theme	PBMC	1674:1677	arg1	stimulation					1659:1669	SARS-CoV-2 S glycoprotein stimulation	1633:1669	SARS-CoV-2 S glycoprotein stimulation of PBMC	1633:1677	Miglustat produced no statistically significant effects on cytokine production following SARS-CoV-2 S glycoprotein stimulation of PBMC.					
33245474	13	20	gly	glycoproteins	1802:1814	arg1	glycoproteins					1802:1814	the Covid-19-related glycoproteins	1781:1814	the Covid-19-related glycoproteins	1781:1814	Our work shows that despite clear N-glycan alteration in the presence of miglustat, the functions of the Covid-19-related glycoproteins studied were not affected, making it unlikely that miglustat can change the natural course of the disease.					
33245474	4	21	theme	α-glucosidase	774:786	arg1	inhibition					788:797	α-glucosidase inhibition	774:797	α-glucosidase inhibition	774:797	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	0	22	theme	Functional	92:101	arg1	Effects					103:109	Limited Functional Effects	84:109	Limited Functional Effects	84:109	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.					
33245474	1	23	with	siblings	137:144	arg1	disorder					169:176	a rare congenital disorder	151:176	a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS)	151:257	In 2014, we reported two siblings with a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS).					
33245474	12	24	theme	S	1644:1644	arg1	stimulation					1659:1669	SARS-CoV-2 S glycoprotein stimulation	1633:1669	SARS-CoV-2 S glycoprotein stimulation of PBMC	1633:1677	Miglustat produced no statistically significant effects on cytokine production following SARS-CoV-2 S glycoprotein stimulation of PBMC.					
33245474	10	25	theme	viral	1334:1338	arg1	effect					1351:1356	viral cytopathic effect	1334:1356	viral cytopathic effect	1334:1356	A surrogate marker for viral cytopathic effect, measured as receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion, was not disrupted by miglustat treatment.					
33245474	7	26	theme	Miglustat	981:989	arg1	effect					991:996	Miglustat effect	981:996	Miglustat effect on immune response	981:1015	Miglustat effect on immune response was tested by measuring cytokine release from PBMC exposed to purified SARS-CoV-2 S.					
33245474	13	27	theme	Covid-19-related	1785:1800	arg1	glycoproteins					1802:1814	the Covid-19-related glycoproteins	1781:1814	the Covid-19-related glycoproteins	1781:1814	Our work shows that despite clear N-glycan alteration in the presence of miglustat, the functions of the Covid-19-related glycoproteins studied were not affected, making it unlikely that miglustat can change the natural course of the disease.					
33245474	4	28	theme	N-glycan	740:747	arg1	modifications					749:761	N-glycan modifications	740:761	N-glycan modifications induced by α-glucosidase inhibition	740:797	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	0	29	theme	N-Glycan	0:7	arg1	Modification					9:20	N-Glycan Modification	0:20	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.	0:110	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.					
33245474	13	30	theme	miglustat	1753:1761	arg1	presence					1741:1748	the presence	1737:1748	the presence of miglustat	1737:1761	Our work shows that despite clear N-glycan alteration in the presence of miglustat, the functions of the Covid-19-related glycoproteins studied were not affected, making it unlikely that miglustat can change the natural course of the disease.					
33245474	5	31	used	used	824:827	arg2	Immunoprecipitation					800:818	Immunoprecipitation	800:818	Immunoprecipitation	800:818	Immunoprecipitation was used to assess binding between these two GP.					
33245474	3	32	theme	effort	456:461	arg1	part					437:440	part	437:440	part of the global effort to find safe and effective antiviral therapies for Covid-19	437:521	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	0	33	dep	In	51:52	arg1	vitro					54:58	vitro	54:58	vitro	54:58	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.					
33245474	10	34	theme	S-driven	1401:1408	arg1	fusion					1423:1428	receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion	1371:1428	receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion	1371:1428	A surrogate marker for viral cytopathic effect, measured as receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion, was not disrupted by miglustat treatment.					
33245474	3	35	theme	antiviral	490:498	arg1	therapies					500:508	safe and effective antiviral therapies	471:508	safe and effective antiviral therapies for Covid-19	471:521	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	0	36	theme	In	51:52	arg1	Changes					71:77	In vitro Structural Changes	51:77	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.	0:110	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.					
33245474	6	37	theme	Cell-to-cell	869:880	arg1	fusion					882:887	Cell-to-cell fusion	869:887	Cell-to-cell fusion	869:887	Cell-to-cell fusion was assessed by immunofluorescence of cocultures of SARS-CoV-2 S and ACE2-expressing cells.					
33245474	10	38	theme	receptor-dependent	1371:1388	arg1	fusion					1423:1428	receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion	1371:1428	receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion	1371:1428	A surrogate marker for viral cytopathic effect, measured as receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion, was not disrupted by miglustat treatment.					
33245474	12	39	theme	significant	1580:1590	arg1	effects					1592:1598	no statistically significant effects	1563:1598	no statistically significant effects on cytokine production	1563:1621	Miglustat produced no statistically significant effects on cytokine production following SARS-CoV-2 S glycoprotein stimulation of PBMC.					
33245474	13	40	theme	disease	1914:1920	arg1	course					1900:1905	the natural course	1888:1905	the natural course of the disease	1888:1920	Our work shows that despite clear N-glycan alteration in the presence of miglustat, the functions of the Covid-19-related glycoproteins studied were not affected, making it unlikely that miglustat can change the natural course of the disease.					
33245474	1	41	theme	glucosidase	240:250	arg1	MOGS					253:256	mannosyl-oligosaccharide glucosidase (MOGS)	215:257	mannosyl-oligosaccharide glucosidase (MOGS)	215:257	In 2014, we reported two siblings with a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS).					
33245474	6	42	theme	ACE2-expressing	958:972	arg1	cells					974:978	ACE2-expressing cells	958:978	ACE2-expressing cells	958:978	Cell-to-cell fusion was assessed by immunofluorescence of cocultures of SARS-CoV-2 S and ACE2-expressing cells.					
33245474	4	43	theme	Expression	632:641	arg1	plasmids					643:650	Expression plasmids	632:650	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP)	632:714	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	2	44	theme	particular	356:365	arg1	viruses					404:410	particular enveloped, N-glycosylation-dependent viruses	356:410	particular enveloped, N-glycosylation-dependent viruses as influenza and HIV	356:431	The glycan alteration derived from this disease resulted in an in vitro infection resistance to particular enveloped, N-glycosylation-dependent viruses as influenza and HIV.					
33245474	8	45	theme	SARS-CoV-2	1199:1208	arg1	S					1210:1210	SARS-CoV-2 S	1199:1210	SARS-CoV-2 S	1199:1210	In our overexpression system, miglustat successfully and specifically modified N-glycans in both SARS-CoV-2 S and its main receptor ACE2.					
33245474	6	46	theme	S	952:952	arg1	cocultures					927:936	cocultures	927:936	cocultures of SARS-CoV-2 S and ACE2-expressing cells	927:978	Cell-to-cell fusion was assessed by immunofluorescence of cocultures of SARS-CoV-2 S and ACE2-expressing cells.					
33245474	13	47	theme	natural	1892:1898	arg1	course					1900:1905	the natural course	1888:1905	the natural course of the disease	1888:1920	Our work shows that despite clear N-glycan alteration in the presence of miglustat, the functions of the Covid-19-related glycoproteins studied were not affected, making it unlikely that miglustat can change the natural course of the disease.					
33245474	10	48	theme	miglustat	1452:1460	arg1	treatment					1462:1470	miglustat treatment	1452:1470	miglustat treatment	1452:1470	A surrogate marker for viral cytopathic effect, measured as receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion, was not disrupted by miglustat treatment.					
33245474	3	49	theme	in	540:541	arg1	activity					549:556	the in vitro activity	536:556	the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2	536:629	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	13	50	theme	N-glycan	1714:1721	arg1	alteration					1723:1732	clear N-glycan alteration	1708:1732	clear N-glycan alteration in the presence of miglustat	1708:1761	Our work shows that despite clear N-glycan alteration in the presence of miglustat, the functions of the Covid-19-related glycoproteins studied were not affected, making it unlikely that miglustat can change the natural course of the disease.					
33245474	1	51	theme	rare	153:156	arg1	disorder					169:176	a rare congenital disorder	151:176	a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS)	151:257	In 2014, we reported two siblings with a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS).					
33245474	8	52	theme	main	1220:1223	arg1	ACE2					1234:1237	its main receptor ACE2	1216:1237	its main receptor ACE2	1216:1237	In our overexpression system, miglustat successfully and specifically modified N-glycans in both SARS-CoV-2 S and its main receptor ACE2.					
33245474	12	53	theme	SARS-CoV-2	1633:1642	arg1	stimulation					1659:1669	SARS-CoV-2 S glycoprotein stimulation	1633:1669	SARS-CoV-2 S glycoprotein stimulation of PBMC	1633:1677	Miglustat produced no statistically significant effects on cytokine production following SARS-CoV-2 S glycoprotein stimulation of PBMC.					
33245474	1	54	theme	glycosylation	181:193	arg1	disorder					169:176	a rare congenital disorder	151:176	a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS)	151:257	In 2014, we reported two siblings with a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS).					
33245474	12	55	from	effects	1592:1598	arg1	production					1612:1621	cytokine production	1603:1621	cytokine production	1603:1621	Miglustat produced no statistically significant effects on cytokine production following SARS-CoV-2 S glycoprotein stimulation of PBMC.					
33245474	3	56	theme	miglustat	602:610	arg1	activity					549:556	the in vitro activity	536:556	the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2	536:629	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	2	57	attach	derived	282:288	arg1	disease					300:306	this disease	295:306	this disease	295:306	The glycan alteration derived from this disease resulted in an in vitro infection resistance to particular enveloped, N-glycosylation-dependent viruses as influenza and HIV.					
33245474	2	57	attach	derived	282:288	arg2	alteration					271:280	The glycan alteration	260:280	The glycan alteration derived from this disease	260:306	The glycan alteration derived from this disease resulted in an in vitro infection resistance to particular enveloped, N-glycosylation-dependent viruses as influenza and HIV.					
33245474	7	58	theme	SARS-CoV-2	1088:1097	arg1	S					1099:1099	purified SARS-CoV-2 S	1079:1099	purified SARS-CoV-2 S	1079:1099	Miglustat effect on immune response was tested by measuring cytokine release from PBMC exposed to purified SARS-CoV-2 S.					
33245474	9	59	theme	glycan	1289:1294	arg1	modifications					1296:1308	glycan modifications	1289:1308	glycan modifications	1289:1308	Binding between these two GP was not affected by glycan modifications.					
33245474	3	60	theme	α-glucosidase	578:590	arg1	miglustat					602:610	the FDA-approved α-glucosidase inhibitor miglustat	561:610	the FDA-approved α-glucosidase inhibitor miglustat	561:610	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	1	61	from	mutations	202:210	arg1	MOGS					253:256	mannosyl-oligosaccharide glucosidase (MOGS)	215:257	mannosyl-oligosaccharide glucosidase (MOGS)	215:257	In 2014, we reported two siblings with a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS).					
33245474	3	62	dep	in	540:541	arg1	vitro					543:547	vitro	543:547	vitro	543:547	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	7	63	from	effect	991:996	arg1	response					1008:1015	immune response	1001:1015	immune response	1001:1015	Miglustat effect on immune response was tested by measuring cytokine release from PBMC exposed to purified SARS-CoV-2 S.					
33245474	7	64	theme	cytokine	1041:1048	arg1	release					1050:1056	cytokine release	1041:1056	cytokine release from PBMC exposed to purified SARS-CoV-2 S	1041:1099	Miglustat effect on immune response was tested by measuring cytokine release from PBMC exposed to purified SARS-CoV-2 S.					
33245474	4	65	theme	ACE2	692:695	arg1	GP					712:713	GP	712:713	GP	712:713	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	4	65	theme	ACE2	692:695	arg1	glycoproteins					697:709	human ACE2 glycoproteins	686:709	human ACE2 glycoproteins (GP)	686:714	Expression plasmids encoding SARS-CoV-2 spike (S) and human ACE2 glycoproteins (GP) were tested to evaluate N-glycan modifications induced by α-glucosidase inhibition.					
33245474	10	66	theme	surrogate	1313:1321	arg1	marker					1323:1328	A surrogate marker	1311:1328	A surrogate marker	1311:1328	A surrogate marker for viral cytopathic effect, measured as receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion, was not disrupted by miglustat treatment.					
33245474	0	67	theme	Limited	84:90	arg1	Effects					103:109	Limited Functional Effects	84:109	Limited Functional Effects	84:109	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.					
33245474	8	68	from	N-glycans	1181:1189	arg1	ACE2					1234:1237	its main receptor ACE2	1216:1237	its main receptor ACE2	1216:1237	In our overexpression system, miglustat successfully and specifically modified N-glycans in both SARS-CoV-2 S and its main receptor ACE2.					
33245474	8	68	from	N-glycans	1181:1189	arg1	S					1210:1210	SARS-CoV-2 S	1199:1210	SARS-CoV-2 S	1199:1210	In our overexpression system, miglustat successfully and specifically modified N-glycans in both SARS-CoV-2 S and its main receptor ACE2.					
33245474	0	69	from	Modification	9:20	arg1	Pathophysiology					34:48	Covid-19 Pathophysiology	25:48	Covid-19 Pathophysiology	25:48	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.					
33245474	12	70	theme	glycoprotein	1646:1657	arg1	stimulation					1659:1669	SARS-CoV-2 S glycoprotein stimulation	1633:1669	SARS-CoV-2 S glycoprotein stimulation of PBMC	1633:1677	Miglustat produced no statistically significant effects on cytokine production following SARS-CoV-2 S glycoprotein stimulation of PBMC.					
33245474	10	71	theme	cytopathic	1340:1349	arg1	effect					1351:1356	viral cytopathic effect	1334:1356	viral cytopathic effect	1334:1356	A surrogate marker for viral cytopathic effect, measured as receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion, was not disrupted by miglustat treatment.					
33245474	7	72	theme	immune	1001:1006	arg1	response					1008:1015	immune response	1001:1015	immune response	1001:1015	Miglustat effect on immune response was tested by measuring cytokine release from PBMC exposed to purified SARS-CoV-2 S.					
33245474	2	73	dep	enveloped	367:375	arg1	N-glycosylation-dependent					378:402	N-glycosylation-dependent	378:402	N-glycosylation-dependent	378:402	The glycan alteration derived from this disease resulted in an in vitro infection resistance to particular enveloped, N-glycosylation-dependent viruses as influenza and HIV.					
33245474	3	74	theme	global	449:454	arg1	effort					456:461	the global effort to find safe and effective antiviral therapies for Covid-19	445:521	the global effort to find safe and effective antiviral therapies for Covid-19	445:521	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	8	75	theme	overexpression	1109:1122	arg1	system					1124:1129	our overexpression system	1105:1129	our overexpression system	1105:1129	In our overexpression system, miglustat successfully and specifically modified N-glycans in both SARS-CoV-2 S and its main receptor ACE2.					
33245474	13	76	theme	glycoproteins	1802:1814	arg1	functions					1768:1776	the functions	1764:1776	the functions of the Covid-19-related glycoproteins	1764:1814	Our work shows that despite clear N-glycan alteration in the presence of miglustat, the functions of the Covid-19-related glycoproteins studied were not affected, making it unlikely that miglustat can change the natural course of the disease.					
33245474	0	77	theme	Covid-19	25:32	arg1	Pathophysiology					34:48	Covid-19 Pathophysiology	25:48	Covid-19 Pathophysiology	25:48	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.					
33245474	11	78	theme	transfected	1525:1535	arg1	cells					1537:1541	MOGS-null transfected cells	1515:1541	MOGS-null transfected cells	1515:1541	This observation was further confirmed in MOGS-null transfected cells.					
33245474	3	79	theme	safe	471:474	arg1	therapies					500:508	safe and effective antiviral therapies	471:508	safe and effective antiviral therapies for Covid-19	471:521	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	11	80	theme	MOGS-null	1515:1523	arg1	cells					1537:1541	MOGS-null transfected cells	1515:1541	MOGS-null transfected cells	1515:1541	This observation was further confirmed in MOGS-null transfected cells.					
33245474	10	81	theme	cell-to-cell	1410:1421	arg1	fusion					1423:1428	receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion	1371:1428	receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion	1371:1428	A surrogate marker for viral cytopathic effect, measured as receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion, was not disrupted by miglustat treatment.					
33245474	12	82	theme	cytokine	1603:1610	arg1	production					1612:1621	cytokine production	1603:1621	cytokine production	1603:1621	Miglustat produced no statistically significant effects on cytokine production following SARS-CoV-2 S glycoprotein stimulation of PBMC.					
33245474	10	83	theme	SARS-CoV-2	1390:1399	arg1	fusion					1423:1428	receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion	1371:1428	receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion	1371:1428	A surrogate marker for viral cytopathic effect, measured as receptor-dependent SARS-CoV-2 S-driven cell-to-cell fusion, was not disrupted by miglustat treatment.					
33245474	2	84	theme	vitro infection	326:340	arg1	resistance					342:351	vitro infection resistance	326:351	vitro infection resistance to particular enveloped, N-glycosylation-dependent viruses as influenza and HIV	326:431	The glycan alteration derived from this disease resulted in an in vitro infection resistance to particular enveloped, N-glycosylation-dependent viruses as influenza and HIV.					
33245474	3	85	theme	effective	480:488	arg1	therapies					500:508	safe and effective antiviral therapies	471:508	safe and effective antiviral therapies for Covid-19	471:521	As part of the global effort to find safe and effective antiviral therapies for Covid-19, we assessed the in vitro activity of the FDA-approved α-glucosidase inhibitor miglustat against SARS-CoV-2.					
33245474	1	86	theme	mannosyl-oligosaccharide	215:238	arg1	MOGS					253:256	mannosyl-oligosaccharide glucosidase (MOGS)	215:257	mannosyl-oligosaccharide glucosidase (MOGS)	215:257	In 2014, we reported two siblings with a rare congenital disorder of glycosylation due to mutations in mannosyl-oligosaccharide glucosidase (MOGS).					
33245474	0	87	dep	Modification	9:20	arg1	Changes					71:77	In vitro Structural Changes	51:77	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.	0:110	N-Glycan Modification in Covid-19 Pathophysiology: In vitro Structural Changes with Limited Functional Effects.					
33245474	2	88	theme	enveloped	367:375	arg1	viruses					404:410	particular enveloped, N-glycosylation-dependent viruses	356:410	particular enveloped, N-glycosylation-dependent viruses as influenza and HIV	356:431	The glycan alteration derived from this disease resulted in an in vitro infection resistance to particular enveloped, N-glycosylation-dependent viruses as influenza and HIV.					
34127537	7	0	dep	RESULTS	1017:1023	arg1	features					1045:1052	Multiple structural features	1025:1052	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2	1017:1088	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	11	1	theme	future	1638:1643	arg1	investigations					1645:1658	future investigations	1638:1658	future investigations on the pathophysiology of IgAN	1638:1689	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	6	2	from	controls	1007:1014	arg1	analysis					896:903	the analysis	892:903	the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls	892:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	2	from	controls	1007:1014	arg1	glycopeptides					916:928	tryptic glycopeptides	908:928	tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls	908:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	2	3	theme	galactose-deficient	320:338	arg1	IgA					340:342	galactose-deficient IgA	320:342	galactose-deficient IgA	320:342	The disease mechanisms are not completely understood, but a higher abundance of galactose-deficient IgA is recognized to play a crucial role in IgAN pathogenesis.					
34127537	1	4	theme	failure	231:237	arg1	cause					216:220	a leading cause	206:220	a leading cause of renal failure	206:237	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	1	4	theme	failure	231:237	arg1	worldwide					189:197	the most common primary glomerular disease worldwide	146:197	the most common primary glomerular disease worldwide	146:197	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	1	4	theme	failure	231:237	arg1	nephropathy					124:134	BACKGROUND IgA nephropathy	109:134	BACKGROUND IgA nephropathy (IgAN)	109:141	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	6	5	theme	chromatography-mass	847:865	arg1	spectrometry					867:878	liquid chromatography-mass spectrometry	840:878	liquid chromatography-mass spectrometry (LC-MS)	840:886	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	5	theme	chromatography-mass	847:865	arg1	LC-MS					881:885	LC-MS	881:885	LC-MS	881:885	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	4	6	theme	galactose	575:583	arg1	deficiency					585:594	IgA1 galactose deficiency	570:594	IgA1 galactose deficiency on the O-glycans	570:611	IgA1 galactose deficiency on the O-glycans is commonly detected by a lectin-based method.					
34127537	11	7	theme	IgAN	1686:1689	arg1	pathophysiology					1667:1681	the pathophysiology	1663:1681	the pathophysiology of IgAN	1663:1689	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	6	8	theme	complex	769:775	arg1	O-					777:778	complex O-	769:778	complex O-	769:778	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	4	9	theme	lectin-based	639:650	arg1	method					652:657	a lectin-based method	637:657	a lectin-based method	637:657	IgA1 galactose deficiency on the O-glycans is commonly detected by a lectin-based method.					
34127537	6	10	gly	glycopeptides	916:928	arg1	controls					1007:1014	244 age- and sex-matched healthy controls	974:1014	244 age- and sex-matched healthy controls	974:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	10	gly	glycopeptides	916:928	arg2	glycopeptides					916:928	tryptic glycopeptides	908:928	tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls	908:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	10	gly	glycopeptides	916:928	arg1	IgA					939:941	serum IgA	933:941	serum IgA	933:941	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	10	gly	glycopeptides	916:928	arg1	patients					951:958	83 patients	948:958	83 patients with IgAN	948:968	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	2	11	theme	disease	244:250	arg1	mechanisms					252:261	The disease mechanisms	240:261	The disease mechanisms	240:261	The disease mechanisms are not completely understood, but a higher abundance of galactose-deficient IgA is recognized to play a crucial role in IgAN pathogenesis.					
34127537	1	12	theme	common	155:160	arg1	nephropathy					124:134	BACKGROUND IgA nephropathy	109:134	BACKGROUND IgA nephropathy (IgAN)	109:141	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	1	12	theme	common	155:160	arg1	worldwide					189:197	the most common primary glomerular disease worldwide	146:197	the most common primary glomerular disease worldwide	146:197	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	7	13	theme	structural	1034:1043	arg1	features					1045:1052	Multiple structural features	1025:1052	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2	1017:1088	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	11	14	theme	high-resolution	1551:1565	arg1	data					1567:1570	Our high-resolution data	1547:1570	Our high-resolution data	1547:1570	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	3	15	theme	hinge-region	540:551	arg1	O-glycosylation					553:567	extensive hinge-region O-glycosylation	530:567	extensive hinge-region O-glycosylation	530:567	Although both types of human IgA (IgA1 and IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation.					
34127537	1	16	theme	glomerular	170:179	arg1	nephropathy					124:134	BACKGROUND IgA nephropathy	109:134	BACKGROUND IgA nephropathy (IgAN)	109:141	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	1	16	theme	glomerular	170:179	arg1	worldwide					189:197	the most common primary glomerular disease worldwide	146:197	the most common primary glomerular disease worldwide	146:197	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	6	17	theme	glycopeptides	916:928	arg1	analysis					896:903	the analysis	892:903	the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls	892:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	3	18	theme	several	457:463	arg1	N-glycans					465:473	several N-glycans	457:473	several N-glycans	457:473	Although both types of human IgA (IgA1 and IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation.					
34127537	6	19	theme	healthy	999:1005	arg1	controls					1007:1014	244 age- and sex-matched healthy controls	974:1014	244 age- and sex-matched healthy controls	974:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	20	from	N-glycosylation	784:798	arg1	IgAN					826:829	IgAN	826:829	IgAN	826:829	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	1	21	theme	IgA	120:122	arg1	nephropathy					124:134	BACKGROUND IgA nephropathy	109:134	BACKGROUND IgA nephropathy (IgAN)	109:141	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	1	21	theme	IgA	120:122	arg1	worldwide					189:197	the most common primary glomerular disease worldwide	146:197	the most common primary glomerular disease worldwide	146:197	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	1	21	theme	IgA	120:122	arg1	IgAN					137:140	IgAN	137:140	IgAN	137:140	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	8	22	theme	N-glycan	1268:1275	arg1	differences					1194:1204	differences	1194:1204	differences	1194:1204	These features included differences in galactosylation, sialylation, bisection, fucosylation, and N-glycan complexity.					
34127537	8	22	theme	N-glycan	1268:1275	arg1	complexity					1277:1286	N-glycan complexity	1268:1286	N-glycan complexity	1268:1286	These features included differences in galactosylation, sialylation, bisection, fucosylation, and N-glycan complexity.					
34127537	5	23	theme	limited	669:675	arg1	detail					677:682	limited detail	669:682	limited detail	669:682	To date, limited detail is known about IgA O- and N-glycosylation in IgAN.					
34127537	11	24	theme	deteriorating	1758:1770	arg1	function					1779:1786	deteriorating kidney function	1758:1786	deteriorating kidney function	1758:1786	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	3	25	contain	have	452:455	arg1	types					417:421	both types	412:421	both types of human IgA (IgA1 and IgA2)	412:450	Although both types of human IgA (IgA1 and IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation.					
34127537	3	25	contain	have	452:455	arg2	N-glycans					465:473	several N-glycans	457:473	several N-glycans	457:473	Although both types of human IgA (IgA1 and IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation.					
34127537	0	26	theme	IgA	68:70	arg1	Nephropathy					72:82	IgA Nephropathy	68:82	IgA Nephropathy	68:82	O- and N-Glycosylation of Serum Immunoglobulin A is Associated with IgA Nephropathy and Glomerular Function.					
34127537	6	27	theme	serum	933:937	arg1	IgA					939:941	serum IgA	933:941	serum IgA	933:941	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	10	28	theme	glomerular	1446:1455	arg1	function					1457:1464	glomerular function	1446:1464	glomerular function	1446:1464	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	2	29	theme	crucial	368:374	arg1	role					376:379	a crucial role	366:379	a crucial role	366:379	The disease mechanisms are not completely understood, but a higher abundance of galactose-deficient IgA is recognized to play a crucial role in IgAN pathogenesis.					
34127537	11	30	gly	N-glycopeptides	1596:1610	arg2	IgA					1585:1587	IgA O- and N-glycopeptides	1585:1610	IgA	1585:1587	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	11	30	gly	N-glycopeptides	1596:1610	arg2	targets					1626:1632	promising targets	1616:1632	promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function	1616:1786	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	11	30	gly	N-glycopeptides	1596:1610	arg2	N-glycopeptides					1596:1610	N-glycopeptides	1596:1610	N-glycopeptides	1596:1610	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	7	31	from	function	1131:1138	arg1	study					1163:1167	our cross-sectional study	1143:1167	our cross-sectional study	1143:1167	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	10	32	theme	IgAN	1437:1440	arg1	predictor					1424:1432	a better predictor	1415:1432	a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA	1415:1532	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	10	32	theme	IgAN	1437:1440	arg1	glycopeptides					1396:1408	glycopeptides	1396:1408	glycopeptides	1396:1408	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	9	33	theme	IgA1	1299:1302	arg1	sialylation					1313:1323	IgA1 O-glycan sialylation	1299:1323	IgA1 O-glycan sialylation	1299:1323	Moreover, IgA1 O-glycan sialylation was associated with both the disease and glomerular function.					
34127537	6	34	from	O-	777:778	arg1	IgAN					826:829	IgAN	826:829	IgAN	826:829	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	10	35	theme	IgA1	1491:1494	arg1	levels					1496:1501	galactose-deficient IgA1 levels	1471:1501	galactose-deficient IgA1 levels measured by lectin-based ELISA	1471:1532	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	5	36	gly	N-glycosylation	710:724	arg1	IgAN					729:732	IgAN	729:732	IgAN	729:732	To date, limited detail is known about IgA O- and N-glycosylation in IgAN.					
34127537	3	37	theme	IgA	432:434	arg1	types					417:421	both types	412:421	both types of human IgA (IgA1 and IgA2)	412:450	Although both types of human IgA (IgA1 and IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation.					
34127537	6	38	with	controls	1007:1014	arg1	IgAN					965:968	IgAN	965:968	IgAN	965:968	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	0	39	theme	Serum	26:30	arg1	A					47:47	Serum Immunoglobulin A	26:47	Serum Immunoglobulin A	26:47	O- and N-Glycosylation of Serum Immunoglobulin A is Associated with IgA Nephropathy and Glomerular Function.					
34127537	6	40	theme	IgA2	818:821	arg1	N-glycosylation					784:798	N-glycosylation	784:798	N-glycosylation	784:798	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	40	theme	IgA2	818:821	arg1	O-					777:778	complex O-	769:778	complex O-	769:778	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	41	theme	244	974:976	arg1	age-					978:981	age-	978:981	age-	978:981	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	10	42	theme	lectin-based	1515:1526	arg1	ELISA					1528:1532	lectin-based ELISA	1515:1532	lectin-based ELISA	1515:1532	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	11	43	theme	potential	1698:1706	arg1	biomarkers					1720:1729	potential noninvasive biomarkers	1698:1729	potential noninvasive biomarkers for disease prediction and deteriorating kidney function	1698:1786	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	0	44	theme	A	47:47	arg1	N-Glycosylation					7:21	N-Glycosylation	7:21	N-Glycosylation	7:21	O- and N-Glycosylation of Serum Immunoglobulin A is Associated with IgA Nephropathy and Glomerular Function.					
34127537	0	44	theme	A	47:47	arg1	O-					0:1	O-	0:1	O-	0:1	O- and N-Glycosylation of Serum Immunoglobulin A is Associated with IgA Nephropathy and Glomerular Function.					
34127537	6	45	theme	IgA1	809:812	arg1	N-glycosylation					784:798	N-glycosylation	784:798	N-glycosylation	784:798	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	45	theme	IgA1	809:812	arg1	O-					777:778	complex O-	769:778	complex O-	769:778	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	46	from	analysis	896:903	arg1	controls					1007:1014	244 age- and sex-matched healthy controls	974:1014	244 age- and sex-matched healthy controls	974:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	46	from	analysis	896:903	arg1	patients					951:958	83 patients	948:958	83 patients with IgAN	948:968	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	2	47	theme	higher	300:305	arg1	abundance					307:315	a higher abundance	298:315	a higher abundance of galactose-deficient IgA	298:342	The disease mechanisms are not completely understood, but a higher abundance of galactose-deficient IgA is recognized to play a crucial role in IgAN pathogenesis.					
34127537	11	48	dep	CONCLUSIONS	1535:1545	arg1	suggest					1572:1578	suggest	1572:1578	suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function	1572:1786	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	11	49	theme	disease	1735:1741	arg1	prediction					1743:1752	disease prediction	1735:1752	disease prediction	1735:1752	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	9	50	theme	glomerular	1366:1375	arg1	function					1377:1384	glomerular function	1366:1384	glomerular function	1366:1384	Moreover, IgA1 O-glycan sialylation was associated with both the disease and glomerular function.					
34127537	7	51	theme	IgA1	1076:1079	arg1	N-glycosylation					1057:1071	N-glycosylation	1057:1071	N-glycosylation of IgA1 and IgA2	1057:1088	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	11	52	theme	promising	1616:1624	arg1	IgA					1585:1587	IgA O- and N-glycopeptides	1585:1610	IgA	1585:1587	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	11	52	theme	promising	1616:1624	arg1	N-glycopeptides					1596:1610	N-glycopeptides	1596:1610	N-glycopeptides	1596:1610	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	11	52	theme	promising	1616:1624	arg1	targets					1626:1632	promising targets	1616:1632	promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function	1616:1786	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	2	53	theme	IgA	340:342	arg1	abundance					307:315	a higher abundance	298:315	a higher abundance of galactose-deficient IgA	298:342	The disease mechanisms are not completely understood, but a higher abundance of galactose-deficient IgA is recognized to play a crucial role in IgAN pathogenesis.					
34127537	1	54	theme	renal	225:229	arg1	failure					231:237	renal failure	225:237	renal failure	225:237	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	7	55	theme	N-glycosylation	1057:1071	arg1	features					1045:1052	Multiple structural features	1025:1052	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2	1017:1088	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	6	56	theme	liquid	840:845	arg1	spectrometry					867:878	liquid chromatography-mass spectrometry	840:878	liquid chromatography-mass spectrometry (LC-MS)	840:886	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	56	theme	liquid	840:845	arg1	LC-MS					881:885	LC-MS	881:885	LC-MS	881:885	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	11	57	dep	IgA	1585:1587	arg1	O-					1589:1590	O-	1589:1590	O-	1589:1590	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	4	58	theme	IgA1	570:573	arg1	deficiency					585:594	IgA1 galactose deficiency	570:594	IgA1 galactose deficiency on the O-glycans	570:611	IgA1 galactose deficiency on the O-glycans is commonly detected by a lectin-based method.					
34127537	3	59	theme	post-translational	478:495	arg1	modification					497:508	post-translational modification	478:508	post-translational modification	478:508	Although both types of human IgA (IgA1 and IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation.					
34127537	7	60	theme	cross-sectional	1147:1161	arg1	study					1163:1167	our cross-sectional study	1143:1167	our cross-sectional study	1143:1167	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	6	61	from	IgA2	818:821	arg1	IgAN					826:829	IgAN	826:829	IgAN	826:829	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	4	62	from	deficiency	585:594	arg1	O-glycans					603:611	the O-glycans	599:611	the O-glycans	599:611	IgA1 galactose deficiency on the O-glycans is commonly detected by a lectin-based method.					
34127537	7	63	theme	Multiple	1025:1032	arg1	features					1045:1052	Multiple structural features	1025:1052	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2	1017:1088	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	3	64	theme	extensive	530:538	arg1	O-glycosylation					553:567	extensive hinge-region O-glycosylation	530:567	extensive hinge-region O-glycosylation	530:567	Although both types of human IgA (IgA1 and IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation.					
34127537	1	65	theme	primary	162:168	arg1	nephropathy					124:134	BACKGROUND IgA nephropathy	109:134	BACKGROUND IgA nephropathy (IgAN)	109:141	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	1	65	theme	primary	162:168	arg1	worldwide					189:197	the most common primary glomerular disease worldwide	146:197	the most common primary glomerular disease worldwide	146:197	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	10	66	theme	better	1417:1422	arg1	predictor					1424:1432	a better predictor	1415:1432	a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA	1415:1532	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	10	66	theme	better	1417:1422	arg1	glycopeptides					1396:1408	glycopeptides	1396:1408	glycopeptides	1396:1408	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	6	67	from	IgAN	826:829	arg1	N-glycosylation					784:798	N-glycosylation	784:798	N-glycosylation	784:798	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	67	from	IgAN	826:829	arg1	O-					777:778	complex O-	769:778	complex O-	769:778	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	1	68	theme	disease	181:187	arg1	nephropathy					124:134	BACKGROUND IgA nephropathy	109:134	BACKGROUND IgA nephropathy (IgAN)	109:141	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	1	68	theme	disease	181:187	arg1	worldwide					189:197	the most common primary glomerular disease worldwide	146:197	the most common primary glomerular disease worldwide	146:197	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	6	69	theme	tryptic	908:914	arg1	glycopeptides					916:928	tryptic glycopeptides	908:928	tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls	908:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	5	70	from	N-glycosylation	710:724	arg1	IgAN					729:732	IgAN	729:732	IgAN	729:732	To date, limited detail is known about IgA O- and N-glycosylation in IgAN.					
34127537	6	71	with	patients	951:958	arg1	IgAN					965:968	IgAN	965:968	IgAN	965:968	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	7	72	gly	N-glycosylation	1057:1071	arg1	IgA2					1085:1088	IgA2	1085:1088	IgA2	1085:1088	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	7	72	gly	N-glycosylation	1057:1071	arg1	IgA1					1076:1079	IgA1	1076:1079	IgA1	1076:1079	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	6	73	from	IgA1	809:812	arg1	IgAN					826:829	IgAN	826:829	IgAN	826:829	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	5	74	theme	IgA	699:701	arg1	O-					703:704	IgA O-	699:704	IgA O-	699:704	To date, limited detail is known about IgA O- and N-glycosylation in IgAN.					
34127537	5	75	from	O-	703:704	arg1	IgAN					729:732	IgAN	729:732	IgAN	729:732	To date, limited detail is known about IgA O- and N-glycosylation in IgAN.					
34127537	1	76	theme	BACKGROUND	109:118	arg1	nephropathy					124:134	BACKGROUND IgA nephropathy	109:134	BACKGROUND IgA nephropathy (IgAN)	109:141	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	1	76	theme	BACKGROUND	109:118	arg1	worldwide					189:197	the most common primary glomerular disease worldwide	146:197	the most common primary glomerular disease worldwide	146:197	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	1	76	theme	BACKGROUND	109:118	arg1	IgAN					137:140	IgAN	137:140	IgAN	137:140	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	8	77	from	differences	1194:1204	arg1	galactosylation					1209:1223	galactosylation	1209:1223	galactosylation	1209:1223	These features included differences in galactosylation, sialylation, bisection, fucosylation, and N-glycan complexity.					
34127537	11	78	theme	kidney	1772:1777	arg1	function					1779:1786	deteriorating kidney function	1758:1786	deteriorating kidney function	1758:1786	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	6	79	used	used	835:838	arg2	we					832:833	we	832:833	we	832:833	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	80	from	patients	951:958	arg1	analysis					896:903	the analysis	892:903	the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls	892:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	80	from	patients	951:958	arg1	glycopeptides					916:928	tryptic glycopeptides	908:928	tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls	908:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	0	81	theme	Glomerular	88:97	arg1	Function					99:106	Glomerular Function	88:106	Glomerular Function	88:106	O- and N-Glycosylation of Serum Immunoglobulin A is Associated with IgA Nephropathy and Glomerular Function.					
34127537	6	82	theme	IgA	939:941	arg1	glycopeptides					916:928	tryptic glycopeptides	908:928	tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls	908:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	7	83	from	IgAN	1111:1114	arg1	study					1163:1167	our cross-sectional study	1143:1167	our cross-sectional study	1143:1167	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	6	84	gly	N-glycosylation	784:798	arg1	IgA2					818:821	IgA2	818:821	IgA2	818:821	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	6	84	gly	N-glycosylation	784:798	arg1	IgA1					809:812	serum IgA1	803:812	serum IgA1	803:812	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	7	85	theme	glomerular	1120:1129	arg1	function					1131:1138	glomerular function	1120:1138	glomerular function	1120:1138	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	10	86	gly	glycopeptides	1396:1408	arg2	glycopeptides					1396:1408	glycopeptides	1396:1408	glycopeptides	1396:1408	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	10	86	gly	glycopeptides	1396:1408	arg2	predictor					1424:1432	a better predictor	1415:1432	a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA	1415:1532	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	9	87	theme	O-glycan	1304:1311	arg1	sialylation					1313:1323	IgA1 O-glycan sialylation	1299:1323	IgA1 O-glycan sialylation	1299:1323	Moreover, IgA1 O-glycan sialylation was associated with both the disease and glomerular function.					
34127537	6	88	theme	sex-matched	987:997	arg1	controls					1007:1014	244 age- and sex-matched healthy controls	974:1014	244 age- and sex-matched healthy controls	974:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	10	89	theme	galactose-deficient	1471:1489	arg1	levels					1496:1501	galactose-deficient IgA1 levels	1471:1501	galactose-deficient IgA1 levels measured by lectin-based ELISA	1471:1532	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	11	90	theme	noninvasive	1708:1718	arg1	biomarkers					1720:1729	potential noninvasive biomarkers	1698:1729	potential noninvasive biomarkers for disease prediction and deteriorating kidney function	1698:1786	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	6	91	dep	O-	777:778	arg1	the					765:767	the	765:767	the	765:767	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	2	92	theme	IgAN	384:387	arg1	pathogenesis					389:400	IgAN pathogenesis	384:400	IgAN pathogenesis	384:400	The disease mechanisms are not completely understood, but a higher abundance of galactose-deficient IgA is recognized to play a crucial role in IgAN pathogenesis.					
34127537	0	93	theme	Immunoglobulin	32:45	arg1	A					47:47	Serum Immunoglobulin A	26:47	Serum Immunoglobulin A	26:47	O- and N-Glycosylation of Serum Immunoglobulin A is Associated with IgA Nephropathy and Glomerular Function.					
34127537	6	94	theme	age-	978:981	arg1	controls					1007:1014	244 age- and sex-matched healthy controls	974:1014	244 age- and sex-matched healthy controls	974:1014	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	11	95	from	investigations	1645:1658	arg1	pathophysiology					1667:1681	the pathophysiology	1663:1681	the pathophysiology of IgAN	1663:1689	CONCLUSIONS Our high-resolution data suggest that IgA O- and N-glycopeptides are promising targets for future investigations on the pathophysiology of IgAN and as potential noninvasive biomarkers for disease prediction and deteriorating kidney function.					
34127537	10	96	theme	function	1457:1464	arg1	predictor					1424:1432	a better predictor	1415:1432	a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA	1415:1532	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	10	96	theme	function	1457:1464	arg1	glycopeptides					1396:1408	glycopeptides	1396:1408	glycopeptides	1396:1408	Finally, glycopeptides were a better predictor of IgAN and glomerular function than galactose-deficient IgA1 levels measured by lectin-based ELISA.					
34127537	3	97	theme	human	426:430	arg1	IgA					432:434	human IgA	426:434	human IgA (IgA1 and IgA2)	426:450	Although both types of human IgA (IgA1 and IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation.					
34127537	7	98	theme	IgA2	1085:1088	arg1	N-glycosylation					1057:1071	N-glycosylation	1057:1071	N-glycosylation of IgA1 and IgA2	1057:1088	RESULTS Multiple structural features of N-glycosylation of IgA1 and IgA2 were associated with IgAN and glomerular function in our cross-sectional study.					
34127537	0	99	gly	N-Glycosylation	7:21	arg1	A					47:47	Serum Immunoglobulin A	26:47	Serum Immunoglobulin A	26:47	O- and N-Glycosylation of Serum Immunoglobulin A is Associated with IgA Nephropathy and Glomerular Function.					
34127537	6	100	theme	serum	803:807	arg1	IgA1					809:812	serum IgA1	803:812	serum IgA1	803:812	METHODS To gain insights into the complex O- and N-glycosylation of serum IgA1 and IgA2 in IgAN, we used liquid chromatography-mass spectrometry (LC-MS) for the analysis of tryptic glycopeptides of serum IgA from 83 patients with IgAN and 244 age- and sex-matched healthy controls.					
34127537	1	101	theme	leading	208:214	arg1	cause					216:220	a leading cause	206:220	a leading cause of renal failure	206:237	BACKGROUND IgA nephropathy (IgAN) is the most common primary glomerular disease worldwide and is a leading cause of renal failure.					
34127537	3	102	dep	IgA	432:434	arg1	IgA2					446:449	IgA2	446:449	IgA2	446:449	Although both types of human IgA (IgA1 and IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation.					
34127537	3	102	dep	IgA	432:434	arg1	IgA1					437:440	IgA1	437:440	IgA1	437:440	Although both types of human IgA (IgA1 and IgA2) have several N-glycans as post-translational modification, only IgA1 features extensive hinge-region O-glycosylation.					
32248235	0	0	theme	glycan	102:107	arg1	electrophoresis					109:123	glycan electrophoresis	102:123	glycan electrophoresis	102:123	Detecting substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis.					
32248235	7	1	theme	X	1275:1275	arg1	structures					1296:1305	Lewis X and sialyl Lewis X structures	1269:1305	Lewis X and sialyl Lewis X structures	1269:1305	Our results support the notion that fucosyltransferases are key targets for regulating the synthesis of Lewis X and sialyl Lewis X structures.					
32248235	4	2	theme	enzymes	729:735	arg1	glycans					712:718	the substrate glycans	698:718	the substrate glycans of these enzymes	698:735	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	0	3	with	fucosyltransferases	31:49	arg1	fucose					79:84	fluorophore-conjugated fucose	56:84	fluorophore-conjugated fucose	56:84	Detecting substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis.					
32248235	1	4	theme	nonreducing	174:184	arg1	ends					186:189	the nonreducing ends	170:189	the nonreducing ends of various glycans on glycoproteins	170:225	Like sialylation, fucose usually locates at the nonreducing ends of various glycans on glycoproteins and constitutes important glycan epitopes.					
32248235	6	5	theme	X	957:957	arg1	structures					978:987	Lewis X and sialyl Lewis X structures	951:987	Lewis X and sialyl Lewis X structures	951:987	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	7	6	theme	structures	1296:1305	arg1	synthesis					1256:1264	the synthesis	1252:1264	the synthesis of Lewis X and sialyl Lewis X structures	1252:1305	Our results support the notion that fucosyltransferases are key targets for regulating the synthesis of Lewis X and sialyl Lewis X structures.					
32248235	6	7	theme	Lewis	951:955	arg1	X					957:957	Lewis X	951:957	Lewis X	951:957	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	1	8	from	ends	186:189	arg1	glycoproteins					213:225	glycoproteins	213:225	glycoproteins	213:225	Like sialylation, fucose usually locates at the nonreducing ends of various glycans on glycoproteins and constitutes important glycan epitopes.					
32248235	3	9	theme	fluorophore-conjugated	586:607	arg1	fucose					609:614	fluorophore-conjugated fucose	586:614	fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9	586:652	Here we report the detection of these glycans on glycoproteins as well as in their free forms via enzymatic incorporation of fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9.					
32248235	6	10	theme	electrophoresis	1021:1035	arg1	methods					1037:1043	convenient electrophoresis methods	1010:1043	convenient electrophoresis methods for studying glycosylation	1010:1070	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	4	11	theme	recombinant	761:771	arg1	neuraminidase					784:796	recombinant H1N1 viral neuraminidase	761:796	recombinant H1N1 viral neuraminidase	761:796	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	0	12	with	methods	90:96	arg1	fucose					79:84	fluorophore-conjugated fucose	56:84	fluorophore-conjugated fucose	56:84	Detecting substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis.					
32248235	3	13	theme	fucose	609:614	arg1	incorporation					569:581	enzymatic incorporation	559:581	enzymatic incorporation of fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9	559:652	Here we report the detection of these glycans on glycoproteins as well as in their free forms via enzymatic incorporation of fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9.					
32248235	4	14	theme	bovine	746:751	arg1	fetuin					753:758	fetal bovine fetuin	740:758	fetal bovine fetuin	740:758	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	4	15	theme	substrate	702:710	arg1	glycans					712:718	the substrate glycans	698:718	the substrate glycans of these enzymes	698:735	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	6	16	theme	convenient	1010:1019	arg1	methods					1037:1043	convenient electrophoresis methods	1010:1043	convenient electrophoresis methods for studying glycosylation	1010:1070	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	1	17	gly	glycoproteins	213:225	arg1	glycoproteins					213:225	glycoproteins	213:225	glycoproteins	213:225	Like sialylation, fucose usually locates at the nonreducing ends of various glycans on glycoproteins and constitutes important glycan epitopes.					
32248235	2	18	theme	fucosyltransferases	305:323	arg1	glycans					294:300	the substrate glycans	280:300	the substrate glycans of fucosyltransferases	280:323	Detecting the substrate glycans of fucosyltransferases is important for understanding how these glycan epitopes are regulated in response to different growth conditions and external stimuli.					
32248235	1	19	theme	various	194:200	arg1	glycans					202:208	various glycans	194:208	various glycans on glycoproteins	194:225	Like sialylation, fucose usually locates at the nonreducing ends of various glycans on glycoproteins and constitutes important glycan epitopes.					
32248235	0	20	theme	substrate	10:18	arg1	glycans					20:26	substrate glycans	10:26	substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis	10:123	Detecting substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis.					
32248235	7	21	theme	sialyl	1281:1286	arg1	X					1294:1294	sialyl Lewis X	1281:1294	sialyl Lewis X	1281:1294	Our results support the notion that fucosyltransferases are key targets for regulating the synthesis of Lewis X and sialyl Lewis X structures.					
32248235	2	22	theme	glycan	366:371	arg1	epitopes					373:380	these glycan epitopes	360:380	these glycan epitopes	360:380	Detecting the substrate glycans of fucosyltransferases is important for understanding how these glycan epitopes are regulated in response to different growth conditions and external stimuli.					
32248235	4	23	from	detection	685:693	arg1	antibodies					814:823	therapeutic antibodies	802:823	therapeutic antibodies	802:823	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	4	23	from	detection	685:693	arg1	neuraminidase					784:796	recombinant H1N1 viral neuraminidase	761:796	recombinant H1N1 viral neuraminidase	761:796	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	4	23	from	detection	685:693	arg1	fetuin					753:758	fetal bovine fetuin	740:758	fetal bovine fetuin	740:758	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	1	24	theme	glycans	202:208	arg1	ends					186:189	the nonreducing ends	170:189	the nonreducing ends of various glycans on glycoproteins	170:225	Like sialylation, fucose usually locates at the nonreducing ends of various glycans on glycoproteins and constitutes important glycan epitopes.					
32248235	5	25	theme	complex	855:861	arg1	N-glycans					880:888	complex and high-mannose N-glycans	855:888	complex and high-mannose N-glycans	855:888	The detected glycans include complex and high-mannose N-glycans.					
32248235	7	26	theme	key	1225:1227	arg1	fucosyltransferases					1201:1219	fucosyltransferases	1201:1219	fucosyltransferases	1201:1219	Our results support the notion that fucosyltransferases are key targets for regulating the synthesis of Lewis X and sialyl Lewis X structures.					
32248235	7	26	theme	key	1225:1227	arg1	targets					1229:1235	key targets	1225:1235	key targets for regulating the synthesis of Lewis X and sialyl Lewis X structures	1225:1305	Our results support the notion that fucosyltransferases are key targets for regulating the synthesis of Lewis X and sialyl Lewis X structures.					
32248235	6	27	theme	kinetic	1130:1136	arg1	features					1138:1145	some kinetic features	1125:1145	some kinetic features	1125:1145	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	4	28	theme	viral	778:782	arg1	neuraminidase					784:796	recombinant H1N1 viral neuraminidase	761:796	recombinant H1N1 viral neuraminidase	761:796	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	5	29	theme	high-mannose	867:878	arg1	N-glycans					880:888	complex and high-mannose N-glycans	855:888	complex and high-mannose N-glycans	855:888	The detected glycans include complex and high-mannose N-glycans.					
32248235	6	30	theme	precursors	919:928	arg1	series					909:914	a series	907:914	a series of precursors	907:928	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	4	31	theme	H1N1	773:776	arg1	neuraminidase					784:796	recombinant H1N1 viral neuraminidase	761:796	recombinant H1N1 viral neuraminidase	761:796	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	6	32	theme	substrate	1097:1105	arg1	specificities					1107:1119	the substrate specificities	1093:1119	the substrate specificities	1093:1119	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	3	33	theme	free	544:547	arg1	forms					549:553	their free forms	538:553	their free forms	538:553	Here we report the detection of these glycans on glycoproteins as well as in their free forms via enzymatic incorporation of fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9.					
32248235	3	34	theme	glycans	499:505	arg1	detection					480:488	the detection	476:488	the detection of these glycans on glycoproteins as well as in their free forms	476:553	Here we report the detection of these glycans on glycoproteins as well as in their free forms via enzymatic incorporation of fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9.					
32248235	0	35	theme	fucosyltransferases	31:49	arg1	glycans					20:26	substrate glycans	10:26	substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis	10:123	Detecting substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis.					
32248235	3	36	gly	glycoproteins	510:522	arg1	glycoproteins					510:522	glycoproteins	510:522	glycoproteins	510:522	Here we report the detection of these glycans on glycoproteins as well as in their free forms via enzymatic incorporation of fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9.					
32248235	1	37	theme	important	243:251	arg1	epitopes					260:267	important glycan epitopes	243:267	important glycan epitopes	243:267	Like sialylation, fucose usually locates at the nonreducing ends of various glycans on glycoproteins and constitutes important glycan epitopes.					
32248235	5	38	theme	detected	830:837	arg1	glycans					839:845	The detected glycans	826:845	The detected glycans	826:845	The detected glycans include complex and high-mannose N-glycans.					
32248235	6	39	theme	structures	978:987	arg1	synthesis					938:946	the synthesis	934:946	the synthesis of Lewis X and sialyl Lewis X structures	934:987	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	4	40	theme	fetal	740:744	arg1	fetuin					753:758	fetal bovine fetuin	740:758	fetal bovine fetuin	740:758	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	0	41	theme	fluorophore-conjugated	56:77	arg1	fucose					79:84	fluorophore-conjugated fucose	56:84	fluorophore-conjugated fucose	56:84	Detecting substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis.					
32248235	6	42	theme	X	976:976	arg1	structures					978:987	Lewis X and sialyl Lewis X structures	951:987	Lewis X and sialyl Lewis X structures	951:987	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	1	43	from	glycoproteins	213:225	arg1	ends					186:189	the nonreducing ends	170:189	the nonreducing ends of various glycans on glycoproteins	170:225	Like sialylation, fucose usually locates at the nonreducing ends of various glycans on glycoproteins and constitutes important glycan epitopes.					
32248235	6	44	theme	Lewis	970:974	arg1	X					976:976	sialyl Lewis X	963:976	sialyl Lewis X	963:976	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	4	45	theme	glycans	712:718	arg1	detection					685:693	the detection	681:693	the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies	681:823	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	1	46	theme	glycan	253:258	arg1	epitopes					260:267	important glycan epitopes	243:267	important glycan epitopes	243:267	Like sialylation, fucose usually locates at the nonreducing ends of various glycans on glycoproteins and constitutes important glycan epitopes.					
32248235	4	47	theme	therapeutic	802:812	arg1	antibodies					814:823	therapeutic antibodies	802:823	therapeutic antibodies	802:823	Specifically, we describe the detection of the substrate glycans of these enzymes on fetal bovine fetuin, recombinant H1N1 viral neuraminidase and therapeutic antibodies.					
32248235	2	48	dep	conditions	428:437	arg1	response					399:406	response	399:406	response	399:406	Detecting the substrate glycans of fucosyltransferases is important for understanding how these glycan epitopes are regulated in response to different growth conditions and external stimuli.					
32248235	1	49	from	glycans	202:208	arg1	glycoproteins					213:225	glycoproteins	213:225	glycoproteins	213:225	Like sialylation, fucose usually locates at the nonreducing ends of various glycans on glycoproteins and constitutes important glycan epitopes.					
32248235	3	50	theme	enzymatic	559:567	arg1	incorporation					569:581	enzymatic incorporation	559:581	enzymatic incorporation of fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9	559:652	Here we report the detection of these glycans on glycoproteins as well as in their free forms via enzymatic incorporation of fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9.					
32248235	0	51	theme	methods	90:96	arg1	glycans					20:26	substrate glycans	10:26	substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis	10:123	Detecting substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis.					
32248235	6	52	theme	enzymes	1156:1162	arg1	specificities					1107:1119	the substrate specificities	1093:1119	the substrate specificities	1093:1119	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	6	52	theme	enzymes	1156:1162	arg1	features					1138:1145	some kinetic features	1125:1145	some kinetic features	1125:1145	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	2	53	theme	substrate	284:292	arg1	glycans					294:300	the substrate glycans	280:300	the substrate glycans of fucosyltransferases	280:323	Detecting the substrate glycans of fucosyltransferases is important for understanding how these glycan epitopes are regulated in response to different growth conditions and external stimuli.					
32248235	3	54	from	detection	480:488	arg1	forms					549:553	their free forms	538:553	their free forms	538:553	Here we report the detection of these glycans on glycoproteins as well as in their free forms via enzymatic incorporation of fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9.					
32248235	3	54	from	detection	480:488	arg1	glycoproteins					510:522	glycoproteins	510:522	glycoproteins	510:522	Here we report the detection of these glycans on glycoproteins as well as in their free forms via enzymatic incorporation of fluorophore-conjugated fucose using FUT2, FUT6, FUT7, FUT8 and FUT9.					
32248235	2	55	theme	growth	421:426	arg1	conditions					428:437	different growth conditions	411:437	different growth conditions	411:437	Detecting the substrate glycans of fucosyltransferases is important for understanding how these glycan epitopes are regulated in response to different growth conditions and external stimuli.					
32248235	2	56	theme	external	443:450	arg1	stimuli					452:458	external stimuli	443:458	external stimuli	443:458	Detecting the substrate glycans of fucosyltransferases is important for understanding how these glycan epitopes are regulated in response to different growth conditions and external stimuli.					
32248235	7	57	theme	Lewis	1288:1292	arg1	X					1294:1294	sialyl Lewis X	1281:1294	sialyl Lewis X	1281:1294	Our results support the notion that fucosyltransferases are key targets for regulating the synthesis of Lewis X and sialyl Lewis X structures.					
32248235	6	58	theme	sialyl	963:968	arg1	X					976:976	sialyl Lewis X	963:976	sialyl Lewis X	963:976	By establishing a series of precursors for the synthesis of Lewis X and sialyl Lewis X structures, we not only provide convenient electrophoresis methods for studying glycosylation but also demonstrate the substrate specificities and some kinetic features of these enzymes.					
32248235	2	59	theme	different	411:419	arg1	conditions					428:437	different growth conditions	411:437	different growth conditions	411:437	Detecting the substrate glycans of fucosyltransferases is important for understanding how these glycan epitopes are regulated in response to different growth conditions and external stimuli.					
32248235	7	60	theme	X	1294:1294	arg1	structures					1296:1305	Lewis X and sialyl Lewis X structures	1269:1305	Lewis X and sialyl Lewis X structures	1269:1305	Our results support the notion that fucosyltransferases are key targets for regulating the synthesis of Lewis X and sialyl Lewis X structures.					
32248235	7	61	theme	Lewis	1269:1273	arg1	X					1275:1275	Lewis X	1269:1275	Lewis X	1269:1275	Our results support the notion that fucosyltransferases are key targets for regulating the synthesis of Lewis X and sialyl Lewis X structures.					
33376194	7	0	theme	buffer	1668:1673	arg1	systems					1675:1681	existing buffer systems	1659:1681	existing buffer systems	1659:1681	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	9	1	theme	statistical	2121:2131	arg1	significance					2133:2144	powered statistical significance	2113:2144	powered statistical significance	2113:2144	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	8	2	theme	mass	1830:1833	arg1	capabilities					1835:1846	high-resolution/accurate mass capabilities	1805:1846	high-resolution/accurate mass capabilities	1805:1846	Furthermore, our workflow does not require a mass spectrometer with high-resolution/accurate mass capabilities.					
33376194	10	3	theme	LC-MSn	2151:2156	arg1	approach					2158:2165	The LC-MSn approach we	2147:2168	The LC-MSn approach we	2147:2168	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	6	4	theme	linkage	1429:1435	arg1	information					1437:1447	valuable monosaccharide linkage information	1405:1447	valuable monosaccharide linkage information	1405:1447	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	9	5	theme	evolving	1861:1868	arg1	appreciation					1870:1881	The rapidly evolving appreciation	1849:1881	The rapidly evolving appreciation of the biological significance of glycans for human health and disease	1849:1952	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	10	6	with	autosampling	2273:2284	arg1	enhancements					2313:2324	associated throughput enhancements	2291:2324	associated throughput enhancements	2291:2324	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	10	7	theme	isomeric	2194:2201	arg1	separations					2203:2213	glycan isomeric separations	2187:2213	glycan isomeric separations	2187:2213	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	6	8	theme	valuable	1405:1412	arg1	information					1437:1447	valuable monosaccharide linkage information	1405:1447	valuable monosaccharide linkage information	1405:1447	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	3	9	theme	orthogonal	621:630	arg1	degradation					632:642	secondary orthogonal degradation	611:642	secondary orthogonal degradation	611:642	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	7	10	theme	minimal	1636:1642	arg1	deviation					1644:1652	minimal deviation	1636:1652	minimal deviation from existing buffer systems, chromatography media, and instrument configurations	1636:1734	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	6	11	theme	mobile	1292:1297	arg1	phases					1299:1304	mobile phases	1292:1304	mobile phases	1292:1304	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	4	12	theme	permethylated	747:759	arg1	derivatives					761:771	their permethylated derivatives	741:771	their permethylated derivatives	741:771	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	1	13	theme	complex	186:192	arg1	analytes					194:201	complex analytes	186:201	complex analytes	186:201	HPLC has been employed for decades to enhance detection sensitivity and quantification of complex analytes within biological mixtures.					
33376194	9	14	theme	biological	1890:1899	arg1	significance					1901:1912	the biological significance	1886:1912	the biological significance of glycans for human health and disease	1886:1952	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	10	15	theme	structural	2226:2235	arg1	characterization					2237:2252	robust structural characterization	2219:2252	robust structural characterization	2219:2252	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	6	16	theme	cross-ring	1370:1379	arg1	fragmentation					1381:1393	cross-ring fragmentation	1370:1393	cross-ring fragmentation to obtain valuable monosaccharide linkage information	1370:1447	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	3	17	theme	strategies	644:653	arg1	availability					574:585	the availability	570:585	the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments	570:688	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	5	18	theme	LC-MS	1021:1025	arg1	analysis					1027:1034	LC-MS analysis	1021:1034	LC-MS analysis of permethylated glycans	1021:1059	Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly.					
33376194	10	19	theme	associated	2291:2300	arg1	enhancements					2313:2324	associated throughput enhancements	2291:2324	associated throughput enhancements	2291:2324	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	7	20	theme	proteomics-based	1494:1509	arg1	workflows					1511:1519	standard proteomics-based workflows	1485:1519	standard proteomics-based workflows	1485:1519	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	0	21	theme	Reversed	65:72	arg1	NanoLC-NSI-MSn					80:93	Reversed Phase NanoLC-NSI-MSn	65:93	Reversed Phase NanoLC-NSI-MSn	65:93	Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MSn.					
33376194	4	22	theme	multidimensional	795:810	arg1	fragmentation					816:828	in-line multidimensional ion fragmentation	787:828	in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards	787:889	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	4	22	theme	multidimensional	795:810	arg1	MSn					831:833	MSn	831:833	MSn	831:833	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	9	23	theme	methods	2001:2007	arg1	implementation					1967:1980	the implementation	1963:1980	the implementation of high-throughput methods	1963:2007	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	4	24	theme	independent	866:876	arg1	features					857:864	structural features	846:864	structural features independent of standards	846:889	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	4	25	from	samples	970:976	arg1	depth					924:928	the depth	920:928	the depth of knowledge obtainable from biological samples	920:976	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	8	26	with	spectrometer	1787:1798	arg1	capabilities					1835:1846	high-resolution/accurate mass capabilities	1805:1846	high-resolution/accurate mass capabilities	1805:1846	Furthermore, our workflow does not require a mass spectrometer with high-resolution/accurate mass capabilities.					
33376194	4	27	theme	standards	881:889	arg1	independent					866:876	independent	866:876	independent	866:876	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	5	28	theme	mobile	1095:1100	arg1	optimization					1108:1119	mobile phase optimization	1095:1119	mobile phase optimization	1095:1119	Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly.					
33376194	7	29	theme	standard	1485:1492	arg1	workflows					1511:1519	standard proteomics-based workflows	1485:1519	standard proteomics-based workflows	1485:1519	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	3	30	theme	glycan	590:595	arg1	standards					597:605	glycan standards	590:605	glycan standards	590:605	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	7	31	with	laboratories	1564:1575	arg1	expertise					1582:1590	expertise	1582:1590	expertise in proteomics	1582:1604	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	5	32	theme	glycans	1053:1059	arg1	analysis					1027:1034	LC-MS analysis	1021:1034	LC-MS analysis of permethylated glycans	1021:1059	Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly.					
33376194	10	33	dep	report	2170:2175	arg1	generates					2177:2185	generates	2177:2185	generates glycan isomeric separations and robust structural characterization	2177:2252	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	10	33	dep	report	2170:2175	arg1	amenable					2261:2268	amenable	2261:2268	amenable	2261:2268	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	4	34	theme	structural	846:855	arg1	features					857:864	structural features	846:864	structural features independent of standards	846:889	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	3	35	theme	glycoprotein	516:527	arg1	glycosylation					544:556	glycoprotein and glycolipid glycosylation	516:556	glycosylation	544:556	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	7	36	theme	instrument	1710:1719	arg1	configurations					1721:1734	instrument configurations	1710:1734	instrument configurations	1710:1734	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	9	37	theme	sample	2057:2062	arg1	sets					2064:2067	sample sets	2057:2067	sample sets of sufficient size	2057:2086	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	9	37	theme	sample	2057:2062	arg1	size					2083:2086	sufficient size	2072:2086	sufficient size	2072:2086	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	0	38	theme	Permethylated	33:45	arg1	Isomers					54:60	Permethylated Glycan Isomers	33:60	Permethylated Glycan Isomers	33:60	Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MSn.					
33376194	7	39	dep	adapted	1472:1478	arg1	provides					1537:1544	provides	1537:1544	provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations	1537:1734	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	4	40	theme	knowledge	933:941	arg1	obtainable					943:952	knowledge obtainable	933:952	knowledge obtainable	933:952	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	0	41	theme	Isomers	54:60	arg1	Identification					15:28	Identification	15:28	Identification	15:28	Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MSn.					
33376194	0	41	theme	Isomers	54:60	arg1	Separation					0:9	Separation	0:9	Separation	0:9	Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MSn.					
33376194	7	42	theme	chromatography	1684:1697	arg1	media					1699:1703	chromatography media	1684:1703	chromatography media	1684:1703	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	9	43	theme	size	2083:2086	arg1	sets					2064:2067	sample sets	2057:2067	sample sets of sufficient size	2057:2086	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	9	43	theme	size	2083:2086	arg1	size					2083:2086	sufficient size	2072:2086	sufficient size	2072:2086	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	10	44	dep	approach	2158:2165	arg1	we					2167:2168	we	2167:2168	we	2167:2168	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	5	45	theme	method	1130:1135	arg1	development					1137:1147	MSn method development	1126:1147	MSn method development	1126:1147	Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly.					
33376194	3	46	theme	glycolipid	533:542	arg1	glycosylation					544:556	glycoprotein and glycolipid glycosylation	516:556	glycosylation	544:556	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	7	47	with	data	1626:1629	arg1	deviation					1644:1652	minimal deviation	1636:1652	minimal deviation from existing buffer systems, chromatography media, and instrument configurations	1636:1734	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	6	48	theme	isomeric	1232:1239	arg1	structures					1257:1266	isomeric N- and O-glycan structures	1232:1266	isomeric N- and O-glycan structures	1232:1266	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	4	49	theme	robust	693:698	arg1	method					700:705	A robust method	691:705	A robust method	691:705	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	2	50	theme	detection	409:417	arg1	methods					419:425	various detection methods	401:425	various detection methods	401:425	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
33376194	7	51	theme	existing	1659:1666	arg1	systems					1675:1681	existing buffer systems	1659:1681	existing buffer systems	1659:1681	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	9	52	theme	powered	2113:2119	arg1	significance					2133:2144	powered statistical significance	2113:2144	powered statistical significance	2113:2144	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	7	53	from	media	1699:1703	arg1	deviation					1644:1652	minimal deviation	1636:1652	minimal deviation from existing buffer systems, chromatography media, and instrument configurations	1636:1734	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	8	54	theme	high-resolution/accurate	1805:1828	arg1	capabilities					1835:1846	high-resolution/accurate mass capabilities	1805:1846	high-resolution/accurate mass capabilities	1805:1846	Furthermore, our workflow does not require a mass spectrometer with high-resolution/accurate mass capabilities.					
33376194	6	55	theme	monosaccharide	1414:1427	arg1	information					1437:1447	valuable monosaccharide linkage information	1405:1447	valuable monosaccharide linkage information	1405:1447	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	3	56	theme	secondary	611:619	arg1	degradation					632:642	secondary orthogonal degradation	611:642	secondary orthogonal degradation	611:642	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	10	57	theme	glycan	2187:2192	arg1	separations					2203:2213	glycan isomeric separations	2187:2213	glycan isomeric separations	2187:2213	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	3	58	theme	standards	597:605	arg1	strategies					644:653	glycan standards and secondary orthogonal degradation strategies	590:653	glycan standards and secondary orthogonal degradation strategies	590:653	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	7	59	from	configurations	1721:1734	arg1	deviation					1644:1652	minimal deviation	1636:1652	minimal deviation from existing buffer systems, chromatography media, and instrument configurations	1636:1734	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	7	60	theme	glycomic	1617:1624	arg1	data					1626:1629	glycomic data	1617:1629	glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations	1617:1734	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	4	61	theme	obtainable	943:952	arg1	depth					924:928	the depth	920:928	the depth of knowledge obtainable from biological samples	920:976	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	2	62	gly	glycoproteins	275:287	arg1	glycoproteins					275:287	glycoproteins	275:287	glycoproteins	275:287	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
33376194	1	63	theme	analytes	194:201	arg1	sensitivity					152:162	detection sensitivity	142:162	detection sensitivity	142:162	HPLC has been employed for decades to enhance detection sensitivity and quantification of complex analytes within biological mixtures.					
33376194	1	63	theme	analytes	194:201	arg1	quantification					168:181	quantification	168:181	quantification	168:181	HPLC has been employed for decades to enhance detection sensitivity and quantification of complex analytes within biological mixtures.					
33376194	4	64	theme	biological	959:968	arg1	samples					970:976	biological samples	959:976	biological samples	959:976	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	10	65	theme	robust	2219:2224	arg1	characterization					2237:2252	robust structural characterization	2219:2252	robust structural characterization	2219:2252	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	3	66	theme	degradation	632:642	arg1	strategies					644:653	glycan standards and secondary orthogonal degradation strategies	590:653	glycan standards and secondary orthogonal degradation strategies	590:653	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	1	67	theme	biological	210:219	arg1	mixtures					221:228	biological mixtures	210:228	biological mixtures	210:228	HPLC has been employed for decades to enhance detection sensitivity and quantification of complex analytes within biological mixtures.					
33376194	9	68	theme	glycans	1917:1923	arg1	significance					1901:1912	the biological significance	1886:1912	the biological significance of glycans for human health and disease	1886:1952	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	6	69	theme	structures	1257:1266	arg1	MSn					1225:1227	MSn	1225:1227	MSn	1225:1227	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	6	69	theme	structures	1257:1266	arg1	separation					1210:1219	baseline separation	1201:1219	baseline separation	1201:1219	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	9	70	theme	significance	1901:1912	arg1	appreciation					1870:1881	The rapidly evolving appreciation	1849:1881	The rapidly evolving appreciation of the biological significance of glycans for human health and disease	1849:1952	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	9	71	theme	human	1929:1933	arg1	health					1935:1940	human health	1929:1940	human health	1929:1940	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	4	72	theme	glycans	730:736	arg1	separation					716:725	HPLC separation	711:725	HPLC separation of glycans as their permethylated derivatives	711:771	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	3	73	theme	structural	667:676	arg1	assignments					678:688	structural assignments	667:688	structural assignments	667:688	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	6	74	theme	adduct	1333:1338	arg1	heterogeneity					1340:1352	adduct heterogeneity	1333:1352	adduct heterogeneity	1333:1352	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	4	75	theme	ion	812:814	arg1	fragmentation					816:828	in-line multidimensional ion fragmentation	787:828	in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards	787:889	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	4	75	theme	ion	812:814	arg1	MSn					831:833	MSn	831:833	MSn	831:833	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	5	76	theme	structural	1160:1169	arg1	isomers					1171:1177	structural isomers	1160:1177	structural isomers	1160:1177	Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly.					
33376194	2	77	theme	underivatized	332:344	arg1	glycans					253:259	glycans	253:259	glycans released from glycoproteins and glycolipids	253:303	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
33376194	2	77	theme	underivatized	332:344	arg1	derivatives					370:380	underivatized or fluorescently tagged derivatives	332:380	underivatized or fluorescently tagged derivatives	332:380	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
33376194	0	78	theme	Phase	74:78	arg1	NanoLC-NSI-MSn					80:93	Reversed Phase NanoLC-NSI-MSn	65:93	Reversed Phase NanoLC-NSI-MSn	65:93	Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MSn.					
33376194	1	79	theme	detection	142:150	arg1	sensitivity					152:162	detection sensitivity	142:162	detection sensitivity	142:162	HPLC has been employed for decades to enhance detection sensitivity and quantification of complex analytes within biological mixtures.					
33376194	7	80	from	expertise	1582:1590	arg1	proteomics					1595:1604	proteomics	1595:1604	proteomics	1595:1604	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	2	81	attach	released	261:268	arg1	glycoproteins					275:287	glycoproteins	275:287	glycoproteins	275:287	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
33376194	2	81	attach	released	261:268	arg1	glycolipids					293:303	glycolipids	293:303	glycolipids	293:303	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
33376194	2	81	attach	released	261:268	arg2	glycans					253:259	glycans	253:259	glycans released from glycoproteins and glycolipids	253:303	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
33376194	2	81	attach	released	261:268	arg2	derivatives					370:380	underivatized or fluorescently tagged derivatives	332:380	underivatized or fluorescently tagged derivatives	332:380	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
33376194	5	82	theme	optimized	998:1006	arg1	workflow					1008:1015	an optimized workflow	995:1015	an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly	995:1188	Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly.					
33376194	9	83	theme	high-throughput	1985:1999	arg1	methods					2001:2007	high-throughput methods	1985:2007	high-throughput methods	1985:2007	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	4	84	theme	in-line	787:793	arg1	fragmentation					816:828	in-line multidimensional ion fragmentation	787:828	in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards	787:889	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	4	84	theme	in-line	787:793	arg1	MSn					831:833	MSn	831:833	MSn	831:833	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	5	85	theme	sample	1075:1080	arg1	preparation					1082:1092	sample preparation	1075:1092	sample preparation	1075:1092	Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly.					
33376194	2	86	theme	tagged	363:368	arg1	glycans					253:259	glycans	253:259	glycans released from glycoproteins and glycolipids	253:303	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
33376194	2	86	theme	tagged	363:368	arg1	derivatives					370:380	underivatized or fluorescently tagged derivatives	332:380	underivatized or fluorescently tagged derivatives	332:380	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
33376194	6	87	theme	baseline	1201:1208	arg1	separation					1210:1219	baseline separation	1201:1219	baseline separation	1201:1219	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	10	88	theme	throughput	2302:2311	arg1	enhancements					2313:2324	associated throughput enhancements	2291:2324	associated throughput enhancements	2291:2324	The LC-MSn approach we report generates glycan isomeric separations and robust structural characterization and is amenable to autosampling with associated throughput enhancements.					
33376194	3	89	theme	structural	492:501	arg1	diversity					503:511	the structural diversity	488:511	the structural diversity of glycoprotein and glycolipid glycosylation	488:556	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	5	90	theme	permethylated	1039:1051	arg1	glycans					1053:1059	permethylated glycans	1039:1059	permethylated glycans	1039:1059	Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly.					
33376194	0	91	theme	Glycan	47:52	arg1	Isomers					54:60	Permethylated Glycan Isomers	33:60	Permethylated Glycan Isomers	33:60	Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MSn.					
33376194	5	92	theme	MSn	1126:1128	arg1	development					1137:1147	MSn method development	1126:1147	MSn method development	1126:1147	Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly.					
33376194	3	93	gly	glycoprotein	516:527	arg1	glycoprotein					516:527	glycoprotein and glycolipid glycosylation	516:556	glycoprotein	516:527	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	6	94	theme	O-glycan	1248:1255	arg1	structures					1257:1266	isomeric N- and O-glycan structures	1232:1266	isomeric N- and O-glycan structures	1232:1266	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	9	95	theme	sufficient	2072:2081	arg1	size					2083:2086	sufficient size	2072:2086	sufficient size	2072:2086	The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance.					
33376194	5	96	theme	phase	1102:1106	arg1	optimization					1108:1119	mobile phase optimization	1095:1119	mobile phase optimization	1095:1119	Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly.					
33376194	6	97	theme	N-	1241:1242	arg1	structures					1257:1266	isomeric N- and O-glycan structures	1232:1266	isomeric N- and O-glycan structures	1232:1266	We report baseline separation and MSn of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information.					
33376194	8	98	theme	mass	1782:1785	arg1	spectrometer					1787:1798	a mass spectrometer	1780:1798	a mass spectrometer with high-resolution/accurate mass capabilities	1780:1846	Furthermore, our workflow does not require a mass spectrometer with high-resolution/accurate mass capabilities.					
33376194	3	99	theme	glycosylation	544:556	arg1	diversity					503:511	the structural diversity	488:511	the structural diversity of glycoprotein and glycolipid glycosylation	488:556	These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments.					
33376194	7	100	from	systems	1675:1681	arg1	deviation					1644:1652	minimal deviation	1636:1652	minimal deviation from existing buffer systems, chromatography media, and instrument configurations	1636:1734	Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations.					
33376194	4	101	theme	HPLC	711:714	arg1	separation					716:725	HPLC separation	711:725	HPLC separation of glycans as their permethylated derivatives	711:771	A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSn) to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples.					
33376194	2	102	theme	various	401:407	arg1	methods					419:425	various detection methods	401:425	various detection methods	401:425	Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods.					
34411478	1	0	theme	glucosylation	65:77	arg1	strategy					79:86	a glucosylation strategy	63:86	a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors	63:276	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	2	1	theme	α-glucosylation	320:334	arg1	strategy					336:343	the α-glucosylation strategy	316:343	the α-glucosylation strategy	316:343	The versatility and effectiveness of the α-glucosylation strategy were demonstrated successfully with various acceptors, including complex alcohols.					
34411478	3	2	theme	modular	478:484	arg1	synthesis					486:494	the modular synthesis	474:494	the modular synthesis of various α-glucans with both linear and branched backbone structures	474:565	This approach demonstrates the feasibility of the modular synthesis of various α-glucans with both linear and branched backbone structures.					
34411478	1	3	theme	stereoselective	150:164	arg1	construction					166:177	the highly stereoselective construction	139:177	the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors	139:276	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	3	4	theme	various	499:505	arg1	α-glucans					507:515	various α-glucans	499:515	various α-glucans with both linear and branched backbone structures	499:565	This approach demonstrates the feasibility of the modular synthesis of various α-glucans with both linear and branched backbone structures.					
34411478	0	5	theme	Stereocontrolled	14:29	arg1	α-Glucosylation					31:45	ZnI2-Directed Stereocontrolled α-Glucosylation	0:45	ZnI2-Directed Stereocontrolled α-Glucosylation.	0:46	ZnI2-Directed Stereocontrolled α-Glucosylation.					
34411478	2	6	dep	versatility	283:293	arg1	The					279:281	The	279:281	The	279:281	The versatility and effectiveness of the α-glucosylation strategy were demonstrated successfully with various acceptors, including complex alcohols.					
34411478	0	7	theme	ZnI2-Directed	0:12	arg1	α-Glucosylation					31:45	ZnI2-Directed Stereocontrolled α-Glucosylation	0:45	ZnI2-Directed Stereocontrolled α-Glucosylation.	0:46	ZnI2-Directed Stereocontrolled α-Glucosylation.					
34411478	3	8	theme	synthesis	486:494	arg1	feasibility					459:469	the feasibility	455:469	the feasibility of the modular synthesis of various α-glucans with both linear and branched backbone structures	455:565	This approach demonstrates the feasibility of the modular synthesis of various α-glucans with both linear and branched backbone structures.					
34411478	3	9	with	α-glucans	507:515	arg1	structures					556:565	both linear and branched backbone structures	522:565	both linear and branched backbone structures	522:565	This approach demonstrates the feasibility of the modular synthesis of various α-glucans with both linear and branched backbone structures.					
34411478	2	10	theme	strategy	336:343	arg1	effectiveness					299:311	effectiveness	299:311	effectiveness	299:311	The versatility and effectiveness of the α-glucosylation strategy were demonstrated successfully with various acceptors, including complex alcohols.					
34411478	2	10	theme	strategy	336:343	arg1	versatility					283:293	versatility	283:293	versatility	283:293	The versatility and effectiveness of the α-glucosylation strategy were demonstrated successfully with various acceptors, including complex alcohols.					
34411478	1	11	theme	linkages	203:210	arg1	construction					166:177	the highly stereoselective construction	139:177	the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors	139:276	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	1	12	theme	cheap	108:112	arg1	acid					129:132	a cheap and mild Lewis acid	106:132	a cheap and mild Lewis acid	106:132	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	1	12	theme	cheap	108:112	arg1	ZnI2					100:103	ZnI2	100:103	ZnI2	100:103	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	3	13	theme	linear	527:532	arg1	structures					556:565	both linear and branched backbone structures	522:565	both linear and branched backbone structures	522:565	This approach demonstrates the feasibility of the modular synthesis of various α-glucans with both linear and branched backbone structures.					
34411478	2	14	theme	various	381:387	arg1	acceptors					389:397	various acceptors	381:397	various acceptors	381:397	The versatility and effectiveness of the α-glucosylation strategy were demonstrated successfully with various acceptors, including complex alcohols.					
34411478	2	14	theme	various	381:387	arg1	alcohols					418:425	complex alcohols	410:425	complex alcohols	410:425	The versatility and effectiveness of the α-glucosylation strategy were demonstrated successfully with various acceptors, including complex alcohols.					
34411478	1	15	theme	1,2-cis-O-glycosidic	182:201	arg1	linkages					203:210	1,2-cis-O-glycosidic linkages	182:210	1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors	182:276	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	3	16	theme	α-glucans	507:515	arg1	synthesis					486:494	the modular synthesis	474:494	the modular synthesis of various α-glucans with both linear and branched backbone structures	474:565	This approach demonstrates the feasibility of the modular synthesis of various α-glucans with both linear and branched backbone structures.					
34411478	1	17	theme	accessible	225:234	arg1	donors					271:276	easily accessible and common 4,6-O-tethered glucosyl donors	218:276	easily accessible and common 4,6-O-tethered glucosyl donors	218:276	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	3	18	theme	backbone	547:554	arg1	structures					556:565	both linear and branched backbone structures	522:565	both linear and branched backbone structures	522:565	This approach demonstrates the feasibility of the modular synthesis of various α-glucans with both linear and branched backbone structures.					
34411478	1	19	theme	mild	118:121	arg1	acid					129:132	a cheap and mild Lewis acid	106:132	a cheap and mild Lewis acid	106:132	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	1	19	theme	mild	118:121	arg1	ZnI2					100:103	ZnI2	100:103	ZnI2	100:103	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	1	20	theme	Lewis	123:127	arg1	acid					129:132	a cheap and mild Lewis acid	106:132	a cheap and mild Lewis acid	106:132	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	1	20	theme	Lewis	123:127	arg1	ZnI2					100:103	ZnI2	100:103	ZnI2	100:103	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	3	21	theme	branched	538:545	arg1	structures					556:565	both linear and branched backbone structures	522:565	both linear and branched backbone structures	522:565	This approach demonstrates the feasibility of the modular synthesis of various α-glucans with both linear and branched backbone structures.					
34411478	1	22	theme	4,6-O-tethered	247:260	arg1	donors					271:276	easily accessible and common 4,6-O-tethered glucosyl donors	218:276	easily accessible and common 4,6-O-tethered glucosyl donors	218:276	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
34411478	2	23	theme	complex	410:416	arg1	alcohols					418:425	complex alcohols	410:425	complex alcohols	410:425	The versatility and effectiveness of the α-glucosylation strategy were demonstrated successfully with various acceptors, including complex alcohols.					
34411478	1	24	theme	glucosyl	262:269	arg1	donors					271:276	easily accessible and common 4,6-O-tethered glucosyl donors	218:276	easily accessible and common 4,6-O-tethered glucosyl donors	218:276	Here we report a glucosylation strategy mediated by ZnI2, a cheap and mild Lewis acid, for the highly stereoselective construction of 1,2-cis-O-glycosidic linkages using easily accessible and common 4,6-O-tethered glucosyl donors.					
32104808	6	0	theme	glycoproteins	1127:1139	arg1	mixture					1106:1112	a simple mixture	1097:1112	a simple mixture of mammalian glycoproteins	1097:1139	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	6	0	theme	glycoproteins	1127:1139	arg1	wall					1074:1077	a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins	1042:1139	wall	1074:1077	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	7	1	theme	DIALib	1160:1165	arg1	libraries					1167:1175	DIALib libraries	1160:1175	DIALib libraries consisting only of glycan oxonium ions	1160:1214	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	1	2	theme	peptides	247:254	arg1	measurement					217:227	unbiased measurement	208:227	unbiased measurement of all detectable peptides from complex proteomes	208:277	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	4	3	theme	peptide	734:740	arg1	construction					718:729	the construction	714:729	the construction of peptide and glycopeptide Data Independent Acquisition ion Libraries	714:800	Here, we present DIALib, software to automate the construction of peptide and glycopeptide Data Independent Acquisition ion Libraries.					
32104808	1	4	theme	complex	261:267	arg1	proteomes					269:277	complex proteomes	261:277	complex proteomes	261:277	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	6	5	from	wall	1074:1077	arg1	data					1032:1035	proof-of-principle data	1013:1035	proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins	1013:1139	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	1	6	theme	Mass	169:172	arg1	Spectrometry					174:185	Data Independent Acquisition (DIA) Mass Spectrometry	134:185	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows	134:200	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	1	6	theme	Mass	169:172	arg1	MS					188:189	MS	188:189	MS	188:189	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	3	7	theme	peptide	555:561	arg1	identification					563:576	confident peptide identification	545:576	confident peptide identification	545:576	However, DDA libraries derived from empirical data rely on confident peptide identification, which can be challenging for peptides carrying complex post-translational modifications.					
32104808	5	8	gly	glycoproteins	930:942	arg1	glycoproteins					930:942	mammalian glycoproteins	920:942	mammalian glycoproteins	920:942	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	5	9	theme	theoretical	823:833	arg1	libraries					839:847	DIALib theoretical ion libraries	816:847	DIALib theoretical ion libraries	816:847	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	2	10	from	workflows	475:483	arg1	data					429:432	peptide identification data	406:432	peptide identification data from Data Dependent Acquisition (DDA) MS workflows	406:483	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32104808	0	11	gly	glycopeptides	119:131	arg2	glycopeptides					119:131	glycopeptides	119:131	glycopeptides	119:131	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	1	12	from	measurement	217:227	arg1	proteomes					269:277	complex proteomes	261:277	complex proteomes	261:277	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	5	13	theme	prior	952:956	arg1	knowledge					958:966	prior knowledge	952:966	prior knowledge of the glycan structures present	952:999	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	6	14	theme	complex	1055:1061	arg1	wall					1074:1077	a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins	1042:1139	wall	1074:1077	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	7	15	theme	glycosylation	1270:1282	arg1	profile					1284:1290	a global compositional glycosylation profile	1247:1290	a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes	1247:1337	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	5	16	theme	ion	835:837	arg1	libraries					839:847	DIALib theoretical ion libraries	816:847	DIALib theoretical ion libraries	816:847	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	1	17	theme	Acquisition	151:161	arg1	Spectrometry					174:185	Data Independent Acquisition (DIA) Mass Spectrometry	134:185	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows	134:200	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	1	17	theme	Acquisition	151:161	arg1	MS					188:189	MS	188:189	MS	188:189	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	2	18	theme	MS	472:473	arg1	workflows					475:483	Data Dependent Acquisition (DDA) MS workflows	439:483	Data Dependent Acquisition (DDA) MS workflows	439:483	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32104808	0	19	theme	acquisition	64:74	arg1	analysis					94:101	data independent acquisition mass spectrometry analysis	47:101	data independent acquisition mass spectrometry analysis of peptides and glycopeptides	47:131	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	5	20	from	yeast	910:914	arg1	O-glycopeptides					889:903	O-glycopeptides	889:903	O-glycopeptides	889:903	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	5	20	from	yeast	910:914	arg1	N-					882:883	diverse N-	874:883	diverse N-	874:883	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	6	21	dep	wall	1074:1077	arg1	glycoproteome					1079:1091	glycoproteome	1079:1091	glycoproteome	1079:1091	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	0	22	theme	spectrometry	81:92	arg1	analysis					94:101	data independent acquisition mass spectrometry analysis	47:101	data independent acquisition mass spectrometry analysis of peptides and glycopeptides	47:131	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	5	23	theme	present	993:999	arg1	structures					982:991	the glycan structures	971:991	the glycan structures present	971:999	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	1	24	theme	Data	134:137	arg1	Acquisition					151:161	Data Independent Acquisition	134:161	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows	134:200	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	1	24	theme	Data	134:137	arg1	DIA					164:166	DIA	164:166	DIA	164:166	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	4	25	theme	ion	788:790	arg1	Libraries					792:800	Data Independent Acquisition ion Libraries	759:800	Data Independent Acquisition ion Libraries	759:800	Here, we present DIALib, software to automate the construction of peptide and glycopeptide Data Independent Acquisition ion Libraries.					
32104808	4	26	gly	glycopeptide	746:757	arg2	glycopeptide					746:757	glycopeptide	746:757	glycopeptide	746:757	Here, we present DIALib, software to automate the construction of peptide and glycopeptide Data Independent Acquisition ion Libraries.					
32104808	2	27	theme	Data	439:442	arg1	workflows					475:483	Data Dependent Acquisition (DDA) MS workflows	439:483	Data Dependent Acquisition (DDA) MS workflows	439:483	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32104808	0	28	theme	independent	52:62	arg1	analysis					94:101	data independent acquisition mass spectrometry analysis	47:101	data independent acquisition mass spectrometry analysis of peptides and glycopeptides	47:131	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	7	29	gly	glycosylation	1270:1282	arg1	"					1319:1319	the detectable "oxoniome"	1295:1319	the detectable "oxoniome" of glycoproteomes	1295:1337	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	5	30	theme	DIALib	816:821	arg1	libraries					839:847	DIALib theoretical ion libraries	816:847	DIALib theoretical ion libraries	816:847	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	4	31	theme	glycopeptide	746:757	arg1	construction					718:729	the construction	714:729	the construction of peptide and glycopeptide Data Independent Acquisition ion Libraries	714:800	Here, we present DIALib, software to automate the construction of peptide and glycopeptide Data Independent Acquisition ion Libraries.					
32104808	2	32	theme	peptide	406:412	arg1	data					429:432	peptide identification data	406:432	peptide identification data from Data Dependent Acquisition (DDA) MS workflows	406:483	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32104808	7	33	theme	oxonium	1203:1209	arg1	ions					1211:1214	glycan oxonium ions	1196:1214	glycan oxonium ions	1196:1214	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	5	34	theme	glycan	975:980	arg1	structures					982:991	the glycan structures	971:991	the glycan structures present	971:999	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	2	35	theme	DDA	467:469	arg1	workflows					475:483	Data Dependent Acquisition (DDA) MS workflows	439:483	Data Dependent Acquisition (DDA) MS workflows	439:483	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32104808	0	36	theme	data	47:50	arg1	analysis					94:101	data independent acquisition mass spectrometry analysis	47:101	data independent acquisition mass spectrometry analysis of peptides and glycopeptides	47:131	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	0	37	theme	ion	21:23	arg1	generator					33:41	an automated ion library generator	8:41	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.	0:132	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	1	38	theme	ion	292:294	arg1	libraries					296:304	ion libraries	292:304	ion libraries for interrogation of peptides of interest	292:346	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	6	39	theme	cell	1069:1072	arg1	wall					1074:1077	a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins	1042:1139	wall	1074:1077	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	2	40	theme	Acquisition	454:464	arg1	workflows					475:483	Data Dependent Acquisition (DDA) MS workflows	439:483	Data Dependent Acquisition (DDA) MS workflows	439:483	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32104808	3	41	theme	complex	626:632	arg1	modifications					653:665	complex post-translational modifications	626:665	complex post-translational modifications	626:665	However, DDA libraries derived from empirical data rely on confident peptide identification, which can be challenging for peptides carrying complex post-translational modifications.					
32104808	1	42	theme	unbiased	208:215	arg1	measurement					217:227	unbiased measurement	208:227	unbiased measurement of all detectable peptides from complex proteomes	208:277	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	7	43	theme	oxoniome	1311:1318	arg1	"					1319:1319	the detectable "oxoniome"	1295:1319	the detectable "oxoniome" of glycoproteomes	1295:1337	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	3	44	theme	DDA	495:497	arg1	libraries					499:507	DDA libraries	495:507	DDA libraries derived from empirical data	495:535	However, DDA libraries derived from empirical data rely on confident peptide identification, which can be challenging for peptides carrying complex post-translational modifications.					
32104808	5	45	theme	diverse	874:880	arg1	N-					882:883	diverse N-	874:883	diverse N-	874:883	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	0	46	dep	DIALib	0:5	arg1	generator					33:41	an automated ion library generator	8:41	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.	0:132	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	7	47	theme	detectable	1299:1308	arg1	"					1319:1319	the detectable "oxoniome"	1295:1319	the detectable "oxoniome" of glycoproteomes	1295:1337	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	1	48	theme	detectable	236:245	arg1	peptides					247:254	all detectable peptides	232:254	all detectable peptides from complex proteomes	232:277	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	6	49	theme	mammalian	1117:1125	arg1	glycoproteins					1127:1139	mammalian glycoproteins	1117:1139	mammalian glycoproteins	1117:1139	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	3	50	theme	empirical	522:530	arg1	data					532:535	empirical data	522:535	empirical data	522:535	However, DDA libraries derived from empirical data rely on confident peptide identification, which can be challenging for peptides carrying complex post-translational modifications.					
32104808	6	51	from	mixture	1106:1112	arg1	data					1032:1035	proof-of-principle data	1013:1035	proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins	1013:1139	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	7	52	theme	global	1249:1254	arg1	profile					1284:1290	a global compositional glycosylation profile	1247:1290	a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes	1247:1337	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	1	53	from	proteomes	269:277	arg1	peptides					247:254	all detectable peptides	232:254	all detectable peptides from complex proteomes	232:277	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	1	53	from	proteomes	269:277	arg1	measurement					217:227	unbiased measurement	208:227	unbiased measurement of all detectable peptides from complex proteomes	208:277	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	3	54	theme	confident	545:553	arg1	identification					563:576	confident peptide identification	545:576	confident peptide identification	545:576	However, DDA libraries derived from empirical data rely on confident peptide identification, which can be challenging for peptides carrying complex post-translational modifications.					
32104808	1	55	theme	Spectrometry	174:185	arg1	workflows					192:200	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows	134:200	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows	134:200	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	0	56	theme	glycopeptides	119:131	arg1	analysis					94:101	data independent acquisition mass spectrometry analysis	47:101	data independent acquisition mass spectrometry analysis of peptides and glycopeptides	47:131	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	3	57	contain	carrying	617:624	arg2	modifications					653:665	complex post-translational modifications	626:665	complex post-translational modifications	626:665	However, DDA libraries derived from empirical data rely on confident peptide identification, which can be challenging for peptides carrying complex post-translational modifications.					
32104808	3	57	contain	carrying	617:624	arg1	peptides					608:615	peptides	608:615	peptides carrying complex post-translational modifications	608:665	However, DDA libraries derived from empirical data rely on confident peptide identification, which can be challenging for peptides carrying complex post-translational modifications.					
32104808	5	58	theme	mammalian	920:928	arg1	glycoproteins					930:942	mammalian glycoproteins	920:942	mammalian glycoproteins	920:942	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	5	59	gly	O-glycopeptides	889:903	arg1	yeast					910:914	yeast	910:914	yeast	910:914	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	5	59	gly	O-glycopeptides	889:903	arg2	O-glycopeptides					889:903	O-glycopeptides	889:903	O-glycopeptides	889:903	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	5	59	gly	O-glycopeptides	889:903	arg1	glycoproteins					930:942	mammalian glycoproteins	920:942	mammalian glycoproteins	920:942	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	7	60	theme	glycan	1196:1201	arg1	ions					1211:1214	glycan oxonium ions	1196:1214	glycan oxonium ions	1196:1214	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	4	61	dep	peptide	734:740	arg1	Libraries					792:800	Data Independent Acquisition ion Libraries	759:800	Data Independent Acquisition ion Libraries	759:800	Here, we present DIALib, software to automate the construction of peptide and glycopeptide Data Independent Acquisition ion Libraries.					
32104808	6	62	theme	yeast	1063:1067	arg1	wall					1074:1077	a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins	1042:1139	wall	1074:1077	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	4	63	theme	Independent	764:774	arg1	Libraries					792:800	Data Independent Acquisition ion Libraries	759:800	Data Independent Acquisition ion Libraries	759:800	Here, we present DIALib, software to automate the construction of peptide and glycopeptide Data Independent Acquisition ion Libraries.					
32104808	7	64	theme	compositional	1256:1268	arg1	profile					1284:1290	a global compositional glycosylation profile	1247:1290	a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes	1247:1337	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	1	65	theme	Independent	139:149	arg1	Acquisition					151:161	Data Independent Acquisition	134:161	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows	134:200	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	1	65	theme	Independent	139:149	arg1	DIA					164:166	DIA	164:166	DIA	164:166	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	2	66	from	data	429:432	arg1	theoretical					380:390	theoretical	380:390	theoretical	380:390	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32104808	0	67	theme	mass	76:79	arg1	analysis					94:101	data independent acquisition mass spectrometry analysis	47:101	data independent acquisition mass spectrometry analysis of peptides and glycopeptides	47:131	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	1	68	theme	peptides	327:334	arg1	interrogation					310:322	interrogation	310:322	interrogation of peptides of interest	310:346	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	8	69	theme	DIA	1364:1366	arg1	approach					1414:1421	a complementary analytical approach	1387:1421	a complementary analytical approach to DDA glycoproteomics	1387:1444	DIALib will help enable DIA glycoproteomics as a complementary analytical approach to DDA glycoproteomics.					
32104808	8	69	theme	DIA	1364:1366	arg1	glycoproteomics					1368:1382	DIA glycoproteomics	1364:1382	DIA glycoproteomics	1364:1382	DIALib will help enable DIA glycoproteomics as a complementary analytical approach to DDA glycoproteomics.					
32104808	1	70	theme	interest	339:346	arg1	peptides					327:334	peptides	327:334	peptides of interest	327:346	Data Independent Acquisition (DIA) Mass Spectrometry (MS) workflows allow unbiased measurement of all detectable peptides from complex proteomes, but require ion libraries for interrogation of peptides of interest.					
32104808	5	71	theme	structures	982:991	arg1	knowledge					958:966	prior knowledge	952:966	prior knowledge of the glycan structures present	952:999	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	6	72	theme	proof-of-principle	1013:1030	arg1	data					1032:1035	proof-of-principle data	1013:1035	proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins	1013:1139	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	6	73	gly	glycoproteins	1127:1139	arg1	glycoproteins					1127:1139	mammalian glycoproteins	1117:1139	mammalian glycoproteins	1117:1139	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	4	74	theme	Data	759:762	arg1	Libraries					792:800	Data Independent Acquisition ion Libraries	759:800	Data Independent Acquisition ion Libraries	759:800	Here, we present DIALib, software to automate the construction of peptide and glycopeptide Data Independent Acquisition ion Libraries.					
32104808	0	75	theme	peptides	106:113	arg1	analysis					94:101	data independent acquisition mass spectrometry analysis	47:101	data independent acquisition mass spectrometry analysis of peptides and glycopeptides	47:131	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	4	76	theme	Acquisition	776:786	arg1	Libraries					792:800	Data Independent Acquisition ion Libraries	759:800	Data Independent Acquisition ion Libraries	759:800	Here, we present DIALib, software to automate the construction of peptide and glycopeptide Data Independent Acquisition ion Libraries.					
32104808	6	77	theme	simple	1099:1104	arg1	mixture					1106:1112	a simple mixture	1097:1112	a simple mixture of mammalian glycoproteins	1097:1139	We present proof-of-principle data from a moderately complex yeast cell wall glycoproteome and a simple mixture of mammalian glycoproteins.					
32104808	0	78	theme	automated	11:19	arg1	generator					33:41	an automated ion library generator	8:41	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.	0:132	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	5	79	from	glycoproteins	930:942	arg1	O-glycopeptides					889:903	O-glycopeptides	889:903	O-glycopeptides	889:903	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	5	79	from	glycoproteins	930:942	arg1	N-					882:883	diverse N-	874:883	diverse N-	874:883	We show that DIALib theoretical ion libraries can identify and measure diverse N- and O-glycopeptides from yeast and mammalian glycoproteins without prior knowledge of the glycan structures present.					
32104808	2	80	theme	identification	414:427	arg1	data					429:432	peptide identification data	406:432	peptide identification data from Data Dependent Acquisition (DDA) MS workflows	406:483	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32104808	8	81	theme	analytical	1403:1412	arg1	approach					1414:1421	a complementary analytical approach	1387:1421	a complementary analytical approach to DDA glycoproteomics	1387:1444	DIALib will help enable DIA glycoproteomics as a complementary analytical approach to DDA glycoproteomics.					
32104808	8	81	theme	analytical	1403:1412	arg1	glycoproteomics					1368:1382	DIA glycoproteomics	1364:1382	DIA glycoproteomics	1364:1382	DIALib will help enable DIA glycoproteomics as a complementary analytical approach to DDA glycoproteomics.					
32104808	3	82	theme	post-translational	634:651	arg1	modifications					653:665	complex post-translational modifications	626:665	complex post-translational modifications	626:665	However, DDA libraries derived from empirical data rely on confident peptide identification, which can be challenging for peptides carrying complex post-translational modifications.					
32104808	8	83	theme	DDA	1426:1428	arg1	glycoproteomics					1430:1444	DDA glycoproteomics	1426:1444	DDA glycoproteomics	1426:1444	DIALib will help enable DIA glycoproteomics as a complementary analytical approach to DDA glycoproteomics.					
32104808	2	84	theme	ion	359:361	arg1	libraries					363:371	These DIA ion libraries	349:371	These DIA ion libraries	349:371	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32104808	0	85	theme	library	25:31	arg1	generator					33:41	an automated ion library generator	8:41	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.	0:132	DIALib: an automated ion library generator for data independent acquisition mass spectrometry analysis of peptides and glycopeptides.					
32104808	3	86	attach	derived	509:515	arg2	libraries					499:507	DDA libraries	495:507	DDA libraries derived from empirical data	495:535	However, DDA libraries derived from empirical data rely on confident peptide identification, which can be challenging for peptides carrying complex post-translational modifications.					
32104808	3	86	attach	derived	509:515	arg1	data					532:535	empirical data	522:535	empirical data	522:535	However, DDA libraries derived from empirical data rely on confident peptide identification, which can be challenging for peptides carrying complex post-translational modifications.					
32104808	7	87	theme	glycoproteomes	1324:1337	arg1	"					1319:1319	the detectable "oxoniome"	1295:1319	the detectable "oxoniome" of glycoproteomes	1295:1337	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	7	88	theme	"	1319:1319	arg1	profile					1284:1290	a global compositional glycosylation profile	1247:1290	a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes	1247:1337	We also show that DIALib libraries consisting only of glycan oxonium ions can quickly and easily provide a global compositional glycosylation profile of the detectable "oxoniome" of glycoproteomes.					
32104808	2	89	theme	Dependent	444:452	arg1	workflows					475:483	Data Dependent Acquisition (DDA) MS workflows	439:483	Data Dependent Acquisition (DDA) MS workflows	439:483	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32104808	8	90	theme	complementary	1389:1401	arg1	approach					1414:1421	a complementary analytical approach	1387:1421	a complementary analytical approach to DDA glycoproteomics	1387:1444	DIALib will help enable DIA glycoproteomics as a complementary analytical approach to DDA glycoproteomics.					
32104808	8	90	theme	complementary	1389:1401	arg1	glycoproteomics					1368:1382	DIA glycoproteomics	1364:1382	DIA glycoproteomics	1364:1382	DIALib will help enable DIA glycoproteomics as a complementary analytical approach to DDA glycoproteomics.					
32104808	2	91	theme	DIA	355:357	arg1	libraries					363:371	These DIA ion libraries	349:371	These DIA ion libraries	349:371	These DIA ion libraries can be theoretical or built from peptide identification data from Data Dependent Acquisition (DDA) MS workflows.					
32938911	0	0	theme	prolonged	105:113	arg1	circulation					115:125	prolonged circulation	105:125	prolonged circulation in humans	105:135	Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans.					
32938911	2	1	theme	receptor-destroying	488:506	arg1	enzyme					508:513	a receptor-destroying enzyme	486:513	a receptor-destroying enzyme	486:513	Embecovirus particles contain two surface projections called spike (S) and haemagglutinin-esterase (HE), with S mediating receptor binding and membrane fusion, and HE acting as a receptor-destroying enzyme.					
32938911	2	1	theme	receptor-destroying	488:506	arg1	HE					473:474	HE	473:474	HE	473:474	Embecovirus particles contain two surface projections called spike (S) and haemagglutinin-esterase (HE), with S mediating receptor binding and membrane fusion, and HE acting as a receptor-destroying enzyme.					
32938911	5	2	theme	truncated	798:806	arg1	domain					815:820	a drastically truncated lectin domain	784:820	a drastically truncated lectin domain	784:820	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	4	3	theme	3.4 Å	719:723	arg1	resolution					725:734	3.4 Å resolution	719:734	3.4 Å resolution	719:734	Here we present the cryo-EM structure of the ~80 kDa, heavily glycosylated HKU1 HE at 3.4 Å resolution.					
32938911	6	4	theme	lectin	1027:1032	arg1	domain					1034:1039	the now redundant lectin domain	1009:1039	the now redundant lectin domain	1009:1039	Cryo-EM and mass spectrometry analysis reveals a putative glycan shield on the now redundant lectin domain.					
32938911	5	5	theme	lectin	808:813	arg1	domain					815:820	a drastically truncated lectin domain	784:820	a drastically truncated lectin domain	784:820	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	6	6	theme	Cryo-EM	934:940	arg1	analysis					964:971	Cryo-EM and mass spectrometry analysis	934:971	analysis	964:971	Cryo-EM and mass spectrometry analysis reveals a putative glycan shield on the now redundant lectin domain.					
32938911	7	7	dep	small	1190:1194	arg1	glycosylated					1205:1216	glycosylated	1205:1216	glycosylated	1205:1216	The findings further our insight into the evolution and host adaptation of human embecoviruses, and demonstrate the utility of cryo-EM for studying small, heavily glycosylated proteins.					
32938911	5	8	theme	intact	926:931	arg1	domain					914:919	the esterase domain	901:919	the esterase domain left intact	901:931	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	6	9	theme	redundant	1017:1025	arg1	domain					1034:1039	the now redundant lectin domain	1009:1039	the now redundant lectin domain	1009:1039	Cryo-EM and mass spectrometry analysis reveals a putative glycan shield on the now redundant lectin domain.					
32938911	5	10	theme	existing	753:760	arg1	structures					765:774	existing HE structures	753:774	existing HE structures	753:774	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	5	11	dep	structure	875:883	arg1	the					871:873	the	871:873	the	871:873	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	1	12	dep	betacoronaviruses	148:164	arg1	betacoronaviruses					148:164	The human betacoronaviruses HKU1 and OC43	138:178	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus)	138:201	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus) arose from separate zoonotic introductions, OC43 relatively recently and HKU1 apparently much longer ago.					
32938911	1	12	dep	betacoronaviruses	148:164	arg1	OC43					175:178	OC43	175:178	OC43	175:178	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus) arose from separate zoonotic introductions, OC43 relatively recently and HKU1 apparently much longer ago.					
32938911	1	12	dep	betacoronaviruses	148:164	arg1	Embecovirus					190:200	subgenus Embecovirus	181:200	subgenus Embecovirus	181:200	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus) arose from separate zoonotic introductions, OC43 relatively recently and HKU1 apparently much longer ago.					
32938911	1	12	dep	betacoronaviruses	148:164	arg1	HKU1					166:169	HKU1	166:169	HKU1	166:169	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus) arose from separate zoonotic introductions, OC43 relatively recently and HKU1 apparently much longer ago.					
32938911	5	13	theme	HE	762:763	arg1	structures					765:774	existing HE structures	753:774	existing HE structures	753:774	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	4	14	theme	cryo-EM	653:659	arg1	structure					661:669	the cryo-EM structure	649:669	the cryo-EM structure of the ~80 kDa, heavily glycosylated HKU1 HE at 3.4 Å resolution	649:734	Here we present the cryo-EM structure of the ~80 kDa, heavily glycosylated HKU1 HE at 3.4 Å resolution.					
32938911	7	15	theme	cryo-EM	1169:1175	arg1	utility					1158:1164	the utility	1154:1164	the utility of cryo-EM for studying small, heavily glycosylated proteins	1154:1225	The findings further our insight into the evolution and host adaptation of human embecoviruses, and demonstrate the utility of cryo-EM for studying small, heavily glycosylated proteins.					
32938911	2	16	theme	Embecovirus	309:319	arg1	particles					321:329	Embecovirus particles	309:329	Embecovirus particles	309:329	Embecovirus particles contain two surface projections called spike (S) and haemagglutinin-esterase (HE), with S mediating receptor binding and membrane fusion, and HE acting as a receptor-destroying enzyme.					
32938911	7	17	theme	human	1117:1121	arg1	embecoviruses					1123:1135	human embecoviruses	1117:1135	human embecoviruses	1117:1135	The findings further our insight into the evolution and host adaptation of human embecoviruses, and demonstrate the utility of cryo-EM for studying small, heavily glycosylated proteins.					
32938911	2	18	theme	receptor	431:438	arg1	binding					440:446	receptor binding	431:446	receptor binding	431:446	Embecovirus particles contain two surface projections called spike (S) and haemagglutinin-esterase (HE), with S mediating receptor binding and membrane fusion, and HE acting as a receptor-destroying enzyme.					
32938911	3	19	theme	dynamic	539:545	arg1	attachment					554:563	dynamic virion attachment	539:563	dynamic virion attachment	539:563	Together, they promote dynamic virion attachment to glycan-based receptors, specifically 9-O-acetylated sialic acid.					
32938911	7	20	theme	embecoviruses	1123:1135	arg1	evolution					1084:1092	evolution	1084:1092	evolution	1084:1092	The findings further our insight into the evolution and host adaptation of human embecoviruses, and demonstrate the utility of cryo-EM for studying small, heavily glycosylated proteins.					
32938911	7	20	theme	embecoviruses	1123:1135	arg1	host					1098:1101	host	1098:1101	host	1098:1101	The findings further our insight into the evolution and host adaptation of human embecoviruses, and demonstrate the utility of cryo-EM for studying small, heavily glycosylated proteins.					
32938911	3	21	theme	virion	547:552	arg1	attachment					554:563	dynamic virion attachment	539:563	dynamic virion attachment	539:563	Together, they promote dynamic virion attachment to glycan-based receptors, specifically 9-O-acetylated sialic acid.					
32938911	4	22	theme	~80 kDa	678:684	arg1	HE					713:714	the ~80 kDa, heavily glycosylated HKU1 HE	674:714	the ~80 kDa, heavily glycosylated HKU1 HE	674:714	Here we present the cryo-EM structure of the ~80 kDa, heavily glycosylated HKU1 HE at 3.4 Å resolution.					
32938911	5	23	theme	sialic	841:846	arg1	binding					853:859	sialic acid binding	841:859	sialic acid binding	841:859	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	0	24	theme	Cryo-EM	0:6	arg1	structure					8:16	Cryo-EM structure	0:16	Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase	0:60	Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans.					
32938911	2	25	theme	surface	343:349	arg1	projections					351:361	two surface projections	339:361	two surface projections called spike (S) and haemagglutinin-esterase (HE)	339:411	Embecovirus particles contain two surface projections called spike (S) and haemagglutinin-esterase (HE), with S mediating receptor binding and membrane fusion, and HE acting as a receptor-destroying enzyme.					
32938911	0	26	theme	coronavirus-HKU1	21:36	arg1	esterase					53:60	coronavirus-HKU1 haemagglutinin esterase	21:60	coronavirus-HKU1 haemagglutinin esterase	21:60	Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans.					
32938911	5	27	theme	acid	848:851	arg1	binding					853:859	sialic acid binding	841:859	sialic acid binding	841:859	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	3	28	theme	glycan-based	568:579	arg1	receptors					581:589	glycan-based receptors	568:589	glycan-based receptors	568:589	Together, they promote dynamic virion attachment to glycan-based receptors, specifically 9-O-acetylated sialic acid.					
32938911	1	29	theme	separate	214:221	arg1	introductions					232:244	separate zoonotic introductions	214:244	separate zoonotic introductions	214:244	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus) arose from separate zoonotic introductions, OC43 relatively recently and HKU1 apparently much longer ago.					
32938911	7	30	theme	small	1190:1194	arg1	proteins					1218:1225	small, heavily glycosylated proteins	1190:1225	small, heavily glycosylated proteins	1190:1225	The findings further our insight into the evolution and host adaptation of human embecoviruses, and demonstrate the utility of cryo-EM for studying small, heavily glycosylated proteins.					
32938911	1	31	theme	zoonotic	223:230	arg1	introductions					232:244	separate zoonotic introductions	214:244	separate zoonotic introductions	214:244	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus) arose from separate zoonotic introductions, OC43 relatively recently and HKU1 apparently much longer ago.					
32938911	0	32	theme	esterase	53:60	arg1	structure					8:16	Cryo-EM structure	0:16	Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase	0:60	Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans.					
32938911	6	33	theme	putative	983:990	arg1	glycan					992:997	a putative glycan	981:997	a putative glycan shield on the now redundant lectin domain	981:1039	Cryo-EM and mass spectrometry analysis reveals a putative glycan shield on the now redundant lectin domain.					
32938911	0	34	theme	haemagglutinin	38:51	arg1	esterase					53:60	coronavirus-HKU1 haemagglutinin esterase	21:60	coronavirus-HKU1 haemagglutinin esterase	21:60	Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans.					
32938911	2	35	contain	contain	331:337	arg2	projections					351:361	two surface projections	339:361	two surface projections called spike (S) and haemagglutinin-esterase (HE)	339:411	Embecovirus particles contain two surface projections called spike (S) and haemagglutinin-esterase (HE), with S mediating receptor binding and membrane fusion, and HE acting as a receptor-destroying enzyme.					
32938911	2	35	contain	contain	331:337	arg1	particles					321:329	Embecovirus particles	309:329	Embecovirus particles	309:329	Embecovirus particles contain two surface projections called spike (S) and haemagglutinin-esterase (HE), with S mediating receptor binding and membrane fusion, and HE acting as a receptor-destroying enzyme.					
32938911	2	36	theme	membrane	452:459	arg1	fusion					461:466	membrane fusion	452:466	membrane fusion	452:466	Embecovirus particles contain two surface projections called spike (S) and haemagglutinin-esterase (HE), with S mediating receptor binding and membrane fusion, and HE acting as a receptor-destroying enzyme.					
32938911	7	37	dep	evolution	1084:1092	arg1	adaptation					1103:1112	adaptation	1103:1112	adaptation	1103:1112	The findings further our insight into the evolution and host adaptation of human embecoviruses, and demonstrate the utility of cryo-EM for studying small, heavily glycosylated proteins.					
32938911	7	37	dep	evolution	1084:1092	arg1	the					1080:1082	the	1080:1082	the	1080:1082	The findings further our insight into the evolution and host adaptation of human embecoviruses, and demonstrate the utility of cryo-EM for studying small, heavily glycosylated proteins.					
32938911	0	38	theme	architectural	70:82	arg1	changes					84:90	architectural changes	70:90	architectural changes arising from prolonged circulation in humans	70:135	Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans.					
32938911	5	39	theme	esterase	905:912	arg1	domain					914:919	the esterase domain	901:919	the esterase domain left intact	901:931	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	6	40	theme	shield	999:1004	arg1	glycan					992:997	a putative glycan	981:997	a putative glycan shield on the now redundant lectin domain	981:1039	Cryo-EM and mass spectrometry analysis reveals a putative glycan shield on the now redundant lectin domain.					
32938911	3	41	theme	9-O-acetylated	605:618	arg1	acid					627:630	9-O-acetylated sialic acid	605:630	9-O-acetylated sialic acid	605:630	Together, they promote dynamic virion attachment to glycan-based receptors, specifically 9-O-acetylated sialic acid.					
32938911	3	42	theme	sialic	620:625	arg1	acid					627:630	9-O-acetylated sialic acid	605:630	9-O-acetylated sialic acid	605:630	Together, they promote dynamic virion attachment to glycan-based receptors, specifically 9-O-acetylated sialic acid.					
32938911	1	43	theme	human	142:146	arg1	betacoronaviruses					148:164	The human betacoronaviruses HKU1 and OC43	138:178	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus)	138:201	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus) arose from separate zoonotic introductions, OC43 relatively recently and HKU1 apparently much longer ago.					
32938911	1	43	theme	human	142:146	arg1	OC43					175:178	OC43	175:178	OC43	175:178	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus) arose from separate zoonotic introductions, OC43 relatively recently and HKU1 apparently much longer ago.					
32938911	1	43	theme	human	142:146	arg1	HKU1					166:169	HKU1	166:169	HKU1	166:169	The human betacoronaviruses HKU1 and OC43 (subgenus Embecovirus) arose from separate zoonotic introductions, OC43 relatively recently and HKU1 apparently much longer ago.					
32938911	6	44	theme	spectrometry	951:962	arg1	analysis					964:971	Cryo-EM and mass spectrometry analysis	934:971	analysis	964:971	Cryo-EM and mass spectrometry analysis reveals a putative glycan shield on the now redundant lectin domain.					
32938911	5	45	with	incompatible	823:834	arg1	function					889:896	function	889:896	function	889:896	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	5	45	with	incompatible	823:834	arg1	structure					875:883	structure	875:883	structure	875:883	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	5	45	with	incompatible	823:834	arg1	binding					853:859	sialic acid binding	841:859	sialic acid binding	841:859	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	5	46	theme	domain	914:919	arg1	function					889:896	function	889:896	function	889:896	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	5	46	theme	domain	914:919	arg1	structure					875:883	structure	875:883	structure	875:883	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	0	47	from	circulation	115:125	arg1	humans					130:135	humans	130:135	humans	130:135	Cryo-EM structure of coronavirus-HKU1 haemagglutinin esterase reveals architectural changes arising from prolonged circulation in humans.					
32938911	5	48	with	Comparison	737:746	arg1	structures					765:774	existing HE structures	753:774	existing HE structures	753:774	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	4	49	theme	HE	713:714	arg1	structure					661:669	the cryo-EM structure	649:669	the cryo-EM structure of the ~80 kDa, heavily glycosylated HKU1 HE at 3.4 Å resolution	649:734	Here we present the cryo-EM structure of the ~80 kDa, heavily glycosylated HKU1 HE at 3.4 Å resolution.					
32938911	4	50	from	resolution	725:734	arg1	structure					661:669	the cryo-EM structure	649:669	the cryo-EM structure of the ~80 kDa, heavily glycosylated HKU1 HE at 3.4 Å resolution	649:734	Here we present the cryo-EM structure of the ~80 kDa, heavily glycosylated HKU1 HE at 3.4 Å resolution.					
32938911	5	51	theme	incompatible	823:834	arg1	domain					815:820	a drastically truncated lectin domain	784:820	a drastically truncated lectin domain	784:820	Comparison with existing HE structures reveals a drastically truncated lectin domain, incompatible with sialic acid binding, but with the structure and function of the esterase domain left intact.					
32938911	6	52	theme	mass	946:949	arg1	analysis					964:971	Cryo-EM and mass spectrometry analysis	934:971	analysis	964:971	Cryo-EM and mass spectrometry analysis reveals a putative glycan shield on the now redundant lectin domain.					
32938911	4	53	dep	~80 kDa	678:684	arg1	glycosylated					695:706	glycosylated	695:706	glycosylated	695:706	Here we present the cryo-EM structure of the ~80 kDa, heavily glycosylated HKU1 HE at 3.4 Å resolution.					
32938911	4	54	theme	HKU1	708:711	arg1	HE					713:714	the ~80 kDa, heavily glycosylated HKU1 HE	674:714	the ~80 kDa, heavily glycosylated HKU1 HE	674:714	Here we present the cryo-EM structure of the ~80 kDa, heavily glycosylated HKU1 HE at 3.4 Å resolution.					
32940034	7	0	theme	2	1507:1507	arg1	factor					1497:1502	a factor	1495:1502	a factor of 2	1495:1507	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	6	1	theme	NGLs	1089:1092	arg1	efficiencies					1052:1063	The incorporation efficiencies	1034:1063	The incorporation efficiencies (into nanodiscs) of the NGLs	1034:1092	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	6	1	theme	NGLs	1089:1092	arg1	affinities					1104:1113	their affinities	1098:1113	their affinities for a fragment of family 51 carbohydrate-binding module (CBM)	1098:1175	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	8	2	theme	corresponding	1619:1631	arg1	NGLs					1633:1636	the corresponding NGLs	1615:1636	the corresponding NGLs of lactose	1615:1647	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	3	3	theme	GBP-GSL	568:574	arg1	interactions					576:587	biologically relevant GBP-GSL interactions	546:587	biologically relevant GBP-GSL interactions	546:587	The limited availability of purified GSLs represents a significant challenge to the discovery and characterization of biologically relevant GBP-GSL interactions.					
32940034	1	4	theme	normal	265:270	arg1	processes					295:303	normal and pathophysiological processes	265:303	normal and pathophysiological processes	265:303	Interactions between glycan-binding proteins (GBPs) and glycosphingolipids (GSLs) in the membranes of cells are implicated in a wide variety of normal and pathophysiological processes.					
32940034	7	5	theme	GM1	1428:1430	arg1	ganglioside					1432:1442	the GM1 ganglioside	1424:1442	the GM1 ganglioside pentasaccharide	1424:1458	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	8	6	theme	GM1	1684:1686	arg1	pentasaccharides					1696:1711	the GM1 and GD2 pentasaccharides	1680:1711	pentasaccharides	1696:1711	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	5	7	theme	A	882:882	arg1	trisaccharides					890:903	the blood group type A and B trisaccharides	861:903	the blood group type A and B trisaccharides	861:903	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	7	8	theme	similar	1479:1485	arg1	affinities					1468:1477	affinities	1468:1477	affinities similar, within a factor of 2, to those of native GM1	1468:1531	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	6	9	theme	carbohydrate-binding	1143:1162	arg1	CBM					1172:1174	CBM	1172:1174	CBM	1172:1174	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	6	9	theme	carbohydrate-binding	1143:1162	arg1	module					1164:1169	family 51 carbohydrate-binding module	1133:1169	family 51 carbohydrate-binding module (CBM)	1133:1175	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	4	10	theme	ionization	711:720	arg1	spectrometry					727:738	catch-and-release-electrospray ionization mass spectrometry	680:738	catch-and-release-electrospray ionization mass spectrometry	680:738	The present work investigates the use of neoglycolipids (NGLs) as surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.					
32940034	6	11	theme	lipid	1254:1258	arg1	structure					1260:1268	the optimal lipid structure	1242:1268	the optimal lipid structure	1242:1268	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	6	11	theme	lipid	1254:1258	arg1	moiety					1232:1237	an amide-linked 1,3-di-O-hexadecyl-glycerol moiety	1188:1237	an amide-linked 1,3-di-O-hexadecyl-glycerol moiety	1188:1237	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	2	12	theme	critical	318:325	arg1	roles					338:342	the critical biological roles	314:342	the critical biological roles these interactions play	314:366	Despite the critical biological roles these interactions play, the GSL ligands of most GBPs have not yet been identified.					
32940034	8	13	theme	GD2	1692:1694	arg1	pentasaccharides					1696:1711	the GM1 and GD2 pentasaccharides	1680:1711	pentasaccharides	1696:1711	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	5	14	theme	B	888:888	arg1	trisaccharides					890:903	the blood group type A and B trisaccharides	861:903	the blood group type A and B trisaccharides	861:903	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	5	15	with	pairs	838:842	arg1	residue					987:993	"ring-closed" monosaccharide residue	958:993	"ring-closed" monosaccharide residue at the reducing end	958:1013	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	5	15	with	pairs	838:842	arg1	groups					938:943	three different lipid head groups	911:943	three different lipid head groups	911:943	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	8	16	theme	ligands	1852:1858	arg1	discovery					1835:1843	the discovery	1831:1843	the discovery of GSL ligands of GBPs	1831:1866	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	1	17	theme	glycan-binding	142:155	arg1	proteins					157:164	glycan-binding proteins	142:164	glycan-binding proteins (GBPs)	142:171	Interactions between glycan-binding proteins (GBPs) and glycosphingolipids (GSLs) in the membranes of cells are implicated in a wide variety of normal and pathophysiological processes.					
32940034	1	17	theme	glycan-binding	142:155	arg1	GBPs					167:170	GBPs	167:170	GBPs	167:170	Interactions between glycan-binding proteins (GBPs) and glycosphingolipids (GSLs) in the membranes of cells are implicated in a wide variety of normal and pathophysiological processes.					
32940034	6	18	theme	optimal	1246:1252	arg1	structure					1260:1268	the optimal lipid structure	1242:1268	the optimal lipid structure	1242:1268	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	6	18	theme	optimal	1246:1252	arg1	moiety					1232:1237	an amide-linked 1,3-di-O-hexadecyl-glycerol moiety	1188:1237	an amide-linked 1,3-di-O-hexadecyl-glycerol moiety	1188:1237	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	8	19	theme	GBPs	1863:1866	arg1	ligands					1852:1858	GSL ligands	1848:1858	GSL ligands of GBPs	1848:1866	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	5	20	theme	group	871:875	arg1	A					882:882	the blood group type A	861:882	the blood group type A	861:882	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	4	21	theme	-based	752:757	arg1	screening					759:767	catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening	680:767	catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening	680:767	The present work investigates the use of neoglycolipids (NGLs) as surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.					
32940034	8	22	theme	family	1739:1744	arg1	CBM					1749:1751	the family 51 CBM	1735:1751	the family 51 CBM	1735:1751	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	5	23	theme	lipid	927:931	arg1	groups					938:943	three different lipid head groups	911:943	three different lipid head groups	911:943	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	6	24	theme	1,3-di-O-hexadecyl-glycerol	1204:1230	arg1	structure					1260:1268	the optimal lipid structure	1242:1268	the optimal lipid structure	1242:1268	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	6	24	theme	1,3-di-O-hexadecyl-glycerol	1204:1230	arg1	moiety					1232:1237	an amide-linked 1,3-di-O-hexadecyl-glycerol moiety	1188:1237	an amide-linked 1,3-di-O-hexadecyl-glycerol moiety	1188:1237	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	8	25	theme	human	1754:1758	arg1	galectin-7					1760:1769	human galectin-7	1754:1769	human galectin-7	1754:1769	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	0	26	theme	Mass	102:105	arg1	Spectrometry					107:118	Native Mass Spectrometry	95:118	Native Mass Spectrometry	95:118	Neoglycolipids as Glycosphingolipid Surrogates for Protein Binding Studies Using Nanodiscs and Native Mass Spectrometry.					
32940034	5	27	theme	"	970:970	arg1	residue					987:993	"ring-closed" monosaccharide residue	958:993	"ring-closed" monosaccharide residue at the reducing end	958:1013	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	3	28	theme	limited	432:438	arg1	availability					440:451	The limited availability	428:451	The limited availability of purified GSLs	428:468	The limited availability of purified GSLs represents a significant challenge to the discovery and characterization of biologically relevant GBP-GSL interactions.					
32940034	2	29	theme	most	388:391	arg1	GBPs					393:396	most GBPs	388:396	most GBPs	388:396	Despite the critical biological roles these interactions play, the GSL ligands of most GBPs have not yet been identified.					
32940034	3	30	dep	discovery	512:520	arg1	the					508:510	the	508:510	the	508:510	The limited availability of purified GSLs represents a significant challenge to the discovery and characterization of biologically relevant GBP-GSL interactions.					
32940034	3	31	theme	GSLs	465:468	arg1	availability					440:451	The limited availability	428:451	The limited availability of purified GSLs	428:468	The limited availability of purified GSLs represents a significant challenge to the discovery and characterization of biologically relevant GBP-GSL interactions.					
32940034	0	32	theme	Glycosphingolipid	18:34	arg1	Surrogates					36:45	Glycosphingolipid Surrogates	18:45	Glycosphingolipid Surrogates for Protein Binding Studies Using Nanodiscs and Native Mass Spectrometry	18:118	Neoglycolipids as Glycosphingolipid Surrogates for Protein Binding Studies Using Nanodiscs and Native Mass Spectrometry.					
32940034	1	33	theme	processes	295:303	arg1	processes					295:303	normal and pathophysiological processes	265:303	normal and pathophysiological processes	265:303	Interactions between glycan-binding proteins (GBPs) and glycosphingolipids (GSLs) in the membranes of cells are implicated in a wide variety of normal and pathophysiological processes.					
32940034	1	33	theme	processes	295:303	arg1	variety					254:260	a wide variety	247:260	a wide variety of normal and pathophysiological processes	247:303	Interactions between glycan-binding proteins (GBPs) and glycosphingolipids (GSLs) in the membranes of cells are implicated in a wide variety of normal and pathophysiological processes.					
32940034	6	34	theme	incorporation	1038:1050	arg1	efficiencies					1052:1063	The incorporation efficiencies	1034:1063	The incorporation efficiencies (into nanodiscs) of the NGLs	1034:1092	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	7	35	theme	lipid	1407:1411	arg1	moiety					1413:1418	the optimal lipid moiety	1395:1418	the optimal lipid moiety	1395:1418	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	7	36	theme	native	1522:1527	arg1	GM1					1529:1531	native GM1	1522:1531	native GM1	1522:1531	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	7	37	theme	B	1319:1319	arg1	CTB5					1343:1346	CTB5	1343:1346	CTB5	1343:1346	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	7	37	theme	B	1319:1319	arg1	homopentamer					1329:1340	cholera toxin B subunit homopentamer	1305:1340	cholera toxin B subunit homopentamer (CTB5)	1305:1347	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	8	38	theme	optimal	1568:1574	arg1	A					1576:1576	the optimal A	1564:1576	the optimal A	1564:1576	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	7	39	dep	ganglioside	1432:1442	arg1	pentasaccharide					1444:1458	pentasaccharide	1444:1458	the GM1 ganglioside pentasaccharide	1424:1458	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	0	40	theme	Binding	59:65	arg1	Studies					67:73	Protein Binding Studies	51:73	Protein Binding Studies Using Nanodiscs and Native Mass Spectrometry	51:118	Neoglycolipids as Glycosphingolipid Surrogates for Protein Binding Studies Using Nanodiscs and Native Mass Spectrometry.					
32940034	7	41	theme	cholera	1305:1311	arg1	toxin					1313:1317	cholera toxin	1305:1317	cholera toxin B subunit homopentamer (CTB5)	1305:1347	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	4	42	theme	ligands	823:829	arg1	discovery					806:814	the discovery	802:814	the discovery of GSL ligands	802:829	The present work investigates the use of neoglycolipids (NGLs) as surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.					
32940034	1	43	from	Interactions	121:132	arg1	membranes					210:218	the membranes	206:218	the membranes of cells	206:227	Interactions between glycan-binding proteins (GBPs) and glycosphingolipids (GSLs) in the membranes of cells are implicated in a wide variety of normal and pathophysiological processes.					
32940034	7	44	contain	containing	1363:1372	arg1	nanodiscs					1353:1361	nanodiscs	1353:1361	nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide	1353:1458	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	7	44	contain	containing	1363:1372	arg2	NGL					1377:1379	an NGL	1374:1379	an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide	1374:1458	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	8	45	theme	trisaccharide	1584:1596	arg1	NGLs					1598:1601	the optimal A and B trisaccharide NGLs	1564:1601	the optimal A and B trisaccharide NGLs	1564:1601	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	8	46	theme	lactose	1641:1647	arg1	NGLs					1633:1636	the corresponding NGLs	1615:1636	the corresponding NGLs of lactose	1615:1647	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	8	46	theme	lactose	1641:1647	arg1	pentasaccharides					1696:1711	the GM1 and GD2 pentasaccharides	1680:1711	pentasaccharides	1696:1711	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	8	46	theme	lactose	1641:1647	arg1	nanodiscs					1543:1551	nanodiscs	1543:1551	nanodiscs containing the optimal A and B trisaccharide NGLs	1543:1601	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	8	46	theme	lactose	1641:1647	arg1	tetrasaccharide					1659:1673	A type 2 tetrasaccharide	1650:1673	A type 2 tetrasaccharide	1650:1673	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	7	47	theme	Binding	1271:1277	arg1	measurements					1279:1290	Binding measurements	1271:1290	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide	1271:1458	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	8	48	theme	NGLs	1812:1815	arg1	potential					1799:1807	the potential	1795:1807	the potential of NGLs to accelerate the discovery of GSL ligands of GBPs	1795:1866	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	1	49	theme	wide	249:252	arg1	processes					295:303	normal and pathophysiological processes	265:303	normal and pathophysiological processes	265:303	Interactions between glycan-binding proteins (GBPs) and glycosphingolipids (GSLs) in the membranes of cells are implicated in a wide variety of normal and pathophysiological processes.					
32940034	1	49	theme	wide	249:252	arg1	variety					254:260	a wide variety	247:260	a wide variety of normal and pathophysiological processes	247:303	Interactions between glycan-binding proteins (GBPs) and glycosphingolipids (GSLs) in the membranes of cells are implicated in a wide variety of normal and pathophysiological processes.					
32940034	3	50	theme	relevant	559:566	arg1	interactions					576:587	biologically relevant GBP-GSL interactions	546:587	biologically relevant GBP-GSL interactions	546:587	The limited availability of purified GSLs represents a significant challenge to the discovery and characterization of biologically relevant GBP-GSL interactions.					
32940034	5	51	from	end	1011:1013	arg1	residue					987:993	"ring-closed" monosaccharide residue	958:993	"ring-closed" monosaccharide residue at the reducing end	958:1013	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	5	52	theme	type	877:880	arg1	A					882:882	the blood group type A	861:882	the blood group type A	861:882	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	6	53	theme	module	1164:1169	arg1	fragment					1121:1128	a fragment	1119:1128	a fragment of family 51 carbohydrate-binding module (CBM)	1119:1175	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	8	54	theme	GSL	1848:1850	arg1	ligands					1852:1858	GSL ligands	1848:1858	GSL ligands of GBPs	1848:1866	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	2	55	theme	biological	327:336	arg1	roles					338:342	the critical biological roles	314:342	the critical biological roles these interactions play	314:366	Despite the critical biological roles these interactions play, the GSL ligands of most GBPs have not yet been identified.					
32940034	4	56	theme	catch-and-release-electrospray	680:709	arg1	spectrometry					727:738	catch-and-release-electrospray ionization mass spectrometry	680:738	catch-and-release-electrospray ionization mass spectrometry	680:738	The present work investigates the use of neoglycolipids (NGLs) as surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.					
32940034	3	57	theme	interactions	576:587	arg1	discovery					512:520	discovery	512:520	discovery	512:520	The limited availability of purified GSLs represents a significant challenge to the discovery and characterization of biologically relevant GBP-GSL interactions.					
32940034	3	57	theme	interactions	576:587	arg1	characterization					526:541	characterization	526:541	characterization	526:541	The limited availability of purified GSLs represents a significant challenge to the discovery and characterization of biologically relevant GBP-GSL interactions.					
32940034	5	58	theme	blood	865:869	arg1	A					882:882	the blood group type A	861:882	the blood group type A	861:882	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	7	59	theme	optimal	1399:1405	arg1	moiety					1413:1418	the optimal lipid moiety	1395:1418	the optimal lipid moiety	1395:1418	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	5	60	theme	different	917:925	arg1	groups					938:943	three different lipid head groups	911:943	three different lipid head groups	911:943	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	6	61	link	amide-linked	1191:1202	arg1	structure					1260:1268	the optimal lipid structure	1242:1268	the optimal lipid structure	1242:1268	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	6	61	link	amide-linked	1191:1202	arg1	moiety					1232:1237	an amide-linked 1,3-di-O-hexadecyl-glycerol moiety	1188:1237	an amide-linked 1,3-di-O-hexadecyl-glycerol moiety	1188:1237	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	5	62	theme	head	933:936	arg1	groups					938:943	three different lipid head groups	911:943	three different lipid head groups	911:943	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	6	63	theme	family	1133:1138	arg1	CBM					1172:1174	CBM	1172:1174	CBM	1172:1174	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	6	63	theme	family	1133:1138	arg1	module					1164:1169	family 51 carbohydrate-binding module	1133:1169	family 51 carbohydrate-binding module (CBM)	1133:1175	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	6	64	theme	amide-linked	1191:1202	arg1	structure					1260:1268	the optimal lipid structure	1242:1268	the optimal lipid structure	1242:1268	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	6	64	theme	amide-linked	1191:1202	arg1	moiety					1232:1237	an amide-linked 1,3-di-O-hexadecyl-glycerol moiety	1188:1237	an amide-linked 1,3-di-O-hexadecyl-glycerol moiety	1188:1237	The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragment of family 51 carbohydrate-binding module (CBM) identified an amide-linked 1,3-di-O-hexadecyl-glycerol moiety as the optimal lipid structure.					
32940034	8	65	contain	containing	1553:1562	arg1	nanodiscs					1543:1551	nanodiscs	1543:1551	nanodiscs containing the optimal A and B trisaccharide NGLs	1543:1601	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	8	65	contain	containing	1553:1562	arg2	NGLs					1598:1601	the optimal A and B trisaccharide NGLs	1564:1601	the optimal A and B trisaccharide NGLs	1564:1601	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	4	66	theme	mass	722:725	arg1	spectrometry					727:738	catch-and-release-electrospray ionization mass spectrometry	680:738	catch-and-release-electrospray ionization mass spectrometry	680:738	The present work investigates the use of neoglycolipids (NGLs) as surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.					
32940034	0	67	theme	Native	95:100	arg1	Spectrometry					107:118	Native Mass Spectrometry	95:118	Native Mass Spectrometry	95:118	Neoglycolipids as Glycosphingolipid Surrogates for Protein Binding Studies Using Nanodiscs and Native Mass Spectrometry.					
32940034	5	68	theme	ring-closed	959:969	arg1	residue					987:993	"ring-closed" monosaccharide residue	958:993	"ring-closed" monosaccharide residue at the reducing end	958:1013	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	5	69	theme	monosaccharide	972:985	arg1	residue					987:993	"ring-closed" monosaccharide residue	958:993	"ring-closed" monosaccharide residue at the reducing end	958:1013	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	2	70	theme	GBPs	393:396	arg1	ligands					377:383	the GSL ligands	369:383	the GSL ligands of most GBPs	369:396	Despite the critical biological roles these interactions play, the GSL ligands of most GBPs have not yet been identified.					
32940034	2	71	dep	roles	338:342	arg1	play					363:366	play	363:366	play	363:366	Despite the critical biological roles these interactions play, the GSL ligands of most GBPs have not yet been identified.					
32940034	5	72	theme	NGLs	847:850	arg1	pairs					838:842	Three pairs	832:842	Three pairs	832:842	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	1	73	theme	pathophysiological	276:293	arg1	processes					295:303	normal and pathophysiological processes	265:303	normal and pathophysiological processes	265:303	Interactions between glycan-binding proteins (GBPs) and glycosphingolipids (GSLs) in the membranes of cells are implicated in a wide variety of normal and pathophysiological processes.					
32940034	4	74	dep	-based	752:757	arg1	spectrometry					727:738	catch-and-release-electrospray ionization mass spectrometry	680:738	catch-and-release-electrospray ionization mass spectrometry	680:738	The present work investigates the use of neoglycolipids (NGLs) as surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.					
32940034	7	75	theme	subunit	1321:1327	arg1	CTB5					1343:1346	CTB5	1343:1346	CTB5	1343:1346	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	7	75	theme	subunit	1321:1327	arg1	homopentamer					1329:1340	cholera toxin B subunit homopentamer	1305:1340	cholera toxin B subunit homopentamer (CTB5)	1305:1347	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	4	76	theme	present	594:600	arg1	work					602:605	The present work	590:605	The present work	590:605	The present work investigates the use of neoglycolipids (NGLs) as surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.					
32940034	0	77	theme	Protein	51:57	arg1	Studies					67:73	Protein Binding Studies	51:73	Protein Binding Studies Using Nanodiscs and Native Mass Spectrometry	51:118	Neoglycolipids as Glycosphingolipid Surrogates for Protein Binding Studies Using Nanodiscs and Native Mass Spectrometry.					
32940034	7	78	theme	toxin	1313:1317	arg1	CTB5					1343:1346	CTB5	1343:1346	CTB5	1343:1346	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	7	78	theme	toxin	1313:1317	arg1	homopentamer					1329:1340	cholera toxin B subunit homopentamer	1305:1340	cholera toxin B subunit homopentamer (CTB5)	1305:1347	Binding measurements performed on cholera toxin B subunit homopentamer (CTB5) and nanodiscs containing an NGL consisting of the optimal lipid moiety and the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of native GM1.					
32940034	8	79	theme	A	1576:1576	arg1	NGLs					1598:1601	the optimal A and B trisaccharide NGLs	1564:1601	the optimal A and B trisaccharide NGLs	1564:1601	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	3	80	theme	purified	456:463	arg1	GSLs					465:468	purified GSLs	456:468	purified GSLs	456:468	The limited availability of purified GSLs represents a significant challenge to the discovery and characterization of biologically relevant GBP-GSL interactions.					
32940034	8	81	theme	B	1582:1582	arg1	NGLs					1598:1601	the optimal A and B trisaccharide NGLs	1564:1601	the optimal A and B trisaccharide NGLs	1564:1601	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
32940034	4	82	theme	neoglycolipids	631:644	arg1	surrogates					656:665	surrogates	656:665	surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands	656:829	The present work investigates the use of neoglycolipids (NGLs) as surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.					
32940034	4	82	theme	neoglycolipids	631:644	arg1	use					624:626	the use	620:626	the use of neoglycolipids (NGLs)	620:651	The present work investigates the use of neoglycolipids (NGLs) as surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.					
32940034	4	83	theme	GSL	819:821	arg1	ligands					823:829	GSL ligands	819:829	GSL ligands	819:829	The present work investigates the use of neoglycolipids (NGLs) as surrogates for GSLs for catch-and-release-electrospray ionization mass spectrometry (CaR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.					
32940034	3	84	theme	significant	483:493	arg1	challenge					495:503	a significant challenge	481:503	a significant challenge to the discovery and characterization of biologically relevant GBP-GSL interactions	481:587	The limited availability of purified GSLs represents a significant challenge to the discovery and characterization of biologically relevant GBP-GSL interactions.					
32940034	1	85	theme	cells	223:227	arg1	membranes					210:218	the membranes	206:218	the membranes of cells	206:227	Interactions between glycan-binding proteins (GBPs) and glycosphingolipids (GSLs) in the membranes of cells are implicated in a wide variety of normal and pathophysiological processes.					
32940034	5	86	theme	reducing	1002:1009	arg1	end					1011:1013	the reducing end	998:1013	the reducing end	998:1013	Three pairs of NGLs based on the blood group type A and B trisaccharides, with three different lipid head groups but all with "ring-closed" monosaccharide residue at the reducing end, were synthesized.					
32940034	2	87	theme	GSL	373:375	arg1	ligands					377:383	the GSL ligands	369:383	the GSL ligands of most GBPs	369:396	Despite the critical biological roles these interactions play, the GSL ligands of most GBPs have not yet been identified.					
32940034	8	88	theme	type	1652:1655	arg1	tetrasaccharide					1659:1673	A type 2 tetrasaccharide	1650:1673	A type 2 tetrasaccharide	1650:1673	Finally, nanodiscs containing the optimal A and B trisaccharide NGLs, as well as the corresponding NGLs of lactose, A type 2 tetrasaccharide, and the GM1 and GD2 pentasaccharides were screened against the family 51 CBM, human galectin-7, and CTB5 to illustrate the potential of NGLs to accelerate the discovery of GSL ligands of GBPs.					
33937925	0	0	theme	trivalent	130:138	arg1	vaccine					159:165	a trivalent seasonal influenza vaccine	128:165	a trivalent seasonal influenza vaccine	128:165	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	8	1	theme	glycans	1495:1501	arg1	glycans					1495:1501	glycans	1495:1501	glycans	1495:1501	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	8	1	theme	glycans	1495:1501	arg1	group					1486:1490	the most abundant group	1468:1490	the most abundant group of glycans	1468:1501	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	0	2	theme	vaccine	159:165	arg1	production					114:123	the production	110:123	the production of a trivalent seasonal influenza vaccine	110:165	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	2	3	theme	Chicken	326:332	arg1	host					373:376	the predominant production host	346:376	the predominant production host for influenza vaccines	346:399	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	2	3	theme	Chicken	326:332	arg1	embryos					334:340	Chicken embryos	326:340	Chicken embryos	326:340	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	0	4	link	N-linked	14:21	arg1	glycosylation					23:35	N-linked glycosylation	14:35	N-linked glycosylation	14:35	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	3	5	theme	viruses	684:690	arg1	strains					663:669	three strains	657:669	three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###)	657:908	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	6	dep	###	905:907	arg1	i.e.					790:793	i.e.	790:793	i.e.	790:793	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	7	dep	the	646:648	arg1	HAs					650:652	HAs	650:652	HAs	650:652	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	5	8	dep	embryo-derived	1147:1160	arg1	HAs					1162:1164	HAs	1162:1164	HAs	1162:1164	For the H3N2 and B strains, MDCK-derived HAs contained more sites being partially occupied (<95%) than embryo-derived HAs.					
33937925	10	9	theme	occupied	1933:1940	arg1	sites					1956:1960	more partially (<95%) occupied glycosylation sites	1911:1960	more partially (<95%) occupied glycosylation sites	1911:1960	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	2	10	theme	kidney	425:430	arg1	cells					439:443	Madin-Darby canine kidney (MDCK) cells	406:443	Madin-Darby canine kidney (MDCK) cells	406:443	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	3	11	gly	glycosylation	580:592	arg2	sites					594:598	potential glycosylation sites	570:598	potential glycosylation sites	570:598	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	1	12	from	antigen	295:301	arg1	vaccines					316:323	influenza vaccines	306:323	influenza vaccines	306:323	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	3	13	theme	glycosylation	519:531	arg1	occupancy					557:565	the occupancy	553:565	the occupancy of potential glycosylation sites	553:598	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	13	theme	glycosylation	519:531	arg1	patterns					533:540	glycosylation patterns	519:540	glycosylation patterns	519:540	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	13	theme	glycosylation	519:531	arg1	distribution					608:619	the distribution	604:619	the distribution of different glycans	604:640	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	7	14	contain	contain	1386:1392	arg1	MDCK-derived					1369:1380	MDCK-derived	1369:1380	MDCK-derived	1369:1380	In general, MDCK-derived HAs contain more glycans of higher molecular weight.					
33937925	7	14	contain	contain	1386:1392	arg1	general					1360:1366	general	1360:1366	general	1360:1366	In general, MDCK-derived HAs contain more glycans of higher molecular weight.					
33937925	7	14	contain	contain	1386:1392	arg2	glycans					1399:1405	more glycans	1394:1405	more glycans of higher molecular weight	1394:1432	In general, MDCK-derived HAs contain more glycans of higher molecular weight.					
33937925	7	14	contain	contain	1386:1392	arg2	MDCK-derived					1369:1380	MDCK-derived	1369:1380	MDCK-derived	1369:1380	In general, MDCK-derived HAs contain more glycans of higher molecular weight.					
33937925	5	15	dep	occupied	1126:1133	arg1	%					1139:1139	<95%	1136:1139	<95%	1136:1139	For the H3N2 and B strains, MDCK-derived HAs contained more sites being partially occupied (<95%) than embryo-derived HAs.					
33937925	0	16	theme	production	114:123	arg1	case					102:105	a case	100:105	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.	0:166	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	8	17	theme	lower	1599:1603	arg1	abundance					1605:1613	that lower abundance	1594:1613	that lower abundance	1594:1613	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	9	18	from	role	1781:1784	arg1	function					1810:1817	the function	1806:1817	the function of influenza vaccines	1806:1839	The different glycosylation patterns between MDCK- and chicken embryo-derived HAs may help elucidate the role of glycosylation on the function of influenza vaccines.					
33937925	11	19	dep	MDCK-derived	1965:1976	arg1	HAs					1978:1980	HAs	1978:1980	HAs	1978:1980	• MDCK-derived HAs contained more glycans of higher molecular weight.					
33937925	6	20	theme	sensitive	1176:1184	arg1	assay					1193:1197	A highly sensitive glycan assay	1167:1197	A highly sensitive glycan assay	1167:1197	A highly sensitive glycan assay was developed where 50 different glycans were identified, which was more than what has been reported previously, and their relative abundance was quantified.					
33937925	3	21	theme	H1N1	817:820	arg1	###					905:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	21	theme	H1N1	817:820	arg1	viruses					684:690	influenza viruses	674:690	influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###)	674:908	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	9	22	theme	influenza	1822:1830	arg1	vaccines					1832:1839	influenza vaccines	1822:1839	influenza vaccines	1822:1839	The different glycosylation patterns between MDCK- and chicken embryo-derived HAs may help elucidate the role of glycosylation on the function of influenza vaccines.					
33937925	3	23	theme	Northern	762:769	arg1	season					782:787	the 2015-2016 Northern Hemisphere season	748:787	the 2015-2016 Northern Hemisphere season	748:787	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	8	24	theme	structure	1624:1632	arg1	glycans					1634:1640	complex structure glycans	1616:1640	complex structure glycans	1616:1640	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	12	25	dep	•	2033:2033	arg1	used					2083:2086	used	2083:2086	HAs used for trivalent seasonal flu vaccines	2079:2122	• A systematic comparison of glycosylation on HAs used for trivalent seasonal flu vaccines was conducted.					
33937925	2	26	theme	important	464:472	arg1	host					486:489	an important alternative host	461:489	an important alternative host	461:489	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	0	27	theme	chicken	68:74	arg1	embryos					76:82	chicken embryos	68:82	chicken embryos	68:82	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	3	28	theme	glycosylation	580:592	arg1	sites					594:598	potential glycosylation sites	570:598	potential glycosylation sites	570:598	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	12	29	theme	seasonal	2102:2109	arg1	vaccines					2115:2122	trivalent seasonal flu vaccines	2092:2122	trivalent seasonal flu vaccines	2092:2122	• A systematic comparison of glycosylation on HAs used for trivalent seasonal flu vaccines was conducted.					
33937925	3	30	theme	X179A	823:827	arg1	###					905:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	30	theme	X179A	823:827	arg1	viruses					684:690	influenza viruses	674:690	influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###)	674:908	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	0	31	from	Comparison	0:9	arg1	hemagglutinins					40:53	hemagglutinins	40:53	hemagglutinins derived from chicken embryos and MDCK cells	40:97	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	9	32	theme	vaccines	1832:1839	arg1	function					1810:1817	the function	1806:1817	the function of influenza vaccines	1806:1839	The different glycosylation patterns between MDCK- and chicken embryo-derived HAs may help elucidate the role of glycosylation on the function of influenza vaccines.					
33937925	7	33	theme	molecular	1417:1425	arg1	weight					1427:1432	higher molecular weight	1410:1432	higher molecular weight	1410:1432	In general, MDCK-derived HAs contain more glycans of higher molecular weight.					
33937925	1	34	link	N-linked	168:175	arg1	glycosylation					177:189	N-linked glycosylation	168:189	N-linked glycosylation	168:189	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	5	35	theme	more	1099:1102	arg1	sites					1104:1108	more sites	1099:1108	more sites	1099:1108	For the H3N2 and B strains, MDCK-derived HAs contained more sites being partially occupied (<95%) than embryo-derived HAs.					
33937925	3	36	theme	H3N2	858:861	arg1	###					905:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	36	theme	H3N2	858:861	arg1	viruses					684:690	influenza viruses	674:690	influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###)	674:908	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	11	37	theme	more	1992:1995	arg1	glycans					1997:2003	more glycans	1992:2003	more glycans of higher molecular weight	1992:2030	• MDCK-derived HAs contained more glycans of higher molecular weight.					
33937925	2	38	theme	production	362:371	arg1	host					373:376	the predominant production host	346:376	the predominant production host for influenza vaccines	346:399	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	2	38	theme	production	362:371	arg1	embryos					334:340	Chicken embryos	326:340	Chicken embryos	326:340	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	1	39	theme	hemagglutinin	266:278	arg1	folding					215:221	folding	215:221	folding	215:221	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	1	39	theme	hemagglutinin	266:278	arg1	binding					233:239	receptor binding	224:239	receptor binding	224:239	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	1	39	theme	hemagglutinin	266:278	arg1	immunomodulating					246:261	immunomodulating	246:261	immunomodulating of hemagglutinin (HA)	246:283	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	1	39	theme	hemagglutinin	266:278	arg1	antigen					295:301	the main antigen	286:301	the main antigen in influenza vaccines	286:323	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	3	40	theme	NYMC	895:898	arg1	###					905:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	40	theme	NYMC	895:898	arg1	viruses					684:690	influenza viruses	674:690	influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###)	674:908	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	2	41	theme	canine	418:423	arg1	kidney					425:430	Madin-Darby canine kidney	406:430	Madin-Darby canine kidney (MDCK) cells	406:443	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	2	41	theme	canine	418:423	arg1	MDCK					433:436	MDCK	433:436	MDCK	433:436	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	12	42	theme	systematic	2037:2046	arg1	comparison					2048:2057	A systematic comparison	2035:2057	A systematic comparison of glycosylation on	2035:2077	• A systematic comparison of glycosylation on HAs used for trivalent seasonal flu vaccines was conducted.					
33937925	0	43	theme	N-linked	14:21	arg1	glycosylation					23:35	N-linked glycosylation	14:35	N-linked glycosylation	14:35	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	4	44	from	sites	956:960	arg1	the					965:967	the	965:967	the	965:967	Of the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively, most were highly occupied.					
33937925	11	45	theme	molecular	2015:2023	arg1	weight					2025:2030	higher molecular weight	2008:2030	higher molecular weight	2008:2030	• MDCK-derived HAs contained more glycans of higher molecular weight.					
33937925	3	46	theme	different	624:632	arg1	glycans					634:640	different glycans	624:640	different glycans	624:640	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	4	47	used	occupied	1034:1041	arg2	most					1017:1020	most	1017:1020	most	1017:1020	Of the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively, most were highly occupied.					
33937925	4	48	theme	potential	932:940	arg1	sites					956:960	the 8, 12, and 11 potential glycosylation sites	914:960	the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively	914:1014	Of the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively, most were highly occupied.					
33937925	8	49	theme	High-mannose	1435:1446	arg1	species					1448:1454	High-mannose species	1435:1454	High-mannose species	1435:1454	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	10	50	contain	contained	1901:1909	arg1	•					1854:1854	•	1854:1854	• For the H3N2 and B strains, MDCK-derived HAs	1854:1899	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	10	50	contain	contained	1901:1909	arg2	sites					1956:1960	more partially (<95%) occupied glycosylation sites	1911:1960	more partially (<95%) occupied glycosylation sites	1911:1960	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	3	51	dep	viruses	684:690	arg1	vaccine					736:742	a trivalent seasonal flu vaccine	711:742	influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###)	674:908	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	9	52	theme	glycosylation	1690:1702	arg1	patterns					1704:1711	The different glycosylation patterns	1676:1711	The different glycosylation patterns between MDCK- and chicken embryo-derived HAs	1676:1756	The different glycosylation patterns between MDCK- and chicken embryo-derived HAs may help elucidate the role of glycosylation on the function of influenza vaccines.					
33937925	11	53	contain	contained	1982:1990	arg2	glycans					1997:2003	more glycans	1992:2003	more glycans of higher molecular weight	1992:2030	• MDCK-derived HAs contained more glycans of higher molecular weight.					
33937925	11	53	contain	contained	1982:1990	arg1	MDCK-derived					1965:1976	MDCK-derived	1965:1976	MDCK-derived	1965:1976	• MDCK-derived HAs contained more glycans of higher molecular weight.					
33937925	4	54	theme	B	992:992	arg1	strains					994:1000	B strains	992:1000	B strains	992:1000	Of the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively, most were highly occupied.					
33937925	12	55	used	used	2083:2086	arg2	comparison					2048:2057	A systematic comparison	2035:2057	A systematic comparison of glycosylation on	2035:2077	• A systematic comparison of glycosylation on HAs used for trivalent seasonal flu vaccines was conducted.					
33937925	8	56	theme	lower	1513:1517	arg1	level					1519:1523	a lower level	1511:1523	a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study	1511:1673	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	1	57	theme	receptor	224:231	arg1	binding					233:239	receptor binding	224:239	receptor binding	224:239	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	0	58	dep	Comparison	0:9	arg1	case					102:105	a case	100:105	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.	0:166	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	8	59	theme	abundant	1477:1484	arg1	glycans					1495:1501	glycans	1495:1501	glycans	1495:1501	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	8	59	theme	abundant	1477:1484	arg1	group					1486:1490	the most abundant group	1468:1490	the most abundant group of glycans	1468:1501	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	6	60	theme	relative	1322:1329	arg1	abundance					1331:1339	their relative abundance	1316:1339	their relative abundance	1316:1339	A highly sensitive glycan assay was developed where 50 different glycans were identified, which was more than what has been reported previously, and their relative abundance was quantified.					
33937925	10	61	dep	partially	1916:1924	arg1	%					1930:1930	<95%	1927:1930	<95%	1927:1930	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	7	62	dep	MDCK-derived	1369:1380	arg1	HAs					1382:1384	HAs	1382:1384	HAs	1382:1384	In general, MDCK-derived HAs contain more glycans of higher molecular weight.					
33937925	10	63	theme	glycosylation	1942:1954	arg1	sites					1956:1960	more partially (<95%) occupied glycosylation sites	1911:1960	more partially (<95%) occupied glycosylation sites	1911:1960	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	4	64	dep	the	965:967	arg1	HAs					969:971	HAs	969:971	HAs	969:971	Of the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively, most were highly occupied.					
33937925	11	65	theme	higher	2008:2013	arg1	weight					2025:2030	higher molecular weight	2008:2030	higher molecular weight	2008:2030	• MDCK-derived HAs contained more glycans of higher molecular weight.					
33937925	5	66	contain	contained	1089:1097	arg1	MDCK-derived					1072:1083	MDCK-derived	1072:1083	MDCK-derived	1072:1083	For the H3N2 and B strains, MDCK-derived HAs contained more sites being partially occupied (<95%) than embryo-derived HAs.					
33937925	5	66	contain	contained	1089:1097	arg2	sites					1104:1108	more sites	1099:1108	more sites	1099:1108	For the H3N2 and B strains, MDCK-derived HAs contained more sites being partially occupied (<95%) than embryo-derived HAs.					
33937925	10	67	dep	MDCK-derived	1884:1895	arg1	HAs					1897:1899	HAs	1897:1899	HAs	1897:1899	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	1	68	theme	main	290:293	arg1	immunomodulating					246:261	immunomodulating	246:261	immunomodulating of hemagglutinin (HA)	246:283	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	1	68	theme	main	290:293	arg1	antigen					295:301	the main antigen	286:301	the main antigen in influenza vaccines	286:323	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	3	69	theme	influenza	674:682	arg1	###					905:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	69	theme	influenza	674:682	arg1	viruses					684:690	influenza viruses	674:690	influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###)	674:908	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	8	70	theme	previous	1558:1565	arg1	studies					1567:1573	previous studies	1558:1573	previous studies	1558:1573	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	1	71	theme	N-linked	168:175	arg1	glycosylation					177:189	N-linked glycosylation	168:189	N-linked glycosylation	168:189	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	0	72	attach	derived	55:61	arg2	hemagglutinins					40:53	hemagglutinins	40:53	hemagglutinins derived from chicken embryos and MDCK cells	40:97	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	0	72	attach	derived	55:61	arg1	embryos					76:82	chicken embryos	68:82	chicken embryos	68:82	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	0	72	attach	derived	55:61	arg1	cells					93:97	MDCK cells	88:97	MDCK cells	88:97	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	3	73	theme	trivalent	713:721	arg1	vaccine					736:742	a trivalent seasonal flu vaccine	711:742	influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###)	674:908	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	9	74	theme	glycosylation	1789:1801	arg1	role					1781:1784	the role	1777:1784	the role of glycosylation on the function of influenza vaccines	1777:1839	The different glycosylation patterns between MDCK- and chicken embryo-derived HAs may help elucidate the role of glycosylation on the function of influenza vaccines.					
33937925	3	75	gly	occupancy	557:565	arg2	sites					594:598	potential glycosylation sites	570:598	potential glycosylation sites	570:598	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	75	gly	occupancy	557:565	arg2	glycans					634:640	different glycans	624:640	different glycans	624:640	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	10	76	theme	KEY	1842:1844	arg1	POINTS					1846:1851	KEY POINTS	1842:1851	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.	1842:1961	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	6	77	theme	glycan	1186:1191	arg1	assay					1193:1197	A highly sensitive glycan assay	1167:1197	A highly sensitive glycan assay	1167:1197	A highly sensitive glycan assay was developed where 50 different glycans were identified, which was more than what has been reported previously, and their relative abundance was quantified.					
33937925	3	78	theme	flu	732:734	arg1	vaccine					736:742	a trivalent seasonal flu vaccine	711:742	influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###)	674:908	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	0	79	theme	influenza	149:157	arg1	vaccine					159:165	a trivalent seasonal influenza vaccine	128:165	a trivalent seasonal influenza vaccine	128:165	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	3	80	theme	sites	594:598	arg1	occupancy					557:565	the occupancy	553:565	the occupancy of potential glycosylation sites	553:598	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	80	theme	sites	594:598	arg1	distribution					608:619	the distribution	604:619	the distribution of different glycans	604:640	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	0	81	dep	trivalent	130:138	arg1	seasonal					140:147	seasonal	140:147	seasonal	140:147	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	5	82	dep	MDCK-derived	1072:1083	arg1	HAs					1085:1087	HAs	1085:1087	HAs	1085:1087	For the H3N2 and B strains, MDCK-derived HAs contained more sites being partially occupied (<95%) than embryo-derived HAs.					
33937925	8	83	dep	level	1519:1523	arg1	compared					1528:1535	compared	1528:1535	compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study	1528:1673	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	3	84	from	patterns	533:540	arg1	the					646:648	the	646:648	the	646:648	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	85	theme	Hemisphere	771:780	arg1	season					782:787	the 2015-2016 Northern Hemisphere season	748:787	the 2015-2016 Northern Hemisphere season	748:787	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	86	theme	potential	570:578	arg1	sites					594:598	potential glycosylation sites	570:598	potential glycosylation sites	570:598	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	8	87	theme	complex	1616:1622	arg1	glycans					1634:1640	complex structure glycans	1616:1640	complex structure glycans	1616:1640	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	12	88	theme	flu	2111:2113	arg1	vaccines					2115:2122	trivalent seasonal flu vaccines	2092:2122	trivalent seasonal flu vaccines	2092:2122	• A systematic comparison of glycosylation on HAs used for trivalent seasonal flu vaccines was conducted.					
33937925	3	89	theme	2015-2016	752:760	arg1	season					782:787	the 2015-2016 Northern Hemisphere season	748:787	the 2015-2016 Northern Hemisphere season	748:787	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	0	90	theme	MDCK	88:91	arg1	cells					93:97	MDCK cells	88:97	MDCK cells	88:97	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	1	91	theme	influenza	306:314	arg1	vaccines					316:323	influenza vaccines	306:323	influenza vaccines	306:323	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	7	92	theme	weight	1427:1432	arg1	glycans					1399:1405	more glycans	1394:1405	more glycans of higher molecular weight	1394:1432	In general, MDCK-derived HAs contain more glycans of higher molecular weight.					
33937925	10	93	dep	POINTS	1846:1851	arg1	contained					1901:1909	contained	1901:1909	contained more partially (<95%) occupied glycosylation sites	1901:1960	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	10	94	theme	B	1873:1873	arg1	MDCK-derived					1884:1895	MDCK-derived	1884:1895	MDCK-derived	1884:1895	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	10	94	theme	B	1873:1873	arg1	strains					1875:1881	the H3N2 and B strains	1860:1881	the H3N2 and B strains	1860:1881	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	3	95	dep	trivalent	713:721	arg1	seasonal					723:730	seasonal	723:730	seasonal	723:730	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	9	96	dep	MDCK-	1721:1725	arg1	HAs					1754:1756	HAs	1754:1756	HAs	1754:1756	The different glycosylation patterns between MDCK- and chicken embryo-derived HAs may help elucidate the role of glycosylation on the function of influenza vaccines.					
33937925	7	97	theme	higher	1410:1415	arg1	weight					1427:1432	higher molecular weight	1410:1432	higher molecular weight	1410:1432	In general, MDCK-derived HAs contain more glycans of higher molecular weight.					
33937925	10	98	theme	H3N2	1864:1867	arg1	MDCK-derived					1884:1895	MDCK-derived	1884:1895	MDCK-derived	1884:1895	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	10	98	theme	H3N2	1864:1867	arg1	strains					1875:1881	the H3N2 and B strains	1860:1881	the H3N2 and B strains	1860:1881	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	6	99	theme	different	1222:1230	arg1	glycans					1232:1238	50 different glycans	1219:1238	50 different glycans	1219:1238	A highly sensitive glycan assay was developed where 50 different glycans were identified, which was more than what has been reported previously, and their relative abundance was quantified.					
33937925	1	100	theme	critical	197:204	arg1	roles					206:210	critical roles	197:210	critical roles	197:210	N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines.					
33937925	2	101	theme	alternative	474:484	arg1	host					486:489	an important alternative host	461:489	an important alternative host	461:489	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	12	102	theme	trivalent	2092:2100	arg1	vaccines					2115:2122	trivalent seasonal flu vaccines	2092:2122	trivalent seasonal flu vaccines	2092:2122	• A systematic comparison of glycosylation on HAs used for trivalent seasonal flu vaccines was conducted.					
33937925	8	103	dep	studies	1567:1573	arg1	accounted					1647:1655	accounted	1647:1655	were accounted for in this study	1642:1673	High-mannose species account for the most abundant group of glycans, but at a lower level as compared to those reported in previous studies, presumably due to that lower abundance, complex structure glycans were accounted for in this study.					
33937925	12	104	theme	glycosylation	2062:2074	arg1	comparison					2048:2057	A systematic comparison	2035:2057	A systematic comparison of glycosylation on	2035:2077	• A systematic comparison of glycosylation on HAs used for trivalent seasonal flu vaccines was conducted.					
33937925	4	105	theme	glycosylation	942:954	arg1	sites					956:960	the 8, 12, and 11 potential glycosylation sites	914:960	the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively	914:1014	Of the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively, most were highly occupied.					
33937925	2	106	theme	predominant	350:360	arg1	host					373:376	the predominant production host	346:376	the predominant production host for influenza vaccines	346:399	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	2	106	theme	predominant	350:360	arg1	embryos					334:340	Chicken embryos	326:340	Chicken embryos	326:340	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	0	107	theme	glycosylation	23:35	arg1	Comparison					0:9	Comparison	0:9	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.	0:166	Comparison of N-linked glycosylation on hemagglutinins derived from chicken embryos and MDCK cells: a case of the production of a trivalent seasonal influenza vaccine.					
33937925	5	108	theme	B	1061:1061	arg1	strains					1063:1069	the H3N2 and B strains	1048:1069	strains	1063:1069	For the H3N2 and B strains, MDCK-derived HAs contained more sites being partially occupied (<95%) than embryo-derived HAs.					
33937925	2	109	theme	Madin-Darby	406:416	arg1	kidney					425:430	Madin-Darby canine kidney	406:430	Madin-Darby canine kidney (MDCK) cells	406:443	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	2	109	theme	Madin-Darby	406:416	arg1	MDCK					433:436	MDCK	433:436	MDCK	433:436	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	4	110	gly	glycosylation	942:954	arg2	12					921:922	12	921:922	12	921:922	Of the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively, most were highly occupied.					
33937925	4	110	gly	glycosylation	942:954	arg2	sites					956:960	the 8, 12, and 11 potential glycosylation sites	914:960	the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively	914:1014	Of the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively, most were highly occupied.					
33937925	4	110	gly	glycosylation	942:954	arg2	11					929:930	11	929:930	11	929:930	Of the 8, 12, and 11 potential glycosylation sites on the HAs of H1N1, H3N2, and B strains, respectively, most were highly occupied.					
33937925	3	111	theme	B/Brisbane/60/2008	876:893	arg1	###					905:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###	796:907	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	111	theme	B/Brisbane/60/2008	876:893	arg1	viruses					684:690	influenza viruses	674:690	influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###)	674:908	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	11	112	theme	weight	2025:2030	arg1	glycans					1997:2003	more glycans	1992:2003	more glycans of higher molecular weight	1992:2030	• MDCK-derived HAs contained more glycans of higher molecular weight.					
33937925	2	113	theme	influenza	382:390	arg1	vaccines					392:399	influenza vaccines	382:399	influenza vaccines	382:399	Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host.					
33937925	7	114	theme	more	1394:1397	arg1	glycans					1399:1405	more glycans	1394:1405	more glycans of higher molecular weight	1394:1432	In general, MDCK-derived HAs contain more glycans of higher molecular weight.					
33937925	9	115	theme	different	1680:1688	arg1	patterns					1704:1711	The different glycosylation patterns	1676:1711	The different glycosylation patterns between MDCK- and chicken embryo-derived HAs	1676:1756	The different glycosylation patterns between MDCK- and chicken embryo-derived HAs may help elucidate the role of glycosylation on the function of influenza vaccines.					
33937925	10	116	gly	glycosylation	1942:1954	arg2	sites					1956:1960	more partially (<95%) occupied glycosylation sites	1911:1960	more partially (<95%) occupied glycosylation sites	1911:1960	KEY POINTS: • For the H3N2 and B strains, MDCK-derived HAs contained more partially (<95%) occupied glycosylation sites.					
33937925	5	117	theme	H3N2	1052:1055	arg1	strains					1063:1069	the H3N2 and B strains	1048:1069	strains	1063:1069	For the H3N2 and B strains, MDCK-derived HAs contained more sites being partially occupied (<95%) than embryo-derived HAs.					
33937925	3	118	theme	glycans	634:640	arg1	occupancy					557:565	the occupancy	553:565	the occupancy of potential glycosylation sites	553:598	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
33937925	3	118	theme	glycans	634:640	arg1	distribution					608:619	the distribution	604:619	the distribution of different glycans	604:640	In this study, we compared glycosylation patterns, including the occupancy of potential glycosylation sites and the distribution of different glycans, on the HAs of three strains of influenza viruses for the production a trivalent seasonal flu vaccine for the 2015-2016 Northern Hemisphere season (i.e., A/California/7/2009 (H1N1) X179A, A/Switzerland/9715293/2013 (H3N2) NIB-88, and B/Brisbane/60/2008 NYMC BX-35###).					
32896313	4	0	theme	polymers	699:706	arg1	blocks					683:688	the building blocks	670:688	the building blocks of these polymers	670:706	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	3	1	theme	mechanical	515:524	arg1	support					526:532	mechanical support	515:532	mechanical support to plant structures	515:552	Wall components assemble and interact with one another to produce dynamic structures with many capabilities, including providing mechanical support to plant structures and determining plant cell shape and size.					
32896313	8	2	theme	xyloglucan-relevant	1428:1446	arg1	GTs					1448:1450	nine xyloglucan-relevant GTs	1423:1450	nine xyloglucan-relevant GTs from three different CAZy families	1423:1485	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	8	2	theme	xyloglucan-relevant	1428:1446	arg1	demonstration					1376:1388	a demonstration	1374:1388	a demonstration of the utility of this platform	1374:1420	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	6	3	attach	derived	1080:1086	arg2	Most					964:967	Most	964:967	Most	964:967	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	6	3	attach	derived	1080:1086	arg2	knowledge					976:984	the knowledge	972:984	the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway	972:1069	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	6	3	attach	derived	1080:1086	arg1	identification					1097:1110	identification	1097:1110	identification	1097:1110	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	6	3	attach	derived	1080:1086	arg1	analysis					1129:1136	carbohydrate analysis	1116:1136	carbohydrate analysis	1116:1136	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	5	4	from	weight	924:929	arg1	plants					956:961	plants	956:961	plants	956:961	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	8	5	theme	platform	1413:1420	arg1	utility					1397:1403	the utility	1393:1403	the utility of this platform	1393:1420	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	5	6	theme	walls	947:951	arg1	weight					924:929	the dry weight	916:929	the dry weight of primary cell walls in plants	916:961	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	4	7	theme	ineffective	731:741	arg1	methods					743:749	ineffective methods	731:749	ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses	731:843	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	6	8	theme	mutants	1150:1156	arg1	identification					1097:1110	identification	1097:1110	identification	1097:1110	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	6	8	theme	mutants	1150:1156	arg1	analysis					1129:1136	carbohydrate analysis	1116:1136	carbohydrate analysis	1116:1136	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	6	9	theme	catalytic	1197:1205	arg1	biosynthesis					1207:1218	the catalytic biosynthesis	1193:1218	the catalytic biosynthesis of xyloglucan	1193:1232	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	3	10	theme	plant	570:574	arg1	shape					581:585	plant cell shape	570:585	plant cell shape	570:585	Wall components assemble and interact with one another to produce dynamic structures with many capabilities, including providing mechanical support to plant structures and determining plant cell shape and size.					
32896313	5	11	theme	weight	924:929	arg1	%					911:911	up to 25%	903:911	up to 25% of the dry weight of primary cell walls in plants	903:961	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	5	11	theme	weight	924:929	arg1	weight					924:929	the dry weight	916:929	the dry weight of primary cell walls in plants	916:961	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	6	12	gly	carbohydrate	1116:1127	arg1	mutants					1150:1156	knockout mutants	1141:1156	knockout mutants	1141:1156	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	6	13	theme	little	1167:1172	arg1	information					1174:1184	little information	1167:1184	little information	1167:1184	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	4	14	theme	building	674:681	arg1	blocks					683:688	the building blocks	670:688	the building blocks of these polymers	670:706	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	7	15	theme	GTs	1329:1331	arg1	expression					1309:1318	the heterologous expression	1292:1318	the heterologous expression of plant GTs	1292:1331	In this chapter we describe methods for the heterologous expression of plant GTs using the HEK293 expression platform.					
32896313	6	16	theme	knockout	1141:1148	arg1	mutants					1150:1156	knockout mutants	1141:1156	knockout mutants	1141:1156	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	8	17	theme	different	1463:1471	arg1	families					1478:1485	three different CAZy families	1457:1485	three different CAZy families	1457:1485	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	1	18	theme	plant	195:199	arg1	walls					206:210	plant cell walls	195:210	plant cell walls	195:210	Much of the carbon captured by photosynthesis is converted into the polysaccharides that constitute plant cell walls.					
32896313	3	19	theme	Wall	386:389	arg1	components					391:400	Wall components	386:400	Wall components	386:400	Wall components assemble and interact with one another to produce dynamic structures with many capabilities, including providing mechanical support to plant structures and determining plant cell shape and size.					
32896313	5	20	from	walls	947:951	arg1	plants					956:961	plants	956:961	plants	956:961	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	4	21	theme	synthesis	657:665	arg1	knowledge					640:648	our knowledge	636:648	our knowledge of the synthesis of the building blocks of these polymers	636:706	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	2	22	theme	structural	329:338	arg1	proteins					340:347	structural proteins	329:347	structural proteins	329:347	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	1	23	theme	cell	201:204	arg1	walls					206:210	plant cell walls	195:210	plant cell walls	195:210	Much of the carbon captured by photosynthesis is converted into the polysaccharides that constitute plant cell walls.					
32896313	0	24	theme	Heterologous	0:11	arg1	expression					13:22	Heterologous expression	0:22	Heterologous expression of plant	0:31	Heterologous expression of plant glycosyltransferases for biochemistry and structural biology.					
32896313	5	25	theme	primary	934:940	arg1	walls					947:951	primary cell walls	934:951	primary cell walls in plants	934:961	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	6	26	theme	xyloglucan	1039:1048	arg1	pathway					1063:1069	the xyloglucan biosynthetic pathway	1035:1069	the xyloglucan biosynthetic pathway	1035:1069	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	0	27	theme	plant	27:31	arg1	expression					13:22	Heterologous expression	0:22	Heterologous expression of plant	0:31	Heterologous expression of plant glycosyltransferases for biochemistry and structural biology.					
32896313	8	28	theme	utility	1397:1403	arg1	GTs					1448:1450	nine xyloglucan-relevant GTs	1423:1450	nine xyloglucan-relevant GTs from three different CAZy families	1423:1485	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	8	28	theme	utility	1397:1403	arg1	demonstration					1376:1388	a demonstration	1374:1388	a demonstration of the utility of this platform	1374:1420	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	3	29	theme	cell	576:579	arg1	shape					581:585	plant cell shape	570:585	plant cell shape	570:585	Wall components assemble and interact with one another to produce dynamic structures with many capabilities, including providing mechanical support to plant structures and determining plant cell shape and size.					
32896313	7	30	theme	heterologous	1296:1307	arg1	expression					1309:1318	the heterologous expression	1292:1318	the heterologous expression of plant GTs	1292:1331	In this chapter we describe methods for the heterologous expression of plant GTs using the HEK293 expression platform.					
32896313	5	31	dep	25	909:910	arg1	to					906:907	to	906:907	to	906:907	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	3	32	with	structures	460:469	arg1	capabilities					481:492	many capabilities	476:492	many capabilities	476:492	Wall components assemble and interact with one another to produce dynamic structures with many capabilities, including providing mechanical support to plant structures and determining plant cell shape and size.					
32896313	5	33	theme	cell	942:945	arg1	walls					947:951	primary cell walls	934:951	primary cell walls in plants	934:961	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	2	34	theme	small	312:316	arg1	amounts					318:324	small amounts	312:324	small amounts of structural proteins	312:347	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	2	34	theme	small	312:316	arg1	proteins					340:347	structural proteins	329:347	structural proteins	329:347	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	3	35	theme	many	476:479	arg1	capabilities					481:492	many capabilities	476:492	many capabilities	476:492	Wall components assemble and interact with one another to produce dynamic structures with many capabilities, including providing mechanical support to plant structures and determining plant cell shape and size.					
32896313	8	36	theme	construct	1549:1557	arg1	optimization					1559:1570	construct optimization	1549:1570	construct optimization	1549:1570	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	5	37	theme	hemicellulosic	850:863	arg1	xyloglucan					881:890	xyloglucan	881:890	xyloglucan	881:890	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	5	37	theme	hemicellulosic	850:863	arg1	polysaccharide					865:878	The hemicellulosic polysaccharide	846:878	The hemicellulosic polysaccharide	846:878	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	3	38	dep	one	429:431	arg1	another					433:439	another	433:439	another	433:439	Wall components assemble and interact with one another to produce dynamic structures with many capabilities, including providing mechanical support to plant structures and determining plant cell shape and size.					
32896313	7	39	theme	plant	1323:1327	arg1	GTs					1329:1331	plant GTs	1323:1331	plant GTs	1323:1331	In this chapter we describe methods for the heterologous expression of plant GTs using the HEK293 expression platform.					
32896313	3	40	theme	dynamic	452:458	arg1	structures					460:469	dynamic structures	452:469	dynamic structures with many capabilities	452:492	Wall components assemble and interact with one another to produce dynamic structures with many capabilities, including providing mechanical support to plant structures and determining plant cell shape and size.					
32896313	6	41	theme	biosynthetic	1050:1061	arg1	pathway					1063:1069	the xyloglucan biosynthetic pathway	1035:1069	the xyloglucan biosynthetic pathway	1035:1069	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	4	42	theme	in	815:816	arg1	analyses					836:843	in vitro biochemical analyses	815:843	in vitro biochemical analyses	815:843	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	6	43	dep	identification	1097:1110	arg1	the					1093:1095	the	1093:1095	the	1093:1095	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	4	44	dep	in	815:816	arg1	vitro					818:822	vitro	818:822	vitro	818:822	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	4	45	from	gaps	628:631	arg1	knowledge					640:648	our knowledge	636:648	our knowledge of the synthesis of the building blocks of these polymers	636:706	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	6	46	theme	xyloglucan	1223:1232	arg1	biosynthesis					1207:1218	the catalytic biosynthesis	1193:1218	the catalytic biosynthesis of xyloglucan	1193:1232	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	2	47	theme	many	367:370	arg1	cases					372:376	many cases	367:376	many cases	367:376	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	8	48	theme	biochemical	1590:1600	arg1	characterization					1617:1632	biochemical and structural characterization	1590:1632	biochemical and structural characterization	1590:1632	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	8	49	theme	CAZy	1473:1476	arg1	families					1478:1485	three different CAZy families	1457:1485	three different CAZy families	1457:1485	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	4	50	theme	biochemical	824:834	arg1	analyses					836:843	in vitro biochemical analyses	815:843	in vitro biochemical analyses	815:843	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	6	51	theme	carbohydrate	1116:1127	arg1	analysis					1129:1136	carbohydrate analysis	1116:1136	carbohydrate analysis	1116:1136	Most of the knowledge about the glycosyltransferases (GTs) involved in the xyloglucan biosynthetic pathway has been derived from the identification and carbohydrate analysis of knockout mutants, lending little information on how the catalytic biosynthesis of xyloglucan occurs in planta.					
32896313	7	52	theme	HEK293	1343:1348	arg1	platform					1361:1368	the HEK293 expression platform	1339:1368	the HEK293 expression platform	1339:1368	In this chapter we describe methods for the heterologous expression of plant GTs using the HEK293 expression platform.					
32896313	2	53	theme	complex	219:225	arg1	macrostructures					227:241	These complex macrostructures	213:241	These complex macrostructures	213:241	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	4	54	theme	synthetic	793:801	arg1	enzymes					803:809	active synthetic enzymes	786:809	active synthetic enzymes for in vitro biochemical analyses	786:843	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	4	55	theme	blocks	683:688	arg1	synthesis					657:665	the synthesis	653:665	the synthesis of the building blocks of these polymers	653:706	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	5	56	theme	dry	920:922	arg1	weight					924:929	the dry weight	916:929	the dry weight of primary cell walls in plants	916:961	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	1	57	theme	carbon	107:112	arg1	Much					95:98	Much	95:98	Much	95:98	Much of the carbon captured by photosynthesis is converted into the polysaccharides that constitute plant cell walls.					
32896313	4	58	theme	major	622:626	arg1	gaps					628:631	major gaps	622:631	major gaps in our knowledge of the synthesis of the building blocks of these polymers	622:706	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	4	59	theme	active	786:791	arg1	enzymes					803:809	active synthetic enzymes	786:809	active synthetic enzymes for in vitro biochemical analyses	786:843	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	2	60	theme	proteins	340:347	arg1	amounts					318:324	small amounts	312:324	small amounts of structural proteins	312:347	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	2	60	theme	proteins	340:347	arg1	pectins					289:295	pectins	289:295	pectins	289:295	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	2	60	theme	proteins	340:347	arg1	cellulose					259:267	cellulose	259:267	cellulose	259:267	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	2	60	theme	proteins	340:347	arg1	cases					372:376	many cases	367:376	many cases	367:376	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	2	60	theme	proteins	340:347	arg1	proteins					340:347	structural proteins	329:347	structural proteins	329:347	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	2	60	theme	proteins	340:347	arg1	minerals					350:357	minerals	350:357	minerals	350:357	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	2	60	theme	proteins	340:347	arg1	hemicellulose					270:282	hemicellulose	270:282	hemicellulose	270:282	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	8	61	theme	structural	1606:1615	arg1	characterization					1617:1632	biochemical and structural characterization	1590:1632	biochemical and structural characterization	1590:1632	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	5	62	from	plants	956:961	arg1	weight					924:929	the dry weight	916:929	the dry weight of primary cell walls in plants	916:961	The hemicellulosic polysaccharide, xyloglucan, comprises up to 25% of the dry weight of primary cell walls in plants.					
32896313	2	63	dep	pectins	289:295	arg1	lignin					378:383	lignin	378:383	lignin	378:383	These complex macrostructures are composed of cellulose, hemicellulose, and pectins, together with small amounts of structural proteins, minerals, and in many cases lignin.					
32896313	0	64	theme	structural	75:84	arg1	biology					86:92	structural biology	75:92	structural biology	75:92	Heterologous expression of plant glycosyltransferases for biochemistry and structural biology.					
32896313	7	65	theme	expression	1350:1359	arg1	platform					1361:1368	the HEK293 expression platform	1339:1368	the HEK293 expression platform	1339:1368	In this chapter we describe methods for the heterologous expression of plant GTs using the HEK293 expression platform.					
32896313	4	66	theme	enzymes	803:809	arg1	purification					770:781	purification	770:781	purification	770:781	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	4	66	theme	enzymes	803:809	arg1	expression					755:764	expression	755:764	expression	755:764	Despite their abundance, major gaps in our knowledge of the synthesis of the building blocks of these polymers remain, largely due to ineffective methods for expression and purification of active synthetic enzymes for in vitro biochemical analyses.					
32896313	3	67	theme	plant	537:541	arg1	structures					543:552	plant structures	537:552	plant structures	537:552	Wall components assemble and interact with one another to produce dynamic structures with many capabilities, including providing mechanical support to plant structures and determining plant cell shape and size.					
32896313	8	68	from	families	1478:1485	arg1	GTs					1448:1450	nine xyloglucan-relevant GTs	1423:1450	nine xyloglucan-relevant GTs from three different CAZy families	1423:1485	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
32896313	8	68	from	families	1478:1485	arg1	demonstration					1376:1388	a demonstration	1374:1388	a demonstration of the utility of this platform	1374:1420	As a demonstration of the utility of this platform, nine xyloglucan-relevant GTs from three different CAZy families were evaluated, and methods for expression, purification, and construct optimization are described for biochemical and structural characterization.					
33361123	7	0	theme	potent	1559:1564	arg1	functions					1575:1583	potent effector functions	1559:1583	potent effector functions of immune cells	1559:1599	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	9	1	contain	had	1867:1869	arg2	structures					1887:1896	specific glycan structures	1871:1896	specific glycan structures	1871:1896	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	9	1	contain	had	1867:1869	arg1	Children					1854:1861	Children	1854:1861	Children who had specific glycan structures on the Fc portion of antibodies against HIV	1854:1940	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	4	2	theme	Pediatric	646:654	arg1	PNPs					672:675	PNPs	672:675	PNPs	672:675	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	4	2	theme	Pediatric	646:654	arg1	nonprogressors					656:669	Pediatric nonprogressors	646:669	Pediatric nonprogressors (PNPs)	646:676	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	10	3	from	knowledge	2130:2138	arg1	life					2182:2185	early life	2176:2185	early life	2176:2185	These findings complement our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies.					
33361123	3	4	theme	HIV-infected	579:590	arg1	children					592:599	HIV-infected children	579:599	HIV-infected children with progressive and nonprogressive disease	579:643	We therefore performed systems immunology, with immunoglobulin profiling, on HIV-infected children with progressive and nonprogressive disease.					
33361123	8	5	theme	Fc	1775:1776	arg1	functions					1787:1795	potent Fc effector functions	1768:1795	potent Fc effector functions	1768:1795	In this study, we show that nonprogressing HIV-infected children mounted antibody responses against HIV that were able to mediate potent Fc effector functions, which may contribute to the control of HIV replication.					
33361123	4	6	theme	killer	781:786	arg1	functions					807:815	potent Fc-mediated natural killer (NK)-cell effector functions	754:815	potent Fc-mediated natural killer (NK)-cell effector functions	754:815	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	10	7	theme	landscape	2163:2171	arg1	knowledge					2130:2138	our knowledge	2126:2138	our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies	2126:2250	These findings complement our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies.					
33361123	5	8	theme	gp120-specific	907:920	arg1	sialylation					936:946	gp120-specific IgG Fc glycan sialylation	907:946	gp120-specific IgG Fc glycan sialylation	907:946	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	7	9	theme	antibody	1518:1525	arg1	response					1527:1534	a broadly neutralizing antibody response	1495:1534	a broadly neutralizing antibody response	1495:1534	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	10	10	theme	distinct	2147:2154	arg1	landscape					2163:2171	the distinct immune landscape	2143:2171	the distinct immune landscape in early life	2143:2185	These findings complement our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies.					
33361123	5	11	theme	Fc	926:927	arg1	sialylation					936:946	gp120-specific IgG Fc glycan sialylation	907:946	gp120-specific IgG Fc glycan sialylation	907:946	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	6	12	theme	sialylation	1010:1020	arg1	levels					990:995	higher levels	983:995	higher levels of Fc glycan sialylation	983:1020	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	7	13	theme	HIV	1458:1460	arg1	vaccine					1462:1468	a prophylactic HIV vaccine	1443:1468	a prophylactic HIV vaccine	1443:1468	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	9	14	theme	HIV	1985:1987	arg1	variants					1989:1996	HIV variants	1985:1996	HIV variants	1985:1996	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	4	15	theme	distinct	685:692	arg1	profiles					709:716	distinct immunoglobulin profiles	685:716	distinct immunoglobulin profiles	685:716	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	6	16	theme	Fc	1000:1001	arg1	sialylation					1010:1020	Fc glycan sialylation	1000:1020	Fc glycan sialylation	1000:1020	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	10	17	theme	early	2176:2180	arg1	life					2182:2185	early life	2176:2185	early life	2176:2185	These findings complement our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies.					
33361123	0	18	theme	HIV	154:156	arg1	Infection					158:166	HIV Infection	154:166	HIV Infection	154:166	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	9	19	theme	specific	1871:1878	arg1	structures					1887:1896	specific glycan structures	1871:1896	specific glycan structures	1871:1896	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	6	20	dep	protect	1263:1269	arg1	need					1318:1321	need	1318:1321	will need to induce immunity by the time of sexual debut and hence requires immunization during childhood	1313:1417	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	6	21	theme	sexual	1357:1362	arg1	debut					1364:1368	sexual debut	1357:1368	sexual debut	1357:1368	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	0	22	from	Responses	127:135	arg1	Children					140:147	Children	140:147	Children with HIV Infection	140:166	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	2	23	theme	neutralizing	285:296	arg1	responses					314:322	neutralizing antibody (bnAb) responses	285:322	neutralizing antibody (bnAb) responses	285:322	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	8	24	theme	replication	1841:1851	arg1	control					1826:1832	the control	1822:1832	the control of HIV replication	1822:1851	In this study, we show that nonprogressing HIV-infected children mounted antibody responses against HIV that were able to mediate potent Fc effector functions, which may contribute to the control of HIV replication.					
33361123	9	25	theme	role	2033:2036	arg1	evidence					2009:2016	evidence	2009:2016	evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV	2009:2097	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	0	26	with	Children	140:147	arg1	Infection					158:166	HIV Infection	154:166	HIV Infection	154:166	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	0	27	theme	Disease	70:76	arg1	Nonprogression					78:91	Disease Nonprogression	70:91	Disease Nonprogression	70:91	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	5	28	theme	sialylation	936:946	arg1	levels					897:902	high levels	892:902	high levels of gp120-specific IgG Fc glycan sialylation	892:946	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	8	29	theme	antibody	1711:1718	arg1	responses					1720:1728	antibody responses	1711:1728	antibody responses	1711:1728	In this study, we show that nonprogressing HIV-infected children mounted antibody responses against HIV that were able to mediate potent Fc effector functions, which may contribute to the control of HIV replication.					
33361123	9	30	theme	Fc	2041:2042	arg1	glycovariation					2044:2057	Fc glycovariation	2041:2057	Fc glycovariation	2041:2057	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	4	31	theme	Fc-mediated	761:771	arg1	killer					781:786	Fc-mediated natural killer	761:786	potent Fc-mediated natural killer (NK)-cell effector functions	754:815	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	4	31	theme	Fc-mediated	761:771	arg1	NK					789:790	NK	789:790	NK	789:790	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	1	32	theme	HIV	184:186	arg1	vaccine					188:194	A prophylactic HIV vaccine	169:194	A prophylactic HIV vaccine	169:194	A prophylactic HIV vaccine would ideally induce protective immunity prior to sexual debut.					
33361123	6	33	theme	increased	1043:1051	arg1	breadth					1058:1064	increased bnAb breadth	1043:1064	increased bnAb breadth	1043:1064	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	7	34	theme	Current	1420:1426	arg1	strategies					1428:1437	Current strategies	1420:1437	Current strategies for a prophylactic HIV vaccine	1420:1468	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	0	35	theme	Neutralizing	105:116	arg1	Responses					127:135	Broadly Neutralizing Antibody Responses	97:135	Broadly Neutralizing Antibody Responses	97:135	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	7	36	theme	Fc	1627:1628	arg1	region					1630:1635	the constant antibody Fc region	1605:1635	the constant antibody Fc region	1605:1635	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	2	37	theme	functions	468:476	arg1	mechanisms					411:420	the underlying mechanisms	396:420	the underlying mechanisms	396:420	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	2	37	theme	functions	468:476	arg1	role					439:442	the potential role	425:442	the potential role of Fc-mediated effector functions in disease progression	425:499	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	9	38	theme	bnAbs	2081:2085	arg1	development					2066:2076	the development	2062:2076	the development of bnAbs against HIV	2062:2097	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	4	39	theme	-cell	792:796	arg1	functions					807:815	potent Fc-mediated natural killer (NK)-cell effector functions	754:815	potent Fc-mediated natural killer (NK)-cell effector functions	754:815	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	0	40	theme	Distinct	0:7	arg1	Patterns					41:48	Distinct Immunoglobulin Fc Glycosylation Patterns	0:48	Distinct Immunoglobulin Fc Glycosylation Patterns	0:48	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	9	41	from	role	2033:2036	arg1	development					2066:2076	the development	2062:2076	the development of bnAbs against HIV	2062:2097	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	2	42	theme	Fc-mediated	447:457	arg1	functions					468:476	Fc-mediated effector functions	447:476	Fc-mediated effector functions	447:476	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	0	43	theme	Fc	24:25	arg1	Patterns					41:48	Distinct Immunoglobulin Fc Glycosylation Patterns	0:48	Distinct Immunoglobulin Fc Glycosylation Patterns	0:48	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	7	44	theme	cells	1595:1599	arg1	functions					1575:1583	potent effector functions	1559:1583	potent effector functions of immune cells	1559:1599	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	6	45	theme	first	1081:1085	arg1	evidence					1087:1094	the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood	1077:1417	the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood	1077:1417	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	2	46	theme	disease	481:487	arg1	progression					489:499	disease progression	481:499	disease progression	481:499	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	2	47	from	mechanisms	411:420	arg1	progression					489:499	disease progression	481:499	disease progression	481:499	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	10	48	from	life	2182:2185	arg1	knowledge					2130:2138	our knowledge	2126:2138	our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies	2126:2250	These findings complement our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies.					
33361123	8	49	theme	HIV	1837:1839	arg1	replication					1841:1851	HIV replication	1837:1851	HIV replication	1837:1851	In this study, we show that nonprogressing HIV-infected children mounted antibody responses against HIV that were able to mediate potent Fc effector functions, which may contribute to the control of HIV replication.					
33361123	1	50	theme	sexual	246:251	arg1	debut					253:257	sexual debut	246:257	sexual debut	246:257	A prophylactic HIV vaccine would ideally induce protective immunity prior to sexual debut.					
33361123	7	51	theme	effector	1566:1573	arg1	functions					1575:1583	potent effector functions	1559:1583	potent effector functions of immune cells	1559:1599	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	9	52	theme	antibodies	1919:1928	arg1	portion					1908:1914	the Fc portion	1901:1914	the Fc portion of antibodies against HIV	1901:1940	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	10	53	theme	immune	2156:2161	arg1	landscape					2163:2171	the distinct immune landscape	2143:2171	the distinct immune landscape in early life	2143:2185	These findings complement our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies.					
33361123	9	54	from	evidence	2009:2016	arg1	development					2066:2076	the development	2062:2076	the development of bnAbs against HIV	2062:2097	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	6	55	from	maturation	1135:1144	arg1	children					1176:1183	children	1176:1183	children	1176:1183	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	6	55	from	maturation	1135:1144	arg1	mechanism					1188:1196	a mechanism	1186:1196	a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood	1186:1417	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	2	56	from	role	439:442	arg1	progression					489:499	disease progression	481:499	disease progression	481:499	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	6	57	theme	HIV-specific	1149:1160	arg1	antibodies					1162:1171	HIV-specific antibodies	1149:1171	HIV-specific antibodies	1149:1171	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	9	58	from	development	2066:2076	arg1	evidence					2009:2016	evidence	2009:2016	evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV	2009:2097	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	10	59	theme	strategies	2241:2250	arg1	development					2218:2228	the development	2214:2228	the development of vaccine strategies	2214:2250	These findings complement our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies.					
33361123	8	60	theme	HIV-infected	1681:1692	arg1	children					1694:1701	nonprogressing HIV-infected children	1666:1701	nonprogressing HIV-infected children	1666:1701	In this study, we show that nonprogressing HIV-infected children mounted antibody responses against HIV that were able to mediate potent Fc effector functions, which may contribute to the control of HIV replication.					
33361123	3	61	theme	progressive	606:616	arg1	disease					637:643	progressive and nonprogressive disease	606:643	progressive and nonprogressive disease	606:643	We therefore performed systems immunology, with immunoglobulin profiling, on HIV-infected children with progressive and nonprogressive disease.					
33361123	7	62	theme	response	1527:1534	arg1	recruitment					1544:1554	the recruitment	1540:1554	the recruitment of potent effector functions of immune cells	1540:1599	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	7	62	theme	response	1527:1534	arg1	induction					1482:1490	the induction	1478:1490	the induction of a broadly neutralizing antibody response	1478:1534	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	6	63	theme	vaccination	1226:1236	arg1	strategies.IMPORTANCE					1238:1258	vaccination strategies.IMPORTANCE	1226:1258	vaccination strategies.IMPORTANCE	1226:1258	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	2	64	theme	bnAb	308:311	arg1	responses					314:322	neutralizing antibody (bnAb) responses	285:322	neutralizing antibody (bnAb) responses	285:322	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	8	65	theme	potent	1768:1773	arg1	functions					1787:1795	potent Fc effector functions	1768:1795	potent Fc effector functions	1768:1795	In this study, we show that nonprogressing HIV-infected children mounted antibody responses against HIV that were able to mediate potent Fc effector functions, which may contribute to the control of HIV replication.					
33361123	7	66	theme	neutralizing	1505:1516	arg1	response					1527:1534	a broadly neutralizing antibody response	1495:1534	a broadly neutralizing antibody response	1495:1534	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	9	67	theme	broader	1968:1974	arg1	range					1976:1980	a broader range	1966:1980	a broader range of HIV variants	1966:1996	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	2	68	theme	higher	338:343	arg1	frequencies					345:355	higher frequencies	338:355	higher frequencies	338:355	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	8	69	theme	effector	1778:1785	arg1	functions					1787:1795	potent Fc effector functions	1768:1795	potent Fc effector functions	1768:1795	In this study, we show that nonprogressing HIV-infected children mounted antibody responses against HIV that were able to mediate potent Fc effector functions, which may contribute to the control of HIV replication.					
33361123	5	70	theme	IgG	922:924	arg1	sialylation					936:946	gp120-specific IgG Fc glycan sialylation	907:946	gp120-specific IgG Fc glycan sialylation	907:946	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	4	71	theme	increased	726:734	arg1	ability					736:742	an increased ability	723:742	an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions	723:815	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	5	72	theme	children	876:883	arg1	children					876:883	children	876:883	children	876:883	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	5	72	theme	children	876:883	arg1	groups					866:871	both groups	861:871	both groups of children	861:883	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	6	73	theme	glycan	1003:1008	arg1	sialylation					1010:1020	Fc glycan sialylation	1000:1020	Fc glycan sialylation	1000:1020	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	9	74	theme	variants	1989:1996	arg1	range					1976:1980	a broader range	1966:1980	a broader range of HIV variants	1966:1996	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	5	75	theme	high	892:895	arg1	levels					897:902	high levels	892:902	high levels of gp120-specific IgG Fc glycan sialylation	892:946	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	0	76	from	Nonprogression	78:91	arg1	Children					140:147	Children	140:147	Children with HIV Infection	140:166	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	7	77	theme	prophylactic	1445:1456	arg1	vaccine					1462:1468	a prophylactic HIV vaccine	1443:1468	a prophylactic HIV vaccine	1443:1468	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	4	78	theme	immunoglobulin	694:707	arg1	profiles					709:716	distinct immunoglobulin profiles	685:716	distinct immunoglobulin profiles	685:716	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	6	79	theme	debut	1364:1368	arg1	time					1349:1352	the time	1345:1352	the time of sexual debut	1345:1368	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	3	80	theme	nonprogressive	622:635	arg1	disease					637:643	progressive and nonprogressive disease	606:643	progressive and nonprogressive disease	606:643	We therefore performed systems immunology, with immunoglobulin profiling, on HIV-infected children with progressive and nonprogressive disease.					
33361123	5	81	theme	bulk	960:963	arg1	IgG					965:967	bulk IgG	960:967	bulk IgG	960:967	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	6	82	theme	higher	983:988	arg1	levels					990:995	higher levels	983:995	higher levels of Fc glycan sialylation	983:1020	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	4	83	theme	natural	773:779	arg1	killer					781:786	Fc-mediated natural killer	761:786	potent Fc-mediated natural killer (NK)-cell effector functions	754:815	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	4	83	theme	natural	773:779	arg1	NK					789:790	NK	789:790	NK	789:790	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	2	84	theme	antibody	298:305	arg1	responses					314:322	neutralizing antibody (bnAb) responses	285:322	neutralizing antibody (bnAb) responses	285:322	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	6	85	theme	future	1271:1276	arg1	generations					1278:1288	future generations	1271:1288	future generations	1271:1288	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	9	86	theme	potential	2023:2031	arg1	role					2033:2036	a potential role	2021:2036	a potential role of Fc glycovariation in the development of bnAbs against HIV	2021:2097	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	1	87	theme	protective	217:226	arg1	immunity					228:235	protective immunity	217:235	protective immunity	217:235	A prophylactic HIV vaccine would ideally induce protective immunity prior to sexual debut.					
33361123	5	88	theme	glycan	929:934	arg1	sialylation					936:946	gp120-specific IgG Fc glycan sialylation	907:946	gp120-specific IgG Fc glycan sialylation	907:946	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	0	89	theme	Broadly	97:103	arg1	Responses					127:135	Broadly Neutralizing Antibody Responses	97:135	Broadly Neutralizing Antibody Responses	97:135	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	8	90	dep	mounted	1703:1709	arg1	contribute					1808:1817	contribute	1808:1817	may contribute to the control of HIV replication	1804:1851	In this study, we show that nonprogressing HIV-infected children mounted antibody responses against HIV that were able to mediate potent Fc effector functions, which may contribute to the control of HIV replication.					
33361123	1	91	theme	prophylactic	171:182	arg1	vaccine					188:194	A prophylactic HIV vaccine	169:194	A prophylactic HIV vaccine	169:194	A prophylactic HIV vaccine would ideally induce protective immunity prior to sexual debut.					
33361123	6	92	theme	bnAb	1053:1056	arg1	breadth					1058:1064	increased bnAb breadth	1043:1064	increased bnAb breadth	1043:1064	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	9	93	theme	glycovariation	2044:2057	arg1	role					2033:2036	a potential role	2021:2036	a potential role of Fc glycovariation in the development of bnAbs against HIV	2021:2097	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	4	94	theme	potent	754:759	arg1	functions					807:815	potent Fc-mediated natural killer (NK)-cell effector functions	754:815	potent Fc-mediated natural killer (NK)-cell effector functions	754:815	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	0	95	theme	Antibody	118:125	arg1	Responses					127:135	Broadly Neutralizing Antibody Responses	97:135	Broadly Neutralizing Antibody Responses	97:135	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	5	96	from	reports	842:848	arg1	adults					853:858	adults	853:858	adults	853:858	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	7	97	theme	antibody	1618:1625	arg1	region					1630:1635	the constant antibody Fc region	1605:1635	the constant antibody Fc region	1605:1635	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	10	98	theme	vaccine	2233:2239	arg1	strategies					2241:2250	vaccine strategies	2233:2250	vaccine strategies	2233:2250	These findings complement our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies.					
33361123	6	99	theme	affinity	1126:1133	arg1	maturation					1135:1144	affinity maturation	1126:1144	affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood	1126:1417	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	2	100	theme	underlying	400:409	arg1	mechanisms					411:420	the underlying mechanisms	396:420	the underlying mechanisms	396:420	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	0	101	theme	Immunoglobulin	9:22	arg1	Patterns					41:48	Distinct Immunoglobulin Fc Glycosylation Patterns	0:48	Distinct Immunoglobulin Fc Glycosylation Patterns	0:48	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	2	102	theme	effector	459:466	arg1	functions					468:476	Fc-mediated effector functions	447:476	Fc-mediated effector functions	447:476	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	0	103	theme	Glycosylation	27:39	arg1	Patterns					41:48	Distinct Immunoglobulin Fc Glycosylation Patterns	0:48	Distinct Immunoglobulin Fc Glycosylation Patterns	0:48	Distinct Immunoglobulin Fc Glycosylation Patterns Are Associated with Disease Nonprogression and Broadly Neutralizing Antibody Responses in Children with HIV Infection.					
33361123	6	104	theme	Fc	1101:1102	arg1	sialylation					1104:1114	Fc sialylation	1101:1114	Fc sialylation	1101:1114	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	9	105	theme	glycan	1880:1885	arg1	structures					1887:1896	specific glycan structures	1871:1896	specific glycan structures	1871:1896	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	4	106	theme	effector	798:805	arg1	functions					807:815	potent Fc-mediated natural killer (NK)-cell effector functions	754:815	potent Fc-mediated natural killer (NK)-cell effector functions	754:815	Pediatric nonprogressors (PNPs) showed distinct immunoglobulin profiles with an increased ability to elicit potent Fc-mediated natural killer (NK)-cell effector functions.					
33361123	2	107	theme	potential	429:437	arg1	role					439:442	the potential role	425:442	the potential role of Fc-mediated effector functions in disease progression	425:499	Children develop broadly neutralizing antibody (bnAb) responses faster and at higher frequencies than adults, but little is known about the underlying mechanisms or the potential role of Fc-mediated effector functions in disease progression.					
33361123	5	108	theme	previous	833:840	arg1	reports					842:848	previous reports	833:848	previous reports in adults	833:858	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	7	109	theme	constant	1609:1616	arg1	region					1630:1635	the constant antibody Fc region	1605:1635	the constant antibody Fc region	1605:1635	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	9	110	theme	Fc	1905:1906	arg1	portion					1908:1914	the Fc portion	1901:1914	the Fc portion of antibodies against HIV	1901:1940	Children who had specific glycan structures on the Fc portion of antibodies against HIV were able to neutralize a broader range of HIV variants, providing evidence of a potential role of Fc glycovariation in the development of bnAbs against HIV.					
33361123	10	111	from	landscape	2163:2171	arg1	life					2182:2185	early life	2176:2185	early life	2176:2185	These findings complement our knowledge of the distinct immune landscape in early life that could be exploited in the development of vaccine strategies.					
33361123	3	112	theme	systems	525:531	arg1	immunology					533:542	systems immunology	525:542	systems immunology	525:542	We therefore performed systems immunology, with immunoglobulin profiling, on HIV-infected children with progressive and nonprogressive disease.					
33361123	7	113	theme	immune	1588:1593	arg1	cells					1595:1599	immune cells	1588:1599	immune cells	1588:1599	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	3	114	theme	immunoglobulin	550:563	arg1	profiling					565:573	immunoglobulin profiling	550:573	immunoglobulin profiling	550:573	We therefore performed systems immunology, with immunoglobulin profiling, on HIV-infected children with progressive and nonprogressive disease.					
33361123	7	115	theme	functions	1575:1583	arg1	recruitment					1544:1554	the recruitment	1540:1554	the recruitment of potent effector functions of immune cells	1540:1599	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	7	115	theme	functions	1575:1583	arg1	induction					1482:1490	the induction	1478:1490	the induction of a broadly neutralizing antibody response	1478:1534	Current strategies for a prophylactic HIV vaccine include the induction of a broadly neutralizing antibody response and the recruitment of potent effector functions of immune cells via the constant antibody Fc region.					
33361123	6	116	theme	antibodies	1162:1171	arg1	maturation					1135:1144	affinity maturation	1126:1144	affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood	1126:1417	Importantly, higher levels of Fc glycan sialylation were associated with increased bnAb breadth, providing the first evidence that Fc sialylation may drive affinity maturation of HIV-specific antibodies in children, a mechanism that could be exploited for vaccination strategies.IMPORTANCE To protect future generations against HIV, a vaccine will need to induce immunity by the time of sexual debut and hence requires immunization during childhood.					
33361123	8	117	theme	nonprogressing	1666:1679	arg1	children					1694:1701	nonprogressing HIV-infected children	1666:1701	nonprogressing HIV-infected children	1666:1701	In this study, we show that nonprogressing HIV-infected children mounted antibody responses against HIV that were able to mediate potent Fc effector functions, which may contribute to the control of HIV replication.					
33361123	5	118	dep	showed	885:890	arg1	compared					948:955	compared	948:955	showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG	885:967	In contrast to previous reports in adults, both groups of children showed high levels of gp120-specific IgG Fc glycan sialylation compared to bulk IgG.					
33361123	3	119	with	children	592:599	arg1	disease					637:643	progressive and nonprogressive disease	606:643	progressive and nonprogressive disease	606:643	We therefore performed systems immunology, with immunoglobulin profiling, on HIV-infected children with progressive and nonprogressive disease.					
32568201	0	0	theme	Discovering	72:82	arg1	Glycosylation					101:113	Discovering Isoform-Specific Glycosylation	72:113	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.	0:148	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.					
32568201	13	1	theme	apoE	1757:1760	arg1	metabolism					1762:1771	brain apoE metabolism	1751:1771	brain apoE metabolism	1751:1771	Assessing apoE isoform-specific glycosylation by MSIA may help clarify brain apoE metabolism and AD risk.					
32568201	1	2	theme	disease	239:245	arg1	pathology					252:260	Alzheimer's disease (AD) pathology	227:260	Alzheimer's disease (AD) pathology	227:260	BACKGROUND The mechanisms of how APOEɛ4 allele (APOE4) increases the risk of Alzheimer's disease (AD) pathology have not been fully elucidated.					
32568201	7	3	theme	same	1131:1134	arg1	type					1136:1139	the same type	1127:1139	the same type	1127:1139	For all individuals, a single O-linked glycan was observed in plasma, while two glycans (of the same type) per apoE were observed in CSF.					
32568201	7	4	theme	single	1058:1063	arg1	glycan					1074:1079	a single O-linked glycan	1056:1079	a single O-linked glycan	1056:1079	For all individuals, a single O-linked glycan was observed in plasma, while two glycans (of the same type) per apoE were observed in CSF.					
32568201	7	5	located	observed	1085:1092	arg1	plasma					1097:1102	plasma	1097:1102	plasma	1097:1102	For all individuals, a single O-linked glycan was observed in plasma, while two glycans (of the same type) per apoE were observed in CSF.					
32568201	7	5	located	observed	1085:1092	arg2	glycan					1074:1079	a single O-linked glycan	1056:1079	a single O-linked glycan	1056:1079	For all individuals, a single O-linked glycan was observed in plasma, while two glycans (of the same type) per apoE were observed in CSF.					
32568201	3	6	theme	specific	466:473	arg1	patterns					489:496	their specific glycosylation patterns	460:496	their specific glycosylation patterns	460:496	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	10	7	from	percentage	1392:1401	arg1	CSF					1433:1435	CSF	1433:1435	CSF	1433:1435	The difference in the percentage of secondary glycosylation in CSF was significantly greater in apoE4 compared to the other isoforms.					
32568201	3	8	from	impact	381:386	arg1	abundance					421:429	the relative abundance	408:429	the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma	408:514	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	10	9	from	difference	1374:1383	arg1	percentage					1392:1401	the percentage	1388:1401	the percentage of secondary glycosylation in CSF	1388:1435	The difference in the percentage of secondary glycosylation in CSF was significantly greater in apoE4 compared to the other isoforms.					
32568201	0	10	theme	Isoform-Specific	84:99	arg1	Glycosylation					101:113	Discovering Isoform-Specific Glycosylation	72:113	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.	0:148	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.					
32568201	11	11	theme	apoE	1528:1531	arg1	assay					1533:1537	The new MSIA apoE assay	1515:1537	The new MSIA apoE assay	1515:1537	CONCLUSION The new MSIA apoE assay robustly distinguishes among apoE isoforms and glycoforms in plasma and CSF.					
32568201	9	12	located	observed	1333:1340	arg2	trend					1295:1299	a trend	1293:1299	a trend of decreasing glycosylation	1293:1327	In plasma and CSF, a trend of decreasing glycosylation was observed from apoE2 > apoE3 > apoE4.					
32568201	9	12	located	observed	1333:1340	arg1	plasma					1277:1282	plasma	1277:1282	plasma	1277:1282	In plasma and CSF, a trend of decreasing glycosylation was observed from apoE2 > apoE3 > apoE4.					
32568201	9	12	located	observed	1333:1340	arg1	CSF					1288:1290	CSF	1288:1290	CSF	1288:1290	In plasma and CSF, a trend of decreasing glycosylation was observed from apoE2 > apoE3 > apoE4.					
32568201	12	13	theme	predominant	1629:1639	arg1	isoform					1641:1647	the predominant isoform	1625:1647	the predominant isoform	1625:1647	ApoE4 is the predominant isoform and least glycosylated in CSF.					
32568201	12	13	theme	predominant	1629:1639	arg1	ApoE4					1616:1620	ApoE4	1616:1620	ApoE4	1616:1620	ApoE4 is the predominant isoform and least glycosylated in CSF.					
32568201	3	14	theme	protein	439:445	arg1	isoforms					447:454	apoE protein isoforms	434:454	apoE protein isoforms	434:454	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	9	15	theme	>	1353:1353	arg1	apoE4					1363:1367	apoE2 > apoE3 > apoE4	1347:1367	apoE2 > apoE3 > apoE4	1347:1367	In plasma and CSF, a trend of decreasing glycosylation was observed from apoE2 > apoE3 > apoE4.					
32568201	5	16	with	treatment	870:878	arg1	sialidase					885:893	sialidase	885:893	sialidase	885:893	The glycan structures were further confirmed after treatment with sialidase.					
32568201	10	17	from	CSF	1433:1435	arg1	percentage					1392:1401	the percentage	1388:1401	the percentage of secondary glycosylation in CSF	1388:1435	The difference in the percentage of secondary glycosylation in CSF was significantly greater in apoE4 compared to the other isoforms.					
32568201	6	18	dep	lower	1002:1006	arg1	compared					1018:1025	compared	1018:1025	compared to CSF	1018:1032	RESULTS In heterozygous individuals, the apoE3/E2, E4/E2, and E4/E3 isoform ratios were all significantly lower in plasma compared to CSF.					
32568201	10	19	gly	glycosylation	1416:1428	arg1	CSF					1433:1435	CSF	1433:1435	CSF	1433:1435	The difference in the percentage of secondary glycosylation in CSF was significantly greater in apoE4 compared to the other isoforms.					
32568201	3	20	theme	genotype	396:403	arg1	impact					381:386	the impact	377:386	the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma	377:514	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	10	21	theme	glycosylation	1416:1428	arg1	percentage					1392:1401	the percentage	1388:1401	the percentage of secondary glycosylation in CSF	1388:1435	The difference in the percentage of secondary glycosylation in CSF was significantly greater in apoE4 compared to the other isoforms.					
32568201	11	22	theme	new	1519:1521	arg1	assay					1533:1537	The new MSIA apoE assay	1515:1537	The new MSIA apoE assay	1515:1537	CONCLUSION The new MSIA apoE assay robustly distinguishes among apoE isoforms and glycoforms in plasma and CSF.					
32568201	10	23	theme	other	1488:1492	arg1	isoforms					1494:1501	the other isoforms	1484:1501	the other isoforms	1484:1501	The difference in the percentage of secondary glycosylation in CSF was significantly greater in apoE4 compared to the other isoforms.					
32568201	13	24	theme	isoform-specific	1695:1710	arg1	glycosylation					1712:1724	apoE isoform-specific glycosylation	1690:1724	apoE isoform-specific glycosylation	1690:1724	Assessing apoE isoform-specific glycosylation by MSIA may help clarify brain apoE metabolism and AD risk.					
32568201	1	25	theme	APOEɛ4	183:188	arg1	allele					190:195	APOEɛ4 allele	183:195	APOEɛ4 allele (APOE4)	183:203	BACKGROUND The mechanisms of how APOEɛ4 allele (APOE4) increases the risk of Alzheimer's disease (AD) pathology have not been fully elucidated.					
32568201	1	25	theme	APOEɛ4	183:188	arg1	APOE4					198:202	APOE4	198:202	APOE4	198:202	BACKGROUND The mechanisms of how APOEɛ4 allele (APOE4) increases the risk of Alzheimer's disease (AD) pathology have not been fully elucidated.					
32568201	11	26	theme	MSIA	1523:1526	arg1	assay					1533:1537	The new MSIA apoE assay	1515:1537	The new MSIA apoE assay	1515:1537	CONCLUSION The new MSIA apoE assay robustly distinguishes among apoE isoforms and glycoforms in plasma and CSF.					
32568201	10	27	theme	secondary	1406:1414	arg1	glycosylation					1416:1428	secondary glycosylation	1406:1428	secondary glycosylation in CSF	1406:1435	The difference in the percentage of secondary glycosylation in CSF was significantly greater in apoE4 compared to the other isoforms.					
32568201	4	28	theme	older	707:711	arg1	individuals					713:723	non-demented older individuals	694:723	non-demented older individuals (n = 22)	694:732	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	4	28	theme	older	707:711	arg1	n = 22					726:731	n = 22	726:731	n = 22	726:731	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	9	29	theme	decreasing	1304:1313	arg1	glycosylation					1315:1327	decreasing glycosylation	1304:1327	decreasing glycosylation	1304:1327	In plasma and CSF, a trend of decreasing glycosylation was observed from apoE2 > apoE3 > apoE4.					
32568201	0	30	theme	Simple	0:5	arg1	Assay					16:20	Simple and Fast Assay	0:20	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.	0:148	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.					
32568201	1	31	theme	pathology	252:260	arg1	risk					219:222	the risk	215:222	the risk of Alzheimer's disease (AD) pathology	215:260	BACKGROUND The mechanisms of how APOEɛ4 allele (APOE4) increases the risk of Alzheimer's disease (AD) pathology have not been fully elucidated.					
32568201	2	32	gly	glycosylated	340:351	arg1	CSF					318:320	CSF	318:320	CSF	318:320	In cerebrospinal fluid (CSF), apoE is heavily glycosylated.					
32568201	2	32	gly	glycosylated	340:351	arg2	apoE					324:327	apoE	324:327	apoE	324:327	In cerebrospinal fluid (CSF), apoE is heavily glycosylated.					
32568201	2	32	gly	glycosylated	340:351	arg1	apoE					324:327	apoE	324:327	apoE	324:327	In cerebrospinal fluid (CSF), apoE is heavily glycosylated.					
32568201	2	32	gly	glycosylated	340:351	arg1	fluid					311:315	cerebrospinal fluid	297:315	cerebrospinal fluid (CSF)	297:321	In cerebrospinal fluid (CSF), apoE is heavily glycosylated.					
32568201	3	33	theme	developed	528:536	arg1	assay					576:580	a newly developed mass spectrometric immunoassay (MSIA) assay	520:580	a newly developed mass spectrometric immunoassay (MSIA) assay	520:580	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	13	34	theme	apoE	1690:1693	arg1	glycosylation					1712:1724	apoE isoform-specific glycosylation	1690:1724	apoE isoform-specific glycosylation	1690:1724	Assessing apoE isoform-specific glycosylation by MSIA may help clarify brain apoE metabolism and AD risk.					
32568201	3	35	theme	APOE	391:394	arg1	genotype					396:403	APOE genotype	391:403	APOE genotype	391:403	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	4	36	theme	non-demented	694:705	arg1	individuals					713:723	non-demented older individuals	694:723	non-demented older individuals (n = 22)	694:732	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	4	36	theme	non-demented	694:705	arg1	n = 22					726:731	n = 22	726:731	n = 22	726:731	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	0	37	theme	Cerebrospinal	129:141	arg1	Fluid					143:147	Cerebrospinal Fluid	129:147	Cerebrospinal Fluid	129:147	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.					
32568201	4	38	dep	METHODS	583:589	arg1	glycosylation					597:609	Total glycosylation	591:609	Total glycosylation	591:609	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	4	38	dep	METHODS	583:589	arg1	glycosylation					632:644	isoform-specific glycosylation	615:644	isoform-specific glycosylation	615:644	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	4	38	dep	METHODS	583:589	arg1	METHODS					583:589	METHODS	583:589	METHODS Total glycosylation and isoform-specific glycosylation	583:644	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	1	39	dep	BACKGROUND	150:159	arg1	elucidated					282:291	elucidated	282:291	have not been fully elucidated	262:291	BACKGROUND The mechanisms of how APOEɛ4 allele (APOE4) increases the risk of Alzheimer's disease (AD) pathology have not been fully elucidated.					
32568201	7	40	link	O-linked	1065:1072	arg1	glycan					1074:1079	a single O-linked glycan	1056:1079	a single O-linked glycan	1056:1079	For all individuals, a single O-linked glycan was observed in plasma, while two glycans (of the same type) per apoE were observed in CSF.					
32568201	4	41	theme	Total	591:595	arg1	glycosylation					597:609	Total glycosylation	591:609	Total glycosylation	591:609	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	4	41	theme	Total	591:595	arg1	METHODS					583:589	METHODS	583:589	METHODS Total glycosylation and isoform-specific glycosylation	583:644	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	0	42	theme	Fast	11:14	arg1	Assay					16:20	Simple and Fast Assay	0:20	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.	0:148	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.					
32568201	11	43	dep	CONCLUSION	1504:1513	arg1	distinguishes					1548:1560	distinguishes	1548:1560	distinguishes among apoE isoforms and glycoforms in plasma and CSF	1548:1613	CONCLUSION The new MSIA apoE assay robustly distinguishes among apoE isoforms and glycoforms in plasma and CSF.					
32568201	9	44	theme	>	1361:1361	arg1	apoE4					1363:1367	apoE2 > apoE3 > apoE4	1347:1367	apoE2 > apoE3 > apoE4	1347:1367	In plasma and CSF, a trend of decreasing glycosylation was observed from apoE2 > apoE3 > apoE4.					
32568201	9	45	theme	apoE2	1347:1351	arg1	apoE4					1363:1367	apoE2 > apoE3 > apoE4	1347:1367	apoE2 > apoE3 > apoE4	1347:1367	In plasma and CSF, a trend of decreasing glycosylation was observed from apoE2 > apoE3 > apoE4.					
32568201	4	46	theme	individuals	713:723	arg1	individuals					713:723	non-demented older individuals	694:723	non-demented older individuals (n = 22)	694:732	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	4	46	theme	individuals	713:723	arg1	group					685:689	a group	683:689	a group	683:689	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	4	46	theme	individuals	713:723	arg1	n = 22					726:731	n = 22	726:731	n = 22	726:731	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	4	47	dep	heterozygous	773:784	arg1	carriers					809:816	ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers	786:816	ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers	786:816	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	0	48	theme	Apolipoprotein	26:39	arg1	E					41:41	Apolipoprotein E Phenotyping and Glycotyping	26:69	E	41:41	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.					
32568201	6	49	theme	heterozygous	907:918	arg1	individuals					920:930	heterozygous individuals	907:930	heterozygous individuals	907:930	RESULTS In heterozygous individuals, the apoE3/E2, E4/E2, and E4/E3 isoform ratios were all significantly lower in plasma compared to CSF.					
32568201	3	50	theme	immunoassay	557:567	arg1	assay					576:580	a newly developed mass spectrometric immunoassay (MSIA) assay	520:580	a newly developed mass spectrometric immunoassay (MSIA) assay	520:580	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	9	51	theme	glycosylation	1315:1327	arg1	trend					1295:1299	a trend	1293:1299	a trend of decreasing glycosylation	1293:1327	In plasma and CSF, a trend of decreasing glycosylation was observed from apoE2 > apoE3 > apoE4.					
32568201	8	52	theme	total	1202:1206	arg1	apoE					1208:1211	total apoE	1202:1211	total apoE	1202:1211	The ratio of glycosylated to total apoE was greater in CSF compared to plasma for all apoE isoforms.					
32568201	0	53	dep	E	41:41	arg1	Phenotyping					43:53	Phenotyping	43:53	Phenotyping	43:53	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.					
32568201	8	54	theme	glycosylated	1186:1197	arg1	ratio					1177:1181	The ratio	1173:1181	The ratio of glycosylated to total apoE	1173:1211	The ratio of glycosylated to total apoE was greater in CSF compared to plasma for all apoE isoforms.					
32568201	8	54	theme	glycosylated	1186:1197	arg1	greater					1217:1223	greater	1217:1223	greater	1217:1223	The ratio of glycosylated to total apoE was greater in CSF compared to plasma for all apoE isoforms.					
32568201	3	55	theme	relative	412:419	arg1	abundance					421:429	the relative abundance	408:429	the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma	408:514	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	7	56	theme	O-linked	1065:1072	arg1	glycan					1074:1079	a single O-linked glycan	1056:1079	a single O-linked glycan	1056:1079	For all individuals, a single O-linked glycan was observed in plasma, while two glycans (of the same type) per apoE were observed in CSF.					
32568201	5	57	theme	glycan	823:828	arg1	structures					830:839	The glycan structures	819:839	The glycan structures	819:839	The glycan structures were further confirmed after treatment with sialidase.					
32568201	3	58	theme	mass	538:541	arg1	assay					576:580	a newly developed mass spectrometric immunoassay (MSIA) assay	520:580	a newly developed mass spectrometric immunoassay (MSIA) assay	520:580	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	3	59	theme	isoforms	447:454	arg1	abundance					421:429	the relative abundance	408:429	the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma	408:514	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	3	60	theme	spectrometric	543:555	arg1	assay					576:580	a newly developed mass spectrometric immunoassay (MSIA) assay	520:580	a newly developed mass spectrometric immunoassay (MSIA) assay	520:580	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	3	61	from	abundance	421:429	arg1	plasma					509:514	plasma	509:514	plasma	509:514	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	3	61	from	abundance	421:429	arg1	CSF					501:503	CSF	501:503	CSF	501:503	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	9	62	theme	apoE3	1355:1359	arg1	apoE4					1363:1367	apoE2 > apoE3 > apoE4	1347:1367	apoE2 > apoE3 > apoE4	1347:1367	In plasma and CSF, a trend of decreasing glycosylation was observed from apoE2 > apoE3 > apoE4.					
32568201	13	63	theme	AD	1777:1778	arg1	risk					1780:1783	AD risk	1777:1783	AD risk	1777:1783	Assessing apoE isoform-specific glycosylation by MSIA may help clarify brain apoE metabolism and AD risk.					
32568201	10	64	from	glycosylation	1416:1428	arg1	CSF					1433:1435	CSF	1433:1435	CSF	1433:1435	The difference in the percentage of secondary glycosylation in CSF was significantly greater in apoE4 compared to the other isoforms.					
32568201	0	65	dep	Assay	16:20	arg1	Glycosylation					101:113	Discovering Isoform-Specific Glycosylation	72:113	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.	0:148	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.					
32568201	3	66	theme	apoE	434:437	arg1	isoforms					447:454	apoE protein isoforms	434:454	apoE protein isoforms	434:454	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	4	67	from	group	685:689	arg1	CSF					674:676	CSF	674:676	CSF	674:676	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	4	67	from	group	685:689	arg1	plasma					663:668	plasma	663:668	plasma	663:668	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	6	68	from	RESULTS	896:902	arg1	individuals					920:930	heterozygous individuals	907:930	heterozygous individuals	907:930	RESULTS In heterozygous individuals, the apoE3/E2, E4/E2, and E4/E3 isoform ratios were all significantly lower in plasma compared to CSF.					
32568201	0	69	from	Glycosylation	101:113	arg1	Fluid					143:147	Cerebrospinal Fluid	129:147	Cerebrospinal Fluid	129:147	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.					
32568201	0	69	from	Glycosylation	101:113	arg1	Plasma					118:123	Plasma	118:123	Plasma	118:123	Simple and Fast Assay for Apolipoprotein E Phenotyping and Glycotyping: Discovering Isoform-Specific Glycosylation in Plasma and Cerebrospinal Fluid.					
32568201	2	70	theme	cerebrospinal	297:309	arg1	CSF					318:320	CSF	318:320	CSF	318:320	In cerebrospinal fluid (CSF), apoE is heavily glycosylated.					
32568201	2	70	theme	cerebrospinal	297:309	arg1	fluid					311:315	cerebrospinal fluid	297:315	cerebrospinal fluid (CSF)	297:321	In cerebrospinal fluid (CSF), apoE is heavily glycosylated.					
32568201	6	71	theme	isoform	964:970	arg1	RESULTS					896:902	RESULTS	896:902	RESULTS In heterozygous individuals	896:930	RESULTS In heterozygous individuals, the apoE3/E2, E4/E2, and E4/E3 isoform ratios were all significantly lower in plasma compared to CSF.					
32568201	6	71	theme	isoform	964:970	arg1	ratios					972:977	E4/E3 isoform ratios	958:977	E4/E3 isoform ratios	958:977	RESULTS In heterozygous individuals, the apoE3/E2, E4/E2, and E4/E3 isoform ratios were all significantly lower in plasma compared to CSF.					
32568201	7	72	theme	type	1136:1139	arg1	glycans					1115:1121	two glycans	1111:1121	two glycans (of the same type) per apoE	1111:1149	For all individuals, a single O-linked glycan was observed in plasma, while two glycans (of the same type) per apoE were observed in CSF.					
32568201	8	73	theme	apoE	1259:1262	arg1	isoforms					1264:1271	all apoE isoforms	1255:1271	all apoE isoforms	1255:1271	The ratio of glycosylated to total apoE was greater in CSF compared to plasma for all apoE isoforms.					
32568201	3	74	theme	MSIA	570:573	arg1	assay					576:580	a newly developed mass spectrometric immunoassay (MSIA) assay	520:580	a newly developed mass spectrometric immunoassay (MSIA) assay	520:580	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	3	75	theme	glycosylation	475:487	arg1	patterns					489:496	their specific glycosylation patterns	460:496	their specific glycosylation patterns	460:496	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	4	76	theme	isoform-specific	615:630	arg1	glycosylation					632:644	isoform-specific glycosylation	615:644	isoform-specific glycosylation	615:644	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	4	76	theme	isoform-specific	615:630	arg1	METHODS					583:589	METHODS	583:589	METHODS Total glycosylation and isoform-specific glycosylation	583:644	METHODS Total glycosylation and isoform-specific glycosylation were analyzed in plasma and CSF from a group of non-demented older individuals (n = 22), consisting of homozygous ɛ3 and ɛ4 or heterozygous ɛ3/ɛ4, ɛ2/ɛ3, or ɛ2/ɛ4 carriers.					
32568201	6	77	theme	E4/E3	958:962	arg1	RESULTS					896:902	RESULTS	896:902	RESULTS In heterozygous individuals	896:930	RESULTS In heterozygous individuals, the apoE3/E2, E4/E2, and E4/E3 isoform ratios were all significantly lower in plasma compared to CSF.					
32568201	6	77	theme	E4/E3	958:962	arg1	ratios					972:977	E4/E3 isoform ratios	958:977	E4/E3 isoform ratios	958:977	RESULTS In heterozygous individuals, the apoE3/E2, E4/E2, and E4/E3 isoform ratios were all significantly lower in plasma compared to CSF.					
32568201	7	78	located	observed	1156:1163	arg1	CSF					1168:1170	CSF	1168:1170	CSF	1168:1170	For all individuals, a single O-linked glycan was observed in plasma, while two glycans (of the same type) per apoE were observed in CSF.					
32568201	7	78	located	observed	1156:1163	arg2	glycans					1115:1121	two glycans	1111:1121	two glycans (of the same type) per apoE	1111:1149	For all individuals, a single O-linked glycan was observed in plasma, while two glycans (of the same type) per apoE were observed in CSF.					
32568201	3	79	theme	patterns	489:496	arg1	abundance					421:429	the relative abundance	408:429	the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma	408:514	OBJECTIVE To determine the impact of APOE genotype on the relative abundance of apoE protein isoforms and their specific glycosylation patterns in CSF and plasma via a newly developed mass spectrometric immunoassay (MSIA) assay.					
32568201	13	80	theme	brain	1751:1755	arg1	metabolism					1762:1771	brain apoE metabolism	1751:1771	brain apoE metabolism	1751:1771	Assessing apoE isoform-specific glycosylation by MSIA may help clarify brain apoE metabolism and AD risk.					
34831278	0	0	theme	Cell	62:65	arg1	Lines					67:71	Acute Myeloid Leukemia (AML) Cell Lines	33:71	Acute Myeloid Leukemia (AML) Cell Lines	33:71	Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines.					
34831278	9	1	theme	structures	1316:1325	arg1	expression					1295:1304	the varying expression	1283:1304	the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors	1283:1482	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	8	2	theme	hematopoietic	1137:1149	arg1	factors					1165:1171	hematopoietic transcription factors	1137:1171	hematopoietic transcription factors	1137:1171	By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells.					
34831278	3	3	contain	have	366:369	arg2	implications					380:391	proposed implications	371:391	proposed implications of protein glycosylation	371:416	Recently, several studies have proposed implications of protein glycosylation in the pathobiology of AML including chemoresistance.					
34831278	3	3	contain	have	366:369	arg1	studies					358:364	several studies	350:364	several studies	350:364	Recently, several studies have proposed implications of protein glycosylation in the pathobiology of AML including chemoresistance.					
34831278	8	4	theme	candidate	1200:1208	arg1	which					1190:1194	which	1190:1194	which	1190:1194	By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells.					
34831278	8	4	theme	candidate	1200:1208	arg1	drivers					1210:1216	candidate drivers	1200:1216	candidate drivers of the glycan phenotype of these cells	1200:1255	By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells.					
34831278	7	5	theme	distinct	925:932	arg1	fingerprints					941:952	distinct glycan fingerprints	925:952	distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype	925:1030	AML cell lines exhibited distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype as classified by the FAB system.					
34831278	5	6	theme	exploratory	633:643	arg1	study					655:659	an exploratory glycomics study	630:659	an exploratory glycomics study characterizing 21 widely used AML cell lines	630:704	To advance this poorly studied field, we performed an exploratory glycomics study characterizing 21 widely used AML cell lines.					
34831278	7	7	theme	glycan	976:981	arg1	traits					983:988	relevant glycan traits	967:988	relevant glycan traits correlating with their cellular phenotype	967:1030	AML cell lines exhibited distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype as classified by the FAB system.					
34831278	9	8	theme	poor	1399:1402	arg1	prognosis					1404:1412	poor prognosis	1399:1412	poor prognosis	1399:1412	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	9	9	theme	transcription	1462:1474	arg1	factors					1476:1482	transcription factors	1462:1482	transcription factors	1462:1482	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	0	10	theme	Lines	67:71	arg1	O-Glycomics					18:28	O-Glycomics	18:28	O-Glycomics	18:28	Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines.					
34831278	0	10	theme	Lines	67:71	arg1	N-					11:12	N-	11:12	N-	11:12	Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines.					
34831278	9	11	theme	repertoire	1544:1553	arg1	regulation					1515:1524	the regulation	1511:1524	the regulation of the AML glycan repertoire	1511:1553	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	5	12	theme	glycomics	645:653	arg1	study					655:659	an exploratory glycomics study	630:659	an exploratory glycomics study characterizing 21 widely used AML cell lines	630:704	To advance this poorly studied field, we performed an exploratory glycomics study characterizing 21 widely used AML cell lines.					
34831278	2	13	theme	high	257:260	arg1	rate					262:265	a high rate	255:265	a high rate of relapsed or refractory disease	255:299	Although patients are usually treated effectively by chemotherapy, a high rate of relapsed or refractory disease poses a major hurdle in its treatment.					
34831278	4	14	theme	disease	570:576	arg1	epitopes					538:545	specific glycan epitopes	522:545	specific glycan epitopes	522:545	Accordingly, associations have been found between specific glycan epitopes and the outcome of the disease.					
34831278	4	14	theme	disease	570:576	arg1	outcome					555:561	the outcome	551:561	the outcome of the disease	551:576	Accordingly, associations have been found between specific glycan epitopes and the outcome of the disease.					
34831278	6	15	theme	O-linked	882:889	arg1	glycans					891:897	O-linked glycans	882:897	O-linked glycans	882:897	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	5	16	theme	studied	602:608	arg1	field					610:614	this poorly studied field	590:614	this poorly studied field	590:614	To advance this poorly studied field, we performed an exploratory glycomics study characterizing 21 widely used AML cell lines.					
34831278	3	17	theme	AML	441:443	arg1	pathobiology					425:436	the pathobiology	421:436	the pathobiology of AML including chemoresistance	421:469	Recently, several studies have proposed implications of protein glycosylation in the pathobiology of AML including chemoresistance.					
34831278	9	18	theme	cell	1355:1358	arg1	lines					1360:1364	AML cell lines	1351:1364	AML cell lines	1351:1364	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	9	18	theme	cell	1355:1358	arg1	those					1377:1381	those	1377:1381	those	1377:1381	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	1	19	theme	dysregulated	125:136	arg1	expansion					138:146	a dysregulated expansion	123:146	a dysregulated expansion of poorly differentiated myeloid cells	123:185	Acute myeloid leukemia (AML) is characterized by a dysregulated expansion of poorly differentiated myeloid cells.					
34831278	3	20	theme	proposed	371:378	arg1	implications					380:391	proposed implications	371:391	proposed implications of protein glycosylation	371:416	Recently, several studies have proposed implications of protein glycosylation in the pathobiology of AML including chemoresistance.					
34831278	6	21	theme	tandem	786:791	arg1	spectrometry					798:809	tandem mass spectrometry	786:809	tandem mass spectrometry (PGC nano-LC-MS2)	786:827	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	6	21	theme	tandem	786:791	arg1	nano-LC-MS2					816:826	PGC nano-LC-MS2	812:826	PGC nano-LC-MS2	812:826	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	8	22	theme	glycan	1225:1230	arg1	phenotype					1232:1240	the glycan phenotype	1221:1240	the glycan phenotype of these cells	1221:1255	By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells.					
34831278	9	23	theme	AML	1351:1353	arg1	lines					1360:1364	AML cell lines	1351:1364	AML cell lines	1351:1364	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	9	23	theme	AML	1351:1353	arg1	those					1377:1381	those	1377:1381	those	1377:1381	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	8	24	theme	transcriptomics	1081:1095	arg1	data					1097:1100	transcriptomics data	1081:1100	transcriptomics data	1081:1100	By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells.					
34831278	2	25	from	hurdle	315:320	arg1	treatment					329:337	its treatment	325:337	its treatment	325:337	Although patients are usually treated effectively by chemotherapy, a high rate of relapsed or refractory disease poses a major hurdle in its treatment.					
34831278	2	26	theme	refractory	282:291	arg1	disease					293:299	relapsed or refractory disease	270:299	relapsed or refractory disease	270:299	Although patients are usually treated effectively by chemotherapy, a high rate of relapsed or refractory disease poses a major hurdle in its treatment.					
34831278	3	27	theme	several	350:356	arg1	studies					358:364	several studies	350:364	several studies	350:364	Recently, several studies have proposed implications of protein glycosylation in the pathobiology of AML including chemoresistance.					
34831278	7	28	theme	cellular	1013:1020	arg1	phenotype					1022:1030	their cellular phenotype	1007:1030	their cellular phenotype	1007:1030	AML cell lines exhibited distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype as classified by the FAB system.					
34831278	3	29	theme	protein	396:402	arg1	glycosylation					404:416	protein glycosylation	396:416	protein glycosylation	396:416	Recently, several studies have proposed implications of protein glycosylation in the pathobiology of AML including chemoresistance.					
34831278	9	30	theme	lines	1360:1364	arg1	number					1341:1346	a high number	1334:1346	a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors	1334:1482	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	9	30	theme	lines	1360:1364	arg1	those					1377:1381	those	1377:1381	those	1377:1381	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	5	31	theme	used	686:689	arg1	lines					700:704	21 widely used AML cell lines	676:704	21 widely used AML cell lines	676:704	To advance this poorly studied field, we performed an exploratory glycomics study characterizing 21 widely used AML cell lines.					
34831278	6	32	theme	mass	793:796	arg1	spectrometry					798:809	tandem mass spectrometry	786:809	tandem mass spectrometry (PGC nano-LC-MS2)	786:827	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	6	32	theme	mass	793:796	arg1	nano-LC-MS2					816:826	PGC nano-LC-MS2	812:826	PGC nano-LC-MS2	812:826	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	2	33	theme	relapsed	270:277	arg1	disease					293:299	relapsed or refractory disease	270:299	relapsed or refractory disease	270:299	Although patients are usually treated effectively by chemotherapy, a high rate of relapsed or refractory disease poses a major hurdle in its treatment.					
34831278	6	34	theme	PGC	812:814	arg1	spectrometry					798:809	tandem mass spectrometry	786:809	tandem mass spectrometry (PGC nano-LC-MS2)	786:827	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	6	34	theme	PGC	812:814	arg1	nano-LC-MS2					816:826	PGC nano-LC-MS2	812:826	PGC nano-LC-MS2	812:826	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	1	35	theme	differentiated	158:171	arg1	cells					181:185	poorly differentiated myeloid cells	151:185	poorly differentiated myeloid cells	151:185	Acute myeloid leukemia (AML) is characterized by a dysregulated expansion of poorly differentiated myeloid cells.					
34831278	8	36	theme	phenotype	1232:1240	arg1	which					1190:1194	which	1190:1194	which	1190:1194	By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells.					
34831278	8	36	theme	phenotype	1232:1240	arg1	drivers					1210:1216	candidate drivers	1200:1216	candidate drivers of the glycan phenotype of these cells	1200:1255	By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells.					
34831278	5	37	theme	AML	691:693	arg1	lines					700:704	21 widely used AML cell lines	676:704	21 widely used AML cell lines	676:704	To advance this poorly studied field, we performed an exploratory glycomics study characterizing 21 widely used AML cell lines.					
34831278	1	38	theme	myeloid	80:86	arg1	AML					98:100	AML	98:100	AML	98:100	Acute myeloid leukemia (AML) is characterized by a dysregulated expansion of poorly differentiated myeloid cells.					
34831278	1	38	theme	myeloid	80:86	arg1	leukemia					88:95	Acute myeloid leukemia	74:95	Acute myeloid leukemia (AML)	74:101	Acute myeloid leukemia (AML) is characterized by a dysregulated expansion of poorly differentiated myeloid cells.					
34831278	1	39	theme	myeloid	173:179	arg1	cells					181:185	poorly differentiated myeloid cells	151:185	poorly differentiated myeloid cells	151:185	Acute myeloid leukemia (AML) is characterized by a dysregulated expansion of poorly differentiated myeloid cells.					
34831278	0	40	theme	Acute	33:37	arg1	AML					57:59	AML	57:59	AML	57:59	Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines.					
34831278	0	40	theme	Acute	33:37	arg1	Leukemia					47:54	Acute Myeloid Leukemia	33:54	Acute Myeloid Leukemia (AML) Cell Lines	33:71	Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines.					
34831278	9	41	theme	AML	1533:1535	arg1	repertoire					1544:1553	the AML glycan repertoire	1529:1553	the AML glycan repertoire	1529:1553	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	5	42	theme	cell	695:698	arg1	lines					700:704	21 widely used AML cell lines	676:704	21 widely used AML cell lines	676:704	To advance this poorly studied field, we performed an exploratory glycomics study characterizing 21 widely used AML cell lines.					
34831278	1	43	theme	cells	181:185	arg1	expansion					138:146	a dysregulated expansion	123:146	a dysregulated expansion of poorly differentiated myeloid cells	123:185	Acute myeloid leukemia (AML) is characterized by a dysregulated expansion of poorly differentiated myeloid cells.					
34831278	3	44	theme	glycosylation	404:416	arg1	implications					380:391	proposed implications	371:391	proposed implications of protein glycosylation	371:416	Recently, several studies have proposed implications of protein glycosylation in the pathobiology of AML including chemoresistance.					
34831278	8	45	theme	specific	1103:1110	arg1	glycosyltransferases					1112:1131	specific glycosyltransferases	1103:1131	specific glycosyltransferases	1103:1131	By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells.					
34831278	6	46	theme	carbon	753:758	arg1	chromatography					760:773	porous graphitized carbon chromatography	734:773	porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2)	734:827	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	6	47	link	O-linked	882:889	arg1	glycans					891:897	O-linked glycans	882:897	O-linked glycans	882:897	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	0	48	theme	Leukemia	47:54	arg1	Lines					67:71	Acute Myeloid Leukemia (AML) Cell Lines	33:71	Acute Myeloid Leukemia (AML) Cell Lines	33:71	Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines.					
34831278	7	49	theme	FAB	1053:1055	arg1	system					1057:1062	the FAB system	1049:1062	the FAB system	1049:1062	AML cell lines exhibited distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype as classified by the FAB system.					
34831278	6	50	theme	chromatography	760:773	arg1	benefits					722:729	the benefits	718:729	the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2)	718:827	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	2	51	theme	major	309:313	arg1	hurdle					315:320	a major hurdle	307:320	a major hurdle in its treatment	307:337	Although patients are usually treated effectively by chemotherapy, a high rate of relapsed or refractory disease poses a major hurdle in its treatment.					
34831278	0	52	theme	Myeloid	39:45	arg1	AML					57:59	AML	57:59	AML	57:59	Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines.					
34831278	0	52	theme	Myeloid	39:45	arg1	Leukemia					47:54	Acute Myeloid Leukemia	33:54	Acute Myeloid Leukemia (AML) Cell Lines	33:71	Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines.					
34831278	4	53	theme	glycan	531:536	arg1	epitopes					538:545	specific glycan epitopes	522:545	specific glycan epitopes	522:545	Accordingly, associations have been found between specific glycan epitopes and the outcome of the disease.					
34831278	9	54	theme	varying	1287:1293	arg1	expression					1295:1304	the varying expression	1283:1304	the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors	1283:1482	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	6	55	theme	porous	734:739	arg1	chromatography					760:773	porous graphitized carbon chromatography	734:773	porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2)	734:827	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	8	56	theme	cells	1251:1255	arg1	phenotype					1232:1240	the glycan phenotype	1221:1240	the glycan phenotype of these cells	1221:1255	By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells.					
34831278	7	57	theme	cell	904:907	arg1	lines					909:913	AML cell lines	900:913	AML cell lines	900:913	AML cell lines exhibited distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype as classified by the FAB system.					
34831278	4	58	theme	specific	522:529	arg1	epitopes					538:545	specific glycan epitopes	522:545	specific glycan epitopes	522:545	Accordingly, associations have been found between specific glycan epitopes and the outcome of the disease.					
34831278	7	59	theme	glycan	934:939	arg1	fingerprints					941:952	distinct glycan fingerprints	925:952	distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype	925:1030	AML cell lines exhibited distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype as classified by the FAB system.					
34831278	9	60	theme	glycan	1537:1542	arg1	repertoire					1544:1553	the AML glycan repertoire	1529:1553	the AML glycan repertoire	1529:1553	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	1	61	theme	Acute	74:78	arg1	AML					98:100	AML	98:100	AML	98:100	Acute myeloid leukemia (AML) is characterized by a dysregulated expansion of poorly differentiated myeloid cells.					
34831278	1	61	theme	Acute	74:78	arg1	leukemia					88:95	Acute myeloid leukemia	74:95	Acute myeloid leukemia (AML)	74:101	Acute myeloid leukemia (AML) is characterized by a dysregulated expansion of poorly differentiated myeloid cells.					
34831278	6	62	theme	graphitized	741:751	arg1	chromatography					760:773	porous graphitized carbon chromatography	734:773	porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2)	734:827	Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans.					
34831278	8	63	theme	transcription	1151:1163	arg1	factors					1165:1171	hematopoietic transcription factors	1137:1171	hematopoietic transcription factors	1137:1171	By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells.					
34831278	2	64	theme	disease	293:299	arg1	rate					262:265	a high rate	255:265	a high rate of relapsed or refractory disease	255:299	Although patients are usually treated effectively by chemotherapy, a high rate of relapsed or refractory disease poses a major hurdle in its treatment.					
34831278	7	65	theme	relevant	967:974	arg1	traits					983:988	relevant glycan traits	967:988	relevant glycan traits correlating with their cellular phenotype	967:1030	AML cell lines exhibited distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype as classified by the FAB system.					
34831278	7	66	theme	AML	900:902	arg1	lines					909:913	AML cell lines	900:913	AML cell lines	900:913	AML cell lines exhibited distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype as classified by the FAB system.					
34831278	9	67	theme	glycan	1309:1314	arg1	structures					1316:1325	glycan structures	1309:1325	glycan structures	1309:1325	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	9	68	theme	high	1336:1339	arg1	number					1341:1346	a high number	1334:1346	a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors	1334:1482	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
34831278	9	68	theme	high	1336:1339	arg1	those					1377:1381	those	1377:1381	those	1377:1381	In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.					
32070621	3	0	theme	insufficient	561:572	arg1	power					584:588	the insufficient resolving power	557:588	the insufficient resolving power of most IMS-MS instruments	557:615	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	8	1	with	ions	1335:1338	arg1	glycan					1347:1352	a glycan	1345:1352	a glycan	1345:1352	The best IMS separation of isomeric glycans was achieved in some cases by incorporating multiple ions with a glycan, such as the complex [glycan + Ca + Cl]+.					
32070621	3	2	theme	instruments	605:615	arg1	power					584:588	the insufficient resolving power	557:588	the insufficient resolving power of most IMS-MS instruments	557:615	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	4	3	theme	IMS	732:734	arg1	power					746:750	the low IMS resolving power	724:750	the low IMS resolving power	724:750	To mitigate the low IMS resolving power, metal ion incorporation has been employed to enhance the separation of isomeric glycans.					
32070621	7	4	theme	IMS	1170:1172	arg1	separation					1174:1183	Baseline IMS separation	1161:1183	Baseline IMS separation	1161:1183	Baseline IMS separation was achieved for 76% of these isomeric glycan pairs.					
32070621	3	5	theme	most	593:596	arg1	instruments					605:615	most IMS-MS instruments	593:615	most IMS-MS instruments	593:615	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	9	6	theme	specific	1478:1485	arg1	classes					1496:1502	specific compound classes	1478:1502	specific compound classes	1478:1502	In addition, the well-known IMS-MS measurement trendlines, often used to identify specific compound classes, were preserved for glycans even for all the 270 glycan-metal ion complexes observed in IMS-MS spectra.					
32070621	8	7	theme	best	1242:1245	arg1	separation					1251:1260	The best IMS separation	1238:1260	The best IMS separation of isomeric glycans	1238:1280	The best IMS separation of isomeric glycans was achieved in some cases by incorporating multiple ions with a glycan, such as the complex [glycan + Ca + Cl]+.					
32070621	7	8	theme	pairs	1231:1235	arg1	pairs					1231:1235	these isomeric glycan pairs	1209:1235	these isomeric glycan pairs	1209:1235	Baseline IMS separation was achieved for 76% of these isomeric glycan pairs.					
32070621	7	8	theme	pairs	1231:1235	arg1	%					1204:1204	76%	1202:1204	76% of these isomeric glycan pairs	1202:1235	Baseline IMS separation was achieved for 76% of these isomeric glycan pairs.					
32070621	1	9	theme	complex	196:202	arg1	systems					215:221	complex biological systems	196:221	complex biological systems	196:221	Glycosylated proteins are an essential class of molecules playing critical roles in complex biological systems.					
32070621	5	10	theme	glycan-metal	904:915	arg1	complexes					921:929	many different glycan-metal ion complexes	889:929	many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans	889:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	8	11	theme	isomeric	1265:1272	arg1	glycans					1274:1280	isomeric glycans	1265:1280	isomeric glycans	1265:1280	The best IMS separation of isomeric glycans was achieved in some cases by incorporating multiple ions with a glycan, such as the complex [glycan + Ca + Cl]+.					
32070621	6	12	theme	glycan-metal	1028:1039	arg1	complexes					1045:1053	optimum glycan-metal ion complexes	1020:1053	optimum glycan-metal ion complexes	1020:1053	By selecting optimum glycan-metal ion complexes, partial IMS separation was realized for all the 21 isomeric glycan pairs used in the experimental study.					
32070621	3	13	theme	comprehensive	659:671	arg1	characterization					683:698	the comprehensive structure characterization	655:698	the comprehensive structure characterization of glycans	655:709	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	5	14	theme	complexes	921:929	arg1	investigation					872:884	a systematic investigation	859:884	a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans	859:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	5	15	theme	different	979:987	arg1	glycans					998:1004	different isomeric glycans	979:1004	different isomeric glycans	979:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	3	16	theme	glycans	703:709	arg1	characterization					683:698	the comprehensive structure characterization	655:698	the comprehensive structure characterization of glycans	655:709	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	5	17	from	attempt	937:943	arg1	investigation					872:884	a systematic investigation	859:884	a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans	859:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	5	18	theme	glycans	998:1004	arg1	separation					965:974	the IMS separation	957:974	the IMS separation of different isomeric glycans	957:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	9	19	located	observed	1580:1587	arg2	complexes					1570:1578	all the 270 glycan-metal ion complexes	1541:1578	all the 270 glycan-metal ion complexes observed in IMS-MS spectra	1541:1605	In addition, the well-known IMS-MS measurement trendlines, often used to identify specific compound classes, were preserved for glycans even for all the 270 glycan-metal ion complexes observed in IMS-MS spectra.					
32070621	9	19	located	observed	1580:1587	arg1	spectra					1599:1605	IMS-MS spectra	1592:1605	IMS-MS spectra	1592:1605	In addition, the well-known IMS-MS measurement trendlines, often used to identify specific compound classes, were preserved for glycans even for all the 270 glycan-metal ion complexes observed in IMS-MS spectra.					
32070621	4	20	theme	metal	753:757	arg1	incorporation					763:775	metal ion incorporation	753:775	metal ion incorporation	753:775	To mitigate the low IMS resolving power, metal ion incorporation has been employed to enhance the separation of isomeric glycans.					
32070621	5	21	theme	many	889:892	arg1	complexes					921:929	many different glycan-metal ion complexes	889:929	many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans	889:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	2	22	dep	compositions	320:331	arg1	the					300:302	the	300:302	the	300:302	Understanding their biological functions remains extremely difficult due to the extremely broad compositions and structure variations of glycans.					
32070621	0	23	theme	tube	75:78	arg1	spectrometry					98:109	drift tube ion mobility-mass spectrometry	69:109	drift tube ion mobility-mass spectrometry	69:109	Improving glycan isomeric separation via metal ion incorporation for drift tube ion mobility-mass spectrometry.					
32070621	9	24	theme	ion	1566:1568	arg1	complexes					1570:1578	all the 270 glycan-metal ion complexes	1541:1578	all the 270 glycan-metal ion complexes observed in IMS-MS spectra	1541:1605	In addition, the well-known IMS-MS measurement trendlines, often used to identify specific compound classes, were preserved for glycans even for all the 270 glycan-metal ion complexes observed in IMS-MS spectra.					
32070621	3	25	theme	ion	398:400	arg1	spectrometry					411:422	ion mobility spectrometry	398:422	ion mobility spectrometry	398:422	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	4	26	theme	glycans	833:839	arg1	separation					810:819	the separation	806:819	the separation of isomeric glycans	806:839	To mitigate the low IMS resolving power, metal ion incorporation has been employed to enhance the separation of isomeric glycans.					
32070621	0	27	theme	mobility-mass	84:96	arg1	spectrometry					98:109	drift tube ion mobility-mass spectrometry	69:109	drift tube ion mobility-mass spectrometry	69:109	Improving glycan isomeric separation via metal ion incorporation for drift tube ion mobility-mass spectrometry.					
32070621	2	28	theme	broad	314:318	arg1	compositions					320:331	extremely broad compositions	304:331	extremely broad compositions	304:331	Understanding their biological functions remains extremely difficult due to the extremely broad compositions and structure variations of glycans.					
32070621	6	29	theme	partial	1056:1062	arg1	separation					1068:1077	partial IMS separation	1056:1077	partial IMS separation	1056:1077	By selecting optimum glycan-metal ion complexes, partial IMS separation was realized for all the 21 isomeric glycan pairs used in the experimental study.					
32070621	3	30	theme	promising	468:476	arg1	technique					478:486	a promising technique	466:486	a promising technique in glycan structure characterization and composition identification	466:554	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	9	31	theme	IMS-MS	1592:1597	arg1	spectra					1599:1605	IMS-MS spectra	1592:1605	IMS-MS spectra	1592:1605	In addition, the well-known IMS-MS measurement trendlines, often used to identify specific compound classes, were preserved for glycans even for all the 270 glycan-metal ion complexes observed in IMS-MS spectra.					
32070621	9	32	theme	glycan-metal	1553:1564	arg1	complexes					1570:1578	all the 270 glycan-metal ion complexes	1541:1578	all the 270 glycan-metal ion complexes observed in IMS-MS spectra	1541:1605	In addition, the well-known IMS-MS measurement trendlines, often used to identify specific compound classes, were preserved for glycans even for all the 270 glycan-metal ion complexes observed in IMS-MS spectra.					
32070621	1	33	theme	essential	141:149	arg1	proteins					125:132	Glycosylated proteins	112:132	Glycosylated proteins	112:132	Glycosylated proteins are an essential class of molecules playing critical roles in complex biological systems.					
32070621	1	33	theme	essential	141:149	arg1	class					151:155	an essential class	138:155	an essential class of molecules playing critical roles in complex biological systems	138:221	Glycosylated proteins are an essential class of molecules playing critical roles in complex biological systems.					
32070621	3	34	theme	spectrometry	411:422	arg1	combination					383:393	the combination	379:393	the combination of ion mobility spectrometry and mass spectrometry (IMS-MS)	379:453	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	3	35	theme	structure	498:506	arg1	characterization					508:523	glycan structure characterization	491:523	glycan structure characterization	491:523	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	0	36	theme	isomeric	17:24	arg1	separation					26:35	glycan isomeric separation	10:35	glycan isomeric separation	10:35	Improving glycan isomeric separation via metal ion incorporation for drift tube ion mobility-mass spectrometry.					
32070621	1	37	theme	Glycosylated	112:123	arg1	proteins					125:132	Glycosylated proteins	112:132	Glycosylated proteins	112:132	Glycosylated proteins are an essential class of molecules playing critical roles in complex biological systems.					
32070621	1	37	theme	Glycosylated	112:123	arg1	class					151:155	an essential class	138:155	an essential class of molecules playing critical roles in complex biological systems	138:221	Glycosylated proteins are an essential class of molecules playing critical roles in complex biological systems.					
32070621	6	38	theme	glycan	1116:1121	arg1	pairs					1123:1127	all the 21 isomeric glycan pairs	1096:1127	all the 21 isomeric glycan pairs used in the experimental study	1096:1158	By selecting optimum glycan-metal ion complexes, partial IMS separation was realized for all the 21 isomeric glycan pairs used in the experimental study.					
32070621	0	39	theme	ion	47:49	arg1	incorporation					51:63	metal ion incorporation	41:63	metal ion incorporation for drift tube ion mobility-mass spectrometry	41:109	Improving glycan isomeric separation via metal ion incorporation for drift tube ion mobility-mass spectrometry.					
32070621	9	40	theme	well-known	1413:1422	arg1	trendlines					1443:1452	the well-known IMS-MS measurement trendlines	1409:1452	the well-known IMS-MS measurement trendlines	1409:1452	In addition, the well-known IMS-MS measurement trendlines, often used to identify specific compound classes, were preserved for glycans even for all the 270 glycan-metal ion complexes observed in IMS-MS spectra.					
32070621	3	41	theme	resolving	574:582	arg1	power					584:588	the insufficient resolving power	557:588	the insufficient resolving power of most IMS-MS instruments	557:615	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	9	42	theme	measurement	1431:1441	arg1	trendlines					1443:1452	the well-known IMS-MS measurement trendlines	1409:1452	the well-known IMS-MS measurement trendlines	1409:1452	In addition, the well-known IMS-MS measurement trendlines, often used to identify specific compound classes, were preserved for glycans even for all the 270 glycan-metal ion complexes observed in IMS-MS spectra.					
32070621	3	43	dep	characterization	508:523	arg1	identification					541:554	identification	541:554	identification	541:554	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	3	44	from	technique	478:486	arg1	composition					529:539	composition	529:539	composition	529:539	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	3	44	from	technique	478:486	arg1	characterization					508:523	glycan structure characterization	491:523	glycan structure characterization	491:523	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	3	45	theme	IMS-MS	598:603	arg1	instruments					605:615	most IMS-MS instruments	593:615	most IMS-MS instruments	593:615	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	1	46	theme	molecules	160:168	arg1	proteins					125:132	Glycosylated proteins	112:132	Glycosylated proteins	112:132	Glycosylated proteins are an essential class of molecules playing critical roles in complex biological systems.					
32070621	1	46	theme	molecules	160:168	arg1	class					151:155	an essential class	138:155	an essential class of molecules playing critical roles in complex biological systems	138:221	Glycosylated proteins are an essential class of molecules playing critical roles in complex biological systems.					
32070621	7	47	theme	Baseline	1161:1168	arg1	separation					1174:1183	Baseline IMS separation	1161:1183	Baseline IMS separation	1161:1183	Baseline IMS separation was achieved for 76% of these isomeric glycan pairs.					
32070621	1	48	theme	critical	178:185	arg1	roles					187:191	critical roles	178:191	critical roles	178:191	Glycosylated proteins are an essential class of molecules playing critical roles in complex biological systems.					
32070621	4	49	theme	low	728:730	arg1	power					746:750	the low IMS resolving power	724:750	the low IMS resolving power	724:750	To mitigate the low IMS resolving power, metal ion incorporation has been employed to enhance the separation of isomeric glycans.					
32070621	9	50	theme	compound	1487:1494	arg1	classes					1496:1502	specific compound classes	1478:1502	specific compound classes	1478:1502	In addition, the well-known IMS-MS measurement trendlines, often used to identify specific compound classes, were preserved for glycans even for all the 270 glycan-metal ion complexes observed in IMS-MS spectra.					
32070621	4	51	theme	resolving	736:744	arg1	power					746:750	the low IMS resolving power	724:750	the low IMS resolving power	724:750	To mitigate the low IMS resolving power, metal ion incorporation has been employed to enhance the separation of isomeric glycans.					
32070621	2	52	theme	structure	337:345	arg1	variations					347:356	structure variations	337:356	structure variations	337:356	Understanding their biological functions remains extremely difficult due to the extremely broad compositions and structure variations of glycans.					
32070621	5	53	theme	different	894:902	arg1	complexes					921:929	many different glycan-metal ion complexes	889:929	many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans	889:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	8	54	theme	IMS	1247:1249	arg1	separation					1251:1260	The best IMS separation	1238:1260	The best IMS separation of isomeric glycans	1238:1280	The best IMS separation of isomeric glycans was achieved in some cases by incorporating multiple ions with a glycan, such as the complex [glycan + Ca + Cl]+.					
32070621	6	55	theme	ion	1041:1043	arg1	complexes					1045:1053	optimum glycan-metal ion complexes	1020:1053	optimum glycan-metal ion complexes	1020:1053	By selecting optimum glycan-metal ion complexes, partial IMS separation was realized for all the 21 isomeric glycan pairs used in the experimental study.					
32070621	5	56	theme	ion	917:919	arg1	complexes					921:929	many different glycan-metal ion complexes	889:929	many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans	889:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	6	57	theme	optimum	1020:1026	arg1	complexes					1045:1053	optimum glycan-metal ion complexes	1020:1053	optimum glycan-metal ion complexes	1020:1053	By selecting optimum glycan-metal ion complexes, partial IMS separation was realized for all the 21 isomeric glycan pairs used in the experimental study.					
32070621	8	58	theme	multiple	1326:1333	arg1	[glycan + Ca + Cl					1375:1391	the complex [glycan + Ca + Cl	1363:1391	the complex [glycan + Ca + Cl	1363:1391	The best IMS separation of isomeric glycans was achieved in some cases by incorporating multiple ions with a glycan, such as the complex [glycan + Ca + Cl]+.					
32070621	8	58	theme	multiple	1326:1333	arg1	ions					1335:1338	multiple ions	1326:1338	multiple ions	1326:1338	The best IMS separation of isomeric glycans was achieved in some cases by incorporating multiple ions with a glycan, such as the complex [glycan + Ca + Cl]+.					
32070621	2	59	theme	biological	244:253	arg1	functions					255:263	their biological functions	238:263	their biological functions	238:263	Understanding their biological functions remains extremely difficult due to the extremely broad compositions and structure variations of glycans.					
32070621	5	60	from	complexes	921:929	arg1	attempt					937:943	an attempt to optimize the IMS separation of different isomeric glycans	934:1004	an attempt to optimize the IMS separation of different isomeric glycans	934:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	8	61	theme	glycans	1274:1280	arg1	separation					1251:1260	The best IMS separation	1238:1260	The best IMS separation of isomeric glycans	1238:1280	The best IMS separation of isomeric glycans was achieved in some cases by incorporating multiple ions with a glycan, such as the complex [glycan + Ca + Cl]+.					
32070621	7	62	theme	glycan	1224:1229	arg1	pairs					1231:1235	these isomeric glycan pairs	1209:1235	these isomeric glycan pairs	1209:1235	Baseline IMS separation was achieved for 76% of these isomeric glycan pairs.					
32070621	5	63	theme	isomeric	989:996	arg1	glycans					998:1004	different isomeric glycans	979:1004	different isomeric glycans	979:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	0	64	theme	drift	69:73	arg1	spectrometry					98:109	drift tube ion mobility-mass spectrometry	69:109	drift tube ion mobility-mass spectrometry	69:109	Improving glycan isomeric separation via metal ion incorporation for drift tube ion mobility-mass spectrometry.					
32070621	3	65	theme	structure	673:681	arg1	characterization					683:698	the comprehensive structure characterization	655:698	the comprehensive structure characterization of glycans	655:709	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	1	66	theme	biological	204:213	arg1	systems					215:221	complex biological systems	196:221	complex biological systems	196:221	Glycosylated proteins are an essential class of molecules playing critical roles in complex biological systems.					
32070621	3	67	theme	mass	428:431	arg1	IMS-MS					447:452	IMS-MS	447:452	IMS-MS	447:452	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	3	67	theme	mass	428:431	arg1	spectrometry					433:444	mass spectrometry	428:444	mass spectrometry (IMS-MS)	428:453	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	0	68	theme	ion	80:82	arg1	spectrometry					98:109	drift tube ion mobility-mass spectrometry	69:109	drift tube ion mobility-mass spectrometry	69:109	Improving glycan isomeric separation via metal ion incorporation for drift tube ion mobility-mass spectrometry.					
32070621	5	69	theme	IMS	961:963	arg1	separation					965:974	the IMS separation	957:974	the IMS separation of different isomeric glycans	957:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	4	70	theme	ion	759:761	arg1	incorporation					763:775	metal ion incorporation	753:775	metal ion incorporation	753:775	To mitigate the low IMS resolving power, metal ion incorporation has been employed to enhance the separation of isomeric glycans.					
32070621	6	71	theme	IMS	1064:1066	arg1	separation					1068:1077	partial IMS separation	1056:1077	partial IMS separation	1056:1077	By selecting optimum glycan-metal ion complexes, partial IMS separation was realized for all the 21 isomeric glycan pairs used in the experimental study.					
32070621	3	72	theme	mobility	402:409	arg1	spectrometry					411:422	ion mobility spectrometry	398:422	ion mobility spectrometry	398:422	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	4	73	theme	isomeric	824:831	arg1	glycans					833:839	isomeric glycans	824:839	isomeric glycans	824:839	To mitigate the low IMS resolving power, metal ion incorporation has been employed to enhance the separation of isomeric glycans.					
32070621	6	74	theme	experimental	1141:1152	arg1	study					1154:1158	the experimental study	1137:1158	the experimental study	1137:1158	By selecting optimum glycan-metal ion complexes, partial IMS separation was realized for all the 21 isomeric glycan pairs used in the experimental study.					
32070621	5	75	theme	systematic	861:870	arg1	investigation					872:884	a systematic investigation	859:884	a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans	859:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	5	76	from	investigation	872:884	arg1	attempt					937:943	an attempt to optimize the IMS separation of different isomeric glycans	934:1004	an attempt to optimize the IMS separation of different isomeric glycans	934:1004	Here, we present a systematic investigation of many different glycan-metal ion complexes in an attempt to optimize the IMS separation of different isomeric glycans.					
32070621	3	77	theme	spectrometry	433:444	arg1	combination					383:393	the combination	379:393	the combination of ion mobility spectrometry and mass spectrometry (IMS-MS)	379:453	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	7	78	theme	isomeric	1215:1222	arg1	pairs					1231:1235	these isomeric glycan pairs	1209:1235	these isomeric glycan pairs	1209:1235	Baseline IMS separation was achieved for 76% of these isomeric glycan pairs.					
32070621	0	79	theme	metal	41:45	arg1	incorporation					51:63	metal ion incorporation	41:63	metal ion incorporation for drift tube ion mobility-mass spectrometry	41:109	Improving glycan isomeric separation via metal ion incorporation for drift tube ion mobility-mass spectrometry.					
32070621	6	80	theme	isomeric	1107:1114	arg1	pairs					1123:1127	all the 21 isomeric glycan pairs	1096:1127	all the 21 isomeric glycan pairs used in the experimental study	1096:1158	By selecting optimum glycan-metal ion complexes, partial IMS separation was realized for all the 21 isomeric glycan pairs used in the experimental study.					
32070621	3	81	theme	glycan	491:496	arg1	characterization					508:523	glycan structure characterization	491:523	glycan structure characterization	491:523	Although the combination of ion mobility spectrometry and mass spectrometry (IMS-MS) has become a promising technique in glycan structure characterization and composition identification, the insufficient resolving power of most IMS-MS instruments has limited its utility in performing the comprehensive structure characterization of glycans.					
32070621	2	82	theme	glycans	361:367	arg1	compositions					320:331	extremely broad compositions	304:331	extremely broad compositions	304:331	Understanding their biological functions remains extremely difficult due to the extremely broad compositions and structure variations of glycans.					
32070621	2	82	theme	glycans	361:367	arg1	variations					347:356	structure variations	337:356	structure variations	337:356	Understanding their biological functions remains extremely difficult due to the extremely broad compositions and structure variations of glycans.					
32070621	8	83	theme	complex	1367:1373	arg1	[glycan + Ca + Cl					1375:1391	the complex [glycan + Ca + Cl	1363:1391	the complex [glycan + Ca + Cl	1363:1391	The best IMS separation of isomeric glycans was achieved in some cases by incorporating multiple ions with a glycan, such as the complex [glycan + Ca + Cl]+.					
32070621	9	84	theme	IMS-MS	1424:1429	arg1	trendlines					1443:1452	the well-known IMS-MS measurement trendlines	1409:1452	the well-known IMS-MS measurement trendlines	1409:1452	In addition, the well-known IMS-MS measurement trendlines, often used to identify specific compound classes, were preserved for glycans even for all the 270 glycan-metal ion complexes observed in IMS-MS spectra.					
32070621	0	85	theme	glycan	10:15	arg1	separation					26:35	glycan isomeric separation	10:35	glycan isomeric separation	10:35	Improving glycan isomeric separation via metal ion incorporation for drift tube ion mobility-mass spectrometry.					
32484800	8	0	theme	disease	1630:1636	arg1	phenotype					1638:1646	disease phenotype	1630:1646	disease phenotype	1630:1646	This work suggests a causative relationship between a strain-specific structure, posttranslational modifications, and disease phenotype.					
32484800	5	1	with	isoform	973:979	arg1	signature					1018:1026	a unique sialoglycoform signature	994:1026	a unique sialoglycoform signature	994:1026	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	5	1	with	isoform	973:979	arg1	phenotype					1040:1048	disease phenotype	1032:1048	disease phenotype	1032:1048	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	7	2	gly	sialoglycoforms	1408:1422	arg1	PrPSc					1402:1406	PrPSc sialoglycoforms	1402:1422	PrPSc sialoglycoforms formed as a result of selective recruitment	1402:1466	The current work provides experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes.					
32484800	1	3	theme	neurodegenerative	177:193	arg1	α-synuclein					245:255	α-synuclein	245:255	α-synuclein	245:255	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	1	3	theme	neurodegenerative	177:193	arg1	protein					220:226	prion protein	214:226	prion protein (PrPC)	214:233	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	1	3	theme	neurodegenerative	177:193	arg1	tau					236:238	tau	236:238	tau	236:238	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	1	3	theme	neurodegenerative	177:193	arg1	diseases					195:202	neurodegenerative diseases	177:202	neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein	177:255	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	0	4	theme	phenotype	85:93	arg1	course					39:44	course	39:44	course of prion strain adaptation and disease phenotype	39:93	Posttranslational modifications define course of prion strain adaptation and disease phenotype.					
32484800	4	5	theme	PrPC	604:607	arg1	sialoglycoforms					609:623	PrPC sialoglycoforms	604:623	PrPC sialoglycoforms expressed by a cell	604:643	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	6	6	theme	PrPSc	1162:1166	arg1	features					1229:1236	features	1229:1236	features that are linked to a unique sialoglycoform composition of PrPSc	1229:1300	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	6	6	theme	PrPSc	1162:1166	arg1	response					1219:1226	a very profound proinflammatory response	1187:1226	a very profound proinflammatory response	1187:1226	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	6	6	theme	PrPSc	1162:1166	arg1	colocalization					1144:1157	colocalization	1144:1157	colocalization of PrPSc with microglia	1144:1181	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	5	7	theme	strain	816:821	arg1	transmission					792:803	transmission	792:803	transmission of a prion strain to a new host	792:835	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	7	8	theme	recruitment	1456:1466	arg1	result					1436:1441	a result	1434:1441	a result of selective recruitment	1434:1466	The current work provides experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes.					
32484800	8	9	theme	causative	1533:1541	arg1	relationship					1543:1554	a causative relationship	1531:1554	a causative relationship between a strain-specific structure, posttranslational modifications, and disease phenotype	1531:1646	This work suggests a causative relationship between a strain-specific structure, posttranslational modifications, and disease phenotype.					
32484800	5	10	theme	scrapie	965:971	arg1	isoform					973:979	a self-propagating scrapie isoform	946:979	a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype	946:1048	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	5	10	theme	scrapie	965:971	arg1	PrPSc					982:986	PrPSc	982:986	PrPSc	982:986	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	5	11	theme	disease	1032:1038	arg1	phenotype					1040:1048	disease phenotype	1032:1048	disease phenotype	1032:1048	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	5	12	theme	unique	996:1001	arg1	signature					1018:1026	a unique sialoglycoform signature	994:1026	a unique sialoglycoform signature	994:1026	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	2	13	theme	posttranslational	394:410	arg1	modifications					412:424	posttranslational modifications	394:424	posttranslational modifications	394:424	Alternative self-propagating protein states or strains give rise to different disease phenotypes and display strain-specific subsets of posttranslational modifications.					
32484800	4	14	theme	PrPC	681:684	arg1	molecules					686:694	PrPC molecules	681:694	PrPC molecules	681:694	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	6	15	theme	proinflammatory	1203:1217	arg1	features					1229:1236	features	1229:1236	features that are linked to a unique sialoglycoform composition of PrPSc	1229:1300	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	6	15	theme	proinflammatory	1203:1217	arg1	response					1219:1226	a very profound proinflammatory response	1187:1226	a very profound proinflammatory response	1187:1226	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	7	16	theme	PrPSc	1402:1406	arg1	sialoglycoforms					1408:1422	PrPSc sialoglycoforms	1402:1422	PrPSc sialoglycoforms formed as a result of selective recruitment	1402:1466	The current work provides experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes.					
32484800	3	17	theme	disease	517:523	arg1	phenotype					525:533	disease phenotype	517:533	disease phenotype	517:533	The relationships between strain-specific structure, posttranslational modifications, and disease phenotype are poorly understood.					
32484800	5	18	theme	new	828:830	arg1	host					832:835	a new host	826:835	a new host	826:835	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	0	19	theme	prion	49:53	arg1	adaptation					62:71	prion strain adaptation	49:71	prion strain adaptation	49:71	Posttranslational modifications define course of prion strain adaptation and disease phenotype.					
32484800	0	20	theme	Posttranslational	0:16	arg1	modifications					18:30	Posttranslational modifications	0:30	Posttranslational modifications	0:30	Posttranslational modifications define course of prion strain adaptation and disease phenotype.					
32484800	8	21	theme	posttranslational	1593:1609	arg1	modifications					1611:1623	posttranslational modifications	1593:1623	posttranslational modifications	1593:1623	This work suggests a causative relationship between a strain-specific structure, posttranslational modifications, and disease phenotype.					
32484800	1	22	theme	prion	214:218	arg1	protein					220:226	prion protein	214:226	prion protein (PrPC)	214:233	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	1	22	theme	prion	214:218	arg1	PrPC					229:232	PrPC	229:232	PrPC	229:232	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	7	23	theme	strain-specific	1374:1388	arg1	patterns					1390:1397	strain-specific patterns	1374:1397	strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment	1374:1466	The current work provides experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes.					
32484800	7	24	theme	experimental	1329:1340	arg1	support					1342:1348	experimental support	1329:1348	experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes	1329:1509	The current work provides experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes.					
32484800	1	25	theme	Posttranslational	96:112	arg1	modifications					114:126	Posttranslational modifications	96:126	Posttranslational modifications	96:126	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	1	25	theme	Posttranslational	96:112	arg1	feature					141:147	a common feature	132:147	a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein	132:255	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	7	26	theme	sialoglycoforms	1408:1422	arg1	patterns					1390:1397	strain-specific patterns	1374:1397	strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment	1374:1466	The current work provides experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes.					
32484800	3	27	theme	strain-specific	453:467	arg1	structure					469:477	strain-specific structure	453:477	strain-specific structure	453:477	The relationships between strain-specific structure, posttranslational modifications, and disease phenotype are poorly understood.					
32484800	5	28	gly	sialoglycoforms	914:928	arg1	PrPC					909:912	PrPC sialoglycoforms	909:928	PrPC sialoglycoforms	909:928	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	2	29	theme	disease	336:342	arg1	phenotypes					344:353	different disease phenotypes	326:353	different disease phenotypes	326:353	Alternative self-propagating protein states or strains give rise to different disease phenotypes and display strain-specific subsets of posttranslational modifications.					
32484800	4	30	theme	glycans	763:769	arg1	status					738:743	the sialylation status	722:743	the sialylation status of their N-linked glycans	722:769	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	6	31	theme	sialoglycoform	1266:1279	arg1	composition					1281:1291	a unique sialoglycoform composition	1257:1291	a unique sialoglycoform composition of PrPSc	1257:1300	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	6	32	theme	PrPSc	1296:1300	arg1	composition					1281:1291	a unique sialoglycoform composition	1257:1291	a unique sialoglycoform composition of PrPSc	1257:1300	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	2	33	theme	different	326:334	arg1	phenotypes					344:353	different disease phenotypes	326:353	different disease phenotypes	326:353	Alternative self-propagating protein states or strains give rise to different disease phenotypes and display strain-specific subsets of posttranslational modifications.					
32484800	4	34	theme	sialylation	726:736	arg1	status					738:743	the sialylation status	722:743	the sialylation status of their N-linked glycans	722:769	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	5	35	theme	sialoglycoform	1003:1016	arg1	signature					1018:1026	a unique sialoglycoform signature	994:1026	a unique sialoglycoform signature	994:1026	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	4	36	theme	prion	657:661	arg1	strains					663:669	individual prion strains	646:669	individual prion strains	646:669	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	8	37	theme	strain-specific	1566:1580	arg1	structure					1582:1590	a strain-specific structure	1564:1590	a strain-specific structure	1564:1590	This work suggests a causative relationship between a strain-specific structure, posttranslational modifications, and disease phenotype.					
32484800	6	38	contain	has	1076:1078	arg1	strain					1069:1074	The newly emerged strain	1051:1074	The newly emerged strain	1051:1074	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	6	38	contain	has	1076:1078	arg2	time					1104:1107	the shortest incubation time	1080:1107	the shortest incubation time to disease	1080:1118	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	5	39	theme	PrPC	909:912	arg1	sialoglycoforms					914:928	PrPC sialoglycoforms	909:928	PrPC sialoglycoforms	909:928	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	3	40	theme	posttranslational	480:496	arg1	modifications					498:510	posttranslational modifications	480:510	posttranslational modifications	480:510	The relationships between strain-specific structure, posttranslational modifications, and disease phenotype are poorly understood.					
32484800	4	41	theme	individual	646:655	arg1	strains					663:669	individual prion strains	646:669	individual prion strains	646:669	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	6	42	theme	profound	1194:1201	arg1	features					1229:1236	features	1229:1236	features that are linked to a unique sialoglycoform composition of PrPSc	1229:1300	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	6	42	theme	profound	1194:1201	arg1	response					1219:1226	a very profound proinflammatory response	1187:1226	a very profound proinflammatory response	1187:1226	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	7	43	theme	disease	1492:1498	arg1	phenotypes					1500:1509	strain-specific disease phenotypes	1476:1509	strain-specific disease phenotypes	1476:1509	The current work provides experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes.					
32484800	5	44	theme	sialoglycoforms	914:928	arg1	recruitment					894:904	recruitment	894:904	recruitment of PrPC sialoglycoforms	894:928	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	4	45	theme	N-linked	754:761	arg1	glycans					763:769	their N-linked glycans	748:769	their N-linked glycans	748:769	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	5	46	theme	prion	810:814	arg1	strain					816:821	a prion strain	808:821	a prion strain	808:821	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	7	47	theme	strain-specific	1476:1490	arg1	phenotypes					1500:1509	strain-specific disease phenotypes	1476:1509	strain-specific disease phenotypes	1476:1509	The current work provides experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes.					
32484800	0	48	theme	adaptation	62:71	arg1	course					39:44	course	39:44	course of prion strain adaptation and disease phenotype	39:93	Posttranslational modifications define course of prion strain adaptation and disease phenotype.					
32484800	4	49	theme	sialoglycoforms	609:623	arg1	hundreds					592:599	hundreds	592:599	hundreds of PrPC sialoglycoforms expressed by a cell	592:643	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	5	50	theme	recruitment	894:904	arg1	selectivity					879:889	the selectivity	875:889	the selectivity of recruitment of PrPC sialoglycoforms	875:928	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	6	51	with	colocalization	1144:1157	arg1	microglia					1173:1181	microglia	1173:1181	microglia	1173:1181	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	4	52	link	N-linked	754:761	arg1	glycans					763:769	their N-linked glycans	748:769	their N-linked glycans	748:769	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	1	53	theme	common	134:139	arg1	modifications					114:126	Posttranslational modifications	96:126	Posttranslational modifications	96:126	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	1	53	theme	common	134:139	arg1	feature					141:147	a common feature	132:147	a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein	132:255	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	0	54	theme	strain	55:60	arg1	adaptation					62:71	prion strain adaptation	49:71	prion strain adaptation	49:71	Posttranslational modifications define course of prion strain adaptation and disease phenotype.					
32484800	6	55	attach	linked	1247:1252	arg1	composition					1281:1291	a unique sialoglycoform composition	1257:1291	a unique sialoglycoform composition of PrPSc	1257:1300	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	6	55	attach	linked	1247:1252	arg2	response					1219:1226	a very profound proinflammatory response	1187:1226	a very profound proinflammatory response	1187:1226	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	6	55	attach	linked	1247:1252	arg2	features					1229:1236	features	1229:1236	features that are linked to a unique sialoglycoform composition of PrPSc	1229:1300	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	4	56	gly	sialylation	726:736	arg1	glycans					763:769	their N-linked glycans	748:769	their N-linked glycans	748:769	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	2	57	theme	strain-specific	367:381	arg1	subsets					383:389	strain-specific subsets	367:389	strain-specific subsets of posttranslational modifications	367:424	Alternative self-propagating protein states or strains give rise to different disease phenotypes and display strain-specific subsets of posttranslational modifications.					
32484800	6	58	theme	unique	1259:1264	arg1	composition					1281:1291	a unique sialoglycoform composition	1257:1291	a unique sialoglycoform composition of PrPSc	1257:1300	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	7	59	theme	selective	1446:1454	arg1	recruitment					1456:1466	selective recruitment	1446:1466	selective recruitment	1446:1466	The current work provides experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes.					
32484800	5	60	theme	dramatic	857:864	arg1	shift					866:870	a dramatic shift	855:870	a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms	855:928	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	2	61	theme	protein	287:293	arg1	states					295:300	Alternative self-propagating protein states	258:300	Alternative self-propagating protein states	258:300	Alternative self-propagating protein states or strains give rise to different disease phenotypes and display strain-specific subsets of posttranslational modifications.					
32484800	4	62	gly	sialoglycoforms	609:623	arg1	PrPC					604:607	PrPC sialoglycoforms	604:623	PrPC sialoglycoforms expressed by a cell	604:643	We previously reported that among hundreds of PrPC sialoglycoforms expressed by a cell, individual prion strains recruited PrPC molecules selectively, according to the sialylation status of their N-linked glycans.					
32484800	1	63	theme	proteins	152:159	arg1	modifications					114:126	Posttranslational modifications	96:126	Posttranslational modifications	96:126	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	1	63	theme	proteins	152:159	arg1	feature					141:147	a common feature	132:147	a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein	132:255	Posttranslational modifications are a common feature of proteins associated with neurodegenerative diseases including prion protein (PrPC), tau, and α-synuclein.					
32484800	6	64	theme	incubation	1093:1102	arg1	time					1104:1107	the shortest incubation time	1080:1107	the shortest incubation time to disease	1080:1118	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	0	65	theme	disease	77:83	arg1	phenotype					85:93	disease phenotype	77:93	disease phenotype	77:93	Posttranslational modifications define course of prion strain adaptation and disease phenotype.					
32484800	2	66	theme	self-propagating	270:285	arg1	states					295:300	Alternative self-propagating protein states	258:300	Alternative self-propagating protein states	258:300	Alternative self-propagating protein states or strains give rise to different disease phenotypes and display strain-specific subsets of posttranslational modifications.					
32484800	5	67	theme	self-propagating	948:963	arg1	isoform					973:979	a self-propagating scrapie isoform	946:979	a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype	946:1048	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	5	67	theme	self-propagating	948:963	arg1	PrPSc					982:986	PrPSc	982:986	PrPSc	982:986	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	7	68	theme	current	1307:1313	arg1	work					1315:1318	The current work	1303:1318	The current work	1303:1318	The current work provides experimental support for the hypothesis that strain-specific patterns of PrPSc sialoglycoforms formed as a result of selective recruitment dictate strain-specific disease phenotypes.					
32484800	6	69	theme	shortest	1084:1091	arg1	time					1104:1107	the shortest incubation time	1080:1107	the shortest incubation time to disease	1080:1118	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	6	70	theme	emerged	1061:1067	arg1	strain					1069:1074	The newly emerged strain	1051:1074	The newly emerged strain	1051:1074	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32484800	5	71	from	shift	866:870	arg1	selectivity					879:889	the selectivity	875:889	the selectivity of recruitment of PrPC sialoglycoforms	875:928	Here we report that transmission of a prion strain to a new host is accompanied by a dramatic shift in the selectivity of recruitment of PrPC sialoglycoforms, giving rise to a self-propagating scrapie isoform (PrPSc) with a unique sialoglycoform signature and disease phenotype.					
32484800	2	72	theme	Alternative	258:268	arg1	states					295:300	Alternative self-propagating protein states	258:300	Alternative self-propagating protein states	258:300	Alternative self-propagating protein states or strains give rise to different disease phenotypes and display strain-specific subsets of posttranslational modifications.					
32484800	2	73	theme	modifications	412:424	arg1	subsets					383:389	strain-specific subsets	367:389	strain-specific subsets of posttranslational modifications	367:424	Alternative self-propagating protein states or strains give rise to different disease phenotypes and display strain-specific subsets of posttranslational modifications.					
32484800	6	74	with	response	1219:1226	arg1	microglia					1173:1181	microglia	1173:1181	microglia	1173:1181	The newly emerged strain has the shortest incubation time to disease and is characterized by colocalization of PrPSc with microglia and a very profound proinflammatory response, features that are linked to a unique sialoglycoform composition of PrPSc.					
32857448	4	0	theme	complete	716:723	arg1	unit					725:728	a complete unit	714:728	a complete unit	714:728	The enzymatic product was shown to be successfully incorporated as a complete unit into a tetrasaccharide target by automated assembly.					
32857448	4	0	theme	complete	716:723	arg1	product					661:667	The enzymatic product	647:667	The enzymatic product	647:667	The enzymatic product was shown to be successfully incorporated as a complete unit into a tetrasaccharide target by automated assembly.					
32857448	1	1	theme	protected	266:274	arg1	quantities					243:252	excess quantities	236:252	excess quantities of suitably protected and activated building blocks	236:304	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	1	2	theme	activated	280:288	arg1	blocks					299:304	activated building blocks	280:304	activated building blocks	280:304	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	1	3	theme	Automated	78:86	arg1	concept					140:146	an attractive concept	126:146	an attractive concept that has been successfully applied to a large number of target structures	126:220	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	1	3	theme	Automated	78:86	arg1	synthesis					113:121	Automated chemical oligosaccharide synthesis	78:121	Automated chemical oligosaccharide synthesis	78:121	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	1	4	theme	building	290:297	arg1	blocks					299:304	activated building blocks	280:304	activated building blocks	280:304	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	4	5	theme	tetrasaccharide	737:751	arg1	target					753:758	a tetrasaccharide target	735:758	a tetrasaccharide target by automated assembly	735:780	The enzymatic product was shown to be successfully incorporated as a complete unit into a tetrasaccharide target by automated assembly.					
32857448	1	6	theme	chemical	88:95	arg1	concept					140:146	an attractive concept	126:146	an attractive concept that has been successfully applied to a large number of target structures	126:220	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	1	6	theme	chemical	88:95	arg1	synthesis					113:121	Automated chemical oligosaccharide synthesis	78:121	Automated chemical oligosaccharide synthesis	78:121	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	1	7	theme	large	188:192	arg1	number					194:199	a large number	186:199	a large number of target structures	186:220	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	3	8	theme	NmLgtB-B	427:434	arg1	galactosyltransferase					441:461	the promiscuous NmLgtB-B β1-4 galactosyltransferase	411:461	the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis	411:489	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	3	9	theme	β1-4	436:439	arg1	galactosyltransferase					441:461	the promiscuous NmLgtB-B β1-4 galactosyltransferase	411:461	the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis	411:489	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	1	10	theme	oligosaccharide	97:111	arg1	concept					140:146	an attractive concept	126:146	an attractive concept that has been successfully applied to a large number of target structures	126:220	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	1	10	theme	oligosaccharide	97:111	arg1	synthesis					113:121	Automated chemical oligosaccharide synthesis	78:121	Automated chemical oligosaccharide synthesis	78:121	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	0	11	theme	Building-Block	10:23	arg1	Synthesis					25:33	Enzymatic Building-Block Synthesis	0:33	Enzymatic Building-Block Synthesis	0:33	Enzymatic Building-Block Synthesis for Solid-Phase Automated Glycan Assembly.					
32857448	0	12	theme	Enzymatic	0:8	arg1	Synthesis					25:33	Enzymatic Building-Block Synthesis	0:33	Enzymatic Building-Block Synthesis	0:33	Enzymatic Building-Block Synthesis for Solid-Phase Automated Glycan Assembly.					
32857448	1	13	theme	blocks	299:304	arg1	quantities					243:252	excess quantities	236:252	excess quantities of suitably protected and activated building blocks	236:304	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	4	14	theme	automated	763:771	arg1	assembly					773:780	automated assembly	763:780	automated assembly	763:780	The enzymatic product was shown to be successfully incorporated as a complete unit into a tetrasaccharide target by automated assembly.					
32857448	1	15	theme	target	204:209	arg1	structures					211:220	target structures	204:220	target structures	204:220	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	1	16	theme	structures	211:220	arg1	number					194:199	a large number	186:199	a large number of target structures	186:220	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	3	17	theme	many	560:563	arg1	glycans					578:584	many cell-surface glycans	560:584	many cell-surface glycans	560:584	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	1	18	theme	attractive	129:138	arg1	concept					140:146	an attractive concept	126:146	an attractive concept that has been successfully applied to a large number of target structures	126:220	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	1	18	theme	attractive	129:138	arg1	synthesis					113:121	Automated chemical oligosaccharide synthesis	78:121	Automated chemical oligosaccharide synthesis	78:121	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	3	19	theme	robust	515:520	arg1	access					522:527	fast and robust access	506:527	fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors	506:644	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	0	20	theme	Automated	51:59	arg1	Assembly					68:75	Solid-Phase Automated Glycan Assembly	39:75	Solid-Phase Automated Glycan Assembly	39:75	Enzymatic Building-Block Synthesis for Solid-Phase Automated Glycan Assembly.					
32857448	3	21	theme	LacNAc	536:541	arg1	motif					543:547	the LacNAc motif	532:547	the LacNAc motif	532:547	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	3	22	theme	Neisseria	468:476	arg1	meningitidis					478:489	Neisseria meningitidis	468:489	Neisseria meningitidis	468:489	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	0	23	theme	Solid-Phase	39:49	arg1	Assembly					68:75	Solid-Phase Automated Glycan Assembly	39:75	Solid-Phase Automated Glycan Assembly	39:75	Enzymatic Building-Block Synthesis for Solid-Phase Automated Glycan Assembly.					
32857448	3	24	theme	glycosyl	630:637	arg1	donors					639:644	glycosyl donors	630:644	glycosyl donors	630:644	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	3	25	theme	fast	506:509	arg1	access					522:527	fast and robust access	506:527	fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors	506:644	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	3	26	theme	promiscuous	415:425	arg1	galactosyltransferase					441:461	the promiscuous NmLgtB-B β1-4 galactosyltransferase	411:461	the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis	411:489	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	2	27	theme	such	363:366	arg1	reagents					368:375	supply such reagents	356:375	supply such reagents for automated synthesis	356:399	Herein we demonstrate the use of biocatalysis to supply such reagents for automated synthesis.					
32857448	0	28	theme	Glycan	61:66	arg1	Assembly					68:75	Solid-Phase Automated Glycan Assembly	39:75	Solid-Phase Automated Glycan Assembly	39:75	Enzymatic Building-Block Synthesis for Solid-Phase Automated Glycan Assembly.					
32857448	2	29	theme	supply	356:361	arg1	reagents					368:375	supply such reagents	356:375	supply such reagents for automated synthesis	356:399	Herein we demonstrate the use of biocatalysis to supply such reagents for automated synthesis.					
32857448	1	30	theme	excess	236:241	arg1	quantities					243:252	excess quantities	236:252	excess quantities of suitably protected and activated building blocks	236:304	Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks.					
32857448	3	31	theme	cell-surface	565:576	arg1	glycans					578:584	many cell-surface glycans	560:584	many cell-surface glycans	560:584	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	0	32	dep	Synthesis	25:33	arg1	Assembly					68:75	Solid-Phase Automated Glycan Assembly	39:75	Solid-Phase Automated Glycan Assembly	39:75	Enzymatic Building-Block Synthesis for Solid-Phase Automated Glycan Assembly.					
32857448	2	33	theme	biocatalysis	340:351	arg1	use					333:335	the use	329:335	the use of biocatalysis to supply such reagents for automated synthesis	329:399	Herein we demonstrate the use of biocatalysis to supply such reagents for automated synthesis.					
32857448	3	34	theme	common	550:555	arg1	motif					543:547	the LacNAc motif	532:547	the LacNAc motif	532:547	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	4	35	theme	enzymatic	651:659	arg1	unit					725:728	a complete unit	714:728	a complete unit	714:728	The enzymatic product was shown to be successfully incorporated as a complete unit into a tetrasaccharide target by automated assembly.					
32857448	4	35	theme	enzymatic	651:659	arg1	product					661:667	The enzymatic product	647:667	The enzymatic product	647:667	The enzymatic product was shown to be successfully incorporated as a complete unit into a tetrasaccharide target by automated assembly.					
32857448	3	36	from	meningitidis	478:489	arg1	galactosyltransferase					441:461	the promiscuous NmLgtB-B β1-4 galactosyltransferase	411:461	the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis	411:489	By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors.					
32857448	2	37	theme	automated	381:389	arg1	synthesis					391:399	automated synthesis	381:399	automated synthesis	381:399	Herein we demonstrate the use of biocatalysis to supply such reagents for automated synthesis.					
32289201	2	0	theme	causative	495:503	arg1	agent					505:509	the causative agent	491:509	the causative agent of malaria	491:520	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	2	0	theme	causative	495:503	arg1	infection					448:456	infection	448:456	infection by Plasmodium berghei parasites	448:488	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	4	1	theme	possible	854:861	arg1	transfer					863:870	a possible transfer	852:870	a possible transfer of galactose building blocks from the latter to the former	852:929	Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former.					
32289201	3	2	theme	P.	716:717	arg1	cells					736:740	P. berghei-infected cells	716:740	P. berghei-infected cells	716:740	Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells.					
32289201	1	3	theme	reporter	147:154	arg1	groups					156:161	chemical reporter groups	138:161	chemical reporter groups	138:161	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	5	4	theme	glycan	1079:1084	arg1	structures					1086:1095	key glycan structures	1075:1095	key glycan structures for further vaccine development	1075:1127	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	1	5	theme	bio-orthogonal	250:263	arg1	ligation					265:272	bio-orthogonal ligation	250:272	bio-orthogonal ligation	250:272	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	3	6	theme	compounds	686:694	arg1	uptake					670:675	the uptake	666:675	the uptake of these compounds going from naïve to P. berghei-infected cells	666:740	Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells.					
32289201	1	7	theme	groups	156:161	arg1	introduction					122:133	The introduction	118:133	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation	118:272	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	1	7	theme	groups	156:161	arg1	tool					290:293	an important tool	277:293	an important tool to study glycosylation	277:316	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	5	8	theme	further	1101:1107	arg1	development					1117:1127	further vaccine development	1101:1127	further vaccine development	1101:1127	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	5	9	dep	identification	1036:1049	arg1	the					1032:1034	the	1032:1034	the	1032:1034	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	3	10	theme	GLUT1	573:577	arg1	contribution					557:568	the contribution	553:568	the contribution of GLUT1 to the transport of these galactose derivatives	553:625	Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells.					
32289201	0	11	theme	Study	79:83	arg1	Glycans					85:91	Study Glycans	79:91	Study Glycans in Plasmodium Infection	79:115	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.					
32289201	3	12	theme	berghei-infected	719:734	arg1	cells					736:740	P. berghei-infected cells	716:740	P. berghei-infected cells	716:740	Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells.					
32289201	2	13	theme	derivatives	368:378	arg1	incorporation					331:343	the incorporation	327:343	the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells	327:428	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	0	14	theme	Plasmodium	96:105	arg1	Infection					107:115	Plasmodium Infection	96:115	Plasmodium Infection	96:115	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.					
32289201	5	15	theme	structures	1086:1095	arg1	characterization					1055:1070	characterization	1055:1070	characterization	1055:1070	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	5	15	theme	structures	1086:1095	arg1	identification					1036:1049	identification	1036:1049	identification	1036:1049	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	5	16	theme	key	1075:1077	arg1	structures					1086:1095	key glycan structures	1075:1095	key glycan structures for further vaccine development	1075:1127	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	0	17	theme	Oligosaccharide	38:52	arg1	Engineering					54:64	Metabolic Oligosaccharide Engineering	28:64	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.	0:116	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.					
32289201	1	18	theme	glycan	168:173	arg1	structures					175:184	glycan structures	168:184	glycan structures	168:184	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	0	19	theme	Sweet	2:6	arg1	Transfer					18:25	A Sweet Galactose Transfer	0:25	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.	0:116	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.					
32289201	2	20	contain	bear	385:388	arg1	derivatives					368:378	synthetic galactose derivatives	348:378	synthetic galactose derivatives that bear terminal alkene groups in hepatic cells	348:428	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	2	20	contain	bear	385:388	arg2	groups					406:411	terminal alkene groups	390:411	terminal alkene groups in hepatic cells	390:428	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	5	21	contain	has	946:948	arg1	strategy					937:944	This strategy	932:944	This strategy	932:944	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	5	21	contain	has	946:948	arg2	potential					954:962	the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development	950:1127	the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development	950:1127	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	2	22	theme	alkene	399:404	arg1	groups					406:411	terminal alkene groups	390:411	terminal alkene groups in hepatic cells	390:428	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	1	23	theme	important	280:288	arg1	introduction					122:133	The introduction	118:133	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation	118:272	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	1	23	theme	important	280:288	arg1	tool					290:293	an important tool	277:293	an important tool to study glycosylation	277:316	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	2	24	theme	terminal	390:397	arg1	groups					406:411	terminal alkene groups	390:411	terminal alkene groups in hepatic cells	390:428	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	4	25	theme	Plasmodium	795:804	arg1	parasites					806:814	Plasmodium parasites	795:814	Plasmodium parasites	795:814	Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former.					
32289201	1	26	theme	metabolic	194:202	arg1	MOE					233:235	MOE	233:235	MOE	233:235	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	1	26	theme	metabolic	194:202	arg1	engineering					220:230	metabolic oligosaccharide engineering	194:230	metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation	194:272	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	0	27	theme	Galactose	8:16	arg1	Transfer					18:25	A Sweet Galactose Transfer	0:25	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.	0:116	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.					
32289201	2	28	theme	galactose	358:366	arg1	derivatives					368:378	synthetic galactose derivatives	348:378	synthetic galactose derivatives that bear terminal alkene groups in hepatic cells	348:428	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	1	29	theme	oligosaccharide	204:218	arg1	MOE					233:235	MOE	233:235	MOE	233:235	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	1	29	theme	oligosaccharide	204:218	arg1	engineering					220:230	metabolic oligosaccharide engineering	194:230	metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation	194:272	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
32289201	0	30	theme	Metabolic	28:36	arg1	Engineering					54:64	Metabolic Oligosaccharide Engineering	28:64	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.	0:116	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.					
32289201	5	31	theme	Plasmodium	993:1002	arg1	glycobiology					1004:1015	Plasmodium glycobiology	993:1015	Plasmodium glycobiology	993:1015	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	3	32	from	increase	654:661	arg1	uptake					670:675	the uptake	666:675	the uptake of these compounds going from naïve to P. berghei-infected cells	666:740	Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells.					
32289201	4	33	from	latter	910:915	arg1	transfer					863:870	a possible transfer	852:870	a possible transfer of galactose building blocks from the latter to the former	852:929	Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former.					
32289201	3	34	theme	galactose	605:613	arg1	derivatives					615:625	these galactose derivatives	599:625	these galactose derivatives	599:625	Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells.					
32289201	2	35	theme	malaria	514:520	arg1	agent					505:509	the causative agent	491:509	the causative agent of malaria	491:520	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	2	35	theme	malaria	514:520	arg1	infection					448:456	infection	448:456	infection by Plasmodium berghei parasites	448:488	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	2	36	theme	berghei	472:478	arg1	parasites					480:488	Plasmodium berghei parasites	461:488	Plasmodium berghei parasites	461:488	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	4	37	theme	building	885:892	arg1	blocks					894:899	galactose building blocks	875:899	galactose building blocks	875:899	Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former.					
32289201	3	38	theme	consistent	643:652	arg1	increase					654:661	a consistent increase	641:661	a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells	641:740	Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells.					
32289201	4	39	theme	mosquito	826:833	arg1	hosts					835:839	their mosquito hosts	820:839	their mosquito hosts	820:839	Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former.					
32289201	2	40	theme	synthetic	348:356	arg1	derivatives					368:378	synthetic galactose derivatives	348:378	synthetic galactose derivatives that bear terminal alkene groups in hepatic cells	348:428	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	2	41	theme	Plasmodium	461:470	arg1	parasites					480:488	Plasmodium berghei parasites	461:488	Plasmodium berghei parasites	461:488	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	4	42	theme	galactose	875:883	arg1	blocks					894:899	galactose building blocks	875:899	galactose building blocks	875:899	Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former.					
32289201	0	43	dep	Transfer	18:25	arg1	Engineering					54:64	Metabolic Oligosaccharide Engineering	28:64	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.	0:116	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.					
32289201	2	44	from	groups	406:411	arg1	cells					424:428	hepatic cells	416:428	hepatic cells	416:428	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	4	45	theme	blocks	894:899	arg1	transfer					863:870	a possible transfer	852:870	a possible transfer of galactose building blocks from the latter to the former	852:929	Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former.					
32289201	0	46	from	Glycans	85:91	arg1	Infection					107:115	Plasmodium Infection	96:115	Plasmodium Infection	96:115	A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.					
32289201	5	47	theme	new	975:977	arg1	insights					979:986	new insights	975:986	new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development	975:1127	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	4	48	used	used	755:758	arg2	we					752:753	we	752:753	we	752:753	Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former.					
32289201	2	49	theme	hepatic	416:422	arg1	cells					424:428	hepatic cells	416:428	hepatic cells	416:428	We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria.					
32289201	3	50	theme	derivatives	615:625	arg1	transport					586:594	the transport	582:594	the transport of these galactose derivatives	582:625	Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells.					
32289201	5	51	theme	vaccine	1109:1115	arg1	development					1117:1127	further vaccine development	1101:1127	further vaccine development	1101:1127	This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development.					
32289201	1	52	theme	chemical	138:145	arg1	groups					156:161	chemical reporter groups	138:161	chemical reporter groups	138:161	The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation.					
34687009	0	0	theme	Glycosylation	69:81	arg1	Analysis					83:90	Immunoglobulin (G) Glycosylation Analysis	50:90	Immunoglobulin (G) Glycosylation Analysis	50:90	Capillary (Gel) Electrophoresis-Based Methods for Immunoglobulin (G) Glycosylation Analysis.					
34687009	6	1	theme	detailed	1077:1084	arg1	characterization					1086:1101	the detailed characterization	1073:1101	the detailed characterization of N-glycosylation	1073:1120	The application section describes the detailed characterization of N-glycosylation, utilizing multiplexed CGE with laser-induced fluorescence detection (xCGE-LIF).					
34687009	5	2	with	combination	934:944	arg1	technology					965:974	current C(G)E technology	951:974	current C(G)E technology	951:974	The use of exoglycosidase sequencing in combination with current C(G)E technology is discussed, highlighting possible difficulties and pitfalls.					
34687009	1	3	from	indispensable	159:171	arg1	fields					190:195	many research fields	176:195	many research fields	176:195	The in-depth characterization of protein glycosylation has become indispensable in many research fields and in the biopharmaceutical industry.					
34687009	1	3	from	indispensable	159:171	arg1	industry					226:233	the biopharmaceutical industry	204:233	the biopharmaceutical industry	204:233	The in-depth characterization of protein glycosylation has become indispensable in many research fields and in the biopharmaceutical industry.					
34687009	7	4	theme	human	1301:1305	arg1	immunoglobulins					1307:1321	human immunoglobulins	1301:1321	human immunoglobulins	1301:1321	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	8	5	theme	full	1550:1553	arg1	potential					1555:1563	the full potential	1546:1563	the full potential of C(G)E	1546:1572	These examples illustrate the full potential of C(G)E, resolving the smallest differences in sugar composition and structure.					
34687009	3	6	theme	large	684:688	arg1	studies					697:703	large cohort studies	684:703	large cohort studies	684:703	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	4	7	theme	data	860:863	arg1	analysis					865:872	data analysis	860:872	data analysis	860:872	We focus on the latest developments with respect to miniaturization and mass spectrometry coupling, as well as data analysis and interpretation.					
34687009	3	8	theme	C	548:548	arg1	E					552:552	C(G)E	548:552	C(G)E	548:552	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	2	9	from	effect	327:332	arg1	immunity					337:344	immunity	337:344	immunity	337:344	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	8	10	theme	sugar	1613:1617	arg1	composition					1619:1629	sugar composition	1613:1629	sugar composition	1613:1629	These examples illustrate the full potential of C(G)E, resolving the smallest differences in sugar composition and structure.					
34687009	6	11	theme	fluorescence	1168:1179	arg1	xCGE-LIF					1192:1199	xCGE-LIF	1192:1199	xCGE-LIF	1192:1199	The application section describes the detailed characterization of N-glycosylation, utilizing multiplexed CGE with laser-induced fluorescence detection (xCGE-LIF).					
34687009	6	11	theme	fluorescence	1168:1179	arg1	detection					1181:1189	laser-induced fluorescence detection	1154:1189	laser-induced fluorescence detection (xCGE-LIF)	1154:1200	The application section describes the detailed characterization of N-glycosylation, utilizing multiplexed CGE with laser-induced fluorescence detection (xCGE-LIF).					
34687009	5	12	theme	C	959:959	arg1	technology					965:974	current C(G)E technology	951:974	current C(G)E technology	951:974	The use of exoglycosidase sequencing in combination with current C(G)E technology is discussed, highlighting possible difficulties and pitfalls.					
34687009	3	13	theme	deeper	489:494	arg1	insight					496:502	a deeper insight	487:502	a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis	487:569	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	3	14	dep	electrophoresis-based	525:545	arg1	E					552:552	C(G)E	548:552	C(G)E	548:552	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	3	15	theme	electrophoresis-based	525:545	arg1	analysis					562:569	capillary (gel) electrophoresis-based (C(G)E) glycan analysis	509:569	capillary (gel) electrophoresis-based (C(G)E) glycan analysis	509:569	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	1	16	theme	many	176:179	arg1	fields					190:195	many research fields	176:195	many research fields	176:195	The in-depth characterization of protein glycosylation has become indispensable in many research fields and in the biopharmaceutical industry.					
34687009	4	17	theme	mass	821:824	arg1	spectrometry					826:837	mass spectrometry	821:837	mass spectrometry	821:837	We focus on the latest developments with respect to miniaturization and mass spectrometry coupling, as well as data analysis and interpretation.					
34687009	5	18	theme	G	961:961	arg1	technology					965:974	current C(G)E technology	951:974	current C(G)E technology	951:974	The use of exoglycosidase sequencing in combination with current C(G)E technology is discussed, highlighting possible difficulties and pitfalls.					
34687009	1	19	theme	research	181:188	arg1	fields					190:195	many research fields	176:195	many research fields	176:195	The in-depth characterization of protein glycosylation has become indispensable in many research fields and in the biopharmaceutical industry.					
34687009	5	20	theme	E	963:963	arg1	technology					965:974	current C(G)E technology	951:974	current C(G)E technology	951:974	The use of exoglycosidase sequencing in combination with current C(G)E technology is discussed, highlighting possible difficulties and pitfalls.					
34687009	7	21	theme	species-specific	1275:1290	arg1	G					1332:1332	G	1332:1332	G	1332:1332	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	21	theme	species-specific	1275:1290	arg1	IgGs					1292:1295	species-specific IgGs	1275:1295	species-specific IgGs	1275:1295	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	21	theme	species-specific	1275:1290	arg1	D					1326:1326	D	1326:1326	D	1326:1326	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	21	theme	species-specific	1275:1290	arg1	M					1339:1339	M	1339:1339	M	1339:1339	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	21	theme	species-specific	1275:1290	arg1	E					1329:1329	E	1329:1329	E	1329:1329	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	21	theme	species-specific	1275:1290	arg1	A					1323:1323	A	1323:1323	A	1323:1323	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	5	22	theme	sequencing	920:929	arg1	use					898:900	The use	894:900	The use of exoglycosidase sequencing in combination with current C(G)E technology	894:974	The use of exoglycosidase sequencing in combination with current C(G)E technology is discussed, highlighting possible difficulties and pitfalls.					
34687009	2	23	from	knowledge	247:255	arg1	immunity					337:344	immunity	337:344	immunity	337:344	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	6	24	theme	application	1043:1053	arg1	section					1055:1061	The application section	1039:1061	The application section	1039:1061	The application section describes the detailed characterization of N-glycosylation, utilizing multiplexed CGE with laser-induced fluorescence detection (xCGE-LIF).					
34687009	7	25	theme	cell	1440:1443	arg1	lines					1445:1449	different production cell lines	1419:1449	different production cell lines	1419:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	6	26	theme	laser-induced	1154:1166	arg1	xCGE-LIF					1192:1199	xCGE-LIF	1192:1199	xCGE-LIF	1192:1199	The application section describes the detailed characterization of N-glycosylation, utilizing multiplexed CGE with laser-induced fluorescence detection (xCGE-LIF).					
34687009	6	26	theme	laser-induced	1154:1166	arg1	detection					1181:1189	laser-induced fluorescence detection	1154:1189	laser-induced fluorescence detection (xCGE-LIF)	1154:1200	The application section describes the detailed characterization of N-glycosylation, utilizing multiplexed CGE with laser-induced fluorescence detection (xCGE-LIF).					
34687009	7	27	theme	Fc	1502:1503	arg1	glycosylation					1505:1517	Fc glycosylation	1502:1517	Fc glycosylation	1502:1517	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	8	28	from	differences	1598:1608	arg1	structure					1635:1643	structure	1635:1643	structure	1635:1643	These examples illustrate the full potential of C(G)E, resolving the smallest differences in sugar composition and structure.					
34687009	8	28	from	differences	1598:1608	arg1	composition					1619:1629	sugar composition	1613:1629	sugar composition	1613:1629	These examples illustrate the full potential of C(G)E, resolving the smallest differences in sugar composition and structure.					
34687009	5	29	theme	exoglycosidase	905:918	arg1	sequencing					920:929	exoglycosidase sequencing	905:929	exoglycosidase sequencing	905:929	The use of exoglycosidase sequencing in combination with current C(G)E technology is discussed, highlighting possible difficulties and pitfalls.					
34687009	3	30	theme	G	550:550	arg1	E					552:552	C(G)E	548:552	C(G)E	548:552	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	2	31	from	modulations	263:273	arg1	N-glycosylation					301:315	immunoglobulin G (IgG) N-glycosylation	278:315	immunoglobulin G (IgG) N-glycosylation	278:315	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	5	32	theme	current	951:957	arg1	technology					965:974	current C(G)E technology	951:974	current C(G)E technology	951:974	The use of exoglycosidase sequencing in combination with current C(G)E technology is discussed, highlighting possible difficulties and pitfalls.					
34687009	2	33	theme	drugs	438:442	arg1	development					403:413	the development	399:413	the development of new, more effective drugs for their treatment	399:462	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	2	33	theme	drugs	438:442	arg1	understanding					363:375	a better understanding	354:375	a better understanding of human diseases	354:393	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	7	34	theme	different	1419:1427	arg1	lines					1445:1449	different production cell lines	1419:1449	different production cell lines	1419:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	1	35	theme	in-depth	97:104	arg1	characterization					106:121	The in-depth characterization	93:121	The in-depth characterization of protein glycosylation	93:146	The in-depth characterization of protein glycosylation has become indispensable in many research fields and in the biopharmaceutical industry.					
34687009	7	36	from	comparison	1366:1375	arg1	lines					1445:1449	different production cell lines	1419:1449	different production cell lines	1419:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	4	37	dep	miniaturization	801:815	arg1	coupling					839:846	coupling	839:846	coupling	839:846	We focus on the latest developments with respect to miniaturization and mass spectrometry coupling, as well as data analysis and interpretation.					
34687009	7	38	dep	IgGs	1292:1295	arg1	G					1332:1332	G	1332:1332	G	1332:1332	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	38	dep	IgGs	1292:1295	arg1	IgGs					1292:1295	species-specific IgGs	1275:1295	species-specific IgGs	1275:1295	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	38	dep	IgGs	1292:1295	arg1	D					1326:1326	D	1326:1326	D	1326:1326	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	38	dep	IgGs	1292:1295	arg1	M					1339:1339	M	1339:1339	M	1339:1339	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	38	dep	IgGs	1292:1295	arg1	E					1329:1329	E	1329:1329	E	1329:1329	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	38	dep	IgGs	1292:1295	arg1	A					1323:1323	A	1323:1323	A	1323:1323	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	8	39	theme	G	1570:1570	arg1	E					1572:1572	C(G)E	1568:1572	C(G)E	1568:1572	These examples illustrate the full potential of C(G)E, resolving the smallest differences in sugar composition and structure.					
34687009	3	40	theme	great	633:637	arg1	potential					639:647	its great potential	629:647	its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations	629:746	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	7	41	theme	glycosylation	1505:1517	arg1	characterization					1474:1489	a detailed characterization	1463:1489	a detailed characterization of Fab and Fc glycosylation	1463:1517	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	41	theme	glycosylation	1505:1517	arg1	comparison					1366:1375	a comparison	1364:1375	a comparison of therapeutic monoclonal antibodies from different production cell lines	1364:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	1	42	theme	biopharmaceutical	208:224	arg1	industry					226:233	the biopharmaceutical industry	204:233	the biopharmaceutical industry	204:233	The in-depth characterization of protein glycosylation has become indispensable in many research fields and in the biopharmaceutical industry.					
34687009	0	43	theme	Electrophoresis-Based	16:36	arg1	Methods					38:44	Capillary (Gel) Electrophoresis-Based Methods	0:44	Capillary (Gel) Electrophoresis-Based Methods for Immunoglobulin (G) Glycosylation Analysis.	0:91	Capillary (Gel) Electrophoresis-Based Methods for Immunoglobulin (G) Glycosylation Analysis.					
34687009	7	44	theme	therapeutic	1380:1390	arg1	antibodies					1403:1412	therapeutic monoclonal antibodies	1380:1412	therapeutic monoclonal antibodies from different production cell lines	1380:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	2	45	dep	new	418:420	arg1	effective					428:436	effective	428:436	effective	428:436	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	2	46	theme	new	418:420	arg1	drugs					438:442	new, more effective drugs	418:442	new, more effective drugs for their treatment	418:462	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	0	47	theme	Immunoglobulin	50:63	arg1	Analysis					83:90	Immunoglobulin (G) Glycosylation Analysis	50:90	Immunoglobulin (G) Glycosylation Analysis	50:90	Capillary (Gel) Electrophoresis-Based Methods for Immunoglobulin (G) Glycosylation Analysis.					
34687009	8	48	theme	C	1568:1568	arg1	E					1572:1572	C(G)E	1568:1572	C(G)E	1568:1572	These examples illustrate the full potential of C(G)E, resolving the smallest differences in sugar composition and structure.					
34687009	3	49	theme	glycan	555:560	arg1	analysis					562:569	capillary (gel) electrophoresis-based (C(G)E) glycan analysis	509:569	capillary (gel) electrophoresis-based (C(G)E) glycan analysis	509:569	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	1	50	from	industry	226:233	arg1	indispensable					159:171	indispensable	159:171	indispensable	159:171	The in-depth characterization of protein glycosylation has become indispensable in many research fields and in the biopharmaceutical industry.					
34687009	2	51	theme	better	356:361	arg1	understanding					363:375	a better understanding	354:375	a better understanding of human diseases	354:393	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	7	52	theme	antibodies	1403:1412	arg1	characterization					1474:1489	a detailed characterization	1463:1489	a detailed characterization of Fab and Fc glycosylation	1463:1517	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	52	theme	antibodies	1403:1412	arg1	comparison					1366:1375	a comparison	1364:1375	a comparison of therapeutic monoclonal antibodies from different production cell lines	1364:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	3	53	theme	real	659:662	arg1	high-throughput					664:678	real high-throughput	659:678	real high-throughput for large cohort studies, as well as its challenges and limitations	659:746	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	5	54	theme	possible	1003:1010	arg1	difficulties					1012:1023	possible difficulties	1003:1023	possible difficulties	1003:1023	The use of exoglycosidase sequencing in combination with current C(G)E technology is discussed, highlighting possible difficulties and pitfalls.					
34687009	8	55	theme	smallest	1589:1596	arg1	differences					1598:1608	the smallest differences	1585:1608	the smallest differences in sugar composition and structure	1585:1643	These examples illustrate the full potential of C(G)E, resolving the smallest differences in sugar composition and structure.					
34687009	7	56	theme	detailed	1465:1472	arg1	characterization					1474:1489	a detailed characterization	1463:1489	a detailed characterization of Fab and Fc glycosylation	1463:1517	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	5	57	from	use	898:900	arg1	combination					934:944	combination	934:944	combination with current C(G)E technology	934:974	The use of exoglycosidase sequencing in combination with current C(G)E technology is discussed, highlighting possible difficulties and pitfalls.					
34687009	7	58	from	overview	1227:1234	arg1	glycosylation					1248:1260	antibody glycosylation	1239:1260	antibody glycosylation	1239:1260	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	4	59	theme	latest	765:770	arg1	developments					772:783	the latest developments	761:783	the latest developments with respect to miniaturization and mass spectrometry coupling	761:846	We focus on the latest developments with respect to miniaturization and mass spectrometry coupling, as well as data analysis and interpretation.					
34687009	2	60	theme	G	293:293	arg1	N-glycosylation					301:315	immunoglobulin G (IgG) N-glycosylation	278:315	immunoglobulin G (IgG) N-glycosylation	278:315	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	1	61	theme	protein	126:132	arg1	glycosylation					134:146	protein glycosylation	126:146	protein glycosylation	126:146	The in-depth characterization of protein glycosylation has become indispensable in many research fields and in the biopharmaceutical industry.					
34687009	6	62	theme	N-glycosylation	1106:1120	arg1	characterization					1086:1101	the detailed characterization	1073:1101	the detailed characterization of N-glycosylation	1073:1120	The application section describes the detailed characterization of N-glycosylation, utilizing multiplexed CGE with laser-induced fluorescence detection (xCGE-LIF).					
34687009	7	63	theme	monoclonal	1392:1401	arg1	antibodies					1403:1412	therapeutic monoclonal antibodies	1380:1412	therapeutic monoclonal antibodies from different production cell lines	1380:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	64	theme	production	1429:1438	arg1	lines					1445:1449	different production cell lines	1419:1449	different production cell lines	1419:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	65	theme	Fab	1494:1496	arg1	characterization					1474:1489	a detailed characterization	1463:1489	a detailed characterization of Fab and Fc glycosylation	1463:1517	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	65	theme	Fab	1494:1496	arg1	comparison					1366:1375	a comparison	1364:1375	a comparison of therapeutic monoclonal antibodies from different production cell lines	1364:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	2	66	theme	immunoglobulin	278:291	arg1	IgG					296:298	IgG	296:298	IgG	296:298	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	2	66	theme	immunoglobulin	278:291	arg1	G					293:293	immunoglobulin G	278:293	immunoglobulin G (IgG) N-glycosylation	278:315	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	1	67	from	fields	190:195	arg1	indispensable					159:171	indispensable	159:171	indispensable	159:171	The in-depth characterization of protein glycosylation has become indispensable in many research fields and in the biopharmaceutical industry.					
34687009	1	68	theme	glycosylation	134:146	arg1	characterization					106:121	The in-depth characterization	93:121	The in-depth characterization of protein glycosylation	93:146	The in-depth characterization of protein glycosylation has become indispensable in many research fields and in the biopharmaceutical industry.					
34687009	6	69	theme	multiplexed	1133:1143	arg1	CGE					1145:1147	multiplexed CGE	1133:1147	multiplexed CGE	1133:1147	The application section describes the detailed characterization of N-glycosylation, utilizing multiplexed CGE with laser-induced fluorescence detection (xCGE-LIF).					
34687009	8	70	theme	E	1572:1572	arg1	potential					1555:1563	the full potential	1546:1563	the full potential of C(G)E	1546:1572	These examples illustrate the full potential of C(G)E, resolving the smallest differences in sugar composition and structure.					
34687009	7	71	theme	antibody	1239:1246	arg1	glycosylation					1248:1260	antibody glycosylation	1239:1260	antibody glycosylation	1239:1260	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	72	from	lines	1445:1449	arg1	antibodies					1403:1412	therapeutic monoclonal antibodies	1380:1412	therapeutic monoclonal antibodies from different production cell lines	1380:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	72	from	lines	1445:1449	arg1	characterization					1474:1489	a detailed characterization	1463:1489	a detailed characterization of Fab and Fc glycosylation	1463:1517	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	7	72	from	lines	1445:1449	arg1	comparison					1366:1375	a comparison	1364:1375	a comparison of therapeutic monoclonal antibodies from different production cell lines	1364:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	2	73	theme	diseases	386:393	arg1	development					403:413	the development	399:413	the development of new, more effective drugs for their treatment	399:462	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	2	73	theme	diseases	386:393	arg1	understanding					363:375	a better understanding	354:375	a better understanding of human diseases	354:393	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	7	74	from	characterization	1474:1489	arg1	lines					1445:1449	different production cell lines	1419:1449	different production cell lines	1419:1449	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
34687009	3	75	theme	cohort	690:695	arg1	studies					697:703	large cohort studies	684:703	large cohort studies	684:703	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	3	76	theme	impressive	587:596	arg1	performance					598:608	its impressive performance	583:608	its impressive performance	583:608	This chapter provides a deeper insight into capillary (gel) electrophoresis-based (C(G)E) glycan analysis, addressing its impressive performance and possibilities, its great potential regarding real high-throughput for large cohort studies, as well as its challenges and limitations.					
34687009	2	77	theme	human	380:384	arg1	diseases					386:393	human diseases	380:393	human diseases	380:393	Especially knowledge about modulations in immunoglobulin G (IgG) N-glycosylation and their effect on immunity enabled a better understanding of human diseases and the development of new, more effective drugs for their treatment.					
34687009	7	78	theme	comprehensive	1213:1225	arg1	overview					1227:1234	a comprehensive overview	1211:1234	a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M	1211:1339	Besides a comprehensive overview on antibody glycosylation by comparing species-specific IgGs and human immunoglobulins A, D, E, G, and M, the chapter comprises a comparison of therapeutic monoclonal antibodies from different production cell lines, as well as a detailed characterization of Fab and Fc glycosylation.					
32951418	10	0	theme	powerful	1618:1625	arg1	tool					1627:1630	a powerful tool	1616:1630	a powerful tool for rapid assessment of multivalent glycan binding	1616:1681	Exemplar lectin-binding studies were undertaken to confirm the availability of the glycans for binding and show this is a powerful tool for rapid assessment of multivalent glycan binding.					
32951418	10	0	theme	powerful	1618:1625	arg1	this					1608:1611	this	1608:1611	this	1608:1611	Exemplar lectin-binding studies were undertaken to confirm the availability of the glycans for binding and show this is a powerful tool for rapid assessment of multivalent glycan binding.					
32951418	1	1	theme	nonlinear	191:199	arg1	enhancement					201:211	the nonlinear enhancement	187:211	the nonlinear enhancement associated with the cluster glycoside effect	187:256	Glycomaterials display enhanced binding affinity to carbohydrate-binding proteins due to the nonlinear enhancement associated with the cluster glycoside effect.					
32951418	8	2	theme	centrifugation/washing	1217:1238	arg1	cycles					1240:1245	simple centrifugation/washing cycles	1210:1245	simple centrifugation/washing cycles	1210:1245	Facile post-glycosylation purification is achieved by simple centrifugation/washing cycles to remove excess glycan and polymer.					
32951418	3	3	from	application	439:449	arg1	biosensors					454:463	biosensors	454:463	biosensors	454:463	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	3	3	from	application	439:449	arg1	tools					480:484	diagnostic tools	469:484	diagnostic tools	469:484	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	7	4	theme	ring-closed	1138:1148	arg1	form					1150:1153	the ring-closed form	1134:1153	the ring-closed form	1134:1153	Using an isotope-labeled glycan, the efficiency of this reaction was probed in detail to confirm conjugation, with 25% of end-groups being functionalized, predominantly in the ring-closed form.					
32951418	6	5	theme	terminal	883:890	arg1	groups					902:907	terminal amino-oxy groups	883:907	terminal amino-oxy groups	883:907	Here we report a method to generate glyconanoparticles from unprotected glycans by conjugation to polymer tethers bearing terminal amino-oxy groups, which are then immobilized onto gold nanoparticles.					
32951418	10	6	theme	Exemplar	1496:1503	arg1	studies					1520:1526	Exemplar lectin-binding studies	1496:1526	Exemplar lectin-binding studies	1496:1526	Exemplar lectin-binding studies were undertaken to confirm the availability of the glycans for binding and show this is a powerful tool for rapid assessment of multivalent glycan binding.					
32951418	4	7	theme	simple	637:642	arg1	glycans					644:650	only simple glycans	632:650	only simple glycans	632:650	However, conjugating glycans onto these materials can be challenging, necessitating either multiple protecting group manipulations or the use of only simple glycans.					
32951418	7	8	theme	reaction	1018:1025	arg1	efficiency					999:1008	the efficiency	995:1008	the efficiency of this reaction	995:1025	Using an isotope-labeled glycan, the efficiency of this reaction was probed in detail to confirm conjugation, with 25% of end-groups being functionalized, predominantly in the ring-closed form.					
32951418	9	9	theme	conventional	1464:1475	arg1	methods					1487:1493	more conventional synthetic methods	1459:1493	more conventional synthetic methods	1459:1493	This streamlined synthetic approach may be particularly useful for the preparation of glyconanoparticle libraries using automation, to identify hits to be taken forward using more conventional synthetic methods.					
32951418	4	10	theme	only	632:635	arg1	glycans					644:650	only simple glycans	632:650	only simple glycans	632:650	However, conjugating glycans onto these materials can be challenging, necessitating either multiple protecting group manipulations or the use of only simple glycans.					
32951418	3	11	theme	gold	400:403	arg1	cores					405:409	their highly tunable gold cores	379:409	their highly tunable gold cores (size and shape)	379:426	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	3	11	theme	gold	400:403	arg1	shape					421:425	shape	421:425	shape	421:425	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	3	11	theme	gold	400:403	arg1	size					412:415	size	412:415	size	412:415	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	6	12	theme	polymer	859:865	arg1	tethers					867:873	polymer tethers	859:873	polymer tethers bearing terminal amino-oxy groups, which are then immobilized onto gold nanoparticles	859:959	Here we report a method to generate glyconanoparticles from unprotected glycans by conjugation to polymer tethers bearing terminal amino-oxy groups, which are then immobilized onto gold nanoparticles.					
32951418	3	13	theme	diagnostic	469:478	arg1	tools					480:484	diagnostic tools	469:484	diagnostic tools	469:484	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	8	14	theme	excess	1257:1262	arg1	glycan					1264:1269	excess glycan	1257:1269	excess glycan	1257:1269	Facile post-glycosylation purification is achieved by simple centrifugation/washing cycles to remove excess glycan and polymer.					
32951418	5	15	theme	limited	669:675	arg1	diversity					688:696	limited structural diversity	669:696	limited structural diversity compared to glycoarrays which can include hundreds of glycans	669:758	This results in limited structural diversity compared to glycoarrays which can include hundreds of glycans.					
32951418	9	16	theme	streamlined	1289:1299	arg1	approach					1311:1318	This streamlined synthetic approach	1284:1318	This streamlined synthetic approach	1284:1318	This streamlined synthetic approach may be particularly useful for the preparation of glyconanoparticle libraries using automation, to identify hits to be taken forward using more conventional synthetic methods.					
32951418	10	17	theme	glycan	1668:1673	arg1	binding					1675:1681	multivalent glycan binding	1656:1681	multivalent glycan binding	1656:1681	Exemplar lectin-binding studies were undertaken to confirm the availability of the glycans for binding and show this is a powerful tool for rapid assessment of multivalent glycan binding.					
32951418	0	18	theme	Group	11:15	arg1	Synthesis					22:30	Group Free Synthesis	11:30	Group Free Synthesis of Glyconanoparticles	11:52	Protecting Group Free Synthesis of Glyconanoparticles Using Amino-Oxy-Terminated Polymer Ligands.					
32951418	6	19	theme	gold	942:945	arg1	nanoparticles					947:959	gold nanoparticles	942:959	gold nanoparticles	942:959	Here we report a method to generate glyconanoparticles from unprotected glycans by conjugation to polymer tethers bearing terminal amino-oxy groups, which are then immobilized onto gold nanoparticles.					
32951418	5	20	theme	structural	677:686	arg1	diversity					688:696	limited structural diversity	669:696	limited structural diversity compared to glycoarrays which can include hundreds of glycans	669:758	This results in limited structural diversity compared to glycoarrays which can include hundreds of glycans.					
32951418	9	21	theme	synthetic	1301:1309	arg1	approach					1311:1318	This streamlined synthetic approach	1284:1318	This streamlined synthetic approach	1284:1318	This streamlined synthetic approach may be particularly useful for the preparation of glyconanoparticle libraries using automation, to identify hits to be taken forward using more conventional synthetic methods.					
32951418	8	22	theme	post-glycosylation	1163:1180	arg1	purification					1182:1193	Facile post-glycosylation purification	1156:1193	Facile post-glycosylation purification	1156:1193	Facile post-glycosylation purification is achieved by simple centrifugation/washing cycles to remove excess glycan and polymer.					
32951418	9	23	theme	glyconanoparticle	1370:1386	arg1	libraries					1388:1396	glyconanoparticle libraries	1370:1396	glyconanoparticle libraries using automation	1370:1413	This streamlined synthetic approach may be particularly useful for the preparation of glyconanoparticle libraries using automation, to identify hits to be taken forward using more conventional synthetic methods.					
32951418	1	24	theme	enhanced	121:128	arg1	affinity					138:145	enhanced binding affinity	121:145	enhanced binding affinity to carbohydrate-binding proteins	121:178	Glycomaterials display enhanced binding affinity to carbohydrate-binding proteins due to the nonlinear enhancement associated with the cluster glycoside effect.					
32951418	1	25	theme	cluster	233:239	arg1	effect					251:256	the cluster glycoside effect	229:256	the cluster glycoside effect	229:256	Glycomaterials display enhanced binding affinity to carbohydrate-binding proteins due to the nonlinear enhancement associated with the cluster glycoside effect.					
32951418	4	26	theme	group	598:602	arg1	manipulations					604:616	group manipulations	598:616	group manipulations	598:616	However, conjugating glycans onto these materials can be challenging, necessitating either multiple protecting group manipulations or the use of only simple glycans.					
32951418	5	27	theme	glycans	752:758	arg1	hundreds					740:747	hundreds	740:747	hundreds of glycans	740:758	This results in limited structural diversity compared to glycoarrays which can include hundreds of glycans.					
32951418	1	28	theme	binding	130:136	arg1	affinity					138:145	enhanced binding affinity	121:145	enhanced binding affinity to carbohydrate-binding proteins	121:178	Glycomaterials display enhanced binding affinity to carbohydrate-binding proteins due to the nonlinear enhancement associated with the cluster glycoside effect.					
32951418	10	29	theme	glycans	1579:1585	arg1	availability					1559:1570	the availability	1555:1570	the availability of the glycans for binding	1555:1597	Exemplar lectin-binding studies were undertaken to confirm the availability of the glycans for binding and show this is a powerful tool for rapid assessment of multivalent glycan binding.					
32951418	1	30	theme	glycoside	241:249	arg1	effect					251:256	the cluster glycoside effect	229:256	the cluster glycoside effect	229:256	Glycomaterials display enhanced binding affinity to carbohydrate-binding proteins due to the nonlinear enhancement associated with the cluster glycoside effect.					
32951418	0	31	theme	Free	17:20	arg1	Synthesis					22:30	Group Free Synthesis	11:30	Group Free Synthesis of Glyconanoparticles	11:52	Protecting Group Free Synthesis of Glyconanoparticles Using Amino-Oxy-Terminated Polymer Ligands.					
32951418	10	32	theme	multivalent	1656:1666	arg1	binding					1675:1681	multivalent glycan binding	1656:1681	multivalent glycan binding	1656:1681	Exemplar lectin-binding studies were undertaken to confirm the availability of the glycans for binding and show this is a powerful tool for rapid assessment of multivalent glycan binding.					
32951418	6	33	from	glycans	833:839	arg1	glyconanoparticles					797:814	glyconanoparticles	797:814	glyconanoparticles from unprotected glycans	797:839	Here we report a method to generate glyconanoparticles from unprotected glycans by conjugation to polymer tethers bearing terminal amino-oxy groups, which are then immobilized onto gold nanoparticles.					
32951418	0	34	theme	Glyconanoparticles	35:52	arg1	Synthesis					22:30	Group Free Synthesis	11:30	Group Free Synthesis of Glyconanoparticles	11:52	Protecting Group Free Synthesis of Glyconanoparticles Using Amino-Oxy-Terminated Polymer Ligands.					
32951418	8	35	theme	Facile	1156:1161	arg1	purification					1182:1193	Facile post-glycosylation purification	1156:1193	Facile post-glycosylation purification	1156:1193	Facile post-glycosylation purification is achieved by simple centrifugation/washing cycles to remove excess glycan and polymer.					
32951418	3	36	from	cores	405:409	arg1	biosensors					454:463	biosensors	454:463	biosensors	454:463	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	3	36	from	cores	405:409	arg1	tools					480:484	diagnostic tools	469:484	diagnostic tools	469:484	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	4	37	theme	conjugating	496:506	arg1	glycans					508:514	conjugating glycans	496:514	conjugating glycans onto these materials	496:535	However, conjugating glycans onto these materials can be challenging, necessitating either multiple protecting group manipulations or the use of only simple glycans.					
32951418	6	38	theme	unprotected	821:831	arg1	glycans					833:839	unprotected glycans	821:839	unprotected glycans	821:839	Here we report a method to generate glyconanoparticles from unprotected glycans by conjugation to polymer tethers bearing terminal amino-oxy groups, which are then immobilized onto gold nanoparticles.					
32951418	10	39	theme	binding	1675:1681	arg1	assessment					1642:1651	rapid assessment	1636:1651	rapid assessment of multivalent glycan binding	1636:1681	Exemplar lectin-binding studies were undertaken to confirm the availability of the glycans for binding and show this is a powerful tool for rapid assessment of multivalent glycan binding.					
32951418	0	40	theme	Amino-Oxy-Terminated	60:79	arg1	Ligands					89:95	Amino-Oxy-Terminated Polymer Ligands	60:95	Amino-Oxy-Terminated Polymer Ligands	60:95	Protecting Group Free Synthesis of Glyconanoparticles Using Amino-Oxy-Terminated Polymer Ligands.					
32951418	4	41	dep	challenging	544:554	arg1	either					571:576	either	571:576	either	571:576	However, conjugating glycans onto these materials can be challenging, necessitating either multiple protecting group manipulations or the use of only simple glycans.					
32951418	10	42	theme	rapid	1636:1640	arg1	assessment					1642:1651	rapid assessment	1636:1651	rapid assessment of multivalent glycan binding	1636:1681	Exemplar lectin-binding studies were undertaken to confirm the availability of the glycans for binding and show this is a powerful tool for rapid assessment of multivalent glycan binding.					
32951418	0	43	theme	Polymer	81:87	arg1	Ligands					89:95	Amino-Oxy-Terminated Polymer Ligands	60:95	Amino-Oxy-Terminated Polymer Ligands	60:95	Protecting Group Free Synthesis of Glyconanoparticles Using Amino-Oxy-Terminated Polymer Ligands.					
32951418	9	44	theme	synthetic	1477:1485	arg1	methods					1487:1493	more conventional synthetic methods	1459:1493	more conventional synthetic methods	1459:1493	This streamlined synthetic approach may be particularly useful for the preparation of glyconanoparticle libraries using automation, to identify hits to be taken forward using more conventional synthetic methods.					
32951418	3	45	dep	cores	405:409	arg1	cores					405:409	their highly tunable gold cores	379:409	their highly tunable gold cores (size and shape)	379:426	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	3	45	dep	cores	405:409	arg1	shape					421:425	shape	421:425	shape	421:425	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	3	45	dep	cores	405:409	arg1	size					412:415	size	412:415	size	412:415	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	2	46	theme	Gold	259:262	arg1	nanoparticles					264:276	Gold nanoparticles	259:276	Gold nanoparticles bearing glycans	259:292	Gold nanoparticles bearing glycans have attracted significant interest in particular.					
32951418	1	47	theme	carbohydrate-binding	150:169	arg1	proteins					171:178	carbohydrate-binding proteins	150:178	carbohydrate-binding proteins	150:178	Glycomaterials display enhanced binding affinity to carbohydrate-binding proteins due to the nonlinear enhancement associated with the cluster glycoside effect.					
32951418	10	48	theme	lectin-binding	1505:1518	arg1	studies					1520:1526	Exemplar lectin-binding studies	1496:1526	Exemplar lectin-binding studies	1496:1526	Exemplar lectin-binding studies were undertaken to confirm the availability of the glycans for binding and show this is a powerful tool for rapid assessment of multivalent glycan binding.					
32951418	7	49	theme	end-groups	1084:1093	arg1	end-groups					1084:1093	end-groups	1084:1093	end-groups	1084:1093	Using an isotope-labeled glycan, the efficiency of this reaction was probed in detail to confirm conjugation, with 25% of end-groups being functionalized, predominantly in the ring-closed form.					
32951418	7	49	theme	end-groups	1084:1093	arg1	%					1079:1079	25%	1077:1079	25% of end-groups	1077:1093	Using an isotope-labeled glycan, the efficiency of this reaction was probed in detail to confirm conjugation, with 25% of end-groups being functionalized, predominantly in the ring-closed form.					
32951418	4	50	theme	glycans	644:650	arg1	manipulations					604:616	group manipulations	598:616	group manipulations	598:616	However, conjugating glycans onto these materials can be challenging, necessitating either multiple protecting group manipulations or the use of only simple glycans.					
32951418	4	50	theme	glycans	644:650	arg1	use					625:627	the use	621:627	the use of only simple glycans	621:650	However, conjugating glycans onto these materials can be challenging, necessitating either multiple protecting group manipulations or the use of only simple glycans.					
32951418	8	51	theme	simple	1210:1215	arg1	cycles					1240:1245	simple centrifugation/washing cycles	1210:1245	simple centrifugation/washing cycles	1210:1245	Facile post-glycosylation purification is achieved by simple centrifugation/washing cycles to remove excess glycan and polymer.					
32951418	6	52	theme	amino-oxy	892:900	arg1	groups					902:907	terminal amino-oxy groups	883:907	terminal amino-oxy groups	883:907	Here we report a method to generate glyconanoparticles from unprotected glycans by conjugation to polymer tethers bearing terminal amino-oxy groups, which are then immobilized onto gold nanoparticles.					
32951418	2	53	theme	significant	309:319	arg1	interest					321:328	significant interest	309:328	significant interest	309:328	Gold nanoparticles bearing glycans have attracted significant interest in particular.					
32951418	7	54	theme	isotope-labeled	971:985	arg1	glycan					987:992	an isotope-labeled glycan	968:992	an isotope-labeled glycan	968:992	Using an isotope-labeled glycan, the efficiency of this reaction was probed in detail to confirm conjugation, with 25% of end-groups being functionalized, predominantly in the ring-closed form.					
32951418	3	55	theme	tunable	392:398	arg1	cores					405:409	their highly tunable gold cores	379:409	their highly tunable gold cores (size and shape)	379:426	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	3	55	theme	tunable	392:398	arg1	shape					421:425	shape	421:425	shape	421:425	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	3	55	theme	tunable	392:398	arg1	size					412:415	size	412:415	size	412:415	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	9	56	theme	libraries	1388:1396	arg1	preparation					1355:1365	the preparation	1351:1365	the preparation of glyconanoparticle libraries using automation	1351:1413	This streamlined synthetic approach may be particularly useful for the preparation of glyconanoparticle libraries using automation, to identify hits to be taken forward using more conventional synthetic methods.					
32951418	3	57	from	versatility	366:376	arg1	biosensors					454:463	biosensors	454:463	biosensors	454:463	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
32951418	3	57	from	versatility	366:376	arg1	tools					480:484	diagnostic tools	469:484	diagnostic tools	469:484	This is due to their versatility, their highly tunable gold cores (size and shape), and their application in biosensors and diagnostic tools.					
34633372	10	0	theme	detected	1208:1215	arg1	peaks					1226:1230	the detected N-glycan peaks	1204:1230	the detected N-glycan peaks	1204:1230	An application for data analysis of the detected N-glycan peaks is described in the study.					
34633372	5	1	theme	in-depth	480:487	arg1	necessary					534:542	necessary	534:542	necessary	534:542	Therefore, an in-depth characterization method for the N-glycans is necessary.					
34633372	5	1	theme	in-depth	480:487	arg1	method					506:511	an in-depth characterization method	477:511	an in-depth characterization method for the N-glycans	477:529	Therefore, an in-depth characterization method for the N-glycans is necessary.					
34633372	6	2	theme	quantitative	607:618	arg1	analysis					620:627	qualitative and quantitative analysis	591:627	qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS)	591:776	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	5	3	theme	characterization	489:504	arg1	necessary					534:542	necessary	534:542	necessary	534:542	Therefore, an in-depth characterization method for the N-glycans is necessary.					
34633372	5	3	theme	characterization	489:504	arg1	method					506:511	an in-depth characterization method	477:511	an in-depth characterization method for the N-glycans	477:529	Therefore, an in-depth characterization method for the N-glycans is necessary.					
34633372	11	4	gly	glycoprotein	1295:1306	arg1	glycoprotein					1295:1306	any glycoprotein	1291:1306	any glycoprotein extracted from various species	1291:1337	This protocol can be applied to any glycoprotein extracted from various species.					
34633372	0	5	theme	Spectrometric	112:124	arg1	Detection					126:134	Mass Spectrometric Detection	107:134	Mass Spectrometric Detection	107:134	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.					
34633372	9	6	theme	fluorescence	1105:1116	arg1	detection					1118:1126	fluorescence detection	1105:1126	fluorescence detection	1105:1126	In this approach, N-glycan structures were confirmed by the tandem mass spectrometric analysis, whereas fluorescence detection was used for the quantitative analysis.					
34633372	7	7	theme	facile	829:834	arg1	method					836:841	a facile method	827:841	a facile method	827:841	N-glycans were released from glycoproteins with a facile method and labeled by a procainamide fluorophore tag in the strategy.					
34633372	0	8	theme	Mass	107:110	arg1	Detection					126:134	Mass Spectrometric Detection	107:134	Mass Spectrometric Detection	107:134	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.					
34633372	1	9	located	found	175:179	arg2	Glycosylation					137:149	Glycosylation	137:149	Glycosylation	137:149	Glycosylation is a vital modification found in proteins.					
34633372	1	9	located	found	175:179	arg2	modification					162:173	a vital modification	154:173	a vital modification found in proteins	154:191	Glycosylation is a vital modification found in proteins.					
34633372	1	9	located	found	175:179	arg1	proteins					184:191	proteins	184:191	proteins	184:191	Glycosylation is a vital modification found in proteins.					
34633372	9	10	theme	spectrometric	1073:1085	arg1	analysis					1087:1094	the tandem mass spectrometric analysis	1057:1094	the tandem mass spectrometric analysis	1057:1094	In this approach, N-glycan structures were confirmed by the tandem mass spectrometric analysis, whereas fluorescence detection was used for the quantitative analysis.					
34633372	0	11	with	Chromatography	70:83	arg1	Fluorescence					90:101	Fluorescence	90:101	Fluorescence	90:101	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.					
34633372	0	11	with	Chromatography	70:83	arg1	Detection					126:134	Mass Spectrometric Detection	107:134	Mass Spectrometric Detection	107:134	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.					
34633372	7	12	attach	released	794:801	arg1	glycoproteins					808:820	glycoproteins	808:820	glycoproteins	808:820	N-glycans were released from glycoproteins with a facile method and labeled by a procainamide fluorophore tag in the strategy.					
34633372	7	12	attach	released	794:801	arg2	N-glycans					779:787	N-glycans	779:787	N-glycans	779:787	N-glycans were released from glycoproteins with a facile method and labeled by a procainamide fluorophore tag in the strategy.					
34633372	6	13	theme	liquid	672:677	arg1	chromatography					679:692	hydrophilic interaction liquid chromatography	648:692	hydrophilic interaction liquid chromatography equipped with fluorescence detection	648:729	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	6	14	theme	fluorescence	708:719	arg1	detection					721:729	fluorescence detection	708:729	fluorescence detection	708:729	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	10	15	theme	peaks	1226:1230	arg1	analysis					1192:1199	data analysis	1187:1199	data analysis of the detected N-glycan peaks	1187:1230	An application for data analysis of the detected N-glycan peaks is described in the study.					
34633372	9	16	theme	quantitative	1145:1156	arg1	analysis					1158:1165	the quantitative analysis	1141:1165	the quantitative analysis	1141:1165	In this approach, N-glycan structures were confirmed by the tandem mass spectrometric analysis, whereas fluorescence detection was used for the quantitative analysis.					
34633372	6	17	theme	tandem	735:740	arg1	HILIC-FLD-MS/MS					761:775	HILIC-FLD-MS/MS	761:775	HILIC-FLD-MS/MS	761:775	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	6	17	theme	tandem	735:740	arg1	spectrometry					747:758	tandem mass spectrometry	735:758	tandem mass spectrometry (HILIC-FLD-MS/MS)	735:776	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	0	18	theme	N-glycan	0:7	arg1	Profiling					9:17	N-glycan Profiling	0:17	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.	0:135	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.					
34633372	4	19	theme	glycosylation	442:454	arg1	patterns					456:463	glycosylation patterns	442:463	glycosylation patterns	442:463	The efficacy of these drugs is affected by glycosylation patterns.					
34633372	0	20	theme	Glycoproteins	22:34	arg1	Profiling					9:17	N-glycan Profiling	0:17	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.	0:135	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.					
34633372	6	21	theme	comprehensive	564:576	arg1	approach					578:585	a comprehensive approach	562:585	a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS)	562:776	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	9	22	used	used	1132:1135	arg2	detection					1118:1126	fluorescence detection	1105:1126	fluorescence detection	1105:1126	In this approach, N-glycan structures were confirmed by the tandem mass spectrometric analysis, whereas fluorescence detection was used for the quantitative analysis.					
34633372	9	23	theme	mass	1068:1071	arg1	analysis					1087:1094	the tandem mass spectrometric analysis	1057:1094	the tandem mass spectrometric analysis	1057:1094	In this approach, N-glycan structures were confirmed by the tandem mass spectrometric analysis, whereas fluorescence detection was used for the quantitative analysis.					
34633372	8	24	theme	HILIC-FLD-MS/MS	974:988	arg1	technique					990:998	a HILIC-FLD-MS/MS technique	972:998	a HILIC-FLD-MS/MS technique	972:998	Subsequently, the procainamide labeled N-glycans were analyzed by a HILIC-FLD-MS/MS technique.					
34633372	11	25	theme	various	1323:1329	arg1	species					1331:1337	various species	1323:1337	various species	1323:1337	This protocol can be applied to any glycoprotein extracted from various species.					
34633372	2	26	from	alterations	300:310	arg1	diseases					315:322	diseases	315:322	diseases	315:322	N-glycan profiling of glycoproteins is required to detect novel biomarker candidates and determine glycan alterations in diseases.					
34633372	0	27	theme	Hydrophilic	39:49	arg1	Chromatography					70:83	Hydrophilic Interaction Liquid Chromatography	39:83	Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection	39:134	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.					
34633372	7	28	gly	glycoproteins	808:820	arg1	glycoproteins					808:820	glycoproteins	808:820	glycoproteins	808:820	N-glycans were released from glycoproteins with a facile method and labeled by a procainamide fluorophore tag in the strategy.					
34633372	6	29	theme	interaction	660:670	arg1	chromatography					679:692	hydrophilic interaction liquid chromatography	648:692	hydrophilic interaction liquid chromatography equipped with fluorescence detection	648:729	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	9	30	dep	confirmed	1044:1052	arg1	whereas					1097:1103	whereas	1097:1103	whereas	1097:1103	In this approach, N-glycan structures were confirmed by the tandem mass spectrometric analysis, whereas fluorescence detection was used for the quantitative analysis.					
34633372	2	31	theme	N-glycan	194:201	arg1	profiling					203:211	N-glycan profiling	194:211	N-glycan profiling of glycoproteins	194:228	N-glycan profiling of glycoproteins is required to detect novel biomarker candidates and determine glycan alterations in diseases.					
34633372	2	32	theme	biomarker	258:266	arg1	candidates					268:277	novel biomarker candidates	252:277	novel biomarker candidates	252:277	N-glycan profiling of glycoproteins is required to detect novel biomarker candidates and determine glycan alterations in diseases.					
34633372	0	33	theme	Liquid	63:68	arg1	Chromatography					70:83	Hydrophilic Interaction Liquid Chromatography	39:83	Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection	39:134	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.					
34633372	9	34	theme	tandem	1061:1066	arg1	analysis					1087:1094	the tandem mass spectrometric analysis	1057:1094	the tandem mass spectrometric analysis	1057:1094	In this approach, N-glycan structures were confirmed by the tandem mass spectrometric analysis, whereas fluorescence detection was used for the quantitative analysis.					
34633372	3	35	theme	biopharmaceutical	353:369	arg1	glycoproteins					384:396	glycoproteins	384:396	glycoproteins	384:396	Most commercially available biopharmaceutical proteins are glycoproteins.					
34633372	3	35	theme	biopharmaceutical	353:369	arg1	proteins					371:378	Most commercially available biopharmaceutical proteins	325:378	Most commercially available biopharmaceutical proteins	325:378	Most commercially available biopharmaceutical proteins are glycoproteins.					
34633372	2	36	theme	novel	252:256	arg1	candidates					268:277	novel biomarker candidates	252:277	novel biomarker candidates	252:277	N-glycan profiling of glycoproteins is required to detect novel biomarker candidates and determine glycan alterations in diseases.					
34633372	9	37	theme	N-glycan	1019:1026	arg1	structures					1028:1037	N-glycan structures	1019:1037	N-glycan structures	1019:1037	In this approach, N-glycan structures were confirmed by the tandem mass spectrometric analysis, whereas fluorescence detection was used for the quantitative analysis.					
34633372	0	38	theme	Interaction	51:61	arg1	Chromatography					70:83	Hydrophilic Interaction Liquid Chromatography	39:83	Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection	39:134	N-glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection.					
34633372	4	39	theme	drugs	421:425	arg1	efficacy					403:410	The efficacy	399:410	The efficacy of these drugs	399:425	The efficacy of these drugs is affected by glycosylation patterns.					
34633372	8	40	theme	labeled	937:943	arg1	N-glycans					945:953	the procainamide labeled N-glycans	920:953	the procainamide labeled N-glycans	920:953	Subsequently, the procainamide labeled N-glycans were analyzed by a HILIC-FLD-MS/MS technique.					
34633372	3	41	gly	glycoproteins	384:396	arg1	glycoproteins					384:396	glycoproteins	384:396	glycoproteins	384:396	Most commercially available biopharmaceutical proteins are glycoproteins.					
34633372	3	41	gly	glycoproteins	384:396	arg1	proteins					371:378	Most commercially available biopharmaceutical proteins	325:378	Most commercially available biopharmaceutical proteins	325:378	Most commercially available biopharmaceutical proteins are glycoproteins.					
34633372	10	42	theme	data	1187:1190	arg1	analysis					1192:1199	data analysis	1187:1199	data analysis of the detected N-glycan peaks	1187:1230	An application for data analysis of the detected N-glycan peaks is described in the study.					
34633372	8	43	theme	procainamide	924:935	arg1	N-glycans					945:953	the procainamide labeled N-glycans	920:953	the procainamide labeled N-glycans	920:953	Subsequently, the procainamide labeled N-glycans were analyzed by a HILIC-FLD-MS/MS technique.					
34633372	7	44	theme	procainamide	860:871	arg1	tag					885:887	a procainamide fluorophore tag	858:887	a procainamide fluorophore tag	858:887	N-glycans were released from glycoproteins with a facile method and labeled by a procainamide fluorophore tag in the strategy.					
34633372	3	45	theme	available	343:351	arg1	glycoproteins					384:396	glycoproteins	384:396	glycoproteins	384:396	Most commercially available biopharmaceutical proteins are glycoproteins.					
34633372	3	45	theme	available	343:351	arg1	proteins					371:378	Most commercially available biopharmaceutical proteins	325:378	Most commercially available biopharmaceutical proteins	325:378	Most commercially available biopharmaceutical proteins are glycoproteins.					
34633372	2	46	theme	glycan	293:298	arg1	alterations					300:310	glycan alterations	293:310	glycan alterations in diseases	293:322	N-glycan profiling of glycoproteins is required to detect novel biomarker candidates and determine glycan alterations in diseases.					
34633372	1	47	theme	vital	156:160	arg1	Glycosylation					137:149	Glycosylation	137:149	Glycosylation	137:149	Glycosylation is a vital modification found in proteins.					
34633372	1	47	theme	vital	156:160	arg1	modification					162:173	a vital modification	154:173	a vital modification found in proteins	154:191	Glycosylation is a vital modification found in proteins.					
34633372	2	48	gly	glycoproteins	216:228	arg1	glycoproteins					216:228	glycoproteins	216:228	glycoproteins	216:228	N-glycan profiling of glycoproteins is required to detect novel biomarker candidates and determine glycan alterations in diseases.					
34633372	7	49	theme	fluorophore	873:883	arg1	tag					885:887	a procainamide fluorophore tag	858:887	a procainamide fluorophore tag	858:887	N-glycans were released from glycoproteins with a facile method and labeled by a procainamide fluorophore tag in the strategy.					
34633372	6	50	theme	N-glycans	632:640	arg1	analysis					620:627	qualitative and quantitative analysis	591:627	qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS)	591:776	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	2	51	theme	glycoproteins	216:228	arg1	profiling					203:211	N-glycan profiling	194:211	N-glycan profiling of glycoproteins	194:228	N-glycan profiling of glycoproteins is required to detect novel biomarker candidates and determine glycan alterations in diseases.					
34633372	6	52	theme	hydrophilic	648:658	arg1	chromatography					679:692	hydrophilic interaction liquid chromatography	648:692	hydrophilic interaction liquid chromatography equipped with fluorescence detection	648:729	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	10	53	theme	N-glycan	1217:1224	arg1	peaks					1226:1230	the detected N-glycan peaks	1204:1230	the detected N-glycan peaks	1204:1230	An application for data analysis of the detected N-glycan peaks is described in the study.					
34633372	6	54	theme	mass	742:745	arg1	HILIC-FLD-MS/MS					761:775	HILIC-FLD-MS/MS	761:775	HILIC-FLD-MS/MS	761:775	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	6	54	theme	mass	742:745	arg1	spectrometry					747:758	tandem mass spectrometry	735:758	tandem mass spectrometry (HILIC-FLD-MS/MS)	735:776	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34633372	6	55	theme	qualitative	591:601	arg1	analysis					620:627	qualitative and quantitative analysis	591:627	qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS)	591:776	Here, we present a comprehensive approach for qualitative and quantitative analysis of N-glycans using hydrophilic interaction liquid chromatography equipped with fluorescence detection and tandem mass spectrometry (HILIC-FLD-MS/MS).					
34806714	4	0	theme	proteomic	789:797	arg1	datasets					799:806	bacterial proteomic datasets	779:806	bacterial proteomic datasets	779:806	Traditionally, the manual determination of glycan compositions within bacterial proteomic datasets made this a largely bespoke analysis restricted to field-specific experts.					
34806714	0	1	link	O-linked	101:108	arg1	Glycopeptides					110:122	O-linked Glycopeptides	101:122	Acinetobacter baumannii O-linked Glycopeptides	77:122	The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides.					
34806714	1	2	theme	common	179:184	arg1	glycosylation					133:145	Protein glycosylation	125:145	Protein glycosylation	125:145	Protein glycosylation is increasingly recognized as a common modification within bacterial organisms, contributing to prokaryotic physiology and optimal infectivity of pathogenic species.					
34806714	1	2	theme	common	179:184	arg1	modification					186:197	a common modification	177:197	a common modification within bacterial organisms	177:224	Protein glycosylation is increasingly recognized as a common modification within bacterial organisms, contributing to prokaryotic physiology and optimal infectivity of pathogenic species.					
34806714	1	3	theme	pathogenic	293:302	arg1	species					304:310	pathogenic species	293:310	pathogenic species	293:310	Protein glycosylation is increasingly recognized as a common modification within bacterial organisms, contributing to prokaryotic physiology and optimal infectivity of pathogenic species.					
34806714	0	4	theme	Acinetobacter	77:89	arg1	Identification					59:72	the Identification	55:72	the Identification of Acinetobacter baumannii O-linked Glycopeptides	55:122	The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides.					
34806714	6	5	theme	unique	1043:1048	arg1	modifications					1050:1062	unique modifications	1043:1062	unique modifications observed on peptide sequences	1043:1092	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	7	6	dep	interpretation	1262:1275	arg1	the					1258:1260	the	1258:1260	the	1258:1260	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	1	7	theme	species	304:310	arg1	infectivity					278:288	optimal infectivity	270:288	optimal infectivity	270:288	Protein glycosylation is increasingly recognized as a common modification within bacterial organisms, contributing to prokaryotic physiology and optimal infectivity of pathogenic species.					
34806714	1	7	theme	species	304:310	arg1	physiology					255:264	prokaryotic physiology	243:264	prokaryotic physiology	243:264	Protein glycosylation is increasingly recognized as a common modification within bacterial organisms, contributing to prokaryotic physiology and optimal infectivity of pathogenic species.					
34806714	8	8	gly	glycopeptides	1479:1491	arg2	glycopeptides					1479:1491	glycopeptides	1479:1491	glycopeptides within samples	1479:1506	Using this approach, glycopeptides within samples can rapidly be identified to understand glycosylation differences.					
34806714	3	9	gly	glycopeptide	540:551	arg2	glycopeptide					540:551	rich glycopeptide data	535:556	rich glycopeptide data	535:556	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	0	10	theme	O-linked	101:108	arg1	Glycopeptides					110:122	O-linked Glycopeptides	101:122	Acinetobacter baumannii O-linked Glycopeptides	77:122	The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides.					
34806714	9	11	gly	glycoproteins	1727:1739	arg1	glycoproteins					1727:1739	novel glycoproteins	1721:1739	novel glycoproteins	1721:1739	Using Acinetobacter baumannii as a model, these approaches enable the comparison of glycan compositions between strains and the identification of novel glycoproteins.					
34806714	3	12	theme	prokaryotic	608:618	arg1	species					620:626	prokaryotic species	608:626	prokaryotic species	608:626	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	7	13	theme	compositions	1444:1455	arg1	knowledge					1420:1428	prior knowledge	1414:1428	prior knowledge of the glycan compositions	1414:1455	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	6	14	theme	modifications	1050:1062	arg1	frequency					1030:1038	the frequency	1026:1038	the frequency of unique modifications observed on peptide sequences	1026:1092	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	6	15	located	observed	1064:1071	arg1	sequences					1084:1092	peptide sequences	1076:1092	peptide sequences	1076:1092	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	6	15	located	observed	1064:1071	arg2	modifications					1050:1062	unique modifications	1043:1062	unique modifications observed on peptide sequences	1043:1092	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	3	16	theme	glycans	588:594	arg1	diversity					575:583	diversity	575:583	diversity	575:583	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	3	16	theme	glycans	588:594	arg1	breadth					563:569	breadth	563:569	breadth	563:569	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	5	17	theme	modifications	998:1010	arg1	identification					972:985	the identification	968:985	the identification of unknown modifications	968:1010	Recently, open searching-based approaches have emerged as a powerful alternative for the identification of unknown modifications.					
34806714	4	18	theme	glycan	752:757	arg1	compositions					759:770	glycan compositions	752:770	glycan compositions within bacterial proteomic datasets	752:806	Traditionally, the manual determination of glycan compositions within bacterial proteomic datasets made this a largely bespoke analysis restricted to field-specific experts.					
34806714	10	19	theme	glycoproteomes	1938:1951	arg1	characterization					1897:1912	the characterization	1893:1912	the characterization of these highly diverse glycoproteomes	1893:1951	Taken together, this work demonstrates the versatility of open database-searching techniques for the identification of bacterial glycosylation, making the characterization of these highly diverse glycoproteomes easier than ever before.					
34806714	5	20	theme	open	893:896	arg1	approaches					914:923	open searching-based approaches	893:923	open searching-based approaches	893:923	Recently, open searching-based approaches have emerged as a powerful alternative for the identification of unknown modifications.					
34806714	10	21	theme	diverse	1930:1936	arg1	glycoproteomes					1938:1951	these highly diverse glycoproteomes	1917:1951	these highly diverse glycoproteomes	1917:1951	Taken together, this work demonstrates the versatility of open database-searching techniques for the identification of bacterial glycosylation, making the characterization of these highly diverse glycoproteomes easier than ever before.					
34806714	1	22	theme	bacterial	206:214	arg1	organisms					216:224	bacterial organisms	206:224	bacterial organisms	206:224	Protein glycosylation is increasingly recognized as a common modification within bacterial organisms, contributing to prokaryotic physiology and optimal infectivity of pathogenic species.					
34806714	7	23	theme	searching	1337:1345	arg1	techniques					1347:1356	open searching techniques	1332:1356	open searching techniques	1332:1356	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	7	24	theme	bacterial	1382:1390	arg1	glycopeptides					1392:1404	bacterial glycopeptides	1382:1404	bacterial glycopeptides	1382:1404	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	7	25	theme	streamlined	1233:1243	arg1	workflow					1245:1252	a streamlined workflow	1231:1252	a streamlined workflow for the interpretation and analysis of glycoproteomic data	1231:1311	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	10	26	theme	database-searching	1805:1822	arg1	techniques					1824:1833	open database-searching techniques	1800:1833	open database-searching techniques	1800:1833	Taken together, this work demonstrates the versatility of open database-searching techniques for the identification of bacterial glycosylation, making the characterization of these highly diverse glycoproteomes easier than ever before.					
34806714	3	27	theme	bottom-up	480:488	arg1	proteomics					490:499	bottom-up proteomics	480:499	bottom-up proteomics	480:499	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	10	28	theme	techniques	1824:1833	arg1	versatility					1785:1795	the versatility	1781:1795	the versatility of open database-searching techniques	1781:1833	Taken together, this work demonstrates the versatility of open database-searching techniques for the identification of bacterial glycosylation, making the characterization of these highly diverse glycoproteomes easier than ever before.					
34806714	7	29	used	used	1365:1368	arg2	techniques					1347:1356	open searching techniques	1332:1356	open searching techniques	1332:1356	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	0	30	dep	Acinetobacter	77:89	arg1	Glycopeptides					110:122	O-linked Glycopeptides	101:122	Acinetobacter baumannii O-linked Glycopeptides	77:122	The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides.					
34806714	0	30	dep	Acinetobacter	77:89	arg1	baumannii					91:99	Acinetobacter baumannii O-linked Glycopeptides	77:122	Acinetobacter baumannii O-linked Glycopeptides	77:122	The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides.					
34806714	6	31	theme	common	1149:1154	arg1	glycans					1156:1162	common glycans	1149:1162	common glycans attached to peptides within complex samples	1149:1206	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	2	32	theme	analytical	428:437	arg1	tools					439:443	high-throughput analytical tools	412:443	high-throughput analytical tools to identify these events	412:468	Due to this, there is increasing interest in characterizing bacterial glycosylation and a need for high-throughput analytical tools to identify these events.					
34806714	0	33	theme	Open	19:22	arg1	Approaches					40:49	Open Searching-based Approaches	19:49	Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides	19:122	The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides.					
34806714	2	34	theme	increasing	335:344	arg1	interest					346:353	increasing interest	335:353	increasing interest in characterizing bacterial glycosylation	335:395	Due to this, there is increasing interest in characterizing bacterial glycosylation and a need for high-throughput analytical tools to identify these events.					
34806714	9	35	theme	glycan	1659:1664	arg1	compositions					1666:1677	glycan compositions	1659:1677	glycan compositions	1659:1677	Using Acinetobacter baumannii as a model, these approaches enable the comparison of glycan compositions between strains and the identification of novel glycoproteins.					
34806714	5	36	theme	powerful	943:950	arg1	alternative					952:962	a powerful alternative	941:962	a powerful alternative for the identification of unknown modifications	941:1010	Recently, open searching-based approaches have emerged as a powerful alternative for the identification of unknown modifications.					
34806714	6	37	theme	searching	1100:1108	arg1	techniques					1110:1119	open searching techniques	1095:1119	open searching techniques	1095:1119	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	1	38	theme	Protein	125:131	arg1	glycosylation					133:145	Protein glycosylation	125:145	Protein glycosylation	125:145	Protein glycosylation is increasingly recognized as a common modification within bacterial organisms, contributing to prokaryotic physiology and optimal infectivity of pathogenic species.					
34806714	1	38	theme	Protein	125:131	arg1	modification					186:197	a common modification	177:197	a common modification within bacterial organisms	177:224	Protein glycosylation is increasingly recognized as a common modification within bacterial organisms, contributing to prokaryotic physiology and optimal infectivity of pathogenic species.					
34806714	3	39	located	observed	596:603	arg2	glycans					588:594	glycans	588:594	glycans observed in prokaryotic species	588:626	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	3	39	located	observed	596:603	arg1	species					620:626	prokaryotic species	608:626	prokaryotic species	608:626	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	3	40	theme	rich	535:538	arg1	data					553:556	rich glycopeptide data	535:556	rich glycopeptide data	535:556	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	0	41	theme	Approaches	40:49	arg1	Application					4:14	The Application	0:14	The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides	0:122	The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides.					
34806714	6	42	theme	open	1095:1098	arg1	techniques					1110:1119	open searching techniques	1095:1119	open searching techniques	1095:1119	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	6	43	theme	complex	1192:1198	arg1	samples					1200:1206	complex samples	1192:1206	complex samples	1192:1206	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	3	44	theme	glycosylation	665:677	arg1	events					679:684	bacterial glycosylation events	655:684	bacterial glycosylation events	655:684	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	4	45	theme	compositions	759:770	arg1	determination					735:747	the manual determination	724:747	the manual determination of glycan compositions within bacterial proteomic datasets	724:806	Traditionally, the manual determination of glycan compositions within bacterial proteomic datasets made this a largely bespoke analysis restricted to field-specific experts.					
34806714	1	46	theme	prokaryotic	243:253	arg1	physiology					255:264	prokaryotic physiology	243:264	prokaryotic physiology	243:264	Protein glycosylation is increasingly recognized as a common modification within bacterial organisms, contributing to prokaryotic physiology and optimal infectivity of pathogenic species.					
34806714	0	47	theme	Searching-based	24:38	arg1	Approaches					40:49	Open Searching-based Approaches	19:49	Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides	19:122	The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides.					
34806714	5	48	theme	searching-based	898:912	arg1	approaches					914:923	open searching-based approaches	893:923	open searching-based approaches	893:923	Recently, open searching-based approaches have emerged as a powerful alternative for the identification of unknown modifications.					
34806714	3	49	theme	events	679:684	arg1	identification					637:650	the identification	633:650	the identification of bacterial glycosylation events	633:684	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	6	50	attach	attached	1164:1171	arg2	glycans					1156:1162	common glycans	1149:1162	common glycans attached to peptides within complex samples	1149:1206	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	6	50	attach	attached	1164:1171	arg1	peptides					1176:1183	peptides	1176:1183	peptides within complex samples	1176:1206	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	4	51	theme	manual	728:733	arg1	determination					735:747	the manual determination	724:747	the manual determination of glycan compositions within bacterial proteomic datasets	724:806	Traditionally, the manual determination of glycan compositions within bacterial proteomic datasets made this a largely bespoke analysis restricted to field-specific experts.					
34806714	7	52	theme	glycoproteomic	1293:1306	arg1	data					1308:1311	glycoproteomic data	1293:1311	glycoproteomic data	1293:1311	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	10	53	theme	glycosylation	1871:1883	arg1	identification					1843:1856	the identification	1839:1856	the identification of bacterial glycosylation	1839:1883	Taken together, this work demonstrates the versatility of open database-searching techniques for the identification of bacterial glycosylation, making the characterization of these highly diverse glycoproteomes easier than ever before.					
34806714	10	54	theme	open	1800:1803	arg1	techniques					1824:1833	open database-searching techniques	1800:1833	open database-searching techniques	1800:1833	Taken together, this work demonstrates the versatility of open database-searching techniques for the identification of bacterial glycosylation, making the characterization of these highly diverse glycoproteomes easier than ever before.					
34806714	8	55	theme	glycosylation	1548:1560	arg1	differences					1562:1572	glycosylation differences	1548:1572	glycosylation differences	1548:1572	Using this approach, glycopeptides within samples can rapidly be identified to understand glycosylation differences.					
34806714	7	56	theme	data	1308:1311	arg1	analysis					1281:1288	analysis	1281:1288	analysis	1281:1288	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	7	56	theme	data	1308:1311	arg1	interpretation					1262:1275	interpretation	1262:1275	interpretation	1262:1275	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	6	57	theme	peptide	1076:1082	arg1	sequences					1084:1092	peptide sequences	1076:1092	peptide sequences	1076:1092	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
34806714	3	58	theme	glycopeptide	540:551	arg1	data					553:556	rich glycopeptide data	535:556	rich glycopeptide data	535:556	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	3	59	theme	bacterial	655:663	arg1	events					679:684	bacterial glycosylation events	655:684	bacterial glycosylation events	655:684	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	9	60	theme	compositions	1666:1677	arg1	comparison					1645:1654	the comparison	1641:1654	the comparison of glycan compositions between strains and the identification of novel glycoproteins	1641:1739	Using Acinetobacter baumannii as a model, these approaches enable the comparison of glycan compositions between strains and the identification of novel glycoproteins.					
34806714	4	61	theme	bespoke	828:834	arg1	analysis					836:843	a largely bespoke analysis	818:843	this a largely bespoke analysis	813:843	Traditionally, the manual determination of glycan compositions within bacterial proteomic datasets made this a largely bespoke analysis restricted to field-specific experts.					
34806714	10	62	dep	making	1886:1891	arg1	ever					1965:1968	ever	1965:1968	ever	1965:1968	Taken together, this work demonstrates the versatility of open database-searching techniques for the identification of bacterial glycosylation, making the characterization of these highly diverse glycoproteomes easier than ever before.					
34806714	10	63	theme	bacterial	1861:1869	arg1	glycosylation					1871:1883	bacterial glycosylation	1861:1883	bacterial glycosylation	1861:1883	Taken together, this work demonstrates the versatility of open database-searching techniques for the identification of bacterial glycosylation, making the characterization of these highly diverse glycoproteomes easier than ever before.					
34806714	2	64	dep	tools	439:443	arg1	identify					448:455	identify	448:455	to identify these events	445:468	Due to this, there is increasing interest in characterizing bacterial glycosylation and a need for high-throughput analytical tools to identify these events.					
34806714	9	65	theme	glycoproteins	1727:1739	arg1	identification					1703:1716	the identification	1699:1716	the identification of novel glycoproteins	1699:1739	Using Acinetobacter baumannii as a model, these approaches enable the comparison of glycan compositions between strains and the identification of novel glycoproteins.					
34806714	9	65	theme	glycoproteins	1727:1739	arg1	strains					1687:1693	strains	1687:1693	strains	1687:1693	Using Acinetobacter baumannii as a model, these approaches enable the comparison of glycan compositions between strains and the identification of novel glycoproteins.					
34806714	2	66	theme	bacterial	373:381	arg1	glycosylation					383:395	bacterial glycosylation	373:395	bacterial glycosylation	373:395	Due to this, there is increasing interest in characterizing bacterial glycosylation and a need for high-throughput analytical tools to identify these events.					
34806714	4	67	theme	field-specific	859:872	arg1	experts					874:880	field-specific experts	859:880	field-specific experts	859:880	Traditionally, the manual determination of glycan compositions within bacterial proteomic datasets made this a largely bespoke analysis restricted to field-specific experts.					
34806714	3	68	theme	data	553:556	arg1	generation					521:530	the generation	517:530	the generation of rich glycopeptide data	517:556	Although bottom-up proteomics readily enables the generation of rich glycopeptide data, the breadth and diversity of glycans observed in prokaryotic species make the identification of bacterial glycosylation events extremely challenging.					
34806714	1	69	theme	optimal	270:276	arg1	infectivity					278:288	optimal infectivity	270:288	optimal infectivity	270:288	Protein glycosylation is increasingly recognized as a common modification within bacterial organisms, contributing to prokaryotic physiology and optimal infectivity of pathogenic species.					
34806714	9	70	theme	novel	1721:1725	arg1	glycoproteins					1727:1739	novel glycoproteins	1721:1739	novel glycoproteins	1721:1739	Using Acinetobacter baumannii as a model, these approaches enable the comparison of glycan compositions between strains and the identification of novel glycoproteins.					
34806714	2	71	theme	high-throughput	412:426	arg1	tools					439:443	high-throughput analytical tools	412:443	high-throughput analytical tools to identify these events	412:468	Due to this, there is increasing interest in characterizing bacterial glycosylation and a need for high-throughput analytical tools to identify these events.					
34806714	5	72	theme	unknown	990:996	arg1	modifications					998:1010	unknown modifications	990:1010	unknown modifications	990:1010	Recently, open searching-based approaches have emerged as a powerful alternative for the identification of unknown modifications.					
34806714	7	73	gly	glycopeptides	1392:1404	arg2	glycopeptides					1392:1404	bacterial glycopeptides	1382:1404	bacterial glycopeptides	1382:1404	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	7	74	theme	open	1332:1335	arg1	techniques					1347:1356	open searching techniques	1332:1356	open searching techniques	1332:1356	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	7	75	theme	glycan	1437:1442	arg1	compositions					1444:1455	the glycan compositions	1433:1455	the glycan compositions	1433:1455	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	4	76	theme	bacterial	779:787	arg1	datasets					799:806	bacterial proteomic datasets	779:806	bacterial proteomic datasets	779:806	Traditionally, the manual determination of glycan compositions within bacterial proteomic datasets made this a largely bespoke analysis restricted to field-specific experts.					
34806714	7	77	theme	prior	1414:1418	arg1	knowledge					1420:1428	prior knowledge	1414:1428	prior knowledge of the glycan compositions	1414:1455	This article presents a streamlined workflow for the interpretation and analysis of glycoproteomic data, demonstrating how open searching techniques can be used to identify bacterial glycopeptides without prior knowledge of the glycan compositions.					
34806714	6	78	theme	glycans	1156:1162	arg1	identification					1131:1144	the identification	1127:1144	the identification of common glycans attached to peptides within complex samples	1127:1206	By analyzing the frequency of unique modifications observed on peptide sequences, open searching techniques allow the identification of common glycans attached to peptides within complex samples.					
33758835	5	0	theme	glycosylated	772:783	arg1	spike					785:789	a fully glycosylated spike	764:789	a fully glycosylated spike	764:789	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	6	1	theme	immunogen	942:950	arg1	glycosylation					952:964	recombinant spike-based SARS-CoV-2 immunogen glycosylation	907:964	recombinant spike-based SARS-CoV-2 immunogen glycosylation	907:964	These results suggest that recombinant spike-based SARS-CoV-2 immunogen glycosylation reproducibly recapitulates signatures of viral glycosylation.					
33758835	4	2	theme	structure	714:722	arg1	reporter					694:701	a highly sensitive reporter	675:701	a highly sensitive reporter of protein structure	675:722	We find patterns which are conserved across all samples and this can be associated with site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure.					
33758835	4	2	theme	structure	714:722	arg1	stalling					632:639	site-specific stalling	618:639	site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure	618:722	We find patterns which are conserved across all samples and this can be associated with site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure.					
33758835	5	3	theme	spike	785:789	arg1	simulations					749:759	Molecular dynamics (MD) simulations	725:759	Molecular dynamics (MD) simulations of a fully glycosylated spike	725:789	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	6	4	theme	SARS-CoV-2	931:940	arg1	glycosylation					952:964	recombinant spike-based SARS-CoV-2 immunogen glycosylation	907:964	recombinant spike-based SARS-CoV-2 immunogen glycosylation	907:964	These results suggest that recombinant spike-based SARS-CoV-2 immunogen glycosylation reproducibly recapitulates signatures of viral glycosylation.					
33758835	4	5	theme	protein	706:712	arg1	structure					714:722	protein structure	706:722	protein structure	706:722	We find patterns which are conserved across all samples and this can be associated with site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure.					
33758835	6	6	theme	spike-based	919:929	arg1	glycosylation					952:964	recombinant spike-based SARS-CoV-2 immunogen glycosylation	907:964	recombinant spike-based SARS-CoV-2 immunogen glycosylation	907:964	These results suggest that recombinant spike-based SARS-CoV-2 immunogen glycosylation reproducibly recapitulates signatures of viral glycosylation.					
33758835	2	7	theme	heterogeneity	283:295	arg1	source					273:278	a potential source	261:278	a potential source of heterogeneity between the many different vaccine candidates under investigation	261:361	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	2	7	theme	heterogeneity	283:295	arg1	abundance					192:200	The abundance	188:200	The abundance of N-linked glycans across the SARS-CoV-2 spike protein	188:256	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	6	8	theme	recombinant	907:917	arg1	glycosylation					952:964	recombinant spike-based SARS-CoV-2 immunogen glycosylation	907:964	recombinant spike-based SARS-CoV-2 immunogen glycosylation	907:964	These results suggest that recombinant spike-based SARS-CoV-2 immunogen glycosylation reproducibly recapitulates signatures of viral glycosylation.					
33758835	1	9	theme	central	66:72	arg1	tenet					74:78	A central tenet	64:78	A central tenet in the design of vaccines	64:104	A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity.					
33758835	1	9	theme	central	66:72	arg1	display					113:119	the display	109:119	the display of native-like antigens in the elicitation of protective immunity	109:185	A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity.					
33758835	3	10	theme	infectious	502:511	arg1	protein					521:527	infectious virus S protein	502:527	infectious virus S protein	502:527	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	3	11	gly	glycosylation	389:401	arg1	proteins					435:442	recombinant SARS-CoV-2 spike proteins	406:442	recombinant SARS-CoV-2 spike proteins from five different laboratories	406:475	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	5	12	theme	enzymatic	843:851	arg1	processing					853:862	enzymatic processing	843:862	enzymatic processing of the glycans	843:877	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	2	13	theme	potential	263:271	arg1	source					273:278	a potential source	261:278	a potential source of heterogeneity between the many different vaccine candidates under investigation	261:361	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	2	13	theme	potential	263:271	arg1	abundance					192:200	The abundance	188:200	The abundance of N-linked glycans across the SARS-CoV-2 spike protein	188:256	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	3	14	theme	proteins	435:442	arg1	glycosylation					389:401	the glycosylation	385:401	the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories	385:475	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	5	15	theme	s	799:799	arg1	model					803:807	s a model	799:807	s a model of steric restrictions that shape enzymatic processing of the glycans	799:877	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	1	16	from	display	113:119	arg1	elicitation					152:162	the elicitation	148:162	the elicitation of protective immunity	148:185	A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity.					
33758835	5	17	gly	glycosylated	772:783	arg1	spike					785:789	a fully glycosylated spike	764:789	a fully glycosylated spike	764:789	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	3	18	theme	different	454:462	arg1	laboratories					464:475	five different laboratories	449:475	five different laboratories	449:475	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	0	19	theme	steric	14:19	arg1	control					21:27	Site-specific steric control	0:27	Site-specific steric control of SARS-CoV-2	0:41	Site-specific steric control of SARS-CoV-2 spike glycosylation.					
33758835	3	20	from	glycosylation	389:401	arg1	laboratories					464:475	five different laboratories	449:475	five different laboratories	449:475	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	2	21	theme	many	309:312	arg1	candidates					332:341	the many different vaccine candidates	305:341	the many different vaccine candidates under investigation	305:361	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	1	22	theme	protective	167:176	arg1	immunity					178:185	protective immunity	167:185	protective immunity	167:185	A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity.					
33758835	0	23	theme	Site-specific	0:12	arg1	control					21:27	Site-specific steric control	0:27	Site-specific steric control of SARS-CoV-2	0:41	Site-specific steric control of SARS-CoV-2 spike glycosylation.					
33758835	1	24	theme	immunity	178:185	arg1	elicitation					152:162	the elicitation	148:162	the elicitation of protective immunity	148:185	A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity.					
33758835	5	25	theme	restrictions	819:830	arg1	model					803:807	s a model	799:807	s a model of steric restrictions that shape enzymatic processing of the glycans	799:877	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	5	26	theme	steric	812:817	arg1	restrictions					819:830	steric restrictions	812:830	steric restrictions that shape enzymatic processing of the glycans	812:877	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	4	27	theme	maturation	651:660	arg1	reporter					694:701	a highly sensitive reporter	675:701	a highly sensitive reporter of protein structure	675:722	We find patterns which are conserved across all samples and this can be associated with site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure.					
33758835	4	27	theme	maturation	651:660	arg1	stalling					632:639	site-specific stalling	618:639	site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure	618:722	We find patterns which are conserved across all samples and this can be associated with site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure.					
33758835	6	28	theme	glycosylation	1013:1025	arg1	signatures					993:1002	signatures	993:1002	signatures of viral glycosylation	993:1025	These results suggest that recombinant spike-based SARS-CoV-2 immunogen glycosylation reproducibly recapitulates signatures of viral glycosylation.					
33758835	5	29	theme	Molecular	725:733	arg1	MD					745:746	MD	745:746	MD	745:746	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	5	29	theme	Molecular	725:733	arg1	dynamics					735:742	Molecular dynamics	725:742	Molecular dynamics (MD) simulations of a fully glycosylated spike	725:789	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	1	30	theme	vaccines	97:104	arg1	design					87:92	the design	83:92	the design of vaccines	83:104	A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity.					
33758835	4	31	theme	glycan	644:649	arg1	maturation					651:660	glycan maturation	644:660	glycan maturation	644:660	We find patterns which are conserved across all samples and this can be associated with site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure.					
33758835	3	32	theme	SARS-CoV-2	418:427	arg1	proteins					435:442	recombinant SARS-CoV-2 spike proteins	406:442	recombinant SARS-CoV-2 spike proteins from five different laboratories	406:475	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	4	33	theme	sensitive	684:692	arg1	reporter					694:701	a highly sensitive reporter	675:701	a highly sensitive reporter of protein structure	675:722	We find patterns which are conserved across all samples and this can be associated with site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure.					
33758835	4	33	theme	sensitive	684:692	arg1	stalling					632:639	site-specific stalling	618:639	site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure	618:722	We find patterns which are conserved across all samples and this can be associated with site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure.					
33758835	5	34	theme	dynamics	735:742	arg1	simulations					749:759	Molecular dynamics (MD) simulations	725:759	Molecular dynamics (MD) simulations of a fully glycosylated spike	725:789	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	6	35	theme	viral	1007:1011	arg1	glycosylation					1013:1025	viral glycosylation	1007:1025	viral glycosylation	1007:1025	These results suggest that recombinant spike-based SARS-CoV-2 immunogen glycosylation reproducibly recapitulates signatures of viral glycosylation.					
33758835	0	36	theme	SARS-CoV-2	32:41	arg1	control					21:27	Site-specific steric control	0:27	Site-specific steric control of SARS-CoV-2	0:41	Site-specific steric control of SARS-CoV-2 spike glycosylation.					
33758835	3	37	theme	spike	429:433	arg1	proteins					435:442	recombinant SARS-CoV-2 spike proteins	406:442	recombinant SARS-CoV-2 spike proteins from five different laboratories	406:475	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	3	38	from	laboratories	464:475	arg1	proteins					435:442	recombinant SARS-CoV-2 spike proteins	406:442	recombinant SARS-CoV-2 spike proteins from five different laboratories	406:475	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	3	38	from	laboratories	464:475	arg1	glycosylation					389:401	the glycosylation	385:401	the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories	385:475	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	5	39	theme	a	801:801	arg1	model					803:807	s a model	799:807	s a model of steric restrictions that shape enzymatic processing of the glycans	799:877	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	2	40	theme	glycans	214:220	arg1	source					273:278	a potential source	261:278	a potential source of heterogeneity between the many different vaccine candidates under investigation	261:361	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	2	40	theme	glycans	214:220	arg1	abundance					192:200	The abundance	188:200	The abundance of N-linked glycans across the SARS-CoV-2 spike protein	188:256	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	3	41	theme	virus	513:517	arg1	protein					521:527	infectious virus S protein	502:527	infectious virus S protein	502:527	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	3	42	theme	recombinant	406:416	arg1	proteins					435:442	recombinant SARS-CoV-2 spike proteins	406:442	recombinant SARS-CoV-2 spike proteins from five different laboratories	406:475	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	2	43	theme	N-linked	205:212	arg1	glycans					214:220	N-linked glycans	205:220	N-linked glycans	205:220	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	3	44	theme	S	519:519	arg1	protein					521:527	infectious virus S protein	502:527	infectious virus S protein	502:527	Here, we investigate the glycosylation of recombinant SARS-CoV-2 spike proteins from five different laboratories and compare them against infectious virus S protein.					
33758835	2	45	theme	vaccine	324:330	arg1	candidates					332:341	the many different vaccine candidates	305:341	the many different vaccine candidates under investigation	305:361	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	4	46	theme	site-specific	618:630	arg1	reporter					694:701	a highly sensitive reporter	675:701	a highly sensitive reporter of protein structure	675:722	We find patterns which are conserved across all samples and this can be associated with site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure.					
33758835	4	46	theme	site-specific	618:630	arg1	stalling					632:639	site-specific stalling	618:639	site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure	618:722	We find patterns which are conserved across all samples and this can be associated with site-specific stalling of glycan maturation which act as a highly sensitive reporter of protein structure.					
33758835	2	47	theme	different	314:322	arg1	candidates					332:341	the many different vaccine candidates	305:341	the many different vaccine candidates under investigation	305:361	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	2	48	link	N-linked	205:212	arg1	glycans					214:220	N-linked glycans	205:220	N-linked glycans	205:220	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	2	49	theme	spike	244:248	arg1	protein					250:256	the SARS-CoV-2 spike protein	229:256	the SARS-CoV-2 spike protein	229:256	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	1	50	theme	native-like	124:134	arg1	antigens					136:143	native-like antigens	124:143	native-like antigens	124:143	A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity.					
33758835	5	51	theme	glycans	871:877	arg1	processing					853:862	enzymatic processing	843:862	enzymatic processing of the glycans	843:877	Molecular dynamics (MD) simulations of a fully glycosylated spike support s a model of steric restrictions that shape enzymatic processing of the glycans.					
33758835	2	52	theme	SARS-CoV-2	233:242	arg1	protein					250:256	the SARS-CoV-2 spike protein	229:256	the SARS-CoV-2 spike protein	229:256	The abundance of N-linked glycans across the SARS-CoV-2 spike protein is a potential source of heterogeneity between the many different vaccine candidates under investigation.					
33758835	1	53	theme	antigens	136:143	arg1	display					113:119	the display	109:119	the display of native-like antigens in the elicitation of protective immunity	109:185	A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity.					
33758835	1	53	theme	antigens	136:143	arg1	tenet					74:78	A central tenet	64:78	A central tenet in the design of vaccines	64:104	A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity.					
33758835	1	54	from	tenet	74:78	arg1	design					87:92	the design	83:92	the design of vaccines	83:104	A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity.					
32681153	7	0	theme	sites	1503:1507	arg1	thousands					1474:1482	thousands	1474:1482	thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan	1474:1595	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	6	1	gly	glycopeptides	1204:1216	arg2	glycopeptides					1204:1216	O-GalNAc glycopeptides	1195:1216	O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1195:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	2	theme	mass	1285:1288	arg1	analysis					1303:1310	tandem mass spectrometry analysis	1278:1310	tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1278:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	3	3	theme	biological	516:525	arg1	significance					527:538	the biological significance	512:538	the biological significance of the modification	512:558	O-GalNAcylation is thought to play roles in protein folding, stability, trafficking and protein interactions, and identification of the site-specific O-GalNAc glycoproteome is a crucial step toward understanding the biological significance of the modification.					
32681153	6	4	with	chromatography	1258:1271	arg1	analysis					1303:1310	tandem mass spectrometry analysis	1278:1310	tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1278:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	2	5	theme	protein	152:158	arg1	glycosylation					160:172	protein glycosylation	152:172	protein glycosylation	152:172	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	2	6	theme	O-linked	274:281	arg1	bond					294:297	an O-linked glycosidic bond	271:297	an O-linked glycosidic bond	271:297	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	6	7	theme	liquid	1251:1256	arg1	protease					1237:1244	the OpeRATOR protease	1224:1244	the OpeRATOR protease	1224:1244	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	7	theme	liquid	1251:1256	arg1	chromatography					1258:1271	(5) liquid chromatography	1247:1271	(5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1247:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	3	8	theme	modification	547:558	arg1	significance					527:538	the biological significance	512:538	the biological significance of the modification	512:558	O-GalNAcylation is thought to play roles in protein folding, stability, trafficking and protein interactions, and identification of the site-specific O-GalNAc glycoproteome is a crucial step toward understanding the biological significance of the modification.					
32681153	6	9	gly	glycopeptides	1324:1336	arg2	quantification					1411:1424	(7) quantification	1407:1424	(7) quantification of O-GalNAc glycopeptides	1407:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	9	gly	glycopeptides	1324:1336	arg2	glycopeptides					1324:1336	O-GalNAc glycopeptides	1315:1336	O-GalNAc glycopeptides	1315:1336	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	9	gly	glycopeptides	1324:1336	arg2	identification					1343:1356	(6) identification	1339:1356	(6) identification of O-GalNAc glycopeptides by database search	1339:1401	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	10	theme	peptide	1160:1166	arg1	conjugation					1168:1178	(4) solid-phase peptide conjugation	1144:1178	(4) solid-phase peptide conjugation	1144:1178	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	4	11	theme	analytical	697:706	arg1	challenges					708:717	analytical challenges	697:717	analytical challenges	697:717	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	5	12	theme	sites	903:907	arg1	mapping					851:857	large-scale mapping	839:857	large-scale mapping of site-specific mucin-type O-GalNAcylation sites	839:907	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	7	13	from	thousands	1474:1482	arg1	hundreds					1514:1521	hundreds	1514:1521	hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan	1514:1595	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	4	14	theme	O-GalNAc	665:672	arg1	structures					681:690	complex O-GalNAc glycan structures	657:690	complex O-GalNAc glycan structures	657:690	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	5	15	theme	spectrometry-based	749:766	arg1	method					768:773	a mass spectrometry-based method	742:773	a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites	742:907	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	5	16	theme	site-specific	862:874	arg1	sites					903:907	site-specific mucin-type O-GalNAcylation sites	862:907	site-specific mucin-type O-GalNAcylation sites	862:907	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	6	17	theme	glycopeptides	1438:1450	arg1	glycopeptides					1324:1336	O-GalNAc glycopeptides	1315:1336	O-GalNAc glycopeptides	1315:1336	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	17	theme	glycopeptides	1438:1450	arg1	identification					1343:1356	(6) identification	1339:1356	(6) identification of O-GalNAc glycopeptides by database search	1339:1401	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	17	theme	glycopeptides	1438:1450	arg1	quantification					1411:1424	(7) quantification	1407:1424	(7) quantification of O-GalNAc glycopeptides	1407:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	18	theme	O-GalNAc	1315:1322	arg1	identification					1343:1356	(6) identification	1339:1356	(6) identification of O-GalNAc glycopeptides by database search	1339:1401	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	18	theme	O-GalNAc	1315:1322	arg1	glycopeptides					1324:1336	O-GalNAc glycopeptides	1315:1336	O-GalNAc glycopeptides	1315:1336	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	18	theme	O-GalNAc	1315:1322	arg1	quantification					1411:1424	(7) quantification	1407:1424	(7) quantification of O-GalNAc glycopeptides	1407:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	1	19	theme	modifications	121:133	arg1	one					90:92	one	90:92	one	90:92	Protein glycosylation is one of the most common protein modifications.					
32681153	1	19	theme	modifications	121:133	arg1	modifications					121:133	the most common protein modifications	97:133	the most common protein modifications	97:133	Protein glycosylation is one of the most common protein modifications.					
32681153	7	20	gly	glycoproteins	1526:1538	arg1	glycoproteins					1526:1538	glycoproteins	1526:1538	glycoproteins with information regarding site-specific O-GalNAc glycan	1526:1595	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	6	21	theme	glycopeptides	1204:1216	arg1	release					1184:1190	release	1184:1190	release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1184:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	21	theme	glycopeptides	1204:1216	arg1	enrichment					1115:1124	(3) enrichment	1111:1124	(3) enrichment of glycopeptides	1111:1141	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	21	theme	glycopeptides	1204:1216	arg1	peptides					1101:1108	peptides	1101:1108	peptides	1101:1108	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	21	theme	glycopeptides	1204:1216	arg1	conjugation					1168:1178	(4) solid-phase peptide conjugation	1144:1178	(4) solid-phase peptide conjugation	1144:1178	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	5	22	theme	glycopeptides	805:817	arg1	extraction					782:791	extraction	782:791	extraction of O-linked glycopeptides (EXoO)	782:824	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	6	23	theme	proteins	1032:1039	arg1	extraction					992:1001	: (1) extraction	986:1001	: (1) extraction	986:1001	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	23	theme	proteins	1032:1039	arg1	digestion					1019:1027	proteolytic digestion	1007:1027	proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1007:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	5	24	gly	glycopeptides	805:817	arg2	EXoO					820:823	EXoO	820:823	EXoO	820:823	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	5	24	gly	glycopeptides	805:817	arg2	glycopeptides					805:817	O-linked glycopeptides	796:817	O-linked glycopeptides (EXoO)	796:824	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	6	25	theme	O-GalNAc	1361:1368	arg1	glycopeptides					1370:1382	O-GalNAc glycopeptides	1361:1382	O-GalNAc glycopeptides	1361:1382	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	26	theme	digestion	1019:1027	arg1	stages					977:982	seven stages	971:982	seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	971:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	27	theme	peptides	1101:1108	arg1	peptides					1044:1051	peptides	1044:1051	peptides	1044:1051	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	27	theme	peptides	1101:1108	arg1	de-salting					1087:1096	de-salting	1087:1096	de-salting	1087:1096	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	0	28	theme	Large-scale	0:10	arg1	mapping					26:32	Large-scale site-specific mapping	0:32	Large-scale site-specific mapping of the O-GalNAc	0:48	Large-scale site-specific mapping of the O-GalNAc glycoproteome.					
32681153	5	29	theme	mucin-type	876:885	arg1	sites					903:907	site-specific mucin-type O-GalNAcylation sites	862:907	site-specific mucin-type O-GalNAcylation sites	862:907	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	6	30	theme	detailed	928:935	arg1	protocol					937:944	a detailed protocol	926:944	a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	926:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	1	31	theme	common	106:111	arg1	modifications					121:133	the most common protein modifications	97:133	the most common protein modifications	97:133	Protein glycosylation is one of the most common protein modifications.					
32681153	3	32	theme	O-GalNAc	450:457	arg1	glycoproteome					459:471	the site-specific O-GalNAc glycoproteome	432:471	the site-specific O-GalNAc glycoproteome	432:471	O-GalNAcylation is thought to play roles in protein folding, stability, trafficking and protein interactions, and identification of the site-specific O-GalNAc glycoproteome is a crucial step toward understanding the biological significance of the modification.					
32681153	6	33	gly	glycopeptides	1370:1382	arg2	glycopeptides					1370:1382	O-GalNAc glycopeptides	1361:1382	O-GalNAc glycopeptides	1361:1382	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	3	34	theme	protein	388:394	arg1	interactions					396:407	protein interactions	388:407	protein interactions	388:407	O-GalNAcylation is thought to play roles in protein folding, stability, trafficking and protein interactions, and identification of the site-specific O-GalNAc glycoproteome is a crucial step toward understanding the biological significance of the modification.					
32681153	2	35	theme	glycosylation	160:172	arg1	O-GalNAcylation					177:191	O-GalNAcylation	177:191	O-GalNAcylation	177:191	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	2	35	theme	glycosylation	160:172	arg1	type					144:147	A major type	136:147	A major type of protein glycosylation	136:172	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	6	36	dep	enrichment	1115:1124	arg1	3					1112:1112	3	1112:1112	3	1112:1112	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	5	37	theme	large-scale	839:849	arg1	mapping					851:857	large-scale mapping	839:857	large-scale mapping of site-specific mucin-type O-GalNAcylation sites	839:907	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	2	38	theme	GalNAc-type	203:213	arg1	glycans					215:221	GalNAc-type glycans	203:221	GalNAc-type glycans	203:221	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	3	39	theme	crucial	478:484	arg1	identification					414:427	identification	414:427	identification of the site-specific O-GalNAc glycoproteome	414:471	O-GalNAcylation is thought to play roles in protein folding, stability, trafficking and protein interactions, and identification of the site-specific O-GalNAc glycoproteome is a crucial step toward understanding the biological significance of the modification.					
32681153	3	39	theme	crucial	478:484	arg1	step					486:489	a crucial step	476:489	a crucial step toward understanding the biological significance of the modification	476:558	O-GalNAcylation is thought to play roles in protein folding, stability, trafficking and protein interactions, and identification of the site-specific O-GalNAc glycoproteome is a crucial step toward understanding the biological significance of the modification.					
32681153	4	40	theme	consensus	611:619	arg1	sequon					621:626	consensus sequon	611:626	consensus sequon of O-GalNAcylation sites	611:651	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	3	41	theme	glycoproteome	459:471	arg1	identification					414:427	identification	414:427	identification of the site-specific O-GalNAc glycoproteome	414:471	O-GalNAcylation is thought to play roles in protein folding, stability, trafficking and protein interactions, and identification of the site-specific O-GalNAc glycoproteome is a crucial step toward understanding the biological significance of the modification.					
32681153	3	41	theme	glycoproteome	459:471	arg1	step					486:489	a crucial step	476:489	a crucial step toward understanding the biological significance of the modification	476:558	O-GalNAcylation is thought to play roles in protein folding, stability, trafficking and protein interactions, and identification of the site-specific O-GalNAc glycoproteome is a crucial step toward understanding the biological significance of the modification.					
32681153	4	42	theme	glycan	674:679	arg1	structures					681:690	complex O-GalNAc glycan structures	657:690	complex O-GalNAc glycan structures	657:690	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	7	43	from	hundreds	1514:1521	arg1	thousands					1474:1482	thousands	1474:1482	thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan	1474:1595	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	7	43	from	hundreds	1514:1521	arg1	sites					1503:1507	O-GalNAcylation sites	1487:1507	O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan	1487:1595	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	7	44	theme	O-GalNAcylation	1487:1501	arg1	sites					1503:1507	O-GalNAcylation sites	1487:1507	O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan	1487:1595	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	6	45	theme	spectrometry	1290:1301	arg1	analysis					1303:1310	tandem mass spectrometry analysis	1278:1310	tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1278:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	8	46	theme	basic	1703:1707	arg1	skills					1720:1725	basic proteomics skills	1703:1725	basic proteomics skills	1703:1725	The protocol can be performed by a researcher with basic proteomics skills and takes about 4 d to complete.					
32681153	4	47	theme	methodology	587:597	arg1	absence					600:606	absence	600:606	absence of consensus sequon of O-GalNAcylation sites	600:651	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	4	47	theme	methodology	587:597	arg1	lack					570:573	lack	570:573	lack of suitable methodology	570:597	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	4	47	theme	methodology	587:597	arg1	structures					681:690	complex O-GalNAc glycan structures	657:690	complex O-GalNAc glycan structures	657:690	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	6	48	theme	tandem	1278:1283	arg1	analysis					1303:1310	tandem mass spectrometry analysis	1278:1310	tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1278:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	2	49	theme	glycosidic	283:292	arg1	bond					294:297	an O-linked glycosidic bond	271:297	an O-linked glycosidic bond	271:297	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	6	50	theme	release	1184:1190	arg1	peptides					1044:1051	peptides	1044:1051	peptides	1044:1051	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	50	theme	release	1184:1190	arg1	de-salting					1087:1096	de-salting	1087:1096	de-salting	1087:1096	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	51	theme	conjugation	1168:1178	arg1	peptides					1044:1051	peptides	1044:1051	peptides	1044:1051	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	51	theme	conjugation	1168:1178	arg1	de-salting					1087:1096	de-salting	1087:1096	de-salting	1087:1096	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	2	52	dep	Ser	247:249	arg1	residues					258:265	residues	258:265	residues	258:265	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	2	53	theme	major	138:142	arg1	O-GalNAcylation					177:191	O-GalNAcylation	177:191	O-GalNAcylation	177:191	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	2	53	theme	major	138:142	arg1	type					144:147	A major type	136:147	A major type of protein glycosylation	136:172	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	7	54	with	glycoproteins	1526:1538	arg1	information					1545:1555	information	1545:1555	information regarding site-specific O-GalNAc glycan	1545:1595	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	6	55	theme	proteolytic	1007:1017	arg1	digestion					1019:1027	proteolytic digestion	1007:1027	proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1007:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	56	theme	solid-phase	1148:1158	arg1	conjugation					1168:1178	(4) solid-phase peptide conjugation	1144:1178	(4) solid-phase peptide conjugation	1144:1178	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	5	57	link	O-linked	796:803	arg1	glycopeptides					805:817	O-linked glycopeptides	796:817	O-linked glycopeptides (EXoO)	796:824	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	5	57	link	O-linked	796:803	arg1	EXoO					820:823	EXoO	820:823	EXoO	820:823	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	5	58	theme	O-GalNAcylation	887:901	arg1	sites					903:907	site-specific mucin-type O-GalNAcylation sites	862:907	site-specific mucin-type O-GalNAcylation sites	862:907	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	6	59	theme	extraction	992:1001	arg1	stages					977:982	seven stages	971:982	seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	971:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	60	dep	conjugation	1168:1178	arg1	4					1145:1145	4	1145:1145	4	1145:1145	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	2	61	link	O-linked	274:281	arg1	bond					294:297	an O-linked glycosidic bond	271:297	an O-linked glycosidic bond	271:297	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	2	62	theme	protein	239:245	arg1	Ser					247:249	protein Ser	239:249	protein Ser	239:249	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	4	63	theme	complex	657:663	arg1	structures					681:690	complex O-GalNAc glycan structures	657:690	complex O-GalNAc glycan structures	657:690	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	6	64	theme	glycopeptides	1324:1336	arg1	analysis					1303:1310	tandem mass spectrometry analysis	1278:1310	tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1278:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	1	65	theme	protein	113:119	arg1	modifications					121:133	the most common protein modifications	97:133	the most common protein modifications	97:133	Protein glycosylation is one of the most common protein modifications.					
32681153	7	66	theme	complex	1635:1641	arg1	samples					1643:1649	complex samples	1635:1649	complex samples	1635:1649	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	6	67	dep	extraction	992:1001	arg1	1					989:989	1	989:989	1	989:989	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	68	theme	OpeRATOR	1228:1235	arg1	protease					1237:1244	the OpeRATOR protease	1224:1244	the OpeRATOR protease	1224:1244	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	68	theme	OpeRATOR	1228:1235	arg1	chromatography					1258:1271	(5) liquid chromatography	1247:1271	(5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1247:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	5	69	theme	O-linked	796:803	arg1	glycopeptides					805:817	O-linked glycopeptides	796:817	O-linked glycopeptides (EXoO)	796:824	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	5	69	theme	O-linked	796:803	arg1	EXoO					820:823	EXoO	820:823	EXoO	820:823	We recently developed a mass spectrometry-based method called extraction of O-linked glycopeptides (EXoO) that enables large-scale mapping of site-specific mucin-type O-GalNAcylation sites.					
32681153	6	70	theme	O-GalNAc	1195:1202	arg1	glycopeptides					1204:1216	O-GalNAc glycopeptides	1195:1216	O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1195:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	71	theme	database	1387:1394	arg1	search					1396:1401	database search	1387:1401	database search	1387:1401	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	72	theme	glycopeptides	1370:1382	arg1	glycopeptides					1324:1336	O-GalNAc glycopeptides	1315:1336	O-GalNAc glycopeptides	1315:1336	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	72	theme	glycopeptides	1370:1382	arg1	identification					1343:1356	(6) identification	1339:1356	(6) identification of O-GalNAc glycopeptides by database search	1339:1401	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	72	theme	glycopeptides	1370:1382	arg1	quantification					1411:1424	(7) quantification	1407:1424	(7) quantification of O-GalNAc glycopeptides	1407:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	1	73	theme	Protein	65:71	arg1	glycosylation					73:85	Protein glycosylation	65:85	Protein glycosylation	65:85	Protein glycosylation is one of the most common protein modifications.					
32681153	7	74	theme	O-GalNAc	1581:1588	arg1	glycan					1590:1595	site-specific O-GalNAc glycan	1567:1595	site-specific O-GalNAc glycan	1567:1595	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	4	75	theme	sites	647:651	arg1	sequon					621:626	consensus sequon	611:626	consensus sequon of O-GalNAcylation sites	611:651	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	2	76	attach	attached	227:234	arg2	glycans					215:221	GalNAc-type glycans	203:221	GalNAc-type glycans	203:221	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	2	76	attach	attached	227:234	arg3	O-GalNAcylation					177:191	O-GalNAcylation	177:191	O-GalNAcylation	177:191	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	2	76	attach	attached	227:234	arg1	Thr					254:256	Thr	254:256	Thr	254:256	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	2	76	attach	attached	227:234	arg1	Ser					247:249	protein Ser	239:249	protein Ser	239:249	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	2	76	attach	attached	227:234	arg3	type					144:147	A major type	136:147	A major type of protein glycosylation	136:172	A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond.					
32681153	6	77	dep	guanidination	1069:1081	arg1	2					1055:1055	2	1055:1055	2	1055:1055	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	3	78	theme	site-specific	436:448	arg1	glycoproteome					459:471	the site-specific O-GalNAc glycoproteome	432:471	the site-specific O-GalNAc glycoproteome	432:471	O-GalNAcylation is thought to play roles in protein folding, stability, trafficking and protein interactions, and identification of the site-specific O-GalNAc glycoproteome is a crucial step toward understanding the biological significance of the modification.					
32681153	0	79	theme	site-specific	12:24	arg1	mapping					26:32	Large-scale site-specific mapping	0:32	Large-scale site-specific mapping of the O-GalNAc	0:48	Large-scale site-specific mapping of the O-GalNAc glycoproteome.					
32681153	6	80	gly	glycopeptides	1438:1450	arg2	glycopeptides					1438:1450	O-GalNAc glycopeptides	1429:1450	O-GalNAc glycopeptides	1429:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	81	gly	glycopeptides	1129:1141	arg2	glycopeptides					1129:1141	glycopeptides	1129:1141	glycopeptides	1129:1141	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	82	theme	O-GalNAc	1429:1436	arg1	glycopeptides					1438:1450	O-GalNAc glycopeptides	1429:1450	O-GalNAc glycopeptides	1429:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	4	83	theme	suitable	578:585	arg1	methodology					587:597	suitable methodology	578:597	suitable methodology	578:597	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	8	84	with	researcher	1687:1696	arg1	skills					1720:1725	basic proteomics skills	1703:1725	basic proteomics skills	1703:1725	The protocol can be performed by a researcher with basic proteomics skills and takes about 4 d to complete.					
32681153	0	85	theme	O-GalNAc	41:48	arg1	mapping					26:32	Large-scale site-specific mapping	0:32	Large-scale site-specific mapping of the O-GalNAc	0:48	Large-scale site-specific mapping of the O-GalNAc glycoproteome.					
32681153	7	86	theme	site-specific	1567:1579	arg1	glycan					1590:1595	site-specific O-GalNAc glycan	1567:1595	site-specific O-GalNAc glycan	1567:1595	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	6	87	theme	sequential	1058:1067	arg1	guanidination					1069:1081	(2) sequential guanidination	1054:1081	(2) sequential guanidination	1054:1081	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	87	theme	sequential	1058:1067	arg1	peptides					1044:1051	peptides	1044:1051	peptides	1044:1051	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	3	88	theme	protein	344:350	arg1	folding					352:358	protein folding	344:358	protein folding	344:358	O-GalNAcylation is thought to play roles in protein folding, stability, trafficking and protein interactions, and identification of the site-specific O-GalNAc glycoproteome is a crucial step toward understanding the biological significance of the modification.					
32681153	7	89	theme	glycoproteins	1526:1538	arg1	hundreds					1514:1521	hundreds	1514:1521	hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan	1514:1595	Using this protocol, thousands of O-GalNAcylation sites from hundreds of glycoproteins with information regarding site-specific O-GalNAc glycan can be identified and quantified from complex samples.					
32681153	6	90	theme	glycopeptides	1129:1141	arg1	release					1184:1190	release	1184:1190	release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides	1184:1450	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	90	theme	glycopeptides	1129:1141	arg1	enrichment					1115:1124	(3) enrichment	1111:1124	(3) enrichment of glycopeptides	1111:1141	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	90	theme	glycopeptides	1129:1141	arg1	peptides					1101:1108	peptides	1101:1108	peptides	1101:1108	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	90	theme	glycopeptides	1129:1141	arg1	conjugation					1168:1178	(4) solid-phase peptide conjugation	1144:1178	(4) solid-phase peptide conjugation	1144:1178	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	4	91	theme	sequon	621:626	arg1	absence					600:606	absence	600:606	absence of consensus sequon of O-GalNAcylation sites	600:651	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	4	91	theme	sequon	621:626	arg1	lack					570:573	lack	570:573	lack of suitable methodology	570:597	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	4	91	theme	sequon	621:626	arg1	structures					681:690	complex O-GalNAc glycan structures	657:690	complex O-GalNAc glycan structures	657:690	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	6	92	theme	enrichment	1115:1124	arg1	peptides					1044:1051	peptides	1044:1051	peptides	1044:1051	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	6	92	theme	enrichment	1115:1124	arg1	de-salting					1087:1096	de-salting	1087:1096	de-salting	1087:1096	Here we provide a detailed protocol for EXoO, which includes seven stages of: (1) extraction and proteolytic digestion of proteins to peptides, (2) sequential guanidination and de-salting of peptides, (3) enrichment of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using the OpeRATOR protease, (5) liquid chromatography with tandem mass spectrometry analysis of O-GalNAc glycopeptides, (6) identification of O-GalNAc glycopeptides by database search and (7) quantification of O-GalNAc glycopeptides.					
32681153	4	93	theme	O-GalNAcylation	631:645	arg1	sites					647:651	O-GalNAcylation sites	631:651	O-GalNAcylation sites	631:651	However, lack of suitable methodology, absence of consensus sequon of O-GalNAcylation sites and complex O-GalNAc glycan structures pose analytical challenges.					
32681153	8	94	theme	proteomics	1709:1718	arg1	skills					1720:1725	basic proteomics skills	1703:1725	basic proteomics skills	1703:1725	The protocol can be performed by a researcher with basic proteomics skills and takes about 4 d to complete.					
33835347	6	0	theme	N-linked	873:880	arg1	engine					911:916	complete N-linked glycopeptide database search engine	864:916	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	2	1	from	regions	386:392	arg1	causes					348:353	the main causes	339:353	the main causes of death in some countries and regions	339:392	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	7	2	theme	monosaccharide	1260:1273	arg1	compositions					1275:1286	228 N-glycan monosaccharide compositions 1026 N-glycan putative structures	1247:1320	228 N-glycan monosaccharide compositions 1026 N-glycan putative structures	1247:1320	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	5	3	theme	continuous	597:606	arg1	research					617:624	the continuous in-depth research	593:624	the continuous in-depth research of glycoproteomics in precision medical diagnosis	593:674	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	6	4	theme	developed	854:862	arg1	GPSeeker					918:925	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	4	5	theme	most	544:547	arg1	cases					549:553	most cases	544:553	most cases	544:553	Therefore, most cases are diagnosed at advanced stage.					
33835347	3	6	from	lack	474:477	arg1	stages					504:509	the early stages	494:509	the early stages of pancreatic cancer	494:530	Currently, pancreatic cancer generally has a poor prognosis, partly due to the lack of symptoms in the early stages of pancreatic cancer.					
33835347	3	7	theme	early	498:502	arg1	stages					504:509	the early stages	494:509	the early stages of pancreatic cancer	494:530	Currently, pancreatic cancer generally has a poor prognosis, partly due to the lack of symptoms in the early stages of pancreatic cancer.					
33835347	5	8	from	diagnosis	666:674	arg1	research					617:624	the continuous in-depth research	593:624	the continuous in-depth research of glycoproteomics in precision medical diagnosis	593:674	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	5	9	theme	tissues	766:772	arg1	analysis					722:729	quantitative analysis	709:729	quantitative analysis of cancer-related cells, plasma or tissues	709:772	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	0	10	theme	cancer	158:163	arg1	study					138:142	a case study	131:142	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.	0:164	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	2	11	theme	developed	297:305	arg1	countries					307:315	developed countries	297:315	developed countries	297:315	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	1	12	theme	malignant	196:204	arg1	difficult					243:251	difficult	243:251	difficult	243:251	Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat.					
33835347	1	12	theme	malignant	196:204	arg1	tumor					206:210	a highly malignant tumor	187:210	a highly malignant tumor of the digestive tract that is difficult to diagnose and treat	187:273	Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat.					
33835347	1	12	theme	malignant	196:204	arg1	cancer					177:182	Pancreatic cancer	166:182	Pancreatic cancer	166:182	Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat.					
33835347	0	13	theme	case	133:136	arg1	study					138:142	a case study	131:142	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.	0:164	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	1	14	theme	digestive	219:227	arg1	tract					229:233	the digestive tract	215:233	the digestive tract	215:233	Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat.					
33835347	6	15	theme	engine	911:916	arg1	GPSeeker					918:925	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	5	16	theme	glycoproteomics	629:643	arg1	research					617:624	the continuous in-depth research	593:624	the continuous in-depth research of glycoproteomics in precision medical diagnosis	593:674	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	6	17	theme	database	895:902	arg1	engine					911:916	complete N-linked glycopeptide database search engine	864:916	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	8	18	theme	cancer	1530:1535	arg1	tussues					1537:1543	pancreatic cancer tussues	1519:1543	pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively	1519:1622	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	7	19	theme	spectrum-level	1152:1165	arg1	%					1173:1173	spectrum-level FDR ≤1%	1152:1173	spectrum-level FDR ≤1%	1152:1173	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	5	20	theme	precision	648:656	arg1	diagnosis					666:674	precision medical diagnosis	648:674	precision medical diagnosis	648:674	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	8	21	theme	relative	1545:1552	arg1	tussues					1537:1543	pancreatic cancer tussues	1519:1543	pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively	1519:1622	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	7	22	theme	228 N-glycan	1247:1258	arg1	compositions					1275:1286	228 N-glycan monosaccharide compositions 1026 N-glycan putative structures	1247:1320	228 N-glycan monosaccharide compositions 1026 N-glycan putative structures	1247:1320	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	6	23	theme	isotope	986:992	arg1	labeling					994:1001	stable diethyl isotope labeling	971:1001	stable diethyl isotope labeling	971:1001	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	6	24	gly	glycopeptide	882:893	arg2	glycopeptide					882:893	complete N-linked glycopeptide database search engine	864:916	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	7	25	gly	N-glycoproteins	1357:1371	arg1	N-glycoproteins					1357:1371	3437 intact N-glycoproteins	1345:1371	3437 intact N-glycoproteins	1345:1371	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	2	26	from	countries	307:315	arg1	common					287:292	common	287:292	common	287:292	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	6	27	theme	stable	971:976	arg1	labeling					994:1001	stable diethyl isotope labeling	971:1001	stable diethyl isotope labeling	971:1001	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	0	28	theme	N-glycoprotein	107:120	arg1	markers					122:128	potential N-glycoprotein markers	97:128	potential N-glycoprotein markers	97:128	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	8	29	theme	change	1421:1426	arg1	criteria					1399:1406	the criteria	1395:1406	the criteria of ≥1.5-fold change and p value<0.05	1395:1443	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	3	30	theme	pancreatic	406:415	arg1	cancer					417:422	pancreatic cancer	406:422	pancreatic cancer	406:422	Currently, pancreatic cancer generally has a poor prognosis, partly due to the lack of symptoms in the early stages of pancreatic cancer.					
33835347	7	31	theme	FDR	1167:1169	arg1	%					1173:1173	spectrum-level FDR ≤1%	1152:1173	spectrum-level FDR ≤1%	1152:1173	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	6	32	theme	quantitative	1058:1069	arg1	N-glycoproteomics					1078:1094	site- and structure-specific quantitative tissue N-glycoproteomics	1029:1094	site- and structure-specific quantitative tissue N-glycoproteomics	1029:1094	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	8	33	theme	p	1432:1432	arg1	criteria					1399:1406	the criteria	1395:1406	the criteria of ≥1.5-fold change and p value<0.05	1395:1443	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	2	34	theme	death	358:362	arg1	causes					348:353	the main causes	339:353	the main causes of death in some countries and regions	339:392	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	7	35	theme	intact	1183:1188	arg1	N-Glycopeptides					1190:1204	20,038 intact N-Glycopeptides	1176:1204	20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins	1176:1371	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	7	36	dep	compositions	1275:1286	arg1	structures					1311:1320	1026 N-glycan putative structures	1288:1320	228 N-glycan monosaccharide compositions 1026 N-glycan putative structures	1247:1320	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	0	37	theme	site-	13:17	arg1	N-glycoproteomics					62:78	site- and structure-specific quantitative tissue N-glycoproteomics	13:78	site- and structure-specific quantitative tissue N-glycoproteomics	13:78	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	0	38	theme	structure-specific	23:40	arg1	N-glycoproteomics					62:78	site- and structure-specific quantitative tissue N-glycoproteomics	13:78	site- and structure-specific quantitative tissue N-glycoproteomics	13:78	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	5	39	theme	cells	749:753	arg1	analysis					722:729	quantitative analysis	709:729	quantitative analysis of cancer-related cells, plasma or tissues	709:772	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	2	40	from	death	358:362	arg1	regions					386:392	regions	386:392	regions	386:392	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	2	40	from	death	358:362	arg1	countries					372:380	some countries	367:380	some countries	367:380	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	7	41	theme	intact	1350:1355	arg1	N-glycoproteins					1357:1371	3437 intact N-glycoproteins	1345:1371	3437 intact N-glycoproteins	1345:1371	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	5	42	theme	quantitative	709:720	arg1	analysis					722:729	quantitative analysis	709:729	quantitative analysis of cancer-related cells, plasma or tissues	709:772	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	8	43	theme	intact	1474:1479	arg1	DEGPs					1498:1502	DEGPs	1498:1502	DEGPs	1498:1502	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	8	43	theme	intact	1474:1479	arg1	N-glycopeptides					1481:1495	52 differentially expressed intact N-glycopeptides	1446:1495	52 differentially expressed intact N-glycopeptides (DEGPs)	1446:1503	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	5	44	theme	plasma	756:761	arg1	analysis					722:729	quantitative analysis	709:729	quantitative analysis of cancer-related cells, plasma or tissues	709:772	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	7	45	gly	4460 N-glycosites	1323:1339	arg2	4460 N-glycosites					1323:1339	4460 N-glycosites	1323:1339	4460 N-glycosites	1323:1339	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	6	46	theme	markers	1138:1144	arg1	discovery					1100:1108	discovery	1100:1108	discovery of potential N-glycoprotein markers	1100:1144	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	1	47	theme	tract	229:233	arg1	difficult					243:251	difficult	243:251	difficult	243:251	Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat.					
33835347	1	47	theme	tract	229:233	arg1	tumor					206:210	a highly malignant tumor	187:210	a highly malignant tumor of the digestive tract that is difficult to diagnose and treat	187:273	Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat.					
33835347	1	47	theme	tract	229:233	arg1	cancer					177:182	Pancreatic cancer	166:182	Pancreatic cancer	166:182	Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat.					
33835347	6	48	theme	potential	1113:1121	arg1	markers					1138:1144	potential N-glycoprotein markers	1113:1144	potential N-glycoprotein markers	1113:1144	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	2	49	theme	causes	348:353	arg1	one					332:334	one	332:334	one	332:334	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	2	49	theme	causes	348:353	arg1	causes					348:353	the main causes	339:353	the main causes of death in some countries and regions	339:392	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	6	50	theme	glycopeptide	882:893	arg1	engine					911:916	complete N-linked glycopeptide database search engine	864:916	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	0	51	dep	Benchmark	0:8	arg1	study					138:142	a case study	131:142	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.	0:164	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	7	52	theme	putative	1302:1309	arg1	structures					1311:1320	1026 N-glycan putative structures	1288:1320	228 N-glycan monosaccharide compositions 1026 N-glycan putative structures	1247:1320	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	7	53	theme	peptide	1228:1234	arg1	backbones					1236:1244	4518 peptide backbones	1223:1244	4518 peptide backbones	1223:1244	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	8	54	gly	N-glycopeptides	1481:1495	arg2	DEGPs					1498:1502	DEGPs	1498:1502	DEGPs	1498:1502	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	8	54	gly	N-glycopeptides	1481:1495	arg2	N-glycopeptides					1481:1495	52 differentially expressed intact N-glycopeptides	1446:1495	52 differentially expressed intact N-glycopeptides (DEGPs)	1446:1503	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	6	55	theme	complete	864:871	arg1	engine					911:916	complete N-linked glycopeptide database search engine	864:916	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	6	56	theme	N-glycoproteomics	1078:1094	arg1	benchmark					1016:1024	a benchmark	1014:1024	a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers	1014:1144	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	5	57	theme	in-depth	608:615	arg1	research					617:624	the continuous in-depth research	593:624	the continuous in-depth research of glycoproteomics in precision medical diagnosis	593:674	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	3	58	theme	symptoms	482:489	arg1	lack					474:477	the lack	470:477	the lack of symptoms in the early stages of pancreatic cancer	470:530	Currently, pancreatic cancer generally has a poor prognosis, partly due to the lack of symptoms in the early stages of pancreatic cancer.					
33835347	0	59	theme	tissue	55:60	arg1	N-glycoproteomics					62:78	site- and structure-specific quantitative tissue N-glycoproteomics	13:78	site- and structure-specific quantitative tissue N-glycoproteomics	13:78	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	3	60	theme	pancreatic	514:523	arg1	cancer					525:530	pancreatic cancer	514:530	pancreatic cancer	514:530	Currently, pancreatic cancer generally has a poor prognosis, partly due to the lack of symptoms in the early stages of pancreatic cancer.					
33835347	6	61	theme	liquid-mass	942:952	arg1	spectrometry					954:965	liquid-mass spectrometry	942:965	liquid-mass spectrometry	942:965	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	2	62	from	causes	348:353	arg1	regions					386:392	regions	386:392	regions	386:392	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	2	62	from	causes	348:353	arg1	countries					372:380	some countries	367:380	some countries	367:380	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	5	63	theme	medical	658:664	arg1	diagnosis					666:674	precision medical diagnosis	648:674	precision medical diagnosis	648:674	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	5	64	theme	specific	782:789	arg1	biomarkers					791:800	specific biomarkers	782:800	specific biomarkers for targeted therapy	782:821	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	3	65	theme	cancer	525:530	arg1	stages					504:509	the early stages	494:509	the early stages of pancreatic cancer	494:530	Currently, pancreatic cancer generally has a poor prognosis, partly due to the lack of symptoms in the early stages of pancreatic cancer.					
33835347	3	66	contain	has	434:436	arg2	prognosis					445:453	a poor prognosis	438:453	a poor prognosis	438:453	Currently, pancreatic cancer generally has a poor prognosis, partly due to the lack of symptoms in the early stages of pancreatic cancer.					
33835347	3	66	contain	has	434:436	arg1	cancer					417:422	pancreatic cancer	406:422	pancreatic cancer	406:422	Currently, pancreatic cancer generally has a poor prognosis, partly due to the lack of symptoms in the early stages of pancreatic cancer.					
33835347	4	67	theme	advanced	572:579	arg1	stage					581:585	advanced stage	572:585	advanced stage	572:585	Therefore, most cases are diagnosed at advanced stage.					
33835347	0	68	theme	pancreatic	147:156	arg1	cancer					158:163	pancreatic cancer	147:163	pancreatic cancer	147:163	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	5	69	from	reports	698:704	arg1	analysis					722:729	quantitative analysis	709:729	quantitative analysis of cancer-related cells, plasma or tissues	709:772	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	8	70	theme	pancreatic	1519:1528	arg1	tussues					1537:1543	pancreatic cancer tussues	1519:1543	pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively	1519:1622	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	5	71	with	reports	698:704	arg1	research					617:624	the continuous in-depth research	593:624	the continuous in-depth research of glycoproteomics in precision medical diagnosis	593:674	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	6	72	theme	search	904:909	arg1	engine					911:916	complete N-linked glycopeptide database search engine	864:916	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	0	73	theme	quantitative	42:53	arg1	N-glycoproteomics					62:78	site- and structure-specific quantitative tissue N-glycoproteomics	13:78	site- and structure-specific quantitative tissue N-glycoproteomics	13:78	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	8	74	located	found	1510:1514	arg1	tussues					1537:1543	pancreatic cancer tussues	1519:1543	pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively	1519:1622	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	8	74	located	found	1510:1514	arg2	DEGPs					1498:1502	DEGPs	1498:1502	DEGPs	1498:1502	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	8	74	located	found	1510:1514	arg2	N-glycopeptides					1481:1495	52 differentially expressed intact N-glycopeptides	1446:1495	52 differentially expressed intact N-glycopeptides (DEGPs)	1446:1503	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	7	75	theme	4518	1223:1226	arg1	backbones					1236:1244	4518 peptide backbones	1223:1244	4518 peptide backbones	1223:1244	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	0	76	theme	potential	97:105	arg1	markers					122:128	potential N-glycoprotein markers	97:128	potential N-glycoprotein markers	97:128	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	1	77	theme	Pancreatic	166:175	arg1	difficult					243:251	difficult	243:251	difficult	243:251	Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat.					
33835347	1	77	theme	Pancreatic	166:175	arg1	tumor					206:210	a highly malignant tumor	187:210	a highly malignant tumor of the digestive tract that is difficult to diagnose and treat	187:273	Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat.					
33835347	1	77	theme	Pancreatic	166:175	arg1	cancer					177:182	Pancreatic cancer	166:182	Pancreatic cancer	166:182	Pancreatic cancer is a highly malignant tumor of the digestive tract that is difficult to diagnose and treat.					
33835347	2	78	from	common	287:292	arg1	countries					307:315	developed countries	297:315	developed countries	297:315	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	6	79	theme	diethyl	978:984	arg1	labeling					994:1001	stable diethyl isotope labeling	971:1001	stable diethyl isotope labeling	971:1001	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	0	80	theme	markers	122:128	arg1	discovery					84:92	discovery	84:92	discovery of potential N-glycoprotein markers	84:128	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	6	81	theme	tissue	1071:1076	arg1	N-glycoproteomics					1078:1094	site- and structure-specific quantitative tissue N-glycoproteomics	1029:1094	site- and structure-specific quantitative tissue N-glycoproteomics	1029:1094	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	8	82	theme	≥1.5-fold	1411:1419	arg1	change					1421:1426	≥1.5-fold change	1411:1426	≥1.5-fold change	1411:1426	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	0	83	gly	N-glycoprotein	107:120	arg1	N-glycoprotein					107:120	potential N-glycoprotein markers	97:128	potential N-glycoprotein markers	97:128	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	6	84	theme	structure-specific	1039:1056	arg1	N-glycoproteomics					1078:1094	site- and structure-specific quantitative tissue N-glycoproteomics	1029:1094	site- and structure-specific quantitative tissue N-glycoproteomics	1029:1094	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	6	85	gly	N-glycoprotein	1123:1136	arg1	N-glycoprotein					1123:1136	potential N-glycoprotein markers	1113:1144	potential N-glycoprotein markers	1113:1144	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	6	86	link	N-linked	873:880	arg1	engine					911:916	complete N-linked glycopeptide database search engine	864:916	the developed complete N-linked glycopeptide database search engine GPSeeker	850:925	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	6	87	theme	site-	1029:1033	arg1	N-glycoproteomics					1078:1094	site- and structure-specific quantitative tissue N-glycoproteomics	1029:1094	site- and structure-specific quantitative tissue N-glycoproteomics	1029:1094	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	3	88	theme	poor	440:443	arg1	prognosis					445:453	a poor prognosis	438:453	a poor prognosis	438:453	Currently, pancreatic cancer generally has a poor prognosis, partly due to the lack of symptoms in the early stages of pancreatic cancer.					
33835347	5	89	theme	cancer-related	734:747	arg1	cells					749:753	cancer-related cells	734:753	cancer-related cells	734:753	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	0	90	theme	N-glycoproteomics	62:78	arg1	Benchmark					0:8	Benchmark	0:8	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.	0:164	Benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers: a case study of pancreatic cancer.					
33835347	5	91	from	research	617:624	arg1	diagnosis					666:674	precision medical diagnosis	648:674	precision medical diagnosis	648:674	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	8	92	theme	expressed	1464:1472	arg1	DEGPs					1498:1502	DEGPs	1498:1502	DEGPs	1498:1502	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	8	92	theme	expressed	1464:1472	arg1	N-glycopeptides					1481:1495	52 differentially expressed intact N-glycopeptides	1446:1495	52 differentially expressed intact N-glycopeptides (DEGPs)	1446:1503	With the criteria of ≥1.5-fold change and p value<0.05, 52 differentially expressed intact N-glycopeptides (DEGPs) were found in pancreatic cancer tussues relative to control, where 38 up-regulated and 14 down-regulated, respectively.					
33835347	3	93	theme	due	463:465	arg1	prognosis					445:453	a poor prognosis	438:453	a poor prognosis	438:453	Currently, pancreatic cancer generally has a poor prognosis, partly due to the lack of symptoms in the early stages of pancreatic cancer.					
33835347	6	94	theme	N-glycoprotein	1123:1136	arg1	markers					1138:1144	potential N-glycoprotein markers	1113:1144	potential N-glycoprotein markers	1113:1144	This research is based on the developed complete N-linked glycopeptide database search engine GPSeeker, combined with liquid-mass spectrometry and stable diethyl isotope labeling, providing a benchmark of site- and structure-specific quantitative tissue N-glycoproteomics for discovery of potential N-glycoprotein markers.					
33835347	5	95	theme	targeted	806:813	arg1	therapy					815:821	targeted therapy	806:821	targeted therapy	806:821	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33835347	2	96	from	countries	372:380	arg1	causes					348:353	the main causes	339:353	the main causes of death in some countries and regions	339:392	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	7	97	theme	1026 N-glycan	1288:1300	arg1	structures					1311:1320	1026 N-glycan putative structures	1288:1320	228 N-glycan monosaccharide compositions 1026 N-glycan putative structures	1247:1320	With spectrum-level FDR ≤1%, 20,038 intact N-Glycopeptides corresponding to 4518 peptide backbones, 228 N-glycan monosaccharide compositions 1026 N-glycan putative structures, 4460 N-glycosites and 3437 intact N-glycoproteins were identified.					
33835347	2	98	theme	main	343:346	arg1	causes					348:353	the main causes	339:353	the main causes of death in some countries and regions	339:392	It is more common in developed countries and has become one of the main causes of death in some countries and regions.					
33835347	5	99	from	glycoproteomics	629:643	arg1	diagnosis					666:674	precision medical diagnosis	648:674	precision medical diagnosis	648:674	With the continuous in-depth research of glycoproteomics in precision medical diagnosis, there have been some reports on quantitative analysis of cancer-related cells, plasma or tissues to find specific biomarkers for targeted therapy.					
33063473	3	0	located	observed	456:463	arg2	glycosylation					433:445	dysregulated glycosylation	420:445	dysregulated glycosylation	420:445	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	3	0	located	observed	456:463	arg1	microenvironment					478:493	the tumor microenvironment	468:493	the tumor microenvironment	468:493	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	5	1	theme	light	884:888	arg1	chain					890:894	the light chain	880:894	the light chain	880:894	The structure of the camrelizumab/PD-1 complex reveals that camrelizumab mainly utilizes its heavy chain to bind to PD-1, while the light chain sterically inhibits the binding of PD-L1 to PD-1.					
33063473	7	2	theme	MAbs	1255:1258	arg1	activity					1229:1236	the activity	1225:1236	the activity of PD-1-specific MAbs	1225:1258	These results increase our understanding of how glycosylation affects the activity of PD-1-specific MAbs during immune checkpoint therapy.					
33063473	7	3	gly	glycosylation	1203:1215	arg1	MAbs					1255:1258	PD-1-specific MAbs	1241:1258	PD-1-specific MAbs	1241:1258	These results increase our understanding of how glycosylation affects the activity of PD-1-specific MAbs during immune checkpoint therapy.					
33063473	1	4	gly	glycosylated	75:86	arg1	PD-1					58:61	PD-1	58:61	PD-1	58:61	PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells.					
33063473	1	4	gly	glycosylated	75:86	arg1	receptor					99:106	a highly glycosylated inhibitory receptor	66:106	a highly glycosylated inhibitory receptor expressed mainly on T cells	66:134	PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells.					
33063473	2	5	theme	PD-1	150:153	arg1	Targeting					137:145	Targeting	137:145	Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1	137:234	Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors.					
33063473	7	6	theme	PD-1-specific	1241:1253	arg1	MAbs					1255:1258	PD-1-specific MAbs	1241:1258	PD-1-specific MAbs	1241:1258	These results increase our understanding of how glycosylation affects the activity of PD-1-specific MAbs during immune checkpoint therapy.					
33063473	4	7	theme	approved	617:624	arg1	MAb					640:642	a recently approved PD-1-specific MAb	606:642	a recently approved PD-1-specific MAb	606:642	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	4	7	theme	approved	617:624	arg1	camrelizumab					592:603	camrelizumab	592:603	camrelizumab	592:603	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	3	8	theme	N-glycosylation	320:334	arg1	sites					336:340	N-glycosylation sites	320:340	N-glycosylation sites of PD-1	320:348	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	3	8	theme	N-glycosylation	320:334	arg1	PD-1					345:348	PD-1	345:348	PD-1	345:348	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	5	9	theme	camrelizumab/PD-1	773:789	arg1	complex					791:797	the camrelizumab/PD-1 complex	769:797	the camrelizumab/PD-1 complex	769:797	The structure of the camrelizumab/PD-1 complex reveals that camrelizumab mainly utilizes its heavy chain to bind to PD-1, while the light chain sterically inhibits the binding of PD-L1 to PD-1.					
33063473	4	10	gly	glycosylated	733:744	arg1	PD-1					746:749	glycosylated PD-1	733:749	glycosylated PD-1	733:749	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	6	11	theme	asparagine	963:972	arg1	Glycosylation					946:958	Glycosylation	946:958	Glycosylation of asparagine 58 (N58)	946:981	Glycosylation of asparagine 58 (N58) promotes the interaction with camrelizumab, while the efficiency of camrelizumab to inhibit the binding of PD-L1 is substantially reduced for glycosylation-deficient PD-1.					
33063473	1	12	theme	glycosylated	75:86	arg1	PD-1					58:61	PD-1	58:61	PD-1	58:61	PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells.					
33063473	1	12	theme	glycosylated	75:86	arg1	receptor					99:106	a highly glycosylated inhibitory receptor	66:106	a highly glycosylated inhibitory receptor expressed mainly on T cells	66:134	PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells.					
33063473	3	13	theme	antibody	398:405	arg1	binding					407:413	antibody binding	398:413	antibody binding	398:413	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	3	14	theme	human	363:367	arg1	population					369:378	the human population	359:378	the human population that might affect antibody binding	359:413	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	2	15	with	interaction	202:212	arg1	PD-L1					230:234	its ligand PD-L1	219:234	its ligand PD-L1	219:234	Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors.					
33063473	2	16	theme	tumors	286:291	arg1	treatment					264:272	the treatment	260:272	the treatment of multiple tumors	260:291	Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors.					
33063473	3	17	from	sites	336:340	arg1	polymorphisms					303:315	polymorphisms	303:315	polymorphisms at N-glycosylation sites of PD-1	303:348	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	6	18	theme	camrelizumab	1051:1062	arg1	efficiency					1037:1046	the efficiency	1033:1046	the efficiency of camrelizumab to inhibit the binding of PD-L1	1033:1094	Glycosylation of asparagine 58 (N58) promotes the interaction with camrelizumab, while the efficiency of camrelizumab to inhibit the binding of PD-L1 is substantially reduced for glycosylation-deficient PD-1.					
33063473	6	19	gly	Glycosylation	946:958	arg1	N58					978:980	N58	978:980	N58	978:980	Glycosylation of asparagine 58 (N58) promotes the interaction with camrelizumab, while the efficiency of camrelizumab to inhibit the binding of PD-L1 is substantially reduced for glycosylation-deficient PD-1.					
33063473	6	19	gly	Glycosylation	946:958	arg1	asparagine					963:972	asparagine 58	963:975	asparagine 58 (N58)	963:981	Glycosylation of asparagine 58 (N58) promotes the interaction with camrelizumab, while the efficiency of camrelizumab to inhibit the binding of PD-L1 is substantially reduced for glycosylation-deficient PD-1.					
33063473	2	20	theme	multiple	277:284	arg1	tumors					286:291	multiple tumors	277:291	multiple tumors	277:291	Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors.					
33063473	1	21	theme	inhibitory	88:97	arg1	PD-1					58:61	PD-1	58:61	PD-1	58:61	PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells.					
33063473	1	21	theme	inhibitory	88:97	arg1	receptor					99:106	a highly glycosylated inhibitory receptor	66:106	a highly glycosylated inhibitory receptor expressed mainly on T cells	66:134	PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells.					
33063473	5	22	theme	PD-L1	931:935	arg1	binding					920:926	the binding	916:926	the binding of PD-L1 to PD-1	916:943	The structure of the camrelizumab/PD-1 complex reveals that camrelizumab mainly utilizes its heavy chain to bind to PD-1, while the light chain sterically inhibits the binding of PD-L1 to PD-1.					
33063473	4	23	theme	glycosylated	733:744	arg1	PD-1					746:749	glycosylated PD-1	733:749	glycosylated PD-1	733:749	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	5	24	theme	heavy	845:849	arg1	chain					851:855	its heavy chain	841:855	its heavy chain	841:855	The structure of the camrelizumab/PD-1 complex reveals that camrelizumab mainly utilizes its heavy chain to bind to PD-1, while the light chain sterically inhibits the binding of PD-L1 to PD-1.					
33063473	3	25	theme	dysregulated	420:431	arg1	glycosylation					433:445	dysregulated glycosylation	420:445	dysregulated glycosylation	420:445	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	0	26	gly	N-glycosylation	0:14	arg1	PD-1					19:22	PD-1	19:22	PD-1	19:22	N-glycosylation of PD-1 promotes binding of camrelizumab.					
33063473	4	27	from	E. coli	684:690	arg1	proteins					670:677	non-glycosylated PD-1 proteins	648:677	non-glycosylated PD-1 proteins from E. coli	648:690	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	2	28	with	PD-1	150:153	arg1	antibodies					171:180	monoclonal antibodies	160:180	monoclonal antibodies (MAbs)	160:187	Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors.					
33063473	2	28	with	PD-1	150:153	arg1	MAbs					183:186	MAbs	183:186	MAbs	183:186	Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors.					
33063473	0	29	theme	PD-1	19:22	arg1	N-glycosylation					0:14	N-glycosylation	0:14	N-glycosylation of PD-1	0:22	N-glycosylation of PD-1 promotes binding of camrelizumab.					
33063473	3	30	gly	N-glycosylation	320:334	arg2	PD-1					345:348	PD-1	345:348	PD-1	345:348	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	3	30	gly	N-glycosylation	320:334	arg2	sites					336:340	N-glycosylation sites	320:340	N-glycosylation sites of PD-1	320:348	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	3	30	gly	N-glycosylation	320:334	arg1	PD-1					345:348	PD-1	345:348	PD-1	345:348	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	2	31	theme	monoclonal	160:169	arg1	antibodies					171:180	monoclonal antibodies	160:180	monoclonal antibodies (MAbs)	160:187	Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors.					
33063473	2	31	theme	monoclonal	160:169	arg1	MAbs					183:186	MAbs	183:186	MAbs	183:186	Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors.					
33063473	5	32	theme	complex	791:797	arg1	structure					756:764	The structure	752:764	The structure of the camrelizumab/PD-1 complex	752:797	The structure of the camrelizumab/PD-1 complex reveals that camrelizumab mainly utilizes its heavy chain to bind to PD-1, while the light chain sterically inhibits the binding of PD-L1 to PD-1.					
33063473	4	33	gly	non-glycosylated	648:663	arg1	proteins					670:677	non-glycosylated PD-1 proteins	648:677	non-glycosylated PD-1 proteins from E. coli	648:690	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	3	34	gly	glycosylation	433:445	arg1	microenvironment					478:493	the tumor microenvironment	468:493	the tumor microenvironment	468:493	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	0	35	theme	camrelizumab	44:55	arg1	binding					33:39	binding	33:39	binding of camrelizumab	33:55	N-glycosylation of PD-1 promotes binding of camrelizumab.					
33063473	4	36	theme	N-glycan	524:531	arg1	composition					533:543	varied N-glycan composition	517:543	varied N-glycan composition in PD-1	517:551	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	6	37	with	interaction	996:1006	arg1	camrelizumab					1013:1024	camrelizumab	1013:1024	camrelizumab	1013:1024	Glycosylation of asparagine 58 (N58) promotes the interaction with camrelizumab, while the efficiency of camrelizumab to inhibit the binding of PD-L1 is substantially reduced for glycosylation-deficient PD-1.					
33063473	3	38	theme	tumor	472:476	arg1	microenvironment					478:493	the tumor microenvironment	468:493	the tumor microenvironment	468:493	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	4	39	theme	varied	517:522	arg1	composition					533:543	varied N-glycan composition	517:543	varied N-glycan composition in PD-1	517:551	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	7	40	theme	immune	1267:1272	arg1	therapy					1285:1291	immune checkpoint therapy	1267:1291	immune checkpoint therapy	1267:1291	These results increase our understanding of how glycosylation affects the activity of PD-1-specific MAbs during immune checkpoint therapy.					
33063473	1	41	theme	T	128:128	arg1	cells					130:134	T cells	128:134	T cells	128:134	PD-1 is a highly glycosylated inhibitory receptor expressed mainly on T cells.					
33063473	6	42	theme	glycosylation-deficient	1125:1147	arg1	PD-1					1149:1152	glycosylation-deficient PD-1	1125:1152	glycosylation-deficient PD-1	1125:1152	Glycosylation of asparagine 58 (N58) promotes the interaction with camrelizumab, while the efficiency of camrelizumab to inhibit the binding of PD-L1 is substantially reduced for glycosylation-deficient PD-1.					
33063473	4	43	from	composition	533:543	arg1	PD-1					548:551	PD-1	548:551	PD-1	548:551	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	6	44	theme	PD-L1	1090:1094	arg1	binding					1079:1085	the binding	1075:1085	the binding of PD-L1	1075:1094	Glycosylation of asparagine 58 (N58) promotes the interaction with camrelizumab, while the efficiency of camrelizumab to inhibit the binding of PD-L1 is substantially reduced for glycosylation-deficient PD-1.					
33063473	4	45	theme	binding	572:578	arg1	affinity					580:587	the binding affinity	568:587	the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli	568:690	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	4	46	theme	PD-1	665:668	arg1	proteins					670:677	non-glycosylated PD-1 proteins	648:677	non-glycosylated PD-1 proteins from E. coli	648:690	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	4	47	theme	PD-1-specific	626:638	arg1	MAb					640:642	a recently approved PD-1-specific MAb	606:642	a recently approved PD-1-specific MAb	606:642	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	4	47	theme	PD-1-specific	626:638	arg1	camrelizumab					592:603	camrelizumab	592:603	camrelizumab	592:603	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	4	48	theme	camrelizumab	592:603	arg1	affinity					580:587	the binding affinity	568:587	the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli	568:690	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	2	49	theme	ligand	223:228	arg1	PD-L1					230:234	its ligand PD-L1	219:234	its ligand PD-L1	219:234	Targeting of PD-1 with monoclonal antibodies (MAbs) to block the interaction with its ligand PD-L1 has been successful for the treatment of multiple tumors.					
33063473	7	50	theme	checkpoint	1274:1283	arg1	therapy					1285:1291	immune checkpoint therapy	1267:1291	immune checkpoint therapy	1267:1291	These results increase our understanding of how glycosylation affects the activity of PD-1-specific MAbs during immune checkpoint therapy.					
33063473	4	51	theme	non-glycosylated	648:663	arg1	proteins					670:677	non-glycosylated PD-1 proteins	648:677	non-glycosylated PD-1 proteins from E. coli	648:690	Here, we demonstrate varied N-glycan composition in PD-1, and show that the binding affinity of camrelizumab, a recently approved PD-1-specific MAb, to non-glycosylated PD-1 proteins from E. coli is substantially decreased compared with glycosylated PD-1.					
33063473	3	52	theme	PD-1	345:348	arg1	sites					336:340	N-glycosylation sites	320:340	N-glycosylation sites of PD-1	320:348	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
33063473	3	52	theme	PD-1	345:348	arg1	PD-1					345:348	PD-1	345:348	PD-1	345:348	However, polymorphisms at N-glycosylation sites of PD-1 exist in the human population that might affect antibody binding, and dysregulated glycosylation has been observed in the tumor microenvironment.					
32407692	3	0	theme	stress	366:371	arg1	tolerance					373:381	abiotic stress tolerance	358:381	abiotic stress tolerance	358:381	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	1	1	theme	Glycosylinositol	62:77	arg1	GIPCs					100:104	GIPCs	100:104	GIPCs	100:104	Glycosylinositol phosphorylceramides (GIPCs) constitute an important class of plasma-membrane lipids in plants.					
32407692	1	1	theme	Glycosylinositol	62:77	arg1	phosphorylceramides					79:97	Glycosylinositol phosphorylceramides	62:97	Glycosylinositol phosphorylceramides (GIPCs)	62:105	Glycosylinositol phosphorylceramides (GIPCs) constitute an important class of plasma-membrane lipids in plants.					
32407692	3	2	theme	abiotic	358:364	arg1	tolerance					373:381	abiotic stress tolerance	358:381	abiotic stress tolerance	358:381	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	0	3	theme	Sphingolipid	34:45	arg1	Glycosylation					47:59	Complex Sphingolipid Glycosylation	26:59	Complex Sphingolipid Glycosylation	26:59	Synthesis and Function of Complex Sphingolipid Glycosylation.					
32407692	3	4	theme	Recent	253:258	arg1	studies					260:266	Recent studies	253:266	Recent studies	253:266	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	1	5	from	class	131:135	arg1	plants					166:171	plants	166:171	plants	166:171	Glycosylinositol phosphorylceramides (GIPCs) constitute an important class of plasma-membrane lipids in plants.					
32407692	3	6	with	interactions	388:399	arg1	microorganisms					431:444	pathogenic and symbiotic microorganisms	406:444	pathogenic and symbiotic microorganisms	406:444	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	1	7	theme	important	121:129	arg1	class					131:135	an important class	118:135	an important class of plasma-membrane lipids in plants	118:171	Glycosylinositol phosphorylceramides (GIPCs) constitute an important class of plasma-membrane lipids in plants.					
32407692	3	8	theme	symbiotic	421:429	arg1	microorganisms					431:444	pathogenic and symbiotic microorganisms	406:444	pathogenic and symbiotic microorganisms	406:444	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	0	9	theme	Complex	26:32	arg1	Glycosylation					47:59	Complex Sphingolipid Glycosylation	26:59	Complex Sphingolipid Glycosylation	26:59	Synthesis and Function of Complex Sphingolipid Glycosylation.					
32407692	2	10	theme	glycan	186:191	arg1	headgroups					193:202	The complex glycan headgroups	174:202	The complex glycan headgroups of GIPCs	174:211	The complex glycan headgroups of GIPCs vary between plant species and tissues.					
32407692	3	11	theme	pathogenic	406:415	arg1	microorganisms					431:444	pathogenic and symbiotic microorganisms	406:444	pathogenic and symbiotic microorganisms	406:444	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	3	12	theme	headgroup	312:320	arg1	structure					288:296	the structure	284:296	the structure of the glycan headgroup	284:320	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	3	12	theme	headgroup	312:320	arg1	important					325:333	important	325:333	important	325:333	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	2	13	theme	complex	178:184	arg1	headgroups					193:202	The complex glycan headgroups	174:202	The complex glycan headgroups of GIPCs	174:211	The complex glycan headgroups of GIPCs vary between plant species and tissues.					
32407692	0	14	theme	Glycosylation	47:59	arg1	Function					14:21	Function	14:21	Function	14:21	Synthesis and Function of Complex Sphingolipid Glycosylation.					
32407692	0	14	theme	Glycosylation	47:59	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and Function of Complex Sphingolipid Glycosylation.					
32407692	2	15	theme	plant	226:230	arg1	species					232:238	plant species	226:238	plant species	226:238	The complex glycan headgroups of GIPCs vary between plant species and tissues.					
32407692	3	16	with	tolerance	373:381	arg1	microorganisms					431:444	pathogenic and symbiotic microorganisms	406:444	pathogenic and symbiotic microorganisms	406:444	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	1	17	theme	plasma-membrane	140:154	arg1	lipids					156:161	plasma-membrane lipids	140:161	plasma-membrane lipids	140:161	Glycosylinositol phosphorylceramides (GIPCs) constitute an important class of plasma-membrane lipids in plants.					
32407692	3	18	theme	glycan	305:310	arg1	headgroup					312:320	the glycan headgroup	301:320	the glycan headgroup	301:320	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	1	19	theme	lipids	156:161	arg1	class					131:135	an important class	118:135	an important class of plasma-membrane lipids in plants	118:171	Glycosylinositol phosphorylceramides (GIPCs) constitute an important class of plasma-membrane lipids in plants.					
32407692	3	20	theme	plant	339:343	arg1	development					345:355	plant development	339:355	plant development	339:355	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32407692	2	21	theme	GIPCs	207:211	arg1	headgroups					193:202	The complex glycan headgroups	174:202	The complex glycan headgroups of GIPCs	174:211	The complex glycan headgroups of GIPCs vary between plant species and tissues.					
32407692	3	22	with	development	345:355	arg1	microorganisms					431:444	pathogenic and symbiotic microorganisms	406:444	pathogenic and symbiotic microorganisms	406:444	Recent studies have shown that the structure of the glycan headgroup is important for plant development, abiotic stress tolerance, and interactions with pathogenic and symbiotic microorganisms.					
32413590	2	0	theme	other	690:694	arg1	methods					716:722	any other previously reported methods	686:722	any other previously reported methods	686:722	This new method is facile and applicable to non-labeled carbohydrates, and it is extremely sensitive, more so than any other previously reported methods.					
32413590	1	1	theme	d-glucose-containing	240:259	arg1	laminaribiose					317:329	laminaribiose	317:329	laminaribiose	317:329	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	1	theme	d-glucose-containing	240:259	arg1	isomaltose					353:362	isomaltose	353:362	isomaltose	353:362	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	1	theme	d-glucose-containing	240:259	arg1	kojibiose					285:293	kojibiose	285:293	kojibiose	285:293	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	1	theme	d-glucose-containing	240:259	arg1	gentiobiose					369:379	gentiobiose	369:379	gentiobiose	369:379	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	1	theme	d-glucose-containing	240:259	arg1	sophorose					296:304	sophorose	296:304	sophorose	296:304	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	1	theme	d-glucose-containing	240:259	arg1	maltose					332:338	maltose	332:338	maltose	332:338	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	1	theme	d-glucose-containing	240:259	arg1	cellobiose					341:350	cellobiose	341:350	cellobiose	341:350	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	1	theme	d-glucose-containing	240:259	arg1	disaccharides					270:282	all d-glucose-containing reducing disaccharides	236:282	all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose)	236:380	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	1	theme	d-glucose-containing	240:259	arg1	nigerose					307:314	nigerose	307:314	nigerose	307:314	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	6	2	theme	1-4-linked	1183:1192	arg1	structures					1194:1203	The 1-4-linked structures	1179:1203	The 1-4-linked structures	1179:1203	The 1-4-linked structures, involved in the formation of amylose and cellulose, respectively, were found to be advantageous due to their relatively low susceptibility to glycation.					
32413590	6	2	theme	1-4-linked	1183:1192	arg1	advantageous					1289:1300	advantageous	1289:1300	advantageous	1289:1300	The 1-4-linked structures, involved in the formation of amylose and cellulose, respectively, were found to be advantageous due to their relatively low susceptibility to glycation.					
32413590	1	3	theme	reducing	261:268	arg1	laminaribiose					317:329	laminaribiose	317:329	laminaribiose	317:329	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	3	theme	reducing	261:268	arg1	isomaltose					353:362	isomaltose	353:362	isomaltose	353:362	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	3	theme	reducing	261:268	arg1	kojibiose					285:293	kojibiose	285:293	kojibiose	285:293	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	3	theme	reducing	261:268	arg1	gentiobiose					369:379	gentiobiose	369:379	gentiobiose	369:379	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	3	theme	reducing	261:268	arg1	sophorose					296:304	sophorose	296:304	sophorose	296:304	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	3	theme	reducing	261:268	arg1	maltose					332:338	maltose	332:338	maltose	332:338	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	3	theme	reducing	261:268	arg1	cellobiose					341:350	cellobiose	341:350	cellobiose	341:350	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	3	theme	reducing	261:268	arg1	disaccharides					270:282	all d-glucose-containing reducing disaccharides	236:282	all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose)	236:380	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	3	theme	reducing	261:268	arg1	nigerose					307:314	nigerose	307:314	nigerose	307:314	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	0	4	theme	open-ring	185:193	arg1	form					195:198	the open-ring form	181:198	the open-ring form	181:198	Analysis of the susceptibility of reducing disaccharides composed of d-glucose to glycation using the Maillard reaction and a novel sensitive method that measures the percentage of the open-ring form.					
32413590	4	5	attach	linked	980:985	arg3	bonds					1007:1011	1-2, 1-3 and 1-4 bonds	990:1011	1-2, 1-3 and 1-4 bonds	990:1011	On the other hand, the proportion of acyclic form was generally very low when glucoses were linked by 1-2, 1-3 and 1-4 bonds.					
32413590	4	5	attach	linked	980:985	arg2	glucoses					966:973	glucoses	966:973	glucoses	966:973	On the other hand, the proportion of acyclic form was generally very low when glucoses were linked by 1-2, 1-3 and 1-4 bonds.					
32413590	3	6	theme	greatest	851:858	arg1	form					882:885	the acyclic form	870:885	the acyclic form	870:885	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	3	6	theme	greatest	851:858	arg1	amount					860:865	the greatest amount	847:865	the greatest amount of the acyclic form	847:885	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	1	7	theme	disaccharides	270:282	arg1	glycation					223:231	glycation	223:231	glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose)	223:380	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	2	8	theme	new	576:578	arg1	method					580:585	This new method	571:585	This new method	571:585	This new method is facile and applicable to non-labeled carbohydrates, and it is extremely sensitive, more so than any other previously reported methods.					
32413590	3	9	theme	form	882:885	arg1	form					882:885	the acyclic form	870:885	the acyclic form	870:885	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	3	9	theme	form	882:885	arg1	amount					860:865	the greatest amount	847:865	the greatest amount of the acyclic form	847:885	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	3	10	theme	oxime	806:810	arg1	reactivity					822:831	oxime formation reactivity	806:831	oxime formation reactivity	806:831	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	2	11	theme	non-labeled	615:625	arg1	carbohydrates					627:639	non-labeled carbohydrates	615:639	non-labeled carbohydrates	615:639	This new method is facile and applicable to non-labeled carbohydrates, and it is extremely sensitive, more so than any other previously reported methods.					
32413590	1	12	theme	acyclic	446:452	arg1	forms					454:458	their acyclic forms	440:458	their acyclic forms	440:458	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	0	13	theme	Maillard	102:109	arg1	reaction					111:118	the Maillard reaction	98:118	the Maillard reaction	98:118	Analysis of the susceptibility of reducing disaccharides composed of d-glucose to glycation using the Maillard reaction and a novel sensitive method that measures the percentage of the open-ring form.					
32413590	0	14	theme	form	195:198	arg1	percentage					167:176	the percentage	163:176	the percentage of the open-ring form	163:198	Analysis of the susceptibility of reducing disaccharides composed of d-glucose to glycation using the Maillard reaction and a novel sensitive method that measures the percentage of the open-ring form.					
32413590	1	15	theme	forms	454:458	arg1	percentages					425:435	the percentages	421:435	the percentages of their acyclic forms	421:458	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	5	16	theme	linkage	1039:1045	arg1	stability					1018:1026	The stability	1014:1026	The stability of the 1-3 linkage	1014:1045	The stability of the 1-3 linkage was drastically reduced when basicity was increased due to β-elimination and the production of a highly reactive dehydrated hexose.					
32413590	3	17	contain	had	843:845	arg1	they					838:841	they	838:841	they	838:841	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	3	17	contain	had	843:845	arg2	amount					860:865	the greatest amount	847:865	the greatest amount of the acyclic form	847:885	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	3	17	contain	had	843:845	arg2	form					882:885	the acyclic form	870:885	the acyclic form	870:885	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	3	18	theme	acyclic	874:880	arg1	form					882:885	the acyclic form	870:885	the acyclic form	870:885	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	1	19	theme	Maillard	399:406	arg1	browning					408:415	Maillard browning	399:415	Maillard browning	399:415	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	20	theme	first	559:563	arg1	time					565:568	the first time	555:568	the first time	555:568	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	0	21	theme	susceptibility	16:29	arg1	Analysis					0:7	Analysis	0:7	Analysis of the susceptibility of reducing disaccharides composed of d-glucose to glycation	0:90	Analysis of the susceptibility of reducing disaccharides composed of d-glucose to glycation using the Maillard reaction and a novel sensitive method that measures the percentage of the open-ring form.					
32413590	4	22	theme	other	895:899	arg1	hand					901:904	the other hand	891:904	the other hand	891:904	On the other hand, the proportion of acyclic form was generally very low when glucoses were linked by 1-2, 1-3 and 1-4 bonds.					
32413590	6	23	link	1-4-linked	1183:1192	arg1	structures					1194:1203	The 1-4-linked structures	1179:1203	The 1-4-linked structures	1179:1203	The 1-4-linked structures, involved in the formation of amylose and cellulose, respectively, were found to be advantageous due to their relatively low susceptibility to glycation.					
32413590	6	23	link	1-4-linked	1183:1192	arg1	advantageous					1289:1300	advantageous	1289:1300	advantageous	1289:1300	The 1-4-linked structures, involved in the formation of amylose and cellulose, respectively, were found to be advantageous due to their relatively low susceptibility to glycation.					
32413590	0	24	theme	reducing	34:41	arg1	disaccharides					43:55	reducing disaccharides	34:55	reducing disaccharides composed of d-glucose	34:77	Analysis of the susceptibility of reducing disaccharides composed of d-glucose to glycation using the Maillard reaction and a novel sensitive method that measures the percentage of the open-ring form.					
32413590	0	25	theme	sensitive	132:140	arg1	method					142:147	a novel sensitive method	124:147	a novel sensitive method that measures the percentage of the open-ring form	124:198	Analysis of the susceptibility of reducing disaccharides composed of d-glucose to glycation using the Maillard reaction and a novel sensitive method that measures the percentage of the open-ring form.					
32413590	0	26	theme	novel	126:130	arg1	method					142:147	a novel sensitive method	124:147	a novel sensitive method that measures the percentage of the open-ring form	124:198	Analysis of the susceptibility of reducing disaccharides composed of d-glucose to glycation using the Maillard reaction and a novel sensitive method that measures the percentage of the open-ring form.					
32413590	1	27	theme	novel	478:482	arg1	method					484:489	a novel method	476:489	a novel method to evaluate reactivity toward oxime formation were compared for the first time	476:568	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	6	28	theme	cellulose	1247:1255	arg1	formation					1222:1230	the formation	1218:1230	the formation of amylose and cellulose, respectively	1218:1269	The 1-4-linked structures, involved in the formation of amylose and cellulose, respectively, were found to be advantageous due to their relatively low susceptibility to glycation.					
32413590	0	29	theme	disaccharides	43:55	arg1	susceptibility					16:29	the susceptibility	12:29	the susceptibility of reducing disaccharides composed of d-glucose to glycation	12:90	Analysis of the susceptibility of reducing disaccharides composed of d-glucose to glycation using the Maillard reaction and a novel sensitive method that measures the percentage of the open-ring form.					
32413590	1	30	dep	disaccharides	270:282	arg1	laminaribiose					317:329	laminaribiose	317:329	laminaribiose	317:329	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	30	dep	disaccharides	270:282	arg1	isomaltose					353:362	isomaltose	353:362	isomaltose	353:362	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	30	dep	disaccharides	270:282	arg1	kojibiose					285:293	kojibiose	285:293	kojibiose	285:293	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	30	dep	disaccharides	270:282	arg1	gentiobiose					369:379	gentiobiose	369:379	gentiobiose	369:379	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	30	dep	disaccharides	270:282	arg1	sophorose					296:304	sophorose	296:304	sophorose	296:304	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	30	dep	disaccharides	270:282	arg1	maltose					332:338	maltose	332:338	maltose	332:338	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	30	dep	disaccharides	270:282	arg1	cellobiose					341:350	cellobiose	341:350	cellobiose	341:350	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	30	dep	disaccharides	270:282	arg1	disaccharides					270:282	all d-glucose-containing reducing disaccharides	236:282	all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose)	236:380	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	1	30	dep	disaccharides	270:282	arg1	nigerose					307:314	nigerose	307:314	nigerose	307:314	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	3	31	theme	1-6	753:755	arg1	bonds					757:761	1-6 bonds	753:761	1-6 bonds	753:761	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	6	32	theme	amylose	1235:1241	arg1	formation					1222:1230	the formation	1218:1230	the formation of amylose and cellulose, respectively	1218:1269	The 1-4-linked structures, involved in the formation of amylose and cellulose, respectively, were found to be advantageous due to their relatively low susceptibility to glycation.					
32413590	3	33	theme	browning	783:790	arg1	intensity					792:800	high browning intensity	778:800	high browning intensity	778:800	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	3	34	theme	formation	812:820	arg1	reactivity					822:831	oxime formation reactivity	806:831	oxime formation reactivity	806:831	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
32413590	5	35	theme	dehydrated	1160:1169	arg1	hexose					1171:1176	a highly reactive dehydrated hexose	1142:1176	a highly reactive dehydrated hexose	1142:1176	The stability of the 1-3 linkage was drastically reduced when basicity was increased due to β-elimination and the production of a highly reactive dehydrated hexose.					
32413590	4	36	theme	form	933:936	arg1	proportion					911:920	the proportion	907:920	the proportion of acyclic form	907:936	On the other hand, the proportion of acyclic form was generally very low when glucoses were linked by 1-2, 1-3 and 1-4 bonds.					
32413590	4	36	theme	form	933:936	arg1	low					957:959	low	957:959	low	957:959	On the other hand, the proportion of acyclic form was generally very low when glucoses were linked by 1-2, 1-3 and 1-4 bonds.					
32413590	2	37	theme	reported	707:714	arg1	methods					716:722	any other previously reported methods	686:722	any other previously reported methods	686:722	This new method is facile and applicable to non-labeled carbohydrates, and it is extremely sensitive, more so than any other previously reported methods.					
32413590	6	38	theme	low	1326:1328	arg1	susceptibility					1330:1343	their relatively low susceptibility	1309:1343	their relatively low susceptibility to glycation	1309:1356	The 1-4-linked structures, involved in the formation of amylose and cellulose, respectively, were found to be advantageous due to their relatively low susceptibility to glycation.					
32413590	5	39	theme	hexose	1171:1176	arg1	β-elimination					1106:1118	β-elimination	1106:1118	β-elimination	1106:1118	The stability of the 1-3 linkage was drastically reduced when basicity was increased due to β-elimination and the production of a highly reactive dehydrated hexose.					
32413590	5	39	theme	hexose	1171:1176	arg1	production					1128:1137	the production	1124:1137	the production of a highly reactive dehydrated hexose	1124:1176	The stability of the 1-3 linkage was drastically reduced when basicity was increased due to β-elimination and the production of a highly reactive dehydrated hexose.					
32413590	4	40	theme	acyclic	925:931	arg1	form					933:936	acyclic form	925:936	acyclic form	925:936	On the other hand, the proportion of acyclic form was generally very low when glucoses were linked by 1-2, 1-3 and 1-4 bonds.					
32413590	1	41	theme	oxime	521:525	arg1	formation					527:535	oxime formation	521:535	oxime formation	521:535	The susceptibility to glycation of all d-glucose-containing reducing disaccharides (kojibiose, sophorose, nigerose, laminaribiose, maltose, cellobiose, isomaltose, and gentiobiose) was evaluated by Maillard browning and the percentages of their acyclic forms estimated using a novel method to evaluate reactivity toward oxime formation were compared for the first time.					
32413590	5	42	theme	reactive	1151:1158	arg1	hexose					1171:1176	a highly reactive dehydrated hexose	1142:1176	a highly reactive dehydrated hexose	1142:1176	The stability of the 1-3 linkage was drastically reduced when basicity was increased due to β-elimination and the production of a highly reactive dehydrated hexose.					
32413590	3	43	theme	high	778:781	arg1	intensity					792:800	high browning intensity	778:800	high browning intensity	778:800	The disaccharides linked by 1-6 bonds displayed both high browning intensity and oxime formation reactivity, and they had the greatest amount of the acyclic form.					
34543843	2	0	theme	peptide	317:323	arg1	TAPBPR					333:338	TAPBPR	333:338	TAPBPR	333:338	Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR.					
34543843	2	0	theme	peptide	317:323	arg1	editor					325:330	the peptide editor	313:330	the peptide editor	313:330	Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR.					
34543843	6	1	theme	-A	813:814	arg1	*					815:815	-A*02:01	813:820	-A*02:01	813:820	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	0	2	with	interactions	64:75	arg1	TAPBPR					82:87	TAPBPR	82:87	TAPBPR	82:87	The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR.					
34543843	4	3	theme	non-glycosylated	533:548	arg1	MHC-I					550:554	non-glycosylated MHC-I	533:554	non-glycosylated MHC-I compared to their glycosylated counterparts	533:598	Subsequently, TAPBPR can dissociate peptides, even those of high affinity, more easily from non-glycosylated MHC-I compared to their glycosylated counterparts.					
34543843	7	4	theme	experimental	1064:1075	arg1	level					1077:1081	both an experimental level	1056:1081	level	1077:1081	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context.					
34543843	6	5	theme	TAPBPR-mediated	871:885	arg1	exchange					895:902	TAPBPR-mediated peptide exchange	871:902	TAPBPR-mediated peptide exchange	871:902	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	2	6	theme	class	260:264	arg1	molecules					276:284	MHC class I (MHC-I) molecules	256:284	MHC class I (MHC-I) molecules	256:284	Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR.					
34543843	6	7	theme	*	828:828	arg1	status					790:795	the glycosylation status	772:795	the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05	772:833	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	4	8	gly	glycosylated	574:585	arg1	counterparts					587:598	glycosylated counterparts	574:598	their glycosylated counterparts	568:598	Subsequently, TAPBPR can dissociate peptides, even those of high affinity, more easily from non-glycosylated MHC-I compared to their glycosylated counterparts.					
34543843	2	9	theme	MHC	256:258	arg1	class					260:264	MHC class I	256:266	MHC class I (MHC-I) molecules	256:284	Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR.					
34543843	2	9	theme	MHC	256:258	arg1	MHC-I					269:273	MHC-I	269:273	MHC-I	269:273	Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR.					
34543843	6	10	theme	-B	826:827	arg1	*					828:828	-B*27:05	826:833	-B*27:05	826:833	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	7	11	theme	biological	1092:1101	arg1	context					1103:1109	a biological context	1090:1109	a biological context	1090:1109	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context.					
34543843	1	12	theme	quality	152:158	arg1	control					160:166	quality control	152:166	quality control	152:166	Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins.					
34543843	0	13	theme	glycosylation	4:16	arg1	status					18:23	The glycosylation status	0:23	The glycosylation status of MHC class I molecules	0:48	The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR.					
34543843	2	14	theme	molecules	276:284	arg1	status					246:251	the glycosylation status	228:251	the glycosylation status of MHC class I (MHC-I) molecules	228:284	Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR.					
34543843	4	15	theme	high	501:504	arg1	affinity					506:513	high affinity	501:513	high affinity	501:513	Subsequently, TAPBPR can dissociate peptides, even those of high affinity, more easily from non-glycosylated MHC-I compared to their glycosylated counterparts.					
34543843	1	16	gly	glycoproteins	187:199	arg1	glycoproteins					187:199	glycoproteins	187:199	glycoproteins	187:199	Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins.					
34543843	7	17	with	interactions	1000:1011	arg1	TAPBPR					1018:1023	TAPBPR	1018:1023	TAPBPR	1018:1023	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context.					
34543843	7	18	dep	level	1077:1081	arg1	both					1056:1059	both	1056:1059	both	1056:1059	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context.					
34543843	5	19	theme	allosteric	659:668	arg1	release					670:676	peptide-mediated allosteric release	642:676	peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached	642:747	In addition, TAPBPR is more resistant to peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached.					
34543843	4	20	gly	non-glycosylated	533:548	arg1	MHC-I					550:554	non-glycosylated MHC-I	533:554	non-glycosylated MHC-I compared to their glycosylated counterparts	533:598	Subsequently, TAPBPR can dissociate peptides, even those of high affinity, more easily from non-glycosylated MHC-I compared to their glycosylated counterparts.					
34543843	6	21	theme	glycosylation	776:788	arg1	status					790:795	the glycosylation status	772:795	the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05	772:833	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	7	22	theme	important	1029:1037	arg1	implications					1039:1050	important implications	1029:1050	important implications	1029:1050	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context.					
34543843	1	23	dep	folding	132:138	arg1	the					128:130	the	128:130	the	128:130	Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins.					
34543843	4	24	theme	glycosylated	574:585	arg1	counterparts					587:598	glycosylated counterparts	574:598	their glycosylated counterparts	568:598	Subsequently, TAPBPR can dissociate peptides, even those of high affinity, more easily from non-glycosylated MHC-I compared to their glycosylated counterparts.					
34543843	0	25	theme	class	32:36	arg1	molecules					40:48	MHC class I molecules	28:48	MHC class I molecules	28:48	The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR.					
34543843	6	26	theme	*	815:815	arg1	status					790:795	the glycosylation status	772:795	the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05	772:833	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	7	27	contain	has	1025:1027	arg2	implications					1039:1050	important implications	1029:1050	important implications	1029:1050	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context.					
34543843	7	27	contain	has	1025:1027	arg1	discovery					909:917	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR	905:1023	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR	905:1023	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context.					
34543843	1	28	theme	glycoproteins	187:199	arg1	control					160:166	quality control	152:166	quality control	152:166	Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins.					
34543843	1	28	theme	glycoproteins	187:199	arg1	folding					132:138	folding	132:138	folding	132:138	Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins.					
34543843	1	28	theme	glycoproteins	187:199	arg1	trafficking					172:182	trafficking	172:182	trafficking	172:182	Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins.					
34543843	1	28	theme	glycoproteins	187:199	arg1	structure					141:149	structure	141:149	structure	141:149	Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins.					
34543843	0	29	theme	MHC	28:30	arg1	molecules					40:48	MHC class I molecules	28:48	MHC class I molecules	28:48	The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR.					
34543843	6	30	gly	glycosylation	776:788	arg1	*					828:828	-B*27:05	826:833	-B*27:05	826:833	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	6	30	gly	glycosylation	776:788	arg1	*					805:805	HLA-A*68:02	800:810	HLA-A*68:02	800:810	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	6	30	gly	glycosylation	776:788	arg1	*					815:815	-A*02:01	813:820	-A*02:01	813:820	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	5	31	theme	non-glycosylated	683:698	arg1	MHC-I					700:704	non-glycosylated MHC-I	683:704	non-glycosylated MHC-I	683:704	In addition, TAPBPR is more resistant to peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached.					
34543843	5	32	from	MHC-I	700:704	arg1	release					670:676	peptide-mediated allosteric release	642:676	peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached	642:747	In addition, TAPBPR is more resistant to peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached.					
34543843	0	33	gly	glycosylation	4:16	arg1	molecules					40:48	MHC class I molecules	28:48	MHC class I molecules	28:48	The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR.					
34543843	0	34	theme	molecules	40:48	arg1	status					18:23	The glycosylation status	0:23	The glycosylation status of MHC class I molecules	0:48	The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR.					
34543843	6	35	theme	peptide	887:893	arg1	exchange					895:902	TAPBPR-mediated peptide exchange	871:902	TAPBPR-mediated peptide exchange	871:902	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	0	36	theme	I	38:38	arg1	molecules					40:48	MHC class I molecules	28:48	MHC class I molecules	28:48	The glycosylation status of MHC class I molecules impacts their interactions with TAPBPR.					
34543843	5	37	from	addition	604:611	arg1	resistant					629:637	resistant	629:637	resistant	629:637	In addition, TAPBPR is more resistant to peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached.					
34543843	7	38	theme	interactions	1000:1011	arg1	affinity					982:989	the affinity	978:989	the affinity of their interactions with TAPBPR	978:1023	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context.					
34543843	1	39	theme	crucial	112:118	arg1	role					120:123	a crucial role	110:123	a crucial role	110:123	Glycosylation plays a crucial role in the folding, structure, quality control and trafficking of glycoproteins.					
34543843	6	40	theme	*	805:805	arg1	status					790:795	the glycosylation status	772:795	the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05	772:833	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	5	41	with	species	718:724	arg1	glycan					733:738	a glycan	731:738	a glycan attached	731:747	In addition, TAPBPR is more resistant to peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached.					
34543843	5	42	gly	non-glycosylated	683:698	arg1	MHC-I					700:704	non-glycosylated MHC-I	683:704	non-glycosylated MHC-I	683:704	In addition, TAPBPR is more resistant to peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached.					
34543843	5	43	theme	peptide-mediated	642:657	arg1	release					670:676	peptide-mediated allosteric release	642:676	peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached	642:747	In addition, TAPBPR is more resistant to peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached.					
34543843	6	44	theme	HLA-A	800:804	arg1	*					805:805	HLA-A*68:02	800:810	HLA-A*68:02	800:810	Consequently, we find the glycosylation status of HLA-A*68:02, -A*02:01 and -B*27:05 influences their ability to undergo TAPBPR-mediated peptide exchange.					
34543843	2	45	theme	glycosylation	232:244	arg1	status					246:251	the glycosylation status	228:251	the glycosylation status of MHC class I (MHC-I) molecules	228:284	Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR.					
34543843	7	46	attach	attached	935:942	arg1	MHC-I					947:951	MHC-I	947:951	MHC-I	947:951	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context.					
34543843	7	46	attach	attached	935:942	arg2	glycan					928:933	the glycan	924:933	the glycan attached to MHC-I	924:951	The discovery that the glycan attached to MHC-I significantly influences the affinity of their interactions with TAPBPR has important implications, on both an experimental level and in a biological context.					
34543843	2	47	gly	glycosylation	232:244	arg1	molecules					276:284	MHC class I (MHC-I) molecules	256:284	MHC class I (MHC-I) molecules	256:284	Here, we explored whether the glycosylation status of MHC class I (MHC-I) molecules impacts their affinity for the peptide editor, TAPBPR.					
34543843	5	48	from	resistant	629:637	arg1	addition					604:611	addition	604:611	addition	604:611	In addition, TAPBPR is more resistant to peptide-mediated allosteric release from non-glycosylated MHC-I compared to species with a glycan attached.					
34576979	5	0	theme	major	773:777	arg1	proteins					784:791	the major core proteins	769:791	the major core proteins for HS	769:798	The expression profiles of proteoglycans and HS-modifying enzymes was quantified in order to identify the major core proteins for HS.					
34576979	6	1	theme	important	917:925	arg1	mediators					940:948	important inflammatory mediators	917:948	important inflammatory mediators in lung pathologies	917:968	In addition, the binding affinities of human HS to two chemokines-CXCL8 and CCL2-were investigated, which represent important inflammatory mediators in lung pathologies.					
34576979	5	2	theme	proteoglycans	694:706	arg1	profiles					682:689	The expression profiles	667:689	The expression profiles of proteoglycans and HS-modifying enzymes	667:731	The expression profiles of proteoglycans and HS-modifying enzymes was quantified in order to identify the major core proteins for HS.					
34576979	8	3	theme	binding	1327:1333	arg1	affinities					1335:1344	chemokine binding affinities	1317:1344	chemokine binding affinities to various degrees	1317:1363	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	5	4	theme	core	779:782	arg1	proteins					784:791	the major core proteins	769:791	the major core proteins for HS	769:798	The expression profiles of proteoglycans and HS-modifying enzymes was quantified in order to identify the major core proteins for HS.					
34576979	4	5	theme	heparan	501:507	arg1	sulfate					509:515	heparan sulfate	501:515	heparan sulfate	501:515	In this study, heparan sulfate was isolated from human lung tissues derived from five donors and was characterized for their overall size distribution and disaccharide composition.					
34576979	1	6	theme	polysaccharides	153:167	arg1	Glycosaminoglycans					75:92	Glycosaminoglycans	75:92	Glycosaminoglycans	75:92	Glycosaminoglycans are a class of linear, highly negatively charged, O-linked polysaccharides that are involved in many (patho)physiological processes.					
34576979	1	6	theme	polysaccharides	153:167	arg1	class					100:104	a class	98:104	a class of linear, highly negatively charged, O-linked polysaccharides that are involved in many (patho)physiological processes	98:224	Glycosaminoglycans are a class of linear, highly negatively charged, O-linked polysaccharides that are involved in many (patho)physiological processes.					
34576979	8	7	theme	preparations	1295:1306	arg1	difference					1258:1267	The compositional difference	1240:1267	The compositional difference of the five human lung HS preparations	1240:1306	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	3	8	theme	human	367:371	arg1	sulfate					410:416	human, particularly organ-specific heparan sulfate	367:416	human, particularly organ-specific heparan sulfate	367:416	Structural information about human, particularly organ-specific heparan sulfate, and how it compares with HS from other organisms, is very limited.					
34576979	7	9	dep	sex	1134:1136	arg1	discovered					1202:1211	discovered	1202:1211	was accidentally discovered to contain a solid tumor	1185:1236	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	9	10	theme	immunological	1591:1603	arg1	disorders					1605:1613	lung immunological disorders	1586:1613	lung immunological disorders	1586:1613	This represents important new insights that could be translated into novel therapeutic concepts for individually treating lung immunological disorders via HS targets.					
34576979	8	11	theme	selective	1377:1385	arg1	responses					1399:1407	selective immune cell responses	1377:1407	selective immune cell responses	1377:1407	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	5	12	theme	HS-modifying	712:723	arg1	enzymes					725:731	HS-modifying enzymes	712:731	HS-modifying enzymes	712:731	The expression profiles of proteoglycans and HS-modifying enzymes was quantified in order to identify the major core proteins for HS.					
34576979	9	13	theme	lung	1586:1589	arg1	disorders					1605:1613	lung immunological disorders	1586:1613	lung immunological disorders	1586:1613	This represents important new insights that could be translated into novel therapeutic concepts for individually treating lung immunological disorders via HS targets.					
34576979	3	14	dep	limited	477:483	arg1	compares					430:437	compares	430:437	compares with HS from other organisms	430:466	Structural information about human, particularly organ-specific heparan sulfate, and how it compares with HS from other organisms, is very limited.					
34576979	9	15	theme	novel	1533:1537	arg1	concepts					1551:1558	novel therapeutic concepts	1533:1558	novel therapeutic concepts for individually treating lung immunological disorders via HS targets	1533:1628	This represents important new insights that could be translated into novel therapeutic concepts for individually treating lung immunological disorders via HS targets.					
34576979	7	16	theme	lungs	1179:1183	arg1	lungs					1179:1183	the donor lungs	1169:1183	the donor lungs	1169:1183	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	7	16	theme	lungs	1179:1183	arg1	one					1162:1164	one	1162:1164	one	1162:1164	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	8	17	theme	human	1281:1285	arg1	preparations					1295:1306	the five human lung HS preparations	1272:1306	the five human lung HS preparations	1272:1306	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	8	18	theme	compositional	1244:1256	arg1	difference					1258:1267	The compositional difference	1240:1267	The compositional difference of the five human lung HS preparations	1240:1306	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	1	19	dep	linear	109:114	arg1	O-linked					144:151	O-linked	144:151	O-linked	144:151	Glycosaminoglycans are a class of linear, highly negatively charged, O-linked polysaccharides that are involved in many (patho)physiological processes.					
34576979	1	19	dep	linear	109:114	arg1	charged					135:141	charged	135:141	charged	135:141	Glycosaminoglycans are a class of linear, highly negatively charged, O-linked polysaccharides that are involved in many (patho)physiological processes.					
34576979	8	20	theme	immune	1387:1392	arg1	responses					1399:1407	selective immune cell responses	1377:1407	selective immune cell responses	1377:1407	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	9	21	theme	new	1490:1492	arg1	insights					1494:1501	important new insights	1480:1501	important new insights that could be translated into novel therapeutic concepts for individually treating lung immunological disorders via HS targets	1480:1628	This represents important new insights that could be translated into novel therapeutic concepts for individually treating lung immunological disorders via HS targets.					
34576979	8	22	theme	various	1349:1355	arg1	degrees					1357:1363	various degrees	1349:1363	various degrees	1349:1363	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	6	23	theme	HS	846:847	arg1	affinities					826:835	the binding affinities	814:835	the binding affinities of human HS to two chemokines-CXCL8 and CCL2-were	814:885	In addition, the binding affinities of human HS to two chemokines-CXCL8 and CCL2-were investigated, which represent important inflammatory mediators in lung pathologies.					
34576979	2	24	theme	such	267:270	arg1	processes					272:280	such processes	267:280	such processes	267:280	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	1	25	theme	patho	196:200	arg1	processes					216:224	many (patho)physiological processes	190:224	many (patho)physiological processes	190:224	Glycosaminoglycans are a class of linear, highly negatively charged, O-linked polysaccharides that are involved in many (patho)physiological processes.					
34576979	4	26	theme	disaccharide	641:652	arg1	composition					654:664	disaccharide composition	641:664	disaccharide composition	641:664	In this study, heparan sulfate was isolated from human lung tissues derived from five donors and was characterized for their overall size distribution and disaccharide composition.					
34576979	3	27	theme	heparan	402:408	arg1	sulfate					410:416	human, particularly organ-specific heparan sulfate	367:416	human, particularly organ-specific heparan sulfate	367:416	Structural information about human, particularly organ-specific heparan sulfate, and how it compares with HS from other organisms, is very limited.					
34576979	7	28	theme	donor	1173:1177	arg1	lungs					1179:1183	the donor lungs	1169:1183	the donor lungs	1169:1183	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	4	29	theme	lung	541:544	arg1	tissues					546:552	human lung tissues	535:552	human lung tissues derived from five donors	535:577	In this study, heparan sulfate was isolated from human lung tissues derived from five donors and was characterized for their overall size distribution and disaccharide composition.					
34576979	9	30	theme	HS	1619:1620	arg1	targets					1622:1628	HS targets	1619:1628	HS targets	1619:1628	This represents important new insights that could be translated into novel therapeutic concepts for individually treating lung immunological disorders via HS targets.					
34576979	9	31	theme	important	1480:1488	arg1	insights					1494:1501	important new insights	1480:1501	important new insights that could be translated into novel therapeutic concepts for individually treating lung immunological disorders via HS targets	1480:1628	This represents important new insights that could be translated into novel therapeutic concepts for individually treating lung immunological disorders via HS targets.					
34576979	4	32	theme	human	535:539	arg1	tissues					546:552	human lung tissues	535:552	human lung tissues derived from five donors	535:577	In this study, heparan sulfate was isolated from human lung tissues derived from five donors and was characterized for their overall size distribution and disaccharide composition.					
34576979	2	33	theme	heparan	316:322	arg1	HS					333:334	HS	333:334	HS	333:334	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	2	33	theme	heparan	316:322	arg1	sulfate					324:330	porcine-derived heparan sulfate	300:330	porcine-derived heparan sulfate (HS)	300:335	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	1	34	theme	many	190:193	arg1	processes					216:224	many (patho)physiological processes	190:224	many (patho)physiological processes	190:224	Glycosaminoglycans are a class of linear, highly negatively charged, O-linked polysaccharides that are involved in many (patho)physiological processes.					
34576979	2	35	theme	processes	272:280	arg1	investigations					249:262	In vitro experimental investigations	227:262	In vitro experimental investigations of such processes	227:280	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	2	36	theme	porcine-derived	300:314	arg1	HS					333:334	HS	333:334	HS	333:334	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	2	36	theme	porcine-derived	300:314	arg1	sulfate					324:330	porcine-derived heparan sulfate	300:330	porcine-derived heparan sulfate (HS)	300:335	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	4	37	theme	overall	611:617	arg1	distribution					624:635	their overall size distribution	605:635	their overall size distribution	605:635	In this study, heparan sulfate was isolated from human lung tissues derived from five donors and was characterized for their overall size distribution and disaccharide composition.					
34576979	0	38	theme	Sulfate	42:48	arg1	Characterization					14:29	Characterization	14:29	Characterization	14:29	Isolation and Characterization of Heparan Sulfate from Human Lung Tissues.					
34576979	0	38	theme	Sulfate	42:48	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and Characterization of Heparan Sulfate from Human Lung Tissues.					
34576979	8	39	theme	chemokine	1317:1325	arg1	affinities					1335:1344	chemokine binding affinities	1317:1344	chemokine binding affinities to various degrees	1317:1363	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	5	40	theme	enzymes	725:731	arg1	profiles					682:689	The expression profiles	667:689	The expression profiles of proteoglycans and HS-modifying enzymes	667:731	The expression profiles of proteoglycans and HS-modifying enzymes was quantified in order to identify the major core proteins for HS.					
34576979	2	41	dep	In	227:228	arg1	vitro					230:234	vitro	230:234	vitro	230:234	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	2	42	theme	In	227:228	arg1	investigations					249:262	In vitro experimental investigations	227:262	In vitro experimental investigations of such processes	227:280	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	3	43	theme	other	452:456	arg1	organisms					458:466	other organisms	452:466	other organisms	452:466	Structural information about human, particularly organ-specific heparan sulfate, and how it compares with HS from other organisms, is very limited.					
34576979	0	44	theme	Heparan	34:40	arg1	Sulfate					42:48	Heparan Sulfate	34:48	Heparan Sulfate	34:48	Isolation and Characterization of Heparan Sulfate from Human Lung Tissues.					
34576979	7	45	theme	disaccharide	1071:1082	arg1	composition					1084:1094	the disaccharide composition	1067:1094	the disaccharide composition	1067:1094	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	0	46	from	Tissues	66:72	arg1	Characterization					14:29	Characterization	14:29	Characterization	14:29	Isolation and Characterization of Heparan Sulfate from Human Lung Tissues.					
34576979	0	46	from	Tissues	66:72	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and Characterization of Heparan Sulfate from Human Lung Tissues.					
34576979	8	47	theme	cell	1394:1397	arg1	responses					1399:1407	selective immune cell responses	1377:1407	selective immune cell responses	1377:1407	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	0	48	theme	Human	55:59	arg1	Tissues					66:72	Human Lung Tissues	55:72	Human Lung Tissues	55:72	Isolation and Characterization of Heparan Sulfate from Human Lung Tissues.					
34576979	2	49	link	porcine-derived	300:314	arg1	HS					333:334	HS	333:334	HS	333:334	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	2	49	link	porcine-derived	300:314	arg1	sulfate					324:330	porcine-derived heparan sulfate	300:330	porcine-derived heparan sulfate (HS)	300:335	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	5	50	theme	expression	671:680	arg1	profiles					682:689	The expression profiles	667:689	The expression profiles of proteoglycans and HS-modifying enzymes	667:731	The expression profiles of proteoglycans and HS-modifying enzymes was quantified in order to identify the major core proteins for HS.					
34576979	3	51	theme	Structural	338:347	arg1	information					349:359	Structural information	338:359	Structural information about human, particularly organ-specific heparan sulfate	338:416	Structural information about human, particularly organ-specific heparan sulfate, and how it compares with HS from other organisms, is very limited.					
34576979	9	52	dep	novel	1533:1537	arg1	therapeutic					1539:1549	therapeutic	1539:1549	therapeutic	1539:1549	This represents important new insights that could be translated into novel therapeutic concepts for individually treating lung immunological disorders via HS targets.					
34576979	3	53	from	organisms	458:466	arg1	HS					444:445	HS	444:445	HS from other organisms	444:466	Structural information about human, particularly organ-specific heparan sulfate, and how it compares with HS from other organisms, is very limited.					
34576979	1	54	theme	linear	109:114	arg1	polysaccharides					153:167	linear, highly negatively charged, O-linked polysaccharides	109:167	linear, highly negatively charged, O-linked polysaccharides that are involved in many (patho)physiological processes	109:224	Glycosaminoglycans are a class of linear, highly negatively charged, O-linked polysaccharides that are involved in many (patho)physiological processes.					
34576979	7	55	theme	health	1148:1153	arg1	stage					1155:1159	health stage	1148:1159	health stage	1148:1159	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	7	56	from	class	1037:1041	arg1	lungs					1052:1056	human lungs	1046:1056	human lungs	1046:1056	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	6	57	from	mediators	940:948	arg1	pathologies					958:968	lung pathologies	953:968	lung pathologies	953:968	In addition, the binding affinities of human HS to two chemokines-CXCL8 and CCL2-were investigated, which represent important inflammatory mediators in lung pathologies.					
34576979	6	58	theme	human	840:844	arg1	HS					846:847	human HS	840:847	human HS	840:847	In addition, the binding affinities of human HS to two chemokines-CXCL8 and CCL2-were investigated, which represent important inflammatory mediators in lung pathologies.					
34576979	7	59	theme	predominant	1012:1022	arg1	class					1037:1041	the predominant proteoglycan class	1008:1041	the predominant proteoglycan class in human lungs	1008:1056	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	7	59	theme	predominant	1012:1022	arg1	syndecans					994:1002	syndecans	994:1002	syndecans	994:1002	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	3	60	dep	human	367:371	arg1	organ-specific					387:400	organ-specific	387:400	organ-specific	387:400	Structural information about human, particularly organ-specific heparan sulfate, and how it compares with HS from other organisms, is very limited.					
34576979	8	61	theme	relative	1426:1433	arg1	affinities					1452:1461	the relative chemokine-glycan affinities	1422:1461	the relative chemokine-glycan affinities	1422:1461	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	7	62	theme	proteoglycan	1024:1035	arg1	class					1037:1041	the predominant proteoglycan class	1008:1041	the predominant proteoglycan class in human lungs	1008:1056	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	7	62	theme	proteoglycan	1024:1035	arg1	syndecans					994:1002	syndecans	994:1002	syndecans	994:1002	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	8	63	theme	HS	1292:1293	arg1	preparations					1295:1306	the five human lung HS preparations	1272:1306	the five human lung HS preparations	1272:1306	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	4	64	attach	isolated	521:528	arg2	sulfate					509:515	heparan sulfate	501:515	heparan sulfate	501:515	In this study, heparan sulfate was isolated from human lung tissues derived from five donors and was characterized for their overall size distribution and disaccharide composition.					
34576979	4	64	attach	isolated	521:528	arg1	tissues					546:552	human lung tissues	535:552	human lung tissues derived from five donors	535:577	In this study, heparan sulfate was isolated from human lung tissues derived from five donors and was characterized for their overall size distribution and disaccharide composition.					
34576979	1	65	theme	physiological	202:214	arg1	processes					216:224	many (patho)physiological processes	190:224	many (patho)physiological processes	190:224	Glycosaminoglycans are a class of linear, highly negatively charged, O-linked polysaccharides that are involved in many (patho)physiological processes.					
34576979	4	66	attach	derived	554:560	arg1	donors					572:577	five donors	567:577	five donors	567:577	In this study, heparan sulfate was isolated from human lung tissues derived from five donors and was characterized for their overall size distribution and disaccharide composition.					
34576979	4	66	attach	derived	554:560	arg2	tissues					546:552	human lung tissues	535:552	human lung tissues derived from five donors	535:577	In this study, heparan sulfate was isolated from human lung tissues derived from five donors and was characterized for their overall size distribution and disaccharide composition.					
34576979	7	67	contain	contain	1216:1222	arg2	tumor					1232:1236	a solid tumor	1224:1236	a solid tumor	1224:1236	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	7	67	contain	contain	1216:1222	arg1	lungs					1179:1183	the donor lungs	1169:1183	the donor lungs	1169:1183	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	7	67	contain	contain	1216:1222	arg1	one					1162:1164	one	1162:1164	one	1162:1164	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	8	68	theme	lung	1287:1290	arg1	preparations					1295:1306	the five human lung HS preparations	1272:1306	the five human lung HS preparations	1272:1306	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	6	69	theme	binding	818:824	arg1	affinities					826:835	the binding affinities	814:835	the binding affinities of human HS to two chemokines-CXCL8 and CCL2-were	814:885	In addition, the binding affinities of human HS to two chemokines-CXCL8 and CCL2-were investigated, which represent important inflammatory mediators in lung pathologies.					
34576979	2	70	theme	experimental	236:247	arg1	investigations					249:262	In vitro experimental investigations	227:262	In vitro experimental investigations of such processes	227:280	In vitro experimental investigations of such processes typically involve porcine-derived heparan sulfate (HS).					
34576979	4	71	theme	size	619:622	arg1	distribution					624:635	their overall size distribution	605:635	their overall size distribution	605:635	In this study, heparan sulfate was isolated from human lung tissues derived from five donors and was characterized for their overall size distribution and disaccharide composition.					
34576979	7	72	theme	solid	1226:1230	arg1	tumor					1232:1236	a solid tumor	1224:1236	a solid tumor	1224:1236	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	0	73	theme	Lung	61:64	arg1	Tissues					66:72	Human Lung Tissues	55:72	Human Lung Tissues	55:72	Isolation and Characterization of Heparan Sulfate from Human Lung Tissues.					
34576979	6	74	theme	inflammatory	927:938	arg1	mediators					940:948	important inflammatory mediators	917:948	important inflammatory mediators in lung pathologies	917:968	In addition, the binding affinities of human HS to two chemokines-CXCL8 and CCL2-were investigated, which represent important inflammatory mediators in lung pathologies.					
34576979	8	75	theme	chemokine-glycan	1435:1450	arg1	affinities					1452:1461	the relative chemokine-glycan affinities	1422:1461	the relative chemokine-glycan affinities	1422:1461	The compositional difference of the five human lung HS preparations affected chemokine binding affinities to various degrees, indicating selective immune cell responses depending on the relative chemokine-glycan affinities.					
34576979	7	76	theme	human	1046:1050	arg1	lungs					1052:1056	human lungs	1046:1056	human lungs	1046:1056	Our data revealed that syndecans are the predominant proteoglycan class in human lungs and that the disaccharide composition varies among individuals according to sex, age, and health stage (one of the donor lungs was accidentally discovered to contain a solid tumor).					
34576979	6	77	theme	lung	953:956	arg1	pathologies					958:968	lung pathologies	953:968	lung pathologies	953:968	In addition, the binding affinities of human HS to two chemokines-CXCL8 and CCL2-were investigated, which represent important inflammatory mediators in lung pathologies.					
33564762	1	0	theme	surface	244:250	arg1	proteins					252:259	viral surface proteins	238:259	viral surface proteins with tolerogenic host-like structures	238:297	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	6	1	theme	amino	1116:1120	arg1	acids					1122:1126	three amino acids	1110:1126	three amino acids from known N-glycosites	1110:1150	Strikingly, 16 out of 25 O-glycosites were located within three amino acids from known N-glycosites.					
33564762	6	2	located	located	1095:1101	arg1	acids					1122:1126	three amino acids	1110:1126	three amino acids from known N-glycosites	1110:1150	Strikingly, 16 out of 25 O-glycosites were located within three amino acids from known N-glycosites.					
33564762	6	2	located	located	1095:1101	arg2	O-glycosites					1077:1088	16 out of 25 O-glycosites	1064:1088	16 out of 25 O-glycosites	1064:1088	Strikingly, 16 out of 25 O-glycosites were located within three amino acids from known N-glycosites.					
33564762	8	3	theme	complementary	1309:1321	arg1	functions					1323:1331	possible complementary functions	1300:1331	possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2	1300:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	3	4	theme	large	513:517	arg1	family					519:524	a large family	511:524	a large family of initiating enzymes with variable expression in cells and tissues	511:592	The location of O-glycans is controlled by a large family of initiating enzymes with variable expression in cells and tissues and hence difficult to predict.					
33564762	8	5	from	shielding	1356:1364	arg1	design					1427:1432	subunit vaccine design	1411:1432	subunit vaccine design for SARS-CoV-2	1411:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	4	6	theme	human	807:811	arg1	ectodomains					827:837	human cell-produced ectodomains	807:837	human cell-produced ectodomains	807:837	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	2	7	theme	protein	319:325	arg1	S					327:327	SARS-CoV-2 surface protein S	300:327	SARS-CoV-2 surface protein S	300:327	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.					
33564762	1	8	theme	glycosylation	182:194	arg1	machinery					196:204	its glycosylation machinery	178:204	its glycosylation machinery	178:204	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	4	9	used	used	635:638	arg2	we					632:633	we	632:633	we	632:633	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	8	10	theme	possible	1300:1307	arg1	functions					1323:1331	possible complementary functions	1300:1331	possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2	1300:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	2	11	theme	SARS-CoV-2	300:309	arg1	S					327:327	SARS-CoV-2 surface protein S	300:327	SARS-CoV-2 surface protein S	300:327	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.					
33564762	8	12	theme	O-glycosylation	1392:1406	arg1	shielding					1356:1364	immune shielding	1349:1364	immune shielding	1349:1364	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	8	12	theme	O-glycosylation	1392:1406	arg1	effects					1381:1387	negligible effects	1370:1387	negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2	1370:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	4	13	theme	receptor	863:870	arg1	RBD					888:890	RBD	888:890	RBD	888:890	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	13	theme	receptor	863:870	arg1	domain					880:885	receptor binding domain	863:885	receptor binding domain (RBD)	863:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	8	14	theme	subunit	1411:1417	arg1	design					1427:1432	subunit vaccine design	1411:1432	subunit vaccine design for SARS-CoV-2	1411:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	7	15	contain	had	1259:1261	arg2	occupancy					1275:1283	low overall occupancy	1263:1283	low overall occupancy	1263:1283	However, O-glycosylation was primarily found on peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy.					
33564762	7	15	contain	had	1259:1261	arg1	peptides					1201:1208	peptides	1201:1208	peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy	1201:1283	However, O-glycosylation was primarily found on peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy.					
33564762	3	16	theme	variable	553:560	arg1	expression					562:571	variable expression	553:571	variable expression in cells and tissues	553:592	The location of O-glycans is controlled by a large family of initiating enzymes with variable expression in cells and tissues and hence difficult to predict.					
33564762	4	17	from	entities	768:775	arg1	positions					707:715	the precise positions	695:715	the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	695:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	1	18	theme	translation	146:156	arg1	processes					158:166	the host translation processes	137:166	the host translation processes	137:166	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	4	19	theme	O-glycoproteomic	661:676	arg1	workflows					678:686	our well-established O-glycoproteomic workflows	640:686	our well-established O-glycoproteomic workflows	640:686	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	2	20	link	N-linked	407:414	arg1	glycans					416:422	N-linked glycans	407:422	N-linked glycans	407:422	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.					
33564762	4	21	theme	sites	743:747	arg1	positions					707:715	the precise positions	695:715	the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	695:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	22	from	sites	743:747	arg1	cell					799:802	insect cell	792:802	insect cell	792:802	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	22	from	sites	743:747	arg1	entities					768:775	three different entities	752:775	three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	752:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	22	from	sites	743:747	arg1	cell					850:853	insect cell	843:853	insect cell derived receptor binding domain (RBD)	843:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	5	23	gly	O-glycosites	906:917	arg2	O-glycosites					906:917	25 O-glycosites	903:917	25 O-glycosites	903:917	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	1	24	dep	hijack	121:126	arg1	cover					232:236	cover	232:236	cover viral surface proteins with tolerogenic host-like structures	232:297	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	8	25	from	design	1427:1432	arg1	functions					1323:1331	possible complementary functions	1300:1331	possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2	1300:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	5	26	theme	RBD	1047:1049	arg1	RBD					1047:1049	the monomeric RBD	1033:1049	the monomeric RBD	1033:1049	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	5	26	theme	RBD	1047:1049	arg1	ectodomains					969:979	the two ectodomains	961:979	the two ectodomains of different cell origin	961:1004	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	5	26	theme	RBD	1047:1049	arg1	pattern					1022:1028	a distinct pattern	1011:1028	a distinct pattern of the monomeric RBD	1011:1049	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	5	26	theme	RBD	1047:1049	arg1	origin					999:1004	different cell origin	984:1004	different cell origin	984:1004	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	7	27	theme	overall	1267:1273	arg1	occupancy					1275:1283	low overall occupancy	1263:1283	low overall occupancy	1263:1283	However, O-glycosylation was primarily found on peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy.					
33564762	0	28	theme	insect	79:84	arg1	cells					96:100	insect and human cells	79:100	insect and human cells	79:100	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells.					
33564762	7	29	gly	O-glycosylation	1162:1176	arg2	peptides					1201:1208	peptides	1201:1208	peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy	1201:1283	However, O-glycosylation was primarily found on peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy.					
33564762	7	29	gly	O-glycosylation	1162:1176	arg1	peptides					1201:1208	peptides	1201:1208	peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy	1201:1283	However, O-glycosylation was primarily found on peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy.					
33564762	0	30	theme	Site-specific	0:12	arg1	O-glycosylation					14:28	Site-specific O-glycosylation	0:28	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein	0:65	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells.					
33564762	1	31	theme	tolerogenic	266:276	arg1	structures					288:297	tolerogenic host-like structures	266:297	tolerogenic host-like structures	266:297	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	4	32	from	positions	707:715	arg1	cell					799:802	insect cell	792:802	insect cell	792:802	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	32	from	positions	707:715	arg1	entities					768:775	three different entities	752:775	three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	752:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	32	from	positions	707:715	arg1	cell					850:853	insect cell	843:853	insect cell derived receptor binding domain (RBD)	843:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	2	33	theme	glycans	416:422	arg1	shield					397:402	shield	397:402	shield	397:402	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.					
33564762	4	34	theme	O-linked	720:727	arg1	sites					743:747	O-linked glycosylation sites	720:747	O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	720:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	3	35	with	enzymes	540:546	arg1	expression					562:571	variable expression	553:571	variable expression in cells and tissues	553:592	The location of O-glycans is controlled by a large family of initiating enzymes with variable expression in cells and tissues and hence difficult to predict.					
33564762	0	36	theme	SARS-CoV-2	42:51	arg1	protein					59:65	SARS-CoV-2 spike protein	42:65	SARS-CoV-2 spike protein	42:65	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells.					
33564762	0	37	gly	O-glycosylation	14:28	arg1	protein					59:65	SARS-CoV-2 spike protein	42:65	SARS-CoV-2 spike protein	42:65	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells.					
33564762	1	38	with	proteins	252:259	arg1	structures					288:297	tolerogenic host-like structures	266:297	tolerogenic host-like structures	266:297	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	2	39	theme	few	431:433	arg1	O-glycosites					435:446	a few O-glycosites	429:446	a few O-glycosites	429:446	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.					
33564762	0	40	theme	protein	59:65	arg1	analysis					30:37	Site-specific O-glycosylation analysis	0:37	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein	0:65	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells.					
33564762	5	41	theme	different	984:992	arg1	origin					999:1004	different cell origin	984:1004	different cell origin	984:1004	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	8	42	theme	negligible	1370:1379	arg1	effects					1381:1387	negligible effects	1370:1387	negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2	1370:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	4	43	theme	S	788:788	arg1	cell					799:802	insect cell	792:802	insect cell	792:802	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	43	theme	S	788:788	arg1	entities					768:775	three different entities	752:775	three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	752:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	43	theme	S	788:788	arg1	cell					850:853	insect cell	843:853	insect cell derived receptor binding domain (RBD)	843:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	44	theme	protein	780:786	arg1	S					788:788	protein S	780:788	protein S	780:788	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	3	45	theme	O-glycans	484:492	arg1	location					472:479	The location	468:479	The location of O-glycans	468:492	The location of O-glycans is controlled by a large family of initiating enzymes with variable expression in cells and tissues and hence difficult to predict.					
33564762	8	46	from	functions	1323:1331	arg1	shielding					1356:1364	immune shielding	1349:1364	immune shielding	1349:1364	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	8	46	from	functions	1323:1331	arg1	effects					1381:1387	negligible effects	1370:1387	negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2	1370:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	8	46	from	functions	1323:1331	arg1	design					1427:1432	subunit vaccine design	1411:1432	subunit vaccine design for SARS-CoV-2	1411:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	1	47	theme	viral	238:242	arg1	proteins					252:259	viral surface proteins	238:259	viral surface proteins with tolerogenic host-like structures	238:297	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	4	48	theme	insect	843:848	arg1	entities					768:775	three different entities	752:775	three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	752:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	48	theme	insect	843:848	arg1	cell					850:853	insect cell	843:853	insect cell derived receptor binding domain (RBD)	843:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	2	49	from	trimer	343:348	arg1	surface					363:369	the viral surface	353:369	the viral surface	353:369	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.					
33564762	8	50	gly	O-glycosylation	1392:1406	arg1	subunit					1411:1417	subunit vaccine design	1411:1432	subunit vaccine design for SARS-CoV-2	1411:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	8	50	gly	O-glycosylation	1392:1406	arg1	vaccine					1419:1425	subunit vaccine design	1411:1432	subunit vaccine design for SARS-CoV-2	1411:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	6	51	gly	O-glycosites	1077:1088	arg2	O-glycosites					1077:1088	16 out of 25 O-glycosites	1064:1088	16 out of 25 O-glycosites	1064:1088	Strikingly, 16 out of 25 O-glycosites were located within three amino acids from known N-glycosites.					
33564762	3	52	theme	initiating	529:538	arg1	enzymes					540:546	initiating enzymes	529:546	initiating enzymes with variable expression in cells and tissues	529:592	The location of O-glycans is controlled by a large family of initiating enzymes with variable expression in cells and tissues and hence difficult to predict.					
33564762	5	53	from	patterns	949:956	arg1	RBD					1047:1049	the monomeric RBD	1033:1049	the monomeric RBD	1033:1049	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	5	53	from	patterns	949:956	arg1	ectodomains					969:979	the two ectodomains	961:979	the two ectodomains of different cell origin	961:1004	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	5	53	from	patterns	949:956	arg1	pattern					1022:1028	a distinct pattern	1011:1028	a distinct pattern of the monomeric RBD	1011:1049	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	5	53	from	patterns	949:956	arg1	origin					999:1004	different cell origin	984:1004	different cell origin	984:1004	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	4	54	dep	entities	768:775	arg1	ectodomains					827:837	human cell-produced ectodomains	807:837	human cell-produced ectodomains	807:837	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	54	dep	entities	768:775	arg1	cell					799:802	insect cell	792:802	insect cell	792:802	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	54	dep	entities	768:775	arg1	entities					768:775	three different entities	752:775	three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	752:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	54	dep	entities	768:775	arg1	cell					850:853	insect cell	843:853	insect cell derived receptor binding domain (RBD)	843:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	55	theme	cell-produced	813:825	arg1	ectodomains					827:837	human cell-produced ectodomains	807:837	human cell-produced ectodomains	807:837	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	8	56	theme	immune	1349:1354	arg1	shielding					1356:1364	immune shielding	1349:1364	immune shielding	1349:1364	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	2	57	theme	surface	311:317	arg1	S					327:327	SARS-CoV-2 surface protein S	300:327	SARS-CoV-2 surface protein S	300:327	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.					
33564762	8	58	from	effects	1381:1387	arg1	design					1427:1432	subunit vaccine design	1411:1432	subunit vaccine design for SARS-CoV-2	1411:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	3	59	theme	enzymes	540:546	arg1	family					519:524	a large family	511:524	a large family of initiating enzymes with variable expression in cells and tissues	511:592	The location of O-glycans is controlled by a large family of initiating enzymes with variable expression in cells and tissues and hence difficult to predict.					
33564762	5	60	theme	distinct	1013:1020	arg1	pattern					1022:1028	a distinct pattern	1011:1028	a distinct pattern of the monomeric RBD	1011:1049	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	5	61	theme	cell	994:997	arg1	origin					999:1004	different cell origin	984:1004	different cell origin	984:1004	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	8	62	theme	vaccine	1419:1425	arg1	design					1427:1432	subunit vaccine design	1411:1432	subunit vaccine design for SARS-CoV-2	1411:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	5	63	theme	origin	999:1004	arg1	RBD					1047:1049	the monomeric RBD	1033:1049	the monomeric RBD	1033:1049	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	5	63	theme	origin	999:1004	arg1	ectodomains					969:979	the two ectodomains	961:979	the two ectodomains of different cell origin	961:1004	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	5	63	theme	origin	999:1004	arg1	pattern					1022:1028	a distinct pattern	1011:1028	a distinct pattern of the monomeric RBD	1011:1049	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	5	63	theme	origin	999:1004	arg1	origin					999:1004	different cell origin	984:1004	different cell origin	984:1004	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	4	64	theme	binding	872:878	arg1	RBD					888:890	RBD	888:890	RBD	888:890	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	64	theme	binding	872:878	arg1	domain					880:885	receptor binding domain	863:885	receptor binding domain (RBD)	863:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	6	65	theme	known	1133:1137	arg1	N-glycosites					1139:1150	known N-glycosites	1133:1150	known N-glycosites	1133:1150	Strikingly, 16 out of 25 O-glycosites were located within three amino acids from known N-glycosites.					
33564762	4	66	gly	glycosylation	729:741	arg2	sites					743:747	O-linked glycosylation sites	720:747	O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	720:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	1	67	theme	host	141:144	arg1	processes					158:166	the host translation processes	137:166	the host translation processes	137:166	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	0	68	theme	human	90:94	arg1	cells					96:100	insect and human cells	79:100	insect and human cells	79:100	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells.					
33564762	7	69	located	found	1192:1196	arg2	O-glycosylation					1162:1176	O-glycosylation	1162:1176	O-glycosylation	1162:1176	However, O-glycosylation was primarily found on peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy.					
33564762	7	69	located	found	1192:1196	arg1	peptides					1201:1208	peptides	1201:1208	peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy	1201:1283	However, O-glycosylation was primarily found on peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy.					
33564762	4	70	theme	well-established	644:659	arg1	workflows					678:686	our well-established O-glycoproteomic workflows	640:686	our well-established O-glycoproteomic workflows	640:686	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	6	71	from	N-glycosites	1139:1150	arg1	acids					1122:1126	three amino acids	1110:1126	three amino acids from known N-glycosites	1110:1150	Strikingly, 16 out of 25 O-glycosites were located within three amino acids from known N-glycosites.					
33564762	4	72	theme	glycosylation	729:741	arg1	sites					743:747	O-linked glycosylation sites	720:747	O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	720:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	5	73	theme	monomeric	1037:1045	arg1	RBD					1047:1049	the monomeric RBD	1033:1049	the monomeric RBD	1033:1049	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	7	74	theme	low	1263:1265	arg1	occupancy					1275:1283	low overall occupancy	1263:1283	low overall occupancy	1263:1283	However, O-glycosylation was primarily found on peptides that were unoccupied by N-glycans, and otherwise had low overall occupancy.					
33564762	0	75	theme	O-glycosylation	14:28	arg1	analysis					30:37	Site-specific O-glycosylation analysis	0:37	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein	0:65	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells.					
33564762	4	76	theme	precise	699:705	arg1	positions					707:715	the precise positions	695:715	the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	695:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	1	77	theme	host-like	278:286	arg1	structures					288:297	tolerogenic host-like structures	266:297	tolerogenic host-like structures	266:297	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	1	78	theme	Enveloped	103:111	arg1	viruses					113:119	Enveloped viruses	103:119	Enveloped viruses	103:119	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	2	79	theme	N-linked	407:414	arg1	glycans					416:422	N-linked glycans	407:422	N-linked glycans	407:422	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.					
33564762	3	80	from	expression	562:571	arg1	tissues					586:592	tissues	586:592	tissues	586:592	The location of O-glycans is controlled by a large family of initiating enzymes with variable expression in cells and tissues and hence difficult to predict.					
33564762	3	80	from	expression	562:571	arg1	cells					576:580	cells	576:580	cells	576:580	The location of O-glycans is controlled by a large family of initiating enzymes with variable expression in cells and tissues and hence difficult to predict.					
33564762	0	81	theme	spike	53:57	arg1	protein					59:65	SARS-CoV-2 spike protein	42:65	SARS-CoV-2 spike protein	42:65	Site-specific O-glycosylation analysis of SARS-CoV-2 spike protein produced in insect and human cells.					
33564762	2	82	gly	O-glycosites	435:446	arg2	O-glycosites					435:446	a few O-glycosites	429:446	a few O-glycosites	429:446	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.					
33564762	8	83	theme	O-glycans	1336:1344	arg1	functions					1323:1331	possible complementary functions	1300:1331	possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2	1300:1447	This suggests possible complementary functions of O-glycans in immune shielding and negligible effects of O-glycosylation on subunit vaccine design for SARS-CoV-2.					
33564762	4	84	theme	insect	792:797	arg1	entities					768:775	three different entities	752:775	three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	752:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	84	theme	insect	792:797	arg1	cell					799:802	insect cell	792:802	insect cell	792:802	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	5	85	theme	similar	941:947	arg1	patterns					949:956	similar patterns	941:956	similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD	941:1049	In total 25 O-glycosites were identified, with similar patterns in the two ectodomains of different cell origin, and a distinct pattern of the monomeric RBD.					
33564762	4	86	theme	different	758:766	arg1	cell					799:802	insect cell	792:802	insect cell	792:802	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	86	theme	different	758:766	arg1	entities					768:775	three different entities	752:775	three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	752:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	4	86	theme	different	758:766	arg1	cell					850:853	insect cell	843:853	insect cell derived receptor binding domain (RBD)	843:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	1	87	theme	variable	216:223	arg1	extent					225:230	a variable extent	214:230	a variable extent	214:230	Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures.					
33564762	4	88	link	O-linked	720:727	arg1	sites					743:747	O-linked glycosylation sites	720:747	O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD)	720:891	Here, we used our well-established O-glycoproteomic workflows to map the precise positions of O-linked glycosylation sites on three different entities of protein S - insect cell or human cell-produced ectodomains, or insect cell derived receptor binding domain (RBD).					
33564762	6	89	gly	N-glycosites	1139:1150	arg2	N-glycosites					1139:1150	known N-glycosites	1133:1150	known N-glycosites	1133:1150	Strikingly, 16 out of 25 O-glycosites were located within three amino acids from known N-glycosites.					
33564762	2	90	theme	viral	357:361	arg1	surface					363:369	the viral surface	353:369	the viral surface	353:369	SARS-CoV-2 surface protein S presents as a trimer on the viral surface and is covered by a dense shield of N-linked glycans, and a few O-glycosites have been reported.					
33914359	0	0	theme	Efficient	83:91	arg1	Generation					93:102	Efficient Generation	83:102	Efficient Generation of Transglycosylases	83:123	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.					
33914359	3	1	theme	Protein	352:358	arg1	engineering					360:370	Protein engineering	352:370	Protein engineering	352:370	Protein engineering is useful to remedy this, but it usually requires prior structural knowledge of the target enzyme, and/or relies on extensive, time-consuming screening and analysis.					
33914359	5	2	theme	single-mutant	680:692	arg1	candidates					694:703	6-12 single-mutant candidates	675:703	6-12 single-mutant candidates	675:703	The method pinpoints 6-12 single-mutant candidates to improve transglycosylation yields.					
33914359	6	3	dep	other	802:806	arg1	than					808:811	than	808:811	than	808:811	Requiring very little prior knowledge of the target enzyme other than its sequence, the method is generic and procures catalysts for the formation of glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action.					
33914359	6	4	theme	glycosidic	893:902	arg1	bonds					904:908	glycosidic bonds	893:908	glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action	893:987	Requiring very little prior knowledge of the target enzyme other than its sequence, the method is generic and procures catalysts for the formation of glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action.					
33914359	4	5	theme	protein	622:628	arg1	sequences					630:638	protein sequences	622:638	protein sequences	622:638	Here, a straightforward strategy that involves rational rapid in silico analysis of protein sequences is described.					
33914359	4	6	dep	in	600:601	arg1	silico					603:608	silico	603:608	silico	603:608	Here, a straightforward strategy that involves rational rapid in silico analysis of protein sequences is described.					
33914359	2	7	theme	hydrolytic	314:323	arg1	activity					325:332	the innate hydrolytic activity	303:332	the innate hydrolytic activity of these enzymes	303:349	Enzyme-catalysed glycosylation using glycoside hydrolases is feasible but is hampered by the innate hydrolytic activity of these enzymes.					
33914359	2	8	theme	innate	307:312	arg1	activity					325:332	the innate hydrolytic activity	303:332	the innate hydrolytic activity of these enzymes	303:349	Enzyme-catalysed glycosylation using glycoside hydrolases is feasible but is hampered by the innate hydrolytic activity of these enzymes.					
33914359	6	9	theme	prior	765:769	arg1	knowledge					771:779	very little prior knowledge	753:779	very little prior knowledge of the target enzyme other than its sequence	753:824	Requiring very little prior knowledge of the target enzyme other than its sequence, the method is generic and procures catalysts for the formation of glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action.					
33914359	1	10	theme	synthetic	177:185	arg1	oligosaccharides					196:211	synthetic, defined oligosaccharides	177:211	oligosaccharides	196:211	Glycobiology is dogged by the relative scarcity of synthetic, defined oligosaccharides.					
33914359	0	11	theme	Transglycosylases	107:123	arg1	Generation					93:102	Efficient Generation	83:102	Efficient Generation of Transglycosylases	83:123	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.					
33914359	6	12	theme	little	758:763	arg1	knowledge					771:779	very little prior knowledge	753:779	very little prior knowledge of the target enzyme other than its sequence	753:824	Requiring very little prior knowledge of the target enzyme other than its sequence, the method is generic and procures catalysts for the formation of glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action.					
33914359	0	13	theme	Enzyme	9:14	arg1	Design					16:21	Rational Enzyme Design	0:21	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.	0:124	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.					
33914359	1	14	theme	defined	188:194	arg1	oligosaccharides					196:211	synthetic, defined oligosaccharides	177:211	oligosaccharides	196:211	Glycobiology is dogged by the relative scarcity of synthetic, defined oligosaccharides.					
33914359	0	15	theme	Rational	0:7	arg1	Design					16:21	Rational Enzyme Design	0:21	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.	0:124	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.					
33914359	4	16	theme	rational	585:592	arg1	analysis					610:617	rational rapid in silico analysis	585:617	rational rapid in silico analysis of protein sequences	585:638	Here, a straightforward strategy that involves rational rapid in silico analysis of protein sequences is described.					
33914359	2	17	theme	enzymes	343:349	arg1	activity					325:332	the innate hydrolytic activity	303:332	the innate hydrolytic activity of these enzymes	303:349	Enzyme-catalysed glycosylation using glycoside hydrolases is feasible but is hampered by the innate hydrolytic activity of these enzymes.					
33914359	3	18	theme	target	456:461	arg1	enzyme					463:468	the target enzyme	452:468	the target enzyme	452:468	Protein engineering is useful to remedy this, but it usually requires prior structural knowledge of the target enzyme, and/or relies on extensive, time-consuming screening and analysis.					
33914359	4	19	theme	in	600:601	arg1	analysis					610:617	rational rapid in silico analysis	585:617	rational rapid in silico analysis of protein sequences	585:638	Here, a straightforward strategy that involves rational rapid in silico analysis of protein sequences is described.					
33914359	6	20	theme	various	920:926	arg1	d/l-					928:931	various d/l-	920:931	various d/l-	920:931	Requiring very little prior knowledge of the target enzyme other than its sequence, the method is generic and procures catalysts for the formation of glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action.					
33914359	3	21	theme	extensive	488:496	arg1	screening					514:522	extensive, time-consuming screening	488:522	extensive, time-consuming screening	488:522	Protein engineering is useful to remedy this, but it usually requires prior structural knowledge of the target enzyme, and/or relies on extensive, time-consuming screening and analysis.					
33914359	4	22	theme	rapid	594:598	arg1	analysis					610:617	rational rapid in silico analysis	585:617	rational rapid in silico analysis of protein sequences	585:638	Here, a straightforward strategy that involves rational rapid in silico analysis of protein sequences is described.					
33914359	1	23	theme	oligosaccharides	196:211	arg1	scarcity					165:172	the relative scarcity	152:172	the relative scarcity of synthetic, defined oligosaccharides	152:211	Glycobiology is dogged by the relative scarcity of synthetic, defined oligosaccharides.					
33914359	0	24	theme	Structural	31:40	arg1	Knowledge					42:50	Structural Knowledge	31:50	Structural Knowledge	31:50	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.					
33914359	4	25	theme	straightforward	546:560	arg1	strategy					562:569	a straightforward strategy	544:569	a straightforward strategy that involves rational rapid in silico analysis of protein sequences	544:638	Here, a straightforward strategy that involves rational rapid in silico analysis of protein sequences is described.					
33914359	6	26	theme	bonds	904:908	arg1	formation					880:888	the formation	876:888	the formation of glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action	876:987	Requiring very little prior knowledge of the target enzyme other than its sequence, the method is generic and procures catalysts for the formation of glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action.					
33914359	3	27	theme	prior	422:426	arg1	knowledge					439:447	prior structural knowledge	422:447	prior structural knowledge of the target enzyme	422:468	Protein engineering is useful to remedy this, but it usually requires prior structural knowledge of the target enzyme, and/or relies on extensive, time-consuming screening and analysis.					
33914359	5	28	theme	transglycosylation	716:733	arg1	yields					735:740	transglycosylation yields	716:740	transglycosylation yields	716:740	The method pinpoints 6-12 single-mutant candidates to improve transglycosylation yields.					
33914359	3	29	theme	enzyme	463:468	arg1	knowledge					439:447	prior structural knowledge	422:447	prior structural knowledge of the target enzyme	422:468	Protein engineering is useful to remedy this, but it usually requires prior structural knowledge of the target enzyme, and/or relies on extensive, time-consuming screening and analysis.					
33914359	3	30	theme	structural	428:437	arg1	knowledge					439:447	prior structural knowledge	422:447	prior structural knowledge of the target enzyme	422:468	Protein engineering is useful to remedy this, but it usually requires prior structural knowledge of the target enzyme, and/or relies on extensive, time-consuming screening and analysis.					
33914359	6	31	theme	endo	977:980	arg1	action					982:987	endo action	977:987	endo action	977:987	Requiring very little prior knowledge of the target enzyme other than its sequence, the method is generic and procures catalysts for the formation of glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action.					
33914359	7	32	theme	related	1078:1084	arg1	enzymes					1086:1092	distantly related enzymes	1068:1092	distantly related enzymes	1068:1092	Moreover, mutations validated in one enzyme can be transposed to others, even distantly related enzymes.					
33914359	7	32	theme	related	1078:1084	arg1	others					1055:1060	others	1055:1060	others	1055:1060	Moreover, mutations validated in one enzyme can be transposed to others, even distantly related enzymes.					
33914359	2	33	theme	glycoside	251:259	arg1	hydrolases					261:270	glycoside hydrolases	251:270	glycoside hydrolases	251:270	Enzyme-catalysed glycosylation using glycoside hydrolases is feasible but is hampered by the innate hydrolytic activity of these enzymes.					
33914359	2	34	theme	Enzyme-catalysed	214:229	arg1	glycosylation					231:243	Enzyme-catalysed glycosylation	214:243	Enzyme-catalysed glycosylation using glycoside hydrolases	214:270	Enzyme-catalysed glycosylation using glycoside hydrolases is feasible but is hampered by the innate hydrolytic activity of these enzymes.					
33914359	0	35	dep	Design	16:21	arg1	Approach					70:77	A Sequence-Based Approach	53:77	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.	0:124	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.					
33914359	6	36	theme	enzyme	795:800	arg1	knowledge					771:779	very little prior knowledge	753:779	very little prior knowledge of the target enzyme other than its sequence	753:824	Requiring very little prior knowledge of the target enzyme other than its sequence, the method is generic and procures catalysts for the formation of glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action.					
33914359	4	37	theme	sequences	630:638	arg1	analysis					610:617	rational rapid in silico analysis	585:617	rational rapid in silico analysis of protein sequences	585:638	Here, a straightforward strategy that involves rational rapid in silico analysis of protein sequences is described.					
33914359	6	38	theme	target	788:793	arg1	enzyme					795:800	the target enzyme	784:800	the target enzyme	784:800	Requiring very little prior knowledge of the target enzyme other than its sequence, the method is generic and procures catalysts for the formation of glycosidic bonds involving various d/l-, α/β-pyranosides or furanosides, and exo or endo action.					
33914359	0	39	theme	Sequence-Based	55:68	arg1	Approach					70:77	A Sequence-Based Approach	53:77	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.	0:124	Rational Enzyme Design without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Transglycosylases.					
33914359	3	40	dep	extensive	488:496	arg1	time-consuming					499:512	time-consuming	499:512	time-consuming	499:512	Protein engineering is useful to remedy this, but it usually requires prior structural knowledge of the target enzyme, and/or relies on extensive, time-consuming screening and analysis.					
33914359	1	41	theme	relative	156:163	arg1	scarcity					165:172	the relative scarcity	152:172	the relative scarcity of synthetic, defined oligosaccharides	152:211	Glycobiology is dogged by the relative scarcity of synthetic, defined oligosaccharides.					
34494876	10	0	theme	S	1649:1649	arg1	protein					1651:1657	SARS-CoV-2 S protein	1638:1657	SARS-CoV-2 S protein	1638:1657	Impairment of hACE2 N-glycosylation decreased cell-to-cell fusion mediated by SARS-CoV S protein but not that mediated by SARS-CoV-2 S protein.					
34494876	6	1	theme	porcine	870:876	arg1	ACE2					878:881	porcine ACE2	870:881	porcine ACE2 (pACE2)	870:889	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	6	1	theme	porcine	870:876	arg1	pACE2					884:888	pACE2	884:888	pACE2	884:888	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	4	2	theme	protein	557:563	arg1	folding					565:571	protein folding	557:571	protein folding	557:571	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	14	3	theme	pseudotyped	2296:2306	arg1	viruses					2308:2314	S pseudotyped viruses	2294:2314	S pseudotyped viruses	2294:2314	In addition, we found that the removal of N-glycans of ACE2 impaired its ability to support an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses.					
34494876	12	4	theme	virus-receptor	1942:1955	arg1	interaction					1957:1967	the virus-receptor interaction	1938:1967	the virus-receptor interaction	1938:1967	IMPORTANCE Understanding the role of glycosylation in the virus-receptor interaction is important for developing approaches that disrupt infection.					
34494876	13	5	gly	deglycosylation	2062:2076	arg1	S					2095:2095	S	2095:2095	S	2095:2095	In this study, we showed that deglycosylation of both ACE2 and S had a minimal effect on the spike-ACE2 interaction.					
34494876	13	5	gly	deglycosylation	2062:2076	arg1	ACE2					2086:2089	ACE2	2086:2089	ACE2	2086:2089	In this study, we showed that deglycosylation of both ACE2 and S had a minimal effect on the spike-ACE2 interaction.					
34494876	7	6	theme	surface	1044:1050	arg1	expression					1052:1061	the proper cell surface expression	1028:1061	the proper cell surface expression of ACE2	1028:1069	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.					
34494876	14	7	theme	SARS-CoV-2	2283:2292	arg1	transduction					2254:2265	an efficient transduction	2241:2265	an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses	2241:2314	In addition, we found that the removal of N-glycans of ACE2 impaired its ability to support an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses.					
34494876	11	8	theme	efficient	1724:1732	arg1	entry					1740:1744	an efficient viral entry	1721:1744	an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor	1721:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	7	9	theme	proper	1032:1037	arg1	expression					1052:1061	the proper cell surface expression	1028:1061	the proper cell surface expression of ACE2	1028:1069	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.					
34494876	14	10	theme	SARS-CoV	2270:2277	arg1	transduction					2254:2265	an efficient transduction	2241:2265	an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses	2241:2314	In addition, we found that the removal of N-glycans of ACE2 impaired its ability to support an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses.					
34494876	5	11	theme	N-glycosylation	666:680	arg1	contribution					650:661	the contribution	646:661	the contribution of N-glycosylation to ACE2 function	646:697	However, the contribution of N-glycosylation to ACE2 function is poorly understood.					
34494876	3	12	theme	N-linked	460:467	arg1	glycans					469:475	N-linked glycans	460:475	N-linked glycans	460:475	Previous work has demonstrated the presence of N-linked glycans in ACE2.					
34494876	9	13	theme	proteins	1384:1391	arg1	deglycosylation					1355:1369	deglycosylation	1355:1369	deglycosylation of ACE2 or S proteins	1355:1391	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	0	14	from	Expression	63:72	arg1	Analysis					0:7	Analysis	0:7	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.	0:135	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	16	15	theme	ACE2	2696:2699	arg1	recruitment					2701:2711	ACE2 recruitment	2696:2711	ACE2 recruitment to the cell membrane	2696:2732	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	11	16	theme	low	1819:1821	arg1	expression					1836:1845	low cell surface expression	1819:1845	low cell surface expression of the deglycosylated ACE2 receptor	1819:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	4	17	theme	surface	601:607	arg1	expression					609:618	cell surface expression	596:618	cell surface expression	596:618	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	11	18	theme	SARS-CoV/SARS-CoV-2	1749:1767	arg1	viruses					1783:1789	SARS-CoV/SARS-CoV-2 S pseudotyped viruses	1749:1789	SARS-CoV/SARS-CoV-2 S pseudotyped viruses	1749:1789	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	11	18	theme	SARS-CoV/SARS-CoV-2	1749:1767	arg1	result					1809:1814	the result	1805:1814	the result of low cell surface expression of the deglycosylated ACE2 receptor	1805:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	5	19	theme	ACE2	685:688	arg1	function					690:697	ACE2 function	685:697	ACE2 function	685:697	However, the contribution of N-glycosylation to ACE2 function is poorly understood.					
34494876	8	20	theme	endoplasmic	1182:1192	arg1	ER					1205:1206	ER	1205:1206	ER	1205:1206	Furthermore, nonglycosylable ACE2 was localized predominantly in the endoplasmic reticulum (ER) and not at the cell surface.					
34494876	8	20	theme	endoplasmic	1182:1192	arg1	reticulum					1194:1202	the endoplasmic reticulum	1178:1202	the endoplasmic reticulum (ER)	1178:1207	Furthermore, nonglycosylable ACE2 was localized predominantly in the endoplasmic reticulum (ER) and not at the cell surface.					
34494876	10	21	theme	hACE2	1530:1534	arg1	N-glycosylation					1536:1550	hACE2 N-glycosylation	1530:1550	hACE2 N-glycosylation	1530:1550	Impairment of hACE2 N-glycosylation decreased cell-to-cell fusion mediated by SARS-CoV S protein but not that mediated by SARS-CoV-2 S protein.					
34494876	0	22	theme	ACE2	131:134	arg1	Expression					63:72	Cell Surface Expression	50:72	Cell Surface Expression	50:72	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	0	22	theme	ACE2	131:134	arg1	Properties					97:106	Binding Properties	89:106	Binding Properties	89:106	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	0	22	theme	ACE2	131:134	arg1	Function					75:82	Function	75:82	Function	75:82	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	11	23	theme	receptor	1874:1881	arg1	expression					1836:1845	low cell surface expression	1819:1845	low cell surface expression of the deglycosylated ACE2 receptor	1819:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	16	24	theme	cell	2720:2723	arg1	membrane					2725:2732	the cell membrane	2716:2732	the cell membrane	2716:2732	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	14	25	theme	efficient	2244:2252	arg1	transduction					2254:2265	an efficient transduction	2241:2265	an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses	2241:2314	In addition, we found that the removal of N-glycans of ACE2 impaired its ability to support an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses.					
34494876	0	26	from	Function	75:82	arg1	Analysis					0:7	Analysis	0:7	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.	0:135	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	0	27	theme	Cell	50:53	arg1	Expression					63:72	Cell Surface Expression	50:72	Cell Surface Expression	50:72	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	4	28	gly	N-glycosylation	486:500	arg1	activities					535:544	many biological activities	519:544	many biological activities	519:544	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	28	gly	N-glycosylation	486:500	arg1	folding					565:571	protein folding	557:571	protein folding	557:571	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	28	gly	N-glycosylation	486:500	arg1	expression					609:618	cell surface expression	596:618	cell surface expression	596:618	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	28	gly	N-glycosylation	486:500	arg1	activity					582:589	protein activity	574:589	protein activity	574:589	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	1	29	theme	entry	254:258	arg1	receptor					260:267	the major cell entry receptor	239:267	the major cell entry receptor for SARS-CoV and SARS-CoV-2	239:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	29	theme	entry	254:258	arg1	glycoprotein					211:222	a type I transmembrane glycoprotein	188:222	a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2	188:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	13	30	theme	spike-ACE2	2125:2134	arg1	interaction					2136:2146	the spike-ACE2 interaction	2121:2146	the spike-ACE2 interaction	2121:2146	In this study, we showed that deglycosylation of both ACE2 and S had a minimal effect on the spike-ACE2 interaction.					
34494876	1	31	theme	angiotensin	143:153	arg1	glycoprotein					211:222	a type I transmembrane glycoprotein	188:222	a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2	188:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	31	theme	angiotensin	143:153	arg1	enzyme					168:173	Human angiotensin I-converting enzyme 2	137:175	Human angiotensin I-converting enzyme 2 (hACE2)	137:183	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	31	theme	angiotensin	143:153	arg1	hACE2					178:182	hACE2	178:182	hACE2	178:182	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	0	32	from	Analysis	0:7	arg1	Expression					63:72	Cell Surface Expression	50:72	Cell Surface Expression	50:72	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	0	32	from	Analysis	0:7	arg1	Properties					97:106	Binding Properties	89:106	Binding Properties	89:106	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	0	32	from	Analysis	0:7	arg1	Function					75:82	Function	75:82	Function	75:82	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	11	33	theme	hACE2	1683:1687	arg1	N-glycosylation					1689:1703	hACE2 N-glycosylation	1683:1703	hACE2 N-glycosylation	1683:1703	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	1	34	gly	glycoprotein	211:222	arg1	receptor					260:267	the major cell entry receptor	239:267	the major cell entry receptor for SARS-CoV and SARS-CoV-2	239:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	34	gly	glycoprotein	211:222	arg1	glycoprotein					211:222	a type I transmembrane glycoprotein	188:222	a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2	188:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	34	gly	glycoprotein	211:222	arg1	enzyme					168:173	Human angiotensin I-converting enzyme 2	137:175	Human angiotensin I-converting enzyme 2 (hACE2)	137:183	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	11	35	gly	N-glycosylation	1689:1703	arg1	viruses					1783:1789	SARS-CoV/SARS-CoV-2 S pseudotyped viruses	1749:1789	SARS-CoV/SARS-CoV-2 S pseudotyped viruses	1749:1789	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	11	35	gly	N-glycosylation	1689:1703	arg1	result					1809:1814	the result	1805:1814	the result of low cell surface expression of the deglycosylated ACE2 receptor	1805:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	10	36	theme	SARS-CoV	1594:1601	arg1	protein					1605:1611	SARS-CoV S protein	1594:1611	SARS-CoV S protein but not that mediated by SARS-CoV-2 S protein	1594:1657	Impairment of hACE2 N-glycosylation decreased cell-to-cell fusion mediated by SARS-CoV S protein but not that mediated by SARS-CoV-2 S protein.					
34494876	15	37	theme	reduced	2416:2422	arg1	expression					2424:2433	a reduced expression	2414:2433	a reduced expression of the viral receptor on the cell surface	2414:2475	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34494876	9	38	theme	SARS	1474:1477	arg1	coronaviruses					1479:1491	SARS coronaviruses	1474:1491	SARS coronaviruses	1474:1491	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	6	39	with	species	807:813	arg1	susceptibility					830:843	different susceptibility	820:843	different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2	820:899	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	2	40	theme	membrane	396:403	arg1	fusion					405:410	subsequent virus-host cell membrane fusion	369:410	subsequent virus-host cell membrane fusion	369:410	The viral spike (S) protein is required for the attachment to ACE2 and subsequent virus-host cell membrane fusion.					
34494876	1	41	theme	major	243:247	arg1	receptor					260:267	the major cell entry receptor	239:267	the major cell entry receptor for SARS-CoV and SARS-CoV-2	239:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	41	theme	major	243:247	arg1	glycoprotein					211:222	a type I transmembrane glycoprotein	188:222	a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2	188:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	12	42	from	role	1913:1916	arg1	interaction					1957:1967	the virus-receptor interaction	1938:1967	the virus-receptor interaction	1938:1967	IMPORTANCE Understanding the role of glycosylation in the virus-receptor interaction is important for developing approaches that disrupt infection.					
34494876	2	43	theme	virus-host	380:389	arg1	fusion					405:410	subsequent virus-host cell membrane fusion	369:410	subsequent virus-host cell membrane fusion	369:410	The viral spike (S) protein is required for the attachment to ACE2 and subsequent virus-host cell membrane fusion.					
34494876	14	44	theme	S	2294:2294	arg1	viruses					2308:2314	S pseudotyped viruses	2294:2314	S pseudotyped viruses	2294:2314	In addition, we found that the removal of N-glycans of ACE2 impaired its ability to support an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses.					
34494876	3	45	theme	Previous	413:420	arg1	work					422:425	Previous work	413:425	Previous work	413:425	Previous work has demonstrated the presence of N-linked glycans in ACE2.					
34494876	9	46	theme	SARS-CoV	1277:1284	arg1	protein					1302:1308	SARS-CoV or SARS-CoV-2 S protein	1277:1308	protein	1302:1308	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	9	47	theme	ACE2	1501:1504	arg1	receptor					1506:1513	the ACE2 receptor	1497:1513	the ACE2 receptor	1497:1513	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	16	48	theme	coronavirus-related	2642:2660	arg1	illnesses					2662:2670	other coronavirus-related illnesses	2636:2670	other coronavirus-related illnesses	2636:2670	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	4	49	theme	many	519:522	arg1	activities					535:544	many biological activities	519:544	many biological activities	519:544	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	49	theme	many	519:522	arg1	folding					565:571	protein folding	557:571	protein folding	557:571	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	49	theme	many	519:522	arg1	expression					609:618	cell surface expression	596:618	cell surface expression	596:618	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	49	theme	many	519:522	arg1	activity					582:589	protein activity	574:589	protein activity	574:589	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	11	50	theme	expression	1836:1845	arg1	viruses					1783:1789	SARS-CoV/SARS-CoV-2 S pseudotyped viruses	1749:1789	SARS-CoV/SARS-CoV-2 S pseudotyped viruses	1749:1789	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	11	50	theme	expression	1836:1845	arg1	result					1809:1814	the result	1805:1814	the result of low cell surface expression of the deglycosylated ACE2 receptor	1805:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	7	51	theme	carboxiprotease	1087:1101	arg1	activity					1103:1110	its carboxiprotease activity	1083:1110	its carboxiprotease activity	1083:1110	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.					
34494876	9	52	theme	SARS-CoV-2	1289:1298	arg1	protein					1302:1308	SARS-CoV or SARS-CoV-2 S protein	1277:1308	protein	1302:1308	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	15	53	from	role	2343:2346	arg1	infection					2387:2395	reducing infection	2378:2395	reducing infection	2378:2395	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34494876	6	54	from	role	743:746	arg1	localization					787:798	localization	787:798	localization	787:798	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	6	54	from	role	743:746	arg1	activity					774:781	activity	774:781	activity	774:781	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	10	55	theme	SARS-CoV-2	1638:1647	arg1	protein					1651:1657	SARS-CoV-2 S protein	1638:1657	SARS-CoV-2 S protein	1638:1657	Impairment of hACE2 N-glycosylation decreased cell-to-cell fusion mediated by SARS-CoV S protein but not that mediated by SARS-CoV-2 S protein.					
34494876	0	56	theme	Glycosylation	33:45	arg1	Role					16:19	the Role	12:19	the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2	12:134	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	9	57	theme	protein	1302:1308	arg1	binding					1266:1272	binding	1266:1272	binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2	1266:1333	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	11	58	gly	deglycosylated	1854:1867	arg1	receptor					1874:1881	the deglycosylated ACE2 receptor	1850:1881	the deglycosylated ACE2 receptor	1850:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	16	59	theme	ACE2	2549:2552	arg1	function					2537:2544	function	2537:2544	function	2537:2544	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	16	59	theme	ACE2	2549:2552	arg1	structure					2523:2531	glycan structure	2516:2531	glycan structure	2516:2531	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	11	60	theme	pseudotyped	1771:1781	arg1	viruses					1783:1789	SARS-CoV/SARS-CoV-2 S pseudotyped viruses	1749:1789	SARS-CoV/SARS-CoV-2 S pseudotyped viruses	1749:1789	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	11	60	theme	pseudotyped	1771:1781	arg1	result					1809:1814	the result	1805:1814	the result of low cell surface expression of the deglycosylated ACE2 receptor	1805:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	1	61	theme	type	190:193	arg1	receptor					260:267	the major cell entry receptor	239:267	the major cell entry receptor for SARS-CoV and SARS-CoV-2	239:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	61	theme	type	190:193	arg1	glycoprotein					211:222	a type I transmembrane glycoprotein	188:222	a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2	188:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	61	theme	type	190:193	arg1	enzyme					168:173	Human angiotensin I-converting enzyme 2	137:175	Human angiotensin I-converting enzyme 2 (hACE2)	137:183	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	3	62	attach	presence	448:455	arg1	ACE2					480:483	ACE2	480:483	ACE2	480:483	Previous work has demonstrated the presence of N-linked glycans in ACE2.					
34494876	3	62	attach	presence	448:455	arg2	glycans					469:475	N-linked glycans	460:475	N-linked glycans	460:475	Previous work has demonstrated the presence of N-linked glycans in ACE2.					
34494876	3	63	theme	glycans	469:475	arg1	presence					448:455	the presence	444:455	the presence of N-linked glycans in ACE2	444:483	Previous work has demonstrated the presence of N-linked glycans in ACE2.					
34494876	9	64	theme	human	1324:1328	arg1	ACE2					1330:1333	porcine or human ACE2	1313:1333	ACE2	1330:1333	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	4	65	theme	protein	574:580	arg1	activity					582:589	protein activity	574:589	protein activity	574:589	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	7	66	theme	ACE2	1066:1069	arg1	expression					1052:1061	the proper cell surface expression	1028:1061	the proper cell surface expression of ACE2	1028:1069	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.					
34494876	2	67	theme	S	315:315	arg1	protein					318:324	The viral spike (S) protein	298:324	The viral spike (S) protein	298:324	The viral spike (S) protein is required for the attachment to ACE2 and subsequent virus-host cell membrane fusion.					
34494876	13	68	theme	ACE2	2086:2089	arg1	deglycosylation					2062:2076	deglycosylation	2062:2076	deglycosylation of both ACE2 and S	2062:2095	In this study, we showed that deglycosylation of both ACE2 and S had a minimal effect on the spike-ACE2 interaction.					
34494876	16	69	theme	antiviral	2590:2598	arg1	therapies					2600:2608	future antiviral therapies	2583:2608	future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane	2583:2732	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	13	70	theme	S	2095:2095	arg1	deglycosylation					2062:2076	deglycosylation	2062:2076	deglycosylation of both ACE2 and S	2062:2095	In this study, we showed that deglycosylation of both ACE2 and S had a minimal effect on the spike-ACE2 interaction.					
34494876	12	71	theme	glycosylation	1921:1933	arg1	role					1913:1916	the role	1909:1916	the role of glycosylation in the virus-receptor interaction	1909:1967	IMPORTANCE Understanding the role of glycosylation in the virus-receptor interaction is important for developing approaches that disrupt infection.					
34494876	2	72	theme	spike	308:312	arg1	protein					318:324	The viral spike (S) protein	298:324	The viral spike (S) protein	298:324	The viral spike (S) protein is required for the attachment to ACE2 and subsequent virus-host cell membrane fusion.					
34494876	7	73	theme	cell	1039:1042	arg1	expression					1052:1061	the proper cell surface expression	1028:1061	the proper cell surface expression of ACE2	1028:1069	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.					
34494876	6	74	theme	N-glycosylation	751:765	arg1	role					743:746	the role	739:746	the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2	739:899	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	1	75	theme	I-converting	155:166	arg1	glycoprotein					211:222	a type I transmembrane glycoprotein	188:222	a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2	188:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	75	theme	I-converting	155:166	arg1	enzyme					168:173	Human angiotensin I-converting enzyme 2	137:175	Human angiotensin I-converting enzyme 2 (hACE2)	137:183	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	75	theme	I-converting	155:166	arg1	hACE2					178:182	hACE2	178:182	hACE2	178:182	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	6	76	theme	SARS-CoV-2	848:857	arg1	infection					859:867	SARS-CoV-2 infection	848:867	SARS-CoV-2 infection	848:867	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	9	77	theme	ACE2	1374:1377	arg1	deglycosylation					1355:1369	deglycosylation	1355:1369	deglycosylation of ACE2 or S proteins	1355:1391	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	8	78	theme	nonglycosylable	1126:1140	arg1	ACE2					1142:1145	nonglycosylable ACE2	1126:1145	nonglycosylable ACE2	1126:1145	Furthermore, nonglycosylable ACE2 was localized predominantly in the endoplasmic reticulum (ER) and not at the cell surface.					
34494876	15	79	gly	deglycosylation	2351:2365	arg1	ACE2					2370:2373	ACE2	2370:2373	ACE2	2370:2373	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34494876	4	80	theme	biomolecules	623:634	arg1	expression					609:618	cell surface expression	596:618	cell surface expression	596:618	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	80	theme	biomolecules	623:634	arg1	folding					565:571	protein folding	557:571	protein folding	557:571	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	80	theme	biomolecules	623:634	arg1	activity					582:589	protein activity	574:589	protein activity	574:589	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	11	81	theme	viral	1734:1738	arg1	entry					1740:1744	an efficient viral entry	1721:1744	an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor	1721:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	3	82	from	presence	448:455	arg1	ACE2					480:483	ACE2	480:483	ACE2	480:483	Previous work has demonstrated the presence of N-linked glycans in ACE2.					
34494876	15	83	theme	viral	2442:2446	arg1	receptor					2448:2455	the viral receptor	2438:2455	the viral receptor	2438:2455	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34494876	9	84	theme	S	1382:1382	arg1	proteins					1384:1391	S proteins	1382:1391	S proteins	1382:1391	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	16	85	theme	glycan	2516:2521	arg1	structure					2523:2531	glycan structure	2516:2531	glycan structure	2516:2531	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	3	86	link	N-linked	460:467	arg1	glycans					469:475	N-linked glycans	460:475	N-linked glycans	460:475	Previous work has demonstrated the presence of N-linked glycans in ACE2.					
34494876	6	87	dep	activity	774:781	arg1	the					770:772	the	770:772	the	770:772	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	10	88	theme	cell-to-cell	1562:1573	arg1	fusion					1575:1580	cell-to-cell fusion	1562:1580	cell-to-cell fusion mediated by SARS-CoV S protein but not that mediated by SARS-CoV-2 S protein	1562:1657	Impairment of hACE2 N-glycosylation decreased cell-to-cell fusion mediated by SARS-CoV S protein but not that mediated by SARS-CoV-2 S protein.					
34494876	0	89	theme	Receptor	122:129	arg1	ACE2					131:134	SARS-CoV-2 Receptor ACE2	111:134	SARS-CoV-2 Receptor ACE2	111:134	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	4	90	theme	cell	596:599	arg1	expression					609:618	cell surface expression	596:618	cell surface expression	596:618	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	10	91	theme	N-glycosylation	1536:1550	arg1	Impairment					1516:1525	Impairment	1516:1525	Impairment of hACE2 N-glycosylation	1516:1550	Impairment of hACE2 N-glycosylation decreased cell-to-cell fusion mediated by SARS-CoV S protein but not that mediated by SARS-CoV-2 S protein.					
34494876	11	92	theme	S	1769:1769	arg1	viruses					1783:1789	SARS-CoV/SARS-CoV-2 S pseudotyped viruses	1749:1789	SARS-CoV/SARS-CoV-2 S pseudotyped viruses	1749:1789	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	11	92	theme	S	1769:1769	arg1	result					1809:1814	the result	1805:1814	the result of low cell surface expression of the deglycosylated ACE2 receptor	1805:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	11	93	theme	ACE2	1869:1872	arg1	receptor					1874:1881	the deglycosylated ACE2 receptor	1850:1881	the deglycosylated ACE2 receptor	1850:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	6	94	gly	N-glycosylation	751:765	arg1	species					807:813	two species	803:813	two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2	803:899	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	16	95	theme	recruitment	2701:2711	arg1	inhibition					2682:2691	inhibition	2682:2691	inhibition of ACE2 recruitment to the cell membrane	2682:2732	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	8	96	theme	cell	1224:1227	arg1	surface					1229:1235	the cell surface	1220:1235	the cell surface	1220:1235	Furthermore, nonglycosylable ACE2 was localized predominantly in the endoplasmic reticulum (ER) and not at the cell surface.					
34494876	15	97	theme	receptor	2448:2455	arg1	expression					2424:2433	a reduced expression	2414:2433	a reduced expression of the viral receptor on the cell surface	2414:2475	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34494876	15	98	from	expression	2424:2433	arg1	surface					2469:2475	the cell surface	2460:2475	the cell surface	2460:2475	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34494876	0	99	theme	Surface	55:61	arg1	Expression					63:72	Cell Surface Expression	50:72	Cell Surface Expression	50:72	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	15	100	theme	reducing	2378:2385	arg1	infection					2387:2395	reducing infection	2378:2395	reducing infection	2378:2395	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34494876	0	101	from	Role	16:19	arg1	Expression					63:72	Cell Surface Expression	50:72	Cell Surface Expression	50:72	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	0	101	from	Role	16:19	arg1	Properties					97:106	Binding Properties	89:106	Binding Properties	89:106	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	0	101	from	Role	16:19	arg1	Function					75:82	Function	75:82	Function	75:82	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	9	102	theme	porcine	1313:1319	arg1	ACE2					1330:1333	porcine or human ACE2	1313:1333	ACE2	1330:1333	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	15	103	theme	deglycosylation	2351:2365	arg1	role					2343:2346	the role	2339:2346	the role of deglycosylation of ACE2 on reducing infection	2339:2395	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34494876	15	103	theme	deglycosylation	2351:2365	arg1	due					2407:2409	due	2407:2409	due	2407:2409	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34494876	15	104	theme	ACE2	2370:2373	arg1	deglycosylation					2351:2365	deglycosylation	2351:2365	deglycosylation of ACE2	2351:2373	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34494876	1	105	theme	Human	137:141	arg1	glycoprotein					211:222	a type I transmembrane glycoprotein	188:222	a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2	188:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	105	theme	Human	137:141	arg1	enzyme					168:173	Human angiotensin I-converting enzyme 2	137:175	Human angiotensin I-converting enzyme 2 (hACE2)	137:183	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	105	theme	Human	137:141	arg1	hACE2					178:182	hACE2	178:182	hACE2	178:182	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	14	106	dep	SARS-CoV	2270:2277	arg1	viruses					2308:2314	S pseudotyped viruses	2294:2314	S pseudotyped viruses	2294:2314	In addition, we found that the removal of N-glycans of ACE2 impaired its ability to support an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses.					
34494876	7	107	gly	N-glycosylation	996:1010	arg1	cell					1039:1042	the proper cell surface expression	1028:1061	the proper cell surface expression of ACE2	1028:1069	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.					
34494876	7	107	gly	N-glycosylation	996:1010	arg1	carboxiprotease					1087:1101	its carboxiprotease activity	1083:1110	its carboxiprotease activity	1083:1110	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.					
34494876	7	107	gly	N-glycosylation	996:1010	arg1	surface					1044:1050	the proper cell surface expression	1028:1061	the proper cell surface expression of ACE2	1028:1069	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.					
34494876	7	107	gly	N-glycosylation	996:1010	arg1	ACE2					1066:1069	ACE2	1066:1069	ACE2	1066:1069	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.					
34494876	7	108	theme	N-glycosylation	921:935	arg1	elimination					906:916	The elimination	902:916	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis,	902:982	The elimination of N-glycosylation by tunicamycin (TM) treatment, or mutagenesis, showed that N-glycosylation is critical for the proper cell surface expression of ACE2 but not for its carboxiprotease activity.					
34494876	13	109	contain	had	2097:2099	arg1	deglycosylation					2062:2076	deglycosylation	2062:2076	deglycosylation of both ACE2 and S	2062:2095	In this study, we showed that deglycosylation of both ACE2 and S had a minimal effect on the spike-ACE2 interaction.					
34494876	13	109	contain	had	2097:2099	arg2	effect					2111:2116	a minimal effect	2101:2116	a minimal effect	2101:2116	In this study, we showed that deglycosylation of both ACE2 and S had a minimal effect on the spike-ACE2 interaction.					
34494876	14	110	theme	ACE2	2204:2207	arg1	N-glycans					2191:2199	N-glycans	2191:2199	N-glycans of ACE2	2191:2207	In addition, we found that the removal of N-glycans of ACE2 impaired its ability to support an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses.					
34494876	0	111	theme	Binding	89:95	arg1	Properties					97:106	Binding Properties	89:106	Binding Properties	89:106	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	13	112	theme	minimal	2103:2109	arg1	effect					2111:2116	a minimal effect	2101:2116	a minimal effect	2101:2116	In this study, we showed that deglycosylation of both ACE2 and S had a minimal effect on the spike-ACE2 interaction.					
34494876	14	113	theme	N-glycans	2191:2199	arg1	removal					2180:2186	the removal	2176:2186	the removal of N-glycans of ACE2	2176:2207	In addition, we found that the removal of N-glycans of ACE2 impaired its ability to support an efficient transduction of SARS-CoV and SARS-CoV-2 S pseudotyped viruses.					
34494876	11	114	theme	surface	1828:1834	arg1	expression					1836:1845	low cell surface expression	1819:1845	low cell surface expression of the deglycosylated ACE2 receptor	1819:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	16	115	theme	other	2636:2640	arg1	illnesses					2662:2670	other coronavirus-related illnesses	2636:2670	other coronavirus-related illnesses	2636:2670	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	2	116	theme	cell	391:394	arg1	fusion					405:410	subsequent virus-host cell membrane fusion	369:410	subsequent virus-host cell membrane fusion	369:410	The viral spike (S) protein is required for the attachment to ACE2 and subsequent virus-host cell membrane fusion.					
34494876	9	117	gly	deglycosylation	1355:1369	arg1	ACE2					1374:1377	ACE2	1374:1377	ACE2	1374:1377	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	9	117	gly	deglycosylation	1355:1369	arg1	proteins					1384:1391	S proteins	1382:1391	S proteins	1382:1391	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	0	118	theme	Role	16:19	arg1	Analysis					0:7	Analysis	0:7	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.	0:135	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	1	119	theme	cell	249:252	arg1	receptor					260:267	the major cell entry receptor	239:267	the major cell entry receptor for SARS-CoV and SARS-CoV-2	239:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	119	theme	cell	249:252	arg1	glycoprotein					211:222	a type I transmembrane glycoprotein	188:222	a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2	188:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	2	120	theme	subsequent	369:378	arg1	fusion					405:410	subsequent virus-host cell membrane fusion	369:410	subsequent virus-host cell membrane fusion	369:410	The viral spike (S) protein is required for the attachment to ACE2 and subsequent virus-host cell membrane fusion.					
34494876	4	121	theme	biological	524:533	arg1	activities					535:544	many biological activities	519:544	many biological activities	519:544	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	121	theme	biological	524:533	arg1	folding					565:571	protein folding	557:571	protein folding	557:571	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	121	theme	biological	524:533	arg1	expression					609:618	cell surface expression	596:618	cell surface expression	596:618	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	4	121	theme	biological	524:533	arg1	activity					582:589	protein activity	574:589	protein activity	574:589	N-glycosylation is implicated in many biological activities, including protein folding, protein activity, and cell surface expression of biomolecules.					
34494876	11	122	theme	deglycosylated	1854:1867	arg1	receptor					1874:1881	the deglycosylated ACE2 receptor	1850:1881	the deglycosylated ACE2 receptor	1850:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	0	123	theme	N-Linked	24:31	arg1	Glycosylation					33:45	N-Linked Glycosylation	24:45	N-Linked Glycosylation	24:45	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	16	124	dep	structure	2523:2531	arg1	the					2512:2514	the	2512:2514	the	2512:2514	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	6	125	theme	different	820:828	arg1	susceptibility					830:843	different susceptibility	820:843	different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2	820:899	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	11	126	theme	cell	1823:1826	arg1	expression					1836:1845	low cell surface expression	1819:1845	low cell surface expression of the deglycosylated ACE2 receptor	1819:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	1	127	theme	transmembrane	197:209	arg1	receptor					260:267	the major cell entry receptor	239:267	the major cell entry receptor for SARS-CoV and SARS-CoV-2	239:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	127	theme	transmembrane	197:209	arg1	glycoprotein					211:222	a type I transmembrane glycoprotein	188:222	a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2	188:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	127	theme	transmembrane	197:209	arg1	enzyme					168:173	Human angiotensin I-converting enzyme 2	137:175	Human angiotensin I-converting enzyme 2 (hACE2)	137:183	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	10	128	theme	S	1603:1603	arg1	protein					1605:1611	SARS-CoV S protein	1594:1611	SARS-CoV S protein but not that mediated by SARS-CoV-2 S protein	1594:1657	Impairment of hACE2 N-glycosylation decreased cell-to-cell fusion mediated by SARS-CoV S protein but not that mediated by SARS-CoV-2 S protein.					
34494876	9	129	theme	S	1300:1300	arg1	protein					1302:1308	SARS-CoV or SARS-CoV-2 S protein	1277:1308	protein	1302:1308	Our data also revealed that binding of SARS-CoV or SARS-CoV-2 S protein to porcine or human ACE2 was not affected by deglycosylation of ACE2 or S proteins, suggesting that N-glycosylation does not play a role in the interaction between SARS coronaviruses and the ACE2 receptor.					
34494876	6	130	theme	species	807:813	arg1	localization					787:798	localization	787:798	localization	787:798	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	6	130	theme	species	807:813	arg1	activity					774:781	activity	774:781	activity	774:781	Here, we examined the role of N-glycosylation in the activity and localization of two species with different susceptibility to SARS-CoV-2 infection, porcine ACE2 (pACE2) and hACE2.					
34494876	0	131	theme	SARS-CoV-2	111:120	arg1	ACE2					131:134	SARS-CoV-2 Receptor ACE2	111:134	SARS-CoV-2 Receptor ACE2	111:134	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	8	132	located	localized	1151:1159	arg1	ER					1205:1206	ER	1205:1206	ER	1205:1206	Furthermore, nonglycosylable ACE2 was localized predominantly in the endoplasmic reticulum (ER) and not at the cell surface.					
34494876	8	132	located	localized	1151:1159	arg1	reticulum					1194:1202	the endoplasmic reticulum	1178:1202	the endoplasmic reticulum (ER)	1178:1207	Furthermore, nonglycosylable ACE2 was localized predominantly in the endoplasmic reticulum (ER) and not at the cell surface.					
34494876	8	132	located	localized	1151:1159	arg2	ACE2					1142:1145	nonglycosylable ACE2	1126:1145	nonglycosylable ACE2	1126:1145	Furthermore, nonglycosylable ACE2 was localized predominantly in the endoplasmic reticulum (ER) and not at the cell surface.					
34494876	2	133	theme	viral	302:306	arg1	protein					318:324	The viral spike (S) protein	298:324	The viral spike (S) protein	298:324	The viral spike (S) protein is required for the attachment to ACE2 and subsequent virus-host cell membrane fusion.					
34494876	12	134	gly	glycosylation	1921:1933	arg1	virus-receptor					1942:1955	the virus-receptor interaction	1938:1967	the virus-receptor interaction	1938:1967	IMPORTANCE Understanding the role of glycosylation in the virus-receptor interaction is important for developing approaches that disrupt infection.					
34494876	11	135	theme	viruses	1783:1789	arg1	entry					1740:1744	an efficient viral entry	1721:1744	an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor	1721:1881	Finally, we found that hACE2 N-glycosylation is required for an efficient viral entry of SARS-CoV/SARS-CoV-2 S pseudotyped viruses, which may be the result of low cell surface expression of the deglycosylated ACE2 receptor.					
34494876	1	136	theme	I	195:195	arg1	receptor					260:267	the major cell entry receptor	239:267	the major cell entry receptor for SARS-CoV and SARS-CoV-2	239:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	136	theme	I	195:195	arg1	glycoprotein					211:222	a type I transmembrane glycoprotein	188:222	a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2	188:295	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	1	136	theme	I	195:195	arg1	enzyme					168:173	Human angiotensin I-converting enzyme 2	137:175	Human angiotensin I-converting enzyme 2 (hACE2)	137:183	Human angiotensin I-converting enzyme 2 (hACE2) is a type I transmembrane glycoprotein that serves as the major cell entry receptor for SARS-CoV and SARS-CoV-2.					
34494876	16	137	theme	future	2583:2588	arg1	therapies					2600:2608	future antiviral therapies	2583:2608	future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane	2583:2732	These findings offer insight into the glycan structure and function of ACE2 and potentially suggest that future antiviral therapies against coronaviruses and other coronavirus-related illnesses involving inhibition of ACE2 recruitment to the cell membrane could be developed.					
34494876	0	138	from	Properties	97:106	arg1	Analysis					0:7	Analysis	0:7	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.	0:135	Analysis of the Role of N-Linked Glycosylation in Cell Surface Expression, Function, and Binding Properties of SARS-CoV-2 Receptor ACE2.					
34494876	15	139	theme	cell	2464:2467	arg1	surface					2469:2475	the cell surface	2460:2475	the cell surface	2460:2475	Our data suggest that the role of deglycosylation of ACE2 on reducing infection is likely due to a reduced expression of the viral receptor on the cell surface.					
34364614	0	0	theme	anionic	75:81	arg1	polysaccharide					83:96	anionic polysaccharide	75:96	anionic polysaccharide	75:96	Fabrication of highly flexible nanochitin film and its composite film with anionic polysaccharide.					
34364614	1	1	theme	film	155:158	arg1	fabrication					127:137	the fabrication	123:137	the fabrication of a nanochitin film	123:158	This study investigated the fabrication of a nanochitin film via the aggregation of scaled-down chitin nanofibers (SD-ChNFs).					
34364614	2	2	dep	cationization	445:457	arg1	acid					515:518	aqueous acetic acid	500:518	aqueous acetic acid	500:518	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	4	3	theme	anionic	897:903	arg1	polysaccharide					905:918	an anionic polysaccharide	894:918	an anionic polysaccharide	894:918	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	4	3	theme	anionic	897:903	arg1	ι-carrageenan					928:940	ι-carrageenan	928:940	ι-carrageenan	928:940	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	5	4	theme	scaling	1077:1083	arg1	down					1085:1088	the scaling down	1073:1088	the scaling down of the ChNFs	1073:1101	These enhanced mechanical properties and efficient compositing properties were attributed to the scaling down of the ChNFs.					
34364614	4	5	theme	parent	781:786	arg1	film					808:811	the parent self-assembled ChNF film	777:811	the parent self-assembled ChNF film	777:811	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	0	6	with	film	65:68	arg1	polysaccharide					83:96	anionic polysaccharide	75:96	anionic polysaccharide	75:96	Fabrication of highly flexible nanochitin film and its composite film with anionic polysaccharide.					
34364614	2	7	from	cationization	445:457	arg1	1.0 mol/L					490:498	1.0 mol/L	490:498	1.0 mol/L	490:498	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	4	8	theme	ChNF	803:806	arg1	film					808:811	the parent self-assembled ChNF film	777:811	the parent self-assembled ChNF film	777:811	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	2	9	from	repulsion	477:485	arg1	1.0 mol/L					490:498	1.0 mol/L	490:498	1.0 mol/L	490:498	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	4	10	theme	self-assembled	788:801	arg1	film					808:811	the parent self-assembled ChNF film	777:811	the parent self-assembled ChNF film	777:811	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	2	11	theme	liquid	308:313	arg1	gel					319:321	a chitin/ionic liquid ion gel	293:321	a chitin/ionic liquid ion gel using methanol	293:336	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	4	12	theme	compositing	864:874	arg1	SD-ChNFs					880:887	the SD-ChNFs	876:887	the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan	860:940	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	2	13	theme	chitin/ionic	295:306	arg1	gel					319:321	a chitin/ionic liquid ion gel	293:321	a chitin/ionic liquid ion gel using methanol	293:336	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	1	14	theme	scaled-down	183:193	arg1	nanofibers					202:211	scaled-down chitin nanofibers	183:211	scaled-down chitin nanofibers (SD-ChNFs)	183:222	This study investigated the fabrication of a nanochitin film via the aggregation of scaled-down chitin nanofibers (SD-ChNFs).					
34364614	1	14	theme	scaled-down	183:193	arg1	SD-ChNFs					214:221	SD-ChNFs	214:221	SD-ChNFs	214:221	This study investigated the fabrication of a nanochitin film via the aggregation of scaled-down chitin nanofibers (SD-ChNFs).					
34364614	0	15	theme	flexible	22:29	arg1	film					42:45	highly flexible nanochitin film	15:45	highly flexible nanochitin film	15:45	Fabrication of highly flexible nanochitin film and its composite film with anionic polysaccharide.					
34364614	1	16	theme	chitin	195:200	arg1	nanofibers					202:211	scaled-down chitin nanofibers	183:211	scaled-down chitin nanofibers (SD-ChNFs)	183:222	This study investigated the fabrication of a nanochitin film via the aggregation of scaled-down chitin nanofibers (SD-ChNFs).					
34364614	1	16	theme	chitin	195:200	arg1	SD-ChNFs					214:221	SD-ChNFs	214:221	SD-ChNFs	214:221	This study investigated the fabrication of a nanochitin film via the aggregation of scaled-down chitin nanofibers (SD-ChNFs).					
34364614	4	17	theme	mechanical	743:752	arg1	properties					754:763	superior mechanical properties	734:763	superior mechanical properties	734:763	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	2	18	theme	SD-ChNF	551:557	arg1	dispersion					559:568	a SD-ChNF dispersion	549:568	a SD-ChNF dispersion	549:568	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	3	19	theme	flexible	651:658	arg1	film					680:683	a highly flexible self-assembled ChNF film	642:683	a highly flexible self-assembled ChNF film that bent and twisted easily	642:712	Isolation of the SD-ChNFs via filtration of the dispersion resulted in a highly flexible self-assembled ChNF film that bent and twisted easily.					
34364614	5	20	theme	enhanced	986:993	arg1	properties					1006:1015	These enhanced mechanical properties	980:1015	These enhanced mechanical properties	980:1015	These enhanced mechanical properties and efficient compositing properties were attributed to the scaling down of the ChNFs.					
34364614	1	21	theme	nanofibers	202:211	arg1	aggregation					168:178	the aggregation	164:178	the aggregation of scaled-down chitin nanofibers (SD-ChNFs)	164:222	This study investigated the fabrication of a nanochitin film via the aggregation of scaled-down chitin nanofibers (SD-ChNFs).					
34364614	0	22	theme	film	42:45	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of highly flexible nanochitin film	0:45	Fabrication of highly flexible nanochitin film and its composite film with anionic polysaccharide.					
34364614	0	22	theme	film	42:45	arg1	film					65:68	its composite film	51:68	its composite film with anionic polysaccharide	51:96	Fabrication of highly flexible nanochitin film and its composite film with anionic polysaccharide.					
34364614	4	23	theme	ionic	959:963	arg1	cross-linking					965:977	multi-point ionic cross-linking	947:977	multi-point ionic cross-linking	947:977	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	3	24	theme	self-assembled	660:673	arg1	film					680:683	a highly flexible self-assembled ChNF film	642:683	a highly flexible self-assembled ChNF film that bent and twisted easily	642:712	Isolation of the SD-ChNFs via filtration of the dispersion resulted in a highly flexible self-assembled ChNF film that bent and twisted easily.					
34364614	2	25	theme	self-assembled	227:240	arg1	film					247:250	A self-assembled ChNF film	225:250	A self-assembled ChNF film	225:250	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	2	26	theme	electrostatic	463:475	arg1	repulsion					477:485	electrostatic repulsion	463:485	electrostatic repulsion in 1.0 mol/L	463:498	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	0	27	theme	nanochitin	31:40	arg1	film					42:45	highly flexible nanochitin film	15:45	highly flexible nanochitin film	15:45	Fabrication of highly flexible nanochitin film and its composite film with anionic polysaccharide.					
34364614	2	28	theme	aqueous	380:386	arg1	NaOH					388:391	aqueous NaOH	380:391	aqueous NaOH	380:391	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	0	29	theme	composite	55:63	arg1	film					65:68	its composite film	51:68	its composite film with anionic polysaccharide	51:96	Fabrication of highly flexible nanochitin film and its composite film with anionic polysaccharide.					
34364614	3	30	theme	SD-ChNFs	588:595	arg1	Isolation					571:579	Isolation	571:579	Isolation of the SD-ChNFs via filtration of the dispersion	571:628	Isolation of the SD-ChNFs via filtration of the dispersion resulted in a highly flexible self-assembled ChNF film that bent and twisted easily.					
34364614	4	31	theme	multi-point	947:957	arg1	cross-linking					965:977	multi-point ionic cross-linking	947:977	multi-point ionic cross-linking	947:977	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	3	32	theme	dispersion	619:628	arg1	filtration					601:610	filtration	601:610	filtration of the dispersion	601:628	Isolation of the SD-ChNFs via filtration of the dispersion resulted in a highly flexible self-assembled ChNF film that bent and twisted easily.					
34364614	5	33	theme	compositing	1031:1041	arg1	properties					1043:1052	efficient compositing properties	1021:1052	efficient compositing properties	1021:1052	These enhanced mechanical properties and efficient compositing properties were attributed to the scaling down of the ChNFs.					
34364614	0	34	with	Fabrication	0:10	arg1	polysaccharide					83:96	anionic polysaccharide	75:96	anionic polysaccharide	75:96	Fabrication of highly flexible nanochitin film and its composite film with anionic polysaccharide.					
34364614	4	35	theme	superior	734:741	arg1	properties					754:763	superior mechanical properties	734:763	superior mechanical properties	734:763	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	5	36	theme	efficient	1021:1029	arg1	properties					1043:1052	efficient compositing properties	1021:1052	efficient compositing properties	1021:1052	These enhanced mechanical properties and efficient compositing properties were attributed to the scaling down of the ChNFs.					
34364614	3	37	theme	ChNF	675:678	arg1	film					680:683	a highly flexible self-assembled ChNF film	642:683	a highly flexible self-assembled ChNF film that bent and twisted easily	642:712	Isolation of the SD-ChNFs via filtration of the dispersion resulted in a highly flexible self-assembled ChNF film that bent and twisted easily.					
34364614	5	38	theme	mechanical	995:1004	arg1	properties					1006:1015	These enhanced mechanical properties	980:1015	These enhanced mechanical properties	980:1015	These enhanced mechanical properties and efficient compositing properties were attributed to the scaling down of the ChNFs.					
34364614	2	39	theme	ion	315:317	arg1	gel					319:321	a chitin/ionic liquid ion gel	293:321	a chitin/ionic liquid ion gel using methanol	293:336	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	5	40	theme	ChNFs	1097:1101	arg1	down					1085:1088	the scaling down	1073:1088	the scaling down of the ChNFs	1073:1101	These enhanced mechanical properties and efficient compositing properties were attributed to the scaling down of the ChNFs.					
34364614	2	41	theme	acetic	508:513	arg1	acid					515:518	aqueous acetic acid	500:518	aqueous acetic acid	500:518	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	2	42	with	cationization	445:457	arg1	ultrasonication					525:539	ultrasonication	525:539	ultrasonication	525:539	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	4	43	with	SD-ChNFs	880:887	arg1	polysaccharide					905:918	an anionic polysaccharide	894:918	an anionic polysaccharide	894:918	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	4	43	with	SD-ChNFs	880:887	arg1	ι-carrageenan					928:940	ι-carrageenan	928:940	ι-carrageenan	928:940	The film exhibited superior mechanical properties compared to the parent self-assembled ChNF film, where the flexibility was further enhanced by the compositing the SD-ChNFs with an anionic polysaccharide, namely ι-carrageenan, via multi-point ionic cross-linking.					
34364614	2	44	theme	aqueous	500:506	arg1	acid					515:518	aqueous acetic acid	500:518	aqueous acetic acid	500:518	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	2	45	theme	ChNF	242:245	arg1	film					247:250	A self-assembled ChNF film	225:250	A self-assembled ChNF film	225:250	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	2	46	with	repulsion	477:485	arg1	ultrasonication					525:539	ultrasonication	525:539	ultrasonication	525:539	A self-assembled ChNF film, which was prepared by regeneration from a chitin/ionic liquid ion gel using methanol, followed by filtration, was treated with aqueous NaOH for deacetylation and subsequently disintegrated by cationization and electrostatic repulsion in 1.0 mol/L aqueous acetic acid with ultrasonication to give a SD-ChNF dispersion.					
34364614	1	47	theme	nanochitin	144:153	arg1	film					155:158	a nanochitin film	142:158	a nanochitin film	142:158	This study investigated the fabrication of a nanochitin film via the aggregation of scaled-down chitin nanofibers (SD-ChNFs).					
32390019	1	0	theme	l-sugar	193:199	arg1	strategy					213:220	l-sugar translation strategy	193:220	l-sugar translation strategy	193:220	The first synthesis of 3-deoxy-3-fluoro-l-fucose is presented, which employs a d- to l-sugar translation strategy, and involves an enzymatic oxidation of 3-deoxy-3-fluoro-l-fucitol.					
32390019	1	1	theme	3-deoxy-3-fluoro-l-fucose	131:155	arg1	synthesis					118:126	The first synthesis	108:126	The first synthesis of 3-deoxy-3-fluoro-l-fucose	108:155	The first synthesis of 3-deoxy-3-fluoro-l-fucose is presented, which employs a d- to l-sugar translation strategy, and involves an enzymatic oxidation of 3-deoxy-3-fluoro-l-fucitol.					
32390019	2	2	theme	fluorinated	396:406	arg1	trisaccharides					408:421	two fluorinated trisaccharides	392:421	two fluorinated trisaccharides	392:421	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32390019	2	3	theme	chemical	471:478	arg1	potential					440:448	its potential	436:448	its potential	436:448	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32390019	2	3	theme	chemical	471:478	arg1	probe					480:484	a novel versatile chemical probe	453:484	a novel versatile chemical probe in glycobiology	453:500	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32390019	1	4	theme	translation	201:211	arg1	strategy					213:220	l-sugar translation strategy	193:220	l-sugar translation strategy	193:220	The first synthesis of 3-deoxy-3-fluoro-l-fucose is presented, which employs a d- to l-sugar translation strategy, and involves an enzymatic oxidation of 3-deoxy-3-fluoro-l-fucitol.					
32390019	2	5	theme	versatile	461:469	arg1	potential					440:448	its potential	436:448	its potential	436:448	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32390019	2	5	theme	versatile	461:469	arg1	probe					480:484	a novel versatile chemical probe	453:484	a novel versatile chemical probe in glycobiology	453:500	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32390019	1	6	theme	3-deoxy-3-fluoro-l-fucitol	262:287	arg1	oxidation					249:257	an enzymatic oxidation	236:257	an enzymatic oxidation of 3-deoxy-3-fluoro-l-fucitol	236:287	The first synthesis of 3-deoxy-3-fluoro-l-fucose is presented, which employs a d- to l-sugar translation strategy, and involves an enzymatic oxidation of 3-deoxy-3-fluoro-l-fucitol.					
32390019	1	7	theme	first	112:116	arg1	synthesis					118:126	The first synthesis	108:126	The first synthesis of 3-deoxy-3-fluoro-l-fucose	108:155	The first synthesis of 3-deoxy-3-fluoro-l-fucose is presented, which employs a d- to l-sugar translation strategy, and involves an enzymatic oxidation of 3-deoxy-3-fluoro-l-fucitol.					
32390019	2	8	theme	α-1,3	357:361	arg1	fucosyltransferase					363:380	an α-1,3 fucosyltransferase	354:380	an α-1,3 fucosyltransferase	354:380	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32390019	0	9	theme	Chemoenzymatic	0:13	arg1	synthesis					15:23	Chemoenzymatic synthesis	0:23	Chemoenzymatic synthesis of 3-deoxy-3-fluoro-l-fucose	0:52	Chemoenzymatic synthesis of 3-deoxy-3-fluoro-l-fucose and its enzymatic incorporation into glycoconjugates.					
32390019	2	10	theme	Enzymatic	290:298	arg1	activation					300:309	Enzymatic activation	290:309	Enzymatic activation (FKP)	290:315	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32390019	2	10	theme	Enzymatic	290:298	arg1	FKP					312:314	FKP	312:314	FKP	312:314	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32390019	1	11	theme	enzymatic	239:247	arg1	oxidation					249:257	an enzymatic oxidation	236:257	an enzymatic oxidation of 3-deoxy-3-fluoro-l-fucitol	236:287	The first synthesis of 3-deoxy-3-fluoro-l-fucose is presented, which employs a d- to l-sugar translation strategy, and involves an enzymatic oxidation of 3-deoxy-3-fluoro-l-fucitol.					
32390019	0	12	theme	3-deoxy-3-fluoro-l-fucose	28:52	arg1	synthesis					15:23	Chemoenzymatic synthesis	0:23	Chemoenzymatic synthesis of 3-deoxy-3-fluoro-l-fucose	0:52	Chemoenzymatic synthesis of 3-deoxy-3-fluoro-l-fucose and its enzymatic incorporation into glycoconjugates.					
32390019	0	12	theme	3-deoxy-3-fluoro-l-fucose	28:52	arg1	incorporation					72:84	its enzymatic incorporation	58:84	its enzymatic incorporation into glycoconjugates	58:105	Chemoenzymatic synthesis of 3-deoxy-3-fluoro-l-fucose and its enzymatic incorporation into glycoconjugates.					
32390019	0	13	theme	enzymatic	62:70	arg1	incorporation					72:84	its enzymatic incorporation	58:84	its enzymatic incorporation into glycoconjugates	58:105	Chemoenzymatic synthesis of 3-deoxy-3-fluoro-l-fucose and its enzymatic incorporation into glycoconjugates.					
32390019	2	14	from	probe	480:484	arg1	glycobiology					489:500	glycobiology	489:500	glycobiology	489:500	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32390019	2	15	theme	novel	455:459	arg1	potential					440:448	its potential	436:448	its potential	436:448	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32390019	2	15	theme	novel	455:459	arg1	probe					480:484	a novel versatile chemical probe	453:484	a novel versatile chemical probe in glycobiology	453:500	Enzymatic activation (FKP) and glycosylation using an α-1,2 and an α-1,3 fucosyltransferase to obtain two fluorinated trisaccharides demonstrates its potential as a novel versatile chemical probe in glycobiology.					
32025672	0	0	theme	proteins	79:86	arg1	structure					30:38	structure	30:38	structure	30:38	Effects of irradiation on the structure and properties of glycosylated soybean proteins.					
32025672	0	0	theme	proteins	79:86	arg1	properties					44:53	properties	44:53	properties	44:53	Effects of irradiation on the structure and properties of glycosylated soybean proteins.					
32025672	2	1	from	changes	398:404	arg1	structure					413:421	the structure	409:421	the structure	409:421	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	1	2	theme	glycosylation	130:142	arg1	modification					144:155	the glycosylation modification	126:155	the glycosylation modification of soybean proteins under irradiation	126:193	At present, there are few reports on the glycosylation modification of soybean proteins under irradiation.					
32025672	2	3	used	used	254:257	arg2	maltose					241:247	maltose	241:247	maltose	241:247	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	2	3	used	used	254:257	arg2	isolate					229:235	a soybean protein isolate	211:235	a soybean protein isolate	211:235	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	2	3	used	used	254:257	arg2	materials					266:274	raw materials	262:274	raw materials	262:274	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	0	4	theme	soybean	71:77	arg1	proteins					79:86	glycosylated soybean proteins	58:86	glycosylated soybean proteins	58:86	Effects of irradiation on the structure and properties of glycosylated soybean proteins.					
32025672	9	5	theme	technical	1633:1641	arg1	support					1643:1649	theoretical and technical support	1617:1649	theoretical and technical support for expanding the application of the soy protein isolate in the food industry	1617:1727	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	2	6	theme	gamma-ray	324:332	arg1	treatment					334:342	gamma-ray treatment	324:342	gamma-ray treatment to improve the functional properties and evaluate the changes in the structure	324:421	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	7	7	theme	irradiation	1157:1167	arg1	technology					1169:1178	irradiation technology	1157:1178	irradiation technology	1157:1178	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	0	8	from	Effects	0:6	arg1	structure					30:38	structure	30:38	structure	30:38	Effects of irradiation on the structure and properties of glycosylated soybean proteins.					
32025672	0	8	from	Effects	0:6	arg1	properties					44:53	properties	44:53	properties	44:53	Effects of irradiation on the structure and properties of glycosylated soybean proteins.					
32025672	5	9	theme	±	966:966	arg1	%					972:972	41 ± 0.47%	963:972	41 ± 0.47%	963:972	The foaming property and foam stability increased by 62.5 ± 0.34% and 41 ± 0.47%, respectively.					
32025672	2	10	theme	raw	262:264	arg1	isolate					229:235	a soybean protein isolate	211:235	a soybean protein isolate	211:235	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	2	10	theme	raw	262:264	arg1	materials					266:274	raw materials	262:274	raw materials	262:274	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	2	10	theme	raw	262:264	arg1	maltose					241:247	maltose	241:247	maltose	241:247	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	8	11	theme	modification	1393:1404	arg1	method					1406:1411	the glycosylation modification method	1375:1411	the glycosylation modification method	1375:1411	This experiment combined irradiation technology with the glycosylation modification method.					
32025672	5	12	theme	foam	918:921	arg1	stability					923:931	foam stability	918:931	foam stability	918:931	The foaming property and foam stability increased by 62.5 ± 0.34% and 41 ± 0.47%, respectively.					
32025672	7	13	theme	high	1221:1224	arg1	efficiency					1226:1235	high efficiency	1221:1235	high efficiency	1221:1235	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	9	14	theme	functional	1560:1569	arg1	properties					1571:1580	the functional properties	1556:1580	the functional properties of the modified protein	1556:1604	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	3	15	theme	infrared	521:528	arg1	analysis					439:446	analysis	439:446	analysis of the graft degree	439:466	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	15	theme	infrared	521:528	arg1	spectrum					530:537	infrared spectrum	521:537	infrared spectrum	521:537	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	0	16	gly	glycosylated	58:69	arg1	proteins					79:86	glycosylated soybean proteins	58:86	glycosylated soybean proteins	58:86	Effects of irradiation on the structure and properties of glycosylated soybean proteins.					
32025672	3	17	theme	graft	455:459	arg1	degree					461:466	the graft degree	451:466	the graft degree	451:466	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	6	18	theme	compounds	1075:1083	arg1	capacity					1022:1029	water absorption capacity	1005:1029	water absorption capacity	1005:1029	Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly.					
32025672	6	18	theme	compounds	1075:1083	arg1	capacity					1050:1057	fat absorption capacity	1035:1057	fat absorption capacity	1035:1057	Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly.					
32025672	6	18	theme	compounds	1075:1083	arg1	Emulsification					989:1002	Emulsification	989:1002	Emulsification	989:1002	Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly.					
32025672	4	19	theme	products	819:826	arg1	solubility					796:805	the solubility	792:805	the solubility of modified products	792:826	When the irradiation dose was 7.5 kGy, the solubility of modified products increased by 23 ± 0.21% compared with that of the control group.					
32025672	3	20	theme	gel	500:502	arg1	analysis					439:446	analysis	439:446	analysis of the graft degree	439:466	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	20	theme	gel	500:502	arg1	electrophoresis					504:518	polyacrylamide gel electrophoresis	485:518	polyacrylamide gel electrophoresis	485:518	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	1	21	theme	proteins	168:175	arg1	modification					144:155	the glycosylation modification	126:155	the glycosylation modification of soybean proteins under irradiation	126:193	At present, there are few reports on the glycosylation modification of soybean proteins under irradiation.					
32025672	6	22	theme	absorption	1011:1020	arg1	capacity					1022:1029	water absorption capacity	1005:1029	water absorption capacity	1005:1029	Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly.					
32025672	4	23	theme	modified	810:817	arg1	products					819:826	modified products	810:826	modified products	810:826	When the irradiation dose was 7.5 kGy, the solubility of modified products increased by 23 ± 0.21% compared with that of the control group.					
32025672	9	24	theme	protein	1598:1604	arg1	properties					1571:1580	the functional properties	1556:1580	the functional properties of the modified protein	1556:1604	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	9	25	theme	Maillard	1463:1470	arg1	reaction					1472:1479	the Maillard reaction	1459:1479	the Maillard reaction between the soybean protein isolate and maltose	1459:1527	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	1	26	theme	soybean	160:166	arg1	proteins					168:175	soybean proteins	160:175	soybean proteins under irradiation	160:193	At present, there are few reports on the glycosylation modification of soybean proteins under irradiation.					
32025672	7	27	theme	other	1129:1133	arg1	methods					1148:1154	other modification methods	1129:1154	other modification methods	1129:1154	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	9	28	theme	protein	1501:1507	arg1	isolate					1509:1515	the soybean protein isolate	1489:1515	the soybean protein isolate	1489:1515	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	1	29	from	reports	115:121	arg1	modification					144:155	the glycosylation modification	126:155	the glycosylation modification of soybean proteins under irradiation	126:193	At present, there are few reports on the glycosylation modification of soybean proteins under irradiation.					
32025672	3	30	theme	fluorescence	540:551	arg1	analysis					439:446	analysis	439:446	analysis of the graft degree	439:466	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	30	theme	fluorescence	540:551	arg1	spectrum					553:560	fluorescence spectrum	540:560	fluorescence spectrum	540:560	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	7	31	theme	efficiency	1226:1235	arg1	advantages					1188:1197	the advantages	1184:1197	the advantages of short action time, high efficiency and low cost	1184:1248	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	2	32	theme	functional	359:368	arg1	properties					370:379	the functional properties	355:379	the functional properties	355:379	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	7	33	theme	modification	1135:1146	arg1	methods					1148:1154	other modification methods	1129:1154	other modification methods	1129:1154	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	3	34	theme	product	736:742	arg1	structure					710:718	the structure	706:718	the structure of the reaction product	706:742	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	5	35	theme	foaming	897:903	arg1	property					905:912	The foaming property	893:912	The foaming property	893:912	The foaming property and foam stability increased by 62.5 ± 0.34% and 41 ± 0.47%, respectively.					
32025672	9	36	theme	soybean	1493:1499	arg1	isolate					1509:1515	the soybean protein isolate	1489:1515	the soybean protein isolate	1489:1515	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	4	37	theme	control	878:884	arg1	group					886:890	the control group	874:890	the control group	874:890	When the irradiation dose was 7.5 kGy, the solubility of modified products increased by 23 ± 0.21% compared with that of the control group.					
32025672	9	38	theme	modified	1589:1596	arg1	protein					1598:1604	the modified protein	1585:1604	the modified protein	1585:1604	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	8	39	theme	irradiation	1347:1357	arg1	technology					1359:1368	irradiation technology	1347:1368	irradiation technology	1347:1368	This experiment combined irradiation technology with the glycosylation modification method.					
32025672	1	40	mod	modification	144:155	arg1	proteins					168:175	soybean proteins	160:175	soybean proteins under irradiation	160:193	At present, there are few reports on the glycosylation modification of soybean proteins under irradiation.					
32025672	1	40	mod	modification	144:155	arg3	glycosylation					130:142	the glycosylation modification	126:155	the glycosylation modification of soybean proteins under irradiation	126:193	At present, there are few reports on the glycosylation modification of soybean proteins under irradiation.					
32025672	0	41	theme	irradiation	11:21	arg1	Effects					0:6	Effects	0:6	Effects of irradiation on the structure and properties of glycosylated soybean proteins	0:86	Effects of irradiation on the structure and properties of glycosylated soybean proteins.					
32025672	4	42	theme	irradiation	762:772	arg1	dose					774:777	the irradiation dose	758:777	the irradiation dose	758:777	When the irradiation dose was 7.5 kGy, the solubility of modified products increased by 23 ± 0.21% compared with that of the control group.					
32025672	4	42	theme	irradiation	762:772	arg1	kGy					787:789	7.5 kGy	783:789	7.5 kGy	783:789	When the irradiation dose was 7.5 kGy, the solubility of modified products increased by 23 ± 0.21% compared with that of the control group.					
32025672	3	43	theme	Maillard	627:634	arg1	reaction					636:643	the Maillard reaction	623:643	the Maillard reaction between the soybean protein isolate	623:679	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	7	44	theme	action	1208:1213	arg1	time					1215:1218	short action time	1202:1218	short action time	1202:1218	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	7	45	theme	time	1215:1218	arg1	advantages					1188:1197	the advantages	1184:1197	the advantages of short action time, high efficiency and low cost	1184:1248	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	3	46	theme	browning	469:476	arg1	index					478:482	browning index	469:482	browning index	469:482	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	46	theme	browning	469:476	arg1	analysis					439:446	analysis	439:446	analysis of the graft degree	439:466	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	47	theme	reaction	727:734	arg1	product					736:742	the reaction product	723:742	the reaction product	723:742	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	9	48	theme	protein	1692:1698	arg1	isolate					1700:1706	the soy protein isolate	1684:1706	the soy protein isolate	1684:1706	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	6	49	theme	glycosylated	1062:1073	arg1	compounds					1075:1083	glycosylated compounds	1062:1083	glycosylated compounds	1062:1083	Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly.					
32025672	2	50	theme	soybean	213:219	arg1	isolate					229:235	a soybean protein isolate	211:235	a soybean protein isolate	211:235	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	2	50	theme	soybean	213:219	arg1	materials					266:274	raw materials	262:274	raw materials	262:274	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	2	50	theme	soybean	213:219	arg1	maltose					241:247	maltose	241:247	maltose	241:247	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	7	51	theme	cost	1245:1248	arg1	advantages					1188:1197	the advantages	1184:1197	the advantages of short action time, high efficiency and low cost	1184:1248	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	9	52	theme	soy	1688:1690	arg1	isolate					1700:1706	the soy protein isolate	1684:1706	the soy protein isolate	1684:1706	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	9	53	theme	isolate	1700:1706	arg1	application					1669:1679	the application	1665:1679	the application of the soy protein isolate	1665:1706	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	7	54	contain	had	1180:1182	arg2	advantages					1188:1197	the advantages	1184:1197	the advantages of short action time, high efficiency and low cost	1184:1248	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	7	54	contain	had	1180:1182	arg1	technology					1169:1178	irradiation technology	1157:1178	irradiation technology	1157:1178	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	0	55	dep	structure	30:38	arg1	the					26:28	the	26:28	the	26:28	Effects of irradiation on the structure and properties of glycosylated soybean proteins.					
32025672	9	56	theme	food	1715:1718	arg1	industry					1720:1727	the food industry	1711:1727	the food industry	1711:1727	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	3	57	theme	degree	461:466	arg1	spectrum					578:585	ultraviolet spectrum	566:585	ultraviolet spectrum	566:585	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	57	theme	degree	461:466	arg1	index					478:482	browning index	469:482	browning index	469:482	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	57	theme	degree	461:466	arg1	spectrum					530:537	infrared spectrum	521:537	infrared spectrum	521:537	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	57	theme	degree	461:466	arg1	spectrum					553:560	fluorescence spectrum	540:560	fluorescence spectrum	540:560	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	57	theme	degree	461:466	arg1	electrophoresis					504:518	polyacrylamide gel electrophoresis	485:518	polyacrylamide gel electrophoresis	485:518	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	57	theme	degree	461:466	arg1	analysis					439:446	analysis	439:446	analysis of the graft degree	439:466	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	58	theme	soybean	657:663	arg1	isolate					673:679	the soybean protein isolate	653:679	the soybean protein isolate	653:679	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	59	theme	ultraviolet	566:576	arg1	spectrum					578:585	ultraviolet spectrum	566:585	ultraviolet spectrum	566:585	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	59	theme	ultraviolet	566:576	arg1	analysis					439:446	analysis	439:446	analysis of the graft degree	439:466	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	7	60	theme	industrial	1277:1286	arg1	production					1288:1297	its industrial production	1273:1297	its industrial production	1273:1297	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	6	61	gly	glycosylated	1062:1073	arg1	compounds					1075:1083	glycosylated compounds	1062:1083	glycosylated compounds	1062:1083	Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly.					
32025672	8	62	theme	glycosylation	1379:1391	arg1	method					1406:1411	the glycosylation modification method	1375:1411	the glycosylation modification method	1375:1411	This experiment combined irradiation technology with the glycosylation modification method.					
32025672	3	63	theme	modified	594:601	arg1	product					603:609	the modified product	590:609	the modified product	590:609	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	2	64	gly	glycosylated	289:300	arg1	protein					310:316	a glycosylated soybean protein	287:316	a glycosylated soybean protein	287:316	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	3	65	theme	protein	665:671	arg1	isolate					673:679	the soybean protein isolate	653:679	the soybean protein isolate	653:679	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	66	theme	product	603:609	arg1	spectrum					578:585	ultraviolet spectrum	566:585	ultraviolet spectrum	566:585	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	66	theme	product	603:609	arg1	analysis					439:446	analysis	439:446	analysis of the graft degree	439:466	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	66	theme	product	603:609	arg1	spectrum					530:537	infrared spectrum	521:537	infrared spectrum	521:537	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	66	theme	product	603:609	arg1	spectrum					553:560	fluorescence spectrum	540:560	fluorescence spectrum	540:560	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	9	67	theme	theoretical	1617:1627	arg1	support					1643:1649	theoretical and technical support	1617:1649	theoretical and technical support for expanding the application of the soy protein isolate in the food industry	1617:1727	It was proved that irradiation could promote the Maillard reaction between the soybean protein isolate and maltose, and significantly improve the functional properties of the modified protein, providing theoretical and technical support for expanding the application of the soy protein isolate in the food industry.					
32025672	1	68	theme	few	111:113	arg1	reports					115:121	few reports	111:121	few reports on the glycosylation modification of soybean proteins under irradiation	111:193	At present, there are few reports on the glycosylation modification of soybean proteins under irradiation.					
32025672	7	69	theme	low	1241:1243	arg1	cost					1245:1248	low cost	1241:1248	low cost	1241:1248	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
32025672	5	70	theme	±	951:951	arg1	%					957:957	62.5 ± 0.34%	946:957	62.5 ± 0.34%	946:957	The foaming property and foam stability increased by 62.5 ± 0.34% and 41 ± 0.47%, respectively.					
32025672	2	71	theme	glycosylated	289:300	arg1	protein					310:316	a glycosylated soybean protein	287:316	a glycosylated soybean protein	287:316	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	2	72	theme	soybean	302:308	arg1	protein					310:316	a glycosylated soybean protein	287:316	a glycosylated soybean protein	287:316	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	0	73	theme	glycosylated	58:69	arg1	proteins					79:86	glycosylated soybean proteins	58:86	glycosylated soybean proteins	58:86	Effects of irradiation on the structure and properties of glycosylated soybean proteins.					
32025672	6	74	theme	fat	1035:1037	arg1	capacity					1050:1057	fat absorption capacity	1035:1057	fat absorption capacity	1035:1057	Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly.					
32025672	3	75	theme	analysis	439:446	arg1	results					428:434	The results	424:434	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product	424:609	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	6	76	theme	absorption	1039:1048	arg1	capacity					1050:1057	fat absorption capacity	1035:1057	fat absorption capacity	1035:1057	Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly.					
32025672	6	77	theme	water	1005:1009	arg1	capacity					1022:1029	water absorption capacity	1005:1029	water absorption capacity	1005:1029	Emulsification, water absorption capacity and fat absorption capacity of glycosylated compounds also increased significantly.					
32025672	2	78	theme	protein	221:227	arg1	isolate					229:235	a soybean protein isolate	211:235	a soybean protein isolate	211:235	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	2	78	theme	protein	221:227	arg1	materials					266:274	raw materials	262:274	raw materials	262:274	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	2	78	theme	protein	221:227	arg1	maltose					241:247	maltose	241:247	maltose	241:247	In this paper, a soybean protein isolate and maltose were used as raw materials to prepare a glycosylated soybean protein under gamma-ray treatment to improve the functional properties and evaluate the changes in the structure.					
32025672	3	79	theme	polyacrylamide	485:498	arg1	analysis					439:446	analysis	439:446	analysis of the graft degree	439:466	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	3	79	theme	polyacrylamide	485:498	arg1	electrophoresis					504:518	polyacrylamide gel electrophoresis	485:518	polyacrylamide gel electrophoresis	485:518	The results of analysis of the graft degree, browning index, polyacrylamide gel electrophoresis, infrared spectrum, fluorescence spectrum and ultraviolet spectrum of the modified product showed that the Maillard reaction between the soybean protein isolate and maltose occurred and the structure of the reaction product changed.					
32025672	7	80	theme	short	1202:1206	arg1	time					1215:1218	short action time	1202:1218	short action time	1202:1218	Compared with other modification methods, irradiation technology had the advantages of short action time, high efficiency and low cost, and more importantly, its industrial production was easy to implement.					
33675758	4	0	theme	glycosylated	481:492	arg1	IgG1					530:533	monoclonal human IgG1	513:533	glycosylated and deglycosylated monoclonal human IgG1 (designated as A33)	481:553	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	11	1	theme	best-fit	1557:1564	arg1	models					1566:1571	neutron best-fit models	1549:1571	neutron best-fit models	1549:1571	From these, 100 x-ray and neutron best-fit models were determined.					
33675758	13	2	theme	deglycosylated	1875:1888	arg1	IgG1					1890:1893	deglycosylated IgG1	1875:1893	deglycosylated IgG1	1875:1893	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	4	3	theme	deglycosylated	498:511	arg1	IgG1					530:533	monoclonal human IgG1	513:533	glycosylated and deglycosylated monoclonal human IgG1 (designated as A33)	481:553	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	8	4	theme	gyration	1092:1099	arg1	radius					1082:1087	the cross-sectional radius	1062:1087	the cross-sectional radius of gyration (RXS-1)	1062:1107	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	5	5	theme	IgG1	787:790	arg1	coefficients					764:775	the sedimentation coefficients s020,w	746:782	the sedimentation coefficients s020,w of IgG1	746:790	After deglycosylation using peptide:N-glycosidase F, analytical ultracentrifugation showed that IgG1 remained monomeric and the sedimentation coefficients s020,w of IgG1 decreased from 6.45 S by 0.16-0.27 S.					
33675758	8	6	theme	distance	1157:1164	arg1	M2					1171:1172	the commonly occurring distance peak M2	1134:1172	the commonly occurring distance peak M2 of the distance distribution curve P(r)	1134:1212	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	2	7	theme	Fab	251:253	arg1	regions					255:261	two Fab regions	247:261	two Fab regions connected to a Fc region through a 15-residue hinge peptide	247:321	IgG1 is composed of two Fab regions connected to a Fc region through a 15-residue hinge peptide.					
33675758	1	8	theme	prevalent	150:158	arg1	antibody					160:167	the most prevalent antibody	141:167	the most prevalent antibody in serum	141:176	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	1	8	theme	prevalent	150:158	arg1	class					132:136	The human immunoglobulin G (IgG) class	99:136	The human immunoglobulin G (IgG) class	99:136	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	12	9	theme	structural	1656:1665	arg1	conformations					1667:1679	structural conformations	1656:1679	structural conformations	1656:1679	For these, principal component analyses identified five groups of structural conformations that were different for glycosylated and deglycosylated IgG1.					
33675758	10	10	theme	deglycosylated	1489:1502	arg1	IgG1					1504:1507	deglycosylated IgG1	1489:1507	deglycosylated IgG1	1489:1507	To explain these changes, atomistic scattering modeling based on Monte Carlo simulations resulted in 123,284 and 119,191 trial structures for glycosylated and deglycosylated IgG1 respectively.					
33675758	1	11	from	antibody	160:167	arg1	serum					172:176	serum	172:176	serum	172:176	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	4	12	theme	forms	615:619	arg1	study					601:605	a comparative multidisciplinary structural study	558:605	a comparative multidisciplinary structural study of both forms	558:619	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	0	13	from	role	58:61	arg1	conformation					85:96	its conformation	81:96	its conformation	81:96	Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation.					
33675758	8	14	theme	distribution	1190:1201	arg1	r					1211:1211	r	1211:1211	r	1211:1211	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	8	14	theme	distribution	1190:1201	arg1	P					1209:1209	the distance distribution curve P	1177:1209	the distance distribution curve P(r)	1177:1212	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	7	15	theme	structural	965:974	arg1	parameters					976:985	the Guinier structural parameters	953:985	the Guinier structural parameters	953:985	X-ray and neutron scattering revealed changes in the Guinier structural parameters after deglycosylation.					
33675758	13	16	from	region	1750:1755	arg1	IgG1					1773:1776	glycosylated IgG1	1760:1776	glycosylated IgG1	1760:1776	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	7	17	theme	neutron	914:920	arg1	scattering					922:931	neutron scattering	914:931	neutron scattering	914:931	X-ray and neutron scattering revealed changes in the Guinier structural parameters after deglycosylation.					
33675758	1	18	theme	human	103:107	arg1	IgG					127:129	IgG	127:129	IgG	127:129	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	1	18	theme	human	103:107	arg1	G					124:124	human immunoglobulin G	103:124	The human immunoglobulin G (IgG) class	99:136	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	8	19	theme	P	1209:1209	arg1	M2					1171:1172	the commonly occurring distance peak M2	1134:1172	the commonly occurring distance peak M2 of the distance distribution curve P(r)	1134:1212	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	1	20	theme	G	124:124	arg1	antibody					160:167	the most prevalent antibody	141:167	the most prevalent antibody in serum	141:176	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	1	20	theme	G	124:124	arg1	class					132:136	The human immunoglobulin G (IgG) class	99:136	The human immunoglobulin G (IgG) class	99:136	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	14	21	gly	deglycosylated	2029:2042	arg1	IgG1					2044:2047	deglycosylated IgG1	2029:2047	deglycosylated IgG1	2029:2047	These more variable Fc conformations account for the loss of binding to the Fcγ receptor in deglycosylated IgG1.					
33675758	13	22	theme	relative	1821:1828	arg1	conformations					1807:1819	conformations	1807:1819	conformations relative to the Fab regions	1807:1847	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	0	23	theme	CH2	66:68	arg1	glycans					70:76	CH2 glycans	66:76	CH2 glycans	66:76	Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation.					
33675758	12	24	theme	principal	1601:1609	arg1	analyses					1621:1628	principal component analyses	1601:1628	principal component analyses	1601:1628	For these, principal component analyses identified five groups of structural conformations that were different for glycosylated and deglycosylated IgG1.					
33675758	5	25	theme	peptide	650:656	arg1	F					672:672	peptide:N-glycosidase F	650:672	peptide:N-glycosidase F	650:672	After deglycosylation using peptide:N-glycosidase F, analytical ultracentrifugation showed that IgG1 remained monomeric and the sedimentation coefficients s020,w of IgG1 decreased from 6.45 S by 0.16-0.27 S.					
33675758	14	26	theme	variable	1948:1955	arg1	conformations					1960:1972	These more variable Fc conformations	1937:1972	These more variable Fc conformations	1937:1972	These more variable Fc conformations account for the loss of binding to the Fcγ receptor in deglycosylated IgG1.					
33675758	10	27	theme	scattering	1366:1375	arg1	modeling					1377:1384	atomistic scattering modeling	1356:1384	atomistic scattering modeling based on Monte Carlo simulations	1356:1417	To explain these changes, atomistic scattering modeling based on Monte Carlo simulations resulted in 123,284 and 119,191 trial structures for glycosylated and deglycosylated IgG1 respectively.					
33675758	13	28	theme	restricted	1787:1796	arg1	range					1798:1802	a restricted range	1785:1802	a restricted range of conformations relative to the Fab regions	1785:1847	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	3	29	theme	Fc	363:364	arg1	region					366:371	the Fc region	359:371	the Fc region in IgG	359:378	Two glycan chains are conserved in the Fc region in IgG; however, their importance for the structure of intact IgG1 has remained unclear.					
33675758	5	30	theme	N-glycosidase	658:670	arg1	F					672:672	peptide:N-glycosidase F	650:672	peptide:N-glycosidase F	650:672	After deglycosylation using peptide:N-glycosidase F, analytical ultracentrifugation showed that IgG1 remained monomeric and the sedimentation coefficients s020,w of IgG1 decreased from 6.45 S by 0.16-0.27 S.					
33675758	14	31	theme	Fcγ	2013:2015	arg1	receptor					2017:2024	the Fcγ receptor	2009:2024	the Fcγ receptor	2009:2024	These more variable Fc conformations account for the loss of binding to the Fcγ receptor in deglycosylated IgG1.					
33675758	12	32	dep	glycosylated	1705:1716	arg1	IgG1					1737:1740	IgG1	1737:1740	IgG1	1737:1740	For these, principal component analyses identified five groups of structural conformations that were different for glycosylated and deglycosylated IgG1.					
33675758	0	33	theme	Solution	0:7	arg1	structure					9:17	Solution structure	0:17	Solution structure of deglycosylated human IgG1	0:46	Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation.					
33675758	10	34	theme	Monte	1395:1399	arg1	simulations					1407:1417	Monte Carlo simulations	1395:1417	Monte Carlo simulations	1395:1417	To explain these changes, atomistic scattering modeling based on Monte Carlo simulations resulted in 123,284 and 119,191 trial structures for glycosylated and deglycosylated IgG1 respectively.					
33675758	13	35	theme	glycosylated	1760:1771	arg1	IgG1					1773:1776	glycosylated IgG1	1760:1776	glycosylated IgG1	1760:1776	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	5	36	theme	sedimentation	750:762	arg1	coefficients					764:775	the sedimentation coefficients s020,w	746:782	the sedimentation coefficients s020,w of IgG1	746:790	After deglycosylation using peptide:N-glycosidase F, analytical ultracentrifugation showed that IgG1 remained monomeric and the sedimentation coefficients s020,w of IgG1 decreased from 6.45 S by 0.16-0.27 S.					
33675758	0	37	theme	human	37:41	arg1	IgG1					43:46	deglycosylated human IgG1	22:46	deglycosylated human IgG1	22:46	Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation.					
33675758	3	38	theme	glycan	328:333	arg1	chains					335:340	Two glycan chains	324:340	Two glycan chains	324:340	Two glycan chains are conserved in the Fc region in IgG; however, their importance for the structure of intact IgG1 has remained unclear.					
33675758	2	39	theme	Fc	278:279	arg1	region					281:286	a Fc region	276:286	a Fc region	276:286	IgG1 is composed of two Fab regions connected to a Fc region through a 15-residue hinge peptide.					
33675758	8	40	theme	gyration	1033:1040	arg1	radius					1023:1028	the radius	1019:1028	the radius of gyration (RG)	1019:1045	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	8	40	theme	gyration	1033:1040	arg1	unchanged					1051:1059	unchanged	1051:1059	unchanged	1051:1059	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	0	41	gly	deglycosylated	22:35	arg1	IgG1					43:46	deglycosylated human IgG1	22:46	deglycosylated human IgG1	22:46	Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation.					
33675758	6	42	from	reduction	864:872	arg1	mass					877:880	mass	877:880	mass	877:880	This change was attributed to the reduction in mass after glycan removal.					
33675758	9	43	theme	Fab-Fc	1267:1272	arg1	separation					1274:1283	the Fab-Fc separation	1263:1283	the Fab-Fc separation in IgG1	1263:1291	These changes revealed that the Fab-Fc separation in IgG1 was perturbed after deglycosylation.					
33675758	9	44	from	separation	1274:1283	arg1	IgG1					1288:1291	IgG1	1288:1291	IgG1	1288:1291	These changes revealed that the Fab-Fc separation in IgG1 was perturbed after deglycosylation.					
33675758	4	45	theme	monoclonal	513:522	arg1	IgG1					530:533	monoclonal human IgG1	513:533	glycosylated and deglycosylated monoclonal human IgG1 (designated as A33)	481:553	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	10	46	theme	trial	1451:1455	arg1	structures					1457:1466	123,284 and 119,191 trial structures	1431:1466	123,284 and 119,191 trial structures for glycosylated and deglycosylated IgG1	1431:1507	To explain these changes, atomistic scattering modeling based on Monte Carlo simulations resulted in 123,284 and 119,191 trial structures for glycosylated and deglycosylated IgG1 respectively.					
33675758	5	47	dep	coefficients	764:775	arg1	w					782:782	w	782:782	w	782:782	After deglycosylation using peptide:N-glycosidase F, analytical ultracentrifugation showed that IgG1 remained monomeric and the sedimentation coefficients s020,w of IgG1 decreased from 6.45 S by 0.16-0.27 S.					
33675758	5	47	dep	coefficients	764:775	arg1	s020					777:780	s020	777:780	s020	777:780	After deglycosylation using peptide:N-glycosidase F, analytical ultracentrifugation showed that IgG1 remained monomeric and the sedimentation coefficients s020,w of IgG1 decreased from 6.45 S by 0.16-0.27 S.					
33675758	13	48	theme	conformational	1912:1925	arg1	spectrum					1927:1934	a broader conformational spectrum	1902:1934	a broader conformational spectrum	1902:1934	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	2	49	theme	15-residue	298:307	arg1	peptide					315:321	a 15-residue hinge peptide	296:321	a 15-residue hinge peptide	296:321	IgG1 is composed of two Fab regions connected to a Fc region through a 15-residue hinge peptide.					
33675758	1	50	theme	IgG1	188:191	arg1	subclass					193:200	the IgG1 subclass	184:200	the IgG1 subclass	184:200	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	1	50	theme	IgG1	188:191	arg1	abundant					217:224	abundant	217:224	abundant	217:224	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	13	51	theme	Fc	1747:1748	arg1	region					1750:1755	The Fc region	1743:1755	The Fc region in glycosylated IgG1	1743:1776	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	5	52	theme	analytical	675:684	arg1	ultracentrifugation					686:704	analytical ultracentrifugation	675:704	analytical ultracentrifugation	675:704	After deglycosylation using peptide:N-glycosidase F, analytical ultracentrifugation showed that IgG1 remained monomeric and the sedimentation coefficients s020,w of IgG1 decreased from 6.45 S by 0.16-0.27 S.					
33675758	3	53	from	region	366:371	arg1	IgG					376:378	IgG	376:378	IgG	376:378	Two glycan chains are conserved in the Fc region in IgG; however, their importance for the structure of intact IgG1 has remained unclear.					
33675758	8	54	theme	cross-sectional	1066:1080	arg1	radius					1082:1087	the cross-sectional radius	1062:1087	the cross-sectional radius of gyration (RXS-1)	1062:1107	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	3	55	theme	IgG1	435:438	arg1	structure					415:423	the structure	411:423	the structure of intact IgG1	411:438	Two glycan chains are conserved in the Fc region in IgG; however, their importance for the structure of intact IgG1 has remained unclear.					
33675758	4	56	theme	multidisciplinary	572:588	arg1	study					601:605	a comparative multidisciplinary structural study	558:605	a comparative multidisciplinary structural study of both forms	558:619	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	13	57	theme	Fc	1862:1863	arg1	region					1865:1870	the Fc region	1858:1870	the Fc region in deglycosylated IgG1	1858:1893	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	13	58	gly	deglycosylated	1875:1888	arg1	IgG1					1890:1893	deglycosylated IgG1	1875:1893	deglycosylated IgG1	1875:1893	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	6	59	theme	glycan	888:893	arg1	removal					895:901	glycan removal	888:901	glycan removal	888:901	This change was attributed to the reduction in mass after glycan removal.					
33675758	12	60	theme	conformations	1667:1679	arg1	groups					1646:1651	five groups	1641:1651	five groups of structural conformations that were different for glycosylated and deglycosylated IgG1	1641:1740	For these, principal component analyses identified five groups of structural conformations that were different for glycosylated and deglycosylated IgG1.					
33675758	12	60	theme	conformations	1667:1679	arg1	conformations					1667:1679	structural conformations	1656:1679	structural conformations	1656:1679	For these, principal component analyses identified five groups of structural conformations that were different for glycosylated and deglycosylated IgG1.					
33675758	8	61	theme	occurring	1147:1155	arg1	M2					1171:1172	the commonly occurring distance peak M2	1134:1172	the commonly occurring distance peak M2 of the distance distribution curve P(r)	1134:1212	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	8	62	theme	peak	1166:1169	arg1	M2					1171:1172	the commonly occurring distance peak M2	1134:1172	the commonly occurring distance peak M2 of the distance distribution curve P(r)	1134:1212	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	4	63	theme	comparative	560:570	arg1	study					601:605	a comparative multidisciplinary structural study	558:605	a comparative multidisciplinary structural study of both forms	558:619	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	7	64	from	changes	942:948	arg1	parameters					976:985	the Guinier structural parameters	953:985	the Guinier structural parameters	953:985	X-ray and neutron scattering revealed changes in the Guinier structural parameters after deglycosylation.					
33675758	7	65	theme	Guinier	957:963	arg1	parameters					976:985	the Guinier structural parameters	953:985	the Guinier structural parameters	953:985	X-ray and neutron scattering revealed changes in the Guinier structural parameters after deglycosylation.					
33675758	13	66	from	region	1865:1870	arg1	IgG1					1890:1893	deglycosylated IgG1	1875:1893	deglycosylated IgG1	1875:1893	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	8	67	theme	distance	1181:1188	arg1	r					1211:1211	r	1211:1211	r	1211:1211	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	8	67	theme	distance	1181:1188	arg1	P					1209:1209	the distance distribution curve P	1177:1209	the distance distribution curve P(r)	1177:1212	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	13	68	theme	Fab	1837:1839	arg1	regions					1841:1847	the Fab regions	1833:1847	the Fab regions	1833:1847	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	4	69	dep	IgG1	530:533	arg1	designated					536:545	designated	536:545	designated as A33	536:552	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	1	70	theme	immunoglobulin	109:122	arg1	IgG					127:129	IgG	127:129	IgG	127:129	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	1	70	theme	immunoglobulin	109:122	arg1	G					124:124	human immunoglobulin G	103:124	The human immunoglobulin G (IgG) class	99:136	The human immunoglobulin G (IgG) class is the most prevalent antibody in serum, with the IgG1 subclass being the most abundant.					
33675758	12	71	theme	component	1611:1619	arg1	analyses					1621:1628	principal component analyses	1601:1628	principal component analyses	1601:1628	For these, principal component analyses identified five groups of structural conformations that were different for glycosylated and deglycosylated IgG1.					
33675758	8	72	theme	curve	1203:1207	arg1	r					1211:1211	r	1211:1211	r	1211:1211	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	8	72	theme	curve	1203:1207	arg1	P					1209:1209	the distance distribution curve P	1177:1209	the distance distribution curve P(r)	1177:1212	Although the radius of gyration (RG) was unchanged, the cross-sectional radius of gyration (RXS-1) increased by 0.1 nm, and the commonly occurring distance peak M2 of the distance distribution curve P(r) increased by 0.4 nm.					
33675758	4	73	theme	structural	590:599	arg1	study					601:605	a comparative multidisciplinary structural study	558:605	a comparative multidisciplinary structural study of both forms	558:619	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	13	74	theme	conformations	1807:1819	arg1	range					1798:1802	a restricted range	1785:1802	a restricted range of conformations relative to the Fab regions	1785:1847	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	0	75	theme	glycans	70:76	arg1	role					58:61	the role	54:61	the role of CH2 glycans in its conformation	54:96	Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation.					
33675758	14	76	theme	Fc	1957:1958	arg1	conformations					1960:1972	These more variable Fc conformations	1937:1972	These more variable Fc conformations	1937:1972	These more variable Fc conformations account for the loss of binding to the Fcγ receptor in deglycosylated IgG1.					
33675758	14	77	theme	deglycosylated	2029:2042	arg1	IgG1					2044:2047	deglycosylated IgG1	2029:2047	deglycosylated IgG1	2029:2047	These more variable Fc conformations account for the loss of binding to the Fcγ receptor in deglycosylated IgG1.					
33675758	10	78	theme	atomistic	1356:1364	arg1	modeling					1377:1384	atomistic scattering modeling	1356:1384	atomistic scattering modeling based on Monte Carlo simulations	1356:1417	To explain these changes, atomistic scattering modeling based on Monte Carlo simulations resulted in 123,284 and 119,191 trial structures for glycosylated and deglycosylated IgG1 respectively.					
33675758	4	79	gly	glycosylated	481:492	arg1	IgG1					530:533	monoclonal human IgG1	513:533	glycosylated and deglycosylated monoclonal human IgG1 (designated as A33)	481:553	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	10	80	gly	deglycosylated	1489:1502	arg1	IgG1					1504:1507	deglycosylated IgG1	1489:1507	deglycosylated IgG1	1489:1507	To explain these changes, atomistic scattering modeling based on Monte Carlo simulations resulted in 123,284 and 119,191 trial structures for glycosylated and deglycosylated IgG1 respectively.					
33675758	2	81	theme	hinge	309:313	arg1	peptide					315:321	a 15-residue hinge peptide	296:321	a 15-residue hinge peptide	296:321	IgG1 is composed of two Fab regions connected to a Fc region through a 15-residue hinge peptide.					
33675758	0	82	theme	deglycosylated	22:35	arg1	IgG1					43:46	deglycosylated human IgG1	22:46	deglycosylated human IgG1	22:46	Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation.					
33675758	4	83	gly	deglycosylated	498:511	arg1	IgG1					530:533	monoclonal human IgG1	513:533	glycosylated and deglycosylated monoclonal human IgG1 (designated as A33)	481:553	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	14	84	theme	binding	1998:2004	arg1	loss					1990:1993	the loss	1986:1993	the loss of binding to the Fcγ receptor in deglycosylated IgG1	1986:2047	These more variable Fc conformations account for the loss of binding to the Fcγ receptor in deglycosylated IgG1.					
33675758	0	85	theme	IgG1	43:46	arg1	structure					9:17	Solution structure	0:17	Solution structure of deglycosylated human IgG1	0:46	Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation.					
33675758	14	86	from	loss	1990:1993	arg1	IgG1					2044:2047	deglycosylated IgG1	2029:2047	deglycosylated IgG1	2029:2047	These more variable Fc conformations account for the loss of binding to the Fcγ receptor in deglycosylated IgG1.					
33675758	13	87	gly	glycosylated	1760:1771	arg1	IgG1					1773:1776	glycosylated IgG1	1760:1776	glycosylated IgG1	1760:1776	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	4	88	theme	human	524:528	arg1	IgG1					530:533	monoclonal human IgG1	513:533	glycosylated and deglycosylated monoclonal human IgG1 (designated as A33)	481:553	Here, we subjected glycosylated and deglycosylated monoclonal human IgG1 (designated as A33) to a comparative multidisciplinary structural study of both forms.					
33675758	10	89	theme	Carlo	1401:1405	arg1	simulations					1407:1417	Monte Carlo simulations	1395:1417	Monte Carlo simulations	1395:1417	To explain these changes, atomistic scattering modeling based on Monte Carlo simulations resulted in 123,284 and 119,191 trial structures for glycosylated and deglycosylated IgG1 respectively.					
33675758	13	90	theme	broader	1904:1910	arg1	spectrum					1927:1934	a broader conformational spectrum	1902:1934	a broader conformational spectrum	1902:1934	The Fc region in glycosylated IgG1 showed a restricted range of conformations relative to the Fab regions, whereas the Fc region in deglycosylated IgG1 showed a broader conformational spectrum.					
33675758	11	91	theme	neutron	1549:1555	arg1	models					1566:1571	neutron best-fit models	1549:1571	neutron best-fit models	1549:1571	From these, 100 x-ray and neutron best-fit models were determined.					
33675758	3	92	theme	intact	428:433	arg1	IgG1					435:438	intact IgG1	428:438	intact IgG1	428:438	Two glycan chains are conserved in the Fc region in IgG; however, their importance for the structure of intact IgG1 has remained unclear.					
32989128	0	0	theme	selective	37:45	arg1	probing					47:53	selective probing	37:53	selective probing of O-linked N-acetylgalactosamine glycosylation	37:101	Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation.					
32989128	10	1	theme	detailed	1835:1842	arg1	view					1844:1847	a detailed view	1833:1847	a detailed view into the biology of a major type of cancer-relevant protein glycosylation	1833:1921	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	9	2	theme	engineered	1535:1544	arg1	glycosyltransferase					1546:1564	an engineered glycosyltransferase	1532:1564	an engineered glycosyltransferase	1532:1564	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2, boosts labeling in a programmable fashion by increasing incorporation of GalNAzMe into the cell surface glycoproteome.					
32989128	6	3	theme	O-GalNAc	971:978	arg1	glycosylation					980:992	O-GalNAc glycosylation	971:992	O-GalNAc glycosylation	971:992	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	3	4	theme	living	473:478	arg1	cell					480:483	the living cell	469:483	the living cell	469:483	However, metabolic interconversion into other monosaccharides drastically reduces such specificity in the living cell.					
32989128	8	5	theme	O-glycan-specific	1328:1344	arg1	reporter					1346:1353	an O-glycan-specific reporter	1325:1353	an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids	1325:1496	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	8	5	theme	O-glycan-specific	1328:1344	arg1	GalNAzMe					1306:1313	GalNAzMe	1306:1313	GalNAzMe	1306:1313	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	7	6	theme	uridine	1194:1200	arg1	UDP					1215:1217	the nucleotide-sugar donor uridine diphosphate (UDP)	1167:1218	the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe	1167:1227	We transfect cells with the engineered pyrophosphorylase mut-AGX1 to biosynthesize the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe from a sugar-1-phosphate precursor.					
32989128	5	7	theme	GalNAc-based	934:945	arg1	probes					947:952	conventional GalNAc-based probes	921:952	conventional GalNAc-based probes	921:952	By virtue of a branched N-acylamide side chain, GalNAzMe is not interconverted by epimerization to the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes.					
32989128	8	8	theme	bioorthogonal	1279:1291	arg1	group					1299:1303	a bioorthogonal azide group	1277:1303	a bioorthogonal azide group	1277:1303	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	7	9	theme	nucleotide-sugar	1171:1186	arg1	UDP					1215:1217	the nucleotide-sugar donor uridine diphosphate (UDP)	1167:1218	the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe	1167:1227	We transfect cells with the engineered pyrophosphorylase mut-AGX1 to biosynthesize the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe from a sugar-1-phosphate precursor.					
32989128	10	10	theme	cancer-relevant	1885:1899	arg1	glycosylation					1909:1921	cancer-relevant protein glycosylation	1885:1921	cancer-relevant protein glycosylation	1885:1921	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	9	11	from	labeling	1606:1613	arg1	fashion					1633:1639	a programmable fashion	1618:1639	a programmable fashion	1618:1639	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2, boosts labeling in a programmable fashion by increasing incorporation of GalNAzMe into the cell surface glycoproteome.					
32989128	4	12	theme	-linked	660:666	arg1	glycosylation					699:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	5	13	theme	N-acylamide	738:748	arg1	chain					755:759	a branched N-acylamide side chain	727:759	a branched N-acylamide side chain	727:759	By virtue of a branched N-acylamide side chain, GalNAzMe is not interconverted by epimerization to the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes.					
32989128	10	14	theme	type	1877:1880	arg1	biology					1858:1864	the biology	1854:1864	the biology of a major type of cancer-relevant protein glycosylation	1854:1921	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	6	15	theme	glycan	1038:1043	arg1	types					1045:1049	other major cell surface glycan types	1013:1049	other major cell surface glycan types including Asn(N)-linked glycans	1013:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	6	15	theme	glycan	1038:1043	arg1	glycans					1075:1081	Asn(N)-linked glycans	1061:1081	Asn(N)-linked glycans	1061:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	8	16	theme	chemical	1386:1393	arg1	glycoproteomics					1395:1409	chemical glycoproteomics	1386:1409	chemical glycoproteomics	1386:1409	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	5	17	theme	chain	755:759	arg1	virtue					717:722	virtue	717:722	virtue of a branched N-acylamide side chain	717:759	By virtue of a branched N-acylamide side chain, GalNAzMe is not interconverted by epimerization to the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes.					
32989128	6	18	theme	cell	1025:1028	arg1	types					1045:1049	other major cell surface glycan types	1013:1049	other major cell surface glycan types including Asn(N)-linked glycans	1013:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	6	18	theme	cell	1025:1028	arg1	glycans					1075:1081	Asn(N)-linked glycans	1061:1081	Asn(N)-linked glycans	1061:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	7	19	theme	engineered	1112:1121	arg1	mut-AGX1					1141:1148	the engineered pyrophosphorylase mut-AGX1	1108:1148	the engineered pyrophosphorylase mut-AGX1	1108:1148	We transfect cells with the engineered pyrophosphorylase mut-AGX1 to biosynthesize the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe from a sugar-1-phosphate precursor.					
32989128	10	20	theme	glycosylation	1909:1921	arg1	type					1877:1880	a major type	1869:1880	a major type of cancer-relevant protein glycosylation	1869:1921	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	6	21	theme	other	1013:1017	arg1	types					1045:1049	other major cell surface glycan types	1013:1049	other major cell surface glycan types including Asn(N)-linked glycans	1013:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	6	21	theme	other	1013:1017	arg1	glycans					1075:1081	Asn(N)-linked glycans	1061:1081	Asn(N)-linked glycans	1061:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	8	22	theme	genome-wide	1414:1424	arg1	screen					1454:1459	a genome-wide CRISPR-knockout (CRISPR-KO) screen	1412:1459	a genome-wide CRISPR-knockout (CRISPR-KO) screen	1412:1459	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	1	23	theme	secretory	158:166	arg1	pathway					168:174	the secretory pathway	154:174	the secretory pathway	154:174	Protein glycosylation events that happen early in the secretory pathway are often dysregulated during tumorigenesis.					
32989128	4	24	link	-linked	660:666	arg1	glycosylation					699:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	0	25	theme	N-acetylgalactosamine	67:87	arg1	glycosylation					89:101	O-linked N-acetylgalactosamine glycosylation	58:101	O-linked N-acetylgalactosamine glycosylation	58:101	Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation.					
32989128	4	26	theme	monosaccharide	547:560	arg1	specific					621:628	specific	621:628	specific	621:628	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	4	26	theme	monosaccharide	547:560	arg1	GalNAzMe					603:610	GalNAzMe	603:610	GalNAzMe	603:610	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	4	26	theme	monosaccharide	547:560	arg1	-azidopropionylgalactosamine					573:600	the monosaccharide probe N-(S)-azidopropionylgalactosamine	543:600	the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	543:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	4	27	theme	structure-based	501:515	arg1	process					524:530	a structure-based design process	499:530	a structure-based design process	499:530	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	1	28	theme	Protein	104:110	arg1	events					126:131	Protein glycosylation events	104:131	Protein glycosylation events that happen early in the secretory pathway	104:174	Protein glycosylation events that happen early in the secretory pathway are often dysregulated during tumorigenesis.					
32989128	8	29	theme	intestinal	1477:1486	arg1	organoids					1488:1496	intestinal organoids	1477:1496	intestinal organoids	1477:1496	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	10	30	theme	labeling	1770:1777	arg1	experiments					1779:1789	metabolic labeling experiments	1760:1789	metabolic labeling experiments	1760:1789	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	0	31	theme	Metabolic	0:8	arg1	labeling					20:27	Metabolic precision labeling	0:27	Metabolic precision labeling	0:27	Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation.					
32989128	9	32	theme	cell	1690:1693	arg1	glycoproteome					1703:1715	the cell surface glycoproteome	1686:1715	the cell surface glycoproteome	1686:1715	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2, boosts labeling in a programmable fashion by increasing incorporation of GalNAzMe into the cell surface glycoproteome.					
32989128	3	33	theme	other	407:411	arg1	monosaccharides					413:427	other monosaccharides	407:427	other monosaccharides	407:427	However, metabolic interconversion into other monosaccharides drastically reduces such specificity in the living cell.					
32989128	10	34	theme	precision	1806:1814	arg1	tool					1816:1819	a precision tool	1804:1819	a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation	1804:1921	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	10	34	theme	precision	1806:1814	arg1	GalNAzMe					1792:1799	GalNAzMe	1792:1799	GalNAzMe	1792:1799	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	5	35	theme	corresponding	817:829	arg1	analog					851:856	the corresponding N-acetylglucosamine analog	813:856	the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes	813:952	By virtue of a branched N-acylamide side chain, GalNAzMe is not interconverted by epimerization to the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes.					
32989128	7	36	theme	sugar-1-phosphate	1236:1252	arg1	precursor					1254:1262	a sugar-1-phosphate precursor	1234:1262	a sugar-1-phosphate precursor	1234:1262	We transfect cells with the engineered pyrophosphorylase mut-AGX1 to biosynthesize the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe from a sugar-1-phosphate precursor.					
32989128	3	37	theme	such	449:452	arg1	specificity					454:464	such specificity	449:464	such specificity	449:464	However, metabolic interconversion into other monosaccharides drastically reduces such specificity in the living cell.					
32989128	4	38	theme	N-	568:569	arg1	specific					621:628	specific	621:628	specific	621:628	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	4	38	theme	N-	568:569	arg1	GalNAzMe					603:610	GalNAzMe	603:610	GalNAzMe	603:610	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	4	38	theme	N-	568:569	arg1	-azidopropionylgalactosamine					573:600	the monosaccharide probe N-(S)-azidopropionylgalactosamine	543:600	the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	543:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	4	39	theme	S	571:571	arg1	specific					621:628	specific	621:628	specific	621:628	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	4	39	theme	S	571:571	arg1	GalNAzMe					603:610	GalNAzMe	603:610	GalNAzMe	603:610	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	4	39	theme	S	571:571	arg1	-azidopropionylgalactosamine					573:600	the monosaccharide probe N-(S)-azidopropionylgalactosamine	543:600	the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	543:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	9	40	theme	Additional	1499:1508	arg1	expression					1518:1527	Additional ectopic expression	1499:1527	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2,	1499:1597	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2, boosts labeling in a programmable fashion by increasing incorporation of GalNAzMe into the cell surface glycoproteome.					
32989128	4	41	theme	cancer-relevant	634:648	arg1	glycosylation					699:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	0	42	theme	O-linked	58:65	arg1	glycosylation					89:101	O-linked N-acetylgalactosamine glycosylation	58:101	O-linked N-acetylgalactosamine glycosylation	58:101	Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation.					
32989128	7	43	theme	UDP	1215:1217	arg1	-GalNAzMe					1219:1227	the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe	1167:1227	the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe	1167:1227	We transfect cells with the engineered pyrophosphorylase mut-AGX1 to biosynthesize the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe from a sugar-1-phosphate precursor.					
32989128	7	44	theme	diphosphate	1202:1212	arg1	UDP					1215:1217	the nucleotide-sugar donor uridine diphosphate (UDP)	1167:1218	the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe	1167:1227	We transfect cells with the engineered pyrophosphorylase mut-AGX1 to biosynthesize the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe from a sugar-1-phosphate precursor.					
32989128	10	45	theme	major	1871:1875	arg1	type					1877:1880	a major type	1869:1880	a major type of cancer-relevant protein glycosylation	1869:1921	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	9	46	theme	glycosyltransferase	1546:1564	arg1	expression					1518:1527	Additional ectopic expression	1499:1527	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2,	1499:1597	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2, boosts labeling in a programmable fashion by increasing incorporation of GalNAzMe into the cell surface glycoproteome.					
32989128	2	47	theme	distinct	341:348	arg1	subtypes					357:364	distinct glycan subtypes	341:364	distinct glycan subtypes	341:364	These events can be probed, in principle, by monosaccharides with bioorthogonal tags that would ideally be specific for distinct glycan subtypes.					
32989128	7	48	theme	donor	1188:1192	arg1	UDP					1215:1217	the nucleotide-sugar donor uridine diphosphate (UDP)	1167:1218	the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe	1167:1227	We transfect cells with the engineered pyrophosphorylase mut-AGX1 to biosynthesize the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe from a sugar-1-phosphate precursor.					
32989128	5	49	theme	conventional	921:932	arg1	probes					947:952	conventional GalNAc-based probes	921:952	conventional GalNAc-based probes	921:952	By virtue of a branched N-acylamide side chain, GalNAzMe is not interconverted by epimerization to the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes.					
32989128	0	50	link	O-linked	58:65	arg1	glycosylation					89:101	O-linked N-acetylgalactosamine glycosylation	58:101	O-linked N-acetylgalactosamine glycosylation	58:101	Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation.					
32989128	4	51	theme	GalNAc	691:696	arg1	glycosylation					699:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	10	52	theme	protein	1901:1907	arg1	glycosylation					1909:1921	cancer-relevant protein glycosylation	1885:1921	cancer-relevant protein glycosylation	1885:1921	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	5	53	theme	branched	729:736	arg1	chain					755:759	a branched N-acylamide side chain	727:759	a branched N-acylamide side chain	727:759	By virtue of a branched N-acylamide side chain, GalNAzMe is not interconverted by epimerization to the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes.					
32989128	6	54	link	-linked	1067:1073	arg1	glycans					1075:1081	Asn(N)-linked glycans	1061:1081	Asn(N)-linked glycans	1061:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	8	55	theme	azide	1293:1297	arg1	group					1299:1303	a bioorthogonal azide group	1277:1303	a bioorthogonal azide group	1277:1303	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	8	56	theme	superresolution	1358:1372	arg1	microscopy					1374:1383	superresolution microscopy	1358:1383	superresolution microscopy	1358:1383	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	7	57	theme	pyrophosphorylase	1123:1139	arg1	mut-AGX1					1141:1148	the engineered pyrophosphorylase mut-AGX1	1108:1148	the engineered pyrophosphorylase mut-AGX1	1108:1148	We transfect cells with the engineered pyrophosphorylase mut-AGX1 to biosynthesize the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe from a sugar-1-phosphate precursor.					
32989128	9	58	theme	-GalNAc-T2	1587:1596	arg1	glycosyltransferase					1546:1564	an engineered glycosyltransferase	1532:1564	an engineered glycosyltransferase	1532:1564	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2, boosts labeling in a programmable fashion by increasing incorporation of GalNAzMe into the cell surface glycoproteome.					
32989128	2	59	with	monosaccharides	266:280	arg1	tags					301:304	bioorthogonal tags	287:304	bioorthogonal tags that would ideally be specific for distinct glycan subtypes	287:364	These events can be probed, in principle, by monosaccharides with bioorthogonal tags that would ideally be specific for distinct glycan subtypes.					
32989128	4	60	theme	N-acetylgalactosamine	668:688	arg1	glycosylation					699:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	10	61	theme	GALE-KO	1743:1749	arg1	cells					1751:1755	GALE-KO cells	1743:1755	GALE-KO cells	1743:1755	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	8	62	from	reporter	1346:1353	arg1	microscopy					1374:1383	superresolution microscopy	1358:1383	superresolution microscopy	1358:1383	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	8	62	from	reporter	1346:1353	arg1	glycoproteomics					1395:1409	chemical glycoproteomics	1386:1409	chemical glycoproteomics	1386:1409	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	8	62	from	reporter	1346:1353	arg1	imaging					1466:1472	imaging	1466:1472	imaging of intestinal organoids	1466:1496	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	8	62	from	reporter	1346:1353	arg1	screen					1454:1459	a genome-wide CRISPR-knockout (CRISPR-KO) screen	1412:1459	a genome-wide CRISPR-knockout (CRISPR-KO) screen	1412:1459	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	6	63	theme	major	1019:1023	arg1	types					1045:1049	other major cell surface glycan types	1013:1049	other major cell surface glycan types including Asn(N)-linked glycans	1013:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	6	63	theme	major	1019:1023	arg1	glycans					1075:1081	Asn(N)-linked glycans	1061:1081	Asn(N)-linked glycans	1061:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	2	64	theme	bioorthogonal	287:299	arg1	tags					301:304	bioorthogonal tags	287:304	bioorthogonal tags that would ideally be specific for distinct glycan subtypes	287:364	These events can be probed, in principle, by monosaccharides with bioorthogonal tags that would ideally be specific for distinct glycan subtypes.					
32989128	0	65	theme	glycosylation	89:101	arg1	probing					47:53	selective probing	37:53	selective probing of O-linked N-acetylgalactosamine glycosylation	37:101	Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation.					
32989128	9	66	theme	programmable	1620:1631	arg1	fashion					1633:1639	a programmable fashion	1618:1639	a programmable fashion	1618:1639	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2, boosts labeling in a programmable fashion by increasing incorporation of GalNAzMe into the cell surface glycoproteome.					
32989128	8	67	theme	CRISPR-knockout	1426:1440	arg1	screen					1454:1459	a genome-wide CRISPR-knockout (CRISPR-KO) screen	1412:1459	a genome-wide CRISPR-knockout (CRISPR-KO) screen	1412:1459	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	6	68	theme	-linked	1067:1073	arg1	glycans					1075:1081	Asn(N)-linked glycans	1061:1081	Asn(N)-linked glycans	1061:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	4	69	theme	probe	562:566	arg1	specific					621:628	specific	621:628	specific	621:628	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	4	69	theme	probe	562:566	arg1	GalNAzMe					603:610	GalNAzMe	603:610	GalNAzMe	603:610	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	4	69	theme	probe	562:566	arg1	-azidopropionylgalactosamine					573:600	the monosaccharide probe N-(S)-azidopropionylgalactosamine	543:600	the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	543:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	8	70	theme	CRISPR-KO	1443:1451	arg1	screen					1454:1459	a genome-wide CRISPR-knockout (CRISPR-KO) screen	1412:1459	a genome-wide CRISPR-knockout (CRISPR-KO) screen	1412:1459	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	4	71	theme	design	517:522	arg1	process					524:530	a structure-based design process	499:530	a structure-based design process	499:530	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	9	72	theme	GalNAzMe	1672:1679	arg1	incorporation					1655:1667	incorporation	1655:1667	incorporation of GalNAzMe into the cell surface glycoproteome	1655:1715	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2, boosts labeling in a programmable fashion by increasing incorporation of GalNAzMe into the cell surface glycoproteome.					
32989128	7	73	with	cells	1097:1101	arg1	mut-AGX1					1141:1148	the engineered pyrophosphorylase mut-AGX1	1108:1148	the engineered pyrophosphorylase mut-AGX1	1108:1148	We transfect cells with the engineered pyrophosphorylase mut-AGX1 to biosynthesize the nucleotide-sugar donor uridine diphosphate (UDP)-GalNAzMe from a sugar-1-phosphate precursor.					
32989128	3	74	theme	metabolic	376:384	arg1	interconversion					386:400	metabolic interconversion	376:400	metabolic interconversion into other monosaccharides	376:427	However, metabolic interconversion into other monosaccharides drastically reduces such specificity in the living cell.					
32989128	0	75	theme	precision	10:18	arg1	labeling					20:27	Metabolic precision labeling	0:27	Metabolic precision labeling	0:27	Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation.					
32989128	1	76	theme	glycosylation	112:124	arg1	events					126:131	Protein glycosylation events	104:131	Protein glycosylation events that happen early in the secretory pathway	104:174	Protein glycosylation events that happen early in the secretory pathway are often dysregulated during tumorigenesis.					
32989128	5	77	theme	side	750:753	arg1	chain					755:759	a branched N-acylamide side chain	727:759	a branched N-acylamide side chain	727:759	By virtue of a branched N-acylamide side chain, GalNAzMe is not interconverted by epimerization to the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes.					
32989128	10	78	theme	metabolic	1760:1768	arg1	experiments					1779:1789	metabolic labeling experiments	1760:1789	metabolic labeling experiments	1760:1789	Alleviating the need for GALE-KO cells in metabolic labeling experiments, GalNAzMe is a precision tool that allows a detailed view into the biology of a major type of cancer-relevant protein glycosylation.					
32989128	5	79	theme	epimerase	865:873	arg1	GALE					910:913	GALE	910:913	GALE	910:913	By virtue of a branched N-acylamide side chain, GalNAzMe is not interconverted by epimerization to the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes.					
32989128	5	79	theme	epimerase	865:873	arg1	N-acetylgalactosamine-4-epimerase					875:907	the epimerase N-acetylgalactosamine-4-epimerase	861:907	the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes	861:952	By virtue of a branched N-acylamide side chain, GalNAzMe is not interconverted by epimerization to the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes.					
32989128	9	80	theme	surface	1695:1701	arg1	glycoproteome					1703:1715	the cell surface glycoproteome	1686:1715	the cell surface glycoproteome	1686:1715	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2, boosts labeling in a programmable fashion by increasing incorporation of GalNAzMe into the cell surface glycoproteome.					
32989128	8	81	theme	organoids	1488:1496	arg1	microscopy					1374:1383	superresolution microscopy	1358:1383	superresolution microscopy	1358:1383	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	8	81	theme	organoids	1488:1496	arg1	screen					1454:1459	a genome-wide CRISPR-knockout (CRISPR-KO) screen	1412:1459	a genome-wide CRISPR-knockout (CRISPR-KO) screen	1412:1459	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	8	81	theme	organoids	1488:1496	arg1	imaging					1466:1472	imaging	1466:1472	imaging of intestinal organoids	1466:1496	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	8	81	theme	organoids	1488:1496	arg1	glycoproteomics					1395:1409	chemical glycoproteomics	1386:1409	chemical glycoproteomics	1386:1409	Tagged with a bioorthogonal azide group, GalNAzMe serves as an O-glycan-specific reporter in superresolution microscopy, chemical glycoproteomics, a genome-wide CRISPR-knockout (CRISPR-KO) screen, and imaging of intestinal organoids.					
32989128	5	82	theme	N-acetylglucosamine	831:849	arg1	analog					851:856	the corresponding N-acetylglucosamine analog	813:856	the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes	813:952	By virtue of a branched N-acylamide side chain, GalNAzMe is not interconverted by epimerization to the corresponding N-acetylglucosamine analog by the epimerase N-acetylgalactosamine-4-epimerase (GALE) like conventional GalNAc-based probes.					
32989128	6	83	theme	surface	1030:1036	arg1	types					1045:1049	other major cell surface glycan types	1013:1049	other major cell surface glycan types including Asn(N)-linked glycans	1013:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	6	83	theme	surface	1030:1036	arg1	glycans					1075:1081	Asn(N)-linked glycans	1061:1081	Asn(N)-linked glycans	1061:1081	GalNAzMe enters O-GalNAc glycosylation but does not enter other major cell surface glycan types including Asn(N)-linked glycans.					
32989128	4	84	theme	Ser/Thr	650:656	arg1	glycosylation					699:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
32989128	2	85	theme	glycan	350:355	arg1	subtypes					357:364	distinct glycan subtypes	341:364	distinct glycan subtypes	341:364	These events can be probed, in principle, by monosaccharides with bioorthogonal tags that would ideally be specific for distinct glycan subtypes.					
32989128	9	86	theme	ectopic	1510:1516	arg1	expression					1518:1527	Additional ectopic expression	1499:1527	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2,	1499:1597	Additional ectopic expression of an engineered glycosyltransferase, "bump-and-hole" (BH)-GalNAc-T2, boosts labeling in a programmable fashion by increasing incorporation of GalNAzMe into the cell surface glycoproteome.					
32989128	4	87	theme	O	658:658	arg1	glycosylation					699:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation	634:711	Here, we use a structure-based design process to develop the monosaccharide probe N-(S)-azidopropionylgalactosamine (GalNAzMe) that is specific for cancer-relevant Ser/Thr(O)-linked N-acetylgalactosamine (GalNAc) glycosylation.					
33524390	2	0	theme	endoplasmic	403:413	arg1	ER					426:427	ER	426:427	ER	426:427	Glycosyltransferases primarily reside and function in the endoplasmic reticulum (ER) and the Golgi apparatus; whether and how they traffic beyond the Golgi, how this trafficking is controlled, and how it impacts glycosylation remain unclear.					
33524390	2	0	theme	endoplasmic	403:413	arg1	reticulum					415:423	the endoplasmic reticulum	399:423	the endoplasmic reticulum (ER)	399:428	Glycosyltransferases primarily reside and function in the endoplasmic reticulum (ER) and the Golgi apparatus; whether and how they traffic beyond the Golgi, how this trafficking is controlled, and how it impacts glycosylation remain unclear.					
33524390	8	1	from	puncta	1584:1589	arg1	cells					1602:1606	control cells	1594:1606	control cells	1594:1606	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	1	2	theme	glycosyltransferase	268:286	arg1	location					288:295	subcellular glycosyltransferase location	256:295	subcellular glycosyltransferase location	256:295	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	6	3	gly	glycoproteins	1144:1156	arg1	glycoproteins					1144:1156	glycoproteins	1144:1156	glycoproteins	1144:1156	Structural analyses of glycans using lectins and LC-MS revealed that α2,3-sialylation is selectively enhanced, suggesting that an α2,3-sialyltransferase that catalyzes the sialyation of glycoproteins is activated or upregulated as the result of Rab11 knockdown.					
33524390	1	4	theme	proteins	189:196	arg1	modification					173:184	the most common posttranslational modification	139:184	the most common posttranslational modification of proteins	139:196	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	1	4	theme	proteins	189:196	arg1	Glycosylation					124:136	Glycosylation	124:136	Glycosylation	124:136	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	8	5	theme	Rab11	1706:1710	arg1	knockdown					1712:1720	Rab11 knockdown	1706:1720	Rab11 knockdown	1706:1720	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	1	6	theme	location	288:295	arg1	regulation					242:251	tight regulation	236:251	tight regulation of subcellular glycosyltransferase location	236:295	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	7	7	theme	major	1235:1239	arg1	α2,3-sialyltransferase					1241:1262	the major α2,3-sialyltransferase	1231:1262	the major α2,3-sialyltransferase that acts on N-glycans	1231:1285	ST3GAL4 is the major α2,3-sialyltransferase that acts on N-glycans; we demonstrated that the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate, was altered by Rab11 depletion.					
33524390	7	7	theme	major	1235:1239	arg1	ST3GAL4					1220:1226	ST3GAL4	1220:1226	ST3GAL4	1220:1226	ST3GAL4 is the major α2,3-sialyltransferase that acts on N-glycans; we demonstrated that the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate, was altered by Rab11 depletion.					
33524390	4	8	theme	HeLa	836:839	arg1	cells					841:845	HeLa cells	836:845	HeLa cells	836:845	To learn more about the specific role of Rab11, we knocked down Rab11 in HeLa cells.					
33524390	4	9	theme	specific	787:794	arg1	role					796:799	the specific role	783:799	the specific role of Rab11	783:808	To learn more about the specific role of Rab11, we knocked down Rab11 in HeLa cells.					
33524390	8	10	theme	wider	1528:1532	arg1	distribution					1534:1545	the wider distribution	1524:1545	the wider distribution in the Golgi and in other peripheral puncta in control cells	1524:1606	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	8	11	dep	distributed	1470:1480	arg1	compared					1510:1517	compared	1510:1517	compared with the wider distribution in the Golgi and in other peripheral puncta in control cells	1510:1606	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	8	11	dep	distributed	1470:1480	arg1	whereas					1609:1615	whereas	1609:1615	whereas	1609:1615	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	9	12	theme	post-Golgi	1817:1826	arg1	mechanism					1866:1874	a novel mechanism	1858:1874	a novel mechanism of glycosyltransferase regulation	1858:1908	This indicates that Rab11 may negatively regulate α2,3-sialylation by transporting ST3GAL4 to post-Golgi compartments (PGCs), which is a novel mechanism of glycosyltransferase regulation.					
33524390	9	12	theme	post-Golgi	1817:1826	arg1	PGCs					1842:1845	PGCs	1842:1845	PGCs	1842:1845	This indicates that Rab11 may negatively regulate α2,3-sialylation by transporting ST3GAL4 to post-Golgi compartments (PGCs), which is a novel mechanism of glycosyltransferase regulation.					
33524390	9	12	theme	post-Golgi	1817:1826	arg1	compartments					1828:1839	post-Golgi compartments	1817:1839	post-Golgi compartments (PGCs)	1817:1846	This indicates that Rab11 may negatively regulate α2,3-sialylation by transporting ST3GAL4 to post-Golgi compartments (PGCs), which is a novel mechanism of glycosyltransferase regulation.					
33524390	6	13	theme	knockdown	1209:1217	arg1	result					1193:1198	the result	1189:1198	the result of Rab11 knockdown	1189:1217	Structural analyses of glycans using lectins and LC-MS revealed that α2,3-sialylation is selectively enhanced, suggesting that an α2,3-sialyltransferase that catalyzes the sialyation of glycoproteins is activated or upregulated as the result of Rab11 knockdown.					
33524390	6	13	theme	knockdown	1209:1217	arg1	α2,3-sialyltransferase					1088:1109	an α2,3-sialyltransferase	1085:1109	an α2,3-sialyltransferase that catalyzes the sialyation of glycoproteins	1085:1156	Structural analyses of glycans using lectins and LC-MS revealed that α2,3-sialylation is selectively enhanced, suggesting that an α2,3-sialyltransferase that catalyzes the sialyation of glycoproteins is activated or upregulated as the result of Rab11 knockdown.					
33524390	7	14	theme	levels	1350:1355	arg1	localization					1313:1324	the localization	1309:1324	the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate,	1309:1397	ST3GAL4 is the major α2,3-sialyltransferase that acts on N-glycans; we demonstrated that the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate, was altered by Rab11 depletion.					
33524390	3	15	from	player	670:675	arg1	transport					695:703	the post-Golgi transport	680:703	the post-Golgi transport	680:703	Our previous work identified a connection between N-glycosylation and Rab11, a key player in the post-Golgi transport that connects recycling endosomes and other compartments.					
33524390	8	16	theme	α2,6-sialyltransferase	1621:1642	arg1	ST6GAL1					1644:1650	the α2,6-sialyltransferase ST6GAL1	1617:1650	the α2,6-sialyltransferase ST6GAL1	1617:1650	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	6	17	theme	Rab11	1203:1207	arg1	knockdown					1209:1217	Rab11 knockdown	1203:1217	Rab11 knockdown	1203:1217	Structural analyses of glycans using lectins and LC-MS revealed that α2,3-sialylation is selectively enhanced, suggesting that an α2,3-sialyltransferase that catalyzes the sialyation of glycoproteins is activated or upregulated as the result of Rab11 knockdown.					
33524390	7	18	theme	mRNA	1364:1367	arg1	levels					1350:1355	the levels	1346:1355	the levels of its mRNA, protein, or donor substrate	1346:1396	ST3GAL4 is the major α2,3-sialyltransferase that acts on N-glycans; we demonstrated that the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate, was altered by Rab11 depletion.					
33524390	0	19	theme	post-Golgi	15:24	arg1	transport					26:34	Rab11-mediated post-Golgi transport	0:34	Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4	0:67	Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.					
33524390	3	20	theme	other	743:747	arg1	compartments					749:760	other compartments	743:760	other compartments	743:760	Our previous work identified a connection between N-glycosylation and Rab11, a key player in the post-Golgi transport that connects recycling endosomes and other compartments.					
33524390	7	21	theme	Rab11	1414:1418	arg1	depletion					1420:1428	Rab11 depletion	1414:1428	Rab11 depletion	1414:1428	ST3GAL4 is the major α2,3-sialyltransferase that acts on N-glycans; we demonstrated that the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate, was altered by Rab11 depletion.					
33524390	5	22	theme	Rab11	875:879	arg1	knockdown					881:889	Rab11 knockdown	875:889	Rab11 knockdown	875:889	Our findings indicate that Rab11 knockdown results in a dramatic enhancement in the sialylation of N-glycans.					
33524390	0	23	theme	Rab11-mediated	0:13	arg1	transport					26:34	Rab11-mediated post-Golgi transport	0:34	Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4	0:67	Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.					
33524390	5	24	from	enhancement	913:923	arg1	sialylation					932:942	the sialylation	928:942	the sialylation of N-glycans	928:955	Our findings indicate that Rab11 knockdown results in a dramatic enhancement in the sialylation of N-glycans.					
33524390	3	25	theme	previous	591:598	arg1	work					600:603	Our previous work	587:603	Our previous work	587:603	Our previous work identified a connection between N-glycosylation and Rab11, a key player in the post-Golgi transport that connects recycling endosomes and other compartments.					
33524390	1	26	theme	stepwise	204:211	arg1	process					213:219	a stepwise process	202:219	a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide	202:342	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	1	26	theme	stepwise	204:211	arg1	Glycosylation					124:136	Glycosylation	124:136	Glycosylation	124:136	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	7	27	theme	donor	1382:1386	arg1	substrate					1388:1396	donor substrate	1382:1396	donor substrate	1382:1396	ST3GAL4 is the major α2,3-sialyltransferase that acts on N-glycans; we demonstrated that the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate, was altered by Rab11 depletion.					
33524390	6	28	theme	glycans	981:987	arg1	analyses					969:976	Structural analyses	958:976	Structural analyses of glycans using lectins and LC-MS	958:1011	Structural analyses of glycans using lectins and LC-MS revealed that α2,3-sialylation is selectively enhanced, suggesting that an α2,3-sialyltransferase that catalyzes the sialyation of glycoproteins is activated or upregulated as the result of Rab11 knockdown.					
33524390	8	29	theme	other	1567:1571	arg1	puncta					1584:1589	other peripheral puncta	1567:1589	other peripheral puncta in control cells	1567:1606	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	9	30	theme	novel	1860:1864	arg1	mechanism					1866:1874	a novel mechanism	1858:1874	a novel mechanism of glycosyltransferase regulation	1858:1908	This indicates that Rab11 may negatively regulate α2,3-sialylation by transporting ST3GAL4 to post-Golgi compartments (PGCs), which is a novel mechanism of glycosyltransferase regulation.					
33524390	9	30	theme	novel	1860:1864	arg1	compartments					1828:1839	post-Golgi compartments	1817:1839	post-Golgi compartments (PGCs)	1817:1846	This indicates that Rab11 may negatively regulate α2,3-sialylation by transporting ST3GAL4 to post-Golgi compartments (PGCs), which is a novel mechanism of glycosyltransferase regulation.					
33524390	5	31	theme	N-glycans	947:955	arg1	sialylation					932:942	the sialylation	928:942	the sialylation of N-glycans	928:955	Our findings indicate that Rab11 knockdown results in a dramatic enhancement in the sialylation of N-glycans.					
33524390	3	32	theme	key	666:668	arg1	N-glycosylation					637:651	N-glycosylation	637:651	N-glycosylation	637:651	Our previous work identified a connection between N-glycosylation and Rab11, a key player in the post-Golgi transport that connects recycling endosomes and other compartments.					
33524390	3	32	theme	key	666:668	arg1	player					670:675	a key player	664:675	a key player in the post-Golgi transport that connects recycling endosomes and other compartments	664:760	Our previous work identified a connection between N-glycosylation and Rab11, a key player in the post-Golgi transport that connects recycling endosomes and other compartments.					
33524390	5	33	theme	dramatic	904:911	arg1	enhancement					913:923	a dramatic enhancement	902:923	a dramatic enhancement in the sialylation of N-glycans	902:955	Our findings indicate that Rab11 knockdown results in a dramatic enhancement in the sialylation of N-glycans.					
33524390	6	34	theme	glycoproteins	1144:1156	arg1	sialyation					1130:1139	the sialyation	1126:1139	the sialyation of glycoproteins	1126:1156	Structural analyses of glycans using lectins and LC-MS revealed that α2,3-sialylation is selectively enhanced, suggesting that an α2,3-sialyltransferase that catalyzes the sialyation of glycoproteins is activated or upregulated as the result of Rab11 knockdown.					
33524390	0	35	theme	sialyltransferase	43:59	arg1	ST3GAL4					61:67	the sialyltransferase ST3GAL4	39:67	the sialyltransferase ST3GAL4	39:67	Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.					
33524390	1	36	theme	each	324:327	arg1	monosaccharide					329:342	each monosaccharide	324:342	each monosaccharide	324:342	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	5	37	gly	sialylation	932:942	arg1	N-glycans					947:955	N-glycans	947:955	N-glycans	947:955	Our findings indicate that Rab11 knockdown results in a dramatic enhancement in the sialylation of N-glycans.					
33524390	8	38	from	distribution	1534:1545	arg1	puncta					1584:1589	other peripheral puncta	1567:1589	other peripheral puncta in control cells	1567:1606	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	8	38	from	distribution	1534:1545	arg1	Golgi					1554:1558	the Golgi	1550:1558	the Golgi	1550:1558	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	1	39	theme	monosaccharide	329:342	arg1	addition					312:319	the addition	308:319	the addition of each monosaccharide	308:342	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	2	40	theme	Golgi	438:442	arg1	apparatus					444:452	the Golgi apparatus	434:452	the Golgi apparatus	434:452	Glycosyltransferases primarily reside and function in the endoplasmic reticulum (ER) and the Golgi apparatus; whether and how they traffic beyond the Golgi, how this trafficking is controlled, and how it impacts glycosylation remain unclear.					
33524390	6	41	theme	Structural	958:967	arg1	analyses					969:976	Structural analyses	958:976	Structural analyses of glycans using lectins and LC-MS	958:1011	Structural analyses of glycans using lectins and LC-MS revealed that α2,3-sialylation is selectively enhanced, suggesting that an α2,3-sialyltransferase that catalyzes the sialyation of glycoproteins is activated or upregulated as the result of Rab11 knockdown.					
33524390	2	42	dep	whether	455:461	arg1	traffic					476:482	traffic	476:482	traffic	476:482	Glycosyltransferases primarily reside and function in the endoplasmic reticulum (ER) and the Golgi apparatus; whether and how they traffic beyond the Golgi, how this trafficking is controlled, and how it impacts glycosylation remain unclear.					
33524390	0	43	theme	ST3GAL4	61:67	arg1	transport					26:34	Rab11-mediated post-Golgi transport	0:34	Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4	0:67	Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.					
33524390	3	44	theme	post-Golgi	684:693	arg1	transport					695:703	the post-Golgi transport	680:703	the post-Golgi transport	680:703	Our previous work identified a connection between N-glycosylation and Rab11, a key player in the post-Golgi transport that connects recycling endosomes and other compartments.					
33524390	7	45	theme	substrate	1388:1396	arg1	levels					1350:1355	the levels	1346:1355	the levels of its mRNA, protein, or donor substrate	1346:1396	ST3GAL4 is the major α2,3-sialyltransferase that acts on N-glycans; we demonstrated that the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate, was altered by Rab11 depletion.					
33524390	7	46	theme	ST3GAL4	1329:1335	arg1	localization					1313:1324	the localization	1309:1324	the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate,	1309:1397	ST3GAL4 is the major α2,3-sialyltransferase that acts on N-glycans; we demonstrated that the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate, was altered by Rab11 depletion.					
33524390	8	47	theme	knockdown	1434:1442	arg1	cells					1444:1448	knockdown cells	1434:1448	knockdown cells	1434:1448	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	1	48	theme	tight	236:240	arg1	regulation					242:251	tight regulation	236:251	tight regulation of subcellular glycosyltransferase location	236:295	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	8	49	theme	trans-Golgi	1489:1499	arg1	network					1501:1507	the trans-Golgi network	1485:1507	the trans-Golgi network	1485:1507	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	4	50	theme	Rab11	804:808	arg1	role					796:799	the specific role	783:799	the specific role of Rab11	783:808	To learn more about the specific role of Rab11, we knocked down Rab11 in HeLa cells.					
33524390	1	51	theme	common	148:153	arg1	modification					173:184	the most common posttranslational modification	139:184	the most common posttranslational modification of proteins	139:196	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	1	51	theme	common	148:153	arg1	Glycosylation					124:136	Glycosylation	124:136	Glycosylation	124:136	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	9	52	theme	glycosyltransferase	1879:1897	arg1	regulation					1899:1908	glycosyltransferase regulation	1879:1908	glycosyltransferase regulation	1879:1908	This indicates that Rab11 may negatively regulate α2,3-sialylation by transporting ST3GAL4 to post-Golgi compartments (PGCs), which is a novel mechanism of glycosyltransferase regulation.					
33524390	8	53	theme	control	1594:1600	arg1	cells					1602:1606	control cells	1594:1606	control cells	1594:1606	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	0	54	theme	new	80:82	arg1	mechanism					84:92	a new mechanism	78:92	a new mechanism for regulating glycosylation	78:121	Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.					
33524390	1	55	theme	posttranslational	155:171	arg1	modification					173:184	the most common posttranslational modification	139:184	the most common posttranslational modification of proteins	139:196	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	1	55	theme	posttranslational	155:171	arg1	Glycosylation					124:136	Glycosylation	124:136	Glycosylation	124:136	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33524390	9	56	theme	regulation	1899:1908	arg1	mechanism					1866:1874	a novel mechanism	1858:1874	a novel mechanism of glycosyltransferase regulation	1858:1908	This indicates that Rab11 may negatively regulate α2,3-sialylation by transporting ST3GAL4 to post-Golgi compartments (PGCs), which is a novel mechanism of glycosyltransferase regulation.					
33524390	9	56	theme	regulation	1899:1908	arg1	compartments					1828:1839	post-Golgi compartments	1817:1839	post-Golgi compartments (PGCs)	1817:1846	This indicates that Rab11 may negatively regulate α2,3-sialylation by transporting ST3GAL4 to post-Golgi compartments (PGCs), which is a novel mechanism of glycosyltransferase regulation.					
33524390	8	57	theme	peripheral	1573:1582	arg1	puncta					1584:1589	other peripheral puncta	1567:1589	other peripheral puncta in control cells	1567:1606	In knockdown cells, ST3GAL4 is densely distributed in the trans-Golgi network, compared with the wider distribution in the Golgi and in other peripheral puncta in control cells, whereas the α2,6-sialyltransferase ST6GAL1 is predominantly localized to the Golgi regardless of Rab11 knockdown.					
33524390	3	58	theme	recycling	719:727	arg1	endosomes					729:737	recycling endosomes	719:737	recycling endosomes	719:737	Our previous work identified a connection between N-glycosylation and Rab11, a key player in the post-Golgi transport that connects recycling endosomes and other compartments.					
33524390	7	59	theme	protein	1370:1376	arg1	levels					1350:1355	the levels	1346:1355	the levels of its mRNA, protein, or donor substrate	1346:1396	ST3GAL4 is the major α2,3-sialyltransferase that acts on N-glycans; we demonstrated that the localization of ST3GAL4, but not the levels of its mRNA, protein, or donor substrate, was altered by Rab11 depletion.					
33524390	1	60	theme	subcellular	256:266	arg1	location					288:295	subcellular glycosyltransferase location	256:295	subcellular glycosyltransferase location	256:295	Glycosylation, the most common posttranslational modification of proteins, is a stepwise process that relies on tight regulation of subcellular glycosyltransferase location to control the addition of each monosaccharide.					
33566602	0	0	theme	Chicken	99:105	arg1	Membrane					121:128	Chicken Egg Vitelline Membrane	99:128	Chicken Egg Vitelline Membrane	99:128	Comparative N-Glycoproteomic Analysis Provides Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane during High-Temperature Storage.					
33566602	2	1	from	roles	434:438	arg1	deterioration					479:491	membrane deterioration	470:491	membrane deterioration	470:491	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	1	2	theme	chicken	180:186	arg1	CEVM					212:215	CEVM	212:215	CEVM	212:215	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	1	2	theme	chicken	180:186	arg1	membrane					202:209	chicken egg vitelline membrane	180:209	chicken egg vitelline membrane (CEVM)	180:216	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	5	3	from	Removal	839:845	arg1	Mucin-5B					867:874	Mucin-5B	867:874	Mucin-5B	867:874	Removal of the N-glycans in Mucin-5B may result in a loss of CEVM's mechanical properties.					
33566602	6	4	from	domain	983:988	arg1	CEVM					993:996	CEVM	993:996	CEVM	993:996	The N-glycosites enriched in the apolipoprotein B β2 domain in CEVM were significantly changed, which may contribute to lipid composition modifications during storage.					
33566602	5	5	theme	properties	918:927	arg1	loss					892:895	a loss	890:895	a loss of CEVM's mechanical properties	890:927	Removal of the N-glycans in Mucin-5B may result in a loss of CEVM's mechanical properties.					
33566602	1	6	theme	egg	188:190	arg1	CEVM					212:215	CEVM	212:215	CEVM	212:215	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	1	6	theme	egg	188:190	arg1	membrane					202:209	chicken egg vitelline membrane	180:209	chicken egg vitelline membrane (CEVM)	180:216	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	1	7	theme	egg	267:269	arg1	quality					271:277	egg quality	267:277	egg quality	267:277	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	0	8	theme	Vitelline	111:119	arg1	Membrane					121:128	Chicken Egg Vitelline Membrane	99:128	Chicken Egg Vitelline Membrane	99:128	Comparative N-Glycoproteomic Analysis Provides Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane during High-Temperature Storage.					
33566602	4	9	theme	aminopeptidase	814:827	arg1	activity					829:836	aminopeptidase activity	814:836	aminopeptidase activity	814:836	Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity.					
33566602	4	10	gly	N-glycosylated	696:709	arg1	DGPs					721:724	DGPs	721:724	DGPs	721:724	Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity.					
33566602	4	10	gly	N-glycosylated	696:709	arg1	proteins					711:718	differentially N-glycosylated proteins	681:718	these differentially N-glycosylated proteins (DGPs)	675:725	Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity.					
33566602	5	11	theme	mechanical	907:916	arg1	properties					918:927	CEVM's mechanical properties	900:927	CEVM's mechanical properties	900:927	Removal of the N-glycans in Mucin-5B may result in a loss of CEVM's mechanical properties.					
33566602	6	12	gly	N-glycosites	934:945	arg2	N-glycosites					934:945	The N-glycosites	930:945	The N-glycosites enriched in the apolipoprotein B β2 domain in CEVM	930:996	The N-glycosites enriched in the apolipoprotein B β2 domain in CEVM were significantly changed, which may contribute to lipid composition modifications during storage.					
33566602	0	13	theme	Egg	107:109	arg1	Membrane					121:128	Chicken Egg Vitelline Membrane	99:128	Chicken Egg Vitelline Membrane	99:128	Comparative N-Glycoproteomic Analysis Provides Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane during High-Temperature Storage.					
33566602	5	14	from	N-glycans	854:862	arg1	Mucin-5B					867:874	Mucin-5B	867:874	Mucin-5B	867:874	Removal of the N-glycans in Mucin-5B may result in a loss of CEVM's mechanical properties.					
33566602	4	15	theme	lipid	795:799	arg1	binding					801:807	lipid binding	795:807	lipid binding	795:807	Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity.					
33566602	7	16	from	N-glycosites	1108:1119	arg1	metalloproteases					1132:1147	several metalloproteases	1124:1147	several metalloproteases	1124:1147	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	4	17	theme	glycosaminoglycan	768:784	arg1	binding					786:792	glycosaminoglycan binding	768:792	glycosaminoglycan binding	768:792	Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity.					
33566602	8	18	theme	protein	1410:1416	arg1	N-glycosylation					1418:1432	protein N-glycosylation	1410:1432	protein N-glycosylation	1410:1432	These findings provide novel insights into the roles of protein N-glycosylation during membrane weakening.					
33566602	1	19	theme	vitelline	192:200	arg1	CEVM					212:215	CEVM	212:215	CEVM	212:215	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	1	19	theme	vitelline	192:200	arg1	membrane					202:209	chicken egg vitelline membrane	180:209	chicken egg vitelline membrane (CEVM)	180:216	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	7	20	theme	decreased	1234:1242	arg1	levels					1260:1265	the decreased N-glycosylation levels	1230:1265	the decreased N-glycosylation levels	1230:1265	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	7	21	theme	active	1194:1199	arg1	region					1206:1211	active site region	1194:1211	active site region	1194:1211	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	2	22	from	days	380:383	arg1	°C					402:403	30 °C	399:403	30 °C	399:403	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	0	23	theme	Comparative	0:10	arg1	Analysis					29:36	Comparative N-Glycoproteomic Analysis	0:36	Comparative N-Glycoproteomic Analysis	0:36	Comparative N-Glycoproteomic Analysis Provides Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane during High-Temperature Storage.					
33566602	1	24	theme	membrane	202:209	arg1	factors					247:253	the most important factors	228:253	the most important factors influencing egg quality during high-temperature storage	228:309	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	1	24	theme	membrane	202:209	arg1	one					221:223	one	221:223	one	221:223	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	1	24	theme	membrane	202:209	arg1	weakening					167:175	The weakening	163:175	The weakening of chicken egg vitelline membrane (CEVM)	163:216	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	0	25	theme	N-Glycoproteomic	12:27	arg1	Analysis					29:36	Comparative N-Glycoproteomic Analysis	0:36	Comparative N-Glycoproteomic Analysis	0:36	Comparative N-Glycoproteomic Analysis Provides Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane during High-Temperature Storage.					
33566602	2	26	theme	N-glycoproteomic	337:352	arg1	analysis					354:361	a comparative N-glycoproteomic analysis	323:361	a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C	323:403	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	2	27	theme	protein	443:449	arg1	N-glycosylation					451:465	protein N-glycosylation	443:465	protein N-glycosylation	443:465	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	1	28	theme	high-temperature	286:301	arg1	storage					303:309	high-temperature storage	286:309	high-temperature storage	286:309	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	7	29	theme	several	1124:1130	arg1	metalloproteases					1132:1147	several metalloproteases	1124:1147	several metalloproteases	1124:1147	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	2	30	theme	comparative	325:335	arg1	analysis					354:361	a comparative N-glycoproteomic analysis	323:361	a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C	323:403	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	4	31	theme	N-glycosylated	696:709	arg1	DGPs					721:724	DGPs	721:724	DGPs	721:724	Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity.					
33566602	4	31	theme	N-glycosylated	696:709	arg1	proteins					711:718	differentially N-glycosylated proteins	681:718	these differentially N-glycosylated proteins (DGPs)	675:725	Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity.					
33566602	5	32	theme	N-glycans	854:862	arg1	Removal					839:845	Removal	839:845	Removal of the N-glycans in Mucin-5B	839:874	Removal of the N-glycans in Mucin-5B may result in a loss of CEVM's mechanical properties.					
33566602	8	33	theme	N-glycosylation	1418:1432	arg1	roles					1401:1405	the roles	1397:1405	the roles of protein N-glycosylation during membrane weakening	1397:1458	These findings provide novel insights into the roles of protein N-glycosylation during membrane weakening.					
33566602	0	34	theme	High-Temperature	137:152	arg1	Storage					154:160	High-Temperature Storage	137:160	High-Temperature Storage	137:160	Comparative N-Glycoproteomic Analysis Provides Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane during High-Temperature Storage.					
33566602	8	35	theme	membrane	1441:1448	arg1	weakening					1450:1458	membrane weakening	1441:1458	membrane weakening	1441:1458	These findings provide novel insights into the roles of protein N-glycosylation during membrane weakening.					
33566602	3	36	from	proteins	601:608	arg1	N-glycosites					580:591	46 N-glycosites	577:591	46 N-glycosites from 30 proteins	577:608	In total, 399 N-glycosites corresponding to 198 proteins were identified, of which 46 N-glycosites from 30 proteins were significantly altered.					
33566602	4	37	theme	ontology	643:650	arg1	analysis					652:659	Gene ontology analysis	638:659	Gene ontology analysis	638:659	Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity.					
33566602	7	38	theme	N-glycosylation	1244:1258	arg1	levels					1260:1265	the decreased N-glycosylation levels	1230:1265	the decreased N-glycosylation levels	1230:1265	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	7	39	theme	enzyme	1337:1342	arg1	activity					1344:1351	enzyme activity	1337:1351	enzyme activity	1337:1351	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	7	40	theme	specific	1306:1313	arg1	binding					1325:1331	specific substrate binding	1306:1331	specific substrate binding	1306:1331	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	8	41	theme	novel	1377:1381	arg1	insights					1383:1390	novel insights	1377:1390	novel insights into the roles of protein N-glycosylation during membrane weakening	1377:1458	These findings provide novel insights into the roles of protein N-glycosylation during membrane weakening.					
33566602	0	42	theme	Novel	47:51	arg1	Insights					53:60	Novel Insights	47:60	Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane	47:128	Comparative N-Glycoproteomic Analysis Provides Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane during High-Temperature Storage.					
33566602	6	43	theme	β2	980:981	arg1	domain					983:988	the apolipoprotein B β2 domain	959:988	the apolipoprotein B β2 domain in CEVM	959:996	The N-glycosites enriched in the apolipoprotein B β2 domain in CEVM were significantly changed, which may contribute to lipid composition modifications during storage.					
33566602	7	44	theme	site	1201:1204	arg1	region					1206:1211	active site region	1194:1211	active site region	1194:1211	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	0	45	from	Mechanisms	85:94	arg1	Membrane					121:128	Chicken Egg Vitelline Membrane	99:128	Chicken Egg Vitelline Membrane	99:128	Comparative N-Glycoproteomic Analysis Provides Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane during High-Temperature Storage.					
33566602	4	46	theme	Gene	638:641	arg1	analysis					652:659	Gene ontology analysis	638:659	Gene ontology analysis	638:659	Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity.					
33566602	6	47	theme	composition	1056:1066	arg1	modifications					1068:1080	lipid composition modifications	1050:1080	lipid composition modifications during storage	1050:1095	The N-glycosites enriched in the apolipoprotein B β2 domain in CEVM were significantly changed, which may contribute to lipid composition modifications during storage.					
33566602	2	48	theme	membrane	470:477	arg1	deterioration					479:491	membrane deterioration	470:491	membrane deterioration	470:491	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	6	49	theme	B	978:978	arg1	domain					983:988	the apolipoprotein B β2 domain	959:988	the apolipoprotein B β2 domain in CEVM	959:996	The N-glycosites enriched in the apolipoprotein B β2 domain in CEVM were significantly changed, which may contribute to lipid composition modifications during storage.					
33566602	7	50	theme	structural	1284:1293	arg1	stability					1295:1303	their structural stability	1278:1303	their structural stability	1278:1303	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	3	51	gly	N-glycosites	580:591	arg2	N-glycosites					580:591	46 N-glycosites	577:591	46 N-glycosites from 30 proteins	577:608	In total, 399 N-glycosites corresponding to 198 proteins were identified, of which 46 N-glycosites from 30 proteins were significantly altered.					
33566602	7	52	gly	N-glycosites	1108:1119	arg2	N-glycosites					1108:1119	N-glycosites	1108:1119	N-glycosites in several metalloproteases	1108:1147	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	6	53	theme	apolipoprotein	963:976	arg1	domain					983:988	the apolipoprotein B β2 domain	959:988	the apolipoprotein B β2 domain in CEVM	959:996	The N-glycosites enriched in the apolipoprotein B β2 domain in CEVM were significantly changed, which may contribute to lipid composition modifications during storage.					
33566602	7	54	theme	functional	1173:1182	arg1	domain					1184:1189	the functional domain	1169:1189	the functional domain	1169:1189	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	5	55	from	Mucin-5B	867:874	arg1	Removal					839:845	Removal	839:845	Removal of the N-glycans in Mucin-5B	839:874	Removal of the N-glycans in Mucin-5B may result in a loss of CEVM's mechanical properties.					
33566602	2	56	theme	CEVM	366:369	arg1	analysis					354:361	a comparative N-glycoproteomic analysis	323:361	a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C	323:403	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	7	57	theme	substrate	1315:1323	arg1	binding					1325:1331	specific substrate binding	1306:1331	specific substrate binding	1306:1331	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	2	58	from	°C	402:403	arg1	days					380:383	10 days	377:383	10 days of storage at 30 °C	377:403	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	2	58	from	°C	402:403	arg1	storage					388:394	storage	388:394	storage at 30 °C	388:403	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	6	59	theme	lipid	1050:1054	arg1	modifications					1068:1080	lipid composition modifications	1050:1080	lipid composition modifications during storage	1050:1095	The N-glycosites enriched in the apolipoprotein B β2 domain in CEVM were significantly changed, which may contribute to lipid composition modifications during storage.					
33566602	3	60	gly	N-glycosites	508:519	arg2	N-glycosites					508:519	399 N-glycosites	504:519	399 N-glycosites corresponding to 198 proteins	504:549	In total, 399 N-glycosites corresponding to 198 proteins were identified, of which 46 N-glycosites from 30 proteins were significantly altered.					
33566602	2	61	gly	N-glycosylation	451:465	arg1	membrane					470:477	membrane deterioration	470:491	membrane deterioration	470:491	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	4	62	theme	antibacterial	744:756	arg1	activity					758:765	antibacterial activity	744:765	antibacterial activity	744:765	Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity.					
33566602	7	63	located	located	1154:1160	arg2	N-glycosites					1108:1119	N-glycosites	1108:1119	N-glycosites in several metalloproteases	1108:1147	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	7	63	located	located	1154:1160	arg1	domain					1184:1189	the functional domain	1169:1189	the functional domain	1169:1189	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	7	63	located	located	1154:1160	arg1	region					1206:1211	active site region	1194:1211	active site region	1194:1211	Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity.					
33566602	2	64	theme	storage	388:394	arg1	days					380:383	10 days	377:383	10 days of storage at 30 °C	377:403	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	1	65	theme	important	237:245	arg1	factors					247:253	the most important factors	228:253	the most important factors influencing egg quality during high-temperature storage	228:309	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	0	66	theme	Deterioration	71:83	arg1	Mechanisms					85:94	the Deterioration Mechanisms	67:94	the Deterioration Mechanisms in Chicken Egg Vitelline Membrane	67:128	Comparative N-Glycoproteomic Analysis Provides Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane during High-Temperature Storage.					
33566602	2	67	theme	N-glycosylation	451:465	arg1	roles					434:438	the roles	430:438	the roles of protein N-glycosylation in membrane deterioration	430:491	Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration.					
33566602	1	68	theme	factors	247:253	arg1	factors					247:253	the most important factors	228:253	the most important factors influencing egg quality during high-temperature storage	228:309	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	1	68	theme	factors	247:253	arg1	one					221:223	one	221:223	one	221:223	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33566602	1	68	theme	factors	247:253	arg1	weakening					167:175	The weakening	163:175	The weakening of chicken egg vitelline membrane (CEVM)	163:216	The weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage.					
33877489	11	0	theme	disease	1531:1537	arg1	character					1514:1522	the heterogenous character	1497:1522	the heterogenous character of the disease	1497:1537	It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers.					
33877489	5	1	theme	cancer	594:599	arg1	detection					574:582	detection	574:582	detection of breast cancer	574:599	In this study, the biomarker potential of total serum N-glycosylation analysis was explored with regard to detection of breast cancer.					
33877489	9	2	located	found	1124:1128	arg1	comparison					1089:1098	a case-control comparison	1074:1098	a case-control comparison	1074:1098	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	9	2	located	found	1124:1128	arg2	associations					1106:1117	three associations	1100:1117	three associations	1100:1117	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	6	3	from	patients	694:701	arg1	profiles					662:669	serum protein N-glycan profiles	639:669	serum protein N-glycan profiles from 145 breast cancer patients	639:701	In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals.					
33877489	12	4	theme	serum	1669:1673	arg1	profiles					1684:1691	serum N-glycan profiles	1669:1691	serum N-glycan profiles	1669:1691	It was found that serum N-glycan profiles differed for the various cancer subtypes that were analyzed in this study.					
33877489	12	5	theme	various	1710:1716	arg1	subtypes					1725:1732	the various cancer subtypes	1706:1732	the various cancer subtypes that were analyzed in this study	1706:1765	It was found that serum N-glycan profiles differed for the various cancer subtypes that were analyzed in this study.					
33877489	9	6	theme	triantennary	1161:1172	arg1	glycans					1174:1180	a two triantennary glycans	1155:1180	a two triantennary glycans	1155:1180	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	9	7	theme	glycans	1174:1180	arg1	level					1146:1150	a lower level	1138:1150	a lower level of a two triantennary glycans	1138:1180	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	9	7	theme	glycans	1174:1180	arg1	level					1195:1199	a higher level	1186:1199	a higher level of one tetraantennary glycan in cancer patients	1186:1247	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	7	8	theme	sialic	853:858	arg1	acids					860:864	sialic acids	853:864	sialic acids	853:864	N-glycans were enzymatically released, chemically derivatized to preserve linkage-specificity of sialic acids and characterized by high resolution mass spectrometry.					
33877489	8	9	theme	N-glycosylation	1028:1042	arg1	traits					1044:1049	N-glycosylation traits	1028:1049	specific N-glycan structures as well as N-glycosylation traits with breast cancer	988:1068	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
33877489	6	10	theme	healthy	735:741	arg1	individuals					743:753	171 healthy individuals	731:753	171 healthy individuals	731:753	In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals.					
33877489	11	11	theme	different	1632:1640	arg1	numbers					1642:1648	different numbers	1632:1648	different numbers	1632:1648	It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers.					
33877489	7	12	theme	acids	860:864	arg1	linkage-specificity					830:848	linkage-specificity	830:848	linkage-specificity of sialic acids	830:864	N-glycans were enzymatically released, chemically derivatized to preserve linkage-specificity of sialic acids and characterized by high resolution mass spectrometry.					
33877489	11	13	dep	cancer	1439:1444	arg1	signatures					1457:1466	N-glycomic signatures	1446:1466	breast cancer N-glycomic signatures	1432:1466	It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers.					
33877489	8	14	theme	traits	1044:1049	arg1	associations					972:983	associations	972:983	associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer	972:1068	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
33877489	8	15	theme	specific	988:995	arg1	structures					1006:1015	specific N-glycan structures	988:1015	specific N-glycan structures as well as N-glycosylation traits with breast cancer	988:1068	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
33877489	11	16	theme	cancer	1439:1444	arg1	replication					1417:1427	replication	1417:1427	replication of breast cancer N-glycomic signatures	1417:1466	It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers.					
33877489	10	17	theme	various	1259:1265	arg1	signatures					1284:1293	various other N-glycomic signatures	1259:1293	various other N-glycomic signatures that had previously been reported	1259:1327	Of note, various other N-glycomic signatures that had previously been reported were not replicated in the current cohort.					
33877489	3	18	theme	screening	319:327	arg1	tests					352:356	blood tests	346:356	blood tests	346:356	However, the sensitivity of this screening modality is not optimal and new screening methods, such as blood tests, are being explored.					
33877489	3	18	theme	screening	319:327	arg1	methods					329:335	new screening methods	315:335	new screening methods	315:335	However, the sensitivity of this screening modality is not optimal and new screening methods, such as blood tests, are being explored.					
33877489	8	19	theme	N-glycan	997:1004	arg1	structures					1006:1015	specific N-glycan structures	988:1015	specific N-glycan structures as well as N-glycosylation traits with breast cancer	988:1068	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
33877489	4	20	from	proteins	438:445	arg1	bloodstream					454:464	the bloodstream	450:464	the bloodstream	450:464	Most of the analyses that aim for early detection focus on proteins in the bloodstream.					
33877489	9	21	theme	higher	1188:1193	arg1	level					1195:1199	a higher level	1186:1199	a higher level of one tetraantennary glycan in cancer patients	1186:1247	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	1	22	theme	Breast	67:72	arg1	cancer					103:108	the most prevalent cancer	84:108	the most prevalent cancer in women	84:117	Breast cancer is the most prevalent cancer in women.					
33877489	1	22	theme	Breast	67:72	arg1	cancer					74:79	Breast cancer	67:79	Breast cancer	67:79	Breast cancer is the most prevalent cancer in women.					
33877489	8	23	with	traits	1044:1049	arg1	cancer					1063:1068	breast cancer	1056:1068	breast cancer	1056:1068	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
33877489	8	24	theme	breast	1056:1061	arg1	cancer					1063:1068	breast cancer	1056:1068	breast cancer	1056:1068	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
33877489	5	25	theme	total	509:513	arg1	analysis					537:544	total serum N-glycosylation analysis	509:544	total serum N-glycosylation analysis	509:544	In this study, the biomarker potential of total serum N-glycosylation analysis was explored with regard to detection of breast cancer.					
33877489	6	26	theme	N-glycan	653:660	arg1	profiles					662:669	serum protein N-glycan profiles	639:669	serum protein N-glycan profiles from 145 breast cancer patients	639:701	In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals.					
33877489	8	27	with	structures	1006:1015	arg1	cancer					1063:1068	breast cancer	1056:1068	breast cancer	1056:1068	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
33877489	0	28	theme	N-glycan	6:13	arg1	profiles					15:22	Serum N-glycan profiles	0:22	Serum N-glycan profiles	0:22	Serum N-glycan profiles differ for various breast cancer subtypes.					
33877489	2	29	theme	Early	120:124	arg1	detection					126:134	Early detection	120:134	Early detection of this disease	120:150	Early detection of this disease improves survival and therefore population screenings, based on mammography, are performed.					
33877489	5	30	theme	serum	515:519	arg1	analysis					537:544	total serum N-glycosylation analysis	509:544	total serum N-glycosylation analysis	509:544	In this study, the biomarker potential of total serum N-glycosylation analysis was explored with regard to detection of breast cancer.					
33877489	6	31	theme	protein	645:651	arg1	profiles					662:669	serum protein N-glycan profiles	639:669	serum protein N-glycan profiles from 145 breast cancer patients	639:701	In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals.					
33877489	0	32	theme	Serum	0:4	arg1	profiles					15:22	Serum N-glycan profiles	0:22	Serum N-glycan profiles	0:22	Serum N-glycan profiles differ for various breast cancer subtypes.					
33877489	11	33	theme	N-glycomic	1446:1455	arg1	signatures					1457:1466	N-glycomic signatures	1446:1466	breast cancer N-glycomic signatures	1432:1466	It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers.					
33877489	5	34	theme	breast	587:592	arg1	cancer					594:599	breast cancer	587:599	breast cancer	587:599	In this study, the biomarker potential of total serum N-glycosylation analysis was explored with regard to detection of breast cancer.					
33877489	1	35	from	cancer	103:108	arg1	women					113:117	women	113:117	women	113:117	Breast cancer is the most prevalent cancer in women.					
33877489	6	36	theme	serum	639:643	arg1	profiles					662:669	serum protein N-glycan profiles	639:669	serum protein N-glycan profiles from 145 breast cancer patients	639:701	In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals.					
33877489	11	37	from	subtypes	1620:1627	arg1	numbers					1642:1648	different numbers	1632:1648	different numbers	1632:1648	It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers.					
33877489	5	38	theme	N-glycosylation	521:535	arg1	analysis					537:544	total serum N-glycosylation analysis	509:544	total serum N-glycosylation analysis	509:544	In this study, the biomarker potential of total serum N-glycosylation analysis was explored with regard to detection of breast cancer.					
33877489	5	39	theme	analysis	537:544	arg1	potential					496:504	the biomarker potential	482:504	the biomarker potential of total serum N-glycosylation analysis	482:544	In this study, the biomarker potential of total serum N-glycosylation analysis was explored with regard to detection of breast cancer.					
33877489	3	40	theme	blood	346:350	arg1	tests					352:356	blood tests	346:356	blood tests	346:356	However, the sensitivity of this screening modality is not optimal and new screening methods, such as blood tests, are being explored.					
33877489	9	41	theme	lower	1140:1144	arg1	level					1146:1150	a lower level	1138:1150	a lower level of a two triantennary glycans	1138:1180	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	9	42	theme	glycan	1223:1228	arg1	level					1146:1150	a lower level	1138:1150	a lower level of a two triantennary glycans	1138:1180	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	9	42	theme	glycan	1223:1228	arg1	level					1195:1199	a higher level	1186:1199	a higher level of one tetraantennary glycan in cancer patients	1186:1247	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	1	43	theme	prevalent	93:101	arg1	cancer					103:108	the most prevalent cancer	84:108	the most prevalent cancer in women	84:117	Breast cancer is the most prevalent cancer in women.					
33877489	1	43	theme	prevalent	93:101	arg1	cancer					74:79	Breast cancer	67:79	Breast cancer	67:79	Breast cancer is the most prevalent cancer in women.					
33877489	6	44	theme	case-control	620:631	arg1	setup					633:637	an age-matched case-control setup	605:637	an age-matched case-control setup	605:637	In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals.					
33877489	9	45	from	level	1146:1150	arg1	patients					1240:1247	cancer patients	1233:1247	cancer patients	1233:1247	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	0	46	theme	various	35:41	arg1	subtypes					57:64	various breast cancer subtypes	35:64	various breast cancer subtypes	35:64	Serum N-glycan profiles differ for various breast cancer subtypes.					
33877489	9	47	theme	case-control	1076:1087	arg1	comparison					1089:1098	a case-control comparison	1074:1098	a case-control comparison	1074:1098	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	6	48	theme	age-matched	608:618	arg1	setup					633:637	an age-matched case-control setup	605:637	an age-matched case-control setup	605:637	In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals.					
33877489	11	49	theme	breast	1432:1437	arg1	cancer					1439:1444	breast cancer N-glycomic signatures	1432:1466	breast cancer N-glycomic signatures	1432:1466	It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers.					
33877489	12	50	theme	N-glycan	1675:1682	arg1	profiles					1684:1691	serum N-glycan profiles	1669:1691	serum N-glycan profiles	1669:1691	It was found that serum N-glycan profiles differed for the various cancer subtypes that were analyzed in this study.					
33877489	4	51	theme	early	413:417	arg1	detection					419:427	early detection	413:427	early detection	413:427	Most of the analyses that aim for early detection focus on proteins in the bloodstream.					
33877489	0	52	theme	cancer	50:55	arg1	subtypes					57:64	various breast cancer subtypes	35:64	various breast cancer subtypes	35:64	Serum N-glycan profiles differ for various breast cancer subtypes.					
33877489	3	53	theme	modality	287:294	arg1	sensitivity					257:267	the sensitivity	253:267	the sensitivity of this screening modality	253:294	However, the sensitivity of this screening modality is not optimal and new screening methods, such as blood tests, are being explored.					
33877489	3	53	theme	modality	287:294	arg1	optimal					303:309	optimal	303:309	optimal	303:309	However, the sensitivity of this screening modality is not optimal and new screening methods, such as blood tests, are being explored.					
33877489	2	54	theme	disease	144:150	arg1	detection					126:134	Early detection	120:134	Early detection of this disease	120:150	Early detection of this disease improves survival and therefore population screenings, based on mammography, are performed.					
33877489	5	55	theme	biomarker	486:494	arg1	potential					496:504	the biomarker potential	482:504	the biomarker potential of total serum N-glycosylation analysis	482:544	In this study, the biomarker potential of total serum N-glycosylation analysis was explored with regard to detection of breast cancer.					
33877489	0	56	theme	breast	43:48	arg1	subtypes					57:64	various breast cancer subtypes	35:64	various breast cancer subtypes	35:64	Serum N-glycan profiles differ for various breast cancer subtypes.					
33877489	11	57	theme	replication	1417:1427	arg1	lack					1409:1412	the lack	1405:1412	the lack of replication of breast cancer N-glycomic signatures	1405:1466	It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers.					
33877489	10	58	theme	N-glycomic	1273:1282	arg1	signatures					1284:1293	various other N-glycomic signatures	1259:1293	various other N-glycomic signatures that had previously been reported	1259:1327	Of note, various other N-glycomic signatures that had previously been reported were not replicated in the current cohort.					
33877489	8	59	theme	regression	931:940	arg1	analysis					942:949	Logistic regression analysis	922:949	Logistic regression analysis	922:949	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
33877489	9	60	theme	cancer	1233:1238	arg1	patients					1240:1247	cancer patients	1233:1247	cancer patients	1233:1247	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	7	61	theme	resolution	892:901	arg1	spectrometry					908:919	high resolution mass spectrometry	887:919	high resolution mass spectrometry	887:919	N-glycans were enzymatically released, chemically derivatized to preserve linkage-specificity of sialic acids and characterized by high resolution mass spectrometry.					
33877489	9	62	theme	tetraantennary	1208:1221	arg1	glycan					1223:1228	one tetraantennary glycan	1204:1228	one tetraantennary glycan	1204:1228	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	8	63	theme	structures	1006:1015	arg1	associations					972:983	associations	972:983	associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer	972:1068	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
33877489	8	64	theme	Logistic	922:929	arg1	analysis					942:949	Logistic regression analysis	922:949	Logistic regression analysis	922:949	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
33877489	9	65	from	level	1195:1199	arg1	patients					1240:1247	cancer patients	1233:1247	cancer patients	1233:1247	In a case-control comparison three associations were found, namely a lower level of a two triantennary glycans and a higher level of one tetraantennary glycan in cancer patients.					
33877489	7	66	theme	mass	903:906	arg1	spectrometry					908:919	high resolution mass spectrometry	887:919	high resolution mass spectrometry	887:919	N-glycans were enzymatically released, chemically derivatized to preserve linkage-specificity of sialic acids and characterized by high resolution mass spectrometry.					
33877489	3	67	theme	screening	277:285	arg1	modality					287:294	this screening modality	272:294	this screening modality	272:294	However, the sensitivity of this screening modality is not optimal and new screening methods, such as blood tests, are being explored.					
33877489	2	68	theme	population	184:193	arg1	screenings					195:204	population screenings	184:204	population screenings	184:204	Early detection of this disease improves survival and therefore population screenings, based on mammography, are performed.					
33877489	11	69	theme	diverging	1610:1618	arg1	subtypes					1620:1627	diverging subtypes	1610:1627	diverging subtypes in different numbers	1610:1648	It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers.					
33877489	12	70	theme	cancer	1718:1723	arg1	subtypes					1725:1732	the various cancer subtypes	1706:1732	the various cancer subtypes that were analyzed in this study	1706:1765	It was found that serum N-glycan profiles differed for the various cancer subtypes that were analyzed in this study.					
33877489	10	71	theme	other	1267:1271	arg1	signatures					1284:1293	various other N-glycomic signatures	1259:1293	various other N-glycomic signatures that had previously been reported	1259:1327	Of note, various other N-glycomic signatures that had previously been reported were not replicated in the current cohort.					
33877489	10	72	theme	current	1356:1362	arg1	cohort					1364:1369	the current cohort	1352:1369	the current cohort	1352:1369	Of note, various other N-glycomic signatures that had previously been reported were not replicated in the current cohort.					
33877489	11	73	theme	heterogenous	1501:1512	arg1	character					1514:1522	the heterogenous character	1497:1522	the heterogenous character of the disease	1497:1537	It was further evaluated whether the lack of replication of breast cancer N-glycomic signatures could be partly explained by the heterogenous character of the disease since the studies performed so far were based on cohorts that included diverging subtypes in different numbers.					
33877489	6	74	theme	cancer	687:692	arg1	patients					694:701	145 breast cancer patients	676:701	145 breast cancer patients	676:701	In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals.					
33877489	7	75	theme	high	887:890	arg1	spectrometry					908:919	high resolution mass spectrometry	887:919	high resolution mass spectrometry	887:919	N-glycans were enzymatically released, chemically derivatized to preserve linkage-specificity of sialic acids and characterized by high resolution mass spectrometry.					
33877489	3	76	theme	new	315:317	arg1	tests					352:356	blood tests	346:356	blood tests	346:356	However, the sensitivity of this screening modality is not optimal and new screening methods, such as blood tests, are being explored.					
33877489	3	76	theme	new	315:317	arg1	methods					329:335	new screening methods	315:335	new screening methods	315:335	However, the sensitivity of this screening modality is not optimal and new screening methods, such as blood tests, are being explored.					
33877489	6	77	theme	breast	680:685	arg1	patients					694:701	145 breast cancer patients	676:701	145 breast cancer patients	676:701	In an age-matched case-control setup serum protein N-glycan profiles from 145 breast cancer patients were compared to those from 171 healthy individuals.					
33877489	8	78	used	used	955:958	arg2	analysis					942:949	Logistic regression analysis	922:949	Logistic regression analysis	922:949	Logistic regression analysis was used to evaluate associations of specific N-glycan structures as well as N-glycosylation traits with breast cancer.					
32973324	7	0	theme	coli	1057:1060	arg1	O55					1062:1064	E. coli O55	1054:1064	E. coli O55	1054:1064	In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination of E. coli O55.					
32973324	9	1	theme	attachment	1288:1297	arg1	inhibition					1274:1283	the species-specific inhibition	1253:1283	the species-specific inhibition of attachment to corresponding epithelial cells	1253:1331	Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.					
32973324	1	2	theme	diverse	146:152	arg1	microbiota					164:173	highly diverse commensal microbiota	139:173	highly diverse commensal microbiota	139:173	Mucosal surfaces are colonized by highly diverse commensal microbiota.					
32973324	5	3	theme	secretory	696:704	arg1	Fc-SC					717:721	Fc-SC	717:721	Fc-SC	717:721	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	5	3	theme	secretory	696:704	arg1	component					706:714	the secretory component	692:714	the secretory component (Fc-SC)	692:722	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	4	4	dep	studies	457:463	arg1	contrast					431:438	contrast	431:438	contrast	431:438	In contrast to many in vitro studies, only a few reported the effect of SIgA glycans in vivo.					
32973324	0	5	theme	O55	69:71	arg1	infection					73:81	E. coli O55 infection	61:81	E. coli O55 infection of germ-free piglets	61:102	Secretory IgA N-glycans contribute to the protection against E. coli O55 infection of germ-free piglets.					
32973324	7	6	theme	E.	1054:1055	arg1	O55					1062:1064	E. coli O55	1054:1064	E. coli O55	1054:1064	In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination of E. coli O55.					
32973324	5	7	theme	SIgA	620:623	arg1	effect					610:615	the protective effect	595:615	the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC)	595:722	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	5	8	theme	newborn	562:568	arg1	piglets					570:576	antibody-free newborn piglets	548:576	a germ-free antibody-free newborn piglets model	536:582	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	1	9	theme	commensal	154:162	arg1	microbiota					164:173	highly diverse commensal microbiota	139:173	highly diverse commensal microbiota	139:173	Mucosal surfaces are colonized by highly diverse commensal microbiota.					
32973324	6	10	theme	clinical	934:941	arg1	status					943:948	clinical status	934:948	clinical status	934:948	SIgA, Fab, and Fc-SC were protective, whereas removal of N-glycans from SIgA reduced SIgA-mediated protection as demonstrated by piglets' intestinal histology, clinical status, and survival.					
32973324	0	11	theme	germ-free	86:94	arg1	piglets					96:102	germ-free piglets	86:102	germ-free piglets	86:102	Secretory IgA N-glycans contribute to the protection against E. coli O55 infection of germ-free piglets.					
32973324	6	12	theme	N-glycans	831:839	arg1	removal					820:826	removal	820:826	removal of N-glycans from SIgA	820:849	SIgA, Fab, and Fc-SC were protective, whereas removal of N-glycans from SIgA reduced SIgA-mediated protection as demonstrated by piglets' intestinal histology, clinical status, and survival.					
32973324	8	13	theme	N-glycans	1120:1128	arg1	role					1096:1099	the role	1092:1099	the role of SIgA-associated N-glycans in protection	1092:1142	These findings highlight the role of SIgA-associated N-glycans in protection.					
32973324	5	14	theme	piglets	570:576	arg1	model					578:582	a germ-free antibody-free newborn piglets model	536:582	a germ-free antibody-free newborn piglets model	536:582	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	4	15	dep	in	448:449	arg1	vitro					451:455	vitro	451:455	vitro	451:455	In contrast to many in vitro studies, only a few reported the effect of SIgA glycans in vivo.					
32973324	5	16	with	Fab	669:671	arg1	N-glycans					658:666	enzymatically removed N-glycans	636:666	enzymatically removed N-glycans	636:666	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	5	17	theme	SIgA	626:629	arg1	effect					610:615	the protective effect	595:615	the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC)	595:722	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	7	18	gly	deglycosylation	998:1012	arg1	SIgA					1017:1020	SIgA	1017:1020	SIgA	1017:1020	In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination of E. coli O55.					
32973324	8	19	theme	SIgA-associated	1104:1118	arg1	N-glycans					1120:1128	SIgA-associated N-glycans	1104:1128	SIgA-associated N-glycans	1104:1128	These findings highlight the role of SIgA-associated N-glycans in protection.					
32973324	7	20	dep	In	965:966	arg1	vitro					968:972	vitro	968:972	vitro	968:972	In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination of E. coli O55.					
32973324	0	21	theme	piglets	96:102	arg1	infection					73:81	E. coli O55 infection	61:81	E. coli O55 infection of germ-free piglets	61:102	Secretory IgA N-glycans contribute to the protection against E. coli O55 infection of germ-free piglets.					
32973324	5	22	contain	containing	681:690	arg1	SIgA					620:623	SIgA	620:623	SIgA	620:623	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	5	22	contain	containing	681:690	arg2	Fc-SC					717:721	Fc-SC	717:721	Fc-SC	717:721	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	5	22	contain	containing	681:690	arg2	component					706:714	the secretory component	692:714	the secretory component (Fc-SC)	692:722	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	7	23	theme	SIgA	1017:1020	arg1	deglycosylation					998:1012	deglycosylation	998:1012	deglycosylation of SIgA	998:1020	In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination of E. coli O55.					
32973324	5	24	theme	O55	759:761	arg1	challenge					763:771	oral necrotoxigenic E. coli O55 challenge	731:771	oral necrotoxigenic E. coli O55 challenge	731:771	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	0	25	theme	IgA	10:12	arg1	N-glycans					14:22	Secretory IgA N-glycans	0:22	Secretory IgA N-glycans	0:22	Secretory IgA N-glycans contribute to the protection against E. coli O55 infection of germ-free piglets.					
32973324	6	26	from	SIgA	846:849	arg1	removal					820:826	removal	820:826	removal of N-glycans from SIgA	820:849	SIgA, Fab, and Fc-SC were protective, whereas removal of N-glycans from SIgA reduced SIgA-mediated protection as demonstrated by piglets' intestinal histology, clinical status, and survival.					
32973324	5	27	theme	protective	599:608	arg1	effect					610:615	the protective effect	595:615	the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC)	595:722	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	9	28	theme	structural	1153:1162	arg1	studies					1164:1170	Further structural studies	1145:1170	Further structural studies of SIgA-associated glycans	1145:1197	Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.					
32973324	9	29	theme	species-specific	1257:1272	arg1	inhibition					1274:1283	the species-specific inhibition	1253:1283	the species-specific inhibition of attachment to corresponding epithelial cells	1253:1331	Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.					
32973324	5	30	used	used	531:534	arg2	we					528:529	we	528:529	we	528:529	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	0	31	theme	Secretory	0:8	arg1	N-glycans					14:22	Secretory IgA N-glycans	0:22	Secretory IgA N-glycans	0:22	Secretory IgA N-glycans contribute to the protection against E. coli O55 infection of germ-free piglets.					
32973324	6	32	theme	intestinal	912:921	arg1	histology					923:931	piglets' intestinal histology	903:931	piglets' intestinal histology	903:931	SIgA, Fab, and Fc-SC were protective, whereas removal of N-glycans from SIgA reduced SIgA-mediated protection as demonstrated by piglets' intestinal histology, clinical status, and survival.					
32973324	5	33	with	SIgA	626:629	arg1	N-glycans					658:666	enzymatically removed N-glycans	636:666	enzymatically removed N-glycans	636:666	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	5	34	theme	germ-free	538:546	arg1	model					578:582	a germ-free antibody-free newborn piglets model	536:582	a germ-free antibody-free newborn piglets model	536:582	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	5	35	theme	Fc	678:679	arg1	effect					610:615	the protective effect	595:615	the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC)	595:722	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	7	36	theme	O55	1062:1064	arg1	agglutination					1037:1049	agglutination	1037:1049	agglutination of E. coli O55	1037:1064	In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination of E. coli O55.					
32973324	9	37	theme	corresponding	1302:1314	arg1	cells					1327:1331	corresponding epithelial cells	1302:1331	corresponding epithelial cells	1302:1331	Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.					
32973324	5	38	theme	antibody-free	548:560	arg1	piglets					570:576	antibody-free newborn piglets	548:576	a germ-free antibody-free newborn piglets model	536:582	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	9	39	theme	glycans	1191:1197	arg1	studies					1164:1170	Further structural studies	1145:1170	Further structural studies of SIgA-associated glycans	1145:1197	Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.					
32973324	9	40	theme	epithelial	1316:1325	arg1	cells					1327:1331	corresponding epithelial cells	1302:1331	corresponding epithelial cells	1302:1331	Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.					
32973324	5	41	theme	Fab	669:671	arg1	effect					610:615	the protective effect	595:615	the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC)	595:722	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	4	42	theme	in	448:449	arg1	studies					457:463	many in vitro studies	443:463	many in vitro studies	443:463	In contrast to many in vitro studies, only a few reported the effect of SIgA glycans in vivo.					
32973324	0	43	dep	E.	61:62	arg1	coli					64:67	coli	64:67	coli	64:67	Secretory IgA N-glycans contribute to the protection against E. coli O55 infection of germ-free piglets.					
32973324	3	44	theme	SIgA-associated	403:417	arg1	glycans					419:425	the SIgA-associated glycans	399:425	the SIgA-associated glycans	399:425	Bacterial coating could be mediated by antigen-specific SIgA recognition, polyreactivity, and/or by the SIgA-associated glycans.					
32973324	4	45	theme	many	443:446	arg1	studies					457:463	many in vitro studies	443:463	many in vitro studies	443:463	In contrast to many in vitro studies, only a few reported the effect of SIgA glycans in vivo.					
32973324	5	46	theme	oral	731:734	arg1	challenge					763:771	oral necrotoxigenic E. coli O55 challenge	731:771	oral necrotoxigenic E. coli O55 challenge	731:771	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	4	47	theme	glycans	505:511	arg1	effect					490:495	the effect	486:495	the effect of SIgA glycans	486:511	In contrast to many in vitro studies, only a few reported the effect of SIgA glycans in vivo.					
32973324	3	48	theme	antigen-specific	338:353	arg1	polyreactivity					373:386	polyreactivity	373:386	polyreactivity	373:386	Bacterial coating could be mediated by antigen-specific SIgA recognition, polyreactivity, and/or by the SIgA-associated glycans.					
32973324	3	48	theme	antigen-specific	338:353	arg1	recognition					360:370	antigen-specific SIgA recognition	338:370	antigen-specific SIgA recognition	338:370	Bacterial coating could be mediated by antigen-specific SIgA recognition, polyreactivity, and/or by the SIgA-associated glycans.					
32973324	5	49	theme	necrotoxigenic	736:749	arg1	challenge					763:771	oral necrotoxigenic E. coli O55 challenge	731:771	oral necrotoxigenic E. coli O55 challenge	731:771	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	3	50	theme	SIgA	355:358	arg1	polyreactivity					373:386	polyreactivity	373:386	polyreactivity	373:386	Bacterial coating could be mediated by antigen-specific SIgA recognition, polyreactivity, and/or by the SIgA-associated glycans.					
32973324	3	50	theme	SIgA	355:358	arg1	recognition					360:370	antigen-specific SIgA recognition	338:370	antigen-specific SIgA recognition	338:370	Bacterial coating could be mediated by antigen-specific SIgA recognition, polyreactivity, and/or by the SIgA-associated glycans.					
32973324	5	51	with	Fc	678:679	arg1	N-glycans					658:666	enzymatically removed N-glycans	636:666	enzymatically removed N-glycans	636:666	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	7	52	theme	In	965:966	arg1	analyses					974:981	In vitro analyses	965:981	In vitro analyses	965:981	In vitro analyses indicated that deglycosylation of SIgA did not reduce agglutination of E. coli O55.					
32973324	1	53	theme	Mucosal	105:111	arg1	surfaces					113:120	Mucosal surfaces	105:120	Mucosal surfaces	105:120	Mucosal surfaces are colonized by highly diverse commensal microbiota.					
32973324	5	54	theme	E.	751:752	arg1	challenge					763:771	oral necrotoxigenic E. coli O55 challenge	731:771	oral necrotoxigenic E. coli O55 challenge	731:771	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	3	55	theme	Bacterial	299:307	arg1	coating					309:315	Bacterial coating	299:315	Bacterial coating	299:315	Bacterial coating could be mediated by antigen-specific SIgA recognition, polyreactivity, and/or by the SIgA-associated glycans.					
32973324	6	56	theme	SIgA-mediated	859:871	arg1	protection					873:882	SIgA-mediated protection	859:882	SIgA-mediated protection	859:882	SIgA, Fab, and Fc-SC were protective, whereas removal of N-glycans from SIgA reduced SIgA-mediated protection as demonstrated by piglets' intestinal histology, clinical status, and survival.					
32973324	5	57	theme	removed	650:656	arg1	N-glycans					658:666	enzymatically removed N-glycans	636:666	enzymatically removed N-glycans	636:666	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	9	58	theme	Further	1145:1151	arg1	studies					1164:1170	Further structural studies	1145:1170	Further structural studies of SIgA-associated glycans	1145:1197	Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.					
32973324	9	59	theme	SIgA-associated	1175:1189	arg1	glycans					1191:1197	SIgA-associated glycans	1175:1197	SIgA-associated glycans	1175:1197	Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.					
32973324	5	60	with	SIgA	620:623	arg1	N-glycans					658:666	enzymatically removed N-glycans	636:666	enzymatically removed N-glycans	636:666	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	8	61	from	role	1096:1099	arg1	protection					1133:1142	protection	1133:1142	protection	1133:1142	These findings highlight the role of SIgA-associated N-glycans in protection.					
32973324	2	62	theme	bacteria	245:252	arg1	survival					223:230	the survival	219:230	the survival of commensal bacteria	219:252	Coating with secretory IgA (SIgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens.					
32973324	2	63	with	Coating	176:182	arg1	IgA					199:201	secretory IgA	189:201	secretory IgA (SIgA)	189:208	Coating with secretory IgA (SIgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens.					
32973324	2	63	with	Coating	176:182	arg1	SIgA					204:207	SIgA	204:207	SIgA	204:207	Coating with secretory IgA (SIgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens.					
32973324	2	64	theme	secretory	189:197	arg1	IgA					199:201	secretory IgA	189:201	secretory IgA (SIgA)	189:208	Coating with secretory IgA (SIgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens.					
32973324	2	64	theme	secretory	189:197	arg1	SIgA					204:207	SIgA	204:207	SIgA	204:207	Coating with secretory IgA (SIgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens.					
32973324	2	65	theme	commensal	235:243	arg1	bacteria					245:252	commensal bacteria	235:252	commensal bacteria	235:252	Coating with secretory IgA (SIgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens.					
32973324	0	66	theme	E.	61:62	arg1	infection					73:81	E. coli O55 infection	61:81	E. coli O55 infection of germ-free piglets	61:102	Secretory IgA N-glycans contribute to the protection against E. coli O55 infection of germ-free piglets.					
32973324	9	67	theme	those	1235:1239	arg1	identification					1217:1230	the identification	1213:1230	the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells	1213:1331	Further structural studies of SIgA-associated glycans would lead to the identification of those involved in the species-specific inhibition of attachment to corresponding epithelial cells.					
32973324	5	68	theme	coli	754:757	arg1	challenge					763:771	oral necrotoxigenic E. coli O55 challenge	731:771	oral necrotoxigenic E. coli O55 challenge	731:771	Here, we used a germ-free antibody-free newborn piglets model to compare the protective effect of SIgA, SIgA with enzymatically removed N-glycans, Fab, and Fc containing the secretory component (Fc-SC) during oral necrotoxigenic E. coli O55 challenge.					
32973324	4	69	theme	SIgA	500:503	arg1	glycans					505:511	SIgA glycans	500:511	SIgA glycans	500:511	In contrast to many in vitro studies, only a few reported the effect of SIgA glycans in vivo.					
31967297	6	0	theme	processing	1087:1096	arg1	differences					1098:1108	clear processing differences	1081:1108	clear processing differences	1081:1108	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	2	1	theme	-linked	319:325	arg1	glycans					327:333	five asparagine (N)-linked glycans	300:333	five asparagine (N)-linked glycans	300:333	The extracellular domain is decorated with five asparagine (N)-linked glycans; N-glycans at N162 and N45 directly contribute to high-affinity antibody binding and protein stability.					
31967297	4	2	from	variations	657:666	arg1	structures					682:691	N45 glycan structures	671:691	N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors	671:815	In this study, we identified variations in N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors.					
31967297	1	3	theme	monocyte	229:236	arg1	function					247:254	monocyte effector function	229:254	monocyte effector function	229:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	3	4	theme	CD16a	533:537	arg1	study					524:528	a recent study	515:528	a recent study of CD16a isolated from primary human NK cells	515:574	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	0	5	theme	Allotype-specific	0:16	arg1	processing					18:27	Allotype-specific processing	0:27	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.	0:106	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.					
31967297	9	6	theme	monocytes	1457:1465	arg1	pool					1449:1452	a pool	1447:1452	a pool of monocytes	1447:1465	CD16a H48 purified from a pool of monocytes similarly displayed increased processing at N45.					
31967297	3	7	theme	human	561:565	arg1	cells					570:574	primary human NK cells	553:574	primary human NK cells	553:574	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	1	8	theme	well-defined	179:190	arg1	role					192:195	a well-defined role	177:195	a well-defined role in natural killer (NK) cell and monocyte effector function	177:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	6	9	theme	protein	955:961	arg1	analysis					943:950	A mass spectrometry analysis	923:950	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants	923:1066	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	10	10	theme	primary	1598:1604	arg1	cells					1609:1613	primary NK cells	1598:1613	primary NK cells	1598:1613	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	6	11	theme	clear	1081:1085	arg1	differences					1098:1108	clear processing differences	1081:1108	clear processing differences	1081:1108	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	5	12	theme	recurrent	895:903	arg1	infections					911:920	recurrent viral infections	895:920	recurrent viral infections	895:920	It is known that H48 homozygous individuals suffer from immunodeficiency and recurrent viral infections.					
31967297	10	13	theme	healthy	1634:1640	arg1	donors					1648:1653	healthy human donors	1634:1653	healthy human donors	1634:1653	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	1	14	theme	killer	208:213	arg1	cell					220:223	natural killer (NK) cell	200:223	natural killer (NK) cell	200:223	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	6	15	theme	mass	925:928	arg1	analysis					943:950	A mass spectrometry analysis	923:950	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants	923:1066	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	8	16	theme	N-glycosylation	1299:1313	arg1	sites					1315:1319	the four other N-glycosylation sites	1284:1319	the four other N-glycosylation sites	1284:1319	Structures at the four other N-glycosylation sites showed minimal differences from data collected on donors expressing only the predominant L48 variant.					
31967297	1	17	from	role	192:195	arg1	cell					220:223	natural killer (NK) cell	200:223	natural killer (NK) cell	200:223	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	1	17	from	role	192:195	arg1	function					247:254	monocyte effector function	229:254	monocyte effector function	229:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	1	18	theme	receptor	113:120	arg1	IIIa/CD16a					122:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	1	18	theme	receptor	113:120	arg1	receptor					163:170	an activating cell surface receptor	136:170	an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function	136:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	6	19	attach	isolated	963:970	arg1	cells					1004:1008	the primary natural killer cells	977:1008	the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants	977:1066	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	6	19	attach	isolated	963:970	arg2	protein					955:961	protein	955:961	protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants	955:1066	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	7	20	with	allotype	1219:1226	arg1	N45-glycoforms					1254:1267	predominantly hybrid N45-glycoforms	1233:1267	predominantly hybrid N45-glycoforms	1233:1267	CD16a H48 displayed a greater proportion of complex-type N45 glycans compared to the more common L48 allotype with predominantly hybrid N45-glycoforms.					
31967297	3	21	theme	N-glycan	439:446	arg1	structures					448:457	N-glycan structures	439:457	N-glycan structures at N162	439:465	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	0	22	theme	human	66:70	arg1	cells					87:91	primary human natural killer cells	58:91	primary human natural killer cells	58:91	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.					
31967297	4	23	from	position	767:774	arg1	leucine					756:762	leucine	756:762	histidine instead of leucine at position 48 of CD16a from two heterozygous donors	735:815	In this study, we identified variations in N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors.					
31967297	1	24	theme	activating	139:148	arg1	IIIa/CD16a					122:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	1	24	theme	activating	139:148	arg1	receptor					163:170	an activating cell surface receptor	136:170	an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function	136:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	6	25	theme	killer	997:1002	arg1	cells					1004:1008	the primary natural killer cells	977:1008	the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants	977:1066	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	4	26	theme	N45	671:673	arg1	structures					682:691	N45 glycan structures	671:691	N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors	671:815	In this study, we identified variations in N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors.					
31967297	0	27	theme	killer	80:85	arg1	cells					87:91	primary human natural killer cells	58:91	primary human natural killer cells	58:91	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.					
31967297	1	28	theme	surface	155:161	arg1	IIIa/CD16a					122:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	1	28	theme	surface	155:161	arg1	receptor					163:170	an activating cell surface receptor	136:170	an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function	136:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	6	29	theme	primary	981:987	arg1	cells					1004:1008	the primary natural killer cells	977:1008	the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants	977:1066	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	2	30	theme	extracellular	261:273	arg1	domain					275:280	The extracellular domain	257:280	The extracellular domain	257:280	The extracellular domain is decorated with five asparagine (N)-linked glycans; N-glycans at N162 and N45 directly contribute to high-affinity antibody binding and protein stability.					
31967297	0	31	theme	N45-glycan	42:51	arg1	processing					18:27	Allotype-specific processing	0:27	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.	0:106	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.					
31967297	8	32	from	data	1353:1356	arg1	differences					1336:1346	minimal differences	1328:1346	minimal differences from data collected on donors expressing only the predominant L48 variant	1328:1420	Structures at the four other N-glycosylation sites showed minimal differences from data collected on donors expressing only the predominant L48 variant.					
31967297	2	33	theme	protein	420:426	arg1	stability					428:436	protein stability	420:436	protein stability	420:436	The extracellular domain is decorated with five asparagine (N)-linked glycans; N-glycans at N162 and N45 directly contribute to high-affinity antibody binding and protein stability.					
31967297	7	34	theme	glycans	1179:1185	arg1	proportion					1148:1157	a greater proportion	1138:1157	a greater proportion of complex-type N45 glycans	1138:1185	CD16a H48 displayed a greater proportion of complex-type N45 glycans compared to the more common L48 allotype with predominantly hybrid N45-glycoforms.					
31967297	8	35	theme	L48	1410:1412	arg1	variant					1414:1420	only the predominant L48 variant	1389:1420	only the predominant L48 variant	1389:1420	Structures at the four other N-glycosylation sites showed minimal differences from data collected on donors expressing only the predominant L48 variant.					
31967297	2	36	theme	high-affinity	385:397	arg1	binding					408:414	high-affinity antibody binding	385:414	high-affinity antibody binding	385:414	The extracellular domain is decorated with five asparagine (N)-linked glycans; N-glycans at N162 and N45 directly contribute to high-affinity antibody binding and protein stability.					
31967297	7	37	theme	complex-type	1162:1173	arg1	glycans					1179:1185	complex-type N45 glycans	1162:1185	complex-type N45 glycans	1162:1185	CD16a H48 displayed a greater proportion of complex-type N45 glycans compared to the more common L48 allotype with predominantly hybrid N45-glycoforms.					
31967297	1	38	theme	Fc	108:109	arg1	IIIa/CD16a					122:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	1	38	theme	Fc	108:109	arg1	receptor					163:170	an activating cell surface receptor	136:170	an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function	136:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	6	39	theme	CD16a	1041:1045	arg1	L48					1047:1049	CD16a L48	1041:1049	CD16a L48	1041:1049	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	0	40	theme	CD16a	36:40	arg1	N45-glycan					42:51	the CD16a N45-glycan	32:51	the CD16a N45-glycan	32:51	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.					
31967297	2	41	from	N162	349:352	arg1	N-glycans					336:344	N-glycans	336:344	N-glycans at N162 and N45	336:360	The extracellular domain is decorated with five asparagine (N)-linked glycans; N-glycans at N162 and N45 directly contribute to high-affinity antibody binding and protein stability.					
31967297	10	42	theme	CD16a	1547:1551	arg1	processing					1553:1562	CD16a processing	1547:1562	CD16a processing	1547:1562	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	4	43	theme	heterozygous	797:808	arg1	donors					810:815	two heterozygous donors	793:815	two heterozygous donors	793:815	In this study, we identified variations in N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors.					
31967297	2	44	from	N45	358:360	arg1	N-glycans					336:344	N-glycans	336:344	N-glycans at N162 and N45	336:360	The extracellular domain is decorated with five asparagine (N)-linked glycans; N-glycans at N162 and N45 directly contribute to high-affinity antibody binding and protein stability.					
31967297	9	45	theme	CD16a	1423:1427	arg1	H48					1429:1431	CD16a H48	1423:1431	CD16a H48 purified from a pool of monocytes	1423:1465	CD16a H48 purified from a pool of monocytes similarly displayed increased processing at N45.					
31967297	7	46	theme	CD16a	1118:1122	arg1	H48					1124:1126	CD16a H48	1118:1126	CD16a H48	1118:1126	CD16a H48 displayed a greater proportion of complex-type N45 glycans compared to the more common L48 allotype with predominantly hybrid N45-glycoforms.					
31967297	3	47	theme	donor-dependent	486:500	arg1	variation					502:510	significant donor-dependent variation	474:510	significant donor-dependent variation	474:510	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	7	48	theme	hybrid	1247:1252	arg1	N45-glycoforms					1254:1267	predominantly hybrid N45-glycoforms	1233:1267	predominantly hybrid N45-glycoforms	1233:1267	CD16a H48 displayed a greater proportion of complex-type N45 glycans compared to the more common L48 allotype with predominantly hybrid N45-glycoforms.					
31967297	1	49	theme	effector	238:245	arg1	function					247:254	monocyte effector function	229:254	monocyte effector function	229:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	10	50	theme	H48	1583:1585	arg1	residue					1587:1593	the H48 residue	1579:1593	the H48 residue in primary NK cells and monocytes from healthy human donors	1579:1653	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	2	51	link	-linked	319:325	arg1	glycans					327:333	five asparagine (N)-linked glycans	300:333	five asparagine (N)-linked glycans	300:333	The extracellular domain is decorated with five asparagine (N)-linked glycans; N-glycans at N162 and N45 directly contribute to high-affinity antibody binding and protein stability.					
31967297	3	52	theme	recent	517:522	arg1	study					524:528	a recent study	515:528	a recent study of CD16a isolated from primary human NK cells	515:574	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	7	53	theme	L48	1215:1217	arg1	allotype					1219:1226	the more common L48 allotype	1199:1226	the more common L48 allotype with predominantly hybrid N45-glycoforms	1199:1267	CD16a H48 displayed a greater proportion of complex-type N45 glycans compared to the more common L48 allotype with predominantly hybrid N45-glycoforms.					
31967297	3	54	from	N162	462:465	arg1	structures					448:457	N-glycan structures	439:457	N-glycan structures at N162	439:465	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	3	55	theme	primary	553:559	arg1	cells					570:574	primary human NK cells	553:574	primary human NK cells	553:574	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	10	56	theme	NK	1606:1607	arg1	cells					1609:1613	primary NK cells	1598:1613	primary NK cells	1598:1613	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	9	57	theme	increased	1487:1495	arg1	processing					1497:1506	increased processing	1487:1506	increased processing	1487:1506	CD16a H48 purified from a pool of monocytes similarly displayed increased processing at N45.					
31967297	0	58	from	cells	87:91	arg1	processing					18:27	Allotype-specific processing	0:27	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.	0:106	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.					
31967297	10	59	theme	human	1642:1646	arg1	donors					1648:1653	healthy human donors	1634:1653	healthy human donors	1634:1653	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	5	60	theme	homozygous	839:848	arg1	individuals					850:860	H48 homozygous individuals	835:860	H48 homozygous individuals	835:860	It is known that H48 homozygous individuals suffer from immunodeficiency and recurrent viral infections.					
31967297	1	61	theme	natural	200:206	arg1	NK					216:217	NK	216:217	NK	216:217	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	1	61	theme	natural	200:206	arg1	killer					208:213	natural killer	200:213	natural killer (NK) cell	200:223	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	6	62	theme	spectrometry	930:941	arg1	analysis					943:950	A mass spectrometry analysis	923:950	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants	923:1066	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	3	63	theme	NK	567:568	arg1	cells					570:574	primary human NK cells	553:574	primary human NK cells	553:574	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	1	64	theme	γ	111:111	arg1	IIIa/CD16a					122:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	1	64	theme	γ	111:111	arg1	receptor					163:170	an activating cell surface receptor	136:170	an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function	136:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	8	65	theme	other	1293:1297	arg1	sites					1315:1319	the four other N-glycosylation sites	1284:1319	the four other N-glycosylation sites	1284:1319	Structures at the four other N-glycosylation sites showed minimal differences from data collected on donors expressing only the predominant L48 variant.					
31967297	5	66	theme	viral	905:909	arg1	infections					911:920	recurrent viral infections	895:920	recurrent viral infections	895:920	It is known that H48 homozygous individuals suffer from immunodeficiency and recurrent viral infections.					
31967297	6	67	theme	individuals	1013:1023	arg1	cells					1004:1008	the primary natural killer cells	977:1008	the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants	977:1066	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	0	68	from	monocytes	97:105	arg1	processing					18:27	Allotype-specific processing	0:27	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.	0:106	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.					
31967297	0	69	theme	natural	72:78	arg1	cells					87:91	primary human natural killer cells	58:91	primary human natural killer cells	58:91	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.					
31967297	8	70	theme	minimal	1328:1334	arg1	differences					1336:1346	minimal differences	1328:1346	minimal differences from data collected on donors expressing only the predominant L48 variant	1328:1420	Structures at the four other N-glycosylation sites showed minimal differences from data collected on donors expressing only the predominant L48 variant.					
31967297	7	71	theme	common	1208:1213	arg1	allotype					1219:1226	the more common L48 allotype	1199:1226	the more common L48 allotype with predominantly hybrid N45-glycoforms	1199:1267	CD16a H48 displayed a greater proportion of complex-type N45 glycans compared to the more common L48 allotype with predominantly hybrid N45-glycoforms.					
31967297	8	72	gly	N-glycosylation	1299:1313	arg2	four					1288:1291	four	1288:1291	four	1288:1291	Structures at the four other N-glycosylation sites showed minimal differences from data collected on donors expressing only the predominant L48 variant.					
31967297	8	72	gly	N-glycosylation	1299:1313	arg2	sites					1315:1319	the four other N-glycosylation sites	1284:1319	the four other N-glycosylation sites	1284:1319	Structures at the four other N-glycosylation sites showed minimal differences from data collected on donors expressing only the predominant L48 variant.					
31967297	1	73	theme	cell	150:153	arg1	IIIa/CD16a					122:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a	108:131	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	1	73	theme	cell	150:153	arg1	receptor					163:170	an activating cell surface receptor	136:170	an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function	136:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	6	74	theme	natural	989:995	arg1	cells					1004:1008	the primary natural killer cells	977:1008	the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants	977:1066	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	8	75	from	sites	1315:1319	arg1	Structures					1270:1279	Structures	1270:1279	Structures at the four other N-glycosylation sites	1270:1319	Structures at the four other N-glycosylation sites showed minimal differences from data collected on donors expressing only the predominant L48 variant.					
31967297	3	76	from	N45	595:597	arg1	structures					581:590	structures	581:590	structures at N45	581:597	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	7	77	theme	N45	1175:1177	arg1	glycans					1179:1185	complex-type N45 glycans	1162:1185	complex-type N45 glycans	1162:1185	CD16a H48 displayed a greater proportion of complex-type N45 glycans compared to the more common L48 allotype with predominantly hybrid N45-glycoforms.					
31967297	4	78	from	donors	810:815	arg1	position					767:774	position 48	767:777	position 48 of CD16a from two heterozygous donors	767:815	In this study, we identified variations in N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors.					
31967297	3	79	attach	isolated	539:546	arg2	CD16a					533:537	CD16a	533:537	CD16a isolated from primary human NK cells	533:574	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	3	79	attach	isolated	539:546	arg1	cells					570:574	primary human NK cells	553:574	primary human NK cells	553:574	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	6	80	theme	H48	1055:1057	arg1	variants					1059:1066	H48 variants	1055:1066	H48 variants	1055:1066	A mass spectrometry analysis of protein isolated from the primary natural killer cells of individuals expressing both CD16a L48 and H48 variants demonstrated clear processing differences at N45.					
31967297	2	81	theme	antibody	399:406	arg1	binding					408:414	high-affinity antibody binding	385:414	high-affinity antibody binding	385:414	The extracellular domain is decorated with five asparagine (N)-linked glycans; N-glycans at N162 and N45 directly contribute to high-affinity antibody binding and protein stability.					
31967297	8	82	theme	predominant	1398:1408	arg1	variant					1414:1420	only the predominant L48 variant	1389:1420	only the predominant L48 variant	1389:1420	Structures at the four other N-glycosylation sites showed minimal differences from data collected on donors expressing only the predominant L48 variant.					
31967297	7	83	theme	greater	1140:1146	arg1	proportion					1148:1157	a greater proportion	1138:1157	a greater proportion of complex-type N45 glycans	1138:1185	CD16a H48 displayed a greater proportion of complex-type N45 glycans compared to the more common L48 allotype with predominantly hybrid N45-glycoforms.					
31967297	4	84	theme	CD16a	782:786	arg1	position					767:774	position 48	767:777	position 48 of CD16a from two heterozygous donors	767:815	In this study, we identified variations in N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors.					
31967297	0	85	theme	primary	58:64	arg1	cells					87:91	primary human natural killer cells	58:91	primary human natural killer cells	58:91	Allotype-specific processing of the CD16a N45-glycan from primary human natural killer cells and monocytes.					
31967297	10	86	from	donors	1648:1653	arg1	cells					1609:1613	primary NK cells	1598:1613	primary NK cells	1598:1613	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	10	86	from	donors	1648:1653	arg1	residue					1587:1593	the H48 residue	1579:1593	the H48 residue in primary NK cells and monocytes from healthy human donors	1579:1653	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	10	86	from	donors	1648:1653	arg1	monocytes					1619:1627	monocytes	1619:1627	monocytes	1619:1627	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	3	87	theme	significant	474:484	arg1	variation					502:510	significant donor-dependent variation	474:510	significant donor-dependent variation	474:510	N-glycan structures at N162 showed significant donor-dependent variation in a recent study of CD16a isolated from primary human NK cells, but structures at N45 were relatively homogeneous.					
31967297	1	88	with	receptor	163:170	arg1	role					192:195	a well-defined role	177:195	a well-defined role in natural killer (NK) cell and monocyte effector function	177:254	Fc γ receptor IIIa/CD16a is an activating cell surface receptor with a well-defined role in natural killer (NK) cell and monocyte effector function.					
31967297	10	89	from	residue	1587:1593	arg1	cells					1609:1613	primary NK cells	1598:1613	primary NK cells	1598:1613	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	10	89	from	residue	1587:1593	arg1	donors					1648:1653	healthy human donors	1634:1653	healthy human donors	1634:1653	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	10	89	from	residue	1587:1593	arg1	monocytes					1619:1627	monocytes	1619:1627	monocytes	1619:1627	Here, we provide evidence that CD16a processing is affected by the H48 residue in primary NK cells and monocytes from healthy human donors.					
31967297	4	90	theme	glycan	675:680	arg1	structures					682:691	N45 glycan structures	671:691	N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors	671:815	In this study, we identified variations in N45 glycan structures associated with a polymorphism coding for histidine instead of leucine at position 48 of CD16a from two heterozygous donors.					
33954318	0	0	theme	di-fucosyl-β-cyclodextrin	80:104	arg1	properties					52:61	Digestion, fermentation, and pathogen anti-adhesive properties	0:61	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin	0:104	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin.					
33954318	9	1	theme	fucosylated	1565:1575	arg1	glycans					1577:1583	fucosylated glycans	1565:1583	fucosylated glycans	1565:1583	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	5	2	theme	anti-adhesive	857:869	arg1	capacity					871:878	anti-adhesive capacity	857:878	anti-adhesive capacity	857:878	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	0	3	theme	hMO-mimic	70:78	arg1	di-fucosyl-β-cyclodextrin					80:104	the hMO-mimic di-fucosyl-β-cyclodextrin	66:104	the hMO-mimic di-fucosyl-β-cyclodextrin	66:104	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin.					
33954318	9	4	theme	glycans	1577:1583	arg1	power					1556:1560	the anti-adhesive power	1538:1560	the anti-adhesive power of fucosylated glycans	1538:1583	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	2	5	theme	oligosaccharides	321:336	arg1	set					303:305	a large set	295:305	a large set of human milk oligosaccharides (hMOs)	295:343	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	2	5	theme	oligosaccharides	321:336	arg1	hMOs					339:342	hMOs	339:342	hMOs	339:342	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	2	5	theme	oligosaccharides	321:336	arg1	oligosaccharides					321:336	human milk oligosaccharides	310:336	human milk oligosaccharides (hMOs)	310:343	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	9	6	theme	biological	1440:1449	arg1	evaluation					1451:1460	biological evaluation	1440:1460	biological evaluation	1440:1460	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	2	7	theme	molecules	274:282	arg1	plethora					252:259	a plethora	250:259	a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs)	250:343	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	2	8	contain	contains	241:248	arg2	plethora					252:259	a plethora	250:259	a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs)	250:343	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	2	8	contain	contains	241:248	arg1	milk					231:234	Human milk	225:234	Human milk	225:234	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	5	9	theme	di-fucosyl-β-cyclodextrin	902:926	arg1	fermentation					840:851	fermentation	840:851	fermentation	840:851	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	5	9	theme	di-fucosyl-β-cyclodextrin	902:926	arg1	capacity					871:878	anti-adhesive capacity	857:878	anti-adhesive capacity	857:878	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	5	9	theme	di-fucosyl-β-cyclodextrin	902:926	arg1	digestion					829:837	digestion	829:837	digestion	829:837	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	9	10	theme	fucosylated	1471:1481	arg1	hMOs					1483:1486	other fucosylated hMOs	1465:1486	other fucosylated hMOs	1465:1486	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	2	11	theme	bioactive	264:272	arg1	set					303:305	a large set	295:305	a large set of human milk oligosaccharides (hMOs)	295:343	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	2	11	theme	bioactive	264:272	arg1	molecules					274:282	bioactive molecules	264:282	bioactive molecules	264:282	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	2	11	theme	bioactive	264:272	arg1	oligosaccharides					321:336	human milk oligosaccharides	310:336	human milk oligosaccharides (hMOs)	310:343	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	6	12	from	inoculum	1128:1135	arg1	enzymes					1094:1100	microbial enzymes	1084:1100	microbial enzymes from a 9 month-old infant inoculum	1084:1135	We establish that DFβCD is not digested by α-amylase and also resistant to fermentation by microbial enzymes from a 9 month-old infant inoculum.					
33954318	4	13	theme	so-called	685:693	arg1	"					702:702	so-called "mimics"	685:702	so-called "mimics"	685:702	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	4	13	theme	so-called	685:693	arg1	structures					673:682	hMO structures	669:682	hMO structures (so-called "mimics")	669:703	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	9	14	theme	hMOs	1483:1486	arg1	generation					1425:1434	generation	1425:1434	generation	1425:1434	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	9	14	theme	hMOs	1483:1486	arg1	evaluation					1451:1460	biological evaluation	1440:1460	biological evaluation	1440:1460	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	3	15	gly	fucosylated	357:367	arg1	hMOs					369:372	Especially fucosylated hMOs	346:372	Especially fucosylated hMOs	346:372	Especially fucosylated hMOs have received attention for their anti-adhesive effects on pathogens, preventing attachment to the intestine and infection.					
33954318	6	16	theme	infant	1121:1126	arg1	inoculum					1128:1135	a 9 month-old infant inoculum	1107:1135	a 9 month-old infant inoculum	1107:1135	We establish that DFβCD is not digested by α-amylase and also resistant to fermentation by microbial enzymes from a 9 month-old infant inoculum.					
33954318	4	17	theme	novel	632:636	arg1	compounds					638:646	novel compounds	632:646	novel compounds that are inspired by hMO structures (so-called "mimics")	632:703	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	4	17	theme	novel	632:636	arg1	compounds					721:729	interesting compounds	709:729	interesting compounds to produce and evaluate for their biological effects	709:782	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	3	18	theme	fucosylated	357:367	arg1	hMOs					369:372	Especially fucosylated hMOs	346:372	Especially fucosylated hMOs	346:372	Especially fucosylated hMOs have received attention for their anti-adhesive effects on pathogens, preventing attachment to the intestine and infection.					
33954318	6	19	theme	month-old	1111:1119	arg1	inoculum					1128:1135	a 9 month-old infant inoculum	1107:1135	a 9 month-old infant inoculum	1107:1135	We establish that DFβCD is not digested by α-amylase and also resistant to fermentation by microbial enzymes from a 9 month-old infant inoculum.					
33954318	7	20	theme	Caco-2	1225:1230	arg1	cells					1232:1236	Caco-2 cells	1225:1236	Caco-2 cells	1225:1236	In addition, we reveal that DFβCD blocks adhesion of enterotoxigenic E. coli (ETEC) to Caco-2 cells, especially when DFβCD is pre-incubated with ETEC prior to addition to the Caco-2 cells.					
33954318	2	21	theme	large	297:301	arg1	set					303:305	a large set	295:305	a large set of human milk oligosaccharides (hMOs)	295:343	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	2	21	theme	large	297:301	arg1	hMOs					339:342	hMOs	339:342	hMOs	339:342	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	2	21	theme	large	297:301	arg1	oligosaccharides					321:336	human milk oligosaccharides	310:336	human milk oligosaccharides (hMOs)	310:343	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	0	22	theme	Digestion	0:8	arg1	properties					52:61	Digestion, fermentation, and pathogen anti-adhesive properties	0:61	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin	0:104	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin.					
33954318	9	23	gly	fucosylated	1471:1481	arg1	hMOs					1483:1486	other fucosylated hMOs	1465:1486	other fucosylated hMOs	1465:1486	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	9	24	theme	anti-adhesive	1542:1554	arg1	power					1556:1560	the anti-adhesive power	1538:1560	the anti-adhesive power of fucosylated glycans	1538:1583	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	1	25	theme	Human	107:111	arg1	food					153:156	the best food	144:156	the best food for infants	144:168	Human milk is widely acknowledged as the best food for infants, and that is not just because of nutritional features.					
33954318	1	25	theme	Human	107:111	arg1	milk					113:116	Human milk	107:116	Human milk	107:116	Human milk is widely acknowledged as the best food for infants, and that is not just because of nutritional features.					
33954318	3	26	theme	anti-adhesive	408:420	arg1	effects					422:428	their anti-adhesive effects	402:428	their anti-adhesive effects on pathogens	402:441	Especially fucosylated hMOs have received attention for their anti-adhesive effects on pathogens, preventing attachment to the intestine and infection.					
33954318	5	27	theme	molecular	963:971	arg1	structures					973:982	the molecular structures	959:982	the molecular structures of hMOs	959:990	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	4	28	theme	interesting	709:719	arg1	compounds					638:646	novel compounds	632:646	novel compounds that are inspired by hMO structures (so-called "mimics")	632:703	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	4	28	theme	interesting	709:719	arg1	compounds					721:729	interesting compounds	709:729	interesting compounds to produce and evaluate for their biological effects	709:782	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	5	29	dep	digestion	829:837	arg1	the					825:827	the	825:827	the	825:827	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	4	30	theme	infant	616:621	arg1	formula					623:629	(medical) infant formula	606:629	(medical) infant formula	606:629	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	0	31	theme	fermentation	11:22	arg1	properties					52:61	Digestion, fermentation, and pathogen anti-adhesive properties	0:61	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin	0:104	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin.					
33954318	8	32	theme	decoy	1372:1376	arg1	effect					1378:1383	a decoy effect	1370:1383	a decoy effect	1370:1383	This suggests that DFβCD functions through a decoy effect.					
33954318	4	33	theme	hMO	669:671	arg1	"					702:702	so-called "mimics"	685:702	so-called "mimics"	685:702	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	4	33	theme	hMO	669:671	arg1	structures					673:682	hMO structures	669:682	hMO structures (so-called "mimics")	669:703	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	0	34	theme	pathogen	29:36	arg1	properties					52:61	Digestion, fermentation, and pathogen anti-adhesive properties	0:61	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin	0:104	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin.					
33954318	4	35	theme	sufficient	551:560	arg1	quantities					562:571	sufficient quantities	551:571	sufficient quantities to study	551:580	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	6	36	theme	microbial	1084:1092	arg1	enzymes					1094:1100	microbial enzymes	1084:1100	microbial enzymes from a 9 month-old infant inoculum	1084:1135	We establish that DFβCD is not digested by α-amylase and also resistant to fermentation by microbial enzymes from a 9 month-old infant inoculum.					
33954318	2	37	theme	Human	225:229	arg1	milk					231:234	Human milk	225:234	Human milk	225:234	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	5	38	theme	thorough	805:812	arg1	study					814:818	our thorough study	801:818	our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs	801:990	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	9	39	theme	power	1556:1560	arg1	overview					1526:1533	a comprehensive overview	1510:1533	a comprehensive overview of the anti-adhesive power of fucosylated glycans	1510:1583	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	5	40	theme	hMOs	987:990	arg1	structures					973:982	the molecular structures	959:982	the molecular structures of hMOs	959:990	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	9	41	theme	mimics	1492:1497	arg1	generation					1425:1434	generation	1425:1434	generation	1425:1434	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	9	41	theme	mimics	1492:1497	arg1	evaluation					1451:1460	biological evaluation	1440:1460	biological evaluation	1440:1460	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	9	42	theme	comprehensive	1512:1524	arg1	overview					1526:1533	a comprehensive overview	1510:1533	a comprehensive overview of the anti-adhesive power of fucosylated glycans	1510:1583	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	2	43	theme	milk	316:319	arg1	hMOs					339:342	hMOs	339:342	hMOs	339:342	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	2	43	theme	milk	316:319	arg1	oligosaccharides					321:336	human milk oligosaccharides	310:336	human milk oligosaccharides (hMOs)	310:343	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	0	44	theme	anti-adhesive	38:50	arg1	properties					52:61	Digestion, fermentation, and pathogen anti-adhesive properties	0:61	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin	0:104	Digestion, fermentation, and pathogen anti-adhesive properties of the hMO-mimic di-fucosyl-β-cyclodextrin.					
33954318	9	45	dep	generation	1425:1434	arg1	the					1421:1423	the	1421:1423	the	1421:1423	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	2	46	theme	human	310:314	arg1	hMOs					339:342	hMOs	339:342	hMOs	339:342	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	2	46	theme	human	310:314	arg1	oligosaccharides					321:336	human milk oligosaccharides	310:336	human milk oligosaccharides (hMOs)	310:343	Human milk also contains a plethora of bioactive molecules, including a large set of human milk oligosaccharides (hMOs).					
33954318	7	47	theme	prior	1288:1292	arg1	ETEC					1283:1286	ETEC	1283:1286	ETEC prior to addition to the Caco-2 cells	1283:1324	In addition, we reveal that DFβCD blocks adhesion of enterotoxigenic E. coli (ETEC) to Caco-2 cells, especially when DFβCD is pre-incubated with ETEC prior to addition to the Caco-2 cells.					
33954318	7	48	theme	Caco-2	1313:1318	arg1	cells					1320:1324	the Caco-2 cells	1309:1324	the Caco-2 cells	1309:1324	In addition, we reveal that DFβCD blocks adhesion of enterotoxigenic E. coli (ETEC) to Caco-2 cells, especially when DFβCD is pre-incubated with ETEC prior to addition to the Caco-2 cells.					
33954318	4	49	theme	mimics	696:701	arg1	"					702:702	so-called "mimics"	685:702	so-called "mimics"	685:702	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	4	49	theme	mimics	696:701	arg1	structures					673:682	hMO structures	669:682	hMO structures (so-called "mimics")	669:703	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	9	50	gly	fucosylated	1565:1575	arg1	glycans					1577:1583	fucosylated glycans	1565:1583	fucosylated glycans	1565:1583	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	1	51	theme	nutritional	203:213	arg1	features					215:222	nutritional features	203:222	nutritional features	203:222	Human milk is widely acknowledged as the best food for infants, and that is not just because of nutritional features.					
33954318	9	52	theme	other	1465:1469	arg1	hMOs					1483:1486	other fucosylated hMOs	1465:1486	other fucosylated hMOs	1465:1486	We expect that our results inspire the generation and biological evaluation of other fucosylated hMOs and mimics, to obtain a comprehensive overview of the anti-adhesive power of fucosylated glycans.					
33954318	5	53	theme	compound	893:900	arg1	DFβCD					929:933	DFβCD	929:933	DFβCD	929:933	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	5	53	theme	compound	893:900	arg1	di-fucosyl-β-cyclodextrin					902:926	the novel compound di-fucosyl-β-cyclodextrin	883:926	the novel compound di-fucosyl-β-cyclodextrin (DFβCD)	883:934	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	5	54	theme	novel	887:891	arg1	DFβCD					929:933	DFβCD	929:933	DFβCD	929:933	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	5	54	theme	novel	887:891	arg1	di-fucosyl-β-cyclodextrin					902:926	the novel compound di-fucosyl-β-cyclodextrin	883:926	the novel compound di-fucosyl-β-cyclodextrin (DFβCD)	883:934	Here we present our thorough study into the digestion, fermentation and anti-adhesive capacity of the novel compound di-fucosyl-β-cyclodextrin (DFβCD), which was inspired by the molecular structures of hMOs.					
33954318	4	55	theme	biological	765:774	arg1	effects					776:782	their biological effects	759:782	their biological effects	759:782	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	1	56	theme	best	148:151	arg1	food					153:156	the best food	144:156	the best food for infants	144:168	Human milk is widely acknowledged as the best food for infants, and that is not just because of nutritional features.					
33954318	1	56	theme	best	148:151	arg1	milk					113:116	Human milk	107:116	Human milk	107:116	Human milk is widely acknowledged as the best food for infants, and that is not just because of nutritional features.					
33954318	4	57	theme	medical	607:613	arg1	formula					623:629	(medical) infant formula	606:629	(medical) infant formula	606:629	Because hMOs are generally challenging to produce in sufficient quantities to study and ultimately apply in (medical) infant formula, novel compounds that are inspired by hMO structures (so-called "mimics") are interesting compounds to produce and evaluate for their biological effects.					
33954318	3	58	from	effects	422:428	arg1	pathogens					433:441	pathogens	433:441	pathogens	433:441	Especially fucosylated hMOs have received attention for their anti-adhesive effects on pathogens, preventing attachment to the intestine and infection.					
33954318	7	59	theme	coli	1210:1213	arg1	adhesion					1179:1186	adhesion	1179:1186	adhesion of enterotoxigenic E. coli (ETEC) to Caco-2 cells	1179:1236	In addition, we reveal that DFβCD blocks adhesion of enterotoxigenic E. coli (ETEC) to Caco-2 cells, especially when DFβCD is pre-incubated with ETEC prior to addition to the Caco-2 cells.					
33328348	10	0	theme	pattern	2311:2317	arg1	formation					2319:2327	rapid honeycomb pattern formation	2295:2327	rapid honeycomb pattern formation	2295:2327	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	9	1	from	biofilms	2237:2244	arg1	surfaces					2249:2256	surfaces	2249:2256	surfaces	2249:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	10	2	theme	first	2274:2278	arg1	description					2280:2290	this first description	2269:2290	this first description of rapid honeycomb pattern formation	2269:2327	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	10	3	theme	rapid	2295:2299	arg1	formation					2319:2327	rapid honeycomb pattern formation	2295:2327	rapid honeycomb pattern formation	2295:2327	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	9	4	from	formation	2224:2232	arg1	surfaces					2249:2256	surfaces	2249:2256	surfaces	2249:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	3	5	theme	biofilms	558:565	arg1	formation					545:553	the formation	541:553	the formation of biofilms on solid surfaces	541:583	In many bacteria, flagella and evolutionarily conserved type IV pili are required for the formation of biofilms on solid surfaces or floating at the air-liquid interface of liquid media.					
33328348	9	6	dep	provide	1986:1992	arg1	advances					1867:1874	advances	1867:1874	advances	1867:1874	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	9	7	theme	archaeon	1924:1931	arg1	Data					1952:1955	the model archaeon Haloferax volcanii Data	1914:1955	the model archaeon Haloferax volcanii Data gleaned from this study	1914:1979	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	2	8	theme	polymeric	272:280	arg1	substances					282:291	extracellular polymeric substances	258:291	extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while providing a structure that allows for optimal nutrient flow	258:452	Cells in a biofilm are encased in extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while providing a structure that allows for optimal nutrient flow.					
33328348	5	9	theme	model	832:836	arg1	volcanii					857:864	the model archaeon Haloferax volcanii	828:864	the model archaeon Haloferax volcanii	828:864	Additionally, in the model archaeon Haloferax volcanii, chemotaxis and AglB-dependent glycosylation play important roles in this process.					
33328348	9	10	theme	volcanii	1943:1950	arg1	Data					1952:1955	the model archaeon Haloferax volcanii Data	1914:1955	the model archaeon Haloferax volcanii Data gleaned from this study	1914:1979	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	5	11	theme	Haloferax	847:855	arg1	volcanii					857:864	the model archaeon Haloferax volcanii	828:864	the model archaeon Haloferax volcanii	828:864	Additionally, in the model archaeon Haloferax volcanii, chemotaxis and AglB-dependent glycosylation play important roles in this process.					
33328348	9	12	theme	novel	1662:1666	arg1	strategies					1682:1691	novel, unidentified strategies	1662:1691	novel, unidentified strategies for immersed liquid biofilm formation	1662:1729	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	3	13	theme	solid	570:574	arg1	surfaces					576:583	solid surfaces	570:583	solid surfaces	570:583	In many bacteria, flagella and evolutionarily conserved type IV pili are required for the formation of biofilms on solid surfaces or floating at the air-liquid interface of liquid media.					
33328348	10	14	theme	novel	2348:2352	arg1	insights					2354:2361	novel insights	2348:2361	novel insights into the underlying structural architecture of extracellular polymeric substances and cells	2348:2453	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	3	15	theme	air-liquid	604:613	arg1	interface					615:623	the air-liquid interface	600:623	the air-liquid interface of liquid media	600:639	In many bacteria, flagella and evolutionarily conserved type IV pili are required for the formation of biofilms on solid surfaces or floating at the air-liquid interface of liquid media.					
33328348	10	16	theme	underlying	2372:2381	arg1	architecture					2394:2405	the underlying structural architecture	2368:2405	the underlying structural architecture of extracellular polymeric substances and cells	2368:2453	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	9	17	theme	liquid	1706:1711	arg1	formation					1721:1729	immersed liquid biofilm formation	1697:1729	immersed liquid biofilm formation	1697:1729	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	8	18	theme	dish	1451:1454	arg1	lid					1456:1458	the petri dish lid	1441:1458	the petri dish lid	1441:1458	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	8	18	theme	dish	1451:1454	arg1	phenomenon					1463:1472	a phenomenon	1461:1472	a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity	1461:1593	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	8	18	theme	dish	1451:1454	arg1	dependent					1486:1494	dependent	1486:1494	dependent	1486:1494	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	9	19	from	surfaces	2249:2256	arg1	formation					2224:2232	the formation	2220:2232	the formation of biofilms on surfaces	2220:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	3	20	theme	media	635:639	arg1	interface					615:623	the air-liquid interface	600:623	the air-liquid interface of liquid media	600:639	In many bacteria, flagella and evolutionarily conserved type IV pili are required for the formation of biofilms on solid surfaces or floating at the air-liquid interface of liquid media.					
33328348	5	21	theme	important	916:924	arg1	roles					926:930	important roles	916:930	important roles	916:930	Additionally, in the model archaeon Haloferax volcanii, chemotaxis and AglB-dependent glycosylation play important roles in this process.					
33328348	10	22	theme	substances	2434:2443	arg1	architecture					2394:2405	the underlying structural architecture	2368:2405	the underlying structural architecture of extracellular polymeric substances and cells	2368:2453	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	7	23	theme	deletion	1209:1216	arg1	mutants					1218:1224	a chemotaxis-targeting transposon and aglB deletion mutants	1166:1224	mutants	1218:1224	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	7	24	theme	archaella	1144:1152	arg1	mutants					1218:1224	a chemotaxis-targeting transposon and aglB deletion mutants	1166:1224	mutants	1218:1224	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	7	24	theme	archaella	1144:1152	arg1	transposon					1189:1198	a chemotaxis-targeting transposon and aglB deletion mutants	1166:1224	transposon	1189:1198	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	7	24	theme	archaella	1144:1152	arg1	mutants					1059:1065	mutants	1059:1065	mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella	1059:1152	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	6	25	theme	liquid	993:998	arg1	cultures					1000:1007	liquid cultures	993:1007	liquid cultures poured into petri dishes	993:1032	H. volcanii also forms immersed biofilms in liquid cultures poured into petri dishes.					
33328348	10	26	theme	extracellular	2410:2422	arg1	substances					2434:2443	extracellular polymeric substances	2410:2443	extracellular polymeric substances	2410:2443	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	9	27	theme	future	2023:2028	arg1	studies					2030:2036	future studies	2023:2036	future studies	2023:2036	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	4	28	theme	type	699:702	arg1	pili					707:710	type IV pili	699:710	type IV pili	699:710	Similarly, in many archaea it has been demonstrated that type IV pili and, in a subset of these species, archaella are required for biofilm formation on solid surfaces.					
33328348	3	29	from	formation	545:553	arg1	surfaces					576:583	solid surfaces	570:583	solid surfaces	570:583	In many bacteria, flagella and evolutionarily conserved type IV pili are required for the formation of biofilms on solid surfaces or floating at the air-liquid interface of liquid media.					
33328348	9	30	theme	rapid	1749:1753	arg1	rearrangements.IMPORTANCE					1766:1790	rapid structural rearrangements.IMPORTANCE	1749:1790	rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1749:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	4	31	theme	species	738:744	arg1	subset					722:727	a subset	720:727	a subset of these species	720:744	Similarly, in many archaea it has been demonstrated that type IV pili and, in a subset of these species, archaella are required for biofilm formation on solid surfaces.					
33328348	4	31	theme	species	738:744	arg1	pili					707:710	type IV pili	699:710	type IV pili	699:710	Similarly, in many archaea it has been demonstrated that type IV pili and, in a subset of these species, archaella are required for biofilm formation on solid surfaces.					
33328348	7	32	theme	chemotaxis-targeting	1168:1187	arg1	transposon					1189:1198	a chemotaxis-targeting transposon and aglB deletion mutants	1166:1224	transposon	1189:1198	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	9	33	dep	biofilms	1858:1865	arg1	provide					1986:1992	provide	1986:1992	advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1867:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	9	34	from	biofilms	2089:2096	arg1	haloarchaeon					2106:2117	this haloarchaeon	2101:2117	this haloarchaeon	2101:2117	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	10	35	theme	cells	2449:2453	arg1	architecture					2394:2405	the underlying structural architecture	2368:2405	the underlying structural architecture of extracellular polymeric substances and cells	2368:2453	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	8	36	theme	liquid-based	1327:1338	arg1	biofilms					1340:1347	these liquid-based biofilms	1321:1347	these liquid-based biofilms	1321:1347	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	0	37	theme	Honeycomb	113:121	arg1	Formation					131:139	Rapid Honeycomb Pattern Formation	107:139	Rapid Honeycomb Pattern Formation	107:139	Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation.					
33328348	9	38	from	formation	2159:2167	arg1	general					2172:2178	general	2172:2178	general	2172:2178	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	9	39	theme	first	1797:1801	arg1	study					1824:1828	This first molecular biological study	1792:1828	rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1749:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	0	40	theme	Biofilm	75:81	arg1	Machineries					83:93	Known Biofilm Machineries	69:93	Known Biofilm Machineries	69:93	Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation.					
33328348	0	41	theme	Haloferax	0:8	arg1	volcanii					10:17	Haloferax volcanii	0:17	Haloferax volcanii Immersed Liquid Biofilms	0:42	Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation.					
33328348	9	42	theme	biological	1813:1822	arg1	study					1824:1828	This first molecular biological study	1792:1828	rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1749:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	9	43	theme	liquid	2082:2087	arg1	biofilms					2089:2096	immersed liquid biofilms	2073:2096	immersed liquid biofilms in this haloarchaeon	2073:2117	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	2	44	theme	nutrient	440:447	arg1	flow					449:452	optimal nutrient flow	432:452	optimal nutrient flow	432:452	Cells in a biofilm are encased in extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while providing a structure that allows for optimal nutrient flow.					
33328348	0	45	theme	Immersed	19:26	arg1	Biofilms					35:42	Haloferax volcanii Immersed Liquid Biofilms	0:42	Haloferax volcanii Immersed Liquid Biofilms	0:42	Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation.					
33328348	8	46	theme	oxygen	1519:1524	arg1	concentration					1526:1538	oxygen concentration	1519:1538	oxygen concentration	1519:1538	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	8	47	from	changes	1499:1505	arg1	light					1510:1514	light	1510:1514	light	1510:1514	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	8	47	from	changes	1499:1505	arg1	concentration					1526:1538	oxygen concentration	1519:1538	oxygen concentration	1519:1538	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	7	48	theme	pili	1136:1139	arg1	biosynthesis					1112:1123	the biosynthesis	1108:1123	the biosynthesis of type IV pili	1108:1139	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	6	49	theme	petri	1021:1025	arg1	dishes					1027:1032	petri dishes	1021:1032	petri dishes	1021:1032	H. volcanii also forms immersed biofilms in liquid cultures poured into petri dishes.					
33328348	9	50	theme	immersed	1842:1849	arg1	biofilms					1858:1865	archaeal immersed liquid biofilms	1833:1865	archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1833:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	0	51	theme	Known	69:73	arg1	Machineries					83:93	Known Biofilm Machineries	69:93	Known Biofilm Machineries	69:93	Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation.					
33328348	3	52	theme	type	511:514	arg1	pili					519:522	evolutionarily conserved type IV pili	486:522	evolutionarily conserved type IV pili	486:522	In many bacteria, flagella and evolutionarily conserved type IV pili are required for the formation of biofilms on solid surfaces or floating at the air-liquid interface of liquid media.					
33328348	7	53	theme	type	1128:1131	arg1	pili					1136:1139	type IV pili	1128:1139	type IV pili	1128:1139	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	9	54	theme	biofilms	1858:1865	arg1	study					1824:1828	This first molecular biological study	1792:1828	rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1749:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	2	55	theme	extracellular	258:270	arg1	substances					282:291	extracellular polymeric substances	258:291	extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while providing a structure that allows for optimal nutrient flow	258:452	Cells in a biofilm are encased in extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while providing a structure that allows for optimal nutrient flow.					
33328348	9	56	dep	components	2049:2058	arg1	required					2060:2067	required	2060:2067	required for immersed liquid biofilms in this haloarchaeon	2060:2117	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	7	57	theme	liquid	1270:1275	arg1	cultures					1277:1284	liquid cultures	1270:1284	liquid cultures	1270:1284	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	4	58	theme	biofilm	774:780	arg1	formation					782:790	biofilm formation	774:790	biofilm formation on solid surfaces	774:808	Similarly, in many archaea it has been demonstrated that type IV pili and, in a subset of these species, archaella are required for biofilm formation on solid surfaces.					
33328348	7	59	theme	obvious	1232:1238	arg1	defects					1240:1246	obvious defects	1232:1246	obvious defects in biofilms formed in liquid cultures	1232:1284	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	9	60	theme	biological	1886:1895	arg1	understanding					1897:1909	our basic biological understanding	1876:1909	our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study	1876:1979	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	9	61	theme	biofilm	2151:2157	arg1	formation					2159:2167	liquid biofilm formation	2144:2167	liquid biofilm formation	2144:2167	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	10	62	theme	honeycomb	2301:2309	arg1	formation					2319:2327	rapid honeycomb pattern formation	2295:2327	rapid honeycomb pattern formation	2295:2327	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	8	63	theme	humidity	1586:1593	arg1	reduction					1573:1581	controlled reduction	1562:1581	controlled reduction of humidity	1562:1593	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	9	64	theme	model	1918:1922	arg1	Data					1952:1955	the model archaeon Haloferax volcanii Data	1914:1955	the model archaeon Haloferax volcanii Data gleaned from this study	1914:1979	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	7	65	theme	haloarchaeon	1075:1086	arg1	mutants					1218:1224	a chemotaxis-targeting transposon and aglB deletion mutants	1166:1224	mutants	1218:1224	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	7	65	theme	haloarchaeon	1075:1086	arg1	transposon					1189:1198	a chemotaxis-targeting transposon and aglB deletion mutants	1166:1224	transposon	1189:1198	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	7	65	theme	haloarchaeon	1075:1086	arg1	mutants					1059:1065	mutants	1059:1065	mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella	1059:1152	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	5	66	theme	archaeon	838:845	arg1	volcanii					857:864	the model archaeon Haloferax volcanii	828:864	the model archaeon Haloferax volcanii	828:864	Additionally, in the model archaeon Haloferax volcanii, chemotaxis and AglB-dependent glycosylation play important roles in this process.					
33328348	7	67	from	defects	1240:1246	arg1	biofilms					1251:1258	biofilms	1251:1258	biofilms formed in liquid cultures	1251:1284	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	9	68	theme	Haloferax	1933:1941	arg1	Data					1952:1955	the model archaeon Haloferax volcanii Data	1914:1955	the model archaeon Haloferax volcanii Data gleaned from this study	1914:1979	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	4	69	theme	many	656:659	arg1	archaea					661:667	many archaea	656:667	many archaea	656:667	Similarly, in many archaea it has been demonstrated that type IV pili and, in a subset of these species, archaella are required for biofilm formation on solid surfaces.					
33328348	1	70	theme	many	184:187	arg1	microorganisms					189:202	many microorganisms	184:202	many microorganisms	184:202	The ability to form biofilms is shared by many microorganisms, including archaea.					
33328348	1	70	theme	many	184:187	arg1	archaea					215:221	archaea	215:221	archaea	215:221	The ability to form biofilms is shared by many microorganisms, including archaea.					
33328348	10	71	theme	formation	2319:2327	arg1	description					2280:2290	this first description	2269:2290	this first description of rapid honeycomb pattern formation	2269:2327	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	9	72	theme	Data	1952:1955	arg1	understanding					1897:1909	our basic biological understanding	1876:1909	our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study	1876:1979	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	4	73	theme	solid	795:799	arg1	surfaces					801:808	solid surfaces	795:808	solid surfaces	795:808	Similarly, in many archaea it has been demonstrated that type IV pili and, in a subset of these species, archaella are required for biofilm formation on solid surfaces.					
33328348	3	74	theme	IV	516:517	arg1	pili					519:522	evolutionarily conserved type IV pili	486:522	evolutionarily conserved type IV pili	486:522	In many bacteria, flagella and evolutionarily conserved type IV pili are required for the formation of biofilms on solid surfaces or floating at the air-liquid interface of liquid media.					
33328348	8	75	theme	petri	1445:1449	arg1	lid					1456:1458	the petri dish lid	1441:1458	the petri dish lid	1441:1458	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	8	75	theme	petri	1445:1449	arg1	phenomenon					1463:1472	a phenomenon	1461:1472	a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity	1461:1593	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	8	75	theme	petri	1445:1449	arg1	dependent					1486:1494	dependent	1486:1494	dependent	1486:1494	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	10	76	theme	immersed	2462:2469	arg1	biofilms					2478:2485	immersed liquid biofilms	2462:2485	immersed liquid biofilms	2462:2485	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	9	77	dep	rearrangements.IMPORTANCE	1766:1790	arg1	study					1824:1828	This first molecular biological study	1792:1828	rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1749:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	5	78	theme	AglB-dependent	882:895	arg1	glycosylation					897:909	AglB-dependent glycosylation	882:909	AglB-dependent glycosylation	882:909	Additionally, in the model archaeon Haloferax volcanii, chemotaxis and AglB-dependent glycosylation play important roles in this process.					
33328348	9	79	theme	immersed	1697:1704	arg1	formation					1721:1729	immersed liquid biofilm formation	1697:1729	immersed liquid biofilm formation	1697:1729	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	8	80	theme	lid	1456:1458	arg1	removal					1430:1436	removal	1430:1436	removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity	1430:1593	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	4	81	theme	IV	704:705	arg1	pili					707:710	type IV pili	699:710	type IV pili	699:710	Similarly, in many archaea it has been demonstrated that type IV pili and, in a subset of these species, archaella are required for biofilm formation on solid surfaces.					
33328348	9	82	theme	biofilms	2237:2244	arg1	formation					2224:2232	the formation	2220:2232	the formation of biofilms on surfaces	2220:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	3	83	theme	liquid	628:633	arg1	media					635:639	liquid media	628:639	liquid media	628:639	In many bacteria, flagella and evolutionarily conserved type IV pili are required for the formation of biofilms on solid surfaces or floating at the air-liquid interface of liquid media.					
33328348	4	84	from	formation	782:790	arg1	surfaces					801:808	solid surfaces	795:808	solid surfaces	795:808	Similarly, in many archaea it has been demonstrated that type IV pili and, in a subset of these species, archaella are required for biofilm formation on solid surfaces.					
33328348	9	85	theme	biofilm	1713:1719	arg1	formation					1721:1729	immersed liquid biofilm formation	1697:1729	immersed liquid biofilm formation	1697:1729	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	9	86	theme	invaluable	1997:2006	arg1	foundation					2008:2017	an invaluable foundation	1994:2017	an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1994:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	7	87	theme	aglB	1204:1207	arg1	mutants					1218:1224	a chemotaxis-targeting transposon and aglB deletion mutants	1166:1224	mutants	1218:1224	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	10	88	theme	polymeric	2424:2432	arg1	substances					2434:2443	extracellular polymeric substances	2410:2443	extracellular polymeric substances	2410:2443	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	9	89	dep	novel	1662:1666	arg1	unidentified					1669:1680	unidentified	1669:1680	unidentified	1669:1680	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	10	90	theme	structural	2383:2392	arg1	architecture					2394:2405	the underlying structural architecture	2368:2405	the underlying structural architecture of extracellular polymeric substances and cells	2368:2453	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	0	91	theme	Rapid	107:111	arg1	Formation					131:139	Rapid Honeycomb Pattern Formation	107:139	Rapid Honeycomb Pattern Formation	107:139	Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation.					
33328348	6	92	theme	immersed	972:979	arg1	biofilms					981:988	immersed biofilms	972:988	immersed biofilms	972:988	H. volcanii also forms immersed biofilms in liquid cultures poured into petri dishes.					
33328348	9	93	theme	structural	1755:1764	arg1	rearrangements.IMPORTANCE					1766:1790	rapid structural rearrangements.IMPORTANCE	1749:1790	rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1749:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	0	94	theme	Pattern	123:129	arg1	Formation					131:139	Rapid Honeycomb Pattern Formation	107:139	Rapid Honeycomb Pattern Formation	107:139	Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation.					
33328348	2	95	from	Cells	224:228	arg1	biofilm					235:241	a biofilm	233:241	a biofilm	233:241	Cells in a biofilm are encased in extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while providing a structure that allows for optimal nutrient flow.					
33328348	3	96	theme	many	458:461	arg1	bacteria					463:470	many bacteria	458:470	many bacteria	458:470	In many bacteria, flagella and evolutionarily conserved type IV pili are required for the formation of biofilms on solid surfaces or floating at the air-liquid interface of liquid media.					
33328348	0	97	theme	volcanii	10:17	arg1	Biofilms					35:42	Haloferax volcanii Immersed Liquid Biofilms	0:42	Haloferax volcanii Immersed Liquid Biofilms	0:42	Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation.					
33328348	9	98	theme	molecular	1803:1811	arg1	study					1824:1828	This first molecular biological study	1792:1828	rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1749:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	9	99	theme	immersed	2073:2080	arg1	biofilms					2089:2096	immersed liquid biofilms	2073:2096	immersed liquid biofilms in this haloarchaeon	2073:2117	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	0	100	theme	Liquid	28:33	arg1	Biofilms					35:42	Haloferax volcanii Immersed Liquid Biofilms	0:42	Haloferax volcanii Immersed Liquid Biofilms	0:42	Haloferax volcanii Immersed Liquid Biofilms Develop Independently of Known Biofilm Machineries and Exhibit Rapid Honeycomb Pattern Formation.					
33328348	10	101	theme	liquid	2471:2476	arg1	biofilms					2478:2485	immersed liquid biofilms	2462:2485	immersed liquid biofilms	2462:2485	Moreover, this first description of rapid honeycomb pattern formation is likely to yield novel insights into the underlying structural architecture of extracellular polymeric substances and cells within immersed liquid biofilms.					
33328348	2	102	theme	optimal	432:438	arg1	flow					449:452	optimal nutrient flow	432:452	optimal nutrient flow	432:452	Cells in a biofilm are encased in extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while providing a structure that allows for optimal nutrient flow.					
33328348	7	103	theme	IV	1133:1134	arg1	pili					1136:1139	type IV pili	1128:1139	type IV pili	1128:1139	This study reveals that mutants of this haloarchaeon that interfere with the biosynthesis of type IV pili or archaella, as well as a chemotaxis-targeting transposon and aglB deletion mutants, lack obvious defects in biofilms formed in liquid cultures.					
33328348	9	104	theme	archaeal	1833:1840	arg1	biofilms					1858:1865	archaeal immersed liquid biofilms	1833:1865	archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1833:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	8	105	theme	rearrangement	1364:1376	arg1	capable					1353:1359	capable	1353:1359	capable	1353:1359	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	9	106	theme	liquid	1851:1856	arg1	biofilms					1858:1865	archaeal immersed liquid biofilms	1833:1865	archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces	1833:2256	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	8	107	theme	honeycomb-like	1383:1396	arg1	patterns					1398:1405	honeycomb-like patterns	1383:1405	honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity	1383:1593	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	2	108	theme	extracellular	347:359	arg1	DNA					361:363	extracellular DNA	347:363	extracellular DNA	347:363	Cells in a biofilm are encased in extracellular polymeric substances that typically include polysaccharides, proteins, and extracellular DNA, conferring protection while providing a structure that allows for optimal nutrient flow.					
33328348	8	109	theme	controlled	1562:1571	arg1	reduction					1573:1581	controlled reduction	1562:1581	controlled reduction of humidity	1562:1593	Strikingly, we have observed that these liquid-based biofilms are capable of rearrangement into honeycomb-like patterns that rapidly form upon removal of the petri dish lid, a phenomenon that is not dependent on changes in light or oxygen concentration but can be induced by controlled reduction of humidity.					
33328348	3	110	theme	conserved	501:509	arg1	pili					519:522	evolutionarily conserved type IV pili	486:522	evolutionarily conserved type IV pili	486:522	In many bacteria, flagella and evolutionarily conserved type IV pili are required for the formation of biofilms on solid surfaces or floating at the air-liquid interface of liquid media.					
33328348	9	111	theme	basic	1880:1884	arg1	understanding					1897:1909	our basic biological understanding	1876:1909	our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study	1876:1979	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
33328348	9	112	theme	liquid	2144:2149	arg1	formation					2159:2167	liquid biofilm formation	2144:2167	liquid biofilm formation	2144:2167	Taken together, this study demonstrates that H. volcanii requires novel, unidentified strategies for immersed liquid biofilm formation and also exhibits rapid structural rearrangements.IMPORTANCE This first molecular biological study of archaeal immersed liquid biofilms advances our basic biological understanding of the model archaeon Haloferax volcanii Data gleaned from this study also provide an invaluable foundation for future studies to uncover components required for immersed liquid biofilms in this haloarchaeon and also potentially for liquid biofilm formation in general, which is poorly understood compared to the formation of biofilms on surfaces.					
34083726	6	0	theme	terminal	1234:1241	arg1	modifications					1243:1255	terminal modifications	1234:1255	terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation	1234:1341	The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.					
34083726	1	1	gly	sialylated	185:194	arg1	O-glycans					141:149	O-glycans	141:149	O-glycans	141:149	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	1	gly	sialylated	185:194	arg1	N-					134:135	N-	134:135	N-	134:135	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	1	gly	sialylated	185:194	arg1	residues					122:129	The terminal galactose residues	99:129	The terminal galactose residues of N- and O-glycans in animal glycoproteins	99:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	3	2	theme	ligand-bound	697:708	arg1	structure					718:726	ligand-bound crystal structure	697:726	ligand-bound crystal structure	697:726	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	3	theme	crystal	710:716	arg1	structure					718:726	ligand-bound crystal structure	697:726	ligand-bound crystal structure	697:726	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	4	4	theme	glycoproteins	850:862	arg1	N-glycans					814:822	N-glycans	814:822	N-glycans of many plasma and tissue glycoproteins	814:862	3-O-SGal is expressed on N-glycans of many plasma and tissue glycoproteins, but recognition by O6 is often masked by sialic acid and thus exposed by treatment with neuraminidase.					
34083726	1	5	from	glycoproteins	161:173	arg1	O-glycans					141:149	O-glycans	141:149	O-glycans	141:149	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	5	from	glycoproteins	161:173	arg1	N-					134:135	N-	134:135	N-	134:135	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	5	from	glycoproteins	161:173	arg1	residues					122:129	The terminal galactose residues	99:129	The terminal galactose residues of N- and O-glycans in animal glycoproteins	99:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	5	6	theme	sulfotransferases	1044:1060	arg1	expression					1018:1027	expression	1018:1027	expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3)	1018:1084	O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3).					
34083726	5	7	with	consistent	1002:1011	arg1	expression					1018:1027	expression	1018:1027	expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3)	1018:1084	O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3).					
34083726	6	8	theme	glycans	1260:1266	arg1	modifications					1243:1255	terminal modifications	1234:1255	terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation	1234:1341	The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.					
34083726	5	9	dep	sulfotransferases	1044:1060	arg1	GAL3ST-3					1076:1083	GAL3ST-3	1076:1083	GAL3ST-3	1076:1083	O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3).					
34083726	5	9	dep	sulfotransferases	1044:1060	arg1	GAL3ST-2					1063:1070	GAL3ST-2	1063:1070	GAL3ST-2	1063:1070	O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3).					
34083726	5	9	dep	sulfotransferases	1044:1060	arg1	sulfotransferases					1044:1060	the cognate sulfotransferases	1032:1060	the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3)	1032:1084	O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3).					
34083726	0	10	from	epitopes	62:69	arg1	glycoproteins					84:96	mammalian glycoproteins	74:96	mammalian glycoproteins	74:96	Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins.					
34083726	6	11	theme	new	1158:1160	arg1	biomarkers					1162:1171	new biomarkers	1158:1171	new biomarkers for disease	1158:1183	The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.					
34083726	4	12	theme	plasma	832:837	arg1	glycoproteins					850:862	many plasma and tissue glycoproteins	827:862	many plasma and tissue glycoproteins	827:862	3-O-SGal is expressed on N-glycans of many plasma and tissue glycoproteins, but recognition by O6 is often masked by sialic acid and thus exposed by treatment with neuraminidase.					
34083726	0	13	gly	glycoproteins	84:96	arg1	glycoproteins					84:96	mammalian glycoproteins	74:96	mammalian glycoproteins	74:96	Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins.					
34083726	4	14	gly	glycoproteins	850:862	arg1	glycoproteins					850:862	many plasma and tissue glycoproteins	827:862	many plasma and tissue glycoproteins	827:862	3-O-SGal is expressed on N-glycans of many plasma and tissue glycoproteins, but recognition by O6 is often masked by sialic acid and thus exposed by treatment with neuraminidase.					
34083726	4	15	theme	many	827:830	arg1	glycoproteins					850:862	many plasma and tissue glycoproteins	827:862	many plasma and tissue glycoproteins	827:862	3-O-SGal is expressed on N-glycans of many plasma and tissue glycoproteins, but recognition by O6 is often masked by sialic acid and thus exposed by treatment with neuraminidase.					
34083726	6	16	theme	3-O-SGal	1124:1131	arg1	expression					1133:1142	3-O-SGal expression	1124:1142	3-O-SGal expression	1124:1142	The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.					
34083726	2	17	theme	sea	375:377	arg1	lamprey					379:385	a novel sea lamprey variable lymphocyte receptor (VLR)	367:420	a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression	367:461	To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression.					
34083726	0	18	theme	lamprey	6:12	arg1	antibody					14:21	Novel lamprey antibody	0:21	Novel lamprey antibody	0:21	Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins.					
34083726	3	19	theme	murine	499:504	arg1	chimera					510:516	a recombinant murine IgG chimera	485:516	a recombinant murine IgG chimera	485:516	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	19	theme	murine	499:504	arg1	O6					464:465	O6	464:465	O6	464:465	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	4	20	theme	tissue	843:848	arg1	glycoproteins					850:862	many plasma and tissue glycoproteins	827:862	many plasma and tissue glycoproteins	827:862	3-O-SGal is expressed on N-glycans of many plasma and tissue glycoproteins, but recognition by O6 is often masked by sialic acid and thus exposed by treatment with neuraminidase.					
34083726	1	21	from	O-glycans	141:149	arg1	glycoproteins					161:173	animal glycoproteins	154:173	animal glycoproteins	154:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	2	22	theme	novel	369:373	arg1	lamprey					379:385	a novel sea lamprey variable lymphocyte receptor (VLR)	367:420	a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression	367:461	To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression.					
34083726	0	23	theme	Novel	0:4	arg1	antibody					14:21	Novel lamprey antibody	0:21	Novel lamprey antibody	0:21	Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins.					
34083726	6	24	theme	modifications	1243:1255	arg1	roles					1225:1229	the functional roles	1210:1229	the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation	1210:1341	The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.					
34083726	1	25	theme	terminal	103:110	arg1	O-glycans					141:149	O-glycans	141:149	O-glycans	141:149	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	25	theme	terminal	103:110	arg1	N-					134:135	N-	134:135	N-	134:135	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	25	theme	terminal	103:110	arg1	residues					122:129	The terminal galactose residues	99:129	The terminal galactose residues of N- and O-glycans in animal glycoproteins	99:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	5	26	theme	many	982:985	arg1	tissues					993:999	many human tissues	982:999	many human tissues	982:999	O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3).					
34083726	1	27	from	N-	134:135	arg1	glycoproteins					161:173	animal glycoproteins	154:173	animal glycoproteins	154:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	28	theme	galactose	112:120	arg1	O-glycans					141:149	O-glycans	141:149	O-glycans	141:149	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	28	theme	galactose	112:120	arg1	N-					134:135	N-	134:135	N-	134:135	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	28	theme	galactose	112:120	arg1	residues					122:129	The terminal galactose residues	99:129	The terminal galactose residues of N- and O-glycans in animal glycoproteins	99:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	6	29	theme	functional	1214:1223	arg1	roles					1225:1229	the functional roles	1210:1229	the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation	1210:1341	The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.					
34083726	1	30	theme	additional	288:297	arg1	modification					322:333	an additional, but poorly understood modification	285:333	an additional, but poorly understood modification	285:333	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	3	31	theme	IgG	506:508	arg1	chimera					510:516	a recombinant murine IgG chimera	485:516	a recombinant murine IgG chimera	485:516	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	31	theme	IgG	506:508	arg1	O6					464:465	O6	464:465	O6	464:465	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	4	32	theme	sialic	906:911	arg1	acid					913:916	sialic acid	906:916	sialic acid	906:916	3-O-SGal is expressed on N-glycans of many plasma and tissue glycoproteins, but recognition by O6 is often masked by sialic acid and thus exposed by treatment with neuraminidase.					
34083726	0	33	theme	sulfated	43:50	arg1	epitopes					62:69	terminal sulfated galactose epitopes	34:69	terminal sulfated galactose epitopes on mammalian glycoproteins	34:96	Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins.					
34083726	6	34	theme	O6	1107:1108	arg1	availability					1091:1102	The availability	1087:1102	The availability of O6 for exploring 3-O-SGal expression	1087:1142	The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.					
34083726	6	35	theme	relationships	1272:1284	arg1	modifications					1243:1255	terminal modifications	1234:1255	terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation	1234:1341	The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.					
34083726	2	36	theme	lymphocyte	396:405	arg1	VLR					417:419	VLR	417:419	VLR	417:419	To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression.					
34083726	2	36	theme	lymphocyte	396:405	arg1	receptor					407:414	variable lymphocyte receptor	387:414	a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression	367:461	To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression.					
34083726	0	37	theme	terminal	34:41	arg1	epitopes					62:69	terminal sulfated galactose epitopes	34:69	terminal sulfated galactose epitopes on mammalian glycoproteins	34:96	Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins.					
34083726	3	38	theme	human	763:767	arg1	macroarrays					776:786	human tissue macroarrays	763:786	human tissue macroarrays	763:786	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	2	39	theme	3-O-SGal	443:450	arg1	expression					452:461	3-O-SGal expression	443:461	3-O-SGal expression	443:461	To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression.					
34083726	1	40	dep	such	231:234	arg1	as					236:237	as	236:237	as	236:237	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	3	41	theme	macroarrays	776:786	arg1	immunohistochemistry					739:758	immunohistochemistry	739:758	immunohistochemistry of human tissue macroarrays	739:786	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	41	theme	macroarrays	776:786	arg1	structure					718:726	ligand-bound crystal structure	697:726	ligand-bound crystal structure	697:726	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	41	theme	macroarrays	776:786	arg1	analyses					663:670	glycoprotein microarray analyses	639:670	glycoprotein microarray analyses	639:670	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	41	theme	macroarrays	776:786	arg1	calorimetry					684:694	isothermal calorimetry	673:694	isothermal calorimetry	673:694	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	41	theme	macroarrays	776:786	arg1	FACS					729:732	FACS	729:732	FACS	729:732	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	41	theme	macroarrays	776:786	arg1	glycan					628:633	glycan	628:633	glycan	628:633	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	2	42	theme	variable	387:394	arg1	VLR					417:419	VLR	417:419	VLR	417:419	To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression.					
34083726	2	42	theme	variable	387:394	arg1	receptor					407:414	variable lymphocyte receptor	387:414	a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression	367:461	To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression.					
34083726	3	43	theme	tissue	769:774	arg1	macroarrays					776:786	human tissue macroarrays	763:786	human tissue macroarrays	763:786	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	44	theme	approaches	606:615	arg1	approaches					606:615	approaches	606:615	approaches	606:615	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	44	theme	approaches	606:615	arg1	immunohistochemistry					739:758	immunohistochemistry	739:758	immunohistochemistry of human tissue macroarrays	739:786	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	44	theme	approaches	606:615	arg1	structure					718:726	ligand-bound crystal structure	697:726	ligand-bound crystal structure	697:726	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	44	theme	approaches	606:615	arg1	analyses					663:670	glycoprotein microarray analyses	639:670	glycoprotein microarray analyses	639:670	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	44	theme	approaches	606:615	arg1	calorimetry					684:694	isothermal calorimetry	673:694	isothermal calorimetry	673:694	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	44	theme	approaches	606:615	arg1	FACS					729:732	FACS	729:732	FACS	729:732	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	44	theme	approaches	606:615	arg1	glycan					628:633	glycan	628:633	glycan	628:633	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	44	theme	approaches	606:615	arg1	variety					595:601	a variety	593:601	a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays	593:786	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	0	45	theme	galactose	52:60	arg1	epitopes					62:69	terminal sulfated galactose epitopes	34:69	terminal sulfated galactose epitopes on mammalian glycoproteins	34:96	Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins.					
34083726	1	46	theme	understood	311:320	arg1	modification					322:333	an additional, but poorly understood modification	285:333	an additional, but poorly understood modification	285:333	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	3	47	theme	glycoprotein	639:650	arg1	analyses					663:670	glycoprotein microarray analyses	639:670	glycoprotein microarray analyses	639:670	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	48	theme	3-O-SGal	558:565	arg1	epitope					567:573	the 3-O-SGal epitope	554:573	the 3-O-SGal epitope	554:573	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	1	49	theme	N-	134:135	arg1	O-glycans					141:149	O-glycans	141:149	O-glycans	141:149	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	49	theme	N-	134:135	arg1	N-					134:135	N-	134:135	N-	134:135	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	49	theme	N-	134:135	arg1	residues					122:129	The terminal galactose residues	99:129	The terminal galactose residues of N- and O-glycans in animal glycoproteins	99:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	6	50	theme	terminal	1294:1301	arg1	sulfation					1303:1311	terminal sulfation	1294:1311	terminal sulfation	1294:1311	The availability of O6 for exploring 3-O-SGal expression could lead to new biomarkers for disease and aid in understanding the functional roles of terminal modifications of glycans and relationships between terminal sulfation, sialylation and fucosylation.					
34083726	3	51	theme	microarray	652:661	arg1	analyses					663:670	glycoprotein microarray analyses	639:670	glycoprotein microarray analyses	639:670	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	4	52	with	treatment	938:946	arg1	neuraminidase					953:965	neuraminidase	953:965	neuraminidase	953:965	3-O-SGal is expressed on N-glycans of many plasma and tissue glycoproteins, but recognition by O6 is often masked by sialic acid and thus exposed by treatment with neuraminidase.					
34083726	1	53	from	residues	122:129	arg1	glycoproteins					161:173	animal glycoproteins	154:173	animal glycoproteins	154:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	54	gly	glycoproteins	161:173	arg1	glycoproteins					161:173	animal glycoproteins	154:173	animal glycoproteins	154:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	55	theme	O-glycans	141:149	arg1	O-glycans					141:149	O-glycans	141:149	O-glycans	141:149	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	55	theme	O-glycans	141:149	arg1	N-					134:135	N-	134:135	N-	134:135	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	55	theme	O-glycans	141:149	arg1	residues					122:129	The terminal galactose residues	99:129	The terminal galactose residues of N- and O-glycans in animal glycoproteins	99:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	3	56	gly	glycoprotein	639:650	arg1	glycoprotein					639:650	glycoprotein microarray analyses	639:670	glycoprotein microarray analyses	639:670	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	0	57	theme	mammalian	74:82	arg1	glycoproteins					84:96	mammalian glycoproteins	74:96	mammalian glycoproteins	74:96	Novel lamprey antibody recognizes terminal sulfated galactose epitopes on mammalian glycoproteins.					
34083726	5	58	theme	cognate	1036:1042	arg1	GAL3ST-3					1076:1083	GAL3ST-3	1076:1083	GAL3ST-3	1076:1083	O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3).					
34083726	5	58	theme	cognate	1036:1042	arg1	GAL3ST-2					1063:1070	GAL3ST-2	1063:1070	GAL3ST-2	1063:1070	O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3).					
34083726	5	58	theme	cognate	1036:1042	arg1	sulfotransferases					1044:1060	the cognate sulfotransferases	1032:1060	the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3)	1032:1084	O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3).					
34083726	2	59	dep	lamprey	379:385	arg1	VLR					417:419	VLR	417:419	VLR	417:419	To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression.					
34083726	2	59	dep	lamprey	379:385	arg1	receptor					407:414	variable lymphocyte receptor	387:414	a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression	367:461	To this end, we have developed a novel sea lamprey variable lymphocyte receptor (VLR) termed O6 to explore 3-O-SGal expression.					
34083726	3	60	theme	recombinant	487:497	arg1	chimera					510:516	a recombinant murine IgG chimera	485:516	a recombinant murine IgG chimera	485:516	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	3	60	theme	recombinant	487:497	arg1	O6					464:465	O6	464:465	O6	464:465	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	1	61	theme	3-O-sulfated	239:250	arg1	3-O-SGal					263:270	3-O-SGal	263:270	3-O-SGal	263:270	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	1	61	theme	3-O-sulfated	239:250	arg1	galactose					252:260	3-O-sulfated galactose	239:260	3-O-sulfated galactose (3-O-SGal)	239:271	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
34083726	3	62	theme	isothermal	673:682	arg1	calorimetry					684:694	isothermal calorimetry	673:694	isothermal calorimetry	673:694	O6 was engineered as a recombinant murine IgG chimera and its specificity and affinity to the 3-O-SGal epitope was defined using a variety of approaches, including glycan and glycoprotein microarray analyses, isothermal calorimetry, ligand-bound crystal structure, FACS, and immunohistochemistry of human tissue macroarrays.					
34083726	5	63	theme	human	987:991	arg1	tissues					993:999	many human tissues	982:999	many human tissues	982:999	O6 recognizes many human tissues, consistent with expression of the cognate sulfotransferases (GAL3ST-2 and GAL3ST-3).					
34083726	1	64	theme	animal	154:159	arg1	glycoproteins					161:173	animal glycoproteins	154:173	animal glycoproteins	154:173	The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an additional, but poorly understood modification.					
32223173	1	0	theme	peptide	175:181	arg1	sequence					192:199	the peptide backbone sequence	171:199	the peptide backbone sequence	171:199	Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties.					
32223173	5	1	gly	glycopeptide	827:838	arg2	glycopeptide					827:838	a glycopeptide	825:838	a glycopeptide	825:838	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	1	2	theme	backbone	183:190	arg1	sequence					192:199	the peptide backbone sequence	171:199	the peptide backbone sequence	171:199	Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties.					
32223173	5	3	from	influence	764:772	arg1	time					817:820	the retention time	803:820	the retention time of a glycopeptide	803:838	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	2	4	theme	same	365:368	arg1	spectrum					370:377	the same spectrum	361:377	the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination	361:480	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	5	5	theme	incorrect	934:942	arg1	cases					944:948	over 400 incorrect cases	925:948	over 400 incorrect cases	925:948	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	6	6	theme	line	1094:1097	arg1	program					1110:1116	a command line executable program	1084:1116	a command line executable program	1084:1116	We make this technique available as a command line executable program, written in Python and C, freely available at https://github.com/mobiusklein/glycresoft in source form, with precompiled binaries for Windows.					
32223173	1	7	theme	moieties	268:275	arg1	fragmentation					246:258	the incomplete fragmentation	231:258	the incomplete fragmentation of both moieties	231:275	Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties.					
32223173	5	8	dep	validate	864:871	arg1	correcting					978:987	correcting	978:987	correcting 75 cases that could not be identified based on mass alone	978:1045	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	5	8	dep	validate	864:871	arg1	detecting					915:923	detecting	915:923	detecting over 400 incorrect cases during the MS/MS search	915:972	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	3	9	theme	few	552:554	arg1	inroads					556:562	few inroads	552:562	few inroads	552:562	To date, approaches to solving these problems have been limited, and few inroads have been made to address these issues.					
32223173	2	10	from	determination	468:480	arg1	spectrum					431:438	the mass spectrum	422:438	the mass spectrum	422:438	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	5	11	theme	glycan	781:786	arg1	composition					788:798	the glycan composition	777:798	the glycan composition	777:798	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	6	12	from	//github.com/mobiusklein/glycresoft	1170:1204	arg1	form					1216:1219	source form	1209:1219	source form	1209:1219	We make this technique available as a command line executable program, written in Python and C, freely available at https://github.com/mobiusklein/glycresoft in source form, with precompiled binaries for Windows.					
32223173	1	13	theme	sequence	192:199	arg1	identification					153:166	the identification	149:166	the identification of the peptide backbone sequence and the attached glycan(s)	149:226	Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties.					
32223173	5	14	theme	linear	726:731	arg1	approach					742:749	a linear modeling approach	724:749	a linear modeling approach	724:749	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	0	15	theme	Relative	0:7	arg1	Estimation					24:33	Relative Retention Time Estimation	0:33	Relative Retention Time Estimation	0:33	Relative Retention Time Estimation Improves N-Glycopeptide Identifications by LC-MS/MS.					
32223173	2	16	theme	missing	399:405	arg1	information					407:417	missing information	399:417	missing information in the mass spectrum	399:438	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	0	17	theme	Retention	9:17	arg1	Estimation					24:33	Relative Retention Time Estimation	0:33	Relative Retention Time Estimation	0:33	Relative Retention Time Estimation Improves N-Glycopeptide Identifications by LC-MS/MS.					
32223173	6	18	theme	source	1209:1214	arg1	form					1216:1219	source form	1209:1219	source form	1209:1219	We make this technique available as a command line executable program, written in Python and C, freely available at https://github.com/mobiusklein/glycresoft in source form, with precompiled binaries for Windows.					
32223173	2	19	theme	multiple	327:334	arg1	structures					336:345	multiple structures	327:345	multiple structures	327:345	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	2	20	theme	precursor	453:461	arg1	determination					468:480	incorrect precursor mass determination	443:480	incorrect precursor mass determination	443:480	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	1	21	theme	tandem	116:121	arg1	spectrometry					128:139	tandem mass spectrometry	116:139	tandem mass spectrometry	116:139	Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties.					
32223173	2	22	theme	due	392:394	arg1	spectrum					370:377	the same spectrum	361:377	the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination	361:480	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	2	23	theme	incorrect	443:451	arg1	determination					468:480	incorrect precursor mass determination	443:480	incorrect precursor mass determination	443:480	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	5	24	theme	composition	788:798	arg1	influence					764:772	the influence	760:772	the influence of the glycan composition on the retention time of a glycopeptide	760:838	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	1	25	theme	mass	123:126	arg1	spectrometry					128:139	tandem mass spectrometry	116:139	tandem mass spectrometry	116:139	Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties.					
32223173	1	26	theme	glycan	218:223	arg1	s					225:225	the attached glycan(s)	205:226	the attached glycan(s)	205:226	Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties.					
32223173	0	27	theme	Time	19:22	arg1	Estimation					24:33	Relative Retention Time Estimation	0:33	Relative Retention Time Estimation	0:33	Relative Retention Time Estimation Improves N-Glycopeptide Identifications by LC-MS/MS.					
32223173	2	28	theme	ambiguous	295:303	arg1	identifications					305:319	ambiguous identifications	295:319	ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination	295:480	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	2	29	theme	mass	426:429	arg1	spectrum					431:438	the mass spectrum	422:438	the mass spectrum	422:438	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	0	30	theme	N-Glycopeptide	44:57	arg1	Identifications					59:73	N-Glycopeptide Identifications	44:73	N-Glycopeptide Identifications	44:73	Relative Retention Time Estimation Improves N-Glycopeptide Identifications by LC-MS/MS.					
32223173	1	31	theme	attached	209:216	arg1	s					225:225	the attached glycan(s)	205:226	the attached glycan(s)	205:226	Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties.					
32223173	1	32	theme	s	225:225	arg1	identification					153:166	the identification	149:166	the identification of the peptide backbone sequence and the attached glycan(s)	149:226	Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties.					
32223173	5	33	theme	glycopeptide	827:838	arg1	time					817:820	the retention time	803:820	the retention time of a glycopeptide	803:838	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	4	34	attach	present	607:613	arg2	We					604:605	We	604:605	We	604:605	We present a technique to address some of these challenges and demonstrate it on previously published data sets.					
32223173	4	34	attach	present	607:613	arg1	sets					711:714	previously published data sets	685:714	previously published data sets	685:714	We present a technique to address some of these challenges and demonstrate it on previously published data sets.					
32223173	4	35	theme	data	706:709	arg1	sets					711:714	previously published data sets	685:714	previously published data sets	685:714	We present a technique to address some of these challenges and demonstrate it on previously published data sets.					
32223173	2	36	from	information	407:417	arg1	spectrum					431:438	the mass spectrum	422:438	the mass spectrum	422:438	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	4	37	theme	published	696:704	arg1	sets					711:714	previously published data sets	685:714	previously published data sets	685:714	We present a technique to address some of these challenges and demonstrate it on previously published data sets.					
32223173	6	38	from	https	1164:1168	arg1	available					1151:1159	available	1151:1159	available	1151:1159	We make this technique available as a command line executable program, written in Python and C, freely available at https://github.com/mobiusklein/glycresoft in source form, with precompiled binaries for Windows.					
32223173	6	39	with	program	1110:1116	arg1	binaries					1239:1246	precompiled binaries	1227:1246	precompiled binaries for Windows	1227:1258	We make this technique available as a command line executable program, written in Python and C, freely available at https://github.com/mobiusklein/glycresoft in source form, with precompiled binaries for Windows.					
32223173	5	40	gly	glycopeptides	873:885	arg2	glycopeptides					873:885	glycopeptides	873:885	glycopeptides	873:885	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	6	41	theme	available	1151:1159	arg1	program					1110:1116	a command line executable program	1084:1116	a command line executable program	1084:1116	We make this technique available as a command line executable program, written in Python and C, freely available at https://github.com/mobiusklein/glycresoft in source form, with precompiled binaries for Windows.					
32223173	5	42	theme	MS/MS	961:965	arg1	search					967:972	the MS/MS search	957:972	the MS/MS search	957:972	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	5	43	theme	same	898:901	arg1	experiment					903:912	the same experiment	894:912	the same experiment	894:912	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	6	44	theme	executable	1099:1108	arg1	program					1110:1116	a command line executable program	1084:1116	a command line executable program	1084:1116	We make this technique available as a command line executable program, written in Python and C, freely available at https://github.com/mobiusklein/glycresoft in source form, with precompiled binaries for Windows.					
32223173	6	45	theme	command	1086:1092	arg1	program					1110:1116	a command line executable program	1084:1116	a command line executable program	1084:1116	We make this technique available as a command line executable program, written in Python and C, freely available at https://github.com/mobiusklein/glycresoft in source form, with precompiled binaries for Windows.					
32223173	5	46	theme	modeling	733:740	arg1	approach					742:749	a linear modeling approach	724:749	a linear modeling approach	724:749	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	5	47	theme	retention	807:815	arg1	time					817:820	the retention time	803:820	the retention time of a glycopeptide	803:838	We use a linear modeling approach to learn the influence of the glycan composition on the retention time of a glycopeptide and use these models to validate glycopeptides within the same experiment, detecting over 400 incorrect cases during the MS/MS search and correcting 75 cases that could not be identified based on mass alone.					
32223173	6	48	dep	https	1164:1168	arg1	//github.com/mobiusklein/glycresoft					1170:1204	//github.com/mobiusklein/glycresoft	1170:1204	https://github.com/mobiusklein/glycresoft in source form	1164:1219	We make this technique available as a command line executable program, written in Python and C, freely available at https://github.com/mobiusklein/glycresoft in source form, with precompiled binaries for Windows.					
32223173	1	49	theme	incomplete	235:244	arg1	fragmentation					246:258	the incomplete fragmentation	231:258	the incomplete fragmentation of both moieties	231:275	Glycopeptides identified by tandem mass spectrometry rely on the identification of the peptide backbone sequence and the attached glycan(s) by the incomplete fragmentation of both moieties.					
32223173	2	50	theme	mass	463:466	arg1	determination					468:480	incorrect precursor mass determination	443:480	incorrect precursor mass determination	443:480	This may lead to ambiguous identifications where multiple structures could explain the same spectrum equally well due to missing information in the mass spectrum or incorrect precursor mass determination.					
32223173	6	51	theme	precompiled	1227:1237	arg1	binaries					1239:1246	precompiled binaries	1227:1246	precompiled binaries for Windows	1227:1258	We make this technique available as a command line executable program, written in Python and C, freely available at https://github.com/mobiusklein/glycresoft in source form, with precompiled binaries for Windows.					
33908010	5	0	theme	automation	723:732	arg1	platform					734:741	automation platform	723:741	automation platform	723:741	With the addition of automation platform, up to 96 N-glycan samples can be prepared and analyzed in a high-throughput manner.					
33908010	2	1	theme	structural	346:355	arg1	information					357:367	additional structural information	335:367	additional structural information	335:367	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	0	2	theme	Mass	77:80	arg1	Detection					82:90	Routine Mass Detection	69:90	Routine Mass Detection	69:90	Routine Analysis of N-Glycans Using Liquid Chromatography Coupled to Routine Mass Detection.					
33908010	1	3	theme	labeling	161:168	arg1	separation					202:211	enzymatic release, labeling, and liquid chromatography (LC) separation	142:211	enzymatic release, labeling, and liquid chromatography (LC) separation	142:211	Analysis of N-glycans are commonly conducted via enzymatic release, labeling, and liquid chromatography (LC) separation and fluorescent detection.					
33908010	3	4	with	generation	575:584	arg1	signal-enhancement					538:555	signal-enhancement	538:555	signal-enhancement	538:555	In this chapter, we describe a method to perform routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis.					
33908010	2	5	theme	additional	335:344	arg1	information					357:367	additional structural information	335:367	additional structural information	335:367	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	3	6	theme	LC-MS	564:568	arg1	data					570:573	LC-MS data	564:573	LC-MS data generation	564:584	In this chapter, we describe a method to perform routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis.					
33908010	3	7	theme	N-glycans	488:496	arg1	analysis					476:483	routine analysis	468:483	routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis	468:603	In this chapter, we describe a method to perform routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis.					
33908010	3	8	theme	routine	468:474	arg1	analysis					476:483	routine analysis	468:483	routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis	468:603	In this chapter, we describe a method to perform routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis.					
33908010	4	9	theme	N-glycan	634:641	arg1	samples					643:649	up to 24 N-glycan samples	625:649	up to 24 N-glycan samples	625:649	Using this method, up to 24 N-glycan samples can be prepared at one time and analyzed by LC-MS.					
33908010	3	10	with	preparation	519:529	arg1	signal-enhancement					538:555	signal-enhancement	538:555	signal-enhancement	538:555	In this chapter, we describe a method to perform routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis.					
33908010	5	11	theme	high-throughput	804:818	arg1	manner					820:825	a high-throughput manner	802:825	a high-throughput manner	802:825	With the addition of automation platform, up to 96 N-glycan samples can be prepared and analyzed in a high-throughput manner.					
33908010	5	12	theme	N-glycan	753:760	arg1	samples					762:768	up to 96 N-glycan samples	744:768	up to 96 N-glycan samples	744:768	With the addition of automation platform, up to 96 N-glycan samples can be prepared and analyzed in a high-throughput manner.					
33908010	5	13	theme	platform	734:741	arg1	addition					711:718	the addition	707:718	the addition of automation platform	707:741	With the addition of automation platform, up to 96 N-glycan samples can be prepared and analyzed in a high-throughput manner.					
33908010	3	14	with	analysis	596:603	arg1	signal-enhancement					538:555	signal-enhancement	538:555	signal-enhancement	538:555	In this chapter, we describe a method to perform routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis.					
33908010	2	15	theme	orthogonal	296:305	arg1	spectrometry					245:256	Mass spectrometry	240:256	Mass spectrometry (MS)	240:261	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	2	15	theme	orthogonal	296:305	arg1	method					317:322	an orthogonal detection method	293:322	an orthogonal detection method	293:322	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	1	16	theme	liquid	175:180	arg1	LC					198:199	LC	198:199	LC	198:199	Analysis of N-glycans are commonly conducted via enzymatic release, labeling, and liquid chromatography (LC) separation and fluorescent detection.					
33908010	1	16	theme	liquid	175:180	arg1	chromatography					182:195	liquid chromatography	175:195	liquid chromatography (LC)	175:200	Analysis of N-glycans are commonly conducted via enzymatic release, labeling, and liquid chromatography (LC) separation and fluorescent detection.					
33908010	0	17	theme	Routine	0:6	arg1	Analysis					8:15	Routine Analysis	0:15	Routine Analysis of N-Glycans	0:28	Routine Analysis of N-Glycans Using Liquid Chromatography Coupled to Routine Mass Detection.					
33908010	3	18	theme	data	570:573	arg1	generation					575:584	LC-MS data generation	564:584	LC-MS data generation	564:584	In this chapter, we describe a method to perform routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis.					
33908010	1	19	theme	chromatography	182:195	arg1	separation					202:211	enzymatic release, labeling, and liquid chromatography (LC) separation	142:211	enzymatic release, labeling, and liquid chromatography (LC) separation	142:211	Analysis of N-glycans are commonly conducted via enzymatic release, labeling, and liquid chromatography (LC) separation and fluorescent detection.					
33908010	0	20	theme	N-Glycans	20:28	arg1	Analysis					8:15	Routine Analysis	0:15	Routine Analysis of N-Glycans	0:28	Routine Analysis of N-Glycans Using Liquid Chromatography Coupled to Routine Mass Detection.					
33908010	1	21	theme	N-glycans	105:113	arg1	Analysis					93:100	Analysis	93:100	Analysis of N-glycans	93:113	Analysis of N-glycans are commonly conducted via enzymatic release, labeling, and liquid chromatography (LC) separation and fluorescent detection.					
33908010	5	22	dep	96	750:751	arg1	to					747:748	to	747:748	to	747:748	With the addition of automation platform, up to 96 N-glycan samples can be prepared and analyzed in a high-throughput manner.					
33908010	0	23	theme	Liquid	36:41	arg1	Chromatography					43:56	Liquid Chromatography	36:56	Liquid Chromatography Coupled to Routine Mass Detection	36:90	Routine Analysis of N-Glycans Using Liquid Chromatography Coupled to Routine Mass Detection.					
33908010	2	24	theme	analysis	409:416	arg1	confidence					386:395	the confidence	382:395	the confidence of N-glycan analysis	382:416	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	3	25	theme	data	591:594	arg1	analysis					596:603	data analysis	591:603	data analysis	591:603	In this chapter, we describe a method to perform routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis.					
33908010	2	26	theme	N-glycan	400:407	arg1	analysis					409:416	N-glycan analysis	400:416	N-glycan analysis	400:416	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	3	27	dep	signal-enhancement	538:555	arg1	label					557:561	label	557:561	label	557:561	In this chapter, we describe a method to perform routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis.					
33908010	1	28	theme	release	152:158	arg1	separation					202:211	enzymatic release, labeling, and liquid chromatography (LC) separation	142:211	enzymatic release, labeling, and liquid chromatography (LC) separation	142:211	Analysis of N-glycans are commonly conducted via enzymatic release, labeling, and liquid chromatography (LC) separation and fluorescent detection.					
33908010	2	29	used	used	285:288	arg2	MS					259:260	MS	259:260	MS	259:260	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	2	29	used	used	285:288	arg2	spectrometry					245:256	Mass spectrometry	240:256	Mass spectrometry (MS)	240:261	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	2	29	used	used	285:288	arg2	method					317:322	an orthogonal detection method	293:322	an orthogonal detection method	293:322	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	4	30	dep	24	631:632	arg1	to					628:629	to	628:629	to	628:629	Using this method, up to 24 N-glycan samples can be prepared at one time and analyzed by LC-MS.					
33908010	2	31	theme	detection	307:315	arg1	spectrometry					245:256	Mass spectrometry	240:256	Mass spectrometry (MS)	240:261	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	2	31	theme	detection	307:315	arg1	method					317:322	an orthogonal detection method	293:322	an orthogonal detection method	293:322	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	1	32	theme	fluorescent	217:227	arg1	detection					229:237	fluorescent detection	217:237	fluorescent detection	217:237	Analysis of N-glycans are commonly conducted via enzymatic release, labeling, and liquid chromatography (LC) separation and fluorescent detection.					
33908010	0	33	theme	Routine	69:75	arg1	Detection					82:90	Routine Mass Detection	69:90	Routine Mass Detection	69:90	Routine Analysis of N-Glycans Using Liquid Chromatography Coupled to Routine Mass Detection.					
33908010	1	34	theme	enzymatic	142:150	arg1	separation					202:211	enzymatic release, labeling, and liquid chromatography (LC) separation	142:211	enzymatic release, labeling, and liquid chromatography (LC) separation	142:211	Analysis of N-glycans are commonly conducted via enzymatic release, labeling, and liquid chromatography (LC) separation and fluorescent detection.					
33908010	2	35	theme	Mass	240:243	arg1	spectrometry					245:256	Mass spectrometry	240:256	Mass spectrometry (MS)	240:261	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	2	35	theme	Mass	240:243	arg1	method					317:322	an orthogonal detection method	293:322	an orthogonal detection method	293:322	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	2	35	theme	Mass	240:243	arg1	MS					259:260	MS	259:260	MS	259:260	Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide additional structural information and increase the confidence of N-glycan analysis.					
33908010	3	36	theme	sample	512:517	arg1	preparation					519:529	the sample preparation	508:529	the sample preparation with a signal-enhancement label	508:561	In this chapter, we describe a method to perform routine analysis of N-glycans including the sample preparation with a signal-enhancement label, LC-MS data generation, and data analysis.					
32929138	0	0	theme	immune	80:85	arg1	recognition					87:97	immune recognition	80:97	immune recognition	80:97	Analysis of the SARS-CoV-2 spike protein glycan shield reveals implications for immune recognition.					
32929138	5	1	theme	protein	1022:1028	arg1	surface					1030:1036	the protein surface	1018:1036	the protein surface	1018:1036	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	6	2	theme	modest	1275:1280	arg1	contribution					1282:1293	the relatively modest contribution	1260:1293	the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform)	1260:1385	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	5	3	theme	notable	1109:1115	arg1	exception					1117:1125	the notable exception	1105:1125	the notable exception of the ACE2 receptor binding domain	1105:1161	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	2	4	theme	nascent	367:373	arg1	glycoproteins					375:387	the nascent glycoproteins	363:387	the nascent glycoproteins (prior to enzymatic modifications in the Golgi)	363:435	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	4	5	theme	antigen	802:808	arg1	complexes					816:824	human leukocyte antigen (HLA) complexes	786:824	human leukocyte antigen (HLA) complexes	786:824	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	2	6	from	viruses	511:517	arg1	present					494:500	present	494:500	present	494:500	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	4	7	theme	glycosylation	861:873	arg1	role					843:846	the role	839:846	the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine	839:987	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	2	8	gly	glycoproteins	375:387	arg1	glycoproteins					375:387	the nascent glycoproteins	363:387	the nascent glycoproteins (prior to enzymatic modifications in the Golgi)	363:435	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	4	9	from	peptides	720:727	arg1	glycoprotein					738:749	the S glycoprotein	732:749	the S glycoprotein	732:749	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	4	10	theme	related	973:979	arg1	vaccine					981:987	a related vaccine	971:987	a related vaccine	971:987	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	1	11	theme	glycomics	234:242	arg1	data					244:247	glycomics data	234:247	glycomics data	234:247	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	4	12	theme	protein	853:859	arg1	glycosylation					861:873	S protein glycosylation	851:873	S protein glycosylation	851:873	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	3	13	theme	S	677:677	arg1	glycoprotein					679:690	the S glycoprotein	673:690	the S glycoprotein	673:690	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.					
32929138	3	14	theme	glycoprotein	679:690	arg1	antigenicity					657:668	the antigenicity	653:668	the antigenicity of the S glycoprotein	653:690	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.					
32929138	1	15	from	glycoforms	140:149	arg1	SARS-CoV-2					186:195	SARS-CoV-2	186:195	SARS-CoV-2	186:195	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	6	16	theme	protein	1424:1430	arg1	surface					1432:1438	the protein surface	1420:1438	the protein surface	1420:1438	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	5	17	theme	specific	1232:1239	arg1	glycoform					1241:1249	the specific glycoform	1228:1249	the specific glycoform	1228:1249	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	1	18	theme	spike	158:162	arg1	glycoprotein					168:179	the spike (S) glycoprotein	154:179	the spike (S) glycoprotein from SARS-CoV-2	154:195	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	1	19	gly	glycoprotein	168:179	arg1	glycoprotein					168:179	the spike (S) glycoprotein	154:179	the spike (S) glycoprotein from SARS-CoV-2	154:195	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	6	20	theme	molecular	1323:1331	arg1	weight					1333:1338	the total molecular weight	1313:1338	the total molecular weight of the S trimer (17% for the HEK293 glycoform)	1313:1385	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	4	21	theme	SARS-CoV-2	948:957	arg1	virus					959:963	the SARS-CoV-2 virus	944:963	the SARS-CoV-2 virus	944:963	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	4	22	theme	innate	905:910	arg1	response					932:939	the innate and adaptive immune response	901:939	the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine	901:987	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	4	23	theme	leukocyte	792:800	arg1	HLA					811:813	HLA	811:813	HLA	811:813	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	4	23	theme	leukocyte	792:800	arg1	antigen					802:808	human leukocyte antigen	786:808	human leukocyte antigen (HLA) complexes	786:824	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	6	24	theme	glycans	1302:1308	arg1	contribution					1282:1293	the relatively modest contribution	1260:1293	the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform)	1260:1385	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	6	25	gly	glycoform	1376:1384	arg1	HEK293					1369:1374	the HEK293 glycoform	1365:1384	the HEK293 glycoform	1365:1384	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	1	26	theme	S	165:165	arg1	glycoprotein					168:179	the spike (S) glycoprotein	154:179	the spike (S) glycoprotein from SARS-CoV-2	154:195	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	4	27	theme	S	851:851	arg1	glycosylation					861:873	S protein glycosylation	851:873	S protein glycosylation	851:873	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	3	28	gly	glycoprotein	679:690	arg1	glycoprotein					679:690	the S glycoprotein	673:690	the S glycoprotein	673:690	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.					
32929138	4	29	theme	human	786:790	arg1	HLA					811:813	HLA	811:813	HLA	811:813	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	4	29	theme	human	786:790	arg1	antigen					802:808	human leukocyte antigen	786:808	human leukocyte antigen (HLA) complexes	786:824	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	0	30	theme	SARS-CoV-2	16:25	arg1	protein					33:39	the SARS-CoV-2 spike protein glycan shield	12:53	the SARS-CoV-2 spike protein glycan shield	12:53	Analysis of the SARS-CoV-2 spike protein glycan shield reveals implications for immune recognition.					
32929138	2	31	theme	other	505:509	arg1	viruses					511:517	other viruses	505:517	other viruses	505:517	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	1	32	theme	glycoprotein	168:179	arg1	glycoforms					140:149	glycoforms	140:149	glycoforms of the spike (S) glycoprotein from SARS-CoV-2	140:195	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	2	33	theme	enzymatic	399:407	arg1	modifications					409:421	enzymatic modifications	399:421	enzymatic modifications in the Golgi	399:434	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	6	34	theme	total	1317:1321	arg1	weight					1333:1338	the total molecular weight	1313:1338	the total molecular weight of the S trimer (17% for the HEK293 glycoform)	1313:1385	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	2	35	theme	prior	390:394	arg1	glycoproteins					375:387	the nascent glycoproteins	363:387	the nascent glycoproteins (prior to enzymatic modifications in the Golgi)	363:435	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	0	36	theme	protein	33:39	arg1	Analysis					0:7	Analysis	0:7	Analysis of the SARS-CoV-2 spike protein glycan shield	0:53	Analysis of the SARS-CoV-2 spike protein glycan shield reveals implications for immune recognition.					
32929138	2	37	theme	present	494:500	arg1	antigens					485:492	antigens	485:492	antigens present in other viruses	485:517	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	5	38	theme	ACE2	1134:1137	arg1	domain					1156:1161	the ACE2 receptor binding domain	1130:1161	the ACE2 receptor binding domain	1130:1161	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	6	39	theme	S	1347:1347	arg1	trimer					1349:1354	the S trimer	1343:1354	the S trimer	1343:1354	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	2	40	attach	present	494:500	arg2	antigens					485:492	antigens	485:492	antigens present in other viruses	485:517	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	2	40	attach	present	494:500	arg1	viruses					511:517	other viruses	505:517	other viruses	505:517	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	4	41	dep	likely	760:765	arg1	presented					773:781	presented	773:781	to be presented in human leukocyte antigen (HLA) complexes	767:824	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	4	41	dep	likely	760:765	arg1	discuss					831:837	discuss	831:837	discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine	831:987	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	0	42	theme	spike	27:31	arg1	protein					33:39	the SARS-CoV-2 spike protein glycan shield	12:53	the SARS-CoV-2 spike protein glycan shield	12:53	Analysis of the SARS-CoV-2 spike protein glycan shield reveals implications for immune recognition.					
32929138	4	43	theme	adaptive	916:923	arg1	response					932:939	the innate and adaptive immune response	901:939	the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine	901:987	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	5	44	theme	3D	994:995	arg1	structures					997:1006	The 3D structures	990:1006	The 3D structures	990:1006	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	3	45	theme	molecular	551:559	arg1	MD					571:572	MD	571:572	MD	571:572	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.					
32929138	3	45	theme	molecular	551:559	arg1	dynamics					561:568	molecular dynamics	551:568	molecular dynamics (MD) simulation	551:584	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.					
32929138	2	46	located	observed	473:480	arg1	antigens					485:492	antigens	485:492	antigens present in other viruses	485:517	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	2	46	located	observed	473:480	arg2	those					449:453	those	449:453	those	449:453	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	0	47	theme	shield	48:53	arg1	protein					33:39	the SARS-CoV-2 spike protein glycan shield	12:53	the SARS-CoV-2 spike protein glycan shield	12:53	Analysis of the SARS-CoV-2 spike protein glycan shield reveals implications for immune recognition.					
32929138	1	48	theme	HEK293	277:282	arg1	cells					284:288	HEK293 cells	277:288	HEK293 cells	277:288	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	1	49	gly	glycoforms	140:149	arg1	glycoprotein					168:179	the spike (S) glycoprotein	154:179	the spike (S) glycoprotein from SARS-CoV-2	154:195	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	2	50	from	present	352:358	arg1	glycoproteins					375:387	the nascent glycoproteins	363:387	the nascent glycoproteins (prior to enzymatic modifications in the Golgi)	363:435	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	0	51	theme	glycan	41:46	arg1	protein					33:39	the SARS-CoV-2 spike protein glycan shield	12:53	the SARS-CoV-2 spike protein glycan shield	12:53	Analysis of the SARS-CoV-2 spike protein glycan shield reveals implications for immune recognition.					
32929138	6	52	dep	weight	1333:1338	arg1	%					1359:1359	17%	1357:1359	17% for the HEK293 glycoform	1357:1384	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	5	53	theme	binding	1148:1154	arg1	domain					1156:1161	the ACE2 receptor binding domain	1130:1161	the ACE2 receptor binding domain	1130:1161	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	3	54	theme	glycan	619:624	arg1	microheterogeneity					626:643	glycan microheterogeneity	619:643	glycan microheterogeneity	619:643	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.					
32929138	4	55	theme	S	736:736	arg1	glycoprotein					738:749	the S glycoprotein	732:749	the S glycoprotein	732:749	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	1	56	theme	3D	123:124	arg1	structures					126:135	3D structures	123:135	3D structures	123:135	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	6	57	theme	HEK293	1369:1374	arg1	glycoform					1376:1384	the HEK293 glycoform	1365:1384	the HEK293 glycoform	1365:1384	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	2	58	from	present	494:500	arg1	viruses					511:517	other viruses	505:517	other viruses	505:517	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	4	59	theme	immune	925:930	arg1	response					932:939	the innate and adaptive immune response	901:939	the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine	901:987	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	2	60	attach	present	352:358	arg2	those					346:350	those	346:350	those	346:350	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	2	60	attach	present	352:358	arg1	glycoproteins					375:387	the nascent glycoproteins	363:387	the nascent glycoproteins (prior to enzymatic modifications in the Golgi)	363:435	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	1	61	from	SARS-CoV-2	186:195	arg1	glycoforms					140:149	glycoforms	140:149	glycoforms of the spike (S) glycoprotein from SARS-CoV-2	140:195	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	1	61	from	SARS-CoV-2	186:195	arg1	glycoprotein					168:179	the spike (S) glycoprotein	154:179	the spike (S) glycoprotein from SARS-CoV-2	154:195	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	5	62	theme	receptor	1139:1146	arg1	domain					1156:1161	the ACE2 receptor binding domain	1130:1161	the ACE2 receptor binding domain	1130:1161	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	5	63	theme	antibody	1067:1074	arg1	recognition					1076:1086	antibody recognition	1067:1086	antibody recognition by glycans	1067:1097	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	6	64	theme	trimer	1349:1354	arg1	weight					1333:1338	the total molecular weight	1313:1338	the total molecular weight of the S trimer (17% for the HEK293 glycoform)	1313:1385	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	3	65	theme	dynamics	561:568	arg1	simulation					575:584	molecular dynamics (MD) simulation	551:584	molecular dynamics (MD) simulation	551:584	These models were subjected to molecular dynamics (MD) simulation to determine the extent to which glycan microheterogeneity impacts the antigenicity of the S glycoprotein.					
32929138	2	66	from	glycoproteins	375:387	arg1	present					352:358	present	352:358	present	352:358	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	5	67	theme	shielding	1192:1200	arg1	insensitive					1213:1223	insensitive	1213:1223	insensitive	1213:1223	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	5	67	theme	shielding	1192:1200	arg1	degree					1182:1187	the degree	1178:1187	the degree of shielding	1178:1200	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	1	68	theme	reported	207:214	arg1	structures					219:228	reported 3D structures	207:228	reported 3D structures	207:228	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	4	69	gly	glycoprotein	738:749	arg1	glycoprotein					738:749	the S glycoprotein	732:749	the S glycoprotein	732:749	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	2	70	from	modifications	409:421	arg1	Golgi					430:434	the Golgi	426:434	the Golgi	426:434	We also analyze structures for glycoforms representing those present in the nascent glycoproteins (prior to enzymatic modifications in the Golgi), as well as those that are commonly observed on antigens present in other viruses.					
32929138	5	71	theme	domain	1156:1161	arg1	exception					1117:1125	the notable exception	1105:1125	the notable exception of the ACE2 receptor binding domain	1105:1161	The 3D structures show that the protein surface is extensively shielded from antibody recognition by glycans, with the notable exception of the ACE2 receptor binding domain, and also that the degree of shielding is largely insensitive to the specific glycoform.					
32929138	6	72	theme	surface	1432:1438	arg1	%					1415:1415	approximately 40%	1399:1415	approximately 40% of the protein surface	1399:1438	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	6	72	theme	surface	1432:1438	arg1	surface					1432:1438	the protein surface	1420:1438	the protein surface	1420:1438	Despite the relatively modest contribution of the glycans to the total molecular weight of the S trimer (17% for the HEK293 glycoform) they shield approximately 40% of the protein surface.					
32929138	1	73	theme	glycoforms	140:149	arg1	structures					126:135	3D structures	123:135	3D structures	123:135	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32929138	4	74	attach	presented	773:781	arg1	complexes					816:824	human leukocyte antigen (HLA) complexes	786:824	human leukocyte antigen (HLA) complexes	786:824	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	4	74	attach	presented	773:781	arg2	peptides					720:727	peptides	720:727	peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine	720:987	Lastly, we have identified peptides in the S glycoprotein that are likely to be presented in human leukocyte antigen (HLA) complexes, and discuss the role of S protein glycosylation in potentially modulating the innate and adaptive immune response to the SARS-CoV-2 virus or to a related vaccine.					
32929138	1	75	theme	3D	216:217	arg1	structures					219:228	reported 3D structures	207:228	reported 3D structures	207:228	Here we have generated 3D structures of glycoforms of the spike (S) glycoprotein from SARS-CoV-2, based on reported 3D structures and glycomics data for the protein produced in HEK293 cells.					
32817216	10	0	theme	small-molecule	2454:2467	arg1	inhibitors					2475:2484	small-molecule viral inhibitors	2454:2484	small-molecule viral inhibitors that allosterically block CD4 binding	2454:2522	The use of small-molecule viral inhibitors that allosterically block CD4 binding represents an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers.					
32817216	4	1	theme	limited	972:978	arg1	exposure					980:987	limited exposure	972:987	limited exposure of CD4i epitopes	972:1004	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	11	2	theme	immunogens	2824:2833	arg1	immunogenicity					2788:2801	the immunogenicity	2784:2801	the immunogenicity of select Env trimer immunogens	2784:2833	In this study, we report that the viral entry inhibitor BMS-626529 restricts trimer conformational transition and improves the immunogenicity of select Env trimer immunogens.					
32817216	3	3	theme	unmutated	659:667	arg1	ancestor					676:683	their unmutated common ancestor	653:683	their unmutated common ancestor (UCA) antibodies	653:700	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	3	3	theme	unmutated	659:667	arg1	UCA					686:688	UCA	686:688	UCA	686:688	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	8	4	theme	neutralizing	1926:1937	arg1	antibodies					1939:1948	tier 2 neutralizing antibodies	1919:1948	tier 2 neutralizing antibodies of higher potency	1919:1966	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	6	5	theme	stronger	1451:1458	arg1	neutralization					1460:1473	stronger neutralization	1451:1473	stronger neutralization	1451:1473	In rhesus macaques, BMS-529 complexed to CD4 bs-targeting ch.SOSIP immunogen induced stronger neutralization against tier 2 pseudoviruses bearing high-mannose glycans than noncomplexed ch.SOSIP trimer immunogen.					
32817216	4	6	theme	CHO	853:855	arg1	cells					857:861	CHO cells	853:861	CHO cells	853:861	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	11	7	theme	Env	2813:2815	arg1	immunogens					2824:2833	select Env trimer immunogens	2806:2833	select Env trimer immunogens	2806:2833	In this study, we report that the viral entry inhibitor BMS-626529 restricts trimer conformational transition and improves the immunogenicity of select Env trimer immunogens.					
32817216	8	8	theme	trimer	1812:1817	arg1	conformation					1819:1830	Env trimer conformation	1808:1830	Env trimer conformation	1808:1830	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	1	9	theme	CD4-induced	249:259	arg1	changes					272:278	CD4-induced structural changes	249:278	CD4-induced structural changes in Env trimers	249:293	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	9	10	theme	recent	2291:2296	arg1	approaches					2315:2324	recent structure-guided approaches	2291:2324	recent structure-guided approaches	2291:2324	These limitations have been mitigated through recent structure-guided approaches and include trimer-stabilizing mutations that resist trimer conformational transition and exposure of CD4i epitopes.					
32817216	8	11	theme	potency	1960:1966	arg1	antibodies					1939:1948	tier 2 neutralizing antibodies	1919:1948	tier 2 neutralizing antibodies of higher potency	1919:1966	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	2	12	theme	membrane-bound	393:406	arg1	trimers					408:414	membrane-bound trimers	393:414	membrane-bound trimers with intact transmembrane domain (gp150)	393:455	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	6	13	theme	trimer	1560:1565	arg1	immunogen					1567:1575	noncomplexed ch.SOSIP trimer immunogen	1538:1575	noncomplexed ch.SOSIP trimer immunogen	1538:1575	In rhesus macaques, BMS-529 complexed to CD4 bs-targeting ch.SOSIP immunogen induced stronger neutralization against tier 2 pseudoviruses bearing high-mannose glycans than noncomplexed ch.SOSIP trimer immunogen.					
32817216	10	14	theme	inhibitors	2475:2484	arg1	use					2447:2449	The use	2443:2449	The use of small-molecule viral inhibitors that allosterically block CD4 binding	2443:2522	The use of small-molecule viral inhibitors that allosterically block CD4 binding represents an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers.					
32817216	1	15	theme	structural	261:270	arg1	changes					272:278	CD4-induced structural changes	249:278	CD4-induced structural changes in Env trimers	249:293	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	4	16	theme	glycans	1071:1077	arg1	preponderance					1041:1053	a preponderance	1039:1053	a preponderance of high-mannose glycans	1039:1077	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	1	17	from	changes	272:278	arg1	trimers					287:293	Env trimers	283:293	Env trimers	283:293	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	6	18	theme	noncomplexed	1538:1549	arg1	immunogen					1567:1575	noncomplexed ch.SOSIP trimer immunogen	1538:1575	noncomplexed ch.SOSIP trimer immunogen	1538:1575	In rhesus macaques, BMS-529 complexed to CD4 bs-targeting ch.SOSIP immunogen induced stronger neutralization against tier 2 pseudoviruses bearing high-mannose glycans than noncomplexed ch.SOSIP trimer immunogen.					
32817216	8	19	theme	Soluble	2005:2011	arg1	forms					2013:2017	noncomplexed trimers.IMPORTANCE Soluble forms	1973:2017	noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers	1973:2043	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	8	20	theme	Env	1808:1810	arg1	conformation					1819:1830	Env trimer conformation	1808:1830	Env trimer conformation	1808:1830	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	1	21	theme	CD4	201:203	arg1	binding					205:211	CD4 binding	201:211	CD4 binding to HIV-1 envelope (Env)	201:235	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	7	22	theme	tier	1672:1675	arg1	pseudoviruses					1679:1691	tier 2 pseudoviruses	1672:1691	tier 2 pseudoviruses	1672:1691	When immunized with gp150 complexed to BMS-529, rhesus macaques showed neutralization against tier 2 pseudoviruses with targeted glycan deletion and high-mannose glycan enrichment.					
32817216	10	23	theme	CD4	2512:2514	arg1	binding					2516:2522	CD4 binding	2512:2522	CD4 binding	2512:2522	The use of small-molecule viral inhibitors that allosterically block CD4 binding represents an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers.					
32817216	11	24	theme	trimer	2738:2743	arg1	transition					2760:2769	trimer conformational transition	2738:2769	trimer conformational transition	2738:2769	In this study, we report that the viral entry inhibitor BMS-626529 restricts trimer conformational transition and improves the immunogenicity of select Env trimer immunogens.					
32817216	8	25	theme	non-neutralizing	2137:2152	arg1	antibodies					2154:2163	non-neutralizing antibodies	2137:2163	non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses	2137:2242	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	8	26	theme	conformation	1819:1830	arg1	stabilization					1791:1803	stabilization	1791:1803	stabilization of Env trimer conformation with BMS-529	1791:1843	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	8	27	theme	envelope	2028:2035	arg1	trimers					2037:2043	HIV-1 envelope trimers	2022:2043	HIV-1 envelope trimers	2022:2043	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	3	28	theme	ch.SOSIP	563:570	arg1	trimers					572:578	ch.SOSIP trimers	563:578	ch.SOSIP trimers	563:578	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	2	29	with	trimers	408:414	arg1	gp150					450:454	gp150	450:454	gp150	450:454	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	2	29	with	trimers	408:414	arg1	domain					442:447	intact transmembrane domain	421:447	intact transmembrane domain (gp150)	421:455	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	1	30	theme	entry	132:136	arg1	inhibitors					138:147	Small-molecule viral entry inhibitors	111:147	Small-molecule viral entry inhibitors	111:147	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	1	30	theme	entry	132:136	arg1	BMS-626529					158:167	BMS-626529	158:167	BMS-626529	158:167	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	10	31	theme	alternative	2538:2548	arg1	strategy					2550:2557	an alternative strategy	2535:2557	an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers	2535:2658	The use of small-molecule viral inhibitors that allosterically block CD4 binding represents an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers.					
32817216	9	32	theme	conformational	2386:2399	arg1	transition					2401:2410	trimer conformational transition	2379:2410	trimer conformational transition	2379:2410	These limitations have been mitigated through recent structure-guided approaches and include trimer-stabilizing mutations that resist trimer conformational transition and exposure of CD4i epitopes.					
32817216	10	33	theme	viral	2469:2473	arg1	inhibitors					2475:2484	small-molecule viral inhibitors	2454:2484	small-molecule viral inhibitors that allosterically block CD4 binding	2454:2522	The use of small-molecule viral inhibitors that allosterically block CD4 binding represents an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers.					
32817216	4	34	theme	binding	923:929	arg1	bs					937:938	bs	937:938	bs	937:938	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	4	34	theme	binding	923:929	arg1	site					931:934	CD4 binding site	919:934	the CD4 binding site (bs) CH103 bNAb lineage	915:958	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	3	35	theme	epitopes	749:756	arg1	exposure					709:716	exposure	709:716	exposure of CD4-induced (CD4i) non-bNAb epitopes	709:756	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	6	36	theme	rhesus	1369:1374	arg1	macaques					1376:1383	rhesus macaques	1369:1383	rhesus macaques	1369:1383	In rhesus macaques, BMS-529 complexed to CD4 bs-targeting ch.SOSIP immunogen induced stronger neutralization against tier 2 pseudoviruses bearing high-mannose glycans than noncomplexed ch.SOSIP trimer immunogen.					
32817216	10	37	theme	Env	2575:2577	arg1	trimer					2579:2584	Env trimer	2575:2584	Env trimer	2575:2584	The use of small-molecule viral inhibitors that allosterically block CD4 binding represents an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers.					
32817216	0	38	theme	Small-Molecule	73:86	arg1	Inhibitor					100:108	a Small-Molecule Viral Entry Inhibitor	71:108	a Small-Molecule Viral Entry Inhibitor	71:108	Antigenicity and Immunogenicity of HIV-1 Envelope Trimers Complexed to a Small-Molecule Viral Entry Inhibitor.					
32817216	8	39	theme	chimeric	1883:1890	arg1	trimers					1898:1904	select chimeric SOSIP trimers	1876:1904	select chimeric SOSIP trimers	1876:1904	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	9	40	theme	epitopes	2433:2440	arg1	exposure					2416:2423	exposure	2416:2423	exposure of CD4i epitopes	2416:2440	These limitations have been mitigated through recent structure-guided approaches and include trimer-stabilizing mutations that resist trimer conformational transition and exposure of CD4i epitopes.					
32817216	9	40	theme	epitopes	2433:2440	arg1	transition					2401:2410	trimer conformational transition	2379:2410	trimer conformational transition	2379:2410	These limitations have been mitigated through recent structure-guided approaches and include trimer-stabilizing mutations that resist trimer conformational transition and exposure of CD4i epitopes.					
32817216	0	41	theme	Entry	94:98	arg1	Inhibitor					100:108	a Small-Molecule Viral Entry Inhibitor	71:108	a Small-Molecule Viral Entry Inhibitor	71:108	Antigenicity and Immunogenicity of HIV-1 Envelope Trimers Complexed to a Small-Molecule Viral Entry Inhibitor.					
32817216	8	42	theme	trimers	1898:1904	arg1	immunogenicity					1858:1871	the immunogenicity	1854:1871	the immunogenicity of select chimeric SOSIP trimers	1854:1904	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	7	43	theme	glycan	1740:1745	arg1	enrichment					1747:1756	high-mannose glycan enrichment	1727:1756	high-mannose glycan enrichment	1727:1756	When immunized with gp150 complexed to BMS-529, rhesus macaques showed neutralization against tier 2 pseudoviruses with targeted glycan deletion and high-mannose glycan enrichment.					
32817216	2	44	theme	conformational	474:487	arg1	transitions					489:499	trimer conformational transitions	467:499	trimer conformational transitions	467:499	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	11	45	theme	entry	2701:2705	arg1	BMS-626529					2717:2726	the viral entry inhibitor BMS-626529	2691:2726	the viral entry inhibitor BMS-626529	2691:2726	In this study, we report that the viral entry inhibitor BMS-626529 restricts trimer conformational transition and improves the immunogenicity of select Env trimer immunogens.					
32817216	2	46	theme	chimeric	357:364	arg1	SOSIP					372:376	clade C soluble chimeric gp140 SOSIP	341:376	clade C soluble chimeric gp140 SOSIP (ch.SOSIP)	341:387	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	2	46	theme	chimeric	357:364	arg1	ch.SOSIP					379:386	ch.SOSIP	379:386	ch.SOSIP	379:386	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	10	47	theme	membrane-bound	2637:2650	arg1	trimers					2652:2658	both soluble and membrane-bound trimers	2620:2658	both soluble and membrane-bound trimers	2620:2658	The use of small-molecule viral inhibitors that allosterically block CD4 binding represents an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers.					
32817216	8	48	with	stabilization	1791:1803	arg1	BMS-529					1837:1843	BMS-529	1837:1843	BMS-529	1837:1843	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	2	49	theme	C	347:347	arg1	SOSIP					372:376	clade C soluble chimeric gp140 SOSIP	341:376	clade C soluble chimeric gp140 SOSIP (ch.SOSIP)	341:387	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	2	49	theme	C	347:347	arg1	ch.SOSIP					379:386	ch.SOSIP	379:386	ch.SOSIP	379:386	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	6	50	theme	ch.SOSIP	1424:1431	arg1	immunogen					1433:1441	CD4 bs-targeting ch.SOSIP immunogen	1407:1441	CD4 bs-targeting ch.SOSIP immunogen	1407:1441	In rhesus macaques, BMS-529 complexed to CD4 bs-targeting ch.SOSIP immunogen induced stronger neutralization against tier 2 pseudoviruses bearing high-mannose glycans than noncomplexed ch.SOSIP trimer immunogen.					
32817216	5	51	theme	V3	1110:1111	arg1	immunogen					1139:1147	BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen	1092:1147	BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen	1092:1147	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	7	52	theme	glycan	1707:1712	arg1	deletion					1714:1721	targeted glycan deletion	1698:1721	targeted glycan deletion	1698:1721	When immunized with gp150 complexed to BMS-529, rhesus macaques showed neutralization against tier 2 pseudoviruses with targeted glycan deletion and high-mannose glycan enrichment.					
32817216	10	53	theme	soluble	2625:2631	arg1	trimers					2652:2658	both soluble and membrane-bound trimers	2620:2658	both soluble and membrane-bound trimers	2620:2658	The use of small-molecule viral inhibitors that allosterically block CD4 binding represents an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers.					
32817216	5	54	theme	heterologous	1279:1290	arg1	pseudoviruses					1299:1311	both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses	1231:1311	both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses	1231:1311	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	6	55	theme	CD4	1407:1409	arg1	immunogen					1433:1441	CD4 bs-targeting ch.SOSIP immunogen	1407:1441	CD4 bs-targeting ch.SOSIP immunogen	1407:1441	In rhesus macaques, BMS-529 complexed to CD4 bs-targeting ch.SOSIP immunogen induced stronger neutralization against tier 2 pseudoviruses bearing high-mannose glycans than noncomplexed ch.SOSIP trimer immunogen.					
32817216	5	56	theme	ch.SOSIP	1130:1137	arg1	immunogen					1139:1147	BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen	1092:1147	BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen	1092:1147	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	1	57	theme	Small-molecule	111:124	arg1	inhibitors					138:147	Small-molecule viral entry inhibitors	111:147	Small-molecule viral entry inhibitors	111:147	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	1	57	theme	Small-molecule	111:124	arg1	BMS-626529					158:167	BMS-626529	158:167	BMS-626529	158:167	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	8	58	theme	neutralizing	2212:2223	arg1	responses					2234:2242	broad neutralizing antibody responses	2206:2242	broad neutralizing antibody responses	2206:2242	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	4	59	theme	gp150	791:795	arg1	trimers					797:803	BMS-529-complexed gp150 trimers	773:803	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells,	773:862	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	0	60	theme	HIV-1	35:39	arg1	Trimers					50:56	HIV-1 Envelope Trimers	35:56	HIV-1 Envelope Trimers	35:56	Antigenicity and Immunogenicity of HIV-1 Envelope Trimers Complexed to a Small-Molecule Viral Entry Inhibitor.					
32817216	8	61	theme	antibodies	2154:2163	arg1	epitopes					2125:2132	epitopes	2125:2132	epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses	2125:2242	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	8	62	theme	responses	2234:2242	arg1	induction					2193:2201	induction	2193:2201	induction of broad neutralizing antibody responses	2193:2242	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	4	63	theme	bNAb	947:950	arg1	lineage					952:958	the CD4 binding site (bs) CH103 bNAb lineage	915:958	the CD4 binding site (bs) CH103 bNAb lineage	915:958	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	0	64	theme	Trimers	50:56	arg1	Immunogenicity					17:30	Immunogenicity	17:30	Immunogenicity	17:30	Antigenicity and Immunogenicity of HIV-1 Envelope Trimers Complexed to a Small-Molecule Viral Entry Inhibitor.					
32817216	0	64	theme	Trimers	50:56	arg1	Antigenicity					0:11	Antigenicity	0:11	Antigenicity	0:11	Antigenicity and Immunogenicity of HIV-1 Envelope Trimers Complexed to a Small-Molecule Viral Entry Inhibitor.					
32817216	1	65	theme	HIV-1	216:220	arg1	Env					232:234	Env	232:234	Env	232:234	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	1	65	theme	HIV-1	216:220	arg1	envelope					222:229	HIV-1 envelope	216:229	HIV-1 envelope (Env)	216:235	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	4	66	with	pattern	1026:1032	arg1	preponderance					1041:1053	a preponderance	1039:1053	a preponderance of high-mannose glycans	1039:1077	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	4	67	theme	glycosylation	1012:1024	arg1	pattern					1026:1032	a glycosylation pattern	1010:1032	a glycosylation pattern with a preponderance of high-mannose glycans	1010:1077	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	11	68	theme	select	2806:2811	arg1	immunogens					2824:2833	select Env trimer immunogens	2806:2833	select Env trimer immunogens	2806:2833	In this study, we report that the viral entry inhibitor BMS-626529 restricts trimer conformational transition and improves the immunogenicity of select Env trimer immunogens.					
32817216	10	69	theme	pre-CD4-triggered	2593:2609	arg1	state					2611:2615	the pre-CD4-triggered state	2589:2615	the pre-CD4-triggered state of both soluble and membrane-bound trimers	2589:2658	The use of small-molecule viral inhibitors that allosterically block CD4 binding represents an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers.					
32817216	6	70	theme	tier	1483:1486	arg1	pseudoviruses					1490:1502	tier 2 pseudoviruses	1483:1502	tier 2 pseudoviruses bearing high-mannose glycans	1483:1531	In rhesus macaques, BMS-529 complexed to CD4 bs-targeting ch.SOSIP immunogen induced stronger neutralization against tier 2 pseudoviruses bearing high-mannose glycans than noncomplexed ch.SOSIP trimer immunogen.					
32817216	5	71	theme	animals	1186:1192	arg1	majority					1164:1171	the majority	1160:1171	the majority of immunized animals	1160:1192	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	2	72	theme	intact	421:426	arg1	gp150					450:454	gp150	450:454	gp150	450:454	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	2	72	theme	intact	421:426	arg1	domain					442:447	intact transmembrane domain	421:447	intact transmembrane domain (gp150)	421:455	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	8	73	theme	CD4-induced	2094:2104	arg1	CD4i					2107:2110	CD4i	2107:2110	CD4i	2107:2110	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	8	73	theme	CD4-induced	2094:2104	arg1	exposure					2113:2120	CD4-induced (CD4i) exposure	2094:2120	CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses	2094:2242	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	4	74	theme	CD4i	992:995	arg1	epitopes					997:1004	CD4i epitopes	992:1004	CD4i epitopes	992:1004	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	3	75	theme	common	669:674	arg1	ancestor					676:683	their unmutated common ancestor	653:683	their unmutated common ancestor (UCA) antibodies	653:700	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	3	75	theme	common	669:674	arg1	UCA					686:688	UCA	686:688	UCA	686:688	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	8	76	theme	higher	1953:1958	arg1	potency					1960:1966	higher potency	1953:1966	higher potency	1953:1966	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	2	77	with	SOSIP	372:376	arg1	gp150					450:454	gp150	450:454	gp150	450:454	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	2	77	with	SOSIP	372:376	arg1	domain					442:447	intact transmembrane domain	421:447	intact transmembrane domain (gp150)	421:455	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	4	78	theme	high-mannose	1058:1069	arg1	glycans					1071:1077	high-mannose glycans	1058:1077	high-mannose glycans	1058:1077	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	11	79	theme	trimer	2817:2822	arg1	immunogens					2824:2833	select Env trimer immunogens	2806:2833	select Env trimer immunogens	2806:2833	In this study, we report that the viral entry inhibitor BMS-626529 restricts trimer conformational transition and improves the immunogenicity of select Env trimer immunogens.					
32817216	10	80	theme	trimers	2652:2658	arg1	state					2611:2615	the pre-CD4-triggered state	2589:2615	the pre-CD4-triggered state of both soluble and membrane-bound trimers	2589:2658	The use of small-molecule viral inhibitors that allosterically block CD4 binding represents an alternative strategy for stabilizing Env trimer in the pre-CD4-triggered state of both soluble and membrane-bound trimers.					
32817216	4	81	theme	detergent	808:816	arg1	micelles					818:825	detergent micelles	808:825	detergent micelles	808:825	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	9	82	theme	structure-guided	2298:2313	arg1	approaches					2315:2324	recent structure-guided approaches	2291:2324	recent structure-guided approaches	2291:2324	These limitations have been mitigated through recent structure-guided approaches and include trimer-stabilizing mutations that resist trimer conformational transition and exposure of CD4i epitopes.					
32817216	8	83	theme	trimers.IMPORTANCE	1986:2003	arg1	forms					2013:2017	noncomplexed trimers.IMPORTANCE Soluble forms	1973:2017	noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers	1973:2043	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	6	84	theme	ch.SOSIP	1551:1558	arg1	immunogen					1567:1575	noncomplexed ch.SOSIP trimer immunogen	1538:1575	noncomplexed ch.SOSIP trimer immunogen	1538:1575	In rhesus macaques, BMS-529 complexed to CD4 bs-targeting ch.SOSIP immunogen induced stronger neutralization against tier 2 pseudoviruses bearing high-mannose glycans than noncomplexed ch.SOSIP trimer immunogen.					
32817216	8	85	theme	noncomplexed	1973:1984	arg1	forms					2013:2017	noncomplexed trimers.IMPORTANCE Soluble forms	1973:2017	noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers	1973:2043	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	3	86	theme	CD4-induced	721:731	arg1	epitopes					749:756	CD4-induced (CD4i) non-bNAb epitopes	721:756	CD4-induced (CD4i) non-bNAb epitopes	721:756	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	9	87	theme	trimer-stabilizing	2338:2355	arg1	mutations					2357:2365	trimer-stabilizing mutations	2338:2365	trimer-stabilizing mutations that resist trimer conformational transition and exposure of CD4i epitopes	2338:2440	These limitations have been mitigated through recent structure-guided approaches and include trimer-stabilizing mutations that resist trimer conformational transition and exposure of CD4i epitopes.					
32817216	8	88	theme	HIV-1	2022:2026	arg1	trimers					2037:2043	HIV-1 envelope trimers	2022:2043	HIV-1 envelope trimers	2022:2043	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	6	89	theme	high-mannose	1512:1523	arg1	glycans					1525:1531	high-mannose glycans	1512:1531	high-mannose glycans	1512:1531	In rhesus macaques, BMS-529 complexed to CD4 bs-targeting ch.SOSIP immunogen induced stronger neutralization against tier 2 pseudoviruses bearing high-mannose glycans than noncomplexed ch.SOSIP trimer immunogen.					
32817216	11	90	theme	inhibitor	2707:2715	arg1	BMS-626529					2717:2726	the viral entry inhibitor BMS-626529	2691:2726	the viral entry inhibitor BMS-626529	2691:2726	In this study, we report that the viral entry inhibitor BMS-626529 restricts trimer conformational transition and improves the immunogenicity of select Env trimer immunogens.					
32817216	3	91	theme	non-bNAb	740:747	arg1	epitopes					749:756	CD4-induced (CD4i) non-bNAb epitopes	721:756	CD4-induced (CD4i) non-bNAb epitopes	721:756	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	8	92	theme	trimers	2037:2043	arg1	forms					2013:2017	noncomplexed trimers.IMPORTANCE Soluble forms	1973:2017	noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers	1973:2043	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	4	93	gly	glycosylation	1012:1024	arg1	epitopes					997:1004	CD4i epitopes	992:1004	CD4i epitopes	992:1004	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	4	94	from	trimers	797:803	arg1	micelles					818:825	detergent micelles	808:825	detergent micelles	808:825	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	1	95	theme	viral	126:130	arg1	inhibitors					138:147	Small-molecule viral entry inhibitors	111:147	Small-molecule viral entry inhibitors	111:147	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	1	95	theme	viral	126:130	arg1	BMS-626529					158:167	BMS-626529	158:167	BMS-626529	158:167	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	7	96	theme	rhesus	1626:1631	arg1	macaques					1633:1640	rhesus macaques	1626:1640	rhesus macaques	1626:1640	When immunized with gp150 complexed to BMS-529, rhesus macaques showed neutralization against tier 2 pseudoviruses with targeted glycan deletion and high-mannose glycan enrichment.					
32817216	5	97	theme	autologous	1236:1245	arg1	pseudoviruses					1299:1311	both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses	1231:1311	both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses	1231:1311	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	9	98	theme	trimer	2379:2384	arg1	transition					2401:2410	trimer conformational transition	2379:2410	trimer conformational transition	2379:2410	These limitations have been mitigated through recent structure-guided approaches and include trimer-stabilizing mutations that resist trimer conformational transition and exposure of CD4i epitopes.					
32817216	8	99	theme	conformational	2053:2066	arg1	heterogeneity					2068:2080	conformational heterogeneity	2053:2080	conformational heterogeneity	2053:2080	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	4	100	attach	isolated	839:846	arg1	cells					857:861	CHO cells	853:861	CHO cells	853:861	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	4	100	attach	isolated	839:846	arg2	micelles					818:825	detergent micelles	808:825	detergent micelles	808:825	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	11	101	theme	conformational	2745:2758	arg1	transition					2760:2769	trimer conformational transition	2738:2769	trimer conformational transition	2738:2769	In this study, we report that the viral entry inhibitor BMS-626529 restricts trimer conformational transition and improves the immunogenicity of select Env trimer immunogens.					
32817216	4	102	theme	CD4	919:921	arg1	bs					937:938	bs	937:938	bs	937:938	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	4	102	theme	CD4	919:921	arg1	site					931:934	CD4 binding site	919:934	the CD4 binding site (bs) CH103 bNAb lineage	915:958	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	5	103	theme	neutralization	1201:1214	arg1	titers					1216:1221	higher neutralization titers	1194:1221	higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses	1194:1311	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	0	104	theme	Viral	88:92	arg1	Inhibitor					100:108	a Small-Molecule Viral Entry Inhibitor	71:108	a Small-Molecule Viral Entry Inhibitor	71:108	Antigenicity and Immunogenicity of HIV-1 Envelope Trimers Complexed to a Small-Molecule Viral Entry Inhibitor.					
32817216	4	105	theme	site	931:934	arg1	lineage					952:958	the CD4 binding site (bs) CH103 bNAb lineage	915:958	the CD4 binding site (bs) CH103 bNAb lineage	915:958	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	9	106	theme	CD4i	2428:2431	arg1	epitopes					2433:2440	CD4i epitopes	2428:2440	CD4i epitopes	2428:2440	These limitations have been mitigated through recent structure-guided approaches and include trimer-stabilizing mutations that resist trimer conformational transition and exposure of CD4i epitopes.					
32817216	3	107	dep	CD4-induced	721:731	arg1	CD4i					734:737	CD4i	734:737	CD4i	734:737	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	8	108	theme	select	1876:1881	arg1	trimers					1898:1904	select chimeric SOSIP trimers	1876:1904	select chimeric SOSIP trimers	1876:1904	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	11	109	theme	viral	2695:2699	arg1	BMS-626529					2717:2726	the viral entry inhibitor BMS-626529	2691:2726	the viral entry inhibitor BMS-626529	2691:2726	In this study, we report that the viral entry inhibitor BMS-626529 restricts trimer conformational transition and improves the immunogenicity of select Env trimer immunogens.					
32817216	8	110	theme	SOSIP	1892:1896	arg1	trimers					1898:1904	select chimeric SOSIP trimers	1876:1904	select chimeric SOSIP trimers	1876:1904	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	2	111	theme	trimer	467:472	arg1	transitions					489:499	trimer conformational transitions	467:499	trimer conformational transitions	467:499	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	2	112	theme	soluble	349:355	arg1	SOSIP					372:376	clade C soluble chimeric gp140 SOSIP	341:376	clade C soluble chimeric gp140 SOSIP (ch.SOSIP)	341:387	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	2	112	theme	soluble	349:355	arg1	ch.SOSIP					379:386	ch.SOSIP	379:386	ch.SOSIP	379:386	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	8	113	theme	epitopes	2125:2132	arg1	CD4i					2107:2110	CD4i	2107:2110	CD4i	2107:2110	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	8	113	theme	epitopes	2125:2132	arg1	exposure					2113:2120	CD4-induced (CD4i) exposure	2094:2120	CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses	2094:2242	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	4	114	with	exposure	980:987	arg1	preponderance					1041:1053	a preponderance	1039:1053	a preponderance of high-mannose glycans	1039:1077	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	7	115	theme	high-mannose	1727:1738	arg1	enrichment					1747:1756	high-mannose glycan enrichment	1727:1756	high-mannose glycan enrichment	1727:1756	When immunized with gp150 complexed to BMS-529, rhesus macaques showed neutralization against tier 2 pseudoviruses with targeted glycan deletion and high-mannose glycan enrichment.					
32817216	8	116	theme	broad	2206:2210	arg1	responses					2234:2242	broad neutralizing antibody responses	2206:2242	broad neutralizing antibody responses	2206:2242	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	4	117	theme	lineage	952:958	arg1	intermediates					898:910	intermediates	898:910	intermediates	898:910	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	4	117	theme	lineage	952:958	arg1	UCA					890:892	UCA	890:892	UCA	890:892	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	2	118	theme	clade	341:345	arg1	SOSIP					372:376	clade C soluble chimeric gp140 SOSIP	341:376	clade C soluble chimeric gp140 SOSIP (ch.SOSIP)	341:387	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	2	118	theme	clade	341:345	arg1	ch.SOSIP					379:386	ch.SOSIP	379:386	ch.SOSIP	379:386	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	6	119	theme	bs-targeting	1411:1422	arg1	immunogen					1433:1441	CD4 bs-targeting ch.SOSIP immunogen	1407:1441	CD4 bs-targeting ch.SOSIP immunogen	1407:1441	In rhesus macaques, BMS-529 complexed to CD4 bs-targeting ch.SOSIP immunogen induced stronger neutralization against tier 2 pseudoviruses bearing high-mannose glycans than noncomplexed ch.SOSIP trimer immunogen.					
32817216	5	120	theme	glycan-targeting	1113:1128	arg1	immunogen					1139:1147	BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen	1092:1147	BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen	1092:1147	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	8	121	theme	tier	1919:1922	arg1	antibodies					1939:1948	tier 2 neutralizing antibodies	1919:1948	tier 2 neutralizing antibodies of higher potency	1919:1966	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	8	122	theme	antibody	2225:2232	arg1	responses					2234:2242	broad neutralizing antibody responses	2206:2242	broad neutralizing antibody responses	2206:2242	These results demonstrated that stabilization of Env trimer conformation with BMS-529 improved the immunogenicity of select chimeric SOSIP trimers and elicited tier 2 neutralizing antibodies of higher potency than noncomplexed trimers.IMPORTANCE Soluble forms of HIV-1 envelope trimers exhibit conformational heterogeneity and undergo CD4-induced (CD4i) exposure of epitopes of non-neutralizing antibodies that can potentially hinder induction of broad neutralizing antibody responses.					
32817216	5	123	theme	tier	1292:1295	arg1	pseudoviruses					1299:1311	both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses	1231:1311	both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses	1231:1311	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	0	124	theme	Envelope	41:48	arg1	Trimers					50:56	HIV-1 Envelope Trimers	35:56	HIV-1 Envelope Trimers	35:56	Antigenicity and Immunogenicity of HIV-1 Envelope Trimers Complexed to a Small-Molecule Viral Entry Inhibitor.					
32817216	1	125	theme	Env	283:285	arg1	trimers					287:293	Env trimers	283:293	Env trimers	283:293	Small-molecule viral entry inhibitors, such as BMS-626529 (BMS-529), allosterically block CD4 binding to HIV-1 envelope (Env) and inhibit CD4-induced structural changes in Env trimers.					
32817216	7	126	theme	targeted	1698:1705	arg1	deletion					1714:1721	targeted glycan deletion	1698:1721	targeted glycan deletion	1698:1721	When immunized with gp150 complexed to BMS-529, rhesus macaques showed neutralization against tier 2 pseudoviruses with targeted glycan deletion and high-mannose glycan enrichment.					
32817216	2	127	theme	transmembrane	428:440	arg1	gp150					450:454	gp150	450:454	gp150	450:454	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	2	127	theme	transmembrane	428:440	arg1	domain					442:447	intact transmembrane domain	421:447	intact transmembrane domain (gp150)	421:455	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	3	128	theme	neutralizing	614:625	arg1	bNAbs					639:643	bNAbs	639:643	bNAbs	639:643	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	3	128	theme	neutralizing	614:625	arg1	antibodies					627:636	broadly neutralizing antibodies	606:636	broadly neutralizing antibodies (bNAbs)	606:644	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	4	129	theme	BMS-529-complexed	773:789	arg1	trimers					797:803	BMS-529-complexed gp150 trimers	773:803	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells,	773:862	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	2	130	theme	gp140	366:370	arg1	SOSIP					372:376	clade C soluble chimeric gp140 SOSIP	341:376	clade C soluble chimeric gp140 SOSIP (ch.SOSIP)	341:387	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	2	130	theme	gp140	366:370	arg1	ch.SOSIP					379:386	ch.SOSIP	379:386	ch.SOSIP	379:386	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	4	131	theme	CH103	941:945	arg1	lineage					952:958	the CD4 binding site (bs) CH103 bNAb lineage	915:958	the CD4 binding site (bs) CH103 bNAb lineage	915:958	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	5	132	theme	BMS-529-complexed	1092:1108	arg1	immunogen					1139:1147	BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen	1092:1147	BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen	1092:1147	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	4	133	theme	epitopes	997:1004	arg1	pattern					1026:1032	a glycosylation pattern	1010:1032	a glycosylation pattern with a preponderance of high-mannose glycans	1010:1077	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	4	133	theme	epitopes	997:1004	arg1	exposure					980:987	limited exposure	972:987	limited exposure of CD4i epitopes	972:1004	BMS-529-complexed gp150 trimers in detergent micelles, which were isolated from CHO cells, bound to bNAbs, including UCA and intermediates of the CD4 binding site (bs) CH103 bNAb lineage, and showed limited exposure of CD4i epitopes and a glycosylation pattern with a preponderance of high-mannose glycans.					
32817216	5	134	theme	immunized	1176:1184	arg1	animals					1186:1192	immunized animals	1176:1192	immunized animals	1176:1192	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	3	135	theme	ancestor	676:683	arg1	antibodies					691:700	their unmutated common ancestor (UCA) antibodies	653:700	their unmutated common ancestor (UCA) antibodies	653:700	When complexed to BMS-529, ch.SOSIP trimers retained their binding to broadly neutralizing antibodies (bNAbs) and to their unmutated common ancestor (UCA) antibodies, while exposure of CD4-induced (CD4i) non-bNAb epitopes was inhibited.					
32817216	5	136	theme	noncomplexed	1343:1354	arg1	ch.SOSIP					1356:1363	the noncomplexed ch.SOSIP	1339:1363	the noncomplexed ch.SOSIP	1339:1363	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
32817216	2	137	theme	BMS-529	330:336	arg1	binding					319:325	the binding	315:325	the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150)	315:455	Here, we show that the binding of BMS-529 to clade C soluble chimeric gp140 SOSIP (ch.SOSIP) and membrane-bound trimers with intact transmembrane domain (gp150) prevented trimer conformational transitions and enhanced their immunogenicity.					
32817216	5	138	theme	higher	1194:1199	arg1	titers					1216:1221	higher neutralization titers	1194:1221	higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses	1194:1311	In rabbits, BMS-529-complexed V3 glycan-targeting ch.SOSIP immunogen induced in the majority of immunized animals higher neutralization titers against both autologous and select high mannose-bearing heterologous tier 2 pseudoviruses than those immunized with the noncomplexed ch.SOSIP.					
33625241	2	0	theme	cross-coupling	374:387	arg1	reactions					389:397	stereoinvertive sp3-sp2 cross-coupling reactions	350:397	stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system	350:522	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	0	1	theme	2-Deoxy-α-C-glycosides	85:106	arg1	Synthesis					72:80	Direct α-C-Glycosylation: Synthesis	46:80	Direct α-C-Glycosylation: Synthesis of 2-Deoxy-α-C-glycosides	46:106	β-Glycosyl Trifluoroborates as Precursors for Direct α-C-Glycosylation: Synthesis of 2-Deoxy-α-C-glycosides.					
33625241	2	2	with	derivatives	431:441	arg1	halide					462:467	aryl or vinyl halide	448:467	halide	462:467	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	2	3	theme	sp3-sp2	366:372	arg1	reactions					389:397	stereoinvertive sp3-sp2 cross-coupling reactions	350:397	stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system	350:522	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	3	4	link	C-linked	574:581	arg1	analogues					604:612	C-linked 2'-deoxydisaccharide analogues	574:612	C-linked 2'-deoxydisaccharide analogues	574:612	Hydrogenation of the vinyl C-glycosides afforded C-linked 2'-deoxydisaccharide analogues.					
33625241	3	5	theme	C-linked	574:581	arg1	analogues					604:612	C-linked 2'-deoxydisaccharide analogues	574:612	C-linked 2'-deoxydisaccharide analogues	574:612	Hydrogenation of the vinyl C-glycosides afforded C-linked 2'-deoxydisaccharide analogues.					
33625241	2	6	theme	derivatives	431:441	arg1	reactions					389:397	stereoinvertive sp3-sp2 cross-coupling reactions	350:397	stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system	350:522	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	2	7	theme	catalytic	507:515	arg1	system					517:522	a photoredox/nickel dual catalytic system	482:522	a photoredox/nickel dual catalytic system	482:522	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	3	8	theme	2'-deoxydisaccharide	583:602	arg1	analogues					604:612	C-linked 2'-deoxydisaccharide analogues	574:612	C-linked 2'-deoxydisaccharide analogues	574:612	Hydrogenation of the vinyl C-glycosides afforded C-linked 2'-deoxydisaccharide analogues.					
33625241	1	9	theme	useful	201:206	arg1	tools					208:212	useful tools	201:212	useful tools for investigation of the biological functions of glycans	201:269	C-Glycosides are metabolically stable mimics of natural O-glycosides and are expected to be useful tools for investigation of the biological functions of glycans.					
33625241	2	10	theme	boronic	418:424	arg1	derivatives					431:441	2-deoxyglycosyl boronic acid derivatives	402:441	2-deoxyglycosyl boronic acid derivatives	402:441	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	2	11	theme	aryl	319:322	arg1	C-glycosides					334:345	aryl and vinyl C-glycosides	319:345	C-glycosides	334:345	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	2	12	theme	2-deoxyglycosyl	402:416	arg1	derivatives					431:441	2-deoxyglycosyl boronic acid derivatives	402:441	2-deoxyglycosyl boronic acid derivatives	402:441	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	2	13	theme	vinyl	456:460	arg1	halide					462:467	aryl or vinyl halide	448:467	halide	462:467	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	2	14	theme	series	309:314	arg1	synthesis					294:302	the synthesis	290:302	the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system	290:522	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	0	15	theme	Direct	46:51	arg1	Synthesis					72:80	Direct α-C-Glycosylation: Synthesis	46:80	Direct α-C-Glycosylation: Synthesis of 2-Deoxy-α-C-glycosides	46:106	β-Glycosyl Trifluoroborates as Precursors for Direct α-C-Glycosylation: Synthesis of 2-Deoxy-α-C-glycosides.					
33625241	2	16	theme	aryl	448:451	arg1	halide					462:467	aryl or vinyl halide	448:467	halide	462:467	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	2	17	theme	dual	502:505	arg1	system					517:522	a photoredox/nickel dual catalytic system	482:522	a photoredox/nickel dual catalytic system	482:522	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	1	18	theme	biological	239:248	arg1	functions					250:258	the biological functions	235:258	the biological functions of glycans	235:269	C-Glycosides are metabolically stable mimics of natural O-glycosides and are expected to be useful tools for investigation of the biological functions of glycans.					
33625241	2	19	theme	photoredox/nickel	484:500	arg1	system					517:522	a photoredox/nickel dual catalytic system	482:522	a photoredox/nickel dual catalytic system	482:522	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	2	20	theme	stereoinvertive	350:364	arg1	reactions					389:397	stereoinvertive sp3-sp2 cross-coupling reactions	350:397	stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system	350:522	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	0	21	theme	α-C-Glycosylation	53:69	arg1	Synthesis					72:80	Direct α-C-Glycosylation: Synthesis	46:80	Direct α-C-Glycosylation: Synthesis of 2-Deoxy-α-C-glycosides	46:106	β-Glycosyl Trifluoroborates as Precursors for Direct α-C-Glycosylation: Synthesis of 2-Deoxy-α-C-glycosides.					
33625241	3	22	theme	vinyl	546:550	arg1	C-glycosides					552:563	the vinyl C-glycosides	542:563	the vinyl C-glycosides	542:563	Hydrogenation of the vinyl C-glycosides afforded C-linked 2'-deoxydisaccharide analogues.					
33625241	1	23	theme	natural	157:163	arg1	O-glycosides					165:176	natural O-glycosides	157:176	natural O-glycosides	157:176	C-Glycosides are metabolically stable mimics of natural O-glycosides and are expected to be useful tools for investigation of the biological functions of glycans.					
33625241	1	24	theme	functions	250:258	arg1	investigation					218:230	investigation	218:230	investigation of the biological functions of glycans	218:269	C-Glycosides are metabolically stable mimics of natural O-glycosides and are expected to be useful tools for investigation of the biological functions of glycans.					
33625241	2	25	theme	vinyl	328:332	arg1	C-glycosides					334:345	aryl and vinyl C-glycosides	319:345	C-glycosides	334:345	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	2	26	theme	C-glycosides	334:345	arg1	series					309:314	a series	307:314	a series of aryl and vinyl C-glycosides	307:345	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	3	27	theme	C-glycosides	552:563	arg1	Hydrogenation					525:537	Hydrogenation	525:537	Hydrogenation of the vinyl C-glycosides	525:563	Hydrogenation of the vinyl C-glycosides afforded C-linked 2'-deoxydisaccharide analogues.					
33625241	2	28	theme	acid	426:429	arg1	derivatives					431:441	2-deoxyglycosyl boronic acid derivatives	402:441	2-deoxyglycosyl boronic acid derivatives	402:441	Here, we describe the synthesis of a series of aryl and vinyl C-glycosides by stereoinvertive sp3-sp2 cross-coupling reactions of 2-deoxyglycosyl boronic acid derivatives with aryl or vinyl halide, mediated by a photoredox/nickel dual catalytic system.					
33625241	1	29	theme	glycans	263:269	arg1	functions					250:258	the biological functions	235:258	the biological functions of glycans	235:269	C-Glycosides are metabolically stable mimics of natural O-glycosides and are expected to be useful tools for investigation of the biological functions of glycans.					
33625241	1	30	dep	stable	140:145	arg1	mimics					147:152	mimics	147:152	mimics of natural O-glycosides	147:176	C-Glycosides are metabolically stable mimics of natural O-glycosides and are expected to be useful tools for investigation of the biological functions of glycans.					
32975931	7	0	theme	energies	1644:1651	arg1	delay					1584:1588	delay	1584:1588	delay between the two laser pulses, and that of their pulse energies	1584:1651	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	6	1	theme	sensitivity	994:1004	arg1	increase					1006:1013	a sensitivity increase	992:1013	a sensitivity increase	992:1013	Compared to the current gold standard, the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10 is achieved.					
32975931	6	1	theme	sensitivity	994:1004	arg1	[M					973:974	[M + Na]+ adducts	973:989	[M	973:974	Compared to the current gold standard, the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10 is achieved.					
32975931	2	2	theme	high-throughput	329:343	arg1	profiling					354:362	high-throughput N-glycan profiling	329:362	high-throughput N-glycan profiling	329:362	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	1	3	theme	infections	219:228	arg1	types					164:168	different types	154:168	different types of cancer, (auto)immune diseases, and also viral infections	154:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	7	4	theme	tandem	1339:1344	arg1	MS.					1346:1348	low-energy collision-induced dissociation tandem MS.	1297:1348	low-energy collision-induced dissociation tandem MS. In another set of experiments	1297:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	2	5	theme	mass	275:278	arg1	tool					320:323	an important tool	307:323	an important tool for high-throughput N-glycan profiling	307:362	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	2	5	theme	mass	275:278	arg1	MALDI-MS					294:301	MALDI-MS	294:301	MALDI-MS	294:301	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	2	5	theme	mass	275:278	arg1	spectrometry					280:291	Matrix-assisted laser desorption/ionization mass spectrometry	231:291	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	231:302	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	7	6	theme	collision-induced	1308:1324	arg1	MS.					1346:1348	low-energy collision-induced dissociation tandem MS.	1297:1348	low-energy collision-induced dissociation tandem MS. In another set of experiments	1297:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	2	7	theme	laser	247:251	arg1	tool					320:323	an important tool	307:323	an important tool for high-throughput N-glycan profiling	307:362	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	2	7	theme	laser	247:251	arg1	MALDI-MS					294:301	MALDI-MS	294:301	MALDI-MS	294:301	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	2	7	theme	laser	247:251	arg1	spectrometry					280:291	Matrix-assisted laser desorption/ionization mass spectrometry	231:291	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	231:302	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	7	8	from	information	1152:1162	arg1	composition					1171:1181	the composition	1167:1181	the composition of complex N-glycans	1167:1202	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	8	9	theme	ion	1724:1726	arg1	abundances					1728:1737	molecular ion abundances	1714:1737	molecular ion abundances	1714:1737	In this way, analytical conditions were identified at which molecular ion abundances were maximized and fragmentation reactions minimized.					
32975931	7	10	from	set	1361:1363	arg1	use					1290:1292	use	1290:1292	use of low-energy collision-induced dissociation tandem MS. In another set of experiments	1290:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	8	11	theme	analytical	1667:1676	arg1	conditions					1678:1687	analytical conditions	1667:1687	analytical conditions	1667:1687	In this way, analytical conditions were identified at which molecular ion abundances were maximized and fragmentation reactions minimized.					
32975931	7	12	theme	laser	1606:1610	arg1	pulses					1612:1617	the two laser pulses	1598:1617	the two laser pulses	1598:1617	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	3	13	theme	N-glycan	520:527	arg1	profiling					529:537	spatially correlated N-glycan profiling	499:537	spatially correlated N-glycan profiling from tissue sections	499:558	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
32975931	1	14	theme	auto	182:185	arg1	diseases					194:201	(auto)immune diseases	181:201	(auto)immune diseases	181:201	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	7	15	theme	input	1502:1506	arg1	parameters					1508:1517	relevant input parameters	1493:1517	relevant input parameters	1493:1517	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	16	theme	cerebellum	1270:1279	arg1	sections					1252:1259	thin tissue sections	1240:1259	thin tissue sections of human cerebellum	1240:1279	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	5	17	theme	molecular	818:826	arg1	[M					828:829	molecular [M	818:829	molecular [M	818:829	We demonstrate that with MALDI-2 the sensitivity for the detection of molecular [M - H]- species of N-glycans increased by about 3 orders of magnitude.					
32975931	7	18	from	pressure	1534:1541	arg1	source					1576:1581	the ion source	1568:1581	the ion source	1568:1581	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	4	19	theme	mass	688:691	arg1	Daltonics					736:744	Daltonics	736:744	Daltonics	736:744	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	4	19	theme	mass	688:691	arg1	spectrometer					693:704	a trapped ion mobility quadrupole time-of-flight mass spectrometer	639:704	a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics)	639:745	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	7	20	theme	tissue	1245:1250	arg1	sections					1252:1259	thin tissue sections	1240:1259	thin tissue sections of human cerebellum	1240:1279	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	9	21	theme	ion	1813:1815	arg1	MALDI-2-MSI					1822:1832	negative ion mode MALDI-2-MSI	1804:1832	negative ion mode MALDI-2-MSI	1804:1832	The use of negative ion mode MALDI-2-MSI could constitute a valuable tool in glycobiology research.					
32975931	6	22	theme	mode	956:959	arg1	analysis					961:968	the positive ion mode analysis	939:968	the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10	939:1037	Compared to the current gold standard, the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10 is achieved.					
32975931	7	23	theme	complex	1186:1192	arg1	N-glycans					1194:1202	complex N-glycans	1186:1202	complex N-glycans	1186:1202	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	4	24	theme	ion	649:651	arg1	Daltonics					736:744	Daltonics	736:744	Daltonics	736:744	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	4	24	theme	ion	649:651	arg1	spectrometer					693:704	a trapped ion mobility quadrupole time-of-flight mass spectrometer	639:704	a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics)	639:745	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	5	25	theme	species	837:843	arg1	detection					805:813	the detection	801:813	the detection of molecular [M - H]- species of N-glycans	801:856	We demonstrate that with MALDI-2 the sensitivity for the detection of molecular [M - H]- species of N-glycans increased by about 3 orders of magnitude.					
32975931	7	26	theme	ion	1572:1574	arg1	source					1576:1581	the ion source	1568:1581	the ion source	1568:1581	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	6	27	theme	positive	943:950	arg1	mode					956:959	the positive ion mode	939:959	the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10	939:1037	Compared to the current gold standard, the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10 is achieved.					
32975931	4	28	theme	quadrupole	662:671	arg1	Daltonics					736:744	Daltonics	736:744	Daltonics	736:744	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	4	28	theme	quadrupole	662:671	arg1	spectrometer					693:704	a trapped ion mobility quadrupole time-of-flight mass spectrometer	639:704	a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics)	639:745	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	0	29	theme	Spectrometry	62:73	arg1	Imaging					75:81	Mass Spectrometry Imaging	57:81	Mass Spectrometry Imaging	57:81	MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging.					
32975931	1	30	theme	different	154:162	arg1	types					164:168	different types	154:168	different types of cancer, (auto)immune diseases, and also viral infections	154:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	9	31	theme	valuable	1853:1860	arg1	tool					1862:1865	a valuable tool	1851:1865	a valuable tool in glycobiology research	1851:1890	The use of negative ion mode MALDI-2-MSI could constitute a valuable tool in glycobiology research.					
32975931	7	32	theme	[M	1109:1110	arg1	behavior					1097:1104	the advantageous fragmentation behavior	1066:1104	the advantageous fragmentation behavior of [M	1066:1110	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	33	theme	cooling	1553:1559	arg1	gas					1561:1563	the N2 cooling gas	1546:1563	the N2 cooling gas	1546:1563	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	34	theme	mass	1432:1435	arg1	spectrometer					1437:1448	a modified Synapt G2-S QTOF mass spectrometer	1404:1448	a modified Synapt G2-S QTOF mass spectrometer (Waters)	1404:1457	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	34	theme	mass	1432:1435	arg1	Waters					1451:1456	Waters	1451:1456	Waters	1451:1456	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	6	35	theme	adducts	983:989	arg1	analysis					961:968	the positive ion mode analysis	939:968	the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10	939:1037	Compared to the current gold standard, the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10 is achieved.					
32975931	7	36	theme	advantageous	1070:1081	arg1	behavior					1097:1104	the advantageous fragmentation behavior	1066:1104	the advantageous fragmentation behavior of [M	1066:1110	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	1	37	from	players	108:114	arg1	pathologies					132:142	pathologies	132:142	pathologies including different types of cancer, (auto)immune diseases, and also viral infections	132:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	1	37	from	players	108:114	arg1	types					164:168	different types	154:168	different types of cancer, (auto)immune diseases, and also viral infections	154:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	1	37	from	players	108:114	arg1	variety					121:127	a variety	119:127	a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections	119:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	3	38	from	sections	551:558	arg1	profiling					529:537	spatially correlated N-glycan profiling	499:537	spatially correlated N-glycan profiling from tissue sections	499:558	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
32975931	3	39	theme	MALDI-MS	431:438	arg1	MSI					449:451	MSI	449:451	MSI	449:451	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
32975931	3	39	theme	MALDI-MS	431:438	arg1	imaging					440:446	MALDI-MS imaging	431:446	MALDI-MS imaging (MSI)	431:452	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
32975931	9	40	theme	MALDI-2-MSI	1822:1832	arg1	use					1797:1799	The use	1793:1799	The use of negative ion mode MALDI-2-MSI	1793:1832	The use of negative ion mode MALDI-2-MSI could constitute a valuable tool in glycobiology research.					
32975931	7	41	theme	Synapt	1415:1420	arg1	spectrometer					1437:1448	a modified Synapt G2-S QTOF mass spectrometer	1404:1448	a modified Synapt G2-S QTOF mass spectrometer (Waters)	1404:1457	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	41	theme	Synapt	1415:1420	arg1	Waters					1451:1456	Waters	1451:1456	Waters	1451:1456	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	1	42	theme	important	98:106	arg1	N-glycans					84:92	N-glycans	84:92	N-glycans	84:92	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	1	42	theme	important	98:106	arg1	players					108:114	important players	98:114	important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections	98:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	7	43	theme	N-glycans	1194:1202	arg1	composition					1171:1181	the composition	1167:1181	the composition of complex N-glycans	1167:1202	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	2	44	theme	MS	388:389	arg1	use					374:376	use	374:376	use of tandem MS	374:389	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	0	45	theme	N-Linked	37:44	arg1	Glycans					46:52	N-Linked Glycans	37:52	N-Linked Glycans	37:52	MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging.					
32975931	2	46	theme	important	310:318	arg1	tool					320:323	an important tool	307:323	an important tool for high-throughput N-glycan profiling	307:362	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	2	46	theme	important	310:318	arg1	spectrometry					280:291	Matrix-assisted laser desorption/ionization mass spectrometry	231:291	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	231:302	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	1	47	theme	diseases	194:201	arg1	types					164:168	different types	154:168	different types of cancer, (auto)immune diseases, and also viral infections	154:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	1	48	theme	pathologies	132:142	arg1	pathologies					132:142	pathologies	132:142	pathologies including different types of cancer, (auto)immune diseases, and also viral infections	132:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	1	48	theme	pathologies	132:142	arg1	types					164:168	different types	154:168	different types of cancer, (auto)immune diseases, and also viral infections	154:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	1	48	theme	pathologies	132:142	arg1	variety					121:127	a variety	119:127	a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections	119:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	3	49	theme	PNGase	474:479	arg1	treatment					483:491	PNGase F treatment	474:491	PNGase F treatment	474:491	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
32975931	0	50	dep	MALDI-2	0:6	arg1	Analysis					25:32	the Enhanced Analysis	12:32	the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging	12:81	MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging.					
32975931	7	51	theme	pulse	1638:1642	arg1	energies					1644:1651	their pulse energies	1632:1651	their pulse energies	1632:1651	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	52	theme	rich	1136:1139	arg1	information					1152:1162	rich structural information	1136:1162	rich structural information on the composition of complex N-glycans	1136:1202	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	3	53	with	combination	457:467	arg1	treatment					483:491	PNGase F treatment	474:491	PNGase F treatment	474:491	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
32975931	2	54	theme	N-glycan	345:352	arg1	profiling					354:362	high-throughput N-glycan profiling	329:362	high-throughput N-glycan profiling	329:362	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	1	55	theme	viral	213:217	arg1	infections					219:228	viral infections	213:228	viral infections	213:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	7	56	theme	experiments	1368:1378	arg1	set					1361:1363	another set	1353:1363	another set of experiments	1353:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	56	theme	experiments	1368:1378	arg1	experiments					1368:1378	experiments	1368:1378	experiments	1368:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	57	theme	MS.	1346:1348	arg1	use					1290:1292	use	1290:1292	use of low-energy collision-induced dissociation tandem MS. In another set of experiments	1290:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	8	58	theme	molecular	1714:1722	arg1	abundances					1728:1737	molecular ion abundances	1714:1737	molecular ion abundances	1714:1737	In this way, analytical conditions were identified at which molecular ion abundances were maximized and fragmentation reactions minimized.					
32975931	2	59	theme	desorption/ionization	253:273	arg1	tool					320:323	an important tool	307:323	an important tool for high-throughput N-glycan profiling	307:362	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	2	59	theme	desorption/ionization	253:273	arg1	MALDI-MS					294:301	MALDI-MS	294:301	MALDI-MS	294:301	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	2	59	theme	desorption/ionization	253:273	arg1	spectrometry					280:291	Matrix-assisted laser desorption/ionization mass spectrometry	231:291	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	231:302	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	1	60	theme	cancer	173:178	arg1	types					164:168	different types	154:168	different types of cancer, (auto)immune diseases, and also viral infections	154:228	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	5	61	theme	[M	828:829	arg1	detection					805:813	the detection	801:813	the detection of molecular [M - H]- species of N-glycans	801:856	We demonstrate that with MALDI-2 the sensitivity for the detection of molecular [M - H]- species of N-glycans increased by about 3 orders of magnitude.					
32975931	7	62	theme	dissociation	1326:1337	arg1	MS.					1346:1348	low-energy collision-induced dissociation tandem MS.	1297:1348	low-energy collision-induced dissociation tandem MS. In another set of experiments	1297:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	5	63	theme	H	833:833	arg1	species					837:843	H]- species	833:843	H]- species	833:843	We demonstrate that with MALDI-2 the sensitivity for the detection of molecular [M - H]- species of N-glycans increased by about 3 orders of magnitude.					
32975931	7	64	theme	low-energy	1297:1306	arg1	MS.					1346:1348	low-energy collision-induced dissociation tandem MS.	1297:1348	low-energy collision-induced dissociation tandem MS. In another set of experiments	1297:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	3	65	theme	correlated	509:518	arg1	profiling					529:537	spatially correlated N-glycan profiling	499:537	spatially correlated N-glycan profiling from tissue sections	499:558	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
32975931	7	66	theme	relevant	1493:1500	arg1	parameters					1508:1517	relevant input parameters	1493:1517	relevant input parameters	1493:1517	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	5	67	theme	N-glycans	848:856	arg1	[M					828:829	molecular [M	818:829	molecular [M	818:829	We demonstrate that with MALDI-2 the sensitivity for the detection of molecular [M - H]- species of N-glycans increased by about 3 orders of magnitude.					
32975931	4	68	theme	laser-induced	594:606	arg1	postionization					608:621	laser-induced postionization	594:621	laser-induced postionization	594:621	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	8	69	theme	fragmentation	1758:1770	arg1	reactions					1772:1780	fragmentation reactions	1758:1780	fragmentation reactions	1758:1780	In this way, analytical conditions were identified at which molecular ion abundances were maximized and fragmentation reactions minimized.					
32975931	3	70	theme	tissue	544:549	arg1	sections					551:558	tissue sections	544:558	tissue sections	544:558	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
32975931	7	71	theme	human	1264:1268	arg1	cerebellum					1270:1279	human cerebellum	1264:1279	human cerebellum	1264:1279	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	9	72	theme	negative	1804:1811	arg1	MALDI-2-MSI					1822:1832	negative ion mode MALDI-2-MSI	1804:1832	negative ion mode MALDI-2-MSI	1804:1832	The use of negative ion mode MALDI-2-MSI could constitute a valuable tool in glycobiology research.					
32975931	2	73	theme	Matrix-assisted	231:245	arg1	tool					320:323	an important tool	307:323	an important tool for high-throughput N-glycan profiling	307:362	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	2	73	theme	Matrix-assisted	231:245	arg1	MALDI-MS					294:301	MALDI-MS	294:301	MALDI-MS	294:301	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	2	73	theme	Matrix-assisted	231:245	arg1	spectrometry					280:291	Matrix-assisted laser desorption/ionization mass spectrometry	231:291	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	231:302	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	7	74	from	use	1290:1292	arg1	set					1361:1363	another set	1353:1363	another set of experiments	1353:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	74	from	use	1290:1292	arg1	experiments					1368:1378	experiments	1368:1378	experiments	1368:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	9	75	theme	mode	1817:1820	arg1	MALDI-2-MSI					1822:1832	negative ion mode MALDI-2-MSI	1804:1832	negative ion mode MALDI-2-MSI	1804:1832	The use of negative ion mode MALDI-2-MSI could constitute a valuable tool in glycobiology research.					
32975931	0	76	theme	Mass	57:60	arg1	Spectrometry					62:73	Mass Spectrometry	57:73	Mass Spectrometry Imaging	57:81	MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging.					
32975931	7	77	from	MS.	1346:1348	arg1	set					1361:1363	another set	1353:1363	another set of experiments	1353:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	77	from	MS.	1346:1348	arg1	experiments					1368:1378	experiments	1368:1378	experiments	1368:1378	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	6	78	theme	ion	952:954	arg1	mode					956:959	the positive ion mode	939:959	the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10	939:1037	Compared to the current gold standard, the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10 is achieved.					
32975931	7	79	theme	thin	1240:1243	arg1	sections					1252:1259	thin tissue sections	1240:1259	thin tissue sections of human cerebellum	1240:1279	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	4	80	theme	trapped	641:647	arg1	Daltonics					736:744	Daltonics	736:744	Daltonics	736:744	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	4	80	theme	trapped	641:647	arg1	spectrometer					693:704	a trapped ion mobility quadrupole time-of-flight mass spectrometer	639:704	a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics)	639:745	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	4	81	dep	Daltonics	736:744	arg1	Bruker					729:734	Bruker	729:734	Bruker	729:734	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	7	82	theme	spectrometer	1437:1448	arg1	use					1397:1399	use	1397:1399	use of a modified Synapt G2-S QTOF mass spectrometer (Waters)	1397:1457	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	4	83	theme	mobility	653:660	arg1	Daltonics					736:744	Daltonics	736:744	Daltonics	736:744	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	4	83	theme	mobility	653:660	arg1	spectrometer					693:704	a trapped ion mobility quadrupole time-of-flight mass spectrometer	639:704	a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics)	639:745	Here we coupled laser-induced postionization, or MALDI-2, to a trapped ion mobility quadrupole time-of-flight mass spectrometer (timsTOF fleX MALDI-2, Bruker Daltonics).					
32975931	6	84	theme	10	1036:1037	arg1	factor					1026:1031	about a factor	1018:1031	about a factor of 10	1018:1037	Compared to the current gold standard, the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10 is achieved.					
32975931	7	85	theme	QTOF	1427:1430	arg1	spectrometer					1437:1448	a modified Synapt G2-S QTOF mass spectrometer	1404:1448	a modified Synapt G2-S QTOF mass spectrometer (Waters)	1404:1457	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	85	theme	QTOF	1427:1430	arg1	Waters					1451:1456	Waters	1451:1456	Waters	1451:1456	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	8	86	dep	identified	1694:1703	arg1	minimized					1782:1790	minimized	1782:1790	minimized	1782:1790	In this way, analytical conditions were identified at which molecular ion abundances were maximized and fragmentation reactions minimized.					
32975931	8	86	dep	identified	1694:1703	arg1	maximized					1744:1752	maximized	1744:1752	were maximized	1739:1752	In this way, analytical conditions were identified at which molecular ion abundances were maximized and fragmentation reactions minimized.					
32975931	7	87	theme	fragmentation	1083:1095	arg1	behavior					1097:1104	the advantageous fragmentation behavior	1066:1104	the advantageous fragmentation behavior of [M	1066:1110	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	88	theme	gas	1561:1563	arg1	pressure					1534:1541	particular pressure	1523:1541	particular pressure of the N2 cooling gas in the ion source	1523:1581	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	9	89	theme	glycobiology	1870:1881	arg1	research					1883:1890	glycobiology research	1870:1890	glycobiology research	1870:1890	The use of negative ion mode MALDI-2-MSI could constitute a valuable tool in glycobiology research.					
32975931	7	90	dep	ions	1118:1121	arg1	obtained					1217:1224	obtained	1217:1224	obtained	1217:1224	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	91	theme	N2	1550:1551	arg1	gas					1561:1563	the N2 cooling gas	1546:1563	the N2 cooling gas	1546:1563	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	0	92	theme	Enhanced	16:23	arg1	Analysis					25:32	the Enhanced Analysis	12:32	the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging	12:81	MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging.					
32975931	5	93	theme	magnitude	889:897	arg1	orders					879:884	about 3 orders	871:884	about 3 orders of magnitude	871:897	We demonstrate that with MALDI-2 the sensitivity for the detection of molecular [M - H]- species of N-glycans increased by about 3 orders of magnitude.					
32975931	6	94	theme	Na	978:979	arg1	analysis					961:968	the positive ion mode analysis	939:968	the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10	939:1037	Compared to the current gold standard, the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10 is achieved.					
32975931	2	95	theme	structure	396:404	arg1	determination					406:418	structure determination	396:418	structure determination	396:418	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	3	96	theme	imaging	440:446	arg1	use					424:426	use	424:426	use of MALDI-MS imaging (MSI) in combination with PNGase F treatment	424:491	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
32975931	7	97	theme	G2-S	1422:1425	arg1	spectrometer					1437:1448	a modified Synapt G2-S QTOF mass spectrometer	1404:1448	a modified Synapt G2-S QTOF mass spectrometer (Waters)	1404:1457	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	97	theme	G2-S	1422:1425	arg1	Waters					1451:1456	Waters	1451:1456	Waters	1451:1456	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	98	theme	particular	1523:1532	arg1	pressure					1534:1541	particular pressure	1523:1541	particular pressure of the N2 cooling gas in the ion source	1523:1581	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	6	99	theme	[M	973:974	arg1	analysis					961:968	the positive ion mode analysis	939:968	the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10	939:1037	Compared to the current gold standard, the positive ion mode analysis of [M + Na]+ adducts, a sensitivity increase by about a factor of 10 is achieved.					
32975931	3	100	from	use	424:426	arg1	combination					457:467	combination	457:467	combination with PNGase F treatment	457:491	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
32975931	2	101	theme	tandem	381:386	arg1	MS					388:389	tandem MS	381:389	tandem MS	381:389	Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an important tool for high-throughput N-glycan profiling and, upon use of tandem MS, for structure determination.					
32975931	0	102	theme	Glycans	46:52	arg1	Analysis					25:32	the Enhanced Analysis	12:32	the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging	12:81	MALDI-2 for the Enhanced Analysis of N-Linked Glycans by Mass Spectrometry Imaging.					
32975931	7	103	theme	modified	1406:1413	arg1	spectrometer					1437:1448	a modified Synapt G2-S QTOF mass spectrometer	1404:1448	a modified Synapt G2-S QTOF mass spectrometer (Waters)	1404:1457	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	103	theme	modified	1406:1413	arg1	Waters					1451:1456	Waters	1451:1456	Waters	1451:1456	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	104	dep	behavior	1097:1104	arg1	ions					1118:1121	ions	1118:1121	ions	1118:1121	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	7	105	theme	structural	1141:1150	arg1	information					1152:1162	rich structural information	1136:1162	rich structural information on the composition of complex N-glycans	1136:1202	By exploiting the advantageous fragmentation behavior of [M - H]- ions, exceedingly rich structural information on the composition of complex N-glycans was moreover obtained directly from thin tissue sections of human cerebellum and upon use of low-energy collision-induced dissociation tandem MS. In another set of experiments, in this case by use of a modified Synapt G2-S QTOF mass spectrometer (Waters), we investigated the influence of relevant input parameters, in particular pressure of the N2 cooling gas in the ion source, delay between the two laser pulses, and that of their pulse energies.					
32975931	1	106	theme	immune	187:192	arg1	diseases					194:201	(auto)immune diseases	181:201	(auto)immune diseases	181:201	N-glycans are important players in a variety of pathologies including different types of cancer, (auto)immune diseases, and also viral infections.					
32975931	9	107	from	tool	1862:1865	arg1	research					1883:1890	glycobiology research	1870:1890	glycobiology research	1870:1890	The use of negative ion mode MALDI-2-MSI could constitute a valuable tool in glycobiology research.					
32975931	3	108	theme	F	481:481	arg1	treatment					483:491	PNGase F treatment	474:491	PNGase F treatment	474:491	By use of MALDI-MS imaging (MSI) in combination with PNGase F treatment, also spatially correlated N-glycan profiling from tissue sections becomes possible.					
34389709	3	0	theme	wide	550:553	arg1	scope					555:559	a wide scope	548:559	a wide scope of the coupling partners, including complex oligosaccharide and peptide substrates	548:642	It accommodates a wide scope of the coupling partners, including complex oligosaccharide and peptide substrates.					
34389709	4	1	theme	chains	835:840	arg1	elongation					794:803	elongation	794:803	elongation of the peptide and saccharide chains	794:840	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	0	2	theme	reductive	70:78	arg1	hydroglycosylation					80:97	Ni-catalyzed reductive hydroglycosylation	57:97	Ni-catalyzed reductive hydroglycosylation of alkynes	57:108	Facile access to C-glycosyl amino acids and peptides via Ni-catalyzed reductive hydroglycosylation of alkynes.					
34389709	2	3	with	reaction	442:449	arg1	bromides					522:529	common glycosyl bromides	506:529	common glycosyl bromides	506:529	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	1	4	theme	native	179:184	arg1	glycopeptides/proteins					186:207	the native glycopeptides/proteins	175:207	the native glycopeptides/proteins bearing O/N-glycosidic linkages	175:239	C-Glycosyl peptides/proteins are metabolically stable mimics of the native glycopeptides/proteins bearing O/N-glycosidic linkages, and are thus of great therapeutical potential.					
34389709	0	5	theme	alkynes	102:108	arg1	hydroglycosylation					80:97	Ni-catalyzed reductive hydroglycosylation	57:97	Ni-catalyzed reductive hydroglycosylation of alkynes	57:108	Facile access to C-glycosyl amino acids and peptides via Ni-catalyzed reductive hydroglycosylation of alkynes.					
34389709	2	6	theme	peptides	374:381	arg1	syntheses					328:336	the syntheses	324:336	the syntheses of vinyl C-glycosyl amino acids and peptides	324:381	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	4	7	theme	saccharide	824:833	arg1	chains					835:840	the peptide and saccharide chains	808:840	chains	835:840	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	8	theme	resultant	649:657	arg1	amenable					747:754	amenable	747:754	amenable	747:754	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	8	theme	resultant	649:657	arg1	acids					682:686	The resultant vinyl C-glycosyl amino acids	645:686	The resultant vinyl C-glycosyl amino acids	645:686	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	1	9	dep	stable	158:163	arg1	mimics					165:170	mimics	165:170	mimics of the native glycopeptides/proteins bearing O/N-glycosidic linkages	165:239	C-Glycosyl peptides/proteins are metabolically stable mimics of the native glycopeptides/proteins bearing O/N-glycosidic linkages, and are thus of great therapeutical potential.					
34389709	3	10	theme	coupling	568:575	arg1	oligosaccharide					605:619	complex oligosaccharide	597:619	complex oligosaccharide	597:619	It accommodates a wide scope of the coupling partners, including complex oligosaccharide and peptide substrates.					
34389709	3	10	theme	coupling	568:575	arg1	substrates					633:642	peptide substrates	625:642	peptide substrates	625:642	It accommodates a wide scope of the coupling partners, including complex oligosaccharide and peptide substrates.					
34389709	3	10	theme	coupling	568:575	arg1	partners					577:584	the coupling partners	564:584	the coupling partners	564:584	It accommodates a wide scope of the coupling partners, including complex oligosaccharide and peptide substrates.					
34389709	2	11	theme	reductive	413:421	arg1	reaction					442:449	a nickel-catalyzed reductive hydroglycosylation reaction	394:449	a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides	394:529	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	4	12	theme	common	713:718	arg1	groups					735:740	common O/N-protecting groups	713:740	common O/N-protecting groups	713:740	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	0	13	gly	hydroglycosylation	80:97	arg1	alkynes					102:108	alkynes	102:108	alkynes	102:108	Facile access to C-glycosyl amino acids and peptides via Ni-catalyzed reductive hydroglycosylation of alkynes.					
34389709	2	14	theme	hydroglycosylation	423:440	arg1	reaction					442:449	a nickel-catalyzed reductive hydroglycosylation reaction	394:449	a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides	394:529	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	2	15	theme	peptides	492:499	arg1	derivatives					461:471	alkyne derivatives	454:471	alkyne derivatives of amino acids and peptides	454:499	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	2	16	theme	vinyl	341:345	arg1	acids					364:368	vinyl C-glycosyl amino acids	341:368	vinyl C-glycosyl amino acids	341:368	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	1	17	theme	O/N-glycosidic	217:230	arg1	linkages					232:239	O/N-glycosidic linkages	217:239	O/N-glycosidic linkages	217:239	C-Glycosyl peptides/proteins are metabolically stable mimics of the native glycopeptides/proteins bearing O/N-glycosidic linkages, and are thus of great therapeutical potential.					
34389709	0	18	theme	Facile	0:5	arg1	access					7:12	Facile access	0:12	Facile access to C-glycosyl amino acids and peptides via Ni-catalyzed reductive hydroglycosylation of alkynes.	0:109	Facile access to C-glycosyl amino acids and peptides via Ni-catalyzed reductive hydroglycosylation of alkynes.					
34389709	3	19	theme	peptide	625:631	arg1	substrates					633:642	peptide substrates	625:642	peptide substrates	625:642	It accommodates a wide scope of the coupling partners, including complex oligosaccharide and peptide substrates.					
34389709	2	20	theme	nickel-catalyzed	396:411	arg1	reaction					442:449	a nickel-catalyzed reductive hydroglycosylation reaction	394:449	a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides	394:529	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	0	21	theme	C-glycosyl	17:26	arg1	acids					34:38	C-glycosyl amino acids	17:38	C-glycosyl amino acids	17:38	Facile access to C-glycosyl amino acids and peptides via Ni-catalyzed reductive hydroglycosylation of alkynes.					
34389709	3	22	theme	complex	597:603	arg1	oligosaccharide					605:619	complex oligosaccharide	597:619	complex oligosaccharide	597:619	It accommodates a wide scope of the coupling partners, including complex oligosaccharide and peptide substrates.					
34389709	2	23	theme	amino	476:480	arg1	acids					482:486	amino acids	476:486	amino acids	476:486	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	1	24	theme	C-Glycosyl	111:120	arg1	peptides/proteins					122:138	C-Glycosyl peptides/proteins	111:138	C-Glycosyl peptides/proteins	111:138	C-Glycosyl peptides/proteins are metabolically stable mimics of the native glycopeptides/proteins bearing O/N-glycosidic linkages, and are thus of great therapeutical potential.					
34389709	2	25	theme	derivatives	461:471	arg1	reaction					442:449	a nickel-catalyzed reductive hydroglycosylation reaction	394:449	a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides	394:529	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	4	26	theme	further	759:765	arg1	transformations					767:781	further transformations	759:781	further transformations	759:781	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	26	theme	further	759:765	arg1	elongation					794:803	elongation	794:803	elongation of the peptide and saccharide chains	794:840	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	27	theme	O/N-protecting	720:733	arg1	groups					735:740	common O/N-protecting groups	713:740	common O/N-protecting groups	713:740	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	2	28	theme	alkyne	454:459	arg1	derivatives					461:471	alkyne derivatives	454:471	alkyne derivatives of amino acids and peptides	454:499	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	2	29	theme	glycosyl	513:520	arg1	bromides					522:529	common glycosyl bromides	506:529	common glycosyl bromides	506:529	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	0	30	theme	amino	28:32	arg1	acids					34:38	C-glycosyl amino acids	17:38	C-glycosyl amino acids	17:38	Facile access to C-glycosyl amino acids and peptides via Ni-catalyzed reductive hydroglycosylation of alkynes.					
34389709	2	31	theme	acids	482:486	arg1	derivatives					461:471	alkyne derivatives	454:471	alkyne derivatives of amino acids and peptides	454:499	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	2	32	theme	common	506:511	arg1	bromides					522:529	common glycosyl bromides	506:529	common glycosyl bromides	506:529	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	4	33	theme	vinyl	659:663	arg1	amenable					747:754	amenable	747:754	amenable	747:754	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	33	theme	vinyl	659:663	arg1	acids					682:686	The resultant vinyl C-glycosyl amino acids	645:686	The resultant vinyl C-glycosyl amino acids	645:686	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	2	34	theme	acids	364:368	arg1	syntheses					328:336	the syntheses	324:336	the syntheses of vinyl C-glycosyl amino acids and peptides	324:381	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	2	35	theme	amino	358:362	arg1	acids					364:368	vinyl C-glycosyl amino acids	341:368	vinyl C-glycosyl amino acids	341:368	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	4	36	theme	peptide	812:818	arg1	chains					835:840	the peptide and saccharide chains	808:840	chains	835:840	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	37	theme	C-glycosyl	665:674	arg1	amenable					747:754	amenable	747:754	amenable	747:754	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	37	theme	C-glycosyl	665:674	arg1	acids					682:686	The resultant vinyl C-glycosyl amino acids	645:686	The resultant vinyl C-glycosyl amino acids	645:686	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	3	38	theme	partners	577:584	arg1	scope					555:559	a wide scope	548:559	a wide scope of the coupling partners, including complex oligosaccharide and peptide substrates	548:642	It accommodates a wide scope of the coupling partners, including complex oligosaccharide and peptide substrates.					
34389709	4	39	contain	bear	708:711	arg2	groups					735:740	common O/N-protecting groups	713:740	common O/N-protecting groups	713:740	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	39	contain	bear	708:711	arg1	amenable					747:754	amenable	747:754	amenable	747:754	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	39	contain	bear	708:711	arg1	peptides					692:699	peptides	692:699	peptides	692:699	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	39	contain	bear	708:711	arg1	acids					682:686	The resultant vinyl C-glycosyl amino acids	645:686	The resultant vinyl C-glycosyl amino acids	645:686	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	2	40	theme	C-glycosyl	347:356	arg1	acids					364:368	vinyl C-glycosyl amino acids	341:368	vinyl C-glycosyl amino acids	341:368	Herein, we disclose a protocol for the syntheses of vinyl C-glycosyl amino acids and peptides, employing a nickel-catalyzed reductive hydroglycosylation reaction of alkyne derivatives of amino acids and peptides with common glycosyl bromides.					
34389709	1	41	theme	great	258:262	arg1	potential					278:286	great therapeutical potential	258:286	great therapeutical potential	258:286	C-Glycosyl peptides/proteins are metabolically stable mimics of the native glycopeptides/proteins bearing O/N-glycosidic linkages, and are thus of great therapeutical potential.					
34389709	0	42	theme	Ni-catalyzed	57:68	arg1	hydroglycosylation					80:97	Ni-catalyzed reductive hydroglycosylation	57:97	Ni-catalyzed reductive hydroglycosylation of alkynes	57:108	Facile access to C-glycosyl amino acids and peptides via Ni-catalyzed reductive hydroglycosylation of alkynes.					
34389709	1	43	theme	therapeutical	264:276	arg1	potential					278:286	great therapeutical potential	258:286	great therapeutical potential	258:286	C-Glycosyl peptides/proteins are metabolically stable mimics of the native glycopeptides/proteins bearing O/N-glycosidic linkages, and are thus of great therapeutical potential.					
34389709	4	44	theme	amino	676:680	arg1	amenable					747:754	amenable	747:754	amenable	747:754	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
34389709	4	44	theme	amino	676:680	arg1	acids					682:686	The resultant vinyl C-glycosyl amino acids	645:686	The resultant vinyl C-glycosyl amino acids	645:686	The resultant vinyl C-glycosyl amino acids and peptides, which bear common O/N-protecting groups, are amenable to further transformations, including elongation of the peptide and saccharide chains.					
32737218	9	0	theme	adult	1278:1282	arg1	sex-matching					1284:1295	age-matching and adult sex-matching	1261:1295	age-matching and adult sex-matching	1261:1295	These findings highlight the importance to consider age-matching and adult sex-matching for urinary glycan studies.					
32737218	1	1	theme	adult	148:152	arg1	glycomes					162:169	the pediatric and adult urinary glycomes	130:169	the pediatric and adult urinary glycomes	130:169	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	1	2	theme	=	303:303	arg1	n					301:301	n	301:301	n = 22	301:306	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	1	2	theme	=	303:303	arg1	years					294:298	21-50 years	288:298	21-50 years	288:298	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	1	3	theme	pediatric	246:254	arg1	individuals					309:319	normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals	231:319	normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals	231:319	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	10	4	theme	other	1437:1441	arg1	states					1451:1456	other disease states	1437:1456	other disease states affected by glycosylation	1437:1482	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	8	5	gly	tetrasialylated	1184:1198	arg1	glycans					1200:1206	the high-mannose and tetrasialylated glycans	1163:1206	glycans	1200:1206	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	10	6	theme	normal	1340:1345	arg1	glycomes					1375:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	10	6	theme	normal	1340:1345	arg1	reference					1408:1416	a baseline reference	1397:1416	a baseline reference for comparisons to other disease states affected by glycosylation	1397:1482	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	1	7	theme	urinary	154:160	arg1	glycomes					162:169	the pediatric and adult urinary glycomes	130:169	the pediatric and adult urinary glycomes	130:169	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	6	8	theme	pediatric	940:948	arg1	group					960:964	the pediatric and adult group	936:964	the pediatric and adult group	936:964	We further analyzed gender-associated glycan changes in the pediatric and adult group, respectively.					
32737218	10	9	theme	adult	1361:1365	arg1	glycomes					1375:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	10	9	theme	adult	1361:1365	arg1	reference					1408:1416	a baseline reference	1397:1416	a baseline reference for comparisons to other disease states affected by glycosylation	1397:1482	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	7	10	theme	glycan	1039:1044	arg1	levels					1046:1051	glycan levels	1039:1051	glycan levels	1039:1051	In the pediatric group, there was almost no difference of glycan levels between males and females.					
32737218	10	11	theme	identified	1329:1338	arg1	glycomes					1375:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	10	11	theme	identified	1329:1338	arg1	reference					1408:1416	a baseline reference	1397:1416	a baseline reference for comparisons to other disease states affected by glycosylation	1397:1482	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	3	12	theme	glycan	473:478	arg1	compositions					480:491	the 46 glycan compositions	466:491	the 46 glycan compositions	466:491	We performed quantitative comparisons of the 46 glycan compositions between different age and sex groups.					
32737218	3	13	theme	different	501:509	arg1	groups					523:528	different age and sex groups	501:528	different age and sex groups	501:528	We performed quantitative comparisons of the 46 glycan compositions between different age and sex groups.					
32737218	5	14	theme	higher	679:684	arg1	level					686:690	a higher level	677:690	a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans	677:853	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	1	15	dep	adult	281:285	arg1	n					301:301	n	301:301	n = 22	301:306	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	1	15	dep	adult	281:285	arg1	years					294:298	21-50 years	288:298	21-50 years	288:298	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	7	16	theme	levels	1046:1051	arg1	difference					1025:1034	no difference	1022:1034	no difference of glycan levels between males and females	1022:1077	In the pediatric group, there was almost no difference of glycan levels between males and females.					
32737218	3	17	theme	compositions	480:491	arg1	comparisons					451:461	quantitative comparisons	438:461	quantitative comparisons of the 46 glycan compositions between different age and sex groups	438:528	We performed quantitative comparisons of the 46 glycan compositions between different age and sex groups.					
32737218	9	18	theme	urinary	1301:1307	arg1	studies					1316:1322	urinary glycan studies	1301:1322	urinary glycan studies	1301:1322	These findings highlight the importance to consider age-matching and adult sex-matching for urinary glycan studies.					
32737218	5	19	theme	glycans	803:809	arg1	level					686:690	a higher level	677:690	a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans	677:853	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	8	20	from	males	1136:1140	arg1	abundant					1124:1131	abundant	1124:1131	abundant	1124:1131	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	8	20	from	males	1136:1140	arg1	majority					1094:1101	the majority	1090:1101	the majority of glycans	1090:1112	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	5	21	gly	fucosylated	771:781	arg1	glycans					803:809	neutral fucosylated and agalactosylated glycans	763:809	neutral fucosylated and agalactosylated glycans	763:809	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	2	22	theme	compositions	346:357	arg1	total					324:328	A total	322:328	A total of 116 N-glycan compositions	322:357	A total of 116 N-glycan compositions were identified, and 46 of them could be reproducibly quantified.					
32737218	1	23	theme	glycomes	162:169	arg1	comparison					116:125	comparison	116:125	comparison of the pediatric and adult urinary glycomes	116:169	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	1	23	theme	glycomes	162:169	arg1	characterization					95:110	an in-depth characterization	83:110	an in-depth characterization	83:110	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	5	24	theme	trisialylated	833:845	arg1	glycans					847:853	trisialylated glycans	833:853	trisialylated glycans	833:853	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	25	theme	excluding	748:756	arg1	glycans					739:745	asialylated/afucosylated glycans	714:745	asialylated/afucosylated glycans (excluding HM)	714:760	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	25	theme	excluding	748:756	arg1	HM					758:759	excluding HM	748:759	excluding HM	748:759	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	10	26	theme	urinary	1367:1373	arg1	glycomes					1375:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	10	26	theme	urinary	1367:1373	arg1	reference					1408:1416	a baseline reference	1397:1416	a baseline reference for comparisons to other disease states affected by glycosylation	1397:1482	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	1	27	dep	=	303:303	arg1	22					305:306	22	305:306	22	305:306	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	0	28	theme	in-depth	3:10	arg1	Comparison					12:21	An in-depth Comparison	0:21	An in-depth Comparison of the Pediatric and Adult Urinary N-glycomes	0:67	An in-depth Comparison of the Pediatric and Adult Urinary N-glycomes.					
32737218	8	29	from	abundant	1124:1131	arg1	males					1136:1140	males	1136:1140	males	1136:1140	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	8	29	from	abundant	1124:1131	arg1	adult					1083:1087	adult	1083:1087	adult	1083:1087	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	10	30	theme	baseline	1399:1406	arg1	glycomes					1375:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	10	30	theme	baseline	1399:1406	arg1	reference					1408:1416	a baseline reference	1397:1416	a baseline reference for comparisons to other disease states affected by glycosylation	1397:1482	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	5	31	theme	high-mannose	695:706	arg1	glycans					739:745	asialylated/afucosylated glycans	714:745	asialylated/afucosylated glycans (excluding HM)	714:760	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	31	theme	high-mannose	695:706	arg1	HM					758:759	excluding HM	748:759	excluding HM	748:759	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	31	theme	high-mannose	695:706	arg1	HM					709:710	HM	709:710	HM	709:710	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	3	32	theme	age	511:513	arg1	groups					523:528	different age and sex groups	501:528	different age and sex groups	501:528	We performed quantitative comparisons of the 46 glycan compositions between different age and sex groups.					
32737218	7	33	theme	pediatric	988:996	arg1	group					998:1002	the pediatric group	984:1002	the pediatric group	984:1002	In the pediatric group, there was almost no difference of glycan levels between males and females.					
32737218	5	34	contain	contain	669:675	arg1	N-glycome					646:654	The pediatric urinary N-glycome	624:654	The pediatric urinary N-glycome	624:654	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	34	contain	contain	669:675	arg2	level					686:690	a higher level	677:690	a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans	677:853	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	8	35	from	adult	1083:1087	arg1	abundant					1124:1131	abundant	1124:1131	abundant	1124:1131	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	8	35	from	adult	1083:1087	arg1	majority					1094:1101	the majority	1090:1101	the majority of glycans	1090:1112	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	9	36	theme	glycan	1309:1314	arg1	studies					1316:1322	urinary glycan studies	1301:1322	urinary glycan studies	1301:1322	These findings highlight the importance to consider age-matching and adult sex-matching for urinary glycan studies.					
32737218	8	37	theme	high-mannose	1167:1178	arg1	glycans					1200:1206	the high-mannose and tetrasialylated glycans	1163:1206	glycans	1200:1206	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	6	38	theme	glycan	918:923	arg1	changes					925:931	gender-associated glycan changes	900:931	gender-associated glycan changes	900:931	We further analyzed gender-associated glycan changes in the pediatric and adult group, respectively.					
32737218	10	39	theme	disease	1443:1449	arg1	states					1451:1456	other disease states	1437:1456	other disease states affected by glycosylation	1437:1482	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	10	40	theme	pediatric	1347:1355	arg1	glycomes					1375:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes	1325:1382	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	10	40	theme	pediatric	1347:1355	arg1	reference					1408:1416	a baseline reference	1397:1416	a baseline reference for comparisons to other disease states affected by glycosylation	1397:1482	The identified normal pediatric and adult urinary glycomes can serve as a baseline reference for comparisons to other disease states affected by glycosylation.					
32737218	1	41	theme	in-depth	86:93	arg1	characterization					95:110	an in-depth characterization	83:110	an in-depth characterization	83:110	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	1	42	theme	based	192:196	arg1	method					208:213	a nanoLC-MS/MS based glycomics method	177:213	a nanoLC-MS/MS based glycomics method	177:213	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	6	43	theme	gender-associated	900:916	arg1	changes					925:931	gender-associated glycan changes	900:931	gender-associated glycan changes	900:931	We further analyzed gender-associated glycan changes in the pediatric and adult group, respectively.					
32737218	9	44	theme	age-matching	1261:1272	arg1	sex-matching					1284:1295	age-matching and adult sex-matching	1261:1295	age-matching and adult sex-matching	1261:1295	These findings highlight the importance to consider age-matching and adult sex-matching for urinary glycan studies.					
32737218	6	45	theme	adult	954:958	arg1	group					960:964	the pediatric and adult group	936:964	the pediatric and adult group	936:964	We further analyzed gender-associated glycan changes in the pediatric and adult group, respectively.					
32737218	3	46	theme	quantitative	438:449	arg1	comparisons					451:461	quantitative comparisons	438:461	quantitative comparisons of the 46 glycan compositions between different age and sex groups	438:528	We performed quantitative comparisons of the 46 glycan compositions between different age and sex groups.					
32737218	2	47	theme	them	386:389	arg1	them					386:389	them	386:389	them	386:389	A total of 116 N-glycan compositions were identified, and 46 of them could be reproducibly quantified.					
32737218	2	47	theme	them	386:389	arg1	46					380:381	46	380:381	46	380:381	A total of 116 N-glycan compositions were identified, and 46 of them could be reproducibly quantified.					
32737218	1	48	theme	glycomics	198:206	arg1	method					208:213	a nanoLC-MS/MS based glycomics method	177:213	a nanoLC-MS/MS based glycomics method	177:213	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	0	49	theme	Pediatric	30:38	arg1	N-glycomes					58:67	the Pediatric and Adult Urinary N-glycomes	26:67	the Pediatric and Adult Urinary N-glycomes	26:67	An in-depth Comparison of the Pediatric and Adult Urinary N-glycomes.					
32737218	4	50	theme	pediatric	595:603	arg1	cohorts					615:621	the pediatric and adult cohorts	591:621	the pediatric and adult cohorts	591:621	The results showed significant quantitative changes between the pediatric and adult cohorts.					
32737218	2	51	theme	N-glycan	337:344	arg1	compositions					346:357	116 N-glycan compositions	333:357	116 N-glycan compositions	333:357	A total of 116 N-glycan compositions were identified, and 46 of them could be reproducibly quantified.					
32737218	4	52	theme	adult	609:613	arg1	cohorts					615:621	the pediatric and adult cohorts	591:621	the pediatric and adult cohorts	591:621	The results showed significant quantitative changes between the pediatric and adult cohorts.					
32737218	5	53	theme	asialylated/afucosylated	714:737	arg1	glycans					739:745	asialylated/afucosylated glycans	714:745	asialylated/afucosylated glycans (excluding HM)	714:760	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	53	theme	asialylated/afucosylated	714:737	arg1	HM					758:759	excluding HM	748:759	excluding HM	748:759	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	53	theme	asialylated/afucosylated	714:737	arg1	HM					709:710	HM	709:710	HM	709:710	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	4	54	theme	quantitative	562:573	arg1	changes					575:581	significant quantitative changes	550:581	significant quantitative changes between the pediatric and adult cohorts	550:621	The results showed significant quantitative changes between the pediatric and adult cohorts.					
32737218	5	55	theme	agalactosylated	787:801	arg1	glycans					803:809	neutral fucosylated and agalactosylated glycans	763:809	neutral fucosylated and agalactosylated glycans	763:809	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	0	56	theme	Adult	44:48	arg1	N-glycomes					58:67	the Pediatric and Adult Urinary N-glycomes	26:67	the Pediatric and Adult Urinary N-glycomes	26:67	An in-depth Comparison of the Pediatric and Adult Urinary N-glycomes.					
32737218	5	57	theme	lower	818:822	arg1	level					824:828	a lower level	816:828	a lower level of trisialylated glycans	816:853	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	3	58	theme	sex	519:521	arg1	groups					523:528	different age and sex groups	501:528	different age and sex groups	501:528	We performed quantitative comparisons of the 46 glycan compositions between different age and sex groups.					
32737218	5	59	theme	urinary	638:644	arg1	N-glycome					646:654	The pediatric urinary N-glycome	624:654	The pediatric urinary N-glycome	624:654	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	60	theme	glycans	847:853	arg1	glycans					739:745	asialylated/afucosylated glycans	714:745	asialylated/afucosylated glycans (excluding HM)	714:760	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	60	theme	glycans	847:853	arg1	HM					758:759	excluding HM	748:759	excluding HM	748:759	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	60	theme	glycans	847:853	arg1	HM					709:710	HM	709:710	HM	709:710	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	60	theme	glycans	847:853	arg1	level					824:828	a lower level	816:828	a lower level of trisialylated glycans	816:853	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	60	theme	glycans	847:853	arg1	glycans					803:809	neutral fucosylated and agalactosylated glycans	763:809	neutral fucosylated and agalactosylated glycans	763:809	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	1	61	theme	adult	281:285	arg1	individuals					309:319	normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals	231:319	normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals	231:319	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	5	62	theme	pediatric	628:636	arg1	N-glycome					646:654	The pediatric urinary N-glycome	624:654	The pediatric urinary N-glycome	624:654	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	8	63	theme	glycans	1106:1112	arg1	abundant					1124:1131	abundant	1124:1131	abundant	1124:1131	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	8	63	theme	glycans	1106:1112	arg1	majority					1094:1101	the majority	1090:1101	the majority of glycans	1090:1112	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	5	64	gly	trisialylated	833:845	arg1	glycans					847:853	trisialylated glycans	833:853	trisialylated glycans	833:853	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	65	theme	glycans	739:745	arg1	level					686:690	a higher level	677:690	a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans	677:853	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	66	gly	asialylated/afucosylated	714:737	arg1	glycans					739:745	asialylated/afucosylated glycans	714:745	asialylated/afucosylated glycans (excluding HM)	714:760	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	66	gly	asialylated/afucosylated	714:737	arg1	HM					758:759	excluding HM	748:759	excluding HM	748:759	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	5	66	gly	asialylated/afucosylated	714:737	arg1	HM					709:710	HM	709:710	HM	709:710	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	1	67	dep	pediatric	246:254	arg1	=					271:271	=	271:271	=	271:271	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	5	68	theme	fucosylated	771:781	arg1	glycans					803:809	neutral fucosylated and agalactosylated glycans	763:809	neutral fucosylated and agalactosylated glycans	763:809	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	0	69	theme	N-glycomes	58:67	arg1	Comparison					12:21	An in-depth Comparison	0:21	An in-depth Comparison of the Pediatric and Adult Urinary N-glycomes	0:67	An in-depth Comparison of the Pediatric and Adult Urinary N-glycomes.					
32737218	8	70	theme	tetrasialylated	1184:1198	arg1	glycans					1200:1206	the high-mannose and tetrasialylated glycans	1163:1206	glycans	1200:1206	In adult, the majority of glycans were more abundant in males than females, except the high-mannose and tetrasialylated glycans.					
32737218	5	71	theme	neutral	763:769	arg1	glycans					803:809	neutral fucosylated and agalactosylated glycans	763:809	neutral fucosylated and agalactosylated glycans	763:809	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	1	72	theme	pediatric	134:142	arg1	glycomes					162:169	the pediatric and adult urinary glycomes	130:169	the pediatric and adult urinary glycomes	130:169	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	1	73	theme	normal	231:236	arg1	individuals					309:319	normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals	231:319	normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals	231:319	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	0	74	theme	Urinary	50:56	arg1	N-glycomes					58:67	the Pediatric and Adult Urinary N-glycomes	26:67	the Pediatric and Adult Urinary N-glycomes	26:67	An in-depth Comparison of the Pediatric and Adult Urinary N-glycomes.					
32737218	1	75	dep	=	271:271	arg1	years					262:266	1-10 years	257:266	1-10 years	257:266	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
32737218	4	76	theme	significant	550:560	arg1	changes					575:581	significant quantitative changes	550:581	significant quantitative changes between the pediatric and adult cohorts	550:621	The results showed significant quantitative changes between the pediatric and adult cohorts.					
32737218	5	77	theme	level	824:828	arg1	level					686:690	a higher level	677:690	a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans	677:853	The pediatric urinary N-glycome was found to contain a higher level of high-mannose (HM), asialylated/afucosylated glycans (excluding HM), neutral fucosylated and agalactosylated glycans, and a lower level of trisialylated glycans compared with the adult.					
32737218	1	78	theme	healthy	238:244	arg1	individuals					309:319	normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals	231:319	normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals	231:319	We performed an in-depth characterization and comparison of the pediatric and adult urinary glycomes using a nanoLC-MS/MS based glycomics method, which included normal healthy pediatric (1-10 years, n = 21) and adult (21-50 years, n = 22) individuals.					
34669426	5	0	dep	C.	978:979	arg1	revealed					1028:1035	revealed	1028:1035	revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response	1028:1157	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	14	1	theme	glycan	2097:2102	arg1	holes					2104:2108	the glycan holes	2093:2108	the glycan holes	2093:2108	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	7	2	theme	located	1383:1389	arg1	target					1284:1289	The sole target	1275:1289	The sole target for antibodies that can neutralize the virus	1275:1334	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	7	2	theme	located	1383:1389	arg1	Envs					1377:1380	Envs	1377:1380	Envs	1377:1380	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	7	2	theme	located	1383:1389	arg1	glycoproteins					1362:1374	the trimeric envelope glycoproteins	1340:1374	the trimeric envelope glycoproteins (Envs) located on the viral surface	1340:1410	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	4	3	theme	stronger	824:831	arg1	responses					848:856	stronger autologous NAb responses	824:856	stronger autologous NAb responses	824:856	Specifically, when we knocked out the N130 and N289 glycans, which are absent from the well-characterized B41 SOSIP trimer, we observed stronger autologous NAb responses.					
34669426	6	4	theme	first	1193:1197	arg1	description					1199:1209	the first description	1189:1209	the first description of HIV-1	1189:1218	IMPORTANCE Forty years after the first description of HIV-1, the search for a protective vaccine is still ongoing.					
34669426	13	5	theme	strain-specific	1860:1874	arg1	responses					1898:1906	strain-specific neutralizing antibody responses	1860:1906	strain-specific neutralizing antibody responses	1860:1906	First, we show that strain-specific neutralizing antibody responses can be increased by creating artificial holes in the glycan shield.					
34669426	4	6	theme	B41	794:796	arg1	trimer					804:809	the well-characterized B41 SOSIP trimer	771:809	the well-characterized B41 SOSIP trimer	771:809	Specifically, when we knocked out the N130 and N289 glycans, which are absent from the well-characterized B41 SOSIP trimer, we observed stronger autologous NAb responses.					
34669426	14	7	theme	surface	2077:2083	arg1	area					2085:2088	the surface area	2073:2088	the surface area of the glycan holes	2073:2108	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	3	8	theme	SOSIP	673:677	arg1	trimers					679:685	the AMC011 and AMC016 SOSIP trimers	651:685	trimers	679:685	Here, we sought to gain a deeper understanding of the autologous neutralization by introducing holes in the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers.					
34669426	1	9	gly	glycoprotein	184:195	arg1	Env					198:200	Env	198:200	Env	198:200	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	1	9	gly	glycoprotein	184:195	arg1	glycoprotein					184:195	trimeric envelope glycoprotein	166:195	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env)	118:201	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	10	10	theme	Immunized	1631:1639	arg1	animals					1641:1647	Immunized animals	1631:1647	Immunized animals	1631:1647	Immunized animals developed antibodies against such glycan holes.					
34669426	5	11	dep	subtypes	959:966	arg1	C.					978:979	C.	978:979	C.	978:979	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	5	11	dep	subtypes	959:966	arg1	A					968:968	A	968:968	A	968:968	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	5	11	dep	subtypes	959:966	arg1	subtypes					959:966	subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response	959:1157	subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response	959:1157	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	5	11	dep	subtypes	959:966	arg1	B					971:971	B	971:971	B	971:971	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	6	12	theme	IMPORTANCE	1160:1169	arg1	Forty years					1171:1181	IMPORTANCE Forty years	1160:1181	IMPORTANCE Forty years	1160:1181	IMPORTANCE Forty years after the first description of HIV-1, the search for a protective vaccine is still ongoing.					
34669426	10	13	theme	glycan	1683:1688	arg1	holes					1690:1694	such glycan holes	1678:1694	such glycan holes	1678:1694	Immunized animals developed antibodies against such glycan holes.					
34669426	1	14	theme	envelope	175:182	arg1	Env					198:200	Env	198:200	Env	198:200	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	1	14	theme	envelope	175:182	arg1	glycoprotein					184:195	trimeric envelope glycoprotein	166:195	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env)	118:201	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	9	15	theme	strains	1590:1596	arg1	trimers					1568:1574	the Env trimers	1560:1574	the Env trimers of some viral strains	1560:1596	However, the Env trimers of some viral strains have holes in the glycan shield.					
34669426	8	16	from	recognition	1517:1527	arg1	components					1501:1510	the underlying protein components	1478:1510	the underlying protein components from recognition by the immune system	1478:1548	The glycoprotein surface is covered with glycans that shield off the underlying protein components from recognition by the immune system.					
34669426	5	17	from	subtypes	959:966	arg1	trimers					946:952	diverse SOSIP trimers	932:952	diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response	932:1157	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	4	18	theme	NAb	844:846	arg1	responses					848:856	stronger autologous NAb responses	824:856	stronger autologous NAb responses	824:856	Specifically, when we knocked out the N130 and N289 glycans, which are absent from the well-characterized B41 SOSIP trimer, we observed stronger autologous NAb responses.					
34669426	7	19	theme	sole	1279:1282	arg1	target					1284:1289	The sole target	1275:1289	The sole target for antibodies that can neutralize the virus	1275:1334	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	7	19	theme	sole	1279:1282	arg1	glycoproteins					1362:1374	the trimeric envelope glycoproteins	1340:1374	the trimeric envelope glycoproteins (Envs) located on the viral surface	1340:1410	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	8	20	dep	shield	1467:1472	arg1	off					1474:1476	off	1474:1476	off	1474:1476	The glycoprotein surface is covered with glycans that shield off the underlying protein components from recognition by the immune system.					
34669426	1	21	dep	glycan	245:250	arg1	shield					252:257	shield	252:257	shield	252:257	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	1	22	theme	immunodeficiency	128:143	arg1	type					151:154	The human immunodeficiency virus type 1	118:156	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env)	118:201	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	1	22	theme	immunodeficiency	128:143	arg1	HIV-1					159:163	HIV-1	159:163	HIV-1	159:163	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	2	23	theme	antibody	483:490	arg1	responses					498:506	strain-specific, autologous neutralizing antibody (NAb) responses	442:506	strain-specific, autologous neutralizing antibody (NAb) responses	442:506	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	1	24	theme	antibody	310:317	arg1	recognition					319:329	antibody recognition	310:329	antibody recognition	310:329	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	3	25	theme	neutralization	574:587	arg1	understanding					542:554	a deeper understanding	533:554	a deeper understanding of the autologous neutralization	533:587	Here, we sought to gain a deeper understanding of the autologous neutralization by introducing holes in the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers.					
34669426	4	26	dep	N130	726:729	arg1	the					722:724	the	722:724	the	722:724	Specifically, when we knocked out the N130 and N289 glycans, which are absent from the well-characterized B41 SOSIP trimer, we observed stronger autologous NAb responses.					
34669426	4	26	dep	N130	726:729	arg1	glycans					740:746	glycans	740:746	glycans	740:746	Specifically, when we knocked out the N130 and N289 glycans, which are absent from the well-characterized B41 SOSIP trimer, we observed stronger autologous NAb responses.					
34669426	2	27	gly	glycosylation	400:412	arg2	sites					414:418	missing potential N-linked glycosylation sites	373:418	missing potential N-linked glycosylation sites (PNGS)	373:425	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	2	27	gly	glycosylation	400:412	arg2	PNGS					421:424	PNGS	421:424	PNGS	421:424	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	4	28	from	trimer	804:809	arg1	absent					759:764	absent	759:764	absent	759:764	Specifically, when we knocked out the N130 and N289 glycans, which are absent from the well-characterized B41 SOSIP trimer, we observed stronger autologous NAb responses.					
34669426	12	29	theme	immune	1822:1827	arg1	responses					1829:1837	these specific immune responses	1807:1837	these specific immune responses	1807:1837	Here, we sought to gain a deeper understanding of what drives these specific immune responses.					
34669426	8	30	theme	glycoprotein	1417:1428	arg1	surface					1430:1436	The glycoprotein surface	1413:1436	The glycoprotein surface	1413:1436	The glycoprotein surface is covered with glycans that shield off the underlying protein components from recognition by the immune system.					
34669426	14	31	theme	diverse	2000:2006	arg1	set					2008:2010	a diverse set	1998:2010	a diverse set of Env trimers with different characteristics	1998:2056	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	14	31	theme	diverse	2000:2006	arg1	trimers					2019:2025	Env trimers	2015:2025	Env trimers with different characteristics	2015:2056	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	14	32	theme	antibodies	2183:2192	arg1	induction					2141:2149	the induction	2137:2149	the induction of strain-specific neutralizing antibodies	2137:2192	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	2	33	theme	missing	373:379	arg1	PNGS					421:424	PNGS	421:424	PNGS	421:424	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	2	33	theme	missing	373:379	arg1	sites					414:418	missing potential N-linked glycosylation sites	373:418	missing potential N-linked glycosylation sites (PNGS)	373:425	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	9	34	dep	glycan	1616:1621	arg1	shield					1623:1628	shield	1623:1628	shield	1623:1628	However, the Env trimers of some viral strains have holes in the glycan shield.					
34669426	7	35	gly	glycoproteins	1362:1374	arg1	target					1284:1289	The sole target	1275:1289	The sole target for antibodies that can neutralize the virus	1275:1334	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	7	35	gly	glycoproteins	1362:1374	arg1	Envs					1377:1380	Envs	1377:1380	Envs	1377:1380	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	7	35	gly	glycoproteins	1362:1374	arg1	glycoproteins					1362:1374	the trimeric envelope glycoproteins	1340:1374	the trimeric envelope glycoproteins (Envs) located on the viral surface	1340:1410	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	14	36	theme	strain-specific	2154:2168	arg1	antibodies					2183:2192	strain-specific neutralizing antibodies	2154:2192	strain-specific neutralizing antibodies	2154:2192	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	5	37	theme	glycan	1085:1090	arg1	holes					1092:1096	glycan holes	1085:1096	glycan holes	1085:1096	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	5	38	theme	variable	887:894	arg1	responses					900:908	the highly variable NAb responses	876:908	the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response	876:1157	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	0	39	theme	Hole	11:14	arg1	Area					16:19	The Glycan Hole Area	0:19	The Glycan Hole Area of HIV-1 Envelope Trimers	0:45	The Glycan Hole Area of HIV-1 Envelope Trimers Contributes Prominently to the Induction of Autologous Neutralization.					
34669426	1	40	theme	virus	145:149	arg1	type					151:154	The human immunodeficiency virus type 1	118:156	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env)	118:201	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	1	40	theme	virus	145:149	arg1	HIV-1					159:163	HIV-1	159:163	HIV-1	159:163	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	2	41	theme	strain-specific	442:456	arg1	responses					498:506	strain-specific, autologous neutralizing antibody (NAb) responses	442:506	strain-specific, autologous neutralizing antibody (NAb) responses	442:506	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	2	42	theme	N-linked	391:398	arg1	PNGS					421:424	PNGS	421:424	PNGS	421:424	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	2	42	theme	N-linked	391:398	arg1	sites					414:418	missing potential N-linked glycosylation sites	373:418	missing potential N-linked glycosylation sites (PNGS)	373:425	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	1	43	theme	underlying	277:286	arg1	surface					297:303	the underlying peptidic surface	273:303	the underlying peptidic surface	273:303	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	13	44	dep	glycan	1961:1966	arg1	shield					1968:1973	shield	1968:1973	shield	1968:1973	First, we show that strain-specific neutralizing antibody responses can be increased by creating artificial holes in the glycan shield.					
34669426	0	45	theme	Envelope	30:37	arg1	Trimers					39:45	HIV-1 Envelope Trimers	24:45	HIV-1 Envelope Trimers	24:45	The Glycan Hole Area of HIV-1 Envelope Trimers Contributes Prominently to the Induction of Autologous Neutralization.					
34669426	8	46	theme	underlying	1482:1491	arg1	components					1501:1510	the underlying protein components	1478:1510	the underlying protein components from recognition by the immune system	1478:1548	The glycoprotein surface is covered with glycans that shield off the underlying protein components from recognition by the immune system.					
34669426	13	47	theme	artificial	1937:1946	arg1	holes					1948:1952	artificial holes	1937:1952	artificial holes	1937:1952	First, we show that strain-specific neutralizing antibody responses can be increased by creating artificial holes in the glycan shield.					
34669426	9	48	contain	have	1598:1601	arg1	trimers					1568:1574	the Env trimers	1560:1574	the Env trimers of some viral strains	1560:1596	However, the Env trimers of some viral strains have holes in the glycan shield.					
34669426	9	48	contain	have	1598:1601	arg2	holes					1603:1607	holes	1603:1607	holes	1603:1607	However, the Env trimers of some viral strains have holes in the glycan shield.					
34669426	5	49	theme	autologous	1135:1144	arg1	response					1150:1157	the autologous NAb response	1131:1157	the autologous NAb response	1131:1157	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	7	50	theme	viral	1398:1402	arg1	surface					1404:1410	the viral surface	1394:1410	the viral surface	1394:1410	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	5	51	theme	SOSIP	940:944	arg1	trimers					946:952	diverse SOSIP trimers	932:952	diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response	932:1157	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	2	52	theme	potential	381:389	arg1	PNGS					421:424	PNGS	421:424	PNGS	421:424	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	2	52	theme	potential	381:389	arg1	sites					414:418	missing potential N-linked glycosylation sites	373:418	missing potential N-linked glycosylation sites (PNGS)	373:425	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	2	53	theme	glycans	357:363	arg1	absence					336:342	The absence	332:342	The absence of conserved glycans	332:363	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	5	54	theme	response	1150:1157	arg1	magnitude					1118:1126	the magnitude	1114:1126	the magnitude of the autologous NAb response	1114:1157	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	14	55	theme	holes	2104:2108	arg1	area					2085:2088	the surface area	2073:2088	the surface area of the glycan holes	2073:2108	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	3	56	theme	AMC016	666:671	arg1	trimers					679:685	the AMC011 and AMC016 SOSIP trimers	651:685	trimers	679:685	Here, we sought to gain a deeper understanding of the autologous neutralization by introducing holes in the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers.					
34669426	6	57	theme	HIV-1	1214:1218	arg1	description					1199:1209	the first description	1189:1209	the first description of HIV-1	1189:1218	IMPORTANCE Forty years after the first description of HIV-1, the search for a protective vaccine is still ongoing.					
34669426	14	58	theme	trimers	2019:2025	arg1	set					2008:2010	a diverse set	1998:2010	a diverse set of Env trimers with different characteristics	1998:2056	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	14	58	theme	trimers	2019:2025	arg1	trimers					2019:2025	Env trimers	2015:2025	Env trimers with different characteristics	2015:2056	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	3	59	theme	AMC011	655:660	arg1	shields					640:646	the otherwise dense glycan shields	613:646	the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers	613:685	Here, we sought to gain a deeper understanding of the autologous neutralization by introducing holes in the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers.					
34669426	1	60	theme	dense	239:243	arg1	glycan					245:250	a dense glycan shield	237:257	a dense glycan shield that protects the underlying peptidic surface from antibody recognition	237:329	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	9	61	theme	Env	1564:1566	arg1	trimers					1568:1574	the Env trimers	1560:1574	the Env trimers of some viral strains	1560:1596	However, the Env trimers of some viral strains have holes in the glycan shield.					
34669426	7	62	theme	trimeric	1344:1351	arg1	target					1284:1289	The sole target	1275:1289	The sole target for antibodies that can neutralize the virus	1275:1334	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	7	62	theme	trimeric	1344:1351	arg1	Envs					1377:1380	Envs	1377:1380	Envs	1377:1380	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	7	62	theme	trimeric	1344:1351	arg1	glycoproteins					1362:1374	the trimeric envelope glycoproteins	1340:1374	the trimeric envelope glycoproteins (Envs) located on the viral surface	1340:1410	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	13	63	theme	neutralizing	1876:1887	arg1	responses					1898:1906	strain-specific neutralizing antibody responses	1860:1906	strain-specific neutralizing antibody responses	1860:1906	First, we show that strain-specific neutralizing antibody responses can be increased by creating artificial holes in the glycan shield.					
34669426	7	64	theme	envelope	1353:1360	arg1	target					1284:1289	The sole target	1275:1289	The sole target for antibodies that can neutralize the virus	1275:1334	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	7	64	theme	envelope	1353:1360	arg1	Envs					1377:1380	Envs	1377:1380	Envs	1377:1380	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	7	64	theme	envelope	1353:1360	arg1	glycoproteins					1362:1374	the trimeric envelope glycoproteins	1340:1374	the trimeric envelope glycoproteins (Envs) located on the viral surface	1340:1410	The sole target for antibodies that can neutralize the virus are the trimeric envelope glycoproteins (Envs) located on the viral surface.					
34669426	4	65	theme	well-characterized	775:792	arg1	trimer					804:809	the well-characterized B41 SOSIP trimer	771:809	the well-characterized B41 SOSIP trimer	771:809	Specifically, when we knocked out the N130 and N289 glycans, which are absent from the well-characterized B41 SOSIP trimer, we observed stronger autologous NAb responses.					
34669426	3	66	theme	trimers	679:685	arg1	shields					640:646	the otherwise dense glycan shields	613:646	the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers	613:685	Here, we sought to gain a deeper understanding of the autologous neutralization by introducing holes in the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers.					
34669426	1	67	theme	trimeric	166:173	arg1	Env					198:200	Env	198:200	Env	198:200	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	1	67	theme	trimeric	166:173	arg1	glycoprotein					184:195	trimeric envelope glycoprotein	166:195	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env)	118:201	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	4	68	theme	SOSIP	798:802	arg1	trimer					804:809	the well-characterized B41 SOSIP trimer	771:809	the well-characterized B41 SOSIP trimer	771:809	Specifically, when we knocked out the N130 and N289 glycans, which are absent from the well-characterized B41 SOSIP trimer, we observed stronger autologous NAb responses.					
34669426	10	69	theme	such	1678:1681	arg1	holes					1690:1694	such glycan holes	1678:1694	such glycan holes	1678:1694	Immunized animals developed antibodies against such glycan holes.					
34669426	6	70	theme	protective	1238:1247	arg1	vaccine					1249:1255	a protective vaccine	1236:1255	a protective vaccine	1236:1255	IMPORTANCE Forty years after the first description of HIV-1, the search for a protective vaccine is still ongoing.					
34669426	9	71	theme	viral	1584:1588	arg1	strains					1590:1596	some viral strains	1579:1596	some viral strains	1579:1596	However, the Env trimers of some viral strains have holes in the glycan shield.					
34669426	8	72	theme	immune	1536:1541	arg1	system					1543:1548	the immune system	1532:1548	the immune system	1532:1548	The glycoprotein surface is covered with glycans that shield off the underlying protein components from recognition by the immune system.					
34669426	14	73	theme	different	2032:2040	arg1	characteristics					2042:2056	different characteristics	2032:2056	different characteristics	2032:2056	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	5	74	theme	linear	1009:1014	arg1	regression					1016:1025	linear regression	1009:1025	linear regression	1009:1025	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	3	75	theme	deeper	535:540	arg1	understanding					542:554	a deeper understanding	533:554	a deeper understanding of the autologous neutralization	533:587	Here, we sought to gain a deeper understanding of the autologous neutralization by introducing holes in the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers.					
34669426	4	76	theme	autologous	833:842	arg1	responses					848:856	stronger autologous NAb responses	824:856	stronger autologous NAb responses	824:856	Specifically, when we knocked out the N130 and N289 glycans, which are absent from the well-characterized B41 SOSIP trimer, we observed stronger autologous NAb responses.					
34669426	1	77	theme	human	122:126	arg1	type					151:154	The human immunodeficiency virus type 1	118:156	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env)	118:201	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	1	77	theme	human	122:126	arg1	HIV-1					159:163	HIV-1	159:163	HIV-1	159:163	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	5	78	theme	Statistical	981:991	arg1	analysis					993:1000	Statistical analysis	981:1000	Statistical analysis	981:1000	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	3	79	theme	autologous	563:572	arg1	neutralization					574:587	the autologous neutralization	559:587	the autologous neutralization	559:587	Here, we sought to gain a deeper understanding of the autologous neutralization by introducing holes in the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers.					
34669426	8	80	gly	glycoprotein	1417:1428	arg1	glycoprotein					1417:1428	The glycoprotein surface	1413:1436	The glycoprotein surface	1413:1436	The glycoprotein surface is covered with glycans that shield off the underlying protein components from recognition by the immune system.					
34669426	2	81	theme	neutralizing	470:481	arg1	NAb					493:495	NAb	493:495	NAb	493:495	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	2	81	theme	neutralizing	470:481	arg1	antibody					483:490	neutralizing antibody	470:490	strain-specific, autologous neutralizing antibody (NAb) responses	442:506	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	5	82	theme	cumulative	1046:1055	arg1	area					1057:1060	the cumulative area	1042:1060	the cumulative area exposed on a trimer by glycan holes	1042:1096	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	12	83	theme	specific	1813:1820	arg1	responses					1829:1837	these specific immune responses	1807:1837	these specific immune responses	1807:1837	Here, we sought to gain a deeper understanding of what drives these specific immune responses.					
34669426	14	84	theme	Env	2015:2017	arg1	trimers					2019:2025	Env trimers	2015:2025	Env trimers with different characteristics	2015:2056	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	2	85	dep	strain-specific	442:456	arg1	autologous					459:468	autologous	459:468	autologous	459:468	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	0	86	theme	Neutralization	102:115	arg1	Induction					78:86	the Induction	74:86	the Induction of Autologous Neutralization	74:115	The Glycan Hole Area of HIV-1 Envelope Trimers Contributes Prominently to the Induction of Autologous Neutralization.					
34669426	1	87	gly	glycosylated	214:225	arg1	type					151:154	The human immunodeficiency virus type 1	118:156	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env)	118:201	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	1	87	gly	glycosylated	214:225	arg1	HIV-1					159:163	HIV-1	159:163	HIV-1	159:163	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	14	88	theme	neutralizing	2170:2181	arg1	antibodies					2183:2192	strain-specific neutralizing antibodies	2154:2192	strain-specific neutralizing antibodies	2154:2192	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	0	89	theme	Glycan	4:9	arg1	Area					16:19	The Glycan Hole Area	0:19	The Glycan Hole Area of HIV-1 Envelope Trimers	0:45	The Glycan Hole Area of HIV-1 Envelope Trimers Contributes Prominently to the Induction of Autologous Neutralization.					
34669426	3	90	theme	glycan	633:638	arg1	shields					640:646	the otherwise dense glycan shields	613:646	the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers	613:685	Here, we sought to gain a deeper understanding of the autologous neutralization by introducing holes in the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers.					
34669426	5	91	theme	NAb	896:898	arg1	responses					900:908	the highly variable NAb responses	876:908	the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response	876:1157	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	1	92	dep	type	151:154	arg1	Env					198:200	Env	198:200	Env	198:200	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	1	92	dep	type	151:154	arg1	glycoprotein					184:195	trimeric envelope glycoprotein	166:195	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env)	118:201	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	2	93	theme	glycosylation	400:412	arg1	PNGS					421:424	PNGS	421:424	PNGS	421:424	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	2	93	theme	glycosylation	400:412	arg1	sites					414:418	missing potential N-linked glycosylation sites	373:418	missing potential N-linked glycosylation sites (PNGS)	373:425	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	0	94	theme	HIV-1	24:28	arg1	Trimers					39:45	HIV-1 Envelope Trimers	24:45	HIV-1 Envelope Trimers	24:45	The Glycan Hole Area of HIV-1 Envelope Trimers Contributes Prominently to the Induction of Autologous Neutralization.					
34669426	12	95	theme	deeper	1771:1776	arg1	understanding					1778:1790	a deeper understanding	1769:1790	a deeper understanding of what drives these specific immune responses	1769:1837	Here, we sought to gain a deeper understanding of what drives these specific immune responses.					
34669426	0	96	theme	Autologous	91:100	arg1	Neutralization					102:115	Autologous Neutralization	91:115	Autologous Neutralization	91:115	The Glycan Hole Area of HIV-1 Envelope Trimers Contributes Prominently to the Induction of Autologous Neutralization.					
34669426	0	97	theme	Trimers	39:45	arg1	Area					16:19	The Glycan Hole Area	0:19	The Glycan Hole Area of HIV-1 Envelope Trimers	0:45	The Glycan Hole Area of HIV-1 Envelope Trimers Contributes Prominently to the Induction of Autologous Neutralization.					
34669426	1	98	theme	peptidic	288:295	arg1	surface					297:303	the underlying peptidic surface	273:303	the underlying peptidic surface	273:303	The human immunodeficiency virus type 1 (HIV-1) trimeric envelope glycoprotein (Env) is heavily glycosylated, creating a dense glycan shield that protects the underlying peptidic surface from antibody recognition.					
34669426	8	99	theme	protein	1493:1499	arg1	components					1501:1510	the underlying protein components	1478:1510	the underlying protein components from recognition by the immune system	1478:1548	The glycoprotein surface is covered with glycans that shield off the underlying protein components from recognition by the immune system.					
34669426	2	100	link	N-linked	391:398	arg1	PNGS					421:424	PNGS	421:424	PNGS	421:424	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	2	100	link	N-linked	391:398	arg1	sites					414:418	missing potential N-linked glycosylation sites	373:418	missing potential N-linked glycosylation sites (PNGS)	373:425	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
34669426	14	101	with	trimers	2019:2025	arg1	characteristics					2042:2056	different characteristics	2032:2056	different characteristics	2032:2056	Second, when studying a diverse set of Env trimers with different characteristics, we found that the surface area of the glycan holes contributes prominently to the induction of strain-specific neutralizing antibodies.					
34669426	5	102	theme	diverse	932:938	arg1	trimers					946:952	diverse SOSIP trimers	932:952	diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response	932:1157	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	3	103	theme	dense	627:631	arg1	shields					640:646	the otherwise dense glycan shields	613:646	the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers	613:685	Here, we sought to gain a deeper understanding of the autologous neutralization by introducing holes in the otherwise dense glycan shields of the AMC011 and AMC016 SOSIP trimers.					
34669426	13	104	theme	antibody	1889:1896	arg1	responses					1898:1906	strain-specific neutralizing antibody responses	1860:1906	strain-specific neutralizing antibody responses	1860:1906	First, we show that strain-specific neutralizing antibody responses can be increased by creating artificial holes in the glycan shield.					
34669426	5	105	theme	NAb	1146:1148	arg1	response					1150:1157	the autologous NAb response	1131:1157	the autologous NAb response	1131:1157	We also analyzed the highly variable NAb responses induced in rabbits by diverse SOSIP trimers from subtypes A, B, and C. Statistical analysis, using linear regression, revealed that the cumulative area exposed on a trimer by glycan holes correlates with the magnitude of the autologous NAb response.					
34669426	6	106	dep	Forty years	1171:1181	arg1	description					1199:1209	the first description	1189:1209	the first description of HIV-1	1189:1218	IMPORTANCE Forty years after the first description of HIV-1, the search for a protective vaccine is still ongoing.					
34669426	2	107	theme	conserved	347:355	arg1	glycans					357:363	conserved glycans	347:363	conserved glycans	347:363	The absence of conserved glycans, due to missing potential N-linked glycosylation sites (PNGS), can result in strain-specific, autologous neutralizing antibody (NAb) responses.					
32045434	8	0	theme	mass	1120:1123	arg1	MS					1139:1140	MS	1139:1140	MS	1139:1140	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	0	theme	mass	1120:1123	arg1	spectrometry					1125:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry	1049:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1049:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	10	1	theme	type	1476:1479	arg1	N-glycans					1481:1489	bisected type N-glycans	1467:1489	bisected type N-glycans	1467:1489	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	12	2	theme	fewer	1684:1688	arg1	glycoforms					1690:1699	fewer glycoforms	1684:1699	fewer glycoforms which constituted a subset of those found in normal pregnancy	1684:1761	GTD-hCG had fewer glycoforms which constituted a subset of those found in normal pregnancy.					
32045434	14	3	contain	had	1906:1908	arg2	signals					1920:1926	increased signals	1910:1926	increased signals for sialylated structures	1910:1952	In terms of terminal epitopes, GTD-hCG had increased signals for sialylated structures, while LewisX antigens were of very minor abundance.					
32045434	14	3	contain	had	1906:1908	arg1	GTD-hCG					1898:1904	GTD-hCG	1898:1904	GTD-hCG	1898:1904	In terms of terminal epitopes, GTD-hCG had increased signals for sialylated structures, while LewisX antigens were of very minor abundance.					
32045434	2	4	theme	biological	268:277	arg1	activity					279:286	its biological activity	264:286	its biological activity	264:286	Glycosylation of hCG is known to be essential for its biological activity.					
32045434	10	5	theme	hCG	1504:1506	arg1	samples					1508:1514	pregnancy hCG samples	1494:1514	pregnancy hCG samples	1494:1514	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	11	6	theme	novel	1582:1586	arg1	observation					1588:1598	A second novel observation	1573:1598	A second novel observation	1573:1598	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	11	6	theme	novel	1582:1586	arg1	presence					1608:1615	the presence	1604:1615	the presence of abundant LewisX antigens on the bisected N-glycans	1604:1669	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	8	7	theme	MS/MS	1147:1151	arg1	methods					1153:1159	MS/MS methods	1147:1159	MS/MS methods	1147:1159	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	7	theme	MS/MS	1147:1151	arg1	spectrometry					1125:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry	1049:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1049:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	8	theme	individual	1229:1238	arg1	samples					1240:1246	the individual samples	1225:1246	the individual samples	1225:1246	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	18	9	theme	cell	2497:2500	arg1	cytotoxicity					2502:2513	NK cell cytotoxicity	2494:2513	NK cell cytotoxicity	2494:2513	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	5	10	theme	hCG	821:823	arg1	identity					791:798	the identity	787:798	the identity of hyperglycosylated hCG	787:823	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	2	11	theme	hCG	231:233	arg1	Glycosylation					214:226	Glycosylation	214:226	Glycosylation of hCG	214:233	Glycosylation of hCG is known to be essential for its biological activity.					
32045434	7	12	theme	gestational	966:976	arg1	GTD					1001:1003	GTD	1001:1003	GTD	1001:1003	hCG was also isolated in urine from women diagnosed with gestational trophoblastic disease (GTD).					
32045434	7	12	theme	gestational	966:976	arg1	disease					992:998	gestational trophoblastic disease	966:998	gestational trophoblastic disease (GTD)	966:1004	hCG was also isolated in urine from women diagnosed with gestational trophoblastic disease (GTD).					
32045434	15	13	theme	different	2066:2074	arg1	proportions					2076:2086	different proportions	2066:2086	different proportions	2066:2086	hCG carries the same N-glycans throughout pregnancy but in different proportions.					
32045434	3	14	theme	Hyperglycosylated	290:306	arg1	variants					309:316	"Hyperglycosylated" variants	289:316	"Hyperglycosylated" variants secreted during early pregnancy	289:348	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	3	14	theme	Hyperglycosylated	290:306	arg1	marker					452:457	a potential diagnostic marker	429:457	a potential diagnostic marker for gestational diseases	429:482	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	10	15	theme	lower	1544:1548	arg1	levels					1550:1555	much lower levels	1539:1555	much lower levels in GTD samples	1539:1570	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	5	16	theme	early	667:671	arg1	pregnancy					682:690	early and late pregnancy	667:690	early and late pregnancy	667:690	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	18	17	theme	bisected	2322:2329	arg1	glycans					2336:2342	bisected type glycans	2322:2342	bisected type glycans	2322:2342	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	14	18	theme	increased	1910:1918	arg1	signals					1920:1926	increased signals	1910:1926	increased signals for sialylated structures	1910:1952	In terms of terminal epitopes, GTD-hCG had increased signals for sialylated structures, while LewisX antigens were of very minor abundance.					
32045434	5	19	theme	late	677:680	arg1	pregnancy					682:690	early and late pregnancy	667:690	early and late pregnancy	667:690	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	10	20	attach	present	1528:1534	arg2	which					1517:1521	which	1517:1521	which	1517:1521	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	10	20	attach	present	1528:1534	arg1	levels					1550:1555	much lower levels	1539:1555	much lower levels in GTD samples	1539:1570	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	1	21	theme	glycoprotein	141:152	arg1	hormone					154:160	a glycoprotein hormone	139:160	a glycoprotein hormone that is essential for the maintenance of pregnancy	139:211	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	1	21	theme	glycoprotein	141:152	arg1	essential					170:178	essential	170:178	essential	170:178	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	1	21	theme	glycoprotein	141:152	arg1	gonadotropin					117:128	Human chorionic gonadotropin	101:128	Human chorionic gonadotropin (hCG)	101:134	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	10	22	from	presence	1432:1439	arg1	samples					1508:1514	pregnancy hCG samples	1494:1514	pregnancy hCG samples	1494:1514	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	13	23	theme	decreased	1809:1817	arg1	signals					1819:1825	decreased signals	1809:1825	decreased signals for tri- and tetra-antennary N-glycans	1809:1864	When compared to EP-hCG, GTD-hCG samples had decreased signals for tri- and tetra-antennary N-glycans.					
32045434	0	24	theme	glycomics	81:89	arg1	analysis					91:98	glycomics analysis	81:98	glycomics analysis	81:98	Insights into the hyperglycosylation of human chorionic gonadotropin revealed by glycomics analysis.					
32045434	11	25	from	presence	1608:1615	arg1	N-glycans					1661:1669	the bisected N-glycans	1648:1669	the bisected N-glycans	1648:1669	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	17	26	theme	potential	2190:2198	arg1	structures					2175:2184	Bisected and LewisX structures	2155:2184	Bisected and LewisX structures	2155:2184	Bisected and LewisX structures are potential targets for diagnostics.					
32045434	17	26	theme	potential	2190:2198	arg1	targets					2200:2206	potential targets	2190:2206	potential targets for diagnostics	2190:2222	Bisected and LewisX structures are potential targets for diagnostics.					
32045434	18	27	theme	bisected	2448:2455	arg1	N-glycans					2462:2470	hCG-related bisected type N-glycans	2436:2470	hCG-related bisected type N-glycans	2436:2470	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	5	28	theme	comparative	587:597	arg1	analysis					610:617	comparative N-glycomic analysis	587:617	comparative N-glycomic analysis of hCG expressed in the same individuals	587:658	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	18	29	theme	NK	2494:2495	arg1	cytotoxicity					2502:2513	NK cell cytotoxicity	2494:2513	NK cell cytotoxicity	2494:2513	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	5	30	theme	hCG	726:728	arg1	function					730:737	hCG function	726:737	hCG function	726:737	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	14	31	theme	sialylated	1932:1941	arg1	structures					1943:1952	sialylated structures	1932:1952	sialylated structures	1932:1952	In terms of terminal epitopes, GTD-hCG had increased signals for sialylated structures, while LewisX antigens were of very minor abundance.					
32045434	13	32	contain	had	1805:1807	arg2	signals					1819:1825	decreased signals	1809:1825	decreased signals for tri- and tetra-antennary N-glycans	1809:1864	When compared to EP-hCG, GTD-hCG samples had decreased signals for tri- and tetra-antennary N-glycans.					
32045434	13	32	contain	had	1805:1807	arg1	samples					1797:1803	GTD-hCG samples	1789:1803	GTD-hCG samples	1789:1803	When compared to EP-hCG, GTD-hCG samples had decreased signals for tri- and tetra-antennary N-glycans.					
32045434	3	33	theme	embryo	415:420	arg1	implantation					395:406	initial implantation	387:406	initial implantation of the embryo	387:420	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	1	34	theme	Human	101:105	arg1	essential					170:178	essential	170:178	essential	170:178	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	1	34	theme	Human	101:105	arg1	hormone					154:160	a glycoprotein hormone	139:160	a glycoprotein hormone that is essential for the maintenance of pregnancy	139:211	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	1	34	theme	Human	101:105	arg1	hCG					131:133	hCG	131:133	hCG	131:133	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	1	34	theme	Human	101:105	arg1	gonadotropin					117:128	Human chorionic gonadotropin	101:128	Human chorionic gonadotropin (hCG)	101:134	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	18	35	theme	cell	2401:2404	arg1	cytotoxicity					2406:2417	NK cell cytotoxicity	2398:2417	NK cell cytotoxicity	2398:2417	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	17	36	theme	Bisected	2155:2162	arg1	structures					2175:2184	Bisected and LewisX structures	2155:2184	Bisected and LewisX structures	2155:2184	Bisected and LewisX structures are potential targets for diagnostics.					
32045434	17	36	theme	Bisected	2155:2162	arg1	targets					2200:2206	potential targets	2190:2206	potential targets for diagnostics	2190:2222	Bisected and LewisX structures are potential targets for diagnostics.					
32045434	18	37	theme	NK	2268:2269	arg1	cytotoxicity					2276:2287	NK cell cytotoxicity	2268:2287	NK cell cytotoxicity	2268:2287	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	14	38	theme	minor	1990:1994	arg1	abundance					1996:2004	very minor abundance	1985:2004	very minor abundance	1985:2004	In terms of terminal epitopes, GTD-hCG had increased signals for sialylated structures, while LewisX antigens were of very minor abundance.					
32045434	11	39	theme	LewisX	1629:1634	arg1	antigens					1636:1643	abundant LewisX antigens	1620:1643	abundant LewisX antigens	1620:1643	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	3	40	theme	potential	431:439	arg1	variants					309:316	"Hyperglycosylated" variants	289:316	"Hyperglycosylated" variants secreted during early pregnancy	289:348	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	3	40	theme	potential	431:439	arg1	marker					452:457	a potential diagnostic marker	429:457	a potential diagnostic marker for gestational diseases	429:482	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	8	41	theme	matrix	1049:1054	arg1	MS					1139:1140	MS	1139:1140	MS	1139:1140	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	41	theme	matrix	1049:1054	arg1	spectrometry					1125:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry	1049:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1049:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	3	42	theme	initial	387:393	arg1	implantation					395:406	initial implantation	387:406	initial implantation of the embryo	387:420	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	4	43	theme	hyperglycosylation	512:529	arg1	"					530:530	"hyperglycosylation"	511:530	"hyperglycosylation"	511:530	However, what constitutes "hyperglycosylation" is not yet fully understood.					
32045434	10	44	theme	bisected	1467:1474	arg1	N-glycans					1481:1489	bisected type N-glycans	1467:1489	bisected type N-glycans	1467:1489	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	0	45	theme	chorionic	46:54	arg1	gonadotropin					56:67	human chorionic gonadotropin	40:67	human chorionic gonadotropin	40:67	Insights into the hyperglycosylation of human chorionic gonadotropin revealed by glycomics analysis.					
32045434	8	46	theme	laser	1065:1069	arg1	MS					1139:1140	MS	1139:1140	MS	1139:1140	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	46	theme	laser	1065:1069	arg1	spectrometry					1125:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry	1049:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1049:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	0	47	gly	hyperglycosylation	18:35	arg1	gonadotropin					56:67	human chorionic gonadotropin	40:67	human chorionic gonadotropin	40:67	Insights into the hyperglycosylation of human chorionic gonadotropin revealed by glycomics analysis.					
32045434	10	48	theme	amounts	1456:1462	arg1	presence					1432:1439	the presence	1428:1439	the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples	1428:1514	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	10	48	theme	amounts	1456:1462	arg1	finding					1416:1422	A novel finding	1408:1422	A novel finding	1408:1422	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	11	49	theme	bisected	1652:1659	arg1	N-glycans					1661:1669	the bisected N-glycans	1648:1669	the bisected N-glycans	1648:1669	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	3	50	theme	gestational	463:473	arg1	diseases					475:482	gestational diseases	463:482	gestational diseases	463:482	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	8	51	theme	of	1098:1099	arg1	MS					1139:1140	MS	1139:1140	MS	1139:1140	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	51	theme	of	1098:1099	arg1	spectrometry					1125:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry	1049:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1049:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	15	52	theme	same	2023:2026	arg1	N-glycans					2028:2036	the same N-glycans	2019:2036	the same N-glycans throughout pregnancy but in different proportions	2019:2086	hCG carries the same N-glycans throughout pregnancy but in different proportions.					
32045434	10	53	theme	pregnancy	1494:1502	arg1	samples					1508:1514	pregnancy hCG samples	1494:1514	pregnancy hCG samples	1494:1514	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	3	54	theme	diagnostic	441:450	arg1	variants					309:316	"Hyperglycosylated" variants	289:316	"Hyperglycosylated" variants secreted during early pregnancy	289:348	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	3	54	theme	diagnostic	441:450	arg1	marker					452:457	a potential diagnostic marker	429:457	a potential diagnostic marker for gestational diseases	429:482	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	10	55	attach	presence	1432:1439	arg1	samples					1508:1514	pregnancy hCG samples	1494:1514	pregnancy hCG samples	1494:1514	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	10	55	attach	presence	1432:1439	arg2	amounts					1456:1462	substantial amounts	1444:1462	substantial amounts of bisected type N-glycans	1444:1489	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	10	55	attach	presence	1432:1439	arg2	N-glycans					1481:1489	bisected type N-glycans	1467:1489	bisected type N-glycans	1467:1489	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	3	56	gly	Hyperglycosylated	290:306	arg1	variants					309:316	"Hyperglycosylated" variants	289:316	"Hyperglycosylated" variants secreted during early pregnancy	289:348	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	3	56	gly	Hyperglycosylated	290:306	arg1	marker					452:457	a potential diagnostic marker	429:457	a potential diagnostic marker for gestational diseases	429:482	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	12	57	theme	those	1731:1735	arg1	subset					1721:1726	a subset	1719:1726	a subset of those found in normal pregnancy	1719:1761	GTD-hCG had fewer glycoforms which constituted a subset of those found in normal pregnancy.					
32045434	10	58	theme	N-glycans	1481:1489	arg1	amounts					1456:1462	substantial amounts	1444:1462	substantial amounts of bisected type N-glycans	1444:1489	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	10	58	theme	N-glycans	1481:1489	arg1	N-glycans					1481:1489	bisected type N-glycans	1467:1489	bisected type N-glycans	1467:1489	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	11	59	theme	second	1575:1580	arg1	observation					1588:1598	A second novel observation	1573:1598	A second novel observation	1573:1598	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	11	59	theme	second	1575:1580	arg1	presence					1608:1615	the presence	1604:1615	the presence of abundant LewisX antigens on the bisected N-glycans	1604:1669	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	5	60	theme	same	643:646	arg1	individuals					648:658	the same individuals	639:658	the same individuals	639:658	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	10	61	from	levels	1550:1555	arg1	samples					1564:1570	GTD samples	1560:1570	GTD samples	1560:1570	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	10	61	from	levels	1550:1555	arg1	present					1528:1534	present	1528:1534	present	1528:1534	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	5	62	gly	hyperglycosylated	803:819	arg1	hCG					821:823	hyperglycosylated hCG	803:823	hyperglycosylated hCG	803:823	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	18	63	theme	type	2331:2334	arg1	glycans					2336:2342	bisected type glycans	2322:2342	bisected type glycans	2322:2342	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	5	64	theme	new	747:749	arg1	targets					751:757	new targets	747:757	new targets for diagnostics	747:773	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	5	65	theme	hCG	622:624	arg1	analysis					610:617	comparative N-glycomic analysis	587:617	comparative N-glycomic analysis of hCG expressed in the same individuals	587:658	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	9	66	theme	tetra-antennary	1381:1395	arg1	N-glycans					1397:1405	tetra-antennary N-glycans	1381:1405	tetra-antennary N-glycans	1381:1405	The structures identified on the early pregnancy (EP-hCG) and late pregnancy (LP-hCG) samples corresponded to mono-, bi-, tri-, and tetra-antennary N-glycans.					
32045434	15	67	from	N-glycans	2028:2036	arg1	proportions					2076:2086	different proportions	2066:2086	different proportions	2066:2086	hCG carries the same N-glycans throughout pregnancy but in different proportions.					
32045434	7	68	from	women	945:949	arg1	urine					934:938	urine	934:938	urine from women diagnosed with gestational trophoblastic disease (GTD)	934:1004	hCG was also isolated in urine from women diagnosed with gestational trophoblastic disease (GTD).					
32045434	14	69	theme	terminal	1879:1886	arg1	epitopes					1888:1895	terminal epitopes	1879:1895	terminal epitopes	1879:1895	In terms of terminal epitopes, GTD-hCG had increased signals for sialylated structures, while LewisX antigens were of very minor abundance.					
32045434	5	70	theme	hyperglycosylated	803:819	arg1	hCG					821:823	hyperglycosylated hCG	803:823	hyperglycosylated hCG	803:823	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	10	71	theme	GTD	1560:1562	arg1	samples					1564:1570	GTD samples	1560:1570	GTD samples	1560:1570	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	11	72	attach	presence	1608:1615	arg1	N-glycans					1661:1669	the bisected N-glycans	1648:1669	the bisected N-glycans	1648:1669	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	11	72	attach	presence	1608:1615	arg2	antigens					1636:1643	abundant LewisX antigens	1620:1643	abundant LewisX antigens	1620:1643	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	1	73	theme	pregnancy	203:211	arg1	maintenance					188:198	the maintenance	184:198	the maintenance of pregnancy	184:211	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	2	74	gly	Glycosylation	214:226	arg1	hCG					231:233	hCG	231:233	hCG	231:233	Glycosylation of hCG is known to be essential for its biological activity.					
32045434	8	75	used	used	1010:1013	arg2	We					1007:1008	We	1007:1008	We	1007:1008	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	1	76	gly	glycoprotein	141:152	arg1	glycoprotein					141:152	a glycoprotein hormone	139:160	a glycoprotein hormone that is essential for the maintenance of pregnancy	139:211	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	7	77	theme	trophoblastic	978:990	arg1	GTD					1001:1003	GTD	1001:1003	GTD	1001:1003	hCG was also isolated in urine from women diagnosed with gestational trophoblastic disease (GTD).					
32045434	7	77	theme	trophoblastic	978:990	arg1	disease					992:998	gestational trophoblastic disease	966:998	gestational trophoblastic disease (GTD)	966:1004	hCG was also isolated in urine from women diagnosed with gestational trophoblastic disease (GTD).					
32045434	3	78	theme	"	307:307	arg1	variants					309:316	"Hyperglycosylated" variants	289:316	"Hyperglycosylated" variants secreted during early pregnancy	289:348	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	3	78	theme	"	307:307	arg1	marker					452:457	a potential diagnostic marker	429:457	a potential diagnostic marker for gestational diseases	429:482	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	18	79	theme	cytotoxicity	2406:2417	arg1	suppression					2383:2393	the suppression	2379:2393	the suppression of NK cell cytotoxicity	2379:2417	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	13	80	theme	tri-	1831:1834	arg1	N-glycans					1856:1864	tri- and tetra-antennary N-glycans	1831:1864	tri- and tetra-antennary N-glycans	1831:1864	When compared to EP-hCG, GTD-hCG samples had decreased signals for tri- and tetra-antennary N-glycans.					
32045434	14	81	gly	sialylated	1932:1941	arg1	structures					1943:1952	sialylated structures	1932:1952	sialylated structures	1932:1952	In terms of terminal epitopes, GTD-hCG had increased signals for sialylated structures, while LewisX antigens were of very minor abundance.					
32045434	15	82	contain	carries	2011:2017	arg1	hCG					2007:2009	hCG	2007:2009	hCG	2007:2009	hCG carries the same N-glycans throughout pregnancy but in different proportions.					
32045434	15	82	contain	carries	2011:2017	arg2	N-glycans					2028:2036	the same N-glycans	2019:2036	the same N-glycans throughout pregnancy but in different proportions	2019:2086	hCG carries the same N-glycans throughout pregnancy but in different proportions.					
32045434	13	83	theme	tetra-antennary	1840:1854	arg1	N-glycans					1856:1864	tri- and tetra-antennary N-glycans	1831:1864	tri- and tetra-antennary N-glycans	1831:1864	When compared to EP-hCG, GTD-hCG samples had decreased signals for tri- and tetra-antennary N-glycans.					
32045434	12	84	contain	had	1680:1682	arg1	GTD-hCG					1672:1678	GTD-hCG	1672:1678	GTD-hCG	1672:1678	GTD-hCG had fewer glycoforms which constituted a subset of those found in normal pregnancy.					
32045434	12	84	contain	had	1680:1682	arg2	glycoforms					1690:1699	fewer glycoforms	1684:1699	fewer glycoforms which constituted a subset of those found in normal pregnancy	1684:1761	GTD-hCG had fewer glycoforms which constituted a subset of those found in normal pregnancy.					
32045434	3	85	theme	early	334:338	arg1	pregnancy					340:348	early pregnancy	334:348	early pregnancy	334:348	"Hyperglycosylated" variants secreted during early pregnancy have been proposed to be involved in initial implantation of the embryo and as a potential diagnostic marker for gestational diseases.					
32045434	17	86	theme	LewisX	2168:2173	arg1	structures					2175:2184	Bisected and LewisX structures	2155:2184	Bisected and LewisX structures	2155:2184	Bisected and LewisX structures are potential targets for diagnostics.					
32045434	17	86	theme	LewisX	2168:2173	arg1	targets					2200:2206	potential targets	2190:2206	potential targets for diagnostics	2190:2222	Bisected and LewisX structures are potential targets for diagnostics.					
32045434	18	87	theme	nanomolar	2301:2309	arg1	levels					2311:2316	nanomolar levels	2301:2316	nanomolar levels	2301:2316	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	14	88	theme	LewisX	1961:1966	arg1	antigens					1968:1975	LewisX antigens	1961:1975	LewisX antigens	1961:1975	In terms of terminal epitopes, GTD-hCG had increased signals for sialylated structures, while LewisX antigens were of very minor abundance.					
32045434	18	89	theme	type	2457:2460	arg1	N-glycans					2462:2470	hCG-related bisected type N-glycans	2436:2470	hCG-related bisected type N-glycans	2436:2470	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	5	90	theme	new	708:710	arg1	insights					712:719	new insights	708:719	new insights into hCG function	708:737	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	18	91	theme	hCG-related	2436:2446	arg1	N-glycans					2462:2470	hCG-related bisected type N-glycans	2436:2470	hCG-related bisected type N-glycans	2436:2470	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	9	92	theme	early	1282:1286	arg1	EP-hCG					1299:1304	EP-hCG	1299:1304	EP-hCG	1299:1304	The structures identified on the early pregnancy (EP-hCG) and late pregnancy (LP-hCG) samples corresponded to mono-, bi-, tri-, and tetra-antennary N-glycans.					
32045434	9	92	theme	early	1282:1286	arg1	pregnancy					1288:1296	the early pregnancy	1278:1296	the early pregnancy (EP-hCG)	1278:1305	The structures identified on the early pregnancy (EP-hCG) and late pregnancy (LP-hCG) samples corresponded to mono-, bi-, tri-, and tetra-antennary N-glycans.					
32045434	5	93	theme	N-glycomic	599:608	arg1	analysis					610:617	comparative N-glycomic analysis	587:617	comparative N-glycomic analysis of hCG expressed in the same individuals	587:658	In this study, we perform comparative N-glycomic analysis of hCG expressed in the same individuals during early and late pregnancy to help provide new insights into hCG function, reveal new targets for diagnostics and clarify the identity of hyperglycosylated hCG.					
32045434	18	94	theme	pregnancy	2243:2251	arg1	urine					2253:2257	pregnancy urine	2243:2257	pregnancy urine	2243:2257	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	10	95	theme	novel	1410:1414	arg1	presence					1432:1439	the presence	1428:1439	the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples	1428:1514	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	10	95	theme	novel	1410:1414	arg1	finding					1416:1422	A novel finding	1408:1422	A novel finding	1408:1422	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	13	96	theme	GTD-hCG	1789:1795	arg1	samples					1797:1803	GTD-hCG samples	1789:1803	GTD-hCG samples	1789:1803	When compared to EP-hCG, GTD-hCG samples had decreased signals for tri- and tetra-antennary N-glycans.					
32045434	8	97	theme	glycomics	1015:1023	arg1	methodologies					1025:1037	glycomics methodologies	1015:1037	glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1015:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	97	theme	glycomics	1015:1023	arg1	spectrometry					1125:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry	1049:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1049:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	12	98	located	found	1737:1741	arg2	those					1731:1735	those	1731:1735	those	1731:1735	GTD-hCG had fewer glycoforms which constituted a subset of those found in normal pregnancy.					
32045434	12	98	located	found	1737:1741	arg1	pregnancy					1753:1761	normal pregnancy	1746:1761	normal pregnancy	1746:1761	GTD-hCG had fewer glycoforms which constituted a subset of those found in normal pregnancy.					
32045434	18	99	attach	isolated	2229:2236	arg2	hCG					2225:2227	hCG	2225:2227	hCG isolated from pregnancy urine	2225:2257	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	18	99	attach	isolated	2229:2236	arg1	urine					2253:2257	pregnancy urine	2243:2257	pregnancy urine	2243:2257	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	1	100	theme	chorionic	107:115	arg1	essential					170:178	essential	170:178	essential	170:178	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	1	100	theme	chorionic	107:115	arg1	hormone					154:160	a glycoprotein hormone	139:160	a glycoprotein hormone that is essential for the maintenance of pregnancy	139:211	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	1	100	theme	chorionic	107:115	arg1	hCG					131:133	hCG	131:133	hCG	131:133	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	1	100	theme	chorionic	107:115	arg1	gonadotropin					117:128	Human chorionic gonadotropin	101:128	Human chorionic gonadotropin (hCG)	101:134	Human chorionic gonadotropin (hCG) is a glycoprotein hormone that is essential for the maintenance of pregnancy.					
32045434	18	101	theme	cell	2271:2274	arg1	cytotoxicity					2276:2287	NK cell cytotoxicity	2268:2287	NK cell cytotoxicity	2268:2287	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	18	102	theme	NK	2398:2399	arg1	cytotoxicity					2406:2417	NK cell cytotoxicity	2398:2417	NK cell cytotoxicity	2398:2417	hCG isolated from pregnancy urine inhibits NK cell cytotoxicity in vitro at nanomolar levels and bisected type glycans have previously been implicated in the suppression of NK cell cytotoxicity, suggesting that hCG-related bisected type N-glycans may directly suppress NK cell cytotoxicity.					
32045434	11	103	theme	abundant	1620:1627	arg1	antigens					1636:1643	abundant LewisX antigens	1620:1643	abundant LewisX antigens	1620:1643	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	0	104	theme	human	40:44	arg1	gonadotropin					56:67	human chorionic gonadotropin	40:67	human chorionic gonadotropin	40:67	Insights into the hyperglycosylation of human chorionic gonadotropin revealed by glycomics analysis.					
32045434	9	105	theme	late	1311:1314	arg1	LP-hCG					1327:1332	LP-hCG	1327:1332	LP-hCG	1327:1332	The structures identified on the early pregnancy (EP-hCG) and late pregnancy (LP-hCG) samples corresponded to mono-, bi-, tri-, and tetra-antennary N-glycans.					
32045434	9	105	theme	late	1311:1314	arg1	pregnancy					1316:1324	late pregnancy	1311:1324	late pregnancy (LP-hCG)	1311:1333	The structures identified on the early pregnancy (EP-hCG) and late pregnancy (LP-hCG) samples corresponded to mono-, bi-, tri-, and tetra-antennary N-glycans.					
32045434	8	106	theme	assisted	1056:1063	arg1	MS					1139:1140	MS	1139:1140	MS	1139:1140	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	106	theme	assisted	1056:1063	arg1	spectrometry					1125:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry	1049:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1049:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	9	107	dep	pregnancy	1288:1296	arg1	samples					1335:1341	samples	1335:1341	samples	1335:1341	The structures identified on the early pregnancy (EP-hCG) and late pregnancy (LP-hCG) samples corresponded to mono-, bi-, tri-, and tetra-antennary N-glycans.					
32045434	10	108	dep	presence	1432:1439	arg1	present					1528:1534	present	1528:1534	present	1528:1534	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	0	109	theme	gonadotropin	56:67	arg1	hyperglycosylation					18:35	the hyperglycosylation	14:35	the hyperglycosylation of human chorionic gonadotropin	14:67	Insights into the hyperglycosylation of human chorionic gonadotropin revealed by glycomics analysis.					
32045434	8	110	theme	desorption/ionisation-time	1071:1096	arg1	MS					1139:1140	MS	1139:1140	MS	1139:1140	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	110	theme	desorption/ionisation-time	1071:1096	arg1	spectrometry					1125:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry	1049:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1049:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	14	111	theme	epitopes	1888:1895	arg1	terms					1870:1874	terms	1870:1874	terms of terminal epitopes	1870:1895	In terms of terminal epitopes, GTD-hCG had increased signals for sialylated structures, while LewisX antigens were of very minor abundance.					
32045434	8	112	theme	flight	1101:1106	arg1	MS					1139:1140	MS	1139:1140	MS	1139:1140	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	112	theme	flight	1101:1106	arg1	spectrometry					1125:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry	1049:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1049:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	12	113	theme	normal	1746:1751	arg1	pregnancy					1753:1761	normal pregnancy	1746:1761	normal pregnancy	1746:1761	GTD-hCG had fewer glycoforms which constituted a subset of those found in normal pregnancy.					
32045434	10	114	theme	substantial	1444:1454	arg1	amounts					1456:1462	substantial amounts	1444:1462	substantial amounts of bisected type N-glycans	1444:1489	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	10	114	theme	substantial	1444:1454	arg1	N-glycans					1481:1489	bisected type N-glycans	1467:1489	bisected type N-glycans	1467:1489	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	11	115	theme	antigens	1636:1643	arg1	observation					1588:1598	A second novel observation	1573:1598	A second novel observation	1573:1598	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	11	115	theme	antigens	1636:1643	arg1	presence					1608:1615	the presence	1604:1615	the presence of abundant LewisX antigens on the bisected N-glycans	1604:1669	A second novel observation was the presence of abundant LewisX antigens on the bisected N-glycans.					
32045434	16	116	theme	N-glycan	2093:2100	arg1	repertoire					2102:2111	The N-glycan repertoire	2089:2111	The N-glycan repertoire	2089:2111	The N-glycan repertoire is more diverse than previously reported.					
32045434	16	116	theme	N-glycan	2093:2100	arg1	diverse					2121:2127	diverse	2121:2127	diverse	2121:2127	The N-glycan repertoire is more diverse than previously reported.					
32045434	8	117	theme	MALDI-TOF	1109:1117	arg1	MS					1139:1140	MS	1139:1140	MS	1139:1140	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	8	117	theme	MALDI-TOF	1109:1117	arg1	spectrometry					1125:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry	1049:1136	matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS)	1049:1141	We used glycomics methodologies including matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and MS/MS methods to characterise the N-glycans associated with hCG purified from the individual samples.					
32045434	10	118	located	present	1528:1534	arg2	which					1517:1521	which	1517:1521	which	1517:1521	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
32045434	10	118	located	present	1528:1534	arg1	levels					1550:1555	much lower levels	1539:1555	much lower levels in GTD samples	1539:1570	A novel finding was the presence of substantial amounts of bisected type N-glycans in pregnancy hCG samples, which were present at much lower levels in GTD samples.					
33661628	1	0	theme	units	364:368	arg1	arrangements					319:330	branched arrangements	310:330	branched arrangements of mannose (Man) monosaccharide units	310:368	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	1	1	theme	-[Manα	224:229	arg1	-GlcNAcβ1-Asn					259:271	Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn	215:271	Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn	215:271	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	8	2	from	forms	1821:1825	arg1	solution					1830:1837	solution	1830:1837	solution	1830:1837	More importantly, we find that the protein does not actively distort the N-glycans into structures not populated in the unlinked forms in solution.					
33661628	6	3	theme	protein	1155:1161	arg1	surface					1163:1169	a protein surface	1153:1169	a protein surface	1153:1169	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	4	theme	antibody-dependent	1402:1419	arg1	ADCC					1444:1447	ADCC	1444:1447	ADCC	1444:1447	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	4	theme	antibody-dependent	1402:1419	arg1	cytotoxicity					1430:1441	the antibody-dependent cellular cytotoxicity	1398:1441	the antibody-dependent cellular cytotoxicity (ADCC)	1398:1448	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	7	5	theme	surface	1673:1679	arg1	landscape					1681:1689	the protein's surface landscape	1659:1689	the protein's surface landscape	1659:1689	Our results show that the protein's structural constraints shift the oligomannoses conformational ensemble to promote conformers that satisfy the steric requirements and hydrogen bonding networks demanded by the protein's surface landscape.					
33661628	6	6	from	effects	1142:1148	arg1	equilibria					1191:1200	their structural equilibria	1174:1200	their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC)	1174:1448	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	0	7	theme	FcγRIIIa	63:70	arg1	Landscape					83:91	the FcγRIIIa Structural Landscape	59:91	the FcγRIIIa Structural Landscape	59:91	Oligomannose N-Glycans 3D Architecture and Its Response to the FcγRIIIa Structural Landscape.					
33661628	3	8	from	roles	626:630	arg1	escape					642:647	immune escape	635:647	immune escape	635:647	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	9	9	from	N162	2038:2041	arg1	presentation					2010:2021	a specific presentation	1999:2021	a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc	1999:2109	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	9	9	from	N162	2038:2041	arg1	arms					2030:2033	the arms	2026:2033	the arms	2026:2033	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	1	10	theme	common	180:185	arg1	unit					203:206	the common pentasaccharide unit	176:206	the common pentasaccharide unit	176:206	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	6	11	theme	μs	1218:1219	arg1	MD					1232:1233	over 4 μs cumulative MD	1211:1233	over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC)	1211:1448	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	1	12	theme	branched	310:317	arg1	arrangements					319:330	branched arrangements	310:330	branched arrangements of mannose (Man) monosaccharide units	310:368	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	1	13	theme	unit	203:206	arg1	unit					203:206	the common pentasaccharide unit	176:206	the common pentasaccharide unit	176:206	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	1	13	theme	unit	203:206	arg1	arms					168:171	the arms	164:171	the arms	164:171	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	7	14	theme	structural	1487:1496	arg1	constraints					1498:1508	the protein's structural constraints	1473:1508	the protein's structural constraints	1473:1508	Our results show that the protein's structural constraints shift the oligomannoses conformational ensemble to promote conformers that satisfy the steric requirements and hydrogen bonding networks demanded by the protein's surface landscape.					
33661628	5	15	theme	common	1099:1104	arg1	N-glycans					1089:1097	differently processed, free (not protein-linked) oligomannose N-glycans	1027:1097	differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates	1027:1119	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	5	16	theme	sampling	976:983	arg1	μs					959:960	over 54 μs	951:960	over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates	951:1119	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	2	17	theme	yeast	488:492	arg1	mannan					494:499	yeast mannan	488:499	yeast mannan	488:499	In mammalian species oligomannose N-glycans can have up to 9 Man; meanwhile structures can grow to over 200 units in yeast mannan.					
33661628	3	18	theme	enveloped	668:676	arg1	viruses					678:684	enveloped viruses	668:684	enveloped viruses	668:684	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	3	18	theme	enveloped	668:676	arg1	SARS-CoV2					705:713	SARS-CoV2	705:713	SARS-CoV2	705:713	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	3	18	theme	enveloped	668:676	arg1	HIV-1					695:699	HIV-1	695:699	HIV-1	695:699	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	8	19	theme	unlinked	1812:1819	arg1	forms					1821:1825	the unlinked forms	1808:1825	the unlinked forms in solution	1808:1837	More importantly, we find that the protein does not actively distort the N-glycans into structures not populated in the unlinked forms in solution.					
33661628	1	20	theme	oldest	131:136	arg1	class					138:142	the oldest class	127:142	the oldest class of N-glycans	127:155	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	1	20	theme	oldest	131:136	arg1	Oligomannoses					94:106	Oligomannoses	94:106	Oligomannoses	94:106	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	7	21	theme	bonding	1630:1636	arg1	networks					1638:1645	hydrogen bonding networks	1621:1645	hydrogen bonding networks	1621:1645	Our results show that the protein's structural constraints shift the oligomannoses conformational ensemble to promote conformers that satisfy the steric requirements and hydrogen bonding networks demanded by the protein's surface landscape.					
33661628	6	22	theme	structural	1180:1189	arg1	equilibria					1191:1200	their structural equilibria	1174:1200	their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC)	1174:1448	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	3	23	theme	branching	529:537	arg1	complexity					539:548	branching complexity	529:548	branching complexity	529:548	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	4	24	theme	structural	878:887	arg1	characterization					889:904	their structural characterization	872:904	their structural characterization	872:904	The architectural features that allow these N-glycans to perform their functions are yet unclear, due to their intrinsically disordered nature that hinders their structural characterization.					
33661628	9	25	theme	populated	1863:1871	arg1	conformations					1873:1885	the highly populated conformations	1852:1885	the highly populated conformations of the Man5 linked glycans	1852:1912	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	9	26	from	N45	1986:1988	arg1	forms					1977:1981	hybrid complex forms	1962:1981	hybrid complex forms at N45	1962:1988	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	9	26	from	N45	1986:1988	arg1	levels					1952:1957	high levels	1947:1957	high levels of hybrid complex forms at N45	1947:1988	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	7	27	theme	steric	1597:1602	arg1	requirements					1604:1615	the steric requirements	1593:1615	the steric requirements	1593:1615	Our results show that the protein's structural constraints shift the oligomannoses conformational ensemble to promote conformers that satisfy the steric requirements and hydrogen bonding networks demanded by the protein's surface landscape.					
33661628	0	28	theme	Structural	72:81	arg1	Landscape					83:91	the FcγRIIIa Structural Landscape	59:91	the FcγRIIIa Structural Landscape	59:91	Oligomannose N-Glycans 3D Architecture and Its Response to the FcγRIIIa Structural Landscape.					
33661628	5	29	theme	molecular	988:996	arg1	MD					1008:1009	MD	1008:1009	MD	1008:1009	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	5	29	theme	molecular	988:996	arg1	dynamics					998:1005	molecular dynamics	988:1005	molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates	988:1119	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	9	30	theme	glycans	1906:1912	arg1	conformations					1873:1885	the highly populated conformations	1852:1885	the highly populated conformations of the Man5 linked glycans	1852:1912	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	9	31	from	levels	1952:1957	arg1	N45					1986:1988	N45	1986:1988	N45	1986:1988	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	2	32	theme	mammalian	374:382	arg1	species					384:390	mammalian species	374:390	mammalian species	374:390	In mammalian species oligomannose N-glycans can have up to 9 Man; meanwhile structures can grow to over 200 units in yeast mannan.					
33661628	3	33	from	infectivity	653:663	arg1	escape					642:647	immune escape	635:647	immune escape	635:647	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	9	34	theme	experimental	1922:1933	arg1	evidence					1935:1942	experimental evidence	1922:1942	experimental evidence of high levels of hybrid complex forms at N45	1922:1988	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	1	35	theme	monosaccharide	349:362	arg1	units					364:368	mannose (Man) monosaccharide units	335:368	mannose (Man) monosaccharide units	335:368	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	7	36	theme	conformational	1534:1547	arg1	ensemble					1549:1556	the oligomannoses conformational ensemble	1516:1556	the oligomannoses conformational ensemble to promote conformers that satisfy the steric requirements and hydrogen bonding networks demanded by the protein's surface landscape	1516:1689	Our results show that the protein's structural constraints shift the oligomannoses conformational ensemble to promote conformers that satisfy the steric requirements and hydrogen bonding networks demanded by the protein's surface landscape.					
33661628	3	37	theme	3D	555:556	arg1	structure					558:566	3D structure	555:566	3D structure	555:566	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	0	38	theme	3D	23:24	arg1	Architecture					26:37	3D Architecture	23:37	3D Architecture	23:37	Oligomannose N-Glycans 3D Architecture and Its Response to the FcγRIIIa Structural Landscape.					
33661628	1	39	theme	-Manβ	236:240	arg1	-GlcNAcβ1-Asn					259:271	Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn	215:271	Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn	215:271	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	7	40	theme	oligomannoses	1520:1532	arg1	ensemble					1549:1556	the oligomannoses conformational ensemble	1516:1556	the oligomannoses conformational ensemble to promote conformers that satisfy the steric requirements and hydrogen bonding networks demanded by the protein's surface landscape	1516:1689	Our results show that the protein's structural constraints shift the oligomannoses conformational ensemble to promote conformers that satisfy the steric requirements and hydrogen bonding networks demanded by the protein's surface landscape.					
33661628	9	41	theme	levels	1952:1957	arg1	evidence					1935:1942	experimental evidence	1922:1942	experimental evidence of high levels of hybrid complex forms at N45	1922:1988	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	5	42	theme	processed	1039:1047	arg1	N-glycans					1089:1097	differently processed, free (not protein-linked) oligomannose N-glycans	1027:1097	differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates	1027:1119	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	2	43	theme	oligomannose	392:403	arg1	N-glycans					405:413	oligomannose N-glycans	392:413	oligomannose N-glycans	392:413	In mammalian species oligomannose N-glycans can have up to 9 Man; meanwhile structures can grow to over 200 units in yeast mannan.					
33661628	9	44	theme	hybrid	1962:1967	arg1	forms					1977:1981	hybrid complex forms	1962:1981	hybrid complex forms at N45	1962:1988	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	6	45	theme	γ	1279:1279	arg1	FcγRIIIa					1291:1298	FcγRIIIa	1291:1298	FcγRIIIa	1291:1298	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	45	theme	γ	1279:1279	arg1	receptor					1281:1288	the fully glycosylated CD16a Fc γ receptor	1247:1288	the fully glycosylated CD16a Fc γ receptor (FcγRIIIa)	1247:1299	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	1	46	theme	-GlcNAcβ	246:253	arg1	-GlcNAcβ1-Asn					259:271	Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn	215:271	Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn	215:271	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	9	47	theme	forms	1977:1981	arg1	levels					1952:1957	high levels	1947:1957	high levels of hybrid complex forms at N45	1947:1988	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	6	48	gly	glycosylated	1257:1268	arg1	FcγRIIIa					1291:1298	FcγRIIIa	1291:1298	FcγRIIIa	1291:1298	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	48	gly	glycosylated	1257:1268	arg1	receptor					1281:1288	the fully glycosylated CD16a Fc γ receptor	1247:1288	the fully glycosylated CD16a Fc γ receptor (FcγRIIIa)	1247:1299	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	49	theme	glycosylation	1320:1332	arg1	type					1312:1315	the type	1308:1315	the type of glycosylation	1308:1332	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	5	50	theme	N-glycans	1089:1097	arg1	simulations					1012:1022	molecular dynamics (MD) simulations	988:1022	molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates	988:1119	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	9	51	theme	specific	2001:2008	arg1	presentation					2010:2021	a specific presentation	1999:2021	a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc	1999:2109	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	6	52	theme	surface	1163:1169	arg1	effects					1142:1148	the effects	1138:1148	the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC)	1138:1448	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	3	53	theme	immune	635:640	arg1	escape					642:647	immune escape	635:647	immune escape	635:647	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	9	54	theme	arms	2030:2033	arg1	presentation					2010:2021	a specific presentation	1999:2021	a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc	1999:2109	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	3	55	theme	oligomannoses	571:583	arg1	nature					521:526	The highly dynamic nature	502:526	The highly dynamic nature	502:526	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	3	55	theme	oligomannoses	571:583	arg1	complexity					539:548	branching complexity	529:548	branching complexity	529:548	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	3	55	theme	oligomannoses	571:583	arg1	structure					558:566	3D structure	555:566	3D structure	555:566	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	6	56	theme	cumulative	1221:1230	arg1	MD					1232:1233	over 4 μs cumulative MD	1211:1233	over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC)	1211:1448	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	1	57	theme	pentasaccharide	187:201	arg1	unit					203:206	the common pentasaccharide unit	176:206	the common pentasaccharide unit	176:206	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	5	58	theme	cumulative	965:974	arg1	sampling					976:983	cumulative sampling	965:983	cumulative sampling	965:983	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	6	59	theme	binding	1359:1365	arg1	affinity					1367:1374	its binding affinity	1355:1374	its binding affinity for IgG1s	1355:1384	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	60	theme	CD16a	1270:1274	arg1	FcγRIIIa					1291:1298	FcγRIIIa	1291:1298	FcγRIIIa	1291:1298	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	60	theme	CD16a	1270:1274	arg1	receptor					1281:1288	the fully glycosylated CD16a Fc γ receptor	1247:1288	the fully glycosylated CD16a Fc γ receptor (FcγRIIIa)	1247:1299	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	1	61	theme	mannose	335:341	arg1	units					364:368	mannose (Man) monosaccharide units	335:368	mannose (Man) monosaccharide units	335:368	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	3	62	theme	viruses	678:684	arg1	infectivity					653:663	infectivity	653:663	infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2	653:713	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	3	62	theme	viruses	678:684	arg1	roles					626:630	their roles	620:630	their roles in immune escape	620:647	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	9	63	theme	binding	2079:2085	arg1	affinity					2087:2094	binding affinity	2079:2094	binding affinity to the IgG1 Fc	2079:2109	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	6	64	theme	Fc	1276:1277	arg1	FcγRIIIa					1291:1298	FcγRIIIa	1291:1298	FcγRIIIa	1291:1298	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	64	theme	Fc	1276:1277	arg1	receptor					1281:1288	the fully glycosylated CD16a Fc γ receptor	1247:1288	the fully glycosylated CD16a Fc γ receptor (FcγRIIIa)	1247:1299	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	5	65	theme	μs	959:960	arg1	results					940:946	the results	936:946	the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates	936:1119	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	3	66	theme	dynamic	513:519	arg1	nature					521:526	The highly dynamic nature	502:526	The highly dynamic nature	502:526	The highly dynamic nature, branching complexity, and 3D structure of oligomannoses have been recently highlighted for their roles in immune escape and infectivity of enveloped viruses, such as HIV-1 and SARS-CoV2.					
33661628	1	67	theme	N-glycans	147:155	arg1	class					138:142	the oldest class	127:142	the oldest class of N-glycans	127:155	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	1	67	theme	N-glycans	147:155	arg1	Oligomannoses					94:106	Oligomannoses	94:106	Oligomannoses	94:106	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	7	68	theme	hydrogen	1621:1628	arg1	networks					1638:1645	hydrogen bonding networks	1621:1645	hydrogen bonding networks	1621:1645	Our results show that the protein's structural constraints shift the oligomannoses conformational ensemble to promote conformers that satisfy the steric requirements and hydrogen bonding networks demanded by the protein's surface landscape.					
33661628	4	69	theme	disordered	841:850	arg1	nature					852:857	their intrinsically disordered nature	821:857	their intrinsically disordered nature that hinders their structural characterization	821:904	The architectural features that allow these N-glycans to perform their functions are yet unclear, due to their intrinsically disordered nature that hinders their structural characterization.					
33661628	6	70	theme	glycosylated	1257:1268	arg1	FcγRIIIa					1291:1298	FcγRIIIa	1291:1298	FcγRIIIa	1291:1298	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	70	theme	glycosylated	1257:1268	arg1	receptor					1281:1288	the fully glycosylated CD16a Fc γ receptor	1247:1288	the fully glycosylated CD16a Fc γ receptor (FcγRIIIa)	1247:1299	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	71	theme	cellular	1421:1428	arg1	ADCC					1444:1447	ADCC	1444:1447	ADCC	1444:1447	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	6	71	theme	cellular	1421:1428	arg1	cytotoxicity					1430:1441	the antibody-dependent cellular cytotoxicity	1398:1441	the antibody-dependent cellular cytotoxicity (ADCC)	1398:1448	We then discuss the effects of a protein surface on their structural equilibria based on over 4 μs cumulative MD sampling of the fully glycosylated CD16a Fc γ receptor (FcγRIIIa), where the type of glycosylation is known to modulate its binding affinity for IgG1s, regulating the antibody-dependent cellular cytotoxicity (ADCC).					
33661628	9	72	link	linked	1899:1904	arg1	glycans					1906:1912	the Man5 linked glycans	1890:1912	the Man5 linked glycans	1890:1912	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	5	73	from	vertebrates	1109:1119	arg1	common					1099:1104	common	1099:1104	common	1099:1104	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	9	74	theme	IgG1	2103:2106	arg1	Fc					2108:2109	the IgG1 Fc	2099:2109	the IgG1 Fc	2099:2109	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	2	75	theme	meanwhile	437:445	arg1	structures					447:456	meanwhile structures	437:456	meanwhile structures	437:456	In mammalian species oligomannose N-glycans can have up to 9 Man; meanwhile structures can grow to over 200 units in yeast mannan.					
33661628	9	76	theme	linked	1899:1904	arg1	glycans					1906:1912	the Man5 linked glycans	1890:1912	the Man5 linked glycans	1890:1912	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	5	77	theme	dynamics	998:1005	arg1	simulations					1012:1022	molecular dynamics (MD) simulations	988:1022	molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates	988:1119	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	5	78	dep	processed	1039:1047	arg1	free					1050:1053	free	1050:1053	free	1050:1053	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	5	78	dep	processed	1039:1047	arg1	protein-linked					1060:1073	protein-linked	1060:1073	protein-linked	1060:1073	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	1	79	dep	-GlcNAcβ1-Asn	259:271	arg1	i.e.					209:212	i.e.	209:212	i.e.	209:212	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33661628	9	80	from	presentation	2010:2021	arg1	N162					2038:2041	N162	2038:2041	N162	2038:2041	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	9	81	theme	high	1947:1950	arg1	levels					1952:1957	high levels	1947:1957	high levels of hybrid complex forms at N45	1947:1988	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	5	82	theme	oligomannose	1076:1087	arg1	N-glycans					1089:1097	differently processed, free (not protein-linked) oligomannose N-glycans	1027:1097	differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates	1027:1119	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	8	83	link	unlinked	1812:1819	arg1	forms					1821:1825	the unlinked forms	1808:1825	the unlinked forms in solution	1808:1837	More importantly, we find that the protein does not actively distort the N-glycans into structures not populated in the unlinked forms in solution.					
33661628	2	84	contain	have	419:422	arg1	N-glycans					405:413	oligomannose N-glycans	392:413	oligomannose N-glycans	392:413	In mammalian species oligomannose N-glycans can have up to 9 Man; meanwhile structures can grow to over 200 units in yeast mannan.					
33661628	2	84	contain	have	419:422	arg2	up					424:425	up	424:425	up	424:425	In mammalian species oligomannose N-glycans can have up to 9 Man; meanwhile structures can grow to over 200 units in yeast mannan.					
33661628	5	85	from	common	1099:1104	arg1	vertebrates					1109:1119	vertebrates	1109:1119	vertebrates	1109:1119	In this work we will discuss the results of over 54 μs of cumulative sampling by molecular dynamics (MD) simulations of differently processed, free (not protein-linked) oligomannose N-glycans common in vertebrates.					
33661628	9	86	theme	complex	1969:1975	arg1	forms					1977:1981	hybrid complex forms	1962:1981	hybrid complex forms at N45	1962:1988	Ultimately, the highly populated conformations of the Man5 linked glycans support experimental evidence of high levels of hybrid complex forms at N45 and show a specific presentation of the arms at N162, which may be involved in mediating binding affinity to the IgG1 Fc.					
33661628	4	87	theme	architectural	720:732	arg1	unclear					805:811	unclear	805:811	unclear	805:811	The architectural features that allow these N-glycans to perform their functions are yet unclear, due to their intrinsically disordered nature that hinders their structural characterization.					
33661628	4	87	theme	architectural	720:732	arg1	features					734:741	The architectural features	716:741	The architectural features that allow these N-glycans to perform their functions	716:795	The architectural features that allow these N-glycans to perform their functions are yet unclear, due to their intrinsically disordered nature that hinders their structural characterization.					
33661628	1	88	theme	Manα	215:218	arg1	-GlcNAcβ1-Asn					259:271	Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn	215:271	Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn	215:271	Oligomannoses are evolutionarily the oldest class of N-glycans, where the arms of the common pentasaccharide unit, i.e., Manα(1-6)-[Manα(1-3)]-Manβ(1-4)-GlcNAcβ(1-4)-GlcNAcβ1-Asn, are functionalized exclusively with branched arrangements of mannose (Man) monosaccharide units.					
33871970	2	0	theme	angiotensin-converting	444:465	arg1	enzyme					467:472	the angiotensin-converting enzyme 2	440:474	the angiotensin-converting enzyme 2 (ACE2) receptor	440:490	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	2	0	theme	angiotensin-converting	444:465	arg1	ACE2					477:480	ACE2	477:480	ACE2	477:480	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	4	1	theme	functional	662:671	arg1	characterization					673:688	an in-depth structural and functional characterization	635:688	an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells	635:783	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	2	2	with	interaction	423:433	arg1	receptor					483:490	the angiotensin-converting enzyme 2 (ACE2) receptor	440:490	the angiotensin-converting enzyme 2 (ACE2) receptor	440:490	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	1	3	theme	SARS-CoV-2	154:163	arg1	pandemic					166:173	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic	101:173	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic	101:173	As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still ongoing and dramatically influences our life, the need for recombinant viral proteins for diagnostics, vaccine development, and research is very high.					
33871970	6	4	theme	Released	965:972	arg1	glycan					974:979	Released glycan and glycopeptide analysis	965:1005	glycan	974:979	Released glycan and glycopeptide analysis were integrated with intact mass analysis, glycan-enzymatic dissection, and top-down sequencing for comprehensive annotation of RBD proteoforms.					
33871970	0	5	theme	Mammalian	81:89	arg1	Cells					91:95	Mammalian Cells	81:95	Mammalian Cells	81:95	Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells.					
33871970	8	6	theme	N-terminal	1403:1412	arg1	cleavage					1414:1421	N-terminal cleavage	1403:1421	N-terminal cleavage of the O-glycosylation	1403:1444	Additionally, using an alternative approach based on N-terminal cleavage of the O-glycosylation, the previously unknown O-glycosylation site was localized at T323.					
33871970	6	7	theme	comprehensive	1107:1119	arg1	annotation					1121:1130	comprehensive annotation	1107:1130	comprehensive annotation of RBD proteoforms	1107:1149	Released glycan and glycopeptide analysis were integrated with intact mass analysis, glycan-enzymatic dissection, and top-down sequencing for comprehensive annotation of RBD proteoforms.					
33871970	1	8	theme	recombinant	243:253	arg1	proteins					261:268	recombinant viral proteins	243:268	recombinant viral proteins	243:268	As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still ongoing and dramatically influences our life, the need for recombinant viral proteins for diagnostics, vaccine development, and research is very high.					
33871970	4	9	theme	human	743:747	arg1	kidney					759:764	human embryonic kidney 293	743:768	human embryonic kidney 293 (HEK293) cells	743:783	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	4	9	theme	human	743:747	arg1	HEK293					771:776	HEK293	771:776	HEK293	771:776	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	2	10	theme	structural	534:543	arg1	features					545:552	its structural features	530:552	its structural features	530:552	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	2	11	dep	spike	339:343	arg1	S					346:346	S	346:346	S	346:346	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	4	12	theme	hamster	719:725	arg1	CHO					734:736	CHO	734:736	CHO	734:736	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	4	12	theme	hamster	719:725	arg1	ovary					727:731	Chinese hamster ovary	711:731	Chinese hamster ovary (CHO)	711:737	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	1	13	theme	viral	255:259	arg1	proteins					261:268	recombinant viral proteins	243:268	recombinant viral proteins	243:268	As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still ongoing and dramatically influences our life, the need for recombinant viral proteins for diagnostics, vaccine development, and research is very high.					
33871970	5	14	theme	mass	923:926	arg1	approach					942:949	a multilevel mass spectrometric approach	910:949	a multilevel mass spectrometric approach	910:949	To structurally characterize the native RBDs (comprising N- and O-glycans and additional post translational modifications), a multilevel mass spectrometric approach was employed.					
33871970	7	15	theme	antenna	1242:1248	arg1	fucosylation					1250:1261	antenna fucosylation	1242:1261	antenna fucosylation	1242:1261	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	2	16	theme	spike	339:343	arg1	protein					349:355	The spike (S) protein	335:355	The spike (S) protein	335:355	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	4	17	theme	RBDs	693:696	arg1	characterization					673:688	an in-depth structural and functional characterization	635:688	an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells	635:783	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	5	18	theme	spectrometric	928:940	arg1	approach					942:949	a multilevel mass spectrometric approach	910:949	a multilevel mass spectrometric approach	910:949	To structurally characterize the native RBDs (comprising N- and O-glycans and additional post translational modifications), a multilevel mass spectrometric approach was employed.					
33871970	6	19	theme	glycan-enzymatic	1050:1065	arg1	dissection					1067:1076	glycan-enzymatic dissection	1050:1076	glycan-enzymatic dissection	1050:1076	Released glycan and glycopeptide analysis were integrated with intact mass analysis, glycan-enzymatic dissection, and top-down sequencing for comprehensive annotation of RBD proteoforms.					
33871970	6	20	theme	glycopeptide	985:996	arg1	analysis					998:1005	Released glycan and glycopeptide analysis	965:1005	analysis	998:1005	Released glycan and glycopeptide analysis were integrated with intact mass analysis, glycan-enzymatic dissection, and top-down sequencing for comprehensive annotation of RBD proteoforms.					
33871970	8	21	located	localized	1495:1503	arg2	site					1486:1489	the previously unknown O-glycosylation site	1447:1489	the previously unknown O-glycosylation site	1447:1489	Additionally, using an alternative approach based on N-terminal cleavage of the O-glycosylation, the previously unknown O-glycosylation site was localized at T323.					
33871970	8	21	located	localized	1495:1503	arg1	T323					1508:1511	T323	1508:1511	T323	1508:1511	Additionally, using an alternative approach based on N-terminal cleavage of the O-glycosylation, the previously unknown O-glycosylation site was localized at T323.					
33871970	5	22	theme	native	819:824	arg1	RBDs					826:829	the native RBDs	815:829	the native RBDs (comprising N- and O-glycans and additional post translational modifications)	815:907	To structurally characterize the native RBDs (comprising N- and O-glycans and additional post translational modifications), a multilevel mass spectrometric approach was employed.					
33871970	2	23	from	interaction	423:433	arg1	cells					500:504	host cells	495:504	host cells	495:504	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	10	24	theme	analytical	1763:1772	arg1	workflow					1774:1781	an analytical workflow	1760:1781	an analytical workflow for characterization of new RBDs and batch-to-batch comparison	1760:1844	This work not only offers insights into RBD structural and functional features but also provides an analytical workflow for characterization of new RBDs and batch-to-batch comparison.					
33871970	4	25	theme	Chinese	711:717	arg1	CHO					734:736	CHO	734:736	CHO	734:736	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	4	25	theme	Chinese	711:717	arg1	ovary					727:731	Chinese hamster ovary	711:731	Chinese hamster ovary (CHO)	711:737	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	7	26	theme	higher	1266:1271	arg1	level					1273:1277	a higher level	1264:1277	a higher level of sialylation	1264:1292	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	9	27	theme	positive	1569:1576	arg1	patients					1578:1585	positive patients	1569:1585	positive patients	1569:1585	For both RBDs, the binding to SARS-CoV-2 antibodies of positive patients and affinity to the ACE2 receptor was addressed showing comparable results.					
33871970	0	28	theme	Structural	0:9	arg1	Characterization					26:41	Structural and Functional Characterization	0:41	Structural and Functional Characterization of SARS-CoV-2 RBD Domains	0:67	Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells.					
33871970	10	29	theme	RBDs	1811:1814	arg1	characterization					1787:1802	characterization	1787:1802	characterization of new RBDs	1787:1814	This work not only offers insights into RBD structural and functional features but also provides an analytical workflow for characterization of new RBDs and batch-to-batch comparison.					
33871970	10	29	theme	RBDs	1811:1814	arg1	comparison					1835:1844	batch-to-batch comparison	1820:1844	batch-to-batch comparison	1820:1844	This work not only offers insights into RBD structural and functional features but also provides an analytical workflow for characterization of new RBDs and batch-to-batch comparison.					
33871970	6	30	theme	proteoforms	1139:1149	arg1	annotation					1121:1130	comprehensive annotation	1107:1130	comprehensive annotation of RBD proteoforms	1107:1149	Released glycan and glycopeptide analysis were integrated with intact mass analysis, glycan-enzymatic dissection, and top-down sequencing for comprehensive annotation of RBD proteoforms.					
33871970	7	31	theme	type	1334:1337	arg1	O-glycans					1339:1347	core 1 and core 2 type O-glycans	1316:1347	O-glycans	1339:1347	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	6	32	theme	mass	1035:1038	arg1	analysis					1040:1047	intact mass analysis	1028:1047	intact mass analysis	1028:1047	Released glycan and glycopeptide analysis were integrated with intact mass analysis, glycan-enzymatic dissection, and top-down sequencing for comprehensive annotation of RBD proteoforms.					
33871970	5	33	theme	translational	880:892	arg1	modifications					894:906	additional post translational modifications	864:906	additional post translational modifications	864:906	To structurally characterize the native RBDs (comprising N- and O-glycans and additional post translational modifications), a multilevel mass spectrometric approach was employed.					
33871970	6	34	theme	RBD	1135:1137	arg1	proteoforms					1139:1149	RBD proteoforms	1135:1149	RBD proteoforms	1135:1149	Released glycan and glycopeptide analysis were integrated with intact mass analysis, glycan-enzymatic dissection, and top-down sequencing for comprehensive annotation of RBD proteoforms.					
33871970	7	35	with	glycosylation	1177:1189	arg1	latter					1224:1229	latter	1224:1229	latter	1224:1229	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	5	36	theme	multilevel	912:921	arg1	approach					942:949	a multilevel mass spectrometric approach	910:949	a multilevel mass spectrometric approach	910:949	To structurally characterize the native RBDs (comprising N- and O-glycans and additional post translational modifications), a multilevel mass spectrometric approach was employed.					
33871970	0	37	theme	Functional	15:24	arg1	Characterization					26:41	Structural and Functional Characterization	0:41	Structural and Functional Characterization of SARS-CoV-2 RBD Domains	0:67	Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells.					
33871970	7	38	gly	fucosylation	1250:1261	arg1	sialylation					1282:1292	sialylation	1282:1292	sialylation	1282:1292	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	7	38	gly	fucosylation	1250:1261	arg1	core					1316:1319	core 1 and core 2 type O-glycans	1316:1347	core	1316:1319	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	7	38	gly	fucosylation	1250:1261	arg1	O-glycans					1339:1347	core 1 and core 2 type O-glycans	1316:1347	O-glycans	1339:1347	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	1	39	theme	severe	105:110	arg1	syndrome					130:137	the severe acute respiratory syndrome	101:137	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic	101:173	As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still ongoing and dramatically influences our life, the need for recombinant viral proteins for diagnostics, vaccine development, and research is very high.					
33871970	2	40	theme	receptor-binding	379:394	arg1	RBD					404:406	RBD	404:406	RBD	404:406	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	2	40	theme	receptor-binding	379:394	arg1	domain					396:401	its receptor-binding domain	375:401	its receptor-binding domain (RBD)	375:407	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	0	41	theme	SARS-CoV-2	46:55	arg1	Domains					61:67	SARS-CoV-2 RBD Domains	46:67	SARS-CoV-2 RBD Domains	46:67	Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells.					
33871970	1	42	theme	vaccine	287:293	arg1	development					295:305	vaccine development	287:305	vaccine development	287:305	As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still ongoing and dramatically influences our life, the need for recombinant viral proteins for diagnostics, vaccine development, and research is very high.					
33871970	2	43	theme	host	495:498	arg1	cells					500:504	host cells	495:504	host cells	495:504	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	7	44	theme	distinct	1168:1175	arg1	glycosylation					1177:1189	distinct glycosylation	1168:1189	distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans	1168:1347	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	5	45	dep	RBDs	826:829	arg1	comprising					832:841	comprising	832:841	comprising N- and O-glycans and additional post translational modifications	832:906	To structurally characterize the native RBDs (comprising N- and O-glycans and additional post translational modifications), a multilevel mass spectrometric approach was employed.					
33871970	7	46	theme	sialylation	1282:1292	arg1	fucosylation					1250:1261	antenna fucosylation	1242:1261	antenna fucosylation	1242:1261	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	7	46	theme	sialylation	1282:1292	arg1	level					1273:1277	a higher level	1264:1277	a higher level of sialylation	1264:1292	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	7	46	theme	sialylation	1282:1292	arg1	combination					1301:1311	a combination	1299:1311	a combination of core 1 and core 2 type O-glycans	1299:1347	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	7	47	theme	core	1316:1319	arg1	fucosylation					1250:1261	antenna fucosylation	1242:1261	antenna fucosylation	1242:1261	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	7	47	theme	core	1316:1319	arg1	level					1273:1277	a higher level	1264:1277	a higher level of sialylation	1264:1292	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	7	47	theme	core	1316:1319	arg1	combination					1301:1311	a combination	1299:1311	a combination of core 1 and core 2 type O-glycans	1299:1347	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	5	48	theme	additional	864:873	arg1	modifications					894:906	additional post translational modifications	864:906	additional post translational modifications	864:906	To structurally characterize the native RBDs (comprising N- and O-glycans and additional post translational modifications), a multilevel mass spectrometric approach was employed.					
33871970	8	49	gly	O-glycosylation	1470:1484	arg2	site					1486:1489	the previously unknown O-glycosylation site	1447:1489	the previously unknown O-glycosylation site	1447:1489	Additionally, using an alternative approach based on N-terminal cleavage of the O-glycosylation, the previously unknown O-glycosylation site was localized at T323.					
33871970	0	50	theme	Domains	61:67	arg1	Characterization					26:41	Structural and Functional Characterization	0:41	Structural and Functional Characterization of SARS-CoV-2 RBD Domains	0:67	Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells.					
33871970	10	51	theme	structural	1707:1716	arg1	features					1733:1740	RBD structural and functional features	1703:1740	RBD structural and functional features	1703:1740	This work not only offers insights into RBD structural and functional features but also provides an analytical workflow for characterization of new RBDs and batch-to-batch comparison.					
33871970	8	52	theme	O-glycosylation	1430:1444	arg1	cleavage					1414:1421	N-terminal cleavage	1403:1421	N-terminal cleavage of the O-glycosylation	1403:1444	Additionally, using an alternative approach based on N-terminal cleavage of the O-glycosylation, the previously unknown O-glycosylation site was localized at T323.					
33871970	7	53	theme	O-glycans	1339:1347	arg1	fucosylation					1250:1261	antenna fucosylation	1242:1261	antenna fucosylation	1242:1261	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	7	53	theme	O-glycans	1339:1347	arg1	level					1273:1277	a higher level	1264:1277	a higher level of sialylation	1264:1292	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	7	53	theme	O-glycans	1339:1347	arg1	combination					1301:1311	a combination	1299:1311	a combination of core 1 and core 2 type O-glycans	1299:1347	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	6	54	theme	top-down	1083:1090	arg1	sequencing					1092:1101	top-down sequencing	1083:1101	top-down sequencing	1083:1101	Released glycan and glycopeptide analysis were integrated with intact mass analysis, glycan-enzymatic dissection, and top-down sequencing for comprehensive annotation of RBD proteoforms.					
33871970	1	55	theme	acute	112:116	arg1	syndrome					130:137	the severe acute respiratory syndrome	101:137	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic	101:173	As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still ongoing and dramatically influences our life, the need for recombinant viral proteins for diagnostics, vaccine development, and research is very high.					
33871970	0	56	theme	RBD	57:59	arg1	Domains					61:67	SARS-CoV-2 RBD Domains	46:67	SARS-CoV-2 RBD Domains	46:67	Structural and Functional Characterization of SARS-CoV-2 RBD Domains Produced in Mammalian Cells.					
33871970	8	57	theme	alternative	1373:1383	arg1	approach					1385:1392	an alternative approach	1370:1392	an alternative approach based on N-terminal cleavage of the O-glycosylation	1370:1444	Additionally, using an alternative approach based on N-terminal cleavage of the O-glycosylation, the previously unknown O-glycosylation site was localized at T323.					
33871970	9	58	theme	comparable	1643:1652	arg1	results					1654:1660	comparable results	1643:1660	comparable results	1643:1660	For both RBDs, the binding to SARS-CoV-2 antibodies of positive patients and affinity to the ACE2 receptor was addressed showing comparable results.					
33871970	1	59	theme	respiratory	118:128	arg1	syndrome					130:137	the severe acute respiratory syndrome	101:137	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic	101:173	As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still ongoing and dramatically influences our life, the need for recombinant viral proteins for diagnostics, vaccine development, and research is very high.					
33871970	7	60	theme	core	1327:1330	arg1	O-glycans					1339:1347	core 1 and core 2 type O-glycans	1316:1347	O-glycans	1339:1347	The data showed distinct glycosylation for CHO- and HEK293-RBD with the latter exhibiting antenna fucosylation, a higher level of sialylation, and a combination of core 1 and core 2 type O-glycans.					
33871970	4	61	theme	embryonic	749:757	arg1	kidney					759:764	human embryonic kidney 293	743:768	human embryonic kidney 293 (HEK293) cells	743:783	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	4	61	theme	embryonic	749:757	arg1	HEK293					771:776	HEK293	771:776	HEK293	771:776	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	3	62	theme	well-characterized	566:583	arg1	RBDs					597:600	well-characterized recombinant RBDs	566:600	well-characterized recombinant RBDs	566:600	Therefore, well-characterized recombinant RBDs are essential.					
33871970	9	63	theme	patients	1578:1585	arg1	affinity					1591:1598	affinity	1591:1598	affinity to the ACE2 receptor	1591:1619	For both RBDs, the binding to SARS-CoV-2 antibodies of positive patients and affinity to the ACE2 receptor was addressed showing comparable results.					
33871970	9	63	theme	patients	1578:1585	arg1	binding					1533:1539	the binding	1529:1539	the binding to SARS-CoV-2 antibodies of positive patients	1529:1585	For both RBDs, the binding to SARS-CoV-2 antibodies of positive patients and affinity to the ACE2 receptor was addressed showing comparable results.					
33871970	1	64	theme	syndrome	130:137	arg1	pandemic					166:173	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic	101:173	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic	101:173	As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still ongoing and dramatically influences our life, the need for recombinant viral proteins for diagnostics, vaccine development, and research is very high.					
33871970	10	65	theme	RBD	1703:1705	arg1	features					1733:1740	RBD structural and functional features	1703:1740	RBD structural and functional features	1703:1740	This work not only offers insights into RBD structural and functional features but also provides an analytical workflow for characterization of new RBDs and batch-to-batch comparison.					
33871970	10	66	theme	functional	1722:1731	arg1	features					1733:1740	RBD structural and functional features	1703:1740	RBD structural and functional features	1703:1740	This work not only offers insights into RBD structural and functional features but also provides an analytical workflow for characterization of new RBDs and batch-to-batch comparison.					
33871970	8	67	theme	O-glycosylation	1470:1484	arg1	site					1486:1489	the previously unknown O-glycosylation site	1447:1489	the previously unknown O-glycosylation site	1447:1489	Additionally, using an alternative approach based on N-terminal cleavage of the O-glycosylation, the previously unknown O-glycosylation site was localized at T323.					
33871970	3	68	theme	recombinant	585:595	arg1	RBDs					597:600	well-characterized recombinant RBDs	566:600	well-characterized recombinant RBDs	566:600	Therefore, well-characterized recombinant RBDs are essential.					
33871970	6	69	gly	glycopeptide	985:996	arg2	glycopeptide					985:996	Released glycan and glycopeptide analysis	965:1005	glycopeptide	985:996	Released glycan and glycopeptide analysis were integrated with intact mass analysis, glycan-enzymatic dissection, and top-down sequencing for comprehensive annotation of RBD proteoforms.					
33871970	1	70	theme	coronavirus	139:149	arg1	pandemic					166:173	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic	101:173	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic	101:173	As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is still ongoing and dramatically influences our life, the need for recombinant viral proteins for diagnostics, vaccine development, and research is very high.					
33871970	5	71	theme	post	875:878	arg1	modifications					894:906	additional post translational modifications	864:906	additional post translational modifications	864:906	To structurally characterize the native RBDs (comprising N- and O-glycans and additional post translational modifications), a multilevel mass spectrometric approach was employed.					
33871970	8	72	theme	unknown	1462:1468	arg1	site					1486:1489	the previously unknown O-glycosylation site	1447:1489	the previously unknown O-glycosylation site	1447:1489	Additionally, using an alternative approach based on N-terminal cleavage of the O-glycosylation, the previously unknown O-glycosylation site was localized at T323.					
33871970	9	73	theme	ACE2	1607:1610	arg1	receptor					1612:1619	the ACE2 receptor	1603:1619	the ACE2 receptor	1603:1619	For both RBDs, the binding to SARS-CoV-2 antibodies of positive patients and affinity to the ACE2 receptor was addressed showing comparable results.					
33871970	6	74	theme	intact	1028:1033	arg1	analysis					1040:1047	intact mass analysis	1028:1047	intact mass analysis	1028:1047	Released glycan and glycopeptide analysis were integrated with intact mass analysis, glycan-enzymatic dissection, and top-down sequencing for comprehensive annotation of RBD proteoforms.					
33871970	4	75	theme	structural	647:656	arg1	characterization					673:688	an in-depth structural and functional characterization	635:688	an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells	635:783	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	9	76	theme	SARS-CoV-2	1544:1553	arg1	antibodies					1555:1564	SARS-CoV-2 antibodies	1544:1564	SARS-CoV-2 antibodies	1544:1564	For both RBDs, the binding to SARS-CoV-2 antibodies of positive patients and affinity to the ACE2 receptor was addressed showing comparable results.					
33871970	2	77	theme	enzyme	467:472	arg1	receptor					483:490	the angiotensin-converting enzyme 2 (ACE2) receptor	440:490	the angiotensin-converting enzyme 2 (ACE2) receptor	440:490	The spike (S) protein, and particularly its receptor-binding domain (RBD), mediates the interaction with the angiotensin-converting enzyme 2 (ACE2) receptor on host cells and may be modulated by its structural features.					
33871970	4	78	theme	kidney	759:764	arg1	cells					779:783	human embryonic kidney 293 (HEK293) cells	743:783	human embryonic kidney 293 (HEK293) cells	743:783	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	10	79	theme	new	1807:1809	arg1	RBDs					1811:1814	new RBDs	1807:1814	new RBDs	1807:1814	This work not only offers insights into RBD structural and functional features but also provides an analytical workflow for characterization of new RBDs and batch-to-batch comparison.					
33871970	4	80	theme	in-depth	638:645	arg1	characterization					673:688	an in-depth structural and functional characterization	635:688	an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells	635:783	We have performed an in-depth structural and functional characterization of RBDs expressed in Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells.					
33871970	10	81	theme	batch-to-batch	1820:1833	arg1	comparison					1835:1844	batch-to-batch comparison	1820:1844	batch-to-batch comparison	1820:1844	This work not only offers insights into RBD structural and functional features but also provides an analytical workflow for characterization of new RBDs and batch-to-batch comparison.					
33777069	8	0	theme	Lewis	1231:1235	arg1	A					1237:1237	Lewis A	1231:1237	Lewis A	1231:1237	Selected identified proteins were recombinantly expressed and the presence of Lewis A confirmed via immunoblotting and site-specific N-glycan analysis.					
33777069	9	1	dep	O.	1338:1339	arg1	sativa					1341:1346	O. sativa	1338:1346	O. sativa	1338:1346	While the proteins identified in O. sativa are associated with diverse functions, proteins from A. thaliana and N. benthamiana are mainly involved in cell wall biosynthesis.					
33777069	6	2	theme	plant	864:868	arg1	sativa					934:939	Oryza sativa	928:939	Oryza sativa	928:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	2	theme	plant	864:868	arg1	species					870:876	three different plant species	848:876	three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa	848:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	2	theme	plant	864:868	arg1	thaliana					891:898	Arabidopsis thaliana	879:898	Arabidopsis thaliana	879:898	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	2	theme	plant	864:868	arg1	benthamiana					911:921	Nicotiana benthamiana	901:921	Nicotiana benthamiana	901:921	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	12	3	attach	present	1906:1912	arg2	modification					1889:1900	the modification	1885:1900	the modification	1885:1900	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	12	3	attach	present	1906:1912	arg2	present					1906:1912	present	1906:1912	present	1906:1912	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	12	3	attach	present	1906:1912	arg1	accessions					1934:1943	all other tested accessions	1917:1943	all other tested accessions	1917:1943	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	11	4	theme	genome	1721:1726	arg1	database					1728:1735	the 1001 genome database	1712:1735	the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis	1712:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	10	5	theme	A-deficient	1496:1506	arg1	line					1515:1518	a Lewis A-deficient mutant line	1488:1518	a Lewis A-deficient mutant line of A. thaliana	1488:1533	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	2	6	theme	complex-type	275:286	arg1	N-glycans					288:296	complex-type N-glycans	275:296	complex-type N-glycans	275:296	Terminal sugar residues on complex-type N-glycans mediate various crucial biological processes in mammals such as cell-cell recognition or protein-ligand interactions.					
33777069	1	7	theme	protein	195:201	arg1	N-glycosylation					158:172	N-glycosylation	158:172	N-glycosylation	158:172	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	1	7	theme	protein	195:201	arg1	modification					203:214	a highly abundant protein modification	177:214	a highly abundant protein modification present in all domains of life	177:245	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	8	8	theme	site-specific	1272:1284	arg1	analysis					1295:1302	site-specific N-glycan analysis	1272:1302	site-specific N-glycan analysis	1272:1302	Selected identified proteins were recombinantly expressed and the presence of Lewis A confirmed via immunoblotting and site-specific N-glycan analysis.					
33777069	2	9	theme	crucial	314:320	arg1	processes					333:341	various crucial biological processes	306:341	various crucial biological processes	306:341	Terminal sugar residues on complex-type N-glycans mediate various crucial biological processes in mammals such as cell-cell recognition or protein-ligand interactions.					
33777069	1	10	theme	present	216:222	arg1	N-glycosylation					158:172	N-glycosylation	158:172	N-glycosylation	158:172	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	1	10	theme	present	216:222	arg1	modification					203:214	a highly abundant protein modification	177:214	a highly abundant protein modification present in all domains of life	177:245	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	6	11	gly	glycoproteins	829:841	arg1	glycoproteins					829:841	glycoproteins	829:841	glycoproteins	829:841	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	12	dep	species	870:876	arg1	sativa					934:939	Oryza sativa	928:939	Oryza sativa	928:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	12	dep	species	870:876	arg1	species					870:876	three different plant species	848:876	three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa	848:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	12	dep	species	870:876	arg1	thaliana					891:898	Arabidopsis thaliana	879:898	Arabidopsis thaliana	879:898	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	12	dep	species	870:876	arg1	benthamiana					911:921	Nicotiana benthamiana	901:921	Nicotiana benthamiana	901:921	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	0	13	theme	Arabidopsis	125:135	arg1	Accessions					146:155	Natural Arabidopsis thaliana Accessions	117:155	Natural Arabidopsis thaliana Accessions	117:155	Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions.					
33777069	12	14	from	present	1906:1912	arg1	accessions					1934:1943	all other tested accessions	1917:1943	all other tested accessions	1917:1943	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	1	15	from	domains	231:237	arg1	present					216:222	present	216:222	present	216:222	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	13	16	from	role	2114:2117	arg1	plants					2144:2149	plants	2144:2149	plants	2144:2149	The data provided here comprises the so far first attempt at identifying Lewis A bearing glycoproteins across different species and will help to shed more light on the role of Lewis A structures in plants.					
33777069	5	17	theme	N-glycan	731:738	arg1	modification					740:751	this complex N-glycan modification	718:751	this complex N-glycan modification	718:751	Despite their ubiquitous occurrence, the biological function of this complex N-glycan modification is currently unknown.					
33777069	13	18	theme	A	2128:2128	arg1	structures					2130:2139	Lewis A structures	2122:2139	Lewis A structures	2122:2139	The data provided here comprises the so far first attempt at identifying Lewis A bearing glycoproteins across different species and will help to shed more light on the role of Lewis A structures in plants.					
33777069	11	19	theme	A	1804:1804	arg1	biosynthesis					1806:1817	Lewis A biosynthesis	1798:1817	Lewis A biosynthesis	1798:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	11	20	attach	presence	1653:1660	arg2	structures					1673:1682	Lewis A structures	1665:1682	Lewis A structures	1665:1682	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	11	20	attach	presence	1653:1660	arg1	accessions					1696:1705	selected accessions	1687:1705	selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis	1687:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	2	21	theme	Terminal	248:255	arg1	residues					263:270	Terminal sugar residues	248:270	Terminal sugar residues on complex-type N-glycans	248:296	Terminal sugar residues on complex-type N-glycans mediate various crucial biological processes in mammals such as cell-cell recognition or protein-ligand interactions.					
33777069	7	22	theme	plant	1035:1039	arg1	N-glycans					1041:1049	complex plant N-glycans	1027:1049	complex plant N-glycans	1027:1049	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS.					
33777069	12	23	located	present	1906:1912	arg2	modification					1889:1900	the modification	1885:1900	the modification	1885:1900	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	12	23	located	present	1906:1912	arg2	present					1906:1912	present	1906:1912	present	1906:1912	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	12	23	located	present	1906:1912	arg1	accessions					1934:1943	all other tested accessions	1917:1943	all other tested accessions	1917:1943	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	0	24	theme	Wall	57:60	arg1	Biosynthesis					62:73	Cell Wall Biosynthesis	52:73	Cell Wall Biosynthesis	52:73	Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions.					
33777069	4	25	dep	conserved	582:590	arg1	evolutionary					569:580	evolutionary	569:580	evolutionary	569:580	Lewis A containing complex N-glycans appear evolutionary conserved, having been identified in all plant species analyzed so far.					
33777069	7	26	theme	A	1011:1011	arg1	structures					1013:1022	Lewis A structures	1005:1022	Lewis A structures on complex plant N-glycans	1005:1049	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS.					
33777069	4	27	theme	Lewis	525:529	arg1	A					531:531	Lewis A	525:531	Lewis A containing complex N-glycans	525:560	Lewis A containing complex N-glycans appear evolutionary conserved, having been identified in all plant species analyzed so far.					
33777069	0	28	theme	Lewis	0:4	arg1	Glycans					8:14	Lewis A Glycans	0:14	Lewis A Glycans	0:14	Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions.					
33777069	10	29	theme	cell	1568:1571	arg1	lignin					1612:1617	lignin	1612:1617	lignin	1612:1617	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	10	29	theme	cell	1568:1571	arg1	constituents					1578:1589	cell wall constituents	1568:1589	cell wall constituents such as cellulose or lignin	1568:1617	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	10	29	theme	cell	1568:1571	arg1	cellulose					1599:1607	cellulose	1599:1607	cellulose	1599:1607	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	6	30	theme	Oryza	928:932	arg1	sativa					934:939	Oryza sativa	928:939	Oryza sativa	928:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	30	theme	Oryza	928:932	arg1	species					870:876	three different plant species	848:876	three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa	848:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	3	31	theme	A	437:437	arg1	trisaccharide					439:451	the Lewis A trisaccharide	427:451	the Lewis A trisaccharide	427:451	In plants, the Lewis A trisaccharide constitutes the only known outer-chain elongation of complex N-glycans.					
33777069	3	32	theme	outer-chain	480:490	arg1	elongation					492:501	the only known outer-chain elongation	465:501	the only known outer-chain elongation of complex N-glycans	465:522	In plants, the Lewis A trisaccharide constitutes the only known outer-chain elongation of complex N-glycans.					
33777069	12	33	theme	tested	1927:1932	arg1	accessions					1934:1943	all other tested accessions	1917:1943	all other tested accessions	1917:1943	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	12	34	theme	A	1882:1882	arg1	levels					1866:1871	no detectable levels	1852:1871	no detectable levels of Lewis A	1852:1882	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	6	35	theme	Lewis	813:817	arg1	A					819:819	Lewis A	813:819	Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa	813:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	2	36	theme	cell-cell	362:370	arg1	recognition					372:382	cell-cell recognition	362:382	cell-cell recognition	362:382	Terminal sugar residues on complex-type N-glycans mediate various crucial biological processes in mammals such as cell-cell recognition or protein-ligand interactions.					
33777069	8	37	theme	identified	1162:1171	arg1	proteins					1173:1180	identified proteins	1162:1180	Selected identified proteins	1153:1180	Selected identified proteins were recombinantly expressed and the presence of Lewis A confirmed via immunoblotting and site-specific N-glycan analysis.					
33777069	6	38	theme	Nicotiana	901:909	arg1	species					870:876	three different plant species	848:876	three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa	848:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	38	theme	Nicotiana	901:909	arg1	benthamiana					911:921	Nicotiana benthamiana	901:921	Nicotiana benthamiana	901:921	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	10	39	theme	constituents	1578:1589	arg1	abundance					1555:1563	abundance	1555:1563	abundance of cell wall constituents such as cellulose or lignin	1555:1617	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	11	40	theme	Lewis	1798:1802	arg1	A					1804:1804	Lewis A	1798:1804	Lewis A biosynthesis	1798:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	3	41	theme	N-glycans	514:522	arg1	elongation					492:501	the only known outer-chain elongation	465:501	the only known outer-chain elongation of complex N-glycans	465:522	In plants, the Lewis A trisaccharide constitutes the only known outer-chain elongation of complex N-glycans.					
33777069	9	42	theme	wall	1460:1463	arg1	biosynthesis					1465:1476	cell wall biosynthesis	1455:1476	cell wall biosynthesis	1455:1476	While the proteins identified in O. sativa are associated with diverse functions, proteins from A. thaliana and N. benthamiana are mainly involved in cell wall biosynthesis.					
33777069	12	43	theme	detectable	1855:1864	arg1	levels					1866:1871	no detectable levels	1852:1871	no detectable levels of Lewis A	1852:1882	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	11	44	theme	selected	1687:1694	arg1	accessions					1696:1705	selected accessions	1687:1705	selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis	1687:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	1	45	theme	life	242:245	arg1	life					242:245	life	242:245	life	242:245	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	1	45	theme	life	242:245	arg1	domains					231:237	all domains	227:237	all domains of life	227:245	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	11	46	theme	1001	1716:1719	arg1	database					1728:1735	the 1001 genome database	1712:1735	the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis	1712:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	5	47	theme	biological	695:704	arg1	function					706:713	the biological function	691:713	the biological function of this complex N-glycan modification	691:751	Despite their ubiquitous occurrence, the biological function of this complex N-glycan modification is currently unknown.					
33777069	5	47	theme	biological	695:704	arg1	unknown					766:772	unknown	766:772	unknown	766:772	Despite their ubiquitous occurrence, the biological function of this complex N-glycan modification is currently unknown.					
33777069	11	48	theme	structures	1673:1682	arg1	presence					1653:1660	the presence	1649:1660	the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis	1649:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	4	49	contain	containing	533:542	arg1	A					531:531	Lewis A	525:531	Lewis A containing complex N-glycans	525:560	Lewis A containing complex N-glycans appear evolutionary conserved, having been identified in all plant species analyzed so far.					
33777069	4	49	contain	containing	533:542	arg2	N-glycans					552:560	complex N-glycans	544:560	complex N-glycans	544:560	Lewis A containing complex N-glycans appear evolutionary conserved, having been identified in all plant species analyzed so far.					
33777069	12	50	theme	relict	1832:1837	arg1	line					1839:1842	one relict line	1828:1842	one relict line showing no detectable levels of Lewis A	1828:1882	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	4	51	theme	plant	623:627	arg1	species					629:635	all plant species	619:635	all plant species analyzed so far	619:651	Lewis A containing complex N-glycans appear evolutionary conserved, having been identified in all plant species analyzed so far.					
33777069	13	52	theme	first	1990:1994	arg1	attempt					1996:2002	the so far first attempt	1979:2002	the so far first attempt at identifying Lewis A bearing glycoproteins across different species	1979:2072	The data provided here comprises the so far first attempt at identifying Lewis A bearing glycoproteins across different species and will help to shed more light on the role of Lewis A structures in plants.					
33777069	13	53	theme	Lewis	2019:2023	arg1	A					2025:2025	Lewis A	2019:2025	Lewis A bearing glycoproteins	2019:2047	The data provided here comprises the so far first attempt at identifying Lewis A bearing glycoproteins across different species and will help to shed more light on the role of Lewis A structures in plants.					
33777069	2	54	theme	various	306:312	arg1	processes					333:341	various crucial biological processes	306:341	various crucial biological processes	306:341	Terminal sugar residues on complex-type N-glycans mediate various crucial biological processes in mammals such as cell-cell recognition or protein-ligand interactions.					
33777069	13	55	gly	glycoproteins	2035:2047	arg1	glycoproteins					2035:2047	glycoproteins	2035:2047	glycoproteins	2035:2047	The data provided here comprises the so far first attempt at identifying Lewis A bearing glycoproteins across different species and will help to shed more light on the role of Lewis A structures in plants.					
33777069	11	56	theme	Lewis	1665:1669	arg1	structures					1673:1682	Lewis A structures	1665:1682	Lewis A structures	1665:1682	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	11	57	theme	amino	1748:1752	arg1	variations					1759:1768	amino acid variations	1748:1768	amino acid variations in the enzymes required for Lewis A biosynthesis	1748:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	5	58	theme	ubiquitous	668:677	arg1	occurrence					679:688	their ubiquitous occurrence	662:688	their ubiquitous occurrence	662:688	Despite their ubiquitous occurrence, the biological function of this complex N-glycan modification is currently unknown.					
33777069	6	59	theme	different	854:862	arg1	sativa					934:939	Oryza sativa	928:939	Oryza sativa	928:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	59	theme	different	854:862	arg1	species					870:876	three different plant species	848:876	three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa	848:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	59	theme	different	854:862	arg1	thaliana					891:898	Arabidopsis thaliana	879:898	Arabidopsis thaliana	879:898	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	59	theme	different	854:862	arg1	benthamiana					911:921	Nicotiana benthamiana	901:921	Nicotiana benthamiana	901:921	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	8	60	theme	A	1237:1237	arg1	presence					1219:1226	the presence	1215:1226	the presence of Lewis A	1215:1237	Selected identified proteins were recombinantly expressed and the presence of Lewis A confirmed via immunoblotting and site-specific N-glycan analysis.					
33777069	0	61	attach	Present	20:26	arg1	Proteins					31:38	Proteins	31:38	Proteins Involved in Cell Wall Biosynthesis	31:73	Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions.					
33777069	0	61	attach	Present	20:26	arg2	Glycans					8:14	Lewis A Glycans	0:14	Lewis A Glycans	0:14	Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions.					
33777069	10	62	theme	Lewis	1490:1494	arg1	line					1515:1518	a Lewis A-deficient mutant line	1488:1518	a Lewis A-deficient mutant line of A. thaliana	1488:1533	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	1	63	theme	abundant	186:193	arg1	N-glycosylation					158:172	N-glycosylation	158:172	N-glycosylation	158:172	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	1	63	theme	abundant	186:193	arg1	modification					203:214	a highly abundant protein modification	177:214	a highly abundant protein modification present in all domains of life	177:245	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	9	64	from	thaliana	1404:1411	arg1	proteins					1387:1394	proteins	1387:1394	proteins from A. thaliana and N. benthamiana	1387:1430	While the proteins identified in O. sativa are associated with diverse functions, proteins from A. thaliana and N. benthamiana are mainly involved in cell wall biosynthesis.					
33777069	12	65	from	accessions	1934:1943	arg1	present					1906:1912	present	1906:1912	present	1906:1912	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	12	65	from	accessions	1934:1943	arg1	modification					1889:1900	the modification	1885:1900	the modification	1885:1900	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	0	66	theme	Natural	117:123	arg1	Accessions					146:155	Natural Arabidopsis thaliana Accessions	117:155	Natural Arabidopsis thaliana Accessions	117:155	Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions.					
33777069	2	67	theme	biological	322:331	arg1	processes					333:341	various crucial biological processes	306:341	various crucial biological processes	306:341	Terminal sugar residues on complex-type N-glycans mediate various crucial biological processes in mammals such as cell-cell recognition or protein-ligand interactions.					
33777069	0	68	theme	thaliana	137:144	arg1	Accessions					146:155	Natural Arabidopsis thaliana Accessions	117:155	Natural Arabidopsis thaliana Accessions	117:155	Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions.					
33777069	8	69	theme	N-glycan	1286:1293	arg1	analysis					1295:1302	site-specific N-glycan analysis	1272:1302	site-specific N-glycan analysis	1272:1302	Selected identified proteins were recombinantly expressed and the presence of Lewis A confirmed via immunoblotting and site-specific N-glycan analysis.					
33777069	5	70	theme	complex	723:729	arg1	modification					740:751	this complex N-glycan modification	718:751	this complex N-glycan modification	718:751	Despite their ubiquitous occurrence, the biological function of this complex N-glycan modification is currently unknown.					
33777069	7	71	theme	Lewis	1071:1075	arg1	A					1077:1077	Lewis A	1071:1077	Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS	1071:1150	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS.					
33777069	10	72	from	change	1545:1550	arg1	abundance					1555:1563	abundance	1555:1563	abundance of cell wall constituents such as cellulose or lignin	1555:1617	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	7	73	gly	glycoproteins	1087:1099	arg1	glycoproteins					1087:1099	glycoproteins	1087:1099	glycoproteins	1087:1099	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS.					
33777069	5	74	theme	modification	740:751	arg1	function					706:713	the biological function	691:713	the biological function of this complex N-glycan modification	691:751	Despite their ubiquitous occurrence, the biological function of this complex N-glycan modification is currently unknown.					
33777069	5	74	theme	modification	740:751	arg1	unknown					766:772	unknown	766:772	unknown	766:772	Despite their ubiquitous occurrence, the biological function of this complex N-glycan modification is currently unknown.					
33777069	13	75	theme	structures	2130:2139	arg1	role					2114:2117	the role	2110:2117	the role of Lewis A structures in plants	2110:2149	The data provided here comprises the so far first attempt at identifying Lewis A bearing glycoproteins across different species and will help to shed more light on the role of Lewis A structures in plants.					
33777069	1	76	from	present	216:222	arg1	life					242:245	life	242:245	life	242:245	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	1	76	from	present	216:222	arg1	domains					231:237	all domains	227:237	all domains of life	227:245	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	7	77	used	used	1056:1059	arg2	purification					951:962	Affinity purification	942:962	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans,	942:1050	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS.					
33777069	0	78	theme	Cell	52:55	arg1	Biosynthesis					62:73	Cell Wall Biosynthesis	52:73	Cell Wall Biosynthesis	52:73	Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions.					
33777069	2	79	theme	sugar	257:261	arg1	residues					263:270	Terminal sugar residues	248:270	Terminal sugar residues on complex-type N-glycans	248:296	Terminal sugar residues on complex-type N-glycans mediate various crucial biological processes in mammals such as cell-cell recognition or protein-ligand interactions.					
33777069	4	80	theme	complex	544:550	arg1	N-glycans					552:560	complex N-glycans	544:560	complex N-glycans	544:560	Lewis A containing complex N-glycans appear evolutionary conserved, having been identified in all plant species analyzed so far.					
33777069	7	81	from	structures	1013:1022	arg1	N-glycans					1041:1049	complex plant N-glycans	1027:1049	complex plant N-glycans	1027:1049	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS.					
33777069	9	82	theme	diverse	1368:1374	arg1	functions					1376:1384	diverse functions	1368:1384	diverse functions	1368:1384	While the proteins identified in O. sativa are associated with diverse functions, proteins from A. thaliana and N. benthamiana are mainly involved in cell wall biosynthesis.					
33777069	13	83	theme	Lewis	2122:2126	arg1	structures					2130:2139	Lewis A structures	2122:2139	Lewis A structures	2122:2139	The data provided here comprises the so far first attempt at identifying Lewis A bearing glycoproteins across different species and will help to shed more light on the role of Lewis A structures in plants.					
33777069	7	84	theme	complex	1027:1033	arg1	N-glycans					1041:1049	complex plant N-glycans	1027:1049	complex plant N-glycans	1027:1049	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS.					
33777069	11	85	from	variations	1759:1768	arg1	enzymes					1777:1783	the enzymes	1773:1783	the enzymes required for Lewis A biosynthesis	1773:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	10	86	theme	mutant	1508:1513	arg1	line					1515:1518	a Lewis A-deficient mutant line	1488:1518	a Lewis A-deficient mutant line of A. thaliana	1488:1533	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	9	87	from	benthamiana	1420:1430	arg1	proteins					1387:1394	proteins	1387:1394	proteins from A. thaliana and N. benthamiana	1387:1430	While the proteins identified in O. sativa are associated with diverse functions, proteins from A. thaliana and N. benthamiana are mainly involved in cell wall biosynthesis.					
33777069	11	88	from	presence	1653:1660	arg1	database					1728:1735	the 1001 genome database	1712:1735	the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis	1712:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	11	88	from	presence	1653:1660	arg1	accessions					1696:1705	selected accessions	1687:1705	selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis	1687:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	0	89	dep	Appear	79:84	arg1	Conserved					101:109	Conserved	101:109	Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions	79:155	Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions.					
33777069	3	90	theme	known	474:478	arg1	elongation					492:501	the only known outer-chain elongation	465:501	the only known outer-chain elongation of complex N-glycans	465:522	In plants, the Lewis A trisaccharide constitutes the only known outer-chain elongation of complex N-glycans.					
33777069	0	91	theme	A	6:6	arg1	Glycans					8:14	Lewis A Glycans	0:14	Lewis A Glycans	0:14	Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions.					
33777069	7	92	theme	Lewis	1005:1009	arg1	structures					1013:1022	Lewis A structures	1005:1022	Lewis A structures on complex plant N-glycans	1005:1049	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS.					
33777069	11	93	contain	containing	1737:1746	arg1	database					1728:1735	the 1001 genome database	1712:1735	the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis	1712:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	11	93	contain	containing	1737:1746	arg2	variations					1759:1768	amino acid variations	1748:1768	amino acid variations in the enzymes required for Lewis A biosynthesis	1748:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	11	94	from	database	1728:1735	arg1	accessions					1696:1705	selected accessions	1687:1705	selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis	1687:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	11	94	from	database	1728:1735	arg1	presence					1653:1660	the presence	1649:1660	the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis	1649:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	10	95	theme	thaliana	1526:1533	arg1	line					1515:1518	a Lewis A-deficient mutant line	1488:1518	a Lewis A-deficient mutant line of A. thaliana	1488:1533	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	12	96	theme	other	1921:1925	arg1	accessions					1934:1943	all other tested accessions	1917:1943	all other tested accessions	1917:1943	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	3	97	theme	complex	506:512	arg1	N-glycans					514:522	complex N-glycans	506:522	complex N-glycans	506:522	In plants, the Lewis A trisaccharide constitutes the only known outer-chain elongation of complex N-glycans.					
33777069	8	98	theme	Selected	1153:1160	arg1	proteins					1173:1180	identified proteins	1162:1180	Selected identified proteins	1153:1180	Selected identified proteins were recombinantly expressed and the presence of Lewis A confirmed via immunoblotting and site-specific N-glycan analysis.					
33777069	2	99	from	residues	263:270	arg1	N-glycans					288:296	complex-type N-glycans	275:296	complex-type N-glycans	275:296	Terminal sugar residues on complex-type N-glycans mediate various crucial biological processes in mammals such as cell-cell recognition or protein-ligand interactions.					
33777069	11	100	theme	acid	1754:1757	arg1	variations					1759:1768	amino acid variations	1748:1768	amino acid variations in the enzymes required for Lewis A biosynthesis	1748:1817	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	6	101	theme	A	819:819	arg1	identification					795:808	the identification	791:808	the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa	791:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	12	102	theme	Lewis	1876:1880	arg1	A					1882:1882	Lewis A	1876:1882	Lewis A	1876:1882	Besides one relict line showing no detectable levels of Lewis A, the modification was present in all other tested accessions.					
33777069	3	103	theme	Lewis	431:435	arg1	trisaccharide					439:451	the Lewis A trisaccharide	427:451	the Lewis A trisaccharide	427:451	In plants, the Lewis A trisaccharide constitutes the only known outer-chain elongation of complex N-glycans.					
33777069	1	104	attach	present	216:222	arg1	life					242:245	life	242:245	life	242:245	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	1	104	attach	present	216:222	arg1	domains					231:237	all domains	227:237	all domains of life	227:245	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	1	104	attach	present	216:222	arg2	N-glycosylation					158:172	N-glycosylation	158:172	N-glycosylation	158:172	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	1	104	attach	present	216:222	arg2	modification					203:214	a highly abundant protein modification	177:214	a highly abundant protein modification present in all domains of life	177:245	N-glycosylation is a highly abundant protein modification present in all domains of life.					
33777069	7	105	theme	JIM84	972:976	arg1	antibody					978:985	the JIM84 antibody	968:985	the JIM84 antibody	968:985	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS.					
33777069	9	106	theme	cell	1455:1458	arg1	biosynthesis					1465:1476	cell wall biosynthesis	1455:1476	cell wall biosynthesis	1455:1476	While the proteins identified in O. sativa are associated with diverse functions, proteins from A. thaliana and N. benthamiana are mainly involved in cell wall biosynthesis.					
33777069	13	107	theme	different	2056:2064	arg1	species					2066:2072	different species	2056:2072	different species	2056:2072	The data provided here comprises the so far first attempt at identifying Lewis A bearing glycoproteins across different species and will help to shed more light on the role of Lewis A structures in plants.					
33777069	10	108	theme	wall	1573:1576	arg1	lignin					1612:1617	lignin	1612:1617	lignin	1612:1617	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	10	108	theme	wall	1573:1576	arg1	constituents					1578:1589	cell wall constituents	1568:1589	cell wall constituents such as cellulose or lignin	1568:1617	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	10	108	theme	wall	1573:1576	arg1	cellulose					1599:1607	cellulose	1599:1607	cellulose	1599:1607	However, a Lewis A-deficient mutant line of A. thaliana showed no change in abundance of cell wall constituents such as cellulose or lignin.					
33777069	6	109	theme	Arabidopsis	879:889	arg1	species					870:876	three different plant species	848:876	three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa	848:939	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	6	109	theme	Arabidopsis	879:889	arg1	thaliana					891:898	Arabidopsis thaliana	879:898	Arabidopsis thaliana	879:898	Here, we report the identification of Lewis A bearing glycoproteins from three different plant species: Arabidopsis thaliana, Nicotiana benthamiana, and Oryza sativa.					
33777069	11	110	theme	A	1671:1671	arg1	structures					1673:1682	Lewis A structures	1665:1682	Lewis A structures	1665:1682	Furthermore, we investigated the presence of Lewis A structures in selected accessions from the 1001 genome database containing amino acid variations in the enzymes required for Lewis A biosynthesis.					
33777069	2	111	theme	protein-ligand	387:400	arg1	interactions					402:413	protein-ligand interactions	387:413	protein-ligand interactions	387:413	Terminal sugar residues on complex-type N-glycans mediate various crucial biological processes in mammals such as cell-cell recognition or protein-ligand interactions.					
33777069	7	112	theme	Affinity	942:949	arg1	purification					951:962	Affinity purification	942:962	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans,	942:1050	Affinity purification via the JIM84 antibody, directed against Lewis A structures on complex plant N-glycans, was used to enrich Lewis A bearing glycoproteins, which were subsequently identified via nano-LC-MS.					
32513872	4	0	theme	wasting	995:1001	arg1	disease					1003:1009	chronic wasting disease	987:1009	chronic wasting disease	987:1009	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	6	1	theme	prion	1334:1338	arg1	detection					1340:1348	prion detection	1334:1348	prion detection	1334:1348	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	6	2	theme	resistance	1627:1636	arg1	evaluation					1604:1613	evaluation	1604:1613	evaluation of protease resistance	1604:1636	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	1	3	theme	conformational	173:186	arg1	conversion					188:197	conformational conversion	173:197	conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc)	173:270	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	4	4	theme	PrPSc	1075:1079	arg1	feature					1111:1117	a consistent feature	1098:1117	a consistent feature of prion pathogenesis	1098:1139	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	4	4	theme	PrPSc	1075:1079	arg1	glycosylation					1081:1093	incomplete PrPSc glycosylation	1064:1093	incomplete PrPSc glycosylation	1064:1093	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	5	5	theme	direct	1234:1239	arg1	means					1241:1245	a direct means	1232:1245	a direct means of distinguishing different prion strains	1232:1287	We also show that interrogating the conformational properties of the PRC7 epitope affords a direct means of distinguishing different prion strains.					
32513872	4	6	theme	chronic	987:993	arg1	disease					1003:1009	chronic wasting disease	987:1009	chronic wasting disease	987:1009	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	3	7	theme	prion	672:676	arg1	infection					678:686	prion infection	672:686	prion infection	672:686	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	4	8	theme	PrPSc	862:866	arg1	Detection					835:843	Detection	835:843	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease	835:1009	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	4	8	theme	PrPSc	862:866	arg1	encephalopathy					1034:1047	transmissible mink encephalopathy	1015:1047	transmissible mink encephalopathy	1015:1047	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	1	9	theme	monoclonal	345:354	arg1	mAbs					368:371	mAbs	368:371	mAbs	368:371	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	1	9	theme	monoclonal	345:354	arg1	antibodies					356:365	monoclonal antibodies	345:365	monoclonal antibodies (mAbs) that can distinguish these conformational isoforms	345:423	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	6	10	theme	approach	1321:1328	arg1	specificity					1302:1312	the specificity	1298:1312	the specificity of our approach for prion detection and strain discrimination	1298:1374	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	5	11	theme	epitope	1216:1222	arg1	properties					1193:1202	the conformational properties	1174:1202	the conformational properties of the PRC7 epitope	1174:1222	We also show that interrogating the conformational properties of the PRC7 epitope affords a direct means of distinguishing different prion strains.					
32513872	3	12	theme	mAb	800:802	arg1	recognition					804:814	mAb recognition	800:814	mAb recognition upon renaturation	800:832	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	0	13	theme	prion	97:101	arg1	detection					103:111	prion detection	97:111	prion detection	97:111	Incomplete glycosylation during prion infection unmasks a prion protein epitope that facilitates prion detection and strain discrimination.					
32513872	1	14	theme	antibodies	356:365	arg1	lack					337:340	a lack	335:340	a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms	335:423	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	4	15	theme	experimental	871:882	arg1	infections					896:905	experimental and natural infections	871:905	experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease	871:1009	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	7	16	theme	PrP	1876:1878	arg1	instability					1835:1845	structural instability	1824:1845	structural instability of incompletely glycosylated PrP	1824:1878	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	7	17	theme	strain	1729:1734	arg1	differentiation					1736:1750	strain differentiation	1729:1750	strain differentiation	1729:1750	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	6	18	theme	protease	1618:1625	arg1	resistance					1627:1636	protease resistance	1618:1636	protease resistance	1618:1636	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	1	19	theme	prion	279:283	arg1	infection					285:293	prion infection	279:293	prion infection	279:293	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	0	20	theme	strain	117:122	arg1	discrimination					124:137	strain discrimination	117:137	strain discrimination	117:137	Incomplete glycosylation during prion infection unmasks a prion protein epitope that facilitates prion detection and strain discrimination.					
32513872	3	21	from	Asn-196	731:737	arg1	glycosylation					714:726	diminished PrPSc glycosylation	697:726	diminished PrPSc glycosylation at Asn-196	697:737	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	5	22	theme	distinguishing	1250:1263	arg1	strains					1281:1287	distinguishing different prion strains	1250:1287	distinguishing different prion strains	1250:1287	We also show that interrogating the conformational properties of the PRC7 epitope affords a direct means of distinguishing different prion strains.					
32513872	7	23	theme	replication	1803:1813	arg1	mechanism					1784:1792	a mechanism	1782:1792	a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc	1782:1940	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	4	24	theme	transmissible	1015:1027	arg1	encephalopathy					1034:1047	transmissible mink encephalopathy	1015:1047	transmissible mink encephalopathy	1015:1047	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	3	25	theme	diminished	697:706	arg1	glycosylation					714:726	diminished PrPSc glycosylation	697:726	diminished PrPSc glycosylation at Asn-196	697:737	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	5	26	theme	prion	1275:1279	arg1	strains					1281:1287	distinguishing different prion strains	1250:1287	distinguishing different prion strains	1250:1287	We also show that interrogating the conformational properties of the PRC7 epitope affords a direct means of distinguishing different prion strains.					
32513872	1	27	theme	cellular	202:209	arg1	PrPC					226:229	PrPC	226:229	PrPC	226:229	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	1	27	theme	cellular	202:209	arg1	protein					217:223	cellular prion protein	202:223	cellular prion protein (PrPC)	202:230	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	3	28	theme	PrPSc	708:712	arg1	glycosylation					714:726	diminished PrPSc glycosylation	697:726	diminished PrPSc glycosylation at Asn-196	697:737	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	4	29	theme	PRC7-reactive	848:860	arg1	PrPSc					862:866	PRC7-reactive PrPSc	848:866	PRC7-reactive PrPSc	848:866	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	7	30	theme	structural	1824:1833	arg1	instability					1835:1845	structural instability	1824:1845	structural instability of incompletely glycosylated PrP	1824:1878	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	6	31	theme	strain	1354:1359	arg1	discrimination					1361:1374	strain discrimination	1354:1374	strain discrimination	1354:1374	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	1	32	theme	prion	211:215	arg1	PrPC					226:229	PrPC	226:229	PrPC	226:229	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	1	32	theme	prion	211:215	arg1	protein					217:223	cellular prion protein	202:223	cellular prion protein (PrPC)	202:230	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	0	33	theme	Incomplete	0:9	arg1	glycosylation					11:23	Incomplete glycosylation	0:23	Incomplete glycosylation during prion infection	0:46	Incomplete glycosylation during prion infection unmasks a prion protein epitope that facilitates prion detection and strain discrimination.					
32513872	5	34	theme	PRC7	1211:1214	arg1	epitope					1216:1222	the PRC7 epitope	1207:1222	the PRC7 epitope	1207:1222	We also show that interrogating the conformational properties of the PRC7 epitope affords a direct means of distinguishing different prion strains.					
32513872	4	35	theme	mink	1029:1032	arg1	encephalopathy					1034:1047	transmissible mink encephalopathy	1015:1047	transmissible mink encephalopathy	1015:1047	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	2	36	attach	attached	537:544	arg2	glycans					525:531	glycans	525:531	glycans	525:531	Here we show that the anti-PrP mAb PRC7 recognizes an epitope that is shielded from detection when glycans are attached to Asn-196.					
32513872	2	36	attach	attached	537:544	arg1	Asn-196					549:555	Asn-196	549:555	Asn-196	549:555	Here we show that the anti-PrP mAb PRC7 recognizes an epitope that is shielded from detection when glycans are attached to Asn-196.					
32513872	1	37	theme	protein	217:223	arg1	conversion					188:197	conformational conversion	173:197	conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc)	173:270	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	0	38	theme	prion	32:36	arg1	infection					38:46	prion infection	32:46	prion infection	32:46	Incomplete glycosylation during prion infection unmasks a prion protein epitope that facilitates prion detection and strain discrimination.					
32513872	3	39	gly	glycosylation	714:726	arg2	Asn-196					731:737	Asn-196	731:737	Asn-196	731:737	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	3	39	gly	glycosylation	714:726	arg1	Asn-196					731:737	Asn-196	731:737	Asn-196	731:737	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	4	40	theme	scrapie	934:940	arg1	strains					942:948	various mouse-adapted scrapie strains	912:948	various mouse-adapted scrapie strains	912:948	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	4	41	dep	deer	974:977	arg1	disease					1003:1009	chronic wasting disease	987:1009	chronic wasting disease	987:1009	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	4	42	theme	pathogenesis	1128:1139	arg1	feature					1111:1117	a consistent feature	1098:1117	a consistent feature of prion pathogenesis	1098:1139	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	4	42	theme	pathogenesis	1128:1139	arg1	glycosylation					1081:1093	incomplete PrPSc glycosylation	1064:1093	incomplete PrPSc glycosylation	1064:1093	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	4	43	theme	mouse-adapted	920:932	arg1	strains					942:948	various mouse-adapted scrapie strains	912:948	various mouse-adapted scrapie strains	912:948	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	7	44	theme	glycosylated	1863:1874	arg1	PrP					1876:1878	incompletely glycosylated PrP	1850:1878	incompletely glycosylated PrP	1850:1878	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	6	45	from	PrPC	1588:1591	arg1	PrPSc					1577:1581	PrPSc	1577:1581	PrPSc from PrPC	1577:1591	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	4	46	with	infections	896:905	arg1	strains					942:948	various mouse-adapted scrapie strains	912:948	various mouse-adapted scrapie strains	912:948	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	4	46	with	infections	896:905	arg1	prions					959:964	prions	959:964	prions causing deer and elk chronic wasting disease	959:1009	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	6	47	link	N-linked	1406:1413	arg1	glycosylation					1415:1427	N-linked glycosylation	1406:1427	N-linked glycosylation	1406:1427	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	4	48	from	encephalopathy	1034:1047	arg1	infections					896:905	experimental and natural infections	871:905	experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease	871:1009	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	6	49	theme	antibody	1468:1475	arg1	recognition					1477:1487	antibody recognition	1468:1487	antibody recognition	1468:1487	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	3	50	theme	PRC7	767:770	arg1	amenable					788:795	amenable	788:795	amenable	788:795	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	3	50	theme	PRC7	767:770	arg1	epitope					772:778	an unshielded PRC7 epitope	753:778	an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation	753:832	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	4	51	theme	incomplete	1064:1073	arg1	feature					1111:1117	a consistent feature	1098:1117	a consistent feature of prion pathogenesis	1098:1139	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	4	51	theme	incomplete	1064:1073	arg1	glycosylation					1081:1093	incomplete PrPSc glycosylation	1064:1093	incomplete PrPSc glycosylation	1064:1093	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	2	52	theme	mAb	457:459	arg1	PRC7					461:464	the anti-PrP mAb PRC7	444:464	the anti-PrP mAb PRC7	444:464	Here we show that the anti-PrP mAb PRC7 recognizes an epitope that is shielded from detection when glycans are attached to Asn-196.					
32513872	5	53	theme	conformational	1178:1191	arg1	properties					1193:1202	the conformational properties	1174:1202	the conformational properties of the PRC7 epitope	1174:1222	We also show that interrogating the conformational properties of the PRC7 epitope affords a direct means of distinguishing different prion strains.					
32513872	0	54	theme	protein	64:70	arg1	epitope					72:78	a prion protein epitope	56:78	a prion protein epitope that facilitates prion detection and strain discrimination	56:137	Incomplete glycosylation during prion infection unmasks a prion protein epitope that facilitates prion detection and strain discrimination.					
32513872	4	55	from	Detection	835:843	arg1	infections					896:905	experimental and natural infections	871:905	experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease	871:1009	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	2	56	theme	anti-PrP	448:455	arg1	PRC7					461:464	the anti-PrP mAb PRC7	444:464	the anti-PrP mAb PRC7	444:464	Here we show that the anti-PrP mAb PRC7 recognizes an epitope that is shielded from detection when glycans are attached to Asn-196.					
32513872	7	57	theme	innovative	1674:1683	arg1	strategy					1696:1703	an innovative and facile strategy	1671:1703	an innovative and facile strategy for prion detection and strain differentiation	1671:1750	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	4	58	theme	natural	888:894	arg1	infections					896:905	experimental and natural infections	871:905	experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease	871:1009	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	1	59	theme	conformational	401:414	arg1	isoforms					416:423	these conformational isoforms	395:423	these conformational isoforms	395:423	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	3	60	theme	unshielded	756:765	arg1	amenable					788:795	amenable	788:795	amenable	788:795	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	3	60	theme	unshielded	756:765	arg1	epitope					772:778	an unshielded PRC7 epitope	753:778	an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation	753:832	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	4	61	theme	various	912:918	arg1	strains					942:948	various mouse-adapted scrapie strains	912:948	various mouse-adapted scrapie strains	912:948	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	3	62	theme	full	606:609	arg1	glycosylation					611:623	full glycosylation	606:623	full glycosylation	606:623	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	7	63	theme	prion	1797:1801	arg1	replication					1803:1813	prion replication	1797:1813	prion replication	1797:1813	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	4	64	theme	consistent	1100:1109	arg1	feature					1111:1117	a consistent feature	1098:1117	a consistent feature of prion pathogenesis	1098:1139	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	4	64	theme	consistent	1100:1109	arg1	glycosylation					1081:1093	incomplete PrPSc glycosylation	1064:1093	incomplete PrPSc glycosylation	1064:1093	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	5	65	theme	different	1265:1273	arg1	strains					1281:1287	distinguishing different prion strains	1250:1287	distinguishing different prion strains	1250:1287	We also show that interrogating the conformational properties of the PRC7 epitope affords a direct means of distinguishing different prion strains.					
32513872	7	66	with	consistent	1766:1775	arg1	mechanism					1784:1792	a mechanism	1782:1792	a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc	1782:1940	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	7	67	theme	prion	1709:1713	arg1	detection					1715:1723	prion detection	1709:1723	prion detection	1709:1723	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	1	68	theme	causative	144:152	arg1	factors					154:160	The causative factors	140:160	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection	140:293	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	4	69	theme	prion	1122:1126	arg1	pathogenesis					1128:1139	prion pathogenesis	1122:1139	prion pathogenesis	1122:1139	Detection of PRC7-reactive PrPSc in experimental and natural infections with various mouse-adapted scrapie strains and with prions causing deer and elk chronic wasting disease and transmissible mink encephalopathy uncovered that incomplete PrPSc glycosylation is a consistent feature of prion pathogenesis.					
32513872	1	70	theme	infectious	241:250	arg1	counterpart					252:262	its infectious counterpart	237:262	its infectious counterpart (PrPSc)	237:270	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	1	70	theme	infectious	241:250	arg1	PrPSc					265:269	PrPSc	265:269	PrPSc	265:269	The causative factors underlying conformational conversion of cellular prion protein (PrPC) into its infectious counterpart (PrPSc) during prion infection remain undetermined, in part because of a lack of monoclonal antibodies (mAbs) that can distinguish these conformational isoforms.					
32513872	6	71	theme	PrP	1450:1452	arg1	epitopes					1454:1461	PrP epitopes	1450:1461	PrP epitopes	1450:1461	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	3	72	theme	PRC7	656:659	arg1	detection					661:669	PRC7 detection	656:669	PRC7 detection	656:669	We observed that whereas PrPC is predisposed to full glycosylation and is therefore refractory to PRC7 detection, prion infection leads to diminished PrPSc glycosylation at Asn-196, resulting in an unshielded PRC7 epitope that is amenable to mAb recognition upon renaturation.					
32513872	7	73	theme	PrPC	1928:1931	arg1	conversion					1914:1923	the conformational conversion	1895:1923	the conformational conversion of PrPC to PrPSc	1895:1940	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	6	74	theme	standard	1539:1546	arg1	manipulations					1548:1560	additional standard manipulations	1528:1560	additional standard manipulations	1528:1560	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	5	75	theme	strains	1281:1287	arg1	means					1241:1245	a direct means	1232:1245	a direct means of distinguishing different prion strains	1232:1287	We also show that interrogating the conformational properties of the PRC7 epitope affords a direct means of distinguishing different prion strains.					
32513872	0	76	theme	prion	58:62	arg1	epitope					72:78	a prion protein epitope	56:78	a prion protein epitope that facilitates prion detection and strain discrimination	56:137	Incomplete glycosylation during prion infection unmasks a prion protein epitope that facilitates prion detection and strain discrimination.					
32513872	7	77	theme	facile	1689:1694	arg1	strategy					1696:1703	an innovative and facile strategy	1671:1703	an innovative and facile strategy for prion detection and strain differentiation	1671:1750	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	7	78	theme	conformational	1899:1912	arg1	conversion					1914:1923	the conformational conversion	1895:1923	the conformational conversion of PrPC to PrPSc	1895:1940	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	7	79	gly	glycosylated	1863:1874	arg1	PrP					1876:1878	incompletely glycosylated PrP	1850:1878	incompletely glycosylated PrP	1850:1878	Our findings not only highlight an innovative and facile strategy for prion detection and strain differentiation, but are also consistent with a mechanism of prion replication in which structural instability of incompletely glycosylated PrP contributes to the conformational conversion of PrPC to PrPSc.					
32513872	6	80	theme	additional	1528:1537	arg1	manipulations					1548:1560	additional standard manipulations	1528:1560	additional standard manipulations	1528:1560	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
32513872	6	81	theme	N-linked	1406:1413	arg1	glycosylation					1415:1427	N-linked glycosylation	1406:1427	N-linked glycosylation	1406:1427	Because the specificity of our approach for prion detection and strain discrimination relies on the extent to which N-linked glycosylation shields or unshields PrP epitopes from antibody recognition, it dispenses with the requirement for additional standard manipulations to distinguish PrPSc from PrPC, including evaluation of protease resistance.					
33657316	0	0	theme	Charge	84:89	arg1	Spectrometry					106:117	Charge Detection Mass Spectrometry	84:117	Charge Detection Mass Spectrometry	84:117	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.					
33657316	5	1	theme	glycan	1118:1123	arg1	processing					1125:1134	glycan processing	1118:1134	glycan processing on each trimer	1118:1149	Moreover, the distribution of glycan masses determined by CDMS is much broader than the distribution expected from the glycoproteomics studies, assuming that glycan processing on each trimer is not correlated.					
33657316	1	2	from	sites	187:191	arg1	trimer					208:213	the protein trimer	196:213	the protein trimer making up the spike (S) region of the SARS-CoV-2 virus	196:268	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	7	3	theme	system	1460:1465	arg1	way					1415:1417	a way	1413:1417	a way of further confounding the host's immune system	1413:1465	This heterogeneity may have evolved as a way of further confounding the host's immune system.					
33657316	4	4	theme	glycoproteomics	824:838	arg1	studies					840:846	the "bottom-up" glycoproteomics studies	808:846	the "bottom-up" glycoproteomics studies	808:846	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	0	5	theme	Mass	101:104	arg1	Spectrometry					106:117	Charge Detection Mass Spectrometry	84:117	Charge Detection Mass Spectrometry	84:117	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.					
33657316	0	6	from	Heterogeneity	0:12	arg1	Protein					64:70	Trimeric SARS-CoV-2 Spike Protein	38:70	Trimeric SARS-CoV-2 Spike Protein	38:70	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.					
33657316	6	7	theme	processing	1272:1281	arg1	extent					1255:1260	the extent	1251:1260	the extent of glycan processing of the S protein trimers	1251:1306	The breadth of the glycan mass distribution therefore indicates heterogeneity in the extent of glycan processing of the S protein trimers, with some trimers being much more heavily processed than others.					
33657316	4	8	theme	"	745:745	arg1	measurements					752:763	"top-down" CDMS measurements	736:763	"top-down" CDMS measurements	736:763	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	0	9	theme	Detection	91:99	arg1	Spectrometry					106:117	Charge Detection Mass Spectrometry	84:117	Charge Detection Mass Spectrometry	84:117	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.					
33657316	5	10	theme	glycoproteomics	1079:1093	arg1	studies					1095:1101	the glycoproteomics studies	1075:1101	the glycoproteomics studies	1075:1101	Moreover, the distribution of glycan masses determined by CDMS is much broader than the distribution expected from the glycoproteomics studies, assuming that glycan processing on each trimer is not correlated.					
33657316	6	11	theme	glycan	1265:1270	arg1	processing					1272:1281	glycan processing	1265:1281	glycan processing of the S protein trimers	1265:1306	The breadth of the glycan mass distribution therefore indicates heterogeneity in the extent of glycan processing of the S protein trimers, with some trimers being much more heavily processed than others.					
33657316	1	12	from	glycosylation	154:166	arg1	trimer					208:213	the protein trimer	196:213	the protein trimer making up the spike (S) region of the SARS-CoV-2 virus	196:268	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	4	13	theme	top-down	737:744	arg1	measurements					752:763	"top-down" CDMS measurements	736:763	"top-down" CDMS measurements	736:763	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	2	14	theme	heterogeneous	486:498	arg1	trimer					552:557	the heavily glycosylated S protein trimer	517:557	the heavily glycosylated S protein trimer	517:557	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	2	14	theme	heterogeneous	486:498	arg1	proteins					500:507	highly heterogeneous proteins	479:507	highly heterogeneous proteins such as the heavily glycosylated S protein trimer	479:557	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	6	15	from	heterogeneity	1234:1246	arg1	extent					1255:1260	the extent	1251:1260	the extent of glycan processing of the S protein trimers	1251:1306	The breadth of the glycan mass distribution therefore indicates heterogeneity in the extent of glycan processing of the S protein trimers, with some trimers being much more heavily processed than others.					
33657316	1	16	theme	N-glycan	178:185	arg1	sites					187:191	the 66 N-glycan sites	171:191	the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus	171:268	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	7	17	theme	further	1422:1428	arg1	system					1460:1465	further confounding the host's immune system	1422:1465	further confounding the host's immune system	1422:1465	This heterogeneity may have evolved as a way of further confounding the host's immune system.					
33657316	5	18	from	processing	1125:1134	arg1	trimer					1144:1149	each trimer	1139:1149	each trimer	1139:1149	Moreover, the distribution of glycan masses determined by CDMS is much broader than the distribution expected from the glycoproteomics studies, assuming that glycan processing on each trimer is not correlated.					
33657316	4	19	theme	CDMS	747:750	arg1	measurements					752:763	"top-down" CDMS measurements	736:763	"top-down" CDMS measurements	736:763	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	7	20	theme	confounding	1430:1440	arg1	system					1460:1465	further confounding the host's immune system	1422:1465	further confounding the host's immune system	1422:1465	This heterogeneity may have evolved as a way of further confounding the host's immune system.					
33657316	1	21	theme	sites	187:191	arg1	glycosylation					154:166	glycosylation	154:166	glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus	154:268	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	4	22	theme	glycoproteomic	869:882	arg1	measurements					884:895	the glycoproteomic measurements	865:895	the glycoproteomic measurements	865:895	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	4	23	theme	"	822:822	arg1	studies					840:846	the "bottom-up" glycoproteomics studies	808:846	the "bottom-up" glycoproteomics studies	808:846	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	6	24	theme	S	1290:1290	arg1	trimers					1300:1306	the S protein trimers	1286:1306	the S protein trimers	1286:1306	The breadth of the glycan mass distribution therefore indicates heterogeneity in the extent of glycan processing of the S protein trimers, with some trimers being much more heavily processed than others.					
33657316	6	25	theme	trimers	1300:1306	arg1	processing					1272:1281	glycan processing	1265:1281	glycan processing of the S protein trimers	1265:1306	The breadth of the glycan mass distribution therefore indicates heterogeneity in the extent of glycan processing of the S protein trimers, with some trimers being much more heavily processed than others.					
33657316	3	26	theme	specific	669:676	arg1	sites					678:682	specific sites	669:682	specific sites	669:682	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	2	27	theme	ions	424:427	arg1	ratio					393:397	the mass-to-charge ratio	374:397	the mass-to-charge ratio	374:397	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	2	27	theme	ions	424:427	arg1	charge					403:408	charge	403:408	charge	403:408	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	1	28	theme	SARS-CoV-2	253:262	arg1	virus					264:268	the SARS-CoV-2 virus	249:268	the SARS-CoV-2 virus	249:268	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	2	29	theme	simultaneous	346:357	arg1	measurement					359:369	simultaneous measurement	346:369	simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer	346:557	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	0	30	theme	Processing	24:33	arg1	Heterogeneity					0:12	Heterogeneity	0:12	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein	0:70	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.					
33657316	1	31	theme	virus	264:268	arg1	region					239:244	the spike (S) region	225:244	the spike (S) region of the SARS-CoV-2 virus	225:268	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	2	32	theme	glycosylated	529:540	arg1	trimer					552:557	the heavily glycosylated S protein trimer	517:557	the heavily glycosylated S protein trimer	517:557	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	6	33	theme	distribution	1201:1212	arg1	breadth					1174:1180	The breadth	1170:1180	The breadth of the glycan mass distribution	1170:1212	The breadth of the glycan mass distribution therefore indicates heterogeneity in the extent of glycan processing of the S protein trimers, with some trimers being much more heavily processed than others.					
33657316	1	34	theme	protein	200:206	arg1	trimer					208:213	the protein trimer	196:213	the protein trimer making up the spike (S) region of the SARS-CoV-2 virus	196:268	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	0	35	theme	Glycan	17:22	arg1	Processing					24:33	Glycan Processing	17:33	Glycan Processing	17:33	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.					
33657316	0	36	theme	Trimeric	38:45	arg1	Protein					64:70	Trimeric SARS-CoV-2 Spike Protein	38:70	Trimeric SARS-CoV-2 Spike Protein	38:70	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.					
33657316	4	37	theme	larger	930:935	arg1	glycans					951:957	larger, more-complex glycans	930:957	larger, more-complex glycans	930:957	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	3	38	theme	structure	631:639	arg1	studies					616:622	recent glycoproteomics studies	593:622	recent glycoproteomics studies of the structure and abundance of glycans at specific sites	593:682	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	0	39	gly	Heterogeneity	0:12	arg1	Processing					24:33	Glycan Processing	17:33	Glycan Processing	17:33	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.					
33657316	2	40	theme	ratio	393:397	arg1	measurement					359:369	simultaneous measurement	346:369	simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer	346:557	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	2	41	theme	mass	438:441	arg1	distributions					443:455	mass distributions	438:455	mass distributions	438:455	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	4	42	dep	larger	930:935	arg1	more-complex					938:949	more-complex	938:949	more-complex	938:949	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	2	43	theme	individual	413:422	arg1	ions					424:427	individual ions	413:427	individual ions	413:427	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	2	44	theme	mass-to-charge	378:391	arg1	ratio					393:397	the mass-to-charge ratio	374:397	the mass-to-charge ratio	374:397	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	0	45	theme	Spike	58:62	arg1	Protein					64:70	Trimeric SARS-CoV-2 Spike Protein	38:70	Trimeric SARS-CoV-2 Spike Protein	38:70	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.					
33657316	3	46	from	studies	616:622	arg1	sites					678:682	specific sites	669:682	specific sites	669:682	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	1	47	dep	spike	229:233	arg1	S					236:236	S	236:236	S	236:236	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	6	48	theme	protein	1292:1298	arg1	trimers					1300:1306	the S protein trimers	1286:1306	the S protein trimers	1286:1306	The breadth of the glycan mass distribution therefore indicates heterogeneity in the extent of glycan processing of the S protein trimers, with some trimers being much more heavily processed than others.					
33657316	0	49	theme	SARS-CoV-2	47:56	arg1	Protein					64:70	Trimeric SARS-CoV-2 Spike Protein	38:70	Trimeric SARS-CoV-2 Spike Protein	38:70	Heterogeneity of Glycan Processing on Trimeric SARS-CoV-2 Spike Protein Revealed by Charge Detection Mass Spectrometry.					
33657316	1	50	theme	charge	291:296	arg1	CDMS					327:330	CDMS	327:330	CDMS	327:330	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	1	50	theme	charge	291:296	arg1	spectrometry					313:324	charge detection mass spectrometry	291:324	charge detection mass spectrometry (CDMS)	291:331	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	1	51	gly	glycosylation	154:166	arg1	sites					187:191	the 66 N-glycan sites	171:191	the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus	171:268	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	3	52	theme	CDMS	564:567	arg1	results					569:575	The CDMS results	560:575	The CDMS results	560:575	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	4	53	theme	glycans	951:957	arg1	abundances					916:925	the abundances	912:925	the abundances of larger, more-complex glycans	912:957	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	1	54	theme	detection	298:306	arg1	CDMS					327:330	CDMS	327:330	CDMS	327:330	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	1	54	theme	detection	298:306	arg1	spectrometry					313:324	charge detection mass spectrometry	291:324	charge detection mass spectrometry (CDMS)	291:331	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	2	55	theme	protein	544:550	arg1	trimer					552:557	the heavily glycosylated S protein trimer	517:557	the heavily glycosylated S protein trimer	517:557	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	3	56	theme	glycans	658:664	arg1	abundance					645:653	abundance	645:653	abundance	645:653	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	3	56	theme	glycans	658:664	arg1	structure					631:639	structure	631:639	structure	631:639	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	3	57	theme	recent	593:598	arg1	studies					616:622	recent glycoproteomics studies	593:622	recent glycoproteomics studies of the structure and abundance of glycans at specific sites	593:682	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	1	58	from	trimer	208:213	arg1	glycosylation					154:166	glycosylation	154:166	glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus	154:268	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	4	59	theme	glycan	708:713	arg1	masses					715:720	average glycan masses	700:720	average glycan masses determined by "top-down" CDMS measurements	700:763	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	2	60	theme	S	542:542	arg1	trimer					552:557	the heavily glycosylated S protein trimer	517:557	the heavily glycosylated S protein trimer	517:557	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	6	61	theme	mass	1196:1199	arg1	distribution					1201:1212	the glycan mass distribution	1185:1212	the glycan mass distribution	1185:1212	The breadth of the glycan mass distribution therefore indicates heterogeneity in the extent of glycan processing of the S protein trimers, with some trimers being much more heavily processed than others.					
33657316	3	62	theme	glycoproteomics	600:614	arg1	studies					616:622	recent glycoproteomics studies	593:622	recent glycoproteomics studies of the structure and abundance of glycans at specific sites	593:682	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	4	63	theme	average	700:706	arg1	masses					715:720	average glycan masses	700:720	average glycan masses determined by "top-down" CDMS measurements	700:763	Interestingly, average glycan masses determined by "top-down" CDMS measurements are 35-47% larger than those obtained from the "bottom-up" glycoproteomics studies, suggesting that the glycoproteomic measurements underestimated the abundances of larger, more-complex glycans.					
33657316	6	64	theme	glycan	1189:1194	arg1	distribution					1201:1212	the glycan mass distribution	1185:1212	the glycan mass distribution	1185:1212	The breadth of the glycan mass distribution therefore indicates heterogeneity in the extent of glycan processing of the S protein trimers, with some trimers being much more heavily processed than others.					
33657316	1	65	theme	spike	229:233	arg1	region					239:244	the spike (S) region	225:244	the spike (S) region of the SARS-CoV-2 virus	225:268	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	3	66	theme	abundance	645:653	arg1	studies					616:622	recent glycoproteomics studies	593:622	recent glycoproteomics studies of the structure and abundance of glycans at specific sites	593:682	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	5	67	theme	glycan	990:995	arg1	masses					997:1002	glycan masses	990:1002	glycan masses determined by CDMS	990:1021	Moreover, the distribution of glycan masses determined by CDMS is much broader than the distribution expected from the glycoproteomics studies, assuming that glycan processing on each trimer is not correlated.					
33657316	2	68	theme	charge	403:408	arg1	measurement					359:369	simultaneous measurement	346:369	simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer	346:557	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	7	69	theme	immune	1453:1458	arg1	system					1460:1465	further confounding the host's immune system	1422:1465	further confounding the host's immune system	1422:1465	This heterogeneity may have evolved as a way of further confounding the host's immune system.					
33657316	3	70	from	sites	678:682	arg1	abundance					645:653	abundance	645:653	abundance	645:653	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	3	70	from	sites	678:682	arg1	studies					616:622	recent glycoproteomics studies	593:622	recent glycoproteomics studies of the structure and abundance of glycans at specific sites	593:682	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	3	70	from	sites	678:682	arg1	structure					631:639	structure	631:639	structure	631:639	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
33657316	1	71	theme	mass	308:311	arg1	CDMS					327:330	CDMS	327:330	CDMS	327:330	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	1	71	theme	mass	308:311	arg1	spectrometry					313:324	charge detection mass spectrometry	291:324	charge detection mass spectrometry (CDMS)	291:331	The heterogeneity associated with glycosylation of the 66 N-glycan sites on the protein trimer making up the spike (S) region of the SARS-CoV-2 virus has been assessed by charge detection mass spectrometry (CDMS).					
33657316	5	72	theme	masses	997:1002	arg1	distribution					974:985	the distribution	970:985	the distribution of glycan masses determined by CDMS	970:1021	Moreover, the distribution of glycan masses determined by CDMS is much broader than the distribution expected from the glycoproteomics studies, assuming that glycan processing on each trimer is not correlated.					
33657316	5	72	theme	masses	997:1002	arg1	broader					1031:1037	broader	1031:1037	broader	1031:1037	Moreover, the distribution of glycan masses determined by CDMS is much broader than the distribution expected from the glycoproteomics studies, assuming that glycan processing on each trimer is not correlated.					
33657316	2	73	gly	glycosylated	529:540	arg1	trimer					552:557	the heavily glycosylated S protein trimer	517:557	the heavily glycosylated S protein trimer	517:557	CDMS allows simultaneous measurement of the mass-to-charge ratio and charge of individual ions, so that mass distributions can be determined for highly heterogeneous proteins such as the heavily glycosylated S protein trimer.					
33657316	3	74	dep	structure	631:639	arg1	the					627:629	the	627:629	the	627:629	The CDMS results are compared to recent glycoproteomics studies of the structure and abundance of glycans at specific sites.					
34489044	4	0	theme	model	528:532	arg1	analysis					494:501	The analysis	490:501	The analysis of structural homologous model	490:532	The analysis of structural homologous model revealed that five residues, Arg116, Asn231, His305, Tyr307, and Trp370, constituted the active site of ManAC.					
34489044	7	1	theme	bean	1077:1080	arg1	U/mg					1093:1096	669.7 U/mg	1087:1096	669.7 U/mg	1087:1096	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	7	1	theme	bean	1077:1080	arg1	gum					1082:1084	locust bean gum	1070:1084	locust bean gum (669.7 U/mg)	1070:1097	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	6	2	theme	metal	959:963	arg1	ions					965:968	various metal ions	951:968	various metal ions	951:968	The recombinant ManAC exhibited maximal activity at pH 2.5 and 70 °C, and it was acid tolerant at a pH range of 2.0-6.0 and thermostable under 60 °C. Meanwhile, the activity of ManAC was not significantly affected by various metal ions, except for Mg2+ and Ag2+.					
34489044	0	3	theme	β-mannanase	84:94	arg1	characterization					27:42	Biochemical and structural characterization	0:42	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.	0:124	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.					
34489044	9	4	contain	have	1437:1440	arg1	mannooligosaccharides					1409:1429	mannooligosaccharides	1409:1429	mannooligosaccharides which have potential for food and feed industrial applications	1409:1492	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	9	4	contain	have	1437:1440	arg2	potential					1442:1450	potential	1442:1450	potential for food and feed industrial applications	1442:1492	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	9	5	theme	mannooligosaccharides	1409:1429	arg1	synthesis					1396:1404	the synthesis	1392:1404	the synthesis of mannooligosaccharides which have potential for food and feed industrial applications	1392:1492	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	9	5	theme	mannooligosaccharides	1409:1429	arg1	mannanse					1292:1299	the first reported mannanse	1273:1299	the first reported mannanse from Aspergillus calidoustus (ManAC)	1273:1336	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	1	6	theme	mannan	193:198	arg1	oligosaccharides					200:215	mannan oligosaccharides	193:215	mannan oligosaccharides	193:215	β-Mannanases hydrolyze lignocellulosic biomass with the release of mannan oligosaccharides, which are considered as renewable resource in higher plants.					
34489044	6	7	theme	various	951:957	arg1	ions					965:968	various metal ions	951:968	various metal ions	951:968	The recombinant ManAC exhibited maximal activity at pH 2.5 and 70 °C, and it was acid tolerant at a pH range of 2.0-6.0 and thermostable under 60 °C. Meanwhile, the activity of ManAC was not significantly affected by various metal ions, except for Mg2+ and Ag2+.					
34489044	3	8	theme	Homology	387:394	arg1	analysis					406:413	Homology alignment analysis	387:413	Homology alignment analysis	387:413	Homology alignment analysis indicated that ManAC belonged to glycosyl hydrolase (GH) 5 family members.					
34489044	6	9	theme	thermostable	858:869	arg1	range					837:841	a pH range	832:841	a pH range of 2.0-6.0 and thermostable	832:869	The recombinant ManAC exhibited maximal activity at pH 2.5 and 70 °C, and it was acid tolerant at a pH range of 2.0-6.0 and thermostable under 60 °C. Meanwhile, the activity of ManAC was not significantly affected by various metal ions, except for Mg2+ and Ag2+.					
34489044	9	10	from	calidoustus	1318:1328	arg1	mannanse					1292:1299	the first reported mannanse	1273:1299	the first reported mannanse from Aspergillus calidoustus (ManAC)	1273:1336	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	9	10	from	calidoustus	1318:1328	arg1	synthesis					1396:1404	the synthesis	1392:1404	the synthesis of mannooligosaccharides which have potential for food and feed industrial applications	1392:1492	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	3	11	theme	alignment	396:404	arg1	analysis					406:413	Homology alignment analysis	387:413	Homology alignment analysis	387:413	Homology alignment analysis indicated that ManAC belonged to glycosyl hydrolase (GH) 5 family members.					
34489044	7	12	theme	locust	1070:1075	arg1	U/mg					1093:1096	669.7 U/mg	1087:1096	669.7 U/mg	1087:1096	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	7	12	theme	locust	1070:1075	arg1	gum					1082:1084	locust bean gum	1070:1084	locust bean gum (669.7 U/mg)	1070:1097	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	9	13	theme	industrial	1470:1479	arg1	applications					1481:1492	industrial applications	1470:1492	industrial applications	1470:1492	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	5	14	theme	ManAC	727:731	arg1	ManAC					727:731	ManAC	727:731	ManAC	727:731	Glu232 and Glu340, proton donor and nucleophile, formed the catalytic residues of ManAC.					
34489044	5	14	theme	ManAC	727:731	arg1	residues					715:722	the catalytic residues	701:722	the catalytic residues of ManAC	701:731	Glu232 and Glu340, proton donor and nucleophile, formed the catalytic residues of ManAC.					
34489044	6	15	theme	2.0-6.0	846:852	arg1	range					837:841	a pH range	832:841	a pH range of 2.0-6.0 and thermostable	832:869	The recombinant ManAC exhibited maximal activity at pH 2.5 and 70 °C, and it was acid tolerant at a pH range of 2.0-6.0 and thermostable under 60 °C. Meanwhile, the activity of ManAC was not significantly affected by various metal ions, except for Mg2+ and Ag2+.					
34489044	7	16	theme	β-mannanase	1041:1051	arg1	activity					1053:1060	the highest β-mannanase activity	1029:1060	the highest β-mannanase activity towards locust bean gum (669.7 U/mg)	1029:1097	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	9	17	dep	food	1456:1459	arg1	applications					1481:1492	industrial applications	1470:1492	industrial applications	1470:1492	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	1	18	theme	oligosaccharides	200:215	arg1	release					182:188	the release	178:188	the release	178:188	β-Mannanases hydrolyze lignocellulosic biomass with the release of mannan oligosaccharides, which are considered as renewable resource in higher plants.					
34489044	1	18	theme	oligosaccharides	200:215	arg1	resource					252:259	renewable resource	242:259	renewable resource in higher plants	242:276	β-Mannanases hydrolyze lignocellulosic biomass with the release of mannan oligosaccharides, which are considered as renewable resource in higher plants.					
34489044	4	19	theme	homologous	517:526	arg1	model					528:532	structural homologous model	506:532	structural homologous model	506:532	The analysis of structural homologous model revealed that five residues, Arg116, Asn231, His305, Tyr307, and Trp370, constituted the active site of ManAC.					
34489044	7	20	theme	Vmax	1115:1118	arg1	values					1120:1125	the Km and Vmax values	1104:1125	the Km and Vmax values	1104:1125	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	7	21	theme	Km	1108:1109	arg1	values					1120:1125	the Km and Vmax values	1104:1125	the Km and Vmax values	1104:1125	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	4	22	theme	structural	506:515	arg1	model					528:532	structural homologous model	506:532	structural homologous model	506:532	The analysis of structural homologous model revealed that five residues, Arg116, Asn231, His305, Tyr307, and Trp370, constituted the active site of ManAC.					
34489044	7	23	theme	982.4 μmol/min/mg	1144:1160	arg1	values					1120:1125	the Km and Vmax values	1104:1125	the Km and Vmax values	1104:1125	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	0	24	theme	Biochemical	0:10	arg1	characterization					27:42	Biochemical and structural characterization	0:42	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.	0:124	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.					
34489044	9	25	theme	reported	1283:1290	arg1	mannanse					1292:1299	the first reported mannanse	1273:1299	the first reported mannanse from Aspergillus calidoustus (ManAC)	1273:1336	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	9	25	theme	reported	1283:1290	arg1	synthesis					1396:1404	the synthesis	1392:1404	the synthesis of mannooligosaccharides which have potential for food and feed industrial applications	1392:1492	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	7	26	theme	highest	1033:1039	arg1	activity					1053:1060	the highest β-mannanase activity	1029:1060	the highest β-mannanase activity towards locust bean gum (669.7 U/mg)	1029:1097	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	3	27	theme	GH	468:469	arg1	members					481:487	glycosyl hydrolase (GH) 5 family members	448:487	glycosyl hydrolase (GH) 5 family members	448:487	Homology alignment analysis indicated that ManAC belonged to glycosyl hydrolase (GH) 5 family members.					
34489044	7	28	theme	3.4 mg/mL	1130:1138	arg1	values					1120:1125	the Km and Vmax values	1104:1125	the Km and Vmax values	1104:1125	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	6	29	theme	maximal	766:772	arg1	activity					774:781	maximal activity	766:781	maximal activity	766:781	The recombinant ManAC exhibited maximal activity at pH 2.5 and 70 °C, and it was acid tolerant at a pH range of 2.0-6.0 and thermostable under 60 °C. Meanwhile, the activity of ManAC was not significantly affected by various metal ions, except for Mg2+ and Ag2+.					
34489044	2	30	theme	novel	326:330	arg1	ManAC					350:354	ManAC	350:354	ManAC	350:354	Here, we cloned, expressed and characterized a novel endo-β-mannanase (ManAC) from Aspergillus calidoustus.					
34489044	2	30	theme	novel	326:330	arg1	endo-β-mannanase					332:347	a novel endo-β-mannanase	324:347	a novel endo-β-mannanase (ManAC)	324:355	Here, we cloned, expressed and characterized a novel endo-β-mannanase (ManAC) from Aspergillus calidoustus.					
34489044	0	31	theme	structural	16:25	arg1	characterization					27:42	Biochemical and structural characterization	0:42	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.	0:124	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.					
34489044	9	32	theme	ManAC	1369:1373	arg1	properties					1355:1364	these excellent properties	1339:1364	these excellent properties of ManAC	1339:1373	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	6	33	theme	pH	834:835	arg1	range					837:841	a pH range	832:841	a pH range of 2.0-6.0 and thermostable	832:869	The recombinant ManAC exhibited maximal activity at pH 2.5 and 70 °C, and it was acid tolerant at a pH range of 2.0-6.0 and thermostable under 60 °C. Meanwhile, the activity of ManAC was not significantly affected by various metal ions, except for Mg2+ and Ag2+.					
34489044	6	34	theme	60 °C.	877:882	arg1	Meanwhile					884:892	60 °C. Meanwhile	877:892	60 °C. Meanwhile	877:892	The recombinant ManAC exhibited maximal activity at pH 2.5 and 70 °C, and it was acid tolerant at a pH range of 2.0-6.0 and thermostable under 60 °C. Meanwhile, the activity of ManAC was not significantly affected by various metal ions, except for Mg2+ and Ag2+.					
34489044	8	35	theme	acidophilicc	1200:1211	arg1	characteristics					1213:1227	These thermophilic and acidophilicc characteristics	1177:1227	These thermophilic and acidophilicc characteristics	1177:1227	These thermophilic and acidophilicc characteristics is better than most extreme β-mannanase.					
34489044	3	36	theme	glycosyl	448:455	arg1	members					481:487	glycosyl hydrolase (GH) 5 family members	448:487	glycosyl hydrolase (GH) 5 family members	448:487	Homology alignment analysis indicated that ManAC belonged to glycosyl hydrolase (GH) 5 family members.					
34489044	6	37	theme	ManAC	911:915	arg1	activity					899:906	the activity	895:906	the activity of ManAC	895:915	The recombinant ManAC exhibited maximal activity at pH 2.5 and 70 °C, and it was acid tolerant at a pH range of 2.0-6.0 and thermostable under 60 °C. Meanwhile, the activity of ManAC was not significantly affected by various metal ions, except for Mg2+ and Ag2+.					
34489044	1	38	theme	renewable	242:250	arg1	release					182:188	the release	178:188	the release	178:188	β-Mannanases hydrolyze lignocellulosic biomass with the release of mannan oligosaccharides, which are considered as renewable resource in higher plants.					
34489044	1	38	theme	renewable	242:250	arg1	resource					252:259	renewable resource	242:259	renewable resource in higher plants	242:276	β-Mannanases hydrolyze lignocellulosic biomass with the release of mannan oligosaccharides, which are considered as renewable resource in higher plants.					
34489044	8	39	theme	thermophilic	1183:1194	arg1	characteristics					1213:1227	These thermophilic and acidophilicc characteristics	1177:1227	These thermophilic and acidophilicc characteristics	1177:1227	These thermophilic and acidophilicc characteristics is better than most extreme β-mannanase.					
34489044	3	40	theme	family	474:479	arg1	members					481:487	glycosyl hydrolase (GH) 5 family members	448:487	glycosyl hydrolase (GH) 5 family members	448:487	Homology alignment analysis indicated that ManAC belonged to glycosyl hydrolase (GH) 5 family members.					
34489044	3	41	theme	hydrolase	457:465	arg1	members					481:487	glycosyl hydrolase (GH) 5 family members	448:487	glycosyl hydrolase (GH) 5 family members	448:487	Homology alignment analysis indicated that ManAC belonged to glycosyl hydrolase (GH) 5 family members.					
34489044	5	42	theme	catalytic	705:713	arg1	ManAC					727:731	ManAC	727:731	ManAC	727:731	Glu232 and Glu340, proton donor and nucleophile, formed the catalytic residues of ManAC.					
34489044	5	42	theme	catalytic	705:713	arg1	residues					715:722	the catalytic residues	701:722	the catalytic residues of ManAC	701:731	Glu232 and Glu340, proton donor and nucleophile, formed the catalytic residues of ManAC.					
34489044	9	43	theme	excellent	1345:1353	arg1	properties					1355:1364	these excellent properties	1339:1364	these excellent properties of ManAC	1339:1373	As the first reported mannanse from Aspergillus calidoustus (ManAC), these excellent properties of ManAC strongly promote the synthesis of mannooligosaccharides which have potential for food and feed industrial applications.					
34489044	4	44	theme	ManAC	638:642	arg1	ManAC					638:642	ManAC	638:642	ManAC	638:642	The analysis of structural homologous model revealed that five residues, Arg116, Asn231, His305, Tyr307, and Trp370, constituted the active site of ManAC.					
34489044	4	44	theme	ManAC	638:642	arg1	site					630:633	the active site	619:633	the active site of ManAC	619:642	The analysis of structural homologous model revealed that five residues, Arg116, Asn231, His305, Tyr307, and Trp370, constituted the active site of ManAC.					
34489044	0	45	theme	thermophilic	55:66	arg1	β-mannanase					84:94	a novel thermophilic and acidophilic β-mannanase	47:94	a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus	47:123	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.					
34489044	5	46	theme	proton	664:669	arg1	donor					671:675	proton donor	664:675	proton donor	664:675	Glu232 and Glu340, proton donor and nucleophile, formed the catalytic residues of ManAC.					
34489044	5	46	theme	proton	664:669	arg1	Glu232					645:650	Glu232	645:650	Glu232	645:650	Glu232 and Glu340, proton donor and nucleophile, formed the catalytic residues of ManAC.					
34489044	1	47	from	resource	252:259	arg1	plants					271:276	higher plants	264:276	higher plants	264:276	β-Mannanases hydrolyze lignocellulosic biomass with the release of mannan oligosaccharides, which are considered as renewable resource in higher plants.					
34489044	7	48	theme	recombinant	1001:1011	arg1	ManAC					1013:1017	The recombinant ManAC	997:1017	The recombinant ManAC	997:1017	The recombinant ManAC exhibited the highest β-mannanase activity towards locust bean gum (669.7 U/mg) with the Km and Vmax values of 3.4 mg/mL and 982.4 μmol/min/mg, respectively.					
34489044	0	49	theme	novel	49:53	arg1	β-mannanase					84:94	a novel thermophilic and acidophilic β-mannanase	47:94	a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus	47:123	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.					
34489044	6	50	theme	recombinant	738:748	arg1	ManAC					750:754	The recombinant ManAC	734:754	The recombinant ManAC	734:754	The recombinant ManAC exhibited maximal activity at pH 2.5 and 70 °C, and it was acid tolerant at a pH range of 2.0-6.0 and thermostable under 60 °C. Meanwhile, the activity of ManAC was not significantly affected by various metal ions, except for Mg2+ and Ag2+.					
34489044	1	51	theme	lignocellulosic	149:163	arg1	biomass					165:171	lignocellulosic biomass	149:171	lignocellulosic biomass	149:171	β-Mannanases hydrolyze lignocellulosic biomass with the release of mannan oligosaccharides, which are considered as renewable resource in higher plants.					
34489044	0	52	from	calidoustus	113:123	arg1	β-mannanase					84:94	a novel thermophilic and acidophilic β-mannanase	47:94	a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus	47:123	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.					
34489044	0	52	from	calidoustus	113:123	arg1	characterization					27:42	Biochemical and structural characterization	0:42	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.	0:124	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.					
34489044	8	53	theme	extreme	1249:1255	arg1	β-mannanase					1257:1267	most extreme β-mannanase	1244:1267	most extreme β-mannanase	1244:1267	These thermophilic and acidophilicc characteristics is better than most extreme β-mannanase.					
34489044	1	54	theme	higher	264:269	arg1	plants					271:276	higher plants	264:276	higher plants	264:276	β-Mannanases hydrolyze lignocellulosic biomass with the release of mannan oligosaccharides, which are considered as renewable resource in higher plants.					
34489044	0	55	theme	acidophilic	72:82	arg1	β-mannanase					84:94	a novel thermophilic and acidophilic β-mannanase	47:94	a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus	47:123	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.					
34489044	4	56	theme	active	623:628	arg1	ManAC					638:642	ManAC	638:642	ManAC	638:642	The analysis of structural homologous model revealed that five residues, Arg116, Asn231, His305, Tyr307, and Trp370, constituted the active site of ManAC.					
34489044	4	56	theme	active	623:628	arg1	site					630:633	the active site	619:633	the active site of ManAC	619:642	The analysis of structural homologous model revealed that five residues, Arg116, Asn231, His305, Tyr307, and Trp370, constituted the active site of ManAC.					
34489044	0	57	from	characterization	27:42	arg1	calidoustus					113:123	Aspergillus calidoustus	101:123	Aspergillus calidoustus	101:123	Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus.					
34489044	6	58	from	range	837:841	arg1	tolerant					820:827	tolerant	820:827	tolerant	820:827	The recombinant ManAC exhibited maximal activity at pH 2.5 and 70 °C, and it was acid tolerant at a pH range of 2.0-6.0 and thermostable under 60 °C. Meanwhile, the activity of ManAC was not significantly affected by various metal ions, except for Mg2+ and Ag2+.					
34174476	17	0	theme	reported	2723:2730	arg1	glycosylation					2769:2781	the unusual glycosylation	2757:2781	the unusual glycosylation	2757:2781	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	17	0	theme	reported	2723:2730	arg1	modifications					2732:2744	The reported modifications	2719:2744	The reported modifications	2719:2744	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	14	1	theme	behavioural	2400:2410	arg1	responses					2412:2420	physiological and behavioural responses	2382:2420	physiological and behavioural responses	2382:2420	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	1	2	theme	enzyme	260:265	arg1	Peptides					157:164	Peptides	157:164	Peptides present in the seminal fluid of Drosophila melanogaster	157:220	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	1	2	theme	enzyme	260:265	arg1	inhibitors					267:276	enzyme inhibitors	260:276	enzyme inhibitors	260:276	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	9	3	link	O-linked	1453:1460	arg1	hexosamine					1471:1480	an O-linked N-acetyl hexosamine	1450:1480	an O-linked N-acetyl hexosamine	1450:1480	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	9	3	link	O-linked	1453:1460	arg1	hexose					1485:1490	a hexose	1483:1490	a hexose	1483:1490	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	9	3	link	O-linked	1453:1460	arg1	phosphoethanolamine					1508:1526	phosphoethanolamine	1508:1526	phosphoethanolamine	1508:1526	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	7	4	theme	extensive	1124:1132	arg1	modifications					1153:1165	more extensive post-translational modifications	1119:1165	more extensive post-translational modifications	1119:1165	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	13	5	theme	post-translational	1967:1984	arg1	important					2047:2055	important	2047:2055	important	2047:2055	We speculate that the post-translational modifications that we report for these gene products will be important not only for a biological function, but also for metabolic stability and might also facilitate transport across tissue barriers, such as the blood-brain barrier of the female insect.					
34174476	13	5	theme	post-translational	1967:1984	arg1	modifications					1986:1998	the post-translational modifications	1963:1998	the post-translational modifications that we report for these gene products	1963:2037	We speculate that the post-translational modifications that we report for these gene products will be important not only for a biological function, but also for metabolic stability and might also facilitate transport across tissue barriers, such as the blood-brain barrier of the female insect.					
34174476	9	6	theme	O-linked	1453:1460	arg1	hexosamine					1471:1480	an O-linked N-acetyl hexosamine	1450:1480	an O-linked N-acetyl hexosamine	1450:1480	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	9	6	theme	O-linked	1453:1460	arg1	hexose					1485:1490	a hexose	1483:1490	a hexose	1483:1490	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	9	6	theme	O-linked	1453:1460	arg1	phosphoethanolamine					1508:1526	phosphoethanolamine	1508:1526	phosphoethanolamine	1508:1526	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	4	7	theme	peptides	698:705	arg1	structures					665:674	the native structures	654:674	the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster	654:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	1	8	attach	present	166:172	arg2	Peptides					157:164	Peptides	157:164	Peptides present in the seminal fluid of Drosophila melanogaster	157:220	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	1	8	attach	present	166:172	arg2	agents					252:257	antimicrobial agents	238:257	antimicrobial agents	238:257	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	1	8	attach	present	166:172	arg2	inhibitors					267:276	enzyme inhibitors	260:276	enzyme inhibitors	260:276	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	1	8	attach	present	166:172	arg2	pheromones					285:294	as pheromones	282:294	as pheromones	282:294	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	1	8	attach	present	166:172	arg1	fluid					189:193	the seminal fluid	177:193	the seminal fluid of Drosophila melanogaster	177:220	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	0	9	with	glycopeptide	100:111	arg1	glycosylation					142:154	an unusual zwitterionic glycosylation	118:154	an unusual zwitterionic glycosylation	118:154	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	0	10	theme	Mass	0:3	arg1	characterisation					19:34	Mass spectrometric characterisation	0:34	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.	0:155	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	14	11	dep	SIGNIFICANCE	2251:2262	arg1	function					2307:2314	function	2307:2314	function	2307:2314	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	10	12	theme	recent	1615:1620	arg1	andropin					1569:1576	andropin	1569:1576	andropin	1569:1576	A survey of various genomes showed that andropin, CG42782, and Acp54A1 are relatively recent genes and are restricted to the melanogaster subgroup.					
34174476	10	12	theme	recent	1615:1620	arg1	genes					1622:1626	relatively recent genes	1604:1626	relatively recent genes	1604:1626	A survey of various genomes showed that andropin, CG42782, and Acp54A1 are relatively recent genes and are restricted to the melanogaster subgroup.					
34174476	10	12	theme	recent	1615:1620	arg1	CG42782					1579:1585	CG42782	1579:1585	CG42782	1579:1585	A survey of various genomes showed that andropin, CG42782, and Acp54A1 are relatively recent genes and are restricted to the melanogaster subgroup.					
34174476	10	12	theme	recent	1615:1620	arg1	Acp54A1					1592:1598	Acp54A1	1592:1598	Acp54A1	1592:1598	A survey of various genomes showed that andropin, CG42782, and Acp54A1 are relatively recent genes and are restricted to the melanogaster subgroup.					
34174476	14	13	theme	as	2357:2358	arg1	pheromones					2360:2369	as pheromones	2357:2369	as pheromones	2357:2369	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	13	theme	as	2357:2358	arg1	peptides					2279:2286	Seminal fluid peptides	2265:2286	Seminal fluid peptides of D. melanogaster	2265:2305	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	3	14	theme	standard	615:622	arg1	data					634:637	standard proteomic data	615:637	standard proteomic data	615:637	However, this information is often lacking and cannot be gleaned from just gene sequences and standard proteomic data.					
34174476	13	15	theme	metabolic	2106:2114	arg1	stability					2116:2124	metabolic stability	2106:2124	metabolic stability	2106:2124	We speculate that the post-translational modifications that we report for these gene products will be important not only for a biological function, but also for metabolic stability and might also facilitate transport across tissue barriers, such as the blood-brain barrier of the female insect.					
34174476	6	16	from	Met75C	1039:1044	arg1	presence					994:1001	the presence	990:1001	the presence of a penultimate Pro in CG42782 and Met75C	990:1044	In addition, the presence of a penultimate Pro in CG42782 and Met75C will help prevent degradation by carboxypeptidases.					
34174476	4	17	theme	seminal	684:690	arg1	andropin					708:715	andropin	708:715	andropin	708:715	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	17	theme	seminal	684:690	arg1	peptides					698:705	four seminal fluid peptides	679:705	four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster	679:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	17	theme	seminal	684:690	arg1	Met75C					727:732	Met75C	727:732	Met75C	727:732	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	17	theme	seminal	684:690	arg1	CG42782					718:724	CG42782	718:724	CG42782	718:724	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	17	theme	seminal	684:690	arg1	Acp54A1					738:744	Acp54A1	738:744	Acp54A1	738:744	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	0	18	theme	unusual	121:127	arg1	glycosylation					142:154	an unusual zwitterionic glycosylation	118:154	an unusual zwitterionic glycosylation	118:154	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	5	19	theme	cyclic	858:863	arg1	structure					865:873	a cyclic structure	856:873	a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability	856:974	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	7	20	attach	attachment	1232:1241	arg1	chain					1280:1284	the side chain	1271:1284	the side chain of Thr4	1271:1292	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	7	20	attach	attachment	1232:1241	arg2	residue					1216:1222	an N-terminal pyroglutamyl residue	1189:1222	an N-terminal pyroglutamyl residue	1189:1222	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	7	20	attach	attachment	1232:1241	arg2	O-glycan					1259:1266	a mucin-like O-glycan	1246:1266	a mucin-like O-glycan	1246:1266	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	14	21	theme	enzyme	2335:2340	arg1	peptides					2279:2286	Seminal fluid peptides	2265:2286	Seminal fluid peptides of D. melanogaster	2265:2305	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	21	theme	enzyme	2335:2340	arg1	inhibitors					2342:2351	enzyme inhibitors	2335:2351	enzyme inhibitors	2335:2351	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	17	22	theme	class	2829:2833	arg1	knowledge					2801:2809	our knowledge	2797:2809	our knowledge of this important class of peptides in this model organism, par excellence	2797:2884	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	11	23	theme	species	1735:1741	arg1	groups					1743:1748	the obscura species groups	1723:1748	the obscura species groups	1723:1748	Met75C, however, was also found in members of the obscura species groups and in Scaptodrosophila lebanonensis.					
34174476	6	24	theme	penultimate	1008:1018	arg1	Pro					1020:1022	a penultimate Pro	1006:1022	a penultimate Pro in CG42782 and Met75C	1006:1044	In addition, the presence of a penultimate Pro in CG42782 and Met75C will help prevent degradation by carboxypeptidases.					
34174476	17	25	theme	peptides	2838:2845	arg1	class					2829:2833	this important class	2814:2833	this important class of peptides in this model organism	2814:2868	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	17	25	theme	peptides	2838:2845	arg1	excellence					2875:2884	par excellence	2871:2884	par excellence	2871:2884	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	6	26	from	Pro	1020:1022	arg1	Met75C					1039:1044	Met75C	1039:1044	Met75C	1039:1044	In addition, the presence of a penultimate Pro in CG42782 and Met75C will help prevent degradation by carboxypeptidases.					
34174476	6	26	from	Pro	1020:1022	arg1	CG42782					1027:1033	CG42782	1027:1033	CG42782	1027:1033	In addition, the presence of a penultimate Pro in CG42782 and Met75C will help prevent degradation by carboxypeptidases.					
34174476	0	27	theme	ejaculatory	70:80	arg1	duct					82:85	the male ejaculatory duct	61:85	the male ejaculatory duct	61:85	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	1	28	theme	post-mated	355:364	arg1	female					366:371	the post-mated female	351:371	the post-mated female	351:371	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	15	29	theme	structure	2561:2569	arg1	knowledge					2525:2533	knowledge	2525:2533	knowledge not only of their primary structure, but also of their post-translational modification	2525:2620	A fuller understanding of how these peptides influence reproduction requires knowledge not only of their primary structure, but also of their post-translational modification.					
34174476	1	30	theme	present	166:172	arg1	inhibitors					267:276	enzyme inhibitors	260:276	enzyme inhibitors	260:276	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	1	30	theme	present	166:172	arg1	agents					252:257	antimicrobial agents	238:257	antimicrobial agents	238:257	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	1	30	theme	present	166:172	arg1	Peptides					157:164	Peptides	157:164	Peptides present in the seminal fluid of Drosophila melanogaster	157:220	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	1	30	theme	present	166:172	arg1	pheromones					285:294	as pheromones	282:294	as pheromones	282:294	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	6	31	from	CG42782	1027:1033	arg1	presence					994:1001	the presence	990:1001	the presence of a penultimate Pro in CG42782 and Met75C	990:1044	In addition, the presence of a penultimate Pro in CG42782 and Met75C will help prevent degradation by carboxypeptidases.					
34174476	14	32	theme	physiological	2382:2394	arg1	responses					2412:2420	physiological and behavioural responses	2382:2420	physiological and behavioural responses	2382:2420	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	4	33	theme	D.	781:782	arg1	duct					768:771	the ejaculatory duct	752:771	the ejaculatory duct of male D. melanogaster	752:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	14	34	theme	D.	2291:2292	arg1	pheromones					2360:2369	as pheromones	2357:2369	as pheromones	2357:2369	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	34	theme	D.	2291:2292	arg1	peptides					2279:2286	Seminal fluid peptides	2265:2286	Seminal fluid peptides of D. melanogaster	2265:2305	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	34	theme	D.	2291:2292	arg1	antimicrobials					2319:2332	antimicrobials	2319:2332	antimicrobials	2319:2332	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	34	theme	D.	2291:2292	arg1	inhibitors					2342:2351	enzyme inhibitors	2335:2351	enzyme inhibitors	2335:2351	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	11	35	theme	Scaptodrosophila	1757:1772	arg1	lebanonensis					1774:1785	Scaptodrosophila lebanonensis	1757:1785	Scaptodrosophila lebanonensis	1757:1785	Met75C, however, was also found in members of the obscura species groups and in Scaptodrosophila lebanonensis.					
34174476	6	36	from	presence	994:1001	arg1	Met75C					1039:1044	Met75C	1039:1044	Met75C	1039:1044	In addition, the presence of a penultimate Pro in CG42782 and Met75C will help prevent degradation by carboxypeptidases.					
34174476	6	36	from	presence	994:1001	arg1	CG42782					1027:1033	CG42782	1027:1033	CG42782	1027:1033	In addition, the presence of a penultimate Pro in CG42782 and Met75C will help prevent degradation by carboxypeptidases.					
34174476	8	37	theme	secreted	1376:1383	arg1	peptide					1385:1391	the secreted peptide	1372:1391	the secreted peptide	1372:1391	Both of these modifications are expected to further enhance the stability of the secreted peptide.					
34174476	0	38	theme	male	65:68	arg1	duct					82:85	the male ejaculatory duct	61:85	the male ejaculatory duct	61:85	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	4	39	theme	ejaculatory	756:766	arg1	duct					768:771	the ejaculatory duct	752:771	the ejaculatory duct of male D. melanogaster	752:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	14	40	theme	Seminal	2265:2271	arg1	pheromones					2360:2369	as pheromones	2357:2369	as pheromones	2357:2369	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	40	theme	Seminal	2265:2271	arg1	peptides					2279:2286	Seminal fluid peptides	2265:2286	Seminal fluid peptides of D. melanogaster	2265:2305	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	40	theme	Seminal	2265:2271	arg1	antimicrobials					2319:2332	antimicrobials	2319:2332	antimicrobials	2319:2332	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	40	theme	Seminal	2265:2271	arg1	inhibitors					2342:2351	enzyme inhibitors	2335:2351	enzyme inhibitors	2335:2351	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	10	41	theme	melanogaster	1654:1665	arg1	subgroup					1667:1674	the melanogaster subgroup	1650:1674	the melanogaster subgroup	1650:1674	A survey of various genomes showed that andropin, CG42782, and Acp54A1 are relatively recent genes and are restricted to the melanogaster subgroup.					
34174476	7	42	theme	residue	1216:1222	arg1	formation					1176:1184	the formation	1172:1184	the formation of an N-terminal pyroglutamyl residue	1172:1222	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	7	42	theme	residue	1216:1222	arg1	attachment					1232:1241	the attachment	1228:1241	the attachment of a mucin-like O-glycan to the side chain of Thr4	1228:1292	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	1	43	theme	as	282:283	arg1	Peptides					157:164	Peptides	157:164	Peptides present in the seminal fluid of Drosophila melanogaster	157:220	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	1	43	theme	as	282:283	arg1	pheromones					285:294	as pheromones	282:294	as pheromones	282:294	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	11	44	located	found	1703:1707	arg2	Met75C					1677:1682	Met75C	1677:1682	Met75C	1677:1682	Met75C, however, was also found in members of the obscura species groups and in Scaptodrosophila lebanonensis.					
34174476	11	44	located	found	1703:1707	arg1	members					1712:1718	members	1712:1718	members of the obscura species groups	1712:1748	Met75C, however, was also found in members of the obscura species groups and in Scaptodrosophila lebanonensis.					
34174476	7	45	theme	N-terminal	1192:1201	arg1	residue					1216:1222	an N-terminal pyroglutamyl residue	1189:1222	an N-terminal pyroglutamyl residue	1189:1222	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	13	46	theme	female	2225:2230	arg1	insect					2232:2237	the female insect	2221:2237	the female insect	2221:2237	We speculate that the post-translational modifications that we report for these gene products will be important not only for a biological function, but also for metabolic stability and might also facilitate transport across tissue barriers, such as the blood-brain barrier of the female insect.					
34174476	12	47	theme	gene	1865:1868	arg1	duplication					1870:1880	tandem gene duplication	1858:1880	tandem gene duplication	1858:1880	Andropin is related to the cecropin gene family and probably arose by tandem gene duplication, whereas CG42782, Met75C and Acp54A1 possibly emerged de novo.					
34174476	17	48	from	organism	2861:2868	arg1	class					2829:2833	this important class	2814:2833	this important class of peptides in this model organism	2814:2868	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	17	48	from	organism	2861:2868	arg1	excellence					2875:2884	par excellence	2871:2884	par excellence	2871:2884	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	0	49	gly	glycopeptide	100:111	arg2	glycopeptide					100:111	a glycopeptide	98:111	a glycopeptide with an unusual zwitterionic glycosylation	98:154	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	0	50	gly	glycosylation	142:154	arg1	glycopeptide					100:111	a glycopeptide	98:111	a glycopeptide with an unusual zwitterionic glycosylation	98:154	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	5	51	theme	mature	802:807	arg1	peptides					837:844	The mature CG42782, Met75C and Acp54A1 peptides	798:844	The mature CG42782, Met75C and Acp54A1 peptides each	798:849	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	1	52	theme	physiological	308:320	arg1	responses					338:346	physiological and behavioural responses	308:346	physiological and behavioural responses	308:346	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	12	53	theme	gene	1824:1827	arg1	family					1829:1834	the cecropin gene family	1811:1834	the cecropin gene family	1811:1834	Andropin is related to the cecropin gene family and probably arose by tandem gene duplication, whereas CG42782, Met75C and Acp54A1 possibly emerged de novo.					
34174476	2	54	theme	structures	509:518	arg1	knowledge					480:488	detailed knowledge	471:488	detailed knowledge of their molecular structures	471:518	Understanding the molecular interactions by which these peptides influence reproduction requires detailed knowledge of their molecular structures.					
34174476	10	55	theme	genomes	1549:1555	arg1	survey					1531:1536	A survey	1529:1536	A survey of various genomes	1529:1555	A survey of various genomes showed that andropin, CG42782, and Acp54A1 are relatively recent genes and are restricted to the melanogaster subgroup.					
34174476	13	56	theme	gene	2025:2028	arg1	products					2030:2037	these gene products	2019:2037	these gene products	2019:2037	We speculate that the post-translational modifications that we report for these gene products will be important not only for a biological function, but also for metabolic stability and might also facilitate transport across tissue barriers, such as the blood-brain barrier of the female insect.					
34174476	17	57	theme	par	2871:2873	arg1	class					2829:2833	this important class	2814:2833	this important class of peptides in this model organism	2814:2868	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	17	57	theme	par	2871:2873	arg1	excellence					2875:2884	par excellence	2871:2884	par excellence	2871:2884	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	0	58	theme	peptides	49:56	arg1	characterisation					19:34	Mass spectrometric characterisation	0:34	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.	0:155	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	1	59	theme	behavioural	326:336	arg1	responses					338:346	physiological and behavioural responses	308:346	physiological and behavioural responses	308:346	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	17	60	from	peptides	2838:2845	arg1	organism					2861:2868	this model organism	2850:2868	this model organism	2850:2868	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	11	61	theme	obscura	1727:1733	arg1	groups					1743:1748	the obscura species groups	1723:1748	the obscura species groups	1723:1748	Met75C, however, was also found in members of the obscura species groups and in Scaptodrosophila lebanonensis.					
34174476	13	62	theme	tissue	2169:2174	arg1	barriers					2176:2183	tissue barriers	2169:2183	tissue barriers	2169:2183	We speculate that the post-translational modifications that we report for these gene products will be important not only for a biological function, but also for metabolic stability and might also facilitate transport across tissue barriers, such as the blood-brain barrier of the female insect.					
34174476	13	62	theme	tissue	2169:2174	arg1	barrier					2210:2216	the blood-brain barrier	2194:2216	the blood-brain barrier of the female insect	2194:2237	We speculate that the post-translational modifications that we report for these gene products will be important not only for a biological function, but also for metabolic stability and might also facilitate transport across tissue barriers, such as the blood-brain barrier of the female insect.					
34174476	17	63	from	class	2829:2833	arg1	organism					2861:2868	this model organism	2850:2868	this model organism	2850:2868	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	2	64	theme	molecular	392:400	arg1	interactions					402:413	the molecular interactions	388:413	the molecular interactions by which these peptides influence reproduction	388:460	Understanding the molecular interactions by which these peptides influence reproduction requires detailed knowledge of their molecular structures.					
34174476	15	65	theme	modification	2609:2620	arg1	knowledge					2525:2533	knowledge	2525:2533	knowledge not only of their primary structure, but also of their post-translational modification	2525:2620	A fuller understanding of how these peptides influence reproduction requires knowledge not only of their primary structure, but also of their post-translational modification.					
34174476	9	66	theme	rare	1411:1414	arg1	structure					1429:1437	a rare zwitterionic structure	1409:1437	a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine	1409:1526	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	16	67	theme	proteomic	2703:2711	arg1	data					2713:2716	standard proteomic data	2694:2716	standard proteomic data	2694:2716	However, this information is often lacking and difficult to glean from standard proteomic data.					
34174476	7	68	theme	post-translational	1134:1151	arg1	modifications					1153:1165	more extensive post-translational modifications	1119:1165	more extensive post-translational modifications	1119:1165	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	5	69	dep	peptides	837:844	arg1	each					846:849	each	846:849	each	846:849	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	7	70	theme	side	1275:1278	arg1	chain					1280:1284	the side chain	1271:1284	the side chain of Thr4	1271:1292	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	9	71	contain	has	1405:1407	arg2	structure					1429:1437	a rare zwitterionic structure	1409:1437	a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine	1409:1526	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	9	71	contain	has	1405:1407	arg1	glycan					1398:1403	The glycan	1394:1403	The glycan	1394:1403	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	9	72	theme	N-acetyl	1462:1469	arg1	hexosamine					1471:1480	an O-linked N-acetyl hexosamine	1450:1480	an O-linked N-acetyl hexosamine	1450:1480	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	9	72	theme	N-acetyl	1462:1469	arg1	hexose					1485:1490	a hexose	1483:1490	a hexose	1483:1490	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	9	72	theme	N-acetyl	1462:1469	arg1	phosphoethanolamine					1508:1526	phosphoethanolamine	1508:1526	phosphoethanolamine	1508:1526	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
34174476	17	73	theme	important	2819:2827	arg1	class					2829:2833	this important class	2814:2833	this important class of peptides in this model organism	2814:2868	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	17	73	theme	important	2819:2827	arg1	excellence					2875:2884	par excellence	2871:2884	par excellence	2871:2884	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	13	74	theme	blood-brain	2198:2208	arg1	barrier					2210:2216	the blood-brain barrier	2194:2216	the blood-brain barrier of the female insect	2194:2237	We speculate that the post-translational modifications that we report for these gene products will be important not only for a biological function, but also for metabolic stability and might also facilitate transport across tissue barriers, such as the blood-brain barrier of the female insect.					
34174476	17	75	theme	unusual	2761:2767	arg1	glycosylation					2769:2781	the unusual glycosylation	2757:2781	the unusual glycosylation	2757:2781	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	4	76	theme	fluid	692:696	arg1	andropin					708:715	andropin	708:715	andropin	708:715	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	76	theme	fluid	692:696	arg1	peptides					698:705	four seminal fluid peptides	679:705	four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster	679:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	76	theme	fluid	692:696	arg1	Met75C					727:732	Met75C	727:732	Met75C	727:732	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	76	theme	fluid	692:696	arg1	CG42782					718:724	CG42782	718:724	CG42782	718:724	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	76	theme	fluid	692:696	arg1	Acp54A1					738:744	Acp54A1	738:744	Acp54A1	738:744	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	7	77	theme	mucin-like	1248:1257	arg1	O-glycan					1259:1266	a mucin-like O-glycan	1246:1266	a mucin-like O-glycan	1246:1266	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	1	78	theme	seminal	181:187	arg1	fluid					189:193	the seminal fluid	177:193	the seminal fluid of Drosophila melanogaster	177:220	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	13	79	theme	biological	2072:2081	arg1	function					2083:2090	a biological function	2070:2090	a biological function	2070:2090	We speculate that the post-translational modifications that we report for these gene products will be important not only for a biological function, but also for metabolic stability and might also facilitate transport across tissue barriers, such as the blood-brain barrier of the female insect.					
34174476	3	80	theme	gene	596:599	arg1	sequences					601:609	just gene sequences	591:609	just gene sequences	591:609	However, this information is often lacking and cannot be gleaned from just gene sequences and standard proteomic data.					
34174476	0	81	theme	zwitterionic	129:140	arg1	glycosylation					142:154	an unusual zwitterionic glycosylation	118:154	an unusual zwitterionic glycosylation	118:154	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	4	82	from	structures	665:674	arg1	duct					768:771	the ejaculatory duct	752:771	the ejaculatory duct of male D. melanogaster	752:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	1	83	theme	melanogaster	209:220	arg1	fluid					189:193	the seminal fluid	177:193	the seminal fluid of Drosophila melanogaster	177:220	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	3	84	theme	proteomic	624:632	arg1	data					634:637	standard proteomic data	615:637	standard proteomic data	615:637	However, this information is often lacking and cannot be gleaned from just gene sequences and standard proteomic data.					
34174476	5	85	theme	conformational	921:934	arg1	freedom					936:942	conformational freedom	921:942	conformational freedom	921:942	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	16	86	theme	standard	2694:2701	arg1	data					2713:2716	standard proteomic data	2694:2716	standard proteomic data	2694:2716	However, this information is often lacking and difficult to glean from standard proteomic data.					
34174476	7	87	theme	O-glycan	1259:1266	arg1	formation					1176:1184	the formation	1172:1184	the formation of an N-terminal pyroglutamyl residue	1172:1222	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	7	87	theme	O-glycan	1259:1266	arg1	attachment					1232:1241	the attachment	1228:1241	the attachment of a mucin-like O-glycan to the side chain of Thr4	1228:1292	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	1	88	from	fluid	189:193	arg1	present					166:172	present	166:172	present	166:172	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	5	89	theme	disulfide	887:895	arg1	bond					897:900	a disulfide bond	885:900	a disulfide bond	885:900	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	6	90	theme	Pro	1020:1022	arg1	presence					994:1001	the presence	990:1001	the presence of a penultimate Pro in CG42782 and Met75C	990:1044	In addition, the presence of a penultimate Pro in CG42782 and Met75C will help prevent degradation by carboxypeptidases.					
34174476	11	91	theme	groups	1743:1748	arg1	members					1712:1718	members	1712:1718	members of the obscura species groups	1712:1748	Met75C, however, was also found in members of the obscura species groups and in Scaptodrosophila lebanonensis.					
34174476	0	92	theme	duct	82:85	arg1	peptides					49:56	the major peptides	39:56	the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation	39:154	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	15	93	theme	fuller	2450:2455	arg1	understanding					2457:2469	A fuller understanding	2448:2469	A fuller understanding of how these peptides influence reproduction	2448:2514	A fuller understanding of how these peptides influence reproduction requires knowledge not only of their primary structure, but also of their post-translational modification.					
34174476	4	94	dep	D.	781:782	arg1	melanogaster					784:795	D. melanogaster	781:795	male D. melanogaster	776:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	1	95	from	present	166:172	arg1	fluid					189:193	the seminal fluid	177:193	the seminal fluid of Drosophila melanogaster	177:220	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	17	96	theme	model	2855:2859	arg1	organism					2861:2868	this model organism	2850:2868	this model organism	2850:2868	The reported modifications, including the unusual glycosylation, adds much to our knowledge of this important class of peptides in this model organism, par excellence.					
34174476	15	97	theme	primary	2553:2559	arg1	structure					2561:2569	their primary structure	2547:2569	their primary structure	2547:2569	A fuller understanding of how these peptides influence reproduction requires knowledge not only of their primary structure, but also of their post-translational modification.					
34174476	5	98	theme	Met75C	818:823	arg1	peptides					837:844	The mature CG42782, Met75C and Acp54A1 peptides	798:844	The mature CG42782, Met75C and Acp54A1 peptides each	798:849	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	14	99	theme	fluid	2273:2277	arg1	pheromones					2360:2369	as pheromones	2357:2369	as pheromones	2357:2369	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	99	theme	fluid	2273:2277	arg1	peptides					2279:2286	Seminal fluid peptides	2265:2286	Seminal fluid peptides of D. melanogaster	2265:2305	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	99	theme	fluid	2273:2277	arg1	antimicrobials					2319:2332	antimicrobials	2319:2332	antimicrobials	2319:2332	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	99	theme	fluid	2273:2277	arg1	inhibitors					2342:2351	enzyme inhibitors	2335:2351	enzyme inhibitors	2335:2351	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	0	100	theme	spectrometric	5:17	arg1	characterisation					19:34	Mass spectrometric characterisation	0:34	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.	0:155	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	4	101	from	duct	768:771	arg1	Acp54A1					738:744	Acp54A1	738:744	Acp54A1	738:744	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	101	from	duct	768:771	arg1	structures					665:674	the native structures	654:674	the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster	654:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	101	from	duct	768:771	arg1	CG42782					718:724	CG42782	718:724	CG42782	718:724	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	101	from	duct	768:771	arg1	Met75C					727:732	Met75C	727:732	Met75C	727:732	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	101	from	duct	768:771	arg1	peptides					698:705	four seminal fluid peptides	679:705	four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster	679:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	101	from	duct	768:771	arg1	andropin					708:715	andropin	708:715	andropin	708:715	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	5	102	contain	have	851:854	arg2	structure					865:873	a cyclic structure	856:873	a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability	856:974	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	5	102	contain	have	851:854	arg1	peptides					837:844	The mature CG42782, Met75C and Acp54A1 peptides	798:844	The mature CG42782, Met75C and Acp54A1 peptides each	798:849	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	8	103	theme	peptide	1385:1391	arg1	stability					1359:1367	the stability	1355:1367	the stability of the secreted peptide	1355:1391	Both of these modifications are expected to further enhance the stability of the secreted peptide.					
34174476	5	104	theme	Acp54A1	829:835	arg1	peptides					837:844	The mature CG42782, Met75C and Acp54A1 peptides	798:844	The mature CG42782, Met75C and Acp54A1 peptides each	798:849	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	5	105	theme	metabolic	956:964	arg1	stability					966:974	metabolic stability	956:974	metabolic stability	956:974	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	2	106	theme	detailed	471:478	arg1	knowledge					480:488	detailed knowledge	471:488	detailed knowledge of their molecular structures	471:518	Understanding the molecular interactions by which these peptides influence reproduction requires detailed knowledge of their molecular structures.					
34174476	14	107	theme	BIOLOGICAL	2240:2249	arg1	SIGNIFICANCE					2251:2262	BIOLOGICAL SIGNIFICANCE	2240:2262	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.	2240:2446	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	0	108	theme	major	43:47	arg1	peptides					49:56	the major peptides	39:56	the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation	39:154	Mass spectrometric characterisation of the major peptides of the male ejaculatory duct, including a glycopeptide with an unusual zwitterionic glycosylation.					
34174476	13	109	theme	insect	2232:2237	arg1	barrier					2210:2216	the blood-brain barrier	2194:2216	the blood-brain barrier of the female insect	2194:2237	We speculate that the post-translational modifications that we report for these gene products will be important not only for a biological function, but also for metabolic stability and might also facilitate transport across tissue barriers, such as the blood-brain barrier of the female insect.					
34174476	1	110	theme	antimicrobial	238:250	arg1	agents					252:257	antimicrobial agents	238:257	antimicrobial agents	238:257	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	1	110	theme	antimicrobial	238:250	arg1	Peptides					157:164	Peptides	157:164	Peptides present in the seminal fluid of Drosophila melanogaster	157:220	Peptides present in the seminal fluid of Drosophila melanogaster can function as antimicrobial agents, enzyme inhibitors and as pheromones that elicit physiological and behavioural responses in the post-mated female.					
34174476	5	111	theme	CG42782	809:815	arg1	peptides					837:844	The mature CG42782, Met75C and Acp54A1 peptides	798:844	The mature CG42782, Met75C and Acp54A1 peptides each	798:849	The mature CG42782, Met75C and Acp54A1 peptides each have a cyclic structure formed by a disulfide bond, which will reduce conformational freedom and enhance metabolic stability.					
34174476	12	112	theme	cecropin	1815:1822	arg1	family					1829:1834	the cecropin gene family	1811:1834	the cecropin gene family	1811:1834	Andropin is related to the cecropin gene family and probably arose by tandem gene duplication, whereas CG42782, Met75C and Acp54A1 possibly emerged de novo.					
34174476	2	113	theme	molecular	499:507	arg1	structures					509:518	their molecular structures	493:518	their molecular structures	493:518	Understanding the molecular interactions by which these peptides influence reproduction requires detailed knowledge of their molecular structures.					
34174476	10	114	theme	various	1541:1547	arg1	genomes					1549:1555	various genomes	1541:1555	various genomes	1541:1555	A survey of various genomes showed that andropin, CG42782, and Acp54A1 are relatively recent genes and are restricted to the melanogaster subgroup.					
34174476	12	115	theme	tandem	1858:1863	arg1	duplication					1870:1880	tandem gene duplication	1858:1880	tandem gene duplication	1858:1880	Andropin is related to the cecropin gene family and probably arose by tandem gene duplication, whereas CG42782, Met75C and Acp54A1 possibly emerged de novo.					
34174476	7	116	theme	Thr4	1289:1292	arg1	chain					1280:1284	the side chain	1271:1284	the side chain of Thr4	1271:1292	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	14	117	dep	D.	2291:2292	arg1	melanogaster					2294:2305	D. melanogaster	2291:2305	D. melanogaster	2291:2305	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	14	118	theme	post-mated	2429:2438	arg1	female					2440:2445	the post-mated female	2425:2445	the post-mated female	2425:2445	BIOLOGICAL SIGNIFICANCE: Seminal fluid peptides of D. melanogaster function as antimicrobials, enzyme inhibitors and as pheromones, eliciting physiological and behavioural responses in the post-mated female.					
34174476	15	119	theme	post-translational	2590:2607	arg1	modification					2609:2620	their post-translational modification	2584:2620	their post-translational modification	2584:2620	A fuller understanding of how these peptides influence reproduction requires knowledge not only of their primary structure, but also of their post-translational modification.					
34174476	4	120	dep	peptides	698:705	arg1	Acp54A1					738:744	Acp54A1	738:744	Acp54A1	738:744	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	120	dep	peptides	698:705	arg1	Met75C					727:732	Met75C	727:732	Met75C	727:732	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	120	dep	peptides	698:705	arg1	CG42782					718:724	CG42782	718:724	CG42782	718:724	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	120	dep	peptides	698:705	arg1	peptides					698:705	four seminal fluid peptides	679:705	four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster	679:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	120	dep	peptides	698:705	arg1	andropin					708:715	andropin	708:715	andropin	708:715	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	4	121	theme	native	658:663	arg1	structures					665:674	the native structures	654:674	the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster	654:795	We now report the native structures of four seminal fluid peptides (andropin, CG42782, Met75C and Acp54A1) from the ejaculatory duct of male D. melanogaster.					
34174476	7	122	theme	pyroglutamyl	1203:1214	arg1	residue					1216:1222	an N-terminal pyroglutamyl residue	1189:1222	an N-terminal pyroglutamyl residue	1189:1222	Met75C has undergone more extensive post-translational modifications with the formation of an N-terminal pyroglutamyl residue and the attachment of a mucin-like O-glycan to the side chain of Thr4.					
34174476	9	123	theme	zwitterionic	1416:1427	arg1	structure					1429:1437	a rare zwitterionic structure	1409:1437	a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine	1409:1526	The glycan has a rare zwitterionic structure comprising an O-linked N-acetyl hexosamine, a hexose and, unusually, phosphoethanolamine.					
32169901	0	0	theme	lactis	84:89	arg1	glycopolymers					101:113	Lactococcus lactis cell wall glycopolymers	72:113	Lactococcus lactis cell wall glycopolymers	72:113	Three distinct glycosylation pathways are involved in the decoration of Lactococcus lactis cell wall glycopolymers.					
32169901	4	1	from	addition	1113:1120	arg1	rhamnan					1145:1151	rhamnan	1145:1151	rhamnan	1145:1151	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	4	1	from	addition	1113:1120	arg1	pellicle					1172:1179	polysaccharide pellicle	1157:1179	polysaccharide pellicle	1157:1179	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	4	1	from	addition	1113:1120	arg1	components					1134:1143	the CWPS components rhamnan and polysaccharide pellicle (PSP)	1125:1185	the CWPS components rhamnan and polysaccharide pellicle (PSP)	1125:1185	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	5	2	theme	L.	1331:1332	arg1	genome					1341:1346	the L. lactis genome	1327:1346	the L. lactis genome	1327:1346	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	0	3	theme	Lactococcus	72:82	arg1	glycopolymers					101:113	Lactococcus lactis cell wall glycopolymers	72:113	Lactococcus lactis cell wall glycopolymers	72:113	Three distinct glycosylation pathways are involved in the decoration of Lactococcus lactis cell wall glycopolymers.					
32169901	3	4	from	systems	805:811	arg1	lactis					842:847	the bacterium Lactococcus lactis	816:847	the bacterium Lactococcus lactis	816:847	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	5	dep	similarities	874:885	arg1	basis					856:860	basis	856:860	basis	856:860	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	5	dep	similarities	874:885	arg1	the					852:854	the	852:854	the	852:854	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	5	6	theme	wall	1441:1444	arg1	glycopolymers					1446:1458	the three cell wall glycopolymers rhamnan, PSP, and LTA	1426:1480	the three cell wall glycopolymers rhamnan, PSP, and LTA	1426:1480	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	6	theme	wall	1441:1444	arg1	PSP					1469:1471	PSP	1469:1471	PSP	1469:1471	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	6	theme	wall	1441:1444	arg1	LTA					1478:1480	LTA	1478:1480	LTA	1478:1480	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	6	theme	wall	1441:1444	arg1	rhamnan					1460:1466	rhamnan	1460:1466	rhamnan	1460:1466	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	3	7	theme	sequence	865:872	arg1	similarities					874:885	sequence similarities	865:885	sequence similarities	865:885	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	0	8	theme	wall	96:99	arg1	glycopolymers					101:113	Lactococcus lactis cell wall glycopolymers	72:113	Lactococcus lactis cell wall glycopolymers	72:113	Three distinct glycosylation pathways are involved in the decoration of Lactococcus lactis cell wall glycopolymers.					
32169901	4	9	theme	CWPS	1129:1132	arg1	rhamnan					1145:1151	rhamnan	1145:1151	rhamnan	1145:1151	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	4	9	theme	CWPS	1129:1132	arg1	pellicle					1172:1179	polysaccharide pellicle	1157:1179	polysaccharide pellicle	1157:1179	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	4	9	theme	CWPS	1129:1132	arg1	components					1134:1143	the CWPS components rhamnan and polysaccharide pellicle (PSP)	1125:1185	the CWPS components rhamnan and polysaccharide pellicle (PSP)	1125:1185	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	5	10	theme	cell	1436:1439	arg1	glycopolymers					1446:1458	the three cell wall glycopolymers rhamnan, PSP, and LTA	1426:1480	the three cell wall glycopolymers rhamnan, PSP, and LTA	1426:1480	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	10	theme	cell	1436:1439	arg1	PSP					1469:1471	PSP	1469:1471	PSP	1469:1471	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	10	theme	cell	1436:1439	arg1	LTA					1478:1480	LTA	1478:1480	LTA	1478:1480	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	10	theme	cell	1436:1439	arg1	rhamnan					1460:1466	rhamnan	1460:1466	rhamnan	1460:1466	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	3	11	theme	potential	995:1003	arg1	candidates					1019:1028	potential TGS component candidates	995:1028	potential TGS component candidates	995:1028	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	11	theme	potential	995:1003	arg1	pairs					919:923	three gene pairs	908:923	three gene pairs	908:923	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	0	12	theme	cell	91:94	arg1	glycopolymers					101:113	Lactococcus lactis cell wall glycopolymers	72:113	Lactococcus lactis cell wall glycopolymers	72:113	Three distinct glycosylation pathways are involved in the decoration of Lactococcus lactis cell wall glycopolymers.					
32169901	6	13	theme	bacteriophage	1637:1649	arg1	predation					1651:1659	bacteriophage predation	1637:1659	bacteriophage predation	1637:1659	Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.					
32169901	4	14	theme	polysaccharide	1157:1170	arg1	pellicle					1172:1179	polysaccharide pellicle	1157:1179	polysaccharide pellicle	1157:1179	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	4	14	theme	polysaccharide	1157:1170	arg1	components					1134:1143	the CWPS components rhamnan and polysaccharide pellicle (PSP)	1125:1185	the CWPS components rhamnan and polysaccharide pellicle (PSP)	1125:1185	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	2	15	theme	decoration	304:313	arg1	process					315:321	such a decoration process	297:321	such a decoration process	297:321	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	5	16	dep	glycopolymers	1446:1458	arg1	glycopolymers					1446:1458	the three cell wall glycopolymers rhamnan, PSP, and LTA	1426:1480	the three cell wall glycopolymers rhamnan, PSP, and LTA	1426:1480	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	16	dep	glycopolymers	1446:1458	arg1	PSP					1469:1471	PSP	1469:1471	PSP	1469:1471	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	16	dep	glycopolymers	1446:1458	arg1	LTA					1478:1480	LTA	1478:1480	LTA	1478:1480	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	16	dep	glycopolymers	1446:1458	arg1	rhamnan					1460:1466	rhamnan	1460:1466	rhamnan	1460:1466	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	4	17	theme	side-chain	1102:1111	arg1	addition					1113:1120	Glc side-chain addition	1098:1120	Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively	1098:1199	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	1	18	theme	bacterial	184:192	arg1	wall					199:202	the bacterial cell wall	180:202	the bacterial cell wall	180:202	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity.					
32169901	0	19	theme	glycopolymers	101:113	arg1	decoration					58:67	the decoration	54:67	the decoration of Lactococcus lactis cell wall glycopolymers	54:113	Three distinct glycosylation pathways are involved in the decoration of Lactococcus lactis cell wall glycopolymers.					
32169901	6	20	dep	L.	1697:1698	arg1	lactis					1700:1705	lactis	1700:1705	lactis	1700:1705	Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.					
32169901	4	21	dep	components	1134:1143	arg1	rhamnan					1145:1151	rhamnan	1145:1151	rhamnan	1145:1151	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	4	21	dep	components	1134:1143	arg1	pellicle					1172:1179	polysaccharide pellicle	1157:1179	polysaccharide pellicle	1157:1179	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	4	21	dep	components	1134:1143	arg1	components					1134:1143	the CWPS components rhamnan and polysaccharide pellicle (PSP)	1125:1185	the CWPS components rhamnan and polysaccharide pellicle (PSP)	1125:1185	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	4	21	dep	components	1134:1143	arg1	PSP					1182:1184	PSP	1182:1184	PSP	1182:1184	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	1	22	theme	cell	194:197	arg1	wall					199:202	the bacterial cell wall	180:202	the bacterial cell wall	180:202	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity.					
32169901	0	23	theme	distinct	6:13	arg1	pathways					29:36	Three distinct glycosylation pathways	0:36	Three distinct glycosylation pathways	0:36	Three distinct glycosylation pathways are involved in the decoration of Lactococcus lactis cell wall glycopolymers.					
32169901	3	24	theme	methylation	759:769	arg1	analysis					771:778	methylation analysis	759:778	methylation analysis	759:778	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	2	25	theme	glycosylation	350:362	arg1	TGS					372:374	TGS	372:374	TGS	372:374	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	2	25	theme	glycosylation	350:362	arg1	system					364:369	a three-component glycosylation system	332:369	a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer	332:596	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	1	26	theme	wall	199:202	arg1	components					166:175	glycopolymer components	153:175	glycopolymer components of the bacterial cell wall	153:202	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity.					
32169901	3	27	theme	TGS	1005:1007	arg1	candidates					1019:1028	potential TGS component candidates	995:1028	potential TGS component candidates	995:1028	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	27	theme	TGS	1005:1007	arg1	pairs					919:923	three gene pairs	908:923	three gene pairs	908:923	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	2	28	theme	three-component	334:348	arg1	TGS					372:374	TGS	372:374	TGS	372:374	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	2	28	theme	three-component	334:348	arg1	system					364:369	a three-component glycosylation system	332:369	a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer	332:596	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	4	29	theme	lipoteichoic	1251:1262	arg1	acid					1264:1267	lipoteichoic acid	1251:1267	lipoteichoic acid (LTA)	1251:1273	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	4	29	theme	lipoteichoic	1251:1262	arg1	LTA					1270:1272	LTA	1270:1272	LTA	1270:1272	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	3	30	theme	component	1009:1017	arg1	candidates					1019:1028	potential TGS component candidates	995:1028	potential TGS component candidates	995:1028	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	30	theme	component	1009:1017	arg1	pairs					919:923	three gene pairs	908:923	three gene pairs	908:923	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	0	31	theme	glycosylation	15:27	arg1	pathways					29:36	Three distinct glycosylation pathways	0:36	Three distinct glycosylation pathways	0:36	Three distinct glycosylation pathways are involved in the decoration of Lactococcus lactis cell wall glycopolymers.					
32169901	5	32	dep	identified	1284:1293	arg1	cflA					1361:1364	cflA	1361:1364	cflA	1361:1364	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	32	dep	identified	1284:1293	arg1	llnz_02975					1349:1358	llnz_02975	1349:1358	llnz_02975	1349:1358	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	4	33	theme	acid	1264:1267	arg1	galactosylation					1232:1246	galactosylation	1232:1246	galactosylation of lipoteichoic acid (LTA)	1232:1273	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	4	34	theme	Glc	1098:1100	arg1	addition					1113:1120	Glc side-chain addition	1098:1120	Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively	1098:1199	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	2	35	theme	Und-P	464:468	arg1	glycosyltransferase					443:461	an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase	392:461	an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT)	392:472	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	2	35	theme	Und-P	464:468	arg1	GT					470:471	Und-P GT	464:471	Und-P GT	464:471	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	2	36	theme	polytopic	493:501	arg1	glycosyltransferase					503:521	a polytopic glycosyltransferase	491:521	a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer	491:596	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	2	36	theme	polytopic	493:501	arg1	GT					529:530	PolM GT	524:530	PolM GT	524:530	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	3	37	theme	cell	690:693	arg1	polysaccharides					711:725	cell wall-associated polysaccharides	690:725	cell wall-associated polysaccharides (CWPS)	690:732	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	37	theme	cell	690:693	arg1	CWPS					728:731	CWPS	728:731	CWPS	728:731	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	38	theme	bioinformatic	611:623	arg1	analyses					625:632	bioinformatic analyses	611:632	bioinformatic analyses	611:632	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	38	theme	bioinformatic	611:623	arg1	recombineering					651:664	CRISPR-assisted recombineering	635:664	CRISPR-assisted recombineering	635:664	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	38	theme	bioinformatic	611:623	arg1	analysis					678:685	structural analysis	667:685	structural analysis of cell wall-associated polysaccharides (CWPS)	667:732	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	1	39	theme	structural	225:234	arg1	diversity					236:244	their structural diversity	219:244	their structural diversity	219:244	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity.					
32169901	2	40	theme	sugar-activating	426:441	arg1	glycosyltransferase					443:461	an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase	392:461	an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT)	392:472	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	2	40	theme	sugar-activating	426:441	arg1	GT					470:471	Und-P GT	464:471	Und-P GT	464:471	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	2	41	theme	sugar	556:560	arg1	residues					562:569	sugar residues	556:569	sugar residues	556:569	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	3	42	theme	structural	667:676	arg1	analyses					625:632	bioinformatic analyses	611:632	bioinformatic analyses	611:632	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	42	theme	structural	667:676	arg1	analysis					678:685	structural analysis	667:685	structural analysis of cell wall-associated polysaccharides (CWPS)	667:732	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	4	43	theme	experimental	1035:1046	arg1	results					1048:1054	Our experimental results	1031:1054	Our experimental results	1031:1054	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	5	44	theme	lactis	1334:1339	arg1	genome					1341:1346	the L. lactis genome	1327:1346	the L. lactis genome	1327:1346	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	45	gly	glycosylation	1409:1421	arg1	glycopolymers					1446:1458	the three cell wall glycopolymers rhamnan, PSP, and LTA	1426:1480	the three cell wall glycopolymers rhamnan, PSP, and LTA	1426:1480	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	45	gly	glycosylation	1409:1421	arg1	PSP					1469:1471	PSP	1469:1471	PSP	1469:1471	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	45	gly	glycosylation	1409:1421	arg1	LTA					1478:1480	LTA	1478:1480	LTA	1478:1480	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	5	45	gly	glycosylation	1409:1421	arg1	rhamnan					1460:1466	rhamnan	1460:1466	rhamnan	1460:1466	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	6	46	theme	L.	1697:1698	arg1	resistance					1707:1716	L. lactis resistance	1697:1716	L. lactis resistance to bacteriocin	1697:1731	Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.					
32169901	5	47	theme	glycopolymers	1446:1458	arg1	glycosylation					1409:1421	the glycosylation	1405:1421	the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA	1405:1480	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	6	48	theme	rhamnan	1594:1600	arg1	glucosylation					1572:1584	glucosylation	1572:1584	glucosylation of both rhamnan and PSP	1572:1608	Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.					
32169901	3	49	theme	Und-P	968:972	arg1	GT					974:975	an Und-P GT	965:975	an Und-P GT	965:975	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	1	50	theme	Extracytoplasmic	116:131	arg1	decoration					139:148	Extracytoplasmic sugar decoration	116:148	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall	116:202	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity.					
32169901	3	51	theme	such	800:803	arg1	systems					805:811	three such systems	794:811	three such systems in the bacterium Lactococcus lactis	794:847	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	6	52	theme	LTA	1670:1672	arg1	galactosylation					1674:1688	LTA galactosylation	1670:1688	LTA galactosylation	1670:1688	Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.					
32169901	4	53	dep	involved	1086:1093	arg1	whereas					1202:1208	whereas	1202:1208	whereas	1202:1208	Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA).					
32169901	1	54	theme	sugar	133:137	arg1	decoration					139:148	Extracytoplasmic sugar decoration	116:148	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall	116:202	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity.					
32169901	3	55	theme	CRISPR-assisted	635:649	arg1	analyses					625:632	bioinformatic analyses	611:632	bioinformatic analyses	611:632	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	55	theme	CRISPR-assisted	635:649	arg1	recombineering					651:664	CRISPR-assisted recombineering	635:664	CRISPR-assisted recombineering	635:664	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	6	56	theme	PSP	1606:1608	arg1	glucosylation					1572:1584	glucosylation	1572:1584	glucosylation of both rhamnan and PSP	1572:1608	Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.					
32169901	2	57	theme	specific	576:583	arg1	glycopolymer					585:596	a specific glycopolymer	574:596	a specific glycopolymer	574:596	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	3	58	theme	wall-associated	695:709	arg1	polysaccharides					711:725	cell wall-associated polysaccharides	690:725	cell wall-associated polysaccharides (CWPS)	690:732	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	58	theme	wall-associated	695:709	arg1	CWPS					728:731	CWPS	728:731	CWPS	728:731	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	5	59	theme	potential	1297:1305	arg1	flippase					1307:1314	a potential flippase	1295:1314	a potential flippase encoded in the L. lactis genome	1295:1346	We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs.					
32169901	3	60	theme	polysaccharides	711:725	arg1	analyses					625:632	bioinformatic analyses	611:632	bioinformatic analyses	611:632	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	60	theme	polysaccharides	711:725	arg1	analysis					678:685	structural analysis	667:685	structural analysis of cell wall-associated polysaccharides (CWPS)	667:732	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	61	theme	PolM	983:986	arg1	GT					988:989	a PolM GT	981:989	a PolM GT	981:989	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	1	62	theme	glycopolymer	153:164	arg1	components					166:175	glycopolymer components	153:175	glycopolymer components of the bacterial cell wall	153:202	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity.					
32169901	3	63	theme	bacterium	820:828	arg1	lactis					842:847	the bacterium Lactococcus lactis	816:847	the bacterium Lactococcus lactis	816:847	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	2	64	theme	PolM	524:527	arg1	glycosyltransferase					503:521	a polytopic glycosyltransferase	491:521	a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer	491:596	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	2	64	theme	PolM	524:527	arg1	GT					529:530	PolM GT	524:530	PolM GT	524:530	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	3	65	theme	MALDI-TOF	742:750	arg1	MS					752:753	MALDI-TOF MS	742:753	MALDI-TOF MS	742:753	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	2	66	theme	molecular	262:270	arg1	mechanism					272:280	the molecular mechanism	258:280	the molecular mechanism that underpins such a decoration process	258:321	Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer.					
32169901	1	67	theme	components	166:175	arg1	decoration					139:148	Extracytoplasmic sugar decoration	116:148	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall	116:202	Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity.					
32169901	3	68	theme	gene	914:917	arg1	csdEF					944:948	csdEF	944:948	csdEF	944:948	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	68	theme	gene	914:917	arg1	csdAB					926:930	csdAB	926:930	csdAB	926:930	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	68	theme	gene	914:917	arg1	each					951:954	each	951:954	each	951:954	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	68	theme	gene	914:917	arg1	csdCD					933:937	csdCD	933:937	csdCD	933:937	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	68	theme	gene	914:917	arg1	candidates					1019:1028	potential TGS component candidates	995:1028	potential TGS component candidates	995:1028	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	68	theme	gene	914:917	arg1	pairs					919:923	three gene pairs	908:923	three gene pairs	908:923	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
32169901	3	69	theme	Lactococcus	830:840	arg1	lactis					842:847	the bacterium Lactococcus lactis	816:847	the bacterium Lactococcus lactis	816:847	Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall-associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates.					
33772592	0	0	theme	Pichia	64:69	arg1	pastoris					71:78	Pichia pastoris	64:78	Pichia pastoris	64:78	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	6	1	theme	thermal	850:856	arg1	stability					858:866	the thermal stability	846:866	the thermal stability of the CH2 domain	846:884	Although both forms of CH2 domain have essentially the same secondary structure, the presence of the glycan increased the thermal stability of the CH2 domain by about 5°C as determined from calorimetry.					
33772592	7	2	gly	glycosylated	944:955	arg1	domain					961:966	The purified glycosylated CH2 domain	931:966	The purified glycosylated CH2 domain	931:966	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	1	3	theme	recombinant	227:237	arg1	protein					239:245	recombinant protein	227:245	recombinant protein	227:245	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	7	4	theme	commercial	1084:1093	arg1	antibody					1100:1107	the commercial IgG1 antibody	1080:1107	the commercial IgG1 antibody	1080:1107	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	7	5	theme	polyclonal	977:986	arg1	antibodies					988:997	polyclonal antibodies	977:997	polyclonal antibodies	977:997	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	1	6	theme	popular	141:147	arg1	system					160:165	a popular eukaryotic system	139:165	a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin	139:293	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	1	6	theme	popular	141:147	arg1	pastoris					127:134	Pichia pastoris	120:134	Pichia pastoris	120:134	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	6	7	theme	CH2	751:753	arg1	domain					755:760	CH2 domain	751:760	CH2 domain	751:760	Although both forms of CH2 domain have essentially the same secondary structure, the presence of the glycan increased the thermal stability of the CH2 domain by about 5°C as determined from calorimetry.					
33772592	1	8	theme	protein	239:245	arg1	production					213:222	the fast, simple and inexpensive production	180:222	the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin	180:293	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	9	9	theme	CH2	1375:1377	arg1	domain					1379:1384	their CH2 domain	1369:1384	their CH2 domain	1369:1384	Therefore, these antibodies are candidates to develop a novel affinity material to purify human antibodies using their CH2 domain.					
33772592	4	10	dep	glycosylated	622:633	arg1	%					639:639	∼70%	636:639	∼70%	636:639	The procedure yields, simultaneously, a major glycosylated (∼70%) and non-glycosylated (∼30%) fractions.					
33772592	6	11	theme	CH2	875:877	arg1	domain					879:884	the CH2 domain	871:884	the CH2 domain	871:884	Although both forms of CH2 domain have essentially the same secondary structure, the presence of the glycan increased the thermal stability of the CH2 domain by about 5°C as determined from calorimetry.					
33772592	4	12	gly	non-glycosylated	646:661	arg1	fractions					670:678	non-glycosylated (∼30%) fractions	646:678	non-glycosylated (∼30%) fractions	646:678	The procedure yields, simultaneously, a major glycosylated (∼70%) and non-glycosylated (∼30%) fractions.					
33772592	6	13	theme	secondary	788:796	arg1	structure					798:806	essentially the same secondary structure	767:806	essentially the same secondary structure	767:806	Although both forms of CH2 domain have essentially the same secondary structure, the presence of the glycan increased the thermal stability of the CH2 domain by about 5°C as determined from calorimetry.					
33772592	8	14	theme	CH3	1192:1194	arg1	domain					1196:1201	CH3 domain	1192:1201	CH3 domain	1192:1201	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	8	14	theme	CH3	1192:1194	arg1	Fc					1212:1213	human Fc	1206:1213	human Fc	1206:1213	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	6	15	contain	have	762:765	arg1	forms					742:746	both forms	737:746	both forms of CH2 domain	737:760	Although both forms of CH2 domain have essentially the same secondary structure, the presence of the glycan increased the thermal stability of the CH2 domain by about 5°C as determined from calorimetry.					
33772592	6	15	contain	have	762:765	arg2	structure					798:806	essentially the same secondary structure	767:806	essentially the same secondary structure	767:806	Although both forms of CH2 domain have essentially the same secondary structure, the presence of the glycan increased the thermal stability of the CH2 domain by about 5°C as determined from calorimetry.					
33772592	7	16	theme	human	1067:1071	arg1	Fc					1073:1074	recombinant human Fc	1055:1074	recombinant human Fc	1055:1074	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	6	17	theme	same	783:786	arg1	structure					798:806	essentially the same secondary structure	767:806	essentially the same secondary structure	767:806	Although both forms of CH2 domain have essentially the same secondary structure, the presence of the glycan increased the thermal stability of the CH2 domain by about 5°C as determined from calorimetry.					
33772592	8	18	theme	Protein	1132:1138	arg1	G					1140:1140	Protein G	1132:1140	Protein G	1132:1140	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	3	19	theme	human	494:498	arg1	domain					504:509	human CH2 domain	494:509	human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l)	494:552	To accelerate the research of CH2 domain, we have established a procedure to highly express human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l) in yeast P. pastoris.					
33772592	3	19	theme	human	494:498	arg1	∼150 mg/l					512:520	∼150 mg/l	512:520	∼150 mg/l	512:520	To accelerate the research of CH2 domain, we have established a procedure to highly express human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l) in yeast P. pastoris.					
33772592	1	20	theme	eukaryotic	149:158	arg1	system					160:165	a popular eukaryotic system	139:165	a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin	139:293	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	1	20	theme	eukaryotic	149:158	arg1	pastoris					127:134	Pichia pastoris	120:134	Pichia pastoris	120:134	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	7	21	theme	CH2	1034:1036	arg1	domain					1038:1043	the CH2 domain	1030:1043	the CH2 domain	1030:1043	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	8	22	theme	kink	1157:1160	arg1	region					1162:1167	the kink region	1153:1167	the kink region between CH2 domain and CH3 domain of human Fc	1153:1213	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	3	23	theme	CH2	500:502	arg1	domain					504:509	human CH2 domain	494:509	human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l)	494:552	To accelerate the research of CH2 domain, we have established a procedure to highly express human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l) in yeast P. pastoris.					
33772592	3	23	theme	CH2	500:502	arg1	∼150 mg/l					512:520	∼150 mg/l	512:520	∼150 mg/l	512:520	To accelerate the research of CH2 domain, we have established a procedure to highly express human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l) in yeast P. pastoris.					
33772592	7	24	theme	IgG1	1095:1098	arg1	antibody					1100:1107	the commercial IgG1 antibody	1080:1107	the commercial IgG1 antibody	1080:1107	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	4	25	theme	non-glycosylated	646:661	arg1	fractions					670:678	non-glycosylated (∼30%) fractions	646:678	non-glycosylated (∼30%) fractions	646:678	The procedure yields, simultaneously, a major glycosylated (∼70%) and non-glycosylated (∼30%) fractions.					
33772592	7	26	dep	Fc	1073:1074	arg1	Rituxan					1109:1115	Rituxan	1109:1115	Rituxan	1109:1115	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	2	27	theme	promising	348:356	arg1	scaffold					358:365	a promising scaffold	346:365	a promising scaffold for developing novel therapeutics	346:399	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	2	27	theme	promising	348:356	arg1	G					335:335	human Immunoglobulin G	314:335	human Immunoglobulin G (IgG)	314:341	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	2	27	theme	promising	348:356	arg1	domain					304:309	The CH2 domain	296:309	The CH2 domain of human Immunoglobulin G (IgG)	296:341	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	0	28	theme	High-level	0:9	arg1	expression					11:20	High-level expression	0:20	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.	0:118	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	4	29	dep	non-glycosylated	646:661	arg1	%					667:667	∼30%	664:667	∼30%	664:667	The procedure yields, simultaneously, a major glycosylated (∼70%) and non-glycosylated (∼30%) fractions.					
33772592	2	30	theme	CH2	300:302	arg1	scaffold					358:365	a promising scaffold	346:365	a promising scaffold for developing novel therapeutics	346:399	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	2	30	theme	CH2	300:302	arg1	G					335:335	human Immunoglobulin G	314:335	human Immunoglobulin G (IgG)	314:341	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	2	30	theme	CH2	300:302	arg1	domain					304:309	The CH2 domain	296:309	The CH2 domain of human Immunoglobulin G (IgG)	296:341	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	8	31	theme	Fc	1212:1213	arg1	domain					1181:1186	CH2 domain	1177:1186	CH2 domain	1177:1186	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	8	31	theme	Fc	1212:1213	arg1	domain					1196:1201	CH3 domain	1192:1201	CH3 domain	1192:1201	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	8	31	theme	Fc	1212:1213	arg1	Fc					1212:1213	human Fc	1206:1213	human Fc	1206:1213	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	0	32	theme	human	25:29	arg1	domain					35:40	human CH2 domain	25:40	human CH2 domain	25:40	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	0	33	theme	antibodies	108:117	arg1	preparation					84:94	preparation	84:94	preparation of anti-CH2 antibodies	84:117	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	0	33	theme	antibodies	108:117	arg1	region					54:59	the Fc region	47:59	the Fc region in Pichia pastoris	47:78	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	8	34	theme	CH2	1177:1179	arg1	domain					1181:1186	CH2 domain	1177:1186	CH2 domain	1177:1186	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	8	34	theme	CH2	1177:1179	arg1	Fc					1212:1213	human Fc	1206:1213	human Fc	1206:1213	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	3	35	theme	domain	436:441	arg1	research					420:427	the research	416:427	the research of CH2 domain	416:441	To accelerate the research of CH2 domain, we have established a procedure to highly express human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l) in yeast P. pastoris.					
33772592	1	36	theme	human	281:285	arg1	albumin					287:293	human albumin	281:293	human albumin	281:293	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	0	37	theme	anti-CH2	99:106	arg1	antibodies					108:117	anti-CH2 antibodies	99:117	anti-CH2 antibodies	99:117	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	3	38	theme	P.	563:564	arg1	pastoris					566:573	yeast P. pastoris	557:573	yeast P. pastoris	557:573	To accelerate the research of CH2 domain, we have established a procedure to highly express human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l) in yeast P. pastoris.					
33772592	1	39	theme	fast	184:187	arg1	production					213:222	the fast, simple and inexpensive production	180:222	the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin	180:293	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	0	40	theme	domain	35:40	arg1	expression					11:20	High-level expression	0:20	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.	0:118	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	9	41	theme	human	1346:1350	arg1	antibodies					1352:1361	human antibodies	1346:1361	human antibodies	1346:1361	Therefore, these antibodies are candidates to develop a novel affinity material to purify human antibodies using their CH2 domain.					
33772592	7	42	theme	purified	935:942	arg1	domain					961:966	The purified glycosylated CH2 domain	931:966	The purified glycosylated CH2 domain	931:966	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	6	43	theme	domain	755:760	arg1	forms					742:746	both forms	737:746	both forms of CH2 domain	737:760	Although both forms of CH2 domain have essentially the same secondary structure, the presence of the glycan increased the thermal stability of the CH2 domain by about 5°C as determined from calorimetry.					
33772592	2	44	theme	novel	382:386	arg1	therapeutics					388:399	novel therapeutics	382:399	novel therapeutics	382:399	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	0	45	theme	CH2	31:33	arg1	domain					35:40	human CH2 domain	25:40	human CH2 domain	25:40	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	6	46	theme	domain	879:884	arg1	stability					858:866	the thermal stability	846:866	the thermal stability of the CH2 domain	846:884	Although both forms of CH2 domain have essentially the same secondary structure, the presence of the glycan increased the thermal stability of the CH2 domain by about 5°C as determined from calorimetry.					
33772592	0	47	from	region	54:59	arg1	expression					11:20	High-level expression	0:20	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.	0:118	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	0	47	from	region	54:59	arg1	pastoris					71:78	Pichia pastoris	64:78	Pichia pastoris	64:78	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	5	48	theme	high	715:718	arg1	purity					720:725	high purity	715:725	high purity	715:725	They can be easily separated with high purity.					
33772592	1	49	theme	simple	190:195	arg1	production					213:222	the fast, simple and inexpensive production	180:222	the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin	180:293	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	7	50	from	antibodies	988:997	arg1	mouse					1002:1006	mouse	1002:1006	mouse	1002:1006	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	6	51	theme	glycan	829:834	arg1	presence					813:820	the presence	809:820	the presence of the glycan	809:834	Although both forms of CH2 domain have essentially the same secondary structure, the presence of the glycan increased the thermal stability of the CH2 domain by about 5°C as determined from calorimetry.					
33772592	2	52	theme	G	335:335	arg1	scaffold					358:365	a promising scaffold	346:365	a promising scaffold for developing novel therapeutics	346:399	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	2	52	theme	G	335:335	arg1	G					335:335	human Immunoglobulin G	314:335	human Immunoglobulin G (IgG)	314:341	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	2	52	theme	G	335:335	arg1	domain					304:309	The CH2 domain	296:309	The CH2 domain of human Immunoglobulin G (IgG)	296:341	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	7	53	theme	glycosylated	944:955	arg1	domain					961:966	The purified glycosylated CH2 domain	931:966	The purified glycosylated CH2 domain	931:966	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	7	54	theme	CH2	957:959	arg1	domain					961:966	The purified glycosylated CH2 domain	931:966	The purified glycosylated CH2 domain	931:966	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
33772592	3	55	theme	human	534:538	arg1	Fc					540:541	human Fc	534:541	human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l)	494:552	To accelerate the research of CH2 domain, we have established a procedure to highly express human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l) in yeast P. pastoris.					
33772592	3	55	theme	human	534:538	arg1	∼30 mg/l					544:551	∼30 mg/l	544:551	∼30 mg/l	544:551	To accelerate the research of CH2 domain, we have established a procedure to highly express human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l) in yeast P. pastoris.					
33772592	9	56	theme	affinity	1318:1325	arg1	material					1327:1334	a novel affinity material	1310:1334	a novel affinity material to purify human antibodies	1310:1361	Therefore, these antibodies are candidates to develop a novel affinity material to purify human antibodies using their CH2 domain.					
33772592	2	57	theme	Immunoglobulin	320:333	arg1	IgG					338:340	IgG	338:340	IgG	338:340	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	2	57	theme	Immunoglobulin	320:333	arg1	G					335:335	human Immunoglobulin G	314:335	human Immunoglobulin G (IgG)	314:341	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	0	58	from	preparation	84:94	arg1	expression					11:20	High-level expression	0:20	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.	0:118	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	0	58	from	preparation	84:94	arg1	pastoris					71:78	Pichia pastoris	64:78	Pichia pastoris	64:78	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	8	59	theme	therapeutic	1234:1244	arg1	proteins					1246:1253	therapeutic proteins	1234:1253	therapeutic proteins	1234:1253	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	2	60	theme	human	314:318	arg1	IgG					338:340	IgG	338:340	IgG	338:340	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	2	60	theme	human	314:318	arg1	G					335:335	human Immunoglobulin G	314:335	human Immunoglobulin G (IgG)	314:341	The CH2 domain of human Immunoglobulin G (IgG) is a promising scaffold for developing novel therapeutics.					
33772592	8	61	theme	human	1206:1210	arg1	Fc					1212:1213	human Fc	1206:1213	human Fc	1206:1213	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	3	62	theme	yeast	557:561	arg1	pastoris					566:573	yeast P. pastoris	557:573	yeast P. pastoris	557:573	To accelerate the research of CH2 domain, we have established a procedure to highly express human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l) in yeast P. pastoris.					
33772592	8	63	used	used	1219:1222	arg2	G					1140:1140	Protein G	1132:1140	Protein G	1132:1140	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	8	63	used	used	1219:1222	arg2	A					1126:1126	Protein A	1118:1126	Protein A	1118:1126	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	1	64	theme	Pichia	120:125	arg1	system					160:165	a popular eukaryotic system	139:165	a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin	139:293	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	1	64	theme	Pichia	120:125	arg1	pastoris					127:134	Pichia pastoris	120:134	Pichia pastoris	120:134	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	1	65	theme	inexpensive	201:211	arg1	production					213:222	the fast, simple and inexpensive production	180:222	the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin	180:293	Pichia pastoris is a popular eukaryotic system employed for the fast, simple and inexpensive production of recombinant protein including biotherapeutics such as human albumin.					
33772592	3	66	theme	CH2	432:434	arg1	domain					436:441	CH2 domain	432:441	CH2 domain	432:441	To accelerate the research of CH2 domain, we have established a procedure to highly express human CH2 domain (∼150 mg/l) as well as human Fc (∼30 mg/l) in yeast P. pastoris.					
33772592	9	67	theme	novel	1312:1316	arg1	material					1327:1334	a novel affinity material	1310:1334	a novel affinity material to purify human antibodies	1310:1361	Therefore, these antibodies are candidates to develop a novel affinity material to purify human antibodies using their CH2 domain.					
33772592	0	68	theme	Fc	51:52	arg1	region					54:59	the Fc region	47:59	the Fc region in Pichia pastoris	47:78	High-level expression of human CH2 domain from the Fc region in Pichia pastoris and preparation of anti-CH2 antibodies.					
33772592	8	69	theme	Protein	1118:1124	arg1	A					1126:1126	Protein A	1118:1126	Protein A	1118:1126	Protein A and Protein G binding to the kink region between CH2 domain and CH3 domain of human Fc are used to purify therapeutic proteins.					
33772592	7	70	theme	recombinant	1055:1065	arg1	Fc					1073:1074	recombinant human Fc	1055:1074	recombinant human Fc	1055:1074	The purified glycosylated CH2 domain elicited polyclonal antibodies in mouse, recognizing not only the CH2 domain, but also recombinant human Fc and the commercial IgG1 antibody Rituxan.					
32535667	0	0	theme	glycoconjugate	80:93	arg1	vaccines					95:102	site-selective glycoconjugate vaccines	65:102	site-selective glycoconjugate vaccines using CRM197 as carrier protein	65:134	Click chemistry compared to thiol chemistry for the synthesis of site-selective glycoconjugate vaccines using CRM197 as carrier protein.					
32535667	1	1	theme	highly-defined	350:363	arg1	structures					388:397	highly-defined and well-characterized structures	350:397	highly-defined and well-characterized structures	350:397	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	3	2	theme	S.	553:554	arg1	protein					616:622	CRM197 carrier protein	601:622	CRM197 carrier protein	601:622	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	3	2	theme	S.	553:554	arg1	models					637:642	models	637:642	models	637:642	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	3	2	theme	S.	553:554	arg1	O-Antigen					587:595	S. Typhimurium lipopolysaccharide O-Antigen	553:595	S. Typhimurium lipopolysaccharide O-Antigen	553:595	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	0	3	theme	site-selective	65:78	arg1	vaccines					95:102	site-selective glycoconjugate vaccines	65:102	site-selective glycoconjugate vaccines using CRM197 as carrier protein	65:134	Click chemistry compared to thiol chemistry for the synthesis of site-selective glycoconjugate vaccines using CRM197 as carrier protein.					
32535667	2	4	from	site	498:501	arg1	protein					544:550	the carrier protein	532:550	the carrier protein	532:550	Herein, different conjugation methods were investigated with the aim of controlling glycosylation site and glycosylation density on the carrier protein.					
32535667	2	5	theme	carrier	536:542	arg1	protein					544:550	the carrier protein	532:550	the carrier protein	532:550	Herein, different conjugation methods were investigated with the aim of controlling glycosylation site and glycosylation density on the carrier protein.					
32535667	4	6	theme	reducing	744:751	arg1	unit					759:762	the terminal reducing sugar unit	731:762	the terminal reducing sugar unit of the O-Antigen chain	731:785	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	5	7	theme	linkers	976:982	arg1	number					966:971	a relative high number	950:971	a relative high number of linkers	950:982	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	1	8	theme	well-characterized	369:386	arg1	structures					388:397	highly-defined and well-characterized structures	350:397	highly-defined and well-characterized structures	350:397	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	3	9	theme	carrier	608:614	arg1	O-Antigen					587:595	S. Typhimurium lipopolysaccharide O-Antigen	553:595	S. Typhimurium lipopolysaccharide O-Antigen	553:595	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	3	9	theme	carrier	608:614	arg1	protein					616:622	CRM197 carrier protein	601:622	CRM197 carrier protein	601:622	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	3	9	theme	carrier	608:614	arg1	models					637:642	models	637:642	models	637:642	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	5	10	theme	click	991:995	arg1	chemistry					997:1005	click chemistry	991:1005	click chemistry	991:1005	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	1	11	theme	deeper	274:279	arg1	understanding					281:293	a deeper understanding	272:293	a deeper understanding of their mechanism of action	272:322	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	0	12	theme	vaccines	95:102	arg1	synthesis					52:60	the synthesis	48:60	the synthesis of site-selective glycoconjugate vaccines using CRM197 as carrier protein	48:134	Click chemistry compared to thiol chemistry for the synthesis of site-selective glycoconjugate vaccines using CRM197 as carrier protein.					
32535667	4	13	theme	sugar	753:757	arg1	unit					759:762	the terminal reducing sugar unit	731:762	the terminal reducing sugar unit of the O-Antigen chain	731:785	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	6	14	theme	immune	1310:1315	arg1	response					1317:1324	immune response	1310:1324	immune response	1310:1324	The study highlights click chemistry as a leading approach for the synthesis of well-defined glycoconjugates, useful to investigate the relationship between conjugate design and immune response.					
32535667	4	15	from	acids	806:810	arg1	protein					827:833	the carrier protein	815:833	the carrier protein	815:833	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	4	16	theme	carrier	819:825	arg1	protein					827:833	the carrier protein	815:833	the carrier protein	815:833	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	2	17	theme	conjugation	418:428	arg1	methods					430:436	different conjugation methods	408:436	different conjugation methods	408:436	Herein, different conjugation methods were investigated with the aim of controlling glycosylation site and glycosylation density on the carrier protein.					
32535667	2	18	theme	glycosylation	484:496	arg1	site					498:501	glycosylation site	484:501	glycosylation site	484:501	Herein, different conjugation methods were investigated with the aim of controlling glycosylation site and glycosylation density on the carrier protein.					
32535667	1	19	theme	main	173:176	arg1	parameters					178:187	the main parameters	169:187	the main parameters affecting immunogenicity of glycoconjugate vaccines	169:239	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	2	20	from	density	521:527	arg1	protein					544:550	the carrier protein	532:550	the carrier protein	532:550	Herein, different conjugation methods were investigated with the aim of controlling glycosylation site and glycosylation density on the carrier protein.					
32535667	3	21	dep	S.	553:554	arg1	Typhimurium					556:566	Typhimurium	556:566	Typhimurium	556:566	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	1	22	theme	parameters	178:187	arg1	parameters					178:187	the main parameters	169:187	the main parameters affecting immunogenicity of glycoconjugate vaccines	169:239	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	1	22	theme	parameters	178:187	arg1	one					162:164	one	162:164	one	162:164	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	0	23	theme	Click	0:4	arg1	chemistry					6:14	Click chemistry	0:14	Click chemistry	0:14	Click chemistry compared to thiol chemistry for the synthesis of site-selective glycoconjugate vaccines using CRM197 as carrier protein.					
32535667	6	24	theme	conjugate	1289:1297	arg1	design					1299:1304	conjugate design	1289:1304	conjugate design	1289:1304	The study highlights click chemistry as a leading approach for the synthesis of well-defined glycoconjugates, useful to investigate the relationship between conjugate design and immune response.					
32535667	4	25	theme	chain	781:785	arg1	unit					759:762	the terminal reducing sugar unit	731:762	the terminal reducing sugar unit of the O-Antigen chain	731:785	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	2	26	theme	different	408:416	arg1	methods					430:436	different conjugation methods	408:436	different conjugation methods	408:436	Herein, different conjugation methods were investigated with the aim of controlling glycosylation site and glycosylation density on the carrier protein.					
32535667	5	27	theme	carrier	896:902	arg1	protein					904:910	the carrier protein	892:910	the carrier protein	892:910	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	5	28	theme	O-Antigen	860:868	arg1	conjugation					870:880	O-Antigen conjugation	860:880	O-Antigen conjugation	860:880	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	4	29	theme	amino	800:804	arg1	acids					806:810	different amino acids	790:810	different amino acids on the carrier protein	790:833	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	3	30	used	used	629:632	arg2	O-Antigen					587:595	S. Typhimurium lipopolysaccharide O-Antigen	553:595	S. Typhimurium lipopolysaccharide O-Antigen	553:595	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	3	30	used	used	629:632	arg2	models					637:642	models	637:642	models	637:642	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	3	30	used	used	629:632	arg2	protein					616:622	CRM197 carrier protein	601:622	CRM197 carrier protein	601:622	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	1	31	theme	mechanism	304:312	arg1	understanding					281:293	a deeper understanding	272:293	a deeper understanding of their mechanism of action	272:322	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	0	32	theme	carrier	120:126	arg1	protein					128:134	carrier protein	120:134	carrier protein	120:134	Click chemistry compared to thiol chemistry for the synthesis of site-selective glycoconjugate vaccines using CRM197 as carrier protein.					
32535667	4	33	theme	different	790:798	arg1	acids					806:810	different amino acids	790:810	different amino acids on the carrier protein	790:833	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	5	34	from	position	1055:1062	arg1	protein					1071:1077	the protein	1067:1077	the protein	1067:1077	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	4	35	theme	terminal	735:742	arg1	unit					759:762	the terminal reducing sugar unit	731:762	the terminal reducing sugar unit of the O-Antigen chain	731:785	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	5	36	theme	high	961:964	arg1	number					966:971	a relative high number	950:971	a relative high number of linkers	950:982	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	5	37	theme	lysine	1105:1110	arg1	sites					1125:1129	both lysine and tyrosine sites	1100:1129	sites	1125:1129	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	1	38	theme	glycoconjugate	217:230	arg1	vaccines					232:239	glycoconjugate vaccines	217:239	glycoconjugate vaccines	217:239	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	0	39	theme	thiol	28:32	arg1	chemistry					34:42	thiol chemistry	28:42	thiol chemistry for the synthesis of site-selective glycoconjugate vaccines using CRM197 as carrier protein	28:134	Click chemistry compared to thiol chemistry for the synthesis of site-selective glycoconjugate vaccines using CRM197 as carrier protein.					
32535667	1	40	theme	action	317:322	arg1	mechanism					304:312	their mechanism	298:312	their mechanism of action	298:322	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	1	41	theme	vaccines	232:239	arg1	immunogenicity					199:212	immunogenicity	199:212	immunogenicity of glycoconjugate vaccines	199:239	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	2	42	theme	glycosylation	507:519	arg1	density					521:527	glycosylation density	507:527	glycosylation density	507:527	Herein, different conjugation methods were investigated with the aim of controlling glycosylation site and glycosylation density on the carrier protein.					
32535667	5	43	theme	tyrosine	1116:1123	arg1	sites					1125:1129	both lysine and tyrosine sites	1100:1129	sites	1125:1129	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	4	44	dep	examined	691:698	arg1	involving					706:714	involving	706:714	involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein	706:833	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	5	45	theme	relative	952:959	arg1	number					966:971	a relative high number	950:971	a relative high number of linkers	950:982	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	4	46	theme	unit	759:762	arg1	linkage					720:726	the linkage	716:726	the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein	716:833	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	2	47	gly	glycosylation	484:496	arg2	site					498:501	glycosylation site	484:501	glycosylation site	484:501	Herein, different conjugation methods were investigated with the aim of controlling glycosylation site and glycosylation density on the carrier protein.					
32535667	4	48	theme	O-Antigen	771:779	arg1	chain					781:785	the O-Antigen chain	767:785	the O-Antigen chain	767:785	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
32535667	6	49	theme	leading	1174:1180	arg1	approach					1182:1189	a leading approach	1172:1189	a leading approach for the synthesis of well-defined glycoconjugates	1172:1239	The study highlights click chemistry as a leading approach for the synthesis of well-defined glycoconjugates, useful to investigate the relationship between conjugate design and immune response.					
32535667	6	49	theme	leading	1174:1180	arg1	chemistry					1159:1167	click chemistry	1153:1167	click chemistry	1153:1167	The study highlights click chemistry as a leading approach for the synthesis of well-defined glycoconjugates, useful to investigate the relationship between conjugate design and immune response.					
32535667	3	50	theme	CRM197	601:606	arg1	O-Antigen					587:595	S. Typhimurium lipopolysaccharide O-Antigen	553:595	S. Typhimurium lipopolysaccharide O-Antigen	553:595	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	3	50	theme	CRM197	601:606	arg1	protein					616:622	CRM197 carrier protein	601:622	CRM197 carrier protein	601:622	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	3	50	theme	CRM197	601:606	arg1	models					637:642	models	637:642	models	637:642	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	6	51	theme	well-defined	1212:1223	arg1	glycoconjugates					1225:1239	well-defined glycoconjugates	1212:1239	well-defined glycoconjugates	1212:1239	The study highlights click chemistry as a leading approach for the synthesis of well-defined glycoconjugates, useful to investigate the relationship between conjugate design and immune response.					
32535667	1	52	theme	Conjugation	137:147	arg1	chemistry					149:157	Conjugation chemistry	137:157	Conjugation chemistry	137:157	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	6	53	theme	click	1153:1157	arg1	approach					1182:1189	a leading approach	1172:1189	a leading approach for the synthesis of well-defined glycoconjugates	1172:1239	The study highlights click chemistry as a leading approach for the synthesis of well-defined glycoconjugates, useful to investigate the relationship between conjugate design and immune response.					
32535667	6	53	theme	click	1153:1157	arg1	chemistry					1159:1167	click chemistry	1153:1167	click chemistry	1153:1167	The study highlights click chemistry as a leading approach for the synthesis of well-defined glycoconjugates, useful to investigate the relationship between conjugate design and immune response.					
32535667	5	54	theme	conjugate	1015:1023	arg1	generation					1025:1034	conjugate generation	1015:1034	conjugate generation	1015:1034	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	3	55	theme	lipopolysaccharide	568:585	arg1	protein					616:622	CRM197 carrier protein	601:622	CRM197 carrier protein	601:622	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	3	55	theme	lipopolysaccharide	568:585	arg1	models					637:642	models	637:642	models	637:642	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	3	55	theme	lipopolysaccharide	568:585	arg1	O-Antigen					587:595	S. Typhimurium lipopolysaccharide O-Antigen	553:595	S. Typhimurium lipopolysaccharide O-Antigen	553:595	S. Typhimurium lipopolysaccharide O-Antigen and CRM197 carrier protein were used as models.					
32535667	1	56	theme	rational	247:254	arg1	approach					256:263	a rational approach	245:263	a rational approach toward a deeper understanding of their mechanism of action	245:322	Conjugation chemistry is one of the main parameters affecting immunogenicity of glycoconjugate vaccines and a rational approach toward a deeper understanding of their mechanism of action will greatly benefit from highly-defined and well-characterized structures.					
32535667	5	57	theme	Thiol	836:840	arg1	chemistry					842:850	Thiol chemistry	836:850	Thiol chemistry	836:850	Thiol chemistry allowed O-Antigen conjugation only when the carrier protein was activated on the lysines and with a relative high number of linkers, while click chemistry allowed conjugate generation even when just one position on the protein was activated and to both lysine and tyrosine sites.					
32535667	6	58	theme	glycoconjugates	1225:1239	arg1	synthesis					1199:1207	the synthesis	1195:1207	the synthesis of well-defined glycoconjugates	1195:1239	The study highlights click chemistry as a leading approach for the synthesis of well-defined glycoconjugates, useful to investigate the relationship between conjugate design and immune response.					
32535667	4	59	theme	click	670:674	arg1	chemistry					676:684	click chemistry	670:684	click chemistry	670:684	In particular, thiol and click chemistry were examined, both involving the linkage of the terminal reducing sugar unit of the O-Antigen chain to different amino acids on the carrier protein.					
33010307	8	0	theme	protein	1132:1138	arg1	spectra					1117:1123	the NMR spectra	1109:1123	the NMR spectra of the protein	1109:1138	The two sets of cross peaks in the NMR spectra of the protein supported the conformational changes between the two states in detergent-solubilized conditions.					
33010307	0	1	from	Dynamics	19:26	arg1	Oligosaccharyltransferase					49:73	Membrane-Embedded Oligosaccharyltransferase	31:73	Membrane-Embedded Oligosaccharyltransferase	31:73	Two-State Exchange Dynamics in Membrane-Embedded Oligosaccharyltransferase Observed in Real-Time by High-Speed AFM.					
33010307	12	2	gly	glycosylated	1697:1708	arg1	product					1722:1728	the bulky glycosylated polypeptide product	1687:1728	the bulky glycosylated polypeptide product	1687:1728	Presumably, state 2 facilitates the immediate release of the bulky glycosylated polypeptide product, thus allowing OST to quickly prepare for the next catalytic cycle.					
33010307	11	3	theme	efficient	1600:1608	arg1	turnover					1620:1627	efficient catalytic turnover	1600:1627	efficient catalytic turnover	1600:1627	This dynamic behavior is likely necessary for efficient catalytic turnover.					
33010307	2	4	theme	dynamic	384:390	arg1	properties					392:401	the dynamic properties	380:401	the dynamic properties of OST molecules embedded in biomembranes	380:443	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	0	5	theme	High-Speed	100:109	arg1	AFM					111:113	High-Speed AFM	100:113	High-Speed AFM	100:113	Two-State Exchange Dynamics in Membrane-Embedded Oligosaccharyltransferase Observed in Real-Time by High-Speed AFM.					
33010307	9	6	contain	has	1357:1359	arg1	state					1349:1353	state 2	1349:1355	state 2	1349:1355	Considering the height values measured in the AFM measurements, state 1 is closer to the crystal structure, and state 2 has a more compact form.					
33010307	9	6	contain	has	1357:1359	arg2	form					1376:1379	a more compact form	1361:1379	a more compact form	1361:1379	Considering the height values measured in the AFM measurements, state 1 is closer to the crystal structure, and state 2 has a more compact form.					
33010307	3	7	theme	single-subunit	458:471	arg1	protein					477:483	An archaeal single-subunit OST protein	446:483	An archaeal single-subunit OST protein	446:483	An archaeal single-subunit OST protein was immobilized on a mica support via biotin-avidin interactions and reconstituted in a lipid bilayer.					
33010307	12	8	theme	bulky	1691:1695	arg1	product					1722:1728	the bulky glycosylated polypeptide product	1687:1728	the bulky glycosylated polypeptide product	1687:1728	Presumably, state 2 facilitates the immediate release of the bulky glycosylated polypeptide product, thus allowing OST to quickly prepare for the next catalytic cycle.					
33010307	7	9	theme	single	948:953	arg1	functions					967:975	single exponential functions	948:975	single exponential functions	948:975	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	2	10	theme	atomic	333:338	arg1	microscopy					346:355	high-speed atomic force microscopy	322:355	high-speed atomic force microscopy (AFM)	322:361	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	2	10	theme	atomic	333:338	arg1	AFM					358:360	AFM	358:360	AFM	358:360	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	9	11	theme	crystal	1326:1332	arg1	structure					1334:1342	the crystal structure	1322:1342	the crystal structure	1322:1342	Considering the height values measured in the AFM measurements, state 1 is closer to the crystal structure, and state 2 has a more compact form.					
33010307	1	12	theme	polypeptide	296:306	arg1	chains					308:313	polypeptide chains	296:313	polypeptide chains	296:313	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	5	13	theme	two-step	763:770	arg1	variation					772:780	a two-step variation	761:780	a two-step variation	761:780	The height of the extramembranous part exhibited a two-step variation with a difference of 1.8 nm.					
33010307	1	14	link	lipid-linked	234:245	arg1	LLO					265:267	LLO	265:267	LLO	265:267	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	1	14	link	lipid-linked	234:245	arg1	oligosaccharides					247:262	lipid-linked oligosaccharides	234:262	lipid-linked oligosaccharides (LLO)	234:268	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	7	15	theme	exponential	955:965	arg1	functions					967:975	single exponential functions	948:975	single exponential functions	948:975	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	2	16	theme	high-speed	322:331	arg1	microscopy					346:355	high-speed atomic force microscopy	322:355	high-speed atomic force microscopy (AFM)	322:361	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	2	16	theme	high-speed	322:331	arg1	AFM					358:360	AFM	358:360	AFM	358:360	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	4	17	theme	protein	624:630	arg1	molecule					632:639	the protein molecule	620:639	the protein molecule	620:639	The distance between the top of the protein molecule and the upper surface of the lipid bilayer was monitored in real-time.					
33010307	10	18	theme	LLO	1455:1457	arg1	binding					1428:1434	the binding	1424:1434	the binding of the sugar donor LLO	1424:1457	Subsequent AFM experiments indicated that the binding of the sugar donor LLO decreased the structural fluctuation and shifted the equilibrium almost completely to state 1.					
33010307	3	19	theme	mica	506:509	arg1	support					511:517	a mica support	504:517	a mica support	504:517	An archaeal single-subunit OST protein was immobilized on a mica support via biotin-avidin interactions and reconstituted in a lipid bilayer.					
33010307	3	20	theme	lipid	573:577	arg1	bilayer					579:585	a lipid bilayer	571:585	a lipid bilayer	571:585	An archaeal single-subunit OST protein was immobilized on a mica support via biotin-avidin interactions and reconstituted in a lipid bilayer.					
33010307	2	21	theme	molecules	410:418	arg1	properties					392:401	the dynamic properties	380:401	the dynamic properties of OST molecules embedded in biomembranes	380:443	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	8	22	theme	conformational	1154:1167	arg1	changes					1169:1175	the conformational changes	1150:1175	the conformational changes between the two states in detergent-solubilized conditions	1150:1234	The two sets of cross peaks in the NMR spectra of the protein supported the conformational changes between the two states in detergent-solubilized conditions.					
33010307	12	23	theme	next	1776:1779	arg1	cycle					1791:1795	the next catalytic cycle	1772:1795	the next catalytic cycle	1772:1795	Presumably, state 2 facilitates the immediate release of the bulky glycosylated polypeptide product, thus allowing OST to quickly prepare for the next catalytic cycle.					
33010307	3	24	theme	OST	473:475	arg1	protein					477:483	An archaeal single-subunit OST protein	446:483	An archaeal single-subunit OST protein	446:483	An archaeal single-subunit OST protein was immobilized on a mica support via biotin-avidin interactions and reconstituted in a lipid bilayer.					
33010307	8	25	theme	cross	1094:1098	arg1	peaks					1100:1104	cross peaks	1094:1104	cross peaks	1094:1104	The two sets of cross peaks in the NMR spectra of the protein supported the conformational changes between the two states in detergent-solubilized conditions.					
33010307	2	26	theme	embedded	420:427	arg1	molecules					410:418	OST molecules	406:418	OST molecules embedded in biomembranes	406:443	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	0	27	theme	Exchange	10:17	arg1	Dynamics					19:26	Two-State Exchange Dynamics	0:26	Two-State Exchange Dynamics in Membrane-Embedded Oligosaccharyltransferase	0:73	Two-State Exchange Dynamics in Membrane-Embedded Oligosaccharyltransferase Observed in Real-Time by High-Speed AFM.					
33010307	5	28	theme	1.8 nm	803:808	arg1	difference					789:798	a difference	787:798	a difference of 1.8 nm	787:808	The height of the extramembranous part exhibited a two-step variation with a difference of 1.8 nm.					
33010307	10	29	theme	sugar	1443:1447	arg1	LLO					1455:1457	the sugar donor LLO	1439:1457	the sugar donor LLO	1439:1457	Subsequent AFM experiments indicated that the binding of the sugar donor LLO decreased the structural fluctuation and shifted the equilibrium almost completely to state 1.					
33010307	6	30	theme	high	815:818	arg1	states					828:833	The high and low states	811:833	The high and low states	811:833	The high and low states are designated as state 1 and state 2, respectively.					
33010307	6	30	theme	high	815:818	arg1	state					853:857	state 1	853:859	state 1	853:859	The high and low states are designated as state 1 and state 2, respectively.					
33010307	6	30	theme	high	815:818	arg1	state					865:869	state 2	865:871	state 2	865:871	The high and low states are designated as state 1 and state 2, respectively.					
33010307	8	31	theme	peaks	1100:1104	arg1	sets					1086:1089	The two sets	1078:1089	The two sets of cross peaks in the NMR spectra of the protein	1078:1138	The two sets of cross peaks in the NMR spectra of the protein supported the conformational changes between the two states in detergent-solubilized conditions.					
33010307	8	31	theme	peaks	1100:1104	arg1	peaks					1100:1104	cross peaks	1094:1104	cross peaks	1094:1104	The two sets of cross peaks in the NMR spectra of the protein supported the conformational changes between the two states in detergent-solubilized conditions.					
33010307	1	32	theme	oligosaccharide	206:220	arg1	chains					222:227	oligosaccharide chains	206:227	oligosaccharide chains	206:227	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	0	33	theme	Two-State	0:8	arg1	Dynamics					19:26	Two-State Exchange Dynamics	0:26	Two-State Exchange Dynamics in Membrane-Embedded Oligosaccharyltransferase	0:73	Two-State Exchange Dynamics in Membrane-Embedded Oligosaccharyltransferase Observed in Real-Time by High-Speed AFM.					
33010307	5	34	theme	part	746:749	arg1	height					716:721	The height	712:721	The height of the extramembranous part	712:749	The height of the extramembranous part exhibited a two-step variation with a difference of 1.8 nm.					
33010307	10	35	theme	AFM	1393:1395	arg1	experiments					1397:1407	Subsequent AFM experiments	1382:1407	Subsequent AFM experiments	1382:1407	Subsequent AFM experiments indicated that the binding of the sugar donor LLO decreased the structural fluctuation and shifted the equilibrium almost completely to state 1.					
33010307	11	36	theme	dynamic	1559:1565	arg1	behavior					1567:1574	This dynamic behavior	1554:1574	This dynamic behavior	1554:1574	This dynamic behavior is likely necessary for efficient catalytic turnover.					
33010307	1	37	theme	chains	222:227	arg1	transfer					194:201	the transfer	190:201	the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains	190:313	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	6	38	theme	low	824:826	arg1	states					828:833	The high and low states	811:833	The high and low states	811:833	The high and low states are designated as state 1 and state 2, respectively.					
33010307	6	38	theme	low	824:826	arg1	state					853:857	state 1	853:859	state 1	853:859	The high and low states are designated as state 1 and state 2, respectively.					
33010307	6	38	theme	low	824:826	arg1	state					865:869	state 2	865:871	state 2	865:871	The high and low states are designated as state 1 and state 2, respectively.					
33010307	7	39	theme	dynamic	1007:1013	arg1	property					1040:1047	an intrinsic property	1027:1047	an intrinsic property of the archaeal OST protein	1027:1075	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	7	39	theme	dynamic	1007:1013	arg1	exchange					1015:1022	the observed dynamic exchange	994:1022	the observed dynamic exchange	994:1022	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	7	40	theme	OST	1065:1067	arg1	protein					1069:1075	the archaeal OST protein	1052:1075	the archaeal OST protein	1052:1075	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	5	41	theme	extramembranous	730:744	arg1	part					746:749	the extramembranous part	726:749	the extramembranous part	726:749	The height of the extramembranous part exhibited a two-step variation with a difference of 1.8 nm.					
33010307	2	42	theme	force	340:344	arg1	microscopy					346:355	high-speed atomic force microscopy	322:355	high-speed atomic force microscopy (AFM)	322:361	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	2	42	theme	force	340:344	arg1	AFM					358:360	AFM	358:360	AFM	358:360	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	1	43	theme	lipid-linked	234:245	arg1	LLO					265:267	LLO	265:267	LLO	265:267	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	1	43	theme	lipid-linked	234:245	arg1	oligosaccharides					247:262	lipid-linked oligosaccharides	234:262	lipid-linked oligosaccharides (LLO)	234:268	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	8	44	theme	detergent-solubilized	1203:1223	arg1	conditions					1225:1234	detergent-solubilized conditions	1203:1234	detergent-solubilized conditions	1203:1234	The two sets of cross peaks in the NMR spectra of the protein supported the conformational changes between the two states in detergent-solubilized conditions.					
33010307	1	45	from	residues	284:291	arg1	chains					308:313	polypeptide chains	296:313	polypeptide chains	296:313	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	11	46	theme	catalytic	1610:1618	arg1	turnover					1620:1627	efficient catalytic turnover	1600:1627	efficient catalytic turnover	1600:1627	This dynamic behavior is likely necessary for efficient catalytic turnover.					
33010307	2	47	theme	OST	406:408	arg1	molecules					410:418	OST molecules	406:418	OST molecules embedded in biomembranes	406:443	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	4	48	theme	bilayer	676:682	arg1	surface					655:661	the upper surface	645:661	the upper surface of the lipid bilayer	645:682	The distance between the top of the protein molecule and the upper surface of the lipid bilayer was monitored in real-time.					
33010307	4	48	theme	bilayer	676:682	arg1	top					613:615	the top	609:615	the top of the protein molecule	609:639	The distance between the top of the protein molecule and the upper surface of the lipid bilayer was monitored in real-time.					
33010307	0	49	theme	Membrane-Embedded	31:47	arg1	Oligosaccharyltransferase					49:73	Membrane-Embedded Oligosaccharyltransferase	31:73	Membrane-Embedded Oligosaccharyltransferase	31:73	Two-State Exchange Dynamics in Membrane-Embedded Oligosaccharyltransferase Observed in Real-Time by High-Speed AFM.					
33010307	3	50	theme	archaeal	449:456	arg1	protein					477:483	An archaeal single-subunit OST protein	446:483	An archaeal single-subunit OST protein	446:483	An archaeal single-subunit OST protein was immobilized on a mica support via biotin-avidin interactions and reconstituted in a lipid bilayer.					
33010307	12	51	theme	catalytic	1781:1789	arg1	cycle					1791:1795	the next catalytic cycle	1772:1795	the next catalytic cycle	1772:1795	Presumably, state 2 facilitates the immediate release of the bulky glycosylated polypeptide product, thus allowing OST to quickly prepare for the next catalytic cycle.					
33010307	1	52	from	oligosaccharides	247:262	arg1	transfer					194:201	the transfer	190:201	the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains	190:313	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	4	53	theme	lipid	670:674	arg1	bilayer					676:682	the lipid bilayer	666:682	the lipid bilayer	666:682	The distance between the top of the protein molecule and the upper surface of the lipid bilayer was monitored in real-time.					
33010307	7	54	theme	intrinsic	1030:1038	arg1	property					1040:1047	an intrinsic property	1027:1047	an intrinsic property of the archaeal OST protein	1027:1075	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	7	54	theme	intrinsic	1030:1038	arg1	exchange					1015:1022	the observed dynamic exchange	994:1022	the observed dynamic exchange	994:1022	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	3	55	theme	biotin-avidin	523:535	arg1	interactions					537:548	biotin-avidin interactions	523:548	biotin-avidin interactions	523:548	An archaeal single-subunit OST protein was immobilized on a mica support via biotin-avidin interactions and reconstituted in a lipid bilayer.					
33010307	7	56	theme	protein	1069:1075	arg1	property					1040:1047	an intrinsic property	1027:1047	an intrinsic property of the archaeal OST protein	1027:1075	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	7	56	theme	protein	1069:1075	arg1	exchange					1015:1022	the observed dynamic exchange	994:1022	the observed dynamic exchange	994:1022	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	7	57	theme	archaeal	1056:1063	arg1	protein					1069:1075	the archaeal OST protein	1052:1075	the archaeal OST protein	1052:1075	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	8	58	from	changes	1169:1175	arg1	conditions					1225:1234	detergent-solubilized conditions	1203:1234	detergent-solubilized conditions	1203:1234	The two sets of cross peaks in the NMR spectra of the protein supported the conformational changes between the two states in detergent-solubilized conditions.					
33010307	10	59	theme	donor	1449:1453	arg1	LLO					1455:1457	the sugar donor LLO	1439:1457	the sugar donor LLO	1439:1457	Subsequent AFM experiments indicated that the binding of the sugar donor LLO decreased the structural fluctuation and shifted the equilibrium almost completely to state 1.					
33010307	12	60	theme	polypeptide	1710:1720	arg1	product					1722:1728	the bulky glycosylated polypeptide product	1687:1728	the bulky glycosylated polypeptide product	1687:1728	Presumably, state 2 facilitates the immediate release of the bulky glycosylated polypeptide product, thus allowing OST to quickly prepare for the next catalytic cycle.					
33010307	10	61	theme	Subsequent	1382:1391	arg1	experiments					1397:1407	Subsequent AFM experiments	1382:1407	Subsequent AFM experiments	1382:1407	Subsequent AFM experiments indicated that the binding of the sugar donor LLO decreased the structural fluctuation and shifted the equilibrium almost completely to state 1.					
33010307	8	62	theme	NMR	1113:1115	arg1	spectra					1117:1123	the NMR spectra	1109:1123	the NMR spectra of the protein	1109:1138	The two sets of cross peaks in the NMR spectra of the protein supported the conformational changes between the two states in detergent-solubilized conditions.					
33010307	2	63	from	embedded	420:427	arg1	biomembranes					432:443	biomembranes	432:443	biomembranes	432:443	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	12	64	theme	glycosylated	1697:1708	arg1	product					1722:1728	the bulky glycosylated polypeptide product	1687:1728	the bulky glycosylated polypeptide product	1687:1728	Presumably, state 2 facilitates the immediate release of the bulky glycosylated polypeptide product, thus allowing OST to quickly prepare for the next catalytic cycle.					
33010307	12	65	theme	immediate	1666:1674	arg1	release					1676:1682	the immediate release	1662:1682	the immediate release of the bulky glycosylated polypeptide product	1662:1728	Presumably, state 2 facilitates the immediate release of the bulky glycosylated polypeptide product, thus allowing OST to quickly prepare for the next catalytic cycle.					
33010307	7	66	theme	observed	998:1005	arg1	property					1040:1047	an intrinsic property	1027:1047	an intrinsic property of the archaeal OST protein	1027:1075	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	7	66	theme	observed	998:1005	arg1	exchange					1015:1022	the observed dynamic exchange	994:1022	the observed dynamic exchange	994:1022	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	1	67	theme	membrane-bound	153:166	arg1	Oligosaccharyltransferase					116:140	Oligosaccharyltransferase	116:140	Oligosaccharyltransferase (OST)	116:146	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	1	67	theme	membrane-bound	153:166	arg1	enzyme					168:173	a membrane-bound enzyme	151:173	a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains	151:313	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
33010307	9	68	theme	compact	1368:1374	arg1	form					1376:1379	a more compact form	1361:1379	a more compact form	1361:1379	Considering the height values measured in the AFM measurements, state 1 is closer to the crystal structure, and state 2 has a more compact form.					
33010307	10	69	theme	structural	1473:1482	arg1	fluctuation					1484:1494	the structural fluctuation	1469:1494	the structural fluctuation	1469:1494	Subsequent AFM experiments indicated that the binding of the sugar donor LLO decreased the structural fluctuation and shifted the equilibrium almost completely to state 1.					
33010307	9	70	theme	AFM	1283:1285	arg1	measurements					1287:1298	the AFM measurements	1279:1298	the AFM measurements	1279:1298	Considering the height values measured in the AFM measurements, state 1 is closer to the crystal structure, and state 2 has a more compact form.					
33010307	2	71	from	biomembranes	432:443	arg1	embedded					420:427	embedded	420:427	embedded	420:427	Using high-speed atomic force microscopy (AFM), we investigated the dynamic properties of OST molecules embedded in biomembranes.					
33010307	12	72	theme	product	1722:1728	arg1	release					1676:1682	the immediate release	1662:1682	the immediate release of the bulky glycosylated polypeptide product	1662:1728	Presumably, state 2 facilitates the immediate release of the bulky glycosylated polypeptide product, thus allowing OST to quickly prepare for the next catalytic cycle.					
33010307	8	73	from	sets	1086:1089	arg1	spectra					1117:1123	the NMR spectra	1109:1123	the NMR spectra of the protein	1109:1138	The two sets of cross peaks in the NMR spectra of the protein supported the conformational changes between the two states in detergent-solubilized conditions.					
33010307	4	74	theme	molecule	632:639	arg1	surface					655:661	the upper surface	645:661	the upper surface of the lipid bilayer	645:682	The distance between the top of the protein molecule and the upper surface of the lipid bilayer was monitored in real-time.					
33010307	4	74	theme	molecule	632:639	arg1	top					613:615	the top	609:615	the top of the protein molecule	609:639	The distance between the top of the protein molecule and the upper surface of the lipid bilayer was monitored in real-time.					
33010307	4	75	theme	upper	649:653	arg1	surface					655:661	the upper surface	645:661	the upper surface of the lipid bilayer	645:682	The distance between the top of the protein molecule and the upper surface of the lipid bilayer was monitored in real-time.					
33010307	7	76	theme	transition	892:901	arg1	processes					903:911	The transition processes	888:911	The transition processes between the two states	888:934	The transition processes between the two states fit well to single exponential functions, suggesting that the observed dynamic exchange is an intrinsic property of the archaeal OST protein.					
33010307	1	77	theme	asparagine	273:282	arg1	residues					284:291	asparagine residues	273:291	asparagine residues in polypeptide chains	273:313	Oligosaccharyltransferase (OST) is a membrane-bound enzyme that catalyzes the transfer of oligosaccharide chains from lipid-linked oligosaccharides (LLO) to asparagine residues in polypeptide chains.					
31729663	3	0	theme	structural	528:537	arg1	assignments					539:549	structural assignments	528:549	structural assignments of individual glycans	528:571	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	2	1	from	challenge	355:363	arg1	biology					379:385	structural biology	368:385	structural biology	368:385	Their biosynthesis is nontemplate-driven, achieved stepwise in discrete locations within the cell, giving rise to a range of complex branched structures that pose a significant challenge in structural biology.					
31729663	3	2	theme	Mass	388:391	arg1	method					421:426	the leading method	409:426	the leading method for analysis of glycoconjugates	409:458	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	3	2	theme	Mass	388:391	arg1	spectrometry					393:404	Mass spectrometry	388:404	Mass spectrometry	388:404	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	1	3	theme	diverse	71:77	arg1	Glycoconjugates					51:65	Glycoconjugates	51:65	Glycoconjugates	51:65	Glycoconjugates are diverse biomolecules that are dynamically assembled to regulate and fine-tune numerous cellular processes.					
31729663	1	3	theme	diverse	71:77	arg1	biomolecules					79:90	diverse biomolecules	71:90	diverse biomolecules that are dynamically assembled to regulate and fine-tune numerous cellular processes	71:175	Glycoconjugates are diverse biomolecules that are dynamically assembled to regulate and fine-tune numerous cellular processes.					
31729663	2	4	theme	complex	303:309	arg1	structures					320:329	complex branched structures	303:329	complex branched structures that pose a significant challenge in structural biology	303:385	Their biosynthesis is nontemplate-driven, achieved stepwise in discrete locations within the cell, giving rise to a range of complex branched structures that pose a significant challenge in structural biology.					
31729663	3	5	theme	biological	584:593	arg1	mixtures					595:602	complex biological mixtures	576:602	complex biological mixtures	576:602	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	2	6	theme	significant	343:353	arg1	challenge					355:363	a significant challenge	341:363	a significant challenge in structural biology	341:385	Their biosynthesis is nontemplate-driven, achieved stepwise in discrete locations within the cell, giving rise to a range of complex branched structures that pose a significant challenge in structural biology.					
31729663	4	7	theme	IM-MS	733:737	arg1	data					739:742	IM-MS data	733:742	IM-MS data for the analysis of glycans	733:770	In this chapter, we briefly discuss recent applications of IM for glycomics and describe how to acquire, interpret, and analyze IM-MS data for the analysis of glycans.					
31729663	3	8	theme	glycans	565:571	arg1	assignments					539:549	structural assignments	528:549	structural assignments of individual glycans	528:571	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	0	9	theme	Mobility-Mass	4:16	arg1	Spectrometry					18:29	Ion Mobility-Mass Spectrometry	0:29	Ion Mobility-Mass Spectrometry of Glycoconjugates	0:48	Ion Mobility-Mass Spectrometry of Glycoconjugates.					
31729663	3	10	theme	individual	554:563	arg1	glycans					565:571	individual glycans	554:571	individual glycans	554:571	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	0	11	theme	Ion	0:2	arg1	Spectrometry					18:29	Ion Mobility-Mass Spectrometry	0:29	Ion Mobility-Mass Spectrometry of Glycoconjugates	0:48	Ion Mobility-Mass Spectrometry of Glycoconjugates.					
31729663	4	12	theme	glycans	764:770	arg1	analysis					752:759	the analysis	748:759	the analysis of glycans	748:770	In this chapter, we briefly discuss recent applications of IM for glycomics and describe how to acquire, interpret, and analyze IM-MS data for the analysis of glycans.					
31729663	3	13	theme	mobility	485:492	arg1	addition					469:476	the addition	465:476	the addition of ion mobility	465:492	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	2	14	theme	structures	320:329	arg1	range					294:298	a range	292:298	a range of complex branched structures that pose a significant challenge in structural biology	292:385	Their biosynthesis is nontemplate-driven, achieved stepwise in discrete locations within the cell, giving rise to a range of complex branched structures that pose a significant challenge in structural biology.					
31729663	4	15	theme	IM	664:665	arg1	applications					648:659	recent applications	641:659	recent applications of IM for glycomics	641:679	In this chapter, we briefly discuss recent applications of IM for glycomics and describe how to acquire, interpret, and analyze IM-MS data for the analysis of glycans.					
31729663	0	16	theme	Glycoconjugates	34:48	arg1	Spectrometry					18:29	Ion Mobility-Mass Spectrometry	0:29	Ion Mobility-Mass Spectrometry of Glycoconjugates	0:48	Ion Mobility-Mass Spectrometry of Glycoconjugates.					
31729663	3	17	theme	glycoconjugates	444:458	arg1	analysis					432:439	analysis	432:439	analysis of glycoconjugates	432:458	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	3	18	theme	ion	481:483	arg1	mobility					485:492	ion mobility	481:492	ion mobility	481:492	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	2	19	theme	structural	368:377	arg1	biology					379:385	structural biology	368:385	structural biology	368:385	Their biosynthesis is nontemplate-driven, achieved stepwise in discrete locations within the cell, giving rise to a range of complex branched structures that pose a significant challenge in structural biology.					
31729663	2	20	theme	branched	311:318	arg1	structures					320:329	complex branched structures	303:329	complex branched structures that pose a significant challenge in structural biology	303:385	Their biosynthesis is nontemplate-driven, achieved stepwise in discrete locations within the cell, giving rise to a range of complex branched structures that pose a significant challenge in structural biology.					
31729663	1	21	theme	fine-tune	139:147	arg1	processes					167:175	and fine-tune numerous cellular processes	135:175	processes	167:175	Glycoconjugates are diverse biomolecules that are dynamically assembled to regulate and fine-tune numerous cellular processes.					
31729663	2	22	theme	discrete	241:248	arg1	locations					250:258	discrete locations	241:258	discrete locations within the cell	241:274	Their biosynthesis is nontemplate-driven, achieved stepwise in discrete locations within the cell, giving rise to a range of complex branched structures that pose a significant challenge in structural biology.					
31729663	1	23	theme	numerous	149:156	arg1	processes					167:175	and fine-tune numerous cellular processes	135:175	processes	167:175	Glycoconjugates are diverse biomolecules that are dynamically assembled to regulate and fine-tune numerous cellular processes.					
31729663	4	24	theme	recent	641:646	arg1	applications					648:659	recent applications	641:659	recent applications of IM for glycomics	641:679	In this chapter, we briefly discuss recent applications of IM for glycomics and describe how to acquire, interpret, and analyze IM-MS data for the analysis of glycans.					
31729663	3	25	theme	complex	576:582	arg1	mixtures					595:602	complex biological mixtures	576:602	complex biological mixtures	576:602	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	1	26	theme	cellular	158:165	arg1	processes					167:175	and fine-tune numerous cellular processes	135:175	processes	167:175	Glycoconjugates are diverse biomolecules that are dynamically assembled to regulate and fine-tune numerous cellular processes.					
31729663	3	27	theme	leading	413:419	arg1	method					421:426	the leading method	409:426	the leading method for analysis of glycoconjugates	409:458	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
31729663	3	27	theme	leading	413:419	arg1	spectrometry					393:404	Mass spectrometry	388:404	Mass spectrometry	388:404	Mass spectrometry is the leading method for analysis of glycoconjugates, and the addition of ion mobility has proven valuable for improving structural assignments of individual glycans in complex biological mixtures.					
32898175	7	0	theme	spleen	1510:1515	arg1	cells					1517:1521	spleen cells	1510:1521	spleen cells	1510:1521	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	10	1	theme	biologicals	2101:2111	arg1	discovery					2041:2049	the discovery	2037:2049	the discovery of future parasite vaccines and immunotherapeutic biologicals	2037:2111	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	9	2	theme	capable	1891:1897	arg1	DC					1888:1889	DC	1888:1889	DC capable of suppressing IL-2 secretion from T-cells	1888:1940	Thus, we have demonstrated that FhEVs induce a unique phentotype in DC capable of suppressing IL-2 secretion from T-cells.					
32898175	6	3	link	derived	1115:1121	arg1	cells					1133:1137	bone-marrow derived dendritic cells	1103:1137	bone-marrow derived dendritic cells isolated FhEV	1103:1151	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	3	4	theme	FhEVs	554:558	arg1	cargo					570:574	The FhEVs molecular cargo	550:574	The FhEVs molecular cargo	550:574	The FhEVs molecular cargo was defined using proteomic analysis and their surface topology characterised by glycan microarrays.					
32898175	1	5	theme	long-term	286:294	arg1	relationship					296:307	the long-term relationship	282:307	the long-term relationship between parasite and host	282:333	Parasite-released extracellular vesicles (EVs) deliver signals to the host immune system that are critical to maintaining the long-term relationship between parasite and host.					
32898175	7	6	theme	draining	1485:1492	arg1	nodes					1500:1504	skin draining lymph nodes	1480:1504	skin draining lymph nodes	1480:1504	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	7	7	theme	adoptive	1441:1448	arg1	transfer					1450:1457	adoptive transfer	1441:1457	adoptive transfer	1441:1457	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	4	8	theme	proteomic	681:689	arg1	analysis					691:698	The proteomic analysis	677:698	The proteomic analysis	677:698	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	6	9	theme	low	1220:1222	arg1	levels					1224:1229	low levels	1220:1229	low levels of TNF	1220:1236	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	10	theme	novel	1163:1167	arg1	phenotype					1169:1177	a novel phenotype	1161:1177	a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3)	1161:1376	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	8	11	theme	mixed	1746:1750	arg1	response					1767:1774	a mixed Th1/Th2 immune response	1744:1774	a mixed Th1/Th2 immune response	1744:1774	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	4	12	contain	contained	805:813	arg1	proteins					796:803	132 proteins	792:803	132 proteins	792:803	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	4	12	contain	contained	805:813	arg2	sites					847:851	putative O-linked glycosylation sites	815:851	putative O-linked glycosylation sites	815:851	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	6	13	theme	markers	1352:1358	arg1	expression					1248:1257	enhanced expression	1239:1257	enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1)	1239:1319	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	13	theme	markers	1352:1358	arg1	elevation					1325:1333	elevation	1325:1333	elevation of intracellular markers (SOCS1 and SOCS3)	1325:1376	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	13	theme	markers	1352:1358	arg1	secretion					1207:1215	the secretion	1203:1215	the secretion of low levels of TNF	1203:1236	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	8	14	theme	immune	1760:1765	arg1	response					1767:1774	a mixed Th1/Th2 immune response	1744:1774	a mixed Th1/Th2 immune response	1744:1774	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	5	15	theme	high	908:911	arg1	affinity					913:920	a high affinity	906:920	a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins	906:1012	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	7	16	theme	FhEV-stimulated	1384:1398	arg1	BMDCs					1400:1404	FhEV-stimulated BMDCs	1384:1404	FhEV-stimulated BMDCs	1384:1404	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	10	17	theme	immuno-proteomic	1974:1989	arg1	database					1991:1998	the growing immuno-proteomic database	1962:1998	the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals	1962:2111	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	10	17	theme	immuno-proteomic	1974:1989	arg1	source					2026:2031	an important source	2013:2031	an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals	2013:2111	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	3	18	theme	proteomic	594:602	arg1	analysis					604:611	proteomic analysis	594:611	proteomic analysis	594:611	The FhEVs molecular cargo was defined using proteomic analysis and their surface topology characterised by glycan microarrays.					
32898175	5	19	theme	mannose-rich	987:998	arg1	glycoproteins					1000:1012	oligo mannose-rich glycoproteins	981:1012	oligo mannose-rich glycoproteins	981:1012	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	6	20	theme	markers	1275:1281	arg1	expression					1248:1257	enhanced expression	1239:1257	enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1)	1239:1319	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	20	theme	markers	1275:1281	arg1	elevation					1325:1333	elevation	1325:1333	elevation of intracellular markers (SOCS1 and SOCS3)	1325:1376	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	20	theme	markers	1275:1281	arg1	secretion					1207:1215	the secretion	1203:1215	the secretion of low levels of TNF	1203:1236	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	21	dep	markers	1275:1281	arg1	markers					1275:1281	cell surface markers	1262:1281	cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1)	1262:1319	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	21	dep	markers	1275:1281	arg1	CD40					1296:1299	CD40	1296:1299	CD40	1296:1299	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	21	dep	markers	1275:1281	arg1	OX40L					1302:1306	OX40L	1302:1306	OX40L	1302:1306	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	21	dep	markers	1275:1281	arg1	CD80					1284:1287	CD80	1284:1287	CD80	1284:1287	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	21	dep	markers	1275:1281	arg1	CD86					1290:1293	CD86	1290:1293	CD86	1290:1293	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	21	dep	markers	1275:1281	arg1	SIGNR1					1313:1318	SIGNR1	1313:1318	SIGNR1	1313:1318	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	10	22	theme	important	2016:2024	arg1	database					1991:1998	the growing immuno-proteomic database	1962:1998	the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals	1962:2111	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	10	22	theme	important	2016:2024	arg1	source					2026:2031	an important source	2013:2031	an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals	2013:2111	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	8	23	theme	antigen	1786:1792	arg1	titres					1812:1817	high antigen specific antibody titres	1781:1817	high antigen specific antibody titres	1781:1817	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	4	24	theme	putative	748:755	arg1	sites					780:784	putative N-linked glycosylation sites	748:784	putative N-linked glycosylation sites	748:784	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	6	25	theme	cell	1262:1265	arg1	markers					1275:1281	cell surface markers	1262:1281	cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1)	1262:1319	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	25	theme	cell	1262:1265	arg1	CD40					1296:1299	CD40	1296:1299	CD40	1296:1299	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	25	theme	cell	1262:1265	arg1	OX40L					1302:1306	OX40L	1302:1306	OX40L	1302:1306	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	25	theme	cell	1262:1265	arg1	CD80					1284:1287	CD80	1284:1287	CD80	1284:1287	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	25	theme	cell	1262:1265	arg1	CD86					1290:1293	CD86	1290:1293	CD86	1290:1293	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	25	theme	cell	1262:1265	arg1	SIGNR1					1313:1318	SIGNR1	1313:1318	SIGNR1	1313:1318	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	4	26	theme	proteins	715:722	arg1	121					725:727	121	725:727	121	725:727	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	4	26	theme	proteins	715:722	arg1	proteins					715:722	618 proteins	711:722	618 proteins	711:722	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	3	27	theme	surface	623:629	arg1	topology					631:638	their surface topology	617:638	their surface topology	617:638	The FhEVs molecular cargo was defined using proteomic analysis and their surface topology characterised by glycan microarrays.					
32898175	8	28	theme	antibody	1803:1810	arg1	titres					1812:1817	high antigen specific antibody titres	1781:1817	high antigen specific antibody titres	1781:1817	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	1	29	theme	extracellular	178:190	arg1	EVs					202:204	EVs	202:204	EVs	202:204	Parasite-released extracellular vesicles (EVs) deliver signals to the host immune system that are critical to maintaining the long-term relationship between parasite and host.					
32898175	1	29	theme	extracellular	178:190	arg1	vesicles					192:199	Parasite-released extracellular vesicles	160:199	Parasite-released extracellular vesicles (EVs)	160:205	Parasite-released extracellular vesicles (EVs) deliver signals to the host immune system that are critical to maintaining the long-term relationship between parasite and host.					
32898175	2	30	theme	gravity	488:494	arg1	method					501:506	a recently described gravity flow method	467:506	a recently described gravity flow method that protects their structural integrity	467:547	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	4	31	contain	contained	738:746	arg1	121					725:727	121	725:727	121	725:727	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	4	31	contain	contained	738:746	arg1	proteins					715:722	618 proteins	711:722	618 proteins	711:722	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	4	31	contain	contained	738:746	arg2	sites					780:784	putative N-linked glycosylation sites	748:784	putative N-linked glycosylation sites	748:784	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	0	32	theme	gravity	92:98	arg1	method					105:110	a gravity flow method	90:110	a gravity flow method	90:110	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.					
32898175	2	33	theme	structural	528:537	arg1	integrity					539:547	their structural integrity	522:547	their structural integrity	522:547	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	4	34	gly	glycosylation	766:778	arg2	sites					780:784	putative N-linked glycosylation sites	748:784	putative N-linked glycosylation sites	748:784	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	6	35	theme	enhanced	1239:1246	arg1	expression					1248:1257	enhanced expression	1239:1257	enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1)	1239:1319	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	7	36	theme	non-specific	1570:1581	arg1	stimulation					1591:1601	both specific and non-specific antigen stimulation	1552:1601	both specific and non-specific antigen stimulation	1552:1601	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	4	37	theme	putative	815:822	arg1	sites					847:851	putative O-linked glycosylation sites	815:851	putative O-linked glycosylation sites	815:851	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	0	38	theme	dendritic	121:129	arg1	cell					131:134	dendritic cell phenotype and activity	121:157	cell	131:134	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.					
32898175	5	39	theme	high	1047:1050	arg1	affinity					1052:1059	a high affinity	1045:1059	a high affinity for complex-type N-glycans	1045:1086	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	8	40	dep	alum	1724:1727	arg1	presence					1712:1719	presence	1712:1719	presence	1712:1719	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	8	40	dep	alum	1724:1727	arg1	the					1708:1710	the	1708:1710	the	1708:1710	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	4	41	theme	glycosylation	766:778	arg1	sites					780:784	putative N-linked glycosylation sites	748:784	putative N-linked glycosylation sites	748:784	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	0	42	theme	Fasciola	0:7	arg1	hepatica					9:16	Fasciola hepatica	0:16	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method	0:110	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.					
32898175	2	43	theme	Fasciola	429:436	arg1	hepatica					438:445	the helminth parasite Fasciola hepatica	407:445	the helminth parasite Fasciola hepatica	407:445	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	2	44	theme	helminth	411:418	arg1	hepatica					438:445	the helminth parasite Fasciola hepatica	407:445	the helminth parasite Fasciola hepatica	407:445	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	5	45	theme	surface-exposed	877:891	arg1	glycans					893:899	surface-exposed glycans	877:899	surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins	877:1012	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	5	46	theme	other	1026:1030	arg1	glycans					1032:1038	other glycans	1026:1038	other glycans with a high affinity for complex-type N-glycans	1026:1086	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	6	47	theme	dendritic	1123:1131	arg1	cells					1133:1137	bone-marrow derived dendritic cells	1103:1137	bone-marrow derived dendritic cells isolated FhEV	1103:1151	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	5	48	with	glycans	893:899	arg1	affinity					1052:1059	a high affinity	1045:1059	a high affinity for complex-type N-glycans	1045:1086	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	5	48	with	glycans	893:899	arg1	affinity					913:920	a high affinity	906:920	a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins	906:1012	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	4	49	gly	glycosylation	833:845	arg2	sites					847:851	putative O-linked glycosylation sites	815:851	putative O-linked glycosylation sites	815:851	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	6	50	theme	bone-marrow	1103:1113	arg1	cells					1133:1137	bone-marrow derived dendritic cells	1103:1137	bone-marrow derived dendritic cells isolated FhEV	1103:1151	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	3	51	theme	glycan	657:662	arg1	microarrays					664:674	glycan microarrays	657:674	glycan microarrays	657:674	The FhEVs molecular cargo was defined using proteomic analysis and their surface topology characterised by glycan microarrays.					
32898175	2	52	theme	total	358:362	arg1	FhEVs					369:373	FhEVs	369:373	FhEVs	369:373	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	2	52	theme	total	358:362	arg1	EVs					364:366	total EVs	358:366	total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica	358:445	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	0	53	theme	excretory-secretory	55:73	arg1	products					75:82	excretory-secretory products	55:82	excretory-secretory products using a gravity flow method	55:110	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.					
32898175	10	54	theme	vaccines	2070:2077	arg1	discovery					2041:2049	the discovery	2037:2049	the discovery of future parasite vaccines and immunotherapeutic biologicals	2037:2111	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	4	55	theme	glycosylation	833:845	arg1	sites					847:851	putative O-linked glycosylation sites	815:851	putative O-linked glycosylation sites	815:851	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	8	56	theme	significant	1666:1676	arg1	responses					1685:1693	significant immune responses	1666:1693	significant immune responses	1666:1693	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	10	57	theme	future	2054:2059	arg1	vaccines					2070:2077	future parasite vaccines	2054:2077	future parasite vaccines	2054:2077	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	4	58	link	O-linked	824:831	arg1	sites					847:851	putative O-linked glycosylation sites	815:851	putative O-linked glycosylation sites	815:851	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	7	59	theme	lymph	1494:1498	arg1	nodes					1500:1504	skin draining lymph nodes	1480:1504	skin draining lymph nodes	1480:1504	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	4	60	link	N-linked	757:764	arg1	sites					780:784	putative N-linked glycosylation sites	748:784	putative N-linked glycosylation sites	748:784	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	10	61	theme	immunotherapeutic	2083:2099	arg1	biologicals					2101:2111	immunotherapeutic biologicals	2083:2111	immunotherapeutic biologicals	2083:2111	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	8	62	theme	FhEV	1646:1649	arg1	suspension					1632:1641	a suspension	1630:1641	a suspension of FhEV	1630:1649	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	7	63	theme	skin	1480:1483	arg1	nodes					1500:1504	skin draining lymph nodes	1480:1504	skin draining lymph nodes	1480:1504	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	7	64	theme	IL-2	1460:1463	arg1	secretion					1465:1473	IL-2 secretion	1460:1473	IL-2 secretion from skin draining lymph nodes and spleen cells	1460:1521	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	0	65	attach	isolated	41:48	arg2	Vesicles					32:39	Fasciola hepatica Extracellular Vesicles	0:39	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method	0:110	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.					
32898175	0	65	attach	isolated	41:48	arg1	products					75:82	excretory-secretory products	55:82	excretory-secretory products using a gravity flow method	55:110	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.					
32898175	6	66	theme	TNF	1234:1236	arg1	levels					1224:1229	low levels	1220:1229	low levels of TNF	1220:1236	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	9	67	theme	IL-2	1914:1917	arg1	secretion					1919:1927	IL-2 secretion	1914:1927	IL-2 secretion from T-cells	1914:1940	Thus, we have demonstrated that FhEVs induce a unique phentotype in DC capable of suppressing IL-2 secretion from T-cells.					
32898175	5	68	theme	mannose-binding	926:940	arg1	lectins					942:948	mannose-binding lectins	926:948	mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins	926:1012	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	6	69	theme	levels	1224:1229	arg1	expression					1248:1257	enhanced expression	1239:1257	enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1)	1239:1319	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	69	theme	levels	1224:1229	arg1	elevation					1325:1333	elevation	1325:1333	elevation of intracellular markers (SOCS1 and SOCS3)	1325:1376	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	69	theme	levels	1224:1229	arg1	secretion					1207:1215	the secretion	1203:1215	the secretion of low levels of TNF	1203:1236	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	3	70	theme	molecular	560:568	arg1	cargo					570:574	The FhEVs molecular cargo	550:574	The FhEVs molecular cargo	550:574	The FhEVs molecular cargo was defined using proteomic analysis and their surface topology characterised by glycan microarrays.					
32898175	7	71	theme	OT-II	1427:1431	arg1	mice					1433:1436	OT-II mice	1427:1436	OT-II mice	1427:1436	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	1	72	theme	host	230:233	arg1	system					242:247	the host immune system	226:247	the host immune system	226:247	Parasite-released extracellular vesicles (EVs) deliver signals to the host immune system that are critical to maintaining the long-term relationship between parasite and host.					
32898175	7	73	from	nodes	1500:1504	arg1	secretion					1465:1473	IL-2 secretion	1460:1473	IL-2 secretion from skin draining lymph nodes and spleen cells	1460:1521	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	5	74	gly	glycoproteins	1000:1012	arg1	glycoproteins					1000:1012	oligo mannose-rich glycoproteins	981:1012	oligo mannose-rich glycoproteins	981:1012	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	5	75	with	glycans	1032:1038	arg1	affinity					1052:1059	a high affinity	1045:1059	a high affinity for complex-type N-glycans	1045:1086	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	5	75	with	glycans	1032:1038	arg1	affinity					913:920	a high affinity	906:920	a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins	906:1012	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	6	76	theme	intracellular	1338:1350	arg1	SOCS3					1371:1375	SOCS3	1371:1375	SOCS3	1371:1375	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	76	theme	intracellular	1338:1350	arg1	SOCS1					1361:1365	SOCS1	1361:1365	SOCS1	1361:1365	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	76	theme	intracellular	1338:1350	arg1	markers					1352:1358	intracellular markers	1338:1358	intracellular markers (SOCS1 and SOCS3)	1338:1376	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	8	77	theme	Th1/Th2	1752:1758	arg1	response					1767:1774	a mixed Th1/Th2 immune response	1744:1774	a mixed Th1/Th2 immune response	1744:1774	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	5	78	theme	oligo	981:985	arg1	glycoproteins					1000:1012	oligo mannose-rich glycoproteins	981:1012	oligo mannose-rich glycoproteins	981:1012	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	9	79	from	T-cells	1934:1940	arg1	secretion					1919:1927	IL-2 secretion	1914:1927	IL-2 secretion from T-cells	1914:1940	Thus, we have demonstrated that FhEVs induce a unique phentotype in DC capable of suppressing IL-2 secretion from T-cells.					
32898175	10	80	theme	growing	1966:1972	arg1	database					1991:1998	the growing immuno-proteomic database	1962:1998	the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals	1962:2111	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	10	80	theme	growing	1966:1972	arg1	source					2026:2031	an important source	2013:2031	an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals	2013:2111	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	8	81	with	mice	1620:1623	arg1	suspension					1632:1641	a suspension	1630:1641	a suspension of FhEV	1630:1649	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	5	82	theme	glycoproteins	1000:1012	arg1	predominance					965:976	the predominance	961:976	the predominance of oligo mannose-rich glycoproteins	961:1012	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	6	83	theme	surface	1267:1273	arg1	markers					1275:1281	cell surface markers	1262:1281	cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1)	1262:1319	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	83	theme	surface	1267:1273	arg1	CD40					1296:1299	CD40	1296:1299	CD40	1296:1299	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	83	theme	surface	1267:1273	arg1	OX40L					1302:1306	OX40L	1302:1306	OX40L	1302:1306	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	83	theme	surface	1267:1273	arg1	CD80					1284:1287	CD80	1284:1287	CD80	1284:1287	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	83	theme	surface	1267:1273	arg1	CD86					1290:1293	CD86	1290:1293	CD86	1290:1293	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	83	theme	surface	1267:1273	arg1	SIGNR1					1313:1318	SIGNR1	1313:1318	SIGNR1	1313:1318	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	8	84	theme	high	1781:1784	arg1	titres					1812:1817	high antigen specific antibody titres	1781:1817	high antigen specific antibody titres	1781:1817	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	1	85	theme	Parasite-released	160:176	arg1	EVs					202:204	EVs	202:204	EVs	202:204	Parasite-released extracellular vesicles (EVs) deliver signals to the host immune system that are critical to maintaining the long-term relationship between parasite and host.					
32898175	1	85	theme	Parasite-released	160:176	arg1	vesicles					192:199	Parasite-released extracellular vesicles	160:199	Parasite-released extracellular vesicles (EVs)	160:205	Parasite-released extracellular vesicles (EVs) deliver signals to the host immune system that are critical to maintaining the long-term relationship between parasite and host.					
32898175	2	86	theme	flow	496:499	arg1	method					501:506	a recently described gravity flow method	467:506	a recently described gravity flow method that protects their structural integrity	467:547	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	0	87	theme	flow	100:103	arg1	method					105:110	a gravity flow method	90:110	a gravity flow method	90:110	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.					
32898175	8	88	theme	specific	1794:1801	arg1	titres					1812:1817	high antigen specific antibody titres	1781:1817	high antigen specific antibody titres	1781:1817	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	0	89	theme	Extracellular	18:30	arg1	Vesicles					32:39	Fasciola hepatica Extracellular Vesicles	0:39	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method	0:110	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.					
32898175	7	90	theme	antigen	1583:1589	arg1	stimulation					1591:1601	both specific and non-specific antigen stimulation	1552:1601	both specific and non-specific antigen stimulation	1552:1601	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	2	91	theme	described	478:486	arg1	method					501:506	a recently described gravity flow method	467:506	a recently described gravity flow method that protects their structural integrity	467:547	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	0	92	dep	cell	131:134	arg1	phenotype					136:144	phenotype	136:144	phenotype	136:144	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.					
32898175	4	93	theme	132	792:794	arg1	proteins					796:803	132 proteins	792:803	132 proteins	792:803	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	5	94	theme	Glycan	854:859	arg1	arrays					861:866	Glycan arrays	854:866	Glycan arrays	854:866	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	2	95	theme	hepatica	438:445	arg1	adults					397:402	adults	397:402	adults of the helminth parasite Fasciola hepatica	397:445	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	4	96	theme	N-linked	757:764	arg1	sites					780:784	putative N-linked glycosylation sites	748:784	putative N-linked glycosylation sites	748:784	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	2	97	theme	parasite	420:427	arg1	hepatica					438:445	the helminth parasite Fasciola hepatica	407:445	the helminth parasite Fasciola hepatica	407:445	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	7	98	theme	specific	1557:1564	arg1	stimulation					1591:1601	both specific and non-specific antigen stimulation	1552:1601	both specific and non-specific antigen stimulation	1552:1601	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	6	99	theme	derived	1115:1121	arg1	cells					1133:1137	bone-marrow derived dendritic cells	1103:1137	bone-marrow derived dendritic cells isolated FhEV	1103:1151	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	100	dep	markers	1352:1358	arg1	SOCS3					1371:1375	SOCS3	1371:1375	SOCS3	1371:1375	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	100	dep	markers	1352:1358	arg1	SOCS1					1361:1365	SOCS1	1361:1365	SOCS1	1361:1365	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	6	100	dep	markers	1352:1358	arg1	markers					1352:1358	intracellular markers	1338:1358	intracellular markers (SOCS1 and SOCS3)	1338:1376	When added to bone-marrow derived dendritic cells isolated FhEV induced a novel phenotype that was categorised by the secretion of low levels of TNF, enhanced expression of cell surface markers (CD80, CD86, CD40, OX40L, and SIGNR1) and elevation of intracellular markers (SOCS1 and SOCS3).					
32898175	8	101	theme	mice	1620:1623	arg1	Immunisation					1604:1615	Immunisation	1604:1615	Immunisation of mice with a suspension of FhEV	1604:1649	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	8	102	theme	immune	1678:1683	arg1	responses					1685:1693	significant immune responses	1666:1693	significant immune responses	1666:1693	Immunisation of mice with a suspension of FhEV did not elicit significant immune responses; however, in the presence of alum, FhEVs induced a mixed Th1/Th2 immune response with high antigen specific antibody titres.					
32898175	10	103	theme	parasite	2061:2068	arg1	vaccines					2070:2077	future parasite vaccines	2054:2077	future parasite vaccines	2054:2077	Our studies add to the growing immuno-proteomic database that will be an important source for the discovery of future parasite vaccines and immunotherapeutic biologicals.					
32898175	4	104	theme	O-linked	824:831	arg1	sites					847:851	putative O-linked glycosylation sites	815:851	putative O-linked glycosylation sites	815:851	The proteomic analysis identified 618 proteins, 121 of which contained putative N-linked glycosylation sites while 132 proteins contained putative O-linked glycosylation sites.					
32898175	2	105	theme	present	343:349	arg1	study					351:355	the present study	339:355	the present study	339:355	In the present study, total EVs (FhEVs) released in vitro by adults of the helminth parasite Fasciola hepatica were isolated using a recently described gravity flow method that protects their structural integrity.					
32898175	7	106	from	cells	1517:1521	arg1	secretion					1465:1473	IL-2 secretion	1460:1473	IL-2 secretion from skin draining lymph nodes and spleen cells	1460:1521	When FhEV-stimulated BMDCs were introduced into OT-II mice by adoptive transfer, IL-2 secretion from skin draining lymph nodes and spleen cells was inhibited in response to both specific and non-specific antigen stimulation.					
32898175	1	107	theme	immune	235:240	arg1	system					242:247	the host immune system	226:247	the host immune system	226:247	Parasite-released extracellular vesicles (EVs) deliver signals to the host immune system that are critical to maintaining the long-term relationship between parasite and host.					
32898175	5	108	theme	complex-type	1065:1076	arg1	N-glycans					1078:1086	complex-type N-glycans	1065:1086	complex-type N-glycans	1065:1086	Glycan arrays revealed surface-exposed glycans with a high affinity for mannose-binding lectins indicating the predominance of oligo mannose-rich glycoproteins, as well as other glycans with a high affinity for complex-type N-glycans.					
32898175	9	109	theme	unique	1867:1872	arg1	phentotype					1874:1883	a unique phentotype	1865:1883	a unique phentotype	1865:1883	Thus, we have demonstrated that FhEVs induce a unique phentotype in DC capable of suppressing IL-2 secretion from T-cells.					
32898175	0	110	theme	hepatica	9:16	arg1	Vesicles					32:39	Fasciola hepatica Extracellular Vesicles	0:39	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method	0:110	Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.					
32811831	4	0	theme	strategy	536:543	arg1	combination					512:522	The optimal combination	500:522	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction	500:601	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction has enabled us to synthesize up to a 128-mer glycan.					
32811831	4	1	theme	128-mer	640:646	arg1	glycan					648:653	a 128-mer glycan	638:653	a 128-mer glycan	638:653	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction has enabled us to synthesize up to a 128-mer glycan.					
32811831	4	2	theme	assembly	527:534	arg1	strategy					536:543	assembly strategy	527:543	assembly strategy	527:543	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction has enabled us to synthesize up to a 128-mer glycan.					
32811831	0	3	theme	Bacteroides	75:85	arg1	vulgatus					87:94	Bacteroides vulgatus	75:94	Bacteroides vulgatus	75:94	Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus.					
32811831	3	4	theme	common	464:469	arg1	vulgatus					452:459	Bacteroides vulgatus	440:459	Bacteroides vulgatus	440:459	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	3	4	theme	common	464:469	arg1	component					471:479	a common component	462:479	a common component of gut microbiota	462:497	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	3	5	theme	microbiota	488:497	arg1	vulgatus					452:459	Bacteroides vulgatus	440:459	Bacteroides vulgatus	440:459	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	3	5	theme	microbiota	488:497	arg1	component					471:479	a common component	462:479	a common component of gut microbiota	462:497	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	4	6	theme	protecting	546:555	arg1	arrangement					563:573	protecting group arrangement	546:573	protecting group arrangement	546:573	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction has enabled us to synthesize up to a 128-mer glycan.					
32811831	4	7	theme	glycosylation	580:592	arg1	reaction					594:601	glycosylation reaction	580:601	glycosylation reaction	580:601	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction has enabled us to synthesize up to a 128-mer glycan.					
32811831	5	8	theme	human	812:816	arg1	receptor					829:836	a receptor	827:836	a receptor associated with the gut lymphoid tissue	827:876	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	5	8	theme	human	812:816	arg1	DC-SIGN					818:824	human DC-SIGN	812:824	human DC-SIGN	812:824	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	0	9	theme	vulgatus	87:94	arg1	O-antigen					62:70	the O-antigen	58:70	the O-antigen of Bacteroides vulgatus	58:94	Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus.					
32811831	3	10	theme	gut	484:486	arg1	microbiota					488:497	gut microbiota	484:497	gut microbiota	484:497	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	1	11	theme	critical	158:165	arg1	targets					167:173	critical targets	158:173	critical targets of biomedical developments	158:200	Glycans are involved in various life processes and represent critical targets of biomedical developments.					
32811831	3	12	theme	1 → 3	365:369	arg1	unit					395:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	3	13	theme	repeating	385:393	arg1	unit					395:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	0	14	theme	Chemical	0:7	arg1	synthesis					9:17	Chemical synthesis	0:17	Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus.	0:95	Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus.					
32811831	4	15	theme	optimal	504:510	arg1	combination					512:522	The optimal combination	500:522	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction	500:601	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction has enabled us to synthesize up to a 128-mer glycan.					
32811831	3	16	theme	-α-Rha-	357:363	arg1	unit					395:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	1	17	theme	biomedical	178:187	arg1	developments					189:200	biomedical developments	178:200	biomedical developments	178:200	Glycans are involved in various life processes and represent critical targets of biomedical developments.					
32811831	1	18	theme	developments	189:200	arg1	targets					167:173	critical targets	158:173	critical targets of biomedical developments	158:200	Glycans are involved in various life processes and represent critical targets of biomedical developments.					
32811831	3	19	theme	1 → 	379:382	arg1	unit					395:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	2	20	with	accessibility	221:233	arg1	structures					264:273	precise structures	256:273	precise structures	256:273	Nevertheless, the accessibility to long glycans with precise structures remains challenging.					
32811831	5	21	theme	synthetic	660:668	arg1	glycans					670:676	The synthetic glycans	656:676	The synthetic glycans	656:676	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	0	22	dep	128-mer	38:44	arg1	up					30:31	up	30:31	up	30:31	Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus.					
32811831	5	23	theme	potent	783:788	arg1	activity					798:805	their potent binding activity	777:805	their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue,	777:877	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	5	24	dep	NMR	719:721	arg1	approaches					730:739	approaches	730:739	approaches	730:739	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	0	25	theme	glycans	22:28	arg1	synthesis					9:17	Chemical synthesis	0:17	Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus.	0:95	Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus.					
32811831	5	26	theme	binding	790:796	arg1	activity					798:805	their potent binding activity	777:805	their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue,	777:877	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	3	27	theme	-β-Man-	371:377	arg1	unit					395:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	3	28	theme	[→4	353:355	arg1	unit					395:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	[→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit	353:398	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	0	29	theme	relevant	46:53	arg1	128-mer					38:44	a 128-mer	36:44	a 128-mer relevant to the O-antigen of Bacteroides vulgatus	36:94	Chemical synthesis of glycans up to a 128-mer relevant to the O-antigen of Bacteroides vulgatus.					
32811831	5	30	theme	3D	746:747	arg1	structures					749:758	the 3D structures	742:758	the 3D structures	742:758	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	3	31	theme	vulgatus	452:459	arg1	O-antigen					427:435	the O-antigen	423:435	the O-antigen of Bacteroides vulgatus, a common component of gut microbiota	423:497	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	5	32	with	activity	798:805	arg1	receptor					829:836	a receptor	827:836	a receptor associated with the gut lymphoid tissue	827:876	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	5	32	with	activity	798:805	arg1	DC-SIGN					818:824	human DC-SIGN	812:824	human DC-SIGN	812:824	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	2	33	theme	precise	256:262	arg1	structures					264:273	precise structures	256:273	precise structures	256:273	Nevertheless, the accessibility to long glycans with precise structures remains challenging.					
32811831	5	34	theme	gut	858:860	arg1	tissue					871:876	the gut lymphoid tissue	854:876	the gut lymphoid tissue	854:876	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	4	35	theme	arrangement	563:573	arg1	combination					512:522	The optimal combination	500:522	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction	500:601	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction has enabled us to synthesize up to a 128-mer glycan.					
32811831	5	36	theme	lymphoid	862:869	arg1	tissue					871:876	the gut lymphoid tissue	854:876	the gut lymphoid tissue	854:876	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	4	37	theme	group	557:561	arg1	arrangement					563:573	protecting group arrangement	546:573	protecting group arrangement	546:573	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction has enabled us to synthesize up to a 128-mer glycan.					
32811831	5	38	theme	advanced	710:717	arg1	NMR					719:721	advanced NMR	710:721	advanced NMR	710:721	The synthetic glycans are accurately characterized by advanced NMR and MS approaches, the 3D structures are defined, and their potent binding activity with human DC-SIGN, a receptor associated with the gut lymphoid tissue, is disclosed.					
32811831	4	39	theme	reaction	594:601	arg1	combination					512:522	The optimal combination	500:522	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction	500:601	The optimal combination of assembly strategy, protecting group arrangement, and glycosylation reaction has enabled us to synthesize up to a 128-mer glycan.					
32811831	1	40	theme	various	121:127	arg1	processes					134:142	various life processes	121:142	various life processes	121:142	Glycans are involved in various life processes and represent critical targets of biomedical developments.					
32811831	3	41	theme	glycans	331:337	arg1	synthesis					318:326	the synthesis	314:326	the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota	314:497	Here we report on the synthesis of glycans consisting of [→4)-α-Rha-(1 → 3)-β-Man-(1 → ] repeating unit, which are relevant to the O-antigen of Bacteroides vulgatus, a common component of gut microbiota.					
32811831	2	42	theme	long	238:241	arg1	glycans					243:249	long glycans	238:249	long glycans	238:249	Nevertheless, the accessibility to long glycans with precise structures remains challenging.					
32811831	1	43	theme	life	129:132	arg1	processes					134:142	various life processes	121:142	various life processes	121:142	Glycans are involved in various life processes and represent critical targets of biomedical developments.					
32185836	7	0	theme	InlB	1074:1077	arg1	retention					1087:1095	the InlB surface retention	1070:1095	the InlB surface retention	1070:1095	We evaluated the InlB surface retention in mutants lacking each of these two genes, and found that only galactosylated WTA is required for InlB surface presentation and function, cellular invasiveness and phage adsorption, while galactosylated LTA plays no role thereof.					
32185836	6	1	dep	genes	972:976	arg1	genes					972:976	two genes	968:976	two genes gtlB and gttB	968:990	Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively.					
32185836	6	1	dep	genes	972:976	arg1	gttB					987:990	gttB	987:990	gttB	987:990	Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively.					
32185836	6	1	dep	genes	972:976	arg1	gtlB					978:981	gtlB	978:981	gtlB	978:981	Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively.					
32185836	7	2	theme	surface	1079:1085	arg1	retention					1087:1095	the InlB surface retention	1070:1095	the InlB surface retention	1070:1095	We evaluated the InlB surface retention in mutants lacking each of these two genes, and found that only galactosylated WTA is required for InlB surface presentation and function, cellular invasiveness and phage adsorption, while galactosylated LTA plays no role thereof.					
32185836	6	3	theme	WTA	1039:1041	arg1	galactosylation					1012:1026	galactosylation	1012:1026	galactosylation of LTA and WTA respectively	1012:1054	Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively.					
32185836	8	4	theme	virulence	1514:1522	arg1	factor					1524:1529	an important virulence factor	1501:1529	an important virulence factor	1501:1529	Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.					
32185836	4	5	theme	serovar	733:739	arg1	determination					741:753	serovar determination	733:753	serovar determination	733:753	In contrast, the membrane-anchored lipoteichoic acids (LTAs) do not show significant variation and do not contribute to serovar determination.					
32185836	6	6	theme	4b	957:958	arg1	strain					960:965	a highly virulent serovar 4b strain	931:965	a highly virulent serovar 4b strain	931:965	Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively.					
32185836	7	7	theme	surface	1201:1207	arg1	presentation					1209:1220	InlB surface presentation	1196:1220	InlB surface presentation	1196:1220	We evaluated the InlB surface retention in mutants lacking each of these two genes, and found that only galactosylated WTA is required for InlB surface presentation and function, cellular invasiveness and phage adsorption, while galactosylated LTA plays no role thereof.					
32185836	5	8	theme	surface-associated	792:809	arg1	InlB					811:814	surface-associated InlB	792:814	surface-associated InlB	792:814	It was previously demonstrated that surface-associated InlB non-covalently adheres to both WTA and LTA, mediating its retention on the cell wall.					
32185836	3	9	theme	physiological	470:482	arg1	functions					484:492	many physiological functions	465:492	many physiological functions	465:492	WTAs have many physiological functions; they can serve as receptors for bacteriophages, and provide a substrate for binding of surface proteins such as InlB.					
32185836	2	10	gly	glycosylated	377:388	arg1	WTAs					337:340	WTAs	337:340	WTAs	337:340	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	2	10	gly	glycosylated	377:388	arg1	acids					330:334	Structurally diverse wall teichoic acids	295:334	Structurally diverse wall teichoic acids (WTAs)	295:341	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	5	11	from	retention	874:882	arg1	wall					896:899	the cell wall	887:899	the cell wall	887:899	It was previously demonstrated that surface-associated InlB non-covalently adheres to both WTA and LTA, mediating its retention on the cell wall.					
32185836	7	12	theme	galactosylated	1286:1299	arg1	LTA					1301:1303	galactosylated LTA	1286:1303	galactosylated LTA	1286:1303	We evaluated the InlB surface retention in mutants lacking each of these two genes, and found that only galactosylated WTA is required for InlB surface presentation and function, cellular invasiveness and phage adsorption, while galactosylated LTA plays no role thereof.					
32185836	4	13	theme	membrane-anchored	630:646	arg1	LTAs					668:671	LTAs	668:671	LTAs	668:671	In contrast, the membrane-anchored lipoteichoic acids (LTAs) do not show significant variation and do not contribute to serovar determination.					
32185836	4	13	theme	membrane-anchored	630:646	arg1	acids					661:665	the membrane-anchored lipoteichoic acids	626:665	the membrane-anchored lipoteichoic acids (LTAs)	626:672	In contrast, the membrane-anchored lipoteichoic acids (LTAs) do not show significant variation and do not contribute to serovar determination.					
32185836	6	14	theme	serovar	949:955	arg1	strain					960:965	a highly virulent serovar 4b strain	931:965	a highly virulent serovar 4b strain	931:965	Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively.					
32185836	2	15	theme	various	428:434	arg1	serovars					445:452	the various Listeria serovars	424:452	the various Listeria serovars	424:452	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	1	16	theme	Gram-positive	156:168	arg1	monocytogenes					137:149	Listeria monocytogenes	128:149	Listeria monocytogenes	128:149	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	1	16	theme	Gram-positive	156:168	arg1	pathogen					185:192	a Gram-positive, intracellular pathogen	154:192	pathogen	185:192	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	0	17	theme	wall	15:18	arg1	acid					29:32	Galactosylated wall teichoic acid	0:32	Galactosylated wall teichoic acid	0:32	Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes.					
32185836	6	18	theme	virulent	940:947	arg1	strain					960:965	a highly virulent serovar 4b strain	931:965	a highly virulent serovar 4b strain	931:965	Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively.					
32185836	2	19	theme	teichoic	321:328	arg1	WTAs					337:340	WTAs	337:340	WTAs	337:340	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	2	19	theme	teichoic	321:328	arg1	acids					330:334	Structurally diverse wall teichoic acids	295:334	Structurally diverse wall teichoic acids (WTAs)	295:341	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	7	20	theme	phage	1262:1266	arg1	adsorption					1268:1277	phage adsorption	1262:1277	phage adsorption	1262:1277	We evaluated the InlB surface retention in mutants lacking each of these two genes, and found that only galactosylated WTA is required for InlB surface presentation and function, cellular invasiveness and phage adsorption, while galactosylated LTA plays no role thereof.					
32185836	0	21	theme	Galactosylated	0:13	arg1	acid					29:32	Galactosylated wall teichoic acid	0:32	Galactosylated wall teichoic acid	0:32	Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes.					
32185836	1	22	theme	certain	275:281	arg1	cells					288:292	certain host cells	275:292	certain host cells	275:292	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	4	23	theme	significant	686:696	arg1	variation					698:706	significant variation	686:706	significant variation	686:706	In contrast, the membrane-anchored lipoteichoic acids (LTAs) do not show significant variation and do not contribute to serovar determination.					
32185836	2	24	theme	wall	316:319	arg1	WTAs					337:340	WTAs	337:340	WTAs	337:340	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	2	24	theme	wall	316:319	arg1	acids					330:334	Structurally diverse wall teichoic acids	295:334	Structurally diverse wall teichoic acids (WTAs)	295:341	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	1	25	theme	intracellular	171:183	arg1	monocytogenes					137:149	Listeria monocytogenes	128:149	Listeria monocytogenes	128:149	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	1	25	theme	intracellular	171:183	arg1	pathogen					185:192	a Gram-positive, intracellular pathogen	154:192	pathogen	185:192	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	1	26	theme	host	283:286	arg1	cells					288:292	certain host cells	275:292	certain host cells	275:292	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	0	27	theme	4b	101:102	arg1	monocytogenes					113:125	serovar 4b Listeria monocytogenes	93:125	serovar 4b Listeria monocytogenes	93:125	Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes.					
32185836	6	28	from	responsible	996:1006	arg1	strain					960:965	a highly virulent serovar 4b strain	931:965	a highly virulent serovar 4b strain	931:965	Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively.					
32185836	2	29	theme	diverse	308:314	arg1	WTAs					337:340	WTAs	337:340	WTAs	337:340	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	2	29	theme	diverse	308:314	arg1	acids					330:334	Structurally diverse wall teichoic acids	295:334	Structurally diverse wall teichoic acids (WTAs)	295:341	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	0	30	theme	teichoic	20:27	arg1	acid					29:32	Galactosylated wall teichoic acid	0:32	Galactosylated wall teichoic acid	0:32	Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes.					
32185836	0	31	theme	serovar	93:99	arg1	monocytogenes					113:125	serovar 4b Listeria monocytogenes	93:125	serovar 4b Listeria monocytogenes	93:125	Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes.					
32185836	2	32	theme	antigenic	405:413	arg1	basis					415:419	the antigenic basis	401:419	the antigenic basis of the various Listeria serovars	401:452	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	8	33	theme	factor	1524:1529	arg1	function					1489:1496	the function	1485:1496	the function of an important virulence factor	1485:1529	Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.					
32185836	0	34	theme	monocytogenes	113:125	arg1	surface					82:88	the surface	78:88	the surface of serovar 4b Listeria monocytogenes	78:125	Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes.					
32185836	4	35	theme	lipoteichoic	648:659	arg1	LTAs					668:671	LTAs	668:671	LTAs	668:671	In contrast, the membrane-anchored lipoteichoic acids (LTAs) do not show significant variation and do not contribute to serovar determination.					
32185836	4	35	theme	lipoteichoic	648:659	arg1	acids					661:665	the membrane-anchored lipoteichoic acids	626:665	the membrane-anchored lipoteichoic acids (LTAs)	626:672	In contrast, the membrane-anchored lipoteichoic acids (LTAs) do not show significant variation and do not contribute to serovar determination.					
32185836	0	36	theme	Listeria	104:111	arg1	monocytogenes					113:125	serovar 4b Listeria monocytogenes	93:125	serovar 4b Listeria monocytogenes	93:125	Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes.					
32185836	3	37	contain	have	460:463	arg1	WTAs					455:458	WTAs	455:458	WTAs	455:458	WTAs have many physiological functions; they can serve as receptors for bacteriophages, and provide a substrate for binding of surface proteins such as InlB.					
32185836	3	37	contain	have	460:463	arg2	functions					484:492	many physiological functions	465:492	many physiological functions	465:492	WTAs have many physiological functions; they can serve as receptors for bacteriophages, and provide a substrate for binding of surface proteins such as InlB.					
32185836	3	38	theme	surface	582:588	arg1	proteins					590:597	surface proteins	582:597	surface proteins such as InlB	582:610	WTAs have many physiological functions; they can serve as receptors for bacteriophages, and provide a substrate for binding of surface proteins such as InlB.					
32185836	3	38	theme	surface	582:588	arg1	InlB					607:610	InlB	607:610	InlB	607:610	WTAs have many physiological functions; they can serve as receptors for bacteriophages, and provide a substrate for binding of surface proteins such as InlB.					
32185836	1	39	theme	surface-associated	208:225	arg1	InlB					244:247	the surface-associated virulence factor InlB	204:247	the surface-associated virulence factor InlB	204:247	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	0	40	theme	lipoteichoic	43:54	arg1	acid					56:59	lipoteichoic acid	43:59	lipoteichoic acid	43:59	Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes.					
32185836	8	41	theme	important	1504:1512	arg1	factor					1524:1529	an important virulence factor	1501:1529	an important virulence factor	1501:1529	Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.					
32185836	5	42	theme	cell	891:894	arg1	wall					896:899	the cell wall	887:899	the cell wall	887:899	It was previously demonstrated that surface-associated InlB non-covalently adheres to both WTA and LTA, mediating its retention on the cell wall.					
32185836	2	43	theme	serovars	445:452	arg1	basis					415:419	the antigenic basis	401:419	the antigenic basis of the various Listeria serovars	401:452	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
32185836	6	44	from	strain	960:965	arg1	responsible					996:1006	responsible	996:1006	responsible	996:1006	Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively.					
32185836	8	45	theme	bacteriophage	1416:1428	arg1	susceptibility					1430:1443	bacteriophage susceptibility	1416:1443	bacteriophage susceptibility	1416:1443	Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.					
32185836	7	46	theme	InlB	1196:1199	arg1	presentation					1209:1220	InlB surface presentation	1196:1220	InlB surface presentation	1196:1220	We evaluated the InlB surface retention in mutants lacking each of these two genes, and found that only galactosylated WTA is required for InlB surface presentation and function, cellular invasiveness and phage adsorption, while galactosylated LTA plays no role thereof.					
32185836	8	47	theme	pathogen-defining	1367:1383	arg1	necessary					1449:1457	necessary	1449:1457	necessary	1449:1457	Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.					
32185836	8	47	theme	pathogen-defining	1367:1383	arg1	antigen					1393:1399	a simple pathogen-defining serovar antigen	1358:1399	a simple pathogen-defining serovar antigen	1358:1399	Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.					
32185836	3	48	theme	many	465:468	arg1	functions					484:492	many physiological functions	465:492	many physiological functions	465:492	WTAs have many physiological functions; they can serve as receptors for bacteriophages, and provide a substrate for binding of surface proteins such as InlB.					
32185836	1	49	theme	virulence	227:235	arg1	InlB					244:247	the surface-associated virulence factor InlB	204:247	the surface-associated virulence factor InlB	204:247	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	8	50	theme	simple	1360:1365	arg1	necessary					1449:1457	necessary	1449:1457	necessary	1449:1457	Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.					
32185836	8	50	theme	simple	1360:1365	arg1	antigen					1393:1399	a simple pathogen-defining serovar antigen	1358:1399	a simple pathogen-defining serovar antigen	1358:1399	Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.					
32185836	1	51	theme	Listeria	128:135	arg1	monocytogenes					137:149	Listeria monocytogenes	128:149	Listeria monocytogenes	128:149	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	1	51	theme	Listeria	128:135	arg1	pathogen					185:192	a Gram-positive, intracellular pathogen	154:192	pathogen	185:192	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	1	52	theme	factor	237:242	arg1	InlB					244:247	the surface-associated virulence factor InlB	204:247	the surface-associated virulence factor InlB	204:247	Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells.					
32185836	7	53	theme	galactosylated	1161:1174	arg1	WTA					1176:1178	only galactosylated WTA	1156:1178	only galactosylated WTA	1156:1178	We evaluated the InlB surface retention in mutants lacking each of these two genes, and found that only galactosylated WTA is required for InlB surface presentation and function, cellular invasiveness and phage adsorption, while galactosylated LTA plays no role thereof.					
32185836	7	54	theme	cellular	1236:1243	arg1	invasiveness					1245:1256	cellular invasiveness	1236:1256	cellular invasiveness	1236:1256	We evaluated the InlB surface retention in mutants lacking each of these two genes, and found that only galactosylated WTA is required for InlB surface presentation and function, cellular invasiveness and phage adsorption, while galactosylated LTA plays no role thereof.					
32185836	3	55	theme	proteins	590:597	arg1	binding					571:577	binding	571:577	binding of surface proteins such as InlB	571:610	WTAs have many physiological functions; they can serve as receptors for bacteriophages, and provide a substrate for binding of surface proteins such as InlB.					
32185836	6	56	theme	LTA	1031:1033	arg1	galactosylation					1012:1026	galactosylation	1012:1026	galactosylation of LTA and WTA respectively	1012:1054	Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively.					
32185836	8	57	theme	serovar	1385:1391	arg1	necessary					1449:1457	necessary	1449:1457	necessary	1449:1457	Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.					
32185836	8	57	theme	serovar	1385:1391	arg1	antigen					1393:1399	a simple pathogen-defining serovar antigen	1358:1399	a simple pathogen-defining serovar antigen	1358:1399	Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.					
32185836	2	58	theme	Listeria	436:443	arg1	serovars					445:452	the various Listeria serovars	424:452	the various Listeria serovars	424:452	Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars.					
34143599	9	0	gly	glycosylation	1591:1603	arg1	protein					1612:1618	protein structure	1612:1628	protein structure	1612:1628	This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.					
34143599	5	1	dep	engineering	792:802	arg1	proteins					842:849	-targeted proteins	832:849	-targeted proteins	832:849	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	8	2	from	detection	1357:1365	arg1	mice					1411:1414	mice	1411:1414	mice with breast cancer	1411:1433	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	0	3	theme	Protein-Specific	110:125	arg1	Glycosylation					127:139	Amplified Visualization Protein-Specific Glycosylation	86:139	Amplified Visualization Protein-Specific Glycosylation in Mice	86:147	Proximity-Induced Hybridization Chain Reaction-Based Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice.					
34143599	7	4	theme	DNA	1217:1219	arg1	signals					1221:1227	DNA signals	1217:1227	DNA signals	1217:1227	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	3	5	dep	photoacoustic	490:502	arg1	PA					505:506	PA	505:506	PA	505:506	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	5	6	theme	glycan	711:716	arg1	probe					718:722	A glycan probe	709:722	A glycan probe (DBCO-GP)	709:732	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	5	6	theme	glycan	711:716	arg1	DBCO-GP					725:731	DBCO-GP	725:731	DBCO-GP	725:731	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	1	7	theme	several	207:213	arg1	functions					246:254	several physiological and pathological functions	207:254	several physiological and pathological functions	207:254	Glycosylation is a key cellular mechanism that regulates several physiological and pathological functions.					
34143599	6	8	dep	in	1025:1026	arg1	situ					1028:1031	situ	1028:1031	situ	1028:1031	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	8	9	theme	dynamic	1466:1472	arg1	states					1474:1479	its dynamic states	1462:1479	its dynamic states	1462:1479	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	6	10	theme	in	1025:1026	arg1	release					1033:1039	in situ release	1025:1039	in situ release of the HCR initiator domain	1025:1067	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	5	11	theme	metabolic	766:774	arg1	MOE					805:807	MOE	805:807	MOE	805:807	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	5	11	theme	metabolic	766:774	arg1	engineering					792:802	metabolic oligosaccharide engineering	766:802	metabolic oligosaccharide engineering (MOE)	766:808	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	6	12	theme	domain	928:933	arg1	hybridization					893:905	Proximity-induced hybridization	875:905	Proximity-induced hybridization of the complementary domain between the two kinds of probes	875:965	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	5	13	attach	attached	738:745	arg2	DBCO-GP					725:731	DBCO-GP	725:731	DBCO-GP	725:731	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	5	13	attach	attached	738:745	arg2	probe					718:722	A glycan probe	709:722	A glycan probe (DBCO-GP)	709:732	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	5	13	attach	attached	738:745	arg3	recognition					862:872	aptamer recognition	854:872	aptamer recognition	854:872	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	5	13	attach	attached	738:745	arg1	glycans					750:756	glycans	750:756	glycans	750:756	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	9	14	theme	disease	1681:1687	arg1	processes					1689:1697	disease processes	1681:1697	disease processes	1681:1697	This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.					
34143599	5	15	theme	oligosaccharide	776:790	arg1	MOE					805:807	MOE	805:807	MOE	805:807	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	5	15	theme	oligosaccharide	776:790	arg1	engineering					792:802	metabolic oligosaccharide engineering	766:802	metabolic oligosaccharide engineering (MOE)	766:808	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	1	16	theme	physiological	215:227	arg1	functions					246:254	several physiological and pathological functions	207:254	several physiological and pathological functions	207:254	Glycosylation is a key cellular mechanism that regulates several physiological and pathological functions.					
34143599	8	17	theme	breast	1421:1426	arg1	cancer					1428:1433	breast cancer	1421:1433	breast cancer	1421:1433	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	2	18	theme	personalized	427:438	arg1	modalities					452:461	personalized theranostic modalities	427:461	personalized theranostic modalities	427:461	Therefore, identification and characterization of specific-protein glycosylation in vivo are highly desirable for studying glycosylation-related pathology and developing personalized theranostic modalities.					
34143599	5	19	theme	-targeted	832:840	arg1	proteins					842:849	-targeted proteins	832:849	-targeted proteins	832:849	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	9	20	theme	glycosylation	1591:1603	arg1	effects					1580:1586	the effects	1576:1586	the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes	1576:1697	This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.					
34143599	6	21	theme	complementary	914:926	arg1	domain					928:933	the complementary domain	910:933	the complementary domain	910:933	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	7	22	dep	sequences	1123:1131	arg1	sequences					1123:1131	complementary sequences	1109:1131	complementary sequences (Au-H1 and Au-H2)	1109:1149	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	7	22	dep	sequences	1123:1131	arg1	Au-H2					1144:1148	Au-H2	1144:1148	Au-H2	1144:1148	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	7	22	dep	sequences	1123:1131	arg1	Au-H1					1134:1138	Au-H1	1134:1138	Au-H1	1134:1138	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	3	23	theme	hybridization	550:562	arg1	HCR					580:582	HCR	580:582	HCR	580:582	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	3	23	theme	hybridization	550:562	arg1	reaction					570:577	the proximity-induced hybridization chain reaction	528:577	the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo	528:656	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	8	24	theme	high	1266:1269	arg1	contrast					1271:1278	the high contrast	1262:1278	the high contrast	1262:1278	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	6	25	theme	protein	1006:1012	arg1	probes					1014:1019	the protein probes	1002:1019	the protein probes	1002:1019	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	8	26	theme	imaging	1318:1324	arg1	penetration					1289:1299	deep penetration	1284:1299	deep penetration of photoacoustic imaging	1284:1324	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	8	26	theme	imaging	1318:1324	arg1	contrast					1271:1278	the high contrast	1262:1278	the high contrast	1262:1278	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	3	27	theme	chain	564:568	arg1	HCR					580:582	HCR	580:582	HCR	580:582	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	3	27	theme	chain	564:568	arg1	reaction					570:577	the proximity-induced hybridization chain reaction	528:577	the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo	528:656	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	0	28	theme	Hybridization	18:30	arg1	Chain					32:36	Proximity-Induced Hybridization Chain	0:36	Proximity-Induced Hybridization Chain	0:36	Proximity-Induced Hybridization Chain Reaction-Based Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice.					
34143599	6	29	from	release	1033:1039	arg1	probes					1014:1019	the protein probes	1002:1019	the protein probes	1002:1019	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	1	30	theme	pathological	233:244	arg1	functions					246:254	several physiological and pathological functions	207:254	several physiological and pathological functions	207:254	Glycosylation is a key cellular mechanism that regulates several physiological and pathological functions.					
34143599	0	31	theme	Proximity-Induced	0:16	arg1	Chain					32:36	Proximity-Induced Hybridization Chain	0:36	Proximity-Induced Hybridization Chain	0:36	Proximity-Induced Hybridization Chain Reaction-Based Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice.					
34143599	4	32	theme	functional	672:681	arg1	probes					687:692	functional DNA probes	672:692	functional DNA probes	672:692	Two kinds of functional DNA probes were designed.					
34143599	8	33	dep	in	1349:1350	arg1	situ					1352:1355	situ	1352:1355	situ	1352:1355	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	3	34	theme	proximity-induced	532:548	arg1	HCR					580:582	HCR	580:582	HCR	580:582	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	3	34	theme	proximity-induced	532:548	arg1	reaction					570:577	the proximity-induced hybridization chain reaction	528:577	the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo	528:656	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	8	35	theme	-specific	1384:1392	arg1	glycosylation					1394:1406	Mucin 1 (MUC1)-specific glycosylation	1370:1406	Mucin 1 (MUC1)-specific glycosylation	1370:1406	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	9	36	from	role	1673:1676	arg1	processes					1689:1697	disease processes	1681:1697	disease processes	1681:1697	This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.					
34143599	6	37	theme	Proximity-induced	875:891	arg1	hybridization					893:905	Proximity-induced hybridization	875:905	Proximity-induced hybridization of the complementary domain between the two kinds of probes	875:965	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	9	38	dep	structure	1620:1628	arg1	the					1608:1610	the	1608:1610	the	1608:1610	This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.					
34143599	8	39	theme	tunicamycin	1488:1498	arg1	treatment					1500:1508	tunicamycin treatment	1488:1508	tunicamycin treatment	1488:1508	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	3	40	theme	photoacoustic	490:502	arg1	nanoprobe					509:517	a photoacoustic (PA) nanoprobe	488:517	a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo	488:656	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	4	41	theme	probes	687:692	arg1	kinds					663:667	Two kinds	659:667	Two kinds of functional DNA probes	659:692	Two kinds of functional DNA probes were designed.					
34143599	5	42	theme	protein	814:820	arg1	PP					829:830	PP	829:830	PP	829:830	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	5	42	theme	protein	814:820	arg1	probe					822:826	protein probe	814:826	protein probe (PP)	814:831	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	0	43	from	Glycosylation	127:139	arg1	Mice					144:147	Mice	144:147	Mice	144:147	Proximity-Induced Hybridization Chain Reaction-Based Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice.					
34143599	2	44	theme	glycosylation-related	380:400	arg1	pathology					402:410	glycosylation-related pathology	380:410	glycosylation-related pathology	380:410	Therefore, identification and characterization of specific-protein glycosylation in vivo are highly desirable for studying glycosylation-related pathology and developing personalized theranostic modalities.					
34143599	7	45	theme	Gold	1070:1073	arg1	AuNPs					1090:1094	AuNPs	1090:1094	AuNPs	1090:1094	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	7	45	theme	Gold	1070:1073	arg1	nanoparticles					1075:1087	Gold nanoparticles	1070:1087	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2)	1070:1149	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	0	46	theme	Imaging	67:73	arg1	System					75:80	Photoacoustic Imaging System	53:80	Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice	53:147	Proximity-Induced Hybridization Chain Reaction-Based Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice.					
34143599	4	47	theme	DNA	683:685	arg1	probes					687:692	functional DNA probes	672:692	functional DNA probes	672:692	Two kinds of functional DNA probes were designed.					
34143599	2	48	theme	specific-protein	307:322	arg1	glycosylation					324:336	specific-protein glycosylation	307:336	specific-protein glycosylation	307:336	Therefore, identification and characterization of specific-protein glycosylation in vivo are highly desirable for studying glycosylation-related pathology and developing personalized theranostic modalities.					
34143599	8	49	theme	in	1349:1350	arg1	detection					1357:1365	in situ detection	1349:1365	in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer	1349:1433	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	0	50	theme	Photoacoustic	53:65	arg1	System					75:80	Photoacoustic Imaging System	53:80	Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice	53:147	Proximity-Induced Hybridization Chain Reaction-Based Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice.					
34143599	6	51	theme	domain	1062:1067	arg1	release					1033:1039	in situ release	1025:1039	in situ release of the HCR initiator domain	1025:1067	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	6	51	theme	domain	1062:1067	arg1	changes					991:997	conformational changes	976:997	conformational changes in the protein probes	976:1019	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	7	52	theme	Au	1171:1172	arg1	aggregates					1174:1183	Au aggregates	1171:1183	Au aggregates	1171:1183	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	6	53	theme	conformational	976:989	arg1	changes					991:997	conformational changes	976:997	conformational changes in the protein probes	976:1019	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	9	54	from	effects	1580:1586	arg1	function					1634:1641	function	1634:1641	function	1634:1641	This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.					
34143599	9	54	from	effects	1580:1586	arg1	structure					1620:1628	protein structure	1612:1628	protein structure	1612:1628	This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.					
34143599	8	55	theme	deep	1284:1287	arg1	penetration					1289:1299	deep penetration	1284:1299	deep penetration of photoacoustic imaging	1284:1324	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	7	56	theme	photoacoustic	1232:1244	arg1	signals					1246:1252	photoacoustic signals	1232:1252	photoacoustic signals	1232:1252	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	6	57	theme	initiator	1052:1060	arg1	domain					1062:1067	the HCR initiator domain	1044:1067	the HCR initiator domain	1044:1067	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	8	58	theme	photoacoustic	1304:1316	arg1	imaging					1318:1324	photoacoustic imaging	1304:1324	photoacoustic imaging	1304:1324	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	5	59	theme	aptamer	854:860	arg1	recognition					862:872	aptamer recognition	854:872	aptamer recognition	854:872	A glycan probe (DBCO-GP) was attached to glycans through metabolic oligosaccharide engineering (MOE) and protein probe (PP)-targeted proteins by aptamer recognition.					
34143599	7	60	theme	complementary	1109:1121	arg1	sequences					1123:1131	complementary sequences	1109:1131	complementary sequences (Au-H1 and Au-H2)	1109:1149	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	7	60	theme	complementary	1109:1121	arg1	Au-H2					1144:1148	Au-H2	1144:1148	Au-H2	1144:1148	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	7	60	theme	complementary	1109:1121	arg1	Au-H1					1134:1138	Au-H1	1134:1138	Au-H1	1134:1138	Gold nanoparticles (AuNPs) modified by complementary sequences (Au-H1 and Au-H2) self-assembled into Au aggregates via the HCR, thereby converting DNA signals to photoacoustic signals.					
34143599	3	61	theme	visual	599:604	arg1	detection					606:614	amplified visual detection	589:614	amplified visual detection of protein-specific glycosylation in vivo	589:656	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	9	62	theme	protein	1612:1618	arg1	structure					1620:1628	protein structure	1612:1628	protein structure	1612:1628	This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.					
34143599	6	63	theme	HCR	1048:1050	arg1	domain					1062:1067	the HCR initiator domain	1044:1067	the HCR initiator domain	1044:1067	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	6	64	theme	probes	960:965	arg1	kinds					951:955	the two kinds	943:955	the two kinds of probes	943:965	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	3	65	theme	protein-specific	619:634	arg1	glycosylation					636:648	protein-specific glycosylation	619:648	protein-specific glycosylation in vivo	619:656	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	8	66	with	mice	1411:1414	arg1	cancer					1428:1433	breast cancer	1421:1433	breast cancer	1421:1433	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
34143599	1	67	theme	key	169:171	arg1	mechanism					182:190	a key cellular mechanism	167:190	a key cellular mechanism that regulates several physiological and pathological functions	167:254	Glycosylation is a key cellular mechanism that regulates several physiological and pathological functions.					
34143599	1	67	theme	key	169:171	arg1	Glycosylation					150:162	Glycosylation	150:162	Glycosylation	150:162	Glycosylation is a key cellular mechanism that regulates several physiological and pathological functions.					
34143599	6	68	from	changes	991:997	arg1	probes					1014:1019	the protein probes	1002:1019	the protein probes	1002:1019	Proximity-induced hybridization of the complementary domain between the two kinds of probes promoted conformational changes in the protein probes and in situ release of the HCR initiator domain.					
34143599	9	69	theme	powerful	1545:1552	arg1	platform					1554:1561	a powerful platform	1543:1561	a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes	1543:1697	This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.					
34143599	9	70	theme	imaging	1516:1522	arg1	technique					1524:1532	This imaging technique	1511:1532	This imaging technique	1511:1532	This imaging technique provides a powerful platform for studying the effects of glycosylation on the protein structure and function, which helps to elucidate its role in disease processes.					
34143599	1	71	theme	cellular	173:180	arg1	mechanism					182:190	a key cellular mechanism	167:190	a key cellular mechanism that regulates several physiological and pathological functions	167:254	Glycosylation is a key cellular mechanism that regulates several physiological and pathological functions.					
34143599	1	71	theme	cellular	173:180	arg1	Glycosylation					150:162	Glycosylation	150:162	Glycosylation	150:162	Glycosylation is a key cellular mechanism that regulates several physiological and pathological functions.					
34143599	2	72	theme	theranostic	440:450	arg1	modalities					452:461	personalized theranostic modalities	427:461	personalized theranostic modalities	427:461	Therefore, identification and characterization of specific-protein glycosylation in vivo are highly desirable for studying glycosylation-related pathology and developing personalized theranostic modalities.					
34143599	0	73	theme	Visualization	96:108	arg1	Glycosylation					127:139	Amplified Visualization Protein-Specific Glycosylation	86:139	Amplified Visualization Protein-Specific Glycosylation in Mice	86:147	Proximity-Induced Hybridization Chain Reaction-Based Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice.					
34143599	3	74	theme	glycosylation	636:648	arg1	detection					606:614	amplified visual detection	589:614	amplified visual detection of protein-specific glycosylation in vivo	589:656	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	3	75	theme	amplified	589:597	arg1	detection					606:614	amplified visual detection	589:614	amplified visual detection of protein-specific glycosylation in vivo	589:656	Herein, we demonstrated a photoacoustic (PA) nanoprobe based on the proximity-induced hybridization chain reaction (HCR) for amplified visual detection of protein-specific glycosylation in vivo.					
34143599	0	76	theme	Amplified	86:94	arg1	Glycosylation					127:139	Amplified Visualization Protein-Specific Glycosylation	86:139	Amplified Visualization Protein-Specific Glycosylation in Mice	86:147	Proximity-Induced Hybridization Chain Reaction-Based Photoacoustic Imaging System for Amplified Visualization Protein-Specific Glycosylation in Mice.					
34143599	2	77	theme	glycosylation	324:336	arg1	characterization					287:302	characterization	287:302	characterization of specific-protein glycosylation in vivo	287:344	Therefore, identification and characterization of specific-protein glycosylation in vivo are highly desirable for studying glycosylation-related pathology and developing personalized theranostic modalities.					
34143599	2	77	theme	glycosylation	324:336	arg1	identification					268:281	identification	268:281	identification	268:281	Therefore, identification and characterization of specific-protein glycosylation in vivo are highly desirable for studying glycosylation-related pathology and developing personalized theranostic modalities.					
34143599	8	78	theme	glycosylation	1394:1406	arg1	detection					1357:1365	in situ detection	1349:1365	in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer	1349:1433	Due to the high contrast and deep penetration of photoacoustic imaging, this strategy enabled in situ detection of Mucin 1 (MUC1)-specific glycosylation in mice with breast cancer and successfully monitored its dynamic states during tunicamycin treatment.					
33310702	7	0	theme	Gene	1129:1132	arg1	profiling					1145:1153	Gene expression profiling	1129:1153	Gene expression profiling by RNA-Seq and qRT-PCR	1129:1176	Gene expression profiling by RNA-Seq and qRT-PCR revealed expression levels for glycan-modifying genes that correlated with CD16a glycan composition.					
33310702	2	1	theme	killer	363:368	arg1	cells					375:379	natural killer (NK) cells	355:379	natural killer (NK) cells	355:379	Preeminent among the five activating human FcγRs is FcγRIIIa/CD16a expressed by natural killer (NK) cells.					
33310702	6	2	theme	processing	916:925	arg1	differences					927:937	substantial processing differences	904:937	substantial processing differences using a glycoproteomics approach	904:970	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	1	3	theme	binding	238:244	arg1	affinity					246:253	the binding affinity	234:253	the binding affinity	234:253	Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the binding affinity increases efficacy.					
33310702	0	4	theme	genes	85:89	arg1	the expression					52:65	the expression	52:65	the expression of glycan-related genes in human natural killer cells	52:119	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	7	5	theme	expression	1134:1143	arg1	profiling					1145:1153	Gene expression profiling	1129:1153	Gene expression profiling by RNA-Seq and qRT-PCR	1129:1176	Gene expression profiling by RNA-Seq and qRT-PCR revealed expression levels for glycan-modifying genes that correlated with CD16a glycan composition.					
33310702	6	6	theme	primary	1111:1117	arg1	cells					1122:1126	primary NK cells	1111:1126	primary NK cells	1111:1126	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	2	7	theme	natural	355:361	arg1	killer					363:368	natural killer	355:368	natural killer (NK) cells	355:379	Preeminent among the five activating human FcγRs is FcγRIIIa/CD16a expressed by natural killer (NK) cells.					
33310702	2	7	theme	natural	355:361	arg1	NK					371:372	NK	371:372	NK	371:372	Preeminent among the five activating human FcγRs is FcγRIIIa/CD16a expressed by natural killer (NK) cells.					
33310702	9	8	theme	CD16a	1540:1544	arg1	processing					1546:1555	CD16a processing	1540:1555	CD16a processing	1540:1555	N-glycan processing correlated with the expression of glycan-modifying genes and thus explained the substantial differences in CD16a processing by NK cells of different origins.					
33310702	0	9	theme	natural	100:106	arg1	cells					115:119	human natural killer cells	94:119	human natural killer cells	94:119	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	8	10	theme	protein	1374:1380	arg1	processing					1345:1354	the processing	1341:1354	the processing of the same human protein by different human cell types	1341:1410	These results identified a high degree of variability between the processing of the same human protein by different human cell types.					
33310702	4	11	theme	specific	573:580	arg1	manipulation					582:593	specific manipulation	573:593	specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells	573:677	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	0	12	theme	human	94:98	arg1	cells					115:119	human natural killer cells	94:119	human natural killer cells	94:119	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	0	13	from	the expression	52:65	arg1	cells					115:119	human natural killer cells	94:119	human natural killer cells	94:119	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	8	14	theme	human	1368:1372	arg1	protein					1374:1380	the same human protein	1359:1380	the same human protein	1359:1380	These results identified a high degree of variability between the processing of the same human protein by different human cell types.					
33310702	8	15	theme	same	1363:1366	arg1	protein					1374:1380	the same human protein	1359:1380	the same human protein	1359:1380	These results identified a high degree of variability between the processing of the same human protein by different human cell types.					
33310702	9	16	theme	glycan-modifying	1467:1482	arg1	genes					1484:1488	glycan-modifying genes	1467:1488	glycan-modifying genes	1467:1488	N-glycan processing correlated with the expression of glycan-modifying genes and thus explained the substantial differences in CD16a processing by NK cells of different origins.					
33310702	6	17	theme	cell	1012:1015	arg1	YTS					1033:1035	YTS	1033:1035	YTS	1033:1035	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	6	17	theme	cell	1012:1015	arg1	NK92					1024:1027	NK92	1024:1027	NK92	1024:1027	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	6	17	theme	cell	1012:1015	arg1	lines					1017:1021	two NK cell lines	1005:1021	two NK cell lines	1005:1021	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	5	18	theme	select	765:770	arg1	genes					772:776	select genes	765:776	select genes that encode processing enzymes in CD16a-expressing effector cells	765:842	However, it is unclear if modifying the expression of select genes that encode processing enzymes in CD16a-expressing effector cells is sufficient to affect N-glycan composition.					
33310702	5	18	theme	select	765:770	arg1	enzymes					801:807	processing enzymes	790:807	processing enzymes in CD16a-expressing effector cells	790:842	However, it is unclear if modifying the expression of select genes that encode processing enzymes in CD16a-expressing effector cells is sufficient to affect N-glycan composition.					
33310702	6	19	theme	substantial	904:914	arg1	differences					927:937	substantial processing differences	904:937	substantial processing differences using a glycoproteomics approach	904:970	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	9	20	theme	substantial	1513:1523	arg1	differences					1525:1535	the substantial differences	1509:1535	the substantial differences in CD16a processing	1509:1555	N-glycan processing correlated with the expression of glycan-modifying genes and thus explained the substantial differences in CD16a processing by NK cells of different origins.					
33310702	3	21	theme	carbohydrates	498:510	arg1	composition					447:457	the composition	443:457	the composition of the five CD16a asparagine(N)-linked carbohydrates (glycans)	443:520	CD16a is heavily processed, and recent reports indicate that the composition of the five CD16a asparagine(N)-linked carbohydrates (glycans) impacts affinity.					
33310702	1	22	theme	Fc	180:181	arg1	receptors					185:193	Fc γ receptors	180:193	Fc γ receptors (FcγRs)	180:201	Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the binding affinity increases efficacy.					
33310702	1	22	theme	Fc	180:181	arg1	FcγRs					196:200	FcγRs	196:200	FcγRs	196:200	Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the binding affinity increases efficacy.					
33310702	0	23	theme	killer	108:113	arg1	cells					115:119	human natural killer cells	94:119	human natural killer cells	94:119	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	5	24	from	enzymes	801:807	arg1	cells					838:842	CD16a-expressing effector cells	812:842	CD16a-expressing effector cells	812:842	However, it is unclear if modifying the expression of select genes that encode processing enzymes in CD16a-expressing effector cells is sufficient to affect N-glycan composition.					
33310702	9	25	theme	NK	1560:1561	arg1	cells					1563:1567	NK cells	1560:1567	NK cells of different origins	1560:1588	N-glycan processing correlated with the expression of glycan-modifying genes and thus explained the substantial differences in CD16a processing by NK cells of different origins.					
33310702	6	26	theme	CD16a	1100:1104	arg1	HEK293F cells					1062:1074	HEK293F cells	1062:1074	HEK293F cells	1062:1074	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	6	26	theme	CD16a	1100:1104	arg1	reports					1089:1095	previous reports	1080:1095	previous reports of CD16a from primary NK cells	1080:1126	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	1	27	theme	γ	183:183	arg1	receptors					185:193	Fc γ receptors	180:193	Fc γ receptors (FcγRs)	180:201	Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the binding affinity increases efficacy.					
33310702	1	27	theme	γ	183:183	arg1	FcγRs					196:200	FcγRs	196:200	FcγRs	196:200	Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the binding affinity increases efficacy.					
33310702	0	28	theme	γ	3:3	arg1	receptor					5:12	Fc γ receptor	0:12	Fc γ receptor IIIa/CD16a processing	0:34	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	4	29	from	manipulation	582:593	arg1	cells					654:658	CD16a-expressing effector cells	628:658	CD16a-expressing effector cells including NK cells	628:677	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	4	29	from	manipulation	582:593	arg1	cells					673:677	NK cells	670:677	NK cells	670:677	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	9	30	theme	different	1572:1580	arg1	origins					1582:1588	different origins	1572:1588	different origins	1572:1588	N-glycan processing correlated with the expression of glycan-modifying genes and thus explained the substantial differences in CD16a processing by NK cells of different origins.					
33310702	8	31	theme	cell	1401:1404	arg1	types					1406:1410	different human cell types	1385:1410	different human cell types	1385:1410	These results identified a high degree of variability between the processing of the same human protein by different human cell types.					
33310702	7	32	theme	expression	1187:1196	arg1	levels					1198:1203	expression levels	1187:1203	expression levels for glycan-modifying genes that correlated with CD16a glycan composition	1187:1276	Gene expression profiling by RNA-Seq and qRT-PCR revealed expression levels for glycan-modifying genes that correlated with CD16a glycan composition.					
33310702	6	33	theme	previous	1080:1087	arg1	reports					1089:1095	previous reports	1080:1095	previous reports of CD16a from primary NK cells	1080:1126	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	0	34	theme	Fc	0:1	arg1	receptor					5:12	Fc γ receptor	0:12	Fc γ receptor IIIa/CD16a processing	0:34	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	6	35	theme	NK	1009:1010	arg1	YTS					1033:1035	YTS	1033:1035	YTS	1033:1035	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	6	35	theme	NK	1009:1010	arg1	NK92					1024:1027	NK92	1024:1027	NK92	1024:1027	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	6	35	theme	NK	1009:1010	arg1	lines					1017:1021	two NK cell lines	1005:1021	two NK cell lines	1005:1021	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	2	36	theme	human	312:316	arg1	FcγRs					318:322	the five activating human FcγRs	292:322	the five activating human FcγRs	292:322	Preeminent among the five activating human FcγRs is FcγRIIIa/CD16a expressed by natural killer (NK) cells.					
33310702	3	37	theme	recent	414:419	arg1	reports					421:427	recent reports	414:427	recent reports	414:427	CD16a is heavily processed, and recent reports indicate that the composition of the five CD16a asparagine(N)-linked carbohydrates (glycans) impacts affinity.					
33310702	0	38	theme	IIIa/CD16a	14:23	arg1	processing					25:34	Fc γ receptor IIIa/CD16a processing	0:34	Fc γ receptor IIIa/CD16a processing	0:34	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	2	39	theme	activating	301:310	arg1	FcγRs					318:322	the five activating human FcγRs	292:322	the five activating human FcγRs	292:322	Preeminent among the five activating human FcγRs is FcγRIIIa/CD16a expressed by natural killer (NK) cells.					
33310702	0	40	theme	receptor	5:12	arg1	processing					25:34	Fc γ receptor IIIa/CD16a processing	0:34	Fc γ receptor IIIa/CD16a processing	0:34	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	8	41	theme	different	1385:1393	arg1	types					1406:1410	different human cell types	1385:1410	different human cell types	1385:1410	These results identified a high degree of variability between the processing of the same human protein by different human cell types.					
33310702	4	42	theme	treatment	691:699	arg1	efficacy					701:708	treatment efficacy	691:708	treatment efficacy	691:708	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	9	43	theme	origins	1582:1588	arg1	cells					1563:1567	NK cells	1560:1567	NK cells of different origins	1560:1588	N-glycan processing correlated with the expression of glycan-modifying genes and thus explained the substantial differences in CD16a processing by NK cells of different origins.					
33310702	4	44	theme	composition	613:623	arg1	manipulation					582:593	specific manipulation	573:593	specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells	573:677	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	5	45	theme	genes	772:776	arg1	expression					751:760	the expression	747:760	the expression of select genes that encode processing enzymes in CD16a-expressing effector cells	747:842	However, it is unclear if modifying the expression of select genes that encode processing enzymes in CD16a-expressing effector cells is sufficient to affect N-glycan composition.					
33310702	0	46	from	genes	85:89	arg1	cells					115:119	human natural killer cells	94:119	human natural killer cells	94:119	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	4	47	theme	NK	670:671	arg1	cells					673:677	NK cells	670:677	NK cells	670:677	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	4	48	theme	effector	645:652	arg1	cells					654:658	CD16a-expressing effector cells	628:658	CD16a-expressing effector cells including NK cells	628:677	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	4	48	theme	effector	645:652	arg1	cells					673:677	NK cells	670:677	NK cells	670:677	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	9	49	theme	genes	1484:1488	arg1	expression					1453:1462	the expression	1449:1462	the expression of glycan-modifying genes	1449:1488	N-glycan processing correlated with the expression of glycan-modifying genes and thus explained the substantial differences in CD16a processing by NK cells of different origins.					
33310702	3	50	link	-linked	490:496	arg1	glycans					513:519	glycans	513:519	glycans	513:519	CD16a is heavily processed, and recent reports indicate that the composition of the five CD16a asparagine(N)-linked carbohydrates (glycans) impacts affinity.					
33310702	3	50	link	-linked	490:496	arg1	carbohydrates					498:510	the five CD16a asparagine(N)-linked carbohydrates	462:510	the five CD16a asparagine(N)-linked carbohydrates (glycans)	462:520	CD16a is heavily processed, and recent reports indicate that the composition of the five CD16a asparagine(N)-linked carbohydrates (glycans) impacts affinity.					
33310702	5	51	theme	CD16a-expressing	812:827	arg1	cells					838:842	CD16a-expressing effector cells	812:842	CD16a-expressing effector cells	812:842	However, it is unclear if modifying the expression of select genes that encode processing enzymes in CD16a-expressing effector cells is sufficient to affect N-glycan composition.					
33310702	9	52	from	differences	1525:1535	arg1	processing					1546:1555	CD16a processing	1540:1555	CD16a processing	1540:1555	N-glycan processing correlated with the expression of glycan-modifying genes and thus explained the substantial differences in CD16a processing by NK cells of different origins.					
33310702	1	53	theme	full	207:210	arg1	effect					212:217	full effect	207:217	full effect	207:217	Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the binding affinity increases efficacy.					
33310702	6	54	from	cells	1122:1126	arg1	HEK293F cells					1062:1074	HEK293F cells	1062:1074	HEK293F cells	1062:1074	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	6	54	from	cells	1122:1126	arg1	reports					1089:1095	previous reports	1080:1095	previous reports of CD16a from primary NK cells	1080:1126	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	8	55	theme	variability	1321:1331	arg1	degree					1311:1316	a high degree	1304:1316	a high degree of variability between the processing of the same human protein by different human cell types	1304:1410	These results identified a high degree of variability between the processing of the same human protein by different human cell types.					
33310702	5	56	theme	processing	790:799	arg1	genes					772:776	select genes	765:776	select genes that encode processing enzymes in CD16a-expressing effector cells	765:842	However, it is unclear if modifying the expression of select genes that encode processing enzymes in CD16a-expressing effector cells is sufficient to affect N-glycan composition.					
33310702	5	56	theme	processing	790:799	arg1	enzymes					801:807	processing enzymes	790:807	processing enzymes in CD16a-expressing effector cells	790:842	However, it is unclear if modifying the expression of select genes that encode processing enzymes in CD16a-expressing effector cells is sufficient to affect N-glycan composition.					
33310702	7	57	theme	glycan-modifying	1209:1224	arg1	genes					1226:1230	glycan-modifying genes	1209:1230	glycan-modifying genes that correlated with CD16a glycan composition	1209:1276	Gene expression profiling by RNA-Seq and qRT-PCR revealed expression levels for glycan-modifying genes that correlated with CD16a glycan composition.					
33310702	0	58	from	cells	115:119	arg1	the expression					52:65	the expression	52:65	the expression of glycan-related genes in human natural killer cells	52:119	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	1	59	theme	Many	122:125	arg1	antibodies					150:159	Many therapeutic monoclonal antibodies	122:159	Many therapeutic monoclonal antibodies	122:159	Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the binding affinity increases efficacy.					
33310702	8	60	theme	human	1395:1399	arg1	types					1406:1410	different human cell types	1385:1410	different human cell types	1385:1410	These results identified a high degree of variability between the processing of the same human protein by different human cell types.					
33310702	1	61	theme	therapeutic	127:137	arg1	antibodies					150:159	Many therapeutic monoclonal antibodies	122:159	Many therapeutic monoclonal antibodies	122:159	Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the binding affinity increases efficacy.					
33310702	9	62	theme	N-glycan	1413:1420	arg1	processing					1422:1431	N-glycan processing	1413:1431	N-glycan processing	1413:1431	N-glycan processing correlated with the expression of glycan-modifying genes and thus explained the substantial differences in CD16a processing by NK cells of different origins.					
33310702	8	63	theme	high	1306:1309	arg1	degree					1311:1316	a high degree	1304:1316	a high degree of variability between the processing of the same human protein by different human cell types	1304:1410	These results identified a high degree of variability between the processing of the same human protein by different human cell types.					
33310702	1	64	theme	monoclonal	139:148	arg1	antibodies					150:159	Many therapeutic monoclonal antibodies	122:159	Many therapeutic monoclonal antibodies	122:159	Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the binding affinity increases efficacy.					
33310702	0	65	theme	glycan-related	70:83	arg1	genes					85:89	glycan-related genes	70:89	glycan-related genes in human natural killer cells	70:119	Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.					
33310702	4	66	theme	CD16a-expressing	628:643	arg1	cells					654:658	CD16a-expressing effector cells	628:658	CD16a-expressing effector cells including NK cells	628:677	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	4	66	theme	CD16a-expressing	628:643	arg1	cells					673:677	NK cells	670:677	NK cells	670:677	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	6	67	attach	isolated	991:998	arg1	YTS					1033:1035	YTS	1033:1035	YTS	1033:1035	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	6	67	attach	isolated	991:998	arg1	NK92					1024:1027	NK92	1024:1027	NK92	1024:1027	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	6	67	attach	isolated	991:998	arg2	CD16a					985:989	CD16a	985:989	CD16a isolated from two NK cell lines, NK92 and YTS	985:1035	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	6	67	attach	isolated	991:998	arg1	lines					1017:1021	two NK cell lines	1005:1021	two NK cell lines	1005:1021	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	6	68	theme	NK	1119:1120	arg1	cells					1122:1126	primary NK cells	1111:1126	primary NK cells	1111:1126	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	5	69	theme	N-glycan	868:875	arg1	composition					877:887	N-glycan composition	868:887	N-glycan composition	868:887	However, it is unclear if modifying the expression of select genes that encode processing enzymes in CD16a-expressing effector cells is sufficient to affect N-glycan composition.					
33310702	4	70	theme	N-glycan	604:611	arg1	composition					613:623	CD16a N-glycan composition	598:623	CD16a N-glycan composition	598:623	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
33310702	7	71	theme	glycan	1259:1264	arg1	composition					1266:1276	glycan composition	1259:1276	glycan composition	1259:1276	Gene expression profiling by RNA-Seq and qRT-PCR revealed expression levels for glycan-modifying genes that correlated with CD16a glycan composition.					
33310702	6	72	theme	glycoproteomics	947:961	arg1	approach					963:970	a glycoproteomics approach	945:970	a glycoproteomics approach	945:970	We identified substantial processing differences using a glycoproteomics approach by comparing CD16a isolated from two NK cell lines, NK92 and YTS, with CD16a expressed by HEK293F cells and previous reports of CD16a from primary NK cells.					
33310702	5	73	theme	effector	829:836	arg1	cells					838:842	CD16a-expressing effector cells	812:842	CD16a-expressing effector cells	812:842	However, it is unclear if modifying the expression of select genes that encode processing enzymes in CD16a-expressing effector cells is sufficient to affect N-glycan composition.					
33310702	3	74	theme	-linked	490:496	arg1	glycans					513:519	glycans	513:519	glycans	513:519	CD16a is heavily processed, and recent reports indicate that the composition of the five CD16a asparagine(N)-linked carbohydrates (glycans) impacts affinity.					
33310702	3	74	theme	-linked	490:496	arg1	carbohydrates					498:510	the five CD16a asparagine(N)-linked carbohydrates	462:510	the five CD16a asparagine(N)-linked carbohydrates (glycans)	462:520	CD16a is heavily processed, and recent reports indicate that the composition of the five CD16a asparagine(N)-linked carbohydrates (glycans) impacts affinity.					
33310702	4	75	theme	CD16a	598:602	arg1	composition					613:623	CD16a N-glycan composition	598:623	CD16a N-glycan composition	598:623	These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy.					
32470085	4	0	theme	serine	805:810	arg1	C1s					798:800	C1s	798:800	C1s	798:800	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	4	0	theme	serine	805:810	arg1	protease					812:819	a serine protease	803:819	a serine protease in the complement pathway	803:845	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	10	1	theme	CHO	1716:1718	arg1	cells					1720:1724	CHO cells	1716:1724	CHO cells	1716:1724	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	3	2	theme	previous	487:494	arg1	studies					496:502	previous studies	487:502	previous studies	487:502	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	9	3	theme	glycosylation	1474:1486	arg1	pathway					1488:1494	the N-linked glycosylation pathway	1461:1494	the N-linked glycosylation pathway	1461:1494	A1, contains a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway.					
32470085	10	4	theme	Factor	1609:1614	arg1	VIII					1616:1619	human Factor VIII	1603:1619	human Factor VIII	1603:1619	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	10	4	theme	Factor	1609:1614	arg1	proteins					1638:1645	two recombinant proteins	1622:1645	two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells	1622:1724	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	4	5	theme	stable	985:990	arg1	lines					1003:1007	stable CHO-S cell lines	985:1007	stable CHO-S cell lines	985:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	10	6	dep	proteolysis	1675:1685	arg1	clipping					1688:1695	clipping	1688:1695	clipping	1688:1695	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	12	7	theme	anti-retroviral	1995:2009	arg1	drugs					2011:2015	anti-retroviral drugs	1995:2015	anti-retroviral drugs (elite neutralizer/controller phenotypes)	1995:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	12	7	theme	anti-retroviral	1995:2009	arg1	phenotypes					2047:2056	elite neutralizer/controller phenotypes	2018:2056	elite neutralizer/controller phenotypes	2018:2056	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	3	8	theme	recombinant	525:535	arg1	proteins					541:548	recombinant Env proteins	525:548	recombinant Env proteins from clade B viruses	525:569	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	3	8	theme	recombinant	525:535	arg1	subtype					582:588	the major subtype	572:588	the major subtype represented in North America, Europe, and other parts of the world	572:655	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	1	9	theme	clinical	254:261	arg1	trials					263:268	human clinical trials	248:268	human clinical trials	248:268	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials.					
32470085	11	10	theme	CHOK1	1801:1805	arg1	utility					1755:1761	utility	1755:1761	utility	1755:1761	Finally, we demonstrate the utility and practicality of the C1s-/- MGAT1- CHOK1 1.					
32470085	11	10	theme	CHOK1	1801:1805	arg1	practicality					1767:1778	practicality	1767:1778	practicality	1767:1778	Finally, we demonstrate the utility and practicality of the C1s-/- MGAT1- CHOK1 1.					
32470085	13	11	theme	efficacy	2149:2156	arg1	studies					2158:2164	further immunogenicity and efficacy studies	2122:2164	further immunogenicity and efficacy studies	2122:2164	The Envs represent unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies.					
32470085	10	12	from	specificity	1535:1545	arg1	cleavage					1575:1582	the cleavage	1571:1582	the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells	1571:1724	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	12	13	theme	neutralizing	1921:1932	arg1	antibodies					1934:1943	broadly neutralizing antibodies	1913:1943	broadly neutralizing antibodies	1913:1943	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	5	14	with	lines	1077:1081	arg1	gene					1096:1099	the C1s gene	1088:1099	the C1s gene inactivated by gene editing	1088:1127	In this paper, we describe the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis.					
32470085	3	15	theme	clade	555:559	arg1	viruses					563:569	clade B viruses	555:569	clade B viruses	555:569	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	0	16	theme	proteins	103:110	arg1	Production					76:85	Production	76:85	Production of HIV envelope proteins as vaccine immunogens	76:132	Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens.					
32470085	2	17	theme	biopharmaceutical	285:301	arg1	production					303:312	biopharmaceutical production	285:312	biopharmaceutical production	285:312	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	1	18	theme	envelope	195:202	arg1	glycoproteins					210:222	recombinant envelope (Env) glycoproteins	183:222	recombinant envelope (Env) glycoproteins	183:222	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials.					
32470085	4	19	theme	CHO	866:868	arg1	C1s					798:800	C1s	798:800	C1s	798:800	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	4	19	theme	CHO	866:868	arg1	protease					870:877	the endogenous CHO protease	851:877	the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines	851:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	8	20	theme	cell	1295:1298	arg1	line					1300:1303	The other cell line	1285:1303	The other cell line	1285:1303	The other cell line, C1s-/- MGAT1- CHOK1 1.					
32470085	0	21	theme	vaccine	115:121	arg1	immunogens					123:132	vaccine immunogens	115:132	vaccine immunogens	115:132	Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens.					
32470085	10	22	theme	VIII	1616:1619	arg1	cleavage					1575:1582	the cleavage	1571:1582	the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells	1571:1724	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	1	23	theme	Env	205:207	arg1	glycoproteins					210:222	recombinant envelope (Env) glycoproteins	183:222	recombinant envelope (Env) glycoproteins	183:222	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials.					
32470085	4	24	theme	complement	828:837	arg1	pathway					839:845	the complement pathway	824:845	the complement pathway	824:845	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	10	25	theme	human	1603:1607	arg1	VIII					1616:1619	human Factor VIII	1603:1619	human Factor VIII	1603:1619	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	10	25	theme	human	1603:1607	arg1	proteins					1638:1645	two recombinant proteins	1622:1645	two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells	1622:1724	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	13	26	theme	HIV	2086:2088	arg1	immunogens					2098:2107	unique HIV vaccine immunogens	2079:2107	unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies	2079:2164	The Envs represent unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies.					
32470085	7	27	from	deletion	1259:1266	arg1	gene					1279:1282	the C1s gene	1271:1282	the C1s gene	1271:1282	E7, contains a deletion in the C1s gene.					
32470085	8	28	theme	MGAT1-	1313:1318	arg1	CHOK1					1320:1324	C1s-/- MGAT1- CHOK1 1	1306:1326	C1s-/- MGAT1- CHOK1 1	1306:1326	The other cell line, C1s-/- MGAT1- CHOK1 1.					
32470085	12	29	theme	B	1871:1871	arg1	Envs					1873:1876	clade B Envs	1865:1876	clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1865:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	3	30	theme	present	752:758	arg1	epitopes					743:750	important glycan-dependent epitopes	716:750	important glycan-dependent epitopes present on virions	716:769	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	11	31	theme	C1s-/-	1787:1792	arg1	CHOK1					1801:1805	the C1s-/- MGAT1- CHOK1 1	1783:1807	the C1s-/- MGAT1- CHOK1 1	1783:1807	Finally, we demonstrate the utility and practicality of the C1s-/- MGAT1- CHOK1 1.					
32470085	5	32	theme	C1s	1197:1199	arg1	proteolysis					1201:1211	C1s proteolysis	1197:1211	C1s proteolysis	1197:1211	In this paper, we describe the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis.					
32470085	5	33	theme	susceptible	1182:1192	arg1	protein					1174:1180	any protein	1170:1180	any protein susceptible to C1s proteolysis	1170:1211	In this paper, we describe the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis.					
32470085	3	34	theme	glycan-dependent	726:741	arg1	epitopes					743:750	important glycan-dependent epitopes	716:750	important glycan-dependent epitopes present on virions	716:769	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	1	35	theme	Several	135:141	arg1	vaccines					165:172	Several candidate HIV subunit vaccines	135:172	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins	135:222	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials.					
32470085	2	36	theme	therapeutics	464:475	arg1	manufacturing					422:434	the manufacturing	418:434	the manufacturing of most recombinant protein therapeutics	418:475	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	12	37	theme	clinical	1844:1851	arg1	isolates					1853:1860	clinical isolates	1844:1860	clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1844:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	1	38	gly	glycoproteins	210:222	arg1	glycoproteins					210:222	recombinant envelope (Env) glycoproteins	183:222	recombinant envelope (Env) glycoproteins	183:222	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials.					
32470085	1	39	theme	HIV	153:155	arg1	vaccines					165:172	Several candidate HIV subunit vaccines	135:172	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins	135:222	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials.					
32470085	4	40	theme	responsible	879:889	arg1	C1s					798:800	C1s	798:800	C1s	798:800	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	4	40	theme	responsible	879:889	arg1	protease					870:877	the endogenous CHO protease	851:877	the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines	851:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	12	41	theme	neutralizer/controller	2024:2045	arg1	drugs					2011:2015	anti-retroviral drugs	1995:2015	anti-retroviral drugs (elite neutralizer/controller phenotypes)	1995:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	12	41	theme	neutralizer/controller	2024:2045	arg1	phenotypes					2047:2056	elite neutralizer/controller phenotypes	2018:2056	elite neutralizer/controller phenotypes	2018:2056	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	7	42	contain	contains	1248:1255	arg2	deletion					1259:1266	a deletion	1257:1266	a deletion in the C1s gene	1257:1282	E7, contains a deletion in the C1s gene.					
32470085	7	42	contain	contains	1248:1255	arg1	E7					1244:1245	E7	1244:1245	E7	1244:1245	E7, contains a deletion in the C1s gene.					
32470085	3	43	attach	present	752:758	arg1	virions					763:769	virions	763:769	virions	763:769	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	3	43	attach	present	752:758	arg2	epitopes					743:750	important glycan-dependent epitopes	716:750	important glycan-dependent epitopes present on virions	716:769	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	12	44	from	individuals	1888:1898	arg1	Envs					1873:1876	clade B Envs	1865:1876	clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1865:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	12	44	from	individuals	1888:1898	arg1	isolates					1853:1860	clinical isolates	1844:1860	clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1844:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	2	45	dep	necessary	321:329	arg1	substrate					399:407	the cellular substrate	386:407	the cellular substrate used for the manufacturing of most recombinant protein therapeutics	386:475	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	2	45	dep	necessary	321:329	arg1	it					315:316	it	315:316	it	315:316	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	2	46	theme	Hamster	364:370	arg1	Ovary					372:376	Chinese Hamster Ovary	356:376	Chinese Hamster Ovary	356:376	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	2	46	theme	Hamster	364:370	arg1	CHO					351:353	CHO	351:353	CHO (Chinese Hamster Ovary) cells	351:383	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	0	47	theme	Gene	0:3	arg1	editing					5:11	Gene editing	0:11	Gene editing in CHO cells	0:24	Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens.					
32470085	5	48	theme	lines	1077:1081	arg1	development					1041:1051	the development	1037:1051	the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis	1037:1211	In this paper, we describe the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis.					
32470085	10	49	theme	recombinant	1626:1636	arg1	VIII					1616:1619	human Factor VIII	1603:1619	human Factor VIII	1603:1619	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	10	49	theme	recombinant	1626:1636	arg1	proteins					1638:1645	two recombinant proteins	1622:1645	two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells	1622:1724	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	3	50	from	viruses	563:569	arg1	proteins					541:548	recombinant Env proteins	525:548	recombinant Env proteins from clade B viruses	525:569	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	3	50	from	viruses	563:569	arg1	subtype					582:588	the major subtype	572:588	the major subtype represented in North America, Europe, and other parts of the world	572:655	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	2	51	theme	cellular	390:397	arg1	substrate					399:407	the cellular substrate	386:407	the cellular substrate used for the manufacturing of most recombinant protein therapeutics	386:475	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	2	51	theme	cellular	390:397	arg1	it					315:316	it	315:316	it	315:316	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	8	52	dep	CHOK1	1320:1324	arg1	line					1300:1303	The other cell line	1285:1303	The other cell line	1285:1303	The other cell line, C1s-/- MGAT1- CHOK1 1.					
32470085	2	53	theme	recombinant	444:454	arg1	therapeutics					464:475	most recombinant protein therapeutics	439:475	most recombinant protein therapeutics	439:475	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	10	54	theme	unintended	1664:1673	arg1	proteolysis					1675:1685	unintended proteolysis	1664:1685	unintended proteolysis (clipping)	1664:1696	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	2	55	theme	most	439:442	arg1	therapeutics					464:475	most recombinant protein therapeutics	439:475	most recombinant protein therapeutics	439:475	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	0	56	dep	prevent	29:35	arg1	Production					76:85	Production	76:85	Production of HIV envelope proteins as vaccine immunogens	76:132	Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens.					
32470085	9	57	link	N-linked	1465:1472	arg1	pathway					1488:1494	the N-linked glycosylation pathway	1461:1494	the N-linked glycosylation pathway	1461:1494	A1, contains a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway.					
32470085	4	58	theme	-recombinant	942:953	arg1	rgp120s					963:969	rgp120s	963:969	rgp120s	963:969	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	4	58	theme	-recombinant	942:953	arg1	gp120s					955:960	-recombinant gp120s	942:960	-recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines	942:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	10	59	theme	substrate	1525:1533	arg1	specificity					1535:1545	the substrate specificity	1521:1545	the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells	1521:1724	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	5	60	theme	CHOK1	1066:1070	arg1	lines					1077:1081	two novel CHOK1 cell lines	1056:1081	two novel CHOK1 cell lines	1056:1081	In this paper, we describe the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis.					
32470085	4	61	theme	isolates	930:937	arg1	cleavage					899:906	the cleavage	895:906	the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines	895:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	8	62	theme	other	1289:1293	arg1	line					1300:1303	The other cell line	1285:1303	The other cell line	1285:1303	The other cell line, C1s-/- MGAT1- CHOK1 1.					
32470085	12	63	theme	A1	1809:1810	arg1	line					1817:1820	A1 cell line	1809:1820	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).	1809:2058	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	5	64	theme	gene	1116:1119	arg1	editing					1121:1127	gene editing	1116:1127	gene editing	1116:1127	In this paper, we describe the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis.					
32470085	4	65	theme	B	917:917	arg1	isolates					930:937	clade B laboratory isolates	911:937	clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines	911:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	9	66	theme	N-linked	1465:1472	arg1	pathway					1488:1494	the N-linked glycosylation pathway	1461:1494	the N-linked glycosylation pathway	1461:1494	A1, contains a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway.					
32470085	4	67	theme	CHO-S	992:996	arg1	lines					1003:1007	stable CHO-S cell lines	985:1007	stable CHO-S cell lines	985:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	1	68	theme	human	248:252	arg1	trials					263:268	human clinical trials	248:268	human clinical trials	248:268	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials.					
32470085	9	69	from	deletion	1343:1350	arg1	gene					1387:1390	the MGAT1 gene	1377:1390	the MGAT1 gene	1377:1390	A1, contains a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway.					
32470085	9	69	from	deletion	1343:1350	arg1	gene					1368:1371	the C1s gene	1360:1371	the C1s gene	1360:1371	A1, contains a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway.					
32470085	3	70	theme	CHO	675:677	arg1	cells					679:683	CHO cells	675:683	CHO cells	675:683	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	11	71	theme	MGAT1-	1794:1799	arg1	CHOK1					1801:1805	the C1s-/- MGAT1- CHOK1 1	1783:1807	the C1s-/- MGAT1- CHOK1 1	1783:1807	Finally, we demonstrate the utility and practicality of the C1s-/- MGAT1- CHOK1 1.					
32470085	1	72	theme	subunit	157:163	arg1	vaccines					165:172	Several candidate HIV subunit vaccines	135:172	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins	135:222	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials.					
32470085	6	73	theme	C1s-/-	1229:1234	arg1	CHOK1					1236:1240	C1s-/- CHOK1 2	1229:1242	C1s-/- CHOK1 2	1229:1242	One cell line, C1s-/- CHOK1 2.					
32470085	4	74	theme	clade	911:915	arg1	isolates					930:937	clade B laboratory isolates	911:937	clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines	911:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	11	75	dep	utility	1755:1761	arg1	the					1751:1753	the	1751:1753	the	1751:1753	Finally, we demonstrate the utility and practicality of the C1s-/- MGAT1- CHOK1 1.					
32470085	12	76	contain	possess	1905:1911	arg2	antibodies					1934:1943	broadly neutralizing antibodies	1913:1943	broadly neutralizing antibodies	1913:1943	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	12	76	contain	possess	1905:1911	arg1	individuals					1888:1898	rare individuals	1883:1898	rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1883:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	7	77	theme	C1s	1275:1277	arg1	gene					1279:1282	the C1s gene	1271:1282	the C1s gene	1271:1282	E7, contains a deletion in the C1s gene.					
32470085	3	78	theme	Env	537:539	arg1	proteins					541:548	recombinant Env proteins	525:548	recombinant Env proteins from clade B viruses	525:569	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	3	78	theme	Env	537:539	arg1	subtype					582:588	the major subtype	572:588	the major subtype represented in North America, Europe, and other parts of the world	572:655	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	1	79	theme	recombinant	183:193	arg1	glycoproteins					210:222	recombinant envelope (Env) glycoproteins	183:222	recombinant envelope (Env) glycoproteins	183:222	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials.					
32470085	10	80	theme	rgp120	1592:1597	arg1	cleavage					1575:1582	the cleavage	1571:1582	the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells	1571:1724	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	12	81	theme	Envs	1873:1876	arg1	isolates					1853:1860	clinical isolates	1844:1860	clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1844:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	6	82	theme	cell	1218:1221	arg1	line					1223:1226	One cell line	1214:1226	One cell line	1214:1226	One cell line, C1s-/- CHOK1 2.					
32470085	13	83	theme	further	2122:2128	arg1	studies					2158:2164	further immunogenicity and efficacy studies	2122:2164	further immunogenicity and efficacy studies	2122:2164	The Envs represent unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies.					
32470085	4	84	theme	endogenous	855:864	arg1	C1s					798:800	C1s	798:800	C1s	798:800	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	4	84	theme	endogenous	855:864	arg1	protease					870:877	the endogenous CHO protease	851:877	the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines	851:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	8	85	theme	C1s-/-	1306:1311	arg1	CHOK1					1320:1324	C1s-/- MGAT1- CHOK1 1	1306:1326	C1s-/- MGAT1- CHOK1 1	1306:1326	The other cell line, C1s-/- MGAT1- CHOK1 1.					
32470085	12	86	theme	rare	1883:1886	arg1	individuals					1888:1898	rare individuals	1883:1898	rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1883:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	3	87	theme	important	716:724	arg1	epitopes					743:750	important glycan-dependent epitopes	716:750	important glycan-dependent epitopes present on virions	716:769	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	3	88	theme	B	561:561	arg1	viruses					563:569	clade B viruses	555:569	clade B viruses	555:569	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	13	89	theme	unique	2079:2084	arg1	immunogens					2098:2107	unique HIV vaccine immunogens	2079:2107	unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies	2079:2164	The Envs represent unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies.					
32470085	12	90	theme	isolates	1853:1860	arg1	expression					1830:1839	the expression	1826:1839	the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1826:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	13	91	theme	immunogenicity	2130:2143	arg1	studies					2158:2164	further immunogenicity and efficacy studies	2122:2164	further immunogenicity and efficacy studies	2122:2164	The Envs represent unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies.					
32470085	12	92	from	isolates	1853:1860	arg1	individuals					1888:1898	rare individuals	1883:1898	rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1883:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	13	93	theme	vaccine	2090:2096	arg1	immunogens					2098:2107	unique HIV vaccine immunogens	2079:2107	unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies	2079:2164	The Envs represent unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies.					
32470085	12	94	theme	clade	1865:1869	arg1	Envs					1873:1876	clade B Envs	1865:1876	clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1865:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	5	95	theme	protein	1174:1180	arg1	production					1156:1165	the production	1152:1165	the production of any protein susceptible to C1s proteolysis	1152:1211	In this paper, we describe the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis.					
32470085	3	96	theme	major	576:580	arg1	subtype					582:588	the major subtype	572:588	the major subtype represented in North America, Europe, and other parts of the world	572:655	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	3	96	theme	major	576:580	arg1	proteins					541:548	recombinant Env proteins	525:548	recombinant Env proteins from clade B viruses	525:569	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	13	97	theme	suitable	2109:2116	arg1	immunogens					2098:2107	unique HIV vaccine immunogens	2079:2107	unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies	2079:2164	The Envs represent unique HIV vaccine immunogens suitable for further immunogenicity and efficacy studies.					
32470085	4	98	from	protease	812:819	arg1	pathway					839:845	the complement pathway	824:845	the complement pathway	824:845	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	6	99	dep	CHOK1	1236:1240	arg1	line					1223:1226	One cell line	1214:1226	One cell line	1214:1226	One cell line, C1s-/- CHOK1 2.					
32470085	1	100	theme	candidate	143:151	arg1	vaccines					165:172	Several candidate HIV subunit vaccines	135:172	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins	135:222	Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials.					
32470085	2	101	theme	protein	456:462	arg1	therapeutics					464:475	most recombinant protein therapeutics	439:475	most recombinant protein therapeutics	439:475	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	0	102	from	editing	5:11	arg1	cells					20:24	CHO cells	16:24	CHO cells	16:24	Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens.					
32470085	9	103	theme	C1s	1364:1366	arg1	gene					1368:1371	the C1s gene	1360:1371	the C1s gene	1360:1371	A1, contains a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway.					
32470085	12	104	theme	elite	2018:2022	arg1	drugs					2011:2015	anti-retroviral drugs	1995:2015	anti-retroviral drugs (elite neutralizer/controller phenotypes)	1995:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	12	104	theme	elite	2018:2022	arg1	phenotypes					2047:2056	elite neutralizer/controller phenotypes	2018:2056	elite neutralizer/controller phenotypes	2018:2056	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	0	105	theme	envelope	94:101	arg1	proteins					103:110	HIV envelope proteins	90:110	HIV envelope proteins as vaccine immunogens	90:132	Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens.					
32470085	9	106	theme	MGAT1	1381:1385	arg1	gene					1387:1390	the MGAT1 gene	1377:1390	the MGAT1 gene	1377:1390	A1, contains a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway.					
32470085	4	107	theme	gp120s	955:960	arg1	isolates					930:937	clade B laboratory isolates	911:937	clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines	911:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	2	108	theme	Chinese	356:362	arg1	Ovary					372:376	Chinese Hamster Ovary	356:376	Chinese Hamster Ovary	356:376	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	2	108	theme	Chinese	356:362	arg1	CHO					351:353	CHO	351:353	CHO (Chinese Hamster Ovary) cells	351:383	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32470085	0	109	theme	CHO	16:18	arg1	cells					20:24	CHO cells	16:24	CHO cells	16:24	Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens.					
32470085	9	110	contain	contains	1332:1339	arg1	A1					1328:1329	A1	1328:1329	A1	1328:1329	A1, contains a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway.					
32470085	9	110	contain	contains	1332:1339	arg2	deletion					1343:1350	a deletion	1341:1350	a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway	1341:1494	A1, contains a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway.					
32470085	5	111	theme	C1s	1092:1094	arg1	gene					1096:1099	the C1s gene	1088:1099	the C1s gene inactivated by gene editing	1088:1127	In this paper, we describe the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis.					
32470085	4	112	theme	laboratory	919:928	arg1	isolates					930:937	clade B laboratory isolates	911:937	clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines	911:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	0	113	theme	HIV	90:92	arg1	proteins					103:110	HIV envelope proteins	90:110	HIV envelope proteins as vaccine immunogens	90:132	Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens.					
32470085	5	114	theme	novel	1060:1064	arg1	lines					1077:1081	two novel CHOK1 cell lines	1056:1081	two novel CHOK1 cell lines	1056:1081	In this paper, we describe the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis.					
32470085	3	115	theme	other	632:636	arg1	parts					638:642	other parts	632:642	other parts of the world	632:655	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	12	116	theme	virus	1969:1973	arg1	replication					1975:1985	virus replication	1969:1985	virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes)	1969:2057	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	5	117	theme	cell	1072:1075	arg1	lines					1077:1081	two novel CHOK1 cell lines	1056:1081	two novel CHOK1 cell lines	1056:1081	In this paper, we describe the development of two novel CHOK1 cell lines with the C1s gene inactivated by gene editing, that are suitable for the production of any protein susceptible to C1s proteolysis.					
32470085	4	118	theme	cell	998:1001	arg1	lines					1003:1007	stable CHO-S cell lines	985:1007	stable CHO-S cell lines	985:1007	Previously, we identified C1s, a serine protease in the complement pathway, as the endogenous CHO protease responsible for the cleavage of clade B laboratory isolates of -recombinant gp120s (rgp120s) expressed in stable CHO-S cell lines.					
32470085	9	119	gly	glycosylation	1406:1418	arg1	pathway					1488:1494	the N-linked glycosylation pathway	1461:1494	the N-linked glycosylation pathway	1461:1494	A1, contains a deletion in both the C1s gene and the MGAT1 gene, which limits glycosylation to mannose-5 or earlier intermediates in the N-linked glycosylation pathway.					
32470085	10	120	theme	C1s	1550:1552	arg1	specificity					1535:1545	the substrate specificity	1521:1545	the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells	1521:1724	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	12	121	theme	cell	1812:1815	arg1	line					1817:1820	A1 cell line	1809:1820	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).	1809:2058	A1 cell line for the expression of clinical isolates of clade B Envs from rare individuals that possess broadly neutralizing antibodies and are able to control virus replication without anti-retroviral drugs (elite neutralizer/controller phenotypes).					
32470085	10	122	with	C1s	1550:1552	arg1	thrombin					1559:1566	thrombin	1559:1566	thrombin	1559:1566	In addition, we compare the substrate specificity of C1s with thrombin on the cleavage of both rgp120 and human Factor VIII, two recombinant proteins known to undergo unintended proteolysis (clipping) when expressed in CHO cells.					
32470085	3	123	theme	world	651:655	arg1	Europe					620:625	Europe	620:625	Europe	620:625	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	3	123	theme	world	651:655	arg1	parts					638:642	other parts	632:642	other parts of the world	632:655	However, previous studies have shown that when recombinant Env proteins from clade B viruses, the major subtype represented in North America, Europe, and other parts of the world, are expressed in CHO cells, they are proteolyzed and lack important glycan-dependent epitopes present on virions.					
32470085	2	124	theme	CHO	351:353	arg1	cells					379:383	CHO (Chinese Hamster Ovary) cells	351:383	CHO (Chinese Hamster Ovary) cells	351:383	To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing of most recombinant protein therapeutics.					
32007810	4	0	theme	lower	428:432	arg1	speed					446:450	a lower degradation speed	426:450	a lower degradation speed of moisture and polymers	426:475	The blend films with a higher content of egg white showed a lower degradation speed of moisture and polymers.					
32007810	9	1	theme	electron	1002:1009	arg1	microscopy					1011:1020	scanning electron microscopy	993:1020	the scanning electron microscopy images	989:1027	The apparent color and the scanning electron microscopy images were also assessed in this study.					
32007810	8	2	theme	free	815:818	arg1	content					826:832	free amino content	815:832	free amino content	815:832	The decrease of free amino content indicated that the degree of glycosylation increased with the addition of pullulan, which is associated with the Maillard reaction.					
32007810	5	3	theme	egg	612:614	arg1	structure					599:607	the secondary structure	585:607	the secondary structure of egg white	585:620	FTIR spectroscopy illustrated peak displacement, X-ray diffraction patterns showed noticeable changes, and the secondary structure of egg white was altered.					
32007810	2	4	theme	films	240:244	arg1	properties					216:225	the mechanical properties	201:225	the mechanical properties of the blend films	201:244	As the egg white proportion increased, the mechanical properties of the blend films initially increased and then decreased.					
32007810	4	5	theme	white	413:417	arg1	egg					409:411	egg white	409:417	egg white	409:417	The blend films with a higher content of egg white showed a lower degradation speed of moisture and polymers.					
32007810	5	6	theme	white	616:620	arg1	egg					612:614	egg white	612:620	egg white	612:620	FTIR spectroscopy illustrated peak displacement, X-ray diffraction patterns showed noticeable changes, and the secondary structure of egg white was altered.					
32007810	5	7	theme	noticeable	561:570	arg1	changes					572:578	noticeable changes	561:578	noticeable changes	561:578	FTIR spectroscopy illustrated peak displacement, X-ray diffraction patterns showed noticeable changes, and the secondary structure of egg white was altered.					
32007810	2	8	theme	blend	234:238	arg1	films					240:244	the blend films	230:244	the blend films	230:244	As the egg white proportion increased, the mechanical properties of the blend films initially increased and then decreased.					
32007810	8	9	theme	content	826:832	arg1	decrease					803:810	The decrease	799:810	The decrease of free amino content	799:832	The decrease of free amino content indicated that the degree of glycosylation increased with the addition of pullulan, which is associated with the Maillard reaction.					
32007810	3	10	theme	mechanical	295:304	arg1	properties					306:315	The best mechanical properties	286:315	The best mechanical properties	286:315	The best mechanical properties occurred at the optimal proportion of Pu:EW = 1:1.					
32007810	4	11	theme	egg	409:411	arg1	content					398:404	a higher content	389:404	a higher content of egg white	389:417	The blend films with a higher content of egg white showed a lower degradation speed of moisture and polymers.					
32007810	1	12	theme	Edible	78:83	arg1	films					85:89	Edible films	78:89	Edible films based on pullulan (Pu) and egg white (EW)	78:131	Edible films based on pullulan (Pu) and egg white (EW) were prepared in this study.					
32007810	8	13	theme	amino	820:824	arg1	content					826:832	free amino content	815:832	free amino content	815:832	The decrease of free amino content indicated that the degree of glycosylation increased with the addition of pullulan, which is associated with the Maillard reaction.					
32007810	0	14	theme	egg	47:49	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and film-forming mechanism of egg white/pullulan blend film.					
32007810	0	14	theme	egg	47:49	arg1	mechanism					34:42	film-forming mechanism	21:42	film-forming mechanism	21:42	Characterization and film-forming mechanism of egg white/pullulan blend film.					
32007810	8	15	theme	Maillard	947:954	arg1	reaction					956:963	the Maillard reaction	943:963	the Maillard reaction	943:963	The decrease of free amino content indicated that the degree of glycosylation increased with the addition of pullulan, which is associated with the Maillard reaction.					
32007810	5	16	theme	secondary	589:597	arg1	structure					599:607	the secondary structure	585:607	the secondary structure of egg white	585:620	FTIR spectroscopy illustrated peak displacement, X-ray diffraction patterns showed noticeable changes, and the secondary structure of egg white was altered.					
32007810	5	17	theme	X-ray	527:531	arg1	patterns					545:552	X-ray diffraction patterns	527:552	X-ray diffraction patterns	527:552	FTIR spectroscopy illustrated peak displacement, X-ray diffraction patterns showed noticeable changes, and the secondary structure of egg white was altered.					
32007810	7	18	theme	content	790:796	arg1	increment					768:776	the increment	764:776	the increment of pullulan content	764:796	The total α-helix and β-sheet content increased with the increment of pullulan content.					
32007810	5	19	theme	diffraction	533:543	arg1	patterns					545:552	X-ray diffraction patterns	527:552	X-ray diffraction patterns	527:552	FTIR spectroscopy illustrated peak displacement, X-ray diffraction patterns showed noticeable changes, and the secondary structure of egg white was altered.					
32007810	3	20	theme	optimal	333:339	arg1	proportion					341:350	the optimal proportion	329:350	the optimal proportion of Pu:EW = 1:1	329:365	The best mechanical properties occurred at the optimal proportion of Pu:EW = 1:1.					
32007810	4	21	theme	higher	391:396	arg1	content					398:404	a higher content	389:404	a higher content of egg white	389:417	The blend films with a higher content of egg white showed a lower degradation speed of moisture and polymers.					
32007810	8	22	theme	glycosylation	863:875	arg1	degree					853:858	the degree	849:858	the degree of glycosylation	849:875	The decrease of free amino content indicated that the degree of glycosylation increased with the addition of pullulan, which is associated with the Maillard reaction.					
32007810	4	23	with	films	378:382	arg1	content					398:404	a higher content	389:404	a higher content of egg white	389:417	The blend films with a higher content of egg white showed a lower degradation speed of moisture and polymers.					
32007810	3	24	theme	EW = 1:1	358:365	arg1	proportion					341:350	the optimal proportion	329:350	the optimal proportion of Pu:EW = 1:1	329:365	The best mechanical properties occurred at the optimal proportion of Pu:EW = 1:1.					
32007810	9	25	theme	microscopy	1011:1020	arg1	images					1022:1027	the scanning electron microscopy images	989:1027	the scanning electron microscopy images	989:1027	The apparent color and the scanning electron microscopy images were also assessed in this study.					
32007810	7	26	theme	pullulan	781:788	arg1	content					790:796	pullulan content	781:796	pullulan content	781:796	The total α-helix and β-sheet content increased with the increment of pullulan content.					
32007810	7	27	theme	β-sheet	733:739	arg1	content					741:747	The total α-helix and β-sheet content	711:747	The total α-helix and β-sheet content	711:747	The total α-helix and β-sheet content increased with the increment of pullulan content.					
32007810	5	28	theme	FTIR	478:481	arg1	spectroscopy					483:494	FTIR spectroscopy	478:494	FTIR spectroscopy	478:494	FTIR spectroscopy illustrated peak displacement, X-ray diffraction patterns showed noticeable changes, and the secondary structure of egg white was altered.					
32007810	3	29	theme	Pu	355:356	arg1	EW = 1:1					358:365	Pu:EW = 1:1	355:365	Pu:EW = 1:1	355:365	The best mechanical properties occurred at the optimal proportion of Pu:EW = 1:1.					
32007810	4	30	theme	moisture	455:462	arg1	speed					446:450	a lower degradation speed	426:450	a lower degradation speed of moisture and polymers	426:475	The blend films with a higher content of egg white showed a lower degradation speed of moisture and polymers.					
32007810	3	31	theme	best	290:293	arg1	properties					306:315	The best mechanical properties	286:315	The best mechanical properties	286:315	The best mechanical properties occurred at the optimal proportion of Pu:EW = 1:1.					
32007810	2	32	theme	white	173:177	arg1	proportion					179:188	the egg white proportion	165:188	the egg white proportion	165:188	As the egg white proportion increased, the mechanical properties of the blend films initially increased and then decreased.					
32007810	7	33	theme	α-helix	721:727	arg1	content					741:747	The total α-helix and β-sheet content	711:747	The total α-helix and β-sheet content	711:747	The total α-helix and β-sheet content increased with the increment of pullulan content.					
32007810	4	34	theme	blend	372:376	arg1	films					378:382	The blend films	368:382	The blend films with a higher content of egg white	368:417	The blend films with a higher content of egg white showed a lower degradation speed of moisture and polymers.					
32007810	2	35	theme	egg	169:171	arg1	proportion					179:188	the egg white proportion	165:188	the egg white proportion	165:188	As the egg white proportion increased, the mechanical properties of the blend films initially increased and then decreased.					
32007810	9	36	theme	apparent	970:977	arg1	color					979:983	The apparent color	966:983	The apparent color	966:983	The apparent color and the scanning electron microscopy images were also assessed in this study.					
32007810	4	37	theme	polymers	468:475	arg1	speed					446:450	a lower degradation speed	426:450	a lower degradation speed of moisture and polymers	426:475	The blend films with a higher content of egg white showed a lower degradation speed of moisture and polymers.					
32007810	5	38	theme	peak	508:511	arg1	displacement					513:524	peak displacement	508:524	peak displacement	508:524	FTIR spectroscopy illustrated peak displacement, X-ray diffraction patterns showed noticeable changes, and the secondary structure of egg white was altered.					
32007810	2	39	theme	mechanical	205:214	arg1	properties					216:225	the mechanical properties	201:225	the mechanical properties of the blend films	201:244	As the egg white proportion increased, the mechanical properties of the blend films initially increased and then decreased.					
32007810	8	40	theme	pullulan	908:915	arg1	addition					896:903	the addition	892:903	the addition	892:903	The decrease of free amino content indicated that the degree of glycosylation increased with the addition of pullulan, which is associated with the Maillard reaction.					
32007810	7	41	theme	total	715:719	arg1	content					741:747	The total α-helix and β-sheet content	711:747	The total α-helix and β-sheet content	711:747	The total α-helix and β-sheet content increased with the increment of pullulan content.					
32007810	6	42	theme	white	681:685	arg1	egg					677:679	egg white	677:685	egg white	677:685	These results indicated that pullulan and egg white interacted extensively.					
32007810	1	43	theme	white	122:126	arg1	egg					118:120	egg white	118:126	egg white (EW)	118:131	Edible films based on pullulan (Pu) and egg white (EW) were prepared in this study.					
32007810	1	43	theme	white	122:126	arg1	EW					129:130	EW	129:130	EW	129:130	Edible films based on pullulan (Pu) and egg white (EW) were prepared in this study.					
32007810	0	44	theme	blend	66:70	arg1	film					72:75	blend film	66:75	blend film	66:75	Characterization and film-forming mechanism of egg white/pullulan blend film.					
32007810	4	45	theme	degradation	434:444	arg1	speed					446:450	a lower degradation speed	426:450	a lower degradation speed of moisture and polymers	426:475	The blend films with a higher content of egg white showed a lower degradation speed of moisture and polymers.					
32007810	0	46	theme	film-forming	21:32	arg1	mechanism					34:42	film-forming mechanism	21:42	film-forming mechanism	21:42	Characterization and film-forming mechanism of egg white/pullulan blend film.					
32007810	9	47	theme	scanning	993:1000	arg1	microscopy					1011:1020	scanning electron microscopy	993:1020	the scanning electron microscopy images	989:1027	The apparent color and the scanning electron microscopy images were also assessed in this study.					
32636234	5	0	theme	contextual	792:801	arg1	information					803:813	contextual information	792:813	contextual information relative to a set of glycan compositions	792:854	It offers a web interface through which the database can be queried to bring out contextual information relative to a set of glycan compositions.					
32636234	9	1	theme	ExPASy	1350:1355	arg1	initiative					1357:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application.					
32636234	0	2	with	Selection	30:38	arg1	Compozitor					79:88	GlyConnect Compozitor	68:88	GlyConnect Compozitor	68:88	Examining and Fine-tuning the Selection of Glycan Compositions with GlyConnect Compozitor.					
32636234	9	3	theme	tool	1317:1320	arg1	initiative					1357:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application.					
32636234	1	4	theme	theoretical	249:259	arg1	masses					261:266	theoretical masses	249:266	theoretical masses by a glycoproteomics search engine	249:301	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	6	5	theme	monosaccharide	942:955	arg1	counts					957:962	shared monosaccharide counts	935:962	shared monosaccharide counts	935:962	The tool takes advantage of compositions being related to one another through shared monosaccharide counts and outputs interactive graphs summarizing information searched in the database.					
32636234	7	6	theme	study	1164:1168	arg1	context					1151:1157	the context	1147:1157	the context of a study	1147:1168	These results provide a guide for selecting or deselecting compositions in a file in order to reflect the context of a study as closely as possible.					
32636234	9	7	theme	web	1460:1462	arg1	application					1464:1474	a web application	1458:1474	a web application	1458:1474	As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application.					
32636234	4	8	theme	composition	592:602	arg1	visualizing					604:614	a glycan composition visualizing	583:614	a glycan composition visualizing	583:614	We introduce here a glycan composition visualizing and comparative tool associated with the GlyConnect database and called GlyConnect Compozitor.					
32636234	6	9	theme	shared	935:940	arg1	counts					957:962	shared monosaccharide counts	935:962	shared monosaccharide counts	935:962	The tool takes advantage of compositions being related to one another through shared monosaccharide counts and outputs interactive graphs summarizing information searched in the database.					
32636234	4	10	theme	comparative	620:630	arg1	tool					632:635	comparative tool	620:635	comparative tool associated with the GlyConnect database and called GlyConnect Compozitor	620:708	We introduce here a glycan composition visualizing and comparative tool associated with the GlyConnect database and called GlyConnect Compozitor.					
32636234	4	11	theme	GlyConnect	657:666	arg1	database					668:675	the GlyConnect database	653:675	the GlyConnect database	653:675	We introduce here a glycan composition visualizing and comparative tool associated with the GlyConnect database and called GlyConnect Compozitor.					
32636234	9	12	theme	Glycomics	1340:1348	arg1	initiative					1357:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application.					
32636234	10	13	from	database	1528:1535	arg1	accessible					1497:1506	accessible	1497:1506	accessible	1497:1506	It is also directly accessible from the GlyConnect database.					
32636234	9	14	theme	collection	1322:1331	arg1	initiative					1357:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application.					
32636234	3	15	theme	default	494:500	arg1	file					514:517	a default composition file	492:517	a default composition file that is readily used when processing MS data	492:562	Most glycoproteomics search engines include a default composition file that is readily used when processing MS data.					
32636234	3	16	theme	MS	556:557	arg1	data					559:562	processing MS data	545:562	processing MS data	545:562	Most glycoproteomics search engines include a default composition file that is readily used when processing MS data.					
32636234	2	17	theme	querying	382:389	arg1	sources					396:402	the literature and/or querying data sources	360:402	sources	396:402	At present, these files are mainly built from searching the literature and/or querying data sources focused on posttranslational modifications.					
32636234	3	18	theme	glycoproteomics	453:467	arg1	engines					476:482	Most glycoproteomics search engines	448:482	Most glycoproteomics search engines	448:482	Most glycoproteomics search engines include a default composition file that is readily used when processing MS data.					
32636234	8	19	theme	compositions	1240:1251	arg1	compositions					1240:1251	compositions	1240:1251	compositions based on the content of the GlyConnect database	1240:1299	They also confirm the consistency of a set of compositions based on the content of the GlyConnect database.					
32636234	8	19	theme	compositions	1240:1251	arg1	set					1233:1235	a set	1231:1235	a set of compositions based on the content of the GlyConnect database	1231:1299	They also confirm the consistency of a set of compositions based on the content of the GlyConnect database.					
32636234	9	20	theme	of	1333:1334	arg1	initiative					1357:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application.					
32636234	2	21	theme	data	391:394	arg1	sources					396:402	the literature and/or querying data sources	360:402	sources	396:402	At present, these files are mainly built from searching the literature and/or querying data sources focused on posttranslational modifications.					
32636234	4	22	theme	GlyConnect	688:697	arg1	Compozitor					699:708	called GlyConnect Compozitor	681:708	called GlyConnect Compozitor	681:708	We introduce here a glycan composition visualizing and comparative tool associated with the GlyConnect database and called GlyConnect Compozitor.					
32636234	1	23	theme	glycan	185:190	arg1	file					204:207	the glycan composition file	181:207	the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine	181:301	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	5	24	theme	glycan	836:841	arg1	compositions					843:854	glycan compositions	836:854	glycan compositions	836:854	It offers a web interface through which the database can be queried to bring out contextual information relative to a set of glycan compositions.					
32636234	1	25	theme	glycoproteomics	273:287	arg1	engine					296:301	a glycoproteomics search engine	271:301	a glycoproteomics search engine	271:301	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	1	26	theme	key	93:95	arg1	point					97:101	A key point	91:101	A key point in achieving accurate intact glycopeptide identification	91:158	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	1	26	theme	key	93:95	arg1	definition					167:176	the definition	163:176	the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine	163:301	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	1	27	theme	composition	192:202	arg1	file					204:207	the glycan composition file	181:207	the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine	181:301	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	5	28	theme	compositions	843:854	arg1	set					829:831	a set	827:831	a set of glycan compositions	827:854	It offers a web interface through which the database can be queried to bring out contextual information relative to a set of glycan compositions.					
32636234	5	28	theme	compositions	843:854	arg1	compositions					843:854	glycan compositions	836:854	glycan compositions	836:854	It offers a web interface through which the database can be queried to bring out contextual information relative to a set of glycan compositions.					
32636234	1	29	theme	search	289:294	arg1	engine					296:301	a glycoproteomics search engine	271:301	a glycoproteomics search engine	271:301	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	1	30	theme	file	204:207	arg1	point					97:101	A key point	91:101	A key point in achieving accurate intact glycopeptide identification	91:158	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	1	30	theme	file	204:207	arg1	definition					167:176	the definition	163:176	the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine	163:301	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	4	31	theme	glycan	585:590	arg1	visualizing					604:614	a glycan composition visualizing	583:614	a glycan composition visualizing	583:614	We introduce here a glycan composition visualizing and comparative tool associated with the GlyConnect database and called GlyConnect Compozitor.					
32636234	2	32	theme	posttranslational	415:431	arg1	modifications					433:445	posttranslational modifications	415:445	posttranslational modifications	415:445	At present, these files are mainly built from searching the literature and/or querying data sources focused on posttranslational modifications.					
32636234	5	33	theme	relative	815:822	arg1	information					803:813	contextual information	792:813	contextual information relative to a set of glycan compositions	792:854	It offers a web interface through which the database can be queried to bring out contextual information relative to a set of glycan compositions.					
32636234	9	34	theme	initiative	1357:1366	arg1	Compozitor					1369:1378	Compozitor	1369:1378	Compozitor	1369:1378	As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application.					
32636234	9	34	theme	initiative	1357:1366	arg1	part					1305:1308	part	1305:1308	part of the tool collection of the Glycomics@ExPASy initiative	1305:1366	As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application.					
32636234	1	35	gly	glycopeptide	132:143	arg2	glycopeptide					132:143	accurate intact glycopeptide identification	116:158	accurate intact glycopeptide identification	116:158	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	1	36	used	used	217:220	arg2	file					204:207	the glycan composition file	181:207	the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine	181:301	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	3	37	theme	Most	448:451	arg1	engines					476:482	Most glycoproteomics search engines	448:482	Most glycoproteomics search engines	448:482	Most glycoproteomics search engines include a default composition file that is readily used when processing MS data.					
32636234	4	38	theme	called	681:686	arg1	Compozitor					699:708	called GlyConnect Compozitor	681:708	called GlyConnect Compozitor	681:708	We introduce here a glycan composition visualizing and comparative tool associated with the GlyConnect database and called GlyConnect Compozitor.					
32636234	8	39	theme	set	1233:1235	arg1	consistency					1216:1226	the consistency	1212:1226	the consistency of a set of compositions based on the content of the GlyConnect database	1212:1299	They also confirm the consistency of a set of compositions based on the content of the GlyConnect database.					
32636234	8	40	theme	database	1292:1299	arg1	content					1266:1272	the content	1262:1272	the content of the GlyConnect database	1262:1299	They also confirm the consistency of a set of compositions based on the content of the GlyConnect database.					
32636234	0	41	theme	Compositions	50:61	arg1	Selection					30:38	the Selection	26:38	the Selection of Glycan Compositions with GlyConnect Compozitor	26:88	Examining and Fine-tuning the Selection of Glycan Compositions with GlyConnect Compozitor.					
32636234	9	42	theme	@	1349:1349	arg1	initiative					1357:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	the tool collection of the Glycomics@ExPASy initiative	1313:1366	As part of the tool collection of the Glycomics@ExPASy initiative, Compozitor is hosted at https://glyconnect.expasy.org/compozitor/ where it can be run as a web application.					
32636234	10	43	theme	GlyConnect	1517:1526	arg1	database					1528:1535	the GlyConnect database	1513:1535	the GlyConnect database	1513:1535	It is also directly accessible from the GlyConnect database.					
32636234	0	44	theme	Glycan	43:48	arg1	Compositions					50:61	Glycan Compositions	43:61	Glycan Compositions	43:61	Examining and Fine-tuning the Selection of Glycan Compositions with GlyConnect Compozitor.					
32636234	6	45	theme	interactive	976:986	arg1	graphs					988:993	interactive graphs	976:993	interactive graphs summarizing information searched in the database	976:1042	The tool takes advantage of compositions being related to one another through shared monosaccharide counts and outputs interactive graphs summarizing information searched in the database.					
32636234	1	46	theme	accurate	116:123	arg1	identification					145:158	accurate intact glycopeptide identification	116:158	accurate intact glycopeptide identification	116:158	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	5	47	theme	web	723:725	arg1	interface					727:735	a web interface	721:735	a web interface through which the database can be queried to bring out contextual information relative to a set of glycan compositions	721:854	It offers a web interface through which the database can be queried to bring out contextual information relative to a set of glycan compositions.					
32636234	3	48	used	used	535:538	arg2	file					514:517	a default composition file	492:517	a default composition file that is readily used when processing MS data	492:562	Most glycoproteomics search engines include a default composition file that is readily used when processing MS data.					
32636234	1	49	theme	intact	125:130	arg1	identification					145:158	accurate intact glycopeptide identification	116:158	accurate intact glycopeptide identification	116:158	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	6	50	dep	one	915:917	arg1	another					919:925	another	919:925	another	919:925	The tool takes advantage of compositions being related to one another through shared monosaccharide counts and outputs interactive graphs summarizing information searched in the database.					
32636234	1	51	with	experimental	231:242	arg1	masses					261:266	theoretical masses	249:266	theoretical masses by a glycoproteomics search engine	249:301	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	1	52	theme	glycopeptide	132:143	arg1	identification					145:158	accurate intact glycopeptide identification	116:158	accurate intact glycopeptide identification	116:158	A key point in achieving accurate intact glycopeptide identification is the definition of the glycan composition file that is used to match experimental with theoretical masses by a glycoproteomics search engine.					
32636234	3	53	theme	composition	502:512	arg1	file					514:517	a default composition file	492:517	a default composition file that is readily used when processing MS data	492:562	Most glycoproteomics search engines include a default composition file that is readily used when processing MS data.					
32636234	0	54	theme	GlyConnect	68:77	arg1	Compozitor					79:88	GlyConnect Compozitor	68:88	GlyConnect Compozitor	68:88	Examining and Fine-tuning the Selection of Glycan Compositions with GlyConnect Compozitor.					
32636234	8	55	theme	GlyConnect	1281:1290	arg1	database					1292:1299	the GlyConnect database	1277:1299	the GlyConnect database	1277:1299	They also confirm the consistency of a set of compositions based on the content of the GlyConnect database.					
32636234	3	56	theme	search	469:474	arg1	engines					476:482	Most glycoproteomics search engines	448:482	Most glycoproteomics search engines	448:482	Most glycoproteomics search engines include a default composition file that is readily used when processing MS data.					
32636234	3	57	theme	processing	545:554	arg1	data					559:562	processing MS data	545:562	processing MS data	545:562	Most glycoproteomics search engines include a default composition file that is readily used when processing MS data.					
33087860	3	0	theme	Host-derived	345:356	arg1	glycans					364:370	Host-derived mucus glycans	345:370	Host-derived mucus glycans on gut-secreted mucin proteins	345:401	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	0	theme	Host-derived	345:356	arg1	source					436:441	a continuous endogenous source	412:441	a continuous endogenous source of MACs for resident microbes	412:471	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	1	1	theme	function	185:192	arg1	carbohydrates					110:122	Microbiota-accessible carbohydrates	88:122	Microbiota-accessible carbohydrates (MACs)	88:129	Microbiota-accessible carbohydrates (MACs) are powerful modulators of microbiota composition and function.					
33087860	1	1	theme	function	185:192	arg1	modulators					144:153	powerful modulators	135:153	powerful modulators of microbiota composition and function	135:192	Microbiota-accessible carbohydrates (MACs) are powerful modulators of microbiota composition and function.					
33087860	4	2	from	shaping	722:728	arg1	model					780:784	a mouse model	772:784	a mouse model	772:784	In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model.					
33087860	3	3	theme	mucus	358:362	arg1	glycans					364:370	Host-derived mucus glycans	345:370	Host-derived mucus glycans on gut-secreted mucin proteins	345:401	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	3	theme	mucus	358:362	arg1	source					436:441	a continuous endogenous source	412:441	a continuous endogenous source of MACs for resident microbes	412:471	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	7	4	from	rare	1261:1264	arg1	abundant					1208:1215	abundant	1208:1215	abundant	1208:1215	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	7	4	from	rare	1261:1264	arg1	pathogens					1277:1285	enteric pathogens	1269:1285	enteric pathogens	1269:1285	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	6	5	theme	Akkermansia	1106:1116	arg1	muciniphila					1118:1128	resident Akkermansia muciniphila	1097:1128	resident Akkermansia muciniphila	1097:1128	We demonstrate that PMGs aid recovery of the microbiota after antibiotic treatment, suppress Clostridium difficile abundance, delay the onset of diet-induced obesity, and increase the relative abundance of resident Akkermansia muciniphila.					
33087860	6	6	theme	muciniphila	1118:1128	arg1	abundance					1084:1092	the relative abundance	1071:1092	the relative abundance of resident Akkermansia muciniphila	1071:1128	We demonstrate that PMGs aid recovery of the microbiota after antibiotic treatment, suppress Clostridium difficile abundance, delay the onset of diet-induced obesity, and increase the relative abundance of resident Akkermansia muciniphila.					
33087860	3	7	theme	endogenous	425:434	arg1	glycans					364:370	Host-derived mucus glycans	345:370	Host-derived mucus glycans on gut-secreted mucin proteins	345:401	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	7	theme	endogenous	425:434	arg1	source					436:441	a continuous endogenous source	412:441	a continuous endogenous source of MACs for resident microbes	412:471	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	7	8	theme	gut	1242:1244	arg1	commensals					1246:1255	prevalent gut commensals	1232:1255	prevalent gut commensals	1232:1255	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	3	9	theme	healthy	577:583	arg1	community					595:603	a healthy microbial community	575:603	a healthy microbial community	575:603	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	10	theme	gut-secreted	375:386	arg1	proteins					394:401	gut-secreted mucin proteins	375:401	gut-secreted mucin proteins	375:401	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	11	theme	purified	516:523	arg1	glycans					552:558	purified, orally administered mucus glycans	516:558	purified, orally administered mucus glycans	516:558	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	0	12	link	Mucin-derived	0:12	arg1	O-glycans					14:22	Mucin-derived O-glycans	0:22	Mucin-derived O-glycans supplemented to diet	0:43	Mucin-derived O-glycans supplemented to diet mitigate diverse microbiota perturbations.					
33087860	1	13	theme	Microbiota-accessible	88:108	arg1	MACs					125:128	MACs	125:128	MACs	125:128	Microbiota-accessible carbohydrates (MACs) are powerful modulators of microbiota composition and function.					
33087860	1	13	theme	Microbiota-accessible	88:108	arg1	carbohydrates					110:122	Microbiota-accessible carbohydrates	88:122	Microbiota-accessible carbohydrates (MACs)	88:129	Microbiota-accessible carbohydrates (MACs) are powerful modulators of microbiota composition and function.					
33087860	1	13	theme	Microbiota-accessible	88:108	arg1	modulators					144:153	powerful modulators	135:153	powerful modulators of microbiota composition and function	135:192	Microbiota-accessible carbohydrates (MACs) are powerful modulators of microbiota composition and function.					
33087860	2	14	attach	derived	222:228	arg1	diet					235:238	diet	235:238	diet	235:238	These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding.					
33087860	2	14	attach	derived	222:228	arg2	substrates					201:210	These substrates	195:210	These substrates	195:210	These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding.					
33087860	5	15	theme	similar	864:870	arg1	microbes					881:888	taxonomically similar resident microbes	850:888	taxonomically similar resident microbes	850:888	We found that porcine mucin glycans (PMGs) and HMOs enrich for taxonomically similar resident microbes.					
33087860	3	16	theme	mucin	388:392	arg1	proteins					394:401	gut-secreted mucin proteins	375:401	gut-secreted mucin proteins	375:401	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	7	17	from	abundant	1208:1215	arg1	rare					1261:1264	rare	1261:1264	rare in enteric pathogens	1261:1285	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	7	17	from	abundant	1208:1215	arg1	commensals					1246:1255	prevalent gut commensals	1232:1255	prevalent gut commensals	1232:1255	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	7	18	theme	In	1131:1132	arg1	analysis					1141:1148	In silico analysis	1131:1148	In silico analysis	1131:1148	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	7	19	theme	prevalent	1232:1240	arg1	commensals					1246:1255	prevalent gut commensals	1232:1255	prevalent gut commensals	1232:1255	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	2	20	theme	milk	294:297	arg1	HMOs					317:320	HMOs	317:320	HMOs	317:320	These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding.					
33087860	2	20	theme	milk	294:297	arg1	oligosaccharides					299:314	human milk oligosaccharides	288:314	human milk oligosaccharides (HMOs)	288:321	These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding.					
33087860	5	21	theme	resident	872:879	arg1	microbes					881:888	taxonomically similar resident microbes	850:888	taxonomically similar resident microbes	850:888	We found that porcine mucin glycans (PMGs) and HMOs enrich for taxonomically similar resident microbes.					
33087860	3	22	theme	continuous	414:423	arg1	glycans					364:370	Host-derived mucus glycans	345:370	Host-derived mucus glycans on gut-secreted mucin proteins	345:401	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	22	theme	continuous	414:423	arg1	source					436:441	a continuous endogenous source	412:441	a continuous endogenous source of MACs for resident microbes	412:471	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	6	23	theme	resident	1097:1104	arg1	muciniphila					1118:1128	resident Akkermansia muciniphila	1097:1128	resident Akkermansia muciniphila	1097:1128	We demonstrate that PMGs aid recovery of the microbiota after antibiotic treatment, suppress Clostridium difficile abundance, delay the onset of diet-induced obesity, and increase the relative abundance of resident Akkermansia muciniphila.					
33087860	3	24	link	Host-derived	345:356	arg1	glycans					364:370	Host-derived mucus glycans	345:370	Host-derived mucus glycans on gut-secreted mucin proteins	345:401	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	24	link	Host-derived	345:356	arg1	source					436:441	a continuous endogenous source	412:441	a continuous endogenous source of MACs for resident microbes	412:471	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	25	theme	mucus	546:550	arg1	glycans					552:558	purified, orally administered mucus glycans	516:558	purified, orally administered mucus glycans	516:558	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	26	theme	resident	455:462	arg1	microbes					464:471	resident microbes	455:471	resident microbes	455:471	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	0	27	theme	Mucin-derived	0:12	arg1	O-glycans					14:22	Mucin-derived O-glycans	0:22	Mucin-derived O-glycans supplemented to diet	0:43	Mucin-derived O-glycans supplemented to diet mitigate diverse microbiota perturbations.					
33087860	6	28	theme	difficile	996:1004	arg1	abundance					1006:1014	Clostridium difficile abundance	984:1014	Clostridium difficile abundance	984:1014	We demonstrate that PMGs aid recovery of the microbiota after antibiotic treatment, suppress Clostridium difficile abundance, delay the onset of diet-induced obesity, and increase the relative abundance of resident Akkermansia muciniphila.					
33087860	4	29	theme	microbiota	758:767	arg1	recovery					734:741	the recovery	730:741	the recovery of a perturbed microbiota	730:767	In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model.					
33087860	2	30	from	plants	278:283	arg1	HMOs					317:320	HMOs	317:320	HMOs	317:320	These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding.					
33087860	2	30	from	plants	278:283	arg1	polysaccharides					257:271	complex polysaccharides	249:271	complex polysaccharides from plants	249:283	These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding.					
33087860	2	30	from	plants	278:283	arg1	oligosaccharides					299:314	human milk oligosaccharides	288:314	human milk oligosaccharides (HMOs)	288:321	These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding.					
33087860	6	31	theme	relative	1075:1082	arg1	abundance					1084:1092	the relative abundance	1071:1092	the relative abundance of resident Akkermansia muciniphila	1071:1128	We demonstrate that PMGs aid recovery of the microbiota after antibiotic treatment, suppress Clostridium difficile abundance, delay the onset of diet-induced obesity, and increase the relative abundance of resident Akkermansia muciniphila.					
33087860	6	32	theme	Clostridium	984:994	arg1	difficile					996:1004	Clostridium difficile	984:1004	Clostridium difficile abundance	984:1014	We demonstrate that PMGs aid recovery of the microbiota after antibiotic treatment, suppress Clostridium difficile abundance, delay the onset of diet-induced obesity, and increase the relative abundance of resident Akkermansia muciniphila.					
33087860	3	33	theme	microbial	585:593	arg1	community					595:603	a healthy microbial community	575:603	a healthy microbial community	575:603	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	34	dep	purified	516:523	arg1	administered					533:544	administered	533:544	administered	533:544	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	4	35	link	O-linked	647:654	arg1	glycans					656:662	O-linked glycans	647:662	O-linked glycans	647:662	In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model.					
33087860	7	36	theme	host	1366:1369	arg1	development					1371:1381	host development	1366:1381	host development	1366:1381	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	3	37	theme	potential	498:506	arg1	role					508:511	the potential role	494:511	the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community	494:603	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	38	theme	MACs	446:449	arg1	glycans					364:370	Host-derived mucus glycans	345:370	Host-derived mucus glycans on gut-secreted mucin proteins	345:401	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	3	38	theme	MACs	446:449	arg1	source					436:441	a continuous endogenous source	412:441	a continuous endogenous source of MACs for resident microbes	412:471	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	8	39	theme	host	1610:1613	arg1	physiology					1615:1624	host physiology	1610:1624	host physiology	1610:1624	Importantly, we identify mucus glycans as a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology.					
33087860	4	40	theme	gastric	677:683	arg1	mucin					685:689	porcine gastric mucin	669:689	porcine gastric mucin	669:689	In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model.					
33087860	5	41	theme	porcine	801:807	arg1	PMGs					824:827	PMGs	824:827	PMGs	824:827	We found that porcine mucin glycans (PMGs) and HMOs enrich for taxonomically similar resident microbes.					
33087860	5	41	theme	porcine	801:807	arg1	glycans					815:821	porcine mucin glycans	801:821	porcine mucin glycans (PMGs)	801:828	We found that porcine mucin glycans (PMGs) and HMOs enrich for taxonomically similar resident microbes.					
33087860	7	42	theme	mucus	1186:1190	arg1	utilization					1192:1202	mucus utilization	1186:1202	mucus utilization	1186:1202	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	8	43	theme	compounds	1515:1523	arg1	class					1496:1500	a novel class	1488:1500	a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology	1488:1624	Importantly, we identify mucus glycans as a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology.					
33087860	8	43	theme	compounds	1515:1523	arg1	glycans					1477:1483	mucus glycans	1471:1483	mucus glycans	1471:1483	Importantly, we identify mucus glycans as a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology.					
33087860	2	44	theme	complex	249:255	arg1	polysaccharides					257:271	complex polysaccharides	249:271	complex polysaccharides from plants	249:283	These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding.					
33087860	4	45	theme	porcine	669:675	arg1	mucin					685:689	porcine gastric mucin	669:689	porcine gastric mucin	669:689	In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model.					
33087860	0	46	theme	microbiota	62:71	arg1	perturbations					73:85	diverse microbiota perturbations	54:85	diverse microbiota perturbations	54:85	Mucin-derived O-glycans supplemented to diet mitigate diverse microbiota perturbations.					
33087860	6	47	theme	antibiotic	953:962	arg1	treatment					964:972	antibiotic treatment	953:972	antibiotic treatment	953:972	We demonstrate that PMGs aid recovery of the microbiota after antibiotic treatment, suppress Clostridium difficile abundance, delay the onset of diet-induced obesity, and increase the relative abundance of resident Akkermansia muciniphila.					
33087860	8	48	theme	prebiotic	1505:1513	arg1	compounds					1515:1523	prebiotic compounds	1505:1523	prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology	1505:1624	Importantly, we identify mucus glycans as a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology.					
33087860	7	49	theme	host-microbe	1419:1430	arg1	relationship					1432:1443	the host-microbe relationship	1415:1443	the host-microbe relationship	1415:1443	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	1	50	theme	powerful	135:142	arg1	carbohydrates					110:122	Microbiota-accessible carbohydrates	88:122	Microbiota-accessible carbohydrates (MACs)	88:129	Microbiota-accessible carbohydrates (MACs) are powerful modulators of microbiota composition and function.					
33087860	1	50	theme	powerful	135:142	arg1	modulators					144:153	powerful modulators	135:153	powerful modulators of microbiota composition and function	135:192	Microbiota-accessible carbohydrates (MACs) are powerful modulators of microbiota composition and function.					
33087860	0	51	theme	diverse	54:60	arg1	perturbations					73:85	diverse microbiota perturbations	54:85	diverse microbiota perturbations	54:85	Mucin-derived O-glycans supplemented to diet mitigate diverse microbiota perturbations.					
33087860	8	52	theme	mucus	1471:1475	arg1	class					1496:1500	a novel class	1488:1500	a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology	1488:1624	Importantly, we identify mucus glycans as a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology.					
33087860	8	52	theme	mucus	1471:1475	arg1	glycans					1477:1483	mucus glycans	1471:1483	mucus glycans	1471:1483	Importantly, we identify mucus glycans as a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology.					
33087860	7	53	from	commensals	1246:1255	arg1	abundant					1208:1215	abundant	1208:1215	abundant	1208:1215	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	7	53	from	commensals	1246:1255	arg1	pathogens					1277:1285	enteric pathogens	1269:1285	enteric pathogens	1269:1285	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	7	54	theme	relationship	1432:1443	arg1	evolution					1402:1410	the evolution	1398:1410	the evolution of the host-microbe relationship	1398:1443	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	7	55	theme	glycan-degrading	1310:1325	arg1	capabilities					1327:1338	these glycan-degrading capabilities	1304:1338	these glycan-degrading capabilities	1304:1338	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	6	56	theme	microbiota	936:945	arg1	recovery					920:927	recovery	920:927	recovery of the microbiota after antibiotic treatment	920:972	We demonstrate that PMGs aid recovery of the microbiota after antibiotic treatment, suppress Clostridium difficile abundance, delay the onset of diet-induced obesity, and increase the relative abundance of resident Akkermansia muciniphila.					
33087860	2	57	theme	human	288:292	arg1	HMOs					317:320	HMOs	317:320	HMOs	317:320	These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding.					
33087860	2	57	theme	human	288:292	arg1	oligosaccharides					299:314	human milk oligosaccharides	288:314	human milk oligosaccharides (HMOs)	288:321	These substrates are often derived from diet, such as complex polysaccharides from plants or human milk oligosaccharides (HMOs) during breastfeeding.					
33087860	4	58	theme	perturbed	748:756	arg1	microbiota					758:767	a perturbed microbiota	746:767	a perturbed microbiota	746:767	In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model.					
33087860	4	59	theme	mouse	774:778	arg1	model					780:784	a mouse model	772:784	a mouse model	772:784	In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model.					
33087860	6	60	theme	obesity	1049:1055	arg1	onset					1027:1031	the onset	1023:1031	the onset of diet-induced obesity	1023:1055	We demonstrate that PMGs aid recovery of the microbiota after antibiotic treatment, suppress Clostridium difficile abundance, delay the onset of diet-induced obesity, and increase the relative abundance of resident Akkermansia muciniphila.					
33087860	3	61	from	glycans	364:370	arg1	proteins					394:401	gut-secreted mucin proteins	375:401	gut-secreted mucin proteins	375:401	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	1	62	theme	microbiota	158:167	arg1	composition					169:179	microbiota composition	158:179	microbiota composition	158:179	Microbiota-accessible carbohydrates (MACs) are powerful modulators of microbiota composition and function.					
33087860	6	63	theme	diet-induced	1036:1047	arg1	obesity					1049:1055	diet-induced obesity	1036:1055	diet-induced obesity	1036:1055	We demonstrate that PMGs aid recovery of the microbiota after antibiotic treatment, suppress Clostridium difficile abundance, delay the onset of diet-induced obesity, and increase the relative abundance of resident Akkermansia muciniphila.					
33087860	4	64	dep	liberated	624:632	arg1	purified					638:645	purified	638:645	purified O-linked glycans from porcine gastric mucin	638:689	In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model.					
33087860	4	64	dep	liberated	624:632	arg1	assessed					695:702	assessed	695:702	assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model	695:784	In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model.					
33087860	8	65	theme	novel	1490:1494	arg1	class					1496:1500	a novel class	1488:1500	a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology	1488:1624	Importantly, we identify mucus glycans as a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology.					
33087860	8	65	theme	novel	1490:1494	arg1	glycans					1477:1483	mucus glycans	1471:1483	mucus glycans	1471:1483	Importantly, we identify mucus glycans as a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology.					
33087860	1	66	theme	composition	169:179	arg1	carbohydrates					110:122	Microbiota-accessible carbohydrates	88:122	Microbiota-accessible carbohydrates (MACs)	88:129	Microbiota-accessible carbohydrates (MACs) are powerful modulators of microbiota composition and function.					
33087860	1	66	theme	composition	169:179	arg1	modulators					144:153	powerful modulators	135:153	powerful modulators of microbiota composition and function	135:192	Microbiota-accessible carbohydrates (MACs) are powerful modulators of microbiota composition and function.					
33087860	7	67	dep	In	1131:1132	arg1	silico					1134:1139	silico	1134:1139	silico	1134:1139	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	4	68	theme	O-linked	647:654	arg1	glycans					656:662	O-linked glycans	647:662	O-linked glycans	647:662	In this study, we liberated and purified O-linked glycans from porcine gastric mucin and assessed their efficacy in shaping the recovery of a perturbed microbiota in a mouse model.					
33087860	5	69	theme	mucin	809:813	arg1	PMGs					824:827	PMGs	824:827	PMGs	824:827	We found that porcine mucin glycans (PMGs) and HMOs enrich for taxonomically similar resident microbes.					
33087860	5	69	theme	mucin	809:813	arg1	glycans					815:821	porcine mucin glycans	801:821	porcine mucin glycans (PMGs)	801:828	We found that porcine mucin glycans (PMGs) and HMOs enrich for taxonomically similar resident microbes.					
33087860	3	70	theme	glycans	552:558	arg1	role					508:511	the potential role	494:511	the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community	494:603	Host-derived mucus glycans on gut-secreted mucin proteins serve as a continuous endogenous source of MACs for resident microbes; here we investigate the potential role of purified, orally administered mucus glycans in maintaining a healthy microbial community.					
33087860	7	71	theme	enteric	1269:1275	arg1	pathogens					1277:1285	enteric pathogens	1269:1285	enteric pathogens	1269:1285	In silico analysis revealed that genes associated with mucus utilization are abundant and diverse in prevalent gut commensals and rare in enteric pathogens, consistent with these glycan-degrading capabilities being selected for during host development and throughout the evolution of the host-microbe relationship.					
33087860	8	72	used	used	1537:1540	arg2	compounds					1515:1523	prebiotic compounds	1505:1523	prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology	1505:1624	Importantly, we identify mucus glycans as a novel class of prebiotic compounds that can be used to mitigate perturbations to the microbiota and provide benefits to host physiology.					
32998456	0	0	theme	MS	71:72	arg1	Spectra					74:80	MALDI-TOF MS Spectra	61:80	MALDI-TOF MS Spectra	61:80	Toolbox Accelerating Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra and Mapping Expression Variation to Biosynthetic Pathways.					
32998456	6	1	theme	TAG	988:990	arg1	process					992:998	the TAG process	984:998	the TAG process	984:998	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	3	2	theme	glycomic	538:545	arg1	analysis					547:554	glycomic analysis	538:554	glycomic analysis	538:554	These differences pose a challenge to developing useful software for glycomic analysis.					
32998456	4	3	theme	Accelerating	614:625	arg1	program					643:649	the novel Toolbox Accelerating Glycomics (TAG) program	596:649	the novel Toolbox Accelerating Glycomics (TAG) program	596:649	To overcome this problem, we developed the novel Toolbox Accelerating Glycomics (TAG) program.					
32998456	0	4	theme	MALDI-TOF	61:69	arg1	Spectra					74:80	MALDI-TOF MS Spectra	61:80	MALDI-TOF MS Spectra	61:80	Toolbox Accelerating Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra and Mapping Expression Variation to Biosynthetic Pathways.					
32998456	8	5	theme	glycomic	1435:1442	arg1	platform					1444:1451	the glycomic platform	1431:1451	the glycomic platform	1431:1451	Although this study was limited to the analysis of N-glycans and free oligosaccharides, the glycomic platform will be expanded to facilitate the analysis of O-glycans and glycans of glycosphingolipids.					
32998456	1	6	theme	extraordinary	157:169	arg1	diversity					182:190	extraordinary structural diversity	157:190	extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis	157:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	4	7	theme	Toolbox	606:612	arg1	program					643:649	the novel Toolbox Accelerating Glycomics (TAG) program	596:649	the novel Toolbox Accelerating Glycomics (TAG) program	596:649	To overcome this problem, we developed the novel Toolbox Accelerating Glycomics (TAG) program.					
32998456	2	8	contain	have	374:377	arg1	glycans					366:372	glycans	366:372	glycans	366:372	Unlike linear DNA and protein sequences, glycans have heterogeneous structures that differ in composition, branching, linkage, and anomericity.					
32998456	2	8	contain	have	374:377	arg2	structures					393:402	heterogeneous structures	379:402	heterogeneous structures that differ in composition, branching, linkage, and anomericity	379:466	Unlike linear DNA and protein sequences, glycans have heterogeneous structures that differ in composition, branching, linkage, and anomericity.					
32998456	2	9	theme	heterogeneous	379:391	arg1	structures					393:402	heterogeneous structures	379:402	heterogeneous structures that differ in composition, branching, linkage, and anomericity	379:466	Unlike linear DNA and protein sequences, glycans have heterogeneous structures that differ in composition, branching, linkage, and anomericity.					
32998456	7	10	theme	manual	1326:1331	arg1	analysis					1333:1340	manual analysis	1326:1340	manual analysis	1326:1340	TAG not only drastically reduced the amount of time and labor needed for glycomic analysis but also detected and quantified more glycans than manual analysis.					
32998456	5	11	used	used	722:725	arg2	list					709:712	a glycan list	700:712	a glycan list that is used for database searching in TAG Expression	700:766	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	6	12	theme	knockout	1158:1165	arg1	gene					1147:1150	a functional Npc1 gene	1129:1150	a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1129:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	6	12	theme	knockout	1158:1165	arg1	cells					1176:1180	Npc1 knockout (KO) CHO cells	1153:1180	Npc1 knockout (KO) CHO cells	1153:1180	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	6	13	theme	Npc1	1142:1145	arg1	cells					1176:1180	Npc1 knockout (KO) CHO cells	1153:1180	Npc1 knockout (KO) CHO cells	1153:1180	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	6	13	theme	Npc1	1142:1145	arg1	gene					1147:1150	a functional Npc1 gene	1129:1150	a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1129:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	4	14	theme	Glycomics	627:635	arg1	program					643:649	the novel Toolbox Accelerating Glycomics (TAG) program	596:649	the novel Toolbox Accelerating Glycomics (TAG) program	596:649	To overcome this problem, we developed the novel Toolbox Accelerating Glycomics (TAG) program.					
32998456	0	15	theme	Expression	94:103	arg1	Variation					105:113	Mapping Expression Variation	86:113	Mapping Expression Variation to Biosynthetic Pathways	86:138	Toolbox Accelerating Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra and Mapping Expression Variation to Biosynthetic Pathways.					
32998456	6	16	theme	profile	1057:1063	arg1	data					1065:1068	glycomic expression profile data	1037:1068	glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1037:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	6	17	theme	functional	1131:1140	arg1	cells					1176:1180	Npc1 knockout (KO) CHO cells	1153:1180	Npc1 knockout (KO) CHO cells	1153:1180	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	6	17	theme	functional	1131:1140	arg1	gene					1147:1150	a functional Npc1 gene	1129:1150	a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1129:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	6	18	theme	glycomic	1037:1044	arg1	data					1065:1068	glycomic expression profile data	1037:1068	glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1037:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	1	19	theme	structural	171:180	arg1	diversity					182:190	extraordinary structural diversity	157:190	extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis	157:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	0	20	theme	Mapping	86:92	arg1	Variation					105:113	Mapping Expression Variation	86:113	Mapping Expression Variation to Biosynthetic Pathways	86:138	Toolbox Accelerating Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra and Mapping Expression Variation to Biosynthetic Pathways.					
32998456	4	21	theme	TAG	638:640	arg1	program					643:649	the novel Toolbox Accelerating Glycomics (TAG) program	596:649	the novel Toolbox Accelerating Glycomics (TAG) program	596:649	To overcome this problem, we developed the novel Toolbox Accelerating Glycomics (TAG) program.					
32998456	5	22	theme	database	731:738	arg1	searching					740:748	database searching	731:748	database searching in TAG Expression	731:766	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	2	23	theme	protein	347:353	arg1	sequences					355:363	protein sequences	347:363	protein sequences	347:363	Unlike linear DNA and protein sequences, glycans have heterogeneous structures that differ in composition, branching, linkage, and anomericity.					
32998456	1	24	theme	commensurate	192:203	arg1	diversity					182:190	extraordinary structural diversity	157:190	extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis	157:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	5	25	theme	biosynthetic	920:931	arg1	pathways					933:940	biosynthetic pathways	920:940	biosynthetic pathways	920:940	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	5	26	dep	units	674:678	arg1	creates					692:698	creates	692:698	creates a glycan list that is used for database searching in TAG Expression	692:766	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	5	26	dep	units	674:678	arg1	annotates					800:808	annotates	800:808	annotates	800:808	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	5	26	dep	units	674:678	arg1	draws					844:848	draws	844:848	draws graphs	844:855	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	5	26	dep	units	674:678	arg1	quantifies					814:823	quantifies	814:823	quantifies glycan signals	814:838	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	5	27	theme	glycan	702:707	arg1	list					709:712	a glycan list	700:712	a glycan list that is used for database searching in TAG Expression	700:766	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	6	28	from	cells	1103:1107	arg1	data					1065:1068	glycomic expression profile data	1037:1068	glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1037:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	5	29	theme	glycan	825:830	arg1	signals					832:838	glycan signals	825:838	glycan signals	825:838	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	0	30	theme	Glycomics	21:29	arg1	Annotation					45:54	Glycomics (TAG): Glycan Annotation	21:54	Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra	21:80	Toolbox Accelerating Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra and Mapping Expression Variation to Biosynthetic Pathways.					
32998456	4	31	theme	novel	600:604	arg1	program					643:649	the novel Toolbox Accelerating Glycomics (TAG) program	596:649	the novel Toolbox Accelerating Glycomics (TAG) program	596:649	To overcome this problem, we developed the novel Toolbox Accelerating Glycomics (TAG) program.					
32998456	2	32	theme	linear	332:337	arg1	DNA					339:341	linear DNA	332:341	linear DNA	332:341	Unlike linear DNA and protein sequences, glycans have heterogeneous structures that differ in composition, branching, linkage, and anomericity.					
32998456	6	33	theme	hamster	1083:1089	arg1	cells					1103:1107	Chinese hamster ovary (CHO) cells	1075:1107	Chinese hamster ovary (CHO) cells	1075:1107	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	6	34	theme	CHO	1098:1100	arg1	cells					1103:1107	Chinese hamster ovary (CHO) cells	1075:1107	Chinese hamster ovary (CHO) cells	1075:1107	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	5	35	from	searching	740:748	arg1	Expression					757:766	TAG Expression	753:766	TAG Expression	753:766	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	7	36	theme	more	1308:1311	arg1	glycans					1313:1319	more glycans	1308:1319	more glycans	1308:1319	TAG not only drastically reduced the amount of time and labor needed for glycomic analysis but also detected and quantified more glycans than manual analysis.					
32998456	6	37	from	mutants	1113:1119	arg1	data					1065:1068	glycomic expression profile data	1037:1068	glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1037:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	0	38	theme	TAG	32:34	arg1	Annotation					45:54	Glycomics (TAG): Glycan Annotation	21:54	Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra	21:80	Toolbox Accelerating Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra and Mapping Expression Variation to Biosynthetic Pathways.					
32998456	8	39	theme	O-glycans	1500:1508	arg1	analysis					1488:1495	the analysis	1484:1495	the analysis of O-glycans and glycans of glycosphingolipids	1484:1542	Although this study was limited to the analysis of N-glycans and free oligosaccharides, the glycomic platform will be expanded to facilitate the analysis of O-glycans and glycans of glycosphingolipids.					
32998456	0	40	theme	Biosynthetic	118:129	arg1	Pathways					131:138	Biosynthetic Pathways	118:138	Biosynthetic Pathways	118:138	Toolbox Accelerating Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra and Mapping Expression Variation to Biosynthetic Pathways.					
32998456	6	41	theme	CHO	1172:1174	arg1	gene					1147:1150	a functional Npc1 gene	1129:1150	a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1129:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	6	41	theme	CHO	1172:1174	arg1	cells					1176:1180	Npc1 knockout (KO) CHO cells	1153:1180	Npc1 knockout (KO) CHO cells	1153:1180	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	1	42	theme	numerous	231:238	arg1	processes					261:269	numerous fundamental cellular processes	231:269	numerous fundamental cellular processes including growth, differentiation, and morphogenesis	231:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	42	theme	numerous	231:238	arg1	differentiation					289:303	differentiation	289:303	differentiation	289:303	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	42	theme	numerous	231:238	arg1	morphogenesis					310:322	morphogenesis	310:322	morphogenesis	310:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	42	theme	numerous	231:238	arg1	growth					281:286	growth	281:286	growth	281:286	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	0	43	theme	Glycan	38:43	arg1	Annotation					45:54	Glycomics (TAG): Glycan Annotation	21:54	Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra	21:80	Toolbox Accelerating Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra and Mapping Expression Variation to Biosynthetic Pathways.					
32998456	1	44	with	commensurate	192:203	arg1	involvement					216:226	their involvement	210:226	their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis	210:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	8	45	theme	oligosaccharides	1413:1428	arg1	analysis					1382:1389	the analysis	1378:1389	the analysis of N-glycans and free oligosaccharides	1378:1428	Although this study was limited to the analysis of N-glycans and free oligosaccharides, the glycomic platform will be expanded to facilitate the analysis of O-glycans and glycans of glycosphingolipids.					
32998456	8	46	theme	glycans	1514:1520	arg1	analysis					1488:1495	the analysis	1484:1495	the analysis of O-glycans and glycans of glycosphingolipids	1484:1542	Although this study was limited to the analysis of N-glycans and free oligosaccharides, the glycomic platform will be expanded to facilitate the analysis of O-glycans and glycans of glycosphingolipids.					
32998456	8	47	theme	N-glycans	1394:1402	arg1	analysis					1382:1389	the analysis	1378:1389	the analysis of N-glycans and free oligosaccharides	1378:1428	Although this study was limited to the analysis of N-glycans and free oligosaccharides, the glycomic platform will be expanded to facilitate the analysis of O-glycans and glycans of glycosphingolipids.					
32998456	6	48	theme	KO	1168:1169	arg1	gene					1147:1150	a functional Npc1 gene	1129:1150	a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1129:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	6	48	theme	KO	1168:1169	arg1	cells					1176:1180	Npc1 knockout (KO) CHO cells	1153:1180	Npc1 knockout (KO) CHO cells	1153:1180	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	7	49	theme	glycomic	1257:1264	arg1	analysis					1266:1273	glycomic analysis	1257:1273	glycomic analysis	1257:1273	TAG not only drastically reduced the amount of time and labor needed for glycomic analysis but also detected and quantified more glycans than manual analysis.					
32998456	5	50	theme	obtained	885:892	arg1	information					905:915	the obtained expression information	881:915	the obtained expression information to biosynthetic pathways	881:940	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	1	51	from	involvement	216:226	arg1	processes					261:269	numerous fundamental cellular processes	231:269	numerous fundamental cellular processes including growth, differentiation, and morphogenesis	231:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	51	from	involvement	216:226	arg1	differentiation					289:303	differentiation	289:303	differentiation	289:303	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	51	from	involvement	216:226	arg1	morphogenesis					310:322	morphogenesis	310:322	morphogenesis	310:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	51	from	involvement	216:226	arg1	growth					281:286	growth	281:286	growth	281:286	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	5	52	theme	expression	894:903	arg1	information					905:915	the obtained expression information	881:915	the obtained expression information to biosynthetic pathways	881:940	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	6	53	theme	ovary	1091:1095	arg1	cells					1103:1107	Chinese hamster ovary (CHO) cells	1075:1107	Chinese hamster ovary (CHO) cells	1075:1107	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	1	54	theme	fundamental	240:250	arg1	processes					261:269	numerous fundamental cellular processes	231:269	numerous fundamental cellular processes including growth, differentiation, and morphogenesis	231:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	54	theme	fundamental	240:250	arg1	differentiation					289:303	differentiation	289:303	differentiation	289:303	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	54	theme	fundamental	240:250	arg1	morphogenesis					310:322	morphogenesis	310:322	morphogenesis	310:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	54	theme	fundamental	240:250	arg1	growth					281:286	growth	281:286	growth	281:286	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	8	55	theme	free	1408:1411	arg1	oligosaccharides					1413:1428	free oligosaccharides	1408:1428	free oligosaccharides	1408:1428	Although this study was limited to the analysis of N-glycans and free oligosaccharides, the glycomic platform will be expanded to facilitate the analysis of O-glycans and glycans of glycosphingolipids.					
32998456	5	56	dep	draws	844:848	arg1	graphs					850:855	graphs	850:855	draws graphs	844:855	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	3	57	theme	useful	518:523	arg1	software					525:532	useful software	518:532	useful software	518:532	These differences pose a challenge to developing useful software for glycomic analysis.					
32998456	7	58	theme	labor	1240:1244	arg1	time					1231:1234	time	1231:1234	time	1231:1234	TAG not only drastically reduced the amount of time and labor needed for glycomic analysis but also detected and quantified more glycans than manual analysis.					
32998456	7	58	theme	labor	1240:1244	arg1	amount					1221:1226	the amount	1217:1226	the amount of time and labor needed for glycomic analysis	1217:1273	TAG not only drastically reduced the amount of time and labor needed for glycomic analysis but also detected and quantified more glycans than manual analysis.					
32998456	7	58	theme	labor	1240:1244	arg1	labor					1240:1244	labor	1240:1244	labor	1240:1244	TAG not only drastically reduced the amount of time and labor needed for glycomic analysis but also detected and quantified more glycans than manual analysis.					
32998456	1	59	theme	cellular	252:259	arg1	processes					261:269	numerous fundamental cellular processes	231:269	numerous fundamental cellular processes including growth, differentiation, and morphogenesis	231:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	59	theme	cellular	252:259	arg1	differentiation					289:303	differentiation	289:303	differentiation	289:303	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	59	theme	cellular	252:259	arg1	morphogenesis					310:322	morphogenesis	310:322	morphogenesis	310:322	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	1	59	theme	cellular	252:259	arg1	growth					281:286	growth	281:286	growth	281:286	Glycans present extraordinary structural diversity commensurate with their involvement in numerous fundamental cellular processes including growth, differentiation, and morphogenesis.					
32998456	0	60	from	Spectra	74:80	arg1	Annotation					45:54	Glycomics (TAG): Glycan Annotation	21:54	Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra	21:80	Toolbox Accelerating Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra and Mapping Expression Variation to Biosynthetic Pathways.					
32998456	0	60	from	Spectra	74:80	arg1	Variation					105:113	Mapping Expression Variation	86:113	Mapping Expression Variation to Biosynthetic Pathways	86:138	Toolbox Accelerating Glycomics (TAG): Glycan Annotation from MALDI-TOF MS Spectra and Mapping Expression Variation to Biosynthetic Pathways.					
32998456	6	61	theme	Npc1	1153:1156	arg1	gene					1147:1150	a functional Npc1 gene	1129:1150	a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1129:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	6	61	theme	Npc1	1153:1156	arg1	cells					1176:1180	Npc1 knockout (KO) CHO cells	1153:1180	Npc1 knockout (KO) CHO cells	1153:1180	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	7	62	theme	time	1231:1234	arg1	time					1231:1234	time	1231:1234	time	1231:1234	TAG not only drastically reduced the amount of time and labor needed for glycomic analysis but also detected and quantified more glycans than manual analysis.					
32998456	7	62	theme	time	1231:1234	arg1	amount					1221:1226	the amount	1217:1226	the amount of time and labor needed for glycomic analysis	1217:1273	TAG not only drastically reduced the amount of time and labor needed for glycomic analysis but also detected and quantified more glycans than manual analysis.					
32998456	7	62	theme	time	1231:1234	arg1	labor					1240:1244	labor	1240:1244	labor	1240:1244	TAG not only drastically reduced the amount of time and labor needed for glycomic analysis but also detected and quantified more glycans than manual analysis.					
32998456	5	63	theme	TAG	863:865	arg1	Pathway					867:873	'TAG Pathway'	862:874	'TAG Pathway'	862:874	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	6	64	theme	expression	1046:1055	arg1	data					1065:1068	glycomic expression profile data	1037:1068	glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells)	1037:1181	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32998456	5	65	theme	TAG	682:684	arg1	List					686:689	'TAG List'	681:690	'TAG List'	681:690	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	5	66	theme	TAG	753:755	arg1	Expression					757:766	TAG Expression	753:766	TAG Expression	753:766	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	8	67	theme	glycosphingolipids	1525:1542	arg1	O-glycans					1500:1508	O-glycans	1500:1508	O-glycans	1500:1508	Although this study was limited to the analysis of N-glycans and free oligosaccharides, the glycomic platform will be expanded to facilitate the analysis of O-glycans and glycans of glycosphingolipids.					
32998456	8	67	theme	glycosphingolipids	1525:1542	arg1	glycans					1514:1520	glycans	1514:1520	glycans	1514:1520	Although this study was limited to the analysis of N-glycans and free oligosaccharides, the glycomic platform will be expanded to facilitate the analysis of O-glycans and glycans of glycosphingolipids.					
32998456	5	68	theme	TAG	770:772	arg1	Expression					774:783	'TAG Expression'	769:784	'TAG Expression'	769:784	TAG consists of three units: 'TAG List' creates a glycan list that is used for database searching in TAG Expression; 'TAG Expression' automatically annotates and quantifies glycan signals and draws graphs; and 'TAG Pathway' maps the obtained expression information to biosynthetic pathways.					
32998456	6	69	theme	Chinese	1075:1081	arg1	cells					1103:1107	Chinese hamster ovary (CHO) cells	1075:1107	Chinese hamster ovary (CHO) cells	1075:1107	Herein, we discuss the concepts, outline the TAG process, and demonstrate its potential using glycomic expression profile data from Chinese hamster ovary (CHO) cells and mutants lacking a functional Npc1 gene (Npc1 knockout (KO) CHO cells).					
32683264	0	0	theme	soybean	78:84	arg1	isolate					94:100	soybean protein isolate	78:100	soybean protein isolate	78:100	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	5	1	from	contents	741:748	arg1	200 MPa					789:795	200 MPa	789:795	200 MPa	789:795	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	8	2	theme	food	1108:1111	arg1	industry					1113:1120	the food industry	1104:1120	the food industry	1104:1120	Overall, HHP represents a potential method of controlling glycation to improve protein processability and expand their applicability in the food industry.					
32683264	5	3	theme	α-helix	717:723	arg1	contents					741:748	α-helix and random coil contents	717:748	α-helix and random coil contents	717:748	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	2	4	theme	SPI	312:314	arg1	solubility					298:307	solubility	298:307	solubility of SPI	298:314	Results showed improvement in solubility of SPI upon glycation with FG.					
32683264	0	5	theme	isolate	94:100	arg1	solubility					39:48	solubility	39:48	solubility	39:48	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	0	5	theme	isolate	94:100	arg1	changes					67:73	conformation changes	54:73	conformation changes	54:73	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	0	6	from	Effect	0:5	arg1	solubility					39:48	solubility	39:48	solubility	39:48	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	0	6	from	Effect	0:5	arg1	changes					67:73	conformation changes	54:73	conformation changes	54:73	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	7	7	theme	glycated	910:917	arg1	SPI					919:921	the glycated SPI	906:921	the glycated SPI	906:921	The conformational changes in the glycated SPI supported the improved solubility of SPI-FG.					
32683264	1	8	theme	high	223:226	arg1	pressure					240:247	high hydrostatic pressure	223:247	high hydrostatic pressure (HHP 0.1-300 MPa)	223:265	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	1	8	theme	high	223:226	arg1	0.1-300 MPa					254:264	HHP 0.1-300 MPa	250:264	HHP 0.1-300 MPa	250:264	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	0	9	theme	protein	86:92	arg1	isolate					94:100	soybean protein isolate	78:100	soybean protein isolate	78:100	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	1	10	theme	hydrostatic	228:238	arg1	pressure					240:247	high hydrostatic pressure	223:247	high hydrostatic pressure (HHP 0.1-300 MPa)	223:265	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	1	10	theme	hydrostatic	228:238	arg1	0.1-300 MPa					254:264	HHP 0.1-300 MPa	250:264	HHP 0.1-300 MPa	250:264	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	8	11	theme	potential	994:1002	arg1	method					1004:1009	a potential method	992:1009	a potential method of controlling glycation to improve protein processability and expand their applicability in the food industry	992:1120	Overall, HHP represents a potential method of controlling glycation to improve protein processability and expand their applicability in the food industry.					
32683264	5	12	theme	II	778:779	arg1	band					781:784	the amide II band	768:784	the amide II band at 200 MPa	768:795	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	7	13	theme	improved	937:944	arg1	solubility					946:955	the improved solubility	933:955	the improved solubility of SPI-FG	933:965	The conformational changes in the glycated SPI supported the improved solubility of SPI-FG.					
32683264	5	14	from	vibrations	754:763	arg1	200 MPa					789:795	200 MPa	789:795	200 MPa	789:795	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	4	15	dep	occurrence	429:438	arg1	The					425:427	The	425:427	The	425:427	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	3	16	theme	maximum	344:350	arg1	solubility					352:361	The maximum solubility	340:361	The maximum solubility	340:361	The maximum solubility reached 86.84% when SPI-FG was treated at pH 8.0 and 200 MPa.					
32683264	4	17	theme	moderate	494:501	arg1	100 MPa					513:519	100 MPa	513:519	100 MPa	513:519	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	4	17	theme	moderate	494:501	arg1	pressure					503:510	moderate pressure	494:510	moderate pressure (100 MPa)	494:520	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	0	18	theme	hydrostatic	15:25	arg1	pressure					27:34	high hydrostatic pressure	10:34	high hydrostatic pressure	10:34	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	5	19	theme	secondary	641:649	arg1	structure					651:659	The secondary structure	637:659	The secondary structure of the glycated proteins	637:684	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	1	20	theme	flaxseed	179:186	arg1	gum					188:190	flaxseed gum	179:190	flaxseed gum (FG)	179:195	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	1	20	theme	flaxseed	179:186	arg1	FG					193:194	FG	193:194	FG	193:194	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	0	21	theme	high	10:13	arg1	pressure					27:34	high hydrostatic pressure	10:34	high hydrostatic pressure	10:34	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	1	22	theme	HHP	250:252	arg1	pressure					240:247	high hydrostatic pressure	223:247	high hydrostatic pressure (HHP 0.1-300 MPa)	223:265	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	1	22	theme	HHP	250:252	arg1	0.1-300 MPa					254:264	HHP 0.1-300 MPa	250:264	HHP 0.1-300 MPa	250:264	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	0	23	theme	flaxseed	116:123	arg1	gum					125:127	flaxseed gum	116:127	flaxseed gum	116:127	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	6	24	theme	microstructural	804:818	arg1	changes					820:826	These microstructural changes	798:826	These microstructural changes	798:826	These microstructural changes increased the solubility over a broad pH range.					
32683264	6	25	theme	broad	860:864	arg1	range					869:873	a broad pH range	858:873	a broad pH range	858:873	These microstructural changes increased the solubility over a broad pH range.					
32683264	4	26	theme	higher	569:574	arg1	pressures					576:584	higher pressures	569:584	higher pressures (greater than 200 MPa)	569:607	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	5	27	theme	band	781:784	arg1	vibrations					754:763	vibrations	754:763	vibrations	754:763	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	5	27	theme	band	781:784	arg1	contents					741:748	α-helix and random coil contents	717:748	α-helix and random coil contents	717:748	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	0	28	theme	pressure	27:34	arg1	Effect					0:5	Effect	0:5	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate	0:100	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	4	29	theme	SPI-FG	462:467	arg1	glycation					469:477	SPI-FG glycation	462:477	SPI-FG glycation	462:477	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	5	30	theme	random	729:734	arg1	coil					736:739	random coil	729:739	random coil	729:739	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	8	31	from	applicability	1087:1099	arg1	industry					1113:1120	the food industry	1104:1120	the food industry	1104:1120	Overall, HHP represents a potential method of controlling glycation to improve protein processability and expand their applicability in the food industry.					
32683264	7	32	theme	SPI-FG	960:965	arg1	solubility					946:955	the improved solubility	933:955	the improved solubility of SPI-FG	933:965	The conformational changes in the glycated SPI supported the improved solubility of SPI-FG.					
32683264	5	33	theme	coil	736:739	arg1	contents					741:748	α-helix and random coil contents	717:748	α-helix and random coil contents	717:748	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	5	34	theme	glycated	668:675	arg1	proteins					677:684	the glycated proteins	664:684	the glycated proteins	664:684	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	4	35	dep	pressures	576:584	arg1	greater					587:593	greater	587:593	greater	587:593	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	7	36	theme	conformational	880:893	arg1	changes					895:901	The conformational changes	876:901	The conformational changes in the glycated SPI	876:921	The conformational changes in the glycated SPI supported the improved solubility of SPI-FG.					
32683264	8	37	theme	protein	1047:1053	arg1	processability					1055:1068	protein processability	1047:1068	protein processability	1047:1068	Overall, HHP represents a potential method of controlling glycation to improve protein processability and expand their applicability in the food industry.					
32683264	4	38	theme	glycation	469:477	arg1	glycation					469:477	SPI-FG glycation	462:477	SPI-FG glycation	462:477	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	4	38	theme	glycation	469:477	arg1	degrees					441:447	degrees	441:447	degrees	441:447	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	4	38	theme	glycation	469:477	arg1	occurrence					429:438	occurrence	429:438	occurrence	429:438	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	4	38	theme	glycation	469:477	arg1	sites					453:457	sites	453:457	sites	453:457	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	1	39	theme	Soybean	130:136	arg1	isolate					146:152	Soybean protein isolate	130:152	Soybean protein isolate (SPI)	130:158	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	1	39	theme	Soybean	130:136	arg1	SPI					155:157	SPI	155:157	SPI	155:157	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	4	40	theme	Maillard	545:552	arg1	reactions					554:562	Maillard reactions	545:562	Maillard reactions	545:562	The occurrence, degrees and sites of SPI-FG glycation suggested that moderate pressure (100 MPa) significantly promoted Maillard reactions, but higher pressures (greater than 200 MPa) suppressed these reactions.					
32683264	2	41	with	glycation	321:329	arg1	FG					336:337	FG	336:337	FG	336:337	Results showed improvement in solubility of SPI upon glycation with FG.					
32683264	5	42	theme	amide	772:776	arg1	band					781:784	the amide II band	768:784	the amide II band at 200 MPa	768:795	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	1	43	theme	protein	138:144	arg1	isolate					146:152	Soybean protein isolate	130:152	Soybean protein isolate (SPI)	130:158	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	1	43	theme	protein	138:144	arg1	SPI					155:157	SPI	155:157	SPI	155:157	Soybean protein isolate (SPI) was incubated with flaxseed gum (FG) at 60 °C for 3 days under high hydrostatic pressure (HHP 0.1-300 MPa).					
32683264	0	44	theme	conformation	54:65	arg1	changes					67:73	conformation changes	54:73	conformation changes	54:73	Effect of high hydrostatic pressure on solubility and conformation changes of soybean protein isolate glycated with flaxseed gum.					
32683264	6	45	theme	pH	866:867	arg1	range					869:873	a broad pH range	858:873	a broad pH range	858:873	These microstructural changes increased the solubility over a broad pH range.					
32683264	5	46	from	200 MPa	789:795	arg1	band					781:784	the amide II band	768:784	the amide II band at 200 MPa	768:795	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	5	46	from	200 MPa	789:795	arg1	vibrations					754:763	vibrations	754:763	vibrations	754:763	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	5	46	from	200 MPa	789:795	arg1	contents					741:748	α-helix and random coil contents	717:748	α-helix and random coil contents	717:748	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
32683264	7	47	from	changes	895:901	arg1	SPI					919:921	the glycated SPI	906:921	the glycated SPI	906:921	The conformational changes in the glycated SPI supported the improved solubility of SPI-FG.					
32683264	5	48	theme	proteins	677:684	arg1	structure					651:659	The secondary structure	637:659	The secondary structure of the glycated proteins	637:684	The secondary structure of the glycated proteins varied greatly with respect to α-helix and random coil contents and vibrations of the amide II band at 200 MPa.					
34346105	0	0	theme	glycogen	97:104	arg1	enzymes					116:122	glycogen branching enzymes	97:122	glycoside hydrolase family 57 glycogen branching enzymes	67:122	Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes.					
34346105	4	1	theme	loop	747:750	arg1	tip					752:754	the loop tip	743:754	the loop tip	743:754	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	3	2	theme	gene	531:534	arg1	sequences					536:544	almost 2500 gene sequences	519:544	almost 2500 gene sequences	519:544	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	3	3	theme	sequences	536:544	arg1	analysis					507:514	a systematic sequence-based bioinformatics analysis	464:514	a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences	464:544	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	1	4	theme	Glycoside	125:133	arg1	enzymes					174:180	Glycoside hydrolase family 57 glycogen branching enzymes	125:180	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE)	125:190	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	1	4	theme	Glycoside	125:133	arg1	GH57GBE					183:189	GH57GBE	183:189	GH57GBE	183:189	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	0	5	theme	branching	106:114	arg1	enzymes					116:122	glycogen branching enzymes	97:122	glycoside hydrolase family 57 glycogen branching enzymes	67:122	Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes.					
34346105	4	6	theme	loop	723:726	arg1	β-strands					765:773	two β-strands	761:773	two β-strands	761:773	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	4	6	theme	loop	723:726	arg1	absence					701:707	the absence	697:707	the absence of a flexible loop	697:726	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	4	6	theme	loop	723:726	arg1	tyrosine					731:738	a tyrosine	729:738	a tyrosine at the loop tip	729:754	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	4	7	from	tip	752:754	arg1	β-strands					765:773	two β-strands	761:773	two β-strands	761:773	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	4	7	from	tip	752:754	arg1	absence					701:707	the absence	697:707	the absence of a flexible loop	697:726	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	4	7	from	tip	752:754	arg1	tyrosine					731:738	a tyrosine	729:738	a tyrosine at the loop tip	729:754	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	3	8	theme	family	454:459	arg1	understanding					425:437	a better understanding	416:437	a better understanding of the GH57GBE family	416:459	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	2	9	theme	atypical	294:301	arg1	number					321:326	a limited number	311:326	a limited number of GH57GBEs	311:338	As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far.					
34346105	2	9	theme	atypical	294:301	arg1	family					303:308	an atypical family	291:308	an atypical family	291:308	As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far.					
34346105	4	10	theme	no	670:671	arg1	activity					683:690	a very low or even no branching activity	651:690	a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands	651:773	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	1	11	link	linked	257:262	arg1	glucooliogosaccharides					264:285	α-1,4 linked glucooliogosaccharides	251:285	α-1,4 linked glucooliogosaccharides	251:285	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	2	12	theme	GH57GBEs	331:338	arg1	number					321:326	a limited number	311:326	a limited number of GH57GBEs	311:338	As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far.					
34346105	2	12	theme	GH57GBEs	331:338	arg1	family					303:308	an atypical family	291:308	an atypical family	291:308	As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far.					
34346105	0	13	theme	Structural	0:9	arg1	elements					11:18	Structural elements	0:18	Structural elements	0:18	Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes.					
34346105	3	14	theme	native	596:601	arg1	GH57GBEs					614:621	several native and mutant GH57GBEs	588:621	several native and mutant GH57GBEs	588:621	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	4	15	theme	branching	673:681	arg1	activity					683:690	a very low or even no branching activity	651:690	a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands	651:773	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	3	16	theme	mutant	607:612	arg1	GH57GBEs					614:621	several native and mutant GH57GBEs	588:621	several native and mutant GH57GBEs	588:621	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	3	17	theme	GH57GBE	446:452	arg1	family					454:459	the GH57GBE family	442:459	the GH57GBE family	442:459	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	0	18	dep	family	87:92	arg1	enzymes					116:122	glycogen branching enzymes	97:122	glycoside hydrolase family 57 glycogen branching enzymes	67:122	Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes.					
34346105	3	19	theme	sequence-based	477:490	arg1	analysis					507:514	a systematic sequence-based bioinformatics analysis	464:514	a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences	464:544	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	2	20	theme	limited	313:319	arg1	number					321:326	a limited number	311:326	a limited number of GH57GBEs	311:338	As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far.					
34346105	2	20	theme	limited	313:319	arg1	family					303:308	an atypical family	291:308	an atypical family	291:308	As an atypical family, a limited number of GH57GBEs have been biochemically characterized so far.					
34346105	3	21	theme	bioinformatics	492:505	arg1	analysis					507:514	a systematic sequence-based bioinformatics analysis	464:514	a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences	464:544	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	4	22	theme	flexible	714:721	arg1	loop					723:726	a flexible loop	712:726	a flexible loop	712:726	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	4	23	with	activity	683:690	arg1	tyrosine					731:738	a tyrosine	729:738	a tyrosine at the loop tip	729:754	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	4	23	with	activity	683:690	arg1	absence					701:707	the absence	697:707	the absence of a flexible loop	697:726	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	4	23	with	activity	683:690	arg1	β-strands					765:773	two β-strands	761:773	two β-strands	761:773	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	1	24	theme	α-1,6	221:225	arg1	bond					238:241	an α-1,6 glycosidic bond	218:241	an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides	218:285	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	0	25	theme	transglycosylating	36:53	arg1	activity					55:62	the transglycosylating activity	32:62	the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes	32:122	Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes.					
34346105	4	26	located	found	642:646	arg2	correlation					626:636	A correlation	624:636	A correlation	624:636	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	4	26	located	found	642:646	arg1	activity					683:690	a very low or even no branching activity	651:690	a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands	651:773	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	1	27	theme	glycosidic	227:236	arg1	bond					238:241	an α-1,6 glycosidic bond	218:241	an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides	218:285	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	0	28	theme	glycoside	67:75	arg1	family					87:92	glycoside hydrolase family 57 glycogen branching enzymes	67:122	glycoside hydrolase family 57 glycogen branching enzymes	67:122	Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes.					
34346105	3	29	theme	GH57GBEs	614:621	arg1	activity					576:583	the branching activity	562:583	the branching activity of several native and mutant GH57GBEs	562:621	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	1	30	theme	hydrolase	135:143	arg1	enzymes					174:180	Glycoside hydrolase family 57 glycogen branching enzymes	125:180	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE)	125:190	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	1	30	theme	hydrolase	135:143	arg1	GH57GBE					183:189	GH57GBE	183:189	GH57GBE	183:189	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	3	31	theme	better	418:423	arg1	understanding					425:437	a better understanding	416:437	a better understanding of the GH57GBE family	416:459	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	1	32	theme	family	145:150	arg1	enzymes					174:180	Glycoside hydrolase family 57 glycogen branching enzymes	125:180	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE)	125:190	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	1	32	theme	family	145:150	arg1	GH57GBE					183:189	GH57GBE	183:189	GH57GBE	183:189	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	0	33	gly	transglycosylating	36:53	arg0	activity					55:62	the transglycosylating activity	32:62	the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes	32:122	Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes.					
34346105	3	34	theme	several	588:594	arg1	GH57GBEs					614:621	several native and mutant GH57GBEs	588:621	several native and mutant GH57GBEs	588:621	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	1	35	theme	bond	238:241	arg1	formation					205:213	the formation	201:213	the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides	201:285	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	3	36	theme	branching	566:574	arg1	activity					576:583	the branching activity	562:583	the branching activity of several native and mutant GH57GBEs	562:621	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	1	37	theme	glycogen	155:162	arg1	enzymes					174:180	Glycoside hydrolase family 57 glycogen branching enzymes	125:180	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE)	125:190	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	1	37	theme	glycogen	155:162	arg1	GH57GBE					183:189	GH57GBE	183:189	GH57GBE	183:189	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	0	38	theme	family	87:92	arg1	activity					55:62	the transglycosylating activity	32:62	the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes	32:122	Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes.					
34346105	3	39	theme	systematic	466:475	arg1	analysis					507:514	a systematic sequence-based bioinformatics analysis	464:514	a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences	464:544	This study aimed at acquiring a better understanding of the GH57GBE family by a systematic sequence-based bioinformatics analysis of almost 2500 gene sequences and determining the branching activity of several native and mutant GH57GBEs.					
34346105	4	40	theme	low	658:660	arg1	activity					683:690	a very low or even no branching activity	651:690	a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands	651:773	A correlation was found in a very low or even no branching activity with the absence of a flexible loop, a tyrosine at the loop tip, and two β-strands.					
34346105	1	41	theme	branching	164:172	arg1	enzymes					174:180	Glycoside hydrolase family 57 glycogen branching enzymes	125:180	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE)	125:190	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	1	41	theme	branching	164:172	arg1	GH57GBE					183:189	GH57GBE	183:189	GH57GBE	183:189	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	1	42	theme	α-1,4	251:255	arg1	glucooliogosaccharides					264:285	α-1,4 linked glucooliogosaccharides	251:285	α-1,4 linked glucooliogosaccharides	251:285	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
34346105	0	43	theme	hydrolase	77:85	arg1	family					87:92	glycoside hydrolase family 57 glycogen branching enzymes	67:122	glycoside hydrolase family 57 glycogen branching enzymes	67:122	Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes.					
34346105	1	44	theme	linked	257:262	arg1	glucooliogosaccharides					264:285	α-1,4 linked glucooliogosaccharides	251:285	α-1,4 linked glucooliogosaccharides	251:285	Glycoside hydrolase family 57 glycogen branching enzymes (GH57GBE) catalyze the formation of an α-1,6 glycosidic bond between α-1,4 linked glucooliogosaccharides.					
32784723	0	0	from	Specificity	15:25	arg1	niger					64:68	Aspergillus niger	52:68	Aspergillus niger	52:68	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	3	1	theme	Aspergillus	507:517	arg1	niger					519:523	Aspergillus niger	507:523	Aspergillus niger (AnRut)	507:531	The recombinant rutinosidase from Aspergillus niger (AnRut) is selectively produced in Pichia pastoris.					
32784723	3	1	theme	Aspergillus	507:517	arg1	AnRut					526:530	AnRut	526:530	AnRut	526:530	The recombinant rutinosidase from Aspergillus niger (AnRut) is selectively produced in Pichia pastoris.					
32784723	5	2	dep	activity	770:777	arg1	addition					745:752	addition	745:752	addition	745:752	This enzyme exhibits catalytic activity towards two substrates; in addition to rutinosidase activity, it also exhibits β-d-glucopyranosidase activity.					
32784723	1	3	theme	glycosidic	232:241	arg1	bond					243:246	the glycosidic bond	228:246	the glycosidic bond between the disaccharide rutinose and the respective aglycone	228:308	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	5	4	theme	β-d-glucopyranosidase	797:817	arg1	activity					819:826	β-d-glucopyranosidase activity	797:826	β-d-glucopyranosidase activity	797:826	This enzyme exhibits catalytic activity towards two substrates; in addition to rutinosidase activity, it also exhibits β-d-glucopyranosidase activity.					
32784723	6	5	theme	inorganic	944:952	arg1	NaN3					968:971	NaN3	968:971	NaN3	968:971	As a result, new compounds are formed by β-glucosylation or rutinosylation of acceptors such as alcohols or strong inorganic nucleophiles (NaN3).					
32784723	6	5	theme	inorganic	944:952	arg1	nucleophiles					954:965	strong inorganic nucleophiles	937:965	strong inorganic nucleophiles (NaN3)	937:972	As a result, new compounds are formed by β-glucosylation or rutinosylation of acceptors such as alcohols or strong inorganic nucleophiles (NaN3).					
32784723	7	6	theme	aliphatic	1008:1016	arg1	aglycones					1018:1026	aliphatic aglycones	1008:1026	aliphatic aglycones	1008:1026	Transglycosylation products with aliphatic aglycones are resistant towards cleavage by rutinosidase, therefore, their side hydrolysis does not occur, allowing higher transglycosylation yields.					
32784723	6	7	theme	acceptors	907:915	arg1	rutinosylation					889:902	rutinosylation	889:902	rutinosylation	889:902	As a result, new compounds are formed by β-glucosylation or rutinosylation of acceptors such as alcohols or strong inorganic nucleophiles (NaN3).					
32784723	6	7	theme	acceptors	907:915	arg1	β-glucosylation					870:884	β-glucosylation	870:884	β-glucosylation	870:884	As a result, new compounds are formed by β-glucosylation or rutinosylation of acceptors such as alcohols or strong inorganic nucleophiles (NaN3).					
32784723	6	8	theme	strong	937:942	arg1	NaN3					968:971	NaN3	968:971	NaN3	968:971	As a result, new compounds are formed by β-glucosylation or rutinosylation of acceptors such as alcohols or strong inorganic nucleophiles (NaN3).					
32784723	6	8	theme	strong	937:942	arg1	nucleophiles					954:965	strong inorganic nucleophiles	937:965	strong inorganic nucleophiles (NaN3)	937:972	As a result, new compounds are formed by β-glucosylation or rutinosylation of acceptors such as alcohols or strong inorganic nucleophiles (NaN3).					
32784723	3	9	from	niger	519:523	arg1	rutinosidase					489:500	The recombinant rutinosidase	473:500	The recombinant rutinosidase from Aspergillus niger (AnRut)	473:531	The recombinant rutinosidase from Aspergillus niger (AnRut) is selectively produced in Pichia pastoris.					
32784723	10	10	theme	transglycosylation	1346:1363	arg1	products					1365:1372	the transglycosylation products	1342:1372	the transglycosylation products	1342:1372	Interactions between the transglycosylation products and the recombinant AnRut were analyzed by molecular modeling.					
32784723	4	11	theme	transglycosylation	593:610	arg1	preparation					639:649	an unpurified preparation	625:649	an unpurified preparation directly from cultivation	625:675	It can catalyze transglycosylation reactions as an unpurified preparation directly from cultivation.					
32784723	4	11	theme	transglycosylation	593:610	arg1	reactions					612:620	transglycosylation reactions	593:620	transglycosylation reactions	593:620	It can catalyze transglycosylation reactions as an unpurified preparation directly from cultivation.					
32784723	5	12	theme	rutinosidase	757:768	arg1	activity					770:777	rutinosidase activity	757:777	rutinosidase activity	757:777	This enzyme exhibits catalytic activity towards two substrates; in addition to rutinosidase activity, it also exhibits β-d-glucopyranosidase activity.					
32784723	2	13	with	acceptors	409:417	arg1	group					435:439	a free -OH group	424:439	a free -OH group (so-called transglycosylation)	424:470	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	2	13	with	acceptors	409:417	arg1	transglycosylation					452:469	so-called transglycosylation	442:469	so-called transglycosylation	442:469	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	11	14	theme	catalytic	1535:1543	arg1	properties					1545:1554	the unusual catalytic properties	1523:1554	the unusual catalytic properties of this glycosidase	1523:1574	We revealed the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.					
32784723	3	15	theme	recombinant	477:487	arg1	rutinosidase					489:500	The recombinant rutinosidase	473:500	The recombinant rutinosidase from Aspergillus niger (AnRut)	473:531	The recombinant rutinosidase from Aspergillus niger (AnRut) is selectively produced in Pichia pastoris.					
32784723	0	16	theme	Tunnel	100:105	arg1	Specificity					15:25	Dual Substrate Specificity	0:25	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger	0:68	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	0	16	theme	Tunnel	100:105	arg1	Role					78:81	the Role	74:81	the Role of Its Substrate Tunnel	74:105	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	7	17	theme	transglycosylation	1141:1158	arg1	yields					1160:1165	higher transglycosylation yields	1134:1165	higher transglycosylation yields	1134:1165	Transglycosylation products with aliphatic aglycones are resistant towards cleavage by rutinosidase, therefore, their side hydrolysis does not occur, allowing higher transglycosylation yields.					
32784723	0	18	theme	Substrate	90:98	arg1	Tunnel					100:105	Its Substrate Tunnel	86:105	Its Substrate Tunnel	86:105	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	11	19	theme	substrate	1463:1471	arg1	tunnel					1473:1478	a substrate tunnel	1461:1478	a substrate tunnel	1461:1478	We revealed the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.					
32784723	12	20	theme	biosynthetic	1647:1658	arg1	applications					1660:1671	biosynthetic applications	1647:1671	biosynthetic applications	1647:1671	AnRut is attractive for biosynthetic applications, especially for the use of inexpensive substrates (rutin and isoquercitrin).					
32784723	1	21	dep	Rutinosidases	108:120	arg1	3.2.1.168					176:184	EC 3.2.1.168	173:184	EC 3.2.1.168	173:184	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	1	21	dep	Rutinosidases	108:120	arg1	GH5					192:194	CAZy GH5	187:194	CAZy GH5	187:194	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	1	21	dep	Rutinosidases	108:120	arg1	-β-d-glucopyranosidases					148:170	α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases	123:170	α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases	123:170	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	1	22	theme	EC	173:174	arg1	3.2.1.168					176:184	EC 3.2.1.168	173:184	EC 3.2.1.168	173:184	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	1	22	theme	EC	173:174	arg1	-β-d-glucopyranosidases					148:170	α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases	123:170	α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases	123:170	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	0	23	theme	Substrate	5:13	arg1	Specificity					15:25	Dual Substrate Specificity	0:25	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger	0:68	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	11	24	from	role	1453:1456	arg1	structure					1487:1495	the structure	1483:1495	the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential	1483:1620	We revealed the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.					
32784723	12	25	dep	substrates	1712:1721	arg1	substrates					1712:1721	inexpensive substrates	1700:1721	inexpensive substrates (rutin and isoquercitrin)	1700:1747	AnRut is attractive for biosynthetic applications, especially for the use of inexpensive substrates (rutin and isoquercitrin).					
32784723	12	25	dep	substrates	1712:1721	arg1	isoquercitrin					1734:1746	isoquercitrin	1734:1746	isoquercitrin	1734:1746	AnRut is attractive for biosynthetic applications, especially for the use of inexpensive substrates (rutin and isoquercitrin).					
32784723	12	25	dep	substrates	1712:1721	arg1	rutin					1724:1728	rutin	1724:1728	rutin	1724:1728	AnRut is attractive for biosynthetic applications, especially for the use of inexpensive substrates (rutin and isoquercitrin).					
32784723	7	26	theme	Transglycosylation	975:992	arg1	products					994:1001	Transglycosylation products	975:1001	Transglycosylation products with aliphatic aglycones	975:1026	Transglycosylation products with aliphatic aglycones are resistant towards cleavage by rutinosidase, therefore, their side hydrolysis does not occur, allowing higher transglycosylation yields.					
32784723	10	27	theme	recombinant	1382:1392	arg1	AnRut					1394:1398	the recombinant AnRut	1378:1398	the recombinant AnRut	1378:1398	Interactions between the transglycosylation products and the recombinant AnRut were analyzed by molecular modeling.					
32784723	0	28	theme	Dual	0:3	arg1	Specificity					15:25	Dual Substrate Specificity	0:25	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger	0:68	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	1	29	theme	disaccharide	260:271	arg1	rutinose					273:280	the disaccharide rutinose	256:280	the disaccharide rutinose	256:280	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	7	30	theme	higher	1134:1139	arg1	yields					1160:1165	higher transglycosylation yields	1134:1165	higher transglycosylation yields	1134:1165	Transglycosylation products with aliphatic aglycones are resistant towards cleavage by rutinosidase, therefore, their side hydrolysis does not occur, allowing higher transglycosylation yields.					
32784723	4	31	from	cultivation	665:675	arg1	preparation					639:649	an unpurified preparation	625:649	an unpurified preparation directly from cultivation	625:675	It can catalyze transglycosylation reactions as an unpurified preparation directly from cultivation.					
32784723	4	31	from	cultivation	665:675	arg1	reactions					612:620	transglycosylation reactions	593:620	transglycosylation reactions	593:620	It can catalyze transglycosylation reactions as an unpurified preparation directly from cultivation.					
32784723	2	32	theme	-OH	431:433	arg1	group					435:439	a free -OH group	424:439	a free -OH group (so-called transglycosylation)	424:470	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	2	32	theme	-OH	431:433	arg1	transglycosylation					452:469	so-called transglycosylation	442:469	so-called transglycosylation	442:469	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	5	33	dep	exhibits	788:795	arg1	exhibits					690:697	exhibits	690:697	exhibits catalytic activity towards two substrates	690:739	This enzyme exhibits catalytic activity towards two substrates; in addition to rutinosidase activity, it also exhibits β-d-glucopyranosidase activity.					
32784723	1	34	theme	CAZy	187:190	arg1	GH5					192:194	CAZy GH5	187:194	CAZy GH5	187:194	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	1	34	theme	CAZy	187:190	arg1	-β-d-glucopyranosidases					148:170	α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases	123:170	α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases	123:170	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	2	35	theme	free	426:429	arg1	group					435:439	a free -OH group	424:439	a free -OH group (so-called transglycosylation)	424:470	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	2	35	theme	free	426:429	arg1	transglycosylation					452:469	so-called transglycosylation	442:469	so-called transglycosylation	442:469	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	0	36	from	Role	78:81	arg1	niger					64:68	Aspergillus niger	52:68	Aspergillus niger	52:68	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	3	37	theme	Pichia	560:565	arg1	pastoris					567:574	Pichia pastoris	560:574	Pichia pastoris	560:574	The recombinant rutinosidase from Aspergillus niger (AnRut) is selectively produced in Pichia pastoris.					
32784723	10	38	theme	molecular	1417:1425	arg1	modeling					1427:1434	molecular modeling	1417:1434	molecular modeling	1417:1434	Interactions between the transglycosylation products and the recombinant AnRut were analyzed by molecular modeling.					
32784723	11	39	theme	glycosidase	1564:1574	arg1	potential					1612:1620	its specific transglycosylation potential	1580:1620	its specific transglycosylation potential	1580:1620	We revealed the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.					
32784723	11	39	theme	glycosidase	1564:1574	arg1	properties					1545:1554	the unusual catalytic properties	1523:1554	the unusual catalytic properties of this glycosidase	1523:1574	We revealed the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.					
32784723	0	40	theme	Rutinosidase	34:45	arg1	Specificity					15:25	Dual Substrate Specificity	0:25	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger	0:68	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	0	40	theme	Rutinosidase	34:45	arg1	Role					78:81	the Role	74:81	the Role of Its Substrate Tunnel	74:105	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	11	41	theme	specific	1584:1591	arg1	potential					1612:1620	its specific transglycosylation potential	1580:1620	its specific transglycosylation potential	1580:1620	We revealed the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.					
32784723	11	42	theme	tunnel	1473:1478	arg1	role					1453:1456	the role	1449:1456	the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential	1449:1620	We revealed the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.					
32784723	1	43	theme	respective	290:299	arg1	aglycone					301:308	the respective aglycone	286:308	the respective aglycone	286:308	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	8	44	theme	selected	1242:1249	arg1	acceptors					1251:1259	selected acceptors	1242:1259	selected acceptors	1242:1259	Fourteen compounds were synthesized by glucosylation or rutinosylation of selected acceptors.					
32784723	0	45	theme	Aspergillus	52:62	arg1	niger					64:68	Aspergillus niger	52:68	Aspergillus niger	52:68	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	7	46	with	products	994:1001	arg1	aglycones					1018:1026	aliphatic aglycones	1008:1026	aliphatic aglycones	1008:1026	Transglycosylation products with aliphatic aglycones are resistant towards cleavage by rutinosidase, therefore, their side hydrolysis does not occur, allowing higher transglycosylation yields.					
32784723	5	47	theme	catalytic	699:707	arg1	activity					709:716	catalytic activity	699:716	catalytic activity towards two substrates	699:739	This enzyme exhibits catalytic activity towards two substrates; in addition to rutinosidase activity, it also exhibits β-d-glucopyranosidase activity.					
32784723	0	48	from	niger	64:68	arg1	Specificity					15:25	Dual Substrate Specificity	0:25	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger	0:68	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	0	48	from	niger	64:68	arg1	Rutinosidase					34:45	the Rutinosidase	30:45	the Rutinosidase from Aspergillus niger	30:68	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	0	48	from	niger	64:68	arg1	Role					78:81	the Role	74:81	the Role of Its Substrate Tunnel	74:105	Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel.					
32784723	2	49	theme	so-called	442:450	arg1	group					435:439	a free -OH group	424:439	a free -OH group (so-called transglycosylation)	424:470	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	2	49	theme	so-called	442:450	arg1	transglycosylation					452:469	so-called transglycosylation	442:469	so-called transglycosylation	442:469	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	11	50	theme	unusual	1527:1533	arg1	properties					1545:1554	the unusual catalytic properties	1523:1554	the unusual catalytic properties of this glycosidase	1523:1574	We revealed the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.					
32784723	12	51	theme	inexpensive	1700:1710	arg1	substrates					1712:1721	inexpensive substrates	1700:1721	inexpensive substrates (rutin and isoquercitrin)	1700:1747	AnRut is attractive for biosynthetic applications, especially for the use of inexpensive substrates (rutin and isoquercitrin).					
32784723	12	51	theme	inexpensive	1700:1710	arg1	isoquercitrin					1734:1746	isoquercitrin	1734:1746	isoquercitrin	1734:1746	AnRut is attractive for biosynthetic applications, especially for the use of inexpensive substrates (rutin and isoquercitrin).					
32784723	12	51	theme	inexpensive	1700:1710	arg1	rutin					1724:1728	rutin	1724:1728	rutin	1724:1728	AnRut is attractive for biosynthetic applications, especially for the use of inexpensive substrates (rutin and isoquercitrin).					
32784723	2	52	theme	retaining	327:335	arg1	glycosidases					337:348	many retaining glycosidases	322:348	many retaining glycosidases	322:348	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	11	53	theme	transglycosylation	1593:1610	arg1	potential					1612:1620	its specific transglycosylation potential	1580:1620	its specific transglycosylation potential	1580:1620	We revealed the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.					
32784723	12	54	theme	substrates	1712:1721	arg1	use					1693:1695	the use	1689:1695	the use of inexpensive substrates (rutin and isoquercitrin)	1689:1747	AnRut is attractive for biosynthetic applications, especially for the use of inexpensive substrates (rutin and isoquercitrin).					
32784723	6	55	theme	new	842:844	arg1	result					834:839	a result	832:839	a result	832:839	As a result, new compounds are formed by β-glucosylation or rutinosylation of acceptors such as alcohols or strong inorganic nucleophiles (NaN3).					
32784723	6	55	theme	new	842:844	arg1	compounds					846:854	new compounds	842:854	new compounds	842:854	As a result, new compounds are formed by β-glucosylation or rutinosylation of acceptors such as alcohols or strong inorganic nucleophiles (NaN3).					
32784723	2	56	theme	many	322:325	arg1	glycosidases					337:348	many retaining glycosidases	322:348	many retaining glycosidases	322:348	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	1	57	theme	α-l-rhamnopyranosyl-	123:142	arg1	3.2.1.168					176:184	EC 3.2.1.168	173:184	EC 3.2.1.168	173:184	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	1	57	theme	α-l-rhamnopyranosyl-	123:142	arg1	GH5					192:194	CAZy GH5	187:194	CAZy GH5	187:194	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	1	57	theme	α-l-rhamnopyranosyl-	123:142	arg1	-β-d-glucopyranosidases					148:170	α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases	123:170	α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases	123:170	Rutinosidases (α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranosidases, EC 3.2.1.168, CAZy GH5) are diglycosidases that cleave the glycosidic bond between the disaccharide rutinose and the respective aglycone.					
32784723	7	58	theme	side	1093:1096	arg1	hydrolysis					1098:1107	their side hydrolysis	1087:1107	their side hydrolysis	1087:1107	Transglycosylation products with aliphatic aglycones are resistant towards cleavage by rutinosidase, therefore, their side hydrolysis does not occur, allowing higher transglycosylation yields.					
32784723	8	59	theme	acceptors	1251:1259	arg1	glucosylation					1207:1219	glucosylation	1207:1219	glucosylation	1207:1219	Fourteen compounds were synthesized by glucosylation or rutinosylation of selected acceptors.					
32784723	8	59	theme	acceptors	1251:1259	arg1	rutinosylation					1224:1237	rutinosylation	1224:1237	rutinosylation	1224:1237	Fourteen compounds were synthesized by glucosylation or rutinosylation of selected acceptors.					
32784723	2	60	theme	rutinosyl	387:395	arg1	moiety					397:402	the rutinosyl moiety	383:402	the rutinosyl moiety	383:402	Similar to many retaining glycosidases, rutinosidases can also transfer the rutinosyl moiety onto acceptors with a free -OH group (so-called transglycosylation).					
32784723	4	61	theme	unpurified	628:637	arg1	preparation					639:649	an unpurified preparation	625:649	an unpurified preparation directly from cultivation	625:675	It can catalyze transglycosylation reactions as an unpurified preparation directly from cultivation.					
32784723	4	61	theme	unpurified	628:637	arg1	reactions					612:620	transglycosylation reactions	593:620	transglycosylation reactions	593:620	It can catalyze transglycosylation reactions as an unpurified preparation directly from cultivation.					
32784723	11	62	theme	AnRut	1500:1504	arg1	structure					1487:1495	the structure	1483:1495	the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential	1483:1620	We revealed the role of a substrate tunnel in the structure of AnRut, which explained the unusual catalytic properties of this glycosidase and its specific transglycosylation potential.					
33547931	1	0	from	glycoproteins	276:288	arg1	report					318:323	the first report	308:323	the first report of OsXylT/OsFucT double KO mutation in rice	308:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	1	0	from	glycoproteins	276:288	arg1	rice					293:296	rice	293:296	rice	293:296	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	1	0	from	glycoproteins	276:288	arg1	elimination					206:216	the elimination	202:216	the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice	202:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	1	1	gly	glycoproteins	276:288	arg1	glycoproteins					276:288	glycoproteins	276:288	glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice	276:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	5	2	theme	N-glycan	889:896	arg1	genes					898:902	the plant-specific N-glycan genes	870:902	the plant-specific N-glycan genes of rice (Oryza sativa)	870:925	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	5	2	theme	N-glycan	889:896	arg1	Oryza					913:917	Oryza	913:917	Oryza	913:917	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	9	3	theme	other	1371:1375	arg1	α1,4-fucose					1423:1433	α1,4-fucose	1423:1433	α1,4-fucose	1423:1433	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	9	3	theme	other	1371:1375	arg1	β1,3-galactose					1404:1417	β1,3-galactose	1404:1417	β1,3-galactose	1404:1417	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	9	3	theme	other	1371:1375	arg1	residues					1392:1399	other plant-specific residues	1371:1399	other plant-specific residues of β1,3-galactose and α1,4-fucose	1371:1433	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	4	4	gly	glycoproteins	690:702	arg1	glycoproteins					690:702	glycoproteins	690:702	glycoproteins	690:702	In plant-specific manner, β1,2-xylose and α1,3-fucose residues are transferred to N-glycan core structure on glycoproteins by β1,2-xylosyltransferase (β1,2-XylT) and α1,3-fucosyltransferase (α1,3-FucT), respectively.					
33547931	10	5	theme	OsFucT	1621:1626	arg1	knock-out					1592:1600	the double knock-out	1581:1600	the double knock-out of both OsXylT and OsFucT	1581:1626	Thus, we suggest that each enzymes working on the process for complex N-glycan biosynthesis might independently act in rice, hence the double knock-out of both OsXylT and OsFucT might be not enough to humanize N-glycan structure in rice.					
33547931	8	6	from	N-glycans	1258:1266	arg1	analysis					1284:1291	MALDI-TOF/MS analysis	1271:1291	MALDI-TOF/MS analysis	1271:1291	Consistently, there was no peak corresponding to the N-glycans in MALDI-TOF/MS analysis.					
33547931	4	7	theme	core	672:675	arg1	structure					677:685	N-glycan core structure	663:685	N-glycan core structure on glycoproteins	663:702	In plant-specific manner, β1,2-xylose and α1,3-fucose residues are transferred to N-glycan core structure on glycoproteins by β1,2-xylosyltransferase (β1,2-XylT) and α1,3-fucosyltransferase (α1,3-FucT), respectively.					
33547931	6	8	contain	have	1069:1072	arg1	lines					1049:1053	The double knock-out lines	1028:1053	The double knock-out lines	1028:1053	The double knock-out lines were found to have frameshift mutations by INDELs.					
33547931	6	8	contain	have	1069:1072	arg2	mutations					1085:1093	frameshift mutations	1074:1093	frameshift mutations	1074:1093	The double knock-out lines were found to have frameshift mutations by INDELs.					
33547931	0	9	from	Inactivation	0:11	arg1	rice					92:95	rice	92:95	rice	92:95	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	0	10	theme	multiplex	115:123	arg1	strategy					137:144	multiplex CRISPR/Cas9 strategy	115:144	multiplex CRISPR/Cas9 strategy	115:144	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	9	11	theme	plant-specific	1377:1390	arg1	α1,4-fucose					1423:1433	α1,4-fucose	1423:1433	α1,4-fucose	1423:1433	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	9	11	theme	plant-specific	1377:1390	arg1	β1,3-galactose					1404:1417	β1,3-galactose	1404:1417	β1,3-galactose	1404:1417	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	9	11	theme	plant-specific	1377:1390	arg1	residues					1392:1399	other plant-specific residues	1371:1399	other plant-specific residues of β1,3-galactose and α1,4-fucose	1371:1433	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	7	12	located	detected	1170:1177	arg1	analysis					1195:1202	Western blot analysis	1182:1202	Western blot analysis	1182:1202	Both β1,2-xylose and α1,3-fucose residues in the lines were not detected in Western blot analysis.					
33547931	7	12	located	detected	1170:1177	arg2	residues					1139:1146	Both β1,2-xylose and α1,3-fucose residues	1106:1146	Both β1,2-xylose and α1,3-fucose residues in the lines	1106:1159	Both β1,2-xylose and α1,3-fucose residues in the lines were not detected in Western blot analysis.					
33547931	0	13	from	rice	92:95	arg1	Inactivation					0:11	Inactivation	0:11	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.	0:145	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	0	14	theme	α	56:56	arg1	sativa					104:109	Oryza sativa	98:109	Oryza sativa	98:109	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	0	14	theme	α	56:56	arg1	gene					84:87	the α (1, 3)-fucosyltransferase gene	52:87	gene	84:87	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	5	15	dep	effort	804:809	arg1	use					814:816	use	814:816	to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT)	811:968	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	10	16	theme	double	1585:1590	arg1	knock-out					1592:1600	the double knock-out	1581:1600	the double knock-out of both OsXylT and OsFucT	1581:1626	Thus, we suggest that each enzymes working on the process for complex N-glycan biosynthesis might independently act in rice, hence the double knock-out of both OsXylT and OsFucT might be not enough to humanize N-glycan structure in rice.					
33547931	5	17	dep	Oryza	913:917	arg1	sativa					919:924	Oryza sativa	913:924	Oryza sativa	913:924	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	1	18	theme	first	312:316	arg1	rice					293:296	rice	293:296	rice	293:296	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	1	18	theme	first	312:316	arg1	report					318:323	the first report	308:323	the first report of OsXylT/OsFucT double KO mutation in rice	308:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	10	19	theme	complex	1512:1518	arg1	biosynthesis					1529:1540	complex N-glycan biosynthesis	1512:1540	complex N-glycan biosynthesis	1512:1540	Thus, we suggest that each enzymes working on the process for complex N-glycan biosynthesis might independently act in rice, hence the double knock-out of both OsXylT and OsFucT might be not enough to humanize N-glycan structure in rice.					
33547931	10	20	theme	OsXylT	1610:1615	arg1	knock-out					1592:1600	the double knock-out	1581:1600	the double knock-out of both OsXylT and OsFucT	1581:1626	Thus, we suggest that each enzymes working on the process for complex N-glycan biosynthesis might independently act in rice, hence the double knock-out of both OsXylT and OsFucT might be not enough to humanize N-glycan structure in rice.					
33547931	6	21	theme	knock-out	1039:1047	arg1	lines					1049:1053	The double knock-out lines	1028:1053	The double knock-out lines	1028:1053	The double knock-out lines were found to have frameshift mutations by INDELs.					
33547931	7	22	from	residues	1139:1146	arg1	lines					1155:1159	the lines	1151:1159	the lines	1151:1159	Both β1,2-xylose and α1,3-fucose residues in the lines were not detected in Western blot analysis.					
33547931	0	23	theme	CRISPR/Cas9	125:135	arg1	strategy					137:144	multiplex CRISPR/Cas9 strategy	115:144	multiplex CRISPR/Cas9 strategy	115:144	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	10	24	theme	N-glycan	1520:1527	arg1	biosynthesis					1529:1540	complex N-glycan biosynthesis	1512:1540	complex N-glycan biosynthesis	1512:1540	Thus, we suggest that each enzymes working on the process for complex N-glycan biosynthesis might independently act in rice, hence the double knock-out of both OsXylT and OsFucT might be not enough to humanize N-glycan structure in rice.					
33547931	4	25	theme	α1,3-fucose	623:633	arg1	residues					635:642	β1,2-xylose and α1,3-fucose residues	607:642	β1,2-xylose and α1,3-fucose residues	607:642	In plant-specific manner, β1,2-xylose and α1,3-fucose residues are transferred to N-glycan core structure on glycoproteins by β1,2-xylosyltransferase (β1,2-XylT) and α1,3-fucosyltransferase (α1,3-FucT), respectively.					
33547931	1	26	from	report	318:323	arg1	rice					364:367	rice	364:367	rice	364:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	9	27	theme	β1,2-xylose	1319:1329	arg1	residues					1331:1338	α1,3-fucose and β1,2-xylose residues	1303:1338	α1,3-fucose and β1,2-xylose residues	1303:1338	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	7	28	theme	Western	1182:1188	arg1	analysis					1195:1202	Western blot analysis	1182:1202	Western blot analysis	1182:1202	Both β1,2-xylose and α1,3-fucose residues in the lines were not detected in Western blot analysis.					
33547931	9	29	theme	α1,4-fucose	1423:1433	arg1	α1,4-fucose					1423:1433	α1,4-fucose	1423:1433	α1,4-fucose	1423:1433	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	9	29	theme	α1,4-fucose	1423:1433	arg1	β1,3-galactose					1404:1417	β1,3-galactose	1404:1417	β1,3-galactose	1404:1417	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	9	29	theme	α1,4-fucose	1423:1433	arg1	residues					1392:1399	other plant-specific residues	1371:1399	other plant-specific residues of β1,3-galactose and α1,4-fucose	1371:1433	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	9	30	theme	α1,3-fucose	1303:1313	arg1	residues					1331:1338	α1,3-fucose and β1,2-xylose residues	1303:1338	α1,3-fucose and β1,2-xylose residues	1303:1338	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	1	31	theme	plant-specific	221:234	arg1	residues					264:271	plant-specific β1,2-xylose and α1,3-fucose residues	221:271	plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice	221:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	0	32	theme	β	20:20	arg1	-xylosyltransferase					28:46	the β (1, 2)-xylosyltransferase	16:46	-xylosyltransferase	28:46	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	1	33	theme	β1,2-xylose	236:246	arg1	residues					264:271	plant-specific β1,2-xylose and α1,3-fucose residues	221:271	plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice	221:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	1	34	from	elimination	206:216	arg1	glycoproteins					276:288	glycoproteins	276:288	glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice	276:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	9	35	theme	β1,3-galactose	1404:1417	arg1	α1,4-fucose					1423:1433	α1,4-fucose	1423:1433	α1,4-fucose	1423:1433	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	9	35	theme	β1,3-galactose	1404:1417	arg1	β1,3-galactose					1404:1417	β1,3-galactose	1404:1417	β1,3-galactose	1404:1417	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	9	35	theme	β1,3-galactose	1404:1417	arg1	residues					1392:1399	other plant-specific residues	1371:1399	other plant-specific residues of β1,3-galactose and α1,4-fucose	1371:1433	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	1	36	from	residues	264:271	arg1	glycoproteins					276:288	glycoproteins	276:288	glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice	276:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	5	37	theme	rice	907:910	arg1	OsFucT					962:967	OsFucT	962:967	OsFucT	962:967	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	5	37	theme	rice	907:910	arg1	Oryza					913:917	Oryza	913:917	Oryza	913:917	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	5	37	theme	rice	907:910	arg1	OsXylT					939:944	OsXylT	939:944	OsXylT	939:944	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	5	37	theme	rice	907:910	arg1	genes					898:902	the plant-specific N-glycan genes	870:902	the plant-specific N-glycan genes of rice (Oryza sativa)	870:925	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	5	37	theme	rice	907:910	arg1	α1,3-FucT					951:959	α1,3-FucT	951:959	α1,3-FucT (OsFucT)	951:968	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	5	37	theme	rice	907:910	arg1	β1,2-xylT					928:936	β1,2-xylT	928:936	β1,2-xylT (OsXylT)	928:945	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	5	37	theme	rice	907:910	arg1	biopharmaceuticals					850:867	biopharmaceuticals	850:867	biopharmaceuticals	850:867	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	2	38	dep	conserved	460:468	arg1	as					450:451	as	450:451	as	450:451	N-glycosylation pathway is the one of post-translational mechanism and is known as highly conserved in eukaryotes.					
33547931	1	39	theme	OsXylT/OsFucT	328:340	arg1	mutation					352:359	OsXylT/OsFucT double KO mutation	328:359	OsXylT/OsFucT double KO mutation	328:359	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	3	40	theme	complex-N-glycan	510:525	arg1	modification					527:538	complex-N-glycan modification	510:538	complex-N-glycan modification	510:538	However, the process for complex-N-glycan modification is different between mammals and plants.					
33547931	7	41	theme	β1,2-xylose	1111:1121	arg1	residues					1139:1146	Both β1,2-xylose and α1,3-fucose residues	1106:1146	Both β1,2-xylose and α1,3-fucose residues in the lines	1106:1159	Both β1,2-xylose and α1,3-fucose residues in the lines were not detected in Western blot analysis.					
33547931	8	42	theme	MALDI-TOF/MS	1271:1282	arg1	analysis					1284:1291	MALDI-TOF/MS analysis	1271:1291	MALDI-TOF/MS analysis	1271:1291	Consistently, there was no peak corresponding to the N-glycans in MALDI-TOF/MS analysis.					
33547931	1	43	theme	α1,3-fucose	252:262	arg1	residues					264:271	plant-specific β1,2-xylose and α1,3-fucose residues	221:271	plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice	221:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	0	44	theme	gene	84:87	arg1	Inactivation					0:11	Inactivation	0:11	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.	0:145	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	1	45	theme	double	342:347	arg1	mutation					352:359	OsXylT/OsFucT double KO mutation	328:359	OsXylT/OsFucT double KO mutation	328:359	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	2	46	theme	mechanism	427:435	arg1	pathway					386:392	N-glycosylation pathway	370:392	N-glycosylation pathway	370:392	N-glycosylation pathway is the one of post-translational mechanism and is known as highly conserved in eukaryotes.					
33547931	2	46	theme	mechanism	427:435	arg1	one					401:403	one	401:403	one	401:403	N-glycosylation pathway is the one of post-translational mechanism and is known as highly conserved in eukaryotes.					
33547931	2	46	theme	mechanism	427:435	arg1	mechanism					427:435	post-translational mechanism	408:435	post-translational mechanism	408:435	N-glycosylation pathway is the one of post-translational mechanism and is known as highly conserved in eukaryotes.					
33547931	1	47	theme	residues	264:271	arg1	elimination					206:216	the elimination	202:216	the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice	202:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	0	48	theme	-fucosyltransferase	64:82	arg1	sativa					104:109	Oryza sativa	98:109	Oryza sativa	98:109	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	0	48	theme	-fucosyltransferase	64:82	arg1	gene					84:87	the α (1, 3)-fucosyltransferase gene	52:87	gene	84:87	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	0	49	from	-xylosyltransferase	28:46	arg1	rice					92:95	rice	92:95	rice	92:95	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	4	50	theme	plant-specific	584:597	arg1	manner					599:604	plant-specific manner	584:604	plant-specific manner	584:604	In plant-specific manner, β1,2-xylose and α1,3-fucose residues are transferred to N-glycan core structure on glycoproteins by β1,2-xylosyltransferase (β1,2-XylT) and α1,3-fucosyltransferase (α1,3-FucT), respectively.					
33547931	1	51	theme	CRISPR/Cas9-mediated	147:166	arg1	OsXylT					168:173	CRISPR/Cas9-mediated OsXylT	147:173	CRISPR/Cas9-mediated OsXylT	147:173	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	2	52	theme	post-translational	408:425	arg1	mechanism					427:435	post-translational mechanism	408:435	post-translational mechanism	408:435	N-glycosylation pathway is the one of post-translational mechanism and is known as highly conserved in eukaryotes.					
33547931	1	53	theme	mutation	352:359	arg1	rice					293:296	rice	293:296	rice	293:296	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	1	53	theme	mutation	352:359	arg1	report					318:323	the first report	308:323	the first report of OsXylT/OsFucT double KO mutation in rice	308:367	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	10	54	dep	enough	1641:1646	arg1	not					1637:1639	not	1637:1639	not	1637:1639	Thus, we suggest that each enzymes working on the process for complex N-glycan biosynthesis might independently act in rice, hence the double knock-out of both OsXylT and OsFucT might be not enough to humanize N-glycan structure in rice.					
33547931	10	55	dep	enzymes	1477:1483	arg1	working					1485:1491	working	1485:1491	working	1485:1491	Thus, we suggest that each enzymes working on the process for complex N-glycan biosynthesis might independently act in rice, hence the double knock-out of both OsXylT and OsFucT might be not enough to humanize N-glycan structure in rice.					
33547931	10	56	theme	N-glycan	1660:1667	arg1	structure					1669:1677	N-glycan structure	1660:1677	N-glycan structure	1660:1677	Thus, we suggest that each enzymes working on the process for complex N-glycan biosynthesis might independently act in rice, hence the double knock-out of both OsXylT and OsFucT might be not enough to humanize N-glycan structure in rice.					
33547931	1	57	theme	KO	349:350	arg1	mutation					352:359	OsXylT/OsFucT double KO mutation	328:359	OsXylT/OsFucT double KO mutation	328:359	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	0	58	theme	Oryza	98:102	arg1	sativa					104:109	Oryza sativa	98:109	Oryza sativa	98:109	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	0	58	theme	Oryza	98:102	arg1	gene					84:87	the α (1, 3)-fucosyltransferase gene	52:87	gene	84:87	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	5	59	theme	multiplex	994:1002	arg1	technology					1016:1025	multiplex CRISPR/Cas9 technology	994:1025	multiplex CRISPR/Cas9 technology	994:1025	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	6	60	theme	frameshift	1074:1083	arg1	mutations					1085:1093	frameshift mutations	1074:1093	frameshift mutations	1074:1093	The double knock-out lines were found to have frameshift mutations by INDELs.					
33547931	0	61	from	gene	84:87	arg1	rice					92:95	rice	92:95	rice	92:95	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	5	62	theme	CRISPR/Cas9	1004:1014	arg1	technology					1016:1025	multiplex CRISPR/Cas9 technology	994:1025	multiplex CRISPR/Cas9 technology	994:1025	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	0	63	theme	-xylosyltransferase	28:46	arg1	Inactivation					0:11	Inactivation	0:11	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.	0:145	Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.					
33547931	4	64	theme	β1,2-xylose	607:617	arg1	residues					635:642	β1,2-xylose and α1,3-fucose residues	607:642	β1,2-xylose and α1,3-fucose residues	607:642	In plant-specific manner, β1,2-xylose and α1,3-fucose residues are transferred to N-glycan core structure on glycoproteins by β1,2-xylosyltransferase (β1,2-XylT) and α1,3-fucosyltransferase (α1,3-FucT), respectively.					
33547931	2	65	theme	N-glycosylation	370:384	arg1	pathway					386:392	N-glycosylation pathway	370:392	N-glycosylation pathway	370:392	N-glycosylation pathway is the one of post-translational mechanism and is known as highly conserved in eukaryotes.					
33547931	2	65	theme	N-glycosylation	370:384	arg1	one					401:403	one	401:403	one	401:403	N-glycosylation pathway is the one of post-translational mechanism and is known as highly conserved in eukaryotes.					
33547931	2	65	theme	N-glycosylation	370:384	arg1	mechanism					427:435	post-translational mechanism	408:435	post-translational mechanism	408:435	N-glycosylation pathway is the one of post-translational mechanism and is known as highly conserved in eukaryotes.					
33547931	4	66	theme	N-glycan	663:670	arg1	structure					677:685	N-glycan core structure	663:685	N-glycan core structure on glycoproteins	663:702	In plant-specific manner, β1,2-xylose and α1,3-fucose residues are transferred to N-glycan core structure on glycoproteins by β1,2-xylosyltransferase (β1,2-XylT) and α1,3-fucosyltransferase (α1,3-FucT), respectively.					
33547931	6	67	theme	double	1032:1037	arg1	lines					1049:1053	The double knock-out lines	1028:1053	The double knock-out lines	1028:1053	The double knock-out lines were found to have frameshift mutations by INDELs.					
33547931	1	68	theme	OsFucT	179:184	arg1	mutation					186:193	OsFucT mutation	179:193	OsFucT mutation	179:193	CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice.					
33547931	7	69	theme	blot	1190:1193	arg1	analysis					1195:1202	Western blot analysis	1182:1202	Western blot analysis	1182:1202	Both β1,2-xylose and α1,3-fucose residues in the lines were not detected in Western blot analysis.					
33547931	7	70	theme	α1,3-fucose	1127:1137	arg1	residues					1139:1146	Both β1,2-xylose and α1,3-fucose residues	1106:1146	Both β1,2-xylose and α1,3-fucose residues in the lines	1106:1159	Both β1,2-xylose and α1,3-fucose residues in the lines were not detected in Western blot analysis.					
33547931	9	71	located	detected	1349:1356	arg1	line					1365:1368	the line	1361:1368	the line	1361:1368	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	9	71	located	detected	1349:1356	arg2	residues					1331:1338	α1,3-fucose and β1,2-xylose residues	1303:1338	α1,3-fucose and β1,2-xylose residues	1303:1338	Although α1,3-fucose and β1,2-xylose residues were not detected in the line, other plant-specific residues of β1,3-galactose and α1,4-fucose were detected.					
33547931	5	72	theme	plant-specific	874:887	arg1	genes					898:902	the plant-specific N-glycan genes	870:902	the plant-specific N-glycan genes of rice (Oryza sativa)	870:925	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	5	72	theme	plant-specific	874:887	arg1	Oryza					913:917	Oryza	913:917	Oryza	913:917	As an effort to use plants as a platform to produce biopharmaceuticals, the plant-specific N-glycan genes of rice (Oryza sativa), β1,2-xylT (OsXylT) and α1,3-FucT (OsFucT), were knocked out using multiplex CRISPR/Cas9 technology.					
33547931	4	73	from	structure	677:685	arg1	glycoproteins					690:702	glycoproteins	690:702	glycoproteins	690:702	In plant-specific manner, β1,2-xylose and α1,3-fucose residues are transferred to N-glycan core structure on glycoproteins by β1,2-xylosyltransferase (β1,2-XylT) and α1,3-fucosyltransferase (α1,3-FucT), respectively.					
33289761	0	0	theme	efficient	93:101	arg1	capture					103:109	efficient capture	93:109	efficient capture of N-linked glycans	93:129	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	4	1	theme	capture	932:938	arg1	performance					940:950	the capture performance	928:950	the capture performance	928:950	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	3	2	theme	5	719:719	arg1	μL					721:722	μL	721:722	μL	721:722	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	4	3	from	nanoparticles	838:850	arg1	FeNi-G/PC-800					888:900	FeNi-G/PC-800	888:900	FeNi-G/PC-800	888:900	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	3	4	theme	μL	721:722	arg1	serum					730:734	5 μL human serum	719:734	5 μL human serum	719:734	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	4	5	theme	integrated	822:831	arg1	nanoparticles					838:850	synergistically integrated FeNi nanoparticles	806:850	synergistically integrated FeNi nanoparticles	806:850	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	4	6	dep	nanoparticles	838:850	arg1	the					802:804	the	802:804	the	802:804	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	3	7	from	profiling	683:691	arg1	serum					730:734	5 μL human serum	719:734	5 μL human serum	719:734	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	3	8	from	serum	648:652	arg1	glycans					599:605	N-linked glycans	590:605	N-linked glycans from standard bio-samples and real human serum	590:652	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	3	9	theme	N-linked	506:513	arg1	glycans					515:521	N-linked glycans	506:521	N-linked glycans	506:521	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	0	10	theme	glycans	123:129	arg1	capture					103:109	efficient capture	93:109	efficient capture of N-linked glycans	93:129	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	4	11	theme	Structure-property	737:754	arg1	studies					769:775	Structure-property relationship studies	737:775	Structure-property relationship studies	737:775	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	4	12	theme	key	916:918	arg1	role					920:923	a key role	914:923	a key role	914:923	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	3	13	theme	capture	495:501	arg1	Studies					484:490	Studies	484:490	Studies of capture of N-linked glycans	484:521	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	3	14	theme	remarkable	557:566	arg1	performance					568:578	a remarkable performance	555:578	a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum	555:652	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	0	15	theme	N-linked	114:121	arg1	glycans					123:129	N-linked glycans	114:129	N-linked glycans	114:129	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	2	16	theme	carbon	290:295	arg1	composites					297:306	ferronickel graphene-based porous carbon composites	256:306	ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature)	256:351	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	2	17	theme	synergistic	404:414	arg1	integration					416:426	the synergistic integration	400:426	the synergistic integration of magnetic separation, porosity and polar interaction	400:481	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	2	18	theme	interaction	471:481	arg1	integration					416:426	the synergistic integration	400:426	the synergistic integration of magnetic separation, porosity and polar interaction	400:481	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	2	19	theme	carbonization	326:338	arg1	temperature					340:350	T = carbonization temperature	322:350	T = carbonization temperature	322:350	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	2	19	theme	carbonization	326:338	arg1	FeNi-G/PC-T					309:319	FeNi-G/PC-T	309:319	FeNi-G/PC-T	309:319	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	0	20	theme	Synergistic	0:10	arg1	integration					12:22	Synergistic integration	0:22	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.	0:130	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	4	21	theme	relationship	756:767	arg1	studies					769:775	Structure-property relationship studies	737:775	Structure-property relationship studies	737:775	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	2	22	theme	=	324:324	arg1	temperature					340:350	T = carbonization temperature	322:350	T = carbonization temperature	322:350	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	2	22	theme	=	324:324	arg1	FeNi-G/PC-T					309:319	FeNi-G/PC-T	309:319	FeNi-G/PC-T	309:319	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	3	23	from	bio-samples	621:631	arg1	glycans					599:605	N-linked glycans	590:605	N-linked glycans from standard bio-samples and real human serum	590:652	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	0	24	theme	FeNi	27:30	arg1	nanoparticles					41:53	FeNi magnetic nanoparticles	27:53	FeNi magnetic nanoparticles	27:53	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	3	25	theme	N-linked	590:597	arg1	glycans					599:605	N-linked glycans	590:605	N-linked glycans from standard bio-samples and real human serum	590:652	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	1	26	theme	N-glycans	206:214	arg1	capture					195:201	the capture	191:201	the capture of N-glycans from complex bio-samples	191:239	Developing functional porous carbon is greatly desired for the capture of N-glycans from complex bio-samples.					
33289761	2	27	theme	porosity	452:459	arg1	integration					416:426	the synergistic integration	400:426	the synergistic integration of magnetic separation, porosity and polar interaction	400:481	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	2	28	theme	polar	465:469	arg1	interaction					471:481	polar interaction	465:481	polar interaction	465:481	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	2	29	theme	separation	440:449	arg1	integration					416:426	the synergistic integration	400:426	the synergistic integration of magnetic separation, porosity and polar interaction	400:481	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	0	30	theme	nanoparticles	41:53	arg1	integration					12:22	Synergistic integration	0:22	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.	0:130	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	2	31	theme	magnetic	431:438	arg1	separation					440:449	magnetic separation	431:449	magnetic separation	431:449	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	3	32	theme	real	637:640	arg1	serum					648:652	real human serum	637:652	real human serum	637:652	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	1	33	theme	complex	221:227	arg1	bio-samples					229:239	complex bio-samples	221:239	complex bio-samples	221:239	Developing functional porous carbon is greatly desired for the capture of N-glycans from complex bio-samples.					
33289761	0	34	theme	magnetic	32:39	arg1	nanoparticles					41:53	FeNi magnetic nanoparticles	27:53	FeNi magnetic nanoparticles	27:53	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	3	35	theme	human	642:646	arg1	serum					648:652	real human serum	637:652	real human serum	637:652	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	0	36	theme	graphene-based	60:73	arg1	carbon					82:87	graphene-based porous carbon	60:87	graphene-based porous carbon for efficient capture of N-linked glycans	60:129	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	3	37	theme	successful	672:681	arg1	profiling					683:691	the successful profiling	668:691	the successful profiling of 48 N-linked glycans in 5 μL human serum	668:734	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	1	38	from	bio-samples	229:239	arg1	capture					195:201	the capture	191:201	the capture of N-glycans from complex bio-samples	191:239	Developing functional porous carbon is greatly desired for the capture of N-glycans from complex bio-samples.					
33289761	3	39	theme	glycans	515:521	arg1	capture					495:501	capture	495:501	capture of N-linked glycans	495:521	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	3	40	link	N-linked	506:513	arg1	glycans					515:521	N-linked glycans	506:521	N-linked glycans	506:521	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	3	41	theme	glycans	708:714	arg1	profiling					683:691	the successful profiling	668:691	the successful profiling of 48 N-linked glycans in 5 μL human serum	668:734	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	0	42	with	integration	12:22	arg1	carbon					82:87	graphene-based porous carbon	60:87	graphene-based porous carbon for efficient capture of N-linked glycans	60:129	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	1	43	theme	Developing	132:141	arg1	carbon					161:166	Developing functional porous carbon	132:166	Developing functional porous carbon	132:166	Developing functional porous carbon is greatly desired for the capture of N-glycans from complex bio-samples.					
33289761	4	44	theme	graphene-based	856:869	arg1	carbon					878:883	graphene-based porous carbon	856:883	graphene-based porous carbon	856:883	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	2	45	theme	porous	283:288	arg1	composites					297:306	ferronickel graphene-based porous carbon composites	256:306	ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature)	256:351	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	1	46	theme	functional	143:152	arg1	carbon					161:166	Developing functional porous carbon	132:166	Developing functional porous carbon	132:166	Developing functional porous carbon is greatly desired for the capture of N-glycans from complex bio-samples.					
33289761	3	47	link	N-linked	699:706	arg1	glycans					708:714	48 N-linked glycans	696:714	48 N-linked glycans	696:714	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	4	48	theme	FeNi	833:836	arg1	nanoparticles					838:850	synergistically integrated FeNi nanoparticles	806:850	synergistically integrated FeNi nanoparticles	806:850	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	2	49	theme	graphene-based	268:281	arg1	composites					297:306	ferronickel graphene-based porous carbon composites	256:306	ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature)	256:351	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	4	50	from	carbon	878:883	arg1	FeNi-G/PC-800					888:900	FeNi-G/PC-800	888:900	FeNi-G/PC-800	888:900	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	1	51	theme	porous	154:159	arg1	carbon					161:166	Developing functional porous carbon	132:166	Developing functional porous carbon	132:166	Developing functional porous carbon is greatly desired for the capture of N-glycans from complex bio-samples.					
33289761	3	52	link	N-linked	590:597	arg1	glycans					599:605	N-linked glycans	590:605	N-linked glycans from standard bio-samples and real human serum	590:652	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	3	53	theme	N-linked	699:706	arg1	glycans					708:714	48 N-linked glycans	696:714	48 N-linked glycans	696:714	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	2	54	theme	ferronickel	256:266	arg1	composites					297:306	ferronickel graphene-based porous carbon composites	256:306	ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature)	256:351	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	0	55	link	N-linked	114:121	arg1	glycans					123:129	N-linked glycans	114:129	N-linked glycans	114:129	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	2	56	dep	composites	297:306	arg1	temperature					340:350	T = carbonization temperature	322:350	T = carbonization temperature	322:350	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	2	56	dep	composites	297:306	arg1	FeNi-G/PC-T					309:319	FeNi-G/PC-T	309:319	FeNi-G/PC-T	309:319	In this work, ferronickel graphene-based porous carbon composites (FeNi-G/PC-T, T = carbonization temperature) are facilely prepared and are characterized by the synergistic integration of magnetic separation, porosity and polar interaction.					
33289761	0	57	theme	porous	75:80	arg1	carbon					82:87	graphene-based porous carbon	60:87	graphene-based porous carbon for efficient capture of N-linked glycans	60:129	Synergistic integration of FeNi magnetic nanoparticles with graphene-based porous carbon for efficient capture of N-linked glycans.					
33289761	3	58	theme	human	724:728	arg1	serum					730:734	5 μL human serum	719:734	5 μL human serum	719:734	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33289761	4	59	theme	porous	871:876	arg1	carbon					878:883	graphene-based porous carbon	856:883	graphene-based porous carbon	856:883	Structure-property relationship studies further demonstrate that the synergistically integrated FeNi nanoparticles and graphene-based porous carbon in FeNi-G/PC-800 should play a key role in the capture performance.					
33289761	3	60	theme	standard	612:619	arg1	bio-samples					621:631	standard bio-samples	612:631	standard bio-samples	612:631	Studies of capture of N-linked glycans reveal that FeNi-G/PC-800 shows a remarkable performance to enrich N-linked glycans from standard bio-samples and real human serum, resulting in the successful profiling of 48 N-linked glycans in 5 μL human serum.					
33035859	3	0	dep	identified	550:559	arg1	whereas					591:597	whereas	591:597	whereas	591:597	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	2	1	theme	heating	470:476	arg1	conditions					483:492	water heating (WH) or microwave heating (MH) conditions	438:492	water heating (WH) or microwave heating (MH) conditions	438:492	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	3	2	located	detected	641:648	arg2	sites					630:634	6O-linked, 2N-linked glycation sites	599:634	6O-linked, 2N-linked glycation sites	599:634	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	3	2	located	detected	641:648	arg1	conjugates					668:677	the OVA-Dex-WH conjugates	653:677	the OVA-Dex-WH conjugates	653:677	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	5	3	theme	high	895:898	arg1	reactivity					910:919	high glycation reactivity	895:919	high glycation reactivity	895:919	In addition, β-sheet and β-turn structures showed high glycation reactivity.					
33035859	1	4	theme	important	168:176	arg1	relationship					178:189	An important relationship	165:189	An important relationship	165:189	An important relationship exists between the changes in glycation sites and structure of proteins.					
33035859	6	5	theme	glycation	1012:1020	arg1	sites					1022:1026	the glycation sites	1008:1026	the glycation sites that were mainly found in hydrophilic amino acids	1008:1076	The increased surface hydrophobicity of OVA-Dex conjugates was possibly attributed to the glycation sites that were mainly found in hydrophilic amino acids.					
33035859	0	6	theme	capillary	94:102	arg1	chromatography					111:124	capillary liquid chromatography	94:124	capillary liquid chromatography coupled with tandem mass spectrometry	94:162	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites identification by capillary liquid chromatography coupled with tandem mass spectrometry.					
33035859	4	7	theme	different	780:788	arg1	reactivity					800:809	different glycation reactivity	780:809	different glycation reactivity	780:809	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	7	8	theme	glycation	1125:1133	arg1	research					1145:1152	the glycation mechanism research	1121:1152	the glycation mechanism research of OVA and Dex	1121:1167	Our study provides useful information for the glycation mechanism research of OVA and Dex.					
33035859	2	9	theme	tandem	307:312	arg1	spectrometry					319:330	tandem mass spectrometry	307:330	tandem mass spectrometry	307:330	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	4	10	theme	OVA	751:753	arg1	structure					765:773	OVA molecular structure	751:773	OVA molecular structure	751:773	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	7	11	theme	Dex	1165:1167	arg1	research					1145:1152	the glycation mechanism research	1121:1152	the glycation mechanism research of OVA and Dex	1121:1167	Our study provides useful information for the glycation mechanism research of OVA and Dex.					
33035859	3	12	theme	glycation	529:537	arg1	sites					539:543	12O-linked glycation sites	518:543	12O-linked glycation sites	518:543	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	3	13	link	12O-linked	518:527	arg1	sites					539:543	12O-linked glycation sites	518:543	12O-linked glycation sites	518:543	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	6	14	theme	amino	1066:1070	arg1	acids					1072:1076	hydrophilic amino acids	1054:1076	hydrophilic amino acids	1054:1076	The increased surface hydrophobicity of OVA-Dex conjugates was possibly attributed to the glycation sites that were mainly found in hydrophilic amino acids.					
33035859	2	15	theme	chromatography	288:301	arg1	combination					266:276	A combination	264:276	A combination of liquid chromatography and tandem mass spectrometry	264:330	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	3	16	link	6O-linked	599:607	arg1	sites					630:634	6O-linked, 2N-linked glycation sites	599:634	6O-linked, 2N-linked glycation sites	599:634	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	2	17	theme	dextran	406:412	arg1	conjugation					420:430	dextran (Dex) conjugation	406:430	dextran (Dex) conjugation	406:430	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	0	18	theme	liquid	104:109	arg1	chromatography					111:124	capillary liquid chromatography	94:124	capillary liquid chromatography coupled with tandem mass spectrometry	94:162	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites identification by capillary liquid chromatography coupled with tandem mass spectrometry.					
33035859	5	19	theme	glycation	900:908	arg1	reactivity					910:919	high glycation reactivity	895:919	high glycation reactivity	895:919	In addition, β-sheet and β-turn structures showed high glycation reactivity.					
33035859	0	20	theme	ovalbumin	36:44	arg1	reaction					24:31	glycation reaction	14:31	glycation reaction of ovalbumin with dextran	14:57	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites identification by capillary liquid chromatography coupled with tandem mass spectrometry.					
33035859	2	21	theme	liquid	281:286	arg1	chromatography					288:301	liquid chromatography	281:301	liquid chromatography	281:301	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	7	22	theme	useful	1098:1103	arg1	information					1105:1115	useful information	1098:1115	useful information for the glycation mechanism research of OVA and Dex	1098:1167	Our study provides useful information for the glycation mechanism research of OVA and Dex.					
33035859	4	23	from	positions	738:746	arg1	acids					719:723	the amino acids	709:723	the amino acids at different positions in OVA molecular structure	709:773	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	4	24	theme	different	728:736	arg1	positions					738:746	different positions	728:746	different positions	728:746	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	2	25	theme	heating	444:450	arg1	conditions					483:492	water heating (WH) or microwave heating (MH) conditions	438:492	water heating (WH) or microwave heating (MH) conditions	438:492	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	6	26	located	found	1045:1049	arg1	acids					1072:1076	hydrophilic amino acids	1054:1076	hydrophilic amino acids	1054:1076	The increased surface hydrophobicity of OVA-Dex conjugates was possibly attributed to the glycation sites that were mainly found in hydrophilic amino acids.					
33035859	6	26	located	found	1045:1049	arg2	sites					1022:1026	the glycation sites	1008:1026	the glycation sites that were mainly found in hydrophilic amino acids	1008:1076	The increased surface hydrophobicity of OVA-Dex conjugates was possibly attributed to the glycation sites that were mainly found in hydrophilic amino acids.					
33035859	4	27	theme	molecular	755:763	arg1	structure					765:773	OVA molecular structure	751:773	OVA molecular structure	751:773	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	0	28	theme	reaction	24:31	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites	0:74	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites identification by capillary liquid chromatography coupled with tandem mass spectrometry.					
33035859	7	29	theme	mechanism	1135:1143	arg1	research					1145:1152	the glycation mechanism research	1121:1152	the glycation mechanism research of OVA and Dex	1121:1167	Our study provides useful information for the glycation mechanism research of OVA and Dex.					
33035859	2	30	from	ovalbumin	384:392	arg1	information					367:377	the glycation information	353:377	the glycation information from ovalbumin (OVA)	353:398	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	2	31	theme	spectrometry	319:330	arg1	combination					266:276	A combination	264:276	A combination of liquid chromatography and tandem mass spectrometry	264:330	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	0	32	theme	glycation	14:22	arg1	reaction					24:31	glycation reaction	14:31	glycation reaction of ovalbumin with dextran	14:57	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites identification by capillary liquid chromatography coupled with tandem mass spectrometry.					
33035859	2	33	theme	mass	314:317	arg1	spectrometry					319:330	tandem mass spectrometry	307:330	tandem mass spectrometry	307:330	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	3	34	dep	6O-linked	599:607	arg1	2N-linked					610:618	2N-linked	610:618	2N-linked	610:618	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	2	35	theme	microwave	460:468	arg1	MH					479:480	MH	479:480	MH	479:480	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	2	35	theme	microwave	460:468	arg1	heating					470:476	microwave heating	460:476	microwave heating (MH)	460:481	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	6	36	theme	conjugates	970:979	arg1	hydrophobicity					944:957	The increased surface hydrophobicity	922:957	The increased surface hydrophobicity of OVA-Dex conjugates	922:979	The increased surface hydrophobicity of OVA-Dex conjugates was possibly attributed to the glycation sites that were mainly found in hydrophilic amino acids.					
33035859	0	37	theme	mass	146:149	arg1	spectrometry					151:162	tandem mass spectrometry	139:162	tandem mass spectrometry	139:162	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites identification by capillary liquid chromatography coupled with tandem mass spectrometry.					
33035859	6	38	theme	hydrophilic	1054:1064	arg1	acids					1072:1076	hydrophilic amino acids	1054:1076	hydrophilic amino acids	1054:1076	The increased surface hydrophobicity of OVA-Dex conjugates was possibly attributed to the glycation sites that were mainly found in hydrophilic amino acids.					
33035859	1	39	theme	glycation	221:229	arg1	sites					231:235	glycation sites	221:235	glycation sites	221:235	An important relationship exists between the changes in glycation sites and structure of proteins.					
33035859	6	40	theme	OVA-Dex	962:968	arg1	conjugates					970:979	OVA-Dex conjugates	962:979	OVA-Dex conjugates	962:979	The increased surface hydrophobicity of OVA-Dex conjugates was possibly attributed to the glycation sites that were mainly found in hydrophilic amino acids.					
33035859	0	41	theme	tandem	139:144	arg1	spectrometry					151:162	tandem mass spectrometry	139:162	tandem mass spectrometry	139:162	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites identification by capillary liquid chromatography coupled with tandem mass spectrometry.					
33035859	3	42	theme	glycation	620:628	arg1	sites					630:634	6O-linked, 2N-linked glycation sites	599:634	6O-linked, 2N-linked glycation sites	599:634	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	2	43	theme	water	438:442	arg1	heating					444:450	water heating	438:450	water heating (WH)	438:455	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	2	43	theme	water	438:442	arg1	WH					453:454	WH	453:454	WH	453:454	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	0	44	with	reaction	24:31	arg1	dextran					51:57	dextran	51:57	dextran	51:57	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites identification by capillary liquid chromatography coupled with tandem mass spectrometry.					
33035859	6	45	theme	surface	936:942	arg1	hydrophobicity					944:957	The increased surface hydrophobicity	922:957	The increased surface hydrophobicity of OVA-Dex conjugates	922:979	The increased surface hydrophobicity of OVA-Dex conjugates was possibly attributed to the glycation sites that were mainly found in hydrophilic amino acids.					
33035859	7	46	theme	OVA	1157:1159	arg1	research					1145:1152	the glycation mechanism research	1121:1152	the glycation mechanism research of OVA and Dex	1121:1167	Our study provides useful information for the glycation mechanism research of OVA and Dex.					
33035859	4	47	contain	have	775:778	arg2	reactivity					800:809	different glycation reactivity	780:809	different glycation reactivity	780:809	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	4	47	contain	have	775:778	arg1	acids					719:723	the amino acids	709:723	the amino acids at different positions in OVA molecular structure	709:773	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	4	48	theme	induction	826:834	arg1	systems					836:842	WH induction systems	823:842	WH induction systems	823:842	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	2	49	theme	glycation	357:365	arg1	information					367:377	the glycation information	353:377	the glycation information from ovalbumin (OVA)	353:398	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	3	50	theme	OVA-Dex-WH	657:666	arg1	conjugates					668:677	the OVA-Dex-WH conjugates	653:677	the OVA-Dex-WH conjugates	653:677	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	4	51	theme	WH	823:824	arg1	systems					836:842	WH induction systems	823:842	WH induction systems	823:842	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	1	52	from	changes	210:216	arg1	sites					231:235	glycation sites	221:235	glycation sites	221:235	An important relationship exists between the changes in glycation sites and structure of proteins.					
33035859	4	53	from	acids	719:723	arg1	structure					765:773	OVA molecular structure	751:773	OVA molecular structure	751:773	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	3	54	theme	6O-linked	599:607	arg1	sites					630:634	6O-linked, 2N-linked glycation sites	599:634	6O-linked, 2N-linked glycation sites	599:634	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	4	55	theme	glycation	790:798	arg1	reactivity					800:809	different glycation reactivity	780:809	different glycation reactivity	780:809	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	1	56	from	structure	241:249	arg1	sites					231:235	glycation sites	221:235	glycation sites	221:235	An important relationship exists between the changes in glycation sites and structure of proteins.					
33035859	3	57	theme	12O-linked	518:527	arg1	sites					539:543	12O-linked glycation sites	518:543	12O-linked glycation sites	518:543	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	4	58	theme	amino	713:717	arg1	acids					719:723	the amino acids	709:723	the amino acids at different positions in OVA molecular structure	709:773	These findings indicate that the amino acids at different positions in OVA molecular structure have different glycation reactivity under MH or WH induction systems.					
33035859	0	59	theme	Glycation	60:68	arg1	sites					70:74	Glycation sites	60:74	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites	0:74	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites identification by capillary liquid chromatography coupled with tandem mass spectrometry.					
33035859	5	60	dep	β-sheet	858:864	arg1	structures					877:886	structures	877:886	structures	877:886	In addition, β-sheet and β-turn structures showed high glycation reactivity.					
33035859	2	61	used	used	336:339	arg2	combination					266:276	A combination	264:276	A combination of liquid chromatography and tandem mass spectrometry	264:330	A combination of liquid chromatography and tandem mass spectrometry was used to identify the glycation information from ovalbumin (OVA) after dextran (Dex) conjugation under water heating (WH) or microwave heating (MH) conditions.					
33035859	6	62	theme	increased	926:934	arg1	hydrophobicity					944:957	The increased surface hydrophobicity	922:957	The increased surface hydrophobicity of OVA-Dex conjugates	922:979	The increased surface hydrophobicity of OVA-Dex conjugates was possibly attributed to the glycation sites that were mainly found in hydrophilic amino acids.					
33035859	0	63	dep	Evaluation	0:9	arg1	sites					70:74	Glycation sites	60:74	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites	0:74	Evaluation of glycation reaction of ovalbumin with dextran: Glycation sites identification by capillary liquid chromatography coupled with tandem mass spectrometry.					
33035859	3	64	theme	Dex	501:503	arg1	conjugation					505:515	Dex conjugation	501:515	Dex conjugation	501:515	After Dex conjugation, 12O-linked glycation sites were identified in the OVA-Dex-MH conjugates, whereas 6O-linked, 2N-linked glycation sites were detected in the OVA-Dex-WH conjugates.					
33035859	1	65	theme	proteins	254:261	arg1	structure					241:249	structure	241:249	structure of proteins	241:261	An important relationship exists between the changes in glycation sites and structure of proteins.					
33035859	1	65	theme	proteins	254:261	arg1	changes					210:216	the changes	206:216	the changes in glycation sites	206:235	An important relationship exists between the changes in glycation sites and structure of proteins.					
32623356	14	0	theme	HMGB1	2174:2178	arg1	modifications					2157:2169	the many post-translational modifications	2129:2169	the many post-translational modifications of HMGB1	2129:2178	For the first time, it is shown (at least in silico) that N-glycosylation, one of the many post-translational modifications of HMGB1, can affect drug binding.					
32623356	10	1	theme	direct	1546:1551	arg1	effects					1566:1572	both direct and indirect effects	1541:1572	both direct and indirect effects	1541:1572	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32623356	13	2	theme	carbohydrate-carbohydrate	1944:1968	arg1	interaction					1970:1980	a carbohydrate-carbohydrate interaction	1942:1980	a carbohydrate-carbohydrate interaction	1942:1980	The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.					
32623356	14	3	theme	modifications	2157:2169	arg1	modifications					2157:2169	the many post-translational modifications	2129:2169	the many post-translational modifications of HMGB1	2129:2178	For the first time, it is shown (at least in silico) that N-glycosylation, one of the many post-translational modifications of HMGB1, can affect drug binding.					
32623356	14	3	theme	modifications	2157:2169	arg1	one					2122:2124	one	2122:2124	one	2122:2124	For the first time, it is shown (at least in silico) that N-glycosylation, one of the many post-translational modifications of HMGB1, can affect drug binding.					
32623356	14	3	theme	modifications	2157:2169	arg1	N-glycosylation					2105:2119	N-glycosylation	2105:2119	N-glycosylation	2105:2119	For the first time, it is shown (at least in silico) that N-glycosylation, one of the many post-translational modifications of HMGB1, can affect drug binding.					
32623356	14	4	theme	many	2133:2136	arg1	modifications					2157:2169	the many post-translational modifications	2129:2169	the many post-translational modifications of HMGB1	2129:2178	For the first time, it is shown (at least in silico) that N-glycosylation, one of the many post-translational modifications of HMGB1, can affect drug binding.					
32623356	1	5	theme	Box	154:156	arg1	protein					191:197	an abundant protein	179:197	an abundant protein with multiple functions in cells	179:230	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	1	5	theme	Box	154:156	arg1	HMGB1					169:173	HMGB1	169:173	HMGB1	169:173	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	1	5	theme	Box	154:156	arg1	protein					160:166	High Mobility Group Box 1 protein	134:166	High Mobility Group Box 1 protein (HMGB1)	134:174	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	4	6	theme	adjacent	588:595	arg1	boxes					601:605	the two adjacent HMG boxes	580:605	the two adjacent HMG boxes of the non-glycosylated protein	580:637	The drug is known to bind to each of the two adjacent HMG boxes of the non-glycosylated protein.					
32623356	11	7	theme	HMG	1634:1636	arg1	box					1638:1640	the HMG box	1630:1640	the HMG box	1630:1640	The calculations indicate that binding of 18α/β-GLR to the HMG box is generally reduced when the protein is N-glycosylated vs. the non-glycosylated protein.					
32623356	0	8	with	interaction	75:85	arg1	glycyrrhizin					92:103	glycyrrhizin	92:103	glycyrrhizin: A molecular modeling study	92:131	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	3	9	theme	liver	518:522	arg1	diseases					533:540	diverse liver and skin diseases	510:540	diverse liver and skin diseases	510:540	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	5	10	theme	nucleocytoplasmic	771:787	arg1	transport					789:797	the nucleocytoplasmic transport	767:797	the nucleocytoplasmic transport of the protein	767:812	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	12	11	theme	GLR	1826:1828	arg1	binding					1815:1821	the binding	1811:1821	the binding of GLR to box-B	1811:1837	In particular, the N-glycans in an extended configuration significantly weaken the binding of GLR to box-B.					
32623356	8	12	theme	Sialylated	1082:1091	arg1	N-glycans					1106:1114	Sialylated bi-antennary N-glycans	1082:1114	Sialylated bi-antennary N-glycans	1082:1114	Sialylated bi-antennary N-glycans were introduced on the protein and exposed in a folded or an extended conformation for the drug binding study.					
32623356	6	13	theme	drug	850:853	arg1	binding					855:861	drug binding	850:861	drug binding	850:861	But the impact of the N-glycans on drug binding is unknown.					
32623356	11	14	gly	non-glycosylated	1706:1721	arg1	protein					1723:1729	the non-glycosylated protein	1702:1729	the non-glycosylated protein	1702:1729	The calculations indicate that binding of 18α/β-GLR to the HMG box is generally reduced when the protein is N-glycosylated vs. the non-glycosylated protein.					
32623356	14	15	dep	shown	2073:2077	arg1	silico					2092:2097	silico	2092:2097	silico	2092:2097	For the first time, it is shown (at least in silico) that N-glycosylation, one of the many post-translational modifications of HMGB1, can affect drug binding.					
32623356	7	16	from	effect	905:910	arg1	interaction					951:961	its interaction	947:961	its interaction with GLR	947:970	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	0	17	theme	molecular	108:116	arg1	study					127:131	A molecular modeling study	106:131	glycyrrhizin: A molecular modeling study	92:131	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	5	18	from	cells	643:647	arg1	N-glycosylated					659:672	N-glycosylated	659:672	N-glycosylated	659:672	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	7	19	theme	Human	1043:1047	arg1	2YRQ					1075:1078	PDB code 2YRQ	1066:1078	PDB code 2YRQ	1066:1078	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	7	19	theme	Human	1043:1047	arg1	structure					1055:1063	a Human HMGB1 structure	1041:1063	a Human HMGB1 structure (PDB code 2YRQ)	1041:1079	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	6	20	theme	N-glycans	837:845	arg1	unknown					866:872	unknown	866:872	unknown	866:872	But the impact of the N-glycans on drug binding is unknown.					
32623356	6	20	theme	N-glycans	837:845	arg1	impact					823:828	the impact	819:828	the impact of the N-glycans on drug binding	819:861	But the impact of the N-glycans on drug binding is unknown.					
32623356	4	21	theme	protein	631:637	arg1	boxes					601:605	the two adjacent HMG boxes	580:605	the two adjacent HMG boxes of the non-glycosylated protein	580:637	The drug is known to bind to each of the two adjacent HMG boxes of the non-glycosylated protein.					
32623356	10	22	theme	binding	1459:1465	arg1	sites					1467:1471	the drug binding sites	1450:1471	the drug binding sites on boxes A and B	1450:1488	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32623356	7	23	theme	HMGB1	938:942	arg1	N-glycosylation					919:933	the N-glycosylation	915:933	the N-glycosylation of HMGB1	915:942	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	3	24	theme	skin	528:531	arg1	diseases					533:540	diverse liver and skin diseases	510:540	diverse liver and skin diseases	510:540	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	9	25	theme	interaction	1350:1360	arg1	conformations					1312:1324	conformations	1312:1324	conformations	1312:1324	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	9	25	theme	interaction	1350:1360	arg1	energy					1340:1345	potential energy	1330:1345	potential energy	1330:1345	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	7	26	gly	N-glycosylation	919:933	arg1	HMGB1					938:942	HMGB1	938:942	HMGB1	938:942	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	7	27	theme	N-glycosylation	919:933	arg1	effect					905:910	the effect	901:910	the effect of the N-glycosylation of HMGB1 on its interaction with GLR	901:970	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	5	28	from	N-glycosylated	659:672	arg1	cells					643:647	cells	643:647	cells	643:647	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	1	29	theme	molecular	281:289	arg1	molecule					299:306	damage-associated molecular pattern molecule	263:306	damage-associated molecular pattern molecule	263:306	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	0	30	theme	High	19:22	arg1	HMGB1					54:58	HMGB1	54:58	HMGB1	54:58	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	0	30	theme	High	19:22	arg1	protein					45:51	High Mobility Group Box 1 protein	19:51	High Mobility Group Box 1 protein (HMGB1)	19:59	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	5	31	theme	asparagine	683:692	arg1	residues					694:701	three asparagine residues	677:701	three asparagine residues located in boxes A and B	677:726	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	5	32	from	residues	694:701	arg1	N-glycosylated					659:672	N-glycosylated	659:672	N-glycosylated	659:672	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	13	33	located	observed	1987:1994	arg2	contact					1915:1921	a direct contact	1906:1921	a direct contact	1906:1921	The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.					
32623356	13	33	located	observed	1987:1994	arg1	case					1901:1904	one case	1897:1904	one case	1897:1904	The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.					
32623356	1	34	theme	Mobility	139:146	arg1	protein					191:197	an abundant protein	179:197	an abundant protein with multiple functions in cells	179:230	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	1	34	theme	Mobility	139:146	arg1	HMGB1					169:173	HMGB1	169:173	HMGB1	169:173	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	1	34	theme	Mobility	139:146	arg1	protein					160:166	High Mobility Group Box 1 protein	134:166	High Mobility Group Box 1 protein (HMGB1)	134:174	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	8	35	theme	binding	1212:1218	arg1	study					1220:1224	the drug binding study	1203:1224	the drug binding study	1203:1224	Sialylated bi-antennary N-glycans were introduced on the protein and exposed in a folded or an extended conformation for the drug binding study.					
32623356	9	36	theme	complexes	1397:1405	arg1	conformations					1312:1324	conformations	1312:1324	conformations	1312:1324	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	9	36	theme	complexes	1397:1405	arg1	energy					1340:1345	potential energy	1330:1345	potential energy	1330:1345	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	13	37	with	contact	1915:1921	arg1	drug					1932:1935	the drug	1928:1935	the drug	1928:1935	The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.					
32623356	13	38	theme	glycosylated	2027:2038	arg1	HMGB1					2040:2044	glycosylated HMGB1	2027:2044	glycosylated HMGB1	2027:2044	The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.					
32623356	10	39	theme	drug-protein	1511:1522	arg1	interaction					1524:1534	the drug-protein interaction	1507:1534	the drug-protein interaction	1507:1534	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32623356	7	40	theme	HMGB1	1049:1053	arg1	2YRQ					1075:1078	PDB code 2YRQ	1066:1078	PDB code 2YRQ	1066:1078	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	7	40	theme	HMGB1	1049:1053	arg1	structure					1055:1063	a Human HMGB1 structure	1041:1063	a Human HMGB1 structure (PDB code 2YRQ)	1041:1079	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	13	41	gly	glycosylated	2027:2038	arg1	HMGB1					2040:2044	glycosylated HMGB1	2027:2044	glycosylated HMGB1	2027:2044	The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.					
32623356	2	42	theme	inflammatory	365:376	arg1	diseases					378:385	inflammatory diseases	365:385	inflammatory diseases	365:385	It represents an attractive target for the treatment of inflammatory diseases and cancers.					
32623356	3	43	theme	natural	410:416	arg1	ligand					469:474	a well-characterized ligand	448:474	a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases	448:540	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	3	43	theme	natural	410:416	arg1	GLR					440:442	GLR	440:442	GLR	440:442	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	3	43	theme	natural	410:416	arg1	glycyrrhizin					426:437	The plant natural product glycyrrhizin	400:437	The plant natural product glycyrrhizin (GLR)	400:443	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	0	44	theme	Group	33:37	arg1	HMGB1					54:58	HMGB1	54:58	HMGB1	54:58	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	0	44	theme	Group	33:37	arg1	protein					45:51	High Mobility Group Box 1 protein	19:51	High Mobility Group Box 1 protein (HMGB1)	19:59	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	1	45	theme	DNA	245:247	arg1	chaperone					249:257	a DNA chaperone	243:257	a DNA chaperone	243:257	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	5	46	theme	located	703:709	arg1	residues					694:701	three asparagine residues	677:701	three asparagine residues located in boxes A and B	677:726	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	7	47	theme	N-glycans	1028:1036	arg1	incorporation					1005:1017	incorporation	1005:1017	incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ)	1005:1079	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	7	48	dep	2YRQ	1075:1078	arg1	code					1070:1073	code	1070:1073	code	1070:1073	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	13	49	theme	N-glycans	1859:1867	arg1	indirect					1880:1887	indirect	1880:1887	indirect	1880:1887	The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.					
32623356	13	49	theme	N-glycans	1859:1867	arg1	effects					1844:1850	The effects	1840:1850	The effects of the N-glycans	1840:1867	The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.					
32623356	9	50	theme	drug	1246:1249	arg1	docking					1231:1237	The docking	1227:1237	The docking of the drug	1227:1249	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	1	51	with	protein	191:197	arg1	functions					213:221	multiple functions	204:221	multiple functions in cells	204:230	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	4	52	theme	HMG	597:599	arg1	boxes					601:605	the two adjacent HMG boxes	580:605	the two adjacent HMG boxes of the non-glycosylated protein	580:637	The drug is known to bind to each of the two adjacent HMG boxes of the non-glycosylated protein.					
32623356	9	53	dep	conformations	1312:1324	arg1	the					1308:1310	the	1308:1310	the	1308:1310	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	2	54	theme	attractive	326:335	arg1	target					337:342	an attractive target	323:342	an attractive target for the treatment of inflammatory diseases and cancers	323:397	It represents an attractive target for the treatment of inflammatory diseases and cancers.					
32623356	9	55	theme	GLR	1300:1302	arg1	18α-					1276:1279	18α-	1276:1279	18α-	1276:1279	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	9	55	theme	GLR	1300:1302	arg1	18β-epimers					1285:1295	18β-epimers	1285:1295	18β-epimers of GLR	1285:1302	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	6	56	from	impact	823:828	arg1	binding					855:861	drug binding	850:861	drug binding	850:861	But the impact of the N-glycans on drug binding is unknown.					
32623356	3	57	theme	well-characterized	450:467	arg1	ligand					469:474	a well-characterized ligand	448:474	a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases	448:540	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	3	57	theme	well-characterized	450:467	arg1	glycyrrhizin					426:437	The plant natural product glycyrrhizin	400:437	The plant natural product glycyrrhizin (GLR)	400:443	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	1	58	theme	multiple	204:211	arg1	functions					213:221	multiple functions	204:221	multiple functions in cells	204:230	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	5	59	from	boxes	714:718	arg1	located					703:709	located	703:709	located	703:709	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	10	60	dep	boxes	1476:1480	arg1	B					1488:1488	B	1488:1488	B	1488:1488	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32623356	10	60	dep	boxes	1476:1480	arg1	A					1482:1482	A	1482:1482	A	1482:1482	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32623356	10	60	dep	boxes	1476:1480	arg1	boxes					1476:1480	boxes A and B	1476:1488	boxes A and B	1476:1488	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32623356	3	61	theme	drug	491:494	arg1	ligand					469:474	a well-characterized ligand	448:474	a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases	448:540	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	3	61	theme	drug	491:494	arg1	glycyrrhizin					426:437	The plant natural product glycyrrhizin	400:437	The plant natural product glycyrrhizin (GLR)	400:443	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	10	62	from	sites	1467:1471	arg1	B					1488:1488	B	1488:1488	B	1488:1488	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32623356	10	62	from	sites	1467:1471	arg1	A					1482:1482	A	1482:1482	A	1482:1482	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32623356	10	62	from	sites	1467:1471	arg1	boxes					1476:1480	boxes A and B	1476:1488	boxes A and B	1476:1488	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32623356	7	63	theme	molecular	978:986	arg1	modelling					988:996	molecular modelling	978:996	molecular modelling	978:996	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	14	64	theme	post-translational	2138:2155	arg1	modifications					2157:2169	the many post-translational modifications	2129:2169	the many post-translational modifications of HMGB1	2129:2178	For the first time, it is shown (at least in silico) that N-glycosylation, one of the many post-translational modifications of HMGB1, can affect drug binding.					
32623356	1	65	theme	Group	148:152	arg1	protein					191:197	an abundant protein	179:197	an abundant protein with multiple functions in cells	179:230	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	1	65	theme	Group	148:152	arg1	HMGB1					169:173	HMGB1	169:173	HMGB1	169:173	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	1	65	theme	Group	148:152	arg1	protein					160:166	High Mobility Group Box 1 protein	134:166	High Mobility Group Box 1 protein (HMGB1)	134:174	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	5	66	dep	boxes	714:718	arg1	boxes					714:718	boxes A and B	714:726	boxes A and B	714:726	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	5	66	dep	boxes	714:718	arg1	B					726:726	B	726:726	B	726:726	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	5	66	dep	boxes	714:718	arg1	A					720:720	A	720:720	A	720:720	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	10	67	theme	drug	1454:1457	arg1	sites					1467:1471	the drug binding sites	1450:1471	the drug binding sites on boxes A and B	1450:1488	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32623356	3	68	theme	diverse	510:516	arg1	diseases					533:540	diverse liver and skin diseases	510:540	diverse liver and skin diseases	510:540	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	4	69	gly	non-glycosylated	614:629	arg1	protein					631:637	the non-glycosylated protein	610:637	the non-glycosylated protein	610:637	The drug is known to bind to each of the two adjacent HMG boxes of the non-glycosylated protein.					
32623356	5	70	theme	protein	806:812	arg1	transport					789:797	the nucleocytoplasmic transport	767:797	the nucleocytoplasmic transport of the protein	767:812	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	9	71	theme	different	1374:1382	arg1	complexes					1397:1405	the different drug-protein complexes	1370:1405	the different drug-protein complexes	1370:1405	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	0	72	theme	modeling	118:125	arg1	study					127:131	A molecular modeling study	106:131	glycyrrhizin: A molecular modeling study	92:131	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	9	73	theme	potential	1330:1338	arg1	energy					1340:1345	potential energy	1330:1345	potential energy	1330:1345	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	12	74	theme	extended	1767:1774	arg1	configuration					1776:1788	an extended configuration	1764:1788	an extended configuration	1764:1788	In particular, the N-glycans in an extended configuration significantly weaken the binding of GLR to box-B.					
32623356	8	75	theme	bi-antennary	1093:1104	arg1	N-glycans					1106:1114	Sialylated bi-antennary N-glycans	1082:1114	Sialylated bi-antennary N-glycans	1082:1114	Sialylated bi-antennary N-glycans were introduced on the protein and exposed in a folded or an extended conformation for the drug binding study.					
32623356	11	76	gly	N-glycosylated	1683:1696	arg1	N-glycosylated					1683:1696	N-glycosylated	1683:1696	N-glycosylated	1683:1696	The calculations indicate that binding of 18α/β-GLR to the HMG box is generally reduced when the protein is N-glycosylated vs. the non-glycosylated protein.					
32623356	11	76	gly	N-glycosylated	1683:1696	arg1	protein					1672:1678	the protein	1668:1678	the protein	1668:1678	The calculations indicate that binding of 18α/β-GLR to the HMG box is generally reduced when the protein is N-glycosylated vs. the non-glycosylated protein.					
32623356	4	77	theme	non-glycosylated	614:629	arg1	protein					631:637	the non-glycosylated protein	610:637	the non-glycosylated protein	610:637	The drug is known to bind to each of the two adjacent HMG boxes of the non-glycosylated protein.					
32623356	13	78	theme	direct	1908:1913	arg1	contact					1915:1921	a direct contact	1906:1921	a direct contact	1906:1921	The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.					
32623356	9	79	theme	drug-protein	1384:1395	arg1	complexes					1397:1405	the different drug-protein complexes	1370:1405	the different drug-protein complexes	1370:1405	The docking of the drug was performed using both 18α- and 18β-epimers of GLR and the conformations and potential energy of interaction (ΔE) of the different drug-protein complexes were compared.					
32623356	12	80	from	N-glycans	1751:1759	arg1	configuration					1776:1788	an extended configuration	1764:1788	an extended configuration	1764:1788	In particular, the N-glycans in an extended configuration significantly weaken the binding of GLR to box-B.					
32623356	8	81	theme	extended	1177:1184	arg1	conformation					1186:1197	an extended conformation	1174:1197	an extended conformation	1174:1197	Sialylated bi-antennary N-glycans were introduced on the protein and exposed in a folded or an extended conformation for the drug binding study.					
32623356	1	82	theme	pattern	291:297	arg1	molecule					299:306	damage-associated molecular pattern molecule	263:306	damage-associated molecular pattern molecule	263:306	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	1	83	theme	High	134:137	arg1	protein					191:197	an abundant protein	179:197	an abundant protein with multiple functions in cells	179:230	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	1	83	theme	High	134:137	arg1	HMGB1					169:173	HMGB1	169:173	HMGB1	169:173	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	1	83	theme	High	134:137	arg1	protein					160:166	High Mobility Group Box 1 protein	134:166	High Mobility Group Box 1 protein (HMGB1)	134:174	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	14	84	gly	N-glycosylation	2105:2119	arg1	drug					2192:2195	drug binding	2192:2203	drug binding	2192:2203	For the first time, it is shown (at least in silico) that N-glycosylation, one of the many post-translational modifications of HMGB1, can affect drug binding.					
32623356	11	85	theme	18α/β-GLR	1617:1625	arg1	binding					1606:1612	binding	1606:1612	binding of 18α/β-GLR to the HMG box	1606:1640	The calculations indicate that binding of 18α/β-GLR to the HMG box is generally reduced when the protein is N-glycosylated vs. the non-glycosylated protein.					
32623356	5	86	from	located	703:709	arg1	boxes					714:718	boxes A and B	714:726	boxes A and B	714:726	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	5	86	from	located	703:709	arg1	B					726:726	B	726:726	B	726:726	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	5	86	from	located	703:709	arg1	A					720:720	A	720:720	A	720:720	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	0	87	dep	glycyrrhizin	92:103	arg1	study					127:131	A molecular modeling study	106:131	glycyrrhizin: A molecular modeling study	92:131	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	8	88	theme	drug	1207:1210	arg1	study					1220:1224	the drug binding study	1203:1224	the drug binding study	1203:1224	Sialylated bi-antennary N-glycans were introduced on the protein and exposed in a folded or an extended conformation for the drug binding study.					
32623356	5	89	gly	N-glycosylated	659:672	arg1	HMGB1					650:654	HMGB1	650:654	HMGB1	650:654	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	5	89	gly	N-glycosylated	659:672	arg2	residues					694:701	three asparagine residues	677:701	three asparagine residues located in boxes A and B	677:726	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	5	89	gly	N-glycosylated	659:672	arg1	cells					643:647	cells	643:647	cells	643:647	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	5	89	gly	N-glycosylated	659:672	arg2	HMGB1					650:654	HMGB1	650:654	HMGB1	650:654	In cells, HMGB1 is N-glycosylated at three asparagine residues located in boxes A and B, and these N-glycans are essential for the nucleocytoplasmic transport of the protein.					
32623356	7	90	theme	PDB	1066:1068	arg1	2YRQ					1075:1078	PDB code 2YRQ	1066:1078	PDB code 2YRQ	1066:1078	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	7	90	theme	PDB	1066:1068	arg1	structure					1055:1063	a Human HMGB1 structure	1041:1063	a Human HMGB1 structure (PDB code 2YRQ)	1041:1079	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	3	91	theme	plant	404:408	arg1	ligand					469:474	a well-characterized ligand	448:474	a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases	448:540	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	3	91	theme	plant	404:408	arg1	GLR					440:442	GLR	440:442	GLR	440:442	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	3	91	theme	plant	404:408	arg1	glycyrrhizin					426:437	The plant natural product glycyrrhizin	400:437	The plant natural product glycyrrhizin (GLR)	400:443	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	11	92	theme	non-glycosylated	1706:1721	arg1	protein					1723:1729	the non-glycosylated protein	1702:1729	the non-glycosylated protein	1702:1729	The calculations indicate that binding of 18α/β-GLR to the HMG box is generally reduced when the protein is N-glycosylated vs. the non-glycosylated protein.					
32623356	0	93	theme	Mobility	24:31	arg1	HMGB1					54:58	HMGB1	54:58	HMGB1	54:58	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	0	93	theme	Mobility	24:31	arg1	protein					45:51	High Mobility Group Box 1 protein	19:51	High Mobility Group Box 1 protein (HMGB1)	19:59	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	13	94	theme	HMGB1	2040:2044	arg1	Box-B					2018:2022	Box-B	2018:2022	Box-B of glycosylated HMGB1	2018:2044	The effects of the N-glycans are mostly indirect, but in one case a direct contact with the drug, via a carbohydrate-carbohydrate interaction, was observed with 18β-GLR bound to Box-B of glycosylated HMGB1.					
32623356	2	95	theme	cancers	391:397	arg1	treatment					352:360	the treatment	348:360	the treatment of inflammatory diseases and cancers	348:397	It represents an attractive target for the treatment of inflammatory diseases and cancers.					
32623356	14	96	theme	first	2055:2059	arg1	time					2061:2064	the first time	2051:2064	the first time	2051:2064	For the first time, it is shown (at least in silico) that N-glycosylation, one of the many post-translational modifications of HMGB1, can affect drug binding.					
32623356	2	97	theme	diseases	378:385	arg1	treatment					352:360	the treatment	348:360	the treatment of inflammatory diseases and cancers	348:397	It represents an attractive target for the treatment of inflammatory diseases and cancers.					
32623356	0	98	theme	Box	39:41	arg1	HMGB1					54:58	HMGB1	54:58	HMGB1	54:58	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	0	98	theme	Box	39:41	arg1	protein					45:51	High Mobility Group Box 1 protein	19:51	High Mobility Group Box 1 protein (HMGB1)	19:59	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	0	99	gly	N-glycosylation	0:14	arg1	HMGB1					54:58	HMGB1	54:58	HMGB1	54:58	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	0	99	gly	N-glycosylation	0:14	arg1	protein					45:51	High Mobility Group Box 1 protein	19:51	High Mobility Group Box 1 protein (HMGB1)	19:59	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	7	100	with	interaction	951:961	arg1	GLR					968:970	GLR	968:970	GLR	968:970	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	3	101	theme	product	418:424	arg1	ligand					469:474	a well-characterized ligand	448:474	a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases	448:540	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	3	101	theme	product	418:424	arg1	GLR					440:442	GLR	440:442	GLR	440:442	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	3	101	theme	product	418:424	arg1	glycyrrhizin					426:437	The plant natural product glycyrrhizin	400:437	The plant natural product glycyrrhizin (GLR)	400:443	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	0	102	theme	protein	45:51	arg1	N-glycosylation					0:14	N-glycosylation	0:14	N-glycosylation of High Mobility Group Box 1 protein (HMGB1)	0:59	N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study.					
32623356	1	103	from	functions	213:221	arg1	cells					226:230	cells	226:230	cells	226:230	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	7	104	from	incorporation	1005:1017	arg1	2YRQ					1075:1078	PDB code 2YRQ	1066:1078	PDB code 2YRQ	1066:1078	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	7	104	from	incorporation	1005:1017	arg1	structure					1055:1063	a Human HMGB1 structure	1041:1063	a Human HMGB1 structure (PDB code 2YRQ)	1041:1079	Here we have investigated the effect of the N-glycosylation of HMGB1 on its interaction with GLR using molecular modelling, after incorporation of three N-glycans on a Human HMGB1 structure (PDB code 2YRQ).					
32623356	1	105	theme	damage-associated	263:279	arg1	molecule					299:306	damage-associated molecular pattern molecule	263:306	damage-associated molecular pattern molecule	263:306	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	14	106	theme	drug	2192:2195	arg1	binding					2197:2203	drug binding	2192:2203	drug binding	2192:2203	For the first time, it is shown (at least in silico) that N-glycosylation, one of the many post-translational modifications of HMGB1, can affect drug binding.					
32623356	3	107	theme	HMGB1	479:483	arg1	ligand					469:474	a well-characterized ligand	448:474	a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases	448:540	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	3	107	theme	HMGB1	479:483	arg1	glycyrrhizin					426:437	The plant natural product glycyrrhizin	400:437	The plant natural product glycyrrhizin (GLR)	400:443	The plant natural product glycyrrhizin (GLR) is a well-characterized ligand of HMGB1 and a drug used to treat diverse liver and skin diseases.					
32623356	1	108	theme	abundant	182:189	arg1	protein					191:197	an abundant protein	179:197	an abundant protein with multiple functions in cells	179:230	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	1	108	theme	abundant	182:189	arg1	protein					160:166	High Mobility Group Box 1 protein	134:166	High Mobility Group Box 1 protein (HMGB1)	134:174	High Mobility Group Box 1 protein (HMGB1) is an abundant protein with multiple functions in cells, acting as a DNA chaperone and damage-associated molecular pattern molecule.					
32623356	10	109	theme	indirect	1557:1564	arg1	effects					1566:1572	both direct and indirect effects	1541:1572	both direct and indirect effects	1541:1572	The N-glycans do not shield the drug binding sites on boxes A and B but can modulate the drug-protein interaction, via both direct and indirect effects.					
32598367	7	0	theme	cancer	1310:1315	arg1	progression					1317:1327	cancer progression	1310:1327	cancer progression	1310:1327	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	9	1	theme	glycan	1888:1893	arg1	levels					1895:1900	glycan levels	1888:1900	glycan levels in similar ways	1888:1916	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	3	2	theme	tissue	441:446	arg1	samples					448:454	10 healthy tissue samples	430:454	10 healthy tissue samples	430:454	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	8	3	theme	significant	1443:1453	arg1	differences					1455:1465	no significant differences	1440:1465	no significant differences in the levels of any of the glycans analyzed	1440:1510	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	2	4	theme	important	248:256	arg1	functions					258:266	many important functions	243:266	many important functions relevant to cancer	243:285	Glycans, the sugar moieties of glycoconjugates, are involved in many important functions relevant to cancer and can be of value as biomarkers.					
32598367	5	5	theme	compositions	745:756	arg1	levels					707:712	The levels	703:712	The levels of neutral and acidic N-glycan compositions and glycan classes	703:775	The levels of neutral and acidic N-glycan compositions and glycan classes were analyzed in a total of ten different groups.					
32598367	6	6	theme	N-glycan	1015:1022	arg1	H3N5					1024:1027	the neutral N-glycan H3N5	1003:1027	the neutral N-glycan H3N5	1003:1027	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	8	7	theme	healthy	1669:1675	arg1	colon					1692:1696	the healthy right and left colon	1665:1696	the healthy right and left colon	1665:1696	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	5	8	theme	glycan	762:767	arg1	classes					769:775	glycan classes	762:775	glycan classes	762:775	The levels of neutral and acidic N-glycan compositions and glycan classes were analyzed in a total of ten different groups.					
32598367	2	9	theme	relevant	268:275	arg1	functions					258:266	many important functions	243:266	many important functions relevant to cancer	243:285	Glycans, the sugar moieties of glycoconjugates, are involved in many important functions relevant to cancer and can be of value as biomarkers.					
32598367	5	10	theme	neutral	717:723	arg1	compositions					745:756	N-glycan compositions	736:756	N-glycan compositions	736:756	The levels of neutral and acidic N-glycan compositions and glycan classes were analyzed in a total of ten different groups.					
32598367	8	11	theme	CRC	1557:1559	arg1	samples					1561:1567	CRC samples	1557:1567	CRC samples from the right and left colon	1557:1597	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	5	12	theme	acidic	729:734	arg1	compositions					745:756	N-glycan compositions	736:756	N-glycan compositions	736:756	The levels of neutral and acidic N-glycan compositions and glycan classes were analyzed in a total of ten different groups.					
32598367	7	13	theme	stage	1201:1205	arg1	samples					1218:1224	stage II and III samples	1201:1224	stage II and III samples	1201:1224	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	9	14	theme	glycans	1738:1744	arg1	levels					1717:1722	the levels	1713:1722	the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1	1713:1777	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	8	15	theme	right	1578:1582	arg1	colon					1593:1597	the right and left colon	1574:1597	the right and left colon	1574:1597	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	7	16	theme	classes	1188:1194	arg1	levels					1159:1164	the levels	1155:1164	the levels of glycans and glycan classes	1155:1194	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	2	17	theme	glycoconjugates	210:224	arg1	moieties					198:205	the sugar moieties	188:205	the sugar moieties of glycoconjugates	188:224	Glycans, the sugar moieties of glycoconjugates, are involved in many important functions relevant to cancer and can be of value as biomarkers.					
32598367	2	17	theme	glycoconjugates	210:224	arg1	Glycans					179:185	Glycans	179:185	Glycans	179:185	Glycans, the sugar moieties of glycoconjugates, are involved in many important functions relevant to cancer and can be of value as biomarkers.					
32598367	8	18	theme	left	1588:1591	arg1	colon					1593:1597	the right and left colon	1574:1597	the right and left colon	1574:1597	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	1	19	theme	cancer	136:141	arg1	types					127:131	many types	122:131	many types	122:131	Alterations in glycosylation are seen in many types of cancer, including colorectal cancer (CRC).					
32598367	1	19	theme	cancer	136:141	arg1	cancer					165:170	colorectal cancer	154:170	colorectal cancer (CRC)	154:176	Alterations in glycosylation are seen in many types of cancer, including colorectal cancer (CRC).					
32598367	3	20	from	patients	469:476	arg1	samples					448:454	10 healthy tissue samples	430:454	10 healthy tissue samples	430:454	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	3	20	from	patients	469:476	arg1	samples					418:424	35 CRC tissue samples	404:424	35 CRC tissue samples	404:424	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	3	20	from	patients	469:476	arg1	profiles					392:399	the N-glycan profiles	379:399	the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons	379:519	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	6	21	theme	significant	855:865	arg1	differences					867:877	no significant differences	852:877	no significant differences in glycan levels	852:894	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	6	22	from	differences	867:877	arg1	levels					889:894	glycan levels	882:894	glycan levels	882:894	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	8	23	theme	normal	1614:1619	arg1	variations					1621:1630	normal variations	1614:1630	normal variations in the levels of glycans between the healthy right and left colon	1614:1696	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	7	24	theme	glycan	1181:1186	arg1	classes					1188:1194	glycan classes	1181:1194	glycan classes	1181:1194	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	0	25	theme	N-glycomic	0:9	arg1	profiling					11:19	N-glycomic profiling	0:19	N-glycomic profiling of colorectal cancer	0:40	N-glycomic profiling of colorectal cancer according to tumor stage and location.					
32598367	5	26	theme	groups	819:824	arg1	total					796:800	a total	794:800	a total of ten different groups	794:824	The levels of neutral and acidic N-glycan compositions and glycan classes were analyzed in a total of ten different groups.					
32598367	7	27	theme	glycans	1169:1175	arg1	levels					1159:1164	the levels	1155:1164	the levels of glycans and glycan classes	1155:1194	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	3	28	theme	non-CRC	461:467	arg1	patients					469:476	non-CRC patients	461:476	non-CRC patients who underwent operations for other reasons	461:519	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	6	29	theme	CRC	927:929	arg1	samples					931:937	all right- and left-sided CRC samples	901:937	all right- and left-sided CRC samples	901:937	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	3	30	theme	mass	350:353	arg1	spectrometry					355:366	mass spectrometry	350:366	mass spectrometry	350:366	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	6	31	dep	differences	962:972	arg1	such					975:978	such	975:978	such	975:978	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	5	32	theme	N-glycan	736:743	arg1	compositions					745:756	N-glycan compositions	736:756	N-glycan compositions	736:756	The levels of neutral and acidic N-glycan compositions and glycan classes were analyzed in a total of ten different groups.					
32598367	0	33	theme	cancer	35:40	arg1	profiling					11:19	N-glycomic profiling	0:19	N-glycomic profiling of colorectal cancer	0:40	N-glycomic profiling of colorectal cancer according to tumor stage and location.					
32598367	9	34	from	levels	1895:1900	arg1	ways					1913:1916	similar ways	1905:1916	similar ways	1905:1916	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	8	35	theme	tissue	1385:1390	arg1	samples					1392:1398	healthy tissue samples	1377:1398	healthy tissue samples from the right and left colon	1377:1428	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	9	36	theme	location-nonspecific	1831:1850	arg1	manner					1852:1857	a cancer-specific but colon location-nonspecific manner	1803:1857	a cancer-specific but colon location-nonspecific manner	1803:1857	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	4	37	theme	right	682:686	arg1	colon					696:700	right or left colon	682:700	right or left colon	682:700	The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon.					
32598367	3	38	theme	tissue	411:416	arg1	samples					418:424	35 CRC tissue samples	404:424	35 CRC tissue samples	404:424	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	7	39	theme	progression	1317:1327	arg1	markers					1299:1305	new markers	1295:1305	new markers of cancer progression	1295:1327	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	7	40	theme	significant	1117:1127	arg1	differences					1129:1139	Multiple significant differences	1108:1139	Multiple significant differences	1108:1139	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	8	41	theme	right	1409:1413	arg1	colon					1424:1428	the right and left colon	1405:1428	the right and left colon	1405:1428	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	4	42	theme	left	691:694	arg1	colon					696:700	right or left colon	682:700	right or left colon	682:700	The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon.					
32598367	3	43	theme	samples	448:454	arg1	profiles					392:399	the N-glycan profiles	379:399	the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons	379:519	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	8	44	theme	left	1419:1422	arg1	colon					1424:1428	the right and left colon	1405:1428	the right and left colon	1405:1428	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	3	45	theme	healthy	433:439	arg1	samples					448:454	10 healthy tissue samples	430:454	10 healthy tissue samples	430:454	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	7	46	theme	new	1295:1297	arg1	markers					1299:1305	new markers	1295:1305	new markers of cancer progression	1295:1327	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	6	47	theme	few	958:960	arg1	differences					962:972	few differences	958:972	few differences (such as in the abundance of the neutral N-glycan H3N5)	958:1028	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	8	48	theme	glycans	1649:1655	arg1	levels					1639:1644	the levels	1635:1644	the levels of glycans between the healthy right and left colon	1635:1696	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	4	49	theme	tumor	526:530	arg1	samples					532:538	The tumor samples	522:538	The tumor samples	522:538	The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon.					
32598367	9	50	theme	similar	1905:1911	arg1	ways					1913:1916	similar ways	1905:1916	similar ways	1905:1916	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	2	51	theme	many	243:246	arg1	functions					258:266	many important functions	243:266	many important functions relevant to cancer	243:285	Glycans, the sugar moieties of glycoconjugates, are involved in many important functions relevant to cancer and can be of value as biomarkers.					
32598367	9	52	dep	glycans	1738:1744	arg1	glycans					1738:1744	the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1	1727:1777	the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1	1727:1777	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	9	52	dep	glycans	1738:1744	arg1	H5N4F1P1					1756:1763	H5N4F1P1	1756:1763	H5N4F1P1	1756:1763	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	9	52	dep	glycans	1738:1744	arg1	S1H5N4F1					1770:1777	S1H5N4F1	1770:1777	S1H5N4F1	1770:1777	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	9	52	dep	glycans	1738:1744	arg1	H4N3F1P1					1746:1753	H4N3F1P1	1746:1753	H4N3F1P1	1746:1753	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	1	53	theme	colorectal	154:163	arg1	CRC					173:175	CRC	173:175	CRC	173:175	Alterations in glycosylation are seen in many types of cancer, including colorectal cancer (CRC).					
32598367	1	53	theme	colorectal	154:163	arg1	cancer					165:170	colorectal cancer	154:170	colorectal cancer (CRC)	154:176	Alterations in glycosylation are seen in many types of cancer, including colorectal cancer (CRC).					
32598367	6	54	theme	H3N5	1024:1027	arg1	abundance					990:998	the abundance	986:998	the abundance of the neutral N-glycan H3N5	986:1027	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	8	55	theme	right	1677:1681	arg1	colon					1692:1696	the healthy right and left colon	1665:1696	the healthy right and left colon	1665:1696	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	3	56	theme	other	507:511	arg1	reasons					513:519	other reasons	507:519	other reasons	507:519	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	6	57	theme	neutral	1007:1013	arg1	H3N5					1024:1027	the neutral N-glycan H3N5	1003:1027	the neutral N-glycan H3N5	1003:1027	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	9	58	theme	tumor	1932:1936	arg1	location					1938:1945	tumor location	1932:1945	tumor location	1932:1945	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	8	59	from	colon	1424:1428	arg1	samples					1392:1398	healthy tissue samples	1377:1398	healthy tissue samples from the right and left colon	1377:1428	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	4	60	theme	tumor	578:582	arg1	location					584:591	tumor location	578:591	tumor location (right or left colon)	578:613	The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon.					
32598367	7	61	located	found	1146:1150	arg2	differences					1129:1139	Multiple significant differences	1108:1139	Multiple significant differences	1108:1139	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	7	61	located	found	1146:1150	arg1	levels					1159:1164	the levels	1155:1164	the levels of glycans and glycan classes	1155:1194	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	2	62	theme	sugar	192:196	arg1	moieties					198:205	the sugar moieties	188:205	the sugar moieties of glycoconjugates	188:224	Glycans, the sugar moieties of glycoconjugates, are involved in many important functions relevant to cancer and can be of value as biomarkers.					
32598367	2	62	theme	sugar	192:196	arg1	Glycans					179:185	Glycans	179:185	Glycans	179:185	Glycans, the sugar moieties of glycoconjugates, are involved in many important functions relevant to cancer and can be of value as biomarkers.					
32598367	8	63	theme	left	1687:1690	arg1	colon					1692:1696	the healthy right and left colon	1665:1696	the healthy right and left colon	1665:1696	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	7	64	theme	III	1214:1216	arg1	samples					1218:1224	stage II and III samples	1201:1224	stage II and III samples	1201:1224	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	9	65	theme	acidic	1731:1736	arg1	glycans					1738:1744	the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1	1727:1777	the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1	1727:1777	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	9	65	theme	acidic	1731:1736	arg1	H5N4F1P1					1756:1763	H5N4F1P1	1756:1763	H5N4F1P1	1756:1763	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	9	65	theme	acidic	1731:1736	arg1	S1H5N4F1					1770:1777	S1H5N4F1	1770:1777	S1H5N4F1	1770:1777	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	9	65	theme	acidic	1731:1736	arg1	H4N3F1P1					1746:1753	H4N3F1P1	1746:1753	H4N3F1P1	1746:1753	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	1	66	theme	many	122:125	arg1	types					127:131	many types	122:131	many types	122:131	Alterations in glycosylation are seen in many types of cancer, including colorectal cancer (CRC).					
32598367	1	66	theme	many	122:125	arg1	cancer					165:170	colorectal cancer	154:170	colorectal cancer (CRC)	154:176	Alterations in glycosylation are seen in many types of cancer, including colorectal cancer (CRC).					
32598367	6	67	theme	right-	905:910	arg1	samples					931:937	all right- and left-sided CRC samples	901:937	all right- and left-sided CRC samples	901:937	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	8	68	from	colon	1593:1597	arg1	samples					1561:1567	CRC samples	1557:1567	CRC samples from the right and left colon	1557:1597	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	5	69	theme	classes	769:775	arg1	levels					707:712	The levels	703:712	The levels of neutral and acidic N-glycan compositions and glycan classes	703:775	The levels of neutral and acidic N-glycan compositions and glycan classes were analyzed in a total of ten different groups.					
32598367	8	70	from	variations	1621:1630	arg1	levels					1639:1644	the levels	1635:1644	the levels of glycans between the healthy right and left colon	1635:1696	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	6	71	theme	glycan	882:887	arg1	levels					889:894	glycan levels	882:894	glycan levels	882:894	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	4	72	dep	stage	619:623	arg1	II					626:627	II	626:627	II	626:627	The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon.					
32598367	4	72	dep	stage	619:623	arg1	III					632:634	III	632:634	III	632:634	The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon.					
32598367	4	73	theme	healthy	648:654	arg1	samples					656:662	the healthy samples	644:662	the healthy samples	644:662	The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon.					
32598367	8	74	from	differences	1455:1465	arg1	levels					1474:1479	the levels	1470:1479	the levels of any of the glycans analyzed	1470:1510	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	3	75	theme	N-glycan	383:390	arg1	profiles					392:399	the N-glycan profiles	379:399	the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons	379:519	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	5	76	theme	different	809:817	arg1	groups					819:824	ten different groups	805:824	ten different groups	805:824	The levels of neutral and acidic N-glycan compositions and glycan classes were analyzed in a total of ten different groups.					
32598367	0	77	theme	colorectal	24:33	arg1	cancer					35:40	colorectal cancer	24:40	colorectal cancer	24:40	N-glycomic profiling of colorectal cancer according to tumor stage and location.					
32598367	8	78	theme	healthy	1377:1383	arg1	samples					1392:1398	healthy tissue samples	1377:1398	healthy tissue samples from the right and left colon	1377:1428	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
32598367	9	79	theme	cancer-specific	1805:1819	arg1	manner					1852:1857	a cancer-specific but colon location-nonspecific manner	1803:1857	a cancer-specific but colon location-nonspecific manner	1803:1857	Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.					
32598367	3	80	theme	CRC	407:409	arg1	samples					418:424	35 CRC tissue samples	404:424	35 CRC tissue samples	404:424	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	4	81	dep	location	584:591	arg1	right					594:598	right	594:598	right	594:598	The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon.					
32598367	4	81	dep	location	584:591	arg1	left					603:606	left	603:606	left	603:606	The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon.					
32598367	6	82	theme	left-sided	916:925	arg1	samples					931:937	all right- and left-sided CRC samples	901:937	all right- and left-sided CRC samples	901:937	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	3	83	from	profiles	392:399	arg1	patients					469:476	non-CRC patients	461:476	non-CRC patients who underwent operations for other reasons	461:519	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	0	84	theme	tumor	55:59	arg1	stage					61:65	tumor stage	55:65	tumor stage	55:65	N-glycomic profiling of colorectal cancer according to tumor stage and location.					
32598367	3	85	used	used	345:348	arg2	we					337:338	we	337:338	we	337:338	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	6	86	from	abundance	990:998	arg1	such					975:978	such	975:978	such	975:978	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	7	87	theme	Multiple	1108:1115	arg1	differences					1129:1139	Multiple significant differences	1108:1139	Multiple significant differences	1108:1139	Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression.					
32598367	3	88	theme	samples	418:424	arg1	profiles					392:399	the N-glycan profiles	379:399	the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons	379:519	In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons.					
32598367	1	89	from	Alterations	81:91	arg1	glycosylation					96:108	glycosylation	96:108	glycosylation	96:108	Alterations in glycosylation are seen in many types of cancer, including colorectal cancer (CRC).					
32598367	4	90	dep	right	594:598	arg1	colon					608:612	colon	608:612	colon	608:612	The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon.					
32598367	6	91	from	such	975:978	arg1	abundance					990:998	the abundance	986:998	the abundance of the neutral N-glycan H3N5	986:1027	Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage.					
32598367	8	92	theme	any	1484:1486	arg1	levels					1474:1479	the levels	1470:1479	the levels of any of the glycans analyzed	1470:1510	In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon.					
33858135	0	0	theme	Molecular	80:88	arg1	Simulation					99:108	Molecular Dynamics Simulation	80:108	Molecular Dynamics Simulation	80:108	Mechanism of Glycans Modulating Cholesteryl Ester Transfer Protein: Unveiled by Molecular Dynamics Simulation.					
33858135	2	1	from	doubts	403:408	arg1	drugability					424:434	the target drugability	413:434	the target drugability of CETP	413:442	However, clinical trials of several CETP inhibitors with various potencies have been marginally successful at best, raising doubts on the target drugability of CETP.					
33858135	7	2	theme	similar	1345:1351	arg1	regions					1353:1359	similar regions	1345:1359	similar regions	1345:1359	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	8	3	theme	CVD	1525:1527	arg1	treatment					1529:1537	CVD treatment	1525:1537	CVD treatment targeting CETP and functional studies about glycosylation for other systems	1525:1613	This study would provide valuable insights into devising novel methods for CVD treatment targeting CETP and functional studies about glycosylation for other systems.					
33858135	1	4	theme	cardiovascular	231:244	arg1	disease					246:252	cardiovascular disease	231:252	cardiovascular disease (CVD)	231:258	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	1	4	theme	cardiovascular	231:244	arg1	CVD					255:257	CVD	255:257	CVD	255:257	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	5	5	theme	extensive	756:764	arg1	results					777:783	the extensive simulation results	752:783	the extensive simulation results	752:783	Here, the extensive simulation results intensely suggest that glycan88 tends to assist CETP in forming a continuous tunnel throughout interacting with the upper-right region of the N-barrel, while it also could prevent the formation of a continuous tunnel by swinging toward the right-rear of the N-barrel.					
33858135	2	6	theme	inhibitors	320:329	arg1	trials					297:302	clinical trials	288:302	clinical trials of several CETP inhibitors with various potencies	288:352	However, clinical trials of several CETP inhibitors with various potencies have been marginally successful at best, raising doubts on the target drugability of CETP.					
33858135	8	7	theme	novel	1507:1511	arg1	methods					1513:1519	novel methods	1507:1519	novel methods	1507:1519	This study would provide valuable insights into devising novel methods for CVD treatment targeting CETP and functional studies about glycosylation for other systems.					
33858135	1	8	theme	disease	246:252	arg1	treatment					218:226	the treatment	214:226	the treatment of cardiovascular disease (CVD) for three decades	214:276	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	7	9	theme	nonspecific	1184:1194	arg1	involvement					1196:1206	the nonspecific involvement	1180:1206	the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions	1180:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	10	theme	glycans	1326:1332	arg1	influence					1303:1311	the similar influence	1291:1311	the similar influence of different glycans trapped at similar regions on the protein structure	1291:1384	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	10	theme	glycans	1326:1332	arg1	involvement					1196:1206	the nonspecific involvement	1180:1206	the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions	1180:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	11	theme	physiological	1399:1411	arg1	glycan					1413:1418	physiological glycan	1399:1418	physiological glycan	1399:1418	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	0	12	theme	Dynamics	90:97	arg1	Simulation					99:108	Molecular Dynamics Simulation	80:108	Molecular Dynamics Simulation	80:108	Mechanism of Glycans Modulating Cholesteryl Ester Transfer Protein: Unveiled by Molecular Dynamics Simulation.					
33858135	6	13	theme	central	1143:1149	arg1	cavity					1151:1156	the central cavity	1139:1156	the central cavity of CETP	1139:1164	Furthermore, glycan240 formed stable H-bonds with Helix-B and might further stabilize the central cavity of CETP.					
33858135	7	14	with	glycans	1248:1254	arg1	interactions					1274:1285	protein core interactions	1261:1285	protein core interactions	1261:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	15	theme	similar	1434:1440	arg1	effect					1442:1447	a similar effect	1432:1447	a similar effect	1432:1447	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	3	16	theme	in-depth	449:456	arg1	understanding					458:470	The in-depth understanding	445:470	The in-depth understanding of the glycosylated CETP structure	445:505	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	3	16	theme	in-depth	449:456	arg1	beneficial					516:525	beneficial	516:525	beneficial	516:525	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	7	17	theme	core	1269:1272	arg1	interactions					1274:1285	protein core interactions	1261:1285	protein core interactions	1261:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	18	from	glycans	1248:1254	arg1	influence					1303:1311	the similar influence	1291:1311	the similar influence of different glycans trapped at similar regions on the protein structure	1291:1384	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	18	from	glycans	1248:1254	arg1	involvement					1196:1206	the nonspecific involvement	1180:1206	the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions	1180:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	18	from	glycans	1248:1254	arg1	groups					1224:1229	the hydroxyl groups	1211:1229	the hydroxyl groups from the various glycans with protein core interactions	1211:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	3	19	gly	glycosylated	479:490	arg1	structure					497:505	the glycosylated CETP structure	475:505	the glycosylated CETP structure	475:505	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	4	20	theme	molecular	636:644	arg1	simulations					655:665	large-scale molecular dynamics simulations	624:665	large-scale molecular dynamics simulations	624:665	In this work, large-scale molecular dynamics simulations were performed to thoroughly explore the mechanism of glycans modulating CETP.					
33858135	5	21	theme	upper-right	901:911	arg1	region					913:918	the upper-right region	897:918	the upper-right region of the N-barrel	897:934	Here, the extensive simulation results intensely suggest that glycan88 tends to assist CETP in forming a continuous tunnel throughout interacting with the upper-right region of the N-barrel, while it also could prevent the formation of a continuous tunnel by swinging toward the right-rear of the N-barrel.					
33858135	5	22	theme	simulation	766:775	arg1	results					777:783	the extensive simulation results	752:783	the extensive simulation results	752:783	Here, the extensive simulation results intensely suggest that glycan88 tends to assist CETP in forming a continuous tunnel throughout interacting with the upper-right region of the N-barrel, while it also could prevent the formation of a continuous tunnel by swinging toward the right-rear of the N-barrel.					
33858135	4	23	theme	large-scale	624:634	arg1	simulations					655:665	large-scale molecular dynamics simulations	624:665	large-scale molecular dynamics simulations	624:665	In this work, large-scale molecular dynamics simulations were performed to thoroughly explore the mechanism of glycans modulating CETP.					
33858135	2	24	theme	target	417:422	arg1	drugability					424:434	the target drugability	413:434	the target drugability of CETP	413:442	However, clinical trials of several CETP inhibitors with various potencies have been marginally successful at best, raising doubts on the target drugability of CETP.					
33858135	3	25	theme	structure	497:505	arg1	understanding					458:470	The in-depth understanding	445:470	The in-depth understanding of the glycosylated CETP structure	445:505	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	3	25	theme	structure	497:505	arg1	beneficial					516:525	beneficial	516:525	beneficial	516:525	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	7	26	theme	various	1240:1246	arg1	glycans					1248:1254	the various glycans	1236:1254	the various glycans with protein core interactions	1236:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	2	27	theme	clinical	288:295	arg1	trials					297:302	clinical trials	288:302	clinical trials of several CETP inhibitors with various potencies	288:352	However, clinical trials of several CETP inhibitors with various potencies have been marginally successful at best, raising doubts on the target drugability of CETP.					
33858135	7	28	theme	protein	1261:1267	arg1	interactions					1274:1285	protein core interactions	1261:1285	protein core interactions	1261:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	8	29	theme	valuable	1475:1482	arg1	insights					1484:1491	valuable insights	1475:1491	valuable insights into devising novel methods for CVD treatment targeting CETP and functional studies about glycosylation for other systems	1475:1613	This study would provide valuable insights into devising novel methods for CVD treatment targeting CETP and functional studies about glycosylation for other systems.					
33858135	4	30	theme	glycans	721:727	arg1	mechanism					708:716	the mechanism	704:716	the mechanism of glycans modulating CETP	704:743	In this work, large-scale molecular dynamics simulations were performed to thoroughly explore the mechanism of glycans modulating CETP.					
33858135	4	31	theme	dynamics	646:653	arg1	simulations					655:665	large-scale molecular dynamics simulations	624:665	large-scale molecular dynamics simulations	624:665	In this work, large-scale molecular dynamics simulations were performed to thoroughly explore the mechanism of glycans modulating CETP.					
33858135	0	32	theme	Modulating	21:30	arg1	Protein					59:65	Modulating Cholesteryl Ester Transfer Protein	21:65	Glycans Modulating Cholesteryl Ester Transfer Protein	13:65	Mechanism of Glycans Modulating Cholesteryl Ester Transfer Protein: Unveiled by Molecular Dynamics Simulation.					
33858135	3	33	theme	CETP	566:569	arg1	function					571:578	the CETP function	562:578	the CETP function	562:578	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	7	34	theme	similar	1295:1301	arg1	influence					1303:1311	the similar influence	1291:1311	the similar influence of different glycans trapped at similar regions on the protein structure	1291:1384	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	0	35	theme	Glycans	13:19	arg1	Mechanism					0:8	Mechanism	0:8	Mechanism of Glycans Modulating Cholesteryl Ester Transfer Protein:	0:66	Mechanism of Glycans Modulating Cholesteryl Ester Transfer Protein: Unveiled by Molecular Dynamics Simulation.					
33858135	6	36	theme	CETP	1161:1164	arg1	cavity					1151:1156	the central cavity	1139:1156	the central cavity of CETP	1139:1164	Furthermore, glycan240 formed stable H-bonds with Helix-B and might further stabilize the central cavity of CETP.					
33858135	3	37	theme	definitive	535:544	arg1	descriptions					546:557	more definitive descriptions	530:557	more definitive descriptions of the CETP function and the underlying mechanism	530:607	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	3	38	theme	CETP	492:495	arg1	structure					497:505	the glycosylated CETP structure	475:505	the glycosylated CETP structure	475:505	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	0	39	theme	Ester	44:48	arg1	Protein					59:65	Modulating Cholesteryl Ester Transfer Protein	21:65	Glycans Modulating Cholesteryl Ester Transfer Protein	13:65	Mechanism of Glycans Modulating Cholesteryl Ester Transfer Protein: Unveiled by Molecular Dynamics Simulation.					
33858135	8	40	theme	functional	1558:1567	arg1	studies					1569:1575	functional studies	1558:1575	functional studies	1558:1575	This study would provide valuable insights into devising novel methods for CVD treatment targeting CETP and functional studies about glycosylation for other systems.					
33858135	2	41	with	trials	297:302	arg1	potencies					344:352	various potencies	336:352	various potencies	336:352	However, clinical trials of several CETP inhibitors with various potencies have been marginally successful at best, raising doubts on the target drugability of CETP.					
33858135	3	42	theme	function	571:578	arg1	descriptions					546:557	more definitive descriptions	530:557	more definitive descriptions of the CETP function and the underlying mechanism	530:607	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	7	43	theme	different	1316:1324	arg1	glycans					1326:1332	different glycans	1316:1332	different glycans trapped at similar regions	1316:1359	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	1	44	theme	promising	196:204	arg1	Inhibition					111:120	Inhibition	111:120	Inhibition of the cholesteryl ester transfer protein (CETP)	111:169	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	1	44	theme	promising	196:204	arg1	way					206:208	a promising way	194:208	a promising way for the treatment of cardiovascular disease (CVD) for three decades	194:276	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	2	45	theme	CETP	439:442	arg1	drugability					424:434	the target drugability	413:434	the target drugability of CETP	413:442	However, clinical trials of several CETP inhibitors with various potencies have been marginally successful at best, raising doubts on the target drugability of CETP.					
33858135	0	46	theme	Cholesteryl	32:42	arg1	Protein					59:65	Modulating Cholesteryl Ester Transfer Protein	21:65	Glycans Modulating Cholesteryl Ester Transfer Protein	13:65	Mechanism of Glycans Modulating Cholesteryl Ester Transfer Protein: Unveiled by Molecular Dynamics Simulation.					
33858135	7	47	theme	protein	1368:1374	arg1	structure					1376:1384	the protein structure	1364:1384	the protein structure	1364:1384	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	48	from	involvement	1196:1206	arg1	glycans					1248:1254	the various glycans	1236:1254	the various glycans with protein core interactions	1236:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	48	from	involvement	1196:1206	arg1	structure					1376:1384	the protein structure	1364:1384	the protein structure	1364:1384	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	2	49	theme	CETP	315:318	arg1	inhibitors					320:329	several CETP inhibitors	307:329	several CETP inhibitors	307:329	However, clinical trials of several CETP inhibitors with various potencies have been marginally successful at best, raising doubts on the target drugability of CETP.					
33858135	0	50	theme	Transfer	50:57	arg1	Protein					59:65	Modulating Cholesteryl Ester Transfer Protein	21:65	Glycans Modulating Cholesteryl Ester Transfer Protein	13:65	Mechanism of Glycans Modulating Cholesteryl Ester Transfer Protein: Unveiled by Molecular Dynamics Simulation.					
33858135	8	51	theme	other	1601:1605	arg1	systems					1607:1613	other systems	1601:1613	other systems	1601:1613	This study would provide valuable insights into devising novel methods for CVD treatment targeting CETP and functional studies about glycosylation for other systems.					
33858135	1	52	theme	protein	156:162	arg1	way					206:208	a promising way	194:208	a promising way for the treatment of cardiovascular disease (CVD) for three decades	194:276	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	1	52	theme	protein	156:162	arg1	Inhibition					111:120	Inhibition	111:120	Inhibition of the cholesteryl ester transfer protein (CETP)	111:169	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	3	53	theme	glycosylated	479:490	arg1	structure					497:505	the glycosylated CETP structure	475:505	the glycosylated CETP structure	475:505	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	2	54	theme	several	307:313	arg1	inhibitors					320:329	several CETP inhibitors	307:329	several CETP inhibitors	307:329	However, clinical trials of several CETP inhibitors with various potencies have been marginally successful at best, raising doubts on the target drugability of CETP.					
33858135	5	55	theme	tunnel	995:1000	arg1	formation					969:977	the formation	965:977	the formation of a continuous tunnel	965:1000	Here, the extensive simulation results intensely suggest that glycan88 tends to assist CETP in forming a continuous tunnel throughout interacting with the upper-right region of the N-barrel, while it also could prevent the formation of a continuous tunnel by swinging toward the right-rear of the N-barrel.					
33858135	3	56	theme	mechanism	599:607	arg1	descriptions					546:557	more definitive descriptions	530:557	more definitive descriptions of the CETP function and the underlying mechanism	530:607	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	7	57	from	influence	1303:1311	arg1	glycans					1248:1254	the various glycans	1236:1254	the various glycans with protein core interactions	1236:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	57	from	influence	1303:1311	arg1	structure					1376:1384	the protein structure	1364:1384	the protein structure	1364:1384	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	1	58	theme	cholesteryl	129:139	arg1	protein					156:162	cholesteryl ester transfer protein	129:162	the cholesteryl ester transfer protein (CETP)	125:169	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	1	58	theme	cholesteryl	129:139	arg1	CETP					165:168	CETP	165:168	CETP	165:168	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	0	59	dep	Glycans	13:19	arg1	Protein					59:65	Modulating Cholesteryl Ester Transfer Protein	21:65	Glycans Modulating Cholesteryl Ester Transfer Protein	13:65	Mechanism of Glycans Modulating Cholesteryl Ester Transfer Protein: Unveiled by Molecular Dynamics Simulation.					
33858135	2	60	from	best	389:392	arg1	successful					375:384	successful	375:384	successful	375:384	However, clinical trials of several CETP inhibitors with various potencies have been marginally successful at best, raising doubts on the target drugability of CETP.					
33858135	1	61	theme	ester	141:145	arg1	protein					156:162	cholesteryl ester transfer protein	129:162	the cholesteryl ester transfer protein (CETP)	125:169	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	1	61	theme	ester	141:145	arg1	CETP					165:168	CETP	165:168	CETP	165:168	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	5	62	theme	N-barrel	927:934	arg1	region					913:918	the upper-right region	897:918	the upper-right region of the N-barrel	897:934	Here, the extensive simulation results intensely suggest that glycan88 tends to assist CETP in forming a continuous tunnel throughout interacting with the upper-right region of the N-barrel, while it also could prevent the formation of a continuous tunnel by swinging toward the right-rear of the N-barrel.					
33858135	7	63	theme	hydroxyl	1215:1222	arg1	groups					1224:1229	the hydroxyl groups	1211:1229	the hydroxyl groups from the various glycans with protein core interactions	1211:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	5	64	theme	N-barrel	1043:1050	arg1	right-rear					1025:1034	the right-rear	1021:1034	the right-rear of the N-barrel	1021:1050	Here, the extensive simulation results intensely suggest that glycan88 tends to assist CETP in forming a continuous tunnel throughout interacting with the upper-right region of the N-barrel, while it also could prevent the formation of a continuous tunnel by swinging toward the right-rear of the N-barrel.					
33858135	5	65	theme	continuous	984:993	arg1	tunnel					995:1000	a continuous tunnel	982:1000	a continuous tunnel	982:1000	Here, the extensive simulation results intensely suggest that glycan88 tends to assist CETP in forming a continuous tunnel throughout interacting with the upper-right region of the N-barrel, while it also could prevent the formation of a continuous tunnel by swinging toward the right-rear of the N-barrel.					
33858135	1	66	theme	transfer	147:154	arg1	protein					156:162	cholesteryl ester transfer protein	129:162	the cholesteryl ester transfer protein (CETP)	125:169	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	1	66	theme	transfer	147:154	arg1	CETP					165:168	CETP	165:168	CETP	165:168	Inhibition of the cholesteryl ester transfer protein (CETP) has been considered as a promising way for the treatment of cardiovascular disease (CVD) for three decades.					
33858135	5	67	theme	continuous	851:860	arg1	tunnel					862:867	a continuous tunnel	849:867	a continuous tunnel	849:867	Here, the extensive simulation results intensely suggest that glycan88 tends to assist CETP in forming a continuous tunnel throughout interacting with the upper-right region of the N-barrel, while it also could prevent the formation of a continuous tunnel by swinging toward the right-rear of the N-barrel.					
33858135	7	68	theme	groups	1224:1229	arg1	influence					1303:1311	the similar influence	1291:1311	the similar influence of different glycans trapped at similar regions on the protein structure	1291:1384	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	7	68	theme	groups	1224:1229	arg1	involvement					1196:1206	the nonspecific involvement	1180:1206	the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions	1180:1285	Furthermore, the nonspecific involvement of the hydroxyl groups from the various glycans with protein core interactions and the similar influence of different glycans trapped at similar regions on the protein structure suggest that physiological glycan may lead to a similar effect.					
33858135	3	69	theme	underlying	588:597	arg1	mechanism					599:607	the underlying mechanism	584:607	the underlying mechanism	584:607	The in-depth understanding of the glycosylated CETP structure could be beneficial to more definitive descriptions of the CETP function and the underlying mechanism.					
33858135	2	70	theme	various	336:342	arg1	potencies					344:352	various potencies	336:352	various potencies	336:352	However, clinical trials of several CETP inhibitors with various potencies have been marginally successful at best, raising doubts on the target drugability of CETP.					
32343899	4	0	theme	N	824:824	arg1	conditions					869:878	N,N-dimethylformamide-directed glycosylation conditions	824:878	conditions	869:878	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	1	1	theme	difficult-to-separate	149:169	arg1	components					187:196	several difficult-to-separate major and minor components	141:196	several difficult-to-separate major and minor components	141:196	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	2	2	from	nephro-	326:332	arg1	activity					312:319	separate antibacterial activity	289:319	separate antibacterial activity from nephro- and/or ototoxicity	289:351	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	4	3	theme	acceptor	639:646	arg1	Glycosylation					617:629	Glycosylation	617:629	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor	617:711	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	1	4	theme	major	171:175	arg1	components					187:196	several difficult-to-separate major and minor components	141:196	several difficult-to-separate major and minor components	141:196	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	2	5	from	ototoxicity	341:351	arg1	activity					312:319	separate antibacterial activity	289:319	separate antibacterial activity from nephro- and/or ototoxicity	289:351	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	4	6	with	Glycosylation	617:629	arg1	donor					707:711	a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor	653:711	a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor	653:711	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	3	7	theme	available	596:604	arg1	sisomicin					606:614	readily available sisomicin	588:614	readily available sisomicin	588:614	With a view to facilitating such studies, the synthesis of a fully and selectively protected garamine-based acceptor has been developed from readily available sisomicin.					
32343899	2	8	theme	antibacterial	298:310	arg1	activity					312:319	separate antibacterial activity	289:319	separate antibacterial activity from nephro- and/or ototoxicity	289:351	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	1	9	theme	components	187:196	arg1	gentamicin					114:123	The clinical aminoglycoside antibiotic gentamicin	75:123	The clinical aminoglycoside antibiotic gentamicin	75:123	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	1	9	theme	components	187:196	arg1	mixture					130:136	a mixture	128:136	a mixture of several difficult-to-separate major and minor components	128:196	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	2	10	from	inaccessibility	212:226	arg1	particular					255:264	particular	255:264	particular	255:264	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	2	11	theme	separate	289:296	arg1	activity					312:319	separate antibacterial activity	289:319	separate antibacterial activity from nephro- and/or ototoxicity	289:351	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	1	12	theme	minor	181:185	arg1	components					187:196	several difficult-to-separate major and minor components	141:196	several difficult-to-separate major and minor components	141:196	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	4	13	theme	gentamicin	888:897	arg1	X2					899:900	gentamicin X2	888:900	gentamicin X2 after deprotection	888:919	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	2	14	from	components	241:250	arg1	particular					255:264	particular	255:264	particular	255:264	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	2	15	theme	read-through	424:435	arg1	activity					437:444	the potentially therapeutically important read-through activity	382:444	the potentially therapeutically important read-through activity	382:444	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	1	16	theme	clinical	79:86	arg1	gentamicin					114:123	The clinical aminoglycoside antibiotic gentamicin	75:123	The clinical aminoglycoside antibiotic gentamicin	75:123	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	1	16	theme	clinical	79:86	arg1	mixture					130:136	a mixture	128:136	a mixture of several difficult-to-separate major and minor components	128:196	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	2	17	theme	important	414:422	arg1	activity					437:444	the potentially therapeutically important read-through activity	382:444	the potentially therapeutically important read-through activity	382:444	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	0	18	theme	Minor	24:28	arg1	Components					30:39	Gentamicin Minor Components	13:39	Gentamicin Minor Components	13:39	Synthesis of Gentamicin Minor Components: Gentamicin B1 and Gentamicin X2.					
32343899	1	19	theme	aminoglycoside	88:101	arg1	gentamicin					114:123	The clinical aminoglycoside antibiotic gentamicin	75:123	The clinical aminoglycoside antibiotic gentamicin	75:123	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	1	19	theme	aminoglycoside	88:101	arg1	mixture					130:136	a mixture	128:136	a mixture of several difficult-to-separate major and minor components	128:196	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	2	20	from	particular	255:264	arg1	inaccessibility					212:226	The relative inaccessibility	199:226	The relative inaccessibility of the minor components in particular	199:264	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	0	21	theme	Gentamicin	13:22	arg1	Components					30:39	Gentamicin Minor Components	13:39	Gentamicin Minor Components	13:39	Synthesis of Gentamicin Minor Components: Gentamicin B1 and Gentamicin X2.					
32343899	4	22	gly	Glycosylation	617:629	arg1	acceptor					639:646	this acceptor	634:646	this acceptor	634:646	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	1	23	theme	antibiotic	103:112	arg1	gentamicin					114:123	The clinical aminoglycoside antibiotic gentamicin	75:123	The clinical aminoglycoside antibiotic gentamicin	75:123	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	1	23	theme	antibiotic	103:112	arg1	mixture					130:136	a mixture	128:136	a mixture of several difficult-to-separate major and minor components	128:196	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
32343899	3	24	theme	garamine-based	540:553	arg1	acceptor					555:562	a fully and selectively protected garamine-based acceptor	506:562	a fully and selectively protected garamine-based acceptor	506:562	With a view to facilitating such studies, the synthesis of a fully and selectively protected garamine-based acceptor has been developed from readily available sisomicin.					
32343899	3	25	theme	such	475:478	arg1	studies					480:486	such studies	475:486	such studies	475:486	With a view to facilitating such studies, the synthesis of a fully and selectively protected garamine-based acceptor has been developed from readily available sisomicin.					
32343899	0	26	theme	Components	30:39	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Gentamicin Minor Components: Gentamicin B1 and Gentamicin X2.	0:73	Synthesis of Gentamicin Minor Components: Gentamicin B1 and Gentamicin X2.					
32343899	2	27	theme	components	241:250	arg1	inaccessibility					212:226	The relative inaccessibility	199:226	The relative inaccessibility of the minor components in particular	199:264	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	2	28	theme	minor	235:239	arg1	components					241:250	the minor components	231:250	the minor components in particular	231:264	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	4	29	theme	N-dimethylformamide-directed	826:853	arg1	conditions					869:878	N,N-dimethylformamide-directed glycosylation conditions	824:878	conditions	869:878	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	0	30	theme	Gentamicin	42:51	arg1	B1					53:54	Gentamicin B1	42:54	Gentamicin B1	42:54	Synthesis of Gentamicin Minor Components: Gentamicin B1 and Gentamicin X2.					
32343899	3	31	theme	acceptor	555:562	arg1	synthesis					493:501	the synthesis	489:501	the synthesis of a fully and selectively protected garamine-based acceptor	489:562	With a view to facilitating such studies, the synthesis of a fully and selectively protected garamine-based acceptor has been developed from readily available sisomicin.					
32343899	3	32	theme	protected	530:538	arg1	acceptor					555:562	a fully and selectively protected garamine-based acceptor	506:562	a fully and selectively protected garamine-based acceptor	506:562	With a view to facilitating such studies, the synthesis of a fully and selectively protected garamine-based acceptor has been developed from readily available sisomicin.					
32343899	4	33	dep	affords	713:719	arg1	whereas					755:761	whereas	755:761	whereas	755:761	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	4	34	theme	2-azido-2-deoxy-d-glucopyranosyl	779:810	arg1	donor					812:816	a 2-azido-2-deoxy-d-glucopyranosyl donor	777:816	a 2-azido-2-deoxy-d-glucopyranosyl donor	777:816	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	2	35	theme	relative	203:210	arg1	inaccessibility					212:226	The relative inaccessibility	199:226	The relative inaccessibility of the minor components in particular	199:264	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	4	36	theme	glycosylation	855:867	arg1	conditions					869:878	N,N-dimethylformamide-directed glycosylation conditions	824:878	conditions	869:878	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	4	37	theme	6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl	655:705	arg1	donor					707:711	a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor	653:711	a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor	653:711	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	0	38	theme	Gentamicin	60:69	arg1	X2					71:72	Gentamicin X2	60:72	Gentamicin X2	60:72	Synthesis of Gentamicin Minor Components: Gentamicin B1 and Gentamicin X2.					
32343899	4	39	theme	donor	812:816	arg1	employment					763:772	employment	763:772	employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions	763:878	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	2	40	theme	activity	437:444	arg1	origin					372:377	the origin	368:377	the origin of the potentially therapeutically important read-through activity	368:444	The relative inaccessibility of the minor components in particular complicates efforts to separate antibacterial activity from nephro- and/or ototoxicity and to clarify the origin of the potentially therapeutically important read-through activity.					
32343899	4	41	theme	gentamicin	721:730	arg1	B1					732:733	gentamicin B1	721:733	gentamicin B1 after deprotection	721:752	Glycosylation of this acceptor with a 6-azido-6,7-dideoxy-d-glycero-d-glucoheptopyranosyl donor affords gentamicin B1 after deprotection, whereas employment of a 2-azido-2-deoxy-d-glucopyranosyl donor under N,N-dimethylformamide-directed glycosylation conditions affords gentamicin X2 after deprotection.					
32343899	0	42	dep	Synthesis	0:8	arg1	X2					71:72	Gentamicin X2	60:72	Gentamicin X2	60:72	Synthesis of Gentamicin Minor Components: Gentamicin B1 and Gentamicin X2.					
32343899	0	42	dep	Synthesis	0:8	arg1	B1					53:54	Gentamicin B1	42:54	Gentamicin B1	42:54	Synthesis of Gentamicin Minor Components: Gentamicin B1 and Gentamicin X2.					
32343899	1	43	theme	several	141:147	arg1	components					187:196	several difficult-to-separate major and minor components	141:196	several difficult-to-separate major and minor components	141:196	The clinical aminoglycoside antibiotic gentamicin is a mixture of several difficult-to-separate major and minor components.					
34687008	3	0	theme	complexity	756:765	arg1	levels					767:772	several complexity levels	748:772	several complexity levels	748:772	This chapter summarizes mass spectrometry basics, focusing on IgG and monoclonal antibody N-glycosylation analysis on several complexity levels.					
34687008	4	1	theme	recent	934:939	arg1	breakthroughs					941:953	recent breakthroughs	934:953	recent breakthroughs	934:953	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	5	2	theme	IgG	1081:1083	arg1	ionization					1185:1194	matrix-assisted laser desorption ionization	1152:1194	matrix-assisted laser desorption ionization	1152:1194	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	5	2	theme	IgG	1081:1083	arg1	spectrometry					1135:1146	liquid chromatography-mass spectrometry	1108:1146	liquid chromatography-mass spectrometry	1108:1146	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	5	2	theme	IgG	1081:1083	arg1	glycoanalytics					1085:1098	IgG glycoanalytics	1081:1098	IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization	1081:1194	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	3	theme	glycopeptide	372:383	arg1	demands					395:401	immunoglobulin G (IgG) glycan and glycopeptide profiling demands	338:401	demands	395:401	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	2	4	from	advances	478:485	arg1	field					494:498	the field	490:498	the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization	490:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	5	5	dep	terms	1240:1244	arg1	either					1290:1295	either	1290:1295	either	1290:1295	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	1	6	theme	capillary	181:189	arg1	electrophoresis					191:205	capillary electrophoresis	181:205	capillary electrophoresis	181:205	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	7	theme	robust	277:282	arg1	analysis					319:326	robust and reliable protein glycosylation analysis	277:326	robust and reliable protein glycosylation analysis	277:326	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	4	8	theme	glycan	828:833	arg1	release					835:841	glycan release	828:841	glycan release	828:841	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	5	9	theme	selected	1040:1047	arg1	methodologies					1064:1076	selected highly popular methodologies	1040:1076	selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization	1040:1194	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	10	theme	preparation	542:552	arg1	terms					503:507	terms	503:507	terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization	503:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	5	11	theme	popular	1056:1062	arg1	methodologies					1064:1076	selected highly popular methodologies	1040:1076	selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization	1040:1194	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	1	12	theme	Mass	79:82	arg1	spectrometry					84:95	Mass spectrometry	79:95	Mass spectrometry	79:95	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	12	theme	Mass	79:82	arg1	cornerstone					262:272	a cornerstone	260:272	a cornerstone of robust and reliable protein glycosylation analysis	260:326	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	12	theme	Mass	79:82	arg1	techniques					116:125	its hyphenated techniques	101:125	its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes	101:248	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	13	theme	reliable	288:295	arg1	analysis					319:326	robust and reliable protein glycosylation analysis	277:326	robust and reliable protein glycosylation analysis	277:326	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	2	14	theme	sample	535:540	arg1	preparation					542:552	sample preparation	535:552	sample preparation	535:552	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	5	15	from	beginner	1305:1312	arg1	field					1321:1325	the field	1317:1325	the field	1317:1325	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	16	theme	biomedical	420:429	arg1	applications					444:455	both applied biomedical and research applications	407:455	both applied biomedical and research applications	407:455	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	4	17	gly	glycopeptide	858:869	arg2	glycopeptide					858:869	glycopeptide preparation	858:881	glycopeptide preparation	858:881	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	5	18	theme	simple	1233:1238	arg1	terms					1240:1244	simple terms	1233:1244	simple terms making this text a practical starting point	1233:1288	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	1	19	from	improvements	142:153	arg1	chromatography					165:178	liquid chromatography	158:178	liquid chromatography	158:178	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	19	from	improvements	142:153	arg1	electrophoresis					191:205	capillary electrophoresis	181:205	capillary electrophoresis	181:205	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	19	from	improvements	142:153	arg1	ionization					214:223	novel ionization	208:223	novel ionization	208:223	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	19	from	improvements	142:153	arg1	modes					244:248	fragmentation modes	230:248	fragmentation modes	230:248	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	2	20	theme	new	474:476	arg1	advances					478:485	many new advances	469:485	many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization	469:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	5	21	theme	experienced	1333:1343	arg1	clinician					1345:1353	an experienced clinician	1330:1353	an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset	1330:1424	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	22	theme	applied	412:418	arg1	applications					444:455	both applied biomedical and research applications	407:455	both applied biomedical and research applications	407:455	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	0	23	theme	Spectrometry-Based	5:22	arg1	Methods					24:30	Mass Spectrometry-Based Methods	0:30	Mass Spectrometry-Based Methods for Immunoglobulin G N-Glycosylation Analysis.	0:77	Mass Spectrometry-Based Methods for Immunoglobulin G N-Glycosylation Analysis.					
34687008	2	24	from	field	494:498	arg1	terms					503:507	terms	503:507	terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization	503:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	2	25	theme	G	353:353	arg1	glycan					361:366	immunoglobulin G (IgG) glycan and glycopeptide profiling demands	338:401	glycan	361:366	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	1	26	theme	novel	208:212	arg1	ionization					214:223	novel ionization	208:223	novel ionization	208:223	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	0	27	theme	Mass	0:3	arg1	Methods					24:30	Mass Spectrometry-Based Methods	0:30	Mass Spectrometry-Based Methods for Immunoglobulin G N-Glycosylation Analysis.	0:77	Mass Spectrometry-Based Methods for Immunoglobulin G N-Glycosylation Analysis.					
34687008	1	28	theme	protein	297:303	arg1	analysis					319:326	robust and reliable protein glycosylation analysis	277:326	robust and reliable protein glycosylation analysis	277:326	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	3	29	theme	monoclonal	700:709	arg1	antibody					711:718	monoclonal antibody	700:718	monoclonal antibody	700:718	This chapter summarizes mass spectrometry basics, focusing on IgG and monoclonal antibody N-glycosylation analysis on several complexity levels.					
34687008	1	30	theme	hyphenated	105:114	arg1	spectrometry					84:95	Mass spectrometry	79:95	Mass spectrometry	79:95	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	30	theme	hyphenated	105:114	arg1	cornerstone					262:272	a cornerstone	260:272	a cornerstone of robust and reliable protein glycosylation analysis	260:326	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	30	theme	hyphenated	105:114	arg1	techniques					116:125	its hyphenated techniques	101:125	its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes	101:248	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	5	31	theme	glycoproteomic	1403:1416	arg1	dataset					1418:1424	glycoproteomic dataset	1403:1424	glycoproteomic dataset	1403:1424	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	32	from	terms	503:507	arg1	characterization					612:627	glycan structural characterization	594:627	glycan structural characterization	594:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	2	33	theme	many	469:472	arg1	advances					478:485	many new advances	469:485	many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization	469:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	1	34	theme	glycosylation	305:317	arg1	analysis					319:326	robust and reliable protein glycosylation analysis	277:326	robust and reliable protein glycosylation analysis	277:326	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	4	35	theme	excessive	997:1005	arg1	frameworks					1019:1028	excessive theoretical frameworks	997:1028	excessive theoretical frameworks	997:1028	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	5	36	theme	matrix-assisted	1152:1166	arg1	ionization					1185:1194	matrix-assisted laser desorption ionization	1152:1194	matrix-assisted laser desorption ionization	1152:1194	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	37	theme	immunoglobulin	338:351	arg1	IgG					356:358	IgG	356:358	IgG	356:358	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	2	37	theme	immunoglobulin	338:351	arg1	G					353:353	immunoglobulin G	338:353	immunoglobulin G (IgG)	338:359	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	1	38	theme	analysis	319:326	arg1	spectrometry					84:95	Mass spectrometry	79:95	Mass spectrometry	79:95	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	38	theme	analysis	319:326	arg1	cornerstone					262:272	a cornerstone	260:272	a cornerstone of robust and reliable protein glycosylation analysis	260:326	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	1	38	theme	analysis	319:326	arg1	techniques					116:125	its hyphenated techniques	101:125	its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes	101:248	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	4	39	theme	theoretical	1007:1017	arg1	frameworks					1019:1028	excessive theoretical frameworks	997:1028	excessive theoretical frameworks	997:1028	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	4	40	theme	antibody	807:814	arg1	enrichment					816:825	antibody enrichment	807:825	antibody enrichment	807:825	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	0	41	theme	G	51:51	arg1	Analysis					69:76	Immunoglobulin G N-Glycosylation Analysis	36:76	Immunoglobulin G N-Glycosylation Analysis	36:76	Mass Spectrometry-Based Methods for Immunoglobulin G N-Glycosylation Analysis.					
34687008	2	42	theme	throughput	564:573	arg1	terms					503:507	terms	503:507	terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization	503:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	2	43	theme	research	435:442	arg1	applications					444:455	both applied biomedical and research applications	407:455	both applied biomedical and research applications	407:455	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	5	44	theme	liquid	1108:1113	arg1	spectrometry					1135:1146	liquid chromatography-mass spectrometry	1108:1146	liquid chromatography-mass spectrometry	1108:1146	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	1	45	theme	fragmentation	230:242	arg1	modes					244:248	fragmentation modes	230:248	fragmentation modes	230:248	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	0	46	theme	Immunoglobulin	36:49	arg1	Analysis					69:76	Immunoglobulin G N-Glycosylation Analysis	36:76	Immunoglobulin G N-Glycosylation Analysis	36:76	Mass Spectrometry-Based Methods for Immunoglobulin G N-Glycosylation Analysis.					
34687008	5	47	theme	practical	1265:1273	arg1	point					1284:1288	a practical starting point	1263:1288	this text a practical starting point	1253:1288	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	48	gly	glycopeptide	372:383	arg2	glycopeptide					372:383	immunoglobulin G (IgG) glycan and glycopeptide profiling demands	338:401	glycopeptide	372:383	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	2	49	from	innovations	522:532	arg1	characterization					612:627	glycan structural characterization	594:627	glycan structural characterization	594:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	5	50	theme	chromatography-mass	1115:1133	arg1	spectrometry					1135:1146	liquid chromatography-mass spectrometry	1108:1146	liquid chromatography-mass spectrometry	1108:1146	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	51	theme	improved	555:562	arg1	throughput					564:573	improved throughput	555:573	improved throughput	555:573	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	5	52	from	clinician	1345:1353	arg1	field					1321:1325	the field	1317:1325	the field	1317:1325	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	5	53	theme	starting	1275:1282	arg1	point					1284:1288	a practical starting point	1263:1288	this text a practical starting point	1253:1288	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	54	theme	structural	601:610	arg1	characterization					612:627	glycan structural characterization	594:627	glycan structural characterization	594:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	2	55	from	characterization	612:627	arg1	terms					503:507	terms	503:507	terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization	503:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	5	56	theme	text	1258:1261	arg1	point					1284:1288	a practical starting point	1263:1288	this text a practical starting point	1253:1288	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	0	57	theme	N-Glycosylation	53:67	arg1	Analysis					69:76	Immunoglobulin G N-Glycosylation Analysis	36:76	Immunoglobulin G N-Glycosylation Analysis	36:76	Mass Spectrometry-Based Methods for Immunoglobulin G N-Glycosylation Analysis.					
34687008	2	58	theme	glycan	594:599	arg1	characterization					612:627	glycan structural characterization	594:627	glycan structural characterization	594:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	4	59	from	literature	977:986	arg1	examples					959:966	examples	959:966	examples	959:966	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	4	59	from	literature	977:986	arg1	breakthroughs					941:953	recent breakthroughs	934:953	recent breakthroughs	934:953	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	5	60	theme	laser	1168:1172	arg1	ionization					1185:1194	matrix-assisted laser desorption ionization	1152:1194	matrix-assisted laser desorption ionization	1152:1194	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	61	from	confidence	580:589	arg1	characterization					612:627	glycan structural characterization	594:627	glycan structural characterization	594:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	3	62	theme	N-glycosylation	720:734	arg1	analysis					736:743	N-glycosylation analysis	720:743	N-glycosylation analysis	720:743	This chapter summarizes mass spectrometry basics, focusing on IgG and monoclonal antibody N-glycosylation analysis on several complexity levels.					
34687008	3	63	theme	spectrometry	659:670	arg1	basics					672:677	mass spectrometry basics	654:677	mass spectrometry basics	654:677	This chapter summarizes mass spectrometry basics, focusing on IgG and monoclonal antibody N-glycosylation analysis on several complexity levels.					
34687008	5	64	theme	IgG	1387:1389	arg1	glycomic					1391:1398	the IgG glycomic	1383:1398	the IgG glycomic	1383:1398	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	65	from	throughput	564:573	arg1	characterization					612:627	glycan structural characterization	594:627	glycan structural characterization	594:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	2	66	theme	confidence	580:589	arg1	terms					503:507	terms	503:507	terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization	503:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	5	67	theme	desorption	1174:1183	arg1	ionization					1185:1194	matrix-assisted laser desorption ionization	1152:1194	matrix-assisted laser desorption ionization	1152:1194	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	4	68	theme	Different	775:783	arg1	labeling					844:851	labeling	844:851	labeling	844:851	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	4	68	theme	Different	775:783	arg1	enrichment					816:825	antibody enrichment	807:825	antibody enrichment	807:825	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	4	68	theme	Different	775:783	arg1	release					835:841	glycan release	828:841	glycan release	828:841	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	4	68	theme	Different	775:783	arg1	preparation					871:881	glycopeptide preparation	858:881	glycopeptide preparation	858:881	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	4	68	theme	Different	775:783	arg1	approaches					785:794	Different approaches	775:794	Different approaches	775:794	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	5	69	dep	clinician	1345:1353	arg1	trying					1355:1360	trying	1355:1360	trying to make sense out of the IgG glycomic or glycoproteomic dataset	1355:1424	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	2	70	theme	innovations	522:532	arg1	terms					503:507	terms	503:507	terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization	503:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	2	71	from	preparation	542:552	arg1	characterization					612:627	glycan structural characterization	594:627	glycan structural characterization	594:627	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	4	72	theme	glycopeptide	858:869	arg1	preparation					871:881	glycopeptide preparation	858:881	glycopeptide preparation	858:881	Different approaches, including antibody enrichment, glycan release, labeling, and glycopeptide preparation and purification, are covered and illustrated with recent breakthroughs and examples from the literature omitting excessive theoretical frameworks.					
34687008	1	73	theme	liquid	158:163	arg1	chromatography					165:178	liquid chromatography	158:178	liquid chromatography	158:178	Mass spectrometry and its hyphenated techniques enabled by the improvements in liquid chromatography, capillary electrophoresis, novel ionization, and fragmentation modes are truly a cornerstone of robust and reliable protein glycosylation analysis.					
34687008	2	74	theme	technical	512:520	arg1	innovations					522:532	technical innovations	512:532	technical innovations	512:532	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	3	75	theme	several	748:754	arg1	levels					767:772	several complexity levels	748:772	several complexity levels	748:772	This chapter summarizes mass spectrometry basics, focusing on IgG and monoclonal antibody N-glycosylation analysis on several complexity levels.					
34687008	3	76	theme	mass	654:657	arg1	basics					672:677	mass spectrometry basics	654:677	mass spectrometry basics	654:677	This chapter summarizes mass spectrometry basics, focusing on IgG and monoclonal antibody N-glycosylation analysis on several complexity levels.					
34687008	2	77	theme	profiling	385:393	arg1	demands					395:401	immunoglobulin G (IgG) glycan and glycopeptide profiling demands	338:401	demands	395:401	Boost in immunoglobulin G (IgG) glycan and glycopeptide profiling demands for both applied biomedical and research applications has brought many new advances in the field in terms of technical innovations, sample preparation, improved throughput, and confidence in glycan structural characterization.					
34687008	5	78	from	methodologies	1064:1076	arg1	ionization					1185:1194	matrix-assisted laser desorption ionization	1152:1194	matrix-assisted laser desorption ionization	1152:1194	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	5	78	from	methodologies	1064:1076	arg1	spectrometry					1135:1146	liquid chromatography-mass spectrometry	1108:1146	liquid chromatography-mass spectrometry	1108:1146	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	5	78	from	methodologies	1064:1076	arg1	glycoanalytics					1085:1098	IgG glycoanalytics	1081:1098	IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization	1081:1194	Finally, selected highly popular methodologies in IgG glycoanalytics such as liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization are discussed more thoroughly yet in simple terms making this text a practical starting point either for the beginner in the field or an experienced clinician trying to make sense out of the IgG glycomic or glycoproteomic dataset.					
34687008	3	79	dep	IgG	692:694	arg1	analysis					736:743	N-glycosylation analysis	720:743	N-glycosylation analysis	720:743	This chapter summarizes mass spectrometry basics, focusing on IgG and monoclonal antibody N-glycosylation analysis on several complexity levels.					
32663012	3	0	with	cyclization	497:507	arg1	reduction					582:590	concomitant α-iminoester reduction	557:590	concomitant α-iminoester reduction	557:590	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	3	1	theme	synthetic	342:350	arg1	sequence					352:359	the synthetic sequence	338:359	the synthetic sequence	338:359	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	2	2	from	synthesis	207:215	arg1	steps					252:256	15 steps	249:256	15 steps from d-mannose	249:271	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	2	2	from	synthesis	207:215	arg1	d-mannose					263:271	d-mannose	263:271	d-mannose	263:271	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	2	3	theme	enzymes	316:322	arg1	synthesis					207:215	the total synthesis	197:215	the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose	197:271	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	2	3	theme	enzymes	316:322	arg1	inhibitor					288:296	a potential inhibitor	276:296	a potential inhibitor of Kdo-processing enzymes	276:322	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	3	4	theme	azacyclic	663:671	arg1	ring					673:676	the Kdo-like six-membered azacyclic ring	637:676	the Kdo-like six-membered azacyclic ring	637:676	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	2	5	theme	Kdo-processing	301:314	arg1	enzymes					316:322	Kdo-processing enzymes	301:322	Kdo-processing enzymes	301:322	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	3	6	theme	concomitant	557:567	arg1	reduction					582:590	concomitant α-iminoester reduction	557:590	concomitant α-iminoester reduction	557:590	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	3	7	with	reaction	543:550	arg1	reduction					582:590	concomitant α-iminoester reduction	557:590	concomitant α-iminoester reduction	557:590	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	3	8	theme	α-stereoselective	606:622	arg1	formation					624:632	the α-stereoselective formation	602:632	the α-stereoselective formation of the Kdo-like six-membered azacyclic ring	602:676	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	3	9	theme	reductive	487:495	arg1	cyclization					497:507	a 6-exo-trig Pd-catalyzed reductive cyclization	461:507	a 6-exo-trig Pd-catalyzed reductive cyclization	461:507	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	3	10	theme	Horner-Wadsworth-Emmons	371:393	arg1	reaction					395:402	a Horner-Wadsworth-Emmons reaction	369:402	a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction	369:590	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	0	11	from	d-Mannose	50:58	arg1	Synthesis					6:14	Total Synthesis	0:14	Total Synthesis of 6-Amino-2,6-dideoxy-α-Kdo from d-Mannose	0:58	Total Synthesis of 6-Amino-2,6-dideoxy-α-Kdo from d-Mannose.					
32663012	3	12	theme	chain	423:427	arg1	homologation					429:440	the two-carbon chain homologation	408:440	the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction	408:590	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	1	13	theme	biosynthetic	103:114	arg1	pathway					116:122	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway	61:122	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway	61:122	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
32663012	1	13	theme	biosynthetic	103:114	arg1	target					139:144	a promising target	127:144	a promising target in antibacterial drug discovery	127:176	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
32663012	3	14	theme	6-exo-trig	463:472	arg1	cyclization					497:507	a 6-exo-trig Pd-catalyzed reductive cyclization	461:507	a 6-exo-trig Pd-catalyzed reductive cyclization	461:507	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	0	15	theme	Total	0:4	arg1	Synthesis					6:14	Total Synthesis	0:14	Total Synthesis of 6-Amino-2,6-dideoxy-α-Kdo from d-Mannose	0:58	Total Synthesis of 6-Amino-2,6-dideoxy-α-Kdo from d-Mannose.					
32663012	3	16	theme	ring	673:676	arg1	formation					624:632	the α-stereoselective formation	602:632	the α-stereoselective formation of the Kdo-like six-membered azacyclic ring	602:676	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	3	17	theme	Pd-catalyzed	474:485	arg1	cyclization					497:507	a 6-exo-trig Pd-catalyzed reductive cyclization	461:507	a 6-exo-trig Pd-catalyzed reductive cyclization	461:507	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	0	18	theme	6-Amino-2,6-dideoxy-α-Kdo	19:43	arg1	Synthesis					6:14	Total Synthesis	0:14	Total Synthesis of 6-Amino-2,6-dideoxy-α-Kdo from d-Mannose	0:58	Total Synthesis of 6-Amino-2,6-dideoxy-α-Kdo from d-Mannose.					
32663012	1	19	from	target	139:144	arg1	discovery					168:176	antibacterial drug discovery	149:176	antibacterial drug discovery	149:176	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
32663012	3	20	theme	Kdo-like	641:648	arg1	ring					673:676	the Kdo-like six-membered azacyclic ring	637:676	the Kdo-like six-membered azacyclic ring	637:676	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	2	21	from	d-mannose	263:271	arg1	steps					252:256	15 steps	249:256	15 steps from d-mannose	249:271	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	2	21	from	d-mannose	263:271	arg1	synthesis					207:215	the total synthesis	197:215	the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose	197:271	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	2	21	from	d-mannose	263:271	arg1	inhibitor					288:296	a potential inhibitor	276:296	a potential inhibitor of Kdo-processing enzymes	276:322	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	3	22	theme	tandem	514:519	arg1	reaction					543:550	a tandem Staudinger/aza-Wittig reaction	512:550	a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction	512:590	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	3	23	theme	two-carbon	412:421	arg1	homologation					429:440	the two-carbon chain homologation	408:440	the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction	408:590	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	3	24	theme	six-membered	650:661	arg1	ring					673:676	the Kdo-like six-membered azacyclic ring	637:676	the Kdo-like six-membered azacyclic ring	637:676	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	3	25	theme	Staudinger/aza-Wittig	521:541	arg1	reaction					543:550	a tandem Staudinger/aza-Wittig reaction	512:550	a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction	512:590	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	1	26	theme	promising	129:137	arg1	pathway					116:122	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway	61:122	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway	61:122	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
32663012	1	26	theme	promising	129:137	arg1	target					139:144	a promising target	127:144	a promising target in antibacterial drug discovery	127:176	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
32663012	2	27	theme	6-amino-2,6-dideoxy-α-Kdo	220:244	arg1	synthesis					207:215	the total synthesis	197:215	the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose	197:271	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	2	27	theme	6-amino-2,6-dideoxy-α-Kdo	220:244	arg1	inhibitor					288:296	a potential inhibitor	276:296	a potential inhibitor of Kdo-processing enzymes	276:322	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	3	28	theme	α-iminoester	569:580	arg1	reduction					582:590	concomitant α-iminoester reduction	557:590	concomitant α-iminoester reduction	557:590	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	2	29	theme	potential	278:286	arg1	synthesis					207:215	the total synthesis	197:215	the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose	197:271	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	2	29	theme	potential	278:286	arg1	inhibitor					288:296	a potential inhibitor	276:296	a potential inhibitor of Kdo-processing enzymes	276:322	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	3	30	theme	Key	325:327	arg1	steps					329:333	Key steps	325:333	Key steps of the synthetic sequence	325:359	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	1	31	theme	acid	92:95	arg1	pathway					116:122	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway	61:122	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway	61:122	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
32663012	1	31	theme	acid	92:95	arg1	target					139:144	a promising target	127:144	a promising target in antibacterial drug discovery	127:176	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
32663012	1	32	theme	antibacterial	149:161	arg1	discovery					168:176	antibacterial drug discovery	149:176	antibacterial drug discovery	149:176	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
32663012	3	33	theme	sequence	352:359	arg1	steps					329:333	Key steps	325:333	Key steps of the synthetic sequence	325:359	Key steps of the synthetic sequence involve a Horner-Wadsworth-Emmons reaction for the two-carbon chain homologation followed by either a 6-exo-trig Pd-catalyzed reductive cyclization or a tandem Staudinger/aza-Wittig reaction with concomitant α-iminoester reduction, enabling the α-stereoselective formation of the Kdo-like six-membered azacyclic ring.					
32663012	2	34	theme	total	201:205	arg1	synthesis					207:215	the total synthesis	197:215	the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose	197:271	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	2	34	theme	total	201:205	arg1	inhibitor					288:296	a potential inhibitor	276:296	a potential inhibitor of Kdo-processing enzymes	276:322	Herein, we report the total synthesis of 6-amino-2,6-dideoxy-α-Kdo in 15 steps from d-mannose as a potential inhibitor of Kdo-processing enzymes.					
32663012	1	35	theme	drug	163:166	arg1	discovery					168:176	antibacterial drug discovery	149:176	antibacterial drug discovery	149:176	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
32663012	1	36	theme	3-Deoxy-d-manno-oct-2-ulosonic	61:90	arg1	Kdo					98:100	Kdo	98:100	Kdo	98:100	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
32663012	1	36	theme	3-Deoxy-d-manno-oct-2-ulosonic	61:90	arg1	acid					92:95	3-Deoxy-d-manno-oct-2-ulosonic acid	61:95	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway	61:122	3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) biosynthetic pathway is a promising target in antibacterial drug discovery.					
34710380	6	0	theme	antibody	1284:1291	arg1	structure					1267:1275	the overall 3D structure	1252:1275	the overall 3D structure of the antibody	1252:1291	Trajectory analysis showed different dynamical behaviors and pointed out that sugars can influence the overall 3D structure of the antibody.					
34710380	8	1	theme	asymmetric	1646:1655	arg1	behavior					1657:1664	an asymmetric behavior	1643:1664	an asymmetric behavior of Fab portions shown by all the simulated biopolymers	1643:1719	As secondary evidence, we observed a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers, making the dynamical asymmetry a new, to our knowledge, molecular aspect that may be further investigated.					
34710380	0	2	theme	molecular	74:82	arg1	dynamics					84:91	molecular dynamics	74:91	molecular dynamics	74:91	IgG1 conformational behavior: elucidation of the N-glycosylation role via molecular dynamics.					
34710380	1	3	theme	used	147:150	arg1	biopharmaceuticals					152:169	the most used biopharmaceuticals	138:169	the most used biopharmaceuticals for human therapy	138:187	Currently, monoclonal antibodies (mAbs) are the most used biopharmaceuticals for human therapy.					
34710380	1	3	theme	used	147:150	arg1	antibodies					116:125	monoclonal antibodies	105:125	monoclonal antibodies (mAbs)	105:132	Currently, monoclonal antibodies (mAbs) are the most used biopharmaceuticals for human therapy.					
34710380	8	4	theme	Fab	1669:1671	arg1	portions					1673:1680	Fab portions	1669:1680	Fab portions shown by all the simulated biopolymers	1669:1719	As secondary evidence, we observed a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers, making the dynamical asymmetry a new, to our knowledge, molecular aspect that may be further investigated.					
34710380	5	5	theme	dynamics	1034:1041	arg1	simulations					1043:1053	molecular dynamics simulations	1024:1053	molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody	1024:1150	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	7	6	theme	Fc	1462:1463	arg1	domain					1465:1470	the Fc domain	1458:1470	the Fc domain	1458:1470	As a result, we propose a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor.					
34710380	9	7	from	contribution	1883:1894	arg1	architecture					1924:1935	the structural architecture	1909:1935	the structural architecture of mAbs	1909:1943	In conclusion, these findings can help understand the contribution of sugars on the structural architecture of mAbs, paving the way to novel strategies of pharmaceutical development.					
34710380	6	8	theme	different	1180:1188	arg1	behaviors					1200:1208	different dynamical behaviors	1180:1208	different dynamical behaviors	1180:1208	Trajectory analysis showed different dynamical behaviors and pointed out that sugars can influence the overall 3D structure of the antibody.					
34710380	4	9	theme	structural	713:722	arg1	role					724:727	the structural role	709:727	the structural role exhibited by glycans, particularly fucose, in mAb conformational freedom that can potentially affect the receptor recognition	709:853	In this work, we try to elucidate via in silico procedures the structural role exhibited by glycans, particularly fucose, in mAb conformational freedom that can potentially affect the receptor recognition.					
34710380	9	10	theme	novel	1964:1968	arg1	strategies					1970:1979	novel strategies	1964:1979	novel strategies of pharmaceutical development	1964:2009	In conclusion, these findings can help understand the contribution of sugars on the structural architecture of mAbs, paving the way to novel strategies of pharmaceutical development.					
34710380	2	11	theme	functions	261:269	arg1	One					190:192	One	190:192	One	190:192	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	11	theme	functions	261:269	arg1	aspects					205:211	the key aspects	197:211	the key aspects in their development	197:232	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	11	theme	functions	261:269	arg1	control					241:247	the control	237:247	the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics	237:417	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	12	from	aspects	205:211	arg1	development					222:232	their development	216:232	their development	216:232	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	4	13	theme	in silico	688:696	arg1	procedures					698:707	in silico procedures	688:707	in silico procedures	688:707	In this work, we try to elucidate via in silico procedures the structural role exhibited by glycans, particularly fucose, in mAb conformational freedom that can potentially affect the receptor recognition.					
34710380	5	14	theme	glycosylated	1076:1087	arg1	species					1089:1095	three differently glycosylated species	1058:1095	three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody	1058:1150	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	6	15	theme	3D	1264:1265	arg1	structure					1267:1275	the overall 3D structure	1252:1275	the overall 3D structure of the antibody	1252:1291	Trajectory analysis showed different dynamical behaviors and pointed out that sugars can influence the overall 3D structure of the antibody.					
34710380	2	16	theme	effector	252:259	arg1	functions					261:269	effector functions	252:269	effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics	252:417	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	5	17	gly	aglycosylated	1098:1110	arg1	species					1089:1095	three differently glycosylated species	1058:1095	three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody	1058:1150	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	8	18	theme	antibodies	1608:1617	arg1	flexibility					1589:1599	a high flexibility	1582:1599	a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers	1582:1719	As secondary evidence, we observed a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers, making the dynamical asymmetry a new, to our knowledge, molecular aspect that may be further investigated.					
34710380	5	19	theme	species	1089:1095	arg1	simulations					1043:1053	molecular dynamics simulations	1024:1053	molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody	1024:1150	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	6	20	theme	overall	1256:1262	arg1	structure					1267:1275	the overall 3D structure	1252:1275	the overall 3D structure of the antibody	1252:1291	Trajectory analysis showed different dynamical behaviors and pointed out that sugars can influence the overall 3D structure of the antibody.					
34710380	3	21	from	portion	446:452	arg1	N-glycosylation					420:434	N-glycosylation	420:434	N-glycosylation at the Fc portion	420:452	N-glycosylation at the Fc portion can regulate these mechanisms, and much experimental evidence suggests that modifications of glycosidic chains can affect antibody binding to FcγRIIIa, consequently impacting the immune response.					
34710380	0	22	theme	IgG1	0:3	arg1	behavior					20:27	IgG1 conformational behavior	0:27	IgG1 conformational behavior: elucidation of the N-glycosylation role via molecular dynamics.	0:92	IgG1 conformational behavior: elucidation of the N-glycosylation role via molecular dynamics.					
34710380	7	23	theme	conformational	1393:1406	arg1	constraints					1408:1418	conformational constraints	1393:1418	conformational constraints	1393:1418	As a result, we propose a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor.					
34710380	0	24	theme	conformational	5:18	arg1	behavior					20:27	IgG1 conformational behavior	0:27	IgG1 conformational behavior: elucidation of the N-glycosylation role via molecular dynamics.	0:92	IgG1 conformational behavior: elucidation of the N-glycosylation role via molecular dynamics.					
34710380	3	25	theme	much	489:492	arg1	evidence					507:514	much experimental evidence	489:514	much experimental evidence	489:514	N-glycosylation at the Fc portion can regulate these mechanisms, and much experimental evidence suggests that modifications of glycosidic chains can affect antibody binding to FcγRIIIa, consequently impacting the immune response.					
34710380	4	26	theme	receptor	834:841	arg1	recognition					843:853	the receptor recognition	830:853	the receptor recognition	830:853	In this work, we try to elucidate via in silico procedures the structural role exhibited by glycans, particularly fucose, in mAb conformational freedom that can potentially affect the receptor recognition.					
34710380	9	27	theme	pharmaceutical	1984:1997	arg1	development					1999:2009	pharmaceutical development	1984:2009	pharmaceutical development	1984:2009	In conclusion, these findings can help understand the contribution of sugars on the structural architecture of mAbs, paving the way to novel strategies of pharmaceutical development.					
34710380	3	28	theme	glycosidic	547:556	arg1	chains					558:563	glycosidic chains	547:563	glycosidic chains	547:563	N-glycosylation at the Fc portion can regulate these mechanisms, and much experimental evidence suggests that modifications of glycosidic chains can affect antibody binding to FcγRIIIa, consequently impacting the immune response.					
34710380	8	29	theme	high	1584:1587	arg1	flexibility					1589:1599	a high flexibility	1582:1599	a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers	1582:1719	As secondary evidence, we observed a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers, making the dynamical asymmetry a new, to our knowledge, molecular aspect that may be further investigated.					
34710380	1	30	theme	human	175:179	arg1	therapy					181:187	human therapy	175:187	human therapy	175:187	Currently, monoclonal antibodies (mAbs) are the most used biopharmaceuticals for human therapy.					
34710380	2	31	theme	biotherapeutics	403:417	arg1	action					393:398	the action	389:398	the action of biotherapeutics	389:417	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	7	32	theme	whole	1427:1431	arg1	antibody					1433:1440	the whole antibody	1423:1440	the whole antibody	1423:1440	As a result, we propose a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor.					
34710380	8	33	theme	dynamical	1733:1741	arg1	asymmetry					1743:1751	the dynamical asymmetry	1729:1751	the dynamical asymmetry	1729:1751	As secondary evidence, we observed a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers, making the dynamical asymmetry a new, to our knowledge, molecular aspect that may be further investigated.					
34710380	9	34	theme	development	1999:2009	arg1	strategies					1970:1979	novel strategies	1964:1979	novel strategies of pharmaceutical development	1964:2009	In conclusion, these findings can help understand the contribution of sugars on the structural architecture of mAbs, paving the way to novel strategies of pharmaceutical development.					
34710380	3	35	theme	chains	558:563	arg1	modifications					530:542	modifications	530:542	modifications of glycosidic chains	530:563	N-glycosylation at the Fc portion can regulate these mechanisms, and much experimental evidence suggests that modifications of glycosidic chains can affect antibody binding to FcγRIIIa, consequently impacting the immune response.					
34710380	5	36	gly	glycosylated	1076:1087	arg1	species					1089:1095	three differently glycosylated species	1058:1095	three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody	1058:1150	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	5	37	theme	marketed	879:886	arg1	adalimumab					865:874	adalimumab	865:874	adalimumab	865:874	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	5	37	theme	marketed	879:886	arg1	IgG1					888:891	a marketed IgG1	877:891	a marketed IgG1	877:891	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	7	38	theme	putative	1320:1327	arg1	mechanism					1340:1348	a putative structural mechanism	1318:1348	a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor	1318:1544	As a result, we propose a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor.					
34710380	7	38	theme	putative	1320:1327	arg1	result					1299:1304	a result	1297:1304	a result	1297:1304	As a result, we propose a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor.					
34710380	6	39	dep	showed	1173:1178	arg1	pointed					1214:1220	pointed	1214:1220	pointed out that sugars can influence the overall 3D structure of the antibody	1214:1291	Trajectory analysis showed different dynamical behaviors and pointed out that sugars can influence the overall 3D structure of the antibody.					
34710380	2	40	theme	key	201:203	arg1	aspects					205:211	the key aspects	197:211	the key aspects in their development	197:232	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	7	41	theme	fucose	1375:1380	arg1	presence					1363:1370	the presence	1359:1370	the presence of fucose	1359:1380	As a result, we propose a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor.					
34710380	5	42	theme	homology	979:986	arg1	approaches					997:1006	homology modeling approaches	979:1006	homology modeling approaches	979:1006	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	2	43	theme	action	393:398	arg1	mechanism					376:384	a secondary mechanism	364:384	a secondary mechanism of the action of biotherapeutics	364:417	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	43	theme	action	393:398	arg1	Fc					332:333	fragment crystallizable (Fc) and Fcγ receptors	307:352	Fc	332:333	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	43	theme	action	393:398	arg1	receptors					344:352	fragment crystallizable (Fc) and Fcγ receptors	307:352	receptors	344:352	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	3	44	theme	experimental	494:505	arg1	evidence					507:514	much experimental evidence	489:514	much experimental evidence	489:514	N-glycosylation at the Fc portion can regulate these mechanisms, and much experimental evidence suggests that modifications of glycosidic chains can affect antibody binding to FcγRIIIa, consequently impacting the immune response.					
34710380	3	45	theme	immune	633:638	arg1	response					640:647	the immune response	629:647	the immune response	629:647	N-glycosylation at the Fc portion can regulate these mechanisms, and much experimental evidence suggests that modifications of glycosidic chains can affect antibody binding to FcγRIIIa, consequently impacting the immune response.					
34710380	1	46	theme	monoclonal	105:114	arg1	mAbs					128:131	mAbs	128:131	mAbs	128:131	Currently, monoclonal antibodies (mAbs) are the most used biopharmaceuticals for human therapy.					
34710380	1	46	theme	monoclonal	105:114	arg1	antibodies					116:125	monoclonal antibodies	105:125	monoclonal antibodies (mAbs)	105:132	Currently, monoclonal antibodies (mAbs) are the most used biopharmaceuticals for human therapy.					
34710380	1	46	theme	monoclonal	105:114	arg1	biopharmaceuticals					152:169	the most used biopharmaceuticals	138:169	the most used biopharmaceuticals for human therapy	138:187	Currently, monoclonal antibodies (mAbs) are the most used biopharmaceuticals for human therapy.					
34710380	7	47	with	interaction	1516:1526	arg1	receptor					1537:1544	the receptor	1533:1544	the receptor	1533:1544	As a result, we propose a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor.					
34710380	3	48	theme	Fc	443:444	arg1	portion					446:452	the Fc portion	439:452	the Fc portion	439:452	N-glycosylation at the Fc portion can regulate these mechanisms, and much experimental evidence suggests that modifications of glycosidic chains can affect antibody binding to FcγRIIIa, consequently impacting the immune response.					
34710380	8	49	theme	portions	1673:1680	arg1	behavior					1657:1664	an asymmetric behavior	1643:1664	an asymmetric behavior of Fab portions shown by all the simulated biopolymers	1643:1719	As secondary evidence, we observed a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers, making the dynamical asymmetry a new, to our knowledge, molecular aspect that may be further investigated.					
34710380	9	50	theme	sugars	1899:1904	arg1	contribution					1883:1894	the contribution	1879:1894	the contribution of sugars on the structural architecture of mAbs	1879:1943	In conclusion, these findings can help understand the contribution of sugars on the structural architecture of mAbs, paving the way to novel strategies of pharmaceutical development.					
34710380	3	51	theme	antibody	576:583	arg1	binding					585:591	antibody binding	576:591	antibody binding to FcγRIIIa	576:603	N-glycosylation at the Fc portion can regulate these mechanisms, and much experimental evidence suggests that modifications of glycosidic chains can affect antibody binding to FcγRIIIa, consequently impacting the immune response.					
34710380	8	52	theme	new	1755:1757	arg1	aspect					1788:1793	a new, to our knowledge, molecular aspect	1753:1793	a new, to our knowledge, molecular aspect that may be further investigated	1753:1826	As secondary evidence, we observed a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers, making the dynamical asymmetry a new, to our knowledge, molecular aspect that may be further investigated.					
34710380	5	53	dep	species	1089:1095	arg1	antibody					1143:1150	fucosylated antibody	1131:1150	fucosylated antibody	1131:1150	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	5	53	dep	species	1089:1095	arg1	afucosylated					1113:1124	afucosylated	1113:1124	afucosylated	1113:1124	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	5	53	dep	species	1089:1095	arg1	aglycosylated					1098:1110	aglycosylated	1098:1110	aglycosylated	1098:1110	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	4	54	theme	conformational	779:792	arg1	freedom					794:800	mAb conformational freedom	775:800	mAb conformational freedom that can potentially affect the receptor recognition	775:853	In this work, we try to elucidate via in silico procedures the structural role exhibited by glycans, particularly fucose, in mAb conformational freedom that can potentially affect the receptor recognition.					
34710380	2	55	theme	secondary	366:374	arg1	mechanism					376:384	a secondary mechanism	364:384	a secondary mechanism of the action of biotherapeutics	364:417	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	55	theme	secondary	366:374	arg1	Fc					332:333	fragment crystallizable (Fc) and Fcγ receptors	307:352	Fc	332:333	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	55	theme	secondary	366:374	arg1	receptors					344:352	fragment crystallizable (Fc) and Fcγ receptors	307:352	receptors	344:352	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	0	56	theme	N-glycosylation	49:63	arg1	role					65:68	the N-glycosylation role	45:68	the N-glycosylation role	45:68	IgG1 conformational behavior: elucidation of the N-glycosylation role via molecular dynamics.					
34710380	5	57	theme	fucosylated	1131:1141	arg1	antibody					1143:1150	fucosylated antibody	1131:1150	fucosylated antibody	1131:1150	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	5	58	theme	three-dimensional	938:954	arg1	structure					961:969	its three-dimensional (3D) structure	934:969	its three-dimensional (3D) structure	934:969	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	2	59	theme	aspects	205:211	arg1	One					190:192	One	190:192	One	190:192	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	59	theme	aspects	205:211	arg1	aspects					205:211	the key aspects	197:211	the key aspects in their development	197:232	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	59	theme	aspects	205:211	arg1	control					241:247	the control	237:247	the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics	237:417	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	9	60	theme	structural	1913:1922	arg1	architecture					1924:1935	the structural architecture	1909:1935	the structural architecture of mAbs	1909:1943	In conclusion, these findings can help understand the contribution of sugars on the structural architecture of mAbs, paving the way to novel strategies of pharmaceutical development.					
34710380	5	61	dep	three-dimensional	938:954	arg1	3D					957:958	3D	957:958	3D	957:958	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	3	62	gly	N-glycosylation	420:434	arg2	portion					446:452	the Fc portion	439:452	the Fc portion	439:452	N-glycosylation at the Fc portion can regulate these mechanisms, and much experimental evidence suggests that modifications of glycosidic chains can affect antibody binding to FcγRIIIa, consequently impacting the immune response.					
34710380	3	62	gly	N-glycosylation	420:434	arg1	portion					446:452	the Fc portion	439:452	the Fc portion	439:452	N-glycosylation at the Fc portion can regulate these mechanisms, and much experimental evidence suggests that modifications of glycosidic chains can affect antibody binding to FcγRIIIa, consequently impacting the immune response.					
34710380	7	63	theme	structural	1329:1338	arg1	mechanism					1340:1348	a putative structural mechanism	1318:1348	a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor	1318:1544	As a result, we propose a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor.					
34710380	7	63	theme	structural	1329:1338	arg1	result					1299:1304	a result	1297:1304	a result	1297:1304	As a result, we propose a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor.					
34710380	5	64	theme	general	899:905	arg1	adalimumab					865:874	adalimumab	865:874	adalimumab	865:874	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	5	64	theme	general	899:905	arg1	template					907:914	a general template	897:914	a general template	897:914	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	7	65	theme	suitable	1499:1506	arg1	conformation					1486:1497	a conformation	1484:1497	a conformation suitable for the interaction with the receptor	1484:1544	As a result, we propose a putative structural mechanism by which the presence of fucose introduces conformational constraints in the whole antibody and not only in the Fc domain, preventing a conformation suitable for the interaction with the receptor.					
34710380	5	66	theme	modeling	988:995	arg1	approaches					997:1006	homology modeling approaches	979:1006	homology modeling approaches	979:1006	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	2	67	theme	crystallizable	316:329	arg1	mechanism					376:384	a secondary mechanism	364:384	a secondary mechanism of the action of biotherapeutics	364:417	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	67	theme	crystallizable	316:329	arg1	Fc					332:333	fragment crystallizable (Fc) and Fcγ receptors	307:352	Fc	332:333	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	67	theme	crystallizable	316:329	arg1	receptors					344:352	fragment crystallizable (Fc) and Fcγ receptors	307:352	receptors	344:352	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	9	68	theme	mAbs	1940:1943	arg1	architecture					1924:1935	the structural architecture	1909:1935	the structural architecture of mAbs	1909:1943	In conclusion, these findings can help understand the contribution of sugars on the structural architecture of mAbs, paving the way to novel strategies of pharmaceutical development.					
34710380	6	69	theme	Trajectory	1153:1162	arg1	analysis					1164:1171	Trajectory analysis	1153:1171	Trajectory analysis	1153:1171	Trajectory analysis showed different dynamical behaviors and pointed out that sugars can influence the overall 3D structure of the antibody.					
34710380	0	70	dep	behavior	20:27	arg1	elucidation					30:40	elucidation	30:40	IgG1 conformational behavior: elucidation of the N-glycosylation role via molecular dynamics.	0:92	IgG1 conformational behavior: elucidation of the N-glycosylation role via molecular dynamics.					
34710380	5	71	gly	fucosylated	1131:1141	arg1	antibody					1143:1150	fucosylated antibody	1131:1150	fucosylated antibody	1131:1150	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	2	72	theme	fragment	307:314	arg1	mechanism					376:384	a secondary mechanism	364:384	a secondary mechanism of the action of biotherapeutics	364:417	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	72	theme	fragment	307:314	arg1	Fc					332:333	fragment crystallizable (Fc) and Fcγ receptors	307:352	Fc	332:333	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	72	theme	fragment	307:314	arg1	receptors					344:352	fragment crystallizable (Fc) and Fcγ receptors	307:352	receptors	344:352	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	8	73	dep	new	1755:1757	arg1	molecular					1778:1786	molecular	1778:1786	molecular	1778:1786	As secondary evidence, we observed a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers, making the dynamical asymmetry a new, to our knowledge, molecular aspect that may be further investigated.					
34710380	6	74	dep	pointed	1214:1220	arg1	out					1222:1224	out	1222:1224	out	1222:1224	Trajectory analysis showed different dynamical behaviors and pointed out that sugars can influence the overall 3D structure of the antibody.					
34710380	5	75	theme	molecular	1024:1032	arg1	simulations					1043:1053	molecular dynamics simulations	1024:1053	molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody	1024:1150	By using adalimumab, a marketed IgG1, as a general template, after rebuilding its three-dimensional (3D) structure through homology modeling approaches, we carried out molecular dynamics simulations of three differently glycosylated species: aglycosylated, afucosylated, and fucosylated antibody.					
34710380	4	76	theme	mAb	775:777	arg1	freedom					794:800	mAb conformational freedom	775:800	mAb conformational freedom that can potentially affect the receptor recognition	775:853	In this work, we try to elucidate via in silico procedures the structural role exhibited by glycans, particularly fucose, in mAb conformational freedom that can potentially affect the receptor recognition.					
34710380	8	77	theme	simulated	1699:1707	arg1	biopolymers					1709:1719	all the simulated biopolymers	1691:1719	all the simulated biopolymers	1691:1719	As secondary evidence, we observed a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers, making the dynamical asymmetry a new, to our knowledge, molecular aspect that may be further investigated.					
34710380	0	78	theme	role	65:68	arg1	elucidation					30:40	elucidation	30:40	IgG1 conformational behavior: elucidation of the N-glycosylation role via molecular dynamics.	0:92	IgG1 conformational behavior: elucidation of the N-glycosylation role via molecular dynamics.					
34710380	6	79	theme	dynamical	1190:1198	arg1	behaviors					1200:1208	different dynamical behaviors	1180:1208	different dynamical behaviors	1180:1208	Trajectory analysis showed different dynamical behaviors and pointed out that sugars can influence the overall 3D structure of the antibody.					
34710380	2	80	theme	Fcγ	340:342	arg1	mechanism					376:384	a secondary mechanism	364:384	a secondary mechanism of the action of biotherapeutics	364:417	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	80	theme	Fcγ	340:342	arg1	Fc					332:333	fragment crystallizable (Fc) and Fcγ receptors	307:352	Fc	332:333	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	2	80	theme	Fcγ	340:342	arg1	receptors					344:352	fragment crystallizable (Fc) and Fcγ receptors	307:352	receptors	344:352	One of the key aspects in their development is the control of effector functions mediated by the interaction between fragment crystallizable (Fc) and Fcγ receptors, which is a secondary mechanism of the action of biotherapeutics.					
34710380	8	81	theme	secondary	1550:1558	arg1	evidence					1560:1567	secondary evidence	1550:1567	secondary evidence	1550:1567	As secondary evidence, we observed a high flexibility of the antibodies that is translated into an asymmetric behavior of Fab portions shown by all the simulated biopolymers, making the dynamical asymmetry a new, to our knowledge, molecular aspect that may be further investigated.					
34132788	1	0	theme	inflammatory	359:370	arg1	biomarkers					372:381	inflammatory biomarkers	359:381	inflammatory biomarkers	359:381	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	10	1	theme	N-glycosylation	2067:2081	arg1	profile					2083:2089	less inflammatory N-glycosylation profile	2049:2089	less inflammatory N-glycosylation profile	2049:2089	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	1	2	theme	healthy	392:398	arg1	N = 33					413:418	N = 33	413:418	N = 33	413:418	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	1	2	theme	healthy	392:398	arg1	individuals					400:410	healthy individuals	392:410	healthy individuals (N = 33)	392:419	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	8	3	dep	decreased	1617:1625	arg1	whereas					1628:1634	whereas	1628:1634	whereas	1628:1634	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	2	4	theme	249 mg	572:577	arg1	PUFAs/day					583:591	249 mg n-3 PUFAs/day	572:591	249 mg n-3 PUFAs/day	572:591	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	2	4	theme	249 mg	572:577	arg1	eggs/daily					560:569	[consumed three regular hens' eggs/daily	530:569	[consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)]	530:593	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	6	5	theme	Healthy	1158:1164	arg1	subgroups					1166:1174	both Healthy subgroups	1153:1174	both Healthy subgroups	1153:1174	IL-10 significantly increased in both Healthy subgroups, whereas no change occurred in CV subgroups.					
34132788	10	6	theme	IgG	1914:1916	arg1	traits					1927:1932	IgG N-glycan traits	1914:1932	IgG N-glycan traits	1914:1932	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	8	7	dep	traits	1469:1474	arg1	decreased					1617:1625	decreased	1617:1625	decreased	1617:1625	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	10	8	theme	healthy	1947:1953	arg1	individuals					1955:1965	healthy individuals	1947:1965	healthy individuals	1947:1965	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	7	9	theme	core	1364:1367	arg1	CF					1383:1384	CF	1383:1384	CF	1383:1384	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.					
34132788	7	9	theme	core	1364:1367	arg1	fucosylation					1369:1380	core fucosylation	1364:1380	core fucosylation (CF)	1364:1385	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.					
34132788	4	10	theme	Total	887:891	arg1	protein					900:906	Total plasma protein	887:906	Total plasma protein	887:906	Total plasma protein and IgG N-glycome have been profiled before and after dietary protocols.					
34132788	6	11	theme	CV	1207:1208	arg1	subgroups					1210:1218	CV subgroups	1207:1218	CV subgroups	1207:1218	IL-10 significantly increased in both Healthy subgroups, whereas no change occurred in CV subgroups.					
34132788	3	12	theme	fatty	744:748	arg1	10					823:824	10	823:824	10	823:824	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	12	theme	fatty	744:748	arg1	protein					796:802	high-sensitivity C-reactive protein	768:802	high-sensitivity C-reactive protein	768:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	12	theme	fatty	744:748	arg1	acids					750:754	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	12	theme	fatty	744:748	arg1	alpha					866:870	tumor necrosis factor alpha	844:870	tumor necrosis factor alpha	844:870	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	12	theme	fatty	744:748	arg1	interleukin					805:815	interleukin 6 and 10	805:824	interleukin	805:815	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	12	theme	fatty	744:748	arg1	profile					756:762	profile	756:762	profile	756:762	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	0	13	theme	healthy	127:133	arg1	individuals					135:145	healthy individuals	127:145	healthy individuals	127:145	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	5	14	theme	PUFAs	1049:1053	arg1	eggs					1059:1062	n-3 PUFAs hen eggs	1045:1062	n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV	1045:1117	Serum n-3 PUFAs concentration significantly increased following n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV.					
34132788	1	15	theme	polyunsaturated	216:230	arg1	acids					238:242	n-3 polyunsaturated fatty acids	212:242	n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption	212:284	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	1	15	theme	polyunsaturated	216:230	arg1	PUFAs					249:253	n-3 PUFAs	245:253	n-3 PUFAs	245:253	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	10	16	from	traits	1927:1932	arg1	patients					2015:2022	CV patients	2012:2022	CV patients	2012:2022	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	10	16	from	traits	1927:1932	arg1	individuals					1955:1965	healthy individuals	1947:1965	healthy individuals	1947:1965	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	8	17	theme	Derived	1431:1437	arg1	traits					1469:1474	Derived total plasma protein N-glycan traits	1431:1474	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.	1431:1761	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	2	18	theme	hen	680:682	arg1	eggs/daily					684:693	three n-3 PUFAs-enriched hen eggs/daily	655:693	three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks	655:730	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	2	18	theme	hen	680:682	arg1	PUFAs/day					708:716	1053 mg n-3 PUFAs/day	696:716	1053 mg n-3 PUFAs/day	696:716	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	5	19	theme	PUFAs	991:995	arg1	concentration					997:1009	Serum n-3 PUFAs concentration	981:1009	Serum n-3 PUFAs concentration	981:1009	Serum n-3 PUFAs concentration significantly increased following n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV.					
34132788	1	20	from	effect	202:207	arg1	level					383:387	immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level	289:387	immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level	289:387	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	8	21	theme	plasma	1445:1450	arg1	traits					1469:1474	Derived total plasma protein N-glycan traits	1431:1474	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.	1431:1761	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	2	22	theme	n-3	661:663	arg1	eggs/daily					684:693	three n-3 PUFAs-enriched hen eggs/daily	655:693	three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks	655:730	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	2	22	theme	n-3	661:663	arg1	PUFAs/day					708:716	1053 mg n-3 PUFAs/day	696:716	1053 mg n-3 PUFAs/day	696:716	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	3	23	theme	high-sensitivity	768:783	arg1	protein					796:802	high-sensitivity C-reactive protein	768:802	high-sensitivity C-reactive protein	768:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	23	theme	high-sensitivity	768:783	arg1	acids					750:754	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	24	dep	interleukin	805:815	arg1	IL-10					833:837	IL-10	833:837	IL-10	833:837	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	24	dep	interleukin	805:815	arg1	IL-6					827:830	IL-6	827:830	IL-6	827:830	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	8	25	theme	N-glycan	1460:1467	arg1	traits					1469:1474	Derived total plasma protein N-glycan traits	1431:1474	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.	1431:1761	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	2	26	theme	n-3	704:706	arg1	eggs/daily					684:693	three n-3 PUFAs-enriched hen eggs/daily	655:693	three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks	655:730	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	2	26	theme	n-3	704:706	arg1	PUFAs/day					708:716	1053 mg n-3 PUFAs/day	696:716	1053 mg n-3 PUFAs/day	696:716	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	10	27	theme	protein	1985:1991	arg1	traits					2002:2007	total plasma protein N-glycan traits	1972:2007	total plasma protein N-glycan traits in CV patients	1972:2022	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	0	28	theme	eggs	59:62	arg1	consumption					64:74	n-3 polyunsaturated fatty acids-enriched hen eggs consumption	14:74	n-3 polyunsaturated fatty acids-enriched hen eggs consumption	14:74	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	5	29	theme	n-3	1084:1086	arg1	PUFAs-Healthy					1088:1100	n-3 PUFAs-Healthy	1084:1100	n-3 PUFAs-Healthy	1084:1100	Serum n-3 PUFAs concentration significantly increased following n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV.					
34132788	1	30	theme	plasma	322:327	arg1	profiles					346:353	total plasma protein N-glycan profiles	316:353	total plasma protein N-glycan profiles	316:353	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	8	31	theme	branching	1482:1490	arg1	trigalactosylation					1501:1518	trigalactosylation	1501:1518	trigalactosylation	1501:1518	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	31	theme	branching	1482:1490	arg1	tetragalactosylation					1521:1540	tetragalactosylation	1521:1540	tetragalactosylation	1521:1540	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	31	theme	branching	1482:1490	arg1	fucosylation					1590:1601	antennary fucosylation	1580:1601	antennary fucosylation	1580:1601	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	31	theme	branching	1482:1490	arg1	glycans					1492:1498	high branching glycans	1477:1498	high branching glycans	1477:1498	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	31	theme	branching	1482:1490	arg1	trisialylation					1543:1556	trisialylation	1543:1556	trisialylation	1543:1556	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	31	theme	branching	1482:1490	arg1	tetrasialylation					1559:1574	tetrasialylation	1559:1574	tetrasialylation	1559:1574	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	10	32	theme	CV	2012:2013	arg1	patients					2015:2022	CV patients	2012:2022	CV patients	2012:2022	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	5	33	theme	eggs	1059:1062	arg1	consumption					1064:1074	n-3 PUFAs hen eggs consumption	1045:1074	n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV	1045:1117	Serum n-3 PUFAs concentration significantly increased following n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV.					
34132788	7	34	dep	traits	1242:1247	arg1	decreased					1298:1306	decreased	1298:1306	decreased	1298:1306	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.					
34132788	3	35	theme	factor	859:864	arg1	alpha					866:870	tumor necrosis factor alpha	844:870	tumor necrosis factor alpha	844:870	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	35	theme	factor	859:864	arg1	acids					750:754	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	4	36	theme	IgG	912:914	arg1	N-glycome					916:924	IgG N-glycome	912:924	IgG N-glycome	912:924	Total plasma protein and IgG N-glycome have been profiled before and after dietary protocols.					
34132788	2	37	theme	n-3	600:602	arg1	PUFAs-Healthy					604:616	n-3 PUFAs-Healthy	600:616	n-3 PUFAs-Healthy	600:616	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	0	38	theme	plasma	93:98	arg1	N-glycosylation					108:122	total plasma protein N-glycosylation	87:122	total plasma protein N-glycosylation	87:122	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	10	39	theme	N-glycan	1993:2000	arg1	traits					2002:2007	total plasma protein N-glycan traits	1972:2007	total plasma protein N-glycan traits in CV patients	1972:2022	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	3	40	theme	tumor	844:848	arg1	alpha					866:870	tumor necrosis factor alpha	844:870	tumor necrosis factor alpha	844:870	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	40	theme	tumor	844:848	arg1	acids					750:754	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	7	41	theme	n-3	1311:1313	arg1	PUFAs-Healthy					1315:1327	n-3 PUFAs-Healthy	1311:1327	n-3 PUFAs-Healthy	1311:1327	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.					
34132788	1	42	theme	eggs	269:272	arg1	consumption					274:284	n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption	212:284	n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption	212:284	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	2	43	theme	PUFAs-CV	626:633	arg1	subgroups					635:643	n-3 PUFAs-CV subgroups	622:643	n-3 PUFAs-CV subgroups	622:643	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	5	44	from	consumption	1064:1074	arg1	PUFAs-Healthy					1088:1100	n-3 PUFAs-Healthy	1084:1100	n-3 PUFAs-Healthy	1084:1100	Serum n-3 PUFAs concentration significantly increased following n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV.					
34132788	5	44	from	consumption	1064:1074	arg1	PUFAs-CV					1110:1117	n-3 PUFAs-CV	1106:1117	n-3 PUFAs-CV	1106:1117	Serum n-3 PUFAs concentration significantly increased following n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV.					
34132788	0	45	theme	polyunsaturated	18:32	arg1	eggs					59:62	n-3 polyunsaturated fatty acids-enriched hen eggs	14:62	n-3 polyunsaturated fatty acids-enriched hen eggs consumption	14:74	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	1	46	theme	G	304:304	arg1	level					383:387	immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level	289:387	immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level	289:387	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	2	47	dep	Control-Healthy	489:503	arg1	PUFAs/day					583:591	249 mg n-3 PUFAs/day	572:591	249 mg n-3 PUFAs/day	572:591	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	2	47	dep	Control-Healthy	489:503	arg1	eggs/daily					560:569	[consumed three regular hens' eggs/daily	530:569	[consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)]	530:593	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	4	48	theme	dietary	962:968	arg1	protocols					970:978	dietary protocols	962:978	dietary protocols	962:978	Total plasma protein and IgG N-glycome have been profiled before and after dietary protocols.					
34132788	0	49	theme	acids-enriched	40:53	arg1	eggs					59:62	n-3 polyunsaturated fatty acids-enriched hen eggs	14:62	n-3 polyunsaturated fatty acids-enriched hen eggs consumption	14:74	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	7	50	theme	bisecting	1250:1258	arg1	B					1281:1281	B	1281:1281	B	1281:1281	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.					
34132788	7	50	theme	bisecting	1250:1258	arg1	N-acetylglucosamine					1260:1278	bisecting N-acetylglucosamine	1250:1278	bisecting N-acetylglucosamine (B)	1250:1282	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.					
34132788	1	51	theme	fatty	232:236	arg1	acids					238:242	n-3 polyunsaturated fatty acids	212:242	n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption	212:284	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	1	51	theme	fatty	232:236	arg1	PUFAs					249:253	n-3 PUFAs	245:253	n-3 PUFAs	245:253	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	8	52	theme	neutral	1666:1672	arg1	S0					1683:1684	S0	1683:1684	S0	1683:1684	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	52	theme	neutral	1666:1672	arg1	glycans					1674:1680	neutral glycans	1666:1680	neutral glycans (S0)	1666:1685	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	7	53	theme	IgG	1229:1231	arg1	traits					1242:1247	Derived IgG N-glycan traits	1221:1247	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.	1221:1429	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.					
34132788	0	54	from	N-glycosylation	108:122	arg1	individuals					135:145	healthy individuals	127:145	healthy individuals	127:145	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	0	54	from	N-glycosylation	108:122	arg1	patients					166:173	cardiovascular patients	151:173	cardiovascular patients	151:173	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	10	55	theme	PUFAs	1889:1893	arg1	consumption					1895:1905	n-3 PUFAs consumption	1885:1905	n-3 PUFAs consumption	1885:1905	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	2	56	theme	n-3	579:581	arg1	PUFAs/day					583:591	249 mg n-3 PUFAs/day	572:591	249 mg n-3 PUFAs/day	572:591	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	2	56	theme	n-3	579:581	arg1	eggs/daily					560:569	[consumed three regular hens' eggs/daily	530:569	[consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)]	530:593	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	10	57	theme	inflammatory	2054:2065	arg1	profile					2083:2089	less inflammatory N-glycosylation profile	2049:2089	less inflammatory N-glycosylation profile	2049:2089	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	1	58	theme	cardiovascular	425:438	arg1	patients					445:452	cardiovascular (CV) patients	425:452	cardiovascular (CV) patients (N = 21)	425:461	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	1	58	theme	cardiovascular	425:438	arg1	CV					441:442	CV	441:442	CV	441:442	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	1	58	theme	cardiovascular	425:438	arg1	N = 21					455:460	N = 21	455:460	N = 21	455:460	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	1	59	theme	-enriched	255:263	arg1	consumption					274:284	n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption	212:284	n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption	212:284	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	10	60	theme	N-glycan	1918:1925	arg1	traits					1927:1932	IgG N-glycan traits	1914:1932	IgG N-glycan traits	1914:1932	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	8	61	theme	oligomannose	1698:1709	arg1	structures					1711:1720	oligomannose structures	1698:1720	oligomannose structures	1698:1720	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	2	62	theme	[consumed	530:538	arg1	PUFAs/day					583:591	249 mg n-3 PUFAs/day	572:591	249 mg n-3 PUFAs/day	572:591	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	2	62	theme	[consumed	530:538	arg1	eggs/daily					560:569	[consumed three regular hens' eggs/daily	530:569	[consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)]	530:593	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	0	63	from	IgG	79:81	arg1	individuals					135:145	healthy individuals	127:145	healthy individuals	127:145	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	0	63	from	IgG	79:81	arg1	patients					166:173	cardiovascular patients	151:173	cardiovascular patients	151:173	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	2	64	theme	Control-CV	509:518	arg1	subgroups					520:528	Control-CV subgroups	509:528	Control-CV subgroups	509:528	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	8	65	theme	total	1439:1443	arg1	traits					1469:1474	Derived total plasma protein N-glycan traits	1431:1474	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.	1431:1761	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	0	66	theme	cardiovascular	151:164	arg1	patients					166:173	cardiovascular patients	151:173	cardiovascular patients	151:173	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	5	67	theme	hen	1055:1057	arg1	eggs					1059:1062	n-3 PUFAs hen eggs	1045:1062	n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV	1045:1117	Serum n-3 PUFAs concentration significantly increased following n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV.					
34132788	0	68	gly	N-glycosylation	108:122	arg1	individuals					135:145	healthy individuals	127:145	healthy individuals	127:145	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	0	68	gly	N-glycosylation	108:122	arg1	patients					166:173	cardiovascular patients	151:173	cardiovascular patients	151:173	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	5	69	theme	n-3	1106:1108	arg1	PUFAs-CV					1110:1117	n-3 PUFAs-CV	1106:1117	n-3 PUFAs-CV	1106:1117	Serum n-3 PUFAs concentration significantly increased following n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV.					
34132788	8	70	theme	protein	1452:1458	arg1	traits					1469:1474	Derived total plasma protein N-glycan traits	1431:1474	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.	1431:1761	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	2	71	theme	PUFAs-enriched	665:678	arg1	eggs/daily					684:693	three n-3 PUFAs-enriched hen eggs/daily	655:693	three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks	655:730	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	2	71	theme	PUFAs-enriched	665:678	arg1	PUFAs/day					708:716	1053 mg n-3 PUFAs/day	696:716	1053 mg n-3 PUFAs/day	696:716	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	3	72	theme	Serum-free	733:742	arg1	10					823:824	10	823:824	10	823:824	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	72	theme	Serum-free	733:742	arg1	protein					796:802	high-sensitivity C-reactive protein	768:802	high-sensitivity C-reactive protein	768:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	72	theme	Serum-free	733:742	arg1	acids					750:754	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	72	theme	Serum-free	733:742	arg1	alpha					866:870	tumor necrosis factor alpha	844:870	tumor necrosis factor alpha	844:870	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	72	theme	Serum-free	733:742	arg1	interleukin					805:815	interleukin 6 and 10	805:824	interleukin	805:815	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	72	theme	Serum-free	733:742	arg1	profile					756:762	profile	756:762	profile	756:762	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	2	73	theme	regular	546:552	arg1	hens					554:557	three regular hens'	540:558	[consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)]	530:593	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	3	74	theme	C-reactive	785:794	arg1	protein					796:802	high-sensitivity C-reactive protein	768:802	high-sensitivity C-reactive protein	768:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	74	theme	C-reactive	785:794	arg1	acids					750:754	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	0	75	theme	consumption	64:74	arg1	effect					4:9	The effect	0:9	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.	0:174	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	8	76	theme	high	1477:1480	arg1	trigalactosylation					1501:1518	trigalactosylation	1501:1518	trigalactosylation	1501:1518	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	76	theme	high	1477:1480	arg1	tetragalactosylation					1521:1540	tetragalactosylation	1521:1540	tetragalactosylation	1521:1540	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	76	theme	high	1477:1480	arg1	fucosylation					1590:1601	antennary fucosylation	1580:1601	antennary fucosylation	1580:1601	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	76	theme	high	1477:1480	arg1	glycans					1492:1498	high branching glycans	1477:1498	high branching glycans	1477:1498	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	76	theme	high	1477:1480	arg1	trisialylation					1543:1556	trisialylation	1543:1556	trisialylation	1543:1556	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	76	theme	high	1477:1480	arg1	tetrasialylation					1559:1574	tetrasialylation	1559:1574	tetrasialylation	1559:1574	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	2	77	theme	1053 mg	696:702	arg1	eggs/daily					684:693	three n-3 PUFAs-enriched hen eggs/daily	655:693	three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks	655:730	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	2	77	theme	1053 mg	696:702	arg1	PUFAs/day					708:716	1053 mg n-3 PUFAs/day	696:716	1053 mg n-3 PUFAs/day	696:716	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	10	78	theme	plasma	1978:1983	arg1	traits					2002:2007	total plasma protein N-glycan traits	1972:2007	total plasma protein N-glycan traits in CV patients	1972:2022	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	1	79	theme	total	316:320	arg1	profiles					346:353	total plasma protein N-glycan profiles	316:353	total plasma protein N-glycan profiles	316:353	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	10	80	from	IL-10	1938:1942	arg1	patients					2015:2022	CV patients	2012:2022	CV patients	2012:2022	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	10	80	from	IL-10	1938:1942	arg1	individuals					1955:1965	healthy individuals	1947:1965	healthy individuals	1947:1965	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	1	81	theme	N-glycan	337:344	arg1	profiles					346:353	total plasma protein N-glycan profiles	316:353	total plasma protein N-glycan profiles	316:353	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	0	82	from	effect	4:9	arg1	N-glycosylation					108:122	total plasma protein N-glycosylation	87:122	total plasma protein N-glycosylation	87:122	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	0	82	from	effect	4:9	arg1	IgG					79:81	IgG	79:81	IgG	79:81	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	2	83	theme	n-3	622:624	arg1	subgroups					635:643	n-3 PUFAs-CV subgroups	622:643	n-3 PUFAs-CV subgroups	622:643	Subjects were divided to Control-Healthy and Control-CV subgroups [consumed three regular hens' eggs/daily (249 mg n-3 PUFAs/day)], and n-3 PUFAs-Healthy and n-3 PUFAs-CV subgroups [consumed three n-3 PUFAs-enriched hen eggs/daily (1053 mg n-3 PUFAs/day)] for 3 weeks.					
34132788	0	84	theme	total	87:91	arg1	N-glycosylation					108:122	total plasma protein N-glycosylation	87:122	total plasma protein N-glycosylation	87:122	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	1	85	theme	protein	329:335	arg1	profiles					346:353	total plasma protein N-glycan profiles	316:353	total plasma protein N-glycan profiles	316:353	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	4	86	theme	plasma	893:898	arg1	protein					900:906	Total plasma protein	887:906	Total plasma protein	887:906	Total plasma protein and IgG N-glycome have been profiled before and after dietary protocols.					
34132788	0	87	theme	protein	100:106	arg1	N-glycosylation					108:122	total plasma protein N-glycosylation	87:122	total plasma protein N-glycosylation	87:122	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	1	88	theme	profiles	346:353	arg1	level					383:387	immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level	289:387	immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level	289:387	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	3	89	dep	acids	750:754	arg1	profile					756:762	profile	756:762	profile	756:762	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	89	dep	acids	750:754	arg1	protein					796:802	high-sensitivity C-reactive protein	768:802	high-sensitivity C-reactive protein	768:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	89	dep	acids	750:754	arg1	acids					750:754	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	90	theme	necrosis	850:857	arg1	alpha					866:870	tumor necrosis factor alpha	844:870	tumor necrosis factor alpha	844:870	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	3	90	theme	necrosis	850:857	arg1	acids					750:754	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein	733:802	Serum-free fatty acids profile and high-sensitivity C-reactive protein, interleukin 6 and 10 (IL-6, IL-10) and tumor necrosis factor alpha were measured.					
34132788	1	91	theme	consumption	274:284	arg1	effect					202:207	the effect	198:207	the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level	198:387	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	0	92	theme	fatty	34:38	arg1	eggs					59:62	n-3 polyunsaturated fatty acids-enriched hen eggs	14:62	n-3 polyunsaturated fatty acids-enriched hen eggs consumption	14:74	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	1	93	theme	immunoglobulin	289:302	arg1	IgG					307:309	IgG	307:309	IgG	307:309	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	1	93	theme	immunoglobulin	289:302	arg1	G					304:304	immunoglobulin G	289:304	immunoglobulin G (IgG)	289:310	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	10	94	from	traits	2002:2007	arg1	patients					2015:2022	CV patients	2012:2022	CV patients	2012:2022	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	10	94	from	traits	2002:2007	arg1	individuals					1955:1965	healthy individuals	1947:1965	healthy individuals	1947:1965	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	8	95	theme	antennary	1580:1588	arg1	glycans					1492:1498	high branching glycans	1477:1498	high branching glycans	1477:1498	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	8	95	theme	antennary	1580:1588	arg1	fucosylation					1590:1601	antennary fucosylation	1580:1601	antennary fucosylation	1580:1601	Derived total plasma protein N-glycan traits: high branching glycans, trigalactosylation, tetragalactosylation, trisialylation, tetrasialylation and antennary fucosylation significantly decreased, whereas G0, monogalactosylation (G1), neutral glycans (S0), B, CF and oligomannose structures significantly increased in n-3 PUFAs-CV.					
34132788	0	96	theme	hen	55:57	arg1	eggs					59:62	n-3 polyunsaturated fatty acids-enriched hen eggs	14:62	n-3 polyunsaturated fatty acids-enriched hen eggs consumption	14:74	The effect of n-3 polyunsaturated fatty acids-enriched hen eggs consumption on IgG and total plasma protein N-glycosylation in healthy individuals and cardiovascular patients.					
34132788	10	97	theme	total	1972:1976	arg1	traits					2002:2007	total plasma protein N-glycan traits	1972:2007	total plasma protein N-glycan traits in CV patients	1972:2022	n-3 PUFAs consumption alters IgG N-glycan traits and IL-10 in healthy individuals, and total plasma protein N-glycan traits in CV patients, by shifting them toward less inflammatory N-glycosylation profile.					
34132788	5	98	theme	Serum	981:985	arg1	concentration					997:1009	Serum n-3 PUFAs concentration	981:1009	Serum n-3 PUFAs concentration	981:1009	Serum n-3 PUFAs concentration significantly increased following n-3 PUFAs hen eggs consumption in both n-3 PUFAs-Healthy and n-3 PUFAs-CV.					
34132788	7	99	theme	N-glycan	1233:1240	arg1	traits					1242:1247	Derived IgG N-glycan traits	1221:1247	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.	1221:1429	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.					
34132788	1	100	theme	acids	238:242	arg1	consumption					274:284	n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption	212:284	n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption	212:284	This study determined the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched hen eggs consumption on immunoglobulin G (IgG) and total plasma protein N-glycan profiles and inflammatory biomarkers level in healthy individuals (N = 33) and cardiovascular (CV) patients (N = 21).					
34132788	7	101	theme	Derived	1221:1227	arg1	traits					1242:1247	Derived IgG N-glycan traits	1221:1247	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.	1221:1429	Derived IgG N-glycan traits: bisecting N-acetylglucosamine (B) significantly decreased in n-3 PUFAs-Healthy, whereas agalactosylation (G0) and core fucosylation (CF) significantly increased in Control-Healthy.					
34019795	5	0	theme	FDG	721:723	arg1	Abs					725:727	FDG Abs	721:727	FDG Abs	721:727	FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike.					
34019795	6	1	theme	FDG	876:878	arg1	precursors					880:889	FDG precursors	876:889	FDG precursors	876:889	FDG precursors were expanded by glycan-bearing immunogens in macaques and were abundant in HIV-1-naive humans.					
34019795	3	2	theme	heavy-chain	451:461	arg1	architecture					483:494	a unique heavy-chain (VH) domain-swapped architecture	442:494	a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	442:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	6	3	theme	HIV-1-naive	967:977	arg1	humans					979:984	HIV-1-naive humans	967:984	HIV-1-naive humans	967:984	FDG precursors were expanded by glycan-bearing immunogens in macaques and were abundant in HIV-1-naive humans.					
34019795	6	4	from	immunogens	923:932	arg1	macaques					937:944	macaques	937:944	macaques	937:944	FDG precursors were expanded by glycan-bearing immunogens in macaques and were abundant in HIV-1-naive humans.					
34019795	6	5	from	humans	979:984	arg1	abundant					955:962	abundant	955:962	abundant	955:962	FDG precursors were expanded by glycan-bearing immunogens in macaques and were abundant in HIV-1-naive humans.					
34019795	4	6	theme	VH-swapped	635:644	arg1	domains					646:652	VH-swapped domains	635:652	VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques	635:718	Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques.					
34019795	3	7	theme	Fab	538:540	arg1	dimerization					543:554	fragment antigen-binding (Fab) dimerization	512:554	fragment antigen-binding (Fab) dimerization	512:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	3	8	theme	neutralizing	328:339	arg1	bnAb					345:348	bnAb	345:348	bnAb	345:348	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	3	8	theme	neutralizing	328:339	arg1	Ab					341:342	a broadly neutralizing Ab	318:342	a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	318:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	3	8	theme	neutralizing	328:339	arg1	2G12					310:313	2G12	310:313	2G12	310:313	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	4	9	theme	virus	689:693	arg1	macaques					711:718	simian-human immunodeficiency virus (SHIV)-infected macaques	659:718	simian-human immunodeficiency virus (SHIV)-infected macaques	659:718	Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques.					
34019795	4	10	theme	immunodeficiency	672:687	arg1	SHIV					696:699	SHIV	696:699	SHIV	696:699	Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques.					
34019795	4	10	theme	immunodeficiency	672:687	arg1	virus					689:693	simian-human immunodeficiency virus	659:693	simian-human immunodeficiency virus (SHIV)-infected macaques	659:718	Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques.					
34019795	2	11	gly	glycosylated	256:267	arg1	envelope					275:282	glycosylated HIV-1 envelope	256:282	glycosylated HIV-1 envelope (Env)	256:288	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	2	11	gly	glycosylated	256:267	arg1	antigen					301:307	a model antigen	293:307	a model antigen	293:307	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	2	11	gly	glycosylated	256:267	arg1	Env					285:287	Env	285:287	Env	285:287	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	5	12	theme	spike	869:873	arg1	syndrome					833:840	severe acute respiratory syndrome	808:840	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike	808:873	FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike.					
34019795	6	13	from	abundant	955:962	arg1	humans					979:984	HIV-1-naive humans	967:984	HIV-1-naive humans	967:984	FDG precursors were expanded by glycan-bearing immunogens in macaques and were abundant in HIV-1-naive humans.					
34019795	4	14	from	macaques	711:718	arg1	domains					646:652	VH-swapped domains	635:652	VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques	635:718	Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques.					
34019795	2	15	theme	HIV-1	269:273	arg1	envelope					275:282	glycosylated HIV-1 envelope	256:282	glycosylated HIV-1 envelope (Env)	256:288	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	2	15	theme	HIV-1	269:273	arg1	antigen					301:307	a model antigen	293:307	a model antigen	293:307	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	2	15	theme	HIV-1	269:273	arg1	Env					285:287	Env	285:287	Env	285:287	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	4	16	theme	-reactive	611:619	arg1	bnAbs					621:625	HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs	575:625	HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs	575:625	Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques.					
34019795	1	17	theme	pathogens	157:165	arg1	surface					146:152	the surface	142:152	the surface of pathogens	142:165	Natural antibodies (Abs) can target host glycans on the surface of pathogens.					
34019795	1	18	theme	Natural	90:96	arg1	Abs					110:112	Abs	110:112	Abs	110:112	Natural antibodies (Abs) can target host glycans on the surface of pathogens.					
34019795	1	18	theme	Natural	90:96	arg1	antibodies					98:107	Natural antibodies	90:107	Natural antibodies (Abs)	90:113	Natural antibodies (Abs) can target host glycans on the surface of pathogens.					
34019795	5	19	theme	respiratory	821:831	arg1	syndrome					833:840	severe acute respiratory syndrome	808:840	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike	808:873	FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike.					
34019795	0	20	theme	glycan-reactive	14:28	arg1	antibodies					30:39	Fab-dimerized glycan-reactive antibodies	0:39	Fab-dimerized glycan-reactive antibodies	0:39	Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.					
34019795	0	20	theme	glycan-reactive	14:28	arg1	category					58:65	a structural category	45:65	a structural category of natural antibodies	45:87	Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.					
34019795	3	21	theme	antigen-binding	521:535	arg1	dimerization					543:554	fragment antigen-binding (Fab) dimerization	512:554	fragment antigen-binding (Fab) dimerization	512:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	3	22	theme	glycan	376:381	arg1	patch					383:387	a conserved glycan patch	364:387	a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	364:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	0	23	theme	Fab-dimerized	0:12	arg1	antibodies					30:39	Fab-dimerized glycan-reactive antibodies	0:39	Fab-dimerized glycan-reactive antibodies	0:39	Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.					
34019795	0	23	theme	Fab-dimerized	0:12	arg1	category					58:65	a structural category	45:65	a structural category of natural antibodies	45:87	Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.					
34019795	5	24	theme	acute	815:819	arg1	syndrome					833:840	severe acute respiratory syndrome	808:840	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike	808:873	FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike.					
34019795	7	25	theme	zone	1143:1146	arg1	cells					1150:1154	antigen-experienced IgM+ or marginal zone B cells	1106:1154	antigen-experienced IgM+ or marginal zone B cells	1106:1154	Moreover, FDG precursors were predominately mutated IgM+IgD+CD27+, thus suggesting that they originated from a pool of antigen-experienced IgM+ or marginal zone B cells.					
34019795	5	26	theme	diverse	769:775	arg1	syndrome					833:840	severe acute respiratory syndrome	808:840	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike	808:873	FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike.					
34019795	5	26	theme	diverse	769:775	arg1	pathogens					777:785	diverse pathogens	769:785	diverse pathogens	769:785	FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike.					
34019795	5	26	theme	diverse	769:775	arg1	yeast					798:802	yeast	798:802	yeast	798:802	FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike.					
34019795	4	27	theme	-infected	701:709	arg1	macaques					711:718	simian-human immunodeficiency virus (SHIV)-infected macaques	659:718	simian-human immunodeficiency virus (SHIV)-infected macaques	659:718	Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques.					
34019795	3	28	theme	diverse	414:420	arg1	strains					428:434	geographically diverse HIV-1 strains	399:434	geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	399:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	7	29	theme	B	1148:1148	arg1	cells					1150:1154	antigen-experienced IgM+ or marginal zone B cells	1106:1154	antigen-experienced IgM+ or marginal zone B cells	1106:1154	Moreover, FDG precursors were predominately mutated IgM+IgD+CD27+, thus suggesting that they originated from a pool of antigen-experienced IgM+ or marginal zone B cells.					
34019795	3	30	theme	domain-swapped	468:481	arg1	architecture					483:494	a unique heavy-chain (VH) domain-swapped architecture	442:494	a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	442:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	3	31	theme	fragment	512:519	arg1	dimerization					543:554	fragment antigen-binding (Fab) dimerization	512:554	fragment antigen-binding (Fab) dimerization	512:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	3	32	theme	HIV-1	422:426	arg1	strains					428:434	geographically diverse HIV-1 strains	399:434	geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	399:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	0	33	theme	structural	47:56	arg1	antibodies					30:39	Fab-dimerized glycan-reactive antibodies	0:39	Fab-dimerized glycan-reactive antibodies	0:39	Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.					
34019795	0	33	theme	structural	47:56	arg1	category					58:65	a structural category	45:65	a structural category of natural antibodies	45:87	Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.					
34019795	2	34	theme	rhesus	223:228	arg1	macaques					230:237	rhesus macaques	223:237	rhesus macaques	223:237	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	7	35	theme	antigen-experienced	1106:1124	arg1	cells					1150:1154	antigen-experienced IgM+ or marginal zone B cells	1106:1154	antigen-experienced IgM+ or marginal zone B cells	1106:1154	Moreover, FDG precursors were predominately mutated IgM+IgD+CD27+, thus suggesting that they originated from a pool of antigen-experienced IgM+ or marginal zone B cells.					
34019795	3	36	theme	conserved	366:374	arg1	patch					383:387	a conserved glycan patch	364:387	a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	364:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	6	37	theme	glycan-bearing	908:921	arg1	immunogens					923:932	glycan-bearing immunogens	908:932	glycan-bearing immunogens in macaques	908:944	FDG precursors were expanded by glycan-bearing immunogens in macaques and were abundant in HIV-1-naive humans.					
34019795	7	38	theme	marginal	1134:1141	arg1	cells					1150:1154	antigen-experienced IgM+ or marginal zone B cells	1106:1154	antigen-experienced IgM+ or marginal zone B cells	1106:1154	Moreover, FDG precursors were predominately mutated IgM+IgD+CD27+, thus suggesting that they originated from a pool of antigen-experienced IgM+ or marginal zone B cells.					
34019795	5	39	theme	severe	808:813	arg1	syndrome					833:840	severe acute respiratory syndrome	808:840	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike	808:873	FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike.					
34019795	5	40	theme	cell-surface	745:756	arg1	glycans					758:764	cell-surface glycans	745:764	cell-surface glycans	745:764	FDG Abs also recognized cell-surface glycans on diverse pathogens, including yeast and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike.					
34019795	2	41	theme	cells	214:218	arg1	evolution					183:191	the evolution	179:191	the evolution of glycan-reactive B cells of rhesus macaques and humans	179:248	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	4	42	theme	simian-human	659:670	arg1	SHIV					696:699	SHIV	696:699	SHIV	696:699	Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques.					
34019795	4	42	theme	simian-human	659:670	arg1	virus					689:693	simian-human immunodeficiency virus	659:693	simian-human immunodeficiency virus (SHIV)-infected macaques	659:718	Here, we describe HIV-1 Env Fab-dimerized glycan (FDG)-reactive bnAbs without VH-swapped domains from simian-human immunodeficiency virus (SHIV)-infected macaques.					
34019795	7	43	theme	IgM+	1126:1129	arg1	cells					1150:1154	antigen-experienced IgM+ or marginal zone B cells	1106:1154	antigen-experienced IgM+ or marginal zone B cells	1106:1154	Moreover, FDG precursors were predominately mutated IgM+IgD+CD27+, thus suggesting that they originated from a pool of antigen-experienced IgM+ or marginal zone B cells.					
34019795	2	44	theme	glycan-reactive	196:210	arg1	cells					214:218	glycan-reactive B cells	196:218	glycan-reactive B cells of rhesus macaques and humans	196:248	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	2	45	theme	B	212:212	arg1	cells					214:218	glycan-reactive B cells	196:218	glycan-reactive B cells of rhesus macaques and humans	196:248	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	3	46	theme	unique	444:449	arg1	architecture					483:494	a unique heavy-chain (VH) domain-swapped architecture	442:494	a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	442:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	7	47	theme	FDG	997:999	arg1	precursors					1001:1010	FDG precursors	997:1010	FDG precursors	997:1010	Moreover, FDG precursors were predominately mutated IgM+IgD+CD27+, thus suggesting that they originated from a pool of antigen-experienced IgM+ or marginal zone B cells.					
34019795	1	48	theme	host	126:129	arg1	glycans					131:137	host glycans	126:137	host glycans	126:137	Natural antibodies (Abs) can target host glycans on the surface of pathogens.					
34019795	2	49	theme	glycosylated	256:267	arg1	envelope					275:282	glycosylated HIV-1 envelope	256:282	glycosylated HIV-1 envelope (Env)	256:288	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	2	49	theme	glycosylated	256:267	arg1	antigen					301:307	a model antigen	293:307	a model antigen	293:307	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	2	49	theme	glycosylated	256:267	arg1	Env					285:287	Env	285:287	Env	285:287	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	2	50	theme	humans	243:248	arg1	cells					214:218	glycan-reactive B cells	196:218	glycan-reactive B cells of rhesus macaques and humans	196:248	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	2	51	theme	model	295:299	arg1	envelope					275:282	glycosylated HIV-1 envelope	256:282	glycosylated HIV-1 envelope (Env)	256:288	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	2	51	theme	model	295:299	arg1	antigen					301:307	a model antigen	293:307	a model antigen	293:307	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	3	52	theme	strains	428:434	arg1	Env					392:394	Env	392:394	Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	392:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	0	53	theme	antibodies	78:87	arg1	antibodies					30:39	Fab-dimerized glycan-reactive antibodies	0:39	Fab-dimerized glycan-reactive antibodies	0:39	Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.					
34019795	0	53	theme	antibodies	78:87	arg1	category					58:65	a structural category	45:65	a structural category of natural antibodies	45:87	Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.					
34019795	3	54	from	patch	383:387	arg1	Env					392:394	Env	392:394	Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	392:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	3	55	theme	VH	464:465	arg1	architecture					483:494	a unique heavy-chain (VH) domain-swapped architecture	442:494	a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization	442:554	2G12 is a broadly neutralizing Ab (bnAb) that targets a conserved glycan patch on Env of geographically diverse HIV-1 strains using a unique heavy-chain (VH) domain-swapped architecture that results in fragment antigen-binding (Fab) dimerization.					
34019795	0	56	theme	natural	70:76	arg1	antibodies					78:87	natural antibodies	70:87	natural antibodies	70:87	Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies.					
34019795	2	57	theme	macaques	230:237	arg1	cells					214:218	glycan-reactive B cells	196:218	glycan-reactive B cells of rhesus macaques and humans	196:248	We studied the evolution of glycan-reactive B cells of rhesus macaques and humans using glycosylated HIV-1 envelope (Env) as a model antigen.					
34019795	7	58	theme	cells	1150:1154	arg1	pool					1098:1101	a pool	1096:1101	a pool of antigen-experienced IgM+ or marginal zone B cells	1096:1154	Moreover, FDG precursors were predominately mutated IgM+IgD+CD27+, thus suggesting that they originated from a pool of antigen-experienced IgM+ or marginal zone B cells.					
32483376	0	0	from	editing	19:25	arg1	surfaces					42:49	living cell surfaces	30:49	living cell surfaces	30:49	Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.					
32483376	4	1	theme	N-glycan-subtype-selective	733:758	arg1	remodeling					760:769	N-glycan-subtype-selective remodeling	733:769	N-glycan-subtype-selective remodeling	733:769	In particular, N-glycan-subtype-selective remodeling and imaging with different monosaccharide motifs at the non-reducing end were successfully achieved.					
32483376	2	2	from	editing	330:336	arg1	surface					345:351	the surface	341:351	the surface of living cells	341:367	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	2	3	theme	insert	481:486	arg1	step					489:492	a second 'insert' step	471:492	a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites	471:565	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	2	4	gly	N-glycoforms	432:443	arg1	subclass					458:465	a certain subclass	448:465	a certain subclass	448:465	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	5	5	theme	system	910:915	arg1	combination					880:890	a combination	878:890	a combination of the expression system of the Lec4 CHO cell line	878:941	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	5	theme	system	910:915	arg1	delta					1002:1006	opioid receptor delta 1	986:1008	opioid receptor delta 1 (OPRD1)	986:1016	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	5	theme	system	910:915	arg1	approach					976:983	this two-step glycan-editing approach	947:983	this two-step glycan-editing approach	947:983	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	4	6	with	imaging	775:781	arg1	motifs					813:818	different monosaccharide motifs	788:818	different monosaccharide motifs at the non-reducing end	788:842	In particular, N-glycan-subtype-selective remodeling and imaging with different monosaccharide motifs at the non-reducing end were successfully achieved.					
32483376	4	7	theme	monosaccharide	798:811	arg1	motifs					813:818	different monosaccharide motifs	788:818	different monosaccharide motifs at the non-reducing end	788:842	In particular, N-glycan-subtype-selective remodeling and imaging with different monosaccharide motifs at the non-reducing end were successfully achieved.					
32483376	4	8	with	remodeling	760:769	arg1	motifs					813:818	different monosaccharide motifs	788:818	different monosaccharide motifs at the non-reducing end	788:842	In particular, N-glycan-subtype-selective remodeling and imaging with different monosaccharide motifs at the non-reducing end were successfully achieved.					
32483376	4	9	from	end	840:842	arg1	motifs					813:818	different monosaccharide motifs	788:818	different monosaccharide motifs at the non-reducing end	788:842	In particular, N-glycan-subtype-selective remodeling and imaging with different monosaccharide motifs at the non-reducing end were successfully achieved.					
32483376	2	10	theme	N-glycan-subtype-selective	303:328	arg1	editing					330:336	N-glycan-subtype-selective editing	303:336	N-glycan-subtype-selective editing on the surface of living cells	303:367	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	2	11	theme	well-defined	508:519	arg1	N-glycan					521:528	a well-defined N-glycan	506:528	a well-defined N-glycan	506:528	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	5	12	theme	two-step	952:959	arg1	delta					1002:1006	opioid receptor delta 1	986:1008	opioid receptor delta 1 (OPRD1)	986:1016	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	12	theme	two-step	952:959	arg1	approach					976:983	this two-step glycan-editing approach	947:983	this two-step glycan-editing approach	947:983	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	3	13	contain	carrying	593:600	arg1	cells					586:590	Such glyco-edited cells	568:590	Such glyco-edited cells	568:590	Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions.					
32483376	3	13	contain	carrying	593:600	arg2	structures					635:644	more homogeneous oligosaccharide structures	602:644	more homogeneous oligosaccharide structures	602:644	Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions.					
32483376	1	14	gly	glycosylated	103:114	arg1	ways					127:130	various ways	119:130	various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions	119:243	Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions.					
32483376	1	14	gly	glycosylated	103:114	arg1	surfaces					90:97	Cell surfaces	85:97	Cell surfaces	85:97	Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions.					
32483376	1	14	gly	glycosylated	103:114	arg2	surfaces					90:97	Cell surfaces	85:97	Cell surfaces	85:97	Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions.					
32483376	1	15	with	ways	127:130	arg1	heterogeneity					142:154	high heterogeneity	137:154	high heterogeneity	137:154	Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions.					
32483376	2	16	theme	heterogeneous	418:430	arg1	N-glycoforms					432:443	heterogeneous N-glycoforms	418:443	heterogeneous N-glycoforms of a certain subclass	418:465	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	5	17	theme	cell	933:936	arg1	line					938:941	the Lec4 CHO cell line	920:941	the Lec4 CHO cell line	920:941	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	18	theme	Lec4	924:927	arg1	line					938:941	the Lec4 CHO cell line	920:941	the Lec4 CHO cell line	920:941	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	2	19	theme	chemoenzymatic	275:288	arg1	approach					290:297	a two-step chemoenzymatic approach	264:297	a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites	264:565	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	5	20	theme	CHO	929:931	arg1	line					938:941	the Lec4 CHO cell line	920:941	the Lec4 CHO cell line	920:941	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	0	21	theme	N-glycan	10:17	arg1	editing					19:25	Selective N-glycan editing	0:25	Selective N-glycan editing on living cell surfaces	0:49	Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.					
32483376	1	22	theme	Cell	85:88	arg1	surfaces					90:97	Cell surfaces	85:97	Cell surfaces	85:97	Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions.					
32483376	2	23	theme	delete	395:400	arg1	step					403:406	a first 'delete' step	386:406	a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass	386:465	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	0	24	theme	Selective	0:8	arg1	editing					19:25	Selective N-glycan editing	0:25	Selective N-glycan editing on living cell surfaces	0:49	Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.					
32483376	5	25	theme	agonist-induced	1124:1138	arg1	functions					1088:1096	the biological functions	1073:1096	the biological functions of receptor dimerization	1073:1121	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	25	theme	agonist-induced	1124:1138	arg1	signaling					1140:1148	agonist-induced signaling	1124:1148	agonist-induced signaling	1124:1148	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	3	26	theme	glyco-edited	573:584	arg1	cells					586:590	Such glyco-edited cells	568:590	Such glyco-edited cells	568:590	Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions.					
32483376	1	27	theme	ambiguous	180:188	arg1	conclusions					190:200	ambiguous conclusions	180:200	ambiguous conclusions about glycan-involved biological functions	180:243	Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions.					
32483376	3	28	theme	homogeneous	607:617	arg1	structures					635:644	more homogeneous oligosaccharide structures	602:644	more homogeneous oligosaccharide structures	602:644	Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions.					
32483376	2	29	theme	pretreated	544:553	arg1	glyco-sites					555:565	the pretreated glyco-sites	540:565	the pretreated glyco-sites	540:565	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	5	30	theme	receptor	1101:1108	arg1	dimerization					1110:1121	receptor dimerization	1101:1121	receptor dimerization	1101:1121	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	31	theme	expression	899:908	arg1	system					910:915	the expression system	895:915	the expression system of the Lec4 CHO cell line	895:941	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	4	32	theme	different	788:796	arg1	motifs					813:818	different monosaccharide motifs	788:818	different monosaccharide motifs at the non-reducing end	788:842	In particular, N-glycan-subtype-selective remodeling and imaging with different monosaccharide motifs at the non-reducing end were successfully achieved.					
32483376	2	33	theme	certain	450:456	arg1	subclass					458:465	a certain subclass	448:465	a certain subclass	448:465	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	3	34	theme	precise	660:666	arg1	understanding					668:680	precise understanding	660:680	precise understanding of carbohydrate-mediated functions	660:715	Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions.					
32483376	5	35	theme	glycan-editing	961:974	arg1	delta					1002:1006	opioid receptor delta 1	986:1008	opioid receptor delta 1 (OPRD1)	986:1016	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	35	theme	glycan-editing	961:974	arg1	approach					976:983	this two-step glycan-editing approach	947:983	this two-step glycan-editing approach	947:983	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	2	36	theme	first	388:392	arg1	step					403:406	a first 'delete' step	386:406	a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass	386:465	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	0	37	theme	cell	37:40	arg1	surfaces					42:49	living cell surfaces	30:49	living cell surfaces	30:49	Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.					
32483376	2	38	theme	cells	363:367	arg1	surface					345:351	the surface	341:351	the surface of living cells	341:367	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	5	39	theme	line	938:941	arg1	system					910:915	the expression system	895:915	the expression system of the Lec4 CHO cell line	895:941	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	1	40	theme	glycan-involved	208:222	arg1	functions					235:243	glycan-involved biological functions	208:243	glycan-involved biological functions	208:243	Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions.					
32483376	0	41	theme	living	30:35	arg1	surfaces					42:49	living cell surfaces	30:49	living cell surfaces	30:49	Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.					
32483376	2	42	theme	second	473:478	arg1	step					489:492	a second 'insert' step	471:492	a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites	471:565	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	5	43	theme	biological	1077:1086	arg1	internalization					1154:1168	internalization	1154:1168	internalization	1154:1168	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	43	theme	biological	1077:1086	arg1	functions					1088:1096	the biological functions	1073:1096	the biological functions of receptor dimerization	1073:1121	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	43	theme	biological	1077:1086	arg1	signaling					1140:1148	agonist-induced signaling	1124:1148	agonist-induced signaling	1124:1148	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	1	44	theme	biological	224:233	arg1	functions					235:243	glycan-involved biological functions	208:243	glycan-involved biological functions	208:243	Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions.					
32483376	3	45	theme	functions	707:715	arg1	understanding					668:680	precise understanding	660:680	precise understanding of carbohydrate-mediated functions	660:715	Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions.					
32483376	1	46	theme	various	119:125	arg1	ways					127:130	various ways	119:130	various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions	119:243	Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions.					
32483376	3	47	theme	Such	568:571	arg1	cells					586:590	Such glyco-edited cells	568:590	Such glyco-edited cells	568:590	Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions.					
32483376	5	48	theme	dimerization	1110:1121	arg1	internalization					1154:1168	internalization	1154:1168	internalization	1154:1168	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	48	theme	dimerization	1110:1121	arg1	functions					1088:1096	the biological functions	1073:1096	the biological functions of receptor dimerization	1073:1121	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	48	theme	dimerization	1110:1121	arg1	signaling					1140:1148	agonist-induced signaling	1124:1148	agonist-induced signaling	1124:1148	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	2	49	theme	two-step	266:273	arg1	approach					290:297	a two-step chemoenzymatic approach	264:297	a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites	264:565	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	2	50	theme	living	356:361	arg1	cells					363:367	living cells	356:367	living cells	356:367	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	2	51	dep	step	489:492	arg1	assemble					497:504	assemble	497:504	to assemble a well-defined N-glycan back onto the pretreated glyco-sites	494:565	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	4	52	theme	non-reducing	827:838	arg1	end					840:842	the non-reducing end	823:842	the non-reducing end	823:842	In particular, N-glycan-subtype-selective remodeling and imaging with different monosaccharide motifs at the non-reducing end were successfully achieved.					
32483376	5	53	theme	receptor	993:1000	arg1	delta					1002:1006	opioid receptor delta 1	986:1008	opioid receptor delta 1 (OPRD1)	986:1016	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	53	theme	receptor	993:1000	arg1	OPRD1					1011:1015	OPRD1	1011:1015	OPRD1	1011:1015	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	53	theme	receptor	993:1000	arg1	approach					976:983	this two-step glycan-editing approach	947:983	this two-step glycan-editing approach	947:983	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	54	theme	opioid	986:991	arg1	delta					1002:1006	opioid receptor delta 1	986:1008	opioid receptor delta 1 (OPRD1)	986:1016	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	54	theme	opioid	986:991	arg1	OPRD1					1011:1015	OPRD1	1011:1015	OPRD1	1011:1015	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	5	54	theme	opioid	986:991	arg1	approach					976:983	this two-step glycan-editing approach	947:983	this two-step glycan-editing approach	947:983	Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.					
32483376	0	55	theme	glycoconjugate	60:73	arg1	function					75:82	glycoconjugate function	60:82	glycoconjugate function	60:82	Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.					
32483376	3	56	theme	carbohydrate-mediated	685:705	arg1	functions					707:715	carbohydrate-mediated functions	685:715	carbohydrate-mediated functions	685:715	Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions.					
32483376	2	57	dep	step	403:406	arg1	remove					411:416	remove	411:416	to remove heterogeneous N-glycoforms of a certain subclass	408:465	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32483376	1	58	theme	high	137:140	arg1	heterogeneity					142:154	high heterogeneity	137:154	high heterogeneity	137:154	Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions.					
32483376	3	59	theme	oligosaccharide	619:633	arg1	structures					635:644	more homogeneous oligosaccharide structures	602:644	more homogeneous oligosaccharide structures	602:644	Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions.					
32483376	2	60	theme	subclass	458:465	arg1	N-glycoforms					432:443	heterogeneous N-glycoforms	418:443	heterogeneous N-glycoforms of a certain subclass	418:465	Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites.					
32163462	2	0	attach	present	362:368	arg2	we					359:360	we	359:360	we	359:360	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	2	0	attach	present	362:368	arg1	study					352:356	this study	347:356	this study	347:356	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	1	1	theme	special	164:170	arg1	challenge					172:180	a special challenge	162:180	a special challenge	162:180	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	5	2	theme	lower	921:925	arg1	degree					927:932	a lower degree	919:932	a lower degree of sialylation	919:947	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	0	3	theme	transfection	83:94	arg1	function-Effect					44:58	biological function-Effect	33:58	biological function-Effect of expression host and transfection on IgM properties	33:112	Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties.					
32163462	3	4	theme	classical	689:697	arg1	pathway					710:716	the classical complement pathway	685:716	the classical complement pathway	685:716	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	4	5	theme	polymer	802:808	arg1	distribution					810:821	polymer distribution	802:821	polymer distribution	802:821	The HEK293E transient expression system is able to generate comparable amounts and polymer distribution as IgM stably produced in CHO.					
32163462	1	6	theme	elucidated	251:260	arg1	interactions					262:273	their not yet fully elucidated interactions	231:273	their not yet fully elucidated interactions with the immune effector proteins	231:307	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	6	7	theme	IgM	1124:1126	arg1	pentamers					1128:1136	IgM pentamers	1124:1136	IgM pentamers produced in HEK293E cells	1124:1162	Electron microscopy also confirmed the structural integrity of IgM pentamers produced in HEK293E cells, since the conventional star-shaped structure is observed.					
32163462	3	8	theme	complement	699:708	arg1	pathway					710:716	the classical complement pathway	685:716	the classical complement pathway	685:716	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	7	9	theme	altered	1417:1423	arg1	structure					1474:1482	comparable structure	1463:1482	comparable structure	1463:1482	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	7	9	theme	altered	1417:1423	arg1	modifications					1444:1456	slightly altered post-translational modifications	1408:1456	slightly altered post-translational modifications	1408:1456	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	7	9	theme	altered	1417:1423	arg1	function					1488:1495	function	1488:1495	function	1488:1495	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	4	10	theme	comparable	779:788	arg1	amounts					790:796	comparable amounts	779:796	comparable amounts	779:796	The HEK293E transient expression system is able to generate comparable amounts and polymer distribution as IgM stably produced in CHO.					
32163462	3	11	theme	presented	536:544	arg1	workflow					546:553	The presented workflow	532:553	The presented workflow	532:553	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	0	12	theme	IgM	99:101	arg1	properties					103:112	IgM properties	99:112	IgM properties	99:112	Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties.					
32163462	7	13	theme	expression	1272:1281	arg1	system					1283:1288	the transient expression system	1258:1288	the transient expression system	1258:1288	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	5	14	theme	structures	986:995	arg1	portion					962:968	a higher portion	953:968	a higher portion of oligomannose structures	953:995	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	5	14	theme	structures	986:995	arg1	degree					927:932	a lower degree	919:932	a lower degree of sialylation	919:947	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	3	15	gly	glycosylation	633:645	arg1	pathway					710:716	the classical complement pathway	685:716	the classical complement pathway	685:716	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	2	16	theme	stable	493:498	arg1	system					511:516	the well-established stable expression system	472:516	the well-established stable expression system in CHO cells	472:529	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	5	17	theme	sialylation	937:947	arg1	portion					962:968	a higher portion	953:968	a higher portion of oligomannose structures	953:995	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	5	17	theme	sialylation	937:947	arg1	degree					927:932	a lower degree	919:932	a lower degree of sialylation	919:947	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	2	18	theme	IgM	394:396	arg1	IgM012_GL					429:437	IgM012_GL	429:437	IgM012_GL	429:437	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	2	18	theme	IgM	394:396	arg1	IgM012					418:423	IgM012	418:423	IgM012	418:423	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	2	18	theme	IgM	394:396	arg1	IgM617					410:415	IgM617	410:415	IgM617	410:415	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	2	18	theme	IgM	394:396	arg1	antibodies					398:407	IgM antibodies	394:407	IgM antibodies (IgM617, IgM012 and IgM012_GL)	394:438	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	2	19	theme	well-established	476:491	arg1	system					511:516	the well-established stable expression system	472:516	the well-established stable expression system in CHO cells	472:529	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	0	20	from	function-Effect	44:58	arg1	properties					103:112	IgM properties	99:112	IgM properties	99:112	Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties.					
32163462	6	21	theme	HEK293E	1150:1156	arg1	cells					1158:1162	HEK293E cells	1150:1162	HEK293E cells	1150:1162	Electron microscopy also confirmed the structural integrity of IgM pentamers produced in HEK293E cells, since the conventional star-shaped structure is observed.					
32163462	6	22	theme	structural	1100:1109	arg1	integrity					1111:1119	the structural integrity	1096:1119	the structural integrity of IgM pentamers produced in HEK293E cells	1096:1162	Electron microscopy also confirmed the structural integrity of IgM pentamers produced in HEK293E cells, since the conventional star-shaped structure is observed.					
32163462	0	23	theme	pentameric	10:19	arg1	IgM					21:23	Transient pentameric IgM	0:23	Transient pentameric IgM	0:23	Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties.					
32163462	5	24	theme	glycan	867:872	arg1	profile					874:880	the glycan profile	863:880	the glycan profile generated by HEK293E cells	863:907	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	6	25	theme	star-shaped	1188:1198	arg1	structure					1200:1208	the conventional star-shaped structure	1171:1208	the conventional star-shaped structure	1171:1208	Electron microscopy also confirmed the structural integrity of IgM pentamers produced in HEK293E cells, since the conventional star-shaped structure is observed.					
32163462	7	26	theme	attractive	1302:1311	arg1	alternative					1313:1323	an attractive alternative	1299:1323	an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function	1299:1495	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	7	27	theme	high-throughput	1350:1364	arg1	production					1366:1375	rapid, efficient and high-throughput production	1329:1375	rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function	1329:1495	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	0	28	theme	Transient	0:8	arg1	IgM					21:23	Transient pentameric IgM	0:23	Transient pentameric IgM	0:23	Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties.					
32163462	4	29	theme	transient	731:739	arg1	able					762:765	able	762:765	able	762:765	The HEK293E transient expression system is able to generate comparable amounts and polymer distribution as IgM stably produced in CHO.					
32163462	4	29	theme	transient	731:739	arg1	system					752:757	The HEK293E transient expression system	719:757	The HEK293E transient expression system	719:757	The HEK293E transient expression system is able to generate comparable amounts and polymer distribution as IgM stably produced in CHO.					
32163462	5	30	theme	higher	955:960	arg1	portion					962:968	a higher portion	953:968	a higher portion of oligomannose structures	953:995	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	1	31	theme	complex	193:199	arg1	structure					201:209	the complex structure	189:209	the complex structure of the proteins	189:225	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	2	32	theme	CHO	521:523	arg1	cells					525:529	CHO cells	521:529	CHO cells	521:529	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	1	33	theme	immune	284:289	arg1	proteins					300:307	the immune effector proteins	280:307	the immune effector proteins	280:307	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	7	34	theme	antibodies	1392:1401	arg1	production					1366:1375	rapid, efficient and high-throughput production	1329:1375	rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function	1329:1495	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	5	35	theme	oligomannose	973:984	arg1	structures					986:995	oligomannose structures	973:995	oligomannose structures	973:995	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	6	36	theme	conventional	1175:1186	arg1	structure					1200:1208	the conventional star-shaped structure	1171:1208	the conventional star-shaped structure	1171:1208	Electron microscopy also confirmed the structural integrity of IgM pentamers produced in HEK293E cells, since the conventional star-shaped structure is observed.					
32163462	1	37	theme	effector	291:298	arg1	proteins					300:307	the immune effector proteins	280:307	the immune effector proteins	280:307	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	3	38	theme	antibody	648:655	arg1	structure					657:665	antibody structure	648:665	antibody structure	648:665	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	1	39	theme	Recombinant	115:125	arg1	production					127:136	Recombinant production	115:136	Recombinant production of IgM antibodies	115:154	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	2	40	theme	HEK	443:445	arg1	cells					447:451	HEK cells	443:451	HEK cells	443:451	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	1	41	with	structure	201:209	arg1	proteins					300:307	the immune effector proteins	280:307	the immune effector proteins	280:307	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	7	42	theme	rapid	1329:1333	arg1	production					1366:1375	rapid, efficient and high-throughput production	1329:1375	rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function	1329:1495	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	5	43	theme	complement	1026:1035	arg1	cascade					1037:1043	the complement cascade	1022:1043	the complement cascade	1022:1043	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	2	44	theme	transient	370:378	arg1	expression					380:389	transient expression	370:389	transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells	370:451	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	6	45	theme	Electron	1061:1068	arg1	microscopy					1070:1079	Electron microscopy	1061:1079	Electron microscopy	1061:1079	Electron microscopy also confirmed the structural integrity of IgM pentamers produced in HEK293E cells, since the conventional star-shaped structure is observed.					
32163462	0	46	theme	biological	33:42	arg1	function-Effect					44:58	biological function-Effect	33:58	biological function-Effect of expression host and transfection on IgM properties	33:112	Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties.					
32163462	2	47	dep	antibodies	398:407	arg1	IgM012_GL					429:437	IgM012_GL	429:437	IgM012_GL	429:437	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	2	47	dep	antibodies	398:407	arg1	IgM012					418:423	IgM012	418:423	IgM012	418:423	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	2	47	dep	antibodies	398:407	arg1	IgM617					410:415	IgM617	410:415	IgM617	410:415	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	2	47	dep	antibodies	398:407	arg1	antibodies					398:407	IgM antibodies	394:407	IgM antibodies (IgM617, IgM012 and IgM012_GL)	394:438	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	1	48	with	interactions	262:273	arg1	proteins					300:307	the immune effector proteins	280:307	the immune effector proteins	280:307	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	1	49	theme	proteins	218:225	arg1	interactions					262:273	their not yet fully elucidated interactions	231:273	their not yet fully elucidated interactions with the immune effector proteins	231:307	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	1	49	theme	proteins	218:225	arg1	structure					201:209	the complex structure	189:209	the complex structure of the proteins	189:225	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	0	50	theme	expression	63:72	arg1	host					74:77	expression host	63:77	expression host	63:77	Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties.					
32163462	7	51	theme	IgM	1388:1390	arg1	antibodies					1392:1401	complex IgM antibodies	1380:1401	complex IgM antibodies	1380:1401	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	3	52	theme	quality	568:574	arg1	attributes					576:585	quality attributes	568:585	quality attributes	568:585	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	4	53	theme	HEK293E	723:729	arg1	able					762:765	able	762:765	able	762:765	The HEK293E transient expression system is able to generate comparable amounts and polymer distribution as IgM stably produced in CHO.					
32163462	4	53	theme	HEK293E	723:729	arg1	system					752:757	The HEK293E transient expression system	719:757	The HEK293E transient expression system	719:757	The HEK293E transient expression system is able to generate comparable amounts and polymer distribution as IgM stably produced in CHO.					
32163462	7	54	with	production	1366:1375	arg1	structure					1474:1482	comparable structure	1463:1482	comparable structure	1463:1482	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	7	54	with	production	1366:1375	arg1	modifications					1444:1456	slightly altered post-translational modifications	1408:1456	slightly altered post-translational modifications	1408:1456	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	7	54	with	production	1366:1375	arg1	function					1488:1495	function	1488:1495	function	1488:1495	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	7	55	theme	post-translational	1425:1442	arg1	structure					1474:1482	comparable structure	1463:1482	comparable structure	1463:1482	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	7	55	theme	post-translational	1425:1442	arg1	modifications					1444:1456	slightly altered post-translational modifications	1408:1456	slightly altered post-translational modifications	1408:1456	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	7	55	theme	post-translational	1425:1442	arg1	function					1488:1495	function	1488:1495	function	1488:1495	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	1	56	theme	complement	325:334	arg1	system					336:341	the complement system	321:341	the complement system	321:341	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	1	57	theme	IgM	141:143	arg1	antibodies					145:154	IgM antibodies	141:154	IgM antibodies	141:154	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	5	58	theme	HEK293E	895:901	arg1	cells					903:907	HEK293E cells	895:907	HEK293E cells	895:907	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	7	59	theme	comparable	1463:1472	arg1	structure					1474:1482	comparable structure	1463:1482	comparable structure	1463:1482	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	7	59	theme	comparable	1463:1472	arg1	modifications					1444:1456	slightly altered post-translational modifications	1408:1456	slightly altered post-translational modifications	1408:1456	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	2	60	from	system	511:516	arg1	cells					525:529	CHO cells	521:529	CHO cells	521:529	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	2	61	from	expression	380:389	arg1	cells					447:451	HEK cells	443:451	HEK cells	443:451	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	1	62	theme	antibodies	145:154	arg1	production					127:136	Recombinant production	115:136	Recombinant production of IgM antibodies	115:154	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	4	63	theme	expression	741:750	arg1	able					762:765	able	762:765	able	762:765	The HEK293E transient expression system is able to generate comparable amounts and polymer distribution as IgM stably produced in CHO.					
32163462	4	63	theme	expression	741:750	arg1	system					752:757	The HEK293E transient expression system	719:757	The HEK293E transient expression system	719:757	The HEK293E transient expression system is able to generate comparable amounts and polymer distribution as IgM stably produced in CHO.					
32163462	2	64	theme	antibodies	398:407	arg1	expression					380:389	transient expression	370:389	transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells	370:451	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
32163462	1	65	dep	structure	201:209	arg1	system					336:341	the complement system	321:341	the complement system	321:341	Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system.					
32163462	7	66	theme	efficient	1336:1344	arg1	production					1366:1375	rapid, efficient and high-throughput production	1329:1375	rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function	1329:1495	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	7	67	theme	complex	1380:1386	arg1	antibodies					1392:1401	complex IgM antibodies	1380:1401	complex IgM antibodies	1380:1401	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	0	68	theme	host	74:77	arg1	function-Effect					44:58	biological function-Effect	33:58	biological function-Effect of expression host and transfection on IgM properties	33:112	Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties.					
32163462	3	69	theme	pathway	710:716	arg1	activation					671:680	activation	671:680	activation of the classical complement pathway	671:716	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	3	69	theme	pathway	710:716	arg1	glycosylation					633:645	glycosylation	633:645	glycosylation	633:645	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	3	69	theme	pathway	710:716	arg1	productivity					597:608	productivity	597:608	productivity	597:608	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	3	69	theme	pathway	710:716	arg1	distribution					619:630	polymer distribution	611:630	polymer distribution	611:630	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	3	69	theme	pathway	710:716	arg1	structure					657:665	antibody structure	648:665	antibody structure	648:665	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	3	70	theme	polymer	611:617	arg1	distribution					619:630	polymer distribution	611:630	polymer distribution	611:630	The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway.					
32163462	5	71	contain	contained	909:917	arg2	degree					927:932	a lower degree	919:932	a lower degree of sialylation	919:947	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	5	71	contain	contained	909:917	arg1	profile					874:880	the glycan profile	863:880	the glycan profile generated by HEK293E cells	863:907	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	5	71	contain	contained	909:917	arg2	portion					962:968	a higher portion	953:968	a higher portion of oligomannose structures	953:995	Although the glycan profile generated by HEK293E cells contained a lower degree of sialylation and a higher portion of oligomannose structures, the potency to activate the complement cascade was maintained.					
32163462	6	72	theme	pentamers	1128:1136	arg1	integrity					1111:1119	the structural integrity	1096:1119	the structural integrity of IgM pentamers produced in HEK293E cells	1096:1162	Electron microscopy also confirmed the structural integrity of IgM pentamers produced in HEK293E cells, since the conventional star-shaped structure is observed.					
32163462	7	73	theme	transient	1262:1270	arg1	system					1283:1288	the transient expression system	1258:1288	the transient expression system	1258:1288	From our studies, we conclude that the transient expression system provides an attractive alternative for rapid, efficient and high-throughput production of complex IgM antibodies with slightly altered post-translational modifications, but comparable structure and function.					
32163462	2	74	theme	expression	500:509	arg1	system					511:516	the well-established stable expression system	472:516	the well-established stable expression system in CHO cells	472:529	In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells.					
33581409	3	0	from	N-glycome	689:697	arg1	cohort					748:753	a cohort	746:753	a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections	746:835	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	11	1	with	glycans	2048:2054	arg1	GlcNAc					2071:2076	bisecting GlcNAc	2061:2076	bisecting GlcNAc	2061:2076	The lectins confirmed the expression of the epitopes in cancer cells and revealed different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc.					
33581409	8	2	theme	less	1571:1574	arg1	abundance					1576:1584	less abundance	1571:1584	less abundance	1571:1584	These N-glycans were detected in less abundance in sTRA-expressing tumor tissues, which favored triantennary and tetra-antennary structures with polylactosamine extensions.					
33581409	1	3	theme	overall	247:253	arg1	likelihood					255:264	the overall likelihood	243:264	the overall likelihood of patient survival	243:284	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	3	4	theme	cancer	736:741	arg1	N-glycome					689:697	the N-glycome	685:697	the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections	685:835	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	4	5	theme	same	858:861	arg1	tissues					863:869	these same tissues	852:869	these same tissues	852:869	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	9	6	located	detected	1750:1757	arg2	sialylation					1721:1731	Increased sialylation	1711:1731	Increased sialylation of N-glycans	1711:1744	Increased sialylation of N-glycans was detected in all tumor tissues.					
33581409	9	6	located	detected	1750:1757	arg1	tissues					1772:1778	all tumor tissues	1762:1778	all tumor tissues	1762:1778	Increased sialylation of N-glycans was detected in all tumor tissues.					
33581409	5	7	theme	biomarker-categorized	1228:1248	arg1	samples					1257:1263	different biomarker-categorized tissue samples	1218:1263	different biomarker-categorized tissue samples	1218:1263	These analyses revealed distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples.					
33581409	7	8	theme	bisecting	1520:1528	arg1	GlcNAc					1530:1535	bisecting GlcNAc	1520:1535	bisecting GlcNAc	1520:1535	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	11	9	theme	epitopes	1957:1964	arg1	expression					1939:1948	the expression	1935:1948	the expression of the epitopes in cancer cells	1935:1980	The lectins confirmed the expression of the epitopes in cancer cells and revealed different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc.					
33581409	6	10	theme	normal	1334:1339	arg1	islets					1352:1357	normal pancreatic islets	1334:1357	normal pancreatic islets	1334:1357	Unique sulfated biantennary N-glycans were detected specifically in normal pancreatic islets.					
33581409	2	11	theme	carbohydrate	311:322	arg1	biomarkers					324:333	Current and prospective carbohydrate biomarkers	287:333	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA)	287:412	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	12	12	theme	techniques	2178:2187	arg1	combination					2120:2130	the combination	2116:2130	the combination of lectin-immunohistochemistry and lectin-IMS techniques	2116:2187	Thus, the combination of lectin-immunohistochemistry and lectin-IMS techniques produces more complete information for tumor classification than the individual analyses alone.					
33581409	7	13	from	tissues	1393:1399	arg1	N-glycans					1360:1368	N-glycans	1360:1368	N-glycans from CA19-9-expressing tissues	1360:1399	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	13	14	theme	assessment	2336:2345	arg1	technologies					2347:2358	early assessment technologies	2330:2358	early assessment technologies	2330:2358	These findings potentiate the development of early assessment technologies to rapidly and specifically identify PDAC in the clinic that may directly impact patient outcomes.					
33581409	10	15	theme	selected	1875:1882	arg1	lectins					1884:1890	selected lectins	1875:1890	selected lectins on the same tissues	1875:1910	A candidate new biomarker derived from IMS was further explored by fluorescence staining with selected lectins on the same tissues.					
33581409	1	16	theme	clinical	201:208	arg1	detection					135:143	The early detection	125:143	The early detection of pancreatic ductal adenocarcinoma (PDAC)	125:186	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	1	16	theme	clinical	201:208	arg1	obstacle					210:217	a complex clinical obstacle	191:217	a complex clinical obstacle	191:217	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	1	16	theme	clinical	201:208	arg1	key					226:228	key	226:228	key	226:228	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	7	17	theme	terminal	1491:1498	arg1	residues					1507:1514	both core and terminal fucose residues	1477:1514	residues	1507:1514	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	10	18	theme	new	1793:1795	arg1	biomarker					1797:1805	A candidate new biomarker	1781:1805	A candidate new biomarker derived from IMS	1781:1822	A candidate new biomarker derived from IMS was further explored by fluorescence staining with selected lectins on the same tissues.					
33581409	3	19	theme	imaging	625:631	arg1	spectrometry					638:649	imaging mass spectrometry	625:649	an imaging mass spectrometry (IMS) approach	622:664	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	3	19	theme	imaging	625:631	arg1	IMS					652:654	IMS	652:654	IMS	652:654	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	1	20	theme	survival	277:284	arg1	likelihood					255:264	the overall likelihood	243:264	the overall likelihood of patient survival	243:284	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	11	21	theme	cancer	1969:1974	arg1	cells					1976:1980	cancer cells	1969:1980	cancer cells	1969:1980	The lectins confirmed the expression of the epitopes in cancer cells and revealed different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc.					
33581409	11	22	from	expression	1939:1948	arg1	cells					1976:1980	cancer cells	1969:1980	cancer cells	1969:1980	The lectins confirmed the expression of the epitopes in cancer cells and revealed different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc.					
33581409	1	23	theme	early	129:133	arg1	detection					135:143	The early detection	125:143	The early detection of pancreatic ductal adenocarcinoma (PDAC)	125:186	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	1	23	theme	early	129:133	arg1	obstacle					210:217	a complex clinical obstacle	191:217	a complex clinical obstacle	191:217	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	1	23	theme	early	129:133	arg1	key					226:228	key	226:228	key	226:228	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	8	24	located	detected	1559:1566	arg2	N-glycans					1544:1552	These N-glycans	1538:1552	These N-glycans	1538:1552	These N-glycans were detected in less abundance in sTRA-expressing tumor tissues, which favored triantennary and tetra-antennary structures with polylactosamine extensions.					
33581409	8	24	located	detected	1559:1566	arg1	abundance					1576:1584	less abundance	1571:1584	less abundance	1571:1584	These N-glycans were detected in less abundance in sTRA-expressing tumor tissues, which favored triantennary and tetra-antennary structures with polylactosamine extensions.					
33581409	2	25	dep	biomarkers	324:333	arg1	19-9					356:359	carbohydrate antigen 19-9	335:359	carbohydrate antigen 19-9 (CA19-9)	335:368	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	2	25	dep	biomarkers	324:333	arg1	sTRA					408:411	sTRA	408:411	sTRA	408:411	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	2	25	dep	biomarkers	324:333	arg1	antigen					399:405	sialylated tumor-related antigen	374:405	sialylated tumor-related antigen (sTRA)	374:412	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	2	25	dep	biomarkers	324:333	arg1	CA19-9					362:367	CA19-9	362:367	CA19-9	362:367	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	5	26	theme	distinct	1090:1097	arg1	differences					1099:1109	distinct differences	1090:1109	distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples	1090:1263	These analyses revealed distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples.					
33581409	4	27	theme	carbohydrate	998:1009	arg1	epitopes					1011:1018	carbohydrate epitopes	998:1018	carbohydrate epitopes	998:1018	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	8	28	theme	tumor	1605:1609	arg1	tissues					1611:1617	sTRA-expressing tumor tissues	1589:1617	sTRA-expressing tumor tissues	1589:1617	These N-glycans were detected in less abundance in sTRA-expressing tumor tissues, which favored triantennary and tetra-antennary structures with polylactosamine extensions.					
33581409	7	29	theme	tetra-antennary	1445:1459	arg1	structures					1461:1470	biantennary, triantennary, and tetra-antennary structures	1414:1470	biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues	1414:1514	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	12	30	theme	individual	2258:2267	arg1	analyses					2269:2276	the individual analyses	2254:2276	the individual analyses alone	2254:2282	Thus, the combination of lectin-immunohistochemistry and lectin-IMS techniques produces more complete information for tumor classification than the individual analyses alone.					
33581409	3	31	theme	tissue	792:797	arg1	microarrays					799:809	tissue microarrays	792:809	tissue microarrays	792:809	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	7	32	with	structures	1461:1470	arg1	residues					1507:1514	both core and terminal fucose residues	1477:1514	residues	1507:1514	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	7	32	with	structures	1461:1470	arg1	core					1482:1485	both core and terminal fucose residues	1477:1514	core	1482:1485	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	5	33	theme	healthy	1165:1171	arg1	tissues					1186:1192	both healthy and diseased tissues	1160:1192	tissues	1186:1192	These analyses revealed distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples.					
33581409	3	34	theme	spectrometry	638:649	arg1	approach					657:664	an imaging mass spectrometry (IMS) approach	622:664	an imaging mass spectrometry (IMS) approach	622:664	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	1	35	theme	ductal	159:164	arg1	PDAC					182:185	PDAC	182:185	PDAC	182:185	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	1	35	theme	ductal	159:164	arg1	adenocarcinoma					166:179	pancreatic ductal adenocarcinoma	148:179	pancreatic ductal adenocarcinoma (PDAC)	148:186	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	0	36	from	Spectrometry	13:24	arg1	Tissues					116:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	0	37	theme	Carbohydrate	69:80	arg1	Tissues					116:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	5	38	theme	diseased	1177:1184	arg1	tissues					1186:1192	both healthy and diseased tissues	1160:1192	tissues	1186:1192	These analyses revealed distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples.					
33581409	2	39	theme	noncancerous	540:551	arg1	pathologies					564:574	noncancerous pancreatic pathologies	540:574	noncancerous pancreatic pathologies	540:574	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	0	40	theme	Pancreatic	98:107	arg1	Tissues					116:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	5	41	theme	spatial	1132:1138	arg1	localization					1140:1151	N-glycan spatial localization	1123:1151	N-glycan spatial localization across both healthy and diseased tissues	1123:1192	These analyses revealed distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples.					
33581409	10	42	attach	derived	1807:1813	arg1	IMS					1820:1822	IMS	1820:1822	IMS	1820:1822	A candidate new biomarker derived from IMS was further explored by fluorescence staining with selected lectins on the same tissues.					
33581409	10	42	attach	derived	1807:1813	arg2	biomarker					1797:1805	A candidate new biomarker	1781:1805	A candidate new biomarker derived from IMS	1781:1822	A candidate new biomarker derived from IMS was further explored by fluorescence staining with selected lectins on the same tissues.					
33581409	9	43	theme	Increased	1711:1719	arg1	sialylation					1721:1731	Increased sialylation	1711:1731	Increased sialylation of N-glycans	1711:1744	Increased sialylation of N-glycans was detected in all tumor tissues.					
33581409	8	44	theme	polylactosamine	1683:1697	arg1	extensions					1699:1708	polylactosamine extensions	1683:1708	polylactosamine extensions	1683:1708	These N-glycans were detected in less abundance in sTRA-expressing tumor tissues, which favored triantennary and tetra-antennary structures with polylactosamine extensions.					
33581409	11	45	theme	different	1995:2003	arg1	patterns					2031:2038	different tumor-associated staining patterns	1995:2038	different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc	1995:2107	The lectins confirmed the expression of the epitopes in cancer cells and revealed different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc.					
33581409	2	46	theme	other	512:516	arg1	cancers					528:534	other abdominal cancers	512:534	other abdominal cancers	512:534	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	1	47	theme	patient	269:275	arg1	survival					277:284	patient survival	269:284	patient survival	269:284	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	7	48	theme	triantennary	1427:1438	arg1	structures					1461:1470	biantennary, triantennary, and tetra-antennary structures	1414:1470	biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues	1414:1514	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	2	49	theme	disease	445:451	arg1	progression					453:463	surveilling disease progression	433:463	surveilling disease progression	433:463	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	6	50	theme	sulfated	1273:1280	arg1	N-glycans					1294:1302	Unique sulfated biantennary N-glycans	1266:1302	Unique sulfated biantennary N-glycans	1266:1302	Unique sulfated biantennary N-glycans were detected specifically in normal pancreatic islets.					
33581409	7	51	with	GlcNAc	1530:1535	arg1	residues					1507:1514	both core and terminal fucose residues	1477:1514	residues	1507:1514	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	7	51	with	GlcNAc	1530:1535	arg1	core					1482:1485	both core and terminal fucose residues	1477:1514	core	1482:1485	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	7	52	theme	biantennary	1414:1424	arg1	structures					1461:1470	biantennary, triantennary, and tetra-antennary structures	1414:1470	biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues	1414:1514	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	0	53	theme	Glycans	58:64	arg1	Spectrometry					13:24	Mass Spectrometry	8:24	Mass Spectrometry	8:24	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	0	53	theme	Glycans	58:64	arg1	Analysis					37:44	Lectin Analysis	30:44	Lectin Analysis	30:44	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	11	54	theme	terminal	2093:2100	arg1	GlcNAc					2102:2107	terminal GlcNAc	2093:2107	terminal GlcNAc	2093:2107	The lectins confirmed the expression of the epitopes in cancer cells and revealed different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc.					
33581409	0	55	theme	Lectin	30:35	arg1	Analysis					37:44	Lectin Analysis	30:44	Lectin Analysis	30:44	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	8	56	theme	sTRA-expressing	1589:1603	arg1	tissues					1611:1617	sTRA-expressing tumor tissues	1589:1617	sTRA-expressing tumor tissues	1589:1617	These N-glycans were detected in less abundance in sTRA-expressing tumor tissues, which favored triantennary and tetra-antennary structures with polylactosamine extensions.					
33581409	9	57	gly	sialylation	1721:1731	arg1	N-glycans					1736:1744	N-glycans	1736:1744	N-glycans	1736:1744	Increased sialylation of N-glycans was detected in all tumor tissues.					
33581409	9	57	gly	sialylation	1721:1731	arg1	tissues					1772:1778	all tumor tissues	1762:1778	all tumor tissues	1762:1778	Increased sialylation of N-glycans was detected in all tumor tissues.					
33581409	5	58	from	differences	1099:1109	arg1	localization					1140:1151	N-glycan spatial localization	1123:1151	N-glycan spatial localization across both healthy and diseased tissues	1123:1192	These analyses revealed distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples.					
33581409	11	59	theme	staining	2022:2029	arg1	patterns					2031:2038	different tumor-associated staining patterns	1995:2038	different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc	1995:2107	The lectins confirmed the expression of the epitopes in cancer cells and revealed different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc.					
33581409	9	60	theme	tumor	1766:1770	arg1	tissues					1772:1778	all tumor tissues	1762:1778	all tumor tissues	1762:1778	Increased sialylation of N-glycans was detected in all tumor tissues.					
33581409	12	61	theme	lectin-IMS	2167:2176	arg1	techniques					2178:2187	lectin-immunohistochemistry and lectin-IMS techniques	2135:2187	lectin-immunohistochemistry and lectin-IMS techniques	2135:2187	Thus, the combination of lectin-immunohistochemistry and lectin-IMS techniques produces more complete information for tumor classification than the individual analyses alone.					
33581409	6	62	located	detected	1309:1316	arg1	islets					1352:1357	normal pancreatic islets	1334:1357	normal pancreatic islets	1334:1357	Unique sulfated biantennary N-glycans were detected specifically in normal pancreatic islets.					
33581409	6	62	located	detected	1309:1316	arg2	N-glycans					1294:1302	Unique sulfated biantennary N-glycans	1266:1302	Unique sulfated biantennary N-glycans	1266:1302	Unique sulfated biantennary N-glycans were detected specifically in normal pancreatic islets.					
33581409	2	63	theme	antigen	348:354	arg1	19-9					356:359	carbohydrate antigen 19-9	335:359	carbohydrate antigen 19-9 (CA19-9)	335:368	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	2	63	theme	antigen	348:354	arg1	CA19-9					362:367	CA19-9	362:367	CA19-9	362:367	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	10	64	theme	same	1899:1902	arg1	tissues					1904:1910	the same tissues	1895:1910	the same tissues	1895:1910	A candidate new biomarker derived from IMS was further explored by fluorescence staining with selected lectins on the same tissues.					
33581409	4	65	with	lectins	983:989	arg1	potential					1029:1037	the potential to improve PDAC diagnosis	1025:1063	the potential to improve PDAC diagnosis	1025:1063	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	2	66	theme	sialylated	374:383	arg1	sTRA					408:411	sTRA	408:411	sTRA	408:411	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	2	66	theme	sialylated	374:383	arg1	antigen					399:405	sialylated tumor-related antigen	374:405	sialylated tumor-related antigen (sTRA)	374:412	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	3	67	theme	pancreas	712:719	arg1	N-glycome					689:697	the N-glycome	685:697	the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections	685:835	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	2	68	theme	Current	287:293	arg1	biomarkers					324:333	Current and prospective carbohydrate biomarkers	287:333	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA)	287:412	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	4	69	theme	multiround	893:902	arg1	immunofluorescence					904:921	multiround immunofluorescence	893:921	multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis	893:1063	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	12	70	theme	complete	2203:2210	arg1	information					2212:2222	more complete information	2198:2222	more complete information for tumor classification	2198:2247	Thus, the combination of lectin-immunohistochemistry and lectin-IMS techniques produces more complete information for tumor classification than the individual analyses alone.					
33581409	10	71	from	lectins	1884:1890	arg1	tissues					1904:1910	the same tissues	1895:1910	the same tissues	1895:1910	A candidate new biomarker derived from IMS was further explored by fluorescence staining with selected lectins on the same tissues.					
33581409	3	72	theme	pancreatic	725:734	arg1	cancer					736:741	pancreatic cancer	725:741	pancreatic cancer	725:741	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	6	73	theme	pancreatic	1341:1350	arg1	islets					1352:1357	normal pancreatic islets	1334:1357	normal pancreatic islets	1334:1357	Unique sulfated biantennary N-glycans were detected specifically in normal pancreatic islets.					
33581409	12	74	theme	lectin-immunohistochemistry	2135:2161	arg1	techniques					2178:2187	lectin-immunohistochemistry and lectin-IMS techniques	2135:2187	lectin-immunohistochemistry and lectin-IMS techniques	2135:2187	Thus, the combination of lectin-immunohistochemistry and lectin-IMS techniques produces more complete information for tumor classification than the individual analyses alone.					
33581409	3	75	theme	patients	758:765	arg1	cohort					748:753	a cohort	746:753	a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections	746:835	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	2	76	theme	prospective	299:309	arg1	biomarkers					324:333	Current and prospective carbohydrate biomarkers	287:333	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA)	287:412	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	1	77	theme	complex	193:199	arg1	detection					135:143	The early detection	125:143	The early detection of pancreatic ductal adenocarcinoma (PDAC)	125:186	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	1	77	theme	complex	193:199	arg1	obstacle					210:217	a complex clinical obstacle	191:217	a complex clinical obstacle	191:217	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	1	77	theme	complex	193:199	arg1	key					226:228	key	226:228	key	226:228	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	3	78	theme	glycan	605:610	arg1	epitopes					612:619	these glycan epitopes	599:619	these glycan epitopes	599:619	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	4	79	theme	CA19-9	950:955	arg1	expression					936:945	expression	936:945	expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis	936:1063	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	4	79	theme	CA19-9	950:955	arg1	lectins					983:989	other lectins	977:989	expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis	936:1063	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	10	80	theme	candidate	1783:1791	arg1	biomarker					1797:1805	A candidate new biomarker	1781:1805	A candidate new biomarker derived from IMS	1781:1822	A candidate new biomarker derived from IMS was further explored by fluorescence staining with selected lectins on the same tissues.					
33581409	13	81	theme	technologies	2347:2358	arg1	development					2315:2325	the development	2311:2325	the development of early assessment technologies to rapidly and specifically identify PDAC in the clinic that may directly impact patient outcomes	2311:2456	These findings potentiate the development of early assessment technologies to rapidly and specifically identify PDAC in the clinic that may directly impact patient outcomes.					
33581409	2	82	theme	pancreatic	553:562	arg1	pathologies					564:574	noncancerous pancreatic pathologies	540:574	noncancerous pancreatic pathologies	540:574	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	7	83	theme	fucose	1500:1505	arg1	residues					1507:1514	both core and terminal fucose residues	1477:1514	residues	1507:1514	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	13	84	theme	patient	2441:2447	arg1	outcomes					2449:2456	patient outcomes	2441:2456	patient outcomes	2441:2456	These findings potentiate the development of early assessment technologies to rapidly and specifically identify PDAC in the clinic that may directly impact patient outcomes.					
33581409	5	85	theme	N-glycan	1123:1130	arg1	localization					1140:1151	N-glycan spatial localization	1123:1151	N-glycan spatial localization across both healthy and diseased tissues	1123:1192	These analyses revealed distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples.					
33581409	2	86	from	cancers	528:534	arg1	PDAC					502:505	PDAC	502:505	PDAC from other abdominal cancers and noncancerous pancreatic pathologies	502:574	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	6	87	theme	biantennary	1282:1292	arg1	N-glycans					1294:1302	Unique sulfated biantennary N-glycans	1266:1302	Unique sulfated biantennary N-glycans	1266:1302	Unique sulfated biantennary N-glycans were detected specifically in normal pancreatic islets.					
33581409	3	88	theme	whole-tissue	815:826	arg1	sections					828:835	whole-tissue sections	815:835	whole-tissue sections	815:835	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	8	89	from	abundance	1576:1584	arg1	tissues					1611:1617	sTRA-expressing tumor tissues	1589:1617	sTRA-expressing tumor tissues	1589:1617	These N-glycans were detected in less abundance in sTRA-expressing tumor tissues, which favored triantennary and tetra-antennary structures with polylactosamine extensions.					
33581409	13	90	theme	early	2330:2334	arg1	technologies					2347:2358	early assessment technologies	2330:2358	early assessment technologies	2330:2358	These findings potentiate the development of early assessment technologies to rapidly and specifically identify PDAC in the clinic that may directly impact patient outcomes.					
33581409	3	91	theme	mass	633:636	arg1	spectrometry					638:649	imaging mass spectrometry	625:649	an imaging mass spectrometry (IMS) approach	622:664	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	3	91	theme	mass	633:636	arg1	IMS					652:654	IMS	652:654	IMS	652:654	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	1	92	theme	pancreatic	148:157	arg1	PDAC					182:185	PDAC	182:185	PDAC	182:185	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	1	92	theme	pancreatic	148:157	arg1	adenocarcinoma					166:179	pancreatic ductal adenocarcinoma	148:179	pancreatic ductal adenocarcinoma (PDAC)	148:186	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	6	93	theme	Unique	1266:1271	arg1	N-glycans					1294:1302	Unique sulfated biantennary N-glycans	1266:1302	Unique sulfated biantennary N-glycans	1266:1302	Unique sulfated biantennary N-glycans were detected specifically in normal pancreatic islets.					
33581409	0	94	theme	Antigen-Defined	82:96	arg1	Tissues					116:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	1	95	theme	adenocarcinoma	166:179	arg1	detection					135:143	The early detection	125:143	The early detection of pancreatic ductal adenocarcinoma (PDAC)	125:186	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	1	95	theme	adenocarcinoma	166:179	arg1	obstacle					210:217	a complex clinical obstacle	191:217	a complex clinical obstacle	191:217	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	1	95	theme	adenocarcinoma	166:179	arg1	key					226:228	key	226:228	key	226:228	The early detection of pancreatic ductal adenocarcinoma (PDAC) is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival.					
33581409	8	96	theme	triantennary	1634:1645	arg1	structures					1667:1676	triantennary and tetra-antennary structures	1634:1676	triantennary and tetra-antennary structures	1634:1676	These N-glycans were detected in less abundance in sTRA-expressing tumor tissues, which favored triantennary and tetra-antennary structures with polylactosamine extensions.					
33581409	10	97	theme	fluorescence	1848:1859	arg1	staining					1861:1868	fluorescence staining	1848:1868	fluorescence staining with selected lectins on the same tissues	1848:1910	A candidate new biomarker derived from IMS was further explored by fluorescence staining with selected lectins on the same tissues.					
33581409	0	98	theme	Cancer	109:114	arg1	Tissues					116:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	8	99	theme	tetra-antennary	1651:1665	arg1	structures					1667:1676	triantennary and tetra-antennary structures	1634:1676	triantennary and tetra-antennary structures	1634:1676	These N-glycans were detected in less abundance in sTRA-expressing tumor tissues, which favored triantennary and tetra-antennary structures with polylactosamine extensions.					
33581409	11	100	theme	tumor-associated	2005:2020	arg1	patterns					2031:2038	different tumor-associated staining patterns	1995:2038	different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc	1995:2107	The lectins confirmed the expression of the epitopes in cancer cells and revealed different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc.					
33581409	2	101	theme	abdominal	518:526	arg1	cancers					528:534	other abdominal cancers	512:534	other abdominal cancers	512:534	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	0	102	from	Analysis	37:44	arg1	Tissues					116:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Carbohydrate Antigen-Defined Pancreatic Cancer Tissues	69:122	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	0	103	theme	Mass	8:11	arg1	Spectrometry					13:24	Mass Spectrometry	8:24	Mass Spectrometry	8:24	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	4	104	theme	PDAC	1050:1053	arg1	diagnosis					1055:1063	PDAC diagnosis	1050:1063	PDAC diagnosis	1050:1063	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	2	105	theme	tumor-related	385:397	arg1	sTRA					408:411	sTRA	408:411	sTRA	408:411	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	2	105	theme	tumor-related	385:397	arg1	antigen					399:405	sialylated tumor-related antigen	374:405	sialylated tumor-related antigen (sTRA)	374:412	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	5	106	theme	tissue	1250:1255	arg1	samples					1257:1263	different biomarker-categorized tissue samples	1218:1263	different biomarker-categorized tissue samples	1218:1263	These analyses revealed distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples.					
33581409	3	107	with	patients	758:765	arg1	PDAC					772:775	PDAC	772:775	PDAC represented by tissue microarrays and whole-tissue sections	772:835	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	9	108	theme	N-glycans	1736:1744	arg1	sialylation					1721:1731	Increased sialylation	1711:1731	Increased sialylation of N-glycans	1711:1744	Increased sialylation of N-glycans was detected in all tumor tissues.					
33581409	4	109	with	expression	936:945	arg1	potential					1029:1037	the potential to improve PDAC diagnosis	1025:1063	the potential to improve PDAC diagnosis	1025:1063	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	2	110	theme	surveilling	433:443	arg1	progression					453:463	surveilling disease progression	433:463	surveilling disease progression	433:463	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	3	111	used	used	670:673	arg2	approach					657:664	an imaging mass spectrometry (IMS) approach	622:664	an imaging mass spectrometry (IMS) approach	622:664	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	2	112	gly	sialylated	374:383	arg1	sTRA					408:411	sTRA	408:411	sTRA	408:411	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	2	112	gly	sialylated	374:383	arg1	antigen					399:405	sialylated tumor-related antigen	374:405	sialylated tumor-related antigen (sTRA)	374:412	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	4	113	theme	other	977:981	arg1	lectins					983:989	other lectins	977:989	expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis	936:1063	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	0	114	theme	N-Linked	49:56	arg1	Glycans					58:64	N-Linked Glycans	49:64	N-Linked Glycans	49:64	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.					
33581409	10	115	with	staining	1861:1868	arg1	lectins					1884:1890	selected lectins	1875:1890	selected lectins on the same tissues	1875:1910	A candidate new biomarker derived from IMS was further explored by fluorescence staining with selected lectins on the same tissues.					
33581409	7	116	theme	CA19-9-expressing	1375:1391	arg1	tissues					1393:1399	CA19-9-expressing tissues	1375:1399	CA19-9-expressing tissues	1375:1399	N-glycans from CA19-9-expressing tissues tended to be biantennary, triantennary, and tetra-antennary structures with both core and terminal fucose residues and bisecting GlcNAc.					
33581409	4	117	theme	sTRA	961:964	arg1	expression					936:945	expression	936:945	expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis	936:1063	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	4	117	theme	sTRA	961:964	arg1	lectins					983:989	other lectins	977:989	expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis	936:1063	Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis.					
33581409	5	118	theme	different	1218:1226	arg1	samples					1257:1263	different biomarker-categorized tissue samples	1218:1263	different biomarker-categorized tissue samples	1218:1263	These analyses revealed distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples.					
33581409	2	119	from	pathologies	564:574	arg1	PDAC					502:505	PDAC	502:505	PDAC from other abdominal cancers and noncancerous pancreatic pathologies	502:574	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	2	120	theme	carbohydrate	335:346	arg1	19-9					356:359	carbohydrate antigen 19-9	335:359	carbohydrate antigen 19-9 (CA19-9)	335:368	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	2	120	theme	carbohydrate	335:346	arg1	CA19-9					362:367	CA19-9	362:367	CA19-9	362:367	Current and prospective carbohydrate biomarkers carbohydrate antigen 19-9 (CA19-9) and sialylated tumor-related antigen (sTRA) are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and noncancerous pancreatic pathologies.					
33581409	12	121	theme	tumor	2228:2232	arg1	classification					2234:2247	tumor classification	2228:2247	tumor classification	2228:2247	Thus, the combination of lectin-immunohistochemistry and lectin-IMS techniques produces more complete information for tumor classification than the individual analyses alone.					
33581409	3	122	theme	human	706:710	arg1	pancreas					712:719	the human pancreas	702:719	the human pancreas	702:719	To further understand these glycan epitopes, an imaging mass spectrometry (IMS) approach was used to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of patients with PDAC represented by tissue microarrays and whole-tissue sections.					
33581409	11	123	theme	bisecting	2061:2069	arg1	GlcNAc					2071:2076	bisecting GlcNAc	2061:2076	bisecting GlcNAc	2061:2076	The lectins confirmed the expression of the epitopes in cancer cells and revealed different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc.					
34495537	8	0	theme	immune	1381:1386	arg1	proteins					1388:1395	serum immune proteins	1375:1395	serum immune proteins	1375:1395	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	8	0	theme	immune	1381:1386	arg1	IgG					1408:1410	IgG	1408:1410	IgG in autoimmunity	1408:1426	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	2	1	theme	lupus	337:341	arg1	SLE					358:360	SLE	358:360	SLE	358:360	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	2	1	theme	lupus	337:341	arg1	erythematosus					343:355	systemic lupus erythematosus	328:355	systemic lupus erythematosus (SLE)	328:361	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	2	2	from	alteration	174:183	arg1	autoimmunity					188:199	autoimmunity	188:199	autoimmunity	188:199	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	8	3	theme	serum	1375:1379	arg1	proteins					1388:1395	serum immune proteins	1375:1395	serum immune proteins	1375:1395	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	8	3	theme	serum	1375:1379	arg1	IgG					1408:1410	IgG	1408:1410	IgG in autoimmunity	1408:1426	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	3	4	theme	N-glycans	449:457	arg1	rebuilding					431:440	The rebuilding	427:440	The rebuilding of IgG N-glycans in RA	427:463	The rebuilding of IgG N-glycans in RA correlates with the relapses and remissions of the disease, is associated with physiological states such as pregnancy but also depends on applied anti-inflammatory therapy.					
34495537	4	5	gly	fucosylation	664:675	arg1	pool					690:693	the whole pool	680:693	the whole pool of IgG N-glycans	680:710	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	0	6	from	Glycosylation	0:12	arg1	Diseases					28:35	Autoimmune Diseases	17:35	Autoimmune Diseases	17:35	Glycosylation in Autoimmune Diseases.					
34495537	2	7	theme	G	239:239	arg1	agalactosylation					204:219	agalactosylation	204:219	agalactosylation	204:219	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	4	8	theme	pool	690:693	arg1	fucosylation					664:675	a decreased core fucosylation	647:675	a decreased core fucosylation of the whole pool of IgG N-glycans	647:710	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	4	8	theme	pool	690:693	arg1	glycomarker					723:733	a serum glycomarker	715:733	a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD)	715:838	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	7	9	theme	gene	1112:1115	arg1	expression					1092:1101	the reduced expression	1080:1101	the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation	1080:1266	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	3	10	theme	IgG	445:447	arg1	N-glycans					449:457	IgG N-glycans	445:457	IgG N-glycans	445:457	The rebuilding of IgG N-glycans in RA correlates with the relapses and remissions of the disease, is associated with physiological states such as pregnancy but also depends on applied anti-inflammatory therapy.					
34495537	4	11	theme	decreased	649:657	arg1	fucosylation					664:675	a decreased core fucosylation	647:675	a decreased core fucosylation of the whole pool of IgG N-glycans	647:710	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	4	11	theme	decreased	649:657	arg1	glycomarker					723:733	a serum glycomarker	715:733	a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD)	715:838	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	7	12	theme	inflammation	1046:1057	arg1	lymphocytes					1061:1071	chronic inflammation T lymphocytes	1038:1071	chronic inflammation T lymphocytes	1038:1071	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	6	13	theme	oligosaccharides	972:987	arg1	fucosylation					952:963	Core fucosylation	947:963	Core fucosylation of IgG oligosaccharides	947:987	Core fucosylation of IgG oligosaccharides was also lowered in MS and SLE.					
34495537	2	14	theme	rheumatoid	275:284	arg1	arthritis					286:294	rheumatoid arthritis	275:294	rheumatoid arthritis (RA)	275:299	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	2	14	theme	rheumatoid	275:284	arg1	RA					297:298	RA	297:298	RA	297:298	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	7	15	theme	N-glycans	1207:1215	arg1	β1,6-branching					1189:1202	β1,6-branching	1189:1202	β1,6-branching	1189:1202	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	2	16	theme	inflammatory	364:375	arg1	disease					383:389	inflammatory bowel disease	364:389	inflammatory bowel disease (IBD)	364:395	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	2	16	theme	inflammatory	364:375	arg1	IBD					392:394	IBD	392:394	IBD	392:394	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	7	17	theme	T	1059:1059	arg1	lymphocytes					1061:1071	chronic inflammation T lymphocytes	1038:1071	chronic inflammation T lymphocytes	1038:1071	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	4	18	theme	IgG	698:700	arg1	N-glycans					702:710	IgG N-glycans	698:710	IgG N-glycans	698:710	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	6	19	theme	IgG	968:970	arg1	oligosaccharides					972:987	IgG oligosaccharides	968:987	IgG oligosaccharides	968:987	Core fucosylation of IgG oligosaccharides was also lowered in MS and SLE.					
34495537	2	20	theme	multiple	402:409	arg1	MS					422:423	MS	422:423	MS	422:423	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	2	20	theme	multiple	402:409	arg1	sclerosis					411:419	multiple sclerosis	402:419	multiple sclerosis (MS)	402:424	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	7	21	theme	reduced	1084:1090	arg1	expression					1092:1101	the reduced expression	1080:1101	the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation	1080:1266	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	7	22	theme	β1,6-N-acetylglucosaminyltransferase	1126:1161	arg1	GnT-V					1166:1170	GnT-V	1166:1170	GnT-V	1166:1170	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	7	22	theme	β1,6-N-acetylglucosaminyltransferase	1126:1161	arg1	V					1163:1163	β1,6-N-acetylglucosaminyltransferase V	1126:1163	β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation	1126:1266	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	1	23	theme	protein	88:94	arg1	glycosylation					96:108	protein glycosylation	88:108	protein glycosylation	88:108	Autoimmune diseases are accompanied by changes in protein glycosylation, in both the immune system and target tissues.					
34495537	6	24	theme	Core	947:950	arg1	fucosylation					952:963	Core fucosylation	947:963	Core fucosylation of IgG oligosaccharides	947:987	Core fucosylation of IgG oligosaccharides was also lowered in MS and SLE.					
34495537	7	25	theme	chronic	1038:1044	arg1	inflammation					1046:1057	chronic inflammation	1038:1057	chronic inflammation T lymphocytes	1038:1071	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	7	26	theme	MGAT5	1106:1110	arg1	gene					1112:1115	MGAT5 gene	1106:1115	MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation	1106:1266	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	3	27	from	rebuilding	431:440	arg1	RA					462:463	RA	462:463	RA	462:463	The rebuilding of IgG N-glycans in RA correlates with the relapses and remissions of the disease, is associated with physiological states such as pregnancy but also depends on applied anti-inflammatory therapy.					
34495537	8	28	theme	cells	1365:1369	arg1	activity					1346:1353	the pro-inflammatory activity	1325:1353	the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity	1325:1426	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	7	29	theme	receptor	1248:1255	arg1	activation					1257:1266	T cell receptor activation	1241:1266	T cell receptor activation	1241:1266	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	4	30	theme	thyroid	749:755	arg1	AITD					767:770	AITD	767:770	AITD	767:770	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	4	30	theme	thyroid	749:755	arg1	diseases					757:764	autoimmune thyroid diseases	738:764	autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD)	738:838	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	4	31	theme	whole	684:688	arg1	pool					690:693	the whole pool	680:693	the whole pool of IgG N-glycans	680:710	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	0	32	theme	Autoimmune	17:26	arg1	Diseases					28:35	Autoimmune Diseases	17:35	Autoimmune Diseases	17:35	Glycosylation in Autoimmune Diseases.					
34495537	4	33	theme	N-glycans	702:710	arg1	pool					690:693	the whole pool	680:693	the whole pool of IgG N-glycans	680:710	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	8	34	theme	pro-inflammatory	1329:1344	arg1	activity					1346:1353	the pro-inflammatory activity	1325:1353	the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity	1325:1426	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	2	35	theme	systemic	328:335	arg1	SLE					358:360	SLE	358:360	SLE	358:360	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	2	35	theme	systemic	328:335	arg1	erythematosus					343:355	systemic lupus erythematosus	328:355	systemic lupus erythematosus (SLE)	328:361	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	7	36	theme	cell	1243:1246	arg1	activation					1257:1266	T cell receptor activation	1241:1266	T cell receptor activation	1241:1266	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	4	37	theme	autoimmune	738:747	arg1	AITD					767:770	AITD	767:770	AITD	767:770	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	4	37	theme	autoimmune	738:747	arg1	diseases					757:764	autoimmune thyroid diseases	738:764	autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD)	738:838	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	7	38	theme	responsible	1173:1183	arg1	GnT-V					1166:1170	GnT-V	1166:1170	GnT-V	1166:1170	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	7	38	theme	responsible	1173:1183	arg1	V					1163:1163	β1,6-N-acetylglucosaminyltransferase V	1126:1163	β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation	1126:1266	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	8	39	contain	have	1299:1302	arg1	changes					1280:1286	Structural changes	1269:1286	Structural changes of glycans	1269:1297	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	8	39	contain	have	1299:1302	arg2	effect					1315:1320	a profound effect	1304:1320	a profound effect	1304:1320	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	2	40	theme	best-studied	161:172	arg1	alteration					174:183	The best-studied alteration	157:183	The best-studied alteration in autoimmunity	157:199	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	5	41	theme	HT	917:918	arg1	marker					907:912	an immunological marker	890:912	an immunological marker of HT	890:918	However, fucosylation of anti-thyroglobulin IgG (an immunological marker of HT) was elevated in HT serum.					
34495537	2	42	theme	bowel	377:381	arg1	disease					383:389	inflammatory bowel disease	364:389	inflammatory bowel disease (IBD)	364:395	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	2	42	theme	bowel	377:381	arg1	IBD					392:394	IBD	392:394	IBD	392:394	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	6	43	gly	fucosylation	952:963	arg1	SLE					1016:1018	SLE	1016:1018	SLE	1016:1018	Core fucosylation of IgG oligosaccharides was also lowered in MS and SLE.					
34495537	6	43	gly	fucosylation	952:963	arg1	MS					1009:1010	MS	1009:1010	MS	1009:1010	Core fucosylation of IgG oligosaccharides was also lowered in MS and SLE.					
34495537	6	43	gly	fucosylation	952:963	arg1	oligosaccharides					972:987	IgG oligosaccharides	968:987	IgG oligosaccharides	968:987	Core fucosylation of IgG oligosaccharides was also lowered in MS and SLE.					
34495537	3	44	theme	disease	516:522	arg1	remissions					498:507	remissions	498:507	remissions	498:507	The rebuilding of IgG N-glycans in RA correlates with the relapses and remissions of the disease, is associated with physiological states such as pregnancy but also depends on applied anti-inflammatory therapy.					
34495537	3	44	theme	disease	516:522	arg1	relapses					485:492	relapses	485:492	relapses	485:492	The rebuilding of IgG N-glycans in RA correlates with the relapses and remissions of the disease, is associated with physiological states such as pregnancy but also depends on applied anti-inflammatory therapy.					
34495537	8	45	from	IgG	1408:1410	arg1	autoimmunity					1415:1426	autoimmunity	1415:1426	autoimmunity	1415:1426	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	3	46	theme	applied	603:609	arg1	therapy					629:635	applied anti-inflammatory therapy	603:635	applied anti-inflammatory therapy	603:635	The rebuilding of IgG N-glycans in RA correlates with the relapses and remissions of the disease, is associated with physiological states such as pregnancy but also depends on applied anti-inflammatory therapy.					
34495537	2	47	theme	immunoglobulin	224:237	arg1	IgG					242:244	IgG	242:244	IgG	242:244	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	2	47	theme	immunoglobulin	224:237	arg1	G					239:239	immunoglobulin G	224:239	immunoglobulin G (IgG)	224:245	The best-studied alteration in autoimmunity is agalactosylation of immunoglobulin G (IgG), characterized primarily in rheumatoid arthritis (RA), and then detected also in systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), and multiple sclerosis (MS).					
34495537	5	48	dep	IgG	885:887	arg1	marker					907:912	an immunological marker	890:912	an immunological marker of HT	890:918	However, fucosylation of anti-thyroglobulin IgG (an immunological marker of HT) was elevated in HT serum.					
34495537	8	49	theme	profound	1306:1313	arg1	effect					1315:1320	a profound effect	1304:1320	a profound effect	1304:1320	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	4	50	theme	serum	717:721	arg1	fucosylation					664:675	a decreased core fucosylation	647:675	a decreased core fucosylation of the whole pool of IgG N-glycans	647:710	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	4	50	theme	serum	717:721	arg1	glycomarker					723:733	a serum glycomarker	715:733	a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD)	715:838	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	8	51	theme	immune	1358:1363	arg1	cells					1365:1369	immune cells	1358:1369	immune cells	1358:1369	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	8	51	theme	immune	1358:1363	arg1	IgG					1408:1410	IgG	1408:1410	IgG in autoimmunity	1408:1426	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	3	52	theme	anti-inflammatory	611:627	arg1	therapy					629:635	applied anti-inflammatory therapy	603:635	applied anti-inflammatory therapy	603:635	The rebuilding of IgG N-glycans in RA correlates with the relapses and remissions of the disease, is associated with physiological states such as pregnancy but also depends on applied anti-inflammatory therapy.					
34495537	7	53	theme	T	1241:1241	arg1	activation					1257:1266	T cell receptor activation	1241:1266	T cell receptor activation	1241:1266	In AITD and IBD, chronic inflammation T lymphocytes showed the reduced expression of MGAT5 gene encoding β1,6-N-acetylglucosaminyltransferase V (GnT-V) responsible for β1,6-branching of N-glycans, which is important for T cell receptor activation.					
34495537	1	54	theme	immune	123:128	arg1	system					130:135	the immune system	119:135	the immune system	119:135	Autoimmune diseases are accompanied by changes in protein glycosylation, in both the immune system and target tissues.					
34495537	8	55	theme	Structural	1269:1278	arg1	changes					1280:1286	Structural changes	1269:1286	Structural changes of glycans	1269:1297	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	5	56	theme	IgG	885:887	arg1	fucosylation					850:861	fucosylation	850:861	fucosylation of anti-thyroglobulin IgG (an immunological marker of HT)	850:919	However, fucosylation of anti-thyroglobulin IgG (an immunological marker of HT) was elevated in HT serum.					
34495537	5	57	theme	HT	937:938	arg1	serum					940:944	HT serum	937:944	HT serum	937:944	However, fucosylation of anti-thyroglobulin IgG (an immunological marker of HT) was elevated in HT serum.					
34495537	1	58	theme	Autoimmune	38:47	arg1	diseases					49:56	Autoimmune diseases	38:56	Autoimmune diseases	38:56	Autoimmune diseases are accompanied by changes in protein glycosylation, in both the immune system and target tissues.					
34495537	4	59	from	glycomarker	723:733	arg1	turn					641:644	turn	641:644	turn	641:644	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	4	59	from	glycomarker	723:733	arg1	AITD					767:770	AITD	767:770	AITD	767:770	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	4	59	from	glycomarker	723:733	arg1	diseases					757:764	autoimmune thyroid diseases	738:764	autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD)	738:838	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	3	60	dep	relapses	485:492	arg1	the					481:483	the	481:483	the	481:483	The rebuilding of IgG N-glycans in RA correlates with the relapses and remissions of the disease, is associated with physiological states such as pregnancy but also depends on applied anti-inflammatory therapy.					
34495537	4	61	theme	core	659:662	arg1	fucosylation					664:675	a decreased core fucosylation	647:675	a decreased core fucosylation of the whole pool of IgG N-glycans	647:710	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	4	61	theme	core	659:662	arg1	glycomarker					723:733	a serum glycomarker	715:733	a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD)	715:838	In turn, a decreased core fucosylation of the whole pool of IgG N-glycans is a serum glycomarker in autoimmune thyroid diseases (AITD) encompassing Hashimoto's thyroiditis (HT) and Grave's disease (GD).					
34495537	5	62	theme	anti-thyroglobulin	866:883	arg1	IgG					885:887	anti-thyroglobulin IgG	866:887	anti-thyroglobulin IgG (an immunological marker of HT)	866:919	However, fucosylation of anti-thyroglobulin IgG (an immunological marker of HT) was elevated in HT serum.					
34495537	5	63	theme	immunological	893:905	arg1	marker					907:912	an immunological marker	890:912	an immunological marker of HT	890:918	However, fucosylation of anti-thyroglobulin IgG (an immunological marker of HT) was elevated in HT serum.					
34495537	8	64	theme	glycans	1291:1297	arg1	changes					1280:1286	Structural changes	1269:1286	Structural changes of glycans	1269:1297	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	5	65	gly	fucosylation	850:861	arg1	serum					940:944	HT serum	937:944	HT serum	937:944	However, fucosylation of anti-thyroglobulin IgG (an immunological marker of HT) was elevated in HT serum.					
34495537	5	65	gly	fucosylation	850:861	arg1	IgG					885:887	anti-thyroglobulin IgG	866:887	anti-thyroglobulin IgG (an immunological marker of HT)	866:919	However, fucosylation of anti-thyroglobulin IgG (an immunological marker of HT) was elevated in HT serum.					
34495537	8	66	theme	proteins	1388:1395	arg1	activity					1346:1353	the pro-inflammatory activity	1325:1353	the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity	1325:1426	Structural changes of glycans have a profound effect on the pro-inflammatory activity of immune cells and serum immune proteins, including IgG in autoimmunity.					
34495537	3	67	theme	physiological	544:556	arg1	states					558:563	physiological states	544:563	physiological states such as pregnancy	544:581	The rebuilding of IgG N-glycans in RA correlates with the relapses and remissions of the disease, is associated with physiological states such as pregnancy but also depends on applied anti-inflammatory therapy.					
34495537	3	67	theme	physiological	544:556	arg1	pregnancy					573:581	pregnancy	573:581	pregnancy	573:581	The rebuilding of IgG N-glycans in RA correlates with the relapses and remissions of the disease, is associated with physiological states such as pregnancy but also depends on applied anti-inflammatory therapy.					
34495537	1	68	theme	target	141:146	arg1	tissues					148:154	target tissues	141:154	target tissues	141:154	Autoimmune diseases are accompanied by changes in protein glycosylation, in both the immune system and target tissues.					
34495537	1	69	from	changes	77:83	arg1	glycosylation					96:108	protein glycosylation	88:108	protein glycosylation	88:108	Autoimmune diseases are accompanied by changes in protein glycosylation, in both the immune system and target tissues.					
34495537	1	69	from	changes	77:83	arg1	system					130:135	the immune system	119:135	the immune system	119:135	Autoimmune diseases are accompanied by changes in protein glycosylation, in both the immune system and target tissues.					
34495537	1	69	from	changes	77:83	arg1	tissues					148:154	target tissues	141:154	target tissues	141:154	Autoimmune diseases are accompanied by changes in protein glycosylation, in both the immune system and target tissues.					
33141553	7	0	theme	compositional	1413:1425	arg1	findings					1427:1434	compositional findings	1413:1434	compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera	1413:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	3	1	theme	native	540:545	arg1	protein					547:553	the native protein	536:553	the native protein	536:553	Unlike hereditary TTR (ATTRm) amyloidosis, where amino acid replacements destabilize the native protein, in ATTRwt amyloidosis, amyloid-forming TTR lacks protein sequence alterations.					
33141553	7	2	from	analyses	1459:1466	arg1	detail					1403:1408	comprehensive detail	1389:1408	comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera	1389:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	7	2	from	analyses	1459:1466	arg1	findings					1427:1434	compositional findings	1413:1434	compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera	1413:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	8	3	theme	oligosaccharide	1571:1585	arg1	content					1587:1593	oligosaccharide content	1571:1593	oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis	1571:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	6	4	theme	ATTRwt	1150:1155	arg1	tissue					1165:1170	ATTRwt cardiac tissue	1150:1170	ATTRwt cardiac tissue	1150:1170	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	3	5	theme	ATTRwt	559:564	arg1	amyloidosis					566:576	ATTRwt amyloidosis	559:576	ATTRwt amyloidosis	559:576	Unlike hereditary TTR (ATTRm) amyloidosis, where amino acid replacements destabilize the native protein, in ATTRwt amyloidosis, amyloid-forming TTR lacks protein sequence alterations.					
33141553	8	6	theme	novel	1657:1661	arg1	characterization					1551:1566	The characterization	1547:1566	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis	1547:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	8	6	theme	novel	1657:1661	arg1	data					1663:1666	novel data	1657:1666	novel data	1657:1666	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	9	7	from	role	1815:1818	arg1	pathobiology					1837:1848	ATTRwt pathobiology	1830:1848	ATTRwt pathobiology	1830:1848	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	6	8	theme	non-native	1201:1210	arg1	forms					1239:1243	non-native (monomeric and aggregated) forms	1201:1243	non-native (monomeric and aggregated) forms of TTR	1201:1250	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	2	9	theme	amyloid	346:352	arg1	deposits					361:368	cardiac amyloid fibril deposits	338:368	cardiac amyloid fibril deposits that originate from dissociation of the tetrameric protein, transthyretin (TTR)	338:448	This disease frequently features cardiac amyloid fibril deposits that originate from dissociation of the tetrameric protein, transthyretin (TTR).					
33141553	9	10	theme	structural	1887:1896	arg1	features					1898:1905	disease-specific structural features	1870:1905	disease-specific structural features that limit the chaperoning capacity of CLU	1870:1948	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	6	11	theme	CLU	1261:1263	arg1	levels					1271:1276	(3) CLU serum levels	1257:1276	(3) CLU serum levels in patients with ATTRwt	1257:1300	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	3	12	theme	TTR	469:471	arg1	amyloidosis					481:491	hereditary TTR (ATTRm) amyloidosis	458:491	hereditary TTR (ATTRm) amyloidosis	458:491	Unlike hereditary TTR (ATTRm) amyloidosis, where amino acid replacements destabilize the native protein, in ATTRwt amyloidosis, amyloid-forming TTR lacks protein sequence alterations.					
33141553	1	13	theme	age-related	242:252	arg1	disease					254:260	an age-related disease	239:260	an age-related disease that causes heart failure in older adults	239:302	Wild-type transthyretin-associated (ATTRwt) amyloidosis is an age-related disease that causes heart failure in older adults.					
33141553	1	13	theme	age-related	242:252	arg1	amyloidosis					224:234	Wild-type transthyretin-associated (ATTRwt) amyloidosis	180:234	Wild-type transthyretin-associated (ATTRwt) amyloidosis	180:234	Wild-type transthyretin-associated (ATTRwt) amyloidosis is an age-related disease that causes heart failure in older adults.					
33141553	0	14	theme	Spectrometry	157:168	arg1	Analyses					170:177	Mass Spectrometry Analyses	152:177	Mass Spectrometry Analyses	152:177	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	7	15	theme	control	1533:1539	arg1	sera					1541:1544	control sera	1533:1544	control sera	1533:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	5	16	gly	glycoprotein	916:927	arg1	clusterin					860:868	clusterin	860:868	clusterin (CLU, UniProtKB P10909)	860:892	We believe that clusterin (CLU, UniProtKB P10909), a plasma circulating glycoprotein, plays a role in the pathobiology of ATTRwt amyloidosis.					
33141553	5	16	gly	glycoprotein	916:927	arg1	glycoprotein					916:927	a plasma circulating glycoprotein	895:927	a plasma circulating glycoprotein	895:927	We believe that clusterin (CLU, UniProtKB P10909), a plasma circulating glycoprotein, plays a role in the pathobiology of ATTRwt amyloidosis.					
33141553	6	17	theme	TTR	1248:1250	arg1	forms					1239:1243	non-native (monomeric and aggregated) forms	1201:1243	non-native (monomeric and aggregated) forms of TTR	1201:1250	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	9	18	theme	CLU	1946:1948	arg1	capacity					1934:1941	the chaperoning capacity	1918:1941	the chaperoning capacity of CLU	1918:1948	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	0	19	theme	ATTRwt	72:77	arg1	Amyloidosis					80:90	Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis	36:90	Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis	36:90	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	5	20	theme	plasma	897:902	arg1	clusterin					860:868	clusterin	860:868	clusterin (CLU, UniProtKB P10909)	860:892	We believe that clusterin (CLU, UniProtKB P10909), a plasma circulating glycoprotein, plays a role in the pathobiology of ATTRwt amyloidosis.					
33141553	5	20	theme	plasma	897:902	arg1	glycoprotein					916:927	a plasma circulating glycoprotein	895:927	a plasma circulating glycoprotein	895:927	We believe that clusterin (CLU, UniProtKB P10909), a plasma circulating glycoprotein, plays a role in the pathobiology of ATTRwt amyloidosis.					
33141553	9	21	theme	CLU	1697:1699	arg1	variations					1717:1726	CLU oligosaccharide variations	1697:1726	CLU oligosaccharide variations between patient and healthy controls	1697:1763	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	6	22	from	role	1018:1021	arg1	amyloidosis					1041:1051	ATTRwt amyloidosis	1034:1051	ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls	1034:1353	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	6	23	dep	levels	1271:1276	arg1	3					1258:1258	3	1258:1258	3	1258:1258	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	1	24	theme	Wild-type	180:188	arg1	ATTRwt					216:221	ATTRwt	216:221	ATTRwt	216:221	Wild-type transthyretin-associated (ATTRwt) amyloidosis is an age-related disease that causes heart failure in older adults.					
33141553	1	24	theme	Wild-type	180:188	arg1	disease					254:260	an age-related disease	239:260	an age-related disease that causes heart failure in older adults	239:302	Wild-type transthyretin-associated (ATTRwt) amyloidosis is an age-related disease that causes heart failure in older adults.					
33141553	1	24	theme	Wild-type	180:188	arg1	amyloidosis					224:234	Wild-type transthyretin-associated (ATTRwt) amyloidosis	180:234	Wild-type transthyretin-associated (ATTRwt) amyloidosis	180:234	Wild-type transthyretin-associated (ATTRwt) amyloidosis is an age-related disease that causes heart failure in older adults.					
33141553	0	25	from	Glycosylation	0:12	arg1	Amyloidosis					80:90	Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis	36:90	Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis	36:90	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	8	26	from	CLU	1604:1606	arg1	characterization					1551:1566	The characterization	1547:1566	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis	1547:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	8	26	from	CLU	1604:1606	arg1	data					1663:1666	novel data	1657:1666	novel data	1657:1666	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	7	27	theme	present	1363:1369	arg1	study					1371:1375	the present study	1359:1375	the present study	1359:1375	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	5	28	theme	amyloidosis	973:983	arg1	pathobiology					950:961	the pathobiology	946:961	the pathobiology of ATTRwt amyloidosis	946:983	We believe that clusterin (CLU, UniProtKB P10909), a plasma circulating glycoprotein, plays a role in the pathobiology of ATTRwt amyloidosis.					
33141553	7	29	theme	glycan	1486:1491	arg1	content					1493:1499	glycan content	1486:1499	glycan content of CLU purified from ATTRwt and control sera	1486:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	9	30	theme	healthy	1748:1754	arg1	controls					1756:1763	healthy controls	1748:1763	healthy controls	1748:1763	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	3	31	theme	hereditary	458:467	arg1	amyloidosis					481:491	hereditary TTR (ATTRm) amyloidosis	458:491	hereditary TTR (ATTRm) amyloidosis	458:491	Unlike hereditary TTR (ATTRm) amyloidosis, where amino acid replacements destabilize the native protein, in ATTRwt amyloidosis, amyloid-forming TTR lacks protein sequence alterations.					
33141553	4	32	theme	TTR	774:776	arg1	misfolding					778:787	TTR misfolding	774:787	TTR misfolding	774:787	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	6	33	from	TTR	1122:1124	arg1	fibrils					1137:1143	amyloid fibrils	1129:1143	amyloid fibrils from ATTRwt cardiac tissue	1129:1170	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	6	33	from	TTR	1122:1124	arg1	tissue					1165:1170	ATTRwt cardiac tissue	1150:1170	ATTRwt cardiac tissue	1150:1170	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	0	34	theme	Serum	17:21	arg1	Clusterin					23:31	Serum Clusterin	17:31	Serum Clusterin	17:31	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	7	35	theme	amino	1471:1475	arg1	acid					1477:1480	amino acid	1471:1480	amino acid	1471:1480	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	6	36	theme	healthy	1338:1344	arg1	controls					1346:1353	healthy controls	1338:1353	healthy controls	1338:1353	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	9	37	theme	further	1790:1796	arg1	evidence					1798:1805	further evidence	1790:1805	further evidence for the role of CLU in ATTRwt pathobiology	1790:1848	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	0	38	theme	Transthyretin-Associated	46:69	arg1	Amyloidosis					80:90	Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis	36:90	Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis	36:90	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	7	39	theme	mass	1441:1444	arg1	analyses					1459:1466	mass spectrometry analyses	1441:1466	mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera	1441:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	6	40	dep	codeposits	1095:1104	arg1	1					1088:1088	1	1088:1088	1	1088:1088	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	3	41	theme	amino	500:504	arg1	replacements					511:522	amino acid replacements	500:522	amino acid replacements	500:522	Unlike hereditary TTR (ATTRm) amyloidosis, where amino acid replacements destabilize the native protein, in ATTRwt amyloidosis, amyloid-forming TTR lacks protein sequence alterations.					
33141553	8	42	theme	content	1587:1593	arg1	characterization					1551:1566	The characterization	1547:1566	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis	1547:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	8	42	theme	content	1587:1593	arg1	data					1663:1666	novel data	1657:1666	novel data	1657:1666	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	7	43	theme	findings	1427:1434	arg1	detail					1403:1408	comprehensive detail	1389:1408	comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera	1389:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	6	44	theme	CLU	1091:1093	arg1	codeposits					1095:1104	(1) CLU codeposits	1087:1104	(1) CLU codeposits	1087:1104	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	4	45	theme	initiating	639:648	arg1	cause					650:654	The initiating cause	635:654	The initiating cause of fibril formation in ATTRwt amyloidosis	635:696	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	4	45	theme	initiating	639:648	arg1	unclear					701:707	unclear	701:707	unclear	701:707	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	4	46	theme	fibrils	835:841	arg1	misfolding					778:787	TTR misfolding	774:787	TTR misfolding	774:787	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	4	46	theme	fibrils	835:841	arg1	accumulation					805:816	unregulated accumulation	793:816	unregulated accumulation	793:816	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	2	47	theme	tetrameric	410:419	arg1	transthyretin					430:442	transthyretin	430:442	transthyretin (TTR)	430:448	This disease frequently features cardiac amyloid fibril deposits that originate from dissociation of the tetrameric protein, transthyretin (TTR).					
33141553	2	47	theme	tetrameric	410:419	arg1	protein					421:427	the tetrameric protein	406:427	the tetrameric protein	406:427	This disease frequently features cardiac amyloid fibril deposits that originate from dissociation of the tetrameric protein, transthyretin (TTR).					
33141553	8	48	theme	serum	1598:1602	arg1	CLU					1604:1606	serum CLU	1598:1606	serum CLU derived from patients with ATTRwt amyloidosis	1598:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	6	49	dep	non-native	1201:1210	arg1	monomeric					1213:1221	monomeric	1213:1221	monomeric	1213:1221	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	6	49	dep	non-native	1201:1210	arg1	aggregated					1227:1236	aggregated	1227:1236	aggregated	1227:1236	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	6	50	with	codeposits	1095:1104	arg1	TTR					1122:1124	non-native TTR	1111:1124	non-native TTR in amyloid fibrils from ATTRwt cardiac tissue	1111:1170	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	0	51	dep	Glycosylation	0:12	arg1	Study					95:99	A Study	93:99	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.	0:178	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	9	52	theme	CLU	1823:1825	arg1	role					1815:1818	the role	1811:1818	the role of CLU in ATTRwt pathobiology	1811:1848	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	7	53	theme	comprehensive	1389:1401	arg1	detail					1403:1408	comprehensive detail	1389:1408	comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera	1389:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	9	54	theme	ATTRwt	1830:1835	arg1	pathobiology					1837:1848	ATTRwt pathobiology	1830:1848	ATTRwt pathobiology	1830:1848	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	1	55	theme	older	291:295	arg1	adults					297:302	older adults	291:302	older adults	291:302	Wild-type transthyretin-associated (ATTRwt) amyloidosis is an age-related disease that causes heart failure in older adults.					
33141553	6	56	theme	cardiac	1157:1163	arg1	tissue					1165:1170	ATTRwt cardiac tissue	1150:1170	ATTRwt cardiac tissue	1150:1170	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	8	57	theme	ATTRwt	1635:1640	arg1	amyloidosis					1642:1652	ATTRwt amyloidosis	1635:1652	ATTRwt amyloidosis	1635:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	1	58	theme	transthyretin-associated	190:213	arg1	ATTRwt					216:221	ATTRwt	216:221	ATTRwt	216:221	Wild-type transthyretin-associated (ATTRwt) amyloidosis is an age-related disease that causes heart failure in older adults.					
33141553	1	58	theme	transthyretin-associated	190:213	arg1	disease					254:260	an age-related disease	239:260	an age-related disease that causes heart failure in older adults	239:302	Wild-type transthyretin-associated (ATTRwt) amyloidosis is an age-related disease that causes heart failure in older adults.					
33141553	1	58	theme	transthyretin-associated	190:213	arg1	amyloidosis					224:234	Wild-type transthyretin-associated (ATTRwt) amyloidosis	180:234	Wild-type transthyretin-associated (ATTRwt) amyloidosis	180:234	Wild-type transthyretin-associated (ATTRwt) amyloidosis is an age-related disease that causes heart failure in older adults.					
33141553	9	59	theme	oligosaccharide	1701:1715	arg1	variations					1717:1726	CLU oligosaccharide variations	1697:1726	CLU oligosaccharide variations between patient and healthy controls	1697:1763	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	2	60	theme	fibril	354:359	arg1	deposits					361:368	cardiac amyloid fibril deposits	338:368	cardiac amyloid fibril deposits that originate from dissociation of the tetrameric protein, transthyretin (TTR)	338:448	This disease frequently features cardiac amyloid fibril deposits that originate from dissociation of the tetrameric protein, transthyretin (TTR).					
33141553	6	61	theme	amyloid	1129:1135	arg1	fibrils					1137:1143	amyloid fibrils	1129:1143	amyloid fibrils from ATTRwt cardiac tissue	1129:1170	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	9	62	theme	disease-specific	1870:1885	arg1	features					1898:1905	disease-specific structural features	1870:1905	disease-specific structural features that limit the chaperoning capacity of CLU	1870:1948	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	6	63	theme	serum	1265:1269	arg1	levels					1271:1276	(3) CLU serum levels	1257:1276	(3) CLU serum levels in patients with ATTRwt	1257:1300	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	2	64	theme	cardiac	338:344	arg1	deposits					361:368	cardiac amyloid fibril deposits	338:368	cardiac amyloid fibril deposits that originate from dissociation of the tetrameric protein, transthyretin (TTR)	338:448	This disease frequently features cardiac amyloid fibril deposits that originate from dissociation of the tetrameric protein, transthyretin (TTR).					
33141553	3	65	theme	protein	605:611	arg1	alterations					622:632	protein sequence alterations	605:632	protein sequence alterations	605:632	Unlike hereditary TTR (ATTRm) amyloidosis, where amino acid replacements destabilize the native protein, in ATTRwt amyloidosis, amyloid-forming TTR lacks protein sequence alterations.					
33141553	4	66	theme	formation	666:674	arg1	cause					650:654	The initiating cause	635:654	The initiating cause of fibril formation in ATTRwt amyloidosis	635:696	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	4	66	theme	formation	666:674	arg1	unclear					701:707	unclear	701:707	unclear	701:707	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	0	67	theme	Mass	152:155	arg1	Analyses					170:177	Mass Spectrometry Analyses	152:177	Mass Spectrometry Analyses	152:177	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	7	68	from	detail	1403:1408	arg1	analyses					1459:1466	mass spectrometry analyses	1441:1466	mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera	1441:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	4	69	theme	ATTRwt	679:684	arg1	amyloidosis					686:696	ATTRwt amyloidosis	679:696	ATTRwt amyloidosis	679:696	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	3	70	theme	sequence	613:620	arg1	alterations					622:632	protein sequence alterations	605:632	protein sequence alterations	605:632	Unlike hereditary TTR (ATTRm) amyloidosis, where amino acid replacements destabilize the native protein, in ATTRwt amyloidosis, amyloid-forming TTR lacks protein sequence alterations.					
33141553	0	71	theme	Features	137:144	arg1	Study					95:99	A Study	93:99	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.	0:178	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	8	72	attach	derived	1608:1614	arg1	patients					1621:1628	patients	1621:1628	patients with ATTRwt amyloidosis	1621:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	8	72	attach	derived	1608:1614	arg2	CLU					1604:1606	serum CLU	1598:1606	serum CLU derived from patients with ATTRwt amyloidosis	1598:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	9	73	theme	chaperoning	1922:1932	arg1	capacity					1934:1941	the chaperoning capacity	1918:1941	the chaperoning capacity of CLU	1918:1948	Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU.					
33141553	8	74	from	characterization	1551:1566	arg1	CLU					1604:1606	serum CLU	1598:1606	serum CLU derived from patients with ATTRwt amyloidosis	1598:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	4	75	theme	wild-type	821:829	arg1	fibrils					835:841	wild-type TTR fibrils	821:841	wild-type TTR fibrils	821:841	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	3	76	theme	amyloid-forming	579:593	arg1	TTR					595:597	amyloid-forming TTR	579:597	amyloid-forming TTR	579:597	Unlike hereditary TTR (ATTRm) amyloidosis, where amino acid replacements destabilize the native protein, in ATTRwt amyloidosis, amyloid-forming TTR lacks protein sequence alterations.					
33141553	4	77	theme	other	744:748	arg1	factors					750:756	other factors	744:756	other factors	744:756	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	6	78	with	levels	1271:1276	arg1	TTR					1122:1124	non-native TTR	1111:1124	non-native TTR in amyloid fibrils from ATTRwt cardiac tissue	1111:1170	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	7	79	theme	CLU	1504:1506	arg1	content					1493:1499	glycan content	1486:1499	glycan content of CLU purified from ATTRwt and control sera	1486:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	7	79	theme	CLU	1504:1506	arg1	acid					1477:1480	amino acid	1471:1480	amino acid	1471:1480	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	6	80	dep	CLU	1177:1179	arg1	2					1174:1174	2	1174:1174	2	1174:1174	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	4	81	from	cause	650:654	arg1	amyloidosis					686:696	ATTRwt amyloidosis	679:696	ATTRwt amyloidosis	679:696	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	6	82	with	patients	1281:1288	arg1	ATTRwt					1295:1300	ATTRwt	1295:1300	ATTRwt	1295:1300	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	5	83	theme	circulating	904:914	arg1	clusterin					860:868	clusterin	860:868	clusterin (CLU, UniProtKB P10909)	860:892	We believe that clusterin (CLU, UniProtKB P10909), a plasma circulating glycoprotein, plays a role in the pathobiology of ATTRwt amyloidosis.					
33141553	5	83	theme	circulating	904:914	arg1	glycoprotein					916:927	a plasma circulating glycoprotein	895:927	a plasma circulating glycoprotein	895:927	We believe that clusterin (CLU, UniProtKB P10909), a plasma circulating glycoprotein, plays a role in the pathobiology of ATTRwt amyloidosis.					
33141553	7	84	theme	content	1493:1499	arg1	analyses					1459:1466	mass spectrometry analyses	1441:1466	mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera	1441:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	0	85	theme	Compositional	123:135	arg1	Features					137:144	Disease-Associated Compositional Features	104:144	Disease-Associated Compositional Features Using Mass Spectrometry Analyses	104:177	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	5	86	theme	ATTRwt	966:971	arg1	amyloidosis					973:983	ATTRwt amyloidosis	966:983	ATTRwt amyloidosis	966:983	We believe that clusterin (CLU, UniProtKB P10909), a plasma circulating glycoprotein, plays a role in the pathobiology of ATTRwt amyloidosis.					
33141553	0	87	theme	Disease-Associated	104:121	arg1	Features					137:144	Disease-Associated Compositional Features	104:144	Disease-Associated Compositional Features Using Mass Spectrometry Analyses	104:177	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	6	88	from	levels	1271:1276	arg1	patients					1281:1288	patients	1281:1288	patients with ATTRwt	1281:1300	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	4	89	theme	unregulated	793:803	arg1	accumulation					805:816	unregulated accumulation	793:816	unregulated accumulation	793:816	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	6	90	from	tissue	1165:1170	arg1	TTR					1122:1124	non-native TTR	1111:1124	non-native TTR in amyloid fibrils from ATTRwt cardiac tissue	1111:1170	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	6	90	from	tissue	1165:1170	arg1	fibrils					1137:1143	amyloid fibrils	1129:1143	amyloid fibrils from ATTRwt cardiac tissue	1129:1170	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	2	91	theme	protein	421:427	arg1	dissociation					390:401	dissociation	390:401	dissociation of the tetrameric protein, transthyretin (TTR)	390:448	This disease frequently features cardiac amyloid fibril deposits that originate from dissociation of the tetrameric protein, transthyretin (TTR).					
33141553	0	92	theme	Clusterin	23:31	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.	0:178	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	0	93	theme	Wild-Type	36:44	arg1	Amyloidosis					80:90	Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis	36:90	Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis	36:90	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	7	94	theme	acid	1477:1480	arg1	analyses					1459:1466	mass spectrometry analyses	1441:1466	mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera	1441:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	0	95	gly	Glycosylation	0:12	arg1	Clusterin					23:31	Serum Clusterin	17:31	Serum Clusterin	17:31	Glycosylation of Serum Clusterin in Wild-Type Transthyretin-Associated (ATTRwt) Amyloidosis: A Study of Disease-Associated Compositional Features Using Mass Spectrometry Analyses.					
33141553	6	96	theme	ATTRwt	1034:1039	arg1	amyloidosis					1041:1051	ATTRwt amyloidosis	1034:1051	ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls	1034:1353	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	6	97	from	codeposits	1095:1104	arg1	patients					1281:1288	patients	1281:1288	patients with ATTRwt	1281:1300	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	6	98	theme	non-native	1111:1120	arg1	TTR					1122:1124	non-native TTR	1111:1124	non-native TTR in amyloid fibrils from ATTRwt cardiac tissue	1111:1170	Previously, we have suggested a role for CLU in ATTRwt amyloidosis based on our studies showing that (1) CLU codeposits with non-native TTR in amyloid fibrils from ATTRwt cardiac tissue, (2) CLU interacts only with non-native (monomeric and aggregated) forms of TTR, and (3) CLU serum levels in patients with ATTRwt are significantly lower compared to healthy controls.					
33141553	8	99	with	patients	1621:1628	arg1	amyloidosis					1642:1652	ATTRwt amyloidosis	1635:1652	ATTRwt amyloidosis	1635:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	7	100	theme	spectrometry	1446:1457	arg1	analyses					1459:1466	mass spectrometry analyses	1441:1466	mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera	1441:1544	In the present study, we provide comprehensive detail of compositional findings from mass spectrometry analyses of amino acid and glycan content of CLU purified from ATTRwt and control sera.					
33141553	8	101	from	content	1587:1593	arg1	CLU					1604:1606	serum CLU	1598:1606	serum CLU derived from patients with ATTRwt amyloidosis	1598:1652	The characterization of oligosaccharide content in serum CLU derived from patients with ATTRwt amyloidosis is novel data.					
33141553	3	102	theme	acid	506:509	arg1	replacements					511:522	amino acid replacements	500:522	amino acid replacements	500:522	Unlike hereditary TTR (ATTRm) amyloidosis, where amino acid replacements destabilize the native protein, in ATTRwt amyloidosis, amyloid-forming TTR lacks protein sequence alterations.					
33141553	4	103	theme	fibril	659:664	arg1	formation					666:674	fibril formation	659:674	fibril formation	659:674	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	4	104	theme	TTR	831:833	arg1	fibrils					835:841	wild-type TTR fibrils	821:841	wild-type TTR fibrils	821:841	The initiating cause of fibril formation in ATTRwt amyloidosis is unclear, and thus, it seems plausible that other factors are involved in TTR misfolding and unregulated accumulation of wild-type TTR fibrils.					
33141553	1	105	theme	heart	274:278	arg1	failure					280:286	heart failure	274:286	heart failure	274:286	Wild-type transthyretin-associated (ATTRwt) amyloidosis is an age-related disease that causes heart failure in older adults.					
33157282	7	0	theme	molecular	1156:1164	arg1	structures					1173:1182	high molecular weight structures	1151:1182	high molecular weight structures	1151:1182	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	3	1	theme	in	551:552	arg1	modification					559:570	the in vivo modification	547:570	the in vivo modification of N-glycosylation pattern in this tissue	547:612	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	9	2	theme	mammary	1693:1699	arg1	gland					1701:1705	the mammary gland	1689:1705	the mammary gland	1689:1705	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	3	3	theme	N-glycosylation	575:589	arg1	pattern					591:597	N-glycosylation pattern	575:597	N-glycosylation pattern	575:597	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	5	4	theme	mass	896:899	arg1	spectrometry					901:912	mass spectrometry	896:912	mass spectrometry	896:912	Protein detection was assessed by gel electrophoresis and western blot, and N-glycans were identified by HPLC and mass spectrometry.					
33157282	6	5	theme	GnT-IVa	915:921	arg1	overexpression					923:936	GnT-IVa overexpression	915:936	GnT-IVa overexpression	915:936	GnT-IVa overexpression and its colocalization with EPO-Fc in the Golgi apparatus of SiHa cells were demonstrated.					
33157282	2	6	theme	glycoproteins	344:356	arg1	branching					319:327	oligosaccharide branching	303:327	oligosaccharide branching of the desired glycoproteins	303:356	Overexpression of glycosyltransferases may increase oligosaccharide branching of the desired glycoproteins.					
33157282	1	7	theme	N-glycosylation	188:202	arg1	pattern					177:183	an aberrant pattern	165:183	an aberrant pattern of N-glycosylation	165:202	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	9	8	from	pattern	1590:1596	arg1	gland					1618:1622	the goat mammary gland	1601:1622	the goat mammary gland	1601:1622	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	8	9	theme	gland	1410:1414	arg1	pathway					1426:1432	the goat mammary gland secretion pathway	1393:1432	the goat mammary gland secretion pathway	1393:1432	The results confirmed that successful modification of the goat mammary gland secretion pathway could be achieved by co-expressing glycoenzymes together with the glycoprotein of interest.					
33157282	6	10	theme	SiHa	999:1002	arg1	cells					1004:1008	SiHa cells	999:1008	SiHa cells	999:1008	GnT-IVa overexpression and its colocalization with EPO-Fc in the Golgi apparatus of SiHa cells were demonstrated.					
33157282	7	11	from	N-glycans	1232:1240	arg1	tissue					1331:1336	transformed mammary tissue	1311:1336	transformed mammary tissue	1311:1336	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	11	from	N-glycans	1232:1240	arg1	milk					1301:1304	goat's milk	1294:1304	goat's milk from transformed mammary tissue	1294:1336	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	11	from	N-glycans	1232:1240	arg1	cells					1250:1254	SiHa cells	1245:1254	SiHa cells	1245:1254	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	4	12	theme	in	698:699	arg1	expression					719:728	in vitro and in vivo expression	698:728	in vitro and in vivo expression in mammalian cell culture or in goat mammary gland	698:779	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	9	13	theme	first	1538:1542	arg1	report					1544:1549	the first report	1534:1549	the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo	1534:1630	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	9	13	theme	first	1538:1542	arg1	This					1526:1529	This	1526:1529	This	1526:1529	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	9	14	theme	recombinant	1753:1763	arg1	proteins					1765:1772	complex recombinant proteins	1745:1772	complex recombinant proteins	1745:1772	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	8	15	theme	interest	1516:1523	arg1	glycoprotein					1500:1511	the glycoprotein	1496:1511	the glycoprotein of interest	1496:1523	The results confirmed that successful modification of the goat mammary gland secretion pathway could be achieved by co-expressing glycoenzymes together with the glycoprotein of interest.					
33157282	7	16	theme	EPO-Fc	1085:1090	arg1	expression					1071:1080	in vitro and in vivo expression	1050:1080	in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa)	1050:1127	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	17	theme	in	1050:1051	arg1	expression					1071:1080	in vitro and in vivo expression	1050:1080	in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa)	1050:1127	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	4	18	theme	goat	762:765	arg1	gland					775:779	goat mammary gland	762:779	goat mammary gland	762:779	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	7	19	theme	expression	1071:1080	arg1	analysis					1038:1045	N-glycan analysis	1029:1045	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa)	1029:1127	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	2	20	theme	glycosyltransferases	269:288	arg1	Overexpression					251:264	Overexpression	251:264	Overexpression of glycosyltransferases	251:288	Overexpression of glycosyltransferases may increase oligosaccharide branching of the desired glycoproteins.					
33157282	3	21	theme	human	365:369	arg1	erythropoietin					371:384	human erythropoietin	365:384	human erythropoietin fused to human IgG Fc (EPO-Fc)	365:415	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	7	22	theme	tri-antennary	1267:1279	arg1	N-glycans					1281:1289	mostly tri-antennary N-glycans	1260:1289	mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue	1260:1336	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	9	23	theme	N-glycosylation	1574:1588	arg1	pattern					1590:1596	the N-glycosylation pattern	1570:1596	the N-glycosylation pattern in the goat mammary gland in vivo	1570:1630	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	4	24	theme	cell	743:746	arg1	culture					748:754	mammalian cell culture	733:754	mammalian cell culture	733:754	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	3	25	theme	human	395:399	arg1	EPO-Fc					409:414	EPO-Fc	409:414	EPO-Fc	409:414	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	3	25	theme	human	395:399	arg1	Fc					405:406	human IgG Fc	395:406	human IgG Fc (EPO-Fc)	395:415	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	7	26	theme	SiHa	1245:1248	arg1	cells					1250:1254	SiHa cells	1245:1254	SiHa cells	1245:1254	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	9	27	theme	goat	1605:1608	arg1	gland					1618:1622	the goat mammary gland	1601:1622	the goat mammary gland	1601:1622	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	8	28	theme	mammary	1402:1408	arg1	pathway					1426:1432	the goat mammary gland secretion pathway	1393:1432	the goat mammary gland secretion pathway	1393:1432	The results confirmed that successful modification of the goat mammary gland secretion pathway could be achieved by co-expressing glycoenzymes together with the glycoprotein of interest.					
33157282	5	29	theme	Protein	782:788	arg1	detection					790:798	Protein detection	782:798	Protein detection	782:798	Protein detection was assessed by gel electrophoresis and western blot, and N-glycans were identified by HPLC and mass spectrometry.					
33157282	0	30	theme	goat	28:31	arg1	pathway					61:67	the goat mammary gland glycosylation pathway	24:67	the goat mammary gland glycosylation pathway	24:67	In vivo modification of the goat mammary gland glycosylation pathway.					
33157282	1	31	theme	Complex	70:76	arg1	glycoproteins					90:102	Complex recombinant glycoproteins	70:102	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk	70:158	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	8	32	theme	secretion	1416:1424	arg1	pathway					1426:1432	the goat mammary gland secretion pathway	1393:1432	the goat mammary gland secretion pathway	1393:1432	The results confirmed that successful modification of the goat mammary gland secretion pathway could be achieved by co-expressing glycoenzymes together with the glycoprotein of interest.					
33157282	7	33	dep	in	1063:1064	arg1	vivo					1066:1069	vivo	1066:1069	vivo	1066:1069	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	0	34	theme	In	0:1	arg1	modification					8:19	In vivo modification	0:19	In vivo modification of the goat mammary gland glycosylation pathway	0:67	In vivo modification of the goat mammary gland glycosylation pathway.					
33157282	3	35	dep	in	551:552	arg1	vivo					554:557	vivo	554:557	vivo	554:557	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	3	36	theme	adenoviral	489:498	arg1	transduction					500:511	adenoviral transduction	489:511	adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue	489:612	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	8	37	gly	glycoprotein	1500:1511	arg1	glycoprotein					1500:1511	the glycoprotein	1496:1511	the glycoprotein of interest	1496:1523	The results confirmed that successful modification of the goat mammary gland secretion pathway could be achieved by co-expressing glycoenzymes together with the glycoprotein of interest.					
33157282	7	38	from	tissue	1331:1336	arg1	N-glycans					1281:1289	mostly tri-antennary N-glycans	1260:1289	mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue	1260:1336	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	38	from	tissue	1331:1336	arg1	N-glycans					1232:1240	tri- and tetra-antennary N-glycans	1207:1240	tri- and tetra-antennary N-glycans in SiHa cells	1207:1254	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	38	from	tissue	1331:1336	arg1	milk					1301:1304	goat's milk	1294:1304	goat's milk from transformed mammary tissue	1294:1336	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	0	39	theme	glycosylation	47:59	arg1	pathway					61:67	the goat mammary gland glycosylation pathway	24:67	the goat mammary gland glycosylation pathway	24:67	In vivo modification of the goat mammary gland glycosylation pathway.					
33157282	3	40	from	transduction	500:511	arg1	gland					529:533	goat mammary gland	516:533	goat mammary gland	516:533	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	5	41	theme	western	840:846	arg1	blot					848:851	western blot	840:851	western blot	840:851	Protein detection was assessed by gel electrophoresis and western blot, and N-glycans were identified by HPLC and mass spectrometry.					
33157282	9	42	theme	forward	1656:1662	arg1	step					1651:1654	a step	1649:1654	a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins	1649:1772	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	6	43	with	colocalization	946:959	arg1	EPO-Fc					966:971	EPO-Fc	966:971	EPO-Fc	966:971	GnT-IVa overexpression and its colocalization with EPO-Fc in the Golgi apparatus of SiHa cells were demonstrated.					
33157282	3	44	theme	mammary	521:527	arg1	gland					529:533	goat mammary gland	516:533	goat mammary gland	516:533	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	4	45	dep	in	711:712	arg1	vivo					714:717	vivo	714:717	vivo	714:717	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	4	46	theme	EPO-Fc	650:655	arg1	sequences					669:677	the EPO-Fc and GnT-IVa sequences	646:677	the EPO-Fc and GnT-IVa sequences	646:677	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	2	47	gly	glycoproteins	344:356	arg1	glycoproteins					344:356	the desired glycoproteins	332:356	the desired glycoproteins	332:356	Overexpression of glycosyltransferases may increase oligosaccharide branching of the desired glycoproteins.					
33157282	1	48	theme	structures	239:248	arg1	lack					215:218	the lack	211:218	the lack of multi-antennary structures	211:248	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	0	49	theme	gland	41:45	arg1	pathway					61:67	the goat mammary gland glycosylation pathway	24:67	the goat mammary gland glycosylation pathway	24:67	In vivo modification of the goat mammary gland glycosylation pathway.					
33157282	0	50	dep	In	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	In vivo modification of the goat mammary gland glycosylation pathway.					
33157282	4	51	theme	GnT-IVa	661:667	arg1	sequences					669:677	the EPO-Fc and GnT-IVa sequences	646:677	the EPO-Fc and GnT-IVa sequences	646:677	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	7	52	theme	high	1151:1154	arg1	structures					1173:1182	high molecular weight structures	1151:1182	high molecular weight structures	1151:1182	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	53	from	N-glycans	1281:1289	arg1	tissue					1331:1336	transformed mammary tissue	1311:1336	transformed mammary tissue	1311:1336	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	53	from	N-glycans	1281:1289	arg1	milk					1301:1304	goat's milk	1294:1304	goat's milk from transformed mammary tissue	1294:1336	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	53	from	N-glycans	1281:1289	arg1	cells					1250:1254	SiHa cells	1245:1254	SiHa cells	1245:1254	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	6	54	from	overexpression	923:936	arg1	apparatus					986:994	the Golgi apparatus	976:994	the Golgi apparatus of SiHa cells	976:1008	GnT-IVa overexpression and its colocalization with EPO-Fc in the Golgi apparatus of SiHa cells were demonstrated.					
33157282	3	55	theme	pattern	591:597	arg1	modification					559:570	the in vivo modification	547:570	the in vivo modification of N-glycosylation pattern in this tissue	547:612	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	4	56	from	expression	719:728	arg1	gland					775:779	goat mammary gland	762:779	goat mammary gland	762:779	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	4	56	from	expression	719:728	arg1	culture					748:754	mammalian cell culture	733:754	mammalian cell culture	733:754	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	9	57	theme	gland	1701:1705	arg1	use					1682:1684	the use	1678:1684	the use of the mammary gland as a bioreactor for the production of complex recombinant proteins	1678:1772	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	1	58	contain	have	160:163	arg1	glycoproteins					90:102	Complex recombinant glycoproteins	70:102	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk	70:158	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	1	58	contain	have	160:163	arg2	pattern					177:183	an aberrant pattern	165:183	an aberrant pattern of N-glycosylation	165:202	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	3	59	from	modification	559:570	arg1	tissue					607:612	this tissue	602:612	this tissue	602:612	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	2	60	theme	oligosaccharide	303:317	arg1	branching					319:327	oligosaccharide branching	303:327	oligosaccharide branching of the desired glycoproteins	303:356	Overexpression of glycosyltransferases may increase oligosaccharide branching of the desired glycoproteins.					
33157282	1	61	theme	aberrant	168:175	arg1	pattern					177:183	an aberrant pattern	165:183	an aberrant pattern of N-glycosylation	165:202	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	0	62	theme	mammary	33:39	arg1	pathway					61:67	the goat mammary gland glycosylation pathway	24:67	the goat mammary gland glycosylation pathway	24:67	In vivo modification of the goat mammary gland glycosylation pathway.					
33157282	9	63	from	modification	1554:1565	arg1	gland					1618:1622	the goat mammary gland	1601:1622	the goat mammary gland	1601:1622	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	4	64	theme	in	711:712	arg1	expression					719:728	in vitro and in vivo expression	698:728	in vitro and in vivo expression in mammalian cell culture or in goat mammary gland	698:779	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	2	65	theme	desired	336:342	arg1	glycoproteins					344:356	the desired glycoproteins	332:356	the desired glycoproteins	332:356	Overexpression of glycosyltransferases may increase oligosaccharide branching of the desired glycoproteins.					
33157282	6	66	theme	cells	1004:1008	arg1	apparatus					986:994	the Golgi apparatus	976:994	the Golgi apparatus of SiHa cells	976:1008	GnT-IVa overexpression and its colocalization with EPO-Fc in the Golgi apparatus of SiHa cells were demonstrated.					
33157282	4	67	theme	mammalian	733:741	arg1	culture					748:754	mammalian cell culture	733:754	mammalian cell culture	733:754	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	7	68	theme	mammary	1323:1329	arg1	tissue					1331:1336	transformed mammary tissue	1311:1336	transformed mammary tissue	1311:1336	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	4	69	contain	containing	635:644	arg2	sequences					669:677	the EPO-Fc and GnT-IVa sequences	646:677	the EPO-Fc and GnT-IVa sequences	646:677	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	4	69	contain	containing	635:644	arg1	vectors					626:632	Adenoviral vectors	615:632	Adenoviral vectors	615:632	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	9	70	theme	complex	1745:1751	arg1	proteins					1765:1772	complex recombinant proteins	1745:1772	complex recombinant proteins	1745:1772	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	7	71	theme	in	1063:1064	arg1	expression					1071:1080	in vitro and in vivo expression	1050:1080	in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa)	1050:1127	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	1	72	theme	potential	116:124	arg1	biopharmaceuticals					126:143	potential biopharmaceuticals	116:143	potential biopharmaceuticals in goat's milk	116:158	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	6	73	theme	Golgi	980:984	arg1	apparatus					986:994	the Golgi apparatus	976:994	the Golgi apparatus of SiHa cells	976:1008	GnT-IVa overexpression and its colocalization with EPO-Fc in the Golgi apparatus of SiHa cells were demonstrated.					
33157282	9	74	theme	proteins	1765:1772	arg1	production					1731:1740	the production	1727:1740	the production of complex recombinant proteins	1727:1772	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	9	75	theme	modification	1554:1565	arg1	report					1544:1549	the first report	1534:1549	the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo	1534:1630	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	9	75	theme	modification	1554:1565	arg1	This					1526:1529	This	1526:1529	This	1526:1529	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	0	76	theme	pathway	61:67	arg1	modification					8:19	In vivo modification	0:19	In vivo modification of the goat mammary gland glycosylation pathway	0:67	In vivo modification of the goat mammary gland glycosylation pathway.					
33157282	7	77	theme	N-glycan	1029:1036	arg1	analysis					1038:1045	N-glycan analysis	1029:1045	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa)	1029:1127	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	9	78	theme	pattern	1590:1596	arg1	modification					1554:1565	modification	1554:1565	modification of the N-glycosylation pattern in the goat mammary gland in vivo	1554:1630	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	9	79	from	gland	1618:1622	arg1	modification					1554:1565	modification	1554:1565	modification of the N-glycosylation pattern in the goat mammary gland in vivo	1554:1630	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	8	80	theme	successful	1366:1375	arg1	modification					1377:1388	successful modification	1366:1388	successful modification of the goat mammary gland secretion pathway	1366:1432	The results confirmed that successful modification of the goat mammary gland secretion pathway could be achieved by co-expressing glycoenzymes together with the glycoprotein of interest.					
33157282	1	81	gly	glycoproteins	90:102	arg1	glycoproteins					90:102	Complex recombinant glycoproteins	70:102	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk	70:158	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	3	82	theme	IgG	401:403	arg1	EPO-Fc					409:414	EPO-Fc	409:414	EPO-Fc	409:414	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	3	82	theme	IgG	401:403	arg1	Fc					405:406	human IgG Fc	395:406	human IgG Fc (EPO-Fc)	395:415	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	7	83	dep	in	1050:1051	arg1	vitro					1053:1057	vitro	1053:1057	vitro	1053:1057	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	84	theme	tetra-antennary	1216:1230	arg1	N-glycans					1232:1240	tri- and tetra-antennary N-glycans	1207:1240	tri- and tetra-antennary N-glycans in SiHa cells	1207:1254	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	9	85	theme	mammary	1610:1616	arg1	gland					1618:1622	the goat mammary gland	1601:1622	the goat mammary gland	1601:1622	This is the first report of modification of the N-glycosylation pattern in the goat mammary gland in vivo, and constitutes a step forward for improving the use of the mammary gland as a bioreactor for the production of complex recombinant proteins.					
33157282	8	86	theme	goat	1397:1400	arg1	pathway					1426:1432	the goat mammary gland secretion pathway	1393:1432	the goat mammary gland secretion pathway	1393:1432	The results confirmed that successful modification of the goat mammary gland secretion pathway could be achieved by co-expressing glycoenzymes together with the glycoprotein of interest.					
33157282	7	87	theme	tri-	1207:1210	arg1	N-glycans					1232:1240	tri- and tetra-antennary N-glycans	1207:1240	tri- and tetra-antennary N-glycans in SiHa cells	1207:1254	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	1	88	theme	recombinant	78:88	arg1	glycoproteins					90:102	Complex recombinant glycoproteins	70:102	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk	70:158	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	4	89	theme	mammary	767:773	arg1	gland					775:779	goat mammary gland	762:779	goat mammary gland	762:779	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	1	90	from	biopharmaceuticals	126:143	arg1	milk					155:158	goat's milk	148:158	goat's milk	148:158	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	4	91	theme	Adenoviral	615:624	arg1	vectors					626:632	Adenoviral vectors	615:632	Adenoviral vectors	615:632	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	8	92	theme	pathway	1426:1432	arg1	modification					1377:1388	successful modification	1366:1388	successful modification of the goat mammary gland secretion pathway	1366:1432	The results confirmed that successful modification of the goat mammary gland secretion pathway could be achieved by co-expressing glycoenzymes together with the glycoprotein of interest.					
33157282	3	93	theme	goat	516:519	arg1	gland					529:533	goat mammary gland	516:533	goat mammary gland	516:533	Here, human erythropoietin fused to human IgG Fc (EPO-Fc) was co-expressed with N-acetyl-glucosaminyltransferase-IVa (GnT-IVa) by adenoviral transduction in goat mammary gland to evaluate the in vivo modification of N-glycosylation pattern in this tissue.					
33157282	5	94	theme	gel	816:818	arg1	electrophoresis					820:834	gel electrophoresis	816:834	gel electrophoresis	816:834	Protein detection was assessed by gel electrophoresis and western blot, and N-glycans were identified by HPLC and mass spectrometry.					
33157282	4	95	dep	in	698:699	arg1	vitro					701:705	vitro	701:705	vitro	701:705	Adenoviral vectors, containing the EPO-Fc and GnT-IVa sequences were assembled for in vitro and in vivo expression in mammalian cell culture or in goat mammary gland.					
33157282	7	96	from	increase	1139:1146	arg1	structures					1173:1182	high molecular weight structures	1151:1182	high molecular weight structures	1151:1182	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	97	theme	weight	1166:1171	arg1	structures					1173:1182	high molecular weight structures	1151:1182	high molecular weight structures	1151:1182	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	7	98	theme	transformed	1311:1321	arg1	tissue					1331:1336	transformed mammary tissue	1311:1336	transformed mammary tissue	1311:1336	N-glycan analysis of in vitro and in vivo expression of EPO-Fc modified by GnT-IVa (EPO-Fc/GnT-IVa) showed an increase in high molecular weight structures, which corresponded to tri- and tetra-antennary N-glycans in SiHa cells and mostly tri-antennary N-glycans in goat's milk from transformed mammary tissue.					
33157282	1	99	theme	multi-antennary	223:237	arg1	structures					239:248	multi-antennary structures	223:248	multi-antennary structures	223:248	Complex recombinant glycoproteins produced as potential biopharmaceuticals in goat's milk have an aberrant pattern of N-glycosylation due to the lack of multi-antennary structures.					
33157282	6	100	with	overexpression	923:936	arg1	EPO-Fc					966:971	EPO-Fc	966:971	EPO-Fc	966:971	GnT-IVa overexpression and its colocalization with EPO-Fc in the Golgi apparatus of SiHa cells were demonstrated.					
33157282	6	101	from	colocalization	946:959	arg1	apparatus					986:994	the Golgi apparatus	976:994	the Golgi apparatus of SiHa cells	976:1008	GnT-IVa overexpression and its colocalization with EPO-Fc in the Golgi apparatus of SiHa cells were demonstrated.					
34318818	0	0	theme	glycosylation	73:85	arg1	states					12:17	Protonation states	0:17	Protonation states at different pH	0:33	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	0	0	theme	glycosylation	73:85	arg1	impact					63:68	impact	63:68	impact of glycosylation in synapsin Ia	63:100	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	0	0	theme	glycosylation	73:85	arg1	changes					51:57	conformational changes	36:57	conformational changes	36:57	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	2	1	dep	basic	543:547	arg1	to					540:541	to	540:541	to	540:541	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	8	2	theme	glycosylated	1438:1449	arg1	protein					1470:1476	the glycosylated and unglycosylated protein	1434:1476	the glycosylated and unglycosylated protein	1434:1476	Moreover, the results obtained by comparing the glycosylated and unglycosylated protein indicated that the glycan moiety imparts stability to the protein by forming intramolecular hydrogen bond interactions with the protein residues.					
34318818	8	3	gly	glycosylated	1438:1449	arg1	protein					1470:1476	the glycosylated and unglycosylated protein	1434:1476	the glycosylated and unglycosylated protein	1434:1476	Moreover, the results obtained by comparing the glycosylated and unglycosylated protein indicated that the glycan moiety imparts stability to the protein by forming intramolecular hydrogen bond interactions with the protein residues.					
34318818	8	4	theme	protein	1606:1612	arg1	residues					1614:1621	the protein residues	1602:1621	the protein residues	1602:1621	Moreover, the results obtained by comparing the glycosylated and unglycosylated protein indicated that the glycan moiety imparts stability to the protein by forming intramolecular hydrogen bond interactions with the protein residues.					
34318818	5	5	theme	simulation	1111:1120	arg1	approach					1122:1129	molecular dynamics (MD) simulation approach	1087:1129	molecular dynamics (MD) simulation approach	1087:1129	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	4	6	theme	possible	914:921	arg1	link					923:926	a possible link	912:926	a possible link to deficits in synaptic plasticity	912:961	Furthermore, reduced levels of O-GlcNAc have been observed in Alzheimer's disease, suggesting a possible link to deficits in synaptic plasticity.					
34318818	3	7	theme	SynIa	644:648	arg1	aspect					634:639	Another important aspect	616:639	Another important aspect of SynIa	616:648	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	2	8	theme	conformational	556:569	arg1	stability					571:579	the conformational stability	552:579	the conformational stability of SynI	552:587	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	6	9	theme	homology	1194:1201	arg1	methods					1212:1218	threading-based homology modeling methods	1178:1218	threading-based homology modeling methods	1178:1218	The 3D structure of SynIa was established via threading-based homology modeling methods.					
34318818	0	10	theme	synapsin	90:97	arg1	Ia					99:100	synapsin Ia	90:100	synapsin Ia	90:100	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	1	11	theme	neurite	326:332	arg1	outgrowth					334:342	neurite outgrowth	326:342	neurite outgrowth	326:342	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	6	12	theme	modeling	1203:1210	arg1	methods					1212:1218	threading-based homology modeling methods	1178:1218	threading-based homology modeling methods	1178:1218	The 3D structure of SynIa was established via threading-based homology modeling methods.					
34318818	8	13	gly	unglycosylated	1455:1468	arg1	protein					1470:1476	the glycosylated and unglycosylated protein	1434:1476	the glycosylated and unglycosylated protein	1434:1476	Moreover, the results obtained by comparing the glycosylated and unglycosylated protein indicated that the glycan moiety imparts stability to the protein by forming intramolecular hydrogen bond interactions with the protein residues.					
34318818	1	14	theme	synaptic	251:258	arg1	SVs					270:272	SVs	270:272	SVs	270:272	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	14	theme	synaptic	251:258	arg1	vesicles					260:267	synaptic vesicles	251:267	synaptic vesicles (SVs) at active zones	251:289	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	9	15	theme	SynIa	1715:1719	arg1	structure					1721:1729	SynIa structure	1715:1729	SynIa structure	1715:1729	The results indicate that although O-GlcNAc moieties do not induce a significant change in SynIa structure they minimize protein dynamics, likely leading to enhanced protein stability.					
34318818	0	16	from	changes	51:57	arg1	Ia					99:100	synapsin Ia	90:100	synapsin Ia	90:100	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	1	17	from	zones	285:289	arg1	release					228:234	neurotransmitter release	211:234	neurotransmitter release	211:234	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	17	from	zones	285:289	arg1	SVs					270:272	SVs	270:272	SVs	270:272	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	17	from	zones	285:289	arg1	clustering					237:246	clustering	237:246	clustering of synaptic vesicles (SVs) at active zones	237:289	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	17	from	zones	285:289	arg1	vesicles					260:267	synaptic vesicles	251:267	synaptic vesicles (SVs) at active zones	251:289	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	3	18	attach	linked	779:784	arg1	memory					802:807	memory	802:807	memory	802:807	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	3	18	attach	linked	779:784	arg2	plasticity					768:777	synaptic plasticity	759:777	synaptic plasticity linked to learning and memory in mice	759:815	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	3	18	attach	linked	779:784	arg1	learning					789:796	learning	789:796	learning	789:796	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	1	19	theme	vesicles	260:267	arg1	release					228:234	neurotransmitter release	211:234	neurotransmitter release	211:234	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	19	theme	vesicles	260:267	arg1	clustering					237:246	clustering	237:246	clustering of synaptic vesicles (SVs) at active zones	237:289	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	6	20	theme	threading-based	1178:1192	arg1	methods					1212:1218	threading-based homology modeling methods	1178:1218	threading-based homology modeling methods	1178:1218	The 3D structure of SynIa was established via threading-based homology modeling methods.					
34318818	9	21	theme	protein	1745:1751	arg1	dynamics					1753:1760	protein dynamics	1745:1760	protein dynamics	1745:1760	The results indicate that although O-GlcNAc moieties do not induce a significant change in SynIa structure they minimize protein dynamics, likely leading to enhanced protein stability.					
34318818	1	22	theme	abundant	133:140	arg1	phosphoprotein					148:161	the most abundant brain phosphoprotein	124:161	the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth	124:342	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	22	theme	abundant	133:140	arg1	Synapsin					103:110	Synapsin I	103:112	Synapsin I (SynI)	103:119	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	3	23	theme	plasticity	768:777	arg1	regulation					745:754	the positive regulation	732:754	the positive regulation of synaptic plasticity linked to learning and memory in mice	732:815	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	7	24	theme	compact	1358:1364	arg1	conformation					1366:1377	a compact conformation	1356:1377	a compact conformation at pH 8.0	1356:1387	It was observed that the structure of SynIa adopts extended conformational changes as the pH shifts from acidic to basic, resulting in a compact conformation at pH 8.0.					
34318818	3	25	from	position	697:704	arg1	O-GlcNac					674:681	O-GlcNac	674:681	O-GlcNac	674:681	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	3	25	from	position	697:704	arg1	O-GlcNAcylation					657:671	its O-GlcNAcylation	653:671	its O-GlcNAcylation (O-GlcNac)	653:682	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	2	26	theme	actin	430:434	arg1	filaments					436:444	actin filaments	430:444	actin filaments	430:444	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	1	27	theme	brain	142:146	arg1	phosphoprotein					148:161	the most abundant brain phosphoprotein	124:161	the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth	124:342	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	27	theme	brain	142:146	arg1	Synapsin					103:110	Synapsin I	103:112	Synapsin I (SynI)	103:119	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	0	28	from	pH	32:33	arg1	impact					63:68	impact	63:68	impact of glycosylation in synapsin Ia	63:100	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	0	28	from	pH	32:33	arg1	states					12:17	Protonation states	0:17	Protonation states at different pH	0:33	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	0	28	from	pH	32:33	arg1	changes					51:57	conformational changes	36:57	conformational changes	36:57	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	2	29	theme	SynI	584:587	arg1	stability					571:579	the conformational stability	552:579	the conformational stability of SynI	552:587	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	3	30	from	learning	789:796	arg1	mice					812:815	mice	812:815	mice	812:815	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	0	31	theme	Protonation	0:10	arg1	impact					63:68	impact	63:68	impact of glycosylation in synapsin Ia	63:100	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	0	31	theme	Protonation	0:10	arg1	states					12:17	Protonation states	0:17	Protonation states at different pH	0:33	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	0	31	theme	Protonation	0:10	arg1	changes					51:57	conformational changes	36:57	conformational changes	36:57	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	5	32	theme	dynamics	1097:1104	arg1	approach					1122:1129	molecular dynamics (MD) simulation approach	1087:1129	molecular dynamics (MD) simulation approach	1087:1129	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	1	33	theme	present	163:169	arg1	phosphoprotein					148:161	the most abundant brain phosphoprotein	124:161	the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth	124:342	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	33	theme	present	163:169	arg1	Synapsin					103:110	Synapsin I	103:112	Synapsin I (SynI)	103:119	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	0	34	theme	different	22:30	arg1	pH					32:33	different pH	22:33	different pH	22:33	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	8	35	theme	unglycosylated	1455:1468	arg1	protein					1470:1476	the glycosylated and unglycosylated protein	1434:1476	the glycosylated and unglycosylated protein	1434:1476	Moreover, the results obtained by comparing the glycosylated and unglycosylated protein indicated that the glycan moiety imparts stability to the protein by forming intramolecular hydrogen bond interactions with the protein residues.					
34318818	2	36	theme	shift	522:526	arg1	effect					509:514	the effect	505:514	the effect of pH shift from acidic to basic on the conformational stability of SynI	505:587	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	7	37	theme	extended	1272:1279	arg1	changes					1296:1302	extended conformational changes	1272:1302	extended conformational changes	1272:1302	It was observed that the structure of SynIa adopts extended conformational changes as the pH shifts from acidic to basic, resulting in a compact conformation at pH 8.0.					
34318818	3	38	from	memory	802:807	arg1	mice					812:815	mice	812:815	mice	812:815	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	3	39	theme	synaptic	759:766	arg1	plasticity					768:777	synaptic plasticity	759:777	synaptic plasticity linked to learning and memory in mice	759:815	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	9	40	theme	enhanced	1781:1788	arg1	stability					1798:1806	enhanced protein stability	1781:1806	enhanced protein stability	1781:1806	The results indicate that although O-GlcNAc moieties do not induce a significant change in SynIa structure they minimize protein dynamics, likely leading to enhanced protein stability.					
34318818	2	41	theme	SVs	423:425	arg1	tethering					410:418	pH-dependent tethering	397:418	pH-dependent tethering of SVs to actin filaments	397:444	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	2	42	theme	pH	519:520	arg1	shift					522:526	pH shift	519:526	pH shift from acidic to basic	519:547	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	7	43	from	pH	1382:1383	arg1	conformation					1366:1377	a compact conformation	1356:1377	a compact conformation at pH 8.0	1356:1387	It was observed that the structure of SynIa adopts extended conformational changes as the pH shifts from acidic to basic, resulting in a compact conformation at pH 8.0.					
34318818	1	44	theme	presynaptic	174:184	arg1	terminals					186:194	presynaptic terminals	174:194	presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth	174:342	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	3	45	theme	Thr87	691:695	arg1	position					697:704	the Thr87 position	687:704	the Thr87 position	687:704	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	1	46	from	clustering	237:246	arg1	zones					285:289	active zones	278:289	active zones	278:289	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	5	47	theme	pH	993:994	arg1	effect					983:988	the effect	979:988	the effect of pH and glycosylation on the structure and functional stability of SynIa	979:1063	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	6	48	theme	SynIa	1152:1156	arg1	structure					1139:1147	The 3D structure	1132:1147	The 3D structure of SynIa	1132:1156	The 3D structure of SynIa was established via threading-based homology modeling methods.					
34318818	2	49	theme	pH-dependent	397:408	arg1	tethering					410:418	pH-dependent tethering	397:418	pH-dependent tethering of SVs to actin filaments	397:444	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	1	50	theme	active	278:283	arg1	zones					285:289	active zones	278:289	active zones	278:289	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	51	attach	present	163:169	arg2	Synapsin					103:110	Synapsin I	103:112	Synapsin I (SynI)	103:119	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	51	attach	present	163:169	arg1	terminals					186:194	presynaptic terminals	174:194	presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth	174:342	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	51	attach	present	163:169	arg2	phosphoprotein					148:161	the most abundant brain phosphoprotein	124:161	the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth	124:342	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	52	from	terminals	186:194	arg1	present					163:169	present	163:169	present	163:169	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	4	53	theme	synaptic	943:950	arg1	plasticity					952:961	synaptic plasticity	943:961	synaptic plasticity	943:961	Furthermore, reduced levels of O-GlcNAc have been observed in Alzheimer's disease, suggesting a possible link to deficits in synaptic plasticity.					
34318818	5	54	dep	structure	1021:1029	arg1	the					1017:1019	the	1017:1019	the	1017:1019	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	9	55	theme	O-GlcNAc	1659:1666	arg1	moieties					1668:1675	O-GlcNAc moieties	1659:1675	O-GlcNAc moieties	1659:1675	The results indicate that although O-GlcNAc moieties do not induce a significant change in SynIa structure they minimize protein dynamics, likely leading to enhanced protein stability.					
34318818	5	56	theme	glycosylation	1000:1012	arg1	effect					983:988	the effect	979:988	the effect of pH and glycosylation on the structure and functional stability of SynIa	979:1063	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	8	57	theme	hydrogen	1570:1577	arg1	interactions					1584:1595	intramolecular hydrogen bond interactions	1555:1595	intramolecular hydrogen bond interactions	1555:1595	Moreover, the results obtained by comparing the glycosylated and unglycosylated protein indicated that the glycan moiety imparts stability to the protein by forming intramolecular hydrogen bond interactions with the protein residues.					
34318818	0	58	theme	conformational	36:49	arg1	states					12:17	Protonation states	0:17	Protonation states at different pH	0:33	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	0	58	theme	conformational	36:49	arg1	changes					51:57	conformational changes	36:57	conformational changes	36:57	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	4	59	from	deficits	931:938	arg1	plasticity					952:961	synaptic plasticity	943:961	synaptic plasticity	943:961	Furthermore, reduced levels of O-GlcNAc have been observed in Alzheimer's disease, suggesting a possible link to deficits in synaptic plasticity.					
34318818	4	60	theme	reduced	831:837	arg1	levels					839:844	reduced levels	831:844	reduced levels of O-GlcNAc	831:856	Furthermore, reduced levels of O-GlcNAc have been observed in Alzheimer's disease, suggesting a possible link to deficits in synaptic plasticity.					
34318818	6	61	theme	3D	1136:1137	arg1	structure					1139:1147	The 3D structure	1132:1147	The 3D structure of SynIa	1132:1156	The 3D structure of SynIa was established via threading-based homology modeling methods.					
34318818	0	62	from	impact	63:68	arg1	Ia					99:100	synapsin Ia	90:100	synapsin Ia	90:100	Protonation states at different pH, conformational changes and impact of glycosylation in synapsin Ia.					
34318818	9	63	theme	protein	1790:1796	arg1	stability					1798:1806	enhanced protein stability	1781:1806	enhanced protein stability	1781:1806	The results indicate that although O-GlcNAc moieties do not induce a significant change in SynIa structure they minimize protein dynamics, likely leading to enhanced protein stability.					
34318818	5	64	theme	molecular	1087:1095	arg1	MD					1107:1108	MD	1107:1108	MD	1107:1108	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	5	64	theme	molecular	1087:1095	arg1	dynamics					1097:1104	molecular dynamics	1087:1104	molecular dynamics (MD) simulation approach	1087:1129	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	4	65	theme	O-GlcNAc	849:856	arg1	levels					839:844	reduced levels	831:844	reduced levels of O-GlcNAc	831:856	Furthermore, reduced levels of O-GlcNAc have been observed in Alzheimer's disease, suggesting a possible link to deficits in synaptic plasticity.					
34318818	7	66	theme	conformational	1281:1294	arg1	changes					1296:1302	extended conformational changes	1272:1302	extended conformational changes	1272:1302	It was observed that the structure of SynIa adopts extended conformational changes as the pH shifts from acidic to basic, resulting in a compact conformation at pH 8.0.					
34318818	5	67	from	effect	983:988	arg1	structure					1021:1029	structure	1021:1029	structure	1021:1029	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	5	67	from	effect	983:988	arg1	stability					1046:1054	functional stability	1035:1054	functional stability	1035:1054	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	8	68	theme	intramolecular	1555:1568	arg1	interactions					1584:1595	intramolecular hydrogen bond interactions	1555:1595	intramolecular hydrogen bond interactions	1555:1595	Moreover, the results obtained by comparing the glycosylated and unglycosylated protein indicated that the glycan moiety imparts stability to the protein by forming intramolecular hydrogen bond interactions with the protein residues.					
34318818	8	69	theme	bond	1579:1582	arg1	interactions					1584:1595	intramolecular hydrogen bond interactions	1555:1595	intramolecular hydrogen bond interactions	1555:1595	Moreover, the results obtained by comparing the glycosylated and unglycosylated protein indicated that the glycan moiety imparts stability to the protein by forming intramolecular hydrogen bond interactions with the protein residues.					
34318818	9	70	theme	significant	1693:1703	arg1	change					1705:1710	a significant change	1691:1710	a significant change	1691:1710	The results indicate that although O-GlcNAc moieties do not induce a significant change in SynIa structure they minimize protein dynamics, likely leading to enhanced protein stability.					
34318818	7	71	dep	acidic	1326:1331	arg1	to					1333:1334	to	1333:1334	to	1333:1334	It was observed that the structure of SynIa adopts extended conformational changes as the pH shifts from acidic to basic, resulting in a compact conformation at pH 8.0.					
34318818	7	71	dep	acidic	1326:1331	arg1	basic					1336:1340	basic	1336:1340	basic	1336:1340	It was observed that the structure of SynIa adopts extended conformational changes as the pH shifts from acidic to basic, resulting in a compact conformation at pH 8.0.					
34318818	5	72	gly	glycosylation	1000:1012	arg1	SynIa					1059:1063	SynIa	1059:1063	SynIa	1059:1063	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	1	73	theme	neurotransmitter	211:226	arg1	release					228:234	neurotransmitter release	211:234	neurotransmitter release	211:234	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	1	73	theme	neurotransmitter	211:226	arg1	clustering					237:246	clustering	237:246	clustering of synaptic vesicles (SVs) at active zones	237:289	Synapsin I (SynI) is the most abundant brain phosphoprotein present at presynaptic terminals that regulates neurotransmitter release, clustering of synaptic vesicles (SVs) at active zones, and stimulates synaptogenesis and neurite outgrowth.					
34318818	3	74	theme	important	624:632	arg1	aspect					634:639	Another important aspect	616:639	Another important aspect of SynIa	616:648	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
34318818	5	75	theme	SynIa	1059:1063	arg1	structure					1021:1029	structure	1021:1029	structure	1021:1029	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	5	75	theme	SynIa	1059:1063	arg1	stability					1046:1054	functional stability	1035:1054	functional stability	1035:1054	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	7	76	theme	SynIa	1259:1263	arg1	structure					1246:1254	the structure	1242:1254	the structure of SynIa	1242:1263	It was observed that the structure of SynIa adopts extended conformational changes as the pH shifts from acidic to basic, resulting in a compact conformation at pH 8.0.					
34318818	8	77	theme	glycan	1497:1502	arg1	moiety					1504:1509	the glycan moiety	1493:1509	the glycan moiety	1493:1509	Moreover, the results obtained by comparing the glycosylated and unglycosylated protein indicated that the glycan moiety imparts stability to the protein by forming intramolecular hydrogen bond interactions with the protein residues.					
34318818	2	78	theme	neutral	484:490	arg1	pH					492:493	neutral pH	484:493	neutral pH	484:493	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	5	79	theme	functional	1035:1044	arg1	stability					1046:1054	functional stability	1035:1054	functional stability	1035:1054	In this study, the effect of pH and glycosylation on the structure and functional stability of SynIa is determined through molecular dynamics (MD) simulation approach.					
34318818	2	80	from	effect	509:514	arg1	stability					571:579	the conformational stability	552:579	the conformational stability of SynI	552:587	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	2	80	from	effect	509:514	arg1	basic					543:547	basic	543:547	basic	543:547	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	2	81	theme	Earlier	345:351	arg1	studies					353:359	Earlier studies	345:359	Earlier studies	345:359	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	2	82	from	basic	543:547	arg1	effect					509:514	the effect	505:514	the effect of pH shift from acidic to basic on the conformational stability of SynI	505:587	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	2	82	from	basic	543:547	arg1	shift					522:526	pH shift	519:526	pH shift from acidic to basic	519:547	Earlier studies have established that SynI displays pH-dependent tethering of SVs to actin filaments and exhibits a maximum binding around neutral pH, however, the effect of pH shift from acidic to basic on the conformational stability of SynI has not been explored yet.					
34318818	3	83	theme	positive	736:743	arg1	regulation					745:754	the positive regulation	732:754	the positive regulation of synaptic plasticity linked to learning and memory in mice	732:815	Another important aspect of SynIa is its O-GlcNAcylation (O-GlcNac) at the Thr87 position, which is responsible for the positive regulation of synaptic plasticity linked to learning and memory in mice.					
33510212	4	0	theme	pentasaccharides	1086:1101	arg1	pentasaccharides					1086:1101	two favored pentasaccharides	1074:1101	two favored pentasaccharides	1074:1101	Six mutants were selected for further characterization as they produce higher amounts of two favored pentasaccharides compared to the parental enzyme and/or new products.					
33510212	4	0	theme	pentasaccharides	1086:1101	arg1	amounts					1063:1069	higher amounts	1056:1069	higher amounts of two favored pentasaccharides	1056:1101	Six mutants were selected for further characterization as they produce higher amounts of two favored pentasaccharides compared to the parental enzyme and/or new products.					
33510212	3	1	from	family	712:717	arg1	enzyme					676:681	an enzyme	673:681	an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site	673:783	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	1	from	family	712:717	arg1	sucrase					664:670	ΔN123-GBD-CD2 branching sucrase	640:670	ΔN123-GBD-CD2 branching sucrase	640:670	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	2	2	theme	enzymes	377:383	arg1	Engineering					342:352	Engineering	342:352	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site,	342:432	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	5	3	theme	representative	1246:1259	arg1	haptens					1261:1267	representative haptens	1246:1267	representative haptens of Shigella flexneri serotypes 3a, 4a and 4b	1246:1312	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	9	4	theme	carbohydrate	1957:1968	arg1	components					1970:1979	carbohydrate components	1957:1979	carbohydrate components of bacterial haptens	1957:2000	They also provide new insight to further engineer this class of enzymes for the synthesis of carbohydrate components of bacterial haptens.					
33510212	9	5	theme	haptens	1994:2000	arg1	components					1970:1979	carbohydrate components	1957:1979	carbohydrate components of bacterial haptens	1957:2000	They also provide new insight to further engineer this class of enzymes for the synthesis of carbohydrate components of bacterial haptens.					
33510212	2	6	from	enzymes	377:383	arg1	particular					389:398	particular	389:398	particular	389:398	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	3	7	theme	active	773:778	arg1	site					780:783	the active site	769:783	the active site	769:783	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	8	8	theme	enzyme	1805:1810	arg1	structure					1812:1820	the enzyme structure	1801:1820	the enzyme structure	1801:1820	Our findings help to understand the inter-relationships between the enzyme structure, conformational flexibility and activity.					
33510212	1	9	theme	chemo-	186:191	arg1	synthesis					203:211	The (chemo-)enzymatic synthesis	181:211	The (chemo-)enzymatic synthesis of oligosaccharides	181:231	The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities.					
33510212	7	10	theme	amino	1708:1712	arg1	networks					1727:1734	amino acid residue networks	1708:1734	amino acid residue networks	1708:1734	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	2	11	theme	carbohydrate	580:591	arg1	synthesis					593:601	carbohydrate synthesis	580:601	carbohydrate synthesis	580:601	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	0	12	theme	S.	146:147	arg1	3a					158:159	S. flexneri 3a	146:159	S. flexneri 3a	146:159	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	1	13	theme	enzymatic	193:201	arg1	synthesis					203:211	The (chemo-)enzymatic synthesis	181:211	The (chemo-)enzymatic synthesis of oligosaccharides	181:231	The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities.					
33510212	2	14	theme	enzymes	568:574	arg1	enzymes					568:574	enzymes	568:574	enzymes for carbohydrate synthesis	568:601	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	2	14	theme	enzymes	568:574	arg1	repertoire					554:563	the repertoire	550:563	the repertoire of enzymes for carbohydrate synthesis	550:601	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	0	15	theme	3a	158:159	arg1	haptens					172:178	S. flexneri 3a, 4a and 4b haptens	146:178	S. flexneri 3a, 4a and 4b haptens	146:178	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	3	16	theme	mutants	629:635	arg1	collection					612:621	a collection	610:621	a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site,	610:784	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	16	theme	mutants	629:635	arg1	tetrasaccharide					833:847	a lightly protected chemically synthesized tetrasaccharide	790:847	a lightly protected chemically synthesized tetrasaccharide	790:847	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	16	theme	mutants	629:635	arg1	substrate					864:872	an acceptor substrate	852:872	an acceptor substrate	852:872	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	5	17	dep	flexneri	1281:1288	arg1	4b					1311:1312	4b	1311:1312	4b	1311:1312	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	5	17	dep	flexneri	1281:1288	arg1	serotypes					1290:1298	serotypes 3a, 4a and 4b	1290:1312	Shigella flexneri serotypes 3a, 4a and 4b	1272:1312	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	5	17	dep	flexneri	1281:1288	arg1	4a					1304:1305	4a	1304:1305	4a	1304:1305	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	5	17	dep	flexneri	1281:1288	arg1	3a					1300:1301	3a	1300:1301	3a	1300:1301	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	0	18	theme	4a	162:163	arg1	haptens					172:178	S. flexneri 3a, 4a and 4b haptens	146:178	S. flexneri 3a, 4a and 4b haptens	146:178	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	3	19	theme	synthesized	821:831	arg1	collection					612:621	a collection	610:621	a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site,	610:784	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	19	theme	synthesized	821:831	arg1	tetrasaccharide					833:847	a lightly protected chemically synthesized tetrasaccharide	790:847	a lightly protected chemically synthesized tetrasaccharide	790:847	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	19	theme	synthesized	821:831	arg1	substrate					864:872	an acceptor substrate	852:872	an acceptor substrate	852:872	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	0	20	theme	4b	169:170	arg1	haptens					172:178	S. flexneri 3a, 4a and 4b haptens	146:178	S. flexneri 3a, 4a and 4b haptens	146:178	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	3	21	theme	sucrase	664:670	arg1	mutants					629:635	22 mutants	626:635	22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site,	626:784	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	22	theme	protected	800:808	arg1	collection					612:621	a collection	610:621	a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site,	610:784	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	22	theme	protected	800:808	arg1	tetrasaccharide					833:847	a lightly protected chemically synthesized tetrasaccharide	790:847	a lightly protected chemically synthesized tetrasaccharide	790:847	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	22	theme	protected	800:808	arg1	substrate					864:872	an acceptor substrate	852:872	an acceptor substrate	852:872	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	23	theme	ΔN123-GBD-CD2	640:652	arg1	enzyme					676:681	an enzyme	673:681	an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site	673:783	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	23	theme	ΔN123-GBD-CD2	640:652	arg1	sucrase					664:670	ΔN123-GBD-CD2 branching sucrase	640:670	ΔN123-GBD-CD2 branching sucrase	640:670	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	24	theme	product	912:918	arg1	specificities					920:932	altered glycosylation product specificities	890:932	altered glycosylation product specificities	890:932	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	2	25	theme	reaction	514:521	arg1	regioselectivity					476:491	altered regioselectivity	468:491	altered regioselectivity of the glycosylation reaction	468:521	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	3	26	theme	acceptor	855:862	arg1	collection					612:621	a collection	610:621	a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site,	610:784	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	26	theme	acceptor	855:862	arg1	tetrasaccharide					833:847	a lightly protected chemically synthesized tetrasaccharide	790:847	a lightly protected chemically synthesized tetrasaccharide	790:847	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	26	theme	acceptor	855:862	arg1	substrate					864:872	an acceptor substrate	852:872	an acceptor substrate	852:872	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	6	27	theme	active	1406:1411	arg1	site					1413:1416	the active site	1402:1416	the active site	1402:1416	Furthermore, their synthesis was shown to be controlled by the mutations introduced in the active site, driving the glucosylation toward one extremity or the other of the tetrasaccharide acceptor.					
33510212	3	28	theme	altered	890:896	arg1	specificities					920:932	altered glycosylation product specificities	890:932	altered glycosylation product specificities	890:932	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	7	29	theme	molecular	1619:1627	arg1	simulations					1638:1648	extensive molecular dynamics simulations	1609:1648	extensive molecular dynamics simulations	1609:1648	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	0	30	theme	pentasaccharide	111:125	arg1	units					137:141	pentasaccharide repeating units	111:141	pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens	111:178	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	7	31	with	combination	1670:1680	arg1	analyses					1696:1703	in-depth analyses	1687:1703	in-depth analyses of amino acid residue networks	1687:1734	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	2	32	theme	active	421:426	arg1	site					428:431	the enzyme active site	410:431	the enzyme active site	410:431	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	0	33	theme	units	137:141	arg1	production					97:106	the production	93:106	the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens	93:178	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	9	34	theme	new	1882:1884	arg1	insight					1886:1892	new insight	1882:1892	new insight to further engineer this class of enzymes for the synthesis of carbohydrate components of bacterial haptens	1882:2000	They also provide new insight to further engineer this class of enzymes for the synthesis of carbohydrate components of bacterial haptens.					
33510212	1	35	theme	appropriate	266:276	arg1	tools					288:292	appropriate enzymatic tools	266:292	appropriate enzymatic tools with requisite regio- and stereo-specificities	266:339	The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities.					
33510212	3	36	theme	parental	968:975	arg1	enzyme					977:982	the parental enzyme	964:982	the parental enzyme	964:982	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	4	37	theme	favored	1078:1084	arg1	pentasaccharides					1086:1101	two favored pentasaccharides	1074:1101	two favored pentasaccharides	1074:1101	Six mutants were selected for further characterization as they produce higher amounts of two favored pentasaccharides compared to the parental enzyme and/or new products.					
33510212	1	38	theme	tools	288:292	arg1	lack					258:261	the lack	254:261	the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities	254:339	The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities.					
33510212	7	39	theme	acid	1714:1717	arg1	networks					1727:1734	amino acid residue networks	1708:1734	amino acid residue networks	1708:1734	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	0	40	theme	engineered	45:54	arg1	sucrases					80:87	engineered ΔN123-GBD-CD2 branching sucrases	45:87	engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens	45:178	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	1	41	theme	requisite	299:307	arg1	regio-					309:314	requisite regio-	299:314	requisite regio-	299:314	The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities.					
33510212	4	42	theme	higher	1056:1061	arg1	pentasaccharides					1086:1101	two favored pentasaccharides	1074:1101	two favored pentasaccharides	1074:1101	Six mutants were selected for further characterization as they produce higher amounts of two favored pentasaccharides compared to the parental enzyme and/or new products.					
33510212	4	42	theme	higher	1056:1061	arg1	amounts					1063:1069	higher amounts	1056:1069	higher amounts of two favored pentasaccharides	1056:1101	Six mutants were selected for further characterization as they produce higher amounts of two favored pentasaccharides compared to the parental enzyme and/or new products.					
33510212	2	43	theme	carbohydrate-active	357:375	arg1	enzymes					377:383	carbohydrate-active enzymes	357:383	carbohydrate-active enzymes	357:383	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	0	44	theme	branching	70:78	arg1	sucrases					80:87	engineered ΔN123-GBD-CD2 branching sucrases	45:87	engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens	45:178	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	5	45	theme	high	1206:1209	arg1	interest					1211:1218	high interest	1206:1218	high interest	1206:1218	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	9	46	theme	enzymes	1928:1934	arg1	class					1919:1923	this class	1914:1923	this class of enzymes	1914:1934	They also provide new insight to further engineer this class of enzymes for the synthesis of carbohydrate components of bacterial haptens.					
33510212	5	47	theme	Shigella	1272:1279	arg1	flexneri					1281:1288	Shigella flexneri serotypes 3a, 4a and 4b	1272:1312	Shigella flexneri serotypes 3a, 4a and 4b	1272:1312	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	7	48	theme	molecular	1528:1536	arg1	determinants					1538:1549	the molecular determinants	1524:1549	the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity	1524:1606	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	4	49	theme	new	1142:1144	arg1	products					1146:1153	the parental enzyme and/or new products	1115:1153	products	1146:1153	Six mutants were selected for further characterization as they produce higher amounts of two favored pentasaccharides compared to the parental enzyme and/or new products.					
33510212	3	50	theme	Glycoside	692:700	arg1	family					712:717	the Glycoside Hydrolase family 70	688:720	the Glycoside Hydrolase family 70	688:720	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	6	51	theme	tetrasaccharide	1486:1500	arg1	acceptor					1502:1509	the tetrasaccharide acceptor	1482:1509	the tetrasaccharide acceptor	1482:1509	Furthermore, their synthesis was shown to be controlled by the mutations introduced in the active site, driving the glucosylation toward one extremity or the other of the tetrasaccharide acceptor.					
33510212	2	52	from	Engineering	342:352	arg1	particular					389:398	particular	389:398	particular	389:398	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	9	53	theme	components	1970:1979	arg1	synthesis					1944:1952	the synthesis	1940:1952	the synthesis of carbohydrate components of bacterial haptens	1940:2000	They also provide new insight to further engineer this class of enzymes for the synthesis of carbohydrate components of bacterial haptens.					
33510212	5	54	theme	haptens	1261:1267	arg1	they					1223:1226	they	1223:1226	they	1223:1226	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	5	54	theme	haptens	1261:1267	arg1	precursors					1232:1241	precursors	1232:1241	precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b	1232:1312	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	9	55	theme	bacterial	1984:1992	arg1	haptens					1994:2000	bacterial haptens	1984:2000	bacterial haptens	1984:2000	They also provide new insight to further engineer this class of enzymes for the synthesis of carbohydrate components of bacterial haptens.					
33510212	7	56	theme	networks	1727:1734	arg1	analyses					1696:1703	in-depth analyses	1687:1703	in-depth analyses of amino acid residue networks	1687:1734	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	1	57	with	tools	288:292	arg1	regio-					309:314	requisite regio-	299:314	requisite regio-	299:314	The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities.					
33510212	1	57	with	tools	288:292	arg1	stereo-specificities					320:339	stereo-specificities	320:339	stereo-specificities	320:339	The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities.					
33510212	4	58	theme	further	1015:1021	arg1	characterization					1023:1038	further characterization	1015:1038	further characterization	1015:1038	Six mutants were selected for further characterization as they produce higher amounts of two favored pentasaccharides compared to the parental enzyme and/or new products.					
33510212	2	59	from	particular	389:398	arg1	Engineering					342:352	Engineering	342:352	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site,	342:432	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	0	60	theme	flexneri	149:156	arg1	3a					158:159	S. flexneri 3a	146:159	S. flexneri 3a	146:159	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	7	61	theme	in-depth	1687:1694	arg1	analyses					1696:1703	in-depth analyses	1687:1703	in-depth analyses of amino acid residue networks	1687:1734	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	5	62	dep	serotypes	1290:1298	arg1	4b					1311:1312	4b	1311:1312	4b	1311:1312	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	5	62	dep	serotypes	1290:1298	arg1	serotypes					1290:1298	serotypes 3a, 4a and 4b	1290:1312	Shigella flexneri serotypes 3a, 4a and 4b	1272:1312	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	5	62	dep	serotypes	1290:1298	arg1	4a					1304:1305	4a	1304:1305	4a	1304:1305	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	5	62	dep	serotypes	1290:1298	arg1	3a					1300:1301	3a	1300:1301	3a	1300:1301	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	8	63	theme	conformational	1823:1836	arg1	flexibility					1838:1848	conformational flexibility	1823:1848	conformational flexibility	1823:1848	Our findings help to understand the inter-relationships between the enzyme structure, conformational flexibility and activity.					
33510212	0	64	theme	haptens	172:178	arg1	units					137:141	pentasaccharide repeating units	111:141	pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens	111:178	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	3	65	theme	branching	654:662	arg1	enzyme					676:681	an enzyme	673:681	an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site	673:783	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	65	theme	branching	654:662	arg1	sucrase					664:670	ΔN123-GBD-CD2 branching sucrase	640:670	ΔN123-GBD-CD2 branching sucrase	640:670	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	2	66	with	catalysts	453:461	arg1	regioselectivity					476:491	altered regioselectivity	468:491	altered regioselectivity of the glycosylation reaction	468:521	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	7	67	theme	dynamics	1629:1636	arg1	simulations					1638:1648	extensive molecular dynamics simulations	1609:1648	extensive molecular dynamics simulations	1609:1648	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	1	68	theme	oligosaccharides	216:231	arg1	synthesis					203:211	The (chemo-)enzymatic synthesis	181:211	The (chemo-)enzymatic synthesis of oligosaccharides	181:231	The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities.					
33510212	7	69	theme	extensive	1609:1617	arg1	simulations					1638:1648	extensive molecular dynamics simulations	1609:1648	extensive molecular dynamics simulations	1609:1648	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	2	70	theme	glycosylation	500:512	arg1	reaction					514:521	the glycosylation reaction	496:521	the glycosylation reaction	496:521	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	3	71	contain	containing	723:732	arg2	mutations					756:764	between one and three mutations	734:764	between one and three mutations	734:764	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	3	71	contain	containing	723:732	arg1	family					712:717	the Glycoside Hydrolase family 70	688:720	the Glycoside Hydrolase family 70	688:720	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	0	72	theme	repeating	127:135	arg1	units					137:141	pentasaccharide repeating units	111:141	pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens	111:178	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	3	73	theme	glycosylation	898:910	arg1	specificities					920:932	altered glycosylation product specificities	890:932	altered glycosylation product specificities	890:932	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	7	74	theme	regioselectivity	1591:1606	arg1	change					1567:1572	the change	1563:1572	the change of ΔN123-GBD-CD2 regioselectivity	1563:1606	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	2	75	theme	enzyme	414:419	arg1	site					428:431	the enzyme active site	410:431	the enzyme active site	410:431	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	0	76	theme	substrate	12:20	arg1	regioselectivity					22:37	substrate regioselectivity	12:37	substrate regioselectivity	12:37	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	5	77	theme	produced	1160:1167	arg1	pentasaccharides					1169:1184	The produced pentasaccharides	1156:1184	The produced pentasaccharides	1156:1184	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	1	78	theme	enzymatic	278:286	arg1	tools					288:292	appropriate enzymatic tools	266:292	appropriate enzymatic tools with requisite regio- and stereo-specificities	266:339	The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities.					
33510212	0	79	theme	ΔN123-GBD-CD2	56:68	arg1	sucrases					80:87	engineered ΔN123-GBD-CD2 branching sucrases	45:87	engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens	45:178	Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens.					
33510212	6	80	dep	one	1452:1454	arg1	extremity					1456:1464	extremity	1456:1464	one extremity	1452:1464	Furthermore, their synthesis was shown to be controlled by the mutations introduced in the active site, driving the glucosylation toward one extremity or the other of the tetrasaccharide acceptor.					
33510212	7	81	theme	ΔN123-GBD-CD2	1577:1589	arg1	regioselectivity					1591:1606	ΔN123-GBD-CD2 regioselectivity	1577:1606	ΔN123-GBD-CD2 regioselectivity	1577:1606	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	7	82	theme	residue	1719:1725	arg1	networks					1727:1734	amino acid residue networks	1708:1734	amino acid residue networks	1708:1734	To identify the molecular determinants involved in the change of ΔN123-GBD-CD2 regioselectivity, extensive molecular dynamics simulations were carried out in combination with in-depth analyses of amino acid residue networks.					
33510212	2	83	theme	altered	468:474	arg1	regioselectivity					476:491	altered regioselectivity	468:491	altered regioselectivity of the glycosylation reaction	468:521	Engineering of carbohydrate-active enzymes, in particular targeting the enzyme active site, has notably led to catalysts with altered regioselectivity of the glycosylation reaction thereby enabling to extend the repertoire of enzymes for carbohydrate synthesis.					
33510212	3	84	theme	Hydrolase	702:710	arg1	family					712:717	the Glycoside Hydrolase family 70	688:720	the Glycoside Hydrolase family 70	688:720	Using a collection of 22 mutants of ΔN123-GBD-CD2 branching sucrase, an enzyme from the Glycoside Hydrolase family 70, containing between one and three mutations in the active site, and a lightly protected chemically synthesized tetrasaccharide as an acceptor substrate, we showed that altered glycosylation product specificities could be achieved compared to the parental enzyme.					
33510212	4	85	theme	parental	1119:1126	arg1	enzyme					1128:1133	the parental enzyme and/or new products	1115:1153	enzyme	1128:1133	Six mutants were selected for further characterization as they produce higher amounts of two favored pentasaccharides compared to the parental enzyme and/or new products.					
33510212	5	86	theme	flexneri	1281:1288	arg1	haptens					1261:1267	representative haptens	1246:1267	representative haptens of Shigella flexneri serotypes 3a, 4a and 4b	1246:1312	The produced pentasaccharides were shown to be of high interest as they are precursors of representative haptens of Shigella flexneri serotypes 3a, 4a and 4b.					
33510212	6	87	theme	acceptor	1502:1509	arg1	acceptor					1502:1509	the tetrasaccharide acceptor	1482:1509	the tetrasaccharide acceptor	1482:1509	Furthermore, their synthesis was shown to be controlled by the mutations introduced in the active site, driving the glucosylation toward one extremity or the other of the tetrasaccharide acceptor.					
33510212	6	87	theme	acceptor	1502:1509	arg1	one					1452:1454	one	1452:1454	one	1452:1454	Furthermore, their synthesis was shown to be controlled by the mutations introduced in the active site, driving the glucosylation toward one extremity or the other of the tetrasaccharide acceptor.					
34236658	4	0	theme	default	538:544	arg1	choices					546:552	The default choices	534:552	The default choices	534:552	The default choices are often too broad, and a fastidious literature search to properly target this selection can be avoided.					
34236658	4	0	theme	default	538:544	arg1	broad					568:572	broad	568:572	broad	568:572	The default choices are often too broad, and a fastidious literature search to properly target this selection can be avoided.					
34236658	5	1	theme	Compozitor	704:713	arg1	use					686:688	the use	682:688	the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions	682:933	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	3	2	gly	glycopeptide	440:451	arg2	glycopeptide					440:451	automated glycopeptide identification software	430:475	automated glycopeptide identification software	430:475	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	2	3	theme	software	282:289	arg1	solutions					291:299	software solutions	282:299	software solutions	282:299	An extensive range of software solutions is available, but the reproducibility of mass spectrometry data processing remains challenging.					
34236658	5	4	from	information	735:745	arg1	glycosylation					750:762	glycosylation	750:762	glycosylation	750:762	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	5	5	theme	given	769:773	arg1	tissue					775:780	a given tissue or cell line	767:793	tissue	775:780	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	3	6	theme	file	528:531	arg1	issues					412:417	the key issues	404:417	the key issues in running automated glycopeptide identification software	404:475	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	3	6	theme	file	528:531	arg1	selection					484:492	the selection	480:492	the selection of a reference glycan composition file	480:531	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	3	6	theme	file	528:531	arg1	One					397:399	One	397:399	One	397:399	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	1	7	from	past	175:178	arg1	benefits					161:168	its current development benefits	137:168	its current development benefits from past experience	137:189	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
34236658	1	7	from	past	175:178	arg1	rise					128:131	rise	128:131	rise	128:131	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
34236658	3	8	theme	automated	430:438	arg1	software					468:475	automated glycopeptide identification software	430:475	automated glycopeptide identification software	430:475	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	5	9	theme	search	880:885	arg1	engines					887:893	search engines	880:893	search engines accommodating user-defined compositions	880:933	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	3	10	theme	issues	412:417	arg1	issues					412:417	the key issues	404:417	the key issues in running automated glycopeptide identification software	404:475	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	3	10	theme	issues	412:417	arg1	selection					484:492	the selection	480:492	the selection of a reference glycan composition file	480:531	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	3	10	theme	issues	412:417	arg1	One					397:399	One	397:399	One	397:399	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	4	11	theme	literature	592:601	arg1	search					603:608	a fastidious literature search	579:608	a fastidious literature search to properly target this selection	579:642	The default choices are often too broad, and a fastidious literature search to properly target this selection can be avoided.					
34236658	1	12	from	benefits	161:168	arg1	proteomics					194:203	proteomics	194:203	proteomics	194:203	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
34236658	1	12	from	benefits	161:168	arg1	particular					209:218	particular	209:218	particular	209:218	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
34236658	2	13	theme	mass	342:345	arg1	spectrometry					347:358	mass spectrometry	342:358	mass spectrometry data processing	342:374	An extensive range of software solutions is available, but the reproducibility of mass spectrometry data processing remains challenging.					
34236658	5	14	theme	engines	887:893	arg1	majority					868:875	the majority	864:875	the majority of search engines accommodating user-defined compositions	864:933	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	0	15	theme	Glycan	0:5	arg1	Compositions					7:18	Glycan Compositions	0:18	Glycan Compositions with GlyConnect Compozitor to	0:48	Glycan Compositions with GlyConnect Compozitor to Enhance Glycopeptide Identification.					
34236658	4	16	theme	fastidious	581:590	arg1	search					603:608	a fastidious literature search	579:608	a fastidious literature search to properly target this selection	579:642	The default choices are often too broad, and a fastidious literature search to properly target this selection can be avoided.					
34236658	3	17	theme	reference	499:507	arg1	file					528:531	a reference glycan composition file	497:531	a reference glycan composition file	497:531	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	3	18	theme	key	408:410	arg1	issues					412:417	the key issues	404:417	the key issues in running automated glycopeptide identification software	404:475	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	5	19	from	input	855:859	arg1	majority					868:875	the majority	864:875	the majority of search engines accommodating user-defined compositions	864:933	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	1	20	dep	past	175:178	arg1	experience					180:189	experience	180:189	experience	180:189	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
34236658	3	21	theme	glycan	509:514	arg1	file					528:531	a reference glycan composition file	497:531	a reference glycan composition file	497:531	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	3	22	theme	glycopeptide	440:451	arg1	software					468:475	automated glycopeptide identification software	430:475	automated glycopeptide identification software	430:475	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	2	23	theme	processing	365:374	arg1	reproducibility					323:337	the reproducibility	319:337	the reproducibility of mass spectrometry data processing	319:374	An extensive range of software solutions is available, but the reproducibility of mass spectrometry data processing remains challenging.					
34236658	2	24	theme	extensive	263:271	arg1	available					304:312	available	304:312	available	304:312	An extensive range of software solutions is available, but the reproducibility of mass spectrometry data processing remains challenging.					
34236658	2	24	theme	extensive	263:271	arg1	range					273:277	An extensive range	260:277	An extensive range of software solutions	260:299	An extensive range of software solutions is available, but the reproducibility of mass spectrometry data processing remains challenging.					
34236658	3	25	theme	identification	453:466	arg1	software					468:475	automated glycopeptide identification software	430:475	automated glycopeptide identification software	430:475	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	5	26	theme	appropriate	808:818	arg1	input					855:859	input	855:859	input	855:859	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	5	26	theme	appropriate	808:818	arg1	set					839:841	an appropriate glycan composition set	805:841	an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions	805:933	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	5	27	theme	glycan	820:825	arg1	input					855:859	input	855:859	input	855:859	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	5	27	theme	glycan	820:825	arg1	set					839:841	an appropriate glycan composition set	805:841	an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions	805:933	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	5	28	theme	user-defined	909:920	arg1	compositions					922:933	user-defined compositions	909:933	user-defined compositions	909:933	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	5	29	theme	composition	827:837	arg1	input					855:859	input	855:859	input	855:859	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	5	29	theme	composition	827:837	arg1	set					839:841	an appropriate glycan composition set	805:841	an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions	805:933	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	0	30	theme	Glycopeptide	58:69	arg1	Identification					71:84	Glycopeptide Identification	58:84	Glycopeptide Identification	58:84	Glycan Compositions with GlyConnect Compozitor to Enhance Glycopeptide Identification.					
34236658	3	31	theme	composition	516:526	arg1	file					528:531	a reference glycan composition file	497:531	a reference glycan composition file	497:531	One of the key issues in running automated glycopeptide identification software is the selection of a reference glycan composition file.					
34236658	0	32	with	Compositions	7:18	arg1	Compozitor to					36:48	Compozitor to	36:48	Compozitor to	36:48	Glycan Compositions with GlyConnect Compozitor to Enhance Glycopeptide Identification.					
34236658	1	33	theme	current	141:147	arg1	benefits					161:168	its current development benefits	137:168	its current development benefits from past experience	137:189	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
34236658	1	34	from	rise	128:131	arg1	proteomics					194:203	proteomics	194:203	proteomics	194:203	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
34236658	1	34	from	rise	128:131	arg1	particular					209:218	particular	209:218	particular	209:218	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
34236658	2	35	theme	solutions	291:299	arg1	available					304:312	available	304:312	available	304:312	An extensive range of software solutions is available, but the reproducibility of mass spectrometry data processing remains challenging.					
34236658	2	35	theme	solutions	291:299	arg1	range					273:277	An extensive range	260:277	An extensive range of software solutions	260:299	An extensive range of software solutions is available, but the reproducibility of mass spectrometry data processing remains challenging.					
34236658	1	36	theme	development	149:159	arg1	benefits					161:168	its current development benefits	137:168	its current development benefits from past experience	137:189	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
34236658	5	37	theme	relevant	726:733	arg1	information					735:745	relevant information	726:745	relevant information on glycosylation	726:762	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	1	38	dep	bioinformatics	238:251	arg1	needs					253:257	needs	253:257	needs	253:257	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
34236658	2	39	theme	spectrometry	347:358	arg1	processing					365:374	mass spectrometry data processing	342:374	mass spectrometry data processing	342:374	An extensive range of software solutions is available, but the reproducibility of mass spectrometry data processing remains challenging.					
34236658	2	40	theme	data	360:363	arg1	processing					365:374	mass spectrometry data processing	342:374	mass spectrometry data processing	342:374	An extensive range of software solutions is available, but the reproducibility of mass spectrometry data processing remains challenging.					
34236658	5	41	theme	cell	785:788	arg1	line					790:793	a given tissue or cell line	767:793	line	790:793	This chapter suggests the use of GlyConnect Compozitor to collect relevant information on glycosylation in a given tissue or cell line and shape an appropriate glycan composition set that can be input in the majority of search engines accommodating user-defined compositions.					
34236658	1	42	dep	rise	128:131	arg1	the					124:126	the	124:126	the	124:126	Glycoproteomics is unquestionably on the rise and its current development benefits from past experience in proteomics, in particular when attending to bioinformatics needs.					
33734311	7	0	theme	neutrophils	1282:1292	arg1	proteins					1264:1271	lysosomal proteins	1254:1271	lysosomal proteins of human neutrophils	1254:1292	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	7	0	theme	neutrophils	1282:1292	arg1	types					1306:1310	several types	1298:1310	several types of cancers	1298:1321	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	9	1	theme	aglycone	1469:1476	arg1	modification					1478:1489	Fmoc aglycone modification	1464:1489	Fmoc aglycone modification	1464:1489	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	1	2	theme	sole	168:171	arg1	α1,6-fucosyltransferase					130:152	The α1,6-fucosyltransferase	126:152	The α1,6-fucosyltransferase	126:152	The α1,6-fucosyltransferase, FUT8, is the sole enzyme catalyzing the core-fucosylation of N-glycoproteins in mammalian systems.					
33734311	1	2	theme	sole	168:171	arg1	enzyme					173:178	the sole enzyme	164:178	the sole enzyme catalyzing the core-fucosylation of N-glycoproteins in mammalian systems	164:251	The α1,6-fucosyltransferase, FUT8, is the sole enzyme catalyzing the core-fucosylation of N-glycoproteins in mammalian systems.					
33734311	0	3	theme	α1,6-fucosyltransferase	94:116	arg1	acceptability					77:89	the glycan substrate acceptability	56:89	the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8)	56:123	Appropriate aglycone modification significantly expands the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8).					
33734311	5	4	theme	9-fluorenylmethyloxycarbonyl	939:966	arg1	moiety					975:980	a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety	937:980	a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety	937:980	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	2	5	theme	N-glycan	387:394	arg1	substrates					396:405	N-glycan substrates	387:405	N-glycan substrates	387:405	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	9	6	theme	Fmoc	1464:1467	arg1	modification					1478:1489	Fmoc aglycone modification	1464:1489	Fmoc aglycone modification	1464:1489	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	4	7	contain	containing	718:727	arg1	N-glycans					708:716	N-glycans	708:716	N-glycans containing different aglycones	708:747	We describe here a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones.					
33734311	4	7	contain	containing	718:727	arg2	aglycones					739:747	different aglycones	729:747	different aglycones	729:747	We describe here a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones.					
33734311	7	8	gly	core-fucosylated	1192:1207	arg1	paucimannosylation					1209:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	7	8	gly	core-fucosylated	1192:1207	arg1	feature					1243:1249	a prominent feature	1231:1249	a prominent feature of lysosomal proteins of human neutrophils and several types of cancers	1231:1321	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	2	9	from	moiety	353:358	arg1	arm					380:382	the Man-α1,3-Man arm	363:382	the Man-α1,3-Man arm of N-glycan substrates	363:405	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	2	9	from	moiety	353:358	arg1	substrates					396:405	N-glycan substrates	387:405	N-glycan substrates	387:405	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	7	10	theme	human	1276:1280	arg1	neutrophils					1282:1292	human neutrophils	1276:1292	human neutrophils	1276:1292	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	9	11	theme	fucosylation	1668:1679	arg1	efficiency					1639:1648	the overall efficiency	1627:1648	the overall efficiency of FUT8 catalyzed fucosylation	1627:1679	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	1	12	gly	core-fucosylation	195:211	arg1	N-glycoproteins					216:230	N-glycoproteins	216:230	N-glycoproteins	216:230	The α1,6-fucosyltransferase, FUT8, is the sole enzyme catalyzing the core-fucosylation of N-glycoproteins in mammalian systems.					
33734311	1	12	gly	core-fucosylation	195:211	arg1	systems					245:251	mammalian systems	235:251	mammalian systems	235:251	The α1,6-fucosyltransferase, FUT8, is the sole enzyme catalyzing the core-fucosylation of N-glycoproteins in mammalian systems.					
33734311	7	13	theme	cancers	1315:1321	arg1	proteins					1264:1271	lysosomal proteins	1254:1271	lysosomal proteins of human neutrophils	1254:1292	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	7	13	theme	cancers	1315:1321	arg1	types					1306:1310	several types	1298:1310	several types of cancers	1298:1321	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	2	14	theme	substrates	396:405	arg1	arm					380:382	the Man-α1,3-Man arm	363:382	the Man-α1,3-Man arm of N-glycan substrates	363:405	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	2	14	theme	substrates	396:405	arg1	substrates					396:405	N-glycan substrates	387:405	N-glycan substrates	387:405	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	9	15	theme	KM	1546:1547	arg1	value					1549:1553	the KM value	1542:1553	the KM value depending on the nature of the substrates	1542:1595	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	9	16	theme	turnover	1516:1523	arg1	rate					1525:1528	the turnover rate	1512:1528	the turnover rate	1512:1528	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	4	17	theme	different	729:737	arg1	aglycones					739:747	different aglycones	729:747	different aglycones	729:747	We describe here a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones.					
33734311	8	18	theme	N-glycans	1358:1366	arg1	sialylation					1343:1353	sialylation	1343:1353	sialylation of N-glycans	1343:1366	We also found that sialylation of N-glycans significantly reduced their activity as a substrate of FUT8.					
33734311	7	19	theme	prominent	1233:1241	arg1	paucimannosylation					1209:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	7	19	theme	prominent	1233:1241	arg1	feature					1243:1249	a prominent feature	1231:1249	a prominent feature of lysosomal proteins of human neutrophils and several types of cancers	1231:1321	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	4	20	theme	substrate	661:669	arg1	specificity					671:681	the substrate specificity	657:681	the substrate specificity of FUT8 using a range of N-glycans containing different aglycones	657:747	We describe here a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones.					
33734311	3	21	gly	fucosylate	556:565	arg1	Man5GlcNAc2					567:577	Man5GlcNAc2	567:577	Man5GlcNAc2	567:577	In contrast, we recently demonstrated that, in a proper protein context, FUT8 could also fucosylate Man5GlcNAc2 without a GlcNAc at the non-reducing end.					
33734311	5	22	mod	modified	923:930	arg1	moiety					913:918	the aglycone moiety	900:918	the aglycone moiety	900:918	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	5	22	mod	modified	923:930	arg3	moiety					975:980	a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety	937:980	a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety	937:980	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	7	23	theme	proteins	1264:1271	arg1	paucimannosylation					1209:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	7	23	theme	proteins	1264:1271	arg1	feature					1243:1249	a prominent feature	1231:1249	a prominent feature of lysosomal proteins of human neutrophils and several types of cancers	1231:1321	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	1	24	gly	N-glycoproteins	216:230	arg1	N-glycoproteins					216:230	N-glycoproteins	216:230	N-glycoproteins	216:230	The α1,6-fucosyltransferase, FUT8, is the sole enzyme catalyzing the core-fucosylation of N-glycoproteins in mammalian systems.					
33734311	7	25	theme	Such	1187:1190	arg1	paucimannosylation					1209:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	7	25	theme	Such	1187:1190	arg1	feature					1243:1249	a prominent feature	1231:1249	a prominent feature of lysosomal proteins of human neutrophils and several types of cancers	1231:1321	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	4	26	theme	further	640:646	arg1	study					648:652	a further study	638:652	a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones	638:747	We describe here a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones.					
33734311	2	27	theme	FUT8-catalyzed	433:446	arg1	core-fucosylation					448:464	efficient FUT8-catalyzed core-fucosylation	423:464	efficient FUT8-catalyzed core-fucosylation	423:464	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	0	28	theme	aglycone	12:19	arg1	modification					21:32	Appropriate aglycone modification	0:32	Appropriate aglycone modification	0:32	Appropriate aglycone modification significantly expands the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8).					
33734311	5	29	theme	complex-type	811:822	arg1	N-glycans					861:869	highly branched N-glycans	845:869	highly branched N-glycans from chicken ovalbumin	845:892	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	5	29	theme	complex-type	811:822	arg1	N-glycans					824:832	high-mannose and complex-type N-glycans	794:832	high-mannose and complex-type N-glycans	794:832	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	6	30	theme	paucimannose	1121:1132	arg1	structures					1134:1143	paucimannose structures	1121:1143	paucimannose structures	1121:1143	FUT8 could also fucosylate paucimannose structures when they are on glycoprotein substrates.					
33734311	2	31	theme	efficient	423:431	arg1	core-fucosylation					448:464	efficient FUT8-catalyzed core-fucosylation	423:464	efficient FUT8-catalyzed core-fucosylation	423:464	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	0	32	theme	Appropriate	0:10	arg1	modification					21:32	Appropriate aglycone modification	0:32	Appropriate aglycone modification	0:32	Appropriate aglycone modification significantly expands the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8).					
33734311	3	33	theme	non-reducing	603:614	arg1	end					616:618	the non-reducing end	599:618	the non-reducing end	599:618	In contrast, we recently demonstrated that, in a proper protein context, FUT8 could also fucosylate Man5GlcNAc2 without a GlcNAc at the non-reducing end.					
33734311	6	34	gly	glycoprotein	1162:1173	arg1	glycoprotein					1162:1173	glycoprotein substrates	1162:1184	glycoprotein substrates	1162:1184	FUT8 could also fucosylate paucimannose structures when they are on glycoprotein substrates.					
33734311	9	35	theme	FUT8	1653:1656	arg1	fucosylation					1668:1679	FUT8 catalyzed fucosylation	1653:1679	FUT8 catalyzed fucosylation	1653:1679	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	10	36	theme	acceptor	1785:1792	arg1	specificity					1804:1814	the acceptor substrate specificity	1781:1814	the acceptor substrate specificity of FUT8 beyond what has previously been thought	1781:1862	Our results indicate that an appropriate aglycone context of N-glycans could significantly broaden the acceptor substrate specificity of FUT8 beyond what has previously been thought.					
33734311	2	37	theme	β1,2-GlcNAc	341:351	arg1	moiety					353:358	a terminal β1,2-GlcNAc moiety	330:358	a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates	330:405	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	1	38	theme	N-glycoproteins	216:230	arg1	core-fucosylation					195:211	the core-fucosylation	191:211	the core-fucosylation of N-glycoproteins in mammalian systems	191:251	The α1,6-fucosyltransferase, FUT8, is the sole enzyme catalyzing the core-fucosylation of N-glycoproteins in mammalian systems.					
33734311	5	39	theme	chicken	876:882	arg1	ovalbumin					884:892	chicken ovalbumin	876:892	chicken ovalbumin	876:892	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	9	40	theme	overall	1631:1637	arg1	efficiency					1639:1648	the overall efficiency	1627:1648	the overall efficiency of FUT8 catalyzed fucosylation	1627:1679	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	2	41	theme	terminal	332:339	arg1	moiety					353:358	a terminal β1,2-GlcNAc moiety	330:358	a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates	330:405	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	3	42	theme	proper	516:521	arg1	context					531:537	a proper protein context	514:537	a proper protein context	514:537	In contrast, we recently demonstrated that, in a proper protein context, FUT8 could also fucosylate Man5GlcNAc2 without a GlcNAc at the non-reducing end.					
33734311	10	43	theme	aglycone	1723:1730	arg1	context					1732:1738	an appropriate aglycone context	1708:1738	an appropriate aglycone context of N-glycans	1708:1751	Our results indicate that an appropriate aglycone context of N-glycans could significantly broaden the acceptor substrate specificity of FUT8 beyond what has previously been thought.					
33734311	4	44	theme	FUT8	686:689	arg1	specificity					671:681	the substrate specificity	657:681	the substrate specificity of FUT8 using a range of N-glycans containing different aglycones	657:747	We describe here a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones.					
33734311	5	45	theme	GlcNAc	1055:1060	arg1	moiety					1062:1067	the terminal GlcNAc moiety	1042:1067	the terminal GlcNAc moiety on the Man-α1,3-Man arm	1042:1091	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	5	46	from	moiety	1062:1067	arg1	arm					1089:1091	the Man-α1,3-Man arm	1072:1091	the Man-α1,3-Man arm	1072:1091	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	1	47	theme	mammalian	235:243	arg1	systems					245:251	mammalian systems	235:251	mammalian systems	235:251	The α1,6-fucosyltransferase, FUT8, is the sole enzyme catalyzing the core-fucosylation of N-glycoproteins in mammalian systems.					
33734311	5	48	theme	aglycone	904:911	arg1	moiety					913:918	the aglycone moiety	900:918	the aglycone moiety	900:918	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	9	49	theme	Kinetic	1429:1435	arg1	analysis					1437:1444	Kinetic analysis	1429:1444	Kinetic analysis	1429:1444	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	10	50	theme	appropriate	1711:1721	arg1	context					1732:1738	an appropriate aglycone context	1708:1738	an appropriate aglycone context of N-glycans	1708:1751	Our results indicate that an appropriate aglycone context of N-glycans could significantly broaden the acceptor substrate specificity of FUT8 beyond what has previously been thought.					
33734311	9	51	theme	catalyzed	1658:1666	arg1	fucosylation					1668:1679	FUT8 catalyzed fucosylation	1653:1679	FUT8 catalyzed fucosylation	1653:1679	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	0	52	theme	substrate	67:75	arg1	acceptability					77:89	the glycan substrate acceptability	56:89	the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8)	56:123	Appropriate aglycone modification significantly expands the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8).					
33734311	6	53	gly	fucosylate	1110:1119	arg1	structures					1134:1143	paucimannose structures	1121:1143	paucimannose structures	1121:1143	FUT8 could also fucosylate paucimannose structures when they are on glycoprotein substrates.					
33734311	7	54	theme	types	1306:1310	arg1	paucimannosylation					1209:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	7	54	theme	types	1306:1310	arg1	feature					1243:1249	a prominent feature	1231:1249	a prominent feature of lysosomal proteins of human neutrophils and several types of cancers	1231:1321	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	2	55	theme	Previous	254:261	arg1	studies					263:269	Previous studies	254:269	Previous studies using free N-glycans as acceptor substrates	254:313	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	6	56	theme	glycoprotein	1162:1173	arg1	substrates					1175:1184	glycoprotein substrates	1162:1184	glycoprotein substrates	1162:1184	FUT8 could also fucosylate paucimannose structures when they are on glycoprotein substrates.					
33734311	5	57	theme	Fmoc	969:972	arg1	moiety					975:980	a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety	937:980	a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety	937:980	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	0	58	theme	glycan	60:65	arg1	acceptability					77:89	the glycan substrate acceptability	56:89	the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8)	56:123	Appropriate aglycone modification significantly expands the glycan substrate acceptability of α1,6-fucosyltransferase (FUT8).					
33734311	9	59	theme	substrates	1586:1595	arg1	nature					1572:1577	the nature	1568:1577	the nature of the substrates	1568:1595	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	5	60	theme	Man-α1,3-Man	1076:1087	arg1	arm					1089:1091	the Man-α1,3-Man arm	1072:1091	the Man-α1,3-Man arm	1072:1091	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	5	61	from	ovalbumin	884:892	arg1	N-glycans					861:869	highly branched N-glycans	845:869	highly branched N-glycans from chicken ovalbumin	845:892	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	5	62	theme	terminal	1046:1053	arg1	moiety					1062:1067	the terminal GlcNAc moiety	1042:1067	the terminal GlcNAc moiety on the Man-α1,3-Man arm	1042:1091	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	7	63	theme	core-fucosylated	1192:1207	arg1	paucimannosylation					1209:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation	1187:1226	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	7	63	theme	core-fucosylated	1192:1207	arg1	feature					1243:1249	a prominent feature	1231:1249	a prominent feature of lysosomal proteins of human neutrophils and several types of cancers	1231:1321	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	2	64	theme	acceptor	295:302	arg1	N-glycans					282:290	free N-glycans	277:290	free N-glycans	277:290	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	2	64	theme	acceptor	295:302	arg1	substrates					304:313	acceptor substrates	295:313	acceptor substrates	295:313	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	9	65	from	value	1549:1553	arg1	nature					1572:1577	the nature	1568:1577	the nature of the substrates	1568:1595	Kinetic analysis demonstrated that Fmoc aglycone modification could either improve the turnover rate or decrease the KM value depending on the nature of the substrates, thus significantly enhancing the overall efficiency of FUT8 catalyzed fucosylation.					
33734311	2	66	theme	Man-α1,3-Man	367:378	arg1	arm					380:382	the Man-α1,3-Man arm	363:382	the Man-α1,3-Man arm of N-glycan substrates	363:405	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	2	66	theme	Man-α1,3-Man	367:378	arg1	substrates					396:405	N-glycan substrates	387:405	N-glycan substrates	387:405	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	5	67	theme	suitable	990:997	arg1	context					1015:1021	a suitable peptide/protein context	988:1021	a suitable peptide/protein context	988:1021	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	5	68	theme	branched	852:859	arg1	N-glycans					861:869	highly branched N-glycans	845:869	highly branched N-glycans from chicken ovalbumin	845:892	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	8	69	theme	FUT8	1423:1426	arg1	activity					1396:1403	their activity	1390:1403	their activity	1390:1403	We also found that sialylation of N-glycans significantly reduced their activity as a substrate of FUT8.					
33734311	8	69	theme	FUT8	1423:1426	arg1	substrate					1410:1418	a substrate	1408:1418	a substrate of FUT8	1408:1426	We also found that sialylation of N-glycans significantly reduced their activity as a substrate of FUT8.					
33734311	1	70	from	core-fucosylation	195:211	arg1	systems					245:251	mammalian systems	235:251	mammalian systems	235:251	The α1,6-fucosyltransferase, FUT8, is the sole enzyme catalyzing the core-fucosylation of N-glycoproteins in mammalian systems.					
33734311	10	71	theme	substrate	1794:1802	arg1	specificity					1804:1814	the acceptor substrate specificity	1781:1814	the acceptor substrate specificity of FUT8 beyond what has previously been thought	1781:1862	Our results indicate that an appropriate aglycone context of N-glycans could significantly broaden the acceptor substrate specificity of FUT8 beyond what has previously been thought.					
33734311	3	72	from	end	616:618	arg1	GlcNAc					589:594	a GlcNAc	587:594	a GlcNAc at the non-reducing end	587:618	In contrast, we recently demonstrated that, in a proper protein context, FUT8 could also fucosylate Man5GlcNAc2 without a GlcNAc at the non-reducing end.					
33734311	3	73	theme	protein	523:529	arg1	context					531:537	a proper protein context	514:537	a proper protein context	514:537	In contrast, we recently demonstrated that, in a proper protein context, FUT8 could also fucosylate Man5GlcNAc2 without a GlcNAc at the non-reducing end.					
33734311	7	74	theme	several	1298:1304	arg1	types					1306:1310	several types	1298:1310	several types of cancers	1298:1321	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	8	75	gly	sialylation	1343:1353	arg1	N-glycans					1358:1366	N-glycans	1358:1366	N-glycans	1358:1366	We also found that sialylation of N-glycans significantly reduced their activity as a substrate of FUT8.					
33734311	4	76	theme	specificity	671:681	arg1	study					648:652	a further study	638:652	a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones	638:747	We describe here a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones.					
33734311	5	77	theme	peptide/protein	999:1013	arg1	context					1015:1021	a suitable peptide/protein context	988:1021	a suitable peptide/protein context	988:1021	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	10	78	theme	N-glycans	1743:1751	arg1	context					1732:1738	an appropriate aglycone context	1708:1738	an appropriate aglycone context of N-glycans	1708:1751	Our results indicate that an appropriate aglycone context of N-glycans could significantly broaden the acceptor substrate specificity of FUT8 beyond what has previously been thought.					
33734311	5	79	theme	high-mannose	794:805	arg1	N-glycans					861:869	highly branched N-glycans	845:869	highly branched N-glycans from chicken ovalbumin	845:892	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	5	79	theme	high-mannose	794:805	arg1	N-glycans					824:832	high-mannose and complex-type N-glycans	794:832	high-mannose and complex-type N-glycans	794:832	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	7	80	theme	lysosomal	1254:1262	arg1	proteins					1264:1271	lysosomal proteins	1254:1271	lysosomal proteins of human neutrophils	1254:1292	Such core-fucosylated paucimannosylation is a prominent feature of lysosomal proteins of human neutrophils and several types of cancers.					
33734311	2	81	theme	free	277:280	arg1	N-glycans					282:290	free N-glycans	277:290	free N-glycans	277:290	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	2	81	theme	free	277:280	arg1	substrates					304:313	acceptor substrates	295:313	acceptor substrates	295:313	Previous studies using free N-glycans as acceptor substrates indicated that a terminal β1,2-GlcNAc moiety on the Man-α1,3-Man arm of N-glycan substrates is required for efficient FUT8-catalyzed core-fucosylation.					
33734311	4	82	theme	N-glycans	708:716	arg1	range					699:703	a range	697:703	a range of N-glycans containing different aglycones	697:747	We describe here a further study of the substrate specificity of FUT8 using a range of N-glycans containing different aglycones.					
33734311	5	83	gly	fucosylate	775:784	arg1	N-glycans					861:869	highly branched N-glycans	845:869	highly branched N-glycans from chicken ovalbumin	845:892	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	5	83	gly	fucosylate	775:784	arg1	N-glycans					824:832	high-mannose and complex-type N-glycans	794:832	high-mannose and complex-type N-glycans	794:832	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	5	83	gly	fucosylate	775:784	arg1	most					786:789	most	786:789	most	786:789	We found that FUT8 could fucosylate most of high-mannose and complex-type N-glycans, including highly branched N-glycans from chicken ovalbumin, when the aglycone moiety is modified with a 9-fluorenylmethyloxycarbonyl (Fmoc) moiety or in a suitable peptide/protein context, even if they lack the terminal GlcNAc moiety on the Man-α1,3-Man arm.					
33734311	10	84	theme	FUT8	1819:1822	arg1	specificity					1804:1814	the acceptor substrate specificity	1781:1814	the acceptor substrate specificity of FUT8 beyond what has previously been thought	1781:1862	Our results indicate that an appropriate aglycone context of N-glycans could significantly broaden the acceptor substrate specificity of FUT8 beyond what has previously been thought.					
34140625	6	0	from	present	961:967	arg1	caldariorum					984:994	Mesotaenium caldariorum	972:994	Mesotaenium caldariorum	972:994	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	1	1	theme	particular	135:144	arg1	significance					159:170	particular evolutionary significance	135:170	particular evolutionary significance	135:170	The charophycean green algae (CGA or basal streptophytes) are of particular evolutionary significance because their ancestors gave rise to land plants.					
34140625	5	2	theme	XyG	654:656	arg1	identification					560:573	the identification	556:573	the identification of orthologs of the genetic machinery to produce XyG	556:626	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	2	theme	XyG	654:656	arg1	identification					636:649	the identification	632:649	b) the identification of XyG in a range of CGA	629:674	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	2	theme	XyG	654:656	arg1	elucidation					699:709	the structural elucidation	684:709	c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA	681:771	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	0	3	from	origin	8:13	arg1	algae					63:67	charophycean green algae	44:67	charophycean green algae	44:67	Ancient origin of fucosylated xyloglucan in charophycean green algae.					
34140625	5	4	theme	orthologs	578:586	arg1	identification					560:573	the identification	556:573	the identification of orthologs of the genetic machinery to produce XyG	556:626	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	4	theme	orthologs	578:586	arg1	identification					636:649	the identification	632:649	b) the identification of XyG in a range of CGA	629:674	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	4	theme	orthologs	578:586	arg1	elucidation					699:709	the structural elucidation	684:709	c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA	681:771	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	1	5	dep	algae	93:97	arg1	streptophytes					113:125	basal streptophytes	107:125	basal streptophytes	107:125	The charophycean green algae (CGA or basal streptophytes) are of particular evolutionary significance because their ancestors gave rise to land plants.					
34140625	1	5	dep	algae	93:97	arg1	CGA					100:102	CGA	100:102	CGA	100:102	The charophycean green algae (CGA or basal streptophytes) are of particular evolutionary significance because their ancestors gave rise to land plants.					
34140625	6	6	theme	evolutionary	837:848	arg1	elaboration					850:860	a late evolutionary elaboration	830:860	a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes	830:943	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	6	7	located	present	961:967	arg1	caldariorum					984:994	Mesotaenium caldariorum	972:994	Mesotaenium caldariorum	972:994	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	6	7	located	present	961:967	arg2	feature					808:814	a feature	806:814	a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes	806:943	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	6	7	located	present	961:967	arg2	fucosylation					792:803	XyG fucosylation	788:803	XyG fucosylation	788:803	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	3	8	from	absent	463:468	arg1	CGA					473:475	CGA	473:475	CGA	473:475	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	2	9	theme	cell	275:278	arg1	walls					280:284	their cell walls	269:284	their cell walls	269:284	One outstanding feature of these algae is that their cell walls exhibit remarkable similarities to those of land plants.					
34140625	5	10	dep	machinery	603:611	arg1	produce					616:622	produce	616:622	to produce XyG	613:626	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	11	theme	uronic	729:734	arg1	XyG					752:754	uronic acid-containing XyG	729:754	uronic acid-containing XyG	729:754	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	12	from	elucidation	699:709	arg1	CGA					769:771	selected CGA	760:771	selected CGA	760:771	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	12	from	elucidation	699:709	arg1	range					663:667	a range	661:667	a range of CGA	661:674	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	3	13	from	CGA	473:475	arg1	absent					463:468	absent	463:468	absent	463:468	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	3	14	theme	walls	404:408	arg1	Xyloglucan					343:352	Xyloglucan	343:352	Xyloglucan (XyG)	343:358	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	3	14	theme	walls	404:408	arg1	component					382:390	a major structural component	363:390	a major structural component of the cell walls of most land plants	363:428	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	3	15	theme	structural	371:380	arg1	Xyloglucan					343:352	Xyloglucan	343:352	Xyloglucan (XyG)	343:358	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	3	15	theme	structural	371:380	arg1	component					382:390	a major structural component	363:390	a major structural component of the cell walls of most land plants	363:428	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	1	16	theme	charophycean	74:85	arg1	algae					93:97	The charophycean green algae	70:97	The charophycean green algae (CGA or basal streptophytes)	70:126	The charophycean green algae (CGA or basal streptophytes) are of particular evolutionary significance because their ancestors gave rise to land plants.					
34140625	5	17	theme	acid-containing	736:750	arg1	XyG					752:754	uronic acid-containing XyG	729:754	uronic acid-containing XyG	729:754	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	6	18	theme	orthologs	893:901	arg1	elaboration					850:860	a late evolutionary elaboration	830:860	a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes	830:943	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	1	19	theme	green	87:91	arg1	algae					93:97	The charophycean green algae	70:97	The charophycean green algae (CGA or basal streptophytes)	70:126	The charophycean green algae (CGA or basal streptophytes) are of particular evolutionary significance because their ancestors gave rise to land plants.					
34140625	0	20	theme	Ancient	0:6	arg1	origin					8:13	Ancient origin	0:13	Ancient origin of fucosylated xyloglucan in charophycean green algae.	0:68	Ancient origin of fucosylated xyloglucan in charophycean green algae.					
34140625	6	21	theme	late	832:835	arg1	elaboration					850:860	a late evolutionary elaboration	830:860	a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes	830:943	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	2	22	theme	remarkable	294:303	arg1	similarities					305:316	remarkable similarities	294:316	remarkable similarities to those of land plants	294:340	One outstanding feature of these algae is that their cell walls exhibit remarkable similarities to those of land plants.					
34140625	3	23	theme	cell	399:402	arg1	walls					404:408	the cell walls	395:408	the cell walls of most land plants	395:428	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	0	24	theme	fucosylated	18:28	arg1	xyloglucan					30:39	fucosylated xyloglucan	18:39	fucosylated xyloglucan	18:39	Ancient origin of fucosylated xyloglucan in charophycean green algae.					
34140625	5	25	from	identification	560:573	arg1	CGA					769:771	selected CGA	760:771	selected CGA	760:771	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	25	from	identification	560:573	arg1	range					663:667	a range	661:667	a range of CGA	661:674	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	3	26	theme	plants	423:428	arg1	walls					404:408	the cell walls	395:408	the cell walls of most land plants	395:428	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	6	27	attach	present	961:967	arg2	fucosylation					792:803	XyG fucosylation	788:803	XyG fucosylation	788:803	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	6	27	attach	present	961:967	arg2	feature					808:814	a feature	806:814	a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes	806:943	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	6	27	attach	present	961:967	arg1	caldariorum					984:994	Mesotaenium caldariorum	972:994	Mesotaenium caldariorum	972:994	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	1	28	theme	evolutionary	146:157	arg1	significance					159:170	particular evolutionary significance	135:170	particular evolutionary significance	135:170	The charophycean green algae (CGA or basal streptophytes) are of particular evolutionary significance because their ancestors gave rise to land plants.					
34140625	5	29	theme	XyG	714:716	arg1	identification					560:573	the identification	556:573	the identification of orthologs of the genetic machinery to produce XyG	556:626	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	29	theme	XyG	714:716	arg1	identification					636:649	the identification	632:649	b) the identification of XyG in a range of CGA	629:674	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	29	theme	XyG	714:716	arg1	elucidation					699:709	the structural elucidation	684:709	c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA	681:771	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	30	theme	CGA	672:674	arg1	range					663:667	a range	661:667	a range of CGA	661:674	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	2	31	theme	outstanding	226:236	arg1	feature					238:244	One outstanding feature	222:244	One outstanding feature of these algae	222:259	One outstanding feature of these algae is that their cell walls exhibit remarkable similarities to those of land plants.					
34140625	6	32	theme	biosynthetic	924:935	arg1	enzymes					937:943	the corresponding biosynthetic enzymes	906:943	the corresponding biosynthetic enzymes	906:943	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	3	33	theme	most	413:416	arg1	plants					423:428	most land plants	413:428	most land plants	413:428	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	0	34	theme	xyloglucan	30:39	arg1	origin					8:13	Ancient origin	0:13	Ancient origin of fucosylated xyloglucan in charophycean green algae.	0:68	Ancient origin of fucosylated xyloglucan in charophycean green algae.					
34140625	5	35	dep	identification	560:573	arg1	a					553:553	a	553:553	a	553:553	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	36	theme	structural	688:697	arg1	elucidation					699:709	the structural elucidation	684:709	c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA	681:771	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	6	37	from	caldariorum	984:994	arg1	present					961:967	present	961:967	present	961:967	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	1	38	theme	land	209:212	arg1	plants					214:219	land plants	209:219	land plants	209:219	The charophycean green algae (CGA or basal streptophytes) are of particular evolutionary significance because their ancestors gave rise to land plants.					
34140625	0	39	theme	green	57:61	arg1	algae					63:67	charophycean green algae	44:67	charophycean green algae	44:67	Ancient origin of fucosylated xyloglucan in charophycean green algae.					
34140625	5	40	theme	genetic	595:601	arg1	machinery					603:611	the genetic machinery	591:611	the genetic machinery to produce XyG	591:626	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	41	from	identification	636:649	arg1	CGA					769:771	selected CGA	760:771	selected CGA	760:771	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	41	from	identification	636:649	arg1	range					663:667	a range	661:667	a range of CGA	661:674	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	2	42	theme	land	330:333	arg1	plants					335:340	land plants	330:340	land plants	330:340	One outstanding feature of these algae is that their cell walls exhibit remarkable similarities to those of land plants.					
34140625	0	43	theme	charophycean	44:55	arg1	algae					63:67	charophycean green algae	44:67	charophycean green algae	44:67	Ancient origin of fucosylated xyloglucan in charophycean green algae.					
34140625	5	44	dep	elucidation	699:709	arg1	c					681:681	c	681:681	c	681:681	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	5	45	theme	machinery	603:611	arg1	orthologs					578:586	orthologs	578:586	orthologs of the genetic machinery to produce XyG	578:626	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	0	46	gly	fucosylated	18:28	arg1	xyloglucan					30:39	fucosylated xyloglucan	18:39	fucosylated xyloglucan	18:39	Ancient origin of fucosylated xyloglucan in charophycean green algae.					
34140625	1	47	theme	basal	107:111	arg1	streptophytes					113:125	basal streptophytes	107:125	basal streptophytes	107:125	The charophycean green algae (CGA or basal streptophytes) are of particular evolutionary significance because their ancestors gave rise to land plants.					
34140625	2	48	theme	algae	255:259	arg1	feature					238:244	One outstanding feature	222:244	One outstanding feature of these algae	222:259	One outstanding feature of these algae is that their cell walls exhibit remarkable similarities to those of land plants.					
34140625	5	49	dep	identification	636:649	arg1	b					629:629	b	629:629	b	629:629	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	6	50	theme	structure	879:887	arg1	elaboration					850:860	a late evolutionary elaboration	830:860	a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes	830:943	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	3	51	theme	land	418:421	arg1	plants					423:428	most land plants	413:428	most land plants	413:428	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	6	52	theme	corresponding	910:922	arg1	enzymes					937:943	the corresponding biosynthetic enzymes	906:943	the corresponding biosynthetic enzymes	906:943	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	6	53	theme	XyG	875:877	arg1	structure					879:887	the basic XyG structure	865:887	the basic XyG structure	865:887	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	3	54	theme	major	365:369	arg1	Xyloglucan					343:352	Xyloglucan	343:352	Xyloglucan (XyG)	343:358	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	3	54	theme	major	365:369	arg1	component					382:390	a major structural component	363:390	a major structural component of the cell walls of most land plants	363:428	Xyloglucan (XyG) is a major structural component of the cell walls of most land plants and was originally thought to be absent in CGA.					
34140625	6	55	theme	XyG	788:790	arg1	fucosylation					792:803	XyG fucosylation	788:803	XyG fucosylation	788:803	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	6	55	theme	XyG	788:790	arg1	feature					808:814	a feature	806:814	a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes	806:943	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
34140625	5	56	theme	selected	760:767	arg1	CGA					769:771	selected CGA	760:771	selected CGA	760:771	This is based on a) the identification of orthologs of the genetic machinery to produce XyG, b) the identification of XyG in a range of CGA and, c) the structural elucidation of XyG, including uronic acid-containing XyG, in selected CGA.					
34140625	6	57	theme	basic	869:873	arg1	structure					879:887	the basic XyG structure	865:887	the basic XyG structure	865:887	Most notably, XyG fucosylation, a feature considered as a late evolutionary elaboration of the basic XyG structure and orthologs to the corresponding biosynthetic enzymes are shown to be present in Mesotaenium caldariorum.					
32420732	2	0	theme	backbone	481:488	arg1	exchange					497:504	backbone proton exchange	481:504	backbone proton exchange	481:504	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	4	1	theme	charged	883:889	arg1	glycans					891:897	negatively charged glycans	872:897	negatively charged glycans	872:897	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	1	2	theme	structures	178:187	arg1	Characterization					141:156	Characterization	141:156	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S)	141:231	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	3	3	theme	samples	732:738	arg1	comparability					668:680	analytical comparability	657:680	analytical comparability of different batches of drug substance as well as samples with modulated glycans	657:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	5	4	theme	I2S	1105:1107	arg1	uptake					1109:1114	I2S uptake	1105:1114	I2S uptake into cells	1105:1125	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	1	5	from	structures	178:187	arg1	idursulfase					192:202	idursulfase	192:202	idursulfase (iduronate-2-sulfatase, I2S)	192:231	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	1	6	theme	hydrogen-deuterium	274:291	arg1	HDX-MS					321:326	HDX-MS	321:326	HDX-MS	321:326	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	1	6	theme	hydrogen-deuterium	274:291	arg1	spectrometry					307:318	hydrogen-deuterium exchange mass spectrometry	274:318	hydrogen-deuterium exchange mass spectrometry (HDX-MS)	274:327	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	0	7	theme	Hydrogen-Deuterium	94:111	arg1	Spectrometry					127:138	Hydrogen-Deuterium Exchange Mass Spectrometry	94:138	Hydrogen-Deuterium Exchange Mass Spectrometry	94:138	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.					
32420732	0	8	from	Impact	0:5	arg1	Comparability					31:43	the Comparability	27:43	the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry	27:138	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.					
32420732	4	9	theme	sialic	841:846	arg1	removal					821:827	enzymatic removal	811:827	enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans	811:897	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	5	10	theme	HDX	984:986	arg1	changes					988:994	synergistic HDX changes	972:994	synergistic HDX changes	972:994	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	1	11	theme	exchange	293:300	arg1	HDX-MS					321:326	HDX-MS	321:326	HDX-MS	321:326	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	1	11	theme	exchange	293:300	arg1	spectrometry					307:318	hydrogen-deuterium exchange mass spectrometry	274:318	hydrogen-deuterium exchange mass spectrometry (HDX-MS)	274:327	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	2	12	from	impact	454:459	arg1	exchange					497:504	backbone proton exchange	481:504	backbone proton exchange	481:504	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	1	13	theme	mass	302:305	arg1	HDX-MS					321:326	HDX-MS	321:326	HDX-MS	321:326	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	1	13	theme	mass	302:305	arg1	spectrometry					307:318	hydrogen-deuterium exchange mass spectrometry	274:318	hydrogen-deuterium exchange mass spectrometry (HDX-MS)	274:327	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	0	14	theme	Mass	122:125	arg1	Spectrometry					127:138	Hydrogen-Deuterium Exchange Mass Spectrometry	94:138	Hydrogen-Deuterium Exchange Mass Spectrometry	94:138	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.					
32420732	2	15	used	used	436:439	arg2	method					334:339	The method	330:339	The method	330:339	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	4	16	theme	phosphate	852:860	arg1	groups					862:867	terminal sialic and phosphate groups	832:867	groups	862:867	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	2	17	theme	glycosylation	402:414	arg1	sites					416:420	the eight glycosylation sites	392:420	the eight glycosylation sites	392:420	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	0	18	theme	Exchange	113:120	arg1	Spectrometry					127:138	Hydrogen-Deuterium Exchange Mass Spectrometry	94:138	Hydrogen-Deuterium Exchange Mass Spectrometry	94:138	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.					
32420732	0	19	from	Comparability	31:43	arg1	Idursulfase					79:89	Idursulfase	79:89	Idursulfase	79:89	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.					
32420732	2	20	gly	glycosylation	402:414	arg2	sites					416:420	the eight glycosylation sites	392:420	the eight glycosylation sites	392:420	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	2	20	gly	glycosylation	402:414	arg2	eight					396:400	eight	396:400	eight	396:400	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	1	21	from	Characterization	141:156	arg1	idursulfase					192:202	idursulfase	192:202	idursulfase (iduronate-2-sulfatase, I2S)	192:231	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	4	22	theme	HDX	783:785	arg1	profile					787:793	the HDX profile	779:793	the HDX profile	779:793	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	3	23	theme	spectra	563:569	arg1	method					582:587	a well-used biophysical spectra comparison method	539:587	a well-used biophysical spectra comparison method (similarity scoring)	539:608	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	23	theme	spectra	563:569	arg1	scoring					601:607	similarity scoring	590:607	similarity scoring	590:607	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	24	with	batches	695:701	arg1	glycans					755:761	modulated glycans	745:761	modulated glycans	745:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	1	25	theme	spectrometry	307:318	arg1	use					267:269	the use	263:269	the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS)	263:327	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	4	26	theme	groups	862:867	arg1	removal					821:827	enzymatic removal	811:827	enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans	811:897	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	3	27	theme	comparison	571:580	arg1	method					582:587	a well-used biophysical spectra comparison method	539:587	a well-used biophysical spectra comparison method (similarity scoring)	539:608	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	27	theme	comparison	571:580	arg1	scoring					601:607	similarity scoring	590:607	similarity scoring	590:607	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	5	28	theme	N255	1024:1027	arg1	sites					1043:1047	the N221 and N255 glycosylation sites	1011:1047	sites	1043:1047	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	3	29	theme	well-used	541:549	arg1	method					582:587	a well-used biophysical spectra comparison method	539:587	a well-used biophysical spectra comparison method (similarity scoring)	539:608	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	29	theme	well-used	541:549	arg1	scoring					601:607	similarity scoring	590:607	similarity scoring	590:607	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	5	30	theme	mannose-6-phosphate	1064:1082	arg1	motifs					1084:1089	mannose-6-phosphate motifs	1064:1089	mannose-6-phosphate motifs important for I2S uptake into cells	1064:1125	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	0	31	theme	Glycosylation	10:22	arg1	Impact					0:5	Impact	0:5	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.	0:139	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.					
32420732	3	32	theme	modulated	745:753	arg1	glycans					755:761	modulated glycans	745:761	modulated glycans	745:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	0	33	from	Idursulfase	79:89	arg1	Comparability					31:43	the Comparability	27:43	the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry	27:138	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.					
32420732	3	34	theme	biophysical	551:561	arg1	method					582:587	a well-used biophysical spectra comparison method	539:587	a well-used biophysical spectra comparison method (similarity scoring)	539:608	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	34	theme	biophysical	551:561	arg1	scoring					601:607	similarity scoring	590:607	similarity scoring	590:607	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	35	theme	acceptance	633:642	arg1	criteria					644:651	quantitative acceptance criteria	620:651	quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans	620:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	2	36	theme	glycosylation	464:476	arg1	impact					454:459	the impact	450:459	the impact of glycosylation on backbone proton exchange	450:504	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	3	37	theme	analytical	657:666	arg1	comparability					668:680	analytical comparability	657:680	analytical comparability of different batches of drug substance as well as samples with modulated glycans	657:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	38	theme	similarity	590:599	arg1	method					582:587	a well-used biophysical spectra comparison method	539:587	a well-used biophysical spectra comparison method (similarity scoring)	539:608	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	38	theme	similarity	590:599	arg1	scoring					601:607	similarity scoring	590:607	similarity scoring	590:607	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	4	39	from	Differences	764:774	arg1	profile					787:793	the HDX profile	779:793	the HDX profile	779:793	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	0	40	from	Structures	65:74	arg1	Idursulfase					79:89	Idursulfase	79:89	Idursulfase	79:89	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.					
32420732	2	41	theme	sites	416:420	arg1	sites					416:420	the eight glycosylation sites	392:420	the eight glycosylation sites	392:420	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	2	41	theme	sites	416:420	arg1	seven					383:387	seven	383:387	seven	383:387	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	5	42	gly	glycosylation	1029:1041	arg2	sites					1043:1047	the N221 and N255 glycosylation sites	1011:1047	sites	1043:1047	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	2	43	theme	proton	490:495	arg1	exchange					497:504	backbone proton exchange	481:504	backbone proton exchange	481:504	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	1	44	dep	idursulfase	192:202	arg1	iduronate-2-sulfatase					205:225	iduronate-2-sulfatase	205:225	iduronate-2-sulfatase	205:225	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	1	44	dep	idursulfase	192:202	arg1	I2S					228:230	I2S	228:230	I2S	228:230	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	2	45	theme	sequence	354:361	arg1	coverage					363:370	97% sequence coverage	350:370	97% sequence coverage	350:370	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	3	46	theme	drug	706:709	arg1	substance					711:719	drug substance	706:719	drug substance	706:719	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	47	theme	different	685:693	arg1	batches					695:701	different batches	685:701	different batches of drug substance as well as samples with modulated glycans	685:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	4	48	theme	terminal	832:839	arg1	sialic					841:846	terminal sialic and phosphate groups	832:867	sialic	841:846	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	2	49	theme	%	352:352	arg1	coverage					363:370	97% sequence coverage	350:370	97% sequence coverage	350:370	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	5	50	contain	contain	1056:1062	arg2	motifs					1084:1089	mannose-6-phosphate motifs	1064:1089	mannose-6-phosphate motifs important for I2S uptake into cells	1064:1125	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	5	50	contain	contain	1056:1062	arg1	changes					988:994	synergistic HDX changes	972:994	synergistic HDX changes	972:994	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	3	51	theme	batches	695:701	arg1	comparability					668:680	analytical comparability	657:680	analytical comparability of different batches of drug substance as well as samples with modulated glycans	657:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	4	52	from	removal	821:827	arg1	glycans					891:897	negatively charged glycans	872:897	negatively charged glycans	872:897	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	2	53	theme	97	350:351	arg1	%					352:352	%	352:352	%	352:352	The method has over 97% sequence coverage, including seven of the eight glycosylation sites, and has been used to study the impact of glycosylation on backbone proton exchange.					
32420732	5	54	theme	synergistic	972:982	arg1	changes					988:994	synergistic HDX changes	972:994	synergistic HDX changes	972:994	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	4	55	attach	removal	821:827	arg2	sialic					841:846	terminal sialic and phosphate groups	832:867	sialic	841:846	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	4	55	attach	removal	821:827	arg3	glycans					891:897	negatively charged glycans	872:897	negatively charged glycans	872:897	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	4	55	attach	removal	821:827	arg2	groups					862:867	terminal sialic and phosphate groups	832:867	groups	862:867	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	5	56	theme	important	1091:1099	arg1	motifs					1084:1089	mannose-6-phosphate motifs	1064:1089	mannose-6-phosphate motifs important for I2S uptake into cells	1064:1125	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	3	57	theme	quantitative	620:631	arg1	criteria					644:651	quantitative acceptance criteria	620:651	quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans	620:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	58	with	samples	732:738	arg1	glycans					755:761	modulated glycans	745:761	modulated glycans	745:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	5	59	theme	crystal	937:943	arg1	structure					945:953	the crystal structure	933:953	the crystal structure	933:953	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	0	60	theme	Structures	65:74	arg1	Comparability					31:43	the Comparability	27:43	the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry	27:138	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.					
32420732	4	61	theme	enzymatic	811:819	arg1	removal					821:827	enzymatic removal	811:827	enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans	811:897	Differences in the HDX profile were induced by enzymatic removal of terminal sialic and phosphate groups on negatively charged glycans.					
32420732	0	62	theme	Higher-Order	52:63	arg1	Structures					65:74	the Higher-Order Structures	48:74	the Higher-Order Structures in Idursulfase	48:89	Impact of Glycosylation on the Comparability of the Higher-Order Structures in Idursulfase by Hydrogen-Deuterium Exchange Mass Spectrometry.					
32420732	3	63	theme	substance	711:719	arg1	samples					732:738	samples	732:738	different batches of drug substance as well as samples with modulated glycans	685:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	3	63	theme	substance	711:719	arg1	batches					695:701	different batches	685:701	different batches of drug substance as well as samples with modulated glycans	685:761	In addition, the method adapted a well-used biophysical spectra comparison method (similarity scoring) to define quantitative acceptance criteria for analytical comparability of different batches of drug substance as well as samples with modulated glycans.					
32420732	5	64	theme	glycosylation	1029:1041	arg1	sites					1043:1047	the N221 and N255 glycosylation sites	1011:1047	sites	1043:1047	These differences were mapped to the crystal structure and demonstrated synergistic HDX changes focused around the N221 and N255 glycosylation sites, which contain mannose-6-phosphate motifs important for I2S uptake into cells.					
32420732	1	65	from	idursulfase	192:202	arg1	Characterization					141:156	Characterization	141:156	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S)	141:231	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32420732	1	66	theme	higher-order	165:176	arg1	structures					178:187	the higher-order structures	161:187	the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S)	161:231	Characterization of the higher-order structures in idursulfase (iduronate-2-sulfatase, I2S) has been accomplished through the use of hydrogen-deuterium exchange mass spectrometry (HDX-MS).					
32531635	0	0	theme	upper	81:85	arg1	tract					97:101	the upper digestive tract	77:101	the upper digestive tract	77:101	Heat treatment of β-lactoglobulin affects its digestion and translocation in the upper digestive tract.					
32531635	7	1	theme	particular	1033:1042	arg1	digestibility					1052:1064	particular gastric digestibility	1033:1064	particular gastric digestibility	1033:1064	Our study indicates that structural modifications after different heat-treatments of β-lactoglobulin increase in particular gastric digestibility and the translocation efficiency across intestinal epithelial cells.					
32531635	4	2	theme	immune	603:608	arg1	cells					610:614	immune cells	603:614	immune cells	603:614	Additionally, protein was investigated for its translocation over the intestinal epithelial barrier, which would bring them in contact with immune cells.					
32531635	4	3	theme	epithelial	544:553	arg1	barrier					555:561	the intestinal epithelial barrier	529:561	the intestinal epithelial barrier	529:561	Additionally, protein was investigated for its translocation over the intestinal epithelial barrier, which would bring them in contact with immune cells.					
32531635	5	4	theme	protein	673:679	arg1	structure					681:689	the protein structure	669:689	the protein structure	669:689	The extent of gastric digestibility was higher when the protein structure was more modified, while the influence of glycation with lactose was limited.					
32531635	1	5	from	operation	146:154	arg1	processing					163:172	the processing	159:172	the processing of β-lactoglobulin containing products	159:211	Heat treatment is a commonly applied unit operation in the processing of β-lactoglobulin containing products.					
32531635	4	6	theme	intestinal	533:542	arg1	barrier					555:561	the intestinal epithelial barrier	529:561	the intestinal epithelial barrier	529:561	Additionally, protein was investigated for its translocation over the intestinal epithelial barrier, which would bring them in contact with immune cells.					
32531635	4	7	with	contact	590:596	arg1	cells					610:614	immune cells	603:614	immune cells	603:614	Additionally, protein was investigated for its translocation over the intestinal epithelial barrier, which would bring them in contact with immune cells.					
32531635	0	8	theme	digestive	87:95	arg1	tract					97:101	the upper digestive tract	77:101	the upper digestive tract	77:101	Heat treatment of β-lactoglobulin affects its digestion and translocation in the upper digestive tract.					
32531635	7	9	theme	β-lactoglobulin	1005:1019	arg1	heat-treatments					986:1000	different heat-treatments	976:1000	different heat-treatments of β-lactoglobulin	976:1019	Our study indicates that structural modifications after different heat-treatments of β-lactoglobulin increase in particular gastric digestibility and the translocation efficiency across intestinal epithelial cells.					
32531635	0	10	from	digestion	46:54	arg1	tract					97:101	the upper digestive tract	77:101	the upper digestive tract	77:101	Heat treatment of β-lactoglobulin affects its digestion and translocation in the upper digestive tract.					
32531635	5	11	theme	digestibility	639:651	arg1	higher					657:662	higher	657:662	higher	657:662	The extent of gastric digestibility was higher when the protein structure was more modified, while the influence of glycation with lactose was limited.					
32531635	5	11	theme	digestibility	639:651	arg1	extent					621:626	The extent	617:626	The extent of gastric digestibility	617:651	The extent of gastric digestibility was higher when the protein structure was more modified, while the influence of glycation with lactose was limited.					
32531635	5	12	theme	gastric	631:637	arg1	digestibility					639:651	gastric digestibility	631:651	gastric digestibility	631:651	The extent of gastric digestibility was higher when the protein structure was more modified, while the influence of glycation with lactose was limited.					
32531635	3	13	theme	protocol	453:460	arg1	version					411:417	a modified version	400:417	a modified version of the current consensus INFOGEST protocol	400:460	We describe how various heat-treatments of β-lactoglobulin change the digestibility using a modified version of the current consensus INFOGEST protocol.					
32531635	3	14	theme	β-lactoglobulin	353:367	arg1	heat-treatments					334:348	various heat-treatments	326:348	various heat-treatments of β-lactoglobulin	326:367	We describe how various heat-treatments of β-lactoglobulin change the digestibility using a modified version of the current consensus INFOGEST protocol.					
32531635	6	15	theme	cell	816:819	arg1	monolayers					821:830	Caco-2 cell monolayers	809:830	Caco-2 cell monolayers	809:830	Translocation studies of protein across Caco-2 cell monolayers showed a lower translocation rate of protein heated in solution compared to the others.					
32531635	0	16	theme	Heat	0:3	arg1	treatment					5:13	Heat treatment	0:13	Heat treatment of β-lactoglobulin	0:32	Heat treatment of β-lactoglobulin affects its digestion and translocation in the upper digestive tract.					
32531635	6	17	theme	Caco-2	809:814	arg1	monolayers					821:830	Caco-2 cell monolayers	809:830	Caco-2 cell monolayers	809:830	Translocation studies of protein across Caco-2 cell monolayers showed a lower translocation rate of protein heated in solution compared to the others.					
32531635	3	18	theme	consensus	434:442	arg1	protocol					453:460	the current consensus INFOGEST protocol	422:460	the current consensus INFOGEST protocol	422:460	We describe how various heat-treatments of β-lactoglobulin change the digestibility using a modified version of the current consensus INFOGEST protocol.					
32531635	3	19	theme	modified	402:409	arg1	version					411:417	a modified version	400:417	a modified version of the current consensus INFOGEST protocol	400:460	We describe how various heat-treatments of β-lactoglobulin change the digestibility using a modified version of the current consensus INFOGEST protocol.					
32531635	7	20	theme	structural	945:954	arg1	modifications					956:968	structural modifications	945:968	structural modifications after different heat-treatments of β-lactoglobulin	945:1019	Our study indicates that structural modifications after different heat-treatments of β-lactoglobulin increase in particular gastric digestibility and the translocation efficiency across intestinal epithelial cells.					
32531635	1	21	theme	β-lactoglobulin	177:191	arg1	products					204:211	β-lactoglobulin containing products	177:211	β-lactoglobulin containing products	177:211	Heat treatment is a commonly applied unit operation in the processing of β-lactoglobulin containing products.					
32531635	0	22	theme	β-lactoglobulin	18:32	arg1	treatment					5:13	Heat treatment	0:13	Heat treatment of β-lactoglobulin	0:32	Heat treatment of β-lactoglobulin affects its digestion and translocation in the upper digestive tract.					
32531635	6	23	theme	protein	794:800	arg1	studies					783:789	Translocation studies	769:789	Translocation studies of protein across Caco-2 cell monolayers	769:830	Translocation studies of protein across Caco-2 cell monolayers showed a lower translocation rate of protein heated in solution compared to the others.					
32531635	6	24	theme	lower	841:845	arg1	rate					861:864	a lower translocation rate	839:864	a lower translocation rate of protein heated in solution compared to the others	839:917	Translocation studies of protein across Caco-2 cell monolayers showed a lower translocation rate of protein heated in solution compared to the others.					
32531635	1	25	theme	containing	193:202	arg1	products					204:211	β-lactoglobulin containing products	177:211	β-lactoglobulin containing products	177:211	Heat treatment is a commonly applied unit operation in the processing of β-lactoglobulin containing products.					
32531635	1	26	theme	Heat	104:107	arg1	treatment					109:117	Heat treatment	104:117	Heat treatment	104:117	Heat treatment is a commonly applied unit operation in the processing of β-lactoglobulin containing products.					
32531635	1	26	theme	Heat	104:107	arg1	operation					146:154	a commonly applied unit operation	122:154	a commonly applied unit operation in the processing of β-lactoglobulin containing products	122:211	Heat treatment is a commonly applied unit operation in the processing of β-lactoglobulin containing products.					
32531635	1	27	theme	products	204:211	arg1	processing					163:172	the processing	159:172	the processing of β-lactoglobulin containing products	159:211	Heat treatment is a commonly applied unit operation in the processing of β-lactoglobulin containing products.					
32531635	3	28	theme	INFOGEST	444:451	arg1	protocol					453:460	the current consensus INFOGEST protocol	422:460	the current consensus INFOGEST protocol	422:460	We describe how various heat-treatments of β-lactoglobulin change the digestibility using a modified version of the current consensus INFOGEST protocol.					
32531635	3	29	theme	current	426:432	arg1	protocol					453:460	the current consensus INFOGEST protocol	422:460	the current consensus INFOGEST protocol	422:460	We describe how various heat-treatments of β-lactoglobulin change the digestibility using a modified version of the current consensus INFOGEST protocol.					
32531635	5	30	theme	glycation	733:741	arg1	influence					720:728	the influence	716:728	the influence of glycation with lactose	716:754	The extent of gastric digestibility was higher when the protein structure was more modified, while the influence of glycation with lactose was limited.					
32531635	7	31	theme	translocation	1074:1086	arg1	efficiency					1088:1097	the translocation efficiency	1070:1097	the translocation efficiency	1070:1097	Our study indicates that structural modifications after different heat-treatments of β-lactoglobulin increase in particular gastric digestibility and the translocation efficiency across intestinal epithelial cells.					
32531635	5	32	with	influence	720:728	arg1	lactose					748:754	lactose	748:754	lactose	748:754	The extent of gastric digestibility was higher when the protein structure was more modified, while the influence of glycation with lactose was limited.					
32531635	7	33	theme	epithelial	1117:1126	arg1	cells					1128:1132	intestinal epithelial cells	1106:1132	intestinal epithelial cells	1106:1132	Our study indicates that structural modifications after different heat-treatments of β-lactoglobulin increase in particular gastric digestibility and the translocation efficiency across intestinal epithelial cells.					
32531635	0	34	from	translocation	60:72	arg1	tract					97:101	the upper digestive tract	77:101	the upper digestive tract	77:101	Heat treatment of β-lactoglobulin affects its digestion and translocation in the upper digestive tract.					
32531635	6	35	theme	protein	869:875	arg1	rate					861:864	a lower translocation rate	839:864	a lower translocation rate of protein heated in solution compared to the others	839:917	Translocation studies of protein across Caco-2 cell monolayers showed a lower translocation rate of protein heated in solution compared to the others.					
32531635	6	36	theme	Translocation	769:781	arg1	studies					783:789	Translocation studies	769:789	Translocation studies of protein across Caco-2 cell monolayers	769:830	Translocation studies of protein across Caco-2 cell monolayers showed a lower translocation rate of protein heated in solution compared to the others.					
32531635	7	37	theme	intestinal	1106:1115	arg1	cells					1128:1132	intestinal epithelial cells	1106:1132	intestinal epithelial cells	1106:1132	Our study indicates that structural modifications after different heat-treatments of β-lactoglobulin increase in particular gastric digestibility and the translocation efficiency across intestinal epithelial cells.					
32531635	7	38	theme	gastric	1044:1050	arg1	digestibility					1052:1064	particular gastric digestibility	1033:1064	particular gastric digestibility	1033:1064	Our study indicates that structural modifications after different heat-treatments of β-lactoglobulin increase in particular gastric digestibility and the translocation efficiency across intestinal epithelial cells.					
32531635	3	39	theme	various	326:332	arg1	heat-treatments					334:348	various heat-treatments	326:348	various heat-treatments of β-lactoglobulin	326:367	We describe how various heat-treatments of β-lactoglobulin change the digestibility using a modified version of the current consensus INFOGEST protocol.					
32531635	6	40	theme	translocation	847:859	arg1	rate					861:864	a lower translocation rate	839:864	a lower translocation rate of protein heated in solution compared to the others	839:917	Translocation studies of protein across Caco-2 cell monolayers showed a lower translocation rate of protein heated in solution compared to the others.					
32531635	1	41	theme	applied	133:139	arg1	treatment					109:117	Heat treatment	104:117	Heat treatment	104:117	Heat treatment is a commonly applied unit operation in the processing of β-lactoglobulin containing products.					
32531635	1	41	theme	applied	133:139	arg1	operation					146:154	a commonly applied unit operation	122:154	a commonly applied unit operation in the processing of β-lactoglobulin containing products	122:211	Heat treatment is a commonly applied unit operation in the processing of β-lactoglobulin containing products.					
32531635	7	42	theme	different	976:984	arg1	heat-treatments					986:1000	different heat-treatments	976:1000	different heat-treatments of β-lactoglobulin	976:1019	Our study indicates that structural modifications after different heat-treatments of β-lactoglobulin increase in particular gastric digestibility and the translocation efficiency across intestinal epithelial cells.					
32531635	1	43	theme	unit	141:144	arg1	treatment					109:117	Heat treatment	104:117	Heat treatment	104:117	Heat treatment is a commonly applied unit operation in the processing of β-lactoglobulin containing products.					
32531635	1	43	theme	unit	141:144	arg1	operation					146:154	a commonly applied unit operation	122:154	a commonly applied unit operation in the processing of β-lactoglobulin containing products	122:211	Heat treatment is a commonly applied unit operation in the processing of β-lactoglobulin containing products.					
33822377	7	0	theme	index	1231:1235	arg1	value					1241:1245	the flow index (n) value	1222:1245	the flow index (n) value	1222:1245	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	9	1	theme	application	1533:1543	arg1	prospects					1545:1553	potential industrial application prospects	1512:1553	potential industrial application prospects	1512:1553	Therefore, this research provided an effective method for improving the functional characteristics of SPI and had potential industrial application prospects.					
33822377	10	2	theme	further	1818:1824	arg1	development					1826:1836	further development	1818:1836	further development	1818:1836	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	3	theme	soybean	1857:1863	arg1	protein					1865:1871	soybean protein	1857:1871	soybean protein	1857:1871	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	0	4	theme	physicochemical	73:87	arg1	properties					89:98	physicochemical properties	73:98	physicochemical properties	73:98	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.					
33822377	10	5	theme	new	1801:1803	arg1	idea					1805:1808	a new idea	1799:1808	a new idea for the further development and utilization of soybean protein	1799:1871	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	1	6	theme	different	159:167	arg1	treatments					180:189	different sequential treatments	159:189	different sequential treatments of dextran glycation and succinic anhydride acylation	159:243	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	1	7	theme	physicochemical	266:280	arg1	properties					282:291	physicochemical properties	266:291	physicochemical properties	266:291	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	0	8	theme	soy	103:105	arg1	protein					107:113	soy protein	103:113	soy protein	103:113	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.					
33822377	0	9	from	Effects	0:6	arg1	characteristics					53:67	structural characteristics	42:67	structural characteristics	42:67	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.					
33822377	0	9	from	Effects	0:6	arg1	properties					89:98	physicochemical properties	73:98	physicochemical properties	73:98	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.					
33822377	10	10	theme	isolate	1622:1628	arg1	acylation					1593:1601	acylation	1593:1601	acylation	1593:1601	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	10	theme	isolate	1622:1628	arg1	Glycation					1579:1587	Glycation	1579:1587	Glycation	1579:1587	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	11	theme	protein	1675:1681	arg1	method					1665:1670	the chemical modification method	1639:1670	the chemical modification method of protein	1639:1681	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	12	theme	functional	1697:1706	arg1	properties					1708:1717	the functional properties	1693:1717	the functional properties of soybean protein	1693:1736	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	13	theme	modification	1652:1663	arg1	method					1665:1670	the chemical modification method	1639:1670	the chemical modification method of protein	1639:1681	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	7	14	theme	SPI	1168:1170	arg1	solution					1172:1179	the SPI solution	1164:1179	the SPI solution	1164:1179	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	5	15	theme	77 ±	917:920	arg1	pH = 7					909:914	pH = 7	909:914	pH = 7 (77 ± 3.97%)	909:927	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	5	15	theme	77 ±	917:920	arg1	%					926:926	77 ± 3.97%	917:926	77 ± 3.97%	917:926	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	3	16	dep	structure	682:690	arg1	the					678:680	the	678:680	the	678:680	The results show that the two treatments significantly improved the structure and functional characteristics of the SPI.					
33822377	1	17	from	effects	148:154	arg1	structure					252:260	structure	252:260	structure	252:260	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	1	17	from	effects	148:154	arg1	properties					282:291	physicochemical properties	266:291	physicochemical properties	266:291	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	7	18	theme	conjugate	1286:1294	arg1	system					1296:1301	the conjugate system	1282:1301	the conjugate system	1282:1301	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	7	19	theme	flow	1226:1229	arg1	value					1241:1245	the flow index (n) value	1222:1245	the flow index (n) value	1222:1245	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	1	20	theme	sequential	169:178	arg1	treatments					180:189	different sequential treatments	159:189	different sequential treatments of dextran glycation and succinic anhydride acylation	159:243	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	0	21	theme	protein	107:113	arg1	characteristics					53:67	structural characteristics	42:67	structural characteristics	42:67	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.					
33822377	0	21	theme	protein	107:113	arg1	properties					89:98	physicochemical properties	73:98	physicochemical properties	73:98	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.					
33822377	2	22	theme	endogenous	423:432	arg1	spectroscopy					447:458	endogenous fluorescence spectroscopy	423:458	endogenous fluorescence spectroscopy	423:458	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	7	23	theme	viscosity	1135:1143	arg1	k					1158:1158	k	1158:1158	k	1158:1158	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	7	23	theme	viscosity	1135:1143	arg1	coefficient					1145:1155	the viscosity coefficient	1131:1155	the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone	1131:1215	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	4	24	theme	important	767:775	arg1	effect					777:782	an important effect	764:782	an important effect	764:782	The order of the methods had an important effect on the SPI.					
33822377	6	25	theme	emulsion	1029:1036	arg1	highest					1091:1097	highest	1091:1097	highest	1091:1097	Emulsification, emulsion stability, foaming, and foam stability were also the highest.					
33822377	6	25	theme	emulsion	1029:1036	arg1	Emulsification					1013:1026	Emulsification	1013:1026	Emulsification	1013:1026	Emulsification, emulsion stability, foaming, and foam stability were also the highest.					
33822377	6	25	theme	emulsion	1029:1036	arg1	stability					1038:1046	emulsion stability	1029:1046	emulsion stability	1029:1046	Emulsification, emulsion stability, foaming, and foam stability were also the highest.					
33822377	6	25	theme	emulsion	1029:1036	arg1	foaming					1049:1055	foaming	1049:1055	foaming	1049:1055	Emulsification, emulsion stability, foaming, and foam stability were also the highest.					
33822377	6	25	theme	emulsion	1029:1036	arg1	stability					1067:1075	foam stability	1062:1075	foam stability	1062:1075	Emulsification, emulsion stability, foaming, and foam stability were also the highest.					
33822377	1	26	theme	treatments	180:189	arg1	effects					148:154	the effects	144:154	the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI)	144:320	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	3	27	theme	SPI	730:732	arg1	characteristics					707:721	functional characteristics	696:721	functional characteristics	696:721	The results show that the two treatments significantly improved the structure and functional characteristics of the SPI.					
33822377	3	27	theme	SPI	730:732	arg1	structure					682:690	structure	682:690	structure	682:690	The results show that the two treatments significantly improved the structure and functional characteristics of the SPI.					
33822377	9	28	theme	effective	1435:1443	arg1	method					1445:1450	an effective method	1432:1450	an effective method for improving the functional characteristics of SPI	1432:1502	Therefore, this research provided an effective method for improving the functional characteristics of SPI and had potential industrial application prospects.					
33822377	5	29	theme	highest	887:893	arg1	solubility					895:904	the highest solubility	883:904	the highest solubility at pH = 7 (77 ± 3.97%)	883:927	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	10	30	dep	development	1826:1836	arg1	the					1814:1816	the	1814:1816	the	1814:1816	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	5	31	theme	free	840:843	arg1	content					849:855	the highest free -SH content	828:855	the highest free -SH content (3.09 ± 0.09 µmol/g)	828:876	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	5	31	theme	free	840:843	arg1	0.09 µmol/g					865:875	3.09 ± 0.09 µmol/g	858:875	3.09 ± 0.09 µmol/g	858:875	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	5	32	theme	lowest	800:805	arg1	H0					807:808	The lowest H0	796:808	The lowest H0 (231.76 ± 11.92)	796:825	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	5	32	theme	lowest	800:805	arg1	11.92					820:824	231.76 ± 11.92	811:824	231.76 ± 11.92	811:824	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	1	33	theme	soy	296:298	arg1	isolate					308:314	soy protein isolate	296:314	soy protein isolate (SPI)	296:320	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	1	33	theme	soy	296:298	arg1	SPI					317:319	SPI	317:319	SPI	317:319	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	5	34	theme	highest	832:838	arg1	content					849:855	the highest free -SH content	828:855	the highest free -SH content (3.09 ± 0.09 µmol/g)	828:876	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	5	34	theme	highest	832:838	arg1	0.09 µmol/g					865:875	3.09 ± 0.09 µmol/g	858:875	3.09 ± 0.09 µmol/g	858:875	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	1	35	theme	dextran	194:200	arg1	glycation					202:210	dextran glycation	194:210	dextran glycation	194:210	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	2	36	theme	surface	461:467	arg1	hydrophobicity					469:482	surface hydrophobicity	461:482	surface hydrophobicity (H0 )	461:488	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	2	36	theme	surface	461:467	arg1	H0					485:486	H0	485:486	H0	485:486	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	1	37	theme	protein	300:306	arg1	isolate					308:314	soy protein isolate	296:314	soy protein isolate (SPI)	296:320	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	1	37	theme	protein	300:306	arg1	SPI					317:319	SPI	317:319	SPI	317:319	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	4	38	theme	methods	752:758	arg1	order					739:743	The order	735:743	The order of the methods	735:758	The order of the methods had an important effect on the SPI.					
33822377	9	39	theme	functional	1470:1479	arg1	characteristics					1481:1495	the functional characteristics	1466:1495	the functional characteristics of SPI	1466:1502	Therefore, this research provided an effective method for improving the functional characteristics of SPI and had potential industrial application prospects.					
33822377	1	40	theme	glycation	202:210	arg1	treatments					180:189	different sequential treatments	159:189	different sequential treatments of dextran glycation and succinic anhydride acylation	159:243	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	0	41	theme	glycation	11:19	arg1	Effects					0:6	Effects	0:6	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein	0:113	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.					
33822377	1	42	theme	isolate	308:314	arg1	structure					252:260	structure	252:260	structure	252:260	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	1	42	theme	isolate	308:314	arg1	properties					282:291	physicochemical properties	266:291	physicochemical properties	266:291	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	2	43	theme	rheological	550:560	arg1	properties					562:571	rheological properties	550:571	rheological properties	550:571	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	0	44	dep	characteristics	53:67	arg1	the					38:40	the	38:40	the	38:40	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.					
33822377	10	45	from	application	1751:1761	arg1	materials					1775:1783	materials	1775:1783	materials	1775:1783	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	45	from	application	1751:1761	arg1	food					1766:1769	food	1766:1769	food	1766:1769	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	2	46	theme	fluorescence	434:445	arg1	spectroscopy					447:458	endogenous fluorescence spectroscopy	423:458	endogenous fluorescence spectroscopy	423:458	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	2	47	theme	free	491:494	arg1	-SH					508:510	-SH	508:510	-SH	508:510	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	2	47	theme	free	491:494	arg1	sulfhydryl					496:505	free sulfhydryl	491:505	free sulfhydryl (-SH)	491:511	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	10	48	theme	soybean	1606:1612	arg1	isolate					1622:1628	soybean protein isolate	1606:1628	soybean protein isolate	1606:1628	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	1	49	dep	structure	252:260	arg1	the					248:250	the	248:250	the	248:250	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	7	50	theme	sum	1266:1268	arg1	G					1270:1270	the sum G'	1262:1271	the sum G' value of the conjugate system	1262:1301	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	5	51	theme	-SH	845:847	arg1	content					849:855	the highest free -SH content	828:855	the highest free -SH content (3.09 ± 0.09 µmol/g)	828:876	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	5	51	theme	-SH	845:847	arg1	0.09 µmol/g					865:875	3.09 ± 0.09 µmol/g	858:875	3.09 ± 0.09 µmol/g	858:875	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	1	52	theme	succinic	216:223	arg1	anhydride					225:233	succinic anhydride	216:233	succinic anhydride	216:233	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	0	53	theme	acylation	25:33	arg1	Effects					0:6	Effects	0:6	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein	0:113	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.					
33822377	5	54	theme	acylation	952:960	arg1	treatment					962:970	the acylation treatment	948:970	the acylation treatment	948:970	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	9	55	theme	potential	1512:1520	arg1	application					1533:1543	potential industrial application	1512:1543	potential industrial application prospects	1512:1553	Therefore, this research provided an effective method for improving the functional characteristics of SPI and had potential industrial application prospects.					
33822377	1	56	theme	anhydride	225:233	arg1	treatments					180:189	different sequential treatments	159:189	different sequential treatments of dextran glycation and succinic anhydride acylation	159:243	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	0	57	theme	structural	42:51	arg1	characteristics					53:67	structural characteristics	42:67	structural characteristics	42:67	Effects of glycation and acylation on the structural characteristics and physicochemical properties of soy protein isolate.					
33822377	2	58	theme	interfacial	526:536	arg1	properties					538:547	interfacial properties	526:547	interfacial properties	526:547	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	2	59	theme	electron	587:594	arg1	SEM					608:610	SEM	608:610	SEM	608:610	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	2	59	theme	electron	587:594	arg1	microscope					596:605	scanning electron microscope	578:605	scanning electron microscope (SEM)	578:611	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	10	60	theme	protein	1865:1871	arg1	development					1826:1836	further development	1818:1836	further development	1818:1836	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	60	theme	protein	1865:1871	arg1	utilization					1842:1852	utilization	1842:1852	utilization	1842:1852	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	7	61	theme	gel	1316:1318	arg1	time					1320:1323	gel time	1316:1323	gel time	1316:1323	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	10	62	theme	protein	1614:1620	arg1	isolate					1622:1628	soybean protein isolate	1606:1628	soybean protein isolate	1606:1628	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	3	63	theme	functional	696:705	arg1	characteristics					707:721	functional characteristics	696:721	functional characteristics	696:721	The results show that the two treatments significantly improved the structure and functional characteristics of the SPI.					
33822377	4	64	contain	had	760:762	arg2	effect					777:782	an important effect	764:782	an important effect	764:782	The order of the methods had an important effect on the SPI.					
33822377	4	64	contain	had	760:762	arg1	order					739:743	The order	735:743	The order of the methods	735:758	The order of the methods had an important effect on the SPI.					
33822377	2	65	theme	scanning	578:585	arg1	SEM					608:610	SEM	608:610	SEM	608:610	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	2	65	theme	scanning	578:585	arg1	microscope					596:605	scanning electron microscope	578:605	scanning electron microscope (SEM)	578:611	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	9	66	theme	SPI	1500:1502	arg1	characteristics					1481:1495	the functional characteristics	1466:1495	the functional characteristics of SPI	1466:1502	Therefore, this research provided an effective method for improving the functional characteristics of SPI and had potential industrial application prospects.					
33822377	7	67	theme	n	1238:1238	arg1	value					1241:1245	the flow index (n) value	1222:1245	the flow index (n) value	1222:1245	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	6	68	theme	foam	1062:1065	arg1	highest					1091:1097	highest	1091:1097	highest	1091:1097	Emulsification, emulsion stability, foaming, and foam stability were also the highest.					
33822377	6	68	theme	foam	1062:1065	arg1	Emulsification					1013:1026	Emulsification	1013:1026	Emulsification	1013:1026	Emulsification, emulsion stability, foaming, and foam stability were also the highest.					
33822377	6	68	theme	foam	1062:1065	arg1	stability					1038:1046	emulsion stability	1029:1046	emulsion stability	1029:1046	Emulsification, emulsion stability, foaming, and foam stability were also the highest.					
33822377	6	68	theme	foam	1062:1065	arg1	foaming					1049:1055	foaming	1049:1055	foaming	1049:1055	Emulsification, emulsion stability, foaming, and foam stability were also the highest.					
33822377	6	68	theme	foam	1062:1065	arg1	stability					1067:1075	foam stability	1062:1075	foam stability	1062:1075	Emulsification, emulsion stability, foaming, and foam stability were also the highest.					
33822377	10	69	dep	APPLICATION	1566:1576	arg1	widened					1739:1745	widened	1739:1745	widened its application in food and materials	1739:1783	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	69	dep	APPLICATION	1566:1576	arg1	improved					1684:1691	improved	1684:1691	improved the functional properties of soybean protein	1684:1736	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	69	dep	APPLICATION	1566:1576	arg1	provided					1790:1797	provided	1790:1797	provided a new idea for the further development and utilization of soybean protein	1790:1871	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	69	dep	APPLICATION	1566:1576	arg1	improved					1630:1637	improved	1630:1637	improved the chemical modification method of protein	1630:1681	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	10	70	theme	soybean	1722:1728	arg1	protein					1730:1736	soybean protein	1722:1736	soybean protein	1722:1736	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	5	71	from	pH = 7	909:914	arg1	content					849:855	the highest free -SH content	828:855	the highest free -SH content (3.09 ± 0.09 µmol/g)	828:876	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	5	71	from	pH = 7	909:914	arg1	0.09 µmol/g					865:875	3.09 ± 0.09 µmol/g	858:875	3.09 ± 0.09 µmol/g	858:875	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	5	71	from	pH = 7	909:914	arg1	H0					807:808	The lowest H0	796:808	The lowest H0 (231.76 ± 11.92)	796:825	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	5	71	from	pH = 7	909:914	arg1	solubility					895:904	the highest solubility	883:904	the highest solubility at pH = 7 (77 ± 3.97%)	883:927	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	5	71	from	pH = 7	909:914	arg1	11.92					820:824	231.76 ± 11.92	811:824	231.76 ± 11.92	811:824	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	7	72	theme	system	1296:1301	arg1	value					1273:1277	the sum G' value	1262:1277	the sum G' value of the conjugate system	1262:1301	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	10	73	theme	chemical	1643:1650	arg1	method					1665:1670	the chemical modification method	1639:1670	the chemical modification method of protein	1639:1681	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	2	74	theme	tested	327:332	arg1	properties					334:343	The tested properties	323:343	The tested properties	323:343	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	5	75	theme	glycation	992:1000	arg1	treatment					1002:1010	the glycation treatment	988:1010	the glycation treatment	988:1010	The lowest H0 (231.76 ± 11.92), the highest free -SH content (3.09 ± 0.09 µmol/g), and the highest solubility at pH = 7 (77 ± 3.97%) were obtained when the acylation treatment was followed by the glycation treatment.					
33822377	1	76	dep	glycation	202:210	arg1	acylation					235:243	acylation	235:243	acylation	235:243	This study examined the effects of different sequential treatments of dextran glycation and succinic anhydride acylation on the structure and physicochemical properties of soy protein isolate (SPI).					
33822377	7	77	theme	solution	1172:1179	arg1	k					1158:1158	k	1158:1158	k	1158:1158	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	7	77	theme	solution	1172:1179	arg1	coefficient					1145:1155	the viscosity coefficient	1131:1155	the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone	1131:1215	Glycation and acylation caused the viscosity coefficient (k) of the SPI solution to decrease compared with SPI alone, but the flow index (n) value increased, and the sum G' value of the conjugate system decreased as gel time increased.					
33822377	2	78	theme	infrared	400:407	arg1	spectroscopy					409:420	infrared spectroscopy	400:420	infrared spectroscopy	400:420	The tested properties included electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, surface hydrophobicity (H0 ), free sulfhydryl (-SH), solubility, interfacial properties, rheological properties, and scanning electron microscope (SEM).					
33822377	10	79	theme	PRACTICAL	1556:1564	arg1	APPLICATION					1566:1576	PRACTICAL APPLICATION	1556:1576	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.	1556:1872	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
33822377	9	80	contain	had	1508:1510	arg1	research					1414:1421	this research	1409:1421	this research	1409:1421	Therefore, this research provided an effective method for improving the functional characteristics of SPI and had potential industrial application prospects.					
33822377	9	80	contain	had	1508:1510	arg2	prospects					1545:1553	potential industrial application prospects	1512:1553	potential industrial application prospects	1512:1553	Therefore, this research provided an effective method for improving the functional characteristics of SPI and had potential industrial application prospects.					
33822377	9	81	theme	industrial	1522:1531	arg1	application					1533:1543	potential industrial application	1512:1543	potential industrial application prospects	1512:1553	Therefore, this research provided an effective method for improving the functional characteristics of SPI and had potential industrial application prospects.					
33822377	10	82	theme	protein	1730:1736	arg1	properties					1708:1717	the functional properties	1693:1717	the functional properties of soybean protein	1693:1736	PRACTICAL APPLICATION: Glycation and acylation of soybean protein isolate improved the chemical modification method of protein, improved the functional properties of soybean protein, widened its application in food and materials, and provided a new idea for the further development and utilization of soybean protein.					
32161592	6	0	theme	dendritic	957:965	arg1	cells					967:971	human monocyte-derived dendritic cells	934:971	human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands	934:1020	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	1	1	theme	glycan	263:268	arg1	structures					270:279	specific tumor-associated glycan structures	237:279	specific tumor-associated glycan structures	237:279	Many tumors display alterations in the biosynthetic pathways of glycosylation, resulting in increased expression of specific tumor-associated glycan structures.					
32161592	6	2	theme	human	934:938	arg1	cells					967:971	human monocyte-derived dendritic cells	934:971	human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands	934:1020	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	8	3	theme	flux	1216:1219	arg1	analysis					1221:1228	extracellular flux analysis	1202:1228	extracellular flux analysis	1202:1228	In concordance with this, extracellular flux analysis confirmed the decrease in glycolytic activity upon MGL triggering in human dendritic cells.					
32161592	1	4	theme	biosynthetic	160:171	arg1	pathways					173:180	the biosynthetic pathways	156:180	the biosynthetic pathways of glycosylation	156:197	Many tumors display alterations in the biosynthetic pathways of glycosylation, resulting in increased expression of specific tumor-associated glycan structures.					
32161592	8	5	theme	human	1299:1303	arg1	cells					1315:1319	human dendritic cells	1299:1319	human dendritic cells	1299:1319	In concordance with this, extracellular flux analysis confirmed the decrease in glycolytic activity upon MGL triggering in human dendritic cells.					
32161592	5	6	theme	immune	752:757	arg1	responses					759:767	immune responses	752:767	immune responses	752:767	Although these findings indicate that MGL engagement by glycan ligands can modulate immune responses, the impact of MGL ligation on dendritic cells is still not completely understood.					
32161592	2	7	theme	glycan	310:315	arg1	structures					317:326	these altered glycan structures	296:326	these altered glycan structures	296:326	Expression of these altered glycan structures is associated with metastasis and poor prognosis.					
32161592	8	8	theme	glycolytic	1256:1265	arg1	activity					1267:1274	glycolytic activity	1256:1274	glycolytic activity	1256:1274	In concordance with this, extracellular flux analysis confirmed the decrease in glycolytic activity upon MGL triggering in human dendritic cells.					
32161592	6	9	theme	MGL	1003:1005	arg1	ligands					1014:1020	two different MGL glycan ligands	989:1020	two different MGL glycan ligands	989:1020	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	10	10	from	impact	1493:1498	arg1	metabolism					1558:1567	metabolism	1558:1567	metabolism	1558:1567	Overall, our findings highlight the impact of tumor-associated glycans on dendritic cell biology and metabolism and will increase our understanding on how glycans can shape immunity.					
32161592	10	10	from	impact	1493:1498	arg1	biology					1546:1552	dendritic cell biology	1531:1552	dendritic cell biology	1531:1552	Overall, our findings highlight the impact of tumor-associated glycans on dendritic cell biology and metabolism and will increase our understanding on how glycans can shape immunity.					
32161592	5	11	theme	glycan	724:729	arg1	ligands					731:737	glycan ligands	724:737	glycan ligands	724:737	Although these findings indicate that MGL engagement by glycan ligands can modulate immune responses, the impact of MGL ligation on dendritic cells is still not completely understood.					
32161592	7	12	theme	reduced	1047:1053	arg1	expression					1055:1064	a reduced expression	1045:1064	a reduced expression of genes coding for key enzymes involved in the glycolysis pathway, TCA cycle, and oxidative phosphorylation	1045:1173	Our analyses revealed a reduced expression of genes coding for key enzymes involved in the glycolysis pathway, TCA cycle, and oxidative phosphorylation.					
32161592	5	13	theme	MGL	706:708	arg1	engagement					710:719	MGL engagement	706:719	MGL engagement by glycan ligands	706:737	Although these findings indicate that MGL engagement by glycan ligands can modulate immune responses, the impact of MGL ligation on dendritic cells is still not completely understood.					
32161592	1	14	theme	Many	121:124	arg1	tumors					126:131	Many tumors	121:131	Many tumors	121:131	Many tumors display alterations in the biosynthetic pathways of glycosylation, resulting in increased expression of specific tumor-associated glycan structures.					
32161592	0	15	theme	GalNAc	48:53	arg1	Ligands					55:61	Terminal GalNAc Ligands	39:61	Terminal GalNAc Ligands	39:61	Activation of the C-Type Lectin MGL by Terminal GalNAc Ligands Reduces the Glycolytic Activity of Human Dendritic Cells.					
32161592	4	16	theme	antigen-mediated	532:547	arg1	activation					549:558	Tn antigen-mediated activation	529:558	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells	529:602	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	1	17	from	alterations	141:151	arg1	pathways					173:180	the biosynthetic pathways	156:180	the biosynthetic pathways of glycosylation	156:197	Many tumors display alterations in the biosynthetic pathways of glycosylation, resulting in increased expression of specific tumor-associated glycan structures.					
32161592	3	18	theme	Antigen	378:384	arg1	cells					397:401	Antigen presenting cells	378:401	Antigen presenting cells	378:401	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	0	19	theme	Glycolytic	75:84	arg1	Activity					86:93	the Glycolytic Activity	71:93	the Glycolytic Activity of Human Dendritic Cells	71:118	Activation of the C-Type Lectin MGL by Terminal GalNAc Ligands Reduces the Glycolytic Activity of Human Dendritic Cells.					
32161592	10	20	theme	glycans	1520:1526	arg1	impact					1493:1498	the impact	1489:1498	the impact of tumor-associated glycans on dendritic cell biology and metabolism	1489:1567	Overall, our findings highlight the impact of tumor-associated glycans on dendritic cell biology and metabolism and will increase our understanding on how glycans can shape immunity.					
32161592	3	21	theme	O-glycan	477:484	arg1	antigen					489:495	the truncated O-glycan Tn antigen	463:495	the truncated O-glycan Tn antigen	463:495	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	5	22	theme	MGL	784:786	arg1	ligation					788:795	MGL ligation	784:795	MGL ligation	784:795	Although these findings indicate that MGL engagement by glycan ligands can modulate immune responses, the impact of MGL ligation on dendritic cells is still not completely understood.					
32161592	4	23	theme	dendritic	588:596	arg1	cells					598:602	dendritic cells	588:602	dendritic cells	588:602	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	9	24	theme	diminished	1369:1378	arg1	activity					1391:1398	a diminished glycolytic activity	1367:1398	a diminished glycolytic activity of human dendritic cells upon C-type lectin stimulation	1367:1454	To our knowledge, we are the first to report a diminished glycolytic activity of human dendritic cells upon C-type lectin stimulation.					
32161592	10	25	theme	dendritic	1531:1539	arg1	biology					1546:1552	dendritic cell biology	1531:1552	dendritic cell biology	1531:1552	Overall, our findings highlight the impact of tumor-associated glycans on dendritic cell biology and metabolism and will increase our understanding on how glycans can shape immunity.					
32161592	0	26	theme	Lectin	25:30	arg1	MGL					32:34	the C-Type Lectin MGL	14:34	the C-Type Lectin MGL	14:34	Activation of the C-Type Lectin MGL by Terminal GalNAc Ligands Reduces the Glycolytic Activity of Human Dendritic Cells.					
32161592	3	27	theme	glycan	434:439	arg1	structures					441:450	tumor-associated glycan structures	417:450	tumor-associated glycan structures	417:450	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	3	27	theme	glycan	434:439	arg1	antigen					489:495	the truncated O-glycan Tn antigen	463:495	the truncated O-glycan Tn antigen	463:495	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	4	28	theme	C-type	567:572	arg1	MGL					581:583	the C-type lectin MGL	563:583	the C-type lectin MGL	563:583	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	2	29	theme	poor	362:365	arg1	prognosis					367:375	poor prognosis	362:375	poor prognosis	362:375	Expression of these altered glycan structures is associated with metastasis and poor prognosis.					
32161592	9	30	theme	human	1403:1407	arg1	cells					1419:1423	human dendritic cells	1403:1423	human dendritic cells	1403:1423	To our knowledge, we are the first to report a diminished glycolytic activity of human dendritic cells upon C-type lectin stimulation.					
32161592	4	31	theme	MGL	581:583	arg1	activation					549:558	Tn antigen-mediated activation	529:558	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells	529:602	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	3	32	theme	glycan	511:516	arg1	receptors					518:526	specific glycan receptors	502:526	specific glycan receptors	502:526	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	1	33	theme	increased	213:221	arg1	expression					223:232	increased expression	213:232	increased expression of specific tumor-associated glycan structures	213:279	Many tumors display alterations in the biosynthetic pathways of glycosylation, resulting in increased expression of specific tumor-associated glycan structures.					
32161592	4	34	theme	enhanced	639:646	arg1	secretion					648:656	the enhanced secretion	635:656	the enhanced secretion of IL-10	635:665	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	9	35	theme	C-type	1430:1435	arg1	stimulation					1444:1454	C-type lectin stimulation	1430:1454	C-type lectin stimulation	1430:1454	To our knowledge, we are the first to report a diminished glycolytic activity of human dendritic cells upon C-type lectin stimulation.					
32161592	8	36	from	decrease	1244:1251	arg1	cells					1315:1319	human dendritic cells	1299:1319	human dendritic cells	1299:1319	In concordance with this, extracellular flux analysis confirmed the decrease in glycolytic activity upon MGL triggering in human dendritic cells.					
32161592	8	36	from	decrease	1244:1251	arg1	activity					1267:1274	glycolytic activity	1256:1274	glycolytic activity	1256:1274	In concordance with this, extracellular flux analysis confirmed the decrease in glycolytic activity upon MGL triggering in human dendritic cells.					
32161592	7	37	theme	oxidative	1149:1157	arg1	phosphorylation					1159:1173	oxidative phosphorylation	1149:1173	oxidative phosphorylation	1149:1173	Our analyses revealed a reduced expression of genes coding for key enzymes involved in the glycolysis pathway, TCA cycle, and oxidative phosphorylation.					
32161592	1	38	theme	tumor-associated	246:261	arg1	structures					270:279	specific tumor-associated glycan structures	237:279	specific tumor-associated glycan structures	237:279	Many tumors display alterations in the biosynthetic pathways of glycosylation, resulting in increased expression of specific tumor-associated glycan structures.					
32161592	6	39	theme	monocyte-derived	940:955	arg1	cells					967:971	human monocyte-derived dendritic cells	934:971	human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands	934:1020	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	4	40	theme	T	623:623	arg1	cells					625:629	regulatory T cells	612:629	regulatory T cells	612:629	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	4	41	from	activation	549:558	arg1	cells					598:602	dendritic cells	588:602	dendritic cells	588:602	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	2	42	theme	altered	302:308	arg1	structures					317:326	these altered glycan structures	296:326	these altered glycan structures	296:326	Expression of these altered glycan structures is associated with metastasis and poor prognosis.					
32161592	1	43	theme	structures	270:279	arg1	expression					223:232	increased expression	213:232	increased expression of specific tumor-associated glycan structures	213:279	Many tumors display alterations in the biosynthetic pathways of glycosylation, resulting in increased expression of specific tumor-associated glycan structures.					
32161592	7	44	theme	TCA	1134:1136	arg1	cycle					1138:1142	TCA cycle	1134:1142	TCA cycle	1134:1142	Our analyses revealed a reduced expression of genes coding for key enzymes involved in the glycolysis pathway, TCA cycle, and oxidative phosphorylation.					
32161592	8	45	theme	extracellular	1202:1214	arg1	analysis					1221:1228	extracellular flux analysis	1202:1228	extracellular flux analysis	1202:1228	In concordance with this, extracellular flux analysis confirmed the decrease in glycolytic activity upon MGL triggering in human dendritic cells.					
32161592	6	46	theme	pathway	914:920	arg1	analysis					922:929	pathway analysis	914:929	pathway analysis	914:929	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	8	47	theme	MGL	1281:1283	arg1	triggering					1285:1294	MGL triggering	1281:1294	MGL triggering	1281:1294	In concordance with this, extracellular flux analysis confirmed the decrease in glycolytic activity upon MGL triggering in human dendritic cells.					
32161592	0	48	theme	Cells	114:118	arg1	Activity					86:93	the Glycolytic Activity	71:93	the Glycolytic Activity of Human Dendritic Cells	71:118	Activation of the C-Type Lectin MGL by Terminal GalNAc Ligands Reduces the Glycolytic Activity of Human Dendritic Cells.					
32161592	2	49	theme	structures	317:326	arg1	Expression					282:291	Expression	282:291	Expression of these altered glycan structures	282:326	Expression of these altered glycan structures is associated with metastasis and poor prognosis.					
32161592	1	50	theme	glycosylation	185:197	arg1	pathways					173:180	the biosynthetic pathways	156:180	the biosynthetic pathways of glycosylation	156:197	Many tumors display alterations in the biosynthetic pathways of glycosylation, resulting in increased expression of specific tumor-associated glycan structures.					
32161592	6	51	theme	glycan	1007:1012	arg1	ligands					1014:1020	two different MGL glycan ligands	989:1020	two different MGL glycan ligands	989:1020	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	8	52	theme	dendritic	1305:1313	arg1	cells					1315:1319	human dendritic cells	1299:1319	human dendritic cells	1299:1319	In concordance with this, extracellular flux analysis confirmed the decrease in glycolytic activity upon MGL triggering in human dendritic cells.					
32161592	6	53	theme	different	993:1001	arg1	ligands					1014:1020	two different MGL glycan ligands	989:1020	two different MGL glycan ligands	989:1020	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	7	54	theme	key	1086:1088	arg1	enzymes					1090:1096	key enzymes	1086:1096	key enzymes involved in the glycolysis pathway, TCA cycle, and oxidative phosphorylation	1086:1173	Our analyses revealed a reduced expression of genes coding for key enzymes involved in the glycolysis pathway, TCA cycle, and oxidative phosphorylation.					
32161592	5	55	from	impact	774:779	arg1	cells					810:814	dendritic cells	800:814	dendritic cells	800:814	Although these findings indicate that MGL engagement by glycan ligands can modulate immune responses, the impact of MGL ligation on dendritic cells is still not completely understood.					
32161592	7	56	theme	genes	1069:1073	arg1	expression					1055:1064	a reduced expression	1045:1064	a reduced expression of genes coding for key enzymes involved in the glycolysis pathway, TCA cycle, and oxidative phosphorylation	1045:1173	Our analyses revealed a reduced expression of genes coding for key enzymes involved in the glycolysis pathway, TCA cycle, and oxidative phosphorylation.					
32161592	5	57	theme	ligation	788:795	arg1	impact					774:779	the impact	770:779	the impact of MGL ligation on dendritic cells	770:814	Although these findings indicate that MGL engagement by glycan ligands can modulate immune responses, the impact of MGL ligation on dendritic cells is still not completely understood.					
32161592	9	58	theme	cells	1419:1423	arg1	activity					1391:1398	a diminished glycolytic activity	1367:1398	a diminished glycolytic activity of human dendritic cells upon C-type lectin stimulation	1367:1454	To our knowledge, we are the first to report a diminished glycolytic activity of human dendritic cells upon C-type lectin stimulation.					
32161592	5	59	theme	dendritic	800:808	arg1	cells					810:814	dendritic cells	800:814	dendritic cells	800:814	Although these findings indicate that MGL engagement by glycan ligands can modulate immune responses, the impact of MGL ligation on dendritic cells is still not completely understood.					
32161592	7	60	theme	glycolysis	1114:1123	arg1	pathway					1125:1131	the glycolysis pathway	1110:1131	the glycolysis pathway	1110:1131	Our analyses revealed a reduced expression of genes coding for key enzymes involved in the glycolysis pathway, TCA cycle, and oxidative phosphorylation.					
32161592	3	61	theme	presenting	386:395	arg1	cells					397:401	Antigen presenting cells	378:401	Antigen presenting cells	378:401	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	8	62	dep	this	1196:1199	arg1	concordance					1179:1189	concordance	1179:1189	concordance	1179:1189	In concordance with this, extracellular flux analysis confirmed the decrease in glycolytic activity upon MGL triggering in human dendritic cells.					
32161592	0	63	theme	Human	98:102	arg1	Cells					114:118	Human Dendritic Cells	98:118	Human Dendritic Cells	98:118	Activation of the C-Type Lectin MGL by Terminal GalNAc Ligands Reduces the Glycolytic Activity of Human Dendritic Cells.					
32161592	4	64	theme	Tn	529:530	arg1	activation					549:558	Tn antigen-mediated activation	529:558	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells	529:602	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	10	65	theme	tumor-associated	1503:1518	arg1	glycans					1520:1526	tumor-associated glycans	1503:1526	tumor-associated glycans	1503:1526	Overall, our findings highlight the impact of tumor-associated glycans on dendritic cell biology and metabolism and will increase our understanding on how glycans can shape immunity.					
32161592	3	66	theme	Tn	486:487	arg1	antigen					489:495	the truncated O-glycan Tn antigen	463:495	the truncated O-glycan Tn antigen	463:495	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	3	67	theme	tumor-associated	417:432	arg1	structures					441:450	tumor-associated glycan structures	417:450	tumor-associated glycan structures	417:450	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	3	67	theme	tumor-associated	417:432	arg1	antigen					489:495	the truncated O-glycan Tn antigen	463:495	the truncated O-glycan Tn antigen	463:495	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	6	68	dep	employed	866:873	arg1	GO					891:892	GO	891:892	GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands	891:1020	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	3	69	theme	truncated	467:475	arg1	antigen					489:495	the truncated O-glycan Tn antigen	463:495	the truncated O-glycan Tn antigen	463:495	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	0	70	theme	C-Type	18:23	arg1	MGL					32:34	the C-Type Lectin MGL	14:34	the C-Type Lectin MGL	14:34	Activation of the C-Type Lectin MGL by Terminal GalNAc Ligands Reduces the Glycolytic Activity of Human Dendritic Cells.					
32161592	4	71	theme	regulatory	612:621	arg1	cells					625:629	regulatory T cells	612:629	regulatory T cells	612:629	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	10	72	theme	cell	1541:1544	arg1	biology					1546:1552	dendritic cell biology	1531:1552	dendritic cell biology	1531:1552	Overall, our findings highlight the impact of tumor-associated glycans on dendritic cell biology and metabolism and will increase our understanding on how glycans can shape immunity.					
32161592	3	73	theme	specific	502:509	arg1	receptors					518:526	specific glycan receptors	502:526	specific glycan receptors	502:526	Antigen presenting cells can recognize tumor-associated glycan structures, including the truncated O-glycan Tn antigen, via specific glycan receptors.					
32161592	0	74	theme	MGL	32:34	arg1	Activation					0:9	Activation	0:9	Activation of the C-Type Lectin MGL by Terminal GalNAc Ligands	0:61	Activation of the C-Type Lectin MGL by Terminal GalNAc Ligands Reduces the Glycolytic Activity of Human Dendritic Cells.					
32161592	9	75	theme	glycolytic	1380:1389	arg1	activity					1391:1398	a diminished glycolytic activity	1367:1398	a diminished glycolytic activity of human dendritic cells upon C-type lectin stimulation	1367:1454	To our knowledge, we are the first to report a diminished glycolytic activity of human dendritic cells upon C-type lectin stimulation.					
32161592	6	76	theme	term	894:897	arg1	enrichment					899:908	term enrichment	894:908	term enrichment	894:908	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	6	77	link	monocyte-derived	940:955	arg1	cells					967:971	human monocyte-derived dendritic cells	934:971	human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands	934:1020	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	4	78	theme	lectin	574:579	arg1	MGL					581:583	the C-type lectin MGL	563:583	the C-type lectin MGL	563:583	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	0	79	theme	Terminal	39:46	arg1	Ligands					55:61	Terminal GalNAc Ligands	39:61	Terminal GalNAc Ligands	39:61	Activation of the C-Type Lectin MGL by Terminal GalNAc Ligands Reduces the Glycolytic Activity of Human Dendritic Cells.					
32161592	9	80	theme	dendritic	1409:1417	arg1	cells					1419:1423	human dendritic cells	1403:1423	human dendritic cells	1403:1423	To our knowledge, we are the first to report a diminished glycolytic activity of human dendritic cells upon C-type lectin stimulation.					
32161592	6	81	theme	RNA	875:877	arg1	sequencing					879:888	RNA sequencing	875:888	RNA sequencing	875:888	Therefore, we employed RNA sequencing, GO term enrichment and pathway analysis on human monocyte-derived dendritic cells stimulated with two different MGL glycan ligands.					
32161592	0	82	theme	Dendritic	104:112	arg1	Cells					114:118	Human Dendritic Cells	98:118	Human Dendritic Cells	98:118	Activation of the C-Type Lectin MGL by Terminal GalNAc Ligands Reduces the Glycolytic Activity of Human Dendritic Cells.					
32161592	4	83	theme	IL-10	661:665	arg1	secretion					648:656	the enhanced secretion	635:656	the enhanced secretion of IL-10	635:665	Tn antigen-mediated activation of the C-type lectin MGL on dendritic cells induces regulatory T cells via the enhanced secretion of IL-10.					
32161592	1	84	theme	specific	237:244	arg1	structures					270:279	specific tumor-associated glycan structures	237:279	specific tumor-associated glycan structures	237:279	Many tumors display alterations in the biosynthetic pathways of glycosylation, resulting in increased expression of specific tumor-associated glycan structures.					
32161592	9	85	theme	lectin	1437:1442	arg1	stimulation					1444:1454	C-type lectin stimulation	1430:1454	C-type lectin stimulation	1430:1454	To our knowledge, we are the first to report a diminished glycolytic activity of human dendritic cells upon C-type lectin stimulation.					
34859056	9	0	theme	basic	1660:1664	arg1	pathway					1679:1685	the basic biosynthetic pathway	1656:1685	the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures	1656:1754	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	9	0	theme	basic	1660:1664	arg1	well-conserved					1759:1772	well-conserved	1759:1772	well-conserved	1759:1772	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	9	1	theme	monosaccharide	1928:1941	arg1	composition					1943:1953	their monosaccharide composition	1922:1953	their monosaccharide composition	1922:1953	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	1	2	theme	structures	248:257	arg1	diversity					228:236	diversity	228:236	diversity	228:236	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	1	2	theme	structures	248:257	arg1	function					215:222	function	215:222	function	215:222	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	4	3	contain	have	712:715	arg1	glycoproteomics					676:690	glycoproteomics	676:690	glycoproteomics	676:690	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	4	3	contain	have	712:715	arg2	efforts					740:746	advanced efforts	731:746	advanced efforts	731:746	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	4	3	contain	have	712:715	arg1	glycomics					697:705	glycomics	697:705	glycomics	697:705	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	4	3	contain	have	712:715	arg1	proteomics					664:673	proteomics	664:673	proteomics	664:673	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	7	4	theme	released	1462:1469	arg1	N-glycans					1471:1479	released N-glycans	1462:1479	released N-glycans	1462:1479	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	10	5	theme	species-normative	2160:2176	arg1	baselines					2178:2186	species-normative baselines	2160:2186	species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses	2160:2313	Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses.					
34859056	9	6	theme	pattern	1968:1974	arg1	terms					1913:1917	terms	1913:1917	terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation	1913:2017	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	3	7	theme	logistic	511:518	arg1	complications					520:532	the logistic complications	507:532	the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens	507:607	To date, the glycomes of only a handful of non-human, non-domesticated vertebrates have been analyzed in depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens.					
34859056	4	8	from	advances	652:659	arg1	glycoproteomics					676:690	glycoproteomics	676:690	glycoproteomics	676:690	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	4	8	from	advances	652:659	arg1	glycomics					697:705	glycomics	697:705	glycomics	697:705	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	4	8	from	advances	652:659	arg1	proteomics					664:673	proteomics	664:673	proteomics	664:673	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	2	9	theme	health	367:372	arg1	research					379:386	non-human health care research	357:386	non-human health care research	357:386	These strategies can be equally well applied to advance non-human health care research.					
34859056	9	10	theme	fucosylation	1977:1988	arg1	terms					1913:1917	terms	1913:1917	terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation	1913:2017	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	1	11	theme	rapid	182:186	arg1	progress					188:195	rapid progress	182:195	rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease	182:298	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	9	12	theme	glycoprotein	1720:1731	arg1	structures					1745:1754	glycoprotein glycan core structures	1720:1754	glycoprotein glycan core structures	1720:1754	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	7	13	theme	resulting	1368:1376	arg1	preparations					1391:1402	the resulting protein-rich preparations	1364:1402	the resulting protein-rich preparations	1364:1402	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	10	14	theme	pathogen	2243:2250	arg1	invasion					2252:2259	pathogen invasion	2243:2259	pathogen invasion	2243:2259	Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses.					
34859056	4	15	theme	last	627:630	arg1	2 decades					632:640	the last 2 decades	623:640	the last 2 decades	623:640	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	9	16	gly	sialylation	1956:1966	arg1	O-acetylation					2005:2017	O-acetylation	2005:2017	O-acetylation	2005:2017	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	5	17	theme	N-glycan	843:850	arg1	diversity					863:871	N-glycan structural diversity	843:871	N-glycan structural diversity	843:871	In this study, we investigated N-glycan structural diversity in serum harvested from five cultured fish species.					
34859056	9	18	theme	core	1740:1743	arg1	structures					1745:1754	glycoprotein glycan core structures	1720:1754	glycoprotein glycan core structures	1720:1754	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	10	19	theme	new	2052:2054	arg1	contributions					2056:2068	new contributions	2052:2068	new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses	2052:2313	Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses.					
34859056	6	20	theme	starting	951:958	arg1	point					960:964	a useful starting point	942:964	a useful starting point for glycomic analysis	942:986	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	6	20	theme	starting	951:958	arg1	biofluid					930:937	This biofluid	925:937	This biofluid	925:937	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	7	21	theme	organic	1341:1347	arg1	extraction					1349:1358	organic extraction	1341:1358	organic extraction	1341:1358	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	10	22	theme	growing	2075:2081	arg1	library					2083:2089	a growing library	2073:2089	a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses	2073:2313	Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses.					
34859056	1	23	theme	mass	128:131	arg1	techniques					147:156	mass spectrometric techniques	128:156	mass spectrometric techniques	128:156	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	9	24	theme	teleost	1785:1791	arg1	species					1798:1804	the teleost fish species	1781:1804	the teleost fish species examined in this study	1781:1827	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	7	25	theme	Arctic	1287:1292	arg1	char					1294:1297	Arctic char	1287:1297	Arctic char	1287:1297	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	7	25	theme	Arctic	1287:1292	arg1	species					1257:1263	three teleost fish species	1238:1263	three teleost fish species	1238:1263	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	5	26	theme	cultured	902:909	arg1	species					916:922	five cultured fish species	897:922	five cultured fish species	897:922	In this study, we investigated N-glycan structural diversity in serum harvested from five cultured fish species.					
34859056	0	27	from	Diversity	16:24	arg1	Fishes					55:60	Teleost and Chondrostrean Fishes	29:60	Fishes	55:60	Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.					
34859056	7	28	theme	shortnose	1214:1222	arg1	sturgeon					1224:1231	the Atlantic sturgeon and shortnose sturgeon	1188:1231	sturgeon	1224:1231	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	6	29	dep	rich	1002:1005	arg1	acquired					1032:1039	acquired	1032:1039	can be acquired in a sustainable fashion	1025:1064	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	4	30	theme	serum/plasma	766:777	arg1	biomarkers					779:788	human serum/plasma biomarkers	760:788	human serum/plasma biomarkers for various diseases	760:809	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	1	31	theme	Recent	63:68	arg1	advances					70:77	Recent advances	63:77	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques	63:156	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	9	32	theme	species-specific	1830:1845	arg1	differences					1858:1868	species-specific structural differences	1830:1868	species-specific structural differences	1830:1868	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	0	33	theme	Serum	0:4	arg1	Diversity					16:24	Serum N-Glycome Diversity	0:24	Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.	0:61	Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.					
34859056	1	34	theme	human	275:279	arg1	health					281:286	human health	275:286	human health	275:286	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	7	35	theme	chondrostrean	1160:1172	arg1	species					1179:1185	two chondrostrean fish species	1156:1185	two chondrostrean fish species	1156:1185	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	1	36	theme	chemical	106:113	arg1	biology					115:121	chemical biology	106:121	chemical biology	106:121	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	10	37	theme	N-glycosylation	2202:2216	arg1	dynamics					2218:2225	N-glycosylation dynamics	2202:2225	N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses	2202:2313	Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses.					
34859056	0	38	theme	Teleost	29:35	arg1	Fishes					55:60	Teleost and Chondrostrean Fishes	29:60	Fishes	55:60	Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.					
34859056	8	39	theme	nanospray	1581:1589	arg1	spectrometry					1607:1618	nanospray ionization mass spectrometry	1581:1618	nanospray ionization mass spectrometry in negative or positive mode	1581:1647	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	6	40	from	changes	1112:1118	arg1	organism					1127:1134	the organism	1123:1134	the organism	1123:1134	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	0	41	theme	Chondrostrean	41:53	arg1	Fishes					55:60	Teleost and Chondrostrean Fishes	29:60	Fishes	55:60	Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.					
34859056	7	42	theme	Atlantic	1270:1277	arg1	salmon					1279:1284	the Atlantic salmon	1266:1284	the Atlantic salmon	1266:1284	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	7	42	theme	Atlantic	1270:1277	arg1	species					1257:1263	three teleost fish species	1238:1263	three teleost fish species	1238:1263	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	8	43	theme	mass	1602:1605	arg1	spectrometry					1607:1618	nanospray ionization mass spectrometry	1581:1618	nanospray ionization mass spectrometry in negative or positive mode	1581:1647	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	3	44	theme	vertebrates	460:470	arg1	handful					421:427	only a handful	414:427	only a handful of non-human, non-domesticated vertebrates	414:470	To date, the glycomes of only a handful of non-human, non-domesticated vertebrates have been analyzed in depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens.					
34859056	3	45	dep	non-human	432:440	arg1	non-domesticated					443:458	non-domesticated	443:458	non-domesticated	443:458	To date, the glycomes of only a handful of non-human, non-domesticated vertebrates have been analyzed in depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens.					
34859056	3	46	theme	due	500:502	arg1	depth					494:498	depth	494:498	depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens	494:607	To date, the glycomes of only a handful of non-human, non-domesticated vertebrates have been analyzed in depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens.					
34859056	7	47	theme	structural	1485:1494	arg1	analysis					1496:1503	structural analysis	1485:1503	structural analysis	1485:1503	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	6	48	theme	dynamic	1092:1098	arg1	changes					1112:1118	dynamic physiologic changes	1092:1118	dynamic physiologic changes in the organism	1092:1134	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	1	49	theme	glycan	241:246	arg1	structures					248:257	glycan structures	241:257	glycan structures associated with human health and disease	241:298	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	9	50	theme	biosynthetic	1666:1677	arg1	pathway					1679:1685	the basic biosynthetic pathway	1656:1685	the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures	1656:1754	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	9	50	theme	biosynthetic	1666:1677	arg1	well-conserved					1759:1772	well-conserved	1759:1772	well-conserved	1759:1772	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	9	51	theme	composition	1943:1953	arg1	terms					1913:1917	terms	1913:1917	terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation	1913:2017	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	9	52	theme	sialylation	1956:1966	arg1	pattern					1968:1974	sialylation pattern	1956:1974	sialylation pattern	1956:1974	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	8	53	from	spectrometry	1607:1618	arg1	mode					1644:1647	negative or positive mode	1623:1647	negative or positive mode	1623:1647	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	9	54	gly	glycoprotein	1720:1731	arg1	glycoprotein					1720:1731	glycoprotein glycan core structures	1720:1754	glycoprotein glycan core structures	1720:1754	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	6	55	theme	glycomic	970:977	arg1	analysis					979:986	glycomic analysis	970:986	glycomic analysis	970:986	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	9	56	theme	glycan	1733:1738	arg1	structures					1745:1754	glycoprotein glycan core structures	1720:1754	glycoprotein glycan core structures	1720:1754	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	7	57	theme	protein-rich	1378:1389	arg1	preparations					1391:1402	the resulting protein-rich preparations	1364:1402	the resulting protein-rich preparations	1364:1402	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	9	58	theme	degree	1995:2000	arg1	terms					1913:1917	terms	1913:1917	terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation	1913:2017	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	9	59	gly	fucosylation	1977:1988	arg1	O-acetylation					2005:2017	O-acetylation	2005:2017	O-acetylation	2005:2017	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	3	60	theme	farm-raised	587:597	arg1	specimens					599:607	or handling wild-caught or farm-raised specimens	560:607	or handling wild-caught or farm-raised specimens	560:607	To date, the glycomes of only a handful of non-human, non-domesticated vertebrates have been analyzed in depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens.					
34859056	5	61	theme	structural	852:861	arg1	diversity					863:871	N-glycan structural diversity	843:871	N-glycan structural diversity	843:871	In this study, we investigated N-glycan structural diversity in serum harvested from five cultured fish species.					
34859056	9	62	theme	structures	1745:1754	arg1	production					1706:1715	the production	1702:1715	the production of glycoprotein glycan core structures	1702:1754	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	1	63	dep	function	215:222	arg1	the					211:213	the	211:213	the	211:213	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	9	64	theme	O-acetylation	2005:2017	arg1	degree					1995:2000	degree	1995:2000	degree	1995:2000	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	9	64	theme	O-acetylation	2005:2017	arg1	fucosylation					1977:1988	fucosylation	1977:1988	fucosylation	1977:1988	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	9	64	theme	O-acetylation	2005:2017	arg1	composition					1943:1953	their monosaccharide composition	1922:1953	their monosaccharide composition	1922:1953	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	9	64	theme	O-acetylation	2005:2017	arg1	pattern					1968:1974	sialylation pattern	1956:1974	sialylation pattern	1956:1974	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	3	65	theme	wild-caught	572:582	arg1	specimens					599:607	or handling wild-caught or farm-raised specimens	560:607	or handling wild-caught or farm-raised specimens	560:607	To date, the glycomes of only a handful of non-human, non-domesticated vertebrates have been analyzed in depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens.					
34859056	6	66	theme	useful	944:949	arg1	point					960:964	a useful starting point	942:964	a useful starting point for glycomic analysis	942:986	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	6	66	theme	useful	944:949	arg1	biofluid					930:937	This biofluid	925:937	This biofluid	925:937	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	10	67	theme	environmental	2262:2274	arg1	stress					2276:2281	environmental stress	2262:2281	environmental stress	2262:2281	Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses.					
34859056	9	68	from	organisms	1900:1908	arg1	terms					1913:1917	terms	1913:1917	terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation	1913:2017	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	1	69	theme	spectrometric	133:145	arg1	techniques					147:156	mass spectrometric techniques	128:156	mass spectrometric techniques	128:156	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	9	70	theme	fish	1793:1796	arg1	species					1798:1804	the teleost fish species	1781:1804	the teleost fish species examined in this study	1781:1827	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	8	71	theme	Released	1506:1513	arg1	N-glycans					1515:1523	Released N-glycans	1506:1523	Released N-glycans	1506:1523	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	8	71	theme	Released	1506:1513	arg1	forms					1572:1576	their native or permethylated forms	1542:1576	their native or permethylated forms	1542:1576	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	10	72	theme	fish	2288:2291	arg1	responses					2305:2313	fish immunologic responses	2288:2313	fish immunologic responses	2288:2313	Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses.					
34859056	5	73	theme	fish	911:914	arg1	species					916:922	five cultured fish species	897:922	five cultured fish species	897:922	In this study, we investigated N-glycan structural diversity in serum harvested from five cultured fish species.					
34859056	6	74	from	glycoproteins	1010:1022	arg1	rich					1002:1005	rich	1002:1005	rich	1002:1005	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	10	75	theme	datasets	2094:2101	arg1	library					2083:2089	a growing library	2073:2089	a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses	2073:2313	Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses.					
34859056	7	76	theme	Atlantic	1192:1199	arg1	sturgeon					1201:1208	the Atlantic sturgeon and shortnose sturgeon	1188:1231	sturgeon	1201:1208	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	4	77	theme	human	760:764	arg1	biomarkers					779:788	human serum/plasma biomarkers	760:788	human serum/plasma biomarkers for various diseases	760:809	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	10	78	theme	immunologic	2293:2303	arg1	responses					2305:2313	fish immunologic responses	2288:2313	fish immunologic responses	2288:2313	Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses.					
34859056	7	79	theme	channel	1304:1310	arg1	species					1257:1263	three teleost fish species	1238:1263	three teleost fish species	1238:1263	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	7	79	theme	channel	1304:1310	arg1	catfish					1312:1318	channel catfish	1304:1318	channel catfish	1304:1318	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	8	80	theme	native	1548:1553	arg1	forms					1572:1576	their native or permethylated forms	1542:1576	their native or permethylated forms	1542:1576	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	8	80	theme	native	1548:1553	arg1	N-glycans					1515:1523	Released N-glycans	1506:1523	Released N-glycans	1506:1523	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	3	81	theme	handful	421:427	arg1	glycomes					402:409	the glycomes	398:409	the glycomes of only a handful of non-human, non-domesticated vertebrates	398:470	To date, the glycomes of only a handful of non-human, non-domesticated vertebrates have been analyzed in depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens.					
34859056	0	82	theme	N-Glycome	6:14	arg1	Diversity					16:24	Serum N-Glycome Diversity	0:24	Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.	0:61	Serum N-Glycome Diversity in Teleost and Chondrostrean Fishes.					
34859056	1	83	theme	carbohydrate	82:93	arg1	chemistry					95:103	carbohydrate chemistry	82:103	carbohydrate chemistry	82:103	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	9	84	theme	structural	1847:1856	arg1	differences					1858:1868	species-specific structural differences	1830:1868	species-specific structural differences	1830:1868	While the basic biosynthetic pathway that initiates the production of glycoprotein glycan core structures is well-conserved across the teleost fish species examined in this study, species-specific structural differences were detected across the five organisms in terms of their monosaccharide composition, sialylation pattern, fucosylation, and degree of O-acetylation.					
34859056	8	85	theme	permethylated	1558:1570	arg1	forms					1572:1576	their native or permethylated forms	1542:1576	their native or permethylated forms	1542:1576	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	8	85	theme	permethylated	1558:1570	arg1	N-glycans					1515:1523	Released N-glycans	1506:1523	Released N-glycans	1506:1523	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	6	86	from	rich	1002:1005	arg1	glycoproteins					1010:1022	glycoproteins	1010:1022	glycoproteins	1010:1022	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	4	87	theme	advanced	731:738	arg1	efforts					740:746	advanced efforts	731:746	advanced efforts	731:746	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	2	88	theme	care	374:377	arg1	research					379:386	non-human health care research	357:386	non-human health care research	357:386	These strategies can be equally well applied to advance non-human health care research.					
34859056	6	89	theme	sustainable	1046:1056	arg1	fashion					1058:1064	a sustainable fashion	1044:1064	a sustainable fashion	1044:1064	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	6	90	theme	physiologic	1100:1110	arg1	changes					1112:1118	dynamic physiologic changes	1092:1118	dynamic physiologic changes in the organism	1092:1134	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	8	91	theme	ionization	1591:1600	arg1	spectrometry					1607:1618	nanospray ionization mass spectrometry	1581:1618	nanospray ionization mass spectrometry in negative or positive mode	1581:1647	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	3	92	theme	non-human	432:440	arg1	vertebrates					460:470	non-human, non-domesticated vertebrates	432:470	non-human, non-domesticated vertebrates	432:470	To date, the glycomes of only a handful of non-human, non-domesticated vertebrates have been analyzed in depth due to the logistic complications associated with obtaining or handling wild-caught or farm-raised specimens.					
34859056	2	93	theme	non-human	357:365	arg1	research					379:386	non-human health care research	357:386	non-human health care research	357:386	These strategies can be equally well applied to advance non-human health care research.					
34859056	8	94	theme	negative	1623:1630	arg1	mode					1644:1647	negative or positive mode	1623:1647	negative or positive mode	1623:1647	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	4	95	theme	various	794:800	arg1	diseases					802:809	various diseases	794:809	various diseases	794:809	In contrast, the last 2 decades have seen advances in proteomics, glycoproteomics, and glycomics that have significantly advanced efforts to identify human serum/plasma biomarkers for various diseases.					
34859056	7	96	theme	fish	1252:1255	arg1	salmon					1279:1284	the Atlantic salmon	1266:1284	the Atlantic salmon	1266:1284	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	7	96	theme	fish	1252:1255	arg1	char					1294:1297	Arctic char	1287:1297	Arctic char	1287:1297	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	7	96	theme	fish	1252:1255	arg1	species					1257:1263	three teleost fish species	1238:1263	three teleost fish species	1238:1263	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	7	96	theme	fish	1252:1255	arg1	catfish					1312:1318	channel catfish	1304:1318	channel catfish	1304:1318	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	7	97	theme	teleost	1244:1250	arg1	salmon					1279:1284	the Atlantic salmon	1266:1284	the Atlantic salmon	1266:1284	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	7	97	theme	teleost	1244:1250	arg1	char					1294:1297	Arctic char	1287:1297	Arctic char	1287:1297	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	7	97	theme	teleost	1244:1250	arg1	species					1257:1263	three teleost fish species	1238:1263	three teleost fish species	1238:1263	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	7	97	theme	teleost	1244:1250	arg1	catfish					1312:1318	channel catfish	1304:1318	channel catfish	1304:1318	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	10	98	theme	fish	2114:2117	arg1	N-glycomes					2119:2128	fish N-glycomes	2114:2128	fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses	2114:2313	Our methods and results provide new contributions to a growing library of datasets describing fish N-glycomes that can eventually establish species-normative baselines for assessing N-glycosylation dynamics associated with pathogen invasion, environmental stress, and fish immunologic responses.					
34859056	8	99	theme	positive	1635:1642	arg1	mode					1644:1647	negative or positive mode	1623:1647	negative or positive mode	1623:1647	Released N-glycans were analyzed as their native or permethylated forms by nanospray ionization mass spectrometry in negative or positive mode.					
34859056	6	100	gly	glycoproteins	1010:1022	arg1	glycoproteins					1010:1022	glycoproteins	1010:1022	glycoproteins	1010:1022	This biofluid is a useful starting point for glycomic analysis because it is rich in glycoproteins, can be acquired in a sustainable fashion, and its contents reflect dynamic physiologic changes in the organism.					
34859056	7	101	theme	fish	1174:1177	arg1	species					1179:1185	two chondrostrean fish species	1156:1185	two chondrostrean fish species	1156:1185	Sera acquired from two chondrostrean fish species, the Atlantic sturgeon and shortnose sturgeon, and three teleost fish species, the Atlantic salmon, Arctic char, and channel catfish, were delipidated by organic extraction and the resulting protein-rich preparations sequentially treated with trypsin and PNGaseF to generate released N-glycans for structural analysis.					
34859056	1	102	from	advances	70:77	arg1	chemistry					95:103	carbohydrate chemistry	82:103	carbohydrate chemistry	82:103	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	1	102	from	advances	70:77	arg1	biology					115:121	chemical biology	106:121	chemical biology	106:121	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
34859056	1	102	from	advances	70:77	arg1	techniques					147:156	mass spectrometric techniques	128:156	mass spectrometric techniques	128:156	Recent advances in carbohydrate chemistry, chemical biology, and mass spectrometric techniques have opened the door to rapid progress in uncovering the function and diversity of glycan structures associated with human health and disease.					
32213588	6	0	theme	extended	1050:1057	arg1	glycosylation					1072:1084	extended high-mannose glycosylation	1050:1084	extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1	1050:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	1	theme	protein	1132:1138	arg1	protein					1132:1138	transferrin receptor protein 1	1111:1140	transferrin receptor protein 1	1111:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	1	theme	protein	1132:1138	arg1	domain					1101:1106	the helical domain	1089:1106	the helical domain of transferrin receptor protein 1	1089:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	7	2	from	present	1358:1364	arg1	proteins					1390:1397	cancer cell membrane proteins	1369:1397	cancer cell membrane proteins	1369:1397	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	4	3	theme	gene	613:616	arg1	MAN1A1					618:623	the Golgi α-mannosidase I coding gene MAN1A1	580:623	the Golgi α-mannosidase I coding gene MAN1A1	580:623	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	7	4	theme	therapeutic	1412:1422	arg1	targets					1424:1430	therapeutic targets	1412:1430	therapeutic targets for preventing metastasis	1412:1456	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	7	4	theme	therapeutic	1412:1422	arg1	present					1358:1364	α-1,2-mannosylated N-glycans present	1329:1364	α-1,2-mannosylated N-glycans present on cancer cell membrane proteins	1329:1397	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	6	5	from	enhancement	1232:1242	arg1	cholangiocarcinoma					1276:1293	metastatic cholangiocarcinoma	1265:1293	metastatic cholangiocarcinoma	1265:1293	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	6	theme	migration	1252:1260	arg1	enhancement					1232:1242	enhancement	1232:1242	enhancement of cell migration in metastatic cholangiocarcinoma	1232:1293	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	4	7	theme	coding	606:611	arg1	MAN1A1					618:623	the Golgi α-mannosidase I coding gene MAN1A1	580:623	the Golgi α-mannosidase I coding gene MAN1A1	580:623	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	2	8	theme	cell	315:318	arg1	type					320:323	cell type	315:323	cell type	315:323	These structures can undergo dynamic changes in composition and expression based on cell type, external stimuli, and genetic factors.					
32213588	4	9	theme	α-1,2-mannose	697:709	arg1	residues					711:718	terminating α-1,2-mannose residues	685:718	terminating α-1,2-mannose residues	685:718	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	6	10	theme	modeling	1026:1033	arg1	elucidation					949:959	Exclusive elucidation	939:959	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling	939:1033	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	11	gly	glycosylation	1072:1084	arg2	protein					1132:1138	transferrin receptor protein 1	1111:1140	transferrin receptor protein 1	1111:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	11	gly	glycosylation	1072:1084	arg1	protein					1132:1138	transferrin receptor protein 1	1111:1140	transferrin receptor protein 1	1111:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	11	gly	glycosylation	1072:1084	arg2	domain					1101:1106	the helical domain	1089:1106	the helical domain of transferrin receptor protein 1	1089:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	11	gly	glycosylation	1072:1084	arg1	domain					1101:1106	the helical domain	1089:1106	the helical domain of transferrin receptor protein 1	1089:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	4	12	theme	MAN1A1	618:623	arg1	down-regulation					561:575	down-regulation	561:575	down-regulation of the Golgi α-mannosidase I coding gene MAN1A1	561:623	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	7	13	theme	membrane	1381:1388	arg1	proteins					1390:1397	cancer cell membrane proteins	1369:1397	cancer cell membrane proteins	1369:1397	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	6	14	theme	molecular	1016:1024	arg1	modeling					1026:1033	molecular modeling	1016:1033	molecular modeling	1016:1033	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	15	theme	cell	1247:1250	arg1	migration					1252:1260	cell migration	1247:1260	cell migration	1247:1260	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	1	16	theme	Membrane-bound	79:92	arg1	oligosaccharides					94:109	Membrane-bound oligosaccharides	79:109	Membrane-bound oligosaccharides	79:109	Membrane-bound oligosaccharides form the interfacial boundary between the cell and its environment, mediating processes such as adhesion and signaling.					
32213588	3	17	theme	incurable	456:464	arg1	forms					466:470	presently incurable forms	446:470	presently incurable forms of advanced cancer	446:489	Glycosylation, therefore, is a promising target of therapeutic interventions for presently incurable forms of advanced cancer.					
32213588	5	18	theme	cell	870:873	arg1	mannosylation					883:895	masking cell surface mannosylation	862:895	masking cell surface mannosylation	862:895	Subsequent reshaping of the glycome by inhibiting α-mannosidase I resulted in significantly higher migratory and invasive capabilities while masking cell surface mannosylation suppressed metastasis-related phenotypes.					
32213588	6	19	theme	conformational	1151:1164	arg1	changes					1166:1172	conformational changes	1151:1172	conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma	1151:1293	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	7	20	theme	cell	1376:1379	arg1	proteins					1390:1397	cancer cell membrane proteins	1369:1397	cancer cell membrane proteins	1369:1397	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	4	21	theme	terminating	685:695	arg1	residues					711:718	terminating α-1,2-mannose residues	685:718	terminating α-1,2-mannose residues	685:718	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	5	22	theme	surface	875:881	arg1	mannosylation					883:895	masking cell surface mannosylation	862:895	masking cell surface mannosylation	862:895	Subsequent reshaping of the glycome by inhibiting α-mannosidase I resulted in significantly higher migratory and invasive capabilities while masking cell surface mannosylation suppressed metastasis-related phenotypes.					
32213588	6	23	theme	glycoproteins	998:1010	arg1	elucidation					949:959	Exclusive elucidation	939:959	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling	939:1033	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	24	theme	receptor	1123:1130	arg1	protein					1132:1138	transferrin receptor protein 1	1111:1140	transferrin receptor protein 1	1111:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	7	25	theme	cancer	1369:1374	arg1	proteins					1390:1397	cancer cell membrane proteins	1369:1397	cancer cell membrane proteins	1369:1397	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	3	26	theme	promising	396:404	arg1	Glycosylation					365:377	Glycosylation	365:377	Glycosylation	365:377	Glycosylation, therefore, is a promising target of therapeutic interventions for presently incurable forms of advanced cancer.					
32213588	3	26	theme	promising	396:404	arg1	target					406:411	a promising target	394:411	a promising target of therapeutic interventions for presently incurable forms of advanced cancer	394:489	Glycosylation, therefore, is a promising target of therapeutic interventions for presently incurable forms of advanced cancer.					
32213588	4	27	theme	Golgi	584:588	arg1	MAN1A1					618:623	the Golgi α-mannosidase I coding gene MAN1A1	580:623	the Golgi α-mannosidase I coding gene MAN1A1	580:623	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	7	28	theme	α-1,2-mannosylated	1329:1346	arg1	targets					1424:1430	therapeutic targets	1412:1430	therapeutic targets for preventing metastasis	1412:1456	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	7	28	theme	α-1,2-mannosylated	1329:1346	arg1	present					1358:1364	α-1,2-mannosylated N-glycans present	1329:1364	α-1,2-mannosylated N-glycans present on cancer cell membrane proteins	1329:1397	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	2	29	theme	external	326:333	arg1	stimuli					335:341	external stimuli	326:341	external stimuli	326:341	These structures can undergo dynamic changes in composition and expression based on cell type, external stimuli, and genetic factors.					
32213588	6	30	theme	transferrin	1111:1121	arg1	protein					1132:1138	transferrin receptor protein 1	1111:1140	transferrin receptor protein 1	1111:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	1	31	theme	interfacial	120:130	arg1	boundary					132:139	the interfacial boundary	116:139	the interfacial boundary between the cell and its environment	116:176	Membrane-bound oligosaccharides form the interfacial boundary between the cell and its environment, mediating processes such as adhesion and signaling.					
32213588	2	32	from	changes	268:274	arg1	expression					295:304	expression	295:304	expression	295:304	These structures can undergo dynamic changes in composition and expression based on cell type, external stimuli, and genetic factors.					
32213588	2	32	from	changes	268:274	arg1	composition					279:289	composition	279:289	composition	279:289	These structures can undergo dynamic changes in composition and expression based on cell type, external stimuli, and genetic factors.					
32213588	3	33	theme	cancer	484:489	arg1	forms					466:470	presently incurable forms	446:470	presently incurable forms of advanced cancer	446:489	Glycosylation, therefore, is a promising target of therapeutic interventions for presently incurable forms of advanced cancer.					
32213588	0	34	theme	cholangiocarcinoma	14:31	arg1	Metastasis					0:9	Metastasis	0:9	Metastasis of cholangiocarcinoma	0:31	Metastasis of cholangiocarcinoma is promoted by extended high-mannose glycans.					
32213588	4	35	theme	I	604:604	arg1	MAN1A1					618:623	the Golgi α-mannosidase I coding gene MAN1A1	580:623	the Golgi α-mannosidase I coding gene MAN1A1	580:623	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	6	36	from	domain	1101:1106	arg1	glycosylation					1072:1084	extended high-mannose glycosylation	1050:1084	extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1	1050:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	37	theme	membrane	989:996	arg1	glycoproteins					998:1010	differentially expressed membrane glycoproteins	964:1010	differentially expressed membrane glycoproteins	964:1010	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	7	38	theme	N-glycans	1348:1356	arg1	targets					1424:1430	therapeutic targets	1412:1430	therapeutic targets for preventing metastasis	1412:1456	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	7	38	theme	N-glycans	1348:1356	arg1	present					1358:1364	α-1,2-mannosylated N-glycans present	1329:1364	α-1,2-mannosylated N-glycans present on cancer cell membrane proteins	1329:1397	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	4	39	theme	extended	650:657	arg1	glycans					672:678	extended high-mannose glycans	650:678	extended high-mannose glycans with terminating α-1,2-mannose residues	650:718	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	3	40	theme	therapeutic	416:426	arg1	interventions					428:440	therapeutic interventions	416:440	therapeutic interventions	416:440	Glycosylation, therefore, is a promising target of therapeutic interventions for presently incurable forms of advanced cancer.					
32213588	4	41	theme	α-mannosidase	590:602	arg1	MAN1A1					618:623	the Golgi α-mannosidase I coding gene MAN1A1	580:623	the Golgi α-mannosidase I coding gene MAN1A1	580:623	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	5	42	dep	higher	813:818	arg1	migratory					820:828	migratory	820:828	migratory	820:828	Subsequent reshaping of the glycome by inhibiting α-mannosidase I resulted in significantly higher migratory and invasive capabilities while masking cell surface mannosylation suppressed metastasis-related phenotypes.					
32213588	5	42	dep	higher	813:818	arg1	invasive					834:841	invasive	834:841	invasive	834:841	Subsequent reshaping of the glycome by inhibiting α-mannosidase I resulted in significantly higher migratory and invasive capabilities while masking cell surface mannosylation suppressed metastasis-related phenotypes.					
32213588	6	43	theme	expressed	979:987	arg1	glycoproteins					998:1010	differentially expressed membrane glycoproteins	964:1010	differentially expressed membrane glycoproteins	964:1010	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	2	44	theme	dynamic	260:266	arg1	changes					268:274	dynamic changes	260:274	dynamic changes in composition and expression based on cell type, external stimuli, and genetic factors	260:362	These structures can undergo dynamic changes in composition and expression based on cell type, external stimuli, and genetic factors.					
32213588	3	45	theme	interventions	428:440	arg1	Glycosylation					365:377	Glycosylation	365:377	Glycosylation	365:377	Glycosylation, therefore, is a promising target of therapeutic interventions for presently incurable forms of advanced cancer.					
32213588	3	45	theme	interventions	428:440	arg1	target					406:411	a promising target	394:411	a promising target of therapeutic interventions for presently incurable forms of advanced cancer	394:489	Glycosylation, therefore, is a promising target of therapeutic interventions for presently incurable forms of advanced cancer.					
32213588	6	46	theme	helical	1093:1099	arg1	protein					1132:1138	transferrin receptor protein 1	1111:1140	transferrin receptor protein 1	1111:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	46	theme	helical	1093:1099	arg1	domain					1101:1106	the helical domain	1089:1106	the helical domain of transferrin receptor protein 1	1089:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	5	47	theme	α-mannosidase	771:783	arg1	I					785:785	α-mannosidase I	771:785	α-mannosidase I	771:785	Subsequent reshaping of the glycome by inhibiting α-mannosidase I resulted in significantly higher migratory and invasive capabilities while masking cell surface mannosylation suppressed metastasis-related phenotypes.					
32213588	7	48	gly	α-1,2-mannosylated	1329:1346	arg1	targets					1424:1430	therapeutic targets	1412:1430	therapeutic targets for preventing metastasis	1412:1456	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	7	48	gly	α-1,2-mannosylated	1329:1346	arg1	present					1358:1364	α-1,2-mannosylated N-glycans present	1329:1364	α-1,2-mannosylated N-glycans present on cancer cell membrane proteins	1329:1397	The results provide support that α-1,2-mannosylated N-glycans present on cancer cell membrane proteins may serve as therapeutic targets for preventing metastasis.					
32213588	3	49	theme	advanced	475:482	arg1	cancer					484:489	advanced cancer	475:489	advanced cancer	475:489	Glycosylation, therefore, is a promising target of therapeutic interventions for presently incurable forms of advanced cancer.					
32213588	0	50	theme	high-mannose	57:68	arg1	glycans					70:76	extended high-mannose glycans	48:76	extended high-mannose glycans	48:76	Metastasis of cholangiocarcinoma is promoted by extended high-mannose glycans.					
32213588	6	51	theme	interaction	1199:1209	arg1	energies					1211:1218	noncovalent interaction energies	1187:1218	noncovalent interaction energies	1187:1218	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	0	52	theme	extended	48:55	arg1	glycans					70:76	extended high-mannose glycans	48:76	extended high-mannose glycans	48:76	Metastasis of cholangiocarcinoma is promoted by extended high-mannose glycans.					
32213588	6	53	theme	high-mannose	1059:1070	arg1	glycosylation					1072:1084	extended high-mannose glycosylation	1050:1084	extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1	1050:1140	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	54	theme	Exclusive	939:947	arg1	elucidation					949:959	Exclusive elucidation	939:959	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling	939:1033	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	2	55	theme	genetic	348:354	arg1	factors					356:362	genetic factors	348:362	genetic factors	348:362	These structures can undergo dynamic changes in composition and expression based on cell type, external stimuli, and genetic factors.					
32213588	4	56	with	glycans	672:678	arg1	residues					711:718	terminating α-1,2-mannose residues	685:718	terminating α-1,2-mannose residues	685:718	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	1	57	theme	cell	153:156	arg1	environment					166:176	the cell and its environment	149:176	environment	166:176	Membrane-bound oligosaccharides form the interfacial boundary between the cell and its environment, mediating processes such as adhesion and signaling.					
32213588	6	58	theme	noncovalent	1187:1197	arg1	energies					1211:1218	noncovalent interaction energies	1187:1218	noncovalent interaction energies	1187:1218	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	6	59	gly	glycoproteins	998:1010	arg1	glycoproteins					998:1010	differentially expressed membrane glycoproteins	964:1010	differentially expressed membrane glycoproteins	964:1010	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32213588	5	60	theme	higher	813:818	arg1	capabilities					843:854	significantly higher migratory and invasive capabilities	799:854	significantly higher migratory and invasive capabilities	799:854	Subsequent reshaping of the glycome by inhibiting α-mannosidase I resulted in significantly higher migratory and invasive capabilities while masking cell surface mannosylation suppressed metastasis-related phenotypes.					
32213588	4	61	theme	glycans	672:678	arg1	elevation					637:645	elevation	637:645	elevation of extended high-mannose glycans with terminating α-1,2-mannose residues	637:718	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	4	62	theme	high-mannose	659:670	arg1	glycans					672:678	extended high-mannose glycans	650:678	extended high-mannose glycans with terminating α-1,2-mannose residues	650:718	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	1	63	theme	its	162:164	arg1	environment					166:176	the cell and its environment	149:176	environment	166:176	Membrane-bound oligosaccharides form the interfacial boundary between the cell and its environment, mediating processes such as adhesion and signaling.					
32213588	5	64	theme	masking	862:868	arg1	mannosylation					883:895	masking cell surface mannosylation	862:895	masking cell surface mannosylation	862:895	Subsequent reshaping of the glycome by inhibiting α-mannosidase I resulted in significantly higher migratory and invasive capabilities while masking cell surface mannosylation suppressed metastasis-related phenotypes.					
32213588	4	65	theme	cholangiocarcinoma	511:528	arg1	metastasis					530:539	cholangiocarcinoma metastasis	511:539	cholangiocarcinoma metastasis	511:539	Here, we show that cholangiocarcinoma metastasis is characterized by down-regulation of the Golgi α-mannosidase I coding gene MAN1A1, leading to elevation of extended high-mannose glycans with terminating α-1,2-mannose residues.					
32213588	5	66	theme	metastasis-related	908:925	arg1	phenotypes					927:936	metastasis-related phenotypes	908:936	metastasis-related phenotypes	908:936	Subsequent reshaping of the glycome by inhibiting α-mannosidase I resulted in significantly higher migratory and invasive capabilities while masking cell surface mannosylation suppressed metastasis-related phenotypes.					
32213588	6	67	theme	metastatic	1265:1274	arg1	cholangiocarcinoma					1276:1293	metastatic cholangiocarcinoma	1265:1293	metastatic cholangiocarcinoma	1265:1293	Exclusive elucidation of differentially expressed membrane glycoproteins and molecular modeling suggested that extended high-mannose glycosylation at the helical domain of transferrin receptor protein 1 promotes conformational changes that improve noncovalent interaction energies and lead to enhancement of cell migration in metastatic cholangiocarcinoma.					
32753748	0	0	theme	plasma	73:78	arg1	glycosylation					80:92	plasma glycosylation	73:92	plasma glycosylation	73:92	The schizophrenia risk locus in SLC39A8 alters brain metal transport and plasma glycosylation.					
32753748	5	1	theme	N-glycome	766:774	arg1	profiling					776:784	plasma protein N-glycome profiling	751:784	plasma protein N-glycome profiling	751:784	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	3	2	theme	SLC39A8	512:518	arg1	allele					529:534	the common SLC39A8 missense allele	501:534	the common SLC39A8 missense allele	501:534	Here, we identified several Mn-related changes in human carriers of the common SLC39A8 missense allele.					
32753748	7	3	theme	schizophrenia	1175:1187	arg1	pathogenesis					1189:1200	schizophrenia pathogenesis	1175:1200	schizophrenia pathogenesis	1175:1200	Characterizing the functional impact of this variant will enhance our understanding of schizophrenia pathogenesis and identify novel therapeutic targets and biomarkers.					
32753748	2	4	theme	due	355:357	arg1	Mn					302:303	Mn	302:303	Mn	302:303	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	2	4	theme	due	355:357	arg1	manganese					291:299	undetectable serum manganese	272:299	undetectable serum manganese (Mn)	272:304	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	4	5	theme	dose-dependent	585:598	arg1	change					600:605	a dose-dependent change	583:605	a dose-dependent change in the ratio of T2w to T1w signal in several regions	583:658	Analysis of structural brain MRI scans showed a dose-dependent change in the ratio of T2w to T1w signal in several regions.					
32753748	5	6	theme	serum	737:741	arg1	Mn					743:744	only serum Mn	732:744	only serum Mn	732:744	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	5	7	theme	Comprehensive	661:673	arg1	analysis					689:696	Comprehensive trace element analysis	661:696	Comprehensive trace element analysis	661:696	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	6	8	theme	N-glycome	829:837	arg1	profiling					839:847	N-glycome profiling	829:847	N-glycome profiling from two individuals with SLC39A8-CDG	829:885	N-glycome profiling from two individuals with SLC39A8-CDG showed similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility.					
32753748	5	9	theme	Mn	743:744	arg1	reduction					719:727	a specific reduction	708:727	a specific reduction of only serum Mn	708:744	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	4	10	theme	T1w	630:632	arg1	signal					634:639	T1w signal	630:639	T1w signal in several regions	630:658	Analysis of structural brain MRI scans showed a dose-dependent change in the ratio of T2w to T1w signal in several regions.					
32753748	5	11	theme	trace	675:679	arg1	analysis					689:696	Comprehensive trace element analysis	661:696	Comprehensive trace element analysis	661:696	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	6	12	theme	similar	894:900	arg1	alterations					918:928	similar but more severe alterations	894:928	similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility	894:1085	N-glycome profiling from two individuals with SLC39A8-CDG showed similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility.					
32753748	4	13	from	change	600:605	arg1	ratio					614:618	the ratio	610:618	the ratio of T2w to T1w signal in several regions	610:658	Analysis of structural brain MRI scans showed a dose-dependent change in the ratio of T2w to T1w signal in several regions.					
32753748	3	14	theme	human	483:487	arg1	carriers					489:496	human carriers	483:496	human carriers of the common SLC39A8 missense allele	483:534	Here, we identified several Mn-related changes in human carriers of the common SLC39A8 missense allele.					
32753748	2	15	from	mutations	241:249	arg1	SLC39A8					254:260	SLC39A8	254:260	SLC39A8	254:260	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	6	16	with	spectrum	1029:1036	arg1	potential					1059:1067	potential	1059:1067	potential for reversibility	1059:1085	N-glycome profiling from two individuals with SLC39A8-CDG showed similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility.					
32753748	6	17	from	alterations	918:928	arg1	branching					933:941	branching	933:941	branching	933:941	N-glycome profiling from two individuals with SLC39A8-CDG showed similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility.					
32753748	6	18	theme	severe	911:916	arg1	alterations					918:928	similar but more severe alterations	894:928	similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility	894:1085	N-glycome profiling from two individuals with SLC39A8-CDG showed similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility.					
32753748	6	19	theme	common	1002:1007	arg1	variant					1009:1015	the common variant	998:1015	the common variant	998:1015	N-glycome profiling from two individuals with SLC39A8-CDG showed similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility.					
32753748	7	20	theme	pathogenesis	1189:1200	arg1	understanding					1158:1170	our understanding	1154:1170	our understanding of schizophrenia pathogenesis	1154:1200	Characterizing the functional impact of this variant will enhance our understanding of schizophrenia pathogenesis and identify novel therapeutic targets and biomarkers.					
32753748	2	21	theme	glycosyltransferases	391:410	arg1	sensitivity					376:386	the exquisite sensitivity	362:386	the exquisite sensitivity of glycosyltransferases to Mn concentration	362:430	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	0	22	theme	schizophrenia	4:16	arg1	locus					23:27	The schizophrenia risk locus	0:27	The schizophrenia risk locus in SLC39A8	0:38	The schizophrenia risk locus in SLC39A8 alters brain metal transport and plasma glycosylation.					
32753748	1	23	theme	several	182:188	arg1	phenotypes					201:210	several additional phenotypes	182:210	several additional phenotypes	182:210	A common missense variant in SLC39A8 is convincingly associated with schizophrenia and several additional phenotypes.					
32753748	7	24	theme	novel	1215:1219	arg1	targets					1233:1239	novel therapeutic targets	1215:1239	novel therapeutic targets	1215:1239	Characterizing the functional impact of this variant will enhance our understanding of schizophrenia pathogenesis and identify novel therapeutic targets and biomarkers.					
32753748	4	25	theme	scans	570:574	arg1	Analysis					537:544	Analysis	537:544	Analysis of structural brain MRI scans	537:574	Analysis of structural brain MRI scans showed a dose-dependent change in the ratio of T2w to T1w signal in several regions.					
32753748	6	26	theme	Mn	962:963	arg1	supplementation					965:979	Mn supplementation	962:979	Mn supplementation	962:979	N-glycome profiling from two individuals with SLC39A8-CDG showed similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility.					
32753748	7	27	theme	functional	1107:1116	arg1	impact					1118:1123	the functional impact	1103:1123	the functional impact of this variant	1103:1139	Characterizing the functional impact of this variant will enhance our understanding of schizophrenia pathogenesis and identify novel therapeutic targets and biomarkers.					
32753748	1	28	theme	additional	190:199	arg1	phenotypes					201:210	several additional phenotypes	182:210	several additional phenotypes	182:210	A common missense variant in SLC39A8 is convincingly associated with schizophrenia and several additional phenotypes.					
32753748	7	29	theme	therapeutic	1221:1231	arg1	targets					1233:1239	novel therapeutic targets	1215:1239	novel therapeutic targets	1215:1239	Characterizing the functional impact of this variant will enhance our understanding of schizophrenia pathogenesis and identify novel therapeutic targets and biomarkers.					
32753748	4	30	theme	MRI	566:568	arg1	scans					570:574	structural brain MRI scans	549:574	structural brain MRI scans	549:574	Analysis of structural brain MRI scans showed a dose-dependent change in the ratio of T2w to T1w signal in several regions.					
32753748	5	31	theme	specific	710:717	arg1	reduction					719:727	a specific reduction	708:727	a specific reduction of only serum Mn	708:744	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	1	32	from	variant	113:119	arg1	SLC39A8					124:130	SLC39A8	124:130	SLC39A8	124:130	A common missense variant in SLC39A8 is convincingly associated with schizophrenia and several additional phenotypes.					
32753748	2	33	theme	Congenital	312:321	arg1	Disorder					323:330	a Congenital Disorder	310:330	a Congenital Disorder of Glycosylation (CDG)	310:353	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	0	34	theme	risk	18:21	arg1	locus					23:27	The schizophrenia risk locus	0:27	The schizophrenia risk locus in SLC39A8	0:38	The schizophrenia risk locus in SLC39A8 alters brain metal transport and plasma glycosylation.					
32753748	1	35	theme	common	97:102	arg1	variant					113:119	A common missense variant	95:119	A common missense variant in SLC39A8	95:130	A common missense variant in SLC39A8 is convincingly associated with schizophrenia and several additional phenotypes.					
32753748	4	36	from	signal	634:639	arg1	regions					652:658	several regions	644:658	several regions	644:658	Analysis of structural brain MRI scans showed a dose-dependent change in the ratio of T2w to T1w signal in several regions.					
32753748	3	37	theme	allele	529:534	arg1	carriers					489:496	human carriers	483:496	human carriers of the common SLC39A8 missense allele	483:534	Here, we identified several Mn-related changes in human carriers of the common SLC39A8 missense allele.					
32753748	4	38	theme	several	644:650	arg1	regions					652:658	several regions	644:658	several regions	644:658	Analysis of structural brain MRI scans showed a dose-dependent change in the ratio of T2w to T1w signal in several regions.					
32753748	3	39	theme	Mn-related	461:470	arg1	changes					472:478	several Mn-related changes	453:478	several Mn-related changes in human carriers of the common SLC39A8 missense allele	453:534	Here, we identified several Mn-related changes in human carriers of the common SLC39A8 missense allele.					
32753748	5	40	theme	reduced	795:801	arg1	complexity					803:812	complexity	803:812	complexity	803:812	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	5	41	theme	only	732:735	arg1	Mn					743:744	only serum Mn	732:744	only serum Mn	732:744	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	2	42	theme	Mn	415:416	arg1	concentration					418:430	Mn concentration	415:430	Mn concentration	415:430	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	0	43	theme	brain	47:51	arg1	transport					59:67	brain metal transport	47:67	brain metal transport	47:67	The schizophrenia risk locus in SLC39A8 alters brain metal transport and plasma glycosylation.					
32753748	5	44	theme	element	681:687	arg1	analysis					689:696	Comprehensive trace element analysis	661:696	Comprehensive trace element analysis	661:696	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	5	45	theme	protein	758:764	arg1	profiling					776:784	plasma protein N-glycome profiling	751:784	plasma protein N-glycome profiling	751:784	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	3	46	from	changes	472:478	arg1	carriers					489:496	human carriers	483:496	human carriers of the common SLC39A8 missense allele	483:534	Here, we identified several Mn-related changes in human carriers of the common SLC39A8 missense allele.					
32753748	1	47	theme	missense	104:111	arg1	variant					113:119	A common missense variant	95:119	A common missense variant in SLC39A8	95:130	A common missense variant in SLC39A8 is convincingly associated with schizophrenia and several additional phenotypes.					
32753748	6	48	from	individuals	858:868	arg1	profiling					839:847	N-glycome profiling	829:847	N-glycome profiling from two individuals with SLC39A8-CDG	829:885	N-glycome profiling from two individuals with SLC39A8-CDG showed similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility.					
32753748	4	49	theme	brain	560:564	arg1	scans					570:574	structural brain MRI scans	549:574	structural brain MRI scans	549:574	Analysis of structural brain MRI scans showed a dose-dependent change in the ratio of T2w to T1w signal in several regions.					
32753748	2	50	theme	loss-of-function	224:239	arg1	mutations					241:249	Homozygous loss-of-function mutations	213:249	Homozygous loss-of-function mutations in SLC39A8	213:260	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	3	51	theme	missense	520:527	arg1	allele					529:534	the common SLC39A8 missense allele	501:534	the common SLC39A8 missense allele	501:534	Here, we identified several Mn-related changes in human carriers of the common SLC39A8 missense allele.					
32753748	3	52	theme	common	505:510	arg1	allele					529:534	the common SLC39A8 missense allele	501:534	the common SLC39A8 missense allele	501:534	Here, we identified several Mn-related changes in human carriers of the common SLC39A8 missense allele.					
32753748	4	53	theme	structural	549:558	arg1	scans					570:574	structural brain MRI scans	549:574	structural brain MRI scans	549:574	Analysis of structural brain MRI scans showed a dose-dependent change in the ratio of T2w to T1w signal in several regions.					
32753748	2	54	theme	Homozygous	213:222	arg1	mutations					241:249	Homozygous loss-of-function mutations	213:249	Homozygous loss-of-function mutations in SLC39A8	213:260	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	2	55	theme	serum	285:289	arg1	Mn					302:303	Mn	302:303	Mn	302:303	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	2	55	theme	serum	285:289	arg1	manganese					291:299	undetectable serum manganese	272:299	undetectable serum manganese (Mn)	272:304	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	2	56	theme	Glycosylation	335:347	arg1	Disorder					323:330	a Congenital Disorder	310:330	a Congenital Disorder of Glycosylation (CDG)	310:353	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	2	56	theme	Glycosylation	335:347	arg1	Mn					302:303	Mn	302:303	Mn	302:303	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	2	56	theme	Glycosylation	335:347	arg1	manganese					291:299	undetectable serum manganese	272:299	undetectable serum manganese (Mn)	272:304	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	7	57	theme	variant	1133:1139	arg1	impact					1118:1123	the functional impact	1103:1123	the functional impact of this variant	1103:1139	Characterizing the functional impact of this variant will enhance our understanding of schizophrenia pathogenesis and identify novel therapeutic targets and biomarkers.					
32753748	5	58	theme	plasma	751:756	arg1	profiling					776:784	plasma protein N-glycome profiling	751:784	plasma protein N-glycome profiling	751:784	Comprehensive trace element analysis confirmed a specific reduction of only serum Mn, and plasma protein N-glycome profiling revealed reduced complexity and branching.					
32753748	6	59	with	individuals	858:868	arg1	SLC39A8-CDG					875:885	SLC39A8-CDG	875:885	SLC39A8-CDG	875:885	N-glycome profiling from two individuals with SLC39A8-CDG showed similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility.					
32753748	2	60	theme	undetectable	272:283	arg1	Mn					302:303	Mn	302:303	Mn	302:303	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	2	60	theme	undetectable	272:283	arg1	manganese					291:299	undetectable serum manganese	272:299	undetectable serum manganese (Mn)	272:304	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	2	61	theme	exquisite	366:374	arg1	sensitivity					376:386	the exquisite sensitivity	362:386	the exquisite sensitivity of glycosyltransferases to Mn concentration	362:430	Homozygous loss-of-function mutations in SLC39A8 result in undetectable serum manganese (Mn) and a Congenital Disorder of Glycosylation (CDG) due to the exquisite sensitivity of glycosyltransferases to Mn concentration.					
32753748	3	62	theme	several	453:459	arg1	changes					472:478	several Mn-related changes	453:478	several Mn-related changes in human carriers of the common SLC39A8 missense allele	453:534	Here, we identified several Mn-related changes in human carriers of the common SLC39A8 missense allele.					
32753748	6	63	theme	hypofunction	1041:1052	arg1	spectrum					1029:1036	a spectrum	1027:1036	a spectrum of hypofunction with potential for reversibility	1027:1085	N-glycome profiling from two individuals with SLC39A8-CDG showed similar but more severe alterations in branching that improved with Mn supplementation, suggesting that the common variant exists on a spectrum of hypofunction with potential for reversibility.					
32753748	0	64	theme	metal	53:57	arg1	transport					59:67	brain metal transport	47:67	brain metal transport	47:67	The schizophrenia risk locus in SLC39A8 alters brain metal transport and plasma glycosylation.					
32753748	0	65	from	locus	23:27	arg1	SLC39A8					32:38	SLC39A8	32:38	SLC39A8	32:38	The schizophrenia risk locus in SLC39A8 alters brain metal transport and plasma glycosylation.					
32753748	4	66	theme	T2w	623:625	arg1	ratio					614:618	the ratio	610:618	the ratio of T2w to T1w signal in several regions	610:658	Analysis of structural brain MRI scans showed a dose-dependent change in the ratio of T2w to T1w signal in several regions.					
31964828	0	0	theme	complex	72:78	arg1	N-glycan					80:87	a specific complex N-glycan	61:87	a specific complex N-glycan	61:87	Receptor recognition by meningococcal type IV pili relies on a specific complex N-glycan.					
31964828	4	1	theme	type	547:550	arg1	factor					582:587	a key adhesive factor	567:587	a key adhesive factor present in numerous bacterial pathogens	567:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	1	theme	type	547:550	arg1	Tfp					561:563	Tfp	561:563	Tfp	561:563	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	1	theme	type	547:550	arg1	pili					555:558	type IV pili	547:558	type IV pili (Tfp)	547:564	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	7	2	theme	antibacterial	1239:1251	arg1	target					1253:1258	a potential antibacterial target	1227:1258	a potential antibacterial target	1227:1258	These findings reveal the molecular basis of the selective receptor recognition by meningococcal Tfp and thereby, identify a potential antibacterial target.					
31964828	7	3	theme	selective	1153:1161	arg1	recognition					1172:1182	the selective receptor recognition	1149:1182	the selective receptor recognition by meningococcal Tfp	1149:1203	These findings reveal the molecular basis of the selective receptor recognition by meningococcal Tfp and thereby, identify a potential antibacterial target.					
31964828	4	4	theme	numerous	600:607	arg1	pathogens					619:627	numerous bacterial pathogens	600:627	numerous bacterial pathogens	600:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	0	5	theme	specific	63:70	arg1	N-glycan					80:87	a specific complex N-glycan	61:87	a specific complex N-glycan	61:87	Receptor recognition by meningococcal type IV pili relies on a specific complex N-glycan.					
31964828	1	6	theme	lectin-like	150:160	arg1	adhesins					162:169	lectin-like adhesins	150:169	lectin-like adhesins	150:169	Bacterial infections are frequently based on the binding of lectin-like adhesins to specific glycan determinants exposed on host cell receptors.					
31964828	4	7	from	pathogens	619:627	arg1	present					589:595	present	589:595	present	589:595	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	8	theme	pili	555:558	arg1	recognition					532:542	the receptor recognition	519:542	the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens	519:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	2	9	theme	particular	306:315	arg1	tissues					322:328	particular host tissues	306:328	particular host tissues	306:328	These interactions confer species-specific recognition and tropism for particular host tissues and represent attractive antibacterial targets.					
31964828	3	10	theme	wide	391:394	arg1	diversity					407:415	the wide structural diversity	387:415	the wide structural diversity of carbohydrates	387:432	However, the wide structural diversity of carbohydrates hampers the characterization of specific glycan determinants.					
31964828	4	11	theme	receptor	523:530	arg1	recognition					532:542	the receptor recognition	519:542	the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens	519:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	7	12	theme	potential	1229:1237	arg1	target					1253:1258	a potential antibacterial target	1227:1258	a potential antibacterial target	1227:1258	These findings reveal the molecular basis of the selective receptor recognition by meningococcal Tfp and thereby, identify a potential antibacterial target.					
31964828	4	13	theme	bacterial	609:617	arg1	pathogens					619:627	numerous bacterial pathogens	600:627	numerous bacterial pathogens	600:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	1	14	theme	specific	174:181	arg1	determinants					190:201	specific glycan determinants	174:201	specific glycan determinants exposed on host cell receptors	174:232	Bacterial infections are frequently based on the binding of lectin-like adhesins to specific glycan determinants exposed on host cell receptors.					
31964828	1	15	theme	adhesins	162:169	arg1	binding					139:145	the binding	135:145	the binding of lectin-like adhesins to specific glycan determinants exposed on host cell receptors	135:232	Bacterial infections are frequently based on the binding of lectin-like adhesins to specific glycan determinants exposed on host cell receptors.					
31964828	6	16	theme	receptor	980:987	arg1	recognition					989:999	receptor recognition	980:999	receptor recognition	980:999	Corroborating the inhibitory role of fucosylation on receptor recognition, adhesion of the meningococcus on nonhuman cells expressing human CD147 required prior defucosylation.					
31964828	6	17	theme	inhibitory	945:954	arg1	role					956:959	the inhibitory role	941:959	the inhibitory role of fucosylation on receptor recognition	941:999	Corroborating the inhibitory role of fucosylation on receptor recognition, adhesion of the meningococcus on nonhuman cells expressing human CD147 required prior defucosylation.					
31964828	3	18	theme	carbohydrates	420:432	arg1	diversity					407:415	the wide structural diversity	387:415	the wide structural diversity of carbohydrates	387:432	However, the wide structural diversity of carbohydrates hampers the characterization of specific glycan determinants.					
31964828	4	19	from	present	589:595	arg1	pathogens					619:627	numerous bacterial pathogens	600:627	numerous bacterial pathogens	600:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	6	20	theme	prior	1082:1086	arg1	defucosylation					1088:1101	prior defucosylation	1082:1101	prior defucosylation	1082:1101	Corroborating the inhibitory role of fucosylation on receptor recognition, adhesion of the meningococcus on nonhuman cells expressing human CD147 required prior defucosylation.					
31964828	7	21	theme	receptor	1163:1170	arg1	recognition					1172:1182	the selective receptor recognition	1149:1182	the selective receptor recognition by meningococcal Tfp	1149:1203	These findings reveal the molecular basis of the selective receptor recognition by meningococcal Tfp and thereby, identify a potential antibacterial target.					
31964828	3	22	theme	specific	466:473	arg1	determinants					482:493	specific glycan determinants	466:493	specific glycan determinants	466:493	However, the wide structural diversity of carbohydrates hampers the characterization of specific glycan determinants.					
31964828	0	23	theme	Receptor	0:7	arg1	recognition					9:19	Receptor recognition	0:19	Receptor recognition by meningococcal type IV pili	0:49	Receptor recognition by meningococcal type IV pili relies on a specific complex N-glycan.					
31964828	4	24	theme	adhesive	573:580	arg1	pili					555:558	type IV pili	547:558	type IV pili (Tfp)	547:564	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	24	theme	adhesive	573:580	arg1	factor					582:587	a key adhesive factor	567:587	a key adhesive factor present in numerous bacterial pathogens	567:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	5	25	theme	N-glycan	889:896	arg1	derivatives					869:879	fucosylated derivatives	857:879	fucosylated derivatives of this N-glycan	857:896	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	3	26	theme	glycan	475:480	arg1	determinants					482:493	specific glycan determinants	466:493	specific glycan determinants	466:493	However, the wide structural diversity of carbohydrates hampers the characterization of specific glycan determinants.					
31964828	0	27	theme	meningococcal	24:36	arg1	pili					46:49	meningococcal type IV pili	24:49	meningococcal type IV pili	24:49	Receptor recognition by meningococcal type IV pili relies on a specific complex N-glycan.					
31964828	4	28	theme	key	569:571	arg1	pili					555:558	type IV pili	547:558	type IV pili (Tfp)	547:564	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	28	theme	key	569:571	arg1	factor					582:587	a key adhesive factor	567:587	a key adhesive factor present in numerous bacterial pathogens	567:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	7	29	theme	meningococcal	1187:1199	arg1	Tfp					1201:1203	meningococcal Tfp	1187:1203	meningococcal Tfp	1187:1203	These findings reveal the molecular basis of the selective receptor recognition by meningococcal Tfp and thereby, identify a potential antibacterial target.					
31964828	1	30	theme	Bacterial	90:98	arg1	infections					100:109	Bacterial infections	90:109	Bacterial infections	90:109	Bacterial infections are frequently based on the binding of lectin-like adhesins to specific glycan determinants exposed on host cell receptors.					
31964828	5	31	theme	bacterial	907:915	arg1	adhesion					917:924	bacterial adhesion	907:924	bacterial adhesion	907:924	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	4	32	theme	present	589:595	arg1	pili					555:558	type IV pili	547:558	type IV pili (Tfp)	547:564	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	32	theme	present	589:595	arg1	factor					582:587	a key adhesive factor	567:587	a key adhesive factor present in numerous bacterial pathogens	567:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	5	33	theme	triantennary	737:748	arg1	N-glycan					797:804	a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan	735:804	a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin	735:848	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	6	34	theme	fucosylation	964:975	arg1	role					956:959	the inhibitory role	941:959	the inhibitory role of fucosylation on receptor recognition	941:999	Corroborating the inhibitory role of fucosylation on receptor recognition, adhesion of the meningococcus on nonhuman cells expressing human CD147 required prior defucosylation.					
31964828	2	35	theme	host	317:320	arg1	tissues					322:328	particular host tissues	306:328	particular host tissues	306:328	These interactions confer species-specific recognition and tropism for particular host tissues and represent attractive antibacterial targets.					
31964828	0	36	theme	IV	43:44	arg1	pili					46:49	meningococcal type IV pili	24:49	meningococcal type IV pili	24:49	Receptor recognition by meningococcal type IV pili relies on a specific complex N-glycan.					
31964828	6	37	theme	human	1061:1065	arg1	CD147					1067:1071	human CD147	1061:1071	human CD147	1061:1071	Corroborating the inhibitory role of fucosylation on receptor recognition, adhesion of the meningococcus on nonhuman cells expressing human CD147 required prior defucosylation.					
31964828	5	38	theme	sialylated	750:759	arg1	N-glycan					797:804	a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan	735:804	a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin	735:848	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	1	39	theme	glycan	183:188	arg1	determinants					190:201	specific glycan determinants	174:201	specific glycan determinants exposed on host cell receptors	174:232	Bacterial infections are frequently based on the binding of lectin-like adhesins to specific glycan determinants exposed on host cell receptors.					
31964828	0	40	theme	type	38:41	arg1	pili					46:49	meningococcal type IV pili	24:49	meningococcal type IV pili	24:49	Receptor recognition by meningococcal type IV pili relies on a specific complex N-glycan.					
31964828	5	41	theme	poly-N-acetyllactosamine-containing	761:795	arg1	N-glycan					797:804	a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan	735:804	a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin	735:848	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	3	42	theme	structural	396:405	arg1	diversity					407:415	the wide structural diversity	387:415	the wide structural diversity of carbohydrates	387:432	However, the wide structural diversity of carbohydrates hampers the characterization of specific glycan determinants.					
31964828	7	43	theme	molecular	1130:1138	arg1	basis					1140:1144	the molecular basis	1126:1144	the molecular basis of the selective receptor recognition by meningococcal Tfp	1126:1203	These findings reveal the molecular basis of the selective receptor recognition by meningococcal Tfp and thereby, identify a potential antibacterial target.					
31964828	1	44	theme	host	214:217	arg1	receptors					224:232	host cell receptors	214:232	host cell receptors	214:232	Bacterial infections are frequently based on the binding of lectin-like adhesins to specific glycan determinants exposed on host cell receptors.					
31964828	7	45	theme	recognition	1172:1182	arg1	basis					1140:1144	the molecular basis	1126:1144	the molecular basis of the selective receptor recognition by meningococcal Tfp	1126:1203	These findings reveal the molecular basis of the selective receptor recognition by meningococcal Tfp and thereby, identify a potential antibacterial target.					
31964828	5	46	theme	meningococcal	694:706	arg1	Tfp					708:710	meningococcal Tfp	694:710	meningococcal Tfp	694:710	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	3	47	theme	determinants	482:493	arg1	characterization					446:461	the characterization	442:461	the characterization of specific glycan determinants	442:493	However, the wide structural diversity of carbohydrates hampers the characterization of specific glycan determinants.					
31964828	6	48	theme	nonhuman	1035:1042	arg1	cells					1044:1048	nonhuman cells	1035:1048	nonhuman cells expressing human CD147	1035:1071	Corroborating the inhibitory role of fucosylation on receptor recognition, adhesion of the meningococcus on nonhuman cells expressing human CD147 required prior defucosylation.					
31964828	6	49	theme	meningococcus	1018:1030	arg1	adhesion					1002:1009	adhesion	1002:1009	adhesion of the meningococcus on nonhuman cells expressing human CD147	1002:1071	Corroborating the inhibitory role of fucosylation on receptor recognition, adhesion of the meningococcus on nonhuman cells expressing human CD147 required prior defucosylation.					
31964828	6	50	from	adhesion	1002:1009	arg1	cells					1044:1048	nonhuman cells	1035:1048	nonhuman cells expressing human CD147	1035:1071	Corroborating the inhibitory role of fucosylation on receptor recognition, adhesion of the meningococcus on nonhuman cells expressing human CD147 required prior defucosylation.					
31964828	5	51	gly	fucosylated	857:867	arg1	derivatives					869:879	fucosylated derivatives	857:879	fucosylated derivatives of this N-glycan	857:896	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	2	52	theme	antibacterial	355:367	arg1	targets					369:375	attractive antibacterial targets	344:375	attractive antibacterial targets	344:375	These interactions confer species-specific recognition and tropism for particular host tissues and represent attractive antibacterial targets.					
31964828	1	53	theme	cell	219:222	arg1	receptors					224:232	host cell receptors	214:232	host cell receptors	214:232	Bacterial infections are frequently based on the binding of lectin-like adhesins to specific glycan determinants exposed on host cell receptors.					
31964828	4	54	attach	present	589:595	arg1	pathogens					619:627	numerous bacterial pathogens	600:627	numerous bacterial pathogens	600:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	54	attach	present	589:595	arg2	factor					582:587	a key adhesive factor	567:587	a key adhesive factor present in numerous bacterial pathogens	567:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	54	attach	present	589:595	arg2	pili					555:558	type IV pili	547:558	type IV pili (Tfp)	547:564	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	2	55	theme	species-specific	261:276	arg1	recognition					278:288	species-specific recognition	261:288	species-specific recognition	261:288	These interactions confer species-specific recognition and tropism for particular host tissues and represent attractive antibacterial targets.					
31964828	2	56	theme	attractive	344:353	arg1	targets					369:375	attractive antibacterial targets	344:375	attractive antibacterial targets	344:375	These interactions confer species-specific recognition and tropism for particular host tissues and represent attractive antibacterial targets.					
31964828	5	57	theme	human	821:825	arg1	CD147/Basigin					836:848	the human receptor CD147/Basigin	817:848	the human receptor CD147/Basigin	817:848	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	4	58	theme	model	664:668	arg1	organism					670:677	a model organism	662:677	a model organism	662:677	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	58	theme	model	664:668	arg1	meningitidis					646:657	Neisseria meningitidis	636:657	Neisseria meningitidis	636:657	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	5	59	gly	sialylated	750:759	arg1	N-glycan					797:804	a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan	735:804	a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin	735:848	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	5	60	theme	receptor	827:834	arg1	CD147/Basigin					836:848	the human receptor CD147/Basigin	817:848	the human receptor CD147/Basigin	817:848	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	4	61	theme	IV	552:553	arg1	factor					582:587	a key adhesive factor	567:587	a key adhesive factor present in numerous bacterial pathogens	567:627	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	61	theme	IV	552:553	arg1	Tfp					561:563	Tfp	561:563	Tfp	561:563	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	61	theme	IV	552:553	arg1	pili					555:558	type IV pili	547:558	type IV pili (Tfp)	547:564	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	5	62	theme	fucosylated	857:867	arg1	derivatives					869:879	fucosylated derivatives	857:879	fucosylated derivatives of this N-glycan	857:896	We found that meningococcal Tfp specifically recognize a triantennary sialylated poly-N-acetyllactosamine-containing N-glycan exposed on the human receptor CD147/Basigin, while fucosylated derivatives of this N-glycan impaired bacterial adhesion.					
31964828	6	63	from	role	956:959	arg1	recognition					989:999	receptor recognition	980:999	receptor recognition	980:999	Corroborating the inhibitory role of fucosylation on receptor recognition, adhesion of the meningococcus on nonhuman cells expressing human CD147 required prior defucosylation.					
31964828	4	64	theme	Neisseria	636:644	arg1	organism					670:677	a model organism	662:677	a model organism	662:677	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
31964828	4	64	theme	Neisseria	636:644	arg1	meningitidis					646:657	Neisseria meningitidis	636:657	Neisseria meningitidis	636:657	Here, we characterized the receptor recognition of type IV pili (Tfp), a key adhesive factor present in numerous bacterial pathogens, using Neisseria meningitidis as a model organism.					
34742721	0	0	theme	interaction	84:94	arg1	spectrometry					140:151	hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	72:151	hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	72:151	Glycine additive enhances sensitivity for N- and O-glycan analysis with hydrophilic interaction chromatography-electrospray ionization-mass spectrometry.					
34742721	6	1	theme	labeled	1015:1021	arg1	glycans					1023:1029	different ProcA labeled glycans	999:1029	different ProcA labeled glycans	999:1029	Furthermore, more homogeneous ionization among different ProcA labeled glycans was achieved by using the glycine additive, resulting in more comparable quantitative results relative to fluorescence-based quantification.					
34742721	8	2	theme	HILIC-ESI-MS	1388:1399	arg1	analysis					1401:1408	HILIC-ESI-MS analysis	1388:1408	HILIC-ESI-MS analysis	1388:1408	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	6	3	theme	comparable	1093:1102	arg1	results					1117:1123	more comparable quantitative results	1088:1123	more comparable quantitative results relative to fluorescence-based quantification	1088:1169	Furthermore, more homogeneous ionization among different ProcA labeled glycans was achieved by using the glycine additive, resulting in more comparable quantitative results relative to fluorescence-based quantification.					
34742721	0	4	theme	hydrophilic	72:82	arg1	spectrometry					140:151	hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	72:151	hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	72:151	Glycine additive enhances sensitivity for N- and O-glycan analysis with hydrophilic interaction chromatography-electrospray ionization-mass spectrometry.					
34742721	6	5	theme	ProcA	1009:1013	arg1	glycans					1023:1029	different ProcA labeled glycans	999:1029	different ProcA labeled glycans	999:1029	Furthermore, more homogeneous ionization among different ProcA labeled glycans was achieved by using the glycine additive, resulting in more comparable quantitative results relative to fluorescence-based quantification.					
34742721	5	6	theme	spectral	860:867	arg1	quality					869:875	tandem MS spectral quality	850:875	tandem MS spectral quality of various N- and O-glycans	850:903	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	0	7	theme	ionization-mass	124:138	arg1	spectrometry					140:151	hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	72:151	hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	72:151	Glycine additive enhances sensitivity for N- and O-glycan analysis with hydrophilic interaction chromatography-electrospray ionization-mass spectrometry.					
34742721	4	8	theme	MS	585:586	arg1	response					588:595	MS response	585:595	MS response of procainamide (ProcA) labeled glycans for 2- to	585:645	In this study, we presented a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS.					
34742721	7	9	theme	ProcA	1236:1240	arg1	glycans					1250:1256	ProcA labeled glycans	1236:1256	ProcA labeled glycans	1236:1256	We demonstrated that ammonium formate caused ion suppression to ProcA labeled glycans, which were likely mitigated by glycine with enhanced ESI ionization.					
34742721	2	10	theme	electrospray	364:375	arg1	HILIC-ESI-MS					407:418	HILIC-ESI-MS	407:418	HILIC-ESI-MS	407:418	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	2	10	theme	electrospray	364:375	arg1	spectrometry					393:404	electrospray ionization mass spectrometry	364:404	electrospray ionization mass spectrometry (HILIC-ESI-MS)	364:419	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	3	11	theme	MS	446:447	arg1	sensitivity					431:441	The high sensitivity	422:441	The high sensitivity of MS	422:447	The high sensitivity of MS is crucial for detecting low-abundance glycans and elucidating their structures.					
34742721	3	11	theme	MS	446:447	arg1	crucial					452:458	crucial	452:458	crucial	452:458	The high sensitivity of MS is crucial for detecting low-abundance glycans and elucidating their structures.					
34742721	7	12	theme	labeled	1242:1248	arg1	glycans					1250:1256	ProcA labeled glycans	1236:1256	ProcA labeled glycans	1236:1256	We demonstrated that ammonium formate caused ion suppression to ProcA labeled glycans, which were likely mitigated by glycine with enhanced ESI ionization.					
34742721	8	13	theme	future	1478:1483	arg1	profiling					1485:1493	future profiling	1478:1493	future profiling	1478:1493	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	6	14	theme	quantitative	1104:1115	arg1	results					1117:1123	more comparable quantitative results	1088:1123	more comparable quantitative results relative to fluorescence-based quantification	1088:1169	Furthermore, more homogeneous ionization among different ProcA labeled glycans was achieved by using the glycine additive, resulting in more comparable quantitative results relative to fluorescence-based quantification.					
34742721	3	15	theme	high	426:429	arg1	sensitivity					431:441	The high sensitivity	422:441	The high sensitivity of MS	422:447	The high sensitivity of MS is crucial for detecting low-abundance glycans and elucidating their structures.					
34742721	3	15	theme	high	426:429	arg1	crucial					452:458	crucial	452:458	crucial	452:458	The high sensitivity of MS is crucial for detecting low-abundance glycans and elucidating their structures.					
34742721	0	16	with	analysis	58:65	arg1	spectrometry					140:151	hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	72:151	hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	72:151	Glycine additive enhances sensitivity for N- and O-glycan analysis with hydrophilic interaction chromatography-electrospray ionization-mass spectrometry.					
34742721	1	17	theme	biotherapeutic	214:227	arg1	development					229:239	biotherapeutic development	214:239	biotherapeutic development	214:239	Glycosylation is critical for many biological processes and biotherapeutic development.					
34742721	5	18	theme	signal-to-noise	813:827	arg1	ratios					829:834	signal-to-noise ratios	813:834	signal-to-noise ratios	813:834	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	6	19	theme	homogeneous	970:980	arg1	ionization					982:991	more homogeneous ionization	965:991	more homogeneous ionization among different ProcA labeled glycans	965:1029	Furthermore, more homogeneous ionization among different ProcA labeled glycans was achieved by using the glycine additive, resulting in more comparable quantitative results relative to fluorescence-based quantification.					
34742721	5	20	theme	tandem	850:855	arg1	MS					857:858	tandem MS	850:858	tandem MS spectral quality of various N- and O-glycans	850:903	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	7	21	theme	enhanced	1303:1310	arg1	ionization					1316:1325	enhanced ESI ionization	1303:1325	enhanced ESI ionization	1303:1325	We demonstrated that ammonium formate caused ion suppression to ProcA labeled glycans, which were likely mitigated by glycine with enhanced ESI ionization.					
34742721	7	22	theme	ESI	1312:1314	arg1	ionization					1316:1325	enhanced ESI ionization	1303:1325	enhanced ESI ionization	1303:1325	We demonstrated that ammonium formate caused ion suppression to ProcA labeled glycans, which were likely mitigated by glycine with enhanced ESI ionization.					
34742721	0	23	theme	additive	8:15	arg1	Glycine					0:6	Glycine additive	0:15	Glycine additive	0:15	Glycine additive enhances sensitivity for N- and O-glycan analysis with hydrophilic interaction chromatography-electrospray ionization-mass spectrometry.					
34742721	4	24	theme	simple	560:565	arg1	solution					567:574	a simple solution	558:574	a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS	558:735	In this study, we presented a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS.					
34742721	5	25	theme	various	880:886	arg1	N-					888:889	various N-	880:889	various N-	880:889	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	4	26	theme	labeled	621:627	arg1	glycans					629:635	procainamide (ProcA) labeled glycans	600:635	procainamide (ProcA) labeled glycans for 2-	600:642	In this study, we presented a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS.					
34742721	7	27	theme	ion	1217:1219	arg1	suppression					1221:1231	ion suppression	1217:1231	ion suppression	1217:1231	We demonstrated that ammonium formate caused ion suppression to ProcA labeled glycans, which were likely mitigated by glycine with enhanced ESI ionization.					
34742721	8	28	theme	glycans	1515:1521	arg1	quantitation					1499:1510	quantitation	1499:1510	quantitation	1499:1510	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	8	28	theme	glycans	1515:1521	arg1	profiling					1485:1493	future profiling	1478:1493	future profiling	1478:1493	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	8	29	theme	mobile	1367:1372	arg1	phases					1374:1379	mobile phases	1367:1379	mobile phases	1367:1379	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	5	30	theme	N-	888:889	arg1	quality					869:875	tandem MS spectral quality	850:875	tandem MS spectral quality of various N- and O-glycans	850:903	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	6	31	theme	additive	1065:1072	arg1	glycine					1057:1063	the glycine additive	1053:1072	the glycine additive	1053:1072	Furthermore, more homogeneous ionization among different ProcA labeled glycans was achieved by using the glycine additive, resulting in more comparable quantitative results relative to fluorescence-based quantification.					
34742721	5	32	theme	charge	769:774	arg1	states					776:781	charge states	769:781	charge states	769:781	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	2	33	theme	mass	388:391	arg1	HILIC-ESI-MS					407:418	HILIC-ESI-MS	407:418	HILIC-ESI-MS	407:418	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	2	33	theme	mass	388:391	arg1	spectrometry					393:404	electrospray ionization mass spectrometry	364:404	electrospray ionization mass spectrometry (HILIC-ESI-MS)	364:419	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	4	34	theme	ammonium	689:696	arg1	phases					713:718	ammonium formate mobile phases	689:718	ammonium formate mobile phases for HILIC-ESI-MS	689:735	In this study, we presented a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS.					
34742721	8	35	attach	released	1523:1530	arg1	O-glycoproteins					1544:1558	O-glycoproteins	1544:1558	O-glycoproteins	1544:1558	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	8	35	attach	released	1523:1530	arg2	glycans					1515:1521	glycans	1515:1521	glycans released from N- and O-glycoproteins	1515:1558	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	8	35	attach	released	1523:1530	arg1	N-					1537:1538	N-	1537:1538	N-	1537:1538	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	2	36	theme	ionization	377:386	arg1	HILIC-ESI-MS					407:418	HILIC-ESI-MS	407:418	HILIC-ESI-MS	407:418	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	2	36	theme	ionization	377:386	arg1	spectrometry					393:404	electrospray ionization mass spectrometry	364:404	electrospray ionization mass spectrometry (HILIC-ESI-MS)	364:419	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	4	37	attach	presented	548:556	arg2	we					545:546	we	545:546	we	545:546	In this study, we presented a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS.					
34742721	4	37	attach	presented	548:556	arg1	study					538:542	this study	533:542	this study	533:542	In this study, we presented a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS.					
34742721	0	38	theme	N-	42:43	arg1	analysis					58:65	N- and O-glycan analysis	42:65	N- and O-glycan analysis with hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	42:151	Glycine additive enhances sensitivity for N- and O-glycan analysis with hydrophilic interaction chromatography-electrospray ionization-mass spectrometry.					
34742721	4	39	theme	glycans	629:635	arg1	response					588:595	MS response	585:595	MS response of procainamide (ProcA) labeled glycans for 2- to	585:645	In this study, we presented a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS.					
34742721	2	40	theme	released	292:299	arg1	glycans					301:307	released glycans	292:307	released glycans	292:307	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	4	41	theme	mobile	706:711	arg1	phases					713:718	ammonium formate mobile phases	689:718	ammonium formate mobile phases for HILIC-ESI-MS	689:735	In this study, we presented a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS.					
34742721	5	42	theme	MS	857:858	arg1	quality					869:875	tandem MS spectral quality	850:875	tandem MS spectral quality of various N- and O-glycans	850:903	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	4	43	theme	formate	698:704	arg1	phases					713:718	ammonium formate mobile phases	689:718	ammonium formate mobile phases for HILIC-ESI-MS	689:735	In this study, we presented a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS.					
34742721	0	44	theme	O-glycan	49:56	arg1	analysis					58:65	N- and O-glycan analysis	42:65	N- and O-glycan analysis with hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	42:151	Glycine additive enhances sensitivity for N- and O-glycan analysis with hydrophilic interaction chromatography-electrospray ionization-mass spectrometry.					
34742721	5	45	theme	ion	793:795	arg1	intensities					797:807	ion intensities	793:807	ion intensities	793:807	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	6	46	theme	fluorescence-based	1137:1154	arg1	quantification					1156:1169	fluorescence-based quantification	1137:1169	fluorescence-based quantification	1137:1169	Furthermore, more homogeneous ionization among different ProcA labeled glycans was achieved by using the glycine additive, resulting in more comparable quantitative results relative to fluorescence-based quantification.					
34742721	8	47	theme	MS	1433:1434	arg1	sensitivity					1446:1456	MS detection sensitivity	1433:1456	MS detection sensitivity	1433:1456	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	6	48	theme	different	999:1007	arg1	glycans					1023:1029	different ProcA labeled glycans	999:1029	different ProcA labeled glycans	999:1029	Furthermore, more homogeneous ionization among different ProcA labeled glycans was achieved by using the glycine additive, resulting in more comparable quantitative results relative to fluorescence-based quantification.					
34742721	7	49	theme	ammonium	1193:1200	arg1	formate					1202:1208	ammonium formate	1193:1208	ammonium formate	1193:1208	We demonstrated that ammonium formate caused ion suppression to ProcA labeled glycans, which were likely mitigated by glycine with enhanced ESI ionization.					
34742721	2	50	theme	powerful	258:265	arg1	approaches					267:276	the most powerful approaches	249:276	the most powerful approaches for analyzing released glycans	249:307	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	2	51	theme	approaches	267:276	arg1	approaches					267:276	the most powerful approaches	249:276	the most powerful approaches for analyzing released glycans	249:307	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	2	51	theme	approaches	267:276	arg1	One					242:244	One	242:244	One	242:244	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	8	52	theme	simple	1337:1342	arg1	addition					1344:1351	simple addition	1337:1351	simple addition of glycine to mobile phases during HILIC-ESI-MS analysis	1337:1408	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	5	53	theme	additive	750:757	arg1	glycine					742:748	The glycine additive	738:757	The glycine additive	738:757	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	2	54	theme	interaction	324:334	arg1	chromatography					336:349	hydrophilic interaction chromatography	312:349	hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS)	312:419	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	8	55	gly	O-glycoproteins	1544:1558	arg1	O-glycoproteins					1544:1558	O-glycoproteins	1544:1558	O-glycoproteins	1544:1558	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	2	56	theme	hydrophilic	312:322	arg1	chromatography					336:349	hydrophilic interaction chromatography	312:349	hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS)	312:419	One of the most powerful approaches for analyzing released glycans is hydrophilic interaction chromatography coupled with electrospray ionization mass spectrometry (HILIC-ESI-MS).					
34742721	8	57	theme	detection	1436:1444	arg1	sensitivity					1446:1456	MS detection sensitivity	1433:1456	MS detection sensitivity	1433:1456	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	6	58	theme	relative	1125:1132	arg1	results					1117:1123	more comparable quantitative results	1088:1123	more comparable quantitative results relative to fluorescence-based quantification	1088:1169	Furthermore, more homogeneous ionization among different ProcA labeled glycans was achieved by using the glycine additive, resulting in more comparable quantitative results relative to fluorescence-based quantification.					
34742721	5	59	theme	chromatographic	923:937	arg1	performance					939:949	chromatographic performance	923:949	chromatographic performance	923:949	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	5	60	theme	O-glycans	895:903	arg1	quality					869:875	tandem MS spectral quality	850:875	tandem MS spectral quality of various N- and O-glycans	850:903	The glycine additive increased charge states, enhanced ion intensities and signal-to-noise ratios, and improved tandem MS spectral quality of various N- and O-glycans without affecting chromatographic performance.					
34742721	1	61	theme	many	184:187	arg1	processes					200:208	many biological processes	184:208	many biological processes	184:208	Glycosylation is critical for many biological processes and biotherapeutic development.					
34742721	8	62	theme	glycine	1356:1362	arg1	addition					1344:1351	simple addition	1337:1351	simple addition of glycine to mobile phases during HILIC-ESI-MS analysis	1337:1408	Overall, simple addition of glycine to mobile phases during HILIC-ESI-MS analysis significantly improves MS detection sensitivity and will facilitate future profiling and quantitation of glycans released from N- and O-glycoproteins.					
34742721	3	63	theme	low-abundance	474:486	arg1	glycans					488:494	low-abundance glycans	474:494	low-abundance glycans	474:494	The high sensitivity of MS is crucial for detecting low-abundance glycans and elucidating their structures.					
34742721	4	64	theme	1 mM	673:676	arg1	glycine					678:684	1 mM glycine	673:684	1 mM glycine	673:684	In this study, we presented a simple solution to boost MS response of procainamide (ProcA) labeled glycans for 2- to over 60-fold by including 1 mM glycine in ammonium formate mobile phases for HILIC-ESI-MS.					
34742721	1	65	theme	biological	189:198	arg1	processes					200:208	many biological processes	184:208	many biological processes	184:208	Glycosylation is critical for many biological processes and biotherapeutic development.					
34742721	0	66	theme	chromatography-electrospray	96:122	arg1	spectrometry					140:151	hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	72:151	hydrophilic interaction chromatography-electrospray ionization-mass spectrometry	72:151	Glycine additive enhances sensitivity for N- and O-glycan analysis with hydrophilic interaction chromatography-electrospray ionization-mass spectrometry.					
33262351	4	0	theme	structures	634:643	arg1	association					706:716	the association	702:716	the association of fucosylation with mesenchymal subtype	702:757	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	4	0	theme	structures	634:643	arg1	example					679:685	one example	675:685	one example	675:685	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	7	1	theme	glycoprotein-modifying	1260:1281	arg1	glycans					1283:1289	the glycoprotein-modifying glycans	1256:1289	the glycoprotein-modifying glycans	1256:1289	The results show that glycoprotein levels are mainly controlled by the expression of their individual proteins, and, furthermore, that the glycoprotein-modifying glycans correspond to the protein levels of glycosylation enzymes.					
33262351	4	2	theme	tumor	649:653	arg1	subtypes					665:672	tumor molecular subtypes	649:672	tumor molecular subtypes	649:672	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	2	3	gly	heterogeneity	304:316	arg1	HGSC					358:361	HGSC	358:361	HGSC	358:361	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	2	3	gly	heterogeneity	304:316	arg1	carcinoma					347:355	high-grade serous ovarian carcinoma	321:355	high-grade serous ovarian carcinoma (HGSC)	321:362	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	1	4	theme	translational	189:201	arg1	features					237:244	genomic, transcriptional, translational, and post-translational molecular features	163:244	genomic, transcriptional, translational, and post-translational molecular features	163:244	Inter-tumor heterogeneity is a result of genomic, transcriptional, translational, and post-translational molecular features.					
33262351	7	5	gly	glycoprotein	1143:1154	arg1	glycoprotein					1143:1154	glycoprotein levels	1143:1161	glycoprotein levels	1143:1161	The results show that glycoprotein levels are mainly controlled by the expression of their individual proteins, and, furthermore, that the glycoprotein-modifying glycans correspond to the protein levels of glycosylation enzymes.					
33262351	7	6	theme	glycoprotein	1143:1154	arg1	levels					1156:1161	glycoprotein levels	1143:1161	glycoprotein levels	1143:1161	The results show that glycoprotein levels are mainly controlled by the expression of their individual proteins, and, furthermore, that the glycoprotein-modifying glycans correspond to the protein levels of glycosylation enzymes.					
33262351	2	7	theme	carcinoma	347:355	arg1	heterogeneity					304:316	the heterogeneity	300:316	the heterogeneity of high-grade serous ovarian carcinoma (HGSC)	300:362	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	5	8	theme	survival	768:775	arg1	analysis					777:784	Further survival analysis	760:784	Further survival analysis	760:784	Further survival analysis reveals that intact glycopeptide signatures of mesenchymal subtype are associated with a poor clinical outcome of HGSC.					
33262351	2	9	theme	serous	332:337	arg1	HGSC					358:361	HGSC	358:361	HGSC	358:361	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	2	9	theme	serous	332:337	arg1	carcinoma					347:355	high-grade serous ovarian carcinoma	321:355	high-grade serous ovarian carcinoma (HGSC)	321:362	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	1	10	theme	molecular	227:235	arg1	features					237:244	genomic, transcriptional, translational, and post-translational molecular features	163:244	genomic, transcriptional, translational, and post-translational molecular features	163:244	Inter-tumor heterogeneity is a result of genomic, transcriptional, translational, and post-translational molecular features.					
33262351	7	11	theme	proteins	1223:1230	arg1	expression					1192:1201	the expression	1188:1201	the expression of their individual proteins	1188:1230	The results show that glycoprotein levels are mainly controlled by the expression of their individual proteins, and, furthermore, that the glycoprotein-modifying glycans correspond to the protein levels of glycosylation enzymes.					
33262351	9	12	theme	precision	1576:1584	arg1	medicine					1586:1593	precision medicine	1576:1593	precision medicine	1576:1593	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	6	13	theme	intact	979:984	arg1	glycopeptides					986:998	intact glycopeptides	979:998	intact glycopeptides	979:998	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	2	14	theme	glycosylation	283:295	arg1	roles					266:270	the roles	262:270	the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC)	262:362	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	6	15	theme	glycoprotein	1071:1082	arg1	pathways					1097:1104	glycoprotein biosynthesis pathways	1071:1104	glycoprotein biosynthesis pathways in each tumor	1071:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	4	16	theme	mesenchymal	739:749	arg1	subtype					751:757	mesenchymal subtype	739:757	mesenchymal subtype	739:757	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	5	17	theme	mesenchymal	833:843	arg1	subtype					845:851	mesenchymal subtype	833:851	mesenchymal subtype	833:851	Further survival analysis reveals that intact glycopeptide signatures of mesenchymal subtype are associated with a poor clinical outcome of HGSC.					
33262351	1	18	theme	genomic	163:169	arg1	features					237:244	genomic, transcriptional, translational, and post-translational molecular features	163:244	genomic, transcriptional, translational, and post-translational molecular features	163:244	Inter-tumor heterogeneity is a result of genomic, transcriptional, translational, and post-translational molecular features.					
33262351	0	19	theme	high-grade	88:97	arg1	cancer					114:119	high-grade serous ovarian cancer	88:119	high-grade serous ovarian cancer	88:119	Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer.					
33262351	1	20	theme	transcriptional	172:186	arg1	features					237:244	genomic, transcriptional, translational, and post-translational molecular features	163:244	genomic, transcriptional, translational, and post-translational molecular features	163:244	Inter-tumor heterogeneity is a result of genomic, transcriptional, translational, and post-translational molecular features.					
33262351	6	21	theme	glycosylation	1037:1049	arg1	enzymes					1051:1057	glycosylation enzymes	1037:1057	glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor	1037:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	3	22	gly	glycopeptides	566:578	arg2	glycopeptides					566:578	intact glycopeptides	559:578	intact glycopeptides	559:578	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	6	23	gly	glycosites	963:972	arg2	glycosites					963:972	glycosites	963:972	glycosites	963:972	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	0	24	theme	ovarian	106:112	arg1	cancer					114:119	high-grade serous ovarian cancer	88:119	high-grade serous ovarian cancer	88:119	Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer.					
33262351	9	25	from	understanding	1442:1454	arg1	subtypes					1527:1534	different subtypes	1517:1534	different subtypes of HGSC	1517:1542	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	2	26	theme	TCGA	439:442	arg1	tissues					449:455	119 TCGA HGSC tissues	435:455	119 TCGA HGSC tissues	435:455	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	2	27	from	roles	266:270	arg1	heterogeneity					304:316	the heterogeneity	300:316	the heterogeneity of high-grade serous ovarian carcinoma (HGSC)	300:362	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	0	28	theme	Glycoproteomics-based	0:20	arg1	signatures					22:31	Glycoproteomics-based signatures	0:31	Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer.	0:120	Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer.					
33262351	5	29	theme	HGSC	900:903	arg1	outcome					889:895	a poor clinical outcome	873:895	a poor clinical outcome of HGSC	873:903	Further survival analysis reveals that intact glycopeptide signatures of mesenchymal subtype are associated with a poor clinical outcome of HGSC.					
33262351	1	30	theme	Inter-tumor	122:132	arg1	heterogeneity					134:146	Inter-tumor heterogeneity	122:146	Inter-tumor heterogeneity	122:146	Inter-tumor heterogeneity is a result of genomic, transcriptional, translational, and post-translational molecular features.					
33262351	1	30	theme	Inter-tumor	122:132	arg1	result					153:158	a result	151:158	a result of genomic, transcriptional, translational, and post-translational molecular features	151:244	Inter-tumor heterogeneity is a result of genomic, transcriptional, translational, and post-translational molecular features.					
33262351	2	31	theme	tissues	449:455	arg1	characterization					415:430	mass spectrometry-based glycoproteomic characterization	376:430	mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues	376:455	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	3	32	theme	profiles	500:507	arg1	analysis					466:473	Cluster analysis	458:473	Cluster analysis of intact glycoproteomic profiles	458:507	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	0	33	theme	subtyping	43:51	arg1	prediction					74:83	tumor subtyping and clinical outcome prediction	37:83	tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer	37:119	Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer.					
33262351	9	34	theme	Deeper	1435:1440	arg1	understanding					1442:1454	Deeper understanding	1435:1454	Deeper understanding of the glycosylation process	1435:1483	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	7	35	theme	individual	1212:1221	arg1	proteins					1223:1230	their individual proteins	1206:1230	their individual proteins	1206:1230	The results show that glycoprotein levels are mainly controlled by the expression of their individual proteins, and, furthermore, that the glycoprotein-modifying glycans correspond to the protein levels of glycosylation enzymes.					
33262351	3	36	theme	intact	478:483	arg1	profiles					500:507	intact glycoproteomic profiles	478:507	intact glycoproteomic profiles	478:507	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	5	37	theme	poor	875:878	arg1	outcome					889:895	a poor clinical outcome	873:895	a poor clinical outcome of HGSC	873:903	Further survival analysis reveals that intact glycopeptide signatures of mesenchymal subtype are associated with a poor clinical outcome of HGSC.					
33262351	3	38	theme	tumor	528:532	arg1	clusters					534:541	3 major tumor clusters	520:541	3 major tumor clusters	520:541	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	9	39	theme	glycosylation	1463:1475	arg1	process					1477:1483	the glycosylation process	1459:1483	the glycosylation process	1459:1483	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	7	40	theme	enzymes	1341:1347	arg1	levels					1317:1322	the protein levels	1305:1322	the protein levels of glycosylation enzymes	1305:1347	The results show that glycoprotein levels are mainly controlled by the expression of their individual proteins, and, furthermore, that the glycoprotein-modifying glycans correspond to the protein levels of glycosylation enzymes.					
33262351	4	41	theme	molecular	655:663	arg1	subtypes					665:672	tumor molecular subtypes	649:672	tumor molecular subtypes	649:672	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	3	42	theme	intact	559:564	arg1	glycopeptides					566:578	intact glycopeptides	559:578	intact glycopeptides	559:578	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	9	43	theme	HGSC	1539:1542	arg1	subtypes					1527:1534	different subtypes	1517:1534	different subtypes of HGSC	1517:1542	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	5	44	theme	subtype	845:851	arg1	signatures					819:828	intact glycopeptide signatures	799:828	intact glycopeptide signatures of mesenchymal subtype	799:851	Further survival analysis reveals that intact glycopeptide signatures of mesenchymal subtype are associated with a poor clinical outcome of HGSC.					
33262351	2	45	theme	mass	376:379	arg1	characterization					415:430	mass spectrometry-based glycoproteomic characterization	376:430	mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues	376:455	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	6	46	theme	glycosites	963:972	arg1	levels					1027:1032	the expression levels	1012:1032	the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor	1012:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	6	46	theme	glycosites	963:972	arg1	expression					932:941	the expression	928:941	the expression of mRNAs, proteins, glycosites, and intact glycopeptides	928:998	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	7	47	theme	protein	1309:1315	arg1	levels					1317:1322	the protein levels	1305:1322	the protein levels of glycosylation enzymes	1305:1347	The results show that glycoprotein levels are mainly controlled by the expression of their individual proteins, and, furthermore, that the glycoprotein-modifying glycans correspond to the protein levels of glycosylation enzymes.					
33262351	4	48	theme	N-glycan	625:632	arg1	structures					634:643	N-glycan structures	625:643	N-glycan structures	625:643	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	2	49	theme	high-grade	321:330	arg1	HGSC					358:361	HGSC	358:361	HGSC	358:361	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	2	49	theme	high-grade	321:330	arg1	carcinoma					347:355	high-grade serous ovarian carcinoma	321:355	high-grade serous ovarian carcinoma (HGSC)	321:362	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	9	50	theme	different	1517:1525	arg1	subtypes					1527:1534	different subtypes	1517:1534	different subtypes of HGSC	1517:1542	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	6	51	theme	proteins	953:960	arg1	levels					1027:1032	the expression levels	1012:1032	the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor	1012:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	6	51	theme	proteins	953:960	arg1	expression					932:941	the expression	928:941	the expression of mRNAs, proteins, glycosites, and intact glycopeptides	928:998	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	6	52	gly	glycopeptides	986:998	arg2	glycopeptides					986:998	intact glycopeptides	979:998	intact glycopeptides	979:998	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	4	53	theme	fucosylation	721:732	arg1	association					706:716	the association	702:716	the association of fucosylation with mesenchymal subtype	702:757	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	4	53	theme	fucosylation	721:732	arg1	example					679:685	one example	675:685	one example	675:685	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	0	54	theme	clinical	57:64	arg1	prediction					74:83	tumor subtyping and clinical outcome prediction	37:83	tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer	37:119	Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer.					
33262351	6	55	theme	mRNAs	946:950	arg1	levels					1027:1032	the expression levels	1012:1032	the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor	1012:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	6	55	theme	mRNAs	946:950	arg1	expression					932:941	the expression	928:941	the expression of mRNAs, proteins, glycosites, and intact glycopeptides	928:998	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	2	56	gly	glycosylation	283:295	arg1	HGSC					358:361	HGSC	358:361	HGSC	358:361	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	2	56	gly	glycosylation	283:295	arg1	carcinoma					347:355	high-grade serous ovarian carcinoma	321:355	high-grade serous ovarian carcinoma (HGSC)	321:362	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	6	57	from	pathways	1097:1104	arg1	tumor					1114:1118	each tumor	1109:1118	each tumor	1109:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	6	58	theme	expression	1016:1025	arg1	levels					1027:1032	the expression levels	1012:1032	the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor	1012:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	5	59	theme	Further	760:766	arg1	analysis					777:784	Further survival analysis	760:784	Further survival analysis	760:784	Further survival analysis reveals that intact glycopeptide signatures of mesenchymal subtype are associated with a poor clinical outcome of HGSC.					
33262351	2	60	theme	ovarian	339:345	arg1	HGSC					358:361	HGSC	358:361	HGSC	358:361	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	2	60	theme	ovarian	339:345	arg1	carcinoma					347:355	high-grade serous ovarian carcinoma	321:355	high-grade serous ovarian carcinoma (HGSC)	321:362	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	1	61	theme	post-translational	208:225	arg1	features					237:244	genomic, transcriptional, translational, and post-translational molecular features	163:244	genomic, transcriptional, translational, and post-translational molecular features	163:244	Inter-tumor heterogeneity is a result of genomic, transcriptional, translational, and post-translational molecular features.					
33262351	2	62	theme	glycoproteomic	400:413	arg1	characterization					415:430	mass spectrometry-based glycoproteomic characterization	376:430	mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues	376:455	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	1	63	theme	features	237:244	arg1	heterogeneity					134:146	Inter-tumor heterogeneity	122:146	Inter-tumor heterogeneity	122:146	Inter-tumor heterogeneity is a result of genomic, transcriptional, translational, and post-translational molecular features.					
33262351	1	63	theme	features	237:244	arg1	result					153:158	a result	151:158	a result of genomic, transcriptional, translational, and post-translational molecular features	151:244	Inter-tumor heterogeneity is a result of genomic, transcriptional, translational, and post-translational molecular features.					
33262351	6	64	theme	glycopeptides	986:998	arg1	levels					1027:1032	the expression levels	1012:1032	the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor	1012:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	6	64	theme	glycopeptides	986:998	arg1	expression					932:941	the expression	928:941	the expression of mRNAs, proteins, glycosites, and intact glycopeptides	928:998	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	9	65	theme	tumor-targeted	1599:1612	arg1	therapy					1614:1620	tumor-targeted therapy	1599:1620	tumor-targeted therapy	1599:1620	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	6	66	theme	biosynthesis	1084:1095	arg1	pathways					1097:1104	glycoprotein biosynthesis pathways	1071:1104	glycoprotein biosynthesis pathways in each tumor	1071:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	8	67	theme	tumor	1414:1418	arg1	heterogeneity					1420:1432	the tumor heterogeneity	1410:1432	the tumor heterogeneity	1410:1432	The variation in glycan types further shows coordination to the tumor heterogeneity.					
33262351	8	68	theme	glycan	1367:1372	arg1	types					1374:1378	glycan types	1367:1378	glycan types	1367:1378	The variation in glycan types further shows coordination to the tumor heterogeneity.					
33262351	2	69	theme	protein	275:281	arg1	glycosylation					283:295	protein glycosylation	275:295	protein glycosylation	275:295	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	9	70	theme	important	1556:1564	arg1	clues					1566:1570	important clues	1556:1570	important clues for precision medicine and tumor-targeted therapy	1556:1620	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	5	71	theme	intact	799:804	arg1	signatures					819:828	intact glycopeptide signatures	799:828	intact glycopeptide signatures of mesenchymal subtype	799:851	Further survival analysis reveals that intact glycopeptide signatures of mesenchymal subtype are associated with a poor clinical outcome of HGSC.					
33262351	0	72	theme	serous	99:104	arg1	cancer					114:119	high-grade serous ovarian cancer	88:119	high-grade serous ovarian cancer	88:119	Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer.					
33262351	6	73	theme	enzymes	1051:1057	arg1	levels					1027:1032	the expression levels	1012:1032	the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor	1012:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	6	73	theme	enzymes	1051:1057	arg1	expression					932:941	the expression	928:941	the expression of mRNAs, proteins, glycosites, and intact glycopeptides	928:998	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	0	74	theme	cancer	114:119	arg1	prediction					74:83	tumor subtyping and clinical outcome prediction	37:83	tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer	37:119	Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer.					
33262351	5	75	theme	glycopeptide	806:817	arg1	signatures					819:828	intact glycopeptide signatures	799:828	intact glycopeptide signatures of mesenchymal subtype	799:851	Further survival analysis reveals that intact glycopeptide signatures of mesenchymal subtype are associated with a poor clinical outcome of HGSC.					
33262351	8	76	from	variation	1354:1362	arg1	types					1374:1378	glycan types	1367:1378	glycan types	1367:1378	The variation in glycan types further shows coordination to the tumor heterogeneity.					
33262351	3	77	theme	glycoproteomic	485:498	arg1	profiles					500:507	intact glycoproteomic profiles	478:507	intact glycoproteomic profiles	478:507	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	5	78	theme	clinical	880:887	arg1	outcome					889:895	a poor clinical outcome	873:895	a poor clinical outcome of HGSC	873:903	Further survival analysis reveals that intact glycopeptide signatures of mesenchymal subtype are associated with a poor clinical outcome of HGSC.					
33262351	4	79	theme	strong	597:602	arg1	relationship					604:615	a strong relationship	595:615	a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype	595:757	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	0	80	theme	tumor	37:41	arg1	prediction					74:83	tumor subtyping and clinical outcome prediction	37:83	tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer	37:119	Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer.					
33262351	5	81	gly	glycopeptide	806:817	arg2	glycopeptide					806:817	intact glycopeptide signatures	799:828	intact glycopeptide signatures of mesenchymal subtype	799:851	Further survival analysis reveals that intact glycopeptide signatures of mesenchymal subtype are associated with a poor clinical outcome of HGSC.					
33262351	3	82	theme	major	522:526	arg1	clusters					534:541	3 major tumor clusters	520:541	3 major tumor clusters	520:541	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	9	83	from	production	1503:1512	arg1	subtypes					1527:1534	different subtypes	1517:1534	different subtypes of HGSC	1517:1542	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	3	84	theme	Cluster	458:464	arg1	analysis					466:473	Cluster analysis	458:473	Cluster analysis of intact glycoproteomic profiles	458:507	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	4	85	with	association	706:716	arg1	subtype					751:757	mesenchymal subtype	739:757	mesenchymal subtype	739:757	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	2	86	theme	HGSC	444:447	arg1	tissues					449:455	119 TCGA HGSC tissues	435:455	119 TCGA HGSC tissues	435:455	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	0	87	theme	outcome	66:72	arg1	prediction					74:83	tumor subtyping and clinical outcome prediction	37:83	tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer	37:119	Glycoproteomics-based signatures for tumor subtyping and clinical outcome prediction of high-grade serous ovarian cancer.					
33262351	7	88	theme	glycosylation	1327:1339	arg1	enzymes					1341:1347	glycosylation enzymes	1327:1347	glycosylation enzymes	1327:1347	The results show that glycoprotein levels are mainly controlled by the expression of their individual proteins, and, furthermore, that the glycoprotein-modifying glycans correspond to the protein levels of glycosylation enzymes.					
33262351	4	89	theme	subtypes	665:672	arg1	association					706:716	the association	702:716	the association of fucosylation with mesenchymal subtype	702:757	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	4	89	theme	subtypes	665:672	arg1	example					679:685	one example	675:685	one example	675:685	It also shows a strong relationship between N-glycan structures and tumor molecular subtypes, one example of which being the association of fucosylation with mesenchymal subtype.					
33262351	6	90	gly	glycoprotein	1071:1082	arg1	glycoprotein					1071:1082	glycoprotein biosynthesis pathways	1071:1104	glycoprotein biosynthesis pathways in each tumor	1071:1118	In addition, we study the expression of mRNAs, proteins, glycosites, and intact glycopeptides, as well as the expression levels of glycosylation enzymes involved in glycoprotein biosynthesis pathways in each tumor.					
33262351	2	91	theme	spectrometry-based	381:398	arg1	characterization					415:430	mass spectrometry-based glycoproteomic characterization	376:430	mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues	376:455	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	9	92	theme	process	1477:1483	arg1	understanding					1442:1454	Deeper understanding	1435:1454	Deeper understanding of the glycosylation process	1435:1483	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	9	92	theme	process	1477:1483	arg1	production					1503:1512	glycosylation production	1489:1512	glycosylation production in different subtypes of HGSC	1489:1542	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
33262351	2	93	theme	119	435:437	arg1	tissues					449:455	119 TCGA HGSC tissues	435:455	119 TCGA HGSC tissues	435:455	To investigate the roles of protein glycosylation in the heterogeneity of high-grade serous ovarian carcinoma (HGSC), we perform mass spectrometry-based glycoproteomic characterization of 119 TCGA HGSC tissues.					
33262351	3	94	theme	glycopeptides	566:578	arg1	clusters					534:541	3 major tumor clusters	520:541	3 major tumor clusters	520:541	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	3	94	theme	glycopeptides	566:578	arg1	groups					549:554	5 groups	547:554	5 groups of intact glycopeptides	547:578	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	3	94	theme	glycopeptides	566:578	arg1	glycopeptides					566:578	intact glycopeptides	559:578	intact glycopeptides	559:578	Cluster analysis of intact glycoproteomic profiles delineates 3 major tumor clusters and 5 groups of intact glycopeptides.					
33262351	9	95	theme	glycosylation	1489:1501	arg1	production					1503:1512	glycosylation production	1489:1512	glycosylation production in different subtypes of HGSC	1489:1542	Deeper understanding of the glycosylation process and glycosylation production in different subtypes of HGSC may provide important clues for precision medicine and tumor-targeted therapy.					
34283362	4	0	theme	semi-automated	860:873	arg1	β-elimination					902:914	semi-automated, high throughput reductive β-elimination	860:914	semi-automated, high throughput reductive β-elimination	860:914	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	6	1	theme	spectrum	1510:1517	arg1	region					1496:1501	the matrix region	1485:1501	the matrix region of the spectrum	1485:1517	The use of ultrahigh resolution MALDI-FTICR MS enabled glycan identification and quantitation in the matrix region of the spectrum.					
34283362	1	2	from	alterations	342:352	arg1	O-glycosylation					357:371	O-glycosylation	357:371	O-glycosylation	357:371	It is known that alterations in O-glycosylation are involved in the development and progression of cancer.					
34283362	4	3	theme	resolution	945:954	arg1	data					983:986	ultrahigh resolution MALDI-FT-ICR MS. Automated data	935:986	ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores	935:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	4	theme	selection	1138:1146	arg1	scores					1230:1235	isotopic pattern quality scores	1205:1235	isotopic pattern quality scores	1205:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	4	theme	selection	1138:1146	arg1	criteria					1148:1155	selection criteria	1138:1155	selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores	1138:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	4	theme	selection	1138:1146	arg1	ratio					1183:1187	signal-to-noise ratio	1167:1187	signal-to-noise ratio	1167:1187	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	4	theme	selection	1138:1146	arg1	error					1195:1199	mass error	1190:1199	mass error	1190:1199	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	3	5	theme	large	610:614	arg1	numbers					616:622	large numbers	610:622	large numbers of samples	610:633	However, the O-glycosylation analysis of large numbers of samples, as required in systems biology and biomarker discovery studies, is often challenging.					
34283362	0	6	theme	cell	281:284	arg1	biology					286:292	cell biology	281:292	cell biology	281:292	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	4	7	theme	reductive	892:900	arg1	β-elimination					902:914	semi-automated, high throughput reductive β-elimination	860:914	semi-automated, high throughput reductive β-elimination	860:914	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	8	theme	pattern	1214:1220	arg1	scores					1230:1235	isotopic pattern quality scores	1205:1235	isotopic pattern quality scores	1205:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	0	9	theme	processes	294:302	arg1	multitude					254:262	a multitude	252:262	a multitude of molecular and cell biology processes occurring in cancer	252:322	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	5	10	theme	large	1316:1320	arg1	oligosaccharides					1322:1337	large oligosaccharides	1316:1337	large oligosaccharides exhibiting complex glycan motifs	1316:1370	A total of 126 O-glycan compositions, ranging from a single monosaccharide to large oligosaccharides exhibiting complex glycan motifs, were detected.					
34283362	0	11	theme	protein	148:154	arg1	O-glycosylation					156:170	protein O-glycosylation	148:170	protein O-glycosylation	148:170	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	7	12	theme	sample	1637:1642	arg1	cohorts					1644:1650	patient sample cohorts	1629:1650	patient sample cohorts	1629:1650	This approach has the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts.					
34283362	4	13	theme	mass	1190:1193	arg1	error					1195:1199	mass error	1190:1199	mass error	1190:1199	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	0	14	theme	in	76:77	arg1	lines					109:113	in vitro established cancer cell lines	76:113	in vitro established cancer cell lines	76:113	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	2	15	dep	makes	457:461	arg1	established					472:482	established	472:482	makes in vitro established cell lines suitable and useful models for studying biological mechanisms in disease	457:566	Their easy accessibility makes in vitro established cell lines suitable and useful models for studying biological mechanisms in disease.					
34283362	4	16	theme	MS.	969:971	arg1	data					983:986	ultrahigh resolution MALDI-FT-ICR MS. Automated data	935:986	ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores	935:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	0	17	theme	established	85:95	arg1	lines					109:113	in vitro established cancer cell lines	76:113	in vitro established cancer cell lines	76:113	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	2	18	theme	models	515:520	arg1	lines					489:493	cell lines	484:493	cell lines suitable and useful models for studying biological mechanisms in disease	484:566	Their easy accessibility makes in vitro established cell lines suitable and useful models for studying biological mechanisms in disease.					
34283362	4	19	theme	colorectal	771:780	arg1	lines					794:798	three human colorectal cancer cell lines	759:798	three human colorectal cancer cell lines	759:798	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	0	20	theme	cell	104:107	arg1	lines					109:113	in vitro established cancer cell lines	76:113	in vitro established cancer cell lines	76:113	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	4	21	from	lines	837:841	arg1	O-glycans					744:752	O-glycans	744:752	O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines	744:841	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	0	22	theme	molecular	267:275	arg1	processes					294:302	molecular and cell biology processes	267:302	molecular and cell biology processes occurring in cancer	267:322	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	0	23	dep	in	76:77	arg1	vitro					79:83	vitro	79:83	vitro	79:83	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	5	24	theme	glycan	1358:1363	arg1	motifs					1365:1370	complex glycan motifs	1350:1370	complex glycan motifs	1350:1370	A total of 126 O-glycan compositions, ranging from a single monosaccharide to large oligosaccharides exhibiting complex glycan motifs, were detected.					
34283362	3	25	theme	systems	651:657	arg1	biology					659:665	systems biology	651:665	systems biology	651:665	However, the O-glycosylation analysis of large numbers of samples, as required in systems biology and biomarker discovery studies, is often challenging.					
34283362	4	26	theme	human	808:812	arg1	lines					837:841	two human pancreatic cancer cell lines	804:841	two human pancreatic cancer cell lines	804:841	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	0	27	theme	throughput	23:32	arg1	approach					34:41	A semi-automated, high throughput approach	0:41	approach	34:41	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	0	27	theme	throughput	23:32	arg1	important					175:183	important	175:183	important	175:183	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	3	28	dep	challenging	709:719	arg1	required					639:646	required	639:646	required in systems biology and biomarker discovery studies	639:697	However, the O-glycosylation analysis of large numbers of samples, as required in systems biology and biomarker discovery studies, is often challenging.					
34283362	7	29	theme	O-glycosylation	1567:1581	arg1	analysis					1583:1590	O-glycosylation analysis	1567:1590	O-glycosylation analysis of large numbers of samples	1567:1618	This approach has the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts.					
34283362	4	30	theme	cancer	825:830	arg1	lines					837:841	two human pancreatic cancer cell lines	804:841	two human pancreatic cancer cell lines	804:841	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	0	31	from	research	199:206	arg1	approach					34:41	A semi-automated, high throughput approach	0:41	approach	34:41	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	0	31	from	research	199:206	arg1	important					175:183	important	175:183	important	175:183	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	4	32	theme	cell	789:792	arg1	lines					794:798	three human colorectal cancer cell lines	759:798	three human colorectal cancer cell lines	759:798	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	5	33	theme	O-glycan	1253:1260	arg1	compositions					1262:1273	126 O-glycan compositions	1249:1273	126 O-glycan compositions	1249:1273	A total of 126 O-glycan compositions, ranging from a single monosaccharide to large oligosaccharides exhibiting complex glycan motifs, were detected.					
34283362	1	34	dep	development	393:403	arg1	the					389:391	the	389:391	the	389:391	It is known that alterations in O-glycosylation are involved in the development and progression of cancer.					
34283362	0	35	theme	high	18:21	arg1	approach					34:41	A semi-automated, high throughput approach	0:41	approach	34:41	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	0	35	theme	high	18:21	arg1	important					175:183	important	175:183	important	175:183	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	0	36	theme	O-glycosylation	47:61	arg1	profiling					63:71	O-glycosylation profiling	47:71	O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation	47:170	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	3	37	dep	biology	659:665	arg1	studies					691:697	studies	691:697	studies	691:697	However, the O-glycosylation analysis of large numbers of samples, as required in systems biology and biomarker discovery studies, is often challenging.					
34283362	4	38	theme	relative	1096:1103	arg1	quantitation					1105:1116	relative quantitation	1096:1116	relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores	1096:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	0	39	theme	biological	188:197	arg1	research					199:206	biological research	188:206	biological research	188:206	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	4	40	theme	signal-to-noise	1167:1181	arg1	ratio					1183:1187	signal-to-noise ratio	1167:1187	signal-to-noise ratio	1167:1187	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	41	theme	cell	832:835	arg1	lines					837:841	two human pancreatic cancer cell lines	804:841	two human pancreatic cancer cell lines	804:841	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	6	42	theme	MALDI-FTICR	1420:1430	arg1	MS					1432:1433	ultrahigh resolution MALDI-FTICR MS	1399:1433	ultrahigh resolution MALDI-FTICR MS	1399:1433	The use of ultrahigh resolution MALDI-FTICR MS enabled glycan identification and quantitation in the matrix region of the spectrum.					
34283362	4	43	dep	data	983:986	arg1	performed					1019:1027	performed	1019:1027	was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores	1015:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	44	theme	criteria	1148:1155	arg1	scores					1230:1235	isotopic pattern quality scores	1205:1235	isotopic pattern quality scores	1205:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	44	theme	criteria	1148:1155	arg1	range					1129:1133	a range	1127:1133	a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores	1127:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	44	theme	criteria	1148:1155	arg1	ratio					1183:1187	signal-to-noise ratio	1167:1187	signal-to-noise ratio	1167:1187	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	44	theme	criteria	1148:1155	arg1	error					1195:1199	mass error	1190:1199	mass error	1190:1199	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	2	45	theme	biological	535:544	arg1	mechanisms					546:555	biological mechanisms	535:555	biological mechanisms in disease	535:566	Their easy accessibility makes in vitro established cell lines suitable and useful models for studying biological mechanisms in disease.					
34283362	4	46	theme	quality	1222:1228	arg1	scores					1230:1235	isotopic pattern quality scores	1205:1235	isotopic pattern quality scores	1205:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	3	47	gly	O-glycosylation	582:596	arg1	numbers					616:622	large numbers	610:622	large numbers of samples	610:633	However, the O-glycosylation analysis of large numbers of samples, as required in systems biology and biomarker discovery studies, is often challenging.					
34283362	0	48	theme	cancer	97:102	arg1	lines					109:113	in vitro established cancer cell lines	76:113	in vitro established cancer cell lines	76:113	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	3	49	theme	O-glycosylation	582:596	arg1	analysis					598:605	the O-glycosylation analysis	578:605	the O-glycosylation analysis of large numbers of samples	578:633	However, the O-glycosylation analysis of large numbers of samples, as required in systems biology and biomarker discovery studies, is often challenging.					
34283362	4	50	theme	throughput	881:890	arg1	β-elimination					902:914	semi-automated, high throughput reductive β-elimination	860:914	semi-automated, high throughput reductive β-elimination	860:914	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	5	51	theme	compositions	1262:1273	arg1	total					1240:1244	A total	1238:1244	A total	1238:1244	A total of 126 O-glycan compositions, ranging from a single monosaccharide to large oligosaccharides exhibiting complex glycan motifs, were detected.					
34283362	0	52	theme	O-glycosylation	156:170	arg1	study					139:143	The study	135:143	The study of protein O-glycosylation	135:170	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	3	53	theme	samples	627:633	arg1	numbers					616:622	large numbers	610:622	large numbers of samples	610:633	However, the O-glycosylation analysis of large numbers of samples, as required in systems biology and biomarker discovery studies, is often challenging.					
34283362	0	54	theme	biology	286:292	arg1	processes					294:302	molecular and cell biology processes	267:302	molecular and cell biology processes occurring in cancer	267:322	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	7	55	theme	patient	1629:1635	arg1	cohorts					1644:1650	patient sample cohorts	1629:1650	patient sample cohorts	1629:1650	This approach has the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts.					
34283362	6	56	theme	glycan	1443:1448	arg1	identification					1450:1463	glycan identification	1443:1463	glycan identification	1443:1463	The use of ultrahigh resolution MALDI-FTICR MS enabled glycan identification and quantitation in the matrix region of the spectrum.					
34283362	1	57	theme	cancer	424:429	arg1	progression					409:419	progression	409:419	progression	409:419	It is known that alterations in O-glycosylation are involved in the development and progression of cancer.					
34283362	1	57	theme	cancer	424:429	arg1	development					393:403	development	393:403	development	393:403	It is known that alterations in O-glycosylation are involved in the development and progression of cancer.					
34283362	3	58	theme	numbers	616:622	arg1	analysis					598:605	the O-glycosylation analysis	578:605	the O-glycosylation analysis of large numbers of samples	578:633	However, the O-glycosylation analysis of large numbers of samples, as required in systems biology and biomarker discovery studies, is often challenging.					
34283362	4	59	from	lines	794:798	arg1	O-glycans					744:752	O-glycans	744:752	O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines	744:841	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	7	60	contain	has	1534:1536	arg2	potential					1542:1550	the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts	1538:1650	the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts	1538:1650	This approach has the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts.					
34283362	7	60	contain	has	1534:1536	arg1	approach					1525:1532	This approach	1520:1532	This approach	1520:1532	This approach has the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts.					
34283362	2	61	theme	suitable	495:502	arg1	lines					489:493	cell lines	484:493	cell lines suitable and useful models for studying biological mechanisms in disease	484:566	Their easy accessibility makes in vitro established cell lines suitable and useful models for studying biological mechanisms in disease.					
34283362	4	62	theme	isotopic	1205:1212	arg1	scores					1230:1235	isotopic pattern quality scores	1205:1235	isotopic pattern quality scores	1205:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	6	63	theme	ultrahigh	1399:1407	arg1	MS					1432:1433	ultrahigh resolution MALDI-FTICR MS	1399:1433	ultrahigh resolution MALDI-FTICR MS	1399:1433	The use of ultrahigh resolution MALDI-FTICR MS enabled glycan identification and quantitation in the matrix region of the spectrum.					
34283362	6	64	theme	MS	1432:1433	arg1	use					1392:1394	The use	1388:1394	The use of ultrahigh resolution MALDI-FTICR MS	1388:1433	The use of ultrahigh resolution MALDI-FTICR MS enabled glycan identification and quantitation in the matrix region of the spectrum.					
34283362	7	65	theme	samples	1612:1618	arg1	numbers					1601:1607	large numbers	1595:1607	large numbers of samples	1595:1618	This approach has the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts.					
34283362	2	66	theme	cell	484:487	arg1	lines					489:493	cell lines	484:493	cell lines suitable and useful models for studying biological mechanisms in disease	484:566	Their easy accessibility makes in vitro established cell lines suitable and useful models for studying biological mechanisms in disease.					
34283362	5	67	theme	single	1291:1296	arg1	monosaccharide					1298:1311	a single monosaccharide	1289:1311	a single monosaccharide	1289:1311	A total of 126 O-glycan compositions, ranging from a single monosaccharide to large oligosaccharides exhibiting complex glycan motifs, were detected.					
34283362	6	68	theme	resolution	1409:1418	arg1	MS					1432:1433	ultrahigh resolution MALDI-FTICR MS	1399:1433	ultrahigh resolution MALDI-FTICR MS	1399:1433	The use of ultrahigh resolution MALDI-FTICR MS enabled glycan identification and quantitation in the matrix region of the spectrum.					
34283362	7	69	gly	O-glycosylation	1567:1581	arg1	numbers					1601:1607	large numbers	1595:1607	large numbers of samples	1595:1618	This approach has the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts.					
34283362	7	70	theme	numbers	1601:1607	arg1	analysis					1583:1590	O-glycosylation analysis	1567:1590	O-glycosylation analysis of large numbers of samples	1567:1618	This approach has the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts.					
34283362	4	71	theme	MALDI-FT-ICR	956:967	arg1	data					983:986	ultrahigh resolution MALDI-FT-ICR MS. Automated data	935:986	ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores	935:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	0	72	theme	lines	109:113	arg1	profiling					63:71	O-glycosylation profiling	47:71	O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation	47:170	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	2	73	theme	useful	508:513	arg1	models					515:520	useful models	508:520	useful models for studying biological mechanisms in disease	508:566	Their easy accessibility makes in vitro established cell lines suitable and useful models for studying biological mechanisms in disease.					
34283362	4	74	theme	human	765:769	arg1	lines					794:798	three human colorectal cancer cell lines	759:798	three human colorectal cancer cell lines	759:798	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	0	75	theme	semi-automated	2:15	arg1	approach					34:41	A semi-automated, high throughput approach	0:41	approach	34:41	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	0	75	theme	semi-automated	2:15	arg1	important					175:183	important	175:183	important	175:183	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	2	76	theme	easy	438:441	arg1	accessibility					443:455	Their easy accessibility	432:455	Their easy accessibility	432:455	Their easy accessibility makes in vitro established cell lines suitable and useful models for studying biological mechanisms in disease.					
34283362	4	77	theme	present	729:735	arg1	study					737:741	the present study	725:741	the present study	725:741	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	5	78	theme	complex	1350:1356	arg1	motifs					1365:1370	complex glycan motifs	1350:1370	complex glycan motifs	1350:1370	A total of 126 O-glycan compositions, ranging from a single monosaccharide to large oligosaccharides exhibiting complex glycan motifs, were detected.					
34283362	6	79	theme	matrix	1489:1494	arg1	region					1496:1501	the matrix region	1485:1501	the matrix region of the spectrum	1485:1517	The use of ultrahigh resolution MALDI-FTICR MS enabled glycan identification and quantitation in the matrix region of the spectrum.					
34283362	0	80	from	important	175:183	arg1	research					199:206	biological research	188:206	biological research	188:206	A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS. The study of protein O-glycosylation is important in biological research as O-glycans have been reported to regulate a multitude of molecular and cell biology processes occurring in cancer.					
34283362	7	81	theme	large	1595:1599	arg1	numbers					1601:1607	large numbers	1595:1607	large numbers of samples	1595:1618	This approach has the potential to be used for O-glycosylation analysis of large numbers of samples, such as patient sample cohorts.					
34283362	4	82	theme	Automated	973:981	arg1	data					983:986	ultrahigh resolution MALDI-FT-ICR MS. Automated data	935:986	ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores	935:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	83	dep	semi-automated	860:873	arg1	high					876:879	high	876:879	high	876:879	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	4	84	theme	ultrahigh	935:943	arg1	data					983:986	ultrahigh resolution MALDI-FT-ICR MS. Automated data	935:986	ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores	935:1235	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	3	85	theme	biomarker	671:679	arg1	discovery					681:689	biomarker discovery	671:689	biomarker discovery	671:689	However, the O-glycosylation analysis of large numbers of samples, as required in systems biology and biomarker discovery studies, is often challenging.					
34283362	4	86	theme	pancreatic	814:823	arg1	lines					837:841	two human pancreatic cancer cell lines	804:841	two human pancreatic cancer cell lines	804:841	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	2	87	from	mechanisms	546:555	arg1	disease					560:566	disease	560:566	disease	560:566	Their easy accessibility makes in vitro established cell lines suitable and useful models for studying biological mechanisms in disease.					
34283362	4	88	theme	cancer	782:787	arg1	lines					794:798	three human colorectal cancer cell lines	759:798	three human colorectal cancer cell lines	759:798	In the present study, O-glycans from three human colorectal cancer cell lines and two human pancreatic cancer cell lines were released by semi-automated, high throughput reductive β-elimination and analysed using ultrahigh resolution MALDI-FT-ICR MS. Automated data integration and processing was performed using MassyTools, where the analyte was automatically included for relative quantitation based on a range of selection criteria including signal-to-noise ratio, mass error and isotopic pattern quality scores.					
34283362	3	89	dep	required	639:646	arg1	as					636:637	as	636:637	as	636:637	However, the O-glycosylation analysis of large numbers of samples, as required in systems biology and biomarker discovery studies, is often challenging.					
32280962	0	0	theme	novel	89:93	arg1	ligand					106:111	a novel Galectin-1 ligand	87:111	a novel Galectin-1 ligand	87:111	Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.					
32280962	3	1	with	epitopes	643:650	arg1	sialyation					662:671	α2,3 sialyation	657:671	α2,3 sialyation	657:671	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	1	2	theme	third	339:343	arg1	trimester					345:353	the third trimester	335:353	the third trimester	335:353	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	1	3	from	serum	326:330	arg1	trimester					345:353	the third trimester	335:353	the third trimester	335:353	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	1	4	from	proteins	305:312	arg1	serum					326:330	maternal serum	317:330	maternal serum in the third trimester	317:353	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	6	5	theme	PSG1	1126:1129	arg1	domains					1103:1109	only the N and A2 domains	1085:1109	only the N and A2 domains of recombinant PSG1	1085:1129	In addition, we determined that out of the three N-glycosylation-carrying domains, only the N and A2 domains of recombinant PSG1 interact with Gal-1.					
32280962	6	5	theme	PSG1	1126:1129	arg1	PSG1					1126:1129	recombinant PSG1	1114:1129	recombinant PSG1	1114:1129	In addition, we determined that out of the three N-glycosylation-carrying domains, only the N and A2 domains of recombinant PSG1 interact with Gal-1.					
32280962	0	6	theme	Galectin-1	95:104	arg1	ligand					106:111	a novel Galectin-1 ligand	87:111	a novel Galectin-1 ligand	87:111	Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.					
32280962	4	7	theme	successful	859:868	arg1	pregnancy					870:878	a successful pregnancy	857:878	a successful pregnancy	857:878	Using different techniques, we explored whether PSG1 can bind to galectin-1 (Gal-1) as these two proteins were previously shown to participate in processes required for a successful pregnancy.					
32280962	3	8	theme	pregnant	556:563	arg1	women					565:569	pregnant women	556:569	pregnant women	556:569	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	6	9	theme	A2	1100:1101	arg1	domains					1103:1109	only the N and A2 domains	1085:1109	only the N and A2 domains of recombinant PSG1	1085:1129	In addition, we determined that out of the three N-glycosylation-carrying domains, only the N and A2 domains of recombinant PSG1 interact with Gal-1.					
32280962	6	9	theme	A2	1100:1101	arg1	PSG1					1126:1129	recombinant PSG1	1114:1129	recombinant PSG1	1114:1129	In addition, we determined that out of the three N-glycosylation-carrying domains, only the N and A2 domains of recombinant PSG1 interact with Gal-1.					
32280962	6	10	theme	recombinant	1114:1124	arg1	PSG1					1126:1129	recombinant PSG1	1114:1129	recombinant PSG1	1114:1129	In addition, we determined that out of the three N-glycosylation-carrying domains, only the N and A2 domains of recombinant PSG1 interact with Gal-1.					
32280962	2	11	gly	glycosylation	390:402	arg2	seven					365:369	seven	365:369	seven	365:369	PSG1 has seven potential N-linked glycosylation sites across its four domains.					
32280962	2	11	gly	glycosylation	390:402	arg2	sites					404:408	seven potential N-linked glycosylation sites	365:408	seven potential N-linked glycosylation sites	365:408	PSG1 has seven potential N-linked glycosylation sites across its four domains.					
32280962	6	12	theme	N-glycosylation-carrying	1051:1074	arg1	domains					1076:1082	the three N-glycosylation-carrying domains	1041:1082	the three N-glycosylation-carrying domains	1041:1082	In addition, we determined that out of the three N-glycosylation-carrying domains, only the N and A2 domains of recombinant PSG1 interact with Gal-1.					
32280962	2	13	contain	has	361:363	arg1	PSG1					356:359	PSG1	356:359	PSG1	356:359	PSG1 has seven potential N-linked glycosylation sites across its four domains.					
32280962	2	13	contain	has	361:363	arg2	sites					404:408	seven potential N-linked glycosylation sites	365:408	seven potential N-linked glycosylation sites	365:408	PSG1 has seven potential N-linked glycosylation sites across its four domains.					
32280962	5	14	theme	interaction	978:988	arg1	affinity					962:969	an affinity	959:969	an affinity of the interaction of 0.13 μM	959:999	We confirmed that PSG1 binds to Gal-1 in a carbohydrate-dependent manner with an affinity of the interaction of 0.13 μM.					
32280962	1	15	theme	trophoblast	177:187	arg1	cells					189:193	trophoblast cells	177:193	trophoblast cells of the human placenta	177:215	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	5	16	theme	carbohydrate-dependent	924:945	arg1	manner					947:952	a carbohydrate-dependent manner	922:952	a carbohydrate-dependent manner with an affinity of the interaction of 0.13 μM	922:999	We confirmed that PSG1 binds to Gal-1 in a carbohydrate-dependent manner with an affinity of the interaction of 0.13 μM.					
32280962	2	17	theme	N-linked	381:388	arg1	sites					404:408	seven potential N-linked glycosylation sites	365:408	seven potential N-linked glycosylation sites	365:408	PSG1 has seven potential N-linked glycosylation sites across its four domains.					
32280962	0	18	theme	Glycan	0:5	arg1	characterization					7:22	Glycan characterization	0:22	Glycan characterization of pregnancy-specific glycoprotein 1	0:59	Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.					
32280962	2	19	theme	potential	371:379	arg1	sites					404:408	seven potential N-linked glycosylation sites	365:408	seven potential N-linked glycosylation sites	365:408	PSG1 has seven potential N-linked glycosylation sites across its four domains.					
32280962	0	20	theme	pregnancy-specific	27:44	arg1	glycoprotein					46:57	pregnancy-specific glycoprotein 1	27:59	pregnancy-specific glycoprotein 1	27:59	Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.					
32280962	3	21	theme	women	565:569	arg1	serum					547:551	serum	547:551	serum of pregnant women	547:569	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	3	22	theme	glycoproteomic	463:476	arg1	studies					478:484	glycomic and glycoproteomic studies	450:484	glycomic and glycoproteomic studies	450:484	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	5	23	with	manner	947:952	arg1	affinity					962:969	an affinity	959:969	an affinity of the interaction of 0.13 μM	959:999	We confirmed that PSG1 binds to Gal-1 in a carbohydrate-dependent manner with an affinity of the interaction of 0.13 μM.					
32280962	2	24	link	N-linked	381:388	arg1	sites					404:408	seven potential N-linked glycosylation sites	365:408	seven potential N-linked glycosylation sites	365:408	PSG1 has seven potential N-linked glycosylation sites across its four domains.					
32280962	1	25	theme	Pregnancy-specific	114:131	arg1	glycoprotein					140:151	Pregnancy-specific beta 1 glycoprotein	114:151	Pregnancy-specific beta 1 glycoprotein (PSG1)	114:158	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	1	25	theme	Pregnancy-specific	114:131	arg1	PSG1					154:157	PSG1	154:157	PSG1	154:157	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	1	26	theme	human	202:206	arg1	placenta					208:215	the human placenta	198:215	the human placenta	198:215	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	7	27	gly	glycoprotein	1344:1355	arg1	glycoprotein					1344:1355	its glycoprotein ligands	1340:1363	its glycoprotein ligands	1340:1363	Lastly, we observed that the interaction between PSG1 and Gal-1 protects this lectin from oxidative inactivation and that PSG1 competes the ability of Gal-1 to bind to some but not all of its glycoprotein ligands.					
32280962	1	28	theme	abundant	296:303	arg1	proteins					305:312	the most abundant proteins	287:312	the most abundant proteins in maternal serum in the third trimester	287:353	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	3	29	theme	glycomic	450:457	arg1	studies					478:484	glycomic and glycoproteomic studies	450:484	glycomic and glycoproteomic studies	450:484	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	3	30	theme	complex	602:608	arg1	N-glycans					610:618	complex N-glycans	602:618	complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites	602:685	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	7	31	theme	Gal-1	1303:1307	arg1	ability					1292:1298	the ability	1288:1298	the ability of Gal-1 to bind to some but not all of its glycoprotein ligands	1288:1363	Lastly, we observed that the interaction between PSG1 and Gal-1 protects this lectin from oxidative inactivation and that PSG1 competes the ability of Gal-1 to bind to some but not all of its glycoprotein ligands.					
32280962	1	32	theme	placenta	208:215	arg1	cells					189:193	trophoblast cells	177:193	trophoblast cells of the human placenta	177:215	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	0	33	theme	glycoprotein	46:57	arg1	identification					69:82	its identification	65:82	its identification as a novel Galectin-1 ligand	65:111	Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.					
32280962	0	33	theme	glycoprotein	46:57	arg1	characterization					7:22	Glycan characterization	0:22	Glycan characterization of pregnancy-specific glycoprotein 1	0:59	Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.					
32280962	1	34	theme	proteins	305:312	arg1	proteins					305:312	the most abundant proteins	287:312	the most abundant proteins in maternal serum in the third trimester	287:353	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	1	34	theme	proteins	305:312	arg1	one					280:282	one	280:282	one	280:282	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	1	35	gly	glycoprotein	140:151	arg1	glycoprotein					140:151	Pregnancy-specific beta 1 glycoprotein	114:151	Pregnancy-specific beta 1 glycoprotein (PSG1)	114:158	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	1	35	gly	glycoprotein	140:151	arg1	PSG1					154:157	PSG1	154:157	PSG1	154:157	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	3	36	theme	N-glycans	610:618	arg1	presence					590:597	the presence	586:597	the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites	586:685	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	3	37	theme	α2,3	657:660	arg1	sialyation					662:671	α2,3 sialyation	657:671	α2,3 sialyation	657:671	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	1	38	theme	beta	133:136	arg1	glycoprotein					140:151	Pregnancy-specific beta 1 glycoprotein	114:151	Pregnancy-specific beta 1 glycoprotein (PSG1)	114:158	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	1	38	theme	beta	133:136	arg1	PSG1					154:157	PSG1	154:157	PSG1	154:157	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	1	39	theme	maternal	317:324	arg1	serum					326:330	maternal serum	317:330	maternal serum in the third trimester	317:353	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	3	40	theme	glycan	506:511	arg1	composition					513:523	the glycan composition	502:523	the glycan composition of PSG1 purified from serum of pregnant women	502:569	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	3	41	theme	LacNAc	636:641	arg1	epitopes					643:650	poly LacNAc epitopes	631:650	poly LacNAc epitopes with α2,3 sialyation	631:671	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	0	42	gly	glycoprotein	46:57	arg1	glycoprotein					46:57	pregnancy-specific glycoprotein 1	27:59	pregnancy-specific glycoprotein 1	27:59	Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.					
32280962	7	43	theme	glycoprotein	1344:1355	arg1	ligands					1357:1363	its glycoprotein ligands	1340:1363	its glycoprotein ligands	1340:1363	Lastly, we observed that the interaction between PSG1 and Gal-1 protects this lectin from oxidative inactivation and that PSG1 competes the ability of Gal-1 to bind to some but not all of its glycoprotein ligands.					
32280962	7	44	theme	oxidative	1242:1250	arg1	inactivation					1252:1263	oxidative inactivation	1242:1263	oxidative inactivation	1242:1263	Lastly, we observed that the interaction between PSG1 and Gal-1 protects this lectin from oxidative inactivation and that PSG1 competes the ability of Gal-1 to bind to some but not all of its glycoprotein ligands.					
32280962	1	45	theme	increasing	220:229	arg1	concentrations					231:244	increasing concentrations	220:244	increasing concentrations as pregnancy progresses	220:268	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
32280962	5	46	theme	0.13 μM	993:999	arg1	interaction					978:988	the interaction	974:988	the interaction of 0.13 μM	974:999	We confirmed that PSG1 binds to Gal-1 in a carbohydrate-dependent manner with an affinity of the interaction of 0.13 μM.					
32280962	6	47	theme	N	1094:1094	arg1	domains					1103:1109	only the N and A2 domains	1085:1109	only the N and A2 domains of recombinant PSG1	1085:1129	In addition, we determined that out of the three N-glycosylation-carrying domains, only the N and A2 domains of recombinant PSG1 interact with Gal-1.					
32280962	6	47	theme	N	1094:1094	arg1	PSG1					1126:1129	recombinant PSG1	1114:1129	recombinant PSG1	1114:1129	In addition, we determined that out of the three N-glycosylation-carrying domains, only the N and A2 domains of recombinant PSG1 interact with Gal-1.					
32280962	3	48	contain	containing	620:629	arg2	epitopes					643:650	poly LacNAc epitopes	631:650	poly LacNAc epitopes with α2,3 sialyation	631:671	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	3	48	contain	containing	620:629	arg1	N-glycans					610:618	complex N-glycans	602:618	complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites	602:685	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	3	49	contain	carried	438:444	arg2	studies					478:484	glycomic and glycoproteomic studies	450:484	glycomic and glycoproteomic studies	450:484	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	3	49	contain	carried	438:444	arg1	We					435:436	We	435:436	We	435:436	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	3	50	theme	PSG1	528:531	arg1	composition					513:523	the glycan composition	502:523	the glycan composition of PSG1 purified from serum of pregnant women	502:569	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	2	51	theme	glycosylation	390:402	arg1	sites					404:408	seven potential N-linked glycosylation sites	365:408	seven potential N-linked glycosylation sites	365:408	PSG1 has seven potential N-linked glycosylation sites across its four domains.					
32280962	3	52	theme	poly	631:634	arg1	epitopes					643:650	poly LacNAc epitopes	631:650	poly LacNAc epitopes with α2,3 sialyation	631:671	We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites.					
32280962	4	53	theme	different	694:702	arg1	techniques					704:713	different techniques	694:713	different techniques	694:713	Using different techniques, we explored whether PSG1 can bind to galectin-1 (Gal-1) as these two proteins were previously shown to participate in processes required for a successful pregnancy.					
32280962	1	54	theme	pregnancy	249:257	arg1	progresses					259:268	pregnancy progresses	249:268	pregnancy progresses	249:268	Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester.					
33876698	7	0	theme	active	1188:1193	arg1	site					1195:1198	the active site	1184:1198	the active site	1184:1198	But the molecular modeling suggests that the most favorable binding sites are located outside the active site.					
33876698	11	1	theme	asparagine	1615:1624	arg1	N197					1635:1638	N197	1635:1638	N197	1635:1638	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	1	theme	asparagine	1615:1624	arg1	N232					1650:1653	N232	1650:1653	N232	1650:1653	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	1	theme	asparagine	1615:1624	arg1	residues					1626:1633	asparagine residues N197, N212 and N232	1615:1653	asparagine residues N197, N212 and N232	1615:1653	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	1	theme	asparagine	1615:1624	arg1	N212					1641:1644	N212	1641:1644	N212	1641:1644	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	2	theme	tetra-antennary	1565:1579	arg1	glycan					1589:1594	a bulkier tetra-antennary complex glycan	1555:1594	a bulkier tetra-antennary complex glycan	1555:1594	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	12	3	theme	Ramaswamy	1797:1805	arg1	Sarma					1810:1814	Ramaswamy H. Sarma	1797:1814	Ramaswamy H. Sarma	1797:1814	These N-glycans do not impede drug binding, thus suggesting that all glycoforms of PPT1 can be targeted with these compounds.Communicated by Ramaswamy H. Sarma.					
33876698	6	4	theme	palmitate	984:992	arg1	site					994:997	the palmitate site	980:997	the palmitate site of the protein	980:1012	The molecules can fit into the palmitate site of the protein, with the dimeric compounds forming more stable complexes than the monomer.					
33876698	6	4	theme	palmitate	984:992	arg1	protein					1006:1012	the protein	1002:1012	the protein	1002:1012	The molecules can fit into the palmitate site of the protein, with the dimeric compounds forming more stable complexes than the monomer.					
33876698	7	5	theme	molecular	1098:1106	arg1	modeling					1108:1115	the molecular modeling	1094:1115	the molecular modeling	1094:1115	But the molecular modeling suggests that the most favorable binding sites are located outside the active site.					
33876698	9	6	theme	stable	1411:1416	arg1	complex					1418:1424	the most stable complex	1402:1424	the most stable complex	1402:1424	According to the calculated empirical energies of interaction (ΔE), the dimer DC661 forms the most stable complex at site Met112 of palmitate-bound PPT1.					
33876698	1	7	theme	lysosomal	154:162	arg1	thioesterase					189:200	The lysosomal enzyme palmitoyl-protein thioesterase 1	150:202	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1)	150:209	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	1	7	theme	lysosomal	154:162	arg1	PPT1					205:208	PPT1	205:208	PPT1	205:208	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	1	8	theme	membrane-bound	259:272	arg1	proteins					274:281	membrane-bound proteins	259:281	membrane-bound proteins	259:281	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	11	9	theme	Paucimannosidic	1512:1526	arg1	M3F					1546:1548	M3F	1546:1548	M3F	1546:1548	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	9	theme	Paucimannosidic	1512:1526	arg1	M2FA					1537:1540	M2FA	1537:1540	M2FA	1537:1540	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	9	theme	Paucimannosidic	1512:1526	arg1	glycans					1528:1534	Paucimannosidic glycans	1512:1534	Paucimannosidic glycans (M2FA and M3F)	1512:1549	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	5	10	theme	structure	942:950	arg1	advantage					903:911	advantage	903:911	advantage of the PPT1 crystallographic structure	903:950	We have modeled the interaction of these three compounds with the enzyme, taking advantage of the PPT1 crystallographic structure.					
33876698	9	11	theme	dimer	1384:1388	arg1	DC661					1390:1394	the dimer DC661	1380:1394	the dimer DC661	1380:1394	According to the calculated empirical energies of interaction (ΔE), the dimer DC661 forms the most stable complex at site Met112 of palmitate-bound PPT1.					
33876698	8	12	dep	residues	1223:1230	arg1	residues					1223:1230	residues	1223:1230	residues Met112 and Gln144	1223:1248	Two sites centered on residues Met112 and Gln144 were identified, offering suitable cavities for drug binding.					
33876698	8	12	dep	residues	1223:1230	arg1	Gln144					1243:1248	Gln144	1243:1248	Gln144	1243:1248	Two sites centered on residues Met112 and Gln144 were identified, offering suitable cavities for drug binding.					
33876698	8	12	dep	residues	1223:1230	arg1	Met112					1232:1237	Met112	1232:1237	Met112	1232:1237	Two sites centered on residues Met112 and Gln144 were identified, offering suitable cavities for drug binding.					
33876698	4	13	theme	particular	696:705	arg1	analogues					795:803	the dimeric analogues Lys05 and DC661	783:819	the dimeric analogues Lys05 and DC661	783:819	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	4	13	theme	particular	696:705	arg1	derivatives					734:744	particular 4-amino-7-chloro-quinoline derivatives	696:744	particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661	696:819	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	4	13	theme	particular	696:705	arg1	hydroxychloroquine					754:771	hydroxychloroquine	754:771	hydroxychloroquine (HCQ)	754:777	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	0	14	theme	glycosylated	104:115	arg1	forms					117:121	its glycosylated forms	100:121	its glycosylated forms	100:121	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	7	15	theme	favorable	1140:1148	arg1	located					1168:1174	located	1168:1174	located	1168:1174	But the molecular modeling suggests that the most favorable binding sites are located outside the active site.					
33876698	7	15	theme	favorable	1140:1148	arg1	sites					1158:1162	the most favorable binding sites	1131:1162	the most favorable binding sites	1131:1162	But the molecular modeling suggests that the most favorable binding sites are located outside the active site.					
33876698	6	16	theme	stable	1055:1060	arg1	complexes					1062:1070	more stable complexes	1050:1070	more stable complexes than the monomer	1050:1087	The molecules can fit into the palmitate site of the protein, with the dimeric compounds forming more stable complexes than the monomer.					
33876698	2	17	theme	cortical	387:394	arg1	neurons					396:402	cortical neurons	387:402	cortical neurons	387:402	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	12	18	theme	H.	1807:1808	arg1	Sarma					1810:1814	Ramaswamy H. Sarma	1797:1814	Ramaswamy H. Sarma	1797:1814	These N-glycans do not impede drug binding, thus suggesting that all glycoforms of PPT1 can be targeted with these compounds.Communicated by Ramaswamy H. Sarma.					
33876698	10	19	gly	N-glycosylated	1466:1479	arg1	forms					1481:1485	N-glycosylated forms	1466:1485	N-glycosylated forms of PPT1	1466:1493	N-glycosylated forms of PPT1 were elaborated.					
33876698	10	19	gly	N-glycosylated	1466:1479	arg1	PPT1					1490:1493	PPT1	1490:1493	PPT1	1490:1493	N-glycosylated forms of PPT1 were elaborated.					
33876698	1	20	theme	palmitoyl-protein	171:187	arg1	thioesterase					189:200	The lysosomal enzyme palmitoyl-protein thioesterase 1	150:202	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1)	150:209	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	1	20	theme	palmitoyl-protein	171:187	arg1	PPT1					205:208	PPT1	205:208	PPT1	205:208	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	2	21	theme	ceroid	444:449	arg1	INCL					467:470	INCL	467:470	INCL	467:470	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	21	theme	ceroid	444:449	arg1	disorder					501:508	a severe neurodegenerative disorder	474:508	a severe neurodegenerative disorder in children	474:520	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	21	theme	ceroid	444:449	arg1	lipofuscinosis					451:464	infantile neural ceroid lipofuscinosis	427:464	infantile neural ceroid lipofuscinosis (INCL)	427:471	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	11	22	dep	residues	1626:1633	arg1	N197					1635:1638	N197	1635:1638	N197	1635:1638	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	22	dep	residues	1626:1633	arg1	N232					1650:1653	N232	1650:1653	N232	1650:1653	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	22	dep	residues	1626:1633	arg1	residues					1626:1633	asparagine residues N197, N212 and N232	1615:1653	asparagine residues N197, N212 and N232	1615:1653	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	22	dep	residues	1626:1633	arg1	N212					1641:1644	N212	1641:1644	N212	1641:1644	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	12	23	theme	drug	1686:1689	arg1	binding					1691:1697	drug binding	1686:1697	drug binding	1686:1697	These N-glycans do not impede drug binding, thus suggesting that all glycoforms of PPT1 can be targeted with these compounds.Communicated by Ramaswamy H. Sarma.					
33876698	2	24	theme	PPT1	326:329	arg1	lack					318:321	A lack	316:321	A lack of PPT1 (due to gene mutations)	316:353	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	24	theme	PPT1	326:329	arg1	responsible					411:421	responsible	411:421	responsible	411:421	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	7	25	theme	binding	1150:1156	arg1	located					1168:1174	located	1168:1174	located	1168:1174	But the molecular modeling suggests that the most favorable binding sites are located outside the active site.					
33876698	7	25	theme	binding	1150:1156	arg1	sites					1158:1162	the most favorable binding sites	1131:1162	the most favorable binding sites	1131:1162	But the molecular modeling suggests that the most favorable binding sites are located outside the active site.					
33876698	2	26	theme	neural	437:442	arg1	INCL					467:470	INCL	467:470	INCL	467:470	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	26	theme	neural	437:442	arg1	disorder					501:508	a severe neurodegenerative disorder	474:508	a severe neurodegenerative disorder in children	474:520	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	26	theme	neural	437:442	arg1	lipofuscinosis					451:464	infantile neural ceroid lipofuscinosis	427:464	infantile neural ceroid lipofuscinosis (INCL)	427:471	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	1	27	link	thioester-linked	219:234	arg1	acid					242:245	thioester-linked fatty acid	219:245	thioester-linked fatty acid groups	219:252	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	4	28	theme	4-amino-7-chloro-quinoline	707:732	arg1	analogues					795:803	the dimeric analogues Lys05 and DC661	783:819	the dimeric analogues Lys05 and DC661	783:819	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	4	28	theme	4-amino-7-chloro-quinoline	707:732	arg1	derivatives					734:744	particular 4-amino-7-chloro-quinoline derivatives	696:744	particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661	696:819	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	4	28	theme	4-amino-7-chloro-quinoline	707:732	arg1	hydroxychloroquine					754:771	hydroxychloroquine	754:771	hydroxychloroquine (HCQ)	754:777	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	2	29	theme	progressive	366:376	arg1	death					378:382	the progressive death	362:382	the progressive death of cortical neurons	362:402	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	30	theme	infantile	427:435	arg1	INCL					467:470	INCL	467:470	INCL	467:470	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	30	theme	infantile	427:435	arg1	disorder					501:508	a severe neurodegenerative disorder	474:508	a severe neurodegenerative disorder in children	474:520	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	30	theme	infantile	427:435	arg1	lipofuscinosis					451:464	infantile neural ceroid lipofuscinosis	427:464	infantile neural ceroid lipofuscinosis (INCL)	427:471	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	8	31	theme	suitable	1276:1283	arg1	cavities					1285:1292	suitable cavities	1276:1292	suitable cavities for drug binding	1276:1309	Two sites centered on residues Met112 and Gln144 were identified, offering suitable cavities for drug binding.					
33876698	5	32	theme	crystallographic	925:940	arg1	structure					942:950	the PPT1 crystallographic structure	916:950	the PPT1 crystallographic structure	916:950	We have modeled the interaction of these three compounds with the enzyme, taking advantage of the PPT1 crystallographic structure.					
33876698	6	33	theme	dimeric	1024:1030	arg1	compounds					1032:1040	the dimeric compounds	1020:1040	the dimeric compounds forming more stable complexes than the monomer	1020:1087	The molecules can fit into the palmitate site of the protein, with the dimeric compounds forming more stable complexes than the monomer.					
33876698	0	34	theme	hydroxychloroquine	11:28	arg1	Binding					0:6	Binding	0:6	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1)	0:94	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	0	34	theme	hydroxychloroquine	11:28	arg1	forms					117:121	its glycosylated forms	100:121	its glycosylated forms	100:121	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	5	35	theme	PPT1	920:923	arg1	structure					942:950	the PPT1 crystallographic structure	916:950	the PPT1 crystallographic structure	916:950	We have modeled the interaction of these three compounds with the enzyme, taking advantage of the PPT1 crystallographic structure.					
33876698	0	36	gly	glycosylated	104:115	arg1	hydroxychloroquine					11:28	hydroxychloroquine	11:28	hydroxychloroquine	11:28	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	0	36	gly	glycosylated	104:115	arg1	forms					117:121	its glycosylated forms	100:121	its glycosylated forms	100:121	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	0	36	gly	glycosylated	104:115	arg1	chloroquine					34:44	chloroquine	34:44	chloroquine	34:44	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	5	37	theme	compounds	869:877	arg1	interaction					842:852	the interaction	838:852	the interaction of these three compounds with the enzyme	838:893	We have modeled the interaction of these three compounds with the enzyme, taking advantage of the PPT1 crystallographic structure.					
33876698	8	38	theme	drug	1298:1301	arg1	binding					1303:1309	drug binding	1298:1309	drug binding	1298:1309	Two sites centered on residues Met112 and Gln144 were identified, offering suitable cavities for drug binding.					
33876698	0	39	theme	computational	126:138	arg1	approach					140:147	a computational approach	124:147	a computational approach	124:147	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	12	40	gly	glycoforms	1725:1734	arg1	PPT1					1739:1742	PPT1	1739:1742	PPT1	1739:1742	These N-glycans do not impede drug binding, thus suggesting that all glycoforms of PPT1 can be targeted with these compounds.Communicated by Ramaswamy H. Sarma.					
33876698	4	41	theme	selective	644:652	arg1	inhibitors					654:663	Potent and selective inhibitors	633:663	Potent and selective inhibitors of PPT1	633:671	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	9	42	theme	site	1429:1432	arg1	Met112					1434:1439	site Met112	1429:1439	site Met112 of palmitate-bound PPT1	1429:1463	According to the calculated empirical energies of interaction (ΔE), the dimer DC661 forms the most stable complex at site Met112 of palmitate-bound PPT1.					
33876698	1	43	theme	enzyme	164:169	arg1	thioesterase					189:200	The lysosomal enzyme palmitoyl-protein thioesterase 1	150:202	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1)	150:209	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	1	43	theme	enzyme	164:169	arg1	PPT1					205:208	PPT1	205:208	PPT1	205:208	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	9	44	theme	calculated	1329:1338	arg1	energies					1350:1357	the calculated empirical energies	1325:1357	the calculated empirical energies of interaction (ΔE)	1325:1377	According to the calculated empirical energies of interaction (ΔE), the dimer DC661 forms the most stable complex at site Met112 of palmitate-bound PPT1.					
33876698	0	45	theme	chloroquine	34:44	arg1	Binding					0:6	Binding	0:6	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1)	0:94	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	0	45	theme	chloroquine	34:44	arg1	forms					117:121	its glycosylated forms	100:121	its glycosylated forms	100:121	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	2	46	theme	neurons	396:402	arg1	death					378:382	the progressive death	362:382	the progressive death of cortical neurons	362:402	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	0	47	theme	palmitoyl-protein	56:72	arg1	thioesterase					74:85	palmitoyl-protein thioesterase 1	56:87	palmitoyl-protein thioesterase 1 (PPT1)	56:94	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	0	47	theme	palmitoyl-protein	56:72	arg1	PPT1					90:93	PPT1	90:93	PPT1	90:93	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	9	48	theme	empirical	1340:1348	arg1	energies					1350:1357	the calculated empirical energies	1325:1357	the calculated empirical energies of interaction (ΔE)	1325:1377	According to the calculated empirical energies of interaction (ΔE), the dimer DC661 forms the most stable complex at site Met112 of palmitate-bound PPT1.					
33876698	10	49	theme	PPT1	1490:1493	arg1	forms					1481:1485	N-glycosylated forms	1466:1485	N-glycosylated forms of PPT1	1466:1493	N-glycosylated forms of PPT1 were elaborated.					
33876698	3	50	theme	tumor	619:623	arg1	growth					625:630	tumor growth	619:630	tumor growth	619:630	Conversely, PPT1 is often over-expressed in cancer, and considered as a valid target to control tumor growth.					
33876698	2	51	theme	gene	339:342	arg1	mutations					344:352	gene mutations	339:352	gene mutations	339:352	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	4	52	dep	analogues	795:803	arg1	analogues					795:803	the dimeric analogues Lys05 and DC661	783:819	the dimeric analogues Lys05 and DC661	783:819	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	4	52	dep	analogues	795:803	arg1	DC661					815:819	DC661	815:819	DC661	815:819	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	4	52	dep	analogues	795:803	arg1	Lys05					805:809	Lys05	805:809	Lys05	805:809	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	9	53	theme	PPT1	1460:1463	arg1	Met112					1434:1439	site Met112	1429:1439	site Met112 of palmitate-bound PPT1	1429:1463	According to the calculated empirical energies of interaction (ΔE), the dimer DC661 forms the most stable complex at site Met112 of palmitate-bound PPT1.					
33876698	9	54	theme	palmitate-bound	1444:1458	arg1	PPT1					1460:1463	palmitate-bound PPT1	1444:1463	palmitate-bound PPT1	1444:1463	According to the calculated empirical energies of interaction (ΔE), the dimer DC661 forms the most stable complex at site Met112 of palmitate-bound PPT1.					
33876698	12	55	theme	PPT1	1739:1742	arg1	glycoforms					1725:1734	all glycoforms	1721:1734	all glycoforms of PPT1	1721:1742	These N-glycans do not impede drug binding, thus suggesting that all glycoforms of PPT1 can be targeted with these compounds.Communicated by Ramaswamy H. Sarma.					
33876698	4	56	theme	dimeric	787:793	arg1	analogues					795:803	the dimeric analogues Lys05 and DC661	783:819	the dimeric analogues Lys05 and DC661	783:819	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	4	56	theme	dimeric	787:793	arg1	DC661					815:819	DC661	815:819	DC661	815:819	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	4	56	theme	dimeric	787:793	arg1	Lys05					805:809	Lys05	805:809	Lys05	805:809	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	5	57	with	interaction	842:852	arg1	enzyme					888:893	the enzyme	884:893	the enzyme	884:893	We have modeled the interaction of these three compounds with the enzyme, taking advantage of the PPT1 crystallographic structure.					
33876698	2	58	from	disorder	501:508	arg1	children					513:520	children	513:520	children	513:520	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	59	theme	due	332:334	arg1	lack					318:321	A lack	316:321	A lack of PPT1 (due to gene mutations)	316:353	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	59	theme	due	332:334	arg1	responsible					411:421	responsible	411:421	responsible	411:421	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	9	60	theme	interaction	1362:1372	arg1	energies					1350:1357	the calculated empirical energies	1325:1357	the calculated empirical energies of interaction (ΔE)	1325:1377	According to the calculated empirical energies of interaction (ΔE), the dimer DC661 forms the most stable complex at site Met112 of palmitate-bound PPT1.					
33876698	1	61	theme	thioester-linked	219:234	arg1	acid					242:245	thioester-linked fatty acid	219:245	thioester-linked fatty acid groups	219:252	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	10	62	theme	N-glycosylated	1466:1479	arg1	forms					1481:1485	N-glycosylated forms	1466:1485	N-glycosylated forms of PPT1	1466:1493	N-glycosylated forms of PPT1 were elaborated.					
33876698	0	63	dep	hydroxychloroquine	11:28	arg1	dimers					46:51	dimers	46:51	dimers	46:51	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	11	64	theme	complex	1581:1587	arg1	glycan					1589:1594	a bulkier tetra-antennary complex glycan	1555:1594	a bulkier tetra-antennary complex glycan	1555:1594	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	0	65	dep	approach	140:147	arg1	Binding					0:6	Binding	0:6	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1)	0:94	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	0	65	dep	approach	140:147	arg1	forms					117:121	its glycosylated forms	100:121	its glycosylated forms	100:121	Binding of hydroxychloroquine and chloroquine dimers to palmitoyl-protein thioesterase 1 (PPT1) and its glycosylated forms: a computational approach.					
33876698	1	66	theme	fatty	236:240	arg1	acid					242:245	thioester-linked fatty acid	219:245	thioester-linked fatty acid groups	219:252	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	2	67	theme	neurodegenerative	483:499	arg1	lipofuscinosis					451:464	infantile neural ceroid lipofuscinosis	427:464	infantile neural ceroid lipofuscinosis (INCL)	427:471	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	67	theme	neurodegenerative	483:499	arg1	disorder					501:508	a severe neurodegenerative disorder	474:508	a severe neurodegenerative disorder in children	474:520	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	4	68	theme	PPT1	668:671	arg1	inhibitors					654:663	Potent and selective inhibitors	633:663	Potent and selective inhibitors of PPT1	633:671	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	4	69	theme	Potent	633:638	arg1	inhibitors					654:663	Potent and selective inhibitors	633:663	Potent and selective inhibitors of PPT1	633:671	Potent and selective inhibitors of PPT1 have been designed, in particular 4-amino-7-chloro-quinoline derivatives such as hydroxychloroquine (HCQ) and the dimeric analogues Lys05 and DC661.					
33876698	1	70	theme	acid	242:245	arg1	groups					247:252	thioester-linked fatty acid groups	219:252	thioester-linked fatty acid groups	219:252	The lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) removes thioester-linked fatty acid groups from membrane-bound proteins to facilitate their proteolysis.					
33876698	2	71	theme	severe	476:481	arg1	lipofuscinosis					451:464	infantile neural ceroid lipofuscinosis	427:464	infantile neural ceroid lipofuscinosis (INCL)	427:471	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	2	71	theme	severe	476:481	arg1	disorder					501:508	a severe neurodegenerative disorder	474:508	a severe neurodegenerative disorder in children	474:520	A lack of PPT1 (due to gene mutations) causes the progressive death of cortical neurons and is responsible for infantile neural ceroid lipofuscinosis (INCL), a severe neurodegenerative disorder in children.					
33876698	3	72	theme	valid	595:599	arg1	PPT1					535:538	PPT1	535:538	PPT1	535:538	Conversely, PPT1 is often over-expressed in cancer, and considered as a valid target to control tumor growth.					
33876698	3	72	theme	valid	595:599	arg1	target					601:606	a valid target	593:606	a valid target to control tumor growth	593:630	Conversely, PPT1 is often over-expressed in cancer, and considered as a valid target to control tumor growth.					
33876698	11	73	theme	bulkier	1557:1563	arg1	glycan					1589:1594	a bulkier tetra-antennary complex glycan	1555:1594	a bulkier tetra-antennary complex glycan	1555:1594	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	6	74	theme	protein	1006:1012	arg1	site					994:997	the palmitate site	980:997	the palmitate site of the protein	980:1012	The molecules can fit into the palmitate site of the protein, with the dimeric compounds forming more stable complexes than the monomer.					
33876698	6	74	theme	protein	1006:1012	arg1	protein					1006:1012	the protein	1002:1012	the protein	1002:1012	The molecules can fit into the palmitate site of the protein, with the dimeric compounds forming more stable complexes than the monomer.					
33876698	11	75	dep	glycans	1528:1534	arg1	M3F					1546:1548	M3F	1546:1548	M3F	1546:1548	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	75	dep	glycans	1528:1534	arg1	M2FA					1537:1540	M2FA	1537:1540	M2FA	1537:1540	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33876698	11	75	dep	glycans	1528:1534	arg1	glycans					1528:1534	Paucimannosidic glycans	1512:1534	Paucimannosidic glycans (M2FA and M3F)	1512:1549	Paucimannosidic glycans (M2FA and M3F) and a bulkier tetra-antennary complex glycan were introduced at asparagine residues N197, N212 and N232.					
33403742	0	0	from	Modification	28:39	arg1	Biosynthesis					72:83	Kanamycin Biosynthesis	62:83	Kanamycin Biosynthesis	62:83	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis.					
33403742	1	1	theme	Streptomyces	186:197	arg1	kanamyceticus					199:211	Streptomyces kanamyceticus	186:211	Streptomyces kanamyceticus	186:211	Kanamycin A is the major 2-deoxystreptamine (2DOS)-containing aminoglycoside antibiotic produced by Streptomyces kanamyceticus.					
33403742	4	2	theme	C-6-NH2	575:581	arg1	introduction					559:570	the introduction	555:570	the introduction of C-6-NH2	555:581	A dehydrogenase, KanQ, and an aminotransferase, KanB, are presumed to be responsible for the introduction of C-6-NH2 , although the substrates have not been identified.					
33403742	7	3	theme	GlcN	940:943	arg1	moiety					945:950	the GlcN moiety	936:950	the GlcN moiety	936:950	Furthermore, the substrate specificity of an oxygenase, KanJ, that is responsible for deamination at C-2 of the GlcN moiety was examined, and the crystal structure of KanJ was determined.					
33403742	5	4	theme	substrate	657:665	arg1	specificity					667:677	the substrate specificity	653:677	the substrate specificity of KanQ	653:685	Here, we examined the substrate specificity of KanQ to better understand the biosynthetic pathway.					
33403742	6	5	theme	3"-deamino	-1:8	arg1	derivative					816:825	the 3''-deamino derivative	800:825	the 3''-deamino derivative	800:825	It was found that KanQ oxidized kanamycin C more efficiently than the 3''-deamino derivative.					
33403742	3	6	from	C-2	400:402	arg1	introduction					408:419	introduction	408:419	introduction of C-6-NH2	408:430	Because the 6ADG moiety is derived from d-glucosamine (GlcN), deamination at C-2 and introduction of C-6-NH2 are required in the biosynthesis.					
33403742	3	6	from	C-2	400:402	arg1	deamination					385:395	deamination	385:395	deamination at C-2	385:402	Because the 6ADG moiety is derived from d-glucosamine (GlcN), deamination at C-2 and introduction of C-6-NH2 are required in the biosynthesis.					
33403742	7	7	theme	moiety	945:950	arg1	deamination					914:924	deamination	914:924	deamination at C-2 of the GlcN moiety	914:950	Furthermore, the substrate specificity of an oxygenase, KanJ, that is responsible for deamination at C-2 of the GlcN moiety was examined, and the crystal structure of KanJ was determined.					
33403742	7	8	theme	oxygenase	873:881	arg1	specificity					855:865	the substrate specificity	841:865	the substrate specificity of an oxygenase, KanJ, that is responsible for deamination at C-2 of the GlcN moiety	841:950	Furthermore, the substrate specificity of an oxygenase, KanJ, that is responsible for deamination at C-2 of the GlcN moiety was examined, and the crystal structure of KanJ was determined.					
33403742	2	9	attach	linked	233:238	arg3	3-amino-3-deoxy-d-glucose					289:313	3-amino-3-deoxy-d-glucose	289:313	3-amino-3-deoxy-d-glucose	289:313	The 2DOS moiety is linked with 6-amino-6-deoxy-d-glucose (6ADG) at O-4 and 3-amino-3-deoxy-d-glucose at O-6.					
33403742	2	9	attach	linked	233:238	arg2	moiety					223:228	The 2DOS moiety	214:228	The 2DOS moiety	214:228	The 2DOS moiety is linked with 6-amino-6-deoxy-d-glucose (6ADG) at O-4 and 3-amino-3-deoxy-d-glucose at O-6.					
33403742	2	9	attach	linked	233:238	arg3	O-4					281:283	O-4	281:283	O-4	281:283	The 2DOS moiety is linked with 6-amino-6-deoxy-d-glucose (6ADG) at O-4 and 3-amino-3-deoxy-d-glucose at O-6.					
33403742	0	10	theme	Post-glycosylation	9:26	arg1	Modification					28:39	Stepwise Post-glycosylation Modification	0:39	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis	0:83	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis.					
33403742	3	11	attach	derived	350:356	arg1	d-glucosamine					363:375	d-glucosamine	363:375	d-glucosamine (GlcN)	363:382	Because the 6ADG moiety is derived from d-glucosamine (GlcN), deamination at C-2 and introduction of C-6-NH2 are required in the biosynthesis.					
33403742	3	11	attach	derived	350:356	arg2	moiety					340:345	the 6ADG moiety	331:345	the 6ADG moiety	331:345	Because the 6ADG moiety is derived from d-glucosamine (GlcN), deamination at C-2 and introduction of C-6-NH2 are required in the biosynthesis.					
33403742	3	11	attach	derived	350:356	arg1	GlcN					378:381	GlcN	378:381	GlcN	378:381	Because the 6ADG moiety is derived from d-glucosamine (GlcN), deamination at C-2 and introduction of C-6-NH2 are required in the biosynthesis.					
33403742	0	12	theme	Stepwise	0:7	arg1	Modification					28:39	Stepwise Post-glycosylation Modification	0:39	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis	0:83	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis.					
33403742	9	13	theme	C-6-NH2	1209:1215	arg1	introduction					1193:1204	the introduction	1189:1204	the introduction of C-6-NH2 by KanQ/KanB and deamination	1189:1244	Thus, the modification of the GlcN moiety occurs after pseudo-trisaccharide formation, followed by the introduction of C-6-NH2 by KanQ/KanB and deamination at C-2 by KanJ.					
33403742	7	14	theme	substrate	845:853	arg1	specificity					855:865	the substrate specificity	841:865	the substrate specificity of an oxygenase, KanJ, that is responsible for deamination at C-2 of the GlcN moiety	841:950	Furthermore, the substrate specificity of an oxygenase, KanJ, that is responsible for deamination at C-2 of the GlcN moiety was examined, and the crystal structure of KanJ was determined.					
33403742	9	15	theme	GlcN	1120:1123	arg1	moiety					1125:1130	the GlcN moiety	1116:1130	the GlcN moiety	1116:1130	Thus, the modification of the GlcN moiety occurs after pseudo-trisaccharide formation, followed by the introduction of C-6-NH2 by KanQ/KanB and deamination at C-2 by KanJ.					
33403742	2	16	theme	2DOS	218:221	arg1	moiety					223:228	The 2DOS moiety	214:228	The 2DOS moiety	214:228	The 2DOS moiety is linked with 6-amino-6-deoxy-d-glucose (6ADG) at O-4 and 3-amino-3-deoxy-d-glucose at O-6.					
33403742	0	17	theme	Moieties	50:57	arg1	Modification					28:39	Stepwise Post-glycosylation Modification	0:39	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis	0:83	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis.					
33403742	4	18	theme	A	466:466	arg1	dehydrogenase					468:480	A dehydrogenase	466:480	A dehydrogenase	466:480	A dehydrogenase, KanQ, and an aminotransferase, KanB, are presumed to be responsible for the introduction of C-6-NH2 , although the substrates have not been identified.					
33403742	0	19	theme	Sugar	44:48	arg1	Moieties					50:57	Sugar Moieties	44:57	Sugar Moieties	44:57	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis.					
33403742	0	20	mod	Modification	28:39	arg1	Moieties					50:57	Sugar Moieties	44:57	Sugar Moieties	44:57	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis.					
33403742	0	20	mod	Modification	28:39	arg3	Post-glycosylation					9:26	Stepwise Post-glycosylation Modification	0:39	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis	0:83	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis.					
33403742	5	21	theme	biosynthetic	712:723	arg1	pathway					725:731	the biosynthetic pathway	708:731	the biosynthetic pathway	708:731	Here, we examined the substrate specificity of KanQ to better understand the biosynthetic pathway.					
33403742	7	22	theme	KanJ	995:998	arg1	structure					982:990	the crystal structure	970:990	the crystal structure of KanJ	970:998	Furthermore, the substrate specificity of an oxygenase, KanJ, that is responsible for deamination at C-2 of the GlcN moiety was examined, and the crystal structure of KanJ was determined.					
33403742	0	23	theme	Kanamycin	62:70	arg1	Biosynthesis					72:83	Kanamycin Biosynthesis	62:83	Kanamycin Biosynthesis	62:83	Stepwise Post-glycosylation Modification of Sugar Moieties in Kanamycin Biosynthesis.					
33403742	3	24	theme	C-6-NH2	424:430	arg1	introduction					408:419	introduction	408:419	introduction of C-6-NH2	408:430	Because the 6ADG moiety is derived from d-glucosamine (GlcN), deamination at C-2 and introduction of C-6-NH2 are required in the biosynthesis.					
33403742	3	24	theme	C-6-NH2	424:430	arg1	deamination					385:395	deamination	385:395	deamination at C-2	385:402	Because the 6ADG moiety is derived from d-glucosamine (GlcN), deamination at C-2 and introduction of C-6-NH2 are required in the biosynthesis.					
33403742	7	25	from	C-2	929:931	arg1	deamination					914:924	deamination	914:924	deamination at C-2 of the GlcN moiety	914:950	Furthermore, the substrate specificity of an oxygenase, KanJ, that is responsible for deamination at C-2 of the GlcN moiety was examined, and the crystal structure of KanJ was determined.					
33403742	2	26	from	O-6	318:320	arg1	O-4					281:283	O-4	281:283	O-4	281:283	The 2DOS moiety is linked with 6-amino-6-deoxy-d-glucose (6ADG) at O-4 and 3-amino-3-deoxy-d-glucose at O-6.					
33403742	2	26	from	O-6	318:320	arg1	3-amino-3-deoxy-d-glucose					289:313	3-amino-3-deoxy-d-glucose	289:313	3-amino-3-deoxy-d-glucose	289:313	The 2DOS moiety is linked with 6-amino-6-deoxy-d-glucose (6ADG) at O-4 and 3-amino-3-deoxy-d-glucose at O-6.					
33403742	5	27	theme	KanQ	682:685	arg1	specificity					667:677	the substrate specificity	653:677	the substrate specificity of KanQ	653:685	Here, we examined the substrate specificity of KanQ to better understand the biosynthetic pathway.					
33403742	9	28	theme	pseudo-trisaccharide	1145:1164	arg1	formation					1166:1174	pseudo-trisaccharide formation	1145:1174	pseudo-trisaccharide formation	1145:1174	Thus, the modification of the GlcN moiety occurs after pseudo-trisaccharide formation, followed by the introduction of C-6-NH2 by KanQ/KanB and deamination at C-2 by KanJ.					
33403742	3	29	theme	6ADG	335:338	arg1	moiety					340:345	the 6ADG moiety	331:345	the 6ADG moiety	331:345	Because the 6ADG moiety is derived from d-glucosamine (GlcN), deamination at C-2 and introduction of C-6-NH2 are required in the biosynthesis.					
33403742	9	30	dep	occurs	1132:1137	arg1	followed					1177:1184	followed	1177:1184	followed by the introduction of C-6-NH2 by KanQ/KanB and deamination at C-2 by KanJ	1177:1259	Thus, the modification of the GlcN moiety occurs after pseudo-trisaccharide formation, followed by the introduction of C-6-NH2 by KanQ/KanB and deamination at C-2 by KanJ.					
33403742	7	31	theme	crystal	974:980	arg1	structure					982:990	the crystal structure	970:990	the crystal structure of KanJ	970:998	Furthermore, the substrate specificity of an oxygenase, KanJ, that is responsible for deamination at C-2 of the GlcN moiety was examined, and the crystal structure of KanJ was determined.					
33403742	9	32	theme	moiety	1125:1130	arg1	modification					1100:1111	the modification	1096:1111	the modification of the GlcN moiety	1096:1130	Thus, the modification of the GlcN moiety occurs after pseudo-trisaccharide formation, followed by the introduction of C-6-NH2 by KanQ/KanB and deamination at C-2 by KanJ.					
33403742	8	33	theme	substrate	1059:1067	arg1	recognition					1069:1079	substrate recognition	1059:1079	substrate recognition by KanJ	1059:1087	It was found that C-6-NH2 is important for substrate recognition by KanJ.					
32873758	11	0	theme	novel	2296:2300	arg1	compounds					2302:2310	novel compounds	2296:2310	novel compounds	2296:2310	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	11	0	theme	novel	2296:2300	arg1	molecules					2221:2229	the first sugar molecules	2205:2229	the first sugar molecules (linking sugars) in these anchors	2205:2263	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	0	1	with	Terminus	66:73	arg1	Sugar					96:100	a Novel Linking Sugar	80:100	a Novel Linking Sugar of the Wbp/Vim Pathway	80:123	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	6	2	theme	T.	1222:1223	arg1	gene					1239:1242	the T. forsythiavimA gene	1218:1242	the T. forsythiavimA gene	1218:1242	In addition, the P. gingivalisvimA mutant which lacks A-LPS was successfully complemented by the T. forsythiavimA gene; however, the linking sugar was altered to include glycine rather than serine.					
32873758	4	3	theme	mass	589:592	arg1	spectrometry					594:605	tandem mass spectrometry	582:605	tandem mass spectrometry	582:605	The residual modifications were then extensively analyzed by tandem mass spectrometry.					
32873758	6	4	theme	linking	1258:1264	arg1	sugar					1266:1270	the linking sugar	1254:1270	the linking sugar	1254:1270	In addition, the P. gingivalisvimA mutant which lacks A-LPS was successfully complemented by the T. forsythiavimA gene; however, the linking sugar was altered to include glycine rather than serine.					
32873758	1	5	theme	cell	242:245	arg1	surface					247:253	the cell surface	238:253	the cell surface where they are anchored via glycolipids	238:293	Porphyromonas gingivalis and Tannerella forsythia use the type IX secretion system to secrete cargo proteins to the cell surface where they are anchored via glycolipids.					
32873758	9	6	theme	key	1640:1642	arg1	compound					1644:1651	the key compound	1636:1651	the key compound that enables the attachment of cargo proteins in P. gingivalis	1636:1714	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	6	theme	key	1640:1642	arg1	sugar					1617:1621	The linking sugar	1605:1621	The linking sugar	1605:1621	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	7	theme	severe	1914:1919	arg1	disease					1925:1931	severe gum disease	1914:1931	severe gum disease	1914:1931	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	8	dep	pathway.IMPORTANCEPorphyromonas	1815:1845	arg1	gingivalis					1847:1856	gingivalis	1847:1856	gingivalis	1847:1856	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	8	dep	pathway.IMPORTANCEPorphyromonas	1815:1845	arg1	forsythia					1873:1881	Tannerella forsythia	1862:1881	Tannerella forsythia	1862:1881	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	8	dep	pathway.IMPORTANCEPorphyromonas	1815:1845	arg1	pathway.IMPORTANCEPorphyromonas					1815:1845	the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia	1803:1881	the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia	1803:1881	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	1	9	theme	Tannerella	155:164	arg1	forsythia					166:174	Tannerella forsythia	155:174	Tannerella forsythia	155:174	Porphyromonas gingivalis and Tannerella forsythia use the type IX secretion system to secrete cargo proteins to the cell surface where they are anchored via glycolipids.					
32873758	5	10	dep	2-N-glycyl	771:780	arg1	3-N-acetylmannuronic					783:802	3-N-acetylmannuronic	783:802	3-N-acetylmannuronic	783:802	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	0	11	theme	Pathway	117:123	arg1	Sugar					96:100	a Novel Linking Sugar	80:100	a Novel Linking Sugar of the Wbp/Vim Pathway	80:123	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	5	12	theme	cargo	631:635	arg1	protein					637:643	each cargo protein	626:643	each cargo protein	626:643	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	5	13	theme	pathway	877:883	arg1	involvement					854:864	the involvement	850:864	the involvement of the Wbp pathway	850:883	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	10	14	theme	glycolipid	2114:2123	arg1	anchors					2125:2131	glycolipid anchors	2114:2131	glycolipid anchors that have remained unidentified for more than 2 decades	2114:2187	The proteins are tethered to the outer membrane via glycolipid anchors that have remained unidentified for more than 2 decades.					
32873758	5	15	dep	gingivalis	1005:1014	arg1	gene					1025:1028	The wbpS gene	1016:1028	P. gingivalis The wbpS gene	1002:1028	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	1	16	theme	type	184:187	arg1	system					202:207	the type IX secretion system	180:207	the type IX secretion system	180:207	Porphyromonas gingivalis and Tannerella forsythia use the type IX secretion system to secrete cargo proteins to the cell surface where they are anchored via glycolipids.					
32873758	14	17	theme	novel	2726:2730	arg1	therapies					2732:2740	novel therapies	2726:2740	novel therapies	2726:2740	Since the cell surface attachment of virulence factors is essential for virulence, these findings reveal new targets for the development of novel therapies.					
32873758	9	18	theme	type	1942:1945	arg1	system					1960:1965	the type IX secretion system	1938:1965	the type IX secretion system (T9SS)	1938:1972	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	18	theme	type	1942:1945	arg1	T9SS					1968:1971	T9SS	1968:1971	T9SS	1968:1971	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	1	19	theme	secretion	192:200	arg1	system					202:207	the type IX secretion system	180:207	the type IX secretion system	180:207	Porphyromonas gingivalis and Tannerella forsythia use the type IX secretion system to secrete cargo proteins to the cell surface where they are anchored via glycolipids.					
32873758	9	20	theme	proteins	1690:1697	arg1	attachment					1670:1679	the attachment	1666:1679	the attachment of cargo proteins in P. gingivalis	1666:1714	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	7	21	theme	group	1351:1355	arg1	removal					1329:1335	removal	1329:1335	removal of the acetyl group at C-2 by the putative deacetylase, VimE	1329:1396	After removal of the acetyl group at C-2 by the putative deacetylase, VimE, VimA presumably transfers the amino acid to complete the biosynthesis.					
32873758	9	22	theme	secretion	1950:1958	arg1	system					1960:1965	the type IX secretion system	1938:1965	the type IX secretion system (T9SS)	1938:1972	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	22	theme	secretion	1950:1958	arg1	T9SS					1968:1971	T9SS	1968:1971	T9SS	1968:1971	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	5	23	theme	linking	667:673	arg1	sugar					675:679	a linking sugar	665:679	a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide	665:1122	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	9	24	theme	P.	1702:1703	arg1	gingivalis					1705:1714	P. gingivalis	1702:1714	P. gingivalis	1702:1714	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	5	25	dep	form	980:983	arg1	identified					1034:1043	identified	1034:1043	was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide	1030:1122	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	11	26	from	molecules	2221:2229	arg1	anchors					2257:2263	these anchors	2251:2263	these anchors	2251:2263	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	14	27	theme	new	2691:2693	arg1	targets					2695:2701	new targets	2691:2701	new targets for the development of novel therapies	2691:2740	Since the cell surface attachment of virulence factors is essential for virulence, these findings reveal new targets for the development of novel therapies.					
32873758	5	28	theme	P.	753:754	arg1	gingivalis					756:765	P. gingivalis	753:765	P. gingivalis	753:765	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	9	29	theme	biosynthetic	1782:1793	arg1	pathway					1795:1801	this novel linking sugar biosynthetic pathway	1757:1801	this novel linking sugar biosynthetic pathway	1757:1801	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	0	30	theme	C	64:64	arg1	Terminus					66:73	the C Terminus	60:73	the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway	60:123	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	13	31	theme	linking	2533:2539	arg1	sugars					2541:2546	similar linking sugars	2525:2546	similar linking sugars	2525:2546	A diverse range of bacteria that do not have the T9SS were found to have the genes for this pathway, suggesting that they may synthesize similar linking sugars for utilization in different systems.					
32873758	7	32	from	removal	1329:1335	arg1	C-2					1360:1362	C-2	1360:1362	C-2	1360:1362	After removal of the acetyl group at C-2 by the putative deacetylase, VimE, VimA presumably transfers the amino acid to complete the biosynthesis.					
32873758	9	33	from	attachment	1670:1679	arg1	gingivalis					1705:1714	P. gingivalis	1702:1714	P. gingivalis	1702:1714	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	3	34	theme	Modified	398:405	arg1	proteins					413:420	Modified cargo proteins	398:420	Modified cargo proteins	398:420	Modified cargo proteins were deglycosylated using trifluoromethanesulfonic acid and digested with trypsin or proteinase K.					
32873758	0	35	theme	Novel	82:86	arg1	Sugar					96:100	a Novel Linking Sugar	80:100	a Novel Linking Sugar of the Wbp/Vim Pathway	80:123	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	5	36	theme	sugar	993:997	arg1	form					980:983	the uronamide form	966:983	the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide	966:1122	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	9	37	theme	novel	1762:1766	arg1	pathway					1795:1801	this novel linking sugar biosynthetic pathway	1757:1801	this novel linking sugar biosynthetic pathway	1757:1801	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	8	38	theme	enzyme	1495:1500	arg1	activities					1502:1511	all the enzyme activities	1487:1511	all the enzyme activities required for the biosynthesis of the linking sugar accounting for six A-LPS-specific genes	1487:1602	The data explain all the enzyme activities required for the biosynthesis of the linking sugar accounting for six A-LPS-specific genes.					
32873758	9	39	theme	virulence	2012:2020	arg1	proteins					2029:2036	virulence factor proteins	2012:2036	virulence factor proteins	2012:2036	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	0	40	theme	Type	0:3	arg1	System					18:23	Type IX Secretion System	0:23	Type IX Secretion System Cargo Proteins	0:38	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	3	41	dep	trypsin	496:502	arg1	K					518:518	K	518:518	K	518:518	Modified cargo proteins were deglycosylated using trifluoromethanesulfonic acid and digested with trypsin or proteinase K.					
32873758	9	42	theme	sugar	1776:1780	arg1	pathway					1795:1801	this novel linking sugar biosynthetic pathway	1757:1801	this novel linking sugar biosynthetic pathway	1757:1801	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	12	43	theme	novel	2317:2321	arg1	pathway					2336:2342	The novel biosynthetic pathway	2313:2342	The novel biosynthetic pathway of these linking sugars	2313:2366	The novel biosynthetic pathway of these linking sugars is also elucidated.					
32873758	9	44	theme	proteins	2029:2036	arg1	arrays					2002:2007	toxic arrays	1996:2007	toxic arrays of virulence factor proteins	1996:2036	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	2	45	theme	known	381:385	arg1	structure					387:395	partially known structure	371:395	partially known structure	371:395	In P. gingivalis, the glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure.					
32873758	0	46	theme	Secretion	8:16	arg1	System					18:23	Type IX Secretion System	0:23	Type IX Secretion System Cargo Proteins	0:38	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	5	47	theme	uronamide	970:978	arg1	form					980:983	the uronamide form	966:983	the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide	966:1122	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	7	48	from	C-2	1360:1362	arg1	removal					1329:1335	removal	1329:1335	removal of the acetyl group at C-2 by the putative deacetylase, VimE	1329:1396	After removal of the acetyl group at C-2 by the putative deacetylase, VimE, VimA presumably transfers the amino acid to complete the biosynthesis.					
32873758	7	48	from	C-2	1360:1362	arg1	group					1351:1355	the acetyl group	1340:1355	the acetyl group at C-2	1340:1362	After removal of the acetyl group at C-2 by the putative deacetylase, VimE, VimA presumably transfers the amino acid to complete the biosynthesis.					
32873758	11	49	theme	linking	2232:2238	arg1	molecules					2221:2229	the first sugar molecules	2205:2229	the first sugar molecules (linking sugars) in these anchors	2205:2263	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	11	49	theme	linking	2232:2238	arg1	sugars					2240:2245	linking sugars	2232:2245	linking sugars	2232:2245	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	0	50	theme	Cargo	25:29	arg1	Proteins					31:38	Type IX Secretion System Cargo Proteins	0:38	Type IX Secretion System Cargo Proteins	0:38	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	14	51	theme	factors	2633:2639	arg1	essential					2644:2652	essential	2644:2652	essential	2644:2652	Since the cell surface attachment of virulence factors is essential for virulence, these findings reveal new targets for the development of novel therapies.					
32873758	14	51	theme	factors	2633:2639	arg1	attachment					2609:2618	the cell surface attachment	2592:2618	the cell surface attachment of virulence factors	2592:2639	Since the cell surface attachment of virulence factors is essential for virulence, these findings reveal new targets for the development of novel therapies.					
32873758	9	52	theme	Wbp/Vim	1807:1813	arg1	gingivalis					1847:1856	gingivalis	1847:1856	gingivalis	1847:1856	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	52	theme	Wbp/Vim	1807:1813	arg1	forsythia					1873:1881	Tannerella forsythia	1862:1881	Tannerella forsythia	1862:1881	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	52	theme	Wbp/Vim	1807:1813	arg1	pathogens					1888:1896	two pathogens	1884:1896	two pathogens associated with severe gum disease	1884:1931	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	52	theme	Wbp/Vim	1807:1813	arg1	pathway.IMPORTANCEPorphyromonas					1815:1845	the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia	1803:1881	the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia	1803:1881	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	12	53	theme	linking	2353:2359	arg1	sugars					2361:2366	these linking sugars	2347:2366	these linking sugars	2347:2366	The novel biosynthetic pathway of these linking sugars is also elucidated.					
32873758	9	54	attach	attachment	1670:1679	arg3	gingivalis					1705:1714	P. gingivalis	1702:1714	P. gingivalis	1702:1714	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	54	attach	attachment	1670:1679	arg2	proteins					1690:1697	cargo proteins	1684:1697	cargo proteins	1684:1697	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	8	55	theme	linking	1550:1556	arg1	sugar					1558:1562	the linking sugar	1546:1562	the linking sugar accounting for six A-LPS-specific genes	1546:1602	The data explain all the enzyme activities required for the biosynthesis of the linking sugar accounting for six A-LPS-specific genes.					
32873758	2	56	theme	P.	299:300	arg1	gingivalis					302:311	P. gingivalis	299:311	P. gingivalis	299:311	In P. gingivalis, the glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure.					
32873758	13	57	from	utilization	2552:2562	arg1	systems					2577:2583	different systems	2567:2583	different systems	2567:2583	A diverse range of bacteria that do not have the T9SS were found to have the genes for this pathway, suggesting that they may synthesize similar linking sugars for utilization in different systems.					
32873758	5	58	theme	P.	1002:1003	arg1	gingivalis					1005:1014	P. gingivalis The wbpS gene	1002:1028	P. gingivalis The wbpS gene	1002:1028	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	5	58	theme	P.	1002:1003	arg1	PGN_1234					1048:1055	PGN_1234	1048:1055	PGN_1234	1048:1055	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	14	59	theme	surface	2601:2607	arg1	essential					2644:2652	essential	2644:2652	essential	2644:2652	Since the cell surface attachment of virulence factors is essential for virulence, these findings reveal new targets for the development of novel therapies.					
32873758	14	59	theme	surface	2601:2607	arg1	attachment					2609:2618	the cell surface attachment	2592:2618	the cell surface attachment of virulence factors	2592:2639	Since the cell surface attachment of virulence factors is essential for virulence, these findings reveal new targets for the development of novel therapies.					
32873758	9	60	theme	Tannerella	1862:1871	arg1	forsythia					1873:1881	Tannerella forsythia	1862:1881	Tannerella forsythia	1862:1881	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	60	theme	Tannerella	1862:1871	arg1	pathway.IMPORTANCEPorphyromonas					1815:1845	the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia	1803:1881	the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia	1803:1881	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	11	61	theme	sugar	2215:2219	arg1	compounds					2302:2310	novel compounds	2296:2310	novel compounds	2296:2310	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	11	61	theme	sugar	2215:2219	arg1	molecules					2221:2229	the first sugar molecules	2205:2229	the first sugar molecules (linking sugars) in these anchors	2205:2263	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	11	61	theme	sugar	2215:2219	arg1	sugars					2240:2245	linking sugars	2232:2245	linking sugars	2232:2245	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	2	62	theme	anionic	332:338	arg1	A-LPS					360:364	A-LPS	360:364	A-LPS	360:364	In P. gingivalis, the glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure.					
32873758	2	62	theme	anionic	332:338	arg1	glycolipid					318:327	the glycolipid	314:327	the glycolipid	314:327	In P. gingivalis, the glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure.					
32873758	2	62	theme	anionic	332:338	arg1	lipopolysaccharide					340:357	anionic lipopolysaccharide	332:357	anionic lipopolysaccharide (A-LPS)	332:365	In P. gingivalis, the glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure.					
32873758	9	63	theme	linking	1609:1615	arg1	compound					1644:1651	the key compound	1636:1651	the key compound that enables the attachment of cargo proteins in P. gingivalis	1636:1714	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	63	theme	linking	1609:1615	arg1	sugar					1617:1621	The linking sugar	1605:1621	The linking sugar	1605:1621	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	5	64	theme	protein	637:643	arg1	terminus					614:621	The C terminus	608:621	The C terminus of each cargo protein	608:643	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	5	64	theme	protein	637:643	arg1	amide-bonded					649:660	amide-bonded	649:660	amide-bonded	649:660	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	7	65	theme	amino	1429:1433	arg1	acid					1435:1438	the amino acid	1425:1438	the amino acid	1425:1438	After removal of the acetyl group at C-2 by the putative deacetylase, VimE, VimA presumably transfers the amino acid to complete the biosynthesis.					
32873758	5	66	theme	2,3-di-N-acetylglucuronic	896:920	arg1	acid					922:925	2,3-di-N-acetylglucuronic acid	896:925	2,3-di-N-acetylglucuronic acid	896:925	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	10	67	theme	outer	2095:2099	arg1	membrane					2101:2108	the outer membrane	2091:2108	the outer membrane	2091:2108	The proteins are tethered to the outer membrane via glycolipid anchors that have remained unidentified for more than 2 decades.					
32873758	4	68	theme	tandem	582:587	arg1	spectrometry					594:605	tandem mass spectrometry	582:605	tandem mass spectrometry	582:605	The residual modifications were then extensively analyzed by tandem mass spectrometry.					
32873758	9	69	theme	gum	1921:1923	arg1	disease					1925:1931	severe gum disease	1914:1931	severe gum disease	1914:1931	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	8	70	theme	sugar	1558:1562	arg1	biosynthesis					1530:1541	the biosynthesis	1526:1541	the biosynthesis of the linking sugar accounting for six A-LPS-specific genes	1526:1602	The data explain all the enzyme activities required for the biosynthesis of the linking sugar accounting for six A-LPS-specific genes.					
32873758	5	71	theme	Wbp	873:875	arg1	pathway					877:883	the Wbp pathway	869:883	the Wbp pathway	869:883	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	6	72	theme	P.	1142:1143	arg1	mutant					1160:1165	the P. gingivalisvimA mutant	1138:1165	the P. gingivalisvimA mutant which lacks A-LPS	1138:1183	In addition, the P. gingivalisvimA mutant which lacks A-LPS was successfully complemented by the T. forsythiavimA gene; however, the linking sugar was altered to include glycine rather than serine.					
32873758	6	73	dep	P.	1142:1143	arg1	gingivalisvimA					1145:1158	gingivalisvimA	1145:1158	gingivalisvimA	1145:1158	In addition, the P. gingivalisvimA mutant which lacks A-LPS was successfully complemented by the T. forsythiavimA gene; however, the linking sugar was altered to include glycine rather than serine.					
32873758	7	74	theme	putative	1371:1378	arg1	deacetylase					1380:1390	the putative deacetylase	1367:1390	the putative deacetylase	1367:1390	After removal of the acetyl group at C-2 by the putative deacetylase, VimE, VimA presumably transfers the amino acid to complete the biosynthesis.					
32873758	7	74	theme	putative	1371:1378	arg1	VimE					1393:1396	VimE	1393:1396	VimE	1393:1396	After removal of the acetyl group at C-2 by the putative deacetylase, VimE, VimA presumably transfers the amino acid to complete the biosynthesis.					
32873758	0	75	theme	Wbp/Vim	109:115	arg1	Pathway					117:123	the Wbp/Vim Pathway	105:123	the Wbp/Vim Pathway	105:123	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	5	76	from	3-N-acetylglucuronamide	726:748	arg1	gingivalis					756:765	P. gingivalis	753:765	P. gingivalis	753:765	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	5	76	from	3-N-acetylglucuronamide	726:748	arg1	forsythia					815:823	T. forsythia	812:823	T. forsythia	812:823	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	1	77	theme	IX	189:190	arg1	system					202:207	the type IX secretion system	180:207	the type IX secretion system	180:207	Porphyromonas gingivalis and Tannerella forsythia use the type IX secretion system to secrete cargo proteins to the cell surface where they are anchored via glycolipids.					
32873758	9	78	theme	cargo	1684:1688	arg1	proteins					1690:1697	cargo proteins	1684:1697	cargo proteins	1684:1697	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	6	79	theme	forsythiavimA	1225:1237	arg1	gene					1239:1242	the T. forsythiavimA gene	1218:1242	the T. forsythiavimA gene	1218:1242	In addition, the P. gingivalisvimA mutant which lacks A-LPS was successfully complemented by the T. forsythiavimA gene; however, the linking sugar was altered to include glycine rather than serine.					
32873758	9	80	theme	IX	1947:1948	arg1	system					1960:1965	the type IX secretion system	1938:1965	the type IX secretion system (T9SS)	1938:1972	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	80	theme	IX	1947:1948	arg1	T9SS					1968:1971	T9SS	1968:1971	T9SS	1968:1971	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	81	dep	designate	1747:1755	arg1	use					1934:1936	use	1934:1936	use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces	1934:2059	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	5	82	from	2-N-seryl	715:723	arg1	gingivalis					756:765	P. gingivalis	753:765	P. gingivalis	753:765	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	5	82	from	2-N-seryl	715:723	arg1	forsythia					815:823	T. forsythia	812:823	T. forsythia	812:823	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	13	83	theme	bacteria	2407:2414	arg1	range					2398:2402	A diverse range	2388:2402	A diverse range of bacteria that do not have the T9SS	2388:2440	A diverse range of bacteria that do not have the T9SS were found to have the genes for this pathway, suggesting that they may synthesize similar linking sugars for utilization in different systems.					
32873758	5	84	theme	WbpS	933:936	arg1	amidotransferase					938:953	a WbpS amidotransferase	931:953	a WbpS amidotransferase	931:953	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	5	85	dep	2-N-seryl	715:723	arg1	produce					958:964	produce	958:964	to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide	955:1122	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	5	85	dep	2-N-seryl	715:723	arg1	produce					888:894	produce	888:894	to produce 2,3-di-N-acetylglucuronic acid	885:925	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	7	86	theme	acetyl	1344:1349	arg1	group					1351:1355	the acetyl group	1340:1355	the acetyl group at C-2	1340:1362	After removal of the acetyl group at C-2 by the putative deacetylase, VimE, VimA presumably transfers the amino acid to complete the biosynthesis.					
32873758	3	87	theme	trifluoromethanesulfonic	448:471	arg1	acid					473:476	trifluoromethanesulfonic acid	448:476	trifluoromethanesulfonic acid	448:476	Modified cargo proteins were deglycosylated using trifluoromethanesulfonic acid and digested with trypsin or proteinase K.					
32873758	13	88	theme	different	2567:2575	arg1	systems					2577:2583	different systems	2567:2583	different systems	2567:2583	A diverse range of bacteria that do not have the T9SS were found to have the genes for this pathway, suggesting that they may synthesize similar linking sugars for utilization in different systems.					
32873758	5	89	from	acid	804:807	arg1	gingivalis					756:765	P. gingivalis	753:765	P. gingivalis	753:765	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	5	89	from	acid	804:807	arg1	forsythia					815:823	T. forsythia	812:823	T. forsythia	812:823	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	1	90	theme	Porphyromonas	126:138	arg1	gingivalis					140:149	Porphyromonas gingivalis	126:149	Porphyromonas gingivalis	126:149	Porphyromonas gingivalis and Tannerella forsythia use the type IX secretion system to secrete cargo proteins to the cell surface where they are anchored via glycolipids.					
32873758	13	91	theme	similar	2525:2531	arg1	sugars					2541:2546	similar linking sugars	2525:2546	similar linking sugars	2525:2546	A diverse range of bacteria that do not have the T9SS were found to have the genes for this pathway, suggesting that they may synthesize similar linking sugars for utilization in different systems.					
32873758	14	92	theme	therapies	2732:2740	arg1	development					2711:2721	the development	2707:2721	the development of novel therapies	2707:2740	Since the cell surface attachment of virulence factors is essential for virulence, these findings reveal new targets for the development of novel therapies.					
32873758	11	93	theme	first	2209:2213	arg1	compounds					2302:2310	novel compounds	2296:2310	novel compounds	2296:2310	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	11	93	theme	first	2209:2213	arg1	molecules					2221:2229	the first sugar molecules	2205:2229	the first sugar molecules (linking sugars) in these anchors	2205:2263	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	11	93	theme	first	2209:2213	arg1	sugars					2240:2245	linking sugars	2232:2245	linking sugars	2232:2245	In this study, the first sugar molecules (linking sugars) in these anchors are identified and found to be novel compounds.					
32873758	9	94	theme	toxic	1996:2000	arg1	arrays					2002:2007	toxic arrays	1996:2007	toxic arrays of virulence factor proteins	1996:2036	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	0	95	theme	Linking	88:94	arg1	Sugar					96:100	a Novel Linking Sugar	80:100	a Novel Linking Sugar of the Wbp/Vim Pathway	80:123	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	5	96	theme	C	612:612	arg1	terminus					614:621	The C terminus	608:621	The C terminus of each cargo protein	608:643	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	5	96	theme	C	612:612	arg1	amide-bonded					649:660	amide-bonded	649:660	amide-bonded	649:660	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	12	97	theme	biosynthetic	2323:2334	arg1	pathway					2336:2342	The novel biosynthetic pathway	2313:2342	The novel biosynthetic pathway of these linking sugars	2313:2366	The novel biosynthetic pathway of these linking sugars is also elucidated.					
32873758	3	98	theme	cargo	407:411	arg1	proteins					413:420	Modified cargo proteins	398:420	Modified cargo proteins	398:420	Modified cargo proteins were deglycosylated using trifluoromethanesulfonic acid and digested with trypsin or proteinase K.					
32873758	0	99	theme	IX	5:6	arg1	System					18:23	Type IX Secretion System	0:23	Type IX Secretion System Cargo Proteins	0:38	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	9	100	theme	linking	1768:1774	arg1	pathway					1795:1801	this novel linking sugar biosynthetic pathway	1757:1801	this novel linking sugar biosynthetic pathway	1757:1801	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	9	101	theme	factor	2022:2027	arg1	proteins					2029:2036	virulence factor proteins	2012:2036	virulence factor proteins	2012:2036	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	0	102	theme	System	18:23	arg1	Proteins					31:38	Type IX Secretion System Cargo Proteins	0:38	Type IX Secretion System Cargo Proteins	0:38	Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway.					
32873758	2	103	theme	structure	387:395	arg1	A-LPS					360:364	A-LPS	360:364	A-LPS	360:364	In P. gingivalis, the glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure.					
32873758	2	103	theme	structure	387:395	arg1	glycolipid					318:327	the glycolipid	314:327	the glycolipid	314:327	In P. gingivalis, the glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure.					
32873758	2	103	theme	structure	387:395	arg1	lipopolysaccharide					340:357	anionic lipopolysaccharide	332:357	anionic lipopolysaccharide (A-LPS)	332:365	In P. gingivalis, the glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure.					
32873758	5	104	theme	2-N-glycyl	771:780	arg1	acid					804:807	2-N-glycyl, 3-N-acetylmannuronic acid	771:807	2-N-glycyl, 3-N-acetylmannuronic acid	771:807	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	5	105	theme	wbpS	1020:1023	arg1	gene					1025:1028	The wbpS gene	1016:1028	P. gingivalis The wbpS gene	1002:1028	The C terminus of each cargo protein was amide-bonded to a linking sugar whose structure was deduced to be 2-N-seryl, 3-N-acetylglucuronamide in P. gingivalis and 2-N-glycyl, 3-N-acetylmannuronic acid in T. forsythia The structures indicated the involvement of the Wbp pathway to produce 2,3-di-N-acetylglucuronic acid and a WbpS amidotransferase to produce the uronamide form of this sugar in P. gingivalis The wbpS gene was identified as PGN_1234 as its deletion resulted in the inability to produce the uronamide.					
32873758	12	106	theme	sugars	2361:2366	arg1	pathway					2336:2342	The novel biosynthetic pathway	2313:2342	The novel biosynthetic pathway of these linking sugars	2313:2366	The novel biosynthetic pathway of these linking sugars is also elucidated.					
32873758	9	107	theme	cell	2047:2050	arg1	surfaces					2052:2059	their cell surfaces	2041:2059	their cell surfaces	2041:2059	The linking sugar is therefore the key compound that enables the attachment of cargo proteins in P. gingivalis and T. forsythia We propose to designate this novel linking sugar biosynthetic pathway the Wbp/Vim pathway.IMPORTANCEPorphyromonas gingivalis and Tannerella forsythia, two pathogens associated with severe gum disease, use the type IX secretion system (T9SS) to secrete and attach toxic arrays of virulence factor proteins to their cell surfaces.					
32873758	3	108	gly	deglycosylated	427:440	arg1	proteins					413:420	Modified cargo proteins	398:420	Modified cargo proteins	398:420	Modified cargo proteins were deglycosylated using trifluoromethanesulfonic acid and digested with trypsin or proteinase K.					
32873758	2	109	from	lipopolysaccharide	340:357	arg1	gingivalis					302:311	P. gingivalis	299:311	P. gingivalis	299:311	In P. gingivalis, the glycolipid is anionic lipopolysaccharide (A-LPS), of partially known structure.					
32873758	14	110	theme	virulence	2623:2631	arg1	factors					2633:2639	virulence factors	2623:2639	virulence factors	2623:2639	Since the cell surface attachment of virulence factors is essential for virulence, these findings reveal new targets for the development of novel therapies.					
32873758	4	111	theme	residual	525:532	arg1	modifications					534:546	The residual modifications	521:546	The residual modifications	521:546	The residual modifications were then extensively analyzed by tandem mass spectrometry.					
32873758	13	112	theme	diverse	2390:2396	arg1	range					2398:2402	A diverse range	2388:2402	A diverse range of bacteria that do not have the T9SS	2388:2440	A diverse range of bacteria that do not have the T9SS were found to have the genes for this pathway, suggesting that they may synthesize similar linking sugars for utilization in different systems.					
32873758	13	113	contain	have	2456:2459	arg1	range					2398:2402	A diverse range	2388:2402	A diverse range of bacteria that do not have the T9SS	2388:2440	A diverse range of bacteria that do not have the T9SS were found to have the genes for this pathway, suggesting that they may synthesize similar linking sugars for utilization in different systems.					
32873758	13	113	contain	have	2456:2459	arg2	genes					2465:2469	the genes	2461:2469	the genes for this pathway	2461:2486	A diverse range of bacteria that do not have the T9SS were found to have the genes for this pathway, suggesting that they may synthesize similar linking sugars for utilization in different systems.					
32873758	14	114	theme	cell	2596:2599	arg1	essential					2644:2652	essential	2644:2652	essential	2644:2652	Since the cell surface attachment of virulence factors is essential for virulence, these findings reveal new targets for the development of novel therapies.					
32873758	14	114	theme	cell	2596:2599	arg1	attachment					2609:2618	the cell surface attachment	2592:2618	the cell surface attachment of virulence factors	2592:2639	Since the cell surface attachment of virulence factors is essential for virulence, these findings reveal new targets for the development of novel therapies.					
32873758	13	115	contain	have	2428:2431	arg1	bacteria					2407:2414	bacteria	2407:2414	bacteria that do not have the T9SS	2407:2440	A diverse range of bacteria that do not have the T9SS were found to have the genes for this pathway, suggesting that they may synthesize similar linking sugars for utilization in different systems.					
32873758	13	115	contain	have	2428:2431	arg2	T9SS					2437:2440	the T9SS	2433:2440	the T9SS	2433:2440	A diverse range of bacteria that do not have the T9SS were found to have the genes for this pathway, suggesting that they may synthesize similar linking sugars for utilization in different systems.					
32873758	8	116	theme	A-LPS-specific	1583:1596	arg1	genes					1598:1602	six A-LPS-specific genes	1579:1602	six A-LPS-specific genes	1579:1602	The data explain all the enzyme activities required for the biosynthesis of the linking sugar accounting for six A-LPS-specific genes.					
32873758	1	117	theme	cargo	220:224	arg1	proteins					226:233	cargo proteins	220:233	cargo proteins	220:233	Porphyromonas gingivalis and Tannerella forsythia use the type IX secretion system to secrete cargo proteins to the cell surface where they are anchored via glycolipids.					
32828279	5	0	theme	Endo-CC	751:757	arg1	N180H					759:763	Endo-CC N180H	751:763	Endo-CC N180H	751:763	The kinetic parameters of Endo-CC N180H towards SGP and pNP-GlcNAc were determined.					
32828279	1	1	theme	flavonoid	194:202	arg1	glycosides					204:213	flavonoid glycosides	194:213	flavonoid glycosides	194:213	Flavonoids are generally glycosylated, and the glycan moieties of flavonoid glycosides are known to greatly affect their physicochemical and biological properties.					
32828279	4	2	theme	sialylglyco	662:672	arg1	peptide					674:680	the sialylglyco peptide	658:680	the sialylglyco peptide	658:680	Endo-CC N180H transferred the sialyl biantennary glycans from the sialylglyco peptide to pNP-GlcNAc and narigenin-7-O-glucoside.					
32828279	0	3	theme	endo-β-N-acetylglucosaminidase	96:125	arg1	mutant					66:71	an N180H mutant	57:71	an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase	57:125	Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase.					
32828279	5	4	theme	N180H	759:763	arg1	parameters					737:746	The kinetic parameters	725:746	The kinetic parameters of Endo-CC N180H towards SGP and pNP-GlcNAc	725:790	The kinetic parameters of Endo-CC N180H towards SGP and pNP-GlcNAc were determined.					
32828279	7	5	theme	biological	1068:1077	arg1	functions					1079:1087	the inherent biological functions	1055:1087	the inherent biological functions of the flavonoids	1055:1105	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	1	6	theme	glycosides	204:213	arg1	moieties					182:189	the glycan moieties	171:189	the glycan moieties of flavonoid glycosides	171:213	Flavonoids are generally glycosylated, and the glycan moieties of flavonoid glycosides are known to greatly affect their physicochemical and biological properties.					
32828279	7	7	theme	functions	1079:1087	arg1	improvement					1040:1050	the improvement	1036:1050	the improvement of the inherent biological functions of the flavonoids	1036:1105	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	7	7	theme	functions	1079:1087	arg1	creation					1111:1118	creation	1111:1118	creation of novel flavonoid glycoside derivatives	1111:1159	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	2	8	theme	tools	330:334	arg1	tools					330:334	tools	330:334	tools for glycan remodeling of flavonoid glycosides	330:380	Thus, the development of a variety of tools for glycan remodeling of flavonoid glycosides is highly desired.					
32828279	2	8	theme	tools	330:334	arg1	variety					319:325	a variety	317:325	a variety of tools for glycan remodeling of flavonoid glycosides	317:380	Thus, the development of a variety of tools for glycan remodeling of flavonoid glycosides is highly desired.					
32828279	3	9	theme	endo-β-N-acetylglucosaminidase	404:433	arg1	N180H					450:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H	401:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H	401:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	3	9	theme	endo-β-N-acetylglucosaminidase	404:433	arg1	tool					507:510	an excellent chemoenzymatic tool	479:510	an excellent chemoenzymatic tool for creating sialylglycoproteins	479:543	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	2	10	theme	flavonoid	361:369	arg1	glycosides					371:380	flavonoid glycosides	361:380	flavonoid glycosides	361:380	Thus, the development of a variety of tools for glycan remodeling of flavonoid glycosides is highly desired.					
32828279	1	11	gly	glycosylated	153:164	arg1	Flavonoids					128:137	Flavonoids	128:137	Flavonoids	128:137	Flavonoids are generally glycosylated, and the glycan moieties of flavonoid glycosides are known to greatly affect their physicochemical and biological properties.					
32828279	6	12	theme	N180H	916:920	arg1	glucosides					819:828	Flavonoid glucosides	809:828	Flavonoid glucosides harboring a 1,3-diol structure in the glucose moieties	809:883	Flavonoid glucosides harboring a 1,3-diol structure in the glucose moieties acted as substrates of Endo-CC N180H.					
32828279	6	12	theme	N180H	916:920	arg1	substrates					894:903	substrates	894:903	substrates of Endo-CC N180H	894:920	Flavonoid glucosides harboring a 1,3-diol structure in the glucose moieties acted as substrates of Endo-CC N180H.					
32828279	2	13	theme	variety	319:325	arg1	development					302:312	the development	298:312	the development of a variety of tools for glycan remodeling of flavonoid glycosides	298:380	Thus, the development of a variety of tools for glycan remodeling of flavonoid glycosides is highly desired.					
32828279	3	14	theme	chemoenzymatic	492:505	arg1	N180H					450:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H	401:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H	401:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	3	14	theme	chemoenzymatic	492:505	arg1	tool					507:510	an excellent chemoenzymatic tool	479:510	an excellent chemoenzymatic tool for creating sialylglycoproteins	479:543	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	5	15	theme	kinetic	729:735	arg1	parameters					737:746	The kinetic parameters	725:746	The kinetic parameters of Endo-CC N180H towards SGP and pNP-GlcNAc	725:790	The kinetic parameters of Endo-CC N180H towards SGP and pNP-GlcNAc were determined.					
32828279	6	16	theme	Endo-CC	908:914	arg1	N180H					916:920	Endo-CC N180H	908:920	Endo-CC N180H	908:920	Flavonoid glucosides harboring a 1,3-diol structure in the glucose moieties acted as substrates of Endo-CC N180H.					
32828279	3	17	gly	glycosylation	567:579	arg1	flavonoids					584:593	flavonoids	584:593	flavonoids	584:593	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	6	18	theme	Flavonoid	809:817	arg1	glucosides					819:828	Flavonoid glucosides	809:828	Flavonoid glucosides harboring a 1,3-diol structure in the glucose moieties	809:883	Flavonoid glucosides harboring a 1,3-diol structure in the glucose moieties acted as substrates of Endo-CC N180H.					
32828279	6	18	theme	Flavonoid	809:817	arg1	substrates					894:903	substrates	894:903	substrates of Endo-CC N180H	894:920	Flavonoid glucosides harboring a 1,3-diol structure in the glucose moieties acted as substrates of Endo-CC N180H.					
32828279	4	19	theme	biantennary	633:643	arg1	glycans					645:651	the sialyl biantennary glycans	622:651	the sialyl biantennary glycans from the sialylglyco peptide	622:680	Endo-CC N180H transferred the sialyl biantennary glycans from the sialylglyco peptide to pNP-GlcNAc and narigenin-7-O-glucoside.					
32828279	7	20	theme	sialyl	944:949	arg1	transfer					970:977	the sialyl biantennary glycan transfer	940:977	the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H	940:1011	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	3	21	theme	excellent	482:490	arg1	N180H					450:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H	401:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H	401:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	3	21	theme	excellent	482:490	arg1	tool					507:510	an excellent chemoenzymatic tool	479:510	an excellent chemoenzymatic tool for creating sialylglycoproteins	479:543	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	3	22	theme	mutant	435:440	arg1	N180H					450:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H	401:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H	401:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	3	22	theme	mutant	435:440	arg1	tool					507:510	an excellent chemoenzymatic tool	479:510	an excellent chemoenzymatic tool for creating sialylglycoproteins	479:543	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	4	23	theme	sialyl	626:631	arg1	glycans					645:651	the sialyl biantennary glycans	622:651	the sialyl biantennary glycans from the sialylglyco peptide	622:680	Endo-CC N180H transferred the sialyl biantennary glycans from the sialylglyco peptide to pNP-GlcNAc and narigenin-7-O-glucoside.					
32828279	7	24	theme	inherent	1059:1066	arg1	functions					1079:1087	the inherent biological functions	1055:1087	the inherent biological functions of the flavonoids	1055:1105	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	2	25	theme	glycan	340:345	arg1	remodeling					347:356	glycan remodeling	340:356	glycan remodeling of flavonoid glycosides	340:380	Thus, the development of a variety of tools for glycan remodeling of flavonoid glycosides is highly desired.					
32828279	7	26	theme	biantennary	951:961	arg1	transfer					970:977	the sialyl biantennary glycan transfer	940:977	the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H	940:1011	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	3	27	theme	Endo-CC	442:448	arg1	N180H					450:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H	401:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H	401:454	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	3	27	theme	Endo-CC	442:448	arg1	tool					507:510	an excellent chemoenzymatic tool	479:510	an excellent chemoenzymatic tool for creating sialylglycoproteins	479:543	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	6	28	from	structure	851:859	arg1	moieties					876:883	the glucose moieties	864:883	the glucose moieties	864:883	Flavonoid glucosides harboring a 1,3-diol structure in the glucose moieties acted as substrates of Endo-CC N180H.					
32828279	0	29	theme	N180H	60:64	arg1	mutant					66:71	an N180H mutant	57:71	an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase	57:125	Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase.					
32828279	7	30	theme	human	1172:1176	arg1	drugs					1214:1218	drugs	1214:1218	drugs	1214:1218	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	7	30	theme	human	1172:1176	arg1	foods					1204:1208	foods	1204:1208	foods	1204:1208	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	7	30	theme	human	1172:1176	arg1	benefits					1185:1192	future human health benefits	1165:1192	future human health benefits including foods and drugs	1165:1218	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	1	31	theme	physicochemical	249:263	arg1	properties					280:289	their physicochemical and biological properties	243:289	their physicochemical and biological properties	243:289	Flavonoids are generally glycosylated, and the glycan moieties of flavonoid glycosides are known to greatly affect their physicochemical and biological properties.					
32828279	7	32	theme	health	1178:1183	arg1	drugs					1214:1218	drugs	1214:1218	drugs	1214:1218	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	7	32	theme	health	1178:1183	arg1	foods					1204:1208	foods	1204:1208	foods	1204:1208	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	7	32	theme	health	1178:1183	arg1	benefits					1185:1192	future human health benefits	1165:1192	future human health benefits including foods and drugs	1165:1218	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	3	33	gly	sialylglycoproteins	525:543	arg1	sialylglycoproteins					525:543	sialylglycoproteins	525:543	sialylglycoproteins	525:543	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	7	34	theme	novel	1123:1127	arg1	derivatives					1149:1159	novel flavonoid glycoside derivatives	1123:1159	novel flavonoid glycoside derivatives	1123:1159	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	4	35	theme	Endo-CC	596:602	arg1	N180H					604:608	Endo-CC N180H	596:608	Endo-CC N180H	596:608	Endo-CC N180H transferred the sialyl biantennary glycans from the sialylglyco peptide to pNP-GlcNAc and narigenin-7-O-glucoside.					
32828279	7	36	theme	flavonoid	1129:1137	arg1	derivatives					1149:1159	novel flavonoid glycoside derivatives	1123:1159	novel flavonoid glycoside derivatives	1123:1159	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	7	37	theme	future	1165:1170	arg1	drugs					1214:1218	drugs	1214:1218	drugs	1214:1218	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	7	37	theme	future	1165:1170	arg1	foods					1204:1208	foods	1204:1208	foods	1204:1208	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	7	37	theme	future	1165:1170	arg1	benefits					1185:1192	future human health benefits	1165:1192	future human health benefits including foods and drugs	1165:1218	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	6	38	theme	glucose	868:874	arg1	moieties					876:883	the glucose moieties	864:883	the glucose moieties	864:883	Flavonoid glucosides harboring a 1,3-diol structure in the glucose moieties acted as substrates of Endo-CC N180H.					
32828279	7	39	theme	glycoside	1139:1147	arg1	derivatives					1149:1159	novel flavonoid glycoside derivatives	1123:1159	novel flavonoid glycoside derivatives	1123:1159	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	2	40	theme	glycosides	371:380	arg1	remodeling					347:356	glycan remodeling	340:356	glycan remodeling of flavonoid glycosides	340:380	Thus, the development of a variety of tools for glycan remodeling of flavonoid glycosides is highly desired.					
32828279	1	41	theme	biological	269:278	arg1	properties					280:289	their physicochemical and biological properties	243:289	their physicochemical and biological properties	243:289	Flavonoids are generally glycosylated, and the glycan moieties of flavonoid glycosides are known to greatly affect their physicochemical and biological properties.					
32828279	7	42	theme	derivatives	1149:1159	arg1	improvement					1040:1050	the improvement	1036:1050	the improvement of the inherent biological functions of the flavonoids	1036:1105	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	7	42	theme	derivatives	1149:1159	arg1	creation					1111:1118	creation	1111:1118	creation of novel flavonoid glycoside derivatives	1111:1159	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	7	43	theme	flavonoids	1096:1105	arg1	functions					1079:1087	the inherent biological functions	1055:1087	the inherent biological functions of the flavonoids	1055:1105	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	1	44	theme	glycan	175:180	arg1	moieties					182:189	the glycan moieties	171:189	the glycan moieties of flavonoid glycosides	171:213	Flavonoids are generally glycosylated, and the glycan moieties of flavonoid glycosides are known to greatly affect their physicochemical and biological properties.					
32828279	7	45	theme	glycan	963:968	arg1	transfer					970:977	the sialyl biantennary glycan transfer	940:977	the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H	940:1011	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	0	46	theme	cinerea	88:94	arg1	endo-β-N-acetylglucosaminidase					96:125	Coprinopsis cinerea endo-β-N-acetylglucosaminidase	76:125	Coprinopsis cinerea endo-β-N-acetylglucosaminidase	76:125	Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase.					
32828279	3	47	theme	flavonoids	584:593	arg1	glycosylation					567:579	the glycosylation	563:579	the glycosylation of flavonoids	563:593	An endo-β-N-acetylglucosaminidase mutant Endo-CC N180H, which is developed as an excellent chemoenzymatic tool for creating sialylglycoproteins, was employed for the glycosylation of flavonoids.					
32828279	0	48	theme	Coprinopsis	76:86	arg1	endo-β-N-acetylglucosaminidase					96:125	Coprinopsis cinerea endo-β-N-acetylglucosaminidase	76:125	Coprinopsis cinerea endo-β-N-acetylglucosaminidase	76:125	Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase.					
32828279	6	49	theme	1,3-diol	842:849	arg1	structure					851:859	a 1,3-diol structure	840:859	a 1,3-diol structure in the glucose moieties	840:883	Flavonoid glucosides harboring a 1,3-diol structure in the glucose moieties acted as substrates of Endo-CC N180H.					
32828279	7	50	theme	Endo-CC	999:1005	arg1	N180H					1007:1011	Endo-CC N180H	999:1011	Endo-CC N180H	999:1011	We proposed that the sialyl biantennary glycan transfer to the flavonoid by Endo-CC N180H could pave the way for the improvement of the inherent biological functions of the flavonoids and creation of novel flavonoid glycoside derivatives for future human health benefits including foods and drugs.					
32828279	4	51	from	peptide	674:680	arg1	glycans					645:651	the sialyl biantennary glycans	622:651	the sialyl biantennary glycans from the sialylglyco peptide	622:680	Endo-CC N180H transferred the sialyl biantennary glycans from the sialylglyco peptide to pNP-GlcNAc and narigenin-7-O-glucoside.					
32449946	1	0	theme	reaction	298:305	arg1	temperature					307:317	reaction temperature	298:317	reaction temperature (50 to 90 °C)	298:331	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	0	theme	reaction	298:305	arg1	90 °C					326:330	90 °C	326:330	90 °C	326:330	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	6	1	theme	ability	1206:1212	arg1	index					1183:1187	the emulsion activity index	1161:1187	the emulsion activity index of WPI-D and the ability to encapsulate β-carotene	1161:1238	However, due to the polymerization between the WPI molecules, the emulsion activity index of WPI-D and the ability to encapsulate β-carotene were lowered at 90 °C (P < 0.05).					
32449946	1	2	from	effect	155:160	arg1	structure					205:213	molecular structure	195:213	molecular structure	195:213	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	2	from	effect	155:160	arg1	properties					230:239	functional properties	219:239	functional properties	219:239	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	7	3	theme	emulsifying	1385:1395	arg1	properties					1397:1406	the emulsifying properties	1381:1406	the emulsifying properties	1381:1406	Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate β-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation.					
32449946	8	4	theme	glycosylation	1559:1571	arg1	reaction					1573:1580	The glycosylation reaction	1555:1580	The glycosylation reaction	1555:1580	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	1	5	theme	temperature	307:317	arg1	effect					288:293	the effect	284:293	the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D)	284:418	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	6	from	effect	288:293	arg1	structure					350:358	molecular structure	340:358	molecular structure	340:358	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	6	from	effect	288:293	arg1	properties					375:384	functional properties	364:384	functional properties	364:384	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	7	theme	WPI-dextran	389:399	arg1	WPI-D					413:417	WPI-D	413:417	WPI-D	413:417	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	7	theme	WPI-dextran	389:399	arg1	conjugates					401:410	WPI-dextran conjugates	389:410	WPI-dextran conjugates (WPI-D)	389:418	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	8	theme	whey	244:247	arg1	WPI					266:268	WPI	266:268	WPI	266:268	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	8	theme	whey	244:247	arg1	isolate					257:263	whey protein isolate	244:263	whey protein isolate (WPI)	244:269	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	0	9	from	Effect	0:5	arg1	ability					64:70	the ability	60:70	the ability	60:70	Effect of reaction temperature on the protein structure and the ability to encapsulate β-carotene of WPI-dextran conjugates.					
32449946	0	9	from	Effect	0:5	arg1	structure					46:54	the protein structure	34:54	the protein structure	34:54	Effect of reaction temperature on the protein structure and the ability to encapsulate β-carotene of WPI-dextran conjugates.					
32449946	1	10	theme	conjugates	401:410	arg1	structure					350:358	molecular structure	340:358	molecular structure	340:358	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	10	theme	conjugates	401:410	arg1	properties					375:384	functional properties	364:384	functional properties	364:384	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	7	11	theme	functional	1351:1360	arg1	properties					1362:1371	functional properties	1351:1371	functional properties	1351:1371	Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate β-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation.					
32449946	8	12	theme	WPI-dextran	1740:1750	arg1	conjugates					1752:1761	WPI-dextran conjugates	1740:1761	WPI-dextran conjugates	1740:1761	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	1	13	theme	protein	249:255	arg1	WPI					266:268	WPI	266:268	WPI	266:268	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	13	theme	protein	249:255	arg1	isolate					257:263	whey protein isolate	244:263	whey protein isolate (WPI)	244:269	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	6	14	theme	WPI-D	1192:1196	arg1	index					1183:1187	the emulsion activity index	1161:1187	the emulsion activity index of WPI-D and the ability to encapsulate β-carotene	1161:1238	However, due to the polymerization between the WPI molecules, the emulsion activity index of WPI-D and the ability to encapsulate β-carotene were lowered at 90 °C (P < 0.05).					
32449946	4	15	theme	WPI-D	879:883	arg1	β-carotene					865:874	β-carotene	865:874	β-carotene of WPI-D	865:883	The emulsifying properties and the ability to encapsulate β-carotene of WPI-D were improved compared with WPI (P < 0.05).					
32449946	1	16	theme	isolate	257:263	arg1	structure					205:213	molecular structure	195:213	molecular structure	195:213	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	16	theme	isolate	257:263	arg1	properties					230:239	functional properties	219:239	functional properties	219:239	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	0	17	theme	WPI-dextran	101:111	arg1	conjugates					113:122	WPI-dextran conjugates	101:122	WPI-dextran conjugates	101:122	Effect of reaction temperature on the protein structure and the ability to encapsulate β-carotene of WPI-dextran conjugates.					
32449946	8	18	theme	protein	1651:1657	arg1	isolate					1659:1665	Whey protein isolate	1646:1665	Whey protein isolate	1646:1665	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	4	19	theme	emulsifying	811:821	arg1	properties					823:832	The emulsifying properties	807:832	The emulsifying properties	807:832	The emulsifying properties and the ability to encapsulate β-carotene of WPI-D were improved compared with WPI (P < 0.05).					
32449946	5	20	theme	reaction	938:945	arg1	70					963:964	70	963:964	70	963:964	When the reaction temperature was 70 and 80 °C, the EG and the ability to encapsulate β-carotene of WPI-D were better (P < 0.05), which was related to protein unfolding.					
32449946	5	20	theme	reaction	938:945	arg1	temperature					947:957	the reaction temperature	934:957	the reaction temperature	934:957	When the reaction temperature was 70 and 80 °C, the EG and the ability to encapsulate β-carotene of WPI-D were better (P < 0.05), which was related to protein unfolding.					
32449946	8	21	theme	Whey	1646:1649	arg1	isolate					1659:1665	Whey protein isolate	1646:1665	Whey protein isolate	1646:1665	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	2	22	theme	glycation	450:458	arg1	extent					440:445	the extent	436:445	the extent of glycation (EG) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis	436:525	The results of the extent of glycation (EG) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed the formation of WPI-D.					
32449946	3	23	theme	tryptophan	773:782	arg1	content					784:790	the tryptophan content	769:790	the tryptophan content	769:790	Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the β-sheet content was decreased and the tryptophan content was increased.					
32449946	7	24	theme	WPI	1376:1378	arg1	structure					1337:1345	molecular structure	1327:1345	molecular structure	1327:1345	Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate β-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation.					
32449946	7	24	theme	WPI	1376:1378	arg1	properties					1362:1371	functional properties	1351:1371	functional properties	1351:1371	Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate β-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation.					
32449946	5	25	theme	WPI-D	1029:1033	arg1	β-carotene					1015:1024	β-carotene	1015:1024	β-carotene of WPI-D	1015:1033	When the reaction temperature was 70 and 80 °C, the EG and the ability to encapsulate β-carotene of WPI-D were better (P < 0.05), which was related to protein unfolding.					
32449946	3	26	theme	molecular	672:680	arg1	structure					682:690	the molecular structure	668:690	the molecular structure of WPI	668:697	Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the β-sheet content was decreased and the tryptophan content was increased.					
32449946	6	27	theme	activity	1174:1181	arg1	index					1183:1187	the emulsion activity index	1161:1187	the emulsion activity index of WPI-D and the ability to encapsulate β-carotene	1161:1238	However, due to the polymerization between the WPI molecules, the emulsion activity index of WPI-D and the ability to encapsulate β-carotene were lowered at 90 °C (P < 0.05).					
32449946	8	28	theme	isolate	1659:1665	arg1	structure					1607:1615	molecular structure	1597:1615	molecular structure	1597:1615	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	8	28	theme	isolate	1659:1665	arg1	properties					1632:1641	functional properties	1621:1641	functional properties	1621:1641	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	7	29	theme	reaction	1493:1500	arg1	temperature					1502:1512	the reaction temperature	1489:1512	the reaction temperature of glycosylation	1489:1529	Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate β-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation.					
32449946	3	30	theme	fluorescence	627:638	arg1	spectroscopy					640:651	fluorescence spectroscopy	627:651	fluorescence spectroscopy	627:651	Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the β-sheet content was decreased and the tryptophan content was increased.					
32449946	6	31	theme	emulsion	1165:1172	arg1	index					1183:1187	the emulsion activity index	1161:1187	the emulsion activity index of WPI-D and the ability to encapsulate β-carotene	1161:1238	However, due to the polymerization between the WPI molecules, the emulsion activity index of WPI-D and the ability to encapsulate β-carotene were lowered at 90 °C (P < 0.05).					
32449946	7	32	theme	molecular	1327:1335	arg1	structure					1337:1345	molecular structure	1327:1345	molecular structure	1327:1345	Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate β-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation.					
32449946	8	33	theme	emulsifying	1672:1682	arg1	properties					1684:1693	the emulsifying properties	1668:1693	the emulsifying properties	1668:1693	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	1	34	theme	glycosylation	165:177	arg1	reaction					179:186	glycosylation reaction	165:186	glycosylation reaction	165:186	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	0	35	theme	temperature	19:29	arg1	Effect					0:5	Effect	0:5	Effect of reaction temperature on the protein structure and the ability	0:70	Effect of reaction temperature on the protein structure and the ability to encapsulate β-carotene of WPI-dextran conjugates.					
32449946	2	36	theme	sulfate-polyacrylamide	484:505	arg1	electrophoresis					511:525	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	469:525	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	469:525	The results of the extent of glycation (EG) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed the formation of WPI-D.					
32449946	8	37	theme	reaction	1797:1804	arg1	temperature					1806:1816	the reaction temperature	1793:1816	the reaction temperature of glycosylation	1793:1833	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	1	38	theme	reaction	179:186	arg1	effect					155:160	the effect	151:160	the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI)	151:269	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	0	39	theme	reaction	10:17	arg1	temperature					19:29	reaction temperature	10:29	reaction temperature	10:29	Effect of reaction temperature on the protein structure and the ability to encapsulate β-carotene of WPI-dextran conjugates.					
32449946	3	40	theme	WPI	695:697	arg1	structure					682:690	the molecular structure	668:690	the molecular structure of WPI	668:697	Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the β-sheet content was decreased and the tryptophan content was increased.					
32449946	0	41	theme	conjugates	113:122	arg1	β-carotene					87:96	β-carotene	87:96	β-carotene of WPI-dextran conjugates	87:122	Effect of reaction temperature on the protein structure and the ability to encapsulate β-carotene of WPI-dextran conjugates.					
32449946	7	42	theme	glycosylation	1289:1301	arg1	reaction					1303:1310	the glycosylation reaction	1285:1310	the glycosylation reaction	1285:1310	Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate β-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation.					
32449946	2	43	theme	WPI-D	554:558	arg1	formation					541:549	the formation	537:549	the formation of WPI-D	537:558	The results of the extent of glycation (EG) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed the formation of WPI-D.					
32449946	1	44	dep	90 °C	326:330	arg1	to					323:324	to	323:324	to	323:324	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	1	45	theme	molecular	340:348	arg1	structure					350:358	molecular structure	340:358	molecular structure	340:358	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	3	46	dep	Fourier	586:592	arg1	transform					594:602	transform	594:602	transform infrared spectrum	594:620	Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the β-sheet content was decreased and the tryptophan content was increased.					
32449946	1	47	theme	molecular	195:203	arg1	structure					205:213	molecular structure	195:213	molecular structure	195:213	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	7	48	theme	glycosylation	1517:1529	arg1	temperature					1502:1512	the reaction temperature	1489:1512	the reaction temperature of glycosylation	1489:1529	Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate β-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation.					
32449946	5	49	theme	protein	1080:1086	arg1	unfolding					1088:1096	protein unfolding	1080:1096	protein unfolding	1080:1096	When the reaction temperature was 70 and 80 °C, the EG and the ability to encapsulate β-carotene of WPI-D were better (P < 0.05), which was related to protein unfolding.					
32449946	7	50	theme	WPI-D	1453:1457	arg1	β-carotene					1439:1448	β-carotene	1439:1448	β-carotene of WPI-D	1439:1457	Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate β-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation.					
32449946	8	51	dep	APPLICATION	1542:1552	arg1	change					1586:1591	change	1586:1591	can change the molecular structure and functional properties of Whey protein isolate	1582:1665	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	7	52	dep	structure	1337:1345	arg1	the					1323:1325	the	1323:1325	the	1323:1325	Therefore, the glycosylation reaction can change the molecular structure and functional properties of WPI; the emulsifying properties and the ability to encapsulate β-carotene of WPI-D can be changed by controlling the reaction temperature of glycosylation.					
32449946	0	53	theme	protein	38:44	arg1	structure					46:54	the protein structure	34:54	the protein structure	34:54	Effect of reaction temperature on the protein structure and the ability to encapsulate β-carotene of WPI-dextran conjugates.					
32449946	3	54	theme	glycosylation-the	717:733	arg1	content					743:749	glycosylation-the β-sheet content	717:749	glycosylation-the β-sheet content	717:749	Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the β-sheet content was decreased and the tryptophan content was increased.					
32449946	6	55	theme	WPI	1146:1148	arg1	molecules					1150:1158	the WPI molecules	1142:1158	the WPI molecules	1142:1158	However, due to the polymerization between the WPI molecules, the emulsion activity index of WPI-D and the ability to encapsulate β-carotene were lowered at 90 °C (P < 0.05).					
32449946	1	56	dep	structure	205:213	arg1	the					191:193	the	191:193	the	191:193	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	3	57	theme	β-sheet	735:741	arg1	content					743:749	glycosylation-the β-sheet content	717:749	glycosylation-the β-sheet content	717:749	Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the β-sheet content was decreased and the tryptophan content was increased.					
32449946	2	58	theme	electrophoresis	511:525	arg1	extent					440:445	the extent	436:445	the extent of glycation (EG) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis	436:525	The results of the extent of glycation (EG) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed the formation of WPI-D.					
32449946	1	59	theme	functional	364:373	arg1	properties					375:384	functional properties	364:384	functional properties	364:384	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	8	60	theme	PRACTICAL	1532:1540	arg1	APPLICATION					1542:1552	PRACTICAL APPLICATION	1532:1552	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.	1532:1834	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	2	61	theme	gel	507:509	arg1	electrophoresis					511:525	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	469:525	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	469:525	The results of the extent of glycation (EG) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed the formation of WPI-D.					
32449946	8	62	dep	structure	1607:1615	arg1	the					1593:1595	the	1593:1595	the	1593:1595	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	1	63	dep	structure	350:358	arg1	the					336:338	the	336:338	the	336:338	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	3	64	theme	Circular	561:568	arg1	dichroism					570:578	Circular dichroism	561:578	Circular dichroism (CD)	561:583	Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the β-sheet content was decreased and the tryptophan content was increased.					
32449946	3	64	theme	Circular	561:568	arg1	CD					581:582	CD	581:582	CD	581:582	Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the β-sheet content was decreased and the tryptophan content was increased.					
32449946	8	65	theme	molecular	1597:1605	arg1	structure					1607:1615	molecular structure	1597:1615	molecular structure	1597:1615	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	1	66	theme	functional	219:228	arg1	properties					230:239	functional properties	219:239	functional properties	219:239	In this paper, we studied the effect of glycosylation reaction on the molecular structure and functional properties of whey protein isolate (WPI), and studied the effect of reaction temperature (50 to 90 °C) on the molecular structure and functional properties of WPI-dextran conjugates (WPI-D).					
32449946	2	67	theme	extent	440:445	arg1	results					425:431	The results	421:431	The results of the extent of glycation (EG) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis	421:525	The results of the extent of glycation (EG) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis confirmed the formation of WPI-D.					
32449946	8	68	theme	glycosylation	1821:1833	arg1	temperature					1806:1816	the reaction temperature	1793:1816	the reaction temperature of glycosylation	1793:1833	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	5	69	dep	were	1035:1038	arg1	P < 0.05					1048:1055	P < 0.05	1048:1055	P < 0.05	1048:1055	When the reaction temperature was 70 and 80 °C, the EG and the ability to encapsulate β-carotene of WPI-D were better (P < 0.05), which was related to protein unfolding.					
32449946	8	70	theme	conjugates	1752:1761	arg1	β-carotene					1726:1735	β-carotene	1726:1735	β-carotene of WPI-dextran conjugates	1726:1761	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	8	71	theme	functional	1621:1630	arg1	properties					1632:1641	functional properties	1621:1641	functional properties	1621:1641	PRACTICAL APPLICATION: The glycosylation reaction can change the molecular structure and functional properties of Whey protein isolate; the emulsifying properties and the ability to encapsulate β-carotene of WPI-dextran conjugates can be changed by controlling the reaction temperature of glycosylation.					
32449946	3	72	dep	transform	594:602	arg1	infrared					604:611	infrared	604:611	transform infrared spectrum	594:620	Circular dichroism (CD), Fourier transform infrared spectrum, and fluorescence spectroscopy indicated that the molecular structure of WPI was changed after glycosylation-the β-sheet content was decreased and the tryptophan content was increased.					
32088200	3	0	theme	Polysialic	332:341	arg1	PSA					349:351	PSA	349:351	PSA	349:351	Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics.					
32088200	3	0	theme	Polysialic	332:341	arg1	conjugate					398:406	a biodegradable and non-immunogenic conjugate	362:406	a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics	362:461	Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics.					
32088200	3	0	theme	Polysialic	332:341	arg1	acid					343:346	Polysialic acid	332:346	Polysialic acid (PSA)	332:352	Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics.					
32088200	8	1	theme	glycan	1169:1174	arg1	backbone					1176:1183	the glycan backbone	1165:1183	the glycan backbone	1165:1183	This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection.					
32088200	5	2	theme	mass	650:653	arg1	spectrometry					655:666	mass spectrometry	650:666	mass spectrometry (MS)	650:671	However, PSA is difficult to analyse by mass spectrometry (MS) due to its high negative charge and size.					
32088200	5	2	theme	mass	650:653	arg1	MS					669:670	MS	669:670	MS	669:670	However, PSA is difficult to analyse by mass spectrometry (MS) due to its high negative charge and size.					
32088200	10	3	from	study	1490:1494	arg1	settings					1558:1565	biological and therapeutic settings	1531:1565	biological and therapeutic settings	1531:1565	This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.					
32088200	10	4	theme	glycoprotein	1499:1510	arg1	polysialylation					1512:1526	glycoprotein polysialylation	1499:1526	glycoprotein polysialylation	1499:1526	This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.					
32088200	4	5	theme	protein	592:598	arg1	function					600:607	protein function	592:607	protein function	592:607	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	8	6	theme	acid	1101:1104	arg1	hydrolysis					1106:1115	mild acid hydrolysis	1096:1115	mild acid hydrolysis	1096:1115	This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection.					
32088200	6	7	theme	occupancy	892:900	arg1	questions					858:866	the key questions	850:866	the key questions of site specificity and occupancy	850:900	Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy.					
32088200	10	8	theme	polysialylation	1512:1526	arg1	study					1490:1494	the study	1486:1494	the study of glycoprotein polysialylation in biological and therapeutic settings	1486:1565	This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.					
32088200	6	9	theme	monosaccharide	803:816	arg1	composition					818:828	monosaccharide composition	803:828	monosaccharide composition	803:828	Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy.					
32088200	8	10	theme	site-specific	1056:1068	arg1	modification					1074:1085	site-specific PSA modification	1056:1085	site-specific PSA modification	1056:1085	This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection.					
32088200	9	11	theme	PSA-modified	1275:1286	arg1	glycopeptides					1288:1300	PSA-modified glycopeptides	1275:1300	PSA-modified glycopeptides	1275:1300	PSA-modified glycopeptides are not detectable by LC-ESI-MS/MS, but become detectable after desialylation, allowing measurement of site-specific PSA occupancy.					
32088200	10	12	theme	analytical	1462:1471	arg1	workflow					1473:1480	an efficient analytical workflow	1449:1480	an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings	1449:1565	This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.					
32088200	10	12	theme	analytical	1462:1471	arg1	method					1439:1444	This method	1434:1444	This method	1434:1444	This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.					
32088200	8	13	theme	PSA	1070:1072	arg1	modification					1074:1085	site-specific PSA modification	1056:1085	site-specific PSA modification	1056:1085	This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection.					
32088200	7	14	theme	high-throughput	924:938	arg1	method					969:974	a high-throughput LC-ESI-MS/MS glycoproteomics method	922:974	a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins	922:1032	Here, we developed a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins.					
32088200	10	15	gly	glycoprotein	1499:1510	arg1	glycoprotein					1499:1510	glycoprotein polysialylation	1499:1526	glycoprotein polysialylation	1499:1526	This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.					
32088200	7	16	theme	site-specific	987:999	arg1	polysialylation					1001:1015	site-specific polysialylation	987:1015	site-specific polysialylation of glycoproteins	987:1032	Here, we developed a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins.					
32088200	6	17	theme	specificity	876:886	arg1	questions					858:866	the key questions	850:866	the key questions of site specificity and occupancy	850:900	Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy.					
32088200	1	18	theme	enzymatic	84:92	arg1	addition					94:101	the enzymatic addition	80:101	the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans	80:191	Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans.					
32088200	1	18	theme	enzymatic	84:92	arg1	Polysialylation					61:75	Polysialylation	61:75	Polysialylation	61:75	Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans.					
32088200	6	19	theme	polymerization	784:797	arg1	degree					774:779	its degree	770:779	its degree of polymerization and monosaccharide composition	770:828	Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy.					
32088200	2	20	theme	tissue	299:304	arg1	regeneration					306:317	neural tissue regeneration	292:317	neural tissue regeneration	292:317	Polysialylation plays an important role in development, and is involved in neurological diseases, neural tissue regeneration, and cancer.					
32088200	4	21	theme	function	600:607	arg1	sites					537:541	the specific sites	524:541	the specific sites of polysialylation	524:560	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	4	21	theme	function	600:607	arg1	polysialylation					546:560	polysialylation	546:560	polysialylation	546:560	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	4	21	theme	function	600:607	arg1	determinants					576:587	important determinants	566:587	important determinants of protein function	566:607	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	6	22	theme	site	871:874	arg1	specificity					876:886	site specificity	871:886	site specificity	871:886	Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy.					
32088200	7	23	theme	LC-ESI-MS/MS	940:951	arg1	method					969:974	a high-throughput LC-ESI-MS/MS glycoproteomics method	922:974	a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins	922:1032	Here, we developed a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins.					
32088200	2	24	theme	neural	292:297	arg1	regeneration					306:317	neural tissue regeneration	292:317	neural tissue regeneration	292:317	Polysialylation plays an important role in development, and is involved in neurological diseases, neural tissue regeneration, and cancer.					
32088200	1	25	theme	glycans	185:191	arg1	termini					174:180	the non-reducing termini	157:180	the non-reducing termini of glycans	157:191	Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans.					
32088200	8	26	theme	glycopeptide	1251:1262	arg1	detection					1264:1272	LC-ESI-MS/MS glycopeptide detection	1238:1272	LC-ESI-MS/MS glycopeptide detection	1238:1272	This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection.					
32088200	10	27	theme	therapeutic	1546:1556	arg1	settings					1558:1565	biological and therapeutic settings	1531:1565	biological and therapeutic settings	1531:1565	This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.					
32088200	3	28	theme	non-immunogenic	382:396	arg1	conjugate					398:406	a biodegradable and non-immunogenic conjugate	362:406	a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics	362:461	Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics.					
32088200	3	28	theme	non-immunogenic	382:396	arg1	acid					343:346	Polysialic acid	332:346	Polysialic acid (PSA)	332:352	Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics.					
32088200	9	29	theme	PSA	1419:1421	arg1	occupancy					1423:1431	site-specific PSA occupancy	1405:1431	site-specific PSA occupancy	1405:1431	PSA-modified glycopeptides are not detectable by LC-ESI-MS/MS, but become detectable after desialylation, allowing measurement of site-specific PSA occupancy.					
32088200	8	30	theme	mild	1096:1099	arg1	hydrolysis					1106:1115	mild acid hydrolysis	1096:1115	mild acid hydrolysis	1096:1115	This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection.					
32088200	0	31	theme	Glycoproteomic	0:13	arg1	measurement					15:25	Glycoproteomic measurement	0:25	Glycoproteomic measurement of site-specific polysialylation	0:58	Glycoproteomic measurement of site-specific polysialylation.					
32088200	7	32	gly	polysialylation	1001:1015	arg1	glycoproteins					1020:1032	glycoproteins	1020:1032	glycoproteins	1020:1032	Here, we developed a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins.					
32088200	10	33	theme	biological	1531:1540	arg1	settings					1558:1565	biological and therapeutic settings	1531:1565	biological and therapeutic settings	1531:1565	This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.					
32088200	8	34	gly	glycopeptide	1251:1262	arg2	glycopeptide					1251:1262	LC-ESI-MS/MS glycopeptide detection	1238:1272	LC-ESI-MS/MS glycopeptide detection	1238:1272	This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection.					
32088200	9	35	gly	glycopeptides	1288:1300	arg2	glycopeptides					1288:1300	PSA-modified glycopeptides	1275:1300	PSA-modified glycopeptides	1275:1300	PSA-modified glycopeptides are not detectable by LC-ESI-MS/MS, but become detectable after desialylation, allowing measurement of site-specific PSA occupancy.					
32088200	3	36	theme	biodegradable	364:376	arg1	conjugate					398:406	a biodegradable and non-immunogenic conjugate	362:406	a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics	362:461	Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics.					
32088200	3	36	theme	biodegradable	364:376	arg1	acid					343:346	Polysialic acid	332:346	Polysialic acid (PSA)	332:352	Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics.					
32088200	4	37	theme	polysialylation	546:560	arg1	sites					537:541	the specific sites	524:541	the specific sites of polysialylation	524:560	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	4	37	theme	polysialylation	546:560	arg1	polysialylation					546:560	polysialylation	546:560	polysialylation	546:560	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	4	37	theme	polysialylation	546:560	arg1	determinants					576:587	important determinants	566:587	important determinants of protein function	566:607	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	5	38	theme	high	684:687	arg1	charge					698:703	its high negative charge	680:703	its high negative charge	680:703	However, PSA is difficult to analyse by mass spectrometry (MS) due to its high negative charge and size.					
32088200	6	39	theme	PSA	758:760	arg1	analysis					746:753	analysis	746:753	analysis of PSA	746:760	Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy.					
32088200	7	40	theme	glycoproteomics	953:967	arg1	method					969:974	a high-throughput LC-ESI-MS/MS glycoproteomics method	922:974	a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins	922:1032	Here, we developed a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins.					
32088200	9	41	theme	site-specific	1405:1417	arg1	occupancy					1423:1431	site-specific PSA occupancy	1405:1431	site-specific PSA occupancy	1405:1431	PSA-modified glycopeptides are not detectable by LC-ESI-MS/MS, but become detectable after desialylation, allowing measurement of site-specific PSA occupancy.					
32088200	5	42	theme	negative	689:696	arg1	charge					698:703	its high negative charge	680:703	its high negative charge	680:703	However, PSA is difficult to analyse by mass spectrometry (MS) due to its high negative charge and size.					
32088200	6	43	theme	key	854:856	arg1	questions					858:866	the key questions	850:866	the key questions of site specificity and occupancy	850:900	Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy.					
32088200	1	44	theme	charged	126:132	arg1	acid					141:144	a highly negatively charged sialic acid	106:144	a highly negatively charged sialic acid polymer	106:152	Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans.					
32088200	8	45	theme	LC-ESI-MS/MS	1238:1249	arg1	detection					1264:1272	LC-ESI-MS/MS glycopeptide detection	1238:1272	LC-ESI-MS/MS glycopeptide detection	1238:1272	This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection.					
32088200	4	46	theme	important	566:574	arg1	sites					537:541	the specific sites	524:541	the specific sites of polysialylation	524:560	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	4	46	theme	important	566:574	arg1	polysialylation					546:560	polysialylation	546:560	polysialylation	546:560	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	4	46	theme	important	566:574	arg1	determinants					576:587	important determinants	566:587	important determinants of protein function	566:607	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	4	47	theme	PSA	464:466	arg1	chains					468:473	PSA chains	464:473	PSA chains	464:473	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	0	48	theme	polysialylation	44:58	arg1	measurement					15:25	Glycoproteomic measurement	0:25	Glycoproteomic measurement of site-specific polysialylation	0:58	Glycoproteomic measurement of site-specific polysialylation.					
32088200	4	49	theme	specific	528:535	arg1	sites					537:541	the specific sites	524:541	the specific sites of polysialylation	524:560	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	4	49	theme	specific	528:535	arg1	polysialylation					546:560	polysialylation	546:560	polysialylation	546:560	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	4	49	theme	specific	528:535	arg1	determinants					576:587	important determinants	566:587	important determinants of protein function	566:607	PSA chains vary in length, composition, and linkages, while the specific sites of polysialylation are important determinants of protein function.					
32088200	0	50	theme	site-specific	30:42	arg1	polysialylation					44:58	site-specific polysialylation	30:58	site-specific polysialylation	30:58	Glycoproteomic measurement of site-specific polysialylation.					
32088200	8	51	theme	protease	1207:1214	arg1	digestion					1216:1224	protease digestion	1207:1224	protease digestion followed by LC-ESI-MS/MS glycopeptide detection	1207:1272	This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection.					
32088200	9	52	theme	occupancy	1423:1431	arg1	measurement					1390:1400	measurement	1390:1400	measurement of site-specific PSA occupancy	1390:1431	PSA-modified glycopeptides are not detectable by LC-ESI-MS/MS, but become detectable after desialylation, allowing measurement of site-specific PSA occupancy.					
32088200	10	53	theme	efficient	1452:1460	arg1	workflow					1473:1480	an efficient analytical workflow	1449:1480	an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings	1449:1565	This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.					
32088200	10	53	theme	efficient	1452:1460	arg1	method					1439:1444	This method	1434:1444	This method	1434:1444	This method is an efficient analytical workflow for the study of glycoprotein polysialylation in biological and therapeutic settings.					
32088200	1	54	theme	sialic	134:139	arg1	acid					141:144	a highly negatively charged sialic acid	106:144	a highly negatively charged sialic acid polymer	106:152	Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans.					
32088200	3	55	theme	therapeutic	411:421	arg1	drugs					423:427	therapeutic drugs	411:427	therapeutic drugs	411:427	Polysialic acid (PSA) is also a biodegradable and non-immunogenic conjugate to therapeutic drugs to improve their pharmacokinetics.					
32088200	7	56	gly	glycoproteins	1020:1032	arg1	glycoproteins					1020:1032	glycoproteins	1020:1032	glycoproteins	1020:1032	Here, we developed a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins.					
32088200	1	57	theme	acid	141:144	arg1	polymer					146:152	a highly negatively charged sialic acid polymer	106:152	a highly negatively charged sialic acid polymer	106:152	Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans.					
32088200	7	58	theme	glycoproteins	1020:1032	arg1	polysialylation					1001:1015	site-specific polysialylation	987:1015	site-specific polysialylation of glycoproteins	987:1032	Here, we developed a high-throughput LC-ESI-MS/MS glycoproteomics method to measure site-specific polysialylation of glycoproteins.					
32088200	1	59	theme	polymer	146:152	arg1	addition					94:101	the enzymatic addition	80:101	the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans	80:191	Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans.					
32088200	1	59	theme	polymer	146:152	arg1	Polysialylation					61:75	Polysialylation	61:75	Polysialylation	61:75	Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans.					
32088200	8	60	theme	sialic	1138:1143	arg1	acids					1145:1149	sialic acids	1138:1149	sialic acids	1138:1149	This method measures site-specific PSA modification by using mild acid hydrolysis to eliminate PSA and sialic acids while leaving the glycan backbone intact, together with protease digestion followed by LC-ESI-MS/MS glycopeptide detection.					
32088200	2	61	theme	important	219:227	arg1	role					229:232	an important role	216:232	an important role	216:232	Polysialylation plays an important role in development, and is involved in neurological diseases, neural tissue regeneration, and cancer.					
32088200	2	62	theme	neurological	269:280	arg1	diseases					282:289	neurological diseases	269:289	neurological diseases	269:289	Polysialylation plays an important role in development, and is involved in neurological diseases, neural tissue regeneration, and cancer.					
32088200	6	63	theme	analytical	720:729	arg1	approaches					731:740	Most analytical approaches	715:740	Most analytical approaches for analysis of PSA	715:760	Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy.					
32088200	6	64	theme	composition	818:828	arg1	degree					774:779	its degree	770:779	its degree of polymerization and monosaccharide composition	770:828	Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy.					
32088200	6	65	theme	Most	715:718	arg1	approaches					731:740	Most analytical approaches	715:740	Most analytical approaches for analysis of PSA	715:760	Most analytical approaches for analysis of PSA measure its degree of polymerization and monosaccharide composition, but do not address the key questions of site specificity and occupancy.					
32088200	1	66	theme	non-reducing	161:172	arg1	termini					174:180	the non-reducing termini	157:180	the non-reducing termini of glycans	157:191	Polysialylation is the enzymatic addition of a highly negatively charged sialic acid polymer to the non-reducing termini of glycans.					
32088200	2	67	gly	Polysialylation	194:208	arg1	regeneration					306:317	neural tissue regeneration	292:317	neural tissue regeneration	292:317	Polysialylation plays an important role in development, and is involved in neurological diseases, neural tissue regeneration, and cancer.					
32088200	2	67	gly	Polysialylation	194:208	arg1	cancer					324:329	cancer	324:329	cancer	324:329	Polysialylation plays an important role in development, and is involved in neurological diseases, neural tissue regeneration, and cancer.					
32088200	2	67	gly	Polysialylation	194:208	arg1	diseases					282:289	neurological diseases	269:289	neurological diseases	269:289	Polysialylation plays an important role in development, and is involved in neurological diseases, neural tissue regeneration, and cancer.					
33142561	5	0	theme	chain	1098:1102	arg1	decasaccharide					1114:1127	decasaccharide	1114:1127	decasaccharide	1114:1127	It is possible to replace GlcUAβ-1-3GlcNAc of the internal disaccharide units with GlcUAβ-1-3N-acetylgalactosamine (GalNAc), if the chain length is decasaccharide or larger.					
33142561	5	0	theme	chain	1098:1102	arg1	length					1104:1109	the chain length	1094:1109	the chain length	1094:1109	It is possible to replace GlcUAβ-1-3GlcNAc of the internal disaccharide units with GlcUAβ-1-3N-acetylgalactosamine (GalNAc), if the chain length is decasaccharide or larger.					
33142561	3	1	theme	hybrid	473:478	arg1	oligosaccharides					480:495	hybrid oligosaccharides	473:495	hybrid oligosaccharides	473:495	We prepared hybrid oligosaccharides from hyaluronan and chondroitin, with or without modification of the reducing or non-reducing terminus, as tools to determine the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method.					
33142561	3	1	theme	hybrid	473:478	arg1	tools					604:608	tools	604:608	tools	604:608	We prepared hybrid oligosaccharides from hyaluronan and chondroitin, with or without modification of the reducing or non-reducing terminus, as tools to determine the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method.					
33142561	0	2	theme	glycotechnological	96:113	arg1	approach					115:122	glycotechnological approach	96:122	glycotechnological approach	96:122	Essential hyaluronan structure for binding with hyaluronan-binding protein (HABP) determined by glycotechnological approach.					
33142561	5	3	with	GlcUAβ-1-3GlcNAc	992:1007	arg1	GalNAc					1082:1087	GalNAc	1082:1087	GalNAc	1082:1087	It is possible to replace GlcUAβ-1-3GlcNAc of the internal disaccharide units with GlcUAβ-1-3N-acetylgalactosamine (GalNAc), if the chain length is decasaccharide or larger.					
33142561	5	3	with	GlcUAβ-1-3GlcNAc	992:1007	arg1	GlcUAβ-1-3N-acetylgalactosamine					1049:1079	GlcUAβ-1-3N-acetylgalactosamine	1049:1079	GlcUAβ-1-3N-acetylgalactosamine (GalNAc)	1049:1088	It is possible to replace GlcUAβ-1-3GlcNAc of the internal disaccharide units with GlcUAβ-1-3N-acetylgalactosamine (GalNAc), if the chain length is decasaccharide or larger.					
33142561	3	4	theme	competitive	690:700	arg1	method					713:718	a competitive ELISA-like method	688:718	a competitive ELISA-like method	688:718	We prepared hybrid oligosaccharides from hyaluronan and chondroitin, with or without modification of the reducing or non-reducing terminus, as tools to determine the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method.					
33142561	4	5	theme	terminal	738:745	arg1	N-acetylglucosamine					815:833	N-acetylglucosamine	815:833	N-acetylglucosamine (GlcNAc)	815:842	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	5	theme	terminal	738:745	arg1	structure					747:755	The non-reducing terminal structure	721:755	The non-reducing terminal structure was critical	721:768	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	5	theme	terminal	738:745	arg1	essential					871:879	essential	871:879	essential	871:879	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	5	theme	terminal	738:745	arg1	acid					798:801	the glucuronic acid	783:801	the glucuronic acid (GlcUA)	783:809	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	3	6	theme	ELISA-like	702:711	arg1	method					713:718	a competitive ELISA-like method	688:718	a competitive ELISA-like method	688:718	We prepared hybrid oligosaccharides from hyaluronan and chondroitin, with or without modification of the reducing or non-reducing terminus, as tools to determine the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method.					
33142561	2	7	theme	present	369:375	arg1	study					377:381	the present study	365:381	the present study	365:381	The aim of the present study was to investigate the important structure of hyaluronan for binding to HABP.					
33142561	0	8	theme	hyaluronan-binding	48:65	arg1	HABP					76:79	HABP	76:79	HABP	76:79	Essential hyaluronan structure for binding with hyaluronan-binding protein (HABP) determined by glycotechnological approach.					
33142561	0	8	theme	hyaluronan-binding	48:65	arg1	protein					67:73	hyaluronan-binding protein	48:73	hyaluronan-binding protein (HABP)	48:80	Essential hyaluronan structure for binding with hyaluronan-binding protein (HABP) determined by glycotechnological approach.					
33142561	0	9	theme	hyaluronan	10:19	arg1	structure					21:29	Essential hyaluronan structure	0:29	Essential hyaluronan structure for binding with hyaluronan-binding protein (HABP)	0:80	Essential hyaluronan structure for binding with hyaluronan-binding protein (HABP) determined by glycotechnological approach.					
33142561	0	10	theme	Essential	0:8	arg1	structure					21:29	Essential hyaluronan structure	0:29	Essential hyaluronan structure for binding with hyaluronan-binding protein (HABP)	0:80	Essential hyaluronan structure for binding with hyaluronan-binding protein (HABP) determined by glycotechnological approach.					
33142561	2	11	theme	important	406:414	arg1	structure					416:424	the important structure	402:424	the important structure of hyaluronan for binding to HABP	402:458	The aim of the present study was to investigate the important structure of hyaluronan for binding to HABP.					
33142561	4	12	theme	HABP	894:897	arg1	activity					907:914	complete HABP binding activity	885:914	complete HABP binding activity	885:914	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	13	theme	non-reducing	725:736	arg1	N-acetylglucosamine					815:833	N-acetylglucosamine	815:833	N-acetylglucosamine (GlcNAc)	815:842	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	13	theme	non-reducing	725:736	arg1	structure					747:755	The non-reducing terminal structure	721:755	The non-reducing terminal structure was critical	721:768	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	13	theme	non-reducing	725:736	arg1	essential					871:879	essential	871:879	essential	871:879	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	13	theme	non-reducing	725:736	arg1	acid					798:801	the glucuronic acid	783:801	the glucuronic acid (GlcUA)	783:809	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	1	14	theme	hyaluronan	269:278	arg1	protein					240:246	hyaluronan binding protein	221:246	just hyaluronan binding protein (HABP)	216:253	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	1	14	theme	hyaluronan	269:278	arg1	structure					293:301	the hyaluronan carbohydrate structure	265:301	the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail	265:351	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	4	15	theme	complete	885:892	arg1	activity					907:914	complete HABP binding activity	885:914	complete HABP binding activity	885:914	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	5	16	theme	units	1038:1042	arg1	GlcUAβ-1-3GlcNAc					992:1007	GlcUAβ-1-3GlcNAc	992:1007	GlcUAβ-1-3GlcNAc of the internal disaccharide units with GlcUAβ-1-3N-acetylgalactosamine (GalNAc)	992:1088	It is possible to replace GlcUAβ-1-3GlcNAc of the internal disaccharide units with GlcUAβ-1-3N-acetylgalactosamine (GalNAc), if the chain length is decasaccharide or larger.					
33142561	1	17	theme	carbohydrate	280:291	arg1	protein					240:246	hyaluronan binding protein	221:246	just hyaluronan binding protein (HABP)	216:253	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	1	17	theme	carbohydrate	280:291	arg1	structure					293:301	the hyaluronan carbohydrate structure	265:301	the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail	265:351	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	3	18	theme	preferred	627:635	arg1	structure					637:645	the preferred structure	623:645	the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method	623:718	We prepared hybrid oligosaccharides from hyaluronan and chondroitin, with or without modification of the reducing or non-reducing terminus, as tools to determine the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method.					
33142561	4	19	theme	binding	899:905	arg1	activity					907:914	complete HABP binding activity	885:914	complete HABP binding activity	885:914	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	3	20	theme	non-reducing	578:589	arg1	terminus					591:598	the reducing or non-reducing terminus	562:598	the reducing or non-reducing terminus	562:598	We prepared hybrid oligosaccharides from hyaluronan and chondroitin, with or without modification of the reducing or non-reducing terminus, as tools to determine the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method.					
33142561	2	21	theme	study	377:381	arg1	aim					358:360	The aim	354:360	The aim of the present study	354:381	The aim of the present study was to investigate the important structure of hyaluronan for binding to HABP.					
33142561	0	22	with	structure	21:29	arg1	HABP					76:79	HABP	76:79	HABP	76:79	Essential hyaluronan structure for binding with hyaluronan-binding protein (HABP) determined by glycotechnological approach.					
33142561	0	22	with	structure	21:29	arg1	protein					67:73	hyaluronan-binding protein	48:73	hyaluronan-binding protein (HABP)	48:80	Essential hyaluronan structure for binding with hyaluronan-binding protein (HABP) determined by glycotechnological approach.					
33142561	2	23	theme	hyaluronan	429:438	arg1	structure					416:424	the important structure	402:424	the important structure of hyaluronan for binding to HABP	402:458	The aim of the present study was to investigate the important structure of hyaluronan for binding to HABP.					
33142561	4	24	theme	hyaluronan-unit	851:865	arg1	N-acetylglucosamine					815:833	N-acetylglucosamine	815:833	N-acetylglucosamine (GlcNAc)	815:842	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	24	theme	hyaluronan-unit	851:865	arg1	structure					747:755	The non-reducing terminal structure	721:755	The non-reducing terminal structure was critical	721:768	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	24	theme	hyaluronan-unit	851:865	arg1	essential					871:879	essential	871:879	essential	871:879	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	24	theme	hyaluronan-unit	851:865	arg1	acid					798:801	the glucuronic acid	783:801	the glucuronic acid (GlcUA)	783:809	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	3	25	theme	hyaluronan	650:659	arg1	structure					637:645	the preferred structure	623:645	the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method	623:718	We prepared hybrid oligosaccharides from hyaluronan and chondroitin, with or without modification of the reducing or non-reducing terminus, as tools to determine the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method.					
33142561	3	26	theme	reducing	566:573	arg1	terminus					591:598	the reducing or non-reducing terminus	562:598	the reducing or non-reducing terminus	562:598	We prepared hybrid oligosaccharides from hyaluronan and chondroitin, with or without modification of the reducing or non-reducing terminus, as tools to determine the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method.					
33142561	4	27	theme	glucuronic	787:796	arg1	structure					747:755	The non-reducing terminal structure	721:755	The non-reducing terminal structure was critical	721:768	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	27	theme	glucuronic	787:796	arg1	acid					798:801	the glucuronic acid	783:801	the glucuronic acid (GlcUA)	783:809	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	4	27	theme	glucuronic	787:796	arg1	GlcUA					804:808	GlcUA	804:808	GlcUA	804:808	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	1	28	theme	hyaluronan	221:230	arg1	protein					240:246	hyaluronan binding protein	221:246	just hyaluronan binding protein (HABP)	216:253	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	1	28	theme	hyaluronan	221:230	arg1	domain					176:181	aggrecan globular domain 1	158:183	aggrecan globular domain 1	158:183	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	1	28	theme	hyaluronan	221:230	arg1	structure					293:301	the hyaluronan carbohydrate structure	265:301	the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail	265:351	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	1	28	theme	hyaluronan	221:230	arg1	HABP					249:252	HABP	249:252	HABP	249:252	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	5	29	theme	internal	1016:1023	arg1	units					1038:1042	the internal disaccharide units	1012:1042	the internal disaccharide units	1012:1042	It is possible to replace GlcUAβ-1-3GlcNAc of the internal disaccharide units with GlcUAβ-1-3N-acetylgalactosamine (GalNAc), if the chain length is decasaccharide or larger.					
33142561	1	30	theme	binding	232:238	arg1	protein					240:246	hyaluronan binding protein	221:246	just hyaluronan binding protein (HABP)	216:253	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	1	30	theme	binding	232:238	arg1	domain					176:181	aggrecan globular domain 1	158:183	aggrecan globular domain 1	158:183	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	1	30	theme	binding	232:238	arg1	structure					293:301	the hyaluronan carbohydrate structure	265:301	the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail	265:351	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	1	30	theme	binding	232:238	arg1	HABP					249:252	HABP	249:252	HABP	249:252	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	4	31	theme	binding	934:940	arg1	activity					942:949	any HABP binding activity	925:949	any HABP binding activity	925:949	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	5	32	theme	disaccharide	1025:1036	arg1	units					1038:1042	the internal disaccharide units	1012:1042	the internal disaccharide units	1012:1042	It is possible to replace GlcUAβ-1-3GlcNAc of the internal disaccharide units with GlcUAβ-1-3N-acetylgalactosamine (GalNAc), if the chain length is decasaccharide or larger.					
33142561	3	33	theme	terminus	591:598	arg1	modification					546:557	modification	546:557	modification of the reducing or non-reducing terminus	546:598	We prepared hybrid oligosaccharides from hyaluronan and chondroitin, with or without modification of the reducing or non-reducing terminus, as tools to determine the preferred structure of hyaluronan for binding to the HABP by a competitive ELISA-like method.					
33142561	1	34	theme	aggrecan	158:165	arg1	protein					240:246	hyaluronan binding protein	221:246	just hyaluronan binding protein (HABP)	216:253	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	1	34	theme	aggrecan	158:165	arg1	domain					176:181	aggrecan globular domain 1	158:183	aggrecan globular domain 1	158:183	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	4	35	theme	HABP	929:932	arg1	activity					942:949	any HABP binding activity	925:949	any HABP binding activity	925:949	The non-reducing terminal structure was critical, especially, the glucuronic acid (GlcUA) and N-acetylglucosamine (GlcNAc) of the hyaluronan-unit are essential for complete HABP binding activity, and for any HABP binding activity, respectively.					
33142561	1	36	theme	globular	167:174	arg1	protein					240:246	hyaluronan binding protein	221:246	just hyaluronan binding protein (HABP)	216:253	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
33142561	1	36	theme	globular	167:174	arg1	domain					176:181	aggrecan globular domain 1	158:183	aggrecan globular domain 1	158:183	Hyaluronan specifically binds to aggrecan globular domain 1, which is often referred to as just hyaluronan binding protein (HABP), however, the hyaluronan carbohydrate structure recognized by HABP had not been studied in detail.					
32433878	7	0	theme	culture	1805:1811	arg1	profile					1783:1789	the glyco-signature profile	1763:1789	the glyco-signature profile of a neuronal culture after hydrogel treatment	1763:1836	The glycoconjugate biopolymers were used to formulate hydrogels that were chemically and biologically characterized, and the glyco-signature profile of a neuronal culture after hydrogel treatment was analyzed by lectin staining.					
32433878	9	1	from	matrix	2129:2134	arg1	present					2114:2120	present	2114:2120	present	2114:2120	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	1	2	gly	glycosylation	220:232	arg1	ECM					288:290	ECM	288:290	ECM	288:290	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	1	2	gly	glycosylation	220:232	arg1	surface					254:260	the cell surface	245:260	the cell surface	245:260	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	1	2	gly	glycosylation	220:232	arg1	matrix					280:285	extracellular matrix	266:285	extracellular matrix (ECM)	266:291	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	6	3	theme	Gal	1473:1475	arg1	β-Glc					1482:1486	Gal(β1-4)β-Glc	1473:1486	Gal(β1-4)β-Glc	1473:1486	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	6	3	theme	Gal	1473:1475	arg1	lactose					1464:1470	lactose	1464:1470	lactose (Gal(β1-4)β-Glc)	1464:1487	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	2	4	theme	natural	603:609	arg1	components					611:620	natural components	603:620	natural components	603:620	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	9	5	attach	present	2114:2120	arg2	residues					2105:2112	glycan residues	2098:2112	glycan residues present in the matrix	2098:2134	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	9	5	attach	present	2114:2120	arg1	matrix					2129:2134	the matrix	2125:2134	the matrix	2125:2134	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	3	6	theme	cells	733:737	arg1	delivery					721:728	the delivery	717:728	the delivery of cells into the brain	717:752	Collagen hydrogels have shown potential as a vehicle for the delivery of cells into the brain, and their therapeutic effect can be expanded by functionalizing them to address the glycosylation patterns altered with specific brain disorders.					
32433878	6	7	theme	sugar	1539:1543	arg1	structure					1513:1521	the pyranosidic structure	1497:1521	the pyranosidic structure of the reducing sugar	1497:1543	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	1	8	theme	cell	249:252	arg1	surface					254:260	the cell surface	245:260	the cell surface	245:260	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	1	9	theme	nervous	180:186	arg1	system					188:193	the central nervous system	168:193	the central nervous system (CNS)	168:199	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	1	9	theme	nervous	180:186	arg1	CNS					196:198	CNS	196:198	CNS	196:198	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	6	10	theme	collagen	1373:1380	arg1	polymers					1382:1389	collagen polymers	1373:1389	collagen polymers	1373:1389	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	9	11	theme	cell	2200:2203	arg1	systems					2214:2220	cell delivery systems	2200:2220	cell delivery systems in the central nervous system	2200:2250	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	8	12	theme	astrocytic	1920:1929	arg1	proliferation					1931:1943	the astrocytic proliferation	1916:1943	the astrocytic proliferation	1916:1943	The hydrogel conjugated with glucose limited the astrocytic proliferation for up to 2 weeks and promoted the increase in sialylation while decreasing fucosylation by 2-fold.					
32433878	5	13	from	level	1349:1353	arg1	modulation					1303:1312	a differential modulation	1288:1312	a differential modulation of glycan expression at the tissue level	1288:1353	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	5	13	from	level	1349:1353	arg1	expression					1324:1333	glycan expression	1317:1333	glycan expression at the tissue level	1317:1353	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	2	14	theme	biomaterials	569:580	arg1	organization					635:646	physical organization	626:646	physical organization of the ECM	626:657	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	2	14	theme	biomaterials	569:580	arg1	use					562:564	the use	558:564	the use of biomaterials able to recapitulate natural components	558:620	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	9	15	contain	have	2174:2177	arg1	results					2051:2057	These results	2045:2057	These results	2045:2057	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	9	15	contain	have	2174:2177	arg2	potential					2179:2187	potential	2179:2187	potential to enhance cell delivery systems in the central nervous system	2179:2250	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	2	16	used	used	424:427	arg2	strategies					362:371	Regenerative medicine-based strategies	334:371	Regenerative medicine-based strategies using biomaterial platforms	334:399	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	3	17	theme	glycosylation	839:851	arg1	patterns					853:860	the glycosylation patterns	835:860	the glycosylation patterns altered with specific brain disorders	835:898	Collagen hydrogels have shown potential as a vehicle for the delivery of cells into the brain, and their therapeutic effect can be expanded by functionalizing them to address the glycosylation patterns altered with specific brain disorders.					
32433878	2	18	theme	physiological	494:506	arg1	conditions					525:534	the physiological and pathological conditions	490:534	the physiological and pathological conditions	490:534	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	8	19	from	increase	1980:1987	arg1	sialylation					1992:2002	sialylation	1992:2002	sialylation	1992:2002	The hydrogel conjugated with glucose limited the astrocytic proliferation for up to 2 weeks and promoted the increase in sialylation while decreasing fucosylation by 2-fold.					
32433878	5	20	theme	tissue	1342:1347	arg1	level					1349:1353	the tissue level	1338:1353	the tissue level	1338:1353	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	9	21	theme	nervous	2237:2243	arg1	system					2245:2250	the central nervous system	2225:2250	the central nervous system	2225:2250	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	0	22	theme	Neuronal	76:83	arg1	Culture					93:99	a Neuronal Primary Culture	74:99	a Neuronal Primary Culture	74:99	Glycan-Functionalized Collagen Hydrogels Modulate the Glycoenvironment of a Neuronal Primary Culture.					
32433878	1	23	theme	extracellular	266:278	arg1	ECM					288:290	ECM	288:290	ECM	288:290	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	1	23	theme	extracellular	266:278	arg1	matrix					280:285	extracellular matrix	266:285	extracellular matrix (ECM)	266:291	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	9	24	theme	sugar	2148:2152	arg1	expression					2154:2163	cellular sugar expression	2139:2163	cellular sugar expression	2139:2163	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	0	25	theme	Culture	93:99	arg1	Glycoenvironment					54:69	the Glycoenvironment	50:69	the Glycoenvironment of a Neuronal Primary Culture	50:99	Glycan-Functionalized Collagen Hydrogels Modulate the Glycoenvironment of a Neuronal Primary Culture.					
32433878	3	26	theme	specific	875:882	arg1	disorders					890:898	specific brain disorders	875:898	specific brain disorders	875:898	Collagen hydrogels have shown potential as a vehicle for the delivery of cells into the brain, and their therapeutic effect can be expanded by functionalizing them to address the glycosylation patterns altered with specific brain disorders.					
32433878	5	27	theme	glycan	1317:1322	arg1	expression					1324:1333	glycan expression	1317:1333	glycan expression at the tissue level	1317:1353	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	6	28	theme	step	1405:1408	arg1	amination					1420:1428	one step reductive amination	1401:1428	one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker)	1401:1584	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	7	29	used	used	1678:1681	arg2	biopolymers					1661:1671	The glycoconjugate biopolymers	1642:1671	The glycoconjugate biopolymers	1642:1671	The glycoconjugate biopolymers were used to formulate hydrogels that were chemically and biologically characterized, and the glyco-signature profile of a neuronal culture after hydrogel treatment was analyzed by lectin staining.					
32433878	2	30	theme	pathological	512:523	arg1	conditions					525:534	the physiological and pathological conditions	490:534	the physiological and pathological conditions	490:534	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	2	31	theme	ECM	655:657	arg1	organization					635:646	physical organization	626:646	physical organization of the ECM	626:657	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	2	31	theme	ECM	655:657	arg1	use					562:564	the use	558:564	the use of biomaterials able to recapitulate natural components	558:620	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	6	32	theme	α-Glc	1594:1598	arg1	residues					1610:1617	the α-Glc and β-Gal residues	1590:1617	residues	1610:1617	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	7	33	theme	glycoconjugate	1646:1659	arg1	biopolymers					1661:1671	The glycoconjugate biopolymers	1642:1671	The glycoconjugate biopolymers	1642:1671	The glycoconjugate biopolymers were used to formulate hydrogels that were chemically and biologically characterized, and the glyco-signature profile of a neuronal culture after hydrogel treatment was analyzed by lectin staining.					
32433878	8	34	dep	2	1955:1955	arg1	to					1952:1953	to	1952:1953	to	1952:1953	The hydrogel conjugated with glucose limited the astrocytic proliferation for up to 2 weeks and promoted the increase in sialylation while decreasing fucosylation by 2-fold.					
32433878	4	35	theme	collagen-based	990:1003	arg1	hydrogel					1005:1012	a glycan-functionalized tridimensional collagen-based hydrogel	951:1012	a glycan-functionalized tridimensional collagen-based hydrogel that will make contact with and modulate the differentiation of a primary neuronal culture	951:1103	The goal of this study is to develop and optimize a glycan-functionalized tridimensional collagen-based hydrogel that will make contact with and modulate the differentiation of a primary neuronal culture.					
32433878	0	36	theme	Glycan-Functionalized	0:20	arg1	Hydrogels					31:39	Glycan-Functionalized Collagen Hydrogels	0:39	Glycan-Functionalized Collagen Hydrogels	0:39	Glycan-Functionalized Collagen Hydrogels Modulate the Glycoenvironment of a Neuronal Primary Culture.					
32433878	1	37	theme	surface	254:260	arg1	profile					234:240	the glycosylation profile	216:240	the glycosylation profile of the cell surface and extracellular matrix (ECM) components	216:302	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	6	38	with	residues	1610:1617	arg1	β-Glc					1482:1486	Gal(β1-4)β-Glc	1473:1486	Gal(β1-4)β-Glc	1473:1486	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	6	38	with	residues	1610:1617	arg1	maltose					1435:1441	maltose	1435:1441	maltose (Glc(α1-4)α-Glc)	1435:1458	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	6	38	with	residues	1610:1617	arg1	α-Glc					1453:1457	Glc(α1-4)α-Glc	1444:1457	Glc(α1-4)α-Glc	1444:1457	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	6	38	with	residues	1610:1617	arg1	lactose					1464:1470	lactose	1464:1470	lactose (Gal(β1-4)β-Glc)	1464:1487	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	5	39	theme	glyco-signature	1218:1232	arg1	profile					1234:1240	the native glyco-signature profile	1207:1240	the native glyco-signature profile during the process of cell differentiation	1207:1283	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	1	40	theme	central	117:123	arg1	role					125:128	a central role	115:128	a central role	115:128	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	9	41	theme	differential	2072:2083	arg1	influence					2085:2093	the differential influence	2068:2093	the differential influence of glycan residues present in the matrix on cellular sugar expression	2068:2163	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	6	42	theme	α1-4	1448:1451	arg1	maltose					1435:1441	maltose	1435:1441	maltose (Glc(α1-4)α-Glc)	1435:1458	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	6	42	theme	α1-4	1448:1451	arg1	α-Glc					1453:1457	Glc(α1-4)α-Glc	1444:1457	Glc(α1-4)α-Glc	1444:1457	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	5	43	theme	glyco-engineered	1167:1182	arg1	material					1184:1191	a glyco-engineered material	1165:1191	a glyco-engineered material	1165:1191	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	6	44	theme	Glc	1444:1446	arg1	maltose					1435:1441	maltose	1435:1441	maltose (Glc(α1-4)α-Glc)	1435:1458	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	6	44	theme	Glc	1444:1446	arg1	α-Glc					1453:1457	Glc(α1-4)α-Glc	1444:1457	Glc(α1-4)α-Glc	1444:1457	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	9	45	theme	residues	2105:2112	arg1	influence					2085:2093	the differential influence	2068:2093	the differential influence of glycan residues present in the matrix on cellular sugar expression	2068:2163	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	5	46	theme	cell	1264:1267	arg1	differentiation					1269:1283	cell differentiation	1264:1283	cell differentiation	1264:1283	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	2	47	theme	medicine-based	347:360	arg1	strategies					362:371	Regenerative medicine-based strategies	334:371	Regenerative medicine-based strategies using biomaterial platforms	334:399	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	7	48	theme	neuronal	1796:1803	arg1	culture					1805:1811	a neuronal culture	1794:1811	a neuronal culture	1794:1811	The glycoconjugate biopolymers were used to formulate hydrogels that were chemically and biologically characterized, and the glyco-signature profile of a neuronal culture after hydrogel treatment was analyzed by lectin staining.					
32433878	9	49	from	present	2114:2120	arg1	matrix					2129:2134	the matrix	2125:2134	the matrix	2125:2134	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	9	50	from	systems	2214:2220	arg1	system					2245:2250	the central nervous system	2225:2250	the central nervous system	2225:2250	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	4	51	theme	neuronal	1088:1095	arg1	culture					1097:1103	a primary neuronal culture	1078:1103	a primary neuronal culture	1078:1103	The goal of this study is to develop and optimize a glycan-functionalized tridimensional collagen-based hydrogel that will make contact with and modulate the differentiation of a primary neuronal culture.					
32433878	9	52	theme	cellular	2139:2146	arg1	expression					2154:2163	cellular sugar expression	2139:2163	cellular sugar expression	2139:2163	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	7	53	theme	glyco-signature	1767:1781	arg1	profile					1783:1789	the glyco-signature profile	1763:1789	the glyco-signature profile of a neuronal culture after hydrogel treatment	1763:1836	The glycoconjugate biopolymers were used to formulate hydrogels that were chemically and biologically characterized, and the glyco-signature profile of a neuronal culture after hydrogel treatment was analyzed by lectin staining.					
32433878	4	54	theme	primary	1080:1086	arg1	culture					1097:1103	a primary neuronal culture	1078:1103	a primary neuronal culture	1078:1103	The goal of this study is to develop and optimize a glycan-functionalized tridimensional collagen-based hydrogel that will make contact with and modulate the differentiation of a primary neuronal culture.					
32433878	6	55	theme	reducing	1530:1537	arg1	sugar					1539:1543	the reducing sugar	1526:1543	the reducing sugar	1526:1543	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	1	56	theme	central	172:178	arg1	system					188:193	the central nervous system	168:193	the central nervous system (CNS)	168:199	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	1	56	theme	central	172:178	arg1	CNS					196:198	CNS	196:198	CNS	196:198	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	1	57	from	evident	308:314	arg1	disorders					323:331	CNS disorders	319:331	CNS disorders	319:331	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	5	58	from	modulation	1303:1312	arg1	level					1349:1353	the tissue level	1338:1353	the tissue level	1338:1353	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	1	59	theme	system	188:193	arg1	homeostasis					153:163	homeostasis	153:163	homeostasis	153:163	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	1	59	theme	system	188:193	arg1	development					137:147	development	137:147	development	137:147	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	2	60	theme	physical	626:633	arg1	organization					635:646	physical organization	626:646	physical organization of the ECM	626:657	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	6	61	theme	pyranosidic	1501:1511	arg1	structure					1513:1521	the pyranosidic structure	1497:1521	the pyranosidic structure of the reducing sugar	1497:1543	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	4	62	theme	study	918:922	arg1	goal					905:908	The goal	901:908	The goal of this study	901:922	The goal of this study is to develop and optimize a glycan-functionalized tridimensional collagen-based hydrogel that will make contact with and modulate the differentiation of a primary neuronal culture.					
32433878	9	63	theme	delivery	2205:2212	arg1	systems					2214:2220	cell delivery systems	2200:2220	cell delivery systems in the central nervous system	2200:2250	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	5	64	theme	developed	1110:1118	arg1	system					1120:1125	The developed system	1106:1125	The developed system	1106:1125	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	6	65	with	amination	1420:1428	arg1	β-Glc					1482:1486	Gal(β1-4)β-Glc	1473:1486	Gal(β1-4)β-Glc	1473:1486	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	6	65	with	amination	1420:1428	arg1	maltose					1435:1441	maltose	1435:1441	maltose (Glc(α1-4)α-Glc)	1435:1458	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	6	65	with	amination	1420:1428	arg1	α-Glc					1453:1457	Glc(α1-4)α-Glc	1444:1457	Glc(α1-4)α-Glc	1444:1457	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	6	65	with	amination	1420:1428	arg1	lactose					1464:1470	lactose	1464:1470	lactose (Gal(β1-4)β-Glc)	1464:1487	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	2	66	theme	able	582:585	arg1	biomaterials					569:580	biomaterials	569:580	biomaterials able to recapitulate natural components	569:620	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	5	67	theme	expression	1324:1333	arg1	modulation					1303:1312	a differential modulation	1288:1312	a differential modulation of glycan expression at the tissue level	1288:1353	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	3	68	theme	therapeutic	765:775	arg1	effect					777:782	their therapeutic effect	759:782	their therapeutic effect	759:782	Collagen hydrogels have shown potential as a vehicle for the delivery of cells into the brain, and their therapeutic effect can be expanded by functionalizing them to address the glycosylation patterns altered with specific brain disorders.					
32433878	1	69	from	changes	205:211	arg1	profile					234:240	the glycosylation profile	216:240	the glycosylation profile of the cell surface and extracellular matrix (ECM) components	216:302	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	9	70	from	influence	2085:2093	arg1	expression					2154:2163	cellular sugar expression	2139:2163	cellular sugar expression	2139:2163	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	9	71	theme	central	2229:2235	arg1	system					2245:2250	the central nervous system	2225:2250	the central nervous system	2225:2250	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	0	72	theme	Primary	85:91	arg1	Culture					93:99	a Neuronal Primary Culture	74:99	a Neuronal Primary Culture	74:99	Glycan-Functionalized Collagen Hydrogels Modulate the Glycoenvironment of a Neuronal Primary Culture.					
32433878	6	73	dep	sacrificed	1554:1563	arg1	linker					1578:1583	a linker	1576:1583	a linker	1576:1583	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	4	74	theme	tridimensional	975:988	arg1	hydrogel					1005:1012	a glycan-functionalized tridimensional collagen-based hydrogel	951:1012	a glycan-functionalized tridimensional collagen-based hydrogel that will make contact with and modulate the differentiation of a primary neuronal culture	951:1103	The goal of this study is to develop and optimize a glycan-functionalized tridimensional collagen-based hydrogel that will make contact with and modulate the differentiation of a primary neuronal culture.					
32433878	5	75	theme	differential	1290:1301	arg1	modulation					1303:1312	a differential modulation	1288:1312	a differential modulation of glycan expression at the tissue level	1288:1353	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	1	76	dep	surface	254:260	arg1	components					293:302	components	293:302	components	293:302	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	6	77	theme	reductive	1410:1418	arg1	amination					1420:1428	one step reductive amination	1401:1428	one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker)	1401:1584	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	1	78	theme	CNS	319:321	arg1	disorders					323:331	CNS disorders	319:331	CNS disorders	319:331	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	6	79	theme	β-Gal	1604:1608	arg1	residues					1610:1617	the α-Glc and β-Gal residues	1590:1617	residues	1610:1617	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	3	80	theme	brain	884:888	arg1	disorders					890:898	specific brain disorders	875:898	specific brain disorders	875:898	Collagen hydrogels have shown potential as a vehicle for the delivery of cells into the brain, and their therapeutic effect can be expanded by functionalizing them to address the glycosylation patterns altered with specific brain disorders.					
32433878	2	81	theme	conditions	525:534	arg1	glyco-signature					471:485	the glyco-signature	467:485	the glyco-signature of the physiological and pathological conditions	467:534	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	1	82	dep	development	137:147	arg1	the					133:135	the	133:135	the	133:135	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	4	83	theme	glycan-functionalized	953:973	arg1	hydrogel					1005:1012	a glycan-functionalized tridimensional collagen-based hydrogel	951:1012	a glycan-functionalized tridimensional collagen-based hydrogel that will make contact with and modulate the differentiation of a primary neuronal culture	951:1103	The goal of this study is to develop and optimize a glycan-functionalized tridimensional collagen-based hydrogel that will make contact with and modulate the differentiation of a primary neuronal culture.					
32433878	0	84	theme	Collagen	22:29	arg1	Hydrogels					31:39	Glycan-Functionalized Collagen Hydrogels	0:39	Glycan-Functionalized Collagen Hydrogels	0:39	Glycan-Functionalized Collagen Hydrogels Modulate the Glycoenvironment of a Neuronal Primary Culture.					
32433878	5	85	theme	more	1141:1144	arg1	information					1146:1156	more information	1141:1156	more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level	1141:1353	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	1	86	from	disorders	323:331	arg1	evident					308:314	evident	308:314	evident	308:314	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	5	87	theme	native	1211:1216	arg1	profile					1234:1240	the native glyco-signature profile	1207:1240	the native glyco-signature profile during the process of cell differentiation	1207:1283	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	3	88	theme	Collagen	660:667	arg1	hydrogels					669:677	Collagen hydrogels	660:677	Collagen hydrogels	660:677	Collagen hydrogels have shown potential as a vehicle for the delivery of cells into the brain, and their therapeutic effect can be expanded by functionalizing them to address the glycosylation patterns altered with specific brain disorders.					
32433878	1	89	theme	matrix	280:285	arg1	profile					234:240	the glycosylation profile	216:240	the glycosylation profile of the cell surface and extracellular matrix (ECM) components	216:302	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32433878	9	90	theme	glycan	2098:2103	arg1	residues					2105:2112	glycan residues	2098:2112	glycan residues present in the matrix	2098:2134	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	7	91	theme	lectin	1854:1859	arg1	staining					1861:1868	lectin staining	1854:1868	lectin staining	1854:1868	The glycoconjugate biopolymers were used to formulate hydrogels that were chemically and biologically characterized, and the glyco-signature profile of a neuronal culture after hydrogel treatment was analyzed by lectin staining.					
32433878	4	92	theme	culture	1097:1103	arg1	differentiation					1059:1073	the differentiation	1055:1073	the differentiation of a primary neuronal culture	1055:1103	The goal of this study is to develop and optimize a glycan-functionalized tridimensional collagen-based hydrogel that will make contact with and modulate the differentiation of a primary neuronal culture.					
32433878	9	93	theme	present	2114:2120	arg1	residues					2105:2112	glycan residues	2098:2112	glycan residues present in the matrix	2098:2134	These results indicate the differential influence of glycan residues present in the matrix on cellular sugar expression and thus have potential to enhance cell delivery systems in the central nervous system.					
32433878	5	94	theme	differentiation	1269:1283	arg1	process					1253:1259	the process	1249:1259	the process of cell differentiation	1249:1283	The developed system would provide more information on how a glyco-engineered material can influence the native glyco-signature profile during the process of cell differentiation by a differential modulation of glycan expression at the tissue level.					
32433878	7	95	theme	hydrogel	1819:1826	arg1	treatment					1828:1836	hydrogel treatment	1819:1836	hydrogel treatment	1819:1836	The glycoconjugate biopolymers were used to formulate hydrogels that were chemically and biologically characterized, and the glyco-signature profile of a neuronal culture after hydrogel treatment was analyzed by lectin staining.					
32433878	2	96	theme	biomaterial	379:389	arg1	platforms					391:399	biomaterial platforms	379:399	biomaterial platforms	379:399	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	2	97	theme	Regenerative	334:345	arg1	strategies					362:371	Regenerative medicine-based strategies	334:371	Regenerative medicine-based strategies using biomaterial platforms	334:399	Regenerative medicine-based strategies using biomaterial platforms are being increasingly used to target these diseases and to study the glyco-signature of the physiological and pathological conditions, particularly through the use of biomaterials able to recapitulate natural components and physical organization of the ECM.					
32433878	6	98	theme	β1-4	1477:1480	arg1	β-Glc					1482:1486	Gal(β1-4)β-Glc	1473:1486	Gal(β1-4)β-Glc	1473:1486	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	6	98	theme	β1-4	1477:1480	arg1	lactose					1464:1470	lactose	1464:1470	lactose (Gal(β1-4)β-Glc)	1464:1487	To this purpose, collagen polymers underwent one step reductive amination with maltose (Glc(α1-4)α-Glc) and lactose (Gal(β1-4)β-Glc) so that the pyranosidic structure of the reducing sugar could be sacrificed (acting as a linker) and the α-Glc and β-Gal residues exposed, respectively.					
32433878	1	99	theme	glycosylation	220:232	arg1	profile					234:240	the glycosylation profile	216:240	the glycosylation profile of the cell surface and extracellular matrix (ECM) components	216:302	Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so changes in the glycosylation profile of the cell surface and extracellular matrix (ECM) components are evident in CNS disorders.					
32339456	1	0	theme	protein	337:343	arg1	cysteines					345:353	protein cysteines	337:353	protein cysteines	337:353	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	4	1	theme	α	779:779	arg1	aldehydes					795:803	thiol-reactive α,β-unsaturated aldehydes	764:803	thiol-reactive α,β-unsaturated aldehydes	764:803	In alkaline protein microenvironments, per-O-acetylated monosaccharides undergo base-promoted β-elimination to form thiol-reactive α,β-unsaturated aldehydes, which then react with cysteine residues via Michael addition.					
32339456	4	2	theme	thiol-reactive	764:777	arg1	aldehydes					795:803	thiol-reactive α,β-unsaturated aldehydes	764:803	thiol-reactive α,β-unsaturated aldehydes	764:803	In alkaline protein microenvironments, per-O-acetylated monosaccharides undergo base-promoted β-elimination to form thiol-reactive α,β-unsaturated aldehydes, which then react with cysteine residues via Michael addition.					
32339456	1	3	theme	unnatural	89:97	arg1	monosaccharides					99:113	Per-O-acetylated unnatural monosaccharides	72:113	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group	72:146	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	1	4	theme	per-O-acetylated	359:374	arg1	sugars					376:381	per-O-acetylated sugars	359:381	per-O-acetylated sugars	359:381	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	4	5	theme	cysteine	828:835	arg1	residues					837:844	cysteine residues	828:844	cysteine residues	828:844	In alkaline protein microenvironments, per-O-acetylated monosaccharides undergo base-promoted β-elimination to form thiol-reactive α,β-unsaturated aldehydes, which then react with cysteine residues via Michael addition.					
32339456	1	6	from	labeling	191:198	arg1	cells					214:218	live cells	209:218	live cells	209:218	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	3	7	theme	mechanistic	524:534	arg1	investigation					536:548	a detailed mechanistic investigation	513:548	a detailed mechanistic investigation	513:548	Here, we present a detailed mechanistic investigation, which unveils the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification.					
32339456	6	8	theme	elimination-addition	978:997	arg1	mechanism					999:1007	The elimination-addition mechanism	974:1007	The elimination-addition mechanism	974:1007	The elimination-addition mechanism guides us to develop 1,6-di-O-propionyl-N-azidoacetylgalactosamine (1,6-Pr2GalNAz) as an improved unnatural monosaccharide for MGL.					
32339456	5	9	theme	typical	954:960	arg1	glycosides					962:971	typical glycosides	954:971	typical glycosides	954:971	This S-glyco-modification produces 3-thiolated sugars in hemiacetal form, rather than typical glycosides.					
32339456	1	10	theme	bioorthogonal	128:140	arg1	group					142:146	a bioorthogonal group	126:146	a bioorthogonal group	126:146	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	1	11	theme	live	209:212	arg1	cells					214:218	live cells	209:218	live cells	209:218	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	5	12	theme	3-thiolated	903:913	arg1	sugars					915:920	3-thiolated sugars	903:920	3-thiolated sugars	903:920	This S-glyco-modification produces 3-thiolated sugars in hemiacetal form, rather than typical glycosides.					
32339456	3	13	theme	S-glycosylation	570:584	arg1	termed S-glyco-modification					619:645	the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification	565:645	the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification	565:645	Here, we present a detailed mechanistic investigation, which unveils the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification.					
32339456	4	14	theme	base-promoted	728:740	arg1	β-elimination					742:754	base-promoted β-elimination	728:754	base-promoted β-elimination	728:754	In alkaline protein microenvironments, per-O-acetylated monosaccharides undergo base-promoted β-elimination to form thiol-reactive α,β-unsaturated aldehydes, which then react with cysteine residues via Michael addition.					
32339456	1	15	contain	containing	115:124	arg2	group					142:146	a bioorthogonal group	126:146	a bioorthogonal group	126:146	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	1	15	contain	containing	115:124	arg1	monosaccharides					99:113	Per-O-acetylated unnatural monosaccharides	72:113	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group	72:146	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	1	16	used	used	165:168	arg2	monosaccharides					99:113	Per-O-acetylated unnatural monosaccharides	72:113	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group	72:146	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	2	17	theme	nonspecific	427:437	arg1	reaction					439:446	this nonspecific reaction	422:446	this nonspecific reaction	422:446	While efforts are being made to avoid this nonspecific reaction in MGL, the reaction mechanism remains unknown.					
32339456	6	18	theme	improved	1098:1105	arg1	1,6-di-O-propionyl-N-azidoacetylgalactosamine					1030:1074	1,6-di-O-propionyl-N-azidoacetylgalactosamine	1030:1074	1,6-di-O-propionyl-N-azidoacetylgalactosamine (1,6-Pr2GalNAz)	1030:1090	The elimination-addition mechanism guides us to develop 1,6-di-O-propionyl-N-azidoacetylgalactosamine (1,6-Pr2GalNAz) as an improved unnatural monosaccharide for MGL.					
32339456	6	18	theme	improved	1098:1105	arg1	monosaccharide					1117:1130	an improved unnatural monosaccharide	1095:1130	an improved unnatural monosaccharide for MGL	1095:1138	The elimination-addition mechanism guides us to develop 1,6-di-O-propionyl-N-azidoacetylgalactosamine (1,6-Pr2GalNAz) as an improved unnatural monosaccharide for MGL.					
32339456	4	19	theme	Michael	850:856	arg1	addition					858:865	Michael addition	850:865	Michael addition	850:865	In alkaline protein microenvironments, per-O-acetylated monosaccharides undergo base-promoted β-elimination to form thiol-reactive α,β-unsaturated aldehydes, which then react with cysteine residues via Michael addition.					
32339456	4	20	theme	protein	660:666	arg1	microenvironments					668:684	alkaline protein microenvironments	651:684	alkaline protein microenvironments	651:684	In alkaline protein microenvironments, per-O-acetylated monosaccharides undergo base-promoted β-elimination to form thiol-reactive α,β-unsaturated aldehydes, which then react with cysteine residues via Michael addition.					
32339456	3	21	theme	atypical	596:603	arg1	termed S-glyco-modification					619:645	the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification	565:645	the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification	565:645	Here, we present a detailed mechanistic investigation, which unveils the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification.					
32339456	4	22	theme	per-O-acetylated	687:702	arg1	monosaccharides					704:718	per-O-acetylated monosaccharides	687:718	per-O-acetylated monosaccharides	687:718	In alkaline protein microenvironments, per-O-acetylated monosaccharides undergo base-promoted β-elimination to form thiol-reactive α,β-unsaturated aldehydes, which then react with cysteine residues via Michael addition.					
32339456	0	23	theme	Elimination-Addition	40:59	arg1	Mechanism					61:69	an Elimination-Addition Mechanism	37:69	an Elimination-Addition Mechanism	37:69	Protein S-Glyco-Modification through an Elimination-Addition Mechanism.					
32339456	1	24	theme	artificial	300:309	arg1	"					327:327	an artificial "S-glycosylation"	297:327	an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars	297:381	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	3	25	theme	glycosylation	605:617	arg1	termed S-glyco-modification					619:645	the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification	565:645	the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification	565:645	Here, we present a detailed mechanistic investigation, which unveils the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification.					
32339456	2	26	theme	reaction	460:467	arg1	mechanism					469:477	the reaction mechanism	456:477	the reaction mechanism	456:477	While efforts are being made to avoid this nonspecific reaction in MGL, the reaction mechanism remains unknown.					
32339456	6	27	theme	unnatural	1107:1115	arg1	1,6-di-O-propionyl-N-azidoacetylgalactosamine					1030:1074	1,6-di-O-propionyl-N-azidoacetylgalactosamine	1030:1074	1,6-di-O-propionyl-N-azidoacetylgalactosamine (1,6-Pr2GalNAz)	1030:1090	The elimination-addition mechanism guides us to develop 1,6-di-O-propionyl-N-azidoacetylgalactosamine (1,6-Pr2GalNAz) as an improved unnatural monosaccharide for MGL.					
32339456	6	27	theme	unnatural	1107:1115	arg1	monosaccharide					1117:1130	an improved unnatural monosaccharide	1095:1130	an improved unnatural monosaccharide for MGL	1095:1138	The elimination-addition mechanism guides us to develop 1,6-di-O-propionyl-N-azidoacetylgalactosamine (1,6-Pr2GalNAz) as an improved unnatural monosaccharide for MGL.					
32339456	3	28	theme	"	585:585	arg1	termed S-glyco-modification					619:645	the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification	565:645	the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification	565:645	Here, we present a detailed mechanistic investigation, which unveils the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification.					
32339456	1	29	theme	S-glycosylation	312:326	arg1	"					327:327	an artificial "S-glycosylation"	297:327	an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars	297:381	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	3	30	theme	being an	587:594	arg1	termed S-glyco-modification					619:645	the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification	565:645	the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification	565:645	Here, we present a detailed mechanistic investigation, which unveils the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification.					
32339456	1	31	theme	"	327:327	arg1	existence					284:292	the existence	280:292	the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars	280:381	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	4	32	dep	α	779:779	arg1	β-unsaturated					781:793	β-unsaturated	781:793	β-unsaturated	781:793	In alkaline protein microenvironments, per-O-acetylated monosaccharides undergo base-promoted β-elimination to form thiol-reactive α,β-unsaturated aldehydes, which then react with cysteine residues via Michael addition.					
32339456	1	33	theme	metabolic	174:182	arg1	labeling					191:198	metabolic glycan labeling	174:198	metabolic glycan labeling (MGL) in live cells	174:218	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	1	33	theme	metabolic	174:182	arg1	MGL					201:203	MGL	201:203	MGL	201:203	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	5	34	theme	hemiacetal	925:934	arg1	form					936:939	hemiacetal form	925:939	hemiacetal form	925:939	This S-glyco-modification produces 3-thiolated sugars in hemiacetal form, rather than typical glycosides.					
32339456	1	35	theme	glycan	184:189	arg1	labeling					191:198	metabolic glycan labeling	174:198	metabolic glycan labeling (MGL) in live cells	174:218	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	1	35	theme	glycan	184:189	arg1	MGL					201:203	MGL	201:203	MGL	201:203	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	4	36	theme	alkaline	651:658	arg1	microenvironments					668:684	alkaline protein microenvironments	651:684	alkaline protein microenvironments	651:684	In alkaline protein microenvironments, per-O-acetylated monosaccharides undergo base-promoted β-elimination to form thiol-reactive α,β-unsaturated aldehydes, which then react with cysteine residues via Michael addition.					
32339456	1	37	theme	Per-O-acetylated	72:87	arg1	monosaccharides					99:113	Per-O-acetylated unnatural monosaccharides	72:113	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group	72:146	Per-O-acetylated unnatural monosaccharides containing a bioorthogonal group have been widely used for metabolic glycan labeling (MGL) in live cells for two decades, but it is only recently that we discovered the existence of an artificial "S-glycosylation" between protein cysteines and per-O-acetylated sugars.					
32339456	3	38	theme	detailed	515:522	arg1	investigation					536:548	a detailed mechanistic investigation	513:548	a detailed mechanistic investigation	513:548	Here, we present a detailed mechanistic investigation, which unveils the "S-glycosylation" being an atypical glycosylation termed S-glyco-modification.					
33979146	4	0	theme	synthetic	758:766	arg1	glycopeptides					768:780	synthetic glycopeptides	758:780	synthetic glycopeptides	758:780	Our studies demonstrated the possibility of using synthetic glycopeptides as the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin.					
33979146	4	0	theme	synthetic	758:766	arg1	adiponectin					789:799	the adiponectin	785:799	the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin	785:915	Our studies demonstrated the possibility of using synthetic glycopeptides as the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin.					
33979146	1	1	theme	stereoselective	271:285	arg1	synthesis					294:302	stereoselective glycan synthesis	271:302	stereoselective glycan synthesis	271:302	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	2	2	theme	glycan	408:413	arg1	pattern					415:421	glycan pattern	408:421	glycan pattern	408:421	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	1	3	theme	glycan	287:292	arg1	synthesis					294:302	stereoselective glycan synthesis	271:302	stereoselective glycan synthesis	271:302	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	1	4	gly	glycosylated	110:121	arg1	domains					161:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains	92:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms	92:213	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	1	4	gly	glycosylated	110:121	arg1	ACDs					170:173	ACDs	170:173	ACDs	170:173	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	3	5	theme	different	585:593	arg1	models					601:606	different mouse models	585:606	different mouse models	585:606	In particular, hAdn-WM6877 was tested in detail with different mouse models and it exhibited promising in vivo antitumor, insulin sensitizing, and hepatoprotective activities.					
33979146	3	6	theme	mouse	595:599	arg1	models					601:606	different mouse models	585:606	different mouse models	585:606	In particular, hAdn-WM6877 was tested in detail with different mouse models and it exhibited promising in vivo antitumor, insulin sensitizing, and hepatoprotective activities.					
33979146	1	7	with	domains	161:167	arg1	glycoforms					204:213	the possible 15 glycoforms	188:213	the possible 15 glycoforms	188:213	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	1	7	with	domains	161:167	arg1	all					181:183	all	181:183	all	181:183	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	4	8	theme	therapeutics	848:859	arg1	development					827:837	the development	823:837	the development of novel therapeutics to treat diseases associated with deficient adiponectin	823:915	Our studies demonstrated the possibility of using synthetic glycopeptides as the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin.					
33979146	4	9	theme	novel	842:846	arg1	therapeutics					848:859	novel therapeutics	842:859	novel therapeutics	842:859	Our studies demonstrated the possibility of using synthetic glycopeptides as the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin.					
33979146	3	10	dep	in	635:636	arg1	vivo					638:641	vivo	638:641	vivo	638:641	In particular, hAdn-WM6877 was tested in detail with different mouse models and it exhibited promising in vivo antitumor, insulin sensitizing, and hepatoprotective activities.					
33979146	4	11	theme	mimetic	811:817	arg1	glycopeptides					768:780	synthetic glycopeptides	758:780	synthetic glycopeptides	758:780	Our studies demonstrated the possibility of using synthetic glycopeptides as the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin.					
33979146	4	11	theme	mimetic	811:817	arg1	adiponectin					789:799	the adiponectin	785:799	the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin	785:915	Our studies demonstrated the possibility of using synthetic glycopeptides as the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin.					
33979146	1	12	theme	possible	192:199	arg1	glycoforms					204:213	the possible 15 glycoforms	188:213	the possible 15 glycoforms	188:213	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	0	13	theme	Chemical	0:7	arg1	Synthesis					9:17	Chemical Synthesis	0:17	Chemical Synthesis	0:17	Chemical Synthesis and Biological Evaluations of Adiponectin Collagenous Domain Glycoforms.					
33979146	1	14	theme	chemical	308:315	arg1	ligation					325:332	chemical peptide ligation	308:332	chemical peptide ligation	308:332	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	1	15	theme	glycosylated	110:121	arg1	domains					161:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains	92:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms	92:213	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	1	15	theme	glycosylated	110:121	arg1	ACDs					170:173	ACDs	170:173	ACDs	170:173	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	0	16	theme	Biological	23:32	arg1	Evaluations					34:44	Biological Evaluations	23:44	Biological Evaluations	23:44	Chemical Synthesis and Biological Evaluations of Adiponectin Collagenous Domain Glycoforms.					
33979146	1	17	theme	peptide	317:323	arg1	ligation					325:332	chemical peptide ligation	308:332	chemical peptide ligation	308:332	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	4	18	gly	glycopeptides	768:780	arg2	adiponectin					789:799	the adiponectin	785:799	the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin	785:915	Our studies demonstrated the possibility of using synthetic glycopeptides as the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin.					
33979146	4	18	gly	glycopeptides	768:780	arg2	glycopeptides					768:780	synthetic glycopeptides	758:780	synthetic glycopeptides	758:780	Our studies demonstrated the possibility of using synthetic glycopeptides as the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin.					
33979146	1	19	theme	76-amino	123:130	arg1	domains					161:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains	92:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms	92:213	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	1	19	theme	76-amino	123:130	arg1	ACDs					170:173	ACDs	170:173	ACDs	170:173	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	2	20	theme	biological	349:358	arg1	studies					380:386	The following biological and pharmacological studies	335:386	The following biological and pharmacological studies	335:386	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	1	21	theme	acid	132:135	arg1	domains					161:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains	92:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms	92:213	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	1	21	theme	acid	132:135	arg1	ACDs					170:173	ACDs	170:173	ACDs	170:173	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	2	22	theme	systemic	504:511	arg1	metabolism					520:529	systemic energy metabolism	504:529	systemic energy metabolism	504:529	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	2	23	theme	growth	468:473	arg1	inhibition					442:451	the inhibition	438:451	the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism	438:529	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	2	23	theme	growth	468:473	arg1	regulation					490:499	the regulation	486:499	the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism	438:529	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	2	24	theme	metabolism	520:529	arg1	inhibition					442:451	the inhibition	438:451	the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism	438:529	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	2	24	theme	metabolism	520:529	arg1	regulation					490:499	the regulation	486:499	the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism	438:529	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	4	25	theme	deficient	895:903	arg1	adiponectin					905:915	deficient adiponectin	895:915	deficient adiponectin	895:915	Our studies demonstrated the possibility of using synthetic glycopeptides as the adiponectin downsized mimetic for the development of novel therapeutics to treat diseases associated with deficient adiponectin.					
33979146	2	26	theme	cell	463:466	arg1	growth					468:473	cancer cell growth	456:473	cancer cell growth	456:473	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	2	27	theme	energy	513:518	arg1	metabolism					520:529	systemic energy metabolism	504:529	systemic energy metabolism	504:529	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	2	28	theme	cancer	456:461	arg1	growth					468:473	cancer cell growth	456:473	cancer cell growth	456:473	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	0	29	theme	Collagenous	61:71	arg1	Glycoforms					80:89	Adiponectin Collagenous Domain Glycoforms	49:89	Adiponectin Collagenous Domain Glycoforms	49:89	Chemical Synthesis and Biological Evaluations of Adiponectin Collagenous Domain Glycoforms.					
33979146	3	30	theme	hepatoprotective	679:694	arg1	activities					696:705	hepatoprotective activities	679:705	hepatoprotective activities	679:705	In particular, hAdn-WM6877 was tested in detail with different mouse models and it exhibited promising in vivo antitumor, insulin sensitizing, and hepatoprotective activities.					
33979146	3	31	theme	promising	625:633	arg1	antitumor					643:651	promising in vivo antitumor	625:651	promising in vivo antitumor	625:651	In particular, hAdn-WM6877 was tested in detail with different mouse models and it exhibited promising in vivo antitumor, insulin sensitizing, and hepatoprotective activities.					
33979146	1	32	theme	adiponectin	137:147	arg1	domains					161:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains	92:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms	92:213	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	1	32	theme	adiponectin	137:147	arg1	ACDs					170:173	ACDs	170:173	ACDs	170:173	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	0	33	theme	Adiponectin	49:59	arg1	Glycoforms					80:89	Adiponectin Collagenous Domain Glycoforms	49:89	Adiponectin Collagenous Domain Glycoforms	49:89	Chemical Synthesis and Biological Evaluations of Adiponectin Collagenous Domain Glycoforms.					
33979146	3	34	theme	in	635:636	arg1	antitumor					643:651	promising in vivo antitumor	625:651	promising in vivo antitumor	625:651	In particular, hAdn-WM6877 was tested in detail with different mouse models and it exhibited promising in vivo antitumor, insulin sensitizing, and hepatoprotective activities.					
33979146	1	35	theme	collagenous	149:159	arg1	domains					161:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains	92:167	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms	92:213	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	1	35	theme	collagenous	149:159	arg1	ACDs					170:173	ACDs	170:173	ACDs	170:173	The homogeneously glycosylated 76-amino acid adiponectin collagenous domains (ACDs) with all of the possible 15 glycoforms have been chemically and individually synthesized using stereoselective glycan synthesis and chemical peptide ligation.					
33979146	3	36	theme	sensitizing	662:672	arg1	insulin					654:660	insulin sensitizing	654:672	insulin sensitizing	654:672	In particular, hAdn-WM6877 was tested in detail with different mouse models and it exhibited promising in vivo antitumor, insulin sensitizing, and hepatoprotective activities.					
33979146	2	37	theme	following	339:347	arg1	studies					380:386	The following biological and pharmacological studies	335:386	The following biological and pharmacological studies	335:386	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	2	38	theme	pharmacological	364:378	arg1	studies					380:386	The following biological and pharmacological studies	335:386	The following biological and pharmacological studies	335:386	The following biological and pharmacological studies enabled correlating glycan pattern to function in the inhibition of cancer cell growth as well as the regulation of systemic energy metabolism.					
33979146	0	39	theme	Glycoforms	80:89	arg1	Synthesis					9:17	Chemical Synthesis	0:17	Chemical Synthesis	0:17	Chemical Synthesis and Biological Evaluations of Adiponectin Collagenous Domain Glycoforms.					
33979146	0	39	theme	Glycoforms	80:89	arg1	Evaluations					34:44	Biological Evaluations	23:44	Biological Evaluations	23:44	Chemical Synthesis and Biological Evaluations of Adiponectin Collagenous Domain Glycoforms.					
33979146	0	40	theme	Domain	73:78	arg1	Glycoforms					80:89	Adiponectin Collagenous Domain Glycoforms	49:89	Adiponectin Collagenous Domain Glycoforms	49:89	Chemical Synthesis and Biological Evaluations of Adiponectin Collagenous Domain Glycoforms.					
34380744	5	0	theme	HCC-specific	817:828	arg1	changes					846:852	HCC-specific N-glycosylation changes	817:852	HCC-specific N-glycosylation changes	817:852	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	4	1	theme	heterogeneous	729:741	arg1	nature					765:770	the heterogeneous genetic and molecular nature	725:770	the heterogeneous genetic and molecular nature of HCC	725:777	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	4	2	link	N-linked	662:669	arg1	glycosylation					671:683	the N-linked glycosylation	658:683	the N-linked glycosylation of HCC tumors	658:697	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	5	3	theme	HCC	904:906	arg1	samples					915:921	HCC tissue samples	904:921	HCC tissue samples	904:921	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	2	4	theme	targeted	383:390	arg1	therapies					392:400	certain molecular targeted therapies	365:400	certain molecular targeted therapies	365:400	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	4	5	theme	identified	624:633	arg1	changes					647:653	identified significant changes	624:653	identified significant changes in the N-linked glycosylation of HCC tumors	624:697	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	5	6	theme	N-glycosylation	830:844	arg1	changes					846:852	HCC-specific N-glycosylation changes	817:852	HCC-specific N-glycosylation changes	817:852	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	8	7	theme	targeted	1661:1668	arg1	therapies					1679:1687	targeted clinical therapies	1661:1687	targeted clinical therapies	1661:1687	IMPLICATIONS: Correlating N-glycosylation to specific subtypes offers the specific detection of subtypes of HCC, which could both enhance early HCC sensitivity and guide targeted clinical therapies.					
34380744	6	8	theme	nontumor	1224:1231	arg1	tissue					1233:1238	adjacent nontumor tissue	1215:1238	adjacent nontumor tissue	1215:1238	MALDI-IMS data displayed trends that could approximately distinguish between subtypes, with subtype 1 demonstrating significantly dysregulated N-glycosylation versus adjacent nontumor tissue.					
34380744	2	9	theme	molecular	373:381	arg1	therapies					392:400	certain molecular targeted therapies	365:400	certain molecular targeted therapies	365:400	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	4	10	from	changes	647:653	arg1	glycosylation					671:683	the N-linked glycosylation	658:683	the N-linked glycosylation of HCC tumors	658:697	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	7	11	gly	fucosylated	1427:1437	arg1	expression					1446:1455	fucosylated glycan expression	1427:1455	fucosylated glycan expression	1427:1455	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	7	12	theme	glycan	1439:1444	arg1	expression					1446:1455	fucosylated glycan expression	1427:1455	fucosylated glycan expression	1427:1455	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	2	13	theme	histologic	291:300	arg1	inter-					252:257	inter-	252:257	inter-	252:257	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	2	13	theme	histologic	291:300	arg1	subtypes/variants					319:335	histologic and/or molecular subtypes/variants	291:335	histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies	291:400	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	2	13	theme	histologic	291:300	arg1	heterogeneity					274:286	intratumor heterogeneity	263:286	intratumor heterogeneity	263:286	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	1	14	theme	Hepatocellular	83:96	arg1	carcinoma					98:106	Hepatocellular carcinoma	83:106	Hepatocellular carcinoma (HCC)	83:112	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	1	14	theme	Hepatocellular	83:96	arg1	cause					136:140	the second leading cause	117:140	the second leading cause of cancer deaths	117:157	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	1	14	theme	Hepatocellular	83:96	arg1	HCC					109:111	HCC	109:111	HCC	109:111	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	7	15	theme	N-glycan	1275:1282	arg1	structures					1284:1293	no individual N-glycan structures	1261:1293	no individual N-glycan structures that could identify specific subtypes	1261:1331	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	3	16	theme	tumor	585:589	arg1	heterogeneity					591:603	the tumor heterogeneity	581:603	the tumor heterogeneity	581:603	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	4	17	theme	significant	635:645	arg1	changes					647:653	identified significant changes	624:653	identified significant changes in the N-linked glycosylation of HCC tumors	624:697	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	1	18	theme	second	121:126	arg1	cause					136:140	the second leading cause	117:140	the second leading cause of cancer deaths	117:157	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	1	18	theme	second	121:126	arg1	carcinoma					98:106	Hepatocellular carcinoma	83:106	Hepatocellular carcinoma (HCC)	83:112	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	5	19	theme	tissue	908:913	arg1	samples					915:921	HCC tissue samples	904:921	HCC tissue samples	904:921	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	4	20	gly	glycosylation	671:683	arg1	tumors					692:697	HCC tumors	688:697	HCC tumors	688:697	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	3	21	theme	matrix-assisted	461:475	arg1	MALDI-IMS					532:540	MALDI-IMS	532:540	MALDI-IMS	532:540	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	21	theme	matrix-assisted	461:475	arg1	spectrometry					518:529	matrix-assisted laser desorption ionization imaging mass spectrometry	461:529	matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	461:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	22	theme	desorption	483:492	arg1	MALDI-IMS					532:540	MALDI-IMS	532:540	MALDI-IMS	532:540	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	22	theme	desorption	483:492	arg1	spectrometry					518:529	matrix-assisted laser desorption ionization imaging mass spectrometry	461:529	matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	461:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	23	theme	N-linked	435:442	arg1	glycosylation					444:456	N-linked glycosylation	435:456	N-linked glycosylation	435:456	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	24	theme	glycosylation	444:456	arg1	profiling					422:430	Spatial HCC tissue profiling	403:430	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	403:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	24	theme	glycosylation	444:456	arg1	method					562:567	a new method	556:567	a new method to evaluate the tumor heterogeneity	556:603	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	2	25	theme	intratumor	263:272	arg1	inter-					252:257	inter-	252:257	inter-	252:257	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	2	25	theme	intratumor	263:272	arg1	subtypes/variants					319:335	histologic and/or molecular subtypes/variants	291:335	histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies	291:400	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	2	25	theme	intratumor	263:272	arg1	heterogeneity					274:286	intratumor heterogeneity	263:286	intratumor heterogeneity	263:286	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	3	26	theme	ionization	494:503	arg1	MALDI-IMS					532:540	MALDI-IMS	532:540	MALDI-IMS	532:540	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	26	theme	ionization	494:503	arg1	spectrometry					518:529	matrix-assisted laser desorption ionization imaging mass spectrometry	461:529	matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	461:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	27	theme	tissue	415:420	arg1	profiling					422:430	Spatial HCC tissue profiling	403:430	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	403:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	27	theme	tissue	415:420	arg1	method					562:567	a new method	556:567	a new method to evaluate the tumor heterogeneity	556:603	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	4	28	theme	Previous	606:613	arg1	work					615:618	Previous work	606:618	Previous work	606:618	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	8	29	theme	clinical	1670:1677	arg1	therapies					1679:1687	targeted clinical therapies	1661:1687	targeted clinical therapies	1661:1687	IMPLICATIONS: Correlating N-glycosylation to specific subtypes offers the specific detection of subtypes of HCC, which could both enhance early HCC sensitivity and guide targeted clinical therapies.					
34380744	1	30	theme	incidence	177:185	arg1	rate					187:190	the incidence rate	173:190	the incidence rate in the United States	173:211	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	0	31	theme	N-Glycosylation	0:14	arg1	Patterns					16:23	N-Glycosylation Patterns	0:23	N-Glycosylation Patterns	0:23	N-Glycosylation Patterns Correlate with Hepatocellular Carcinoma Genetic Subtypes.					
34380744	7	32	theme	branched	1378:1385	arg1	expression					1394:1403	branched glycan expression	1378:1403	branched glycan expression	1378:1403	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	8	33	theme	early	1629:1633	arg1	sensitivity					1639:1649	early HCC sensitivity	1629:1649	early HCC sensitivity	1629:1649	IMPLICATIONS: Correlating N-glycosylation to specific subtypes offers the specific detection of subtypes of HCC, which could both enhance early HCC sensitivity and guide targeted clinical therapies.					
34380744	7	34	theme	fucosylated	1427:1437	arg1	expression					1446:1455	fucosylated glycan expression	1427:1455	fucosylated glycan expression	1427:1455	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	3	35	theme	laser	477:481	arg1	MALDI-IMS					532:540	MALDI-IMS	532:540	MALDI-IMS	532:540	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	35	theme	laser	477:481	arg1	spectrometry					518:529	matrix-assisted laser desorption ionization imaging mass spectrometry	461:529	matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	461:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	36	theme	Spatial	403:409	arg1	profiling					422:430	Spatial HCC tissue profiling	403:430	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	403:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	36	theme	Spatial	403:409	arg1	method					562:567	a new method	556:567	a new method to evaluate the tumor heterogeneity	556:603	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	6	37	theme	MALDI-IMS	1049:1057	arg1	data					1059:1062	MALDI-IMS data	1049:1062	MALDI-IMS data	1049:1062	MALDI-IMS data displayed trends that could approximately distinguish between subtypes, with subtype 1 demonstrating significantly dysregulated N-glycosylation versus adjacent nontumor tissue.					
34380744	7	38	theme	expression	1394:1403	arg1	correlation					1363:1373	the correlation	1359:1373	the correlation of branched glycan expression to HCC	1359:1410	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	7	38	theme	expression	1394:1403	arg1	whole					1417:1421	whole	1417:1421	whole	1417:1421	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	2	39	theme	molecular	309:317	arg1	inter-					252:257	inter-	252:257	inter-	252:257	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	2	39	theme	molecular	309:317	arg1	subtypes/variants					319:335	histologic and/or molecular subtypes/variants	291:335	histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies	291:400	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	2	39	theme	molecular	309:317	arg1	heterogeneity					274:286	intratumor heterogeneity	263:286	intratumor heterogeneity	263:286	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	8	40	theme	specific	1565:1572	arg1	detection					1574:1582	the specific detection	1561:1582	the specific detection of subtypes of HCC, which could both enhance early HCC sensitivity and guide targeted clinical therapies	1561:1687	IMPLICATIONS: Correlating N-glycosylation to specific subtypes offers the specific detection of subtypes of HCC, which could both enhance early HCC sensitivity and guide targeted clinical therapies.					
34380744	4	41	theme	HCC	688:690	arg1	tumors					692:697	HCC tumors	688:697	HCC tumors	688:697	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	3	42	theme	HCC	411:413	arg1	profiling					422:430	Spatial HCC tissue profiling	403:430	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	403:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	42	theme	HCC	411:413	arg1	method					562:567	a new method	556:567	a new method to evaluate the tumor heterogeneity	556:603	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	4	43	theme	genetic	743:749	arg1	nature					765:770	the heterogeneous genetic and molecular nature	725:770	the heterogeneous genetic and molecular nature of HCC	725:777	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	3	44	theme	new	558:560	arg1	profiling					422:430	Spatial HCC tissue profiling	403:430	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	403:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	44	theme	new	558:560	arg1	method					562:567	a new method	556:567	a new method to evaluate the tumor heterogeneity	556:603	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	0	45	theme	Carcinoma	55:63	arg1	Subtypes					73:80	Hepatocellular Carcinoma Genetic Subtypes	40:80	Hepatocellular Carcinoma Genetic Subtypes	40:80	N-Glycosylation Patterns Correlate with Hepatocellular Carcinoma Genetic Subtypes.					
34380744	4	46	theme	molecular	755:763	arg1	nature					765:770	the heterogeneous genetic and molecular nature	725:770	the heterogeneous genetic and molecular nature of HCC	725:777	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	3	47	theme	imaging	505:511	arg1	MALDI-IMS					532:540	MALDI-IMS	532:540	MALDI-IMS	532:540	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	47	theme	imaging	505:511	arg1	spectrometry					518:529	matrix-assisted laser desorption ionization imaging mass spectrometry	461:529	matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	461:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	7	48	theme	subtype	1460:1466	arg1	tumors					1470:1475	subtype 1 tumors	1460:1475	subtype 1 tumors	1460:1475	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	1	49	theme	United	199:204	arg1	States					206:211	the United States	195:211	the United States	195:211	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	0	50	theme	Hepatocellular	40:53	arg1	Subtypes					73:80	Hepatocellular Carcinoma Genetic Subtypes	40:80	Hepatocellular Carcinoma Genetic Subtypes	40:80	N-Glycosylation Patterns Correlate with Hepatocellular Carcinoma Genetic Subtypes.					
34380744	3	51	link	N-linked	435:442	arg1	glycosylation					444:456	N-linked glycosylation	435:456	N-linked glycosylation	435:456	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	52	theme	mass	513:516	arg1	MALDI-IMS					532:540	MALDI-IMS	532:540	MALDI-IMS	532:540	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	3	52	theme	mass	513:516	arg1	spectrometry					518:529	matrix-assisted laser desorption ionization imaging mass spectrometry	461:529	matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS)	461:541	Spatial HCC tissue profiling of N-linked glycosylation by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) may serve as a new method to evaluate the tumor heterogeneity.					
34380744	5	53	theme	genetic/molecular	858:874	arg1	features					882:889	genetic/molecular tumor features	858:889	genetic/molecular tumor features	858:889	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	5	54	theme	spatial	957:963	arg1	N-glycosylation					965:979	the spatial N-glycosylation	953:979	the spatial N-glycosylation	953:979	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	5	55	theme	tumor	876:880	arg1	features					882:889	genetic/molecular tumor features	858:889	genetic/molecular tumor features	858:889	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	4	56	contain	has	620:622	arg2	changes					647:653	identified significant changes	624:653	identified significant changes in the N-linked glycosylation of HCC tumors	624:697	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	4	56	contain	has	620:622	arg1	work					615:618	Previous work	606:618	Previous work	606:618	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	2	57	theme	certain	365:371	arg1	therapies					392:400	certain molecular targeted therapies	365:400	certain molecular targeted therapies	365:400	Studies have identified inter- and intratumor heterogeneity as histologic and/or molecular subtypes/variants associated with response to certain molecular targeted therapies.					
34380744	0	58	theme	Genetic	65:71	arg1	Subtypes					73:80	Hepatocellular Carcinoma Genetic Subtypes	40:80	Hepatocellular Carcinoma Genetic Subtypes	40:80	N-Glycosylation Patterns Correlate with Hepatocellular Carcinoma Genetic Subtypes.					
34380744	6	59	theme	adjacent	1215:1222	arg1	tissue					1233:1238	adjacent nontumor tissue	1215:1238	adjacent nontumor tissue	1215:1238	MALDI-IMS data displayed trends that could approximately distinguish between subtypes, with subtype 1 demonstrating significantly dysregulated N-glycosylation versus adjacent nontumor tissue.					
34380744	7	60	theme	glycan	1387:1392	arg1	expression					1394:1403	branched glycan expression	1378:1403	branched glycan expression	1378:1403	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	1	61	theme	leading	128:134	arg1	cause					136:140	the second leading cause	117:140	the second leading cause of cancer deaths	117:157	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	1	61	theme	leading	128:134	arg1	carcinoma					98:106	Hepatocellular carcinoma	83:106	Hepatocellular carcinoma (HCC)	83:112	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	8	62	theme	HCC	1599:1601	arg1	subtypes					1587:1594	subtypes	1587:1594	subtypes	1587:1594	IMPLICATIONS: Correlating N-glycosylation to specific subtypes offers the specific detection of subtypes of HCC, which could both enhance early HCC sensitivity and guide targeted clinical therapies.					
34380744	5	63	theme	used	995:998	arg1	classification					1014:1027	a widely used HCC molecular classification	986:1027	a widely used HCC molecular classification (Hoshida subtypes)	986:1046	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	5	63	theme	used	995:998	arg1	subtypes					1038:1045	Hoshida subtypes	1030:1045	Hoshida subtypes	1030:1045	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	7	64	theme	individual	1264:1273	arg1	structures					1284:1293	no individual N-glycan structures	1261:1293	no individual N-glycan structures that could identify specific subtypes	1261:1331	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	4	65	theme	HCC	775:777	arg1	nature					765:770	the heterogeneous genetic and molecular nature	725:770	the heterogeneous genetic and molecular nature of HCC	725:777	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	5	66	theme	Hoshida	1030:1036	arg1	classification					1014:1027	a widely used HCC molecular classification	986:1027	a widely used HCC molecular classification (Hoshida subtypes)	986:1046	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	5	66	theme	Hoshida	1030:1036	arg1	subtypes					1038:1045	Hoshida subtypes	1030:1045	Hoshida subtypes	1030:1045	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	8	67	theme	HCC	1635:1637	arg1	sensitivity					1639:1649	early HCC sensitivity	1629:1649	early HCC sensitivity	1629:1649	IMPLICATIONS: Correlating N-glycosylation to specific subtypes offers the specific detection of subtypes of HCC, which could both enhance early HCC sensitivity and guide targeted clinical therapies.					
34380744	5	68	theme	molecular	1004:1012	arg1	classification					1014:1027	a widely used HCC molecular classification	986:1027	a widely used HCC molecular classification (Hoshida subtypes)	986:1046	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	5	68	theme	molecular	1004:1012	arg1	subtypes					1038:1045	Hoshida subtypes	1030:1045	Hoshida subtypes	1030:1045	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	5	69	theme	HCC	1000:1002	arg1	classification					1014:1027	a widely used HCC molecular classification	986:1027	a widely used HCC molecular classification (Hoshida subtypes)	986:1046	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	5	69	theme	HCC	1000:1002	arg1	subtypes					1038:1045	Hoshida subtypes	1030:1045	Hoshida subtypes	1030:1045	To determine the correlation between HCC-specific N-glycosylation changes and genetic/molecular tumor features, we profiled HCC tissue samples with MALDI-IMS and correlated the spatial N-glycosylation with a widely used HCC molecular classification (Hoshida subtypes).					
34380744	4	70	theme	tumors	692:697	arg1	glycosylation					671:683	the N-linked glycosylation	658:683	the N-linked glycosylation of HCC tumors	658:697	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
34380744	8	71	theme	specific	1536:1543	arg1	subtypes					1545:1552	specific subtypes	1536:1552	specific subtypes	1536:1552	IMPLICATIONS: Correlating N-glycosylation to specific subtypes offers the specific detection of subtypes of HCC, which could both enhance early HCC sensitivity and guide targeted clinical therapies.					
34380744	1	72	from	rate	187:190	arg1	States					206:211	the United States	195:211	the United States	195:211	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	1	73	theme	cancer	145:150	arg1	deaths					152:157	cancer deaths	145:157	cancer deaths	145:157	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	6	74	theme	dysregulated	1179:1190	arg1	N-glycosylation					1192:1206	significantly dysregulated N-glycosylation	1165:1206	significantly dysregulated N-glycosylation	1165:1206	MALDI-IMS data displayed trends that could approximately distinguish between subtypes, with subtype 1 demonstrating significantly dysregulated N-glycosylation versus adjacent nontumor tissue.					
34380744	8	75	theme	subtypes	1587:1594	arg1	detection					1574:1582	the specific detection	1561:1582	the specific detection of subtypes of HCC, which could both enhance early HCC sensitivity and guide targeted clinical therapies	1561:1687	IMPLICATIONS: Correlating N-glycosylation to specific subtypes offers the specific detection of subtypes of HCC, which could both enhance early HCC sensitivity and guide targeted clinical therapies.					
34380744	1	76	theme	deaths	152:157	arg1	cause					136:140	the second leading cause	117:140	the second leading cause of cancer deaths	117:157	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	1	76	theme	deaths	152:157	arg1	carcinoma					98:106	Hepatocellular carcinoma	83:106	Hepatocellular carcinoma (HCC)	83:112	Hepatocellular carcinoma (HCC) is the second leading cause of cancer deaths globally, and the incidence rate in the United States is increasing.					
34380744	7	77	theme	specific	1315:1322	arg1	subtypes					1324:1331	specific subtypes	1315:1331	specific subtypes	1315:1331	Although there were no individual N-glycan structures that could identify specific subtypes, trends emerged regarding the correlation of branched glycan expression to HCC as a whole and fucosylated glycan expression to subtype 1 tumors specifically.					
34380744	4	78	theme	N-linked	662:669	arg1	glycosylation					671:683	the N-linked glycosylation	658:683	the N-linked glycosylation of HCC tumors	658:697	Previous work has identified significant changes in the N-linked glycosylation of HCC tumors but has not accounted for the heterogeneous genetic and molecular nature of HCC.					
32928962	0	0	theme	galectin-3	76:85	arg1	crosslinking					96:107	galectin-3 mediated crosslinking	76:107	galectin-3 mediated crosslinking	76:107	Decrease of core 2 O-glycans on synovial lubricin in osteoarthritis reduces galectin-3 mediated crosslinking.					
32928962	8	1	theme	molecules	1505:1513	arg1	crosslinking					1474:1485	crosslinking	1474:1485	crosslinking of surface-active molecules in OA	1474:1519	These data suggest a defect in crosslinking of surface-active molecules in OA and provide novel insights into OA molecular pathology.					
32928962	1	2	link	O-linked	198:205	arg1	lubricin					142:149	The synovial fluid glycoprotein lubricin	110:149	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4)	110:180	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	1	2	link	O-linked	198:205	arg1	lubricant					231:239	lubricant	231:239	lubricant	231:239	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	6	3	theme	cells	1095:1099	arg1	transfection					1057:1068	transfection	1057:1068	transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein	1057:1172	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	7	4	from	Tn-antigens	1430:1440	arg1	T-antigens					1414:1423	T-antigens	1414:1423	T-antigens	1414:1423	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	7	4	from	Tn-antigens	1430:1440	arg1	O-glycans					1403:1411	unsialylated core 1 O-glycans	1383:1411	unsialylated core 1 O-glycans (T-antigens)	1383:1424	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	4	5	theme	glycans	697:703	arg1	spectrum					685:692	the spectrum	681:692	the spectrum of glycans	681:703	However, with the spectrum of glycans being found on lubricin, the glycan candidates involved in this interaction were unknown.					
32928962	5	6	link	O-linked	828:835	arg1	glycans					837:843	the core 2 O-linked glycans	817:843	the core 2 O-linked glycans	817:843	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	0	7	from	Decrease	0:7	arg1	osteoarthritis					53:66	osteoarthritis	53:66	osteoarthritis	53:66	Decrease of core 2 O-glycans on synovial lubricin in osteoarthritis reduces galectin-3 mediated crosslinking.					
32928962	0	7	from	Decrease	0:7	arg1	lubricin					41:48	synovial lubricin	32:48	synovial lubricin	32:48	Decrease of core 2 O-glycans on synovial lubricin in osteoarthritis reduces galectin-3 mediated crosslinking.					
32928962	2	8	gly	unsialylated	402:413	arg1	Tn-antigens					415:425	unsialylated Tn-antigens	402:425	unsialylated Tn-antigens	402:425	Lubricin's ability to reduce friction is related to its glycosylation consisting of sialylated and unsialylated Tn-antigens and core 1 and core 2 structures.					
32928962	3	9	theme	superficial	578:588	arg1	layer					590:594	the lubricating superficial layer	562:594	the lubricating superficial layer of cartilage	562:607	The glycans on lubricin have also been suggested to be involved in crosslinking and stabilization of the lubricating superficial layer of cartilage by mediating interaction between lubricin and galectin-3.					
32928962	6	10	theme	galectin-3	1194:1203	arg1	binding					1205:1211	increased galectin-3 binding	1184:1211	increased galectin-3 binding	1184:1211	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	0	11	theme	mediated	87:94	arg1	crosslinking					96:107	galectin-3 mediated crosslinking	76:107	galectin-3 mediated crosslinking	76:107	Decrease of core 2 O-glycans on synovial lubricin in osteoarthritis reduces galectin-3 mediated crosslinking.					
32928962	8	12	theme	novel	1533:1537	arg1	insights					1539:1546	novel insights	1533:1546	novel insights into OA molecular pathology	1533:1574	These data suggest a defect in crosslinking of surface-active molecules in OA and provide novel insights into OA molecular pathology.					
32928962	6	13	theme	increased	1184:1192	arg1	binding					1205:1211	increased galectin-3 binding	1184:1211	increased galectin-3 binding	1184:1211	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	7	14	theme	galectin-3	1251:1260	arg1	interactions					1262:1273	the galectin-3 interactions	1247:1273	the galectin-3 interactions with synovial lubricin	1247:1296	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	1	15	dep	lubricin	142:149	arg1	known					157:161	known	157:161	known as proteoglycan 4	157:179	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	1	16	theme	osteoarthritis	270:283	arg1	development					290:300	osteoarthritis (OA) development	270:300	osteoarthritis (OA) development	270:300	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	8	17	from	crosslinking	1474:1485	arg1	OA					1518:1519	OA	1518:1519	OA	1518:1519	These data suggest a defect in crosslinking of surface-active molecules in OA and provide novel insights into OA molecular pathology.					
32928962	5	18	theme	plasmon	908:914	arg1	resonance					916:924	surface plasmon resonance	900:924	surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3	900:1042	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	4	19	theme	glycan	734:739	arg1	candidates					741:750	the glycan candidates	730:750	the glycan candidates involved in this interaction	730:779	However, with the spectrum of glycans being found on lubricin, the glycan candidates involved in this interaction were unknown.					
32928962	4	19	theme	glycan	734:739	arg1	unknown					786:792	unknown	786:792	unknown	786:792	However, with the spectrum of glycans being found on lubricin, the glycan candidates involved in this interaction were unknown.					
32928962	5	20	theme	devoid	977:982	arg1	lubricin					959:966	recombinant lubricin	947:966	recombinant lubricin (rhPRG4) devoid of core 2 structures	947:1003	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	5	20	theme	devoid	977:982	arg1	rhPRG4					969:974	rhPRG4	969:974	rhPRG4	969:974	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	7	21	theme	late-stage	1328:1337	arg1	patients					1342:1349	late-stage OA patients	1328:1349	late-stage OA patients	1328:1349	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	6	22	theme	hamster	1081:1087	arg1	cells					1095:1099	Chinese hamster ovary cells	1073:1099	Chinese hamster ovary cells	1073:1099	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	6	23	theme	ovary	1089:1093	arg1	cells					1095:1099	Chinese hamster ovary cells	1073:1099	Chinese hamster ovary cells	1073:1099	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	6	24	theme	Chinese	1073:1079	arg1	cells					1095:1099	Chinese hamster ovary cells	1073:1099	Chinese hamster ovary cells	1073:1099	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	6	25	theme	mucin-type	1148:1157	arg1	O-glycoprotein					1159:1172	a mucin-type O-glycoprotein	1146:1172	a mucin-type O-glycoprotein	1146:1172	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	8	26	from	defect	1464:1469	arg1	crosslinking					1474:1485	crosslinking	1474:1485	crosslinking of surface-active molecules in OA	1474:1519	These data suggest a defect in crosslinking of surface-active molecules in OA and provide novel insights into OA molecular pathology.					
32928962	1	27	dep	known	157:161	arg1	also					152:155	also	152:155	also	152:155	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	7	28	from	increase	1371:1378	arg1	T-antigens					1414:1423	T-antigens	1414:1423	T-antigens	1414:1423	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	7	28	from	increase	1371:1378	arg1	O-glycans					1403:1411	unsialylated core 1 O-glycans	1383:1411	unsialylated core 1 O-glycans (T-antigens)	1383:1424	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	5	29	theme	lubricin-galectin-3	858:876	arg1	interaction					878:888	this lubricin-galectin-3 interaction	853:888	this lubricin-galectin-3 interaction	853:888	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	8	30	from	OA	1518:1519	arg1	crosslinking					1474:1485	crosslinking	1474:1485	crosslinking of surface-active molecules in OA	1474:1519	These data suggest a defect in crosslinking of surface-active molecules in OA and provide novel insights into OA molecular pathology.					
32928962	8	31	theme	molecular	1556:1564	arg1	pathology					1566:1574	OA molecular pathology	1553:1574	OA molecular pathology	1553:1574	These data suggest a defect in crosslinking of surface-active molecules in OA and provide novel insights into OA molecular pathology.					
32928962	3	32	theme	lubricating	566:576	arg1	layer					590:594	the lubricating superficial layer	562:594	the lubricating superficial layer of cartilage	562:607	The glycans on lubricin have also been suggested to be involved in crosslinking and stabilization of the lubricating superficial layer of cartilage by mediating interaction between lubricin and galectin-3.					
32928962	6	33	with	transfection	1057:1068	arg1	transferase					1124:1134	the core 2 GlcNAc transferase	1106:1134	the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein	1106:1172	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	8	34	theme	OA	1553:1554	arg1	pathology					1566:1574	OA molecular pathology	1553:1574	OA molecular pathology	1553:1574	These data suggest a defect in crosslinking of surface-active molecules in OA and provide novel insights into OA molecular pathology.					
32928962	5	35	theme	recombinant	1021:1031	arg1	galectin-3					1033:1042	recombinant galectin-3	1021:1042	recombinant galectin-3	1021:1042	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	5	36	theme	core	821:824	arg1	glycans					837:843	the core 2 O-linked glycans	817:843	the core 2 O-linked glycans	817:843	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	0	37	theme	core	12:15	arg1	O-glycans					19:27	core 2 O-glycans	12:27	core 2 O-glycans	12:27	Decrease of core 2 O-glycans on synovial lubricin in osteoarthritis reduces galectin-3 mediated crosslinking.					
32928962	5	38	theme	recombinant	947:957	arg1	lubricin					959:966	recombinant lubricin	947:966	recombinant lubricin (rhPRG4) devoid of core 2 structures	947:1003	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	5	38	theme	recombinant	947:957	arg1	rhPRG4					969:974	rhPRG4	969:974	rhPRG4	969:974	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	5	39	theme	surface	900:906	arg1	resonance					916:924	surface plasmon resonance	900:924	surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3	900:1042	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	5	40	theme	resonance	916:924	arg1	data					926:929	surface plasmon resonance data	900:929	surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3	900:1042	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	6	41	theme	GlcNAc	1117:1122	arg1	transferase					1124:1134	the core 2 GlcNAc transferase	1106:1134	the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein	1106:1172	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	7	42	theme	core	1396:1399	arg1	T-antigens					1414:1423	T-antigens	1414:1423	T-antigens	1414:1423	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	7	42	theme	core	1396:1399	arg1	O-glycans					1403:1411	unsialylated core 1 O-glycans	1383:1411	unsialylated core 1 O-glycans (T-antigens)	1383:1424	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	7	43	theme	OA	1339:1340	arg1	patients					1342:1349	late-stage OA patients	1328:1349	late-stage OA patients	1328:1349	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	5	44	theme	O-linked	828:835	arg1	glycans					837:843	the core 2 O-linked glycans	817:843	the core 2 O-linked glycans	817:843	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	0	45	theme	O-glycans	19:27	arg1	Decrease					0:7	Decrease	0:7	Decrease of core 2 O-glycans on synovial lubricin in osteoarthritis	0:66	Decrease of core 2 O-glycans on synovial lubricin in osteoarthritis reduces galectin-3 mediated crosslinking.					
32928962	1	46	gly	glycoprotein	129:140	arg1	glycoprotein					129:140	The synovial fluid glycoprotein lubricin	110:149	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4)	110:180	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	3	47	theme	cartilage	599:607	arg1	layer					590:594	the lubricating superficial layer	562:594	the lubricating superficial layer of cartilage	562:607	The glycans on lubricin have also been suggested to be involved in crosslinking and stabilization of the lubricating superficial layer of cartilage by mediating interaction between lubricin and galectin-3.					
32928962	7	48	theme	unsialylated	1383:1394	arg1	T-antigens					1414:1423	T-antigens	1414:1423	T-antigens	1414:1423	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	7	48	theme	unsialylated	1383:1394	arg1	O-glycans					1403:1411	unsialylated core 1 O-glycans	1383:1411	unsialylated core 1 O-glycans (T-antigens)	1383:1424	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	2	49	theme	unsialylated	402:413	arg1	Tn-antigens					415:425	unsialylated Tn-antigens	402:425	unsialylated Tn-antigens	402:425	Lubricin's ability to reduce friction is related to its glycosylation consisting of sialylated and unsialylated Tn-antigens and core 1 and core 2 structures.					
32928962	6	50	gly	O-glycoprotein	1159:1172	arg1	O-glycoprotein					1159:1172	a mucin-type O-glycoprotein	1146:1172	a mucin-type O-glycoprotein	1146:1172	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	2	51	dep	core	431:434	arg1	structures					449:458	structures	449:458	structures	449:458	Lubricin's ability to reduce friction is related to its glycosylation consisting of sialylated and unsialylated Tn-antigens and core 1 and core 2 structures.					
32928962	1	52	theme	synovial	114:121	arg1	lubricin					142:149	The synovial fluid glycoprotein lubricin	110:149	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4)	110:180	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	1	52	theme	synovial	114:121	arg1	lubricant					231:239	lubricant	231:239	lubricant	231:239	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	0	53	theme	synovial	32:39	arg1	lubricin					41:48	synovial lubricin	32:48	synovial lubricin	32:48	Decrease of core 2 O-glycans on synovial lubricin in osteoarthritis reduces galectin-3 mediated crosslinking.					
32928962	3	54	theme	layer	590:594	arg1	crosslinking					528:539	crosslinking	528:539	crosslinking	528:539	The glycans on lubricin have also been suggested to be involved in crosslinking and stabilization of the lubricating superficial layer of cartilage by mediating interaction between lubricin and galectin-3.					
32928962	3	54	theme	layer	590:594	arg1	stabilization					545:557	stabilization	545:557	stabilization	545:557	The glycans on lubricin have also been suggested to be involved in crosslinking and stabilization of the lubricating superficial layer of cartilage by mediating interaction between lubricin and galectin-3.					
32928962	1	55	theme	fluid	123:127	arg1	lubricin					142:149	The synovial fluid glycoprotein lubricin	110:149	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4)	110:180	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	1	55	theme	fluid	123:127	arg1	lubricant					231:239	lubricant	231:239	lubricant	231:239	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	7	56	with	level	1223:1227	arg1	lubricin					1289:1296	synovial lubricin	1280:1296	synovial lubricin	1280:1296	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	5	57	theme	core	987:990	arg1	structures					994:1003	core 2 structures	987:1003	core 2 structures	987:1003	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	3	58	from	glycans	465:471	arg1	lubricin					476:483	lubricin	476:483	lubricin	476:483	The glycans on lubricin have also been suggested to be involved in crosslinking and stabilization of the lubricating superficial layer of cartilage by mediating interaction between lubricin and galectin-3.					
32928962	1	59	theme	glycoprotein	129:140	arg1	lubricin					142:149	The synovial fluid glycoprotein lubricin	110:149	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4)	110:180	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	1	59	theme	glycoprotein	129:140	arg1	lubricant					231:239	lubricant	231:239	lubricant	231:239	The synovial fluid glycoprotein lubricin (also known as proteoglycan 4) is a mucin-type O-linked glycosylated biological lubricant implicated to be involved in osteoarthritis (OA) development.					
32928962	8	60	theme	surface-active	1490:1503	arg1	molecules					1505:1513	surface-active molecules	1490:1513	surface-active molecules in OA	1490:1519	These data suggest a defect in crosslinking of surface-active molecules in OA and provide novel insights into OA molecular pathology.					
32928962	6	61	theme	core	1110:1113	arg1	transferase					1124:1134	the core 2 GlcNAc transferase	1106:1134	the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein	1106:1172	Conversely, transfection of Chinese hamster ovary cells with the core 2 GlcNAc transferase acting on a mucin-type O-glycoprotein displayed increased galectin-3 binding.					
32928962	7	62	theme	synovial	1280:1287	arg1	lubricin					1289:1296	synovial lubricin	1280:1296	synovial lubricin	1280:1296	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	7	63	theme	galectin-3	1232:1241	arg1	interactions					1262:1273	the galectin-3 interactions	1247:1273	the galectin-3 interactions with synovial lubricin	1247:1296	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	7	63	theme	galectin-3	1232:1241	arg1	level					1223:1227	the level	1219:1227	the level of galectin-3	1219:1241	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	5	64	theme	structures	994:1003	arg1	devoid					977:982	devoid	977:982	devoid	977:982	Here, we confirm that the core 2 O-linked glycans mediate this lubricin-galectin-3 interaction, shown by surface plasmon resonance data indicating that recombinant lubricin (rhPRG4) devoid of core 2 structures did not bind to recombinant galectin-3.					
32928962	7	65	gly	unsialylated	1383:1394	arg1	T-antigens					1414:1423	T-antigens	1414:1423	T-antigens	1414:1423	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	7	65	gly	unsialylated	1383:1394	arg1	O-glycans					1403:1411	unsialylated core 1 O-glycans	1383:1411	unsialylated core 1 O-glycans (T-antigens)	1383:1424	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
32928962	4	66	located	found	711:715	arg2	spectrum					685:692	the spectrum	681:692	the spectrum of glycans	681:703	However, with the spectrum of glycans being found on lubricin, the glycan candidates involved in this interaction were unknown.					
32928962	4	66	located	found	711:715	arg1	lubricin					720:727	lubricin	720:727	lubricin	720:727	However, with the spectrum of glycans being found on lubricin, the glycan candidates involved in this interaction were unknown.					
32928962	8	67	from	molecules	1505:1513	arg1	OA					1518:1519	OA	1518:1519	OA	1518:1519	These data suggest a defect in crosslinking of surface-active molecules in OA and provide novel insights into OA molecular pathology.					
32928962	7	68	with	interactions	1262:1273	arg1	lubricin					1289:1296	synovial lubricin	1280:1296	synovial lubricin	1280:1296	Both the level of galectin-3 and the galectin-3 interactions with synovial lubricin were found to be decreased in late-stage OA patients, coinciding with an increase in unsialylated core 1 O-glycans (T-antigens) and Tn-antigens.					
33878353	0	0	theme	fruiting	96:103	arg1	body					105:108	the fruiting body	92:108	the fruiting body of Cordyceps militaris	92:131	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	0	1	from	characterization	14:29	arg1	body					105:108	the fruiting body	92:108	the fruiting body of Cordyceps militaris	92:131	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	10	2	contain	has	1265:1267	arg2	application					1281:1291	a potential application	1269:1291	a potential application	1269:1291	Collectively, CM3I and CM3II are different from the previously reported polysaccharides from C. militaris, and CM3II has a potential application in hypolipidemia and anti-atherosclerosis.					
33878353	10	2	contain	has	1265:1267	arg1	CM3II					1259:1263	CM3II	1259:1263	CM3II	1259:1263	Collectively, CM3I and CM3II are different from the previously reported polysaccharides from C. militaris, and CM3II has a potential application in hypolipidemia and anti-atherosclerosis.					
33878353	8	3	theme	lipid	991:995	arg1	levels					997:1002	plasma lipid levels	984:1002	plasma lipid levels in apolipoprotein E-deficient mice	984:1037	Moreover, CM3II exhibited powerful anti-atherosclerotic effects via lowering plasma lipid levels in apolipoprotein E-deficient mice.					
33878353	1	4	theme	atherosclerosis	171:185	arg1	cause					162:166	one major cause	152:166	one major cause	152:166	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	1	4	theme	atherosclerosis	171:185	arg1	change					218:223	a basic pathological change	197:223	a basic pathological change of cardiovascular diseases	197:250	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	7	5	theme	-β-D-Manp	834:842	arg1	glycosyls					855:863	-β-D-Manp(1 → linked glycosyls	834:863	→4)-β-D-Manp(1 → 6)-α-D-Manp(1 → 6)-β-D-Manp(1 → linked glycosyls	799:863	CM3II was characterized by its backbone, which was composed of →4)-β-D-Manp(1 → 6)-α-D-Manp(1 → 6)-β-D-Manp(1 → linked glycosyls, and especially the presence of O-methyl.					
33878353	3	6	theme	fruiting	399:406	arg1	body					408:411	the fruiting body	395:411	the fruiting body of C. militaris	395:427	In this study, alkaline-extracted polysaccharides were obtained from the fruiting body of C. militaris.					
33878353	0	7	from	Purification	0:11	arg1	body					105:108	the fruiting body	92:108	the fruiting body of Cordyceps militaris	92:131	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	8	8	theme	apolipoprotein	1007:1020	arg1	mice					1034:1037	apolipoprotein E-deficient mice	1007:1037	apolipoprotein E-deficient mice	1007:1037	Moreover, CM3II exhibited powerful anti-atherosclerotic effects via lowering plasma lipid levels in apolipoprotein E-deficient mice.					
33878353	8	9	theme	anti-atherosclerotic	942:961	arg1	effects					963:969	powerful anti-atherosclerotic effects	933:969	powerful anti-atherosclerotic effects	933:969	Moreover, CM3II exhibited powerful anti-atherosclerotic effects via lowering plasma lipid levels in apolipoprotein E-deficient mice.					
33878353	7	10	theme	O-methyl	897:904	arg1	presence					885:892	the presence	881:892	the presence of O-methyl	881:904	CM3II was characterized by its backbone, which was composed of →4)-β-D-Manp(1 → 6)-α-D-Manp(1 → 6)-β-D-Manp(1 → linked glycosyls, and especially the presence of O-methyl.					
33878353	9	11	theme	promoting	1089:1097	arg1	effect					1099:1104	its promoting effect	1085:1104	its promoting effect on LXRα and inhibitory effect on SREBP-2	1085:1145	The underlying mechanisms were attributed to its promoting effect on LXRα and inhibitory effect on SREBP-2.					
33878353	9	12	theme	underlying	1044:1053	arg1	mechanisms					1055:1064	The underlying mechanisms	1040:1064	The underlying mechanisms	1040:1064	The underlying mechanisms were attributed to its promoting effect on LXRα and inhibitory effect on SREBP-2.					
33878353	0	13	theme	militaris	123:131	arg1	body					105:108	the fruiting body	92:108	the fruiting body of Cordyceps militaris	92:131	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	8	14	theme	powerful	933:940	arg1	effects					963:969	powerful anti-atherosclerotic effects	933:969	powerful anti-atherosclerotic effects	933:969	Moreover, CM3II exhibited powerful anti-atherosclerotic effects via lowering plasma lipid levels in apolipoprotein E-deficient mice.					
33878353	2	15	theme	lipid-lowering	302:315	arg1	effects					317:323	lipid-lowering effects	302:323	lipid-lowering effects	302:323	Polysaccharide is a water-soluble component with lipid-lowering effects.					
33878353	0	16	theme	Cordyceps	113:121	arg1	militaris					123:131	Cordyceps militaris	113:131	Cordyceps militaris	113:131	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	0	17	from	effects	56:62	arg1	body					105:108	the fruiting body	92:108	the fruiting body of Cordyceps militaris	92:131	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	8	18	theme	E-deficient	1022:1032	arg1	mice					1034:1037	apolipoprotein E-deficient mice	1007:1037	apolipoprotein E-deficient mice	1007:1037	Moreover, CM3II exhibited powerful anti-atherosclerotic effects via lowering plasma lipid levels in apolipoprotein E-deficient mice.					
33878353	6	19	theme	glycosyls	725:733	arg1	presence					695:702	the presence	691:702	the presence of →4,6)β-D-Glcp(1 → glycosyls	691:733	CM3I was mainly composed of →4)α-D-Glcp(1 → glycosyls and differed from starch due to the presence of →4,6)β-D-Glcp(1 → glycosyls.					
33878353	3	20	theme	militaris	419:427	arg1	body					408:411	the fruiting body	395:411	the fruiting body of C. militaris	395:427	In this study, alkaline-extracted polysaccharides were obtained from the fruiting body of C. militaris.					
33878353	6	21	theme	→	723:723	arg1	glycosyls					725:733	→4,6)β-D-Glcp(1 → glycosyls	707:733	→4,6)β-D-Glcp(1 → glycosyls	707:733	CM3I was mainly composed of →4)α-D-Glcp(1 → glycosyls and differed from starch due to the presence of →4,6)β-D-Glcp(1 → glycosyls.					
33878353	8	22	theme	plasma	984:989	arg1	levels					997:1002	plasma lipid levels	984:1002	plasma lipid levels in apolipoprotein E-deficient mice	984:1037	Moreover, CM3II exhibited powerful anti-atherosclerotic effects via lowering plasma lipid levels in apolipoprotein E-deficient mice.					
33878353	5	23	theme	structural	520:529	arg1	characteristics					531:545	Their structural characteristics	514:545	Their structural characteristics	514:545	Their structural characteristics were investigated via chemical and spectroscopic methods.					
33878353	1	24	theme	basic	199:203	arg1	cause					162:166	one major cause	152:166	one major cause	152:166	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	1	24	theme	basic	199:203	arg1	change					218:223	a basic pathological change	197:223	a basic pathological change of cardiovascular diseases	197:250	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	3	25	theme	C.	416:417	arg1	militaris					419:427	C. militaris	416:427	C. militaris	416:427	In this study, alkaline-extracted polysaccharides were obtained from the fruiting body of C. militaris.					
33878353	8	26	from	levels	997:1002	arg1	mice					1034:1037	apolipoprotein E-deficient mice	1007:1037	apolipoprotein E-deficient mice	1007:1037	Moreover, CM3II exhibited powerful anti-atherosclerotic effects via lowering plasma lipid levels in apolipoprotein E-deficient mice.					
33878353	10	27	from	polysaccharides	1220:1234	arg1	different					1181:1189	different	1181:1189	different	1181:1189	Collectively, CM3I and CM3II are different from the previously reported polysaccharides from C. militaris, and CM3II has a potential application in hypolipidemia and anti-atherosclerosis.					
33878353	10	28	from	militaris	1244:1252	arg1	polysaccharides					1220:1234	the previously reported polysaccharides	1196:1234	the previously reported polysaccharides from C. militaris	1196:1252	Collectively, CM3I and CM3II are different from the previously reported polysaccharides from C. militaris, and CM3II has a potential application in hypolipidemia and anti-atherosclerosis.					
33878353	6	29	theme	β-D-Glcp	712:719	arg1	glycosyls					725:733	→4,6)β-D-Glcp(1 → glycosyls	707:733	→4,6)β-D-Glcp(1 → glycosyls	707:733	CM3I was mainly composed of →4)α-D-Glcp(1 → glycosyls and differed from starch due to the presence of →4,6)β-D-Glcp(1 → glycosyls.					
33878353	1	30	theme	pathological	205:216	arg1	cause					162:166	one major cause	152:166	one major cause	152:166	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	1	30	theme	pathological	205:216	arg1	change					218:223	a basic pathological change	197:223	a basic pathological change of cardiovascular diseases	197:250	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	4	31	theme	exclusion	488:496	arg1	chromatography					498:511	size exclusion chromatography	483:511	size exclusion chromatography	483:511	Polysaccharides were purified via anion exchange and size exclusion chromatography.					
33878353	10	32	theme	potential	1271:1279	arg1	application					1281:1291	a potential application	1269:1291	a potential application	1269:1291	Collectively, CM3I and CM3II are different from the previously reported polysaccharides from C. militaris, and CM3II has a potential application in hypolipidemia and anti-atherosclerosis.					
33878353	0	33	theme	anti-atherosclerotic	35:54	arg1	effects					56:62	anti-atherosclerotic effects	35:62	anti-atherosclerotic effects	35:62	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	9	34	from	effect	1099:1104	arg1	SREBP-2					1139:1145	SREBP-2	1139:1145	SREBP-2	1139:1145	The underlying mechanisms were attributed to its promoting effect on LXRα and inhibitory effect on SREBP-2.					
33878353	9	34	from	effect	1099:1104	arg1	LXRα					1109:1112	LXRα	1109:1112	LXRα	1109:1112	The underlying mechanisms were attributed to its promoting effect on LXRα and inhibitory effect on SREBP-2.					
33878353	9	34	from	effect	1099:1104	arg1	effect					1129:1134	inhibitory effect	1118:1134	inhibitory effect	1118:1134	The underlying mechanisms were attributed to its promoting effect on LXRα and inhibitory effect on SREBP-2.					
33878353	6	35	theme	α-D-Glcp	636:643	arg1	glycosyls					649:657	→4)α-D-Glcp(1 → glycosyls	633:657	→4)α-D-Glcp(1 → glycosyls	633:657	CM3I was mainly composed of →4)α-D-Glcp(1 → glycosyls and differed from starch due to the presence of →4,6)β-D-Glcp(1 → glycosyls.					
33878353	3	36	theme	alkaline-extracted	341:358	arg1	polysaccharides					360:374	alkaline-extracted polysaccharides	341:374	alkaline-extracted polysaccharides	341:374	In this study, alkaline-extracted polysaccharides were obtained from the fruiting body of C. militaris.					
33878353	2	37	with	component	287:295	arg1	effects					317:323	lipid-lowering effects	302:323	lipid-lowering effects	302:323	Polysaccharide is a water-soluble component with lipid-lowering effects.					
33878353	7	38	link	linked	848:853	arg1	glycosyls					855:863	-β-D-Manp(1 → linked glycosyls	834:863	→4)-β-D-Manp(1 → 6)-α-D-Manp(1 → 6)-β-D-Manp(1 → linked glycosyls	799:863	CM3II was characterized by its backbone, which was composed of →4)-β-D-Manp(1 → 6)-α-D-Manp(1 → 6)-β-D-Manp(1 → linked glycosyls, and especially the presence of O-methyl.					
33878353	4	39	theme	anion	464:468	arg1	exchange					470:477	anion exchange	464:477	anion exchange	464:477	Polysaccharides were purified via anion exchange and size exclusion chromatography.					
33878353	5	40	dep	chemical	569:576	arg1	methods					596:602	methods	596:602	methods	596:602	Their structural characteristics were investigated via chemical and spectroscopic methods.					
33878353	10	41	theme	reported	1211:1218	arg1	polysaccharides					1220:1234	the previously reported polysaccharides	1196:1234	the previously reported polysaccharides from C. militaris	1196:1252	Collectively, CM3I and CM3II are different from the previously reported polysaccharides from C. militaris, and CM3II has a potential application in hypolipidemia and anti-atherosclerosis.					
33878353	1	42	theme	cardiovascular	228:241	arg1	diseases					243:250	cardiovascular diseases	228:250	cardiovascular diseases	228:250	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	9	43	theme	inhibitory	1118:1127	arg1	effect					1129:1134	inhibitory effect	1118:1134	inhibitory effect	1118:1134	The underlying mechanisms were attributed to its promoting effect on LXRα and inhibitory effect on SREBP-2.					
33878353	4	44	theme	size	483:486	arg1	chromatography					498:511	size exclusion chromatography	483:511	size exclusion chromatography	483:511	Polysaccharides were purified via anion exchange and size exclusion chromatography.					
33878353	2	45	theme	water-soluble	273:285	arg1	Polysaccharide					253:266	Polysaccharide	253:266	Polysaccharide	253:266	Polysaccharide is a water-soluble component with lipid-lowering effects.					
33878353	2	45	theme	water-soluble	273:285	arg1	component					287:295	a water-soluble component	271:295	a water-soluble component with lipid-lowering effects	271:323	Polysaccharide is a water-soluble component with lipid-lowering effects.					
33878353	7	46	theme	linked	848:853	arg1	glycosyls					855:863	-β-D-Manp(1 → linked glycosyls	834:863	→4)-β-D-Manp(1 → 6)-α-D-Manp(1 → 6)-β-D-Manp(1 → linked glycosyls	799:863	CM3II was characterized by its backbone, which was composed of →4)-β-D-Manp(1 → 6)-α-D-Manp(1 → 6)-β-D-Manp(1 → linked glycosyls, and especially the presence of O-methyl.					
33878353	1	47	theme	diseases	243:250	arg1	cause					162:166	one major cause	152:166	one major cause	152:166	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	1	47	theme	diseases	243:250	arg1	change					218:223	a basic pathological change	197:223	a basic pathological change of cardiovascular diseases	197:250	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	0	48	from	body	105:108	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	0	48	from	body	105:108	arg1	effects					56:62	anti-atherosclerotic effects	35:62	anti-atherosclerotic effects	35:62	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	0	48	from	body	105:108	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	0	48	from	body	105:108	arg1	polysaccharides					71:85	the polysaccharides	67:85	the polysaccharides from the fruiting body of Cordyceps militaris	67:131	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	1	49	theme	major	156:160	arg1	cause					162:166	one major cause	152:166	one major cause	152:166	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	1	49	theme	major	156:160	arg1	change					218:223	a basic pathological change	197:223	a basic pathological change of cardiovascular diseases	197:250	Hyperlipidemia is one major cause of atherosclerosis, which is a basic pathological change of cardiovascular diseases.					
33878353	0	50	theme	polysaccharides	71:85	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	0	50	theme	polysaccharides	71:85	arg1	effects					56:62	anti-atherosclerotic effects	35:62	anti-atherosclerotic effects	35:62	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	0	50	theme	polysaccharides	71:85	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and anti-atherosclerotic effects of the polysaccharides from the fruiting body of Cordyceps militaris.					
33878353	7	51	dep	-β-D-Manp	802:810	arg1	→4					799:800	→4	799:800	→4	799:800	CM3II was characterized by its backbone, which was composed of →4)-β-D-Manp(1 → 6)-α-D-Manp(1 → 6)-β-D-Manp(1 → linked glycosyls, and especially the presence of O-methyl.					
33878353	7	51	dep	-β-D-Manp	802:810	arg1	glycosyls					855:863	-β-D-Manp(1 → linked glycosyls	834:863	→4)-β-D-Manp(1 → 6)-α-D-Manp(1 → 6)-β-D-Manp(1 → linked glycosyls	799:863	CM3II was characterized by its backbone, which was composed of →4)-β-D-Manp(1 → 6)-α-D-Manp(1 → 6)-β-D-Manp(1 → linked glycosyls, and especially the presence of O-methyl.					
33878353	6	52	theme	→	647:647	arg1	glycosyls					649:657	→4)α-D-Glcp(1 → glycosyls	633:657	→4)α-D-Glcp(1 → glycosyls	633:657	CM3I was mainly composed of →4)α-D-Glcp(1 → glycosyls and differed from starch due to the presence of →4,6)β-D-Glcp(1 → glycosyls.					
33077685	2	0	gly	N-glycosylation	298:312	arg1	level					334:338	the level	330:338	the level of intact glycopeptides	330:362	Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.					
33077685	2	0	gly	N-glycosylation	298:312	arg2	level					334:338	the level	330:338	the level of intact glycopeptides	330:362	Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.					
33077685	2	0	gly	N-glycosylation	298:312	arg1	protein					319:325	S protein	317:325	S protein	317:325	Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.					
33077685	6	1	theme	cell-expressed	726:739	arg1	protein					743:749	the insect cell-expressed S protein	715:749	the insect cell-expressed S protein	715:749	In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type.					
33077685	1	2	theme	syndrome	159:166	arg1	SARS-CoV-2					181:190	SARS-CoV-2	181:190	SARS-CoV-2	181:190	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	1	2	theme	syndrome	159:166	arg1	coronavirus					168:178	severe acute respiratory syndrome coronavirus	134:178	severe acute respiratory syndrome coronavirus (SARS-CoV-2)	134:191	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	4	3	theme	protein	599:605	arg1	subunit					607:613	a human cell-expressed protein subunit	576:613	a human cell-expressed protein subunit	576:613	The glycosites exhibited glycoform heterogeneity as expected for a human cell-expressed protein subunit.					
33077685	1	4	gly	glycoprotein	93:104	arg1	glycoprotein					93:104	The glycoprotein spike (S)	89:114	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2)	89:191	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	9	5	theme	occupied	1175:1182	arg1	diverse					1199:1205	diverse	1199:1205	diverse	1199:1205	Our study indicates that the S protein N-glycosylation occurs regularly at each site, albeit the occupied N-glycans were diverse and heterogenous.					
33077685	9	5	theme	occupied	1175:1182	arg1	N-glycans					1184:1192	the occupied N-glycans	1171:1192	the occupied N-glycans	1171:1192	Our study indicates that the S protein N-glycosylation occurs regularly at each site, albeit the occupied N-glycans were diverse and heterogenous.					
33077685	1	6	theme	coronavirus	168:178	arg1	surface					123:129	the surface	119:129	the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2)	119:191	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	4	7	theme	cell-expressed	584:597	arg1	subunit					607:613	a human cell-expressed protein subunit	576:613	a human cell-expressed protein subunit	576:613	The glycosites exhibited glycoform heterogeneity as expected for a human cell-expressed protein subunit.					
33077685	10	8	theme	targeted	1439:1446	arg1	drugs					1448:1452	targeted drugs	1439:1452	targeted drugs	1439:1452	This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.					
33077685	2	9	theme	site-specific	284:296	arg1	N-glycosylation					298:312	the site-specific N-glycosylation	280:312	the site-specific N-glycosylation of S protein at the level of intact glycopeptides	280:362	Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.					
33077685	7	10	theme	differential	885:896	arg1	processing					898:907	the differential processing	881:907	the differential processing of N-glycans among human and insect cells	881:949	Our results revealed that the glycan types were highly determined by the differential processing of N-glycans among human and insect cells, regardless of the glycosites' location.					
33077685	8	11	theme	different	1049:1057	arg1	sizes					1059:1063	different sizes	1049:1063	different sizes of subunits	1049:1075	Moreover, the N-glycan compositions were conserved among different sizes of subunits.					
33077685	2	12	theme	glycopeptides	350:362	arg1	level					334:338	the level	330:338	the level of intact glycopeptides	330:362	Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.					
33077685	6	13	theme	insect	719:724	arg1	protein					743:749	the insect cell-expressed S protein	715:749	the insect cell-expressed S protein	715:749	In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type.					
33077685	10	14	theme	host	1310:1313	arg1	cells					1315:1319	distinct host cells	1301:1319	distinct host cells	1301:1319	This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.					
33077685	8	15	theme	subunits	1068:1075	arg1	sizes					1059:1063	different sizes	1049:1063	different sizes of subunits	1049:1075	Moreover, the N-glycan compositions were conserved among different sizes of subunits.					
33077685	2	16	from	level	334:338	arg1	N-glycosylation					298:312	the site-specific N-glycosylation	280:312	the site-specific N-glycosylation of S protein at the level of intact glycopeptides	280:362	Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.					
33077685	1	17	theme	glycoprotein	93:104	arg1	S					113:113	The glycoprotein spike (S)	89:114	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2)	89:191	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	1	17	theme	glycoprotein	93:104	arg1	determinant					198:208	a determinant	196:208	a determinant for viral invasion and host immune response	196:252	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	6	18	contain	contained	751:759	arg1	protein					743:749	the insect cell-expressed S protein	715:749	the insect cell-expressed S protein	715:749	In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type.					
33077685	6	18	contain	contained	751:759	arg2	N-glycans					764:772	38 N-glycans	761:772	38 N-glycans	761:772	In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type.					
33077685	6	18	contain	contained	751:759	arg1	contrast					705:712	contrast	705:712	contrast	705:712	In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type.					
33077685	6	18	contain	contained	751:759	arg2	protein					743:749	the insect cell-expressed S protein	715:749	the insect cell-expressed S protein	715:749	In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type.					
33077685	3	19	gly	N-glycosites	382:393	arg2	N-glycosites					382:393	All 22 potential N-glycosites	365:393	All 22 potential N-glycosites	365:393	All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-CoV-2 genome sequences.					
33077685	8	20	theme	N-glycan	1006:1013	arg1	compositions					1015:1026	the N-glycan compositions	1002:1026	the N-glycan compositions	1002:1026	Moreover, the N-glycan compositions were conserved among different sizes of subunits.					
33077685	1	21	theme	spike	106:110	arg1	S					113:113	The glycoprotein spike (S)	89:114	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2)	89:191	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	1	21	theme	spike	106:110	arg1	determinant					198:208	a determinant	196:208	a determinant for viral invasion and host immune response	196:252	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	10	22	theme	differential	1264:1275	arg1	patterns					1286:1293	the differential N-glycan patterns	1260:1293	the differential N-glycan patterns among distinct host cells	1260:1319	This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.					
33077685	0	23	theme	N-glycosylation	14:28	arg1	Characterization					30:45	Site-specific N-glycosylation Characterization	0:45	Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins.	0:87	Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins.					
33077685	5	24	theme	157	656:658	arg1	N-glycans					660:668	157 N-glycans	656:668	157 N-glycans	656:668	We identified masses that correspond to 157 N-glycans, primarily of the complex type.					
33077685	6	25	theme	high-mannose	793:804	arg1	type					806:809	the high-mannose type	789:809	the high-mannose type	789:809	In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type.					
33077685	2	26	theme	protein	319:325	arg1	N-glycosylation					298:312	the site-specific N-glycosylation	280:312	the site-specific N-glycosylation of S protein at the level of intact glycopeptides	280:362	Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.					
33077685	4	27	gly	glycosites	515:524	arg2	glycosites					515:524	The glycosites	511:524	The glycosites	511:524	The glycosites exhibited glycoform heterogeneity as expected for a human cell-expressed protein subunit.					
33077685	0	28	theme	Site-specific	0:12	arg1	Characterization					30:45	Site-specific N-glycosylation Characterization	0:45	Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins.	0:87	Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins.					
33077685	7	29	theme	insect	938:943	arg1	cells					945:949	human and insect cells	928:949	human and insect cells	928:949	Our results revealed that the glycan types were highly determined by the differential processing of N-glycans among human and insect cells, regardless of the glycosites' location.					
33077685	2	30	theme	S	317:317	arg1	protein					319:325	S protein	317:325	S protein	317:325	Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.					
33077685	7	31	theme	glycan	842:847	arg1	types					849:853	the glycan types	838:853	the glycan types	838:853	Our results revealed that the glycan types were highly determined by the differential processing of N-glycans among human and insect cells, regardless of the glycosites' location.					
33077685	10	32	theme	vaccines	1426:1433	arg1	development					1411:1421	the development	1407:1421	the development of vaccines and targeted drugs	1407:1452	This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.					
33077685	9	33	theme	protein	1109:1115	arg1	N-glycosylation					1117:1131	the S protein N-glycosylation	1103:1131	the S protein N-glycosylation	1103:1131	Our study indicates that the S protein N-glycosylation occurs regularly at each site, albeit the occupied N-glycans were diverse and heterogenous.					
33077685	3	34	theme	potential	372:380	arg1	N-glycosites					382:393	All 22 potential N-glycosites	365:393	All 22 potential N-glycosites	365:393	All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-CoV-2 genome sequences.					
33077685	4	35	theme	human	578:582	arg1	subunit					607:613	a human cell-expressed protein subunit	576:613	a human cell-expressed protein subunit	576:613	The glycosites exhibited glycoform heterogeneity as expected for a human cell-expressed protein subunit.					
33077685	2	36	theme	intact	343:348	arg1	glycopeptides					350:362	intact glycopeptides	343:362	intact glycopeptides	343:362	Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.					
33077685	9	37	theme	S	1107:1107	arg1	N-glycosylation					1117:1131	the S protein N-glycosylation	1103:1131	the S protein N-glycosylation	1103:1131	Our study indicates that the S protein N-glycosylation occurs regularly at each site, albeit the occupied N-glycans were diverse and heterogenous.					
33077685	0	38	theme	SARS-CoV-2	62:71	arg1	Proteins					79:86	Recombinant SARS-CoV-2 Spike Proteins	50:86	Recombinant SARS-CoV-2 Spike Proteins	50:86	Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins.					
33077685	0	39	theme	Recombinant	50:60	arg1	Proteins					79:86	Recombinant SARS-CoV-2 Spike Proteins	50:86	Recombinant SARS-CoV-2 Spike Proteins	50:86	Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins.					
33077685	4	40	theme	glycoform	536:544	arg1	heterogeneity					546:558	glycoform heterogeneity	536:558	glycoform heterogeneity	536:558	The glycosites exhibited glycoform heterogeneity as expected for a human cell-expressed protein subunit.					
33077685	2	41	gly	glycopeptides	350:362	arg2	glycopeptides					350:362	intact glycopeptides	343:362	intact glycopeptides	343:362	Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides.					
33077685	1	42	theme	viral	214:218	arg1	invasion					220:227	viral invasion	214:227	viral invasion	214:227	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	0	43	theme	Proteins	79:86	arg1	Characterization					30:45	Site-specific N-glycosylation Characterization	0:45	Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins.	0:87	Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins.					
33077685	5	44	theme	complex	688:694	arg1	type					696:699	the complex type	684:699	the complex type	684:699	We identified masses that correspond to 157 N-glycans, primarily of the complex type.					
33077685	7	45	gly	glycosites	970:979	arg2	glycosites					970:979	the glycosites'	966:980	the glycosites' location	966:989	Our results revealed that the glycan types were highly determined by the differential processing of N-glycans among human and insect cells, regardless of the glycosites' location.					
33077685	3	46	theme	SARS-CoV-2	482:491	arg1	sequences					500:508	the 753 SARS-CoV-2 genome sequences	474:508	the 753 SARS-CoV-2 genome sequences	474:508	All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-CoV-2 genome sequences.					
33077685	0	47	theme	Spike	73:77	arg1	Proteins					79:86	Recombinant SARS-CoV-2 Spike Proteins	50:86	Recombinant SARS-CoV-2 Spike Proteins	50:86	Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins.					
33077685	10	48	theme	infection	1355:1363	arg1	mechanism					1365:1373	the infection mechanism	1351:1373	the infection mechanism	1351:1373	This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.					
33077685	3	49	theme	genome	493:498	arg1	sequences					500:508	the 753 SARS-CoV-2 genome sequences	474:508	the 753 SARS-CoV-2 genome sequences	474:508	All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-CoV-2 genome sequences.					
33077685	7	50	theme	human	928:932	arg1	cells					945:949	human and insect cells	928:949	human and insect cells	928:949	Our results revealed that the glycan types were highly determined by the differential processing of N-glycans among human and insect cells, regardless of the glycosites' location.					
33077685	10	51	theme	drugs	1448:1452	arg1	development					1411:1421	the development	1407:1421	the development of vaccines and targeted drugs	1407:1452	This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.					
33077685	9	52	gly	N-glycosylation	1117:1131	arg2	site					1158:1161	each site	1153:1161	each site	1153:1161	Our study indicates that the S protein N-glycosylation occurs regularly at each site, albeit the occupied N-glycans were diverse and heterogenous.					
33077685	1	53	theme	severe	134:139	arg1	SARS-CoV-2					181:190	SARS-CoV-2	181:190	SARS-CoV-2	181:190	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	1	53	theme	severe	134:139	arg1	coronavirus					168:178	severe acute respiratory syndrome coronavirus	134:178	severe acute respiratory syndrome coronavirus (SARS-CoV-2)	134:191	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	7	54	theme	N-glycans	912:920	arg1	processing					898:907	the differential processing	881:907	the differential processing of N-glycans among human and insect cells	881:949	Our results revealed that the glycan types were highly determined by the differential processing of N-glycans among human and insect cells, regardless of the glycosites' location.					
33077685	10	55	theme	N-glycosylation	1230:1244	arg1	landscape					1246:1254	This N-glycosylation landscape	1225:1254	This N-glycosylation landscape	1225:1254	This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.					
33077685	10	56	theme	N-glycan	1277:1284	arg1	patterns					1286:1293	the differential N-glycan patterns	1260:1293	the differential N-glycan patterns among distinct host cells	1260:1319	This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.					
33077685	10	57	theme	positive	1389:1396	arg1	view					1398:1401	a positive view	1387:1401	a positive view for the development of vaccines and targeted drugs	1387:1452	This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.					
33077685	1	58	theme	acute	141:145	arg1	SARS-CoV-2					181:190	SARS-CoV-2	181:190	SARS-CoV-2	181:190	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	1	58	theme	acute	141:145	arg1	coronavirus					168:178	severe acute respiratory syndrome coronavirus	134:178	severe acute respiratory syndrome coronavirus (SARS-CoV-2)	134:191	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	1	59	from	S	113:113	arg1	surface					123:129	the surface	119:129	the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2)	119:191	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	1	60	theme	host	233:236	arg1	response					245:252	host immune response	233:252	host immune response	233:252	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	3	61	theme	S-protein	418:426	arg1	protomer					428:435	the S-protein protomer	414:435	the S-protein protomer	414:435	All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-CoV-2 genome sequences.					
33077685	6	62	theme	S	741:741	arg1	protein					743:749	the insect cell-expressed S protein	715:749	the insect cell-expressed S protein	715:749	In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type.					
33077685	1	63	theme	respiratory	147:157	arg1	SARS-CoV-2					181:190	SARS-CoV-2	181:190	SARS-CoV-2	181:190	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	1	63	theme	respiratory	147:157	arg1	coronavirus					168:178	severe acute respiratory syndrome coronavirus	134:178	severe acute respiratory syndrome coronavirus (SARS-CoV-2)	134:191	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
33077685	10	64	theme	distinct	1301:1308	arg1	cells					1315:1319	distinct host cells	1301:1319	distinct host cells	1301:1319	This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.					
33077685	1	65	theme	immune	238:243	arg1	response					245:252	host immune response	233:252	host immune response	233:252	The glycoprotein spike (S) on the surface of severe acute respiratory syndrome coronavirus (SARS-CoV-2) is a determinant for viral invasion and host immune response.					
31907375	7	0	located	observed	993:1000	arg2	changes					946:952	The prominent changes	932:952	The prominent changes in advanced disease stages	932:979	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	7	0	located	observed	993:1000	arg1	N-glycans					1022:1030	core fucosylated N-glycans	1005:1030	core fucosylated N-glycans with additional fucose (Fuc) residue/s	1005:1069	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	6	1	theme	cancerous	913:921	arg1	tissues					923:929	cancerous tissues	913:929	cancerous tissues	913:929	In addition, 42 new N-glycan compositions were detected in cancerous tissues.					
31907375	5	2	theme	paucimannose	636:647	arg1	glycans					666:672	paucimannose and high-mannose glycans	636:672	glycans	666:672	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	3	3	from	patients	454:461	arg1	specimens					436:444	fresh or defrosted tissue specimens	410:444	fresh or defrosted tissue specimens from 24 patients with LAC	410:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	6	4	located	detected	901:908	arg2	compositions					883:894	42 new N-glycan compositions	867:894	42 new N-glycan compositions	867:894	In addition, 42 new N-glycan compositions were detected in cancerous tissues.					
31907375	6	4	located	detected	901:908	arg1	addition					857:864	addition	857:864	addition	857:864	In addition, 42 new N-glycan compositions were detected in cancerous tissues.					
31907375	6	4	located	detected	901:908	arg1	tissues					923:929	cancerous tissues	913:929	cancerous tissues	913:929	In addition, 42 new N-glycan compositions were detected in cancerous tissues.					
31907375	7	5	theme	GlcNAc	1140:1145	arg1	units					1148:1152	non-galactosylated N-acetyl-glucosamine (GlcNAc) units	1099:1152	non-galactosylated N-acetyl-glucosamine (GlcNAc) units	1099:1152	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	0	6	theme	N-Glycan	0:7	arg1	profiling					9:17	N-Glycan profiling	0:17	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease	0:83	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease.					
31907375	1	7	theme	cancer	144:149	arg1	adenocarcinoma					91:104	Lung adenocarcinoma	86:104	Lung adenocarcinoma (LAC)	86:110	Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age.					
31907375	1	7	theme	cancer	144:149	arg1	form					131:134	the most common form	115:134	the most common form of lung cancer that increases in non-smokers at younger age	115:194	Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age.					
31907375	10	8	theme	detected	1566:1573	arg1	N-glycans					1575:1583	newly detected N-glycans	1560:1583	newly detected N-glycans	1560:1583	In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies.					
31907375	7	9	theme	N-acetyl-glucosamine	1118:1137	arg1	units					1148:1152	non-galactosylated N-acetyl-glucosamine (GlcNAc) units	1099:1152	non-galactosylated N-acetyl-glucosamine (GlcNAc) units	1099:1152	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	5	10	theme	mannose	683:689	arg1	residues					691:698	6-9 mannose residues	679:698	6-9 mannose residues	679:698	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	9	11	theme	noncancerous	1261:1272	arg1	tissues					1274:1280	noncancerous tissues	1261:1280	noncancerous tissues	1261:1280	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	5	12	from	decline	704:710	arg1	complex					730:736	the sialylated complex	715:736	the sialylated complex	715:736	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	2	13	theme	Altered	197:203	arg1	glycosylation					213:225	Altered protein glycosylation	197:225	Altered protein glycosylation	197:225	Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood.					
31907375	7	14	theme	additional	1037:1046	arg1	Fuc					1056:1058	Fuc	1056:1058	Fuc	1056:1058	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	7	14	theme	additional	1037:1046	arg1	fucose					1048:1053	additional fucose	1037:1053	additional fucose (Fuc) residue/s	1037:1069	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	1	15	theme	younger	184:190	arg1	age					192:194	younger age	184:194	younger age	184:194	Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age.					
31907375	5	16	with	decline	704:710	arg1	NeuAcGal2GlcNAc2Man3GlcNAc2Fuc					790:819	composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc	778:819	composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc	778:819	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	5	16	with	decline	704:710	arg1	residues					691:698	6-9 mannose residues	679:698	6-9 mannose residues	679:698	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	5	17	theme	high-mannose	653:664	arg1	glycans					666:672	paucimannose and high-mannose glycans	636:672	glycans	666:672	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	7	18	theme	core	1005:1008	arg1	N-glycans					1022:1030	core fucosylated N-glycans	1005:1030	core fucosylated N-glycans with additional fucose (Fuc) residue/s	1005:1069	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	11	19	from	approach	1757:1764	arg1	discrimination					1787:1800	rapid discrimination	1781:1800	rapid discrimination of cancerous from healthy lung tissues	1781:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	11	19	from	approach	1757:1764	arg1	tissues					1833:1839	healthy lung tissues	1820:1839	healthy lung tissues	1820:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	11	20	theme	rapid	1781:1785	arg1	discrimination					1787:1800	rapid discrimination	1781:1800	rapid discrimination of cancerous from healthy lung tissues	1781:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	5	21	theme	biantenary	738:747	arg1	glycan					766:771	biantenary core fucosylated glycan	738:771	biantenary core fucosylated glycan	738:771	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	9	22	theme	changes	1313:1319	arg1	detectable					1334:1343	detectable	1334:1343	detectable	1334:1343	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	9	22	theme	changes	1313:1319	arg1	number					1285:1290	a number	1283:1290	a number of these significant changes	1283:1319	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	1	23	theme	common	124:129	arg1	adenocarcinoma					91:104	Lung adenocarcinoma	86:104	Lung adenocarcinoma (LAC)	86:110	Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age.					
31907375	1	23	theme	common	124:129	arg1	form					131:134	the most common form	115:134	the most common form of lung cancer that increases in non-smokers at younger age	115:194	Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age.					
31907375	11	24	theme	lung	1828:1831	arg1	tissues					1833:1839	healthy lung tissues	1820:1839	healthy lung tissues	1820:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	5	25	theme	fucosylated	754:764	arg1	glycan					766:771	biantenary core fucosylated glycan	738:771	biantenary core fucosylated glycan	738:771	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	5	26	dep	glycans	666:672	arg1	glycan					766:771	biantenary core fucosylated glycan	738:771	biantenary core fucosylated glycan	738:771	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	7	27	theme	disease	966:972	arg1	stages					974:979	advanced disease stages	957:979	advanced disease stages	957:979	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	5	28	gly	fucosylated	754:764	arg1	glycan					766:771	biantenary core fucosylated glycan	738:771	biantenary core fucosylated glycan	738:771	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	5	29	theme	sialylated	719:728	arg1	complex					730:736	the sialylated complex	715:736	the sialylated complex	715:736	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	3	30	theme	MS	370:371	arg1	analysis					374:381	mass spectrometric (MS) analysis	350:381	mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC	350:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	5	31	theme	general	826:832	arg1	increase					624:631	The significant increase	608:631	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc	608:819	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	5	31	theme	general	826:832	arg1	features					834:841	general features	826:841	general features of tumors	826:851	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	9	32	theme	I.	1363:1364	arg1	Application					1366:1376	stage I. Application	1357:1376	stage I. Application of N-glycan data obtained from tissues	1357:1415	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	7	33	theme	prominent	936:944	arg1	changes					946:952	The prominent changes	932:952	The prominent changes in advanced disease stages	932:979	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	1	34	theme	Lung	86:89	arg1	form					131:134	the most common form	115:134	the most common form of lung cancer that increases in non-smokers at younger age	115:194	Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age.					
31907375	1	34	theme	Lung	86:89	arg1	LAC					107:109	LAC	107:109	LAC	107:109	Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age.					
31907375	1	34	theme	Lung	86:89	arg1	adenocarcinoma					91:104	Lung adenocarcinoma	86:104	Lung adenocarcinoma (LAC)	86:110	Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age.					
31907375	4	35	theme	cancerous	487:495	arg1	tissues					521:527	cancerous versus adjacent healthy tissues	487:527	cancerous versus adjacent healthy tissues	487:527	Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease.					
31907375	5	36	gly	sialylated	719:728	arg1	complex					730:736	the sialylated complex	715:736	the sialylated complex	715:736	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	6	37	theme	N-glycan	874:881	arg1	compositions					883:894	42 new N-glycan compositions	867:894	42 new N-glycan compositions	867:894	In addition, 42 new N-glycan compositions were detected in cancerous tissues.					
31907375	3	38	theme	spectrometric	355:367	arg1	analysis					374:381	mass spectrometric (MS) analysis	350:381	mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC	350:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	4	39	theme	adjacent	504:511	arg1	tissues					521:527	cancerous versus adjacent healthy tissues	487:527	cancerous versus adjacent healthy tissues	487:527	Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease.					
31907375	11	40	theme	presented	1747:1755	arg1	approach					1757:1764	the presented approach	1743:1764	the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues	1743:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	3	41	theme	N-glycans	386:394	arg1	analysis					374:381	mass spectrometric (MS) analysis	350:381	mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC	350:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	9	42	theme	small	1467:1471	arg1	biopsies					1479:1486	small sized biopsies	1467:1486	small sized biopsies obtained via bronchoscopy	1467:1512	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	9	43	theme	data	1390:1393	arg1	Application					1366:1376	stage I. Application	1357:1376	stage I. Application of N-glycan data obtained from tissues	1357:1415	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	0	44	theme	adenocarcinoma	27:40	arg1	profiling					9:17	N-Glycan profiling	0:17	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease	0:83	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease.					
31907375	0	45	theme	different	57:65	arg1	stages					67:72	different stages	57:72	different stages of disease	57:83	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease.					
31907375	3	46	theme	tissue	429:434	arg1	specimens					436:444	fresh or defrosted tissue specimens	410:444	fresh or defrosted tissue specimens from 24 patients with LAC	410:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	5	47	from	glycans	666:672	arg1	complex					730:736	the sialylated complex	715:736	the sialylated complex	715:736	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	9	48	theme	sized	1473:1477	arg1	biopsies					1479:1486	small sized biopsies	1467:1486	small sized biopsies obtained via bronchoscopy	1467:1512	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	10	49	theme	N-glycans	1575:1583	arg1	data					1552:1555	data	1552:1555	data	1552:1555	In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies.					
31907375	10	49	theme	N-glycans	1575:1583	arg1	alterations					1536:1546	alterations	1536:1546	alterations	1536:1546	In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies.					
31907375	5	50	theme	core	749:752	arg1	glycan					766:771	biantenary core fucosylated glycan	738:771	biantenary core fucosylated glycan	738:771	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	5	51	theme	significant	612:622	arg1	increase					624:631	The significant increase	608:631	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc	608:819	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	5	51	theme	significant	612:622	arg1	features					834:841	general features	826:841	general features of tumors	826:851	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	8	52	theme	monosaccharide	1169:1182	arg1	types					1184:1188	these monosaccharide types	1163:1188	these monosaccharide types	1163:1188	Both of these monosaccharide types were linked preferably on the 6-antenna.					
31907375	4	53	theme	tissues	521:527	arg1	Comparison					473:482	Comparison	473:482	Comparison of cancerous versus adjacent healthy tissues	473:527	Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease.					
31907375	3	54	with	patients	454:461	arg1	LAC					468:470	LAC	468:470	LAC	468:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	2	55	theme	malignancy	254:263	arg1	hallmarks					241:249	the hallmarks	237:249	the hallmarks of malignancy	237:263	Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood.					
31907375	10	56	from	research	1655:1662	arg1	therapies					1681:1689	more tailored therapies	1667:1689	more tailored therapies	1667:1689	In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies.					
31907375	3	57	attach	released	396:403	arg1	specimens					436:444	fresh or defrosted tissue specimens	410:444	fresh or defrosted tissue specimens from 24 patients with LAC	410:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	3	57	attach	released	396:403	arg2	N-glycans					386:394	N-glycans	386:394	N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC	386:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	4	58	theme	substantial	538:548	arg1	differences					550:560	substantial differences	538:560	substantial differences in N-glycan profiles associated with disease	538:605	Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease.					
31907375	2	59	theme	hallmarks	241:249	arg1	one					230:232	one	230:232	one	230:232	Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood.					
31907375	2	59	theme	hallmarks	241:249	arg1	hallmarks					241:249	the hallmarks	237:249	the hallmarks of malignancy	237:263	Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood.					
31907375	1	60	theme	lung	139:142	arg1	cancer					144:149	lung cancer	139:149	lung cancer	139:149	Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age.					
31907375	7	61	theme	non-galactosylated	1099:1116	arg1	units					1148:1152	non-galactosylated N-acetyl-glucosamine (GlcNAc) units	1099:1152	non-galactosylated N-acetyl-glucosamine (GlcNAc) units	1099:1152	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	9	62	theme	biopsies	1479:1486	arg1	evaluation					1453:1462	evaluation	1453:1462	evaluation of small sized biopsies obtained via bronchoscopy	1453:1512	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	8	63	attach	linked	1195:1200	arg2	Both					1155:1158	Both	1155:1158	Both	1155:1158	Both of these monosaccharide types were linked preferably on the 6-antenna.					
31907375	8	63	attach	linked	1195:1200	arg3	6-antenna					1220:1228	the 6-antenna	1216:1228	the 6-antenna	1216:1228	Both of these monosaccharide types were linked preferably on the 6-antenna.					
31907375	8	63	attach	linked	1195:1200	arg2	types					1184:1188	these monosaccharide types	1163:1188	these monosaccharide types	1163:1188	Both of these monosaccharide types were linked preferably on the 6-antenna.					
31907375	5	64	theme	tumors	846:851	arg1	increase					624:631	The significant increase	608:631	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc	608:819	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	5	64	theme	tumors	846:851	arg1	features					834:841	general features	826:841	general features of tumors	826:851	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	2	65	theme	protein	205:211	arg1	glycosylation					213:225	Altered protein glycosylation	197:225	Altered protein glycosylation	197:225	Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood.					
31907375	11	66	from	tissues	1833:1839	arg1	discrimination					1787:1800	rapid discrimination	1781:1800	rapid discrimination of cancerous from healthy lung tissues	1781:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	11	66	from	tissues	1833:1839	arg1	approach					1757:1764	the presented approach	1743:1764	the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues	1743:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	0	67	from	stages	67:72	arg1	patients					45:52	patients	45:52	patients at different stages of disease	45:83	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease.					
31907375	0	67	from	stages	67:72	arg1	profiling					9:17	N-Glycan profiling	0:17	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease	0:83	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease.					
31907375	10	68	gly	glycosylation	1612:1624	arg1	LAC					1629:1631	LAC	1629:1631	LAC	1629:1631	In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies.					
31907375	11	69	from	effectiveness	1726:1738	arg1	discrimination					1787:1800	rapid discrimination	1781:1800	rapid discrimination of cancerous from healthy lung tissues	1781:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	7	70	theme	fucose	1048:1053	arg1	residue/s					1061:1069	additional fucose (Fuc) residue/s	1037:1069	additional fucose (Fuc) residue/s	1037:1069	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	11	71	from	discrimination	1787:1800	arg1	effectiveness					1726:1738	effectiveness	1726:1738	effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues	1726:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	7	72	from	changes	946:952	arg1	stages					974:979	advanced disease stages	957:979	advanced disease stages	957:979	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	11	73	theme	cancerous	1805:1813	arg1	discrimination					1787:1800	rapid discrimination	1781:1800	rapid discrimination of cancerous from healthy lung tissues	1781:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	9	74	theme	significant	1301:1311	arg1	changes					1313:1319	these significant changes	1295:1319	these significant changes	1295:1319	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	7	75	theme	fucosylated	1010:1020	arg1	N-glycans					1022:1030	core fucosylated N-glycans	1005:1030	core fucosylated N-glycans with additional fucose (Fuc) residue/s	1005:1069	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	0	76	theme	disease	77:83	arg1	stages					67:72	different stages	57:72	different stages of disease	57:83	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease.					
31907375	7	77	with	N-glycans	1022:1030	arg1	residue/s					1061:1069	additional fucose (Fuc) residue/s	1037:1069	additional fucose (Fuc) residue/s	1037:1069	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	0	78	from	profiling	9:17	arg1	patients					45:52	patients	45:52	patients at different stages of disease	45:83	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease.					
31907375	0	78	from	profiling	9:17	arg1	stages					67:72	different stages	57:72	different stages of disease	57:83	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease.					
31907375	4	79	from	differences	550:560	arg1	profiles					574:581	N-glycan profiles	565:581	N-glycan profiles associated with disease	565:605	Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease.					
31907375	2	80	from	role	270:273	arg1	progression					285:295	cancer progression	278:295	cancer progression	278:295	Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood.					
31907375	2	81	theme	cancer	278:283	arg1	progression					285:295	cancer progression	278:295	cancer progression	278:295	Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood.					
31907375	5	82	with	glycans	666:672	arg1	NeuAcGal2GlcNAc2Man3GlcNAc2Fuc					790:819	composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc	778:819	composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc	778:819	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	5	82	with	glycans	666:672	arg1	residues					691:698	6-9 mannose residues	679:698	6-9 mannose residues	679:698	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	11	83	theme	healthy	1820:1826	arg1	tissues					1833:1839	healthy lung tissues	1820:1839	healthy lung tissues	1820:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	9	84	theme	stage	1357:1361	arg1	Application					1366:1376	stage I. Application	1357:1376	stage I. Application of N-glycan data obtained from tissues	1357:1415	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	7	85	theme	advanced	957:964	arg1	stages					974:979	advanced disease stages	957:979	advanced disease stages	957:979	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	7	86	gly	fucosylated	1010:1020	arg1	N-glycans					1022:1030	core fucosylated N-glycans	1005:1030	core fucosylated N-glycans with additional fucose (Fuc) residue/s	1005:1069	The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units.					
31907375	10	87	theme	tailored	1672:1679	arg1	therapies					1681:1689	more tailored therapies	1667:1689	more tailored therapies	1667:1689	In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies.					
31907375	0	88	theme	lung	22:25	arg1	adenocarcinoma					27:40	lung adenocarcinoma	22:40	lung adenocarcinoma	22:40	N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease.					
31907375	4	89	theme	healthy	513:519	arg1	tissues					521:527	cancerous versus adjacent healthy tissues	487:527	cancerous versus adjacent healthy tissues	487:527	Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease.					
31907375	10	90	theme	observed	1527:1534	arg1	alterations					1536:1546	alterations	1536:1546	alterations	1536:1546	In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies.					
31907375	3	91	theme	fresh	410:414	arg1	specimens					436:444	fresh or defrosted tissue specimens	410:444	fresh or defrosted tissue specimens from 24 patients with LAC	410:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	9	92	theme	N-glycan	1381:1388	arg1	data					1390:1393	N-glycan data	1381:1393	N-glycan data obtained from tissues	1381:1415	Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy.					
31907375	6	93	theme	new	870:872	arg1	compositions					883:894	42 new N-glycan compositions	867:894	42 new N-glycan compositions	867:894	In addition, 42 new N-glycan compositions were detected in cancerous tissues.					
31907375	5	94	theme	composition	778:788	arg1	NeuAcGal2GlcNAc2Man3GlcNAc2Fuc					790:819	composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc	778:819	composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc	778:819	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	3	95	theme	mass	350:353	arg1	analysis					374:381	mass spectrometric (MS) analysis	350:381	mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC	350:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	5	96	from	increase	624:631	arg1	glycans					666:672	paucimannose and high-mannose glycans	636:672	glycans	666:672	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	5	96	from	increase	624:631	arg1	decline					704:710	decline	704:710	decline in the sialylated complex	704:736	The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors.					
31907375	4	97	theme	N-glycan	565:572	arg1	profiles					574:581	N-glycan profiles	565:581	N-glycan profiles associated with disease	565:605	Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease.					
31907375	11	98	theme	approach	1757:1764	arg1	effectiveness					1726:1738	effectiveness	1726:1738	effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues	1726:1839	Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.					
31907375	3	99	theme	defrosted	419:427	arg1	specimens					436:444	fresh or defrosted tissue specimens	410:444	fresh or defrosted tissue specimens from 24 patients with LAC	410:470	In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC.					
31907375	10	100	from	glycosylation	1612:1624	arg1	LAC					1629:1631	LAC	1629:1631	LAC	1629:1631	In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies.					
33871988	4	0	dep	enrichment	846:855	arg1	handles					857:863	handles	857:863	handles	857:863	Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles.					
33871988	6	1	gly	glycopeptides	1119:1131	arg2	glycopeptides					1119:1131	new GalNAc analogue glycopeptides	1099:1131	new GalNAc analogue glycopeptides	1099:1131	In this work, we built on this discovery by generating a series of new GalNAc analogue glycopeptides.					
33871988	6	2	theme	GalNAc	1103:1108	arg1	glycopeptides					1119:1131	new GalNAc analogue glycopeptides	1099:1131	new GalNAc analogue glycopeptides	1099:1131	In this work, we built on this discovery by generating a series of new GalNAc analogue glycopeptides.					
33871988	0	3	theme	Glycoproteomic	75:88	arg1	Analyses					90:97	Glycoproteomic Analyses	75:97	Glycoproteomic Analyses	75:97	Benefits of Chemical Sugar Modifications Introduced by Click Chemistry for Glycoproteomic Analyses.					
33871988	7	4	theme	bioorthogonal	1254:1266	arg1	reagents					1277:1284	bioorthogonal reporter reagents	1254:1284	bioorthogonal reporter reagents	1254:1284	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	4	5	theme	bioorthogonal	799:811	arg1	derivatization					813:826	bioorthogonal derivatization	799:826	bioorthogonal derivatization with biotin-based enrichment handles	799:863	Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles.					
33871988	5	6	contain	have	945:948	arg1	modifications					909:921	the chemical modifications	896:921	the chemical modifications used in these studies	896:943	In doing so, we realized that the chemical modifications used in these studies have additional benefits that allow for improved analysis by tandem mass spectrometry.					
33871988	5	6	contain	have	945:948	arg2	benefits					961:968	additional benefits	950:968	additional benefits that allow for improved analysis by tandem mass spectrometry	950:1029	In doing so, we realized that the chemical modifications used in these studies have additional benefits that allow for improved analysis by tandem mass spectrometry.					
33871988	3	7	theme	mass	413:416	arg1	spectrometry					418:429	mass spectrometry	413:429	mass spectrometry	413:429	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	5	8	theme	mass	1013:1016	arg1	spectrometry					1018:1029	tandem mass spectrometry	1006:1029	tandem mass spectrometry	1006:1029	In doing so, we realized that the chemical modifications used in these studies have additional benefits that allow for improved analysis by tandem mass spectrometry.					
33871988	4	9	with	derivatization	813:826	arg1	enrichment					846:855	biotin-based enrichment handles	833:863	biotin-based enrichment handles	833:863	Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles.					
33871988	8	10	gly	glycopeptide	1331:1342	arg2	glycopeptide					1331:1342	glycopeptide profiling	1331:1352	glycopeptide profiling	1331:1352	Our data indicate that chemical methods for glycopeptide profiling offer opportunities to optimize attributes such as increased charge state, higher charge density, and predictable fragmentation behavior.					
33871988	3	11	theme	O-glycopeptides	394:408	arg1	analysis					375:382	analysis	375:382	analysis of intact O-glycopeptides by mass spectrometry	375:429	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	8	12	theme	chemical	1310:1317	arg1	methods					1319:1325	chemical methods	1310:1325	chemical methods for glycopeptide profiling	1310:1352	Our data indicate that chemical methods for glycopeptide profiling offer opportunities to optimize attributes such as increased charge state, higher charge density, and predictable fragmentation behavior.					
33871988	8	13	theme	charge	1415:1420	arg1	state					1422:1426	increased charge state	1405:1426	increased charge state	1405:1426	Our data indicate that chemical methods for glycopeptide profiling offer opportunities to optimize attributes such as increased charge state, higher charge density, and predictable fragmentation behavior.					
33871988	2	14	theme	analytical	330:339	arg1	tools					341:345	the right analytical tools	320:345	the right analytical tools	320:345	Despite mediating many physiological processes, O-glycosylation remains understudied compared to other modifications, simply because the right analytical tools are lacking.					
33871988	8	15	theme	glycopeptide	1331:1342	arg1	profiling					1344:1352	glycopeptide profiling	1331:1352	glycopeptide profiling	1331:1352	Our data indicate that chemical methods for glycopeptide profiling offer opportunities to optimize attributes such as increased charge state, higher charge density, and predictable fragmentation behavior.					
33871988	5	16	theme	chemical	900:907	arg1	modifications					909:921	the chemical modifications	896:921	the chemical modifications used in these studies	896:943	In doing so, we realized that the chemical modifications used in these studies have additional benefits that allow for improved analysis by tandem mass spectrometry.					
33871988	7	17	theme	signature	1227:1235	arg1	glycopeptides					1203:1215	these modified glycopeptides	1188:1215	these modified glycopeptides	1188:1215	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	7	17	theme	signature	1227:1235	arg1	residues					1237:1244	their signature residues	1221:1244	their signature residues left by bioorthogonal reporter reagents	1221:1284	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	7	18	theme	modified	1194:1201	arg1	glycopeptides					1203:1215	these modified glycopeptides	1188:1215	these modified glycopeptides	1188:1215	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	2	19	theme	right	324:328	arg1	tools					341:345	the right analytical tools	320:345	the right analytical tools	320:345	Despite mediating many physiological processes, O-glycosylation remains understudied compared to other modifications, simply because the right analytical tools are lacking.					
33871988	5	20	theme	improved	985:992	arg1	analysis					994:1001	improved analysis	985:1001	improved analysis by tandem mass spectrometry	985:1029	In doing so, we realized that the chemical modifications used in these studies have additional benefits that allow for improved analysis by tandem mass spectrometry.					
33871988	7	21	dep	residues	1237:1244	arg1	left					1246:1249	left	1246:1249	left	1246:1249	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	7	22	theme	glycopeptides	1203:1215	arg1	glycopeptides					1203:1215	these modified glycopeptides	1188:1215	these modified glycopeptides	1188:1215	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	7	22	theme	glycopeptides	1203:1215	arg1	residues					1237:1244	their signature residues	1221:1244	their signature residues left by bioorthogonal reporter reagents	1221:1284	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	7	22	theme	glycopeptides	1203:1215	arg1	signatures					1174:1183	the mass spectrometric signatures	1151:1183	the mass spectrometric signatures of these modified glycopeptides	1151:1215	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	4	23	theme	mucin-type	772:781	arg1	O-glycans					783:791	mucin-type O-glycans	772:791	mucin-type O-glycans	772:791	Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles.					
33871988	3	24	theme	several	450:456	arg1	reasons					458:464	several reasons	450:464	several reasons	450:464	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	1	25	theme	Mucin-type	100:109	arg1	O-glycosylation					111:125	Mucin-type O-glycosylation	100:125	Mucin-type O-glycosylation	100:125	Mucin-type O-glycosylation is among the most complex post-translational modifications.					
33871988	3	26	theme	consensus	491:499	arg1	motif					501:505	a consensus motif	489:505	a consensus motif	489:505	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	0	27	theme	Sugar	21:25	arg1	Modifications					27:39	Chemical Sugar Modifications	12:39	Chemical Sugar Modifications	12:39	Benefits of Chemical Sugar Modifications Introduced by Click Chemistry for Glycoproteomic Analyses.					
33871988	8	28	theme	predictable	1456:1466	arg1	behavior					1482:1489	predictable fragmentation behavior	1456:1489	predictable fragmentation behavior	1456:1489	Our data indicate that chemical methods for glycopeptide profiling offer opportunities to optimize attributes such as increased charge state, higher charge density, and predictable fragmentation behavior.					
33871988	3	29	theme	low	527:529	arg1	density					538:544	low charge density	527:544	low charge density which impairs ETD fragmentation	527:576	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	0	30	theme	Chemical	12:19	arg1	Modifications					27:39	Chemical Sugar Modifications	12:39	Chemical Sugar Modifications	12:39	Benefits of Chemical Sugar Modifications Introduced by Click Chemistry for Glycoproteomic Analyses.					
33871988	3	31	theme	glycan	587:592	arg1	unpredictable					632:644	unpredictable	632:644	unpredictable	632:644	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	3	31	theme	glycan	587:592	arg1	structures					594:603	the glycan structures	583:603	the glycan structures modifying the peptides	583:626	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	3	32	contain	have	522:525	arg2	density					538:544	low charge density	527:544	low charge density which impairs ETD fragmentation	527:576	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	3	32	contain	have	522:525	arg1	glycopeptides					508:520	glycopeptides	508:520	glycopeptides	508:520	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	3	33	theme	charge	531:536	arg1	density					538:544	low charge density	527:544	low charge density which impairs ETD fragmentation	527:576	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	8	34	theme	charge	1436:1441	arg1	density					1443:1449	higher charge density	1429:1449	higher charge density	1429:1449	Our data indicate that chemical methods for glycopeptide profiling offer opportunities to optimize attributes such as increased charge state, higher charge density, and predictable fragmentation behavior.					
33871988	2	35	theme	physiological	210:222	arg1	processes					224:232	many physiological processes	205:232	many physiological processes	205:232	Despite mediating many physiological processes, O-glycosylation remains understudied compared to other modifications, simply because the right analytical tools are lacking.					
33871988	7	36	gly	glycopeptides	1203:1215	arg2	glycopeptides					1203:1215	these modified glycopeptides	1188:1215	these modified glycopeptides	1188:1215	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	7	37	theme	reporter	1268:1275	arg1	reagents					1277:1284	bioorthogonal reporter reagents	1254:1284	bioorthogonal reporter reagents	1254:1284	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	0	38	theme	Modifications	27:39	arg1	Benefits					0:7	Benefits	0:7	Benefits of Chemical Sugar Modifications	0:39	Benefits of Chemical Sugar Modifications Introduced by Click Chemistry for Glycoproteomic Analyses.					
33871988	3	39	gly	O-glycopeptides	394:408	arg2	O-glycopeptides					394:408	intact O-glycopeptides	387:408	intact O-glycopeptides	387:408	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	2	40	theme	many	205:208	arg1	processes					224:232	many physiological processes	205:232	many physiological processes	205:232	Despite mediating many physiological processes, O-glycosylation remains understudied compared to other modifications, simply because the right analytical tools are lacking.					
33871988	2	41	theme	other	284:288	arg1	modifications					290:302	other modifications	284:302	other modifications	284:302	Despite mediating many physiological processes, O-glycosylation remains understudied compared to other modifications, simply because the right analytical tools are lacking.					
33871988	4	42	theme	selective	729:737	arg1	tracking					739:746	selective tracking	729:746	selective tracking	729:746	Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles.					
33871988	0	43	theme	Click	55:59	arg1	Chemistry					61:69	Click Chemistry	55:69	Click Chemistry for Glycoproteomic Analyses	55:97	Benefits of Chemical Sugar Modifications Introduced by Click Chemistry for Glycoproteomic Analyses.					
33871988	5	44	theme	tandem	1006:1011	arg1	spectrometry					1018:1029	tandem mass spectrometry	1006:1029	tandem mass spectrometry	1006:1029	In doing so, we realized that the chemical modifications used in these studies have additional benefits that allow for improved analysis by tandem mass spectrometry.					
33871988	7	45	theme	mass	1155:1158	arg1	signatures					1174:1183	the mass spectrometric signatures	1151:1183	the mass spectrometric signatures of these modified glycopeptides	1151:1215	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	4	46	theme	biotin-based	833:844	arg1	enrichment					846:855	biotin-based enrichment handles	833:863	biotin-based enrichment handles	833:863	Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles.					
33871988	7	47	theme	spectrometric	1160:1172	arg1	signatures					1174:1183	the mass spectrometric signatures	1151:1183	the mass spectrometric signatures of these modified glycopeptides	1151:1215	We characterized the mass spectrometric signatures of these modified glycopeptides and their signature residues left by bioorthogonal reporter reagents.					
33871988	3	48	gly	glycopeptides	508:520	arg2	glycopeptides					508:520	glycopeptides	508:520	glycopeptides	508:520	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	3	49	theme	ETD	560:562	arg1	fragmentation					564:576	ETD fragmentation	560:576	ETD fragmentation	560:576	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	8	50	theme	higher	1429:1434	arg1	density					1443:1449	higher charge density	1429:1449	higher charge density	1429:1449	Our data indicate that chemical methods for glycopeptide profiling offer opportunities to optimize attributes such as increased charge state, higher charge density, and predictable fragmentation behavior.					
33871988	8	51	theme	increased	1405:1413	arg1	state					1422:1426	increased charge state	1405:1426	increased charge state	1405:1426	Our data indicate that chemical methods for glycopeptide profiling offer opportunities to optimize attributes such as increased charge state, higher charge density, and predictable fragmentation behavior.					
33871988	1	52	theme	most	140:143	arg1	modifications					172:184	the most complex post-translational modifications	136:184	the most complex post-translational modifications	136:184	Mucin-type O-glycosylation is among the most complex post-translational modifications.					
33871988	4	53	theme	modified	682:689	arg1	analogues					706:714	chemically modified monosaccharide analogues	671:714	chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles	671:863	Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles.					
33871988	8	54	theme	fragmentation	1468:1480	arg1	behavior					1482:1489	predictable fragmentation behavior	1456:1489	predictable fragmentation behavior	1456:1489	Our data indicate that chemical methods for glycopeptide profiling offer opportunities to optimize attributes such as increased charge state, higher charge density, and predictable fragmentation behavior.					
33871988	1	55	theme	complex	145:151	arg1	modifications					172:184	the most complex post-translational modifications	136:184	the most complex post-translational modifications	136:184	Mucin-type O-glycosylation is among the most complex post-translational modifications.					
33871988	5	56	theme	additional	950:959	arg1	benefits					961:968	additional benefits	950:968	additional benefits that allow for improved analysis by tandem mass spectrometry	950:1029	In doing so, we realized that the chemical modifications used in these studies have additional benefits that allow for improved analysis by tandem mass spectrometry.					
33871988	1	57	theme	post-translational	153:170	arg1	modifications					172:184	the most complex post-translational modifications	136:184	the most complex post-translational modifications	136:184	Mucin-type O-glycosylation is among the most complex post-translational modifications.					
33871988	6	58	theme	new	1099:1101	arg1	glycopeptides					1119:1131	new GalNAc analogue glycopeptides	1099:1131	new GalNAc analogue glycopeptides	1099:1131	In this work, we built on this discovery by generating a series of new GalNAc analogue glycopeptides.					
33871988	6	59	theme	glycopeptides	1119:1131	arg1	series					1089:1094	a series	1087:1094	a series of new GalNAc analogue glycopeptides	1087:1131	In this work, we built on this discovery by generating a series of new GalNAc analogue glycopeptides.					
33871988	3	60	theme	intact	387:392	arg1	O-glycopeptides					394:408	intact O-glycopeptides	387:408	intact O-glycopeptides	387:408	In particular, analysis of intact O-glycopeptides by mass spectrometry is challenging for several reasons; O-glycosylation lacks a consensus motif, glycopeptides have low charge density which impairs ETD fragmentation, and the glycan structures modifying the peptides are unpredictable.					
33871988	6	61	theme	analogue	1110:1117	arg1	glycopeptides					1119:1131	new GalNAc analogue glycopeptides	1099:1131	new GalNAc analogue glycopeptides	1099:1131	In this work, we built on this discovery by generating a series of new GalNAc analogue glycopeptides.					
33871988	4	62	theme	O-glycans	783:791	arg1	characterization					752:767	characterization	752:767	characterization	752:767	Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles.					
33871988	4	62	theme	O-glycans	783:791	arg1	tracking					739:746	selective tracking	729:746	selective tracking	729:746	Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles.					
33871988	4	63	theme	monosaccharide	691:704	arg1	analogues					706:714	chemically modified monosaccharide analogues	671:714	chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles	671:863	Recently, we introduced chemically modified monosaccharide analogues that allowed selective tracking and characterization of mucin-type O-glycans after bioorthogonal derivatization with biotin-based enrichment handles.					
32178593	2	0	theme	host	450:453	arg1	adhesion					460:467	host cell adhesion	450:467	host cell adhesion	450:467	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	3	1	theme	defence	659:665	arg1	system					667:672	the defence system	655:672	the host the defence system	646:672	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	2	2	theme	COVID-19	334:341	arg1	glycoprotein					349:360	COVID-19 spike glycoprotein	334:360	COVID-19 spike glycoprotein	334:360	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	0	3	theme	structure	57:65	arg1	prediction					67:76	structure prediction	57:76	structure prediction of spike glycoprotein	57:98	Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26.					
32178593	0	4	with	prediction	67:76	arg1	CD26					131:134	human CD26	125:134	human CD26	125:134	Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26.					
32178593	1	5	theme	recent	141:146	arg1	outbreak					148:155	The recent outbreak	137:155	The recent outbreak of pneumonia-causing COVID-19 in China	137:194	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	1	5	theme	recent	141:146	arg1	issue					230:234	an urgent global public health issue	199:234	an urgent global public health issue with an increase in mortality and morbidity	199:278	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	0	6	theme	spike	81:85	arg1	glycoprotein					87:98	spike glycoprotein	81:98	spike glycoprotein	81:98	Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26.					
32178593	4	7	theme	key	825:827	arg1	factor					846:851	a key immunoregulatory factor	823:851	a key immunoregulatory factor for hijacking and virulence	823:879	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	4	7	theme	key	825:827	arg1	CD26					817:820	the human CD26	807:820	the human CD26	807:820	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	5	8	theme	therapeutics	977:988	arg1	development					958:968	the development	954:968	the development of new therapeutics	954:988	These findings accentuate the unique features of COVID-19 and assist in the development of new therapeutics.					
32178593	4	9	theme	key	718:720	arg1	finding					722:728	the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence	714:879	the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence	714:879	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	3	10	theme	O-linked	504:511	arg1	glycosylation					513:525	O-linked glycosylation	504:525	O-linked glycosylation	504:525	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	2	11	from	structure	321:329	arg1	conformation					415:426	both closed (ligand-free) and open (ligand-bound) conformation	365:426	conformation	415:426	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	2	11	from	structure	321:329	arg1	ligand-free					378:388	ligand-free	378:388	ligand-free	378:388	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	5	12	theme	COVID-19	931:938	arg1	features					919:926	the unique features	908:926	the unique features of COVID-19	908:938	These findings accentuate the unique features of COVID-19 and assist in the development of new therapeutics.					
32178593	3	13	dep	N-	497:498	arg1	sites					527:531	sites	527:531	sites	527:531	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	0	14	theme	glycoprotein	87:98	arg1	prediction					67:76	structure prediction	57:76	structure prediction of spike glycoprotein	57:98	Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26.					
32178593	0	14	theme	glycoprotein	87:98	arg1	interaction					108:118	its interaction	104:118	its interaction with human CD26	104:134	Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26.					
32178593	3	15	theme	unique	490:495	arg1	N-					497:498	the unique N-	486:498	the unique N-	486:498	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	0	16	theme	WuHan	9:13	arg1	coronavirus					26:36	WuHan (COVID-19) coronavirus	9:36	WuHan (COVID-19) coronavirus	9:36	Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26.					
32178593	4	17	theme	human	811:815	arg1	factor					846:851	a key immunoregulatory factor	823:851	a key immunoregulatory factor for hijacking and virulence	823:879	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	4	17	theme	human	811:815	arg1	CD26					817:820	the human CD26	807:820	the human CD26	807:820	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	1	18	theme	pneumonia-causing	160:176	arg1	COVID-19					178:185	pneumonia-causing COVID-19	160:185	pneumonia-causing COVID-19	160:185	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	1	19	theme	COVID-19	178:185	arg1	outbreak					148:155	The recent outbreak	137:155	The recent outbreak of pneumonia-causing COVID-19 in China	137:194	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	1	19	theme	COVID-19	178:185	arg1	issue					230:234	an urgent global public health issue	199:234	an urgent global public health issue with an increase in mortality and morbidity	199:278	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	2	20	theme	ligand-bound	401:412	arg1	conformation					415:426	both closed (ligand-free) and open (ligand-bound) conformation	365:426	conformation	415:426	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	3	21	dep	host	650:653	arg1	system					667:672	the defence system	655:672	the host the defence system	646:672	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	4	22	theme	glycoprotein	767:778	arg1	domain					742:747	the S1 domain	735:747	the S1 domain of COVID-19 spike glycoprotein	735:778	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	4	22	theme	glycoprotein	767:778	arg1	glycoprotein					767:778	COVID-19 spike glycoprotein	752:778	COVID-19 spike glycoprotein	752:778	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	0	23	gly	glycoprotein	87:98	arg1	glycoprotein					87:98	spike glycoprotein	81:98	spike glycoprotein	81:98	Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26.					
32178593	2	24	theme	open	395:398	arg1	conformation					415:426	both closed (ligand-free) and open (ligand-bound) conformation	365:426	conformation	415:426	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	2	25	gly	glycoprotein	349:360	arg1	glycoprotein					349:360	COVID-19 spike glycoprotein	334:360	COVID-19 spike glycoprotein	334:360	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	5	26	theme	unique	912:917	arg1	features					919:926	the unique features	908:926	the unique features of COVID-19	908:938	These findings accentuate the unique features of COVID-19 and assist in the development of new therapeutics.					
32178593	2	27	theme	glycoprotein	349:360	arg1	structure					321:329	our modelled homo-trimer structure	296:329	our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion	296:467	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	0	28	theme	human	125:129	arg1	CD26					131:134	human CD26	125:134	human CD26	125:134	Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26.					
32178593	4	29	theme	spike	761:765	arg1	glycoprotein					767:778	COVID-19 spike glycoprotein	752:778	COVID-19 spike glycoprotein	752:778	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	3	30	gly	glycoprotein	542:553	arg1	glycoprotein					542:553	spike glycoprotein	536:553	spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system	536:672	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	3	31	from	host	650:653	arg1	shielding					604:612	shielding	604:612	shielding	604:612	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	3	31	from	host	650:653	arg1	camouflage					618:627	camouflage	618:627	camouflage	618:627	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	4	32	gly	glycoprotein	767:778	arg1	glycoprotein					767:778	COVID-19 spike glycoprotein	752:778	COVID-19 spike glycoprotein	752:778	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	3	33	theme	spike	536:540	arg1	glycoprotein					542:553	spike glycoprotein	536:553	spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system	536:672	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	4	34	theme	immunoregulatory	829:844	arg1	factor					846:851	a key immunoregulatory factor	823:851	a key immunoregulatory factor for hijacking and virulence	823:879	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	4	34	theme	immunoregulatory	829:844	arg1	CD26					817:820	the human CD26	807:820	the human CD26	807:820	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	3	35	theme	COVID-19	632:639	arg1	shielding					604:612	shielding	604:612	shielding	604:612	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	3	35	theme	COVID-19	632:639	arg1	camouflage					618:627	camouflage	618:627	camouflage	618:627	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	3	36	theme	glycoprotein	542:553	arg1	N-					497:498	the unique N-	486:498	the unique N-	486:498	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	3	36	theme	glycoprotein	542:553	arg1	glycosylation					513:525	O-linked glycosylation	504:525	O-linked glycosylation	504:525	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	1	37	with	issue	230:234	arg1	increase					244:251	an increase	241:251	an increase in mortality and morbidity	241:278	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	2	38	theme	homo-trimer	309:319	arg1	structure					321:329	our modelled homo-trimer structure	296:329	our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion	296:467	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	5	39	theme	new	973:975	arg1	therapeutics					977:988	new therapeutics	973:988	new therapeutics	973:988	These findings accentuate the unique features of COVID-19 and assist in the development of new therapeutics.					
32178593	4	40	theme	S1	739:740	arg1	domain					742:747	the S1 domain	735:747	the S1 domain of COVID-19 spike glycoprotein	735:778	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	4	40	theme	S1	739:740	arg1	glycoprotein					767:778	COVID-19 spike glycoprotein	752:778	COVID-19 spike glycoprotein	752:778	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	4	41	theme	COVID-19	752:759	arg1	glycoprotein					767:778	COVID-19 spike glycoprotein	752:778	COVID-19 spike glycoprotein	752:778	Furthermore, our study also highlights the key finding that the S1 domain of COVID-19 spike glycoprotein potentially interacts with the human CD26, a key immunoregulatory factor for hijacking and virulence.					
32178593	2	42	theme	modelled	300:307	arg1	structure					321:329	our modelled homo-trimer structure	296:329	our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion	296:467	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	3	43	gly	glycosylation	513:525	arg1	glycoprotein					542:553	spike glycoprotein	536:553	spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system	536:672	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	1	44	theme	urgent	202:207	arg1	outbreak					148:155	The recent outbreak	137:155	The recent outbreak of pneumonia-causing COVID-19 in China	137:194	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	1	44	theme	urgent	202:207	arg1	issue					230:234	an urgent global public health issue	199:234	an urgent global public health issue with an increase in mortality and morbidity	199:278	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	0	45	with	interaction	108:118	arg1	CD26					131:134	human CD26	125:134	human CD26	125:134	Emerging WuHan (COVID-19) coronavirus: glycan shield and structure prediction of spike glycoprotein and its interaction with human CD26.					
32178593	1	46	theme	global	209:214	arg1	outbreak					148:155	The recent outbreak	137:155	The recent outbreak of pneumonia-causing COVID-19 in China	137:194	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	1	46	theme	global	209:214	arg1	issue					230:234	an urgent global public health issue	199:234	an urgent global public health issue with an increase in mortality and morbidity	199:278	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	3	47	link	O-linked	504:511	arg1	glycosylation					513:525	O-linked glycosylation	504:525	O-linked glycosylation	504:525	We also predict the unique N- and O-linked glycosylation sites of spike glycoprotein that distinguish it from the SARS and underlines shielding and camouflage of COVID-19 from the host the defence system.					
32178593	1	48	from	increase	244:251	arg1	morbidity					270:278	morbidity	270:278	morbidity	270:278	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	1	48	from	increase	244:251	arg1	mortality					256:264	mortality	256:264	mortality	256:264	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	1	49	theme	public	216:221	arg1	outbreak					148:155	The recent outbreak	137:155	The recent outbreak of pneumonia-causing COVID-19 in China	137:194	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	1	49	theme	public	216:221	arg1	issue					230:234	an urgent global public health issue	199:234	an urgent global public health issue with an increase in mortality and morbidity	199:278	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	2	50	theme	cell	455:458	arg1	adhesion					460:467	host cell adhesion	450:467	host cell adhesion	450:467	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	1	51	from	outbreak	148:155	arg1	China					190:194	China	190:194	China	190:194	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	2	52	theme	spike	343:347	arg1	glycoprotein					349:360	COVID-19 spike glycoprotein	334:360	COVID-19 spike glycoprotein	334:360	Here we report our modelled homo-trimer structure of COVID-19 spike glycoprotein in both closed (ligand-free) and open (ligand-bound) conformation, which is involved in host cell adhesion.					
32178593	1	53	theme	health	223:228	arg1	outbreak					148:155	The recent outbreak	137:155	The recent outbreak of pneumonia-causing COVID-19 in China	137:194	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
32178593	1	53	theme	health	223:228	arg1	issue					230:234	an urgent global public health issue	199:234	an urgent global public health issue with an increase in mortality and morbidity	199:278	The recent outbreak of pneumonia-causing COVID-19 in China is an urgent global public health issue with an increase in mortality and morbidity.					
33104960	7	0	theme	Phylogenetic	1039:1050	arg1	comparison					1052:1061	Phylogenetic comparison	1039:1061	Phylogenetic comparison using multiple sequence alignment between MIK1	1039:1108	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	3	1	theme	MIK1	486:489	arg1	purification					431:442	purification	431:442	purification	431:442	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	3	1	theme	MIK1	486:489	arg1	expression					416:425	expression	416:425	expression	416:425	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	1	2	theme	such	225:228	arg1	proteins					230:237	such proteins	225:237	such proteins	225:237	An abundance of protein structures has been solved in the last six decades that are paramount in defining the function of such proteins.					
33104960	0	3	theme	KINASE1	87:93	arg1	purification					12:23	purification	12:23	purification	12:23	Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1).					
33104960	0	3	theme	KINASE1	87:93	arg1	Expression					0:9	Expression	0:9	Expression	0:9	Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1).					
33104960	0	3	theme	KINASE1	87:93	arg1	analysis					43:50	phylogenetic analysis	30:50	phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1)	30:100	Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1).					
33104960	5	4	from	analysis	830:837	arg1	MIK1					855:858	MIK1	855:858	MIK1	855:858	Our experimental analysis of N-glycans on MIK1 identified clusters of glycosylation that may explicate the regions involved in MIK1 ectodomain binding.					
33104960	4	5	theme	glycosylation	728:740	arg1	specificity					698:708	glycosylation site specificity	679:708	glycosylation site specificity	679:708	Though highly ubiquitous, glycosylation site specificity and the degree of glycosylation influenced by genetic and exogenous factors are still largely unknown.					
33104960	4	5	theme	glycosylation	728:740	arg1	degree					718:723	the degree	714:723	the degree of glycosylation influenced by genetic and exogenous factors	714:784	Though highly ubiquitous, glycosylation site specificity and the degree of glycosylation influenced by genetic and exogenous factors are still largely unknown.					
33104960	4	5	theme	glycosylation	728:740	arg1	unknown					804:810	unknown	804:810	unknown	804:810	Though highly ubiquitous, glycosylation site specificity and the degree of glycosylation influenced by genetic and exogenous factors are still largely unknown.					
33104960	1	6	theme	proteins	230:237	arg1	function					213:220	the function	209:220	the function of such proteins	209:237	An abundance of protein structures has been solved in the last six decades that are paramount in defining the function of such proteins.					
33104960	7	7	theme	conserved	1175:1183	arg1	motifs					1196:1201	conserved structural motifs	1175:1201	conserved structural motifs that are known to play functional roles in ligand and receptor binding	1175:1272	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	5	8	theme	ectodomain	945:954	arg1	binding					956:962	MIK1 ectodomain binding	940:962	MIK1 ectodomain binding	940:962	Our experimental analysis of N-glycans on MIK1 identified clusters of glycosylation that may explicate the regions involved in MIK1 ectodomain binding.					
33104960	7	9	from	TDR	1137:1139	arg1	thaliana					1156:1163	Arabidopsis thaliana	1144:1163	Arabidopsis thaliana	1144:1163	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	3	10	theme	glycosylation	572:584	arg1	analysis					586:593	subsequent N-linked glycosylation analysis	552:593	subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses	552:650	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	7	11	theme	structural	1185:1194	arg1	motifs					1196:1201	conserved structural motifs	1175:1201	conserved structural motifs that are known to play functional roles in ligand and receptor binding	1175:1272	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	4	12	theme	exogenous	768:776	arg1	factors					778:784	genetic and exogenous factors	756:784	genetic and exogenous factors	756:784	Though highly ubiquitous, glycosylation site specificity and the degree of glycosylation influenced by genetic and exogenous factors are still largely unknown.					
33104960	3	13	theme	plant	461:465	arg1	MIK1					486:489	a recombinant plant LRR-RLK ectodomain MIK1	447:489	a recombinant plant LRR-RLK ectodomain MIK1	447:489	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	4	14	theme	genetic	756:762	arg1	factors					778:784	genetic and exogenous factors	756:784	genetic and exogenous factors	756:784	Though highly ubiquitous, glycosylation site specificity and the degree of glycosylation influenced by genetic and exogenous factors are still largely unknown.					
33104960	2	15	theme	protein	253:259	arg1	structures					261:270	unsolved protein structures	244:270	unsolved protein structures	244:270	For unsolved protein structures, however, predictions based on sequence and phylogenetic similarity can be useful for identifying key domains of interaction.					
33104960	7	16	theme	receptor	1257:1264	arg1	binding					1266:1272	ligand and receptor binding	1246:1272	binding	1266:1272	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	2	17	theme	unsolved	244:251	arg1	structures					261:270	unsolved protein structures	244:270	unsolved protein structures	244:270	For unsolved protein structures, however, predictions based on sequence and phylogenetic similarity can be useful for identifying key domains of interaction.					
33104960	1	18	theme	last	161:164	arg1	decades					170:176	the last six decades	157:176	the last six decades that are paramount in defining the function of such proteins	157:237	An abundance of protein structures has been solved in the last six decades that are paramount in defining the function of such proteins.					
33104960	1	18	theme	last	161:164	arg1	paramount					187:195	paramount	187:195	paramount	187:195	An abundance of protein structures has been solved in the last six decades that are paramount in defining the function of such proteins.					
33104960	5	19	theme	experimental	817:828	arg1	analysis					830:837	Our experimental analysis	813:837	Our experimental analysis of N-glycans on MIK1	813:858	Our experimental analysis of N-glycans on MIK1 identified clusters of glycosylation that may explicate the regions involved in MIK1 ectodomain binding.					
33104960	7	20	theme	Arabidopsis	1144:1154	arg1	thaliana					1156:1163	Arabidopsis thaliana	1144:1163	Arabidopsis thaliana	1144:1163	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	4	21	theme	site	693:696	arg1	specificity					698:708	glycosylation site specificity	679:708	glycosylation site specificity	679:708	Though highly ubiquitous, glycosylation site specificity and the degree of glycosylation influenced by genetic and exogenous factors are still largely unknown.					
33104960	2	22	dep	sequence	303:310	arg1	similarity					329:338	similarity	329:338	similarity	329:338	For unsolved protein structures, however, predictions based on sequence and phylogenetic similarity can be useful for identifying key domains of interaction.					
33104960	7	23	from	comparison	1052:1061	arg1	thaliana					1156:1163	Arabidopsis thaliana	1144:1163	Arabidopsis thaliana	1144:1163	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	7	24	theme	ligand	1246:1251	arg1	binding					1266:1272	ligand and receptor binding	1246:1272	binding	1266:1272	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	4	25	gly	glycosylation	679:691	arg2	specificity					698:708	glycosylation site specificity	679:708	glycosylation site specificity	679:708	Though highly ubiquitous, glycosylation site specificity and the degree of glycosylation influenced by genetic and exogenous factors are still largely unknown.					
33104960	4	25	gly	glycosylation	679:691	arg2	site					693:696	glycosylation site specificity	679:708	glycosylation site specificity	679:708	Though highly ubiquitous, glycosylation site specificity and the degree of glycosylation influenced by genetic and exogenous factors are still largely unknown.					
33104960	7	26	theme	functional	1226:1235	arg1	roles					1237:1241	functional roles	1226:1241	functional roles	1226:1241	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	3	27	theme	subsequent	552:561	arg1	analysis					586:593	subsequent N-linked glycosylation analysis	552:593	subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses	552:650	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	5	28	theme	glycosylation	883:895	arg1	clusters					871:878	clusters	871:878	clusters of glycosylation that may explicate the regions involved in MIK1 ectodomain binding	871:962	Our experimental analysis of N-glycans on MIK1 identified clusters of glycosylation that may explicate the regions involved in MIK1 ectodomain binding.					
33104960	3	29	theme	baculovirus-mediated	508:527	arg1	system					540:545	a modified baculovirus-mediated expression system	497:545	a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses	497:650	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	3	30	theme	N-linked	563:570	arg1	analysis					586:593	subsequent N-linked glycosylation analysis	552:593	subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses	552:650	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	0	31	theme	phylogenetic	30:41	arg1	analysis					43:50	phylogenetic analysis	30:50	phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1)	30:100	Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1).					
33104960	4	32	theme	glycosylation	679:691	arg1	specificity					698:708	glycosylation site specificity	679:708	glycosylation site specificity	679:708	Though highly ubiquitous, glycosylation site specificity and the degree of glycosylation influenced by genetic and exogenous factors are still largely unknown.					
33104960	2	33	theme	interaction	385:395	arg1	interaction					385:395	interaction	385:395	interaction	385:395	For unsolved protein structures, however, predictions based on sequence and phylogenetic similarity can be useful for identifying key domains of interaction.					
33104960	2	33	theme	interaction	385:395	arg1	domains					374:380	key domains	370:380	key domains of interaction	370:395	For unsolved protein structures, however, predictions based on sequence and phylogenetic similarity can be useful for identifying key domains of interaction.					
33104960	3	34	theme	computational	614:626	arg1	analyses					643:650	computational sequence-based analyses	614:650	computational sequence-based analyses	614:650	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	3	35	with	system	540:545	arg1	analysis					586:593	subsequent N-linked glycosylation analysis	552:593	subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses	552:650	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	7	36	theme	other	1114:1118	arg1	LRR-RLKs					1120:1127	other LRR-RLKs	1114:1127	other LRR-RLKs	1114:1127	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	3	37	link	N-linked	563:570	arg1	analysis					586:593	subsequent N-linked glycosylation analysis	552:593	subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses	552:650	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	4	38	dep	ubiquitous	667:676	arg1	highly					660:665	highly	660:665	highly	660:665	Though highly ubiquitous, glycosylation site specificity and the degree of glycosylation influenced by genetic and exogenous factors are still largely unknown.					
33104960	3	39	theme	LRR-RLK	467:473	arg1	MIK1					486:489	a recombinant plant LRR-RLK ectodomain MIK1	447:489	a recombinant plant LRR-RLK ectodomain MIK1	447:489	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	7	40	theme	multiple	1069:1076	arg1	alignment					1087:1095	multiple sequence alignment	1069:1095	multiple sequence alignment between MIK1	1069:1108	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	3	41	theme	ectodomain	475:484	arg1	MIK1					486:489	a recombinant plant LRR-RLK ectodomain MIK1	447:489	a recombinant plant LRR-RLK ectodomain MIK1	447:489	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	5	42	theme	N-glycans	842:850	arg1	analysis					830:837	Our experimental analysis	813:837	Our experimental analysis of N-glycans on MIK1	813:858	Our experimental analysis of N-glycans on MIK1 identified clusters of glycosylation that may explicate the regions involved in MIK1 ectodomain binding.					
33104960	7	43	theme	sequence	1078:1085	arg1	alignment					1087:1095	multiple sequence alignment	1069:1095	multiple sequence alignment between MIK1	1069:1108	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
33104960	1	44	theme	protein	119:125	arg1	structures					127:136	protein structures	119:136	protein structures	119:136	An abundance of protein structures has been solved in the last six decades that are paramount in defining the function of such proteins.					
33104960	3	45	theme	expression	529:538	arg1	system					540:545	a modified baculovirus-mediated expression system	497:545	a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses	497:650	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	1	46	theme	structures	127:136	arg1	abundance					106:114	An abundance	103:114	An abundance of protein structures	103:136	An abundance of protein structures has been solved in the last six decades that are paramount in defining the function of such proteins.					
33104960	3	47	theme	recombinant	449:459	arg1	MIK1					486:489	a recombinant plant LRR-RLK ectodomain MIK1	447:489	a recombinant plant LRR-RLK ectodomain MIK1	447:489	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	0	48	theme	RECEPTOR-LIKE	73:85	arg1	KINASE1					87:93	MDIS1-INTERACTING RECEPTOR-LIKE KINASE1	55:93	MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1)	55:100	Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1).					
33104960	0	48	theme	RECEPTOR-LIKE	73:85	arg1	MIK1					96:99	MIK1	96:99	MIK1	96:99	Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1).					
33104960	3	49	theme	sequence-based	628:641	arg1	analyses					643:650	computational sequence-based analyses	614:650	computational sequence-based analyses	614:650	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	5	50	theme	MIK1	940:943	arg1	binding					956:962	MIK1 ectodomain binding	940:962	MIK1 ectodomain binding	940:962	Our experimental analysis of N-glycans on MIK1 identified clusters of glycosylation that may explicate the regions involved in MIK1 ectodomain binding.					
33104960	2	51	theme	key	370:372	arg1	interaction					385:395	interaction	385:395	interaction	385:395	For unsolved protein structures, however, predictions based on sequence and phylogenetic similarity can be useful for identifying key domains of interaction.					
33104960	2	51	theme	key	370:372	arg1	domains					374:380	key domains	370:380	key domains of interaction	370:395	For unsolved protein structures, however, predictions based on sequence and phylogenetic similarity can be useful for identifying key domains of interaction.					
33104960	0	52	theme	MDIS1-INTERACTING	55:71	arg1	KINASE1					87:93	MDIS1-INTERACTING RECEPTOR-LIKE KINASE1	55:93	MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1)	55:100	Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1).					
33104960	0	52	theme	MDIS1-INTERACTING	55:71	arg1	MIK1					96:99	MIK1	96:99	MIK1	96:99	Expression, purification, and phylogenetic analysis of MDIS1-INTERACTING RECEPTOR-LIKE KINASE1 (MIK1).					
33104960	3	53	theme	modified	499:506	arg1	system					540:545	a modified baculovirus-mediated expression system	497:545	a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses	497:650	Here, we describe expression and purification of a recombinant plant LRR-RLK ectodomain MIK1 using a modified baculovirus-mediated expression system with subsequent N-linked glycosylation analysis using LC-MS/MS and computational sequence-based analyses.					
33104960	7	54	from	LRR-RLKs	1120:1127	arg1	thaliana					1156:1163	Arabidopsis thaliana	1144:1163	Arabidopsis thaliana	1144:1163	Phylogenetic comparison using multiple sequence alignment between MIK1 and other LRR-RLKs, namely TDR in Arabidopsis thaliana, revealed conserved structural motifs that are known to play functional roles in ligand and receptor binding.					
32109354	0	0	theme	Oligomannose	74:85	arg1	Glycans					87:93	Oligomannose Glycans	74:93	Oligomannose Glycans	74:93	The Impact of Sustained Immunization Regimens on the Antibody Response to Oligomannose Glycans.					
32109354	6	1	theme	antibody	1188:1195	arg1	response					1197:1204	the strongest HIV Env-binding antibody response	1158:1204	the strongest HIV Env-binding antibody response	1158:1204	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	4	2	theme	Man9	810:813	arg1	availability					787:798	the availability	783:798	the availability of intact Man9 to germinal centers	783:833	We sought to test whether more prolonged dosing could increase the antibody response to intact glycans, possibly by increasing the availability of intact Man9 to germinal centers.					
32109354	2	3	theme	previous	340:347	arg1	efforts					349:355	our previous efforts	336:355	our previous efforts	336:355	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	0	4	from	Impact	4:9	arg1	Response					62:69	the Antibody Response	49:69	the Antibody Response to Oligomannose Glycans	49:93	The Impact of Sustained Immunization Regimens on the Antibody Response to Oligomannose Glycans.					
32109354	2	5	dep	glycopeptides	401:413	arg1	mimic					395:399	mimic	395:399	mimic	395:399	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	1	6	theme	HIV	132:134	arg1	Env					154:156	Env	154:156	Env	154:156	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	1	6	theme	HIV	132:134	arg1	protein					145:151	the HIV envelope protein	128:151	the HIV envelope protein (Env)	128:157	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	1	7	theme	neutralizing	212:223	arg1	antibodies					225:234	broadly neutralizing antibodies	204:234	broadly neutralizing antibodies	204:234	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	2	8	theme	glycan	486:491	arg1	core					493:496	the glycan core	482:496	the glycan core	482:496	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	3	9	theme	host	631:634	arg1	mannosidases					642:653	host serum mannosidases	631:653	host serum mannosidases	631:653	A possible reason could be processing of our immunogen by host serum mannosidases.					
32109354	2	10	theme	Manα1→2Man	540:549	arg1	tips					551:554	the full glycan or Manα1→2Man tips	521:554	tips	551:554	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	6	11	theme	strongest	1162:1170	arg1	response					1197:1204	the strongest HIV Env-binding antibody response	1158:1204	the strongest HIV Env-binding antibody response	1158:1204	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	5	12	theme	immunization	890:901	arg1	regimen					903:909	immunization regimen	890:909	immunization regimen	890:909	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	7	13	theme	bolus-immunized	1393:1407	arg1	animals					1409:1415	the bolus-immunized animals	1389:1415	the bolus-immunized animals	1389:1415	Antibody selectivity for intact glycan was, if anything, slightly better in the bolus-immunized animals.					
32109354	5	14	theme	regimen	903:909	arg1	impact					880:885	the impact	876:885	the impact of immunization regimen on antibody response	876:930	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	6	15	theme	pump	1298:1301	arg1	regimens					1303:1310	pump regimens	1298:1310	pump regimens	1298:1310	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	7	16	theme	Antibody	1313:1320	arg1	selectivity					1322:1332	Antibody selectivity	1313:1332	Antibody selectivity for intact glycan	1313:1350	Antibody selectivity for intact glycan was, if anything, slightly better in the bolus-immunized animals.					
32109354	6	17	theme	glycopeptide	1096:1107	arg1	immunogens					1109:1118	our glycopeptide immunogens	1092:1118	our glycopeptide immunogens	1092:1118	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	1	18	theme	envelope	136:143	arg1	Env					154:156	Env	154:156	Env	154:156	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	1	18	theme	envelope	136:143	arg1	protein					145:151	the HIV envelope protein	128:151	the HIV envelope protein (Env)	128:157	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	1	19	theme	vaccine	314:320	arg1	immunogen					322:330	a vaccine immunogen	312:330	a vaccine immunogen	312:330	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	5	20	theme	antibody	914:921	arg1	response					923:930	antibody response	914:930	antibody response	914:930	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	1	21	theme	protein	145:151	arg1	HMP					120:122	HMP	120:122	HMP	120:122	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	1	21	theme	protein	145:151	arg1	patch					113:117	The high mannose patch	96:117	The high mannose patch (HMP) of the HIV envelope protein (Env)	96:157	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	1	21	theme	protein	145:151	arg1	structure					166:174	the structure	162:174	the structure most frequently targeted by broadly neutralizing antibodies	162:234	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	5	22	theme	mini-osmotic	1041:1052	arg1	pump					1054:1057	a continuously infusing mini-osmotic pump	1017:1057	a continuously infusing mini-osmotic pump	1017:1057	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	2	23	theme	glycans	564:570	arg1	tips					551:554	the full glycan or Manα1→2Man tips	521:554	tips	551:554	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	2	23	theme	glycans	564:570	arg1	glycan					530:535	the full glycan or Manα1→2Man tips	521:554	glycan	530:535	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	2	24	contain	containing	415:424	arg2	Man9					426:429	Man9	426:429	Man9	426:429	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	2	24	contain	containing	415:424	arg1	glycopeptides					401:413	evolved HMP mimic glycopeptides	383:413	evolved HMP mimic glycopeptides containing Man9	383:429	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	2	25	theme	HMP	391:393	arg1	glycopeptides					401:413	evolved HMP mimic glycopeptides	383:413	evolved HMP mimic glycopeptides containing Man9	383:429	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	2	26	theme	Man9	559:562	arg1	glycans					564:570	Man9 glycans	559:570	Man9 glycans	559:570	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	4	27	theme	intact	803:808	arg1	Man9					810:813	intact Man9	803:813	intact Man9	803:813	We sought to test whether more prolonged dosing could increase the antibody response to intact glycans, possibly by increasing the availability of intact Man9 to germinal centers.					
32109354	0	28	theme	Sustained	14:22	arg1	Regimens					37:44	Sustained Immunization Regimens	14:44	Sustained Immunization Regimens	14:44	The Impact of Sustained Immunization Regimens on the Antibody Response to Oligomannose Glycans.					
32109354	6	29	theme	bolus	1130:1134	arg1	immunization					1136:1147	standard bolus immunization	1121:1147	standard bolus immunization	1121:1147	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	4	30	theme	prolonged	687:695	arg1	dosing					697:702	more prolonged dosing	682:702	more prolonged dosing	682:702	We sought to test whether more prolonged dosing could increase the antibody response to intact glycans, possibly by increasing the availability of intact Man9 to germinal centers.					
32109354	1	31	theme	many	248:251	arg1	researchers					253:263	many researchers	248:263	many researchers	248:263	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	5	32	theme	mini	1002:1005	arg1	doses					1007:1011	mini doses	1002:1011	mini doses	1002:1011	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	1	33	theme	high	100:103	arg1	HMP					120:122	HMP	120:122	HMP	120:122	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	1	33	theme	high	100:103	arg1	patch					113:117	The high mannose patch	96:117	The high mannose patch (HMP) of the HIV envelope protein (Env)	96:157	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	1	33	theme	high	100:103	arg1	structure					166:174	the structure	162:174	the structure most frequently targeted by broadly neutralizing antibodies	162:234	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	2	34	theme	vaccinating	358:368	arg1	rabbits					370:376	vaccinating rabbits	358:376	vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9	358:429	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	0	35	theme	Regimens	37:44	arg1	Impact					4:9	The Impact	0:9	The Impact of Sustained Immunization Regimens on the Antibody Response to Oligomannose Glycans	0:93	The Impact of Sustained Immunization Regimens on the Antibody Response to Oligomannose Glycans.					
32109354	5	36	theme	doses	1007:1011	arg1	series					992:997	an exponential series	977:997	an exponential series of mini doses	977:1011	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	5	36	theme	doses	1007:1011	arg1	bolus					970:974	bolus	970:974	bolus	970:974	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	5	36	theme	doses	1007:1011	arg1	pump					1054:1057	a continuously infusing mini-osmotic pump	1017:1057	a continuously infusing mini-osmotic pump	1017:1057	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	6	37	theme	Env-binding	1176:1186	arg1	response					1197:1204	the strongest HIV Env-binding antibody response	1158:1204	the strongest HIV Env-binding antibody response	1158:1204	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	6	38	gly	glycopeptide	1248:1259	arg2	glycopeptide					1248:1259	the glycopeptide	1244:1259	the glycopeptide	1244:1259	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	0	39	theme	Immunization	24:35	arg1	Regimens					37:44	Sustained Immunization Regimens	14:44	Sustained Immunization Regimens	14:44	The Impact of Sustained Immunization Regimens on the Antibody Response to Oligomannose Glycans.					
32109354	3	40	theme	possible	575:582	arg1	reason					584:589	A possible reason	573:589	A possible reason	573:589	A possible reason could be processing of our immunogen by host serum mannosidases.					
32109354	3	40	theme	possible	575:582	arg1	processing					600:609	processing	600:609	processing of our immunogen by host serum mannosidases	600:653	A possible reason could be processing of our immunogen by host serum mannosidases.					
32109354	2	41	theme	evolved	383:389	arg1	glycopeptides					401:413	evolved HMP mimic glycopeptides	383:413	evolved HMP mimic glycopeptides containing Man9	383:429	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	6	42	dep	exponential	1282:1292	arg1	the					1278:1280	the	1278:1280	the	1278:1280	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	0	43	theme	Antibody	53:60	arg1	Response					62:69	the Antibody Response	49:69	the Antibody Response to Oligomannose Glycans	49:93	The Impact of Sustained Immunization Regimens on the Antibody Response to Oligomannose Glycans.					
32109354	2	44	gly	glycopeptides	401:413	arg2	glycopeptides					401:413	evolved HMP mimic glycopeptides	383:413	evolved HMP mimic glycopeptides containing Man9	383:429	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	2	45	with	rabbits	370:376	arg1	glycopeptides					401:413	evolved HMP mimic glycopeptides	383:413	evolved HMP mimic glycopeptides containing Man9	383:429	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	1	46	theme	mannose	105:111	arg1	HMP					120:122	HMP	120:122	HMP	120:122	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	1	46	theme	mannose	105:111	arg1	patch					113:117	The high mannose patch	96:117	The high mannose patch (HMP) of the HIV envelope protein (Env)	96:157	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	1	46	theme	mannose	105:111	arg1	structure					166:174	the structure	162:174	the structure most frequently targeted by broadly neutralizing antibodies	162:234	The high mannose patch (HMP) of the HIV envelope protein (Env) is the structure most frequently targeted by broadly neutralizing antibodies; therefore, many researchers have attempted to use mimics of this region as a vaccine immunogen.					
32109354	6	47	gly	glycopeptide	1096:1107	arg2	glycopeptide					1096:1107	glycopeptide	1096:1107	glycopeptide	1096:1107	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	2	48	theme	full	525:528	arg1	glycan					530:535	the full glycan or Manα1→2Man tips	521:554	glycan	530:535	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	5	49	theme	exponential	980:990	arg1	series					992:997	an exponential series	977:997	an exponential series of mini doses	977:1011	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	4	50	theme	germinal	818:825	arg1	centers					827:833	germinal centers	818:833	germinal centers	818:833	We sought to test whether more prolonged dosing could increase the antibody response to intact glycans, possibly by increasing the availability of intact Man9 to germinal centers.					
32109354	5	51	from	impact	880:885	arg1	response					923:930	antibody response	914:930	antibody response	914:930	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	6	52	theme	standard	1121:1128	arg1	immunization					1136:1147	standard bolus immunization	1121:1147	standard bolus immunization	1121:1147	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	6	53	theme	overall	1226:1232	arg1	titers					1234:1239	higher overall titers	1219:1239	higher overall titers to the glycopeptide	1219:1259	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	3	54	theme	immunogen	618:626	arg1	reason					584:589	A possible reason	573:589	A possible reason	573:589	A possible reason could be processing of our immunogen by host serum mannosidases.					
32109354	3	54	theme	immunogen	618:626	arg1	processing					600:609	processing	600:609	processing of our immunogen by host serum mannosidases	600:653	A possible reason could be processing of our immunogen by host serum mannosidases.					
32109354	6	55	theme	HIV	1172:1174	arg1	response					1197:1204	the strongest HIV Env-binding antibody response	1158:1204	the strongest HIV Env-binding antibody response	1158:1204	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	4	56	theme	antibody	723:730	arg1	response					732:739	the antibody response	719:739	the antibody response to intact glycans	719:757	We sought to test whether more prolonged dosing could increase the antibody response to intact glycans, possibly by increasing the availability of intact Man9 to germinal centers.					
32109354	2	57	theme	antibody	454:461	arg1	response					463:470	an overall antibody response	443:470	an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans	443:570	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	4	58	theme	intact	744:749	arg1	glycans					751:757	intact glycans	744:757	intact glycans	744:757	We sought to test whether more prolonged dosing could increase the antibody response to intact glycans, possibly by increasing the availability of intact Man9 to germinal centers.					
32109354	2	59	theme	overall	446:452	arg1	response					463:470	an overall antibody response	443:470	an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans	443:570	In our previous efforts, vaccinating rabbits with evolved HMP mimic glycopeptides containing Man9 resulted in an overall antibody response targeting the glycan core and linker rather than the full glycan or Manα1→2Man tips of Man9 glycans.					
32109354	3	60	theme	serum	636:640	arg1	mannosidases					642:653	host serum mannosidases	631:653	host serum mannosidases	631:653	A possible reason could be processing of our immunogen by host serum mannosidases.					
32109354	5	61	theme	immunogen	943:951	arg1	delivery					953:960	immunogen delivery	943:960	immunogen delivery	943:960	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	5	62	theme	infusing	1032:1039	arg1	pump					1054:1057	a continuously infusing mini-osmotic pump	1017:1057	a continuously infusing mini-osmotic pump	1017:1057	Here, we describe a study investigating the impact of immunization regimen on antibody response by testing immunogen delivery through bolus, an exponential series of mini doses, or a continuously infusing mini-osmotic pump.					
32109354	6	63	theme	higher	1219:1224	arg1	titers					1234:1239	higher overall titers	1219:1239	higher overall titers to the glycopeptide	1219:1259	Our results indicate that, with our glycopeptide immunogens, standard bolus immunization elicited the strongest HIV Env-binding antibody response, even though higher overall titers to the glycopeptide were elicited by the exponential and pump regimens.					
32109354	7	64	theme	intact	1338:1343	arg1	glycan					1345:1350	intact glycan	1338:1350	intact glycan	1338:1350	Antibody selectivity for intact glycan was, if anything, slightly better in the bolus-immunized animals.					
34094206	1	0	theme	particular	184:193	arg1	attention					195:203	particular attention	184:203	particular attention due to its potential to improve the performance of current cancer biomarkers	184:280	Detecting specific protein glycoforms is attracting particular attention due to its potential to improve the performance of current cancer biomarkers.					
34094206	2	1	theme	peptide	511:517	arg1	moieties					519:526	the recognition both the glycan and peptide moieties	475:526	moieties	519:526	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	6	2	theme	diagnostic	1146:1155	arg1	applications					1173:1184	diagnostic and therapeutic applications	1146:1184	diagnostic and therapeutic applications	1146:1184	This study opens a new route for the identification of aptamers for the binary recognition of glycoproteins, useful for diagnostic and therapeutic applications.					
34094206	4	3	theme	prostate-specific	711:727	arg1	antigen					729:735	prostate-specific antigen	711:735	prostate-specific antigen	711:735	It allows identification of an aptamer that binds with nM affinity to prostate-specific antigen, discriminating it from proteins with a similar glycosylation pattern.					
34094206	6	4	theme	new	1045:1047	arg1	route					1049:1053	a new route	1043:1053	a new route for the identification of aptamers for the binary recognition of glycoproteins	1043:1132	This study opens a new route for the identification of aptamers for the binary recognition of glycoproteins, useful for diagnostic and therapeutic applications.					
34094206	0	5	theme	structural	111:120	arg1	modeling					122:129	structural modeling	111:129	structural modeling	111:129	Aptamers targeting protein-specific glycosylation in tumor biomarkers: general selection, characterization and structural modeling.					
34094206	5	6	gly	glycoprotein	916:927	arg1	glycoprotein					916:927	the glycoprotein	912:927	the glycoprotein	912:927	We also computationally predict the structure of the selected aptamer and characterize its complex with the glycoprotein by docking and molecular dynamics calculations, further supporting the binary recognition event.					
34094206	4	7	theme	glycosylation	785:797	arg1	pattern					799:805	a similar glycosylation pattern	775:805	a similar glycosylation pattern	775:805	It allows identification of an aptamer that binds with nM affinity to prostate-specific antigen, discriminating it from proteins with a similar glycosylation pattern.					
34094206	6	8	theme	therapeutic	1161:1171	arg1	applications					1173:1184	diagnostic and therapeutic applications	1146:1184	diagnostic and therapeutic applications	1146:1184	This study opens a new route for the identification of aptamers for the binary recognition of glycoproteins, useful for diagnostic and therapeutic applications.					
34094206	1	9	theme	due	205:207	arg1	attention					195:203	particular attention	184:203	particular attention due to its potential to improve the performance of current cancer biomarkers	184:280	Detecting specific protein glycoforms is attracting particular attention due to its potential to improve the performance of current cancer biomarkers.					
34094206	6	10	theme	aptamers	1081:1088	arg1	identification					1063:1076	the identification	1059:1076	the identification of aptamers	1059:1088	This study opens a new route for the identification of aptamers for the binary recognition of glycoproteins, useful for diagnostic and therapeutic applications.					
34094206	5	11	theme	molecular	944:952	arg1	calculations					963:974	molecular dynamics calculations	944:974	molecular dynamics calculations	944:974	We also computationally predict the structure of the selected aptamer and characterize its complex with the glycoprotein by docking and molecular dynamics calculations, further supporting the binary recognition event.					
34094206	6	12	theme	glycoproteins	1120:1132	arg1	recognition					1105:1115	the binary recognition	1094:1115	the binary recognition of glycoproteins	1094:1132	This study opens a new route for the identification of aptamers for the binary recognition of glycoproteins, useful for diagnostic and therapeutic applications.					
34094206	2	13	theme	recognition	479:489	arg1	glycan					500:505	the recognition both the glycan and peptide moieties	475:526	glycan	500:505	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	2	14	dep	recognition	479:489	arg1	both					491:494	both	491:494	both	491:494	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	4	15	theme	nM	696:697	arg1	affinity					699:706	nM affinity	696:706	nM affinity	696:706	It allows identification of an aptamer that binds with nM affinity to prostate-specific antigen, discriminating it from proteins with a similar glycosylation pattern.					
34094206	0	16	dep	selection	79:87	arg1	Aptamers					0:7	Aptamers	0:7	Aptamers targeting protein-specific glycosylation in tumor biomarkers	0:68	Aptamers targeting protein-specific glycosylation in tumor biomarkers: general selection, characterization and structural modeling.					
34094206	5	17	theme	aptamer	870:876	arg1	structure					844:852	the structure	840:852	the structure of the selected aptamer	840:876	We also computationally predict the structure of the selected aptamer and characterize its complex with the glycoprotein by docking and molecular dynamics calculations, further supporting the binary recognition event.					
34094206	0	18	theme	protein-specific	19:34	arg1	glycosylation					36:48	protein-specific glycosylation	19:48	protein-specific glycosylation in tumor biomarkers	19:68	Aptamers targeting protein-specific glycosylation in tumor biomarkers: general selection, characterization and structural modeling.					
34094206	5	19	theme	recognition	1007:1017	arg1	event					1019:1023	the binary recognition event	996:1023	the binary recognition event	996:1023	We also computationally predict the structure of the selected aptamer and characterize its complex with the glycoprotein by docking and molecular dynamics calculations, further supporting the binary recognition event.					
34094206	2	20	theme	able	454:457	arg1	receptors					444:452	effective receptors	434:452	effective receptors able to integrate in the recognition both the glycan and peptide moieties	434:526	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	0	21	theme	tumor	53:57	arg1	biomarkers					59:68	tumor biomarkers	53:68	tumor biomarkers	53:68	Aptamers targeting protein-specific glycosylation in tumor biomarkers: general selection, characterization and structural modeling.					
34094206	1	22	gly	glycoforms	159:168	arg1	protein					151:157	specific protein glycoforms	142:168	specific protein glycoforms	142:168	Detecting specific protein glycoforms is attracting particular attention due to its potential to improve the performance of current cancer biomarkers.					
34094206	4	23	theme	aptamer	672:678	arg1	identification					651:664	identification	651:664	identification of an aptamer that binds with nM affinity to prostate-specific antigen, discriminating it from proteins with a similar glycosylation pattern	651:805	It allows identification of an aptamer that binds with nM affinity to prostate-specific antigen, discriminating it from proteins with a similar glycosylation pattern.					
34094206	2	24	theme	receptors	444:452	arg1	development					419:429	the development	415:429	the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties	415:526	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	2	25	theme	effective	434:442	arg1	receptors					444:452	effective receptors	434:452	effective receptors able to integrate in the recognition both the glycan and peptide moieties	434:526	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	6	26	gly	glycoproteins	1120:1132	arg1	glycoproteins					1120:1132	glycoproteins	1120:1132	glycoproteins	1120:1132	This study opens a new route for the identification of aptamers for the binary recognition of glycoproteins, useful for diagnostic and therapeutic applications.					
34094206	3	27	theme	glycosylation	608:620	arg1	site					622:625	the glycosylation site	604:625	the glycosylation site of a protein	604:638	Here we report a method for selecting aptamers toward the glycosylation site of a protein.					
34094206	3	27	theme	glycosylation	608:620	arg1	protein					632:638	a protein	630:638	a protein	630:638	Here we report a method for selecting aptamers toward the glycosylation site of a protein.					
34094206	5	28	theme	dynamics	954:961	arg1	calculations					963:974	molecular dynamics calculations	944:974	molecular dynamics calculations	944:974	We also computationally predict the structure of the selected aptamer and characterize its complex with the glycoprotein by docking and molecular dynamics calculations, further supporting the binary recognition event.					
34094206	5	29	theme	binary	1000:1005	arg1	event					1019:1023	the binary recognition event	996:1023	the binary recognition event	996:1023	We also computationally predict the structure of the selected aptamer and characterize its complex with the glycoprotein by docking and molecular dynamics calculations, further supporting the binary recognition event.					
34094206	0	30	gly	glycosylation	36:48	arg1	biomarkers					59:68	tumor biomarkers	53:68	tumor biomarkers	53:68	Aptamers targeting protein-specific glycosylation in tumor biomarkers: general selection, characterization and structural modeling.					
34094206	1	31	theme	specific	142:149	arg1	glycoforms					159:168	specific protein glycoforms	142:168	specific protein glycoforms	142:168	Detecting specific protein glycoforms is attracting particular attention due to its potential to improve the performance of current cancer biomarkers.					
34094206	4	32	with	proteins	761:768	arg1	pattern					799:805	a similar glycosylation pattern	775:805	a similar glycosylation pattern	775:805	It allows identification of an aptamer that binds with nM affinity to prostate-specific antigen, discriminating it from proteins with a similar glycosylation pattern.					
34094206	5	33	with	complex	899:905	arg1	glycoprotein					916:927	the glycoprotein	912:927	the glycoprotein	912:927	We also computationally predict the structure of the selected aptamer and characterize its complex with the glycoprotein by docking and molecular dynamics calculations, further supporting the binary recognition event.					
34094206	1	34	theme	protein	151:157	arg1	glycoforms					159:168	specific protein glycoforms	142:168	specific protein glycoforms	142:168	Detecting specific protein glycoforms is attracting particular attention due to its potential to improve the performance of current cancer biomarkers.					
34094206	4	35	theme	similar	777:783	arg1	pattern					799:805	a similar glycosylation pattern	775:805	a similar glycosylation pattern	775:805	It allows identification of an aptamer that binds with nM affinity to prostate-specific antigen, discriminating it from proteins with a similar glycosylation pattern.					
34094206	2	36	theme	natural	292:298	arg1	lectins					318:324	lectins	318:324	lectins	318:324	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	2	36	theme	natural	292:298	arg1	antibodies					330:339	antibodies	330:339	antibodies	330:339	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	2	36	theme	natural	292:298	arg1	receptors					300:308	natural receptors	292:308	natural receptors such as lectins and antibodies	292:339	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	2	37	theme	powerful	356:363	arg1	tools					365:369	powerful tools	356:369	powerful tools for the detection of protein-bound glycans	356:412	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	1	38	theme	current	256:262	arg1	biomarkers					271:280	current cancer biomarkers	256:280	current cancer biomarkers	256:280	Detecting specific protein glycoforms is attracting particular attention due to its potential to improve the performance of current cancer biomarkers.					
34094206	3	39	gly	glycosylation	608:620	arg1	protein					632:638	a protein	630:638	a protein	630:638	Here we report a method for selecting aptamers toward the glycosylation site of a protein.					
34094206	3	39	gly	glycosylation	608:620	arg2	site					622:625	the glycosylation site	604:625	the glycosylation site of a protein	604:638	Here we report a method for selecting aptamers toward the glycosylation site of a protein.					
34094206	3	39	gly	glycosylation	608:620	arg2	protein					632:638	a protein	630:638	a protein	630:638	Here we report a method for selecting aptamers toward the glycosylation site of a protein.					
34094206	3	40	theme	protein	632:638	arg1	site					622:625	the glycosylation site	604:625	the glycosylation site of a protein	604:638	Here we report a method for selecting aptamers toward the glycosylation site of a protein.					
34094206	3	40	theme	protein	632:638	arg1	protein					632:638	a protein	630:638	a protein	630:638	Here we report a method for selecting aptamers toward the glycosylation site of a protein.					
34094206	6	41	theme	binary	1098:1103	arg1	recognition					1105:1115	the binary recognition	1094:1115	the binary recognition of glycoproteins	1094:1132	This study opens a new route for the identification of aptamers for the binary recognition of glycoproteins, useful for diagnostic and therapeutic applications.					
34094206	2	42	theme	glycans	406:412	arg1	detection					379:387	the detection	375:387	the detection of protein-bound glycans	375:412	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	1	43	theme	cancer	264:269	arg1	biomarkers					271:280	current cancer biomarkers	256:280	current cancer biomarkers	256:280	Detecting specific protein glycoforms is attracting particular attention due to its potential to improve the performance of current cancer biomarkers.					
34094206	0	44	theme	general	71:77	arg1	selection					79:87	general selection	71:87	general selection	71:87	Aptamers targeting protein-specific glycosylation in tumor biomarkers: general selection, characterization and structural modeling.					
34094206	0	45	from	glycosylation	36:48	arg1	biomarkers					59:68	tumor biomarkers	53:68	tumor biomarkers	53:68	Aptamers targeting protein-specific glycosylation in tumor biomarkers: general selection, characterization and structural modeling.					
34094206	5	46	theme	selected	861:868	arg1	aptamer					870:876	the selected aptamer	857:876	the selected aptamer	857:876	We also computationally predict the structure of the selected aptamer and characterize its complex with the glycoprotein by docking and molecular dynamics calculations, further supporting the binary recognition event.					
34094206	2	47	theme	protein-bound	392:404	arg1	glycans					406:412	protein-bound glycans	392:412	protein-bound glycans	392:412	Although natural receptors such as lectins and antibodies have served as powerful tools for the detection of protein-bound glycans, the development of effective receptors able to integrate in the recognition both the glycan and peptide moieties is still challenging.					
34094206	1	48	theme	biomarkers	271:280	arg1	performance					241:251	the performance	237:251	the performance of current cancer biomarkers	237:280	Detecting specific protein glycoforms is attracting particular attention due to its potential to improve the performance of current cancer biomarkers.					
33351642	2	0	theme	stereodirecting	317:331	arg1	participation					333:345	stereodirecting participation	317:345	stereodirecting participation	317:345	This finding indicated that stereodirecting participation is not limited to groups within the glycan ring being activated, thus broadening the scope of remote group participation in glycosylation.					
33351642	1	1	theme	macrocyclic	143:153	arg1	ketal					155:159	an unprecedented 12-membered macrocyclic ketal	114:159	an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage	114:246	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	1	2	theme	unprecedented	117:129	arg1	ketal					155:159	an unprecedented 12-membered macrocyclic ketal	114:159	an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage	114:246	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	1	3	theme	glycosidic	229:238	arg1	linkage					240:246	a glycosidic linkage	227:246	a glycosidic linkage	227:246	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	0	4	theme	Glycosidic	50:59	arg1	Linkage					61:67	a Glycosidic Linkage	48:67	a Glycosidic Linkage in Glycosylation Reactions	48:94	Long-Range Stereodirecting Participation across a Glycosidic Linkage in Glycosylation Reactions.					
33351642	2	5	theme	glycan	383:388	arg1	ring					390:393	the glycan ring	379:393	the glycan ring	379:393	This finding indicated that stereodirecting participation is not limited to groups within the glycan ring being activated, thus broadening the scope of remote group participation in glycosylation.					
33351642	1	6	theme	ketal	155:159	arg1	formation					101:109	The formation	97:109	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage	97:246	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	1	7	theme	levulinoyl	203:212	arg1	group					214:218	a levulinoyl group	201:218	a levulinoyl group across a glycosidic linkage	201:246	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	2	8	from	scope	432:436	arg1	glycosylation					471:483	glycosylation	471:483	glycosylation	471:483	This finding indicated that stereodirecting participation is not limited to groups within the glycan ring being activated, thus broadening the scope of remote group participation in glycosylation.					
33351642	2	9	theme	group	448:452	arg1	participation					454:466	remote group participation	441:466	remote group participation in glycosylation	441:483	This finding indicated that stereodirecting participation is not limited to groups within the glycan ring being activated, thus broadening the scope of remote group participation in glycosylation.					
33351642	1	10	theme	long-range	173:182	arg1	participation					184:196	the long-range participation	169:196	the long-range participation of a levulinoyl group across a glycosidic linkage	169:246	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	1	11	theme	group	214:218	arg1	participation					184:196	the long-range participation	169:196	the long-range participation of a levulinoyl group across a glycosidic linkage	169:246	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	1	12	located	observed	252:259	arg1	reactions					278:286	glycosylation reactions	264:286	glycosylation reactions	264:286	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	1	12	located	observed	252:259	arg2	formation					101:109	The formation	97:109	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage	97:246	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	2	13	from	glycosylation	471:483	arg1	scope					432:436	the scope	428:436	the scope of remote group participation in glycosylation	428:483	This finding indicated that stereodirecting participation is not limited to groups within the glycan ring being activated, thus broadening the scope of remote group participation in glycosylation.					
33351642	2	14	theme	remote	441:446	arg1	participation					454:466	remote group participation	441:466	remote group participation in glycosylation	441:483	This finding indicated that stereodirecting participation is not limited to groups within the glycan ring being activated, thus broadening the scope of remote group participation in glycosylation.					
33351642	2	15	from	participation	454:466	arg1	glycosylation					471:483	glycosylation	471:483	glycosylation	471:483	This finding indicated that stereodirecting participation is not limited to groups within the glycan ring being activated, thus broadening the scope of remote group participation in glycosylation.					
33351642	1	16	theme	glycosylation	264:276	arg1	reactions					278:286	glycosylation reactions	264:286	glycosylation reactions	264:286	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	0	17	theme	Glycosylation	72:84	arg1	Reactions					86:94	Glycosylation Reactions	72:94	Glycosylation Reactions	72:94	Long-Range Stereodirecting Participation across a Glycosidic Linkage in Glycosylation Reactions.					
33351642	1	18	theme	12-membered	131:141	arg1	ketal					155:159	an unprecedented 12-membered macrocyclic ketal	114:159	an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage	114:246	The formation of an unprecedented 12-membered macrocyclic ketal through the long-range participation of a levulinoyl group across a glycosidic linkage was observed in glycosylation reactions.					
33351642	0	19	from	Linkage	61:67	arg1	Reactions					86:94	Glycosylation Reactions	72:94	Glycosylation Reactions	72:94	Long-Range Stereodirecting Participation across a Glycosidic Linkage in Glycosylation Reactions.					
33351642	2	20	theme	participation	454:466	arg1	scope					432:436	the scope	428:436	the scope of remote group participation in glycosylation	428:483	This finding indicated that stereodirecting participation is not limited to groups within the glycan ring being activated, thus broadening the scope of remote group participation in glycosylation.					
33758080	4	0	theme	tyrosine	670:677	arg1	sulfation					679:687	tyrosine sulfation	670:687	tyrosine sulfation	670:687	Our findings reveal that the GPR15 receptor is sulfated on the N-terminal tyrosine residue(s) and disruption of tyrosine sulfation inhibits binding of GPR15L.					
33758080	6	1	theme	N-terminal	976:985	arg1	PTMs					987:990	different N-terminal PTMs	966:990	different N-terminal PTMs	966:990	Thus, GPR15 represents a unique chemoattractant receptor in which different N-terminal PTMs regulate its ligand binding in a contrasting manner.					
33758080	5	2	theme	GPR15L	884:889	arg1	binding					891:897	the GPR15L binding	880:897	the GPR15L binding	880:897	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	7	3	theme	GPR15L	1103:1108	arg1	activity					1110:1117	GPR15L activity	1103:1117	GPR15L activity	1103:1117	We further demonstrate that, unlike canonical chemokines, GPR15L activity critically requires its extreme C-terminal residue and that its hydrophobicity may be a key attribute that facilitates an optimal interaction with the receptor.					
33758080	3	4	theme	receptor	501:508	arg1	N-terminus					510:519	receptor N-terminus	501:519	receptor N-terminus	501:519	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	6	5	theme	different	966:974	arg1	PTMs					987:990	different N-terminal PTMs	966:990	different N-terminal PTMs	966:990	Thus, GPR15 represents a unique chemoattractant receptor in which different N-terminal PTMs regulate its ligand binding in a contrasting manner.					
33758080	8	6	theme	GPR15-GPR15L	1432:1443	arg1	axis					1445:1448	the GPR15-GPR15L axis	1428:1448	the GPR15-GPR15L axis	1428:1448	Our results reveal novel insights into chemoattractant receptor-ligand interaction and provide a valid footing for potential intervention targeting the GPR15-GPR15L axis.					
33758080	7	7	theme	canonical	1081:1089	arg1	chemokines					1091:1100	canonical chemokines	1081:1100	canonical chemokines	1081:1100	We further demonstrate that, unlike canonical chemokines, GPR15L activity critically requires its extreme C-terminal residue and that its hydrophobicity may be a key attribute that facilitates an optimal interaction with the receptor.					
33758080	0	8	with	interaction	97:107	arg1	GPR15L					114:119	GPR15L	114:119	GPR15L	114:119	Tyrosine sulfation and O-glycosylation of chemoattractant receptor GPR15 differentially regulate interaction with GPR15L.					
33758080	3	9	theme	ligand	550:555	arg1	C-terminus					532:541	the C-terminus	528:541	the C-terminus of the ligand	528:555	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	5	10	from	removal	819:825	arg1	threonine					782:790	the N-terminal threonine or serine residues	767:809	threonine	782:790	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	10	from	removal	819:825	arg1	residues					802:809	the N-terminal threonine or serine residues	767:809	residues	802:809	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	8	11	theme	chemoattractant	1319:1333	arg1	interaction					1351:1361	chemoattractant receptor-ligand interaction	1319:1361	chemoattractant receptor-ligand interaction	1319:1361	Our results reveal novel insights into chemoattractant receptor-ligand interaction and provide a valid footing for potential intervention targeting the GPR15-GPR15L axis.					
33758080	7	12	with	interaction	1249:1259	arg1	receptor					1270:1277	the receptor	1266:1277	the receptor	1266:1277	We further demonstrate that, unlike canonical chemokines, GPR15L activity critically requires its extreme C-terminal residue and that its hydrophobicity may be a key attribute that facilitates an optimal interaction with the receptor.					
33758080	5	13	from	O-glycans	860:868	arg1	removal					819:825	the removal	815:825	the removal of α2,3-linked sialic acids from O-glycans	815:868	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	13	from	O-glycans	860:868	arg1	disruption					734:743	the disruption	730:743	the disruption of O-glycosylation on the N-terminal threonine or serine residues	730:809	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	14	theme	sialic	842:847	arg1	acids					849:853	α2,3-linked sialic acids	830:853	α2,3-linked sialic acids	830:853	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	1	15	theme	lymphocyte	180:189	arg1	homing					191:196	lymphocyte homing	180:196	lymphocyte homing	180:196	GPR15 is a G-protein-coupled receptor (GPCR) that directs lymphocyte homing to the colon and skin.					
33758080	5	16	theme	N-terminal	771:780	arg1	threonine					782:790	the N-terminal threonine or serine residues	767:809	threonine	782:790	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	17	theme	O-glycosylation	748:762	arg1	removal					819:825	the removal	815:825	the removal of α2,3-linked sialic acids from O-glycans	815:868	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	17	theme	O-glycosylation	748:762	arg1	disruption					734:743	the disruption	730:743	the disruption of O-glycosylation on the N-terminal threonine or serine residues	730:809	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	18	theme	α2,3-linked	830:840	arg1	acids					849:853	α2,3-linked sialic acids	830:853	α2,3-linked sialic acids	830:853	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	8	19	theme	valid	1377:1381	arg1	footing					1383:1389	a valid footing	1375:1389	a valid footing for potential intervention targeting the GPR15-GPR15L axis	1375:1448	Our results reveal novel insights into chemoattractant receptor-ligand interaction and provide a valid footing for potential intervention targeting the GPR15-GPR15L axis.					
33758080	2	20	dep	GPR15L	277:282	arg1	known					290:294	known	290:294	known as C10orf99	290:306	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	0	21	theme	Tyrosine	0:7	arg1	sulfation					9:17	Tyrosine sulfation	0:17	Tyrosine sulfation	0:17	Tyrosine sulfation and O-glycosylation of chemoattractant receptor GPR15 differentially regulate interaction with GPR15L.					
33758080	5	22	link	α2,3-linked	830:840	arg1	acids					849:853	α2,3-linked sialic acids	830:853	α2,3-linked sialic acids	830:853	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	4	23	theme	GPR15L	709:714	arg1	binding					698:704	binding	698:704	binding of GPR15L	698:714	Our findings reveal that the GPR15 receptor is sulfated on the N-terminal tyrosine residue(s) and disruption of tyrosine sulfation inhibits binding of GPR15L.					
33758080	3	24	theme	GPR15-GPR15L	411:422	arg1	interaction					424:434	the GPR15-GPR15L interaction	407:434	the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand	407:555	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	5	25	theme	serine	795:800	arg1	residues					802:809	the N-terminal threonine or serine residues	767:809	residues	802:809	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	6	26	theme	chemoattractant	932:946	arg1	receptor					948:955	a unique chemoattractant receptor	923:955	a unique chemoattractant receptor in which different N-terminal PTMs regulate its ligand binding in a contrasting manner	923:1042	Thus, GPR15 represents a unique chemoattractant receptor in which different N-terminal PTMs regulate its ligand binding in a contrasting manner.					
33758080	0	27	gly	O-glycosylation	23:37	arg1	GPR15					67:71	chemoattractant receptor GPR15	42:71	chemoattractant receptor GPR15	42:71	Tyrosine sulfation and O-glycosylation of chemoattractant receptor GPR15 differentially regulate interaction with GPR15L.					
33758080	6	28	theme	contrasting	1025:1035	arg1	manner					1037:1042	a contrasting manner	1023:1042	a contrasting manner	1023:1042	Thus, GPR15 represents a unique chemoattractant receptor in which different N-terminal PTMs regulate its ligand binding in a contrasting manner.					
33758080	7	29	theme	C-terminal	1151:1160	arg1	residue					1162:1168	its extreme C-terminal residue	1139:1168	its extreme C-terminal residue	1139:1168	We further demonstrate that, unlike canonical chemokines, GPR15L activity critically requires its extreme C-terminal residue and that its hydrophobicity may be a key attribute that facilitates an optimal interaction with the receptor.					
33758080	6	30	theme	unique	925:930	arg1	receptor					948:955	a unique chemoattractant receptor	923:955	a unique chemoattractant receptor in which different N-terminal PTMs regulate its ligand binding in a contrasting manner	923:1042	Thus, GPR15 represents a unique chemoattractant receptor in which different N-terminal PTMs regulate its ligand binding in a contrasting manner.					
33758080	2	31	dep	known	290:294	arg1	also					285:288	also	285:288	also	285:288	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	3	32	theme	post-translational	458:475	arg1	PTMs					492:495	PTMs	492:495	PTMs	492:495	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	3	32	theme	post-translational	458:475	arg1	modifications					477:489	post-translational modifications	458:489	post-translational modifications (PTMs) of receptor N-terminus	458:519	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	0	33	theme	chemoattractant	42:56	arg1	GPR15					67:71	chemoattractant receptor GPR15	42:71	chemoattractant receptor GPR15	42:71	Tyrosine sulfation and O-glycosylation of chemoattractant receptor GPR15 differentially regulate interaction with GPR15L.					
33758080	4	34	theme	sulfation	679:687	arg1	disruption					656:665	disruption	656:665	disruption of tyrosine sulfation	656:687	Our findings reveal that the GPR15 receptor is sulfated on the N-terminal tyrosine residue(s) and disruption of tyrosine sulfation inhibits binding of GPR15L.					
33758080	4	35	theme	tyrosine	632:639	arg1	s					649:649	s	649:649	s	649:649	Our findings reveal that the GPR15 receptor is sulfated on the N-terminal tyrosine residue(s) and disruption of tyrosine sulfation inhibits binding of GPR15L.					
33758080	4	35	theme	tyrosine	632:639	arg1	residue					641:647	the N-terminal tyrosine residue	617:647	the N-terminal tyrosine residue(s)	617:650	Our findings reveal that the GPR15 receptor is sulfated on the N-terminal tyrosine residue(s) and disruption of tyrosine sulfation inhibits binding of GPR15L.					
33758080	5	36	theme	acids	849:853	arg1	removal					819:825	the removal	815:825	the removal of α2,3-linked sialic acids from O-glycans	815:868	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	36	theme	acids	849:853	arg1	disruption					734:743	the disruption	730:743	the disruption of O-glycosylation on the N-terminal threonine or serine residues	730:809	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	3	37	theme	primary	441:447	arg1	focus					449:453	primary focus	441:453	primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand	441:555	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	8	38	theme	novel	1299:1303	arg1	insights					1305:1312	novel insights	1299:1312	novel insights into chemoattractant receptor-ligand interaction	1299:1361	Our results reveal novel insights into chemoattractant receptor-ligand interaction and provide a valid footing for potential intervention targeting the GPR15-GPR15L axis.					
33758080	7	39	theme	extreme	1143:1149	arg1	residue					1162:1168	its extreme C-terminal residue	1139:1168	its extreme C-terminal residue	1139:1168	We further demonstrate that, unlike canonical chemokines, GPR15L activity critically requires its extreme C-terminal residue and that its hydrophobicity may be a key attribute that facilitates an optimal interaction with the receptor.					
33758080	4	40	theme	N-terminal	621:630	arg1	s					649:649	s	649:649	s	649:649	Our findings reveal that the GPR15 receptor is sulfated on the N-terminal tyrosine residue(s) and disruption of tyrosine sulfation inhibits binding of GPR15L.					
33758080	4	40	theme	N-terminal	621:630	arg1	residue					641:647	the N-terminal tyrosine residue	617:647	the N-terminal tyrosine residue(s)	617:650	Our findings reveal that the GPR15 receptor is sulfated on the N-terminal tyrosine residue(s) and disruption of tyrosine sulfation inhibits binding of GPR15L.					
33758080	3	41	theme	structural	373:382	arg1	elements					384:391	the structural elements	369:391	the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand	369:555	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	3	42	from	focus	449:453	arg1	C-terminus					532:541	the C-terminus	528:541	the C-terminus of the ligand	528:555	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	3	42	from	focus	449:453	arg1	PTMs					492:495	PTMs	492:495	PTMs	492:495	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	3	42	from	focus	449:453	arg1	modifications					477:489	post-translational modifications	458:489	post-translational modifications (PTMs) of receptor N-terminus	458:519	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	2	43	theme	GPR15	335:339	arg1	ligand					325:330	a functional ligand	312:330	a functional ligand of GPR15	312:339	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	2	43	theme	GPR15	335:339	arg1	GPR15L					277:282	a chemokine-like protein GPR15L	252:282	a chemokine-like protein GPR15L (also known as C10orf99)	252:307	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	0	44	theme	receptor	58:65	arg1	GPR15					67:71	chemoattractant receptor GPR15	42:71	chemoattractant receptor GPR15	42:71	Tyrosine sulfation and O-glycosylation of chemoattractant receptor GPR15 differentially regulate interaction with GPR15L.					
33758080	5	45	from	disruption	734:743	arg1	threonine					782:790	the N-terminal threonine or serine residues	767:809	threonine	782:790	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	45	from	disruption	734:743	arg1	residues					802:809	the N-terminal threonine or serine residues	767:809	residues	802:809	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	2	46	theme	Recent	221:226	arg1	studies					228:234	Recent studies	221:234	Recent studies	221:234	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	1	47	theme	G-protein-coupled	133:149	arg1	receptor					151:158	a G-protein-coupled receptor	131:158	a G-protein-coupled receptor (GPCR) that directs lymphocyte homing to the colon and skin	131:218	GPR15 is a G-protein-coupled receptor (GPCR) that directs lymphocyte homing to the colon and skin.					
33758080	1	47	theme	G-protein-coupled	133:149	arg1	GPR15					122:126	GPR15	122:126	GPR15	122:126	GPR15 is a G-protein-coupled receptor (GPCR) that directs lymphocyte homing to the colon and skin.					
33758080	1	47	theme	G-protein-coupled	133:149	arg1	GPCR					161:164	GPCR	161:164	GPCR	161:164	GPR15 is a G-protein-coupled receptor (GPCR) that directs lymphocyte homing to the colon and skin.					
33758080	3	48	with	interaction	424:434	arg1	focus					449:453	primary focus	441:453	primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand	441:555	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	8	49	theme	potential	1395:1403	arg1	intervention					1405:1416	potential intervention	1395:1416	potential intervention targeting the GPR15-GPR15L axis	1395:1448	Our results reveal novel insights into chemoattractant receptor-ligand interaction and provide a valid footing for potential intervention targeting the GPR15-GPR15L axis.					
33758080	6	50	theme	ligand	1005:1010	arg1	binding					1012:1018	its ligand binding	1001:1018	its ligand binding	1001:1018	Thus, GPR15 represents a unique chemoattractant receptor in which different N-terminal PTMs regulate its ligand binding in a contrasting manner.					
33758080	5	51	attach	removal	819:825	arg2	acids					849:853	α2,3-linked sialic acids	830:853	α2,3-linked sialic acids	830:853	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	51	attach	removal	819:825	arg3	residues					802:809	the N-terminal threonine or serine residues	767:809	residues	802:809	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	51	attach	removal	819:825	arg3	threonine					782:790	the N-terminal threonine or serine residues	767:809	threonine	782:790	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	5	51	attach	removal	819:825	arg2	O-glycosylation					748:762	O-glycosylation	748:762	O-glycosylation	748:762	In contrast, the disruption of O-glycosylation on the N-terminal threonine or serine residues, or the removal of α2,3-linked sialic acids from O-glycans, enhances the GPR15L binding.					
33758080	2	52	theme	protein	269:275	arg1	ligand					325:330	a functional ligand	312:330	a functional ligand of GPR15	312:339	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	2	52	theme	protein	269:275	arg1	GPR15L					277:282	a chemokine-like protein GPR15L	252:282	a chemokine-like protein GPR15L (also known as C10orf99)	252:307	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	2	53	theme	functional	314:323	arg1	ligand					325:330	a functional ligand	312:330	a functional ligand of GPR15	312:339	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	2	53	theme	functional	314:323	arg1	GPR15L					277:282	a chemokine-like protein GPR15L	252:282	a chemokine-like protein GPR15L (also known as C10orf99)	252:307	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	8	54	theme	receptor-ligand	1335:1349	arg1	interaction					1351:1361	chemoattractant receptor-ligand interaction	1319:1361	chemoattractant receptor-ligand interaction	1319:1361	Our results reveal novel insights into chemoattractant receptor-ligand interaction and provide a valid footing for potential intervention targeting the GPR15-GPR15L axis.					
33758080	4	55	theme	GPR15	587:591	arg1	receptor					593:600	the GPR15 receptor	583:600	the GPR15 receptor	583:600	Our findings reveal that the GPR15 receptor is sulfated on the N-terminal tyrosine residue(s) and disruption of tyrosine sulfation inhibits binding of GPR15L.					
33758080	7	56	theme	key	1207:1209	arg1	hydrophobicity					1183:1196	its hydrophobicity	1179:1196	its hydrophobicity	1179:1196	We further demonstrate that, unlike canonical chemokines, GPR15L activity critically requires its extreme C-terminal residue and that its hydrophobicity may be a key attribute that facilitates an optimal interaction with the receptor.					
33758080	7	56	theme	key	1207:1209	arg1	attribute					1211:1219	a key attribute	1205:1219	a key attribute that facilitates an optimal interaction with the receptor	1205:1277	We further demonstrate that, unlike canonical chemokines, GPR15L activity critically requires its extreme C-terminal residue and that its hydrophobicity may be a key attribute that facilitates an optimal interaction with the receptor.					
33758080	2	57	theme	chemokine-like	254:267	arg1	ligand					325:330	a functional ligand	312:330	a functional ligand of GPR15	312:339	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	2	57	theme	chemokine-like	254:267	arg1	GPR15L					277:282	a chemokine-like protein GPR15L	252:282	a chemokine-like protein GPR15L (also known as C10orf99)	252:307	Recent studies have identified a chemokine-like protein GPR15L (also known as C10orf99) as a functional ligand of GPR15.					
33758080	3	58	theme	N-terminus	510:519	arg1	PTMs					492:495	PTMs	492:495	PTMs	492:495	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	3	58	theme	N-terminus	510:519	arg1	modifications					477:489	post-translational modifications	458:489	post-translational modifications (PTMs) of receptor N-terminus	458:519	In this study, we examined the structural elements that regulate the GPR15-GPR15L interaction with primary focus on post-translational modifications (PTMs) of receptor N-terminus and on the C-terminus of the ligand.					
33758080	0	59	theme	GPR15	67:71	arg1	O-glycosylation					23:37	O-glycosylation	23:37	O-glycosylation of chemoattractant receptor GPR15	23:71	Tyrosine sulfation and O-glycosylation of chemoattractant receptor GPR15 differentially regulate interaction with GPR15L.					
33758080	0	59	theme	GPR15	67:71	arg1	sulfation					9:17	Tyrosine sulfation	0:17	Tyrosine sulfation	0:17	Tyrosine sulfation and O-glycosylation of chemoattractant receptor GPR15 differentially regulate interaction with GPR15L.					
33758080	7	60	theme	optimal	1241:1247	arg1	interaction					1249:1259	an optimal interaction	1238:1259	an optimal interaction with the receptor	1238:1277	We further demonstrate that, unlike canonical chemokines, GPR15L activity critically requires its extreme C-terminal residue and that its hydrophobicity may be a key attribute that facilitates an optimal interaction with the receptor.					
32204644	10	0	theme	glycosyl	1600:1607	arg1	residues					1609:1616	glycosyl residues	1600:1616	glycosyl residues	1600:1616	Our approach facilitates the characterization of enzymes that modify glycosyl residues during cell wall synthesis and the structures that they generate.					
32204644	6	1	theme	Function	955:962	arg1	Domain					937:942	the Domain	933:942	the Domain of Unknown Function (DUF) 579 family	933:979	Members of the Domain of Unknown Function (DUF) 579 family have been demonstrated to have a role in the biosynthesis of methyl-etherified glycans.					
32204644	6	1	theme	Function	955:962	arg1	Function					955:962	Unknown Function	947:962	Unknown Function (DUF) 579 family	947:979	Members of the Domain of Unknown Function (DUF) 579 family have been demonstrated to have a role in the biosynthesis of methyl-etherified glycans.					
32204644	7	2	theme	moieties	1145:1152	arg1	analysis					1102:1109	the analysis	1098:1109	the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins	1098:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	2	3	theme	arabinogalactan	350:364	arg1	Glucuronoxylan					331:344	Glucuronoxylan	331:344	Glucuronoxylan	331:344	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	2	3	theme	arabinogalactan	350:364	arg1	protein					366:372	arabinogalactan protein	350:372	arabinogalactan protein	350:372	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	2	3	theme	arabinogalactan	350:364	arg1	glycans					385:391	matrix glycans	378:391	matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues	378:460	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	0	4	theme	Function	68:75	arg1	Proteins					81:88	Unknown Function 579 Proteins	60:88	Unknown Function 579 Proteins	60:88	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides.					
32204644	6	5	theme	Unknown	947:953	arg1	DUF					965:967	DUF	965:967	DUF	965:967	Members of the Domain of Unknown Function (DUF) 579 family have been demonstrated to have a role in the biosynthesis of methyl-etherified glycans.					
32204644	6	5	theme	Unknown	947:953	arg1	Function					955:962	Unknown Function	947:962	Unknown Function (DUF) 579 family	947:979	Members of the Domain of Unknown Function (DUF) 579 family have been demonstrated to have a role in the biosynthesis of methyl-etherified glycans.					
32204644	8	6	theme	DUF579	1300:1305	arg1	members					1314:1320	two DUF579 family members	1296:1320	two DUF579 family members that lack this modification in muro	1296:1356	These methods are then applied toward the analysis of loss-of-function mutants of two DUF579 family members that lack this modification in muro.					
32204644	4	7	from	distribution	713:724	arg1	species					796:802	different plant species	780:802	different plant species	780:802	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	4	7	from	distribution	713:724	arg1	stages					832:837	developmental stages	818:837	developmental stages	818:837	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	4	7	from	distribution	713:724	arg1	tissues					805:811	tissues	805:811	tissues	805:811	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	7	8	from	sidechains	1174:1183	arg1	moieties					1145:1152	the 4-O-methyl glucuronic acid moieties	1114:1152	the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins	1114:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	8	from	sidechains	1174:1183	arg1	present					1163:1169	present	1163:1169	present	1163:1169	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	9	theme	glucuronic	1129:1138	arg1	moieties					1145:1152	the 4-O-methyl glucuronic acid moieties	1114:1152	the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins	1114:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	9	theme	glucuronic	1129:1138	arg1	present					1163:1169	present	1163:1169	present	1163:1169	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	10	located	present	1163:1169	arg2	moieties					1145:1152	the 4-O-methyl glucuronic acid moieties	1114:1152	the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins	1114:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	10	located	present	1163:1169	arg1	sidechains					1174:1183	sidechains	1174:1183	sidechains of arabinogalactan proteins	1174:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	10	located	present	1163:1169	arg2	present					1163:1169	present	1163:1169	present	1163:1169	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	0	11	theme	Proteins	81:88	arg1	Proteins					81:88	Unknown Function 579 Proteins	60:88	Unknown Function 579 Proteins	60:88	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides.					
32204644	0	11	theme	Proteins	81:88	arg1	Domain					50:55	Domain	50:55	Domain of Unknown Function 579 Proteins	50:88	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides.					
32204644	6	12	theme	Domain	937:942	arg1	Members					922:928	Members	922:928	Members of the Domain of Unknown Function (DUF) 579 family	922:979	Members of the Domain of Unknown Function (DUF) 579 family have been demonstrated to have a role in the biosynthesis of methyl-etherified glycans.					
32204644	7	13	theme	acid	1140:1143	arg1	moieties					1145:1152	the 4-O-methyl glucuronic acid moieties	1114:1152	the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins	1114:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	13	theme	acid	1140:1143	arg1	present					1163:1169	present	1163:1169	present	1163:1169	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	14	attach	present	1163:1169	arg1	sidechains					1174:1183	sidechains	1174:1183	sidechains of arabinogalactan proteins	1174:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	14	attach	present	1163:1169	arg2	moieties					1145:1152	the 4-O-methyl glucuronic acid moieties	1114:1152	the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins	1114:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	14	attach	present	1163:1169	arg2	present					1163:1169	present	1163:1169	present	1163:1169	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	1	15	theme	biomass	266:272	arg1	portion					251:257	a large portion	243:257	a large portion of the biomass consumed as food or used to produce fuels and materials	243:328	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	10	16	theme	wall	1630:1633	arg1	synthesis					1635:1643	cell wall synthesis	1625:1643	cell wall synthesis	1625:1643	Our approach facilitates the characterization of enzymes that modify glycosyl residues during cell wall synthesis and the structures that they generate.					
32204644	4	17	theme	methyl-etherified	743:759	arg1	polysaccharides					761:775	methyl-etherified polysaccharides	743:775	methyl-etherified polysaccharides	743:775	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	7	18	theme	arabinogalactan	1188:1202	arg1	proteins					1204:1211	arabinogalactan proteins	1188:1211	arabinogalactan proteins	1188:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	9	19	theme	loss-of-function	1505:1520	arg1	mutants					1522:1528	loss-of-function mutants	1505:1528	loss-of-function mutants	1505:1528	We also present a procedure to assay DUF579 family members for enzymatic activity in vitro using acceptor oligosaccharides prepared from xylan of loss-of-function mutants.					
32204644	4	20	theme	developmental	818:830	arg1	stages					832:837	developmental stages	818:837	developmental stages	818:837	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	8	21	theme	loss-of-function	1268:1283	arg1	mutants					1285:1291	loss-of-function mutants	1268:1291	loss-of-function mutants of two DUF579 family members that lack this modification in muro	1268:1356	These methods are then applied toward the analysis of loss-of-function mutants of two DUF579 family members that lack this modification in muro.					
32204644	6	22	dep	Function	955:962	arg1	family					974:979	579 family	970:979	Unknown Function (DUF) 579 family	947:979	Members of the Domain of Unknown Function (DUF) 579 family have been demonstrated to have a role in the biosynthesis of methyl-etherified glycans.					
32204644	0	23	from	Role	42:45	arg1	Synthesis					97:105	the Synthesis	93:105	the Synthesis of O-Methylated Plant Polysaccharides	93:143	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides.					
32204644	7	24	theme	4-O-methyl	1118:1127	arg1	moieties					1145:1152	the 4-O-methyl glucuronic acid moieties	1114:1152	the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins	1114:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	7	24	theme	4-O-methyl	1118:1127	arg1	present					1163:1169	present	1163:1169	present	1163:1169	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	1	25	theme	diverse	175:181	arg1	group					183:187	a diverse group	173:187	a diverse group of structurally complex carbohydrates	173:225	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	1	25	theme	diverse	175:181	arg1	polysaccharides					153:167	Matrix polysaccharides	146:167	Matrix polysaccharides	146:167	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	1	25	theme	diverse	175:181	arg1	carbohydrates					213:225	structurally complex carbohydrates	192:225	structurally complex carbohydrates	192:225	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	7	26	theme	proteins	1204:1211	arg1	sidechains					1174:1183	sidechains	1174:1183	sidechains of arabinogalactan proteins	1174:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	4	27	theme	plant	790:794	arg1	species					796:802	different plant species	780:802	different plant species	780:802	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	0	28	theme	Analytical	0:9	arg1	Techniques					11:20	Analytical Techniques	0:20	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides	0:143	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides.					
32204644	4	29	theme	polysaccharides	761:775	arg1	abundance					730:738	abundance	730:738	abundance	730:738	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	4	29	theme	polysaccharides	761:775	arg1	distribution					713:724	distribution	713:724	distribution	713:724	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	4	30	theme	different	780:788	arg1	species					796:802	different plant species	780:802	different plant species	780:802	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	2	31	theme	matrix	378:383	arg1	Glucuronoxylan					331:344	Glucuronoxylan	331:344	Glucuronoxylan	331:344	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	2	31	theme	matrix	378:383	arg1	protein					366:372	arabinogalactan protein	350:372	arabinogalactan protein	350:372	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	2	31	theme	matrix	378:383	arg1	glycans					385:391	matrix glycans	378:391	matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues	378:460	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	0	32	theme	O-Methylated	110:121	arg1	Polysaccharides					129:143	O-Methylated Plant Polysaccharides	110:143	O-Methylated Plant Polysaccharides	110:143	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides.					
32204644	2	33	theme	4-O-methyl	429:438	arg1	residues					453:460	4-O-methyl glucuronosyl residues	429:460	4-O-methyl glucuronosyl residues	429:460	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	2	34	theme	glucuronosyl	440:451	arg1	residues					453:460	4-O-methyl glucuronosyl residues	429:460	4-O-methyl glucuronosyl residues	429:460	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	4	35	dep	distribution	713:724	arg1	the					709:711	the	709:711	the	709:711	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	3	36	theme	biological	587:596	arg1	function					598:605	their biological function	581:605	their biological function	581:605	Methylation is a key determinant of the physical properties of these wall glycopolymers and consequently affects both their biological function and ability to interact with other wall polymers.					
32204644	9	37	theme	acceptor	1456:1463	arg1	oligosaccharides					1465:1480	acceptor oligosaccharides	1456:1480	acceptor oligosaccharides prepared from xylan of loss-of-function mutants	1456:1528	We also present a procedure to assay DUF579 family members for enzymatic activity in vitro using acceptor oligosaccharides prepared from xylan of loss-of-function mutants.					
32204644	1	38	theme	complex	205:211	arg1	carbohydrates					213:225	structurally complex carbohydrates	192:225	structurally complex carbohydrates	192:225	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	10	39	theme	cell	1625:1628	arg1	synthesis					1635:1643	cell wall synthesis	1625:1643	cell wall synthesis	1625:1643	Our approach facilitates the characterization of enzymes that modify glycosyl residues during cell wall synthesis and the structures that they generate.					
32204644	0	40	theme	Polysaccharides	129:143	arg1	Synthesis					97:105	the Synthesis	93:105	the Synthesis of O-Methylated Plant Polysaccharides	93:143	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides.					
32204644	8	41	theme	members	1314:1320	arg1	mutants					1285:1291	loss-of-function mutants	1268:1291	loss-of-function mutants of two DUF579 family members that lack this modification in muro	1268:1356	These methods are then applied toward the analysis of loss-of-function mutants of two DUF579 family members that lack this modification in muro.					
32204644	8	42	theme	mutants	1285:1291	arg1	analysis					1256:1263	the analysis	1252:1263	the analysis of loss-of-function mutants of two DUF579 family members that lack this modification in muro	1252:1356	These methods are then applied toward the analysis of loss-of-function mutants of two DUF579 family members that lack this modification in muro.					
32204644	1	43	theme	carbohydrates	213:225	arg1	group					183:187	a diverse group	173:187	a diverse group of structurally complex carbohydrates	173:225	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	1	43	theme	carbohydrates	213:225	arg1	polysaccharides					153:167	Matrix polysaccharides	146:167	Matrix polysaccharides	146:167	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	1	43	theme	carbohydrates	213:225	arg1	carbohydrates					213:225	structurally complex carbohydrates	192:225	structurally complex carbohydrates	192:225	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	0	44	theme	Plant	123:127	arg1	Polysaccharides					129:143	O-Methylated Plant Polysaccharides	110:143	O-Methylated Plant Polysaccharides	110:143	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides.					
32204644	6	45	theme	glycans	1060:1066	arg1	biosynthesis					1026:1037	the biosynthesis	1022:1037	the biosynthesis of methyl-etherified glycans	1022:1066	Members of the Domain of Unknown Function (DUF) 579 family have been demonstrated to have a role in the biosynthesis of methyl-etherified glycans.					
32204644	0	46	theme	Domain	50:55	arg1	Role					42:45	the Role	38:45	the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides	38:143	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides.					
32204644	6	47	contain	have	1007:1010	arg1	Members					922:928	Members	922:928	Members of the Domain of Unknown Function (DUF) 579 family	922:979	Members of the Domain of Unknown Function (DUF) 579 family have been demonstrated to have a role in the biosynthesis of methyl-etherified glycans.					
32204644	6	47	contain	have	1007:1010	arg2	role					1014:1017	a role	1012:1017	a role	1012:1017	Members of the Domain of Unknown Function (DUF) 579 family have been demonstrated to have a role in the biosynthesis of methyl-etherified glycans.					
32204644	2	48	contain	have	398:401	arg1	Glucuronoxylan					331:344	Glucuronoxylan	331:344	Glucuronoxylan	331:344	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	2	48	contain	have	398:401	arg1	protein					366:372	arabinogalactan protein	350:372	arabinogalactan protein	350:372	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	2	48	contain	have	398:401	arg1	glycans					385:391	matrix glycans	378:391	matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues	378:460	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	2	48	contain	have	398:401	arg2	sidechains					403:412	sidechains	403:412	sidechains decorated with 4-O-methyl glucuronosyl residues	403:460	Glucuronoxylan and arabinogalactan protein are matrix glycans that have sidechains decorated with 4-O-methyl glucuronosyl residues.					
32204644	9	49	theme	mutants	1522:1528	arg1	xylan					1496:1500	xylan	1496:1500	xylan of loss-of-function mutants	1496:1528	We also present a procedure to assay DUF579 family members for enzymatic activity in vitro using acceptor oligosaccharides prepared from xylan of loss-of-function mutants.					
32204644	3	50	theme	physical	503:510	arg1	properties					512:521	the physical properties	499:521	the physical properties of these wall glycopolymers	499:549	Methylation is a key determinant of the physical properties of these wall glycopolymers and consequently affects both their biological function and ability to interact with other wall polymers.					
32204644	10	51	theme	enzymes	1580:1586	arg1	characterization					1560:1575	the characterization	1556:1575	the characterization of enzymes that modify glycosyl residues during cell wall synthesis and the structures that they generate	1556:1681	Our approach facilitates the characterization of enzymes that modify glycosyl residues during cell wall synthesis and the structures that they generate.					
32204644	3	52	theme	properties	512:521	arg1	Methylation					463:473	Methylation	463:473	Methylation	463:473	Methylation is a key determinant of the physical properties of these wall glycopolymers and consequently affects both their biological function and ability to interact with other wall polymers.					
32204644	3	52	theme	properties	512:521	arg1	determinant					484:494	a key determinant	478:494	a key determinant of the physical properties of these wall glycopolymers	478:549	Methylation is a key determinant of the physical properties of these wall glycopolymers and consequently affects both their biological function and ability to interact with other wall polymers.					
32204644	9	53	theme	family	1403:1408	arg1	members					1410:1416	DUF579 family members	1396:1416	DUF579 family members for enzymatic activity	1396:1439	We also present a procedure to assay DUF579 family members for enzymatic activity in vitro using acceptor oligosaccharides prepared from xylan of loss-of-function mutants.					
32204644	1	54	dep	biomass	266:272	arg1	consumed					274:281	consumed	274:281	consumed as food	274:289	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	1	54	dep	biomass	266:272	arg1	used					294:297	used	294:297	used to produce fuels and materials	294:328	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	7	55	from	present	1163:1169	arg1	sidechains					1174:1183	sidechains	1174:1183	sidechains of arabinogalactan proteins	1174:1211	Here we describe methods for the analysis of the 4-O-methyl glucuronic acid moieties that are present in sidechains of arabinogalactan proteins.					
32204644	6	56	theme	methyl-etherified	1042:1058	arg1	glycans					1060:1066	methyl-etherified glycans	1042:1066	methyl-etherified glycans	1042:1066	Members of the Domain of Unknown Function (DUF) 579 family have been demonstrated to have a role in the biosynthesis of methyl-etherified glycans.					
32204644	3	57	theme	glycopolymers	537:549	arg1	properties					512:521	the physical properties	499:521	the physical properties of these wall glycopolymers	499:549	Methylation is a key determinant of the physical properties of these wall glycopolymers and consequently affects both their biological function and ability to interact with other wall polymers.					
32204644	0	58	theme	Unknown	60:66	arg1	Proteins					81:88	Unknown Function 579 Proteins	60:88	Unknown Function 579 Proteins	60:88	Analytical Techniques for Determining the Role of Domain of Unknown Function 579 Proteins in the Synthesis of O-Methylated Plant Polysaccharides.					
32204644	8	59	theme	family	1307:1312	arg1	members					1314:1320	two DUF579 family members	1296:1320	two DUF579 family members that lack this modification in muro	1296:1356	These methods are then applied toward the analysis of loss-of-function mutants of two DUF579 family members that lack this modification in muro.					
32204644	3	60	theme	other	636:640	arg1	polymers					647:654	other wall polymers	636:654	other wall polymers	636:654	Methylation is a key determinant of the physical properties of these wall glycopolymers and consequently affects both their biological function and ability to interact with other wall polymers.					
32204644	3	61	theme	key	480:482	arg1	Methylation					463:473	Methylation	463:473	Methylation	463:473	Methylation is a key determinant of the physical properties of these wall glycopolymers and consequently affects both their biological function and ability to interact with other wall polymers.					
32204644	3	61	theme	key	480:482	arg1	determinant					484:494	a key determinant	478:494	a key determinant of the physical properties of these wall glycopolymers	478:549	Methylation is a key determinant of the physical properties of these wall glycopolymers and consequently affects both their biological function and ability to interact with other wall polymers.					
32204644	3	62	theme	wall	532:535	arg1	glycopolymers					537:549	these wall glycopolymers	526:549	these wall glycopolymers	526:549	Methylation is a key determinant of the physical properties of these wall glycopolymers and consequently affects both their biological function and ability to interact with other wall polymers.					
32204644	4	63	theme	increasing	674:683	arg1	interest					685:692	increasing interest	674:692	increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages	674:837	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	9	64	theme	enzymatic	1422:1430	arg1	activity					1432:1439	enzymatic activity	1422:1439	enzymatic activity	1422:1439	We also present a procedure to assay DUF579 family members for enzymatic activity in vitro using acceptor oligosaccharides prepared from xylan of loss-of-function mutants.					
32204644	3	65	theme	wall	642:645	arg1	polymers					647:654	other wall polymers	636:654	other wall polymers	636:654	Methylation is a key determinant of the physical properties of these wall glycopolymers and consequently affects both their biological function and ability to interact with other wall polymers.					
32204644	1	66	theme	Matrix	146:151	arg1	group					183:187	a diverse group	173:187	a diverse group of structurally complex carbohydrates	173:225	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	1	66	theme	Matrix	146:151	arg1	carbohydrates					213:225	structurally complex carbohydrates	192:225	structurally complex carbohydrates	192:225	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	1	66	theme	Matrix	146:151	arg1	polysaccharides					153:167	Matrix polysaccharides	146:167	Matrix polysaccharides	146:167	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
32204644	4	67	from	abundance	730:738	arg1	species					796:802	different plant species	780:802	different plant species	780:802	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	4	67	from	abundance	730:738	arg1	stages					832:837	developmental stages	818:837	developmental stages	818:837	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	4	67	from	abundance	730:738	arg1	tissues					805:811	tissues	805:811	tissues	805:811	Indeed, there is increasing interest in determining the distribution and abundance of methyl-etherified polysaccharides in different plant species, tissues, and developmental stages.					
32204644	1	68	theme	large	245:249	arg1	portion					251:257	a large portion	243:257	a large portion of the biomass consumed as food or used to produce fuels and materials	243:328	Matrix polysaccharides are a diverse group of structurally complex carbohydrates and account for a large portion of the biomass consumed as food or used to produce fuels and materials.					
34189908	0	0	theme	Thioacid-Mediated	93:109	arg1	Strategy					111:118	a Bifunctional Thioacid-Mediated Strategy	78:118	a Bifunctional Thioacid-Mediated Strategy	78:118	Glycoprotein Semisynthesis by Chemical Insertion of Glycosyl Asparagine Using a Bifunctional Thioacid-Mediated Strategy.					
34189908	6	1	theme	glycoprotein	975:986	arg1	methods					998:1004	Previous glycoprotein synthesis methods	966:1004	Previous glycoprotein synthesis methods	966:1004	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	7	2	gly	glycoprotein	1390:1401	arg1	glycoprotein					1390:1401	robust glycoprotein semisynthesis	1383:1415	robust glycoprotein semisynthesis	1383:1415	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	6	3	theme	developed	1130:1138	arg1	concept					1140:1146	the developed concept	1126:1146	the developed concept	1126:1146	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	1	4	theme	cell	175:178	arg1	proteins					188:195	cell surface proteins	175:195	cell surface proteins	175:195	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	6	5	gly	glycoproteins	1161:1173	arg1	glycoproteins					1161:1173	glycoproteins	1161:1173	glycoproteins	1161:1173	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	4	6	theme	disulfide	622:630	arg1	coupling					632:639	diacyl disulfide coupling	615:639	diacyl disulfide coupling (DDC)	615:645	We found that diacyl disulfide coupling (DDC) formed with glycosyl asparagine thioacid and peptide thioacid yielded glycopeptides.					
34189908	4	6	theme	disulfide	622:630	arg1	DDC					642:644	DDC	642:644	DDC	642:644	We found that diacyl disulfide coupling (DDC) formed with glycosyl asparagine thioacid and peptide thioacid yielded glycopeptides.					
34189908	0	7	theme	Bifunctional	80:91	arg1	Strategy					111:118	a Bifunctional Thioacid-Mediated Strategy	78:118	a Bifunctional Thioacid-Mediated Strategy	78:118	Glycoprotein Semisynthesis by Chemical Insertion of Glycosyl Asparagine Using a Bifunctional Thioacid-Mediated Strategy.					
34189908	6	8	theme	Previous	966:973	arg1	methods					998:1004	Previous glycoprotein synthesis methods	966:1004	Previous glycoprotein synthesis methods	966:1004	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	1	9	theme	surface	180:186	arg1	proteins					188:195	cell surface proteins	175:195	cell surface proteins	175:195	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	4	10	theme	diacyl	615:620	arg1	coupling					632:639	diacyl disulfide coupling	615:639	diacyl disulfide coupling (DDC)	615:645	We found that diacyl disulfide coupling (DDC) formed with glycosyl asparagine thioacid and peptide thioacid yielded glycopeptides.					
34189908	4	10	theme	diacyl	615:620	arg1	DDC					642:644	DDC	642:644	DDC	642:644	We found that diacyl disulfide coupling (DDC) formed with glycosyl asparagine thioacid and peptide thioacid yielded glycopeptides.					
34189908	6	11	theme	few	1184:1186	arg1	steps					1188:1192	a few steps	1182:1192	a few steps from peptide and glycosyl asparagine thioacid	1182:1238	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	3	12	theme	key	561:563	arg1	reactions					565:573	several key reactions	553:573	several key reactions based on amino thioacids	553:598	To establish a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins, we studied several key reactions based on amino thioacids.					
34189908	3	13	theme	amino	584:588	arg1	thioacids					590:598	amino thioacids	584:598	amino thioacids	584:598	To establish a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins, we studied several key reactions based on amino thioacids.					
34189908	4	14	gly	glycopeptides	717:729	arg2	glycopeptides					717:729	yielded glycopeptides	709:729	yielded glycopeptides	709:729	We found that diacyl disulfide coupling (DDC) formed with glycosyl asparagine thioacid and peptide thioacid yielded glycopeptides.					
34189908	5	15	theme	asparagine	945:954	arg1	thioacid					956:963	glycosyl asparagine thioacid	936:963	glycosyl asparagine thioacid	936:963	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	7	16	theme	thioacid	1359:1366	arg1	ability					1328:1334	the efficient ability	1314:1334	the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis	1314:1415	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	7	16	theme	thioacid	1359:1366	arg1	characterization					1263:1278	the characterization	1259:1278	the characterization of the DDC of amino thioacids	1259:1308	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	5	17	theme	new	789:791	arg1	method					816:821	a new glycoprotein synthesis method	787:821	a new glycoprotein synthesis method	787:821	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	6	18	theme	asparagine	1220:1229	arg1	thioacid					1231:1238	glycosyl asparagine thioacid	1211:1238	glycosyl asparagine thioacid	1211:1238	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	4	19	theme	glycosyl	659:666	arg1	thioacid					679:686	glycosyl asparagine thioacid	659:686	glycosyl asparagine thioacid	659:686	We found that diacyl disulfide coupling (DDC) formed with glycosyl asparagine thioacid and peptide thioacid yielded glycopeptides.					
34189908	7	20	theme	efficient	1318:1326	arg1	ability					1328:1334	the efficient ability	1314:1334	the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis	1314:1415	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	1	21	theme	proteins	188:195	arg1	modification					146:157	a major modification	138:157	a major modification of secreted and cell surface proteins	138:195	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	1	21	theme	proteins	188:195	arg1	Glycosylation					121:133	Glycosylation	121:133	Glycosylation	121:133	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	5	22	theme	glycoprotein	793:804	arg1	method					816:821	a new glycoprotein synthesis method	787:821	a new glycoprotein synthesis method	787:821	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	5	23	theme	thioacid	956:963	arg1	N-					916:917	N-	916:917	N-	916:917	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	6	24	theme	multiple	1078:1085	arg1	synthesis					1100:1108	subsequent multiple glycoprotein synthesis	1067:1108	subsequent multiple glycoprotein synthesis	1067:1108	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	7	25	theme	glycoprotein	1390:1401	arg1	semisynthesis					1403:1415	robust glycoprotein semisynthesis	1383:1415	robust glycoprotein semisynthesis	1383:1415	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	2	26	theme	biological	295:304	arg1	processes					306:314	the biological processes	291:314	the biological processes arising from each glycoform	291:342	To understand the biological processes arising from each glycoform, the preparation of homogeneous glycoproteins is essential for extensive biological experiments.					
34189908	6	27	dep	stage	1057:1061	arg1	routes					1110:1115	routes	1110:1115	routes	1110:1115	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	4	28	theme	peptide	692:698	arg1	thioacid					700:707	peptide thioacid	692:707	peptide thioacid	692:707	We found that diacyl disulfide coupling (DDC) formed with glycosyl asparagine thioacid and peptide thioacid yielded glycopeptides.					
34189908	6	29	from	peptide	1199:1205	arg1	steps					1188:1192	a few steps	1182:1192	a few steps from peptide and glycosyl asparagine thioacid	1182:1238	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	7	30	theme	thioacids	1300:1308	arg1	DDC					1287:1289	the DDC	1283:1289	the DDC of amino thioacids	1283:1308	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	0	31	theme	Glycoprotein	0:11	arg1	Semisynthesis					13:25	Glycoprotein Semisynthesis	0:25	Glycoprotein Semisynthesis by Chemical Insertion of Glycosyl Asparagine	0:70	Glycoprotein Semisynthesis by Chemical Insertion of Glycosyl Asparagine Using a Bifunctional Thioacid-Mediated Strategy.					
34189908	6	32	theme	early	1051:1055	arg1	stage					1057:1061	the early stage	1047:1061	the early stage	1047:1061	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	2	33	theme	biological	417:426	arg1	experiments					428:438	extensive biological experiments	407:438	extensive biological experiments	407:438	To understand the biological processes arising from each glycoform, the preparation of homogeneous glycoproteins is essential for extensive biological experiments.					
34189908	0	34	theme	Chemical	30:37	arg1	Insertion					39:47	Chemical Insertion	30:47	Chemical Insertion of Glycosyl Asparagine	30:70	Glycoprotein Semisynthesis by Chemical Insertion of Glycosyl Asparagine Using a Bifunctional Thioacid-Mediated Strategy.					
34189908	3	35	gly	glycoproteins	527:539	arg1	glycoproteins					527:539	homogeneous glycoproteins	515:539	homogeneous glycoproteins	515:539	To establish a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins, we studied several key reactions based on amino thioacids.					
34189908	6	36	from	thioacid	1231:1238	arg1	steps					1188:1192	a few steps	1182:1192	a few steps from peptide and glycosyl asparagine thioacid	1182:1238	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	1	37	theme	resultant	206:214	arg1	glycans					216:222	the resultant glycans	202:222	the resultant glycans	202:222	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	6	38	from	asparagine	1033:1042	arg1	synthesis					1100:1108	subsequent multiple glycoprotein synthesis	1067:1108	subsequent multiple glycoprotein synthesis	1067:1108	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	6	38	from	asparagine	1033:1042	arg1	stage					1057:1061	the early stage	1047:1061	the early stage	1047:1061	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	3	39	theme	several	553:559	arg1	reactions					565:573	several key reactions	553:573	several key reactions based on amino thioacids	553:598	To establish a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins, we studied several key reactions based on amino thioacids.					
34189908	1	40	theme	secreted	162:169	arg1	modification					146:157	a major modification	138:157	a major modification of secreted and cell surface proteins	138:195	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	1	40	theme	secreted	162:169	arg1	Glycosylation					121:133	Glycosylation	121:133	Glycosylation	121:133	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	7	41	theme	DDC	1287:1289	arg1	ability					1328:1334	the efficient ability	1314:1334	the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis	1314:1415	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	7	41	theme	DDC	1287:1289	arg1	characterization					1263:1278	the characterization	1259:1278	the characterization of the DDC of amino thioacids	1259:1308	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	2	42	theme	homogeneous	364:374	arg1	glycoproteins					376:388	homogeneous glycoproteins	364:388	homogeneous glycoproteins	364:388	To understand the biological processes arising from each glycoform, the preparation of homogeneous glycoproteins is essential for extensive biological experiments.					
34189908	6	43	theme	glycosyl	1211:1218	arg1	thioacid					1231:1238	glycosyl asparagine thioacid	1211:1238	glycosyl asparagine thioacid	1211:1238	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	3	44	theme	rapid	472:476	arg1	route					488:492	a more robust and rapid synthetic route	454:492	a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins	454:539	To establish a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins, we studied several key reactions based on amino thioacids.					
34189908	3	45	theme	robust	461:466	arg1	route					488:492	a more robust and rapid synthetic route	454:492	a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins	454:539	To establish a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins, we studied several key reactions based on amino thioacids.					
34189908	6	46	theme	glycosyl	1024:1031	arg1	asparagine					1033:1042	valuable glycosyl asparagine	1015:1042	valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes	1015:1115	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	6	47	theme	subsequent	1067:1076	arg1	synthesis					1100:1108	subsequent multiple glycoprotein synthesis	1067:1108	subsequent multiple glycoprotein synthesis	1067:1108	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	4	48	dep	thioacid	679:686	arg1	glycopeptides					717:729	yielded glycopeptides	709:729	yielded glycopeptides	709:729	We found that diacyl disulfide coupling (DDC) formed with glycosyl asparagine thioacid and peptide thioacid yielded glycopeptides.					
34189908	1	49	theme	considerable	229:240	arg1	heterogeneity					242:254	considerable heterogeneity	229:254	considerable heterogeneity in their structures	229:274	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	0	50	theme	Asparagine	61:70	arg1	Insertion					39:47	Chemical Insertion	30:47	Chemical Insertion of Glycosyl Asparagine	30:70	Glycoprotein Semisynthesis by Chemical Insertion of Glycosyl Asparagine Using a Bifunctional Thioacid-Mediated Strategy.					
34189908	6	51	theme	valuable	1015:1022	arg1	asparagine					1033:1042	valuable glycosyl asparagine	1015:1042	valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes	1015:1115	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	5	52	theme	thioacid-mediated	849:865	arg1	strategy					867:874	the bifunctional thioacid-mediated strategy	832:874	the bifunctional thioacid-mediated strategy	832:874	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	3	53	theme	synthetic	478:486	arg1	route					488:492	a more robust and rapid synthetic route	454:492	a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins	454:539	To establish a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins, we studied several key reactions based on amino thioacids.					
34189908	5	54	theme	efficient	737:745	arg1	reaction					756:763	This efficient coupling reaction	732:763	This efficient coupling reaction	732:763	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	2	55	theme	glycoproteins	376:388	arg1	essential					393:401	essential	393:401	essential	393:401	To understand the biological processes arising from each glycoform, the preparation of homogeneous glycoproteins is essential for extensive biological experiments.					
34189908	2	55	theme	glycoproteins	376:388	arg1	preparation					349:359	the preparation	345:359	the preparation of homogeneous glycoproteins	345:388	To understand the biological processes arising from each glycoform, the preparation of homogeneous glycoproteins is essential for extensive biological experiments.					
34189908	0	56	theme	Glycosyl	52:59	arg1	Asparagine					61:70	Glycosyl Asparagine	52:70	Glycosyl Asparagine	52:70	Glycoprotein Semisynthesis by Chemical Insertion of Glycosyl Asparagine Using a Bifunctional Thioacid-Mediated Strategy.					
34189908	2	57	gly	glycoproteins	376:388	arg1	glycoproteins					376:388	homogeneous glycoproteins	364:388	homogeneous glycoproteins	364:388	To understand the biological processes arising from each glycoform, the preparation of homogeneous glycoproteins is essential for extensive biological experiments.					
34189908	5	58	dep	N-	916:917	arg1	the					912:914	the	912:914	the	912:914	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	1	59	theme	major	140:144	arg1	modification					146:157	a major modification	138:157	a major modification of secreted and cell surface proteins	138:195	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	1	59	theme	major	140:144	arg1	Glycosylation					121:133	Glycosylation	121:133	Glycosylation	121:133	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	7	60	theme	glycosyl	1339:1346	arg1	thioacid					1359:1366	glycosyl asparagine thioacid	1339:1366	glycosyl asparagine thioacid	1339:1366	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	1	61	from	heterogeneity	242:254	arg1	structures					265:274	their structures	259:274	their structures	259:274	Glycosylation is a major modification of secreted and cell surface proteins, and the resultant glycans show considerable heterogeneity in their structures.					
34189908	4	62	theme	yielded	709:715	arg1	glycopeptides					717:729	yielded glycopeptides	709:729	yielded glycopeptides	709:729	We found that diacyl disulfide coupling (DDC) formed with glycosyl asparagine thioacid and peptide thioacid yielded glycopeptides.					
34189908	3	63	theme	homogeneous	515:525	arg1	glycoproteins					527:539	homogeneous glycoproteins	515:539	homogeneous glycoproteins	515:539	To establish a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins, we studied several key reactions based on amino thioacids.					
34189908	5	64	gly	glycoprotein	793:804	arg1	glycoprotein					793:804	a new glycoprotein synthesis method	787:821	a new glycoprotein synthesis method	787:821	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	5	65	theme	coupling	747:754	arg1	reaction					756:763	This efficient coupling reaction	732:763	This efficient coupling reaction	732:763	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	7	66	theme	amino	1294:1298	arg1	thioacids					1300:1308	amino thioacids	1294:1308	amino thioacids	1294:1308	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	3	67	theme	glycoproteins	527:539	arg1	synthesis					502:510	the synthesis	498:510	the synthesis of homogeneous glycoproteins	498:539	To establish a more robust and rapid synthetic route for the synthesis of homogeneous glycoproteins, we studied several key reactions based on amino thioacids.					
34189908	7	68	theme	asparagine	1348:1357	arg1	thioacid					1359:1366	glycosyl asparagine thioacid	1339:1366	glycosyl asparagine thioacid	1339:1366	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	5	69	theme	synthesis	806:814	arg1	method					816:821	a new glycoprotein synthesis method	787:821	a new glycoprotein synthesis method	787:821	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	4	70	theme	asparagine	668:677	arg1	thioacid					679:686	glycosyl asparagine thioacid	659:686	glycosyl asparagine thioacid	659:686	We found that diacyl disulfide coupling (DDC) formed with glycosyl asparagine thioacid and peptide thioacid yielded glycopeptides.					
34189908	2	71	theme	extensive	407:415	arg1	experiments					428:438	extensive biological experiments	407:438	extensive biological experiments	407:438	To understand the biological processes arising from each glycoform, the preparation of homogeneous glycoproteins is essential for extensive biological experiments.					
34189908	7	72	theme	robust	1383:1388	arg1	semisynthesis					1403:1415	robust glycoprotein semisynthesis	1383:1415	robust glycoprotein semisynthesis	1383:1415	Herein, we report the characterization of the DDC of amino thioacids and the efficient ability of glycosyl asparagine thioacid to be used for robust glycoprotein semisynthesis.					
34189908	5	73	theme	bifunctional	836:847	arg1	strategy					867:874	the bifunctional thioacid-mediated strategy	832:874	the bifunctional thioacid-mediated strategy	832:874	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	6	74	theme	synthesis	988:996	arg1	methods					998:1004	Previous glycoprotein synthesis methods	966:1004	Previous glycoprotein synthesis methods	966:1004	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	6	75	gly	glycoprotein	1087:1098	arg1	glycoprotein					1087:1098	subsequent multiple glycoprotein synthesis	1067:1108	subsequent multiple glycoprotein synthesis	1067:1108	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	5	76	theme	glycosyl	936:943	arg1	thioacid					956:963	glycosyl asparagine thioacid	936:963	glycosyl asparagine thioacid	936:963	This efficient coupling reaction enabled us to develop a new glycoprotein synthesis method, such as the bifunctional thioacid-mediated strategy, which can couple two peptides with the N- and C-termini of glycosyl asparagine thioacid.					
34189908	6	77	gly	glycoprotein	975:986	arg1	glycoprotein					975:986	Previous glycoprotein synthesis methods	966:1004	Previous glycoprotein synthesis methods	966:1004	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
34189908	6	78	theme	glycoprotein	1087:1098	arg1	synthesis					1100:1108	subsequent multiple glycoprotein synthesis	1067:1108	subsequent multiple glycoprotein synthesis	1067:1108	Previous glycoprotein synthesis methods required valuable glycosyl asparagine in the early stage and subsequent multiple glycoprotein synthesis routes, whereas the developed concept can generate glycoproteins within a few steps from peptide and glycosyl asparagine thioacid.					
32106961	2	0	theme	mass	549:552	arg1	spectrometry					554:565	mass spectrometry	549:565	mass spectrometry	549:565	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	3	1	from	samples	733:739	arg1	glycans					710:716	neutral glycans	702:716	neutral glycans from different samples	702:739	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	2	2	theme	solid-phase	386:396	arg1	extraction					398:407	fluorous solid-phase extraction	377:407	fluorous solid-phase extraction	377:407	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	1	3	theme	N-glycans	277:285	arg1	analysis					265:272	comparative analysis	253:272	comparative analysis of N-glycans	253:285	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	7	4	theme	early	1534:1538	arg1	diagnosis					1540:1548	GC early diagnosis	1531:1548	GC early diagnosis	1531:1548	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	5	5	theme	dynamic	1184:1190	arg1	range					1192:1196	the dynamic range	1180:1196	the dynamic range	1180:1196	This method provided a good linearity (R2 > 0.99) and high reproducibility (CV < 20%) within 2 orders of magnitude in the dynamic range.					
32106961	6	6	theme	gastric	1312:1318	arg1	cancer					1320:1325	gastric cancer	1312:1325	gastric cancer (GC)	1312:1330	Finally, this strategy was successfully applied to investigate the N-glycome alteration in serum associated with gastric cancer (GC).					
32106961	6	6	theme	gastric	1312:1318	arg1	GC					1328:1329	GC	1328:1329	GC	1328:1329	Finally, this strategy was successfully applied to investigate the N-glycome alteration in serum associated with gastric cancer (GC).					
32106961	2	7	theme	fluorous	377:384	arg1	extraction					398:407	fluorous solid-phase extraction	377:407	fluorous solid-phase extraction	377:407	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	0	8	theme	N-glycans	118:126	arg1	discovery					86:94	simultaneous discovery	73:94	simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer	73:159	Dual isotopic labeling combined with fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer.					
32106961	3	9	theme	mass	678:681	arg1	isotopic					572:579	isotopic	572:579	isotopic	572:579	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	3	9	theme	mass	678:681	arg1	difference					683:692	mass difference	678:692	mass difference between neutral glycans from different samples	678:739	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	3	9	theme	mass	678:681	arg1	tag					661:663	an enrichment tag	647:663	an enrichment tag	647:663	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	1	10	theme	glycan	291:296	arg1	discovery					308:316	glycan biomarker discovery	291:316	glycan biomarker discovery	291:316	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	0	11	theme	neutral/sialylated	99:116	arg1	N-glycans					118:126	neutral/sialylated N-glycans	99:126	neutral/sialylated N-glycans	99:126	Dual isotopic labeling combined with fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer.					
32106961	3	12	theme	non-reducing	774:785	arg1	end					787:789	the non-reducing end	770:789	the non-reducing end of glycans	770:800	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	7	13	theme	great	1488:1492	arg1	potential					1494:1502	the great potential	1484:1502	the great potential to be novel biomarkers for GC early diagnosis	1484:1548	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	1	14	theme	biomarker	298:306	arg1	discovery					308:316	glycan biomarker discovery	291:316	glycan biomarker discovery	291:316	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	7	15	theme	novel	1510:1514	arg1	biomarkers					1516:1525	novel biomarkers	1510:1525	novel biomarkers for GC early diagnosis	1510:1548	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	7	16	theme	glycan	1367:1372	arg1	compositions					1374:1385	Bisecting GlcNAc and triantennary glycan compositions	1333:1385	Bisecting GlcNAc and triantennary glycan compositions	1333:1385	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	3	17	theme	fluorous	590:597	arg1	compound					599:606	fluorous compound	590:606	fluorous compound	590:606	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	2	18	theme	selective	433:441	arg1	enrichment					443:452	selective enrichment	433:452	selective enrichment	433:452	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	2	19	theme	glycans	519:525	arg1	recognition					485:495	recognition	485:495	recognition	485:495	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	2	19	theme	glycans	519:525	arg1	enrichment					443:452	selective enrichment	433:452	selective enrichment	433:452	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	2	19	theme	glycans	519:525	arg1	quantitation					468:479	simultaneous quantitation	455:479	simultaneous quantitation	455:479	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	2	20	from	samples	538:544	arg1	quantitation					468:479	simultaneous quantitation	455:479	simultaneous quantitation	455:479	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	2	20	from	samples	538:544	arg1	glycans					519:525	neutral/sialylated glycans	500:525	neutral/sialylated glycans from serum samples	500:544	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	2	20	from	samples	538:544	arg1	enrichment					443:452	selective enrichment	433:452	selective enrichment	433:452	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	2	20	from	samples	538:544	arg1	recognition					485:495	recognition	485:495	recognition	485:495	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	2	21	theme	neutral/sialylated	500:517	arg1	glycans					519:525	neutral/sialylated glycans	500:525	neutral/sialylated glycans from serum samples	500:544	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	1	22	theme	stable	202:207	arg1	labeling					217:224	stable isotope labeling	202:224	stable isotope labeling	202:224	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	0	23	theme	isotopic	5:12	arg1	labeling					14:21	Dual isotopic labeling	0:21	Dual isotopic labeling	0:21	Dual isotopic labeling combined with fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer.					
32106961	5	24	theme	magnitude	1167:1175	arg1	orders					1157:1162	2 orders	1155:1162	2 orders of magnitude	1155:1175	This method provided a good linearity (R2 > 0.99) and high reproducibility (CV < 20%) within 2 orders of magnitude in the dynamic range.					
32106961	4	25	theme	neutral/sialylated	914:931	arg1	glycans					933:939	the neutral/sialylated glycans	910:939	the neutral/sialylated glycans	910:939	Therefore, the neutral/sialylated glycans could be simultaneously enriched through the fluorous solid-phase extraction (FSPE) and quantified by mass spectrometry.					
32106961	0	26	gly	neutral/sialylated	99:116	arg1	N-glycans					118:126	neutral/sialylated N-glycans	99:126	neutral/sialylated N-glycans	99:126	Dual isotopic labeling combined with fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer.					
32106961	3	27	theme	sialylated	879:888	arg1	glycans					890:896	sialylated glycans	879:896	sialylated glycans	879:896	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	1	28	theme	isotope	209:215	arg1	labeling					217:224	stable isotope labeling	202:224	stable isotope labeling	202:224	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	0	29	theme	Dual	0:3	arg1	labeling					14:21	Dual isotopic labeling	0:21	Dual isotopic labeling	0:21	Dual isotopic labeling combined with fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer.					
32106961	3	30	theme	glycans	631:637	arg1	end					624:626	the reducing end	611:626	the reducing end of glycans	611:637	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	0	31	theme	gastric	146:152	arg1	cancer					154:159	gastric cancer	146:159	gastric cancer	146:159	Dual isotopic labeling combined with fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer.					
32106961	2	32	theme	novel	327:331	arg1	method					333:338	a novel method	325:338	a novel method combining dual isotopic labeling and fluorous solid-phase extraction	325:407	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	7	33	theme	triantennary	1354:1365	arg1	compositions					1374:1385	Bisecting GlcNAc and triantennary glycan compositions	1333:1385	Bisecting GlcNAc and triantennary glycan compositions	1333:1385	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	3	34	dep	isotopic	572:579	arg1	label					581:585	label	581:585	label	581:585	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	3	35	dep	isotopic	752:759	arg1	label					761:765	label	761:765	label on the non-reducing end of glycans	761:800	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	4	36	theme	solid-phase	995:1005	arg1	extraction					1007:1016	the fluorous solid-phase extraction	982:1016	the fluorous solid-phase extraction (FSPE)	982:1023	Therefore, the neutral/sialylated glycans could be simultaneously enriched through the fluorous solid-phase extraction (FSPE) and quantified by mass spectrometry.					
32106961	4	36	theme	solid-phase	995:1005	arg1	FSPE					1019:1022	FSPE	1019:1022	FSPE	1019:1022	Therefore, the neutral/sialylated glycans could be simultaneously enriched through the fluorous solid-phase extraction (FSPE) and quantified by mass spectrometry.					
32106961	3	37	theme	neutral	702:708	arg1	glycans					710:716	neutral glycans	702:716	neutral glycans from different samples	702:739	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	7	38	theme	healthy	1456:1462	arg1	control					1464:1470	healthy control	1456:1470	healthy control	1456:1470	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	2	39	theme	simultaneous	455:466	arg1	quantitation					468:479	simultaneous quantitation	455:479	simultaneous quantitation	455:479	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	0	40	theme	fluorous	37:44	arg1	extraction					58:67	fluorous solid-phase extraction	37:67	fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer	37:159	Dual isotopic labeling combined with fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer.					
32106961	3	41	theme	glycans	794:800	arg1	end					787:789	the non-reducing end	770:789	the non-reducing end of glycans	770:800	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	7	42	theme	GlcNAc	1343:1348	arg1	compositions					1374:1385	Bisecting GlcNAc and triantennary glycan compositions	1333:1385	Bisecting GlcNAc and triantennary glycan compositions	1333:1385	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	1	43	theme	powerful	231:238	arg1	analysis					180:187	Mass spectrometry analysis	162:187	Mass spectrometry analysis coupled with stable isotope labeling	162:224	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	1	43	theme	powerful	231:238	arg1	strategy					240:247	a powerful strategy	229:247	a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery	229:316	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	2	44	theme	serum	532:536	arg1	samples					538:544	serum samples	532:544	serum samples	532:544	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	3	45	gly	sialylated	879:888	arg1	glycans					890:896	sialylated glycans	879:896	sialylated glycans	879:896	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	3	46	theme	enrichment	650:659	arg1	isotopic					572:579	isotopic	572:579	isotopic	572:579	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	3	46	theme	enrichment	650:659	arg1	difference					683:692	mass difference	678:692	mass difference between neutral glycans from different samples	678:739	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	3	46	theme	enrichment	650:659	arg1	tag					661:663	an enrichment tag	647:663	an enrichment tag	647:663	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	4	47	gly	neutral/sialylated	914:931	arg1	glycans					933:939	the neutral/sialylated glycans	910:939	the neutral/sialylated glycans	910:939	Therefore, the neutral/sialylated glycans could be simultaneously enriched through the fluorous solid-phase extraction (FSPE) and quantified by mass spectrometry.					
32106961	7	48	theme	GC	1434:1435	arg1	cases					1437:1441	GC cases	1434:1441	GC cases (n = 50)	1434:1450	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	7	48	theme	GC	1434:1435	arg1	n = 50					1444:1449	n = 50	1444:1449	n = 50	1444:1449	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	0	49	theme	solid-phase	46:56	arg1	extraction					58:67	fluorous solid-phase extraction	37:67	fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer	37:159	Dual isotopic labeling combined with fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer.					
32106961	3	50	theme	sialic	815:820	arg1	residue					827:833	the sialic acid residue	811:833	the sialic acid residue	811:833	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	7	51	theme	GC	1531:1532	arg1	diagnosis					1540:1548	GC early diagnosis	1531:1548	GC early diagnosis	1531:1548	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	7	52	theme	Bisecting	1333:1341	arg1	GlcNAc					1343:1348	Bisecting GlcNAc	1333:1348	Bisecting GlcNAc	1333:1348	Bisecting GlcNAc and triantennary glycan compositions were found to be significantly changed between GC cases (n = 50) and healthy control, indicating the great potential to be novel biomarkers for GC early diagnosis.					
32106961	3	53	theme	acid	822:825	arg1	residue					827:833	the sialic acid residue	811:833	the sialic acid residue	811:833	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	4	54	theme	fluorous	986:993	arg1	extraction					1007:1016	the fluorous solid-phase extraction	982:1016	the fluorous solid-phase extraction (FSPE)	982:1023	Therefore, the neutral/sialylated glycans could be simultaneously enriched through the fluorous solid-phase extraction (FSPE) and quantified by mass spectrometry.					
32106961	4	54	theme	fluorous	986:993	arg1	FSPE					1019:1022	FSPE	1019:1022	FSPE	1019:1022	Therefore, the neutral/sialylated glycans could be simultaneously enriched through the fluorous solid-phase extraction (FSPE) and quantified by mass spectrometry.					
32106961	5	55	theme	high	1116:1119	arg1	reproducibility					1121:1135	high reproducibility	1116:1135	high reproducibility (CV < 20%)	1116:1146	This method provided a good linearity (R2 > 0.99) and high reproducibility (CV < 20%) within 2 orders of magnitude in the dynamic range.					
32106961	5	55	theme	high	1116:1119	arg1	%					1145:1145	CV < 20%	1138:1145	CV < 20%	1138:1145	This method provided a good linearity (R2 > 0.99) and high reproducibility (CV < 20%) within 2 orders of magnitude in the dynamic range.					
32106961	6	56	theme	N-glycome	1266:1274	arg1	alteration					1276:1285	the N-glycome alteration	1262:1285	the N-glycome alteration in serum associated with gastric cancer (GC)	1262:1330	Finally, this strategy was successfully applied to investigate the N-glycome alteration in serum associated with gastric cancer (GC).					
32106961	2	57	theme	isotopic	355:362	arg1	labeling					364:371	dual isotopic labeling	350:371	dual isotopic labeling	350:371	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	3	58	theme	reducing	615:622	arg1	end					624:626	the reducing end	611:626	the reducing end of glycans	611:637	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	3	59	theme	mass	859:862	arg1	difference					864:873	additional mass difference	848:873	additional mass difference for sialylated glycans	848:896	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	3	60	theme	compound	599:606	arg1	isotopic					572:579	isotopic	572:579	isotopic	572:579	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	3	60	theme	compound	599:606	arg1	difference					683:692	mass difference	678:692	mass difference between neutral glycans from different samples	678:739	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	3	60	theme	compound	599:606	arg1	tag					661:663	an enrichment tag	647:663	an enrichment tag	647:663	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	2	61	gly	neutral/sialylated	500:517	arg1	glycans					519:525	neutral/sialylated glycans	500:525	neutral/sialylated glycans from serum samples	500:544	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	2	62	theme	dual	350:353	arg1	labeling					364:371	dual isotopic labeling	350:371	dual isotopic labeling	350:371	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	1	63	theme	comparative	253:263	arg1	analysis					265:272	comparative analysis	253:272	comparative analysis of N-glycans	253:285	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	0	64	theme	simultaneous	73:84	arg1	discovery					86:94	simultaneous discovery	73:94	simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer	73:159	Dual isotopic labeling combined with fluorous solid-phase extraction for simultaneous discovery of neutral/sialylated N-glycans as biomarkers for gastric cancer.					
32106961	3	65	theme	different	723:731	arg1	samples					733:739	different samples	723:739	different samples	723:739	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
32106961	2	66	from	enrichment	443:452	arg1	samples					538:544	serum samples	532:544	serum samples	532:544	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	1	67	theme	Mass	162:165	arg1	analysis					180:187	Mass spectrometry analysis	162:187	Mass spectrometry analysis coupled with stable isotope labeling	162:224	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	1	67	theme	Mass	162:165	arg1	strategy					240:247	a powerful strategy	229:247	a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery	229:316	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	6	68	from	alteration	1276:1285	arg1	serum					1290:1294	serum	1290:1294	serum associated with gastric cancer (GC)	1290:1330	Finally, this strategy was successfully applied to investigate the N-glycome alteration in serum associated with gastric cancer (GC).					
32106961	4	69	theme	mass	1043:1046	arg1	spectrometry					1048:1059	mass spectrometry	1043:1059	mass spectrometry	1043:1059	Therefore, the neutral/sialylated glycans could be simultaneously enriched through the fluorous solid-phase extraction (FSPE) and quantified by mass spectrometry.					
32106961	2	70	from	recognition	485:495	arg1	samples					538:544	serum samples	532:544	serum samples	532:544	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	5	71	theme	good	1085:1088	arg1	R2 > 0.99					1101:1109	R2 > 0.99	1101:1109	R2 > 0.99	1101:1109	This method provided a good linearity (R2 > 0.99) and high reproducibility (CV < 20%) within 2 orders of magnitude in the dynamic range.					
32106961	5	71	theme	good	1085:1088	arg1	linearity					1090:1098	a good linearity	1083:1098	a good linearity (R2 > 0.99)	1083:1110	This method provided a good linearity (R2 > 0.99) and high reproducibility (CV < 20%) within 2 orders of magnitude in the dynamic range.					
32106961	2	72	from	quantitation	468:479	arg1	samples					538:544	serum samples	532:544	serum samples	532:544	Here, a novel method combining dual isotopic labeling and fluorous solid-phase extraction was developed, enabling selective enrichment, simultaneous quantitation and recognition of neutral/sialylated glycans from serum samples by mass spectrometry.					
32106961	1	73	theme	spectrometry	167:178	arg1	analysis					180:187	Mass spectrometry analysis	162:187	Mass spectrometry analysis coupled with stable isotope labeling	162:224	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	1	73	theme	spectrometry	167:178	arg1	strategy					240:247	a powerful strategy	229:247	a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery	229:316	Mass spectrometry analysis coupled with stable isotope labeling is a powerful strategy for comparative analysis of N-glycans for glycan biomarker discovery.					
32106961	3	74	theme	additional	848:857	arg1	difference					864:873	additional mass difference	848:873	additional mass difference for sialylated glycans	848:896	The isotopic label of fluorous compound on the reducing end of glycans acts as an enrichment tag and provides mass difference between neutral glycans from different samples, while the isotopic label on the non-reducing end of glycans protects the sialic acid residue and provides additional mass difference for sialylated glycans.					
33300874	7	0	theme	direct	1256:1261	arg1	contact					1271:1277	direct surface contact	1256:1277	direct surface contact	1256:1277	Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.					
33300874	7	1	theme	adhering	1156:1163	arg1	cells					1180:1184	adhering C. reinhardtii cells	1156:1184	adhering C. reinhardtii cells onto surfaces	1156:1198	Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.					
33300874	3	2	theme	insertional	505:515	arg1	mutants					517:523	several C. reinhardtii insertional mutants	482:523	several C. reinhardtii insertional mutants	482:523	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	7	3	theme	surface	1263:1269	arg1	contact					1271:1277	direct surface contact	1256:1277	direct surface contact	1256:1277	Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.					
33300874	7	4	theme	C.	1165:1166	arg1	cells					1180:1184	adhering C. reinhardtii cells	1156:1184	adhering C. reinhardtii cells onto surfaces	1156:1198	Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.					
33300874	5	5	theme	solid	934:938	arg1	surfaces					940:947	solid surfaces	934:947	solid surfaces	934:947	This results in impaired polystyrene bead binding and transport but not gliding of cells on solid surfaces.					
33300874	3	6	contain	possessing	589:598	arg1	mutant					552:557	a CRISPR/Cas9 knockout mutant	529:557	a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A	529:582	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	3	6	contain	possessing	589:598	arg2	compositions					617:628	altered N-glycan compositions	600:628	altered N-glycan compositions	600:628	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	3	6	contain	possessing	589:598	arg1	all					585:587	all	585:587	all	585:587	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	1	7	attach	presence	150:157	arg2	proteins					177:184	N-glycosylated proteins	162:184	N-glycosylated proteins	162:184	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	1	7	attach	presence	150:157	arg1	surface					193:199	the surface	189:199	the surface of two flagella	189:215	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	5	8	theme	cells	925:929	arg1	gliding					914:920	gliding	914:920	gliding of cells on solid surfaces	914:947	This results in impaired polystyrene bead binding and transport but not gliding of cells on solid surfaces.					
33300874	4	9	theme	adhesion	780:787	arg1	force					789:793	the adhesion force	776:793	the adhesion force required for binding the flagella to surfaces	776:839	Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces.					
33300874	1	10	theme	surface	287:293	arg1	adhesion					295:302	flagellar surface adhesion	277:302	flagellar surface adhesion	277:302	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	4	11	theme	N-glycan	748:755	arg1	complexity					757:766	N-glycan complexity	748:766	N-glycan complexity	748:766	Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces.					
33300874	7	12	theme	proteins	1132:1139	arg1	N-glycosylation					1103:1117	proper N-glycosylation	1096:1117	proper N-glycosylation of flagellar proteins	1096:1139	Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.					
33300874	5	13	theme	bead	879:882	arg1	binding					884:890	impaired polystyrene bead binding	858:890	impaired polystyrene bead binding	858:890	This results in impaired polystyrene bead binding and transport but not gliding of cells on solid surfaces.					
33300874	3	14	theme	CRISPR/Cas9	531:541	arg1	mutant					552:557	a CRISPR/Cas9 knockout mutant	529:557	a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A	529:582	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	3	14	theme	CRISPR/Cas9	531:541	arg1	all					585:587	all	585:587	all	585:587	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	2	15	theme	respective	405:414	arg1	proteins					416:423	respective proteins	405:423	respective proteins	405:423	However, it is not known whether only the presence or also the composition of N-glycans attached to respective proteins is important for these processes.					
33300874	7	16	theme	surface	1235:1241	arg1	adhesion					1243:1250	surface adhesion	1235:1250	surface adhesion	1235:1250	Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.					
33300874	1	17	from	presence	150:157	arg1	surface					193:199	the surface	189:199	the surface of two flagella	189:215	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	3	18	theme	knockout	543:550	arg1	mutant					552:557	a CRISPR/Cas9 knockout mutant	529:557	a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A	529:582	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	3	18	theme	knockout	543:550	arg1	all					585:587	all	585:587	all	585:587	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	4	19	theme	micropipette	679:690	arg1	measurements					698:709	micropipette force measurements	679:709	micropipette force measurements	679:709	Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces.					
33300874	0	20	theme	N-glycan	8:15	arg1	composition					17:27	Altered N-glycan composition	0:27	Altered N-glycan composition	0:27	Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii.					
33300874	1	21	theme	unicellular	102:112	arg1	reinhardtii					133:143	the unicellular alga Chlamydomonas reinhardtii	98:143	the unicellular alga Chlamydomonas reinhardtii	98:143	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	0	22	theme	Altered	0:6	arg1	composition					17:27	Altered N-glycan composition	0:27	Altered N-glycan composition	0:27	Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii.					
33300874	7	23	theme	flagellar	1122:1130	arg1	proteins					1132:1139	flagellar proteins	1122:1139	flagellar proteins	1122:1139	Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.					
33300874	4	24	theme	force	692:696	arg1	measurements					698:709	micropipette force measurements	679:709	micropipette force measurements	679:709	Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces.					
33300874	1	25	theme	alga	114:117	arg1	reinhardtii					133:143	the unicellular alga Chlamydomonas reinhardtii	98:143	the unicellular alga Chlamydomonas reinhardtii	98:143	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	2	26	theme	N-glycans	383:391	arg1	presence					347:354	the presence	343:354	the presence	343:354	However, it is not known whether only the presence or also the composition of N-glycans attached to respective proteins is important for these processes.					
33300874	2	26	theme	N-glycans	383:391	arg1	important					428:436	important	428:436	important	428:436	However, it is not known whether only the presence or also the composition of N-glycans attached to respective proteins is important for these processes.					
33300874	2	26	theme	N-glycans	383:391	arg1	composition					368:378	the composition	364:378	the composition of N-glycans attached to respective proteins	364:423	However, it is not known whether only the presence or also the composition of N-glycans attached to respective proteins is important for these processes.					
33300874	6	27	theme	protein	999:1005	arg1	import					1007:1012	protein import	999:1012	protein import	999:1012	Notably, assembly, intraflagellar transport, and protein import into flagella are not affected by altered N-glycosylation.					
33300874	7	28	theme	proper	1096:1101	arg1	N-glycosylation					1103:1117	proper N-glycosylation	1096:1117	proper N-glycosylation of flagellar proteins	1096:1139	Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.					
33300874	1	29	theme	Chlamydomonas	119:131	arg1	reinhardtii					133:143	the unicellular alga Chlamydomonas reinhardtii	98:143	the unicellular alga Chlamydomonas reinhardtii	98:143	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	4	30	theme	force	658:662	arg1	microscopy					664:673	atomic force microscopy	651:673	atomic force microscopy	651:673	Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces.					
33300874	5	31	theme	impaired	858:865	arg1	binding					884:890	impaired polystyrene bead binding	858:890	impaired polystyrene bead binding	858:890	This results in impaired polystyrene bead binding and transport but not gliding of cells on solid surfaces.					
33300874	3	32	theme	1A	581:582	arg1	mutant					552:557	a CRISPR/Cas9 knockout mutant	529:557	a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A	529:582	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	3	32	theme	1A	581:582	arg1	mutants					517:523	several C. reinhardtii insertional mutants	482:523	several C. reinhardtii insertional mutants	482:523	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	3	32	theme	1A	581:582	arg1	all					585:587	all	585:587	all	585:587	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	4	33	theme	atomic	651:656	arg1	microscopy					664:673	atomic force microscopy	651:673	atomic force microscopy	651:673	Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces.					
33300874	3	34	theme	N-glycan	608:615	arg1	compositions					617:628	altered N-glycan compositions	600:628	altered N-glycan compositions	600:628	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	1	35	theme	flagella	208:215	arg1	surface					193:199	the surface	189:199	the surface of two flagella	189:215	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	0	36	theme	flagella-mediated	37:53	arg1	adhesion					55:62	flagella-mediated adhesion	37:62	flagella-mediated adhesion	37:62	Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii.					
33300874	1	37	theme	flagellar	277:285	arg1	adhesion					295:302	flagellar surface adhesion	277:302	flagellar surface adhesion	277:302	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	4	38	theme	microscopy	664:673	arg1	advantage					638:646	advantage	638:646	advantage of atomic force microscopy and micropipette force measurements	638:709	Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces.					
33300874	7	39	theme	reinhardtii	1168:1178	arg1	cells					1180:1184	adhering C. reinhardtii cells	1156:1184	adhering C. reinhardtii cells onto surfaces	1156:1198	Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.					
33300874	6	40	theme	intraflagellar	969:982	arg1	transport					984:992	intraflagellar transport	969:992	intraflagellar transport	969:992	Notably, assembly, intraflagellar transport, and protein import into flagella are not affected by altered N-glycosylation.					
33300874	5	41	from	gliding	914:920	arg1	surfaces					940:947	solid surfaces	934:947	solid surfaces	934:947	This results in impaired polystyrene bead binding and transport but not gliding of cells on solid surfaces.					
33300874	3	42	theme	C.	490:491	arg1	mutants					517:523	several C. reinhardtii insertional mutants	482:523	several C. reinhardtii insertional mutants	482:523	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	7	43	gly	N-glycosylation	1103:1117	arg1	proteins					1132:1139	flagellar proteins	1122:1139	flagellar proteins	1122:1139	Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.					
33300874	1	44	gly	N-glycosylated	162:175	arg1	proteins					177:184	N-glycosylated proteins	162:184	N-glycosylated proteins	162:184	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	3	45	theme	altered	600:606	arg1	compositions					617:628	altered N-glycan compositions	600:628	altered N-glycan compositions	600:628	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	3	46	theme	reinhardtii	493:503	arg1	mutants					517:523	several C. reinhardtii insertional mutants	482:523	several C. reinhardtii insertional mutants	482:523	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	4	47	theme	measurements	698:709	arg1	advantage					638:646	advantage	638:646	advantage of atomic force microscopy and micropipette force measurements	638:709	Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces.					
33300874	3	48	theme	xylosyltransferase	562:579	arg1	1A					581:582	xylosyltransferase 1A	562:582	xylosyltransferase 1A	562:582	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	1	49	theme	cell-cell	237:245	arg1	interaction					247:257	cell-cell interaction	237:257	cell-cell interaction during mating	237:271	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	3	50	theme	several	482:488	arg1	mutants					517:523	several C. reinhardtii insertional mutants	482:523	several C. reinhardtii insertional mutants	482:523	To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions.					
33300874	6	51	theme	altered	1048:1054	arg1	N-glycosylation					1056:1070	altered N-glycosylation	1048:1070	altered N-glycosylation	1048:1070	Notably, assembly, intraflagellar transport, and protein import into flagella are not affected by altered N-glycosylation.					
33300874	2	52	attach	attached	393:400	arg2	N-glycans					383:391	N-glycans	383:391	N-glycans attached to respective proteins	383:423	However, it is not known whether only the presence or also the composition of N-glycans attached to respective proteins is important for these processes.					
33300874	2	52	attach	attached	393:400	arg1	proteins					416:423	respective proteins	405:423	respective proteins	405:423	However, it is not known whether only the presence or also the composition of N-glycans attached to respective proteins is important for these processes.					
33300874	5	53	theme	polystyrene	867:877	arg1	binding					884:890	impaired polystyrene bead binding	858:890	impaired polystyrene bead binding	858:890	This results in impaired polystyrene bead binding and transport but not gliding of cells on solid surfaces.					
33300874	1	54	theme	N-glycosylated	162:175	arg1	proteins					177:184	N-glycosylated proteins	162:184	N-glycosylated proteins	162:184	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	4	55	from	reduction	735:743	arg1	complexity					757:766	N-glycan complexity	748:766	N-glycan complexity	748:766	Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces.					
33300874	1	56	theme	proteins	177:184	arg1	presence					150:157	the presence	146:157	the presence of N-glycosylated proteins on the surface of two flagella	146:215	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
33300874	1	56	theme	proteins	177:184	arg1	crucial					220:226	crucial	220:226	crucial	220:226	For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion.					
34335667	0	0	theme	Arabidopsis	92:102	arg1	Culture					109:115	Glycoengineered Arabidopsis Cell Culture	76:115	Glycoengineered Arabidopsis Cell Culture	76:115	Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture.					
34335667	0	1	with	Glucosidase	31:41	arg1	Structure					63:71	a Paucimannose Structure	48:71	a Paucimannose Structure	48:71	Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture.					
34335667	1	2	theme	promising	156:164	arg1	platform					166:173	a promising platform	154:173	a promising platform for the production of biopharmaceutics due to their cost-effectiveness, safety, ability to control the cultivation, and secrete products into culture medium	154:330	Plant cell cultures have emerged as a promising platform for the production of biopharmaceutics due to their cost-effectiveness, safety, ability to control the cultivation, and secrete products into culture medium.					
34335667	6	3	theme	NaCl-supplemented	996:1012	arg1	culture					1014:1020	an NaCl-supplemented culture	993:1020	an NaCl-supplemented culture	993:1020	GAA from an NaCl-supplemented culture showed a similar N-glycan profile, indicating that the NaCl supplementation did not affect N-glycosylation.					
34335667	0	4	theme	Glycoengineered	76:90	arg1	Culture					109:115	Glycoengineered Arabidopsis Cell Culture	76:115	Glycoengineered Arabidopsis Cell Culture	76:115	Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture.					
34335667	3	5	theme	Arabidopsis	677:687	arg1	culture					699:705	a glycoengineered Arabidopsis alg3 cell culture	659:705	a glycoengineered Arabidopsis alg3 cell culture	659:705	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	6	6	from	culture	1014:1020	arg1	GAA					984:986	GAA	984:986	GAA from an NaCl-supplemented culture	984:1020	GAA from an NaCl-supplemented culture showed a similar N-glycan profile, indicating that the NaCl supplementation did not affect N-glycosylation.					
34335667	2	7	theme	productivity	522:533	arg1	generation					386:395	the generation	382:395	the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity	382:533	However, the use of this platform is hindered by the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity.					
34335667	3	8	theme	alg3	689:692	arg1	culture					699:705	a glycoengineered Arabidopsis alg3 cell culture	659:705	a glycoengineered Arabidopsis alg3 cell culture	659:705	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	4	9	theme	GAA	740:742	arg1	composition					721:731	The N-glycan composition	708:731	The N-glycan composition of the GAA	708:742	The N-glycan composition of the GAA consisted of a predominantly paucimannosidic structure, Man3GlcNAc2 (M3), without the plant-specific N-glycans.					
34335667	0	10	theme	Cell	104:107	arg1	Culture					109:115	Glycoengineered Arabidopsis Cell Culture	76:115	Glycoengineered Arabidopsis Cell Culture	76:115	Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture.					
34335667	7	11	dep	receptor-mediated	1284:1300	arg1	M3					1270:1271	M3	1270:1271	M3	1270:1271	The results of this study highlight the feasibility of using a glycoengineered plant cell culture to produce recombinant proteins for which M3 or mannose receptor-mediated delivery is desired.					
34335667	7	12	theme	receptor-mediated	1284:1300	arg1	delivery					1302:1309	M3 or mannose receptor-mediated delivery	1270:1309	M3 or mannose receptor-mediated delivery	1270:1309	The results of this study highlight the feasibility of using a glycoengineered plant cell culture to produce recombinant proteins for which M3 or mannose receptor-mediated delivery is desired.					
34335667	7	13	theme	glycoengineered	1193:1207	arg1	culture					1220:1226	a glycoengineered plant cell culture	1191:1226	a glycoengineered plant cell culture	1191:1226	The results of this study highlight the feasibility of using a glycoengineered plant cell culture to produce recombinant proteins for which M3 or mannose receptor-mediated delivery is desired.					
34335667	5	14	theme	final	904:908	arg1	concentration					910:922	a final concentration	902:922	a final concentration of 50 mM	902:931	Supplementing the culture medium with NaCl to a final concentration of 50 mM successfully increased GAA production by 3.8-fold.					
34335667	4	15	theme	plant-specific	830:843	arg1	N-glycans					845:853	the plant-specific N-glycans	826:853	the plant-specific N-glycans	826:853	The N-glycan composition of the GAA consisted of a predominantly paucimannosidic structure, Man3GlcNAc2 (M3), without the plant-specific N-glycans.					
34335667	4	16	theme	paucimannosidic	773:787	arg1	Man3GlcNAc2					800:810	Man3GlcNAc2	800:810	Man3GlcNAc2 (M3)	800:815	The N-glycan composition of the GAA consisted of a predominantly paucimannosidic structure, Man3GlcNAc2 (M3), without the plant-specific N-glycans.					
34335667	4	16	theme	paucimannosidic	773:787	arg1	structure					789:797	a predominantly paucimannosidic structure	757:797	a predominantly paucimannosidic structure	757:797	The N-glycan composition of the GAA consisted of a predominantly paucimannosidic structure, Man3GlcNAc2 (M3), without the plant-specific N-glycans.					
34335667	7	17	theme	plant	1209:1213	arg1	culture					1220:1226	a glycoengineered plant cell culture	1191:1226	a glycoengineered plant cell culture	1191:1226	The results of this study highlight the feasibility of using a glycoengineered plant cell culture to produce recombinant proteins for which M3 or mannose receptor-mediated delivery is desired.					
34335667	5	18	theme	mM	930:931	arg1	concentration					910:922	a final concentration	902:922	a final concentration of 50 mM	902:931	Supplementing the culture medium with NaCl to a final concentration of 50 mM successfully increased GAA production by 3.8-fold.					
34335667	6	19	theme	NaCl	1077:1080	arg1	supplementation					1082:1096	the NaCl supplementation	1073:1096	the NaCl supplementation	1073:1096	GAA from an NaCl-supplemented culture showed a similar N-glycan profile, indicating that the NaCl supplementation did not affect N-glycosylation.					
34335667	2	20	theme	cellular	475:482	arg1	delivery					484:491	cellular delivery	475:491	cellular delivery of biopharmaceutics	475:511	However, the use of this platform is hindered by the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity.					
34335667	7	21	theme	recombinant	1239:1249	arg1	proteins					1251:1258	recombinant proteins	1239:1258	recombinant proteins for which M3 or mannose receptor-mediated delivery is desired	1239:1320	The results of this study highlight the feasibility of using a glycoengineered plant cell culture to produce recombinant proteins for which M3 or mannose receptor-mediated delivery is desired.					
34335667	3	22	theme	alternative	554:564	arg1	GAA					590:592	GAA	590:592	GAA	590:592	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	3	22	theme	alternative	554:564	arg1	glucosidase					577:587	an alternative acid-alpha glucosidase	551:587	an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease	551:641	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	5	23	theme	culture	874:880	arg1	medium					882:887	the culture medium	870:887	the culture medium with NaCl	870:897	Supplementing the culture medium with NaCl to a final concentration of 50 mM successfully increased GAA production by 3.8-fold.					
34335667	2	24	theme	inability	430:438	arg1	generation					386:395	the generation	382:395	the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity	382:533	However, the use of this platform is hindered by the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity.					
34335667	3	25	theme	acid-alpha	566:575	arg1	GAA					590:592	GAA	590:592	GAA	590:592	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	3	25	theme	acid-alpha	566:575	arg1	glucosidase					577:587	an alternative acid-alpha glucosidase	551:587	an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease	551:641	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	0	26	theme	Acid-Alpha	20:29	arg1	Glucosidase					31:41	Human Acid-Alpha Glucosidase	14:41	Human Acid-Alpha Glucosidase With a Paucimannose Structure	14:71	Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture.					
34335667	5	27	theme	GAA	956:958	arg1	production					960:969	GAA production	956:969	GAA production by 3.8-fold	956:981	Supplementing the culture medium with NaCl to a final concentration of 50 mM successfully increased GAA production by 3.8-fold.					
34335667	2	28	theme	N-glycans	415:423	arg1	generation					386:395	the generation	382:395	the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity	382:533	However, the use of this platform is hindered by the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity.					
34335667	0	29	theme	Human	14:18	arg1	Glucosidase					31:41	Human Acid-Alpha Glucosidase	14:41	Human Acid-Alpha Glucosidase With a Paucimannose Structure	14:71	Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture.					
34335667	3	30	theme	disease	635:641	arg1	therapy					618:624	enzyme replacement therapy	599:624	enzyme replacement therapy of Pompe disease	599:641	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	1	31	theme	Plant	118:122	arg1	cultures					129:136	Plant cell cultures	118:136	Plant cell cultures	118:136	Plant cell cultures have emerged as a promising platform for the production of biopharmaceutics due to their cost-effectiveness, safety, ability to control the cultivation, and secrete products into culture medium.					
34335667	2	32	theme	plant-specific	400:413	arg1	N-glycans					415:423	plant-specific N-glycans	400:423	plant-specific N-glycans	400:423	However, the use of this platform is hindered by the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity.					
34335667	0	33	theme	Glucosidase	31:41	arg1	Production					0:9	Production	0:9	Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture	0:115	Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture.					
34335667	6	34	theme	N-glycan	1039:1046	arg1	profile					1048:1054	a similar N-glycan profile	1029:1054	a similar N-glycan profile	1029:1054	GAA from an NaCl-supplemented culture showed a similar N-glycan profile, indicating that the NaCl supplementation did not affect N-glycosylation.					
34335667	0	35	theme	Paucimannose	50:61	arg1	Structure					63:71	a Paucimannose Structure	48:71	a Paucimannose Structure	48:71	Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture.					
34335667	7	36	theme	cell	1215:1218	arg1	culture					1220:1226	a glycoengineered plant cell culture	1191:1226	a glycoengineered plant cell culture	1191:1226	The results of this study highlight the feasibility of using a glycoengineered plant cell culture to produce recombinant proteins for which M3 or mannose receptor-mediated delivery is desired.					
34335667	3	37	theme	Pompe	629:633	arg1	disease					635:641	Pompe disease	629:641	Pompe disease	629:641	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	1	38	theme	cell	124:127	arg1	cultures					129:136	Plant cell cultures	118:136	Plant cell cultures	118:136	Plant cell cultures have emerged as a promising platform for the production of biopharmaceutics due to their cost-effectiveness, safety, ability to control the cultivation, and secrete products into culture medium.					
34335667	7	39	theme	study	1150:1154	arg1	results					1134:1140	The results	1130:1140	The results of this study	1130:1154	The results of this study highlight the feasibility of using a glycoengineered plant cell culture to produce recombinant proteins for which M3 or mannose receptor-mediated delivery is desired.					
34335667	1	40	theme	culture	317:323	arg1	medium					325:330	culture medium	317:330	culture medium	317:330	Plant cell cultures have emerged as a promising platform for the production of biopharmaceutics due to their cost-effectiveness, safety, ability to control the cultivation, and secrete products into culture medium.					
34335667	5	41	with	medium	882:887	arg1	NaCl					894:897	NaCl	894:897	NaCl	894:897	Supplementing the culture medium with NaCl to a final concentration of 50 mM successfully increased GAA production by 3.8-fold.					
34335667	3	42	theme	glycoengineered	661:675	arg1	culture					699:705	a glycoengineered Arabidopsis alg3 cell culture	659:705	a glycoengineered Arabidopsis alg3 cell culture	659:705	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	3	43	theme	replacement	606:616	arg1	therapy					618:624	enzyme replacement therapy	599:624	enzyme replacement therapy of Pompe disease	599:641	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	7	44	theme	mannose	1276:1282	arg1	delivery					1302:1309	M3 or mannose receptor-mediated delivery	1270:1309	M3 or mannose receptor-mediated delivery	1270:1309	The results of this study highlight the feasibility of using a glycoengineered plant cell culture to produce recombinant proteins for which M3 or mannose receptor-mediated delivery is desired.					
34335667	6	45	theme	similar	1031:1037	arg1	profile					1048:1054	a similar N-glycan profile	1029:1054	a similar N-glycan profile	1029:1054	GAA from an NaCl-supplemented culture showed a similar N-glycan profile, indicating that the NaCl supplementation did not affect N-glycosylation.					
34335667	4	46	theme	N-glycan	712:719	arg1	composition					721:731	The N-glycan composition	708:731	The N-glycan composition of the GAA	708:742	The N-glycan composition of the GAA consisted of a predominantly paucimannosidic structure, Man3GlcNAc2 (M3), without the plant-specific N-glycans.					
34335667	2	47	theme	biopharmaceutics	496:511	arg1	delivery					484:491	cellular delivery	475:491	cellular delivery of biopharmaceutics	475:511	However, the use of this platform is hindered by the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity.					
34335667	1	48	theme	biopharmaceutics	197:212	arg1	production					183:192	the production	179:192	the production of biopharmaceutics due to their cost-effectiveness, safety, ability to control the cultivation, and secrete products into culture medium	179:330	Plant cell cultures have emerged as a promising platform for the production of biopharmaceutics due to their cost-effectiveness, safety, ability to control the cultivation, and secrete products into culture medium.					
34335667	2	49	theme	platform	358:365	arg1	use					346:348	the use	342:348	the use of this platform	342:365	However, the use of this platform is hindered by the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity.					
34335667	2	50	theme	essential	451:459	arg1	N-glycans					461:469	essential N-glycans	451:469	essential N-glycans for cellular delivery of biopharmaceutics	451:511	However, the use of this platform is hindered by the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity.					
34335667	3	51	theme	cell	694:697	arg1	culture					699:705	a glycoengineered Arabidopsis alg3 cell culture	659:705	a glycoengineered Arabidopsis alg3 cell culture	659:705	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	3	52	theme	enzyme	599:604	arg1	therapy					618:624	enzyme replacement therapy	599:624	enzyme replacement therapy of Pompe disease	599:641	In this study, an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease was produced in a glycoengineered Arabidopsis alg3 cell culture.					
34335667	2	53	theme	low	518:520	arg1	productivity					522:533	low productivity	518:533	low productivity	518:533	However, the use of this platform is hindered by the generation of plant-specific N-glycans, the inability to produce essential N-glycans for cellular delivery of biopharmaceutics, and low productivity.					
33689337	0	0	from	Dynamics	11:18	arg1	Membrane					134:141	a Viral Membrane	126:141	a Viral Membrane	126:141	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	4	1	theme	flexible	823:830	arg1	stalk					832:836	the highly flexible stalk	812:836	the highly flexible stalk	812:836	Our simulations reveal that the highly flexible stalk is composed of two independent joints and most probable S protein orientations are competent for ACE2 binding.					
33689337	5	2	theme	closed	1006:1011	arg1	states					1013:1018	the open and/or closed states	990:1018	the open and/or closed states of the RBD	990:1029	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	2	3	theme	protein	441:447	arg1	models					449:454	fully glycosylated full-length SARS-CoV-2 S protein models	397:454	fully glycosylated full-length SARS-CoV-2 S protein models	397:454	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	3	4	theme	μs-long	627:633	arg1	simulations					663:673	multiple μs-long all-atom molecular dynamics simulations	618:673	multiple μs-long all-atom molecular dynamics simulations	618:673	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	2	5	theme	viral	461:465	arg1	membrane					467:474	a viral membrane	459:474	a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region	459:601	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	0	6	theme	Glycosylated	73:84	arg1	Protein					115:121	the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein	63:121	the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein	63:121	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	0	7	from	Structure	0:8	arg1	Membrane					134:141	a Viral Membrane	126:141	a Viral Membrane	126:141	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	2	8	gly	glycosylated	403:414	arg1	models					449:454	fully glycosylated full-length SARS-CoV-2 S protein models	397:454	fully glycosylated full-length SARS-CoV-2 S protein models	397:454	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	3	9	theme	glycan	766:771	arg1	functions					773:781	glycan functions	766:781	glycan functions	766:781	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	4	10	theme	S	894:894	arg1	orientations					904:915	most probable S protein orientations	880:915	most probable S protein orientations	880:915	Our simulations reveal that the highly flexible stalk is composed of two independent joints and most probable S protein orientations are competent for ACE2 binding.					
33689337	5	11	theme	surface	1181:1187	arg1	area					1189:1192	commonly used accessible surface area	1156:1192	detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies	1105:1332	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	0	12	theme	SARS-CoV-2	98:107	arg1	Protein					115:121	the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein	63:121	the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein	63:121	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	3	13	dep	structure	726:734	arg1	the					722:724	the	722:724	the	722:724	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	2	14	theme	closed	500:505	arg1	conformations					507:519	open and closed conformations	491:519	open and closed conformations of the receptor-binding domain (RBD)	491:556	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	2	15	theme	stalk	590:594	arg1	region					596:601	the stalk region	586:601	the stalk region	586:601	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	1	16	theme	spike	148:152	arg1	protein					158:164	The spike (S) protein	144:164	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	144:228	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	0	17	theme	Full-Length	86:96	arg1	Protein					115:121	the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein	63:121	the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein	63:121	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	0	18	from	Binding	30:36	arg1	Membrane					134:141	a Viral Membrane	126:141	a Viral Membrane	126:141	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	5	19	theme	antibody	1068:1075	arg1	epitopes					1077:1084	antibody epitopes	1068:1084	antibody epitopes	1068:1084	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	5	20	theme	detailed	1105:1112	arg1	analysis					1136:1143	detailed antibody-glycan clash analysis	1105:1143	detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies	1105:1332	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	4	21	theme	ACE2	935:938	arg1	binding					940:946	ACE2 binding	935:946	ACE2 binding	935:946	Our simulations reveal that the highly flexible stalk is composed of two independent joints and most probable S protein orientations are competent for ACE2 binding.					
33689337	5	22	theme	clash	1130:1134	arg1	analysis					1136:1143	detailed antibody-glycan clash analysis	1105:1143	detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies	1105:1332	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	2	23	theme	receptor-binding	528:543	arg1	RBD					553:555	RBD	553:555	RBD	553:555	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	2	23	theme	receptor-binding	528:543	arg1	domain					545:550	the receptor-binding domain	524:550	the receptor-binding domain (RBD)	524:556	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	3	24	theme	all-atom	635:642	arg1	dynamics					654:661	all-atom molecular dynamics	635:661	multiple μs-long all-atom molecular dynamics simulations	618:673	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	1	25	theme	host	239:242	arg1	entry					249:253	host cell entry	239:253	host cell entry	239:253	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	2	26	theme	different	562:570	arg1	templates					572:580	different templates	562:580	different templates for the stalk region	562:601	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	5	27	theme	RBD	1027:1029	arg1	states					1013:1018	the open and/or closed states	990:1018	the open and/or closed states of the RBD	990:1029	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	6	28	theme	S	1419:1419	arg1	protein					1421:1427	the SARS-CoV-2 S protein	1404:1427	the SARS-CoV-2 S protein	1404:1427	Overall, our observations offer structural and dynamic insights into the SARS-CoV-2 S protein and potentialize for guiding the design of effective antiviral therapeutics.					
33689337	1	29	theme	cell	244:247	arg1	entry					249:253	host cell entry	239:253	host cell entry	239:253	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	0	30	theme	Protein	115:121	arg1	Dynamics					11:18	Dynamics	11:18	Dynamics	11:18	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	0	30	theme	Protein	115:121	arg1	Binding					30:36	Receptor Binding	21:36	Receptor Binding	21:36	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	0	30	theme	Protein	115:121	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	0	30	theme	Protein	115:121	arg1	Binding					52:58	Antibody Binding	43:58	Antibody Binding	43:58	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	5	31	theme	open	994:997	arg1	states					1013:1018	the open and/or closed states	990:1018	the open and/or closed states of the RBD	990:1029	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	5	32	theme	possible	1273:1280	arg1	interactions					1291:1302	possible detailed interactions	1273:1302	possible detailed interactions between glycan and antibodies	1273:1332	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	6	33	theme	SARS-CoV-2	1408:1417	arg1	protein					1421:1427	the SARS-CoV-2 S protein	1404:1427	the SARS-CoV-2 S protein	1404:1427	Overall, our observations offer structural and dynamic insights into the SARS-CoV-2 S protein and potentialize for guiding the design of effective antiviral therapeutics.					
33689337	5	34	theme	epitopes	1077:1084	arg1	exposure					1056:1063	the exposure	1052:1063	the exposure of antibody epitopes	1052:1084	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	0	35	theme	Spike	109:113	arg1	Protein					115:121	the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein	63:121	the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein	63:121	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	1	36	theme	major	330:334	arg1	target					336:341	the major target	326:341	the major target for drug and vaccine development	326:374	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	5	37	theme	detailed	1282:1289	arg1	interactions					1291:1302	possible detailed interactions	1273:1302	possible detailed interactions between glycan and antibodies	1273:1332	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	2	38	theme	S	439:439	arg1	protein					441:447	full-length SARS-CoV-2 S protein	416:447	fully glycosylated full-length SARS-CoV-2 S protein models	397:454	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	3	39	theme	deeper	701:706	arg1	insights					708:715	deeper insights	701:715	deeper insights into the structure and dynamics of S protein and glycan functions	701:781	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	4	40	theme	probable	885:892	arg1	orientations					904:915	most probable S protein orientations	880:915	most probable S protein orientations	880:915	Our simulations reveal that the highly flexible stalk is composed of two independent joints and most probable S protein orientations are competent for ACE2 binding.					
33689337	5	41	theme	area	1189:1192	arg1	analysis					1194:1201	commonly used accessible surface area analysis	1156:1201	detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies	1105:1332	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	4	42	theme	most	880:883	arg1	orientations					904:915	most probable S protein orientations	880:915	most probable S protein orientations	880:915	Our simulations reveal that the highly flexible stalk is composed of two independent joints and most probable S protein orientations are competent for ACE2 binding.					
33689337	6	43	theme	therapeutics	1492:1503	arg1	design					1462:1467	the design	1458:1467	the design of effective antiviral therapeutics	1458:1503	Overall, our observations offer structural and dynamic insights into the SARS-CoV-2 S protein and potentialize for guiding the design of effective antiviral therapeutics.					
33689337	1	44	theme	severe	169:174	arg1	coronavirus					203:213	severe acute respiratory syndrome coronavirus 2	169:215	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	169:228	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	1	44	theme	severe	169:174	arg1	SARS-CoV-2					218:227	SARS-CoV-2	218:227	SARS-CoV-2	218:227	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	0	45	theme	Receptor	21:28	arg1	Binding					30:36	Receptor Binding	21:36	Receptor Binding	21:36	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	6	46	theme	structural	1367:1376	arg1	insights					1390:1397	structural and dynamic insights	1367:1397	structural and dynamic insights into the SARS-CoV-2 S protein	1367:1427	Overall, our observations offer structural and dynamic insights into the SARS-CoV-2 S protein and potentialize for guiding the design of effective antiviral therapeutics.					
33689337	2	47	theme	SARS-CoV-2	428:437	arg1	protein					441:447	full-length SARS-CoV-2 S protein	416:447	fully glycosylated full-length SARS-CoV-2 S protein models	397:454	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	0	48	from	Binding	52:58	arg1	Membrane					134:141	a Viral Membrane	126:141	a Viral Membrane	126:141	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	1	49	theme	acute	176:180	arg1	coronavirus					203:213	severe acute respiratory syndrome coronavirus 2	169:215	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	169:228	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	1	49	theme	acute	176:180	arg1	SARS-CoV-2					218:227	SARS-CoV-2	218:227	SARS-CoV-2	218:227	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	2	50	theme	full-length	416:426	arg1	protein					441:447	full-length SARS-CoV-2 S protein	416:447	fully glycosylated full-length SARS-CoV-2 S protein models	397:454	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	4	51	theme	protein	896:902	arg1	orientations					904:915	most probable S protein orientations	880:915	most probable S protein orientations	880:915	Our simulations reveal that the highly flexible stalk is composed of two independent joints and most probable S protein orientations are competent for ACE2 binding.					
33689337	2	52	theme	domain	545:550	arg1	templates					572:580	different templates	562:580	different templates for the stalk region	562:601	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	2	52	theme	domain	545:550	arg1	conformations					507:519	open and closed conformations	491:519	open and closed conformations of the receptor-binding domain (RBD)	491:556	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	1	53	theme	drug	347:350	arg1	development					364:374	drug and vaccine development	347:374	development	364:374	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	1	54	dep	spike	148:152	arg1	S					155:155	S	155:155	S	155:155	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	5	55	theme	used	1165:1168	arg1	area					1189:1192	commonly used accessible surface area	1156:1192	detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies	1105:1332	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	2	56	theme	open	491:494	arg1	conformations					507:519	open and closed conformations	491:519	open and closed conformations of the receptor-binding domain (RBD)	491:556	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	1	57	theme	angiotensin-converting	269:290	arg1	ACE2					302:305	ACE2	302:305	ACE2	302:305	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	1	57	theme	angiotensin-converting	269:290	arg1	enzyme					292:297	angiotensin-converting enzyme 2	269:299	angiotensin-converting enzyme 2 (ACE2)	269:306	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	0	58	theme	Viral	128:132	arg1	Membrane					134:141	a Viral Membrane	126:141	a Viral Membrane	126:141	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	2	59	theme	glycosylated	403:414	arg1	models					449:454	fully glycosylated full-length SARS-CoV-2 S protein models	397:454	fully glycosylated full-length SARS-CoV-2 S protein models	397:454	We previously built fully glycosylated full-length SARS-CoV-2 S protein models in a viral membrane including both open and closed conformations of the receptor-binding domain (RBD) and different templates for the stalk region.					
33689337	3	60	theme	multiple	618:625	arg1	simulations					663:673	multiple μs-long all-atom molecular dynamics simulations	618:673	multiple μs-long all-atom molecular dynamics simulations	618:673	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	6	61	theme	antiviral	1482:1490	arg1	therapeutics					1492:1503	effective antiviral therapeutics	1472:1503	effective antiviral therapeutics	1472:1503	Overall, our observations offer structural and dynamic insights into the SARS-CoV-2 S protein and potentialize for guiding the design of effective antiviral therapeutics.					
33689337	5	62	theme	antibody-glycan	1114:1128	arg1	analysis					1136:1143	detailed antibody-glycan clash analysis	1105:1143	detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies	1105:1332	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	4	63	theme	independent	857:867	arg1	joints					869:874	two independent joints	853:874	two independent joints	853:874	Our simulations reveal that the highly flexible stalk is composed of two independent joints and most probable S protein orientations are competent for ACE2 binding.					
33689337	1	64	theme	vaccine	356:362	arg1	development					364:374	drug and vaccine development	347:374	development	364:374	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	5	65	theme	accessible	1170:1179	arg1	area					1189:1192	commonly used accessible surface area	1156:1192	detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies	1105:1332	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	5	66	theme	glycan	1244:1249	arg1	shielding					1251:1259	glycan shielding	1244:1259	glycan shielding	1244:1259	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	1	67	theme	respiratory	182:192	arg1	coronavirus					203:213	severe acute respiratory syndrome coronavirus 2	169:215	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	169:228	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	1	67	theme	respiratory	182:192	arg1	SARS-CoV-2					218:227	SARS-CoV-2	218:227	SARS-CoV-2	218:227	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	5	68	theme	shielding	1251:1259	arg1	impact					1234:1239	the impact	1230:1239	the impact of glycan shielding	1230:1259	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	1	69	theme	syndrome	194:201	arg1	coronavirus					203:213	severe acute respiratory syndrome coronavirus 2	169:215	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	169:228	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	1	69	theme	syndrome	194:201	arg1	SARS-CoV-2					218:227	SARS-CoV-2	218:227	SARS-CoV-2	218:227	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	6	70	theme	dynamic	1382:1388	arg1	insights					1390:1397	structural and dynamic insights	1367:1397	structural and dynamic insights into the SARS-CoV-2 S protein	1367:1427	Overall, our observations offer structural and dynamic insights into the SARS-CoV-2 S protein and potentialize for guiding the design of effective antiviral therapeutics.					
33689337	6	71	theme	effective	1472:1480	arg1	therapeutics					1492:1503	effective antiviral therapeutics	1472:1503	effective antiviral therapeutics	1472:1503	Overall, our observations offer structural and dynamic insights into the SARS-CoV-2 S protein and potentialize for guiding the design of effective antiviral therapeutics.					
33689337	1	72	theme	coronavirus	203:213	arg1	protein					158:164	The spike (S) protein	144:164	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	144:228	The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates host cell entry by binding to angiotensin-converting enzyme 2 (ACE2) and is considered the major target for drug and vaccine development.					
33689337	0	73	theme	Antibody	43:50	arg1	Binding					52:58	Antibody Binding	43:58	Antibody Binding	43:58	Structure, Dynamics, Receptor Binding, and Antibody Binding of the Fully Glycosylated Full-Length SARS-CoV-2 Spike Protein in a Viral Membrane.					
33689337	3	74	theme	molecular	644:652	arg1	dynamics					654:661	all-atom molecular dynamics	635:661	multiple μs-long all-atom molecular dynamics simulations	618:673	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	5	75	theme	multiple	961:968	arg1	glycans					970:976	multiple glycans	961:976	multiple glycans stabilizing the open and/or closed states of the RBD	961:1029	We identify multiple glycans stabilizing the open and/or closed states of the RBD and demonstrate that the exposure of antibody epitopes can be captured by detailed antibody-glycan clash analysis instead of commonly used accessible surface area analysis that tends to overestimate the impact of glycan shielding and neglect possible detailed interactions between glycan and antibodies.					
33689337	3	76	theme	dynamics	654:661	arg1	simulations					663:673	multiple μs-long all-atom molecular dynamics simulations	618:673	multiple μs-long all-atom molecular dynamics simulations	618:673	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	3	77	theme	protein	754:760	arg1	structure					726:734	structure	726:734	structure	726:734	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	3	77	theme	protein	754:760	arg1	dynamics					740:747	dynamics	740:747	dynamics	740:747	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	3	77	theme	protein	754:760	arg1	functions					773:781	glycan functions	766:781	glycan functions	766:781	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
33689337	3	78	theme	S	752:752	arg1	protein					754:760	S protein	752:760	S protein	752:760	In this work, multiple μs-long all-atom molecular dynamics simulations were performed to provide deeper insights into the structure and dynamics of S protein and glycan functions.					
34000456	6	0	theme	suppressed	955:964	arg1	levels					966:971	suppressed levels	955:971	suppressed levels of multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray	955:1056	Glycomic analysis consistently revealed suppressed levels of multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray.					
34000456	4	1	theme	glycoproteins	601:613	arg1	dysregulation					550:562	the dysregulation	546:562	the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells	546:655	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	7	2	theme	PTX	1224:1226	arg1	cells					1243:1247	PTX resistant MCF7 cells	1224:1247	PTX resistant MCF7 cells	1224:1247	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	12	3	theme	valuable	1959:1966	arg1	information					1968:1978	valuable information	1959:1978	valuable information to understand molecular mechanisms underlying chemoresistance in BC	1959:2046	This study might provide valuable information to understand molecular mechanisms underlying chemoresistance in BC.					
34000456	11	4	theme	glycan	1863:1868	arg1	signatures					1887:1896	unique glycan-related protein, glycan and glycoprotein signatures	1832:1896	signatures	1887:1896	In this multi-omic analysis, we identified unique glycan-related protein, glycan and glycoprotein signatures defining PTX chemoresistance in BC.					
34000456	5	5	theme	MCF7	688:691	arg1	cells					693:697	paclitaxel (PTX) resistant MCF7 cells	661:697	paclitaxel (PTX) resistant MCF7 cells	661:697	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	6	6	theme	Glycomic	915:922	arg1	analysis					924:931	Glycomic analysis	915:931	Glycomic analysis	915:931	Glycomic analysis consistently revealed suppressed levels of multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray.					
34000456	8	7	theme	glycoproteins	1353:1365	arg1	levels					1289:1294	the aberrant levels	1276:1294	the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance	1276:1383	Our findings demonstrated the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance.					
34000456	11	8	theme	protein	1854:1860	arg1	signatures					1887:1896	unique glycan-related protein, glycan and glycoprotein signatures	1832:1896	signatures	1887:1896	In this multi-omic analysis, we identified unique glycan-related protein, glycan and glycoprotein signatures defining PTX chemoresistance in BC.					
34000456	2	9	theme	glycans	266:272	arg1	levels					256:261	Aberrant levels	247:261	Aberrant levels of glycans	247:272	Aberrant levels of glycans are closely correlated with chemoresistance.					
34000456	5	10	theme	PTX	893:895	arg1	cells					908:912	PTX treated BC cells	893:912	PTX treated BC cells	893:912	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	8	11	theme	PTX	1370:1372	arg1	resistance					1374:1383	PTX resistance	1370:1383	PTX resistance	1370:1383	Our findings demonstrated the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance.					
34000456	7	12	theme	signaling	1179:1187	arg1	pathway					1189:1195	ERK signaling pathway	1175:1195	ERK signaling pathway	1175:1195	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	5	13	theme	BC	905:906	arg1	cells					908:912	PTX treated BC cells	893:912	PTX treated BC cells	893:912	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	8	14	theme	multi-antennary	1299:1313	arg1	structures					1325:1334	multi-antennary branching structures	1299:1334	multi-antennary branching structures	1299:1334	Our findings demonstrated the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance.					
34000456	5	15	theme	N-glycan-related	728:743	arg1	proteins					745:752	19 differentially expressed N-glycan-related proteins	700:752	19 differentially expressed N-glycan-related proteins	700:752	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	8	16	theme	structures	1325:1334	arg1	levels					1289:1294	the aberrant levels	1276:1294	the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance	1276:1383	Our findings demonstrated the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance.					
34000456	7	17	theme	suppressed	1096:1105	arg1	levels					1107:1112	suppressed levels	1096:1112	suppressed levels of multi-antennary branching structures	1096:1152	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	1	18	theme	high	210:213	arg1	rates					215:219	the high rates	206:219	the high rates of cancer-related deaths	206:244	Chemoresistance is a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths.					
34000456	6	19	theme	branching	992:1000	arg1	structures					1002:1011	multi-antennary branching structures	976:1011	multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray	976:1056	Glycomic analysis consistently revealed suppressed levels of multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray.					
34000456	4	20	theme	transcriptomics	464:478	arg1	combination					449:459	a combination	447:459	a combination of transcriptomics, proteomics, glycomics and glycoproteomics	447:521	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	7	21	theme	structures	1143:1152	arg1	levels					1107:1112	suppressed levels	1096:1112	suppressed levels of multi-antennary branching structures	1096:1152	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	5	22	from	expression	865:874	arg1	cells					908:912	PTX treated BC cells	893:912	PTX treated BC cells	893:912	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	4	23	theme	integrated	422:431	arg1	strategy					433:440	an integrated strategy	419:440	an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics	419:521	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	7	24	theme	multi-antennary	1117:1131	arg1	structures					1143:1152	multi-antennary branching structures	1117:1152	multi-antennary branching structures	1117:1152	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	7	25	theme	Several	1059:1065	arg1	glycoproteins					1074:1086	Several target glycoproteins	1059:1086	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures	1059:1152	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	11	26	from	chemoresistance	1911:1925	arg1	BC					1930:1931	BC	1930:1931	BC	1930:1931	In this multi-omic analysis, we identified unique glycan-related protein, glycan and glycoprotein signatures defining PTX chemoresistance in BC.					
34000456	9	27	theme	multi-omic	1413:1422	arg1	analysis					1424:1431	Systematically integrative multi-omic analysis	1386:1431	Systematically integrative multi-omic analysis	1386:1431	Systematically integrative multi-omic analysis is expected to facilitate the discovery of the aberrant glycosyltransferases, N-glycosylation and glycoproteins in tumor progression and chemoresistance.					
34000456	3	28	theme	essential	323:331	arg1	functions					333:341	The essential functions	319:341	The essential functions of glycans in chemoresistance	319:371	The essential functions of glycans in chemoresistance is not systematically studied.					
34000456	4	29	theme	glycoproteomics	507:521	arg1	combination					449:459	a combination	447:459	a combination of transcriptomics, proteomics, glycomics and glycoproteomics	447:521	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	0	30	theme	paclitaxel	87:96	arg1	resistance					98:107	paclitaxel resistance	87:107	paclitaxel resistance in breast cancer cells	87:130	Integrating transcriptomics, proteomics, glycomics and glycoproteomics to characterize paclitaxel resistance in breast cancer cells.					
34000456	1	31	theme	breast	175:180	arg1	BC					190:191	BC	190:191	BC	190:191	Chemoresistance is a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths.					
34000456	1	31	theme	breast	175:180	arg1	cancer					182:187	breast cancer	175:187	breast cancer (BC) relapse	175:200	Chemoresistance is a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths.					
34000456	10	32	theme	integrated	1604:1613	arg1	strategy					1615:1622	An integrated strategy	1601:1622	An integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics	1601:1703	SIGNIFICANCE: An integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics is crucial to understand the association between glycans and chemoresistance in BC.					
34000456	10	32	theme	integrated	1604:1613	arg1	crucial					1708:1714	crucial	1708:1714	crucial	1708:1714	SIGNIFICANCE: An integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics is crucial to understand the association between glycans and chemoresistance in BC.					
34000456	4	33	theme	proteomics	481:490	arg1	combination					449:459	a combination	447:459	a combination of transcriptomics, proteomics, glycomics and glycoproteomics	447:521	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	10	34	theme	transcriptomics	1646:1660	arg1	combination					1631:1641	a combination	1629:1641	a combination of transcriptomics, proteomics, glycomics and glycoproteomics	1629:1703	SIGNIFICANCE: An integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics is crucial to understand the association between glycans and chemoresistance in BC.					
34000456	0	35	theme	cancer	119:124	arg1	cells					126:130	breast cancer cells	112:130	breast cancer cells	112:130	Integrating transcriptomics, proteomics, glycomics and glycoproteomics to characterize paclitaxel resistance in breast cancer cells.					
34000456	4	36	theme	glycomics	493:501	arg1	combination					449:459	a combination	447:459	a combination of transcriptomics, proteomics, glycomics and glycoproteomics	447:521	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	0	37	theme	Integrating	0:10	arg1	transcriptomics					12:26	Integrating transcriptomics	0:26	Integrating transcriptomics	0:26	Integrating transcriptomics, proteomics, glycomics and glycoproteomics to characterize paclitaxel resistance in breast cancer cells.					
34000456	4	38	theme	glycogenes	567:576	arg1	dysregulation					550:562	the dysregulation	546:562	the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells	546:655	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	10	39	theme	glycomics	1675:1683	arg1	combination					1631:1641	a combination	1629:1641	a combination of transcriptomics, proteomics, glycomics and glycoproteomics	1629:1703	SIGNIFICANCE: An integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics is crucial to understand the association between glycans and chemoresistance in BC.					
34000456	9	40	gly	glycoproteins	1531:1543	arg1	glycosyltransferases					1489:1508	the aberrant glycosyltransferases	1476:1508	the aberrant glycosyltransferases	1476:1508	Systematically integrative multi-omic analysis is expected to facilitate the discovery of the aberrant glycosyltransferases, N-glycosylation and glycoproteins in tumor progression and chemoresistance.					
34000456	9	40	gly	glycoproteins	1531:1543	arg1	glycoproteins					1531:1543	glycoproteins	1531:1543	glycoproteins	1531:1543	Systematically integrative multi-omic analysis is expected to facilitate the discovery of the aberrant glycosyltransferases, N-glycosylation and glycoproteins in tumor progression and chemoresistance.					
34000456	5	41	theme	MGAT4A	858:863	arg1	expression					865:874	MGAT4A expression	858:874	MGAT4A expression at mRNA level in PTX treated BC cells	858:912	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	9	42	theme	aberrant	1480:1487	arg1	N-glycosylation					1511:1525	N-glycosylation	1511:1525	N-glycosylation	1511:1525	Systematically integrative multi-omic analysis is expected to facilitate the discovery of the aberrant glycosyltransferases, N-glycosylation and glycoproteins in tumor progression and chemoresistance.					
34000456	9	42	theme	aberrant	1480:1487	arg1	glycosyltransferases					1489:1508	the aberrant glycosyltransferases	1476:1508	the aberrant glycosyltransferases	1476:1508	Systematically integrative multi-omic analysis is expected to facilitate the discovery of the aberrant glycosyltransferases, N-glycosylation and glycoproteins in tumor progression and chemoresistance.					
34000456	9	42	theme	aberrant	1480:1487	arg1	glycoproteins					1531:1543	glycoproteins	1531:1543	glycoproteins	1531:1543	Systematically integrative multi-omic analysis is expected to facilitate the discovery of the aberrant glycosyltransferases, N-glycosylation and glycoproteins in tumor progression and chemoresistance.					
34000456	4	43	theme	glycan	579:584	arg1	structures					586:595	glycan structures	579:595	glycan structures	579:595	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	10	44	theme	proteomics	1663:1672	arg1	combination					1631:1641	a combination	1629:1641	a combination of transcriptomics, proteomics, glycomics and glycoproteomics	1629:1703	SIGNIFICANCE: An integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics is crucial to understand the association between glycans and chemoresistance in BC.					
34000456	11	45	theme	PTX	1907:1909	arg1	chemoresistance					1911:1925	PTX chemoresistance	1907:1925	PTX chemoresistance in BC	1907:1931	In this multi-omic analysis, we identified unique glycan-related protein, glycan and glycoprotein signatures defining PTX chemoresistance in BC.					
34000456	4	46	with	strategy	433:440	arg1	combination					449:459	a combination	447:459	a combination of transcriptomics, proteomics, glycomics and glycoproteomics	447:521	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	4	47	from	dysregulation	550:562	arg1	chemoresistance					618:632	chemoresistance	618:632	chemoresistance of breast cancer cells	618:655	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	10	48	from	association	1734:1744	arg1	BC					1785:1786	BC	1785:1786	BC	1785:1786	SIGNIFICANCE: An integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics is crucial to understand the association between glycans and chemoresistance in BC.					
34000456	7	49	theme	MCF7	1238:1241	arg1	cells					1243:1247	PTX resistant MCF7 cells	1224:1247	PTX resistant MCF7 cells	1224:1247	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	7	50	gly	glycoproteins	1074:1086	arg1	glycoproteins					1074:1086	Several target glycoproteins	1059:1086	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures	1059:1152	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	10	51	theme	glycoproteomics	1689:1703	arg1	combination					1631:1641	a combination	1629:1641	a combination of transcriptomics, proteomics, glycomics and glycoproteomics	1629:1703	SIGNIFICANCE: An integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics is crucial to understand the association between glycans and chemoresistance in BC.					
34000456	1	52	theme	cancer-related	224:237	arg1	deaths					239:244	cancer-related deaths	224:244	cancer-related deaths	224:244	Chemoresistance is a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths.					
34000456	4	53	theme	cancer	644:649	arg1	cells					651:655	breast cancer cells	637:655	breast cancer cells	637:655	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	11	54	theme	glycan-related	1839:1852	arg1	signatures					1887:1896	unique glycan-related protein, glycan and glycoprotein signatures	1832:1896	signatures	1887:1896	In this multi-omic analysis, we identified unique glycan-related protein, glycan and glycoprotein signatures defining PTX chemoresistance in BC.					
34000456	11	55	theme	multi-omic	1797:1806	arg1	analysis					1808:1815	this multi-omic analysis	1792:1815	this multi-omic analysis	1792:1815	In this multi-omic analysis, we identified unique glycan-related protein, glycan and glycoprotein signatures defining PTX chemoresistance in BC.					
34000456	8	56	from	levels	1289:1294	arg1	resistance					1374:1383	PTX resistance	1370:1383	PTX resistance	1370:1383	Our findings demonstrated the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance.					
34000456	7	57	theme	ERK	1175:1177	arg1	pathway					1189:1195	ERK signaling pathway	1175:1195	ERK signaling pathway	1175:1195	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	5	58	theme	resistant	678:686	arg1	cells					693:697	paclitaxel (PTX) resistant MCF7 cells	661:697	paclitaxel (PTX) resistant MCF7 cells	661:697	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	5	59	with	consistent	830:839	arg1	decrease					846:853	decrease	846:853	decrease in MGAT4A expression at mRNA level in PTX treated BC cells	846:912	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	1	60	theme	cancer	182:187	arg1	relapse					194:200	breast cancer (BC) relapse	175:200	breast cancer (BC) relapse	175:200	Chemoresistance is a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths.					
34000456	8	61	theme	target	1346:1351	arg1	glycoproteins					1353:1365	their target glycoproteins	1340:1365	their target glycoproteins	1340:1365	Our findings demonstrated the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance.					
34000456	5	62	theme	paclitaxel	661:670	arg1	cells					693:697	paclitaxel (PTX) resistant MCF7 cells	661:697	paclitaxel (PTX) resistant MCF7 cells	661:697	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	5	63	theme	treated	897:903	arg1	cells					908:912	PTX treated BC cells	893:912	PTX treated BC cells	893:912	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	8	64	theme	aberrant	1280:1287	arg1	levels					1289:1294	the aberrant levels	1276:1294	the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance	1276:1383	Our findings demonstrated the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance.					
34000456	6	65	theme	lectin	1040:1045	arg1	microarray					1047:1056	MALDI-TOF/TOF-MS and lectin microarray	1019:1056	MALDI-TOF/TOF-MS and lectin microarray	1019:1056	Glycomic analysis consistently revealed suppressed levels of multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray.					
34000456	5	66	theme	expressed	718:726	arg1	proteins					745:752	19 differentially expressed N-glycan-related proteins	700:752	19 differentially expressed N-glycan-related proteins	700:752	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	6	67	theme	MALDI-TOF/TOF-MS	1019:1034	arg1	microarray					1047:1056	MALDI-TOF/TOF-MS and lectin microarray	1019:1056	MALDI-TOF/TOF-MS and lectin microarray	1019:1056	Glycomic analysis consistently revealed suppressed levels of multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray.					
34000456	0	68	from	resistance	98:107	arg1	cells					126:130	breast cancer cells	112:130	breast cancer cells	112:130	Integrating transcriptomics, proteomics, glycomics and glycoproteomics to characterize paclitaxel resistance in breast cancer cells.					
34000456	8	69	gly	glycoproteins	1353:1365	arg1	glycoproteins					1353:1365	their target glycoproteins	1340:1365	their target glycoproteins	1340:1365	Our findings demonstrated the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance.					
34000456	5	70	theme	mRNA	879:882	arg1	level					884:888	mRNA level	879:888	mRNA level	879:888	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	8	71	theme	branching	1315:1323	arg1	structures					1325:1334	multi-antennary branching structures	1299:1334	multi-antennary branching structures	1299:1334	Our findings demonstrated the aberrant levels of multi-antennary branching structures and their target glycoproteins on PTX resistance.					
34000456	6	72	theme	structures	1002:1011	arg1	levels					966:971	suppressed levels	955:971	suppressed levels of multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray	955:1056	Glycomic analysis consistently revealed suppressed levels of multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray.					
34000456	7	73	theme	branching	1133:1141	arg1	structures					1143:1152	multi-antennary branching structures	1117:1152	multi-antennary branching structures	1117:1152	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	5	74	from	level	884:888	arg1	decrease					846:853	decrease	846:853	decrease in MGAT4A expression at mRNA level in PTX treated BC cells	846:912	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	5	74	from	level	884:888	arg1	expression					865:874	MGAT4A expression	858:874	MGAT4A expression at mRNA level in PTX treated BC cells	858:912	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	6	75	theme	multi-antennary	976:990	arg1	structures					1002:1011	multi-antennary branching structures	976:1011	multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray	976:1056	Glycomic analysis consistently revealed suppressed levels of multi-antennary branching structures using MALDI-TOF/TOF-MS and lectin microarray.					
34000456	3	76	from	functions	333:341	arg1	chemoresistance					357:371	chemoresistance	357:371	chemoresistance	357:371	The essential functions of glycans in chemoresistance is not systematically studied.					
34000456	9	77	theme	integrative	1401:1411	arg1	analysis					1424:1431	Systematically integrative multi-omic analysis	1386:1431	Systematically integrative multi-omic analysis	1386:1431	Systematically integrative multi-omic analysis is expected to facilitate the discovery of the aberrant glycosyltransferases, N-glycosylation and glycoproteins in tumor progression and chemoresistance.					
34000456	2	78	theme	Aberrant	247:254	arg1	levels					256:261	Aberrant levels	247:261	Aberrant levels of glycans	247:272	Aberrant levels of glycans are closely correlated with chemoresistance.					
34000456	7	79	theme	target	1067:1072	arg1	glycoproteins					1074:1086	Several target glycoproteins	1059:1086	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures	1059:1152	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	1	80	theme	major	154:158	arg1	factor					160:165	a major factor	152:165	a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths	152:244	Chemoresistance is a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths.					
34000456	1	80	theme	major	154:158	arg1	Chemoresistance					133:147	Chemoresistance	133:147	Chemoresistance	133:147	Chemoresistance is a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths.					
34000456	3	81	theme	glycans	346:352	arg1	functions					333:341	The essential functions	319:341	The essential functions of glycans in chemoresistance	319:371	The essential functions of glycans in chemoresistance is not systematically studied.					
34000456	11	82	gly	glycoprotein	1874:1885	arg1	glycoprotein					1874:1885	unique glycan-related protein, glycan and glycoprotein signatures	1832:1896	glycoprotein	1874:1885	In this multi-omic analysis, we identified unique glycan-related protein, glycan and glycoprotein signatures defining PTX chemoresistance in BC.					
34000456	0	83	theme	breast	112:117	arg1	cells					126:130	breast cancer cells	112:130	breast cancer cells	112:130	Integrating transcriptomics, proteomics, glycomics and glycoproteomics to characterize paclitaxel resistance in breast cancer cells.					
34000456	5	84	from	decrease	846:853	arg1	expression					865:874	MGAT4A expression	858:874	MGAT4A expression at mRNA level in PTX treated BC cells	858:912	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	5	84	from	decrease	846:853	arg1	level					884:888	mRNA level	879:888	mRNA level	879:888	In paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, of which MGAT4A was the most significantly down-regulated, consistent with decrease in MGAT4A expression at mRNA level in PTX treated BC cells.					
34000456	4	85	gly	glycoproteins	601:613	arg1	glycoproteins					601:613	glycoproteins	601:613	glycoproteins	601:613	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	10	86	with	strategy	1615:1622	arg1	combination					1631:1641	a combination	1629:1641	a combination of transcriptomics, proteomics, glycomics and glycoproteomics	1629:1703	SIGNIFICANCE: An integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics is crucial to understand the association between glycans and chemoresistance in BC.					
34000456	4	87	theme	structures	586:595	arg1	dysregulation					550:562	the dysregulation	546:562	the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells	546:655	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	9	88	theme	glycosyltransferases	1489:1508	arg1	discovery					1463:1471	the discovery	1459:1471	the discovery of the aberrant glycosyltransferases, N-glycosylation and glycoproteins	1459:1543	Systematically integrative multi-omic analysis is expected to facilitate the discovery of the aberrant glycosyltransferases, N-glycosylation and glycoproteins in tumor progression and chemoresistance.					
34000456	11	89	theme	glycoprotein	1874:1885	arg1	signatures					1887:1896	unique glycan-related protein, glycan and glycoprotein signatures	1832:1896	signatures	1887:1896	In this multi-omic analysis, we identified unique glycan-related protein, glycan and glycoprotein signatures defining PTX chemoresistance in BC.					
34000456	7	90	theme	resistant	1228:1236	arg1	cells					1243:1247	PTX resistant MCF7 cells	1224:1247	PTX resistant MCF7 cells	1224:1247	Several target glycoproteins bearing suppressed levels of multi-antennary branching structures were identified, and ERK signaling pathway was strongly suppressed in PTX resistant MCF7 cells.					
34000456	4	91	theme	breast	637:642	arg1	cells					651:655	breast cancer cells	637:655	breast cancer cells	637:655	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	9	92	theme	tumor	1548:1552	arg1	progression					1554:1564	tumor progression	1548:1564	tumor progression	1548:1564	Systematically integrative multi-omic analysis is expected to facilitate the discovery of the aberrant glycosyltransferases, N-glycosylation and glycoproteins in tumor progression and chemoresistance.					
34000456	12	93	theme	molecular	1994:2002	arg1	mechanisms					2004:2013	molecular mechanisms	1994:2013	molecular mechanisms underlying chemoresistance in BC	1994:2046	This study might provide valuable information to understand molecular mechanisms underlying chemoresistance in BC.					
34000456	12	94	from	chemoresistance	2026:2040	arg1	BC					2045:2046	BC	2045:2046	BC	2045:2046	This study might provide valuable information to understand molecular mechanisms underlying chemoresistance in BC.					
34000456	4	95	theme	cells	651:655	arg1	chemoresistance					618:632	chemoresistance	618:632	chemoresistance of breast cancer cells	618:655	In this study, an integrated strategy with a combination of transcriptomics, proteomics, glycomics and glycoproteomics was applied to explore the dysregulation of glycogenes, glycan structures and glycoproteins in chemoresistance of breast cancer cells.					
34000456	11	96	theme	unique	1832:1837	arg1	signatures					1887:1896	unique glycan-related protein, glycan and glycoprotein signatures	1832:1896	signatures	1887:1896	In this multi-omic analysis, we identified unique glycan-related protein, glycan and glycoprotein signatures defining PTX chemoresistance in BC.					
34000456	1	97	theme	deaths	239:244	arg1	rates					215:219	the high rates	206:219	the high rates of cancer-related deaths	206:244	Chemoresistance is a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths.					
34000456	1	97	theme	deaths	239:244	arg1	relapse					194:200	breast cancer (BC) relapse	175:200	breast cancer (BC) relapse	175:200	Chemoresistance is a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths.					
32024769	3	0	theme	several	752:758	arg1	cohorts					772:778	several independent cohorts	752:778	several independent cohorts	752:778	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	3	1	with	study	741:745	arg1	cohorts					772:778	several independent cohorts	752:778	several independent cohorts	752:778	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	3	1	with	study	741:745	arg1	individuals					795:805	over 3,000 individuals	784:805	over 3,000 individuals in total	784:814	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	0	2	theme	Cell-mediated	77:89	arg1	Cytotoxicity					91:102	Antibody-dependent Cell-mediated Cytotoxicity	58:102	Antibody-dependent Cell-mediated Cytotoxicity	58:102	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	3	3	theme	case-control	728:739	arg1	study					741:745	a case-control study	726:745	a case-control study with several independent cohorts and over 3,000 individuals in total	726:814	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	2	4	theme	distinctive	456:466	arg1	patterns					482:489	distinctive glycosylation patterns	456:489	distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions	456:563	Immunoglobulins and cell-surface receptors are glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions.					
32024769	3	5	dep	IgG	616:618	arg1	glycosylation					659:671	glycosylation	659:671	glycosylation	659:671	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	1	6	theme	lymphocyte	254:263	arg1	infiltration					265:276	lymphocyte infiltration	254:276	lymphocyte infiltration	254:276	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	4	7	theme	antennary	961:969	arg1	fucosylation					976:987	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation	916:987	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD	916:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	5	8	theme	antibody-dependent	1170:1187	arg1	cytotoxicity					1203:1214	antibody-dependent cell-mediated cytotoxicity	1170:1214	antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels	1170:1254	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	4	9	theme	decreased	916:924	arg1	fucosylation					976:987	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation	916:987	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD	916:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	3	10	from	associations	582:593	arg1	study					741:745	a case-control study	726:745	a case-control study with several independent cohorts and over 3,000 individuals in total	726:814	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	0	11	from	Player	48:53	arg1	Cytotoxicity					91:102	Antibody-dependent Cell-mediated Cytotoxicity	58:102	Antibody-dependent Cell-mediated Cytotoxicity	58:102	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	3	12	from	influence	691:699	arg1	study					741:745	a case-control study	726:745	a case-control study with several independent cohorts and over 3,000 individuals in total	726:814	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	5	13	theme	risk	1135:1138	arg1	factor					1140:1145	a risk factor	1133:1145	a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels	1133:1254	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	5	13	theme	risk	1135:1138	arg1	level					1099:1103	the decreased level	1085:1103	the decreased level of IgG core fucosylation	1085:1128	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	4	14	with	fucosylation	976:987	arg1	AITD					899:902	AITD	899:902	AITD	899:902	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	4	14	with	fucosylation	976:987	arg1	TPOAb					889:893	TPOAb	889:893	TPOAb	889:893	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	3	15	theme	independent	760:770	arg1	cohorts					772:778	several independent cohorts	752:778	several independent cohorts	752:778	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	3	16	from	individuals	795:805	arg1	total					810:814	total	810:814	total	810:814	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	2	17	theme	glycosylation	468:480	arg1	patterns					482:489	distinctive glycosylation patterns	456:489	distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions	456:563	Immunoglobulins and cell-surface receptors are glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions.					
32024769	0	18	theme	Immunoglobulin	10:23	arg1	Fucosylation					32:43	Decreased Immunoglobulin G Core Fucosylation	0:43	Decreased Immunoglobulin G Core Fucosylation	0:43	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	0	18	theme	Immunoglobulin	10:23	arg1	Player					48:53	A Player	46:53	A Player in Antibody-dependent Cell-mediated Cytotoxicity	46:102	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	2	19	theme	structural	503:512	arg1	role					514:517	a structural role	501:517	a structural role	501:517	Immunoglobulins and cell-surface receptors are glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions.					
32024769	4	20	theme	genetic	1012:1018	arg1	variance					1020:1027	genetic variance	1012:1027	genetic variance between AITD and glycosylation	1012:1058	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	3	21	theme	circulating	604:614	arg1	IgG					616:618	total circulating IgG	598:618	total circulating IgG	598:618	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	4	22	theme	peripheral	926:935	arg1	cells					955:959	peripheral blood mononuclear cells	926:959	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD	916:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	0	23	theme	Decreased	0:8	arg1	Fucosylation					32:43	Decreased Immunoglobulin G Core Fucosylation	0:43	Decreased Immunoglobulin G Core Fucosylation	0:43	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	0	23	theme	Decreased	0:8	arg1	Player					48:53	A Player	46:53	A Player in Antibody-dependent Cell-mediated Cytotoxicity	46:102	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	3	24	theme	IgG	616:618	arg1	influence					691:699	the influence	687:699	the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total	687:814	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	3	24	theme	IgG	616:618	arg1	associations					582:593	associations	582:593	associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD	582:681	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	5	25	theme	core	1112:1115	arg1	fucosylation					1117:1128	IgG core fucosylation	1108:1128	IgG core fucosylation	1108:1128	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	0	26	theme	Core	27:30	arg1	Fucosylation					32:43	Decreased Immunoglobulin G Core Fucosylation	0:43	Decreased Immunoglobulin G Core Fucosylation	0:43	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	0	26	theme	Core	27:30	arg1	Player					48:53	A Player	46:53	A Player in Antibody-dependent Cell-mediated Cytotoxicity	46:102	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	3	27	theme	cells	653:657	arg1	influence					691:699	the influence	687:699	the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total	687:814	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	3	27	theme	cells	653:657	arg1	associations					582:593	associations	582:593	associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD	582:681	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	0	28	theme	Thyroid	135:141	arg1	Diseases					143:150	Autoimmune Thyroid Diseases	124:150	Autoimmune Thyroid Diseases	124:150	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	4	29	theme	fucosylation	871:882	arg1	association					847:857	an inverse association	836:857	an inverse association of IgG core fucosylation with TPOAb and AITD	836:902	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	4	29	theme	fucosylation	871:882	arg1	fucosylation					976:987	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation	916:987	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD	916:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	4	30	theme	core	866:869	arg1	fucosylation					871:882	IgG core fucosylation	862:882	IgG core fucosylation	862:882	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	0	31	theme	G	25:25	arg1	Fucosylation					32:43	Decreased Immunoglobulin G Core Fucosylation	0:43	Decreased Immunoglobulin G Core Fucosylation	0:43	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	0	31	theme	G	25:25	arg1	Player					48:53	A Player	46:53	A Player in Antibody-dependent Cell-mediated Cytotoxicity	46:102	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	0	32	theme	Autoimmune	124:133	arg1	Diseases					143:150	Autoimmune Thyroid Diseases	124:150	Autoimmune Thyroid Diseases	124:150	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	1	33	theme	thyroid	375:381	arg1	gland					383:387	the thyroid gland	371:387	the thyroid gland	371:387	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	3	34	theme	total	598:602	arg1	IgG					616:618	total circulating IgG	598:618	total circulating IgG	598:618	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	3	35	with	influence	691:699	arg1	AITD					678:681	AITD	678:681	AITD	678:681	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	1	36	theme	common	201:206	arg1	diseases					172:179	Autoimmune thyroid diseases	153:179	Autoimmune thyroid diseases (AITD)	153:186	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	36	theme	common	201:206	arg1	group					208:212	the most common group	192:212	the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland	192:387	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	36	theme	common	201:206	arg1	diseases					228:235	autoimmune diseases	217:235	autoimmune diseases	217:235	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	37	theme	thyroid	300:306	arg1	autoantibodies					308:321	thyroid autoantibodies	300:321	thyroid autoantibodies	300:321	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	4	38	from	fucosylation	976:987	arg1	AITD					992:995	AITD	992:995	AITD	992:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	3	39	theme	blood	635:639	arg1	cells					653:657	peripheral blood mononuclear cells	624:657	peripheral blood mononuclear cells	624:657	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	5	40	theme	decreased	1089:1097	arg1	level					1099:1103	the decreased level	1085:1103	the decreased level of IgG core fucosylation	1085:1128	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	5	40	theme	decreased	1089:1097	arg1	factor					1140:1145	a risk factor	1133:1145	a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels	1133:1254	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	2	41	theme	cell-surface	410:421	arg1	glycoproteins					437:449	glycoproteins	437:449	glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions	437:563	Immunoglobulins and cell-surface receptors are glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions.					
32024769	2	41	theme	cell-surface	410:421	arg1	Immunoglobulins					390:404	Immunoglobulins	390:404	Immunoglobulins	390:404	Immunoglobulins and cell-surface receptors are glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions.					
32024769	2	41	theme	cell-surface	410:421	arg1	receptors					423:431	cell-surface receptors	410:431	cell-surface receptors	410:431	Immunoglobulins and cell-surface receptors are glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions.					
32024769	1	42	theme	autoantibodies	308:321	arg1	infiltration					265:276	lymphocyte infiltration	254:276	lymphocyte infiltration	254:276	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	42	theme	autoantibodies	308:321	arg1	production					286:295	the production	282:295	the production of thyroid autoantibodies	282:321	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	4	43	with	association	847:857	arg1	AITD					899:902	AITD	899:902	AITD	899:902	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	4	43	with	association	847:857	arg1	TPOAb					889:893	TPOAb	889:893	TPOAb	889:893	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	5	44	theme	fucosylation	1117:1128	arg1	level					1099:1103	the decreased level	1085:1103	the decreased level of IgG core fucosylation	1085:1128	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	5	44	theme	fucosylation	1117:1128	arg1	factor					1140:1145	a risk factor	1133:1145	a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels	1133:1254	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	3	45	theme	mononuclear	641:651	arg1	cells					653:657	peripheral blood mononuclear cells	624:657	peripheral blood mononuclear cells	624:657	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	1	46	from	group	208:212	arg1	gland					383:387	the thyroid gland	371:387	the thyroid gland	371:387	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	5	47	theme	IgG	1108:1110	arg1	fucosylation					1117:1128	IgG core fucosylation	1108:1128	IgG core fucosylation	1108:1128	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	5	48	theme	cell-mediated	1189:1201	arg1	cytotoxicity					1203:1214	antibody-dependent cell-mediated cytotoxicity	1170:1214	antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels	1170:1254	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	4	49	theme	blood	937:941	arg1	cells					955:959	peripheral blood mononuclear cells	926:959	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD	916:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	5	50	theme	TPOAb	1243:1247	arg1	levels					1249:1254	TPOAb levels	1243:1254	TPOAb levels	1243:1254	These data suggest that the decreased level of IgG core fucosylation is a risk factor for AITD that promotes antibody-dependent cell-mediated cytotoxicity previously associated with TPOAb levels.					
32024769	2	51	with	glycoproteins	437:449	arg1	patterns					482:489	distinctive glycosylation patterns	456:489	distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions	456:563	Immunoglobulins and cell-surface receptors are glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions.					
32024769	3	52	with	associations	582:593	arg1	AITD					678:681	AITD	678:681	AITD	678:681	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	4	53	theme	α1,2	971:974	arg1	fucosylation					976:987	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation	916:987	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD	916:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	3	54	theme	peripheral	624:633	arg1	cells					653:657	peripheral blood mononuclear cells	624:657	peripheral blood mononuclear cells	624:657	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	1	55	theme	thyroid	329:335	arg1	TPOAb					360:364	TPOAb	360:364	TPOAb	360:364	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	55	theme	thyroid	329:335	arg1	antibodies					348:357	thyroid peroxidase antibodies	329:357	thyroid peroxidase antibodies (TPOAb)	329:365	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	4	56	theme	cells	955:959	arg1	fucosylation					976:987	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation	916:987	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD	916:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	3	57	from	cohorts	772:778	arg1	total					810:814	total	810:814	total	810:814	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	4	58	theme	inverse	839:845	arg1	association					847:857	an inverse association	836:857	an inverse association of IgG core fucosylation with TPOAb and AITD	836:902	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	4	59	theme	IgG	862:864	arg1	fucosylation					871:882	IgG core fucosylation	862:882	IgG core fucosylation	862:882	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	4	60	gly	fucosylation	976:987	arg1	AITD					992:995	AITD	992:995	AITD	992:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	4	60	gly	fucosylation	976:987	arg1	fucosylation					871:882	IgG core fucosylation	862:882	IgG core fucosylation	862:882	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	1	61	theme	autoimmune	217:226	arg1	diseases					228:235	autoimmune diseases	217:235	autoimmune diseases	217:235	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	4	62	theme	mononuclear	943:953	arg1	cells					955:959	peripheral blood mononuclear cells	926:959	decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD	916:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	3	63	theme	genetic	704:710	arg1	background					712:721	genetic background	704:721	genetic background	704:721	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	1	64	theme	diseases	228:235	arg1	diseases					172:179	Autoimmune thyroid diseases	153:179	Autoimmune thyroid diseases (AITD)	153:186	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	64	theme	diseases	228:235	arg1	group					208:212	the most common group	192:212	the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland	192:387	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	64	theme	diseases	228:235	arg1	diseases					228:235	autoimmune diseases	217:235	autoimmune diseases	217:235	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	0	65	theme	Antibody-dependent	58:75	arg1	Cytotoxicity					91:102	Antibody-dependent Cell-mediated Cytotoxicity	58:102	Antibody-dependent Cell-mediated Cytotoxicity	58:102	Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent Cell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid Diseases.					
32024769	3	66	theme	background	712:721	arg1	influence					691:699	the influence	687:699	the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total	687:814	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	3	66	theme	background	712:721	arg1	associations					582:593	associations	582:593	associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD	582:681	We investigated associations of total circulating IgG and peripheral blood mononuclear cells glycosylation with AITD and the influence of genetic background in a case-control study with several independent cohorts and over 3,000 individuals in total.					
32024769	2	67	gly	glycoproteins	437:449	arg1	glycoproteins					437:449	glycoproteins	437:449	glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions	437:563	Immunoglobulins and cell-surface receptors are glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions.					
32024769	2	67	gly	glycoproteins	437:449	arg1	Immunoglobulins					390:404	Immunoglobulins	390:404	Immunoglobulins	390:404	Immunoglobulins and cell-surface receptors are glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions.					
32024769	2	67	gly	glycoproteins	437:449	arg1	receptors					423:431	cell-surface receptors	410:431	cell-surface receptors	410:431	Immunoglobulins and cell-surface receptors are glycoproteins with distinctive glycosylation patterns that play a structural role in maintaining and modulating their functions.					
32024769	4	68	from	association	847:857	arg1	AITD					992:995	AITD	992:995	AITD	992:995	The study revealed an inverse association of IgG core fucosylation with TPOAb and AITD, as well as decreased peripheral blood mononuclear cells antennary α1,2 fucosylation in AITD, but no shared genetic variance between AITD and glycosylation.					
32024769	1	69	theme	Autoimmune	153:162	arg1	group					208:212	the most common group	192:212	the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland	192:387	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	69	theme	Autoimmune	153:162	arg1	AITD					182:185	AITD	182:185	AITD	182:185	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	69	theme	Autoimmune	153:162	arg1	diseases					172:179	Autoimmune thyroid diseases	153:179	Autoimmune thyroid diseases (AITD)	153:186	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	69	theme	Autoimmune	153:162	arg1	diseases					228:235	autoimmune diseases	217:235	autoimmune diseases	217:235	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	70	theme	peroxidase	337:346	arg1	TPOAb					360:364	TPOAb	360:364	TPOAb	360:364	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	70	theme	peroxidase	337:346	arg1	antibodies					348:357	thyroid peroxidase antibodies	329:357	thyroid peroxidase antibodies (TPOAb)	329:365	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	71	theme	thyroid	164:170	arg1	group					208:212	the most common group	192:212	the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland	192:387	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	71	theme	thyroid	164:170	arg1	AITD					182:185	AITD	182:185	AITD	182:185	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	71	theme	thyroid	164:170	arg1	diseases					172:179	Autoimmune thyroid diseases	153:179	Autoimmune thyroid diseases (AITD)	153:186	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
32024769	1	71	theme	thyroid	164:170	arg1	diseases					228:235	autoimmune diseases	217:235	autoimmune diseases	217:235	Autoimmune thyroid diseases (AITD) are the most common group of autoimmune diseases, associated with lymphocyte infiltration and the production of thyroid autoantibodies, like thyroid peroxidase antibodies (TPOAb), in the thyroid gland.					
34791559	1	0	theme	villi	294:298	arg1	analysis					195:202	a comparative analysis	181:202	a comparative analysis of the expression of fucosylated glycans	181:243	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	1	0	theme	villi	294:298	arg1	characteristics					262:276	morphometric characteristics	249:276	morphometric characteristics of the terminal villi of the placenta	249:314	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	2	1	theme	residues	633:640	arg1	predominance					562:573	mild and severe PE: predominance	542:573	mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans	542:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	4	2	theme	severe	1145:1150	arg1	PE					1152:1153	severe PE	1145:1153	severe PE	1145:1153	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	3	3	theme	fucosylated	721:731	arg1	glycans					733:739	fucosylated glycans	721:739	fucosylated glycans	721:739	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	5	4	theme	villi	1428:1432	arg1	expression					1458:1467	the expression	1454:1467	the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE	1454:1539	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	5	4	theme	villi	1428:1432	arg1	characteristics					1396:1410	the morphometric characteristics	1379:1410	the morphometric characteristics of the terminal villi of the placenta	1379:1448	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	1	5	theme	comparative	183:193	arg1	analysis					195:202	a comparative analysis	181:202	a comparative analysis of the expression of fucosylated glycans	181:243	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	2	6	theme	clustered	616:624	arg1	residues					633:640	clustered fucose residues	616:640	clustered fucose residues	616:640	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	2	7	with	residues	633:640	arg1	α1,6-fucose					591:601	α1,6-fucose	591:601	α1,6-fucose	591:601	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	1	8	theme	expression	211:220	arg1	analysis					195:202	a comparative analysis	181:202	a comparative analysis of the expression of fucosylated glycans	181:243	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	1	8	theme	expression	211:220	arg1	characteristics					262:276	morphometric characteristics	249:276	morphometric characteristics of the terminal villi of the placenta	249:314	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	2	9	theme	severe	551:556	arg1	predominance					562:573	mild and severe PE: predominance	542:573	mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans	542:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	6	10	theme	placental	1628:1636	arg1	structures					1646:1655	placental barrier structures	1628:1655	placental barrier structures	1628:1655	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	1	11	theme	fucosylated	225:235	arg1	glycans					237:243	fucosylated glycans	225:243	fucosylated glycans	225:243	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	6	12	theme	severity	1717:1724	arg1	parameters					1678:1687	the morphometric parameters	1661:1687	the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier	1661:1780	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	6	12	theme	severity	1717:1724	arg1	expression					1573:1582	a changed expression	1563:1582	a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures	1563:1655	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	3	13	theme	glycocalyx	780:789	arg1	pattern					710:716	The expression pattern	695:716	The expression pattern of fucosylated glycans	695:739	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	13	theme	glycocalyx	780:789	arg1	composition					749:759	the composition	745:759	the composition of the endothelial glycocalyx	745:789	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	13	theme	glycocalyx	780:789	arg1	pattern					828:834	expression pattern	817:834	expression pattern	817:834	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	0	14	theme	Preeclampsia	154:165	arg1	Severity					142:149	the Severity	138:149	the Severity of Preeclampsia	138:165	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	2	15	from	glycan	651:656	arg1	core					610:613	the core	606:613	the core	606:613	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	3	16	theme	expression	817:826	arg1	pattern					710:716	The expression pattern	695:716	The expression pattern of fucosylated glycans	695:739	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	16	theme	expression	817:826	arg1	composition					749:759	the composition	745:759	the composition of the endothelial glycocalyx	745:789	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	16	theme	expression	817:826	arg1	pattern					828:834	expression pattern	817:834	expression pattern	817:834	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	17	theme	syncytiotrophoblast	859:877	arg1	glycocalyx					879:888	the syncytiotrophoblast glycocalyx	855:888	the syncytiotrophoblast glycocalyx	855:888	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	2	18	theme	normal	516:521	arg1	pregnancy					523:531	normal pregnancy	516:531	normal pregnancy	516:531	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	4	19	theme	total	1160:1164	arg1	area					1175:1178	the total vascular area	1156:1178	the total vascular area in the villus	1156:1192	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	6	20	theme	fucosylated	1587:1597	arg1	glycans					1599:1605	fucosylated glycans	1587:1605	fucosylated glycans	1587:1605	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	3	21	theme	mild	902:905	arg1	PE					918:919	mild and severe PE	902:919	mild and severe PE	902:919	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	2	22	theme	patients	502:509	arg1	tissue					492:497	the placental tissue	478:497	the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans	478:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	6	23	theme	barrier	1774:1780	arg1	function					1748:1755	the function	1744:1755	the function of the placental barrier	1744:1780	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	2	24	with	patients	502:509	arg1	predominance					562:573	mild and severe PE: predominance	542:573	mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans	542:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	2	24	with	patients	502:509	arg1	pregnancy					523:531	normal pregnancy	516:531	normal pregnancy	516:531	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	2	25	theme	mild	542:545	arg1	predominance					562:573	mild and severe PE: predominance	542:573	mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans	542:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	2	26	gly	fucosylated	403:413	arg1	glycans					415:421	fucosylated glycans	403:421	fucosylated glycans	403:421	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	2	27	theme	LeX	647:649	arg1	glycan					651:656	LeX glycan	647:656	LeX glycan over α1,2-fucose-containing glycans	647:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	0	28	theme	Terminal	100:107	arg1	Villi					119:123	Terminal Placental Villi	100:123	Terminal Placental Villi	100:123	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	5	29	theme	fucosylated	1472:1482	arg1	glycans					1484:1490	fucosylated glycans	1472:1490	fucosylated glycans	1472:1490	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	5	30	theme	direct	1335:1340	arg1	relationships					1357:1369	direct and reciprocal relationships	1335:1369	direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE	1335:1539	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	0	31	theme	Villi	119:123	arg1	Parameters					86:95	Morphometric Parameters	73:95	Morphometric Parameters of Terminal Placental Villi	73:123	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	0	31	theme	Villi	119:123	arg1	Characteristics					12:26	Comparative Characteristics	0:26	Comparative Characteristics of the Expression of Fucosylated Glycans	0:67	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	3	32	theme	severe	911:916	arg1	PE					918:919	mild and severe PE	902:919	mild and severe PE	902:919	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	5	33	theme	reciprocal	1346:1355	arg1	relationships					1357:1369	direct and reciprocal relationships	1335:1369	direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE	1335:1539	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	0	34	theme	Comparative	0:10	arg1	Characteristics					12:26	Comparative Characteristics	0:26	Comparative Characteristics of the Expression of Fucosylated Glycans	0:67	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	6	35	theme	structures	1646:1655	arg1	glycocalyx					1614:1623	the glycocalyx	1610:1623	the glycocalyx of placental barrier structures	1610:1655	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	1	36	theme	placenta	307:314	arg1	villi					294:298	the terminal villi	281:298	the terminal villi of the placenta	281:314	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	3	37	theme	expression	926:935	arg1	pattern					937:943	the expression pattern	922:943	the expression pattern of fucosylated glycans	922:966	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	38	gly	fucosylated	721:731	arg1	glycans					733:739	fucosylated glycans	721:739	fucosylated glycans	721:739	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	4	39	theme	mild	1287:1290	arg1	PE					1292:1293	mild PE	1287:1293	mild PE (p=0.04)	1287:1302	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	4	39	theme	mild	1287:1290	arg1	p=0.04					1296:1301	p=0.04	1296:1301	p=0.04	1296:1301	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	2	40	theme	glycans	415:421	arg1	expression					389:398	the expression	385:398	the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx	385:459	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	0	41	theme	Expression	35:44	arg1	Parameters					86:95	Morphometric Parameters	73:95	Morphometric Parameters of Terminal Placental Villi	73:123	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	0	41	theme	Expression	35:44	arg1	Characteristics					12:26	Comparative Characteristics	0:26	Comparative Characteristics of the Expression of Fucosylated Glycans	0:67	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	3	42	theme	endothelial	768:778	arg1	glycocalyx					780:789	the endothelial glycocalyx	764:789	the endothelial glycocalyx	764:789	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	0	43	theme	Fucosylated	49:59	arg1	Glycans					61:67	Fucosylated Glycans	49:67	Fucosylated Glycans	49:67	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	4	44	with	patients	1131:1138	arg1	PE					1152:1153	severe PE	1145:1153	severe PE	1145:1153	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	3	45	theme	glycans	960:966	arg1	pattern					937:943	the expression pattern	922:943	the expression pattern of fucosylated glycans	922:966	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	5	46	theme	terminal	1419:1426	arg1	villi					1428:1432	the terminal villi	1415:1432	the terminal villi of the placenta	1415:1448	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	1	47	theme	glycans	237:243	arg1	expression					211:220	the expression	207:220	the expression of fucosylated glycans	207:243	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	4	48	from	area	1175:1178	arg1	villus					1187:1192	the villus	1183:1192	the villus	1183:1192	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	3	49	theme	α1,2-fucose-containing	995:1016	arg1	glycans					1018:1024	α1,2-fucose-containing glycans	995:1024	α1,2-fucose-containing glycans	995:1024	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	1	50	theme	morphometric	249:260	arg1	characteristics					262:276	morphometric characteristics	249:276	morphometric characteristics of the terminal villi of the placenta	249:314	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	5	51	theme	morphometric	1383:1394	arg1	characteristics					1396:1410	the morphometric characteristics	1379:1410	the morphometric characteristics of the terminal villi of the placenta	1379:1448	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	2	52	from	predominance	562:573	arg1	core					610:613	the core	606:613	the core	606:613	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	3	53	theme	expression	699:708	arg1	pattern					710:716	The expression pattern	695:716	The expression pattern of fucosylated glycans	695:739	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	53	theme	expression	699:708	arg1	composition					749:759	the composition	745:759	the composition of the endothelial glycocalyx	745:789	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	53	theme	expression	699:708	arg1	pattern					828:834	expression pattern	817:834	expression pattern	817:834	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	2	54	with	glycans	578:584	arg1	α1,6-fucose					591:601	α1,6-fucose	591:601	α1,6-fucose	591:601	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	1	55	theme	terminal	285:292	arg1	villi					294:298	the terminal villi	281:298	the terminal villi of the placenta	281:314	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	2	56	from	glycans	578:584	arg1	core					610:613	the core	606:613	the core	606:613	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	6	57	theme	changed	1565:1571	arg1	expression					1573:1582	a changed expression	1563:1582	a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures	1563:1655	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	6	58	from	parameters	1678:1687	arg1	glycocalyx					1614:1623	the glycocalyx	1610:1623	the glycocalyx of placental barrier structures	1610:1655	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	6	58	from	parameters	1678:1687	arg1	PE					1701:1702	PE	1701:1702	PE	1701:1702	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	2	59	theme	fucose	626:631	arg1	residues					633:640	clustered fucose residues	616:640	clustered fucose residues	616:640	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	3	60	theme	glycans	733:739	arg1	pattern					710:716	The expression pattern	695:716	The expression pattern of fucosylated glycans	695:739	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	60	theme	glycans	733:739	arg1	composition					749:759	the composition	745:759	the composition of the endothelial glycocalyx	745:789	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	60	theme	glycans	733:739	arg1	pattern					828:834	expression pattern	817:834	expression pattern	817:834	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	1	61	gly	fucosylated	225:235	arg1	glycans					237:243	fucosylated glycans	225:243	fucosylated glycans	225:243	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	2	62	from	patterns	373:380	arg1	glycocalyx					450:459	the syncytiotrophoblast glycocalyx	426:459	the syncytiotrophoblast glycocalyx	426:459	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	3	63	theme	endothelial	1057:1067	arg1	glycocalyx					1069:1078	the endothelial glycocalyx	1053:1078	the endothelial glycocalyx	1053:1078	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	5	64	theme	placenta	1441:1448	arg1	villi					1428:1432	the terminal villi	1415:1432	the terminal villi of the placenta	1415:1448	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	6	65	theme	villi	1692:1696	arg1	parameters					1678:1687	the morphometric parameters	1661:1687	the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier	1661:1780	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	6	65	theme	villi	1692:1696	arg1	expression					1573:1582	a changed expression	1563:1582	a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures	1563:1655	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	2	66	theme	PE	558:559	arg1	predominance					562:573	mild and severe PE: predominance	542:573	mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans	542:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	5	67	from	endothelium	1523:1533	arg1	PE					1538:1539	PE	1538:1539	PE	1538:1539	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	3	68	gly	fucosylated	948:958	arg1	glycans					960:966	fucosylated glycans	948:966	fucosylated glycans	948:966	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	69	dep	pattern	828:834	arg1	the					813:815	the	813:815	the	813:815	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	5	70	from	syncytiotrophoblast	1499:1517	arg1	PE					1538:1539	PE	1538:1539	PE	1538:1539	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	2	71	theme	glycans	578:584	arg1	predominance					562:573	mild and severe PE: predominance	542:573	mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans	542:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	6	72	theme	different	1707:1715	arg1	severity					1717:1724	different severity	1707:1724	different severity	1707:1724	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	4	73	theme	physiological	1240:1252	arg1	course					1254:1259	physiological course	1240:1259	physiological course of pregnancy (p=0.04) and mild PE (p=0.04)	1240:1302	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	4	74	theme	vascular	1166:1173	arg1	area					1175:1178	the total vascular area	1156:1178	the total vascular area in the villus	1156:1192	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	0	75	theme	Morphometric	73:84	arg1	Parameters					86:95	Morphometric Parameters	73:95	Morphometric Parameters of Terminal Placental Villi	73:123	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	6	76	theme	glycans	1599:1605	arg1	parameters					1678:1687	the morphometric parameters	1661:1687	the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier	1661:1780	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	6	76	theme	glycans	1599:1605	arg1	expression					1573:1582	a changed expression	1563:1582	a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures	1563:1655	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	3	77	theme	glycocalyx	879:888	arg1	pattern					710:716	The expression pattern	695:716	The expression pattern of fucosylated glycans	695:739	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	77	theme	glycocalyx	879:888	arg1	composition					840:850	composition	840:850	composition	840:850	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	77	theme	glycocalyx	879:888	arg1	composition					749:759	the composition	745:759	the composition of the endothelial glycocalyx	745:789	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	3	77	theme	glycocalyx	879:888	arg1	pattern					828:834	expression pattern	817:834	expression pattern	817:834	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	2	78	from	glycocalyx	450:459	arg1	patterns					373:380	Similar patterns	365:380	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx	365:459	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	2	79	with	glycan	651:656	arg1	α1,6-fucose					591:601	α1,6-fucose	591:601	α1,6-fucose	591:601	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	1	80	theme	preeclampsia	346:357	arg1	severity					334:341	the severity	330:341	the severity of preeclampsia (PE)	330:362	We performed a comparative analysis of the expression of fucosylated glycans and morphometric characteristics of the terminal villi of the placenta, depending on the severity of preeclampsia (PE).					
34791559	6	81	theme	placental	1764:1772	arg1	barrier					1774:1780	the placental barrier	1760:1780	the placental barrier	1760:1780	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	2	82	theme	glycan	651:656	arg1	predominance					562:573	mild and severe PE: predominance	542:573	mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans	542:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	0	83	theme	Placental	109:117	arg1	Villi					119:123	Terminal Placental Villi	100:123	Terminal Placental Villi	100:123	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	4	84	theme	pregnancy	1264:1272	arg1	course					1254:1259	physiological course	1240:1259	physiological course of pregnancy (p=0.04) and mild PE (p=0.04)	1240:1302	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	6	85	theme	morphometric	1665:1676	arg1	parameters					1678:1687	the morphometric parameters	1661:1687	the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier	1661:1780	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	6	86	from	expression	1573:1582	arg1	glycocalyx					1614:1623	the glycocalyx	1610:1623	the glycocalyx of placental barrier structures	1610:1655	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	6	86	from	expression	1573:1582	arg1	PE					1701:1702	PE	1701:1702	PE	1701:1702	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	5	87	theme	glycans	1484:1490	arg1	expression					1458:1467	the expression	1454:1467	the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE	1454:1539	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	5	87	theme	glycans	1484:1490	arg1	characteristics					1396:1410	the morphometric characteristics	1379:1410	the morphometric characteristics of the terminal villi of the placenta	1379:1448	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	2	88	theme	expression	389:398	arg1	patterns					373:380	Similar patterns	365:380	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx	365:459	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	2	89	theme	α1,2-fucose-containing	663:684	arg1	glycans					686:692	α1,2-fucose-containing glycans	663:692	α1,2-fucose-containing glycans	663:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	6	90	theme	barrier	1638:1644	arg1	structures					1646:1655	placental barrier structures	1628:1655	placental barrier structures	1628:1655	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
34791559	3	91	theme	fucosylated	948:958	arg1	glycans					960:966	fucosylated glycans	948:966	fucosylated glycans	948:966	The expression pattern of fucosylated glycans and the composition of the endothelial glycocalyx are normally close to the expression pattern and composition of the syncytiotrophoblast glycocalyx; in case of mild and severe PE, the expression pattern of fucosylated glycans was changed uniformly, and α1,2-fucose-containing glycans significantly prevailed in the endothelial glycocalyx.					
34791559	2	92	theme	syncytiotrophoblast	430:448	arg1	glycocalyx					450:459	the syncytiotrophoblast glycocalyx	426:459	the syncytiotrophoblast glycocalyx	426:459	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	2	93	from	core	610:613	arg1	predominance					562:573	mild and severe PE: predominance	542:573	mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans	542:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	4	94	theme	PE	1292:1293	arg1	course					1254:1259	physiological course	1240:1259	physiological course of pregnancy (p=0.04) and mild PE (p=0.04)	1240:1302	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	5	95	theme	Correlation	1305:1315	arg1	analysis					1317:1324	Correlation analysis	1305:1324	Correlation analysis	1305:1324	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	5	96	gly	fucosylated	1472:1482	arg1	glycans					1484:1490	fucosylated glycans	1472:1490	fucosylated glycans	1472:1490	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	5	97	from	characteristics	1396:1410	arg1	endothelium					1523:1533	endothelium	1523:1533	endothelium	1523:1533	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	5	97	from	characteristics	1396:1410	arg1	syncytiotrophoblast					1499:1517	syncytiotrophoblast	1499:1517	syncytiotrophoblast	1499:1517	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	2	98	theme	placental	482:490	arg1	tissue					492:497	the placental tissue	478:497	the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans	478:692	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	2	99	theme	fucosylated	403:413	arg1	glycans					415:421	fucosylated glycans	403:421	fucosylated glycans	403:421	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	0	100	theme	Glycans	61:67	arg1	Expression					35:44	the Expression	31:44	the Expression of Fucosylated Glycans	31:67	Comparative Characteristics of the Expression of Fucosylated Glycans and Morphometric Parameters of Terminal Placental Villi Depending on the Severity of Preeclampsia.					
34791559	2	101	from	residues	633:640	arg1	core					610:613	the core	606:613	the core	606:613	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	2	102	from	expression	389:398	arg1	glycocalyx					450:459	the syncytiotrophoblast glycocalyx	426:459	the syncytiotrophoblast glycocalyx	426:459	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	5	103	from	expression	1458:1467	arg1	endothelium					1523:1533	endothelium	1523:1533	endothelium	1523:1533	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	5	103	from	expression	1458:1467	arg1	syncytiotrophoblast					1499:1517	syncytiotrophoblast	1499:1517	syncytiotrophoblast	1499:1517	Correlation analysis revealed direct and reciprocal relationships between the morphometric characteristics of the terminal villi of the placenta and the expression of fucosylated glycans in the syncytiotrophoblast and endothelium in PE.					
34791559	4	104	theme	LSD	1118:1120	arg1	test					1122:1125	Fisher's LSD test	1109:1125	Fisher's LSD test	1109:1125	According to the results of Fisher's LSD test, in patients with severe PE, the total vascular area in the villus prevailed over the indices established during physiological course of pregnancy (p=0.04) and mild PE (p=0.04).					
34791559	2	105	theme	Similar	365:371	arg1	patterns					373:380	Similar patterns	365:380	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx	365:459	Similar patterns of the expression of fucosylated glycans in the syncytiotrophoblast glycocalyx were revealed in the placental tissue of patients with normal pregnancy and with mild and severe PE: predominance of glycans with α1,6-fucose in the core, clustered fucose residues, and LeX glycan over α1,2-fucose-containing glycans.					
34791559	6	106	gly	fucosylated	1587:1597	arg1	glycans					1599:1605	fucosylated glycans	1587:1605	fucosylated glycans	1587:1605	Our results indicate a changed expression of fucosylated glycans in the glycocalyx of placental barrier structures and the morphometric parameters of villi in PE of different severity, which can affect the function of the placental barrier.					
33241822	2	0	dep	electrophoresis	544:558	arg1	analyses					673:680	analyses	673:680	analyses	673:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	1	1	contain	has	167:169	arg1	Glycosylation					153:165	Glycosylation	153:165	Glycosylation	153:165	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	1	1	contain	has	167:169	arg2	effect					179:184	a great effect	171:184	a great effect	171:184	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	1	2	theme	antioxidant	193:203	arg1	ability					205:211	the antioxidant ability	189:211	the antioxidant ability	189:211	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	1	2	theme	antioxidant	193:203	arg1	due					235:237	due	235:237	due	235:237	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	2	3	dep	transform	616:624	arg1	infrared					626:633	infrared	626:633	transform infrared (FT-IR)	616:641	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	1	4	theme	peptides	282:289	arg1	change					272:277	the structural conformational change	242:277	the structural conformational change of peptides in the protein	242:304	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	0	5	theme	mass	134:137	arg1	spectroscopy					139:150	high-resolution mass spectroscopy	118:150	high-resolution mass spectroscopy	118:150	Glycation mechanism of lactoferrin-chitosan oligosaccharide conjugates with improved antioxidant activity revealed by high-resolution mass spectroscopy.					
33241822	2	6	with	glycation	390:398	arg1	LF					418:419	LF	418:419	LF	418:419	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	6	with	glycation	390:398	arg1	lactoferrin					405:415	lactoferrin	405:415	lactoferrin (LF)	405:420	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	7	dep	Fourier	608:614	arg1	transform					616:624	transform	616:624	transform infrared (FT-IR)	616:641	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	3	8	theme	ABTS	791:794	arg1	assays					842:847	The ABTS and oxygen radical absorbance capacity (ORAC) assays	787:847	The ABTS and oxygen radical absorbance capacity (ORAC) assays	787:847	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	0	9	theme	high-resolution	118:132	arg1	spectroscopy					139:150	high-resolution mass spectroscopy	118:150	high-resolution mass spectroscopy	118:150	Glycation mechanism of lactoferrin-chitosan oligosaccharide conjugates with improved antioxidant activity revealed by high-resolution mass spectroscopy.					
33241822	2	10	from	electrophoresis	544:558	arg1	bond					591:594	the bond	587:594	the bond	587:594	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	4	11	theme	conformational	1088:1101	arg1	change					1103:1108	structural conformational change	1077:1108	structural conformational change of peptides in lactoferrin	1077:1135	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	2	12	theme	chitosan	712:719	arg1	oligosaccharide					721:735	the chitosan oligosaccharide	708:735	the chitosan oligosaccharide	708:735	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	13	theme	molecular	473:481	arg1	zone					483:486	a higher molecular zone	464:486	a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	464:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	14	theme	wet-heat	427:434	arg1	method					436:441	a wet-heat method	425:441	a wet-heat method	425:441	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	15	used	used	687:690	arg2	stripe					454:459	a new stripe	448:459	a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	448:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	15	used	used	687:690	arg2	zone					483:486	a higher molecular zone	464:486	a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	464:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	4	16	theme	enhanced	1043:1050	arg1	capacity					1064:1071	enhanced antioxidant capacity	1043:1071	enhanced antioxidant capacity	1043:1071	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	4	17	theme	mass	985:988	arg1	spectroscopy					990:1001	High-resolution mass spectroscopy	969:1001	High-resolution mass spectroscopy	969:1001	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	4	18	theme	High-resolution	969:983	arg1	spectroscopy					990:1001	High-resolution mass spectroscopy	969:1001	High-resolution mass spectroscopy	969:1001	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	2	19	theme	chitosan	324:331	arg1	COS					350:352	COS	350:352	COS	350:352	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	19	theme	chitosan	324:331	arg1	oligosaccharide					333:347	a chitosan oligosaccharide	322:347	a chitosan oligosaccharide (COS)	322:353	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	19	theme	chitosan	324:331	arg1	saccharide					375:384	the saccharide	371:384	the saccharide for glycation with lactoferrin (LF)	371:420	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	20	theme	electrophoresis	544:558	arg1	gel					495:497	the gel	491:497	the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	491:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	1	21	theme	proteins	216:223	arg1	ability					205:211	the antioxidant ability	189:211	the antioxidant ability	189:211	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	1	21	theme	proteins	216:223	arg1	due					235:237	due	235:237	due	235:237	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	0	22	theme	Glycation	0:8	arg1	mechanism					10:18	Glycation mechanism	0:18	Glycation mechanism of lactoferrin-chitosan oligosaccharide	0:58	Glycation mechanism of lactoferrin-chitosan oligosaccharide conjugates with improved antioxidant activity revealed by high-resolution mass spectroscopy.					
33241822	4	23	from	capacity	1064:1071	arg1	lactoferrin					1125:1135	lactoferrin	1125:1135	lactoferrin	1125:1135	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	2	24	theme	change	577:582	arg1	gel					495:497	the gel	491:497	the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	491:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	25	from	spectroscopy	660:671	arg1	bond					591:594	the bond	587:594	the bond	587:594	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	0	26	theme	lactoferrin-chitosan	23:42	arg1	oligosaccharide					44:58	lactoferrin-chitosan oligosaccharide	23:58	lactoferrin-chitosan oligosaccharide	23:58	Glycation mechanism of lactoferrin-chitosan oligosaccharide conjugates with improved antioxidant activity revealed by high-resolution mass spectroscopy.					
33241822	2	27	theme	dodecyl	509:515	arg1	SDS-PAGE					561:568	SDS-PAGE	561:568	SDS-PAGE	561:568	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	27	theme	dodecyl	509:515	arg1	electrophoresis					544:558	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	502:558	sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	502:569	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	4	28	theme	antioxidant	1052:1062	arg1	capacity					1064:1071	enhanced antioxidant capacity	1043:1071	enhanced antioxidant capacity	1043:1071	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	2	29	theme	sodium	502:507	arg1	SDS-PAGE					561:568	SDS-PAGE	561:568	SDS-PAGE	561:568	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	29	theme	sodium	502:507	arg1	electrophoresis					544:558	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	502:558	sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	502:569	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	3	30	theme	antioxidant	897:907	arg1	activity					909:916	a stronger antioxidant activity	886:916	a stronger antioxidant activity	886:916	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	2	31	theme	new	450:452	arg1	stripe					454:459	a new stripe	448:459	a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	448:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	3	32	theme	stronger	888:895	arg1	activity					909:916	a stronger antioxidant activity	886:916	a stronger antioxidant activity	886:916	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	4	33	theme	change	1103:1108	arg1	relationship					1027:1038	the relationship	1023:1038	the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin	1023:1135	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	4	34	theme	molecular	1142:1150	arg1	level					1152:1156	a molecular level	1140:1156	a molecular level	1140:1156	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	0	35	theme	oligosaccharide	44:58	arg1	mechanism					10:18	Glycation mechanism	0:18	Glycation mechanism of lactoferrin-chitosan oligosaccharide	0:58	Glycation mechanism of lactoferrin-chitosan oligosaccharide conjugates with improved antioxidant activity revealed by high-resolution mass spectroscopy.					
33241822	4	36	from	relationship	1027:1038	arg1	lactoferrin					1125:1135	lactoferrin	1125:1135	lactoferrin	1125:1135	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	3	37	theme	radical	807:813	arg1	ORAC					836:839	ORAC	836:839	ORAC	836:839	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	3	37	theme	radical	807:813	arg1	capacity					826:833	oxygen radical absorbance capacity	800:833	oxygen radical absorbance capacity (ORAC)	800:840	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	4	38	theme	capacity	1064:1071	arg1	relationship					1027:1038	the relationship	1023:1038	the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin	1023:1135	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	3	39	theme	chitosan	943:950	arg1	oligosaccharide					952:966	the chitosan oligosaccharide	939:966	the chitosan oligosaccharide	939:966	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	0	40	theme	improved	76:83	arg1	activity					97:104	improved antioxidant activity	76:104	improved antioxidant activity revealed by high-resolution mass spectroscopy	76:150	Glycation mechanism of lactoferrin-chitosan oligosaccharide conjugates with improved antioxidant activity revealed by high-resolution mass spectroscopy.					
33241822	2	41	from	change	577:582	arg1	bond					591:594	the bond	587:594	the bond	587:594	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	3	42	with	glycation	924:932	arg1	oligosaccharide					952:966	the chitosan oligosaccharide	939:966	the chitosan oligosaccharide	939:966	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	2	43	theme	spectroscopy	660:671	arg1	gel					495:497	the gel	491:497	the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	491:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	4	44	from	lactoferrin	1125:1135	arg1	relationship					1027:1038	the relationship	1023:1038	the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin	1023:1135	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	2	45	from	bond	591:594	arg1	gel					495:497	the gel	491:497	the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	491:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	1	46	from	change	272:277	arg1	protein					298:304	the protein	294:304	the protein	294:304	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	2	47	from	zone	483:486	arg1	stripe					454:459	a new stripe	448:459	a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	448:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	47	from	zone	483:486	arg1	gel					495:497	the gel	491:497	the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	491:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	48	theme	fluorescence	647:658	arg1	spectroscopy					660:671	fluorescence spectroscopy	647:671	fluorescence spectroscopy	647:671	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	4	49	theme	peptides	1113:1120	arg1	change					1103:1108	structural conformational change	1077:1108	structural conformational change of peptides in lactoferrin	1077:1135	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	4	49	theme	peptides	1113:1120	arg1	capacity					1064:1071	enhanced antioxidant capacity	1043:1071	enhanced antioxidant capacity	1043:1071	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	2	50	theme	gel	540:542	arg1	SDS-PAGE					561:568	SDS-PAGE	561:568	SDS-PAGE	561:568	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	50	theme	gel	540:542	arg1	electrophoresis					544:558	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	502:558	sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	502:569	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	51	dep	infrared	626:633	arg1	FT-IR					636:640	FT-IR	636:640	FT-IR	636:640	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	52	theme	sulfate-polyacrylamide	517:538	arg1	SDS-PAGE					561:568	SDS-PAGE	561:568	SDS-PAGE	561:568	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	2	52	theme	sulfate-polyacrylamide	517:538	arg1	electrophoresis					544:558	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	502:558	sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	502:569	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	1	53	theme	great	173:177	arg1	effect					179:184	a great effect	171:184	a great effect	171:184	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	3	54	theme	absorbance	815:824	arg1	ORAC					836:839	ORAC	836:839	ORAC	836:839	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	3	54	theme	absorbance	815:824	arg1	capacity					826:833	oxygen radical absorbance capacity	800:833	oxygen radical absorbance capacity (ORAC)	800:840	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	1	55	theme	structural	246:255	arg1	change					272:277	the structural conformational change	242:277	the structural conformational change of peptides in the protein	242:304	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	2	56	theme	higher	466:471	arg1	zone					483:486	a higher molecular zone	464:486	a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses	464:680	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	3	57	dep	radical	807:813	arg1	oxygen					800:805	oxygen	800:805	oxygen	800:805	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	4	58	from	change	1103:1108	arg1	lactoferrin					1125:1135	lactoferrin	1125:1135	lactoferrin	1125:1135	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
33241822	3	59	theme	capacity	826:833	arg1	assays					842:847	The ABTS and oxygen radical absorbance capacity (ORAC) assays	787:847	The ABTS and oxygen radical absorbance capacity (ORAC) assays	787:847	The ABTS and oxygen radical absorbance capacity (ORAC) assays indicated that lactoferrin exhibited a stronger antioxidant activity after glycation with the chitosan oligosaccharide.					
33241822	1	60	theme	conformational	257:270	arg1	change					272:277	the structural conformational change	242:277	the structural conformational change of peptides in the protein	242:304	Glycosylation has a great effect on the antioxidant ability of proteins, which is due to the structural conformational change of peptides in the protein.					
33241822	0	61	theme	antioxidant	85:95	arg1	activity					97:104	improved antioxidant activity	76:104	improved antioxidant activity revealed by high-resolution mass spectroscopy	76:150	Glycation mechanism of lactoferrin-chitosan oligosaccharide conjugates with improved antioxidant activity revealed by high-resolution mass spectroscopy.					
33241822	2	62	from	gel	495:497	arg1	bond					591:594	the bond	587:594	the bond	587:594	In this study, a chitosan oligosaccharide (COS) was selected as the saccharide for glycation with lactoferrin (LF) by a wet-heat method, and a new stripe at a higher molecular zone in the gel of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a change in the bond revealed by Fourier transform infrared (FT-IR) and fluorescence spectroscopy analyses were used to confirm that the chitosan oligosaccharide was successfully covalently bound to lactoferrin.					
33241822	4	63	theme	structural	1077:1086	arg1	change					1103:1108	structural conformational change	1077:1108	structural conformational change of peptides in lactoferrin	1077:1135	High-resolution mass spectroscopy further illuminated the relationship of enhanced antioxidant capacity and structural conformational change of peptides in lactoferrin at a molecular level.					
34367227	6	0	theme	many	976:979	arg1	cases					981:985	many cases	976:985	many cases	976:985	In many cases it may be necessary to reproduce features of their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens.					
34367227	4	1	theme	important	654:662	arg1	glycoproteins					636:648	Viral envelope glycoproteins	621:648	Viral envelope glycoproteins	621:648	Viral envelope glycoproteins are important targets for immunization, yet in some cases they accumulate poorly in plants and may not be properly processed.					
34367227	4	1	theme	important	654:662	arg1	targets					664:670	important targets	654:670	important targets for immunization	654:687	Viral envelope glycoproteins are important targets for immunization, yet in some cases they accumulate poorly in plants and may not be properly processed.					
34367227	7	2	theme	model	1246:1250	arg1	glycoproteins					1252:1264	model glycoproteins	1246:1264	model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells	1246:1336	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	9	3	theme	equivalent	1548:1557	arg1	proteins					1582:1589	the equivalent mammalian cell-derived proteins	1544:1589	the equivalent mammalian cell-derived proteins	1544:1589	Both glycoproteins yielded increased amounts of protein aggregates when produced in plants compared to the equivalent mammalian cell-derived proteins.					
34367227	9	4	theme	cell-derived	1569:1580	arg1	proteins					1582:1589	the equivalent mammalian cell-derived proteins	1544:1589	the equivalent mammalian cell-derived proteins	1544:1589	Both glycoproteins yielded increased amounts of protein aggregates when produced in plants compared to the equivalent mammalian cell-derived proteins.					
34367227	10	5	theme	lower	1727:1731	arg1	levels					1733:1738	lower levels	1727:1738	lower levels of glycan occupancy	1727:1758	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	3	6	theme	pharmaceuticals	604:618	arg1	production					582:591	the production	578:591	the production of complex pharmaceuticals	578:618	However, there are several differences along the plant protein secretory pathway compared to mammalian systems, which constrain the production of complex pharmaceuticals.					
34367227	1	7	theme	biologics	227:235	arg1	production					237:246	biologics production	227:246	biologics production	227:246	There is an urgent need to establish large scale biopharmaceutical manufacturing capacity in Africa where the infrastructure for biologics production is severely limited.					
34367227	11	8	gly	glycosylation	1871:1883	arg1	glycoproteins					1894:1906	viral glycoproteins	1888:1906	viral glycoproteins produced in N. benthamiana	1888:1933	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	10	9	theme	reduced	1761:1767	arg1	glycans					1777:1783	reduced complex glycans	1761:1783	reduced complex glycans	1761:1783	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	10	theme	occupancy	1750:1758	arg1	glycans					1777:1783	reduced complex glycans	1761:1783	reduced complex glycans	1761:1783	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	10	theme	occupancy	1750:1758	arg1	amounts					1795:1801	large amounts	1789:1801	large amounts of paucimannosidic structures	1789:1831	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	10	theme	occupancy	1750:1758	arg1	structures					1822:1831	paucimannosidic structures	1806:1831	paucimannosidic structures	1806:1831	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	10	theme	occupancy	1750:1758	arg1	levels					1733:1738	lower levels	1727:1738	lower levels of glycan occupancy	1727:1758	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	10	theme	occupancy	1750:1758	arg1	occupancy					1750:1758	glycan occupancy	1743:1758	glycan occupancy	1743:1758	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	11	11	theme	viral	1986:1990	arg1	glycoproteins					1992:2004	heterologous viral glycoproteins	1973:2004	heterologous viral glycoproteins	1973:2004	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	7	12	from	virus	1287:1291	arg1	glycoproteins					1252:1264	model glycoproteins	1246:1264	model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells	1246:1336	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	7	12	from	virus	1287:1291	arg1	cells					1332:1336	mammalian cells	1322:1336	mammalian cells	1322:1336	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	7	12	from	virus	1287:1291	arg1	benthamiana					1306:1316	Nicotiana benthamiana	1296:1316	Nicotiana benthamiana	1296:1316	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	8	13	theme	site-specific	1376:1388	arg1	glycosylation					1390:1402	their site-specific glycosylation	1370:1402	their site-specific glycosylation	1370:1402	The proteins were purified and their site-specific glycosylation was determined by mass-spectrometry.					
34367227	1	14	theme	scale	141:145	arg1	manufacturing					165:177	large scale biopharmaceutical manufacturing	135:177	large scale biopharmaceutical manufacturing capacity	135:186	There is an urgent need to establish large scale biopharmaceutical manufacturing capacity in Africa where the infrastructure for biologics production is severely limited.					
34367227	6	15	theme	relevant	1086:1093	arg1	vaccines					1095:1102	immunologically relevant vaccines	1070:1102	immunologically relevant vaccines	1070:1102	In many cases it may be necessary to reproduce features of their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens.					
34367227	11	16	theme	glycoproteins	1894:1906	arg1	glycosylation					1871:1883	the site-specific glycosylation	1853:1883	the site-specific glycosylation of viral glycoproteins produced in N. benthamiana	1853:1933	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	7	17	theme	Nicotiana	1296:1304	arg1	benthamiana					1306:1316	Nicotiana benthamiana	1296:1316	Nicotiana benthamiana	1296:1316	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	1	18	theme	manufacturing	165:177	arg1	capacity					179:186	large scale biopharmaceutical manufacturing capacity	135:186	large scale biopharmaceutical manufacturing capacity	135:186	There is an urgent need to establish large scale biopharmaceutical manufacturing capacity in Africa where the infrastructure for biologics production is severely limited.					
34367227	5	19	theme	furin	825:829	arg1	proteases					831:839	furin proteases	825:839	furin proteases	825:839	Whilst the co-expression of human chaperones and furin proteases has shown promise, it is presently unclear how plant-specific differences in glycosylation impact the production of these proteins.					
34367227	5	20	theme	proteins	963:970	arg1	production					943:952	the production	939:952	the production of these proteins	939:970	Whilst the co-expression of human chaperones and furin proteases has shown promise, it is presently unclear how plant-specific differences in glycosylation impact the production of these proteins.					
34367227	6	21	theme	glycosylation	1045:1057	arg1	features					1020:1027	features	1020:1027	features of their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens	1020:1191	In many cases it may be necessary to reproduce features of their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens.					
34367227	10	22	theme	glycosylation	1596:1608	arg1	profiles					1610:1617	The glycosylation profiles	1592:1617	The glycosylation profiles of the plant-produced glycoproteins	1592:1653	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	22	theme	glycosylation	1596:1608	arg1	distinct					1660:1667	distinct	1660:1667	distinct	1660:1667	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	3	23	theme	protein	505:511	arg1	pathway					523:529	the plant protein secretory pathway	495:529	the plant protein secretory pathway	495:529	However, there are several differences along the plant protein secretory pathway compared to mammalian systems, which constrain the production of complex pharmaceuticals.					
34367227	9	24	gly	glycoproteins	1446:1458	arg1	glycoproteins					1446:1458	Both glycoproteins	1441:1458	Both glycoproteins	1441:1458	Both glycoproteins yielded increased amounts of protein aggregates when produced in plants compared to the equivalent mammalian cell-derived proteins.					
34367227	2	25	theme	large-scale	426:436	arg1	production					438:447	rapid large-scale production	420:447	rapid large-scale production	420:447	Molecular farming, whereby pharmaceuticals are produced in plants, offers a cheaper alternative to mainstream expression platforms, and is amenable to rapid large-scale production.					
34367227	0	26	theme	Glycoproteins	49:61	arg1	Glycosylation					14:26	Site-Specific Glycosylation	0:26	Site-Specific Glycosylation of Recombinant Viral Glycoproteins	0:61	Site-Specific Glycosylation of Recombinant Viral Glycoproteins Produced in Nicotiana benthamiana.					
34367227	0	27	theme	Site-Specific	0:12	arg1	Glycosylation					14:26	Site-Specific Glycosylation	0:26	Site-Specific Glycosylation of Recombinant Viral Glycoproteins	0:61	Site-Specific Glycosylation of Recombinant Viral Glycoproteins Produced in Nicotiana benthamiana.					
34367227	10	28	theme	plant-produced	1626:1639	arg1	glycoproteins					1641:1653	the plant-produced glycoproteins	1622:1653	the plant-produced glycoproteins	1622:1653	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	3	29	theme	plant	499:503	arg1	pathway					523:529	the plant protein secretory pathway	495:529	the plant protein secretory pathway	495:529	However, there are several differences along the plant protein secretory pathway compared to mammalian systems, which constrain the production of complex pharmaceuticals.					
34367227	0	30	theme	Nicotiana	75:83	arg1	benthamiana					85:95	Nicotiana benthamiana	75:95	Nicotiana benthamiana	75:95	Site-Specific Glycosylation of Recombinant Viral Glycoproteins Produced in Nicotiana benthamiana.					
34367227	10	31	theme	paucimannosidic	1806:1820	arg1	structures					1822:1831	paucimannosidic structures	1806:1831	paucimannosidic structures	1806:1831	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	0	32	theme	Viral	43:47	arg1	Glycoproteins					49:61	Recombinant Viral Glycoproteins	31:61	Recombinant Viral Glycoproteins	31:61	Site-Specific Glycosylation of Recombinant Viral Glycoproteins Produced in Nicotiana benthamiana.					
34367227	0	33	gly	Glycosylation	14:26	arg1	Glycoproteins					49:61	Recombinant Viral Glycoproteins	31:61	Recombinant Viral Glycoproteins	31:61	Site-Specific Glycosylation of Recombinant Viral Glycoproteins Produced in Nicotiana benthamiana.					
34367227	5	34	theme	chaperones	810:819	arg1	co-expression					787:799	the co-expression	783:799	the co-expression of human chaperones and furin proteases	783:839	Whilst the co-expression of human chaperones and furin proteases has shown promise, it is presently unclear how plant-specific differences in glycosylation impact the production of these proteins.					
34367227	11	35	gly	glycoproteins	1992:2004	arg1	glycoproteins					1992:2004	heterologous viral glycoproteins	1973:2004	heterologous viral glycoproteins	1973:2004	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	2	36	theme	expression	379:388	arg1	platforms					390:398	mainstream expression platforms	368:398	mainstream expression platforms	368:398	Molecular farming, whereby pharmaceuticals are produced in plants, offers a cheaper alternative to mainstream expression platforms, and is amenable to rapid large-scale production.					
34367227	7	37	theme	previous	1206:1213	arg1	work					1215:1218	previous work	1206:1218	previous work	1206:1218	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	6	38	from	necessary	997:1005	arg1	cases					981:985	many cases	976:985	many cases	976:985	In many cases it may be necessary to reproduce features of their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens.					
34367227	11	39	theme	viral	1888:1892	arg1	glycoproteins					1894:1906	viral glycoproteins	1888:1906	viral glycoproteins produced in N. benthamiana	1888:1933	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	3	40	dep	are	465:467	arg1	constrain					568:576	constrain	568:576	constrain the production of complex pharmaceuticals	568:618	However, there are several differences along the plant protein secretory pathway compared to mammalian systems, which constrain the production of complex pharmaceuticals.					
34367227	3	41	theme	secretory	513:521	arg1	pathway					523:529	the plant protein secretory pathway	495:529	the plant protein secretory pathway	495:529	However, there are several differences along the plant protein secretory pathway compared to mammalian systems, which constrain the production of complex pharmaceuticals.					
34367227	9	42	theme	protein	1489:1495	arg1	aggregates					1497:1506	protein aggregates	1489:1506	protein aggregates	1489:1506	Both glycoproteins yielded increased amounts of protein aggregates when produced in plants compared to the equivalent mammalian cell-derived proteins.					
34367227	10	43	theme	produced	1693:1700	arg1	proteins					1702:1709	the mammalian cell produced proteins	1674:1709	the mammalian cell produced proteins	1674:1709	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	11	44	theme	glycosylation	1871:1883	arg1	step					1951:1954	an important step	1938:1954	an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation	1938:2054	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	11	44	theme	glycosylation	1871:1883	arg1	elucidation					1838:1848	The elucidation	1834:1848	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana	1834:1933	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	6	45	from	cases	981:985	arg1	necessary					997:1005	necessary	997:1005	necessary	997:1005	In many cases it may be necessary to reproduce features of their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens.					
34367227	3	46	theme	mammalian	543:551	arg1	systems					553:559	mammalian systems	543:559	mammalian systems	543:559	However, there are several differences along the plant protein secretory pathway compared to mammalian systems, which constrain the production of complex pharmaceuticals.					
34367227	4	47	theme	envelope	627:634	arg1	glycoproteins					636:648	Viral envelope glycoproteins	621:648	Viral envelope glycoproteins	621:648	Viral envelope glycoproteins are important targets for immunization, yet in some cases they accumulate poorly in plants and may not be properly processed.					
34367227	4	47	theme	envelope	627:634	arg1	targets					664:670	important targets	654:670	important targets for immunization	654:687	Viral envelope glycoproteins are important targets for immunization, yet in some cases they accumulate poorly in plants and may not be properly processed.					
34367227	11	48	theme	human-like	2031:2040	arg1	glycosylation					2042:2054	authentic human-like glycosylation	2021:2054	authentic human-like glycosylation	2021:2054	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	10	49	gly	glycoproteins	1641:1653	arg1	glycoproteins					1641:1653	the plant-produced glycoproteins	1622:1653	the plant-produced glycoproteins	1622:1653	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	50	theme	glycan	1743:1748	arg1	occupancy					1750:1758	glycan occupancy	1743:1758	glycan occupancy	1743:1758	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	2	51	theme	cheaper	345:351	arg1	alternative					353:363	a cheaper alternative	343:363	a cheaper alternative to mainstream expression platforms	343:398	Molecular farming, whereby pharmaceuticals are produced in plants, offers a cheaper alternative to mainstream expression platforms, and is amenable to rapid large-scale production.					
34367227	9	52	theme	mammalian	1559:1567	arg1	proteins					1582:1589	the equivalent mammalian cell-derived proteins	1544:1589	the equivalent mammalian cell-derived proteins	1544:1589	Both glycoproteins yielded increased amounts of protein aggregates when produced in plants compared to the equivalent mammalian cell-derived proteins.					
34367227	11	53	theme	heterologous	1973:1984	arg1	glycoproteins					1992:2004	heterologous viral glycoproteins	1973:2004	heterologous viral glycoproteins	1973:2004	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	3	54	theme	complex	596:602	arg1	pharmaceuticals					604:618	complex pharmaceuticals	596:618	complex pharmaceuticals	596:618	However, there are several differences along the plant protein secretory pathway compared to mammalian systems, which constrain the production of complex pharmaceuticals.					
34367227	10	55	theme	complex	1769:1775	arg1	glycans					1777:1783	reduced complex glycans	1761:1783	reduced complex glycans	1761:1783	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	2	56	theme	Molecular	269:277	arg1	farming					279:285	Molecular farming	269:285	Molecular farming	269:285	Molecular farming, whereby pharmaceuticals are produced in plants, offers a cheaper alternative to mainstream expression platforms, and is amenable to rapid large-scale production.					
34367227	5	57	theme	plant-specific	888:901	arg1	differences					903:913	plant-specific differences	888:913	plant-specific differences in glycosylation	888:930	Whilst the co-expression of human chaperones and furin proteases has shown promise, it is presently unclear how plant-specific differences in glycosylation impact the production of these proteins.					
34367227	7	58	from	HIV	1271:1273	arg1	glycoproteins					1252:1264	model glycoproteins	1246:1264	model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells	1246:1336	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	7	58	from	HIV	1271:1273	arg1	cells					1332:1336	mammalian cells	1322:1336	mammalian cells	1322:1336	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	7	58	from	HIV	1271:1273	arg1	benthamiana					1306:1316	Nicotiana benthamiana	1296:1316	Nicotiana benthamiana	1296:1316	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	5	59	from	differences	903:913	arg1	glycosylation					918:930	glycosylation	918:930	glycosylation	918:930	Whilst the co-expression of human chaperones and furin proteases has shown promise, it is presently unclear how plant-specific differences in glycosylation impact the production of these proteins.					
34367227	11	60	gly	glycosylation	2042:2054	arg1	plants					2009:2014	plants	2009:2014	plants with authentic human-like glycosylation	2009:2054	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	1	61	theme	large	135:139	arg1	manufacturing					165:177	large scale biopharmaceutical manufacturing	135:177	large scale biopharmaceutical manufacturing capacity	135:186	There is an urgent need to establish large scale biopharmaceutical manufacturing capacity in Africa where the infrastructure for biologics production is severely limited.					
34367227	11	62	theme	N.	1920:1921	arg1	benthamiana					1923:1933	N. benthamiana	1920:1933	N. benthamiana	1920:1933	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	5	63	theme	human	804:808	arg1	chaperones					810:819	human chaperones	804:819	human chaperones	804:819	Whilst the co-expression of human chaperones and furin proteases has shown promise, it is presently unclear how plant-specific differences in glycosylation impact the production of these proteins.					
34367227	1	64	theme	biopharmaceutical	147:163	arg1	manufacturing					165:177	large scale biopharmaceutical manufacturing	135:177	large scale biopharmaceutical manufacturing capacity	135:186	There is an urgent need to establish large scale biopharmaceutical manufacturing capacity in Africa where the infrastructure for biologics production is severely limited.					
34367227	7	65	theme	Marburg	1279:1285	arg1	virus					1287:1291	Marburg virus	1279:1291	Marburg virus	1279:1291	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	11	66	theme	important	1941:1949	arg1	step					1951:1954	an important step	1938:1954	an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation	1938:2054	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	11	66	theme	important	1941:1949	arg1	elucidation					1838:1848	The elucidation	1834:1848	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana	1834:1933	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	6	67	theme	native	1038:1043	arg1	glycosylation					1045:1057	their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens	1032:1191	their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens	1032:1191	In many cases it may be necessary to reproduce features of their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens.					
34367227	11	68	with	plants	2009:2014	arg1	glycosylation					2042:2054	authentic human-like glycosylation	2021:2054	authentic human-like glycosylation	2021:2054	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	7	69	gly	glycoproteins	1252:1264	arg1	glycoproteins					1252:1264	model glycoproteins	1246:1264	model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells	1246:1336	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	10	70	theme	large	1789:1793	arg1	amounts					1795:1801	large amounts	1789:1801	large amounts of paucimannosidic structures	1789:1831	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	70	theme	large	1789:1793	arg1	structures					1822:1831	paucimannosidic structures	1806:1831	paucimannosidic structures	1806:1831	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	70	theme	large	1789:1793	arg1	occupancy					1750:1758	glycan occupancy	1743:1758	glycan occupancy	1743:1758	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	4	71	gly	glycoproteins	636:648	arg1	glycoproteins					636:648	Viral envelope glycoproteins	621:648	Viral envelope glycoproteins	621:648	Viral envelope glycoproteins are important targets for immunization, yet in some cases they accumulate poorly in plants and may not be properly processed.					
34367227	4	71	gly	glycoproteins	636:648	arg1	targets					664:670	important targets	654:670	important targets for immunization	654:687	Viral envelope glycoproteins are important targets for immunization, yet in some cases they accumulate poorly in plants and may not be properly processed.					
34367227	0	72	theme	Recombinant	31:41	arg1	Glycoproteins					49:61	Recombinant Viral Glycoproteins	31:61	Recombinant Viral Glycoproteins	31:61	Site-Specific Glycosylation of Recombinant Viral Glycoproteins Produced in Nicotiana benthamiana.					
34367227	1	73	theme	urgent	110:115	arg1	need					117:120	an urgent need	107:120	an urgent need to establish large scale biopharmaceutical manufacturing capacity in Africa where the infrastructure for biologics production is severely limited	107:266	There is an urgent need to establish large scale biopharmaceutical manufacturing capacity in Africa where the infrastructure for biologics production is severely limited.					
34367227	4	74	theme	Viral	621:625	arg1	glycoproteins					636:648	Viral envelope glycoproteins	621:648	Viral envelope glycoproteins	621:648	Viral envelope glycoproteins are important targets for immunization, yet in some cases they accumulate poorly in plants and may not be properly processed.					
34367227	4	74	theme	Viral	621:625	arg1	targets					664:670	important targets	654:670	important targets for immunization	654:687	Viral envelope glycoproteins are important targets for immunization, yet in some cases they accumulate poorly in plants and may not be properly processed.					
34367227	11	75	gly	glycoproteins	1894:1906	arg1	glycoproteins					1894:1906	viral glycoproteins	1888:1906	viral glycoproteins produced in N. benthamiana	1888:1933	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	10	76	theme	structures	1822:1831	arg1	glycans					1777:1783	reduced complex glycans	1761:1783	reduced complex glycans	1761:1783	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	76	theme	structures	1822:1831	arg1	amounts					1795:1801	large amounts	1789:1801	large amounts of paucimannosidic structures	1789:1831	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	76	theme	structures	1822:1831	arg1	structures					1822:1831	paucimannosidic structures	1806:1831	paucimannosidic structures	1806:1831	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	76	theme	structures	1822:1831	arg1	levels					1733:1738	lower levels	1727:1738	lower levels of glycan occupancy	1727:1758	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	76	theme	structures	1822:1831	arg1	occupancy					1750:1758	glycan occupancy	1743:1758	glycan occupancy	1743:1758	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	5	77	theme	proteases	831:839	arg1	co-expression					787:799	the co-expression	783:799	the co-expression of human chaperones and furin proteases	783:839	Whilst the co-expression of human chaperones and furin proteases has shown promise, it is presently unclear how plant-specific differences in glycosylation impact the production of these proteins.					
34367227	3	78	theme	several	469:475	arg1	differences					477:487	several differences	469:487	several differences along the plant protein secretory pathway	469:529	However, there are several differences along the plant protein secretory pathway compared to mammalian systems, which constrain the production of complex pharmaceuticals.					
34367227	9	79	theme	increased	1468:1476	arg1	amounts					1478:1484	increased amounts	1468:1484	increased amounts of protein aggregates	1468:1506	Both glycoproteins yielded increased amounts of protein aggregates when produced in plants compared to the equivalent mammalian cell-derived proteins.					
34367227	9	79	theme	increased	1468:1476	arg1	aggregates					1497:1506	protein aggregates	1489:1506	protein aggregates	1489:1506	Both glycoproteins yielded increased amounts of protein aggregates when produced in plants compared to the equivalent mammalian cell-derived proteins.					
34367227	10	80	theme	mammalian	1678:1686	arg1	proteins					1702:1709	the mammalian cell produced proteins	1674:1709	the mammalian cell produced proteins	1674:1709	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	9	81	link	cell-derived	1569:1580	arg1	proteins					1582:1589	the equivalent mammalian cell-derived proteins	1544:1589	the equivalent mammalian cell-derived proteins	1544:1589	Both glycoproteins yielded increased amounts of protein aggregates when produced in plants compared to the equivalent mammalian cell-derived proteins.					
34367227	2	82	theme	mainstream	368:377	arg1	platforms					390:398	mainstream expression platforms	368:398	mainstream expression platforms	368:398	Molecular farming, whereby pharmaceuticals are produced in plants, offers a cheaper alternative to mainstream expression platforms, and is amenable to rapid large-scale production.					
34367227	10	83	from	proteins	1702:1709	arg1	profiles					1610:1617	The glycosylation profiles	1592:1617	The glycosylation profiles of the plant-produced glycoproteins	1592:1653	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	83	from	proteins	1702:1709	arg1	distinct					1660:1667	distinct	1660:1667	distinct	1660:1667	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	6	84	theme	antigens	1184:1191	arg1	immunogenicity					1160:1173	immunogenicity	1160:1173	immunogenicity	1160:1173	In many cases it may be necessary to reproduce features of their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens.					
34367227	6	84	theme	antigens	1184:1191	arg1	folding					1148:1154	folding	1148:1154	folding	1148:1154	In many cases it may be necessary to reproduce features of their native glycosylation to produce immunologically relevant vaccines, given that glycosylation is central to the folding and immunogenicity of these antigens.					
34367227	10	85	gly	glycosylation	1596:1608	arg1	glycoproteins					1641:1653	the plant-produced glycoproteins	1622:1653	the plant-produced glycoproteins	1622:1653	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	86	theme	glycoproteins	1641:1653	arg1	profiles					1610:1617	The glycosylation profiles	1592:1617	The glycosylation profiles of the plant-produced glycoproteins	1592:1653	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	10	86	theme	glycoproteins	1641:1653	arg1	distinct					1660:1667	distinct	1660:1667	distinct	1660:1667	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
34367227	11	87	theme	site-specific	1857:1869	arg1	glycosylation					1871:1883	the site-specific glycosylation	1853:1883	the site-specific glycosylation of viral glycoproteins produced in N. benthamiana	1853:1933	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	11	88	theme	authentic	2021:2029	arg1	glycosylation					2042:2054	authentic human-like glycosylation	2021:2054	authentic human-like glycosylation	2021:2054	The elucidation of the site-specific glycosylation of viral glycoproteins produced in N. benthamiana is an important step toward producing heterologous viral glycoproteins in plants with authentic human-like glycosylation.					
34367227	9	89	theme	aggregates	1497:1506	arg1	amounts					1478:1484	increased amounts	1468:1484	increased amounts of protein aggregates	1468:1506	Both glycoproteins yielded increased amounts of protein aggregates when produced in plants compared to the equivalent mammalian cell-derived proteins.					
34367227	9	89	theme	aggregates	1497:1506	arg1	aggregates					1497:1506	protein aggregates	1489:1506	protein aggregates	1489:1506	Both glycoproteins yielded increased amounts of protein aggregates when produced in plants compared to the equivalent mammalian cell-derived proteins.					
34367227	7	90	theme	mammalian	1322:1330	arg1	cells					1332:1336	mammalian cells	1322:1336	mammalian cells	1322:1336	Building on previous work, we transiently expressed model glycoproteins from HIV and Marburg virus in Nicotiana benthamiana and mammalian cells.					
34367227	2	91	theme	rapid	420:424	arg1	production					438:447	rapid large-scale production	420:447	rapid large-scale production	420:447	Molecular farming, whereby pharmaceuticals are produced in plants, offers a cheaper alternative to mainstream expression platforms, and is amenable to rapid large-scale production.					
34367227	10	92	theme	cell	1688:1691	arg1	proteins					1702:1709	the mammalian cell produced proteins	1674:1709	the mammalian cell produced proteins	1674:1709	The glycosylation profiles of the plant-produced glycoproteins were distinct from the mammalian cell produced proteins: they displayed lower levels of glycan occupancy, reduced complex glycans and large amounts of paucimannosidic structures.					
31922547	2	0	gly	glycoproteins	304:316	arg1	glycoproteins					304:316	therapeutic glycoproteins	292:316	therapeutic glycoproteins with human-compatible N-glycosylation	292:354	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	0	1	from	role	18:21	arg1	processing					43:52	antibody processing	34:52	antibody processing in glycoengineered Saccharomyces cerevisiae	34:96	Investigating the role of ERAD on antibody processing in glycoengineered Saccharomyces cerevisiae.					
31922547	4	2	theme	ALG3	925:928	arg1	ERAD					930:933	ALG3 ERAD	925:933	ALG3 ERAD	925:933	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	1	3	theme	quality	168:174	arg1	ERQC					185:188	ERQC	185:188	ERQC	185:188	N-glycosylation plays an important role in the endoplasmic reticulum quality control (ERQC).					
31922547	1	3	theme	quality	168:174	arg1	control					176:182	the endoplasmic reticulum quality control	142:182	the endoplasmic reticulum quality control (ERQC)	142:189	N-glycosylation plays an important role in the endoplasmic reticulum quality control (ERQC).					
31922547	4	4	from	presence	913:920	arg1	dependent					948:956	dependent	948:956	dependent	948:956	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	0	5	theme	Saccharomyces	73:85	arg1	cerevisiae					87:96	glycoengineered Saccharomyces cerevisiae	57:96	glycoengineered Saccharomyces cerevisiae	57:96	Investigating the role of ERAD on antibody processing in glycoengineered Saccharomyces cerevisiae.					
31922547	4	6	from	dependent	948:956	arg1	presence					913:920	the presence	909:920	the presence of ALG3 ERAD	909:933	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	3	7	theme	reticulum-associated	699:718	arg1	degradation					720:730	endoplasmic reticulum-associated degradation	687:730	endoplasmic reticulum-associated degradation (ERAD)	687:737	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	3	7	theme	reticulum-associated	699:718	arg1	ERAD					733:736	ERAD	733:736	ERAD	733:736	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	2	8	with	glycoproteins	304:316	arg1	N-glycosylation					340:354	human-compatible N-glycosylation	323:354	human-compatible N-glycosylation	323:354	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	2	9	theme	therapeutic	292:302	arg1	glycoproteins					304:316	therapeutic glycoproteins	292:316	therapeutic glycoproteins with human-compatible N-glycosylation	292:354	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	3	10	theme	Δalg11	601:606	arg1	strain					633:638	glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	579:638	glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	579:638	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	4	11	theme	Δalg11	809:814	arg1	strain					816:821	Δalg3 Δalg11 strain	803:821	Δalg3 Δalg11 strain	803:821	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	0	12	from	processing	43:52	arg1	cerevisiae					87:96	glycoengineered Saccharomyces cerevisiae	57:96	glycoengineered Saccharomyces cerevisiae	57:96	Investigating the role of ERAD on antibody processing in glycoengineered Saccharomyces cerevisiae.					
31922547	4	13	theme	ERAD	827:830	arg1	substrates					832:841	ERAD substrates	827:841	ERAD substrates	827:841	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	13	theme	ERAD	827:830	arg1	light					758:762	Intracellular IgG light and heavy chain molecules	740:788	light	758:762	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	13	theme	ERAD	827:830	arg1	molecules					780:788	Intracellular IgG light and heavy chain molecules	740:788	molecules	780:788	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	3	14	theme	glycoengineered	579:593	arg1	strain					633:638	glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	579:638	glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	579:638	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	1	15	theme	important	124:132	arg1	role					134:137	an important role	121:137	an important role	121:137	N-glycosylation plays an important role in the endoplasmic reticulum quality control (ERQC).					
31922547	6	16	theme	other	1280:1284	arg1	mechanisms					1286:1295	other mechanisms	1280:1295	other mechanisms than the Htm1p-Yos9p-dependent route	1280:1332	Our results show ERAD targeting of a heterologous glycoprotein in yeast, and suggest that proteins in the ER can be targeted to ERAD via other mechanisms than the Htm1p-Yos9p-dependent route when the LLO biosynthesis is altered.					
31922547	3	17	from	processing	558:567	arg1	strain					633:638	glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	579:638	glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	579:638	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	5	18	theme	ER	1024:1025	arg1	forms					1042:1046	ER and post-Golgi forms	1024:1046	forms	1042:1046	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	2	19	theme	oligosaccharide	434:448	arg1	synthesis					404:412	the synthesis	400:412	the synthesis of the lipid-linked oligosaccharide (LLO)	400:454	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	6	20	theme	ERAD	1160:1163	arg1	targeting					1165:1173	ERAD targeting	1160:1173	ERAD targeting of a heterologous glycoprotein in yeast	1160:1213	Our results show ERAD targeting of a heterologous glycoprotein in yeast, and suggest that proteins in the ER can be targeted to ERAD via other mechanisms than the Htm1p-Yos9p-dependent route when the LLO biosynthesis is altered.					
31922547	3	21	theme	IgG	572:574	arg1	processing					558:567	intracellular processing	544:567	intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	544:638	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	2	22	theme	lipid-linked	421:432	arg1	LLO					451:453	LLO	451:453	LLO	451:453	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	2	22	theme	lipid-linked	421:432	arg1	oligosaccharide					434:448	the lipid-linked oligosaccharide	417:448	the lipid-linked oligosaccharide (LLO)	417:454	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	3	23	dep	Saccharomyces	608:620	arg1	cerevisiae					622:631	cerevisiae	622:631	cerevisiae	622:631	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	6	24	theme	Htm1p-Yos9p-dependent	1306:1326	arg1	route					1328:1332	the Htm1p-Yos9p-dependent route	1302:1332	the Htm1p-Yos9p-dependent route	1302:1332	Our results show ERAD targeting of a heterologous glycoprotein in yeast, and suggest that proteins in the ER can be targeted to ERAD via other mechanisms than the Htm1p-Yos9p-dependent route when the LLO biosynthesis is altered.					
31922547	3	25	theme	Saccharomyces	608:620	arg1	strain					633:638	glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	579:638	glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	579:638	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	4	26	theme	Intracellular	740:752	arg1	substrates					832:841	ERAD substrates	827:841	ERAD substrates	827:841	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	26	theme	Intracellular	740:752	arg1	light					758:762	Intracellular IgG light and heavy chain molecules	740:788	light	758:762	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	26	theme	Intracellular	740:752	arg1	molecules					780:788	Intracellular IgG light and heavy chain molecules	740:788	molecules	780:788	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	2	27	theme	human-compatible	323:338	arg1	N-glycosylation					340:354	human-compatible N-glycosylation	323:354	human-compatible N-glycosylation	323:354	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	5	28	theme	IgG	1128:1130	arg1	secretion					1132:1140	IgG secretion	1128:1140	IgG secretion	1128:1140	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	3	29	theme	Δalg3	595:599	arg1	strain					633:638	glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	579:638	glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	579:638	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	4	30	theme	Δalg3	803:807	arg1	strain					816:821	Δalg3 Δalg11 strain	803:821	Δalg3 Δalg11 strain	803:821	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	2	31	theme	glycoproteins	304:316	arg1	production					278:287	the production	274:287	the production of therapeutic glycoproteins with human-compatible N-glycosylation	274:354	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	3	32	theme	intracellular	544:556	arg1	processing					558:567	intracellular processing	544:567	intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain	544:638	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	4	33	dep	substrates	832:841	arg1	whereas					898:904	whereas	898:904	whereas	898:904	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	3	34	theme	LLO	480:482	arg1	engineering					484:494	LLO engineering	480:494	LLO engineering	480:494	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	0	35	theme	ERAD	26:29	arg1	role					18:21	the role	14:21	the role of ERAD on antibody processing in glycoengineered Saccharomyces cerevisiae	14:96	Investigating the role of ERAD on antibody processing in glycoengineered Saccharomyces cerevisiae.					
31922547	3	36	theme	endoplasmic	687:697	arg1	degradation					720:730	endoplasmic reticulum-associated degradation	687:730	endoplasmic reticulum-associated degradation (ERAD)	687:737	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	3	36	theme	endoplasmic	687:697	arg1	ERAD					733:736	ERAD	733:736	ERAD	733:736	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	5	37	theme	increased	1059:1067	arg1	glycosylation					1069:1081	increased glycosylation	1059:1081	increased glycosylation of matα secretion signal	1059:1106	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	3	38	theme	engineering	484:494	arg1	effects					469:475	the effects	465:475	the effects of LLO engineering on ERQC	465:502	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	3	38	theme	engineering	484:494	arg1	unknown					518:524	unknown	518:524	unknown	518:524	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	6	39	theme	LLO	1343:1345	arg1	biosynthesis					1347:1358	the LLO biosynthesis	1339:1358	the LLO biosynthesis	1339:1358	Our results show ERAD targeting of a heterologous glycoprotein in yeast, and suggest that proteins in the ER can be targeted to ERAD via other mechanisms than the Htm1p-Yos9p-dependent route when the LLO biosynthesis is altered.					
31922547	2	40	link	lipid-linked	421:432	arg1	LLO					451:453	LLO	451:453	LLO	451:453	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	2	40	link	lipid-linked	421:432	arg1	oligosaccharide					434:448	the lipid-linked oligosaccharide	417:448	the lipid-linked oligosaccharide (LLO)	417:454	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	5	41	theme	glycosylation	1069:1081	arg1	forms					1042:1046	ER and post-Golgi forms	1024:1046	forms	1042:1046	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	2	42	theme	glycoengineering	366:381	arg1	approaches					383:392	some glycoengineering approaches	361:392	some glycoengineering approaches	361:392	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	0	43	theme	antibody	34:41	arg1	processing					43:52	antibody processing	34:52	antibody processing in glycoengineered Saccharomyces cerevisiae	34:96	Investigating the role of ERAD on antibody processing in glycoengineered Saccharomyces cerevisiae.					
31922547	5	44	theme	matα	1086:1089	arg1	signal					1101:1106	matα secretion signal	1086:1106	matα secretion signal	1086:1106	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	4	45	theme	chain	774:778	arg1	molecules					780:788	Intracellular IgG light and heavy chain molecules	740:788	molecules	780:788	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	45	theme	chain	774:778	arg1	light					758:762	Intracellular IgG light and heavy chain molecules	740:788	light	758:762	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	45	theme	chain	774:778	arg1	substrates					832:841	ERAD substrates	827:841	ERAD substrates	827:841	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	5	46	theme	IgG	1051:1053	arg1	forms					1042:1046	ER and post-Golgi forms	1024:1046	forms	1042:1046	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	5	47	theme	secretion	1091:1099	arg1	signal					1101:1106	matα secretion signal	1086:1106	matα secretion signal	1086:1106	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	5	48	theme	post-Golgi	1031:1040	arg1	forms					1042:1046	ER and post-Golgi forms	1024:1046	forms	1042:1046	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	6	49	from	targeting	1165:1173	arg1	yeast					1209:1213	yeast	1209:1213	yeast	1209:1213	Our results show ERAD targeting of a heterologous glycoprotein in yeast, and suggest that proteins in the ER can be targeted to ERAD via other mechanisms than the Htm1p-Yos9p-dependent route when the LLO biosynthesis is altered.					
31922547	4	50	theme	heavy	768:772	arg1	molecules					780:788	Intracellular IgG light and heavy chain molecules	740:788	molecules	780:788	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	50	theme	heavy	768:772	arg1	light					758:762	Intracellular IgG light and heavy chain molecules	740:788	light	758:762	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	50	theme	heavy	768:772	arg1	substrates					832:841	ERAD substrates	827:841	ERAD substrates	827:841	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	6	51	theme	heterologous	1180:1191	arg1	glycoprotein					1193:1204	a heterologous glycoprotein	1178:1204	a heterologous glycoprotein	1178:1204	Our results show ERAD targeting of a heterologous glycoprotein in yeast, and suggest that proteins in the ER can be targeted to ERAD via other mechanisms than the Htm1p-Yos9p-dependent route when the LLO biosynthesis is altered.					
31922547	6	52	gly	glycoprotein	1193:1204	arg1	glycoprotein					1193:1204	a heterologous glycoprotein	1178:1204	a heterologous glycoprotein	1178:1204	Our results show ERAD targeting of a heterologous glycoprotein in yeast, and suggest that proteins in the ER can be targeted to ERAD via other mechanisms than the Htm1p-Yos9p-dependent route when the LLO biosynthesis is altered.					
31922547	2	53	theme	biosynthesis	201:212	arg1	pathways					214:221	N-glycan biosynthesis pathways	192:221	N-glycan biosynthesis pathways	192:221	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	5	54	theme	ERAD	1007:1010	arg1	Blocking					995:1002	Blocking	995:1002	Blocking of ERAD	995:1010	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	4	55	theme	IgG	754:756	arg1	substrates					832:841	ERAD substrates	827:841	ERAD substrates	827:841	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	55	theme	IgG	754:756	arg1	light					758:762	Intracellular IgG light and heavy chain molecules	740:788	light	758:762	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	55	theme	IgG	754:756	arg1	molecules					780:788	Intracellular IgG light and heavy chain molecules	740:788	molecules	780:788	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	4	56	theme	ERAD	930:933	arg1	presence					913:920	the presence	909:920	the presence of ALG3 ERAD	909:933	Intracellular IgG light and heavy chain molecules expressed in Δalg3 Δalg11 strain are ERAD substrates and targeted to ERAD independently of Yos9p and Htm1p, whereas in the presence of ALG3 ERAD targeting is dependent on Yos9p but does not require Htm1p.					
31922547	5	57	theme	signal	1101:1106	arg1	IgG					1051:1053	IgG	1051:1053	IgG	1051:1053	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	5	57	theme	signal	1101:1106	arg1	glycosylation					1069:1081	increased glycosylation	1059:1081	increased glycosylation of matα secretion signal	1059:1106	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	0	58	theme	glycoengineered	57:71	arg1	cerevisiae					87:96	glycoengineered Saccharomyces cerevisiae	57:96	glycoengineered Saccharomyces cerevisiae	57:96	Investigating the role of ERAD on antibody processing in glycoengineered Saccharomyces cerevisiae.					
31922547	2	59	theme	N-glycan	192:199	arg1	pathways					214:221	N-glycan biosynthesis pathways	192:221	N-glycan biosynthesis pathways	192:221	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	3	60	theme	altered	657:663	arg1	structures					669:678	altered LLO structures	657:678	altered LLO structures	657:678	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	5	61	gly	glycosylation	1069:1081	arg1	signal					1101:1106	matα secretion signal	1086:1106	matα secretion signal	1086:1106	Blocking of ERAD accumulated ER and post-Golgi forms of IgG and increased glycosylation of matα secretion signal but did not improve IgG secretion.					
31922547	1	62	theme	endoplasmic	146:156	arg1	reticulum					158:166	the endoplasmic reticulum	142:166	the endoplasmic reticulum quality control (ERQC)	142:189	N-glycosylation plays an important role in the endoplasmic reticulum quality control (ERQC).					
31922547	6	63	theme	glycoprotein	1193:1204	arg1	targeting					1165:1173	ERAD targeting	1160:1173	ERAD targeting of a heterologous glycoprotein in yeast	1160:1213	Our results show ERAD targeting of a heterologous glycoprotein in yeast, and suggest that proteins in the ER can be targeted to ERAD via other mechanisms than the Htm1p-Yos9p-dependent route when the LLO biosynthesis is altered.					
31922547	2	64	gly	N-glycosylation	340:354	arg1	glycoproteins					304:316	therapeutic glycoproteins	292:316	therapeutic glycoproteins with human-compatible N-glycosylation	292:354	N-glycan biosynthesis pathways have been engineered in yeasts and fungi to enable the production of therapeutic glycoproteins with human-compatible N-glycosylation, and some glycoengineering approaches alter the synthesis of the lipid-linked oligosaccharide (LLO).					
31922547	3	65	theme	LLO	665:667	arg1	structures					669:678	altered LLO structures	657:678	altered LLO structures	657:678	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	3	66	from	effects	469:475	arg1	ERQC					499:502	ERQC	499:502	ERQC	499:502	Because the effects of LLO engineering on ERQC are currently unknown, we characterized intracellular processing of IgG in glycoengineered Δalg3 Δalg11 Saccharomyces cerevisiae strain and analyzed how altered LLO structures affect endoplasmic reticulum-associated degradation (ERAD).					
31922547	1	67	theme	reticulum	158:166	arg1	ERQC					185:188	ERQC	185:188	ERQC	185:188	N-glycosylation plays an important role in the endoplasmic reticulum quality control (ERQC).					
31922547	1	67	theme	reticulum	158:166	arg1	control					176:182	the endoplasmic reticulum quality control	142:182	the endoplasmic reticulum quality control (ERQC)	142:189	N-glycosylation plays an important role in the endoplasmic reticulum quality control (ERQC).					
31922547	6	68	from	proteins	1233:1240	arg1	ER					1249:1250	the ER	1245:1250	the ER	1245:1250	Our results show ERAD targeting of a heterologous glycoprotein in yeast, and suggest that proteins in the ER can be targeted to ERAD via other mechanisms than the Htm1p-Yos9p-dependent route when the LLO biosynthesis is altered.					
35066229	4	0	theme	biomolecular	678:689	arg1	corona					691:696	the biomolecular corona	674:696	the biomolecular corona	674:696	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	2	1	theme	biological	358:367	arg1	medium					369:374	the biological medium	354:374	the biological medium	354:374	It is well accepted that the biomolecular corona dramatically affects the NP fate in the biological medium while the pristine surface is no longer available for binding.					
35066229	9	2	gly	sialylated	1590:1599	arg1	A2G2S2					1610:1615	A2G2S2	1610:1615	A2G2S2	1610:1615	However, under both plasma concentrations, the biantennary sialylated glycans (A2G2S2) are enriched.					
35066229	9	2	gly	sialylated	1590:1599	arg1	glycans					1601:1607	the biantennary sialylated glycans	1574:1607	the biantennary sialylated glycans (A2G2S2)	1574:1616	However, under both plasma concentrations, the biantennary sialylated glycans (A2G2S2) are enriched.					
35066229	8	3	theme	corona	1471:1476	arg1	dependent					1493:1501	dependent	1493:1501	dependent	1493:1501	FINDINGS Our findings highlighted that the protein corona composition is dependent on the exposing conditions.					
35066229	8	3	theme	corona	1471:1476	arg1	composition					1478:1488	the protein corona composition	1459:1488	the protein corona composition	1459:1488	FINDINGS Our findings highlighted that the protein corona composition is dependent on the exposing conditions.					
35066229	10	4	theme	known	1740:1744	arg1	affinity					1746:1753	known affinity	1740:1753	known affinity towards terminal sialic acids	1740:1783	DLS and FCS confirmed that the glycans are accessible for binding as the corona interacts with lectins with known affinity towards terminal sialic acids and the enzymatic removal of the glycans leads to a decrease in lectin affinity.					
35066229	4	5	from	interaction	745:755	arg1	corona					782:787	the corona	778:787	the corona	778:787	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	7	6	theme	binding	1207:1213	arg1	assay					1215:1219	a lectin binding assay	1198:1219	a lectin binding assay	1198:1219	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	11	7	from	corona	1938:1943	arg1	glycans					1911:1917	the glycans	1907:1917	the glycans	1907:1917	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	11	7	from	corona	1938:1943	arg1	present					1923:1929	present	1923:1929	present	1923:1929	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	10	8	theme	glycans	1818:1824	arg1	removal					1803:1809	the enzymatic removal	1789:1809	the enzymatic removal of the glycans	1789:1824	DLS and FCS confirmed that the glycans are accessible for binding as the corona interacts with lectins with known affinity towards terminal sialic acids and the enzymatic removal of the glycans leads to a decrease in lectin affinity.					
35066229	8	9	theme	protein	1463:1469	arg1	dependent					1493:1501	dependent	1493:1501	dependent	1493:1501	FINDINGS Our findings highlighted that the protein corona composition is dependent on the exposing conditions.					
35066229	8	9	theme	protein	1463:1469	arg1	composition					1478:1488	the protein corona composition	1459:1488	the protein corona composition	1459:1488	FINDINGS Our findings highlighted that the protein corona composition is dependent on the exposing conditions.					
35066229	11	10	theme	processes	2028:2036	arg1	modulation					2000:2009	the modulation	1996:2009	the modulation of NP biological processes	1996:2036	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	3	11	theme	NP	549:550	arg1	interaction					552:562	the NP interaction	545:562	the NP interaction with macrophages	545:579	Recent studies have shown that the glycans associated with the proteins forming the corona have a role in the NP interaction with macrophages, but the glycan identities remain unknown.					
35066229	5	12	theme	biomolecular	893:904	arg1	corona					906:911	the biomolecular corona	889:911	the biomolecular corona of citrate	889:922	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	10	13	theme	terminal	1763:1770	arg1	acids					1779:1783	terminal sialic acids	1763:1783	terminal sialic acids	1763:1783	DLS and FCS confirmed that the glycans are accessible for binding as the corona interacts with lectins with known affinity towards terminal sialic acids and the enzymatic removal of the glycans leads to a decrease in lectin affinity.					
35066229	5	14	theme	gold	935:938	arg1	NPs					940:942	gold NPs	935:942	gold NPs	935:942	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	11	15	located	present	1923:1929	arg1	corona					1938:1943	the corona	1934:1943	the corona	1934:1943	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	11	15	located	present	1923:1929	arg2	present					1923:1929	present	1923:1929	present	1923:1929	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	11	15	located	present	1923:1929	arg2	glycans					1911:1917	the glycans	1907:1917	the glycans	1907:1917	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	4	16	theme	glycans	730:736	arg1	role					716:719	the role	712:719	the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins	712:837	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	11	17	from	present	1923:1929	arg1	corona					1938:1943	the corona	1934:1943	the corona	1934:1943	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	10	18	from	decrease	1837:1844	arg1	affinity					1856:1863	lectin affinity	1849:1863	lectin affinity	1849:1863	DLS and FCS confirmed that the glycans are accessible for binding as the corona interacts with lectins with known affinity towards terminal sialic acids and the enzymatic removal of the glycans leads to a decrease in lectin affinity.					
35066229	7	19	theme	specific	1377:1384	arg1	glycans					1386:1392	specific glycans	1377:1392	specific glycans	1377:1392	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	4	20	theme	glycan	652:657	arg1	composition					659:669	the glycan composition	648:669	the glycan composition of the biomolecular corona	648:696	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	3	21	theme	Recent	439:444	arg1	studies					446:452	Recent studies	439:452	Recent studies	439:452	Recent studies have shown that the glycans associated with the proteins forming the corona have a role in the NP interaction with macrophages, but the glycan identities remain unknown.					
35066229	7	22	with	proteins	1340:1347	arg1	affinity					1360:1367	known affinity	1354:1367	known affinity towards specific glycans	1354:1392	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	5	23	theme	in	1053:1054	arg1	conditions					1061:1070	the in vitro and in vivo conditions	1036:1070	the in vitro and in vivo conditions	1036:1070	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	4	24	theme	binding	801:807	arg1	lectins					831:837	lectins	831:837	lectins	831:837	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	4	24	theme	binding	801:807	arg1	biomolecules					809:820	glycan binding biomolecules	794:820	glycan binding biomolecules	794:820	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	10	25	with	lectins	1727:1733	arg1	affinity					1746:1753	known affinity	1740:1753	known affinity towards terminal sialic acids	1740:1783	DLS and FCS confirmed that the glycans are accessible for binding as the corona interacts with lectins with known affinity towards terminal sialic acids and the enzymatic removal of the glycans leads to a decrease in lectin affinity.					
35066229	8	26	theme	FINDINGS	1420:1427	arg1	findings					1433:1440	FINDINGS Our findings	1420:1440	FINDINGS Our findings	1420:1440	FINDINGS Our findings highlighted that the protein corona composition is dependent on the exposing conditions.					
35066229	7	27	theme	Correlation	1291:1301	arg1	FCS					1317:1319	FCS	1317:1319	FCS	1317:1319	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	7	27	theme	Correlation	1291:1301	arg1	Spectroscopy					1303:1314	Fluorescence Correlation Spectroscopy	1278:1314	Fluorescence Correlation Spectroscopy (FCS)	1278:1320	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	6	28	theme	biomolecular	1111:1122	arg1	corona					1124:1129	the biomolecular corona	1107:1129	the biomolecular corona	1107:1129	We have extensively characterized the biomolecular corona using HILIC chromatography and shotgun proteomics.					
35066229	8	29	theme	exposing	1510:1517	arg1	conditions					1519:1528	the exposing conditions	1506:1528	the exposing conditions	1506:1528	FINDINGS Our findings highlighted that the protein corona composition is dependent on the exposing conditions.					
35066229	1	30	theme	blood	96:100	arg1	administration					102:115	blood administration	96:115	blood administration	96:115	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
35066229	1	30	theme	blood	96:100	arg1	surface					131:137	the pristine surface	118:137	the pristine surface of nanoparticles (NPs)	118:160	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
35066229	11	31	theme	biological	2017:2026	arg1	processes					2028:2036	NP biological processes	2014:2036	NP biological processes	2014:2036	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	2	32	theme	pristine	386:393	arg1	surface					395:401	the pristine surface	382:401	the pristine surface	382:401	It is well accepted that the biomolecular corona dramatically affects the NP fate in the biological medium while the pristine surface is no longer available for binding.					
35066229	2	32	theme	pristine	386:393	arg1	available					416:424	available	416:424	available	416:424	It is well accepted that the biomolecular corona dramatically affects the NP fate in the biological medium while the pristine surface is no longer available for binding.					
35066229	10	33	theme	lectin	1849:1854	arg1	affinity					1856:1863	lectin affinity	1849:1863	lectin affinity	1849:1863	DLS and FCS confirmed that the glycans are accessible for binding as the corona interacts with lectins with known affinity towards terminal sialic acids and the enzymatic removal of the glycans leads to a decrease in lectin affinity.					
35066229	4	34	with	interaction	745:755	arg1	lectins					831:837	lectins	831:837	lectins	831:837	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	4	34	with	interaction	745:755	arg1	biomolecules					809:820	glycan binding biomolecules	794:820	glycan binding biomolecules	794:820	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	4	35	from	role	716:719	arg1	interaction					745:755	the interaction	741:755	the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins	741:837	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	3	36	with	interaction	552:562	arg1	macrophages					569:579	macrophages	569:579	macrophages	569:579	Recent studies have shown that the glycans associated with the proteins forming the corona have a role in the NP interaction with macrophages, but the glycan identities remain unknown.					
35066229	6	37	theme	shotgun	1162:1168	arg1	proteomics					1170:1179	shotgun proteomics	1162:1179	shotgun proteomics	1162:1179	We have extensively characterized the biomolecular corona using HILIC chromatography and shotgun proteomics.					
35066229	11	38	theme	first	1891:1895	arg1	time					1897:1900	the first time	1887:1900	the first time	1887:1900	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	10	39	theme	sialic	1772:1777	arg1	acids					1779:1783	terminal sialic acids	1763:1783	terminal sialic acids	1763:1783	DLS and FCS confirmed that the glycans are accessible for binding as the corona interacts with lectins with known affinity towards terminal sialic acids and the enzymatic removal of the glycans leads to a decrease in lectin affinity.					
35066229	1	40	theme	surrounding	200:210	arg1	environment					212:222	the surrounding environment	196:222	the surrounding environment	196:222	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
35066229	0	41	theme	glycans	12:18	arg1	accessibility					20:32	the glycans accessibility	8:32	the glycans accessibility in the nanoparticle biomolecular corona	8:72	Probing the glycans accessibility in the nanoparticle biomolecular corona.					
35066229	5	42	dep	EXPERIMENTS	840:850	arg1	study					860:864	this study	855:864	this study	855:864	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	2	43	theme	biomolecular	298:309	arg1	corona					311:316	the biomolecular corona	294:316	the biomolecular corona	294:316	It is well accepted that the biomolecular corona dramatically affects the NP fate in the biological medium while the pristine surface is no longer available for binding.					
35066229	7	44	theme	Dynamic	1243:1249	arg1	DLS					1269:1271	DLS	1269:1271	DLS	1269:1271	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	7	44	theme	Dynamic	1243:1249	arg1	Scattering					1257:1266	Dynamic Light Scattering	1243:1266	Dynamic Light Scattering (DLS)	1243:1272	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	9	45	theme	plasma	1551:1556	arg1	concentrations					1558:1571	both plasma concentrations	1546:1571	both plasma concentrations	1546:1571	However, under both plasma concentrations, the biantennary sialylated glycans (A2G2S2) are enriched.					
35066229	11	46	theme	glycan	2071:2076	arg1	receptors					2086:2094	glycan binding receptors	2071:2094	glycan binding receptors that are highly expressed in an organism	2071:2135	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	7	47	theme	Light	1251:1255	arg1	DLS					1269:1271	DLS	1269:1271	DLS	1269:1271	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	7	47	theme	Light	1251:1255	arg1	Scattering					1257:1266	Dynamic Light Scattering	1243:1266	Dynamic Light Scattering (DLS)	1243:1272	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	7	48	theme	Fluorescence	1278:1289	arg1	FCS					1317:1319	FCS	1317:1319	FCS	1317:1319	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	7	48	theme	Fluorescence	1278:1289	arg1	Spectroscopy					1303:1314	Fluorescence Correlation Spectroscopy	1278:1314	Fluorescence Correlation Spectroscopy (FCS)	1278:1320	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	5	49	dep	in	1053:1054	arg1	vivo					1056:1059	vivo	1056:1059	vivo	1056:1059	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	5	50	theme	blood	973:977	arg1	plasma					979:984	blood plasma	973:984	blood plasma	973:984	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	0	51	theme	biomolecular	54:65	arg1	corona					67:72	the nanoparticle biomolecular corona	37:72	the nanoparticle biomolecular corona	37:72	Probing the glycans accessibility in the nanoparticle biomolecular corona.					
35066229	3	52	contain	have	530:533	arg1	glycans					474:480	the glycans	470:480	the glycans associated with the proteins forming the corona	470:528	Recent studies have shown that the glycans associated with the proteins forming the corona have a role in the NP interaction with macrophages, but the glycan identities remain unknown.					
35066229	3	52	contain	have	530:533	arg2	role					537:540	a role	535:540	a role	535:540	Recent studies have shown that the glycans associated with the proteins forming the corona have a role in the NP interaction with macrophages, but the glycan identities remain unknown.					
35066229	11	53	theme	NP	2014:2015	arg1	processes					2028:2036	NP biological processes	2014:2036	NP biological processes	2014:2036	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	5	54	theme	in	1040:1041	arg1	conditions					1061:1070	the in vitro and in vivo conditions	1036:1070	the in vitro and in vivo conditions	1036:1070	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	4	55	theme	glycan	794:799	arg1	lectins					831:837	lectins	831:837	lectins	831:837	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	4	55	theme	glycan	794:799	arg1	biomolecules					809:820	glycan binding biomolecules	794:820	glycan binding biomolecules	794:820	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	1	56	theme	pristine	122:129	arg1	administration					102:115	blood administration	96:115	blood administration	96:115	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
35066229	1	56	theme	pristine	122:129	arg1	surface					131:137	the pristine surface	118:137	the pristine surface of nanoparticles (NPs)	118:160	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
35066229	0	57	theme	nanoparticle	41:52	arg1	corona					67:72	the nanoparticle biomolecular corona	37:72	the nanoparticle biomolecular corona	37:72	Probing the glycans accessibility in the nanoparticle biomolecular corona.					
35066229	6	58	theme	HILIC	1137:1141	arg1	chromatography					1143:1156	HILIC chromatography	1137:1156	HILIC chromatography	1137:1156	We have extensively characterized the biomolecular corona using HILIC chromatography and shotgun proteomics.					
35066229	10	59	theme	enzymatic	1793:1801	arg1	removal					1803:1809	the enzymatic removal	1789:1809	the enzymatic removal of the glycans	1789:1824	DLS and FCS confirmed that the glycans are accessible for binding as the corona interacts with lectins with known affinity towards terminal sialic acids and the enzymatic removal of the glycans leads to a decrease in lectin affinity.					
35066229	7	60	theme	known	1354:1358	arg1	affinity					1360:1367	known affinity	1354:1367	known affinity towards specific glycans	1354:1392	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	4	61	from	corona	782:787	arg1	proteins					764:771	the proteins	760:771	the proteins from the corona	760:787	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	4	61	from	corona	782:787	arg1	interaction					745:755	the interaction	741:755	the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins	741:837	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	4	62	theme	corona	691:696	arg1	composition					659:669	the glycan composition	648:669	the glycan composition of the biomolecular corona	648:696	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	5	63	theme	NPs	966:968	arg1	exposure					950:957	exposure	950:957	exposure of the NPs to blood plasma	950:984	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	5	64	theme	different	993:1001	arg1	concentrations					1010:1023	two different plasma concentrations	989:1023	two different plasma concentrations	989:1023	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	9	65	theme	biantennary	1578:1588	arg1	A2G2S2					1610:1615	A2G2S2	1610:1615	A2G2S2	1610:1615	However, under both plasma concentrations, the biantennary sialylated glycans (A2G2S2) are enriched.					
35066229	9	65	theme	biantennary	1578:1588	arg1	glycans					1601:1607	the biantennary sialylated glycans	1574:1607	the biantennary sialylated glycans (A2G2S2)	1574:1616	However, under both plasma concentrations, the biantennary sialylated glycans (A2G2S2) are enriched.					
35066229	1	66	from	environment	212:222	arg1	biomolecules					178:189	biomolecules	178:189	biomolecules from the surrounding environment	178:222	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
35066229	2	67	theme	NP	343:344	arg1	fate					346:349	the NP fate	339:349	the NP fate	339:349	It is well accepted that the biomolecular corona dramatically affects the NP fate in the biological medium while the pristine surface is no longer available for binding.					
35066229	1	68	theme	so-called	236:244	arg1	"					266:266	the so-called "biomolecular corona"	232:266	the so-called "biomolecular corona"	232:266	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
35066229	5	69	theme	plasma	1003:1008	arg1	concentrations					1010:1023	two different plasma concentrations	989:1023	two different plasma concentrations	989:1023	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	9	70	theme	sialylated	1590:1599	arg1	A2G2S2					1610:1615	A2G2S2	1610:1615	A2G2S2	1610:1615	However, under both plasma concentrations, the biantennary sialylated glycans (A2G2S2) are enriched.					
35066229	9	70	theme	sialylated	1590:1599	arg1	glycans					1601:1607	the biantennary sialylated glycans	1574:1607	the biantennary sialylated glycans (A2G2S2)	1574:1616	However, under both plasma concentrations, the biantennary sialylated glycans (A2G2S2) are enriched.					
35066229	1	71	theme	nanoparticles	142:154	arg1	administration					102:115	blood administration	96:115	blood administration	96:115	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
35066229	1	71	theme	nanoparticles	142:154	arg1	surface					131:137	the pristine surface	118:137	the pristine surface of nanoparticles (NPs)	118:160	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
35066229	11	72	theme	binding	2078:2084	arg1	receptors					2086:2094	glycan binding receptors	2071:2094	glycan binding receptors that are highly expressed in an organism	2071:2135	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	5	73	dep	in	1040:1041	arg1	vitro					1043:1047	vitro	1043:1047	vitro	1043:1047	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	11	74	attach	present	1923:1929	arg1	corona					1938:1943	the corona	1934:1943	the corona	1934:1943	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	11	74	attach	present	1923:1929	arg2	present					1923:1929	present	1923:1929	present	1923:1929	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	11	74	attach	present	1923:1929	arg2	glycans					1911:1917	the glycans	1907:1917	the glycans	1907:1917	This study shows for the first time that the glycans are present in the corona and that they could potentially be responsible for the modulation of NP biological processes as they can directly engage with glycan binding receptors that are highly expressed in an organism.					
35066229	3	75	theme	glycan	590:595	arg1	identities					597:606	the glycan identities	586:606	the glycan identities	586:606	Recent studies have shown that the glycans associated with the proteins forming the corona have a role in the NP interaction with macrophages, but the glycan identities remain unknown.					
35066229	5	76	theme	citrate	916:922	arg1	corona					906:911	the biomolecular corona	889:911	the biomolecular corona of citrate	889:922	EXPERIMENTS In this study, we have characterized the biomolecular corona of citrate stabilised gold NPs after exposure of the NPs to blood plasma at two different plasma concentrations, mimicking the in vitro and in vivo conditions.					
35066229	1	77	theme	biomolecular	247:258	arg1	"					266:266	the so-called "biomolecular corona"	232:266	the so-called "biomolecular corona"	232:266	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
35066229	0	78	from	accessibility	20:32	arg1	corona					67:72	the nanoparticle biomolecular corona	37:72	the nanoparticle biomolecular corona	37:72	Probing the glycans accessibility in the nanoparticle biomolecular corona.					
35066229	4	79	theme	proteins	764:771	arg1	interaction					745:755	the interaction	741:755	the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins	741:837	We aim here to identify the glycan composition of the biomolecular corona and to assess the role of these glycans in the interaction of the proteins from the corona with glycan binding biomolecules, such as lectins.					
35066229	7	80	theme	lectin	1200:1205	arg1	assay					1215:1219	a lectin binding assay	1198:1219	a lectin binding assay	1198:1219	Following this, a lectin binding assay was carried out using Dynamic Light Scattering (DLS) and Fluorescence Correlation Spectroscopy (FCS) to assess whether proteins with known affinity towards specific glycans would bind to the corona.					
35066229	1	81	theme	corona	260:265	arg1	"					266:266	the so-called "biomolecular corona"	232:266	the so-called "biomolecular corona"	232:266	HYPOTHESIS Following blood administration, the pristine surface of nanoparticles (NPs) associates with biomolecules from the surrounding environment forming the so-called "biomolecular corona".					
34339169	4	0	theme	trimeric	683:690	arg1	adhesin					708:714	a trimeric autotransporter adhesin	681:714	a trimeric autotransporter adhesin known as Ata that has been previously demonstrated to bind components of the host cell's extracellular matrix, which are often heavily glycosylated	681:862	A. baumannii produces a trimeric autotransporter adhesin known as Ata that has been previously demonstrated to bind components of the host cell's extracellular matrix, which are often heavily glycosylated.					
34339169	3	1	theme	A.	507:508	arg1	factors					520:526	many A. baumannii factors	502:526	many A. baumannii factors required to colonize the host	502:556	Although many A. baumannii factors required to colonize the host have been identified, little is known about the specific host molecules recognized by these factors.					
34339169	1	2	theme	tissues	249:255	arg1	infections					188:197	infections	188:197	infections of the lung, blood, skin, urinary tract, and soft tissues	188:255	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	0	3	theme	High-Affinity	83:95	arg1	Glycans					72:78	Host Glycans	67:78	Host Glycans	67:78	The Acinetobacter baumannii Autotransporter Adhesin Ata Recognizes Host Glycans as High-Affinity Receptors.					
34339169	0	3	theme	High-Affinity	83:95	arg1	Receptors					97:105	High-Affinity Receptors	83:105	High-Affinity Receptors	83:105	The Acinetobacter baumannii Autotransporter Adhesin Ata Recognizes Host Glycans as High-Affinity Receptors.					
34339169	4	4	theme	autotransporter	692:706	arg1	adhesin					708:714	a trimeric autotransporter adhesin	681:714	a trimeric autotransporter adhesin known as Ata that has been previously demonstrated to bind components of the host cell's extracellular matrix, which are often heavily glycosylated	681:862	A. baumannii produces a trimeric autotransporter adhesin known as Ata that has been previously demonstrated to bind components of the host cell's extracellular matrix, which are often heavily glycosylated.					
34339169	10	5	theme	novel	1718:1722	arg1	compounds					1724:1732	novel compounds	1718:1732	novel compounds	1718:1732	This information will assist in directing the development of new and effective treatments to block host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections.					
34339169	8	6	theme	fibronectin	1343:1353	arg1	recognition					1315:1325	the recognition	1311:1325	the recognition of human plasma fibronectin by Ata	1311:1360	We also demonstrated that the recognition of human plasma fibronectin by Ata requires this ability to bind glycans, as the interaction between Ata and fibronectin does not occur when fibronectin is deglycosylated.					
34339169	9	7	theme	Ata	1537:1539	arg1	activity					1548:1555	Ata lectin activity	1537:1555	Ata lectin activity	1537:1555	This strongly suggests a key role for Ata lectin activity during host adherence.					
34339169	8	8	theme	plasma	1336:1341	arg1	fibronectin					1343:1353	human plasma fibronectin	1330:1353	human plasma fibronectin	1330:1353	We also demonstrated that the recognition of human plasma fibronectin by Ata requires this ability to bind glycans, as the interaction between Ata and fibronectin does not occur when fibronectin is deglycosylated.					
34339169	6	9	with	galactose	1086:1094	arg1	high-affinity					1166:1178	high-affinity	1166:1178	high-affinity	1166:1178	Our biophysical analysis using glycan arrays and surface plasmon resonance demonstrated that Ata binds galactose, N-acetylglucosamine, and galactose (β1-3/4) N-acetylglucosamine with high-affinity.					
34339169	6	10	theme	plasmon	1040:1046	arg1	resonance					1048:1056	surface plasmon resonance	1032:1056	surface plasmon resonance	1032:1056	Our biophysical analysis using glycan arrays and surface plasmon resonance demonstrated that Ata binds galactose, N-acetylglucosamine, and galactose (β1-3/4) N-acetylglucosamine with high-affinity.					
34339169	5	11	theme	host	964:967	arg1	surface					974:980	the host cell surface	960:980	the host cell surface	960:980	We hypothesized that Ata would exhibit lectin activity which would play a role in adherence to the host cell surface.					
34339169	10	12	theme	new	1641:1643	arg1	treatments					1659:1668	new and effective treatments	1641:1668	new and effective treatments	1641:1668	This information will assist in directing the development of new and effective treatments to block host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections.					
34339169	2	13	theme	new	397:399	arg1	development					412:422	new antibiotic development	397:422	new antibiotic development	397:422	This multidrug resistance, together with a dearth of new antibiotic development, mean novel methods of treatment and prevention are urgently needed.					
34339169	5	14	theme	lectin	904:909	arg1	activity					911:918	lectin activity	904:918	lectin activity which would play a role in adherence to the host cell surface	904:980	We hypothesized that Ata would exhibit lectin activity which would play a role in adherence to the host cell surface.					
34339169	1	15	with	pathogen	163:170	arg1	strains					268:274	some strains	263:274	some strains exhibiting almost complete resistance to commonly used antibiotics	263:341	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	5	16	theme	cell	969:972	arg1	surface					974:980	the host cell surface	960:980	the host cell surface	960:980	We hypothesized that Ata would exhibit lectin activity which would play a role in adherence to the host cell surface.					
34339169	1	17	theme	lung	206:209	arg1	infections					188:197	infections	188:197	infections of the lung, blood, skin, urinary tract, and soft tissues	188:255	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	0	18	theme	Acinetobacter	4:16	arg1	Ata					52:54	The Acinetobacter baumannii Autotransporter Adhesin Ata	0:54	The Acinetobacter baumannii Autotransporter Adhesin Ata	0:54	The Acinetobacter baumannii Autotransporter Adhesin Ata Recognizes Host Glycans as High-Affinity Receptors.					
34339169	6	19	with	N-acetylglucosamine	1097:1115	arg1	high-affinity					1166:1178	high-affinity	1166:1178	high-affinity	1166:1178	Our biophysical analysis using glycan arrays and surface plasmon resonance demonstrated that Ata binds galactose, N-acetylglucosamine, and galactose (β1-3/4) N-acetylglucosamine with high-affinity.					
34339169	7	20	theme	proteins	1225:1232	arg1	many					1213:1216	many	1213:1216	many	1213:1216	These structures are present on many of the proteins which were previously reported to be bound by Ata.					
34339169	7	20	theme	proteins	1225:1232	arg1	proteins					1225:1232	the proteins	1221:1232	the proteins which were previously reported to be bound by Ata	1221:1282	These structures are present on many of the proteins which were previously reported to be bound by Ata.					
34339169	3	21	dep	A.	507:508	arg1	baumannii					510:518	baumannii	510:518	baumannii	510:518	Although many A. baumannii factors required to colonize the host have been identified, little is known about the specific host molecules recognized by these factors.					
34339169	10	22	theme	A.	1757:1758	arg1	infections					1770:1779	multidrug resistant A. baumannii infections	1737:1779	multidrug resistant A. baumannii infections	1737:1779	This information will assist in directing the development of new and effective treatments to block host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections.					
34339169	1	23	theme	Acinetobacter	108:120	arg1	pathogen					163:170	a significant opportunistic pathogen	135:170	a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues	135:255	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	1	23	theme	Acinetobacter	108:120	arg1	baumannii					122:130	Acinetobacter baumannii	108:130	Acinetobacter baumannii	108:130	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	6	24	theme	biophysical	987:997	arg1	analysis					999:1006	Our biophysical analysis	983:1006	Our biophysical analysis using glycan arrays and surface plasmon resonance	983:1056	Our biophysical analysis using glycan arrays and surface plasmon resonance demonstrated that Ata binds galactose, N-acetylglucosamine, and galactose (β1-3/4) N-acetylglucosamine with high-affinity.					
34339169	1	25	theme	blood	212:216	arg1	infections					188:197	infections	188:197	infections of the lung, blood, skin, urinary tract, and soft tissues	188:255	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	0	26	theme	Autotransporter	28:42	arg1	Ata					52:54	The Acinetobacter baumannii Autotransporter Adhesin Ata	0:54	The Acinetobacter baumannii Autotransporter Adhesin Ata	0:54	The Acinetobacter baumannii Autotransporter Adhesin Ata Recognizes Host Glycans as High-Affinity Receptors.					
34339169	10	27	from	glycans	1703:1709	arg1	infections					1770:1779	multidrug resistant A. baumannii infections	1737:1779	multidrug resistant A. baumannii infections	1737:1779	This information will assist in directing the development of new and effective treatments to block host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections.					
34339169	8	28	gly	deglycosylated	1483:1496	arg1	fibronectin					1468:1478	fibronectin	1468:1478	fibronectin	1468:1478	We also demonstrated that the recognition of human plasma fibronectin by Ata requires this ability to bind glycans, as the interaction between Ata and fibronectin does not occur when fibronectin is deglycosylated.					
34339169	2	29	theme	mean	425:428	arg1	methods					436:442	mean novel methods	425:442	mean novel methods of treatment and prevention	425:470	This multidrug resistance, together with a dearth of new antibiotic development, mean novel methods of treatment and prevention are urgently needed.					
34339169	2	30	theme	novel	430:434	arg1	methods					436:442	mean novel methods	425:442	mean novel methods of treatment and prevention	425:470	This multidrug resistance, together with a dearth of new antibiotic development, mean novel methods of treatment and prevention are urgently needed.					
34339169	9	31	theme	lectin	1541:1546	arg1	activity					1548:1555	Ata lectin activity	1537:1555	Ata lectin activity	1537:1555	This strongly suggests a key role for Ata lectin activity during host adherence.					
34339169	4	32	gly	glycosylated	851:862	arg1	components					775:784	components	775:784	components	775:784	A. baumannii produces a trimeric autotransporter adhesin known as Ata that has been previously demonstrated to bind components of the host cell's extracellular matrix, which are often heavily glycosylated.					
34339169	3	33	theme	many	502:505	arg1	factors					520:526	many A. baumannii factors	502:526	many A. baumannii factors required to colonize the host	502:556	Although many A. baumannii factors required to colonize the host have been identified, little is known about the specific host molecules recognized by these factors.					
34339169	10	34	theme	baumannii	1760:1768	arg1	infections					1770:1779	multidrug resistant A. baumannii infections	1737:1779	multidrug resistant A. baumannii infections	1737:1779	This information will assist in directing the development of new and effective treatments to block host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections.					
34339169	1	35	theme	skin	219:222	arg1	infections					188:197	infections	188:197	infections of the lung, blood, skin, urinary tract, and soft tissues	188:255	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	1	36	theme	complete	294:301	arg1	resistance					303:312	almost complete resistance	287:312	almost complete resistance to commonly used antibiotics	287:341	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	6	37	theme	surface	1032:1038	arg1	resonance					1048:1056	surface plasmon resonance	1032:1056	surface plasmon resonance	1032:1056	Our biophysical analysis using glycan arrays and surface plasmon resonance demonstrated that Ata binds galactose, N-acetylglucosamine, and galactose (β1-3/4) N-acetylglucosamine with high-affinity.					
34339169	4	38	theme	matrix	819:824	arg1	components					775:784	components	775:784	components	775:784	A. baumannii produces a trimeric autotransporter adhesin known as Ata that has been previously demonstrated to bind components of the host cell's extracellular matrix, which are often heavily glycosylated.					
34339169	2	39	theme	development	412:422	arg1	dearth					387:392	a dearth	385:392	a dearth of new antibiotic development	385:422	This multidrug resistance, together with a dearth of new antibiotic development, mean novel methods of treatment and prevention are urgently needed.					
34339169	0	40	theme	Adhesin	44:50	arg1	Ata					52:54	The Acinetobacter baumannii Autotransporter Adhesin Ata	0:54	The Acinetobacter baumannii Autotransporter Adhesin Ata	0:54	The Acinetobacter baumannii Autotransporter Adhesin Ata Recognizes Host Glycans as High-Affinity Receptors.					
34339169	6	41	with	N-acetylglucosamine	1141:1159	arg1	high-affinity					1166:1178	high-affinity	1166:1178	high-affinity	1166:1178	Our biophysical analysis using glycan arrays and surface plasmon resonance demonstrated that Ata binds galactose, N-acetylglucosamine, and galactose (β1-3/4) N-acetylglucosamine with high-affinity.					
34339169	4	42	theme	extracellular	805:817	arg1	matrix					819:824	the host cell's extracellular matrix	789:824	the host cell's extracellular matrix	789:824	A. baumannii produces a trimeric autotransporter adhesin known as Ata that has been previously demonstrated to bind components of the host cell's extracellular matrix, which are often heavily glycosylated.					
34339169	1	43	theme	urinary	225:231	arg1	tract					233:237	urinary tract	225:237	urinary tract	225:237	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	0	44	theme	Host	67:70	arg1	Glycans					72:78	Host Glycans	67:78	Host Glycans	67:78	The Acinetobacter baumannii Autotransporter Adhesin Ata Recognizes Host Glycans as High-Affinity Receptors.					
34339169	0	44	theme	Host	67:70	arg1	Receptors					97:105	High-Affinity Receptors	83:105	High-Affinity Receptors	83:105	The Acinetobacter baumannii Autotransporter Adhesin Ata Recognizes Host Glycans as High-Affinity Receptors.					
34339169	9	45	theme	key	1524:1526	arg1	role					1528:1531	a key role	1522:1531	a key role for Ata lectin activity during host adherence	1522:1577	This strongly suggests a key role for Ata lectin activity during host adherence.					
34339169	2	46	theme	multidrug	349:357	arg1	resistance					359:368	This multidrug resistance	344:368	This multidrug resistance	344:368	This multidrug resistance, together with a dearth of new antibiotic development, mean novel methods of treatment and prevention are urgently needed.					
34339169	10	47	theme	resistant	1747:1755	arg1	infections					1770:1779	multidrug resistant A. baumannii infections	1737:1779	multidrug resistant A. baumannii infections	1737:1779	This information will assist in directing the development of new and effective treatments to block host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections.					
34339169	1	48	dep	lung	206:209	arg1	the					202:204	the	202:204	the	202:204	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	1	49	theme	significant	137:147	arg1	pathogen					163:170	a significant opportunistic pathogen	135:170	a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues	135:255	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	1	49	theme	significant	137:147	arg1	baumannii					122:130	Acinetobacter baumannii	108:130	Acinetobacter baumannii	108:130	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	1	50	theme	used	326:329	arg1	antibiotics					331:341	commonly used antibiotics	317:341	commonly used antibiotics	317:341	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	4	51	theme	host	793:796	arg1	cell					798:801	the host cell's	789:803	the host cell's extracellular matrix	789:824	A. baumannii produces a trimeric autotransporter adhesin known as Ata that has been previously demonstrated to bind components of the host cell's extracellular matrix, which are often heavily glycosylated.					
34339169	3	52	theme	host	615:618	arg1	molecules					620:628	the specific host molecules	602:628	the specific host molecules recognized by these factors	602:656	Although many A. baumannii factors required to colonize the host have been identified, little is known about the specific host molecules recognized by these factors.					
34339169	6	53	theme	glycan	1014:1019	arg1	arrays					1021:1026	glycan arrays	1014:1026	glycan arrays	1014:1026	Our biophysical analysis using glycan arrays and surface plasmon resonance demonstrated that Ata binds galactose, N-acetylglucosamine, and galactose (β1-3/4) N-acetylglucosamine with high-affinity.					
34339169	1	54	theme	opportunistic	149:161	arg1	pathogen					163:170	a significant opportunistic pathogen	135:170	a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues	135:255	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	1	54	theme	opportunistic	149:161	arg1	baumannii					122:130	Acinetobacter baumannii	108:130	Acinetobacter baumannii	108:130	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	10	55	theme	multidrug	1737:1745	arg1	infections					1770:1779	multidrug resistant A. baumannii infections	1737:1779	multidrug resistant A. baumannii infections	1737:1779	This information will assist in directing the development of new and effective treatments to block host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections.					
34339169	1	56	theme	tract	233:237	arg1	infections					188:197	infections	188:197	infections of the lung, blood, skin, urinary tract, and soft tissues	188:255	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	7	57	attach	present	1202:1208	arg1	many					1213:1216	many	1213:1216	many	1213:1216	These structures are present on many of the proteins which were previously reported to be bound by Ata.					
34339169	7	57	attach	present	1202:1208	arg1	proteins					1225:1232	the proteins	1221:1232	the proteins which were previously reported to be bound by Ata	1221:1282	These structures are present on many of the proteins which were previously reported to be bound by Ata.					
34339169	7	57	attach	present	1202:1208	arg2	structures					1187:1196	These structures	1181:1196	These structures	1181:1196	These structures are present on many of the proteins which were previously reported to be bound by Ata.					
34339169	10	58	theme	treatments	1659:1668	arg1	development					1626:1636	the development	1622:1636	the development of new and effective treatments	1622:1668	This information will assist in directing the development of new and effective treatments to block host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections.					
34339169	7	59	located	present	1202:1208	arg1	many					1213:1216	many	1213:1216	many	1213:1216	These structures are present on many of the proteins which were previously reported to be bound by Ata.					
34339169	7	59	located	present	1202:1208	arg1	proteins					1225:1232	the proteins	1221:1232	the proteins which were previously reported to be bound by Ata	1221:1282	These structures are present on many of the proteins which were previously reported to be bound by Ata.					
34339169	7	59	located	present	1202:1208	arg2	structures					1187:1196	These structures	1181:1196	These structures	1181:1196	These structures are present on many of the proteins which were previously reported to be bound by Ata.					
34339169	8	60	theme	human	1330:1334	arg1	fibronectin					1343:1353	human plasma fibronectin	1330:1353	human plasma fibronectin	1330:1353	We also demonstrated that the recognition of human plasma fibronectin by Ata requires this ability to bind glycans, as the interaction between Ata and fibronectin does not occur when fibronectin is deglycosylated.					
34339169	6	61	theme	galactose	1122:1130	arg1	N-acetylglucosamine					1141:1159	galactose (β1-3/4) N-acetylglucosamine	1122:1159	galactose (β1-3/4) N-acetylglucosamine	1122:1159	Our biophysical analysis using glycan arrays and surface plasmon resonance demonstrated that Ata binds galactose, N-acetylglucosamine, and galactose (β1-3/4) N-acetylglucosamine with high-affinity.					
34339169	2	62	theme	antibiotic	401:410	arg1	development					412:422	new antibiotic development	397:422	new antibiotic development	397:422	This multidrug resistance, together with a dearth of new antibiotic development, mean novel methods of treatment and prevention are urgently needed.					
34339169	0	63	dep	Acinetobacter	4:16	arg1	baumannii					18:26	baumannii	18:26	baumannii	18:26	The Acinetobacter baumannii Autotransporter Adhesin Ata Recognizes Host Glycans as High-Affinity Receptors.					
34339169	10	64	theme	host	1679:1682	arg1	interactions					1684:1695	host interactions	1679:1695	host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections	1679:1779	This information will assist in directing the development of new and effective treatments to block host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections.					
34339169	1	65	theme	responsible	172:182	arg1	pathogen					163:170	a significant opportunistic pathogen	135:170	a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues	135:255	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	1	65	theme	responsible	172:182	arg1	baumannii					122:130	Acinetobacter baumannii	108:130	Acinetobacter baumannii	108:130	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34339169	9	66	theme	host	1564:1567	arg1	adherence					1569:1577	host adherence	1564:1577	host adherence	1564:1577	This strongly suggests a key role for Ata lectin activity during host adherence.					
34339169	10	67	theme	effective	1649:1657	arg1	treatments					1659:1668	new and effective treatments	1641:1668	new and effective treatments	1641:1668	This information will assist in directing the development of new and effective treatments to block host interactions using glycans and/or novel compounds in multidrug resistant A. baumannii infections.					
34339169	2	68	theme	prevention	461:470	arg1	methods					436:442	mean novel methods	425:442	mean novel methods of treatment and prevention	425:470	This multidrug resistance, together with a dearth of new antibiotic development, mean novel methods of treatment and prevention are urgently needed.					
34339169	3	69	theme	specific	606:613	arg1	molecules					620:628	the specific host molecules	602:628	the specific host molecules recognized by these factors	602:656	Although many A. baumannii factors required to colonize the host have been identified, little is known about the specific host molecules recognized by these factors.					
34339169	2	70	theme	treatment	447:455	arg1	methods					436:442	mean novel methods	425:442	mean novel methods of treatment and prevention	425:470	This multidrug resistance, together with a dearth of new antibiotic development, mean novel methods of treatment and prevention are urgently needed.					
34339169	1	71	theme	soft	244:247	arg1	tissues					249:255	soft tissues	244:255	soft tissues	244:255	Acinetobacter baumannii is a significant opportunistic pathogen responsible for infections of the lung, blood, skin, urinary tract, and soft tissues, with some strains exhibiting almost complete resistance to commonly used antibiotics.					
34004145	4	0	theme	small	439:443	arg1	RNAs					455:458	conserved small noncoding RNAs	429:458	conserved small noncoding RNAs	429:458	Using a battery of chemical and biochemical approaches, we found that conserved small noncoding RNAs bear sialylated glycans.					
34004145	1	1	theme	inter-	131:136	arg1	interactions					157:168	inter- and intramolecular interactions	131:168	inter- and intramolecular interactions	131:168	Glycans modify lipids and proteins to mediate inter- and intramolecular interactions across all domains of life.					
34004145	7	2	theme	receptor	895:902	arg1	family					904:909	the Siglec receptor family	884:909	the Siglec receptor family	884:909	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	8	3	theme	direct	968:973	arg1	interface					975:983	a direct interface	966:983	a direct interface between RNA biology and glycobiology	966:1020	Collectively, these findings suggest the existence of a direct interface between RNA biology and glycobiology, and an expanded role for RNA in extracellular biology.					
34004145	0	4	theme	cells	78:82	arg1	surface					60:66	the surface	56:66	the surface of living cells	56:82	Small RNAs are modified with N-glycans and displayed on the surface of living cells.					
34004145	8	5	theme	interface	975:983	arg1	role					1039:1042	an expanded role	1027:1042	an expanded role for RNA in extracellular biology	1027:1075	Collectively, these findings suggest the existence of a direct interface between RNA biology and glycobiology, and an expanded role for RNA in extracellular biology.					
34004145	8	5	theme	interface	975:983	arg1	existence					953:961	the existence	949:961	the existence of a direct interface between RNA biology and glycobiology	949:1020	Collectively, these findings suggest the existence of a direct interface between RNA biology and glycobiology, and an expanded role for RNA in extracellular biology.					
34004145	7	6	theme	family	904:909	arg1	members					873:879	members	873:879	members of the Siglec receptor family	873:909	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	7	6	theme	family	904:909	arg1	antibodies					858:867	anti-dsRNA antibodies	847:867	anti-dsRNA antibodies	847:867	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	7	7	theme	cell	812:815	arg1	surface					817:823	the cell surface	808:823	the cell surface	808:823	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	8	8	from	role	1039:1042	arg1	biology					1069:1075	extracellular biology	1055:1075	extracellular biology	1055:1075	Collectively, these findings suggest the existence of a direct interface between RNA biology and glycobiology, and an expanded role for RNA in extracellular biology.					
34004145	0	9	theme	living	71:76	arg1	cells					78:82	living cells	71:82	living cells	71:82	Small RNAs are modified with N-glycans and displayed on the surface of living cells.					
34004145	6	10	theme	sialic	703:708	arg1	acid					710:713	sialic acid	703:713	sialic acid	703:713	GlycoRNA assembly depends on canonical N-glycan biosynthetic machinery and results in structures enriched in sialic acid and fucose.					
34004145	5	11	theme	mammalian	543:551	arg1	species					553:559	mammalian species	543:559	mammalian species	543:559	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	2	12	theme	glycosylation	241:253	arg1	target					231:236	a major target	223:236	a major target of glycosylation	223:253	RNA is not thought to be a major target of glycosylation.					
34004145	2	12	theme	glycosylation	241:253	arg1	RNA					198:200	RNA	198:200	RNA	198:200	RNA is not thought to be a major target of glycosylation.					
34004145	7	13	located	present	797:803	arg1	surface					817:823	the cell surface	808:823	the cell surface	808:823	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	7	13	located	present	797:803	arg2	present					797:803	present	797:803	present	797:803	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	7	13	located	present	797:803	arg2	majority					770:777	the majority	766:777	the majority of glycoRNAs	766:790	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	8	14	theme	RNA	993:995	arg1	biology					997:1003	RNA biology	993:1003	RNA biology	993:1003	Collectively, these findings suggest the existence of a direct interface between RNA biology and glycobiology, and an expanded role for RNA in extracellular biology.					
34004145	7	15	theme	Siglec	888:893	arg1	family					904:909	the Siglec receptor family	884:909	the Siglec receptor family	884:909	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	8	16	theme	expanded	1030:1037	arg1	role					1039:1042	an expanded role	1027:1042	an expanded role for RNA in extracellular biology	1027:1075	Collectively, these findings suggest the existence of a direct interface between RNA biology and glycobiology, and an expanded role for RNA in extracellular biology.					
34004145	6	17	theme	biosynthetic	642:653	arg1	machinery					655:663	canonical N-glycan biosynthetic machinery	623:663	canonical N-glycan biosynthetic machinery	623:663	GlycoRNA assembly depends on canonical N-glycan biosynthetic machinery and results in structures enriched in sialic acid and fucose.					
34004145	1	18	theme	intramolecular	142:155	arg1	interactions					157:168	inter- and intramolecular interactions	131:168	inter- and intramolecular interactions	131:168	Glycans modify lipids and proteins to mediate inter- and intramolecular interactions across all domains of life.					
34004145	4	19	theme	conserved	429:437	arg1	RNAs					455:458	conserved small noncoding RNAs	429:458	conserved small noncoding RNAs	429:458	Using a battery of chemical and biochemical approaches, we found that conserved small noncoding RNAs bear sialylated glycans.					
34004145	6	20	theme	N-glycan	633:640	arg1	machinery					655:663	canonical N-glycan biosynthetic machinery	623:663	canonical N-glycan biosynthetic machinery	623:663	GlycoRNA assembly depends on canonical N-glycan biosynthetic machinery and results in structures enriched in sialic acid and fucose.					
34004145	3	21	theme	third	325:329	arg1	scaffold					331:338	a third scaffold	323:338	a third scaffold for glycosylation	323:356	Here, we challenge this view with evidence that mammals use RNA as a third scaffold for glycosylation.					
34004145	3	21	theme	third	325:329	arg1	RNA					316:318	RNA	316:318	RNA	316:318	Here, we challenge this view with evidence that mammals use RNA as a third scaffold for glycosylation.					
34004145	5	22	theme	cultured	565:572	arg1	cells					574:578	cultured cells	565:578	cultured cells	565:578	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	6	23	theme	canonical	623:631	arg1	machinery					655:663	canonical N-glycan biosynthetic machinery	623:663	canonical N-glycan biosynthetic machinery	623:663	GlycoRNA assembly depends on canonical N-glycan biosynthetic machinery and results in structures enriched in sialic acid and fucose.					
34004145	0	24	theme	Small	0:4	arg1	RNAs					6:9	Small RNAs	0:9	Small RNAs	0:9	Small RNAs are modified with N-glycans and displayed on the surface of living cells.					
34004145	7	25	theme	cells	746:750	arg1	Analysis					727:734	Analysis	727:734	Analysis of living cells	727:750	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	5	26	theme	multiple	519:526	arg1	types					533:537	multiple cell types	519:537	multiple cell types	519:537	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	3	27	with	challenge	265:273	arg1	evidence					290:297	evidence	290:297	evidence that mammals use RNA as a third scaffold for glycosylation	290:356	Here, we challenge this view with evidence that mammals use RNA as a third scaffold for glycosylation.					
34004145	5	28	theme	cell	528:531	arg1	types					533:537	multiple cell types	519:537	multiple cell types	519:537	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	4	29	theme	biochemical	391:401	arg1	approaches					403:412	chemical and biochemical approaches	378:412	chemical and biochemical approaches	378:412	Using a battery of chemical and biochemical approaches, we found that conserved small noncoding RNAs bear sialylated glycans.					
34004145	7	30	theme	living	739:744	arg1	cells					746:750	living cells	739:750	living cells	739:750	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	6	31	theme	GlycoRNA	594:601	arg1	assembly					603:610	GlycoRNA assembly	594:610	GlycoRNA assembly	594:610	GlycoRNA assembly depends on canonical N-glycan biosynthetic machinery and results in structures enriched in sialic acid and fucose.					
34004145	1	32	theme	life	192:195	arg1	life					192:195	life	192:195	life	192:195	Glycans modify lipids and proteins to mediate inter- and intramolecular interactions across all domains of life.					
34004145	1	32	theme	life	192:195	arg1	domains					181:187	all domains	177:187	all domains of life	177:195	Glycans modify lipids and proteins to mediate inter- and intramolecular interactions across all domains of life.					
34004145	5	33	attach	present	508:514	arg2	"					501:501	These "glycoRNAs"	485:501	These "glycoRNAs"	485:501	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	5	33	attach	present	508:514	arg1	types					533:537	multiple cell types	519:537	multiple cell types	519:537	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	5	33	attach	present	508:514	arg1	species					553:559	mammalian species	543:559	mammalian species	543:559	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	3	34	dep	evidence	290:297	arg1	use					312:314	use	312:314	use RNA as a third scaffold for glycosylation	312:356	Here, we challenge this view with evidence that mammals use RNA as a third scaffold for glycosylation.					
34004145	4	35	theme	sialylated	465:474	arg1	glycans					476:482	sialylated glycans	465:482	sialylated glycans	465:482	Using a battery of chemical and biochemical approaches, we found that conserved small noncoding RNAs bear sialylated glycans.					
34004145	4	36	theme	approaches	403:412	arg1	battery					367:373	a battery	365:373	a battery of chemical and biochemical approaches	365:412	Using a battery of chemical and biochemical approaches, we found that conserved small noncoding RNAs bear sialylated glycans.					
34004145	5	37	theme	glycoRNAs	492:500	arg1	"					501:501	These "glycoRNAs"	485:501	These "glycoRNAs"	485:501	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	7	38	attach	present	797:803	arg1	surface					817:823	the cell surface	808:823	the cell surface	808:823	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	7	38	attach	present	797:803	arg2	present					797:803	present	797:803	present	797:803	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	7	38	attach	present	797:803	arg2	majority					770:777	the majority	766:777	the majority of glycoRNAs	766:790	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	5	39	located	present	508:514	arg2	"					501:501	These "glycoRNAs"	485:501	These "glycoRNAs"	485:501	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	5	39	located	present	508:514	arg1	types					533:537	multiple cell types	519:537	multiple cell types	519:537	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	5	39	located	present	508:514	arg1	species					553:559	mammalian species	543:559	mammalian species	543:559	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	5	40	from	types	533:537	arg1	present					508:514	present	508:514	present	508:514	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	2	41	theme	major	225:229	arg1	target					231:236	a major target	223:236	a major target of glycosylation	223:253	RNA is not thought to be a major target of glycosylation.					
34004145	2	41	theme	major	225:229	arg1	RNA					198:200	RNA	198:200	RNA	198:200	RNA is not thought to be a major target of glycosylation.					
34004145	5	42	from	present	508:514	arg1	types					533:537	multiple cell types	519:537	multiple cell types	519:537	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	5	42	from	present	508:514	arg1	species					553:559	mammalian species	543:559	mammalian species	543:559	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	7	43	theme	anti-dsRNA	847:856	arg1	antibodies					858:867	anti-dsRNA antibodies	847:867	anti-dsRNA antibodies	847:867	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	4	44	theme	chemical	378:385	arg1	approaches					403:412	chemical and biochemical approaches	378:412	chemical and biochemical approaches	378:412	Using a battery of chemical and biochemical approaches, we found that conserved small noncoding RNAs bear sialylated glycans.					
34004145	4	45	gly	sialylated	465:474	arg1	glycans					476:482	sialylated glycans	465:482	sialylated glycans	465:482	Using a battery of chemical and biochemical approaches, we found that conserved small noncoding RNAs bear sialylated glycans.					
34004145	5	46	from	species	553:559	arg1	present					508:514	present	508:514	present	508:514	These "glycoRNAs" were present in multiple cell types and mammalian species, in cultured cells, and in vivo.					
34004145	7	47	theme	glycoRNAs	782:790	arg1	majority					770:777	the majority	766:777	the majority of glycoRNAs	766:790	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	7	47	theme	glycoRNAs	782:790	arg1	present					797:803	present	797:803	present	797:803	Analysis of living cells revealed that the majority of glycoRNAs were present on the cell surface and can interact with anti-dsRNA antibodies and members of the Siglec receptor family.					
34004145	8	48	theme	extracellular	1055:1067	arg1	biology					1069:1075	extracellular biology	1055:1075	extracellular biology	1055:1075	Collectively, these findings suggest the existence of a direct interface between RNA biology and glycobiology, and an expanded role for RNA in extracellular biology.					
34004145	8	49	from	existence	953:961	arg1	biology					1069:1075	extracellular biology	1055:1075	extracellular biology	1055:1075	Collectively, these findings suggest the existence of a direct interface between RNA biology and glycobiology, and an expanded role for RNA in extracellular biology.					
34004145	4	50	theme	noncoding	445:453	arg1	RNAs					455:458	conserved small noncoding RNAs	429:458	conserved small noncoding RNAs	429:458	Using a battery of chemical and biochemical approaches, we found that conserved small noncoding RNAs bear sialylated glycans.					
34004145	4	51	contain	bear	460:463	arg1	RNAs					455:458	conserved small noncoding RNAs	429:458	conserved small noncoding RNAs	429:458	Using a battery of chemical and biochemical approaches, we found that conserved small noncoding RNAs bear sialylated glycans.					
34004145	4	51	contain	bear	460:463	arg2	glycans					476:482	sialylated glycans	465:482	sialylated glycans	465:482	Using a battery of chemical and biochemical approaches, we found that conserved small noncoding RNAs bear sialylated glycans.					
34004145	0	52	mod	modified	15:22	arg1	RNAs					6:9	Small RNAs	0:9	Small RNAs	0:9	Small RNAs are modified with N-glycans and displayed on the surface of living cells.					
34004145	0	52	mod	modified	15:22	arg3	N-glycans					29:37	N-glycans	29:37	N-glycans	29:37	Small RNAs are modified with N-glycans and displayed on the surface of living cells.					
33439436	6	0	dep	molecules	1319:1327	arg1	both					1302:1305	both	1302:1305	both	1302:1305	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	4	1	theme	N-terminal	901:910	arg1	domain					912:917	the N-terminal domain	897:917	the N-terminal domain	897:917	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	3	2	theme	N-terminal	579:588	arg1	EGREGEQ-					614:621	EGREGEQ-	614:621	EGREGEQ-	614:621	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	3	2	theme	N-terminal	579:588	arg1	sequence					601:608	the N-terminal amino acid sequence	575:608	the N-terminal amino acid sequence	575:608	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	8	3	theme	Ara	1812:1814	arg1	h					1816:1816	Ara h 1	1812:1818	Ara h 1	1812:1818	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	4	4	theme	new	703:705	arg1	isoform					707:713	This new isoform	698:713	This new isoform	698:713	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	4	5	theme	interaction	773:783	arg1	chromatography					785:798	hydrophobic interaction chromatography	761:798	hydrophobic interaction chromatography	761:798	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	8	6	theme	h	1974:1974	arg1	IgE					1988:1990	the Ara h 1- specific IgE	1966:1990	the Ara h 1- specific IgE in the patient	1966:2005	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	3	7	theme	acid	596:599	arg1	EGREGEQ-					614:621	EGREGEQ-	614:621	EGREGEQ-	614:621	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	3	7	theme	acid	596:599	arg1	sequence					601:608	the N-terminal amino acid sequence	575:608	the N-terminal amino acid sequence	575:608	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	0	8	theme	oligomer	98:105	arg1	structure					107:115	oligomer structure	98:115	oligomer structure	98:115	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	6	9	from	patients	1275:1282	arg1	antisera					1245:1252	antisera	1245:1252	antisera from peanut-allergic patients	1245:1282	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	6	10	theme	immunoglobulin	1334:1347	arg1	E					1349:1349	immunoglobulin E	1334:1349	the immunoglobulin E (IgE) antibodies in these sera	1330:1380	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	6	10	theme	immunoglobulin	1334:1347	arg1	IgE					1352:1354	IgE	1352:1354	IgE	1352:1354	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	2	11	theme	clone	458:462	arg1	41B					464:466	clone 41B	458:466	clone 41B	458:466	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	2	11	theme	clone	458:462	arg1	genes					451:455	two homologous genes	436:455	two homologous genes (clone 41B and clone P17)	436:481	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	6	12	theme	h	1490:1490	arg1	C-terminal					1438:1447	C-terminal	1438:1447	C-terminal	1438:1447	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	8	13	theme	specific	1979:1986	arg1	IgE					1988:1990	the Ara h 1- specific IgE	1966:1990	the Ara h 1- specific IgE in the patient	1966:2005	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	3	14	from	54 kDa	558:563	arg1	EGREGEQ-					614:621	EGREGEQ-	614:621	EGREGEQ-	614:621	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	3	14	from	54 kDa	558:563	arg1	sequence					601:608	the N-terminal amino acid sequence	575:608	the N-terminal amino acid sequence	575:608	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	1	15	theme	food	190:193	arg1	allergies					195:203	the most severe food allergies	174:203	the most severe food allergies	174:203	Peanut allergies are among the most severe food allergies, and several allergenic proteins referred to as Ara h 1-Ara h 17 have been identified from peanut seeds.					
33439436	4	16	theme	run-through	737:747	arg1	fraction					749:756	the run-through fraction	733:756	the run-through fraction of hydrophobic interaction chromatography	733:798	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	1	17	theme	Ara	253:255	arg1	h					265:265	Ara h 1-Ara h 17	253:268	Ara h 1-Ara h 17	253:268	Peanut allergies are among the most severe food allergies, and several allergenic proteins referred to as Ara h 1-Ara h 17 have been identified from peanut seeds.					
33439436	6	18	theme	Ara	1400:1402	arg1	molecule					1408:1415	each Ara h 1 molecule	1395:1415	each Ara h 1 molecule	1395:1415	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	6	19	theme	N-terminal	1464:1473	arg1	domains					1475:1481	the N-terminal domains	1460:1481	the C-terminal as well as the N-terminal domains of Ara h 1	1434:1492	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	8	20	from	cross-reactivity	1780:1795	arg1	serum					1827:1831	the serum	1823:1831	the serum of one peanut allergy patient	1823:1861	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	3	21	theme	N-terminal	644:653	arg1	1 had					678:682	63 kDa Ara h 1 had	665:682	63 kDa Ara h 1 had	665:682	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	3	21	theme	N-terminal	644:653	arg1	domain					655:660	the N-terminal domain	640:660	the N-terminal domain of 63 kDa Ara h 1 had	640:682	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	0	22	theme	h	68:68	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	0	22	theme	h	68:68	arg1	characterization					27:42	molecular characterization	17:42	molecular characterization	17:42	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	0	22	theme	h	68:68	arg1	allergen					88:95	a major peanut allergen: oligomer structure, antigenicity, and glycoform	73:144	a major peanut allergen: oligomer structure, antigenicity, and glycoform	73:144	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	0	23	theme	molecular	17:25	arg1	characterization					27:42	molecular characterization	17:42	molecular characterization	17:42	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	7	24	attach	linked	1751:1756	arg2	N-glycans					1737:1745	both typical high-mannose type and β-xylosylated type N-glycans	1683:1745	both typical high-mannose type and β-xylosylated type N-glycans	1683:1745	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	7	24	attach	linked	1751:1756	arg1	molecules					1765:1773	the molecules	1761:1773	the molecules	1761:1773	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	3	25	theme	1 had	678:682	arg1	1 had					678:682	63 kDa Ara h 1 had	665:682	63 kDa Ara h 1 had	665:682	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	3	25	theme	1 had	678:682	arg1	domain					655:660	the N-terminal domain	640:660	the N-terminal domain of 63 kDa Ara h 1 had	640:682	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	1	26	theme	peanut	296:301	arg1	seeds					303:307	peanut seeds	296:307	peanut seeds	296:307	Peanut allergies are among the most severe food allergies, and several allergenic proteins referred to as Ara h 1-Ara h 17 have been identified from peanut seeds.					
33439436	4	27	theme	Ara	813:815	arg1	h					817:817	63 kDa Ara h 1	806:819	63 kDa Ara h 1	806:819	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	6	28	theme	epitope	1526:1532	arg1	formations					1534:1543	the epitope formations	1522:1543	the epitope formations of this peanut glycoallergen	1522:1572	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	3	29	theme	Ara	672:674	arg1	1 had					678:682	63 kDa Ara h 1 had	665:682	63 kDa Ara h 1 had	665:682	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	0	30	theme	major	75:79	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	0	30	theme	major	75:79	arg1	allergen					88:95	a major peanut allergen: oligomer structure, antigenicity, and glycoform	73:144	a major peanut allergen: oligomer structure, antigenicity, and glycoform	73:144	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	0	31	dep	allergen	88:95	arg1	antigenicity					118:129	antigenicity	118:129	antigenicity	118:129	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	0	31	dep	allergen	88:95	arg1	glycoform					136:144	glycoform	136:144	glycoform	136:144	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	0	31	dep	allergen	88:95	arg1	structure					107:115	oligomer structure	98:115	oligomer structure	98:115	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	8	32	theme	IgE	1800:1802	arg1	cross-reactivity					1780:1795	The cross-reactivity	1776:1795	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient	1776:1861	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	7	33	theme	type	1732:1735	arg1	N-glycans					1737:1745	both typical high-mannose type and β-xylosylated type N-glycans	1683:1745	both typical high-mannose type and β-xylosylated type N-glycans	1683:1745	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	6	34	from	antibodies	1357:1366	arg1	sera					1377:1380	these sera	1371:1380	these sera	1371:1380	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	8	35	theme	peanut	1840:1845	arg1	patient					1855:1861	one peanut allergy patient	1836:1861	one peanut allergy patient	1836:1861	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	5	36	theme	63 kDa	1175:1180	arg1	molecule					1182:1189	the 63 kDa molecule	1171:1189	the 63 kDa molecule	1171:1189	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	2	37	theme	Ara	344:346	arg1	allergen					377:384	a glycosylated allergen	362:384	a glycosylated allergen	362:384	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	2	37	theme	Ara	344:346	arg1	63 kDa					353:358	Ara h 1 (63 kDa)	344:359	Ara h 1 (63 kDa)	344:359	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	3	38	dep	54 kDa	558:563	arg1	i.e.					553:556	i.e.	553:556	i.e.	553:556	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	8	39	theme	patient	1855:1861	arg1	serum					1827:1831	the serum	1823:1831	the serum of one peanut allergy patient	1823:1861	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	2	40	theme	glycosylated	364:375	arg1	allergen					377:384	a glycosylated allergen	362:384	a glycosylated allergen	362:384	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	2	40	theme	glycosylated	364:375	arg1	63 kDa					353:358	Ara h 1 (63 kDa)	344:359	Ara h 1 (63 kDa)	344:359	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	6	41	theme	peanut-allergic	1259:1273	arg1	patients					1275:1282	peanut-allergic patients	1259:1282	peanut-allergic patients	1259:1282	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	1	42	theme	h	257:257	arg1	h					265:265	Ara h 1-Ara h 17	253:268	Ara h 1-Ara h 17	253:268	Peanut allergies are among the most severe food allergies, and several allergenic proteins referred to as Ara h 1-Ara h 17 have been identified from peanut seeds.					
33439436	7	43	theme	high-mannose	1696:1707	arg1	type					1709:1712	typical high-mannose type	1688:1712	typical high-mannose type	1688:1712	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	4	44	theme	hydrophobic	846:856	arg1	resins					858:863	the hydrophobic resins	842:863	the hydrophobic resins	842:863	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	5	45	theme	N-terminal	1150:1159	arg1	molecule					1182:1189	the 63 kDa molecule	1171:1189	the 63 kDa molecule	1171:1189	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	5	45	theme	N-terminal	1150:1159	arg1	domain					1161:1166	the N-terminal domain	1146:1166	the N-terminal domain of the 63 kDa molecule	1146:1189	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	2	46	theme	63 kDa	353:358	arg1	characterization					324:339	The molecular characterization	310:339	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen,	310:385	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	7	47	theme	54 kDa	1645:1650	arg1	subunits					1660:1667	63 kDa and 54 kDa Ara h 1 subunits	1634:1667	63 kDa and 54 kDa Ara h 1 subunits	1634:1667	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	5	48	theme	higher	1210:1215	arg1	oligomerization					1223:1237	higher order oligomerization	1210:1237	higher order oligomerization	1210:1237	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	0	49	theme	truncated-type	49:62	arg1	h					68:68	a truncated-type Ara h 1	47:70	a truncated-type Ara h 1	47:70	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	7	50	theme	63 kDa	1634:1639	arg1	subunits					1660:1667	63 kDa and 54 kDa Ara h 1 subunits	1634:1667	63 kDa and 54 kDa Ara h 1 subunits	1634:1667	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	6	51	theme	peanut	1553:1558	arg1	glycoallergen					1560:1572	this peanut glycoallergen	1548:1572	this peanut glycoallergen	1548:1572	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	4	52	theme	hydrophilic	939:949	arg1	properties					951:960	extreme hydrophilic properties	931:960	extreme hydrophilic properties	931:960	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	3	53	theme	Ara	545:547	arg1	h					549:549	Ara h 1	545:551	Ara h 1	545:551	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	6	54	theme	h	1315:1315	arg1	molecules					1319:1327	both the Ara h 1 molecules	1302:1327	molecules	1319:1327	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	5	55	theme	54 kDa	1079:1084	arg1	h					1090:1090	54 kDa Ara h 1	1079:1092	54 kDa Ara h 1	1079:1092	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	2	56	gly	glycosylated	364:375	arg1	allergen					377:384	a glycosylated allergen	362:384	a glycosylated allergen	362:384	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	2	56	gly	glycosylated	364:375	arg1	63 kDa					353:358	Ara h 1 (63 kDa)	344:359	Ara h 1 (63 kDa)	344:359	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	1	57	theme	allergenic	218:227	arg1	proteins					229:236	several allergenic proteins	210:236	several allergenic proteins referred to as Ara h 1-Ara h 17	210:268	Peanut allergies are among the most severe food allergies, and several allergenic proteins referred to as Ara h 1-Ara h 17 have been identified from peanut seeds.					
33439436	7	58	theme	glycoform	1592:1600	arg1	analyses					1602:1609	the glycoform analyses	1588:1609	the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits	1588:1667	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	8	59	theme	h	1937:1937	arg1	epitope					1954:1960	the sole epitope	1945:1960	the sole epitope for the Ara h 1- specific IgE in the patient	1945:2005	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	8	59	theme	h	1937:1937	arg1	N-glycan					1921:1928	the N-glycan	1917:1928	the N-glycan of Ara h 1	1917:1939	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	4	60	theme	domain	912:917	arg1	removal					886:892	the removal	882:892	the removal of the N-terminal domain	882:917	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	3	61	theme	amino	590:594	arg1	EGREGEQ-					614:621	EGREGEQ-	614:621	EGREGEQ-	614:621	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	3	61	theme	amino	590:594	arg1	sequence					601:608	the N-terminal amino acid sequence	575:608	the N-terminal amino acid sequence	575:608	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	1	62	theme	Peanut	147:152	arg1	allergies					154:162	Peanut allergies	147:162	Peanut allergies	147:162	Peanut allergies are among the most severe food allergies, and several allergenic proteins referred to as Ara h 1-Ara h 17 have been identified from peanut seeds.					
33439436	8	63	theme	sole	1949:1952	arg1	epitope					1954:1960	the sole epitope	1945:1960	the sole epitope for the Ara h 1- specific IgE in the patient	1945:2005	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	8	63	theme	sole	1949:1952	arg1	N-glycan					1921:1928	the N-glycan	1917:1928	the N-glycan of Ara h 1	1917:1939	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	5	64	theme	Ara	984:986	arg1	h					988:988	63 kDa Ara h 1	977:990	63 kDa Ara h 1	977:990	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	6	65	theme	E	1349:1349	arg1	antibodies					1357:1366	the immunoglobulin E (IgE) antibodies	1330:1366	the immunoglobulin E (IgE) antibodies in these sera	1330:1380	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	4	66	theme	hydrophobic	761:771	arg1	chromatography					785:798	hydrophobic interaction chromatography	761:798	hydrophobic interaction chromatography	761:798	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	8	67	theme	Ara	1970:1972	arg1	IgE					1988:1990	the Ara h 1- specific IgE	1966:1990	the Ara h 1- specific IgE in the patient	1966:2005	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	1	68	theme	severe	183:188	arg1	allergies					195:203	the most severe food allergies	174:203	the most severe food allergies	174:203	Peanut allergies are among the most severe food allergies, and several allergenic proteins referred to as Ara h 1-Ara h 17 have been identified from peanut seeds.					
33439436	4	69	theme	chromatography	785:798	arg1	fraction					749:756	the run-through fraction	733:756	the run-through fraction of hydrophobic interaction chromatography	733:798	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	6	70	theme	Ara	1486:1488	arg1	h					1490:1490	Ara h 1	1486:1492	Ara h 1	1486:1492	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	2	71	theme	genes	451:455	arg1	occurrence					422:431	the occurrence	418:431	the occurrence of two homologous genes (clone 41B and clone P17)	418:481	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	6	72	theme	h	1404:1404	arg1	molecule					1408:1415	each Ara h 1 molecule	1395:1415	each Ara h 1 molecule	1395:1415	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	2	73	theme	clone	472:476	arg1	P17					478:480	clone P17	472:480	clone P17	472:480	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	2	73	theme	clone	472:476	arg1	genes					451:455	two homologous genes	436:455	two homologous genes (clone 41B and clone P17)	436:481	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	3	74	theme	63 kDa	665:670	arg1	1 had					678:682	63 kDa Ara h 1 had	665:682	63 kDa Ara h 1 had	665:682	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	4	75	theme	63 kDa	806:811	arg1	h					817:817	63 kDa Ara h 1	806:819	63 kDa Ara h 1	806:819	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	5	76	theme	63 kDa	977:982	arg1	h					988:988	63 kDa Ara h 1	977:990	63 kDa Ara h 1	977:990	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	5	77	dep	order	1009:1013	arg1	conformations					1031:1043	homo-oligomeric conformations	1015:1043	higher order homo-oligomeric conformations such as decamer or nonamer	1002:1070	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	0	78	theme	peanut	81:86	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	0	78	theme	peanut	81:86	arg1	allergen					88:95	a major peanut allergen: oligomer structure, antigenicity, and glycoform	73:144	a major peanut allergen: oligomer structure, antigenicity, and glycoform	73:144	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	2	79	theme	homologous	440:449	arg1	41B					464:466	clone 41B	458:466	clone 41B	458:466	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	2	79	theme	homologous	440:449	arg1	P17					478:480	clone P17	472:480	clone P17	472:480	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	2	79	theme	homologous	440:449	arg1	genes					451:455	two homologous genes	436:455	two homologous genes (clone 41B and clone P17)	436:481	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	3	80	theme	h	676:676	arg1	1 had					678:682	63 kDa Ara h 1 had	665:682	63 kDa Ara h 1 had	665:682	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	7	81	theme	β-xylosylated	1718:1730	arg1	type					1732:1735	β-xylosylated type	1718:1735	β-xylosylated type	1718:1735	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	8	82	from	IgE	1988:1990	arg1	patient					1999:2005	the patient	1995:2005	the patient	1995:2005	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	8	83	theme	allergy	1847:1853	arg1	patient					1855:1861	one peanut allergy patient	1836:1861	one peanut allergy patient	1836:1861	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	2	84	theme	h	348:348	arg1	allergen					377:384	a glycosylated allergen	362:384	a glycosylated allergen	362:384	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	2	84	theme	h	348:348	arg1	63 kDa					353:358	Ara h 1 (63 kDa)	344:359	Ara h 1 (63 kDa)	344:359	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	7	85	theme	type	1709:1712	arg1	N-glycans					1737:1745	both typical high-mannose type and β-xylosylated type N-glycans	1683:1745	both typical high-mannose type and β-xylosylated type N-glycans	1683:1745	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	3	86	from	EGREGEQ-	614:621	arg1	54 kDa					558:563	54 kDa	558:563	54 kDa	558:563	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	5	87	theme	homo-oligomeric	1015:1029	arg1	conformations					1031:1043	homo-oligomeric conformations	1015:1043	higher order homo-oligomeric conformations such as decamer or nonamer	1002:1070	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	7	88	theme	typical	1688:1694	arg1	type					1709:1712	typical high-mannose type	1688:1712	typical high-mannose type	1688:1712	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	7	89	theme	N-glycans	1614:1622	arg1	analyses					1602:1609	the glycoform analyses	1588:1609	the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits	1588:1667	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	3	90	theme	new	530:532	arg1	variant					534:540	a new variant	528:540	a new variant of Ara h 1	528:551	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	1	91	theme	1-Ara	259:263	arg1	h					265:265	Ara h 1-Ara h 17	253:268	Ara h 1-Ara h 17	253:268	Peanut allergies are among the most severe food allergies, and several allergenic proteins referred to as Ara h 1-Ara h 17 have been identified from peanut seeds.					
33439436	2	92	dep	genes	451:455	arg1	41B					464:466	clone 41B	458:466	clone 41B	458:466	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	2	92	dep	genes	451:455	arg1	P17					478:480	clone P17	472:480	clone P17	472:480	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	2	92	dep	genes	451:455	arg1	genes					451:455	two homologous genes	436:455	two homologous genes (clone 41B and clone P17)	436:481	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	0	93	theme	Ara	64:66	arg1	h					68:68	a truncated-type Ara h 1	47:70	a truncated-type Ara h 1	47:70	Purification and molecular characterization of a truncated-type Ara h 1, a major peanut allergen: oligomer structure, antigenicity, and glycoform.					
33439436	7	94	theme	Ara	1652:1654	arg1	subunits					1660:1667	63 kDa and 54 kDa Ara h 1 subunits	1634:1667	63 kDa and 54 kDa Ara h 1 subunits	1634:1667	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	6	95	theme	glycoallergen	1560:1572	arg1	formations					1534:1543	the epitope formations	1522:1543	the epitope formations of this peanut glycoallergen	1522:1572	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	3	96	theme	h	549:549	arg1	variant					534:540	a new variant	528:540	a new variant of Ara h 1	528:551	In this study, we found a new variant of Ara h 1 i.e. 54 kDa, in which the N-terminal amino acid sequence was EGREGEQ-, indicating that the N-terminal domain of 63 kDa Ara h 1 had been removed.					
33439436	5	97	theme	order	1217:1221	arg1	oligomerization					1223:1237	higher order oligomerization	1210:1237	higher order oligomerization	1210:1237	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	5	98	theme	higher	1002:1007	arg1	order					1009:1013	higher order	1002:1013	higher order homo-oligomeric conformations such as decamer or nonamer	1002:1070	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	7	99	theme	h	1656:1656	arg1	subunits					1660:1667	63 kDa and 54 kDa Ara h 1 subunits	1634:1667	63 kDa and 54 kDa Ara h 1 subunits	1634:1667	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	5	100	theme	molecule	1182:1189	arg1	molecule					1182:1189	the 63 kDa molecule	1171:1189	the 63 kDa molecule	1171:1189	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	5	100	theme	molecule	1182:1189	arg1	domain					1161:1166	the N-terminal domain	1146:1166	the N-terminal domain of the 63 kDa molecule	1146:1189	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
33439436	8	101	theme	Ara	1933:1935	arg1	h					1937:1937	Ara h 1	1933:1939	Ara h 1	1933:1939	The cross-reactivity of IgE against Ara h 1 in the serum of one peanut allergy patient was completely lost by de-N-glycosylation, indicating the N-glycan of Ara h 1 was the sole epitope for the Ara h 1- specific IgE in the patient.					
33439436	4	102	theme	extreme	931:937	arg1	properties					951:960	extreme hydrophilic properties	931:960	extreme hydrophilic properties	931:960	This new isoform was obtained from the run-through fraction of hydrophobic interaction chromatography while 63 kDa Ara h 1 was tightly bound to the hydrophobic resins, suggesting that the removal of the N-terminal domain resulted in extreme hydrophilic properties.					
33439436	7	103	attach	linked	1624:1629	arg1	subunits					1660:1667	63 kDa and 54 kDa Ara h 1 subunits	1634:1667	63 kDa and 54 kDa Ara h 1 subunits	1634:1667	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	7	103	attach	linked	1624:1629	arg2	N-glycans					1614:1622	N-glycans	1614:1622	N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits	1614:1667	Furthermore, the glycoform analyses of N-glycans linked to 63 kDa and 54 kDa Ara h 1 subunits revealed that both typical high-mannose type and β-xylosylated type N-glycans are linked to the molecules.					
33439436	1	104	theme	several	210:216	arg1	proteins					229:236	several allergenic proteins	210:236	several allergenic proteins referred to as Ara h 1-Ara h 17	210:268	Peanut allergies are among the most severe food allergies, and several allergenic proteins referred to as Ara h 1-Ara h 17 have been identified from peanut seeds.					
33439436	2	105	theme	molecular	314:322	arg1	characterization					324:339	The molecular characterization	310:339	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen,	310:385	The molecular characterization of Ara h 1 (63 kDa), a glycosylated allergen, has almost been completed, and the occurrence of two homologous genes (clone 41B and clone P17) has been identified.					
33439436	6	106	theme	Ara	1311:1313	arg1	molecules					1319:1327	both the Ara h 1 molecules	1302:1327	molecules	1319:1327	When antisera from peanut-allergic patients were treated with both the Ara h 1 molecules, the immunoglobulin E (IgE) antibodies in these sera reacted with each Ara h 1 molecule, suggesting that the C-terminal as well as the N-terminal domains of Ara h 1 contribute significantly to the epitope formations of this peanut glycoallergen.					
33439436	5	107	theme	Ara	1086:1088	arg1	h					1090:1090	54 kDa Ara h 1	1079:1092	54 kDa Ara h 1	1079:1092	We found that 63 kDa Ara h 1 occurs as higher order homo-oligomeric conformations such as decamer or nonamer, while 54 kDa Ara h 1 occurs exclusively as a homotrimer, indicating that the N-terminal domain of the 63 kDa molecule may be involved in higher order oligomerization.					
34698497	0	0	theme	Lipid	86:90	arg1	Rafts					92:96	Lipid Rafts	86:96	Lipid Rafts	86:96	Hierarchical Fluorescence Imaging Strategy for Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane.					
34698497	7	1	theme	raft-carried	1478:1489	arg1	techniques					1524:1533	raft-carried glycoconjugate-based theranostic techniques	1478:1533	raft-carried glycoconjugate-based theranostic techniques	1478:1533	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	3	2	theme	raft	525:528	arg1	glycans					548:554	raft and raft-harbored glycans	525:554	raft and raft-harbored glycans on the cell surface	525:574	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	6	3	from	variation	1233:1241	arg1	processes					1267:1275	different biological processes	1246:1275	different biological processes	1246:1275	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	3	4	theme	fluorescence	714:725	arg1	strategy					735:742	a hierarchical fluorescence imaging strategy	699:742	a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar	699:798	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	7	5	theme	glycoconjugate-based	1491:1510	arg1	techniques					1524:1533	raft-carried glycoconjugate-based theranostic techniques	1478:1533	raft-carried glycoconjugate-based theranostic techniques	1478:1533	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	5	6	from	probes	911:916	arg1	surface					930:936	the cell surface	921:936	the cell surface	921:936	After specifically anchoring the two probes on the cell surface, the raft probe can be cyclically utilized to turn on the fluorescence of the Sia probe, only residing in rafts, via a proximity cascade DNA reaction.					
34698497	6	7	theme	duplex	1093:1098	arg1	capability					1108:1117	The duplex imaging capability	1089:1117	The duplex imaging capability for spatially relevant levels of biological structures	1089:1172	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	3	8	theme	imaging	727:733	arg1	strategy					735:742	a hierarchical fluorescence imaging strategy	699:742	a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar	699:798	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	9	theme	raft-harbored	534:546	arg1	glycans					548:554	raft and raft-harbored glycans	525:554	raft and raft-harbored glycans on the cell surface	525:574	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	10	theme	cell	563:566	arg1	surface					568:574	the cell surface	559:574	the cell surface	559:574	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	11	from	surface	568:574	arg1	visualization					508:520	simultaneous visualization	495:520	simultaneous visualization of raft and raft-harbored glycans on the cell surface	495:574	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	0	12	theme	Rafts	92:96	arg1	Level					77:81	the Sialylation Level	61:81	the Sialylation Level of Lipid Rafts on the Cell Membrane	61:117	Hierarchical Fluorescence Imaging Strategy for Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane.					
34698497	5	13	theme	proximity	1057:1065	arg1	reaction					1079:1086	a proximity cascade DNA reaction	1055:1086	a proximity cascade DNA reaction	1055:1086	After specifically anchoring the two probes on the cell surface, the raft probe can be cyclically utilized to turn on the fluorescence of the Sia probe, only residing in rafts, via a proximity cascade DNA reaction.					
34698497	6	14	theme	biological	1256:1265	arg1	processes					1267:1275	different biological processes	1246:1275	different biological processes	1246:1275	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	6	15	theme	biological	1152:1161	arg1	structures					1163:1172	biological structures	1152:1172	biological structures	1152:1172	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	3	16	theme	simultaneous	495:506	arg1	visualization					508:520	simultaneous visualization	495:520	simultaneous visualization of raft and raft-harbored glycans on the cell surface	495:574	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	0	17	theme	Cell	105:108	arg1	Membrane					110:117	the Cell Membrane	101:117	the Cell Membrane	101:117	Hierarchical Fluorescence Imaging Strategy for Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane.					
34698497	5	18	theme	cascade	1067:1073	arg1	reaction					1079:1086	a proximity cascade DNA reaction	1055:1086	a proximity cascade DNA reaction	1055:1086	After specifically anchoring the two probes on the cell surface, the raft probe can be cyclically utilized to turn on the fluorescence of the Sia probe, only residing in rafts, via a proximity cascade DNA reaction.					
34698497	6	19	theme	different	1246:1254	arg1	processes					1267:1275	different biological processes	1246:1275	different biological processes	1246:1275	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	3	20	from	glycans	548:554	arg1	surface					568:574	the cell surface	559:574	the cell surface	559:574	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	2	21	theme	processes	295:303	arg1	revelation					222:231	The revelation	218:231	The revelation of glycosylation-mediated regulation mechanisms of biological processes	218:303	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	1	22	theme	ubiquitous	153:162	arg1	modifications					180:192	the most ubiquitous and complicated modifications	144:192	the most ubiquitous and complicated modifications of proteins and lipids	144:215	Glycosylation is one of the most ubiquitous and complicated modifications of proteins and lipids.					
34698497	0	23	from	Assessment	47:56	arg1	Membrane					110:117	the Cell Membrane	101:117	the Cell Membrane	101:117	Hierarchical Fluorescence Imaging Strategy for Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane.					
34698497	2	24	theme	biological	284:293	arg1	processes					295:303	biological processes	284:303	biological processes	284:303	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	7	25	from	interrogation	1336:1348	arg1	composition					1394:1404	raft composition	1389:1404	raft composition	1389:1404	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	7	25	from	interrogation	1336:1348	arg1	organization					1407:1418	organization	1407:1418	organization	1407:1418	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	7	25	from	interrogation	1336:1348	arg1	functions					1425:1433	functions	1425:1433	functions	1425:1433	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	7	26	theme	powerful	1318:1325	arg1	tool					1327:1330	an elegant and powerful tool	1303:1330	an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions	1303:1433	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	0	27	theme	Fluorescence	13:24	arg1	Imaging					26:32	Hierarchical Fluorescence Imaging	0:32	Hierarchical Fluorescence Imaging	0:32	Hierarchical Fluorescence Imaging Strategy for Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane.					
34698497	6	28	theme	relevant	1133:1140	arg1	levels					1142:1147	spatially relevant levels	1123:1147	spatially relevant levels of biological structures	1123:1172	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	1	29	theme	complicated	168:178	arg1	modifications					180:192	the most ubiquitous and complicated modifications	144:192	the most ubiquitous and complicated modifications of proteins and lipids	144:215	Glycosylation is one of the most ubiquitous and complicated modifications of proteins and lipids.					
34698497	0	30	theme	Hierarchical	0:11	arg1	Imaging					26:32	Hierarchical Fluorescence Imaging	0:32	Hierarchical Fluorescence Imaging	0:32	Hierarchical Fluorescence Imaging Strategy for Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane.					
34698497	6	31	theme	Sia	1229:1231	arg1	variation					1233:1241	raft-confined Sia variation	1215:1241	raft-confined Sia variation in different biological processes	1215:1275	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	3	32	from	visualization	508:520	arg1	surface					568:574	the cell surface	559:574	the cell surface	559:574	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	33	theme	specific	588:595	arg1	recognition					602:612	specific raft recognition	588:612	specific raft recognition	588:612	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	7	34	theme	techniques	1524:1533	arg1	development					1463:1473	the development	1459:1473	the development of raft-carried glycoconjugate-based theranostic techniques	1459:1533	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	1	35	theme	modifications	180:192	arg1	modifications					180:192	the most ubiquitous and complicated modifications	144:192	the most ubiquitous and complicated modifications of proteins and lipids	144:215	Glycosylation is one of the most ubiquitous and complicated modifications of proteins and lipids.					
34698497	1	35	theme	modifications	180:192	arg1	one					137:139	one	137:139	one	137:139	Glycosylation is one of the most ubiquitous and complicated modifications of proteins and lipids.					
34698497	2	36	theme	lipid	453:457	arg1	raft					459:462	lipid raft	453:462	lipid raft	453:462	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	3	37	theme	model	788:792	arg1	acid					770:773	N-acetyl-neuraminic acid	750:773	N-acetyl-neuraminic acid (Sia)	750:779	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	37	theme	model	788:792	arg1	sugar					794:798	the model sugar	784:798	the model sugar	784:798	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	5	38	theme	probe	1020:1024	arg1	fluorescence					996:1007	the fluorescence	992:1007	the fluorescence of the Sia probe, only residing in rafts	992:1048	After specifically anchoring the two probes on the cell surface, the raft probe can be cyclically utilized to turn on the fluorescence of the Sia probe, only residing in rafts, via a proximity cascade DNA reaction.					
34698497	6	39	theme	raft-confined	1215:1227	arg1	variation					1233:1241	raft-confined Sia variation	1215:1241	raft-confined Sia variation in different biological processes	1215:1275	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	7	40	theme	mechanisms	1375:1384	arg1	interrogation					1336:1348	interrogation	1336:1348	interrogation of the glycan regulation mechanisms on raft composition, organization, and functions	1336:1433	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	5	41	theme	raft	943:946	arg1	probe					948:952	the raft probe	939:952	the raft probe	939:952	After specifically anchoring the two probes on the cell surface, the raft probe can be cyclically utilized to turn on the fluorescence of the Sia probe, only residing in rafts, via a proximity cascade DNA reaction.					
34698497	6	42	theme	structures	1163:1172	arg1	levels					1142:1147	spatially relevant levels	1123:1147	spatially relevant levels of biological structures	1123:1172	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	3	43	theme	hybridization	663:675	arg1	techniques					677:686	specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques	588:686	specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques	588:686	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	5	44	theme	DNA	1075:1077	arg1	reaction					1079:1086	a proximity cascade DNA reaction	1055:1086	a proximity cascade DNA reaction	1055:1086	After specifically anchoring the two probes on the cell surface, the raft probe can be cyclically utilized to turn on the fluorescence of the Sia probe, only residing in rafts, via a proximity cascade DNA reaction.					
34698497	5	45	theme	Sia	1016:1018	arg1	probe					1020:1024	the Sia probe	1012:1024	the Sia probe	1012:1024	After specifically anchoring the two probes on the cell surface, the raft probe can be cyclically utilized to turn on the fluorescence of the Sia probe, only residing in rafts, via a proximity cascade DNA reaction.					
34698497	1	46	theme	proteins	197:204	arg1	modifications					180:192	the most ubiquitous and complicated modifications	144:192	the most ubiquitous and complicated modifications of proteins and lipids	144:215	Glycosylation is one of the most ubiquitous and complicated modifications of proteins and lipids.					
34698497	5	47	theme	cell	925:928	arg1	surface					930:936	the cell surface	921:936	the cell surface	921:936	After specifically anchoring the two probes on the cell surface, the raft probe can be cyclically utilized to turn on the fluorescence of the Sia probe, only residing in rafts, via a proximity cascade DNA reaction.					
34698497	2	48	gly	heterogeneity	365:377	arg1	glycans					395:401	cell surface glycans	382:401	cell surface glycans	382:401	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	5	49	used	utilized	972:979	arg2	probe					948:952	the raft probe	939:952	the raft probe	939:952	After specifically anchoring the two probes on the cell surface, the raft probe can be cyclically utilized to turn on the fluorescence of the Sia probe, only residing in rafts, via a proximity cascade DNA reaction.					
34698497	2	50	theme	glycans	395:401	arg1	heterogeneity					365:377	the spatial heterogeneity	353:377	the spatial heterogeneity of cell surface glycans	353:401	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	0	51	from	Level	77:81	arg1	Membrane					110:117	the Cell Membrane	101:117	the Cell Membrane	101:117	Hierarchical Fluorescence Imaging Strategy for Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane.					
34698497	3	52	theme	glycan	615:620	arg1	labeling					637:644	glycan chemoselective labeling	615:644	glycan chemoselective labeling	615:644	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	7	53	theme	glycan	1357:1362	arg1	mechanisms					1375:1384	the glycan regulation mechanisms	1353:1384	the glycan regulation mechanisms	1353:1384	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	1	54	theme	lipids	210:215	arg1	modifications					180:192	the most ubiquitous and complicated modifications	144:192	the most ubiquitous and complicated modifications of proteins and lipids	144:215	Glycosylation is one of the most ubiquitous and complicated modifications of proteins and lipids.					
34698497	3	55	theme	dynamic	655:661	arg1	hybridization					663:675	DNA dynamic hybridization	651:675	DNA dynamic hybridization	651:675	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	56	theme	raft	597:600	arg1	recognition					602:612	specific raft recognition	588:612	specific raft recognition	588:612	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	57	theme	N-acetyl-neuraminic	750:768	arg1	acid					770:773	N-acetyl-neuraminic acid	750:773	N-acetyl-neuraminic acid (Sia)	750:779	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	57	theme	N-acetyl-neuraminic	750:768	arg1	sugar					794:798	the model sugar	784:798	the model sugar	784:798	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	57	theme	N-acetyl-neuraminic	750:768	arg1	Sia					776:778	Sia	776:778	Sia	776:778	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	2	58	theme	spatial	357:363	arg1	heterogeneity					365:377	the spatial heterogeneity	353:377	the spatial heterogeneity of cell surface glycans	353:401	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	2	59	theme	nonraft	467:473	arg1	domains					475:481	nonraft domains	467:481	nonraft domains	467:481	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	7	60	theme	elegant	1306:1312	arg1	tool					1327:1330	an elegant and powerful tool	1303:1330	an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions	1303:1433	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	7	61	theme	regulation	1364:1373	arg1	mechanisms					1375:1384	the glycan regulation mechanisms	1353:1384	the glycan regulation mechanisms	1353:1384	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	3	62	theme	recognition	602:612	arg1	techniques					677:686	specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques	588:686	specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques	588:686	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	63	theme	DNA	651:653	arg1	hybridization					663:675	DNA dynamic hybridization	651:675	DNA dynamic hybridization	651:675	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	64	theme	labeling	637:644	arg1	techniques					677:686	specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques	588:686	specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques	588:686	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	3	65	theme	hierarchical	701:712	arg1	strategy					735:742	a hierarchical fluorescence imaging strategy	699:742	a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar	699:798	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	4	66	theme	raft	816:819	arg1	probe					821:825	a raft probe	814:825	a raft probe	814:825	We fabricate a raft probe and Sia probe for rafts and Sia, respectively.					
34698497	4	67	theme	Sia	831:833	arg1	probe					835:839	Sia probe	831:839	Sia probe	831:839	We fabricate a raft probe and Sia probe for rafts and Sia, respectively.					
34698497	3	68	theme	chemoselective	622:635	arg1	labeling					637:644	glycan chemoselective labeling	615:644	glycan chemoselective labeling	615:644	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	7	69	theme	raft	1389:1392	arg1	composition					1394:1404	raft composition	1389:1404	raft composition	1389:1404	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	6	70	theme	reason	1204:1209	arg1	revelation					1186:1195	the revelation	1182:1195	the revelation of the reason for raft-confined Sia variation in different biological processes	1182:1275	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	2	71	theme	mechanisms	270:279	arg1	revelation					222:231	The revelation	218:231	The revelation of glycosylation-mediated regulation mechanisms of biological processes	218:303	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	3	72	theme	glycans	548:554	arg1	visualization					508:520	simultaneous visualization	495:520	simultaneous visualization of raft and raft-harbored glycans on the cell surface	495:574	To achieve simultaneous visualization of raft and raft-harbored glycans on the cell surface, we combine specific raft recognition, glycan chemoselective labeling, and DNA dynamic hybridization techniques to develop a hierarchical fluorescence imaging strategy using N-acetyl-neuraminic acid (Sia) as the model sugar.					
34698497	6	73	theme	imaging	1100:1106	arg1	capability					1108:1117	The duplex imaging capability	1089:1117	The duplex imaging capability for spatially relevant levels of biological structures	1089:1172	The duplex imaging capability for spatially relevant levels of biological structures enables the revelation of the reason for raft-confined Sia variation in different biological processes.					
34698497	0	74	theme	Level	77:81	arg1	Assessment					47:56	Assessment	47:56	Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane	47:117	Hierarchical Fluorescence Imaging Strategy for Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane.					
34698497	7	75	theme	theranostic	1512:1522	arg1	techniques					1524:1533	raft-carried glycoconjugate-based theranostic techniques	1478:1533	raft-carried glycoconjugate-based theranostic techniques	1478:1533	Thus, this work provides an elegant and powerful tool for interrogation of the glycan regulation mechanisms on raft composition, organization, and functions and also contributes to the development of raft-carried glycoconjugate-based theranostic techniques.					
34698497	2	76	theme	regulation	259:268	arg1	mechanisms					270:279	glycosylation-mediated regulation mechanisms	236:279	glycosylation-mediated regulation mechanisms	236:279	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	2	77	theme	surface	387:393	arg1	glycans					395:401	cell surface glycans	382:401	cell surface glycans	382:401	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	0	78	theme	Sialylation	65:75	arg1	Level					77:81	the Sialylation Level	61:81	the Sialylation Level of Lipid Rafts on the Cell Membrane	61:117	Hierarchical Fluorescence Imaging Strategy for Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane.					
34698497	2	79	theme	glycosylation-mediated	236:257	arg1	mechanisms					270:279	glycosylation-mediated regulation mechanisms	236:279	glycosylation-mediated regulation mechanisms	236:279	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	2	80	theme	cell	382:385	arg1	glycans					395:401	cell surface glycans	382:401	cell surface glycans	382:401	The revelation of glycosylation-mediated regulation mechanisms of biological processes relies critically on the tools that can reflect the spatial heterogeneity of cell surface glycans, for example, distinguishing glycans exhibited in lipid raft or nonraft domains.					
34698497	0	81	from	Membrane	110:117	arg1	Assessment					47:56	Assessment	47:56	Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane	47:117	Hierarchical Fluorescence Imaging Strategy for Assessment of the Sialylation Level of Lipid Rafts on the Cell Membrane.					
32737362	0	0	theme	visceral	73:80	arg1	leishmaniasis					82:94	visceral leishmaniasis	73:94	visceral leishmaniasis	73:94	Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis.					
32737362	12	1	theme	glycans	1478:1484	arg1	analysis					1448:1455	the first detailed structural analysis	1418:1455	the first detailed structural analysis of sand fly salivary glycans	1418:1484	Our work presents the first detailed structural analysis of sand fly salivary glycans.					
32737362	12	2	dep	detailed	1428:1435	arg1	structural					1437:1446	structural	1437:1446	structural	1437:1446	Our work presents the first detailed structural analysis of sand fly salivary glycans.					
32737362	12	3	theme	sand	1460:1463	arg1	glycans					1478:1484	sand fly salivary glycans	1460:1484	sand fly salivary glycans	1460:1484	Our work presents the first detailed structural analysis of sand fly salivary glycans.					
32737362	4	4	theme	visceral	604:611	arg1	leishmaniasis					613:625	visceral leishmaniasis	604:625	visceral leishmaniasis	604:625	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	7	5	gly	glycoproteins	865:877	arg1	glycoproteins					865:877	several candidate glycoproteins	847:877	several candidate glycoproteins	847:877	SDS-PAGE coupled to LC-MS analysis of sand fly saliva, before and after enzymatic deglycosylation, revealed several candidate glycoproteins.					
32737362	5	6	theme	Lu	682:683	arg1	half					674:677	half	674:677	half of Lu	674:683	In silico predictions suggest half of Lu.					
32737362	4	7	theme	N-glycans	526:534	arg1	profile					515:521	the profile	511:521	the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas	511:641	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	8	8	theme	exoglycosidase	1087:1100	arg1	treatments					1102:1111	exoglycosidase treatments	1087:1111	exoglycosidase treatments	1087:1111	To determine the diversity of N-glycan structures in sand fly saliva, enzymatically released sugars were fluorescently tagged and analysed by HPLC, combined with highly sensitive LC-MS/MS, MALDI-TOF-MS, and exoglycosidase treatments.					
32737362	12	9	theme	detailed	1428:1435	arg1	analysis					1448:1455	the first detailed structural analysis	1418:1455	the first detailed structural analysis of sand fly salivary glycans	1418:1484	Our work presents the first detailed structural analysis of sand fly salivary glycans.					
32737362	0	10	theme	leishmaniasis	82:94	arg1	vector					63:68	vector	63:68	vector of visceral leishmaniasis	63:94	Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis.					
32737362	0	10	theme	leishmaniasis	82:94	arg1	N-glycome					27:35	the salivary N-glycome	14:35	the salivary N-glycome of Lutzomyia longipalpis	14:60	Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis.					
32737362	10	11	theme	longipalpis	1160:1170	arg1	saliva					1172:1177	longipalpis saliva	1160:1177	longipalpis saliva	1160:1177	longipalpis saliva mostly consists of oligomannose sugars, with Man5GlcNAc2 being the most abundant, and a few hybrid-type species.					
32737362	4	12	from	profile	515:521	arg1	glycoproteins					554:566	the salivary glycoproteins	541:566	the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas	541:641	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	12	13	theme	first	1422:1426	arg1	analysis					1448:1455	the first detailed structural analysis	1418:1455	the first detailed structural analysis of sand fly salivary glycans	1418:1484	Our work presents the first detailed structural analysis of sand fly salivary glycans.					
32737362	8	14	theme	structures	919:928	arg1	diversity					897:905	the diversity	893:905	the diversity of N-glycan structures in sand fly saliva	893:947	To determine the diversity of N-glycan structures in sand fly saliva, enzymatically released sugars were fluorescently tagged and analysed by HPLC, combined with highly sensitive LC-MS/MS, MALDI-TOF-MS, and exoglycosidase treatments.					
32737362	4	15	theme	leishmaniasis	613:625	arg1	longipalpis					581:591	Lutzomyia longipalpis	571:591	Lutzomyia longipalpis	571:591	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	4	15	theme	leishmaniasis	613:625	arg1	vector					594:599	vector	594:599	vector of visceral leishmaniasis	594:625	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	3	16	theme	salivary	358:365	arg1	proteins					367:374	Sand fly salivary proteins	349:374	Sand fly salivary proteins	349:374	Sand fly salivary proteins have been extensively studied, but the nature and biological roles of protein-linked glycans remain overlooked.					
32737362	11	17	theme	144 Da	1352:1357	arg1	144 Da					1352:1357	144 Da	1352:1357	144 Da	1352:1357	Interestingly, some glycans appear modified with a group of 144 Da, whose identity has yet to be confirmed.					
32737362	11	17	theme	144 Da	1352:1357	arg1	group					1343:1347	a group	1341:1347	a group	1341:1347	Interestingly, some glycans appear modified with a group of 144 Da, whose identity has yet to be confirmed.					
32737362	5	18	theme	In	644:645	arg1	predictions					654:664	In silico predictions	644:664	In silico predictions	644:664	In silico predictions suggest half of Lu.					
32737362	8	19	theme	N-glycan	910:917	arg1	structures					919:928	N-glycan structures	910:928	N-glycan structures	910:928	To determine the diversity of N-glycan structures in sand fly saliva, enzymatically released sugars were fluorescently tagged and analysed by HPLC, combined with highly sensitive LC-MS/MS, MALDI-TOF-MS, and exoglycosidase treatments.					
32737362	3	20	theme	protein-linked	446:459	arg1	glycans					461:467	protein-linked glycans	446:467	protein-linked glycans	446:467	Sand fly salivary proteins have been extensively studied, but the nature and biological roles of protein-linked glycans remain overlooked.					
32737362	7	21	theme	LC-MS	759:763	arg1	analysis					765:772	LC-MS analysis	759:772	LC-MS analysis of sand fly saliva, before and after enzymatic deglycosylation,	759:836	SDS-PAGE coupled to LC-MS analysis of sand fly saliva, before and after enzymatic deglycosylation, revealed several candidate glycoproteins.					
32737362	12	22	theme	salivary	1469:1476	arg1	glycans					1478:1484	sand fly salivary glycans	1460:1484	sand fly salivary glycans	1460:1484	Our work presents the first detailed structural analysis of sand fly salivary glycans.					
32737362	3	23	dep	nature	415:420	arg1	the					411:413	the	411:413	the	411:413	Sand fly salivary proteins have been extensively studied, but the nature and biological roles of protein-linked glycans remain overlooked.					
32737362	8	24	theme	sensitive	1049:1057	arg1	LC-MS/MS					1059:1066	highly sensitive LC-MS/MS	1042:1066	highly sensitive LC-MS/MS	1042:1066	To determine the diversity of N-glycan structures in sand fly saliva, enzymatically released sugars were fluorescently tagged and analysed by HPLC, combined with highly sensitive LC-MS/MS, MALDI-TOF-MS, and exoglycosidase treatments.					
32737362	8	25	theme	sand	933:936	arg1	saliva					942:947	sand fly saliva	933:947	sand fly saliva	933:947	To determine the diversity of N-glycan structures in sand fly saliva, enzymatically released sugars were fluorescently tagged and analysed by HPLC, combined with highly sensitive LC-MS/MS, MALDI-TOF-MS, and exoglycosidase treatments.					
32737362	3	26	theme	glycans	461:467	arg1	nature					415:420	nature	415:420	nature	415:420	Sand fly salivary proteins have been extensively studied, but the nature and biological roles of protein-linked glycans remain overlooked.					
32737362	3	26	theme	glycans	461:467	arg1	roles					437:441	biological roles	426:441	biological roles	426:441	Sand fly salivary proteins have been extensively studied, but the nature and biological roles of protein-linked glycans remain overlooked.					
32737362	11	27	dep	appear	1320:1325	arg1	modified					1327:1334	modified	1327:1334	appear modified with a group of 144 Da, whose identity has yet to be confirmed	1320:1397	Interestingly, some glycans appear modified with a group of 144 Da, whose identity has yet to be confirmed.					
32737362	12	28	theme	fly	1465:1467	arg1	glycans					1478:1484	sand fly salivary glycans	1460:1484	sand fly salivary glycans	1460:1484	Our work presents the first detailed structural analysis of sand fly salivary glycans.					
32737362	3	29	link	protein-linked	446:459	arg1	glycans					461:467	protein-linked glycans	446:467	protein-linked glycans	446:467	Sand fly salivary proteins have been extensively studied, but the nature and biological roles of protein-linked glycans remain overlooked.					
32737362	9	30	dep	found	1117:1121	arg1	composition					1141:1151	the N-glycan composition	1128:1151	the N-glycan composition of Lu	1128:1157	We found that the N-glycan composition of Lu.					
32737362	2	31	theme	immune	331:336	arg1	responses					338:346	the host's immune responses	320:346	the host's immune responses	320:346	Saliva has anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses.					
32737362	4	32	theme	longipalpis	581:591	arg1	glycoproteins					554:566	the salivary glycoproteins	541:566	the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas	541:641	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	8	33	from	diversity	897:905	arg1	saliva					942:947	sand fly saliva	933:947	sand fly saliva	933:947	To determine the diversity of N-glycan structures in sand fly saliva, enzymatically released sugars were fluorescently tagged and analysed by HPLC, combined with highly sensitive LC-MS/MS, MALDI-TOF-MS, and exoglycosidase treatments.					
32737362	1	34	theme	vertebrates	187:197	arg1	skin					179:182	the skin	175:182	the skin of vertebrates	175:197	During Leishmania transmission sand flies inoculate parasites and saliva into the skin of vertebrates.					
32737362	0	35	theme	salivary	18:25	arg1	vector					63:68	vector	63:68	vector of visceral leishmaniasis	63:94	Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis.					
32737362	0	35	theme	salivary	18:25	arg1	N-glycome					27:35	the salivary N-glycome	14:35	the salivary N-glycome of Lutzomyia longipalpis	14:60	Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis.					
32737362	7	36	theme	several	847:853	arg1	glycoproteins					865:877	several candidate glycoproteins	847:877	several candidate glycoproteins	847:877	SDS-PAGE coupled to LC-MS analysis of sand fly saliva, before and after enzymatic deglycosylation, revealed several candidate glycoproteins.					
32737362	8	37	theme	fly	938:940	arg1	saliva					942:947	sand fly saliva	933:947	sand fly saliva	933:947	To determine the diversity of N-glycan structures in sand fly saliva, enzymatically released sugars were fluorescently tagged and analysed by HPLC, combined with highly sensitive LC-MS/MS, MALDI-TOF-MS, and exoglycosidase treatments.					
32737362	1	38	theme	Leishmania	104:113	arg1	transmission					115:126	Leishmania transmission	104:126	Leishmania transmission	104:126	During Leishmania transmission sand flies inoculate parasites and saliva into the skin of vertebrates.					
32737362	7	39	theme	candidate	855:863	arg1	glycoproteins					865:877	several candidate glycoproteins	847:877	several candidate glycoproteins	847:877	SDS-PAGE coupled to LC-MS analysis of sand fly saliva, before and after enzymatic deglycosylation, revealed several candidate glycoproteins.					
32737362	6	40	theme	salivary	698:705	arg1	proteins					707:714	longipalpis salivary proteins	686:714	longipalpis salivary proteins	686:714	longipalpis salivary proteins may be N-glycosylated.					
32737362	2	41	contain	has	207:209	arg1	Saliva					200:205	Saliva	200:205	Saliva	200:205	Saliva has anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses.					
32737362	2	41	contain	has	207:209	arg2	activities					250:259	anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses	211:346	anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses	211:346	Saliva has anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses.					
32737362	3	42	theme	biological	426:435	arg1	roles					437:441	biological roles	426:441	biological roles	426:441	Sand fly salivary proteins have been extensively studied, but the nature and biological roles of protein-linked glycans remain overlooked.					
32737362	4	43	theme	salivary	545:552	arg1	glycoproteins					554:566	the salivary glycoproteins	541:566	the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas	541:641	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	4	44	from	glycoproteins	554:566	arg1	Americas					634:641	the Americas	630:641	the Americas	630:641	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	4	44	from	glycoproteins	554:566	arg1	N-glycans					526:534	N-glycans	526:534	N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas	526:641	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	4	44	from	glycoproteins	554:566	arg1	profile					515:521	the profile	511:521	the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas	511:641	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	0	45	theme	longipalpis	50:60	arg1	vector					63:68	vector	63:68	vector of visceral leishmaniasis	63:94	Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis.					
32737362	0	45	theme	longipalpis	50:60	arg1	N-glycome					27:35	the salivary N-glycome	14:35	the salivary N-glycome of Lutzomyia longipalpis	14:60	Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis.					
32737362	7	46	theme	enzymatic	811:819	arg1	deglycosylation					821:835	enzymatic deglycosylation	811:835	enzymatic deglycosylation	811:835	SDS-PAGE coupled to LC-MS analysis of sand fly saliva, before and after enzymatic deglycosylation, revealed several candidate glycoproteins.					
32737362	2	47	theme	anti-inflammatory	232:248	arg1	activities					250:259	anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses	211:346	anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses	211:346	Saliva has anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses.					
32737362	8	48	theme	released	964:971	arg1	sugars					973:978	enzymatically released sugars	950:978	enzymatically released sugars	950:978	To determine the diversity of N-glycan structures in sand fly saliva, enzymatically released sugars were fluorescently tagged and analysed by HPLC, combined with highly sensitive LC-MS/MS, MALDI-TOF-MS, and exoglycosidase treatments.					
32737362	0	49	theme	Lutzomyia	40:48	arg1	longipalpis					50:60	Lutzomyia longipalpis	40:60	Lutzomyia longipalpis	40:60	Insights into the salivary N-glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis.					
32737362	2	50	theme	anti-haemostatic	211:226	arg1	activities					250:259	anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses	211:346	anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses	211:346	Saliva has anti-haemostatic and anti-inflammatory activities that evolved to facilitate bloodfeeding, but also modulate the host's immune responses.					
32737362	1	51	theme	sand	128:131	arg1	flies					133:137	sand flies	128:137	sand flies	128:137	During Leishmania transmission sand flies inoculate parasites and saliva into the skin of vertebrates.					
32737362	6	52	theme	longipalpis	686:696	arg1	proteins					707:714	longipalpis salivary proteins	686:714	longipalpis salivary proteins	686:714	longipalpis salivary proteins may be N-glycosylated.					
32737362	10	53	theme	oligomannose	1198:1209	arg1	sugars					1211:1216	oligomannose sugars	1198:1216	oligomannose sugars	1198:1216	longipalpis saliva mostly consists of oligomannose sugars, with Man5GlcNAc2 being the most abundant, and a few hybrid-type species.					
32737362	5	54	dep	In	644:645	arg1	silico					647:652	silico	647:652	silico	647:652	In silico predictions suggest half of Lu.					
32737362	7	55	theme	saliva	786:791	arg1	analysis					765:772	LC-MS analysis	759:772	LC-MS analysis of sand fly saliva, before and after enzymatic deglycosylation,	759:836	SDS-PAGE coupled to LC-MS analysis of sand fly saliva, before and after enzymatic deglycosylation, revealed several candidate glycoproteins.					
32737362	6	56	gly	N-glycosylated	723:736	arg1	proteins					707:714	longipalpis salivary proteins	686:714	longipalpis salivary proteins	686:714	longipalpis salivary proteins may be N-glycosylated.					
32737362	9	57	theme	Lu	1156:1157	arg1	composition					1141:1151	the N-glycan composition	1128:1151	the N-glycan composition of Lu	1128:1157	We found that the N-glycan composition of Lu.					
32737362	10	58	theme	hybrid-type	1271:1281	arg1	species					1283:1289	and a few hybrid-type species	1261:1289	species	1283:1289	longipalpis saliva mostly consists of oligomannose sugars, with Man5GlcNAc2 being the most abundant, and a few hybrid-type species.					
32737362	9	59	theme	N-glycan	1132:1139	arg1	composition					1141:1151	the N-glycan composition	1128:1151	the N-glycan composition of Lu	1128:1157	We found that the N-glycan composition of Lu.					
32737362	3	60	theme	Sand	349:352	arg1	proteins					367:374	Sand fly salivary proteins	349:374	Sand fly salivary proteins	349:374	Sand fly salivary proteins have been extensively studied, but the nature and biological roles of protein-linked glycans remain overlooked.					
32737362	10	61	theme	few	1267:1269	arg1	species					1283:1289	and a few hybrid-type species	1261:1289	species	1283:1289	longipalpis saliva mostly consists of oligomannose sugars, with Man5GlcNAc2 being the most abundant, and a few hybrid-type species.					
32737362	7	62	theme	sand	777:780	arg1	saliva					786:791	sand fly saliva	777:791	sand fly saliva	777:791	SDS-PAGE coupled to LC-MS analysis of sand fly saliva, before and after enzymatic deglycosylation, revealed several candidate glycoproteins.					
32737362	3	63	theme	fly	354:356	arg1	proteins					367:374	Sand fly salivary proteins	349:374	Sand fly salivary proteins	349:374	Sand fly salivary proteins have been extensively studied, but the nature and biological roles of protein-linked glycans remain overlooked.					
32737362	4	64	gly	glycoproteins	554:566	arg1	glycoproteins					554:566	the salivary glycoproteins	541:566	the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas	541:641	Here, we characterised the profile of N-glycans from the salivary glycoproteins of Lutzomyia longipalpis, vector of visceral leishmaniasis in the Americas.					
32737362	7	65	theme	fly	782:784	arg1	saliva					786:791	sand fly saliva	777:791	sand fly saliva	777:791	SDS-PAGE coupled to LC-MS analysis of sand fly saliva, before and after enzymatic deglycosylation, revealed several candidate glycoproteins.					
33662421	3	0	dep	composed	400:407	arg1	residues					601:608	1→ residues	598:608	1→ residues	598:608	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	5	1	theme	low-density	820:830	arg1	lipoprotein					832:842	low-density lipoprotein	820:842	low-density lipoprotein receptor	820:851	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	4	2	theme	1→	668:669	arg1	residues					671:678	the →2,6)-α-D-Manp(1→ residues	649:678	the →2,6)-α-D-Manp(1→ residues	649:678	Furthermore, O-6 and O-2 positions of the →2,6)-α-D-Manp(1→ residues were substituted with methyl and β-D-Galp, respectively.					
33662421	1	3	theme	column	260:265	arg1	chromatography					267:280	column chromatography	260:280	column chromatography	260:280	One novel alkali-extracted polysaccharide, CM3-SII, was obtained from the fruiting body of C. militaris via column chromatography.					
33662421	5	4	theme	lipoprotein	832:842	arg1	receptor					844:851	low-density lipoprotein receptor	820:851	low-density lipoprotein receptor	820:851	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	3	5	from	positions	502:510	arg1	glycosyls					466:474	1→ glycosyls	463:474	1→ glycosyls	463:474	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	3	5	from	positions	502:510	arg1	branching					481:489	branching	481:489	branching	481:489	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	3	6	dep	-α-D-Manp	588:596	arg1	glycosyls					531:539	1→ glycosyls	528:539	1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6	528:586	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	0	7	theme	alkali-extracted	94:109	arg1	polysaccharide					111:124	the novel alkali-extracted polysaccharide	84:124	the novel alkali-extracted polysaccharide	84:124	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	5	8	theme	hepatoma	973:980	arg1	cells					987:991	hepatoma Huh7 cells	973:991	hepatoma Huh7 cells	973:991	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	5	9	dep	receptor	844:851	arg1	type					896:899	subtilisin/kexin type 9	879:901	subtilisin/kexin type 9 (PCSK9)	879:909	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	5	9	dep	receptor	844:851	arg1	PCSK9					904:908	PCSK9	904:908	PCSK9	904:908	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	0	10	theme	novel	88:92	arg1	polysaccharide					111:124	the novel alkali-extracted polysaccharide	84:124	the novel alkali-extracted polysaccharide	84:124	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	4	11	theme	-α-D-Manp	658:666	arg1	residues					671:678	the →2,6)-α-D-Manp(1→ residues	649:678	the →2,6)-α-D-Manp(1→ residues	649:678	Furthermore, O-6 and O-2 positions of the →2,6)-α-D-Manp(1→ residues were substituted with methyl and β-D-Galp, respectively.					
33662421	5	12	theme	convertase	868:877	arg1	expression					806:815	the intracellular protein expression	780:815	the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9)	780:909	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	7	13	theme	reported	1141:1148	arg1	polysaccharides					1167:1181	the previously reported alkali-extracted polysaccharides	1126:1181	the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris	1126:1229	Collectively, CM3-SII is different from the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris, and it may have potential application in hypolipidemia or as a pharmaceutical additive.					
33662421	2	14	dep	chemical	336:343	arg1	methods					363:369	methods	363:369	methods	363:369	Its structural characteristics were investigated via chemical and spectroscopic methods.					
33662421	4	15	theme	residues	671:678	arg1	positions					636:644	O-6 and O-2 positions	624:644	positions	636:644	Furthermore, O-6 and O-2 positions of the →2,6)-α-D-Manp(1→ residues were substituted with methyl and β-D-Galp, respectively.					
33662421	5	16	from	protein	960:966	arg1	cells					987:991	hepatoma Huh7 cells	973:991	hepatoma Huh7 cells	973:991	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	0	17	theme	polysaccharide	111:124	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	0	17	theme	polysaccharide	111:124	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	0	17	theme	polysaccharide	111:124	arg1	effect					74:79	PCSK9 secretion inhibitory effect	47:79	PCSK9 secretion inhibitory effect	47:79	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	7	18	contain	have	1243:1246	arg1	it					1236:1237	it	1236:1237	it	1236:1237	Collectively, CM3-SII is different from the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris, and it may have potential application in hypolipidemia or as a pharmaceutical additive.					
33662421	7	18	contain	have	1243:1246	arg2	application					1258:1268	potential application	1248:1268	potential application	1248:1268	Collectively, CM3-SII is different from the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris, and it may have potential application in hypolipidemia or as a pharmaceutical additive.					
33662421	2	19	theme	structural	287:296	arg1	characteristics					298:312	Its structural characteristics	283:312	Its structural characteristics	283:312	Its structural characteristics were investigated via chemical and spectroscopic methods.					
33662421	6	20	theme	PCSK9	1035:1039	arg1	secretion					1041:1049	PCSK9 secretion	1035:1049	PCSK9 secretion	1035:1049	Of note, CM3-SII significantly decreased PCSK9 secretion at the concentration of 200 μg/mL.					
33662421	5	21	theme	subtilisin/kexin	879:894	arg1	type					896:899	subtilisin/kexin type 9	879:901	subtilisin/kexin type 9 (PCSK9)	879:909	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	5	21	theme	subtilisin/kexin	879:894	arg1	PCSK9					904:908	PCSK9	904:908	PCSK9	904:908	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	5	22	theme	intracellular	784:796	arg1	expression					806:815	the intracellular protein expression	780:815	the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9)	780:909	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	3	23	theme	-β-D-Manp	415:423	arg1	1→					442:443	→4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp	412:461	1→	442:443	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	0	24	theme	PCSK9	47:51	arg1	effect					74:79	PCSK9 secretion inhibitory effect	47:79	PCSK9 secretion inhibitory effect	47:79	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	3	25	theme	-β-D-Manp	432:440	arg1	1→					442:443	→4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp	412:461	1→	442:443	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	5	26	theme	Huh7	982:985	arg1	cells					987:991	hepatoma Huh7 cells	973:991	hepatoma Huh7 cells	973:991	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	3	27	theme	→6	515:516	arg1	positions					502:510	the O-4 positions	494:510	the O-4 positions of →6	494:516	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	3	28	theme	→6	450:451	arg1	-α-D-Manp					453:461	→4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp	412:461	-α-D-Manp	453:461	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	0	29	theme	structural	14:23	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	0	30	theme	Cordyceps	131:139	arg1	militaris					141:149	Cordyceps militaris	131:149	Cordyceps militaris	131:149	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	4	31	theme	→2,6	653:656	arg1	residues					671:678	the →2,6)-α-D-Manp(1→ residues	649:678	the →2,6)-α-D-Manp(1→ residues	649:678	Furthermore, O-6 and O-2 positions of the →2,6)-α-D-Manp(1→ residues were substituted with methyl and β-D-Galp, respectively.					
33662421	7	32	theme	militaris	1221:1229	arg1	body					1210:1213	the fruiting body	1197:1213	the fruiting body of C. militaris	1197:1229	Collectively, CM3-SII is different from the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris, and it may have potential application in hypolipidemia or as a pharmaceutical additive.					
33662421	6	33	theme	200 μg/mL	1075:1083	arg1	concentration					1058:1070	the concentration	1054:1070	the concentration of 200 μg/mL	1054:1083	Of note, CM3-SII significantly decreased PCSK9 secretion at the concentration of 200 μg/mL.					
33662421	7	34	theme	potential	1248:1256	arg1	application					1258:1268	potential application	1248:1268	potential application	1248:1268	Collectively, CM3-SII is different from the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris, and it may have potential application in hypolipidemia or as a pharmaceutical additive.					
33662421	5	35	theme	protein	798:804	arg1	expression					806:815	the intracellular protein expression	780:815	the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9)	780:909	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	1	36	theme	fruiting	226:233	arg1	body					235:238	the fruiting body	222:238	the fruiting body of C. militaris	222:254	One novel alkali-extracted polysaccharide, CM3-SII, was obtained from the fruiting body of C. militaris via column chromatography.					
33662421	7	37	from	polysaccharides	1167:1181	arg1	different					1111:1119	different	1111:1119	different	1111:1119	Collectively, CM3-SII is different from the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris, and it may have potential application in hypolipidemia or as a pharmaceutical additive.					
33662421	0	38	from	militaris	141:149	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	0	38	from	militaris	141:149	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	0	38	from	militaris	141:149	arg1	effect					74:79	PCSK9 secretion inhibitory effect	47:79	PCSK9 secretion inhibitory effect	47:79	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	7	39	attach	isolated	1183:1190	arg1	body					1210:1213	the fruiting body	1197:1213	the fruiting body of C. militaris	1197:1229	Collectively, CM3-SII is different from the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris, and it may have potential application in hypolipidemia or as a pharmaceutical additive.					
33662421	7	39	attach	isolated	1183:1190	arg2	polysaccharides					1167:1181	the previously reported alkali-extracted polysaccharides	1126:1181	the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris	1126:1229	Collectively, CM3-SII is different from the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris, and it may have potential application in hypolipidemia or as a pharmaceutical additive.					
33662421	3	40	theme	CM3-SII	388:394	arg1	backbone					376:383	The backbone	372:383	The backbone of CM3-SII	372:394	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	0	41	theme	secretion	53:61	arg1	effect					74:79	PCSK9 secretion inhibitory effect	47:79	PCSK9 secretion inhibitory effect	47:79	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	3	42	theme	1→	528:529	arg1	glycosyls					531:539	1→ glycosyls	528:539	1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6	528:586	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	3	43	theme	1→	463:464	arg1	glycosyls					466:474	1→ glycosyls	463:474	1→ glycosyls	463:474	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	3	44	theme	1→	425:426	arg1	1→					442:443	→4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp	412:461	1→	442:443	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	7	45	theme	alkali-extracted	1150:1165	arg1	polysaccharides					1167:1181	the previously reported alkali-extracted polysaccharides	1126:1181	the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris	1126:1229	Collectively, CM3-SII is different from the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris, and it may have potential application in hypolipidemia or as a pharmaceutical additive.					
33662421	3	46	dep	1→	442:443	arg1	glycosyls					466:474	1→ glycosyls	463:474	1→ glycosyls	463:474	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	3	46	dep	1→	442:443	arg1	branching					481:489	branching	481:489	branching	481:489	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	3	46	dep	1→	442:443	arg1	→6					429:430	→4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp	412:461	→6	429:430	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	4	47	theme	O-6	624:626	arg1	positions					636:644	O-6 and O-2 positions	624:644	positions	636:644	Furthermore, O-6 and O-2 positions of the →2,6)-α-D-Manp(1→ residues were substituted with methyl and β-D-Galp, respectively.					
33662421	5	48	theme	sterol	926:931	arg1	protein					960:966	sterol regulatory element-binding protein 2	926:968	sterol regulatory element-binding protein 2 in hepatoma Huh7 cells	926:991	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	5	49	theme	proprotein	857:866	arg1	convertase					868:877	proprotein convertase	857:877	proprotein convertase	857:877	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	3	50	dep	linked-β-D-Galf	562:576	arg1	1→2					557:559	1→2	557:559	1→2	557:559	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	3	51	theme	O-4	498:500	arg1	positions					502:510	the O-4 positions	494:510	the O-4 positions of →6	494:516	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	5	52	theme	receptor	844:851	arg1	expression					806:815	the intracellular protein expression	780:815	the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9)	780:909	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	5	53	theme	regulatory	933:942	arg1	protein					960:966	sterol regulatory element-binding protein 2	926:968	sterol regulatory element-binding protein 2 in hepatoma Huh7 cells	926:991	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	4	54	theme	O-2	632:634	arg1	positions					636:644	O-6 and O-2 positions	624:644	positions	636:644	Furthermore, O-6 and O-2 positions of the →2,6)-α-D-Manp(1→ residues were substituted with methyl and β-D-Galp, respectively.					
33662421	7	55	theme	fruiting	1201:1208	arg1	body					1210:1213	the fruiting body	1197:1213	the fruiting body of C. militaris	1197:1229	Collectively, CM3-SII is different from the previously reported alkali-extracted polysaccharides isolated from the fruiting body of C. militaris, and it may have potential application in hypolipidemia or as a pharmaceutical additive.					
33662421	3	56	theme	-α-D-Manp	588:596	arg1	1→					442:443	→4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp	412:461	1→	442:443	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	5	57	theme	element-binding	944:958	arg1	protein					960:966	sterol regulatory element-binding protein 2	926:968	sterol regulatory element-binding protein 2 in hepatoma Huh7 cells	926:991	This polysaccharide significantly enhanced the intracellular protein expression of low-density lipoprotein receptor and proprotein convertase subtilisin/kexin type 9 (PCSK9) via regulating sterol regulatory element-binding protein 2 in hepatoma Huh7 cells.					
33662421	1	58	theme	novel	156:160	arg1	CM3-SII					195:201	CM3-SII	195:201	CM3-SII	195:201	One novel alkali-extracted polysaccharide, CM3-SII, was obtained from the fruiting body of C. militaris via column chromatography.					
33662421	1	58	theme	novel	156:160	arg1	polysaccharide					179:192	One novel alkali-extracted polysaccharide	152:192	One novel alkali-extracted polysaccharide	152:192	One novel alkali-extracted polysaccharide, CM3-SII, was obtained from the fruiting body of C. militaris via column chromatography.					
33662421	1	59	theme	militaris	246:254	arg1	body					235:238	the fruiting body	222:238	the fruiting body of C. militaris	222:254	One novel alkali-extracted polysaccharide, CM3-SII, was obtained from the fruiting body of C. militaris via column chromatography.					
33662421	0	60	theme	inhibitory	63:72	arg1	effect					74:79	PCSK9 secretion inhibitory effect	47:79	PCSK9 secretion inhibitory effect	47:79	Purification, structural characterization, and PCSK9 secretion inhibitory effect of the novel alkali-extracted polysaccharide from Cordyceps militaris.					
33662421	1	61	theme	alkali-extracted	162:177	arg1	CM3-SII					195:201	CM3-SII	195:201	CM3-SII	195:201	One novel alkali-extracted polysaccharide, CM3-SII, was obtained from the fruiting body of C. militaris via column chromatography.					
33662421	1	61	theme	alkali-extracted	162:177	arg1	polysaccharide					179:192	One novel alkali-extracted polysaccharide	152:192	One novel alkali-extracted polysaccharide	152:192	One novel alkali-extracted polysaccharide, CM3-SII, was obtained from the fruiting body of C. militaris via column chromatography.					
33662421	3	62	with	glycosyls	531:539	arg1	β-D-Galp					546:553	β-D-Galp	546:553	β-D-Galp	546:553	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	3	62	with	glycosyls	531:539	arg1	linked-β-D-Galf					562:576	(1→2) linked-β-D-Galf	556:576	(1→2) linked-β-D-Galf	556:576	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33662421	3	62	with	glycosyls	531:539	arg1	→2,6					583:586	→2,6	583:586	→2,6	583:586	The backbone of CM3-SII was composed of →4)-β-D-Manp(1→, →6)-β-D-Manp(1→, and →6)-α-D-Manp(1→ glycosyls, and branching at the O-4 positions of →6)-β-D-Manp(1→ glycosyls with β-D-Galp, (1→2) linked-β-D-Galf, and →2,6)-α-D-Manp(1→ residues.					
33650863	5	0	theme	glycans	1140:1146	arg1	increase					1108:1115	a significant increase	1094:1115	a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine	1094:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	5	1	theme	relative	1034:1041	arg1	quantity					1043:1050	the relative quantity	1030:1050	the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine	1030:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	1	2	gly	glycoprotein	75:86	arg1	glycoprotein					75:86	The glycoprotein uromodulin	71:97	The glycoprotein uromodulin (UMOD)	71:104	The glycoprotein uromodulin (UMOD) is the most abundant protein in urine, and N-glycans are critical for many biological functions of UMOD.					
33650863	7	3	theme	analytical	1387:1396	arg1	workflow					1398:1405	This analytical workflow	1382:1405	This analytical workflow	1382:1405	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	3	4	from	mL	515:516	arg1	donors					548:553	6 healthy adult donors	532:553	6 healthy adult donors (3 males and 3 females)	532:577	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	3	4	from	mL	515:516	arg1	analysis					599:606	a glycosylation analysis	583:606	a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	583:686	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	3	4	from	mL	515:516	arg1	isolation					498:506	UMOD isolation	493:506	UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females)	493:577	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	5	5	theme	neutral	1120:1126	arg1	glycans					1140:1146	neutral fucosylated glycans	1120:1146	neutral fucosylated glycans	1120:1146	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	3	6	theme	glycosylation	371:383	arg1	analysis					385:392	comprehensive glycosylation analysis	357:392	comprehensive glycosylation analysis of UMOD	357:400	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	6	7	theme	comprehensive	1321:1333	arg1	profiling					1344:1352	comprehensive N-glycan profiling	1321:1352	comprehensive N-glycan profiling of UMOD using nanoLC-MS/MS	1321:1379	To our knowledge, this is the first study to perform comprehensive N-glycan profiling of UMOD using nanoLC-MS/MS.					
33650863	5	8	with	glycans	1081:1087	arg1	increase					1108:1115	a significant increase	1094:1115	a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine	1094:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	3	9	theme	adult	542:546	arg1	donors					548:553	6 healthy adult donors	532:553	6 healthy adult donors (3 males and 3 females)	532:577	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	3	10	from	donors	548:553	arg1	urine					521:525	urine	521:525	urine from 6 healthy adult donors (3 males and 3 females)	521:577	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	3	10	from	donors	548:553	arg1	mL					515:516	5 mL	513:516	5 mL of urine from 6 healthy adult donors (3 males and 3 females)	513:577	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	5	11	theme	fucosylated	1128:1138	arg1	glycans					1140:1146	neutral fucosylated glycans	1120:1146	neutral fucosylated glycans	1120:1146	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	7	12	theme	patient	1526:1532	arg1	cohorts					1534:1540	larger patient cohorts	1519:1540	larger patient cohorts not only for UMOD interrogation but also for global glycan analysis	1519:1608	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	4	13	theme	purified	750:757	arg1	UMOD					759:762	the purified UMOD	746:762	the purified UMOD	746:762	In total, 212 N-glycan compositions were identified from the purified UMOD, and 17% were high-mannose glycans, 2% were afucosylated/asialylated, 3% were neutral fucosylated, 28% were sialylated (with no fucose), 46% were fucosylated and sialylated, and 4% were sulfated.					
33650863	0	14	theme	Uromodulin	0:9	arg1	Isolation					11:19	Uromodulin Isolation	0:19	Uromodulin Isolation	0:19	Uromodulin Isolation and Its N-Glycosylation Analysis by NanoLC-MS/MS.					
33650863	6	15	theme	N-glycan	1335:1342	arg1	profiling					1344:1352	comprehensive N-glycan profiling	1321:1352	comprehensive N-glycan profiling of UMOD using nanoLC-MS/MS	1321:1379	To our knowledge, this is the first study to perform comprehensive N-glycan profiling of UMOD using nanoLC-MS/MS.					
33650863	3	16	dep	donors	548:553	arg1	males					558:562	3 males	556:562	3 males	556:562	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	3	16	dep	donors	548:553	arg1	females					570:576	3 females	568:576	3 females	568:576	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	4	17	theme	212	699:701	arg1	compositions					712:723	212 N-glycan compositions	699:723	212 N-glycan compositions	699:723	In total, 212 N-glycan compositions were identified from the purified UMOD, and 17% were high-mannose glycans, 2% were afucosylated/asialylated, 3% were neutral fucosylated, 28% were sialylated (with no fucose), 46% were fucosylated and sialylated, and 4% were sulfated.					
33650863	4	18	gly	afucosylated/asialylated	808:831	arg1	%					801:801	2%	800:801	2%	800:801	In total, 212 N-glycan compositions were identified from the purified UMOD, and 17% were high-mannose glycans, 2% were afucosylated/asialylated, 3% were neutral fucosylated, 28% were sialylated (with no fucose), 46% were fucosylated and sialylated, and 4% were sulfated.					
33650863	1	19	theme	glycoprotein	75:86	arg1	UMOD					100:103	UMOD	100:103	UMOD	100:103	The glycoprotein uromodulin (UMOD) is the most abundant protein in urine, and N-glycans are critical for many biological functions of UMOD.					
33650863	1	19	theme	glycoprotein	75:86	arg1	uromodulin					88:97	The glycoprotein uromodulin	71:97	The glycoprotein uromodulin (UMOD)	71:104	The glycoprotein uromodulin (UMOD) is the most abundant protein in urine, and N-glycans are critical for many biological functions of UMOD.					
33650863	1	19	theme	glycoprotein	75:86	arg1	protein					127:133	the most abundant protein	109:133	the most abundant protein in urine	109:142	The glycoprotein uromodulin (UMOD) is the most abundant protein in urine, and N-glycans are critical for many biological functions of UMOD.					
33650863	5	20	theme	sialylated	1248:1257	arg1	glycans					1259:1265	the sialylated glycans	1244:1265	the sialylated glycans	1244:1265	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	3	21	theme	comprehensive	357:369	arg1	analysis					385:392	comprehensive glycosylation analysis	357:392	comprehensive glycosylation analysis of UMOD	357:400	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	5	22	from	increase	1108:1115	arg1	urine					1169:1173	the UMOD-depleted urine	1151:1173	the UMOD-depleted urine relative to the undepleted urine	1151:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	2	23	theme	valuable	259:266	arg1	information					268:278	valuable information	259:278	valuable information to understand the exact mechanisms of glycan-regulated functions	259:343	Comprehensive glycan profiling of UMOD provides valuable information to understand the exact mechanisms of glycan-regulated functions.					
33650863	5	24	theme	significant	1006:1016	arg1	decrease					1018:1025	a significant decrease	1004:1025	a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine	1004:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	5	25	from	decrease	1018:1025	arg1	quantity					1043:1050	the relative quantity	1030:1050	the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine	1030:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	1	26	from	protein	127:133	arg1	urine					138:142	urine	138:142	urine	138:142	The glycoprotein uromodulin (UMOD) is the most abundant protein in urine, and N-glycans are critical for many biological functions of UMOD.					
33650863	5	27	theme	glycans	1081:1087	arg1	quantity					1043:1050	the relative quantity	1030:1050	the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine	1030:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	3	28	theme	urine	407:411	arg1	samples					413:419	urine samples	407:419	urine samples with limited volumes	407:440	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	3	29	with	samples	413:419	arg1	volumes					434:440	limited volumes	426:440	limited volumes	426:440	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	4	30	theme	N-glycan	703:710	arg1	compositions					712:723	212 N-glycan compositions	699:723	212 N-glycan compositions	699:723	In total, 212 N-glycan compositions were identified from the purified UMOD, and 17% were high-mannose glycans, 2% were afucosylated/asialylated, 3% were neutral fucosylated, 28% were sialylated (with no fucose), 46% were fucosylated and sialylated, and 4% were sulfated.					
33650863	7	31	theme	global	1587:1592	arg1	analysis					1601:1608	global glycan analysis	1587:1608	global glycan analysis	1587:1608	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	4	32	gly	fucosylated	850:860	arg1	%					835:835	3%	834:835	3%	834:835	In total, 212 N-glycan compositions were identified from the purified UMOD, and 17% were high-mannose glycans, 2% were afucosylated/asialylated, 3% were neutral fucosylated, 28% were sialylated (with no fucose), 46% were fucosylated and sialylated, and 4% were sulfated.					
33650863	5	33	gly	fucosylated	1128:1138	arg1	glycans					1140:1146	neutral fucosylated glycans	1120:1146	neutral fucosylated glycans	1120:1146	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	2	34	theme	exact	298:302	arg1	mechanisms					304:313	the exact mechanisms	294:313	the exact mechanisms of glycan-regulated functions	294:343	Comprehensive glycan profiling of UMOD provides valuable information to understand the exact mechanisms of glycan-regulated functions.					
33650863	5	35	theme	little	1227:1232	arg1	impact					1234:1239	little impact	1227:1239	little impact	1227:1239	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	1	36	theme	many	176:179	arg1	functions					192:200	many biological functions	176:200	many biological functions of UMOD	176:208	The glycoprotein uromodulin (UMOD) is the most abundant protein in urine, and N-glycans are critical for many biological functions of UMOD.					
33650863	3	37	theme	UMOD	397:400	arg1	analysis					385:392	comprehensive glycosylation analysis	357:392	comprehensive glycosylation analysis of UMOD	357:400	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	5	38	theme	significant	1096:1106	arg1	increase					1108:1115	a significant increase	1094:1115	a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine	1094:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	3	39	gly	glycosylation	371:383	arg1	UMOD					397:400	UMOD	397:400	UMOD	397:400	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	3	40	theme	sensitive	623:631	arg1	approach					679:686	a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	614:686	a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	614:686	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	5	41	contain	had	1223:1225	arg2	impact					1234:1239	little impact	1227:1239	little impact	1227:1239	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	5	41	contain	had	1223:1225	arg1	depletion					1213:1221	depletion	1213:1221	depletion	1213:1221	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	5	42	theme	undepleted	1191:1200	arg1	urine					1202:1206	the undepleted urine	1187:1206	the undepleted urine	1187:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	1	43	theme	biological	181:190	arg1	functions					192:200	many biological functions	176:200	many biological functions of UMOD	176:208	The glycoprotein uromodulin (UMOD) is the most abundant protein in urine, and N-glycans are critical for many biological functions of UMOD.					
33650863	3	44	theme	limited	426:432	arg1	volumes					434:440	limited volumes	426:440	limited volumes	426:440	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	7	45	theme	glycan	1594:1599	arg1	analysis					1601:1608	global glycan analysis	1587:1608	global glycan analysis	1587:1608	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	7	46	theme	sample	1457:1462	arg1	size					1464:1467	limited sample size	1449:1467	limited sample size	1449:1467	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	5	47	theme	UMOD	987:990	arg1	isolation					974:982	isolation	974:982	isolation of UMOD	974:990	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	2	48	theme	glycan	225:230	arg1	profiling					232:240	Comprehensive glycan profiling	211:240	Comprehensive glycan profiling of UMOD	211:248	Comprehensive glycan profiling of UMOD provides valuable information to understand the exact mechanisms of glycan-regulated functions.					
33650863	7	49	theme	UMOD	1555:1558	arg1	interrogation					1560:1572	UMOD interrogation	1555:1572	UMOD interrogation	1555:1572	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	3	50	theme	streamlined	458:468	arg1	workflow					470:477	a streamlined workflow	456:477	a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	456:686	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	0	51	theme	N-Glycosylation	29:43	arg1	Analysis					45:52	Its N-Glycosylation Analysis	25:52	Its N-Glycosylation Analysis by NanoLC-MS/MS	25:68	Uromodulin Isolation and Its N-Glycosylation Analysis by NanoLC-MS/MS.					
33650863	5	52	theme	high-mannose	1055:1066	arg1	glycans					1081:1087	high-mannose and sulfated glycans	1055:1087	glycans	1081:1087	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	7	53	theme	pediatric	1478:1486	arg1	studies					1488:1494	pediatric studies	1478:1494	pediatric studies	1478:1494	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	2	54	theme	Comprehensive	211:223	arg1	profiling					232:240	Comprehensive glycan profiling	211:240	Comprehensive glycan profiling of UMOD	211:248	Comprehensive glycan profiling of UMOD provides valuable information to understand the exact mechanisms of glycan-regulated functions.					
33650863	4	55	dep	sialylated	872:881	arg1	fucose					892:897	no fucose	889:897	no fucose	889:897	In total, 212 N-glycan compositions were identified from the purified UMOD, and 17% were high-mannose glycans, 2% were afucosylated/asialylated, 3% were neutral fucosylated, 28% were sialylated (with no fucose), 46% were fucosylated and sialylated, and 4% were sulfated.					
33650863	7	56	theme	larger	1519:1524	arg1	cohorts					1534:1540	larger patient cohorts	1519:1540	larger patient cohorts not only for UMOD interrogation but also for global glycan analysis	1519:1608	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	7	57	theme	limited	1449:1455	arg1	size					1464:1467	limited sample size	1449:1467	limited sample size	1449:1467	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	7	58	with	studies	1436:1442	arg1	size					1464:1467	limited sample size	1449:1467	limited sample size	1449:1467	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	4	59	gly	sialylated	872:881	arg1	%					865:865	28%	863:865	28%	863:865	In total, 212 N-glycan compositions were identified from the purified UMOD, and 17% were high-mannose glycans, 2% were afucosylated/asialylated, 3% were neutral fucosylated, 28% were sialylated (with no fucose), 46% were fucosylated and sialylated, and 4% were sulfated.					
33650863	5	60	theme	sulfated	1072:1079	arg1	glycans					1081:1087	high-mannose and sulfated glycans	1055:1087	glycans	1081:1087	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	3	61	theme	UMOD	493:496	arg1	isolation					498:506	UMOD isolation	493:506	UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females)	493:577	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	3	62	theme	based	663:667	arg1	approach					679:686	a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	614:686	a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	614:686	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	7	63	dep	such	1470:1473	arg1	as					1475:1476	as	1475:1476	as	1475:1476	This analytical workflow would be very beneficial for studies with limited sample size, such as pediatric studies, and can be applied to larger patient cohorts not only for UMOD interrogation but also for global glycan analysis.					
33650863	4	64	gly	fucosylated	910:920	arg1	%					903:903	46%	901:903	46%	901:903	In total, 212 N-glycan compositions were identified from the purified UMOD, and 17% were high-mannose glycans, 2% were afucosylated/asialylated, 3% were neutral fucosylated, 28% were sialylated (with no fucose), 46% were fucosylated and sialylated, and 4% were sulfated.					
33650863	3	65	theme	glycomics	669:677	arg1	approach					679:686	a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	614:686	a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	614:686	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	4	66	gly	sialylated	926:935	arg1	%					903:903	46%	901:903	46%	901:903	In total, 212 N-glycan compositions were identified from the purified UMOD, and 17% were high-mannose glycans, 2% were afucosylated/asialylated, 3% were neutral fucosylated, 28% were sialylated (with no fucose), 46% were fucosylated and sialylated, and 4% were sulfated.					
33650863	1	67	theme	abundant	118:125	arg1	protein					127:133	the most abundant protein	109:133	the most abundant protein in urine	109:142	The glycoprotein uromodulin (UMOD) is the most abundant protein in urine, and N-glycans are critical for many biological functions of UMOD.					
33650863	1	67	theme	abundant	118:125	arg1	uromodulin					88:97	The glycoprotein uromodulin	71:97	The glycoprotein uromodulin (UMOD)	71:104	The glycoprotein uromodulin (UMOD) is the most abundant protein in urine, and N-glycans are critical for many biological functions of UMOD.					
33650863	1	68	theme	UMOD	205:208	arg1	functions					192:200	many biological functions	176:200	many biological functions of UMOD	176:208	The glycoprotein uromodulin (UMOD) is the most abundant protein in urine, and N-glycans are critical for many biological functions of UMOD.					
33650863	3	69	theme	healthy	534:540	arg1	donors					548:553	6 healthy adult donors	532:553	6 healthy adult donors (3 males and 3 females)	532:577	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	3	70	theme	reproducible	637:648	arg1	approach					679:686	a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	614:686	a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	614:686	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	2	71	theme	UMOD	245:248	arg1	profiling					232:240	Comprehensive glycan profiling	211:240	Comprehensive glycan profiling of UMOD	211:248	Comprehensive glycan profiling of UMOD provides valuable information to understand the exact mechanisms of glycan-regulated functions.					
33650863	2	72	theme	glycan-regulated	318:333	arg1	functions					335:343	glycan-regulated functions	318:343	glycan-regulated functions	318:343	Comprehensive glycan profiling of UMOD provides valuable information to understand the exact mechanisms of glycan-regulated functions.					
33650863	2	73	theme	functions	335:343	arg1	mechanisms					304:313	the exact mechanisms	294:313	the exact mechanisms of glycan-regulated functions	294:343	Comprehensive glycan profiling of UMOD provides valuable information to understand the exact mechanisms of glycan-regulated functions.					
33650863	6	74	theme	first	1298:1302	arg1	study					1304:1308	the first study	1294:1308	the first study to perform comprehensive N-glycan profiling of UMOD using nanoLC-MS/MS	1294:1379	To our knowledge, this is the first study to perform comprehensive N-glycan profiling of UMOD using nanoLC-MS/MS.					
33650863	6	74	theme	first	1298:1302	arg1	this					1286:1289	this	1286:1289	this	1286:1289	To our knowledge, this is the first study to perform comprehensive N-glycan profiling of UMOD using nanoLC-MS/MS.					
33650863	3	75	theme	nanoLC-MS/MS	650:661	arg1	approach					679:686	a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	614:686	a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	614:686	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	4	76	theme	high-mannose	778:789	arg1	glycans					791:797	high-mannose glycans	778:797	high-mannose glycans	778:797	In total, 212 N-glycan compositions were identified from the purified UMOD, and 17% were high-mannose glycans, 2% were afucosylated/asialylated, 3% were neutral fucosylated, 28% were sialylated (with no fucose), 46% were fucosylated and sialylated, and 4% were sulfated.					
33650863	3	77	theme	urine	521:525	arg1	mL					515:516	5 mL	513:516	5 mL of urine from 6 healthy adult donors (3 males and 3 females)	513:577	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	5	78	theme	UMOD-depleted	1155:1167	arg1	urine					1169:1173	the UMOD-depleted urine	1151:1173	the UMOD-depleted urine relative to the undepleted urine	1151:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	5	79	gly	sialylated	1248:1257	arg1	glycans					1259:1265	the sialylated glycans	1244:1265	the sialylated glycans	1244:1265	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
33650863	3	80	theme	glycosylation	585:597	arg1	analysis					599:606	a glycosylation analysis	583:606	a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach	583:686	To perform comprehensive glycosylation analysis of UMOD from urine samples with limited volumes, we developed a streamlined workflow that included UMOD isolation from 5 mL of urine from 6 healthy adult donors (3 males and 3 females) and a glycosylation analysis using a highly sensitive and reproducible nanoLC-MS/MS based glycomics approach.					
33650863	6	81	theme	UMOD	1357:1360	arg1	profiling					1344:1352	comprehensive N-glycan profiling	1321:1352	comprehensive N-glycan profiling of UMOD using nanoLC-MS/MS	1321:1379	To our knowledge, this is the first study to perform comprehensive N-glycan profiling of UMOD using nanoLC-MS/MS.					
33650863	5	82	theme	relative	1175:1182	arg1	urine					1169:1173	the UMOD-depleted urine	1151:1173	the UMOD-depleted urine relative to the undepleted urine	1151:1206	We found that isolation of UMOD resulted in a significant decrease in the relative quantity of high-mannose and sulfated glycans with a significant increase of neutral fucosylated glycans in the UMOD-depleted urine relative to the undepleted urine, but depletion had little impact on the sialylated glycans.					
34350945	9	0	theme	candidate	1800:1808	arg1	glycosyltransferases					1775:1794	glycosyltransferases	1775:1794	glycosyltransferases	1775:1794	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	9	0	theme	candidate	1800:1808	arg1	biomarkers					1810:1819	candidate biomarkers	1800:1819	candidate biomarkers for grading bladder cancers	1800:1847	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	9	0	theme	candidate	1800:1808	arg1	glycans					1763:1769	glycans	1763:1769	glycans	1763:1769	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	2	1	theme	low-grade	332:340	arg1	cancers					365:371	low-grade and high-grade bladder cancers	332:371	low-grade and high-grade bladder cancers	332:371	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	8	2	theme	cognate	1554:1560	arg1	glycosyltransferases					1562:1581	their cognate glycosyltransferases	1548:1581	their cognate glycosyltransferases	1548:1581	These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes.					
34350945	8	2	theme	cognate	1554:1560	arg1	genes					1620:1624	α1-3/4-fucosyltransferase genes	1594:1624	α1-3/4-fucosyltransferase genes	1594:1624	These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes.					
34350945	3	3	dep	lines	624:628	arg1	A/T/N					723:727	A/T/N	723:727	A/T/N	723:727	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	3	dep	lines	624:628	arg1	grade-3					638:644	grade-3	638:644	grade-3	638:644	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	3	dep	lines	624:628	arg1	TCCSUP					666:671	TCCSUP	666:671	TCCSUP	666:671	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	3	dep	lines	624:628	arg1	T24					648:650	T24 (grade-3)	648:660	T24 (grade-3)	648:660	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	3	dep	lines	624:628	arg1	grade-4					674:680	grade-4	674:680	grade-4	674:680	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	3	dep	lines	624:628	arg1	lines					624:628	three high-grade bladder cancer cell lines	587:628	three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N	587:727	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	3	dep	lines	624:628	arg1	J82COT					630:635	J82COT	630:635	J82COT	630:635	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	9	4	theme	bladder	1833:1839	arg1	cancers					1841:1847	grading bladder cancers	1825:1847	grading bladder cancers	1825:1847	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	4	5	dep	lines	939:943	arg1	SW780					959:963	SW780	959:963	SW780	959:963	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	5	dep	lines	939:943	arg1	5637					950:953	5637	950:953	5637	950:953	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	5	dep	lines	939:943	arg1	RT4					945:947	RT4	945:947	RT4	945:947	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	9	6	theme	low-	1709:1712	arg1	cells					1744:1748	low- and high-grade bladder cancer cells	1709:1748	low- and high-grade bladder cancer cells	1709:1748	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	8	7	theme	α1-3/4-fucosyltransferase	1594:1618	arg1	genes					1620:1624	α1-3/4-fucosyltransferase genes	1594:1624	α1-3/4-fucosyltransferase genes	1594:1624	These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes.					
34350945	8	8	from	differences	1459:1469	arg1	expression					1481:1490	glycan expression	1474:1490	glycan expression	1474:1490	These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes.					
34350945	3	9	theme	cell	518:521	arg1	lines					523:527	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	4	10	theme	Lewis-X	1004:1010	arg1	Lex					1021:1023	Lex	1021:1023	Lex	1021:1023	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	10	theme	Lewis-X	1004:1010	arg1	antigen					1012:1018	the fucosylated Lewis-X antigen	988:1018	the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R)	988:1059	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	10	theme	Lewis-X	1004:1010	arg1	GlcNAcβ1-R					1049:1058	Galβ1-4(Fucα1-3)GlcNAcβ1-R	1033:1058	Galβ1-4(Fucα1-3)GlcNAcβ1-R	1033:1058	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	3	11	theme	primary	689:695	arg1	cells					716:720	primary bladder epithelial cells	689:720	primary bladder epithelial cells	689:720	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	9	12	theme	expression	1681:1690	arg1	profiles					1692:1699	gene expression profiles	1676:1699	gene expression profiles	1676:1699	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	4	13	theme	approaches	791:800	arg1	immunofluorescence					828:845	immunofluorescence	828:845	immunofluorescence	828:845	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	13	theme	approaches	791:800	arg1	analysis					878:885	gene expression analysis	862:885	gene expression analysis	862:885	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	13	theme	approaches	791:800	arg1	approaches					791:800	approaches	791:800	approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis	791:885	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	13	theme	approaches	791:800	arg1	glycomics					848:856	glycomics	848:856	glycomics	848:856	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	13	theme	approaches	791:800	arg1	cytometry					817:825	flow cytometry	812:825	flow cytometry	812:825	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	13	theme	approaches	791:800	arg1	variety					780:786	a variety	778:786	a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis	778:885	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	14	theme	Fucα1-3	1041:1047	arg1	antigen					1012:1018	the fucosylated Lewis-X antigen	988:1018	the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R)	988:1059	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	14	theme	Fucα1-3	1041:1047	arg1	GlcNAcβ1-R					1049:1058	Galβ1-4(Fucα1-3)GlcNAcβ1-R	1033:1058	Galβ1-4(Fucα1-3)GlcNAcβ1-R	1033:1058	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	6	15	from	differences	1251:1261	arg1	sialylation					1283:1293	sialylation	1283:1293	sialylation	1283:1293	Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types.					
34350945	6	15	from	differences	1251:1261	arg1	fucosylation					1266:1277	fucosylation	1266:1277	fucosylation	1266:1277	Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types.					
34350945	3	16	theme	cell	758:761	arg1	control					763:769	a normal bladder cell control	741:769	a normal bladder cell control	741:769	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	4	17	theme	flow	812:815	arg1	cytometry					817:825	flow cytometry	812:825	flow cytometry	812:825	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	5	18	theme	TCCSUP	1135:1140	arg1	cells					1142:1146	T24 and TCCSUP cells	1127:1146	T24 and TCCSUP cells	1127:1146	T24 and TCCSUP cells also lack Lex, whereas J82COT cells express low levels of Lex.					
34350945	3	19	theme	normal	743:748	arg1	control					763:769	a normal bladder cell control	741:769	a normal bladder cell control	741:769	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	20	theme	protein	462:468	arg1	glycosylation					470:482	protein glycosylation	462:482	protein glycosylation	462:482	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	21	theme	epithelial	705:714	arg1	cells					716:720	primary bladder epithelial cells	689:720	primary bladder epithelial cells	689:720	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	0	22	theme	low-grade	72:80	arg1	lines					120:124	low-grade versus high-grade bladder cancer cell lines	72:124	low-grade versus high-grade bladder cancer cell lines	72:124	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.					
34350945	4	23	dep	Lex	1021:1023	arg1	CD15					1026:1029	CD15	1026:1029	CD15	1026:1029	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	9	24	theme	glycan	1654:1659	arg1	structures					1661:1670	glycan structures	1654:1670	glycan structures	1654:1670	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	4	25	theme	Galβ1-4	1033:1039	arg1	antigen					1012:1018	the fucosylated Lewis-X antigen	988:1018	the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R)	988:1059	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	25	theme	Galβ1-4	1033:1039	arg1	GlcNAcβ1-R					1049:1058	Galβ1-4(Fucα1-3)GlcNAcβ1-R	1033:1058	Galβ1-4(Fucα1-3)GlcNAcβ1-R	1033:1058	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	0	26	theme	high-grade	89:98	arg1	lines					120:124	low-grade versus high-grade bladder cancer cell lines	72:124	low-grade versus high-grade bladder cancer cell lines	72:124	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.					
34350945	1	27	theme	diagnosed	171:179	arg1	worldwide					188:196	the ninth most frequently diagnosed cancer worldwide	145:196	the ninth most frequently diagnosed cancer worldwide	145:196	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	1	27	theme	diagnosed	171:179	arg1	cancer					135:140	Bladder cancer	127:140	Bladder cancer	127:140	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	4	28	theme	epithelial	1083:1092	arg1	cells					1100:1104	normal bladder epithelial A/T/N cells	1068:1104	normal bladder epithelial A/T/N cells	1068:1104	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	5	29	theme	T24	1127:1129	arg1	cells					1142:1146	T24 and TCCSUP cells	1127:1146	T24 and TCCSUP cells	1127:1146	T24 and TCCSUP cells also lack Lex, whereas J82COT cells express low levels of Lex.					
34350945	0	30	theme	cancer	108:113	arg1	lines					120:124	low-grade versus high-grade bladder cancer cell lines	72:124	low-grade versus high-grade bladder cancer cell lines	72:124	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.					
34350945	6	31	gly	fucosylation	1266:1277	arg1	N-glycans					1298:1306	N-glycans	1298:1306	N-glycans	1298:1306	Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types.					
34350945	4	32	theme	expression	867:876	arg1	analysis					878:885	gene expression analysis	862:885	gene expression analysis	862:885	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	33	theme	cell	934:937	arg1	lines					939:943	the low-grade bladder cancer cell lines	905:943	the low-grade bladder cancer cell lines RT4, 5637 and SW780	905:963	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	5	34	theme	low	1192:1194	arg1	levels					1196:1201	low levels	1192:1201	low levels of Lex	1192:1208	T24 and TCCSUP cells also lack Lex, whereas J82COT cells express low levels of Lex.					
34350945	0	35	theme	Major	0:4	arg1	differences					6:16	Major differences	0:16	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.	0:125	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.					
34350945	2	36	theme	surface	427:433	arg1	glycoproteins					435:447	surface glycoproteins	427:447	surface glycoproteins	427:447	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	4	37	theme	normal	1068:1073	arg1	cells					1100:1104	normal bladder epithelial A/T/N cells	1068:1104	normal bladder epithelial A/T/N cells	1068:1104	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	2	38	theme	glycans	416:422	arg1	expression					402:411	expression	402:411	expression of glycans in surface glycoproteins	402:447	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	1	39	theme	disease	279:285	arg1	staging					249:255	staging	249:255	staging	249:255	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	1	39	theme	disease	279:285	arg1	prognosis					261:269	prognosis	261:269	prognosis	261:269	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	7	40	theme	core	1395:1398	arg1	O-glycans					1408:1416	core 2-based O-glycans	1395:1416	core 2-based O-glycans that are lacking in the T24 cells	1395:1450	O-glycans are highly differentiated, as RT4 cells synthesize core 2-based O-glycans that are lacking in the T24 cells.					
34350945	6	41	gly	sialylation	1283:1293	arg1	N-glycans					1298:1306	N-glycans	1298:1306	N-glycans	1298:1306	Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types.					
34350945	9	42	theme	bladder	1729:1735	arg1	cells					1744:1748	low- and high-grade bladder cancer cells	1709:1748	low- and high-grade bladder cancer cells	1709:1748	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	3	43	theme	cancer	612:617	arg1	lines					624:628	three high-grade bladder cancer cell lines	587:628	three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N	587:727	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	43	theme	cancer	612:617	arg1	TCCSUP					666:671	TCCSUP	666:671	TCCSUP	666:671	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	43	theme	cancer	612:617	arg1	T24					648:650	T24 (grade-3)	648:660	T24 (grade-3)	648:660	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	43	theme	cancer	612:617	arg1	J82COT					630:635	J82COT	630:635	J82COT	630:635	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	44	gly	glycosylation	470:482	arg1	SW780					566:570	SW780	566:570	SW780	566:570	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	44	gly	glycosylation	470:482	arg1	lines					523:527	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	44	gly	glycosylation	470:482	arg1	5637					546:549	5637	546:549	5637	546:549	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	44	gly	glycosylation	470:482	arg1	grade-2					552:558	grade-2	552:558	grade-2	552:558	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	44	gly	glycosylation	470:482	arg1	grade-1					573:579	grade-1	573:579	grade-1	573:579	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	44	gly	glycosylation	470:482	arg1	TCCSUP					666:671	TCCSUP	666:671	TCCSUP	666:671	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	44	gly	glycosylation	470:482	arg1	T24					648:650	T24 (grade-3)	648:660	T24 (grade-3)	648:660	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	44	gly	glycosylation	470:482	arg1	lines					624:628	three high-grade bladder cancer cell lines	587:628	three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N	587:727	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	44	gly	glycosylation	470:482	arg1	J82COT					630:635	J82COT	630:635	J82COT	630:635	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	4	45	theme	bladder	919:925	arg1	lines					939:943	the low-grade bladder cancer cell lines	905:943	the low-grade bladder cancer cell lines RT4, 5637 and SW780	905:963	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	3	46	theme	high-grade	593:602	arg1	lines					624:628	three high-grade bladder cancer cell lines	587:628	three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N	587:727	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	46	theme	high-grade	593:602	arg1	TCCSUP					666:671	TCCSUP	666:671	TCCSUP	666:671	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	46	theme	high-grade	593:602	arg1	T24					648:650	T24 (grade-3)	648:660	T24 (grade-3)	648:660	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	46	theme	high-grade	593:602	arg1	J82COT					630:635	J82COT	630:635	J82COT	630:635	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	47	theme	low-grade	493:501	arg1	lines					523:527	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	6	48	theme	major	1245:1249	arg1	differences					1251:1261	other major differences	1239:1261	other major differences in fucosylation and sialylation of N-glycans between these cell types	1239:1331	Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types.					
34350945	2	49	from	differences	387:397	arg1	expression					402:411	expression	402:411	expression of glycans in surface glycoproteins	402:447	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	4	50	theme	high	973:976	arg1	levels					978:983	high levels	973:983	high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R)	973:1059	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	51	theme	Lex	1111:1113	arg1	expression					1115:1124	Lex expression	1111:1124	Lex expression	1111:1124	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	9	52	from	differences	1639:1649	arg1	structures					1661:1670	glycan structures	1654:1670	glycan structures	1654:1670	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	9	52	from	differences	1639:1649	arg1	profiles					1692:1699	gene expression profiles	1676:1699	gene expression profiles	1676:1699	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	2	53	from	expression	402:411	arg1	glycoproteins					435:447	surface glycoproteins	427:447	surface glycoproteins	427:447	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	2	54	theme	bladder	357:363	arg1	cancers					365:371	low-grade and high-grade bladder cancers	332:371	low-grade and high-grade bladder cancers	332:371	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	8	55	theme	RNA	1523:1525	arg1	levels					1538:1543	RNA expression levels	1523:1543	RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes	1523:1624	These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes.					
34350945	1	56	theme	new	230:232	arg1	biomarkers					234:243	new biomarkers	230:243	new biomarkers for staging and prognosis of this disease	230:285	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	5	57	dep	lack	1153:1156	arg1	whereas					1163:1169	whereas	1163:1169	whereas	1163:1169	T24 and TCCSUP cells also lack Lex, whereas J82COT cells express low levels of Lex.					
34350945	3	58	theme	cancer	511:516	arg1	lines					523:527	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	6	59	theme	cell	1322:1325	arg1	types					1327:1331	these cell types	1316:1331	these cell types	1316:1331	Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types.					
34350945	8	60	theme	glycan	1474:1479	arg1	expression					1481:1490	glycan expression	1474:1490	glycan expression	1474:1490	These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes.					
34350945	3	61	with	T24	648:650	arg1	cells					716:720	primary bladder epithelial cells	689:720	primary bladder epithelial cells	689:720	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	2	62	attach	derived	319:325	arg1	cancers					365:371	low-grade and high-grade bladder cancers	332:371	low-grade and high-grade bladder cancers	332:371	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	2	62	attach	derived	319:325	arg2	lines					313:317	cell lines	308:317	cell lines derived from low-grade and high-grade bladder cancers	308:371	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	9	63	theme	grading	1825:1831	arg1	cancers					1841:1847	grading bladder cancers	1825:1847	grading bladder cancers	1825:1847	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	8	64	theme	glycosyltransferases	1562:1581	arg1	levels					1538:1543	RNA expression levels	1523:1543	RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes	1523:1624	These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes.					
34350945	1	65	theme	cancer	181:186	arg1	worldwide					188:196	the ninth most frequently diagnosed cancer worldwide	145:196	the ninth most frequently diagnosed cancer worldwide	145:196	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	1	65	theme	cancer	181:186	arg1	cancer					135:140	Bladder cancer	127:140	Bladder cancer	127:140	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	6	66	theme	Glycomics	1211:1219	arg1	analyses					1221:1228	Glycomics analyses	1211:1228	Glycomics analyses	1211:1228	Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types.					
34350945	4	67	theme	fucosylated	992:1002	arg1	Lex					1021:1023	Lex	1021:1023	Lex	1021:1023	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	67	theme	fucosylated	992:1002	arg1	antigen					1012:1018	the fucosylated Lewis-X antigen	988:1018	the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R)	988:1059	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	67	theme	fucosylated	992:1002	arg1	GlcNAcβ1-R					1049:1058	Galβ1-4(Fucα1-3)GlcNAcβ1-R	1033:1058	Galβ1-4(Fucα1-3)GlcNAcβ1-R	1033:1058	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	68	theme	antigen	1012:1018	arg1	levels					978:983	high levels	973:983	high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R)	973:1059	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	3	69	theme	bladder	697:703	arg1	cells					716:720	primary bladder epithelial cells	689:720	primary bladder epithelial cells	689:720	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	1	70	theme	Bladder	127:133	arg1	cancer					135:140	Bladder cancer	127:140	Bladder cancer	127:140	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	1	70	theme	Bladder	127:133	arg1	worldwide					188:196	the ninth most frequently diagnosed cancer worldwide	145:196	the ninth most frequently diagnosed cancer worldwide	145:196	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	0	71	dep	glycosylation	21:33	arg1	expression					58:67	expression	58:67	expression	58:67	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.					
34350945	7	72	theme	T24	1442:1444	arg1	cells					1446:1450	the T24 cells	1438:1450	the T24 cells	1438:1450	O-glycans are highly differentiated, as RT4 cells synthesize core 2-based O-glycans that are lacking in the T24 cells.					
34350945	9	73	theme	major	1633:1637	arg1	differences					1639:1649	These major differences	1627:1649	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells	1627:1748	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	6	74	theme	other	1239:1243	arg1	differences					1251:1261	other major differences	1239:1261	other major differences in fucosylation and sialylation of N-glycans between these cell types	1239:1331	Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types.					
34350945	1	75	theme	ninth	149:153	arg1	worldwide					188:196	the ninth most frequently diagnosed cancer worldwide	145:196	the ninth most frequently diagnosed cancer worldwide	145:196	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	1	75	theme	ninth	149:153	arg1	cancer					135:140	Bladder cancer	127:140	Bladder cancer	127:140	Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease.					
34350945	3	76	theme	bladder	750:756	arg1	control					763:769	a normal bladder cell control	741:769	a normal bladder cell control	741:769	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	0	77	theme	bladder	100:106	arg1	lines					120:124	low-grade versus high-grade bladder cancer cell lines	72:124	low-grade versus high-grade bladder cancer cell lines	72:124	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.					
34350945	0	78	from	differences	6:16	arg1	fucosyltransferase					39:56	fucosyltransferase	39:56	fucosyltransferase	39:56	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.					
34350945	0	78	from	differences	6:16	arg1	glycosylation					21:33	glycosylation	21:33	glycosylation	21:33	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.					
34350945	0	78	from	differences	6:16	arg1	lines					120:124	low-grade versus high-grade bladder cancer cell lines	72:124	low-grade versus high-grade bladder cancer cell lines	72:124	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.					
34350945	4	79	theme	A/T/N	1094:1098	arg1	cells					1100:1104	normal bladder epithelial A/T/N cells	1068:1104	normal bladder epithelial A/T/N cells	1068:1104	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	0	80	theme	cell	115:118	arg1	lines					120:124	low-grade versus high-grade bladder cancer cell lines	72:124	low-grade versus high-grade bladder cancer cell lines	72:124	Major differences in glycosylation and fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.					
34350945	9	81	theme	gene	1676:1679	arg1	profiles					1692:1699	gene expression profiles	1676:1699	gene expression profiles	1676:1699	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	5	82	theme	J82COT	1171:1176	arg1	cells					1178:1182	J82COT cells	1171:1182	J82COT cells	1171:1182	T24 and TCCSUP cells also lack Lex, whereas J82COT cells express low levels of Lex.					
34350945	4	83	gly	fucosylated	992:1002	arg1	Lex					1021:1023	Lex	1021:1023	Lex	1021:1023	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	83	gly	fucosylated	992:1002	arg1	antigen					1012:1018	the fucosylated Lewis-X antigen	988:1018	the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R)	988:1059	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	83	gly	fucosylated	992:1002	arg1	GlcNAcβ1-R					1049:1058	Galβ1-4(Fucα1-3)GlcNAcβ1-R	1033:1058	Galβ1-4(Fucα1-3)GlcNAcβ1-R	1033:1058	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	84	theme	gene	862:865	arg1	analysis					878:885	gene expression analysis	862:885	gene expression analysis	862:885	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	8	85	from	differences	1508:1518	arg1	levels					1538:1543	RNA expression levels	1523:1543	RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes	1523:1624	These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes.					
34350945	2	86	theme	major	381:385	arg1	differences					387:397	major differences	381:397	major differences in expression of glycans in surface glycoproteins	381:447	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	6	87	theme	N-glycans	1298:1306	arg1	sialylation					1283:1293	sialylation	1283:1293	sialylation	1283:1293	Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types.					
34350945	6	87	theme	N-glycans	1298:1306	arg1	fucosylation					1266:1277	fucosylation	1266:1277	fucosylation	1266:1277	Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types.					
34350945	4	88	theme	cancer	927:932	arg1	lines					939:943	the low-grade bladder cancer cell lines	905:943	the low-grade bladder cancer cell lines RT4, 5637 and SW780	905:963	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	4	89	theme	bladder	1075:1081	arg1	cells					1100:1104	normal bladder epithelial A/T/N cells	1068:1104	normal bladder epithelial A/T/N cells	1068:1104	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	3	90	dep	lines	523:527	arg1	grade-1-2					534:542	grade-1-2	534:542	grade-1-2	534:542	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	90	dep	lines	523:527	arg1	RT4					529:531	RT4	529:531	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	9	91	theme	high-grade	1718:1727	arg1	cells					1744:1748	low- and high-grade bladder cancer cells	1709:1748	low- and high-grade bladder cancer cells	1709:1748	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	3	92	theme	bladder	604:610	arg1	lines					624:628	three high-grade bladder cancer cell lines	587:628	three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N	587:727	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	92	theme	bladder	604:610	arg1	TCCSUP					666:671	TCCSUP	666:671	TCCSUP	666:671	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	92	theme	bladder	604:610	arg1	T24					648:650	T24 (grade-3)	648:660	T24 (grade-3)	648:660	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	92	theme	bladder	604:610	arg1	J82COT					630:635	J82COT	630:635	J82COT	630:635	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	7	93	theme	2-based	1400:1406	arg1	O-glycans					1408:1416	core 2-based O-glycans	1395:1416	core 2-based O-glycans that are lacking in the T24 cells	1395:1450	O-glycans are highly differentiated, as RT4 cells synthesize core 2-based O-glycans that are lacking in the T24 cells.					
34350945	9	94	theme	cancer	1737:1742	arg1	cells					1744:1748	low- and high-grade bladder cancer cells	1709:1748	low- and high-grade bladder cancer cells	1709:1748	These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.					
34350945	4	95	theme	low-grade	909:917	arg1	lines					939:943	the low-grade bladder cancer cell lines	905:943	the low-grade bladder cancer cell lines RT4, 5637 and SW780	905:963	Using a variety of approaches including flow cytometry, immunofluorescence, glycomics and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637 and SW780 express high levels of the fucosylated Lewis-X antigen (Lex, CD15) (Galβ1-4(Fucα1-3)GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression.					
34350945	3	96	theme	bladder	503:509	arg1	lines					523:527	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	three low-grade bladder cancer cell lines RT4 (grade-1-2)	487:543	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	7	97	theme	RT4	1374:1376	arg1	cells					1378:1382	RT4 cells	1374:1382	RT4 cells	1374:1382	O-glycans are highly differentiated, as RT4 cells synthesize core 2-based O-glycans that are lacking in the T24 cells.					
34350945	2	98	gly	glycoproteins	435:447	arg1	glycoproteins					435:447	surface glycoproteins	427:447	surface glycoproteins	427:447	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	3	99	theme	cell	619:622	arg1	lines					624:628	three high-grade bladder cancer cell lines	587:628	three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N	587:727	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	99	theme	cell	619:622	arg1	TCCSUP					666:671	TCCSUP	666:671	TCCSUP	666:671	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	99	theme	cell	619:622	arg1	T24					648:650	T24 (grade-3)	648:660	T24 (grade-3)	648:660	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	3	99	theme	cell	619:622	arg1	J82COT					630:635	J82COT	630:635	J82COT	630:635	We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3) and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control.					
34350945	2	100	theme	cell	308:311	arg1	lines					313:317	cell lines	308:317	cell lines derived from low-grade and high-grade bladder cancers	308:371	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
34350945	8	101	theme	expression	1527:1536	arg1	levels					1538:1543	RNA expression levels	1523:1543	RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes	1523:1624	These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes.					
34350945	5	102	theme	Lex	1206:1208	arg1	levels					1196:1201	low levels	1192:1201	low levels of Lex	1192:1208	T24 and TCCSUP cells also lack Lex, whereas J82COT cells express low levels of Lex.					
34350945	2	103	theme	high-grade	346:355	arg1	cancers					365:371	low-grade and high-grade bladder cancers	332:371	low-grade and high-grade bladder cancers	332:371	Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins.					
32502344	4	0	theme	protein	630:636	arg1	level					638:642	the cancer-related membrane protein level	602:642	the cancer-related membrane protein level	602:642	Slightly aberrant expression of certain O-glycans can significantly affect cancer progression, especially at the cancer-related membrane protein level.					
32502344	10	1	theme	cleavage	1464:1471	arg1	site					1473:1476	a tryptic cleavage site	1454:1476	a tryptic cleavage site	1454:1476	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	14	2	from	Gal/GalNAc	1962:1971	arg1	lines					2007:2011	three human breast cancer cell lines	1976:2011	three human breast cancer cell lines	1976:2011	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	6	3	theme	protein-specific	878:893	arg1	glycosylation					895:907	protein-specific glycosylation	878:907	protein-specific glycosylation	878:907	However, few assays have been reported for the in situ detection of protein-specific glycosylation to date.					
32502344	14	4	from	pairs	2020:2024	arg1	lines					2007:2011	three human breast cancer cell lines	1976:2011	three human breast cancer cell lines	1976:2011	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	5	5	theme	protein-specific	682:697	arg1	glycosylation					699:711	protein-specific glycosylation	682:711	protein-specific glycosylation	682:711	To collect biological information on protein-specific glycosylation and further explore clinical applications, quantitative detection of glycosylation is essential.					
32502344	9	6	theme	DNA	1290:1292	arg1	sequence					1294:1301	a capture DNA sequence	1280:1301	a capture DNA sequence	1280:1301	The protein probe contained an aptamer for MUC1 protein recognition and a capture DNA sequence.					
32502344	1	7	theme	cellular	200:207	arg1	processes					209:217	cellular processes	200:217	cellular processes	200:217	Protein glycosylation is a prevalent post-translational modification that mediates a variety of cellular processes.					
32502344	13	8	theme	Gal/GalNAc	1865:1874	arg1	Gal/GalNAc					1865:1874	MUC1-specific terminal Gal/GalNAc	1842:1874	MUC1-specific terminal Gal/GalNAc	1842:1874	The mass response of the reporter peptide represented the amount of MUC1-specific terminal Gal/GalNAc.					
32502344	13	8	theme	Gal/GalNAc	1865:1874	arg1	amount					1832:1837	the amount	1828:1837	the amount of MUC1-specific terminal Gal/GalNAc	1828:1874	The mass response of the reporter peptide represented the amount of MUC1-specific terminal Gal/GalNAc.					
32502344	6	9	theme	in	857:858	arg1	detection					865:873	the in situ detection	853:873	the in situ detection of protein-specific glycosylation to date	853:915	However, few assays have been reported for the in situ detection of protein-specific glycosylation to date.					
32502344	8	10	theme	dual-probe	1121:1130	arg1	system					1171:1176	The dual-probe (i.e., protein probe and glycan probe) system	1117:1176	The dual-probe (i.e., protein probe and glycan probe) system	1117:1176	The dual-probe (i.e., protein probe and glycan probe) system was first designed and built.					
32502344	11	11	theme	restricted	1624:1633	arg1	space					1635:1639	a restricted space	1622:1639	a restricted space	1622:1639	Exonuclease III enabled recycling of the hybridization-dehybridization process in a restricted space.					
32502344	11	12	theme	process	1611:1617	arg1	recycling					1564:1572	recycling	1564:1572	recycling of the hybridization-dehybridization process	1564:1617	Exonuclease III enabled recycling of the hybridization-dehybridization process in a restricted space.					
32502344	14	13	theme	human	1982:1986	arg1	lines					2007:2011	three human breast cancer cell lines	1976:2011	three human breast cancer cell lines	1976:2011	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	14	14	dep	in	1918:1919	arg1	situ					1921:1924	situ	1921:1924	situ	1921:1924	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	14	15	from	detection	1926:1934	arg1	lines					2007:2011	three human breast cancer cell lines	1976:2011	three human breast cancer cell lines	1976:2011	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	14	16	theme	cancer	1995:2000	arg1	lines					2007:2011	three human breast cancer cell lines	1976:2011	three human breast cancer cell lines	1976:2011	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	12	17	theme	reporter	1655:1662	arg1	peptide					1664:1670	the reporter peptide	1651:1670	the reporter peptide	1651:1670	Finally, the reporter peptide was tryptically released and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32502344	13	18	theme	MUC1-specific	1842:1854	arg1	Gal/GalNAc					1865:1874	MUC1-specific terminal Gal/GalNAc	1842:1874	MUC1-specific terminal Gal/GalNAc	1842:1874	The mass response of the reporter peptide represented the amount of MUC1-specific terminal Gal/GalNAc.					
32502344	5	19	theme	quantitative	756:767	arg1	detection					769:777	quantitative detection	756:777	quantitative detection of glycosylation	756:794	To collect biological information on protein-specific glycosylation and further explore clinical applications, quantitative detection of glycosylation is essential.					
32502344	10	20	contain	had	1338:1340	arg2	sequence					1348:1355	a DNA sequence	1342:1355	a DNA sequence complementary to that of the capture DNA	1342:1396	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	10	20	contain	had	1338:1340	arg1	probe					1332:1336	the glycan probe	1321:1336	the glycan probe	1321:1336	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	10	20	contain	had	1338:1340	arg2	site					1473:1476	a tryptic cleavage site	1454:1476	a tryptic cleavage site	1454:1476	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	10	20	contain	had	1338:1340	arg2	peptide					1411:1417	a substrate peptide	1399:1417	a substrate peptide containing a reporter peptide	1399:1447	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	1	21	theme	prevalent	131:139	arg1	modification					160:171	a prevalent post-translational modification	129:171	a prevalent post-translational modification that mediates a variety of cellular processes	129:217	Protein glycosylation is a prevalent post-translational modification that mediates a variety of cellular processes.					
32502344	1	21	theme	prevalent	131:139	arg1	glycosylation					112:124	Protein glycosylation	104:124	Protein glycosylation	104:124	Protein glycosylation is a prevalent post-translational modification that mediates a variety of cellular processes.					
32502344	3	22	theme	various	319:325	arg1	types					341:345	the various glycosylation types	315:345	the various glycosylation types found in membrane proteins	315:372	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	10	23	theme	tryptic	1456:1462	arg1	site					1473:1476	a tryptic cleavage site	1454:1476	a tryptic cleavage site	1454:1476	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	10	24	theme	glycan	1325:1330	arg1	probe					1332:1336	the glycan probe	1321:1336	the glycan probe	1321:1336	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	12	25	theme	liquid	1715:1720	arg1	LC-MS/MS					1763:1770	LC-MS/MS	1763:1770	LC-MS/MS	1763:1770	Finally, the reporter peptide was tryptically released and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32502344	12	25	theme	liquid	1715:1720	arg1	spectrometry					1749:1760	liquid chromatography-tandem mass spectrometry	1715:1760	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1715:1771	Finally, the reporter peptide was tryptically released and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32502344	0	26	theme	Terminal	75:82	arg1	Gal/GalNAc					84:93	MUC1-Specific Terminal Gal/GalNAc	61:93	MUC1-Specific Terminal Gal/GalNAc	61:93	Dual-Probe Approach for Mass Spectrometric Quantification of MUC1-Specific Terminal Gal/GalNAc In Situ.					
32502344	7	27	theme	MUC1	1100:1103	arg1	Gal/GalNAc					1085:1094	Gal/GalNAc	1085:1094	Gal/GalNAc	1085:1094	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
32502344	7	27	theme	MUC1	1100:1103	arg1	galactose/N-acetylgalactosamine					1052:1082	the terminal galactose/N-acetylgalactosamine	1039:1082	the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1	1039:1103	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
32502344	7	27	theme	MUC1	1100:1103	arg1	model					1110:1114	a model	1108:1114	a model	1108:1114	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
32502344	5	28	from	information	667:677	arg1	glycosylation					699:711	protein-specific glycosylation	682:711	protein-specific glycosylation	682:711	To collect biological information on protein-specific glycosylation and further explore clinical applications, quantitative detection of glycosylation is essential.					
32502344	4	29	theme	certain	525:531	arg1	O-glycans					533:541	certain O-glycans	525:541	certain O-glycans	525:541	Slightly aberrant expression of certain O-glycans can significantly affect cancer progression, especially at the cancer-related membrane protein level.					
32502344	10	30	theme	DNA	1344:1346	arg1	sequence					1348:1355	a DNA sequence	1342:1355	a DNA sequence complementary to that of the capture DNA	1342:1396	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	13	31	theme	peptide	1808:1814	arg1	response					1783:1790	The mass response	1774:1790	The mass response of the reporter peptide	1774:1814	The mass response of the reporter peptide represented the amount of MUC1-specific terminal Gal/GalNAc.					
32502344	8	32	theme	protein	1139:1145	arg1	probe					1147:1151	protein probe	1139:1151	protein probe	1139:1151	The dual-probe (i.e., protein probe and glycan probe) system was first designed and built.					
32502344	5	33	theme	biological	656:665	arg1	information					667:677	biological information	656:677	biological information on protein-specific glycosylation	656:711	To collect biological information on protein-specific glycosylation and further explore clinical applications, quantitative detection of glycosylation is essential.					
32502344	3	34	theme	types	461:465	arg1	types					461:465	cancer types	454:465	cancer types	454:465	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	3	34	theme	types	461:465	arg1	cancer					485:490	breast cancer	478:490	breast cancer	478:490	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	3	34	theme	types	461:465	arg1	variety					443:449	a variety	441:449	a variety of cancer types, including breast cancer	441:490	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	7	35	theme	terminal	1043:1050	arg1	Gal/GalNAc					1085:1094	Gal/GalNAc	1085:1094	Gal/GalNAc	1085:1094	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
32502344	7	35	theme	terminal	1043:1050	arg1	galactose/N-acetylgalactosamine					1052:1082	the terminal galactose/N-acetylgalactosamine	1039:1082	the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1	1039:1103	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
32502344	7	35	theme	terminal	1043:1050	arg1	model					1110:1114	a model	1108:1114	a model	1108:1114	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
32502344	10	36	theme	terminal	1519:1526	arg1	Gal/GalNAc					1528:1537	the terminal Gal/GalNAc	1515:1537	the terminal Gal/GalNAc	1515:1537	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	10	37	theme	complementary	1357:1369	arg1	sequence					1348:1355	a DNA sequence	1342:1355	a DNA sequence complementary to that of the capture DNA	1342:1396	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	1	38	theme	Protein	104:110	arg1	modification					160:171	a prevalent post-translational modification	129:171	a prevalent post-translational modification that mediates a variety of cellular processes	129:217	Protein glycosylation is a prevalent post-translational modification that mediates a variety of cellular processes.					
32502344	1	38	theme	Protein	104:110	arg1	glycosylation					112:124	Protein glycosylation	104:124	Protein glycosylation	104:124	Protein glycosylation is a prevalent post-translational modification that mediates a variety of cellular processes.					
32502344	12	39	theme	chromatography-tandem	1722:1742	arg1	LC-MS/MS					1763:1770	LC-MS/MS	1763:1770	LC-MS/MS	1763:1770	Finally, the reporter peptide was tryptically released and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32502344	12	39	theme	chromatography-tandem	1722:1742	arg1	spectrometry					1749:1760	liquid chromatography-tandem mass spectrometry	1715:1760	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1715:1771	Finally, the reporter peptide was tryptically released and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32502344	15	40	theme	cancer	2148:2153	arg1	diagnosis/prognosis					2155:2173	breast cancer diagnosis/prognosis	2141:2173	breast cancer diagnosis/prognosis	2141:2173	The relationship between MUC1-specific terminal Gal/GalNAc expression and breast cancer diagnosis/prognosis was also assessed.					
32502344	14	41	theme	in	1918:1919	arg1	detection					1926:1934	in situ detection	1918:1934	in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples	1918:2064	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	0	42	theme	Spectrometric	29:41	arg1	Quantification					43:56	Mass Spectrometric Quantification	24:56	Mass Spectrometric Quantification of MUC1-Specific Terminal Gal/GalNAc	24:93	Dual-Probe Approach for Mass Spectrometric Quantification of MUC1-Specific Terminal Gal/GalNAc In Situ.					
32502344	15	43	theme	Gal/GalNAc	2115:2124	arg1	expression					2126:2135	MUC1-specific terminal Gal/GalNAc expression	2092:2135	MUC1-specific terminal Gal/GalNAc expression	2092:2135	The relationship between MUC1-specific terminal Gal/GalNAc expression and breast cancer diagnosis/prognosis was also assessed.					
32502344	15	44	theme	MUC1-specific	2092:2104	arg1	expression					2126:2135	MUC1-specific terminal Gal/GalNAc expression	2092:2135	MUC1-specific terminal Gal/GalNAc expression	2092:2135	The relationship between MUC1-specific terminal Gal/GalNAc expression and breast cancer diagnosis/prognosis was also assessed.					
32502344	7	45	theme	glycosylation	1019:1031	arg1	quantification					984:997	mass spectrometric quantification	965:997	mass spectrometric quantification of protein-specific glycosylation	965:1031	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
32502344	14	46	theme	tissue	2051:2056	arg1	samples					2058:2064	matched breast cancer tissue samples	2029:2064	matched breast cancer tissue samples	2029:2064	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	14	47	theme	terminal	1953:1960	arg1	Gal/GalNAc					1962:1971	MUC1-specific terminal Gal/GalNAc	1939:1971	MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines	1939:2011	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	6	48	theme	few	819:821	arg1	assays					823:828	few assays	819:828	few assays	819:828	However, few assays have been reported for the in situ detection of protein-specific glycosylation to date.					
32502344	14	49	theme	breast	2037:2042	arg1	samples					2058:2064	matched breast cancer tissue samples	2029:2064	matched breast cancer tissue samples	2029:2064	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	9	50	theme	protein	1256:1262	arg1	recognition					1264:1274	MUC1 protein recognition	1251:1274	MUC1 protein recognition	1251:1274	The protein probe contained an aptamer for MUC1 protein recognition and a capture DNA sequence.					
32502344	7	51	theme	spectrometric	970:982	arg1	quantification					984:997	mass spectrometric quantification	965:997	mass spectrometric quantification of protein-specific glycosylation	965:1031	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
32502344	4	52	theme	membrane	621:628	arg1	level					638:642	the cancer-related membrane protein level	602:642	the cancer-related membrane protein level	602:642	Slightly aberrant expression of certain O-glycans can significantly affect cancer progression, especially at the cancer-related membrane protein level.					
32502344	8	53	dep	probe	1147:1151	arg1	i.e.					1133:1136	i.e.	1133:1136	i.e.	1133:1136	The dual-probe (i.e., protein probe and glycan probe) system was first designed and built.					
32502344	5	54	theme	clinical	733:740	arg1	applications					742:753	clinical applications	733:753	clinical applications	733:753	To collect biological information on protein-specific glycosylation and further explore clinical applications, quantitative detection of glycosylation is essential.					
32502344	6	55	theme	glycosylation	895:907	arg1	detection					865:873	the in situ detection	853:873	the in situ detection of protein-specific glycosylation to date	853:915	However, few assays have been reported for the in situ detection of protein-specific glycosylation to date.					
32502344	6	56	dep	in	857:858	arg1	situ					860:863	situ	860:863	situ	860:863	However, few assays have been reported for the in situ detection of protein-specific glycosylation to date.					
32502344	9	57	theme	capture	1282:1288	arg1	sequence					1294:1301	a capture DNA sequence	1280:1301	a capture DNA sequence	1280:1301	The protein probe contained an aptamer for MUC1 protein recognition and a capture DNA sequence.					
32502344	7	58	theme	dual-probe	941:950	arg1	approach					952:959	a dual-probe approach	939:959	a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation	939:1031	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
32502344	14	59	theme	matched	2029:2035	arg1	samples					2058:2064	matched breast cancer tissue samples	2029:2064	matched breast cancer tissue samples	2029:2064	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	13	60	theme	terminal	1856:1863	arg1	Gal/GalNAc					1865:1874	MUC1-specific terminal Gal/GalNAc	1842:1874	MUC1-specific terminal Gal/GalNAc	1842:1874	The mass response of the reporter peptide represented the amount of MUC1-specific terminal Gal/GalNAc.					
32502344	1	61	theme	processes	209:217	arg1	processes					209:217	cellular processes	200:217	cellular processes	200:217	Protein glycosylation is a prevalent post-translational modification that mediates a variety of cellular processes.					
32502344	1	61	theme	processes	209:217	arg1	variety					189:195	a variety	187:195	a variety of cellular processes	187:217	Protein glycosylation is a prevalent post-translational modification that mediates a variety of cellular processes.					
32502344	3	62	located	found	347:351	arg1	proteins					365:372	membrane proteins	356:372	membrane proteins	356:372	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	3	62	located	found	347:351	arg2	types					341:345	the various glycosylation types	315:345	the various glycosylation types found in membrane proteins	315:372	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	5	63	theme	glycosylation	782:794	arg1	detection					769:777	quantitative detection	756:777	quantitative detection of glycosylation	756:794	To collect biological information on protein-specific glycosylation and further explore clinical applications, quantitative detection of glycosylation is essential.					
32502344	2	64	theme	terminal	266:273	arg1	motif					275:279	their terminal motif	260:279	their terminal motif	260:279	For membrane proteins, glycosylation at their terminal motif is usually more functional.					
32502344	14	65	from	lines	2007:2011	arg1	detection					1926:1934	in situ detection	1918:1934	in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples	1918:2064	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	14	66	theme	cell	2002:2005	arg1	lines					2007:2011	three human breast cancer cell lines	1976:2011	three human breast cancer cell lines	1976:2011	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	2	67	gly	glycosylation	243:255	arg2	motif					275:279	their terminal motif	260:279	their terminal motif	260:279	For membrane proteins, glycosylation at their terminal motif is usually more functional.					
32502344	2	67	gly	glycosylation	243:255	arg1	motif					275:279	their terminal motif	260:279	their terminal motif	260:279	For membrane proteins, glycosylation at their terminal motif is usually more functional.					
32502344	14	68	theme	breast	1988:1993	arg1	lines					2007:2011	three human breast cancer cell lines	1976:2011	three human breast cancer cell lines	1976:2011	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	1	69	theme	post-translational	141:158	arg1	modification					160:171	a prevalent post-translational modification	129:171	a prevalent post-translational modification that mediates a variety of cellular processes	129:217	Protein glycosylation is a prevalent post-translational modification that mediates a variety of cellular processes.					
32502344	1	69	theme	post-translational	141:158	arg1	glycosylation					112:124	Protein glycosylation	104:124	Protein glycosylation	104:124	Protein glycosylation is a prevalent post-translational modification that mediates a variety of cellular processes.					
32502344	3	70	theme	glycosylation	327:339	arg1	types					341:345	the various glycosylation types	315:345	the various glycosylation types found in membrane proteins	315:372	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	0	71	theme	Gal/GalNAc	84:93	arg1	Quantification					43:56	Mass Spectrometric Quantification	24:56	Mass Spectrometric Quantification of MUC1-Specific Terminal Gal/GalNAc	24:93	Dual-Probe Approach for Mass Spectrometric Quantification of MUC1-Specific Terminal Gal/GalNAc In Situ.					
32502344	14	72	theme	Gal/GalNAc	1962:1971	arg1	detection					1926:1934	in situ detection	1918:1934	in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples	1918:2064	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	4	73	theme	O-glycans	533:541	arg1	expression					511:520	Slightly aberrant expression	493:520	Slightly aberrant expression of certain O-glycans	493:541	Slightly aberrant expression of certain O-glycans can significantly affect cancer progression, especially at the cancer-related membrane protein level.					
32502344	11	74	theme	hybridization-dehybridization	1581:1609	arg1	process					1611:1617	the hybridization-dehybridization process	1577:1617	the hybridization-dehybridization process	1577:1617	Exonuclease III enabled recycling of the hybridization-dehybridization process in a restricted space.					
32502344	14	75	theme	MUC1-specific	1939:1951	arg1	Gal/GalNAc					1962:1971	MUC1-specific terminal Gal/GalNAc	1939:1971	MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines	1939:2011	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	3	76	theme	membrane	356:363	arg1	proteins					365:372	membrane proteins	356:372	membrane proteins	356:372	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	13	77	theme	reporter	1799:1806	arg1	peptide					1808:1814	the reporter peptide	1795:1814	the reporter peptide	1795:1814	The mass response of the reporter peptide represented the amount of MUC1-specific terminal Gal/GalNAc.					
32502344	4	78	theme	aberrant	502:509	arg1	expression					511:520	Slightly aberrant expression	493:520	Slightly aberrant expression of certain O-glycans	493:541	Slightly aberrant expression of certain O-glycans can significantly affect cancer progression, especially at the cancer-related membrane protein level.					
32502344	8	79	theme	glycan	1157:1162	arg1	probe					1164:1168	glycan probe	1157:1168	glycan probe	1157:1168	The dual-probe (i.e., protein probe and glycan probe) system was first designed and built.					
32502344	14	80	theme	pairs	2020:2024	arg1	detection					1926:1934	in situ detection	1918:1934	in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples	1918:2064	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	13	81	theme	mass	1778:1781	arg1	response					1783:1790	The mass response	1774:1790	The mass response of the reporter peptide	1774:1814	The mass response of the reporter peptide represented the amount of MUC1-specific terminal Gal/GalNAc.					
32502344	3	82	theme	cancer	454:459	arg1	types					461:465	cancer types	454:465	cancer types	454:465	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	3	82	theme	cancer	454:459	arg1	cancer					485:490	breast cancer	478:490	breast cancer	478:490	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	0	83	theme	Mass	24:27	arg1	Quantification					43:56	Mass Spectrometric Quantification	24:56	Mass Spectrometric Quantification of MUC1-Specific Terminal Gal/GalNAc	24:93	Dual-Probe Approach for Mass Spectrometric Quantification of MUC1-Specific Terminal Gal/GalNAc In Situ.					
32502344	9	84	contain	contained	1226:1234	arg1	probe					1220:1224	The protein probe	1208:1224	The protein probe	1208:1224	The protein probe contained an aptamer for MUC1 protein recognition and a capture DNA sequence.					
32502344	9	84	contain	contained	1226:1234	arg2	aptamer					1239:1245	an aptamer	1236:1245	an aptamer for MUC1 protein recognition and a capture DNA sequence	1236:1301	The protein probe contained an aptamer for MUC1 protein recognition and a capture DNA sequence.					
32502344	2	85	from	motif	275:279	arg1	glycosylation					243:255	glycosylation	243:255	glycosylation at their terminal motif	243:279	For membrane proteins, glycosylation at their terminal motif is usually more functional.					
32502344	4	86	theme	cancer-related	606:619	arg1	level					638:642	the cancer-related membrane protein level	602:642	the cancer-related membrane protein level	602:642	Slightly aberrant expression of certain O-glycans can significantly affect cancer progression, especially at the cancer-related membrane protein level.					
32502344	10	87	theme	capture	1386:1392	arg1	DNA					1394:1396	the capture DNA	1382:1396	the capture DNA	1382:1396	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	10	88	contain	containing	1419:1428	arg1	peptide					1411:1417	a substrate peptide	1399:1417	a substrate peptide containing a reporter peptide	1399:1447	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	10	88	contain	containing	1419:1428	arg2	peptide					1441:1447	a reporter peptide	1430:1447	a reporter peptide	1430:1447	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	12	89	theme	mass	1744:1747	arg1	LC-MS/MS					1763:1770	LC-MS/MS	1763:1770	LC-MS/MS	1763:1770	Finally, the reporter peptide was tryptically released and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32502344	12	89	theme	mass	1744:1747	arg1	spectrometry					1749:1760	liquid chromatography-tandem mass spectrometry	1715:1760	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1715:1771	Finally, the reporter peptide was tryptically released and quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
32502344	4	90	theme	cancer	568:573	arg1	progression					575:585	cancer progression	568:585	cancer progression	568:585	Slightly aberrant expression of certain O-glycans can significantly affect cancer progression, especially at the cancer-related membrane protein level.					
32502344	15	91	theme	breast	2141:2146	arg1	diagnosis/prognosis					2155:2173	breast cancer diagnosis/prognosis	2141:2173	breast cancer diagnosis/prognosis	2141:2173	The relationship between MUC1-specific terminal Gal/GalNAc expression and breast cancer diagnosis/prognosis was also assessed.					
32502344	9	92	theme	protein	1212:1218	arg1	probe					1220:1224	The protein probe	1208:1224	The protein probe	1208:1224	The protein probe contained an aptamer for MUC1 protein recognition and a capture DNA sequence.					
32502344	3	93	theme	breast	478:483	arg1	cancer					485:490	breast cancer	478:490	breast cancer	478:490	Among the various glycosylation types found in membrane proteins, O-glycosylation is the most common and is closely correlated with a variety of cancer types, including breast cancer.					
32502344	0	94	theme	MUC1-Specific	61:73	arg1	Gal/GalNAc					84:93	MUC1-Specific Terminal Gal/GalNAc	61:93	MUC1-Specific Terminal Gal/GalNAc	61:93	Dual-Probe Approach for Mass Spectrometric Quantification of MUC1-Specific Terminal Gal/GalNAc In Situ.					
32502344	10	95	theme	substrate	1401:1409	arg1	peptide					1411:1417	a substrate peptide	1399:1417	a substrate peptide containing a reporter peptide	1399:1447	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	7	96	theme	protein-specific	1002:1017	arg1	glycosylation					1019:1031	protein-specific glycosylation	1002:1031	protein-specific glycosylation	1002:1031	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
32502344	14	97	theme	dual-probe	1882:1891	arg1	approach					1893:1900	This dual-probe approach	1877:1900	This dual-probe approach	1877:1900	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	14	98	theme	samples	2058:2064	arg1	pairs					2020:2024	32 pairs	2017:2024	32 pairs of matched breast cancer tissue samples	2017:2064	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	14	98	theme	samples	2058:2064	arg1	Gal/GalNAc					1962:1971	MUC1-specific terminal Gal/GalNAc	1939:1971	MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines	1939:2011	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	9	99	theme	MUC1	1251:1254	arg1	recognition					1264:1274	MUC1 protein recognition	1251:1274	MUC1 protein recognition	1251:1274	The protein probe contained an aptamer for MUC1 protein recognition and a capture DNA sequence.					
32502344	15	100	theme	terminal	2106:2113	arg1	expression					2126:2135	MUC1-specific terminal Gal/GalNAc expression	2092:2135	MUC1-specific terminal Gal/GalNAc expression	2092:2135	The relationship between MUC1-specific terminal Gal/GalNAc expression and breast cancer diagnosis/prognosis was also assessed.					
32502344	2	101	theme	membrane	224:231	arg1	proteins					233:240	membrane proteins	224:240	membrane proteins	224:240	For membrane proteins, glycosylation at their terminal motif is usually more functional.					
32502344	14	102	theme	cancer	2044:2049	arg1	samples					2058:2064	matched breast cancer tissue samples	2029:2064	matched breast cancer tissue samples	2029:2064	This dual-probe approach was applied for in situ detection of MUC1-specific terminal Gal/GalNAc in three human breast cancer cell lines and 32 pairs of matched breast cancer tissue samples.					
32502344	10	103	theme	reporter	1432:1439	arg1	peptide					1441:1447	a reporter peptide	1430:1447	a reporter peptide	1430:1447	Correspondingly, the glycan probe had a DNA sequence complementary to that of the capture DNA, a substrate peptide containing a reporter peptide, and a tryptic cleavage site, and could be covalently linked with the terminal Gal/GalNAc.					
32502344	7	104	theme	mass	965:968	arg1	quantification					984:997	mass spectrometric quantification	965:997	mass spectrometric quantification of protein-specific glycosylation	965:1031	Herein, we developed a dual-probe approach for mass spectrometric quantification of protein-specific glycosylation using the terminal galactose/N-acetylgalactosamine (Gal/GalNAc) of MUC1 as a model.					
31959207	4	0	theme	cell	822:825	arg1	line					827:830	the HEK293 cell line	811:830	the HEK293 cell line	811:830	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	8	1	theme	purified	1592:1599	arg1	protein					1601:1607	The purified protein	1588:1607	The purified protein	1588:1607	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	9	2	theme	polypeptide	2058:2068	arg1	chain					2070:2074	the polypeptide chain	2054:2074	the polypeptide chain	2054:2074	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	9	3	theme	chain	2070:2074	arg1	Asn137					2044:2049	residue Asn137	2036:2049	residue Asn137 of the polypeptide chain	2036:2074	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	1	4	theme	cell	243:246	arg1	proliferation					248:260	cell proliferation	243:260	cell proliferation	243:260	BACKGROUND The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway.					
31959207	7	5	theme	purification	1385:1396	arg1	strategy					1398:1405	a novel purification strategy	1377:1405	a novel purification strategy	1377:1405	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	11	6	from	cells	2351:2355	arg1	production					2289:2298	production	2289:2298	production	2289:2298	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	11	6	from	cells	2351:2355	arg1	purification					2304:2315	purification	2304:2315	purification	2304:2315	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	6	7	contain	containing	1114:1123	arg2	sequence					1143:1150	the hRSPO1 coding sequence	1125:1150	the hRSPO1 coding sequence	1125:1150	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	6	7	contain	containing	1114:1123	arg2	plasmid					1180:1186	a hygromycin resistance plasmid	1156:1186	a hygromycin resistance plasmid	1156:1186	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	6	7	contain	containing	1114:1123	arg1	vector					1107:1112	the recombinant expression vector	1080:1112	the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation	1080:1246	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	6	8	theme	clones	1231:1236	arg1	isolation					1238:1246	cell clones isolation	1226:1246	cell clones isolation	1226:1246	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	7	9	theme	sequential	1422:1431	arg1	steps					1449:1453	two sequential chromatographic steps	1418:1453	two sequential chromatographic steps	1418:1453	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	6	10	theme	coding	1136:1141	arg1	sequence					1143:1150	the hRSPO1 coding sequence	1125:1150	the hRSPO1 coding sequence	1125:1150	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	9	11	theme	rhRSPO1	1858:1864	arg1	analysis					1880:1887	rhRSPO1 glycosylation analysis	1858:1887	rhRSPO1 glycosylation analysis	1858:1887	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	8	12	theme	expression	1826:1835	arg1	system					1837:1842	this human cell expression system	1810:1842	this human cell expression system	1810:1842	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	8	13	theme	C2C12	1680:1684	arg1	cells					1686:1690	C2C12 cells	1680:1690	C2C12 cells	1680:1690	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	11	14	theme	recombinant	2320:2330	arg1	hRSPO1					2332:2337	recombinant hRSPO1	2320:2337	recombinant hRSPO1	2320:2337	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	11	15	theme	HEK293	2344:2349	arg1	cells					2351:2355	HEK293 cells	2344:2355	HEK293 cells	2344:2355	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	2	16	theme	stem	544:547	arg1	cells					549:553	intestinal stem cells	533:553	intestinal stem cells	533:553	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	7	17	theme	cells	1352:1356	arg1	supernatants					1317:1328	culture supernatants	1309:1328	culture supernatants of transfected HEK293 cells	1309:1356	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	0	18	theme	human	114:118	arg1	cells					127:131	human HEK293 cells	114:131	human HEK293 cells	114:131	Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.					
31959207	5	19	theme	cell	1011:1014	arg1	vector					1027:1032	a mammalian cell expression vector	999:1032	a mammalian cell expression vector	999:1032	The hRSPO1 coding sequence was synthesized and subcloned into a mammalian cell expression vector.					
31959207	8	20	theme	in	1697:1698	arg1	mice					1713:1716	BALB/c mice	1706:1716	BALB/c mice	1706:1716	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	8	20	theme	in	1697:1698	arg1	assays					1739:1744	in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays	1670:1744	in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays	1670:1744	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	1	21	theme	proteins	198:205	arg1	family					179:184	a family	177:184	a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death,	177:288	BACKGROUND The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway.					
31959207	4	22	theme	active	886:891	arg1	product					901:907	a purified, characterized and biologically active protein product	843:907	a purified, characterized and biologically active protein product to be used in Cell Therapy	843:934	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	7	23	theme	transfected	1333:1343	arg1	cells					1352:1356	transfected HEK293 cells	1333:1356	transfected HEK293 cells	1333:1356	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	3	24	theme	stem	738:741	arg1	cells					743:747	stem cells	738:747	stem cells	738:747	RSPO1 stands out among RSPOs molecules with respect to its potential therapeutic use, especially in the Regenerative Medicine field, due to its mitogenic activity in stem cells.					
31959207	1	25	from	roles	234:238	arg1	death					283:287	death	283:287	death	283:287	BACKGROUND The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway.					
31959207	1	25	from	roles	234:238	arg1	differentiation					263:277	differentiation	263:277	differentiation	263:277	BACKGROUND The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway.					
31959207	1	25	from	roles	234:238	arg1	proliferation					248:260	cell proliferation	243:260	cell proliferation	243:260	BACKGROUND The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway.					
31959207	7	26	theme	purity	1572:1577	arg1	product					1579:1585	a high purity product	1565:1585	a high purity product	1565:1585	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	9	27	theme	first	1921:1925	arg1	time					1927:1930	the first time	1917:1930	the first time	1917:1930	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	8	28	theme	biological	1719:1728	arg1	mice					1713:1716	BALB/c mice	1706:1716	BALB/c mice	1706:1716	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	8	28	theme	biological	1719:1728	arg1	assays					1739:1744	in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays	1670:1744	in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays	1670:1744	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	7	29	theme	serum	1297:1301	arg1	absence					1286:1292	the absence	1282:1292	the absence of serum	1282:1301	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	11	30	theme	purified	2403:2410	arg1	product					2465:2471	a purified, fully characterized and biologically active protein product	2401:2471	a purified, fully characterized and biologically active protein product	2401:2471	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	0	31	theme	R-spondin1	67:76	arg1	protein					86:92	recombinant human R-spondin1 (RSPO1) protein	49:92	recombinant human R-spondin1 (RSPO1) protein	49:92	Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.					
31959207	3	32	theme	RSPOs	595:599	arg1	molecules					601:609	RSPOs molecules	595:609	RSPOs molecules	595:609	RSPO1 stands out among RSPOs molecules with respect to its potential therapeutic use, especially in the Regenerative Medicine field, due to its mitogenic activity in stem cells.					
31959207	2	33	theme	muscle	466:471	arg1	development					480:490	skeletal muscle tissue development	457:490	skeletal muscle tissue development	457:490	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	33	theme	muscle	466:471	arg1	processes					422:430	tissue-specific processes	406:430	tissue-specific processes	406:430	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	34	theme	cells	549:553	arg1	proliferation					493:505	proliferation	493:505	proliferation of pancreatic β-cells and intestinal stem cells	493:553	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	34	theme	cells	549:553	arg1	development					480:490	skeletal muscle tissue development	457:490	skeletal muscle tissue development	457:490	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	34	theme	cells	549:553	arg1	formation					446:454	bone formation	441:454	bone formation	441:454	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	34	theme	cells	549:553	arg1	processes					422:430	tissue-specific processes	406:430	tissue-specific processes	406:430	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	34	theme	cells	549:553	arg1	cancer					564:569	even cancer	559:569	even cancer	559:569	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	0	35	theme	RSPO1	79:83	arg1	protein					86:92	recombinant human R-spondin1 (RSPO1) protein	49:92	recombinant human R-spondin1 (RSPO1) protein	49:92	Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.					
31959207	8	36	theme	human	1815:1819	arg1	system					1837:1842	this human cell expression system	1810:1842	this human cell expression system	1810:1842	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	2	37	theme	pancreatic	510:519	arg1	β-cells					521:527	pancreatic β-cells	510:527	pancreatic β-cells	510:527	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	6	38	theme	resistance	1169:1178	arg1	plasmid					1180:1186	a hygromycin resistance plasmid	1156:1186	a hygromycin resistance plasmid	1156:1186	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	10	39	theme	structures	2162:2171	arg1	presence					2143:2150	the presence	2139:2150	the presence of glycan structures such as terminal sialic acid, N-acetylglucosamine and/or galactose	2139:2238	In addition, this analysis revealing the presence of glycan structures such as terminal sialic acid, N-acetylglucosamine and/or galactose.					
31959207	0	40	theme	protein	86:92	arg1	purification					12:23	purification	12:23	purification	12:23	Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.					
31959207	0	40	theme	protein	86:92	arg1	Production					0:9	Production	0:9	Production	0:9	Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.					
31959207	0	40	theme	protein	86:92	arg1	characterization					29:44	characterization	29:44	characterization	29:44	Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.					
31959207	8	41	theme	structural	1762:1771	arg1	integrity					1773:1781	the structural integrity	1758:1781	the structural integrity	1758:1781	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	7	42	theme	exclusion	1521:1529	arg1	chromatography					1531:1544	a molecular exclusion chromatography	1509:1544	a molecular exclusion chromatography	1509:1544	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	11	43	theme	product	2465:2471	arg1	production					2387:2396	the production	2383:2396	the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering	2383:2507	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	9	44	theme	oligosaccharide	1964:1978	arg1	chain					1980:1984	this oligosaccharide chain	1959:1984	this oligosaccharide chain	1959:1984	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	11	45	theme	active	2450:2455	arg1	product					2465:2471	a purified, fully characterized and biologically active protein product	2401:2471	a purified, fully characterized and biologically active protein product	2401:2471	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	3	46	from	activity	726:733	arg1	cells					743:747	stem cells	738:747	stem cells	738:747	RSPO1 stands out among RSPOs molecules with respect to its potential therapeutic use, especially in the Regenerative Medicine field, due to its mitogenic activity in stem cells.					
31959207	10	47	theme	sialic	2190:2195	arg1	acid					2197:2200	terminal sialic acid	2181:2200	terminal sialic acid	2181:2200	In addition, this analysis revealing the presence of glycan structures such as terminal sialic acid, N-acetylglucosamine and/or galactose.					
31959207	2	48	theme	RSPOs	371:375	arg1	importance					357:366	the importance	353:366	the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer	353:569	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	8	49	theme	system	1837:1842	arg1	integrity					1773:1781	the structural integrity	1758:1781	the structural integrity	1758:1781	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	8	49	theme	system	1837:1842	arg1	efficacy					1798:1805	biological efficacy	1787:1805	biological efficacy	1787:1805	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	2	50	theme	processes	422:430	arg1	number					396:401	a number	394:401	a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer	394:569	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	6	51	theme	hygromycin	1158:1167	arg1	plasmid					1180:1186	a hygromycin resistance plasmid	1156:1186	a hygromycin resistance plasmid	1156:1186	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	8	52	dep	in	1670:1671	arg1	vitro					1673:1677	vitro	1673:1677	vitro	1673:1677	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	8	52	dep	in	1670:1671	arg1	cells					1686:1690	C2C12 cells	1680:1690	C2C12 cells	1680:1690	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	1	53	dep	BACKGROUND	134:143	arg1	comprise					168:175	comprise	168:175	comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway	168:316	BACKGROUND The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway.					
31959207	3	54	theme	potential	631:639	arg1	use					653:655	its potential therapeutic use	627:655	its potential therapeutic use	627:655	RSPO1 stands out among RSPOs molecules with respect to its potential therapeutic use, especially in the Regenerative Medicine field, due to its mitogenic activity in stem cells.					
31959207	4	55	theme	human	783:787	arg1	rhRSPO1					796:802	rhRSPO1	796:802	rhRSPO1	796:802	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	4	55	theme	human	783:787	arg1	RSPO1					789:793	a recombinant human RSPO1	769:793	a recombinant human RSPO1 (rhRSPO1)	769:803	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	2	56	theme	bone	441:444	arg1	formation					446:454	bone formation	441:454	bone formation	441:454	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	56	theme	bone	441:444	arg1	processes					422:430	tissue-specific processes	406:430	tissue-specific processes	406:430	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	9	57	theme	N-glycosylation	2017:2031	arg1	presence					2002:2009	the presence	1998:2009	the presence of an N-glycosylation in residue Asn137 of the polypeptide chain	1998:2074	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	0	58	theme	recombinant	49:59	arg1	protein					86:92	recombinant human R-spondin1 (RSPO1) protein	49:92	recombinant human R-spondin1 (RSPO1) protein	49:92	Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.					
31959207	7	59	theme	affinity	1472:1479	arg1	chromatography					1481:1494	heparin affinity chromatography	1464:1494	heparin affinity chromatography	1464:1494	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	9	60	theme	residue	2036:2042	arg1	Asn137					2044:2049	residue Asn137	2036:2049	residue Asn137 of the polypeptide chain	2036:2074	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	6	61	theme	hRSPO1	1129:1134	arg1	sequence					1143:1150	the hRSPO1 coding sequence	1125:1150	the hRSPO1 coding sequence	1125:1150	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	11	62	dep	CONCLUSION	2241:2250	arg1	generated					2361:2369	generated	2361:2369	generated	2361:2369	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	8	63	theme	Western	1630:1636	arg1	blotting					1638:1645	Western blotting	1630:1645	Western blotting	1630:1645	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	1	64	theme	important	224:232	arg1	roles					234:238	their important roles	218:238	their important roles in cell proliferation, differentiation and death	218:287	BACKGROUND The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway.					
31959207	5	65	theme	coding	948:953	arg1	sequence					955:962	The hRSPO1 coding sequence	937:962	The hRSPO1 coding sequence	937:962	The hRSPO1 coding sequence was synthesized and subcloned into a mammalian cell expression vector.					
31959207	3	66	theme	Medicine	689:696	arg1	field					698:702	the Regenerative Medicine field	672:702	the Regenerative Medicine field	672:702	RSPO1 stands out among RSPOs molecules with respect to its potential therapeutic use, especially in the Regenerative Medicine field, due to its mitogenic activity in stem cells.					
31959207	7	67	theme	chromatographic	1433:1447	arg1	steps					1449:1453	two sequential chromatographic steps	1418:1453	two sequential chromatographic steps	1418:1453	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	4	68	theme	HEK293	815:820	arg1	line					827:830	the HEK293 cell line	811:830	the HEK293 cell line	811:830	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	6	69	theme	HEK293	1035:1040	arg1	cells					1042:1046	HEK293 cells	1035:1046	HEK293 cells	1035:1046	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	4	70	theme	characterized	855:867	arg1	product					901:907	a purified, characterized and biologically active protein product	843:907	a purified, characterized and biologically active protein product to be used in Cell Therapy	843:934	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	8	71	theme	cell	1821:1824	arg1	system					1837:1842	this human cell expression system	1810:1842	this human cell expression system	1810:1842	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	9	72	theme	glycan	1937:1942	arg1	composition					1944:1954	the glycan composition	1933:1954	the glycan composition of this oligosaccharide chain	1933:1984	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	6	73	theme	cell	1226:1229	arg1	clones					1231:1236	cell clones	1226:1236	cell clones isolation	1226:1246	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	8	74	theme	in	1670:1671	arg1	mice					1713:1716	BALB/c mice	1706:1716	BALB/c mice	1706:1716	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	8	74	theme	in	1670:1671	arg1	assays					1739:1744	in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays	1670:1744	in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays	1670:1744	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	7	75	theme	novel	1379:1383	arg1	strategy					1398:1405	a novel purification strategy	1377:1405	a novel purification strategy	1377:1405	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	9	76	theme	glycosylation	1866:1878	arg1	analysis					1880:1887	rhRSPO1 glycosylation analysis	1858:1887	rhRSPO1 glycosylation analysis	1858:1887	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	2	77	theme	skeletal	457:464	arg1	development					480:490	skeletal muscle tissue development	457:490	skeletal muscle tissue development	457:490	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	77	theme	skeletal	457:464	arg1	processes					422:430	tissue-specific processes	406:430	tissue-specific processes	406:430	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	11	78	dep	production	2289:2298	arg1	the					2285:2287	the	2285:2287	the	2285:2287	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	2	79	from	importance	357:366	arg1	regulation					380:389	regulation	380:389	regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer	380:569	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	0	80	theme	HEK293	120:125	arg1	cells					127:131	human HEK293 cells	114:131	human HEK293 cells	114:131	Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.					
31959207	11	81	theme	hRSPO1	2332:2337	arg1	production					2289:2298	production	2289:2298	production	2289:2298	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	11	81	theme	hRSPO1	2332:2337	arg1	purification					2304:2315	purification	2304:2315	purification	2304:2315	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	1	82	theme	secreted	189:196	arg1	proteins					198:205	secreted proteins	189:205	secreted proteins	189:205	BACKGROUND The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway.					
31959207	2	83	theme	intestinal	533:542	arg1	cells					549:553	intestinal stem cells	533:553	intestinal stem cells	533:553	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	6	84	theme	expression	1096:1105	arg1	vector					1107:1112	the recombinant expression vector	1080:1112	the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation	1080:1246	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	3	85	theme	mitogenic	716:724	arg1	activity					726:733	its mitogenic activity	712:733	its mitogenic activity in stem cells	712:747	RSPO1 stands out among RSPOs molecules with respect to its potential therapeutic use, especially in the Regenerative Medicine field, due to its mitogenic activity in stem cells.					
31959207	5	86	theme	expression	1016:1025	arg1	vector					1027:1032	a mammalian cell expression vector	999:1032	a mammalian cell expression vector	999:1032	The hRSPO1 coding sequence was synthesized and subcloned into a mammalian cell expression vector.					
31959207	2	87	theme	β-cells	521:527	arg1	proliferation					493:505	proliferation	493:505	proliferation of pancreatic β-cells and intestinal stem cells	493:553	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	87	theme	β-cells	521:527	arg1	development					480:490	skeletal muscle tissue development	457:490	skeletal muscle tissue development	457:490	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	87	theme	β-cells	521:527	arg1	formation					446:454	bone formation	441:454	bone formation	441:454	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	87	theme	β-cells	521:527	arg1	processes					422:430	tissue-specific processes	406:430	tissue-specific processes	406:430	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	87	theme	β-cells	521:527	arg1	cancer					564:569	even cancer	559:569	even cancer	559:569	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	7	88	theme	HEK293	1345:1350	arg1	cells					1352:1356	transfected HEK293 cells	1333:1356	transfected HEK293 cells	1333:1356	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	6	89	dep	sequence	1143:1150	arg1	selected					1189:1196	selected	1189:1196	selected for hygror	1189:1207	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	6	89	dep	sequence	1143:1150	arg1	subjected					1213:1221	subjected	1213:1221	subjected to cell clones isolation	1213:1246	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	7	90	theme	high	1567:1570	arg1	product					1579:1585	a high purity product	1565:1585	a high purity product	1565:1585	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	2	91	theme	even	559:562	arg1	processes					422:430	tissue-specific processes	406:430	tissue-specific processes	406:430	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	91	theme	even	559:562	arg1	cancer					564:569	even cancer	559:569	even cancer	559:569	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	8	92	theme	BALB/c	1706:1711	arg1	mice					1713:1716	BALB/c mice	1706:1716	BALB/c mice	1706:1716	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	8	92	theme	BALB/c	1706:1711	arg1	assays					1739:1744	in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays	1670:1744	in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays	1670:1744	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	4	93	theme	purified	845:852	arg1	product					901:907	a purified, characterized and biologically active protein product	843:907	a purified, characterized and biologically active protein product to be used in Cell Therapy	843:934	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	11	94	theme	Tissue	2490:2495	arg1	Engineering					2497:2507	Tissue Engineering	2490:2507	Tissue Engineering	2490:2507	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	7	95	theme	culture	1309:1315	arg1	supernatants					1317:1328	culture supernatants	1309:1328	culture supernatants of transfected HEK293 cells	1309:1356	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	9	96	from	Asn137	2044:2049	arg1	presence					2002:2009	the presence	1998:2009	the presence of an N-glycosylation in residue Asn137 of the polypeptide chain	1998:2074	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	5	97	theme	mammalian	1001:1009	arg1	vector					1027:1032	a mammalian cell expression vector	999:1032	a mammalian cell expression vector	999:1032	The hRSPO1 coding sequence was synthesized and subcloned into a mammalian cell expression vector.					
31959207	2	98	theme	tissue	473:478	arg1	development					480:490	skeletal muscle tissue development	457:490	skeletal muscle tissue development	457:490	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	98	theme	tissue	473:478	arg1	processes					422:430	tissue-specific processes	406:430	tissue-specific processes	406:430	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	11	99	theme	characterized	2419:2431	arg1	product					2465:2471	a purified, fully characterized and biologically active protein product	2401:2471	a purified, fully characterized and biologically active protein product	2401:2471	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	9	100	from	N-glycosylation	2017:2031	arg1	Asn137					2044:2049	residue Asn137	2036:2049	residue Asn137 of the polypeptide chain	2036:2074	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	8	101	theme	activity	1730:1737	arg1	mice					1713:1716	BALB/c mice	1706:1716	BALB/c mice	1706:1716	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	8	101	theme	activity	1730:1737	arg1	assays					1739:1744	in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays	1670:1744	in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays	1670:1744	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	1	102	theme	R-Spondin	149:157	arg1	proteins					159:166	The R-Spondin proteins	145:166	The R-Spondin proteins	145:166	BACKGROUND The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway.					
31959207	4	103	theme	Cell	923:926	arg1	Therapy					928:934	Cell Therapy	923:934	Cell Therapy	923:934	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	8	104	dep	in	1697:1698	arg1	vivo					1700:1703	vivo	1700:1703	vivo	1700:1703	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	7	105	theme	molecular	1511:1519	arg1	chromatography					1531:1544	a molecular exclusion chromatography	1509:1544	a molecular exclusion chromatography	1509:1544	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	4	106	theme	protein	893:899	arg1	product					901:907	a purified, characterized and biologically active protein product	843:907	a purified, characterized and biologically active protein product to be used in Cell Therapy	843:934	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	11	107	theme	protein	2457:2463	arg1	product					2465:2471	a purified, fully characterized and biologically active protein product	2401:2471	a purified, fully characterized and biologically active protein product	2401:2471	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	10	108	theme	glycan	2155:2160	arg1	structures					2162:2171	glycan structures	2155:2171	glycan structures such as terminal sialic acid, N-acetylglucosamine and/or galactose	2155:2238	In addition, this analysis revealing the presence of glycan structures such as terminal sialic acid, N-acetylglucosamine and/or galactose.					
31959207	10	108	theme	glycan	2155:2160	arg1	galactose					2230:2238	galactose	2230:2238	galactose	2230:2238	In addition, this analysis revealing the presence of glycan structures such as terminal sialic acid, N-acetylglucosamine and/or galactose.					
31959207	10	108	theme	glycan	2155:2160	arg1	N-acetylglucosamine					2203:2221	N-acetylglucosamine	2203:2221	N-acetylglucosamine	2203:2221	In addition, this analysis revealing the presence of glycan structures such as terminal sialic acid, N-acetylglucosamine and/or galactose.					
31959207	10	108	theme	glycan	2155:2160	arg1	acid					2197:2200	terminal sialic acid	2181:2200	terminal sialic acid	2181:2200	In addition, this analysis revealing the presence of glycan structures such as terminal sialic acid, N-acetylglucosamine and/or galactose.					
31959207	9	109	from	presence	2002:2009	arg1	Asn137					2044:2049	residue Asn137	2036:2049	residue Asn137 of the polypeptide chain	2036:2074	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	2	110	dep	processes	422:430	arg1	proliferation					493:505	proliferation	493:505	proliferation of pancreatic β-cells and intestinal stem cells	493:553	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	110	dep	processes	422:430	arg1	development					480:490	skeletal muscle tissue development	457:490	skeletal muscle tissue development	457:490	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	110	dep	processes	422:430	arg1	formation					446:454	bone formation	441:454	bone formation	441:454	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	110	dep	processes	422:430	arg1	processes					422:430	tissue-specific processes	406:430	tissue-specific processes	406:430	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	110	dep	processes	422:430	arg1	cancer					564:569	even cancer	559:569	even cancer	559:569	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	9	111	theme	chain	1980:1984	arg1	composition					1944:1954	the glycan composition	1933:1954	the glycan composition of this oligosaccharide chain	1933:1984	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	9	112	gly	N-glycosylation	2017:2031	arg1	Asn137					2044:2049	residue Asn137	2036:2049	residue Asn137 of the polypeptide chain	2036:2074	Furthermore, rhRSPO1 glycosylation analysis allowed us to describe, for the first time, the glycan composition of this oligosaccharide chain, confirming the presence of an N-glycosylation in residue Asn137 of the polypeptide chain, as previously described.					
31959207	10	113	theme	terminal	2181:2188	arg1	acid					2197:2200	terminal sialic acid	2181:2200	terminal sialic acid	2181:2200	In addition, this analysis revealing the presence of glycan structures such as terminal sialic acid, N-acetylglucosamine and/or galactose.					
31959207	7	114	dep	steps	1449:1453	arg1	chromatography					1481:1494	heparin affinity chromatography	1464:1494	heparin affinity chromatography	1464:1494	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	8	115	theme	biological	1787:1796	arg1	efficacy					1798:1805	biological efficacy	1787:1805	biological efficacy	1787:1805	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
31959207	2	116	theme	tissue-specific	406:420	arg1	proliferation					493:505	proliferation	493:505	proliferation of pancreatic β-cells and intestinal stem cells	493:553	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	116	theme	tissue-specific	406:420	arg1	development					480:490	skeletal muscle tissue development	457:490	skeletal muscle tissue development	457:490	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	116	theme	tissue-specific	406:420	arg1	formation					446:454	bone formation	441:454	bone formation	441:454	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	116	theme	tissue-specific	406:420	arg1	processes					422:430	tissue-specific processes	406:430	tissue-specific processes	406:430	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	116	theme	tissue-specific	406:420	arg1	cancer					564:569	even cancer	559:569	even cancer	559:569	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	2	117	theme	number	396:401	arg1	regulation					380:389	regulation	380:389	regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer	380:569	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	4	118	theme	recombinant	771:781	arg1	rhRSPO1					796:802	rhRSPO1	796:802	rhRSPO1	796:802	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	4	118	theme	recombinant	771:781	arg1	RSPO1					789:793	a recombinant human RSPO1	769:793	a recombinant human RSPO1 (rhRSPO1)	769:803	Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.					
31959207	0	119	theme	human	61:65	arg1	protein					86:92	recombinant human R-spondin1 (RSPO1) protein	49:92	recombinant human R-spondin1 (RSPO1) protein	49:92	Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.					
31959207	6	120	theme	recombinant	1084:1094	arg1	vector					1107:1112	the recombinant expression vector	1080:1112	the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation	1080:1246	HEK293 cells were stably co-transfected with the recombinant expression vector containing the hRSPO1 coding sequence and a hygromycin resistance plasmid, selected for hygror and subjected to cell clones isolation.					
31959207	2	121	theme	Several	319:325	arg1	studies					327:333	Several studies	319:333	Several studies	319:333	Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer.					
31959207	1	122	theme	Wnt	306:308	arg1	pathway					310:316	the Wnt pathway	302:316	the Wnt pathway	302:316	BACKGROUND The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway.					
31959207	3	123	theme	therapeutic	641:651	arg1	use					653:655	its potential therapeutic use	627:655	its potential therapeutic use	627:655	RSPO1 stands out among RSPOs molecules with respect to its potential therapeutic use, especially in the Regenerative Medicine field, due to its mitogenic activity in stem cells.					
31959207	7	124	theme	heparin	1464:1470	arg1	chromatography					1481:1494	heparin affinity chromatography	1464:1494	heparin affinity chromatography	1464:1494	RESULTS rhRSPO1 was obtained, in the absence of serum, from culture supernatants of transfected HEK293 cells and purified using a novel purification strategy, involving two sequential chromatographic steps, namely: heparin affinity chromatography, followed by a molecular exclusion chromatography, designed to yield a high purity product.					
31959207	5	125	theme	hRSPO1	941:946	arg1	sequence					955:962	The hRSPO1 coding sequence	937:962	The hRSPO1 coding sequence	937:962	The hRSPO1 coding sequence was synthesized and subcloned into a mammalian cell expression vector.					
31959207	11	126	theme	stable	2265:2270	arg1	platform					2272:2279	a stable platform	2263:2279	a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells	2263:2355	CONCLUSION Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering.					
31959207	3	127	theme	Regenerative	676:687	arg1	field					698:702	the Regenerative Medicine field	672:702	the Regenerative Medicine field	672:702	RSPO1 stands out among RSPOs molecules with respect to its potential therapeutic use, especially in the Regenerative Medicine field, due to its mitogenic activity in stem cells.					
31959207	8	128	theme	mass	1648:1651	arg1	spectrometry					1653:1664	mass spectrometry	1648:1664	mass spectrometry	1648:1664	The purified protein was characterized by Western blotting, mass spectrometry and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming the structural integrity and biological efficacy of this human cell expression system.					
33484712	4	0	theme	conformational	1058:1071	arg1	transitions					1073:1083	the large-scale conformational transitions	1042:1083	the large-scale conformational transitions	1042:1083	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	8	1	theme	functional	1714:1723	arg1	motions					1725:1731	functional motions	1714:1731	functional motions	1714:1731	This study sheds light on the mechanisms underlying conformational stability and functional motions of the S-protein, which are relevant for vaccine and antiviral drug developments.					
33484712	6	2	theme	glycosylated	1354:1365	arg1	residues					1367:1374	glycosylated residues	1354:1374	glycosylated residues	1354:1374	During the conformational transitions between them, interdomain interactions mediated by glycosylated residues are switched to play key roles on the stabilization of another form.					
33484712	5	3	theme	form	1259:1262	arg1	stability					1241:1249	conformational stability	1226:1249	conformational stability of each form	1226:1262	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	4	4	theme	S-protein	875:883	arg1	forms					845:849	both down and up forms	828:849	both down and up forms of a fully glycosylated S-protein in solution	828:895	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	3	5	theme	inactive	731:738	arg1	forms					759:763	the inactive down and active up forms	727:763	the inactive down and active up forms	727:763	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	3	6	theme	atomic	545:550	arg1	resolutions					552:562	atomic resolutions	545:562	atomic resolutions	545:562	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	8	7	theme	antiviral	1786:1794	arg1	developments					1801:1812	antiviral drug developments	1786:1812	antiviral drug developments	1786:1812	This study sheds light on the mechanisms underlying conformational stability and functional motions of the S-protein, which are relevant for vaccine and antiviral drug developments.					
33484712	6	8	theme	interdomain	1317:1327	arg1	interactions					1329:1340	interdomain interactions	1317:1340	interdomain interactions mediated by glycosylated residues	1317:1374	During the conformational transitions between them, interdomain interactions mediated by glycosylated residues are switched to play key roles on the stabilization of another form.					
33484712	3	9	theme	structure	532:540	arg1	understanding					501:513	advanced our understanding	488:513	advanced our understanding of the S-protein structure at atomic resolutions	488:562	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	7	10	theme	binding	1526:1532	arg1	domains					1534:1540	the three receptor binding domains	1507:1540	the three receptor binding domains	1507:1540	Electrostatic interactions, as well as hydrogen bonds between the three receptor binding domains, work as driving forces to initiate the conformational transitions toward the active form.					
33484712	3	11	theme	up	756:757	arg1	forms					759:763	the inactive down and active up forms	727:763	the inactive down and active up forms	727:763	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	2	12	theme	trimeric	301:308	arg1	chains					322:327	trimeric polypeptide chains	301:327	trimeric polypeptide chains with glycosylated residues on the surface	301:369	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	5	13	from	determinants	1210:1221	arg1	stability					1241:1249	conformational stability	1226:1249	conformational stability of each form	1226:1262	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	4	14	theme	targeted	908:915	arg1	simulations					936:946	targeted molecular dynamics simulations	908:946	all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them	785:959	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	5	15	theme	residue-level	1090:1102	arg1	analysis					1116:1123	The residue-level interaction analysis	1086:1123	The residue-level interaction analysis of the simulation trajectories	1086:1154	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	1	16	theme	vaccines	208:215	arg1	developments					192:203	urgent developments	185:203	urgent developments of vaccines and antiviral drugs	185:235	The ongoing COVID-19 pandemic caused by the new coronavirus, SARS-CoV-2, calls for urgent developments of vaccines and antiviral drugs.					
33484712	4	17	theme	dynamics	927:934	arg1	simulations					936:946	targeted molecular dynamics simulations	908:946	all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them	785:959	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	6	18	theme	key	1397:1399	arg1	roles					1401:1405	key roles	1397:1405	key roles	1397:1405	During the conformational transitions between them, interdomain interactions mediated by glycosylated residues are switched to play key roles on the stabilization of another form.					
33484712	1	19	theme	antiviral	221:229	arg1	drugs					231:235	antiviral drugs	221:235	antiviral drugs	221:235	The ongoing COVID-19 pandemic caused by the new coronavirus, SARS-CoV-2, calls for urgent developments of vaccines and antiviral drugs.					
33484712	3	20	attach	attached	638:645	arg2	glycans					630:636	glycans	630:636	glycans attached to the S-protein	630:662	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	3	20	attach	attached	638:645	arg1	S-protein					654:662	the S-protein	650:662	the S-protein	650:662	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	6	21	theme	conformational	1276:1289	arg1	transitions					1291:1301	the conformational transitions	1272:1301	the conformational transitions between them	1272:1314	During the conformational transitions between them, interdomain interactions mediated by glycosylated residues are switched to play key roles on the stabilization of another form.					
33484712	4	22	from	solution	888:895	arg1	forms					845:849	both down and up forms	828:849	both down and up forms of a fully glycosylated S-protein in solution	828:895	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	2	23	theme	SARS-CoV-2	259:268	arg1	S-protein					271:279	S-protein	271:279	S-protein	271:279	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	2	23	theme	SARS-CoV-2	259:268	arg1	protein					248:254	The spike protein	238:254	The spike protein of SARS-CoV-2 (S-protein)	238:280	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	1	24	theme	new	146:148	arg1	calls					175:179	calls	175:179	calls for urgent developments of vaccines and antiviral drugs	175:235	The ongoing COVID-19 pandemic caused by the new coronavirus, SARS-CoV-2, calls for urgent developments of vaccines and antiviral drugs.					
33484712	1	24	theme	new	146:148	arg1	SARS-CoV-2					163:172	SARS-CoV-2	163:172	SARS-CoV-2	163:172	The ongoing COVID-19 pandemic caused by the new coronavirus, SARS-CoV-2, calls for urgent developments of vaccines and antiviral drugs.					
33484712	1	24	theme	new	146:148	arg1	coronavirus					150:160	the new coronavirus	142:160	the new coronavirus	142:160	The ongoing COVID-19 pandemic caused by the new coronavirus, SARS-CoV-2, calls for urgent developments of vaccines and antiviral drugs.					
33484712	4	25	theme	key	974:976	arg1	interactions					990:1001	key interdomain interactions	974:1001	key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions	974:1083	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	5	26	theme	distinct	1164:1171	arg1	determinants					1210:1221	determinants	1210:1221	determinants on conformational stability of each form	1210:1262	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	5	26	theme	distinct	1164:1171	arg1	N-glycans					1197:1205	N-glycans	1197:1205	N-glycans	1197:1205	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	5	26	theme	distinct	1164:1171	arg1	residues					1184:1191	distinct amino acid residues	1164:1191	distinct amino acid residues	1164:1191	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	0	27	theme	S-protein	91:99	arg1	stability					54:62	conformational stability	39:62	conformational stability	39:62	Elucidation of interactions regulating conformational stability and dynamics of SARS-CoV-2 S-protein.					
33484712	0	27	theme	S-protein	91:99	arg1	dynamics					68:75	dynamics	68:75	dynamics	68:75	Elucidation of interactions regulating conformational stability and dynamics of SARS-CoV-2 S-protein.					
33484712	5	28	theme	acid	1179:1182	arg1	determinants					1210:1221	determinants	1210:1221	determinants on conformational stability of each form	1210:1262	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	5	28	theme	acid	1179:1182	arg1	N-glycans					1197:1205	N-glycans	1197:1205	N-glycans	1197:1205	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	5	28	theme	acid	1179:1182	arg1	residues					1184:1191	distinct amino acid residues	1164:1191	distinct amino acid residues	1164:1191	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	3	29	theme	structural	425:434	arg1	studies					451:457	Extensive structural and functional studies	415:457	Extensive structural and functional studies on this protein	415:473	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	5	30	theme	trajectories	1143:1154	arg1	analysis					1116:1123	The residue-level interaction analysis	1086:1123	The residue-level interaction analysis of the simulation trajectories	1086:1154	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	4	31	dep	forms	845:849	arg1	both					828:831	both	828:831	both	828:831	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	4	31	dep	forms	845:849	arg1	up					842:843	up	842:843	up	842:843	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	3	32	dep	stability	686:694	arg1	the					667:669	the	667:669	the	667:669	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	1	33	theme	ongoing	106:112	arg1	pandemic					123:130	The ongoing COVID-19 pandemic	102:130	The ongoing COVID-19 pandemic	102:130	The ongoing COVID-19 pandemic caused by the new coronavirus, SARS-CoV-2, calls for urgent developments of vaccines and antiviral drugs.					
33484712	2	34	with	chains	322:327	arg1	residues					347:354	glycosylated residues	334:354	glycosylated residues on the surface	334:369	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	4	35	theme	large-scale	1046:1056	arg1	transitions					1073:1083	the large-scale conformational transitions	1042:1083	the large-scale conformational transitions	1042:1083	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	0	36	theme	interactions	15:26	arg1	Elucidation					0:10	Elucidation	0:10	Elucidation of interactions	0:26	Elucidation of interactions regulating conformational stability and dynamics of SARS-CoV-2 S-protein.					
33484712	3	37	theme	functional	440:449	arg1	studies					451:457	Extensive structural and functional studies	415:457	Extensive structural and functional studies on this protein	415:473	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	0	38	theme	conformational	39:52	arg1	stability					54:62	conformational stability	39:62	conformational stability	39:62	Elucidation of interactions regulating conformational stability and dynamics of SARS-CoV-2 S-protein.					
33484712	2	39	gly	glycosylated	334:345	arg1	residues					347:354	glycosylated residues	334:354	glycosylated residues on the surface	334:369	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	3	40	from	studies	451:457	arg1	protein					467:473	this protein	462:473	this protein	462:473	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	4	41	from	S-protein	875:883	arg1	solution					888:895	solution	888:895	solution	888:895	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	3	42	theme	conformational	671:684	arg1	stability					686:694	conformational stability	671:694	conformational stability	671:694	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	4	43	theme	all-atom	785:792	arg1	dynamics					804:811	all-atom molecular dynamics	785:811	all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them	785:959	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	1	44	theme	drugs	231:235	arg1	developments					192:203	urgent developments	185:203	urgent developments of vaccines and antiviral drugs	185:235	The ongoing COVID-19 pandemic caused by the new coronavirus, SARS-CoV-2, calls for urgent developments of vaccines and antiviral drugs.					
33484712	7	45	theme	driving	1551:1557	arg1	forces					1559:1564	driving forces	1551:1564	driving forces	1551:1564	Electrostatic interactions, as well as hydrogen bonds between the three receptor binding domains, work as driving forces to initiate the conformational transitions toward the active form.					
33484712	8	46	theme	conformational	1685:1698	arg1	stability					1700:1708	conformational stability	1685:1708	conformational stability	1685:1708	This study sheds light on the mechanisms underlying conformational stability and functional motions of the S-protein, which are relevant for vaccine and antiviral drug developments.					
33484712	3	47	from	understanding	501:513	arg1	resolutions					552:562	atomic resolutions	545:562	atomic resolutions	545:562	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	4	48	theme	glycosylated	862:873	arg1	S-protein					875:883	a fully glycosylated S-protein	854:883	a fully glycosylated S-protein in solution	854:895	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	2	49	from	residues	347:354	arg1	surface					363:369	the surface	359:369	the surface	359:369	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	3	50	theme	down	740:743	arg1	forms					759:763	the inactive down and active up forms	727:763	the inactive down and active up forms	727:763	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	3	51	theme	S-protein	522:530	arg1	structure					532:540	the S-protein structure	518:540	the S-protein structure at atomic resolutions	518:562	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	1	52	theme	urgent	185:190	arg1	developments					192:203	urgent developments	185:203	urgent developments of vaccines and antiviral drugs	185:235	The ongoing COVID-19 pandemic caused by the new coronavirus, SARS-CoV-2, calls for urgent developments of vaccines and antiviral drugs.					
33484712	4	53	dep	dynamics	804:811	arg1	simulations					813:823	simulations	813:823	all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them	785:959	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	7	54	theme	receptor	1517:1524	arg1	domains					1534:1540	the three receptor binding domains	1507:1540	the three receptor binding domains	1507:1540	Electrostatic interactions, as well as hydrogen bonds between the three receptor binding domains, work as driving forces to initiate the conformational transitions toward the active form.					
33484712	2	55	theme	polypeptide	310:320	arg1	chains					322:327	trimeric polypeptide chains	301:327	trimeric polypeptide chains with glycosylated residues on the surface	301:369	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	8	56	theme	drug	1796:1799	arg1	developments					1801:1812	antiviral drug developments	1786:1812	antiviral drug developments	1786:1812	This study sheds light on the mechanisms underlying conformational stability and functional motions of the S-protein, which are relevant for vaccine and antiviral drug developments.					
33484712	4	57	gly	glycosylated	862:873	arg1	S-protein					875:883	a fully glycosylated S-protein	854:883	a fully glycosylated S-protein in solution	854:895	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	4	58	theme	forms	845:849	arg1	simulations					813:823	simulations	813:823	all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them	785:959	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	7	59	theme	hydrogen	1484:1491	arg1	bonds					1493:1497	hydrogen bonds	1484:1497	hydrogen bonds between the three receptor binding domains	1484:1540	Electrostatic interactions, as well as hydrogen bonds between the three receptor binding domains, work as driving forces to initiate the conformational transitions toward the active form.					
33484712	3	60	theme	active	749:754	arg1	forms					759:763	the inactive down and active up forms	727:763	the inactive down and active up forms	727:763	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	5	61	theme	interaction	1104:1114	arg1	analysis					1116:1123	The residue-level interaction analysis	1086:1123	The residue-level interaction analysis of the simulation trajectories	1086:1154	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	3	62	theme	structural	588:597	arg1	studies					599:605	these structural studies	582:605	these structural studies	582:605	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	4	63	theme	molecular	917:925	arg1	simulations					936:946	targeted molecular dynamics simulations	908:946	all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them	785:959	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	7	64	theme	Electrostatic	1445:1457	arg1	interactions					1459:1470	Electrostatic interactions	1445:1470	Electrostatic interactions	1445:1470	Electrostatic interactions, as well as hydrogen bonds between the three receptor binding domains, work as driving forces to initiate the conformational transitions toward the active form.					
33484712	3	65	from	resolutions	552:562	arg1	understanding					501:513	advanced our understanding	488:513	advanced our understanding of the S-protein structure at atomic resolutions	488:562	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	3	65	from	resolutions	552:562	arg1	structure					532:540	the S-protein structure	518:540	the S-protein structure at atomic resolutions	518:562	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	0	66	theme	SARS-CoV-2	80:89	arg1	S-protein					91:99	SARS-CoV-2 S-protein	80:99	SARS-CoV-2 S-protein	80:99	Elucidation of interactions regulating conformational stability and dynamics of SARS-CoV-2 S-protein.					
33484712	2	67	theme	glycosylated	334:345	arg1	residues					347:354	glycosylated residues	334:354	glycosylated residues on the surface	334:369	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	6	68	gly	glycosylated	1354:1365	arg1	residues					1367:1374	glycosylated residues	1354:1374	glycosylated residues	1354:1374	During the conformational transitions between them, interdomain interactions mediated by glycosylated residues are switched to play key roles on the stabilization of another form.					
33484712	5	69	theme	amino	1173:1177	arg1	determinants					1210:1221	determinants	1210:1221	determinants on conformational stability of each form	1210:1262	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	5	69	theme	amino	1173:1177	arg1	N-glycans					1197:1205	N-glycans	1197:1205	N-glycans	1197:1205	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	5	69	theme	amino	1173:1177	arg1	residues					1184:1191	distinct amino acid residues	1164:1191	distinct amino acid residues	1164:1191	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	4	70	theme	interdomain	978:988	arg1	interactions					990:1001	key interdomain interactions	974:1001	key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions	974:1083	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	6	71	theme	form	1439:1442	arg1	stabilization					1414:1426	the stabilization	1410:1426	the stabilization of another form	1410:1442	During the conformational transitions between them, interdomain interactions mediated by glycosylated residues are switched to play key roles on the stabilization of another form.					
33484712	3	72	contain	have	475:478	arg1	studies					451:457	Extensive structural and functional studies	415:457	Extensive structural and functional studies on this protein	415:473	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	3	72	contain	have	475:478	arg2	understanding					501:513	advanced our understanding	488:513	advanced our understanding of the S-protein structure at atomic resolutions	488:562	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	5	73	theme	simulation	1132:1141	arg1	trajectories					1143:1154	the simulation trajectories	1128:1154	the simulation trajectories	1128:1154	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	4	74	dep	both	828:831	arg1	down					833:836	down	833:836	down	833:836	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	3	75	from	effect	620:625	arg1	stability					686:694	conformational stability	671:694	conformational stability	671:694	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	3	75	from	effect	620:625	arg1	motions					711:717	functional motions	700:717	functional motions	700:717	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	2	76	theme	virus	385:389	arg1	entry					391:395	the virus entry	381:395	the virus entry into a host cell	381:412	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	4	77	from	forms	845:849	arg1	solution					888:895	solution	888:895	solution	888:895	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
33484712	3	78	theme	Extensive	415:423	arg1	studies					451:457	Extensive structural and functional studies	415:457	Extensive structural and functional studies on this protein	415:473	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	5	79	theme	conformational	1226:1239	arg1	stability					1241:1249	conformational stability	1226:1249	conformational stability of each form	1226:1262	The residue-level interaction analysis of the simulation trajectories detects distinct amino acid residues and N-glycans as determinants on conformational stability of each form.					
33484712	1	80	theme	COVID-19	114:121	arg1	pandemic					123:130	The ongoing COVID-19 pandemic	102:130	The ongoing COVID-19 pandemic	102:130	The ongoing COVID-19 pandemic caused by the new coronavirus, SARS-CoV-2, calls for urgent developments of vaccines and antiviral drugs.					
33484712	2	81	theme	spike	242:246	arg1	S-protein					271:279	S-protein	271:279	S-protein	271:279	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	2	81	theme	spike	242:246	arg1	protein					248:254	The spike protein	238:254	The spike protein of SARS-CoV-2 (S-protein)	238:280	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	2	82	theme	host	404:407	arg1	cell					409:412	a host cell	402:412	a host cell	402:412	The spike protein of SARS-CoV-2 (S-protein), which consists of trimeric polypeptide chains with glycosylated residues on the surface, triggers the virus entry into a host cell.					
33484712	7	83	theme	active	1620:1625	arg1	form					1627:1630	the active form	1616:1630	the active form	1616:1630	Electrostatic interactions, as well as hydrogen bonds between the three receptor binding domains, work as driving forces to initiate the conformational transitions toward the active form.					
33484712	3	84	theme	glycans	630:636	arg1	effect					620:625	the effect	616:625	the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms	616:763	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	3	85	theme	advanced	488:495	arg1	understanding					501:513	advanced our understanding	488:513	advanced our understanding of the S-protein structure at atomic resolutions	488:562	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	8	86	theme	S-protein	1740:1748	arg1	stability					1700:1708	conformational stability	1685:1708	conformational stability	1685:1708	This study sheds light on the mechanisms underlying conformational stability and functional motions of the S-protein, which are relevant for vaccine and antiviral drug developments.					
33484712	8	86	theme	S-protein	1740:1748	arg1	motions					1725:1731	functional motions	1714:1731	functional motions	1714:1731	This study sheds light on the mechanisms underlying conformational stability and functional motions of the S-protein, which are relevant for vaccine and antiviral drug developments.					
33484712	7	87	theme	conformational	1582:1595	arg1	transitions					1597:1607	the conformational transitions	1578:1607	the conformational transitions toward the active form	1578:1630	Electrostatic interactions, as well as hydrogen bonds between the three receptor binding domains, work as driving forces to initiate the conformational transitions toward the active form.					
33484712	3	88	theme	functional	700:709	arg1	motions					711:717	functional motions	700:717	functional motions	700:717	Extensive structural and functional studies on this protein have rapidly advanced our understanding of the S-protein structure at atomic resolutions, although most of these structural studies overlook the effect of glycans attached to the S-protein on the conformational stability and functional motions between the inactive down and active up forms.					
33484712	4	89	theme	molecular	794:802	arg1	dynamics					804:811	all-atom molecular dynamics	785:811	all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them	785:959	Here, we performed all-atom molecular dynamics simulations of both down and up forms of a fully glycosylated S-protein in solution as well as targeted molecular dynamics simulations between them to elucidate key interdomain interactions for stabilizing each form and inducing the large-scale conformational transitions.					
32306337	5	0	theme	CRT	835:837	arg1	isolation					805:813	isolation	805:813	isolation	805:813	This chapter describes the isolation and purification of CRT produced in a bacterial expression system.					
32306337	5	0	theme	CRT	835:837	arg1	purification					819:830	purification	819:830	purification	819:830	This chapter describes the isolation and purification of CRT produced in a bacterial expression system.					
32306337	4	1	theme	glycan-dependent	729:744	arg1	manners					769:775	glycan-dependent and glycan-independent manners	729:775	glycan-dependent and glycan-independent manners	729:775	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	4	2	theme	glycan-independent	750:767	arg1	manners					769:775	glycan-dependent and glycan-independent manners	729:775	glycan-dependent and glycan-independent manners	729:775	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	1	3	theme	molecular	143:151	arg1	Calnexin					77:84	Calnexin	77:84	Calnexin (CNX)	77:90	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	1	3	theme	molecular	143:151	arg1	calreticulin					96:107	calreticulin	96:107	calreticulin (CRT)	96:113	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	1	3	theme	molecular	143:151	arg1	chaperones					153:162	ER-resident lectin-like molecular chaperones	119:162	ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins	119:233	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	4	4	theme	lectin-like	535:545	arg1	interactions					547:558	the lectin-like interactions	531:558	the lectin-like interactions	531:558	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	1	5	gly	glycoproteins	221:233	arg1	glycoproteins					221:233	secretory or membrane glycoproteins	199:233	secretory or membrane glycoproteins	199:233	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	6	6	theme	target	1056:1061	arg1	α-mannosidase					1037:1049	Jack bean α-mannosidase	1027:1049	Jack bean α-mannosidase	1027:1049	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	6	6	theme	target	1056:1061	arg1	substrate					1063:1071	a target substrate	1054:1071	a target substrate	1054:1071	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	4	7	dep	interactions	547:558	arg1	addition					519:526	addition	519:526	addition	519:526	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	4	8	theme	substrates	623:632	arg1	aggregation					591:601	the aggregation	587:601	the aggregation of non-glycosylated substrates	587:632	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	6	9	theme	CRT	965:967	arg1	functions					952:960	the molecular chaperone functions	928:960	the molecular chaperone functions of CRT	928:967	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	2	10	theme	specific	285:292	arg1	binding					294:300	specific binding	285:300	specific binding to the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins	285:393	They can exert molecular chaperone functions via specific binding to the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins.					
32306337	2	11	theme	N-glycoproteins	379:393	arg1	oligosaccharides					359:374	Glc1Man9GlcNAc2 oligosaccharides	343:374	Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins	343:393	They can exert molecular chaperone functions via specific binding to the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins.					
32306337	4	12	from	function	717:724	arg1	manners					769:775	glycan-dependent and glycan-independent manners	729:775	glycan-dependent and glycan-independent manners	729:775	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	4	13	theme	non-glycosylated	606:621	arg1	substrates					623:632	non-glycosylated substrates	606:632	non-glycosylated substrates	606:632	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	3	14	theme	same	462:465	arg1	structure					487:495	the same jelly roll tertiary structure	458:495	the same jelly roll tertiary structure as legume lectins	458:513	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	5	15	theme	expression	863:872	arg1	system					874:879	a bacterial expression system	851:879	a bacterial expression system	851:879	This chapter describes the isolation and purification of CRT produced in a bacterial expression system.					
32306337	7	16	theme	lectin	1179:1184	arg1	functions					1186:1194	lectin functions	1179:1194	lectin functions	1179:1194	These assays are valuable in assessing quality control events related to the CNX/CRT chaperone cycle and lectin functions.					
32306337	3	17	theme	jelly	467:471	arg1	structure					487:495	the same jelly roll tertiary structure	458:495	the same jelly roll tertiary structure as legume lectins	458:513	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	6	18	theme	chaperone	942:950	arg1	functions					952:960	the molecular chaperone functions	928:960	the molecular chaperone functions of CRT	928:967	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	7	19	theme	chaperone	1159:1167	arg1	cycle					1169:1173	the CNX/CRT chaperone cycle	1147:1173	the CNX/CRT chaperone cycle	1147:1173	These assays are valuable in assessing quality control events related to the CNX/CRT chaperone cycle and lectin functions.					
32306337	2	20	theme	processing	315:324	arg1	intermediates					326:338	the early processing intermediates	305:338	the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins	305:393	They can exert molecular chaperone functions via specific binding to the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins.					
32306337	3	21	theme	similar	413:419	arg1	domains					440:446	similar N-terminal luminal domains	413:446	similar N-terminal luminal domains	413:446	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	6	22	dep	in	900:901	arg1	vitro					903:907	vitro	903:907	vitro	903:907	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	4	23	gly	non-glycosylated	606:621	arg1	substrates					623:632	non-glycosylated substrates	606:632	non-glycosylated substrates	606:632	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	2	24	theme	early	309:313	arg1	intermediates					326:338	the early processing intermediates	305:338	the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins	305:393	They can exert molecular chaperone functions via specific binding to the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins.					
32306337	1	25	theme	quality	180:186	arg1	control					188:194	the quality control	176:194	the quality control of secretory or membrane glycoproteins	176:233	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	3	26	contain	have	408:411	arg2	domains					440:446	similar N-terminal luminal domains	413:446	similar N-terminal luminal domains	413:446	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	3	26	contain	have	408:411	arg1	CRT					404:406	CRT	404:406	CRT	404:406	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	3	26	contain	have	408:411	arg1	CNX					396:398	CNX	396:398	CNX	396:398	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	7	27	theme	CNX/CRT	1151:1157	arg1	cycle					1169:1173	the CNX/CRT chaperone cycle	1147:1173	the CNX/CRT chaperone cycle	1147:1173	These assays are valuable in assessing quality control events related to the CNX/CRT chaperone cycle and lectin functions.					
32306337	6	28	theme	molecular	932:940	arg1	functions					952:960	the molecular chaperone functions	928:960	the molecular chaperone functions of CRT	928:967	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	4	29	theme	peptide	671:677	arg1	parts					679:683	hydrophobic peptide parts	659:683	hydrophobic peptide parts	659:683	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	0	30	theme	N-Glycoprotein	44:57	arg1	Folding					59:65	N-Glycoprotein Folding	44:65	N-Glycoprotein Folding	44:65	Calnexin/Calreticulin and Assays Related to N-Glycoprotein Folding In Vitro.					
32306337	6	31	theme	bean	1032:1035	arg1	α-mannosidase					1037:1049	Jack bean α-mannosidase	1027:1049	Jack bean α-mannosidase	1027:1049	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	6	31	theme	bean	1032:1035	arg1	substrate					1063:1071	a target substrate	1054:1071	a target substrate	1054:1071	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	7	32	theme	related	1136:1142	arg1	events					1129:1134	quality control events	1113:1134	quality control events related to the CNX/CRT chaperone cycle and lectin functions	1113:1194	These assays are valuable in assessing quality control events related to the CNX/CRT chaperone cycle and lectin functions.					
32306337	3	33	theme	roll	473:476	arg1	structure					487:495	the same jelly roll tertiary structure	458:495	the same jelly roll tertiary structure as legume lectins	458:513	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	4	34	theme	chaperone	707:715	arg1	function					717:724	a general chaperone function	697:724	a general chaperone function in glycan-dependent and glycan-independent manners	697:775	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	1	35	theme	secretory	199:207	arg1	glycoproteins					221:233	secretory or membrane glycoproteins	199:233	secretory or membrane glycoproteins	199:233	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	4	36	theme	general	699:705	arg1	function					717:724	a general chaperone function	697:724	a general chaperone function in glycan-dependent and glycan-independent manners	697:775	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	6	37	theme	Jack	1027:1030	arg1	α-mannosidase					1037:1049	Jack bean α-mannosidase	1027:1049	Jack bean α-mannosidase	1027:1049	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	6	37	theme	Jack	1027:1030	arg1	substrate					1063:1071	a target substrate	1054:1071	a target substrate	1054:1071	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	2	38	gly	N-glycoproteins	379:393	arg1	N-glycoproteins					379:393	N-glycoproteins	379:393	N-glycoproteins	379:393	They can exert molecular chaperone functions via specific binding to the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins.					
32306337	1	39	theme	or	209:210	arg1	glycoproteins					221:233	secretory or membrane glycoproteins	199:233	secretory or membrane glycoproteins	199:233	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	3	40	theme	N-terminal	421:430	arg1	domains					440:446	similar N-terminal luminal domains	413:446	similar N-terminal luminal domains	413:446	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	7	41	theme	control	1121:1127	arg1	events					1129:1134	quality control events	1113:1134	quality control events related to the CNX/CRT chaperone cycle and lectin functions	1113:1194	These assays are valuable in assessing quality control events related to the CNX/CRT chaperone cycle and lectin functions.					
32306337	4	42	with	interaction	642:652	arg1	parts					679:683	hydrophobic peptide parts	659:683	hydrophobic peptide parts	659:683	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	2	43	theme	oligosaccharides	359:374	arg1	intermediates					326:338	the early processing intermediates	305:338	the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins	305:393	They can exert molecular chaperone functions via specific binding to the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins.					
32306337	3	44	theme	luminal	432:438	arg1	domains					440:446	similar N-terminal luminal domains	413:446	similar N-terminal luminal domains	413:446	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	2	45	theme	Glc1Man9GlcNAc2	343:357	arg1	oligosaccharides					359:374	Glc1Man9GlcNAc2 oligosaccharides	343:374	Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins	343:393	They can exert molecular chaperone functions via specific binding to the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins.					
32306337	6	46	theme	monoglucosylated	994:1009	arg1	N-glycans					1011:1019	monoglucosylated N-glycans	994:1019	monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate	994:1071	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	2	47	theme	chaperone	261:269	arg1	functions					271:279	molecular chaperone functions	251:279	molecular chaperone functions	251:279	They can exert molecular chaperone functions via specific binding to the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins.					
32306337	3	48	theme	legume	500:505	arg1	lectins					507:513	legume lectins	500:513	legume lectins	500:513	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	1	49	theme	membrane	212:219	arg1	glycoproteins					221:233	secretory or membrane glycoproteins	199:233	secretory or membrane glycoproteins	199:233	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	7	50	theme	quality	1113:1119	arg1	events					1129:1134	quality control events	1113:1134	quality control events related to the CNX/CRT chaperone cycle and lectin functions	1113:1194	These assays are valuable in assessing quality control events related to the CNX/CRT chaperone cycle and lectin functions.					
32306337	6	51	theme	in	900:901	arg1	assays					909:914	in vitro assays	900:914	in vitro assays	900:914	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	2	52	theme	molecular	251:259	arg1	functions					271:279	molecular chaperone functions	251:279	molecular chaperone functions	251:279	They can exert molecular chaperone functions via specific binding to the early processing intermediates of Glc1Man9GlcNAc2 oligosaccharides of N-glycoproteins.					
32306337	6	53	with	interaction	977:987	arg1	N-glycans					1011:1019	monoglucosylated N-glycans	994:1019	monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate	994:1071	We also introduce in vitro assays to estimate the molecular chaperone functions of CRT via the interaction with monoglucosylated N-glycans using Jack bean α-mannosidase as a target substrate.					
32306337	1	54	theme	glycoproteins	221:233	arg1	control					188:194	the quality control	176:194	the quality control of secretory or membrane glycoproteins	176:233	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	3	55	theme	tertiary	478:485	arg1	structure					487:495	the same jelly roll tertiary structure	458:495	the same jelly roll tertiary structure as legume lectins	458:513	CNX and CRT have similar N-terminal luminal domains and share the same jelly roll tertiary structure as legume lectins.					
32306337	1	56	theme	ER-resident	119:129	arg1	Calnexin					77:84	Calnexin	77:84	Calnexin (CNX)	77:90	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	1	56	theme	ER-resident	119:129	arg1	calreticulin					96:107	calreticulin	96:107	calreticulin (CRT)	96:113	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	1	56	theme	ER-resident	119:129	arg1	chaperones					153:162	ER-resident lectin-like molecular chaperones	119:162	ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins	119:233	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	4	57	theme	hydrophobic	659:669	arg1	parts					679:683	hydrophobic peptide parts	659:683	hydrophobic peptide parts	659:683	In addition to the lectin-like interactions, CNX and CRT also suppress the aggregation of non-glycosylated substrates through interaction with hydrophobic peptide parts, suggesting a general chaperone function in glycan-dependent and glycan-independent manners.					
32306337	5	58	theme	bacterial	853:861	arg1	system					874:879	a bacterial expression system	851:879	a bacterial expression system	851:879	This chapter describes the isolation and purification of CRT produced in a bacterial expression system.					
32306337	1	59	theme	lectin-like	131:141	arg1	Calnexin					77:84	Calnexin	77:84	Calnexin (CNX)	77:90	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	1	59	theme	lectin-like	131:141	arg1	calreticulin					96:107	calreticulin	96:107	calreticulin (CRT)	96:113	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
32306337	1	59	theme	lectin-like	131:141	arg1	chaperones					153:162	ER-resident lectin-like molecular chaperones	119:162	ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins	119:233	Calnexin (CNX) and calreticulin (CRT) are ER-resident lectin-like molecular chaperones involved in the quality control of secretory or membrane glycoproteins.					
33901620	10	0	theme	enzymes	1852:1858	arg1	activation					1811:1820	an activation	1808:1820	an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor	1808:1960	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	5	1	theme	Neu5Gc	1062:1067	arg1	content					1069:1075	the Neu5Gc content	1058:1075	the Neu5Gc content of sialic acid	1058:1090	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	5	1	theme	Neu5Gc	1062:1067	arg1	%					1110:1110	<3%	1108:1110	<3%	1108:1110	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	5	1	theme	Neu5Gc	1062:1067	arg1	negligible					1096:1105	negligible	1096:1105	negligible	1096:1105	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	10	2	theme	galactosyl	1829:1838	arg1	transferase					1840:1850	galactosyl transferase	1829:1850	the galactosyl transferase enzymes	1825:1858	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	11	3	theme	combinations	2168:2179	arg1	presence					2156:2163	the presence	2152:2163	the presence of combinations of the MGU supplements	2152:2202	However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements.					
33901620	3	4	theme	used	517:520	arg1	supplements					528:538	a commonly used media supplements	506:538	a commonly used media supplements of manganese, galactose and uridine (MGU)	506:580	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	8	5	theme	components	1487:1496	arg1	uridine					1499:1505	uridine	1499:1505	uridine	1499:1505	We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.					
33901620	8	5	theme	components	1487:1496	arg1	one					1476:1478	one	1476:1478	one	1476:1478	We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.					
33901620	8	5	theme	components	1487:1496	arg1	components					1487:1496	the components	1483:1496	the components (uridine)	1483:1506	We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.					
33901620	10	6	theme	increased	1731:1739	arg1	level					1741:1745	The increased level	1727:1745	The increased level of galactosylation observed with MG or MGU	1727:1788	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	4	7	theme	IgG1	782:785	arg1	antibody					787:794	the humanized IgG1 antibody	768:794	the humanized IgG1 antibody (Hu1D10) produced from NS0 cells	768:827	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	4	7	theme	IgG1	782:785	arg1	Hu1D10					797:802	Hu1D10	797:802	Hu1D10	797:802	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	11	8	theme	Galα1-3	2106:2112	arg1	Gal					2114:2116	Galα1-3 Gal	2106:2116	Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements	2106:2202	However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements.					
33901620	8	9	from	increase	1389:1396	arg1	sialylation					1401:1411	sialylation	1401:1411	sialylation of the EG2-hFc Mab	1401:1430	We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.					
33901620	12	10	from	increase	2334:2341	arg1	cells					2381:2385	NS0 cells	2377:2385	NS0 cells	2377:2385	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	12	10	from	increase	2334:2341	arg1	sialylation					2350:2360	the sialylation	2346:2360	the sialylation of Hu1D10 from NS0 cells	2346:2385	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	10	11	theme	enhanced	1868:1875	arg1	levels					1891:1896	enhanced intracellular levels	1868:1896	enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor	1868:1960	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	3	12	theme	manganese	543:551	arg1	supplements					528:538	a commonly used media supplements	506:538	a commonly used media supplements of manganese, galactose and uridine (MGU)	506:580	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	0	13	theme	monoclonal	102:111	arg1	antibodies					113:122	monoclonal antibodies	102:122	monoclonal antibodies	102:122	Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies.					
33901620	6	14	from	GI:0.78	1261:1267	arg1	Hu1D10					1272:1277	Hu1D10	1272:1277	Hu1D10	1272:1277	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	3	15	theme	supplements	528:538	arg1	effects					495:501	the effects	491:501	the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes	491:727	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	4	16	theme	mono-	851:855	arg1	structures					875:884	mono- and di-sialylated structures	851:884	mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc	851:934	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	4	16	theme	mono-	851:855	arg1	SI:0.09					887:893	SI:0.09	887:893	SI:0.09	887:893	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	5	17	dep	negligible	1096:1105	arg1	%					1110:1110	<3%	1108:1110	<3%	1108:1110	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	5	17	dep	negligible	1096:1105	arg1	content					1069:1075	the Neu5Gc content	1058:1075	the Neu5Gc content of sialic acid	1058:1090	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	5	17	dep	negligible	1096:1105	arg1	negligible					1096:1105	negligible	1096:1105	negligible	1096:1105	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	10	18	theme	UDP-Gal	1901:1907	arg1	levels					1891:1896	enhanced intracellular levels	1868:1896	enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor	1868:1960	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	10	19	theme	galactosylation	1750:1764	arg1	level					1741:1745	The increased level	1727:1745	The increased level of galactosylation observed with MG or MGU	1727:1788	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	3	20	theme	uridine	568:574	arg1	supplements					528:538	a commonly used media supplements	506:538	a commonly used media supplements of manganese, galactose and uridine (MGU)	506:580	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	1	21	theme	therapeutic	209:219	arg1	antibodies					232:241	therapeutic monoclonal antibodies	209:241	therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0	209:313	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	6	22	dep	resulted	1183:1190	arg1	GI:0.78					1261:1267	GI:0.78	1261:1267	GI:0.78	1261:1267	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	6	22	dep	resulted	1183:1190	arg1	0.81					1283:1286	0.81	1283:1286	0.81	1283:1286	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	9	23	theme	Neu5Gc	1719:1724	arg1	formation					1687:1695	the formation	1683:1695	the formation of Neu5Ac rather than Neu5Gc	1683:1724	On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc.					
33901620	3	24	theme	glycan	713:718	arg1	epitopes					720:727	these non-human glycan epitopes	697:727	these non-human glycan epitopes	697:727	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	3	25	theme	galactose	554:562	arg1	supplements					528:538	a commonly used media supplements	506:538	a commonly used media supplements of manganese, galactose and uridine (MGU)	506:580	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	12	26	from	cells	2381:2385	arg1	increase					2334:2341	a significant increase	2320:2341	a significant increase in the sialylation of Hu1D10 from NS0 cells	2320:2385	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	12	26	from	cells	2381:2385	arg1	sialylation					2350:2360	the sialylation	2346:2360	the sialylation of Hu1D10 from NS0 cells	2346:2385	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	13	27	from	decrease	2424:2431	arg1	proportion					2440:2449	the proportion	2436:2449	the proportion of Neu5Gc in the measured sialic acid from the Mab	2436:2500	This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.					
33901620	6	28	theme	MGU	1134:1136	arg1	supplements					1138:1148	the MGU supplements	1130:1148	the MGU supplements added to the production cultures	1130:1181	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	5	29	theme	human-llama	950:960	arg1	EG2-hFc					967:973	EG2-hFc	967:973	EG2-hFc	967:973	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	5	29	theme	human-llama	950:960	arg1	Mab					962:964	The chimeric human-llama Mab	937:964	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells	937:998	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	12	30	with	Supplementation	2205:2219	arg1	FBS					2255:2257	FBS	2255:2257	FBS	2255:2257	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	12	30	with	Supplementation	2205:2219	arg1	serum					2248:2252	fetal bovine serum	2235:2252	fetal bovine serum (FBS)	2235:2258	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	11	31	theme	supplements	2192:2202	arg1	combinations					2168:2179	combinations	2168:2179	combinations of the MGU supplements	2168:2202	However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements.					
33901620	10	32	theme	enzymic	1944:1950	arg1	co-factor					1952:1960	an enzymic co-factor	1941:1960	an enzymic co-factor	1941:1960	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	0	33	theme	glycan	58:63	arg1	profiles					65:72	glycan profiles	58:72	glycan profiles	58:72	Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies.					
33901620	5	34	theme	low	1018:1020	arg1	level					1022:1026	an equally low level	1007:1026	an equally low level of sialylation (SI: 0.12)	1007:1052	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	1	35	theme	profiles	154:161	arg1	essential					166:174	essential	166:174	essential	166:174	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	1	35	theme	profiles	154:161	arg1	control					129:135	The control	125:135	The control of glycosylation profiles	125:161	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	9	36	theme	other	1546:1550	arg1	hand					1552:1555	the other hand	1542:1555	the other hand	1542:1555	On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc.					
33901620	6	37	theme	substantial	1197:1207	arg1	increase					1209:1216	a substantial increase	1195:1216	a substantial increase in the galactosylation of both Mabs	1195:1252	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	9	38	gly	sialylation	1578:1588	arg1	Hu1D10					1593:1598	Hu1D10	1593:1598	Hu1D10	1593:1598	On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc.					
33901620	0	39	theme	cultures	38:45	arg1	feeding					10:16	Strategic feeding	0:16	Strategic feeding of NS0 and CHO cell cultures to	0:48	Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies.					
33901620	5	40	dep	SI	1044:1045	arg1	0.12					1048:1051	0.12	1048:1051	SI: 0.12	1044:1051	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	6	41	from	increase	1209:1216	arg1	galactosylation					1225:1239	the galactosylation	1221:1239	the galactosylation of both Mabs	1221:1252	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	2	42	theme	Galα1-3	436:442	arg1	Gal					444:446	Galα1-3 Gal	436:446	Galα1-3 Gal	436:446	Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic.					
33901620	12	43	theme	Neu5Ac	2295:2300	arg1	availability					2274:2285	the availability	2270:2285	the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells	2270:2385	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	2	44	theme	non-human	370:378	arg1	epitopes					380:387	non-human epitopes	370:387	non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic	370:470	Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic.					
33901620	2	44	theme	non-human	370:378	arg1	acid					418:421	N-glycolylneuraminic acid	397:421	N-glycolylneuraminic acid (Neu5Gc)	397:430	Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic.					
33901620	2	44	theme	non-human	370:378	arg1	Gal					444:446	Galα1-3 Gal	436:446	Galα1-3 Gal	436:446	Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic.					
33901620	0	45	theme	Strategic	0:8	arg1	feeding					10:16	Strategic feeding	0:16	Strategic feeding of NS0 and CHO cell cultures to	0:48	Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies.					
33901620	4	46	gly	di-sialylated	861:873	arg1	structures					875:884	mono- and di-sialylated structures	851:884	mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc	851:934	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	4	46	gly	di-sialylated	861:873	arg1	SI:0.09					887:893	SI:0.09	887:893	SI:0.09	887:893	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	1	47	theme	cell	282:285	arg1	lines					287:291	cell lines	282:291	cell lines	282:291	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	3	48	theme	CHO	605:607	arg1	cells					617:621	CHO and NS0 cells	605:621	cells	617:621	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	4	49	theme	supplement	756:765	arg1	absence					737:743	the absence	733:743	the absence of the MGU supplement	733:765	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	2	50	theme	N-glycolylneuraminic	397:416	arg1	Neu5Gc					424:429	Neu5Gc	424:429	Neu5Gc	424:429	Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic.					
33901620	2	50	theme	N-glycolylneuraminic	397:416	arg1	acid					418:421	N-glycolylneuraminic acid	397:421	N-glycolylneuraminic acid (Neu5Gc)	397:430	Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic.					
33901620	4	51	theme	sialic	913:918	arg1	acid					920:923	sialic acid	913:923	sialic acid	913:923	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	8	52	theme	slight	1382:1387	arg1	increase					1389:1396	a slight increase	1380:1396	a slight increase in sialylation of the EG2-hFc Mab	1380:1430	We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.					
33901620	2	53	theme	concern	330:336	arg1	potential					345:353	the potential	341:353	the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic	341:470	Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic.					
33901620	0	54	theme	cell	33:36	arg1	cultures					38:45	NS0 and CHO cell cultures	21:45	cultures	38:45	Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies.					
33901620	13	55	from	Mab	2498:2500	arg1	acid					2484:2487	the measured sialic acid	2464:2487	the measured sialic acid from the Mab	2464:2500	This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.					
33901620	13	55	from	Mab	2498:2500	arg1	proportion					2440:2449	the proportion	2436:2449	the proportion of Neu5Gc in the measured sialic acid from the Mab	2436:2500	This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.					
33901620	1	56	theme	monoclonal	221:230	arg1	antibodies					232:241	therapeutic monoclonal antibodies	209:241	therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0	209:313	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	12	57	theme	free	2290:2293	arg1	Neu5Ac					2295:2300	free Neu5Ac	2290:2300	free Neu5Ac	2290:2300	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	11	58	theme	beta	2017:2020	arg1	linkage					2030:2036	the desirable beta 1-4 Gal linkage	2003:2036	the desirable beta 1-4 Gal linkage to GlcNAc	2003:2046	However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements.					
33901620	3	59	theme	NS0	613:615	arg1	cells					617:621	CHO and NS0 cells	605:621	cells	617:621	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	13	60	theme	sialic	2477:2482	arg1	acid					2484:2487	the measured sialic acid	2464:2487	the measured sialic acid from the Mab	2464:2500	This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.					
33901620	7	61	from	effects	1314:1320	arg1	sialylation					1325:1335	sialylation	1325:1335	sialylation	1325:1335	However, the effects on sialylation differed between the two Mabs.					
33901620	3	62	from	cells	617:621	arg1	production					589:598	Mab production	585:598	Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes	585:727	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	10	63	theme	transferase	1840:1850	arg1	enzymes					1852:1858	the galactosyl transferase enzymes	1825:1858	the galactosyl transferase enzymes	1825:1858	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	8	64	theme	Mab	1428:1430	arg1	sialylation					1401:1411	sialylation	1401:1411	sialylation of the EG2-hFc Mab	1401:1430	We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.					
33901620	13	65	theme	Neu5Gc	2454:2459	arg1	proportion					2440:2449	the proportion	2436:2449	the proportion of Neu5Gc in the measured sialic acid from the Mab	2436:2500	This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.					
33901620	11	66	theme	Gal	2026:2028	arg1	linkage					2030:2036	the desirable beta 1-4 Gal linkage	2003:2036	the desirable beta 1-4 Gal linkage to GlcNAc	2003:2046	However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements.					
33901620	12	67	theme	bovine	2241:2246	arg1	FBS					2255:2257	FBS	2255:2257	FBS	2255:2257	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	12	67	theme	bovine	2241:2246	arg1	serum					2248:2252	fetal bovine serum	2235:2252	fetal bovine serum (FBS)	2235:2258	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	9	68	dep	substantially	1600:1612	arg1	SI:0.72					1615:1621	SI:0.72	1615:1621	SI:0.72	1615:1621	On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc.					
33901620	3	69	from	effects	495:501	arg1	production					589:598	Mab production	585:598	Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes	585:727	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	13	70	theme	significant	2412:2422	arg1	decrease					2424:2431	a significant decrease	2410:2431	a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab	2410:2500	This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.					
33901620	4	71	theme	humanized	772:780	arg1	antibody					787:794	the humanized IgG1 antibody	768:794	the humanized IgG1 antibody (Hu1D10) produced from NS0 cells	768:827	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	4	71	theme	humanized	772:780	arg1	Hu1D10					797:802	Hu1D10	797:802	Hu1D10	797:802	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	13	72	from	proportion	2440:2449	arg1	acid					2484:2487	the measured sialic acid	2464:2487	the measured sialic acid from the Mab	2464:2500	This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.					
33901620	13	72	from	proportion	2440:2449	arg1	Mab					2498:2500	the Mab	2494:2500	the Mab	2494:2500	This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.					
33901620	10	73	theme	intracellular	1877:1889	arg1	levels					1891:1896	enhanced intracellular levels	1868:1896	enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor	1868:1960	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	10	74	theme	availability	1917:1928	arg1	levels					1891:1896	enhanced intracellular levels	1868:1896	enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor	1868:1960	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	3	75	theme	non-human	703:711	arg1	epitopes					720:727	these non-human glycan epitopes	697:727	these non-human glycan epitopes	697:727	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	0	76	theme	antibodies	113:122	arg1	profiles					65:72	glycan profiles	58:72	glycan profiles	58:72	Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies.					
33901620	0	76	theme	antibodies	113:122	arg1	epitopes					90:97	immunogenic epitopes	78:97	immunogenic epitopes	78:97	Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies.					
33901620	3	77	theme	media	522:526	arg1	supplements					528:538	a commonly used media supplements	506:538	a commonly used media supplements of manganese, galactose and uridine (MGU)	506:580	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	1	78	theme	consistent	183:192	arg1	manufacture					194:204	the consistent manufacture	179:204	the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0	179:313	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	4	79	theme	low	838:840	arg1	level					842:846	a low level	836:846	a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc	836:934	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	9	80	theme	Neu5Ac	1700:1705	arg1	formation					1687:1695	the formation	1683:1695	the formation of Neu5Ac rather than Neu5Gc	1683:1724	On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc.					
33901620	6	81	theme	production	1163:1172	arg1	cultures					1174:1181	the production cultures	1159:1181	the production cultures	1159:1181	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	12	82	theme	NS0	2377:2379	arg1	cells					2381:2385	NS0 cells	2377:2385	NS0 cells	2377:2385	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	4	83	theme	di-sialylated	861:873	arg1	structures					875:884	mono- and di-sialylated structures	851:884	mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc	851:934	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	4	83	theme	di-sialylated	861:873	arg1	SI:0.09					887:893	SI:0.09	887:893	SI:0.09	887:893	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	4	84	theme	NS0	819:821	arg1	cells					823:827	NS0 cells	819:827	NS0 cells	819:827	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	10	85	theme	Mn2+	1933:1936	arg1	UDP-Gal					1901:1907	UDP-Gal	1901:1907	UDP-Gal	1901:1907	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	10	85	theme	Mn2+	1933:1936	arg1	availability					1917:1928	the availability	1913:1928	the availability of Mn2+ as an enzymic co-factor	1913:1960	The increased level of galactosylation observed with MG or MGU was attributed to an activation of the galactosyl transferase enzymes through enhanced intracellular levels of UDP-Gal and the availability of Mn2+ as an enzymic co-factor.					
33901620	3	86	theme	epitopes	720:727	arg1	generation					683:692	the generation	679:692	galactosylation and sialylation as well as the generation of these non-human glycan epitopes	636:727	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	3	86	theme	epitopes	720:727	arg1	sialylation					656:666	sialylation	656:666	sialylation	656:666	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	3	86	theme	epitopes	720:727	arg1	galactosylation					636:650	galactosylation	636:650	galactosylation	636:650	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	6	87	theme	supplements	1138:1148	arg1	Combinations					1114:1125	Combinations	1114:1125	Combinations of the MGU supplements added to the production cultures	1114:1181	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	5	88	theme	chimeric	941:948	arg1	EG2-hFc					967:973	EG2-hFc	967:973	EG2-hFc	967:973	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	5	88	theme	chimeric	941:948	arg1	Mab					962:964	The chimeric human-llama Mab	937:964	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells	937:998	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	11	89	theme	MGU	2188:2190	arg1	supplements					2192:2202	the MGU supplements	2184:2202	the MGU supplements	2184:2202	However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements.					
33901620	12	90	theme	Hu1D10	2365:2370	arg1	sialylation					2350:2360	the sialylation	2346:2360	the sialylation of Hu1D10 from NS0 cells	2346:2385	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	5	91	theme	sialic	1080:1085	arg1	acid					1087:1090	sialic acid	1080:1090	sialic acid	1080:1090	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	12	92	gly	sialylation	2350:2360	arg1	Hu1D10					2365:2370	Hu1D10	2365:2370	Hu1D10	2365:2370	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	1	93	theme	glycosylation	140:152	arg1	profiles					154:161	glycosylation profiles	140:161	glycosylation profiles	140:161	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	12	94	theme	significant	2322:2332	arg1	increase					2334:2341	a significant increase	2320:2341	a significant increase in the sialylation of Hu1D10 from NS0 cells	2320:2385	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	0	95	theme	immunogenic	78:88	arg1	epitopes					90:97	immunogenic epitopes	78:97	immunogenic epitopes	78:97	Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies.					
33901620	11	96	theme	desirable	2007:2015	arg1	linkage					2030:2036	the desirable beta 1-4 Gal linkage	2003:2036	the desirable beta 1-4 Gal linkage to GlcNAc	2003:2046	However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements.					
33901620	11	97	theme	Gal	2114:2116	arg1	formation					2093:2101	the formation	2089:2101	the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements	2089:2202	However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements.					
33901620	12	98	theme	media	2224:2228	arg1	Supplementation					2205:2219	Supplementation	2205:2219	Supplementation of media with fetal bovine serum (FBS)	2205:2258	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	5	99	theme	CHO	990:992	arg1	cells					994:998	CHO cells	990:998	CHO cells	990:998	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	9	100	gly	increased	1568:1576	arg1	hand					1552:1555	the other hand	1542:1555	the other hand	1542:1555	On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc.					
33901620	4	101	theme	structures	875:884	arg1	level					842:846	a low level	836:846	a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc	836:934	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	4	101	theme	structures	875:884	arg1	Neu5Gc					929:934	Neu5Gc	929:934	Neu5Gc	929:934	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	5	102	theme	acid	1087:1090	arg1	content					1069:1075	the Neu5Gc content	1058:1075	the Neu5Gc content of sialic acid	1058:1090	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	5	102	theme	acid	1087:1090	arg1	%					1110:1110	<3%	1108:1110	<3%	1108:1110	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	5	102	theme	acid	1087:1090	arg1	negligible					1096:1105	negligible	1096:1105	negligible	1096:1105	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	4	103	theme	acid	920:923	arg1	%					908:908	75 %	905:908	75 % of sialic acid	905:923	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	4	103	theme	acid	920:923	arg1	acid					920:923	sialic acid	913:923	sialic acid	913:923	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	0	104	theme	NS0	21:23	arg1	cultures					38:45	NS0 and CHO cell cultures	21:45	cultures	38:45	Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies.					
33901620	0	105	theme	CHO	29:31	arg1	cultures					38:45	NS0 and CHO cell cultures	21:45	cultures	38:45	Strategic feeding of NS0 and CHO cell cultures to control glycan profiles and immunogenic epitopes of monoclonal antibodies.					
33901620	1	106	theme	lines	287:291	arg1	CHO					303:305	CHO	303:305	CHO	303:305	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	1	106	theme	lines	287:291	arg1	variety					271:277	a variety	269:277	a variety of cell lines including CHO and NS0	269:313	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	1	106	theme	lines	287:291	arg1	lines					287:291	cell lines	282:291	cell lines	282:291	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	1	106	theme	lines	287:291	arg1	NS0					311:313	NS0	311:313	NS0	311:313	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	8	107	gly	sialylation	1401:1411	arg1	Mab					1428:1430	the EG2-hFc Mab	1416:1430	the EG2-hFc Mab	1416:1430	We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.					
33901620	9	108	theme	Hu1D10	1593:1598	arg1	sialylation					1578:1588	sialylation	1578:1588	sialylation of Hu1D10	1578:1598	On the other hand, MG or MGU increased sialylation of Hu1D10 substantially (SI:0.72) with an increase that could be attributed predominantly to the formation of Neu5Ac rather than Neu5Gc.					
33901620	3	109	gly	sialylation	656:666	arg1	epitopes					720:727	these non-human glycan epitopes	697:727	these non-human glycan epitopes	697:727	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	3	110	theme	Mab	585:587	arg1	production					589:598	Mab production	585:598	Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes	585:727	We have looked at the effects of a commonly used media supplements of manganese, galactose and uridine (MGU) on Mab production from CHO and NS0 cells in enhancing galactosylation and sialylation as well as the generation of these non-human glycan epitopes.					
33901620	4	111	theme	MGU	752:754	arg1	supplement					756:765	the MGU supplement	748:765	the MGU supplement	748:765	In the absence of the MGU supplement, the humanized IgG1 antibody (Hu1D10) produced from NS0 cells showed a low level of mono- and di-sialylated structures (SI:0.09) of which 75 % of sialic acid was Neu5Gc.					
33901620	6	112	theme	Mabs	1249:1252	arg1	galactosylation					1225:1239	the galactosylation	1221:1239	the galactosylation of both Mabs	1221:1252	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	5	113	theme	sialylation	1031:1041	arg1	level					1022:1026	an equally low level	1007:1026	an equally low level of sialylation (SI: 0.12)	1007:1052	The chimeric human-llama Mab (EG2-hFc) produced from CHO cells showed an equally low level of sialylation (SI: 0.12) but the Neu5Gc content of sialic acid was negligible (<3%).					
33901620	6	114	dep	GI:0.78	1261:1267	arg1	to					1258:1259	to	1258:1259	to	1258:1259	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	2	115	theme	particular	319:328	arg1	concern					330:336	particular concern	319:336	particular concern	319:336	Of particular concern is the potential for generating non-human epitopes such as N-glycolylneuraminic acid (Neu5Gc) and Galα1-3 Gal that may be immunogenic.					
33901620	8	116	theme	MG	1452:1453	arg1	combination					1437:1447	a combination	1435:1447	a combination of MG	1435:1453	We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.					
33901620	6	117	from	0.81	1283:1286	arg1	EG2-hFc					1291:1297	EG2-hFc	1291:1297	EG2-hFc	1291:1297	Combinations of the MGU supplements added to the production cultures resulted in a substantial increase in the galactosylation of both Mabs (up to GI:0.78 in Hu1D10 and 0.81 in EG2-hFc).					
33901620	12	118	theme	fetal	2235:2239	arg1	FBS					2255:2257	FBS	2255:2257	FBS	2255:2257	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	12	118	theme	fetal	2235:2239	arg1	serum					2248:2252	fetal bovine serum	2235:2252	fetal bovine serum (FBS)	2235:2258	Supplementation of media with fetal bovine serum (FBS) increased the availability of free Neu5Ac which resulted in a significant increase in the sialylation of Hu1D10 from NS0 cells.					
33901620	13	119	theme	measured	2468:2475	arg1	acid					2484:2487	the measured sialic acid	2464:2487	the measured sialic acid from the Mab	2464:2500	This also resulted in a significant decrease in the proportion of Neu5Gc in the measured sialic acid from the Mab.					
33901620	1	120	theme	antibodies	232:241	arg1	manufacture					194:204	the consistent manufacture	179:204	the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0	179:313	The control of glycosylation profiles is essential to the consistent manufacture of therapeutic monoclonal antibodies that may be produced from a variety of cell lines including CHO and NS0.					
33901620	11	121	theme	NS0	2069:2071	arg1	cells					2073:2077	NS0 cells	2069:2077	NS0 cells	2069:2077	However, this effect not only increased the desirable beta 1-4 Gal linkage to GlcNAc but unfortunately in NS0 cells increased the formation of Galα1-3 Gal which was shown to increase x3 in the presence of combinations of the MGU supplements.					
33901620	8	122	theme	EG2-hFc	1420:1426	arg1	Mab					1428:1430	the EG2-hFc Mab	1416:1430	the EG2-hFc Mab	1416:1430	We observed a slight increase in sialylation of the EG2-hFc Mab by a combination of MG but it appeared that one of the components (uridine) was inhibitory to sialylation.					
33559609	4	0	theme	catalytic	693:701	arg1	residues					713:720	the two catalytic glutamate residues	685:720	the two catalytic glutamate residues present	685:728	It is the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present.					
33559609	10	1	theme	retaining	1538:1546	arg1	GHs					1548:1550	other retaining GHs	1532:1550	other retaining GHs in clan GH-A	1532:1563	The approach used to obtain the GEI presented here could easily be transposed to other retaining GHs in clan GH-A.					
33559609	0	2	theme	residues	97:104	arg1	involvement					68:78	the involvement	64:78	the involvement of two conserved residues in catalysis	64:117	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.					
33559609	5	3	with	complex	776:782	arg1	cellotriose					789:799	cellotriose	789:799	cellotriose	789:799	The structure of the variant RBcel1_E135A in complex with cellotriose is also reported, allowing a description of the entire binding cleft of RBcel1.					
33559609	10	4	theme	clan	1555:1558	arg1	GH-A					1560:1563	clan GH-A	1555:1563	clan GH-A	1555:1563	The approach used to obtain the GEI presented here could easily be transposed to other retaining GHs in clan GH-A.					
33559609	7	5	theme	structural	982:991	arg1	analysis					993:1000	The structural analysis	978:1000	The structural analysis	978:1000	The structural analysis revealed a significant movement of the nucleophilic glutamate residue during the reaction.					
33559609	9	6	theme	RBcel1_Y201F	1429:1440	arg1	variant					1442:1448	the RBcel1_Y201F variant	1425:1448	the RBcel1_Y201F variant	1425:1448	Moreover, a conserved tyrosine residue in the -1 subsite, Tyr201, plays a determinant role in both the glycosylation and deglycosylation steps, since the GEI was trapped in the RBcel1_Y201F variant.					
33559609	0	7	theme	conserved	87:95	arg1	residues					97:104	two conserved residues	83:104	two conserved residues	83:104	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.					
33559609	2	8	theme	glycosyl-enzyme	287:301	arg1	GEI					317:319	GEI	317:319	GEI	317:319	Studying reaction intermediates, such as the glycosyl-enzyme intermediate (GEI) and the Michaelis complex, could provide valuable information to better understand the molecular factors governing the catalytic mechanism.					
33559609	2	8	theme	glycosyl-enzyme	287:301	arg1	intermediate					303:314	the glycosyl-enzyme intermediate	283:314	the glycosyl-enzyme intermediate (GEI)	283:320	Studying reaction intermediates, such as the glycosyl-enzyme intermediate (GEI) and the Michaelis complex, could provide valuable information to better understand the molecular factors governing the catalytic mechanism.					
33559609	3	9	theme	transglycosylase	552:567	arg1	activity					569:576	transglycosylase activity	552:576	transglycosylase activity	552:576	Here, the GEI structure of RBcel1, an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity, is reported.					
33559609	4	10	theme	present	722:728	arg1	residues					713:720	the two catalytic glutamate residues	685:720	the two catalytic glutamate residues present	685:728	It is the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present.					
33559609	9	11	theme	determinant	1326:1336	arg1	role					1338:1341	a determinant role	1324:1341	a determinant role	1324:1341	Moreover, a conserved tyrosine residue in the -1 subsite, Tyr201, plays a determinant role in both the glycosylation and deglycosylation steps, since the GEI was trapped in the RBcel1_Y201F variant.					
33559609	8	12	theme	glutamate	1153:1161	arg1	crucial					1174:1180	crucial	1174:1180	crucial	1174:1180	Enzymatic assays indicated that, as expected, the acid/base glutamate residue is crucial for the glycosylation step and partly contributes to deglycosylation.					
33559609	8	12	theme	glutamate	1153:1161	arg1	residue					1163:1169	the acid/base glutamate residue	1139:1169	the acid/base glutamate residue	1139:1169	Enzymatic assays indicated that, as expected, the acid/base glutamate residue is crucial for the glycosylation step and partly contributes to deglycosylation.					
33559609	3	13	theme	family	532:537	arg1	RBcel1					489:494	RBcel1	489:494	RBcel1	489:494	Here, the GEI structure of RBcel1, an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity, is reported.					
33559609	3	13	theme	family	532:537	arg1	endo-1,4-β-glucanase					500:519	an endo-1,4-β-glucanase	497:519	an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity	497:576	Here, the GEI structure of RBcel1, an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity, is reported.					
33559609	5	14	theme	binding	856:862	arg1	cleft					864:868	the entire binding cleft	845:868	the entire binding cleft of RBcel1	845:878	The structure of the variant RBcel1_E135A in complex with cellotriose is also reported, allowing a description of the entire binding cleft of RBcel1.					
33559609	8	15	theme	glycosylation	1190:1202	arg1	step					1204:1207	the glycosylation step	1186:1207	the glycosylation step	1186:1207	Enzymatic assays indicated that, as expected, the acid/base glutamate residue is crucial for the glycosylation step and partly contributes to deglycosylation.					
33559609	3	16	theme	endowed	539:545	arg1	family					532:537	the GH5 family	524:537	the GH5 family endowed with transglycosylase activity	524:576	Here, the GEI structure of RBcel1, an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity, is reported.					
33559609	9	17	theme	tyrosine	1274:1281	arg1	residue					1283:1289	a conserved tyrosine residue	1262:1289	a conserved tyrosine residue in the -1 subsite	1262:1307	Moreover, a conserved tyrosine residue in the -1 subsite, Tyr201, plays a determinant role in both the glycosylation and deglycosylation steps, since the GEI was trapped in the RBcel1_Y201F variant.					
33559609	9	17	theme	tyrosine	1274:1281	arg1	Tyr201					1310:1315	Tyr201	1310:1315	Tyr201	1310:1315	Moreover, a conserved tyrosine residue in the -1 subsite, Tyr201, plays a determinant role in both the glycosylation and deglycosylation steps, since the GEI was trapped in the RBcel1_Y201F variant.					
33559609	5	18	theme	cleft	864:868	arg1	description					830:840	a description	828:840	a description of the entire binding cleft of RBcel1	828:878	The structure of the variant RBcel1_E135A in complex with cellotriose is also reported, allowing a description of the entire binding cleft of RBcel1.					
33559609	0	19	theme	hydrolase	10:18	arg1	family					20:25	Glycoside hydrolase family 5	0:27	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.	0:118	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.					
33559609	8	20	theme	Enzymatic	1093:1101	arg1	assays					1103:1108	Enzymatic assays	1093:1108	Enzymatic assays	1093:1108	Enzymatic assays indicated that, as expected, the acid/base glutamate residue is crucial for the glycosylation step and partly contributes to deglycosylation.					
33559609	0	21	theme	Glycoside	0:8	arg1	family					20:25	Glycoside hydrolase family 5	0:27	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.	0:118	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.					
33559609	5	22	from	RBcel1_E135A	760:771	arg1	complex					776:782	complex	776:782	complex with cellotriose	776:799	The structure of the variant RBcel1_E135A in complex with cellotriose is also reported, allowing a description of the entire binding cleft of RBcel1.					
33559609	4	23	theme	natural	656:662	arg1	oligosaccharide					664:678	a natural oligosaccharide	654:678	a natural oligosaccharide	654:678	It is the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present.					
33559609	3	24	theme	GEI	472:474	arg1	structure					476:484	the GEI structure	468:484	the GEI structure of RBcel1, an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity,	468:577	Here, the GEI structure of RBcel1, an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity, is reported.					
33559609	2	25	theme	catalytic	441:449	arg1	mechanism					451:459	the catalytic mechanism	437:459	the catalytic mechanism	437:459	Studying reaction intermediates, such as the glycosyl-enzyme intermediate (GEI) and the Michaelis complex, could provide valuable information to better understand the molecular factors governing the catalytic mechanism.					
33559609	10	26	from	GHs	1548:1550	arg1	GH-A					1560:1563	clan GH-A	1555:1563	clan GH-A	1555:1563	The approach used to obtain the GEI presented here could easily be transposed to other retaining GHs in clan GH-A.					
33559609	4	27	theme	GH5	623:625	arg1	enzyme					627:632	a GH5 enzyme	621:632	a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present	621:728	It is the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present.					
33559609	0	28	theme	structural	30:39	arg1	snapshots					41:49	structural snapshots	30:49	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.	0:118	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.					
33559609	6	29	theme	mechanism	967:975	arg1	snapshots					930:938	different snapshots	920:938	different snapshots of the double-displacement mechanism	920:975	Taken together, the structures deliver different snapshots of the double-displacement mechanism.					
33559609	9	30	theme	conserved	1264:1272	arg1	residue					1283:1289	a conserved tyrosine residue	1262:1289	a conserved tyrosine residue in the -1 subsite	1262:1307	Moreover, a conserved tyrosine residue in the -1 subsite, Tyr201, plays a determinant role in both the glycosylation and deglycosylation steps, since the GEI was trapped in the RBcel1_Y201F variant.					
33559609	9	30	theme	conserved	1264:1272	arg1	Tyr201					1310:1315	Tyr201	1310:1315	Tyr201	1310:1315	Moreover, a conserved tyrosine residue in the -1 subsite, Tyr201, plays a determinant role in both the glycosylation and deglycosylation steps, since the GEI was trapped in the RBcel1_Y201F variant.					
33559609	5	31	theme	RBcel1	873:878	arg1	cleft					864:868	the entire binding cleft	845:868	the entire binding cleft of RBcel1	845:878	The structure of the variant RBcel1_E135A in complex with cellotriose is also reported, allowing a description of the entire binding cleft of RBcel1.					
33559609	3	32	with	endowed	539:545	arg1	activity					569:576	transglycosylase activity	552:576	transglycosylase activity	552:576	Here, the GEI structure of RBcel1, an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity, is reported.					
33559609	6	33	theme	double-displacement	947:965	arg1	mechanism					967:975	the double-displacement mechanism	943:975	the double-displacement mechanism	943:975	Taken together, the structures deliver different snapshots of the double-displacement mechanism.					
33559609	0	34	from	involvement	68:78	arg1	catalysis					109:117	catalysis	109:117	catalysis	109:117	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.					
33559609	2	35	theme	molecular	409:417	arg1	factors					419:425	the molecular factors	405:425	the molecular factors governing the catalytic mechanism	405:459	Studying reaction intermediates, such as the glycosyl-enzyme intermediate (GEI) and the Michaelis complex, could provide valuable information to better understand the molecular factors governing the catalytic mechanism.					
33559609	9	36	theme	deglycosylation	1373:1387	arg1	steps					1389:1393	both the glycosylation and deglycosylation steps	1346:1393	steps	1389:1393	Moreover, a conserved tyrosine residue in the -1 subsite, Tyr201, plays a determinant role in both the glycosylation and deglycosylation steps, since the GEI was trapped in the RBcel1_Y201F variant.					
33559609	10	37	theme	other	1532:1536	arg1	GHs					1548:1550	other retaining GHs	1532:1550	other retaining GHs in clan GH-A	1532:1563	The approach used to obtain the GEI presented here could easily be transposed to other retaining GHs in clan GH-A.					
33559609	5	38	from	structure	735:743	arg1	complex					776:782	complex	776:782	complex with cellotriose	776:799	The structure of the variant RBcel1_E135A in complex with cellotriose is also reported, allowing a description of the entire binding cleft of RBcel1.					
33559609	7	39	theme	nucleophilic	1041:1052	arg1	residue					1064:1070	the nucleophilic glutamate residue	1037:1070	the nucleophilic glutamate residue	1037:1070	The structural analysis revealed a significant movement of the nucleophilic glutamate residue during the reaction.					
33559609	2	40	theme	valuable	363:370	arg1	information					372:382	valuable information	363:382	valuable information to better understand the molecular factors governing the catalytic mechanism	363:459	Studying reaction intermediates, such as the glycosyl-enzyme intermediate (GEI) and the Michaelis complex, could provide valuable information to better understand the molecular factors governing the catalytic mechanism.					
33559609	1	41	theme	glycoside	145:153	arg1	GHs					167:169	GHs	167:169	GHs	167:169	The ability of retaining glycoside hydrolases (GHs) to transglycosylate is inherent to the double-displacement mechanism.					
33559609	1	41	theme	glycoside	145:153	arg1	hydrolases					155:164	glycoside hydrolases	145:164	glycoside hydrolases (GHs)	145:170	The ability of retaining glycoside hydrolases (GHs) to transglycosylate is inherent to the double-displacement mechanism.					
33559609	4	42	theme	glutamate	703:711	arg1	residues					713:720	the two catalytic glutamate residues	685:720	the two catalytic glutamate residues present	685:728	It is the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present.					
33559609	7	43	theme	significant	1013:1023	arg1	movement					1025:1032	a significant movement	1011:1032	a significant movement of the nucleophilic glutamate residue during the reaction	1011:1090	The structural analysis revealed a significant movement of the nucleophilic glutamate residue during the reaction.					
33559609	7	44	theme	glutamate	1054:1062	arg1	residue					1064:1070	the nucleophilic glutamate residue	1037:1070	the nucleophilic glutamate residue	1037:1070	The structural analysis revealed a significant movement of the nucleophilic glutamate residue during the reaction.					
33559609	5	45	from	complex	776:782	arg1	structure					735:743	The structure	731:743	The structure of the variant RBcel1_E135A in complex with cellotriose	731:799	The structure of the variant RBcel1_E135A in complex with cellotriose is also reported, allowing a description of the entire binding cleft of RBcel1.					
33559609	2	46	theme	Michaelis	330:338	arg1	complex					340:346	the Michaelis complex	326:346	the Michaelis complex	326:346	Studying reaction intermediates, such as the glycosyl-enzyme intermediate (GEI) and the Michaelis complex, could provide valuable information to better understand the molecular factors governing the catalytic mechanism.					
33559609	1	47	theme	double-displacement	211:229	arg1	mechanism					231:239	the double-displacement mechanism	207:239	the double-displacement mechanism	207:239	The ability of retaining glycoside hydrolases (GHs) to transglycosylate is inherent to the double-displacement mechanism.					
33559609	7	48	theme	residue	1064:1070	arg1	movement					1025:1032	a significant movement	1011:1032	a significant movement of the nucleophilic glutamate residue during the reaction	1011:1090	The structural analysis revealed a significant movement of the nucleophilic glutamate residue during the reaction.					
33559609	0	49	dep	family	20:25	arg1	snapshots					41:49	structural snapshots	30:49	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.	0:118	Glycoside hydrolase family 5: structural snapshots highlighting the involvement of two conserved residues in catalysis.					
33559609	8	50	theme	acid/base	1143:1151	arg1	crucial					1174:1180	crucial	1174:1180	crucial	1174:1180	Enzymatic assays indicated that, as expected, the acid/base glutamate residue is crucial for the glycosylation step and partly contributes to deglycosylation.					
33559609	8	50	theme	acid/base	1143:1151	arg1	residue					1163:1169	the acid/base glutamate residue	1139:1169	the acid/base glutamate residue	1139:1169	Enzymatic assays indicated that, as expected, the acid/base glutamate residue is crucial for the glycosylation step and partly contributes to deglycosylation.					
33559609	3	51	theme	RBcel1	489:494	arg1	structure					476:484	the GEI structure	468:484	the GEI structure of RBcel1, an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity,	468:577	Here, the GEI structure of RBcel1, an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity, is reported.					
33559609	4	52	theme	first	602:606	arg1	structure					608:616	the first structure	598:616	the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present	598:728	It is the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present.					
33559609	4	52	theme	first	602:606	arg1	It					592:593	It	592:593	It	592:593	It is the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present.					
33559609	5	53	theme	variant	752:758	arg1	RBcel1_E135A					760:771	the variant RBcel1_E135A	748:771	the variant RBcel1_E135A in complex with cellotriose	748:799	The structure of the variant RBcel1_E135A in complex with cellotriose is also reported, allowing a description of the entire binding cleft of RBcel1.					
33559609	5	54	theme	entire	849:854	arg1	cleft					864:868	the entire binding cleft	845:868	the entire binding cleft of RBcel1	845:878	The structure of the variant RBcel1_E135A in complex with cellotriose is also reported, allowing a description of the entire binding cleft of RBcel1.					
33559609	4	55	theme	enzyme	627:632	arg1	structure					608:616	the first structure	598:616	the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present	598:728	It is the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present.					
33559609	4	55	theme	enzyme	627:632	arg1	It					592:593	It	592:593	It	592:593	It is the first structure of a GH5 enzyme covalently bound to a natural oligosaccharide with the two catalytic glutamate residues present.					
33559609	5	56	theme	RBcel1_E135A	760:771	arg1	structure					735:743	The structure	731:743	The structure of the variant RBcel1_E135A in complex with cellotriose	731:799	The structure of the variant RBcel1_E135A in complex with cellotriose is also reported, allowing a description of the entire binding cleft of RBcel1.					
33559609	6	57	theme	different	920:928	arg1	snapshots					930:938	different snapshots	920:938	different snapshots of the double-displacement mechanism	920:975	Taken together, the structures deliver different snapshots of the double-displacement mechanism.					
33559609	2	58	theme	reaction	251:258	arg1	complex					340:346	the Michaelis complex	326:346	the Michaelis complex	326:346	Studying reaction intermediates, such as the glycosyl-enzyme intermediate (GEI) and the Michaelis complex, could provide valuable information to better understand the molecular factors governing the catalytic mechanism.					
33559609	2	58	theme	reaction	251:258	arg1	intermediates					260:272	reaction intermediates	251:272	reaction intermediates	251:272	Studying reaction intermediates, such as the glycosyl-enzyme intermediate (GEI) and the Michaelis complex, could provide valuable information to better understand the molecular factors governing the catalytic mechanism.					
33559609	2	58	theme	reaction	251:258	arg1	intermediate					303:314	the glycosyl-enzyme intermediate	283:314	the glycosyl-enzyme intermediate (GEI)	283:320	Studying reaction intermediates, such as the glycosyl-enzyme intermediate (GEI) and the Michaelis complex, could provide valuable information to better understand the molecular factors governing the catalytic mechanism.					
33559609	3	59	theme	GH5	528:530	arg1	family					532:537	the GH5 family	524:537	the GH5 family endowed with transglycosylase activity	524:576	Here, the GEI structure of RBcel1, an endo-1,4-β-glucanase of the GH5 family endowed with transglycosylase activity, is reported.					
33559609	9	60	from	residue	1283:1289	arg1	subsite					1301:1307	the -1 subsite	1294:1307	the -1 subsite	1294:1307	Moreover, a conserved tyrosine residue in the -1 subsite, Tyr201, plays a determinant role in both the glycosylation and deglycosylation steps, since the GEI was trapped in the RBcel1_Y201F variant.					
34369795	2	0	theme	host	327:330	arg1	cell					332:335	the host cell	323:335	the host cell	323:335	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	7	1	from	distribution	1075:1086	arg1	protein					1113:1119	the coronaviruses' S1 protein	1091:1119	the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry	1091:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	7	2	theme	uncharted	1032:1040	arg1	territory					1042:1050	the uncharted territory	1028:1050	the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry	1028:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	1	3	theme	COVID-19	141:148	arg1	pandemic					150:157	COVID-19 pandemic	141:157	COVID-19 pandemic	141:157	The emergence of COVID-19 pandemic has engaged the scientific community around the globe in the rapid development of effective therapeutics and vaccines.					
34369795	2	4	from	role	299:302	arg1	invasion					311:318	the invasion	307:318	the invasion of the host cell	307:335	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	0	5	theme	MERS-CoV	86:93	arg1	Glycoprotein					68:79	Spike Glycoprotein 1	62:81	Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2	62:121	Direct Comparison of N-Glycans and Their Isomers Derived from Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2.					
34369795	2	6	from	targets	381:387	arg1	studies					398:404	these studies	392:404	these studies	392:404	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	2	7	gly	glycoprotein	348:359	arg1	glycoprotein					348:359	spike (S) glycoprotein	338:359	spike (S) glycoprotein	338:359	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	8	8	theme	isomeric	1271:1278	arg1	glycans					1280:1286	isomeric glycans	1271:1286	isomeric glycans	1271:1286	We believe that our data would facilitate future investigations to study the role of isomeric glycans in coronavirus viral pathogenesis.					
34369795	1	9	theme	effective	241:249	arg1	therapeutics					251:262	effective therapeutics	241:262	effective therapeutics	241:262	The emergence of COVID-19 pandemic has engaged the scientific community around the globe in the rapid development of effective therapeutics and vaccines.					
34369795	6	10	theme	acute	874:878	arg1	coronaviruses					852:864	other coronaviruses	846:864	other coronaviruses	846:864	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	10	theme	acute	874:878	arg1	coronavirus					901:911	severe acute respiratory syndrome coronavirus 1	867:913	severe acute respiratory syndrome coronavirus 1	867:913	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	7	11	from	protein	1113:1119	arg1	territory					1042:1050	the uncharted territory	1028:1050	the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry	1028:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	4	12	gly	glycosylated	575:586	arg1	protein					588:594	a heavily glycosylated protein	565:594	a heavily glycosylated protein	565:594	Being a heavily glycosylated protein, numerous studies have investigated its glycan composition.					
34369795	3	13	theme	S	429:429	arg1	protein					431:437	the S protein	425:437	the S protein (S1 protein)	425:450	The S1 subunit of the S protein (S1 protein) accommodates the receptor-binding domain, which enables the initial binding of the virus to the host cell.					
34369795	3	13	theme	S	429:429	arg1	protein					443:449	S1 protein	440:449	S1 protein	440:449	The S1 subunit of the S protein (S1 protein) accommodates the receptor-binding domain, which enables the initial binding of the virus to the host cell.					
34369795	1	14	theme	therapeutics	251:262	arg1	development					226:236	the rapid development	216:236	the rapid development of effective therapeutics and vaccines	216:275	The emergence of COVID-19 pandemic has engaged the scientific community around the globe in the rapid development of effective therapeutics and vaccines.					
34369795	0	15	theme	SARS-CoV-1	96:105	arg1	Glycoprotein					68:79	Spike Glycoprotein 1	62:81	Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2	62:121	Direct Comparison of N-Glycans and Their Isomers Derived from Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2.					
34369795	1	16	theme	pandemic	150:157	arg1	emergence					128:136	The emergence	124:136	The emergence of COVID-19 pandemic	124:157	The emergence of COVID-19 pandemic has engaged the scientific community around the globe in the rapid development of effective therapeutics and vaccines.					
34369795	6	17	theme	syndrome	892:899	arg1	coronaviruses					852:864	other coronaviruses	846:864	other coronaviruses	846:864	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	17	theme	syndrome	892:899	arg1	coronavirus					901:911	severe acute respiratory syndrome coronavirus 1	867:913	severe acute respiratory syndrome coronavirus 1	867:913	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	8	18	from	role	1263:1266	arg1	pathogenesis					1309:1320	coronavirus viral pathogenesis	1291:1320	coronavirus viral pathogenesis	1291:1320	We believe that our data would facilitate future investigations to study the role of isomeric glycans in coronavirus viral pathogenesis.					
34369795	0	19	theme	Direct	0:5	arg1	Comparison					7:16	Direct Comparison	0:16	Direct Comparison of N-Glycans	0:29	Direct Comparison of N-Glycans and Their Isomers Derived from Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2.					
34369795	2	20	theme	S	345:345	arg1	glycoprotein					348:359	spike (S) glycoprotein	338:359	spike (S) glycoprotein	338:359	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	5	21	theme	glycan	712:717	arg1	distribution					719:730	the isomeric glycan distribution	699:730	the isomeric glycan distribution of this protein	699:746	However, none of the studies have explored the isomeric glycan distribution of this protein.					
34369795	8	22	theme	viral	1303:1307	arg1	pathogenesis					1309:1320	coronavirus viral pathogenesis	1291:1320	coronavirus viral pathogenesis	1291:1320	We believe that our data would facilitate future investigations to study the role of isomeric glycans in coronavirus viral pathogenesis.					
34369795	4	23	theme	glycosylated	575:586	arg1	protein					588:594	a heavily glycosylated protein	565:594	a heavily glycosylated protein	565:594	Being a heavily glycosylated protein, numerous studies have investigated its glycan composition.					
34369795	2	24	theme	targets	381:387	arg1	targets					381:387	the major targets	371:387	the major targets in these studies	371:404	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	2	24	theme	targets	381:387	arg1	one					364:366	one	364:366	one	364:366	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	6	25	theme	respiratory	880:890	arg1	coronaviruses					852:864	other coronaviruses	846:864	other coronaviruses	846:864	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	25	theme	respiratory	880:890	arg1	coronavirus					901:911	severe acute respiratory syndrome coronavirus 1	867:913	severe acute respiratory syndrome coronavirus 1	867:913	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	2	26	theme	crucial	291:297	arg1	role					299:302	its crucial role	287:302	its crucial role in the invasion of the host cell	287:335	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	2	27	theme	spike	338:342	arg1	glycoprotein					348:359	spike (S) glycoprotein	338:359	spike (S) glycoprotein	338:359	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	0	28	theme	N-Glycans	21:29	arg1	Isomers					41:47	Their Isomers	35:47	Their Isomers	35:47	Direct Comparison of N-Glycans and Their Isomers Derived from Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2.					
34369795	0	28	theme	N-Glycans	21:29	arg1	Comparison					7:16	Direct Comparison	0:16	Direct Comparison of N-Glycans	0:29	Direct Comparison of N-Glycans and Their Isomers Derived from Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2.					
34369795	1	29	theme	vaccines	268:275	arg1	development					226:236	the rapid development	216:236	the rapid development of effective therapeutics and vaccines	216:275	The emergence of COVID-19 pandemic has engaged the scientific community around the globe in the rapid development of effective therapeutics and vaccines.					
34369795	3	30	theme	receptor-binding	469:484	arg1	domain					486:491	the receptor-binding domain	465:491	the receptor-binding domain	465:491	The S1 subunit of the S protein (S1 protein) accommodates the receptor-binding domain, which enables the initial binding of the virus to the host cell.					
34369795	1	31	theme	scientific	175:184	arg1	community					186:194	the scientific community	171:194	the scientific community around the globe in the rapid development of effective therapeutics and vaccines	171:275	The emergence of COVID-19 pandemic has engaged the scientific community around the globe in the rapid development of effective therapeutics and vaccines.					
34369795	8	32	theme	coronavirus	1291:1301	arg1	pathogenesis					1309:1320	coronavirus viral pathogenesis	1291:1320	coronavirus viral pathogenesis	1291:1320	We believe that our data would facilitate future investigations to study the role of isomeric glycans in coronavirus viral pathogenesis.					
34369795	6	33	theme	severe	867:872	arg1	coronaviruses					852:864	other coronaviruses	846:864	other coronaviruses	846:864	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	33	theme	severe	867:872	arg1	coronavirus					901:911	severe acute respiratory syndrome coronavirus 1	867:913	severe acute respiratory syndrome coronavirus 1	867:913	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	3	34	theme	protein	431:437	arg1	subunit					414:420	The S1 subunit	407:420	The S1 subunit of the S protein (S1 protein)	407:450	The S1 subunit of the S protein (S1 protein) accommodates the receptor-binding domain, which enables the initial binding of the virus to the host cell.					
34369795	7	35	theme	glycan	1068:1073	arg1	distribution					1075:1086	the isomeric glycan distribution	1055:1086	the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry	1055:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	7	36	theme	S1	1110:1111	arg1	protein					1113:1119	the coronaviruses' S1 protein	1091:1119	the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry	1091:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	6	37	theme	glycan	776:781	arg1	distribution					783:794	this isomeric glycan distribution	762:794	this isomeric glycan distribution	762:794	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	3	38	theme	initial	512:518	arg1	binding					520:526	the initial binding	508:526	the initial binding of the virus to the host cell	508:556	The S1 subunit of the S protein (S1 protein) accommodates the receptor-binding domain, which enables the initial binding of the virus to the host cell.					
34369795	7	39	theme	distribution	1075:1086	arg1	territory					1042:1050	the uncharted territory	1028:1050	the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry	1028:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	6	40	theme	coronaviruses	852:864	arg1	proteins					834:841	S1 proteins	831:841	S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics	831:1005	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	0	41	theme	SARS-CoV-2	112:121	arg1	Glycoprotein					68:79	Spike Glycoprotein 1	62:81	Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2	62:121	Direct Comparison of N-Glycans and Their Isomers Derived from Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2.					
34369795	6	42	theme	isomeric	767:774	arg1	distribution					783:794	this isomeric glycan distribution	762:794	this isomeric glycan distribution	762:794	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	2	43	theme	major	375:379	arg1	targets					381:387	the major targets	371:387	the major targets in these studies	371:404	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	8	44	theme	future	1228:1233	arg1	investigations					1235:1248	future investigations	1228:1248	future investigations	1228:1248	We believe that our data would facilitate future investigations to study the role of isomeric glycans in coronavirus viral pathogenesis.					
34369795	6	45	theme	other	846:850	arg1	coronavirus					952:962	Middle East respiratory syndrome coronavirus	919:962	Middle East respiratory syndrome coronavirus	919:962	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	45	theme	other	846:850	arg1	coronaviruses					852:864	other coronaviruses	846:864	other coronaviruses	846:864	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	45	theme	other	846:850	arg1	coronavirus					901:911	severe acute respiratory syndrome coronavirus 1	867:913	severe acute respiratory syndrome coronavirus 1	867:913	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	3	46	theme	host	548:551	arg1	cell					553:556	the host cell	544:556	the host cell	544:556	The S1 subunit of the S protein (S1 protein) accommodates the receptor-binding domain, which enables the initial binding of the virus to the host cell.					
34369795	5	47	theme	isomeric	703:710	arg1	distribution					719:730	the isomeric glycan distribution	699:730	the isomeric glycan distribution of this protein	699:746	However, none of the studies have explored the isomeric glycan distribution of this protein.					
34369795	7	48	from	territory	1042:1050	arg1	protein					1113:1119	the coronaviruses' S1 protein	1091:1119	the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry	1091:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	7	49	theme	liquid	1127:1132	arg1	chromatography					1134:1147	liquid chromatography	1127:1147	liquid chromatography coupled to tandem mass spectrometry	1127:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	6	50	theme	respiratory	931:941	arg1	coronavirus					952:962	Middle East respiratory syndrome coronavirus	919:962	Middle East respiratory syndrome coronavirus	919:962	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	50	theme	respiratory	931:941	arg1	coronaviruses					852:864	other coronaviruses	846:864	other coronaviruses	846:864	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	7	51	theme	isomeric	1059:1066	arg1	distribution					1075:1086	the isomeric glycan distribution	1055:1086	the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry	1055:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	3	52	theme	virus	535:539	arg1	binding					520:526	the initial binding	508:526	the initial binding of the virus to the host cell	508:556	The S1 subunit of the S protein (S1 protein) accommodates the receptor-binding domain, which enables the initial binding of the virus to the host cell.					
34369795	1	53	from	community	186:194	arg1	development					226:236	the rapid development	216:236	the rapid development of effective therapeutics and vaccines	216:275	The emergence of COVID-19 pandemic has engaged the scientific community around the globe in the rapid development of effective therapeutics and vaccines.					
34369795	3	54	theme	S1	411:412	arg1	subunit					414:420	The S1 subunit	407:420	The S1 subunit of the S protein (S1 protein)	407:450	The S1 subunit of the S protein (S1 protein) accommodates the receptor-binding domain, which enables the initial binding of the virus to the host cell.					
34369795	4	55	theme	numerous	597:604	arg1	studies					606:612	numerous studies	597:612	numerous studies	597:612	Being a heavily glycosylated protein, numerous studies have investigated its glycan composition.					
34369795	6	56	theme	syndrome	943:950	arg1	coronavirus					952:962	Middle East respiratory syndrome coronavirus	919:962	Middle East respiratory syndrome coronavirus	919:962	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	56	theme	syndrome	943:950	arg1	coronaviruses					852:864	other coronaviruses	846:864	other coronaviruses	846:864	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	8	57	theme	glycans	1280:1286	arg1	role					1263:1266	the role	1259:1266	the role of isomeric glycans in coronavirus viral pathogenesis	1259:1320	We believe that our data would facilitate future investigations to study the role of isomeric glycans in coronavirus viral pathogenesis.					
34369795	3	58	theme	S1	440:441	arg1	protein					431:437	the S protein	425:437	the S protein (S1 protein)	425:450	The S1 subunit of the S protein (S1 protein) accommodates the receptor-binding domain, which enables the initial binding of the virus to the host cell.					
34369795	3	58	theme	S1	440:441	arg1	protein					443:449	S1 protein	440:449	S1 protein	440:449	The S1 subunit of the S protein (S1 protein) accommodates the receptor-binding domain, which enables the initial binding of the virus to the host cell.					
34369795	4	59	theme	glycan	636:641	arg1	composition					643:653	its glycan composition	632:653	its glycan composition	632:653	Being a heavily glycosylated protein, numerous studies have investigated its glycan composition.					
34369795	0	60	theme	Spike	62:66	arg1	Glycoprotein					68:79	Spike Glycoprotein 1	62:81	Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2	62:121	Direct Comparison of N-Glycans and Their Isomers Derived from Spike Glycoprotein 1 of MERS-CoV, SARS-CoV-1, and SARS-CoV-2.					
34369795	5	61	theme	protein	740:746	arg1	distribution					719:730	the isomeric glycan distribution	699:730	the isomeric glycan distribution of this protein	699:746	However, none of the studies have explored the isomeric glycan distribution of this protein.					
34369795	6	62	theme	past	992:995	arg1	epidemics					997:1005	past epidemics	992:1005	past epidemics	992:1005	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	63	theme	East	926:929	arg1	coronavirus					952:962	Middle East respiratory syndrome coronavirus	919:962	Middle East respiratory syndrome coronavirus	919:962	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	63	theme	East	926:929	arg1	coronaviruses					852:864	other coronaviruses	846:864	other coronaviruses	846:864	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	7	64	theme	tandem	1160:1165	arg1	spectrometry					1172:1183	tandem mass spectrometry	1160:1183	tandem mass spectrometry	1160:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	6	65	theme	S1	831:832	arg1	proteins					834:841	S1 proteins	831:841	S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics	831:1005	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	66	theme	Middle	919:924	arg1	coronavirus					952:962	Middle East respiratory syndrome coronavirus	919:962	Middle East respiratory syndrome coronavirus	919:962	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	6	66	theme	Middle	919:924	arg1	coronaviruses					852:864	other coronaviruses	846:864	other coronaviruses	846:864	Furthermore, this isomeric glycan distribution has never been compared to that in S1 proteins of other coronaviruses, severe acute respiratory syndrome coronavirus 1 and Middle East respiratory syndrome coronavirus, which were responsible for past epidemics.					
34369795	2	67	theme	cell	332:335	arg1	invasion					311:318	the invasion	307:318	the invasion of the host cell	307:335	Owing to its crucial role in the invasion of the host cell, spike (S) glycoprotein is one of the major targets in these studies.					
34369795	7	68	theme	mass	1167:1170	arg1	spectrometry					1172:1183	tandem mass spectrometry	1160:1183	tandem mass spectrometry	1160:1183	This study explores the uncharted territory of the isomeric glycan distribution in the coronaviruses' S1 protein using liquid chromatography coupled to tandem mass spectrometry.					
34369795	1	69	theme	rapid	220:224	arg1	development					226:236	the rapid development	216:236	the rapid development of effective therapeutics and vaccines	216:275	The emergence of COVID-19 pandemic has engaged the scientific community around the globe in the rapid development of effective therapeutics and vaccines.					
34043942	5	0	theme	mass	788:791	arg1	spectrometry					793:804	mass spectrometry	788:804	mass spectrometry	788:804	We demonstrated the enzymatic incorporation of glucosamine into glycogen by glycogen synthase, and the release by glycogen phosphorylase by biochemical and structural methodologies, in primary astrocytes, and in vivo by isotopic tracing and mass spectrometry.					
34043942	6	1	theme	free	942:945	arg1	pools					947:951	free pools	942:951	free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation	942:1013	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	7	2	theme	system	1184:1189	arg1	diseases					1152:1159	multiple human diseases	1137:1159	multiple human diseases of the central nervous system	1137:1189	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	7	3	theme	protein	1090:1096	arg1	glycosylation					1098:1110	protein glycosylation	1090:1110	protein glycosylation	1090:1110	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	1	4	theme	diseases	150:157	arg1	defects					104:110	Glycosylation defects	90:110	Glycosylation defects	90:110	Glycosylation defects are a hallmark of many nervous system diseases.					
34043942	1	4	theme	diseases	150:157	arg1	hallmark					118:125	a hallmark	116:125	a hallmark of many nervous system diseases	116:157	Glycosylation defects are a hallmark of many nervous system diseases.					
34043942	0	5	theme	protein	67:73	arg1	glycosylation					75:87	protein glycosylation	67:87	protein glycosylation	67:87	Brain glycogen serves as a critical glucosamine cache required for protein glycosylation.					
34043942	2	6	theme	metabolic	187:195	arg1	basis					197:201	the molecular and metabolic basis	169:201	the molecular and metabolic basis for this pathology	169:220	However, the molecular and metabolic basis for this pathology is not fully understood.					
34043942	6	7	theme	storage	842:848	arg1	diseases					850:857	glycogen storage diseases	833:857	glycogen storage diseases	833:857	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	7	8	theme	nervous	1176:1182	arg1	system					1184:1189	the central nervous system	1164:1189	the central nervous system	1164:1189	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	6	9	theme	brain	889:893	arg1	metabolism					904:913	brain glycogen metabolism	889:913	brain glycogen metabolism	889:913	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	7	10	theme	central	1168:1174	arg1	system					1184:1189	the central nervous system	1164:1189	the central nervous system	1164:1189	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	5	11	theme	glycogen	623:630	arg1	synthase					632:639	glycogen synthase	623:639	glycogen synthase	623:639	We demonstrated the enzymatic incorporation of glucosamine into glycogen by glycogen synthase, and the release by glycogen phosphorylase by biochemical and structural methodologies, in primary astrocytes, and in vivo by isotopic tracing and mass spectrometry.					
34043942	6	12	theme	global	922:927	arg1	decreases					929:937	global decreases	922:937	global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation	922:1013	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	5	13	theme	biochemical	687:697	arg1	methodologies					714:726	biochemical and structural methodologies	687:726	biochemical and structural methodologies	687:726	We demonstrated the enzymatic incorporation of glucosamine into glycogen by glycogen synthase, and the release by glycogen phosphorylase by biochemical and structural methodologies, in primary astrocytes, and in vivo by isotopic tracing and mass spectrometry.					
34043942	5	14	theme	isotopic	767:774	arg1	tracing					776:782	isotopic tracing	767:782	isotopic tracing	767:782	We demonstrated the enzymatic incorporation of glucosamine into glycogen by glycogen synthase, and the release by glycogen phosphorylase by biochemical and structural methodologies, in primary astrocytes, and in vivo by isotopic tracing and mass spectrometry.					
34043942	6	15	theme	metabolism	904:913	arg1	disruption					875:884	disruption	875:884	disruption of brain glycogen metabolism	875:913	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	6	16	theme	mouse	817:821	arg1	models					823:828	two mouse models	813:828	two mouse models of glycogen storage diseases	813:857	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	6	17	theme	glycogen	895:902	arg1	metabolism					904:913	brain glycogen metabolism	889:913	brain glycogen metabolism	889:913	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	7	18	theme	human	1146:1150	arg1	diseases					1152:1159	multiple human diseases	1137:1159	multiple human diseases of the central nervous system	1137:1189	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	5	19	theme	glucosamine	594:604	arg1	incorporation					577:589	the enzymatic incorporation	563:589	the enzymatic incorporation of glucosamine into glycogen	563:618	We demonstrated the enzymatic incorporation of glucosamine into glycogen by glycogen synthase, and the release by glycogen phosphorylase by biochemical and structural methodologies, in primary astrocytes, and in vivo by isotopic tracing and mass spectrometry.					
34043942	5	20	theme	structural	703:712	arg1	methodologies					714:726	biochemical and structural methodologies	687:726	biochemical and structural methodologies	687:726	We demonstrated the enzymatic incorporation of glucosamine into glycogen by glycogen synthase, and the release by glycogen phosphorylase by biochemical and structural methodologies, in primary astrocytes, and in vivo by isotopic tracing and mass spectrometry.					
34043942	0	21	theme	Brain	0:4	arg1	glycogen					6:13	Brain glycogen	0:13	Brain glycogen	0:13	Brain glycogen serves as a critical glucosamine cache required for protein glycosylation.					
34043942	0	21	theme	Brain	0:4	arg1	cache					48:52	a critical glucosamine cache	25:52	a critical glucosamine cache required for protein glycosylation	25:87	Brain glycogen serves as a critical glucosamine cache required for protein glycosylation.					
34043942	1	22	theme	Glycosylation	90:102	arg1	defects					104:110	Glycosylation defects	90:110	Glycosylation defects	90:110	Glycosylation defects are a hallmark of many nervous system diseases.					
34043942	1	22	theme	Glycosylation	90:102	arg1	hallmark					118:125	a hallmark	116:125	a hallmark of many nervous system diseases	116:157	Glycosylation defects are a hallmark of many nervous system diseases.					
34043942	3	23	theme	glucosamine	350:360	arg1	metabolism					362:371	glucosamine metabolism	350:371	glucosamine metabolism through glycogenolysis	350:394	In this study, we found that N-linked protein glycosylation in the brain is metabolically channeled to glucosamine metabolism through glycogenolysis.					
34043942	7	24	from	roles	1063:1067	arg1	glycosylation					1098:1110	protein glycosylation	1090:1110	protein glycosylation	1090:1110	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	6	25	theme	glycosylation	1001:1013	arg1	pools					947:951	free pools	942:951	free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation	942:1013	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	5	26	theme	primary	732:738	arg1	astrocytes					740:749	primary astrocytes	732:749	primary astrocytes	732:749	We demonstrated the enzymatic incorporation of glucosamine into glycogen by glycogen synthase, and the release by glycogen phosphorylase by biochemical and structural methodologies, in primary astrocytes, and in vivo by isotopic tracing and mass spectrometry.					
34043942	2	27	theme	molecular	173:181	arg1	basis					197:201	the molecular and metabolic basis	169:201	the molecular and metabolic basis for this pathology	169:220	However, the molecular and metabolic basis for this pathology is not fully understood.					
34043942	0	28	theme	critical	27:34	arg1	glycogen					6:13	Brain glycogen	0:13	Brain glycogen	0:13	Brain glycogen serves as a critical glucosamine cache required for protein glycosylation.					
34043942	0	28	theme	critical	27:34	arg1	cache					48:52	a critical glucosamine cache	25:52	a critical glucosamine cache required for protein glycosylation	25:87	Brain glycogen serves as a critical glucosamine cache required for protein glycosylation.					
34043942	6	29	theme	protein	993:999	arg1	glycosylation					1001:1013	N-linked protein glycosylation	984:1013	N-linked protein glycosylation	984:1013	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	7	30	theme	direct	1117:1122	arg1	relevance					1124:1132	direct relevance	1117:1132	direct relevance to multiple human diseases of the central nervous system	1117:1189	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	7	31	theme	multiple	1137:1144	arg1	diseases					1152:1159	multiple human diseases	1137:1159	multiple human diseases of the central nervous system	1137:1189	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	6	32	theme	N-linked	984:991	arg1	glycosylation					1001:1013	N-linked protein glycosylation	984:1013	N-linked protein glycosylation	984:1013	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	4	33	theme	glycogen	464:471	arg1	glucosamine					416:426	glucosamine	416:426	glucosamine	416:426	We discovered that glucosamine is an abundant constituent of brain glycogen, which functions as a glucosamine reservoir for multiple glycoconjugates.					
34043942	4	33	theme	glycogen	464:471	arg1	reservoir					507:515	a glucosamine reservoir	493:515	a glucosamine reservoir for multiple glycoconjugates	493:544	We discovered that glucosamine is an abundant constituent of brain glycogen, which functions as a glucosamine reservoir for multiple glycoconjugates.					
34043942	4	33	theme	glycogen	464:471	arg1	constituent					443:453	an abundant constituent	431:453	an abundant constituent	431:453	We discovered that glucosamine is an abundant constituent of brain glycogen, which functions as a glucosamine reservoir for multiple glycoconjugates.					
34043942	7	34	theme	brain	1072:1076	arg1	glycogen					1078:1085	brain glycogen	1072:1085	brain glycogen	1072:1085	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	4	35	theme	multiple	521:528	arg1	glycoconjugates					530:544	multiple glycoconjugates	521:544	multiple glycoconjugates	521:544	We discovered that glucosamine is an abundant constituent of brain glycogen, which functions as a glucosamine reservoir for multiple glycoconjugates.					
34043942	3	36	theme	N-linked	276:283	arg1	glycosylation					293:305	N-linked protein glycosylation	276:305	N-linked protein glycosylation in the brain	276:318	In this study, we found that N-linked protein glycosylation in the brain is metabolically channeled to glucosamine metabolism through glycogenolysis.					
34043942	4	37	theme	brain	458:462	arg1	glycogen					464:471	brain glycogen	458:471	brain glycogen	458:471	We discovered that glucosamine is an abundant constituent of brain glycogen, which functions as a glucosamine reservoir for multiple glycoconjugates.					
34043942	7	38	theme	glycogen	1078:1085	arg1	roles					1063:1067	fundamental biological roles	1040:1067	fundamental biological roles of brain glycogen in protein glycosylation	1040:1110	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	0	39	theme	glucosamine	36:46	arg1	glycogen					6:13	Brain glycogen	0:13	Brain glycogen	0:13	Brain glycogen serves as a critical glucosamine cache required for protein glycosylation.					
34043942	0	39	theme	glucosamine	36:46	arg1	cache					48:52	a critical glucosamine cache	25:52	a critical glucosamine cache required for protein glycosylation	25:87	Brain glycogen serves as a critical glucosamine cache required for protein glycosylation.					
34043942	6	40	theme	UDP-N-acetylglucosamine	956:978	arg1	pools					947:951	free pools	942:951	free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation	942:1013	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	3	41	theme	protein	285:291	arg1	glycosylation					293:305	N-linked protein glycosylation	276:305	N-linked protein glycosylation in the brain	276:318	In this study, we found that N-linked protein glycosylation in the brain is metabolically channeled to glucosamine metabolism through glycogenolysis.					
34043942	3	42	from	glycosylation	293:305	arg1	brain					314:318	the brain	310:318	the brain	310:318	In this study, we found that N-linked protein glycosylation in the brain is metabolically channeled to glucosamine metabolism through glycogenolysis.					
34043942	5	43	theme	enzymatic	567:575	arg1	incorporation					577:589	the enzymatic incorporation	563:589	the enzymatic incorporation of glucosamine into glycogen	563:618	We demonstrated the enzymatic incorporation of glucosamine into glycogen by glycogen synthase, and the release by glycogen phosphorylase by biochemical and structural methodologies, in primary astrocytes, and in vivo by isotopic tracing and mass spectrometry.					
34043942	6	44	theme	glycogen	833:840	arg1	diseases					850:857	glycogen storage diseases	833:857	glycogen storage diseases	833:857	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	3	45	link	N-linked	276:283	arg1	glycosylation					293:305	N-linked protein glycosylation	276:305	N-linked protein glycosylation in the brain	276:318	In this study, we found that N-linked protein glycosylation in the brain is metabolically channeled to glucosamine metabolism through glycogenolysis.					
34043942	3	46	gly	glycosylation	293:305	arg1	brain					314:318	the brain	310:318	the brain	310:318	In this study, we found that N-linked protein glycosylation in the brain is metabolically channeled to glucosamine metabolism through glycogenolysis.					
34043942	4	47	theme	abundant	434:441	arg1	glucosamine					416:426	glucosamine	416:426	glucosamine	416:426	We discovered that glucosamine is an abundant constituent of brain glycogen, which functions as a glucosamine reservoir for multiple glycoconjugates.					
34043942	4	47	theme	abundant	434:441	arg1	reservoir					507:515	a glucosamine reservoir	493:515	a glucosamine reservoir for multiple glycoconjugates	493:544	We discovered that glucosamine is an abundant constituent of brain glycogen, which functions as a glucosamine reservoir for multiple glycoconjugates.					
34043942	4	47	theme	abundant	434:441	arg1	constituent					443:453	an abundant constituent	431:453	an abundant constituent	431:453	We discovered that glucosamine is an abundant constituent of brain glycogen, which functions as a glucosamine reservoir for multiple glycoconjugates.					
34043942	7	48	theme	fundamental	1040:1050	arg1	roles					1063:1067	fundamental biological roles	1040:1067	fundamental biological roles of brain glycogen in protein glycosylation	1040:1110	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	1	49	theme	many	130:133	arg1	diseases					150:157	many nervous system diseases	130:157	many nervous system diseases	130:157	Glycosylation defects are a hallmark of many nervous system diseases.					
34043942	6	50	theme	diseases	850:857	arg1	models					823:828	two mouse models	813:828	two mouse models of glycogen storage diseases	813:857	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	6	51	link	N-linked	984:991	arg1	glycosylation					1001:1013	N-linked protein glycosylation	984:1013	N-linked protein glycosylation	984:1013	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	7	52	theme	biological	1052:1061	arg1	roles					1063:1067	fundamental biological roles	1040:1067	fundamental biological roles of brain glycogen in protein glycosylation	1040:1110	These findings revealed fundamental biological roles of brain glycogen in protein glycosylation with direct relevance to multiple human diseases of the central nervous system.					
34043942	1	53	theme	nervous	135:141	arg1	diseases					150:157	many nervous system diseases	130:157	many nervous system diseases	130:157	Glycosylation defects are a hallmark of many nervous system diseases.					
34043942	6	54	from	decreases	929:937	arg1	pools					947:951	free pools	942:951	free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation	942:1013	Using two mouse models of glycogen storage diseases, we showed that disruption of brain glycogen metabolism causes global decreases in free pools of UDP-N-acetylglucosamine and N-linked protein glycosylation.					
34043942	4	55	theme	glucosamine	495:505	arg1	reservoir					507:515	a glucosamine reservoir	493:515	a glucosamine reservoir for multiple glycoconjugates	493:544	We discovered that glucosamine is an abundant constituent of brain glycogen, which functions as a glucosamine reservoir for multiple glycoconjugates.					
34043942	4	55	theme	glucosamine	495:505	arg1	constituent					443:453	an abundant constituent	431:453	an abundant constituent	431:453	We discovered that glucosamine is an abundant constituent of brain glycogen, which functions as a glucosamine reservoir for multiple glycoconjugates.					
34043942	1	56	theme	system	143:148	arg1	diseases					150:157	many nervous system diseases	130:157	many nervous system diseases	130:157	Glycosylation defects are a hallmark of many nervous system diseases.					
34102345	6	0	theme	further	896:902	arg1	extract					904:910	HMOs, further extract	890:910	extract	904:910	SIGNIFICANCE: We described a new method, which can successfully remove HMOs, further extract and purify the N-glycans on glycoproteins from pooled human milk for MALDI-TOF MS analysis by applying acetone precipitation and FASP together.					
34102345	5	1	theme	infant	782:787	arg1	formula					789:795	infant formula	782:795	infant formula in the food industry	782:816	Furthermore, we also compared both N-glycosylation and glycoproteins between human and bovine milk, which may provide new ideas for the composition adjustment of infant formula in the food industry.					
34102345	7	2	theme	N-glycosylation	1164:1178	arg1	differences					1180:1190	the N-glycosylation differences	1160:1190	the N-glycosylation differences between pooled human and bovine milk	1160:1227	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	4	3	theme	sample	539:544	arg1	FASP					566:569	FASP	566:569	FASP	566:569	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	4	3	theme	sample	539:544	arg1	method					558:563	the filter aided sample preparation method	522:563	the filter aided sample preparation method (FASP)	522:570	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	6	4	theme	MS	991:992	arg1	analysis					994:1001	MALDI-TOF MS analysis	981:1001	MALDI-TOF MS analysis	981:1001	SIGNIFICANCE: We described a new method, which can successfully remove HMOs, further extract and purify the N-glycans on glycoproteins from pooled human milk for MALDI-TOF MS analysis by applying acetone precipitation and FASP together.					
34102345	6	5	theme	pooled	959:964	arg1	milk					972:975	pooled human milk	959:975	pooled human milk for MALDI-TOF MS analysis	959:1001	SIGNIFICANCE: We described a new method, which can successfully remove HMOs, further extract and purify the N-glycans on glycoproteins from pooled human milk for MALDI-TOF MS analysis by applying acetone precipitation and FASP together.					
34102345	4	6	theme	aided	533:537	arg1	FASP					566:569	FASP	566:569	FASP	566:569	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	4	6	theme	aided	533:537	arg1	method					558:563	the filter aided sample preparation method	522:563	the filter aided sample preparation method (FASP)	522:570	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	7	7	theme	pooled	1128:1133	arg1	milk					1142:1145	pooled bovine milk	1128:1145	pooled bovine milk	1128:1145	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	6	8	theme	new	848:850	arg1	method					852:857	a new method	846:857	a new method	846:857	SIGNIFICANCE: We described a new method, which can successfully remove HMOs, further extract and purify the N-glycans on glycoproteins from pooled human milk for MALDI-TOF MS analysis by applying acetone precipitation and FASP together.					
34102345	7	9	theme	glycoproteins	1302:1314	arg1	pattern					1291:1297	N-glycan pattern	1282:1297	N-glycan pattern of glycoproteins	1282:1314	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	5	10	from	industry	809:816	arg1	adjustment					768:777	the composition adjustment	752:777	the composition adjustment of infant formula in the food industry	752:816	Furthermore, we also compared both N-glycosylation and glycoproteins between human and bovine milk, which may provide new ideas for the composition adjustment of infant formula in the food industry.					
34102345	3	11	theme	human	335:339	arg1	milk					341:344	human milk	335:344	human milk	335:344	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	5	12	from	adjustment	768:777	arg1	industry					809:816	the food industry	800:816	the food industry	800:816	Furthermore, we also compared both N-glycosylation and glycoproteins between human and bovine milk, which may provide new ideas for the composition adjustment of infant formula in the food industry.					
34102345	0	13	gly	glycoprotein	56:67	arg1	glycoprotein					56:67	milk glycoprotein	51:67	milk glycoprotein	51:67	A new method for purifying N-Glycans released from milk glycoprotein.					
34102345	4	14	theme	preparation	546:556	arg1	FASP					566:569	FASP	566:569	FASP	566:569	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	4	14	theme	preparation	546:556	arg1	method					558:563	the filter aided sample preparation method	522:563	the filter aided sample preparation method (FASP)	522:570	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	3	15	theme	abundant	313:320	arg1	component					322:330	the third most abundant component	298:330	the third most abundant component in human milk	298:344	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	7	16	theme	bovine	1217:1222	arg1	milk					1224:1227	pooled human and bovine milk	1200:1227	milk	1224:1227	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	5	17	theme	formula	789:795	arg1	adjustment					768:777	the composition adjustment	752:777	the composition adjustment of infant formula in the food industry	752:816	Furthermore, we also compared both N-glycosylation and glycoproteins between human and bovine milk, which may provide new ideas for the composition adjustment of infant formula in the food industry.					
34102345	7	18	theme	lectin	1356:1361	arg1	blotting					1363:1370	lectin blotting	1356:1370	lectin blotting	1356:1370	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	5	19	theme	food	804:807	arg1	industry					809:816	the food industry	800:816	the food industry	800:816	Furthermore, we also compared both N-glycosylation and glycoproteins between human and bovine milk, which may provide new ideas for the composition adjustment of infant formula in the food industry.					
34102345	1	20	theme	Human	70:74	arg1	source					94:99	the first source	84:99	the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring	84:193	Human milk is the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring.					
34102345	1	20	theme	Human	70:74	arg1	milk					76:79	Human milk	70:79	Human milk	70:79	Human milk is the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring.					
34102345	0	21	theme	new	2:4	arg1	method					6:11	A new method	0:11	A new method for purifying N-Glycans	0:35	A new method for purifying N-Glycans released from milk glycoprotein.					
34102345	7	22	theme	significant	1383:1393	arg1	differences					1395:1405	significant differences	1383:1405	significant differences in types and abundance of glycoproteins between the two sourced milk	1383:1474	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	6	23	theme	acetone	1015:1021	arg1	precipitation					1023:1035	acetone precipitation	1015:1035	acetone precipitation	1015:1035	SIGNIFICANCE: We described a new method, which can successfully remove HMOs, further extract and purify the N-glycans on glycoproteins from pooled human milk for MALDI-TOF MS analysis by applying acetone precipitation and FASP together.					
34102345	3	24	from	glycoproteins	438:450	arg1	milk					461:464	human milk	455:464	human milk	455:464	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	3	25	theme	free	353:356	arg1	HMOs					376:379	HMOs	376:379	HMOs	376:379	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	3	25	theme	free	353:356	arg1	oligosaccharides					358:373	human free oligosaccharides	347:373	human free oligosaccharides (HMOs)	347:380	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	6	26	from	N-glycans	927:935	arg1	glycoproteins					940:952	glycoproteins	940:952	glycoproteins	940:952	SIGNIFICANCE: We described a new method, which can successfully remove HMOs, further extract and purify the N-glycans on glycoproteins from pooled human milk for MALDI-TOF MS analysis by applying acetone precipitation and FASP together.					
34102345	2	27	dep	good	241:244	arg1	substitutes					246:256	substitutes	246:256	substitutes	246:256	Modern bovine-milk-based infant formulas are good substitutes when mother's milk is not available.					
34102345	7	28	from	differences	1395:1405	arg1	abundance					1420:1428	abundance	1420:1428	abundance	1420:1428	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	7	28	from	differences	1395:1405	arg1	types					1410:1414	types	1410:1414	types	1410:1414	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	5	29	theme	composition	756:766	arg1	adjustment					768:777	the composition adjustment	752:777	the composition adjustment of infant formula in the food industry	752:816	Furthermore, we also compared both N-glycosylation and glycoproteins between human and bovine milk, which may provide new ideas for the composition adjustment of infant formula in the food industry.					
34102345	3	30	gly	glycoproteins	438:450	arg1	glycoproteins					438:450	the glycoproteins	434:450	the glycoproteins in human milk	434:464	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	3	31	theme	N-glycans	421:429	arg1	analysis					403:410	the analysis	399:410	the analysis of total N-glycans	399:429	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	3	32	from	interference	386:397	arg1	glycoproteins					438:450	the glycoproteins	434:450	the glycoproteins in human milk	434:464	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	4	33	theme	filter	526:531	arg1	FASP					566:569	FASP	566:569	FASP	566:569	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	4	33	theme	filter	526:531	arg1	method					558:563	the filter aided sample preparation method	522:563	the filter aided sample preparation method (FASP)	522:570	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	7	34	from	proteins	1116:1123	arg1	N-glycans					1096:1104	the N-glycans	1092:1104	the N-glycans from whey proteins in pooled bovine milk	1092:1145	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	7	34	from	proteins	1116:1123	arg1	milk					1142:1145	pooled bovine milk	1128:1145	pooled bovine milk	1128:1145	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	1	35	theme	first	88:92	arg1	source					94:99	the first source	84:99	the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring	84:193	Human milk is the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring.					
34102345	1	35	theme	first	88:92	arg1	milk					76:79	Human milk	70:79	Human milk	70:79	Human milk is the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring.					
34102345	1	36	theme	components	171:180	arg1	array					145:149	an array	142:149	an array of unique bioactive components to offspring	142:193	Human milk is the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring.					
34102345	3	37	from	component	322:330	arg1	milk					341:344	human milk	335:344	human milk	335:344	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	2	38	theme	infant	221:226	arg1	formulas					228:235	Modern bovine-milk-based infant formulas	196:235	Modern bovine-milk-based infant formulas	196:235	Modern bovine-milk-based infant formulas are good substitutes when mother's milk is not available.					
34102345	1	39	theme	unique	154:159	arg1	components					171:180	unique bioactive components	154:180	unique bioactive components	154:180	Human milk is the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring.					
34102345	0	40	theme	purifying	17:25	arg1	N-Glycans					27:35	purifying N-Glycans	17:35	purifying N-Glycans	17:35	A new method for purifying N-Glycans released from milk glycoprotein.					
34102345	6	41	theme	HMOs	890:893	arg1	extract					904:910	HMOs, further extract	890:910	extract	904:910	SIGNIFICANCE: We described a new method, which can successfully remove HMOs, further extract and purify the N-glycans on glycoproteins from pooled human milk for MALDI-TOF MS analysis by applying acetone precipitation and FASP together.					
34102345	5	42	theme	new	738:740	arg1	ideas					742:746	new ideas	738:746	new ideas	738:746	Furthermore, we also compared both N-glycosylation and glycoproteins between human and bovine milk, which may provide new ideas for the composition adjustment of infant formula in the food industry.					
34102345	7	43	theme	sourced	1463:1469	arg1	milk					1471:1474	the two sourced milk	1455:1474	the two sourced milk	1455:1474	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	2	44	theme	bovine-milk-based	203:219	arg1	formulas					228:235	Modern bovine-milk-based infant formulas	196:235	Modern bovine-milk-based infant formulas	196:235	Modern bovine-milk-based infant formulas are good substitutes when mother's milk is not available.					
34102345	6	45	theme	MALDI-TOF	981:989	arg1	analysis					994:1001	MALDI-TOF MS analysis	981:1001	MALDI-TOF MS analysis	981:1001	SIGNIFICANCE: We described a new method, which can successfully remove HMOs, further extract and purify the N-glycans on glycoproteins from pooled human milk for MALDI-TOF MS analysis by applying acetone precipitation and FASP together.					
34102345	3	46	theme	total	415:419	arg1	N-glycans					421:429	total N-glycans	415:429	total N-glycans	415:429	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	7	47	theme	new	1071:1073	arg1	method					1075:1080	the new method	1067:1080	the new method to purify the N-glycans from whey proteins in pooled bovine milk	1067:1145	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	2	48	theme	Modern	196:201	arg1	formulas					228:235	Modern bovine-milk-based infant formulas	196:235	Modern bovine-milk-based infant formulas	196:235	Modern bovine-milk-based infant formulas are good substitutes when mother's milk is not available.					
34102345	7	49	gly	glycoproteins	1433:1445	arg1	glycoproteins					1433:1445	glycoproteins	1433:1445	glycoproteins	1433:1445	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	7	50	gly	glycoproteins	1302:1314	arg1	glycoproteins					1302:1314	glycoproteins	1302:1314	glycoproteins	1302:1314	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	4	51	theme	precipitation	495:507	arg1	protein					509:515	acetone precipitation protein	487:515	acetone precipitation protein with the filter aided sample preparation method (FASP)	487:570	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	7	52	theme	human	1342:1346	arg1	milk					1348:1351	pooled bovine and human milk	1324:1351	milk	1348:1351	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	3	53	theme	human	455:459	arg1	milk					461:464	human milk	455:464	human milk	455:464	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	4	54	theme	acetone	487:493	arg1	protein					509:515	acetone precipitation protein	487:515	acetone precipitation protein with the filter aided sample preparation method (FASP)	487:570	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	7	55	theme	pattern	1291:1297	arg1	difference					1268:1277	the difference	1264:1277	the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk	1264:1351	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	5	56	from	formula	789:795	arg1	industry					809:816	the food industry	800:816	the food industry	800:816	Furthermore, we also compared both N-glycosylation and glycoproteins between human and bovine milk, which may provide new ideas for the composition adjustment of infant formula in the food industry.					
34102345	1	57	theme	nutrition	104:112	arg1	source					94:99	the first source	84:99	the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring	84:193	Human milk is the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring.					
34102345	1	57	theme	nutrition	104:112	arg1	milk					76:79	Human milk	70:79	Human milk	70:79	Human milk is the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring.					
34102345	6	58	theme	human	966:970	arg1	milk					972:975	pooled human milk	959:975	pooled human milk for MALDI-TOF MS analysis	959:1001	SIGNIFICANCE: We described a new method, which can successfully remove HMOs, further extract and purify the N-glycans on glycoproteins from pooled human milk for MALDI-TOF MS analysis by applying acetone precipitation and FASP together.					
34102345	4	59	with	protein	509:515	arg1	FASP					566:569	FASP	566:569	FASP	566:569	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	4	59	with	protein	509:515	arg1	method					558:563	the filter aided sample preparation method	522:563	the filter aided sample preparation method (FASP)	522:570	Herein, we combined acetone precipitation protein with the filter aided sample preparation method (FASP) to thoroughly remove HMOs and purify N-glycans.					
34102345	7	60	theme	glycoproteins	1433:1445	arg1	abundance					1420:1428	abundance	1420:1428	abundance	1420:1428	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	7	60	theme	glycoproteins	1433:1445	arg1	types					1410:1414	types	1410:1414	types	1410:1414	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	5	61	gly	glycoproteins	675:687	arg1	glycoproteins					675:687	glycoproteins	675:687	glycoproteins	675:687	Furthermore, we also compared both N-glycosylation and glycoproteins between human and bovine milk, which may provide new ideas for the composition adjustment of infant formula in the food industry.					
34102345	3	62	theme	third	302:306	arg1	component					322:330	the third most abundant component	298:330	the third most abundant component in human milk	298:344	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	1	63	theme	bioactive	161:169	arg1	components					171:180	unique bioactive components	154:180	unique bioactive components	154:180	Human milk is the first source of nutrition for infants, which delivers an array of unique bioactive components to offspring.					
34102345	5	64	dep	human	697:701	arg1	milk					714:717	milk	714:717	milk	714:717	Furthermore, we also compared both N-glycosylation and glycoproteins between human and bovine milk, which may provide new ideas for the composition adjustment of infant formula in the food industry.					
34102345	0	65	theme	milk	51:54	arg1	glycoprotein					56:67	milk glycoprotein	51:67	milk glycoprotein	51:67	A new method for purifying N-Glycans released from milk glycoprotein.					
34102345	7	66	theme	N-glycan	1282:1289	arg1	pattern					1291:1297	N-glycan pattern	1282:1297	N-glycan pattern of glycoproteins	1282:1314	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	6	67	gly	glycoproteins	940:952	arg1	glycoproteins					940:952	glycoproteins	940:952	glycoproteins	940:952	SIGNIFICANCE: We described a new method, which can successfully remove HMOs, further extract and purify the N-glycans on glycoproteins from pooled human milk for MALDI-TOF MS analysis by applying acetone precipitation and FASP together.					
34102345	3	68	theme	human	347:351	arg1	HMOs					376:379	HMOs	376:379	HMOs	376:379	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	3	68	theme	human	347:351	arg1	oligosaccharides					358:373	human free oligosaccharides	347:373	human free oligosaccharides (HMOs)	347:380	As the third most abundant component in human milk, human free oligosaccharides (HMOs) may interference the analysis of total N-glycans on the glycoproteins in human milk.					
34102345	7	69	theme	whey	1111:1114	arg1	proteins					1116:1123	whey proteins	1111:1123	whey proteins in pooled bovine milk	1111:1145	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
34102345	7	70	theme	bovine	1135:1140	arg1	milk					1142:1145	pooled bovine milk	1128:1145	pooled bovine milk	1128:1145	We applied the new method to purify the N-glycans from whey proteins in pooled bovine milk and compared the N-glycosylation differences between pooled human and bovine milk by MALDI-TOF MS. We first reported the difference of N-glycan pattern of glycoproteins between pooled bovine and human milk by lectin blotting, and found significant differences in types and abundance of glycoproteins between the two sourced milk.					
33334107	2	0	theme	N-acetyl	511:518	arg1	moieties					534:541	N-acetyl and N-sulfate moieties	511:541	N-acetyl and N-sulfate moieties	511:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	2	1	theme	modular	353:359	arg1	methodology					371:381	a modular synthetic methodology	351:381	a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties	351:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	1	2	theme	proteins	252:259	arg1	multitude					239:247	a multitude	237:247	a multitude of proteins	237:259	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	5	3	theme	levulinic	954:962	arg1	ester					970:974	levulinic (Lev) ester	954:974	levulinic (Lev) ester	954:974	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	3	theme	levulinic	954:962	arg1	groups					947:952	the orthogonal hydroxyl protecting groups	912:952	the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc)	912:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	4	theme	hydroxyl	927:934	arg1	ester					970:974	levulinic (Lev) ester	954:974	levulinic (Lev) ester	954:974	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	4	theme	hydroxyl	927:934	arg1	carbonate					1059:1067	9-fluorenylmethyl carbonate	1041:1067	9-fluorenylmethyl carbonate (Fmoc)	1041:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	4	theme	hydroxyl	927:934	arg1	allyloxycarbonate					1010:1026	allyloxycarbonate	1010:1026	allyloxycarbonate (Alloc)	1010:1034	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	4	theme	hydroxyl	927:934	arg1	groups					947:952	the orthogonal hydroxyl protecting groups	912:952	the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc)	912:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	4	theme	hydroxyl	927:934	arg1	ether					1003:1007	thexyldimethylsilyl (TDS) ether	977:1007	thexyldimethylsilyl (TDS) ether	977:1007	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	5	theme	protecting	936:945	arg1	ester					970:974	levulinic (Lev) ester	954:974	levulinic (Lev) ester	954:974	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	5	theme	protecting	936:945	arg1	carbonate					1059:1067	9-fluorenylmethyl carbonate	1041:1067	9-fluorenylmethyl carbonate (Fmoc)	1041:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	5	theme	protecting	936:945	arg1	allyloxycarbonate					1010:1026	allyloxycarbonate	1010:1026	allyloxycarbonate (Alloc)	1010:1034	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	5	theme	protecting	936:945	arg1	groups					947:952	the orthogonal hydroxyl protecting groups	912:952	the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc)	912:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	5	theme	protecting	936:945	arg1	ether					1003:1007	thexyldimethylsilyl (TDS) ether	977:1007	thexyldimethylsilyl (TDS) ether	977:1007	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	3	6	theme	α-glycosides	712:723	arg1	installation					696:707	the selective installation	682:707	the selective installation of α-glycosides	682:723	It is based on the use of glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides.					
33334107	2	7	contain	have	451:454	arg2	residues					468:475	glucosamine residues	456:475	glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties	456:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	2	7	contain	have	451:454	arg1	oligosaccharides					429:444	heparan sulfate oligosaccharides	413:444	heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties	413:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	5	8	theme	orthogonal	916:925	arg1	ester					970:974	levulinic (Lev) ester	954:974	levulinic (Lev) ester	954:974	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	8	theme	orthogonal	916:925	arg1	carbonate					1059:1067	9-fluorenylmethyl carbonate	1041:1067	9-fluorenylmethyl carbonate (Fmoc)	1041:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	8	theme	orthogonal	916:925	arg1	allyloxycarbonate					1010:1026	allyloxycarbonate	1010:1026	allyloxycarbonate (Alloc)	1010:1034	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	8	theme	orthogonal	916:925	arg1	groups					947:952	the orthogonal hydroxyl protecting groups	912:952	the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc)	912:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	8	theme	orthogonal	916:925	arg1	ether					1003:1007	thexyldimethylsilyl (TDS) ether	977:1007	thexyldimethylsilyl (TDS) ether	977:1007	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	9	theme	thexyldimethylsilyl	977:995	arg1	groups					947:952	the orthogonal hydroxyl protecting groups	912:952	the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc)	912:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	9	theme	thexyldimethylsilyl	977:995	arg1	ether					1003:1007	thexyldimethylsilyl (TDS) ether	977:1007	thexyldimethylsilyl (TDS) ether	977:1007	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	3	10	mod	modified	595:602	arg3	moiety					660:665	an azido- or trifluoromethylphenyl-methanimine moiety	613:665	an azido- or trifluoromethylphenyl-methanimine moiety	613:665	It is based on the use of glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides.					
33334107	3	10	mod	modified	595:602	arg1	donors					579:584	glycosyl donors	570:584	glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides	570:723	It is based on the use of glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides.					
33334107	5	11	theme	O-sulfation	1099:1109	arg1	patterns					1087:1094	different patterns	1077:1094	different patterns of O-sulfation	1077:1109	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	12	theme	TDS	998:1000	arg1	groups					947:952	the orthogonal hydroxyl protecting groups	912:952	the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc)	912:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	12	theme	TDS	998:1000	arg1	ether					1003:1007	thexyldimethylsilyl (TDS) ether	977:1007	thexyldimethylsilyl (TDS) ether	977:1007	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	4	13	theme	a	785:785	arg1	reduction					787:795	a reduction	785:795	a reduction	785:795	The amino protecting groups can be selectively unmasked by a reduction or acid treatment, allowing the installation of N-acetyl and N-sulfate moieties, respectively.					
33334107	7	14	theme	proteins	1418:1425	arg1	selectivities					1382:1394	the binding selectivities	1370:1394	the binding selectivities of several HS-binding proteins	1370:1425	These compounds, together with a number of previously prepared HS oligosaccharides, were printed as a glycan microarray to examine the binding selectivities of several HS-binding proteins.					
33334107	4	15	theme	protecting	736:745	arg1	groups					747:752	The amino protecting groups	726:752	The amino protecting groups	726:752	The amino protecting groups can be selectively unmasked by a reduction or acid treatment, allowing the installation of N-acetyl and N-sulfate moieties, respectively.					
33334107	4	16	theme	moieties	868:875	arg1	installation					829:840	the installation	825:840	the installation of N-acetyl and N-sulfate moieties	825:875	The amino protecting groups can be selectively unmasked by a reduction or acid treatment, allowing the installation of N-acetyl and N-sulfate moieties, respectively.					
33334107	4	17	theme	acid	800:803	arg1	treatment					805:813	acid treatment	800:813	acid treatment	800:813	The amino protecting groups can be selectively unmasked by a reduction or acid treatment, allowing the installation of N-acetyl and N-sulfate moieties, respectively.					
33334107	2	18	theme	moieties	534:541	arg1	patterns					499:506	different patterns	489:506	different patterns of N-acetyl and N-sulfate moieties	489:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	4	19	theme	N-sulfate	858:866	arg1	moieties					868:875	N-acetyl and N-sulfate moieties	845:875	N-acetyl and N-sulfate moieties	845:875	The amino protecting groups can be selectively unmasked by a reduction or acid treatment, allowing the installation of N-acetyl and N-sulfate moieties, respectively.					
33334107	0	20	theme	Modular	0:6	arg1	Synthesis					8:16	Modular Synthesis	0:16	Modular Synthesis of Heparan Sulfate Oligosaccharides	0:52	Modular Synthesis of Heparan Sulfate Oligosaccharides Having N-Acetyl and N-Sulfate Moieties.					
33334107	7	21	theme	prepared	1293:1300	arg1	oligosaccharides					1305:1320	previously prepared HS oligosaccharides	1282:1320	previously prepared HS oligosaccharides	1282:1320	These compounds, together with a number of previously prepared HS oligosaccharides, were printed as a glycan microarray to examine the binding selectivities of several HS-binding proteins.					
33334107	2	22	theme	N-sulfate	524:532	arg1	moieties					534:541	N-acetyl and N-sulfate moieties	511:541	N-acetyl and N-sulfate moieties	511:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	1	23	theme	Heparan	94:100	arg1	polysaccharides					145:159	structurally diverse sulfated polysaccharides	115:159	structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes	115:331	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	1	23	theme	Heparan	94:100	arg1	sulfates					102:109	Heparan sulfates	94:109	Heparan sulfates	94:109	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	1	24	theme	animal	195:200	arg1	cells					202:206	all animal cells	191:206	all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes	191:331	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	2	25	theme	glucosamine	456:466	arg1	residues					468:475	glucosamine residues	456:475	glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties	456:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	1	26	theme	cells	202:206	arg1	surface					180:186	the surface	176:186	the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes	176:331	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	0	27	theme	Heparan	21:27	arg1	Oligosaccharides					37:52	Heparan Sulfate Oligosaccharides	21:52	Heparan Sulfate Oligosaccharides	21:52	Modular Synthesis of Heparan Sulfate Oligosaccharides Having N-Acetyl and N-Sulfate Moieties.					
33334107	1	28	theme	wide	283:286	arg1	range					288:292	a wide range	281:292	a wide range of physiological and disease processes	281:331	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	3	29	theme	donors	579:584	arg1	use					563:565	the use	559:565	the use of glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides	559:723	It is based on the use of glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides.					
33334107	7	30	theme	several	1399:1405	arg1	proteins					1418:1425	several HS-binding proteins	1399:1425	several HS-binding proteins	1399:1425	These compounds, together with a number of previously prepared HS oligosaccharides, were printed as a glycan microarray to examine the binding selectivities of several HS-binding proteins.					
33334107	2	31	theme	oligosaccharides	429:444	arg1	libraries					400:408	libraries	400:408	libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties	400:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	5	32	dep	groups	947:952	arg1	carbonate					1059:1067	9-fluorenylmethyl carbonate	1041:1067	9-fluorenylmethyl carbonate (Fmoc)	1041:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	32	dep	groups	947:952	arg1	Alloc					1029:1033	Alloc	1029:1033	Alloc	1029:1033	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	32	dep	groups	947:952	arg1	Fmoc					1070:1073	Fmoc	1070:1073	Fmoc	1070:1073	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	32	dep	groups	947:952	arg1	ether					1003:1007	thexyldimethylsilyl (TDS) ether	977:1007	thexyldimethylsilyl (TDS) ether	977:1007	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	32	dep	groups	947:952	arg1	ester					970:974	levulinic (Lev) ester	954:974	levulinic (Lev) ester	954:974	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	32	dep	groups	947:952	arg1	allyloxycarbonate					1010:1026	allyloxycarbonate	1010:1026	allyloxycarbonate (Alloc)	1010:1034	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	32	dep	groups	947:952	arg1	groups					947:952	the orthogonal hydroxyl protecting groups	912:952	the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc)	912:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	3	33	theme	glycosyl	570:577	arg1	donors					579:584	glycosyl donors	570:584	glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides	570:723	It is based on the use of glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides.					
33334107	2	34	theme	sulfate	421:427	arg1	oligosaccharides					429:444	heparan sulfate oligosaccharides	413:444	heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties	413:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	0	35	theme	Oligosaccharides	37:52	arg1	Synthesis					8:16	Modular Synthesis	0:16	Modular Synthesis of Heparan Sulfate Oligosaccharides	0:52	Modular Synthesis of Heparan Sulfate Oligosaccharides Having N-Acetyl and N-Sulfate Moieties.					
33334107	4	36	theme	amino	730:734	arg1	groups					747:752	The amino protecting groups	726:752	The amino protecting groups	726:752	The amino protecting groups can be selectively unmasked by a reduction or acid treatment, allowing the installation of N-acetyl and N-sulfate moieties, respectively.					
33334107	5	37	theme	different	1077:1085	arg1	patterns					1087:1094	different patterns	1077:1094	different patterns of O-sulfation	1077:1109	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	2	38	theme	heparan	413:419	arg1	oligosaccharides					429:444	heparan sulfate oligosaccharides	413:444	heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties	413:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	0	39	theme	Sulfate	29:35	arg1	Oligosaccharides					37:52	Heparan Sulfate Oligosaccharides	21:52	Heparan Sulfate Oligosaccharides	21:52	Modular Synthesis of Heparan Sulfate Oligosaccharides Having N-Acetyl and N-Sulfate Moieties.					
33334107	1	40	theme	physiological	297:309	arg1	range					288:292	a wide range	281:292	a wide range of physiological and disease processes	281:331	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	2	41	theme	different	489:497	arg1	patterns					499:506	different patterns	489:506	different patterns of N-acetyl and N-sulfate moieties	489:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
33334107	3	42	theme	trifluoromethylphenyl-methanimine	626:658	arg1	moiety					660:665	an azido- or trifluoromethylphenyl-methanimine moiety	613:665	an azido- or trifluoromethylphenyl-methanimine moiety	613:665	It is based on the use of glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides.					
33334107	7	43	theme	binding	1374:1380	arg1	selectivities					1382:1394	the binding selectivities	1370:1394	the binding selectivities of several HS-binding proteins	1370:1425	These compounds, together with a number of previously prepared HS oligosaccharides, were printed as a glycan microarray to examine the binding selectivities of several HS-binding proteins.					
33334107	7	44	theme	HS	1302:1303	arg1	oligosaccharides					1305:1320	previously prepared HS oligosaccharides	1282:1320	previously prepared HS oligosaccharides	1282:1320	These compounds, together with a number of previously prepared HS oligosaccharides, were printed as a glycan microarray to examine the binding selectivities of several HS-binding proteins.					
33334107	0	45	theme	N-Acetyl	61:68	arg1	Moieties					84:91	N-Acetyl and N-Sulfate Moieties	61:91	N-Acetyl and N-Sulfate Moieties	61:91	Modular Synthesis of Heparan Sulfate Oligosaccharides Having N-Acetyl and N-Sulfate Moieties.					
33334107	5	46	with	combination	895:905	arg1	ester					970:974	levulinic (Lev) ester	954:974	levulinic (Lev) ester	954:974	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	46	with	combination	895:905	arg1	carbonate					1059:1067	9-fluorenylmethyl carbonate	1041:1067	9-fluorenylmethyl carbonate (Fmoc)	1041:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	46	with	combination	895:905	arg1	allyloxycarbonate					1010:1026	allyloxycarbonate	1010:1026	allyloxycarbonate (Alloc)	1010:1034	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	46	with	combination	895:905	arg1	groups					947:952	the orthogonal hydroxyl protecting groups	912:952	the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc)	912:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	46	with	combination	895:905	arg1	ether					1003:1007	thexyldimethylsilyl (TDS) ether	977:1007	thexyldimethylsilyl (TDS) ether	977:1007	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	6	47	theme	O-sulfation	1226:1236	arg1	pattern					1208:1214	the pattern	1204:1214	the pattern of N- and O-sulfation	1204:1236	The methodology was applied to prepare four hexasaccharides that differ in the pattern of N- and O-sulfation.					
33334107	1	48	theme	diverse	128:134	arg1	polysaccharides					145:159	structurally diverse sulfated polysaccharides	115:159	structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes	115:331	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	1	48	theme	diverse	128:134	arg1	sulfates					102:109	Heparan sulfates	94:109	Heparan sulfates	94:109	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	1	49	theme	disease	315:321	arg1	processes					323:331	disease processes	315:331	disease processes	315:331	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	3	50	theme	selective	686:694	arg1	installation					696:707	the selective installation	682:707	the selective installation of α-glycosides	682:723	It is based on the use of glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides.					
33334107	4	51	theme	N-acetyl	845:852	arg1	moieties					868:875	N-acetyl and N-sulfate moieties	845:875	N-acetyl and N-sulfate moieties	845:875	The amino protecting groups can be selectively unmasked by a reduction or acid treatment, allowing the installation of N-acetyl and N-sulfate moieties, respectively.					
33334107	1	52	theme	sulfated	136:143	arg1	polysaccharides					145:159	structurally diverse sulfated polysaccharides	115:159	structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes	115:331	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	1	52	theme	sulfated	136:143	arg1	sulfates					102:109	Heparan sulfates	94:109	Heparan sulfates	94:109	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	1	53	theme	processes	323:331	arg1	range					288:292	a wide range	281:292	a wide range of physiological and disease processes	281:331	Heparan sulfates are structurally diverse sulfated polysaccharides that reside at the surface of all animal cells where they can interact with a multitude of proteins, thereby modulating a wide range of physiological and disease processes.					
33334107	6	54	theme	N-	1219:1220	arg1	pattern					1208:1214	the pattern	1204:1214	the pattern of N- and O-sulfation	1204:1236	The methodology was applied to prepare four hexasaccharides that differ in the pattern of N- and O-sulfation.					
33334107	3	55	theme	azido-	616:621	arg1	moiety					660:665	an azido- or trifluoromethylphenyl-methanimine moiety	613:665	an azido- or trifluoromethylphenyl-methanimine moiety	613:665	It is based on the use of glycosyl donors that are modified at C2 by an azido- or trifluoromethylphenyl-methanimine moiety, which allowed the selective installation of α-glycosides.					
33334107	7	56	theme	HS-binding	1407:1416	arg1	proteins					1418:1425	several HS-binding proteins	1399:1425	several HS-binding proteins	1399:1425	These compounds, together with a number of previously prepared HS oligosaccharides, were printed as a glycan microarray to examine the binding selectivities of several HS-binding proteins.					
33334107	5	57	theme	Lev	965:967	arg1	ester					970:974	levulinic (Lev) ester	954:974	levulinic (Lev) ester	954:974	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	57	theme	Lev	965:967	arg1	groups					947:952	the orthogonal hydroxyl protecting groups	912:952	the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc)	912:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	58	theme	9-fluorenylmethyl	1041:1057	arg1	Fmoc					1070:1073	Fmoc	1070:1073	Fmoc	1070:1073	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	58	theme	9-fluorenylmethyl	1041:1057	arg1	carbonate					1059:1067	9-fluorenylmethyl carbonate	1041:1067	9-fluorenylmethyl carbonate (Fmoc)	1041:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	5	58	theme	9-fluorenylmethyl	1041:1057	arg1	groups					947:952	the orthogonal hydroxyl protecting groups	912:952	the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc)	912:1074	In combination with the orthogonal hydroxyl protecting groups levulinic (Lev) ester, thexyldimethylsilyl (TDS) ether, allyloxycarbonate (Alloc), and 9-fluorenylmethyl carbonate (Fmoc), different patterns of O-sulfation can be installed.					
33334107	0	59	theme	N-Sulfate	74:82	arg1	Moieties					84:91	N-Acetyl and N-Sulfate Moieties	61:91	N-Acetyl and N-Sulfate Moieties	61:91	Modular Synthesis of Heparan Sulfate Oligosaccharides Having N-Acetyl and N-Sulfate Moieties.					
33334107	7	60	theme	glycan	1341:1346	arg1	compounds					1245:1253	These compounds	1239:1253	These compounds	1239:1253	These compounds, together with a number of previously prepared HS oligosaccharides, were printed as a glycan microarray to examine the binding selectivities of several HS-binding proteins.					
33334107	7	60	theme	glycan	1341:1346	arg1	microarray					1348:1357	a glycan microarray	1339:1357	a glycan microarray to examine the binding selectivities of several HS-binding proteins	1339:1425	These compounds, together with a number of previously prepared HS oligosaccharides, were printed as a glycan microarray to examine the binding selectivities of several HS-binding proteins.					
33334107	7	61	theme	oligosaccharides	1305:1320	arg1	number					1272:1277	a number	1270:1277	a number of previously prepared HS oligosaccharides	1270:1320	These compounds, together with a number of previously prepared HS oligosaccharides, were printed as a glycan microarray to examine the binding selectivities of several HS-binding proteins.					
33334107	2	62	theme	synthetic	361:369	arg1	methodology					371:381	a modular synthetic methodology	351:381	a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties	351:541	We describe here a modular synthetic methodology that can provide libraries of heparan sulfate oligosaccharides that have glucosamine residues modified by different patterns of N-acetyl and N-sulfate moieties.					
32236230	10	0	theme	intact	1652:1657	arg1	characterization					1664:1679	intact mass characterization	1652:1679	intact mass characterization	1652:1679	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	10	1	gly	di-sialylation	1700:1713	arg1	Herceptin					1718:1726	Herceptin	1718:1726	Herceptin	1718:1726	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	6	2	theme	antibody	940:947	arg1	remodeling					949:958	antibody remodeling	940:958	antibody remodeling	940:958	To overcome the low proficiency of bacterial sialyltransferase in antibody remodeling, the Photobacterium sp.					
32236230	2	3	theme	IgG	309:311	arg1	antibodies					313:322	IgG antibodies	309:322	IgG antibodies	309:322	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	5	4	theme	bacterial	708:716	arg1	sialyltransferase					718:734	a bacterial sialyltransferase	706:734	a bacterial sialyltransferase to facilitate the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation	706:871	Herein, a one-pot three-enzyme protocol was developed by engineering a bacterial sialyltransferase to facilitate the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation.					
32236230	6	5	theme	sialyltransferase	919:935	arg1	proficiency					894:904	the low proficiency	886:904	the low proficiency of bacterial sialyltransferase in antibody remodeling	886:958	To overcome the low proficiency of bacterial sialyltransferase in antibody remodeling, the Photobacterium sp.					
32236230	7	6	theme	JT-ISH-224	984:993	arg1	α-2,6-sialyltransferase					995:1017	JT-ISH-224 α-2,6-sialyltransferase	984:1017	JT-ISH-224 α-2,6-sialyltransferase (Psp2,6ST)	984:1028	JT-ISH-224 α-2,6-sialyltransferase (Psp2,6ST) was genetically engineered by terminal truncation and site-directed mutagenesis based on its protein crystal structure.					
32236230	7	6	theme	JT-ISH-224	984:993	arg1	Psp2,6ST					1020:1027	Psp2,6ST	1020:1027	Psp2,6ST	1020:1027	JT-ISH-224 α-2,6-sialyltransferase (Psp2,6ST) was genetically engineered by terminal truncation and site-directed mutagenesis based on its protein crystal structure.					
32236230	8	7	theme	antibody	1370:1377	arg1	di-sialylation					1379:1392	antibody di-sialylation	1370:1392	antibody di-sialylation	1370:1392	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	8	8	theme	reaction	1169:1176	arg1	conditions					1178:1187	the optimized reaction conditions	1155:1187	the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants	1155:1251	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	1	9	theme	glycoproteins	213:225	arg1	efficacy					189:196	efficacy	189:196	efficacy	189:196	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	1	9	theme	glycoproteins	213:225	arg1	properties					174:183	properties	174:183	properties	174:183	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	7	10	theme	site-directed	1084:1096	arg1	mutagenesis					1098:1108	site-directed mutagenesis	1084:1108	site-directed mutagenesis	1084:1108	JT-ISH-224 α-2,6-sialyltransferase (Psp2,6ST) was genetically engineered by terminal truncation and site-directed mutagenesis based on its protein crystal structure.					
32236230	9	11	theme	substrate	1419:1427	arg1	promiscuity					1429:1439	the donor substrate promiscuity	1409:1439	the donor substrate promiscuity	1409:1439	Herceptin and the donor substrate promiscuity allow the introduction of bioorthogonal modifications of N-acetylneuraminic acid into antibodies for site-specific conjugation.					
32236230	2	12	with	N-glycan	261:268	arg1	acids					299:303	two α-2,6-linked sialic acids	275:303	two α-2,6-linked sialic acids	275:303	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	1	13	gly	glycosylation	143:155	arg1	glycoproteins					213:225	therapeutic glycoproteins	201:225	therapeutic glycoproteins	201:225	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	5	14	theme	antibodies	782:791	arg1	modification					754:765	the modification	750:765	the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation	750:871	Herein, a one-pot three-enzyme protocol was developed by engineering a bacterial sialyltransferase to facilitate the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation.					
32236230	1	15	theme	humanized	133:141	arg1	glycosylation					143:155	a humanized glycosylation	131:155	a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins	131:225	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	1	15	theme	humanized	133:141	arg1	α-2,6-sialylation					97:113	Terminal α-2,6-sialylation	88:113	Terminal α-2,6-sialylation of N-glycans	88:126	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	12	16	theme	conjugation	2054:2064	arg1	glycoengineering					1984:1999	the enzymatic glycoengineering	1970:1999	the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies	1970:2078	It provides new insights into engineering bacterial sialyltransferase for adaptation to the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies.					
32236230	5	17	theme	N-acetylneuraminic	798:815	arg1	acid					817:820	N-acetylneuraminic acid	798:820	N-acetylneuraminic acid	798:820	Herein, a one-pot three-enzyme protocol was developed by engineering a bacterial sialyltransferase to facilitate the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation.					
32236230	9	18	theme	bioorthogonal	1467:1479	arg1	modifications					1481:1493	bioorthogonal modifications	1467:1493	bioorthogonal modifications of N-acetylneuraminic acid	1467:1520	Herceptin and the donor substrate promiscuity allow the introduction of bioorthogonal modifications of N-acetylneuraminic acid into antibodies for site-specific conjugation.					
32236230	2	19	theme	sialic	292:297	arg1	acids					299:303	two α-2,6-linked sialic acids	275:303	two α-2,6-linked sialic acids	275:303	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	12	20	theme	antibodies	2069:2078	arg1	conjugation					2054:2064	the glycosite-specific conjugation	2031:2064	the glycosite-specific conjugation of antibodies	2031:2078	It provides new insights into engineering bacterial sialyltransferase for adaptation to the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies.					
32236230	12	20	theme	antibodies	2069:2078	arg1	antibodies					2016:2025	therapeutic antibodies	2004:2025	therapeutic antibodies	2004:2025	It provides new insights into engineering bacterial sialyltransferase for adaptation to the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies.					
32236230	0	21	theme	therapeutic	66:76	arg1	antibody					78:85	a therapeutic antibody	64:85	a therapeutic antibody	64:85	Engineering a bacterial sialyltransferase for di-sialylation of a therapeutic antibody.					
32236230	12	22	theme	antibodies	2016:2025	arg1	glycoengineering					1984:1999	the enzymatic glycoengineering	1970:1999	the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies	1970:2078	It provides new insights into engineering bacterial sialyltransferase for adaptation to the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies.					
32236230	10	23	theme	Herceptin	1718:1726	arg1	di-sialylation					1700:1713	the high di-sialylation	1691:1713	the high di-sialylation of Herceptin	1691:1726	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	11	24	theme	versatile	1802:1810	arg1	approach					1822:1829	a versatile enzymatic approach	1800:1829	a versatile enzymatic approach for producing highly di-sialylated IgG antibodies	1800:1879	This study established a versatile enzymatic approach for producing highly di-sialylated IgG antibodies.					
32236230	2	25	link	α-2,6-linked	279:290	arg1	acids					299:303	two α-2,6-linked sialic acids	275:303	two α-2,6-linked sialic acids	275:303	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	9	26	theme	acid	1517:1520	arg1	modifications					1481:1493	bioorthogonal modifications	1467:1493	bioorthogonal modifications of N-acetylneuraminic acid	1467:1520	Herceptin and the donor substrate promiscuity allow the introduction of bioorthogonal modifications of N-acetylneuraminic acid into antibodies for site-specific conjugation.					
32236230	8	27	theme	activity-based	1199:1212	arg1	screening					1214:1222	activity-based screening	1199:1222	activity-based screening of various Psp2,6ST variants	1199:1251	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	7	28	theme	crystal	1131:1137	arg1	structure					1139:1147	its protein crystal structure	1119:1147	its protein crystal structure	1119:1147	JT-ISH-224 α-2,6-sialyltransferase (Psp2,6ST) was genetically engineered by terminal truncation and site-directed mutagenesis based on its protein crystal structure.					
32236230	10	29	theme	N-glycans	1638:1646	arg1	analysis					1613:1620	2-AB hydrophilic interaction chromatography analysis	1569:1620	2-AB hydrophilic interaction chromatography analysis of the released N-glycans	1569:1646	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	10	29	theme	N-glycans	1638:1646	arg1	characterization					1664:1679	intact mass characterization	1652:1679	intact mass characterization	1652:1679	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	5	30	theme	optimized	849:857	arg1	glycosylation					859:871	optimized glycosylation	849:871	optimized glycosylation	849:871	Herein, a one-pot three-enzyme protocol was developed by engineering a bacterial sialyltransferase to facilitate the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation.					
32236230	12	31	theme	enzymatic	1974:1982	arg1	glycoengineering					1984:1999	the enzymatic glycoengineering	1970:1999	the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies	1970:2078	It provides new insights into engineering bacterial sialyltransferase for adaptation to the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies.					
32236230	10	32	theme	one-pot	1746:1752	arg1	reaction					1767:1774	the optimized one-pot three-enzyme reaction	1732:1774	the optimized one-pot three-enzyme reaction	1732:1774	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	9	33	theme	site-specific	1542:1554	arg1	conjugation					1556:1566	site-specific conjugation	1542:1566	site-specific conjugation	1542:1566	Herceptin and the donor substrate promiscuity allow the introduction of bioorthogonal modifications of N-acetylneuraminic acid into antibodies for site-specific conjugation.					
32236230	8	34	theme	Psp2,6ST	1235:1242	arg1	variants					1244:1251	various Psp2,6ST variants	1227:1251	various Psp2,6ST variants	1227:1251	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	1	35	theme	Terminal	88:95	arg1	glycosylation					143:155	a humanized glycosylation	131:155	a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins	131:225	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	1	35	theme	Terminal	88:95	arg1	α-2,6-sialylation					97:113	Terminal α-2,6-sialylation	88:113	Terminal α-2,6-sialylation of N-glycans	88:126	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	1	36	dep	properties	174:183	arg1	the					170:172	the	170:172	the	170:172	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	3	37	theme	therapeutic	468:478	arg1	development					480:490	therapeutic development	468:490	therapeutic development	468:490	However, the microheterogeneity of N-glycoforms presents a challenge for therapeutic development.					
32236230	5	38	theme	three-enzyme	655:666	arg1	protocol					668:675	a one-pot three-enzyme protocol	645:675	a one-pot three-enzyme protocol	645:675	Herein, a one-pot three-enzyme protocol was developed by engineering a bacterial sialyltransferase to facilitate the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation.					
32236230	5	39	with	antibodies	782:791	arg1	acid					817:820	N-acetylneuraminic acid	798:820	N-acetylneuraminic acid	798:820	Herein, a one-pot three-enzyme protocol was developed by engineering a bacterial sialyltransferase to facilitate the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation.					
32236230	5	39	with	antibodies	782:791	arg1	derivatives					829:839	its derivatives	825:839	its derivatives	825:839	Herein, a one-pot three-enzyme protocol was developed by engineering a bacterial sialyltransferase to facilitate the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation.					
32236230	0	40	theme	bacterial	14:22	arg1	sialyltransferase					24:40	a bacterial sialyltransferase	12:40	a bacterial sialyltransferase for di-sialylation of a therapeutic antibody	12:85	Engineering a bacterial sialyltransferase for di-sialylation of a therapeutic antibody.					
32236230	8	41	theme	truncated	1256:1264	arg1	A235M/A366G					1297:1307	a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G	1254:1307	a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G	1254:1307	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	6	42	from	proficiency	894:904	arg1	remodeling					949:958	antibody remodeling	940:958	antibody remodeling	940:958	To overcome the low proficiency of bacterial sialyltransferase in antibody remodeling, the Photobacterium sp.					
32236230	8	43	theme	Psp2,6ST	1273:1280	arg1	A235M/A366G					1297:1307	a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G	1254:1307	a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G	1254:1307	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	4	44	theme	well-defined	582:593	arg1	antibodies					609:618	well-defined di-sialylated antibodies	582:618	well-defined di-sialylated antibodies	582:618	Therefore, controlled sialylation has drawn considerable attention, but direct access to well-defined di-sialylated antibodies remains limited.					
32236230	10	45	theme	hydrophilic	1574:1584	arg1	analysis					1613:1620	2-AB hydrophilic interaction chromatography analysis	1569:1620	2-AB hydrophilic interaction chromatography analysis of the released N-glycans	1569:1646	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	2	46	theme	other	382:386	arg1	roles					388:392	other roles	382:392	other roles	382:392	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	11	47	theme	IgG	1866:1868	arg1	antibodies					1870:1879	highly di-sialylated IgG antibodies	1845:1879	highly di-sialylated IgG antibodies	1845:1879	This study established a versatile enzymatic approach for producing highly di-sialylated IgG antibodies.					
32236230	11	48	theme	enzymatic	1812:1820	arg1	approach					1822:1829	a versatile enzymatic approach	1800:1829	a versatile enzymatic approach for producing highly di-sialylated IgG antibodies	1800:1879	This study established a versatile enzymatic approach for producing highly di-sialylated IgG antibodies.					
32236230	8	49	theme	-His6	1291:1295	arg1	A235M/A366G					1297:1307	a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G	1254:1307	a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G	1254:1307	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	6	50	theme	Photobacterium	965:978	arg1	sp					980:981	the Photobacterium sp	961:981	the Photobacterium sp	961:981	To overcome the low proficiency of bacterial sialyltransferase in antibody remodeling, the Photobacterium sp.					
32236230	0	51	gly	di-sialylation	46:59	arg1	antibody					78:85	a therapeutic antibody	64:85	a therapeutic antibody	64:85	Engineering a bacterial sialyltransferase for di-sialylation of a therapeutic antibody.					
32236230	7	52	theme	terminal	1060:1067	arg1	truncation					1069:1078	terminal truncation	1060:1078	terminal truncation	1060:1078	JT-ISH-224 α-2,6-sialyltransferase (Psp2,6ST) was genetically engineered by terminal truncation and site-directed mutagenesis based on its protein crystal structure.					
32236230	2	53	dep	di-sialylation	231:244	arg1	N-glycan					261:268	a biantennary N-glycan	247:268	a biantennary N-glycan with two α-2,6-linked sialic acids	247:303	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	10	54	theme	chromatography	1598:1611	arg1	analysis					1613:1620	2-AB hydrophilic interaction chromatography analysis	1569:1620	2-AB hydrophilic interaction chromatography analysis of the released N-glycans	1569:1646	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	1	55	gly	glycoproteins	213:225	arg1	glycoproteins					213:225	therapeutic glycoproteins	201:225	therapeutic glycoproteins	201:225	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	2	56	theme	antibodies	313:322	arg1	di-sialylation					231:244	Fc di-sialylation	228:244	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies	228:322	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	8	57	theme	optimized	1159:1167	arg1	conditions					1178:1187	the optimized reaction conditions	1155:1187	the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants	1155:1251	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	10	58	theme	released	1629:1636	arg1	N-glycans					1638:1646	the released N-glycans	1625:1646	the released N-glycans	1625:1646	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	8	59	theme	catalytic	1346:1354	arg1	efficiency					1356:1365	the catalytic efficiency	1342:1365	the catalytic efficiency of antibody di-sialylation	1342:1392	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	6	60	theme	bacterial	909:917	arg1	sialyltransferase					919:935	bacterial sialyltransferase	909:935	bacterial sialyltransferase	909:935	To overcome the low proficiency of bacterial sialyltransferase in antibody remodeling, the Photobacterium sp.					
32236230	2	61	theme	enhanced	342:349	arg1	activity					369:376	enhanced anti-inflammatory activity	342:376	enhanced anti-inflammatory activity	342:376	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	1	62	theme	therapeutic	201:211	arg1	glycoproteins					213:225	therapeutic glycoproteins	201:225	therapeutic glycoproteins	201:225	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	9	63	theme	donor	1413:1417	arg1	promiscuity					1429:1439	the donor substrate promiscuity	1409:1439	the donor substrate promiscuity	1409:1439	Herceptin and the donor substrate promiscuity allow the introduction of bioorthogonal modifications of N-acetylneuraminic acid into antibodies for site-specific conjugation.					
32236230	8	64	theme	di-sialylation	1379:1392	arg1	efficiency					1356:1365	the catalytic efficiency	1342:1365	the catalytic efficiency of antibody di-sialylation	1342:1392	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	12	65	theme	bacterial	1924:1932	arg1	sialyltransferase					1934:1950	engineering bacterial sialyltransferase	1912:1950	engineering bacterial sialyltransferase	1912:1950	It provides new insights into engineering bacterial sialyltransferase for adaptation to the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies.					
32236230	4	66	theme	controlled	504:513	arg1	sialylation					515:525	controlled sialylation	504:525	controlled sialylation	504:525	Therefore, controlled sialylation has drawn considerable attention, but direct access to well-defined di-sialylated antibodies remains limited.					
32236230	5	67	theme	therapeutic	770:780	arg1	antibodies					782:791	therapeutic antibodies	770:791	therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation	770:871	Herein, a one-pot three-enzyme protocol was developed by engineering a bacterial sialyltransferase to facilitate the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation.					
32236230	2	68	theme	biantennary	249:259	arg1	N-glycan					261:268	a biantennary N-glycan	247:268	a biantennary N-glycan with two α-2,6-linked sialic acids	247:303	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	12	69	theme	new	1894:1896	arg1	insights					1898:1905	new insights	1894:1905	new insights into engineering bacterial sialyltransferase	1894:1950	It provides new insights into engineering bacterial sialyltransferase for adaptation to the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies.					
32236230	2	70	gly	di-sialylation	231:244	arg1	antibodies					313:322	IgG antibodies	309:322	IgG antibodies	309:322	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	10	71	theme	mass	1659:1662	arg1	characterization					1664:1679	intact mass characterization	1652:1679	intact mass characterization	1652:1679	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	12	72	theme	glycosite-specific	2035:2052	arg1	conjugation					2054:2064	the glycosite-specific conjugation	2031:2064	the glycosite-specific conjugation of antibodies	2031:2078	It provides new insights into engineering bacterial sialyltransferase for adaptation to the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies.					
32236230	0	73	theme	antibody	78:85	arg1	di-sialylation					46:59	di-sialylation	46:59	di-sialylation of a therapeutic antibody	46:85	Engineering a bacterial sialyltransferase for di-sialylation of a therapeutic antibody.					
32236230	9	74	theme	modifications	1481:1493	arg1	introduction					1451:1462	the introduction	1447:1462	the introduction of bioorthogonal modifications of N-acetylneuraminic acid into antibodies for site-specific conjugation	1447:1566	Herceptin and the donor substrate promiscuity allow the introduction of bioorthogonal modifications of N-acetylneuraminic acid into antibodies for site-specific conjugation.					
32236230	2	75	theme	α-2,6-linked	279:290	arg1	acids					299:303	two α-2,6-linked sialic acids	275:303	two α-2,6-linked sialic acids	275:303	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	4	76	gly	di-sialylated	595:607	arg1	antibodies					609:618	well-defined di-sialylated antibodies	582:618	well-defined di-sialylated antibodies	582:618	Therefore, controlled sialylation has drawn considerable attention, but direct access to well-defined di-sialylated antibodies remains limited.					
32236230	4	77	theme	direct	565:570	arg1	access					572:577	direct access	565:577	direct access to well-defined di-sialylated antibodies	565:618	Therefore, controlled sialylation has drawn considerable attention, but direct access to well-defined di-sialylated antibodies remains limited.					
32236230	1	78	gly	α-2,6-sialylation	97:113	arg1	N-glycans					118:126	N-glycans	118:126	N-glycans	118:126	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	11	79	gly	di-sialylated	1852:1864	arg1	antibodies					1870:1879	highly di-sialylated IgG antibodies	1845:1879	highly di-sialylated IgG antibodies	1845:1879	This study established a versatile enzymatic approach for producing highly di-sialylated IgG antibodies.					
32236230	9	80	theme	N-acetylneuraminic	1498:1515	arg1	acid					1517:1520	N-acetylneuraminic acid	1498:1520	N-acetylneuraminic acid	1498:1520	Herceptin and the donor substrate promiscuity allow the introduction of bioorthogonal modifications of N-acetylneuraminic acid into antibodies for site-specific conjugation.					
32236230	12	81	theme	therapeutic	2004:2014	arg1	antibodies					2016:2025	therapeutic antibodies	2004:2025	therapeutic antibodies	2004:2025	It provides new insights into engineering bacterial sialyltransferase for adaptation to the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies.					
32236230	8	82	theme	various	1227:1233	arg1	variants					1244:1251	various Psp2,6ST variants	1227:1251	various Psp2,6ST variants	1227:1251	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	4	83	theme	considerable	537:548	arg1	attention					550:558	considerable attention	537:558	considerable attention	537:558	Therefore, controlled sialylation has drawn considerable attention, but direct access to well-defined di-sialylated antibodies remains limited.					
32236230	10	84	theme	high	1695:1698	arg1	di-sialylation					1700:1713	the high di-sialylation	1691:1713	the high di-sialylation of Herceptin	1691:1726	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	8	85	theme	variants	1244:1251	arg1	screening					1214:1222	activity-based screening	1199:1222	activity-based screening of various Psp2,6ST variants	1199:1251	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	7	86	theme	protein	1123:1129	arg1	structure					1139:1147	its protein crystal structure	1119:1147	its protein crystal structure	1119:1147	JT-ISH-224 α-2,6-sialyltransferase (Psp2,6ST) was genetically engineered by terminal truncation and site-directed mutagenesis based on its protein crystal structure.					
32236230	10	87	theme	optimized	1736:1744	arg1	reaction					1767:1774	the optimized one-pot three-enzyme reaction	1732:1774	the optimized one-pot three-enzyme reaction	1732:1774	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	8	88	dep	conditions	1178:1187	arg1	using					1193:1197	using	1193:1197	using activity-based screening of various Psp2,6ST variants	1193:1251	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	12	89	theme	engineering	1912:1922	arg1	sialyltransferase					1934:1950	engineering bacterial sialyltransferase	1912:1950	engineering bacterial sialyltransferase	1912:1950	It provides new insights into engineering bacterial sialyltransferase for adaptation to the enzymatic glycoengineering of therapeutic antibodies and the glycosite-specific conjugation of antibodies.					
32236230	3	90	gly	microheterogeneity	408:425	arg1	N-glycoforms					430:441	N-glycoforms	430:441	N-glycoforms	430:441	However, the microheterogeneity of N-glycoforms presents a challenge for therapeutic development.					
32236230	5	91	theme	one-pot	647:653	arg1	protocol					668:675	a one-pot three-enzyme protocol	645:675	a one-pot three-enzyme protocol	645:675	Herein, a one-pot three-enzyme protocol was developed by engineering a bacterial sialyltransferase to facilitate the modification of therapeutic antibodies with N-acetylneuraminic acid or its derivatives towards optimized glycosylation.					
32236230	3	92	theme	N-glycoforms	430:441	arg1	microheterogeneity					408:425	the microheterogeneity	404:425	the microheterogeneity of N-glycoforms	404:441	However, the microheterogeneity of N-glycoforms presents a challenge for therapeutic development.					
32236230	8	93	theme	mutant	1266:1271	arg1	A235M/A366G					1297:1307	a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G	1254:1307	a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G	1254:1307	With the optimized reaction conditions and using activity-based screening of various Psp2,6ST variants, a truncated mutant Psp2,6ST (111-511)-His6 A235M/A366G was shown to effectively improve the catalytic efficiency of antibody di-sialylation.					
32236230	1	94	theme	N-glycans	118:126	arg1	glycosylation					143:155	a humanized glycosylation	131:155	a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins	131:225	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	1	94	theme	N-glycans	118:126	arg1	α-2,6-sialylation					97:113	Terminal α-2,6-sialylation	88:113	Terminal α-2,6-sialylation of N-glycans	88:126	Terminal α-2,6-sialylation of N-glycans is a humanized glycosylation that affects the properties and efficacy of therapeutic glycoproteins.					
32236230	4	95	theme	di-sialylated	595:607	arg1	antibodies					609:618	well-defined di-sialylated antibodies	582:618	well-defined di-sialylated antibodies	582:618	Therefore, controlled sialylation has drawn considerable attention, but direct access to well-defined di-sialylated antibodies remains limited.					
32236230	10	96	theme	three-enzyme	1754:1765	arg1	reaction					1767:1774	the optimized one-pot three-enzyme reaction	1732:1774	the optimized one-pot three-enzyme reaction	1732:1774	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	6	97	theme	low	890:892	arg1	proficiency					894:904	the low proficiency	886:904	the low proficiency of bacterial sialyltransferase in antibody remodeling	886:958	To overcome the low proficiency of bacterial sialyltransferase in antibody remodeling, the Photobacterium sp.					
32236230	10	98	theme	2-AB	1569:1572	arg1	analysis					1613:1620	2-AB hydrophilic interaction chromatography analysis	1569:1620	2-AB hydrophilic interaction chromatography analysis of the released N-glycans	1569:1646	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
32236230	11	99	theme	di-sialylated	1852:1864	arg1	antibodies					1870:1879	highly di-sialylated IgG antibodies	1845:1879	highly di-sialylated IgG antibodies	1845:1879	This study established a versatile enzymatic approach for producing highly di-sialylated IgG antibodies.					
32236230	2	100	theme	Fc	228:229	arg1	di-sialylation					231:244	Fc di-sialylation	228:244	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies	228:322	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	2	101	theme	anti-inflammatory	351:367	arg1	activity					369:376	enhanced anti-inflammatory activity	342:376	enhanced anti-inflammatory activity	342:376	Fc di-sialylation (a biantennary N-glycan with two α-2,6-linked sialic acids) of IgG antibodies imparts them with enhanced anti-inflammatory activity and other roles.					
32236230	10	102	theme	interaction	1586:1596	arg1	analysis					1613:1620	2-AB hydrophilic interaction chromatography analysis	1569:1620	2-AB hydrophilic interaction chromatography analysis of the released N-glycans	1569:1646	2-AB hydrophilic interaction chromatography analysis of the released N-glycans and intact mass characterization confirmed the high di-sialylation of Herceptin via the optimized one-pot three-enzyme reaction.					
33459939	9	0	theme	rationalized	1724:1735	arg1	development					1744:1754	a more rationalized method development	1717:1754	a more rationalized method development	1717:1754	These insights allow a more rationalized method development for linkage-specific sialic derivatization in the future.					
33459939	7	1	theme	acids	1490:1494	arg1	amidation					1458:1466	the efficient, linkage-specific amidation	1426:1466	the efficient, linkage-specific amidation of α2,3-linked sialic acids	1426:1494	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	9	2	theme	linkage-specific	1760:1775	arg1	derivatization					1784:1797	linkage-specific sialic derivatization	1760:1797	linkage-specific sialic derivatization in the future	1760:1811	These insights allow a more rationalized method development for linkage-specific sialic derivatization in the future.					
33459939	7	3	theme	α2,3-linked	1471:1481	arg1	acids					1490:1494	α2,3-linked sialic acids	1471:1494	α2,3-linked sialic acids	1471:1494	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	3	4	theme	α2,3-linked	570:580	arg1	acids					589:593	α2,3-linked sialic acids	570:593	α2,3-linked sialic acids	570:593	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	6	5	theme	complex	1271:1277	arg1	standards					1286:1294	simple and complex glycan standards	1260:1294	simple and complex glycan standards	1260:1294	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	3	6	theme	subterminal	613:623	arg1	monosaccharides					625:639	the subterminal monosaccharides	609:639	the subterminal monosaccharides	609:639	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	7	7	dep	efficient	1430:1438	arg1	linkage-specific					1441:1456	linkage-specific	1441:1456	linkage-specific	1441:1456	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	1	8	theme	diverse	231:237	arg1	roles					250:254	diverse biological roles	231:254	diverse biological roles	231:254	Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles.					
33459939	6	9	theme	reaction	1237:1244	arg1	conditions					1246:1255	controlled reaction conditions	1226:1255	controlled reaction conditions	1226:1255	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	6	10	link	α2,3-linked	1189:1199	arg1	acids					1208:1212	α2,3-linked sialic acids	1189:1212	α2,3-linked sialic acids	1189:1212	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	6	11	theme	acids	1208:1212	arg1	amidation					1176:1184	the amidation	1172:1184	the amidation of α2,3-linked sialic acids	1172:1212	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	5	12	theme	sialic	963:968	arg1	acids					970:974	α2,3-linked sialic acids	951:974	α2,3-linked sialic acids	951:974	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	7	13	theme	lactone	1386:1392	arg1	formation					1394:1402	prior lactone formation	1380:1402	prior lactone formation	1380:1402	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	7	13	theme	lactone	1386:1392	arg1	prerequisite					1409:1420	a prerequisite	1407:1420	a prerequisite	1407:1420	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	4	14	theme	second	728:733	arg1	step					735:738	a second step	726:738	a second step	726:738	In a second step, the lactones are converted into amide derivatives.					
33459939	0	15	theme	linkage-specific	51:66	arg1	reactions					95:103	linkage-specific sialic acid derivatization reactions	51:103	linkage-specific sialic acid derivatization reactions	51:103	The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions.					
33459939	3	16	theme	sialic	582:587	arg1	acids					589:593	α2,3-linked sialic acids	570:593	α2,3-linked sialic acids	570:593	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	2	17	theme	sialic	274:279	arg1	acid					281:284	Linkage-specific sialic acid	257:284	Linkage-specific sialic acid characterization	257:301	Linkage-specific sialic acid characterization is increasingly performed by mass spectrometry, aided by differential sialic acid derivatization to discriminate between linkage isomers.					
33459939	1	18	from	glycans	186:192	arg1	α2,6-linkage					213:224	α2,6-linkage	213:224	α2,6-linkage	213:224	Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles.					
33459939	1	18	from	glycans	186:192	arg1	α2,3-					204:208	α2,3-	204:208	α2,3-	204:208	Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles.					
33459939	0	19	theme	acid	75:78	arg1	reactions					95:103	linkage-specific sialic acid derivatization reactions	51:103	linkage-specific sialic acid derivatization reactions	51:103	The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions.					
33459939	7	20	link	α2,3-linked	1471:1481	arg1	acids					1490:1494	α2,3-linked sialic acids	1471:1494	α2,3-linked sialic acids	1471:1494	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	3	21	theme	sialic	677:682	arg1	acids					684:688	α2,6-linked sialic acids	665:688	α2,6-linked sialic acids	665:688	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	1	22	theme	vertebrate	149:158	arg1	glycomes					160:167	vertebrate glycomes	149:167	vertebrate glycomes	149:167	Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles.					
33459939	3	23	theme	derivatization	482:495	arg1	reactions					497:505	such derivatization reactions	477:505	such derivatization reactions	477:505	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	5	24	theme	lactone	1013:1019	arg1	aminolysis					995:1004	direct aminolysis	988:1004	direct aminolysis of the lactone	988:1019	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	5	24	theme	lactone	1013:1019	arg1	amidation					1068:1076	subsequent amidation	1057:1076	subsequent amidation	1057:1076	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	7	25	theme	prior	1380:1384	arg1	formation					1394:1402	prior lactone formation	1380:1402	prior lactone formation	1380:1402	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	7	25	theme	prior	1380:1384	arg1	prerequisite					1409:1420	a prerequisite	1407:1420	a prerequisite	1407:1420	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	4	26	theme	amide	773:777	arg1	derivatives					779:789	amide derivatives	773:789	amide derivatives	773:789	In a second step, the lactones are converted into amide derivatives.					
33459939	5	27	theme	intermediates	839:851	arg1	role					819:822	role	819:822	role	819:822	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	5	27	theme	intermediates	839:851	arg1	structure					805:813	structure	805:813	structure	805:813	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	6	28	used	used	1098:1101	arg2	we					1095:1096	we	1095:1096	we	1095:1096	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	7	29	from	prerequisite	1409:1420	arg1	protocols					1370:1378	common sialic acid derivatization protocols	1336:1378	common sialic acid derivatization protocols	1336:1378	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	7	30	theme	derivatization	1355:1368	arg1	protocols					1370:1378	common sialic acid derivatization protocols	1336:1378	common sialic acid derivatization protocols	1336:1378	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	3	31	link	α2,6-linked	665:675	arg1	acids					684:688	α2,6-linked sialic acids	665:688	α2,6-linked sialic acids	665:688	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	6	32	theme	α2,3-linked	1189:1199	arg1	acids					1208:1212	α2,3-linked sialic acids	1189:1212	α2,3-linked sialic acids	1189:1212	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	5	33	theme	direct	988:993	arg1	aminolysis					995:1004	direct aminolysis	988:1004	direct aminolysis of the lactone	988:1019	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	7	34	theme	sialic	1343:1348	arg1	protocols					1370:1378	common sialic acid derivatization protocols	1336:1378	common sialic acid derivatization protocols	1336:1378	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	3	35	theme	first	463:467	arg1	step					469:472	the first step	459:472	the first step of such derivatization reactions	459:505	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	8	36	theme	lactone	1636:1642	arg1	intermediate					1644:1655	exclusively the C2 lactone intermediate	1617:1655	exclusively the C2 lactone intermediate	1617:1655	Furthermore, nuclear magnetic resonance spectroscopy confirmed that exclusively the C2 lactone intermediate is formed on a sialyllactose standard.					
33459939	6	37	from	role	1136:1139	arg1	amidation					1176:1184	the amidation	1172:1184	the amidation of α2,3-linked sialic acids	1172:1212	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	3	38	theme	carboxyl	529:536	arg1	activator					544:552	a carboxyl group activator	527:552	a carboxyl group activator	527:552	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	0	39	theme	intermediates	34:46	arg1	role					18:21	role	18:21	role	18:21	The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions.					
33459939	0	39	theme	intermediates	34:46	arg1	structure					4:12	structure	4:12	structure	4:12	The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions.					
33459939	8	40	theme	nuclear	1562:1568	arg1	resonance					1579:1587	nuclear magnetic resonance	1562:1587	nuclear magnetic resonance spectroscopy	1562:1600	Furthermore, nuclear magnetic resonance spectroscopy confirmed that exclusively the C2 lactone intermediate is formed on a sialyllactose standard.					
33459939	2	41	theme	sialic	373:378	arg1	acid					380:383	differential sialic acid	360:383	differential sialic acid derivatization	360:398	Linkage-specific sialic acid characterization is increasingly performed by mass spectrometry, aided by differential sialic acid derivatization to discriminate between linkage isomers.					
33459939	1	42	theme	biological	239:248	arg1	roles					250:254	diverse biological roles	231:254	diverse biological roles	231:254	Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles.					
33459939	2	43	theme	linkage	424:430	arg1	isomers					432:438	linkage isomers	424:438	linkage isomers	424:438	Linkage-specific sialic acid characterization is increasingly performed by mass spectrometry, aided by differential sialic acid derivatization to discriminate between linkage isomers.					
33459939	5	44	theme	lactone	1034:1040	arg1	hydrolysis					1042:1051	lactone hydrolysis	1034:1051	lactone hydrolysis	1034:1051	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	3	45	theme	activator	544:552	arg1	presence					515:522	the presence	511:522	the presence of a carboxyl group activator and a catalyst	511:567	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	7	46	theme	direct	1530:1535	arg1	aminolysis					1537:1546	direct aminolysis	1530:1546	direct aminolysis	1530:1546	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	9	47	theme	method	1737:1742	arg1	development					1744:1754	a more rationalized method development	1717:1754	a more rationalized method development	1717:1754	These insights allow a more rationalized method development for linkage-specific sialic derivatization in the future.					
33459939	2	48	theme	mass	332:335	arg1	spectrometry					337:348	mass spectrometry	332:348	mass spectrometry	332:348	Linkage-specific sialic acid characterization is increasingly performed by mass spectrometry, aided by differential sialic acid derivatization to discriminate between linkage isomers.					
33459939	6	49	theme	sialic	1201:1206	arg1	acids					1208:1212	α2,3-linked sialic acids	1189:1212	α2,3-linked sialic acids	1189:1212	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	9	50	theme	sialic	1777:1782	arg1	derivatization					1784:1797	linkage-specific sialic derivatization	1760:1797	linkage-specific sialic derivatization in the future	1760:1811	These insights allow a more rationalized method development for linkage-specific sialic derivatization in the future.					
33459939	7	51	theme	sialic	1483:1488	arg1	acids					1490:1494	α2,3-linked sialic acids	1471:1494	α2,3-linked sialic acids	1471:1494	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	6	52	theme	glycan	1279:1284	arg1	standards					1286:1294	simple and complex glycan standards	1260:1294	simple and complex glycan standards	1260:1294	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	8	53	theme	resonance	1579:1587	arg1	spectroscopy					1589:1600	nuclear magnetic resonance spectroscopy	1562:1600	nuclear magnetic resonance spectroscopy	1562:1600	Furthermore, nuclear magnetic resonance spectroscopy confirmed that exclusively the C2 lactone intermediate is formed on a sialyllactose standard.					
33459939	9	54	from	derivatization	1784:1797	arg1	future					1806:1811	future	1806:1811	future	1806:1811	These insights allow a more rationalized method development for linkage-specific sialic derivatization in the future.					
33459939	5	55	theme	reported	860:867	arg1	reactions					869:877	the reported reactions	856:877	the reported reactions	856:877	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	5	56	dep	structure	805:813	arg1	the					801:803	the	801:803	the	801:803	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	6	57	theme	intermediate	1156:1167	arg1	role					1136:1139	the role	1132:1139	the role of the lactone intermediate in the amidation of α2,3-linked sialic acids	1132:1212	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	2	58	theme	acid	380:383	arg1	derivatization					385:398	differential sialic acid derivatization	360:398	differential sialic acid derivatization	360:398	Linkage-specific sialic acid characterization is increasingly performed by mass spectrometry, aided by differential sialic acid derivatization to discriminate between linkage isomers.					
33459939	2	59	theme	Linkage-specific	257:272	arg1	acid					281:284	Linkage-specific sialic acid	257:284	Linkage-specific sialic acid characterization	257:301	Linkage-specific sialic acid characterization is increasingly performed by mass spectrometry, aided by differential sialic acid derivatization to discriminate between linkage isomers.					
33459939	7	60	theme	efficient	1430:1438	arg1	amidation					1458:1466	the efficient, linkage-specific amidation	1426:1466	the efficient, linkage-specific amidation of α2,3-linked sialic acids	1426:1494	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	8	61	theme	magnetic	1570:1577	arg1	resonance					1579:1587	nuclear magnetic resonance	1562:1587	nuclear magnetic resonance spectroscopy	1562:1600	Furthermore, nuclear magnetic resonance spectroscopy confirmed that exclusively the C2 lactone intermediate is formed on a sialyllactose standard.					
33459939	5	62	from	structure	805:813	arg1	reactions					869:877	the reported reactions	856:877	the reported reactions	856:877	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	3	63	theme	α2,6-linked	665:675	arg1	acids					684:688	α2,6-linked sialic acids	665:688	α2,6-linked sialic acids	665:688	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	5	64	from	reactions	869:877	arg1	role					819:822	role	819:822	role	819:822	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	5	64	from	reactions	869:877	arg1	structure					805:813	structure	805:813	structure	805:813	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	5	65	theme	α2,3-linked	951:961	arg1	acids					970:974	α2,3-linked sialic acids	951:974	α2,3-linked sialic acids	951:974	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	6	66	theme	controlled	1226:1235	arg1	conditions					1246:1255	controlled reaction conditions	1226:1255	controlled reaction conditions	1226:1255	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	0	67	theme	sialic	68:73	arg1	reactions					95:103	linkage-specific sialic acid derivatization reactions	51:103	linkage-specific sialic acid derivatization reactions	51:103	The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions.					
33459939	2	68	theme	acid	281:284	arg1	characterization					286:301	Linkage-specific sialic acid characterization	257:301	Linkage-specific sialic acid characterization	257:301	Linkage-specific sialic acid characterization is increasingly performed by mass spectrometry, aided by differential sialic acid derivatization to discriminate between linkage isomers.					
33459939	0	69	from	role	18:21	arg1	reactions					95:103	linkage-specific sialic acid derivatization reactions	51:103	linkage-specific sialic acid derivatization reactions	51:103	The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions.					
33459939	0	70	theme	derivatization	80:93	arg1	reactions					95:103	linkage-specific sialic acid derivatization reactions	51:103	linkage-specific sialic acid derivatization reactions	51:103	The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions.					
33459939	0	71	from	structure	4:12	arg1	reactions					95:103	linkage-specific sialic acid derivatization reactions	51:103	linkage-specific sialic acid derivatization reactions	51:103	The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions.					
33459939	5	72	from	role	819:822	arg1	reactions					869:877	the reported reactions	856:877	the reported reactions	856:877	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	3	73	link	α2,3-linked	570:580	arg1	acids					589:593	α2,3-linked sialic acids	570:593	α2,3-linked sialic acids	570:593	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	6	74	theme	mass	1103:1106	arg1	spectrometry					1108:1119	mass spectrometry	1103:1119	mass spectrometry	1103:1119	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	3	75	theme	reactions	497:505	arg1	step					469:472	the first step	459:472	the first step of such derivatization reactions	459:505	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	5	76	theme	lactone	831:837	arg1	intermediates					839:851	the lactone intermediates	827:851	the lactone intermediates in the reported reactions	827:877	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	5	77	from	intermediates	839:851	arg1	reactions					869:877	the reported reactions	856:877	the reported reactions	856:877	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	5	78	theme	acids	970:974	arg1	amidation					938:946	the amidation	934:946	the amidation of α2,3-linked sialic acids	934:974	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	7	79	theme	acid	1350:1353	arg1	protocols					1370:1378	common sialic acid derivatization protocols	1336:1378	common sialic acid derivatization protocols	1336:1378	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	8	80	theme	C2	1633:1634	arg1	intermediate					1644:1655	exclusively the C2 lactone intermediate	1617:1655	exclusively the C2 lactone intermediate	1617:1655	Furthermore, nuclear magnetic resonance spectroscopy confirmed that exclusively the C2 lactone intermediate is formed on a sialyllactose standard.					
33459939	3	81	theme	such	477:480	arg1	reactions					497:505	such derivatization reactions	477:505	such derivatization reactions	477:505	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	1	82	theme	Sialic	106:111	arg1	acids					113:117	Sialic acids	106:117	Sialic acids	106:117	Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles.					
33459939	7	83	theme	common	1336:1341	arg1	protocols					1370:1378	common sialic acid derivatization protocols	1336:1378	common sialic acid derivatization protocols	1336:1378	The results unambiguously show that in common sialic acid derivatization protocols prior lactone formation is a prerequisite for the efficient, linkage-specific amidation of α2,3-linked sialic acids, which proceeds predominantly via direct aminolysis.					
33459939	3	84	theme	amide	695:699	arg1	derivatives					710:720	amide or ester derivatives	695:720	derivatives	710:720	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	0	85	theme	lactone	26:32	arg1	intermediates					34:46	lactone intermediates	26:46	lactone intermediates	26:46	The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions.					
33459939	5	86	link	α2,3-linked	951:961	arg1	acids					970:974	α2,3-linked sialic acids	951:974	α2,3-linked sialic acids	951:974	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	5	87	theme	subsequent	1057:1066	arg1	amidation					1068:1076	subsequent amidation	1057:1076	subsequent amidation	1057:1076	Notably, the structure and role of the lactone intermediates in the reported reactions remained ambiguous, leaving it unclear to which extent the amidation of α2,3-linked sialic acids depended on direct aminolysis of the lactone, rather than lactone hydrolysis and subsequent amidation.					
33459939	6	88	theme	lactone	1148:1154	arg1	intermediate					1156:1167	the lactone intermediate	1144:1167	the lactone intermediate	1144:1167	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	3	89	theme	group	538:542	arg1	activator					544:552	a carboxyl group activator	527:552	a carboxyl group activator	527:552	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	1	90	with	glycans	186:192	arg1	roles					250:254	diverse biological roles	231:254	diverse biological roles	231:254	Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles.					
33459939	6	91	theme	simple	1260:1265	arg1	standards					1286:1294	simple and complex glycan standards	1260:1294	simple and complex glycan standards	1260:1294	In this report, we used mass spectrometry to unravel the role of the lactone intermediate in the amidation of α2,3-linked sialic acids by applying controlled reaction conditions on simple and complex glycan standards.					
33459939	8	92	theme	standard	1686:1693	arg1	sialyllactose					1672:1684	a sialyllactose standard	1670:1693	a sialyllactose standard	1670:1693	Furthermore, nuclear magnetic resonance spectroscopy confirmed that exclusively the C2 lactone intermediate is formed on a sialyllactose standard.					
33459939	3	93	theme	ester	704:708	arg1	derivatives					710:720	amide or ester derivatives	695:720	derivatives	710:720	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
33459939	2	94	theme	differential	360:371	arg1	acid					380:383	differential sialic acid	360:383	differential sialic acid derivatization	360:398	Linkage-specific sialic acid characterization is increasingly performed by mass spectrometry, aided by differential sialic acid derivatization to discriminate between linkage isomers.					
33459939	0	95	dep	structure	4:12	arg1	The					0:2	The	0:2	The	0:2	The structure and role of lactone intermediates in linkage-specific sialic acid derivatization reactions.					
33459939	3	96	theme	catalyst	560:567	arg1	presence					515:522	the presence	511:522	the presence of a carboxyl group activator and a catalyst	511:567	Typically, during the first step of such derivatization reactions, in the presence of a carboxyl group activator and a catalyst, α2,3-linked sialic acids condense with the subterminal monosaccharides to form lactones, while α2,6-linked sialic acids form amide or ester derivatives.					
32005658	8	0	theme	catalytic	1713:1721	arg1	residues					1733:1740	two catalytic glutamate residues	1709:1740	two catalytic glutamate residues conserved across glycosidases	1709:1770	Moreover, the introduction of two catalytic glutamate residues conserved across glycosidases into sKlotho enhanced its glucuronidase activity but decreased its FGF23 co-receptor activity, suggesting that these two functions might be structurally divergent.					
32005658	4	1	theme	variants	855:862	arg1	relationship					794:805	structure-function relationship	775:805	structure-function relationship	775:805	Here, we explored the structure-function relationship and post-translational modifications of sKlotho variants to guide the future design of sKlotho-based therapeutics.					
32005658	4	1	theme	variants	855:862	arg1	modifications					830:842	post-translational modifications	811:842	post-translational modifications	811:842	Here, we explored the structure-function relationship and post-translational modifications of sKlotho variants to guide the future design of sKlotho-based therapeutics.					
32005658	3	2	theme	sKlotho	663:669	arg1	supplementation					671:685	sKlotho supplementation	663:685	sKlotho supplementation	663:685	Low sKlotho in CKD is associated with disease progression, and sKlotho supplementation has emerged as a potential therapeutic strategy for managing CKD.					
32005658	8	3	theme	residues	1733:1740	arg1	introduction					1693:1704	the introduction	1689:1704	the introduction of two catalytic glutamate residues conserved across glycosidases into sKlotho	1689:1783	Moreover, the introduction of two catalytic glutamate residues conserved across glycosidases into sKlotho enhanced its glucuronidase activity but decreased its FGF23 co-receptor activity, suggesting that these two functions might be structurally divergent.					
32005658	5	4	theme	co-receptor	1191:1201	arg1	activity					1203:1210	its co-receptor activity	1187:1210	its co-receptor activity	1187:1210	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	6	5	theme	multiple	1449:1456	arg1	sites					1467:1471	multiple N-linked sites	1449:1471	multiple N-linked sites	1449:1471	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	6	6	theme	transfer	1532:1539	arg1	motif					1541:1545	a putative GalNAc transfer motif	1514:1545	a putative GalNAc transfer motif	1514:1545	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	4	7	theme	post-translational	811:828	arg1	modifications					830:842	post-translational modifications	811:842	post-translational modifications	811:842	Here, we explored the structure-function relationship and post-translational modifications of sKlotho variants to guide the future design of sKlotho-based therapeutics.					
32005658	6	8	theme	putative	1516:1523	arg1	motif					1541:1545	a putative GalNAc transfer motif	1514:1545	a putative GalNAc transfer motif	1514:1545	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	0	9	theme	kidney	128:133	arg1	disease					135:141	managing kidney disease	119:141	managing kidney disease	119:141	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	5	10	theme	varied	1016:1021	arg1	activities					1023:1032	varied activities	1016:1032	varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold	1016:1217	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	4	11	theme	therapeutics	908:919	arg1	design					884:889	the future design	873:889	the future design of sKlotho-based therapeutics	873:919	Here, we explored the structure-function relationship and post-translational modifications of sKlotho variants to guide the future design of sKlotho-based therapeutics.					
32005658	9	12	theme	sKlotho	2028:2034	arg1	therapeutics					2036:2047	pharmacologically enhanced sKlotho therapeutics	2001:2047	pharmacologically enhanced sKlotho therapeutics	2001:2047	These findings open up opportunities for rational engineering of pharmacologically enhanced sKlotho therapeutics for managing kidney disease.					
32005658	1	13	from	deletion	255:262	arg1	mice					286:289	mice	286:289	mice	286:289	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	8	14	theme	glucuronidase	1798:1810	arg1	activity					1812:1819	its glucuronidase activity	1794:1819	its glucuronidase activity	1794:1819	Moreover, the introduction of two catalytic glutamate residues conserved across glycosidases into sKlotho enhanced its glucuronidase activity but decreased its FGF23 co-receptor activity, suggesting that these two functions might be structurally divergent.					
32005658	6	15	mod	modified	1356:1363	arg1	HEK-sKlotho					1332:1342	HEK-sKlotho	1332:1342	HEK-sKlotho	1332:1342	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	6	15	mod	modified	1356:1363	arg3	structure					1390:1398	an unusual N-glycan structure	1370:1398	an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif	1370:1545	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	2	16	theme	glycosidase	529:539	arg1	activity					541:548	glycosidase activity	529:548	glycosidase activity	529:548	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	3	17	theme	potential	704:712	arg1	strategy					726:733	a potential therapeutic strategy	702:733	a potential therapeutic strategy for managing CKD	702:750	Low sKlotho in CKD is associated with disease progression, and sKlotho supplementation has emerged as a potential therapeutic strategy for managing CKD.					
32005658	6	18	theme	glycopeptide-mapping	1288:1307	arg1	analyses					1309:1316	MS and glycopeptide-mapping analyses	1281:1316	analyses	1309:1316	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	5	19	theme	WT	992:993	arg1	proteins					1003:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins	922:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins	922:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	7	20	dep	mutagenesis	1562:1572	arg1	analyses					1598:1605	analyses	1598:1605	analyses	1598:1605	Site-directed mutagenesis and structural modeling analyses directly implicated N-glycans in Klotho's protein folding and function.					
32005658	4	21	theme	future	877:882	arg1	design					884:889	the future design	873:889	the future design of sKlotho-based therapeutics	873:919	Here, we explored the structure-function relationship and post-translational modifications of sKlotho variants to guide the future design of sKlotho-based therapeutics.					
32005658	9	22	theme	kidney	2062:2067	arg1	disease					2069:2075	kidney disease	2062:2075	kidney disease	2062:2075	These findings open up opportunities for rational engineering of pharmacologically enhanced sKlotho therapeutics for managing kidney disease.					
32005658	5	23	theme	hyposialylated	1253:1266	arg1	HEK-sKlotho					1268:1278	hyposialylated HEK-sKlotho	1253:1278	hyposialylated HEK-sKlotho	1253:1278	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	6	24	theme	MS	1281:1282	arg1	analyses					1309:1316	MS and glycopeptide-mapping analyses	1281:1316	analyses	1309:1316	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	6	25	theme	sites	1467:1471	arg1	one					1474:1476	one	1474:1476	one	1474:1476	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	6	25	theme	sites	1467:1471	arg1	sites					1467:1471	multiple N-linked sites	1449:1471	multiple N-linked sites	1449:1471	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	0	26	theme	antiaging	60:68	arg1	Klotho					78:83	the antiaging protein Klotho	56:83	the antiaging protein Klotho	56:83	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	5	27	theme	in	1082:1083	arg1	co-receptor					1043:1053	FGF23 co-receptor and β-glucuronidase assays	1037:1080	co-receptor	1043:1053	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	8	28	theme	co-receptor	1845:1855	arg1	activity					1857:1864	its FGF23 co-receptor activity	1835:1864	its FGF23 co-receptor activity	1835:1864	Moreover, the introduction of two catalytic glutamate residues conserved across glycosidases into sKlotho enhanced its glucuronidase activity but decreased its FGF23 co-receptor activity, suggesting that these two functions might be structurally divergent.					
32005658	1	29	theme	discovered	161:170	arg1	protein					191:197	The fortuitously discovered antiaging membrane protein αKlotho	144:205	The fortuitously discovered antiaging membrane protein αKlotho (Klotho)	144:214	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	1	29	theme	discovered	161:170	arg1	Klotho					208:213	Klotho	208:213	Klotho	208:213	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	0	30	theme	Klotho	78:83	arg1	form					48:51	the soluble form	36:51	the soluble form of the antiaging protein Klotho	36:83	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	1	31	theme	membrane	182:189	arg1	protein					191:197	The fortuitously discovered antiaging membrane protein αKlotho	144:205	The fortuitously discovered antiaging membrane protein αKlotho (Klotho)	144:214	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	1	31	theme	membrane	182:189	arg1	Klotho					208:213	Klotho	208:213	Klotho	208:213	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	7	32	theme	structural	1578:1587	arg1	modeling					1589:1596	structural modeling	1578:1596	structural modeling	1578:1596	Site-directed mutagenesis and structural modeling analyses directly implicated N-glycans in Klotho's protein folding and function.					
32005658	0	33	theme	therapeutic	90:100	arg1	implications					102:113	therapeutic implications	90:113	therapeutic implications for managing kidney disease	90:141	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	1	34	theme	chronic	340:346	arg1	CKD					364:366	CKD	364:366	CKD	364:366	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	1	34	theme	chronic	340:346	arg1	disease					355:361	chronic kidney disease	340:361	chronic kidney disease (CKD)	340:367	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	2	35	theme	extracellular	472:484	arg1	factor					564:569	a humoral factor	554:569	a humoral factor that regulates renal health	554:597	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	35	theme	extracellular	472:484	arg1	domain					486:491	its shed extracellular domain	463:491	its shed extracellular domain	463:491	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	35	theme	extracellular	472:484	arg1	Klotho					502:507	soluble Klotho	494:507	soluble Klotho (sKlotho)	494:517	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	36	theme	renal	586:590	arg1	health					592:597	renal health	586:597	renal health	586:597	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	5	37	from	activities	1023:1032	arg1	properties					1104:1113	distinct properties	1095:1113	distinct properties	1095:1113	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	37	from	activities	1023:1032	arg1	assays					1075:1080	FGF23 co-receptor and β-glucuronidase assays	1037:1080	assays	1075:1080	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	37	from	activities	1023:1032	arg1	co-receptor					1043:1053	FGF23 co-receptor and β-glucuronidase assays	1037:1080	co-receptor	1043:1053	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	38	theme	β-glucuronidase	1059:1073	arg1	assays					1075:1080	FGF23 co-receptor and β-glucuronidase assays	1037:1080	assays	1075:1080	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	0	39	theme	form	48:51	arg1	relationships					19:31	Structure-function relationships	0:31	Structure-function relationships of the soluble form of the antiaging protein Klotho	0:83	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	2	40	theme	factor	426:431	arg1	signaling					444:452	fibroblast growth factor 23 (FGF23) signaling	408:452	fibroblast growth factor 23 (FGF23) signaling	408:452	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	5	41	dep	in	1115:1116	arg1	vivo					1118:1121	vivo	1118:1121	vivo	1118:1121	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	0	42	theme	Structure-function	0:17	arg1	relationships					19:31	Structure-function relationships	0:31	Structure-function relationships of the soluble form of the antiaging protein Klotho	0:83	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	1	43	theme	Klotho	271:276	arg1	gene					278:281	the Klotho gene	267:281	the Klotho gene	267:281	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	5	44	theme	Chinese	922:928	arg1	CHO					945:947	CHO	945:947	CHO	945:947	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	44	theme	Chinese	922:928	arg1	ovary					938:942	Chinese hamster ovary	922:942	Chinese hamster ovary	922:942	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	2	45	theme	fibroblast	408:417	arg1	factor					426:431	fibroblast growth factor 23	408:434	fibroblast growth factor 23 (FGF23) signaling	408:452	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	45	theme	fibroblast	408:417	arg1	FGF23					437:441	FGF23	437:441	FGF23	437:441	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	5	46	contain	had	1012:1014	arg1	proteins					1003:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins	922:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins	922:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	46	contain	had	1012:1014	arg2	activities					1023:1032	varied activities	1016:1032	varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold	1016:1217	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	3	47	theme	Low	600:602	arg1	sKlotho					604:610	Low sKlotho	600:610	Low sKlotho in CKD	600:617	Low sKlotho in CKD is associated with disease progression, and sKlotho supplementation has emerged as a potential therapeutic strategy for managing CKD.					
32005658	0	48	theme	soluble	40:46	arg1	form					48:51	the soluble form	36:51	the soluble form of the antiaging protein Klotho	36:83	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	1	49	theme	similar	321:327	arg1	phenotype					300:308	a phenotype	298:308	a phenotype strikingly similar to that of chronic kidney disease (CKD)	298:367	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	6	50	from	Asn-126	1490:1496	arg1	at					1487:1488	at Asn-126	1487:1496	at Asn-126	1487:1496	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	6	51	from	sites	1467:1471	arg1	N					1414:1414	N	1414:1414	N	1414:1414	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	6	51	from	sites	1467:1471	arg1	diamine					1438:1444	N'-di-N-acetyllactose diamine	1416:1444	N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif	1416:1545	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	6	52	link	N-linked	1458:1465	arg1	sites					1467:1471	multiple N-linked sites	1449:1471	multiple N-linked sites	1449:1471	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	3	53	from	sKlotho	604:610	arg1	CKD					615:617	CKD	615:617	CKD	615:617	Low sKlotho in CKD is associated with disease progression, and sKlotho supplementation has emerged as a potential therapeutic strategy for managing CKD.					
32005658	0	54	contain	have	85:88	arg2	implications					102:113	therapeutic implications	90:113	therapeutic implications for managing kidney disease	90:141	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	0	54	contain	have	85:88	arg1	relationships					19:31	Structure-function relationships	0:31	Structure-function relationships of the soluble form of the antiaging protein Klotho	0:83	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	5	55	dep	activities	1023:1032	arg1	increased					1177:1185	increased	1177:1185	increased its co-receptor activity 3-fold	1177:1217	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	56	theme	CHO-sKlotho	1165:1175	arg1	treatment					1133:1141	in vivo Sialidase treatment	1115:1141	in vivo Sialidase treatment of heavily sialylated CHO-sKlotho	1115:1175	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	57	theme	human	955:959	arg1	HEK					979:981	HEK	979:981	HEK	979:981	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	57	theme	human	955:959	arg1	kidney					971:976	human embryonic kidney	955:976	human embryonic kidney (HEK)	955:982	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	4	58	theme	sKlotho	847:853	arg1	variants					855:862	sKlotho variants	847:862	sKlotho variants	847:862	Here, we explored the structure-function relationship and post-translational modifications of sKlotho variants to guide the future design of sKlotho-based therapeutics.					
32005658	8	59	theme	glutamate	1723:1731	arg1	residues					1733:1740	two catalytic glutamate residues	1709:1740	two catalytic glutamate residues conserved across glycosidases	1709:1770	Moreover, the introduction of two catalytic glutamate residues conserved across glycosidases into sKlotho enhanced its glucuronidase activity but decreased its FGF23 co-receptor activity, suggesting that these two functions might be structurally divergent.					
32005658	4	60	theme	structure-function	775:792	arg1	relationship					794:805	structure-function relationship	775:805	structure-function relationship	775:805	Here, we explored the structure-function relationship and post-translational modifications of sKlotho variants to guide the future design of sKlotho-based therapeutics.					
32005658	9	61	theme	rational	1977:1984	arg1	engineering					1986:1996	rational engineering	1977:1996	rational engineering of pharmacologically enhanced sKlotho therapeutics for managing kidney disease	1977:2075	These findings open up opportunities for rational engineering of pharmacologically enhanced sKlotho therapeutics for managing kidney disease.					
32005658	6	62	theme	unusual	1373:1379	arg1	structure					1390:1398	an unusual N-glycan structure	1370:1398	an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif	1370:1545	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	6	63	theme	GalNAc	1525:1530	arg1	motif					1541:1545	a putative GalNAc transfer motif	1514:1545	a putative GalNAc transfer motif	1514:1545	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	2	64	theme	humoral	556:562	arg1	factor					564:569	a humoral factor	554:569	a humoral factor that regulates renal health	554:597	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	64	theme	humoral	556:562	arg1	domain					486:491	its shed extracellular domain	463:491	its shed extracellular domain	463:491	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	5	65	theme	-derived	983:990	arg1	proteins					1003:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins	922:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins	922:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	7	66	theme	protein	1649:1655	arg1	folding					1657:1663	Klotho's protein folding	1640:1663	Klotho's protein folding	1640:1663	Site-directed mutagenesis and structural modeling analyses directly implicated N-glycans in Klotho's protein folding and function.					
32005658	9	67	theme	enhanced	2019:2026	arg1	therapeutics					2036:2047	pharmacologically enhanced sKlotho therapeutics	2001:2047	pharmacologically enhanced sKlotho therapeutics	2001:2047	These findings open up opportunities for rational engineering of pharmacologically enhanced sKlotho therapeutics for managing kidney disease.					
32005658	5	68	link	-derived	983:990	arg1	proteins					1003:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins	922:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins	922:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	4	69	theme	sKlotho-based	894:906	arg1	therapeutics					908:919	sKlotho-based therapeutics	894:919	sKlotho-based therapeutics	894:919	Here, we explored the structure-function relationship and post-translational modifications of sKlotho variants to guide the future design of sKlotho-based therapeutics.					
32005658	9	70	theme	therapeutics	2036:2047	arg1	engineering					1986:1996	rational engineering	1977:1996	rational engineering of pharmacologically enhanced sKlotho therapeutics for managing kidney disease	1977:2075	These findings open up opportunities for rational engineering of pharmacologically enhanced sKlotho therapeutics for managing kidney disease.					
32005658	5	71	gly	sialylated	1154:1163	arg1	CHO-sKlotho					1165:1175	heavily sialylated CHO-sKlotho	1146:1175	heavily sialylated CHO-sKlotho	1146:1175	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	72	theme	sKlotho	995:1001	arg1	proteins					1003:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins	922:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins	922:1010	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	0	73	theme	protein	70:76	arg1	Klotho					78:83	the antiaging protein Klotho	56:83	the antiaging protein Klotho	56:83	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	6	74	theme	N-glycan	1381:1388	arg1	structure					1390:1398	an unusual N-glycan structure	1370:1398	an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif	1370:1545	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	8	75	theme	FGF23	1839:1843	arg1	activity					1857:1864	its FGF23 co-receptor activity	1835:1864	its FGF23 co-receptor activity	1835:1864	Moreover, the introduction of two catalytic glutamate residues conserved across glycosidases into sKlotho enhanced its glucuronidase activity but decreased its FGF23 co-receptor activity, suggesting that these two functions might be structurally divergent.					
32005658	1	76	theme	antiaging	172:180	arg1	protein					191:197	The fortuitously discovered antiaging membrane protein αKlotho	144:205	The fortuitously discovered antiaging membrane protein αKlotho (Klotho)	144:214	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	1	76	theme	antiaging	172:180	arg1	Klotho					208:213	Klotho	208:213	Klotho	208:213	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	3	77	dep	potential	704:712	arg1	therapeutic					714:724	therapeutic	714:724	therapeutic	714:724	Low sKlotho in CKD is associated with disease progression, and sKlotho supplementation has emerged as a potential therapeutic strategy for managing CKD.					
32005658	5	78	theme	distinct	1095:1102	arg1	properties					1104:1113	distinct properties	1095:1113	distinct properties	1095:1113	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	7	79	theme	Site-directed	1548:1560	arg1	mutagenesis					1562:1572	Site-directed mutagenesis	1548:1572	Site-directed mutagenesis	1548:1572	Site-directed mutagenesis and structural modeling analyses directly implicated N-glycans in Klotho's protein folding and function.					
32005658	0	80	theme	managing	119:126	arg1	disease					135:141	managing kidney disease	119:141	managing kidney disease	119:141	Structure-function relationships of the soluble form of the antiaging protein Klotho have therapeutic implications for managing kidney disease.					
32005658	1	81	theme	kidney	348:353	arg1	CKD					364:366	CKD	364:366	CKD	364:366	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	1	81	theme	kidney	348:353	arg1	disease					355:361	chronic kidney disease	340:361	chronic kidney disease (CKD)	340:367	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	2	82	theme	shed	467:470	arg1	factor					564:569	a humoral factor	554:569	a humoral factor that regulates renal health	554:597	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	82	theme	shed	467:470	arg1	domain					486:491	its shed extracellular domain	463:491	its shed extracellular domain	463:491	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	82	theme	shed	467:470	arg1	Klotho					502:507	soluble Klotho	494:507	soluble Klotho (sKlotho)	494:517	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	6	83	theme	N-linked	1458:1465	arg1	sites					1467:1471	multiple N-linked sites	1449:1471	multiple N-linked sites	1449:1471	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	2	84	theme	growth	419:424	arg1	factor					426:431	fibroblast growth factor 23	408:434	fibroblast growth factor 23 (FGF23) signaling	408:452	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	84	theme	growth	419:424	arg1	FGF23					437:441	FGF23	437:441	FGF23	437:441	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	4	85	dep	relationship	794:805	arg1	the					771:773	the	771:773	the	771:773	Here, we explored the structure-function relationship and post-translational modifications of sKlotho variants to guide the future design of sKlotho-based therapeutics.					
32005658	1	86	theme	gene	278:281	arg1	deletion					255:262	deletion	255:262	deletion of the Klotho gene in mice	255:289	The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of the Klotho gene in mice causes a phenotype strikingly similar to that of chronic kidney disease (CKD).					
32005658	5	87	theme	hamster	930:936	arg1	CHO					945:947	CHO	945:947	CHO	945:947	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	87	theme	hamster	930:936	arg1	ovary					938:942	Chinese hamster ovary	922:942	Chinese hamster ovary	922:942	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	88	dep	in	1082:1083	arg1	vitro					1085:1089	vitro	1085:1089	vitro	1085:1089	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	89	gly	hyposialylated	1253:1266	arg1	HEK-sKlotho					1268:1278	hyposialylated HEK-sKlotho	1253:1278	hyposialylated HEK-sKlotho	1253:1278	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	2	90	contain	carrying	520:527	arg2	activity					541:548	glycosidase activity	529:548	glycosidase activity	529:548	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	90	contain	carrying	520:527	arg1	sKlotho					510:516	sKlotho	510:516	sKlotho	510:516	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	90	contain	carrying	520:527	arg1	domain					486:491	its shed extracellular domain	463:491	its shed extracellular domain	463:491	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	90	contain	carrying	520:527	arg1	Klotho					502:507	soluble Klotho	494:507	soluble Klotho (sKlotho)	494:517	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	6	91	theme	N'-di-N-acetyllactose	1416:1436	arg1	N					1414:1414	N	1414:1414	N	1414:1414	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	6	91	theme	N'-di-N-acetyllactose	1416:1436	arg1	diamine					1438:1444	N'-di-N-acetyllactose diamine	1416:1444	N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif	1416:1545	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	5	92	theme	sialylated	1154:1163	arg1	CHO-sKlotho					1165:1175	heavily sialylated CHO-sKlotho	1146:1175	heavily sialylated CHO-sKlotho	1146:1175	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	2	93	dep	functions	377:385	arg1	whereas					455:461	whereas	455:461	whereas	455:461	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	5	94	theme	in	1115:1116	arg1	treatment					1133:1141	in vivo Sialidase treatment	1115:1141	in vivo Sialidase treatment of heavily sialylated CHO-sKlotho	1115:1175	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	2	95	theme	soluble	494:500	arg1	sKlotho					510:516	sKlotho	510:516	sKlotho	510:516	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	95	theme	soluble	494:500	arg1	domain					486:491	its shed extracellular domain	463:491	its shed extracellular domain	463:491	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	2	95	theme	soluble	494:500	arg1	Klotho					502:507	soluble Klotho	494:507	soluble Klotho (sKlotho)	494:517	Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble Klotho (sKlotho), carrying glycosidase activity, is a humoral factor that regulates renal health.					
32005658	5	96	theme	Sialidase	1123:1131	arg1	treatment					1133:1141	in vivo Sialidase treatment	1115:1141	in vivo Sialidase treatment of heavily sialylated CHO-sKlotho	1115:1175	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	6	97	theme	at	1487:1488	arg1	Asn-126					1490:1496	at Asn-126	1487:1496	at Asn-126	1487:1496	MS and glycopeptide-mapping analyses revealed that HEK-sKlotho is uniquely modified with an unusual N-glycan structure consisting of N,N'-di-N-acetyllactose diamine at multiple N-linked sites, one of which at Asn-126 was adjacent to a putative GalNAc transfer motif.					
32005658	5	98	theme	embryonic	961:969	arg1	HEK					979:981	HEK	979:981	HEK	979:981	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	5	98	theme	embryonic	961:969	arg1	kidney					971:976	human embryonic kidney	955:976	human embryonic kidney (HEK)	955:982	Chinese hamster ovary (CHO)- and human embryonic kidney (HEK)-derived WT sKlotho proteins had varied activities in FGF23 co-receptor and β-glucuronidase assays in vitro and distinct properties in vivo Sialidase treatment of heavily sialylated CHO-sKlotho increased its co-receptor activity 3-fold, yet it remained less active than hyposialylated HEK-sKlotho.					
32005658	3	99	theme	disease	638:644	arg1	progression					646:656	disease progression	638:656	disease progression	638:656	Low sKlotho in CKD is associated with disease progression, and sKlotho supplementation has emerged as a potential therapeutic strategy for managing CKD.					
32743578	3	0	theme	bioinformatic	547:559	arg1	analyses					561:568	bioinformatic analyses	547:568	bioinformatic analyses of natural variants	547:588	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	1	1	theme	receptor	251:258	arg1	glycoprotein					265:276	the cell surface receptor ACE2 glycoprotein	234:276	the cell surface receptor ACE2 glycoprotein	234:276	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	1	2	theme	ACE2	260:263	arg1	glycoprotein					265:276	the cell surface receptor ACE2 glycoprotein	234:276	the cell surface receptor ACE2 glycoprotein	234:276	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	4	3	theme	polypeptide	810:820	arg1	epitopes					822:829	polypeptide epitopes	810:829	polypeptide epitopes	810:829	Our results highlight roles for glycans in sterically masking polypeptide epitopes and directly modulating Spike-ACE2 interactions.					
32743578	4	4	theme	Spike-ACE2	855:864	arg1	interactions					866:877	Spike-ACE2 interactions	855:877	Spike-ACE2 interactions	855:877	Our results highlight roles for glycans in sterically masking polypeptide epitopes and directly modulating Spike-ACE2 interactions.					
32743578	2	5	theme	glycomics-informed	322:339	arg1	glycoproteomics					341:355	glycomics-informed glycoproteomics	322:355	glycomics-informed glycoproteomics	322:355	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	5	6	from	impact	920:925	arg1	glycosylation					1047:1059	ACE2 receptor glycosylation	1033:1059	ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection	1033:1209	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	5	6	from	impact	920:925	arg1	glycosylation					970:982	Spike glycosylation	964:982	Spike glycosylation	964:982	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	1	7	gly	glycosylated	187:198	arg1	protein					215:221	its highly glycosylated trimeric Spike protein	176:221	its highly glycosylated trimeric Spike protein	176:221	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	3	8	theme	existing	599:606	arg1	3D-structures					608:620	existing 3D-structures	599:620	existing 3D-structures of both glycoproteins	599:642	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	5	9	dep	facilitate	1087:1096	arg1	taken					1067:1071	taken	1067:1071	taken together	1067:1080	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	5	10	theme	variants	1021:1028	arg1	impact					920:925	the impact	916:925	the impact of viral evolution and divergence on Spike glycosylation	916:982	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	5	10	theme	variants	1021:1028	arg1	influence					1000:1008	the influence	996:1008	the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection	996:1209	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	3	11	gly	glycoproteins	630:642	arg1	glycoproteins					630:642	both glycoproteins	625:642	both glycoproteins	625:642	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	3	12	theme	variants	581:588	arg1	analyses					561:568	bioinformatic analyses	547:568	bioinformatic analyses of natural variants	547:588	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	2	13	theme	recombinant	429:439	arg1	trimer					441:446	a recombinant trimer	427:446	a recombinant trimer Spike mimetic immunogen	427:470	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	2	14	theme	Spike	448:452	arg1	immunogen					462:470	a recombinant trimer Spike mimetic immunogen	427:470	a recombinant trimer Spike mimetic immunogen	427:470	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	3	15	dep	one	735:737	arg1	another					739:745	another	739:745	another	739:745	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	2	16	theme	trimer	441:446	arg1	immunogen					462:470	a recombinant trimer Spike mimetic immunogen	427:470	a recombinant trimer Spike mimetic immunogen	427:470	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	3	17	theme	natural	573:579	arg1	variants					581:588	natural variants	573:588	natural variants	573:588	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	1	18	theme	current	90:96	arg1	pandemic					107:114	The current COVID-19 pandemic	86:114	The current COVID-19 pandemic	86:114	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	1	19	theme	glycosylated	187:198	arg1	protein					215:221	its highly glycosylated trimeric Spike protein	176:221	its highly glycosylated trimeric Spike protein	176:221	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	0	20	theme	Virus-Receptor	0:13	arg1	Interactions					15:26	Virus-Receptor Interactions	0:26	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor	0:83	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32743578	5	21	theme	therapeutic	1161:1171	arg1	strategies					1173:1182	therapeutic strategies	1161:1182	therapeutic strategies	1161:1182	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	5	22	from	influence	1000:1008	arg1	glycosylation					1047:1059	ACE2 receptor glycosylation	1033:1059	ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection	1033:1209	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	5	22	from	influence	1000:1008	arg1	glycosylation					970:982	Spike glycosylation	964:982	Spike glycosylation	964:982	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	1	23	theme	COVID-19	98:105	arg1	pandemic					107:114	The current COVID-19 pandemic	86:114	The current COVID-19 pandemic	86:114	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	1	24	theme	trimeric	200:207	arg1	protein					215:221	its highly glycosylated trimeric Spike protein	176:221	its highly glycosylated trimeric Spike protein	176:221	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	0	25	theme	Glycosylated	31:42	arg1	Spike					55:59	Glycosylated SARS-CoV-2 Spike	31:59	Glycosylated SARS-CoV-2 Spike	31:59	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32743578	1	26	theme	host	293:296	arg1	entry					303:307	host cell entry	293:307	host cell entry	293:307	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	1	27	gly	glycoprotein	265:276	arg1	glycoprotein					265:276	the cell surface receptor ACE2 glycoprotein	234:276	the cell surface receptor ACE2 glycoprotein	234:276	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	5	28	theme	receptor	1038:1045	arg1	glycosylation					1047:1059	ACE2 receptor glycosylation	1033:1059	ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection	1033:1209	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	1	29	theme	Spike	209:213	arg1	protein					215:221	its highly glycosylated trimeric Spike protein	176:221	its highly glycosylated trimeric Spike protein	176:221	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	3	30	theme	glycoproteins	630:642	arg1	3D-structures					608:620	existing 3D-structures	599:620	existing 3D-structures of both glycoproteins	599:642	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	1	31	theme	cell	298:301	arg1	entry					303:307	host cell entry	293:307	host cell entry	293:307	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	5	32	theme	ACE2	1033:1036	arg1	glycosylation					1047:1059	ACE2 receptor glycosylation	1033:1059	ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection	1033:1209	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	2	33	theme	ACE2	507:510	arg1	version					490:496	a soluble version	480:496	a soluble version of human ACE2	480:510	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	5	34	theme	evolution	936:944	arg1	impact					920:925	the impact	916:925	the impact of viral evolution and divergence on Spike glycosylation	916:982	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	5	34	theme	evolution	936:944	arg1	influence					1000:1008	the influence	996:1008	the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection	996:1209	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	0	35	theme	Spike	55:59	arg1	Interactions					15:26	Virus-Receptor Interactions	0:26	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor	0:83	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32743578	2	36	gly	microheterogeneity	387:404	arg1	glycosylation					409:421	glycosylation	409:421	glycosylation	409:421	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	3	37	theme	molecular	656:664	arg1	simulations					675:685	molecular dynamics simulations	656:685	molecular dynamics simulations of each glycoprotein	656:706	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	0	38	theme	SARS-CoV-2	44:53	arg1	Spike					55:59	Glycosylated SARS-CoV-2 Spike	31:59	Glycosylated SARS-CoV-2 Spike	31:59	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32743578	2	39	theme	soluble	482:488	arg1	version					490:496	a soluble version	480:496	a soluble version of human ACE2	480:510	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	2	40	theme	mimetic	454:460	arg1	immunogen					462:470	a recombinant trimer Spike mimetic immunogen	427:470	a recombinant trimer Spike mimetic immunogen	427:470	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	0	41	theme	Human	65:69	arg1	Receptor					76:83	Human ACE2 Receptor	65:83	Human ACE2 Receptor	65:83	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32743578	5	42	theme	viral	1195:1199	arg1	infection					1201:1209	viral infection	1195:1209	viral infection	1195:1209	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	2	43	theme	site-specific	373:385	arg1	microheterogeneity					387:404	site-specific microheterogeneity	373:404	site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2	373:510	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	2	44	theme	human	501:505	arg1	ACE2					507:510	human ACE2	501:510	human ACE2	501:510	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	5	45	theme	antibody	1126:1133	arg1	neutralization					1135:1148	antibody neutralization	1126:1148	antibody neutralization	1126:1148	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	3	46	gly	glycoprotein	695:706	arg1	glycoprotein					695:706	each glycoprotein	690:706	each glycoprotein	690:706	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	3	47	theme	dynamics	666:673	arg1	simulations					675:685	molecular dynamics simulations	656:685	molecular dynamics simulations of each glycoprotein	656:706	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	5	48	theme	natural	1013:1019	arg1	variants					1021:1028	natural variants	1013:1028	natural variants	1013:1028	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	3	49	theme	glycoprotein	695:706	arg1	simulations					675:685	molecular dynamics simulations	656:685	molecular dynamics simulations of each glycoprotein	656:706	We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another.					
32743578	1	50	theme	SARS-CoV-2	133:142	arg1	betacoronavirus					144:158	the SARS-CoV-2 betacoronavirus	129:158	the SARS-CoV-2 betacoronavirus	129:158	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	2	51	used	utilized	313:320	arg2	We					310:311	We	310:311	We	310:311	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	0	52	theme	Receptor	76:83	arg1	Interactions					15:26	Virus-Receptor Interactions	0:26	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor	0:83	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32743578	5	53	theme	divergence	950:959	arg1	impact					920:925	the impact	916:925	the impact of viral evolution and divergence on Spike glycosylation	916:982	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	5	53	theme	divergence	950:959	arg1	influence					1000:1008	the influence	996:1008	the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection	996:1209	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	5	54	theme	viral	930:934	arg1	evolution					936:944	viral evolution	930:944	viral evolution	930:944	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	5	55	theme	Spike	964:968	arg1	glycosylation					970:982	Spike glycosylation	964:982	Spike glycosylation	964:982	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	1	56	theme	cell	238:241	arg1	glycoprotein					265:276	the cell surface receptor ACE2 glycoprotein	234:276	the cell surface receptor ACE2 glycoprotein	234:276	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
32743578	0	57	theme	ACE2	71:74	arg1	Receptor					76:83	Human ACE2 Receptor	65:83	Human ACE2 Receptor	65:83	Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor.					
32743578	5	58	dep	design	1108:1113	arg1	achieve					1118:1124	achieve	1118:1124	to achieve antibody neutralization	1115:1148	Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.					
32743578	2	59	theme	glycosylation	409:421	arg1	microheterogeneity					387:404	site-specific microheterogeneity	373:404	site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2	373:510	We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2.					
32743578	1	60	theme	surface	243:249	arg1	glycoprotein					265:276	the cell surface receptor ACE2 glycoprotein	234:276	the cell surface receptor ACE2 glycoprotein	234:276	The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry.					
33053983	0	0	theme	technology	71:80	arg1	method					98:103	DSA-FACE technology based-glycomics method	62:103	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method	0:103	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method].					
33053983	5	1	theme	N-linked	695:702	arg1	glycans					704:710	N-linked glycans	695:710	N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	695:800	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	0	2	theme	DSA-FACE	62:69	arg1	method					98:103	DSA-FACE technology based-glycomics method	62:103	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method	0:103	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method].					
33053983	4	3	theme	past	469:472	arg1	decade					474:479	the past decade	465:479	the past decade	465:479	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	1	4	theme	serious	152:158	arg1	problem					167:173	a serious health problem worldwide	150:183	a serious health problem worldwide	150:183	Chronic liver disease has gradually become a serious health problem worldwide.					
33053983	5	5	theme	chronic	900:906	arg1	diseases					914:921	various chronic liver diseases	892:921	various chronic liver diseases	892:921	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	6	theme	diagnostic	852:861	arg1	models					863:868	high sensitivity and specificity diagnostic models	819:868	high sensitivity and specificity diagnostic models (GlycoHepatoTest)	819:886	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	6	theme	diagnostic	852:861	arg1	strategy					939:946	a new strategy	933:946	a new strategy for non-invasive diagnosis of liver diseases	933:991	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	6	theme	diagnostic	852:861	arg1	GlycoHepatoTest					871:885	GlycoHepatoTest	871:885	GlycoHepatoTest	871:885	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	0	7	theme	based-glycomics	82:96	arg1	method					98:103	DSA-FACE technology based-glycomics method	62:103	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method	0:103	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method].					
33053983	5	8	theme	various	892:898	arg1	diseases					914:921	various chronic liver diseases	892:921	various chronic liver diseases	892:921	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	9	dep	sequencing	720:729	arg1	DSA-FACE					792:799	DSA-FACE	792:799	DSA-FACE	792:799	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	9	dep	sequencing	720:729	arg1	Electrophoresis					775:789	equipment-Fluorophore Assisted Carbohydrate Electrophoresis	731:789	DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:800	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	10	theme	Assisted	753:760	arg1	DSA-FACE					792:799	DSA-FACE	792:799	DSA-FACE	792:799	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	10	theme	Assisted	753:760	arg1	Electrophoresis					775:789	equipment-Fluorophore Assisted Carbohydrate Electrophoresis	731:789	DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:800	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	3	11	theme	rapid	384:388	arg1	method					413:418	an economical and rapid non-invasive detection method	366:418	an economical and rapid non-invasive detection method that can be used in clinic for diagnosis	366:459	Therefore, there is an urgent need for an economical and rapid non-invasive detection method that can be used in clinic for diagnosis.					
33053983	1	12	theme	health	160:165	arg1	problem					167:173	a serious health problem worldwide	150:183	a serious health problem worldwide	150:183	Chronic liver disease has gradually become a serious health problem worldwide.					
33053983	5	13	theme	same	684:687	arg1	time					689:692	the same time	680:692	the same time	680:692	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	14	theme	Carbohydrate	762:773	arg1	DSA-FACE					792:799	DSA-FACE	792:799	DSA-FACE	792:799	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	14	theme	Carbohydrate	762:773	arg1	Electrophoresis					775:789	equipment-Fluorophore Assisted Carbohydrate Electrophoresis	731:789	DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:800	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	15	theme	high	819:822	arg1	sensitivity					824:834	high sensitivity	819:834	high sensitivity	819:834	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	0	16	theme	new	3:5	arg1	strategy					7:14	[A new strategy	0:14	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method	0:103	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method].					
33053983	4	17	from	concept	543:549	arg1	structure					585:593	serum proteoglycans structure	565:593	serum proteoglycans structure	565:593	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	4	18	from	changes	554:560	arg1	structure					585:593	serum proteoglycans structure	565:593	serum proteoglycans structure	565:593	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	5	19	link	N-linked	695:702	arg1	glycans					704:710	N-linked glycans	695:710	N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	695:800	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	1	20	theme	worldwide	175:183	arg1	problem					167:173	a serious health problem worldwide	150:183	a serious health problem worldwide	150:183	Chronic liver disease has gradually become a serious health problem worldwide.					
33053983	0	21	theme	[A	0:1	arg1	strategy					7:14	[A new strategy	0:14	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method	0:103	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method].					
33053983	5	22	theme	liver	908:912	arg1	diseases					914:921	various chronic liver diseases	892:921	various chronic liver diseases	892:921	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	3	23	theme	non-invasive	390:401	arg1	method					413:418	an economical and rapid non-invasive detection method	366:418	an economical and rapid non-invasive detection method that can be used in clinic for diagnosis	366:459	Therefore, there is an urgent need for an economical and rapid non-invasive detection method that can be used in clinic for diagnosis.					
33053983	5	24	theme	non-invasive	952:963	arg1	diagnosis					965:973	non-invasive diagnosis	952:973	non-invasive diagnosis of liver diseases	952:991	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	3	25	used	used	432:435	arg2	method					413:418	an economical and rapid non-invasive detection method	366:418	an economical and rapid non-invasive detection method that can be used in clinic for diagnosis	366:459	Therefore, there is an urgent need for an economical and rapid non-invasive detection method that can be used in clinic for diagnosis.					
33053983	4	26	from	structure	585:593	arg1	indication					649:658	an indication	646:658	an indication of liver injury	646:674	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	4	26	from	structure	585:593	arg1	concept					543:549	the concept	539:549	the concept of changes in serum proteoglycans structure	539:593	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	4	27	theme	proteoglycans	571:583	arg1	structure					585:593	serum proteoglycans structure	565:593	serum proteoglycans structure	565:593	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	4	28	theme	research	517:524	arg1	hotspot					526:532	a research hotspot	515:532	a research hotspot	515:532	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	4	29	theme	serum	565:569	arg1	structure					585:593	serum proteoglycans structure	565:593	serum proteoglycans structure	565:593	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	3	30	theme	economical	369:378	arg1	method					413:418	an economical and rapid non-invasive detection method	366:418	an economical and rapid non-invasive detection method that can be used in clinic for diagnosis	366:459	Therefore, there is an urgent need for an economical and rapid non-invasive detection method that can be used in clinic for diagnosis.					
33053983	2	31	theme	severe	305:310	arg1	complications					312:324	severe complications	305:324	severe complications	305:324	Liver biopsy is the current gold standard to assess liver lesions; however, it is an invasive procedure that may cause severe complications.					
33053983	0	32	theme	non-invasive	20:31	arg1	diagnosis					33:41	non-invasive diagnosis	20:41	non-invasive diagnosis of liver diseases	20:59	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method].					
33053983	5	33	theme	DNA	716:718	arg1	sequencing					720:729	DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:800	DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:800	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	34	theme	liver	978:982	arg1	diseases					984:991	liver diseases	978:991	liver diseases	978:991	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	0	35	theme	liver	46:50	arg1	diseases					52:59	liver diseases	46:59	liver diseases	46:59	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method].					
33053983	5	36	theme	sensitivity	824:834	arg1	models					863:868	high sensitivity and specificity diagnostic models	819:868	high sensitivity and specificity diagnostic models (GlycoHepatoTest)	819:886	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	36	theme	sensitivity	824:834	arg1	strategy					939:946	a new strategy	933:946	a new strategy for non-invasive diagnosis of liver diseases	933:991	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	36	theme	sensitivity	824:834	arg1	GlycoHepatoTest					871:885	GlycoHepatoTest	871:885	GlycoHepatoTest	871:885	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	3	37	theme	detection	403:411	arg1	method					413:418	an economical and rapid non-invasive detection method	366:418	an economical and rapid non-invasive detection method that can be used in clinic for diagnosis	366:459	Therefore, there is an urgent need for an economical and rapid non-invasive detection method that can be used in clinic for diagnosis.					
33053983	5	38	theme	diseases	984:991	arg1	diagnosis					965:973	non-invasive diagnosis	952:973	non-invasive diagnosis of liver diseases	952:991	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	1	39	theme	Chronic	107:113	arg1	disease					121:127	Chronic liver disease	107:127	Chronic liver disease	107:127	Chronic liver disease has gradually become a serious health problem worldwide.					
33053983	5	40	theme	equipment-Fluorophore	731:751	arg1	DSA-FACE					792:799	DSA-FACE	792:799	DSA-FACE	792:799	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	40	theme	equipment-Fluorophore	731:751	arg1	Electrophoresis					775:789	equipment-Fluorophore Assisted Carbohydrate Electrophoresis	731:789	DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE)	716:800	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	1	41	theme	liver	115:119	arg1	disease					121:127	Chronic liver disease	107:127	Chronic liver disease	107:127	Chronic liver disease has gradually become a serious health problem worldwide.					
33053983	4	42	theme	protein	482:488	arg1	glycosylation					490:502	protein glycosylation	482:502	protein glycosylation	482:502	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	4	43	theme	many	626:629	arg1	researchers					631:641	many researchers	626:641	many researchers	626:641	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	4	44	theme	injury	669:674	arg1	concept					543:549	the concept	539:549	the concept of changes in serum proteoglycans structure	539:593	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	4	44	theme	injury	669:674	arg1	indication					649:658	an indication	646:658	an indication of liver injury	646:674	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	2	45	theme	invasive	271:278	arg1	it					262:263	it	262:263	it	262:263	Liver biopsy is the current gold standard to assess liver lesions; however, it is an invasive procedure that may cause severe complications.					
33053983	2	45	theme	invasive	271:278	arg1	procedure					280:288	an invasive procedure	268:288	an invasive procedure that may cause severe complications	268:324	Liver biopsy is the current gold standard to assess liver lesions; however, it is an invasive procedure that may cause severe complications.					
33053983	5	46	theme	new	935:937	arg1	models					863:868	high sensitivity and specificity diagnostic models	819:868	high sensitivity and specificity diagnostic models (GlycoHepatoTest)	819:886	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	46	theme	new	935:937	arg1	strategy					939:946	a new strategy	933:946	a new strategy for non-invasive diagnosis of liver diseases	933:991	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	0	47	dep	strategy	7:14	arg1	method					98:103	DSA-FACE technology based-glycomics method	62:103	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method	0:103	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method].					
33053983	4	48	theme	liver	663:667	arg1	injury					669:674	liver injury	663:674	liver injury	663:674	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	2	49	theme	Liver	186:190	arg1	standard					219:226	standard	219:226	standard	219:226	Liver biopsy is the current gold standard to assess liver lesions; however, it is an invasive procedure that may cause severe complications.					
33053983	2	49	theme	Liver	186:190	arg1	biopsy					192:197	Liver biopsy	186:197	Liver biopsy	186:197	Liver biopsy is the current gold standard to assess liver lesions; however, it is an invasive procedure that may cause severe complications.					
33053983	2	50	theme	liver	238:242	arg1	lesions					244:250	liver lesions	238:250	liver lesions	238:250	Liver biopsy is the current gold standard to assess liver lesions; however, it is an invasive procedure that may cause severe complications.					
33053983	5	51	theme	specificity	840:850	arg1	models					863:868	high sensitivity and specificity diagnostic models	819:868	high sensitivity and specificity diagnostic models (GlycoHepatoTest)	819:886	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	51	theme	specificity	840:850	arg1	strategy					939:946	a new strategy	933:946	a new strategy for non-invasive diagnosis of liver diseases	933:991	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	5	51	theme	specificity	840:850	arg1	GlycoHepatoTest					871:885	GlycoHepatoTest	871:885	GlycoHepatoTest	871:885	At the same time, N-linked glycans via DNA sequencing equipment-Fluorophore Assisted Carbohydrate Electrophoresis (DSA-FACE) has also brought high sensitivity and specificity diagnostic models (GlycoHepatoTest) for various chronic liver diseases, which is a new strategy for non-invasive diagnosis of liver diseases.					
33053983	0	52	theme	diseases	52:59	arg1	diagnosis					33:41	non-invasive diagnosis	20:41	non-invasive diagnosis of liver diseases	20:59	[A new strategy for non-invasive diagnosis of liver diseases: DSA-FACE technology based-glycomics method].					
33053983	3	53	theme	urgent	350:355	arg1	need					357:360	an urgent need	347:360	an urgent need for an economical and rapid non-invasive detection method that can be used in clinic for diagnosis	347:459	Therefore, there is an urgent need for an economical and rapid non-invasive detection method that can be used in clinic for diagnosis.					
33053983	4	54	theme	changes	554:560	arg1	indication					649:658	an indication	646:658	an indication of liver injury	646:674	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
33053983	4	54	theme	changes	554:560	arg1	concept					543:549	the concept	539:549	the concept of changes in serum proteoglycans structure	539:593	In the past decade, protein glycosylation has become a research hotspot, and the concept of changes in serum proteoglycans structure has gradually been accepted by many researchers as an indication of liver injury.					
32914709	7	0	theme	sialylation	1516:1526	arg1	level					1507:1511	the level	1503:1511	the level of sialylation	1503:1526	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	5	1	theme	resistance	902:911	arg1	phenotype					913:921	the multidrug resistance phenotype	888:921	the multidrug resistance phenotype	888:921	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	7	2	theme	multidrug	1337:1345	arg1	phenotype					1358:1366	the induced cell multidrug resistance phenotype	1320:1366	the induced cell multidrug resistance phenotype	1320:1366	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	1	3	theme	multidrug	176:184	arg1	phenotype					197:205	the multidrug resistance phenotype	172:205	the multidrug resistance phenotype	172:205	The development of the multidrug resistance phenotype is one of the major challenges faced in the treatment of cancer.					
32914709	7	4	from	increase	1491:1498	arg1	level					1507:1511	the level	1503:1511	the level of sialylation	1503:1526	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	7	4	from	increase	1491:1498	arg1	number					1539:1544	the number	1535:1544	the number of branched glycans	1535:1564	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	1	5	theme	cancer	264:269	arg1	treatment					251:259	the treatment	247:259	the treatment of cancer	247:269	The development of the multidrug resistance phenotype is one of the major challenges faced in the treatment of cancer.					
32914709	0	6	theme	prostate	121:128	arg1	PC-3					147:150	the human prostate cancer cell line PC-3	111:150	the human prostate cancer cell line PC-3	111:150	Resistance to paclitaxel induces glycophenotype changes and mesenchymal-to-epithelial transition activation in the human prostate cancer cell line PC-3.					
32914709	3	7	theme	other	712:716	arg1	organs					718:723	other organs	712:723	other organs	712:723	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	6	8	from	phenotype	1180:1188	arg1	line					1232:1235	the PC-3 human prostate adenocarcinoma line	1193:1235	the PC-3 human prostate adenocarcinoma line	1193:1235	Herein, we induced the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line through the continuous treatment with the drug paclitaxel.					
32914709	4	9	theme	atypical	768:775	arg1	glycosylations					777:790	atypical glycosylations	768:790	atypical glycosylations	768:790	In addition, it is known that atypical glycosylations are characteristic of tumor cells, being used as biomarkers.					
32914709	6	10	theme	PC-3	1197:1200	arg1	line					1232:1235	the PC-3 human prostate adenocarcinoma line	1193:1235	the PC-3 human prostate adenocarcinoma line	1193:1235	Herein, we induced the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line through the continuous treatment with the drug paclitaxel.					
32914709	5	11	dep	chemoresistance	1068:1082	arg1	processes					1125:1133	processes	1125:1133	processes	1125:1133	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	0	12	theme	human	115:119	arg1	PC-3					147:150	the human prostate cancer cell line PC-3	111:150	the human prostate cancer cell line PC-3	111:150	Resistance to paclitaxel induces glycophenotype changes and mesenchymal-to-epithelial transition activation in the human prostate cancer cell line PC-3.					
32914709	7	13	dep	acquired	1372:1379	arg1	1					1369:1369	1	1369:1369	1	1369:1369	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	3	14	theme	tumor	581:585	arg1	cell					587:590	the tumor cell	577:590	the tumor cell	577:590	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	6	15	theme	resistance	1169:1178	arg1	phenotype					1180:1188	the multidrug resistance phenotype	1155:1188	the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line	1155:1235	Herein, we induced the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line through the continuous treatment with the drug paclitaxel.					
32914709	0	16	theme	cell	137:140	arg1	PC-3					147:150	the human prostate cancer cell line PC-3	111:150	the human prostate cancer cell line PC-3	111:150	Resistance to paclitaxel induces glycophenotype changes and mesenchymal-to-epithelial transition activation in the human prostate cancer cell line PC-3.					
32914709	7	17	theme	resistance	1347:1356	arg1	phenotype					1358:1366	the induced cell multidrug resistance phenotype	1320:1366	the induced cell multidrug resistance phenotype	1320:1366	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	2	18	theme	resistance	286:295	arg1	phenotype					297:305	The multidrug resistance phenotype	272:305	The multidrug resistance phenotype	272:305	The multidrug resistance phenotype is characterized by cross-resistance to drugs with different chemical structures and mechanisms of action.					
32914709	7	19	theme	epithelial	1405:1414	arg1	phenotypes					1432:1441	epithelial and mesenchymal phenotypes	1405:1441	epithelial and mesenchymal phenotypes	1405:1441	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	1	20	theme	resistance	186:195	arg1	phenotype					197:205	the multidrug resistance phenotype	172:205	the multidrug resistance phenotype	172:205	The development of the multidrug resistance phenotype is one of the major challenges faced in the treatment of cancer.					
32914709	0	21	theme	cancer	130:135	arg1	PC-3					147:150	the human prostate cancer cell line PC-3	111:150	the human prostate cancer cell line PC-3	111:150	Resistance to paclitaxel induces glycophenotype changes and mesenchymal-to-epithelial transition activation in the human prostate cancer cell line PC-3.					
32914709	3	22	theme	tissues	729:735	arg1	invasion					700:707	the invasion	696:707	the invasion of other organs and tissues	696:735	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	7	23	dep	modified	1451:1458	arg1	2					1448:1448	2	1448:1448	2	1448:1448	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	2	24	theme	multidrug	276:284	arg1	phenotype					297:305	The multidrug resistance phenotype	272:305	The multidrug resistance phenotype	272:305	The multidrug resistance phenotype is characterized by cross-resistance to drugs with different chemical structures and mechanisms of action.					
32914709	1	25	theme	phenotype	197:205	arg1	one					210:212	one	210:212	one	210:212	The development of the multidrug resistance phenotype is one of the major challenges faced in the treatment of cancer.					
32914709	1	25	theme	phenotype	197:205	arg1	development					157:167	The development	153:167	The development of the multidrug resistance phenotype	153:205	The development of the multidrug resistance phenotype is one of the major challenges faced in the treatment of cancer.					
32914709	1	25	theme	phenotype	197:205	arg1	challenges					227:236	the major challenges	217:236	the major challenges faced in the treatment of cancer	217:269	The development of the multidrug resistance phenotype is one of the major challenges faced in the treatment of cancer.					
32914709	6	26	theme	adenocarcinoma	1217:1230	arg1	line					1232:1235	the PC-3 human prostate adenocarcinoma line	1193:1235	the PC-3 human prostate adenocarcinoma line	1193:1235	Herein, we induced the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line through the continuous treatment with the drug paclitaxel.					
32914709	5	27	theme	epithelial-to-mesenchymal	945:969	arg1	transition					971:980	epithelial-to-mesenchymal transition	945:980	epithelial-to-mesenchymal transition	945:980	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	3	28	from	step	653:656	arg1	progression					667:677	tumor progression	661:677	tumor progression	661:677	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	3	28	from	step	653:656	arg1	promoting					686:694	promoting	686:694	promoting the invasion of other organs and tissues	686:735	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	7	29	theme	branched	1549:1556	arg1	glycans					1558:1564	branched glycans	1549:1564	branched glycans	1549:1564	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	5	30	theme	transition	971:980	arg1	activation					931:940	the activation	927:940	the activation of epithelial-to-mesenchymal transition	927:980	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	5	30	theme	transition	971:980	arg1	acquisition					873:883	the acquisition	869:883	the acquisition of the multidrug resistance phenotype	869:921	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	2	31	theme	action	406:411	arg1	mechanisms					392:401	mechanisms	392:401	mechanisms of action	392:411	The multidrug resistance phenotype is characterized by cross-resistance to drugs with different chemical structures and mechanisms of action.					
32914709	2	31	theme	action	406:411	arg1	structures					377:386	different chemical structures	358:386	different chemical structures	358:386	The multidrug resistance phenotype is characterized by cross-resistance to drugs with different chemical structures and mechanisms of action.					
32914709	4	32	theme	cells	820:824	arg1	characteristic					796:809	characteristic	796:809	characteristic	796:809	In addition, it is known that atypical glycosylations are characteristic of tumor cells, being used as biomarkers.					
32914709	6	33	theme	human	1202:1206	arg1	line					1232:1235	the PC-3 human prostate adenocarcinoma line	1193:1235	the PC-3 human prostate adenocarcinoma line	1193:1235	Herein, we induced the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line through the continuous treatment with the drug paclitaxel.					
32914709	6	34	theme	prostate	1208:1215	arg1	line					1232:1235	the PC-3 human prostate adenocarcinoma line	1193:1235	the PC-3 human prostate adenocarcinoma line	1193:1235	Herein, we induced the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line through the continuous treatment with the drug paclitaxel.					
32914709	8	35	theme	poor	1614:1617	arg1	prognosis					1619:1627	poor prognosis	1614:1627	poor prognosis	1614:1627	Both mechanisms are described as indicators of poor prognosis.					
32914709	0	36	theme	line	142:145	arg1	PC-3					147:150	the human prostate cancer cell line PC-3	111:150	the human prostate cancer cell line PC-3	111:150	Resistance to paclitaxel induces glycophenotype changes and mesenchymal-to-epithelial transition activation in the human prostate cancer cell line PC-3.					
32914709	3	37	theme	epithelial-to-mesenchymal	525:549	arg1	process					562:568	the epithelial-to-mesenchymal transition process	521:568	the epithelial-to-mesenchymal transition process	521:568	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	6	38	theme	multidrug	1159:1167	arg1	phenotype					1180:1188	the multidrug resistance phenotype	1155:1188	the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line	1155:1235	Herein, we induced the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line through the continuous treatment with the drug paclitaxel.					
32914709	2	39	with	drugs	347:351	arg1	mechanisms					392:401	mechanisms	392:401	mechanisms of action	392:411	The multidrug resistance phenotype is characterized by cross-resistance to drugs with different chemical structures and mechanisms of action.					
32914709	2	39	with	drugs	347:351	arg1	structures					377:386	different chemical structures	358:386	different chemical structures	358:386	The multidrug resistance phenotype is characterized by cross-resistance to drugs with different chemical structures and mechanisms of action.					
32914709	3	40	theme	invasive	619:626	arg1	phenotype					628:636	a more mobile and invasive phenotype	601:636	a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues	601:735	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	3	41	dep	phenotype	628:636	arg1	step					653:656	a fundamental step	639:656	a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues	601:735	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	1	42	theme	major	221:225	arg1	challenges					227:236	the major challenges	217:236	the major challenges faced in the treatment of cancer	217:269	The development of the multidrug resistance phenotype is one of the major challenges faced in the treatment of cancer.					
32914709	0	43	theme	glycophenotype	33:46	arg1	changes					48:54	glycophenotype changes	33:54	glycophenotype changes	33:54	Resistance to paclitaxel induces glycophenotype changes and mesenchymal-to-epithelial transition activation in the human prostate cancer cell line PC-3.					
32914709	6	44	with	treatment	1260:1268	arg1	paclitaxel					1284:1293	the drug paclitaxel	1275:1293	the drug paclitaxel	1275:1293	Herein, we induced the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line through the continuous treatment with the drug paclitaxel.					
32914709	5	45	used	used	1043:1046	arg2	glycophenotype					1014:1027	the cell glycophenotype	1005:1027	the cell glycophenotype	1005:1027	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	5	45	used	used	1043:1046	arg2	glycomarkers					1051:1062	glycomarkers	1051:1062	glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes	1051:1133	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	3	46	from	resistance	468:477	arg1	cancer					482:487	cancer	482:487	cancer	482:487	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	1	47	theme	challenges	227:236	arg1	one					210:212	one	210:212	one	210:212	The development of the multidrug resistance phenotype is one of the major challenges faced in the treatment of cancer.					
32914709	1	47	theme	challenges	227:236	arg1	development					157:167	The development	153:167	The development of the multidrug resistance phenotype	153:205	The development of the multidrug resistance phenotype is one of the major challenges faced in the treatment of cancer.					
32914709	1	47	theme	challenges	227:236	arg1	challenges					227:236	the major challenges	217:236	the major challenges faced in the treatment of cancer	217:269	The development of the multidrug resistance phenotype is one of the major challenges faced in the treatment of cancer.					
32914709	0	48	theme	mesenchymal-to-epithelial	60:84	arg1	activation					97:106	mesenchymal-to-epithelial transition activation	60:106	mesenchymal-to-epithelial transition activation	60:106	Resistance to paclitaxel induces glycophenotype changes and mesenchymal-to-epithelial transition activation in the human prostate cancer cell line PC-3.					
32914709	8	49	theme	prognosis	1619:1627	arg1	mechanisms					1572:1581	Both mechanisms	1567:1581	Both mechanisms	1567:1581	Both mechanisms are described as indicators of poor prognosis.					
32914709	8	49	theme	prognosis	1619:1627	arg1	indicators					1600:1609	indicators	1600:1609	indicators of poor prognosis	1600:1627	Both mechanisms are described as indicators of poor prognosis.					
32914709	7	50	theme	mesenchymal	1420:1430	arg1	phenotypes					1432:1441	epithelial and mesenchymal phenotypes	1405:1441	epithelial and mesenchymal phenotypes	1405:1441	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	3	51	theme	tumor	661:665	arg1	progression					667:677	tumor progression	661:677	tumor progression	661:677	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	3	52	theme	mobile	608:613	arg1	phenotype					628:636	a more mobile and invasive phenotype	601:636	a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues	601:735	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	2	53	theme	chemical	368:375	arg1	structures					377:386	different chemical structures	358:386	different chemical structures	358:386	The multidrug resistance phenotype is characterized by cross-resistance to drugs with different chemical structures and mechanisms of action.					
32914709	7	54	theme	mixed	1383:1387	arg1	profile					1389:1395	a mixed profile	1381:1395	a mixed profile between epithelial and mesenchymal phenotypes	1381:1441	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	7	55	theme	glycans	1558:1564	arg1	number					1539:1544	the number	1535:1544	the number of branched glycans	1535:1564	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	3	56	from	cancer	482:487	arg1	acquisition					453:463	the acquisition	449:463	the acquisition of resistance in cancer	449:487	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	2	57	theme	different	358:366	arg1	structures					377:386	different chemical structures	358:386	different chemical structures	358:386	The multidrug resistance phenotype is characterized by cross-resistance to drugs with different chemical structures and mechanisms of action.					
32914709	7	58	theme	induced	1324:1330	arg1	phenotype					1358:1366	the induced cell multidrug resistance phenotype	1320:1366	the induced cell multidrug resistance phenotype	1320:1366	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	6	59	theme	drug	1279:1282	arg1	paclitaxel					1284:1293	the drug paclitaxel	1275:1293	the drug paclitaxel	1275:1293	Herein, we induced the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line through the continuous treatment with the drug paclitaxel.					
32914709	3	60	theme	fundamental	641:651	arg1	step					653:656	a fundamental step	639:656	a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues	601:735	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	5	61	theme	phenotype	913:921	arg1	activation					931:940	the activation	927:940	the activation of epithelial-to-mesenchymal transition	927:980	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	5	61	theme	phenotype	913:921	arg1	acquisition					873:883	the acquisition	869:883	the acquisition of the multidrug resistance phenotype	869:921	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	5	62	from	alterations	990:1000	arg1	glycomarkers					1051:1062	glycomarkers	1051:1062	glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes	1051:1133	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	5	62	from	alterations	990:1000	arg1	glycophenotype					1014:1027	the cell glycophenotype	1005:1027	the cell glycophenotype	1005:1027	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	5	63	theme	epithelial-to-mesenchymal	1088:1112	arg1	transition					1114:1123	epithelial-to-mesenchymal transition	1088:1123	epithelial-to-mesenchymal transition	1088:1123	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	6	64	theme	continuous	1249:1258	arg1	treatment					1260:1268	the continuous treatment	1245:1268	the continuous treatment with the drug paclitaxel	1245:1293	Herein, we induced the multidrug resistance phenotype in the PC-3 human prostate adenocarcinoma line through the continuous treatment with the drug paclitaxel.					
32914709	5	65	theme	multidrug	892:900	arg1	phenotype					913:921	the multidrug resistance phenotype	888:921	the multidrug resistance phenotype	888:921	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	7	66	theme	cell	1332:1335	arg1	phenotype					1358:1366	the induced cell multidrug resistance phenotype	1320:1366	the induced cell multidrug resistance phenotype	1320:1366	Our results showed that the induced cell multidrug resistance phenotype (1) acquired a mixed profile between epithelial and mesenchymal phenotypes and (2) modified the glycophenotype, showing an increase in the level of sialylation and in the number of branched glycans.					
32914709	5	67	theme	cell	1009:1012	arg1	glycomarkers					1051:1062	glycomarkers	1051:1062	glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes	1051:1133	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	5	67	theme	cell	1009:1012	arg1	glycophenotype					1014:1027	the cell glycophenotype	1005:1027	the cell glycophenotype	1005:1027	We believe that the acquisition of the multidrug resistance phenotype and the activation of epithelial-to-mesenchymal transition provoke alterations in the cell glycophenotype, which can be used as glycomarkers for chemoresistance and epithelial-to-mesenchymal transition processes.					
32914709	3	68	theme	resistance	468:477	arg1	acquisition					453:463	the acquisition	449:463	the acquisition of resistance in cancer	449:487	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	3	69	theme	transition	551:560	arg1	process					562:568	the epithelial-to-mesenchymal transition process	521:568	the epithelial-to-mesenchymal transition process	521:568	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	0	70	theme	transition	86:95	arg1	activation					97:106	mesenchymal-to-epithelial transition activation	60:106	mesenchymal-to-epithelial transition activation	60:106	Resistance to paclitaxel induces glycophenotype changes and mesenchymal-to-epithelial transition activation in the human prostate cancer cell line PC-3.					
32914709	3	71	theme	organs	718:723	arg1	invasion					700:707	the invasion	696:707	the invasion of other organs and tissues	696:735	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	4	72	theme	tumor	814:818	arg1	cells					820:824	tumor cells	814:824	tumor cells	814:824	In addition, it is known that atypical glycosylations are characteristic of tumor cells, being used as biomarkers.					
32914709	3	73	from	acquisition	453:463	arg1	cancer					482:487	cancer	482:487	cancer	482:487	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
32914709	3	74	theme	process	562:568	arg1	activation					507:516	activation	507:516	activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues	507:735	In this work, we hypothesized that the acquisition of resistance in cancer is accompanied by activation of the epithelial-to-mesenchymal transition process, where the tumor cell acquires a more mobile and invasive phenotype; a fundamental step in tumor progression and in promoting the invasion of other organs and tissues.					
33644825	10	0	theme	mannosylated	1563:1574	arg1	oligomannose					1596:1607	oligomannose	1596:1607	oligomannose	1596:1607	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	0	theme	mannosylated	1563:1574	arg1	glycoforms					1576:1585	high mannosylated glycoforms	1558:1585	high mannosylated glycoforms	1558:1585	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	0	theme	mannosylated	1563:1574	arg1	N-glycans					1625:1633	hybrid type N-glycans	1613:1633	hybrid type N-glycans	1613:1633	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	7	1	theme	slight	1086:1091	arg1	accumulation					1106:1117	a slight oligomannose accumulation	1084:1117	a slight oligomannose accumulation in total serum glyco-profiles	1084:1147	Moreover, we have observed a slight oligomannose accumulation in total serum glyco-profiles.					
33644825	2	2	theme	congenital	291:300	arg1	disorder					302:309	a congenital disorder	289:309	Variants in ALG2cause a congenital disorder of glycosylation (CDG)	267:332	Variants in ALG2cause a congenital disorder of glycosylation (CDG) known as ALG2-CDG.					
33644825	5	3	theme	exome	842:846	arg1	sequencing					848:857	exome sequencing	842:857	exome sequencing	842:857	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	8	4	theme	marginal	1232:1239	arg1	disarrangement					1241:1254	a marginal disarrangement	1230:1254	a marginal disarrangement of the glycan processing in ALG2-CDG	1230:1291	The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG.					
33644825	5	5	theme	MALDI-TOF	631:639	arg1	MS					641:642	MALDI-TOF MS	631:642	MALDI-TOF MS	631:642	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	6	6	gly	glycosylation	932:944	arg2	s					951:951	glycosylation site(s)	932:952	glycosylation site(s) typical of a defective N-glycan assembly	932:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	6	6	gly	glycosylation	932:944	arg2	site					946:949	glycosylation site(s)	932:952	glycosylation site(s) typical of a defective N-glycan assembly	932:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	9	7	theme	biosynthesis	1471:1482	arg1	steps					1430:1434	the mannosylation steps	1412:1434	the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1412:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	8	8	theme	serum	1181:1185	arg1	N-glycome					1187:1195	serum N-glycome	1181:1195	serum N-glycome	1181:1195	The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG.					
33644825	10	9	theme	type	1620:1623	arg1	N-glycans					1625:1633	hybrid type N-glycans	1613:1633	hybrid type N-glycans	1613:1633	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	6	10	theme	glycosylation	932:944	arg1	s					951:951	glycosylation site(s)	932:952	glycosylation site(s) typical of a defective N-glycan assembly	932:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	4	11	theme	rare	432:435	arg1	disorder					467:474	a rare autosomal recessive inherited disorder	430:474	a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression	430:605	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	4	11	theme	rare	432:435	arg1	ALG2-CDG					418:425	ALG2-CDG	418:425	ALG2-CDG	418:425	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	8	12	theme	glycan	1263:1268	arg1	processing					1270:1279	the glycan processing	1259:1279	the glycan processing	1259:1279	The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG.					
33644825	1	13	from	synthesis	214:222	arg1	reticulum					256:264	the endoplasmic reticulum	240:264	the endoplasmic reticulum	240:264	Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum.					
33644825	8	14	theme	studied	1204:1210	arg1	patients					1212:1219	the studied patients	1200:1219	the studied patients	1200:1219	The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG.					
33644825	4	15	theme	recessive	447:455	arg1	disorder					467:474	a rare autosomal recessive inherited disorder	430:474	a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression	430:605	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	4	15	theme	recessive	447:455	arg1	ALG2-CDG					418:425	ALG2-CDG	418:425	ALG2-CDG	418:425	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	10	16	theme	potential	1639:1647	arg1	accumulation					1542:1553	serum N-glycan accumulation	1527:1553	serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans,	1527:1634	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	16	theme	potential	1639:1647	arg1	signals					1660:1666	potential diagnostic signals	1639:1666	potential diagnostic signals for ALG2-CDG patients	1639:1688	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	5	17	theme	serum	665:669	arg1	protein					671:677	total serum protein	659:677	total serum protein	659:677	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	5	17	theme	serum	665:669	arg1	Tf					696:697	Tf	696:697	Tf	696:697	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	1	18	theme	Human	129:133	arg1	ALG2					135:138	Human ALG2	129:138	Human ALG2	129:138	Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum.					
33644825	1	18	theme	Human	129:133	arg1	1,3mannosyltransferase					153:174	an α 1,3mannosyltransferase	148:174	an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum	148:264	Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum.					
33644825	6	19	theme	observed	901:908	arg1	under-occupancy					913:927	observed Tf under-occupancy	901:927	observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly	901:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	6	20	theme	assembly	986:993	arg1	typical					954:960	typical	954:960	typical	954:960	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	5	21	theme	homozygous	807:816	arg1	state					818:822	homozygous state	807:822	homozygous state	807:822	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	1	22	theme	α	151:151	arg1	ALG2					135:138	Human ALG2	129:138	Human ALG2	129:138	Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum.					
33644825	1	22	theme	α	151:151	arg1	1,3mannosyltransferase					153:174	an α 1,3mannosyltransferase	148:174	an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum	148:264	Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum.					
33644825	6	23	theme	defective	967:975	arg1	assembly					986:993	a defective N-glycan assembly	965:993	a defective N-glycan assembly	965:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	5	24	contain	carrying	747:754	arg1	patients					738:745	three ALG2-CDG patients	723:745	three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state	723:822	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	5	24	contain	carrying	747:754	arg2	variant					796:802	p.Arg251Leu ALG2 missense variant	770:802	p.Arg251Leu ALG2 missense variant	770:802	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	5	24	contain	carrying	747:754	arg2	c.752G > T					758:767	a c.752G > T	756:767	a c.752G > T	756:767	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	4	25	theme	convulsive	519:528	arg1	syndrome					530:537	convulsive syndrome	519:537	convulsive syndrome of unknown origin	519:555	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	0	26	theme	genetic	97:103	arg1	variant					105:111	a new genetic variant	91:111	a new genetic variant in homozygosis	91:126	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.					
33644825	9	27	theme	Previous	1294:1301	arg1	studies					1303:1309	Previous studies	1294:1309	Previous studies	1294:1309	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	0	28	theme	Argentinean	65:75	arg1	patients					77:84	Argentinean patients	65:84	Argentinean patients with a new genetic variant in homozygosis	65:126	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.					
33644825	6	29	theme	type	1041:1044	arg1	N-glycans					1046:1054	hybrid type N-glycans	1034:1054	hybrid type N-glycans	1034:1054	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	3	30	theme	ALG2-CDG	370:377	arg1	patients					379:386	nine ALG2-CDG patients	365:386	nine ALG2-CDG patients	365:386	Up to date, nine ALG2-CDG patients have been reported worldwide.					
33644825	0	31	theme	Mass	0:3	arg1	characterization					33:48	Mass spectrometry glycophenotype characterization	0:48	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.	0:127	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.					
33644825	9	32	link	dolichol-linked	1439:1453	arg1	biosynthesis					1471:1482	dolichol-linked oligosaccharide biosynthesis	1439:1482	dolichol-linked oligosaccharide biosynthesis	1439:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	4	33	theme	axial	558:562	arg1	hypotonia					564:572	axial hypotonia	558:572	axial hypotonia	558:572	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	10	34	theme	serum	1527:1531	arg1	accumulation					1542:1553	serum N-glycan accumulation	1527:1553	serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans,	1527:1634	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	34	theme	serum	1527:1531	arg1	signals					1660:1666	potential diagnostic signals	1639:1666	potential diagnostic signals for ALG2-CDG patients	1639:1688	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	35	dep	potential	1639:1647	arg1	diagnostic					1649:1658	diagnostic	1649:1658	diagnostic	1649:1658	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	0	36	theme	glycophenotype	18:31	arg1	characterization					33:48	Mass spectrometry glycophenotype characterization	0:48	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.	0:127	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.					
33644825	7	37	theme	total	1122:1126	arg1	glyco-profiles					1134:1147	total serum glyco-profiles	1122:1147	total serum glyco-profiles	1122:1147	Moreover, we have observed a slight oligomannose accumulation in total serum glyco-profiles.					
33644825	9	38	theme	abnormal	1360:1367	arg1	N-glycans					1375:1383	abnormal serum N-glycans	1360:1383	abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1360:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	10	39	theme	high	1558:1561	arg1	oligomannose					1596:1607	oligomannose	1596:1607	oligomannose	1596:1607	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	39	theme	high	1558:1561	arg1	glycoforms					1576:1585	high mannosylated glycoforms	1558:1585	high mannosylated glycoforms	1558:1585	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	39	theme	high	1558:1561	arg1	N-glycans					1625:1633	hybrid type N-glycans	1613:1633	hybrid type N-glycans	1613:1633	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	5	40	theme	ALG2	782:785	arg1	variant					796:802	p.Arg251Leu ALG2 missense variant	770:802	p.Arg251Leu ALG2 missense variant	770:802	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	5	40	theme	ALG2	782:785	arg1	c.752G > T					758:767	a c.752G > T	756:767	a c.752G > T	756:767	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	9	41	theme	N-glycans	1375:1383	arg1	concentrations					1342:1355	slightly increased concentrations	1323:1355	slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1323:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	9	42	from	defects	1401:1407	arg1	steps					1430:1434	the mannosylation steps	1412:1434	the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1412:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	4	43	theme	origin	550:555	arg1	hypotonia					564:572	axial hypotonia	558:572	axial hypotonia	558:572	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	4	43	theme	origin	550:555	arg1	regression					596:605	motor regression	590:605	motor regression	590:605	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	4	43	theme	origin	550:555	arg1	involvement					506:516	neurological involvement	493:516	neurological involvement	493:516	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	4	43	theme	origin	550:555	arg1	syndrome					530:537	convulsive syndrome	519:537	convulsive syndrome of unknown origin	519:555	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	1	44	theme	N-glycans	227:235	arg1	synthesis					214:222	the synthesis	210:222	the synthesis of N-glycans in the endoplasmic reticulum	210:264	Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum.					
33644825	9	45	theme	dolichol-linked	1439:1453	arg1	biosynthesis					1471:1482	dolichol-linked oligosaccharide biosynthesis	1439:1482	dolichol-linked oligosaccharide biosynthesis	1439:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	7	46	theme	oligomannose	1093:1104	arg1	accumulation					1106:1117	a slight oligomannose accumulation	1084:1117	a slight oligomannose accumulation in total serum glyco-profiles	1084:1147	Moreover, we have observed a slight oligomannose accumulation in total serum glyco-profiles.					
33644825	10	47	theme	glycoforms	1576:1585	arg1	accumulation					1542:1553	serum N-glycan accumulation	1527:1553	serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans,	1527:1634	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	47	theme	glycoforms	1576:1585	arg1	signals					1660:1666	potential diagnostic signals	1639:1666	potential diagnostic signals for ALG2-CDG patients	1639:1688	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	7	48	from	accumulation	1106:1117	arg1	glyco-profiles					1134:1147	total serum glyco-profiles	1122:1147	total serum glyco-profiles	1122:1147	Moreover, we have observed a slight oligomannose accumulation in total serum glyco-profiles.					
33644825	8	49	from	heterogeneity	1164:1176	arg1	patients					1212:1219	the studied patients	1200:1219	the studied patients	1200:1219	The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG.					
33644825	10	50	theme	hybrid	1613:1618	arg1	N-glycans					1625:1633	hybrid type N-glycans	1613:1633	hybrid type N-glycans	1613:1633	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	8	51	gly	heterogeneity	1164:1176	arg1	N-glycome					1187:1195	serum N-glycome	1181:1195	serum N-glycome	1181:1195	The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG.					
33644825	9	52	theme	mannosylation	1416:1428	arg1	steps					1430:1434	the mannosylation steps	1412:1434	the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1412:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	6	53	theme	control	876:882	arg1	samples					884:890	control samples	876:890	control samples	876:890	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	4	54	theme	motor	590:594	arg1	regression					596:605	motor regression	590:605	motor regression	590:605	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	0	55	with	patients	77:84	arg1	variant					105:111	a new genetic variant	91:111	a new genetic variant in homozygosis	91:126	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.					
33644825	1	56	theme	first	195:199	arg1	steps					201:205	the first steps	191:205	the first steps in the synthesis of N-glycans in the endoplasmic reticulum	191:264	Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum.					
33644825	9	57	theme	oligosaccharide	1455:1469	arg1	biosynthesis					1471:1482	dolichol-linked oligosaccharide biosynthesis	1439:1482	dolichol-linked oligosaccharide biosynthesis	1439:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	8	58	theme	increased	1154:1162	arg1	heterogeneity					1164:1176	The increased heterogeneity	1150:1176	The increased heterogeneity of serum N-glycome in the studied patients	1150:1219	The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG.					
33644825	6	59	theme	s	951:951	arg1	under-occupancy					913:927	observed Tf under-occupancy	901:927	observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly	901:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	6	59	theme	s	951:951	arg1	occurrence					1003:1012	the occurrence	999:1012	the occurrence of oligomannose and hybrid type N-glycans	999:1054	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	6	60	contain	have	896:899	arg1	we					893:894	we	893:894	we	893:894	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	6	60	contain	have	896:899	arg2	occurrence					1003:1012	the occurrence	999:1012	the occurrence of oligomannose and hybrid type N-glycans	999:1054	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	6	60	contain	have	896:899	arg2	under-occupancy					913:927	observed Tf under-occupancy	901:927	observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly	901:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	5	61	theme	transferrin	683:693	arg1	phenotypes					709:718	both total serum protein and transferrin (Tf) N-glycan phenotypes	654:718	both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state	654:822	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	2	62	theme	glycosylation	314:326	arg1	disorder					302:309	a congenital disorder	289:309	Variants in ALG2cause a congenital disorder of glycosylation (CDG)	267:332	Variants in ALG2cause a congenital disorder of glycosylation (CDG) known as ALG2-CDG.					
33644825	6	63	theme	site	946:949	arg1	s					951:951	glycosylation site(s)	932:952	glycosylation site(s) typical of a defective N-glycan assembly	932:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	4	64	theme	autosomal	437:445	arg1	disorder					467:474	a rare autosomal recessive inherited disorder	430:474	a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression	430:605	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	4	64	theme	autosomal	437:445	arg1	ALG2-CDG					418:425	ALG2-CDG	418:425	ALG2-CDG	418:425	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	2	65	dep	Variants	267:274	arg1	disorder					302:309	a congenital disorder	289:309	Variants in ALG2cause a congenital disorder of glycosylation (CDG)	267:332	Variants in ALG2cause a congenital disorder of glycosylation (CDG) known as ALG2-CDG.					
33644825	8	66	theme	N-glycome	1187:1195	arg1	heterogeneity					1164:1176	The increased heterogeneity	1150:1176	The increased heterogeneity of serum N-glycome in the studied patients	1150:1219	The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG.					
33644825	4	67	theme	inherited	457:465	arg1	disorder					467:474	a rare autosomal recessive inherited disorder	430:474	a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression	430:605	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	4	67	theme	inherited	457:465	arg1	ALG2-CDG					418:425	ALG2-CDG	418:425	ALG2-CDG	418:425	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	5	68	theme	total	659:663	arg1	protein					671:677	total serum protein	659:677	total serum protein	659:677	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	5	68	theme	total	659:663	arg1	Tf					696:697	Tf	696:697	Tf	696:697	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	5	69	from	phenotypes	709:718	arg1	patients					738:745	three ALG2-CDG patients	723:745	three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state	723:822	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	6	70	theme	Tf	910:911	arg1	under-occupancy					913:927	observed Tf under-occupancy	901:927	observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly	901:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	8	71	theme	processing	1270:1279	arg1	disarrangement					1241:1254	a marginal disarrangement	1230:1254	a marginal disarrangement of the glycan processing in ALG2-CDG	1230:1291	The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG.					
33644825	5	72	theme	protein	671:677	arg1	phenotypes					709:718	both total serum protein and transferrin (Tf) N-glycan phenotypes	654:718	both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state	654:822	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	0	73	from	variant	105:111	arg1	homozygosis					116:126	homozygosis	116:126	homozygosis	116:126	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.					
33644825	5	74	theme	ALG2-CDG	729:736	arg1	patients					738:745	three ALG2-CDG patients	723:745	three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state	723:822	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	1	75	from	steps	201:205	arg1	synthesis					214:222	the synthesis	210:222	the synthesis of N-glycans in the endoplasmic reticulum	210:264	Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum.					
33644825	6	76	theme	N-glycan	977:984	arg1	assembly					986:993	a defective N-glycan assembly	965:993	a defective N-glycan assembly	965:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	0	77	from	characterization	33:48	arg1	patients					77:84	Argentinean patients	65:84	Argentinean patients with a new genetic variant in homozygosis	65:126	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.					
33644825	10	78	gly	mannosylated	1563:1574	arg1	oligomannose					1596:1607	oligomannose	1596:1607	oligomannose	1596:1607	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	78	gly	mannosylated	1563:1574	arg1	glycoforms					1576:1585	high mannosylated glycoforms	1558:1585	high mannosylated glycoforms	1558:1585	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	78	gly	mannosylated	1563:1574	arg1	N-glycans					1625:1633	hybrid type N-glycans	1613:1633	hybrid type N-glycans	1613:1633	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	0	79	theme	new	93:95	arg1	variant					105:111	a new genetic variant	91:111	a new genetic variant in homozygosis	91:126	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.					
33644825	9	80	from	N-glycans	1375:1383	arg1	CDG-I					1388:1392	CDG-I	1388:1392	CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1388:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	8	81	from	disarrangement	1241:1254	arg1	ALG2-CDG					1284:1291	ALG2-CDG	1284:1291	ALG2-CDG	1284:1291	The increased heterogeneity of serum N-glycome in the studied patients suggests a marginal disarrangement of the glycan processing in ALG2-CDG.					
33644825	5	82	theme	N-glycan	700:707	arg1	phenotypes					709:718	both total serum protein and transferrin (Tf) N-glycan phenotypes	654:718	both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state	654:822	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	6	83	theme	typical	954:960	arg1	s					951:951	glycosylation site(s)	932:952	glycosylation site(s) typical of a defective N-glycan assembly	932:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	9	84	from	concentrations	1342:1355	arg1	CDG-I					1388:1392	CDG-I	1388:1392	CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1388:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	6	85	theme	N-glycans	1046:1054	arg1	under-occupancy					913:927	observed Tf under-occupancy	901:927	observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly	901:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	6	85	theme	N-glycans	1046:1054	arg1	occurrence					1003:1012	the occurrence	999:1012	the occurrence of oligomannose and hybrid type N-glycans	999:1054	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	4	86	theme	neurological	493:504	arg1	involvement					506:516	neurological involvement	493:516	neurological involvement	493:516	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	10	87	theme	preliminary	1490:1500	arg1	work					1502:1505	This preliminary work	1485:1505	This preliminary work	1485:1505	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	5	88	theme	missense	787:794	arg1	variant					796:802	p.Arg251Leu ALG2 missense variant	770:802	p.Arg251Leu ALG2 missense variant	770:802	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	5	88	theme	missense	787:794	arg1	c.752G > T					758:767	a c.752G > T	756:767	a c.752G > T	756:767	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	6	89	theme	hybrid	1034:1039	arg1	N-glycans					1046:1054	hybrid type N-glycans	1034:1054	hybrid type N-glycans	1034:1054	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	10	90	theme	ALG2-CDG	1672:1679	arg1	patients					1681:1688	ALG2-CDG patients	1672:1688	ALG2-CDG patients	1672:1688	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	7	91	theme	serum	1128:1132	arg1	glyco-profiles					1134:1147	total serum glyco-profiles	1122:1147	total serum glyco-profiles	1122:1147	Moreover, we have observed a slight oligomannose accumulation in total serum glyco-profiles.					
33644825	9	92	theme	increased	1332:1340	arg1	concentrations					1342:1355	slightly increased concentrations	1323:1355	slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1323:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	6	93	theme	oligomannose	1017:1028	arg1	under-occupancy					913:927	observed Tf under-occupancy	901:927	observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly	901:993	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	6	93	theme	oligomannose	1017:1028	arg1	occurrence					1003:1012	the occurrence	999:1012	the occurrence of oligomannose and hybrid type N-glycans	999:1054	Comparing it to control samples, we have observed Tf under-occupancy of glycosylation site(s) typical of a defective N-glycan assembly and the occurrence of oligomannose and hybrid type N-glycans.					
33644825	0	94	theme	ALG2-CDG	53:60	arg1	characterization					33:48	Mass spectrometry glycophenotype characterization	0:48	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.	0:127	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.					
33644825	2	95	from	Variants	267:274	arg1	ALG2cause					279:287	ALG2cause	279:287	ALG2cause	279:287	Variants in ALG2cause a congenital disorder of glycosylation (CDG) known as ALG2-CDG.					
33644825	4	96	theme	unknown	542:548	arg1	origin					550:555	unknown origin	542:555	unknown origin	542:555	ALG2-CDG is a rare autosomal recessive inherited disorder characterized by neurological involvement, convulsive syndrome of unknown origin, axial hypotonia, and mental and motor regression.					
33644825	5	97	theme	p.Arg251Leu	770:780	arg1	variant					796:802	p.Arg251Leu ALG2 missense variant	770:802	p.Arg251Leu ALG2 missense variant	770:802	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	5	97	theme	p.Arg251Leu	770:780	arg1	c.752G > T					758:767	a c.752G > T	756:767	a c.752G > T	756:767	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	9	98	theme	serum	1369:1373	arg1	N-glycans					1375:1383	abnormal serum N-glycans	1360:1383	abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1360:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	5	99	used	used	626:629	arg2	we					623:624	we	623:624	we	623:624	In this study, we used MALDI-TOF MS to define both total serum protein and transferrin (Tf) N-glycan phenotypes in three ALG2-CDG patients carrying a c.752G > T, p.Arg251Leu ALG2 missense variant in homozygous state, as determined by exome sequencing.					
33644825	9	100	from	CDG-I	1388:1392	arg1	concentrations					1342:1355	slightly increased concentrations	1323:1355	slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1323:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	10	101	theme	N-glycan	1533:1540	arg1	accumulation					1542:1553	serum N-glycan accumulation	1527:1553	serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans,	1527:1634	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	10	101	theme	N-glycan	1533:1540	arg1	signals					1660:1666	potential diagnostic signals	1639:1666	potential diagnostic signals for ALG2-CDG patients	1639:1688	This preliminary work aims at considering serum N-glycan accumulation of high mannosylated glycoforms, such as oligomannose and hybrid type N-glycans, as potential diagnostic signals for ALG2-CDG patients.					
33644825	9	102	theme	due	1394:1396	arg1	CDG-I					1388:1392	CDG-I	1388:1392	CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis	1388:1482	Previous studies reported on slightly increased concentrations of abnormal serum N-glycans in CDG-I due to defects in the mannosylation steps of dolichol-linked oligosaccharide biosynthesis.					
33644825	0	103	theme	spectrometry	5:16	arg1	characterization					33:48	Mass spectrometry glycophenotype characterization	0:48	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.	0:127	Mass spectrometry glycophenotype characterization of ALG2-CDG in Argentinean patients with a new genetic variant in homozygosis.					
33644825	1	104	theme	endoplasmic	244:254	arg1	reticulum					256:264	the endoplasmic reticulum	240:264	the endoplasmic reticulum	240:264	Human ALG2 encodes an α 1,3mannosyltransferase that catalyzes the first steps in the synthesis of N-glycans in the endoplasmic reticulum.					
32560600	4	0	theme	laser	730:734	arg1	MALDI-TOF-MS					792:803	MALDI-TOF-MS	792:803	MALDI-TOF-MS	792:803	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	0	theme	laser	730:734	arg1	spectrometry					778:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	714:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	714:804	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	1	1	theme	flow	151:154	arg1	system					166:171	a flow chemistry system	149:171	a flow chemistry system	149:171	This study demonstrates the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling to assist with the existing methods in glycan structural analysis.					
32560600	1	2	theme	existing	249:256	arg1	methods					258:264	the existing methods	245:264	the existing methods	245:264	This study demonstrates the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling to assist with the existing methods in glycan structural analysis.					
32560600	0	3	theme	Saccharides	69:79	arg1	Labeling					57:64	Rapid Labeling	51:64	Rapid Labeling of Saccharides after Glycan Hydrolysis	51:103	Flow Chemistry System for Carbohydrate Analysis by Rapid Labeling of Saccharides after Glycan Hydrolysis.					
32560600	2	4	theme	flow	352:355	arg1	system					357:362	a flow system	350:362	a flow system	350:362	Acidic hydrolysis of glycans could be accelerated in a flow system.					
32560600	2	5	theme	Acidic	297:302	arg1	hydrolysis					304:313	Acidic hydrolysis	297:313	Acidic hydrolysis of glycans	297:324	Acidic hydrolysis of glycans could be accelerated in a flow system.					
32560600	4	6	theme	NADA-labeled	588:599	arg1	derivatives					601:611	The NADA-labeled derivatives	584:611	The NADA-labeled derivatives	584:611	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	0	7	theme	Glycan	87:92	arg1	Hydrolysis					94:103	Glycan Hydrolysis	87:103	Glycan Hydrolysis	87:103	Flow Chemistry System for Carbohydrate Analysis by Rapid Labeling of Saccharides after Glycan Hydrolysis.					
32560600	5	8	theme	possible	994:1001	arg1	permutations					1003:1014	six possible permutations	990:1014	six possible permutations	990:1014	Furthermore, this protocol was applied to determine the SA-Gal-Glc sequence of GM3-sugar out of six possible permutations.					
32560600	3	9	theme	fluorescent	498:508	arg1	naphthimidazole					510:524	the fluorescent naphthimidazole	494:524	the fluorescent naphthimidazole (NAIM)	494:531	Aldoses and α-ketoacid-type saccharides were effectively labeled with naphthalene-2,3-diamine (NADA) at 60 °C for 10 min to form the fluorescent naphthimidazole (NAIM) and quinoxalinone (QXO) derivatives, respectively.					
32560600	3	9	theme	fluorescent	498:508	arg1	NAIM					527:530	NAIM	527:530	NAIM	527:530	Aldoses and α-ketoacid-type saccharides were effectively labeled with naphthalene-2,3-diamine (NADA) at 60 °C for 10 min to form the fluorescent naphthimidazole (NAIM) and quinoxalinone (QXO) derivatives, respectively.					
32560600	1	10	theme	glycan	269:274	arg1	analysis					287:294	glycan structural analysis	269:294	glycan structural analysis	269:294	This study demonstrates the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling to assist with the existing methods in glycan structural analysis.					
32560600	4	11	theme	matrix-assisted	714:728	arg1	MALDI-TOF-MS					792:803	MALDI-TOF-MS	792:803	MALDI-TOF-MS	792:803	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	11	theme	matrix-assisted	714:728	arg1	spectrometry					778:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	714:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	714:804	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	5	12	dep	permutations	1003:1014	arg1	out					983:985	out	983:985	out	983:985	Furthermore, this protocol was applied to determine the SA-Gal-Glc sequence of GM3-sugar out of six possible permutations.					
32560600	5	13	theme	GM3-sugar	973:981	arg1	sequence					961:968	the SA-Gal-Glc sequence	946:968	the SA-Gal-Glc sequence of GM3-sugar out of six possible permutations	946:1014	Furthermore, this protocol was applied to determine the SA-Gal-Glc sequence of GM3-sugar out of six possible permutations.					
32560600	4	14	theme	mass	829:832	arg1	LC-MS					848:852	LC-MS	848:852	LC-MS	848:852	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	14	theme	mass	829:832	arg1	spectrometry					834:845	liquid chromatography mass spectrometry	807:845	liquid chromatography mass spectrometry (LC-MS)	807:853	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	1	15	theme	system	166:171	arg1	utilization					134:144	the utilization	130:144	the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling	130:228	This study demonstrates the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling to assist with the existing methods in glycan structural analysis.					
32560600	4	16	theme	chromatography	814:827	arg1	LC-MS					848:852	LC-MS	848:852	LC-MS	848:852	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	16	theme	chromatography	814:827	arg1	spectrometry					834:845	liquid chromatography mass spectrometry	807:845	liquid chromatography mass spectrometry (LC-MS)	807:853	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	1	17	theme	continuous	177:186	arg1	hydrolysis					195:204	continuous glycan hydrolysis	177:204	continuous glycan hydrolysis	177:204	This study demonstrates the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling to assist with the existing methods in glycan structural analysis.					
32560600	0	18	theme	Flow	0:3	arg1	Chemistry					5:13	Flow Chemistry	0:13	Flow Chemistry	0:13	Flow Chemistry System for Carbohydrate Analysis by Rapid Labeling of Saccharides after Glycan Hydrolysis.					
32560600	1	19	theme	structural	276:285	arg1	analysis					287:294	glycan structural analysis	269:294	glycan structural analysis	269:294	This study demonstrates the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling to assist with the existing methods in glycan structural analysis.					
32560600	2	20	theme	glycans	318:324	arg1	hydrolysis					304:313	Acidic hydrolysis	297:313	Acidic hydrolysis of glycans	297:324	Acidic hydrolysis of glycans could be accelerated in a flow system.					
32560600	4	21	theme	liquid	807:812	arg1	LC-MS					848:852	LC-MS	848:852	LC-MS	848:852	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	21	theme	liquid	807:812	arg1	spectrometry					834:845	liquid chromatography mass spectrometry	807:845	liquid chromatography mass spectrometry (LC-MS)	807:853	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	1	22	theme	glycan	188:193	arg1	hydrolysis					195:204	continuous glycan hydrolysis	177:204	continuous glycan hydrolysis	177:204	This study demonstrates the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling to assist with the existing methods in glycan structural analysis.					
32560600	4	23	dep	determination	637:649	arg1	the					622:624	the	622:624	the	622:624	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	3	24	theme	QXO	552:554	arg1	derivatives					557:567	quinoxalinone (QXO) derivatives	537:567	quinoxalinone (QXO) derivatives	537:567	Aldoses and α-ketoacid-type saccharides were effectively labeled with naphthalene-2,3-diamine (NADA) at 60 °C for 10 min to form the fluorescent naphthimidazole (NAIM) and quinoxalinone (QXO) derivatives, respectively.					
32560600	3	25	theme	α-ketoacid-type	377:391	arg1	saccharides					393:403	α-ketoacid-type saccharides	377:403	α-ketoacid-type saccharides	377:403	Aldoses and α-ketoacid-type saccharides were effectively labeled with naphthalene-2,3-diamine (NADA) at 60 °C for 10 min to form the fluorescent naphthimidazole (NAIM) and quinoxalinone (QXO) derivatives, respectively.					
32560600	5	26	theme	permutations	1003:1014	arg1	sequence					961:968	the SA-Gal-Glc sequence	946:968	the SA-Gal-Glc sequence of GM3-sugar out of six possible permutations	946:1014	Furthermore, this protocol was applied to determine the SA-Gal-Glc sequence of GM3-sugar out of six possible permutations.					
32560600	1	27	theme	chemistry	156:164	arg1	system					166:171	a flow chemistry system	149:171	a flow chemistry system	149:171	This study demonstrates the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling to assist with the existing methods in glycan structural analysis.					
32560600	4	28	theme	parent	686:691	arg1	saccharides					693:703	their parent saccharides	680:703	their parent saccharides	680:703	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	1	29	theme	saccharide	210:219	arg1	labeling					221:228	saccharide labeling	210:228	saccharide labeling	210:228	This study demonstrates the utilization of a flow chemistry system for continuous glycan hydrolysis and saccharide labeling to assist with the existing methods in glycan structural analysis.					
32560600	0	30	theme	Carbohydrate	26:37	arg1	Analysis					39:46	Carbohydrate Analysis	26:46	Carbohydrate Analysis by Rapid Labeling of Saccharides after Glycan Hydrolysis	26:103	Flow Chemistry System for Carbohydrate Analysis by Rapid Labeling of Saccharides after Glycan Hydrolysis.					
32560600	4	31	theme	composition	655:665	arg1	analysis					667:674	composition analysis	655:674	composition analysis	655:674	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	0	32	theme	Rapid	51:55	arg1	Labeling					57:64	Rapid Labeling	51:64	Rapid Labeling of Saccharides after Glycan Hydrolysis	51:103	Flow Chemistry System for Carbohydrate Analysis by Rapid Labeling of Saccharides after Glycan Hydrolysis.					
32560600	4	33	theme	magnetic	868:875	arg1	NMR					888:890	NMR	888:890	NMR	888:890	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	33	theme	magnetic	868:875	arg1	resonance					877:885	nuclear magnetic resonance	860:885	nuclear magnetic resonance (NMR)	860:891	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	34	theme	ionization	747:756	arg1	MALDI-TOF-MS					792:803	MALDI-TOF-MS	792:803	MALDI-TOF-MS	792:803	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	34	theme	ionization	747:756	arg1	spectrometry					778:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	714:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	714:804	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	35	theme	nuclear	860:866	arg1	NMR					888:890	NMR	888:890	NMR	888:890	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	35	theme	nuclear	860:866	arg1	resonance					877:885	nuclear magnetic resonance	860:885	nuclear magnetic resonance (NMR)	860:891	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	36	theme	desorption	736:745	arg1	MALDI-TOF-MS					792:803	MALDI-TOF-MS	792:803	MALDI-TOF-MS	792:803	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	36	theme	desorption	736:745	arg1	spectrometry					778:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	714:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	714:804	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	37	theme	mass	773:776	arg1	MALDI-TOF-MS					792:803	MALDI-TOF-MS	792:803	MALDI-TOF-MS	792:803	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	37	theme	mass	773:776	arg1	spectrometry					778:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	714:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	714:804	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	3	38	theme	quinoxalinone	537:549	arg1	derivatives					557:567	quinoxalinone (QXO) derivatives	537:567	quinoxalinone (QXO) derivatives	537:567	Aldoses and α-ketoacid-type saccharides were effectively labeled with naphthalene-2,3-diamine (NADA) at 60 °C for 10 min to form the fluorescent naphthimidazole (NAIM) and quinoxalinone (QXO) derivatives, respectively.					
32560600	4	39	theme	time-of-flight	758:771	arg1	MALDI-TOF-MS					792:803	MALDI-TOF-MS	792:803	MALDI-TOF-MS	792:803	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	4	39	theme	time-of-flight	758:771	arg1	spectrometry					778:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry	714:789	matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	714:804	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
32560600	5	40	theme	SA-Gal-Glc	950:959	arg1	sequence					961:968	the SA-Gal-Glc sequence	946:968	the SA-Gal-Glc sequence of GM3-sugar out of six possible permutations	946:1014	Furthermore, this protocol was applied to determine the SA-Gal-Glc sequence of GM3-sugar out of six possible permutations.					
32560600	4	41	theme	structural	626:635	arg1	determination					637:649	structural determination	626:649	structural determination	626:649	The NADA-labeled derivatives improved the structural determination and composition analysis for their parent saccharides by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), liquid chromatography mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR).					
33507566	8	0	theme	peak	1076:1079	arg1	abundance					1063:1071	The abundance	1059:1071	The abundance of peak 5 (NA2)	1059:1087	The abundance of peak 5 (NA2) increased gradually with the aggravation of disease severity though there was no statistically significant, was probably correlated with the disease severity.					
33507566	9	1	theme	%	1452:1452	arg1	specificity					1432:1442	a specificity	1430:1442	a specificity of 85.53%	1430:1452	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	1	theme	%	1452:1452	arg1	value					1521:1525	a negative predictive value	1499:1525	a negative predictive value(NPV) of 75.58%	1499:1540	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	1	theme	%	1452:1452	arg1	sensitivity					1407:1417	a sensitivity	1405:1417	a sensitivity of 72.37%	1405:1427	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	1	theme	%	1452:1452	arg1	value					1477:1481	a positive predictive value	1455:1481	a positive predictive value(PPV) of 83.33%	1455:1496	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	1	theme	%	1452:1452	arg1	NPV					1527:1529	NPV	1527:1529	NPV	1527:1529	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	1	theme	%	1452:1452	arg1	PPV					1483:1485	PPV	1483:1485	PPV	1483:1485	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	1	theme	%	1452:1452	arg1	accuracy					1550:1557	an accuracy	1547:1557	an accuracy of 78.95%	1547:1567	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	5	2	theme	same	598:601	arg1	time					603:606	the same time	594:606	the same time	594:606	At the same time, 76 healthy subjects were enrolled as a control group.					
33507566	7	3	from	peaks	876:880	arg1	abundance					849:857	the relative abundance	836:857	the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb)	836:921	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	3	from	peaks	876:880	arg1	p < .05					937:943	p < .05	937:943	p < .05	937:943	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	3	from	peaks	876:880	arg1	elevated					927:934	elevated	927:934	elevated	927:934	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	9	4	theme	%	1427:1427	arg1	specificity					1432:1442	a specificity	1430:1442	a specificity of 85.53%	1430:1452	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	4	theme	%	1427:1427	arg1	value					1521:1525	a negative predictive value	1499:1525	a negative predictive value(NPV) of 75.58%	1499:1540	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	4	theme	%	1427:1427	arg1	sensitivity					1407:1417	a sensitivity	1405:1417	a sensitivity of 72.37%	1405:1427	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	4	theme	%	1427:1427	arg1	value					1477:1481	a positive predictive value	1455:1481	a positive predictive value(PPV) of 83.33%	1455:1496	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	4	theme	%	1427:1427	arg1	NPV					1527:1529	NPV	1527:1529	NPV	1527:1529	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	4	theme	%	1427:1427	arg1	PPV					1483:1485	PPV	1483:1485	PPV	1483:1485	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	4	theme	%	1427:1427	arg1	accuracy					1550:1557	an accuracy	1547:1557	an accuracy of 78.95%	1547:1567	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	7	5	dep	peaks	876:880	arg1	NA4					903:905	NA4	903:905	NA4	903:905	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	5	dep	peaks	876:880	arg1	NA4Fb					916:920	NA4Fb	916:920	NA4Fb	916:920	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	5	dep	peaks	876:880	arg1	9					890:890	9	890:890	9	890:890	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	5	dep	peaks	876:880	arg1	5					882:882	5	882:882	5	882:882	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	5	dep	peaks	876:880	arg1	NA3Fb					892:896	NA3Fb	892:896	NA3Fb	892:896	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	5	dep	peaks	876:880	arg1	NA2					884:886	NA2	884:886	NA2	884:886	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	5	dep	peaks	876:880	arg1	11					900:901	11	900:901	11	900:901	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	2	6	from	change	192:197	arg1	glycosylation					202:214	glycosylation	202:214	glycosylation	202:214	The change in glycosylation is involved in the occurrence and development of various diseases, and this study verified that N-glycan markers might be a diagnostic marker in psoriasis.					
33507566	8	7	theme	disease	1230:1236	arg1	severity					1238:1245	the disease severity	1226:1245	the disease severity	1226:1245	The abundance of peak 5 (NA2) increased gradually with the aggravation of disease severity though there was no statistically significant, was probably correlated with the disease severity.					
33507566	1	8	theme	protein	179:185	arg1	modification					163:174	an important post-translational modification	131:174	an important post-translational modification of protein	131:185	BACKGROUND Glycosylation is an important post-translational modification of protein.					
33507566	1	8	theme	protein	179:185	arg1	Glycosylation					114:126	BACKGROUND Glycosylation	103:126	BACKGROUND Glycosylation	103:126	BACKGROUND Glycosylation is an important post-translational modification of protein.					
33507566	0	9	theme	psoriasis	92:100	arg1	assessment					78:87	assessment	78:87	assessment	78:87	Serum N-glycan profiling as a diagnostic biomarker for the identification and assessment of psoriasis.					
33507566	0	9	theme	psoriasis	92:100	arg1	identification					59:72	identification	59:72	identification	59:72	Serum N-glycan profiling as a diagnostic biomarker for the identification and assessment of psoriasis.					
33507566	2	10	theme	diseases	273:280	arg1	development					250:260	development	250:260	development	250:260	The change in glycosylation is involved in the occurrence and development of various diseases, and this study verified that N-glycan markers might be a diagnostic marker in psoriasis.					
33507566	2	10	theme	diseases	273:280	arg1	occurrence					235:244	occurrence	235:244	occurrence	235:244	The change in glycosylation is involved in the occurrence and development of various diseases, and this study verified that N-glycan markers might be a diagnostic marker in psoriasis.					
33507566	6	11	theme	serum	766:770	arg1	profiling					781:789	serum N-glycan profiling	766:789	serum N-glycan profiling	766:789	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	9	12	theme	operating	1281:1289	arg1	curve					1312:1316	the receiver operating characteristic (ROC) curve	1268:1316	the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA)	1268:1365	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	12	theme	operating	1281:1289	arg1	AUC					1319:1321	AUC	1319:1321	AUC	1319:1321	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	7	13	theme	relative	840:847	arg1	abundance					849:857	the relative abundance	836:857	the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb)	836:921	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	13	theme	relative	840:847	arg1	p < .05					937:943	p < .05	937:943	p < .05	937:943	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	13	theme	relative	840:847	arg1	elevated					927:934	elevated	927:934	elevated	927:934	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	4	14	theme	mild	563:566	arg1	subgroups					552:560	three subgroups	546:560	three subgroups: mild, moderate, and severe	546:588	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	9	15	theme	positive	1457:1464	arg1	PPV					1483:1485	PPV	1483:1485	PPV	1483:1485	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	15	theme	positive	1457:1464	arg1	value					1477:1481	a positive predictive value	1455:1481	a positive predictive value(PPV) of 83.33%	1455:1496	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	10	16	dep	CONCLUSIONS	1570:1580	arg1	indicated					1592:1600	indicated	1592:1600	indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis	1592:1720	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	2	17	from	marker	351:356	arg1	psoriasis					361:369	psoriasis	361:369	psoriasis	361:369	The change in glycosylation is involved in the occurrence and development of various diseases, and this study verified that N-glycan markers might be a diagnostic marker in psoriasis.					
33507566	9	18	theme	characteristic	1291:1304	arg1	curve					1312:1316	the receiver operating characteristic (ROC) curve	1268:1316	the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA)	1268:1365	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	18	theme	characteristic	1291:1304	arg1	AUC					1319:1321	AUC	1319:1321	AUC	1319:1321	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	19	theme	%	1540:1540	arg1	specificity					1432:1442	a specificity	1430:1442	a specificity of 85.53%	1430:1452	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	19	theme	%	1540:1540	arg1	value					1521:1525	a negative predictive value	1499:1525	a negative predictive value(NPV) of 75.58%	1499:1540	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	19	theme	%	1540:1540	arg1	sensitivity					1407:1417	a sensitivity	1405:1417	a sensitivity of 72.37%	1405:1427	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	19	theme	%	1540:1540	arg1	value					1477:1481	a positive predictive value	1455:1481	a positive predictive value(PPV) of 83.33%	1455:1496	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	19	theme	%	1540:1540	arg1	NPV					1527:1529	NPV	1527:1529	NPV	1527:1529	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	19	theme	%	1540:1540	arg1	PPV					1483:1485	PPV	1483:1485	PPV	1483:1485	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	19	theme	%	1540:1540	arg1	accuracy					1550:1557	an accuracy	1547:1557	an accuracy of 78.95%	1547:1567	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	20	theme	best	1252:1255	arg1	area					1257:1260	The best area	1248:1260	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA)	1248:1365	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	21	theme	predictive	1466:1475	arg1	PPV					1483:1485	PPV	1483:1485	PPV	1483:1485	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	21	theme	predictive	1466:1475	arg1	value					1477:1481	a positive predictive value	1455:1481	a positive predictive value(PPV) of 83.33%	1455:1496	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	6	22	theme	sequencer-assisted	675:692	arg1	DSA-FACE					745:752	DSA-FACE	745:752	DSA-FACE	745:752	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	6	22	theme	sequencer-assisted	675:692	arg1	electrophoresis					728:742	DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis	671:742	DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE)	671:753	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	7	23	from	abundance	849:857	arg1	peaks					876:880	peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb)	876:921	peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb)	876:921	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	10	24	theme	valuable	1659:1666	arg1	alternative					1685:1695	a new, valuable, and noninvasive alternative	1652:1695	a new, valuable, and noninvasive alternative for diagnosing psoriasis	1652:1720	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	10	24	theme	valuable	1659:1666	arg1	model					1637:1641	the N-glycan-based diagnostic model	1607:1641	the N-glycan-based diagnostic model	1607:1641	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	11	25	theme	N-glycan	1767:1774	arg1	marker					1776:1781	the characteristic distinctive N-glycan marker	1736:1781	the characteristic distinctive N-glycan marker	1736:1781	Furthermore, the characteristic distinctive N-glycan marker might be correlated with the severity gradation of the psoriasis disease.					
33507566	9	26	theme	ROC	1307:1309	arg1	curve					1312:1316	the receiver operating characteristic (ROC) curve	1268:1316	the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA)	1268:1365	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	26	theme	ROC	1307:1309	arg1	AUC					1319:1321	AUC	1319:1321	AUC	1319:1321	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	11	27	theme	disease	1848:1854	arg1	gradation					1821:1829	the severity gradation	1808:1829	the severity gradation of the psoriasis disease	1808:1854	Furthermore, the characteristic distinctive N-glycan marker might be correlated with the severity gradation of the psoriasis disease.					
33507566	0	28	theme	N-glycan	6:13	arg1	profiling					15:23	Serum N-glycan profiling	0:23	Serum N-glycan profiling	0:23	Serum N-glycan profiling as a diagnostic biomarker for the identification and assessment of psoriasis.					
33507566	7	29	dep	decreased	1015:1023	arg1	p < .05					1026:1032	p < .05	1026:1032	p < .05	1026:1032	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	11	30	theme	distinctive	1755:1765	arg1	marker					1776:1781	the characteristic distinctive N-glycan marker	1736:1781	the characteristic distinctive N-glycan marker	1736:1781	Furthermore, the characteristic distinctive N-glycan marker might be correlated with the severity gradation of the psoriasis disease.					
33507566	7	31	from	structures	862:871	arg1	peaks					876:880	peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb)	876:921	peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb)	876:921	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	32	theme	healthy	818:824	arg1	controls					826:833	the healthy controls	814:833	the healthy controls	814:833	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	0	33	theme	Serum	0:4	arg1	profiling					15:23	Serum N-glycan profiling	0:23	Serum N-glycan profiling	0:23	Serum N-glycan profiling as a diagnostic biomarker for the identification and assessment of psoriasis.					
33507566	9	34	theme	model	1351:1355	arg1	curve					1312:1316	the receiver operating characteristic (ROC) curve	1268:1316	the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA)	1268:1365	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	34	theme	model	1351:1355	arg1	AUC					1319:1321	AUC	1319:1321	AUC	1319:1321	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	11	35	theme	severity	1812:1819	arg1	gradation					1821:1829	the severity gradation	1808:1829	the severity gradation of the psoriasis disease	1808:1854	Furthermore, the characteristic distinctive N-glycan marker might be correlated with the severity gradation of the psoriasis disease.					
33507566	11	36	theme	characteristic	1740:1753	arg1	marker					1776:1781	the characteristic distinctive N-glycan marker	1736:1781	the characteristic distinctive N-glycan marker	1736:1781	Furthermore, the characteristic distinctive N-glycan marker might be correlated with the severity gradation of the psoriasis disease.					
33507566	9	37	theme	logistic	1331:1338	arg1	model					1351:1355	the logistic regression model	1327:1355	the logistic regression model (PglycoA)	1327:1365	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	37	theme	logistic	1331:1338	arg1	PglycoA					1358:1364	PglycoA	1358:1364	PglycoA	1358:1364	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	2	38	theme	N-glycan	312:319	arg1	marker					351:356	a diagnostic marker	338:356	a diagnostic marker in psoriasis	338:369	The change in glycosylation is involved in the occurrence and development of various diseases, and this study verified that N-glycan markers might be a diagnostic marker in psoriasis.					
33507566	2	38	theme	N-glycan	312:319	arg1	markers					321:327	N-glycan markers	312:327	N-glycan markers	312:327	The change in glycosylation is involved in the occurrence and development of various diseases, and this study verified that N-glycan markers might be a diagnostic marker in psoriasis.					
33507566	2	39	dep	occurrence	235:244	arg1	the					231:233	the	231:233	the	231:233	The change in glycosylation is involved in the occurrence and development of various diseases, and this study verified that N-glycan markers might be a diagnostic marker in psoriasis.					
33507566	4	40	theme	Severity	452:459	arg1	PASI					468:471	PASI	468:471	PASI	468:471	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	4	40	theme	Severity	452:459	arg1	Index					461:465	Psoriasis Area Severity Index	437:465	Psoriasis Area Severity Index (PASI)	437:472	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	1	41	theme	BACKGROUND	103:112	arg1	modification					163:174	an important post-translational modification	131:174	an important post-translational modification of protein	131:185	BACKGROUND Glycosylation is an important post-translational modification of protein.					
33507566	1	41	theme	BACKGROUND	103:112	arg1	Glycosylation					114:126	BACKGROUND Glycosylation	103:126	BACKGROUND Glycosylation	103:126	BACKGROUND Glycosylation is an important post-translational modification of protein.					
33507566	9	42	theme	receiver	1272:1279	arg1	curve					1312:1316	the receiver operating characteristic (ROC) curve	1268:1316	the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA)	1268:1365	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	42	theme	receiver	1272:1279	arg1	AUC					1319:1321	AUC	1319:1321	AUC	1319:1321	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	4	43	theme	severe	583:588	arg1	subgroups					552:560	three subgroups	546:560	three subgroups: mild, moderate, and severe	546:588	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	0	44	theme	diagnostic	30:39	arg1	biomarker					41:49	a diagnostic biomarker	28:49	a diagnostic biomarker for the identification and assessment of psoriasis	28:100	Serum N-glycan profiling as a diagnostic biomarker for the identification and assessment of psoriasis.					
33507566	5	45	theme	control	648:654	arg1	subjects					620:627	76 healthy subjects	609:627	76 healthy subjects	609:627	At the same time, 76 healthy subjects were enrolled as a control group.					
33507566	5	45	theme	control	648:654	arg1	group					656:660	a control group	646:660	a control group	646:660	At the same time, 76 healthy subjects were enrolled as a control group.					
33507566	4	46	theme	Area	447:450	arg1	PASI					468:471	PASI	468:471	PASI	468:471	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	4	46	theme	Area	447:450	arg1	Index					461:465	Psoriasis Area Severity Index	437:465	Psoriasis Area Severity Index (PASI)	437:472	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	8	47	theme	disease	1133:1139	arg1	severity					1141:1148	disease severity	1133:1148	disease severity	1133:1148	The abundance of peak 5 (NA2) increased gradually with the aggravation of disease severity though there was no statistically significant, was probably correlated with the disease severity.					
33507566	9	48	theme	%	1567:1567	arg1	specificity					1432:1442	a specificity	1430:1442	a specificity of 85.53%	1430:1452	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	48	theme	%	1567:1567	arg1	value					1521:1525	a negative predictive value	1499:1525	a negative predictive value(NPV) of 75.58%	1499:1540	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	48	theme	%	1567:1567	arg1	sensitivity					1407:1417	a sensitivity	1405:1417	a sensitivity of 72.37%	1405:1427	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	48	theme	%	1567:1567	arg1	value					1477:1481	a positive predictive value	1455:1481	a positive predictive value(PPV) of 83.33%	1455:1496	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	48	theme	%	1567:1567	arg1	NPV					1527:1529	NPV	1527:1529	NPV	1527:1529	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	48	theme	%	1567:1567	arg1	PPV					1483:1485	PPV	1483:1485	PPV	1483:1485	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	48	theme	%	1567:1567	arg1	accuracy					1550:1557	an accuracy	1547:1557	an accuracy of 78.95%	1547:1567	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	7	49	theme	psoriasis	1042:1050	arg1	group					1052:1056	the psoriasis group	1038:1056	the psoriasis group	1038:1056	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	9	50	theme	%	1496:1496	arg1	specificity					1432:1442	a specificity	1430:1442	a specificity of 85.53%	1430:1452	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	50	theme	%	1496:1496	arg1	value					1521:1525	a negative predictive value	1499:1525	a negative predictive value(NPV) of 75.58%	1499:1540	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	50	theme	%	1496:1496	arg1	sensitivity					1407:1417	a sensitivity	1405:1417	a sensitivity of 72.37%	1405:1427	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	50	theme	%	1496:1496	arg1	value					1477:1481	a positive predictive value	1455:1481	a positive predictive value(PPV) of 83.33%	1455:1496	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	50	theme	%	1496:1496	arg1	NPV					1527:1529	NPV	1527:1529	NPV	1527:1529	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	50	theme	%	1496:1496	arg1	PPV					1483:1485	PPV	1483:1485	PPV	1483:1485	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	50	theme	%	1496:1496	arg1	accuracy					1550:1557	an accuracy	1547:1557	an accuracy of 78.95%	1547:1567	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	10	51	theme	N-glycan-based	1611:1624	arg1	alternative					1685:1695	a new, valuable, and noninvasive alternative	1652:1695	a new, valuable, and noninvasive alternative for diagnosing psoriasis	1652:1720	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	10	51	theme	N-glycan-based	1611:1624	arg1	model					1637:1641	the N-glycan-based diagnostic model	1607:1641	the N-glycan-based diagnostic model	1607:1641	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	10	52	theme	noninvasive	1673:1683	arg1	alternative					1685:1695	a new, valuable, and noninvasive alternative	1652:1695	a new, valuable, and noninvasive alternative for diagnosing psoriasis	1652:1720	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	10	52	theme	noninvasive	1673:1683	arg1	model					1637:1641	the N-glycan-based diagnostic model	1607:1641	the N-glycan-based diagnostic model	1607:1641	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	9	53	with	0.867	1393:1397	arg1	specificity					1432:1442	a specificity	1430:1442	a specificity of 85.53%	1430:1452	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	53	with	0.867	1393:1397	arg1	value					1521:1525	a negative predictive value	1499:1525	a negative predictive value(NPV) of 75.58%	1499:1540	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	53	with	0.867	1393:1397	arg1	sensitivity					1407:1417	a sensitivity	1405:1417	a sensitivity of 72.37%	1405:1427	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	53	with	0.867	1393:1397	arg1	value					1477:1481	a positive predictive value	1455:1481	a positive predictive value(PPV) of 83.33%	1455:1496	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	53	with	0.867	1393:1397	arg1	NPV					1527:1529	NPV	1527:1529	NPV	1527:1529	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	53	with	0.867	1393:1397	arg1	PPV					1483:1485	PPV	1483:1485	PPV	1483:1485	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	53	with	0.867	1393:1397	arg1	accuracy					1550:1557	an accuracy	1547:1557	an accuracy of 78.95%	1547:1567	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	3	54	theme	psoriasis	394:402	arg1	patients					404:411	76 psoriasis patients	391:411	76 psoriasis patients	391:411	METHODS A total of 76 psoriasis patients were recruited.					
33507566	4	55	used	used	432:435	arg2	We					429:430	We	429:430	We	429:430	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	5	56	theme	healthy	612:618	arg1	subjects					620:627	76 healthy subjects	609:627	76 healthy subjects	609:627	At the same time, 76 healthy subjects were enrolled as a control group.					
33507566	5	56	theme	healthy	612:618	arg1	group					656:660	a control group	646:660	a control group	646:660	At the same time, 76 healthy subjects were enrolled as a control group.					
33507566	6	57	theme	carbohydrate	715:726	arg1	DSA-FACE					745:752	DSA-FACE	745:752	DSA-FACE	745:752	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	6	57	theme	carbohydrate	715:726	arg1	electrophoresis					728:742	DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis	671:742	DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE)	671:753	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	3	58	theme	patients	404:411	arg1	total					382:386	A total	380:386	METHODS A total of 76 psoriasis patients	372:411	METHODS A total of 76 psoriasis patients were recruited.					
33507566	8	59	theme	severity	1141:1148	arg1	aggravation					1118:1128	the aggravation	1114:1128	the aggravation of disease severity	1114:1148	The abundance of peak 5 (NA2) increased gradually with the aggravation of disease severity though there was no statistically significant, was probably correlated with the disease severity.					
33507566	6	60	theme	fluorophore-assisted	694:713	arg1	DSA-FACE					745:752	DSA-FACE	745:752	DSA-FACE	745:752	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	6	60	theme	fluorophore-assisted	694:713	arg1	electrophoresis					728:742	DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis	671:742	DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE)	671:753	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	2	61	theme	diagnostic	340:349	arg1	marker					351:356	a diagnostic marker	338:356	a diagnostic marker in psoriasis	338:369	The change in glycosylation is involved in the occurrence and development of various diseases, and this study verified that N-glycan markers might be a diagnostic marker in psoriasis.					
33507566	2	61	theme	diagnostic	340:349	arg1	markers					321:327	N-glycan markers	312:327	N-glycan markers	312:327	The change in glycosylation is involved in the occurrence and development of various diseases, and this study verified that N-glycan markers might be a diagnostic marker in psoriasis.					
33507566	9	62	theme	negative	1501:1508	arg1	value					1521:1525	a negative predictive value	1499:1525	a negative predictive value(NPV) of 75.58%	1499:1540	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	62	theme	negative	1501:1508	arg1	NPV					1527:1529	NPV	1527:1529	NPV	1527:1529	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	8	63	dep	significant	1184:1194	arg1	correlated					1210:1219	correlated	1210:1219	was probably correlated with the disease severity	1197:1245	The abundance of peak 5 (NA2) increased gradually with the aggravation of disease severity though there was no statistically significant, was probably correlated with the disease severity.					
33507566	9	64	theme	regression	1340:1349	arg1	model					1351:1355	the logistic regression model	1327:1355	the logistic regression model (PglycoA)	1327:1365	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	64	theme	regression	1340:1349	arg1	PglycoA					1358:1364	PglycoA	1358:1364	PglycoA	1358:1364	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	6	65	theme	N-glycan	772:779	arg1	profiling					781:789	serum N-glycan profiling	766:789	serum N-glycan profiling	766:789	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	6	66	used	used	666:669	arg2	We					663:664	We	663:664	We	663:664	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	0	67	dep	identification	59:72	arg1	the					55:57	the	55:57	the	55:57	Serum N-glycan profiling as a diagnostic biomarker for the identification and assessment of psoriasis.					
33507566	4	68	theme	moderate	569:576	arg1	subgroups					552:560	three subgroups	546:560	three subgroups: mild, moderate, and severe	546:588	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	3	69	dep	METHODS	372:378	arg1	total					382:386	A total	380:386	METHODS A total of 76 psoriasis patients	372:411	METHODS A total of 76 psoriasis patients were recruited.					
33507566	4	70	theme	Psoriasis	437:445	arg1	PASI					468:471	PASI	468:471	PASI	468:471	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	4	70	theme	Psoriasis	437:445	arg1	Index					461:465	Psoriasis Area Severity Index	437:465	Psoriasis Area Severity Index (PASI)	437:472	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	0	71	dep	profiling	15:23	arg1	biomarker					41:49	a diagnostic biomarker	28:49	a diagnostic biomarker for the identification and assessment of psoriasis	28:100	Serum N-glycan profiling as a diagnostic biomarker for the identification and assessment of psoriasis.					
33507566	6	72	theme	DNA	671:673	arg1	DSA-FACE					745:752	DSA-FACE	745:752	DSA-FACE	745:752	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	6	72	theme	DNA	671:673	arg1	electrophoresis					728:742	DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis	671:742	DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE)	671:753	We used DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis (DSA-FACE) to analyze serum N-glycan profiling.					
33507566	1	73	theme	important	134:142	arg1	modification					163:174	an important post-translational modification	131:174	an important post-translational modification of protein	131:185	BACKGROUND Glycosylation is an important post-translational modification of protein.					
33507566	1	73	theme	important	134:142	arg1	Glycosylation					114:126	BACKGROUND Glycosylation	103:126	BACKGROUND Glycosylation	103:126	BACKGROUND Glycosylation is an important post-translational modification of protein.					
33507566	7	74	theme	structures	862:871	arg1	abundance					849:857	the relative abundance	836:857	the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb)	836:921	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	74	theme	structures	862:871	arg1	p < .05					937:943	p < .05	937:943	p < .05	937:943	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	74	theme	structures	862:871	arg1	elevated					927:934	elevated	927:934	elevated	927:934	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	75	dep	elevated	927:934	arg1	abundance					849:857	the relative abundance	836:857	the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb)	836:921	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	75	dep	elevated	927:934	arg1	p < .05					937:943	p < .05	937:943	p < .05	937:943	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	75	dep	elevated	927:934	arg1	elevated					927:934	elevated	927:934	elevated	927:934	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	2	76	theme	various	265:271	arg1	diseases					273:280	various diseases	265:280	various diseases	265:280	The change in glycosylation is involved in the occurrence and development of various diseases, and this study verified that N-glycan markers might be a diagnostic marker in psoriasis.					
33507566	1	77	theme	post-translational	144:161	arg1	modification					163:174	an important post-translational modification	131:174	an important post-translational modification of protein	131:185	BACKGROUND Glycosylation is an important post-translational modification of protein.					
33507566	1	77	theme	post-translational	144:161	arg1	Glycosylation					114:126	BACKGROUND Glycosylation	103:126	BACKGROUND Glycosylation	103:126	BACKGROUND Glycosylation is an important post-translational modification of protein.					
33507566	9	78	theme	predictive	1510:1519	arg1	value					1521:1525	a negative predictive value	1499:1525	a negative predictive value(NPV) of 75.58%	1499:1540	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	9	78	theme	predictive	1510:1519	arg1	NPV					1527:1529	NPV	1527:1529	NPV	1527:1529	The best area under the receiver operating characteristic (ROC) curve (AUC) of the logistic regression model (PglycoA) to diagnose psoriasis was 0.867, with a sensitivity of 72.37%, a specificity of 85.53%, a positive predictive value(PPV) of 83.33%, a negative predictive value(NPV) of 75.58%, and an accuracy of 78.95%.					
33507566	10	79	theme	new	1654:1656	arg1	alternative					1685:1695	a new, valuable, and noninvasive alternative	1652:1695	a new, valuable, and noninvasive alternative for diagnosing psoriasis	1652:1720	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	10	79	theme	new	1654:1656	arg1	model					1637:1641	the N-glycan-based diagnostic model	1607:1641	the N-glycan-based diagnostic model	1607:1641	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	11	80	theme	psoriasis	1838:1846	arg1	disease					1848:1854	the psoriasis disease	1834:1854	the psoriasis disease	1834:1854	Furthermore, the characteristic distinctive N-glycan marker might be correlated with the severity gradation of the psoriasis disease.					
33507566	10	81	theme	diagnostic	1626:1635	arg1	alternative					1685:1695	a new, valuable, and noninvasive alternative	1652:1695	a new, valuable, and noninvasive alternative for diagnosing psoriasis	1652:1720	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	10	81	theme	diagnostic	1626:1635	arg1	model					1637:1641	the N-glycan-based diagnostic model	1607:1641	the N-glycan-based diagnostic model	1607:1641	CONCLUSIONS Our study indicated that the N-glycan-based diagnostic model would be a new, valuable, and noninvasive alternative for diagnosing psoriasis.					
33507566	7	82	dep	peaks	961:965	arg1	3					967:967	3	967:967	3	967:967	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	82	dep	peaks	961:965	arg1	NG1A2F					980:985	NG1A2F	980:985	NG1A2F	980:985	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	82	dep	peaks	961:965	arg1	NG1A2F					969:974	NG1A2F	969:974	NG1A2F	969:974	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	82	dep	peaks	961:965	arg1	6					989:989	6	989:989	6	989:989	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	82	dep	peaks	961:965	arg1	7					1002:1002	7	1002:1002	7	1002:1002	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	82	dep	peaks	961:965	arg1	NA2FB					1004:1008	NA2FB	1004:1008	NA2FB	1004:1008	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	82	dep	peaks	961:965	arg1	4					978:978	4	978:978	4	978:978	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	7	82	dep	peaks	961:965	arg1	NA2F					991:994	NA2F	991:994	NA2F	991:994	RESULTS Compared with the healthy controls, the relative abundance of structures in peaks 5(NA2), 9(NA3Fb), 11(NA4), and 12(NA4Fb) was elevated (p < .05), while that in peaks 3(NG1A2F), 4(NG1A2F), 6(NA2F), and 7(NA2FB) was decreased (p < .05) in the psoriasis group.					
33507566	4	83	theme	psoriasis	506:514	arg1	41					517:518	41	517:518	41	517:518	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	4	83	theme	psoriasis	506:514	arg1	psoriasis					506:514	psoriasis	506:514	psoriasis	506:514	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
33507566	4	83	theme	psoriasis	506:514	arg1	state					497:501	the state	493:501	the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe	493:588	We used Psoriasis Area Severity Index (PASI) scores to evaluate the state of psoriasis, 41 of whom were divided into three subgroups: mild, moderate, and severe.					
31965157	2	0	theme	epithelial	626:635	arg1	monolayer					637:645	a mucus-containing epithelial monolayer	607:645	a mucus-containing epithelial monolayer with elements of the innate immune system	607:687	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	8	1	from	presence	1682:1689	arg1	aspects					1642:1648	aspects	1642:1648	aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome	1642:1725	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	4	2	theme	barrier	927:933	arg1	functions					935:943	human intestinal barrier functions	910:943	human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains	910:997	Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs.					
31965157	7	3	theme	co-culture	1607:1616	arg1	model					1618:1622	the gut-immune co-culture model	1592:1622	the gut-immune co-culture model	1592:1622	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	7	4	theme	system	1557:1562	arg1	activation					1564:1573	immune system activation	1550:1573	immune system activation	1550:1573	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	8	5	theme	gut-immune	1760:1769	arg1	model					1771:1775	the gut-immune model	1756:1775	the gut-immune model	1756:1775	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	3	6	theme	host-pathogen	751:763	arg1	functions					849:857	host epithelial barrier functions	825:857	host epithelial barrier functions	825:857	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
31965157	3	6	theme	host-pathogen	751:763	arg1	invasion					870:877	bacterial invasion	860:877	bacterial invasion	860:877	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
31965157	3	6	theme	host-pathogen	751:763	arg1	immunogenicity					883:896	immunogenicity	883:896	immunogenicity	883:896	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
31965157	3	6	theme	host-pathogen	751:763	arg1	interactions					765:776	host-pathogen interactions	751:776	host-pathogen interactions	751:776	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
31965157	6	7	from	activating	1358:1367	arg1	suppressive					1334:1344	suppressive	1334:1344	suppressive	1334:1344	Quantification of inflammatory signaling revealed that 11168ΔpglE differentially modulated inflammatory responses in different intestinal microenvironments, suppressive in some but activating in others.					
31965157	4	8	theme	human	910:914	arg1	functions					935:943	human intestinal barrier functions	910:943	human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains	910:997	Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs.					
31965157	8	9	link	N-linked	1710:1717	arg1	glycome					1719:1725	its N-linked glycome	1706:1725	its N-linked glycome	1706:1725	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	3	10	theme	N-linked	793:800	arg1	glycosylation					802:814	N-linked glycosylation	793:814	N-linked glycosylation	793:814	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
31965157	8	11	from	absence	1695:1701	arg1	aspects					1642:1648	aspects	1642:1648	aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome	1642:1725	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	4	12	theme	intestinal	916:925	arg1	functions					935:943	human intestinal barrier functions	910:943	human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains	910:997	Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs.					
31965157	4	13	theme	jejuni	973:978	arg1	strains					991:997	11168 C. jejuni (wildtype) strains	964:997	11168 C. jejuni (wildtype) strains	964:997	Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs.					
31965157	8	14	theme	C.	1854:1855	arg1	jejuni					1857:1862	C. jejuni	1854:1862	C. jejuni	1854:1862	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	2	15	theme	human	550:554	arg1	enterocytes					556:566	human enterocytes	550:566	human enterocytes	550:566	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	5	16	theme	intestinal	1132:1141	arg1	model					1143:1147	this intestinal model	1127:1147	this intestinal model	1127:1147	The glycosylation-impaired strain 11168 ΔpglE was 100-fold less capable of adhering to and invading this intestinal model in cell infectivity assays.					
31965157	7	17	theme	differential	1490:1501	arg1	composition					1503:1513	differential composition	1490:1513	differential composition	1490:1513	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	4	18	theme	11168	964:968	arg1	strains					991:997	11168 C. jejuni (wildtype) strains	964:997	11168 C. jejuni (wildtype) strains	964:997	Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs.					
31965157	2	19	theme	cells	582:586	arg1	inclusion					507:515	the inclusion	503:515	the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells	503:586	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	8	20	theme	interest	1893:1900	arg1	studies					1843:1849	further studies	1835:1849	further studies of C. jejuni	1835:1862	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	8	20	theme	interest	1893:1900	arg1	enteropathogens					1874:1888	other enteropathogens	1868:1888	other enteropathogens of interest	1868:1900	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	6	21	theme	different	1294:1302	arg1	microenvironments					1315:1331	different intestinal microenvironments	1294:1331	different intestinal microenvironments	1294:1331	Quantification of inflammatory signaling revealed that 11168ΔpglE differentially modulated inflammatory responses in different intestinal microenvironments, suppressive in some but activating in others.					
31965157	3	22	theme	epithelial	830:839	arg1	functions					849:857	host epithelial barrier functions	825:857	host epithelial barrier functions	825:857	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
31965157	1	23	theme	protein	329:335	arg1	pathway					351:357	the N-linked protein glycosylation pathway	316:357	the N-linked protein glycosylation pathway	316:357	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	1	24	theme	co-culture	157:166	arg1	model					168:172	An in vitro gut-immune co-culture model	134:172	An in vitro gut-immune co-culture model	134:172	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	0	25	theme	N-glycan-dependent	63:80	arg1	interactions					96:107	N-glycan-dependent host-pathogen interactions	63:107	N-glycan-dependent host-pathogen interactions of Campylobacter jejuni	63:131	Application of a gut-immune co-culture system for the study of N-glycan-dependent host-pathogen interactions of Campylobacter jejuni.					
31965157	1	26	theme	pathway	351:357	arg1	role					308:311	the role	304:311	the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity	304:395	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	2	27	theme	cells	543:547	arg1	inclusion					507:515	the inclusion	503:515	the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells	503:586	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	2	28	theme	immune	675:680	arg1	system					682:687	the innate immune system	664:687	the innate immune system	664:687	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	1	29	dep	in	137:138	arg1	vitro					140:144	vitro	140:144	vitro	140:144	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	3	30	theme	bacterial	860:868	arg1	invasion					870:877	bacterial invasion	860:877	bacterial invasion	860:877	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
31965157	0	31	theme	interactions	96:107	arg1	study					54:58	the study	50:58	the study of N-glycan-dependent host-pathogen interactions of Campylobacter jejuni	50:131	Application of a gut-immune co-culture system for the study of N-glycan-dependent host-pathogen interactions of Campylobacter jejuni.					
31965157	1	32	theme	Campylobacter	362:374	arg1	jejuni					376:381	Campylobacter jejuni	362:381	Campylobacter jejuni pathogenicity	362:395	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	2	33	theme	mucin-producing	520:534	arg1	cells					543:547	mucin-producing goblet cells	520:547	mucin-producing goblet cells	520:547	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	0	34	theme	Campylobacter	112:124	arg1	jejuni					126:131	Campylobacter jejuni	112:131	Campylobacter jejuni	112:131	Application of a gut-immune co-culture system for the study of N-glycan-dependent host-pathogen interactions of Campylobacter jejuni.					
31965157	4	35	from	Changes	899:905	arg1	functions					935:943	human intestinal barrier functions	910:943	human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains	910:997	Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs.					
31965157	8	36	from	infectivity	1663:1673	arg1	presence					1682:1689	presence	1682:1689	presence	1682:1689	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	8	36	from	infectivity	1663:1673	arg1	absence					1695:1701	absence	1695:1701	absence	1695:1701	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	8	37	theme	jejuni	1656:1661	arg1	infectivity					1663:1673	C. jejuni infectivity	1653:1673	C. jejuni infectivity in the presence and absence of its N-linked glycome	1653:1725	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	2	38	theme	gut-immune	402:411	arg1	co-culture					413:422	The gut-immune co-culture	398:422	The gut-immune co-culture (GIC)	398:428	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	2	38	theme	gut-immune	402:411	arg1	GIC					425:427	GIC	425:427	GIC	425:427	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	7	39	theme	11168ΔpglE	1450:1459	arg1	jejuni					1464:1469	wildtype and 11168ΔpglE C. jejuni	1437:1469	wildtype and 11168ΔpglE C. jejuni	1437:1469	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	7	40	theme	wildtype	1437:1444	arg1	jejuni					1464:1469	wildtype and 11168ΔpglE C. jejuni	1437:1469	wildtype and 11168ΔpglE C. jejuni	1437:1469	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	2	41	theme	small	484:488	arg1	intestine					490:498	the human small intestine	474:498	the human small intestine	474:498	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	0	42	theme	co-culture	28:37	arg1	system					39:44	a gut-immune co-culture system	15:44	a gut-immune co-culture system	15:44	Application of a gut-immune co-culture system for the study of N-glycan-dependent host-pathogen interactions of Campylobacter jejuni.					
31965157	1	43	link	N-linked	320:327	arg1	pathway					351:357	the N-linked protein glycosylation pathway	316:357	the N-linked protein glycosylation pathway	316:357	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	7	44	theme	membrane	1407:1414	arg1	vesicles					1416:1423	Virulence-associated outer membrane vesicles	1380:1423	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni	1380:1469	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	8	45	theme	model	1771:1775	arg1	application					1741:1751	application	1741:1751	application of the gut-immune model	1741:1775	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	5	46	theme	strain	1054:1059	arg1	11168					1061:1065	The glycosylation-impaired strain 11168	1027:1065	The glycosylation-impaired strain 11168 ΔpglE	1027:1071	The glycosylation-impaired strain 11168 ΔpglE was 100-fold less capable of adhering to and invading this intestinal model in cell infectivity assays.					
31965157	7	47	theme	Virulence-associated	1380:1399	arg1	vesicles					1416:1423	Virulence-associated outer membrane vesicles	1380:1423	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni	1380:1469	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	8	48	theme	glycome	1719:1725	arg1	presence					1682:1689	presence	1682:1689	presence	1682:1689	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	8	48	theme	glycome	1719:1725	arg1	absence					1695:1701	absence	1695:1701	absence	1695:1701	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	7	49	contain	have	1485:1488	arg2	function					1519:1526	function	1519:1526	function	1519:1526	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	7	49	contain	have	1485:1488	arg1	vesicles					1416:1423	Virulence-associated outer membrane vesicles	1380:1423	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni	1380:1469	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	7	49	contain	have	1485:1488	arg2	composition					1503:1513	differential composition	1490:1513	differential composition	1490:1513	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	1	50	theme	intestinal	253:262	arg1	microenvironment					264:279	a human intestinal microenvironment	245:279	a human intestinal microenvironment	245:279	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	4	51	from	functions	935:943	arg1	presence					952:959	the presence	948:959	the presence of 11168 C. jejuni (wildtype) strains	948:997	Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs.					
31965157	7	52	theme	gut-immune	1596:1605	arg1	model					1618:1622	the gut-immune co-culture model	1592:1622	the gut-immune co-culture model	1592:1622	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	2	53	theme	mucus-containing	609:624	arg1	monolayer					637:645	a mucus-containing epithelial monolayer	607:645	a mucus-containing epithelial monolayer with elements of the innate immune system	607:687	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	4	54	theme	C.	970:971	arg1	strains					991:997	11168 C. jejuni (wildtype) strains	964:997	11168 C. jejuni (wildtype) strains	964:997	Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs.					
31965157	3	55	theme	system	709:714	arg1	utility					694:700	The utility	690:700	The utility of the system	690:714	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
31965157	1	56	from	role	308:311	arg1	pathogenicity					383:395	Campylobacter jejuni pathogenicity	362:395	Campylobacter jejuni pathogenicity	362:395	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	6	57	theme	inflammatory	1268:1279	arg1	responses					1281:1289	inflammatory responses	1268:1289	inflammatory responses	1268:1289	Quantification of inflammatory signaling revealed that 11168ΔpglE differentially modulated inflammatory responses in different intestinal microenvironments, suppressive in some but activating in others.					
31965157	7	58	theme	outer	1401:1405	arg1	vesicles					1416:1423	Virulence-associated outer membrane vesicles	1380:1423	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni	1380:1469	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	1	59	theme	apical	179:184	arg1	accessibility					196:208	apical and basal accessibility	179:208	apical and basal accessibility	179:208	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	7	60	theme	immune	1550:1555	arg1	activation					1564:1573	immune system activation	1550:1573	immune system activation	1550:1573	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	1	61	theme	basal	190:194	arg1	accessibility					196:208	apical and basal accessibility	179:208	apical and basal accessibility	179:208	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	2	62	with	monolayer	637:645	arg1	elements					652:659	elements	652:659	elements of the innate immune system	652:687	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	2	63	theme	enterocytes	556:566	arg1	inclusion					507:515	the inclusion	503:515	the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells	503:586	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	6	64	theme	signaling	1208:1216	arg1	Quantification					1177:1190	Quantification	1177:1190	Quantification of inflammatory signaling	1177:1216	Quantification of inflammatory signaling revealed that 11168ΔpglE differentially modulated inflammatory responses in different intestinal microenvironments, suppressive in some but activating in others.					
31965157	4	65	dep	strains	991:997	arg1	wildtype					981:988	wildtype	981:988	wildtype	981:988	Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs.					
31965157	4	66	theme	strains	991:997	arg1	presence					952:959	the presence	948:959	the presence of 11168 C. jejuni (wildtype) strains	948:997	Changes in human intestinal barrier functions in the presence of 11168 C. jejuni (wildtype) strains were quantified using GICs.					
31965157	8	67	theme	jejuni	1857:1862	arg1	studies					1843:1849	further studies	1835:1849	further studies of C. jejuni	1835:1862	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	8	67	theme	jejuni	1857:1862	arg1	enteropathogens					1874:1888	other enteropathogens	1868:1888	other enteropathogens of interest	1868:1900	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	6	68	from	suppressive	1334:1344	arg1	activating					1358:1367	activating	1358:1367	activating	1358:1367	Quantification of inflammatory signaling revealed that 11168ΔpglE differentially modulated inflammatory responses in different intestinal microenvironments, suppressive in some but activating in others.					
31965157	6	68	from	suppressive	1334:1344	arg1	some					1349:1352	some	1349:1352	some	1349:1352	Quantification of inflammatory signaling revealed that 11168ΔpglE differentially modulated inflammatory responses in different intestinal microenvironments, suppressive in some but activating in others.					
31965157	8	69	dep	presence	1682:1689	arg1	the					1678:1680	the	1678:1680	the	1678:1680	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	8	70	theme	other	1868:1872	arg1	enteropathogens					1874:1888	other enteropathogens	1868:1888	other enteropathogens of interest	1868:1900	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	1	71	theme	in	137:138	arg1	model					168:172	An in vitro gut-immune co-culture model	134:172	An in vitro gut-immune co-culture model	134:172	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	2	72	theme	dendritic	572:580	arg1	cells					582:586	dendritic cells	572:586	dendritic cells	572:586	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	6	73	theme	intestinal	1304:1313	arg1	microenvironments					1315:1331	different intestinal microenvironments	1294:1331	different intestinal microenvironments	1294:1331	Quantification of inflammatory signaling revealed that 11168ΔpglE differentially modulated inflammatory responses in different intestinal microenvironments, suppressive in some but activating in others.					
31965157	8	74	theme	further	1835:1841	arg1	studies					1843:1849	further studies	1835:1849	further studies of C. jejuni	1835:1862	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	6	75	from	some	1349:1352	arg1	suppressive					1334:1344	suppressive	1334:1344	suppressive	1334:1344	Quantification of inflammatory signaling revealed that 11168ΔpglE differentially modulated inflammatory responses in different intestinal microenvironments, suppressive in some but activating in others.					
31965157	1	76	theme	gut-immune	146:155	arg1	model					168:172	An in vitro gut-immune co-culture model	134:172	An in vitro gut-immune co-culture model	134:172	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	3	77	link	N-linked	793:800	arg1	glycosylation					802:814	N-linked glycosylation	793:814	N-linked glycosylation	793:814	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
31965157	3	78	theme	barrier	841:847	arg1	functions					849:857	host epithelial barrier functions	825:857	host epithelial barrier functions	825:857	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
31965157	0	79	theme	host-pathogen	82:94	arg1	interactions					96:107	N-glycan-dependent host-pathogen interactions	63:107	N-glycan-dependent host-pathogen interactions of Campylobacter jejuni	63:131	Application of a gut-immune co-culture system for the study of N-glycan-dependent host-pathogen interactions of Campylobacter jejuni.					
31965157	1	80	theme	glycosylation	337:349	arg1	pathway					351:357	the N-linked protein glycosylation pathway	316:357	the N-linked protein glycosylation pathway	316:357	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	1	81	with	model	168:172	arg1	accessibility					196:208	apical and basal accessibility	179:208	apical and basal accessibility	179:208	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	2	82	theme	intestine	490:498	arg1	aspects					463:469	important aspects	453:469	important aspects of the human small intestine	453:498	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	8	83	theme	aspects	1642:1648	arg1	analysis					1630:1637	This analysis	1625:1637	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome	1625:1725	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	2	84	theme	system	682:687	arg1	elements					652:659	elements	652:659	elements of the innate immune system	652:687	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	2	85	theme	goblet	536:541	arg1	cells					543:547	mucin-producing goblet cells	520:547	mucin-producing goblet cells	520:547	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	8	86	theme	C.	1653:1654	arg1	jejuni					1656:1661	C. jejuni	1653:1661	C. jejuni infectivity in the presence and absence of its N-linked glycome	1653:1725	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	2	87	theme	innate	668:673	arg1	system					682:687	the innate immune system	664:687	the innate immune system	664:687	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	0	88	theme	jejuni	126:131	arg1	interactions					96:107	N-glycan-dependent host-pathogen interactions	63:107	N-glycan-dependent host-pathogen interactions of Campylobacter jejuni	63:131	Application of a gut-immune co-culture system for the study of N-glycan-dependent host-pathogen interactions of Campylobacter jejuni.					
31965157	1	89	theme	jejuni	376:381	arg1	pathogenicity					383:395	Campylobacter jejuni pathogenicity	362:395	Campylobacter jejuni pathogenicity	362:395	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	7	90	theme	C.	1461:1462	arg1	jejuni					1464:1469	wildtype and 11168ΔpglE C. jejuni	1437:1469	wildtype and 11168ΔpglE C. jejuni	1437:1469	Virulence-associated outer membrane vesicles produced by wildtype and 11168ΔpglE C. jejuni were shown to have differential composition and function, with both leading to immune system activation when provided to the gut-immune co-culture model.					
31965157	8	91	theme	infectivity	1663:1673	arg1	aspects					1642:1648	aspects	1642:1648	aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome	1642:1725	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	8	92	from	aspects	1642:1648	arg1	presence					1682:1689	presence	1682:1689	presence	1682:1689	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	8	92	from	aspects	1642:1648	arg1	absence					1695:1701	absence	1695:1701	absence	1695:1701	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	5	93	theme	cell	1152:1155	arg1	assays					1169:1174	cell infectivity assays	1152:1174	cell infectivity assays	1152:1174	The glycosylation-impaired strain 11168 ΔpglE was 100-fold less capable of adhering to and invading this intestinal model in cell infectivity assays.					
31965157	2	94	theme	important	453:461	arg1	aspects					463:469	important aspects	453:469	important aspects of the human small intestine	453:498	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	0	95	theme	gut-immune	17:26	arg1	system					39:44	a gut-immune co-culture system	15:44	a gut-immune co-culture system	15:44	Application of a gut-immune co-culture system for the study of N-glycan-dependent host-pathogen interactions of Campylobacter jejuni.					
31965157	0	96	theme	system	39:44	arg1	Application					0:10	Application	0:10	Application of a gut-immune co-culture system for the study of N-glycan-dependent host-pathogen interactions of Campylobacter jejuni.	0:132	Application of a gut-immune co-culture system for the study of N-glycan-dependent host-pathogen interactions of Campylobacter jejuni.					
31965157	2	97	theme	human	478:482	arg1	intestine					490:498	the human small intestine	474:498	the human small intestine	474:498	The gut-immune co-culture (GIC) was developed to model important aspects of the human small intestine by the inclusion of mucin-producing goblet cells, human enterocytes and dendritic cells, bringing together a mucus-containing epithelial monolayer with elements of the innate immune system.					
31965157	5	98	theme	infectivity	1157:1167	arg1	assays					1169:1174	cell infectivity assays	1152:1174	cell infectivity assays	1152:1174	The glycosylation-impaired strain 11168 ΔpglE was 100-fold less capable of adhering to and invading this intestinal model in cell infectivity assays.					
31965157	6	99	theme	inflammatory	1195:1206	arg1	signaling					1208:1216	inflammatory signaling	1195:1216	inflammatory signaling	1195:1216	Quantification of inflammatory signaling revealed that 11168ΔpglE differentially modulated inflammatory responses in different intestinal microenvironments, suppressive in some but activating in others.					
31965157	8	100	theme	N-linked	1710:1717	arg1	glycome					1719:1725	its N-linked glycome	1706:1725	its N-linked glycome	1706:1725	This analysis of aspects of C. jejuni infectivity in the presence and absence of its N-linked glycome is enabled by application of the gut-immune model, and we anticipate that this system will be applicable to further studies of C. jejuni and other enteropathogens of interest.					
31965157	1	101	theme	N-linked	320:327	arg1	pathway					351:357	the N-linked protein glycosylation pathway	316:357	the N-linked protein glycosylation pathway	316:357	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	5	102	theme	glycosylation-impaired	1031:1052	arg1	11168					1061:1065	The glycosylation-impaired strain 11168	1027:1065	The glycosylation-impaired strain 11168 ΔpglE	1027:1071	The glycosylation-impaired strain 11168 ΔpglE was 100-fold less capable of adhering to and invading this intestinal model in cell infectivity assays.					
31965157	5	103	theme	11168	1061:1065	arg1	ΔpglE					1067:1071	The glycosylation-impaired strain 11168 ΔpglE	1027:1071	The glycosylation-impaired strain 11168 ΔpglE	1027:1071	The glycosylation-impaired strain 11168 ΔpglE was 100-fold less capable of adhering to and invading this intestinal model in cell infectivity assays.					
31965157	1	104	theme	human	247:251	arg1	microenvironment					264:279	a human intestinal microenvironment	245:279	a human intestinal microenvironment	245:279	An in vitro gut-immune co-culture model with apical and basal accessibility, designed to more closely resemble a human intestinal microenvironment, was employed to study the role of the N-linked protein glycosylation pathway in Campylobacter jejuni pathogenicity.					
31965157	3	105	theme	host	825:828	arg1	functions					849:857	host epithelial barrier functions	825:857	host epithelial barrier functions	825:857	The utility of the system was demonstrated by characterizing host-pathogen interactions facilitated by N-linked glycosylation, such as host epithelial barrier functions, bacterial invasion and immunogenicity.					
34541824	2	0	theme	active	449:454	arg1	complexes					466:474	active signaling complexes	449:474	active signaling complexes	449:474	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	2	1	theme	morphogens	330:339	arg1	range					290:294	a range	288:294	a range of growth factors (GFs) and other morphogens	288:339	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	0	2	theme	Glycan	74:79	arg1	Microenvironments					81:97	Engineered Glycan Microenvironments	63:97	Engineered Glycan Microenvironments	63:97	Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.					
34541824	5	3	theme	sulfate	894:900	arg1	polysaccharides					911:925	chemically modified heparan sulfate (HS) GAG polysaccharides	866:925	chemically modified heparan sulfate (HS) GAG polysaccharides	866:925	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	2	4	theme	other	324:328	arg1	morphogens					330:339	other morphogens	324:339	other morphogens	324:339	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	5	5	theme	gelatin	947:953	arg1	matrix					955:960	a gelatin matrix	945:960	a gelatin matrix	945:960	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	6	6	theme	kinase	1142:1147	arg1	pathway					1156:1162	the FGF2-dependent mitogen activated protein kinase (MAPK) pathway	1097:1162	the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells	1097:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	4	7	theme	establishing	727:738	arg1	relationships					759:771	establishing structure-function relationships	727:771	establishing structure-function relationships for this class of glycans	727:797	However, their structural complexity and compositional heterogeneity make establishing structure-function relationships for this class of glycans difficult.					
34541824	0	8	from	Signaling	50:58	arg1	Microenvironments					81:97	Engineered Glycan Microenvironments	63:97	Engineered Glycan Microenvironments	63:97	Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.					
34541824	6	9	theme	mouse	1167:1171	arg1	cells					1188:1192	mouse embryonic stem cells	1167:1192	mouse embryonic stem cells	1167:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	7	10	theme	synthetic	1233:1241	arg1	technologies					1259:1270	powerful synthetic and recombinant technologies	1224:1270	powerful synthetic and recombinant technologies	1224:1270	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	4	11	theme	structural	668:677	arg1	complexity					679:688	their structural complexity	662:688	their structural complexity	662:688	However, their structural complexity and compositional heterogeneity make establishing structure-function relationships for this class of glycans difficult.					
34541824	6	12	theme	FGF2-dependent	1101:1114	arg1	MAPK					1150:1153	MAPK	1150:1153	MAPK	1150:1153	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	6	12	theme	FGF2-dependent	1101:1114	arg1	kinase					1142:1147	the FGF2-dependent mitogen activated protein kinase	1097:1147	the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells	1097:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	1	13	dep	such	123:126	arg1	as					128:129	as	128:129	as	128:129	Extracellular glycans, such as glycosaminoglycans (GAGs), provide an essential regulatory component during the development and maintenance of tissues.					
34541824	6	14	theme	HS	1059:1060	arg1	contributions					1062:1074	HS contributions	1059:1074	HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells	1059:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	7	15	theme	powerful	1443:1450	arg1	tool					1452:1455	a powerful tool	1441:1455	a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications	1441:1553	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	2	16	theme	binding	270:276	arg1	sites					278:282	binding sites	270:282	binding sites for a range of growth factors (GFs) and other morphogens	270:339	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	1	17	theme	essential	169:177	arg1	component					190:198	an essential regulatory component	166:198	an essential regulatory component during the development and maintenance of tissues	166:248	Extracellular glycans, such as glycosaminoglycans (GAGs), provide an essential regulatory component during the development and maintenance of tissues.					
34541824	0	18	theme	Cell	5:8	arg1	Microarrays					10:20	Stem Cell Microarrays	0:20	Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.	0:98	Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.					
34541824	7	19	theme	recent	1204:1209	arg1	emergence					1211:1219	the recent emergence	1200:1219	the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures	1200:1309	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	0	20	theme	Engineered	63:72	arg1	Microenvironments					81:97	Engineered Glycan Microenvironments	63:97	Engineered Glycan Microenvironments	63:97	Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.					
34541824	2	21	theme	factors	306:312	arg1	range					290:294	a range	288:294	a range of growth factors (GFs) and other morphogens	288:339	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	1	22	theme	regulatory	179:188	arg1	component					190:198	an essential regulatory component	166:198	an essential regulatory component during the development and maintenance of tissues	166:248	Extracellular glycans, such as glycosaminoglycans (GAGs), provide an essential regulatory component during the development and maintenance of tissues.					
34541824	0	23	theme	Stem	0:3	arg1	Microarrays					10:20	Stem Cell Microarrays	0:20	Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.	0:98	Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.					
34541824	7	24	theme	powerful	1224:1231	arg1	technologies					1259:1270	powerful synthetic and recombinant technologies	1224:1270	powerful synthetic and recombinant technologies	1224:1270	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	1	25	theme	Extracellular	100:112	arg1	glycans					114:120	Extracellular glycans	100:120	Extracellular glycans	100:120	Extracellular glycans, such as glycosaminoglycans (GAGs), provide an essential regulatory component during the development and maintenance of tissues.					
34541824	5	26	theme	stem	818:821	arg1	platform					834:841	a stem cell array platform	816:841	a stem cell array platform	816:841	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	5	27	theme	polyacrylamide	984:997	arg1	network					1008:1014	a polyacrylamide hydrogel network	982:1014	a polyacrylamide hydrogel network	982:1014	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	7	28	theme	factor	1349:1354	arg1	binding					1356:1362	growth factor binding	1342:1362	growth factor binding	1342:1362	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	2	29	theme	extracellular	393:405	arg1	ECM					415:417	ECM	415:417	ECM	415:417	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	2	29	theme	extracellular	393:405	arg1	matrix					407:412	the extracellular matrix	389:412	the extracellular matrix (ECM)	389:418	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	2	30	theme	growth	299:304	arg1	GFs					315:317	GFs	315:317	GFs	315:317	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	2	30	theme	growth	299:304	arg1	factors					306:312	growth factors	299:312	growth factors (GFs)	299:318	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	6	31	theme	embryonic	1173:1181	arg1	cells					1188:1192	mouse embryonic stem cells	1167:1192	mouse embryonic stem cells	1167:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	7	32	theme	biomolecules	1415:1426	arg1	presence					1397:1404	the presence	1393:1404	the presence of these biomolecules	1393:1426	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	3	33	theme	biomaterials	601:612	arg1	development					586:596	the development	582:596	the development of biomaterials for cell-based regenerative therapies	582:650	As such, GAGs have been pursued as biologically active components for the development of biomaterials for cell-based regenerative therapies.					
34541824	4	34	theme	structure-function	740:757	arg1	relationships					759:771	establishing structure-function relationships	727:771	establishing structure-function relationships for this class of glycans	727:797	However, their structural complexity and compositional heterogeneity make establishing structure-function relationships for this class of glycans difficult.					
34541824	6	35	theme	direct	1040:1045	arg1	analysis					1047:1054	direct analysis	1040:1054	direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells	1040:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	0	36	theme	Growth	36:41	arg1	Signaling					50:58	Growth Factor Signaling	36:58	Growth Factor Signaling in Engineered Glycan Microenvironments	36:97	Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.					
34541824	6	37	from	pathway	1156:1162	arg1	cells					1188:1192	mouse embryonic stem cells	1167:1192	mouse embryonic stem cells	1167:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	7	38	theme	GAG	1296:1298	arg1	structures					1300:1309	well-defined GAG structures	1283:1309	well-defined GAG structures	1283:1309	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	2	39	theme	cell	498:501	arg1	surface					503:509	the cell surface	494:509	the cell surface	494:509	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	2	40	theme	molecules	376:384	arg1	gradients					357:365	gradients	357:365	gradients of these molecules	357:384	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	5	41	theme	cell	823:826	arg1	platform					834:841	a stem cell array platform	816:841	a stem cell array platform	816:841	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	7	42	theme	growth	1342:1347	arg1	binding					1356:1362	growth factor binding	1342:1362	growth factor binding	1342:1362	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	6	43	theme	protein	1134:1140	arg1	MAPK					1150:1153	MAPK	1150:1153	MAPK	1150:1153	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	6	43	theme	protein	1134:1140	arg1	kinase					1142:1147	the FGF2-dependent mitogen activated protein kinase	1097:1147	the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells	1097:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	0	44	theme	Factor	43:48	arg1	Signaling					50:58	Growth Factor Signaling	36:58	Growth Factor Signaling in Engineered Glycan Microenvironments	36:97	Stem Cell Microarrays for Assessing Growth Factor Signaling in Engineered Glycan Microenvironments.					
34541824	7	45	dep	presence	1397:1404	arg1	response					1381:1388	response	1381:1388	response	1381:1388	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	7	46	theme	biological	1516:1525	arg1	properties					1527:1536	their biological properties	1510:1536	their biological properties	1510:1536	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	6	47	theme	activated	1124:1132	arg1	MAPK					1150:1153	MAPK	1150:1153	MAPK	1150:1153	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	6	47	theme	activated	1124:1132	arg1	kinase					1142:1147	the FGF2-dependent mitogen activated protein kinase	1097:1147	the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells	1097:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	6	48	theme	stem	1183:1186	arg1	cells					1188:1192	mouse embryonic stem cells	1167:1192	mouse embryonic stem cells	1167:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	1	49	dep	development	211:221	arg1	the					207:209	the	207:209	the	207:209	Extracellular glycans, such as glycosaminoglycans (GAGs), provide an essential regulatory component during the development and maintenance of tissues.					
34541824	7	50	theme	recombinant	1247:1257	arg1	technologies					1259:1270	powerful synthetic and recombinant technologies	1224:1270	powerful synthetic and recombinant technologies	1224:1270	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	5	51	theme	GAG	907:909	arg1	polysaccharides					911:925	chemically modified heparan sulfate (HS) GAG polysaccharides	866:925	chemically modified heparan sulfate (HS) GAG polysaccharides	866:925	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	3	52	theme	cell-based	618:627	arg1	therapies					642:650	cell-based regenerative therapies	618:650	cell-based regenerative therapies	618:650	As such, GAGs have been pursued as biologically active components for the development of biomaterials for cell-based regenerative therapies.					
34541824	4	53	theme	glycans	791:797	arg1	class					782:786	this class	777:786	this class of glycans	777:797	However, their structural complexity and compositional heterogeneity make establishing structure-function relationships for this class of glycans difficult.					
34541824	4	54	theme	compositional	694:706	arg1	heterogeneity					708:720	compositional heterogeneity	694:720	compositional heterogeneity	694:720	However, their structural complexity and compositional heterogeneity make establishing structure-function relationships for this class of glycans difficult.					
34541824	6	55	theme	mitogen	1116:1122	arg1	MAPK					1150:1153	MAPK	1150:1153	MAPK	1150:1153	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	6	55	theme	mitogen	1116:1122	arg1	kinase					1142:1147	the FGF2-dependent mitogen activated protein kinase	1097:1147	the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells	1097:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	2	56	theme	complexes	466:474	arg1	formation					436:444	the formation	432:444	the formation of active signaling complexes	432:474	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	3	57	theme	regenerative	629:640	arg1	therapies					642:650	cell-based regenerative therapies	618:650	cell-based regenerative therapies	618:650	As such, GAGs have been pursued as biologically active components for the development of biomaterials for cell-based regenerative therapies.					
34541824	3	58	theme	active	560:565	arg1	components					567:576	biologically active components	547:576	biologically active components for the development of biomaterials for cell-based regenerative therapies	547:650	As such, GAGs have been pursued as biologically active components for the development of biomaterials for cell-based regenerative therapies.					
34541824	3	58	theme	active	560:565	arg1	GAGs					521:524	GAGs	521:524	GAGs	521:524	As such, GAGs have been pursued as biologically active components for the development of biomaterials for cell-based regenerative therapies.					
34541824	5	59	theme	array	828:832	arg1	platform					834:841	a stem cell array platform	816:841	a stem cell array platform	816:841	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	5	60	theme	modified	877:884	arg1	polysaccharides					911:925	chemically modified heparan sulfate (HS) GAG polysaccharides	866:925	chemically modified heparan sulfate (HS) GAG polysaccharides	866:925	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	6	61	theme	contributions	1062:1074	arg1	analysis					1047:1054	direct analysis	1040:1054	direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells	1040:1192	This array allowed for direct analysis of HS contributions to the signaling via the FGF2-dependent mitogen activated protein kinase (MAPK) pathway in mouse embryonic stem cells.					
34541824	1	62	theme	tissues	242:248	arg1	maintenance					227:237	maintenance	227:237	maintenance	227:237	Extracellular glycans, such as glycosaminoglycans (GAGs), provide an essential regulatory component during the development and maintenance of tissues.					
34541824	1	62	theme	tissues	242:248	arg1	development					211:221	development	211:221	development	211:221	Extracellular glycans, such as glycosaminoglycans (GAGs), provide an essential regulatory component during the development and maintenance of tissues.					
34541824	2	63	theme	signaling	456:464	arg1	complexes					466:474	active signaling complexes	449:474	active signaling complexes	449:474	GAGs, which harbor binding sites for a range of growth factors (GFs) and other morphogens, help establish gradients of these molecules in the extracellular matrix (ECM) and promote the formation of active signaling complexes when presented at the cell surface.					
34541824	5	64	theme	hydrogel	999:1006	arg1	network					1008:1014	a polyacrylamide hydrogel network	982:1014	a polyacrylamide hydrogel network	982:1014	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	7	65	theme	technologies	1259:1270	arg1	emergence					1211:1219	the recent emergence	1200:1219	the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures	1200:1309	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34541824	5	66	theme	heparan	886:892	arg1	sulfate					894:900	heparan sulfate	886:900	chemically modified heparan sulfate (HS) GAG polysaccharides	866:925	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	5	66	theme	heparan	886:892	arg1	HS					903:904	HS	903:904	HS	903:904	Here, a stem cell array platform is described, in which chemically modified heparan sulfate (HS) GAG polysaccharides are conjugated to a gelatin matrix and introduced into a polyacrylamide hydrogel network.					
34541824	7	67	theme	well-defined	1283:1294	arg1	structures					1300:1309	well-defined GAG structures	1283:1309	well-defined GAG structures	1283:1309	With the recent emergence of powerful synthetic and recombinant technologies to produce well-defined GAG structures, a platform for analyzing both growth factor binding and signaling in response to the presence of these biomolecules will provide a powerful tool for integrating glycans into biomaterials to advance their biological properties and applications.					
34440649	4	0	theme	distribution	880:891	arg1	pattern					869:875	a specific persistent spatio-temporal pattern	831:875	a specific persistent spatio-temporal pattern of distribution	831:891	In typical conditions, AGPs are characterized by a specific persistent spatio-temporal pattern of distribution.					
34440649	7	1	attach	present	1210:1216	arg1	microspores					1255:1265	microspores	1255:1265	microspores	1255:1265	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	1	attach	present	1210:1216	arg2	AGPs					1201:1204	AGPs	1201:1204	AGPs	1201:1204	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	1	attach	present	1210:1216	arg1	case					1175:1178	the case	1171:1178	the case of male development	1171:1198	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	1	attach	present	1210:1216	arg1	microsporocytes					1234:1248	microsporocytes	1234:1248	microsporocytes	1234:1248	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	1	attach	present	1210:1216	arg1	tapetum					1225:1231	tapetum	1225:1231	tapetum	1225:1231	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	2	from	case	1175:1178	arg1	present					1210:1216	present	1210:1216	present	1210:1216	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	8	3	from	temperature	1325:1335	arg1	development					1304:1314	development	1304:1314	development at lower temperature	1304:1335	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	1	4	theme	cell	233:236	arg1	continuum					259:267	the cell wall-plasma membrane continuum	229:267	the cell wall-plasma membrane continuum	229:267	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	1	5	theme	membrane	250:257	arg1	continuum					259:267	the cell wall-plasma membrane continuum	229:267	the cell wall-plasma membrane continuum	229:267	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	4	6	theme	persistent	842:851	arg1	pattern					869:875	a specific persistent spatio-temporal pattern	831:875	a specific persistent spatio-temporal pattern of distribution	831:891	In typical conditions, AGPs are characterized by a specific persistent spatio-temporal pattern of distribution.					
34440649	6	7	theme	embryo	1151:1156	arg1	wall					1162:1165	the embryo sac wall	1147:1165	the embryo sac wall	1147:1165	During development in stress conditions, the AGP localization is altered, and AGPs entirely disappear in the embryo sac wall.					
34440649	3	8	from	changes	651:657	arg1	structures					715:724	female and male reproductive structures	686:724	female and male reproductive structures	686:724	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	3	8	from	changes	651:657	arg1	distribution					670:681	the AGP distribution	662:681	the AGP distribution	662:681	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	3	9	theme	reproductive	702:713	arg1	structures					715:724	female and male reproductive structures	686:724	female and male reproductive structures	686:724	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	1	10	gly	glycosylated	170:181	arg1	proteins					183:190	heavily glycosylated proteins	162:190	heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum	162:267	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	5	11	from	megaspores	986:995	arg1	visible					911:917	visible	911:917	visible	911:917	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	7	12	from	tapetum	1225:1231	arg1	present					1210:1216	present	1210:1216	present	1210:1216	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	12	from	tapetum	1225:1231	arg1	conditions					1277:1286	normal conditions	1270:1286	normal conditions	1270:1286	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	5	13	theme	AGP	894:896	arg1	epitopes					898:905	AGP epitopes	894:905	AGP epitopes	894:905	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	7	14	from	microsporocytes	1234:1248	arg1	present					1210:1216	present	1210:1216	present	1210:1216	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	14	from	microsporocytes	1234:1248	arg1	conditions					1277:1286	normal conditions	1270:1286	normal conditions	1270:1286	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	3	15	theme	first	737:741	arg1	stages					743:748	the first stages	733:748	the first stages of Bellis perennis development	733:779	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	0	16	theme	Anthers	106:112	arg1	Development					64:74	Development	64:74	Development of Bellis perennis Ovules and Anthers	64:112	Effect of Low Temperature on Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers.					
34440649	8	17	from	wall	1371:1374	arg1	localized					1347:1355	localized	1347:1355	localized	1347:1355	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	1	18	theme	proteins	183:190	arg1	proteins					131:138	Arabinogalactan proteins	115:138	Arabinogalactan proteins (AGPs)	115:145	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	1	18	theme	proteins	183:190	arg1	class					153:157	a class	151:157	a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum	151:267	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	8	19	theme	mature	1398:1403	arg1	grains					1412:1417	mature pollen grains	1398:1417	mature pollen grains	1398:1417	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	3	20	theme	perennis	760:767	arg1	development					769:779	Bellis perennis development	753:779	Bellis perennis development	753:779	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	5	21	theme	cell	926:929	arg1	walls					931:935	the cell walls	922:935	the cell walls of somatic cells	922:952	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	3	22	dep	antibodies	554:563	arg1	JIM13					566:570	JIM13	566:570	JIM13	566:570	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	3	23	theme	carbohydrate	604:615	arg1	chains					617:622	the carbohydrate chains	600:622	the carbohydrate chains of AGPs	600:630	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	7	24	theme	male	1183:1186	arg1	development					1188:1198	male development	1183:1198	male development	1183:1198	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	5	25	from	visible	911:917	arg1	walls					979:983	the megasporocyte walls	961:983	the megasporocyte walls	961:983	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	5	25	from	visible	911:917	arg1	megaspores					986:995	megaspores	986:995	megaspores	986:995	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	5	25	from	visible	911:917	arg1	walls					931:935	the cell walls	922:935	the cell walls of somatic cells	922:952	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	5	25	from	visible	911:917	arg1	sac					1009:1011	embryo sac	1002:1011	embryo sac	1002:1011	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	9	26	theme	cells	1478:1482	arg1	remnants					1458:1465	remnants	1458:1465	remnants of tapetum cells	1458:1482	Additionally, they are accumulated in remnants of tapetum cells.					
34440649	0	27	from	Effect	0:5	arg1	Changes					29:35	Changes	29:35	Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers	29:112	Effect of Low Temperature on Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers.					
34440649	0	28	theme	Bellis	79:84	arg1	Ovules					95:100	Bellis perennis Ovules	79:100	Bellis perennis Ovules	79:100	Effect of Low Temperature on Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers.					
34440649	7	29	dep	tapetum	1225:1231	arg1	the					1221:1223	the	1221:1223	the	1221:1223	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	2	30	theme	stress	376:381	arg1	conditions					383:392	stress conditions	376:392	stress conditions	376:392	The features of AGPs described earlier suggest that the proteins may be implicated in plant adaptation to stress conditions in important developmental phases during the plant reproduction process.					
34440649	0	31	theme	Ovules	95:100	arg1	Development					64:74	Development	64:74	Development of Bellis perennis Ovules and Anthers	64:112	Effect of Low Temperature on Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers.					
34440649	5	32	from	walls	931:935	arg1	visible					911:917	visible	911:917	visible	911:917	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	6	33	theme	stress	1064:1069	arg1	conditions					1071:1080	stress conditions	1064:1080	stress conditions	1064:1080	During development in stress conditions, the AGP localization is altered, and AGPs entirely disappear in the embryo sac wall.					
34440649	2	34	theme	plant	439:443	arg1	process					458:464	the plant reproduction process	435:464	the plant reproduction process	435:464	The features of AGPs described earlier suggest that the proteins may be implicated in plant adaptation to stress conditions in important developmental phases during the plant reproduction process.					
34440649	1	35	theme	continuum	259:267	arg1	element					218:224	a structural element	205:224	a structural element of the cell wall-plasma membrane continuum	205:267	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	5	36	theme	cells	948:952	arg1	walls					931:935	the cell walls	922:935	the cell walls of somatic cells	922:952	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	2	37	theme	developmental	407:419	arg1	phases					421:426	important developmental phases	397:426	important developmental phases during the plant reproduction process	397:464	The features of AGPs described earlier suggest that the proteins may be implicated in plant adaptation to stress conditions in important developmental phases during the plant reproduction process.					
34440649	0	38	theme	Low	10:12	arg1	Temperature					14:24	Low Temperature	10:24	Low Temperature	10:24	Effect of Low Temperature on Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers.					
34440649	2	39	from	adaptation	362:371	arg1	phases					421:426	important developmental phases	397:426	important developmental phases during the plant reproduction process	397:464	The features of AGPs described earlier suggest that the proteins may be implicated in plant adaptation to stress conditions in important developmental phases during the plant reproduction process.					
34440649	7	40	from	microspores	1255:1265	arg1	present					1210:1216	present	1210:1216	present	1210:1216	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	40	from	microspores	1255:1265	arg1	conditions					1277:1286	normal conditions	1270:1286	normal conditions	1270:1286	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	8	41	from	localized	1347:1355	arg1	wall					1371:1374	the common wall	1360:1374	the common wall of microspores	1360:1389	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	8	41	from	localized	1347:1355	arg1	grains					1412:1417	mature pollen grains	1398:1417	mature pollen grains	1398:1417	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	8	42	theme	microspores	1379:1389	arg1	wall					1371:1374	the common wall	1360:1374	the common wall of microspores	1360:1389	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	3	43	theme	immunocytochemical	502:519	arg1	studies					521:527	the microscopic and immunocytochemical studies	482:527	the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs	482:630	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	5	44	theme	embryo	1002:1007	arg1	sac					1009:1011	embryo sac	1002:1011	embryo sac	1002:1011	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	6	45	theme	AGP	1087:1089	arg1	localization					1091:1102	the AGP localization	1083:1102	the AGP localization	1083:1102	During development in stress conditions, the AGP localization is altered, and AGPs entirely disappear in the embryo sac wall.					
34440649	3	46	theme	male	697:700	arg1	structures					715:724	female and male reproductive structures	686:724	female and male reproductive structures	686:724	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	3	47	theme	microscopic	486:496	arg1	studies					521:527	the microscopic and immunocytochemical studies	482:527	the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs	482:630	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	3	48	theme	female	686:691	arg1	structures					715:724	female and male reproductive structures	686:724	female and male reproductive structures	686:724	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	6	49	from	development	1049:1059	arg1	conditions					1071:1080	stress conditions	1064:1080	stress conditions	1064:1080	During development in stress conditions, the AGP localization is altered, and AGPs entirely disappear in the embryo sac wall.					
34440649	3	50	theme	specific	545:552	arg1	antibodies					554:563	specific antibodies	545:563	specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs	545:630	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	1	51	theme	wall-plasma	238:248	arg1	continuum					259:267	the cell wall-plasma membrane continuum	229:267	the cell wall-plasma membrane continuum	229:267	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	7	52	from	present	1210:1216	arg1	microspores					1255:1265	microspores	1255:1265	microspores	1255:1265	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	52	from	present	1210:1216	arg1	tapetum					1225:1231	tapetum	1225:1231	tapetum	1225:1231	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	52	from	present	1210:1216	arg1	case					1175:1178	the case	1171:1178	the case of male development	1171:1198	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	52	from	present	1210:1216	arg1	microsporocytes					1234:1248	microsporocytes	1234:1248	microsporocytes	1234:1248	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	6	53	theme	sac	1158:1160	arg1	wall					1162:1165	the embryo sac wall	1147:1165	the embryo sac wall	1147:1165	During development in stress conditions, the AGP localization is altered, and AGPs entirely disappear in the embryo sac wall.					
34440649	4	54	theme	specific	833:840	arg1	pattern					869:875	a specific persistent spatio-temporal pattern	831:875	a specific persistent spatio-temporal pattern of distribution	831:891	In typical conditions, AGPs are characterized by a specific persistent spatio-temporal pattern of distribution.					
34440649	6	55	dep	AGPs	1120:1123	arg1	disappear					1134:1142	disappear	1134:1142	AGPs entirely disappear in the embryo sac wall	1120:1165	During development in stress conditions, the AGP localization is altered, and AGPs entirely disappear in the embryo sac wall.					
34440649	4	56	theme	spatio-temporal	853:867	arg1	pattern					869:875	a specific persistent spatio-temporal pattern	831:875	a specific persistent spatio-temporal pattern of distribution	831:891	In typical conditions, AGPs are characterized by a specific persistent spatio-temporal pattern of distribution.					
34440649	3	57	theme	Bellis	753:758	arg1	development					769:779	Bellis perennis development	753:779	Bellis perennis development	753:779	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	2	58	theme	AGPs	286:289	arg1	features					274:281	The features	270:281	The features of AGPs described earlier	270:307	The features of AGPs described earlier suggest that the proteins may be implicated in plant adaptation to stress conditions in important developmental phases during the plant reproduction process.					
34440649	5	59	from	stage	1022:1026	arg1	visible					911:917	visible	911:917	visible	911:917	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	1	60	theme	glycosylated	170:181	arg1	proteins					183:190	heavily glycosylated proteins	162:190	heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum	162:267	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	8	61	theme	pollen	1405:1410	arg1	grains					1412:1417	mature pollen grains	1398:1417	mature pollen grains	1398:1417	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	3	62	theme	development	769:779	arg1	stages					743:748	the first stages	733:748	the first stages of Bellis perennis development	733:779	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	5	63	theme	formation	1031:1039	arg1	stage					1022:1026	every stage	1016:1026	every stage of formation	1016:1039	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	1	64	theme	Arabinogalactan	115:129	arg1	AGPs					141:144	AGPs	141:144	AGPs	141:144	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	1	64	theme	Arabinogalactan	115:129	arg1	proteins					131:138	Arabinogalactan proteins	115:138	Arabinogalactan proteins (AGPs)	115:145	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	1	64	theme	Arabinogalactan	115:129	arg1	class					153:157	a class	151:157	a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum	151:267	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	7	65	theme	development	1188:1198	arg1	case					1175:1178	the case	1171:1178	the case of male development	1171:1198	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	66	located	present	1210:1216	arg1	microspores					1255:1265	microspores	1255:1265	microspores	1255:1265	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	66	located	present	1210:1216	arg2	AGPs					1201:1204	AGPs	1201:1204	AGPs	1201:1204	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	66	located	present	1210:1216	arg1	case					1175:1178	the case	1171:1178	the case of male development	1171:1198	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	66	located	present	1210:1216	arg1	microsporocytes					1234:1248	microsporocytes	1234:1248	microsporocytes	1234:1248	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	7	66	located	present	1210:1216	arg1	tapetum					1225:1231	tapetum	1225:1231	tapetum	1225:1231	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	9	67	theme	tapetum	1470:1476	arg1	cells					1478:1482	tapetum cells	1470:1482	tapetum cells	1470:1482	Additionally, they are accumulated in remnants of tapetum cells.					
34440649	0	68	theme	perennis	86:93	arg1	Ovules					95:100	Bellis perennis Ovules	79:100	Bellis perennis Ovules	79:100	Effect of Low Temperature on Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers.					
34440649	4	69	theme	typical	785:791	arg1	conditions					793:802	typical conditions	785:802	typical conditions	785:802	In typical conditions, AGPs are characterized by a specific persistent spatio-temporal pattern of distribution.					
34440649	5	70	theme	megasporocyte	965:977	arg1	walls					979:983	the megasporocyte walls	961:983	the megasporocyte walls	961:983	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	5	71	from	sac	1009:1011	arg1	visible					911:917	visible	911:917	visible	911:917	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	8	72	dep	development	1304:1314	arg1	response					1292:1299	response	1292:1299	response	1292:1299	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	0	73	from	Changes	29:35	arg1	Distribution					44:55	AGP Distribution	40:55	AGP Distribution	40:55	Effect of Low Temperature on Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers.					
34440649	5	74	theme	somatic	940:946	arg1	cells					948:952	somatic cells	940:952	somatic cells	940:952	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
34440649	7	75	theme	normal	1270:1275	arg1	conditions					1277:1286	normal conditions	1270:1286	normal conditions	1270:1286	In the case of male development, AGPs are present in the tapetum, microsporocytes, and microspores in normal conditions.					
34440649	8	76	theme	lower	1319:1323	arg1	temperature					1325:1335	lower temperature	1319:1335	lower temperature	1319:1335	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	2	77	theme	plant	356:360	arg1	adaptation					362:371	plant adaptation	356:371	plant adaptation to stress conditions in important developmental phases during the plant reproduction process	356:464	The features of AGPs described earlier suggest that the proteins may be implicated in plant adaptation to stress conditions in important developmental phases during the plant reproduction process.					
34440649	0	78	theme	Temperature	14:24	arg1	Effect					0:5	Effect	0:5	Effect of Low Temperature on Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers.	0:113	Effect of Low Temperature on Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers.					
34440649	3	79	theme	significant	639:649	arg1	changes					651:657	significant changes	639:657	significant changes in the AGP distribution in female and male reproductive structures	639:724	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	2	80	theme	important	397:405	arg1	phases					421:426	important developmental phases	397:426	important developmental phases during the plant reproduction process	397:464	The features of AGPs described earlier suggest that the proteins may be implicated in plant adaptation to stress conditions in important developmental phases during the plant reproduction process.					
34440649	3	81	theme	AGPs	627:630	arg1	chains					617:622	the carbohydrate chains	600:622	the carbohydrate chains of AGPs	600:630	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	8	82	from	grains	1412:1417	arg1	localized					1347:1355	localized	1347:1355	localized	1347:1355	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	0	83	theme	AGP	40:42	arg1	Distribution					44:55	AGP Distribution	40:55	AGP Distribution	40:55	Effect of Low Temperature on Changes in AGP Distribution during Development of Bellis perennis Ovules and Anthers.					
34440649	2	84	theme	reproduction	445:456	arg1	process					458:464	the plant reproduction process	435:464	the plant reproduction process	435:464	The features of AGPs described earlier suggest that the proteins may be implicated in plant adaptation to stress conditions in important developmental phases during the plant reproduction process.					
34440649	3	85	theme	AGP	666:668	arg1	distribution					670:681	the AGP distribution	662:681	the AGP distribution	662:681	In this paper, the microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing the carbohydrate chains of AGPs showed significant changes in the AGP distribution in female and male reproductive structures during the first stages of Bellis perennis development.					
34440649	8	86	theme	common	1364:1369	arg1	wall					1371:1374	the common wall	1360:1374	the common wall of microspores	1360:1389	In response to development at lower temperature, AGPs are localized in the common wall of microspores and in mature pollen grains.					
34440649	1	87	theme	structural	207:216	arg1	element					218:224	a structural element	205:224	a structural element of the cell wall-plasma membrane continuum	205:267	Arabinogalactan proteins (AGPs) are a class of heavily glycosylated proteins occurring as a structural element of the cell wall-plasma membrane continuum.					
34440649	5	88	from	walls	979:983	arg1	visible					911:917	visible	911:917	visible	911:917	AGP epitopes are visible in the cell walls of somatic cells and in the megasporocyte walls, megaspores, and embryo sac at every stage of formation.					
31907284	2	0	theme	different	438:446	arg1	systems					448:454	different systems	438:454	different systems	438:454	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	2	1	theme	IgG-Fc	579:584	arg1	profile					600:606	the final IgG-Fc glycosylation profile	569:606	the final IgG-Fc glycosylation profile	569:606	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	4	2	theme	Fc	840:841	arg1	mutants					843:849	Fc mutants	840:849	Fc mutants	840:849	We show that the types of N-linked glycans between matched pairs of Fc mutants vary greatly and in particular, with respect, to sialylation.					
31907284	3	3	theme	glycan-adapted	649:662	arg1	mutants					672:678	glycan-adapted IgG1-Fc mutants	649:678	glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems	649:769	In this study, we compare two panels of glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems.					
31907284	1	4	link	N-linked	182:189	arg1	carbohydrate					191:202	a conserved N-linked carbohydrate	170:202	a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes	170:351	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	4	5	theme	mutants	843:849	arg1	pairs					831:835	matched pairs	823:835	matched pairs of Fc mutants	823:849	We show that the types of N-linked glycans between matched pairs of Fc mutants vary greatly and in particular, with respect, to sialylation.					
31907284	2	6	theme	different	464:472	arg1	enzymes					488:494	different glycosylation enzymes	464:494	different glycosylation enzymes	464:494	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	0	7	theme	Fc	78:79	arg1	Region					81:86	the Fc Region	74:86	the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses	74:134	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	3	8	theme	IgG1-Fc	664:670	arg1	mutants					672:678	glycan-adapted IgG1-Fc mutants	649:678	glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems	649:769	In this study, we compare two panels of glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems.					
31907284	6	9	theme	endothelial	1240:1250	arg1	cells					1265:1269	human endothelial kidney 293-F cells	1234:1269	human endothelial kidney 293-F cells	1234:1269	For example, we describe Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells.					
31907284	6	10	theme	Chinese	1197:1203	arg1	ovary-K1					1213:1220	Chinese hamster ovary-K1	1197:1220	Chinese hamster ovary-K1	1197:1220	For example, we describe Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells.					
31907284	5	11	theme	cell	919:922	arg1	effects					929:935	These cell line effects	913:935	These cell line effects on glycosylation	913:952	These cell line effects on glycosylation profoundly influence the ability of the engineered Fcs to interact with either human or pathogen receptors.					
31907284	1	12	dep	in	285:286	arg1	vivo					288:291	vivo	288:291	vivo	288:291	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	1	13	gly	glycoproteins	145:157	arg1	glycoproteins					145:157	glycoproteins	145:157	glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes	145:351	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	1	13	gly	glycoproteins	145:157	arg1	Abs					137:139	Abs	137:139	Abs	137:139	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	6	14	theme	human	1234:1238	arg1	cells					1265:1269	human endothelial kidney 293-F cells	1234:1269	human endothelial kidney 293-F cells	1234:1269	For example, we describe Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells.					
31907284	5	15	theme	line	924:927	arg1	effects					929:935	These cell line effects	913:935	These cell line effects on glycosylation	913:952	These cell line effects on glycosylation profoundly influence the ability of the engineered Fcs to interact with either human or pathogen receptors.					
31907284	2	16	theme	glycosylation	474:486	arg1	enzymes					488:494	different glycosylation enzymes	464:494	different glycosylation enzymes	464:494	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	1	17	theme	functional	331:340	arg1	attributes					342:351	functional attributes	331:351	functional attributes	331:351	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	0	18	gly	Glycosylation	57:69	arg1	Region					81:86	the Fc Region	74:86	the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses	74:134	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	4	19	link	N-linked	798:805	arg1	glycans					807:813	N-linked glycans	798:813	N-linked glycans between matched pairs of Fc mutants	798:849	We show that the types of N-linked glycans between matched pairs of Fc mutants vary greatly and in particular, with respect, to sialylation.					
31907284	0	20	theme	Region	81:86	arg1	Glycosylation					57:69	the Functional Glycosylation	42:69	the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses	42:134	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	4	21	theme	glycans	807:813	arg1	types					789:793	the types	785:793	the types of N-linked glycans between matched pairs of Fc mutants	785:849	We show that the types of N-linked glycans between matched pairs of Fc mutants vary greatly and in particular, with respect, to sialylation.					
31907284	5	22	dep	receptors	1051:1059	arg1	either					1026:1031	either	1026:1031	either	1026:1031	These cell line effects on glycosylation profoundly influence the ability of the engineered Fcs to interact with either human or pathogen receptors.					
31907284	5	22	dep	receptors	1051:1059	arg1	pathogen					1042:1049	pathogen	1042:1049	pathogen	1042:1049	These cell line effects on glycosylation profoundly influence the ability of the engineered Fcs to interact with either human or pathogen receptors.					
31907284	2	23	theme	cell	363:366	arg1	line					368:371	The host cell line	354:371	The host cell line used to produce IgG	354:391	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	6	24	theme	293-F	1259:1263	arg1	cells					1265:1269	human endothelial kidney 293-F cells	1234:1269	human endothelial kidney 293-F cells	1234:1269	For example, we describe Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells.					
31907284	2	25	theme	profile	600:606	arg1	specificity					536:546	specificity	536:546	specificity	536:546	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	2	25	theme	profile	600:606	arg1	heterogeneity					552:564	heterogeneity	552:564	heterogeneity	552:564	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	2	26	dep	specificity	536:546	arg1	the					532:534	the	532:534	the	532:534	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	3	27	theme	human	704:708	arg1	293-F					729:733	the human endothelial kidney 293-F	700:733	the human endothelial kidney 293-F	700:733	In this study, we compare two panels of glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems.					
31907284	2	28	theme	host	358:361	arg1	line					368:371	The host cell line	354:371	The host cell line used to produce IgG	354:391	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	2	29	theme	major	401:405	arg1	role					407:410	a major role	399:410	a major role	399:410	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	1	30	theme	fine	242:245	arg1	structure					247:255	fine structure	242:255	fine structure	242:255	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	2	31	theme	glycosylation	586:598	arg1	profile					600:606	the final IgG-Fc glycosylation profile	569:606	the final IgG-Fc glycosylation profile	569:606	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	2	32	theme	final	573:577	arg1	profile					600:606	the final IgG-Fc glycosylation profile	569:606	the final IgG-Fc glycosylation profile	569:606	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	0	33	theme	Cell	15:18	arg1	Line					20:23	Host Cell Line	10:23	Host Cell Line	10:23	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	0	34	theme	IgG1	97:100	arg1	Inhibitors					102:111	Human IgG1 Inhibitors	91:111	Human IgG1 Inhibitors of Influenza B Viruses	91:134	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	0	35	theme	Host	10:13	arg1	Line					20:23	Host Cell Line	10:23	Host Cell Line	10:23	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	3	36	theme	endothelial	710:720	arg1	293-F					729:733	the human endothelial kidney 293-F	700:733	the human endothelial kidney 293-F	700:733	In this study, we compare two panels of glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems.					
31907284	0	37	theme	Human	91:95	arg1	Inhibitors					102:111	Human IgG1 Inhibitors	91:111	Human IgG1 Inhibitors of Influenza B Viruses	91:134	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	3	38	theme	kidney	722:727	arg1	293-F					729:733	the human endothelial kidney 293-F	700:733	the human endothelial kidney 293-F	700:733	In this study, we compare two panels of glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems.					
31907284	1	39	attach	attached	204:211	arg2	carbohydrate					191:202	a conserved N-linked carbohydrate	170:202	a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes	170:351	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	1	39	attach	attached	204:211	arg1	Fc					220:221	the Fc	216:221	the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes	216:351	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	1	40	theme	conserved	172:180	arg1	carbohydrate					191:202	a conserved N-linked carbohydrate	170:202	a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes	170:351	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	0	41	theme	Line	20:23	arg1	Choice					0:5	Choice	0:5	Choice of Host Cell Line	0:23	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	3	42	theme	ovary-K1	754:761	arg1	systems					763:769	Chinese hamster ovary-K1 systems	738:769	Chinese hamster ovary-K1 systems	738:769	In this study, we compare two panels of glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems.					
31907284	0	43	theme	Inhibitors	102:111	arg1	Region					81:86	the Fc Region	74:86	the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses	74:134	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	3	44	theme	Chinese	738:744	arg1	systems					763:769	Chinese hamster ovary-K1 systems	738:769	Chinese hamster ovary-K1 systems	738:769	In this study, we compare two panels of glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems.					
31907284	6	45	theme	hamster	1205:1211	arg1	ovary-K1					1213:1220	Chinese hamster ovary-K1	1197:1220	Chinese hamster ovary-K1	1197:1220	For example, we describe Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells.					
31907284	1	46	theme	N-linked	182:189	arg1	carbohydrate					191:202	a conserved N-linked carbohydrate	170:202	a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes	170:351	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	0	47	theme	B	126:126	arg1	Viruses					128:134	Influenza B Viruses	116:134	Influenza B Viruses	116:134	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	4	48	theme	N-linked	798:805	arg1	glycans					807:813	N-linked glycans	798:813	N-linked glycans between matched pairs of Fc mutants	798:849	We show that the types of N-linked glycans between matched pairs of Fc mutants vary greatly and in particular, with respect, to sialylation.					
31907284	4	49	theme	matched	823:829	arg1	pairs					831:835	matched pairs	823:835	matched pairs of Fc mutants	823:849	We show that the types of N-linked glycans between matched pairs of Fc mutants vary greatly and in particular, with respect, to sialylation.					
31907284	6	50	theme	Fc	1087:1088	arg1	mutants					1090:1096	Fc mutants	1087:1096	Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells	1087:1269	For example, we describe Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells.					
31907284	1	51	theme	in	285:286	arg1	immunogenicity					293:306	their in vivo immunogenicity	279:306	their in vivo immunogenicity	279:306	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	0	52	theme	Influenza	116:124	arg1	Viruses					128:134	Influenza B Viruses	116:134	Influenza B Viruses	116:134	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	5	53	theme	Fcs	1005:1007	arg1	ability					979:985	the ability	975:985	the ability of the engineered Fcs to interact with either human or pathogen receptors	975:1059	These cell line effects on glycosylation profoundly influence the ability of the engineered Fcs to interact with either human or pathogen receptors.					
31907284	5	54	theme	engineered	994:1003	arg1	Fcs					1005:1007	the engineered Fcs	990:1007	the engineered Fcs	990:1007	These cell line effects on glycosylation profoundly influence the ability of the engineered Fcs to interact with either human or pathogen receptors.					
31907284	2	55	gly	heterogeneity	552:564	arg1	profile					600:606	the final IgG-Fc glycosylation profile	569:606	the final IgG-Fc glycosylation profile	569:606	The host cell line used to produce IgG plays a major role in this glycosylation, as different systems express different glycosylation enzymes and transporters that contribute to the specificity and heterogeneity of the final IgG-Fc glycosylation profile.					
31907284	3	56	theme	hamster	746:752	arg1	systems					763:769	Chinese hamster ovary-K1 systems	738:769	Chinese hamster ovary-K1 systems	738:769	In this study, we compare two panels of glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems.					
31907284	6	57	theme	kidney	1252:1257	arg1	cells					1265:1269	human endothelial kidney 293-F cells	1234:1269	human endothelial kidney 293-F cells	1234:1269	For example, we describe Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells.					
31907284	0	58	theme	Viruses	128:134	arg1	Inhibitors					102:111	Human IgG1 Inhibitors	91:111	Human IgG1 Inhibitors of Influenza B Viruses	91:134	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	0	59	theme	Functional	46:55	arg1	Glycosylation					57:69	the Functional Glycosylation	42:69	the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses	42:134	Choice of Host Cell Line Is Essential for the Functional Glycosylation of the Fc Region of Human IgG1 Inhibitors of Influenza B Viruses.					
31907284	1	60	contain	carry	164:168	arg1	glycoproteins					145:157	glycoproteins	145:157	glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes	145:351	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	1	60	contain	carry	164:168	arg2	carbohydrate					191:202	a conserved N-linked carbohydrate	170:202	a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes	170:351	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	1	60	contain	carry	164:168	arg1	Abs					137:139	Abs	137:139	Abs	137:139	Abs are glycoproteins that carry a conserved N-linked carbohydrate attached to the Fc whose presence and fine structure profoundly impacts on their in vivo immunogenicity, pharmacokinetics, and functional attributes.					
31907284	6	61	theme	erythrocytes	1166:1177	arg1	agglutination					1143:1155	influenza B-mediated agglutination	1122:1155	influenza B-mediated agglutination of human erythrocytes	1122:1177	For example, we describe Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells.					
31907284	6	62	theme	B-mediated	1132:1141	arg1	agglutination					1143:1155	influenza B-mediated agglutination	1122:1155	influenza B-mediated agglutination of human erythrocytes	1122:1177	For example, we describe Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells.					
31907284	5	63	dep	either	1026:1031	arg1	human					1033:1037	human	1033:1037	human	1033:1037	These cell line effects on glycosylation profoundly influence the ability of the engineered Fcs to interact with either human or pathogen receptors.					
31907284	3	64	theme	mutants	672:678	arg1	panels					639:644	two panels	635:644	two panels of glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems	635:769	In this study, we compare two panels of glycan-adapted IgG1-Fc mutants expressed in either the human endothelial kidney 293-F or Chinese hamster ovary-K1 systems.					
31907284	6	65	theme	human	1160:1164	arg1	erythrocytes					1166:1177	human erythrocytes	1160:1177	human erythrocytes	1160:1177	For example, we describe Fc mutants that potently disrupted influenza B-mediated agglutination of human erythrocytes when expressed in Chinese hamster ovary-K1, but not in human endothelial kidney 293-F cells.					
31907284	5	66	from	effects	929:935	arg1	glycosylation					940:952	glycosylation	940:952	glycosylation	940:952	These cell line effects on glycosylation profoundly influence the ability of the engineered Fcs to interact with either human or pathogen receptors.					
34478702	7	0	theme	2-anthranilic	1066:1078	arg1	2AA					1086:1088	2AA	1086:1088	2AA	1086:1088	This method was compared to analysis of GSL glycans after cleavage by an Endoglycoceramidase (EGCase) enzyme and labeling with a fluorophore (2-anthranilic acid, 2AA).					
34478702	7	0	theme	2-anthranilic	1066:1078	arg1	acid					1080:1083	2-anthranilic acid	1066:1083	2-anthranilic acid	1066:1083	This method was compared to analysis of GSL glycans after cleavage by an Endoglycoceramidase (EGCase) enzyme and labeling with a fluorophore (2-anthranilic acid, 2AA).					
34478702	10	1	theme	cultured	1331:1338	arg1	cells					1346:1350	cultured human cells	1331:1350	cultured human cells (Jurkat T cells)	1331:1367	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	10	1	theme	cultured	1331:1338	arg1	cells					1362:1366	Jurkat T cells	1353:1366	Jurkat T cells	1353:1366	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	5	2	theme	amine-reactive	799:812	arg1	fluorophore					814:824	an amine-reactive fluorophore	796:824	an amine-reactive fluorophore	796:824	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	10	3	theme	porcine	1392:1398	arg1	homogenate					1380:1389	tissue homogenate	1373:1389	tissue homogenate (porcine brain)	1373:1405	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	10	3	theme	porcine	1392:1398	arg1	brain					1400:1404	porcine brain	1392:1404	porcine brain	1392:1404	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	6	4	theme	standard	914:921	arg1	addition					890:897	addition	890:897	addition of an external standard	890:921	The sample was then analyzed by HPLC-FLD-MS and quantitated by addition of an external standard.					
34478702	8	5	theme	lyso-GSL	1221:1228	arg1	analysis					1209:1216	analysis	1209:1216	analysis of lyso-GSL	1209:1228	The two methods are complementary, with EGCase providing improved signal (due to fewer species) and SCDase providing analysis of lyso-GSL.					
34478702	7	6	with	labeling	1037:1044	arg1	fluorophore					1053:1063	a fluorophore	1051:1063	a fluorophore (2-anthranilic acid, 2AA)	1051:1089	This method was compared to analysis of GSL glycans after cleavage by an Endoglycoceramidase (EGCase) enzyme and labeling with a fluorophore (2-anthranilic acid, 2AA).					
34478702	4	7	theme	Sph	659:661	arg1	base					663:666	the Sph base	655:666	the Sph base	655:666	Here, we develop a new strategy for the analysis of lyso-GSL (l-GSL), GSL that retain linkage of the glycan headgroup with the Sph base.					
34478702	1	8	theme	Lipid	71:75	arg1	components					77:86	Lipid components	71:86	Lipid components of cells and tissues	71:107	Lipid components of cells and tissues feature a large diversity of structures that present a challenging problem for molecular analysis.					
34478702	2	9	theme	glycolipid	290:299	arg1	component					301:309	their major glycolipid component	278:309	their major glycolipid component	278:309	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	2	10	from	cells	235:239	arg1	Glycolipids					208:218	Glycolipids	208:218	Glycolipids from mammalian cells	208:239	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	4	11	theme	glycan	633:638	arg1	headgroup					640:648	the glycan headgroup	629:648	the glycan headgroup	629:648	Here, we develop a new strategy for the analysis of lyso-GSL (l-GSL), GSL that retain linkage of the glycan headgroup with the Sph base.					
34478702	7	12	theme	Endoglycoceramidase	997:1015	arg1	enzyme					1026:1031	an Endoglycoceramidase (EGCase) enzyme	994:1031	an Endoglycoceramidase (EGCase) enzyme	994:1031	This method was compared to analysis of GSL glycans after cleavage by an Endoglycoceramidase (EGCase) enzyme and labeling with a fluorophore (2-anthranilic acid, 2AA).					
34478702	8	13	theme	improved	1149:1156	arg1	signal					1158:1163	improved signal	1149:1163	improved signal (due to fewer species)	1149:1186	The two methods are complementary, with EGCase providing improved signal (due to fewer species) and SCDase providing analysis of lyso-GSL.					
34478702	2	14	theme	major	284:288	arg1	component					301:309	their major glycolipid component	278:309	their major glycolipid component	278:309	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	1	15	theme	challenging	164:174	arg1	problem					176:182	a challenging problem	162:182	a challenging problem for molecular analysis	162:205	Lipid components of cells and tissues feature a large diversity of structures that present a challenging problem for molecular analysis.					
34478702	8	16	dep	signal	1158:1163	arg1	due					1166:1168	due	1166:1168	due	1166:1168	The two methods are complementary, with EGCase providing improved signal (due to fewer species) and SCDase providing analysis of lyso-GSL.					
34478702	2	17	dep	acid	416:419	arg1	tails					443:447	tails	443:447	tails	443:447	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	10	18	theme	T	1360:1360	arg1	cells					1346:1350	cultured human cells	1331:1350	cultured human cells (Jurkat T cells)	1331:1367	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	10	18	theme	T	1360:1360	arg1	cells					1362:1366	Jurkat T cells	1353:1366	Jurkat T cells	1353:1366	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	3	19	theme	low	500:502	arg1	abundance					504:512	the low abundance	496:512	the low abundance of these species	496:529	Analysis of intact GSLs is challenging due to the low abundance of these species.					
34478702	5	20	theme	sample	713:718	arg1	digestion					694:702	digestion	694:702	digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase)	694:766	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	1	21	theme	cells	91:95	arg1	components					77:86	Lipid components	71:86	Lipid components of cells and tissues	71:107	Lipid components of cells and tissues feature a large diversity of structures that present a challenging problem for molecular analysis.					
34478702	4	22	with	linkage	618:624	arg1	base					663:666	the Sph base	655:666	the Sph base	655:666	Here, we develop a new strategy for the analysis of lyso-GSL (l-GSL), GSL that retain linkage of the glycan headgroup with the Sph base.					
34478702	2	23	theme	sphingosine	425:435	arg1	structure					393:401	the structure	389:401	the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails	357:447	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	2	23	theme	sphingosine	425:435	arg1	headgroup					368:376	the glycan headgroup	357:376	the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails	357:447	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	10	24	from	method	1321:1326	arg1	homogenate					1380:1389	tissue homogenate	1373:1389	tissue homogenate (porcine brain)	1373:1405	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	10	24	from	method	1321:1326	arg1	brain					1400:1404	porcine brain	1392:1404	porcine brain	1392:1404	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	10	24	from	method	1321:1326	arg1	cells					1362:1366	Jurkat T cells	1353:1366	Jurkat T cells	1353:1366	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	10	24	from	method	1321:1326	arg1	cells					1346:1350	cultured human cells	1331:1350	cultured human cells (Jurkat T cells)	1331:1367	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	7	25	dep	fluorophore	1053:1063	arg1	2AA					1086:1088	2AA	1086:1088	2AA	1086:1088	This method was compared to analysis of GSL glycans after cleavage by an Endoglycoceramidase (EGCase) enzyme and labeling with a fluorophore (2-anthranilic acid, 2AA).					
34478702	7	25	dep	fluorophore	1053:1063	arg1	acid					1080:1083	2-anthranilic acid	1066:1083	2-anthranilic acid	1066:1083	This method was compared to analysis of GSL glycans after cleavage by an Endoglycoceramidase (EGCase) enzyme and labeling with a fluorophore (2-anthranilic acid, 2AA).					
34478702	10	26	theme	Jurkat	1353:1358	arg1	cells					1346:1350	cultured human cells	1331:1350	cultured human cells (Jurkat T cells)	1331:1367	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	10	26	theme	Jurkat	1353:1358	arg1	cells					1362:1366	Jurkat T cells	1353:1366	Jurkat T cells	1353:1366	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	1	27	theme	molecular	188:196	arg1	analysis					198:205	molecular analysis	188:205	molecular analysis	188:205	Lipid components of cells and tissues feature a large diversity of structures that present a challenging problem for molecular analysis.					
34478702	5	28	theme	sphingolipid	725:736	arg1	SCDase					760:765	SCDase	760:765	SCDase	760:765	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	5	28	theme	sphingolipid	725:736	arg1	N-deacylase					747:757	sphingolipid ceramide N-deacylase	725:757	sphingolipid ceramide N-deacylase (SCDase)	725:766	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	5	29	dep	begins	682:687	arg1	followed					769:776	followed	769:776	followed by labelling with an amine-reactive fluorophore	769:824	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	1	30	theme	tissues	101:107	arg1	components					77:86	Lipid components	71:86	Lipid components of cells and tissues	71:107	Lipid components of cells and tissues feature a large diversity of structures that present a challenging problem for molecular analysis.					
34478702	10	31	theme	human	1340:1344	arg1	cells					1346:1350	cultured human cells	1331:1350	cultured human cells (Jurkat T cells)	1331:1367	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	10	31	theme	human	1340:1344	arg1	cells					1362:1366	Jurkat T cells	1353:1366	Jurkat T cells	1353:1366	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	2	32	theme	fatty	410:414	arg1	acid					416:419	the fatty acid	406:419	the fatty acid	406:419	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	2	33	theme	acid	416:419	arg1	structure					393:401	the structure	389:401	the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails	357:447	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	2	33	theme	acid	416:419	arg1	headgroup					368:376	the glycan headgroup	357:376	the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails	357:447	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	5	34	with	digestion	694:702	arg1	SCDase					760:765	SCDase	760:765	SCDase	760:765	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	5	34	with	digestion	694:702	arg1	N-deacylase					747:757	sphingolipid ceramide N-deacylase	725:757	sphingolipid ceramide N-deacylase (SCDase)	725:766	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	3	35	theme	species	523:529	arg1	abundance					504:512	the low abundance	496:512	the low abundance of these species	496:529	Analysis of intact GSLs is challenging due to the low abundance of these species.					
34478702	1	36	theme	structures	138:147	arg1	diversity					125:133	a large diversity	117:133	a large diversity of structures that present a challenging problem for molecular analysis	117:205	Lipid components of cells and tissues feature a large diversity of structures that present a challenging problem for molecular analysis.					
34478702	3	37	theme	GSLs	469:472	arg1	Analysis					450:457	Analysis	450:457	Analysis of intact GSLs	450:472	Analysis of intact GSLs is challenging due to the low abundance of these species.					
34478702	7	38	theme	GSL	964:966	arg1	glycans					968:974	GSL glycans	964:974	GSL glycans	964:974	This method was compared to analysis of GSL glycans after cleavage by an Endoglycoceramidase (EGCase) enzyme and labeling with a fluorophore (2-anthranilic acid, 2AA).					
34478702	0	39	theme	SCDase	37:42	arg1	digestion					44:52	SCDase digestion	37:52	SCDase digestion	37:52	Profiling of glycosphingolipids with SCDase digestion and HPLC-FLD-MS.					
34478702	7	40	theme	glycans	968:974	arg1	analysis					952:959	analysis	952:959	analysis of GSL glycans	952:974	This method was compared to analysis of GSL glycans after cleavage by an Endoglycoceramidase (EGCase) enzyme and labeling with a fluorophore (2-anthranilic acid, 2AA).					
34478702	9	41	theme	species	1293:1299	arg1	composition					1274:1284	Sph composition	1270:1284	Sph composition of GSL species	1270:1299	Importantly the SCDase method provides Sph composition of GSL species.					
34478702	2	42	theme	structure	393:401	arg1	identity					345:352	the identity	341:352	the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails	341:447	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	8	43	theme	fewer	1173:1177	arg1	species					1179:1185	fewer species	1173:1185	fewer species	1173:1185	The two methods are complementary, with EGCase providing improved signal (due to fewer species) and SCDase providing analysis of lyso-GSL.					
34478702	9	44	theme	SCDase	1247:1252	arg1	method					1254:1259	the SCDase method	1243:1259	the SCDase method	1243:1259	Importantly the SCDase method provides Sph composition of GSL species.					
34478702	7	45	with	cleavage	982:989	arg1	fluorophore					1053:1063	a fluorophore	1051:1063	a fluorophore (2-anthranilic acid, 2AA)	1051:1089	This method was compared to analysis of GSL glycans after cleavage by an Endoglycoceramidase (EGCase) enzyme and labeling with a fluorophore (2-anthranilic acid, 2AA).					
34478702	3	46	theme	intact	462:467	arg1	GSLs					469:472	intact GSLs	462:472	intact GSLs	462:472	Analysis of intact GSLs is challenging due to the low abundance of these species.					
34478702	5	47	theme	GSL	709:711	arg1	sample					713:718	a GSL sample	707:718	a GSL sample	707:718	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	5	48	with	labelling	781:789	arg1	fluorophore					814:824	an amine-reactive fluorophore	796:824	an amine-reactive fluorophore	796:824	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	2	49	theme	mammalian	225:233	arg1	cells					235:239	mammalian cells	225:239	mammalian cells	225:239	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	1	50	theme	large	119:123	arg1	diversity					125:133	a large diversity	117:133	a large diversity of structures that present a challenging problem for molecular analysis	117:205	Lipid components of cells and tissues feature a large diversity of structures that present a challenging problem for molecular analysis.					
34478702	0	51	with	Profiling	0:8	arg1	digestion					44:52	SCDase digestion	37:52	SCDase digestion	37:52	Profiling of glycosphingolipids with SCDase digestion and HPLC-FLD-MS.					
34478702	0	51	with	Profiling	0:8	arg1	HPLC-FLD-MS					58:68	HPLC-FLD-MS	58:68	HPLC-FLD-MS	58:68	Profiling of glycosphingolipids with SCDase digestion and HPLC-FLD-MS.					
34478702	9	52	theme	Sph	1270:1272	arg1	composition					1274:1284	Sph composition	1270:1284	Sph composition of GSL species	1270:1299	Importantly the SCDase method provides Sph composition of GSL species.					
34478702	10	53	theme	tissue	1373:1378	arg1	homogenate					1380:1389	tissue homogenate	1373:1389	tissue homogenate (porcine brain)	1373:1405	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	10	53	theme	tissue	1373:1378	arg1	brain					1400:1404	porcine brain	1392:1404	porcine brain	1392:1404	We demonstrate the method on cultured human cells (Jurkat T cells) and tissue homogenate (porcine brain).					
34478702	5	54	theme	ceramide	738:745	arg1	SCDase					760:765	SCDase	760:765	SCDase	760:765	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	5	54	theme	ceramide	738:745	arg1	N-deacylase					747:757	sphingolipid ceramide N-deacylase	725:757	sphingolipid ceramide N-deacylase (SCDase)	725:766	The analysis begins with digestion of a GSL sample with sphingolipid ceramide N-deacylase (SCDase), followed by labelling with an amine-reactive fluorophore.					
34478702	4	55	theme	headgroup	640:648	arg1	linkage					618:624	linkage	618:624	linkage of the glycan headgroup with the Sph base	618:666	Here, we develop a new strategy for the analysis of lyso-GSL (l-GSL), GSL that retain linkage of the glycan headgroup with the Sph base.					
34478702	4	56	theme	new	551:553	arg1	strategy					555:562	a new strategy	549:562	a new strategy for the analysis of lyso-GSL (l-GSL), GSL that retain linkage of the glycan headgroup with the Sph base	549:666	Here, we develop a new strategy for the analysis of lyso-GSL (l-GSL), GSL that retain linkage of the glycan headgroup with the Sph base.					
34478702	4	57	theme	lyso-GSL	584:591	arg1	analysis					572:579	the analysis	568:579	the analysis of lyso-GSL (l-GSL), GSL that retain linkage of the glycan headgroup with the Sph base	568:666	Here, we develop a new strategy for the analysis of lyso-GSL (l-GSL), GSL that retain linkage of the glycan headgroup with the Sph base.					
34478702	2	58	theme	headgroup	368:376	arg1	identity					345:352	the identity	341:352	the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails	341:447	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	2	59	contain	contain	241:247	arg2	glycosphingolipids					249:266	glycosphingolipids	249:266	glycosphingolipids (GSLs)	249:273	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	2	59	contain	contain	241:247	arg2	GSLs					269:272	GSLs	269:272	GSLs	269:272	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	2	59	contain	contain	241:247	arg1	Glycolipids					208:218	Glycolipids	208:218	Glycolipids from mammalian cells	208:239	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	0	60	theme	glycosphingolipids	13:30	arg1	Profiling					0:8	Profiling	0:8	Profiling of glycosphingolipids with SCDase digestion and HPLC-FLD-MS.	0:69	Profiling of glycosphingolipids with SCDase digestion and HPLC-FLD-MS.					
34478702	2	61	theme	glycan	361:366	arg1	headgroup					368:376	the glycan headgroup	357:376	the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails	357:447	Glycolipids from mammalian cells contain glycosphingolipids (GSLs) as their major glycolipid component, and these structures vary in the identity of the glycan headgroup as well as the structure of the fatty acid and sphingosine (Sph) tails.					
34478702	9	62	theme	GSL	1289:1291	arg1	species					1293:1299	GSL species	1289:1299	GSL species	1289:1299	Importantly the SCDase method provides Sph composition of GSL species.					
34761861	6	0	theme	representative	1099:1112	arg1	serum					1117:1121	a representative MS serum	1097:1121	a representative MS serum	1097:1121	Specific antibodies from a representative MS serum, were successfully depleted using a Sepharose resin bearing the new glucosylated multivalent conjugate, as confirmed by ELISA.					
34761861	3	1	theme	40 kDa	673:678	arg1	scaffold					688:695	a 40 kDa dextran scaffold	671:695	a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC)	671:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	2	2	theme	multiple	375:382	arg1	sclerosis					384:392	multiple sclerosis	375:392	multiple sclerosis (MS) patient sera	375:410	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	2	2	theme	multiple	375:382	arg1	MS					395:396	MS	395:396	MS	395:396	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	6	3	theme	glucosylated	1191:1202	arg1	conjugate					1216:1224	the new glucosylated multivalent conjugate	1183:1224	the new glucosylated multivalent conjugate	1183:1224	Specific antibodies from a representative MS serum, were successfully depleted using a Sepharose resin bearing the new glucosylated multivalent conjugate, as confirmed by ELISA.					
34761861	3	4	theme	C-terminal	541:550	arg1	epitope					578:584	the C-terminal HMW1ct(1347-1354) minimal epitope	537:584	the C-terminal HMW1ct(1347-1354) minimal epitope	537:584	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	7	5	theme	affinity	1433:1440	arg1	antibodies					1442:1451	specific high affinity antibodies	1419:1451	specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc)	1419:1500	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	7	6	theme	high	1337:1340	arg1	autoantibodies					1351:1364	high affinity autoantibodies	1337:1364	high affinity autoantibodies	1337:1364	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	3	7	theme	minimal	570:576	arg1	epitope					578:584	the C-terminal HMW1ct(1347-1354) minimal epitope	537:584	the C-terminal HMW1ct(1347-1354) minimal epitope	537:584	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	1	8	theme	interesting	189:199	arg1	tools					201:205	interesting tools	189:205	interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity	189:330	Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity.					
34761861	7	9	theme	glcosylated	1473:1483	arg1	Glc					1497:1499	Glc	1497:1499	Glc	1497:1499	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	7	9	theme	glcosylated	1473:1483	arg1	Asn					1493:1495	a minimally glcosylated epitope Asn	1461:1495	a minimally glcosylated epitope Asn(Glc)	1461:1500	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	4	10	theme	peptide	875:881	arg1	loading					883:889	the peptide loading	871:889	the peptide loading (19.5 %)	871:898	Quantitative NMR measurements allowed the characterization of the peptide loading (19.5 %) on the multivalent dextran conjugate.					
34761861	4	10	theme	peptide	875:881	arg1	%					897:897	19.5 %	892:897	19.5 %	892:897	Quantitative NMR measurements allowed the characterization of the peptide loading (19.5 %) on the multivalent dextran conjugate.					
34761861	7	11	theme	specific	1419:1426	arg1	antibodies					1442:1451	specific high affinity antibodies	1419:1451	specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc)	1419:1500	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	3	12	theme	analogue	618:625	arg1	-NH2					657:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2	599:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC)	599:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	3	13	theme	HMW1ct	552:557	arg1	epitope					578:584	the C-terminal HMW1ct(1347-1354) minimal epitope	537:584	the C-terminal HMW1ct(1347-1354) minimal epitope	537:584	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	3	14	theme	alkyne-azide	769:780	arg1	CuAAC					801:805	CuAAC	801:805	CuAAC	801:805	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	3	14	theme	alkyne-azide	769:780	arg1	reaction					791:798	alkyne-azide coupling reaction	769:798	alkyne-azide coupling reaction (CuAAC)	769:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	5	15	theme	antibodies	1049:1058	arg1	properties					997:1006	optimal capturing properties	979:1006	optimal capturing properties of both IgG and, more interestingly, IgM antibodies	979:1058	This novel polymeric structure displayed optimal capturing properties of both IgG and, more interestingly, IgM antibodies in MS sera.					
34761861	4	16	theme	multivalent	907:917	arg1	conjugate					927:935	the multivalent dextran conjugate	903:935	the multivalent dextran conjugate	903:935	Quantitative NMR measurements allowed the characterization of the peptide loading (19.5 %) on the multivalent dextran conjugate.					
34761861	7	17	theme	autoimmune	1379:1388	arg1	patients					1390:1397	autoimmune patients	1379:1397	autoimmune patients	1379:1397	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	5	18	theme	MS	1063:1064	arg1	sera					1066:1069	MS sera	1063:1069	MS sera	1063:1069	This novel polymeric structure displayed optimal capturing properties of both IgG and, more interestingly, IgM antibodies in MS sera.					
34761861	1	19	theme	peptide	165:171	arg1	antigens					173:180	peptide antigens	165:180	peptide antigens	165:180	Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity.					
34761861	0	20	theme	Multivalent	78:88	arg1	Conjugate					98:106	a Multivalent Dextran Conjugate	76:106	a Multivalent Dextran Conjugate	76:106	Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate.					
34761861	5	21	theme	capturing	987:995	arg1	properties					997:1006	optimal capturing properties	979:1006	optimal capturing properties of both IgG and, more interestingly, IgM antibodies	979:1058	This novel polymeric structure displayed optimal capturing properties of both IgG and, more interestingly, IgM antibodies in MS sera.					
34761861	7	22	from	sera	1371:1374	arg1	purification					1321:1332	the selective purification	1307:1332	the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients	1307:1546	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	2	23	theme	Haemophilus	494:504	arg1	NTHi					518:521	NTHi	518:521	NTHi	518:521	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	2	23	theme	Haemophilus	494:504	arg1	influenzae					506:515	non-typeable Haemophilus influenzae	481:515	non-typeable Haemophilus influenzae (NTHi)	481:522	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	2	24	theme	patient	399:405	arg1	sera					407:410	multiple sclerosis (MS) patient sera	375:410	multiple sclerosis (MS) patient sera	375:410	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	0	25	theme	Selective	0:8	arg1	Capture					10:16	Selective Capture	0:16	Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate	0:106	Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate.					
34761861	1	26	theme	cooperative	291:301	arg1	effects					303:309	cooperative effects	291:309	cooperative effects	291:309	Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity.					
34761861	5	27	theme	polymeric	949:957	arg1	structure					959:967	This novel polymeric structure	938:967	This novel polymeric structure	938:967	This novel polymeric structure displayed optimal capturing properties of both IgG and, more interestingly, IgM antibodies in MS sera.					
34761861	7	28	theme	affinity	1342:1349	arg1	autoantibodies					1351:1364	high affinity autoantibodies	1337:1364	high affinity autoantibodies	1337:1364	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	2	29	theme	protein	463:469	arg1	HMW1ct					471:476	hyperglucosylated adhesin protein HMW1ct	437:476	hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi)	437:522	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	2	30	theme	hyperglucosylated	437:453	arg1	HMW1ct					471:476	hyperglucosylated adhesin protein HMW1ct	437:476	hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi)	437:522	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	0	31	theme	NTHi	41:44	arg1	Antibodies					62:71	Anti-N-glucosylated NTHi Adhesin Peptide Antibodies	21:71	Anti-N-glucosylated NTHi Adhesin Peptide Antibodies	21:71	Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate.					
34761861	7	32	theme	selective	1311:1319	arg1	purification					1321:1332	the selective purification	1307:1332	the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients	1307:1546	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	0	33	theme	Peptide	54:60	arg1	Antibodies					62:71	Anti-N-glucosylated NTHi Adhesin Peptide Antibodies	21:71	Anti-N-glucosylated NTHi Adhesin Peptide Antibodies	21:71	Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate.					
34761861	7	34	theme	multiple	1515:1522	arg1	MS					1535:1536	MS	1535:1536	MS	1535:1536	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	7	34	theme	multiple	1515:1522	arg1	sclerosis					1524:1532	multiple sclerosis	1515:1532	multiple sclerosis (MS) patients	1515:1546	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	5	35	theme	IgM	1045:1047	arg1	antibodies					1049:1058	IgM antibodies	1045:1058	IgM antibodies	1045:1058	This novel polymeric structure displayed optimal capturing properties of both IgG and, more interestingly, IgM antibodies in MS sera.					
34761861	7	36	from	purification	1321:1332	arg1	general					1403:1409	general	1403:1409	general	1403:1409	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	5	37	theme	IgG	1016:1018	arg1	properties					997:1006	optimal capturing properties	979:1006	optimal capturing properties of both IgG and, more interestingly, IgM antibodies	979:1058	This novel polymeric structure displayed optimal capturing properties of both IgG and, more interestingly, IgM antibodies in MS sera.					
34761861	7	38	theme	promising	1276:1284	arg1	proof-of-concept					1286:1301	a promising proof-of-concept	1274:1301	a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients	1274:1546	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	4	39	theme	Quantitative	809:820	arg1	measurements					826:837	Quantitative NMR measurements	809:837	Quantitative NMR measurements	809:837	Quantitative NMR measurements allowed the characterization of the peptide loading (19.5 %) on the multivalent dextran conjugate.					
34761861	6	40	theme	MS	1114:1115	arg1	serum					1117:1121	a representative MS serum	1097:1121	a representative MS serum	1097:1121	Specific antibodies from a representative MS serum, were successfully depleted using a Sepharose resin bearing the new glucosylated multivalent conjugate, as confirmed by ELISA.					
34761861	2	41	theme	sclerosis	384:392	arg1	sera					407:410	multiple sclerosis (MS) patient sera	375:410	multiple sclerosis (MS) patient sera	375:410	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	1	42	theme	patient	271:277	arg1	sera					279:282	patient sera	271:282	patient sera	271:282	Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity.					
34761861	4	43	theme	loading	883:889	arg1	characterization					851:866	the characterization	847:866	the characterization of the peptide loading (19.5 %) on the multivalent dextran conjugate	847:935	Quantitative NMR measurements allowed the characterization of the peptide loading (19.5 %) on the multivalent dextran conjugate.					
34761861	3	44	theme	dextran	680:686	arg1	scaffold					688:695	a 40 kDa dextran scaffold	671:695	a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC)	671:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	7	45	theme	epitope	1485:1491	arg1	Glc					1497:1499	Glc	1497:1499	Glc	1497:1499	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	7	45	theme	epitope	1485:1491	arg1	Asn					1493:1495	a minimally glcosylated epitope Asn	1461:1495	a minimally glcosylated epitope Asn(Glc)	1461:1500	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	6	46	theme	new	1187:1189	arg1	conjugate					1216:1224	the new glucosylated multivalent conjugate	1183:1224	the new glucosylated multivalent conjugate	1183:1224	Specific antibodies from a representative MS serum, were successfully depleted using a Sepharose resin bearing the new glucosylated multivalent conjugate, as confirmed by ELISA.					
34761861	7	47	theme	antibodies	1442:1451	arg1	purification					1321:1332	the selective purification	1307:1332	the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients	1307:1546	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	3	48	theme	glycidyl-propargyl	711:728	arg1	moieties					730:737	glycidyl-propargyl moieties	711:737	glycidyl-propargyl moieties	711:737	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	3	49	theme	diglucosylated	603:616	arg1	-NH2					657:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2	599:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC)	599:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	6	50	theme	Sepharose	1159:1167	arg1	resin					1169:1173	a Sepharose resin	1157:1173	a Sepharose resin bearing the new glucosylated multivalent conjugate	1157:1224	Specific antibodies from a representative MS serum, were successfully depleted using a Sepharose resin bearing the new glucosylated multivalent conjugate, as confirmed by ELISA.					
34761861	7	51	theme	high	1428:1431	arg1	antibodies					1442:1451	specific high affinity antibodies	1419:1451	specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc)	1419:1500	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	4	52	theme	dextran	919:925	arg1	conjugate					927:935	the multivalent dextran conjugate	903:935	the multivalent dextran conjugate	903:935	Quantitative NMR measurements allowed the characterization of the peptide loading (19.5 %) on the multivalent dextran conjugate.					
34761861	3	53	theme	Glc	634:636	arg1	-NH2					657:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2	599:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC)	599:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	1	54	from	antibodies	257:266	arg1	sera					279:282	patient sera	271:282	patient sera	271:282	Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity.					
34761861	3	55	theme	Ac-KAN	627:632	arg1	-NH2					657:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2	599:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC)	599:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	3	56	theme	coupling	782:789	arg1	CuAAC					801:805	CuAAC	801:805	CuAAC	801:805	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	3	56	theme	coupling	782:789	arg1	reaction					791:798	alkyne-azide coupling reaction	769:798	alkyne-azide coupling reaction (CuAAC)	769:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	3	57	theme	Glc	643:645	arg1	-NH2					657:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2	599:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC)	599:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	1	58	theme	several	147:153	arg1	copies					155:160	several copies	147:160	several copies of peptide antigens	147:180	Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity.					
34761861	7	59	theme	patients	1390:1397	arg1	sera					1371:1374	sera	1371:1374	sera of autoimmune patients	1371:1397	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	3	60	theme	VTLN	638:641	arg1	-NH2					657:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2	599:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC)	599:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	6	61	theme	multivalent	1204:1214	arg1	conjugate					1216:1224	the new glucosylated multivalent conjugate	1183:1224	the new glucosylated multivalent conjugate	1183:1224	Specific antibodies from a representative MS serum, were successfully depleted using a Sepharose resin bearing the new glucosylated multivalent conjugate, as confirmed by ELISA.					
34761861	0	62	theme	Dextran	90:96	arg1	Conjugate					98:106	a Multivalent Dextran Conjugate	76:106	a Multivalent Dextran Conjugate	76:106	Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate.					
34761861	1	63	theme	antigens	173:180	arg1	copies					155:160	several copies	147:160	several copies of peptide antigens	147:180	Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity.					
34761861	5	64	theme	optimal	979:985	arg1	properties					997:1006	optimal capturing properties	979:1006	optimal capturing properties of both IgG and, more interestingly, IgM antibodies	979:1058	This novel polymeric structure displayed optimal capturing properties of both IgG and, more interestingly, IgM antibodies in MS sera.					
34761861	7	65	theme	autoantibodies	1351:1364	arg1	purification					1321:1332	the selective purification	1307:1332	the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients	1307:1546	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	4	66	from	characterization	851:866	arg1	conjugate					927:935	the multivalent dextran conjugate	903:935	the multivalent dextran conjugate	903:935	Quantitative NMR measurements allowed the characterization of the peptide loading (19.5 %) on the multivalent dextran conjugate.					
34761861	5	67	theme	novel	943:947	arg1	structure					959:967	This novel polymeric structure	938:967	This novel polymeric structure	938:967	This novel polymeric structure displayed optimal capturing properties of both IgG and, more interestingly, IgM antibodies in MS sera.					
34761861	6	68	theme	Specific	1072:1079	arg1	antibodies					1081:1090	Specific antibodies	1072:1090	Specific antibodies	1072:1090	Specific antibodies from a representative MS serum, were successfully depleted using a Sepharose resin bearing the new glucosylated multivalent conjugate, as confirmed by ELISA.					
34761861	2	69	theme	non-typeable	481:492	arg1	NTHi					518:521	NTHi	518:521	NTHi	518:521	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	2	69	theme	non-typeable	481:492	arg1	influenzae					506:515	non-typeable Haemophilus influenzae	481:515	non-typeable Haemophilus influenzae (NTHi)	481:522	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	0	70	theme	Anti-N-glucosylated	21:39	arg1	Antibodies					62:71	Anti-N-glucosylated NTHi Adhesin Peptide Antibodies	21:71	Anti-N-glucosylated NTHi Adhesin Peptide Antibodies	21:71	Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate.					
34761861	1	71	theme	Tentacle-like	109:121	arg1	polymers					123:130	Tentacle-like polymers	109:130	Tentacle-like polymers decorated with several copies of peptide antigens	109:180	Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity.					
34761861	3	72	theme	N3	653:654	arg1	-NH2					657:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2	599:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC)	599:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	2	73	theme	adhesin	455:461	arg1	HMW1ct					471:476	hyperglucosylated adhesin protein HMW1ct	437:476	hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi)	437:522	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	0	74	theme	Adhesin	46:52	arg1	Antibodies					62:71	Anti-N-glucosylated NTHi Adhesin Peptide Antibodies	21:71	Anti-N-glucosylated NTHi Adhesin Peptide Antibodies	21:71	Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate.					
34761861	1	75	theme	stronger	315:322	arg1	avidity					324:330	stronger avidity	315:330	stronger avidity	315:330	Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity.					
34761861	7	76	theme	patients	1539:1546	arg1	sera					1507:1510	sera	1507:1510	sera of multiple sclerosis (MS) patients	1507:1546	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	3	77	theme	TTG-K	647:651	arg1	-NH2					657:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2	599:660	the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC)	599:806	We selected the C-terminal HMW1ct(1347-1354) minimal epitope and prepared the diglucosylated analogue Ac-KAN(Glc)VTLN(Glc)TTG-K(N3 )-NH2 to graft a 40 kDa dextran scaffold modified with glycidyl-propargyl moieties to perform a copper catalyzed alkyne-azide coupling reaction (CuAAC).					
34761861	4	78	theme	NMR	822:824	arg1	measurements					826:837	Quantitative NMR measurements	809:837	Quantitative NMR measurements	809:837	Quantitative NMR measurements allowed the characterization of the peptide loading (19.5 %) on the multivalent dextran conjugate.					
34761861	0	79	theme	Antibodies	62:71	arg1	Capture					10:16	Selective Capture	0:16	Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate	0:106	Selective Capture of Anti-N-glucosylated NTHi Adhesin Peptide Antibodies by a Multivalent Dextran Conjugate.					
34761861	2	80	theme	influenzae	506:515	arg1	HMW1ct					471:476	hyperglucosylated adhesin protein HMW1ct	437:476	hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi)	437:522	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	7	81	theme	sclerosis	1524:1532	arg1	patients					1539:1546	multiple sclerosis (MS) patients	1515:1546	multiple sclerosis (MS) patients	1515:1546	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	2	82	from	sera	407:410	arg1	antibodies					359:368	antibodies	359:368	antibodies from multiple sclerosis (MS) patient sera	359:410	We previously showed that antibodies from multiple sclerosis (MS) patient sera preferentially recognize hyperglucosylated adhesin protein HMW1ct of non-typeable Haemophilus influenzae (NTHi).					
34761861	1	83	theme	circulating	245:255	arg1	antibodies					257:266	circulating antibodies	245:266	circulating antibodies in patient sera	245:282	Tentacle-like polymers decorated with several copies of peptide antigens can be interesting tools for increasing the ability to capture circulating antibodies in patient sera, using cooperative effects for stronger avidity.					
34761861	7	84	from	sera	1507:1510	arg1	sera					1371:1374	sera	1371:1374	sera of autoimmune patients	1371:1397	These results may offer a promising proof-of-concept for the selective purification of high affinity autoantibodies from sera of autoimmune patients, in general, and of specific high affinity antibodies against a minimally glcosylated epitope Asn(Glc) from sera of multiple sclerosis (MS) patients, in particular.					
34761861	6	85	from	serum	1117:1121	arg1	antibodies					1081:1090	Specific antibodies	1072:1090	Specific antibodies	1072:1090	Specific antibodies from a representative MS serum, were successfully depleted using a Sepharose resin bearing the new glucosylated multivalent conjugate, as confirmed by ELISA.					
32454127	7	0	theme	heparan	1244:1250	arg1	HS					1261:1262	HS	1261:1262	HS	1261:1262	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	7	0	theme	heparan	1244:1250	arg1	sulfate					1252:1258	heparan sulfate	1244:1258	heparan sulfate (HS)	1244:1263	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	3	1	from	glycans	464:470	arg1	PrP					509:511	PrP	509:511	PrP	509:511	In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.					
32454127	3	1	from	glycans	464:470	arg1	protein					500:506	the prion protein	490:506	the prion protein (PrP)	490:512	In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.					
32454127	9	2	theme	human	1706:1710	arg1	disease					1729:1735	human and animal prion disease	1706:1735	human and animal prion disease	1706:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	9	3	from	deposits	1631:1638	arg1	brain					1647:1651	the brain	1643:1651	the brain	1643:1651	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	9	4	theme	high	1578:1581	arg1	frequency					1583:1591	the high frequency	1574:1591	the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease	1574:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	0	5	theme	aggregate	90:98	arg1	size					100:103	aggregate size	90:103	aggregate size in prion disease	90:120	Prion protein post-translational modifications modulate heparan sulfate binding and limit aggregate size in prion disease.					
32454127	1	6	attach	linked	155:160	arg1	disease					183:189	neurodegenerative disease	165:189	neurodegenerative disease	165:189	Many aggregation-prone proteins linked to neurodegenerative disease are post-translationally modified during their biogenesis.					
32454127	1	6	attach	linked	155:160	arg2	proteins					146:153	Many aggregation-prone proteins	123:153	Many aggregation-prone proteins linked to neurodegenerative disease	123:189	Many aggregation-prone proteins linked to neurodegenerative disease are post-translationally modified during their biogenesis.					
32454127	5	7	theme	additional	704:713	arg1	PrP					744:746	tri-glycosylated PrP	727:746	tri-glycosylated PrP	727:746	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	5	7	theme	additional	704:713	arg1	glycan					719:724	an additional PrP glycan	701:724	an additional PrP glycan (tri-glycosylated PrP)	701:747	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	2	8	theme	disease	414:420	arg1	phenotype					422:430	the disease phenotype	410:430	the disease phenotype	410:430	In vivo pathogenesis studies have suggested that the presence of post-translational modifications can shift the aggregate assembly pathway and profoundly alter the disease phenotype.					
32454127	0	9	theme	prion	108:112	arg1	disease					114:120	prion disease	108:120	prion disease	108:120	Prion protein post-translational modifications modulate heparan sulfate binding and limit aggregate size in prion disease.					
32454127	3	10	from	GPI-anchor	476:485	arg1	PrP					509:511	PrP	509:511	PrP	509:511	In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.					
32454127	3	10	from	GPI-anchor	476:485	arg1	protein					500:506	the prion protein	490:506	the prion protein (PrP)	490:512	In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.					
32454127	5	11	theme	PrP	715:717	arg1	PrP					744:746	tri-glycosylated PrP	727:746	tri-glycosylated PrP	727:746	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	5	11	theme	PrP	715:717	arg1	glycan					719:724	an additional PrP glycan	701:724	an additional PrP glycan (tri-glycosylated PrP)	701:747	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	7	12	theme	other	1276:1280	arg1	prions					1309:1314	other predominantly GPI-anchored prions	1276:1314	other predominantly GPI-anchored prions	1276:1314	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	8	13	theme	co-factors	1457:1466	arg1	involvement					1439:1449	low involvement	1435:1449	low involvement of HS co-factors	1435:1466	Collectively, these results suggest that highly glycosylated PrP primarily converts as a GPI-anchored glycoform, with low involvement of HS co-factors, limiting PrP assembly mainly to oligomers.					
32454127	5	14	theme	neuronal	785:792	arg1	membranes					799:807	neuronal cell membranes	785:807	neuronal cell membranes	785:807	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	9	15	theme	prion	1723:1727	arg1	disease					1729:1735	human and animal prion disease	1706:1735	human and animal prion disease	1706:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	7	16	theme	low	1230:1232	arg1	levels					1234:1239	low levels	1230:1239	low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions	1230:1314	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	6	17	theme	glycophosphatidylinositol	1069:1093	arg1	anchor					1101:1106	the glycophosphatidylinositol (GPI) anchor	1065:1106	the glycophosphatidylinositol (GPI) anchor	1065:1106	These plaque-like deposits were largely non-congophilic and composed of full length, uncleaved PrP, indicating retention of the glycophosphatidylinositol (GPI) anchor.					
32454127	5	18	theme	cell	794:797	arg1	membranes					799:807	neuronal cell membranes	785:807	neuronal cell membranes	785:807	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	6	19	theme	plaque-like	947:957	arg1	deposits					959:966	These plaque-like deposits	941:966	These plaque-like deposits	941:966	These plaque-like deposits were largely non-congophilic and composed of full length, uncleaved PrP, indicating retention of the glycophosphatidylinositol (GPI) anchor.					
32454127	5	20	theme	prion-infected	662:675	arg1	mice					685:688	prion-infected knockin mice	662:688	prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP)	662:747	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	8	21	theme	glycosylated	1365:1376	arg1	PrP					1378:1380	highly glycosylated PrP	1358:1380	highly glycosylated PrP	1358:1380	Collectively, these results suggest that highly glycosylated PrP primarily converts as a GPI-anchored glycoform, with low involvement of HS co-factors, limiting PrP assembly mainly to oligomers.					
32454127	2	22	theme	modifications	334:346	arg1	presence					303:310	the presence	299:310	the presence of post-translational modifications	299:346	In vivo pathogenesis studies have suggested that the presence of post-translational modifications can shift the aggregate assembly pathway and profoundly alter the disease phenotype.					
32454127	3	23	theme	prion	494:498	arg1	PrP					509:511	PrP	509:511	PrP	509:511	In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.					
32454127	3	23	theme	prion	494:498	arg1	protein					500:506	the prion protein	490:506	the prion protein (PrP)	490:512	In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.					
32454127	9	24	located	observed	1694:1701	arg2	conversion					1674:1683	the rapid conversion	1664:1683	the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease	1574:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	9	24	located	observed	1694:1701	arg1	disease					1729:1735	human and animal prion disease	1706:1735	human and animal prion disease	1706:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	9	25	from	frequency	1583:1591	arg1	brain					1647:1651	the brain	1643:1651	the brain	1643:1651	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	5	26	theme	knockin	677:683	arg1	mice					685:688	prion-infected knockin mice	662:688	prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP)	662:747	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	7	27	theme	density	1144:1150	arg1	fractions					1152:1160	low density fractions	1140:1160	low density fractions following ultracentrifugation	1140:1190	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	2	28	theme	post-translational	315:332	arg1	modifications					334:346	post-translational modifications	315:346	post-translational modifications	315:346	In vivo pathogenesis studies have suggested that the presence of post-translational modifications can shift the aggregate assembly pathway and profoundly alter the disease phenotype.					
32454127	9	29	from	conversion	1674:1683	arg1	brain					1647:1651	the brain	1643:1651	the brain	1643:1651	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	0	30	theme	protein	6:12	arg1	modifications					33:45	Prion protein post-translational modifications	0:45	Prion protein post-translational modifications	0:45	Prion protein post-translational modifications modulate heparan sulfate binding and limit aggregate size in prion disease.					
32454127	5	31	theme	subarachnoid	820:831	arg1	space					833:837	the subarachnoid space	816:837	the subarachnoid space	816:837	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	9	32	theme	deposits	1631:1638	arg1	frequency					1583:1591	the high frequency	1574:1591	the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease	1574:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	9	32	theme	deposits	1631:1638	arg1	conversion					1674:1683	the rapid conversion	1664:1683	the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease	1574:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	7	33	theme	similar	1265:1271	arg1	levels					1234:1239	low levels	1230:1239	low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions	1230:1314	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	7	34	theme	sulfate	1252:1258	arg1	levels					1234:1239	low levels	1230:1239	low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions	1230:1314	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	5	35	theme	interstitial	921:932	arg1	fluid					934:938	the interstitial fluid	917:938	the interstitial fluid	917:938	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	0	36	theme	Prion	0:4	arg1	modifications					33:45	Prion protein post-translational modifications	0:45	Prion protein post-translational modifications	0:45	Prion protein post-translational modifications modulate heparan sulfate binding and limit aggregate size in prion disease.					
32454127	2	37	theme	assembly	372:379	arg1	pathway					381:387	the aggregate assembly pathway	358:387	the aggregate assembly pathway	358:387	In vivo pathogenesis studies have suggested that the presence of post-translational modifications can shift the aggregate assembly pathway and profoundly alter the disease phenotype.					
32454127	3	38	theme	prion	436:440	arg1	disease					442:448	prion disease	436:448	prion disease	436:448	In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.					
32454127	4	39	theme	aggregate	583:591	arg1	structure					593:601	aggregate structure	583:601	aggregate structure	583:601	However, the relevance of the two glycans to aggregate structure and disease progression remains unclear.					
32454127	7	40	theme	low	1140:1142	arg1	fractions					1152:1160	low density fractions	1140:1160	low density fractions following ultracentrifugation	1140:1190	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	9	41	theme	rapid	1668:1672	arg1	conversion					1674:1683	the rapid conversion	1664:1683	the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease	1574:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	2	42	theme	aggregate	362:370	arg1	pathway					381:387	the aggregate assembly pathway	358:387	the aggregate assembly pathway	358:387	In vivo pathogenesis studies have suggested that the presence of post-translational modifications can shift the aggregate assembly pathway and profoundly alter the disease phenotype.					
32454127	8	43	theme	PrP	1478:1480	arg1	assembly					1482:1489	PrP assembly	1478:1489	PrP assembly	1478:1489	Collectively, these results suggest that highly glycosylated PrP primarily converts as a GPI-anchored glycoform, with low involvement of HS co-factors, limiting PrP assembly mainly to oligomers.					
32454127	5	44	theme	prion	882:886	arg1	mobility					888:895	high prion mobility	877:895	high prion mobility	877:895	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	0	45	theme	post-translational	14:31	arg1	modifications					33:45	Prion protein post-translational modifications	0:45	Prion protein post-translational modifications	0:45	Prion protein post-translational modifications modulate heparan sulfate binding and limit aggregate size in prion disease.					
32454127	4	46	theme	disease	607:613	arg1	progression					615:625	disease progression	607:625	disease progression	607:625	However, the relevance of the two glycans to aggregate structure and disease progression remains unclear.					
32454127	5	47	theme	new	757:759	arg1	deposits					773:780	new plaque-like deposits	757:780	new plaque-like deposits	757:780	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	0	48	from	size	100:103	arg1	disease					114:120	prion disease	108:120	prion disease	108:120	Prion protein post-translational modifications modulate heparan sulfate binding and limit aggregate size in prion disease.					
32454127	8	49	theme	HS	1454:1455	arg1	co-factors					1457:1466	HS co-factors	1454:1466	HS co-factors	1454:1466	Collectively, these results suggest that highly glycosylated PrP primarily converts as a GPI-anchored glycoform, with low involvement of HS co-factors, limiting PrP assembly mainly to oligomers.					
32454127	9	50	theme	synaptic	1605:1612	arg1	deposits					1631:1638	diffuse, synaptic, and plaque-like deposits	1596:1638	diffuse, synaptic, and plaque-like deposits in the brain	1596:1651	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	5	51	theme	mobility	888:895	arg1	suggestive					863:872	suggestive	863:872	suggestive	863:872	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	0	52	theme	heparan	56:62	arg1	sulfate					64:70	heparan sulfate	56:70	heparan sulfate binding	56:78	Prion protein post-translational modifications modulate heparan sulfate binding and limit aggregate size in prion disease.					
32454127	8	53	theme	GPI-anchored	1406:1417	arg1	glycoform					1419:1427	a GPI-anchored glycoform	1404:1427	a GPI-anchored glycoform	1404:1427	Collectively, these results suggest that highly glycosylated PrP primarily converts as a GPI-anchored glycoform, with low involvement of HS co-factors, limiting PrP assembly mainly to oligomers.					
32454127	5	54	theme	plaque-like	761:771	arg1	deposits					773:780	new plaque-like deposits	757:780	new plaque-like deposits	757:780	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	6	55	dep	composed	1001:1008	arg1	uncleaved					1026:1034	uncleaved	1026:1034	uncleaved PrP	1026:1038	These plaque-like deposits were largely non-congophilic and composed of full length, uncleaved PrP, indicating retention of the glycophosphatidylinositol (GPI) anchor.					
32454127	3	56	link	N-linked	455:462	arg1	glycans					464:470	the N-linked glycans	451:470	the N-linked glycans	451:470	In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.					
32454127	9	57	gly	glycosylated	1533:1544	arg1	PrPC					1518:1521	PrPC	1518:1521	PrPC	1518:1521	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	5	58	theme	transit	901:907	arg1	suggestive					863:872	suggestive	863:872	suggestive	863:872	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	3	59	theme	N-linked	455:462	arg1	glycans					464:470	the N-linked glycans	451:470	the N-linked glycans	451:470	In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.					
32454127	6	60	theme	full	1013:1016	arg1	length					1018:1023	full length	1013:1023	full length	1013:1023	These plaque-like deposits were largely non-congophilic and composed of full length, uncleaved PrP, indicating retention of the glycophosphatidylinositol (GPI) anchor.					
32454127	7	61	theme	Prion	1109:1113	arg1	aggregates					1115:1124	Prion aggregates	1109:1124	Prion aggregates	1109:1124	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	7	62	with	consistent	1193:1202	arg1	oligomers					1209:1217	oligomers	1209:1217	oligomers	1209:1217	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	1	63	theme	Many	123:126	arg1	proteins					146:153	Many aggregation-prone proteins	123:153	Many aggregation-prone proteins linked to neurodegenerative disease	123:189	Many aggregation-prone proteins linked to neurodegenerative disease are post-translationally modified during their biogenesis.					
32454127	0	64	theme	sulfate	64:70	arg1	binding					72:78	heparan sulfate binding	56:78	heparan sulfate binding	56:78	Prion protein post-translational modifications modulate heparan sulfate binding and limit aggregate size in prion disease.					
32454127	5	65	theme	tri-glycosylated	727:742	arg1	PrP					744:746	tri-glycosylated PrP	727:746	tri-glycosylated PrP	727:746	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	5	65	theme	tri-glycosylated	727:742	arg1	glycan					719:724	an additional PrP glycan	701:724	an additional PrP glycan (tri-glycosylated PrP)	701:747	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	2	66	theme	pathogenesis	258:269	arg1	studies					271:277	In vivo pathogenesis studies	250:277	In vivo pathogenesis studies	250:277	In vivo pathogenesis studies have suggested that the presence of post-translational modifications can shift the aggregate assembly pathway and profoundly alter the disease phenotype.					
32454127	1	67	theme	aggregation-prone	128:144	arg1	proteins					146:153	Many aggregation-prone proteins	123:153	Many aggregation-prone proteins linked to neurodegenerative disease	123:189	Many aggregation-prone proteins linked to neurodegenerative disease are post-translationally modified during their biogenesis.					
32454127	9	68	from	brain	1647:1651	arg1	frequency					1583:1591	the high frequency	1574:1591	the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease	1574:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	9	68	from	brain	1647:1651	arg1	conversion					1674:1683	the rapid conversion	1664:1683	the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease	1574:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	4	69	theme	glycans	572:578	arg1	relevance					551:559	the relevance	547:559	the relevance of the two glycans to aggregate structure and disease progression	547:625	However, the relevance of the two glycans to aggregate structure and disease progression remains unclear.					
32454127	2	70	theme	In	250:251	arg1	studies					271:277	In vivo pathogenesis studies	250:277	In vivo pathogenesis studies	250:277	In vivo pathogenesis studies have suggested that the presence of post-translational modifications can shift the aggregate assembly pathway and profoundly alter the disease phenotype.					
32454127	3	71	theme	fibril	521:526	arg1	assembly					528:535	fibril assembly	521:535	fibril assembly	521:535	In prion disease, the N-linked glycans and GPI-anchor on the prion protein (PrP) impair fibril assembly.					
32454127	8	72	theme	low	1435:1437	arg1	involvement					1439:1449	low involvement	1435:1449	low involvement of HS co-factors	1435:1466	Collectively, these results suggest that highly glycosylated PrP primarily converts as a GPI-anchored glycoform, with low involvement of HS co-factors, limiting PrP assembly mainly to oligomers.					
32454127	9	73	theme	diffuse	1596:1602	arg1	deposits					1631:1638	diffuse, synaptic, and plaque-like deposits	1596:1638	diffuse, synaptic, and plaque-like deposits in the brain	1596:1651	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	9	74	theme	animal	1716:1721	arg1	disease					1729:1735	human and animal prion disease	1706:1735	human and animal prion disease	1706:1735	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	6	75	theme	GPI	1096:1098	arg1	anchor					1101:1106	the glycophosphatidylinositol (GPI) anchor	1065:1106	the glycophosphatidylinositol (GPI) anchor	1065:1106	These plaque-like deposits were largely non-congophilic and composed of full length, uncleaved PrP, indicating retention of the glycophosphatidylinositol (GPI) anchor.					
32454127	7	76	theme	GPI-anchored	1296:1307	arg1	prions					1309:1314	other predominantly GPI-anchored prions	1276:1314	other predominantly GPI-anchored prions	1276:1314	Prion aggregates sedimented in low density fractions following ultracentrifugation, consistent with oligomers, and bound low levels of heparan sulfate (HS) similar to other predominantly GPI-anchored prions.					
32454127	6	77	theme	anchor	1101:1106	arg1	retention					1052:1060	retention	1052:1060	retention of the glycophosphatidylinositol (GPI) anchor	1052:1106	These plaque-like deposits were largely non-congophilic and composed of full length, uncleaved PrP, indicating retention of the glycophosphatidylinositol (GPI) anchor.					
32454127	5	78	gly	tri-glycosylated	727:742	arg1	PrP					744:746	tri-glycosylated PrP	727:746	tri-glycosylated PrP	727:746	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	5	78	gly	tri-glycosylated	727:742	arg1	glycan					719:724	an additional PrP glycan	701:724	an additional PrP glycan (tri-glycosylated PrP)	701:747	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	8	79	gly	glycosylated	1365:1376	arg1	PrP					1378:1380	highly glycosylated PrP	1358:1380	highly glycosylated PrP	1358:1380	Collectively, these results suggest that highly glycosylated PrP primarily converts as a GPI-anchored glycoform, with low involvement of HS co-factors, limiting PrP assembly mainly to oligomers.					
32454127	5	80	theme	high	877:880	arg1	mobility					888:895	high prion mobility	877:895	high prion mobility	877:895	Here we show that prion-infected knockin mice expressing an additional PrP glycan (tri-glycosylated PrP) develop new plaque-like deposits on neuronal cell membranes, along the subarachnoid space, and periventricularly, suggestive of high prion mobility and transit through the interstitial fluid.					
32454127	9	81	theme	plaque-like	1619:1629	arg1	deposits					1631:1638	diffuse, synaptic, and plaque-like deposits	1596:1638	diffuse, synaptic, and plaque-like deposits in the brain	1596:1651	Since PrPC is highly glycosylated, these findings may explain the high frequency of diffuse, synaptic, and plaque-like deposits in the brain as well as the rapid conversion commonly observed in human and animal prion disease.					
32454127	1	82	theme	neurodegenerative	165:181	arg1	disease					183:189	neurodegenerative disease	165:189	neurodegenerative disease	165:189	Many aggregation-prone proteins linked to neurodegenerative disease are post-translationally modified during their biogenesis.					
32454127	2	83	dep	In	250:251	arg1	vivo					253:256	vivo	253:256	vivo	253:256	In vivo pathogenesis studies have suggested that the presence of post-translational modifications can shift the aggregate assembly pathway and profoundly alter the disease phenotype.					
32057848	0	0	theme	Maillard	97:104	arg1	reaction					106:113	Maillard reaction	97:113	Maillard reaction	97:113	Physicochemical properties and bioactivity of whey protein isolate-inulin conjugates obtained by Maillard reaction.					
32057848	4	1	from	bonds	598:602	arg1	FT-IR					607:611	FT-IR	607:611	FT-IR of conjugates compared with WPI	607:643	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	4	2	from	zone	518:521	arg1	bonds					598:602	new formed CN bonds	584:602	new formed CN bonds in FT-IR of conjugates compared with WPI	584:643	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	4	2	from	zone	518:521	arg1	content					572:578	decreased free amino acid content	546:578	decreased free amino acid content	546:578	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	4	2	from	zone	518:521	arg1	bonds					492:496	New bonds	488:496	New bonds at higher molecular zone appearing at SDS-PAGE	488:543	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	1	3	theme	protein	178:184	arg1	WPI					195:197	WPI	195:197	WPI	195:197	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	1	3	theme	protein	178:184	arg1	isolate					186:192	whey protein isolate	173:192	whey protein isolate (WPI)	173:198	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	7	4	theme	Emulsion	1032:1039	arg1	properties					1041:1050	Emulsion properties	1032:1050	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates	1032:1130	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	5	5	theme	time	747:750	arg1	increase					722:729	the increase	718:729	the increase of the reaction time	718:750	As the increase of the reaction time, both the brown intensity and fluorescence intensity of WPI-inulin conjugates became higher.					
32057848	1	6	theme	isolate	186:192	arg1	properties					131:140	functional properties	120:140	functional properties	120:140	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	1	6	theme	isolate	186:192	arg1	functions					160:168	physiological functions	146:168	physiological functions	146:168	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	4	7	theme	molecular	508:516	arg1	zone					518:521	higher molecular zone	501:521	higher molecular zone appearing at SDS-PAGE	501:543	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	5	8	dep	intensity	768:776	arg1	the					758:760	the	758:760	the	758:760	As the increase of the reaction time, both the brown intensity and fluorescence intensity of WPI-inulin conjugates became higher.					
32057848	8	9	theme	WPI	1359:1361	arg1	activities					1345:1354	the antioxidant activities	1329:1354	the antioxidant activities of WPI	1329:1361	AAPH+ scavenging test and ORAC measurement also revealed that covalent binding with inulin enhanced the antioxidant activities of WPI.					
32057848	4	10	theme	formed	588:593	arg1	bonds					598:602	new formed CN bonds	584:602	new formed CN bonds in FT-IR of conjugates compared with WPI	584:643	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	6	11	theme	dichroism	875:883	arg1	analysis					885:892	Circular dichroism analysis	866:892	Circular dichroism analysis	866:892	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	6	12	theme	surface	968:974	arg1	change					986:991	surface structure change	968:991	surface structure change	968:991	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	7	13	theme	conjugates	1121:1130	arg1	ES					1103:1104	ES	1103:1104	ES	1103:1104	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	7	13	theme	conjugates	1121:1130	arg1	EAI					1074:1076	EAI	1074:1076	EAI	1074:1076	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	7	13	theme	conjugates	1121:1130	arg1	activity					1064:1071	emulsion activity	1055:1071	emulsion activity (EAI)	1055:1077	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	7	13	theme	conjugates	1121:1130	arg1	stability					1092:1100	emulsion stability	1083:1100	emulsion stability (ES) of WPI-inulin conjugates	1083:1130	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	4	14	theme	bonds	685:689	arg1	formation					659:667	the formation	655:667	the formation of the covalent bonds between WPI and inulin	655:712	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	4	15	theme	new	584:586	arg1	bonds					598:602	new formed CN bonds	584:602	new formed CN bonds in FT-IR of conjugates compared with WPI	584:643	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	4	16	theme	covalent	676:683	arg1	bonds					685:689	the covalent bonds	672:689	the covalent bonds	672:689	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	6	17	theme	Circular	866:873	arg1	dichroism					875:883	Circular dichroism	866:883	Circular dichroism analysis	866:892	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	4	18	from	bonds	492:496	arg1	FT-IR					607:611	FT-IR	607:611	FT-IR of conjugates compared with WPI	607:643	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	8	19	theme	ORAC	1255:1258	arg1	measurement					1260:1270	ORAC measurement	1255:1270	ORAC measurement	1255:1270	AAPH+ scavenging test and ORAC measurement also revealed that covalent binding with inulin enhanced the antioxidant activities of WPI.					
32057848	1	20	dep	properties	131:140	arg1	The					116:118	The	116:118	The	116:118	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	9	21	theme	functional	1438:1447	arg1	properties					1449:1458	the functional properties	1434:1458	the functional properties of WPI	1434:1465	This work presented the conjugation with inulin successfully enhanced the functional properties of WPI.					
32057848	4	22	from	content	572:578	arg1	FT-IR					607:611	FT-IR	607:611	FT-IR of conjugates compared with WPI	607:643	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	7	23	theme	emulsion	1083:1090	arg1	ES					1103:1104	ES	1103:1104	ES	1103:1104	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	7	23	theme	emulsion	1083:1090	arg1	stability					1092:1100	emulsion stability	1083:1100	emulsion stability (ES) of WPI-inulin conjugates	1083:1130	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	4	24	theme	New	488:490	arg1	bonds					492:496	New bonds	488:496	New bonds at higher molecular zone appearing at SDS-PAGE	488:543	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	6	25	theme	secondary	944:952	arg1	structure					954:962	secondary structure	944:962	secondary structure	944:962	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	0	26	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and bioactivity of whey protein isolate-inulin conjugates obtained by Maillard reaction.					
32057848	4	27	theme	free	556:559	arg1	content					572:578	decreased free amino acid content	546:578	decreased free amino acid content	546:578	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	2	28	theme	effective	318:326	arg1	reaction					256:263	The Maillard reaction	243:263	The Maillard reaction covalently binding polysaccharides to proteins	243:310	The Maillard reaction covalently binding polysaccharides to proteins is an effective method to improve the functional activities of proteins.					
32057848	2	28	theme	effective	318:326	arg1	method					328:333	an effective method	315:333	an effective method to improve the functional activities of proteins	315:382	The Maillard reaction covalently binding polysaccharides to proteins is an effective method to improve the functional activities of proteins.					
32057848	8	29	theme	AAPH+	1229:1233	arg1	test					1246:1249	AAPH+ scavenging test	1229:1249	AAPH+ scavenging test	1229:1249	AAPH+ scavenging test and ORAC measurement also revealed that covalent binding with inulin enhanced the antioxidant activities of WPI.					
32057848	6	30	theme	Amino	845:849	arg1	contents					856:863	Amino acid contents	845:863	Amino acid contents	845:863	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	3	31	theme	WPI-inulin	385:394	arg1	conjugates					396:405	WPI-inulin conjugates	385:405	WPI-inulin conjugates	385:405	WPI-inulin conjugates were prepared by wet-heating method at 70 °C for 2 h, 4 h and 6 h, respectively.					
32057848	4	32	theme	decreased	546:554	arg1	content					572:578	decreased free amino acid content	546:578	decreased free amino acid content	546:578	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	7	33	theme	activity	1064:1071	arg1	properties					1041:1050	Emulsion properties	1032:1050	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates	1032:1130	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	5	34	theme	reaction	738:745	arg1	time					747:750	the reaction time	734:750	the reaction time	734:750	As the increase of the reaction time, both the brown intensity and fluorescence intensity of WPI-inulin conjugates became higher.					
32057848	4	35	theme	conjugates	616:625	arg1	FT-IR					607:611	FT-IR	607:611	FT-IR of conjugates compared with WPI	607:643	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	4	36	theme	acid	567:570	arg1	content					572:578	decreased free amino acid content	546:578	decreased free amino acid content	546:578	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	8	37	theme	antioxidant	1333:1343	arg1	activities					1345:1354	the antioxidant activities	1329:1354	the antioxidant activities of WPI	1329:1361	AAPH+ scavenging test and ORAC measurement also revealed that covalent binding with inulin enhanced the antioxidant activities of WPI.					
32057848	6	38	theme	acid	851:854	arg1	contents					856:863	Amino acid contents	845:863	Amino acid contents	845:863	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	1	39	theme	functional	120:129	arg1	properties					131:140	functional properties	120:140	functional properties	120:140	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	6	40	theme	SEM	898:900	arg1	analysis					902:909	SEM analysis	898:909	SEM analysis	898:909	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	5	41	theme	WPI-inulin	808:817	arg1	conjugates					819:828	WPI-inulin conjugates	808:828	WPI-inulin conjugates	808:828	As the increase of the reaction time, both the brown intensity and fluorescence intensity of WPI-inulin conjugates became higher.					
32057848	0	42	theme	whey	46:49	arg1	conjugates					74:83	whey protein isolate-inulin conjugates	46:83	whey protein isolate-inulin conjugates	46:83	Physicochemical properties and bioactivity of whey protein isolate-inulin conjugates obtained by Maillard reaction.					
32057848	4	43	theme	amino	561:565	arg1	content					572:578	decreased free amino acid content	546:578	decreased free amino acid content	546:578	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	5	44	theme	conjugates	819:828	arg1	intensity					768:776	brown intensity	762:776	brown intensity	762:776	As the increase of the reaction time, both the brown intensity and fluorescence intensity of WPI-inulin conjugates became higher.					
32057848	5	44	theme	conjugates	819:828	arg1	intensity					795:803	fluorescence intensity	782:803	fluorescence intensity	782:803	As the increase of the reaction time, both the brown intensity and fluorescence intensity of WPI-inulin conjugates became higher.					
32057848	1	45	theme	isoelectric	219:229	arg1	PI					238:239	PI	238:239	PI	238:239	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	1	45	theme	isoelectric	219:229	arg1	point					231:235	its isoelectric point	215:235	its isoelectric point (PI)	215:240	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	7	46	theme	emulsion	1055:1062	arg1	EAI					1074:1076	EAI	1074:1076	EAI	1074:1076	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	7	46	theme	emulsion	1055:1062	arg1	activity					1064:1071	emulsion activity	1055:1071	emulsion activity (EAI)	1055:1077	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	6	47	dep	structure	933:941	arg1	the					921:923	the	921:923	the	921:923	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	5	48	theme	fluorescence	782:793	arg1	intensity					795:803	fluorescence intensity	782:803	fluorescence intensity	782:803	As the increase of the reaction time, both the brown intensity and fluorescence intensity of WPI-inulin conjugates became higher.					
32057848	0	49	theme	isolate-inulin	59:72	arg1	conjugates					74:83	whey protein isolate-inulin conjugates	46:83	whey protein isolate-inulin conjugates	46:83	Physicochemical properties and bioactivity of whey protein isolate-inulin conjugates obtained by Maillard reaction.					
32057848	6	50	theme	primary	925:931	arg1	structure					933:941	primary structure	925:941	primary structure	925:941	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	5	51	theme	brown	762:766	arg1	intensity					768:776	brown intensity	762:776	brown intensity	762:776	As the increase of the reaction time, both the brown intensity and fluorescence intensity of WPI-inulin conjugates became higher.					
32057848	2	52	theme	functional	350:359	arg1	activities					361:370	the functional activities	346:370	the functional activities of proteins	346:382	The Maillard reaction covalently binding polysaccharides to proteins is an effective method to improve the functional activities of proteins.					
32057848	0	53	theme	protein	51:57	arg1	conjugates					74:83	whey protein isolate-inulin conjugates	46:83	whey protein isolate-inulin conjugates	46:83	Physicochemical properties and bioactivity of whey protein isolate-inulin conjugates obtained by Maillard reaction.					
32057848	6	54	with	covalent	1010:1017	arg1	inulin					1024:1029	inulin	1024:1029	inulin	1024:1029	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	9	55	theme	WPI	1463:1465	arg1	properties					1449:1458	the functional properties	1434:1458	the functional properties of WPI	1434:1465	This work presented the conjugation with inulin successfully enhanced the functional properties of WPI.					
32057848	7	56	theme	stability	1092:1100	arg1	properties					1041:1050	Emulsion properties	1032:1050	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates	1032:1130	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	7	57	dep	showed	1155:1160	arg1	enhanced					1176:1183	enhanced	1176:1183	showed significantly enhanced compared with WPI at range of pH 3 to pH 7	1155:1226	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	8	58	with	binding	1300:1306	arg1	inulin					1313:1318	inulin	1313:1318	inulin	1313:1318	AAPH+ scavenging test and ORAC measurement also revealed that covalent binding with inulin enhanced the antioxidant activities of WPI.					
32057848	7	59	theme	WPI-inulin	1110:1119	arg1	conjugates					1121:1130	WPI-inulin conjugates	1110:1130	WPI-inulin conjugates	1110:1130	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	1	60	theme	physiological	146:158	arg1	functions					160:168	physiological functions	146:168	physiological functions	146:168	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	7	61	dep	pH 7	1223:1226	arg1	to					1220:1221	to	1220:1221	to	1220:1221	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	6	62	theme	structure	976:984	arg1	change					986:991	surface structure change	968:991	surface structure change	968:991	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	7	63	from	range	1206:1210	arg1	WPI					1199:1201	WPI	1199:1201	WPI at range of pH 3 to pH 7	1199:1226	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	8	64	theme	covalent	1291:1298	arg1	binding					1300:1306	covalent binding	1291:1306	covalent binding with inulin	1291:1318	AAPH+ scavenging test and ORAC measurement also revealed that covalent binding with inulin enhanced the antioxidant activities of WPI.					
32057848	3	65	from	70 °C	446:450	arg1	method					436:441	method	436:441	method at 70 °C for 2 h, 4 h and 6 h, respectively	436:485	WPI-inulin conjugates were prepared by wet-heating method at 70 °C for 2 h, 4 h and 6 h, respectively.					
32057848	1	66	theme	whey	173:176	arg1	WPI					195:197	WPI	195:197	WPI	195:197	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	1	66	theme	whey	173:176	arg1	isolate					186:192	whey protein isolate	173:192	whey protein isolate (WPI)	173:198	The functional properties and physiological functions of whey protein isolate (WPI) decreased near its isoelectric point (PI).					
32057848	4	67	theme	CN	595:596	arg1	bonds					598:602	new formed CN bonds	584:602	new formed CN bonds in FT-IR of conjugates compared with WPI	584:643	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	2	68	theme	Maillard	247:254	arg1	reaction					256:263	The Maillard reaction	243:263	The Maillard reaction covalently binding polysaccharides to proteins	243:310	The Maillard reaction covalently binding polysaccharides to proteins is an effective method to improve the functional activities of proteins.					
32057848	2	68	theme	Maillard	247:254	arg1	method					328:333	an effective method	315:333	an effective method to improve the functional activities of proteins	315:382	The Maillard reaction covalently binding polysaccharides to proteins is an effective method to improve the functional activities of proteins.					
32057848	8	69	theme	scavenging	1235:1244	arg1	test					1246:1249	AAPH+ scavenging test	1229:1249	AAPH+ scavenging test	1229:1249	AAPH+ scavenging test and ORAC measurement also revealed that covalent binding with inulin enhanced the antioxidant activities of WPI.					
32057848	7	70	theme	pH 7	1223:1226	arg1	range					1206:1210	range	1206:1210	range of pH 3 to pH 7	1206:1226	Emulsion properties of emulsion activity (EAI) and emulsion stability (ES) of WPI-inulin conjugates were assessed and both showed significantly enhanced compared with WPI at range of pH 3 to pH 7.					
32057848	0	71	theme	conjugates	74:83	arg1	bioactivity					31:41	bioactivity	31:41	bioactivity	31:41	Physicochemical properties and bioactivity of whey protein isolate-inulin conjugates obtained by Maillard reaction.					
32057848	0	71	theme	conjugates	74:83	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and bioactivity of whey protein isolate-inulin conjugates obtained by Maillard reaction.					
32057848	4	72	theme	higher	501:506	arg1	zone					518:521	higher molecular zone	501:521	higher molecular zone appearing at SDS-PAGE	501:543	New bonds at higher molecular zone appearing at SDS-PAGE, decreased free amino acid content and new formed CN bonds in FT-IR of conjugates compared with WPI confirmed the formation of the covalent bonds between WPI and inulin.					
32057848	2	73	theme	proteins	375:382	arg1	activities					361:370	the functional activities	346:370	the functional activities of proteins	346:382	The Maillard reaction covalently binding polysaccharides to proteins is an effective method to improve the functional activities of proteins.					
32057848	6	74	theme	protein	996:1002	arg1	change					986:991	surface structure change	968:991	surface structure change	968:991	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	6	74	theme	protein	996:1002	arg1	structure					933:941	primary structure	925:941	primary structure	925:941	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
32057848	6	74	theme	protein	996:1002	arg1	structure					954:962	secondary structure	944:962	secondary structure	944:962	Amino acid contents, Circular dichroism analysis and SEM analysis presented the primary structure, secondary structure and surface structure change of protein after covalent with inulin.					
34069622	1	0	with	polyanions	224:233	arg1	hyaluronate					346:356	sodium hyaluronate	339:356	sodium hyaluronate (HA)	339:361	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	0	with	polyanions	224:233	arg1	cellulose					295:303	sodium sulfoethyl cellulose	277:303	sodium sulfoethyl cellulose (SEC)	277:309	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	0	with	polyanions	224:233	arg1	functionalities					250:264	different functionalities	240:264	different functionalities	240:264	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	0	with	polyanions	224:233	arg1	alginate					319:326	sodium alginate	312:326	sodium alginate (ALG)	312:332	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	9	1	theme	intermediate	1793:1804	arg1	response					1806:1813	an intermediate response	1790:1813	an intermediate response	1790:1813	The CS/HA composite showed an intermediate response.					
34069622	6	2	theme	CS	1400:1401	arg1	weaker					1423:1428	weaker	1423:1428	weaker	1423:1428	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	2	theme	CS	1400:1401	arg1	crystallization					1381:1395	the crystallization	1377:1395	the crystallization of CS in the PEC layer	1377:1418	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	4	3	theme	CS	1046:1047	arg1	decomposition					1017:1029	the thermal decomposition	1005:1029	the thermal decomposition of crystalline CS in the PEC composition	1005:1070	Thermogravimetric analysis of the CS/SEC film registers a high-temperature peak associated with the thermal decomposition of crystalline CS in the PEC composition.					
34069622	10	4	theme	layer	1844:1848	arg1	effect					1826:1831	the effect	1822:1831	the effect of the PEC layer on the properties of the poly-layer composites	1822:1895	Thus, the effect of the PEC layer on the properties of the poly-layer composites decreases in the order CS/SEC > CS/HA > CS/ALG.					
34069622	1	5	theme	sodium	277:282	arg1	SEC					306:308	SEC	306:308	SEC	306:308	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	5	theme	sodium	277:282	arg1	cellulose					295:303	sodium sulfoethyl cellulose	277:303	sodium sulfoethyl cellulose (SEC)	277:309	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	2	6	theme	state	593:597	arg1	transformation					556:569	the transformation	552:569	the transformation of the CS polymorphic state	552:597	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	6	7	theme	thermal	1451:1457	arg1	degradation					1459:1469	the thermal degradation	1447:1469	the thermal degradation of these films	1447:1484	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	1	8	theme	composites	186:195	arg1	properties					161:170	the thermal and structural properties	134:170	the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA)	134:361	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	2	9	theme	CS	578:579	arg1	state					593:597	the CS polymorphic state	574:597	the CS polymorphic state	574:597	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	4	10	from	decomposition	1017:1029	arg1	composition					1060:1070	the PEC composition	1052:1070	the PEC composition	1052:1070	Thermogravimetric analysis of the CS/SEC film registers a high-temperature peak associated with the thermal decomposition of crystalline CS in the PEC composition.					
34069622	8	11	theme	composite	1640:1648	arg1	analysis					1617:1624	Dynamic mechanical analysis	1598:1624	Dynamic mechanical analysis of the CS/ALG composite	1598:1648	Dynamic mechanical analysis of the CS/ALG composite showed two glass transition temperatures close to those of the original polymers, indicating weak PEC formation.					
34069622	10	12	from	effect	1826:1831	arg1	properties					1857:1866	the properties	1853:1866	the properties of the poly-layer composites	1853:1895	Thus, the effect of the PEC layer on the properties of the poly-layer composites decreases in the order CS/SEC > CS/HA > CS/ALG.					
34069622	3	13	theme	diffraction	700:710	arg1	analysis					712:719	X-ray diffraction analysis	694:719	X-ray diffraction analysis	694:719	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	7	14	theme	CS/HA	1555:1559	arg1	decomposition					1538:1550	the thermal decomposition	1526:1550	the thermal decomposition of CS/HA	1526:1559	A high-temperature peak is recorded in the thermal decomposition of CS/HA and is absent in the case of CS/ALG.					
34069622	2	15	theme	deposition	492:501	arg1	process					466:472	a process	464:472	a process of layer-by-layer deposition of oppositely charged polymers	464:532	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	8	16	theme	Dynamic	1598:1604	arg1	analysis					1617:1624	Dynamic mechanical analysis	1598:1624	Dynamic mechanical analysis of the CS/ALG composite	1598:1648	Dynamic mechanical analysis of the CS/ALG composite showed two glass transition temperatures close to those of the original polymers, indicating weak PEC formation.					
34069622	7	17	from	absent	1568:1573	arg1	case					1582:1585	the case	1578:1585	the case of CS/ALG	1578:1595	A high-temperature peak is recorded in the thermal decomposition of CS/HA and is absent in the case of CS/ALG.					
34069622	6	18	from	crystallization	1381:1395	arg1	layer					1414:1418	the PEC layer	1406:1418	the PEC layer	1406:1418	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	8	19	theme	transition	1667:1676	arg1	temperatures					1678:1689	two glass transition temperatures	1657:1689	two glass transition temperatures close to those of the original polymers	1657:1729	Dynamic mechanical analysis of the CS/ALG composite showed two glass transition temperatures close to those of the original polymers, indicating weak PEC formation.					
34069622	10	20	theme	poly-layer	1875:1884	arg1	composites					1886:1895	the poly-layer composites	1871:1895	the poly-layer composites	1871:1895	Thus, the effect of the PEC layer on the properties of the poly-layer composites decreases in the order CS/SEC > CS/HA > CS/ALG.					
34069622	2	21	dep	relaxation	621:630	arg1	the					617:619	the	617:619	the	617:619	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	2	22	theme	polymers	525:532	arg1	deposition					492:501	layer-by-layer deposition	477:501	layer-by-layer deposition of oppositely charged polymers	477:532	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	10	23	theme	CS/SEC	1920:1925	arg1	CS/ALG					1937:1942	the order CS/SEC > CS/HA > CS/ALG	1910:1942	the order CS/SEC > CS/HA > CS/ALG	1910:1942	Thus, the effect of the PEC layer on the properties of the poly-layer composites decreases in the order CS/SEC > CS/HA > CS/ALG.					
34069622	1	24	theme	thermal	138:144	arg1	properties					161:170	the thermal and structural properties	134:170	the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA)	134:361	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	4	25	theme	CS/SEC	943:948	arg1	film					950:953	the CS/SEC film	939:953	the CS/SEC film	939:953	Thermogravimetric analysis of the CS/SEC film registers a high-temperature peak associated with the thermal decomposition of crystalline CS in the PEC composition.					
34069622	5	26	theme	strong	1269:1274	arg1	salt					1281:1284	a strong acid salt	1267:1284	a strong acid salt	1267:1284	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	5	26	theme	strong	1269:1274	arg1	SEC					1262:1264	SEC	1262:1264	SEC (a strong acid salt)	1262:1285	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	3	27	theme	PEC	754:756	arg1	layer					758:762	the PEC layer	750:762	the PEC layer	750:762	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	6	28	theme	polyacid	1348:1355	arg1	HA					1372:1373	HA	1372:1373	HA	1372:1373	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	28	theme	polyacid	1348:1355	arg1	ALG					1364:1366	ALG	1364:1366	ALG	1364:1366	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	28	theme	polyacid	1348:1355	arg1	salts					1357:1361	weak polyacid salts	1343:1361	weak polyacid salts (ALG and HA)	1343:1374	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	5	29	theme	mechanical	1098:1107	arg1	analysis					1109:1116	the dynamic mechanical analysis	1086:1116	the dynamic mechanical analysis	1086:1116	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	6	30	with	composites	1327:1336	arg1	HA					1372:1373	HA	1372:1373	HA	1372:1373	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	30	with	composites	1327:1336	arg1	ALG					1364:1366	ALG	1364:1366	ALG	1364:1366	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	30	with	composites	1327:1336	arg1	salts					1357:1361	weak polyacid salts	1343:1361	weak polyacid salts (ALG and HA)	1343:1374	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	1	31	theme	structural	150:159	arg1	properties					161:170	the thermal and structural properties	134:170	the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA)	134:361	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	3	32	theme	chains	838:843	arg1	crystallization					812:826	crystallization	812:826	crystallization of the CS chains	812:843	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	3	32	theme	chains	838:843	arg1	formation					853:861	the formation	849:861	the formation of a predominantly anhydrous CS modification	849:906	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	2	33	theme	multilayer	672:681	arg1	composite					683:691	the resulting multilayer composite	658:691	the resulting multilayer composite	658:691	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	4	34	theme	Thermogravimetric	909:925	arg1	analysis					927:934	Thermogravimetric analysis	909:934	Thermogravimetric analysis of the CS/SEC film	909:953	Thermogravimetric analysis of the CS/SEC film registers a high-temperature peak associated with the thermal decomposition of crystalline CS in the PEC composition.					
34069622	3	35	theme	CS/SEC	771:776	arg1	film					789:792	the CS/SEC multilayer film	767:792	the CS/SEC multilayer film	767:792	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	0	36	theme	Anionic	85:91	arg1	Polysaccharides					93:107	Anionic Polysaccharides	85:107	Anionic Polysaccharides	85:107	Thermal Properties and Structural Features of Multilayer Films Based on Chitosan and Anionic Polysaccharides.					
34069622	8	37	theme	PEC	1748:1750	arg1	formation					1752:1760	weak PEC formation	1743:1760	weak PEC formation	1743:1760	Dynamic mechanical analysis of the CS/ALG composite showed two glass transition temperatures close to those of the original polymers, indicating weak PEC formation.					
34069622	2	38	theme	polymer	416:422	arg1	mixing					424:429	polymer mixing	416:429	polymer mixing	416:429	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	2	39	theme	layer	455:459	arg1	formation					436:444	the formation	432:444	the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers	432:532	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	10	40	theme	PEC	1840:1842	arg1	layer					1844:1848	the PEC layer	1836:1848	the PEC layer	1836:1848	Thus, the effect of the PEC layer on the properties of the poly-layer composites decreases in the order CS/SEC > CS/HA > CS/ALG.					
34069622	0	41	theme	Thermal	0:6	arg1	Properties					8:17	Thermal Properties	0:17	Thermal Properties	0:17	Thermal Properties and Structural Features of Multilayer Films Based on Chitosan and Anionic Polysaccharides.					
34069622	1	42	theme	sulfoethyl	284:293	arg1	SEC					306:308	SEC	306:308	SEC	306:308	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	42	theme	sulfoethyl	284:293	arg1	cellulose					295:303	sodium sulfoethyl cellulose	277:303	sodium sulfoethyl cellulose (SEC)	277:309	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	3	43	theme	CS	892:893	arg1	modification					895:906	a predominantly anhydrous CS modification	866:906	a predominantly anhydrous CS modification	866:906	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	5	44	theme	single	1163:1168	arg1	transition					1176:1185	a single glass transition	1161:1185	a single glass transition temperature	1161:1197	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	4	45	theme	high-temperature	967:982	arg1	peak					984:987	a high-temperature peak	965:987	a high-temperature peak associated with the thermal decomposition of crystalline CS in the PEC composition	965:1070	Thermogravimetric analysis of the CS/SEC film registers a high-temperature peak associated with the thermal decomposition of crystalline CS in the PEC composition.					
34069622	5	46	theme	CS/SEC	1123:1128	arg1	composite					1130:1138	the CS/SEC composite	1119:1138	the CS/SEC composite	1119:1138	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	0	47	theme	Multilayer	46:55	arg1	Films					57:61	Multilayer Films	46:61	Multilayer Films	46:61	Thermal Properties and Structural Features of Multilayer Films Based on Chitosan and Anionic Polysaccharides.					
34069622	1	48	theme	sodium	312:317	arg1	ALG					329:331	ALG	329:331	ALG	329:331	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	48	theme	sodium	312:317	arg1	alginate					319:326	sodium alginate	312:326	sodium alginate (ALG)	312:332	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	4	49	theme	PEC	1056:1058	arg1	composition					1060:1070	the PEC composition	1052:1070	the PEC composition	1052:1070	Thermogravimetric analysis of the CS/SEC film registers a high-temperature peak associated with the thermal decomposition of crystalline CS in the PEC composition.					
34069622	6	50	theme	films	1480:1484	arg1	degradation					1459:1469	the thermal degradation	1447:1469	the thermal degradation of these films	1447:1484	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	51	theme	PEC	1410:1412	arg1	layer					1414:1418	the PEC layer	1406:1418	the PEC layer	1406:1418	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	3	52	theme	layer	758:762	arg1	formation					737:745	the formation	733:745	the formation of the PEC layer in the CS/SEC multilayer film	733:792	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	4	53	theme	crystalline	1034:1044	arg1	CS					1046:1047	crystalline CS	1034:1047	crystalline CS	1034:1047	Thermogravimetric analysis of the CS/SEC film registers a high-temperature peak associated with the thermal decomposition of crystalline CS in the PEC composition.					
34069622	5	54	theme	transition	1176:1185	arg1	temperature					1187:1197	a single glass transition temperature	1161:1197	a single glass transition temperature	1161:1197	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	3	55	theme	X-ray	694:698	arg1	analysis					712:719	X-ray diffraction analysis	694:719	X-ray diffraction analysis	694:719	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	8	56	theme	mechanical	1606:1615	arg1	analysis					1617:1624	Dynamic mechanical analysis	1598:1624	Dynamic mechanical analysis of the CS/ALG composite	1598:1648	Dynamic mechanical analysis of the CS/ALG composite showed two glass transition temperatures close to those of the original polymers, indicating weak PEC formation.					
34069622	1	57	theme	multilayer	175:184	arg1	composites					186:195	multilayer composites	175:195	multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA)	175:361	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	2	58	theme	polymorphic	581:591	arg1	state					593:597	the CS polymorphic state	574:597	the CS polymorphic state	574:597	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	8	59	theme	CS/ALG	1633:1638	arg1	composite					1640:1648	the CS/ALG composite	1629:1648	the CS/ALG composite	1629:1648	Dynamic mechanical analysis of the CS/ALG composite showed two glass transition temperatures close to those of the original polymers, indicating weak PEC formation.					
34069622	7	60	theme	thermal	1530:1536	arg1	decomposition					1538:1550	the thermal decomposition	1526:1550	the thermal decomposition of CS/HA	1526:1559	A high-temperature peak is recorded in the thermal decomposition of CS/HA and is absent in the case of CS/ALG.					
34069622	2	61	theme	layer-by-layer	477:490	arg1	deposition					492:501	layer-by-layer deposition	477:501	layer-by-layer deposition of oppositely charged polymers	477:532	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	2	62	theme	resulting	662:670	arg1	composite					683:691	the resulting multilayer composite	658:691	the resulting multilayer composite	658:691	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	10	63	theme	composites	1886:1895	arg1	properties					1857:1866	the properties	1853:1866	the properties of the poly-layer composites	1853:1895	Thus, the effect of the PEC layer on the properties of the poly-layer composites decreases in the order CS/SEC > CS/HA > CS/ALG.					
34069622	8	64	theme	glass	1661:1665	arg1	temperatures					1678:1689	two glass transition temperatures	1657:1689	two glass transition temperatures close to those of the original polymers	1657:1729	Dynamic mechanical analysis of the CS/ALG composite showed two glass transition temperatures close to those of the original polymers, indicating weak PEC formation.					
34069622	7	65	from	case	1582:1585	arg1	peak					1506:1509	A high-temperature peak	1487:1509	A high-temperature peak	1487:1509	A high-temperature peak is recorded in the thermal decomposition of CS/HA and is absent in the case of CS/ALG.					
34069622	7	65	from	case	1582:1585	arg1	absent					1568:1573	absent	1568:1573	absent	1568:1573	A high-temperature peak is recorded in the thermal decomposition of CS/HA and is absent in the case of CS/ALG.					
34069622	2	66	theme	charged	517:523	arg1	polymers					525:532	oppositely charged polymers	506:532	oppositely charged polymers	506:532	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	10	67	theme	order	1914:1918	arg1	CS/ALG					1937:1942	the order CS/SEC > CS/HA > CS/ALG	1910:1942	the order CS/SEC > CS/HA > CS/ALG	1910:1942	Thus, the effect of the PEC layer on the properties of the poly-layer composites decreases in the order CS/SEC > CS/HA > CS/ALG.					
34069622	7	68	theme	high-temperature	1489:1504	arg1	peak					1506:1509	A high-temperature peak	1487:1509	A high-temperature peak	1487:1509	A high-temperature peak is recorded in the thermal decomposition of CS/HA and is absent in the case of CS/ALG.					
34069622	7	68	theme	high-temperature	1489:1504	arg1	absent					1568:1573	absent	1568:1573	absent	1568:1573	A high-temperature peak is recorded in the thermal decomposition of CS/HA and is absent in the case of CS/ALG.					
34069622	2	69	theme	PEC	451:453	arg1	layer					455:459	a PEC layer	449:459	a PEC layer	449:459	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	2	70	theme	composite	683:691	arg1	relaxation					621:630	relaxation	621:630	relaxation	621:630	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	2	70	theme	composite	683:691	arg1	properties					644:653	thermal properties	636:653	thermal properties	636:653	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	6	71	theme	multilayer	1316:1325	arg1	composites					1327:1336	multilayer composites	1316:1336	multilayer composites with weak polyacid salts (ALG and HA)	1316:1374	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	72	theme	weak	1343:1346	arg1	HA					1372:1373	HA	1372:1373	HA	1372:1373	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	72	theme	weak	1343:1346	arg1	ALG					1364:1366	ALG	1364:1366	ALG	1364:1366	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	72	theme	weak	1343:1346	arg1	salts					1357:1361	weak polyacid salts	1343:1361	weak polyacid salts (ALG and HA)	1343:1374	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	4	73	theme	film	950:953	arg1	analysis					927:934	Thermogravimetric analysis	909:934	Thermogravimetric analysis of the CS/SEC film	909:953	Thermogravimetric analysis of the CS/SEC film registers a high-temperature peak associated with the thermal decomposition of crystalline CS in the PEC composition.					
34069622	3	74	from	formation	737:745	arg1	film					789:792	the CS/SEC multilayer film	767:792	the CS/SEC multilayer film	767:792	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	10	75	theme	>	1935:1935	arg1	CS/ALG					1937:1942	the order CS/SEC > CS/HA > CS/ALG	1910:1942	the order CS/SEC > CS/HA > CS/ALG	1910:1942	Thus, the effect of the PEC layer on the properties of the poly-layer composites decreases in the order CS/SEC > CS/HA > CS/ALG.					
34069622	8	76	theme	original	1713:1720	arg1	polymers					1722:1729	the original polymers	1709:1729	the original polymers	1709:1729	Dynamic mechanical analysis of the CS/ALG composite showed two glass transition temperatures close to those of the original polymers, indicating weak PEC formation.					
34069622	3	77	theme	CS	835:836	arg1	chains					838:843	the CS chains	831:843	the CS chains	831:843	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	3	78	theme	multilayer	778:787	arg1	film					789:792	the CS/SEC multilayer film	767:792	the CS/SEC multilayer film	767:792	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	1	79	theme	sodium	339:344	arg1	HA					359:360	HA	359:360	HA	359:360	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	79	theme	sodium	339:344	arg1	hyaluronate					346:356	sodium hyaluronate	339:356	sodium hyaluronate (HA)	339:361	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	10	80	theme	>	1927:1927	arg1	CS/ALG					1937:1942	the order CS/SEC > CS/HA > CS/ALG	1910:1942	the order CS/SEC > CS/HA > CS/ALG	1910:1942	Thus, the effect of the PEC layer on the properties of the poly-layer composites decreases in the order CS/SEC > CS/HA > CS/ALG.					
34069622	2	81	theme	thermal	636:642	arg1	properties					644:653	thermal properties	636:653	thermal properties	636:653	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	5	82	theme	dynamic	1090:1096	arg1	analysis					1109:1116	the dynamic mechanical analysis	1086:1116	the dynamic mechanical analysis	1086:1116	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	6	83	dep	salts	1357:1361	arg1	HA					1372:1373	HA	1372:1373	HA	1372:1373	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	83	dep	salts	1357:1361	arg1	ALG					1364:1366	ALG	1364:1366	ALG	1364:1366	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	6	83	dep	salts	1357:1361	arg1	salts					1357:1361	weak polyacid salts	1343:1361	weak polyacid salts (ALG and HA)	1343:1374	For multilayer composites with weak polyacid salts (ALG and HA), the crystallization of CS in the PEC layer is weaker, as reflected in the thermal degradation of these films.					
34069622	8	84	theme	weak	1743:1746	arg1	formation					1752:1760	weak PEC formation	1743:1760	weak PEC formation	1743:1760	Dynamic mechanical analysis of the CS/ALG composite showed two glass transition temperatures close to those of the original polymers, indicating weak PEC formation.					
34069622	7	85	theme	CS/ALG	1590:1595	arg1	case					1582:1585	the case	1578:1585	the case of CS/ALG	1578:1595	A high-temperature peak is recorded in the thermal decomposition of CS/HA and is absent in the case of CS/ALG.					
34069622	5	86	theme	acid	1276:1279	arg1	salt					1281:1284	a strong acid salt	1267:1284	a strong acid salt	1267:1284	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	5	86	theme	acid	1276:1279	arg1	SEC					1262:1264	SEC	1262:1264	SEC (a strong acid salt)	1262:1285	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	0	87	theme	Structural	23:32	arg1	Features					34:41	Structural Features	23:41	Structural Features	23:41	Thermal Properties and Structural Features of Multilayer Films Based on Chitosan and Anionic Polysaccharides.					
34069622	3	88	theme	modification	895:906	arg1	crystallization					812:826	crystallization	812:826	crystallization of the CS chains	812:843	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	3	88	theme	modification	895:906	arg1	formation					853:861	the formation	849:861	the formation of a predominantly anhydrous CS modification	849:906	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	4	89	theme	thermal	1009:1015	arg1	decomposition					1017:1029	the thermal decomposition	1005:1029	the thermal decomposition of crystalline CS in the PEC composition	1005:1070	Thermogravimetric analysis of the CS/SEC film registers a high-temperature peak associated with the thermal decomposition of crystalline CS in the PEC composition.					
34069622	5	90	theme	glass	1170:1174	arg1	transition					1176:1185	a single glass transition	1161:1185	a single glass transition temperature	1161:1197	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	1	91	with	chitosan	206:213	arg1	hyaluronate					346:356	sodium hyaluronate	339:356	sodium hyaluronate (HA)	339:361	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	91	with	chitosan	206:213	arg1	cellulose					295:303	sodium sulfoethyl cellulose	277:303	sodium sulfoethyl cellulose (SEC)	277:309	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	91	with	chitosan	206:213	arg1	functionalities					250:264	different functionalities	240:264	different functionalities	240:264	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	91	with	chitosan	206:213	arg1	alginate					319:326	sodium alginate	312:326	sodium alginate (ALG)	312:332	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	3	92	theme	anhydrous	882:890	arg1	modification					895:906	a predominantly anhydrous CS modification	866:906	a predominantly anhydrous CS modification	866:906	X-ray diffraction analysis showed that the formation of the PEC layer in the CS/SEC multilayer film is accompanied by crystallization of the CS chains and the formation of a predominantly anhydrous CS modification.					
34069622	10	93	theme	CS/HA	1929:1933	arg1	CS/ALG					1937:1942	the order CS/SEC > CS/HA > CS/ALG	1910:1942	the order CS/SEC > CS/HA > CS/ALG	1910:1942	Thus, the effect of the PEC layer on the properties of the poly-layer composites decreases in the order CS/SEC > CS/HA > CS/ALG.					
34069622	2	94	theme	polyelectrolyte	371:385	arg1	complexes					387:395	polyelectrolyte complexes	371:395	polyelectrolyte complexes (PECs) obtained by polymer mixing	371:429	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	2	94	theme	polyelectrolyte	371:385	arg1	PECs					398:401	PECs	398:401	PECs	398:401	Unlike polyelectrolyte complexes (PECs) obtained by polymer mixing, the formation of a PEC layer by a process of layer-by-layer deposition of oppositely charged polymers is accompanied by the transformation of the CS polymorphic state, and this affects the relaxation and thermal properties of the resulting multilayer composite.					
34069622	0	95	theme	Films	57:61	arg1	Properties					8:17	Thermal Properties	0:17	Thermal Properties	0:17	Thermal Properties and Structural Features of Multilayer Films Based on Chitosan and Anionic Polysaccharides.					
34069622	0	95	theme	Films	57:61	arg1	Features					34:41	Structural Features	23:41	Structural Features	23:41	Thermal Properties and Structural Features of Multilayer Films Based on Chitosan and Anionic Polysaccharides.					
34069622	9	96	theme	CS/HA	1767:1771	arg1	composite					1773:1781	The CS/HA composite	1763:1781	The CS/HA composite	1763:1781	The CS/HA composite showed an intermediate response.					
34069622	5	97	theme	strong	1213:1218	arg1	interaction					1220:1230	a strong interaction	1211:1230	a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS	1211:1309	According to the dynamic mechanical analysis, the CS/SEC composite was characterized by a single glass transition temperature, indicating a strong interaction between the layers when using SEC (a strong acid salt) as the counterion to CS.					
34069622	1	98	theme	different	240:248	arg1	hyaluronate					346:356	sodium hyaluronate	339:356	sodium hyaluronate (HA)	339:361	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	98	theme	different	240:248	arg1	cellulose					295:303	sodium sulfoethyl cellulose	277:303	sodium sulfoethyl cellulose (SEC)	277:309	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	98	theme	different	240:248	arg1	functionalities					250:264	different functionalities	240:264	different functionalities	240:264	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
34069622	1	98	theme	different	240:248	arg1	alginate					319:326	sodium alginate	312:326	sodium alginate (ALG)	312:332	This study investigates the thermal and structural properties of multilayer composites based on chitosan (CS) and polyanions with different functionalities, including sodium sulfoethyl cellulose (SEC), sodium alginate (ALG), and sodium hyaluronate (HA).					
33761173	6	0	theme	glycans	1052:1058	arg1	Characterization					1032:1047	Characterization	1032:1047	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	1	theme	Basic	1255:1259	arg1	Protocol					1261:1268	glycopeptide enrichment Basic Protocol 2	1231:1270	glycopeptide enrichment Basic Protocol 2	1231:1270	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	2	from	glycans	1052:1058	arg1	Protocol					1112:1119	purified glycoprotein Support Protocol 1	1082:1121	purified glycoprotein Support Protocol 1	1082:1121	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	3	from	cells/tissue	1210:1221	arg1	glycoproteins					1191:1203	glycoproteins	1191:1203	glycoproteins from cells/tissue	1191:1221	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	3	from	cells/tissue	1210:1221	arg1	Detection					1178:1186	Detection	1178:1186	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	4	4	theme	preparation	718:728	arg1	method					730:735	the detailed, streamlined sample preparation method	685:735	the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation	685:981	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	6	5	from	Detection	1178:1186	arg1	cells/tissue					1210:1221	cells/tissue	1210:1221	cells/tissue	1210:1221	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	1	6	theme	glycan	160:165	arg1	information					178:188	glycan structural information	160:188	glycan structural information	160:188	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	6	7	theme	Basic	1014:1018	arg1	Protocol					1020:1027	Basic Protocol 1	1014:1029	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	8	theme	Basic	1340:1344	arg1	Protocol					1346:1353	high-resolution nano-LC-MS/MS Basic Protocol 3	1310:1355	high-resolution nano-LC-MS/MS Basic Protocol 3	1310:1355	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	9	gly	glycoprotein	1091:1102	arg1	glycoprotein					1091:1102	purified glycoprotein Support Protocol 1	1082:1121	purified glycoprotein Support Protocol 1	1082:1121	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	10	theme	high-resolution	1310:1324	arg1	Protocol					1346:1353	high-resolution nano-LC-MS/MS Basic Protocol 3	1310:1355	high-resolution nano-LC-MS/MS Basic Protocol 3	1310:1355	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	4	11	theme	detailed	689:696	arg1	method					730:735	the detailed, streamlined sample preparation method	685:735	the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation	685:981	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	1	12	dep	along	149:153	arg1	with					155:158	with	155:158	with	155:158	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	1	13	gly	O-glycosylation	102:116	arg1	proteins					139:146	proteins	139:146	proteins	139:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	1	13	gly	O-glycosylation	102:116	arg1	sites					130:134	specific sites	121:134	specific sites of proteins	121:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	3	14	theme	key	603:605	arg1	glycoproteomics					538:552	Advanced tandem mass spectrometry-based glycoproteomics	498:552	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms	498:597	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	3	14	theme	key	603:605	arg1	elements					607:614	key elements	603:614	key elements for characterization of protein glycosylation	603:660	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	6	15	theme	glycoprotein	1091:1102	arg1	Protocol					1112:1119	purified glycoprotein Support Protocol 1	1082:1121	purified glycoprotein Support Protocol 1	1082:1121	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	3	16	theme	glycosylation	648:660	arg1	characterization					620:635	characterization	620:635	characterization of protein glycosylation	620:660	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	6	17	theme	site	1064:1067	arg1	occupancy					1069:1077	site occupancy	1064:1077	site occupancy	1064:1077	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	18	theme	glycopeptides	1395:1407	arg1	quantification					1377:1390	quantification	1377:1390	quantification	1377:1390	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	18	theme	glycopeptides	1395:1407	arg1	Identification					1358:1371	Identification	1358:1371	Identification	1358:1371	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	4	19	theme	bioinformatics-based	813:832	arg1	annotation					839:848	subsequent bioinformatics-based data annotation	802:848	subsequent bioinformatics-based data annotation	802:848	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	4	20	theme	chromatography-mass	748:766	arg1	spectrometry					768:779	liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation	741:848	spectrometry	768:779	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	4	21	gly	glycoprotein	956:967	arg1	glycoprotein					956:967	glycoprotein glycosylation	956:981	glycoprotein glycosylation	956:981	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	1	22	theme	proteins	139:146	arg1	proteins					139:146	proteins	139:146	proteins	139:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	1	22	theme	proteins	139:146	arg1	sites					130:134	specific sites	121:134	specific sites of proteins	121:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	6	23	theme	glycoproteins	1144:1156	arg1	digestion					1131:1139	In-gel digestion	1124:1139	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	4	24	theme	data	781:784	arg1	acquisition					786:796	data acquisition	781:796	data acquisition	781:796	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	4	25	theme	available	869:877	arg1	program					890:896	the publicly available GlycReSoft program	856:896	the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation	856:981	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	1	26	gly	glycoproteins	227:239	arg1	glycoproteins					227:239	glycoproteins	227:239	glycoproteins	227:239	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	4	27	dep	spectrometry	768:779	arg1	acquisition					786:796	data acquisition	781:796	data acquisition	781:796	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	6	28	dep	glycoproteins	1144:1156	arg1	Protocol					1166:1173	Support Protocol 2	1158:1175	glycoproteins Support Protocol 2	1144:1175	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	2	29	theme	specific	407:414	arg1	preparation					423:433	specific sample preparation	407:433	specific sample preparation	407:433	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
33761173	2	30	theme	data	459:462	arg1	analysis					464:471	data analysis	459:471	data analysis	459:471	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
33761173	2	31	theme	glycopeptides	392:404	arg1	behavior					380:387	the dissociation behavior	363:387	the dissociation behavior of glycopeptides	363:404	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
33761173	0	32	theme	Glycoproteomic	0:13	arg1	LC-MS					34:38	Glycoproteomic Sample Processing, LC-MS, and Data Analysis	0:57	LC-MS	34:38	Glycoproteomic Sample Processing, LC-MS, and Data Analysis Using GlycReSoft.					
33761173	1	33	theme	pathologic	260:269	arg1	functions					280:288	normal and pathologic cellular functions	249:288	normal and pathologic cellular functions	249:288	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	1	34	from	O-glycosylation	102:116	arg1	proteins					139:146	proteins	139:146	proteins	139:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	1	34	from	O-glycosylation	102:116	arg1	sites					130:134	specific sites	121:134	specific sites of proteins	121:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	2	35	theme	mass	436:439	arg1	spectrometry					441:452	mass spectrometry	436:452	mass spectrometry	436:452	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
33761173	0	36	theme	Processing	22:31	arg1	LC-MS					34:38	Glycoproteomic Sample Processing, LC-MS, and Data Analysis	0:57	LC-MS	34:38	Glycoproteomic Sample Processing, LC-MS, and Data Analysis Using GlycReSoft.					
33761173	5	37	dep	LLC	1009:1011	arg1	Periodicals					997:1007	Periodicals	997:1007	Periodicals	997:1007	© 2021 Wiley Periodicals LLC.					
33761173	2	38	theme	preparation	423:433	arg1	techniques					473:482	specific sample preparation, mass spectrometry, and data analysis techniques	407:482	specific sample preparation, mass spectrometry, and data analysis techniques	407:482	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
33761173	6	39	from	occupancy	1069:1077	arg1	Protocol					1112:1119	purified glycoprotein Support Protocol 1	1082:1121	purified glycoprotein Support Protocol 1	1082:1121	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	4	40	theme	glycoprotein	956:967	arg1	glycosylation					969:981	glycoprotein glycosylation	956:981	glycoprotein glycosylation	956:981	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	3	41	theme	tandem	507:512	arg1	glycoproteomics					538:552	Advanced tandem mass spectrometry-based glycoproteomics	498:552	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms	498:597	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	3	41	theme	tandem	507:512	arg1	elements					607:614	key elements	603:614	key elements for characterization of protein glycosylation	603:660	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	3	42	theme	spectrometry-based	519:536	arg1	glycoproteomics					538:552	Advanced tandem mass spectrometry-based glycoproteomics	498:552	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms	498:597	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	3	42	theme	spectrometry-based	519:536	arg1	elements					607:614	key elements	603:614	key elements for characterization of protein glycosylation	603:660	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	6	43	theme	enrichment	1244:1253	arg1	Protocol					1261:1268	glycopeptide enrichment Basic Protocol 2	1231:1270	glycopeptide enrichment Basic Protocol 2	1231:1270	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	1	44	theme	normal	249:254	arg1	functions					280:288	normal and pathologic cellular functions	249:288	normal and pathologic cellular functions	249:288	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	3	45	theme	powerful	567:574	arg1	data					576:579	powerful data	567:579	powerful data mining algorithms	567:597	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	4	46	theme	glycosylation	969:981	arg1	quantification					938:951	quantification	938:951	quantification	938:951	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	4	46	theme	glycosylation	969:981	arg1	identification					919:932	identification	919:932	identification	919:932	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	4	47	theme	sample	711:716	arg1	method					730:735	the detailed, streamlined sample preparation method	685:735	the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation	685:981	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	1	48	theme	structural	167:176	arg1	information					178:188	glycan structural information	160:188	glycan structural information	160:188	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	6	49	theme	nano-LC-MS/MS	1326:1338	arg1	Protocol					1346:1353	high-resolution nano-LC-MS/MS Basic Protocol 3	1310:1355	high-resolution nano-LC-MS/MS Basic Protocol 3	1310:1355	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	2	50	theme	such	299:302	arg1	glycosylation					304:316	such glycosylation	299:316	such glycosylation	299:316	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
33761173	6	51	theme	Support	1104:1110	arg1	Protocol					1112:1119	purified glycoprotein Support Protocol 1	1082:1121	purified glycoprotein Support Protocol 1	1082:1121	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	1	52	dep	roles	221:225	arg1	play					241:244	play	241:244	play in normal and pathologic cellular functions	241:288	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	3	53	theme	protein	640:646	arg1	glycosylation					648:660	protein glycosylation	640:660	protein glycosylation	640:660	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	6	54	theme	purified	1082:1089	arg1	Protocol					1112:1119	purified glycoprotein Support Protocol 1	1082:1121	purified glycoprotein Support Protocol 1	1082:1121	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	1	55	theme	O-glycosylation	102:116	arg1	Identification					77:90	Identification	77:90	Identification of N- and O-glycosylation on specific sites of proteins	77:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	6	56	theme	occupancy	1069:1077	arg1	Characterization					1032:1047	Characterization	1032:1047	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	1	57	theme	specific	121:128	arg1	proteins					139:146	proteins	139:146	proteins	139:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	1	57	theme	specific	121:128	arg1	sites					130:134	specific sites	121:134	specific sites of proteins	121:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	4	58	theme	subsequent	802:811	arg1	annotation					839:848	subsequent bioinformatics-based data annotation	802:848	subsequent bioinformatics-based data annotation	802:848	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	6	59	theme	Support	1158:1164	arg1	Protocol					1166:1173	Support Protocol 2	1158:1175	glycoproteins Support Protocol 2	1144:1175	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	4	60	theme	liquid	741:746	arg1	spectrometry					768:779	liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation	741:848	spectrometry	768:779	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	6	61	theme	In-gel	1124:1129	arg1	digestion					1131:1139	In-gel digestion	1124:1139	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	62	theme	glycopeptide	1231:1242	arg1	Protocol					1261:1268	glycopeptide enrichment Basic Protocol 2	1231:1270	glycopeptide enrichment Basic Protocol 2	1231:1270	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	63	gly	glycopeptides	1288:1300	arg2	glycopeptides					1288:1300	glycopeptides	1288:1300	glycopeptides	1288:1300	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	4	64	theme	GlycReSoft	879:888	arg1	program					890:896	the publicly available GlycReSoft program	856:896	the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation	856:981	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	0	65	theme	Sample	15:20	arg1	LC-MS					34:38	Glycoproteomic Sample Processing, LC-MS, and Data Analysis	0:57	LC-MS	34:38	Glycoproteomic Sample Processing, LC-MS, and Data Analysis Using GlycReSoft.					
33761173	1	66	from	sites	130:134	arg1	Identification					77:90	Identification	77:90	Identification of N- and O-glycosylation on specific sites of proteins	77:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	2	67	gly	glycopeptides	392:404	arg2	glycopeptides					392:404	glycopeptides	392:404	glycopeptides	392:404	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
33761173	4	68	theme	data	834:837	arg1	annotation					839:848	subsequent bioinformatics-based data annotation	802:848	subsequent bioinformatics-based data annotation	802:848	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	1	69	theme	cellular	271:278	arg1	functions					280:288	normal and pathologic cellular functions	249:288	normal and pathologic cellular functions	249:288	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	1	70	theme	N-	95:96	arg1	Identification					77:90	Identification	77:90	Identification of N- and O-glycosylation on specific sites of proteins	77:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	6	71	theme	glycoproteins	1191:1203	arg1	Detection					1178:1186	Detection	1178:1186	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	2	72	theme	spectrometry	441:452	arg1	techniques					473:482	specific sample preparation, mass spectrometry, and data analysis techniques	407:482	specific sample preparation, mass spectrometry, and data analysis techniques	407:482	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
33761173	6	73	gly	glycoproteins	1191:1203	arg1	glycoproteins					1191:1203	glycoproteins	1191:1203	glycoproteins from cells/tissue	1191:1221	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	74	from	Protocol	1112:1119	arg1	Characterization					1032:1047	Characterization	1032:1047	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	2	75	theme	sample	416:421	arg1	preparation					423:433	specific sample preparation	407:433	specific sample preparation	407:433	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
33761173	0	76	theme	Data	45:48	arg1	Analysis					50:57	Glycoproteomic Sample Processing, LC-MS, and Data Analysis	0:57	Analysis	50:57	Glycoproteomic Sample Processing, LC-MS, and Data Analysis Using GlycReSoft.					
33761173	6	77	from	Characterization	1032:1047	arg1	Protocol					1112:1119	purified glycoprotein Support Protocol 1	1082:1121	purified glycoprotein Support Protocol 1	1082:1121	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	4	78	dep	detailed	689:696	arg1	streamlined					699:709	streamlined	699:709	streamlined	699:709	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	6	79	theme	glycopeptides	1288:1300	arg1	Acquisition					1273:1283	Acquisition	1273:1283	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	2	80	theme	analysis	464:471	arg1	techniques					473:482	specific sample preparation, mass spectrometry, and data analysis techniques	407:482	specific sample preparation, mass spectrometry, and data analysis techniques	407:482	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
33761173	6	81	gly	glycopeptide	1231:1242	arg2	glycopeptide					1231:1242	glycopeptide enrichment Basic Protocol 2	1231:1270	glycopeptide enrichment Basic Protocol 2	1231:1270	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	3	82	theme	mass	514:517	arg1	glycoproteomics					538:552	Advanced tandem mass spectrometry-based glycoproteomics	498:552	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms	498:597	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	3	82	theme	mass	514:517	arg1	elements					607:614	key elements	603:614	key elements for characterization of protein glycosylation	603:660	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	1	83	from	N-	95:96	arg1	proteins					139:146	proteins	139:146	proteins	139:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	1	83	from	N-	95:96	arg1	sites					130:134	specific sites	121:134	specific sites of proteins	121:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	6	84	dep	Protocol	1020:1027	arg1	digestion					1131:1139	In-gel digestion	1124:1139	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	84	dep	Protocol	1020:1027	arg1	Acquisition					1273:1283	Acquisition	1273:1283	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	84	dep	Protocol	1020:1027	arg1	Detection					1178:1186	Detection	1178:1186	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	84	dep	Protocol	1020:1027	arg1	Identification					1358:1371	Identification	1358:1371	Identification	1358:1371	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	84	dep	Protocol	1020:1027	arg1	quantification					1377:1390	quantification	1377:1390	quantification	1377:1390	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	6	84	dep	Protocol	1020:1027	arg1	Characterization					1032:1047	Characterization	1032:1047	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.	1014:1425	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	3	85	theme	Advanced	498:505	arg1	glycoproteomics					538:552	Advanced tandem mass spectrometry-based glycoproteomics	498:552	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms	498:597	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	3	85	theme	Advanced	498:505	arg1	elements					607:614	key elements	603:614	key elements for characterization of protein glycosylation	603:660	Advanced tandem mass spectrometry-based glycoproteomics coupled with powerful data mining algorithms are key elements for characterization of protein glycosylation.					
33761173	1	86	from	Identification	77:90	arg1	proteins					139:146	proteins	139:146	proteins	139:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	1	86	from	Identification	77:90	arg1	sites					130:134	specific sites	121:134	specific sites of proteins	121:146	Identification of N- and O-glycosylation on specific sites of proteins, along with glycan structural information, is necessary to determine the roles glycoproteins play in normal and pathologic cellular functions.					
33761173	6	87	gly	glycopeptides	1395:1407	arg2	glycopeptides					1395:1407	glycopeptides	1395:1407	glycopeptides using GlycReSoft	1395:1424	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	4	88	theme	efficient	909:917	arg1	identification					919:932	identification	919:932	identification	919:932	This article includes the detailed, streamlined sample preparation method for liquid chromatography-mass spectrometry data acquisition and subsequent bioinformatics-based data annotation using the publicly available GlycReSoft program for highly efficient identification and quantification of glycoprotein glycosylation.					
33761173	6	89	gly	glycoproteins	1144:1156	arg1	glycoproteins					1144:1156	glycoproteins Support Protocol 2	1144:1175	glycoproteins Support Protocol 2	1144:1175	Basic Protocol 1: Characterization of glycans and site occupancy on purified glycoprotein Support Protocol 1: In-gel digestion of glycoproteins Support Protocol 2: Detection of glycoproteins from cells/tissue through glycopeptide enrichment Basic Protocol 2: Acquisition of glycopeptides through high-resolution nano-LC-MS/MS Basic Protocol 3: Identification and quantification of glycopeptides using GlycReSoft.					
33761173	2	90	theme	dissociation	367:378	arg1	behavior					380:387	the dissociation behavior	363:387	the dissociation behavior of glycopeptides	363:404	Because such glycosylation is macro- and micro-heterogeneous and alters the dissociation behavior of glycopeptides, specific sample preparation, mass spectrometry, and data analysis techniques are required.					
32286621	14	0	theme	CD	1978:1979	arg1	expression					1917:1926	expression	1917:1926	expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05)	1917:1994	GlcNAc and PM decreased expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05).					
32286621	14	1	dep	Ifng	1953:1956	arg1	%					1962:1962	-30%	1959:1962	-30%	1959:1962	GlcNAc and PM decreased expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05).					
32286621	14	1	dep	Ifng	1953:1956	arg1	%					1968:1968	-70%	1965:1968	-70%	1965:1968	GlcNAc and PM decreased expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05).					
32286621	11	2	theme	acetate	1471:1477	arg1	concentrations					1453:1466	Cecal concentrations	1447:1466	Cecal concentrations of acetate (+37%) and n-butyrate (+73%)	1447:1506	Cecal concentrations of acetate (+37%) and n-butyrate (+73%) were higher in 12-g/kg PM diet-fed rats versus CD (P < 0.05).					
32286621	13	3	theme	IgA+	1852:1855	arg1	cells					1864:1868	IgA+ plasma cells	1852:1868	IgA+ plasma cells (+25%) (all P < 0.05)	1852:1890	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	13	3	theme	IgA+	1852:1855	arg1	%					1874:1874	+25%	1871:1874	+25%	1871:1874	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	13	3	theme	IgA+	1852:1855	arg1	P < 0.05					1882:1889	all P < 0.05	1878:1889	all P < 0.05	1878:1889	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	5	4	theme	CD ± porcine	739:750	arg1	secretion					839:847	the daily mucin secretion	823:847	equivalent to 1.5 and 3 times the daily mucin secretion	793:847	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	4	theme	CD ± porcine	739:750	arg1	PM					767:768	PM	767:768	PM	767:768	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	4	theme	CD ± porcine	739:750	arg1	mucin					760:764	CD ± porcine stomach mucin	739:764	CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet	739:790	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	7	5	theme	cytokine	1192:1199	arg1	expression					1201:1210	inflammatory cytokine expression	1179:1210	inflammatory cytokine expression in the cecum	1179:1223	IgA+ plasma cells and regulatory T cells and inflammatory cytokine expression in the cecum were evaluated (experiment 4).					
32286621	12	6	theme	bacteria	1721:1728	arg1	numbers					1691:1697	increased numbers	1681:1697	increased numbers of butyrate-producing bacteria	1681:1728	Among O-glycan sugars, only GlcNAc produced higher n-butyrate concentrations (+68%) versus CD (P < 0.05), with increased numbers of butyrate-producing bacteria.					
32286621	15	7	dep	CONCLUSIONS	1997:2007	arg1	maintain					2061:2068	maintain	2061:2068	maintain mucosal immune homeostasis via large-bowel SCFA production in rats	2061:2135	CONCLUSIONS Mucin-derived O-glycans act as endogenous fiber and maintain mucosal immune homeostasis via large-bowel SCFA production in rats.					
32286621	15	7	dep	CONCLUSIONS	1997:2007	arg1	act					2033:2035	act	2033:2035	act as endogenous fiber	2033:2055	CONCLUSIONS Mucin-derived O-glycans act as endogenous fiber and maintain mucosal immune homeostasis via large-bowel SCFA production in rats.					
32286621	5	8	theme	male	679:682	arg1	rats					691:694	Normal male Wistar rats	672:694	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4)	672:728	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	9	theme	N-acetylneuraminic	921:938	arg1	acid					940:943	N-acetylneuraminic acid	921:943	N-acetylneuraminic acid	921:943	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	7	10	theme	T	1167:1167	arg1	cells					1169:1173	regulatory T cells	1156:1173	regulatory T cells	1156:1173	IgA+ plasma cells and regulatory T cells and inflammatory cytokine expression in the cecum were evaluated (experiment 4).					
32286621	6	11	theme	O-glycan	1102:1109	arg1	content					1111:1117	cecal O-glycan content	1096:1117	cecal O-glycan content	1096:1117	SCFAs, microbial composition, and cecal O-glycan content were assessed.					
32286621	3	12	link	mucin-derived	419:431	arg1	sugars					442:447	mucin-derived O-glycan sugars	419:447	mucin-derived O-glycan sugars	419:447	OBJECTIVE This study examined the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars and whether they affect gut immunity.					
32286621	15	13	theme	mucosal	2070:2076	arg1	homeostasis					2085:2095	mucosal immune homeostasis	2070:2095	mucosal immune homeostasis	2070:2095	CONCLUSIONS Mucin-derived O-glycans act as endogenous fiber and maintain mucosal immune homeostasis via large-bowel SCFA production in rats.					
32286621	12	14	theme	increased	1681:1689	arg1	numbers					1691:1697	increased numbers	1681:1697	increased numbers of butyrate-producing bacteria	1681:1728	Among O-glycan sugars, only GlcNAc produced higher n-butyrate concentrations (+68%) versus CD (P < 0.05), with increased numbers of butyrate-producing bacteria.					
32286621	10	15	theme	PM-fed	1395:1400	arg1	rats					1402:1405	PM-fed rats	1395:1405	PM-fed rats	1395:1405	PM-fed rats harbored more mucin-degrading bacteria.					
32286621	13	16	theme	cells	1807:1811	arg1	abundance					1752:1760	the abundance	1748:1760	the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%)	1748:1818	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	7	17	theme	plasma	1139:1144	arg1	cells					1146:1150	IgA+ plasma cells	1134:1150	IgA+ plasma cells	1134:1150	IgA+ plasma cells and regulatory T cells and inflammatory cytokine expression in the cecum were evaluated (experiment 4).					
32286621	14	18	theme	Ifng	1953:1956	arg1	expression					1917:1926	expression	1917:1926	expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05)	1917:1994	GlcNAc and PM decreased expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05).					
32286621	15	19	theme	endogenous	2040:2049	arg1	fiber					2051:2055	endogenous fiber	2040:2055	endogenous fiber	2040:2055	CONCLUSIONS Mucin-derived O-glycans act as endogenous fiber and maintain mucosal immune homeostasis via large-bowel SCFA production in rats.					
32286621	8	20	theme	RESULTS	1256:1262	arg1	secretion					1276:1284	RESULTS Daily mucin secretion	1256:1284	RESULTS Daily mucin secretion	1256:1284	RESULTS Daily mucin secretion corresponded to 43.2 μmol of O-glycans.					
32286621	15	21	from	production	2118:2127	arg1	rats					2132:2135	rats	2132:2135	rats	2132:2135	CONCLUSIONS Mucin-derived O-glycans act as endogenous fiber and maintain mucosal immune homeostasis via large-bowel SCFA production in rats.					
32286621	0	22	theme	Mucosal	60:66	arg1	Homeostasis					75:85	Mucosal Immune Homeostasis	60:85	Mucosal Immune Homeostasis	60:85	Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.					
32286621	15	23	theme	Mucin-derived	2009:2021	arg1	O-glycans					2023:2031	Mucin-derived O-glycans	2009:2031	Mucin-derived O-glycans	2009:2031	CONCLUSIONS Mucin-derived O-glycans act as endogenous fiber and maintain mucosal immune homeostasis via large-bowel SCFA production in rats.					
32286621	5	24	from	6	774:774	arg1	secretion					839:847	the daily mucin secretion	823:847	equivalent to 1.5 and 3 times the daily mucin secretion	793:847	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	24	from	6	774:774	arg1	PM					767:768	PM	767:768	PM	767:768	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	24	from	6	774:774	arg1	mucin					760:764	CD ± porcine stomach mucin	739:764	CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet	739:790	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	25	theme	equivalent	793:802	arg1	secretion					839:847	the daily mucin secretion	823:847	equivalent to 1.5 and 3 times the daily mucin secretion	793:847	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	25	theme	equivalent	793:802	arg1	mucin					760:764	CD ± porcine stomach mucin	739:764	CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet	739:790	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	4	26	theme	terminal	520:527	arg1	ileum					529:533	the terminal ileum	516:533	the terminal ileum	516:533	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	11	27	theme	diet-fed	1534:1541	arg1	rats					1543:1546	12-g/kg PM diet-fed rats	1523:1546	12-g/kg PM diet-fed rats	1523:1546	Cecal concentrations of acetate (+37%) and n-butyrate (+73%) were higher in 12-g/kg PM diet-fed rats versus CD (P < 0.05).					
32286621	13	28	theme	regulatory	1794:1803	arg1	cells					1807:1811	regulatory T cells	1794:1811	regulatory T cells (+33%)	1794:1818	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	13	28	theme	regulatory	1794:1803	arg1	%					1817:1817	+33%	1814:1817	+33%	1814:1817	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	11	29	theme	12-g/kg	1523:1529	arg1	rats					1543:1546	12-g/kg PM diet-fed rats	1523:1546	12-g/kg PM diet-fed rats	1523:1546	Cecal concentrations of acetate (+37%) and n-butyrate (+73%) were higher in 12-g/kg PM diet-fed rats versus CD (P < 0.05).					
32286621	0	30	theme	Short-Chain	91:101	arg1	Acid					109:112	Short-Chain Fatty Acid	91:112	Short-Chain Fatty Acid Production in Rat Cecum	91:136	Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.					
32286621	13	31	theme	cells	1777:1781	arg1	abundance					1752:1760	the abundance	1748:1760	the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%)	1748:1818	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	9	32	theme	Cecal	1326:1330	arg1	contents					1341:1348	Cecal O-glycan contents	1326:1348	Cecal O-glycan contents	1326:1348	Cecal O-glycan contents were comparable between CD- and PM-fed rats.					
32286621	4	33	theme	Mucin	495:499	arg1	secretion					501:509	METHODS Mucin secretion	487:509	METHODS Mucin secretion from the terminal ileum	487:533	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	5	34	theme	wk	703:704	arg1	wk					709:710	age 6 wk; 4 wk	697:710	age 6 wk; 4 wk for experiment 4	697:727	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	15	35	theme	large-bowel	2101:2111	arg1	production					2118:2127	large-bowel SCFA production	2101:2127	large-bowel SCFA production in rats	2101:2135	CONCLUSIONS Mucin-derived O-glycans act as endogenous fiber and maintain mucosal immune homeostasis via large-bowel SCFA production in rats.					
32286621	0	36	theme	Mucin-Derived	0:12	arg1	O-Glycans					14:22	Mucin-Derived O-Glycans	0:22	Mucin-Derived O-Glycans	0:22	Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.					
32286621	5	37	theme	15 g/kg	998:1004	arg1	diet					1006:1009	15 g/kg diet	998:1009	15 g/kg diet	998:1009	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	37	theme	15 g/kg	998:1004	arg1	d					968:968	14 d	965:968	14 d	965:968	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	38	theme	mucin	833:837	arg1	secretion					839:847	the daily mucin secretion	823:847	equivalent to 1.5 and 3 times the daily mucin secretion	793:847	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	38	theme	mucin	833:837	arg1	mucin					760:764	CD ± porcine stomach mucin	739:764	CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet	739:790	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	13	39	dep	increased	1738:1746	arg1	whereas					1831:1837	whereas	1831:1837	whereas	1831:1837	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	3	40	theme	fermentation	376:387	arg1	characteristics					389:403	the large-bowel fermentation characteristics	360:403	the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars	360:447	OBJECTIVE This study examined the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars and whether they affect gut immunity.					
32286621	4	41	theme	male	582:585	arg1	wk					606:607	age 6 wk	600:607	age 6 wk	600:607	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	4	41	theme	male	582:585	arg1	rats					594:597	ileorectostomized male Wistar rats	564:597	ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1)	564:669	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	9	42	theme	CD-	1374:1376	arg1	rats					1389:1392	CD- and PM-fed rats	1374:1392	CD- and PM-fed rats	1374:1392	Cecal O-glycan contents were comparable between CD- and PM-fed rats.					
32286621	15	43	link	Mucin-derived	2009:2021	arg1	O-glycans					2023:2031	Mucin-derived O-glycans	2009:2031	Mucin-derived O-glycans	2009:2031	CONCLUSIONS Mucin-derived O-glycans act as endogenous fiber and maintain mucosal immune homeostasis via large-bowel SCFA production in rats.					
32286621	3	44	theme	O-glycan	433:440	arg1	sugars					442:447	mucin-derived O-glycan sugars	419:447	mucin-derived O-glycan sugars	419:447	OBJECTIVE This study examined the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars and whether they affect gut immunity.					
32286621	4	45	theme	rats	594:597	arg1	feces					555:559	feces	555:559	feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1)	555:669	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	3	46	dep	OBJECTIVE	330:338	arg1	examined					351:358	examined	351:358	examined	351:358	OBJECTIVE This study examined the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars and whether they affect gut immunity.					
32286621	3	47	theme	gut	473:475	arg1	immunity					477:484	gut immunity	473:484	gut immunity	473:484	OBJECTIVE This study examined the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars and whether they affect gut immunity.					
32286621	0	48	theme	Endogenous	31:40	arg1	Fiber					42:46	Endogenous Fiber	31:46	Endogenous Fiber	31:46	Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.					
32286621	7	49	dep	evaluated	1230:1238	arg1	experiment					1241:1250	experiment 4	1241:1252	experiment 4	1241:1252	IgA+ plasma cells and regulatory T cells and inflammatory cytokine expression in the cecum were evaluated (experiment 4).					
32286621	12	50	theme	n-butyrate	1621:1630	arg1	%					1651:1651	+68%	1648:1651	+68%	1648:1651	Among O-glycan sugars, only GlcNAc produced higher n-butyrate concentrations (+68%) versus CD (P < 0.05), with increased numbers of butyrate-producing bacteria.					
32286621	12	50	theme	n-butyrate	1621:1630	arg1	concentrations					1632:1645	higher n-butyrate concentrations	1614:1645	higher n-butyrate concentrations (+68%)	1614:1652	Among O-glycan sugars, only GlcNAc produced higher n-butyrate concentrations (+68%) versus CD (P < 0.05), with increased numbers of butyrate-producing bacteria.					
32286621	9	51	theme	PM-fed	1382:1387	arg1	rats					1389:1392	CD- and PM-fed rats	1374:1392	CD- and PM-fed rats	1374:1392	Cecal O-glycan contents were comparable between CD- and PM-fed rats.					
32286621	12	52	theme	O-glycan	1576:1583	arg1	sugars					1585:1590	O-glycan sugars	1576:1590	O-glycan sugars	1576:1590	Among O-glycan sugars, only GlcNAc produced higher n-butyrate concentrations (+68%) versus CD (P < 0.05), with increased numbers of butyrate-producing bacteria.					
32286621	13	53	theme	plasma	1770:1775	arg1	cells					1777:1781	IgA+ plasma cells	1765:1781	IgA+ plasma cells (+29%)	1765:1788	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	13	53	theme	plasma	1770:1775	arg1	%					1787:1787	+29%	1784:1787	+29%	1784:1787	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	13	54	theme	plasma	1857:1862	arg1	cells					1864:1868	IgA+ plasma cells	1852:1868	IgA+ plasma cells (+25%) (all P < 0.05)	1852:1890	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	13	54	theme	plasma	1857:1862	arg1	%					1874:1874	+25%	1871:1874	+25%	1871:1874	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	13	54	theme	plasma	1857:1862	arg1	P < 0.05					1882:1889	all P < 0.05	1878:1889	all P < 0.05	1878:1889	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	12	55	theme	only	1593:1596	arg1	GlcNAc					1598:1603	only GlcNAc	1593:1603	only GlcNAc	1593:1603	Among O-glycan sugars, only GlcNAc produced higher n-butyrate concentrations (+68%) versus CD (P < 0.05), with increased numbers of butyrate-producing bacteria.					
32286621	1	56	theme	Intestinal	150:159	arg1	mucins					161:166	BACKGROUND Intestinal mucins	139:166	BACKGROUND Intestinal mucins	139:166	BACKGROUND Intestinal mucins escape digestion and enter the large bowel where they are degraded by the microbiota.					
32286621	4	57	theme	AIN76-based	617:627	arg1	CD					643:644	CD	643:644	CD	643:644	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	4	57	theme	AIN76-based	617:627	arg1	diet					637:640	an AIN76-based control diet	614:640	an AIN76-based control diet (CD) for 15 d (experiment 1)	614:669	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	4	57	theme	AIN76-based	617:627	arg1	experiment					657:666	experiment 1	657:668	experiment 1	657:668	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	11	58	theme	Cecal	1447:1451	arg1	concentrations					1453:1466	Cecal concentrations	1447:1466	Cecal concentrations of acetate (+37%) and n-butyrate (+73%)	1447:1506	Cecal concentrations of acetate (+37%) and n-butyrate (+73%) were higher in 12-g/kg PM diet-fed rats versus CD (P < 0.05).					
32286621	6	59	theme	microbial	1069:1077	arg1	composition					1079:1089	microbial composition	1069:1089	microbial composition	1069:1089	SCFAs, microbial composition, and cecal O-glycan content were assessed.					
32286621	8	60	theme	mucin	1270:1274	arg1	secretion					1276:1284	RESULTS Daily mucin secretion	1256:1284	RESULTS Daily mucin secretion	1256:1284	RESULTS Daily mucin secretion corresponded to 43.2 μmol of O-glycans.					
32286621	4	61	theme	age	600:602	arg1	wk					606:607	age 6 wk	600:607	age 6 wk	600:607	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	4	61	theme	age	600:602	arg1	rats					594:597	ileorectostomized male Wistar rats	564:597	ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1)	564:669	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	4	62	from	ileum	529:533	arg1	secretion					501:509	METHODS Mucin secretion	487:509	METHODS Mucin secretion from the terminal ileum	487:533	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	5	63	theme	10 g/kg	1023:1029	arg1	diet					1031:1034	10 g/kg diet	1023:1034	10 g/kg diet	1023:1034	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	63	theme	10 g/kg	1023:1029	arg1	d					968:968	14 d	965:968	14 d	965:968	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	64	theme	12 g/kg	779:785	arg1	diet					787:790	12 g/kg diet	779:790	12 g/kg diet	779:790	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	14	65	dep	Tnfa	1931:1934	arg1	%					1940:1940	-30%	1937:1940	-30%	1937:1940	GlcNAc and PM decreased expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05).					
32286621	14	65	dep	Tnfa	1931:1934	arg1	%					1946:1946	-40%	1943:1946	-40%	1943:1946	GlcNAc and PM decreased expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05).					
32286621	0	66	theme	Rat	128:130	arg1	Cecum					132:136	Rat Cecum	128:136	Rat Cecum	128:136	Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.					
32286621	2	67	theme	large-bowel	291:301	arg1	physiology					303:312	large-bowel physiology	291:312	large-bowel physiology	291:312	To what extent and how mucins impact large-bowel physiology remain unclear.					
32286621	1	68	theme	large	199:203	arg1	bowel					205:209	the large bowel	195:209	the large bowel	195:209	BACKGROUND Intestinal mucins escape digestion and enter the large bowel where they are degraded by the microbiota.					
32286621	12	69	theme	butyrate-producing	1702:1719	arg1	bacteria					1721:1728	butyrate-producing bacteria	1702:1728	butyrate-producing bacteria	1702:1728	Among O-glycan sugars, only GlcNAc produced higher n-butyrate concentrations (+68%) versus CD (P < 0.05), with increased numbers of butyrate-producing bacteria.					
32286621	7	70	theme	inflammatory	1179:1190	arg1	expression					1201:1210	inflammatory cytokine expression	1179:1210	inflammatory cytokine expression in the cecum	1179:1223	IgA+ plasma cells and regulatory T cells and inflammatory cytokine expression in the cecum were evaluated (experiment 4).					
32286621	5	71	theme	Wistar	684:689	arg1	rats					691:694	Normal male Wistar rats	672:694	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4)	672:728	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	4	72	theme	METHODS	487:493	arg1	secretion					501:509	METHODS Mucin secretion	487:509	METHODS Mucin secretion from the terminal ileum	487:533	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	6	73	theme	cecal	1096:1100	arg1	content					1111:1117	cecal O-glycan content	1096:1117	cecal O-glycan content	1096:1117	SCFAs, microbial composition, and cecal O-glycan content were assessed.					
32286621	5	74	theme	10 g/kg	948:954	arg1	diet					956:959	10 g/kg diet	948:959	10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4)	948:1059	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	15	75	theme	immune	2078:2083	arg1	homeostasis					2085:2095	mucosal immune homeostasis	2070:2095	mucosal immune homeostasis	2070:2095	CONCLUSIONS Mucin-derived O-glycans act as endogenous fiber and maintain mucosal immune homeostasis via large-bowel SCFA production in rats.					
32286621	5	76	theme	Normal	672:677	arg1	rats					691:694	Normal male Wistar rats	672:694	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4)	672:728	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	0	77	from	Production	114:123	arg1	Cecum					132:136	Rat Cecum	128:136	Rat Cecum	128:136	Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.					
32286621	7	78	theme	regulatory	1156:1165	arg1	cells					1169:1173	regulatory T cells	1156:1173	regulatory T cells	1156:1173	IgA+ plasma cells and regulatory T cells and inflammatory cytokine expression in the cecum were evaluated (experiment 4).					
32286621	8	79	theme	Daily	1264:1268	arg1	secretion					1276:1284	RESULTS Daily mucin secretion	1256:1284	RESULTS Daily mucin secretion	1256:1284	RESULTS Daily mucin secretion corresponded to 43.2 μmol of O-glycans.					
32286621	0	80	theme	Immune	68:73	arg1	Homeostasis					75:85	Mucosal Immune Homeostasis	60:85	Mucosal Immune Homeostasis	60:85	Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.					
32286621	5	81	from	diet	787:790	arg1	secretion					839:847	the daily mucin secretion	823:847	equivalent to 1.5 and 3 times the daily mucin secretion	793:847	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	81	from	diet	787:790	arg1	PM					767:768	PM	767:768	PM	767:768	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	81	from	diet	787:790	arg1	mucin					760:764	CD ± porcine stomach mucin	739:764	CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet	739:790	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	1	82	theme	BACKGROUND	139:148	arg1	mucins					161:166	BACKGROUND Intestinal mucins	139:166	BACKGROUND Intestinal mucins	139:166	BACKGROUND Intestinal mucins escape digestion and enter the large bowel where they are degraded by the microbiota.					
32286621	7	83	from	expression	1201:1210	arg1	cecum					1219:1223	the cecum	1215:1223	the cecum	1215:1223	IgA+ plasma cells and regulatory T cells and inflammatory cytokine expression in the cecum were evaluated (experiment 4).					
32286621	5	84	dep	rats	691:694	arg1	wk					709:710	age 6 wk; 4 wk	697:710	age 6 wk; 4 wk for experiment 4	697:727	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	7	85	theme	IgA+	1134:1137	arg1	cells					1146:1150	IgA+ plasma cells	1134:1150	IgA+ plasma cells	1134:1150	IgA+ plasma cells and regulatory T cells and inflammatory cytokine expression in the cecum were evaluated (experiment 4).					
32286621	2	86	dep	extent	262:267	arg1	what					257:260	what	257:260	what	257:260	To what extent and how mucins impact large-bowel physiology remain unclear.					
32286621	5	87	theme	age	697:699	arg1	wk					709:710	age 6 wk; 4 wk	697:710	age 6 wk; 4 wk for experiment 4	697:727	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	0	88	theme	Fatty	103:107	arg1	Acid					109:112	Short-Chain Fatty Acid	91:112	Short-Chain Fatty Acid Production in Rat Cecum	91:136	Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.					
32286621	13	89	theme	T	1805:1805	arg1	cells					1807:1811	regulatory T cells	1794:1811	regulatory T cells (+33%)	1794:1818	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	13	89	theme	T	1805:1805	arg1	%					1817:1817	+33%	1814:1817	+33%	1814:1817	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	3	90	theme	large-bowel	364:374	arg1	characteristics					389:403	the large-bowel fermentation characteristics	360:403	the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars	360:447	OBJECTIVE This study examined the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars and whether they affect gut immunity.					
32286621	11	91	theme	PM	1531:1532	arg1	rats					1543:1546	12-g/kg PM diet-fed rats	1523:1546	12-g/kg PM diet-fed rats	1523:1546	Cecal concentrations of acetate (+37%) and n-butyrate (+73%) were higher in 12-g/kg PM diet-fed rats versus CD (P < 0.05).					
32286621	9	92	theme	O-glycan	1332:1339	arg1	contents					1341:1348	Cecal O-glycan contents	1326:1348	Cecal O-glycan contents	1326:1348	Cecal O-glycan contents were comparable between CD- and PM-fed rats.					
32286621	8	93	theme	O-glycans	1315:1323	arg1	μmol					1307:1310	43.2 μmol	1302:1310	43.2 μmol of O-glycans	1302:1323	RESULTS Daily mucin secretion corresponded to 43.2 μmol of O-glycans.					
32286621	14	94	theme	Tnfa	1931:1934	arg1	expression					1917:1926	expression	1917:1926	expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05)	1917:1994	GlcNAc and PM decreased expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05).					
32286621	15	95	theme	SCFA	2113:2116	arg1	production					2118:2127	large-bowel SCFA production	2101:2127	large-bowel SCFA production in rats	2101:2135	CONCLUSIONS Mucin-derived O-glycans act as endogenous fiber and maintain mucosal immune homeostasis via large-bowel SCFA production in rats.					
32286621	5	96	theme	daily	827:831	arg1	secretion					839:847	the daily mucin secretion	823:847	equivalent to 1.5 and 3 times the daily mucin secretion	793:847	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	96	theme	daily	827:831	arg1	mucin					760:764	CD ± porcine stomach mucin	739:764	CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet	739:790	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	7	97	from	cells	1146:1150	arg1	cecum					1219:1223	the cecum	1215:1223	the cecum	1215:1223	IgA+ plasma cells and regulatory T cells and inflammatory cytokine expression in the cecum were evaluated (experiment 4).					
32286621	3	98	theme	mucins	408:413	arg1	characteristics					389:403	the large-bowel fermentation characteristics	360:403	the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars	360:447	OBJECTIVE This study examined the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars and whether they affect gut immunity.					
32286621	4	99	theme	Wistar	587:592	arg1	wk					606:607	age 6 wk	600:607	age 6 wk	600:607	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	4	99	theme	Wistar	587:592	arg1	rats					594:597	ileorectostomized male Wistar rats	564:597	ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1)	564:669	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	14	100	dep	CD	1978:1979	arg1	P < 0.05					1986:1993	P < 0.05	1986:1993	P < 0.05	1986:1993	GlcNAc and PM decreased expression of Tnfa (-30%, -40%) and Ifng (-30%, -70%) versus CD (all P < 0.05).					
32286621	10	101	theme	mucin-degrading	1421:1435	arg1	bacteria					1437:1444	more mucin-degrading bacteria	1416:1444	more mucin-degrading bacteria	1416:1444	PM-fed rats harbored more mucin-degrading bacteria.					
32286621	5	102	theme	stomach	752:758	arg1	secretion					839:847	the daily mucin secretion	823:847	equivalent to 1.5 and 3 times the daily mucin secretion	793:847	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	102	theme	stomach	752:758	arg1	PM					767:768	PM	767:768	PM	767:768	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	5	102	theme	stomach	752:758	arg1	mucin					760:764	CD ± porcine stomach mucin	739:764	CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet	739:790	Normal male Wistar rats (age 6 wk; 4 wk for experiment 4) were fed CD ± porcine stomach mucin (PM) at 6 or 12 g/kg diet, equivalent to 1.5 and 3 times the daily mucin secretion, for 14 d (experiment 2); CD ± N-acetylglucosamine (GlcNAc), fucose, or N-acetylneuraminic acid at 10 g/kg diet for 14 d (experiment 3); or CD ± PM (15 g/kg diet) or GlcNAc (10 g/kg diet) for 29 d (experiment 4).					
32286621	12	103	theme	higher	1614:1619	arg1	%					1651:1651	+68%	1648:1651	+68%	1648:1651	Among O-glycan sugars, only GlcNAc produced higher n-butyrate concentrations (+68%) versus CD (P < 0.05), with increased numbers of butyrate-producing bacteria.					
32286621	12	103	theme	higher	1614:1619	arg1	concentrations					1632:1645	higher n-butyrate concentrations	1614:1645	higher n-butyrate concentrations (+68%)	1614:1652	Among O-glycan sugars, only GlcNAc produced higher n-butyrate concentrations (+68%) versus CD (P < 0.05), with increased numbers of butyrate-producing bacteria.					
32286621	7	104	from	cells	1169:1173	arg1	cecum					1219:1223	the cecum	1215:1223	the cecum	1215:1223	IgA+ plasma cells and regulatory T cells and inflammatory cytokine expression in the cecum were evaluated (experiment 4).					
32286621	3	105	theme	sugars	442:447	arg1	characteristics					389:403	the large-bowel fermentation characteristics	360:403	the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars	360:447	OBJECTIVE This study examined the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars and whether they affect gut immunity.					
32286621	0	106	theme	Acid	109:112	arg1	Production					114:123	Short-Chain Fatty Acid Production	91:123	Short-Chain Fatty Acid Production in Rat Cecum	91:136	Mucin-Derived O-Glycans Act as Endogenous Fiber and Sustain Mucosal Immune Homeostasis via Short-Chain Fatty Acid Production in Rat Cecum.					
32286621	3	107	theme	mucin-derived	419:431	arg1	sugars					442:447	mucin-derived O-glycan sugars	419:447	mucin-derived O-glycan sugars	419:447	OBJECTIVE This study examined the large-bowel fermentation characteristics of mucins and mucin-derived O-glycan sugars and whether they affect gut immunity.					
32286621	4	108	theme	ileorectostomized	564:580	arg1	wk					606:607	age 6 wk	600:607	age 6 wk	600:607	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	4	108	theme	ileorectostomized	564:580	arg1	rats					594:597	ileorectostomized male Wistar rats	564:597	ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1)	564:669	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	13	109	theme	IgA+	1765:1768	arg1	cells					1777:1781	IgA+ plasma cells	1765:1781	IgA+ plasma cells (+29%)	1765:1788	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	13	109	theme	IgA+	1765:1768	arg1	%					1787:1787	+29%	1784:1787	+29%	1784:1787	GlcNAc increased the abundance of IgA+ plasma cells (+29%) and regulatory T cells (+33%) versus CD, whereas PM increased IgA+ plasma cells (+25%) (all P < 0.05).					
32286621	11	110	theme	n-butyrate	1490:1499	arg1	concentrations					1453:1466	Cecal concentrations	1447:1466	Cecal concentrations of acetate (+37%) and n-butyrate (+73%)	1447:1506	Cecal concentrations of acetate (+37%) and n-butyrate (+73%) were higher in 12-g/kg PM diet-fed rats versus CD (P < 0.05).					
32286621	4	111	theme	control	629:635	arg1	CD					643:644	CD	643:644	CD	643:644	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	4	111	theme	control	629:635	arg1	diet					637:640	an AIN76-based control diet	614:640	an AIN76-based control diet (CD) for 15 d (experiment 1)	614:669	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32286621	4	111	theme	control	629:635	arg1	experiment					657:666	experiment 1	657:668	experiment 1	657:668	METHODS Mucin secretion from the terminal ileum was determined from feces of ileorectostomized male Wistar rats (age 6 wk) fed an AIN76-based control diet (CD) for 15 d (experiment 1).					
32423029	3	0	theme	consensus	873:881	arg1	sequence					883:890	the same consensus sequence	864:890	the same consensus sequence	864:890	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	5	1	theme	O-glucose	1103:1111	arg1	elongation					1089:1098	the xylosyl elongation	1077:1098	the xylosyl elongation of O-glucose	1077:1111	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	5	2	from	expression	1144:1153	arg1	surface					1194:1200	the surface	1190:1200	the surface of HEK293T cells	1190:1217	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	3	3	theme	mass	654:657	arg1	spectrometer					659:670	an Orbitrap Fusion mass spectrometer	635:670	an Orbitrap Fusion mass spectrometer	635:670	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	4	4	theme	attachment	914:923	arg1	levels					898:903	High levels	893:903	High levels of O-Glc attachment and xylosyl elongation	893:946	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	6	5	theme	reduced	1403:1409	arg1	secretion					1411:1419	a reduced secretion	1401:1419	a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells	1401:1497	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	6	6	theme	knockout	1484:1491	arg1	cells					1493:1497	GXYLT1 and GXYLT2 double knockout cells	1459:1497	GXYLT1 and GXYLT2 double knockout cells	1459:1497	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	2	7	theme	growth	515:520	arg1	repeats					540:546	29-36 epidermal growth factor-like (EGF) repeats	499:546	29-36 epidermal growth factor-like (EGF) repeats	499:546	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	0	8	theme	Surface	109:115	arg1	Trafficking					117:127	Notch Cell Surface Trafficking	98:127	Notch Cell Surface Trafficking	98:127	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	6	9	theme	GXYLT2	1470:1475	arg1	cells					1493:1497	GXYLT1 and GXYLT2 double knockout cells	1459:1497	GXYLT1 and GXYLT2 double knockout cells	1459:1497	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	6	10	theme	significant	1547:1557	arg1	role					1559:1562	a significant role	1545:1562	a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors	1545:1657	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	6	11	theme	GXYLT1	1459:1464	arg1	cells					1493:1497	GXYLT1 and GXYLT2 double knockout cells	1459:1497	GXYLT1 and GXYLT2 double knockout cells	1459:1497	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	3	12	theme	C1-X-S-X-	800:808	arg1	sequence					790:797	the O-Glc consensus sequence	770:797	the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2)	770:817	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	12	theme	C1-X-S-X-	800:808	arg1	-C2					814:816	C1-X-S-X-(P/A)-C2	800:816	C1-X-S-X-(P/A)-C2	800:816	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	5	13	theme	NOTCH2	1180:1185	arg1	expression					1144:1153	the expression	1140:1153	the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells	1140:1217	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	1	14	theme	Notch	373:377	arg1	function					388:395	the Notch receptor function	369:395	the Notch receptor function	369:395	Biochemical and genetic studies have indicated that O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc) is critical for Notch signaling; however, it is not fully understood how O-glycans regulate the Notch receptor function.					
32423029	6	15	theme	ECDs	1451:1454	arg1	secretion					1411:1419	a reduced secretion	1401:1419	a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells	1401:1497	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	1	16	theme	Biochemical	130:140	arg1	studies					154:160	Biochemical and genetic studies	130:160	Biochemical and genetic studies	130:160	Biochemical and genetic studies have indicated that O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc) is critical for Notch signaling; however, it is not fully understood how O-glycans regulate the Notch receptor function.					
32423029	3	17	theme	EGF	743:745	arg1	repeats					747:753	all 17 EGF repeats	736:753	all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2)	736:817	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	5	18	theme	glucoside	1011:1019	arg1	xylosyltransferases					1021:1039	both glucoside xylosyltransferases	1006:1039	both glucoside xylosyltransferases	1006:1039	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	5	18	theme	glucoside	1011:1019	arg1	GXYLT2					1053:1058	GXYLT2	1053:1058	GXYLT2	1053:1058	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	5	18	theme	glucoside	1011:1019	arg1	GXYLT1					1042:1047	GXYLT1	1042:1047	GXYLT1	1042:1047	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	2	19	with	proteins	439:446	arg1	ECD					482:484	ECD	482:484	ECD	482:484	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	2	19	with	proteins	439:446	arg1	domains					473:479	large extracellular domains	453:479	large extracellular domains (ECD)	453:485	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	1	20	theme	genetic	146:152	arg1	studies					154:160	Biochemical and genetic studies	130:160	Biochemical and genetic studies	130:160	Biochemical and genetic studies have indicated that O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc) is critical for Notch signaling; however, it is not fully understood how O-glycans regulate the Notch receptor function.					
32423029	2	21	theme	large	453:457	arg1	ECD					482:484	ECD	482:484	ECD	482:484	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	2	21	theme	large	453:457	arg1	domains					473:479	large extracellular domains	453:479	large extracellular domains (ECD)	453:485	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	6	22	theme	Notch	1606:1610	arg1	ECDs					1612:1615	the Notch ECDs	1602:1615	the Notch ECDs in the quality control of Notch receptors	1602:1657	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	3	23	theme	O-Glc	774:778	arg1	sequence					790:797	the O-Glc consensus sequence	770:797	the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2)	770:817	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	23	theme	O-Glc	774:778	arg1	-C2					814:816	C1-X-S-X-(P/A)-C2	800:816	C1-X-S-X-(P/A)-C2	800:816	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	0	24	theme	NOTCH1	70:75	arg1	NOTCH1					70:75	NOTCH1	70:75	NOTCH1	70:75	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	0	24	theme	NOTCH1	70:75	arg1	Domain					60:65	the Extracellular Domain	42:65	the Extracellular Domain of NOTCH1 and NOTCH2	42:86	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	0	24	theme	NOTCH1	70:75	arg1	NOTCH2					81:86	NOTCH2	81:86	NOTCH2	81:86	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	3	25	with	stoichiometries	717:731	arg1	sequence					883:890	the same consensus sequence	864:890	the same consensus sequence	864:890	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	26	theme	P/A	810:812	arg1	sequence					790:797	the O-Glc consensus sequence	770:797	the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2)	770:817	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	26	theme	P/A	810:812	arg1	-C2					814:816	C1-X-S-X-(P/A)-C2	800:816	C1-X-S-X-(P/A)-C2	800:816	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	5	27	theme	NOTCH1	1169:1174	arg1	expression					1144:1153	the expression	1140:1153	the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells	1140:1217	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	0	28	theme	NOTCH2	81:86	arg1	NOTCH1					70:75	NOTCH1	70:75	NOTCH1	70:75	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	0	28	theme	NOTCH2	81:86	arg1	Domain					60:65	the Extracellular Domain	42:65	the Extracellular Domain of NOTCH1 and NOTCH2	42:86	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	0	28	theme	NOTCH2	81:86	arg1	NOTCH2					81:86	NOTCH2	81:86	NOTCH2	81:86	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	6	29	from	secretion	1411:1419	arg1	cells					1493:1497	GXYLT1 and GXYLT2 double knockout cells	1459:1497	GXYLT1 and GXYLT2 double knockout cells	1459:1497	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	6	30	theme	O-Glc	1585:1589	arg1	glycans					1591:1597	O-Glc glycans	1585:1597	O-Glc glycans	1585:1597	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	1	31	link	O-linked	182:189	arg1	glycosylation					191:203	O-linked glycosylation	182:203	O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc)	182:275	Biochemical and genetic studies have indicated that O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc) is critical for Notch signaling; however, it is not fully understood how O-glycans regulate the Notch receptor function.					
32423029	0	32	theme	Notch	98:102	arg1	Trafficking					117:127	Notch Cell Surface Trafficking	98:127	Notch Cell Surface Trafficking	98:127	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	6	33	from	role	1559:1562	arg1	ECDs					1612:1615	the Notch ECDs	1602:1615	the Notch ECDs in the quality control of Notch receptors	1602:1657	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	6	34	theme	elongation	1571:1580	arg1	role					1559:1562	a significant role	1545:1562	a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors	1545:1657	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	3	35	from	glycans	573:579	arg1	NOTCH2					595:600	NOTCH2	595:600	NOTCH2	595:600	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	35	from	glycans	573:579	arg1	NOTCH1					584:589	NOTCH1	584:589	NOTCH1	584:589	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	0	36	theme	Xylosyl	0:6	arg1	Extension					8:16	Xylosyl Extension	0:16	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2	0:86	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	3	37	with	structures	702:711	arg1	sequence					883:890	the same consensus sequence	864:890	the same consensus sequence	864:890	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	38	theme	EGF	837:839	arg1	repeats					841:847	16 out of 17 EGF repeats	824:847	16 out of 17 EGF repeats of NOTCH2	824:857	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	4	39	theme	elongation	937:946	arg1	levels					898:903	High levels	893:903	High levels of O-Glc attachment and xylosyl elongation	893:946	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	6	40	theme	receptors	1649:1657	arg1	control					1632:1638	the quality control	1620:1638	the quality control of Notch receptors	1620:1657	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	5	41	theme	xylosyl	1081:1087	arg1	elongation					1089:1098	the xylosyl elongation	1077:1098	the xylosyl elongation of O-glucose	1077:1111	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	5	42	theme	cells	1213:1217	arg1	surface					1194:1200	the surface	1190:1200	the surface of HEK293T cells	1190:1217	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	0	43	theme	Glycans	31:37	arg1	Extension					8:16	Xylosyl Extension	0:16	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2	0:86	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	4	44	theme	High	893:896	arg1	levels					898:903	High levels	893:903	High levels of O-Glc attachment and xylosyl elongation	893:946	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	4	45	theme	O-Glc	908:912	arg1	attachment					914:923	O-Glc attachment	908:923	O-Glc attachment	908:923	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	6	46	theme	quality	1624:1630	arg1	control					1632:1638	the quality control	1620:1638	the quality control of Notch receptors	1620:1657	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	1	47	theme	Notch	293:297	arg1	signaling					299:307	Notch signaling	293:307	Notch signaling	293:307	Biochemical and genetic studies have indicated that O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc) is critical for Notch signaling; however, it is not fully understood how O-glycans regulate the Notch receptor function.					
32423029	5	48	theme	NOTCH1	1284:1289	arg1	expression					1256:1265	the cell surface expression	1239:1265	the cell surface expression of overexpressed NOTCH1 and NOTCH2	1239:1300	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	6	49	theme	secretion	1364:1372	arg1	assays					1374:1379	In vitro secretion assays	1355:1379	In vitro secretion assays	1355:1379	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	4	50	theme	EGF	988:990	arg1	repeats					992:998	most NOTCH1 and NOTCH2 EGF repeats	965:998	most NOTCH1 and NOTCH2 EGF repeats	965:998	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	6	51	theme	In	1355:1356	arg1	assays					1374:1379	In vitro secretion assays	1355:1379	In vitro secretion assays	1355:1379	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	2	52	theme	type-I	418:423	arg1	receptors					404:412	Notch receptors	398:412	Notch receptors	398:412	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	2	52	theme	type-I	418:423	arg1	proteins					439:446	type-I transmembrane proteins	418:446	type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats	418:546	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	3	53	theme	same	868:871	arg1	sequence					883:890	the same consensus sequence	864:890	the same consensus sequence	864:890	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	54	theme	Fusion	647:652	arg1	spectrometer					659:670	an Orbitrap Fusion mass spectrometer	635:670	an Orbitrap Fusion mass spectrometer	635:670	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	5	55	theme	surface	1248:1254	arg1	expression					1256:1265	the cell surface expression	1239:1265	the cell surface expression of overexpressed NOTCH1 and NOTCH2	1239:1300	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	4	56	theme	most	965:968	arg1	repeats					992:998	most NOTCH1 and NOTCH2 EGF repeats	965:998	most NOTCH1 and NOTCH2 EGF repeats	965:998	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	1	57	gly	glycosylation	191:203	arg1	Notch					293:297	Notch signaling	293:307	Notch signaling	293:307	Biochemical and genetic studies have indicated that O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc) is critical for Notch signaling; however, it is not fully understood how O-glycans regulate the Notch receptor function.					
32423029	6	58	theme	NOTCH1	1433:1438	arg1	ECDs					1451:1454	both the NOTCH1 and NOTCH2 ECDs	1424:1454	ECDs	1451:1454	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	4	59	located	detected	953:960	arg1	repeats					992:998	most NOTCH1 and NOTCH2 EGF repeats	965:998	most NOTCH1 and NOTCH2 EGF repeats	965:998	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	4	59	located	detected	953:960	arg2	levels					898:903	High levels	893:903	High levels of O-Glc attachment and xylosyl elongation	893:946	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	5	60	theme	NOTCH2	1295:1300	arg1	expression					1256:1265	the cell surface expression	1239:1265	the cell surface expression of overexpressed NOTCH1 and NOTCH2	1239:1300	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	1	61	theme	O-linked	182:189	arg1	glycosylation					191:203	O-linked glycosylation	182:203	O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc)	182:275	Biochemical and genetic studies have indicated that O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc) is critical for Notch signaling; however, it is not fully understood how O-glycans regulate the Notch receptor function.					
32423029	6	62	theme	type	1522:1525	arg1	cells					1527:1531	the wild type cells	1513:1531	the wild type cells	1513:1531	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	2	63	theme	epidermal	505:513	arg1	repeats					540:546	29-36 epidermal growth factor-like (EGF) repeats	499:546	29-36 epidermal growth factor-like (EGF) repeats	499:546	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	2	64	theme	EGF	535:537	arg1	repeats					540:546	29-36 epidermal growth factor-like (EGF) repeats	499:546	29-36 epidermal growth factor-like (EGF) repeats	499:546	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	3	65	theme	O-Glc	567:571	arg1	glycans					573:579	O-Glc glycans	567:579	O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer	567:670	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	2	66	theme	factor-like	522:532	arg1	repeats					540:546	29-36 epidermal growth factor-like (EGF) repeats	499:546	29-36 epidermal growth factor-like (EGF) repeats	499:546	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	3	67	theme	NOTCH1	758:763	arg1	repeats					747:753	all 17 EGF repeats	736:753	all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2)	736:817	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	67	theme	NOTCH1	758:763	arg1	repeats					841:847	16 out of 17 EGF repeats	824:847	16 out of 17 EGF repeats of NOTCH2	824:857	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	1	68	theme	receptor	379:386	arg1	function					388:395	the Notch receptor function	369:395	the Notch receptor function	369:395	Biochemical and genetic studies have indicated that O-linked glycosylation such as O-glucose (Glc), fucose (Fuc), and N-acetylglucosamine (GlcNAc) is critical for Notch signaling; however, it is not fully understood how O-glycans regulate the Notch receptor function.					
32423029	6	69	theme	NOTCH2	1444:1449	arg1	ECDs					1451:1454	both the NOTCH1 and NOTCH2 ECDs	1424:1454	ECDs	1451:1454	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	2	70	theme	extracellular	459:471	arg1	ECD					482:484	ECD	482:484	ECD	482:484	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	2	70	theme	extracellular	459:471	arg1	domains					473:479	large extracellular domains	453:479	large extracellular domains (ECD)	453:485	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	3	71	theme	repeats	747:753	arg1	structures					702:711	the structures	698:711	the structures	698:711	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	71	theme	repeats	747:753	arg1	stoichiometries					717:731	stoichiometries	717:731	stoichiometries	717:731	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	72	with	NOTCH1	758:763	arg1	sequence					790:797	the O-Glc consensus sequence	770:797	the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2)	770:817	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	72	with	NOTCH1	758:763	arg1	-C2					814:816	C1-X-S-X-(P/A)-C2	800:816	C1-X-S-X-(P/A)-C2	800:816	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	0	73	from	Extension	8:16	arg1	NOTCH1					70:75	NOTCH1	70:75	NOTCH1	70:75	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	0	73	from	Extension	8:16	arg1	Domain					60:65	the Extracellular Domain	42:65	the Extracellular Domain of NOTCH1 and NOTCH2	42:86	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	0	73	from	Extension	8:16	arg1	NOTCH2					81:86	NOTCH2	81:86	NOTCH2	81:86	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	5	74	theme	endogenous	1158:1167	arg1	NOTCH1					1169:1174	endogenous NOTCH1	1158:1174	endogenous NOTCH1	1158:1174	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	3	75	theme	consensus	780:788	arg1	sequence					790:797	the O-Glc consensus sequence	770:797	the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2)	770:817	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	75	theme	consensus	780:788	arg1	-C2					814:816	C1-X-S-X-(P/A)-C2	800:816	C1-X-S-X-(P/A)-C2	800:816	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	6	76	theme	wild	1517:1520	arg1	cells					1527:1531	the wild type cells	1513:1531	the wild type cells	1513:1531	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	6	77	theme	glycans	1591:1597	arg1	elongation					1571:1580	the elongation	1567:1580	the elongation of O-Glc glycans	1567:1597	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	2	78	contain	containing	488:497	arg2	repeats					540:546	29-36 epidermal growth factor-like (EGF) repeats	499:546	29-36 epidermal growth factor-like (EGF) repeats	499:546	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	2	78	contain	containing	488:497	arg1	domains					473:479	large extracellular domains	453:479	large extracellular domains (ECD)	453:485	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	2	78	contain	containing	488:497	arg1	ECD					482:484	ECD	482:484	ECD	482:484	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	0	79	theme	Cell	104:107	arg1	Trafficking					117:127	Notch Cell Surface Trafficking	98:127	Notch Cell Surface Trafficking	98:127	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	5	80	theme	type	1343:1346	arg1	cells					1348:1352	the wild type cells	1334:1352	the wild type cells	1334:1352	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	6	81	from	ECDs	1612:1615	arg1	control					1632:1638	the quality control	1620:1638	the quality control of Notch receptors	1620:1657	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	2	82	theme	Notch	398:402	arg1	receptors					404:412	Notch receptors	398:412	Notch receptors	398:412	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	2	82	theme	Notch	398:402	arg1	proteins					439:446	type-I transmembrane proteins	418:446	type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats	418:546	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	5	83	theme	wild	1338:1341	arg1	cells					1348:1352	the wild type cells	1334:1352	the wild type cells	1334:1352	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	0	84	theme	O-Glucose	21:29	arg1	Glycans					31:37	O-Glucose Glycans	21:37	O-Glucose Glycans	21:37	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	6	85	theme	double	1477:1482	arg1	cells					1493:1497	GXYLT1 and GXYLT2 double knockout cells	1459:1497	GXYLT1 and GXYLT2 double knockout cells	1459:1497	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	3	86	theme	repeats	841:847	arg1	structures					702:711	the structures	698:711	the structures	698:711	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	86	theme	repeats	841:847	arg1	stoichiometries					717:731	stoichiometries	717:731	stoichiometries	717:731	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	4	87	theme	xylosyl	929:935	arg1	elongation					937:946	xylosyl elongation	929:946	xylosyl elongation	929:946	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	6	88	theme	Notch	1643:1647	arg1	receptors					1649:1657	Notch receptors	1643:1657	Notch receptors	1643:1657	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	5	89	theme	HEK293T	1205:1211	arg1	cells					1213:1217	HEK293T cells	1205:1217	HEK293T cells	1205:1217	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	2	90	theme	transmembrane	425:437	arg1	receptors					404:412	Notch receptors	398:412	Notch receptors	398:412	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	2	90	theme	transmembrane	425:437	arg1	proteins					439:446	type-I transmembrane proteins	418:446	type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats	418:546	Notch receptors are type-I transmembrane proteins with large extracellular domains (ECD), containing 29-36 epidermal growth factor-like (EGF) repeats.					
32423029	0	91	theme	Extracellular	46:58	arg1	NOTCH1					70:75	NOTCH1	70:75	NOTCH1	70:75	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	0	91	theme	Extracellular	46:58	arg1	Domain					60:65	the Extracellular Domain	42:65	the Extracellular Domain of NOTCH1 and NOTCH2	42:86	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	0	91	theme	Extracellular	46:58	arg1	NOTCH2					81:86	NOTCH2	81:86	NOTCH2	81:86	Xylosyl Extension of O-Glucose Glycans on the Extracellular Domain of NOTCH1 and NOTCH2 Regulates Notch Cell Surface Trafficking.					
32423029	3	92	theme	HEK293T	615:621	arg1	cells					623:627	HEK293T cells	615:627	HEK293T cells using an Orbitrap Fusion mass spectrometer	615:670	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	5	93	theme	overexpressed	1270:1282	arg1	NOTCH1					1284:1289	overexpressed NOTCH1	1270:1289	overexpressed NOTCH1	1270:1289	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	5	94	theme	responsible	1061:1071	arg1	xylosyltransferases					1021:1039	both glucoside xylosyltransferases	1006:1039	both glucoside xylosyltransferases	1006:1039	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	5	94	theme	responsible	1061:1071	arg1	GXYLT2					1053:1058	GXYLT2	1053:1058	GXYLT2	1053:1058	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	5	94	theme	responsible	1061:1071	arg1	GXYLT1					1042:1047	GXYLT1	1042:1047	GXYLT1	1042:1047	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
32423029	3	95	theme	NOTCH2	852:857	arg1	repeats					747:753	all 17 EGF repeats	736:753	all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2)	736:817	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	3	95	theme	NOTCH2	852:857	arg1	repeats					841:847	16 out of 17 EGF repeats	824:847	16 out of 17 EGF repeats of NOTCH2	824:857	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	4	96	theme	NOTCH1	970:975	arg1	repeats					992:998	most NOTCH1 and NOTCH2 EGF repeats	965:998	most NOTCH1 and NOTCH2 EGF repeats	965:998	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	6	97	dep	In	1355:1356	arg1	vitro					1358:1362	vitro	1358:1362	vitro	1358:1362	In vitro secretion assays consistently showed a reduced secretion of both the NOTCH1 and NOTCH2 ECDs in GXYLT1 and GXYLT2 double knockout cells compared with the wild type cells, suggesting a significant role of the elongation of O-Glc glycans on the Notch ECDs in the quality control of Notch receptors.					
32423029	4	98	theme	NOTCH2	981:986	arg1	repeats					992:998	most NOTCH1 and NOTCH2 EGF repeats	965:998	most NOTCH1 and NOTCH2 EGF repeats	965:998	High levels of O-Glc attachment and xylosyl elongation were detected on most NOTCH1 and NOTCH2 EGF repeats.					
32423029	3	99	theme	Orbitrap	638:645	arg1	spectrometer					659:670	an Orbitrap Fusion mass spectrometer	635:670	an Orbitrap Fusion mass spectrometer	635:670	Here, we analyzed O-Glc glycans on NOTCH1 and NOTCH2 expressed in HEK293T cells using an Orbitrap Fusion mass spectrometer and successfully revealed the structures and stoichiometries of all 17 EGF repeats of NOTCH1 with the O-Glc consensus sequence (C1-X-S-X-(P/A)-C2), and 16 out of 17 EGF repeats of NOTCH2 with the same consensus sequence.					
32423029	5	100	theme	cell	1243:1246	arg1	expression					1256:1265	the cell surface expression	1239:1265	the cell surface expression of overexpressed NOTCH1 and NOTCH2	1239:1300	When both glucoside xylosyltransferases, GXYLT1 and GXYLT2, responsible for the xylosyl elongation of O-glucose, were genetically deleted, the expression of endogenous NOTCH1 and NOTCH2 on the surface of HEK293T cells did not change, but the cell surface expression of overexpressed NOTCH1 and NOTCH2 decreased compared with that in the wild type cells.					
33260488	6	0	from	experiments	929:939	arg1	cells					944:948	cells	944:948	cells expressing or lacking cell surface integrins of the β2 family	944:1010	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	4	1	gly	deglycosylation	637:651	arg1	structures					669:678	cell surface structures	656:678	cell surface structures	656:678	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	6	2	theme	kingae	1078:1083	arg1	RtxA					1085:1088	K. kingae RtxA	1075:1088	K. kingae RtxA	1075:1088	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	8	3	theme	hemolysins	1481:1490	arg1	hemolysins					1481:1490	broadly cytolytic RTX hemolysins	1459:1490	broadly cytolytic RTX hemolysins	1459:1490	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	8	3	theme	hemolysins	1481:1490	arg1	group					1450:1454	the group	1446:1454	the group of broadly cytolytic RTX hemolysins	1446:1490	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	6	4	theme	family	1005:1010	arg1	integrins					985:993	cell surface integrins	972:993	cell surface integrins of the β2 family	972:1010	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	7	5	attach	present	1210:1216	arg2	oligosaccharides					1193:1208	cell surface oligosaccharides	1180:1208	cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins	1180:1285	Our results, hence, show that RtxA binds cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins.					
33260488	7	5	attach	present	1210:1216	arg1	integrins					1277:1285	the leukocyte-restricted β2 integrins	1249:1285	all mammalian cells but not the leukocyte-restricted β2 integrins	1221:1285	Our results, hence, show that RtxA binds cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins.					
33260488	7	5	attach	present	1210:1216	arg1	cells					1235:1239	all mammalian cells	1221:1239	all mammalian cells but not the leukocyte-restricted β2 integrins	1221:1285	Our results, hence, show that RtxA binds cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins.					
33260488	3	6	theme	RtxA	503:506	arg1	interaction					488:498	interaction	488:498	interaction of RtxA with target cells	488:524	Recently, we demonstrated that membrane cholesterol facilitates interaction of RtxA with target cells, but other cell surface structures potentially involved in toxin binding to cells remain unknown.					
33260488	1	7	theme	skeletal	258:265	arg1	infections					274:283	skeletal system infections	258:283	skeletal system infections	258:283	The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections.					
33260488	4	8	theme	chemical	757:764	arg1	inhibitors					766:775	chemical inhibitors	757:775	chemical inhibitors	757:775	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	6	9	theme	other	1039:1043	arg1	cytotoxins					1045:1054	some other cytotoxins	1034:1054	some other cytotoxins of the RTX family	1034:1072	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	1	10	theme	system	267:272	arg1	infections					274:283	skeletal system infections	258:283	skeletal system infections	258:283	The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections.					
33260488	8	11	theme	toxin	1345:1349	arg1	interaction					1326:1336	the previously observed interaction	1302:1336	the previously observed interaction of the toxin with a broad range of cell types of various mammalian species	1302:1411	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	6	12	theme	β2	1125:1126	arg1	integrins					1128:1136	the β2 integrins	1121:1136	the β2 integrins	1121:1136	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	7	13	theme	present	1210:1216	arg1	oligosaccharides					1193:1208	cell surface oligosaccharides	1180:1208	cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins	1180:1285	Our results, hence, show that RtxA binds cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins.					
33260488	3	14	theme	other	531:535	arg1	structures					550:559	other cell surface structures	531:559	other cell surface structures potentially involved in toxin binding to cells	531:606	Recently, we demonstrated that membrane cholesterol facilitates interaction of RtxA with target cells, but other cell surface structures potentially involved in toxin binding to cells remain unknown.					
33260488	5	15	theme	deglycosylated	847:860	arg1	cells					862:866	the deglycosylated cells	843:866	the deglycosylated cells	843:866	Consequently, the deglycosylated cells were more resistant to cytotoxic activity of RtxA.					
33260488	5	15	theme	deglycosylated	847:860	arg1	resistant					878:886	resistant	878:886	resistant	878:886	Consequently, the deglycosylated cells were more resistant to cytotoxic activity of RtxA.					
33260488	0	16	theme	β2	91:92	arg1	Integrins					94:102	β2 Integrins	91:102	β2 Integrins	91:102	Binding of Kingella kingae RtxA Toxin Depends on Cell Surface Oligosaccharides, but Not on β2 Integrins.					
33260488	8	17	theme	RTX	1477:1479	arg1	hemolysins					1481:1490	broadly cytolytic RTX hemolysins	1459:1490	broadly cytolytic RTX hemolysins	1459:1490	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	4	18	theme	surface	661:667	arg1	structures					669:678	cell surface structures	656:678	cell surface structures	656:678	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	2	19	theme	ToXin	373:377	arg1	cytotoxins					380:389	the RTX (Repeats in ToXin) cytotoxins	353:389	the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens	353:421	K. kingae secretes an RtxA toxin that belongs to a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens.					
33260488	5	20	theme	RtxA	913:916	arg1	activity					901:908	cytotoxic activity	891:908	cytotoxic activity of RtxA	891:916	Consequently, the deglycosylated cells were more resistant to cytotoxic activity of RtxA.					
33260488	8	21	with	interaction	1326:1336	arg1	range					1364:1368	a broad range	1356:1368	a broad range of cell types of various mammalian species	1356:1411	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	6	22	theme	K.	1075:1076	arg1	RtxA					1085:1088	K. kingae RtxA	1075:1088	K. kingae RtxA	1075:1088	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	2	23	theme	in	370:371	arg1	cytotoxins					380:389	the RTX (Repeats in ToXin) cytotoxins	353:389	the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens	353:421	K. kingae secretes an RtxA toxin that belongs to a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens.					
33260488	8	24	theme	broad	1358:1362	arg1	range					1364:1368	a broad range	1356:1368	a broad range of cell types of various mammalian species	1356:1411	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	7	25	theme	cell	1180:1183	arg1	oligosaccharides					1193:1208	cell surface oligosaccharides	1180:1208	cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins	1180:1285	Our results, hence, show that RtxA binds cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins.					
33260488	4	26	theme	N-	731:732	arg1	treatment					695:703	glycosidase treatment	683:703	glycosidase treatment	683:703	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	4	26	theme	N-	731:732	arg1	inhibition					709:718	inhibition	709:718	inhibition of protein N- and O-glycosylation by chemical inhibitors	709:775	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	6	27	theme	RTX	1063:1065	arg1	family					1067:1072	the RTX family	1059:1072	the RTX family	1059:1072	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	2	28	theme	Repeats	362:368	arg1	cytotoxins					380:389	the RTX (Repeats in ToXin) cytotoxins	353:389	the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens	353:421	K. kingae secretes an RtxA toxin that belongs to a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens.					
33260488	8	29	theme	cytolytic	1467:1475	arg1	hemolysins					1481:1490	broadly cytolytic RTX hemolysins	1459:1490	broadly cytolytic RTX hemolysins	1459:1490	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	3	30	with	interaction	488:498	arg1	cells					520:524	target cells	513:524	target cells	513:524	Recently, we demonstrated that membrane cholesterol facilitates interaction of RtxA with target cells, but other cell surface structures potentially involved in toxin binding to cells remain unknown.					
33260488	4	31	theme	protein	723:729	arg1	N-					731:732	protein N-	723:732	protein N-	723:732	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	4	32	theme	glycosidase	683:693	arg1	treatment					695:703	glycosidase treatment	683:703	glycosidase treatment	683:703	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	0	33	theme	kingae	20:25	arg1	Toxin					32:36	Kingella kingae RtxA Toxin	11:36	Kingella kingae RtxA Toxin	11:36	Binding of Kingella kingae RtxA Toxin Depends on Cell Surface Oligosaccharides, but Not on β2 Integrins.					
33260488	6	34	theme	surface	977:983	arg1	integrins					985:993	cell surface integrins	972:993	cell surface integrins of the β2 family	972:1010	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	3	35	theme	cell	537:540	arg1	structures					550:559	other cell surface structures	531:559	other cell surface structures potentially involved in toxin binding to cells	531:606	Recently, we demonstrated that membrane cholesterol facilitates interaction of RtxA with target cells, but other cell surface structures potentially involved in toxin binding to cells remain unknown.					
33260488	1	36	theme	important	186:194	arg1	coccobacillus					123:135	The Gram-negative coccobacillus	105:135	The Gram-negative coccobacillus Kingella kingae	105:151	The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections.					
33260488	1	36	theme	important	186:194	arg1	pathogen					215:222	an important invasive pediatric pathogen	183:222	an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections	183:283	The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections.					
33260488	2	37	theme	bacterial	403:411	arg1	pathogens					413:421	bacterial pathogens	403:421	bacterial pathogens	403:421	K. kingae secretes an RtxA toxin that belongs to a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens.					
33260488	6	38	theme	cell	972:975	arg1	integrins					985:993	cell surface integrins	972:993	cell surface integrins of the β2 family	972:1010	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	6	39	dep	cells	944:948	arg1	expressing					950:959	expressing	950:959	expressing	950:959	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	6	39	dep	cells	944:948	arg1	lacking					964:970	lacking	964:970	lacking cell surface integrins of the β2 family	964:1010	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	3	40	theme	surface	542:548	arg1	structures					550:559	other cell surface structures	531:559	other cell surface structures potentially involved in toxin binding to cells	531:606	Recently, we demonstrated that membrane cholesterol facilitates interaction of RtxA with target cells, but other cell surface structures potentially involved in toxin binding to cells remain unknown.					
33260488	1	41	theme	invasive	196:203	arg1	coccobacillus					123:135	The Gram-negative coccobacillus	105:135	The Gram-negative coccobacillus Kingella kingae	105:151	The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections.					
33260488	1	41	theme	invasive	196:203	arg1	pathogen					215:222	an important invasive pediatric pathogen	183:222	an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections	183:283	The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections.					
33260488	0	42	theme	Toxin	32:36	arg1	Binding					0:6	Binding	0:6	Binding of Kingella kingae RtxA Toxin	0:36	Binding of Kingella kingae RtxA Toxin Depends on Cell Surface Oligosaccharides, but Not on β2 Integrins.					
33260488	1	43	theme	pediatric	205:213	arg1	coccobacillus					123:135	The Gram-negative coccobacillus	105:135	The Gram-negative coccobacillus Kingella kingae	105:151	The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections.					
33260488	1	43	theme	pediatric	205:213	arg1	pathogen					215:222	an important invasive pediatric pathogen	183:222	an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections	183:283	The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections.					
33260488	3	44	theme	toxin	585:589	arg1	binding					591:597	toxin binding	585:597	toxin binding to cells	585:606	Recently, we demonstrated that membrane cholesterol facilitates interaction of RtxA with target cells, but other cell surface structures potentially involved in toxin binding to cells remain unknown.					
33260488	4	45	theme	cell	656:659	arg1	structures					669:678	cell surface structures	656:678	cell surface structures	656:678	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	2	46	theme	cytotoxins	380:389	arg1	family					343:348	a broad family	335:348	a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens	335:421	K. kingae secretes an RtxA toxin that belongs to a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens.					
33260488	0	47	theme	RtxA	27:30	arg1	Toxin					32:36	Kingella kingae RtxA Toxin	11:36	Kingella kingae RtxA Toxin	11:36	Binding of Kingella kingae RtxA Toxin Depends on Cell Surface Oligosaccharides, but Not on β2 Integrins.					
33260488	6	48	theme	β2	1002:1003	arg1	family					1005:1010	the β2 family	998:1010	the β2 family	998:1010	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	4	49	theme	target	815:820	arg1	cells					822:826	target cells	815:826	target cells	815:826	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	4	50	theme	O-glycosylation	738:752	arg1	treatment					695:703	glycosidase treatment	683:703	glycosidase treatment	683:703	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	4	50	theme	O-glycosylation	738:752	arg1	inhibition					709:718	inhibition	709:718	inhibition of protein N- and O-glycosylation by chemical inhibitors	709:775	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	5	51	theme	cytotoxic	891:899	arg1	activity					901:908	cytotoxic activity	891:908	cytotoxic activity of RtxA	891:916	Consequently, the deglycosylated cells were more resistant to cytotoxic activity of RtxA.					
33260488	8	52	theme	observed	1317:1324	arg1	interaction					1326:1336	the previously observed interaction	1302:1336	the previously observed interaction of the toxin with a broad range of cell types of various mammalian species	1302:1411	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	7	53	theme	surface	1185:1191	arg1	oligosaccharides					1193:1208	cell surface oligosaccharides	1180:1208	cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins	1180:1285	Our results, hence, show that RtxA binds cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins.					
33260488	8	54	theme	cell	1373:1376	arg1	types					1378:1382	cell types	1373:1382	cell types of various mammalian species	1373:1411	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	2	55	theme	broad	337:341	arg1	family					343:348	a broad family	335:348	a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens	335:421	K. kingae secretes an RtxA toxin that belongs to a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens.					
33260488	5	56	gly	deglycosylated	847:860	arg1	cells					862:866	the deglycosylated cells	843:866	the deglycosylated cells	843:866	Consequently, the deglycosylated cells were more resistant to cytotoxic activity of RtxA.					
33260488	5	56	gly	deglycosylated	847:860	arg1	resistant					878:886	resistant	878:886	resistant	878:886	Consequently, the deglycosylated cells were more resistant to cytotoxic activity of RtxA.					
33260488	6	57	theme	target	1104:1109	arg1	cells					1111:1115	target cells	1104:1115	target cells	1104:1115	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	8	58	theme	species	1405:1411	arg1	types					1378:1382	cell types	1373:1382	cell types of various mammalian species	1373:1411	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	8	59	theme	various	1387:1393	arg1	species					1405:1411	various mammalian species	1387:1411	various mammalian species	1387:1411	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	4	60	theme	RtxA	799:802	arg1	binding					804:810	RtxA binding	799:810	RtxA binding to target cells	799:826	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	4	61	theme	structures	669:678	arg1	deglycosylation					637:651	deglycosylation	637:651	deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors	637:775	We show that deglycosylation of cell surface structures by glycosidase treatment, or inhibition of protein N- and O-glycosylation by chemical inhibitors substantially reduces RtxA binding to target cells.					
33260488	3	62	theme	membrane	455:462	arg1	cholesterol					464:474	membrane cholesterol	455:474	membrane cholesterol	455:474	Recently, we demonstrated that membrane cholesterol facilitates interaction of RtxA with target cells, but other cell surface structures potentially involved in toxin binding to cells remain unknown.					
33260488	2	63	theme	RtxA	308:311	arg1	toxin					313:317	an RtxA toxin	305:317	an RtxA toxin that belongs to a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens	305:421	K. kingae secretes an RtxA toxin that belongs to a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens.					
33260488	8	64	theme	types	1378:1382	arg1	range					1364:1368	a broad range	1356:1368	a broad range of cell types of various mammalian species	1356:1411	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	1	65	theme	Gram-negative	109:121	arg1	coccobacillus					123:135	The Gram-negative coccobacillus	105:135	The Gram-negative coccobacillus Kingella kingae	105:151	The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections.					
33260488	1	65	theme	Gram-negative	109:121	arg1	pathogen					215:222	an important invasive pediatric pathogen	183:222	an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections	183:283	The Gram-negative coccobacillus Kingella kingae is increasingly recognized as an important invasive pediatric pathogen that causes mostly bacteremia and skeletal system infections.					
33260488	7	66	theme	mammalian	1225:1233	arg1	cells					1235:1239	all mammalian cells	1221:1239	all mammalian cells but not the leukocyte-restricted β2 integrins	1221:1285	Our results, hence, show that RtxA binds cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins.					
33260488	0	67	theme	Surface	54:60	arg1	Oligosaccharides					62:77	Cell Surface Oligosaccharides	49:77	Cell Surface Oligosaccharides	49:77	Binding of Kingella kingae RtxA Toxin Depends on Cell Surface Oligosaccharides, but Not on β2 Integrins.					
33260488	7	68	theme	leukocyte-restricted	1253:1272	arg1	integrins					1277:1285	the leukocyte-restricted β2 integrins	1249:1285	all mammalian cells but not the leukocyte-restricted β2 integrins	1221:1285	Our results, hence, show that RtxA binds cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins.					
33260488	8	69	theme	mammalian	1395:1403	arg1	species					1405:1411	various mammalian species	1387:1411	various mammalian species	1387:1411	This explains the previously observed interaction of the toxin with a broad range of cell types of various mammalian species and reveals that RtxA belongs to the group of broadly cytolytic RTX hemolysins.					
33260488	7	70	theme	β2	1274:1275	arg1	integrins					1277:1285	the leukocyte-restricted β2 integrins	1249:1285	all mammalian cells but not the leukocyte-restricted β2 integrins	1221:1285	Our results, hence, show that RtxA binds cell surface oligosaccharides present on all mammalian cells but not the leukocyte-restricted β2 integrins.					
33260488	2	71	theme	RTX	357:359	arg1	cytotoxins					380:389	the RTX (Repeats in ToXin) cytotoxins	353:389	the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens	353:421	K. kingae secretes an RtxA toxin that belongs to a broad family of the RTX (Repeats in ToXin) cytotoxins produced by bacterial pathogens.					
33260488	6	72	theme	family	1067:1072	arg1	cytotoxins					1045:1054	some other cytotoxins	1034:1054	some other cytotoxins of the RTX family	1034:1072	Moreover, experiments on cells expressing or lacking cell surface integrins of the β2 family revealed that, unlike some other cytotoxins of the RTX family, K. kingae RtxA does not bind target cells via the β2 integrins.					
33260488	0	73	theme	Cell	49:52	arg1	Oligosaccharides					62:77	Cell Surface Oligosaccharides	49:77	Cell Surface Oligosaccharides	49:77	Binding of Kingella kingae RtxA Toxin Depends on Cell Surface Oligosaccharides, but Not on β2 Integrins.					
33260488	0	74	theme	Kingella	11:18	arg1	Toxin					32:36	Kingella kingae RtxA Toxin	11:36	Kingella kingae RtxA Toxin	11:36	Binding of Kingella kingae RtxA Toxin Depends on Cell Surface Oligosaccharides, but Not on β2 Integrins.					
33260488	3	75	theme	target	513:518	arg1	cells					520:524	target cells	513:524	target cells	513:524	Recently, we demonstrated that membrane cholesterol facilitates interaction of RtxA with target cells, but other cell surface structures potentially involved in toxin binding to cells remain unknown.					
31643054	1	0	theme	interesting	75:85	arg1	Fc-glycans					60:69	The antibody Fc-glycans	47:69	The antibody Fc-glycans	47:69	The antibody Fc-glycans are interesting targets for conjugation of cytotoxic compounds due to their localization and their chemical composition.					
31643054	1	0	theme	interesting	75:85	arg1	targets					87:93	interesting targets	75:93	interesting targets for conjugation of cytotoxic compounds due to their localization and their chemical composition	75:189	The antibody Fc-glycans are interesting targets for conjugation of cytotoxic compounds due to their localization and their chemical composition.					
31643054	3	1	theme	enzymatic	328:336	arg1	methodology					338:348	a two-step enzymatic methodology	317:348	a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo	317:572	Here we present a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo.					
31643054	3	2	theme	anti-tumor	529:538	arg1	effects					540:546	cytotoxic anti-tumor effects	519:546	cytotoxic anti-tumor effects	519:546	Here we present a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo.					
31643054	1	3	theme	cytotoxic	114:122	arg1	compounds					124:132	cytotoxic compounds	114:132	cytotoxic compounds	114:132	The antibody Fc-glycans are interesting targets for conjugation of cytotoxic compounds due to their localization and their chemical composition.					
31643054	3	4	from	GlcNAc	405:410	arg1	click-chemistry					361:375	click-chemistry	361:375	click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo	361:572	Here we present a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo.					
31643054	2	5	theme	site-specific	214:226	arg1	conjugates					228:237	site-specific conjugates	214:237	site-specific conjugates	214:237	In striving to obtain site-specific conjugates, the antibody Fc-glycans have been explored in numerous ways.					
31643054	2	6	theme	numerous	286:293	arg1	ways					295:298	numerous ways	286:298	numerous ways	286:298	In striving to obtain site-specific conjugates, the antibody Fc-glycans have been explored in numerous ways.					
31643054	2	7	dep	obtain	207:212	arg1	striving					195:202	striving	195:202	striving	195:202	In striving to obtain site-specific conjugates, the antibody Fc-glycans have been explored in numerous ways.					
31643054	1	8	theme	chemical	170:177	arg1	composition					179:189	their chemical composition	164:189	their chemical composition	164:189	The antibody Fc-glycans are interesting targets for conjugation of cytotoxic compounds due to their localization and their chemical composition.					
31643054	3	9	with	homogenous	456:465	arg1	2.0					478:480	2.0	478:480	2.0	478:480	Here we present a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo.					
31643054	3	10	theme	Fc-glycan	419:427	arg1	GlcNAc					405:410	the core GlcNAc	396:410	the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo	396:572	Here we present a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo.					
31643054	3	11	theme	two-step	319:326	arg1	methodology					338:348	a two-step enzymatic methodology	317:348	a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo	317:572	Here we present a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo.					
31643054	2	12	theme	antibody	244:251	arg1	Fc-glycans					253:262	the antibody Fc-glycans	240:262	the antibody Fc-glycans	240:262	In striving to obtain site-specific conjugates, the antibody Fc-glycans have been explored in numerous ways.					
31643054	3	13	theme	cytotoxic	519:527	arg1	effects					540:546	cytotoxic anti-tumor effects	519:546	cytotoxic anti-tumor effects	519:546	Here we present a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo.					
31643054	0	14	theme	Glycosyl	26:33	arg1	Remodeling					35:44	Glycosyl Remodeling	26:44	Glycosyl Remodeling	26:44	Antibody Conjugations via Glycosyl Remodeling.					
31643054	1	15	theme	compounds	124:132	arg1	conjugation					99:109	conjugation	99:109	conjugation of cytotoxic compounds due to their localization and their chemical composition	99:189	The antibody Fc-glycans are interesting targets for conjugation of cytotoxic compounds due to their localization and their chemical composition.					
31643054	1	16	theme	antibody	51:58	arg1	Fc-glycans					60:69	The antibody Fc-glycans	47:69	The antibody Fc-glycans	47:69	The antibody Fc-glycans are interesting targets for conjugation of cytotoxic compounds due to their localization and their chemical composition.					
31643054	1	16	theme	antibody	51:58	arg1	targets					87:93	interesting targets	75:93	interesting targets for conjugation of cytotoxic compounds due to their localization and their chemical composition	75:189	The antibody Fc-glycans are interesting targets for conjugation of cytotoxic compounds due to their localization and their chemical composition.					
31643054	3	17	theme	antigen	490:496	arg1	binding					498:504	antigen binding	490:504	antigen binding	490:504	Here we present a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo.					
31643054	3	18	theme	core	400:403	arg1	GlcNAc					405:410	the core GlcNAc	396:410	the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo	396:572	Here we present a two-step enzymatic methodology coupled to click-chemistry for conjugation at the core GlcNAc of the Fc-glycan resulting in ADCs that are homogenous with a DAR 2.0, retain antigen binding, and display cytotoxic anti-tumor effects both in vitro and in vivo.					
32817270	5	0	theme	host	691:694	arg1	surface					701:707	the host cell surface	687:707	the host cell surface	687:707	We propose that the hinges allow S to scan the host cell surface, shielded from antibodies by an extensive glycan coat.					
32817270	6	1	theme	infection	837:845	arg1	understanding					809:821	our understanding	805:821	our understanding of SARS-CoV-2 infection	805:845	The structure of native S contributes to our understanding of SARS-CoV-2 infection and potentially to the development of safe vaccines.					
32817270	1	2	theme	acute	126:130	arg1	coronavirus					153:163	severe acute respiratory syndrome coronavirus 2	119:165	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	119:178	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	1	2	theme	acute	126:130	arg1	SARS-CoV-2					168:177	SARS-CoV-2	168:177	SARS-CoV-2	168:177	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	5	3	theme	cell	696:699	arg1	surface					701:707	the host cell surface	687:707	the host cell surface	687:707	We propose that the hinges allow S to scan the host cell surface, shielded from antibodies by an extensive glycan coat.					
32817270	6	4	theme	SARS-CoV-2	826:835	arg1	infection					837:845	SARS-CoV-2 infection	826:845	SARS-CoV-2 infection	826:845	The structure of native S contributes to our understanding of SARS-CoV-2 infection and potentially to the development of safe vaccines.					
32817270	1	5	theme	respiratory	132:142	arg1	coronavirus					153:163	severe acute respiratory syndrome coronavirus 2	119:165	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	119:178	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	1	5	theme	respiratory	132:142	arg1	SARS-CoV-2					168:177	SARS-CoV-2	168:177	SARS-CoV-2	168:177	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	3	6	gly	glycosylated	465:476	arg1	S					446:446	the viral S	436:446	the viral S	436:446	Compared with the recombinant S, the viral S was more heavily glycosylated and occurred mostly in the closed prefusion conformation.					
32817270	1	7	theme	spike	98:102	arg1	S					113:113	The spike protein (S)	94:114	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	94:178	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	1	8	theme	primary	218:224	arg1	focus					226:230	the primary focus	214:230	the primary focus for vaccine development	214:254	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	6	9	theme	vaccines	890:897	arg1	development					870:880	the development	866:880	the development of safe vaccines	866:897	The structure of native S contributes to our understanding of SARS-CoV-2 infection and potentially to the development of safe vaccines.					
32817270	2	10	theme	subtomogram	310:320	arg1	averaging					322:330	subtomogram averaging	310:330	subtomogram averaging	310:330	In this study, we combined cryo-electron tomography, subtomogram averaging, and molecular dynamics simulations to structurally analyze S in situ.					
32817270	3	11	theme	viral	440:444	arg1	S					446:446	the viral S	436:446	the viral S	436:446	Compared with the recombinant S, the viral S was more heavily glycosylated and occurred mostly in the closed prefusion conformation.					
32817270	4	12	theme	head	605:608	arg1	freedom					635:641	the head unexpected orientational freedom	601:641	the head unexpected orientational freedom	601:641	We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom.					
32817270	1	13	theme	syndrome	144:151	arg1	coronavirus					153:163	severe acute respiratory syndrome coronavirus 2	119:165	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	119:178	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	1	13	theme	syndrome	144:151	arg1	SARS-CoV-2					168:177	SARS-CoV-2	168:177	SARS-CoV-2	168:177	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	1	14	theme	coronavirus	153:163	arg1	S					113:113	The spike protein (S)	94:114	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	94:178	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	3	15	theme	prefusion	512:520	arg1	conformation					522:533	the closed prefusion conformation	501:533	the closed prefusion conformation	501:533	Compared with the recombinant S, the viral S was more heavily glycosylated and occurred mostly in the closed prefusion conformation.					
32817270	2	16	theme	molecular	337:345	arg1	simulations					356:366	molecular dynamics simulations	337:366	molecular dynamics simulations	337:366	In this study, we combined cryo-electron tomography, subtomogram averaging, and molecular dynamics simulations to structurally analyze S in situ.					
32817270	0	17	theme	In	0:1	arg1	analysis					19:26	In situ structural analysis	0:26	In situ structural analysis of SARS-CoV-2 spike	0:46	In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.					
32817270	1	18	theme	vaccine	236:242	arg1	development					244:254	vaccine development	236:254	vaccine development	236:254	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	3	19	theme	recombinant	421:431	arg1	S					433:433	the recombinant S	417:433	the recombinant S	417:433	Compared with the recombinant S, the viral S was more heavily glycosylated and occurred mostly in the closed prefusion conformation.					
32817270	0	20	theme	structural	8:17	arg1	analysis					19:26	In situ structural analysis	0:26	In situ structural analysis of SARS-CoV-2 spike	0:46	In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.					
32817270	6	21	theme	safe	885:888	arg1	vaccines					890:897	safe vaccines	885:897	safe vaccines	885:897	The structure of native S contributes to our understanding of SARS-CoV-2 infection and potentially to the development of safe vaccines.					
32817270	6	22	theme	S	788:788	arg1	structure					768:776	The structure	764:776	The structure of native S	764:788	The structure of native S contributes to our understanding of SARS-CoV-2 infection and potentially to the development of safe vaccines.					
32817270	3	23	theme	closed	505:510	arg1	conformation					522:533	the closed prefusion conformation	501:533	the closed prefusion conformation	501:533	Compared with the recombinant S, the viral S was more heavily glycosylated and occurred mostly in the closed prefusion conformation.					
32817270	4	24	theme	S	569:569	arg1	S					569:569	S	569:569	S	569:569	We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom.					
32817270	4	24	theme	S	569:569	arg1	domain					559:564	the stalk domain	549:564	the stalk domain of S	549:569	We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom.					
32817270	0	25	theme	SARS-CoV-2	31:40	arg1	spike					42:46	SARS-CoV-2 spike	31:46	SARS-CoV-2 spike	31:46	In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.					
32817270	5	26	dep	scan	682:685	arg1	shielded					710:717	shielded	710:717	shielded from antibodies by an extensive glycan coat	710:761	We propose that the hinges allow S to scan the host cell surface, shielded from antibodies by an extensive glycan coat.					
32817270	6	27	theme	native	781:786	arg1	S					788:788	native S	781:788	native S	781:788	The structure of native S contributes to our understanding of SARS-CoV-2 infection and potentially to the development of safe vaccines.					
32817270	4	28	contain	contains	571:578	arg2	hinges					586:591	three hinges	580:591	three hinges	580:591	We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom.					
32817270	4	28	contain	contains	571:578	arg1	S					569:569	S	569:569	S	569:569	We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom.					
32817270	4	28	contain	contains	571:578	arg1	domain					559:564	the stalk domain	549:564	the stalk domain of S	549:569	We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom.					
32817270	1	29	theme	protein	104:110	arg1	S					113:113	The spike protein (S)	94:114	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	94:178	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	0	30	theme	spike	42:46	arg1	analysis					19:26	In situ structural analysis	0:26	In situ structural analysis of SARS-CoV-2 spike	0:46	In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.					
32817270	2	31	theme	cryo-electron	284:296	arg1	tomography					298:307	cryo-electron tomography	284:307	cryo-electron tomography	284:307	In this study, we combined cryo-electron tomography, subtomogram averaging, and molecular dynamics simulations to structurally analyze S in situ.					
32817270	4	32	theme	stalk	553:557	arg1	S					569:569	S	569:569	S	569:569	We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom.					
32817270	4	32	theme	stalk	553:557	arg1	domain					559:564	the stalk domain	549:564	the stalk domain of S	549:569	We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom.					
32817270	5	33	theme	extensive	741:749	arg1	coat					758:761	an extensive glycan coat	738:761	an extensive glycan coat	738:761	We propose that the hinges allow S to scan the host cell surface, shielded from antibodies by an extensive glycan coat.					
32817270	4	34	theme	unexpected	610:619	arg1	freedom					635:641	the head unexpected orientational freedom	601:641	the head unexpected orientational freedom	601:641	We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom.					
32817270	1	35	theme	cell	196:199	arg1	entry					201:205	cell entry	196:205	cell entry	196:205	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	5	36	theme	glycan	751:756	arg1	coat					758:761	an extensive glycan coat	738:761	an extensive glycan coat	738:761	We propose that the hinges allow S to scan the host cell surface, shielded from antibodies by an extensive glycan coat.					
32817270	4	37	theme	orientational	621:633	arg1	freedom					635:641	the head unexpected orientational freedom	601:641	the head unexpected orientational freedom	601:641	We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom.					
32817270	2	38	theme	dynamics	347:354	arg1	simulations					356:366	molecular dynamics simulations	337:366	molecular dynamics simulations	337:366	In this study, we combined cryo-electron tomography, subtomogram averaging, and molecular dynamics simulations to structurally analyze S in situ.					
32817270	0	39	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.					
32817270	1	40	theme	severe	119:124	arg1	coronavirus					153:163	severe acute respiratory syndrome coronavirus 2	119:165	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	119:178	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32817270	1	40	theme	severe	119:124	arg1	SARS-CoV-2					168:177	SARS-CoV-2	168:177	SARS-CoV-2	168:177	The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development.					
32205858	10	0	theme	based	1576:1580	arg1	method					1605:1610	a novel in situ proximity-ligation based histochemical staining method	1541:1610	a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin	1541:1663	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	3	1	theme	normal	522:527	arg1	endometrium					529:539	a normal endometrium	520:539	a normal endometrium	520:539	Here we aimed to elucidate whether the glycosylation and function of glycodelin is altered in endometrial carcinoma as compared with a normal endometrium.					
32205858	9	2	theme	mononuclear	1443:1453	arg1	cell					1455:1458	peripheral blood mononuclear cell	1426:1458	peripheral blood mononuclear cell proliferation	1426:1472	Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation.					
32205858	4	3	theme	human	617:621	arg1	cells					645:649	HEC-1B human endometrial carcinoma cells	610:649	HEC-1B human endometrial carcinoma cells (HEC-1B Gd)	610:661	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	4	3	theme	human	617:621	arg1	Gd					659:660	HEC-1B Gd	652:660	HEC-1B Gd	652:660	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	10	4	theme	specific	1620:1627	arg1	antibody					1640:1647	a specific glycodelin antibody	1618:1647	a specific glycodelin antibody	1618:1647	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	7	5	theme	poly-LacNAc	1136:1146	arg1	antennae					1148:1155	more abundant poly-LacNAc antennae	1122:1155	more abundant poly-LacNAc antennae	1122:1155	These included a lower level of sialylation and more abundant poly-LacNAc antennae, some of which are fucosylated.					
32205858	4	6	contain	carried	545:551	arg1	We					542:543	We	542:543	We	542:543	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	4	6	contain	carried	545:551	arg2	analysis					574:581	glycan structure analysis	557:581	glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd)	557:661	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	2	7	contain	has	180:182	arg1	It					177:178	It	177:178	It	177:178	It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype.					
32205858	2	7	contain	has	180:182	arg2	functions					213:221	several reproduction related functions	184:221	several reproduction related functions that are dependent on specific glycosylation	184:266	It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype.					
32205858	6	8	theme	several	892:898	arg1	differences					900:910	several differences	892:910	several differences	892:910	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	10	9	theme	UEAI	1653:1656	arg1	lectin					1658:1663	UEAI lectin	1653:1663	UEAI lectin	1653:1663	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	7	10	theme	sialylation	1106:1116	arg1	level					1097:1101	a lower level	1089:1101	a lower level	1089:1101	These included a lower level of sialylation and more abundant poly-LacNAc antennae, some of which are fucosylated.					
32205858	6	11	theme	endometrium-derived	997:1015	arg1	glycodelin-A					1037:1048	glycodelin-A	1037:1048	glycodelin-A (GdA)	1037:1054	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	6	11	theme	endometrium-derived	997:1015	arg1	isoform					1028:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	6	12	theme	human	978:982	arg1	glycodelin-A					1037:1048	glycodelin-A	1037:1048	glycodelin-A (GdA)	1037:1054	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	6	12	theme	human	978:982	arg1	isoform					1028:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	5	13	contain	containing	827:836	arg1	mixture					760:766	a typical mixture	750:766	a typical mixture	750:766	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	5	13	contain	containing	827:836	arg2	antennae					873:880	undecorated LacNAc (Galβ1-4GlcNAc) antennae	838:880	undecorated LacNAc (Galβ1-4GlcNAc) antennae	838:880	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	13	14	used	used	2097:2100	arg2	methods					2082:2088	Similar methods	2074:2088	Similar methods	2074:2088	Similar methods can be used for studies of other glycoproteins.					
32205858	2	15	theme	specific	245:252	arg1	glycosylation					254:266	specific glycosylation	245:266	specific glycosylation	245:266	It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype.					
32205858	9	16	from	differences	1297:1307	arg1	glycosylation					1312:1324	glycosylation	1312:1324	glycosylation	1312:1324	Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation.					
32205858	11	17	from	tissue	1791:1796	arg1	abundant					1713:1720	abundant	1713:1720	abundant	1713:1720	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	11	17	from	tissue	1791:1796	arg1	glycodelin					1698:1707	the UEAI reactive glycodelin	1680:1707	the UEAI reactive glycodelin	1680:1707	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	6	18	link	endometrium-derived	997:1015	arg1	glycodelin-A					1037:1048	glycodelin-A	1037:1048	glycodelin-A (GdA)	1037:1054	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	6	18	link	endometrium-derived	997:1015	arg1	isoform					1028:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	6	19	theme	glycan	950:955	arg1	structures					957:966	previously reported glycan structures	930:966	previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA)	930:1054	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	5	20	theme	HEC-1B	717:722	arg1	Gd					724:725	HEC-1B Gd	717:725	HEC-1B Gd	717:725	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	5	21	theme	high-mannose	771:782	arg1	mixture					760:766	a typical mixture	750:766	a typical mixture	750:766	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	1	22	theme	decidualized	151:162	arg1	endometrium					164:174	decidualized endometrium	151:174	decidualized endometrium	151:174	Glycodelin is a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium.					
32205858	5	23	theme	hybrid	785:790	arg1	mixture					760:766	a typical mixture	750:766	a typical mixture	750:766	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	12	24	theme	specific	1991:1998	arg1	glycodelin					2000:2009	this specific glycodelin	1986:2009	this specific glycodelin	1986:2009	In conclusion, we established a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin and showed that this specific glycodelin is exclusively expressed in cancer, not in normal endometrium.					
32205858	11	25	from	carcinoma	1737:1745	arg1	abundant					1713:1720	abundant	1713:1720	abundant	1713:1720	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	11	25	from	carcinoma	1737:1745	arg1	glycodelin					1698:1707	the UEAI reactive glycodelin	1680:1707	the UEAI reactive glycodelin	1680:1707	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	1	26	gly	glycoprotein	84:95	arg1	Glycodelin					62:71	Glycodelin	62:71	Glycodelin	62:71	Glycodelin is a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium.					
32205858	1	26	gly	glycoprotein	84:95	arg1	glycoprotein					84:95	a major glycoprotein	76:95	a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium	76:174	Glycodelin is a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium.					
32205858	9	27	theme	HEC-1B	1334:1339	arg1	Gd					1341:1342	HEC-1B Gd	1334:1342	HEC-1B Gd	1334:1342	Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation.					
32205858	6	28	theme	isoform	1028:1034	arg1	structures					957:966	previously reported glycan structures	930:966	previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA)	930:1054	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	10	29	theme	glycodelin	1514:1523	arg1	detection					1483:1491	the detection	1479:1491	the detection of cancer associated glycodelin	1479:1523	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	13	30	theme	glycoproteins	2123:2135	arg1	studies					2106:2112	studies	2106:2112	studies of other glycoproteins	2106:2135	Similar methods can be used for studies of other glycoproteins.					
32205858	1	31	theme	major	78:82	arg1	Glycodelin					62:71	Glycodelin	62:71	Glycodelin	62:71	Glycodelin is a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium.					
32205858	1	31	theme	major	78:82	arg1	glycoprotein					84:95	a major glycoprotein	76:95	a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium	76:174	Glycodelin is a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium.					
32205858	10	32	theme	cancer	1496:1501	arg1	glycodelin					1514:1523	cancer associated glycodelin	1496:1523	cancer associated glycodelin	1496:1523	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	0	33	theme	Altered	0:6	arg1	glycosylation					8:20	Altered glycosylation	0:20	Altered glycosylation of glycodelin in endometrial carcinoma	0:59	Altered glycosylation of glycodelin in endometrial carcinoma.					
32205858	4	34	theme	structure	564:572	arg1	analysis					574:581	glycan structure analysis	557:581	glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd)	557:661	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	5	35	theme	undecorated	838:848	arg1	antennae					873:880	undecorated LacNAc (Galβ1-4GlcNAc) antennae	838:880	undecorated LacNAc (Galβ1-4GlcNAc) antennae	838:880	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	1	36	theme	reproductive	110:121	arg1	tissues					123:129	reproductive tissues	110:129	reproductive tissues	110:129	Glycodelin is a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium.					
32205858	2	37	theme	several	184:190	arg1	functions					213:221	several reproduction related functions	184:221	several reproduction related functions that are dependent on specific glycosylation	184:266	It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype.					
32205858	9	38	theme	inhibitory	1373:1382	arg1	activity					1384:1391	similar inhibitory activity	1365:1391	similar inhibitory activity	1365:1391	Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation.					
32205858	12	39	theme	carcinoma-associated	1938:1957	arg1	glycodelin					1959:1968	endometrial carcinoma-associated glycodelin	1926:1968	endometrial carcinoma-associated glycodelin	1926:1968	In conclusion, we established a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin and showed that this specific glycodelin is exclusively expressed in cancer, not in normal endometrium.					
32205858	0	40	gly	glycosylation	8:20	arg1	glycodelin					25:34	glycodelin	25:34	glycodelin	25:34	Altered glycosylation of glycodelin in endometrial carcinoma.					
32205858	0	40	gly	glycosylation	8:20	arg1	carcinoma					51:59	endometrial carcinoma	39:59	endometrial carcinoma	39:59	Altered glycosylation of glycodelin in endometrial carcinoma.					
32205858	10	41	theme	proximity-ligation	1557:1574	arg1	method					1605:1610	a novel in situ proximity-ligation based histochemical staining method	1541:1610	a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin	1541:1663	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	5	42	theme	N-glycans	810:818	arg1	mixture					760:766	a typical mixture	750:766	a typical mixture	750:766	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	12	43	theme	staining	1889:1896	arg1	method					1898:1903	a histochemical staining method	1873:1903	a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin	1873:1968	In conclusion, we established a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin and showed that this specific glycodelin is exclusively expressed in cancer, not in normal endometrium.					
32205858	10	44	theme	in	1549:1550	arg1	method					1605:1610	a novel in situ proximity-ligation based histochemical staining method	1541:1610	a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin	1541:1663	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	11	45	theme	endometrial	1779:1789	arg1	tissue					1791:1796	normal endometrial tissue	1772:1796	normal endometrial tissue	1772:1796	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	2	46	theme	cells	346:350	arg1	differentiation					305:319	differentiation	305:319	differentiation of endometrial carcinoma cells toward a less malignant phenotype	305:384	It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype.					
32205858	9	47	theme	cell	1408:1411	arg1	invasion					1413:1420	trophoblast cell invasion	1396:1420	trophoblast cell invasion	1396:1420	Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation.					
32205858	4	48	theme	endometrial	623:633	arg1	cells					645:649	HEC-1B human endometrial carcinoma cells	610:649	HEC-1B human endometrial carcinoma cells (HEC-1B Gd)	610:661	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	4	48	theme	endometrial	623:633	arg1	Gd					659:660	HEC-1B Gd	652:660	HEC-1B Gd	652:660	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	2	49	theme	related	205:211	arg1	functions					213:221	several reproduction related functions	184:221	several reproduction related functions that are dependent on specific glycosylation	184:266	It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype.					
32205858	13	50	theme	Similar	2074:2080	arg1	methods					2082:2088	Similar methods	2074:2088	Similar methods	2074:2088	Similar methods can be used for studies of other glycoproteins.					
32205858	6	51	theme	reported	941:948	arg1	structures					957:966	previously reported glycan structures	930:966	previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA)	930:1054	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	10	52	theme	histochemical	1582:1594	arg1	method					1605:1610	a novel in situ proximity-ligation based histochemical staining method	1541:1610	a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin	1541:1663	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	7	53	theme	antennae	1148:1155	arg1	level					1097:1101	a lower level	1089:1101	a lower level	1089:1101	These included a lower level of sialylation and more abundant poly-LacNAc antennae, some of which are fucosylated.					
32205858	2	54	theme	malignant	366:374	arg1	phenotype					376:384	a less malignant phenotype	359:384	a less malignant phenotype	359:384	It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype.					
32205858	9	55	theme	blood	1437:1441	arg1	cell					1455:1458	peripheral blood mononuclear cell	1426:1458	peripheral blood mononuclear cell proliferation	1426:1472	Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation.					
32205858	10	56	theme	glycodelin	1629:1638	arg1	antibody					1640:1647	a specific glycodelin antibody	1618:1647	a specific glycodelin antibody	1618:1647	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	11	57	theme	UEAI	1684:1687	arg1	abundant					1713:1720	abundant	1713:1720	abundant	1713:1720	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	11	57	theme	UEAI	1684:1687	arg1	glycodelin					1698:1707	the UEAI reactive glycodelin	1680:1707	the UEAI reactive glycodelin	1680:1707	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	7	58	theme	abundant	1127:1134	arg1	antennae					1148:1155	more abundant poly-LacNAc antennae	1122:1155	more abundant poly-LacNAc antennae	1122:1155	These included a lower level of sialylation and more abundant poly-LacNAc antennae, some of which are fucosylated.					
32205858	9	59	theme	cell	1455:1458	arg1	proliferation					1460:1472	peripheral blood mononuclear cell proliferation	1426:1472	peripheral blood mononuclear cell proliferation	1426:1472	Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation.					
32205858	4	60	theme	HEC-1B	610:615	arg1	cells					645:649	HEC-1B human endometrial carcinoma cells	610:649	HEC-1B human endometrial carcinoma cells (HEC-1B Gd)	610:661	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	4	60	theme	HEC-1B	610:615	arg1	Gd					659:660	HEC-1B Gd	652:660	HEC-1B Gd	652:660	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	11	61	theme	endometrial	1725:1735	arg1	carcinoma					1737:1745	endometrial carcinoma	1725:1745	endometrial carcinoma	1725:1745	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	4	62	theme	mass	666:669	arg1	spectrometry					671:682	mass spectrometry glycomics strategies	666:703	mass spectrometry glycomics strategies	666:703	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	2	63	theme	endometrial	324:334	arg1	cells					346:350	endometrial carcinoma cells	324:350	endometrial carcinoma cells	324:350	It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype.					
32205858	6	64	theme	glycodelin	1017:1026	arg1	glycodelin-A					1037:1048	glycodelin-A	1037:1048	glycodelin-A (GdA)	1037:1054	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	6	64	theme	glycodelin	1017:1026	arg1	isoform					1028:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	4	65	theme	glycomics	684:692	arg1	strategies					694:703	glycomics strategies	684:703	mass spectrometry glycomics strategies	666:703	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	5	66	theme	Gd	724:725	arg1	Glycans					706:712	Glycans	706:712	Glycans of HEC-1B Gd	706:725	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	5	67	theme	LacNAc	850:855	arg1	antennae					873:880	undecorated LacNAc (Galβ1-4GlcNAc) antennae	838:880	undecorated LacNAc (Galβ1-4GlcNAc) antennae	838:880	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	12	68	theme	normal	2054:2059	arg1	endometrium					2061:2071	normal endometrium	2054:2071	normal endometrium	2054:2071	In conclusion, we established a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin and showed that this specific glycodelin is exclusively expressed in cancer, not in normal endometrium.					
32205858	6	69	theme	decidualized	984:995	arg1	glycodelin-A					1037:1048	glycodelin-A	1037:1048	glycodelin-A (GdA)	1037:1054	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	6	69	theme	decidualized	984:995	arg1	isoform					1028:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	4	70	theme	HEC-1B	652:657	arg1	cells					645:649	HEC-1B human endometrial carcinoma cells	610:649	HEC-1B human endometrial carcinoma cells (HEC-1B Gd)	610:661	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	4	70	theme	HEC-1B	652:657	arg1	Gd					659:660	HEC-1B Gd	652:660	HEC-1B Gd	652:660	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	5	71	theme	Galβ1-4GlcNAc	858:870	arg1	antennae					873:880	undecorated LacNAc (Galβ1-4GlcNAc) antennae	838:880	undecorated LacNAc (Galβ1-4GlcNAc) antennae	838:880	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	6	72	theme	normal	971:976	arg1	glycodelin-A					1037:1048	glycodelin-A	1037:1048	glycodelin-A (GdA)	1037:1054	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	6	72	theme	normal	971:976	arg1	isoform					1028:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	normal human decidualized endometrium-derived glycodelin isoform	971:1034	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	8	73	theme	different	1235:1243	arg1	binding					1245:1251	different binding	1235:1251	different binding to these classes of glycodelin	1235:1282	This allowed us to select lectins that showed different binding to these classes of glycodelin.					
32205858	7	74	theme	lower	1091:1095	arg1	level					1097:1101	a lower level	1089:1101	a lower level	1089:1101	These included a lower level of sialylation and more abundant poly-LacNAc antennae, some of which are fucosylated.					
32205858	10	75	dep	in	1549:1550	arg1	situ					1552:1555	situ	1552:1555	situ	1552:1555	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	4	76	theme	carcinoma	635:643	arg1	cells					645:649	HEC-1B human endometrial carcinoma cells	610:649	HEC-1B human endometrial carcinoma cells (HEC-1B Gd)	610:661	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	4	76	theme	carcinoma	635:643	arg1	Gd					659:660	HEC-1B Gd	652:660	HEC-1B Gd	652:660	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	3	77	gly	glycosylation	426:438	arg1	carcinoma					493:501	endometrial carcinoma	481:501	endometrial carcinoma	481:501	Here we aimed to elucidate whether the glycosylation and function of glycodelin is altered in endometrial carcinoma as compared with a normal endometrium.					
32205858	3	77	gly	glycosylation	426:438	arg1	glycodelin					456:465	glycodelin	456:465	glycodelin	456:465	Here we aimed to elucidate whether the glycosylation and function of glycodelin is altered in endometrial carcinoma as compared with a normal endometrium.					
32205858	0	78	from	glycosylation	8:20	arg1	carcinoma					51:59	endometrial carcinoma	39:59	endometrial carcinoma	39:59	Altered glycosylation of glycodelin in endometrial carcinoma.					
32205858	13	79	gly	glycoproteins	2123:2135	arg1	glycoproteins					2123:2135	other glycoproteins	2117:2135	other glycoproteins	2117:2135	Similar methods can be used for studies of other glycoproteins.					
32205858	5	80	theme	typical	752:758	arg1	mixture					760:766	a typical mixture	750:766	a typical mixture	750:766	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	13	81	theme	other	2117:2121	arg1	glycoproteins					2123:2135	other glycoproteins	2117:2135	other glycoproteins	2117:2135	Similar methods can be used for studies of other glycoproteins.					
32205858	8	82	theme	glycodelin	1273:1282	arg1	classes					1262:1268	these classes	1256:1268	these classes of glycodelin	1256:1282	This allowed us to select lectins that showed different binding to these classes of glycodelin.					
32205858	10	83	theme	associated	1503:1512	arg1	glycodelin					1514:1523	cancer associated glycodelin	1496:1523	cancer associated glycodelin	1496:1523	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	3	84	theme	glycodelin	456:465	arg1	function					444:451	function	444:451	function	444:451	Here we aimed to elucidate whether the glycosylation and function of glycodelin is altered in endometrial carcinoma as compared with a normal endometrium.					
32205858	3	84	theme	glycodelin	456:465	arg1	glycosylation					426:438	glycosylation	426:438	glycosylation	426:438	Here we aimed to elucidate whether the glycosylation and function of glycodelin is altered in endometrial carcinoma as compared with a normal endometrium.					
32205858	11	85	from	abundant	1713:1720	arg1	tissue					1791:1796	normal endometrial tissue	1772:1796	normal endometrial tissue	1772:1796	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	11	85	from	abundant	1713:1720	arg1	carcinoma					1737:1745	endometrial carcinoma	1725:1745	endometrial carcinoma	1725:1745	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	3	86	dep	glycosylation	426:438	arg1	the					422:424	the	422:424	the	422:424	Here we aimed to elucidate whether the glycosylation and function of glycodelin is altered in endometrial carcinoma as compared with a normal endometrium.					
32205858	0	87	theme	glycodelin	25:34	arg1	glycosylation					8:20	Altered glycosylation	0:20	Altered glycosylation of glycodelin in endometrial carcinoma	0:59	Altered glycosylation of glycodelin in endometrial carcinoma.					
32205858	12	88	theme	endometrial	1926:1936	arg1	glycodelin					1959:1968	endometrial carcinoma-associated glycodelin	1926:1968	endometrial carcinoma-associated glycodelin	1926:1968	In conclusion, we established a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin and showed that this specific glycodelin is exclusively expressed in cancer, not in normal endometrium.					
32205858	4	89	theme	glycodelin	586:595	arg1	analysis					574:581	glycan structure analysis	557:581	glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd)	557:661	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	2	90	theme	reproduction	192:203	arg1	functions					213:221	several reproduction related functions	184:221	several reproduction related functions that are dependent on specific glycosylation	184:266	It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype.					
32205858	3	91	theme	endometrial	481:491	arg1	carcinoma					493:501	endometrial carcinoma	481:501	endometrial carcinoma	481:501	Here we aimed to elucidate whether the glycosylation and function of glycodelin is altered in endometrial carcinoma as compared with a normal endometrium.					
32205858	0	92	theme	endometrial	39:49	arg1	carcinoma					51:59	endometrial carcinoma	39:59	endometrial carcinoma	39:59	Altered glycosylation of glycodelin in endometrial carcinoma.					
32205858	9	93	theme	similar	1365:1371	arg1	activity					1384:1391	similar inhibitory activity	1365:1391	similar inhibitory activity	1365:1391	Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation.					
32205858	12	94	theme	glycodelin	1959:1968	arg1	detection					1913:1921	the detection	1909:1921	the detection of endometrial carcinoma-associated glycodelin	1909:1968	In conclusion, we established a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin and showed that this specific glycodelin is exclusively expressed in cancer, not in normal endometrium.					
32205858	5	95	theme	complex-type	797:808	arg1	N-glycans					810:818	complex-type N-glycans	797:818	complex-type N-glycans	797:818	Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Galβ1-4GlcNAc) antennae.					
32205858	4	96	theme	glycan	557:562	arg1	analysis					574:581	glycan structure analysis	557:581	glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd)	557:661	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	11	97	theme	normal	1772:1777	arg1	tissue					1791:1796	normal endometrial tissue	1772:1796	normal endometrial tissue	1772:1796	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	9	98	theme	trophoblast	1396:1406	arg1	invasion					1413:1420	trophoblast cell invasion	1396:1420	trophoblast cell invasion	1396:1420	Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation.					
32205858	12	99	theme	histochemical	1875:1887	arg1	method					1898:1903	a histochemical staining method	1873:1903	a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin	1873:1968	In conclusion, we established a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin and showed that this specific glycodelin is exclusively expressed in cancer, not in normal endometrium.					
32205858	6	100	dep	differences	900:910	arg1	compared					916:923	compared	916:923	compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA)	916:1054	However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found.					
32205858	10	101	theme	novel	1543:1547	arg1	method					1605:1610	a novel in situ proximity-ligation based histochemical staining method	1541:1610	a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin	1541:1663	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	2	102	theme	carcinoma	336:344	arg1	cells					346:350	endometrial carcinoma cells	324:350	endometrial carcinoma cells	324:350	It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype.					
32205858	4	103	dep	spectrometry	671:682	arg1	strategies					694:703	glycomics strategies	684:703	mass spectrometry glycomics strategies	666:703	We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies.					
32205858	10	104	theme	staining	1596:1603	arg1	method					1605:1610	a novel in situ proximity-ligation based histochemical staining method	1541:1610	a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin	1541:1663	For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin.					
32205858	11	105	theme	reactive	1689:1696	arg1	abundant					1713:1720	abundant	1713:1720	abundant	1713:1720	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	11	105	theme	reactive	1689:1696	arg1	glycodelin					1698:1707	the UEAI reactive glycodelin	1680:1707	the UEAI reactive glycodelin	1680:1707	We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed.					
32205858	7	106	gly	fucosylated	1176:1186	arg1	some					1158:1161	some	1158:1161	some	1158:1161	These included a lower level of sialylation and more abundant poly-LacNAc antennae, some of which are fucosylated.					
32205858	7	106	gly	fucosylated	1176:1186	arg1	fucosylated					1176:1186	fucosylated	1176:1186	fucosylated	1176:1186	These included a lower level of sialylation and more abundant poly-LacNAc antennae, some of which are fucosylated.					
32205858	7	106	gly	fucosylated	1176:1186	arg1	level					1097:1101	a lower level	1089:1101	a lower level	1089:1101	These included a lower level of sialylation and more abundant poly-LacNAc antennae, some of which are fucosylated.					
32205858	9	107	theme	peripheral	1426:1435	arg1	cell					1455:1458	peripheral blood mononuclear cell	1426:1458	peripheral blood mononuclear cell proliferation	1426:1472	Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation.					
33876795	8	0	theme	target	1630:1635	arg1	enzymes					1637:1643	the target enzymes	1626:1643	the target enzymes	1626:1643	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	3	1	theme	selective	563:571	arg1	production					573:582	selective production	563:582	selective production of signal glycans	563:600	Although selective production of signal glycans is important in determining the fate of glycoproteins, the branch-discrimination abilities of the α-1,2-mannosidases are not well understood.					
33876795	7	2	theme	solvent	1524:1530	arg1	polarity					1502:1509	the polarity	1498:1509	tuning the polarity of the mixed solvent	1491:1530	When synthesizing these oligosaccharides, highly stereoselective glycosylation was achieved with a high yield in each case by adding a weak base or tuning the polarity of the mixed solvent.					
33876795	4	3	theme	glycosidic	902:911	arg1	linkages					913:920	the adjacent inner glycosidic linkages	883:920	the adjacent inner glycosidic linkages	883:920	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	4	3	theme	glycosidic	902:911	arg1	different					926:934	different	926:934	different	926:934	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	1	4	gly	glycoproteins	218:230	arg1	glycoproteins					218:230	endoplasmic reticulum (ER) glycoproteins	191:230	endoplasmic reticulum (ER) glycoproteins	191:230	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	3	5	theme	glycans	594:600	arg1	production					573:582	selective production	563:582	selective production of signal glycans	563:600	Although selective production of signal glycans is important in determining the fate of glycoproteins, the branch-discrimination abilities of the α-1,2-mannosidases are not well understood.					
33876795	4	6	theme	structural	746:755	arg1	feature					757:763	A structural feature	744:763	A structural feature of the Man9GlcNAc2 glycan	744:789	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	9	7	theme	competitive	1795:1805	arg1	experiments					1807:1817	Various competitive experiments	1787:1817	Various competitive experiments	1787:1817	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	8	8	from	trisaccharides	1713:1726	arg1	order					1735:1739	the order	1731:1739	the order of A-branch > B-branch > C-branch ≈ D-branch	1731:1784	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	8	9	theme	Enzymatic	1533:1541	arg1	hydrolysis					1543:1552	Enzymatic hydrolysis	1533:1552	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes	1533:1643	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	3	10	gly	glycoproteins	642:654	arg1	glycoproteins					642:654	glycoproteins	642:654	glycoproteins	642:654	Although selective production of signal glycans is important in determining the fate of glycoproteins, the branch-discrimination abilities of the α-1,2-mannosidases are not well understood.					
33876795	3	11	theme	glycoproteins	642:654	arg1	fate					634:637	the fate	630:637	the fate of glycoproteins	630:654	Although selective production of signal glycans is important in determining the fate of glycoproteins, the branch-discrimination abilities of the α-1,2-mannosidases are not well understood.					
33876795	1	12	theme	Man9GlcNAc2	154:164	arg1	glycan					166:171	A tri-antennary Man9GlcNAc2 glycan	138:171	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins	138:230	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	1	12	theme	Man9GlcNAc2	154:164	arg1	secretion					260:268	a glycoprotein secretion or degradation signal	245:290	secretion	260:268	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	9	13	theme	first	1841:1845	arg1	time					1847:1850	the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER	1837:1927	the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER	1837:1927	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	8	14	theme	synthetic	1561:1569	arg1	trisaccharides					1571:1584	the synthetic trisaccharides	1557:1584	the synthetic trisaccharides	1557:1584	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	8	15	contain	had	1683:1685	arg2	specificity					1693:1703	clear specificity	1687:1703	clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch	1687:1784	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	8	15	contain	had	1683:1685	arg1	α-1,2-mannosidases					1664:1681	the ER α-1,2-mannosidases	1657:1681	the ER α-1,2-mannosidases	1657:1681	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	0	16	theme	exo-acting	78:87	arg1	α-1,2-mannosidases					89:106	exo-acting α-1,2-mannosidases	78:106	exo-acting α-1,2-mannosidases in the endoplasmic reticulum	78:135	Synthetic trisaccharides reveal discrimination of endo-glycosidic linkages by exo-acting α-1,2-mannosidases in the endoplasmic reticulum.					
33876795	9	17	with	α-1,2-mannosidase	1857:1873	arg1	specificity					1896:1906	inner glycoside specificity	1880:1906	inner glycoside specificity	1880:1906	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	6	18	theme	different	1100:1108	arg1	Manα1-2Manα1-4Man					1249:1265	Manα1-2Manα1-4Man	1249:1265	Manα1-2Manα1-4Man (unnatural D-branch)	1249:1286	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	18	theme	different	1100:1108	arg1	Manα1-2Manα1-3Man					1169:1185	Manα1-2Manα1-3Man	1169:1185	Manα1-2Manα1-3Man (natural B-branch)	1169:1204	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	18	theme	different	1100:1108	arg1	Manα1-2Manα1-6Man					1207:1223	Manα1-2Manα1-6Man	1207:1223	Manα1-2Manα1-6Man (natural C-branch)	1207:1242	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	18	theme	different	1100:1108	arg1	linkages					1121:1128	different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)]	1100:1287	different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)]	1100:1287	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	18	theme	different	1100:1108	arg1	[Manα1-2Manα1-2Man					1130:1147	[Manα1-2Manα1-2Man	1130:1147	[Manα1-2Manα1-2Man (natural A-branch)	1130:1166	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	2	19	theme	signal	538:543	arg1	glycans					545:551	these signal glycans	532:551	these signal glycans	532:551	Four α-1,2-mannosidases-ER mannosidase I, ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1), EDEM2, and EDEM3-are involved in the production of these signal glycans.					
33876795	8	20	theme	mouse	1591:1595	arg1	liver					1597:1601	mouse liver	1591:1601	a mouse liver ER fraction containing the target enzymes	1589:1643	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	4	21	theme	Man9GlcNAc2	772:782	arg1	glycan					784:789	the Man9GlcNAc2 glycan	768:789	the Man9GlcNAc2 glycan	768:789	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	1	22	theme	endoplasmic	191:201	arg1	ER					214:215	ER	214:215	ER	214:215	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	1	22	theme	endoplasmic	191:201	arg1	reticulum					203:211	endoplasmic reticulum	191:211	endoplasmic reticulum (ER) glycoproteins	191:230	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	9	23	theme	glycoside	1886:1894	arg1	specificity					1896:1906	inner glycoside specificity	1880:1906	inner glycoside specificity	1880:1906	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	8	24	theme	ER	1603:1604	arg1	fraction					1606:1613	a mouse liver ER fraction	1589:1613	a mouse liver ER fraction containing the target enzymes	1589:1643	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	0	25	theme	endoplasmic	115:125	arg1	reticulum					127:135	the endoplasmic reticulum	111:135	the endoplasmic reticulum	111:135	Synthetic trisaccharides reveal discrimination of endo-glycosidic linkages by exo-acting α-1,2-mannosidases in the endoplasmic reticulum.					
33876795	5	26	theme	different	1047:1055	arg1	glycosides					1063:1072	different inner glycosides	1047:1072	different inner glycosides	1047:1072	In this study, we examined whether the α-1,2-mannosidases showed branch specificity by discriminating between different inner glycosides.					
33876795	1	27	from	glycan	166:171	arg1	surface					180:186	the surface	176:186	the surface of endoplasmic reticulum (ER) glycoproteins	176:230	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	6	28	dep	linkages	1121:1128	arg1	C-branch					1234:1241	natural C-branch	1226:1241	natural C-branch	1226:1241	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	28	dep	linkages	1121:1128	arg1	A-branch					1158:1165	natural A-branch	1150:1165	natural A-branch	1150:1165	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	28	dep	linkages	1121:1128	arg1	D-branch					1278:1285	unnatural D-branch	1268:1285	unnatural D-branch	1268:1285	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	28	dep	linkages	1121:1128	arg1	B-branch					1196:1203	natural B-branch	1188:1203	natural B-branch	1188:1203	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	28	dep	linkages	1121:1128	arg1	Manα1-2Manα1-6Man					1207:1223	Manα1-2Manα1-6Man	1207:1223	Manα1-2Manα1-6Man (natural C-branch)	1207:1242	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	28	dep	linkages	1121:1128	arg1	linkages					1121:1128	different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)]	1100:1287	different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)]	1100:1287	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	28	dep	linkages	1121:1128	arg1	[Manα1-2Manα1-2Man					1130:1147	[Manα1-2Manα1-2Man	1130:1147	[Manα1-2Manα1-2Man (natural A-branch)	1130:1166	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	28	dep	linkages	1121:1128	arg1	Manα1-2Manα1-4Man					1249:1265	Manα1-2Manα1-4Man	1249:1265	Manα1-2Manα1-4Man (unnatural D-branch)	1249:1286	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	28	dep	linkages	1121:1128	arg1	Manα1-2Manα1-3Man					1169:1185	Manα1-2Manα1-3Man	1169:1185	Manα1-2Manα1-3Man (natural B-branch)	1169:1204	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	4	29	theme	glycosidic	812:821	arg1	linkages					823:830	all terminal glycosidic linkages	799:830	all terminal glycosidic linkages of the three branches	799:852	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	0	30	theme	Synthetic	0:8	arg1	trisaccharides					10:23	Synthetic trisaccharides	0:23	Synthetic trisaccharides	0:23	Synthetic trisaccharides reveal discrimination of endo-glycosidic linkages by exo-acting α-1,2-mannosidases in the endoplasmic reticulum.					
33876795	7	31	theme	high	1442:1445	arg1	yield					1447:1451	a high yield	1440:1451	a high yield in each case	1440:1464	When synthesizing these oligosaccharides, highly stereoselective glycosylation was achieved with a high yield in each case by adding a weak base or tuning the polarity of the mixed solvent.					
33876795	1	32	theme	terminal	329:336	arg1	residue					352:358	the terminal α-1,2-mannose residue	325:358	the terminal α-1,2-mannose residue of each branch	325:373	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	1	32	theme	terminal	329:336	arg1	branch					368:373	each branch	363:373	each branch	363:373	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	1	33	theme	residue	352:358	arg1	cleavage					313:320	regioselective cleavage	298:320	regioselective cleavage of the terminal α-1,2-mannose residue of each branch	298:373	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	10	34	theme	exo-acting	1956:1965	arg1	α-1,2-mannosidases					1970:1987	exo-acting ER α-1,2-mannosidases	1956:1987	exo-acting ER α-1,2-mannosidases	1956:1987	Our findings suggest that exo-acting ER α-1,2-mannosidases can discriminate between endo-glycosidic linkages.					
33876795	4	35	theme	adjacent	887:894	arg1	linkages					913:920	the adjacent inner glycosidic linkages	883:920	the adjacent inner glycosidic linkages	883:920	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	4	35	theme	adjacent	887:894	arg1	different					926:934	different	926:934	different	926:934	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	2	36	theme	mannosidase	403:413	arg1	I					415:415	Four α-1,2-mannosidases-ER mannosidase I	376:415	Four α-1,2-mannosidases-ER mannosidase I	376:415	Four α-1,2-mannosidases-ER mannosidase I, ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1), EDEM2, and EDEM3-are involved in the production of these signal glycans.					
33876795	0	37	theme	linkages	66:73	arg1	discrimination					32:45	discrimination	32:45	discrimination of endo-glycosidic linkages by exo-acting α-1,2-mannosidases in the endoplasmic reticulum	32:135	Synthetic trisaccharides reveal discrimination of endo-glycosidic linkages by exo-acting α-1,2-mannosidases in the endoplasmic reticulum.					
33876795	7	38	theme	stereoselective	1392:1406	arg1	glycosylation					1408:1420	highly stereoselective glycosylation	1385:1420	highly stereoselective glycosylation	1385:1420	When synthesizing these oligosaccharides, highly stereoselective glycosylation was achieved with a high yield in each case by adding a weak base or tuning the polarity of the mixed solvent.					
33876795	8	39	theme	clear	1687:1691	arg1	specificity					1693:1703	clear specificity	1687:1703	clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch	1687:1784	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	10	40	theme	endo-glycosidic	2014:2028	arg1	linkages					2030:2037	endo-glycosidic linkages	2014:2037	endo-glycosidic linkages	2014:2037	Our findings suggest that exo-acting ER α-1,2-mannosidases can discriminate between endo-glycosidic linkages.					
33876795	1	41	theme	glycoprotein	247:258	arg1	glycan					166:171	A tri-antennary Man9GlcNAc2 glycan	138:171	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins	138:230	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	1	41	theme	glycoprotein	247:258	arg1	secretion					260:268	a glycoprotein secretion or degradation signal	245:290	secretion	260:268	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	6	42	theme	unnatural	1268:1276	arg1	Manα1-2Manα1-4Man					1249:1265	Manα1-2Manα1-4Man	1249:1265	Manα1-2Manα1-4Man (unnatural D-branch)	1249:1286	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	42	theme	unnatural	1268:1276	arg1	D-branch					1278:1285	unnatural D-branch	1268:1285	unnatural D-branch	1268:1285	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	2	43	theme	degradation-enhancing	421:441	arg1	protein					462:468	ER degradation-enhancing α-mannosidase-like protein 1	418:470	ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1)	418:478	Four α-1,2-mannosidases-ER mannosidase I, ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1), EDEM2, and EDEM3-are involved in the production of these signal glycans.					
33876795	2	43	theme	degradation-enhancing	421:441	arg1	EDEM1					473:477	EDEM1	473:477	EDEM1	473:477	Four α-1,2-mannosidases-ER mannosidase I, ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1), EDEM2, and EDEM3-are involved in the production of these signal glycans.					
33876795	6	44	used	used	1310:1313	arg2	trisaccharides					1080:1093	Four trisaccharides	1075:1093	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)]	1075:1287	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	7	45	from	yield	1447:1451	arg1	case					1461:1464	each case	1456:1464	each case	1456:1464	When synthesizing these oligosaccharides, highly stereoselective glycosylation was achieved with a high yield in each case by adding a weak base or tuning the polarity of the mixed solvent.					
33876795	1	46	theme	regioselective	298:311	arg1	cleavage					313:320	regioselective cleavage	298:320	regioselective cleavage of the terminal α-1,2-mannose residue of each branch	298:373	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	9	47	from	ER	1926:1927	arg1	present					1911:1917	present	1911:1917	present	1911:1917	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	3	48	theme	signal	587:592	arg1	glycans					594:600	signal glycans	587:600	signal glycans	587:600	Although selective production of signal glycans is important in determining the fate of glycoproteins, the branch-discrimination abilities of the α-1,2-mannosidases are not well understood.					
33876795	1	49	theme	reticulum	203:211	arg1	glycoproteins					218:230	endoplasmic reticulum (ER) glycoproteins	191:230	endoplasmic reticulum (ER) glycoproteins	191:230	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	4	50	theme	inner	896:900	arg1	linkages					913:920	the adjacent inner glycosidic linkages	883:920	the adjacent inner glycosidic linkages	883:920	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	4	50	theme	inner	896:900	arg1	different					926:934	different	926:934	different	926:934	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	7	51	theme	mixed	1518:1522	arg1	solvent					1524:1530	the mixed solvent	1514:1530	the mixed solvent	1514:1530	When synthesizing these oligosaccharides, highly stereoselective glycosylation was achieved with a high yield in each case by adding a weak base or tuning the polarity of the mixed solvent.					
33876795	8	52	contain	containing	1615:1624	arg2	enzymes					1637:1643	the target enzymes	1626:1643	the target enzymes	1626:1643	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	8	52	contain	containing	1615:1624	arg1	fraction					1606:1613	a mouse liver ER fraction	1589:1613	a mouse liver ER fraction containing the target enzymes	1589:1643	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	9	53	theme	Various	1787:1793	arg1	experiments					1807:1817	Various competitive experiments	1787:1817	Various competitive experiments	1787:1817	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	9	54	attach	present	1911:1917	arg1	ER					1926:1927	the ER	1922:1927	the ER	1922:1927	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	9	54	attach	present	1911:1917	arg2	α-1,2-mannosidase					1857:1873	α-1,2-mannosidase	1857:1873	α-1,2-mannosidase with inner glycoside specificity	1857:1906	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	1	55	theme	glycoproteins	218:230	arg1	surface					180:186	the surface	176:186	the surface of endoplasmic reticulum (ER) glycoproteins	176:230	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	0	56	from	α-1,2-mannosidases	89:106	arg1	reticulum					127:135	the endoplasmic reticulum	111:135	the endoplasmic reticulum	111:135	Synthetic trisaccharides reveal discrimination of endo-glycosidic linkages by exo-acting α-1,2-mannosidases in the endoplasmic reticulum.					
33876795	1	57	theme	tri-antennary	140:152	arg1	glycan					166:171	A tri-antennary Man9GlcNAc2 glycan	138:171	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins	138:230	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	1	57	theme	tri-antennary	140:152	arg1	secretion					260:268	a glycoprotein secretion or degradation signal	245:290	secretion	260:268	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	8	58	theme	trisaccharides	1571:1584	arg1	hydrolysis					1543:1552	Enzymatic hydrolysis	1533:1552	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes	1533:1643	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	7	59	theme	weak	1478:1481	arg1	base					1483:1486	a weak base	1476:1486	a weak base	1476:1486	When synthesizing these oligosaccharides, highly stereoselective glycosylation was achieved with a high yield in each case by adding a weak base or tuning the polarity of the mixed solvent.					
33876795	4	60	theme	glycan	784:789	arg1	feature					757:763	A structural feature	744:763	A structural feature of the Man9GlcNAc2 glycan	744:789	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	3	61	theme	branch-discrimination	661:681	arg1	abilities					683:691	the branch-discrimination abilities	657:691	the branch-discrimination abilities of the α-1,2-mannosidases	657:717	Although selective production of signal glycans is important in determining the fate of glycoproteins, the branch-discrimination abilities of the α-1,2-mannosidases are not well understood.					
33876795	7	62	dep	tuning	1491:1496	arg1	polarity					1502:1509	the polarity	1498:1509	tuning the polarity of the mixed solvent	1491:1530	When synthesizing these oligosaccharides, highly stereoselective glycosylation was achieved with a high yield in each case by adding a weak base or tuning the polarity of the mixed solvent.					
33876795	6	63	theme	glycosidic	1110:1119	arg1	Manα1-2Manα1-4Man					1249:1265	Manα1-2Manα1-4Man	1249:1265	Manα1-2Manα1-4Man (unnatural D-branch)	1249:1286	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	63	theme	glycosidic	1110:1119	arg1	Manα1-2Manα1-3Man					1169:1185	Manα1-2Manα1-3Man	1169:1185	Manα1-2Manα1-3Man (natural B-branch)	1169:1204	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	63	theme	glycosidic	1110:1119	arg1	Manα1-2Manα1-6Man					1207:1223	Manα1-2Manα1-6Man	1207:1223	Manα1-2Manα1-6Man (natural C-branch)	1207:1242	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	63	theme	glycosidic	1110:1119	arg1	linkages					1121:1128	different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)]	1100:1287	different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)]	1100:1287	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	63	theme	glycosidic	1110:1119	arg1	[Manα1-2Manα1-2Man					1130:1147	[Manα1-2Manα1-2Man	1130:1147	[Manα1-2Manα1-2Man (natural A-branch)	1130:1166	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	2	64	theme	glycans	545:551	arg1	production					518:527	the production	514:527	the production of these signal glycans	514:551	Four α-1,2-mannosidases-ER mannosidase I, ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1), EDEM2, and EDEM3-are involved in the production of these signal glycans.					
33876795	8	65	theme	liver	1597:1601	arg1	fraction					1606:1613	a mouse liver ER fraction	1589:1613	a mouse liver ER fraction containing the target enzymes	1589:1643	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	6	66	with	trisaccharides	1080:1093	arg1	Manα1-2Manα1-4Man					1249:1265	Manα1-2Manα1-4Man	1249:1265	Manα1-2Manα1-4Man (unnatural D-branch)	1249:1286	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	66	with	trisaccharides	1080:1093	arg1	Manα1-2Manα1-3Man					1169:1185	Manα1-2Manα1-3Man	1169:1185	Manα1-2Manα1-3Man (natural B-branch)	1169:1204	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	66	with	trisaccharides	1080:1093	arg1	Manα1-2Manα1-6Man					1207:1223	Manα1-2Manα1-6Man	1207:1223	Manα1-2Manα1-6Man (natural C-branch)	1207:1242	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	66	with	trisaccharides	1080:1093	arg1	linkages					1121:1128	different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)]	1100:1287	different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)]	1100:1287	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	66	with	trisaccharides	1080:1093	arg1	[Manα1-2Manα1-2Man					1130:1147	[Manα1-2Manα1-2Man	1130:1147	[Manα1-2Manα1-2Man (natural A-branch)	1130:1166	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	9	67	theme	inner	1880:1884	arg1	specificity					1896:1906	inner glycoside specificity	1880:1906	inner glycoside specificity	1880:1906	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	1	68	gly	glycoprotein	247:258	arg1	glycoprotein					247:258	a glycoprotein secretion or degradation signal	245:290	glycoprotein	247:258	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	5	69	theme	inner	1057:1061	arg1	glycosides					1063:1072	different inner glycosides	1047:1072	different inner glycosides	1047:1072	In this study, we examined whether the α-1,2-mannosidases showed branch specificity by discriminating between different inner glycosides.					
33876795	6	70	theme	natural	1188:1194	arg1	Manα1-2Manα1-3Man					1169:1185	Manα1-2Manα1-3Man	1169:1185	Manα1-2Manα1-3Man (natural B-branch)	1169:1204	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	70	theme	natural	1188:1194	arg1	B-branch					1196:1203	natural B-branch	1188:1203	natural B-branch	1188:1203	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	4	71	theme	branches	845:852	arg1	linkages					823:830	all terminal glycosidic linkages	799:830	all terminal glycosidic linkages of the three branches	799:852	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	5	72	theme	branch	1002:1007	arg1	specificity					1009:1019	branch specificity	1002:1019	branch specificity	1002:1019	In this study, we examined whether the α-1,2-mannosidases showed branch specificity by discriminating between different inner glycosides.					
33876795	4	73	theme	terminal	803:810	arg1	linkages					823:830	all terminal glycosidic linkages	799:830	all terminal glycosidic linkages of the three branches	799:852	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	2	74	theme	α-mannosidase-like	443:460	arg1	protein					462:468	ER degradation-enhancing α-mannosidase-like protein 1	418:470	ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1)	418:478	Four α-1,2-mannosidases-ER mannosidase I, ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1), EDEM2, and EDEM3-are involved in the production of these signal glycans.					
33876795	2	74	theme	α-mannosidase-like	443:460	arg1	EDEM1					473:477	EDEM1	473:477	EDEM1	473:477	Four α-1,2-mannosidases-ER mannosidase I, ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1), EDEM2, and EDEM3-are involved in the production of these signal glycans.					
33876795	9	75	located	present	1911:1917	arg1	ER					1926:1927	the ER	1922:1927	the ER	1922:1927	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	9	75	located	present	1911:1917	arg2	α-1,2-mannosidase					1857:1873	α-1,2-mannosidase	1857:1873	α-1,2-mannosidase with inner glycoside specificity	1857:1906	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	8	76	theme	ER	1661:1662	arg1	α-1,2-mannosidases					1664:1681	the ER α-1,2-mannosidases	1657:1681	the ER α-1,2-mannosidases	1657:1681	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	0	77	theme	endo-glycosidic	50:64	arg1	linkages					66:73	endo-glycosidic linkages	50:73	endo-glycosidic linkages	50:73	Synthetic trisaccharides reveal discrimination of endo-glycosidic linkages by exo-acting α-1,2-mannosidases in the endoplasmic reticulum.					
33876795	1	78	theme	α-1,2-mannose	338:350	arg1	residue					352:358	the terminal α-1,2-mannose residue	325:358	the terminal α-1,2-mannose residue of each branch	325:373	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	1	78	theme	α-1,2-mannose	338:350	arg1	branch					368:373	each branch	363:373	each branch	363:373	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	9	79	from	present	1911:1917	arg1	ER					1926:1927	the ER	1922:1927	the ER	1922:1927	Various competitive experiments have revealed for the first time that α-1,2-mannosidase with inner glycoside specificity is present in the ER.					
33876795	6	80	theme	natural	1150:1156	arg1	A-branch					1158:1165	natural A-branch	1150:1165	natural A-branch	1150:1165	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	80	theme	natural	1150:1156	arg1	[Manα1-2Manα1-2Man					1130:1147	[Manα1-2Manα1-2Man	1130:1147	[Manα1-2Manα1-2Man (natural A-branch)	1130:1166	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	3	81	theme	α-1,2-mannosidases	700:717	arg1	abilities					683:691	the branch-discrimination abilities	657:691	the branch-discrimination abilities of the α-1,2-mannosidases	657:717	Although selective production of signal glycans is important in determining the fate of glycoproteins, the branch-discrimination abilities of the α-1,2-mannosidases are not well understood.					
33876795	2	82	theme	α-1,2-mannosidases-ER	381:401	arg1	I					415:415	Four α-1,2-mannosidases-ER mannosidase I	376:415	Four α-1,2-mannosidases-ER mannosidase I	376:415	Four α-1,2-mannosidases-ER mannosidase I, ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1), EDEM2, and EDEM3-are involved in the production of these signal glycans.					
33876795	1	83	theme	branch	368:373	arg1	residue					352:358	the terminal α-1,2-mannose residue	325:358	the terminal α-1,2-mannose residue of each branch	325:373	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	1	83	theme	branch	368:373	arg1	branch					368:373	each branch	363:373	each branch	363:373	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
33876795	6	84	theme	natural	1226:1232	arg1	Manα1-2Manα1-6Man					1207:1223	Manα1-2Manα1-6Man	1207:1223	Manα1-2Manα1-6Man (natural C-branch)	1207:1242	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	6	84	theme	natural	1226:1232	arg1	C-branch					1234:1241	natural C-branch	1226:1241	natural C-branch	1226:1241	Four trisaccharides with different glycosidic linkages [Manα1-2Manα1-2Man (natural A-branch), Manα1-2Manα1-3Man (natural B-branch), Manα1-2Manα1-6Man (natural C-branch), and Manα1-2Manα1-4Man (unnatural D-branch)] were synthesized and used to evaluate the hypothesis.					
33876795	10	85	theme	ER	1967:1968	arg1	α-1,2-mannosidases					1970:1987	exo-acting ER α-1,2-mannosidases	1956:1987	exo-acting ER α-1,2-mannosidases	1956:1987	Our findings suggest that exo-acting ER α-1,2-mannosidases can discriminate between endo-glycosidic linkages.					
33876795	4	86	theme	α-1,2	865:869	arg1	type					871:874	the α-1,2 type	861:874	the α-1,2 type	861:874	A structural feature of the Man9GlcNAc2 glycan is that all terminal glycosidic linkages of the three branches are of the α-1,2 type, while the adjacent inner glycosidic linkages are different.					
33876795	2	87	theme	ER	418:419	arg1	protein					462:468	ER degradation-enhancing α-mannosidase-like protein 1	418:470	ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1)	418:478	Four α-1,2-mannosidases-ER mannosidase I, ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1), EDEM2, and EDEM3-are involved in the production of these signal glycans.					
33876795	2	87	theme	ER	418:419	arg1	EDEM1					473:477	EDEM1	473:477	EDEM1	473:477	Four α-1,2-mannosidases-ER mannosidase I, ER degradation-enhancing α-mannosidase-like protein 1 (EDEM1), EDEM2, and EDEM3-are involved in the production of these signal glycans.					
33876795	8	88	theme	D-branch	1777:1784	arg1	order					1735:1739	the order	1731:1739	the order of A-branch > B-branch > C-branch ≈ D-branch	1731:1784	Enzymatic hydrolysis of the synthetic trisaccharides by a mouse liver ER fraction containing the target enzymes showed that the ER α-1,2-mannosidases had clear specificity for the trisaccharides in the order of A-branch > B-branch > C-branch ≈ D-branch.					
33876795	1	89	theme	degradation	273:283	arg1	signal					285:290	a glycoprotein secretion or degradation signal	245:290	signal	285:290	A tri-antennary Man9GlcNAc2 glycan on the surface of endoplasmic reticulum (ER) glycoproteins functions as a glycoprotein secretion or degradation signal after regioselective cleavage of the terminal α-1,2-mannose residue of each branch.					
32617930	5	0	theme	hemicelluloses	857:870	arg1	presence					837:844	the presence	833:844	the presence of desired hemicelluloses and/or pectins	833:885	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	5	1	theme	characterized.The	716:732	arg1	method					734:739	characterized.The method	716:739	characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins	716:885	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	7	2	theme	intermediate	1166:1177	arg1	drying					1179:1184	intermediate drying	1166:1184	intermediate drying	1166:1184	Fermentation results in a floating mat or pellicle of cellulose-based composite that can be recovered, washed, and then studied under hydrated conditions without any need for intermediate drying.					
32617930	5	3	theme	composites	655:664	arg1	nature					641:646	The macroscopic nature	625:646	The macroscopic nature of the composites	625:664	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	6	4	theme	composites	979:988	arg1	construction					926:937	construction	926:937	construction of hemicellulose- and pectin-containing composites	926:988	Different conditions are required for construction of hemicellulose- and pectin-containing composites.					
32617930	4	5	theme	hemicellulose	550:562	arg1	structures					593:602	defined hemicellulose and/or pectin polysaccharide structures	542:602	defined hemicellulose and/or pectin polysaccharide structures	542:602	By studying structure/property relationships of cellulose composites, the effects of defined hemicellulose and/or pectin polysaccharide structures can be investigated.					
32617930	2	6	dep	K.	215:216	arg1	xylinus					218:224	K. xylinus	215:224	K. xylinus	215:224	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	4	7	theme	composites	515:524	arg1	relationships					488:500	structure/property relationships	469:500	structure/property relationships of cellulose composites	469:524	By studying structure/property relationships of cellulose composites, the effects of defined hemicellulose and/or pectin polysaccharide structures can be investigated.					
32617930	5	8	theme	mechanical	688:697	arg1	properties					699:708	composite mechanical properties	678:708	composite mechanical properties	678:708	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	5	9	theme	composite	678:686	arg1	properties					699:708	composite mechanical properties	678:708	composite mechanical properties	678:708	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	5	10	dep	composites	771:780	arg1	involves					782:789	involves	782:789	involves reviving	782:798	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	5	11	theme	pectins	879:885	arg1	presence					837:844	the presence	833:844	the presence of desired hemicelluloses and/or pectins	833:885	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	5	12	theme	macroscopic	629:639	arg1	nature					641:646	The macroscopic nature	625:646	The macroscopic nature of the composites	625:664	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	5	13	theme	desired	849:855	arg1	hemicelluloses					857:870	desired hemicelluloses	849:870	desired hemicelluloses	849:870	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	6	14	theme	Different	888:896	arg1	conditions					898:907	Different conditions	888:907	Different conditions	888:907	Different conditions are required for construction of hemicellulose- and pectin-containing composites.					
32617930	1	15	theme	Komagataeibacter	109:124	arg1	xylinus					126:132	Komagataeibacter xylinus	109:132	Komagataeibacter xylinus	109:132	Komagataeibacter xylinus synthesizes cellulose in an analogous fashion to plants.					
32617930	3	16	theme	walls	450:454	arg1	structure					410:418	structure	410:418	structure	410:418	These serve as general models for the assembly, structure, and properties of plant cell walls.					
32617930	3	16	theme	walls	450:454	arg1	properties					425:434	properties	425:434	properties	425:434	These serve as general models for the assembly, structure, and properties of plant cell walls.					
32617930	3	16	theme	walls	450:454	arg1	assembly					400:407	assembly	400:407	assembly	400:407	These serve as general models for the assembly, structure, and properties of plant cell walls.					
32617930	7	17	theme	composite	1061:1069	arg1	pellicle					1033:1040	a floating mat or pellicle	1015:1040	pellicle	1033:1040	Fermentation results in a floating mat or pellicle of cellulose-based composite that can be recovered, washed, and then studied under hydrated conditions without any need for intermediate drying.					
32617930	7	17	theme	composite	1061:1069	arg1	mat					1026:1028	a floating mat or pellicle	1015:1040	mat	1026:1028	Fermentation results in a floating mat or pellicle of cellulose-based composite that can be recovered, washed, and then studied under hydrated conditions without any need for intermediate drying.					
32617930	6	18	theme	pectin-containing	961:977	arg1	composites					979:988	hemicellulose- and pectin-containing composites	942:988	hemicellulose- and pectin-containing composites	942:988	Different conditions are required for construction of hemicellulose- and pectin-containing composites.					
32617930	4	19	theme	structure/property	469:486	arg1	relationships					488:500	structure/property relationships	469:500	structure/property relationships of cellulose composites	469:524	By studying structure/property relationships of cellulose composites, the effects of defined hemicellulose and/or pectin polysaccharide structures can be investigated.					
32617930	0	20	theme	Composites	29:38	arg1	Formation					0:8	Formation	0:8	Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Komagataeibacter Fermentation.	0:107	Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Komagataeibacter Fermentation.					
32617930	0	21	theme	Cellulose-Based	13:27	arg1	Composites					29:38	Cellulose-Based Composites	13:38	Cellulose-Based Composites	13:38	Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Komagataeibacter Fermentation.					
32617930	2	22	from	families	309:316	arg1	polysaccharides					256:270	cell wall polysaccharides	246:270	cell wall polysaccharides from the hemicellulose and/or pectin families	246:316	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	3	23	theme	plant	439:443	arg1	walls					450:454	plant cell walls	439:454	plant cell walls	439:454	These serve as general models for the assembly, structure, and properties of plant cell walls.					
32617930	2	24	theme	K.	215:216	arg1	fermentation					199:210	fermentation	199:210	fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families	199:316	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	2	25	contain	containing	235:244	arg1	media					229:233	media	229:233	media containing cell wall polysaccharides from the hemicellulose and/or pectin families	229:316	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	2	25	contain	containing	235:244	arg2	polysaccharides					256:270	cell wall polysaccharides	246:270	cell wall polysaccharides from the hemicellulose and/or pectin families	246:316	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	3	26	theme	cell	445:448	arg1	walls					450:454	plant cell walls	439:454	plant cell walls	439:454	These serve as general models for the assembly, structure, and properties of plant cell walls.					
32617930	4	27	theme	polysaccharide	578:591	arg1	structures					593:602	defined hemicellulose and/or pectin polysaccharide structures	542:602	defined hemicellulose and/or pectin polysaccharide structures	542:602	By studying structure/property relationships of cellulose composites, the effects of defined hemicellulose and/or pectin polysaccharide structures can be investigated.					
32617930	5	28	theme	K.	819:820	arg1	xylinus					822:828	K. xylinus	819:828	K. xylinus	819:828	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	4	29	theme	cellulose	505:513	arg1	composites					515:524	cellulose composites	505:524	cellulose composites	505:524	By studying structure/property relationships of cellulose composites, the effects of defined hemicellulose and/or pectin polysaccharide structures can be investigated.					
32617930	4	30	theme	pectin	571:576	arg1	structures					593:602	defined hemicellulose and/or pectin polysaccharide structures	542:602	defined hemicellulose and/or pectin polysaccharide structures	542:602	By studying structure/property relationships of cellulose composites, the effects of defined hemicellulose and/or pectin polysaccharide structures can be investigated.					
32617930	5	31	theme	cellulose-based	755:769	arg1	composites					771:780	cellulose-based composites	755:780	cellulose-based composites involves reviving	755:798	The macroscopic nature of the composites also allows composite mechanical properties to be characterized.The method for producing cellulose-based composites involves reviving and then culturing K. xylinus in the presence of desired hemicelluloses and/or pectins.					
32617930	2	32	from	fermentation	199:210	arg1	media					229:233	media	229:233	media containing cell wall polysaccharides from the hemicellulose and/or pectin families	229:316	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	2	33	theme	cell	246:249	arg1	polysaccharides					256:270	cell wall polysaccharides	246:270	cell wall polysaccharides from the hemicellulose and/or pectin families	246:316	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	2	34	theme	wall	251:254	arg1	polysaccharides					256:270	cell wall polysaccharides	246:270	cell wall polysaccharides from the hemicellulose and/or pectin families	246:316	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	7	35	theme	hydrated	1125:1132	arg1	conditions					1134:1143	hydrated conditions	1125:1143	hydrated conditions	1125:1143	Fermentation results in a floating mat or pellicle of cellulose-based composite that can be recovered, washed, and then studied under hydrated conditions without any need for intermediate drying.					
32617930	4	36	theme	structures	593:602	arg1	effects					531:537	the effects	527:537	the effects of defined hemicellulose and/or pectin polysaccharide structures	527:602	By studying structure/property relationships of cellulose composites, the effects of defined hemicellulose and/or pectin polysaccharide structures can be investigated.					
32617930	0	37	with	Formation	0:8	arg1	Hemicelluloses					45:58	Hemicelluloses	45:58	Hemicelluloses	45:58	Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Komagataeibacter Fermentation.					
32617930	0	37	with	Formation	0:8	arg1	Pectins					64:70	Pectins	64:70	Pectins	64:70	Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Komagataeibacter Fermentation.					
32617930	1	38	theme	analogous	162:170	arg1	fashion					172:178	an analogous fashion	159:178	an analogous fashion to plants	159:188	Komagataeibacter xylinus synthesizes cellulose in an analogous fashion to plants.					
32617930	7	39	theme	cellulose-based	1045:1059	arg1	composite					1061:1069	cellulose-based composite	1045:1069	cellulose-based composite that can be recovered, washed, and then studied under hydrated conditions without any need for intermediate drying	1045:1184	Fermentation results in a floating mat or pellicle of cellulose-based composite that can be recovered, washed, and then studied under hydrated conditions without any need for intermediate drying.					
32617930	2	40	with	composites	319:328	arg1	cellulose					335:343	cellulose	335:343	cellulose	335:343	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	3	41	dep	assembly	400:407	arg1	the					396:398	the	396:398	the	396:398	These serve as general models for the assembly, structure, and properties of plant cell walls.					
32617930	6	42	theme	hemicellulose-	942:955	arg1	composites					979:988	hemicellulose- and pectin-containing composites	942:988	hemicellulose- and pectin-containing composites	942:988	Different conditions are required for construction of hemicellulose- and pectin-containing composites.					
32617930	2	43	theme	pectin	302:307	arg1	families					309:316	the hemicellulose and/or pectin families	277:316	families	309:316	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	0	44	theme	Komagataeibacter	78:93	arg1	Fermentation					95:106	Komagataeibacter Fermentation	78:106	Komagataeibacter Fermentation	78:106	Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Komagataeibacter Fermentation.					
32617930	3	45	theme	general	377:383	arg1	These					362:366	These	362:366	These	362:366	These serve as general models for the assembly, structure, and properties of plant cell walls.					
32617930	3	45	theme	general	377:383	arg1	models					385:390	general models	377:390	general models for the assembly, structure, and properties of plant cell walls	377:454	These serve as general models for the assembly, structure, and properties of plant cell walls.					
32617930	4	46	theme	defined	542:548	arg1	structures					593:602	defined hemicellulose and/or pectin polysaccharide structures	542:602	defined hemicellulose and/or pectin polysaccharide structures	542:602	By studying structure/property relationships of cellulose composites, the effects of defined hemicellulose and/or pectin polysaccharide structures can be investigated.					
32617930	2	47	theme	hemicellulose	281:293	arg1	families					309:316	the hemicellulose and/or pectin families	277:316	families	309:316	Through fermentation of K. xylinus in media containing cell wall polysaccharides from the hemicellulose and/or pectin families, composites with cellulose can be produced.					
32617930	7	48	theme	floating	1017:1024	arg1	mat					1026:1028	a floating mat or pellicle	1015:1040	mat	1026:1028	Fermentation results in a floating mat or pellicle of cellulose-based composite that can be recovered, washed, and then studied under hydrated conditions without any need for intermediate drying.					
34019602	1	0	theme	host-cell	142:150	arg1	ACE2					162:165	ACE2	162:165	ACE2	162:165	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	1	0	theme	host-cell	142:150	arg1	receptor					152:159	the host-cell receptor	138:159	the host-cell receptor	138:159	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	1	1	theme	receptor	152:159	arg1	glycans					127:133	the glycans	123:133	the glycans of the host-cell receptor, ACE2,	123:166	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	4	2	theme	site	633:636	arg1	absence					603:609	The absence	599:609	The absence of this glycosylation site on SARS-CoV-2 RBD	599:654	The absence of this glycosylation site on SARS-CoV-2 RBD may enhance its binding with ACE2.					
34019602	2	3	theme	ACE2	275:278	arg1	N322					290:293	the ACE2 glycan at N322	271:293	the ACE2 glycan at N322	271:293	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	2	4	from	N322	290:293	arg1	at					287:288	the ACE2 glycan at N322	271:293	the ACE2 glycan at N322	271:293	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	2	4	from	N322	290:293	arg1	ACE2					275:278	the ACE2 glycan at N322	271:293	the ACE2 glycan at N322	271:293	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	2	4	from	N322	290:293	arg1	glycan					280:285	the ACE2 glycan at N322	271:293	the ACE2 glycan at N322	271:293	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	4	5	gly	glycosylation	619:631	arg2	site					633:636	this glycosylation site	614:636	this glycosylation site	614:636	The absence of this glycosylation site on SARS-CoV-2 RBD may enhance its binding with ACE2.					
34019602	3	6	theme	ACE2	486:489	arg1	N322					501:504	the ACE2 glycan at N322	482:504	the ACE2 glycan at N322	482:504	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	3	7	attach	presence	543:550	arg1	N357					573:576	N357	573:576	N357 of the SARS-CoV RBD	573:596	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	3	7	attach	presence	543:550	arg2	glycan					563:568	the RBD glycan	555:568	the RBD glycan	555:568	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	4	8	theme	SARS-CoV-2	641:650	arg1	RBD					652:654	SARS-CoV-2 RBD	641:654	SARS-CoV-2 RBD	641:654	The absence of this glycosylation site on SARS-CoV-2 RBD may enhance its binding with ACE2.					
34019602	3	9	from	N322	501:504	arg1	glycan					491:496	the ACE2 glycan at N322	482:504	the ACE2 glycan at N322	482:504	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	3	9	from	N322	501:504	arg1	at					498:499	the ACE2 glycan at N322	482:504	the ACE2 glycan at N322	482:504	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	3	9	from	N322	501:504	arg1	ACE2					486:489	the ACE2 glycan at N322	482:504	the ACE2 glycan at N322	482:504	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	3	10	from	N357	573:576	arg1	presence					543:550	the presence	539:550	the presence of the RBD glycan at N357 of the SARS-CoV RBD	539:596	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	3	11	with	interactions	466:477	arg1	RBD					520:522	SARS-CoV RBD	511:522	SARS-CoV RBD	511:522	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	0	12	theme	ACE2	0:3	arg1	glycans					5:11	ACE2 glycans	0:11	ACE2 glycans	0:11	ACE2 glycans preferentially interact with SARS-CoV-2 over SARS-CoV.					
34019602	1	13	with	interactions	107:118	arg1	RBDs					233:236	RBDs	233:236	RBDs	233:236	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	1	13	with	interactions	107:118	arg1	domains					224:230	SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains	173:230	SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs)	173:237	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	1	14	theme	distinct	80:87	arg1	difference					89:98	a distinct difference	78:98	a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs)	78:237	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	4	15	from	absence	603:609	arg1	RBD					652:654	SARS-CoV-2 RBD	641:654	SARS-CoV-2 RBD	641:654	The absence of this glycosylation site on SARS-CoV-2 RBD may enhance its binding with ACE2.					
34019602	2	16	theme	at	363:364	arg1	N90					366:368	the ACE2 glycan at N90	347:368	the ACE2 glycan at N90	347:368	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	2	17	theme	coronaviruses	418:430	arg1	infections					399:408	infections	399:408	infections of both coronaviruses	399:430	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	2	18	theme	glycan	356:361	arg1	N90					366:368	the ACE2 glycan at N90	347:368	the ACE2 glycan at N90	347:368	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	2	19	theme	at	287:288	arg1	N322					290:293	the ACE2 glycan at N322	271:293	the ACE2 glycan at N322	271:293	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	1	20	theme	SARS-CoV-2	173:182	arg1	RBDs					233:236	RBDs	233:236	RBDs	233:236	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	1	20	theme	SARS-CoV-2	173:182	arg1	domains					224:230	SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains	173:230	SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs)	173:237	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	2	21	theme	glycan	280:285	arg1	N322					290:293	the ACE2 glycan at N322	271:293	the ACE2 glycan at N322	271:293	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	3	22	theme	at	498:499	arg1	N322					501:504	the ACE2 glycan at N322	482:504	the ACE2 glycan at N322	482:504	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	4	23	theme	glycosylation	619:631	arg1	site					633:636	this glycosylation site	614:636	this glycosylation site	614:636	The absence of this glycosylation site on SARS-CoV-2 RBD may enhance its binding with ACE2.					
34019602	2	24	with	interactions	304:315	arg1	RBD					337:339	the SARS-CoV-2 RBD	322:339	the SARS-CoV-2 RBD	322:339	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	3	25	theme	RBD	559:561	arg1	glycan					563:568	the RBD glycan	555:568	the RBD glycan	555:568	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	1	26	theme	SARS-CoV	188:195	arg1	S-protein					197:205	SARS-CoV S-protein	188:205	SARS-CoV S-protein	188:205	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	3	27	theme	SARS-CoV	585:592	arg1	RBD					594:596	the SARS-CoV RBD	581:596	the SARS-CoV RBD	581:596	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	3	28	theme	glycan	563:568	arg1	presence					543:550	the presence	539:550	the presence of the RBD glycan at N357 of the SARS-CoV RBD	539:596	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	1	29	theme	S-protein	197:205	arg1	RBDs					233:236	RBDs	233:236	RBDs	233:236	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	1	29	theme	S-protein	197:205	arg1	domains					224:230	SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains	173:230	SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs)	173:237	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	3	30	theme	RBD	594:596	arg1	N357					573:576	N357	573:576	N357 of the SARS-CoV RBD	573:596	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	2	31	theme	SARS-CoV-2	326:335	arg1	RBD					337:339	the SARS-CoV-2 RBD	322:339	the SARS-CoV-2 RBD	322:339	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	3	32	theme	glycan	491:496	arg1	N322					501:504	the ACE2 glycan at N322	482:504	the ACE2 glycan at N322	482:504	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	2	33	from	N90	366:368	arg1	glycan					356:361	the ACE2 glycan at N90	347:368	the ACE2 glycan at N90	347:368	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	2	33	from	N90	366:368	arg1	at					363:364	the ACE2 glycan at N90	347:368	the ACE2 glycan at N90	347:368	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	2	33	from	N90	366:368	arg1	ACE2					351:354	the ACE2 glycan at N90	347:368	the ACE2 glycan at N90	347:368	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	1	34	theme	receptor-binding	207:222	arg1	RBDs					233:236	RBDs	233:236	RBDs	233:236	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	1	34	theme	receptor-binding	207:222	arg1	domains					224:230	SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains	173:230	SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs)	173:237	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	3	35	theme	SARS-CoV	511:518	arg1	RBD					520:522	SARS-CoV RBD	511:522	SARS-CoV RBD	511:522	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
34019602	1	36	theme	glycans	127:133	arg1	interactions					107:118	the interactions	103:118	the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs)	103:237	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	2	37	theme	ACE2	351:354	arg1	N90					366:368	the ACE2 glycan at N90	347:368	the ACE2 glycan at N90	347:368	Our analysis demonstrates that the ACE2 glycan at N322 enhances interactions with the SARS-CoV-2 RBD while the ACE2 glycan at N90 may offer protection against infections of both coronaviruses depending on its composition.					
34019602	1	38	from	difference	89:98	arg1	interactions					107:118	the interactions	103:118	the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs)	103:237	We report a distinct difference in the interactions of the glycans of the host-cell receptor, ACE2, with SARS-CoV-2 and SARS-CoV S-protein receptor-binding domains (RBDs).					
34019602	4	39	with	binding	672:678	arg1	ACE2					685:688	ACE2	685:688	ACE2	685:688	The absence of this glycosylation site on SARS-CoV-2 RBD may enhance its binding with ACE2.					
34019602	3	40	theme	N322	501:504	arg1	interactions					466:477	The interactions	462:477	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD	462:522	The interactions of the ACE2 glycan at N322 with SARS-CoV RBD are blocked by the presence of the RBD glycan at N357 of the SARS-CoV RBD.					
33127550	2	0	theme	Pichia	432:437	arg1	pastoris					439:446	the yeast Pichia pastoris	422:446	the yeast Pichia pastoris	422:446	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	5	1	theme	other	717:721	arg1	hand					723:726	the other hand	713:726	the other hand	713:726	On the other hand, the O-glycosylation retarded the amyloid fibril formation in a sugar size-dependent manner.					
33127550	0	2	theme	domain	70:75	arg1	formation					44:52	amyloid fibril formation	29:52	amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil	29:109	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	1	3	theme	amyloid	235:241	arg1	formation					250:258	the amyloid fibril formation	231:258	the amyloid fibril formation in several amyloidogenic proteins and peptides	231:305	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	0	4	theme	Vλ6	84:86	arg1	chain					94:98	Vλ6 light chain	84:98	the Vλ6 light chain mutant Wil	80:109	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	0	5	from	Effect	0:5	arg1	formation					44:52	amyloid fibril formation	29:52	amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil	29:109	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	3	6	theme	non-glycosylated	536:551	arg1	mutants					557:563	the O-glycosylated and non-glycosylated Wil mutants	513:563	the O-glycosylated and non-glycosylated Wil mutants in high yield	513:577	After separation by cation exchange chromatography, we obtained the O-glycosylated and non-glycosylated Wil mutants in high yield.					
33127550	1	7	theme	fibril	243:248	arg1	formation					250:258	the amyloid fibril formation	231:258	the amyloid fibril formation in several amyloidogenic proteins and peptides	231:305	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	6	8	theme	formation	930:938	arg1	retardation					900:910	the retardation	896:910	the retardation of amyloid fibril formation	896:938	From these results, we discussed the role of covalently attached glycan in the retardation of amyloid fibril formation.					
33127550	0	9	theme	chain	94:98	arg1	Wil					107:109	the Vλ6 light chain mutant Wil	80:109	the Vλ6 light chain mutant Wil	80:109	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	0	10	from	formation	44:52	arg1	Wil					107:109	the Vλ6 light chain mutant Wil	80:109	the Vλ6 light chain mutant Wil	80:109	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	3	11	theme	exchange	476:483	arg1	chromatography					485:498	cation exchange chromatography	469:498	cation exchange chromatography	469:498	After separation by cation exchange chromatography, we obtained the O-glycosylated and non-glycosylated Wil mutants in high yield.					
33127550	1	12	theme	major	140:144	arg1	modifications					165:177	the major post-translational modifications	136:177	the major post-translational modifications in eukaryotic cells	136:197	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	0	13	theme	light	88:92	arg1	chain					94:98	Vλ6 light chain	84:98	the Vλ6 light chain mutant Wil	80:109	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	1	14	theme	post-translational	146:163	arg1	modifications					165:177	the major post-translational modifications	136:177	the major post-translational modifications in eukaryotic cells	136:197	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	2	15	theme	yeast	426:430	arg1	pastoris					439:446	the yeast Pichia pastoris	422:446	the yeast Pichia pastoris	422:446	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	6	16	from	role	858:861	arg1	retardation					900:910	the retardation	896:910	the retardation of amyloid fibril formation	896:938	From these results, we discussed the role of covalently attached glycan in the retardation of amyloid fibril formation.					
33127550	3	17	theme	O-glycosylated	517:530	arg1	mutants					557:563	the O-glycosylated and non-glycosylated Wil mutants	513:563	the O-glycosylated and non-glycosylated Wil mutants in high yield	513:577	After separation by cation exchange chromatography, we obtained the O-glycosylated and non-glycosylated Wil mutants in high yield.					
33127550	1	18	theme	modifications	165:177	arg1	modifications					165:177	the major post-translational modifications	136:177	the major post-translational modifications in eukaryotic cells	136:197	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	1	18	theme	modifications	165:177	arg1	one					129:131	one	129:131	one	129:131	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	5	19	theme	amyloid	762:768	arg1	formation					777:785	the amyloid fibril formation	758:785	the amyloid fibril formation	758:785	On the other hand, the O-glycosylation retarded the amyloid fibril formation in a sugar size-dependent manner.					
33127550	6	20	theme	fibril	923:928	arg1	formation					930:938	amyloid fibril formation	915:938	amyloid fibril formation	915:938	From these results, we discussed the role of covalently attached glycan in the retardation of amyloid fibril formation.					
33127550	2	21	theme	chain	348:352	arg1	Wil					362:364	Wil	362:364	Wil	362:364	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	2	21	theme	chain	348:352	arg1	mutant					354:359	a Vλ6 light chain mutant	336:359	a Vλ6 light chain mutant	336:359	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	2	21	theme	chain	348:352	arg1	mutant					393:398	an amyloidogenic mutant	376:398	an amyloidogenic mutant in AL amyloidosis	376:416	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	5	22	theme	fibril	770:775	arg1	formation					777:785	the amyloid fibril formation	758:785	the amyloid fibril formation	758:785	On the other hand, the O-glycosylation retarded the amyloid fibril formation in a sugar size-dependent manner.					
33127550	1	23	theme	several	263:269	arg1	proteins					285:292	several amyloidogenic proteins	263:292	several amyloidogenic proteins	263:292	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	1	24	theme	eukaryotic	182:191	arg1	cells					193:197	eukaryotic cells	182:197	eukaryotic cells	182:197	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	0	25	theme	O-glycosylation	10:24	arg1	Effect					0:5	Effect	0:5	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.	0:110	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	1	26	theme	amyloidogenic	271:283	arg1	proteins					285:292	several amyloidogenic proteins	263:292	several amyloidogenic proteins	263:292	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	0	27	theme	mutant	100:105	arg1	Wil					107:109	the Vλ6 light chain mutant Wil	80:109	the Vλ6 light chain mutant Wil	80:109	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	3	28	from	mutants	557:563	arg1	yield					573:577	high yield	568:577	high yield	568:577	After separation by cation exchange chromatography, we obtained the O-glycosylated and non-glycosylated Wil mutants in high yield.					
33127550	2	29	theme	light	342:346	arg1	Wil					362:364	Wil	362:364	Wil	362:364	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	2	29	theme	light	342:346	arg1	mutant					354:359	a Vλ6 light chain mutant	336:359	a Vλ6 light chain mutant	336:359	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	2	29	theme	light	342:346	arg1	mutant					393:398	an amyloidogenic mutant	376:398	an amyloidogenic mutant in AL amyloidosis	376:416	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	0	30	theme	fibril	37:42	arg1	formation					44:52	amyloid fibril formation	29:52	amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil	29:109	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	5	31	theme	sugar	792:796	arg1	manner					813:818	a sugar size-dependent manner	790:818	a sugar size-dependent manner	790:818	On the other hand, the O-glycosylation retarded the amyloid fibril formation in a sugar size-dependent manner.					
33127550	4	32	theme	Wil	604:606	arg1	mutants					608:614	these Wil mutants	598:614	these Wil mutants	598:614	The structures of these Wil mutants were identical except with respect to glycosylation, and the stabilities were also identical.					
33127550	0	33	theme	amyloid	29:35	arg1	formation					44:52	amyloid fibril formation	29:52	amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil	29:109	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	3	34	gly	non-glycosylated	536:551	arg1	mutants					557:563	the O-glycosylated and non-glycosylated Wil mutants	513:563	the O-glycosylated and non-glycosylated Wil mutants in high yield	513:577	After separation by cation exchange chromatography, we obtained the O-glycosylated and non-glycosylated Wil mutants in high yield.					
33127550	6	35	theme	amyloid	915:921	arg1	formation					930:938	amyloid fibril formation	915:938	amyloid fibril formation	915:938	From these results, we discussed the role of covalently attached glycan in the retardation of amyloid fibril formation.					
33127550	0	36	gly	O-glycosylation	10:24	arg1	domain					70:75	the variable domain	57:75	the variable domain	57:75	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	0	36	gly	O-glycosylation	10:24	arg1	fibril					37:42	amyloid fibril formation	29:52	amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil	29:109	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	6	37	theme	glycan	886:891	arg1	role					858:861	the role	854:861	the role of covalently attached glycan in the retardation of amyloid fibril formation	854:938	From these results, we discussed the role of covalently attached glycan in the retardation of amyloid fibril formation.					
33127550	3	38	theme	Wil	553:555	arg1	mutants					557:563	the O-glycosylated and non-glycosylated Wil mutants	513:563	the O-glycosylated and non-glycosylated Wil mutants in high yield	513:577	After separation by cation exchange chromatography, we obtained the O-glycosylated and non-glycosylated Wil mutants in high yield.					
33127550	3	39	gly	O-glycosylated	517:530	arg1	mutants					557:563	the O-glycosylated and non-glycosylated Wil mutants	513:563	the O-glycosylated and non-glycosylated Wil mutants in high yield	513:577	After separation by cation exchange chromatography, we obtained the O-glycosylated and non-glycosylated Wil mutants in high yield.					
33127550	6	40	theme	attached	877:884	arg1	glycan					886:891	covalently attached glycan	866:891	covalently attached glycan	866:891	From these results, we discussed the role of covalently attached glycan in the retardation of amyloid fibril formation.					
33127550	1	41	from	modifications	165:177	arg1	cells					193:197	eukaryotic cells	182:197	eukaryotic cells	182:197	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	3	42	theme	cation	469:474	arg1	chromatography					485:498	cation exchange chromatography	469:498	cation exchange chromatography	469:498	After separation by cation exchange chromatography, we obtained the O-glycosylated and non-glycosylated Wil mutants in high yield.					
33127550	0	43	theme	variable	61:68	arg1	domain					70:75	the variable domain	57:75	the variable domain	57:75	Effect of O-glycosylation on amyloid fibril formation of the variable domain in the Vλ6 light chain mutant Wil.					
33127550	5	44	theme	size-dependent	798:811	arg1	manner					813:818	a sugar size-dependent manner	790:818	a sugar size-dependent manner	790:818	On the other hand, the O-glycosylation retarded the amyloid fibril formation in a sugar size-dependent manner.					
33127550	3	45	theme	high	568:571	arg1	yield					573:577	high yield	568:577	high yield	568:577	After separation by cation exchange chromatography, we obtained the O-glycosylated and non-glycosylated Wil mutants in high yield.					
33127550	1	46	from	formation	250:258	arg1	peptides					298:305	peptides	298:305	peptides	298:305	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	1	46	from	formation	250:258	arg1	proteins					285:292	several amyloidogenic proteins	263:292	several amyloidogenic proteins	263:292	Glycosylation is one of the major post-translational modifications in eukaryotic cells and has been reported to affect the amyloid fibril formation in several amyloidogenic proteins and peptides.					
33127550	4	47	theme	mutants	608:614	arg1	structures					584:593	The structures	580:593	The structures of these Wil mutants	580:614	The structures of these Wil mutants were identical except with respect to glycosylation, and the stabilities were also identical.					
33127550	4	47	theme	mutants	608:614	arg1	identical					621:629	identical	621:629	identical	621:629	The structures of these Wil mutants were identical except with respect to glycosylation, and the stabilities were also identical.					
33127550	2	48	from	mutant	393:398	arg1	amyloidosis					406:416	AL amyloidosis	403:416	AL amyloidosis	403:416	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	2	49	theme	amyloidogenic	379:391	arg1	mutant					393:398	an amyloidogenic mutant	376:398	an amyloidogenic mutant in AL amyloidosis	376:416	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	2	49	theme	amyloidogenic	379:391	arg1	mutant					354:359	a Vλ6 light chain mutant	336:359	a Vλ6 light chain mutant	336:359	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	2	50	theme	Vλ6	338:340	arg1	Wil					362:364	Wil	362:364	Wil	362:364	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	2	50	theme	Vλ6	338:340	arg1	mutant					354:359	a Vλ6 light chain mutant	336:359	a Vλ6 light chain mutant	336:359	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33127550	2	50	theme	Vλ6	338:340	arg1	mutant					393:398	an amyloidogenic mutant	376:398	an amyloidogenic mutant in AL amyloidosis	376:416	In this study, we expressed a Vλ6 light chain mutant, Wil, which is an amyloidogenic mutant in AL amyloidosis, by the yeast Pichia pastoris.					
33835779	0	0	theme	Engineered	91:100	arg1	Pyrophosphorylase					102:118	an Engineered Pyrophosphorylase	88:118	an Engineered Pyrophosphorylase	88:118	Optimization of Metabolic Oligosaccharide Engineering with Ac4GalNAlk and Ac4GlcNAlk by an Engineered Pyrophosphorylase.					
33835779	2	1	gly	glycoproteins	356:368	arg1	glycoproteins					356:368	glycoproteins	356:368	glycoproteins	356:368	Efficient MOE reagents are activated into nucleotide-sugars by cellular biosynthetic machineries, introduced into glycoproteins and traceable by bioorthogonal chemistry.					
33835779	7	2	theme	known	1089:1093	arg1	reagents					1112:1119	known azide-tagged MOE reagents	1089:1119	known azide-tagged MOE reagents	1089:1119	A comparison with known azide-tagged MOE reagents reveals major differences in glycoprotein labeling, substantially expanding the toolbox of chemical glycobiology.					
33835779	7	3	with	comparison	1073:1082	arg1	reagents					1112:1119	known azide-tagged MOE reagents	1089:1119	known azide-tagged MOE reagents	1089:1119	A comparison with known azide-tagged MOE reagents reveals major differences in glycoprotein labeling, substantially expanding the toolbox of chemical glycobiology.					
33835779	6	4	theme	engineered	901:910	arg1	version					912:918	an engineered version	898:918	an engineered version of the pyrophosphorylase AGX1	898:948	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	4	5	theme	poor	578:581	arg1	uptake					583:588	poor uptake	578:588	poor uptake by biosynthetic salvage pathways	578:621	While metabolic interconnectivity can affect probe specificity, poor uptake by biosynthetic salvage pathways may impact probe sensitivity and trigger side reactions.					
33835779	5	6	theme	weak	727:730	arg1	Ac4GalNAlk					759:768	Ac4GalNAlk	759:768	Ac4GalNAlk	759:768	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	5	6	theme	weak	727:730	arg1	reagents					750:757	the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk	723:783	the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk	723:783	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	5	6	theme	weak	727:730	arg1	Ac4GlcNAlk					774:783	Ac4GlcNAlk	774:783	Ac4GlcNAlk	774:783	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	6	7	theme	cell	1015:1018	arg1	labeling					1028:1035	bioorthogonal cell surface labeling	1001:1035	bioorthogonal cell surface labeling by up to two orders of magnitude	1001:1068	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	5	8	theme	alkyne-tagged	732:744	arg1	Ac4GalNAlk					759:768	Ac4GalNAlk	759:768	Ac4GalNAlk	759:768	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	5	8	theme	alkyne-tagged	732:744	arg1	reagents					750:757	the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk	723:783	the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk	723:783	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	5	8	theme	alkyne-tagged	732:744	arg1	Ac4GlcNAlk					774:783	Ac4GlcNAlk	774:783	Ac4GlcNAlk	774:783	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	4	9	theme	side	664:667	arg1	reactions					669:677	side reactions	664:677	side reactions	664:677	While metabolic interconnectivity can affect probe specificity, poor uptake by biosynthetic salvage pathways may impact probe sensitivity and trigger side reactions.					
33835779	5	10	dep	reagents	750:757	arg1	Ac4GalNAlk					759:768	Ac4GalNAlk	759:768	Ac4GalNAlk	759:768	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	5	10	dep	reagents	750:757	arg1	reagents					750:757	the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk	723:783	the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk	723:783	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	5	10	dep	reagents	750:757	arg1	Ac4GlcNAlk					774:783	Ac4GlcNAlk	774:783	Ac4GlcNAlk	774:783	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	7	11	theme	glycobiology	1221:1232	arg1	toolbox					1201:1207	the toolbox	1197:1207	the toolbox of chemical glycobiology	1197:1232	A comparison with known azide-tagged MOE reagents reveals major differences in glycoprotein labeling, substantially expanding the toolbox of chemical glycobiology.					
33835779	3	12	theme	widespread	426:435	arg1	use					437:439	their widespread use	420:439	their widespread use	420:439	Despite their widespread use, the metabolic fate of many MOE reagents is only beginning to be mapped.					
33835779	4	13	theme	probe	559:563	arg1	specificity					565:575	probe specificity	559:575	probe specificity	559:575	While metabolic interconnectivity can affect probe specificity, poor uptake by biosynthetic salvage pathways may impact probe sensitivity and trigger side reactions.					
33835779	5	14	theme	metabolic	693:701	arg1	engineering					703:713	metabolic engineering	693:713	metabolic engineering	693:713	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	6	15	theme	metabolic	872:880	arg1	bottleneck					882:891	a metabolic bottleneck	870:891	a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1	870:948	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	6	16	theme	magnitude	1060:1068	arg1	orders					1050:1055	up to two orders	1040:1055	up to two orders of magnitude	1040:1068	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	6	17	theme	bioorthogonal	1001:1013	arg1	labeling					1028:1035	bioorthogonal cell surface labeling	1001:1035	bioorthogonal cell surface labeling by up to two orders of magnitude	1001:1068	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	6	18	with	bottleneck	882:891	arg1	version					912:918	an engineered version	898:918	an engineered version of the pyrophosphorylase AGX1	898:948	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	7	19	theme	chemical	1212:1219	arg1	glycobiology					1221:1232	chemical glycobiology	1212:1232	chemical glycobiology	1212:1232	A comparison with known azide-tagged MOE reagents reveals major differences in glycoprotein labeling, substantially expanding the toolbox of chemical glycobiology.					
33835779	6	20	theme	pyrophosphorylase	927:943	arg1	AGX1					945:948	the pyrophosphorylase AGX1	923:948	the pyrophosphorylase AGX1	923:948	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	1	21	theme	protein	219:225	arg1	glycosylation					227:239	protein glycosylation	219:239	protein glycosylation	219:239	Metabolic oligosaccharide engineering (MOE) has fundamentally contributed to our understanding of protein glycosylation.					
33835779	0	22	theme	Oligosaccharide	26:40	arg1	Engineering					42:52	Metabolic Oligosaccharide Engineering	16:52	Metabolic Oligosaccharide Engineering	16:52	Optimization of Metabolic Oligosaccharide Engineering with Ac4GalNAlk and Ac4GlcNAlk by an Engineered Pyrophosphorylase.					
33835779	1	23	theme	Metabolic	121:129	arg1	MOE					160:162	MOE	160:162	MOE	160:162	Metabolic oligosaccharide engineering (MOE) has fundamentally contributed to our understanding of protein glycosylation.					
33835779	1	23	theme	Metabolic	121:129	arg1	engineering					147:157	Metabolic oligosaccharide engineering	121:157	Metabolic oligosaccharide engineering (MOE)	121:163	Metabolic oligosaccharide engineering (MOE) has fundamentally contributed to our understanding of protein glycosylation.					
33835779	1	24	theme	glycosylation	227:239	arg1	understanding					202:214	our understanding	198:214	our understanding of protein glycosylation	198:239	Metabolic oligosaccharide engineering (MOE) has fundamentally contributed to our understanding of protein glycosylation.					
33835779	3	25	theme	metabolic	446:454	arg1	fate					456:459	the metabolic fate	442:459	the metabolic fate of many MOE reagents	442:480	Despite their widespread use, the metabolic fate of many MOE reagents is only beginning to be mapped.					
33835779	6	26	dep	two	1046:1048	arg1	to					1043:1044	to	1043:1044	to	1043:1044	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	1	27	theme	oligosaccharide	131:145	arg1	MOE					160:162	MOE	160:162	MOE	160:162	Metabolic oligosaccharide engineering (MOE) has fundamentally contributed to our understanding of protein glycosylation.					
33835779	1	27	theme	oligosaccharide	131:145	arg1	engineering					147:157	Metabolic oligosaccharide engineering	121:157	Metabolic oligosaccharide engineering (MOE)	121:163	Metabolic oligosaccharide engineering (MOE) has fundamentally contributed to our understanding of protein glycosylation.					
33835779	3	28	theme	reagents	473:480	arg1	fate					456:459	the metabolic fate	442:459	the metabolic fate of many MOE reagents	442:480	Despite their widespread use, the metabolic fate of many MOE reagents is only beginning to be mapped.					
33835779	4	29	theme	probe	634:638	arg1	sensitivity					640:650	probe sensitivity	634:650	probe sensitivity	634:650	While metabolic interconnectivity can affect probe specificity, poor uptake by biosynthetic salvage pathways may impact probe sensitivity and trigger side reactions.					
33835779	5	30	theme	efficient	790:798	arg1	tools					809:813	efficient chemical tools	790:813	efficient chemical tools to probe protein glycosylation	790:844	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	6	31	theme	nucleotide-sugar	957:972	arg1	biosynthesis					974:985	nucleotide-sugar biosynthesis	957:985	nucleotide-sugar biosynthesis	957:985	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	0	32	theme	Engineering	42:52	arg1	Optimization					0:11	Optimization	0:11	Optimization of Metabolic Oligosaccharide Engineering with Ac4GalNAlk and Ac4GlcNAlk by an Engineered Pyrophosphorylase.	0:119	Optimization of Metabolic Oligosaccharide Engineering with Ac4GalNAlk and Ac4GlcNAlk by an Engineered Pyrophosphorylase.					
33835779	4	33	theme	biosynthetic	593:604	arg1	pathways					614:621	biosynthetic salvage pathways	593:621	biosynthetic salvage pathways	593:621	While metabolic interconnectivity can affect probe specificity, poor uptake by biosynthetic salvage pathways may impact probe sensitivity and trigger side reactions.					
33835779	5	34	theme	chemical	800:807	arg1	tools					809:813	efficient chemical tools	790:813	efficient chemical tools to probe protein glycosylation	790:844	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	4	35	theme	metabolic	520:528	arg1	interconnectivity					530:546	metabolic interconnectivity	520:546	metabolic interconnectivity	520:546	While metabolic interconnectivity can affect probe specificity, poor uptake by biosynthetic salvage pathways may impact probe sensitivity and trigger side reactions.					
33835779	7	36	theme	major	1129:1133	arg1	differences					1135:1145	major differences	1129:1145	major differences in glycoprotein labeling	1129:1170	A comparison with known azide-tagged MOE reagents reveals major differences in glycoprotein labeling, substantially expanding the toolbox of chemical glycobiology.					
33835779	5	37	theme	protein	824:830	arg1	glycosylation					832:844	protein glycosylation	824:844	protein glycosylation	824:844	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	3	38	theme	many	464:467	arg1	reagents					473:480	many MOE reagents	464:480	many MOE reagents	464:480	Despite their widespread use, the metabolic fate of many MOE reagents is only beginning to be mapped.					
33835779	5	39	theme	MOE	746:748	arg1	Ac4GalNAlk					759:768	Ac4GalNAlk	759:768	Ac4GalNAlk	759:768	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	5	39	theme	MOE	746:748	arg1	reagents					750:757	the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk	723:783	the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk	723:783	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	5	39	theme	MOE	746:748	arg1	Ac4GlcNAlk					774:783	Ac4GlcNAlk	774:783	Ac4GlcNAlk	774:783	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	6	40	theme	AGX1	945:948	arg1	version					912:918	an engineered version	898:918	an engineered version of the pyrophosphorylase AGX1	898:948	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	3	41	theme	MOE	469:471	arg1	reagents					473:480	many MOE reagents	464:480	many MOE reagents	464:480	Despite their widespread use, the metabolic fate of many MOE reagents is only beginning to be mapped.					
33835779	2	42	theme	MOE	252:254	arg1	reagents					256:263	Efficient MOE reagents	242:263	Efficient MOE reagents	242:263	Efficient MOE reagents are activated into nucleotide-sugars by cellular biosynthetic machineries, introduced into glycoproteins and traceable by bioorthogonal chemistry.					
33835779	4	43	theme	salvage	606:612	arg1	pathways					614:621	biosynthetic salvage pathways	593:621	biosynthetic salvage pathways	593:621	While metabolic interconnectivity can affect probe specificity, poor uptake by biosynthetic salvage pathways may impact probe sensitivity and trigger side reactions.					
33835779	5	44	dep	tools	809:813	arg1	probe					818:822	probe	818:822	to probe protein glycosylation	815:844	Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac4GalNAlk and Ac4GlcNAlk into efficient chemical tools to probe protein glycosylation.					
33835779	2	45	theme	Efficient	242:250	arg1	reagents					256:263	Efficient MOE reagents	242:263	Efficient MOE reagents	242:263	Efficient MOE reagents are activated into nucleotide-sugars by cellular biosynthetic machineries, introduced into glycoproteins and traceable by bioorthogonal chemistry.					
33835779	2	46	theme	biosynthetic	314:325	arg1	machineries					327:337	cellular biosynthetic machineries	305:337	cellular biosynthetic machineries	305:337	Efficient MOE reagents are activated into nucleotide-sugars by cellular biosynthetic machineries, introduced into glycoproteins and traceable by bioorthogonal chemistry.					
33835779	0	47	with	Optimization	0:11	arg1	Ac4GlcNAlk					74:83	Ac4GlcNAlk	74:83	Ac4GlcNAlk	74:83	Optimization of Metabolic Oligosaccharide Engineering with Ac4GalNAlk and Ac4GlcNAlk by an Engineered Pyrophosphorylase.					
33835779	0	47	with	Optimization	0:11	arg1	Ac4GalNAlk					59:68	Ac4GalNAlk	59:68	Ac4GalNAlk	59:68	Optimization of Metabolic Oligosaccharide Engineering with Ac4GalNAlk and Ac4GlcNAlk by an Engineered Pyrophosphorylase.					
33835779	7	48	gly	glycoprotein	1150:1161	arg1	glycoprotein					1150:1161	glycoprotein labeling	1150:1170	glycoprotein labeling	1150:1170	A comparison with known azide-tagged MOE reagents reveals major differences in glycoprotein labeling, substantially expanding the toolbox of chemical glycobiology.					
33835779	7	49	theme	glycoprotein	1150:1161	arg1	labeling					1163:1170	glycoprotein labeling	1150:1170	glycoprotein labeling	1150:1170	A comparison with known azide-tagged MOE reagents reveals major differences in glycoprotein labeling, substantially expanding the toolbox of chemical glycobiology.					
33835779	2	50	theme	cellular	305:312	arg1	machineries					327:337	cellular biosynthetic machineries	305:337	cellular biosynthetic machineries	305:337	Efficient MOE reagents are activated into nucleotide-sugars by cellular biosynthetic machineries, introduced into glycoproteins and traceable by bioorthogonal chemistry.					
33835779	7	51	theme	azide-tagged	1095:1106	arg1	reagents					1112:1119	known azide-tagged MOE reagents	1089:1119	known azide-tagged MOE reagents	1089:1119	A comparison with known azide-tagged MOE reagents reveals major differences in glycoprotein labeling, substantially expanding the toolbox of chemical glycobiology.					
33835779	2	52	theme	bioorthogonal	387:399	arg1	chemistry					401:409	bioorthogonal chemistry	387:409	bioorthogonal chemistry	387:409	Efficient MOE reagents are activated into nucleotide-sugars by cellular biosynthetic machineries, introduced into glycoproteins and traceable by bioorthogonal chemistry.					
33835779	7	53	from	differences	1135:1145	arg1	labeling					1163:1170	glycoprotein labeling	1150:1170	glycoprotein labeling	1150:1170	A comparison with known azide-tagged MOE reagents reveals major differences in glycoprotein labeling, substantially expanding the toolbox of chemical glycobiology.					
33835779	7	54	theme	MOE	1108:1110	arg1	reagents					1112:1119	known azide-tagged MOE reagents	1089:1119	known azide-tagged MOE reagents	1089:1119	A comparison with known azide-tagged MOE reagents reveals major differences in glycoprotein labeling, substantially expanding the toolbox of chemical glycobiology.					
33835779	6	55	theme	surface	1020:1026	arg1	labeling					1028:1035	bioorthogonal cell surface labeling	1001:1035	bioorthogonal cell surface labeling by up to two orders of magnitude	1001:1068	We find that bypassing a metabolic bottleneck with an engineered version of the pyrophosphorylase AGX1 boosts nucleotide-sugar biosynthesis and increases bioorthogonal cell surface labeling by up to two orders of magnitude.					
33835779	0	56	theme	Metabolic	16:24	arg1	Engineering					42:52	Metabolic Oligosaccharide Engineering	16:52	Metabolic Oligosaccharide Engineering	16:52	Optimization of Metabolic Oligosaccharide Engineering with Ac4GalNAlk and Ac4GlcNAlk by an Engineered Pyrophosphorylase.					
32129075	0	0	theme	TiO2-PGC	80:87	arg1	Spectrometry					99:110	TiO2-PGC Chip Mass Spectrometry	80:110	TiO2-PGC Chip Mass Spectrometry	80:110	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.					
32129075	1	1	theme	diseases	301:308	arg1	study					269:273	the study	265:273	the study of human viral infectious diseases	265:308	Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases.					
32129075	5	2	theme	high	710:713	arg1	sensitivity					715:725	a high sensitivity and accurate MRM method	708:749	sensitivity	715:725	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	2	3	from	profile	374:380	arg1	shrews					423:428	tree shrews	418:428	tree shrews	418:428	However, there is still no report for the comprehensive glycan profile of the respiratory tract tissues in tree shrews.					
32129075	9	4	theme	influenza	1339:1347	arg1	infection					1349:1357	human influenza infection	1333:1357	human influenza infection	1333:1357	Our results also help to better understand the role of glycan receptors in human influenza infection and pathogenesis.					
32129075	8	5	theme	shrews	1250:1255	arg1	tract					1236:1240	the respiratory tract	1220:1240	the respiratory tract of tree shrews	1220:1255	This study is the first to reveal the comprehensive glycomic profile of the respiratory tract of tree shrews.					
32129075	2	6	from	tissues	407:413	arg1	shrews					423:428	tree shrews	418:428	tree shrews	418:428	However, there is still no report for the comprehensive glycan profile of the respiratory tract tissues in tree shrews.					
32129075	6	7	from	bronchus	1002:1009	arg1	composition					901:911	the N-glycan composition	888:911	the N-glycan composition in the turbinate and lung	888:937	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	6	7	from	bronchus	1002:1009	arg1	different					957:965	different	957:965	different	957:965	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	5	8	theme	MS	850:851	arg1	detection					853:861	MS detection	850:861	MS detection	850:861	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	9	9	from	role	1305:1308	arg1	infection					1349:1357	human influenza infection	1333:1357	human influenza infection	1333:1357	Our results also help to better understand the role of glycan receptors in human influenza infection and pathogenesis.					
32129075	9	9	from	role	1305:1308	arg1	pathogenesis					1363:1374	pathogenesis	1363:1374	pathogenesis	1363:1374	Our results also help to better understand the role of glycan receptors in human influenza infection and pathogenesis.					
32129075	0	10	theme	Mass	94:97	arg1	Spectrometry					99:110	TiO2-PGC Chip Mass Spectrometry	80:110	TiO2-PGC Chip Mass Spectrometry	80:110	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.					
32129075	3	11	theme	N-glycans	491:499	arg1	diversity					478:486	the structural diversity	463:486	the structural diversity of N-glycans in the respiratory tract of tree shrews	463:539	In this study, we characterized the structural diversity of N-glycans in the respiratory tract of tree shrews using our well-established TiO2-PGC chip-Q-TOF-MS method.					
32129075	1	12	theme	ideal	230:234	arg1	model					255:259	an ideal alternative animal model	227:259	an ideal alternative animal model for the study of human viral infectious diseases	227:308	Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases.					
32129075	0	13	theme	Chip	89:92	arg1	Spectrometry					99:110	TiO2-PGC Chip Mass Spectrometry	80:110	TiO2-PGC Chip Mass Spectrometry	80:110	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.					
32129075	1	14	theme	alternative	236:246	arg1	model					255:259	an ideal alternative animal model	227:259	an ideal alternative animal model for the study of human viral infectious diseases	227:308	Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases.					
32129075	7	15	theme	virus	1120:1124	arg1	infection					1102:1110	the infection	1098:1110	the infection of H1N1 virus A/California/04/2009	1098:1145	Meanwhile, 28 high-level N-glycans in turbinate were speculated to be correlated with the infection of H1N1 virus A/California/04/2009.					
32129075	7	16	theme	high-level	1026:1035	arg1	N-glycans					1037:1045	28 high-level N-glycans	1023:1045	28 high-level N-glycans in turbinate	1023:1058	Meanwhile, 28 high-level N-glycans in turbinate were speculated to be correlated with the infection of H1N1 virus A/California/04/2009.					
32129075	7	16	theme	high-level	1026:1035	arg1	Meanwhile					1012:1020	Meanwhile	1012:1020	Meanwhile	1012:1020	Meanwhile, 28 high-level N-glycans in turbinate were speculated to be correlated with the infection of H1N1 virus A/California/04/2009.					
32129075	5	17	from	variation	837:845	arg1	detection					853:861	MS detection	850:861	MS detection	850:861	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	7	18	theme	H1N1	1115:1118	arg1	virus					1120:1124	H1N1 virus A/California/04/2009	1115:1145	H1N1 virus A/California/04/2009	1115:1145	Meanwhile, 28 high-level N-glycans in turbinate were speculated to be correlated with the infection of H1N1 virus A/California/04/2009.					
32129075	0	19	theme	Comprehensive	0:12	arg1	Profiling					23:31	Comprehensive Glycomic Profiling	0:31	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.	0:111	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.					
32129075	0	20	theme	Glycomic	14:21	arg1	Profiling					23:31	Comprehensive Glycomic Profiling	0:31	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.	0:111	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.					
32129075	2	21	from	shrews	423:428	arg1	profile					374:380	the comprehensive glycan profile	349:380	the comprehensive glycan profile of the respiratory tract tissues in tree shrews	349:428	However, there is still no report for the comprehensive glycan profile of the respiratory tract tissues in tree shrews.					
32129075	6	22	from	palate	981:986	arg1	composition					901:911	the N-glycan composition	888:911	the N-glycan composition in the turbinate and lung	888:937	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	6	22	from	palate	981:986	arg1	different					957:965	different	957:965	different	957:965	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	8	23	theme	comprehensive	1186:1198	arg1	profile					1209:1215	the comprehensive glycomic profile	1182:1215	the comprehensive glycomic profile of the respiratory tract of tree shrews	1182:1255	This study is the first to reveal the comprehensive glycomic profile of the respiratory tract of tree shrews.					
32129075	2	24	theme	tract	401:405	arg1	tissues					407:413	the respiratory tract tissues	385:413	the respiratory tract tissues in tree shrews	385:428	However, there is still no report for the comprehensive glycan profile of the respiratory tract tissues in tree shrews.					
32129075	1	25	theme	animal	248:253	arg1	model					255:259	an ideal alternative animal model	227:259	an ideal alternative animal model for the study of human viral infectious diseases	227:308	Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases.					
32129075	5	26	theme	identified	669:678	arg1	N-glycan					680:687	each identified N-glycan	664:687	each identified N-glycan	664:687	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	5	27	theme	inherent	817:824	arg1	variation					837:845	the inherent run-to-run variation	813:845	the inherent run-to-run variation in MS detection	813:861	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	2	28	theme	respiratory	389:399	arg1	tissues					407:413	the respiratory tract tissues	385:413	the respiratory tract tissues in tree shrews	385:428	However, there is still no report for the comprehensive glycan profile of the respiratory tract tissues in tree shrews.					
32129075	6	29	from	trachea	989:995	arg1	composition					901:911	the N-glycan composition	888:911	the N-glycan composition in the turbinate and lung	888:937	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	6	29	from	trachea	989:995	arg1	different					957:965	different	957:965	different	957:965	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	5	30	theme	MRM	740:742	arg1	method					744:749	a high sensitivity and accurate MRM method	708:749	method	744:749	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	5	31	theme	internal	773:780	arg1	standards					782:790	13C-labeled internal standards	761:790	13C-labeled internal standards	761:790	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	5	32	theme	13C-labeled	761:771	arg1	standards					782:790	13C-labeled internal standards	761:790	13C-labeled internal standards	761:790	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	6	33	from	composition	901:911	arg1	lung					934:937	lung	934:937	lung	934:937	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	6	33	from	composition	901:911	arg1	turbinate					920:928	turbinate	920:928	turbinate	920:928	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	3	34	theme	respiratory	508:518	arg1	tract					520:524	the respiratory tract	504:524	the respiratory tract of tree shrews	504:539	In this study, we characterized the structural diversity of N-glycans in the respiratory tract of tree shrews using our well-established TiO2-PGC chip-Q-TOF-MS method.					
32129075	4	35	theme	N-glycans	627:635	arg1	result					604:609	a result	602:609	a result	602:609	As a result, a total of 219 N-glycans were identified.					
32129075	4	35	theme	N-glycans	627:635	arg1	total					614:618	a total	612:618	a total of 219 N-glycans	612:635	As a result, a total of 219 N-glycans were identified.					
32129075	0	36	theme	Tract	48:52	arg1	Tissues					54:60	Respiratory Tract Tissues	36:60	Respiratory Tract Tissues of Tree Shrews	36:75	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.					
32129075	7	37	from	N-glycans	1037:1045	arg1	turbinate					1050:1058	turbinate	1050:1058	turbinate	1050:1058	Meanwhile, 28 high-level N-glycans in turbinate were speculated to be correlated with the infection of H1N1 virus A/California/04/2009.					
32129075	1	38	theme	human	189:193	arg1	viruses					195:201	human viruses	189:201	human viruses	189:201	Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases.					
32129075	8	39	theme	glycomic	1200:1207	arg1	profile					1209:1215	the comprehensive glycomic profile	1182:1215	the comprehensive glycomic profile of the respiratory tract of tree shrews	1182:1255	This study is the first to reveal the comprehensive glycomic profile of the respiratory tract of tree shrews.					
32129075	2	40	theme	glycan	367:372	arg1	profile					374:380	the comprehensive glycan profile	349:380	the comprehensive glycan profile of the respiratory tract tissues in tree shrews	349:428	However, there is still no report for the comprehensive glycan profile of the respiratory tract tissues in tree shrews.					
32129075	0	41	theme	Respiratory	36:46	arg1	Tissues					54:60	Respiratory Tract Tissues	36:60	Respiratory Tract Tissues of Tree Shrews	36:75	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.					
32129075	6	42	theme	N-glycan	892:899	arg1	different					957:965	different	957:965	different	957:965	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	6	42	theme	N-glycan	892:899	arg1	composition					901:911	the N-glycan composition	888:911	the N-glycan composition in the turbinate and lung	888:937	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	5	43	theme	accurate	731:738	arg1	method					744:749	a high sensitivity and accurate MRM method	708:749	method	744:749	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	2	44	theme	comprehensive	353:365	arg1	profile					374:380	the comprehensive glycan profile	349:380	the comprehensive glycan profile of the respiratory tract tissues in tree shrews	349:428	However, there is still no report for the comprehensive glycan profile of the respiratory tract tissues in tree shrews.					
32129075	2	45	theme	tree	418:421	arg1	shrews					423:428	tree shrews	418:428	tree shrews	418:428	However, there is still no report for the comprehensive glycan profile of the respiratory tract tissues in tree shrews.					
32129075	5	46	theme	run-to-run	826:835	arg1	variation					837:845	the inherent run-to-run variation	813:845	the inherent run-to-run variation in MS detection	813:861	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	3	47	theme	tree	529:532	arg1	shrews					534:539	tree shrews	529:539	tree shrews	529:539	In this study, we characterized the structural diversity of N-glycans in the respiratory tract of tree shrews using our well-established TiO2-PGC chip-Q-TOF-MS method.					
32129075	8	48	theme	tract	1236:1240	arg1	profile					1209:1215	the comprehensive glycomic profile	1182:1215	the comprehensive glycomic profile of the respiratory tract of tree shrews	1182:1255	This study is the first to reveal the comprehensive glycomic profile of the respiratory tract of tree shrews.					
32129075	0	49	theme	Tissues	54:60	arg1	Profiling					23:31	Comprehensive Glycomic Profiling	0:31	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.	0:111	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.					
32129075	1	50	theme	human	278:282	arg1	diseases					301:308	human viral infectious diseases	278:308	human viral infectious diseases	278:308	Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases.					
32129075	3	51	theme	shrews	534:539	arg1	tract					520:524	the respiratory tract	504:524	the respiratory tract of tree shrews	504:539	In this study, we characterized the structural diversity of N-glycans in the respiratory tract of tree shrews using our well-established TiO2-PGC chip-Q-TOF-MS method.					
32129075	8	52	theme	respiratory	1224:1234	arg1	tract					1236:1240	the respiratory tract	1220:1240	the respiratory tract of tree shrews	1220:1255	This study is the first to reveal the comprehensive glycomic profile of the respiratory tract of tree shrews.					
32129075	1	53	theme	viral	284:288	arg1	diseases					301:308	human viral infectious diseases	278:308	human viral infectious diseases	278:308	Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases.					
32129075	3	54	theme	chip-Q-TOF-MS	577:589	arg1	method					591:596	our well-established TiO2-PGC chip-Q-TOF-MS method	547:596	our well-established TiO2-PGC chip-Q-TOF-MS method	547:596	In this study, we characterized the structural diversity of N-glycans in the respiratory tract of tree shrews using our well-established TiO2-PGC chip-Q-TOF-MS method.					
32129075	4	55	theme	219	623:625	arg1	N-glycans					627:635	219 N-glycans	623:635	219 N-glycans	623:635	As a result, a total of 219 N-glycans were identified.					
32129075	8	56	theme	tree	1245:1248	arg1	shrews					1250:1255	tree shrews	1245:1255	tree shrews	1245:1255	This study is the first to reveal the comprehensive glycomic profile of the respiratory tract of tree shrews.					
32129075	9	57	theme	glycan	1313:1318	arg1	receptors					1320:1328	glycan receptors	1313:1328	glycan receptors	1313:1328	Our results also help to better understand the role of glycan receptors in human influenza infection and pathogenesis.					
32129075	6	58	theme	soft	976:979	arg1	palate					981:986	the soft palate	972:986	the soft palate	972:986	Our results showed that the N-glycan composition in the turbinate and lung was significantly different from the soft palate, trachea, and bronchus.					
32129075	5	59	used	used	797:800	arg2	standards					782:790	13C-labeled internal standards	761:790	13C-labeled internal standards	761:790	Moreover, each identified N-glycan was quantitated by a high sensitivity and accurate MRM method, in which 13C-labeled internal standards were used to correct the inherent run-to-run variation in MS detection.					
32129075	3	60	theme	structural	467:476	arg1	diversity					478:486	the structural diversity	463:486	the structural diversity of N-glycans in the respiratory tract of tree shrews	463:539	In this study, we characterized the structural diversity of N-glycans in the respiratory tract of tree shrews using our well-established TiO2-PGC chip-Q-TOF-MS method.					
32129075	9	61	theme	receptors	1320:1328	arg1	role					1305:1308	the role	1301:1308	the role of glycan receptors in human influenza infection and pathogenesis	1301:1374	Our results also help to better understand the role of glycan receptors in human influenza infection and pathogenesis.					
32129075	0	62	theme	Shrews	70:75	arg1	Tissues					54:60	Respiratory Tract Tissues	36:60	Respiratory Tract Tissues of Tree Shrews	36:75	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.					
32129075	3	63	theme	well-established	551:566	arg1	method					591:596	our well-established TiO2-PGC chip-Q-TOF-MS method	547:596	our well-established TiO2-PGC chip-Q-TOF-MS method	547:596	In this study, we characterized the structural diversity of N-glycans in the respiratory tract of tree shrews using our well-established TiO2-PGC chip-Q-TOF-MS method.					
32129075	1	64	theme	small	135:139	arg1	size					141:144	its relatively small size	120:144	its relatively small size	120:144	Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases.					
32129075	1	65	theme	tree	208:211	arg1	shrew					213:217	the tree shrew	204:217	the tree shrew	204:217	Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases.					
32129075	0	66	theme	Tree	65:68	arg1	Shrews					70:75	Tree Shrews	65:75	Tree Shrews	65:75	Comprehensive Glycomic Profiling of Respiratory Tract Tissues of Tree Shrews by TiO2-PGC Chip Mass Spectrometry.					
32129075	1	67	theme	infectious	290:299	arg1	diseases					301:308	human viral infectious diseases	278:308	human viral infectious diseases	278:308	Due to its relatively small size, homology to humans, and susceptibility to human viruses, the tree shrew becomes an ideal alternative animal model for the study of human viral infectious diseases.					
32129075	3	68	theme	TiO2-PGC	568:575	arg1	method					591:596	our well-established TiO2-PGC chip-Q-TOF-MS method	547:596	our well-established TiO2-PGC chip-Q-TOF-MS method	547:596	In this study, we characterized the structural diversity of N-glycans in the respiratory tract of tree shrews using our well-established TiO2-PGC chip-Q-TOF-MS method.					
32129075	2	69	theme	tissues	407:413	arg1	profile					374:380	the comprehensive glycan profile	349:380	the comprehensive glycan profile of the respiratory tract tissues in tree shrews	349:428	However, there is still no report for the comprehensive glycan profile of the respiratory tract tissues in tree shrews.					
32129075	3	70	from	diversity	478:486	arg1	tract					520:524	the respiratory tract	504:524	the respiratory tract of tree shrews	504:539	In this study, we characterized the structural diversity of N-glycans in the respiratory tract of tree shrews using our well-established TiO2-PGC chip-Q-TOF-MS method.					
32129075	9	71	theme	human	1333:1337	arg1	infection					1349:1357	human influenza infection	1333:1357	human influenza infection	1333:1357	Our results also help to better understand the role of glycan receptors in human influenza infection and pathogenesis.					
32485649	4	0	contain	containing	688:697	arg1	glycopeptides					674:686	glycopeptides	674:686	glycopeptides containing sialic acids	674:710	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	4	0	contain	containing	688:697	arg2	acids					706:710	sialic acids	699:710	sialic acids	699:710	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	1	1	from	separations	162:172	arg1	HILIC					224:228	HILIC	224:228	HILIC	224:228	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	1	1	from	separations	162:172	arg1	chromatography					208:221	hydrophilic interaction liquid chromatography	177:221	hydrophilic interaction liquid chromatography (HILIC)	177:229	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	1	2	theme	crucial	144:150	arg1	impact					152:157	a crucial impact	142:157	a crucial impact	142:157	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	0	3	from	precipitation	16:28	arg1	solvents					78:85	high organic content sample solvents	50:85	high organic content sample solvents	50:85	Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC.					
32485649	5	4	theme	adequate	898:905	arg1	separation					907:916	adequate separation	898:916	adequate separation	898:916	Therefore, the sample solvent composition in HILIC must be carefully optimized for accurate quantitative data collection and for adequate separation.					
32485649	3	5	theme	high	495:498	arg1	content					513:519	a high acetonitrile content	493:519	a high acetonitrile content	493:519	The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan.					
32485649	2	6	from	modifier	297:304	arg1	solvent					320:326	the sample solvent	309:326	the sample solvent	309:326	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	4	7	from	solubility	581:590	arg1	glycans					602:608	larger glycans	595:608	larger glycans attached to the same peptide backbone	595:646	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	3	8	theme	attached	544:551	arg1	N-glycan					553:560	attached N-glycan	544:560	attached N-glycan	544:560	The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan.					
32485649	3	9	theme	N-glycan	553:560	arg1	type					536:539	the type	532:539	the type of attached N-glycan	532:560	The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan.					
32485649	2	10	from	solvent	320:326	arg1	effect					276:281	the effect	272:281	the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins	272:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	4	11	theme	sialic	756:761	arg1	acid					763:766	sialic acid	756:766	sialic acid	756:766	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	4	12	theme	lower	575:579	arg1	solubility					581:590	lower solubility	575:590	lower solubility in larger glycans attached to the same peptide backbone	575:646	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	2	13	gly	glycopeptides	367:379	arg1	proteins					410:417	hemopexin and haptoglobin proteins	384:417	proteins	410:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	2	13	gly	glycopeptides	367:379	arg2	glycopeptides					367:379	different tryptic glycopeptides	349:379	different tryptic glycopeptides of hemopexin and haptoglobin proteins	349:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	2	14	theme	haptoglobin	398:408	arg1	proteins					410:417	hemopexin and haptoglobin proteins	384:417	proteins	410:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	5	15	theme	data	874:877	arg1	collection					879:888	accurate quantitative data collection	852:888	accurate quantitative data collection	852:888	Therefore, the sample solvent composition in HILIC must be carefully optimized for accurate quantitative data collection and for adequate separation.					
32485649	5	16	theme	accurate	852:859	arg1	collection					879:888	accurate quantitative data collection	852:888	accurate quantitative data collection	852:888	Therefore, the sample solvent composition in HILIC must be carefully optimized for accurate quantitative data collection and for adequate separation.					
32485649	5	17	theme	solvent	791:797	arg1	composition					799:809	the sample solvent composition	780:809	the sample solvent composition in HILIC	780:818	Therefore, the sample solvent composition in HILIC must be carefully optimized for accurate quantitative data collection and for adequate separation.					
32485649	5	18	from	composition	799:809	arg1	HILIC					814:818	HILIC	814:818	HILIC	814:818	Therefore, the sample solvent composition in HILIC must be carefully optimized for accurate quantitative data collection and for adequate separation.					
32485649	2	19	theme	glycopeptides	367:379	arg1	solubility					335:344	the solubility	331:344	the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins	331:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	1	20	contain	has	138:140	arg2	impact					152:157	a crucial impact	142:157	a crucial impact	142:157	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	1	20	contain	has	138:140	arg1	composition					106:116	The composition	102:116	The composition of a sample solvent	102:136	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	3	21	with	solvents	479:486	arg1	content					513:519	a high acetonitrile content	493:519	a high acetonitrile content	493:519	The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan.					
32485649	2	22	theme	modifier	297:304	arg1	effect					276:281	the effect	272:281	the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins	272:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	2	23	theme	tryptic	359:365	arg1	glycopeptides					367:379	different tryptic glycopeptides	349:379	different tryptic glycopeptides of hemopexin and haptoglobin proteins	349:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	0	24	theme	Glycan-specific	0:14	arg1	precipitation					16:28	Glycan-specific precipitation	0:28	Glycan-specific precipitation of glycopeptides in high organic content sample solvents	0:85	Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC.					
32485649	2	25	theme	organic	289:295	arg1	modifier					297:304	an organic modifier	286:304	an organic modifier in the sample solvent	286:326	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	1	26	theme	hydrophilic	177:187	arg1	HILIC					224:228	HILIC	224:228	HILIC	224:228	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	1	26	theme	hydrophilic	177:187	arg1	chromatography					208:221	hydrophilic interaction liquid chromatography	177:221	hydrophilic interaction liquid chromatography (HILIC)	177:229	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	0	27	theme	glycopeptides	33:45	arg1	precipitation					16:28	Glycan-specific precipitation	0:28	Glycan-specific precipitation of glycopeptides in high organic content sample solvents	0:85	Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC.					
32485649	4	28	attach	attached	610:617	arg2	glycans					602:608	larger glycans	595:608	larger glycans attached to the same peptide backbone	595:646	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	4	28	attach	attached	610:617	arg1	backbone					639:646	the same peptide backbone	622:646	the same peptide backbone	622:646	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	5	29	theme	sample	784:789	arg1	composition					799:809	the sample solvent composition	780:809	the sample solvent composition in HILIC	780:818	Therefore, the sample solvent composition in HILIC must be carefully optimized for accurate quantitative data collection and for adequate separation.					
32485649	1	30	theme	interaction	189:199	arg1	HILIC					224:228	HILIC	224:228	HILIC	224:228	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	1	30	theme	interaction	189:199	arg1	chromatography					208:221	hydrophilic interaction liquid chromatography	177:221	hydrophilic interaction liquid chromatography (HILIC)	177:229	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	0	31	used	used	87:90	arg2	precipitation					16:28	Glycan-specific precipitation	0:28	Glycan-specific precipitation of glycopeptides in high organic content sample solvents	0:85	Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC.					
32485649	3	32	gly	glycopeptides	462:474	arg2	glycopeptides					462:474	glycopeptides	462:474	glycopeptides	462:474	The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan.					
32485649	1	33	theme	liquid	201:206	arg1	HILIC					224:228	HILIC	224:228	HILIC	224:228	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	1	33	theme	liquid	201:206	arg1	chromatography					208:221	hydrophilic interaction liquid chromatography	177:221	hydrophilic interaction liquid chromatography (HILIC)	177:229	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	0	34	theme	high	50:53	arg1	solvents					78:85	high organic content sample solvents	50:85	high organic content sample solvents	50:85	Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC.					
32485649	4	35	theme	sialic	699:704	arg1	acids					706:710	sialic acids	699:710	sialic acids	699:710	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	2	36	theme	different	349:357	arg1	glycopeptides					367:379	different tryptic glycopeptides	349:379	different tryptic glycopeptides of hemopexin and haptoglobin proteins	349:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	4	37	theme	peptide	631:637	arg1	backbone					639:646	the same peptide backbone	622:646	the same peptide backbone	622:646	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	2	38	theme	hemopexin	384:392	arg1	proteins					410:417	hemopexin and haptoglobin proteins	384:417	proteins	410:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	0	39	theme	content	63:69	arg1	solvents					78:85	high organic content sample solvents	50:85	high organic content sample solvents	50:85	Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC.					
32485649	0	40	theme	organic	55:61	arg1	solvents					78:85	high organic content sample solvents	50:85	high organic content sample solvents	50:85	Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC.					
32485649	0	41	gly	glycopeptides	33:45	arg2	glycopeptides					33:45	glycopeptides	33:45	glycopeptides	33:45	Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC.					
32485649	3	42	from	solubility	448:457	arg1	solvents					479:486	solvents	479:486	solvents with a high acetonitrile content	479:519	The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan.					
32485649	2	43	theme	sample	313:318	arg1	solvent					320:326	the sample solvent	309:326	the sample solvent	309:326	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	4	44	gly	glycopeptides	674:686	arg2	glycopeptides					674:686	glycopeptides	674:686	glycopeptides containing sialic acids	674:710	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	1	45	theme	sample	123:128	arg1	solvent					130:136	a sample solvent	121:136	a sample solvent	121:136	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	4	46	theme	larger	595:600	arg1	glycans					602:608	larger glycans	595:608	larger glycans attached to the same peptide backbone	595:646	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	1	47	theme	solvent	130:136	arg1	composition					106:116	The composition	102:116	The composition of a sample solvent	102:136	The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC).					
32485649	2	48	from	effect	276:281	arg1	solvent					320:326	the sample solvent	309:326	the sample solvent	309:326	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	2	48	from	effect	276:281	arg1	solubility					335:344	the solubility	331:344	the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins	331:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	3	49	theme	glycopeptides	462:474	arg1	solubility					448:457	the solubility	444:457	the solubility of glycopeptides in solvents with a high acetonitrile content	444:519	The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan.					
32485649	4	50	theme	same	626:629	arg1	backbone					639:646	the same peptide backbone	622:646	the same peptide backbone	622:646	We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid.					
32485649	2	51	theme	short	240:244	arg1	communication					246:258	this short communication	235:258	this short communication	235:258	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	2	52	theme	proteins	410:417	arg1	glycopeptides					367:379	different tryptic glycopeptides	349:379	different tryptic glycopeptides of hemopexin and haptoglobin proteins	349:417	In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins.					
32485649	3	53	theme	acetonitrile	500:511	arg1	content					513:519	a high acetonitrile content	493:519	a high acetonitrile content	493:519	The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan.					
32485649	5	54	theme	quantitative	861:872	arg1	collection					879:888	accurate quantitative data collection	852:888	accurate quantitative data collection	852:888	Therefore, the sample solvent composition in HILIC must be carefully optimized for accurate quantitative data collection and for adequate separation.					
32485649	0	55	theme	sample	71:76	arg1	solvents					78:85	high organic content sample solvents	50:85	high organic content sample solvents	50:85	Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC.					
33735360	6	0	theme	different	957:965	arg1	methods					982:988	Two different derivatization methods	953:988	Two different derivatization methods	953:988	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	0	theme	different	957:965	arg1	esterification					1068:1081	linkage-specific sialic acid esterification	1039:1081	linkage-specific sialic acid esterification	1039:1081	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	0	theme	different	957:965	arg1	labeling					1026:1033	2-aminobenzoic acid (2-AA) labeling	999:1033	2-aminobenzoic acid (2-AA) labeling	999:1033	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	4	1	theme	differential	592:603	arg1	profiling					621:629	differential N-glycosylation profiling	592:629	differential N-glycosylation profiling of IDC cancer tissues	592:651	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	2	2	theme	research	331:338	arg1	point					315:319	a focal point	307:319	a focal point of cancer research involving predictive and prognostic markers	307:382	As dynamic changes of the glycome are closely associated with complex diseases, they have become a focal point of cancer research involving predictive and prognostic markers.					
33735360	3	3	theme	biomarker	552:560	arg1	discovery					562:570	biomarker discovery	552:570	biomarker discovery	552:570	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	4	4	from	studies	581:587	arg1	profiling					621:629	differential N-glycosylation profiling	592:629	differential N-glycosylation profiling of IDC cancer tissues	592:651	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	5	5	theme	spectrometry	837:848	arg1	analyses					867:874	matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses	788:874	matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses	788:874	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	1	6	theme	common	180:185	arg1	type					187:190	the most common type	171:190	the most common type of breast cancer	171:207	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	1	6	theme	common	180:185	arg1	carcinoma					152:160	Invasive ductal carcinoma	136:160	Invasive ductal carcinoma (IDC)	136:166	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	4	7	theme	biological	694:703	arg1	role					705:708	the biological role	690:708	the biological role of glycans in cancer	690:729	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	5	8	theme	differential	907:918	arg1	patterns					936:943	differential N-glycosylation patterns	907:943	differential N-glycosylation patterns of IDC	907:950	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	1	9	theme	cancer	202:207	arg1	type					187:190	the most common type	171:190	the most common type of breast cancer	171:207	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	1	9	theme	cancer	202:207	arg1	carcinoma					152:160	Invasive ductal carcinoma	136:160	Invasive ductal carcinoma (IDC)	136:166	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	6	10	theme	2-aminobenzoic	999:1012	arg1	acid					1014:1017	2-aminobenzoic acid	999:1017	2-aminobenzoic acid (2-AA) labeling	999:1033	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	10	theme	2-aminobenzoic	999:1012	arg1	2-AA					1020:1023	2-AA	1020:1023	2-AA	1020:1023	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	9	11	theme	fucosylated	1705:1715	arg1	H3N3F1					1731:1736	H3N3F1	1731:1736	H3N3F1	1731:1736	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	11	theme	fucosylated	1705:1715	arg1	H5N2					1675:1678	H5N2	1675:1678	H5N2	1675:1678	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	11	theme	fucosylated	1705:1715	arg1	H5N5F1					1742:1747	H5N5F1	1742:1747	H5N5F1	1742:1747	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	11	theme	fucosylated	1705:1715	arg1	H6N2					1681:1684	H6N2	1681:1684	H6N2	1681:1684	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	11	theme	fucosylated	1705:1715	arg1	compositions					1717:1728	two fucosylated compositions	1701:1728	two fucosylated compositions (H3N3F1 and H5N5F1)	1701:1748	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	11	theme	fucosylated	1705:1715	arg1	H7N2					1691:1694	H7N2	1691:1694	H7N2	1691:1694	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	8	12	theme	N-glycan	1336:1343	arg1	traits					1345:1350	15 N-glycan traits	1333:1350	15 N-glycan traits	1333:1350	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	2	13	theme	dynamic	213:219	arg1	changes					221:227	dynamic changes	213:227	dynamic changes of the glycome	213:242	As dynamic changes of the glycome are closely associated with complex diseases, they have become a focal point of cancer research involving predictive and prognostic markers.					
33735360	1	14	theme	ductal	145:150	arg1	carcinoma					152:160	Invasive ductal carcinoma	136:160	Invasive ductal carcinoma (IDC)	136:166	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	1	14	theme	ductal	145:150	arg1	type					187:190	the most common type	171:190	the most common type of breast cancer	171:207	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	1	14	theme	ductal	145:150	arg1	IDC					163:165	IDC	163:165	IDC	163:165	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	9	15	dep	compositions	1717:1728	arg1	H3N3F1					1731:1736	H3N3F1	1731:1736	H3N3F1	1731:1736	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	15	dep	compositions	1717:1728	arg1	H5N2					1675:1678	H5N2	1675:1678	H5N2	1675:1678	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	15	dep	compositions	1717:1728	arg1	H5N5F1					1742:1747	H5N5F1	1742:1747	H5N5F1	1742:1747	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	15	dep	compositions	1717:1728	arg1	H6N2					1681:1684	H6N2	1681:1684	H6N2	1681:1684	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	15	dep	compositions	1717:1728	arg1	compositions					1717:1728	two fucosylated compositions	1701:1728	two fucosylated compositions (H3N3F1 and H5N5F1)	1701:1748	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	15	dep	compositions	1717:1728	arg1	H7N2					1691:1694	H7N2	1691:1694	H7N2	1691:1694	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	2	16	theme	glycome	236:242	arg1	changes					221:227	dynamic changes	213:227	dynamic changes of the glycome	213:242	As dynamic changes of the glycome are closely associated with complex diseases, they have become a focal point of cancer research involving predictive and prognostic markers.					
33735360	6	17	theme	sialic	1056:1061	arg1	methods					982:988	Two different derivatization methods	953:988	Two different derivatization methods	953:988	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	17	theme	sialic	1056:1061	arg1	esterification					1068:1081	linkage-specific sialic acid esterification	1039:1081	linkage-specific sialic acid esterification	1039:1081	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	9	18	theme	strong	1757:1762	arg1	discrimination					1764:1777	strong discrimination	1757:1777	strong discrimination between IDC and controls	1757:1802	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	0	19	theme	ductal	91:96	arg1	tissues					108:114	formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	42:114	formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	42:114	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues using MALDI-TOF-MS.					
33735360	5	20	theme	IDC	948:950	arg1	patterns					936:943	differential N-glycosylation patterns	907:943	differential N-glycosylation patterns of IDC	907:950	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	8	21	theme	N-glycan	1544:1551	arg1	traits					1553:1558	17 N-glycan traits	1541:1558	17 N-glycan traits	1541:1558	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	0	22	theme	tissues	108:114	arg1	profiling					29:37	Differential N-glycosylation profiling	0:37	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	0:114	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues using MALDI-TOF-MS.					
33735360	3	23	dep	paraffin-embedded	400:416	arg1	FFPE					419:422	FFPE	419:422	FFPE	419:422	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	6	24	theme	N-glycans	1114:1122	arg1	analysis					1102:1109	the analysis	1098:1109	the analysis of N-glycans	1098:1122	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	4	25	theme	predictive	753:762	arg1	ability					764:770	their predictive ability	747:770	their predictive ability	747:770	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	3	26	theme	clinical	425:432	arg1	specimens					434:442	Formalin-fixed paraffin-embedded (FFPE) clinical specimens	385:442	Formalin-fixed paraffin-embedded (FFPE) clinical specimens	385:442	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	0	27	theme	Differential	0:11	arg1	profiling					29:37	Differential N-glycosylation profiling	0:37	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	0:114	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues using MALDI-TOF-MS.					
33735360	3	28	theme	tumor	470:474	arg1	environment					476:486	the tumor environment	466:486	the tumor environment	466:486	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	3	29	theme	related	531:537	arg1	research					539:546	cancer related research	524:546	cancer related research	524:546	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	4	30	theme	Further	573:579	arg1	studies					581:587	Further studies	573:587	Further studies on differential N-glycosylation profiling of IDC cancer tissues	573:651	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	10	31	theme	mannose	1851:1857	arg1	N-glycans					1859:1867	high mannose N-glycans	1846:1867	high mannose N-glycans	1846:1867	In addition, compared with the controls, high mannose N-glycans were observed to be up-regulated in IDC whereas bisecting N-glycans were down-regulated.					
33735360	8	32	theme	such	1436:1439	arg1	analyses					1441:1448	such analyses	1436:1448	such analyses for the ethyl-esterified N-glycans	1436:1483	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	0	33	theme	formalin-fixed	42:55	arg1	tissues					108:114	formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	42:114	formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	42:114	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues using MALDI-TOF-MS.					
33735360	5	34	theme	assisted	795:802	arg1	spectrometry					837:848	matrix assisted laser desorption ionization-mass spectrometry	788:848	matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses	788:874	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	5	34	theme	assisted	795:802	arg1	MALDI-MS					851:858	MALDI-MS	851:858	MALDI-MS	851:858	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	3	35	used	utilized	501:508	arg2	specimens					434:442	Formalin-fixed paraffin-embedded (FFPE) clinical specimens	385:442	Formalin-fixed paraffin-embedded (FFPE) clinical specimens	385:442	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	2	36	theme	cancer	324:329	arg1	research					331:338	cancer research	324:338	cancer research involving predictive and prognostic markers	324:382	As dynamic changes of the glycome are closely associated with complex diseases, they have become a focal point of cancer research involving predictive and prognostic markers.					
33735360	5	37	theme	desorption	810:819	arg1	spectrometry					837:848	matrix assisted laser desorption ionization-mass spectrometry	788:848	matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses	788:874	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	5	37	theme	desorption	810:819	arg1	MALDI-MS					851:858	MALDI-MS	851:858	MALDI-MS	851:858	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	0	38	dep	formalin-fixed	42:55	arg1	paraffin-embedded					57:73	paraffin-embedded	57:73	paraffin-embedded	57:73	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues using MALDI-TOF-MS.					
33735360	8	39	theme	ethyl-esterified	1458:1473	arg1	N-glycans					1475:1483	the ethyl-esterified N-glycans	1454:1483	the ethyl-esterified N-glycans	1454:1483	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	40	dep	significant	1359:1369	arg1	<					1374:1374	p < 0.05	1372:1379	p < 0.05	1372:1379	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	10	41	located	observed	1874:1881	arg1	addition					1808:1815	addition	1808:1815	addition	1808:1815	In addition, compared with the controls, high mannose N-glycans were observed to be up-regulated in IDC whereas bisecting N-glycans were down-regulated.					
33735360	10	41	located	observed	1874:1881	arg2	N-glycans					1859:1867	high mannose N-glycans	1846:1867	high mannose N-glycans	1846:1867	In addition, compared with the controls, high mannose N-glycans were observed to be up-regulated in IDC whereas bisecting N-glycans were down-regulated.					
33735360	8	42	dep	significant	1567:1577	arg1	<					1582:1582	p < 0.05	1580:1587	p < 0.05	1580:1587	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	6	43	theme	derivatization	967:980	arg1	methods					982:988	Two different derivatization methods	953:988	Two different derivatization methods	953:988	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	43	theme	derivatization	967:980	arg1	esterification					1068:1081	linkage-specific sialic acid esterification	1039:1081	linkage-specific sialic acid esterification	1039:1081	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	43	theme	derivatization	967:980	arg1	labeling					1026:1033	2-aminobenzoic acid (2-AA) labeling	999:1033	2-aminobenzoic acid (2-AA) labeling	999:1033	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	4	44	theme	cancer	638:643	arg1	tissues					645:651	IDC cancer tissues	634:651	IDC cancer tissues	634:651	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	7	45	theme	esterified	1166:1175	arg1	N-glycans					1177:1185	Forty-seven 2-AA labeled and fifty ethyl esterified N-glycans	1125:1185	Forty-seven 2-AA labeled and fifty ethyl esterified N-glycans	1125:1185	Forty-seven 2-AA labeled and fifty ethyl esterified N-glycans were identified by MALDI-MS.					
33735360	3	46	from	studies	513:519	arg1	research					539:546	cancer related research	524:546	cancer related research	524:546	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	3	46	from	studies	513:519	arg1	discovery					562:570	biomarker discovery	552:570	biomarker discovery	552:570	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	4	47	from	role	705:708	arg1	cancer					724:729	cancer	724:729	cancer	724:729	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	4	48	theme	N-glycosylation	605:619	arg1	profiling					621:629	differential N-glycosylation profiling	592:629	differential N-glycosylation profiling of IDC cancer tissues	592:651	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	7	49	theme	labeled	1142:1148	arg1	N-glycans					1177:1185	Forty-seven 2-AA labeled and fifty ethyl esterified N-glycans	1125:1185	Forty-seven 2-AA labeled and fifty ethyl esterified N-glycans	1125:1185	Forty-seven 2-AA labeled and fifty ethyl esterified N-glycans were identified by MALDI-MS.					
33735360	2	50	theme	complex	272:278	arg1	diseases					280:287	complex diseases	272:287	complex diseases	272:287	As dynamic changes of the glycome are closely associated with complex diseases, they have become a focal point of cancer research involving predictive and prognostic markers.					
33735360	8	51	theme	statistical	1219:1229	arg1	analyses					1231:1238	statistical analyses	1219:1238	statistical analyses conducted for 2-AA-labeled N-glycans	1219:1275	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	10	52	theme	bisecting	1917:1925	arg1	N-glycans					1927:1935	bisecting N-glycans	1917:1935	bisecting N-glycans	1917:1935	In addition, compared with the controls, high mannose N-glycans were observed to be up-regulated in IDC whereas bisecting N-glycans were down-regulated.					
33735360	9	53	gly	fucosylated	1705:1715	arg1	H3N3F1					1731:1736	H3N3F1	1731:1736	H3N3F1	1731:1736	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	53	gly	fucosylated	1705:1715	arg1	H5N2					1675:1678	H5N2	1675:1678	H5N2	1675:1678	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	53	gly	fucosylated	1705:1715	arg1	H5N5F1					1742:1747	H5N5F1	1742:1747	H5N5F1	1742:1747	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	53	gly	fucosylated	1705:1715	arg1	H6N2					1681:1684	H6N2	1681:1684	H6N2	1681:1684	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	53	gly	fucosylated	1705:1715	arg1	compositions					1717:1728	two fucosylated compositions	1701:1728	two fucosylated compositions (H3N3F1 and H5N5F1)	1701:1748	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	53	gly	fucosylated	1705:1715	arg1	H7N2					1691:1694	H7N2	1691:1694	H7N2	1691:1694	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	8	54	theme	N-glycans	1305:1313	arg1	N-glycans					1305:1313	32 N-glycans	1302:1313	32 N-glycans	1302:1313	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	54	theme	N-glycans	1305:1313	arg1	prevalence					1319:1328	prevalence	1319:1328	prevalence	1319:1328	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	54	theme	N-glycans	1305:1313	arg1	amounts					1291:1297	the relative amounts	1278:1297	the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits	1278:1350	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	7	55	dep	esterified	1166:1175	arg1	ethyl					1160:1164	fifty ethyl	1154:1164	fifty ethyl	1154:1164	Forty-seven 2-AA labeled and fifty ethyl esterified N-glycans were identified by MALDI-MS.					
33735360	1	56	theme	breast	195:200	arg1	cancer					202:207	breast cancer	195:207	breast cancer	195:207	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	8	57	theme	2-AA-labeled	1254:1265	arg1	N-glycans					1267:1275	2-AA-labeled N-glycans	1254:1275	2-AA-labeled N-glycans	1254:1275	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	6	58	theme	acid	1014:1017	arg1	methods					982:988	Two different derivatization methods	953:988	Two different derivatization methods	953:988	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	58	theme	acid	1014:1017	arg1	labeling					1026:1033	2-aminobenzoic acid (2-AA) labeling	999:1033	2-aminobenzoic acid (2-AA) labeling	999:1033	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	8	59	theme	relative	1490:1497	arg1	amounts					1499:1505	the relative amounts	1486:1505	the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits	1486:1558	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	59	theme	relative	1490:1497	arg1	N-glycans					1513:1521	27 N-glycans	1510:1521	27 N-glycans	1510:1521	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	59	theme	relative	1490:1497	arg1	prevalence					1527:1536	prevalence	1527:1536	prevalence	1527:1536	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	60	theme	prevalence	1319:1328	arg1	N-glycans					1305:1313	32 N-glycans	1302:1313	32 N-glycans	1302:1313	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	60	theme	prevalence	1319:1328	arg1	prevalence					1319:1328	prevalence	1319:1328	prevalence	1319:1328	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	60	theme	prevalence	1319:1328	arg1	amounts					1291:1297	the relative amounts	1278:1297	the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits	1278:1350	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	7	61	dep	labeled	1142:1148	arg1	2-AA					1137:1140	Forty-seven 2-AA	1125:1140	Forty-seven 2-AA	1125:1140	Forty-seven 2-AA labeled and fifty ethyl esterified N-glycans were identified by MALDI-MS.					
33735360	1	62	theme	Invasive	136:143	arg1	carcinoma					152:160	Invasive ductal carcinoma	136:160	Invasive ductal carcinoma (IDC)	136:166	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	1	62	theme	Invasive	136:143	arg1	type					187:190	the most common type	171:190	the most common type of breast cancer	171:207	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	1	62	theme	Invasive	136:143	arg1	IDC					163:165	IDC	163:165	IDC	163:165	Invasive ductal carcinoma (IDC) is the most common type of breast cancer.					
33735360	8	63	theme	relative	1282:1289	arg1	N-glycans					1305:1313	32 N-glycans	1302:1313	32 N-glycans	1302:1313	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	63	theme	relative	1282:1289	arg1	prevalence					1319:1328	prevalence	1319:1328	prevalence	1319:1328	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	63	theme	relative	1282:1289	arg1	amounts					1291:1297	the relative amounts	1278:1297	the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits	1278:1350	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	5	64	gly	N-glycosylation	920:934	arg1	IDC					948:950	IDC	948:950	IDC	948:950	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	8	65	theme	N-glycans	1513:1521	arg1	amounts					1499:1505	the relative amounts	1486:1505	the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits	1486:1558	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	65	theme	N-glycans	1513:1521	arg1	N-glycans					1513:1521	27 N-glycans	1510:1521	27 N-glycans	1510:1521	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	65	theme	N-glycans	1513:1521	arg1	prevalence					1527:1536	prevalence	1527:1536	prevalence	1527:1536	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	66	theme	traits	1345:1350	arg1	N-glycans					1305:1313	32 N-glycans	1302:1313	32 N-glycans	1302:1313	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	66	theme	traits	1345:1350	arg1	prevalence					1319:1328	prevalence	1319:1328	prevalence	1319:1328	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	67	theme	prevalence	1527:1536	arg1	amounts					1499:1505	the relative amounts	1486:1505	the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits	1486:1558	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	67	theme	prevalence	1527:1536	arg1	N-glycans					1513:1521	27 N-glycans	1510:1521	27 N-glycans	1510:1521	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	67	theme	prevalence	1527:1536	arg1	prevalence					1527:1536	prevalence	1527:1536	prevalence	1527:1536	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	68	theme	significant	1359:1369	arg1	differences					1382:1392	significant (p < 0.05) differences	1359:1392	significant (p < 0.05) differences between cancer and normal tissues	1359:1426	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	0	69	theme	invasive	82:89	arg1	tissues					108:114	formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	42:114	formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	42:114	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues using MALDI-TOF-MS.					
33735360	5	70	theme	N-glycosylation	920:934	arg1	patterns					936:943	differential N-glycosylation patterns	907:943	differential N-glycosylation patterns of IDC	907:950	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	6	71	theme	acid	1063:1066	arg1	methods					982:988	Two different derivatization methods	953:988	Two different derivatization methods	953:988	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	71	theme	acid	1063:1066	arg1	esterification					1068:1081	linkage-specific sialic acid esterification	1039:1081	linkage-specific sialic acid esterification	1039:1081	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	8	72	theme	p	1372:1372	arg1	<					1374:1374	p < 0.05	1372:1379	p < 0.05	1372:1379	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	0	73	theme	carcinoma	98:106	arg1	tissues					108:114	formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	42:114	formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	42:114	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues using MALDI-TOF-MS.					
33735360	6	74	theme	linkage-specific	1039:1054	arg1	methods					982:988	Two different derivatization methods	953:988	Two different derivatization methods	953:988	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	74	theme	linkage-specific	1039:1054	arg1	esterification					1068:1081	linkage-specific sialic acid esterification	1039:1081	linkage-specific sialic acid esterification	1039:1081	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	8	75	theme	traits	1553:1558	arg1	N-glycans					1513:1521	27 N-glycans	1510:1521	27 N-glycans	1510:1521	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	75	theme	traits	1553:1558	arg1	prevalence					1527:1536	prevalence	1527:1536	prevalence	1527:1536	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	4	76	theme	glycans	713:719	arg1	role					705:708	the biological role	690:708	the biological role of glycans in cancer	690:729	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	9	77	theme	mannose	1646:1652	arg1	H7N2					1691:1694	H7N2	1691:1694	H7N2	1691:1694	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	77	theme	mannose	1646:1652	arg1	H6N2					1681:1684	H6N2	1681:1684	H6N2	1681:1684	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	77	theme	mannose	1646:1652	arg1	N-glycans					1654:1662	mainly high mannose N-glycans	1634:1662	mainly high mannose N-glycans	1634:1662	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	77	theme	mannose	1646:1652	arg1	H5N2					1675:1678	H5N2	1675:1678	H5N2	1675:1678	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	6	78	used	used	1089:1092	arg2	methods					982:988	Two different derivatization methods	953:988	Two different derivatization methods	953:988	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	78	used	used	1089:1092	arg2	esterification					1068:1081	linkage-specific sialic acid esterification	1039:1081	linkage-specific sialic acid esterification	1039:1081	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	6	78	used	used	1089:1092	arg2	labeling					1026:1033	2-aminobenzoic acid (2-AA) labeling	999:1033	2-aminobenzoic acid (2-AA) labeling	999:1033	Two different derivatization methods, namely, 2-aminobenzoic acid (2-AA) labeling and linkage-specific sialic acid esterification, were used for the analysis of N-glycans.					
33735360	8	79	theme	significant	1567:1577	arg1	differences					1590:1600	significant (p < 0.05) differences	1567:1600	significant (p < 0.05) differences between them	1567:1613	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	3	80	dep	Formalin-fixed	385:398	arg1	paraffin-embedded					400:416	paraffin-embedded	400:416	paraffin-embedded	400:416	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	2	81	theme	focal	309:313	arg1	point					315:319	a focal point	307:319	a focal point of cancer research involving predictive and prognostic markers	307:382	As dynamic changes of the glycome are closely associated with complex diseases, they have become a focal point of cancer research involving predictive and prognostic markers.					
33735360	5	82	theme	-based	860:865	arg1	analyses					867:874	matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses	788:874	matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses	788:874	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	8	83	theme	p	1580:1580	arg1	<					1582:1582	p < 0.05	1580:1587	p < 0.05	1580:1587	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	8	84	theme	cancer	1402:1407	arg1	tissues					1420:1426	cancer and normal tissues	1402:1426	tissues	1420:1426	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	5	85	theme	ionization-mass	821:835	arg1	spectrometry					837:848	matrix assisted laser desorption ionization-mass spectrometry	788:848	matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses	788:874	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	5	85	theme	ionization-mass	821:835	arg1	MALDI-MS					851:858	MALDI-MS	851:858	MALDI-MS	851:858	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	3	86	theme	Formalin-fixed	385:398	arg1	specimens					434:442	Formalin-fixed paraffin-embedded (FFPE) clinical specimens	385:442	Formalin-fixed paraffin-embedded (FFPE) clinical specimens	385:442	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	8	87	theme	normal	1413:1418	arg1	tissues					1420:1426	cancer and normal tissues	1402:1426	tissues	1420:1426	In statistical analyses conducted for 2-AA-labeled N-glycans, the relative amounts of 32 N-glycans and prevalence of 15 N-glycan traits showed significant (p < 0.05) differences between cancer and normal tissues; and in such analyses for the ethyl-esterified N-glycans, the relative amounts of 27 N-glycans and prevalence of 17 N-glycan traits showed significant (p < 0.05) differences between them.					
33735360	2	88	theme	prognostic	365:374	arg1	markers					376:382	predictive and prognostic markers	350:382	predictive and prognostic markers	350:382	As dynamic changes of the glycome are closely associated with complex diseases, they have become a focal point of cancer research involving predictive and prognostic markers.					
33735360	3	89	theme	environment	476:486	arg1	representative					448:461	representative	448:461	representative	448:461	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	5	90	theme	matrix	788:793	arg1	spectrometry					837:848	matrix assisted laser desorption ionization-mass spectrometry	788:848	matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses	788:874	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	5	90	theme	matrix	788:793	arg1	MALDI-MS					851:858	MALDI-MS	851:858	MALDI-MS	851:858	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	10	91	theme	high	1846:1849	arg1	N-glycans					1859:1867	high mannose N-glycans	1846:1867	high mannose N-glycans	1846:1867	In addition, compared with the controls, high mannose N-glycans were observed to be up-regulated in IDC whereas bisecting N-glycans were down-regulated.					
33735360	5	92	theme	laser	804:808	arg1	spectrometry					837:848	matrix assisted laser desorption ionization-mass spectrometry	788:848	matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses	788:874	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	5	92	theme	laser	804:808	arg1	MALDI-MS					851:858	MALDI-MS	851:858	MALDI-MS	851:858	In this study, matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)-based analyses were conducted for determining differential N-glycosylation patterns of IDC.					
33735360	0	93	dep	paraffin-embedded	57:73	arg1	FFPE					76:79	FFPE	76:79	FFPE	76:79	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues using MALDI-TOF-MS.					
33735360	2	94	theme	predictive	350:359	arg1	markers					376:382	predictive and prognostic markers	350:382	predictive and prognostic markers	350:382	As dynamic changes of the glycome are closely associated with complex diseases, they have become a focal point of cancer research involving predictive and prognostic markers.					
33735360	9	95	theme	high	1641:1644	arg1	H7N2					1691:1694	H7N2	1691:1694	H7N2	1691:1694	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	95	theme	high	1641:1644	arg1	H6N2					1681:1684	H6N2	1681:1684	H6N2	1681:1684	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	95	theme	high	1641:1644	arg1	N-glycans					1654:1662	mainly high mannose N-glycans	1634:1662	mainly high mannose N-glycans	1634:1662	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	9	95	theme	high	1641:1644	arg1	H5N2					1675:1678	H5N2	1675:1678	H5N2	1675:1678	It was found that mainly high mannose N-glycans, including H5N2, H6N2, and H7N2, and two fucosylated compositions (H3N3F1 and H5N5F1) showed strong discrimination between IDC and controls.					
33735360	4	96	theme	IDC	634:636	arg1	tissues					645:651	IDC cancer tissues	634:651	IDC cancer tissues	634:651	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	3	97	theme	cancer	524:529	arg1	research					539:546	cancer related research	524:546	cancer related research	524:546	Formalin-fixed paraffin-embedded (FFPE) clinical specimens are representative of the tumor environment and are thus utilized in studies on cancer related research and biomarker discovery.					
33735360	4	98	theme	tissues	645:651	arg1	profiling					621:629	differential N-glycosylation profiling	592:629	differential N-glycosylation profiling of IDC cancer tissues	592:651	Further studies on differential N-glycosylation profiling of IDC cancer tissues are necessary in order to understand the biological role of glycans in cancer and to evaluate their predictive ability.					
33735360	0	99	theme	N-glycosylation	13:27	arg1	profiling					29:37	Differential N-glycosylation profiling	0:37	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues	0:114	Differential N-glycosylation profiling of formalin-fixed paraffin-embedded (FFPE) invasive ductal carcinoma tissues using MALDI-TOF-MS.					
32477333	14	0	theme	bLF	2351:2353	arg1	activation					2363:2372	bLF onTLR-8 activation	2351:2372	bLF onTLR-8 activation	2351:2372	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation may become a food-based strategy to manage autoimmune, infections or other inflammatory disorders.					
32477333	11	1	theme	immunomodulatory	1970:1985	arg1	activity					1987:1994	the immunomodulatory activity	1966:1994	the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs	1966:2038	This provides evidence that glycan composition plays a role in the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs.					
32477333	9	2	gly	desialylated	1695:1706	arg1	N-glycans					1731:1739	partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans	1633:1739	partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans	1633:1739	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	9	2	gly	desialylated	1695:1706	arg1	unmodified					1598:1607	unmodified	1598:1607	unmodified	1598:1607	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	3	3	from	impact	474:479	arg1	inhibition					539:548	the inhibition	535:548	the inhibition of TLR-8 activation	535:568	In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.					
32477333	9	4	theme	significant	1536:1546	arg1	decrease					1548:1555	a significant decrease	1534:1555	a significant decrease of IL-6	1534:1563	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	8	5	theme	inhibition	1292:1301	arg1	capacity					1303:1310	the inhibition capacity	1288:1310	the inhibition capacity of TLR-8	1288:1319	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	11	6	theme	isolated	2003:2010	arg1	N-glycans					2012:2020	the isolated N-glycans	1999:2020	the isolated N-glycans from bLF on MoDCs	1999:2038	This provides evidence that glycan composition plays a role in the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs.					
32477333	6	7	theme	TLR-	1083:1086	arg1	activation					1088:1097	endosomal TLR- activation	1073:1097	endosomal TLR- activation	1073:1097	The impact was compared with a pharmaceutical agent, i.e., chloroquine (CQN), that is clinically applied to antagonize endosomal TLR- activation.					
32477333	4	8	theme	native	621:626	arg1	form					628:631	their native form	615:631	their native form	615:631	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	3	9	theme	bovine	507:512	arg1	lactoferrin					514:524	bovine lactoferrin	507:524	bovine lactoferrin (bLF)	507:530	In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.					
32477333	3	9	theme	bovine	507:512	arg1	bLF					527:529	bLF	527:529	bLF	527:529	In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.					
32477333	12	10	from	inhibitors	2085:2094	arg1	MoDCs					2142:2146	MoDCs	2142:2146	MoDCs	2142:2146	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	14	11	theme	inhibitory	2304:2313	arg1	capacity					2315:2322	The inhibitory capacity	2300:2322	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation	2300:2372	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation may become a food-based strategy to manage autoimmune, infections or other inflammatory disorders.					
32477333	8	12	from	changes	1464:1470	arg1	endosome					1479:1486	the endosome	1475:1486	the endosome	1475:1486	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	13	13	theme	HEK293	2270:2275	arg1	cells					2277:2281	HEK293 cells	2270:2281	HEK293 cells	2270:2281	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	10	14	theme	desialylated	1798:1809	arg1	N-glycans					1811:1819	the partially demannosylated and partially desialylated N-glycans	1755:1819	the partially demannosylated and partially desialylated N-glycans	1755:1819	Furthermore, the partially demannosylated and partially desialylated N-glycans showed stronger inhibition of IL-6 production compared with the native N-glycans.					
32477333	10	15	theme	IL-6	1851:1854	arg1	production					1856:1865	IL-6 production	1851:1865	IL-6 production	1851:1865	Furthermore, the partially demannosylated and partially desialylated N-glycans showed stronger inhibition of IL-6 production compared with the native N-glycans.					
32477333	3	16	theme	activation	559:568	arg1	inhibition					539:548	the inhibition	535:548	the inhibition of TLR-8 activation	535:568	In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.					
32477333	1	17	theme	autoimmune	191:200	arg1	disorders					202:210	autoimmune disorders	191:210	autoimmune disorders	191:210	Toll-like receptor 8 (TLR-8) plays a role in the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients.					
32477333	9	18	dep	0.3-fold	1659:1666	arg1	p					1669:1669	p < 0.0001	1669:1678	p < 0.0001	1669:1678	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	13	19	from	activation	2256:2265	arg1	cells					2277:2281	HEK293 cells	2270:2281	HEK293 cells	2270:2281	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	13	19	from	activation	2256:2265	arg1	MoDCs					2293:2297	human MoDCs	2287:2297	human MoDCs	2287:2297	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	12	20	from	MoDCs	2142:2146	arg1	N-glycans					2062:2070	the N-glycans	2058:2070	the N-glycans	2058:2070	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	12	20	from	MoDCs	2142:2146	arg1	inhibitors					2085:2094	specific inhibitors	2076:2094	specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs	2076:2146	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	2	21	theme	spectrum	406:413	arg1	progression					368:378	progression	368:378	progression	368:378	Dietary interventions are becoming more accepted as mean to manage onset, progression, and treatment of a broad spectrum of inflammatory conditions.					
32477333	2	21	theme	spectrum	406:413	arg1	treatment					385:393	treatment	385:393	treatment	385:393	Dietary interventions are becoming more accepted as mean to manage onset, progression, and treatment of a broad spectrum of inflammatory conditions.					
32477333	2	21	theme	spectrum	406:413	arg1	onset					361:365	onset	361:365	onset	361:365	Dietary interventions are becoming more accepted as mean to manage onset, progression, and treatment of a broad spectrum of inflammatory conditions.					
32477333	14	22	theme	other	2443:2447	arg1	disorders					2462:2470	other inflammatory disorders	2443:2470	other inflammatory disorders	2443:2470	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation may become a food-based strategy to manage autoimmune, infections or other inflammatory disorders.					
32477333	12	23	theme	production	2128:2137	arg1	N-glycans					2062:2070	the N-glycans	2058:2070	the N-glycans	2058:2070	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	12	23	theme	production	2128:2137	arg1	inhibitors					2085:2094	specific inhibitors	2076:2094	specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs	2076:2146	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	12	24	from	production	2128:2137	arg1	MoDCs					2142:2146	MoDCs	2142:2146	MoDCs	2142:2146	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	9	25	dep	0.4-fold	1709:1716	arg1	<					1721:1721	p < 0.0001	1719:1728	p < 0.0001	1719:1728	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	8	26	theme	interaction	1407:1417	arg1	result					1388:1393	a result	1386:1393	a result of a direct interaction with the receptor rather than a result of pH changes in the endosome	1386:1486	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	8	26	theme	interaction	1407:1417	arg1	inhibition					1346:1355	the inhibition	1342:1355	the inhibition mediated by the N-glycans	1342:1381	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	4	27	theme	HEK293	713:718	arg1	cells					720:724	HEK293 cells	713:724	HEK293 cells expressing TLR-8	713:741	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	4	28	gly	desialylated	691:702	arg1	form					704:707	its partially demannosylated and partially desialylated form	648:707	its partially demannosylated and partially desialylated form	648:707	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	14	29	theme	autoimmune	2417:2426	arg1	infections					2429:2438	infections	2429:2438	infections	2429:2438	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation may become a food-based strategy to manage autoimmune, infections or other inflammatory disorders.					
32477333	9	30	theme	demannosylated	1643:1656	arg1	N-glycans					1731:1739	partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans	1633:1739	partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans	1633:1739	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	9	30	theme	demannosylated	1643:1656	arg1	unmodified					1598:1607	unmodified	1598:1607	unmodified	1598:1607	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	12	31	theme	activation	2105:2114	arg1	N-glycans					2062:2070	the N-glycans	2058:2070	the N-glycans	2058:2070	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	12	31	theme	activation	2105:2114	arg1	inhibitors					2085:2094	specific inhibitors	2076:2094	specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs	2076:2146	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	12	32	theme	TLR-8	2099:2103	arg1	activation					2105:2114	TLR-8 activation	2099:2114	TLR-8 activation	2099:2114	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	2	33	theme	conditions	431:440	arg1	spectrum					406:413	a broad spectrum	398:413	a broad spectrum of inflammatory conditions	398:440	Dietary interventions are becoming more accepted as mean to manage onset, progression, and treatment of a broad spectrum of inflammatory conditions.					
32477333	4	34	theme	desialylated	691:702	arg1	form					704:707	its partially demannosylated and partially desialylated form	648:707	its partially demannosylated and partially desialylated form	648:707	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	11	35	from	bLF	2027:2029	arg1	MoDCs					2034:2038	MoDCs	2034:2038	MoDCs	2034:2038	This provides evidence that glycan composition plays a role in the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs.					
32477333	11	35	from	bLF	2027:2029	arg1	activity					1987:1994	the immunomodulatory activity	1966:1994	the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs	1966:2038	This provides evidence that glycan composition plays a role in the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs.					
32477333	11	35	from	bLF	2027:2029	arg1	N-glycans					2012:2020	the isolated N-glycans	1999:2020	the isolated N-glycans from bLF on MoDCs	1999:2038	This provides evidence that glycan composition plays a role in the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs.					
32477333	0	36	theme	Inhibitory	0:9	arg1	Effects					11:17	Inhibitory Effects	0:17	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8	0:86	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.					
32477333	5	37	theme	TLR-8	879:883	arg1	activation					885:894	the ssRNA40 induced TLR-8 activation	859:894	the ssRNA40 induced TLR-8 activation	859:894	We found that in HEK293 cells, N-glycans strongly inhibited the ssRNA40 induced TLR-8 activation but to a lesser extent the R848 induced TLR-8 activation.					
32477333	9	38	from	inhibition	1493:1502	arg1	MoDCs					1516:1520	MoDCs	1516:1520	MoDCs	1516:1520	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	6	39	theme	pharmaceutical	985:998	arg1	agent					1000:1004	a pharmaceutical agent	983:1004	a pharmaceutical agent	983:1004	The impact was compared with a pharmaceutical agent, i.e., chloroquine (CQN), that is clinically applied to antagonize endosomal TLR- activation.					
32477333	6	40	dep	chloroquine	1013:1023	arg1	i.e.					1007:1010	i.e.	1007:1010	i.e.	1007:1010	The impact was compared with a pharmaceutical agent, i.e., chloroquine (CQN), that is clinically applied to antagonize endosomal TLR- activation.					
32477333	2	41	theme	broad	400:404	arg1	spectrum					406:413	a broad spectrum	398:413	a broad spectrum of inflammatory conditions	398:440	Dietary interventions are becoming more accepted as mean to manage onset, progression, and treatment of a broad spectrum of inflammatory conditions.					
32477333	10	42	gly	desialylated	1798:1809	arg1	N-glycans					1811:1819	the partially demannosylated and partially desialylated N-glycans	1755:1819	the partially demannosylated and partially desialylated N-glycans	1755:1819	Furthermore, the partially demannosylated and partially desialylated N-glycans showed stronger inhibition of IL-6 production compared with the native N-glycans.					
32477333	4	43	theme	monocyte-derived	757:772	arg1	MoDCs					791:795	MoDCs	791:795	MoDCs	791:795	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	4	43	theme	monocyte-derived	757:772	arg1	cells					784:788	human monocyte-derived dendritic cells	751:788	human monocyte-derived dendritic cells (MoDCs)	751:796	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	0	44	theme	N-Glycans	30:38	arg1	Effects					11:17	Inhibitory Effects	0:17	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8	0:86	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.					
32477333	13	45	theme	induced	2241:2247	arg1	activation					2256:2265	TLR-8 induced immune activation	2235:2265	TLR-8 induced immune activation in HEK293 cells and human MoDCs	2235:2297	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	3	46	attach	derived	494:500	arg1	lactoferrin					514:524	bovine lactoferrin	507:524	bovine lactoferrin (bLF)	507:530	In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.					
32477333	3	46	attach	derived	494:500	arg2	N-glycans					484:492	N-glycans	484:492	N-glycans derived from bovine lactoferrin (bLF)	484:530	In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.					
32477333	3	46	attach	derived	494:500	arg1	bLF					527:529	bLF	527:529	bLF	527:529	In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.					
32477333	8	47	theme	changes	1464:1470	arg1	result					1451:1456	a result	1449:1456	a result of a direct interaction with the receptor rather than a result of pH changes in the endosome	1386:1486	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	0	48	theme	Bovine	45:50	arg1	Lactoferrin					52:62	Bovine Lactoferrin	45:62	Bovine Lactoferrin	45:62	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.					
32477333	7	49	gly	desialylated	1201:1212	arg1	N-glycans					1214:1222	the partially demannosylated or partially desialylated N-glycans	1159:1222	the partially demannosylated or partially desialylated N-glycans	1159:1222	Inhibitory effects of the N-glycans were not influenced by the partially demannosylated or partially desialylated N-glycans.					
32477333	11	50	theme	N-glycans	2012:2020	arg1	activity					1987:1994	the immunomodulatory activity	1966:1994	the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs	1966:2038	This provides evidence that glycan composition plays a role in the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs.					
32477333	9	51	theme	desialylated	1695:1706	arg1	N-glycans					1731:1739	partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans	1633:1739	partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans	1633:1739	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	9	51	theme	desialylated	1695:1706	arg1	unmodified					1598:1607	unmodified	1598:1607	unmodified	1598:1607	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	4	52	theme	human	751:755	arg1	MoDCs					791:795	MoDCs	791:795	MoDCs	791:795	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	4	52	theme	human	751:755	arg1	cells					784:788	human monocyte-derived dendritic cells	751:788	human monocyte-derived dendritic cells (MoDCs)	751:796	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	1	53	theme	disorders	202:210	arg1	pathogenesis					175:186	the pathogenesis	171:186	the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients	171:291	Toll-like receptor 8 (TLR-8) plays a role in the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients.					
32477333	13	54	theme	attenuating	2212:2222	arg1	effects					2224:2230	attenuating effects	2212:2230	attenuating effects	2212:2230	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	13	55	from	bLF	2203:2205	arg1	N-glycans					2188:2196	isolated N-glycans	2179:2196	isolated N-glycans from bLF	2179:2205	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	3	56	theme	N-glycans	484:492	arg1	impact					474:479	the impact	470:479	the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation	470:568	In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.					
32477333	1	57	theme	associated	216:225	arg1	symptoms					244:251	associated gastrointestinal symptoms	216:251	associated gastrointestinal symptoms	216:251	Toll-like receptor 8 (TLR-8) plays a role in the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients.					
32477333	14	58	theme	onTLR-8	2355:2361	arg1	activation					2363:2372	bLF onTLR-8 activation	2351:2372	bLF onTLR-8 activation	2351:2372	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation may become a food-based strategy to manage autoimmune, infections or other inflammatory disorders.					
32477333	5	59	theme	TLR-8	936:940	arg1	activation					942:951	TLR-8 activation	936:951	TLR-8 activation	936:951	We found that in HEK293 cells, N-glycans strongly inhibited the ssRNA40 induced TLR-8 activation but to a lesser extent the R848 induced TLR-8 activation.					
32477333	7	60	theme	demannosylated	1173:1186	arg1	N-glycans					1214:1222	the partially demannosylated or partially desialylated N-glycans	1159:1222	the partially demannosylated or partially desialylated N-glycans	1159:1222	Inhibitory effects of the N-glycans were not influenced by the partially demannosylated or partially desialylated N-glycans.					
32477333	9	61	theme	p	1719:1719	arg1	<					1721:1721	p < 0.0001	1719:1728	p < 0.0001	1719:1728	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	1	62	theme	symptoms	244:251	arg1	pathogenesis					175:186	the pathogenesis	171:186	the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients	171:291	Toll-like receptor 8 (TLR-8) plays a role in the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients.					
32477333	8	63	from	result	1451:1456	arg1	endosome					1479:1486	the endosome	1475:1486	the endosome	1475:1486	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	14	64	theme	N-glycans	2327:2335	arg1	capacity					2315:2322	The inhibitory capacity	2300:2322	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation	2300:2372	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation may become a food-based strategy to manage autoimmune, infections or other inflammatory disorders.					
32477333	10	65	theme	demannosylated	1769:1782	arg1	N-glycans					1811:1819	the partially demannosylated and partially desialylated N-glycans	1755:1819	the partially demannosylated and partially desialylated N-glycans	1755:1819	Furthermore, the partially demannosylated and partially desialylated N-glycans showed stronger inhibition of IL-6 production compared with the native N-glycans.					
32477333	8	66	from	endosome	1479:1486	arg1	result					1451:1456	a result	1449:1456	a result of a direct interaction with the receptor rather than a result of pH changes in the endosome	1386:1486	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	13	67	theme	isolated	2179:2186	arg1	N-glycans					2188:2196	isolated N-glycans	2179:2196	isolated N-glycans from bLF	2179:2205	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	13	68	contain	have	2207:2210	arg1	N-glycans					2188:2196	isolated N-glycans	2179:2196	isolated N-glycans from bLF	2179:2205	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	13	68	contain	have	2207:2210	arg2	effects					2224:2230	attenuating effects	2212:2230	attenuating effects	2212:2230	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	9	69	theme	IL-6	1560:1563	arg1	decrease					1548:1555	a significant decrease	1534:1555	a significant decrease of IL-6	1534:1563	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	6	70	theme	endosomal	1073:1081	arg1	activation					1088:1097	endosomal TLR- activation	1073:1097	endosomal TLR- activation	1073:1097	The impact was compared with a pharmaceutical agent, i.e., chloroquine (CQN), that is clinically applied to antagonize endosomal TLR- activation.					
32477333	11	71	from	activity	1987:1994	arg1	bLF					2027:2029	bLF	2027:2029	bLF on MoDCs	2027:2038	This provides evidence that glycan composition plays a role in the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs.					
32477333	2	72	theme	Dietary	294:300	arg1	interventions					302:314	Dietary interventions	294:314	Dietary interventions	294:314	Dietary interventions are becoming more accepted as mean to manage onset, progression, and treatment of a broad spectrum of inflammatory conditions.					
32477333	8	73	from	difference	1232:1241	arg1	charge					1246:1251	charge	1246:1251	charge of the N-glycans	1246:1268	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	11	74	theme	glycan	1931:1936	arg1	composition					1938:1948	glycan composition	1931:1948	glycan composition	1931:1948	This provides evidence that glycan composition plays a role in the immunomodulatory activity of the isolated N-glycans from bLF on MoDCs.					
32477333	4	75	gly	demannosylated	662:675	arg1	form					704:707	its partially demannosylated and partially desialylated form	648:707	its partially demannosylated and partially desialylated form	648:707	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	3	76	theme	TLR-8	553:557	arg1	activation					559:568	TLR-8 activation	553:568	TLR-8 activation	553:568	In this study, we assessed the impact of N-glycans derived from bovine lactoferrin (bLF) on the inhibition of TLR-8 activation.					
32477333	4	77	from	effects	591:597	arg1	form					704:707	its partially demannosylated and partially desialylated form	648:707	its partially demannosylated and partially desialylated form	648:707	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	4	77	from	effects	591:597	arg1	cells					720:724	HEK293 cells	713:724	HEK293 cells expressing TLR-8	713:741	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	4	77	from	effects	591:597	arg1	form					628:631	their native form	615:631	their native form	615:631	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	4	77	from	effects	591:597	arg1	MoDCs					791:795	MoDCs	791:795	MoDCs	791:795	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	4	77	from	effects	591:597	arg1	cells					784:788	human monocyte-derived dendritic cells	751:788	human monocyte-derived dendritic cells (MoDCs)	751:796	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	4	78	theme	N-glycans	602:610	arg1	effects					591:597	the effects	587:597	the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs)	587:796	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	9	79	dep	demannosylated	1643:1656	arg1	0.4-fold					1709:1716	0.4-fold	1709:1716	0.4-fold	1709:1716	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	9	79	dep	demannosylated	1643:1656	arg1	0.3-fold					1659:1666	0.3-fold	1659:1666	0.3-fold	1659:1666	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	10	80	gly	demannosylated	1769:1782	arg1	N-glycans					1811:1819	the partially demannosylated and partially desialylated N-glycans	1755:1819	the partially demannosylated and partially desialylated N-glycans	1755:1819	Furthermore, the partially demannosylated and partially desialylated N-glycans showed stronger inhibition of IL-6 production compared with the native N-glycans.					
32477333	10	81	theme	stronger	1828:1835	arg1	inhibition					1837:1846	stronger inhibition	1828:1846	stronger inhibition of IL-6 production	1828:1865	Furthermore, the partially demannosylated and partially desialylated N-glycans showed stronger inhibition of IL-6 production compared with the native N-glycans.					
32477333	1	82	theme	Toll-like	126:134	arg1	receptor					136:143	Toll-like receptor 8	126:145	Toll-like receptor 8 (TLR-8)	126:153	Toll-like receptor 8 (TLR-8) plays a role in the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients.					
32477333	1	82	theme	Toll-like	126:134	arg1	TLR-8					148:152	TLR-8	148:152	TLR-8	148:152	Toll-like receptor 8 (TLR-8) plays a role in the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients.					
32477333	14	83	attach	isolated	2337:2344	arg1	activation					2363:2372	bLF onTLR-8 activation	2351:2372	bLF onTLR-8 activation	2351:2372	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation may become a food-based strategy to manage autoimmune, infections or other inflammatory disorders.					
32477333	14	83	attach	isolated	2337:2344	arg2	N-glycans					2327:2335	N-glycans	2327:2335	N-glycans isolated from bLF onTLR-8 activation	2327:2372	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation may become a food-based strategy to manage autoimmune, infections or other inflammatory disorders.					
32477333	4	84	theme	demannosylated	662:675	arg1	form					704:707	its partially demannosylated and partially desialylated form	648:707	its partially demannosylated and partially desialylated form	648:707	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	0	85	theme	Toll-Like	67:75	arg1	Receptor					77:84	Toll-Like Receptor 8	67:86	Toll-Like Receptor 8	67:86	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.					
32477333	5	86	theme	HEK293	816:821	arg1	cells					823:827	HEK293 cells	816:827	HEK293 cells	816:827	We found that in HEK293 cells, N-glycans strongly inhibited the ssRNA40 induced TLR-8 activation but to a lesser extent the R848 induced TLR-8 activation.					
32477333	14	87	theme	inflammatory	2449:2460	arg1	disorders					2462:2470	other inflammatory disorders	2443:2470	other inflammatory disorders	2443:2470	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation may become a food-based strategy to manage autoimmune, infections or other inflammatory disorders.					
32477333	9	88	theme	<	1622:1622	arg1	p					1620:1620	p < 0.0001	1620:1629	p < 0.0001	1620:1629	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	8	89	theme	direct	1400:1405	arg1	interaction					1407:1417	a direct interaction	1398:1417	a direct interaction with the receptor	1398:1435	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	10	90	theme	production	1856:1865	arg1	inhibition					1837:1846	stronger inhibition	1828:1846	stronger inhibition of IL-6 production	1828:1865	Furthermore, the partially demannosylated and partially desialylated N-glycans showed stronger inhibition of IL-6 production compared with the native N-glycans.					
32477333	7	91	theme	N-glycans	1126:1134	arg1	effects					1111:1117	Inhibitory effects	1100:1117	Inhibitory effects of the N-glycans	1100:1134	Inhibitory effects of the N-glycans were not influenced by the partially demannosylated or partially desialylated N-glycans.					
32477333	9	92	gly	demannosylated	1643:1656	arg1	N-glycans					1731:1739	partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans	1633:1739	partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans	1633:1739	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	9	92	gly	demannosylated	1643:1656	arg1	unmodified					1598:1607	unmodified	1598:1607	unmodified	1598:1607	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	0	93	from	Effects	11:17	arg1	Receptor					77:84	Toll-Like Receptor 8	67:86	Toll-Like Receptor 8	67:86	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.					
32477333	0	93	from	Effects	11:17	arg1	Lactoferrin					52:62	Bovine Lactoferrin	45:62	Bovine Lactoferrin	45:62	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.					
32477333	9	94	dep	0.5-fold	1610:1617	arg1	p					1620:1620	p < 0.0001	1620:1629	p < 0.0001	1620:1629	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	13	95	theme	human	2287:2291	arg1	MoDCs					2293:2297	human MoDCs	2287:2297	human MoDCs	2287:2297	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	0	96	from	Lactoferrin	52:62	arg1	Effects					11:17	Inhibitory Effects	0:17	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8	0:86	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.					
32477333	0	96	from	Lactoferrin	52:62	arg1	N-Glycans					30:38	Dietary N-Glycans	22:38	Dietary N-Glycans From Bovine Lactoferrin	22:62	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.					
32477333	12	97	theme	specific	2076:2083	arg1	N-glycans					2062:2070	the N-glycans	2058:2070	the N-glycans	2058:2070	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	12	97	theme	specific	2076:2083	arg1	inhibitors					2085:2094	specific inhibitors	2076:2094	specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs	2076:2146	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	8	98	theme	N-glycans	1260:1268	arg1	charge					1246:1251	charge	1246:1251	charge of the N-glycans	1246:1268	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	10	99	theme	native	1885:1890	arg1	N-glycans					1892:1900	the native N-glycans	1881:1900	the native N-glycans	1881:1900	Furthermore, the partially demannosylated and partially desialylated N-glycans showed stronger inhibition of IL-6 production compared with the native N-glycans.					
32477333	7	100	theme	Inhibitory	1100:1109	arg1	effects					1111:1117	Inhibitory effects	1100:1117	Inhibitory effects of the N-glycans	1100:1134	Inhibitory effects of the N-glycans were not influenced by the partially demannosylated or partially desialylated N-glycans.					
32477333	0	101	dep	Comparing	89:97	arg1	Effects					11:17	Inhibitory Effects	0:17	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8	0:86	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.					
32477333	12	102	theme	IL-6	2123:2126	arg1	production					2128:2137	IL-6 production	2123:2137	IL-6 production in MoDCs	2123:2146	Compared to CQN, the N-glycans are specific inhibitors of TLR-8 activation and of IL-6 production in MoDCs.					
32477333	8	103	with	interaction	1407:1417	arg1	receptor					1428:1435	the receptor	1424:1435	the receptor	1424:1435	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	5	104	theme	induced	871:877	arg1	activation					885:894	the ssRNA40 induced TLR-8 activation	859:894	the ssRNA40 induced TLR-8 activation	859:894	We found that in HEK293 cells, N-glycans strongly inhibited the ssRNA40 induced TLR-8 activation but to a lesser extent the R848 induced TLR-8 activation.					
32477333	4	105	link	monocyte-derived	757:772	arg1	MoDCs					791:795	MoDCs	791:795	MoDCs	791:795	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	4	105	link	monocyte-derived	757:772	arg1	cells					784:788	human monocyte-derived dendritic cells	751:788	human monocyte-derived dendritic cells (MoDCs)	751:796	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	2	106	theme	inflammatory	418:429	arg1	conditions					431:440	inflammatory conditions	418:440	inflammatory conditions	418:440	Dietary interventions are becoming more accepted as mean to manage onset, progression, and treatment of a broad spectrum of inflammatory conditions.					
32477333	2	107	dep	becoming	320:327	arg1	accepted					334:341	accepted	334:341	are becoming more accepted as mean to manage onset, progression, and treatment of a broad spectrum of inflammatory conditions	316:440	Dietary interventions are becoming more accepted as mean to manage onset, progression, and treatment of a broad spectrum of inflammatory conditions.					
32477333	0	108	theme	Dietary	22:28	arg1	N-Glycans					30:38	Dietary N-Glycans	22:38	Dietary N-Glycans From Bovine Lactoferrin	22:62	Inhibitory Effects of Dietary N-Glycans From Bovine Lactoferrin on Toll-Like Receptor 8; Comparing Efficacy With Chloroquine.					
32477333	1	109	theme	life	276:279	arg1	quality					265:271	quality	265:271	quality of life of patients	265:291	Toll-like receptor 8 (TLR-8) plays a role in the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients.					
32477333	7	110	gly	demannosylated	1173:1186	arg1	N-glycans					1214:1222	the partially demannosylated or partially desialylated N-glycans	1159:1222	the partially demannosylated or partially desialylated N-glycans	1159:1222	Inhibitory effects of the N-glycans were not influenced by the partially demannosylated or partially desialylated N-glycans.					
32477333	9	111	theme	<	1671:1671	arg1	p					1669:1669	p < 0.0001	1669:1678	p < 0.0001	1669:1678	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	8	112	theme	pH	1461:1462	arg1	changes					1464:1470	pH changes	1461:1470	pH changes in the endosome	1461:1486	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	5	113	theme	lesser	905:910	arg1	extent					912:917	a lesser extent	903:917	a lesser extent	903:917	We found that in HEK293 cells, N-glycans strongly inhibited the ssRNA40 induced TLR-8 activation but to a lesser extent the R848 induced TLR-8 activation.					
32477333	1	114	theme	patients	284:291	arg1	life					276:279	life	276:279	life of patients	276:291	Toll-like receptor 8 (TLR-8) plays a role in the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients.					
32477333	13	115	theme	TLR-8	2235:2239	arg1	activation					2256:2265	TLR-8 induced immune activation	2235:2265	TLR-8 induced immune activation in HEK293 cells and human MoDCs	2235:2297	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	8	116	theme	TLR-8	1315:1319	arg1	capacity					1303:1310	the inhibition capacity	1288:1310	the inhibition capacity of TLR-8	1288:1319	As the difference in charge of the N-glycans did not influence the inhibition capacity of TLR-8, it is possible that the inhibition mediated by the N-glycans is a result of a direct interaction with the receptor rather than a result of pH changes in the endosome.					
32477333	14	117	theme	food-based	2387:2396	arg1	strategy					2398:2405	a food-based strategy	2385:2405	a food-based strategy to manage autoimmune, infections or other inflammatory disorders	2385:2470	The inhibitory capacity of N-glycans isolated from bLF onTLR-8 activation may become a food-based strategy to manage autoimmune, infections or other inflammatory disorders.					
32477333	13	118	theme	immune	2249:2254	arg1	activation					2256:2265	TLR-8 induced immune activation	2235:2265	TLR-8 induced immune activation in HEK293 cells and human MoDCs	2235:2297	Our findings demonstrate that isolated N-glycans from bLF have attenuating effects on TLR-8 induced immune activation in HEK293 cells and human MoDCs.					
32477333	7	119	theme	desialylated	1201:1212	arg1	N-glycans					1214:1222	the partially demannosylated or partially desialylated N-glycans	1159:1222	the partially demannosylated or partially desialylated N-glycans	1159:1222	Inhibitory effects of the N-glycans were not influenced by the partially demannosylated or partially desialylated N-glycans.					
32477333	4	120	theme	dendritic	774:782	arg1	MoDCs					791:795	MoDCs	791:795	MoDCs	791:795	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	4	120	theme	dendritic	774:782	arg1	cells					784:788	human monocyte-derived dendritic cells	751:788	human monocyte-derived dendritic cells (MoDCs)	751:796	We investigated the effects of N-glycans in their native form, as well as in its partially demannosylated and partially desialylated form, on HEK293 cells expressing TLR-8, and in human monocyte-derived dendritic cells (MoDCs).					
32477333	9	121	theme	TLR-8	1507:1511	arg1	inhibition					1493:1502	The inhibition	1489:1502	The inhibition of TLR-8 in MoDCs	1489:1520	The inhibition of TLR-8 in MoDCs resulted in a significant decrease of IL-6 when cells were treated with the unmodified (0.5-fold, p < 0.0001), partially demannosylated (0.3-fold, p < 0.0001) and partially desialylated (0.4-fold, p < 0.0001) N-glycans.					
32477333	1	122	theme	gastrointestinal	227:242	arg1	symptoms					244:251	associated gastrointestinal symptoms	216:251	associated gastrointestinal symptoms	216:251	Toll-like receptor 8 (TLR-8) plays a role in the pathogenesis of autoimmune disorders and associated gastrointestinal symptoms that reduce quality of life of patients.					
34663219	10	0	from	perspective	1959:1969	arg1	environment					1928:1938	environment	1928:1938	environment	1928:1938	Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.					
34663219	10	0	from	perspective	1959:1969	arg1	genes					1944:1948	genes	1944:1948	genes	1944:1948	Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.					
34663219	10	1	theme	glycan-related	2010:2023	arg1	genes					2025:2029	glycan-related genes	2010:2029	glycan-related genes that exist specifically in the environment	2010:2072	Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.					
34663219	9	2	theme	genes	1730:1734	arg1	composition					1694:1704	the composition	1690:1704	the composition of their glycan-related genes to adapt the processes involved in acquiring energy from glycans in their environments	1690:1821	CONCLUSION These findings suggest that environmental microorganisms could change the composition of their glycan-related genes to adapt the processes involved in acquiring energy from glycans in their environments.					
34663219	8	3	theme	polysaccharide	1575:1588	arg1	enzymes					1600:1606	polysaccharide degrading enzymes	1575:1606	polysaccharide degrading enzymes	1575:1606	The general enzyme families were mostly from genes involved in monosaccharide metabolism, and most of the specific enzymes were polysaccharide degrading enzymes.					
34663219	1	4	theme	energy	172:177	arg1	acquisition					179:189	energy acquisition	172:189	energy acquisition	172:189	BACKGROUND Glycan-related genes play a fundamental role in various processes for energy acquisition and homeostasis maintenance while adapting to the environment in which the organism exists; however, their role in the microbiome in the environment is unclear.					
34663219	10	5	theme	carbohydrates	1974:1986	arg1	perspective					1959:1969	the perspective	1955:1969	the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment	1955:2072	Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.					
34663219	6	6	theme	%	1209:1209	arg1	coverage					1190:1197	a coverage	1188:1197	a coverage of over 80% in all the environments	1188:1233	On the other hand, the relative abundances of both glycoside hydrolases and glycosyltransferases surprisingly had a coverage of over 80% in all the environments.					
34663219	7	7	theme	enzymes	1403:1409	arg1	groups					1313:1318	two groups	1309:1318	two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments	1309:1444	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	7	7	theme	enzymes	1403:1409	arg1	enzymes					1403:1409	specific enzymes	1394:1409	(2) specific enzymes found only in certain environments	1390:1444	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	7	7	theme	enzymes	1403:1409	arg1	families					1342:1349	general enzyme families	1327:1349	(1) general enzyme families identified in various environments	1323:1384	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	8	8	theme	specific	1553:1560	arg1	enzymes					1562:1568	the specific enzymes	1549:1568	the specific enzymes	1549:1568	The general enzyme families were mostly from genes involved in monosaccharide metabolism, and most of the specific enzymes were polysaccharide degrading enzymes.					
34663219	10	9	theme	existence	1997:2005	arg1	perspective					1959:1969	the perspective	1955:1969	the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment	1955:2072	Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.					
34663219	5	10	theme	genes	929:933	arg1	high					939:942	high	939:942	high	939:942	Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments.					
34663219	5	10	theme	genes	929:933	arg1	percentage					900:909	the percentage	896:909	the percentage of glycan-related genes	896:933	Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments.					
34663219	2	11	theme	Sequence	360:367	arg1	alignment					369:377	METHODS Sequence alignment	352:377	METHODS Sequence alignment	352:377	METHODS Sequence alignment was performed between known glycan-related genes and complete genomes of microorganisms, and optimal parameters for identifying glycan-related genes were determined based on the alignments.					
34663219	9	12	dep	CONCLUSION	1609:1618	arg1	suggest					1635:1641	suggest	1635:1641	suggest that environmental microorganisms could change the composition of their glycan-related genes to adapt the processes involved in acquiring energy from glycans in their environments	1635:1821	CONCLUSION These findings suggest that environmental microorganisms could change the composition of their glycan-related genes to adapt the processes involved in acquiring energy from glycans in their environments.					
34663219	5	13	theme	glycan	1024:1029	arg1	metabolism					1031:1040	glycan metabolism	1024:1040	glycan metabolism better than other environments	1024:1071	Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments.					
34663219	6	14	theme	hydrolases	1135:1144	arg1	abundances					1106:1115	the relative abundances	1093:1115	the relative abundances of both glycoside hydrolases and glycosyltransferases	1093:1169	On the other hand, the relative abundances of both glycoside hydrolases and glycosyltransferases surprisingly had a coverage of over 80% in all the environments.					
34663219	3	15	theme	different	721:729	arg1	data					742:745	198 different metagenome data	717:745	198 different metagenome data	717:745	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	8	16	theme	general	1451:1457	arg1	families					1466:1473	The general enzyme families	1447:1473	The general enzyme families	1447:1473	The general enzyme families were mostly from genes involved in monosaccharide metabolism, and most of the specific enzymes were polysaccharide degrading enzymes.					
34663219	3	17	theme	constructed	579:589	arg1	scheme					591:596	the constructed scheme	575:596	the constructed scheme (> 90% of identity and > 25 aa of alignment length)	575:648	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	5	18	theme	better	1042:1047	arg1	metabolism					1031:1040	glycan metabolism	1024:1040	glycan metabolism better than other environments	1024:1071	Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments.					
34663219	3	19	from	genes	666:670	arg1	environments					683:694	various environments	675:694	various environments	675:694	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	9	20	theme	environmental	1648:1660	arg1	microorganisms					1662:1675	environmental microorganisms	1648:1675	environmental microorganisms	1648:1675	CONCLUSION These findings suggest that environmental microorganisms could change the composition of their glycan-related genes to adapt the processes involved in acquiring energy from glycans in their environments.					
34663219	4	21	theme	86.73	783:787	arg1	result					761:766	a result	759:766	a result	759:766	RESULTS As a result, we identified 86.73 million glycan-related genes from the metagenome data.					
34663219	4	21	theme	86.73	783:787	arg1	genes					812:816	86.73 million glycan-related genes	783:816	86.73 million glycan-related genes from the metagenome data	783:841	RESULTS As a result, we identified 86.73 million glycan-related genes from the metagenome data.					
34663219	3	22	dep	scheme	591:596	arg1	aa					626:627	identity and > 25 aa	608:627	identity and > 25 aa of alignment length	608:647	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	3	22	dep	scheme	591:596	arg1	%					603:603	> 90%	599:603	> 90% of identity and > 25 aa of alignment length	599:647	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	7	23	dep	families	1342:1349	arg1	1					1324:1324	1	1324:1324	1	1324:1324	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	2	24	theme	METHODS	352:358	arg1	alignment					369:377	METHODS Sequence alignment	352:377	METHODS Sequence alignment	352:377	METHODS Sequence alignment was performed between known glycan-related genes and complete genomes of microorganisms, and optimal parameters for identifying glycan-related genes were determined based on the alignments.					
34663219	10	25	theme	glyco-metagenomics	1839:1856	arg1	approach					1858:1865	Our functional glyco-metagenomics approach	1824:1865	Our functional glyco-metagenomics approach	1824:1865	Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.					
34663219	7	26	theme	various	1365:1371	arg1	environments					1373:1384	various environments	1365:1384	various environments	1365:1384	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	7	27	dep	enzymes	1403:1409	arg1	2					1391:1391	2	1391:1391	2	1391:1391	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	7	28	located	found	1411:1415	arg1	environments					1433:1444	certain environments	1425:1444	certain environments	1425:1444	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	7	28	located	found	1411:1415	arg2	enzymes					1403:1409	specific enzymes	1394:1409	(2) specific enzymes found only in certain environments	1390:1444	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	8	29	theme	degrading	1590:1598	arg1	enzymes					1600:1606	polysaccharide degrading enzymes	1575:1606	polysaccharide degrading enzymes	1575:1606	The general enzyme families were mostly from genes involved in monosaccharide metabolism, and most of the specific enzymes were polysaccharide degrading enzymes.					
34663219	9	30	from	glycans	1793:1799	arg1	energy					1781:1786	energy	1781:1786	energy from glycans	1781:1799	CONCLUSION These findings suggest that environmental microorganisms could change the composition of their glycan-related genes to adapt the processes involved in acquiring energy from glycans in their environments.					
34663219	1	31	theme	BACKGROUND	91:100	arg1	genes					117:121	BACKGROUND Glycan-related genes	91:121	BACKGROUND Glycan-related genes	91:121	BACKGROUND Glycan-related genes play a fundamental role in various processes for energy acquisition and homeostasis maintenance while adapting to the environment in which the organism exists; however, their role in the microbiome in the environment is unclear.					
34663219	1	32	theme	homeostasis	195:205	arg1	maintenance					207:217	homeostasis maintenance	195:217	homeostasis maintenance	195:217	BACKGROUND Glycan-related genes play a fundamental role in various processes for energy acquisition and homeostasis maintenance while adapting to the environment in which the organism exists; however, their role in the microbiome in the environment is unclear.					
34663219	2	33	theme	optimal	472:478	arg1	parameters					480:489	optimal parameters	472:489	optimal parameters for identifying glycan-related genes	472:526	METHODS Sequence alignment was performed between known glycan-related genes and complete genomes of microorganisms, and optimal parameters for identifying glycan-related genes were determined based on the alignments.					
34663219	0	34	from	role	45:48	arg1	environments					77:88	environments	77:88	environments	77:88	Functional glyco-metagenomics elucidates the role of glycan-related genes in environments.					
34663219	7	35	theme	enzyme	1335:1340	arg1	families					1342:1349	general enzyme families	1327:1349	(1) general enzyme families identified in various environments	1323:1384	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	1	36	theme	Glycan-related	102:115	arg1	genes					117:121	BACKGROUND Glycan-related genes	91:121	BACKGROUND Glycan-related genes	91:121	BACKGROUND Glycan-related genes play a fundamental role in various processes for energy acquisition and homeostasis maintenance while adapting to the environment in which the organism exists; however, their role in the microbiome in the environment is unclear.					
34663219	2	37	theme	known	401:405	arg1	genes					422:426	known glycan-related genes	401:426	known glycan-related genes	401:426	METHODS Sequence alignment was performed between known glycan-related genes and complete genomes of microorganisms, and optimal parameters for identifying glycan-related genes were determined based on the alignments.					
34663219	0	38	theme	Functional	0:9	arg1	glyco-metagenomics					11:28	Functional glyco-metagenomics	0:28	Functional glyco-metagenomics	0:28	Functional glyco-metagenomics elucidates the role of glycan-related genes in environments.					
34663219	1	39	from	role	298:301	arg1	microbiome					310:319	the microbiome	306:319	the microbiome	306:319	BACKGROUND Glycan-related genes play a fundamental role in various processes for energy acquisition and homeostasis maintenance while adapting to the environment in which the organism exists; however, their role in the microbiome in the environment is unclear.					
34663219	1	39	from	role	298:301	arg1	environment					328:338	the environment	324:338	the environment	324:338	BACKGROUND Glycan-related genes play a fundamental role in various processes for energy acquisition and homeostasis maintenance while adapting to the environment in which the organism exists; however, their role in the microbiome in the environment is unclear.					
34663219	5	40	from	high	939:942	arg1	environment					968:978	the human-associated environment	947:978	the human-associated environment	947:978	Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments.					
34663219	6	41	contain	had	1184:1186	arg2	coverage					1190:1197	a coverage	1188:1197	a coverage of over 80% in all the environments	1188:1233	On the other hand, the relative abundances of both glycoside hydrolases and glycosyltransferases surprisingly had a coverage of over 80% in all the environments.					
34663219	6	41	contain	had	1184:1186	arg1	abundances					1106:1115	the relative abundances	1093:1115	the relative abundances of both glycoside hydrolases and glycosyltransferases	1093:1169	On the other hand, the relative abundances of both glycoside hydrolases and glycosyltransferases surprisingly had a coverage of over 80% in all the environments.					
34663219	1	42	from	role	142:145	arg1	processes					158:166	various processes	150:166	various processes	150:166	BACKGROUND Glycan-related genes play a fundamental role in various processes for energy acquisition and homeostasis maintenance while adapting to the environment in which the organism exists; however, their role in the microbiome in the environment is unclear.					
34663219	4	43	dep	RESULTS	748:754	arg1	identified					772:781	identified	772:781	identified 86.73 million glycan-related genes from the metagenome data	772:841	RESULTS As a result, we identified 86.73 million glycan-related genes from the metagenome data.					
34663219	2	44	theme	microorganisms	452:465	arg1	genes					422:426	known glycan-related genes	401:426	known glycan-related genes	401:426	METHODS Sequence alignment was performed between known glycan-related genes and complete genomes of microorganisms, and optimal parameters for identifying glycan-related genes were determined based on the alignments.					
34663219	2	44	theme	microorganisms	452:465	arg1	genomes					441:447	complete genomes	432:447	complete genomes of microorganisms	432:465	METHODS Sequence alignment was performed between known glycan-related genes and complete genomes of microorganisms, and optimal parameters for identifying glycan-related genes were determined based on the alignments.					
34663219	6	45	theme	glycoside	1125:1133	arg1	hydrolases					1135:1144	glycoside hydrolases	1125:1144	glycoside hydrolases	1125:1144	On the other hand, the relative abundances of both glycoside hydrolases and glycosyltransferases surprisingly had a coverage of over 80% in all the environments.					
34663219	2	46	theme	glycan-related	507:520	arg1	genes					522:526	glycan-related genes	507:526	glycan-related genes	507:526	METHODS Sequence alignment was performed between known glycan-related genes and complete genomes of microorganisms, and optimal parameters for identifying glycan-related genes were determined based on the alignments.					
34663219	5	47	theme	other	1054:1058	arg1	environments					1060:1071	other environments	1054:1071	other environments	1054:1071	Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments.					
34663219	3	48	theme	various	675:681	arg1	environments					683:694	various environments	675:694	various environments	675:694	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	3	49	theme	aa	626:627	arg1	aa					626:627	identity and > 25 aa	608:627	identity and > 25 aa of alignment length	608:647	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	3	49	theme	aa	626:627	arg1	%					603:603	> 90%	599:603	> 90% of identity and > 25 aa of alignment length	599:647	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	6	50	theme	relative	1097:1104	arg1	abundances					1106:1115	the relative abundances	1093:1115	the relative abundances of both glycoside hydrolases and glycosyltransferases	1093:1169	On the other hand, the relative abundances of both glycoside hydrolases and glycosyltransferases surprisingly had a coverage of over 80% in all the environments.					
34663219	2	51	theme	complete	432:439	arg1	genomes					441:447	complete genomes	432:447	complete genomes of microorganisms	432:465	METHODS Sequence alignment was performed between known glycan-related genes and complete genomes of microorganisms, and optimal parameters for identifying glycan-related genes were determined based on the alignments.					
34663219	10	52	dep	environment	1928:1938	arg1	the					1924:1926	the	1924:1926	the	1924:1926	Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.					
34663219	3	53	theme	glycan-related	651:664	arg1	genes					666:670	glycan-related genes	651:670	glycan-related genes in various environments	651:694	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	7	54	theme	certain	1425:1431	arg1	environments					1433:1444	certain environments	1425:1444	certain environments	1425:1444	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	6	55	theme	other	1081:1085	arg1	hand					1087:1090	the other hand	1077:1090	the other hand	1077:1090	On the other hand, the relative abundances of both glycoside hydrolases and glycosyltransferases surprisingly had a coverage of over 80% in all the environments.					
34663219	0	56	theme	genes	68:72	arg1	role					45:48	the role	41:48	the role of glycan-related genes in environments	41:88	Functional glyco-metagenomics elucidates the role of glycan-related genes in environments.					
34663219	7	57	theme	glycoside	1242:1250	arg1	hydrolases					1252:1261	These glycoside hydrolases	1236:1261	These glycoside hydrolases	1236:1261	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	3	58	theme	identity	608:615	arg1	aa					626:627	identity and > 25 aa	608:627	identity and > 25 aa of alignment length	608:647	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	1	59	theme	fundamental	130:140	arg1	role					142:145	a fundamental role	128:145	a fundamental role in various processes for energy acquisition and homeostasis maintenance	128:217	BACKGROUND Glycan-related genes play a fundamental role in various processes for energy acquisition and homeostasis maintenance while adapting to the environment in which the organism exists; however, their role in the microbiome in the environment is unclear.					
34663219	0	60	theme	glycan-related	53:66	arg1	genes					68:72	glycan-related genes	53:72	glycan-related genes	53:72	Functional glyco-metagenomics elucidates the role of glycan-related genes in environments.					
34663219	10	61	theme	genes	2025:2029	arg1	existence					1997:2005	the existence	1993:2005	the existence of glycan-related genes that exist specifically in the environment	1993:2072	Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.					
34663219	10	61	theme	genes	2025:2029	arg1	carbohydrates					1974:1986	carbohydrates	1974:1986	carbohydrates	1974:1986	Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.					
34663219	6	62	from	coverage	1190:1197	arg1	environments					1222:1233	all the environments	1214:1233	all the environments	1214:1233	On the other hand, the relative abundances of both glycoside hydrolases and glycosyltransferases surprisingly had a coverage of over 80% in all the environments.					
34663219	7	63	theme	general	1327:1333	arg1	families					1342:1349	general enzyme families	1327:1349	(1) general enzyme families identified in various environments	1323:1384	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	4	64	theme	metagenome	827:836	arg1	data					838:841	the metagenome data	823:841	the metagenome data	823:841	RESULTS As a result, we identified 86.73 million glycan-related genes from the metagenome data.					
34663219	8	65	theme	monosaccharide	1510:1523	arg1	metabolism					1525:1534	monosaccharide metabolism	1510:1534	monosaccharide metabolism	1510:1534	The general enzyme families were mostly from genes involved in monosaccharide metabolism, and most of the specific enzymes were polysaccharide degrading enzymes.					
34663219	7	66	theme	specific	1394:1401	arg1	enzymes					1403:1409	specific enzymes	1394:1409	(2) specific enzymes found only in certain environments	1390:1444	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	9	67	theme	glycan-related	1715:1728	arg1	genes					1730:1734	their glycan-related genes	1709:1734	their glycan-related genes	1709:1734	CONCLUSION These findings suggest that environmental microorganisms could change the composition of their glycan-related genes to adapt the processes involved in acquiring energy from glycans in their environments.					
34663219	4	68	theme	glycan-related	797:810	arg1	result					761:766	a result	759:766	a result	759:766	RESULTS As a result, we identified 86.73 million glycan-related genes from the metagenome data.					
34663219	4	68	theme	glycan-related	797:810	arg1	genes					812:816	86.73 million glycan-related genes	783:816	86.73 million glycan-related genes from the metagenome data	783:841	RESULTS As a result, we identified 86.73 million glycan-related genes from the metagenome data.					
34663219	4	69	theme	million	789:795	arg1	result					761:766	a result	759:766	a result	759:766	RESULTS As a result, we identified 86.73 million glycan-related genes from the metagenome data.					
34663219	4	69	theme	million	789:795	arg1	genes					812:816	86.73 million glycan-related genes	783:816	86.73 million glycan-related genes from the metagenome data	783:841	RESULTS As a result, we identified 86.73 million glycan-related genes from the metagenome data.					
34663219	4	70	from	data	838:841	arg1	result					761:766	a result	759:766	a result	759:766	RESULTS As a result, we identified 86.73 million glycan-related genes from the metagenome data.					
34663219	4	70	from	data	838:841	arg1	genes					812:816	86.73 million glycan-related genes	783:816	86.73 million glycan-related genes from the metagenome data	783:841	RESULTS As a result, we identified 86.73 million glycan-related genes from the metagenome data.					
34663219	1	71	theme	various	150:156	arg1	processes					158:166	various processes	150:166	various processes	150:166	BACKGROUND Glycan-related genes play a fundamental role in various processes for energy acquisition and homeostasis maintenance while adapting to the environment in which the organism exists; however, their role in the microbiome in the environment is unclear.					
34663219	5	72	from	environment	968:978	arg1	high					939:942	high	939:942	high	939:942	Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments.					
34663219	5	72	from	environment	968:978	arg1	percentage					900:909	the percentage	896:909	the percentage of glycan-related genes	896:933	Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments.					
34663219	5	73	theme	human-associated	951:966	arg1	environment					968:978	the human-associated environment	947:978	the human-associated environment	947:978	Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments.					
34663219	8	74	theme	enzyme	1459:1464	arg1	families					1466:1473	The general enzyme families	1447:1473	The general enzyme families	1447:1473	The general enzyme families were mostly from genes involved in monosaccharide metabolism, and most of the specific enzymes were polysaccharide degrading enzymes.					
34663219	7	75	theme	families	1342:1349	arg1	groups					1313:1318	two groups	1309:1318	two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments	1309:1444	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	7	75	theme	families	1342:1349	arg1	enzymes					1403:1409	specific enzymes	1394:1409	(2) specific enzymes found only in certain environments	1390:1444	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	7	75	theme	families	1342:1349	arg1	families					1342:1349	general enzyme families	1327:1349	(1) general enzyme families identified in various environments	1323:1384	These glycoside hydrolases and glycosyltransferases were classified into two groups of (1) general enzyme families identified in various environments and (2) specific enzymes found only in certain environments.					
34663219	3	76	theme	alignment	632:640	arg1	length					642:647	alignment length	632:647	alignment length	632:647	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	6	77	theme	glycosyltransferases	1150:1169	arg1	abundances					1106:1115	the relative abundances	1093:1115	the relative abundances of both glycoside hydrolases and glycosyltransferases	1093:1169	On the other hand, the relative abundances of both glycoside hydrolases and glycosyltransferases surprisingly had a coverage of over 80% in all the environments.					
34663219	2	78	theme	glycan-related	407:420	arg1	genes					422:426	known glycan-related genes	401:426	known glycan-related genes	401:426	METHODS Sequence alignment was performed between known glycan-related genes and complete genomes of microorganisms, and optimal parameters for identifying glycan-related genes were determined based on the alignments.					
34663219	10	79	theme	functional	1828:1837	arg1	approach					1858:1865	Our functional glyco-metagenomics approach	1824:1865	Our functional glyco-metagenomics approach	1824:1865	Our functional glyco-metagenomics approach has made it possible to clarify the relationship between the environment and genes from the perspective of carbohydrates, and the existence of glycan-related genes that exist specifically in the environment.					
34663219	3	80	theme	metagenome	731:740	arg1	data					742:745	198 different metagenome data	717:745	198 different metagenome data	717:745	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	3	81	theme	length	642:647	arg1	aa					626:627	identity and > 25 aa	608:627	identity and > 25 aa of alignment length	608:647	Using the constructed scheme (> 90% of identity and > 25 aa of alignment length), glycan-related genes in various environments were identified from 198 different metagenome data.					
34663219	5	82	theme	glycan-related	914:927	arg1	genes					929:933	glycan-related genes	914:933	glycan-related genes	914:933	Among the 12 environments classified in this study, the percentage of glycan-related genes was high in the human-associated environment, suggesting that these environments utilize glycan metabolism better than other environments.					
33142627	5	0	theme	miscanthus-polyethylene	795:817	arg1	composites					819:828	the belowground miscanthus-polyethylene composites	779:828	the belowground miscanthus-polyethylene composites	779:828	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	3	1	from	different	429:437	arg1	belowground					457:467	miscanthus belowground	446:467	miscanthus belowground	446:467	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	3	1	from	different	429:437	arg1	biomass					485:491	aboveground biomass	473:491	aboveground biomass	473:491	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	5	2	theme	composites	819:828	arg1	properties					765:774	The mechanical properties	750:774	The mechanical properties of the belowground miscanthus-polyethylene composites	750:828	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	4	3	theme	high	549:552	arg1	level					554:558	a high level	547:558	a high level of xylose	547:568	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	2	4	theme	cell	311:314	arg1	contents					357:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	0	5	theme	polyethylene	90:101	arg1	composites					103:112	polyethylene composites	90:112	polyethylene composites	90:112	Polysaccharides and phenolics of miscanthus belowground cell walls and their influence on polyethylene composites.					
33142627	0	6	from	walls	61:65	arg1	composites					103:112	polyethylene composites	90:112	polyethylene composites	90:112	Polysaccharides and phenolics of miscanthus belowground cell walls and their influence on polyethylene composites.					
33142627	5	7	theme	most	1043:1046	arg1	sugars					1063:1068	most non-cellulosic sugars	1043:1068	most non-cellulosic sugars	1043:1068	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	1	8	from	species	157:163	arg1	materials					127:135	Belowground materials	115:135	Belowground materials from two miscanthus species	115:163	Belowground materials from two miscanthus species were ground into fragments for preparing polyethylene composites.					
33142627	2	9	theme	lignin	300:305	arg1	terms					274:278	terms	274:278	terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents	274:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	1	10	theme	miscanthus	146:155	arg1	species					157:163	two miscanthus species	142:163	two miscanthus species	142:163	Belowground materials from two miscanthus species were ground into fragments for preparing polyethylene composites.					
33142627	5	11	theme	non-cellulosic	1048:1061	arg1	sugars					1063:1068	most non-cellulosic sugars	1043:1068	most non-cellulosic sugars	1043:1068	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	4	12	theme	belowground	687:697	arg1	arabinoxylans					699:711	belowground arabinoxylans	687:711	belowground arabinoxylans	687:711	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	4	13	theme	samples	529:535	arg1	fraction					513:520	The non-cellulosic fraction	494:520	The non-cellulosic fraction of the samples	494:535	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	2	14	theme	polysaccharides	283:297	arg1	terms					274:278	terms	274:278	terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents	274:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	3	15	theme	aboveground	473:483	arg1	biomass					485:491	aboveground biomass	473:491	aboveground biomass	473:491	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	3	16	theme	lignins	408:414	arg1	structures					371:380	The structures	367:380	The structures of polysaccharides and of lignins	367:414	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	3	16	theme	lignins	408:414	arg1	different					429:437	different	429:437	different	429:437	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	2	17	from	lot	251:253	arg1	terms					274:278	terms	274:278	terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents	274:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	3	18	theme	miscanthus	446:455	arg1	belowground					457:467	miscanthus belowground	446:467	miscanthus belowground	446:467	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	5	19	theme	compositional	862:874	arg1	traits					876:881	their compositional traits	856:881	their compositional traits	856:881	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	4	20	theme	arabinose	580:588	arg1	ratio					600:604	the arabinose to xylose ratio	576:604	the arabinose to xylose ratio	576:604	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	2	21	theme	p-coumaric	328:337	arg1	contents					357:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	1	22	theme	polyethylene	206:217	arg1	composites					219:228	polyethylene composites	206:228	polyethylene composites	206:228	Belowground materials from two miscanthus species were ground into fragments for preparing polyethylene composites.					
33142627	5	23	theme	traits	876:881	arg1	several					845:851	several	845:851	several	845:851	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	2	24	theme	wall-linked	316:326	arg1	contents					357:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	2	25	from	similarities	258:269	arg1	terms					274:278	terms	274:278	terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents	274:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	4	26	theme	analogous	647:655	arg1	samples					662:668	analogous stem samples	647:668	analogous stem samples	647:668	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	4	27	theme	xylose	593:598	arg1	ratio					600:604	the arabinose to xylose ratio	576:604	the arabinose to xylose ratio	576:604	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	5	28	dep	lignin	977:982	arg1	contents					1004:1011	contents	1004:1011	contents	1004:1011	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	1	29	theme	Belowground	115:125	arg1	materials					127:135	Belowground materials	115:135	Belowground materials from two miscanthus species	115:163	Belowground materials from two miscanthus species were ground into fragments for preparing polyethylene composites.					
33142627	5	30	theme	similar	889:895	arg1	trends					897:902	similar trends	889:902	similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars	889:1068	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	5	31	theme	mechanical	754:763	arg1	properties					765:774	The mechanical properties	750:774	The mechanical properties of the belowground miscanthus-polyethylene composites	750:828	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	0	32	from	influence	77:85	arg1	composites					103:112	polyethylene composites	90:112	polyethylene composites	90:112	Polysaccharides and phenolics of miscanthus belowground cell walls and their influence on polyethylene composites.					
33142627	2	33	from	terms	274:278	arg1	lot					251:253	a lot	249:253	a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents	249:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	2	33	from	terms	274:278	arg1	similarities					258:269	similarities	258:269	similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents	258:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	0	34	theme	miscanthus	33:42	arg1	phenolics					20:28	phenolics	20:28	phenolics	20:28	Polysaccharides and phenolics of miscanthus belowground cell walls and their influence on polyethylene composites.					
33142627	0	34	theme	miscanthus	33:42	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides	0:14	Polysaccharides and phenolics of miscanthus belowground cell walls and their influence on polyethylene composites.					
33142627	2	35	link	wall-linked	316:326	arg1	contents					357:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	5	36	theme	stem-polyethylene	917:933	arg1	composites					935:944	plant stem-polyethylene composites	911:944	plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars	911:1068	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	4	37	theme	non-cellulosic	498:511	arg1	fraction					513:520	The non-cellulosic fraction	494:520	The non-cellulosic fraction of the samples	494:535	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	3	38	dep	belowground	457:467	arg1	the					442:444	the	442:444	the	442:444	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	3	39	from	biomass	485:491	arg1	different					429:437	different	429:437	different	429:437	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	3	39	from	biomass	485:491	arg1	structures					371:380	The structures	367:380	The structures of polysaccharides and of lignins	367:414	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	4	40	theme	stem	657:660	arg1	samples					662:668	analogous stem samples	647:668	analogous stem samples	647:668	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	4	41	theme	xylose	563:568	arg1	level					554:558	a high level	547:558	a high level of xylose	547:568	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	4	42	dep	ratio	600:604	arg1	to					590:591	to	590:591	to	590:591	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	4	43	theme	stem	739:742	arg1	ones					744:747	stem ones	739:747	stem ones	739:747	The non-cellulosic fraction of the samples comprises a high level of xylose, with the arabinose to xylose ratio about twice as high as that observed for analogous stem samples, suggesting that belowground arabinoxylans are more substituted than stem ones.					
33142627	0	44	theme	cell	56:59	arg1	walls					61:65	cell walls	56:65	cell walls	56:65	Polysaccharides and phenolics of miscanthus belowground cell walls and their influence on polyethylene composites.					
33142627	5	45	with	composites	935:944	arg1	correlations					960:971	positive correlations	951:971	positive correlations for lignin and p-coumaric acid contents	951:1011	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	5	45	with	composites	935:944	arg1	correlations					1026:1037	negative correlations	1017:1037	negative correlations for most non-cellulosic sugars	1017:1068	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	2	46	theme	contents	357:364	arg1	terms					274:278	terms	274:278	terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents	274:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	2	47	theme	acids	351:355	arg1	contents					357:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	5	48	theme	negative	1017:1024	arg1	correlations					1026:1037	negative correlations	1017:1037	negative correlations for most non-cellulosic sugars	1017:1068	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	2	49	theme	ferulic	343:349	arg1	contents					357:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	cell wall-linked p-coumaric and ferulic acids contents	311:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	5	50	theme	p-coumaric	988:997	arg1	acid					999:1002	p-coumaric acid	988:1002	p-coumaric acid	988:1002	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	3	51	from	belowground	457:467	arg1	different					429:437	different	429:437	different	429:437	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	3	51	from	belowground	457:467	arg1	structures					371:380	The structures	367:380	The structures of polysaccharides and of lignins	367:414	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	5	52	theme	plant	911:915	arg1	composites					935:944	plant stem-polyethylene composites	911:944	plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars	911:1068	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	5	53	theme	belowground	783:793	arg1	composites					819:828	the belowground miscanthus-polyethylene composites	779:828	the belowground miscanthus-polyethylene composites	779:828	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	2	54	theme	similarities	258:269	arg1	lot					251:253	a lot	249:253	a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents	249:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	2	54	theme	similarities	258:269	arg1	similarities					258:269	similarities	258:269	similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents	258:364	Both species show a lot of similarities in terms of polysaccharides, lignin and cell wall-linked p-coumaric and ferulic acids contents.					
33142627	5	55	theme	positive	951:958	arg1	correlations					960:971	positive correlations	951:971	positive correlations for lignin and p-coumaric acid contents	951:1011	The mechanical properties of the belowground miscanthus-polyethylene composites correlate with several of their compositional traits, with similar trends as for plant stem-polyethylene composites with positive correlations for lignin and p-coumaric acid contents and negative correlations for most non-cellulosic sugars.					
33142627	3	56	theme	polysaccharides	385:399	arg1	structures					371:380	The structures	367:380	The structures of polysaccharides and of lignins	367:414	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
33142627	3	56	theme	polysaccharides	385:399	arg1	different					429:437	different	429:437	different	429:437	The structures of polysaccharides and of lignins are markedly different in the miscanthus belowground and aboveground biomass.					
32297503	7	0	contain	possesses	1250:1258	arg1	method					1243:1248	The method	1239:1248	The method	1239:1248	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	7	0	contain	possesses	1250:1258	arg2	expansibility					1294:1306	potential expansibility	1284:1306	potential expansibility	1284:1306	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	7	0	contain	possesses	1250:1258	arg2	applicability					1266:1278	broad applicability	1260:1278	broad applicability	1260:1278	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	4	1	theme	gold	706:709	arg1	nanoparticles					711:723	gold nanoparticles	706:723	gold nanoparticles	706:723	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	1	2	theme	important	236:244	arg1	rafts					113:117	Lipid rafts	107:117	Lipid rafts	107:117	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	1	2	theme	important	236:244	arg1	platforms					257:265	important functional platforms	236:265	important functional platforms for regulation of lipid/protein interactions	236:310	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	5	3	theme	event	999:1003	arg1	translation					963:973	the translation	959:973	the translation of one raft-recognition event to multiple raft-confined labeling operations	959:1049	Taking advantage of the abundant glycoconjugates enriched in lipid rafts, this elaborate design achieves the translation of one raft-recognition event to multiple raft-confined labeling operations, thus, significantly increasing the labeling efficiency and imaging sensitivity.					
32297503	6	4	theme	long-term	1186:1194	arg1	tracking					1196:1203	in situ and long-term tracking	1174:1203	in situ and long-term tracking of raft components in live cells	1174:1236	The direct covalent labeling also enables in situ and long-term tracking of raft components in live cells.					
32297503	2	5	from	predicament	323:333	arg1	study					349:353	lipid raft study	338:353	lipid raft study	338:353	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	1	6	theme	functional	246:255	arg1	rafts					113:117	Lipid rafts	107:117	Lipid rafts	107:117	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	1	6	theme	functional	246:255	arg1	platforms					257:265	important functional platforms	236:265	important functional platforms for regulation of lipid/protein interactions	236:310	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	7	7	theme	broad	1260:1264	arg1	applicability					1266:1278	broad applicability	1260:1278	broad applicability	1260:1278	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	3	8	theme	enzymatic	496:504	arg1	strategy					523:530	a proximity enzymatic glyco-remodeling strategy	484:530	a proximity enzymatic glyco-remodeling strategy for direct and highly efficient lipid raft labeling and imaging on live cells	484:608	To solve this issue, we herein report a proximity enzymatic glyco-remodeling strategy for direct and highly efficient lipid raft labeling and imaging on live cells.					
32297503	3	9	theme	direct	536:541	arg1	labeling					575:582	direct and highly efficient lipid raft labeling	536:582	direct and highly efficient lipid raft labeling	536:582	To solve this issue, we herein report a proximity enzymatic glyco-remodeling strategy for direct and highly efficient lipid raft labeling and imaging on live cells.					
32297503	3	10	theme	efficient	554:562	arg1	labeling					575:582	direct and highly efficient lipid raft labeling	536:582	direct and highly efficient lipid raft labeling	536:582	To solve this issue, we herein report a proximity enzymatic glyco-remodeling strategy for direct and highly efficient lipid raft labeling and imaging on live cells.					
32297503	0	11	theme	Live	95:98	arg1	Cells					100:104	Live Cells	95:104	Live Cells	95:104	Proximity Enzymatic Glyco-Remodeling Enables Direct and Highly Efficient Lipid Raft Imaging on Live Cells.					
32297503	6	12	theme	components	1213:1222	arg1	tracking					1196:1203	in situ and long-term tracking	1174:1203	in situ and long-term tracking of raft components in live cells	1174:1236	The direct covalent labeling also enables in situ and long-term tracking of raft components in live cells.					
32297503	5	13	theme	multiple	1008:1015	arg1	operations					1040:1049	multiple raft-confined labeling operations	1008:1049	multiple raft-confined labeling operations	1008:1049	Taking advantage of the abundant glycoconjugates enriched in lipid rafts, this elaborate design achieves the translation of one raft-recognition event to multiple raft-confined labeling operations, thus, significantly increasing the labeling efficiency and imaging sensitivity.					
32297503	5	14	theme	glycoconjugates	887:901	arg1	advantage					861:869	advantage	861:869	advantage of the abundant glycoconjugates enriched in lipid rafts	861:925	Taking advantage of the abundant glycoconjugates enriched in lipid rafts, this elaborate design achieves the translation of one raft-recognition event to multiple raft-confined labeling operations, thus, significantly increasing the labeling efficiency and imaging sensitivity.					
32297503	6	15	theme	covalent	1143:1150	arg1	labeling					1152:1159	The direct covalent labeling	1132:1159	The direct covalent labeling	1132:1159	The direct covalent labeling also enables in situ and long-term tracking of raft components in live cells.					
32297503	5	16	theme	raft-confined	1017:1029	arg1	operations					1040:1049	multiple raft-confined labeling operations	1008:1049	multiple raft-confined labeling operations	1008:1049	Taking advantage of the abundant glycoconjugates enriched in lipid rafts, this elaborate design achieves the translation of one raft-recognition event to multiple raft-confined labeling operations, thus, significantly increasing the labeling efficiency and imaging sensitivity.					
32297503	2	17	theme	visualization	388:400	arg1	tools					402:406	direct and robust visualization tools	370:406	direct and robust visualization tools for in situ tracking raft components	370:443	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	6	18	theme	raft	1208:1211	arg1	components					1213:1222	raft components	1208:1222	raft components	1208:1222	The direct covalent labeling also enables in situ and long-term tracking of raft components in live cells.					
32297503	5	19	theme	labeling	1031:1038	arg1	operations					1040:1049	multiple raft-confined labeling operations	1008:1049	multiple raft-confined labeling operations	1008:1049	Taking advantage of the abundant glycoconjugates enriched in lipid rafts, this elaborate design achieves the translation of one raft-recognition event to multiple raft-confined labeling operations, thus, significantly increasing the labeling efficiency and imaging sensitivity.					
32297503	2	20	theme	robust	381:386	arg1	tools					402:406	direct and robust visualization tools	370:406	direct and robust visualization tools for in situ tracking raft components	370:443	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	0	21	theme	Enzymatic	10:18	arg1	Glyco-Remodeling					20:35	Proximity Enzymatic Glyco-Remodeling	0:35	Proximity Enzymatic Glyco-Remodeling	0:35	Proximity Enzymatic Glyco-Remodeling Enables Direct and Highly Efficient Lipid Raft Imaging on Live Cells.					
32297503	3	22	theme	glyco-remodeling	506:521	arg1	strategy					523:530	a proximity enzymatic glyco-remodeling strategy	484:530	a proximity enzymatic glyco-remodeling strategy for direct and highly efficient lipid raft labeling and imaging on live cells	484:608	To solve this issue, we herein report a proximity enzymatic glyco-remodeling strategy for direct and highly efficient lipid raft labeling and imaging on live cells.					
32297503	7	23	theme	potential	1284:1292	arg1	expansibility					1294:1306	potential expansibility	1284:1306	potential expansibility	1284:1306	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	3	24	theme	lipid	564:568	arg1	labeling					575:582	direct and highly efficient lipid raft labeling	536:582	direct and highly efficient lipid raft labeling	536:582	To solve this issue, we herein report a proximity enzymatic glyco-remodeling strategy for direct and highly efficient lipid raft labeling and imaging on live cells.					
32297503	4	25	theme	enzyme	696:701	arg1	cofunctionalization					619:637	cofunctionalization	619:637	cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles	619:723	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	0	26	theme	Proximity	0:8	arg1	Glyco-Remodeling					20:35	Proximity Enzymatic Glyco-Remodeling	0:35	Proximity Enzymatic Glyco-Remodeling	0:35	Proximity Enzymatic Glyco-Remodeling Enables Direct and Highly Efficient Lipid Raft Imaging on Live Cells.					
32297503	7	27	from	investigations	1343:1356	arg1	composition					1373:1383	the complex composition	1361:1383	the complex composition	1361:1383	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	7	27	from	investigations	1343:1356	arg1	organization					1386:1397	organization	1386:1397	organization	1386:1397	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	7	27	from	investigations	1343:1356	arg1	dynamics					1404:1411	dynamics	1404:1411	dynamics	1404:1411	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	2	28	theme	major	317:321	arg1	lack					362:365	the lack	358:365	the lack of direct and robust visualization tools for in situ tracking raft components	358:443	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	2	28	theme	major	317:321	arg1	predicament					323:333	The major predicament	313:333	The major predicament in lipid raft study	313:353	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	2	29	theme	direct	370:375	arg1	tools					402:406	direct and robust visualization tools	370:406	direct and robust visualization tools for in situ tracking raft components	370:443	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	2	30	theme	raft	429:432	arg1	components					434:443	in situ tracking raft components	412:443	in situ tracking raft components	412:443	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	6	31	theme	direct	1136:1141	arg1	labeling					1152:1159	The direct covalent labeling	1132:1159	The direct covalent labeling	1132:1159	The direct covalent labeling also enables in situ and long-term tracking of raft components in live cells.					
32297503	5	32	theme	labeling	1087:1094	arg1	efficiency					1096:1105	the labeling efficiency	1083:1105	the labeling efficiency	1083:1105	Taking advantage of the abundant glycoconjugates enriched in lipid rafts, this elaborate design achieves the translation of one raft-recognition event to multiple raft-confined labeling operations, thus, significantly increasing the labeling efficiency and imaging sensitivity.					
32297503	5	33	theme	raft-recognition	982:997	arg1	event					999:1003	one raft-recognition event	978:1003	one raft-recognition event to multiple raft-confined labeling operations	978:1049	Taking advantage of the abundant glycoconjugates enriched in lipid rafts, this elaborate design achieves the translation of one raft-recognition event to multiple raft-confined labeling operations, thus, significantly increasing the labeling efficiency and imaging sensitivity.					
32297503	2	34	theme	tracking	420:427	arg1	components					434:443	in situ tracking raft components	412:443	in situ tracking raft components	412:443	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	1	35	theme	lipid/protein	285:297	arg1	interactions					299:310	lipid/protein interactions	285:310	lipid/protein interactions	285:310	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	1	36	theme	Lipid	107:111	arg1	platforms					257:265	important functional platforms	236:265	important functional platforms for regulation of lipid/protein interactions	236:310	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	1	36	theme	Lipid	107:111	arg1	rafts					113:117	Lipid rafts	107:117	Lipid rafts	107:117	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	1	36	theme	Lipid	107:111	arg1	domains					149:155	highly ordered cell membrane domains	120:155	highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors	120:224	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	2	37	theme	in	412:413	arg1	components					434:443	in situ tracking raft components	412:443	in situ tracking raft components	412:443	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	7	38	theme	complex	1365:1371	arg1	composition					1373:1383	the complex composition	1361:1383	the complex composition	1361:1383	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	1	39	theme	interactions	299:310	arg1	regulation					271:280	regulation	271:280	regulation of lipid/protein interactions	271:310	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	4	40	theme	recognition	656:666	arg1	motif					668:672	raft-specific recognition motif	642:672	raft-specific recognition motif	642:672	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	4	41	theme	raft-specific	642:654	arg1	motif					668:672	raft-specific recognition motif	642:672	raft-specific recognition motif	642:672	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	4	42	theme	glycan-remodeling	678:694	arg1	enzyme					696:701	glycan-remodeling enzyme	678:701	glycan-remodeling enzyme	678:701	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	3	43	theme	raft	570:573	arg1	labeling					575:582	direct and highly efficient lipid raft labeling	536:582	direct and highly efficient lipid raft labeling	536:582	To solve this issue, we herein report a proximity enzymatic glyco-remodeling strategy for direct and highly efficient lipid raft labeling and imaging on live cells.					
32297503	0	44	theme	Direct	45:50	arg1	Imaging					84:90	Direct and Highly Efficient Lipid Raft Imaging	45:90	Direct and Highly Efficient Lipid Raft Imaging	45:90	Proximity Enzymatic Glyco-Remodeling Enables Direct and Highly Efficient Lipid Raft Imaging on Live Cells.					
32297503	4	45	theme	neighboring	833:843	arg1	glycans					845:851	neighboring glycans	833:851	neighboring glycans	833:851	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	5	46	theme	abundant	878:885	arg1	glycoconjugates					887:901	the abundant glycoconjugates	874:901	the abundant glycoconjugates enriched in lipid rafts	874:925	Taking advantage of the abundant glycoconjugates enriched in lipid rafts, this elaborate design achieves the translation of one raft-recognition event to multiple raft-confined labeling operations, thus, significantly increasing the labeling efficiency and imaging sensitivity.					
32297503	1	47	theme	protein	208:214	arg1	receptors					216:224	protein receptors	208:224	protein receptors	208:224	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	0	48	theme	Efficient	63:71	arg1	Imaging					84:90	Direct and Highly Efficient Lipid Raft Imaging	45:90	Direct and Highly Efficient Lipid Raft Imaging	45:90	Proximity Enzymatic Glyco-Remodeling Enables Direct and Highly Efficient Lipid Raft Imaging on Live Cells.					
32297503	6	49	dep	in	1174:1175	arg1	situ					1177:1180	situ	1177:1180	situ	1177:1180	The direct covalent labeling also enables in situ and long-term tracking of raft components in live cells.					
32297503	6	50	theme	in	1174:1175	arg1	tracking					1196:1203	in situ and long-term tracking	1174:1203	in situ and long-term tracking of raft components in live cells	1174:1236	The direct covalent labeling also enables in situ and long-term tracking of raft components in live cells.					
32297503	4	51	theme	fabricated	730:739	arg1	nanoprobe					741:749	the fabricated nanoprobe	726:749	the fabricated nanoprobe	726:749	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	7	52	dep	possesses	1250:1258	arg1	facilitate					1328:1337	facilitate	1328:1337	will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts	1315:1426	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	2	53	dep	in	412:413	arg1	situ					415:418	situ	415:418	situ	415:418	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	6	54	from	tracking	1196:1203	arg1	cells					1232:1236	live cells	1227:1236	live cells	1227:1236	The direct covalent labeling also enables in situ and long-term tracking of raft components in live cells.					
32297503	2	55	theme	raft	344:347	arg1	study					349:353	lipid raft study	338:353	lipid raft study	338:353	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	7	56	theme	rafts	1422:1426	arg1	composition					1373:1383	the complex composition	1361:1383	the complex composition	1361:1383	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	7	56	theme	rafts	1422:1426	arg1	organization					1386:1397	organization	1386:1397	organization	1386:1397	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	7	56	theme	rafts	1422:1426	arg1	dynamics					1404:1411	dynamics	1404:1411	dynamics	1404:1411	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	4	57	theme	catalytic	809:817	arg1	remodeling					819:828	catalytic remodeling	809:828	catalytic remodeling	809:828	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	2	58	theme	lipid	338:342	arg1	study					349:353	lipid raft study	338:353	lipid raft study	338:353	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	5	59	theme	lipid	915:919	arg1	rafts					921:925	lipid rafts	915:925	lipid rafts	915:925	Taking advantage of the abundant glycoconjugates enriched in lipid rafts, this elaborate design achieves the translation of one raft-recognition event to multiple raft-confined labeling operations, thus, significantly increasing the labeling efficiency and imaging sensitivity.					
32297503	6	60	theme	live	1227:1230	arg1	cells					1232:1236	live cells	1227:1236	live cells	1227:1236	The direct covalent labeling also enables in situ and long-term tracking of raft components in live cells.					
32297503	4	61	theme	raft	785:788	arg1	domains					790:796	the raft domains	781:796	the raft domains	781:796	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	1	62	theme	ordered	127:133	arg1	rafts					113:117	Lipid rafts	107:117	Lipid rafts	107:117	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	1	62	theme	ordered	127:133	arg1	domains					149:155	highly ordered cell membrane domains	120:155	highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors	120:224	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	0	63	theme	Raft	79:82	arg1	Imaging					84:90	Direct and Highly Efficient Lipid Raft Imaging	45:90	Direct and Highly Efficient Lipid Raft Imaging	45:90	Proximity Enzymatic Glyco-Remodeling Enables Direct and Highly Efficient Lipid Raft Imaging on Live Cells.					
32297503	2	64	theme	tools	402:406	arg1	lack					362:365	the lack	358:365	the lack of direct and robust visualization tools for in situ tracking raft components	358:443	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	2	64	theme	tools	402:406	arg1	predicament					323:333	The major predicament	313:333	The major predicament in lipid raft study	313:353	The major predicament in lipid raft study is the lack of direct and robust visualization tools for in situ tracking raft components.					
32297503	3	65	theme	live	599:602	arg1	cells					604:608	live cells	599:608	live cells	599:608	To solve this issue, we herein report a proximity enzymatic glyco-remodeling strategy for direct and highly efficient lipid raft labeling and imaging on live cells.					
32297503	1	66	theme	cell	135:138	arg1	rafts					113:117	Lipid rafts	107:117	Lipid rafts	107:117	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	1	66	theme	cell	135:138	arg1	domains					149:155	highly ordered cell membrane domains	120:155	highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors	120:224	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	4	67	theme	motif	668:672	arg1	cofunctionalization					619:637	cofunctionalization	619:637	cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles	619:723	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	0	68	theme	Lipid	73:77	arg1	Imaging					84:90	Direct and Highly Efficient Lipid Raft Imaging	45:90	Direct and Highly Efficient Lipid Raft Imaging	45:90	Proximity Enzymatic Glyco-Remodeling Enables Direct and Highly Efficient Lipid Raft Imaging on Live Cells.					
32297503	4	69	from	cofunctionalization	619:637	arg1	nanoparticles					711:723	gold nanoparticles	706:723	gold nanoparticles	706:723	Through cofunctionalization of raft-specific recognition motif and glycan-remodeling enzyme on gold nanoparticles, the fabricated nanoprobe can be specifically guided to the raft domains to perform catalytic remodeling on neighboring glycans.					
32297503	7	70	theme	lipid	1416:1420	arg1	rafts					1422:1426	lipid rafts	1416:1426	lipid rafts	1416:1426	The method possesses broad applicability and potential expansibility, thus, will greatly facilitate the investigations on the complex composition, organization, and dynamics of lipid rafts.					
32297503	1	71	theme	membrane	140:147	arg1	rafts					113:117	Lipid rafts	107:117	Lipid rafts	107:117	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	1	71	theme	membrane	140:147	arg1	domains					149:155	highly ordered cell membrane domains	120:155	highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors	120:224	Lipid rafts, highly ordered cell membrane domains mainly composed of cholesterol, sphingolipids, and protein receptors, serve as important functional platforms for regulation of lipid/protein interactions.					
32297503	3	72	from	strategy	523:530	arg1	cells					604:608	live cells	599:608	live cells	599:608	To solve this issue, we herein report a proximity enzymatic glyco-remodeling strategy for direct and highly efficient lipid raft labeling and imaging on live cells.					
33124956	7	0	theme	cytokine-accessible-signaling	1092:1120	arg1	assemblies					1123:1132	'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies	1049:1132	'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP	1049:1150	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	7	1	dep	form	1031:1034	arg1	types					1040:1044	two types	1036:1044	two types	1036:1044	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	10	2	theme	critical	1648:1655	arg1	role					1657:1660	critical role	1648:1660	critical role	1648:1660	Taken together, it was shown that full glycosylation regulates formation of the IL-1RI functional assembly and play critical role in cytokine biding and triggering the IL-1RI involved downstream pathways in the cell.Communicated by Ramaswamy H. Sarma.					
33124956	6	3	theme	glycosylation	891:903	arg1	role					878:881	the role	874:881	the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly	874:968	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	10	4	theme	cytokine	1665:1672	arg1	biding					1674:1679	cytokine biding	1665:1679	cytokine biding	1665:1679	Taken together, it was shown that full glycosylation regulates formation of the IL-1RI functional assembly and play critical role in cytokine biding and triggering the IL-1RI involved downstream pathways in the cell.Communicated by Ramaswamy H. Sarma.					
33124956	4	5	theme	dynamical	678:686	arg1	pattern					688:694	its dynamical pattern	674:694	its dynamical pattern	674:694	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
33124956	0	6	theme	description	98:108	arg1	completion					72:81	completion	72:81	completion of the current description for immune response	72:128	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.					
33124956	6	7	theme	full	886:889	arg1	glycosylation					891:903	full glycosylation	886:903	full glycosylation of IL-1RI and IL-1RAcP	886:926	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	10	8	theme	involved	1707:1714	arg1	pathways					1727:1734	the IL-1RI involved downstream pathways	1696:1734	the IL-1RI involved downstream pathways in the cell.Communicated	1696:1759	Taken together, it was shown that full glycosylation regulates formation of the IL-1RI functional assembly and play critical role in cytokine biding and triggering the IL-1RI involved downstream pathways in the cell.Communicated by Ramaswamy H. Sarma.					
33124956	7	9	with	assemblies	1123:1132	arg1	IL-1RacP					1143:1150	the IL-1RacP	1139:1150	the IL-1RacP	1139:1150	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	6	10	gly	glycosylation	891:903	arg1	IL-1RI					908:913	IL-1RI	908:913	IL-1RI	908:913	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	6	10	gly	glycosylation	891:903	arg1	IL-1RAcP					919:926	IL-1RAcP	919:926	IL-1RAcP	919:926	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	6	10	gly	glycosylation	891:903	arg1	assembly					961:968	the functional assembly	946:968	the functional assembly	946:968	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	0	11	theme	current	90:96	arg1	description					98:108	the current description	86:108	the current description for immune response	86:128	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.					
33124956	2	12	theme	action	292:297	arg1	mechanism					279:287	its detailed mechanism	266:287	its detailed mechanism of action	266:297	Understanding its detailed mechanism of action is crucial for treating immune disorders.					
33124956	9	13	theme	extended	1397:1404	arg1	complex					1407:1413	The 'extended' complex	1392:1413	The 'extended' complex	1392:1413	The 'extended' complex is maintained by formation of several persistent hydrogen bonds between the IL-1RI-IL-1RAcP inter-connected glycans.					
33124956	0	14	theme	immune	114:119	arg1	response					121:128	immune response	114:128	immune response	114:128	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.					
33124956	10	15	theme	IL-1RI	1700:1705	arg1	pathways					1727:1734	the IL-1RI involved downstream pathways	1696:1734	the IL-1RI involved downstream pathways in the cell.Communicated	1696:1759	Taken together, it was shown that full glycosylation regulates formation of the IL-1RI functional assembly and play critical role in cytokine biding and triggering the IL-1RI involved downstream pathways in the cell.Communicated by Ramaswamy H. Sarma.					
33124956	7	16	theme	cytokine-inaccessible-non-signaling	1050:1084	arg1	assemblies					1123:1132	'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies	1049:1132	'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP	1049:1150	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	4	17	theme	compact	625:631	arg1	states					664:669	'compact' and 'extended' conformational states	624:669	'compact' and 'extended' conformational states	624:669	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
33124956	8	18	theme	compact	1333:1339	arg1	complex					1342:1348	the 'compact' complex	1328:1348	the 'compact' complex	1328:1348	Suggesting that the cytokine binding to IL-1RI is not required for the formation of IL-1RI-IL-1RAcP complex and the 'compact' complex could act as a down-regulatory mechanism.					
33124956	8	18	theme	compact	1333:1339	arg1	mechanism					1381:1389	a down-regulatory mechanism	1363:1389	a down-regulatory mechanism	1363:1389	Suggesting that the cytokine binding to IL-1RI is not required for the formation of IL-1RI-IL-1RAcP complex and the 'compact' complex could act as a down-regulatory mechanism.					
33124956	8	19	theme	cytokine	1236:1243	arg1	binding					1245:1251	the cytokine binding	1232:1251	the cytokine binding to IL-1RI	1232:1261	Suggesting that the cytokine binding to IL-1RI is not required for the formation of IL-1RI-IL-1RAcP complex and the 'compact' complex could act as a down-regulatory mechanism.					
33124956	3	20	theme	cytokine	438:445	arg1	binding					418:424	binding	418:424	binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it	418:488	IL-1RI is activated upon formation of its functional assembly that occurs by binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it.					
33124956	6	21	from	role	878:881	arg1	arrangement					931:941	arrangement	931:941	arrangement of the functional assembly	931:968	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	10	22	theme	assembly	1630:1637	arg1	formation					1595:1603	formation	1595:1603	formation of the IL-1RI functional assembly	1595:1637	Taken together, it was shown that full glycosylation regulates formation of the IL-1RI functional assembly and play critical role in cytokine biding and triggering the IL-1RI involved downstream pathways in the cell.Communicated by Ramaswamy H. Sarma.					
33124956	4	23	theme	extended	639:646	arg1	states					664:669	'compact' and 'extended' conformational states	624:669	'compact' and 'extended' conformational states	624:669	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
33124956	0	24	theme	functional	16:25	arg1	assembly					27:34	Glycan-mediated functional assembly	0:34	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.	0:129	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.					
33124956	2	25	theme	immune	323:328	arg1	disorders					330:338	immune disorders	323:338	immune disorders	323:338	Understanding its detailed mechanism of action is crucial for treating immune disorders.					
33124956	7	26	dep	abiding	1180:1186	arg1	form					1031:1034	the 'compact' and 'extended' IL-1RI form two types	995:1044	form	1031:1034	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	4	27	theme	states	664:669	arg1	types					615:619	two types	611:619	two types of 'compact' and 'extended' conformational states	611:669	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
33124956	0	28	theme	Glycan-mediated	0:14	arg1	assembly					27:34	Glycan-mediated functional assembly	0:34	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.	0:129	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.					
33124956	6	29	theme	assembly	961:968	arg1	arrangement					931:941	arrangement	931:941	arrangement of the functional assembly	931:968	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	3	30	theme	IL-1	433:436	arg1	cytokine					438:445	the IL-1 cytokine	429:445	the IL-1 cytokine	429:445	IL-1RI is activated upon formation of its functional assembly that occurs by binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it.					
33124956	4	31	theme	molecular	547:555	arg1	dynamics					557:564	molecular dynamics	547:564	molecular dynamics	547:564	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
33124956	10	32	from	pathways	1727:1734	arg1	cell.Communicated					1743:1759	the cell.Communicated	1739:1759	the cell.Communicated	1739:1759	Taken together, it was shown that full glycosylation regulates formation of the IL-1RI functional assembly and play critical role in cytokine biding and triggering the IL-1RI involved downstream pathways in the cell.Communicated by Ramaswamy H. Sarma.					
33124956	8	33	theme	IL-1RI-IL-1RAcP	1300:1314	arg1	complex					1316:1322	IL-1RI-IL-1RAcP complex	1300:1322	IL-1RI-IL-1RAcP complex	1300:1322	Suggesting that the cytokine binding to IL-1RI is not required for the formation of IL-1RI-IL-1RAcP complex and the 'compact' complex could act as a down-regulatory mechanism.					
33124956	3	34	theme	protein	465:471	arg1	binding					418:424	binding	418:424	binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it	418:488	IL-1RI is activated upon formation of its functional assembly that occurs by binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it.					
33124956	3	35	theme	functional	383:392	arg1	assembly					394:401	its functional assembly	379:401	its functional assembly that occurs by binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it	379:488	IL-1RI is activated upon formation of its functional assembly that occurs by binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it.					
33124956	6	36	dep	classical	791:799	arg1	dynamics					837:844	molecular dynamics	827:844	molecular dynamics	827:844	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	7	37	dep	showed	983:988	arg1	abiding					1180:1186	both abiding	1175:1186	both abiding in the presence of glycans	1175:1213	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	1	38	theme	transmembrane	188:200	arg1	type					154:157	Interleukin 1 Receptor type I	131:159	Interleukin 1 Receptor type I (IL-1RI)	131:168	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	1	38	theme	transmembrane	188:200	arg1	receptor					202:209	a multi-domain transmembrane receptor	173:209	a multi-domain transmembrane receptor that triggers the inflammatory response	173:249	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	10	39	theme	functional	1619:1628	arg1	assembly					1630:1637	the IL-1RI functional assembly	1608:1637	the IL-1RI functional assembly	1608:1637	Taken together, it was shown that full glycosylation regulates formation of the IL-1RI functional assembly and play critical role in cytokine biding and triggering the IL-1RI involved downstream pathways in the cell.Communicated by Ramaswamy H. Sarma.					
33124956	4	40	theme	simulation	566:575	arg1	studies					577:583	simulation studies	566:583	simulation studies	566:583	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
33124956	7	41	theme	IL-1RI	1024:1029	arg1	form					1031:1034	the 'compact' and 'extended' IL-1RI form two types	995:1044	form	1031:1034	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	7	42	from	abiding	1180:1186	arg1	presence					1195:1202	the presence	1191:1202	the presence of glycans	1191:1213	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	4	43	theme	conformational	649:662	arg1	states					664:669	'compact' and 'extended' conformational states	624:669	'compact' and 'extended' conformational states	624:669	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
33124956	0	44	theme	IL-1RI	39:44	arg1	assembly					27:34	Glycan-mediated functional assembly	0:34	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.	0:129	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.					
33124956	4	45	theme	X-ray	526:530	arg1	Scattering					532:541	small-Angle X-ray Scattering	514:541	small-Angle X-ray Scattering	514:541	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
33124956	5	46	theme	activation	765:774	arg1	process					776:782	its activation process	761:782	its activation process	761:782	Furthermore, glycosylation has shown to play a critical role in its activation process.					
33124956	7	47	theme	compact	1000:1006	arg1	form					1031:1034	the 'compact' and 'extended' IL-1RI form two types	995:1044	form	1031:1034	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	3	48	theme	accessory	455:463	arg1	Il-1RAcP					474:481	Il-1RAcP	474:481	Il-1RAcP	474:481	IL-1RI is activated upon formation of its functional assembly that occurs by binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it.					
33124956	3	48	theme	accessory	455:463	arg1	protein					465:471	the accessory protein	451:471	the accessory protein (Il-1RAcP)	451:482	IL-1RI is activated upon formation of its functional assembly that occurs by binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it.					
33124956	7	49	theme	assemblies	1123:1132	arg1	form					1031:1034	the 'compact' and 'extended' IL-1RI form two types	995:1044	form	1031:1034	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	4	50	theme	small-Angle	514:524	arg1	Scattering					532:541	small-Angle X-ray Scattering	514:541	small-Angle X-ray Scattering	514:541	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
33124956	7	51	theme	extended	1014:1021	arg1	form					1031:1034	the 'compact' and 'extended' IL-1RI form two types	995:1044	form	1031:1034	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	6	52	theme	functional	950:959	arg1	assembly					961:968	the functional assembly	946:968	the functional assembly	946:968	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	9	53	theme	persistent	1453:1462	arg1	bonds					1473:1477	several persistent hydrogen bonds	1445:1477	several persistent hydrogen bonds between the IL-1RI-IL-1RAcP inter-connected glycans	1445:1529	The 'extended' complex is maintained by formation of several persistent hydrogen bonds between the IL-1RI-IL-1RAcP inter-connected glycans.					
33124956	0	54	theme	structural	47:56	arg1	insights					58:65	structural insights	47:65	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.	0:129	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.					
33124956	6	55	theme	IL-1RAcP	919:926	arg1	glycosylation					891:903	full glycosylation	886:903	full glycosylation of IL-1RI and IL-1RAcP	886:926	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	6	56	theme	IL-1RI	908:913	arg1	glycosylation					891:903	full glycosylation	886:903	full glycosylation of IL-1RI and IL-1RAcP	886:926	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	8	57	theme	down-regulatory	1365:1379	arg1	complex					1342:1348	the 'compact' complex	1328:1348	the 'compact' complex	1328:1348	Suggesting that the cytokine binding to IL-1RI is not required for the formation of IL-1RI-IL-1RAcP complex and the 'compact' complex could act as a down-regulatory mechanism.					
33124956	8	57	theme	down-regulatory	1365:1379	arg1	mechanism					1381:1389	a down-regulatory mechanism	1363:1389	a down-regulatory mechanism	1363:1389	Suggesting that the cytokine binding to IL-1RI is not required for the formation of IL-1RI-IL-1RAcP complex and the 'compact' complex could act as a down-regulatory mechanism.					
33124956	9	58	theme	hydrogen	1464:1471	arg1	bonds					1473:1477	several persistent hydrogen bonds	1445:1477	several persistent hydrogen bonds between the IL-1RI-IL-1RAcP inter-connected glycans	1445:1529	The 'extended' complex is maintained by formation of several persistent hydrogen bonds between the IL-1RI-IL-1RAcP inter-connected glycans.					
33124956	6	59	theme	molecular	827:835	arg1	dynamics					837:844	molecular dynamics	827:844	molecular dynamics	827:844	Here, classical and accelerated atomistic molecular dynamics were carried out to examine the role of full glycosylation of IL-1RI and IL-1RAcP in arrangement of the functional assembly.					
33124956	2	60	theme	detailed	270:277	arg1	mechanism					279:287	its detailed mechanism	266:287	its detailed mechanism of action	266:297	Understanding its detailed mechanism of action is crucial for treating immune disorders.					
33124956	1	61	theme	Interleukin	131:141	arg1	type					154:157	Interleukin 1 Receptor type I	131:159	Interleukin 1 Receptor type I (IL-1RI)	131:168	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	1	61	theme	Interleukin	131:141	arg1	IL-1RI					162:167	IL-1RI	162:167	IL-1RI	162:167	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	1	61	theme	Interleukin	131:141	arg1	receptor					202:209	a multi-domain transmembrane receptor	173:209	a multi-domain transmembrane receptor that triggers the inflammatory response	173:249	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	10	62	theme	downstream	1716:1725	arg1	pathways					1727:1734	the IL-1RI involved downstream pathways	1696:1734	the IL-1RI involved downstream pathways in the cell.Communicated	1696:1759	Taken together, it was shown that full glycosylation regulates formation of the IL-1RI functional assembly and play critical role in cytokine biding and triggering the IL-1RI involved downstream pathways in the cell.Communicated by Ramaswamy H. Sarma.					
33124956	0	63	dep	assembly	27:34	arg1	insights					58:65	structural insights	47:65	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.	0:129	Glycan-mediated functional assembly of IL-1RI: structural insights into completion of the current description for immune response.					
33124956	9	64	theme	IL-1RI-IL-1RAcP	1491:1505	arg1	glycans					1523:1529	the IL-1RI-IL-1RAcP inter-connected glycans	1487:1529	the IL-1RI-IL-1RAcP inter-connected glycans	1487:1529	The 'extended' complex is maintained by formation of several persistent hydrogen bonds between the IL-1RI-IL-1RAcP inter-connected glycans.					
33124956	8	65	theme	complex	1316:1322	arg1	formation					1287:1295	the formation	1283:1295	the formation of IL-1RI-IL-1RAcP complex	1283:1322	Suggesting that the cytokine binding to IL-1RI is not required for the formation of IL-1RI-IL-1RAcP complex and the 'compact' complex could act as a down-regulatory mechanism.					
33124956	9	66	theme	bonds	1473:1477	arg1	formation					1432:1440	formation	1432:1440	formation of several persistent hydrogen bonds between the IL-1RI-IL-1RAcP inter-connected glycans	1432:1529	The 'extended' complex is maintained by formation of several persistent hydrogen bonds between the IL-1RI-IL-1RAcP inter-connected glycans.					
33124956	7	67	theme	glycans	1207:1213	arg1	presence					1195:1202	the presence	1191:1202	the presence of glycans	1191:1213	Simulations showed that the 'compact' and 'extended' IL-1RI form two types of 'cytokine-inaccessible-non-signaling' and 'cytokine-accessible-signaling' assemblies with the IL-1RacP, respectively that are both abiding in the presence of glycans.					
33124956	9	68	theme	several	1445:1451	arg1	bonds					1473:1477	several persistent hydrogen bonds	1445:1477	several persistent hydrogen bonds between the IL-1RI-IL-1RAcP inter-connected glycans	1445:1529	The 'extended' complex is maintained by formation of several persistent hydrogen bonds between the IL-1RI-IL-1RAcP inter-connected glycans.					
33124956	1	69	theme	multi-domain	175:186	arg1	type					154:157	Interleukin 1 Receptor type I	131:159	Interleukin 1 Receptor type I (IL-1RI)	131:168	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	1	69	theme	multi-domain	175:186	arg1	receptor					202:209	a multi-domain transmembrane receptor	173:209	a multi-domain transmembrane receptor that triggers the inflammatory response	173:249	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	1	70	theme	Receptor	145:152	arg1	type					154:157	Interleukin 1 Receptor type I	131:159	Interleukin 1 Receptor type I (IL-1RI)	131:168	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	1	70	theme	Receptor	145:152	arg1	IL-1RI					162:167	IL-1RI	162:167	IL-1RI	162:167	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	1	70	theme	Receptor	145:152	arg1	receptor					202:209	a multi-domain transmembrane receptor	173:209	a multi-domain transmembrane receptor that triggers the inflammatory response	173:249	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	1	71	theme	inflammatory	229:240	arg1	response					242:249	the inflammatory response	225:249	the inflammatory response	225:249	Interleukin 1 Receptor type I (IL-1RI) is a multi-domain transmembrane receptor that triggers the inflammatory response.					
33124956	5	72	theme	critical	744:751	arg1	role					753:756	a critical role	742:756	a critical role	742:756	Furthermore, glycosylation has shown to play a critical role in its activation process.					
33124956	4	73	theme	X-ray	491:495	arg1	crystallography					497:511	X-ray crystallography	491:511	X-ray crystallography	491:511	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
33124956	10	74	theme	full	1566:1569	arg1	glycosylation					1571:1583	full glycosylation	1566:1583	full glycosylation	1566:1583	Taken together, it was shown that full glycosylation regulates formation of the IL-1RI functional assembly and play critical role in cytokine biding and triggering the IL-1RI involved downstream pathways in the cell.Communicated by Ramaswamy H. Sarma.					
33124956	3	75	theme	assembly	394:401	arg1	formation					366:374	formation	366:374	formation of its functional assembly that occurs by binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it	366:488	IL-1RI is activated upon formation of its functional assembly that occurs by binding of the IL-1 cytokine and the accessory protein (Il-1RAcP) to it.					
33124956	10	76	theme	IL-1RI	1612:1617	arg1	assembly					1630:1637	the IL-1RI functional assembly	1608:1637	the IL-1RI functional assembly	1608:1637	Taken together, it was shown that full glycosylation regulates formation of the IL-1RI functional assembly and play critical role in cytokine biding and triggering the IL-1RI involved downstream pathways in the cell.Communicated by Ramaswamy H. Sarma.					
33124956	4	77	dep	crystallography	497:511	arg1	studies					577:583	simulation studies	566:583	simulation studies	566:583	X-ray crystallography, small-Angle X-ray Scattering and molecular dynamics simulation studies showed that IL-1RI adopts two types of 'compact' and 'extended' conformational states in its dynamical pattern.					
34973728	4	0	theme	chitosan	999:1006	arg1	chains					1008:1013	the chitosan chains	995:1013	the chitosan chains	995:1013	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	3	1	theme	available	683:691	arg1	polymer					701:707	a readily available natural polymer	673:707	a readily available natural polymer	673:707	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	1	theme	available	683:691	arg1	chitosan					658:665	chitosan	658:665	chitosan (CS)	658:670	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	4	2	theme	acidic	878:883	arg1	conditions					885:894	acidic conditions	878:894	acidic conditions	878:894	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	1	3	theme	hydrogel	195:202	arg1	industry					204:211	the hydrogel industry	191:211	the hydrogel industry	191:211	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	1	4	theme	electromagnetic	356:370	arg1	fields					372:377	electromagnetic fields	356:377	electromagnetic fields	356:377	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	1	5	theme	industry	204:211	arg1	attention					178:186	the attention	174:186	the attention of the hydrogel industry	174:211	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	3	6	theme	packed	560:565	arg1	CNFs					589:592	CNFs	589:592	CNFs	589:592	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	6	theme	packed	560:565	arg1	nanofibers					577:586	tightly packed cellulose nanofibers	552:586	tightly packed cellulose nanofibers (CNFs)	552:593	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	7	with	hydrogel	538:545	arg1	CNFs					589:592	CNFs	589:592	CNFs	589:592	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	7	with	hydrogel	538:545	arg1	nanofibers					577:586	tightly packed cellulose nanofibers	552:586	tightly packed cellulose nanofibers (CNFs)	552:593	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	8	theme	cellulose	567:575	arg1	CNFs					589:592	CNFs	589:592	CNFs	589:592	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	8	theme	cellulose	567:575	arg1	nanofibers					577:586	tightly packed cellulose nanofibers	552:586	tightly packed cellulose nanofibers (CNFs)	552:593	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	1	9	theme	external	298:305	arg1	temperature					327:337	temperature	327:337	temperature	327:337	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	1	9	theme	external	298:305	arg1	stimuli					307:313	external stimuli	298:313	external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light	298:388	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	1	9	theme	external	298:305	arg1	light					384:388	light	384:388	light	384:388	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	1	9	theme	external	298:305	arg1	strength					346:353	ionic strength	340:353	ionic strength	340:353	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	1	9	theme	external	298:305	arg1	pH					323:324	pH	323:324	pH	323:324	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	1	9	theme	external	298:305	arg1	fields					372:377	electromagnetic fields	356:377	electromagnetic fields	356:377	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	4	10	theme	amino	979:983	arg1	chains					1008:1013	the chitosan chains	995:1013	the chitosan chains	995:1013	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	4	10	theme	amino	979:983	arg1	groups					985:990	amino groups	979:990	amino groups of the chitosan chains	979:1013	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	5	11	theme	composites	1112:1121	arg1	strength					1096:1103	the mechanical strength	1081:1103	the mechanical strength of the composites	1081:1121	Osmotic pressure is built up under acidic conditions, increasing the mechanical strength of the composites.					
34973728	4	12	theme	higher	843:848	arg1	properties					861:870	higher mechanical properties	843:870	higher mechanical properties under acidic conditions	843:894	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	2	13	theme	hydrogel	419:426	arg1	applications					428:439	stimuli-responsive hydrogel applications	400:439	stimuli-responsive hydrogel applications	400:439	However, stimuli-responsive hydrogel applications are hindered due to their inevitable swelling and shrinkage.					
34973728	3	14	theme	natural	693:699	arg1	polymer					701:707	a readily available natural polymer	673:707	a readily available natural polymer	673:707	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	14	theme	natural	693:699	arg1	chitosan					658:665	chitosan	658:665	chitosan (CS)	658:670	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	0	15	theme	Stimuli-responsive	0:17	arg1	hydrogels					29:37	Stimuli-responsive composite hydrogels	0:37	Stimuli-responsive composite hydrogels with three-dimensional stability	0:70	Stimuli-responsive composite hydrogels with three-dimensional stability prepared using oxidized cellulose nanofibers and chitosan.					
34973728	2	16	theme	stimuli-responsive	400:417	arg1	applications					428:439	stimuli-responsive hydrogel applications	400:439	stimuli-responsive hydrogel applications	400:439	However, stimuli-responsive hydrogel applications are hindered due to their inevitable swelling and shrinkage.					
34973728	0	17	theme	composite	19:27	arg1	hydrogels					29:37	Stimuli-responsive composite hydrogels	0:37	Stimuli-responsive composite hydrogels with three-dimensional stability	0:70	Stimuli-responsive composite hydrogels with three-dimensional stability prepared using oxidized cellulose nanofibers and chitosan.					
34973728	2	18	theme	inevitable	467:476	arg1	swelling					478:485	their inevitable swelling	461:485	their inevitable swelling	461:485	However, stimuli-responsive hydrogel applications are hindered due to their inevitable swelling and shrinkage.					
34973728	4	19	theme	mechanical	850:859	arg1	properties					861:870	higher mechanical properties	843:870	higher mechanical properties under acidic conditions	843:894	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	3	20	theme	adaptive	736:743	arg1	composite					754:762	a mechanically adaptive hydrogel composite	721:762	a mechanically adaptive hydrogel composite	721:762	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	1	21	theme	Stimuli-responsive	131:148	arg1	hydrogels					150:158	Stimuli-responsive hydrogels	131:158	Stimuli-responsive hydrogels	131:158	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	4	22	theme	basic	934:938	arg1	conditions					940:949	basic conditions	934:949	basic conditions owing to the protonation of amino groups of the chitosan chains	934:1013	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	6	23	theme	composites	1164:1173	arg1	stability					1151:1159	The good three-dimensional stability	1124:1159	The good three-dimensional stability of composites	1124:1173	The good three-dimensional stability of composites enables them to consistently maintain their volume when exposed to acidic or basic conditions.					
34973728	3	24	theme	Bacterial	502:510	arg1	BC					523:524	BC	523:524	BC	523:524	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	24	theme	Bacterial	502:510	arg1	cellulose					512:520	Bacterial cellulose	502:520	Bacterial cellulose (BC)	502:525	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	24	theme	Bacterial	502:510	arg1	hydrogel					538:545	a natural hydrogel	528:545	a natural hydrogel with tightly packed cellulose nanofibers (CNFs)	528:593	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	5	25	theme	mechanical	1085:1094	arg1	strength					1096:1103	the mechanical strength	1081:1103	the mechanical strength of the composites	1081:1121	Osmotic pressure is built up under acidic conditions, increasing the mechanical strength of the composites.					
34973728	4	26	theme	pH	814:815	arg1	sensitivity					817:827	pH sensitivity	814:827	pH sensitivity	814:827	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	0	27	theme	three-dimensional	44:60	arg1	stability					62:70	three-dimensional stability	44:70	three-dimensional stability	44:70	Stimuli-responsive composite hydrogels with three-dimensional stability prepared using oxidized cellulose nanofibers and chitosan.					
34973728	4	28	theme	chains	1008:1013	arg1	chains					1008:1013	the chitosan chains	995:1013	the chitosan chains	995:1013	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	4	28	theme	chains	1008:1013	arg1	groups					985:990	amino groups	979:990	amino groups of the chitosan chains	979:1013	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	1	29	theme	chemical	260:267	arg1	properties					269:278	their physical and chemical properties	241:278	their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light	241:388	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	3	30	theme	different	770:778	arg1	conditions					783:792	different pH conditions	770:792	different pH conditions	770:792	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	4	31	theme	mechanical	906:915	arg1	properties					917:926	lower mechanical properties	900:926	lower mechanical properties	900:926	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	4	32	theme	groups	985:990	arg1	protonation					964:974	the protonation	960:974	the protonation of amino groups of the chitosan chains	960:1013	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	6	33	theme	basic	1252:1256	arg1	conditions					1258:1267	acidic or basic conditions	1242:1267	acidic or basic conditions	1242:1267	The good three-dimensional stability of composites enables them to consistently maintain their volume when exposed to acidic or basic conditions.					
34973728	0	34	with	hydrogels	29:37	arg1	stability					62:70	three-dimensional stability	44:70	three-dimensional stability	44:70	Stimuli-responsive composite hydrogels with three-dimensional stability prepared using oxidized cellulose nanofibers and chitosan.					
34973728	3	35	theme	hydrogel	745:752	arg1	composite					754:762	a mechanically adaptive hydrogel composite	721:762	a mechanically adaptive hydrogel composite	721:762	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	4	36	theme	lower	900:904	arg1	properties					917:926	lower mechanical properties	900:926	lower mechanical properties	900:926	Composites exhibit pH sensitivity by presenting higher mechanical properties under acidic conditions and lower mechanical properties under basic conditions owing to the protonation of amino groups of the chitosan chains.					
34973728	5	37	theme	acidic	1051:1056	arg1	conditions					1058:1067	acidic conditions	1051:1067	acidic conditions	1051:1067	Osmotic pressure is built up under acidic conditions, increasing the mechanical strength of the composites.					
34973728	1	38	theme	ionic	340:344	arg1	strength					346:353	ionic strength	340:353	ionic strength	340:353	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	6	39	theme	acidic	1242:1247	arg1	conditions					1258:1267	acidic or basic conditions	1242:1267	acidic or basic conditions	1242:1267	The good three-dimensional stability of composites enables them to consistently maintain their volume when exposed to acidic or basic conditions.					
34973728	3	40	theme	dialdehyde	613:622	arg1	DABC					628:631	DABC	628:631	DABC	628:631	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	40	theme	dialdehyde	613:622	arg1	BC					624:625	dialdehyde BC	613:625	dialdehyde BC (DABC)	613:632	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	6	41	theme	three-dimensional	1133:1149	arg1	stability					1151:1159	The good three-dimensional stability	1124:1159	The good three-dimensional stability of composites	1124:1173	The good three-dimensional stability of composites enables them to consistently maintain their volume when exposed to acidic or basic conditions.					
34973728	1	42	theme	physical	247:254	arg1	properties					269:278	their physical and chemical properties	241:278	their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light	241:388	Stimuli-responsive hydrogels have garnered the attention of the hydrogel industry, as they are able to change their physical and chemical properties based on changing external stimuli such as pH, temperature, ionic strength, electromagnetic fields, and light.					
34973728	6	43	theme	good	1128:1131	arg1	stability					1151:1159	The good three-dimensional stability	1124:1159	The good three-dimensional stability of composites	1124:1173	The good three-dimensional stability of composites enables them to consistently maintain their volume when exposed to acidic or basic conditions.					
34973728	0	44	theme	cellulose	96:104	arg1	nanofibers					106:115	oxidized cellulose nanofibers	87:115	oxidized cellulose nanofibers	87:115	Stimuli-responsive composite hydrogels with three-dimensional stability prepared using oxidized cellulose nanofibers and chitosan.					
34973728	5	45	theme	Osmotic	1016:1022	arg1	pressure					1024:1031	Osmotic pressure	1016:1031	Osmotic pressure	1016:1031	Osmotic pressure is built up under acidic conditions, increasing the mechanical strength of the composites.					
34973728	3	46	theme	natural	530:536	arg1	cellulose					512:520	Bacterial cellulose	502:520	Bacterial cellulose (BC)	502:525	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	46	theme	natural	530:536	arg1	hydrogel					538:545	a natural hydrogel	528:545	a natural hydrogel with tightly packed cellulose nanofibers (CNFs)	528:593	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	3	47	theme	pH	780:781	arg1	conditions					783:792	different pH conditions	770:792	different pH conditions	770:792	Bacterial cellulose (BC), a natural hydrogel with tightly packed cellulose nanofibers (CNFs) was oxidized into dialdehyde BC (DABC) and was composited with chitosan (CS), a readily available natural polymer, to develop a mechanically adaptive hydrogel composite under different pH conditions.					
34973728	0	48	theme	oxidized	87:94	arg1	nanofibers					106:115	oxidized cellulose nanofibers	87:115	oxidized cellulose nanofibers	87:115	Stimuli-responsive composite hydrogels with three-dimensional stability prepared using oxidized cellulose nanofibers and chitosan.					
33360415	2	0	theme	variants	512:519	arg1	fractions					475:483	fractions	475:483	fractions of acidic and basic charge variants	475:519	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	2	1	theme	biopharmaceutical	369:385	arg1	facility					387:394	a biopharmaceutical facility	367:394	a biopharmaceutical facility	367:394	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	6	2	theme	inhibitory	1008:1017	arg1	activity					1019:1026	cell death inhibitory activity	997:1026	cell death inhibitory activity	997:1026	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	2	3	theme	charge	505:510	arg1	variants					512:519	acidic and basic charge variants	488:519	acidic and basic charge variants	488:519	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	1	4	theme	routine	161:167	arg1	manufacturing					169:181	the routine manufacturing	157:181	the routine manufacturing of monoclonal antibodies	157:206	Charge variants are the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies.					
33360415	6	5	theme	death	1002:1006	arg1	activity					1019:1026	cell death inhibitory activity	997:1026	cell death inhibitory activity	997:1026	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	6	6	theme	biological	1150:1159	arg1	functions					1161:1169	biological functions	1150:1169	biological functions	1150:1169	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	6	7	theme	cell	997:1000	arg1	activity					1019:1026	cell death inhibitory activity	997:1026	cell death inhibitory activity	997:1026	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	7	8	theme	variants	1223:1230	arg1	understanding					1199:1211	the understanding	1195:1211	the understanding of charge variants, which are usually effective components that should not be intentionally reduced unless biological functions are affected	1195:1352	This research enhances the understanding of charge variants, which are usually effective components that should not be intentionally reduced unless biological functions are affected.					
33360415	4	9	theme	higher	768:773	arg1	levels					775:780	higher levels	768:780	higher levels of sialylation and galactosylation of N-linked glycans	768:835	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	10	theme	Met	736:738	arg1	oxidation					740:748	Met oxidation	736:748	Met oxidation	736:748	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	2	11	theme	main	539:542	arg1	species					544:550	the main species	535:550	the main species	535:550	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	4	12	theme	sialylation	785:795	arg1	deamidation					755:765	Asn deamidation	751:765	Asn deamidation	751:765	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	12	theme	sialylation	785:795	arg1	levels					775:780	higher levels	768:780	higher levels of sialylation and galactosylation of N-linked glycans	768:835	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	12	theme	sialylation	785:795	arg1	mannose					852:858	less high mannose	842:858	less high mannose	842:858	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	12	theme	sialylation	785:795	arg1	fragments					725:733	fragments	725:733	fragments	725:733	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	12	theme	sialylation	785:795	arg1	oxidation					740:748	Met oxidation	736:748	Met oxidation	736:748	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	2	13	from	insight	225:231	arg1	functions					320:328	biological functions	309:328	biological functions	309:328	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	2	14	theme	cation	428:433	arg1	chromatography					444:457	semipreparative cation exchange chromatography	412:457	semipreparative cation exchange chromatography (CEX)	412:463	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	2	14	theme	cation	428:433	arg1	CEX					460:462	CEX	460:462	CEX	460:462	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	2	15	theme	basic	499:503	arg1	variants					512:519	acidic and basic charge variants	488:519	acidic and basic charge variants	488:519	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	1	16	from	sources	129:135	arg1	manufacturing					169:181	the routine manufacturing	157:181	the routine manufacturing of monoclonal antibodies	157:206	Charge variants are the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies.					
33360415	1	17	theme	Charge	82:87	arg1	sources					129:135	the most commonly observed sources	102:135	the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies	102:206	Charge variants are the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies.					
33360415	1	17	theme	Charge	82:87	arg1	variants					89:96	Charge variants	82:96	Charge variants	82:96	Charge variants are the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies.					
33360415	6	18	theme	FcγRIIIa	1061:1068	arg1	analysis					959:966	further analysis	951:966	further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity	951:1086	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	2	19	theme	semipreparative	412:426	arg1	chromatography					444:457	semipreparative cation exchange chromatography	412:457	semipreparative cation exchange chromatography (CEX)	412:463	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	2	19	theme	semipreparative	412:426	arg1	CEX					460:462	CEX	460:462	CEX	460:462	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	4	20	gly	sialylation	785:795	arg1	glycans					829:835	N-linked glycans	820:835	N-linked glycans	820:835	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	21	theme	Asn	751:753	arg1	deamidation					755:765	Asn deamidation	751:765	Asn deamidation	751:765	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	1	22	theme	monoclonal	186:195	arg1	antibodies					197:206	monoclonal antibodies	186:206	monoclonal antibodies	186:206	Charge variants are the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies.					
33360415	6	23	theme	affinity	987:994	arg1	analysis					959:966	further analysis	951:966	further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity	951:1086	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	5	24	theme	basic	865:869	arg1	variants					871:878	The basic variants	861:878	The basic variants	861:878	The basic variants resulted mainly from aggregates, fragments, and Met oxidation.					
33360415	6	25	theme	charge	1114:1119	arg1	heterogeneity					1121:1133	the charge heterogeneity	1110:1133	the charge heterogeneity	1110:1133	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	1	26	theme	antibodies	197:206	arg1	manufacturing					169:181	the routine manufacturing	157:181	the routine manufacturing of monoclonal antibodies	157:206	Charge variants are the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies.					
33360415	6	27	theme	binding	979:985	arg1	affinity					987:994	antigen binding affinity	971:994	antigen binding affinity	971:994	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	2	28	from	facility	387:394	arg1	HS626					356:360	an infliximab biosimilar HS626	331:360	an infliximab biosimilar HS626 from a biopharmaceutical facility	331:394	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	6	29	theme	FcRn	1055:1058	arg1	analysis					959:966	further analysis	951:966	further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity	951:1086	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	4	30	link	N-linked	820:827	arg1	glycans					829:835	N-linked glycans	820:835	N-linked glycans	820:835	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	6	31	theme	antigen	971:977	arg1	affinity					987:994	antigen binding affinity	971:994	antigen binding affinity	971:994	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	2	32	theme	biological	309:318	arg1	functions					320:328	biological functions	309:328	biological functions	309:328	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	4	33	theme	physicochemical	637:651	arg1	characterizations					653:669	structural and physicochemical characterizations	622:669	structural and physicochemical characterizations	622:669	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	0	34	theme	variants	41:48	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of charge variants of infliximab biosimilar HS626.					
33360415	0	34	theme	variants	41:48	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of charge variants of infliximab biosimilar HS626.					
33360415	4	35	theme	acidic	694:699	arg1	variants					701:708	the acidic variants	690:708	the acidic variants	690:708	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	2	36	theme	structural	242:251	arg1	foundation					253:262	the structural foundation	238:262	the structural foundation of charge heterogeneity	238:286	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	6	37	theme	CDC	1039:1041	arg1	analysis					959:966	further analysis	951:966	further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity	951:1086	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	0	38	theme	charge	34:39	arg1	variants					41:48	charge variants	34:48	charge variants of infliximab biosimilar HS626	34:79	Isolation and characterization of charge variants of infliximab biosimilar HS626.					
33360415	4	39	theme	glycans	829:835	arg1	galactosylation					801:815	galactosylation	801:815	galactosylation	801:815	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	39	theme	glycans	829:835	arg1	sialylation					785:795	sialylation	785:795	sialylation	785:795	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	2	40	theme	exchange	435:442	arg1	chromatography					444:457	semipreparative cation exchange chromatography	412:457	semipreparative cation exchange chromatography (CEX)	412:463	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	2	40	theme	exchange	435:442	arg1	CEX					460:462	CEX	460:462	CEX	460:462	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	0	41	theme	infliximab	53:62	arg1	biosimilar					64:73	infliximab biosimilar HS626	53:79	infliximab biosimilar HS626	53:79	Isolation and characterization of charge variants of infliximab biosimilar HS626.					
33360415	6	42	theme	further	951:957	arg1	analysis					959:966	further analysis	951:966	further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity	951:1086	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	0	43	theme	biosimilar	64:73	arg1	variants					41:48	charge variants	34:48	charge variants of infliximab biosimilar HS626	34:79	Isolation and characterization of charge variants of infliximab biosimilar HS626.					
33360415	6	44	theme	C1q	1075:1077	arg1	analysis					959:966	further analysis	951:966	further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity	951:1086	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	7	45	theme	effective	1251:1259	arg1	variants					1223:1230	charge variants	1216:1230	charge variants	1216:1230	This research enhances the understanding of charge variants, which are usually effective components that should not be intentionally reduced unless biological functions are affected.					
33360415	7	45	theme	effective	1251:1259	arg1	components					1261:1270	effective components	1251:1270	effective components that should not be intentionally reduced unless biological functions are affected	1251:1352	This research enhances the understanding of charge variants, which are usually effective components that should not be intentionally reduced unless biological functions are affected.					
33360415	2	46	theme	further	217:223	arg1	insight					225:231	further insight	217:231	further insight into the structural foundation of charge heterogeneity	217:286	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	4	47	theme	high	847:850	arg1	mannose					852:858	less high mannose	842:858	less high mannose	842:858	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	7	48	theme	biological	1320:1329	arg1	functions					1331:1339	biological functions	1320:1339	biological functions	1320:1339	This research enhances the understanding of charge variants, which are usually effective components that should not be intentionally reduced unless biological functions are affected.					
33360415	1	49	theme	observed	120:127	arg1	sources					129:135	the most commonly observed sources	102:135	the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies	102:206	Charge variants are the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies.					
33360415	1	49	theme	observed	120:127	arg1	variants					89:96	Charge variants	82:96	Charge variants	82:96	Charge variants are the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies.					
33360415	4	50	theme	galactosylation	801:815	arg1	deamidation					755:765	Asn deamidation	751:765	Asn deamidation	751:765	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	50	theme	galactosylation	801:815	arg1	levels					775:780	higher levels	768:780	higher levels of sialylation and galactosylation of N-linked glycans	768:835	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	50	theme	galactosylation	801:815	arg1	mannose					852:858	less high mannose	842:858	less high mannose	842:858	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	50	theme	galactosylation	801:815	arg1	fragments					725:733	fragments	725:733	fragments	725:733	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	50	theme	galactosylation	801:815	arg1	oxidation					740:748	Met oxidation	736:748	Met oxidation	736:748	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	2	51	theme	heterogeneity	274:286	arg1	foundation					253:262	the structural foundation	238:262	the structural foundation of charge heterogeneity	238:286	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	6	52	theme	ADCC	1029:1032	arg1	analysis					959:966	further analysis	951:966	further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity	951:1086	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	2	53	theme	biosimilar	345:354	arg1	HS626					356:360	an infliximab biosimilar HS626	331:360	an infliximab biosimilar HS626 from a biopharmaceutical facility	331:394	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	6	54	dep	affinity	987:994	arg1	affinity					1079:1086	affinity	1079:1086	affinity	1079:1086	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	2	55	theme	charge	267:272	arg1	heterogeneity					274:286	charge heterogeneity	267:286	charge heterogeneity	267:286	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	2	56	theme	infliximab	334:343	arg1	HS626					356:360	an infliximab biosimilar HS626	331:360	an infliximab biosimilar HS626 from a biopharmaceutical facility	331:394	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	5	57	theme	Met	928:930	arg1	oxidation					932:940	Met oxidation	928:940	Met oxidation	928:940	The basic variants resulted mainly from aggregates, fragments, and Met oxidation.					
33360415	4	58	theme	N-linked	820:827	arg1	glycans					829:835	N-linked glycans	820:835	N-linked glycans	820:835	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	4	59	theme	characterizations	653:669	arg1	series					612:617	a series	610:617	a series of structural and physicochemical characterizations	610:669	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33360415	1	60	theme	heterogeneity	140:152	arg1	sources					129:135	the most commonly observed sources	102:135	the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies	102:206	Charge variants are the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies.					
33360415	1	60	theme	heterogeneity	140:152	arg1	variants					89:96	Charge variants	82:96	Charge variants	82:96	Charge variants are the most commonly observed sources of heterogeneity in the routine manufacturing of monoclonal antibodies.					
33360415	2	61	from	influence	296:304	arg1	functions					320:328	biological functions	309:328	biological functions	309:328	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	2	62	theme	acidic	488:493	arg1	variants					512:519	acidic and basic charge variants	488:519	acidic and basic charge variants	488:519	To gain further insight into the structural foundation of charge heterogeneity and its influence on biological functions, an infliximab biosimilar HS626 from a biopharmaceutical facility was isolated by semipreparative cation exchange chromatography (CEX) to obtain fractions of acidic and basic charge variants and determine the main species.					
33360415	6	63	theme	activity	1019:1026	arg1	analysis					959:966	further analysis	951:966	further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity	951:1086	Through further analysis of antigen binding affinity, cell death inhibitory activity, ADCC, and CDC, as well as FcRn, FcγRIIIa, and C1q affinity, we demonstrated that the charge heterogeneity did not affect biological functions.					
33360415	7	64	theme	charge	1216:1221	arg1	variants					1223:1230	charge variants	1216:1230	charge variants	1216:1230	This research enhances the understanding of charge variants, which are usually effective components that should not be intentionally reduced unless biological functions are affected.					
33360415	7	64	theme	charge	1216:1221	arg1	components					1261:1270	effective components	1251:1270	effective components that should not be intentionally reduced unless biological functions are affected	1251:1352	This research enhances the understanding of charge variants, which are usually effective components that should not be intentionally reduced unless biological functions are affected.					
33360415	4	65	theme	structural	622:631	arg1	characterizations					653:669	structural and physicochemical characterizations	622:669	structural and physicochemical characterizations	622:669	Through a series of structural and physicochemical characterizations, we concluded that the acidic variants were caused by fragments, Met oxidation, Asn deamidation, higher levels of sialylation and galactosylation of N-linked glycans, and less high mannose.					
33808330	3	0	theme	regioselective	557:570	arg1	glycosylation					572:584	regioselective glycosylation	557:584	regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment	557:638	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	1	1	theme	13,28-epoxy-ether	122:138	arg1	moiety					140:145	a unique 13,28-epoxy-ether moiety	113:145	a unique 13,28-epoxy-ether moiety	113:145	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	3	2	theme	-catalyzed	662:671	arg1	glycosylation					673:685	efficient gold(I)-catalyzed glycosylation	645:685	efficient gold(I)-catalyzed glycosylation	645:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	2	3	theme	saikosaponin	381:392	arg1	5					397:397	5	397:397	5	397:397	Herein, we report the first synthesis of saikosaponin A/D (1-2) and their natural congeners, including prosaikosaponin F (3), G (4), saikosaponin Y (5), prosaikogenin (6), and clinoposaponin I (7).					
33808330	2	3	theme	saikosaponin	381:392	arg1	Y					394:394	saikosaponin Y	381:394	saikosaponin Y (5)	381:398	Herein, we report the first synthesis of saikosaponin A/D (1-2) and their natural congeners, including prosaikosaponin F (3), G (4), saikosaponin Y (5), prosaikogenin (6), and clinoposaponin I (7).					
33808330	3	4	theme	oleanolic	541:549	arg1	acid					551:554	oleanolic acid	541:554	oleanolic acid	541:554	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	1	5	theme	biological	205:214	arg1	activities					236:245	biological and pharmacological activities	205:245	biological and pharmacological activities	205:245	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	1	6	theme	pharmacological	220:234	arg1	activities					236:245	biological and pharmacological activities	205:245	biological and pharmacological activities	205:245	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	3	7	from	aglycones	502:510	arg1	acid					551:554	oleanolic acid	541:554	oleanolic acid	541:554	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	7	from	aglycones	502:510	arg1	glycosylation					572:584	regioselective glycosylation	557:584	regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment	557:638	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	7	from	aglycones	502:510	arg1	glycosylation					673:685	efficient gold(I)-catalyzed glycosylation	645:685	efficient gold(I)-catalyzed glycosylation	645:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	1	8	theme	typical	72:78	arg1	saponins					94:101	typical oleanane-type saponins	72:101	typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities	72:245	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	1	8	theme	typical	72:78	arg1	D					60:60	D	60:60	D (SSd)	60:66	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	1	8	theme	typical	72:78	arg1	A					48:48	Saikosaponin A	35:48	Saikosaponin A (SSa)	35:54	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	1	9	theme	D	150:150	arg1	ring					152:155	D ring	150:155	D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities	150:245	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	3	10	from	glycosylation	572:584	arg1	aglycones					502:510	the aglycones	498:510	the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	498:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	10	from	glycosylation	572:584	arg1	state					530:534	high oxidation state	515:534	high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	515:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	2	11	theme	first	270:274	arg1	synthesis					276:284	the first synthesis	266:284	the first synthesis of saikosaponin A/D (1-2) and their natural congeners	266:338	Herein, we report the first synthesis of saikosaponin A/D (1-2) and their natural congeners, including prosaikosaponin F (3), G (4), saikosaponin Y (5), prosaikogenin (6), and clinoposaponin I (7).					
33808330	2	12	theme	natural	322:328	arg1	congeners					330:338	their natural congeners	316:338	their natural congeners	316:338	Herein, we report the first synthesis of saikosaponin A/D (1-2) and their natural congeners, including prosaikosaponin F (3), G (4), saikosaponin Y (5), prosaikogenin (6), and clinoposaponin I (7).					
33808330	0	13	theme	Facile	0:5	arg1	Synthesis					7:15	Facile Synthesis	0:15	Facile Synthesis of Saikosaponins.	0:33	Facile Synthesis of Saikosaponins.					
33808330	1	14	theme	activities	236:245	arg1	range					196:200	a wide range	189:200	a wide range of biological and pharmacological activities	189:245	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	3	15	dep	glycosylation	673:685	arg1	I					660:660	I	660:660	I	660:660	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	0	16	theme	Saikosaponins	20:32	arg1	Synthesis					7:15	Facile Synthesis	0:15	Facile Synthesis of Saikosaponins.	0:33	Facile Synthesis of Saikosaponins.					
33808330	3	17	theme	aglycones	502:510	arg1	preparation					483:493	ready preparation	477:493	ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	477:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	1	18	theme	aglycones	164:172	arg1	ring					152:155	D ring	150:155	D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities	150:245	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	2	19	theme	saikosaponin	289:300	arg1	1-2					307:309	1-2	307:309	1-2	307:309	Herein, we report the first synthesis of saikosaponin A/D (1-2) and their natural congeners, including prosaikosaponin F (3), G (4), saikosaponin Y (5), prosaikogenin (6), and clinoposaponin I (7).					
33808330	2	19	theme	saikosaponin	289:300	arg1	A/D					302:304	saikosaponin A/D	289:304	saikosaponin A/D (1-2)	289:310	Herein, we report the first synthesis of saikosaponin A/D (1-2) and their natural congeners, including prosaikosaponin F (3), G (4), saikosaponin Y (5), prosaikogenin (6), and clinoposaponin I (7).					
33808330	2	20	theme	prosaikosaponin	351:365	arg1	3					370:370	3	370:370	3	370:370	Herein, we report the first synthesis of saikosaponin A/D (1-2) and their natural congeners, including prosaikosaponin F (3), G (4), saikosaponin Y (5), prosaikogenin (6), and clinoposaponin I (7).					
33808330	2	20	theme	prosaikosaponin	351:365	arg1	F					367:367	prosaikosaponin F	351:367	prosaikosaponin F (3)	351:371	Herein, we report the first synthesis of saikosaponin A/D (1-2) and their natural congeners, including prosaikosaponin F (3), G (4), saikosaponin Y (5), prosaikogenin (6), and clinoposaponin I (7).					
33808330	3	21	from	acid	551:554	arg1	aglycones					502:510	the aglycones	498:510	the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	498:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	21	from	acid	551:554	arg1	state					530:534	high oxidation state	515:534	high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	515:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	22	from	glycosylation	673:685	arg1	aglycones					502:510	the aglycones	498:510	the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	498:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	22	from	glycosylation	673:685	arg1	state					530:534	high oxidation state	515:534	high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	515:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	23	theme	present	450:456	arg1	synthesis					458:466	The present synthesis	446:466	The present synthesis	446:466	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	24	link	-linked	610:616	arg1	fragment					631:638	the β-(1→3)-linked disaccharide fragment	599:638	the β-(1→3)-linked disaccharide fragment	599:638	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	2	25	theme	A/D	302:304	arg1	synthesis					276:284	the first synthesis	266:284	the first synthesis of saikosaponin A/D (1-2) and their natural congeners	266:338	Herein, we report the first synthesis of saikosaponin A/D (1-2) and their natural congeners, including prosaikosaponin F (3), G (4), saikosaponin Y (5), prosaikogenin (6), and clinoposaponin I (7).					
33808330	3	26	theme	-linked	610:616	arg1	fragment					631:638	the β-(1→3)-linked disaccharide fragment	599:638	the β-(1→3)-linked disaccharide fragment	599:638	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	27	theme	state	530:534	arg1	aglycones					502:510	the aglycones	498:510	the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	498:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	1	28	theme	oleanane-type	80:92	arg1	saponins					94:101	typical oleanane-type saponins	72:101	typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities	72:245	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	1	28	theme	oleanane-type	80:92	arg1	D					60:60	D	60:60	D (SSd)	60:66	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	1	28	theme	oleanane-type	80:92	arg1	A					48:48	Saikosaponin A	35:48	Saikosaponin A (SSa)	35:54	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	3	29	theme	disaccharide	618:629	arg1	fragment					631:638	the β-(1→3)-linked disaccharide fragment	599:638	the β-(1→3)-linked disaccharide fragment	599:638	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	30	theme	ready	477:481	arg1	preparation					483:493	ready preparation	477:493	ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	477:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	31	theme	gold	655:658	arg1	glycosylation					673:685	efficient gold(I)-catalyzed glycosylation	645:685	efficient gold(I)-catalyzed glycosylation	645:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	3	32	theme	high	515:518	arg1	state					530:534	high oxidation state	515:534	high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	515:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	1	33	theme	Saikosaponin	35:46	arg1	saponins					94:101	typical oleanane-type saponins	72:101	typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities	72:245	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	1	33	theme	Saikosaponin	35:46	arg1	SSa					51:53	SSa	51:53	SSa	51:53	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	1	33	theme	Saikosaponin	35:46	arg1	D					60:60	D	60:60	D (SSd)	60:66	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	1	33	theme	Saikosaponin	35:46	arg1	A					48:48	Saikosaponin A	35:48	Saikosaponin A (SSa)	35:54	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	3	34	theme	oxidation	520:528	arg1	state					530:534	high oxidation state	515:534	high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation	515:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
33808330	2	35	theme	congeners	330:338	arg1	synthesis					276:284	the first synthesis	266:284	the first synthesis of saikosaponin A/D (1-2) and their natural congeners	266:338	Herein, we report the first synthesis of saikosaponin A/D (1-2) and their natural congeners, including prosaikosaponin F (3), G (4), saikosaponin Y (5), prosaikogenin (6), and clinoposaponin I (7).					
33808330	1	36	theme	wide	191:194	arg1	range					196:200	a wide range	189:200	a wide range of biological and pharmacological activities	189:245	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	1	37	theme	unique	115:120	arg1	moiety					140:145	a unique 13,28-epoxy-ether moiety	113:145	a unique 13,28-epoxy-ether moiety	113:145	Saikosaponin A (SSa) and D (SSd) are typical oleanane-type saponins featuring a unique 13,28-epoxy-ether moiety at D ring of the aglycones, which exhibit a wide range of biological and pharmacological activities.					
33808330	3	38	theme	efficient	645:653	arg1	glycosylation					673:685	efficient gold(I)-catalyzed glycosylation	645:685	efficient gold(I)-catalyzed glycosylation	645:685	The present synthesis features ready preparation of the aglycones of high oxidation state from oleanolic acid, regioselective glycosylation to construct the β-(1→3)-linked disaccharide fragment, and efficient gold(I)-catalyzed glycosylation to install the glycans on to the aglycones.					
32959924	4	0	theme	GLMP	681:684	arg1	N-glycosylation					662:676	N-glycosylation	662:676	N-glycosylation of GLMP in general	662:695	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	4	1	dep	N-glycans	718:726	arg1	complex-type					750:761	complex-type	750:761	complex-type	750:761	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	4	1	dep	N-glycans	718:726	arg1	high-mannose-type					729:745	high-mannose-type	729:745	high-mannose-type	729:745	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	4	2	from	N-glycosylation	662:676	arg1	general					689:695	general	689:695	general	689:695	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	1	3	theme	lysosomal	250:258	arg1	proteolysis					260:270	lysosomal proteolysis	250:270	lysosomal proteolysis	250:270	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	0	4	theme	subunit	94:100	arg1	GLMP					102:105	its accessory subunit GLMP	80:105	its accessory subunit GLMP	80:105	Characterization of the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP.					
32959924	0	5	dep	transporter	58:68	arg1	GLMP					102:105	its accessory subunit GLMP	80:105	its accessory subunit GLMP	80:105	Characterization of the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP.					
32959924	0	5	dep	transporter	58:68	arg1	MFSD1					70:74	MFSD1	70:74	MFSD1	70:74	Characterization of the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP.					
32959924	0	6	theme	accessory	84:92	arg1	GLMP					102:105	its accessory subunit GLMP	80:105	its accessory subunit GLMP	80:105	Characterization of the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP.					
32959924	8	7	dep	intimate	1227:1234	arg1	couple					1236:1241	couple	1236:1241	couple	1236:1241	Our data refine the complex interplay between an intimate couple of a lysosomal transporter and its accessory subunit.					
32959924	4	8	theme	N-glycans	718:726	arg1	type					710:713	the type	706:713	the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains	706:792	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	4	8	theme	N-glycans	718:726	arg1	essential					799:807	essential	799:807	essential	799:807	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	8	9	theme	lysosomal	1248:1256	arg1	transporter					1258:1268	a lysosomal transporter	1246:1268	a lysosomal transporter	1246:1268	Our data refine the complex interplay between an intimate couple of a lysosomal transporter and its accessory subunit.					
32959924	8	10	theme	subunit	1288:1294	arg1	intimate					1227:1234	intimate	1227:1234	intimate	1227:1234	Our data refine the complex interplay between an intimate couple of a lysosomal transporter and its accessory subunit.					
32959924	0	11	theme	lysosomal	39:47	arg1	transporter					58:68	the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP	35:105	the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP	35:105	Characterization of the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP.					
32959924	5	12	theme	proteins	866:873	arg1	interaction					846:856	the interaction	842:856	the interaction of both proteins	842:873	We observed that the interaction of both proteins already starts in the endoplasmic reticulum, and quantitatively depends on each other.					
32959924	2	13	theme	short	424:428	arg1	tail					440:443	its short cytosolic tail	420:443	its short cytosolic tail	420:443	We here refined the molecular interaction of the two proteins and found that the luminal domain of GLMP alone, but not its transmembrane domain or its short cytosolic tail, conveys protection and mediates the interaction with MFSD1.					
32959924	2	14	with	interaction	482:492	arg1	MFSD1					499:503	MFSD1	499:503	MFSD1	499:503	We here refined the molecular interaction of the two proteins and found that the luminal domain of GLMP alone, but not its transmembrane domain or its short cytosolic tail, conveys protection and mediates the interaction with MFSD1.					
32959924	5	15	theme	endoplasmic	897:907	arg1	reticulum					909:917	the endoplasmic reticulum	893:917	the endoplasmic reticulum	893:917	We observed that the interaction of both proteins already starts in the endoplasmic reticulum, and quantitatively depends on each other.					
32959924	2	16	theme	GLMP	372:375	arg1	GLMP					372:375	GLMP	372:375	GLMP alone	372:381	We here refined the molecular interaction of the two proteins and found that the luminal domain of GLMP alone, but not its transmembrane domain or its short cytosolic tail, conveys protection and mediates the interaction with MFSD1.					
32959924	2	16	theme	GLMP	372:375	arg1	domain					362:367	the luminal domain	350:367	the luminal domain of GLMP alone	350:381	We here refined the molecular interaction of the two proteins and found that the luminal domain of GLMP alone, but not its transmembrane domain or its short cytosolic tail, conveys protection and mediates the interaction with MFSD1.					
32959924	1	17	theme	tight	175:179	arg1	complex					181:187	a tight complex	173:187	a tight complex that confers protection of both interaction partners against lysosomal proteolysis	173:270	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	0	18	theme	complex	24:30	arg1	Characterization					0:15	Characterization	0:15	Characterization of the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP.	0:106	Characterization of the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP.					
32959924	8	19	theme	transporter	1258:1268	arg1	intimate					1227:1234	intimate	1227:1234	intimate	1227:1234	Our data refine the complex interplay between an intimate couple of a lysosomal transporter and its accessory subunit.					
32959924	6	20	from	trafficking	1018:1028	arg1	addition					1046:1053	addition	1046:1053	addition	1046:1053	Both proteins can affect vice versa their intracellular trafficking to lysosomes in addition to the protection from proteolysis.					
32959924	8	21	theme	accessory	1278:1286	arg1	subunit					1288:1294	its accessory subunit	1274:1294	its accessory subunit	1274:1294	Our data refine the complex interplay between an intimate couple of a lysosomal transporter and its accessory subunit.					
32959924	2	22	theme	transmembrane	396:408	arg1	domain					410:415	its transmembrane domain	392:415	its transmembrane domain	392:415	We here refined the molecular interaction of the two proteins and found that the luminal domain of GLMP alone, but not its transmembrane domain or its short cytosolic tail, conveys protection and mediates the interaction with MFSD1.					
32959924	2	23	theme	molecular	293:301	arg1	interaction					303:313	the molecular interaction	289:313	the molecular interaction of the two proteins	289:333	We here refined the molecular interaction of the two proteins and found that the luminal domain of GLMP alone, but not its transmembrane domain or its short cytosolic tail, conveys protection and mediates the interaction with MFSD1.					
32959924	4	24	theme	chains	787:792	arg1	type					710:713	the type	706:713	the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains	706:792	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	4	24	theme	chains	787:792	arg1	essential					799:807	essential	799:807	essential	799:807	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	8	25	theme	complex	1198:1204	arg1	interplay					1206:1214	the complex interplay	1194:1214	the complex interplay between an intimate couple of a lysosomal transporter and its accessory subunit	1194:1294	Our data refine the complex interplay between an intimate couple of a lysosomal transporter and its accessory subunit.					
32959924	6	26	theme	intracellular	1004:1016	arg1	trafficking					1018:1028	their intracellular trafficking	998:1028	their intracellular trafficking to lysosomes in addition to the protection from proteolysis	998:1088	Both proteins can affect vice versa their intracellular trafficking to lysosomes in addition to the protection from proteolysis.					
32959924	4	27	theme	N-glycan	778:785	arg1	chains					787:792	individual N-glycan chains	767:792	individual N-glycan chains	767:792	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	0	28	theme	membrane	49:56	arg1	transporter					58:68	the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP	35:105	the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP	35:105	Characterization of the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP.					
32959924	2	29	theme	cytosolic	430:438	arg1	tail					440:443	its short cytosolic tail	420:443	its short cytosolic tail	420:443	We here refined the molecular interaction of the two proteins and found that the luminal domain of GLMP alone, but not its transmembrane domain or its short cytosolic tail, conveys protection and mediates the interaction with MFSD1.					
32959924	4	30	gly	N-glycosylation	662:676	arg1	general					689:695	general	689:695	general	689:695	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	4	30	gly	N-glycosylation	662:676	arg1	GLMP					681:684	GLMP	681:684	GLMP	681:684	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	1	31	dep	proteins	144:151	arg1	proteins					144:151	The two lysosomal integral membrane proteins	108:151	The two lysosomal integral membrane proteins MFSD1 and GLMP	108:166	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	1	31	dep	proteins	144:151	arg1	GLMP					163:166	GLMP	163:166	GLMP	163:166	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	1	31	dep	proteins	144:151	arg1	MFSD1					153:157	MFSD1	153:157	MFSD1	153:157	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	1	32	theme	lysosomal	116:124	arg1	proteins					144:151	The two lysosomal integral membrane proteins	108:151	The two lysosomal integral membrane proteins MFSD1 and GLMP	108:166	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	1	32	theme	lysosomal	116:124	arg1	GLMP					163:166	GLMP	163:166	GLMP	163:166	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	1	32	theme	lysosomal	116:124	arg1	MFSD1					153:157	MFSD1	153:157	MFSD1	153:157	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	3	33	theme	complex	598:604	arg1	stabilization					577:589	the stabilization	573:589	the stabilization of the complex	573:604	Our data support the finding that the interaction is essential for the stabilization of the complex.					
32959924	1	34	theme	integral	126:133	arg1	proteins					144:151	The two lysosomal integral membrane proteins	108:151	The two lysosomal integral membrane proteins MFSD1 and GLMP	108:166	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	1	34	theme	integral	126:133	arg1	GLMP					163:166	GLMP	163:166	GLMP	163:166	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	1	34	theme	integral	126:133	arg1	MFSD1					153:157	MFSD1	153:157	MFSD1	153:157	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	2	35	theme	proteins	326:333	arg1	interaction					303:313	the molecular interaction	289:313	the molecular interaction of the two proteins	289:333	We here refined the molecular interaction of the two proteins and found that the luminal domain of GLMP alone, but not its transmembrane domain or its short cytosolic tail, conveys protection and mediates the interaction with MFSD1.					
32959924	4	36	theme	individual	767:776	arg1	chains					787:792	individual N-glycan chains	767:792	individual N-glycan chains	767:792	These results are complemented by the observation that N-glycosylation of GLMP in general, but not the type of N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, are essential for protection.					
32959924	1	37	theme	membrane	135:142	arg1	proteins					144:151	The two lysosomal integral membrane proteins	108:151	The two lysosomal integral membrane proteins MFSD1 and GLMP	108:166	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	1	37	theme	membrane	135:142	arg1	GLMP					163:166	GLMP	163:166	GLMP	163:166	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	1	37	theme	membrane	135:142	arg1	MFSD1					153:157	MFSD1	153:157	MFSD1	153:157	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	1	38	theme	interaction	221:231	arg1	partners					233:240	both interaction partners	216:240	both interaction partners	216:240	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
32959924	0	39	theme	transporter	58:68	arg1	complex					24:30	the complex	20:30	the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP	20:105	Characterization of the complex of the lysosomal membrane transporter MFSD1 and its accessory subunit GLMP.					
32959924	6	40	from	proteolysis	1078:1088	arg1	protection					1062:1071	the protection	1058:1071	the protection from proteolysis	1058:1088	Both proteins can affect vice versa their intracellular trafficking to lysosomes in addition to the protection from proteolysis.					
32959924	2	41	theme	luminal	354:360	arg1	GLMP					372:375	GLMP	372:375	GLMP alone	372:381	We here refined the molecular interaction of the two proteins and found that the luminal domain of GLMP alone, but not its transmembrane domain or its short cytosolic tail, conveys protection and mediates the interaction with MFSD1.					
32959924	2	41	theme	luminal	354:360	arg1	domain					362:367	the luminal domain	350:367	the luminal domain of GLMP alone	350:381	We here refined the molecular interaction of the two proteins and found that the luminal domain of GLMP alone, but not its transmembrane domain or its short cytosolic tail, conveys protection and mediates the interaction with MFSD1.					
32959924	1	42	theme	partners	233:240	arg1	protection					202:211	protection	202:211	protection of both interaction partners against lysosomal proteolysis	202:270	The two lysosomal integral membrane proteins MFSD1 and GLMP form a tight complex that confers protection of both interaction partners against lysosomal proteolysis.					
33788319	0	0	theme	plant	117:121	arg1	polysaccharides					140:154	plant cell wall matrix polysaccharides	117:154	plant cell wall matrix polysaccharides	117:154	Gel-permeation chromatography-enzyme-linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides.					
33788319	8	1	theme	wall	1605:1608	arg1	composition					1610:1620	wall composition	1605:1620	wall composition	1605:1620	As demonstrated in this article, this methodology allows a much deeper understanding of wall composition, its dynamism and plasticity to be obtained, furthering our knowledge of cell wall biology.					
33788319	1	2	theme	multi-component	184:198	arg1	walls					162:166	Cell walls	157:166	Cell walls	157:166	Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology.					
33788319	1	2	theme	multi-component	184:198	arg1	materials					200:208	dynamic and multi-component materials	172:208	dynamic and multi-component materials that play important roles in many areas of plant biology	172:265	Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology.					
33788319	5	3	theme	polymers	1017:1024	arg1	fractionation					990:1002	fractionation	990:1002	fractionation of cell wall polymers followed by enzyme-linked immunosorbent assay	990:1070	The method employs gel-permeation chromatography fractionation of cell wall polymers followed by enzyme-linked immunosorbent assay to identify polymer types.					
33788319	4	4	theme	indirect	891:898	arg1	consequences					900:911	direct and indirect consequences	880:911	direct and indirect consequences of cell wall manipulations	880:938	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	5	5	link	enzyme-linked	1038:1050	arg1	assay					1066:1070	enzyme-linked immunosorbent assay	1038:1070	enzyme-linked immunosorbent assay	1038:1070	The method employs gel-permeation chromatography fractionation of cell wall polymers followed by enzyme-linked immunosorbent assay to identify polymer types.					
33788319	3	6	theme	enzymes	448:454	arg1	Studies					423:429	Studies	423:429	Studies of the genes and enzymes that determine wall composition and function	423:499	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	5	7	theme	immunosorbent	1052:1064	arg1	assay					1066:1070	enzyme-linked immunosorbent assay	1038:1070	enzyme-linked immunosorbent assay	1038:1070	The method employs gel-permeation chromatography fractionation of cell wall polymers followed by enzyme-linked immunosorbent assay to identify polymer types.					
33788319	0	8	theme	wall	128:131	arg1	polysaccharides					140:154	plant cell wall matrix polysaccharides	117:154	plant cell wall matrix polysaccharides	117:154	Gel-permeation chromatography-enzyme-linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides.					
33788319	4	9	theme	wall	921:924	arg1	manipulations					926:938	cell wall manipulations	916:938	cell wall manipulations	916:938	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	7	10	from	diversity	1360:1368	arg1	organs					1395:1400	different Arabidopsis organs	1373:1400	different Arabidopsis organs	1373:1400	The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants.					
33788319	3	11	theme	compensatory	564:575	arg1	changes					577:583	potential compensatory changes	554:583	potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides	554:706	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	3	12	from	changes	577:583	arg1	polymers					593:600	wall polymers	588:600	wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides	588:706	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	0	13	theme	cell	123:126	arg1	polysaccharides					140:154	plant cell wall matrix polysaccharides	117:154	plant cell wall matrix polysaccharides	117:154	Gel-permeation chromatography-enzyme-linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides.					
33788319	3	14	theme	changes	577:583	arg1	account					543:549	account	543:549	account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides	543:706	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	7	15	theme	compositional	1346:1358	arg1	diversity					1360:1368	compositional diversity	1346:1368	compositional diversity in different Arabidopsis organs	1346:1400	The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants.					
33788319	2	16	theme	elements	319:326	arg1	interactions					288:299	interactions	288:299	interactions	288:299	The composition and interactions of the structural elements give rise to material properties, which are modulated by the activity of wall-related enzymes.					
33788319	2	16	theme	elements	319:326	arg1	composition					272:282	composition	272:282	composition	272:282	The composition and interactions of the structural elements give rise to material properties, which are modulated by the activity of wall-related enzymes.					
33788319	3	17	from	changes	637:643	arg1	components					654:663	other components	648:663	other components	648:663	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	7	18	theme	Arabidopsis	1383:1393	arg1	organs					1395:1400	different Arabidopsis organs	1373:1400	different Arabidopsis organs	1373:1400	The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants.					
33788319	0	19	theme	polysaccharides	140:154	arg1	profiling					104:112	systematic mass distribution profiling	75:112	systematic mass distribution profiling of plant cell wall matrix polysaccharides	75:154	Gel-permeation chromatography-enzyme-linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides.					
33788319	8	20	theme	dynamism	1627:1634	arg1	understanding					1588:1600	a much deeper understanding	1574:1600	a much deeper understanding of wall composition, its dynamism and plasticity	1574:1649	As demonstrated in this article, this methodology allows a much deeper understanding of wall composition, its dynamism and plasticity to be obtained, furthering our knowledge of cell wall biology.					
33788319	4	21	theme	direct	880:885	arg1	consequences					900:911	direct and indirect consequences	880:911	direct and indirect consequences of cell wall manipulations	880:938	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	3	22	from	account	543:549	arg1	polymers					593:600	wall polymers	588:600	wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides	588:706	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	2	23	theme	structural	308:317	arg1	elements					319:326	the structural elements	304:326	the structural elements	304:326	The composition and interactions of the structural elements give rise to material properties, which are modulated by the activity of wall-related enzymes.					
33788319	1	24	theme	important	220:228	arg1	roles					230:234	important roles	220:234	important roles	220:234	Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology.					
33788319	6	25	theme	monoclonal	1165:1174	arg1	antibodies					1176:1185	glycan-directed monoclonal antibodies	1149:1185	glycan-directed monoclonal antibodies	1149:1185	We demonstrate the potential of this method using glycan-directed monoclonal antibodies to detect epitopes representing xyloglucans, heteromannans, glucuronoxylans, homogalacturonans (HGs) and methyl-esterified HGs.					
33788319	0	26	theme	matrix	133:138	arg1	polysaccharides					140:154	plant cell wall matrix polysaccharides	117:154	plant cell wall matrix polysaccharides	117:154	Gel-permeation chromatography-enzyme-linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides.					
33788319	3	27	theme	potential	554:562	arg1	changes					577:583	potential compensatory changes	554:583	potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides	554:706	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	2	28	dep	composition	272:282	arg1	The					268:270	The	268:270	The	268:270	The composition and interactions of the structural elements give rise to material properties, which are modulated by the activity of wall-related enzymes.					
33788319	8	29	theme	plasticity	1640:1649	arg1	understanding					1588:1600	a much deeper understanding	1574:1600	a much deeper understanding of wall composition, its dynamism and plasticity	1574:1649	As demonstrated in this article, this methodology allows a much deeper understanding of wall composition, its dynamism and plasticity to be obtained, furthering our knowledge of cell wall biology.					
33788319	3	30	dep	genes	438:442	arg1	the					434:436	the	434:436	the	434:436	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	2	31	theme	enzymes	414:420	arg1	activity					389:396	the activity	385:396	the activity of wall-related enzymes	385:420	The composition and interactions of the structural elements give rise to material properties, which are modulated by the activity of wall-related enzymes.					
33788319	2	32	theme	material	341:348	arg1	properties					350:359	material properties	341:359	material properties	341:359	The composition and interactions of the structural elements give rise to material properties, which are modulated by the activity of wall-related enzymes.					
33788319	4	33	theme	cell	916:919	arg1	manipulations					926:938	cell wall manipulations	916:938	cell wall manipulations	916:938	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	2	34	theme	wall-related	401:412	arg1	enzymes					414:420	wall-related enzymes	401:420	wall-related enzymes	401:420	The composition and interactions of the structural elements give rise to material properties, which are modulated by the activity of wall-related enzymes.					
33788319	0	35	theme	assay	58:62	arg1	method					64:69	immunosorbent assay method	44:69	immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides	44:154	Gel-permeation chromatography-enzyme-linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides.					
33788319	6	36	theme	glycan-directed	1149:1163	arg1	antibodies					1176:1185	glycan-directed monoclonal antibodies	1149:1185	glycan-directed monoclonal antibodies	1149:1185	We demonstrate the potential of this method using glycan-directed monoclonal antibodies to detect epitopes representing xyloglucans, heteromannans, glucuronoxylans, homogalacturonans (HGs) and methyl-esterified HGs.					
33788319	4	37	theme	mass	783:786	arg1	distributions					788:800	the mass distributions	779:800	the mass distributions	779:800	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	0	38	theme	immunosorbent	44:56	arg1	method					64:69	immunosorbent assay method	44:69	immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides	44:154	Gel-permeation chromatography-enzyme-linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides.					
33788319	5	39	theme	cell	1007:1010	arg1	polymers					1017:1024	cell wall polymers	1007:1024	cell wall polymers	1007:1024	The method employs gel-permeation chromatography fractionation of cell wall polymers followed by enzyme-linked immunosorbent assay to identify polymer types.					
33788319	6	40	theme	methyl-esterified	1292:1308	arg1	HGs					1310:1312	methyl-esterified HGs	1292:1312	methyl-esterified HGs	1292:1312	We demonstrate the potential of this method using glycan-directed monoclonal antibodies to detect epitopes representing xyloglucans, heteromannans, glucuronoxylans, homogalacturonans (HGs) and methyl-esterified HGs.					
33788319	3	41	theme	wall	588:591	arg1	polymers					593:600	wall polymers	588:600	wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides	588:706	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	1	42	theme	many	239:242	arg1	areas					244:248	many areas	239:248	many areas of plant biology	239:265	Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology.					
33788319	3	43	theme	genes	438:442	arg1	Studies					423:429	Studies	423:429	Studies of the genes and enzymes that determine wall composition and function	423:499	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	5	44	theme	gel-permeation	960:973	arg1	chromatography					975:988	gel-permeation chromatography	960:988	gel-permeation chromatography	960:988	The method employs gel-permeation chromatography fractionation of cell wall polymers followed by enzyme-linked immunosorbent assay to identify polymer types.					
33788319	3	45	theme	specific	683:690	arg1	polysaccharides					692:706	specific polysaccharides	683:706	specific polysaccharides	683:706	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	7	46	theme	cell	1498:1501	arg1	mutants					1508:1514	previously characterized cell wall mutants	1473:1514	previously characterized cell wall mutants	1473:1514	The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants.					
33788319	6	47	theme	method	1136:1141	arg1	potential					1118:1126	the potential	1114:1126	the potential of this method using glycan-directed monoclonal antibodies to detect epitopes representing xyloglucans, heteromannans, glucuronoxylans, homogalacturonans (HGs) and methyl-esterified HGs	1114:1312	We demonstrate the potential of this method using glycan-directed monoclonal antibodies to detect epitopes representing xyloglucans, heteromannans, glucuronoxylans, homogalacturonans (HGs) and methyl-esterified HGs.					
33788319	7	48	theme	wall	1441:1444	arg1	composition					1446:1456	wall composition	1441:1456	wall composition	1441:1456	The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants.					
33788319	7	49	theme	mutants	1508:1514	arg1	number					1463:1468	a number	1461:1468	a number of previously characterized cell wall mutants	1461:1514	The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants.					
33788319	4	50	theme	distributions	788:800	arg1	examination					764:774	the simultaneous examination	747:774	the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components	747:855	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	4	51	theme	components	846:855	arg1	quantities					806:815	quantities	806:815	quantities	806:815	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	4	51	theme	components	846:855	arg1	distributions					788:800	the mass distributions	779:800	the mass distributions	779:800	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	0	52	theme	mass	86:89	arg1	profiling					104:112	systematic mass distribution profiling	75:112	systematic mass distribution profiling of plant cell wall matrix polysaccharides	75:154	Gel-permeation chromatography-enzyme-linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides.					
33788319	3	53	dep	accompany	611:619	arg1	may					607:609	may	607:609	may	607:609	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	8	54	theme	wall	1700:1703	arg1	biology					1705:1711	cell wall biology	1695:1711	cell wall biology	1695:1711	As demonstrated in this article, this methodology allows a much deeper understanding of wall composition, its dynamism and plasticity to be obtained, furthering our knowledge of cell wall biology.					
33788319	0	55	theme	systematic	75:84	arg1	profiling					104:112	systematic mass distribution profiling	75:112	systematic mass distribution profiling of plant cell wall matrix polysaccharides	75:154	Gel-permeation chromatography-enzyme-linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides.					
33788319	8	56	theme	cell	1695:1698	arg1	biology					1705:1711	cell wall biology	1695:1711	cell wall biology	1695:1711	As demonstrated in this article, this methodology allows a much deeper understanding of wall composition, its dynamism and plasticity to be obtained, furthering our knowledge of cell wall biology.					
33788319	4	57	theme	manipulations	926:938	arg1	consequences					900:911	direct and indirect consequences	880:911	direct and indirect consequences of cell wall manipulations	880:938	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	8	58	theme	deeper	1581:1586	arg1	understanding					1588:1600	a much deeper understanding	1574:1600	a much deeper understanding of wall composition, its dynamism and plasticity	1574:1649	As demonstrated in this article, this methodology allows a much deeper understanding of wall composition, its dynamism and plasticity to be obtained, furthering our knowledge of cell wall biology.					
33788319	7	59	theme	characterized	1484:1496	arg1	mutants					1508:1514	previously characterized cell wall mutants	1473:1514	previously characterized cell wall mutants	1473:1514	The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants.					
33788319	1	60	theme	Cell	157:160	arg1	walls					162:166	Cell walls	157:166	Cell walls	157:166	Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology.					
33788319	1	60	theme	Cell	157:160	arg1	materials					200:208	dynamic and multi-component materials	172:208	dynamic and multi-component materials that play important roles in many areas of plant biology	172:265	Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology.					
33788319	5	61	theme	polymer	1084:1090	arg1	types					1092:1096	polymer types	1084:1096	polymer types	1084:1096	The method employs gel-permeation chromatography fractionation of cell wall polymers followed by enzyme-linked immunosorbent assay to identify polymer types.					
33788319	4	62	theme	cell	829:832	arg1	components					846:855	specific cell wall matrix components	820:855	specific cell wall matrix components	820:855	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	3	63	theme	other	648:652	arg1	components					654:663	other components	648:663	other components	648:663	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	7	64	theme	wall	1503:1506	arg1	mutants					1508:1514	previously characterized cell wall mutants	1473:1514	previously characterized cell wall mutants	1473:1514	The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants.					
33788319	4	65	theme	simultaneous	751:762	arg1	examination					764:774	the simultaneous examination	747:774	the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components	747:855	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	5	66	theme	wall	1012:1015	arg1	polymers					1017:1024	cell wall polymers	1007:1024	cell wall polymers	1007:1024	The method employs gel-permeation chromatography fractionation of cell wall polymers followed by enzyme-linked immunosorbent assay to identify polymer types.					
33788319	1	67	theme	plant	253:257	arg1	biology					259:265	plant biology	253:265	plant biology	253:265	Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology.					
33788319	4	68	theme	quantities	806:815	arg1	examination					764:774	the simultaneous examination	747:774	the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components	747:855	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	7	69	used	used	1330:1333	arg2	method					1319:1324	The method	1315:1324	The method	1315:1324	The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants.					
33788319	4	70	theme	specific	820:827	arg1	components					846:855	specific cell wall matrix components	820:855	specific cell wall matrix components	820:855	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	3	71	theme	great	511:515	arg1	progress					517:524	great progress	511:524	great progress	511:524	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	3	72	from	polymers	593:600	arg1	account					543:549	account	543:549	account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides	543:706	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	1	73	theme	biology	259:265	arg1	areas					244:248	many areas	239:248	many areas of plant biology	239:265	Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology.					
33788319	5	74	theme	enzyme-linked	1038:1050	arg1	assay					1066:1070	enzyme-linked immunosorbent assay	1038:1070	enzyme-linked immunosorbent assay	1038:1070	The method employs gel-permeation chromatography fractionation of cell wall polymers followed by enzyme-linked immunosorbent assay to identify polymer types.					
33788319	4	75	theme	matrix	839:844	arg1	components					846:855	specific cell wall matrix components	820:855	specific cell wall matrix components	820:855	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	1	76	theme	dynamic	172:178	arg1	walls					162:166	Cell walls	157:166	Cell walls	157:166	Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology.					
33788319	1	76	theme	dynamic	172:178	arg1	materials					200:208	dynamic and multi-component materials	172:208	dynamic and multi-component materials that play important roles in many areas of plant biology	172:265	Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology.					
33788319	3	77	theme	wall	471:474	arg1	composition					476:486	wall composition	471:486	wall composition	471:486	Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides.					
33788319	0	78	theme	distribution	91:102	arg1	profiling					104:112	systematic mass distribution profiling	75:112	systematic mass distribution profiling of plant cell wall matrix polysaccharides	75:154	Gel-permeation chromatography-enzyme-linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides.					
33788319	8	79	theme	composition	1610:1620	arg1	understanding					1588:1600	a much deeper understanding	1574:1600	a much deeper understanding of wall composition, its dynamism and plasticity	1574:1649	As demonstrated in this article, this methodology allows a much deeper understanding of wall composition, its dynamism and plasticity to be obtained, furthering our knowledge of cell wall biology.					
33788319	4	80	theme	wall	834:837	arg1	components					846:855	specific cell wall matrix components	820:855	specific cell wall matrix components	820:855	Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations.					
33788319	7	81	theme	different	1373:1381	arg1	organs					1395:1400	different Arabidopsis organs	1373:1400	different Arabidopsis organs	1373:1400	The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants.					
33788319	8	82	theme	biology	1705:1711	arg1	knowledge					1682:1690	our knowledge	1678:1690	our knowledge of cell wall biology	1678:1711	As demonstrated in this article, this methodology allows a much deeper understanding of wall composition, its dynamism and plasticity to be obtained, furthering our knowledge of cell wall biology.					
32593796	0	0	theme	Maillard	91:98	arg1	reaction					100:107	Maillard reaction	91:107	Maillard reaction	91:107	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	0	1	theme	emulsions	193:201	arg1	heating					151:157	slit divergent ultrasonic assisted wet heating	112:157	slit divergent ultrasonic assisted wet heating	112:157	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	0	1	theme	emulsions	193:201	arg1	stability					167:175	the stability	163:175	the stability of oil-in-water emulsions	163:201	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	0	2	theme	oil-in-water	180:191	arg1	emulsions					193:201	oil-in-water emulsions	180:201	oil-in-water emulsions	180:201	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	1	3	theme	treatment	255:263	arg1	Effects					204:210	Effects	204:210	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction	204:338	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	0	4	theme	divergent	117:125	arg1	heating					151:157	slit divergent ultrasonic assisted wet heating	112:157	slit divergent ultrasonic assisted wet heating	112:157	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	5	5	theme	conjugates	1074:1083	arg1	capacity					1027:1034	emulsifying capacity	1015:1034	emulsifying capacity	1015:1034	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	5	theme	conjugates	1074:1083	arg1	solubility					994:1003	solubility	994:1003	solubility	994:1003	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	5	theme	conjugates	1074:1083	arg1	stability					1045:1053	thermal stability	1037:1053	thermal stability	1037:1053	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	5	theme	conjugates	1074:1083	arg1	foaming					1006:1012	foaming	1006:1012	foaming	1006:1012	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	5	theme	conjugates	1074:1083	arg1	viscosity					1060:1068	viscosity	1060:1068	viscosity	1060:1068	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	5	theme	conjugates	1074:1083	arg1	properties					982:991	the functional properties	967:991	the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates	967:1083	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	4	6	theme	surface	807:813	arg1	hydrophobicity					815:828	higher surface hydrophobicity	800:828	higher surface hydrophobicity	800:828	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	7	7	theme	functional	1326:1335	arg1	properties					1337:1346	the functional properties	1322:1346	the functional properties of conjugates	1322:1360	Taken together, SDU-assisted heating could be an excellent method to improve the functional properties of conjugates.					
32593796	0	8	theme	slit	112:115	arg1	heating					151:157	slit divergent ultrasonic assisted wet heating	112:157	slit divergent ultrasonic assisted wet heating	112:157	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	2	9	dep	conjugates	427:436	arg1	ultrasound					439:448	ultrasound	439:448	ultrasound	439:448	Besides, the stability of emulsions prepared by SPI and SPI-lentinan conjugates (ultrasound and un-ultrasound) as emulsifiers was compared.					
32593796	2	9	dep	conjugates	427:436	arg1	un-ultrasound					454:466	un-ultrasound	454:466	un-ultrasound	454:466	Besides, the stability of emulsions prepared by SPI and SPI-lentinan conjugates (ultrasound and un-ultrasound) as emulsifiers was compared.					
32593796	2	10	theme	emulsions	384:392	arg1	stability					371:379	the stability	367:379	the stability of emulsions prepared by SPI and SPI-lentinan conjugates (ultrasound and un-ultrasound) as emulsifiers	367:482	Besides, the stability of emulsions prepared by SPI and SPI-lentinan conjugates (ultrasound and un-ultrasound) as emulsifiers was compared.					
32593796	4	11	theme	structural	687:696	arg1	analysis					698:705	structural analysis	687:705	structural analysis	687:705	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	3	12	theme	traditional	632:642	arg1	method					652:657	traditional heating method	632:657	traditional heating method (2 h, 13.89%)	632:671	The results showed that ultrasonic treatment (40 min) markedly increased the degree of grafting (26.48%) by 1.91 times comparing with traditional heating method (2 h, 13.89%).					
32593796	4	13	contain	had	796:798	arg1	conjugates					723:732	the conjugates	719:732	the conjugates obtained by SDU treatment	719:758	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	4	13	contain	had	796:798	arg2	intensity					847:855	fluorescence intensity	834:855	fluorescence intensity	834:855	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	4	13	contain	had	796:798	arg2	hydrophobicity					815:828	higher surface hydrophobicity	800:828	higher surface hydrophobicity	800:828	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	0	14	theme	assisted	138:145	arg1	heating					151:157	slit divergent ultrasonic assisted wet heating	112:157	slit divergent ultrasonic assisted wet heating	112:157	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	5	15	theme	emulsifying	1015:1025	arg1	capacity					1027:1034	emulsifying capacity	1015:1034	emulsifying capacity	1015:1034	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	15	theme	emulsifying	1015:1025	arg1	properties					982:991	the functional properties	967:991	the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates	967:1083	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	4	16	theme	fluorescence	834:845	arg1	intensity					847:855	fluorescence intensity	834:855	fluorescence intensity	834:855	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	2	17	theme	SPI-lentinan	414:425	arg1	conjugates					427:436	SPI and SPI-lentinan conjugates	406:436	conjugates	427:436	Besides, the stability of emulsions prepared by SPI and SPI-lentinan conjugates (ultrasound and un-ultrasound) as emulsifiers was compared.					
32593796	0	18	theme	functional	14:23	arg1	properties					25:34	functional properties	14:34	functional properties	14:34	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	3	19	dep	method	652:657	arg1	%					670:670	13.89%	665:670	13.89%	665:670	The results showed that ultrasonic treatment (40 min) markedly increased the degree of grafting (26.48%) by 1.91 times comparing with traditional heating method (2 h, 13.89%).					
32593796	3	19	dep	method	652:657	arg1	2 h					660:662	2 h	660:662	2 h	660:662	The results showed that ultrasonic treatment (40 min) markedly increased the degree of grafting (26.48%) by 1.91 times comparing with traditional heating method (2 h, 13.89%).					
32593796	0	20	theme	ultrasonic	127:136	arg1	heating					151:157	slit divergent ultrasonic assisted wet heating	112:157	slit divergent ultrasonic assisted wet heating	112:157	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	0	21	dep	conjugates	68:77	arg1	obtained					79:86	obtained	79:86	conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions	68:201	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	6	22	theme	SPI-lentinan	1129:1140	arg1	ultrasound					1154:1163	ultrasound	1154:1163	ultrasound	1154:1163	Furthermore, the emulsions prepared by the SPI-lentinan conjugates (ultrasound) as emulsifiers possessed the highest stability against environmental stresses.					
32593796	6	22	theme	SPI-lentinan	1129:1140	arg1	conjugates					1142:1151	the SPI-lentinan conjugates	1125:1151	the SPI-lentinan conjugates (ultrasound) as emulsifiers	1125:1179	Furthermore, the emulsions prepared by the SPI-lentinan conjugates (ultrasound) as emulsifiers possessed the highest stability against environmental stresses.					
32593796	0	23	theme	soy	39:41	arg1	isolate-lentinan					51:66	soy protein isolate-lentinan	39:66	soy protein isolate-lentinan	39:66	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	7	24	theme	excellent	1294:1302	arg1	method					1304:1309	an excellent method	1291:1309	an excellent method to improve the functional properties of conjugates	1291:1360	Taken together, SDU-assisted heating could be an excellent method to improve the functional properties of conjugates.					
32593796	7	24	theme	excellent	1294:1302	arg1	heating					1274:1280	SDU-assisted heating	1261:1280	SDU-assisted heating	1261:1280	Taken together, SDU-assisted heating could be an excellent method to improve the functional properties of conjugates.					
32593796	1	25	theme	novel	217:221	arg1	SDU					250:252	SDU	250:252	SDU	250:252	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	1	25	theme	novel	217:221	arg1	ultrasound					238:247	a novel slit divergent ultrasound	215:247	a novel slit divergent ultrasound (SDU) treatment	215:263	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	2	26	theme	SPI	406:408	arg1	conjugates					427:436	SPI and SPI-lentinan conjugates	406:436	conjugates	427:436	Besides, the stability of emulsions prepared by SPI and SPI-lentinan conjugates (ultrasound and un-ultrasound) as emulsifiers was compared.					
32593796	0	27	theme	wet	147:149	arg1	heating					151:157	slit divergent ultrasonic assisted wet heating	112:157	slit divergent ultrasonic assisted wet heating	112:157	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	4	28	theme	secondary	772:780	arg1	structure					782:790	the secondary structure	768:790	the secondary structure	768:790	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	1	29	theme	slit	223:226	arg1	SDU					250:252	SDU	250:252	SDU	250:252	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	1	29	theme	slit	223:226	arg1	ultrasound					238:247	a novel slit divergent ultrasound	215:247	a novel slit divergent ultrasound (SDU) treatment	215:263	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	0	30	theme	isolate-lentinan	51:66	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	0	30	theme	isolate-lentinan	51:66	arg1	properties					25:34	functional properties	14:34	functional properties	14:34	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	1	31	theme	-lentinan	297:305	arg1	conjugates					307:316	soybean protein isolate (SPI)-lentinan conjugates	268:316	soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction	268:338	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	0	32	theme	protein	43:49	arg1	isolate-lentinan					51:66	soy protein isolate-lentinan	39:66	soy protein isolate-lentinan	39:66	Structure and functional properties of soy protein isolate-lentinan conjugates obtained in Maillard reaction by slit divergent ultrasonic assisted wet heating and the stability of oil-in-water emulsions.					
32593796	4	33	theme	higher	800:805	arg1	hydrophobicity					815:828	higher surface hydrophobicity	800:828	higher surface hydrophobicity	800:828	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	3	34	theme	grafting	585:592	arg1	degree					575:580	the degree	571:580	the degree of grafting (26.48%)	571:601	The results showed that ultrasonic treatment (40 min) markedly increased the degree of grafting (26.48%) by 1.91 times comparing with traditional heating method (2 h, 13.89%).					
32593796	4	35	theme	heating	892:898	arg1	method					900:905	traditional heating method	880:905	traditional heating method	880:905	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	6	36	theme	highest	1195:1201	arg1	stability					1203:1211	the highest stability	1191:1211	the highest stability against environmental stresses	1191:1242	Furthermore, the emulsions prepared by the SPI-lentinan conjugates (ultrasound) as emulsifiers possessed the highest stability against environmental stresses.					
32593796	5	37	theme	functional	971:980	arg1	capacity					1027:1034	emulsifying capacity	1015:1034	emulsifying capacity	1015:1034	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	37	theme	functional	971:980	arg1	solubility					994:1003	solubility	994:1003	solubility	994:1003	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	37	theme	functional	971:980	arg1	stability					1045:1053	thermal stability	1037:1053	thermal stability	1037:1053	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	37	theme	functional	971:980	arg1	foaming					1006:1012	foaming	1006:1012	foaming	1006:1012	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	37	theme	functional	971:980	arg1	viscosity					1060:1068	viscosity	1060:1068	viscosity	1060:1068	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	37	theme	functional	971:980	arg1	properties					982:991	the functional properties	967:991	the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates	967:1083	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	6	38	theme	environmental	1221:1233	arg1	stresses					1235:1242	environmental stresses	1221:1242	environmental stresses	1221:1242	Furthermore, the emulsions prepared by the SPI-lentinan conjugates (ultrasound) as emulsifiers possessed the highest stability against environmental stresses.					
32593796	7	39	theme	SDU-assisted	1261:1272	arg1	method					1304:1309	an excellent method	1291:1309	an excellent method to improve the functional properties of conjugates	1291:1360	Taken together, SDU-assisted heating could be an excellent method to improve the functional properties of conjugates.					
32593796	7	39	theme	SDU-assisted	1261:1272	arg1	heating					1274:1280	SDU-assisted heating	1261:1280	SDU-assisted heating	1261:1280	Taken together, SDU-assisted heating could be an excellent method to improve the functional properties of conjugates.					
32593796	5	40	theme	SDU	925:927	arg1	treatment					929:937	SDU treatment	925:937	SDU treatment	925:937	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	1	41	theme	divergent	228:236	arg1	SDU					250:252	SDU	250:252	SDU	250:252	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	1	41	theme	divergent	228:236	arg1	ultrasound					238:247	a novel slit divergent ultrasound	215:247	a novel slit divergent ultrasound (SDU) treatment	215:263	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	4	42	theme	traditional	880:890	arg1	method					900:905	traditional heating method	880:905	traditional heating method	880:905	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	3	43	theme	heating	644:650	arg1	method					652:657	traditional heating method	632:657	traditional heating method (2 h, 13.89%)	632:671	The results showed that ultrasonic treatment (40 min) markedly increased the degree of grafting (26.48%) by 1.91 times comparing with traditional heating method (2 h, 13.89%).					
32593796	4	44	theme	SDU	746:748	arg1	treatment					750:758	SDU treatment	746:758	SDU treatment	746:758	In addition, structural analysis showed that the conjugates obtained by SDU treatment changed the secondary structure and had higher surface hydrophobicity and fluorescence intensity than those obtained by traditional heating method.					
32593796	5	45	theme	thermal	1037:1043	arg1	stability					1045:1053	thermal stability	1037:1053	thermal stability	1037:1053	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	45	theme	thermal	1037:1043	arg1	properties					982:991	the functional properties	967:991	the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates	967:1083	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	1	46	theme	ultrasound	238:247	arg1	treatment					255:263	a novel slit divergent ultrasound (SDU) treatment	215:263	a novel slit divergent ultrasound (SDU) treatment	215:263	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	5	47	dep	properties	982:991	arg1	capacity					1027:1034	emulsifying capacity	1015:1034	emulsifying capacity	1015:1034	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	47	dep	properties	982:991	arg1	solubility					994:1003	solubility	994:1003	solubility	994:1003	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	47	dep	properties	982:991	arg1	stability					1045:1053	thermal stability	1037:1053	thermal stability	1037:1053	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	47	dep	properties	982:991	arg1	foaming					1006:1012	foaming	1006:1012	foaming	1006:1012	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	47	dep	properties	982:991	arg1	viscosity					1060:1068	viscosity	1060:1068	viscosity	1060:1068	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	5	47	dep	properties	982:991	arg1	properties					982:991	the functional properties	967:991	the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates	967:1083	Apart from this, SDU treatment could significantly improve the functional properties (solubility, foaming, emulsifying capacity, thermal stability, and viscosity) of conjugates.					
32593796	1	48	from	Effects	204:210	arg1	conjugates					307:316	soybean protein isolate (SPI)-lentinan conjugates	268:316	soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction	268:338	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	1	49	theme	Maillard	322:329	arg1	reaction					331:338	Maillard reaction	322:338	Maillard reaction	322:338	Effects of a novel slit divergent ultrasound (SDU) treatment on soybean protein isolate (SPI)-lentinan conjugates via Maillard reaction was investigated.					
32593796	7	50	theme	conjugates	1351:1360	arg1	properties					1337:1346	the functional properties	1322:1346	the functional properties of conjugates	1322:1360	Taken together, SDU-assisted heating could be an excellent method to improve the functional properties of conjugates.					
32593796	3	51	theme	ultrasonic	522:531	arg1	40 min					544:549	40 min	544:549	40 min	544:549	The results showed that ultrasonic treatment (40 min) markedly increased the degree of grafting (26.48%) by 1.91 times comparing with traditional heating method (2 h, 13.89%).					
32593796	3	51	theme	ultrasonic	522:531	arg1	treatment					533:541	ultrasonic treatment	522:541	ultrasonic treatment (40 min)	522:550	The results showed that ultrasonic treatment (40 min) markedly increased the degree of grafting (26.48%) by 1.91 times comparing with traditional heating method (2 h, 13.89%).					
32593796	6	52	contain	possessed	1181:1189	arg1	emulsions					1103:1111	the emulsions	1099:1111	the emulsions prepared by the SPI-lentinan conjugates (ultrasound) as emulsifiers	1099:1179	Furthermore, the emulsions prepared by the SPI-lentinan conjugates (ultrasound) as emulsifiers possessed the highest stability against environmental stresses.					
32593796	6	52	contain	possessed	1181:1189	arg2	stability					1203:1211	the highest stability	1191:1211	the highest stability against environmental stresses	1191:1242	Furthermore, the emulsions prepared by the SPI-lentinan conjugates (ultrasound) as emulsifiers possessed the highest stability against environmental stresses.					
34582712	6	0	theme	enzymes	961:967	arg1	history					937:943	A first phylogenetic history	916:943	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope	916:1046	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	5	1	from	perspective	903:913	arg1	enzymes					874:880	these post-translational modifying enzymes	839:880	these post-translational modifying enzymes from an evolutionary perspective	839:913	Given the fundamental role played by DG in muscle stability, it is biochemically and clinically relevant to investigate these post-translational modifying enzymes from an evolutionary perspective.					
34582712	1	2	theme	pivotal	146:152	arg1	role					154:157	a pivotal role	144:157	a pivotal role for the stabilization of muscles	144:190	The dystroglycan (DG) complex plays a pivotal role for the stabilization of muscles in Metazoa.					
34582712	6	3	theme	epitope	1040:1046	arg1	fabrication					985:995	the fabrication	981:995	the fabrication of the so-called 'M3 core' laminin-binding epitope	981:1046	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	6	4	theme	comparison	1087:1096	arg1	approach					1098:1105	an overall sequence comparison approach	1067:1105	an overall sequence comparison approach	1067:1105	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	3	5	gly	glycosylated	398:409	arg1	subunit					375:381	The α-DG subunit	366:381	The α-DG subunit	366:381	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	3	5	gly	glycosylated	398:409	arg2	subunit					375:381	The α-DG subunit	366:381	The α-DG subunit	366:381	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	3	5	gly	glycosylated	398:409	arg1	steps					425:429	sequential steps	414:429	sequential steps by several specific enzymes	414:457	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	2	6	theme	complex	319:325	arg1	process					357:363	a complex post-translational maturation process	317:363	a complex post-translational maturation process	317:363	It is formed by two subunits, extracellular α-DG and transmembrane β-DG, originating from a unique precursor via a complex post-translational maturation process.					
34582712	6	7	theme	laminin-binding	1024:1038	arg1	epitope					1040:1046	the so-called 'M3 core' laminin-binding epitope	1000:1046	the so-called 'M3 core' laminin-binding epitope	1000:1046	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	7	8	theme	muscle	1496:1501	arg1	stability					1503:1511	skeletal muscle stability	1487:1511	skeletal muscle stability	1487:1511	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	6	9	from	details	1124:1130	arg1	set					1157:1159	the primordial enzyme set	1135:1159	the primordial enzyme set	1135:1159	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	0	10	theme	α-dystroglycan	92:105	arg1	glycoepitope					76:87	the laminin-binding glycoepitope	56:87	the laminin-binding glycoepitope of α-dystroglycan	56:105	High degree of conservation of the enzymes synthesizing the laminin-binding glycoepitope of α-dystroglycan.					
34582712	6	11	theme	core	1018:1021	arg1	epitope					1040:1046	the so-called 'M3 core' laminin-binding epitope	1000:1046	the so-called 'M3 core' laminin-binding epitope	1000:1046	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	7	12	theme	well-conserved	1270:1283	arg1	set					1295:1297	a well-conserved enzymatic set	1268:1297	a well-conserved enzymatic set responsible for the glycosylation of α-DG	1268:1339	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	2	13	theme	transmembrane	257:269	arg1	subunits					224:231	two subunits	220:231	two subunits	220:231	It is formed by two subunits, extracellular α-DG and transmembrane β-DG, originating from a unique precursor via a complex post-translational maturation process.					
34582712	2	13	theme	transmembrane	257:269	arg1	β-DG					271:274	transmembrane β-DG	257:274	transmembrane β-DG	257:274	It is formed by two subunits, extracellular α-DG and transmembrane β-DG, originating from a unique precursor via a complex post-translational maturation process.					
34582712	6	14	from	conservation	1198:1209	arg1	Metazoa					1214:1220	Metazoa	1214:1220	Metazoa	1214:1220	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	2	15	theme	unique	296:301	arg1	precursor					303:311	a unique precursor	294:311	a unique precursor via a complex post-translational maturation process	294:363	It is formed by two subunits, extracellular α-DG and transmembrane β-DG, originating from a unique precursor via a complex post-translational maturation process.					
34582712	7	16	theme	enzymatic	1285:1293	arg1	set					1295:1297	a well-conserved enzymatic set	1268:1297	a well-conserved enzymatic set responsible for the glycosylation of α-DG	1268:1339	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	6	17	theme	sequence	1078:1085	arg1	approach					1098:1105	an overall sequence comparison approach	1067:1105	an overall sequence comparison approach	1067:1105	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	4	18	theme	neuromuscular	654:666	arg1	disorders					668:676	severe neuromuscular disorders	647:676	severe neuromuscular disorders collectively named dystroglycanopathies	647:716	Mutations of several of these enzymes cause an alteration of the carbohydrate structure of α-DG, resulting in severe neuromuscular disorders collectively named dystroglycanopathies.					
34582712	5	19	theme	muscle	762:767	arg1	stability					769:777	muscle stability	762:777	muscle stability	762:777	Given the fundamental role played by DG in muscle stability, it is biochemically and clinically relevant to investigate these post-translational modifying enzymes from an evolutionary perspective.					
34582712	6	20	theme	phylogenetic	924:935	arg1	history					937:943	A first phylogenetic history	916:943	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope	916:1046	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	6	21	theme	substantial	1186:1196	arg1	conservation					1198:1209	substantial conservation	1186:1209	substantial conservation in Metazoa	1186:1220	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	3	22	theme	several	434:440	arg1	enzymes					451:457	several specific enzymes	434:457	several specific enzymes	434:457	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	3	23	theme	α-DG	370:373	arg1	subunit					375:381	The α-DG subunit	366:381	The α-DG subunit	366:381	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	3	24	theme	membrane	517:524	arg1	molecules					526:534	basement membrane molecules	508:534	basement membrane molecules	508:534	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	6	25	theme	interesting	1112:1122	arg1	details					1124:1130	interesting details	1112:1130	interesting details on the primordial enzyme set	1112:1159	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	3	26	theme	sequential	414:423	arg1	steps					425:429	sequential steps	414:429	sequential steps by several specific enzymes	414:457	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	0	27	theme	High	0:3	arg1	degree					5:10	High degree	0:10	High degree of conservation of the enzymes	0:41	High degree of conservation of the enzymes synthesizing the laminin-binding glycoepitope of α-dystroglycan.					
34582712	6	28	theme	M3	1015:1016	arg1	epitope					1040:1046	the so-called 'M3 core' laminin-binding epitope	1000:1046	the so-called 'M3 core' laminin-binding epitope	1000:1046	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	0	29	theme	conservation	15:26	arg1	degree					5:10	High degree	0:10	High degree of conservation of the enzymes	0:41	High degree of conservation of the enzymes synthesizing the laminin-binding glycoepitope of α-dystroglycan.					
34582712	4	30	theme	several	550:556	arg1	Mutations					537:545	Mutations	537:545	Mutations of several of these enzymes	537:573	Mutations of several of these enzymes cause an alteration of the carbohydrate structure of α-DG, resulting in severe neuromuscular disorders collectively named dystroglycanopathies.					
34582712	6	31	theme	so-called	1004:1012	arg1	epitope					1040:1046	the so-called 'M3 core' laminin-binding epitope	1000:1046	the so-called 'M3 core' laminin-binding epitope	1000:1046	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	7	32	theme	surrounding	1444:1454	arg1	membranes					1465:1473	surrounding basement membranes	1444:1473	surrounding basement membranes	1444:1473	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	1	33	theme	muscles	184:190	arg1	stabilization					167:179	the stabilization	163:179	the stabilization of muscles	163:190	The dystroglycan (DG) complex plays a pivotal role for the stabilization of muscles in Metazoa.					
34582712	5	34	theme	post-translational	845:862	arg1	enzymes					874:880	these post-translational modifying enzymes	839:880	these post-translational modifying enzymes from an evolutionary perspective	839:913	Given the fundamental role played by DG in muscle stability, it is biochemically and clinically relevant to investigate these post-translational modifying enzymes from an evolutionary perspective.					
34582712	4	35	theme	enzymes	567:573	arg1	several					550:556	several	550:556	several	550:556	Mutations of several of these enzymes cause an alteration of the carbohydrate structure of α-DG, resulting in severe neuromuscular disorders collectively named dystroglycanopathies.					
34582712	5	36	theme	fundamental	729:739	arg1	role					741:744	the fundamental role	725:744	the fundamental role played by DG in muscle stability	725:777	Given the fundamental role played by DG in muscle stability, it is biochemically and clinically relevant to investigate these post-translational modifying enzymes from an evolutionary perspective.					
34582712	1	37	theme	dystroglycan	112:123	arg1	complex					130:136	The dystroglycan (DG) complex	108:136	The dystroglycan (DG) complex	108:136	The dystroglycan (DG) complex plays a pivotal role for the stabilization of muscles in Metazoa.					
34582712	4	38	theme	α-DG	628:631	arg1	structure					615:623	the carbohydrate structure	598:623	the carbohydrate structure of α-DG	598:631	Mutations of several of these enzymes cause an alteration of the carbohydrate structure of α-DG, resulting in severe neuromuscular disorders collectively named dystroglycanopathies.					
34582712	6	39	theme	overall	1070:1076	arg1	approach					1098:1105	an overall sequence comparison approach	1067:1105	an overall sequence comparison approach	1067:1105	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	3	40	theme	specific	442:449	arg1	enzymes					451:457	several specific enzymes	434:457	several specific enzymes	434:457	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	5	41	theme	evolutionary	890:901	arg1	perspective					903:913	an evolutionary perspective	887:913	an evolutionary perspective	887:913	Given the fundamental role played by DG in muscle stability, it is biochemically and clinically relevant to investigate these post-translational modifying enzymes from an evolutionary perspective.					
34582712	2	42	theme	post-translational	327:344	arg1	process					357:363	a complex post-translational maturation process	317:363	a complex post-translational maturation process	317:363	It is formed by two subunits, extracellular α-DG and transmembrane β-DG, originating from a unique precursor via a complex post-translational maturation process.					
34582712	7	43	theme	α-DG	1336:1339	arg1	glycosylation					1319:1331	the glycosylation	1315:1331	the glycosylation of α-DG	1315:1339	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	7	44	gly	glycosylation	1319:1331	arg1	α-DG					1336:1339	α-DG	1336:1339	α-DG	1336:1339	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	6	45	theme	primordial	1139:1148	arg1	set					1157:1159	the primordial enzyme set	1135:1159	the primordial enzyme set	1135:1159	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	0	46	theme	enzymes	35:41	arg1	conservation					15:26	conservation	15:26	conservation of the enzymes	15:41	High degree of conservation of the enzymes synthesizing the laminin-binding glycoepitope of α-dystroglycan.					
34582712	7	47	theme	basement	1456:1463	arg1	membranes					1465:1473	surrounding basement membranes	1444:1473	surrounding basement membranes	1444:1473	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	7	48	theme	glycosylation	1372:1384	arg1	shell					1386:1390	the glycosylation shell	1368:1390	the glycosylation shell	1368:1390	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	6	49	theme	first	918:922	arg1	history					937:943	A first phylogenetic history	916:943	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope	916:1046	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	7	50	theme	set	1295:1297	arg1	evolution					1255:1263	the evolution	1251:1263	the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG	1251:1339	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	2	51	theme	extracellular	234:246	arg1	subunits					224:231	two subunits	220:231	two subunits	220:231	It is formed by two subunits, extracellular α-DG and transmembrane β-DG, originating from a unique precursor via a complex post-translational maturation process.					
34582712	2	51	theme	extracellular	234:246	arg1	α-DG					248:251	extracellular α-DG	234:251	extracellular α-DG	234:251	It is formed by two subunits, extracellular α-DG and transmembrane β-DG, originating from a unique precursor via a complex post-translational maturation process.					
34582712	3	52	theme	such	471:474	arg1	scaffold					483:490	such glycan scaffold	471:490	such glycan scaffold	471:490	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	6	53	theme	enzyme	1150:1155	arg1	set					1157:1159	the primordial enzyme set	1135:1159	the primordial enzyme set	1135:1159	A first phylogenetic history of the thirteen enzymes involved in the fabrication of the so-called 'M3 core' laminin-binding epitope has been traced by an overall sequence comparison approach, and interesting details on the primordial enzyme set have emerged, as well as substantial conservation in Metazoa.					
34582712	4	54	theme	carbohydrate	602:613	arg1	structure					615:623	the carbohydrate structure	598:623	the carbohydrate structure of α-DG	598:631	Mutations of several of these enzymes cause an alteration of the carbohydrate structure of α-DG, resulting in severe neuromuscular disorders collectively named dystroglycanopathies.					
34582712	7	55	theme	skeletal	1487:1494	arg1	stability					1503:1511	skeletal muscle stability	1487:1511	skeletal muscle stability	1487:1511	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	3	56	theme	glycan	476:481	arg1	scaffold					483:490	such glycan scaffold	471:490	such glycan scaffold	471:490	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	7	57	theme	responsible	1299:1309	arg1	set					1295:1297	a well-conserved enzymatic set	1268:1297	a well-conserved enzymatic set responsible for the glycosylation of α-DG	1268:1339	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	0	58	theme	laminin-binding	60:74	arg1	glycoepitope					76:87	the laminin-binding glycoepitope	56:87	the laminin-binding glycoepitope of α-dystroglycan	56:105	High degree of conservation of the enzymes synthesizing the laminin-binding glycoepitope of α-dystroglycan.					
34582712	7	59	theme	shell	1386:1390	arg1	importance					1354:1363	the importance	1350:1363	the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability	1350:1511	The optimization along with the evolution of a well-conserved enzymatic set responsible for the glycosylation of α-DG indicate the importance of the glycosylation shell in modulating the connection between sarcolemma and surrounding basement membranes to increase skeletal muscle stability, and eventually support movement and locomotion.					
34582712	5	60	theme	modifying	864:872	arg1	enzymes					874:880	these post-translational modifying enzymes	839:880	these post-translational modifying enzymes from an evolutionary perspective	839:913	Given the fundamental role played by DG in muscle stability, it is biochemically and clinically relevant to investigate these post-translational modifying enzymes from an evolutionary perspective.					
34582712	3	61	theme	basement	508:515	arg1	molecules					526:534	basement membrane molecules	508:534	basement membrane molecules	508:534	The α-DG subunit is extensively glycosylated in sequential steps by several specific enzymes and employs such glycan scaffold to tightly bind basement membrane molecules.					
34582712	4	62	theme	severe	647:652	arg1	disorders					668:676	severe neuromuscular disorders	647:676	severe neuromuscular disorders collectively named dystroglycanopathies	647:716	Mutations of several of these enzymes cause an alteration of the carbohydrate structure of α-DG, resulting in severe neuromuscular disorders collectively named dystroglycanopathies.					
34582712	2	63	theme	maturation	346:355	arg1	process					357:363	a complex post-translational maturation process	317:363	a complex post-translational maturation process	317:363	It is formed by two subunits, extracellular α-DG and transmembrane β-DG, originating from a unique precursor via a complex post-translational maturation process.					
34582712	4	64	theme	structure	615:623	arg1	alteration					584:593	an alteration	581:593	an alteration of the carbohydrate structure of α-DG	581:631	Mutations of several of these enzymes cause an alteration of the carbohydrate structure of α-DG, resulting in severe neuromuscular disorders collectively named dystroglycanopathies.					
33908046	11	0	theme	thermal	1509:1515	arg1	stability					1517:1525	thermal stability	1509:1525	thermal stability	1509:1525	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	4	1	theme	relative	637:644	arg1	humidity					646:653	50 °C and 75% relative humidity	623:653	50 °C and 75% relative humidity for 48 h	623:662	RESULTS This study characterized the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h.					
33908046	3	2	theme	structural	467:476	arg1	properties					493:502	physicochemical, structural and functional properties	450:502	physicochemical, structural and functional properties of silver carp Ms	450:520	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	8	3	theme	I	1135:1135	arg1	bands					1149:1153	the amide I, II and III bands	1125:1153	bands	1149:1153	Fourier transform infrared spectroscopy indicated that the amide I, II and III bands of Ms were changed by the glycation.					
33908046	6	4	theme	isoelectric	784:794	arg1	point					796:800	isoelectric point	784:800	isoelectric point (pI) reduced	784:813	Meanwhile, isoelectric point (pI) reduced, however, PKOGs showed no differences in pI.					
33908046	6	4	theme	isoelectric	784:794	arg1	pI					803:804	pI	803:804	pI	803:804	Meanwhile, isoelectric point (pI) reduced, however, PKOGs showed no differences in pI.					
33908046	6	4	theme	isoelectric	784:794	arg1	Meanwhile					773:781	Meanwhile	773:781	Meanwhile	773:781	Meanwhile, isoelectric point (pI) reduced, however, PKOGs showed no differences in pI.					
33908046	11	5	theme	resulting	1469:1477	arg1	improvement					1479:1489	the resulting improvement	1465:1489	the resulting improvement in solubility and thermal stability	1465:1525	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	1	6	theme	thermal	229:235	arg1	stability					237:245	thermal stability	229:245	thermal stability	229:245	BACKGROUND Myosin (Ms) is abundant in fish meat, but it has limited application in the food industry because of its low solubility and thermal stability.					
33908046	8	7	theme	amide	1129:1133	arg1	bands					1149:1153	the amide I, II and III bands	1125:1153	bands	1149:1153	Fourier transform infrared spectroscopy indicated that the amide I, II and III bands of Ms were changed by the glycation.					
33908046	7	8	theme	sulfate-polyacrylamide	875:896	arg1	electrophoresis					902:916	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	860:916	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis	860:925	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis suggested the formation of glycoconjugates, and scanning electron microscopy revealed thinner flakes and uneven appearance of glycoconjugates.					
33908046	7	9	theme	gel	898:900	arg1	electrophoresis					902:916	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	860:916	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis	860:925	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis suggested the formation of glycoconjugates, and scanning electron microscopy revealed thinner flakes and uneven appearance of glycoconjugates.					
33908046	3	10	theme	carp	514:517	arg1	Ms					519:520	silver carp Ms	507:520	silver carp Ms	507:520	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	5	11	theme	phosphorylation	678:692	arg1	degree					668:673	degree	668:673	degree of phosphorylation	668:692	As degree of phosphorylation increased, free amino content increased, whereas degree of grafting decreased.					
33908046	11	12	theme	Ms	1438:1439	arg1	modification					1441:1452	Ms modification	1438:1452	Ms modification	1438:1452	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	4	13	dep	humidity	646:653	arg1	%					635:635	%	635:635	%	635:635	RESULTS This study characterized the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h.					
33908046	1	14	from	meat	137:140	arg1	abundant					120:127	abundant	120:127	abundant	120:127	BACKGROUND Myosin (Ms) is abundant in fish meat, but it has limited application in the food industry because of its low solubility and thermal stability.					
33908046	3	15	theme	Ms	519:520	arg1	properties					493:502	physicochemical, structural and functional properties	450:502	physicochemical, structural and functional properties of silver carp Ms	450:520	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	9	16	with	NaCl	1232:1235	arg1	addition					1262:1269	increased phosphate addition	1242:1269	increased phosphate addition in PKOGs	1242:1278	Ms became highly soluble in 0.5 mol L-1 NaCl with increased phosphate addition in PKOGs.					
33908046	11	17	theme	CONCLUSION	1363:1372	arg1	Glycation					1374:1382	CONCLUSION Glycation	1363:1382	CONCLUSION Glycation with appropriate PKOG	1363:1404	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	11	17	theme	CONCLUSION	1363:1372	arg1	method					1427:1432	a promising method	1415:1432	a promising method for Ms modification	1415:1452	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	7	18	theme	electrophoresis	902:916	arg1	analysis					918:925	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis	860:925	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis	860:925	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis suggested the formation of glycoconjugates, and scanning electron microscopy revealed thinner flakes and uneven appearance of glycoconjugates.					
33908046	2	19	theme	previous	252:259	arg1	reports					261:267	Our previous reports	248:267	Our previous reports	248:267	Our previous reports found that these functional properties of Ms can be significantly improved after glycation with konjac oligo-glucomannan (KOG).					
33908046	10	20	theme	Thermal	1281:1287	arg1	stability					1289:1297	Thermal stability	1281:1297	Thermal stability of Ms	1281:1303	Thermal stability of Ms was effectively improved when heated at 80 °C for 60 min.					
33908046	11	21	with	Glycation	1374:1382	arg1	PKOG					1401:1404	appropriate PKOG	1389:1404	appropriate PKOG	1389:1404	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	8	22	theme	III	1145:1147	arg1	bands					1149:1153	the amide I, II and III bands	1125:1153	bands	1149:1153	Fourier transform infrared spectroscopy indicated that the amide I, II and III bands of Ms were changed by the glycation.					
33908046	9	23	from	addition	1262:1269	arg1	PKOGs					1274:1278	PKOGs	1274:1278	PKOGs	1274:1278	Ms became highly soluble in 0.5 mol L-1 NaCl with increased phosphate addition in PKOGs.					
33908046	1	24	theme	BACKGROUND	94:103	arg1	Ms					113:114	Ms	113:114	Ms	113:114	BACKGROUND Myosin (Ms) is abundant in fish meat, but it has limited application in the food industry because of its low solubility and thermal stability.					
33908046	1	24	theme	BACKGROUND	94:103	arg1	Myosin					105:110	BACKGROUND Myosin	94:110	BACKGROUND Myosin (Ms)	94:115	BACKGROUND Myosin (Ms) is abundant in fish meat, but it has limited application in the food industry because of its low solubility and thermal stability.					
33908046	5	25	theme	grafting	753:760	arg1	degree					743:748	degree	743:748	degree of grafting	743:760	As degree of phosphorylation increased, free amino content increased, whereas degree of grafting decreased.					
33908046	11	26	from	improvement	1479:1489	arg1	stability					1517:1525	thermal stability	1509:1525	thermal stability	1509:1525	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	11	26	from	improvement	1479:1489	arg1	solubility					1494:1503	solubility	1494:1503	solubility	1494:1503	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	7	27	theme	glycoconjugates	1053:1067	arg1	flakes					1021:1026	thinner flakes	1013:1026	thinner flakes	1013:1026	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis suggested the formation of glycoconjugates, and scanning electron microscopy revealed thinner flakes and uneven appearance of glycoconjugates.					
33908046	7	27	theme	glycoconjugates	1053:1067	arg1	appearance					1039:1048	uneven appearance	1032:1048	uneven appearance	1032:1048	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis suggested the formation of glycoconjugates, and scanning electron microscopy revealed thinner flakes and uneven appearance of glycoconjugates.					
33908046	6	28	from	differences	841:851	arg1	pI					856:857	pI	856:857	pI	856:857	Meanwhile, isoelectric point (pI) reduced, however, PKOGs showed no differences in pI.					
33908046	2	29	theme	konjac	365:370	arg1	oligo-glucomannan					372:388	konjac oligo-glucomannan	365:388	konjac oligo-glucomannan (KOG)	365:394	Our previous reports found that these functional properties of Ms can be significantly improved after glycation with konjac oligo-glucomannan (KOG).					
33908046	2	29	theme	konjac	365:370	arg1	KOG					391:393	KOG	391:393	KOG	391:393	Our previous reports found that these functional properties of Ms can be significantly improved after glycation with konjac oligo-glucomannan (KOG).					
33908046	2	30	theme	functional	286:295	arg1	properties					297:306	these functional properties	280:306	these functional properties of Ms	280:312	Our previous reports found that these functional properties of Ms can be significantly improved after glycation with konjac oligo-glucomannan (KOG).					
33908046	0	31	theme	carp	27:30	arg1	myosin					32:37	silver carp myosin	20:37	silver carp myosin	20:37	Characterization of silver carp myosin glycated with phosphorylated konjac oligo-glucomannan.					
33908046	1	32	from	abundant	120:127	arg1	meat					137:140	fish meat	132:140	fish meat	132:140	BACKGROUND Myosin (Ms) is abundant in fish meat, but it has limited application in the food industry because of its low solubility and thermal stability.					
33908046	8	33	dep	Fourier	1070:1076	arg1	transform					1078:1086	transform	1078:1086	transform infrared spectroscopy	1078:1108	Fourier transform infrared spectroscopy indicated that the amide I, II and III bands of Ms were changed by the glycation.					
33908046	0	34	theme	silver	20:25	arg1	carp					27:30	silver carp	20:30	silver carp myosin	20:37	Characterization of silver carp myosin glycated with phosphorylated konjac oligo-glucomannan.					
33908046	5	35	dep	increased	724:732	arg1	whereas					735:741	whereas	735:741	whereas	735:741	As degree of phosphorylation increased, free amino content increased, whereas degree of grafting decreased.					
33908046	11	36	theme	appropriate	1389:1399	arg1	PKOG					1401:1404	appropriate PKOG	1389:1404	appropriate PKOG	1389:1404	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	11	37	theme	promising	1417:1425	arg1	method					1427:1432	a promising method	1415:1432	a promising method for Ms modification	1415:1452	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	11	37	theme	promising	1417:1425	arg1	Glycation					1374:1382	CONCLUSION Glycation	1363:1382	CONCLUSION Glycation with appropriate PKOG	1363:1404	CONCLUSION Glycation with appropriate PKOG might be a promising method for Ms modification because of the resulting improvement in solubility and thermal stability.					
33908046	1	38	theme	food	181:184	arg1	industry					186:193	the food industry	177:193	the food industry	177:193	BACKGROUND Myosin (Ms) is abundant in fish meat, but it has limited application in the food industry because of its low solubility and thermal stability.					
33908046	8	39	dep	transform	1078:1086	arg1	infrared					1088:1095	infrared	1088:1095	transform infrared spectroscopy	1078:1108	Fourier transform infrared spectroscopy indicated that the amide I, II and III bands of Ms were changed by the glycation.					
33908046	3	40	theme	phosphorylated	421:434	arg1	KOG					436:438	phosphorylated KOG	421:438	phosphorylated KOG (PKOG)	421:445	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	3	40	theme	phosphorylated	421:434	arg1	PKOG					441:444	PKOG	441:444	PKOG	441:444	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	0	41	theme	myosin	32:37	arg1	Characterization					0:15	Characterization	0:15	Characterization of silver carp myosin	0:37	Characterization of silver carp myosin glycated with phosphorylated konjac oligo-glucomannan.					
33908046	4	42	dep	%	635:635	arg1	50 °C					623:627	50 °C	623:627	50 °C	623:627	RESULTS This study characterized the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h.					
33908046	4	42	dep	%	635:635	arg1	75					633:634	75	633:634	75	633:634	RESULTS This study characterized the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h.					
33908046	4	43	theme	Ms	590:591	arg1	protein					593:599	the silver carp Ms protein	574:599	the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h	574:662	RESULTS This study characterized the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h.					
33908046	7	44	theme	scanning	975:982	arg1	microscopy					993:1002	scanning electron microscopy	975:1002	scanning electron microscopy	975:1002	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis suggested the formation of glycoconjugates, and scanning electron microscopy revealed thinner flakes and uneven appearance of glycoconjugates.					
33908046	3	45	theme	KOG	436:438	arg1	effects					410:416	the effects	406:416	the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms	406:520	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	3	45	theme	KOG	436:438	arg1	unknown					532:538	unknown	532:538	unknown	532:538	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	0	46	theme	phosphorylated	53:66	arg1	oligo-glucomannan					75:91	phosphorylated konjac oligo-glucomannan	53:91	phosphorylated konjac oligo-glucomannan	53:91	Characterization of silver carp myosin glycated with phosphorylated konjac oligo-glucomannan.					
33908046	12	47	theme	Chemical	1546:1553	arg1	Industry					1555:1562	Chemical Industry	1546:1562	Chemical Industry	1546:1562	© 2021 Society of Chemical Industry.					
33908046	7	48	theme	thinner	1013:1019	arg1	flakes					1021:1026	thinner flakes	1013:1026	thinner flakes	1013:1026	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis suggested the formation of glycoconjugates, and scanning electron microscopy revealed thinner flakes and uneven appearance of glycoconjugates.					
33908046	4	49	dep	RESULTS	541:547	arg1	characterized					560:572	characterized	560:572	characterized the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h	560:662	RESULTS This study characterized the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h.					
33908046	7	50	theme	electron	984:991	arg1	microscopy					993:1002	scanning electron microscopy	975:1002	scanning electron microscopy	975:1002	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis suggested the formation of glycoconjugates, and scanning electron microscopy revealed thinner flakes and uneven appearance of glycoconjugates.					
33908046	3	51	theme	physicochemical	450:464	arg1	properties					493:502	physicochemical, structural and functional properties	450:502	physicochemical, structural and functional properties of silver carp Ms	450:520	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	7	52	theme	uneven	1032:1037	arg1	appearance					1039:1048	uneven appearance	1032:1048	uneven appearance	1032:1048	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis suggested the formation of glycoconjugates, and scanning electron microscopy revealed thinner flakes and uneven appearance of glycoconjugates.					
33908046	9	53	theme	phosphate	1252:1260	arg1	addition					1262:1269	increased phosphate addition	1242:1269	increased phosphate addition in PKOGs	1242:1278	Ms became highly soluble in 0.5 mol L-1 NaCl with increased phosphate addition in PKOGs.					
33908046	9	54	from	NaCl	1232:1235	arg1	soluble					1209:1215	soluble	1209:1215	soluble	1209:1215	Ms became highly soluble in 0.5 mol L-1 NaCl with increased phosphate addition in PKOGs.					
33908046	9	55	from	soluble	1209:1215	arg1	NaCl					1232:1235	0.5 mol L-1 NaCl	1220:1235	0.5 mol L-1 NaCl with increased phosphate addition in PKOGs	1220:1278	Ms became highly soluble in 0.5 mol L-1 NaCl with increased phosphate addition in PKOGs.					
33908046	9	56	theme	0.5 mol L-1	1220:1230	arg1	NaCl					1232:1235	0.5 mol L-1 NaCl	1220:1235	0.5 mol L-1 NaCl with increased phosphate addition in PKOGs	1220:1278	Ms became highly soluble in 0.5 mol L-1 NaCl with increased phosphate addition in PKOGs.					
33908046	5	57	theme	amino	710:714	arg1	content					716:722	free amino content	705:722	free amino content	705:722	As degree of phosphorylation increased, free amino content increased, whereas degree of grafting decreased.					
33908046	2	58	theme	Ms	311:312	arg1	properties					297:306	these functional properties	280:306	these functional properties of Ms	280:312	Our previous reports found that these functional properties of Ms can be significantly improved after glycation with konjac oligo-glucomannan (KOG).					
33908046	10	59	theme	Ms	1302:1303	arg1	stability					1289:1297	Thermal stability	1281:1297	Thermal stability of Ms	1281:1303	Thermal stability of Ms was effectively improved when heated at 80 °C for 60 min.					
33908046	4	60	theme	silver	578:583	arg1	protein					593:599	the silver carp Ms protein	574:599	the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h	574:662	RESULTS This study characterized the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h.					
33908046	3	61	theme	silver	507:512	arg1	carp					514:517	silver carp	507:517	silver carp Ms	507:520	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	2	62	with	glycation	350:358	arg1	oligo-glucomannan					372:388	konjac oligo-glucomannan	365:388	konjac oligo-glucomannan (KOG)	365:394	Our previous reports found that these functional properties of Ms can be significantly improved after glycation with konjac oligo-glucomannan (KOG).					
33908046	2	62	with	glycation	350:358	arg1	KOG					391:393	KOG	391:393	KOG	391:393	Our previous reports found that these functional properties of Ms can be significantly improved after glycation with konjac oligo-glucomannan (KOG).					
33908046	1	63	from	application	162:172	arg1	industry					186:193	the food industry	177:193	the food industry	177:193	BACKGROUND Myosin (Ms) is abundant in fish meat, but it has limited application in the food industry because of its low solubility and thermal stability.					
33908046	1	64	theme	fish	132:135	arg1	meat					137:140	fish meat	132:140	fish meat	132:140	BACKGROUND Myosin (Ms) is abundant in fish meat, but it has limited application in the food industry because of its low solubility and thermal stability.					
33908046	5	65	theme	free	705:708	arg1	content					716:722	free amino content	705:722	free amino content	705:722	As degree of phosphorylation increased, free amino content increased, whereas degree of grafting decreased.					
33908046	7	66	theme	glycoconjugates	954:968	arg1	formation					941:949	the formation	937:949	the formation of glycoconjugates	937:968	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis suggested the formation of glycoconjugates, and scanning electron microscopy revealed thinner flakes and uneven appearance of glycoconjugates.					
33908046	3	67	theme	functional	482:491	arg1	properties					493:502	physicochemical, structural and functional properties	450:502	physicochemical, structural and functional properties of silver carp Ms	450:520	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	1	68	theme	low	210:212	arg1	solubility					214:223	its low solubility	206:223	its low solubility	206:223	BACKGROUND Myosin (Ms) is abundant in fish meat, but it has limited application in the food industry because of its low solubility and thermal stability.					
33908046	0	69	theme	konjac	68:73	arg1	oligo-glucomannan					75:91	phosphorylated konjac oligo-glucomannan	53:91	phosphorylated konjac oligo-glucomannan	53:91	Characterization of silver carp myosin glycated with phosphorylated konjac oligo-glucomannan.					
33908046	9	70	theme	increased	1242:1250	arg1	addition					1262:1269	increased phosphate addition	1242:1269	increased phosphate addition in PKOGs	1242:1278	Ms became highly soluble in 0.5 mol L-1 NaCl with increased phosphate addition in PKOGs.					
33908046	3	71	from	effects	410:416	arg1	properties					493:502	physicochemical, structural and functional properties	450:502	physicochemical, structural and functional properties of silver carp Ms	450:520	However, the effects of phosphorylated KOG (PKOG) on physicochemical, structural and functional properties of silver carp Ms are still unknown.					
33908046	8	72	theme	Ms	1158:1159	arg1	bands					1149:1153	the amide I, II and III bands	1125:1153	bands	1149:1153	Fourier transform infrared spectroscopy indicated that the amide I, II and III bands of Ms were changed by the glycation.					
33908046	4	73	theme	carp	585:588	arg1	protein					593:599	the silver carp Ms protein	574:599	the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h	574:662	RESULTS This study characterized the silver carp Ms protein glycated with PKOG at 50 °C and 75% relative humidity for 48 h.					
32058627	2	0	theme	protective	504:513	arg1	mAb					515:517	a protective mAb	502:517	a protective mAb	502:517	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	4	1	theme	polysaccharide	904:917	arg1	complex					932:938	the polysaccharide fragment-mAb complex	900:938	the polysaccharide fragment-mAb complex	900:938	Here, starting from the X-ray crystallographic structure of the polysaccharide fragment-mAb complex, we synthesized a hexasaccharide comprising exclusively the relevant positions involved in binding.					
32058627	7	2	theme	crystallography-guided	1528:1549	arg1	design					1551:1556	an X-ray crystallography-guided design	1519:1556	an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine	1519:1608	This is the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine.					
32058627	6	3	theme	functional	1354:1363	arg1	response					1372:1379	a robust functional immune response	1345:1379	a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen	1345:1486	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	6	3	theme	functional	1354:1363	arg1	inferior					1394:1401	inferior	1394:1401	inferior	1394:1401	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	3	4	theme	reported	529:536	arg1	data					538:541	reported data	529:541	reported data	529:541	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	2	5	from	cause	343:347	arg1	newborns					372:379	newborns	372:379	newborns	372:379	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	6	6	theme	immune	1365:1370	arg1	response					1372:1379	a robust functional immune response	1345:1379	a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen	1345:1486	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	6	6	theme	immune	1365:1370	arg1	inferior					1394:1401	inferior	1394:1401	inferior	1394:1401	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	5	7	theme	plasmon	1070:1076	arg1	resonance					1078:1086	competitive surface plasmon resonance	1050:1086	competitive surface plasmon resonance	1050:1086	Combining competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.					
32058627	4	8	theme	complex	932:938	arg1	structure					887:895	the X-ray crystallographic structure	860:895	the X-ray crystallographic structure of the polysaccharide fragment-mAb complex	860:938	Here, starting from the X-ray crystallographic structure of the polysaccharide fragment-mAb complex, we synthesized a hexasaccharide comprising exclusively the relevant positions involved in binding.					
32058627	5	9	theme	difference	1112:1121	arg1	spectroscopy					1127:1138	competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy	1050:1138	competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling	1050:1168	Combining competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.					
32058627	4	10	theme	relevant	1000:1007	arg1	positions					1009:1017	the relevant positions	996:1017	the relevant positions involved in binding	996:1037	Here, starting from the X-ray crystallographic structure of the polysaccharide fragment-mAb complex, we synthesized a hexasaccharide comprising exclusively the relevant positions involved in binding.					
32058627	3	11	theme	proper	724:729	arg1	degree					745:750	a proper glycosylation degree	722:750	a proper glycosylation degree	722:750	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	4	12	theme	fragment-mAb	919:930	arg1	complex					932:938	the polysaccharide fragment-mAb complex	900:938	the polysaccharide fragment-mAb complex	900:938	Here, starting from the X-ray crystallographic structure of the polysaccharide fragment-mAb complex, we synthesized a hexasaccharide comprising exclusively the relevant positions involved in binding.					
32058627	3	13	theme	complex	566:572	arg1	epitope					574:580	a highly complex epitope	557:580	a highly complex epitope contained in a helical structure composed of more than four repeating units	557:656	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	3	14	theme	glycosylation	731:743	arg1	degree					745:750	a proper glycosylation degree	722:750	a proper glycosylation degree	722:750	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	4	15	theme	crystallographic	870:885	arg1	structure					887:895	the X-ray crystallographic structure	860:895	the X-ray crystallographic structure of the polysaccharide fragment-mAb complex	860:938	Here, starting from the X-ray crystallographic structure of the polysaccharide fragment-mAb complex, we synthesized a hexasaccharide comprising exclusively the relevant positions involved in binding.					
32058627	2	16	from	epitope	250:256	arg1	GBSIII					326:331	GBSIII	326:331	GBSIII	326:331	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	2	16	from	epitope	250:256	arg1	Streptococcus					311:323	Streptococcus	311:323	Streptococcus	311:323	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	2	16	from	epitope	250:256	arg1	cause					343:347	a major cause	335:347	a major cause of invasive disease in newborns	335:379	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	1	17	theme	glycoconjugate	191:204	arg1	vaccines					206:213	glycoconjugate vaccines	191:213	glycoconjugate vaccines	191:213	Identification of glycan functional epitopes is of paramount importance for rational design of glycoconjugate vaccines.					
32058627	4	18	theme	X-ray	864:868	arg1	structure					887:895	the X-ray crystallographic structure	860:895	the X-ray crystallographic structure of the polysaccharide fragment-mAb complex	860:938	Here, starting from the X-ray crystallographic structure of the polysaccharide fragment-mAb complex, we synthesized a hexasaccharide comprising exclusively the relevant positions involved in binding.					
32058627	5	19	theme	competitive	1050:1060	arg1	resonance					1078:1086	competitive surface plasmon resonance	1050:1086	competitive surface plasmon resonance	1050:1086	Combining competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.					
32058627	1	20	theme	vaccines	206:213	arg1	design					181:186	rational design	172:186	rational design of glycoconjugate vaccines	172:213	Identification of glycan functional epitopes is of paramount importance for rational design of glycoconjugate vaccines.					
32058627	5	21	theme	synthetic	1197:1205	arg1	glycan					1207:1212	this synthetic glycan	1192:1212	this synthetic glycan	1192:1212	Combining competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.					
32058627	6	22	theme	toxin	1329:1333	arg1	CRM197					1297:1302	CRM197	1297:1302	CRM197	1297:1302	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	6	22	theme	toxin	1329:1333	arg1	mutant					1308:1313	a mutant	1306:1313	a mutant of diphtheria toxin	1306:1333	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	2	23	theme	dimer	393:397	arg1	fragment					399:406	a dimer fragment	391:406	a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb	391:517	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	0	24	theme	Structure-Guided	0:15	arg1	Design					17:22	Structure-Guided Design	0:22	Structure-Guided Design of a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine	0:93	Structure-Guided Design of a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine.					
32058627	3	25	theme	functional	761:770	arg1	antibodies					772:781	functional antibodies	761:781	functional antibodies	761:781	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	6	26	theme	diphtheria	1318:1327	arg1	toxin					1329:1333	diphtheria toxin	1318:1333	diphtheria toxin	1318:1333	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	2	27	theme	major	337:341	arg1	Streptococcus					311:323	Streptococcus	311:323	Streptococcus	311:323	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	2	27	theme	major	337:341	arg1	cause					343:347	a major cause	335:347	a major cause of invasive disease in newborns	335:379	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	7	28	theme	conjugate	1592:1600	arg1	vaccine					1602:1608	a synthetic carbohydrate-based conjugate vaccine	1561:1608	a synthetic carbohydrate-based conjugate vaccine	1561:1608	This is the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine.					
32058627	6	29	theme	polysaccharide	1410:1423	arg1	conjugate					1425:1433	the polysaccharide conjugate	1406:1433	the polysaccharide conjugate	1406:1433	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	1	30	theme	functional	121:130	arg1	epitopes					132:139	glycan functional epitopes	114:139	glycan functional epitopes	114:139	Identification of glycan functional epitopes is of paramount importance for rational design of glycoconjugate vaccines.					
32058627	5	31	theme	saturation	1092:1101	arg1	transfer					1103:1110	saturation transfer	1092:1110	saturation transfer	1092:1110	Combining competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.					
32058627	2	32	theme	pentasaccharide	425:439	arg1	units					451:455	two pentasaccharide repeating units	421:455	two pentasaccharide repeating units	421:455	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	1	33	theme	glycan	114:119	arg1	epitopes					132:139	glycan functional epitopes	114:139	glycan functional epitopes	114:139	Identification of glycan functional epitopes is of paramount importance for rational design of glycoconjugate vaccines.					
32058627	5	34	theme	in-silico	1151:1159	arg1	modeling					1161:1168	in-silico modeling	1151:1168	competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling	1050:1168	Combining competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.					
32058627	1	35	theme	epitopes	132:139	arg1	Identification					96:109	Identification	96:109	Identification of glycan functional epitopes	96:139	Identification of glycan functional epitopes is of paramount importance for rational design of glycoconjugate vaccines.					
32058627	2	36	theme	capsular	265:272	arg1	polysaccharide					274:287	the capsular polysaccharide	261:287	the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns,	261:380	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	7	37	theme	first	1501:1505	arg1	evidence					1507:1514	the first evidence	1497:1514	the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine	1497:1608	This is the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine.					
32058627	7	37	theme	first	1501:1505	arg1	This					1489:1492	This	1489:1492	This	1489:1492	This is the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine.					
32058627	5	38	theme	transfer	1103:1110	arg1	spectroscopy					1127:1138	competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy	1050:1138	competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling	1050:1168	Combining competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.					
32058627	0	39	theme	Synthetic	60:68	arg1	Vaccine					87:93	a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine	27:93	a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine	27:93	Structure-Guided Design of a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine.					
32058627	3	40	theme	full-length	801:811	arg1	polysaccharide					824:837	the full-length conjugated polysaccharide	797:837	the full-length conjugated polysaccharide	797:837	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	3	41	theme	helical	597:603	arg1	structure					605:613	a helical structure	595:613	a helical structure composed of more than four repeating units	595:656	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	0	42	theme	Type III	51:58	arg1	Vaccine					87:93	a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine	27:93	a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine	27:93	Structure-Guided Design of a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine.					
32058627	6	43	theme	vaccine	1472:1478	arg1	antigen					1480:1486	a vaccine antigen	1470:1486	a vaccine antigen	1470:1486	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	2	44	theme	structural	239:248	arg1	epitope					250:256	the structural epitope	235:256	the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns,	235:380	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	5	45	theme	NMR	1123:1125	arg1	spectroscopy					1127:1138	competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy	1050:1138	competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling	1050:1168	Combining competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.					
32058627	2	46	theme	disease	361:367	arg1	Streptococcus					311:323	Streptococcus	311:323	Streptococcus	311:323	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	2	46	theme	disease	361:367	arg1	cause					343:347	a major cause	335:347	a major cause of invasive disease in newborns	335:379	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	7	47	theme	design	1551:1556	arg1	evidence					1507:1514	the first evidence	1497:1514	the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine	1497:1608	This is the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine.					
32058627	7	47	theme	design	1551:1556	arg1	This					1489:1492	This	1489:1492	This	1489:1492	This is the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine.					
32058627	7	48	theme	vaccine	1602:1608	arg1	design					1551:1556	an X-ray crystallography-guided design	1519:1556	an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine	1519:1608	This is the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine.					
32058627	5	49	theme	surface	1062:1068	arg1	resonance					1078:1086	competitive surface plasmon resonance	1050:1086	competitive surface plasmon resonance	1050:1086	Combining competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.					
32058627	1	50	theme	paramount	147:155	arg1	importance					157:166	paramount importance	147:166	paramount importance	147:166	Identification of glycan functional epitopes is of paramount importance for rational design of glycoconjugate vaccines.					
32058627	2	51	from	Streptococcus	311:323	arg1	epitope					250:256	the structural epitope	235:256	the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns,	235:380	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	2	51	from	Streptococcus	311:323	arg1	polysaccharide					274:287	the capsular polysaccharide	261:287	the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns,	261:380	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	7	52	theme	synthetic	1563:1571	arg1	vaccine					1602:1608	a synthetic carbohydrate-based conjugate vaccine	1561:1608	a synthetic carbohydrate-based conjugate vaccine	1561:1608	This is the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine.					
32058627	3	53	theme	repeating	642:650	arg1	units					652:656	more than four repeating units	627:656	more than four repeating units	627:656	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	2	54	theme	invasive	352:359	arg1	disease					361:367	invasive disease	352:367	invasive disease	352:367	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	0	55	theme	Vaccine	87:93	arg1	Design					17:22	Structure-Guided Design	0:22	Structure-Guided Design of a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine	0:93	Structure-Guided Design of a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine.					
32058627	3	56	theme	such	674:677	arg1	dimer					679:683	such dimer	674:683	such dimer conjugated to a carrier protein with a proper glycosylation degree	674:750	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	5	57	theme	resonance	1078:1086	arg1	spectroscopy					1127:1138	competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy	1050:1138	competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling	1050:1168	Combining competitive surface plasmon resonance and saturation transfer difference NMR spectroscopy as well as in-silico modeling, we demonstrated that this synthetic glycan was recognized by the mAb similarly to the dimer.					
32058627	2	58	theme	repeating	441:449	arg1	units					451:455	two pentasaccharide repeating units	421:455	two pentasaccharide repeating units	421:455	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	3	59	theme	conjugated	813:822	arg1	polysaccharide					824:837	the full-length conjugated polysaccharide	797:837	the full-length conjugated polysaccharide	797:837	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	0	60	theme	Glycan-Conjugate	70:85	arg1	Vaccine					87:93	a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine	27:93	a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine	27:93	Structure-Guided Design of a Group B Streptococcus Type III Synthetic Glycan-Conjugate Vaccine.					
32058627	3	61	theme	carrier	701:707	arg1	protein					709:715	a carrier protein	699:715	a carrier protein	699:715	Although reported data had suggested a highly complex epitope contained in a helical structure composed of more than four repeating units, we showed that such dimer conjugated to a carrier protein with a proper glycosylation degree elicited functional antibodies comparably to the full-length conjugated polysaccharide.					
32058627	2	62	theme	polysaccharide	274:287	arg1	epitope					250:256	the structural epitope	235:256	the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns,	235:380	We recently mapped the structural epitope of the capsular polysaccharide from type III Group B Streptococcus (GBSIII), a major cause of invasive disease in newborns, by using a dimer fragment (composed of two pentasaccharide repeating units) obtained by depolymerization complexed with a protective mAb.					
32058627	1	63	theme	rational	172:179	arg1	design					181:186	rational design	172:186	rational design of glycoconjugate vaccines	172:213	Identification of glycan functional epitopes is of paramount importance for rational design of glycoconjugate vaccines.					
32058627	6	64	theme	robust	1347:1352	arg1	response					1372:1379	a robust functional immune response	1345:1379	a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen	1345:1486	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	6	64	theme	robust	1347:1352	arg1	inferior					1394:1401	inferior	1394:1401	inferior	1394:1401	The hexasaccharide conjugated to CRM197 , a mutant of diphtheria toxin, elicited a robust functional immune response that was not inferior to the polysaccharide conjugate, indicating that it may suffice as a vaccine antigen.					
32058627	7	65	theme	carbohydrate-based	1573:1590	arg1	vaccine					1602:1608	a synthetic carbohydrate-based conjugate vaccine	1561:1608	a synthetic carbohydrate-based conjugate vaccine	1561:1608	This is the first evidence of an X-ray crystallography-guided design of a synthetic carbohydrate-based conjugate vaccine.					
32505675	1	0	contain	have	121:124	arg2	domains					146:152	large extracellular domains	126:152	large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells	126:295	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	1	0	contain	have	121:124	arg1	receptors					111:119	Notch receptors	105:119	Notch receptors	105:119	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	10	1	theme	disulfide	1516:1524	arg1	arrangement					1531:1541	the correct disulfide bond arrangement	1504:1541	the correct disulfide bond arrangement	1504:1541	With the fusion tag, EGF27 was refolded to produce the correct disulfide bond arrangement, which was verified enzymatically with the glycosyltransferases, Protein O-fucosyltransferase 1 (POFUT1) and Lunatic Fringe (LFNG).					
32505675	8	2	theme	steady	1229:1234	arg1	environment					1242:1252	a steady redox environment	1227:1252	a steady redox environment were unsuccessful due to low yields and extensive mixed-disulfide bond cross-linking	1227:1337	Previous attempts to express the repeat alone and refold the repeat under a steady redox environment were unsuccessful due to low yields and extensive mixed-disulfide bond cross-linking.					
32505675	4	3	theme	distinct	651:658	arg1	roles					660:664	distinct roles	651:664	distinct roles	651:664	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	0	4	theme	repeat	76:81	arg1	purification					12:23	purification	12:23	purification	12:23	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	0	4	theme	repeat	76:81	arg1	glycosylation					30:42	glycosylation	30:42	glycosylation	30:42	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	0	4	theme	repeat	76:81	arg1	Expression					0:9	Expression	0:9	Expression	0:9	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	6	5	from	role	921:924	arg1	regulation					958:967	regulation	958:967	regulation	958:967	To better understand the role of EGF27 in NOTCH1 function and regulation, the 3-dimensional structures of EGF27 and its glycoforms are being pursued.					
32505675	6	5	from	role	921:924	arg1	function					945:952	NOTCH1 function	938:952	NOTCH1 function	938:952	To better understand the role of EGF27 in NOTCH1 function and regulation, the 3-dimensional structures of EGF27 and its glycoforms are being pursued.					
32505675	9	6	theme	cleavable	1363:1371	arg1	tag					1404:1406	a cleavable maltose binding protein fusion tag	1361:1406	a cleavable maltose binding protein fusion tag	1361:1406	A new strategy using a cleavable maltose binding protein fusion tag increased the solubility and yield of EGF27.					
32505675	9	7	theme	EGF27	1446:1450	arg1	yield					1437:1441	yield	1437:1441	yield	1437:1441	A new strategy using a cleavable maltose binding protein fusion tag increased the solubility and yield of EGF27.					
32505675	9	7	theme	EGF27	1446:1450	arg1	solubility					1422:1431	solubility	1422:1431	solubility	1422:1431	A new strategy using a cleavable maltose binding protein fusion tag increased the solubility and yield of EGF27.					
32505675	1	8	theme	cell	242:245	arg1	signaling					247:255	cell signaling	242:255	cell signaling	242:255	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	0	9	theme	mouse	91:95	arg1	NOTCH1					97:102	mouse NOTCH1	91:102	mouse NOTCH1	91:102	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	5	10	from	NOTCH1	715:720	arg1	repeat					686:691	EGF repeat 27	682:694	EGF repeat 27 (EGF27) from mouse NOTCH1	682:720	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	5	10	from	NOTCH1	715:720	arg1	EGF27					697:701	EGF27	697:701	EGF27	697:701	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	10	11	theme	fusion	1462:1467	arg1	tag					1469:1471	the fusion tag	1458:1471	the fusion tag	1458:1471	With the fusion tag, EGF27 was refolded to produce the correct disulfide bond arrangement, which was verified enzymatically with the glycosyltransferases, Protein O-fucosyltransferase 1 (POFUT1) and Lunatic Fringe (LFNG).					
32505675	5	12	theme	specific	805:812	arg1	enzymes					821:827	specific Fringe enzymes	805:827	specific Fringe enzymes	805:827	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	5	13	theme	EGF	682:684	arg1	repeat					686:691	EGF repeat 27	682:694	EGF repeat 27 (EGF27) from mouse NOTCH1	682:720	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	5	13	theme	EGF	682:684	arg1	EGF27					697:701	EGF27	697:701	EGF27	697:701	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	5	14	theme	ligand	880:885	arg1	binding					887:893	ligand binding	880:893	ligand binding	880:893	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	7	15	theme	coli	1049:1052	arg1	cells					1054:1058	E. coli cells	1046:1058	E. coli cells	1046:1058	E. coli cells were used to produce EGF27 in sufficient quantities for nuclear magnetic resonance analysis.					
32505675	5	16	theme	Fringe	814:819	arg1	enzymes					821:827	specific Fringe enzymes	805:827	specific Fringe enzymes	805:827	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	6	17	theme	glycoforms	1016:1025	arg1	structures					988:997	the 3-dimensional structures	970:997	the 3-dimensional structures of EGF27 and its glycoforms	970:1025	To better understand the role of EGF27 in NOTCH1 function and regulation, the 3-dimensional structures of EGF27 and its glycoforms are being pursued.					
32505675	1	18	contain	containing	154:163	arg1	domains					146:152	large extracellular domains	126:152	large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells	126:295	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	1	18	contain	containing	154:163	arg2	repeats					216:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	6	19	theme	EGF27	1002:1006	arg1	structures					988:997	the 3-dimensional structures	970:997	the 3-dimensional structures of EGF27 and its glycoforms	970:1025	To better understand the role of EGF27 in NOTCH1 function and regulation, the 3-dimensional structures of EGF27 and its glycoforms are being pursued.					
32505675	9	20	theme	new	1342:1344	arg1	strategy					1346:1353	A new strategy	1340:1353	A new strategy using a cleavable maltose binding protein fusion tag	1340:1406	A new strategy using a cleavable maltose binding protein fusion tag increased the solubility and yield of EGF27.					
32505675	3	21	theme	EGF	412:414	arg1	repeat					416:421	Each EGF repeat	407:421	Each EGF repeat	407:421	Each EGF repeat consists of about 40 amino acids with 3 conserved disulfide bonds.					
32505675	2	22	theme	cell	386:389	arg1	decisions					396:404	cell fate decisions	386:404	cell fate decisions	386:404	Notch receptors play major roles in a variety of developmental processes by controlling cell fate decisions.					
32505675	9	23	theme	maltose	1373:1379	arg1	tag					1404:1406	a cleavable maltose binding protein fusion tag	1361:1406	a cleavable maltose binding protein fusion tag	1361:1406	A new strategy using a cleavable maltose binding protein fusion tag increased the solubility and yield of EGF27.					
32505675	10	24	theme	Protein	1608:1614	arg1	O-fucosyltransferase					1616:1635	Protein O-fucosyltransferase 1	1608:1637	Protein O-fucosyltransferase 1 (POFUT1)	1608:1646	With the fusion tag, EGF27 was refolded to produce the correct disulfide bond arrangement, which was verified enzymatically with the glycosyltransferases, Protein O-fucosyltransferase 1 (POFUT1) and Lunatic Fringe (LFNG).					
32505675	10	24	theme	Protein	1608:1614	arg1	POFUT1					1640:1645	POFUT1	1640:1645	POFUT1	1640:1645	With the fusion tag, EGF27 was refolded to produce the correct disulfide bond arrangement, which was verified enzymatically with the glycosyltransferases, Protein O-fucosyltransferase 1 (POFUT1) and Lunatic Fringe (LFNG).					
32505675	10	24	theme	Protein	1608:1614	arg1	glycosyltransferases					1586:1605	the glycosyltransferases	1582:1605	the glycosyltransferases	1582:1605	With the fusion tag, EGF27 was refolded to produce the correct disulfide bond arrangement, which was verified enzymatically with the glycosyltransferases, Protein O-fucosyltransferase 1 (POFUT1) and Lunatic Fringe (LFNG).					
32505675	5	25	theme	mouse	709:713	arg1	NOTCH1					715:720	mouse NOTCH1	709:720	mouse NOTCH1	709:720	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	7	26	theme	sufficient	1090:1099	arg1	quantities					1101:1110	sufficient quantities	1090:1110	sufficient quantities for nuclear magnetic resonance analysis	1090:1150	E. coli cells were used to produce EGF27 in sufficient quantities for nuclear magnetic resonance analysis.					
32505675	3	27	theme	conserved	463:471	arg1	bonds					483:487	3 conserved disulfide bonds	461:487	3 conserved disulfide bonds	461:487	Each EGF repeat consists of about 40 amino acids with 3 conserved disulfide bonds.					
32505675	6	28	theme	3-dimensional	974:986	arg1	structures					988:997	the 3-dimensional structures	970:997	the 3-dimensional structures of EGF27 and its glycoforms	970:1025	To better understand the role of EGF27 in NOTCH1 function and regulation, the 3-dimensional structures of EGF27 and its glycoforms are being pursued.					
32505675	4	29	theme	O-linked	530:537	arg1	glycans					546:552	O-linked fucose glycans	530:552	O-linked fucose glycans	530:552	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	7	30	theme	nuclear	1116:1122	arg1	resonance					1133:1141	nuclear magnetic resonance	1116:1141	nuclear magnetic resonance analysis	1116:1150	E. coli cells were used to produce EGF27 in sufficient quantities for nuclear magnetic resonance analysis.					
32505675	7	31	theme	magnetic	1124:1131	arg1	resonance					1133:1141	nuclear magnetic resonance	1116:1141	nuclear magnetic resonance analysis	1116:1150	E. coli cells were used to produce EGF27 in sufficient quantities for nuclear magnetic resonance analysis.					
32505675	1	32	theme	tandem	174:179	arg1	repeats					216:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	10	33	theme	correct	1508:1514	arg1	arrangement					1531:1541	the correct disulfide bond arrangement	1504:1541	the correct disulfide bond arrangement	1504:1541	With the fusion tag, EGF27 was refolded to produce the correct disulfide bond arrangement, which was verified enzymatically with the glycosyltransferases, Protein O-fucosyltransferase 1 (POFUT1) and Lunatic Fringe (LFNG).					
32505675	1	34	from	ligands	268:274	arg1	cells					291:295	neighboring cells	279:295	neighboring cells	279:295	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	2	35	theme	Notch	298:302	arg1	receptors					304:312	Notch receptors	298:312	Notch receptors	298:312	Notch receptors play major roles in a variety of developmental processes by controlling cell fate decisions.					
32505675	1	36	theme	epidermal	181:189	arg1	repeats					216:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	7	37	used	used	1065:1068	arg2	cells					1054:1058	E. coli cells	1046:1058	E. coli cells	1046:1058	E. coli cells were used to produce EGF27 in sufficient quantities for nuclear magnetic resonance analysis.					
32505675	8	38	theme	mixed-disulfide	1304:1318	arg1	cross-linking					1325:1337	extensive mixed-disulfide bond cross-linking	1294:1337	extensive mixed-disulfide bond cross-linking	1294:1337	Previous attempts to express the repeat alone and refold the repeat under a steady redox environment were unsuccessful due to low yields and extensive mixed-disulfide bond cross-linking.					
32505675	5	39	theme	NOTCH1	858:863	arg1	activation					865:874	NOTCH1 activation	858:874	NOTCH1 activation	858:874	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	1	40	theme	growth	191:196	arg1	repeats					216:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	4	41	theme	fucose	539:544	arg1	glycans					546:552	O-linked fucose glycans	530:552	O-linked fucose glycans	530:552	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	2	42	theme	developmental	347:359	arg1	processes					361:369	developmental processes	347:369	developmental processes	347:369	Notch receptors play major roles in a variety of developmental processes by controlling cell fate decisions.					
32505675	8	43	theme	low	1279:1281	arg1	yields					1283:1288	low yields	1279:1288	low yields	1279:1288	Previous attempts to express the repeat alone and refold the repeat under a steady redox environment were unsuccessful due to low yields and extensive mixed-disulfide bond cross-linking.					
32505675	1	44	theme	factor-like	198:208	arg1	repeats					216:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	5	45	mod	modified	725:732	arg1	repeat					686:691	EGF repeat 27	682:694	EGF repeat 27 (EGF27) from mouse NOTCH1	682:720	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	5	45	mod	modified	725:732	arg1	EGF27					697:701	EGF27	697:701	EGF27	697:701	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	5	45	mod	modified	725:732	arg3	O-fucose					739:746	O-fucose	739:746	O-fucose	739:746	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	1	46	theme	neighboring	279:289	arg1	cells					291:295	neighboring cells	279:295	neighboring cells	279:295	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	9	47	dep	solubility	1422:1431	arg1	the					1418:1420	the	1418:1420	the	1418:1420	A new strategy using a cleavable maltose binding protein fusion tag increased the solubility and yield of EGF27.					
32505675	8	48	theme	extensive	1294:1302	arg1	cross-linking					1325:1337	extensive mixed-disulfide bond cross-linking	1294:1337	extensive mixed-disulfide bond cross-linking	1294:1337	Previous attempts to express the repeat alone and refold the repeat under a steady redox environment were unsuccessful due to low yields and extensive mixed-disulfide bond cross-linking.					
32505675	2	49	theme	processes	361:369	arg1	variety					336:342	a variety	334:342	a variety of developmental processes	334:369	Notch receptors play major roles in a variety of developmental processes by controlling cell fate decisions.					
32505675	2	49	theme	processes	361:369	arg1	processes					361:369	developmental processes	347:369	developmental processes	347:369	Notch receptors play major roles in a variety of developmental processes by controlling cell fate decisions.					
32505675	9	50	theme	protein	1389:1395	arg1	tag					1404:1406	a cleavable maltose binding protein fusion tag	1361:1406	a cleavable maltose binding protein fusion tag	1361:1406	A new strategy using a cleavable maltose binding protein fusion tag increased the solubility and yield of EGF27.					
32505675	5	51	mod	modified	793:800	arg1	repeats					762:768	1 of 7 repeats	755:768	1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding	755:893	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	5	51	mod	modified	793:800	arg3	enzymes					821:827	specific Fringe enzymes	805:827	specific Fringe enzymes	805:827	EGF repeat 27 (EGF27) from mouse NOTCH1 is modified with O-fucose and is 1 of 7 repeats that is differentially modified by specific Fringe enzymes, which are known to regulate NOTCH1 activation and ligand binding.					
32505675	4	52	theme	EGF	502:504	arg1	repeats					506:512	the EGF repeats	498:512	the EGF repeats	498:512	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	10	53	theme	bond	1526:1529	arg1	arrangement					1531:1541	the correct disulfide bond arrangement	1504:1541	the correct disulfide bond arrangement	1504:1541	With the fusion tag, EGF27 was refolded to produce the correct disulfide bond arrangement, which was verified enzymatically with the glycosyltransferases, Protein O-fucosyltransferase 1 (POFUT1) and Lunatic Fringe (LFNG).					
32505675	9	54	theme	fusion	1397:1402	arg1	tag					1404:1406	a cleavable maltose binding protein fusion tag	1361:1406	a cleavable maltose binding protein fusion tag	1361:1406	A new strategy using a cleavable maltose binding protein fusion tag increased the solubility and yield of EGF27.					
32505675	1	55	theme	EGF	211:213	arg1	repeats					216:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	up to 36 tandem epidermal growth factor-like (EGF) repeats	165:222	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	0	56	theme	epidermal	47:55	arg1	repeat					76:81	epidermal growth factor-like repeat 27	47:84	epidermal growth factor-like repeat 27	47:84	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	4	57	theme	EGF	627:629	arg1	repeats					631:637	the EGF repeats	623:637	the EGF repeats	623:637	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	6	58	theme	NOTCH1	938:943	arg1	function					945:952	NOTCH1 function	938:952	NOTCH1 function	938:952	To better understand the role of EGF27 in NOTCH1 function and regulation, the 3-dimensional structures of EGF27 and its glycoforms are being pursued.					
32505675	7	59	theme	resonance	1133:1141	arg1	analysis					1143:1150	nuclear magnetic resonance analysis	1116:1150	nuclear magnetic resonance analysis	1116:1150	E. coli cells were used to produce EGF27 in sufficient quantities for nuclear magnetic resonance analysis.					
32505675	8	60	theme	bond	1320:1323	arg1	cross-linking					1325:1337	extensive mixed-disulfide bond cross-linking	1294:1337	extensive mixed-disulfide bond cross-linking	1294:1337	Previous attempts to express the repeat alone and refold the repeat under a steady redox environment were unsuccessful due to low yields and extensive mixed-disulfide bond cross-linking.					
32505675	1	61	theme	Notch	105:109	arg1	receptors					111:119	Notch receptors	105:119	Notch receptors	105:119	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	7	62	theme	E.	1046:1047	arg1	cells					1054:1058	E. coli cells	1046:1058	E. coli cells	1046:1058	E. coli cells were used to produce EGF27 in sufficient quantities for nuclear magnetic resonance analysis.					
32505675	4	63	mod	modified	518:525	arg3	glycans					546:552	O-linked fucose glycans	530:552	O-linked fucose glycans	530:552	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	4	63	mod	modified	518:525	arg1	repeats					506:512	the EGF repeats	498:512	the EGF repeats	498:512	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	4	63	mod	modified	518:525	arg1	Many					490:493	Many	490:493	Many	490:493	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	1	64	dep	36	171:172	arg1	to					168:169	to	168:169	to	168:169	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	9	65	theme	binding	1381:1387	arg1	tag					1404:1406	a cleavable maltose binding protein fusion tag	1361:1406	a cleavable maltose binding protein fusion tag	1361:1406	A new strategy using a cleavable maltose binding protein fusion tag increased the solubility and yield of EGF27.					
32505675	3	66	theme	disulfide	473:481	arg1	bonds					483:487	3 conserved disulfide bonds	461:487	3 conserved disulfide bonds	461:487	Each EGF repeat consists of about 40 amino acids with 3 conserved disulfide bonds.					
32505675	4	67	theme	repeats	506:512	arg1	repeats					506:512	the EGF repeats	498:512	the EGF repeats	498:512	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	4	67	theme	repeats	506:512	arg1	Many					490:493	Many	490:493	Many	490:493	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	4	68	link	O-linked	530:537	arg1	glycans					546:552	O-linked fucose glycans	530:552	O-linked fucose glycans	530:552	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	0	69	from	NOTCH1	97:102	arg1	purification					12:23	purification	12:23	purification	12:23	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	0	69	from	NOTCH1	97:102	arg1	glycosylation					30:42	glycosylation	30:42	glycosylation	30:42	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	0	69	from	NOTCH1	97:102	arg1	Expression					0:9	Expression	0:9	Expression	0:9	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	4	70	contain	have	574:577	arg1	repeats					506:512	the EGF repeats	498:512	the EGF repeats	498:512	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	4	70	contain	have	574:577	arg2	sites					595:599	calcium-binding sites	579:599	calcium-binding sites	579:599	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	4	70	contain	have	574:577	arg1	Many					490:493	Many	490:493	Many	490:493	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	2	71	theme	major	319:323	arg1	roles					325:329	major roles	319:329	major roles	319:329	Notch receptors play major roles in a variety of developmental processes by controlling cell fate decisions.					
32505675	10	72	theme	Lunatic	1652:1658	arg1	LFNG					1668:1671	LFNG	1668:1671	LFNG	1668:1671	With the fusion tag, EGF27 was refolded to produce the correct disulfide bond arrangement, which was verified enzymatically with the glycosyltransferases, Protein O-fucosyltransferase 1 (POFUT1) and Lunatic Fringe (LFNG).					
32505675	10	72	theme	Lunatic	1652:1658	arg1	Fringe					1660:1665	Lunatic Fringe	1652:1665	Lunatic Fringe (LFNG)	1652:1672	With the fusion tag, EGF27 was refolded to produce the correct disulfide bond arrangement, which was verified enzymatically with the glycosyltransferases, Protein O-fucosyltransferase 1 (POFUT1) and Lunatic Fringe (LFNG).					
32505675	10	72	theme	Lunatic	1652:1658	arg1	glycosyltransferases					1586:1605	the glycosyltransferases	1582:1605	the glycosyltransferases	1582:1605	With the fusion tag, EGF27 was refolded to produce the correct disulfide bond arrangement, which was verified enzymatically with the glycosyltransferases, Protein O-fucosyltransferase 1 (POFUT1) and Lunatic Fringe (LFNG).					
32505675	0	73	gly	glycosylation	30:42	arg1	repeat					76:81	epidermal growth factor-like repeat 27	47:84	epidermal growth factor-like repeat 27	47:84	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	1	74	theme	large	126:130	arg1	domains					146:152	large extracellular domains	126:152	large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells	126:295	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	8	75	theme	redox	1236:1240	arg1	environment					1242:1252	a steady redox environment	1227:1252	a steady redox environment were unsuccessful due to low yields and extensive mixed-disulfide bond cross-linking	1227:1337	Previous attempts to express the repeat alone and refold the repeat under a steady redox environment were unsuccessful due to low yields and extensive mixed-disulfide bond cross-linking.					
32505675	6	76	theme	EGF27	929:933	arg1	role					921:924	the role	917:924	the role of EGF27 in NOTCH1 function and regulation	917:967	To better understand the role of EGF27 in NOTCH1 function and regulation, the 3-dimensional structures of EGF27 and its glycoforms are being pursued.					
32505675	0	77	theme	factor-like	64:74	arg1	repeat					76:81	epidermal growth factor-like repeat 27	47:84	epidermal growth factor-like repeat 27	47:84	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	2	78	theme	fate	391:394	arg1	decisions					396:404	cell fate decisions	386:404	cell fate decisions	386:404	Notch receptors play major roles in a variety of developmental processes by controlling cell fate decisions.					
32505675	3	79	theme	amino	444:448	arg1	acids					450:454	about 40 amino acids	435:454	about 40 amino acids	435:454	Each EGF repeat consists of about 40 amino acids with 3 conserved disulfide bonds.					
32505675	1	80	theme	extracellular	132:144	arg1	domains					146:152	large extracellular domains	126:152	large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells	126:295	Notch receptors have large extracellular domains containing up to 36 tandem epidermal growth factor-like (EGF) repeats, which facilitate cell signaling by binding ligands on neighboring cells.					
32505675	4	81	theme	calcium-binding	579:593	arg1	sites					595:599	calcium-binding sites	579:599	calcium-binding sites	579:599	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
32505675	0	82	theme	growth	57:62	arg1	repeat					76:81	epidermal growth factor-like repeat 27	47:84	epidermal growth factor-like repeat 27	47:84	Expression, purification, and glycosylation of epidermal growth factor-like repeat 27 from mouse NOTCH1.					
32505675	4	83	theme	repeats	631:637	arg1	sequences					610:618	the sequences	606:618	the sequences of the EGF repeats	606:637	Many of the EGF repeats are modified by O-linked fucose glycans, and more than half have calcium-binding sites, but the sequences of the EGF repeats vary giving distinct roles to each repeat.					
33756033	3	0	theme	central	313:319	arg1	segments					334:341	The central glycopeptide segments	309:341	The central glycopeptide segments	309:341	The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans.					
33756033	3	1	theme	glycopeptide	321:332	arg1	segments					334:341	The central glycopeptide segments	309:341	The central glycopeptide segments	309:341	The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans.					
33756033	4	2	theme	plasma	546:551	arg1	clearance					553:561	in vivo plasma clearance	538:561	in vivo plasma clearance	538:561	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	3	3	theme	pseudoproline-assisted	361:382	arg1	aspartylation					393:405	pseudoproline-assisted Lansbury aspartylation	361:405	pseudoproline-assisted Lansbury aspartylation	361:405	The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans.					
33756033	6	4	theme	IL-6	853:856	arg1	glycoforms					839:848	the 2,6-sialylated glycoforms	820:848	the 2,6-sialylated glycoforms of IL-6	820:856	The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.					
33756033	4	5	gly	glycoforms	475:484	arg1	IL-6					470:473	Nine IL-6 glycoforms	465:484	Nine IL-6 glycoforms	465:484	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	4	6	dep	synthesized	491:501	arg1	evaluated					524:532	evaluated	524:532	evaluated for in vivo plasma clearance in rats	524:569	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	4	6	dep	synthesized	491:501	arg1	compared					575:582	compared	575:582	compared to non-glycosylated recombinant IL-6 from E. coli	575:632	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	3	7	theme	Lansbury	384:391	arg1	aspartylation					393:405	pseudoproline-assisted Lansbury aspartylation	361:405	pseudoproline-assisted Lansbury aspartylation	361:405	The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans.					
33756033	4	8	theme	which	513:517	arg1	which					513:517	which	513:517	which	513:517	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	4	8	theme	which	513:517	arg1	seven					504:508	seven	504:508	seven	504:508	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	5	9	theme	IL-6	640:643	arg1	glycoform					645:653	Each IL-6 glycoform	635:653	Each IL-6 glycoform	635:653	Each IL-6 glycoform was tested in three animals and reproducibly showed individual serum clearances depending on the structure of the N-glycan.					
33756033	0	10	theme	Natural	0:6	arg1	Glycoforms					8:17	Natural Glycoforms	0:17	Natural Glycoforms of Human Interleukin 6	0:40	Natural Glycoforms of Human Interleukin 6 Show Atypical Plasma Clearance.					
33756033	7	11	from	presence	1027:1034	arg1	cases					1082:1086	most cases	1077:1086	most cases	1077:1086	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	1	12	gly	glycoforms	87:96	arg1	interleukin					107:117	human interleukin 6	101:119	human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans	101:170	A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans was generated by semisynthesis.					
33756033	1	12	gly	glycoforms	87:96	arg1	IL-6					122:125	IL-6	122:125	IL-6	122:125	A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans was generated by semisynthesis.					
33756033	6	13	theme	2,6-sialylated	824:837	arg1	glycoforms					839:848	the 2,6-sialylated glycoforms	820:848	the 2,6-sialylated glycoforms of IL-6	820:856	The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.					
33756033	2	14	theme	chemical	259:266	arg1	ligation					268:275	sequential native chemical ligation	241:275	sequential native chemical ligation followed by two-step refolding	241:306	The three segments were connected by sequential native chemical ligation followed by two-step refolding.					
33756033	6	15	gly	glycoforms	839:848	arg1	IL-6					853:856	IL-6	853:856	IL-6	853:856	The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.					
33756033	1	16	theme	mannosidic	151:160	arg1	N-glycans					162:170	mannosidic N-glycans	151:170	mannosidic N-glycans	151:170	A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans was generated by semisynthesis.					
33756033	3	17	theme	subsequent	411:420	arg1	elongation					432:441	subsequent enzymatic elongation	411:441	subsequent enzymatic elongation	411:441	The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans.					
33756033	7	18	attach	presence	1027:1034	arg2	N-glycans					1064:1072	larger and multibranched N-glycans	1039:1072	larger and multibranched N-glycans	1039:1072	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	7	18	attach	presence	1027:1034	arg1	cases					1082:1086	most cases	1077:1086	most cases	1077:1086	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	7	19	theme	glycoforms	997:1006	arg1	clearance					979:987	the plasma clearance	968:987	the plasma clearance of IL-6 glycoforms	968:1006	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	3	20	theme	enzymatic	422:430	arg1	elongation					432:441	subsequent enzymatic elongation	411:441	subsequent enzymatic elongation	411:441	The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans.					
33756033	7	21	gly	non-glycosylated	946:961	arg1	IL-6					963:966	non-glycosylated IL-6	946:966	non-glycosylated IL-6	946:966	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	0	22	theme	Human	22:26	arg1	Interleukin					28:38	Human Interleukin 6	22:40	Human Interleukin 6	22:40	Natural Glycoforms of Human Interleukin 6 Show Atypical Plasma Clearance.					
33756033	6	23	gly	2,6-sialylated	824:837	arg1	IL-6					853:856	IL-6	853:856	IL-6	853:856	The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.					
33756033	6	23	gly	2,6-sialylated	824:837	arg1	glycoforms					839:848	the 2,6-sialylated glycoforms	820:848	the 2,6-sialylated glycoforms of IL-6	820:856	The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.					
33756033	1	24	theme	glycoforms	87:96	arg1	library					76:82	A library	74:82	A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans	74:170	A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans was generated by semisynthesis.					
33756033	4	25	theme	recombinant	604:614	arg1	IL-6					616:619	non-glycosylated recombinant IL-6	587:619	non-glycosylated recombinant IL-6	587:619	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	7	26	theme	multibranched	1050:1062	arg1	N-glycans					1064:1072	larger and multibranched N-glycans	1039:1072	larger and multibranched N-glycans	1039:1072	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	6	27	theme	terminal	913:920	arg1	galactoses					922:931	terminal galactoses	913:931	terminal galactoses	913:931	The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.					
33756033	2	28	theme	two-step	289:296	arg1	refolding					298:306	two-step refolding	289:306	two-step refolding	289:306	The three segments were connected by sequential native chemical ligation followed by two-step refolding.					
33756033	3	29	theme	complex	446:452	arg1	N-glycans					454:462	complex N-glycans	446:462	complex N-glycans	446:462	The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans.					
33756033	4	30	theme	non-glycosylated	587:602	arg1	IL-6					616:619	non-glycosylated recombinant IL-6	587:619	non-glycosylated recombinant IL-6	587:619	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	0	31	theme	Interleukin	28:38	arg1	Glycoforms					8:17	Natural Glycoforms	0:17	Natural Glycoforms of Human Interleukin 6	0:40	Natural Glycoforms of Human Interleukin 6 Show Atypical Plasma Clearance.					
33756033	3	32	theme	N-glycans	454:462	arg1	aspartylation					393:405	pseudoproline-assisted Lansbury aspartylation	361:405	pseudoproline-assisted Lansbury aspartylation	361:405	The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans.					
33756033	3	32	theme	N-glycans	454:462	arg1	elongation					432:441	subsequent enzymatic elongation	411:441	subsequent enzymatic elongation	411:441	The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans.					
33756033	0	33	theme	Atypical	47:54	arg1	Clearance					63:71	Atypical Plasma Clearance	47:71	Atypical Plasma Clearance	47:71	Natural Glycoforms of Human Interleukin 6 Show Atypical Plasma Clearance.					
33756033	5	34	theme	individual	707:716	arg1	clearances					724:733	individual serum clearances	707:733	individual serum clearances	707:733	Each IL-6 glycoform was tested in three animals and reproducibly showed individual serum clearances depending on the structure of the N-glycan.					
33756033	6	35	theme	asialo	896:901	arg1	IL-6					903:906	the corresponding asialo IL-6	878:906	the corresponding asialo IL-6	878:906	The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.					
33756033	7	36	theme	most	1077:1080	arg1	cases					1082:1086	most cases	1077:1086	most cases	1077:1086	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	1	37	theme	human	101:105	arg1	interleukin					107:117	human interleukin 6	101:119	human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans	101:170	A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans was generated by semisynthesis.					
33756033	1	37	theme	human	101:105	arg1	IL-6					122:125	IL-6	122:125	IL-6	122:125	A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans was generated by semisynthesis.					
33756033	4	38	dep	in	538:539	arg1	vivo					541:544	vivo	541:544	vivo	541:544	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	5	39	theme	serum	718:722	arg1	clearances					724:733	individual serum clearances	707:733	individual serum clearances	707:733	Each IL-6 glycoform was tested in three animals and reproducibly showed individual serum clearances depending on the structure of the N-glycan.					
33756033	6	40	theme	corresponding	882:894	arg1	IL-6					903:906	the corresponding asialo IL-6	878:906	the corresponding asialo IL-6	878:906	The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.					
33756033	4	41	gly	non-glycosylated	587:602	arg1	IL-6					616:619	non-glycosylated recombinant IL-6	587:619	non-glycosylated recombinant IL-6	587:619	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	1	42	theme	interleukin	107:117	arg1	glycoforms					87:96	glycoforms	87:96	glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans	87:170	A library of glycoforms of human interleukin 6 (IL-6) comprising complex and mannosidic N-glycans was generated by semisynthesis.					
33756033	3	43	gly	glycopeptide	321:332	arg2	glycopeptide					321:332	The central glycopeptide segments	309:341	The central glycopeptide segments	309:341	The central glycopeptide segments were assembled by pseudoproline-assisted Lansbury aspartylation and subsequent enzymatic elongation of complex N-glycans.					
33756033	7	44	theme	IL-6	992:995	arg1	glycoforms					997:1006	IL-6 glycoforms	992:1006	IL-6 glycoforms	992:1006	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	2	45	theme	native	252:257	arg1	ligation					268:275	sequential native chemical ligation	241:275	sequential native chemical ligation followed by two-step refolding	241:306	The three segments were connected by sequential native chemical ligation followed by two-step refolding.					
33756033	6	46	theme	clearance	783:791	arg1	rates					793:797	The clearance rates	779:797	The clearance rates	779:797	The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.					
33756033	6	46	theme	clearance	783:791	arg1	atypical					804:811	atypical	804:811	atypical	804:811	The clearance rates were atypical, since the 2,6-sialylated glycoforms of IL-6 cleared faster than the corresponding asialo IL-6 with terminal galactoses.					
33756033	7	47	theme	plasma	972:977	arg1	clearance					979:987	the plasma clearance	968:987	the plasma clearance of IL-6 glycoforms	968:1006	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	7	48	gly	glycoforms	997:1006	arg1	IL-6					992:995	IL-6 glycoforms	992:1006	IL-6 glycoforms	992:1006	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	7	49	theme	N-glycans	1064:1072	arg1	presence					1027:1034	the presence	1023:1034	the presence of larger and multibranched N-glycans in most cases	1023:1086	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	2	50	theme	sequential	241:250	arg1	ligation					268:275	sequential native chemical ligation	241:275	sequential native chemical ligation followed by two-step refolding	241:306	The three segments were connected by sequential native chemical ligation followed by two-step refolding.					
33756033	7	51	theme	larger	1039:1044	arg1	N-glycans					1064:1072	larger and multibranched N-glycans	1039:1072	larger and multibranched N-glycans	1039:1072	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	7	52	theme	non-glycosylated	946:961	arg1	IL-6					963:966	non-glycosylated IL-6	946:966	non-glycosylated IL-6	946:966	Compared to non-glycosylated IL-6 the plasma clearance of IL-6 glycoforms was delayed in the presence of larger and multibranched N-glycans in most cases.					
33756033	4	53	theme	IL-6	470:473	arg1	glycoforms					475:484	Nine IL-6 glycoforms	465:484	Nine IL-6 glycoforms	465:484	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	5	54	gly	glycoform	645:653	arg1	IL-6					640:643	Each IL-6 glycoform	635:653	Each IL-6 glycoform	635:653	Each IL-6 glycoform was tested in three animals and reproducibly showed individual serum clearances depending on the structure of the N-glycan.					
33756033	0	55	theme	Plasma	56:61	arg1	Clearance					63:71	Atypical Plasma Clearance	47:71	Atypical Plasma Clearance	47:71	Natural Glycoforms of Human Interleukin 6 Show Atypical Plasma Clearance.					
33756033	4	56	theme	in	538:539	arg1	clearance					553:561	in vivo plasma clearance	538:561	in vivo plasma clearance	538:561	Nine IL-6 glycoforms were synthesized, seven of which were evaluated for in vivo plasma clearance in rats and compared to non-glycosylated recombinant IL-6 from E. coli.					
33756033	5	57	theme	N-glycan	769:776	arg1	structure					752:760	the structure	748:760	the structure of the N-glycan	748:776	Each IL-6 glycoform was tested in three animals and reproducibly showed individual serum clearances depending on the structure of the N-glycan.					
32149324	5	0	gly	sialylated	905:914	arg1	compositions					887:898	the observed glycan compositions	867:898	the observed glycan compositions	867:898	Capillary electrophoresis was particularly useful in identifying charged glycans; over 40% of the observed glycan compositions were sialylated.					
32149324	5	0	gly	sialylated	905:914	arg1	%					862:862	over 40%	855:862	over 40% of the observed glycan compositions	855:898	Capillary electrophoresis was particularly useful in identifying charged glycans; over 40% of the observed glycan compositions were sialylated.					
32149324	7	1	theme	tailbud	1200:1206	arg1	stage					1208:1212	the late tailbud stage	1191:1212	the late tailbud stage (developmental stage 41)	1191:1237	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	7	1	theme	tailbud	1200:1206	arg1	stage					1229:1233	developmental stage 41	1215:1236	developmental stage 41	1215:1236	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	11	2	theme	tandem	1604:1609	arg1	spectrometry					1616:1627	tandem mass spectrometry	1604:1627	tandem mass spectrometry	1604:1627	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	3	3	theme	mass	572:575	arg1	tags					577:580	isobaric tandem mass tags	556:580	isobaric tandem mass tags	556:580	N-Glycans were labeled with isobaric tandem mass tags, pooled, separated by capillary electrophoresis, and characterized using tandem mass spectrometry.					
32149324	10	4	theme	lectin	1519:1524	arg1	blots					1566:1570	Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots	1502:1570	Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots	1502:1570	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	3	5	theme	isobaric	556:563	arg1	tags					577:580	isobaric tandem mass tags	556:580	isobaric tandem mass tags	556:580	N-Glycans were labeled with isobaric tandem mass tags, pooled, separated by capillary electrophoresis, and characterized using tandem mass spectrometry.					
32149324	6	6	theme	glycan	921:926	arg1	expression					928:937	The glycan expression	917:937	The glycan expression	917:937	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	6	6	theme	glycan	921:926	arg1	constant					954:961	constant	954:961	constant	954:961	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	6	6	theme	glycan	921:926	arg1	followed					1027:1034	followed	1027:1034	followed by massive reprogramming	1027:1059	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	4	7	theme	N-glycan	699:706	arg1	compositions					708:719	110 N-glycan compositions	695:719	110 N-glycan compositions that spanned four orders of magnitude in abundance	695:770	We quantified 110 N-glycan compositions that spanned four orders of magnitude in abundance.					
32149324	10	8	theme	Aleuria	1502:1508	arg1	AAL					1527:1529	AAL	1527:1529	AAL	1527:1529	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	10	8	theme	Aleuria	1502:1508	arg1	lectin					1519:1524	Aleuria aurantia lectin	1502:1524	Aleuria aurantia lectin (AAL)	1502:1530	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	8	9	theme	regulation	1265:1274	arg1	events					1276:1281	Two notable and opposing regulation events	1240:1281	Two notable and opposing regulation events	1240:1281	Two notable and opposing regulation events were detected for sialylated glycans.					
32149324	7	10	theme	phosphorylated	1135:1148	arg1	glycans					1166:1172	the paucimannosidic and phosphorylated oligomannosidic glycans	1111:1172	the paucimannosidic and phosphorylated oligomannosidic glycans	1111:1172	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	8	11	gly	sialylated	1301:1310	arg1	glycans					1312:1318	sialylated glycans	1301:1318	sialylated glycans	1301:1318	Two notable and opposing regulation events were detected for sialylated glycans.					
32149324	5	12	theme	observed	871:878	arg1	compositions					887:898	the observed glycan compositions	867:898	the observed glycan compositions	867:898	Capillary electrophoresis was particularly useful in identifying charged glycans; over 40% of the observed glycan compositions were sialylated.					
32149324	7	13	theme	paucimannosidic	1115:1129	arg1	glycans					1166:1172	the paucimannosidic and phosphorylated oligomannosidic glycans	1111:1172	the paucimannosidic and phosphorylated oligomannosidic glycans	1111:1172	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	5	14	theme	compositions	887:898	arg1	compositions					887:898	the observed glycan compositions	867:898	the observed glycan compositions	867:898	Capillary electrophoresis was particularly useful in identifying charged glycans; over 40% of the observed glycan compositions were sialylated.					
32149324	5	14	theme	compositions	887:898	arg1	%					862:862	over 40%	855:862	over 40% of the observed glycan compositions	855:898	Capillary electrophoresis was particularly useful in identifying charged glycans; over 40% of the observed glycan compositions were sialylated.					
32149324	6	15	theme	developmental	1002:1014	arg1	transition					990:999	the gastrula-neurula transition	969:999	the gastrula-neurula transition (developmental stage 13)	969:1024	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	6	15	theme	developmental	1002:1014	arg1	stage					1016:1020	developmental stage 13	1002:1023	developmental stage 13	1002:1023	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	11	16	gly	N-glycoproteins	1788:1802	arg1	N-glycoproteins					1788:1802	1023 N-glycoproteins	1783:1802	1023 N-glycoproteins at stage 41 (late tailbud stage)	1783:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	17	theme	developmental	1709:1721	arg1	stage					1723:1727	developmental stage 1	1709:1729	developmental stage 1 (fertilized egg)	1709:1746	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	17	theme	developmental	1709:1721	arg1	egg					1743:1745	fertilized egg	1732:1745	fertilized egg	1732:1745	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	9	18	theme	antigen	1340:1346	arg1	features					1348:1355	Lewis antigen features	1334:1355	Lewis antigen features	1334:1355	LacdiNAc and Lewis antigen features distinguished down-regulated sialylation from up-regulated species.					
32149324	3	19	theme	tandem	565:570	arg1	tags					577:580	isobaric tandem mass tags	556:580	isobaric tandem mass tags	556:580	N-Glycans were labeled with isobaric tandem mass tags, pooled, separated by capillary electrophoresis, and characterized using tandem mass spectrometry.					
32149324	11	20	from	sites	1774:1778	arg1	N-glycoproteins					1674:1688	350 N-glycoproteins	1670:1688	350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage)	1670:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	20	from	sites	1774:1778	arg1	N-glycoproteins					1788:1802	1023 N-glycoproteins	1783:1802	1023 N-glycoproteins at stage 41 (late tailbud stage)	1783:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	21	gly	N-glycosylation	1758:1772	arg2	sites					1774:1778	1682 N-glycosylation sites	1753:1778	1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage)	1753:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	21	gly	N-glycosylation	1758:1772	arg2	1682					1753:1756	1682	1753:1756	1682	1753:1756	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	0	22	theme	developmental	84:96	arg1	plan					98:101	the N-glycome developmental plan	70:101	the N-glycome developmental plan	70:101	Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis.					
32149324	11	23	gly	N-glycoproteins	1674:1688	arg1	N-glycoproteins					1674:1688	350 N-glycoproteins	1670:1688	350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage)	1670:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	1	24	theme	many	172:175	arg1	processes					188:196	many biological processes	172:196	many biological processes	172:196	Glycans are known to be involved in many biological processes, while little is known about the expression of N-glycans during vertebrate development.					
32149324	2	25	theme	African	507:513	arg1	frog					522:525	the African clawed frog	503:525	the African clawed frog	503:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	25	theme	African	507:513	arg1	laevis					495:500	Xenopus laevis	487:500	Xenopus laevis	487:500	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	11	26	theme	late	1817:1820	arg1	stage					1830:1834	late tailbud stage	1817:1834	late tailbud stage	1817:1834	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	26	theme	late	1817:1820	arg1	stage					1807:1811	stage 41	1807:1814	stage 41 (late tailbud stage)	1807:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	9	27	theme	up-regulated	1403:1414	arg1	species					1416:1422	up-regulated species	1403:1422	up-regulated species	1403:1422	LacdiNAc and Lewis antigen features distinguished down-regulated sialylation from up-regulated species.					
32149324	2	28	theme	first	304:308	arg1	studies					323:329	the first quantitative studies	300:329	the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog	300:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	29	theme	expression	414:423	arg1	studies					323:329	the first quantitative studies	300:329	the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog	300:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	12	30	theme	neuron	1934:1939	arg1	morphogenesis					1952:1964	neuron projection morphogenesis	1934:1964	neuron projection morphogenesis	1934:1964	Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.					
32149324	10	31	theme	europaeus	1541:1549	arg1	UEA-I					1559:1563	UEA-I	1559:1563	UEA-I	1559:1563	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	10	31	theme	europaeus	1541:1549	arg1	lectin					1551:1556	Ulex europaeus lectin	1536:1556	Ulex europaeus lectin (UEA-I)	1536:1564	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	0	32	theme	Quantitative	0:11	arg1	spectrometry					49:60	Quantitative capillary zone electrophoresis-mass spectrometry	0:60	Quantitative capillary zone electrophoresis-mass spectrometry	0:60	Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis.					
32149324	11	33	dep	stage	1703:1707	arg1	stage					1723:1727	developmental stage 1	1709:1729	developmental stage 1 (fertilized egg)	1709:1746	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	33	dep	stage	1703:1707	arg1	egg					1743:1745	fertilized egg	1732:1745	fertilized egg	1732:1745	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	34	theme	611	1641:1643	arg1	sites					1661:1665	611 N-glycosylation sites	1641:1665	611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage)	1641:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	2	35	theme	early	459:463	arg1	stages					477:482	two early development stages	455:482	two early development stages in Xenopus laevis, the African clawed frog	455:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	0	36	theme	zone	23:26	arg1	spectrometry					49:60	Quantitative capillary zone electrophoresis-mass spectrometry	0:60	Quantitative capillary zone electrophoresis-mass spectrometry	0:60	Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis.					
32149324	11	37	from	stage	1703:1707	arg1	N-glycoproteins					1674:1688	350 N-glycoproteins	1670:1688	350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage)	1670:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	37	from	stage	1703:1707	arg1	N-glycoproteins					1788:1802	1023 N-glycoproteins	1783:1802	1023 N-glycoproteins at stage 41 (late tailbud stage)	1783:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	38	theme	N-glycosylation	1758:1772	arg1	sites					1774:1778	1682 N-glycosylation sites	1753:1778	1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage)	1753:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	2	39	theme	N-glycosylated	428:441	arg1	peptides					443:450	N-glycosylated peptides	428:450	N-glycosylated peptides	428:450	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	5	40	theme	charged	838:844	arg1	glycans					846:852	charged glycans	838:852	charged glycans	838:852	Capillary electrophoresis was particularly useful in identifying charged glycans; over 40% of the observed glycan compositions were sialylated.					
32149324	2	41	theme	Xenopus	487:493	arg1	frog					522:525	the African clawed frog	503:525	the African clawed frog	503:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	41	theme	Xenopus	487:493	arg1	laevis					495:500	Xenopus laevis	487:500	Xenopus laevis	487:500	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	3	42	theme	mass	662:665	arg1	spectrometry					667:678	tandem mass spectrometry	655:678	tandem mass spectrometry	655:678	N-Glycans were labeled with isobaric tandem mass tags, pooled, separated by capillary electrophoresis, and characterized using tandem mass spectrometry.					
32149324	10	43	theme	Lewis	1438:1442	arg1	antigen					1444:1450	Lewis antigen	1438:1450	Lewis antigen	1438:1450	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	2	44	theme	glycans	366:372	arg1	expression					343:352	the expression	339:352	the expression of N-linked glycans at six early development stages	339:404	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	44	theme	glycans	366:372	arg1	expression					414:423	the expression	410:423	the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog	410:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	11	45	gly	N-glycosylation	1645:1659	arg2	sites					1661:1665	611 N-glycosylation sites	1641:1665	611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage)	1641:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	12	46	theme	late	1895:1898	arg1	stage					1908:1912	the late tailbud stage	1891:1912	the late tailbud stage	1891:1912	Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.					
32149324	10	47	theme	later	1465:1469	arg1	stages					1471:1476	later stages	1465:1476	later stages	1465:1476	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	2	48	theme	development	387:397	arg1	stages					399:404	six early development stages	377:404	six early development stages	377:404	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	4	49	from	orders	739:744	arg1	abundance					762:770	abundance	762:770	abundance	762:770	We quantified 110 N-glycan compositions that spanned four orders of magnitude in abundance.					
32149324	6	50	theme	gastrula-neurula	973:988	arg1	transition					990:999	the gastrula-neurula transition	969:999	the gastrula-neurula transition (developmental stage 13)	969:1024	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	6	50	theme	gastrula-neurula	973:988	arg1	stage					1016:1020	developmental stage 13	1002:1023	developmental stage 13	1002:1023	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	12	51	gly	N-glycoproteins	1861:1875	arg1	N-glycoproteins					1861:1875	the N-glycoproteins	1857:1875	the N-glycoproteins identified in the late tailbud stage	1857:1912	Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.					
32149324	7	52	theme	late	1195:1198	arg1	stage					1208:1212	the late tailbud stage	1191:1212	the late tailbud stage (developmental stage 41)	1191:1237	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	7	52	theme	late	1195:1198	arg1	stage					1229:1233	developmental stage 41	1215:1236	developmental stage 41	1215:1236	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	6	53	dep	constant	954:961	arg1	expression					928:937	The glycan expression	917:937	The glycan expression	917:937	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	6	53	dep	constant	954:961	arg1	constant					954:961	constant	954:961	constant	954:961	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	6	53	dep	constant	954:961	arg1	followed					1027:1034	followed	1027:1034	followed by massive reprogramming	1027:1059	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	2	54	link	N-linked	357:364	arg1	glycans					366:372	N-linked glycans	357:372	N-linked glycans	357:372	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	11	55	theme	mass	1611:1614	arg1	spectrometry					1616:1627	tandem mass spectrometry	1604:1627	tandem mass spectrometry	1604:1627	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	2	56	theme	quantitative	310:321	arg1	studies					323:329	the first quantitative studies	300:329	the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog	300:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	12	57	theme	neuronal	2011:2018	arg1	development					2020:2030	neuronal development	2011:2030	neuronal development	2011:2030	Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.					
32149324	8	58	theme	sialylated	1301:1310	arg1	glycans					1312:1318	sialylated glycans	1301:1318	sialylated glycans	1301:1318	Two notable and opposing regulation events were detected for sialylated glycans.					
32149324	11	59	theme	fertilized	1732:1741	arg1	stage					1723:1727	developmental stage 1	1709:1729	developmental stage 1 (fertilized egg)	1709:1746	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	59	theme	fertilized	1732:1741	arg1	egg					1743:1745	fertilized egg	1732:1745	fertilized egg	1732:1745	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	7	60	theme	oligomannosidic	1150:1164	arg1	glycans					1166:1172	the paucimannosidic and phosphorylated oligomannosidic glycans	1111:1172	the paucimannosidic and phosphorylated oligomannosidic glycans	1111:1172	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	10	61	theme	aurantia	1510:1517	arg1	AAL					1527:1529	AAL	1527:1529	AAL	1527:1529	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	10	61	theme	aurantia	1510:1517	arg1	lectin					1519:1524	Aleuria aurantia lectin	1502:1524	Aleuria aurantia lectin (AAL)	1502:1530	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	3	62	theme	pooled	583:588	arg1	tags					577:580	isobaric tandem mass tags	556:580	isobaric tandem mass tags	556:580	N-Glycans were labeled with isobaric tandem mass tags, pooled, separated by capillary electrophoresis, and characterized using tandem mass spectrometry.					
32149324	8	63	theme	notable	1244:1250	arg1	events					1276:1281	Two notable and opposing regulation events	1240:1281	Two notable and opposing regulation events	1240:1281	Two notable and opposing regulation events were detected for sialylated glycans.					
32149324	9	64	from	species	1416:1422	arg1	sialylation					1386:1396	down-regulated sialylation	1371:1396	down-regulated sialylation from up-regulated species	1371:1422	LacdiNAc and Lewis antigen features distinguished down-regulated sialylation from up-regulated species.					
32149324	11	65	used	used	1581:1584	arg2	We					1573:1574	We	1573:1574	We	1573:1574	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	7	66	from	increase	1065:1072	arg1	glycans					1166:1172	the paucimannosidic and phosphorylated oligomannosidic glycans	1111:1172	the paucimannosidic and phosphorylated oligomannosidic glycans	1111:1172	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	7	66	from	increase	1065:1072	arg1	oligomannosidic					1077:1091	oligomannosidic	1077:1091	oligomannosidic	1077:1091	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	3	67	theme	capillary	604:612	arg1	electrophoresis					614:628	capillary electrophoresis	604:628	capillary electrophoresis	604:628	N-Glycans were labeled with isobaric tandem mass tags, pooled, separated by capillary electrophoresis, and characterized using tandem mass spectrometry.					
32149324	5	68	theme	glycan	880:885	arg1	compositions					887:898	the observed glycan compositions	867:898	the observed glycan compositions	867:898	Capillary electrophoresis was particularly useful in identifying charged glycans; over 40% of the observed glycan compositions were sialylated.					
32149324	12	69	theme	N-glycome	1998:2006	arg1	role					1986:1989	a vital role	1978:1989	a vital role of the N-glycome in neuronal development	1978:2030	Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.					
32149324	11	70	from	stage	1807:1811	arg1	N-glycoproteins					1788:1802	1023 N-glycoproteins	1783:1802	1023 N-glycoproteins at stage 41 (late tailbud stage)	1783:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	7	71	located	observed	1179:1186	arg1	stage					1208:1212	the late tailbud stage	1191:1212	the late tailbud stage (developmental stage 41)	1191:1237	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	7	71	located	observed	1179:1186	arg1	stage					1229:1233	developmental stage 41	1215:1236	developmental stage 41	1215:1236	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	7	71	located	observed	1179:1186	arg2	increase					1065:1072	An increase	1062:1072	An increase in oligomannosidic	1062:1091	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	7	71	located	observed	1179:1186	arg2	decrease					1099:1106	a decrease	1097:1106	a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans	1097:1172	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	9	72	theme	Lewis	1334:1338	arg1	features					1348:1355	Lewis antigen features	1334:1355	Lewis antigen features	1334:1355	LacdiNAc and Lewis antigen features distinguished down-regulated sialylation from up-regulated species.					
32149324	12	73	from	role	1986:1989	arg1	development					2020:2030	neuronal development	2011:2030	neuronal development	2011:2030	Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.					
32149324	4	74	theme	magnitude	749:757	arg1	orders					739:744	four orders	734:744	four orders of magnitude in abundance	734:770	We quantified 110 N-glycan compositions that spanned four orders of magnitude in abundance.					
32149324	2	75	theme	clawed	515:520	arg1	frog					522:525	the African clawed frog	503:525	the African clawed frog	503:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	75	theme	clawed	515:520	arg1	laevis					495:500	Xenopus laevis	487:500	Xenopus laevis	487:500	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	8	76	theme	opposing	1256:1263	arg1	events					1276:1281	Two notable and opposing regulation events	1240:1281	Two notable and opposing regulation events	1240:1281	Two notable and opposing regulation events were detected for sialylated glycans.					
32149324	0	77	theme	vertebrate	110:119	arg1	embryogenesis					121:133	vertebrate embryogenesis	110:133	vertebrate embryogenesis	110:133	Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis.					
32149324	9	78	theme	down-regulated	1371:1384	arg1	sialylation					1386:1396	down-regulated sialylation	1371:1396	down-regulated sialylation from up-regulated species	1371:1422	LacdiNAc and Lewis antigen features distinguished down-regulated sialylation from up-regulated species.					
32149324	1	79	theme	biological	177:186	arg1	processes					188:196	many biological processes	172:196	many biological processes	172:196	Glycans are known to be involved in many biological processes, while little is known about the expression of N-glycans during vertebrate development.					
32149324	2	80	from	studies	323:329	arg1	stages					399:404	six early development stages	377:404	six early development stages	377:404	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	80	from	studies	323:329	arg1	stages					477:482	two early development stages	455:482	two early development stages in Xenopus laevis, the African clawed frog	455:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	81	from	stages	477:482	arg1	expression					343:352	the expression	339:352	the expression of N-linked glycans at six early development stages	339:404	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	81	from	stages	477:482	arg1	expression					414:423	the expression	410:423	the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog	410:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	81	from	stages	477:482	arg1	laevis					495:500	Xenopus laevis	487:500	Xenopus laevis	487:500	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	81	from	stages	477:482	arg1	studies					323:329	the first quantitative studies	300:329	the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog	300:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	81	from	stages	477:482	arg1	frog					522:525	the African clawed frog	503:525	the African clawed frog	503:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	12	82	theme	vital	1980:1984	arg1	role					1986:1989	a vital role	1978:1989	a vital role of the N-glycome in neuronal development	1978:2030	Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.					
32149324	6	83	theme	massive	1039:1045	arg1	reprogramming					1047:1059	massive reprogramming	1039:1059	massive reprogramming	1039:1059	The glycan expression was relatively constant until the gastrula-neurula transition (developmental stage 13), followed by massive reprogramming.					
32149324	2	84	gly	N-glycosylated	428:441	arg1	peptides					443:450	N-glycosylated peptides	428:450	N-glycosylated peptides	428:450	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	11	85	theme	tailbud	1822:1828	arg1	stage					1830:1834	late tailbud stage	1817:1834	late tailbud stage	1817:1834	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	11	85	theme	tailbud	1822:1828	arg1	stage					1807:1811	stage 41	1807:1814	stage 41 (late tailbud stage)	1807:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	5	86	theme	Capillary	773:781	arg1	electrophoresis					783:797	Capillary electrophoresis	773:797	Capillary electrophoresis	773:797	Capillary electrophoresis was particularly useful in identifying charged glycans; over 40% of the observed glycan compositions were sialylated.					
32149324	12	87	theme	projection	1941:1950	arg1	morphogenesis					1952:1964	neuron projection morphogenesis	1934:1964	neuron projection morphogenesis	1934:1964	Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.					
32149324	11	88	from	sites	1661:1665	arg1	N-glycoproteins					1674:1688	350 N-glycoproteins	1670:1688	350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage)	1670:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	10	89	theme	lectin	1551:1556	arg1	blots					1566:1570	Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots	1502:1570	Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots	1502:1570	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	0	90	theme	capillary	13:21	arg1	spectrometry					49:60	Quantitative capillary zone electrophoresis-mass spectrometry	0:60	Quantitative capillary zone electrophoresis-mass spectrometry	0:60	Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis.					
32149324	2	91	theme	development	465:475	arg1	stages					477:482	two early development stages	455:482	two early development stages in Xenopus laevis, the African clawed frog	455:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	10	92	theme	Ulex	1536:1539	arg1	UEA-I					1559:1563	UEA-I	1559:1563	UEA-I	1559:1563	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	10	92	theme	Ulex	1536:1539	arg1	lectin					1551:1556	Ulex europaeus lectin	1536:1556	Ulex europaeus lectin (UEA-I)	1536:1564	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	0	93	theme	electrophoresis-mass	28:47	arg1	spectrometry					49:60	Quantitative capillary zone electrophoresis-mass spectrometry	0:60	Quantitative capillary zone electrophoresis-mass spectrometry	0:60	Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis.					
32149324	11	94	theme	early	1697:1701	arg1	stage					1703:1707	the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage)	1693:1835	stage	1703:1707	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	2	95	theme	peptides	443:450	arg1	expression					343:352	the expression	339:352	the expression of N-linked glycans at six early development stages	339:404	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	95	theme	peptides	443:450	arg1	expression					414:423	the expression	410:423	the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog	410:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	7	96	theme	developmental	1215:1227	arg1	stage					1208:1212	the late tailbud stage	1191:1212	the late tailbud stage (developmental stage 41)	1191:1237	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	7	96	theme	developmental	1215:1227	arg1	stage					1229:1233	developmental stage 41	1215:1236	developmental stage 41	1215:1236	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	3	97	theme	tandem	655:660	arg1	spectrometry					667:678	tandem mass spectrometry	655:678	tandem mass spectrometry	655:678	N-Glycans were labeled with isobaric tandem mass tags, pooled, separated by capillary electrophoresis, and characterized using tandem mass spectrometry.					
32149324	10	98	theme	antigen	1444:1450	arg1	level					1429:1433	The level	1425:1433	The level of Lewis antigen	1425:1450	The level of Lewis antigen decreased at later stages, which was validated by Aleuria aurantia lectin (AAL) and Ulex europaeus lectin (UEA-I) blots.					
32149324	0	99	theme	N-glycome	74:82	arg1	plan					98:101	the N-glycome developmental plan	70:101	the N-glycome developmental plan	70:101	Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis.					
32149324	2	100	from	stages	399:404	arg1	expression					343:352	the expression	339:352	the expression of N-linked glycans at six early development stages	339:404	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	100	from	stages	399:404	arg1	expression					414:423	the expression	410:423	the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog	410:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	2	100	from	stages	399:404	arg1	studies					323:329	the first quantitative studies	300:329	the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog	300:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	11	101	theme	N-glycosylation	1645:1659	arg1	sites					1661:1665	611 N-glycosylation sites	1641:1665	611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage)	1641:1835	We also used HPLC coupled with tandem mass spectrometry to identify 611 N-glycosylation sites on 350 N-glycoproteins at the early stage developmental stage 1 (fertilized egg), and 1682 N-glycosylation sites on 1023 N-glycoproteins at stage 41 (late tailbud stage).					
32149324	2	102	theme	N-linked	357:364	arg1	glycans					366:372	N-linked glycans	357:372	N-linked glycans	357:372	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	12	103	theme	tailbud	1900:1906	arg1	stage					1908:1912	the late tailbud stage	1891:1912	the late tailbud stage	1891:1912	Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.					
32149324	12	104	theme	N-glycoproteins	1861:1875	arg1	thirds					1847:1852	Over two thirds	1838:1852	Over two thirds of the N-glycoproteins identified in the late tailbud stage	1838:1912	Over two thirds of the N-glycoproteins identified in the late tailbud stage are associated with neuron projection morphogenesis, suggesting a vital role of the N-glycome in neuronal development.					
32149324	2	105	theme	expression	343:352	arg1	studies					323:329	the first quantitative studies	300:329	the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog	300:525	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	1	106	theme	N-glycans	245:253	arg1	expression					231:240	the expression	227:240	the expression of N-glycans during vertebrate development	227:283	Glycans are known to be involved in many biological processes, while little is known about the expression of N-glycans during vertebrate development.					
32149324	7	107	from	decrease	1099:1106	arg1	glycans					1166:1172	the paucimannosidic and phosphorylated oligomannosidic glycans	1111:1172	the paucimannosidic and phosphorylated oligomannosidic glycans	1111:1172	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	7	107	from	decrease	1099:1106	arg1	oligomannosidic					1077:1091	oligomannosidic	1077:1091	oligomannosidic	1077:1091	An increase in oligomannosidic and a decrease in the paucimannosidic and phosphorylated oligomannosidic glycans were observed at the late tailbud stage (developmental stage 41).					
32149324	2	108	theme	early	381:385	arg1	stages					399:404	six early development stages	377:404	six early development stages	377:404	We now report the first quantitative studies of both the expression of N-linked glycans at six early development stages and the expression of N-glycosylated peptides at two early development stages in Xenopus laevis, the African clawed frog.					
32149324	1	109	theme	vertebrate	262:271	arg1	development					273:283	vertebrate development	262:283	vertebrate development	262:283	Glycans are known to be involved in many biological processes, while little is known about the expression of N-glycans during vertebrate development.					
34342671	0	0	theme	negative	69:76	arg1	spectra					82:88	the negative ion spectra	65:88	the negative ion spectra of neutral N-glycans from viral and other glycoproteins	65:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	0	1	from	fragmentation	14:26	arg1	spectra					82:88	the negative ion spectra	65:88	the negative ion spectra of neutral N-glycans from viral and other glycoproteins	65:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	1	2	theme	collision-induced	243:259	arg1	dissociation					261:272	negative ion collision-induced dissociation	230:272	negative ion collision-induced dissociation	230:272	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
34342671	5	3	dep	[M	713:714	arg1	addition					701:708	addition	701:708	addition	701:708	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	1	4	theme	Structural	147:156	arg1	determination					158:170	Structural determination	147:170	Structural determination of N-glycans by mass spectrometry	147:204	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
34342671	6	5	from	presence	1080:1087	arg1	spectra					1124:1130	the CID spectra	1116:1130	the CID spectra	1116:1130	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	5	6	theme	other	924:928	arg1	glycoproteins					930:942	other glycoproteins	924:942	other glycoproteins	924:942	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	1	7	theme	structural	360:369	arg1	details					371:377	structural details	360:377	structural details not available	360:391	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
34342671	5	8	dep	ions	727:730	arg1	uses					744:747	uses	744:747	uses phosphate as the adduct	744:771	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	6	9	theme	diagnostic	1092:1101	arg1	fragments					1103:1111	diagnostic fragments	1092:1111	diagnostic fragments	1092:1111	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	6	10	theme	particular	1052:1061	arg1	reference					1063:1071	particular reference	1052:1071	particular reference to the presence of diagnostic fragments in the CID spectra	1052:1130	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	6	11	theme	different	1006:1014	arg1	types					1016:1020	these different types	1000:1020	these different types of multiply charged ions	1000:1045	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	0	12	from	glycoproteins	132:144	arg1	spectra					82:88	the negative ion spectra	65:88	the negative ion spectra of neutral N-glycans from viral and other glycoproteins	65:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	0	12	from	glycoproteins	132:144	arg1	N-glycans					101:109	neutral N-glycans	93:109	neutral N-glycans from viral and other glycoproteins	93:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	0	13	theme	ion	78:80	arg1	spectra					82:88	the negative ion spectra	65:88	the negative ion spectra of neutral N-glycans from viral and other glycoproteins	65:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	6	14	dep	formation	969:977	arg1	the					965:967	the	965:967	the	965:967	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	0	15	theme	neutral	93:99	arg1	N-glycans					101:109	neutral N-glycans	93:109	neutral N-glycans from viral and other glycoproteins	93:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	5	16	theme	n	851:851	arg1	n-					853:854	(H2PO4)n]n-	844:854	(H2PO4)n]n-	844:854	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	4	17	theme	ions	628:631	arg1	fragmentation					585:597	the fragmentation	581:597	the fragmentation of singly and doubly charged ions	581:631	Differences have been reported between the fragmentation of singly and doubly charged ions but a detailed comparison does not appear to have been reported.					
34342671	2	18	theme	larger	469:474	arg1	ones					476:479	larger ones	469:479	larger ones (above approx	469:493	Most glycans form [M - H]- or [M + adduct]- ions but larger ones (above approx.					
34342671	1	19	theme	available	383:391	arg1	details					371:377	structural details	360:377	structural details not available	360:391	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
34342671	5	20	located	observed	866:873	arg1	mixtures					878:885	mixtures	878:885	mixtures of N-glycans released from viral and other glycoproteins	878:942	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	5	20	located	observed	866:873	arg2	ions					818:821	other doubly, triply, and quadruply charged ions	774:821	other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n-	774:854	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	6	21	theme	charged	1034:1040	arg1	ions					1042:1045	multiply charged ions	1025:1045	multiply charged ions	1025:1045	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	6	22	theme	CID	1120:1122	arg1	spectra					1124:1130	the CID spectra	1116:1130	the CID spectra	1116:1130	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	5	23	theme	composition	826:836	arg1	[Mn					838:840	composition [Mn	826:840	composition [Mn	826:840	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	1	24	theme	N-glycans	175:183	arg1	determination					158:170	Structural determination	147:170	Structural determination of N-glycans by mass spectrometry	147:204	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
34342671	5	25	theme	[Mn	838:840	arg1	ions					818:821	other doubly, triply, and quadruply charged ions	774:821	other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n-	774:854	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	4	26	theme	charged	620:626	arg1	ions					628:631	singly and doubly charged ions	602:631	singly and doubly charged ions	602:631	Differences have been reported between the fragmentation of singly and doubly charged ions but a detailed comparison does not appear to have been reported.					
34342671	2	27	theme	H	439:439	arg1	ions					460:463	H]- or [M + adduct]- ions	439:463	H]- or [M + adduct]- ions	439:463	Most glycans form [M - H]- or [M + adduct]- ions but larger ones (above approx.					
34342671	6	28	attach	presence	1080:1087	arg1	spectra					1124:1130	the CID spectra	1116:1130	the CID spectra	1116:1130	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	6	28	attach	presence	1080:1087	arg2	fragments					1103:1111	diagnostic fragments	1092:1111	diagnostic fragments	1092:1111	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	2	29	dep	ones	476:479	arg1	above					482:486	above	482:486	above	482:486	Most glycans form [M - H]- or [M + adduct]- ions but larger ones (above approx.					
34342671	6	30	theme	types	1016:1020	arg1	fragmentation					983:995	fragmentation	983:995	fragmentation	983:995	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	6	30	theme	types	1016:1020	arg1	formation					969:977	formation	969:977	formation	969:977	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	1	31	theme	mass	188:191	arg1	spectrometry					193:204	mass spectrometry	188:204	mass spectrometry	188:204	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
34342671	0	32	theme	N-glycans	101:109	arg1	spectra					82:88	the negative ion spectra	65:88	the negative ion spectra of neutral N-glycans from viral and other glycoproteins	65:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	1	33	theme	many	396:399	arg1	methods					407:413	many other methods	396:413	many other methods	396:413	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
34342671	4	34	theme	detailed	639:646	arg1	comparison					648:657	a detailed comparison	637:657	a detailed comparison	637:657	Differences have been reported between the fragmentation of singly and doubly charged ions but a detailed comparison does not appear to have been reported.					
34342671	2	35	theme	[M	446:447	arg1	ions					460:463	H]- or [M + adduct]- ions	439:463	H]- or [M + adduct]- ions	439:463	Most glycans form [M - H]- or [M + adduct]- ions but larger ones (above approx.					
34342671	5	36	attach	released	900:907	arg2	N-glycans					890:898	N-glycans	890:898	N-glycans released from viral and other glycoproteins	890:942	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	5	36	attach	released	900:907	arg1	glycoproteins					930:942	other glycoproteins	924:942	other glycoproteins	924:942	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	5	36	attach	released	900:907	arg1	viral					914:918	viral	914:918	viral	914:918	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	6	37	used	used	1170:1173	arg2	ions					1158:1161	these ions	1152:1161	these ions	1152:1161	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	2	38	dep	above	482:486	arg1	approx					488:493	approx	488:493	approx	488:493	Most glycans form [M - H]- or [M + adduct]- ions but larger ones (above approx.					
34342671	1	39	theme	other	401:405	arg1	methods					407:413	many other methods	396:413	many other methods	396:413	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
34342671	5	40	dep	other	774:778	arg1	charged					810:816	charged	810:816	charged	810:816	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	1	41	theme	cross-ring	311:320	arg1	fragments					322:330	cross-ring fragments	311:330	cross-ring fragments leading to ions that reveal structural details not available by many other methods	311:413	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
34342671	5	42	theme	other	774:778	arg1	ions					818:821	other doubly, triply, and quadruply charged ions	774:821	other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n-	774:854	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	6	43	theme	fragments	1103:1111	arg1	presence					1080:1087	the presence	1076:1087	the presence of diagnostic fragments in the CID spectra	1076:1130	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	2	44	theme	Most	416:419	arg1	glycans					421:427	Most glycans	416:427	Most glycans	416:427	Most glycans form [M - H]- or [M + adduct]- ions but larger ones (above approx.					
34342671	0	45	theme	charged	49:55	arg1	ions					57:60	doubly and triply charged ions	31:60	doubly and triply charged ions	31:60	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	3	46	theme	charged	528:534	arg1	ions					536:539	doubly charged ions	521:539	doubly charged ions	521:539	m/z 2000) typically form doubly charged ions.					
34342671	0	47	from	Formation	0:8	arg1	spectra					82:88	the negative ion spectra	65:88	the negative ion spectra of neutral N-glycans from viral and other glycoproteins	65:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	0	48	theme	other	126:130	arg1	glycoproteins					132:144	other glycoproteins	126:144	other glycoproteins	126:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	5	49	theme	N-glycans	890:898	arg1	mixtures					878:885	mixtures	878:885	mixtures of N-glycans released from viral and other glycoproteins	878:942	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	5	50	gly	glycoproteins	930:942	arg1	glycoproteins					930:942	other glycoproteins	924:942	other glycoproteins	924:942	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	5	51	theme	n-	853:854	arg1	ions					818:821	other doubly, triply, and quadruply charged ions	774:821	other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n-	774:854	In addition to [M + adduct]- ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [Mn + (H2PO4)n]n- have been observed in mixtures of N-glycans released from viral and other glycoproteins.					
34342671	0	52	from	spectra	82:88	arg1	viral					116:120	viral	116:120	viral	116:120	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	0	52	from	spectra	82:88	arg1	glycoproteins					132:144	other glycoproteins	126:144	other glycoproteins	126:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	0	53	from	viral	116:120	arg1	spectra					82:88	the negative ion spectra	65:88	the negative ion spectra of neutral N-glycans from viral and other glycoproteins	65:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	0	53	from	viral	116:120	arg1	N-glycans					101:109	neutral N-glycans	93:109	neutral N-glycans from viral and other glycoproteins	93:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	6	54	theme	ions	1042:1045	arg1	types					1016:1020	these different types	1000:1020	these different types of multiply charged ions	1000:1045	This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans.					
34342671	1	55	theme	negative	230:237	arg1	dissociation					261:272	negative ion collision-induced dissociation	230:272	negative ion collision-induced dissociation	230:272	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
34342671	0	56	theme	ions	57:60	arg1	fragmentation					14:26	fragmentation	14:26	fragmentation	14:26	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	0	56	theme	ions	57:60	arg1	Formation					0:8	Formation	0:8	Formation	0:8	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	0	57	gly	glycoproteins	132:144	arg1	glycoproteins					132:144	other glycoproteins	126:144	other glycoproteins	126:144	Formation and fragmentation of doubly and triply charged ions in the negative ion spectra of neutral N-glycans from viral and other glycoproteins.					
34342671	1	58	theme	ion	239:241	arg1	dissociation					261:272	negative ion collision-induced dissociation	230:272	negative ion collision-induced dissociation	230:272	Structural determination of N-glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods.					
31949166	3	0	theme	O-mannosyl	313:322	arg1	glycan					324:329	The core M3 O-mannosyl glycan	301:329	The core M3 O-mannosyl glycan	301:329	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	4	1	from	unit	608:611	arg1	glycan					628:633	the core M3 glycan	616:633	the core M3 glycan	616:633	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	3	2	contain	contains	331:338	arg2	RboP					366:369	RboP	366:369	RboP	366:369	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	3	2	contain	contains	331:338	arg2	feature					390:396	a characteristic feature	373:396	a characteristic feature first found in mammals	373:419	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	3	2	contain	contains	331:338	arg1	glycan					324:329	The core M3 O-mannosyl glycan	301:329	The core M3 O-mannosyl glycan	301:329	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	3	2	contain	contains	331:338	arg2	ribitol-phosphate					347:363	tandem ribitol-phosphate	340:363	tandem ribitol-phosphate (RboP)	340:370	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	5	3	dep	donor	708:712	arg1	substrates					773:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	Here, we report a series of crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates.					
31949166	0	4	theme	ribitol-phosphate	56:72	arg1	transferase					74:84	a ribitol-phosphate transferase	54:84	a ribitol-phosphate transferase related to muscular dystrophy	54:114	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	0	4	theme	ribitol-phosphate	56:72	arg1	structures					8:17	Crystal structures	0:17	Crystal structures of fukutin-related protein (FKRP)	0:51	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	1	5	theme	highly-glycosylated	144:162	arg1	protein					181:187	a highly-glycosylated surface membrane protein	142:187	a highly-glycosylated surface membrane protein	142:187	α-Dystroglycan (α-DG) is a highly-glycosylated surface membrane protein.					
31949166	1	5	theme	highly-glycosylated	144:162	arg1	α-Dystroglycan					117:130	α-Dystroglycan	117:130	α-Dystroglycan (α-DG)	117:137	α-Dystroglycan (α-DG) is a highly-glycosylated surface membrane protein.					
31949166	7	6	theme	phosphate	1011:1019	arg1	group					1021:1025	a phosphate group	1009:1025	a phosphate group on O-mannose	1009:1038	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	2	7	theme	dystrophies	288:298	arg1	dystrophies					288:298	congenital muscular dystrophies	268:298	congenital muscular dystrophies	268:298	Defects in the O-mannosyl glycan of α-DG cause dystroglycanopathy, a group of congenital muscular dystrophies.					
31949166	2	7	theme	dystrophies	288:298	arg1	dystroglycanopathy					237:254	dystroglycanopathy	237:254	dystroglycanopathy	237:254	Defects in the O-mannosyl glycan of α-DG cause dystroglycanopathy, a group of congenital muscular dystrophies.					
31949166	2	7	theme	dystrophies	288:298	arg1	group					259:263	a group	257:263	a group of congenital muscular dystrophies	257:298	Defects in the O-mannosyl glycan of α-DG cause dystroglycanopathy, a group of congenital muscular dystrophies.					
31949166	4	8	theme	tandem	596:601	arg1	unit					608:611	a tandem RboP unit	594:611	a tandem RboP unit in the core M3 glycan	594:633	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	0	9	theme	related	86:92	arg1	transferase					74:84	a ribitol-phosphate transferase	54:84	a ribitol-phosphate transferase related to muscular dystrophy	54:114	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	0	9	theme	related	86:92	arg1	structures					8:17	Crystal structures	0:17	Crystal structures of fukutin-related protein (FKRP)	0:51	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	6	10	from	tetramer	856:863	arg1	crystal					873:879	crystal	873:879	crystal	873:879	FKRP has N-terminal stem and C-terminal catalytic domains, and forms a tetramer both in crystal and in solution.					
31949166	6	10	from	tetramer	856:863	arg1	solution					888:895	solution	888:895	solution	888:895	FKRP has N-terminal stem and C-terminal catalytic domains, and forms a tetramer both in crystal and in solution.					
31949166	2	11	theme	muscular	279:286	arg1	dystrophies					288:298	congenital muscular dystrophies	268:298	congenital muscular dystrophies	268:298	Defects in the O-mannosyl glycan of α-DG cause dystroglycanopathy, a group of congenital muscular dystrophies.					
31949166	7	12	theme	subunit	1084:1090	arg1	domain					1066:1071	the stem domain	1057:1071	the stem domain of another subunit	1057:1090	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	7	12	theme	subunit	1084:1090	arg1	subunit					1084:1090	another subunit	1076:1090	another subunit	1076:1090	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	2	13	theme	congenital	268:277	arg1	dystrophies					288:298	congenital muscular dystrophies	268:298	congenital muscular dystrophies	268:298	Defects in the O-mannosyl glycan of α-DG cause dystroglycanopathy, a group of congenital muscular dystrophies.					
31949166	1	14	theme	surface	164:170	arg1	protein					181:187	a highly-glycosylated surface membrane protein	142:187	a highly-glycosylated surface membrane protein	142:187	α-Dystroglycan (α-DG) is a highly-glycosylated surface membrane protein.					
31949166	1	14	theme	surface	164:170	arg1	α-Dystroglycan					117:130	α-Dystroglycan	117:130	α-Dystroglycan (α-DG)	117:137	α-Dystroglycan (α-DG) is a highly-glycosylated surface membrane protein.					
31949166	0	15	theme	muscular	97:104	arg1	dystrophy					106:114	muscular dystrophy	97:114	muscular dystrophy	97:114	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	5	16	theme	M3	761:762	arg1	substrates					773:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	Here, we report a series of crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates.					
31949166	1	17	theme	membrane	172:179	arg1	protein					181:187	a highly-glycosylated surface membrane protein	142:187	a highly-glycosylated surface membrane protein	142:187	α-Dystroglycan (α-DG) is a highly-glycosylated surface membrane protein.					
31949166	1	17	theme	membrane	172:179	arg1	α-Dystroglycan					117:130	α-Dystroglycan	117:130	α-Dystroglycan (α-DG)	117:137	α-Dystroglycan (α-DG) is a highly-glycosylated surface membrane protein.					
31949166	3	18	theme	characteristic	375:388	arg1	feature					390:396	a characteristic feature	373:396	a characteristic feature first found in mammals	373:419	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	3	18	theme	characteristic	375:388	arg1	ribitol-phosphate					347:363	tandem ribitol-phosphate	340:363	tandem ribitol-phosphate (RboP)	340:370	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	4	19	theme	mutated	472:478	arg1	genes					480:484	genes	480:484	genes	480:484	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	7	20	theme	subunit	996:1002	arg1	domain					982:987	the catalytic domain	968:987	the catalytic domain of one subunit	968:1002	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	7	20	theme	subunit	996:1002	arg1	subunit					996:1002	one subunit	992:1002	one subunit	992:1002	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	0	21	theme	Crystal	0:6	arg1	transferase					74:84	a ribitol-phosphate transferase	54:84	a ribitol-phosphate transferase related to muscular dystrophy	54:114	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	0	21	theme	Crystal	0:6	arg1	structures					8:17	Crystal structures	0:17	Crystal structures of fukutin-related protein (FKRP)	0:51	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	0	22	theme	fukutin-related	22:36	arg1	FKRP					47:50	FKRP	47:50	FKRP	47:50	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	0	22	theme	fukutin-related	22:36	arg1	protein					38:44	fukutin-related protein	22:44	fukutin-related protein (FKRP)	22:51	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	5	23	theme	structures	672:681	arg1	series					654:659	a series	652:659	a series of crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates	652:782	Here, we report a series of crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates.					
31949166	8	24	theme	functional	1109:1118	arg1	studies					1120:1126	Structure-based functional studies	1093:1126	Structure-based functional studies	1093:1126	Structure-based functional studies confirmed that the dimeric structure is essential for FKRP enzymatic activity.					
31949166	4	25	theme	M3	625:626	arg1	glycan					628:633	the core M3 glycan	616:633	the core M3 glycan	616:633	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	4	26	from	diphosphate-ribitol	556:574	arg1	RboP					537:540	RboP	537:540	RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan	537:633	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	7	27	theme	catalytic	972:980	arg1	domain					982:987	the catalytic domain	968:987	the catalytic domain of one subunit	968:1002	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	7	27	theme	catalytic	972:980	arg1	subunit					996:1002	one subunit	992:1002	one subunit	992:1002	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	6	28	theme	C-terminal	814:823	arg1	domains					835:841	C-terminal catalytic domains	814:841	C-terminal catalytic domains	814:841	FKRP has N-terminal stem and C-terminal catalytic domains, and forms a tetramer both in crystal and in solution.					
31949166	6	29	contain	has	790:792	arg1	FKRP					785:788	FKRP	785:788	FKRP	785:788	FKRP has N-terminal stem and C-terminal catalytic domains, and forms a tetramer both in crystal and in solution.					
31949166	6	29	contain	has	790:792	arg2	stem					805:808	N-terminal stem	794:808	N-terminal stem	794:808	FKRP has N-terminal stem and C-terminal catalytic domains, and forms a tetramer both in crystal and in solution.					
31949166	6	29	contain	has	790:792	arg2	domains					835:841	C-terminal catalytic domains	814:841	C-terminal catalytic domains	814:841	FKRP has N-terminal stem and C-terminal catalytic domains, and forms a tetramer both in crystal and in solution.					
31949166	4	30	theme	core	620:623	arg1	glycan					628:633	the core M3 glycan	616:633	the core M3 glycan	616:633	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	5	31	theme	[RboP-	740:745	arg1	substrates					773:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	Here, we report a series of crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates.					
31949166	5	32	theme	FKRP	686:689	arg1	structures					672:681	crystal structures	664:681	crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates	664:782	Here, we report a series of crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates.					
31949166	0	33	theme	protein	38:44	arg1	transferase					74:84	a ribitol-phosphate transferase	54:84	a ribitol-phosphate transferase related to muscular dystrophy	54:114	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	0	33	theme	protein	38:44	arg1	structures					8:17	Crystal structures	0:17	Crystal structures of fukutin-related protein (FKRP)	0:51	Crystal structures of fukutin-related protein (FKRP), a ribitol-phosphate transferase related to muscular dystrophy.					
31949166	3	34	located	found	404:408	arg2	feature					390:396	a characteristic feature	373:396	a characteristic feature first found in mammals	373:419	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	3	34	located	found	404:408	arg2	ribitol-phosphate					347:363	tandem ribitol-phosphate	340:363	tandem ribitol-phosphate (RboP)	340:370	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	3	34	located	found	404:408	arg1	mammals					413:419	mammals	413:419	mammals	413:419	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	2	35	theme	α-DG	226:229	arg1	glycan					216:221	the O-mannosyl glycan	201:221	the O-mannosyl glycan of α-DG	201:229	Defects in the O-mannosyl glycan of α-DG cause dystroglycanopathy, a group of congenital muscular dystrophies.					
31949166	5	36	theme	peptide	764:770	arg1	substrates					773:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	Here, we report a series of crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates.					
31949166	5	37	theme	phospho-	747:754	arg1	substrates					773:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	Here, we report a series of crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates.					
31949166	7	38	from	group	1021:1025	arg1	O-mannose					1030:1038	O-mannose	1030:1038	O-mannose	1030:1038	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	3	39	theme	core	305:308	arg1	glycan					324:329	The core M3 O-mannosyl glycan	301:329	The core M3 O-mannosyl glycan	301:329	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	6	40	theme	N-terminal	794:803	arg1	stem					805:808	N-terminal stem	794:808	N-terminal stem	794:808	FKRP has N-terminal stem and C-terminal catalytic domains, and forms a tetramer both in crystal and in solution.					
31949166	7	41	theme	RboP	946:949	arg1	group					937:941	the phosphate group	923:941	the phosphate group of RboP	923:949	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	7	41	theme	RboP	946:949	arg1	RboP					946:949	RboP	946:949	RboP	946:949	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	4	42	theme	cytidine	547:554	arg1	CDP-Rbo					577:583	CDP-Rbo	577:583	CDP-Rbo	577:583	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	4	42	theme	cytidine	547:554	arg1	diphosphate-ribitol					556:574	cytidine diphosphate-ribitol	547:574	cytidine diphosphate-ribitol (CDP-Rbo)	547:584	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	3	43	theme	M3	310:311	arg1	glycan					324:329	The core M3 O-mannosyl glycan	301:329	The core M3 O-mannosyl glycan	301:329	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	2	44	theme	O-mannosyl	205:214	arg1	glycan					216:221	the O-mannosyl glycan	201:221	the O-mannosyl glycan of α-DG	201:229	Defects in the O-mannosyl glycan of α-DG cause dystroglycanopathy, a group of congenital muscular dystrophies.					
31949166	7	45	theme	phosphate	927:935	arg1	group					937:941	the phosphate group	923:941	the phosphate group of RboP	923:949	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	7	45	theme	phosphate	927:935	arg1	RboP					946:949	RboP	946:949	RboP	946:949	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	6	46	theme	catalytic	825:833	arg1	domains					835:841	C-terminal catalytic domains	814:841	C-terminal catalytic domains	814:841	FKRP has N-terminal stem and C-terminal catalytic domains, and forms a tetramer both in crystal and in solution.					
31949166	7	47	theme	stem	1061:1064	arg1	domain					1066:1071	the stem domain	1057:1071	the stem domain of another subunit	1057:1090	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	7	47	theme	stem	1061:1064	arg1	subunit					1084:1090	another subunit	1076:1090	another subunit	1076:1090	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	1	48	gly	highly-glycosylated	144:162	arg1	protein					181:187	a highly-glycosylated surface membrane protein	142:187	a highly-glycosylated surface membrane protein	142:187	α-Dystroglycan (α-DG) is a highly-glycosylated surface membrane protein.					
31949166	1	48	gly	highly-glycosylated	144:162	arg1	α-Dystroglycan					117:130	α-Dystroglycan	117:130	α-Dystroglycan (α-DG)	117:137	α-Dystroglycan (α-DG) is a highly-glycosylated surface membrane protein.					
31949166	5	49	theme	crystal	664:670	arg1	structures					672:681	crystal structures	664:681	crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates	664:782	Here, we report a series of crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates.					
31949166	4	50	theme	fukutin-related	434:448	arg1	FKRP					459:462	FKRP	459:462	FKRP	459:462	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	4	50	theme	fukutin-related	434:448	arg1	protein					450:456	fukutin-related protein	434:456	fukutin-related protein (FKRP)	434:463	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	4	51	theme	RboP	603:606	arg1	unit					608:611	a tandem RboP unit	594:611	a tandem RboP unit in the core M3 glycan	594:633	Fukutin and fukutin-related protein (FKRP), whose mutated genes underlie dystroglycanopathy, sequentially transfer RboP from cytidine diphosphate-ribitol (CDP-Rbo) to form a tandem RboP unit in the core M3 glycan.					
31949166	8	52	theme	enzymatic	1187:1195	arg1	activity					1197:1204	FKRP enzymatic activity	1182:1204	FKRP enzymatic activity	1182:1204	Structure-based functional studies confirmed that the dimeric structure is essential for FKRP enzymatic activity.					
31949166	8	53	theme	FKRP	1182:1185	arg1	activity					1197:1204	FKRP enzymatic activity	1182:1204	FKRP enzymatic activity	1182:1204	Structure-based functional studies confirmed that the dimeric structure is essential for FKRP enzymatic activity.					
31949166	8	54	theme	dimeric	1147:1153	arg1	essential					1168:1176	essential	1168:1176	essential	1168:1176	Structure-based functional studies confirmed that the dimeric structure is essential for FKRP enzymatic activity.					
31949166	8	54	theme	dimeric	1147:1153	arg1	structure					1155:1163	the dimeric structure	1143:1163	the dimeric structure	1143:1163	Structure-based functional studies confirmed that the dimeric structure is essential for FKRP enzymatic activity.					
31949166	3	55	theme	tandem	340:345	arg1	feature					390:396	a characteristic feature	373:396	a characteristic feature first found in mammals	373:419	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	3	55	theme	tandem	340:345	arg1	RboP					366:369	RboP	366:369	RboP	366:369	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	3	55	theme	tandem	340:345	arg1	ribitol-phosphate					347:363	tandem ribitol-phosphate	340:363	tandem ribitol-phosphate (RboP)	340:370	The core M3 O-mannosyl glycan contains tandem ribitol-phosphate (RboP), a characteristic feature first found in mammals.					
31949166	8	56	theme	Structure-based	1093:1107	arg1	studies					1120:1126	Structure-based functional studies	1093:1126	Structure-based functional studies	1093:1126	Structure-based functional studies confirmed that the dimeric structure is essential for FKRP enzymatic activity.					
31949166	7	57	theme	acceptor	905:912	arg1	complex					914:920	the acceptor complex	901:920	the acceptor complex	901:920	In the acceptor complex, the phosphate group of RboP is recognized by the catalytic domain of one subunit, and a phosphate group on O-mannose is recognized by the stem domain of another subunit.					
31949166	5	58	theme	core	756:759	arg1	substrates					773:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	[RboP-(phospho-)core M3 peptide] substrates	740:782	Here, we report a series of crystal structures of FKRP with and without donor (CDP-Rbo) and/or acceptor [RboP-(phospho-)core M3 peptide] substrates.					
31949166	2	59	from	Defects	190:196	arg1	glycan					216:221	the O-mannosyl glycan	201:221	the O-mannosyl glycan of α-DG	201:229	Defects in the O-mannosyl glycan of α-DG cause dystroglycanopathy, a group of congenital muscular dystrophies.					
32501643	0	0	theme	L5	71:72	arg1	lipoprotein					86:96	L5 low-density lipoprotein	71:96	L5 low-density lipoprotein	71:96	Increased APOE glycosylation plays a key role in the atherogenicity of L5 low-density lipoprotein.					
32501643	8	1	gly	deglycosylation	957:971	arg1	cell					988:991	endothelial cell apoptosis	976:1001	endothelial cell apoptosis	976:1001	We also examined the effects of L5 deglycosylation on endothelial cell apoptosis.					
32501643	12	2	gly	glycosylation	1790:1802	arg1	L5					1844:1845	L5	1844:1845	L5	1844:1845	APOE glycosylation may contribute to the atherogenicity of L5 and be a useful biomarker for rapidly quantifying L5.					
32501643	10	3	dep	transportation	1521:1534	arg1	functions					1551:1559	functions	1551:1559	functions	1551:1559	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	11	4	theme	glycosylation	1722:1734	arg1	removal					1736:1742	glycosylation removal	1722:1742	glycosylation removal from L5	1722:1750	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	10	5	theme	lipid-binding	1488:1500	arg1	ability					1502:1508	lipid-binding ability	1488:1508	lipid-binding ability	1488:1508	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	5	6	theme	two-dimensional	571:585	arg1	electrophoresis					587:601	two-dimensional electrophoresis	571:601	two-dimensional electrophoresis	571:601	Using two-dimensional electrophoresis and liquid chromatography-mass spectrometry, we studied APOE's posttranslational modification in L5 from human plasma.					
32501643	11	7	from	apoptosis	1615:1623	arg1	cells					1649:1653	cultured endothelial cells	1628:1653	cultured endothelial cells	1628:1653	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	12	8	theme	APOE	1785:1788	arg1	glycosylation					1790:1802	APOE glycosylation	1785:1802	APOE glycosylation	1785:1802	APOE glycosylation may contribute to the atherogenicity of L5 and be a useful biomarker for rapidly quantifying L5.					
32501643	0	9	theme	lipoprotein	86:96	arg1	atherogenicity					53:66	the atherogenicity	49:66	the atherogenicity of L5 low-density lipoprotein	49:96	Increased APOE glycosylation plays a key role in the atherogenicity of L5 low-density lipoprotein.					
32501643	12	10	theme	useful	1856:1861	arg1	biomarker					1863:1871	a useful biomarker	1854:1871	a useful biomarker for rapidly quantifying L5	1854:1898	APOE glycosylation may contribute to the atherogenicity of L5 and be a useful biomarker for rapidly quantifying L5.					
32501643	9	11	from	serine	1104:1109	arg1	L5					1159:1160	L5	1159:1160	L5	1159:1160	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	3	12	theme	E	367:367	arg1	content					376:382	Apolipoprotein E (APOE) content	352:382	Apolipoprotein E (APOE) content	352:382	Apolipoprotein E (APOE) content is increased in L5, but what role APOE plays in L5 function remains unclear.					
32501643	5	13	theme	posttranslational	666:682	arg1	modification					684:695	APOE's posttranslational modification	659:695	APOE's posttranslational modification	659:695	Using two-dimensional electrophoresis and liquid chromatography-mass spectrometry, we studied APOE's posttranslational modification in L5 from human plasma.					
32501643	11	14	theme	glycosylated	1589:1600	arg1	APOE					1602:1605	glycosylated APOE	1589:1605	glycosylated APOE	1589:1605	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	6	15	theme	APOE	722:725	arg1	structures					727:736	APOE structures	722:736	APOE structures with various glycan residues	722:765	APOE structures with various glycan residues were predicted.					
32501643	5	16	from	plasma	714:719	arg1	L5					700:701	L5	700:701	L5 from human plasma	700:719	Using two-dimensional electrophoresis and liquid chromatography-mass spectrometry, we studied APOE's posttranslational modification in L5 from human plasma.					
32501643	7	17	theme	APOE	887:890	arg1	changes					876:882	the functional changes	861:882	the functional changes of APOE resulting from glycosylation	861:919	Molecular docking and molecular dynamics simulation were performed to examine the functional changes of APOE resulting from glycosylation.					
32501643	5	18	theme	chromatography-mass	614:632	arg1	spectrometry					634:645	liquid chromatography-mass spectrometry	607:645	liquid chromatography-mass spectrometry	607:645	Using two-dimensional electrophoresis and liquid chromatography-mass spectrometry, we studied APOE's posttranslational modification in L5 from human plasma.					
32501643	5	19	theme	liquid	607:612	arg1	spectrometry					634:645	liquid chromatography-mass spectrometry	607:645	liquid chromatography-mass spectrometry	607:645	Using two-dimensional electrophoresis and liquid chromatography-mass spectrometry, we studied APOE's posttranslational modification in L5 from human plasma.					
32501643	10	20	theme	glycan	1324:1329	arg1	acids					1376:1380	positively charged amino acids	1351:1380	positively charged amino acids	1351:1380	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	20	theme	glycan	1324:1329	arg1	APOE					1342:1345	APOE	1342:1345	APOE	1342:1345	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	20	theme	glycan	1324:1329	arg1	residue					1331:1337	the negatively charged sialic acid-containing glycan residue	1278:1337	the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area	1278:1409	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	11	21	gly	glycosylated	1589:1600	arg1	APOE					1602:1605	glycosylated APOE	1589:1605	glycosylated APOE	1589:1605	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	7	22	theme	Molecular	783:791	arg1	docking					793:799	Molecular docking	783:799	Molecular docking	783:799	Molecular docking and molecular dynamics simulation were performed to examine the functional changes of APOE resulting from glycosylation.					
32501643	10	23	theme	lipid	1515:1519	arg1	transportation					1521:1534	lipid transportation	1515:1534	lipid transportation	1515:1534	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	9	24	theme	surface	1211:1217	arg1	charge					1219:1224	L5's negative surface charge	1197:1224	L5's negative surface charge	1197:1224	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	9	25	theme	glycan	1008:1013	arg1	N-acetylgalactosamine					1024:1044	The glycan sequence N-acetylgalactosamine	1004:1044	The glycan sequence N-acetylgalactosamine	1004:1044	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	10	26	theme	acid-containing	1308:1322	arg1	acids					1376:1380	positively charged amino acids	1351:1380	positively charged amino acids	1351:1380	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	26	theme	acid-containing	1308:1322	arg1	APOE					1342:1345	APOE	1342:1345	APOE	1342:1345	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	26	theme	acid-containing	1308:1322	arg1	residue					1331:1337	the negatively charged sialic acid-containing glycan residue	1278:1337	the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area	1278:1409	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	3	27	theme	role	413:416	arg1	APOE					418:421	APOE	418:421	APOE	418:421	Apolipoprotein E (APOE) content is increased in L5, but what role APOE plays in L5 function remains unclear.					
32501643	0	28	theme	APOE	10:13	arg1	glycosylation					15:27	Increased APOE glycosylation	0:27	Increased APOE glycosylation	0:27	Increased APOE glycosylation plays a key role in the atherogenicity of L5 low-density lipoprotein.					
32501643	10	29	theme	sialic	1301:1306	arg1	acids					1376:1380	positively charged amino acids	1351:1380	positively charged amino acids	1351:1380	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	29	theme	sialic	1301:1306	arg1	APOE					1342:1345	APOE	1342:1345	APOE	1342:1345	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	29	theme	sialic	1301:1306	arg1	residue					1331:1337	the negatively charged sialic acid-containing glycan residue	1278:1337	the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area	1278:1409	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	0	30	theme	Increased	0:8	arg1	glycosylation					15:27	Increased APOE glycosylation	0:27	Increased APOE glycosylation	0:27	Increased APOE glycosylation plays a key role in the atherogenicity of L5 low-density lipoprotein.					
32501643	4	31	theme	modification	528:539	arg1	contributions					488:500	the contributions	484:500	the contributions of APOE posttranslational modification to L5's atherogenicity	484:562	Here, we characterized the contributions of APOE posttranslational modification to L5's atherogenicity.					
32501643	12	32	theme	L5	1844:1845	arg1	atherogenicity					1826:1839	the atherogenicity	1822:1839	the atherogenicity of L5	1822:1845	APOE glycosylation may contribute to the atherogenicity of L5 and be a useful biomarker for rapidly quantifying L5.					
32501643	1	33	theme	variable	174:181	arg1	atherogenicity					183:196	variable atherogenicity	174:196	variable atherogenicity	174:196	Low-density lipoprotein (LDL) is heterogeneous, composed of particles with variable atherogenicity.					
32501643	9	34	theme	sequence	1015:1022	arg1	N-acetylgalactosamine					1024:1044	The glycan sequence N-acetylgalactosamine	1004:1044	The glycan sequence N-acetylgalactosamine	1004:1044	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	4	35	theme	posttranslational	510:526	arg1	modification					528:539	APOE posttranslational modification	505:539	APOE posttranslational modification	505:539	Here, we characterized the contributions of APOE posttranslational modification to L5's atherogenicity.					
32501643	5	36	theme	human	708:712	arg1	plasma					714:719	human plasma	708:719	human plasma	708:719	Using two-dimensional electrophoresis and liquid chromatography-mass spectrometry, we studied APOE's posttranslational modification in L5 from human plasma.					
32501643	7	37	theme	molecular	805:813	arg1	simulation					824:833	molecular dynamics simulation	805:833	molecular dynamics simulation	805:833	Molecular docking and molecular dynamics simulation were performed to examine the functional changes of APOE resulting from glycosylation.					
32501643	7	38	theme	functional	865:874	arg1	changes					876:882	the functional changes	861:882	the functional changes of APOE resulting from glycosylation	861:919	Molecular docking and molecular dynamics simulation were performed to examine the functional changes of APOE resulting from glycosylation.					
32501643	8	39	theme	endothelial	976:986	arg1	apoptosis					993:1001	endothelial cell apoptosis	976:1001	endothelial cell apoptosis	976:1001	We also examined the effects of L5 deglycosylation on endothelial cell apoptosis.					
32501643	7	40	theme	dynamics	815:822	arg1	simulation					824:833	molecular dynamics simulation	805:833	molecular dynamics simulation	805:833	Molecular docking and molecular dynamics simulation were performed to examine the functional changes of APOE resulting from glycosylation.					
32501643	11	41	theme	oxidized	1675:1682	arg1	LOX-1					1700:1704	LOX-1	1700:1704	LOX-1	1700:1704	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	11	41	theme	oxidized	1675:1682	arg1	receptor-1					1688:1697	lectin-like oxidized LDL receptor-1	1663:1697	lectin-like oxidized LDL receptor-1 (LOX-1) signaling	1663:1715	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	1	42	theme	Low-density	99:109	arg1	LDL					124:126	LDL	124:126	LDL	124:126	Low-density lipoprotein (LDL) is heterogeneous, composed of particles with variable atherogenicity.					
32501643	1	42	theme	Low-density	99:109	arg1	lipoprotein					111:121	Low-density lipoprotein	99:121	Low-density lipoprotein (LDL)	99:127	Low-density lipoprotein (LDL) is heterogeneous, composed of particles with variable atherogenicity.					
32501643	10	43	theme	APOE	1342:1345	arg1	acids					1376:1380	positively charged amino acids	1351:1380	positively charged amino acids	1351:1380	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	43	theme	APOE	1342:1345	arg1	APOE					1342:1345	APOE	1342:1345	APOE	1342:1345	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	43	theme	APOE	1342:1345	arg1	residue					1331:1337	the negatively charged sialic acid-containing glycan residue	1278:1337	the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area	1278:1409	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	3	44	theme	L5	432:433	arg1	function					435:442	L5 function	432:442	L5 function	432:442	Apolipoprotein E (APOE) content is increased in L5, but what role APOE plays in L5 function remains unclear.					
32501643	0	45	theme	key	37:39	arg1	role					41:44	a key role	35:44	a key role	35:44	Increased APOE glycosylation plays a key role in the atherogenicity of L5 low-density lipoprotein.					
32501643	2	46	with	patients	307:314	arg1	risk					346:349	increased cardiovascular risk	321:349	increased cardiovascular risk	321:349	Electronegative L5 LDL exhibits atherogenic properties in vitro and in vivo, and its levels are elevated in patients with increased cardiovascular risk.					
32501643	11	47	contain	containing	1578:1587	arg1	L5					1575:1576	L5	1575:1576	L5 containing glycosylated APOE	1575:1605	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	11	47	contain	containing	1578:1587	arg2	APOE					1602:1605	glycosylated APOE	1589:1605	glycosylated APOE	1589:1605	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	2	48	theme	atherogenic	231:241	arg1	properties					243:252	atherogenic properties	231:252	atherogenic properties	231:252	Electronegative L5 LDL exhibits atherogenic properties in vitro and in vivo, and its levels are elevated in patients with increased cardiovascular risk.					
32501643	8	49	theme	L5	954:955	arg1	deglycosylation					957:971	L5 deglycosylation	954:971	L5 deglycosylation	954:971	We also examined the effects of L5 deglycosylation on endothelial cell apoptosis.					
32501643	10	50	from	area	1406:1409	arg1	acids					1376:1380	positively charged amino acids	1351:1380	positively charged amino acids	1351:1380	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	50	from	area	1406:1409	arg1	APOE					1342:1345	APOE	1342:1345	APOE	1342:1345	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	50	from	area	1406:1409	arg1	residue					1331:1337	the negatively charged sialic acid-containing glycan residue	1278:1337	the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area	1278:1409	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	3	51	theme	Apolipoprotein	352:365	arg1	APOE					370:373	APOE	370:373	APOE	370:373	Apolipoprotein E (APOE) content is increased in L5, but what role APOE plays in L5 function remains unclear.					
32501643	3	51	theme	Apolipoprotein	352:365	arg1	E					367:367	Apolipoprotein E	352:367	Apolipoprotein E (APOE) content	352:382	Apolipoprotein E (APOE) content is increased in L5, but what role APOE plays in L5 function remains unclear.					
32501643	11	52	from	L5	1749:1750	arg1	removal					1736:1742	glycosylation removal	1722:1742	glycosylation removal from L5	1722:1750	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	10	53	theme	acids	1376:1380	arg1	acids					1376:1380	positively charged amino acids	1351:1380	positively charged amino acids	1351:1380	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	53	theme	acids	1376:1380	arg1	APOE					1342:1345	APOE	1342:1345	APOE	1342:1345	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	53	theme	acids	1376:1380	arg1	residue					1331:1337	the negatively charged sialic acid-containing glycan residue	1278:1337	the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area	1278:1409	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	2	54	theme	cardiovascular	331:344	arg1	risk					346:349	increased cardiovascular risk	321:349	increased cardiovascular risk	321:349	Electronegative L5 LDL exhibits atherogenic properties in vitro and in vivo, and its levels are elevated in patients with increased cardiovascular risk.					
32501643	4	55	theme	APOE	505:508	arg1	modification					528:539	APOE posttranslational modification	505:539	APOE posttranslational modification	505:539	Here, we characterized the contributions of APOE posttranslational modification to L5's atherogenicity.					
32501643	2	56	theme	L5	215:216	arg1	LDL					218:220	Electronegative L5 LDL	199:220	Electronegative L5 LDL	199:220	Electronegative L5 LDL exhibits atherogenic properties in vitro and in vivo, and its levels are elevated in patients with increased cardiovascular risk.					
32501643	9	57	from	threonine	1134:1142	arg1	L5					1159:1160	L5	1159:1160	L5	1159:1160	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	10	58	theme	amino	1370:1374	arg1	acids					1376:1380	positively charged amino acids	1351:1380	positively charged amino acids	1351:1380	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	2	59	theme	increased	321:329	arg1	risk					346:349	increased cardiovascular risk	321:349	increased cardiovascular risk	321:349	Electronegative L5 LDL exhibits atherogenic properties in vitro and in vivo, and its levels are elevated in patients with increased cardiovascular risk.					
32501643	9	60	theme	sialic	1062:1067	arg1	acid					1069:1072	sialic acid	1062:1072	sialic acid	1062:1072	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	11	61	theme	cultured	1628:1635	arg1	cells					1649:1653	cultured endothelial cells	1628:1653	cultured endothelial cells	1628:1653	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	11	62	theme	L5-induced	1763:1772	arg1	apoptosis					1774:1782	L5-induced apoptosis	1763:1782	L5-induced apoptosis	1763:1782	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	2	63	theme	Electronegative	199:213	arg1	LDL					218:220	Electronegative L5 LDL	199:220	Electronegative L5 LDL	199:220	Electronegative L5 LDL exhibits atherogenic properties in vitro and in vivo, and its levels are elevated in patients with increased cardiovascular risk.					
32501643	8	64	from	effects	943:949	arg1	apoptosis					993:1001	endothelial cell apoptosis	976:1001	endothelial cell apoptosis	976:1001	We also examined the effects of L5 deglycosylation on endothelial cell apoptosis.					
32501643	8	65	theme	deglycosylation	957:971	arg1	effects					943:949	the effects	939:949	the effects of L5 deglycosylation on endothelial cell apoptosis	939:1001	We also examined the effects of L5 deglycosylation on endothelial cell apoptosis.					
32501643	10	66	theme	electrostatic	1250:1262	arg1	force					1264:1268	The electrostatic force	1246:1268	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area	1246:1409	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	1	67	with	particles	159:167	arg1	atherogenicity					183:196	variable atherogenicity	174:196	variable atherogenicity	174:196	Low-density lipoprotein (LDL) is heterogeneous, composed of particles with variable atherogenicity.					
32501643	6	68	with	structures	727:736	arg1	residues					758:765	various glycan residues	743:765	various glycan residues	743:765	APOE structures with various glycan residues were predicted.					
32501643	9	69	theme	APOE	1151:1154	arg1	threonine					1134:1142	threonine 289	1134:1146	threonine 289	1134:1146	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	9	69	theme	APOE	1151:1154	arg1	serine					1104:1109	serine 94	1104:1112	serine 94	1104:1112	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	9	69	theme	APOE	1151:1154	arg1	threonine					1115:1123	threonine 194	1115:1127	threonine 194	1115:1127	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	6	70	theme	glycan	751:756	arg1	residues					758:765	various glycan residues	743:765	various glycan residues	743:765	APOE structures with various glycan residues were predicted.					
32501643	9	71	theme	negative	1202:1209	arg1	charge					1219:1224	L5's negative surface charge	1197:1224	L5's negative surface charge	1197:1224	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	10	72	theme	charged	1362:1368	arg1	acids					1376:1380	positively charged amino acids	1351:1380	positively charged amino acids	1351:1380	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	6	73	theme	various	743:749	arg1	residues					758:765	various glycan residues	743:765	various glycan residues	743:765	APOE structures with various glycan residues were predicted.					
32501643	11	74	theme	endothelial	1637:1647	arg1	cells					1649:1653	cultured endothelial cells	1628:1653	cultured endothelial cells	1628:1653	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	11	75	theme	lectin-like	1663:1673	arg1	LOX-1					1700:1704	LOX-1	1700:1704	LOX-1	1700:1704	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	11	75	theme	lectin-like	1663:1673	arg1	receptor-1					1688:1697	lectin-like oxidized LDL receptor-1	1663:1697	lectin-like oxidized LDL receptor-1 (LOX-1) signaling	1663:1715	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	8	76	theme	cell	988:991	arg1	apoptosis					993:1001	endothelial cell apoptosis	976:1001	endothelial cell apoptosis	976:1001	We also examined the effects of L5 deglycosylation on endothelial cell apoptosis.					
32501643	11	77	theme	LDL	1684:1686	arg1	LOX-1					1700:1704	LOX-1	1700:1704	LOX-1	1700:1704	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	11	77	theme	LDL	1684:1686	arg1	receptor-1					1688:1697	lectin-like oxidized LDL receptor-1	1663:1697	lectin-like oxidized LDL receptor-1 (LOX-1) signaling	1663:1715	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	9	78	from	threonine	1115:1123	arg1	L5					1159:1160	L5	1159:1160	L5	1159:1160	The glycan sequence N-acetylgalactosamine, galactose, and sialic acid was consistently expressed on serine 94, threonine 194, and threonine 289 of APOE in L5 and was predicted to contribute to L5's negative surface charge and hydrophilicity.					
32501643	10	79	theme	receptor-binding	1389:1404	arg1	area					1406:1409	the receptor-binding area	1385:1409	the receptor-binding area	1385:1409	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	11	80	theme	receptor-1	1688:1697	arg1	signaling					1707:1715	lectin-like oxidized LDL receptor-1 (LOX-1) signaling	1663:1715	lectin-like oxidized LDL receptor-1 (LOX-1) signaling	1663:1715	Importantly, L5 containing glycosylated APOE induced apoptosis in cultured endothelial cells through lectin-like oxidized LDL receptor-1 (LOX-1) signaling, and glycosylation removal from L5 attenuated L5-induced apoptosis.					
32501643	10	81	theme	charged	1293:1299	arg1	acids					1376:1380	positively charged amino acids	1351:1380	positively charged amino acids	1351:1380	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	81	theme	charged	1293:1299	arg1	APOE					1342:1345	APOE	1342:1345	APOE	1342:1345	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	10	81	theme	charged	1293:1299	arg1	residue					1331:1337	the negatively charged sialic acid-containing glycan residue	1278:1337	the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area	1278:1409	The electrostatic force between the negatively charged sialic acid-containing glycan residue of APOE and positively charged amino acids at the receptor-binding area suggested that glycosylation interferes with APOE's attraction to receptors, lipid-binding ability, and lipid transportation and metabolism functions.					
32501643	0	82	theme	low-density	74:84	arg1	lipoprotein					86:96	L5 low-density lipoprotein	71:96	L5 low-density lipoprotein	71:96	Increased APOE glycosylation plays a key role in the atherogenicity of L5 low-density lipoprotein.					
33450956	2	0	theme	ends	444:447	arg1	RT-PCR					401:406	RT-PCR	401:406	RT-PCR	401:406	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	2	0	theme	ends	444:447	arg1	amplification					418:430	rapid amplification	412:430	rapid amplification of the cDNA ends (RACE)	412:454	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	2	0	theme	ends	444:447	arg1	analysis					391:398	EST analysis	387:398	EST analysis	387:398	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	2	1	theme	sea	335:337	arg1	EvHc5					363:367	EvHc5	363:367	EvHc5	363:367	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	2	1	theme	sea	335:337	arg1	verrucosa					352:360	the Black sea crab Eriphia verrucosa	325:360	the Black sea crab Eriphia verrucosa (EvHc5)	325:368	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	5	2	theme	site	972:975	arg1	formation					941:949	the formation	937:949	the formation of the copper active site in Domain 2	937:987	The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2.					
33450956	0	3	theme	Subunit	71:77	arg1	Structure					19:27	Structure	19:27	Structure	19:27	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	3	theme	Subunit	71:77	arg1	Analysis					46:53	Phylogenetic Analysis	33:53	Phylogenetic Analysis	33:53	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	3	theme	Subunit	71:77	arg1	Cloning					10:16	Molecular Cloning	0:16	Molecular Cloning	0:16	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	4	from	Cloning	10:16	arg1	Malacostraca					139:150	Malacostraca	139:150	Malacostraca	139:150	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	4	from	Cloning	10:16	arg1	verrucosa					117:125	the Black Sea Crustacean Eriphia verrucosa	84:125	the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)	84:151	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	2	5	theme	Black	329:333	arg1	EvHc5					363:367	EvHc5	363:367	EvHc5	363:367	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	2	5	theme	Black	329:333	arg1	verrucosa					352:360	the Black sea crab Eriphia verrucosa	325:360	the Black sea crab Eriphia verrucosa (EvHc5)	325:368	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	0	6	theme	Black	88:92	arg1	Malacostraca					139:150	Malacostraca	139:150	Malacostraca	139:150	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	6	theme	Black	88:92	arg1	verrucosa					117:125	the Black Sea Crustacean Eriphia verrucosa	84:125	the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)	84:151	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	3	7	theme	bp	590:591	arg1	frame					576:580	an open reading frame	560:580	an open reading frame	560:580	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	3	7	theme	bp	590:591	arg1	regions					548:554	a 5' and 3' untranslated regions	523:554	a 5' and 3' untranslated regions	523:554	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	4	8	theme	hepatopancreas	758:771	arg1	cells					780:784	hepatopancreas tubule cells	758:784	hepatopancreas tubule cells	758:784	The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph.					
33450956	5	9	theme	residues	908:915	arg1	presence					843:850	the presence	839:850	the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2	839:987	The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2.					
33450956	6	10	theme	subunit	1066:1072	arg1	surface					1051:1057	the surface	1047:1057	the surface of the subunit similar to Panulirus interruptus	1047:1105	The EvHc5 is O-glycosylated and the glycan is exposed on the surface of the subunit similar to Panulirus interruptus.					
33450956	4	11	dep	proteins	734:741	arg1	synthesized					743:753	synthesized	743:753	synthesized in hepatopancreas tubule cells	743:784	The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph.					
33450956	4	11	dep	proteins	734:741	arg1	secreted					790:797	secreted	790:797	secreted into the hemolymph	790:816	The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph.					
33450956	3	12	theme	EvHc5	490:494	arg1	bp					505:506	2254 bp	500:506	2254 bp	500:506	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	3	12	theme	EvHc5	490:494	arg1	cDNA					482:485	The full-length cDNA	466:485	The full-length cDNA of EvHc5	466:494	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	0	13	theme	Crustacean	98:107	arg1	Malacostraca					139:150	Malacostraca	139:150	Malacostraca	139:150	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	13	theme	Crustacean	98:107	arg1	verrucosa					117:125	the Black Sea Crustacean Eriphia verrucosa	84:125	the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)	84:151	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	14	from	Analysis	46:53	arg1	Malacostraca					139:150	Malacostraca	139:150	Malacostraca	139:150	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	14	from	Analysis	46:53	arg1	verrucosa					117:125	the Black Sea Crustacean Eriphia verrucosa	84:125	the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)	84:151	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	3	15	theme	full-length	470:480	arg1	bp					505:506	2254 bp	500:506	2254 bp	500:506	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	3	15	theme	full-length	470:480	arg1	cDNA					482:485	The full-length cDNA	466:485	The full-length cDNA of EvHc5	466:494	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	7	16	theme	hemocyanins	1178:1188	arg1	γ-type					1168:1173	γ-type	1168:1173	γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura	1168:1266	The phylogenetic analysis has shown its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura.					
33450956	7	17	theme	other	1193:1197	arg1	species					1210:1216	other crustacean species	1193:1216	other crustacean species belonging to order Decapoda, infraorder Brachyura	1193:1266	The phylogenetic analysis has shown its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura.					
33450956	0	18	theme	Sea	94:96	arg1	Malacostraca					139:150	Malacostraca	139:150	Malacostraca	139:150	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	18	theme	Sea	94:96	arg1	verrucosa					117:125	the Black Sea Crustacean Eriphia verrucosa	84:125	the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)	84:151	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	2	19	dep	analysis	391:398	arg1	approach					456:463	approach	456:463	approach	456:463	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	7	20	theme	crustacean	1199:1208	arg1	species					1210:1216	other crustacean species	1193:1216	other crustacean species belonging to order Decapoda, infraorder Brachyura	1193:1266	The phylogenetic analysis has shown its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura.					
33450956	2	21	theme	cDNA	439:442	arg1	RACE					450:453	RACE	450:453	RACE	450:453	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	2	21	theme	cDNA	439:442	arg1	ends					444:447	the cDNA ends	435:447	the cDNA ends (RACE)	435:454	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	2	22	theme	hemocyanin	299:308	arg1	subunit					310:316	hemocyanin subunit 5	299:318	hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5)	299:368	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	1	23	theme	copper-binding	170:183	arg1	Hemocyanins					154:164	Hemocyanins	154:164	Hemocyanins	154:164	Hemocyanins are copper-binding proteins that play a crucial role in the physiological processes in crustaceans.					
33450956	1	23	theme	copper-binding	170:183	arg1	proteins					185:192	copper-binding proteins	170:192	copper-binding proteins that play a crucial role in the physiological processes in crustaceans	170:263	Hemocyanins are copper-binding proteins that play a crucial role in the physiological processes in crustaceans.					
33450956	0	24	theme	Molecular	0:8	arg1	Cloning					10:16	Molecular Cloning	0:16	Molecular Cloning	0:16	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	3	25	theme	acid	637:640	arg1	residues					642:649	674 amino acid residues	627:649	674 amino acid residues	627:649	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	5	26	theme	3D	823:824	arg1	model					826:830	The 3D model	819:830	The 3D model	819:830	The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2.					
33450956	6	27	gly	O-glycosylated	1003:1016	arg1	O-glycosylated					1003:1016	O-glycosylated	1003:1016	O-glycosylated	1003:1016	The EvHc5 is O-glycosylated and the glycan is exposed on the surface of the subunit similar to Panulirus interruptus.					
33450956	6	27	gly	O-glycosylated	1003:1016	arg1	EvHc5					994:998	The EvHc5	990:998	The EvHc5	990:998	The EvHc5 is O-glycosylated and the glycan is exposed on the surface of the subunit similar to Panulirus interruptus.					
33450956	7	28	with	grouping	1154:1161	arg1	γ-type					1168:1173	γ-type	1168:1173	γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura	1168:1266	The phylogenetic analysis has shown its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura.					
33450956	6	29	theme	similar	1074:1080	arg1	subunit					1066:1072	the subunit	1062:1072	the subunit similar to Panulirus interruptus	1062:1105	The EvHc5 is O-glycosylated and the glycan is exposed on the surface of the subunit similar to Panulirus interruptus.					
33450956	2	30	theme	EST	387:389	arg1	analysis					391:398	EST analysis	387:398	EST analysis	387:398	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	0	31	theme	Eriphia	109:115	arg1	Malacostraca					139:150	Malacostraca	139:150	Malacostraca	139:150	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	31	theme	Eriphia	109:115	arg1	verrucosa					117:125	the Black Sea Crustacean Eriphia verrucosa	84:125	the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)	84:151	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	5	32	from	formation	941:949	arg1	Domain					980:985	Domain 2	980:987	Domain 2	980:987	The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2.					
33450956	7	33	theme	phylogenetic	1112:1123	arg1	analysis					1125:1132	The phylogenetic analysis	1108:1132	The phylogenetic analysis	1108:1132	The phylogenetic analysis has shown its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura.					
33450956	0	34	theme	Phylogenetic	33:44	arg1	Analysis					46:53	Phylogenetic Analysis	33:53	Phylogenetic Analysis	33:53	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	3	35	theme	amino	631:635	arg1	residues					642:649	674 amino acid residues	627:649	674 amino acid residues	627:649	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	1	36	from	processes	240:248	arg1	crustaceans					253:263	crustaceans	253:263	crustaceans	253:263	Hemocyanins are copper-binding proteins that play a crucial role in the physiological processes in crustaceans.					
33450956	5	37	theme	conserved	888:896	arg1	residues					908:915	six conserved histidine residues	884:915	six conserved histidine residues	884:915	The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2.					
33450956	3	38	theme	untranslated	535:546	arg1	regions					548:554	a 5' and 3' untranslated regions	523:554	a 5' and 3' untranslated regions	523:554	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	5	39	theme	histidine	898:906	arg1	residues					908:915	six conserved histidine residues	884:915	six conserved histidine residues	884:915	The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2.					
33450956	1	40	theme	crucial	206:212	arg1	role					214:217	a crucial role	204:217	a crucial role	204:217	Hemocyanins are copper-binding proteins that play a crucial role in the physiological processes in crustaceans.					
33450956	4	41	contain	has	664:666	arg2	peptide					689:695	an N-terminal signal peptide	668:695	an N-terminal signal peptide of 14 amino acids	668:713	The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph.					
33450956	4	41	contain	has	664:666	arg1	protein					656:662	The protein	652:662	The protein	652:662	The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph.					
33450956	5	42	theme	active	965:970	arg1	site					972:975	the copper active site	954:975	the copper active site	954:975	The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2.					
33450956	4	43	theme	acids	709:713	arg1	peptide					689:695	an N-terminal signal peptide	668:695	an N-terminal signal peptide of 14 amino acids	668:713	The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph.					
33450956	2	44	from	verrucosa	352:360	arg1	subunit					310:316	hemocyanin subunit 5	299:318	hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5)	299:368	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	7	45	dep	order	1231:1235	arg1	infraorder					1247:1256	infraorder	1247:1256	infraorder	1247:1256	The phylogenetic analysis has shown its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura.					
33450956	7	45	dep	order	1231:1235	arg1	Brachyura					1258:1266	Brachyura	1258:1266	Brachyura	1258:1266	The phylogenetic analysis has shown its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura.					
33450956	2	46	theme	rapid	412:416	arg1	amplification					418:430	rapid amplification	412:430	rapid amplification of the cDNA ends (RACE)	412:454	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	4	47	theme	tubule	773:778	arg1	cells					780:784	hepatopancreas tubule cells	758:784	hepatopancreas tubule cells	758:784	The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph.					
33450956	4	48	theme	amino	703:707	arg1	acids					709:713	14 amino acids	700:713	14 amino acids	700:713	The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph.					
33450956	5	49	theme	copper	958:963	arg1	site					972:975	the copper active site	954:975	the copper active site	954:975	The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2.					
33450956	4	50	theme	N-terminal	671:680	arg1	peptide					689:695	an N-terminal signal peptide	668:695	an N-terminal signal peptide of 14 amino acids	668:713	The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph.					
33450956	3	51	theme	3	532:532	arg1	regions					548:554	a 5' and 3' untranslated regions	523:554	a 5' and 3' untranslated regions	523:554	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	6	52	theme	Panulirus	1085:1093	arg1	interruptus					1095:1105	Panulirus interruptus	1085:1105	Panulirus interruptus	1085:1105	The EvHc5 is O-glycosylated and the glycan is exposed on the surface of the subunit similar to Panulirus interruptus.					
33450956	3	53	theme	open	563:566	arg1	frame					576:580	an open reading frame	560:580	an open reading frame	560:580	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	5	54	theme	domains	872:878	arg1	presence					843:850	the presence	839:850	the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2	839:987	The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2.					
33450956	0	55	theme	Hemocyanin	60:69	arg1	Subunit					71:77	a Hemocyanin Subunit	58:77	a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)	58:151	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	3	56	theme	reading	568:574	arg1	frame					576:580	an open reading frame	560:580	an open reading frame	560:580	The full-length cDNA of EvHc5 was 2254 bp, consisting of a 5' and 3' untranslated regions and an open reading frame of 2022 bp, encoding a protein consisting of 674 amino acid residues.					
33450956	7	57	theme	species	1210:1216	arg1	hemocyanins					1178:1188	hemocyanins	1178:1188	hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura	1178:1266	The phylogenetic analysis has shown its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura.					
33450956	2	58	theme	Eriphia	344:350	arg1	EvHc5					363:367	EvHc5	363:367	EvHc5	363:367	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	2	58	theme	Eriphia	344:350	arg1	verrucosa					352:360	the Black sea crab Eriphia verrucosa	325:360	the Black sea crab Eriphia verrucosa (EvHc5)	325:368	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	0	59	from	verrucosa	117:125	arg1	Subunit					71:77	a Hemocyanin Subunit	58:77	a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)	58:151	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	59	from	verrucosa	117:125	arg1	Structure					19:27	Structure	19:27	Structure	19:27	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	59	from	verrucosa	117:125	arg1	Analysis					46:53	Phylogenetic Analysis	33:53	Phylogenetic Analysis	33:53	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	59	from	verrucosa	117:125	arg1	Cloning					10:16	Molecular Cloning	0:16	Molecular Cloning	0:16	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	5	60	theme	functional	861:870	arg1	domains					872:878	three functional domains	855:878	three functional domains	855:878	The 3D model showed the presence of three functional domains and six conserved histidine residues that participate in the formation of the copper active site in Domain 2.					
33450956	0	61	from	Structure	19:27	arg1	Malacostraca					139:150	Malacostraca	139:150	Malacostraca	139:150	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	0	61	from	Structure	19:27	arg1	verrucosa					117:125	the Black Sea Crustacean Eriphia verrucosa	84:125	the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)	84:151	Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca).					
33450956	4	62	theme	signal	682:687	arg1	peptide					689:695	an N-terminal signal peptide	668:695	an N-terminal signal peptide of 14 amino acids	668:713	The protein has an N-terminal signal peptide of 14 amino acids as is expected for proteins synthesized in hepatopancreas tubule cells and secreted into the hemolymph.					
33450956	2	63	theme	crab	339:342	arg1	EvHc5					363:367	EvHc5	363:367	EvHc5	363:367	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	2	63	theme	crab	339:342	arg1	verrucosa					352:360	the Black sea crab Eriphia verrucosa	325:360	the Black sea crab Eriphia verrucosa (EvHc5)	325:368	In this study, the cDNA encoding hemocyanin subunit 5 from the Black sea crab Eriphia verrucosa (EvHc5) was cloned using EST analysis, RT-PCR and rapid amplification of the cDNA ends (RACE) approach.					
33450956	7	64	theme	close	1148:1152	arg1	grouping					1154:1161	its close grouping	1144:1161	its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura	1144:1266	The phylogenetic analysis has shown its close grouping with γ-type of hemocyanins of other crustacean species belonging to order Decapoda, infraorder Brachyura.					
33450956	1	65	theme	physiological	226:238	arg1	processes					240:248	the physiological processes	222:248	the physiological processes in crustaceans	222:263	Hemocyanins are copper-binding proteins that play a crucial role in the physiological processes in crustaceans.					
34705422	5	0	theme	composited	857:866	arg1	nanofibrils					868:878	composited nanofibrils	857:878	composited nanofibrils	857:878	Fluorescence microscopic observations indicated the production of composited nanofibrils and suggested that HA secretion was associated with the cellulose secretory pathway in G. hansenii.					
34705422	0	1	theme	Engineered	90:99	arg1	Gluconacetobacter					101:117	Engineered Gluconacetobacter	90:117	Engineered Gluconacetobacter	90:117	Biofabrication of a Hyaluronan/Bacterial Cellulose Composite Nanofibril by Secretion from Engineered Gluconacetobacter.					
34705422	6	2	theme	G.	1044:1045	arg1	hansenii					1047:1054	the engineered G. hansenii	1029:1054	the engineered G. hansenii	1029:1054	The gel-like nanocomposite materials produced by the engineered G. hansenii exhibited superior properties compared with conventional in situ nanocomposites.					
34705422	3	3	theme	hyaluronan	609:618	arg1	production					595:604	simultaneous production	582:604	simultaneous production of hyaluronan (HA) and bacterial cellulose (BC)	582:652	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	6	4	theme	nanocomposite	993:1005	arg1	materials					1007:1015	The gel-like nanocomposite materials	980:1015	The gel-like nanocomposite materials produced by the engineered G. hansenii	980:1054	The gel-like nanocomposite materials produced by the engineered G. hansenii exhibited superior properties compared with conventional in situ nanocomposites.					
34705422	4	5	theme	hyaluronan	693:702	arg1	synthase					704:711	hyaluronan synthase	693:711	hyaluronan synthase	693:711	The cells were manipulated to contain hyaluronan synthase and UDP-glucose dehydrogenase genes, which are essential for HA biosynthesis.					
34705422	3	6	theme	bacterial	629:637	arg1	BC					650:651	BC	650:651	BC	650:651	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	3	6	theme	bacterial	629:637	arg1	cellulose					639:647	bacterial cellulose	629:647	bacterial cellulose (BC)	629:652	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	6	7	theme	engineered	1033:1042	arg1	hansenii					1047:1054	the engineered G. hansenii	1029:1054	the engineered G. hansenii	1029:1054	The gel-like nanocomposite materials produced by the engineered G. hansenii exhibited superior properties compared with conventional in situ nanocomposites.					
34705422	3	8	theme	cellulose	639:647	arg1	production					595:604	simultaneous production	582:604	simultaneous production of hyaluronan (HA) and bacterial cellulose (BC)	582:652	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	3	9	theme	composited	556:565	arg1	nanofibrils					567:577	naturally composited nanofibrils	546:577	naturally composited nanofibrils	546:577	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	2	10	theme	production	311:320	arg1	systems					322:328	Their hierarchically regulated production systems	280:328	Their hierarchically regulated production systems	280:328	Their hierarchically regulated production systems show potential for designing nanocomposite fabrication using engineered microorganisms.					
34705422	3	11	theme	individual	515:524	arg1	cells					526:530	genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells	451:530	genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells	451:530	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	0	12	theme	Nanofibril	61:70	arg1	Biofabrication					0:13	Biofabrication	0:13	Biofabrication of a Hyaluronan/Bacterial Cellulose Composite Nanofibril by Secretion from Engineered Gluconacetobacter.	0:118	Biofabrication of a Hyaluronan/Bacterial Cellulose Composite Nanofibril by Secretion from Engineered Gluconacetobacter.					
34705422	5	13	theme	HA	899:900	arg1	secretion					902:910	HA secretion	899:910	HA secretion	899:910	Fluorescence microscopic observations indicated the production of composited nanofibrils and suggested that HA secretion was associated with the cellulose secretory pathway in G. hansenii.					
34705422	2	14	theme	regulated	301:309	arg1	systems					322:328	Their hierarchically regulated production systems	280:328	Their hierarchically regulated production systems	280:328	Their hierarchically regulated production systems show potential for designing nanocomposite fabrication using engineered microorganisms.					
34705422	2	15	theme	nanocomposite	359:371	arg1	fabrication					373:383	nanocomposite fabrication	359:383	nanocomposite fabrication	359:383	Their hierarchically regulated production systems show potential for designing nanocomposite fabrication using engineered microorganisms.					
34705422	3	16	theme	engineered	463:472	arg1	hansenii					492:499	genetically engineered Gluconacetobacter hansenii	451:499	genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells	451:530	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	3	16	theme	engineered	463:472	arg1	G.					502:503	G.	502:503	G.	502:503	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	6	17	theme	gel-like	984:991	arg1	materials					1007:1015	The gel-like nanocomposite materials	980:1015	The gel-like nanocomposite materials produced by the engineered G. hansenii	980:1054	The gel-like nanocomposite materials produced by the engineered G. hansenii exhibited superior properties compared with conventional in situ nanocomposites.					
34705422	0	18	theme	Hyaluronan/Bacterial	20:39	arg1	Nanofibril					61:70	a Hyaluronan/Bacterial Cellulose Composite Nanofibril	18:70	a Hyaluronan/Bacterial Cellulose Composite Nanofibril	18:70	Biofabrication of a Hyaluronan/Bacterial Cellulose Composite Nanofibril by Secretion from Engineered Gluconacetobacter.					
34705422	5	19	theme	Fluorescence	791:802	arg1	observations					816:827	Fluorescence microscopic observations	791:827	Fluorescence microscopic observations	791:827	Fluorescence microscopic observations indicated the production of composited nanofibrils and suggested that HA secretion was associated with the cellulose secretory pathway in G. hansenii.					
34705422	1	20	theme	occurring	130:138	arg1	hemicellulose					176:188	hemicellulose	176:188	hemicellulose	176:188	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	1	20	theme	occurring	130:138	arg1	chitin					195:200	chitin	195:200	chitin	195:200	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	1	20	theme	occurring	130:138	arg1	cellulose					165:173	cellulose	165:173	cellulose	165:173	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	1	20	theme	occurring	130:138	arg1	polysaccharides					140:154	Naturally occurring polysaccharides	120:154	Naturally occurring polysaccharides	120:154	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	4	21	theme	UDP-glucose	717:727	arg1	dehydrogenase					729:741	UDP-glucose dehydrogenase	717:741	UDP-glucose dehydrogenase	717:741	The cells were manipulated to contain hyaluronan synthase and UDP-glucose dehydrogenase genes, which are essential for HA biosynthesis.					
34705422	5	22	theme	secretory	946:954	arg1	pathway					956:962	the cellulose secretory pathway	932:962	the cellulose secretory pathway in G. hansenii	932:977	Fluorescence microscopic observations indicated the production of composited nanofibrils and suggested that HA secretion was associated with the cellulose secretory pathway in G. hansenii.					
34705422	6	23	theme	in	1113:1114	arg1	nanocomposites					1121:1134	conventional in situ nanocomposites	1100:1134	conventional in situ nanocomposites	1100:1134	The gel-like nanocomposite materials produced by the engineered G. hansenii exhibited superior properties compared with conventional in situ nanocomposites.					
34705422	5	24	theme	microscopic	804:814	arg1	observations					816:827	Fluorescence microscopic observations	791:827	Fluorescence microscopic observations	791:827	Fluorescence microscopic observations indicated the production of composited nanofibrils and suggested that HA secretion was associated with the cellulose secretory pathway in G. hansenii.					
34705422	0	25	from	Gluconacetobacter	101:117	arg1	Secretion					75:83	Secretion	75:83	Secretion from Engineered Gluconacetobacter	75:117	Biofabrication of a Hyaluronan/Bacterial Cellulose Composite Nanofibril by Secretion from Engineered Gluconacetobacter.					
34705422	1	26	theme	plant	217:221	arg1	skeletons					223:231	plant skeletons	217:231	plant skeletons	217:231	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	0	27	theme	Composite	51:59	arg1	Nanofibril					61:70	a Hyaluronan/Bacterial Cellulose Composite Nanofibril	18:70	a Hyaluronan/Bacterial Cellulose Composite Nanofibril	18:70	Biofabrication of a Hyaluronan/Bacterial Cellulose Composite Nanofibril by Secretion from Engineered Gluconacetobacter.					
34705422	6	28	theme	conventional	1100:1111	arg1	nanocomposites					1121:1134	conventional in situ nanocomposites	1100:1134	conventional in situ nanocomposites	1100:1134	The gel-like nanocomposite materials produced by the engineered G. hansenii exhibited superior properties compared with conventional in situ nanocomposites.					
34705422	1	29	contain	have	203:206	arg1	hemicellulose					176:188	hemicellulose	176:188	hemicellulose	176:188	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	1	29	contain	have	203:206	arg1	chitin					195:200	chitin	195:200	chitin	195:200	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	1	29	contain	have	203:206	arg1	cellulose					165:173	cellulose	165:173	cellulose	165:173	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	1	29	contain	have	203:206	arg2	roles					208:212	roles	208:212	roles in plant skeletons and/or related properties in living organisms	208:277	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	1	29	contain	have	203:206	arg1	polysaccharides					140:154	Naturally occurring polysaccharides	120:154	Naturally occurring polysaccharides	120:154	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	0	30	theme	Cellulose	41:49	arg1	Nanofibril					61:70	a Hyaluronan/Bacterial Cellulose Composite Nanofibril	18:70	a Hyaluronan/Bacterial Cellulose Composite Nanofibril	18:70	Biofabrication of a Hyaluronan/Bacterial Cellulose Composite Nanofibril by Secretion from Engineered Gluconacetobacter.					
34705422	7	31	theme	integrated	1220:1229	arg1	nanomaterials					1247:1259	integrated cellulose-based nanomaterials	1220:1259	integrated cellulose-based nanomaterials	1220:1259	This genetic engineering approach facilitates the use of G. hansenii for designing integrated cellulose-based nanomaterials.					
34705422	5	32	theme	cellulose	936:944	arg1	pathway					956:962	the cellulose secretory pathway	932:962	the cellulose secretory pathway in G. hansenii	932:977	Fluorescence microscopic observations indicated the production of composited nanofibrils and suggested that HA secretion was associated with the cellulose secretory pathway in G. hansenii.					
34705422	4	33	theme	dehydrogenase	729:741	arg1	genes					743:747	hyaluronan synthase and UDP-glucose dehydrogenase genes	693:747	hyaluronan synthase and UDP-glucose dehydrogenase genes	693:747	The cells were manipulated to contain hyaluronan synthase and UDP-glucose dehydrogenase genes, which are essential for HA biosynthesis.					
34705422	3	34	theme	Gluconacetobacter	474:490	arg1	hansenii					492:499	genetically engineered Gluconacetobacter hansenii	451:499	genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells	451:530	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	3	34	theme	Gluconacetobacter	474:490	arg1	G.					502:503	G.	502:503	G.	502:503	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	3	35	theme	simultaneous	582:593	arg1	production					595:604	simultaneous production	582:604	simultaneous production of hyaluronan (HA) and bacterial cellulose (BC)	582:652	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	3	36	theme	hansenii	492:499	arg1	cells					526:530	genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells	451:530	genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells	451:530	This study has demonstrated that genetically engineered Gluconacetobacter hansenii (G. hansenii) individual cells can fabricate naturally composited nanofibrils by simultaneous production of hyaluronan (HA) and bacterial cellulose (BC).					
34705422	7	37	theme	cellulose-based	1231:1245	arg1	nanomaterials					1247:1259	integrated cellulose-based nanomaterials	1220:1259	integrated cellulose-based nanomaterials	1220:1259	This genetic engineering approach facilitates the use of G. hansenii for designing integrated cellulose-based nanomaterials.					
34705422	6	38	dep	in	1113:1114	arg1	situ					1116:1119	situ	1116:1119	situ	1116:1119	The gel-like nanocomposite materials produced by the engineered G. hansenii exhibited superior properties compared with conventional in situ nanocomposites.					
34705422	5	39	from	pathway	956:962	arg1	G.					967:968	G.	967:968	G.	967:968	Fluorescence microscopic observations indicated the production of composited nanofibrils and suggested that HA secretion was associated with the cellulose secretory pathway in G. hansenii.					
34705422	2	40	theme	engineered	391:400	arg1	microorganisms					402:415	engineered microorganisms	391:415	engineered microorganisms	391:415	Their hierarchically regulated production systems show potential for designing nanocomposite fabrication using engineered microorganisms.					
34705422	6	41	theme	superior	1066:1073	arg1	properties					1075:1084	superior properties	1066:1084	superior properties	1066:1084	The gel-like nanocomposite materials produced by the engineered G. hansenii exhibited superior properties compared with conventional in situ nanocomposites.					
34705422	1	42	theme	related	240:246	arg1	properties					248:257	related properties	240:257	related properties	240:257	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	7	43	theme	G.	1194:1195	arg1	use					1187:1189	the use	1183:1189	the use of G. hansenii for designing integrated cellulose-based nanomaterials	1183:1259	This genetic engineering approach facilitates the use of G. hansenii for designing integrated cellulose-based nanomaterials.					
34705422	7	44	theme	genetic	1142:1148	arg1	approach					1162:1169	This genetic engineering approach	1137:1169	This genetic engineering approach	1137:1169	This genetic engineering approach facilitates the use of G. hansenii for designing integrated cellulose-based nanomaterials.					
34705422	7	45	theme	engineering	1150:1160	arg1	approach					1162:1169	This genetic engineering approach	1137:1169	This genetic engineering approach	1137:1169	This genetic engineering approach facilitates the use of G. hansenii for designing integrated cellulose-based nanomaterials.					
34705422	5	46	theme	nanofibrils	868:878	arg1	production					843:852	the production	839:852	the production of composited nanofibrils	839:878	Fluorescence microscopic observations indicated the production of composited nanofibrils and suggested that HA secretion was associated with the cellulose secretory pathway in G. hansenii.					
34705422	1	47	from	roles	208:212	arg1	skeletons					223:231	plant skeletons	217:231	plant skeletons	217:231	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	1	47	from	roles	208:212	arg1	properties					248:257	related properties	240:257	related properties	240:257	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	1	47	from	roles	208:212	arg1	organisms					269:277	living organisms	262:277	living organisms	262:277	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34705422	4	48	theme	HA	774:775	arg1	biosynthesis					777:788	HA biosynthesis	774:788	HA biosynthesis	774:788	The cells were manipulated to contain hyaluronan synthase and UDP-glucose dehydrogenase genes, which are essential for HA biosynthesis.					
34705422	4	49	theme	synthase	704:711	arg1	genes					743:747	hyaluronan synthase and UDP-glucose dehydrogenase genes	693:747	hyaluronan synthase and UDP-glucose dehydrogenase genes	693:747	The cells were manipulated to contain hyaluronan synthase and UDP-glucose dehydrogenase genes, which are essential for HA biosynthesis.					
34705422	4	50	contain	contain	685:691	arg2	genes					743:747	hyaluronan synthase and UDP-glucose dehydrogenase genes	693:747	hyaluronan synthase and UDP-glucose dehydrogenase genes	693:747	The cells were manipulated to contain hyaluronan synthase and UDP-glucose dehydrogenase genes, which are essential for HA biosynthesis.					
34705422	4	50	contain	contain	685:691	arg1	cells					659:663	The cells	655:663	The cells	655:663	The cells were manipulated to contain hyaluronan synthase and UDP-glucose dehydrogenase genes, which are essential for HA biosynthesis.					
34705422	1	51	theme	living	262:267	arg1	organisms					269:277	living organisms	262:277	living organisms	262:277	Naturally occurring polysaccharides, such as cellulose, hemicellulose, and chitin, have roles in plant skeletons and/or related properties in living organisms.					
34081697	5	0	theme	cytosine	839:846	arg1	bases					848:852	its cytosine bases	835:852	its cytosine bases post-transcriptionally methylated through epigenetic processes	835:915	The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes.					
34081697	5	1	attach	presence	759:766	arg1	cells					780:784	cells	780:784	cells	780:784	The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes.					
34081697	5	1	attach	presence	759:766	arg2	m5CMP					771:775	m5CMP	771:775	m5CMP	771:775	The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes.					
34081697	4	2	theme	monophosphate	709:721	arg1	form					692:695	an endogenous methylated form	667:695	an endogenous methylated form of cytidine monophosphate (m5CMP)	667:729	Here, we describe the discovery that an endogenous methylated form of cytidine monophosphate (m5CMP) binds and inhibits CST.					
34081697	5	3	theme	epigenetic	896:905	arg1	processes					907:915	epigenetic processes	896:915	epigenetic processes	896:915	The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes.					
34081697	3	4	theme	NSTs	518:521	arg1	means					563:567	a means	561:567	a means to produce the vast diversity of observed glycan structures	561:627	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	3	4	theme	NSTs	518:521	arg1	activity					506:513	the transport activity	492:513	the transport activity of NSTs	492:521	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	3	5	theme	fundamental	452:462	arg1	question					476:483	one fundamental unaddressed question	448:483	one fundamental unaddressed question	448:483	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	1	6	theme	Nucleotide-sugar	68:83	arg1	NSTs					99:102	NSTs	99:102	NSTs	99:102	Nucleotide-sugar transporters (NSTs) transport nucleotide-sugar conjugates into the Golgi lumen where they are then used in the synthesis of glycans.					
34081697	1	6	theme	Nucleotide-sugar	68:83	arg1	transporters					85:96	Nucleotide-sugar transporters	68:96	Nucleotide-sugar transporters (NSTs)	68:103	Nucleotide-sugar transporters (NSTs) transport nucleotide-sugar conjugates into the Golgi lumen where they are then used in the synthesis of glycans.					
34081697	5	7	theme	RNA	818:820	arg1	degradation					803:813	the degradation	799:813	the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes	799:915	The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes.					
34081697	3	8	theme	selectivity	411:421	arg1	aspects					377:383	many aspects	372:383	many aspects of substrate recognition, selectivity, and transport	372:436	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	5	9	from	presence	759:766	arg1	cells					780:784	cells	780:784	cells	780:784	The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes.					
34081697	2	10	theme	mammalian	265:273	arg1	transporter					300:310	the CMP-sialic acid transporter	280:310	the CMP-sialic acid transporter (CST)	280:316	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	2	10	theme	mammalian	265:273	arg1	NST					275:277	a mammalian NST	263:277	a mammalian NST	263:277	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	6	11	theme	glycosylation	1118:1130	arg1	regulation					1104:1113	regulation	1104:1113	regulation of glycosylation	1104:1130	Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation.					
34081697	6	11	theme	glycosylation	1118:1130	arg1	control					1073:1079	epigenetic control	1062:1079	epigenetic control of gene expression	1062:1098	Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation.					
34081697	4	12	theme	cytidine	700:707	arg1	monophosphate					709:721	cytidine monophosphate	700:721	cytidine monophosphate (m5CMP)	700:729	Here, we describe the discovery that an endogenous methylated form of cytidine monophosphate (m5CMP) binds and inhibits CST.					
34081697	4	12	theme	cytidine	700:707	arg1	m5CMP					724:728	m5CMP	724:728	m5CMP	724:728	Here, we describe the discovery that an endogenous methylated form of cytidine monophosphate (m5CMP) binds and inhibits CST.					
34081697	3	13	theme	transport	496:504	arg1	means					563:567	a means	561:567	a means to produce the vast diversity of observed glycan structures	561:627	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	3	13	theme	transport	496:504	arg1	activity					506:513	the transport activity	492:513	the transport activity of NSTs	492:521	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	3	14	theme	recognition	398:408	arg1	aspects					377:383	many aspects	372:383	many aspects of substrate recognition, selectivity, and transport	372:436	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	0	15	theme	acid	25:28	arg1	transport					30:38	CMP-sialic acid transport	14:38	CMP-sialic acid transport	14:38	Inhibition of CMP-sialic acid transport by endogenous 5-methyl CMP.					
34081697	2	16	theme	acid	295:298	arg1	CST					313:315	CST	313:315	CST	313:315	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	2	16	theme	acid	295:298	arg1	transporter					300:310	the CMP-sialic acid transporter	280:310	the CMP-sialic acid transporter (CST)	280:316	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	2	16	theme	acid	295:298	arg1	NST					275:277	a mammalian NST	263:277	a mammalian NST	263:277	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	0	17	theme	CMP-sialic	14:23	arg1	transport					30:38	CMP-sialic acid transport	14:38	CMP-sialic acid transport	14:38	Inhibition of CMP-sialic acid transport by endogenous 5-methyl CMP.					
34081697	2	18	theme	NST	275:277	arg1	structures					249:258	crystal structures	241:258	crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST)	241:316	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	3	19	theme	glycan	611:616	arg1	structures					618:627	observed glycan structures	602:627	observed glycan structures	602:627	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	2	20	theme	CMP-sialic	284:293	arg1	CST					313:315	CST	313:315	CST	313:315	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	2	20	theme	CMP-sialic	284:293	arg1	transporter					300:310	the CMP-sialic acid transporter	280:310	the CMP-sialic acid transporter (CST)	280:316	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	2	20	theme	CMP-sialic	284:293	arg1	NST					275:277	a mammalian NST	263:277	a mammalian NST	263:277	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	6	21	theme	CST	1018:1020	arg1	regulator					1005:1013	a novel physiological regulator	983:1013	a novel physiological regulator of CST	983:1020	Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation.					
34081697	6	22	theme	gene	1084:1087	arg1	expression					1089:1098	gene expression	1084:1098	gene expression	1084:1098	Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation.					
34081697	3	23	theme	structures	618:627	arg1	diversity					589:597	the vast diversity	580:597	the vast diversity of observed glycan structures	580:627	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	2	24	dep	reported	232:239	arg1	Whorton					329:335	Whorton	329:335	Whorton	329:335	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	2	24	dep	reported	232:239	arg1	Ahuja					319:323	Ahuja	319:323	Ahuja	319:323	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	3	25	theme	substrate	388:396	arg1	recognition					398:408	substrate recognition	388:408	substrate recognition	388:408	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	0	26	theme	transport	30:38	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of CMP-sialic acid transport by endogenous 5-methyl CMP.	0:66	Inhibition of CMP-sialic acid transport by endogenous 5-methyl CMP.					
34081697	6	27	dep	Therefore	918:926	arg1	only					943:946	only	943:946	only	943:946	Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation.					
34081697	5	28	theme	m5CMP	771:775	arg1	presence					759:766	The presence	755:766	The presence of m5CMP in cells	755:784	The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes.					
34081697	1	29	theme	glycans	209:215	arg1	synthesis					196:204	the synthesis	192:204	the synthesis of glycans	192:215	Nucleotide-sugar transporters (NSTs) transport nucleotide-sugar conjugates into the Golgi lumen where they are then used in the synthesis of glycans.					
34081697	0	30	theme	5-methyl	54:61	arg1	CMP					63:65	endogenous 5-methyl CMP	43:65	endogenous 5-methyl CMP	43:65	Inhibition of CMP-sialic acid transport by endogenous 5-methyl CMP.					
34081697	3	31	theme	observed	602:609	arg1	structures					618:627	observed glycan structures	602:627	observed glycan structures	602:627	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	6	32	theme	epigenetic	1062:1071	arg1	control					1073:1079	epigenetic control	1062:1079	epigenetic control of gene expression	1062:1098	Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation.					
34081697	1	33	theme	nucleotide-sugar	115:130	arg1	conjugates					132:141	nucleotide-sugar conjugates	115:141	nucleotide-sugar conjugates	115:141	Nucleotide-sugar transporters (NSTs) transport nucleotide-sugar conjugates into the Golgi lumen where they are then used in the synthesis of glycans.					
34081697	0	34	theme	endogenous	43:52	arg1	CMP					63:65	endogenous 5-methyl CMP	43:65	endogenous 5-methyl CMP	43:65	Inhibition of CMP-sialic acid transport by endogenous 5-methyl CMP.					
34081697	3	35	theme	unaddressed	464:474	arg1	question					476:483	one fundamental unaddressed question	448:483	one fundamental unaddressed question	448:483	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	4	36	theme	endogenous	670:679	arg1	form					692:695	an endogenous methylated form	667:695	an endogenous methylated form of cytidine monophosphate (m5CMP)	667:729	Here, we describe the discovery that an endogenous methylated form of cytidine monophosphate (m5CMP) binds and inhibits CST.					
34081697	3	37	theme	vast	584:587	arg1	diversity					589:597	the vast diversity	580:597	the vast diversity of observed glycan structures	580:627	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	6	38	theme	physiological	991:1003	arg1	regulator					1005:1013	a novel physiological regulator	983:1013	a novel physiological regulator of CST	983:1020	Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation.					
34081697	1	39	used	used	184:187	arg2	they					170:173	they	170:173	they	170:173	Nucleotide-sugar transporters (NSTs) transport nucleotide-sugar conjugates into the Golgi lumen where they are then used in the synthesis of glycans.					
34081697	4	40	theme	methylated	681:690	arg1	form					692:695	an endogenous methylated form	667:695	an endogenous methylated form of cytidine monophosphate (m5CMP)	667:729	Here, we describe the discovery that an endogenous methylated form of cytidine monophosphate (m5CMP) binds and inhibits CST.					
34081697	6	41	theme	expression	1089:1098	arg1	regulation					1104:1113	regulation	1104:1113	regulation of glycosylation	1104:1130	Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation.					
34081697	6	41	theme	expression	1089:1098	arg1	control					1073:1079	epigenetic control	1062:1079	epigenetic control of gene expression	1062:1098	Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation.					
34081697	6	42	theme	novel	985:989	arg1	regulator					1005:1013	a novel physiological regulator	983:1013	a novel physiological regulator of CST	983:1020	Therefore, this work not only demonstrates that m5CMP represents a novel physiological regulator of CST, but it also establishes a link between epigenetic control of gene expression and regulation of glycosylation.					
34081697	3	43	theme	transport	428:436	arg1	aspects					377:383	many aspects	372:383	many aspects of substrate recognition, selectivity, and transport	372:436	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	1	44	theme	Golgi	152:156	arg1	lumen					158:162	the Golgi lumen	148:162	the Golgi lumen where they are then used in the synthesis of glycans	148:215	Nucleotide-sugar transporters (NSTs) transport nucleotide-sugar conjugates into the Golgi lumen where they are then used in the synthesis of glycans.					
34081697	3	45	theme	many	372:375	arg1	aspects					377:383	many aspects	372:383	many aspects of substrate recognition, selectivity, and transport	372:436	These structures elucidated many aspects of substrate recognition, selectivity, and transport; however, one fundamental unaddressed question is how the transport activity of NSTs might be physiologically regulated as a means to produce the vast diversity of observed glycan structures.					
34081697	2	46	theme	crystal	241:247	arg1	structures					249:258	crystal structures	241:258	crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST)	241:316	We previously reported crystal structures of a mammalian NST, the CMP-sialic acid transporter (CST) (Ahuja and Whorton 2019).					
34081697	5	47	contain	had	831:833	arg1	RNA					818:820	RNA	818:820	RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes	818:915	The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes.					
34081697	5	47	contain	had	831:833	arg2	bases					848:852	its cytosine bases	835:852	its cytosine bases post-transcriptionally methylated through epigenetic processes	835:915	The presence of m5CMP in cells results from the degradation of RNA that has had its cytosine bases post-transcriptionally methylated through epigenetic processes.					
32767150	0	0	theme	variants	79:86	arg1	patterns					23:30	N- and O-glycosylation patterns	0:30	N- and O-glycosylation patterns	0:30	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	0	0	theme	variants	79:86	arg1	testing					47:53	functional testing	36:53	functional testing	36:53	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	6	1	theme	functional	783:792	arg1	differences					794:804	possible functional differences	774:804	possible functional differences	774:804	Here, we investigate the glycan structures and possible functional differences of the two CGB variants.					
32767150	11	2	theme	functional	1433:1442	arg1	differences					1444:1454	significant functional differences	1421:1454	significant functional differences	1421:1454	In summary, the results lead to the hypothesis that CGB7 and CGB3/5/8 do not hold significant functional differences but that timing and cell type of their expression is the key for understanding their divergent evolution.					
32767150	11	3	theme	expression	1495:1504	arg1	key					1513:1515	key	1513:1515	key	1513:1515	In summary, the results lead to the hypothesis that CGB7 and CGB3/5/8 do not hold significant functional differences but that timing and cell type of their expression is the key for understanding their divergent evolution.					
32767150	11	3	theme	expression	1495:1504	arg1	timing					1465:1470	timing	1465:1470	timing	1465:1470	In summary, the results lead to the hypothesis that CGB7 and CGB3/5/8 do not hold significant functional differences but that timing and cell type of their expression is the key for understanding their divergent evolution.					
32767150	11	3	theme	expression	1495:1504	arg1	type					1481:1484	cell type	1476:1484	cell type	1476:1484	In summary, the results lead to the hypothesis that CGB7 and CGB3/5/8 do not hold significant functional differences but that timing and cell type of their expression is the key for understanding their divergent evolution.					
32767150	7	4	theme	recombinant	891:901	arg1	proteins					926:933	recombinant CGA, CGB7 and CGB3/5/8 proteins	891:933	recombinant CGA, CGB7 and CGB3/5/8 proteins	891:933	To this end, we established a system to produce and isolate recombinant CGA, CGB7 and CGB3/5/8 proteins.					
32767150	0	5	theme	CGB3/5/8	70:77	arg1	variants					79:86	CGB7 versus CGB3/5/8 variants	58:86	CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit	58:141	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	4	6	dep	code	415:418	arg1	whereas					456:462	whereas	456:462	whereas	456:462	CGB3, 5 and 8 code for an identical protein, CGB3/5/8, whereas CGB7 differs in three amino acids from CGB3/5/8.					
32767150	10	7	theme	first	1285:1289	arg1	time					1291:1294	the first time that biological activity of CGB7 is shown	1281:1336	the first time that biological activity of CGB7 is shown	1281:1336	This is the first time that biological activity of CGB7 is shown.					
32767150	10	7	theme	first	1285:1289	arg1	This					1273:1276	This	1273:1276	This	1273:1276	This is the first time that biological activity of CGB7 is shown.					
32767150	9	8	from	difference	1203:1212	arg1	kinetics					1221:1228	the kinetics	1217:1228	the kinetics of ERK1/2 pathway activation	1217:1257	Functional assays were performed by testing activation of the ERK1/2 pathway and demonstrated that CGB7 and CGB5/5/8 appear to be functionally redundant isoforms, although a slight difference in the kinetics of ERK1/2 pathway activation was observed.					
32767150	9	9	theme	Functional	1022:1031	arg1	assays					1033:1038	Functional assays	1022:1038	Functional assays	1022:1038	Functional assays were performed by testing activation of the ERK1/2 pathway and demonstrated that CGB7 and CGB5/5/8 appear to be functionally redundant isoforms, although a slight difference in the kinetics of ERK1/2 pathway activation was observed.					
32767150	8	10	theme	CGB3/5/8	994:1001	arg1	patterns					973:980	N- and O-glycosylation patterns	950:980	N- and O-glycosylation patterns of CGB7 and CGB3/5/8	950:1001	We found that N- and O-glycosylation patterns of CGB7 and CGB3/5/8 are quite similar.					
32767150	5	11	theme	distinct	573:580	arg1	patterns					600:607	very distinct tissue expression patterns	568:607	very distinct tissue expression patterns	568:607	We had observed earlier that CGB7 and CGB3/5/8 display very distinct tissue expression patterns and that the tumor suppressor and transcription factor p53 can activate expression of CGB7 but not of CGB3/5/8 genes.					
32767150	8	12	theme	N-	950:951	arg1	patterns					973:980	N- and O-glycosylation patterns	950:980	N- and O-glycosylation patterns of CGB7 and CGB3/5/8	950:1001	We found that N- and O-glycosylation patterns of CGB7 and CGB3/5/8 are quite similar.					
32767150	3	13	theme	CGB8	389:392	arg1	genes					394:398	CGB8 genes	389:398	CGB8 genes	389:398	The beta-hCG protein is encoded by CGB3, CGB5, CGB7 and CGB8 genes.					
32767150	0	14	theme	chorionic	101:109	arg1	hCG					125:127	hCG	125:127	hCG	125:127	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	0	14	theme	chorionic	101:109	arg1	gonadotropin					111:122	human chorionic gonadotropin	95:122	the human chorionic gonadotropin (hCG) beta subunit	91:141	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	6	15	theme	glycan	752:757	arg1	structures					759:768	the glycan structures	748:768	the glycan structures	748:768	Here, we investigate the glycan structures and possible functional differences of the two CGB variants.					
32767150	1	16	theme	classical	148:156	arg1	role					212:215	its role	208:215	its role in supporting pregnancy	208:239	The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy.					
32767150	1	16	theme	classical	148:156	arg1	function					158:165	The classical function	144:165	The classical function of human chorionic gonadotropin (hCG)	144:203	The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy.					
32767150	0	17	theme	human	95:99	arg1	hCG					125:127	hCG	125:127	hCG	125:127	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	0	17	theme	human	95:99	arg1	gonadotropin					111:122	human chorionic gonadotropin	95:122	the human chorionic gonadotropin (hCG) beta subunit	91:141	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	9	18	theme	redundant	1165:1173	arg1	isoforms					1175:1182	functionally redundant isoforms	1152:1182	functionally redundant isoforms	1152:1182	Functional assays were performed by testing activation of the ERK1/2 pathway and demonstrated that CGB7 and CGB5/5/8 appear to be functionally redundant isoforms, although a slight difference in the kinetics of ERK1/2 pathway activation was observed.					
32767150	3	19	theme	beta-hCG	337:344	arg1	protein					346:352	The beta-hCG protein	333:352	The beta-hCG protein	333:352	The beta-hCG protein is encoded by CGB3, CGB5, CGB7 and CGB8 genes.					
32767150	7	20	theme	CGB3/5/8	917:924	arg1	proteins					926:933	recombinant CGA, CGB7 and CGB3/5/8 proteins	891:933	recombinant CGA, CGB7 and CGB3/5/8 proteins	891:933	To this end, we established a system to produce and isolate recombinant CGA, CGB7 and CGB3/5/8 proteins.					
32767150	5	21	theme	transcription	643:655	arg1	p53					664:666	the tumor suppressor and transcription factor p53	618:666	p53	664:666	We had observed earlier that CGB7 and CGB3/5/8 display very distinct tissue expression patterns and that the tumor suppressor and transcription factor p53 can activate expression of CGB7 but not of CGB3/5/8 genes.					
32767150	10	22	theme	biological	1301:1310	arg1	activity					1312:1319	biological activity	1301:1319	biological activity of CGB7	1301:1327	This is the first time that biological activity of CGB7 is shown.					
32767150	7	23	theme	CGA	903:905	arg1	proteins					926:933	recombinant CGA, CGB7 and CGB3/5/8 proteins	891:933	recombinant CGA, CGB7 and CGB3/5/8 proteins	891:933	To this end, we established a system to produce and isolate recombinant CGA, CGB7 and CGB3/5/8 proteins.					
32767150	0	24	theme	N-	0:1	arg1	patterns					23:30	N- and O-glycosylation patterns	0:30	N- and O-glycosylation patterns	0:30	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	11	25	theme	divergent	1541:1549	arg1	evolution					1551:1559	their divergent evolution	1535:1559	their divergent evolution	1535:1559	In summary, the results lead to the hypothesis that CGB7 and CGB3/5/8 do not hold significant functional differences but that timing and cell type of their expression is the key for understanding their divergent evolution.					
32767150	1	26	theme	human	170:174	arg1	gonadotropin					186:197	human chorionic gonadotropin	170:197	human chorionic gonadotropin (hCG)	170:203	The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy.					
32767150	1	26	theme	human	170:174	arg1	hCG					200:202	hCG	200:202	hCG	200:202	The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy.					
32767150	5	27	theme	CGB7	695:698	arg1	expression					681:690	expression	681:690	expression of CGB7 but not of CGB3/5/8 genes	681:724	We had observed earlier that CGB7 and CGB3/5/8 display very distinct tissue expression patterns and that the tumor suppressor and transcription factor p53 can activate expression of CGB7 but not of CGB3/5/8 genes.					
32767150	4	28	theme	identical	427:435	arg1	protein					437:443	an identical protein	424:443	an identical protein	424:443	CGB3, 5 and 8 code for an identical protein, CGB3/5/8, whereas CGB7 differs in three amino acids from CGB3/5/8.					
32767150	4	28	theme	identical	427:435	arg1	CGB3/5/8					446:453	CGB3/5/8	446:453	CGB3/5/8	446:453	CGB3, 5 and 8 code for an identical protein, CGB3/5/8, whereas CGB7 differs in three amino acids from CGB3/5/8.					
32767150	1	29	theme	chorionic	176:184	arg1	gonadotropin					186:197	human chorionic gonadotropin	170:197	human chorionic gonadotropin (hCG)	170:203	The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy.					
32767150	1	29	theme	chorionic	176:184	arg1	hCG					200:202	hCG	200:202	hCG	200:202	The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy.					
32767150	0	30	theme	O-glycosylation	7:21	arg1	patterns					23:30	N- and O-glycosylation patterns	0:30	N- and O-glycosylation patterns	0:30	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	0	31	theme	gonadotropin	111:122	arg1	subunit					135:141	the human chorionic gonadotropin (hCG) beta subunit	91:141	the human chorionic gonadotropin (hCG) beta subunit	91:141	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	1	32	theme	gonadotropin	186:197	arg1	role					212:215	its role	208:215	its role in supporting pregnancy	208:239	The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy.					
32767150	1	32	theme	gonadotropin	186:197	arg1	function					158:165	The classical function	144:165	The classical function of human chorionic gonadotropin (hCG)	144:203	The classical function of human chorionic gonadotropin (hCG) is its role in supporting pregnancy.					
32767150	0	33	theme	functional	36:45	arg1	testing					47:53	functional testing	36:53	functional testing	36:53	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	6	34	theme	variants	821:828	arg1	structures					759:768	the glycan structures	748:768	the glycan structures	748:768	Here, we investigate the glycan structures and possible functional differences of the two CGB variants.					
32767150	6	34	theme	variants	821:828	arg1	differences					794:804	possible functional differences	774:804	possible functional differences	774:804	Here, we investigate the glycan structures and possible functional differences of the two CGB variants.					
32767150	5	35	theme	tissue	582:587	arg1	patterns					600:607	very distinct tissue expression patterns	568:607	very distinct tissue expression patterns	568:607	We had observed earlier that CGB7 and CGB3/5/8 display very distinct tissue expression patterns and that the tumor suppressor and transcription factor p53 can activate expression of CGB7 but not of CGB3/5/8 genes.					
32767150	8	36	gly	O-glycosylation	957:971	arg1	CGB7					985:988	CGB7	985:988	CGB7	985:988	We found that N- and O-glycosylation patterns of CGB7 and CGB3/5/8 are quite similar.					
32767150	8	36	gly	O-glycosylation	957:971	arg1	CGB3/5/8					994:1001	CGB3/5/8	994:1001	CGB3/5/8	994:1001	We found that N- and O-glycosylation patterns of CGB7 and CGB3/5/8 are quite similar.					
32767150	5	37	theme	CGB3/5/8	711:718	arg1	genes					720:724	CGB3/5/8 genes	711:724	CGB3/5/8 genes	711:724	We had observed earlier that CGB7 and CGB3/5/8 display very distinct tissue expression patterns and that the tumor suppressor and transcription factor p53 can activate expression of CGB7 but not of CGB3/5/8 genes.					
32767150	6	38	theme	CGB	817:819	arg1	variants					821:828	the two CGB variants	809:828	the two CGB variants	809:828	Here, we investigate the glycan structures and possible functional differences of the two CGB variants.					
32767150	5	39	theme	expression	589:598	arg1	patterns					600:607	very distinct tissue expression patterns	568:607	very distinct tissue expression patterns	568:607	We had observed earlier that CGB7 and CGB3/5/8 display very distinct tissue expression patterns and that the tumor suppressor and transcription factor p53 can activate expression of CGB7 but not of CGB3/5/8 genes.					
32767150	8	40	theme	CGB7	985:988	arg1	patterns					973:980	N- and O-glycosylation patterns	950:980	N- and O-glycosylation patterns of CGB7 and CGB3/5/8	950:1001	We found that N- and O-glycosylation patterns of CGB7 and CGB3/5/8 are quite similar.					
32767150	10	41	theme	CGB7	1324:1327	arg1	activity					1312:1319	biological activity	1301:1319	biological activity of CGB7	1301:1327	This is the first time that biological activity of CGB7 is shown.					
32767150	5	42	theme	genes	720:724	arg1	expression					681:690	expression	681:690	expression of CGB7 but not of CGB3/5/8 genes	681:724	We had observed earlier that CGB7 and CGB3/5/8 display very distinct tissue expression patterns and that the tumor suppressor and transcription factor p53 can activate expression of CGB7 but not of CGB3/5/8 genes.					
32767150	0	43	theme	subunit	135:141	arg1	variants					79:86	CGB7 versus CGB3/5/8 variants	58:86	CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit	58:141	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	9	44	theme	pathway	1091:1097	arg1	activation					1066:1075	activation	1066:1075	activation of the ERK1/2 pathway	1066:1097	Functional assays were performed by testing activation of the ERK1/2 pathway and demonstrated that CGB7 and CGB5/5/8 appear to be functionally redundant isoforms, although a slight difference in the kinetics of ERK1/2 pathway activation was observed.					
32767150	0	45	gly	O-glycosylation	7:21	arg1	variants					79:86	CGB7 versus CGB3/5/8 variants	58:86	CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit	58:141	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	8	46	theme	O-glycosylation	957:971	arg1	patterns					973:980	N- and O-glycosylation patterns	950:980	N- and O-glycosylation patterns of CGB7 and CGB3/5/8	950:1001	We found that N- and O-glycosylation patterns of CGB7 and CGB3/5/8 are quite similar.					
32767150	7	47	theme	CGB7	908:911	arg1	proteins					926:933	recombinant CGA, CGB7 and CGB3/5/8 proteins	891:933	recombinant CGA, CGB7 and CGB3/5/8 proteins	891:933	To this end, we established a system to produce and isolate recombinant CGA, CGB7 and CGB3/5/8 proteins.					
32767150	0	48	theme	beta	130:133	arg1	subunit					135:141	the human chorionic gonadotropin (hCG) beta subunit	91:141	the human chorionic gonadotropin (hCG) beta subunit	91:141	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	6	49	theme	possible	774:781	arg1	differences					794:804	possible functional differences	774:804	possible functional differences	774:804	Here, we investigate the glycan structures and possible functional differences of the two CGB variants.					
32767150	9	50	theme	ERK1/2	1084:1089	arg1	pathway					1091:1097	the ERK1/2 pathway	1080:1097	the ERK1/2 pathway	1080:1097	Functional assays were performed by testing activation of the ERK1/2 pathway and demonstrated that CGB7 and CGB5/5/8 appear to be functionally redundant isoforms, although a slight difference in the kinetics of ERK1/2 pathway activation was observed.					
32767150	2	51	gly	glycosylated	282:293	arg1	beta					321:324	beta	321:324	beta (CGB)	321:330	hCG is a dimer consisting of two highly glycosylated subunits, alpha (CGA) and beta (CGB).					
32767150	2	51	gly	glycosylated	282:293	arg1	alpha					305:309	alpha	305:309	alpha (CGA)	305:315	hCG is a dimer consisting of two highly glycosylated subunits, alpha (CGA) and beta (CGB).					
32767150	2	51	gly	glycosylated	282:293	arg1	subunits					295:302	two highly glycosylated subunits	271:302	two highly glycosylated subunits	271:302	hCG is a dimer consisting of two highly glycosylated subunits, alpha (CGA) and beta (CGB).					
32767150	9	52	theme	slight	1196:1201	arg1	difference					1203:1212	a slight difference	1194:1212	a slight difference in the kinetics of ERK1/2 pathway activation	1194:1257	Functional assays were performed by testing activation of the ERK1/2 pathway and demonstrated that CGB7 and CGB5/5/8 appear to be functionally redundant isoforms, although a slight difference in the kinetics of ERK1/2 pathway activation was observed.					
32767150	5	53	theme	factor	657:662	arg1	p53					664:666	the tumor suppressor and transcription factor p53	618:666	p53	664:666	We had observed earlier that CGB7 and CGB3/5/8 display very distinct tissue expression patterns and that the tumor suppressor and transcription factor p53 can activate expression of CGB7 but not of CGB3/5/8 genes.					
32767150	2	54	theme	glycosylated	282:293	arg1	beta					321:324	beta	321:324	beta (CGB)	321:330	hCG is a dimer consisting of two highly glycosylated subunits, alpha (CGA) and beta (CGB).					
32767150	2	54	theme	glycosylated	282:293	arg1	alpha					305:309	alpha	305:309	alpha (CGA)	305:315	hCG is a dimer consisting of two highly glycosylated subunits, alpha (CGA) and beta (CGB).					
32767150	2	54	theme	glycosylated	282:293	arg1	subunits					295:302	two highly glycosylated subunits	271:302	two highly glycosylated subunits	271:302	hCG is a dimer consisting of two highly glycosylated subunits, alpha (CGA) and beta (CGB).					
32767150	11	55	theme	cell	1476:1479	arg1	key					1513:1515	key	1513:1515	key	1513:1515	In summary, the results lead to the hypothesis that CGB7 and CGB3/5/8 do not hold significant functional differences but that timing and cell type of their expression is the key for understanding their divergent evolution.					
32767150	11	55	theme	cell	1476:1479	arg1	timing					1465:1470	timing	1465:1470	timing	1465:1470	In summary, the results lead to the hypothesis that CGB7 and CGB3/5/8 do not hold significant functional differences but that timing and cell type of their expression is the key for understanding their divergent evolution.					
32767150	11	55	theme	cell	1476:1479	arg1	type					1481:1484	cell type	1476:1484	cell type	1476:1484	In summary, the results lead to the hypothesis that CGB7 and CGB3/5/8 do not hold significant functional differences but that timing and cell type of their expression is the key for understanding their divergent evolution.					
32767150	9	56	theme	ERK1/2	1233:1238	arg1	activation					1248:1257	ERK1/2 pathway activation	1233:1257	ERK1/2 pathway activation	1233:1257	Functional assays were performed by testing activation of the ERK1/2 pathway and demonstrated that CGB7 and CGB5/5/8 appear to be functionally redundant isoforms, although a slight difference in the kinetics of ERK1/2 pathway activation was observed.					
32767150	5	57	theme	tumor	622:626	arg1	suppressor					628:637	the tumor suppressor and transcription factor p53	618:666	suppressor	628:637	We had observed earlier that CGB7 and CGB3/5/8 display very distinct tissue expression patterns and that the tumor suppressor and transcription factor p53 can activate expression of CGB7 but not of CGB3/5/8 genes.					
32767150	9	58	theme	pathway	1240:1246	arg1	activation					1248:1257	ERK1/2 pathway activation	1233:1257	ERK1/2 pathway activation	1233:1257	Functional assays were performed by testing activation of the ERK1/2 pathway and demonstrated that CGB7 and CGB5/5/8 appear to be functionally redundant isoforms, although a slight difference in the kinetics of ERK1/2 pathway activation was observed.					
32767150	0	59	theme	CGB7	58:61	arg1	variants					79:86	CGB7 versus CGB3/5/8 variants	58:86	CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit	58:141	N- and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit.					
32767150	9	60	theme	activation	1248:1257	arg1	kinetics					1221:1228	the kinetics	1217:1228	the kinetics of ERK1/2 pathway activation	1217:1257	Functional assays were performed by testing activation of the ERK1/2 pathway and demonstrated that CGB7 and CGB5/5/8 appear to be functionally redundant isoforms, although a slight difference in the kinetics of ERK1/2 pathway activation was observed.					
32767150	11	61	theme	significant	1421:1431	arg1	differences					1444:1454	significant functional differences	1421:1454	significant functional differences	1421:1454	In summary, the results lead to the hypothesis that CGB7 and CGB3/5/8 do not hold significant functional differences but that timing and cell type of their expression is the key for understanding their divergent evolution.					
32767150	4	62	theme	amino	486:490	arg1	acids					492:496	three amino acids	480:496	three amino acids	480:496	CGB3, 5 and 8 code for an identical protein, CGB3/5/8, whereas CGB7 differs in three amino acids from CGB3/5/8.					
32815134	2	0	theme	gel	379:381	arg1	chromatography					394:407	gel permeation chromatography	379:407	gel permeation chromatography	379:407	The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography.					
32815134	3	1	from	basis	579:583	arg1	pathogenesis					638:649	bacterial pathogenesis	628:649	bacterial pathogenesis	628:649	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	3	2	from	isolation	443:451	arg1	strains					496:502	T. forsythia strains	483:502	T. forsythia strains with diverse O-glycan structures	483:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	1	3	theme	detailed	117:124	arg1	protocol					139:146	a detailed purification protocol	115:146	a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia	115:241	The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia.					
32815134	1	4	theme	surface-layer	156:168	arg1	glycoproteins					180:192	the surface-layer (S-layer) glycoproteins	152:192	the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia	152:241	The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia.					
32815134	1	5	gly	glycoproteins	180:192	arg1	glycoproteins					180:192	the surface-layer (S-layer) glycoproteins	152:192	the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia	152:241	The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia.					
32815134	2	6	theme	based	277:281	arg1	solubilization					283:296	detergent based solubilization	267:296	detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography	267:407	The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography.					
32815134	1	7	theme	S-layer	171:177	arg1	glycoproteins					180:192	the surface-layer (S-layer) glycoproteins	152:192	the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia	152:241	The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia.					
32815134	3	8	theme	protein	601:607	arg1	O-glycosylation					609:623	protein O-glycosylation	601:623	protein O-glycosylation	601:623	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	3	9	theme	S-layer	456:462	arg1	glycoproteins					464:476	S-layer glycoproteins	456:476	S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures	456:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	0	10	theme	forsythia	27:35	arg1	Proteins					61:68	Tannerella forsythia Surface-Layer (S-Layer) Proteins	16:68	Tannerella forsythia Surface-Layer (S-Layer) Proteins	16:68	Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins.					
32815134	1	11	theme	chapter	93:99	arg1	objective					75:83	The objective	71:83	The objective of this chapter	71:99	The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia.					
32815134	0	12	theme	Tannerella	16:25	arg1	Proteins					61:68	Tannerella forsythia Surface-Layer (S-Layer) Proteins	16:68	Tannerella forsythia Surface-Layer (S-Layer) Proteins	16:68	Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins.					
32815134	2	13	theme	bacterial	305:313	arg1	S-layer					315:321	the bacterial S-layer	301:321	the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography	301:407	The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography.					
32815134	3	14	with	strains	496:502	arg1	structures					526:535	diverse O-glycan structures	509:535	diverse O-glycan structures	509:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	3	15	theme	bacterial	628:636	arg1	pathogenesis					638:649	bacterial pathogenesis	628:649	bacterial pathogenesis	628:649	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	3	16	theme	T.	483:484	arg1	strains					496:502	T. forsythia strains	483:502	T. forsythia strains with diverse O-glycan structures	483:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	0	17	theme	Surface-Layer	37:49	arg1	Proteins					61:68	Tannerella forsythia Surface-Layer (S-Layer) Proteins	16:68	Tannerella forsythia Surface-Layer (S-Layer) Proteins	16:68	Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins.					
32815134	1	18	theme	periodontal	201:211	arg1	forsythia					233:241	the periodontal pathogen Tannerella forsythia	197:241	the periodontal pathogen Tannerella forsythia	197:241	The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia.					
32815134	3	19	theme	glycoproteins	464:476	arg1	isolation					443:451	the isolation	439:451	the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures	439:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	3	20	theme	O-glycosylation	609:623	arg1	basis					579:583	the biochemical basis	563:583	the biochemical basis	563:583	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	3	20	theme	O-glycosylation	609:623	arg1	role					593:596	the role	589:596	the role of protein O-glycosylation in bacterial pathogenesis	589:649	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	0	21	theme	S-Layer	52:58	arg1	Proteins					61:68	Tannerella forsythia Surface-Layer (S-Layer) Proteins	16:68	Tannerella forsythia Surface-Layer (S-Layer) Proteins	16:68	Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins.					
32815134	3	22	theme	diverse	509:515	arg1	structures					526:535	diverse O-glycan structures	509:535	diverse O-glycan structures	509:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	3	23	from	strains	496:502	arg1	glycoproteins					464:476	S-layer glycoproteins	456:476	S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures	456:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	3	23	from	strains	496:502	arg1	isolation					443:451	the isolation	439:451	the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures	439:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	2	24	theme	gradient	351:358	arg1	centrifugation					360:373	cesium chloride gradient centrifugation	335:373	cesium chloride gradient centrifugation	335:373	The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography.					
32815134	3	25	from	role	593:596	arg1	pathogenesis					638:649	bacterial pathogenesis	628:649	bacterial pathogenesis	628:649	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	2	26	theme	detergent	267:275	arg1	solubilization					283:296	detergent based solubilization	267:296	detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography	267:407	The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography.					
32815134	2	27	theme	chloride	342:349	arg1	centrifugation					360:373	cesium chloride gradient centrifugation	335:373	cesium chloride gradient centrifugation	335:373	The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography.					
32815134	2	28	theme	S-layer	315:321	arg1	solubilization					283:296	detergent based solubilization	267:296	detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography	267:407	The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography.					
32815134	1	29	theme	pathogen	213:220	arg1	forsythia					233:241	the periodontal pathogen Tannerella forsythia	197:241	the periodontal pathogen Tannerella forsythia	197:241	The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia.					
32815134	3	30	theme	biochemical	567:577	arg1	basis					579:583	the biochemical basis	563:583	the biochemical basis	563:583	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	3	31	gly	glycoproteins	464:476	arg1	glycoproteins					464:476	S-layer glycoproteins	456:476	S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures	456:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	2	32	theme	cesium	335:340	arg1	centrifugation					360:373	cesium chloride gradient centrifugation	335:373	cesium chloride gradient centrifugation	335:373	The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography.					
32815134	1	33	theme	Tannerella	222:231	arg1	forsythia					233:241	the periodontal pathogen Tannerella forsythia	197:241	the periodontal pathogen Tannerella forsythia	197:241	The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia.					
32815134	3	34	theme	forsythia	486:494	arg1	strains					496:502	T. forsythia strains	483:502	T. forsythia strains with diverse O-glycan structures	483:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	1	35	theme	purification	126:137	arg1	protocol					139:146	a detailed purification protocol	115:146	a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia	115:241	The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia.					
32815134	3	36	theme	O-glycan	517:524	arg1	structures					526:535	diverse O-glycan structures	509:535	diverse O-glycan structures	509:535	The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.					
32815134	1	37	theme	forsythia	233:241	arg1	glycoproteins					180:192	the surface-layer (S-layer) glycoproteins	152:192	the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia	152:241	The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia.					
32815134	0	38	theme	Proteins	61:68	arg1	Purification					0:11	Purification	0:11	Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins	0:68	Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins.					
32815134	2	39	theme	permeation	383:392	arg1	chromatography					394:407	gel permeation chromatography	379:407	gel permeation chromatography	379:407	The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography.					
34232070	0	0	theme	PGT121	34:39	arg1	Ability					9:15	Enhanced Ability	0:15	Enhanced Ability of Plant-Derived PGT121	0:39	Enhanced Ability of Plant-Derived PGT121 Glycovariants To Eliminate HIV-1-Infected Cells.					
34232070	7	1	theme	HIV-1-infected	1324:1337	arg1	cells					1339:1343	primary HIV-1-infected cells	1316:1343	primary HIV-1-infected cells	1316:1343	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	7	2	theme	afucosylated	1185:1196	arg1	PGT121					1198:1203	afucosylated PGT121	1185:1203	afucosylated PGT121	1185:1203	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	10	3	theme	clinical	1796:1803	arg1	trials					1805:1810	clinical trials	1796:1810	clinical trials	1796:1810	IMPORTANCE PGT121 is a highly potent bNAb and its antiviral activities for HIV-1 prevention and therapy are currently being evaluated in clinical trials.					
34232070	1	4	theme	antibodies	127:136	arg1	activity					94:101	The activity	90:101	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1	90:160	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	12	5	theme	disease	2097:2103	arg1	progression					2105:2115	infected cell clearance and slow disease progression	2064:2115	progression	2105:2115	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	7	6	gly	afucosylated	1185:1196	arg1	PGT121					1198:1203	afucosylated PGT121	1185:1203	afucosylated PGT121	1185:1203	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	2	7	theme	passive	316:322	arg1	administration					324:337	passive administration	316:337	passive administration of bNAbs	316:346	Several in vivo studies have suggested that passive administration of bNAbs represents a valuable strategy for the prevention or treatment of HIV-1.					
34232070	4	8	theme	valuable	675:682	arg1	therapeutics					684:695	valuable therapeutics	675:695	valuable therapeutics	675:695	Production of antibodies in plants permits low-cost and large-scale production of valuable therapeutics; furthermore, pertinent to this work, it also includes an advanced glycoengineering platform.					
34232070	7	9	theme	FcγRIIIa	1235:1242	arg1	interaction					1244:1254	significantly enhanced FcγRIIIa interaction	1212:1254	significantly enhanced FcγRIIIa interaction	1212:1254	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	5	10	theme	potent	879:884	arg1	PGT121					892:897	PGT121	892:897	PGT121	892:897	In this study, we used Nicotiana benthamiana to produce different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles and evaluated their antiviral activities.					
34232070	5	10	theme	potent	879:884	arg1	bNAb					886:889	a potent bNAb	877:889	a potent bNAb	877:889	In this study, we used Nicotiana benthamiana to produce different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles and evaluated their antiviral activities.					
34232070	12	11	theme	cell	2073:2076	arg1	clearance					2078:2086	infected cell clearance and slow disease progression	2064:2115	clearance	2078:2086	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	11	12	from	importance	1817:1826	arg1	cells					1884:1888	clearing HIV-1-infected cells	1860:1888	clearing HIV-1-infected cells	1860:1888	The importance of its Fc-effector functions in clearing HIV-1-infected cells is also under investigation.					
34232070	10	13	theme	potent	1689:1694	arg1	bNAb					1696:1699	a highly potent bNAb	1680:1699	a highly potent bNAb	1680:1699	IMPORTANCE PGT121 is a highly potent bNAb and its antiviral activities for HIV-1 prevention and therapy are currently being evaluated in clinical trials.					
34232070	10	13	theme	potent	1689:1694	arg1	IMPORTANCE					1659:1668	IMPORTANCE PGT121	1659:1675	IMPORTANCE PGT121	1659:1675	IMPORTANCE PGT121 is a highly potent bNAb and its antiviral activities for HIV-1 prevention and therapy are currently being evaluated in clinical trials.					
34232070	9	14	theme	glycan	1564:1569	arg1	fucosylation					1571:1582	the Fc N-linked glycan fucosylation	1548:1582	the Fc N-linked glycan fucosylation	1548:1582	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	2	15	theme	in	280:281	arg1	studies					288:294	Several in vivo studies	272:294	Several in vivo studies	272:294	Several in vivo studies have suggested that passive administration of bNAbs represents a valuable strategy for the prevention or treatment of HIV-1.					
34232070	5	16	theme	antiviral	951:959	arg1	activities					961:970	their antiviral activities	945:970	their antiviral activities	945:970	In this study, we used Nicotiana benthamiana to produce different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles and evaluated their antiviral activities.					
34232070	8	17	theme	overall	1386:1392	arg1	profiles					1410:1417	the overall galactosylation profiles	1382:1417	the overall galactosylation profiles of plant PGT121	1382:1433	However, the overall galactosylation profiles of plant PGT121 did not affect ADCC activities against infected primary CD4+ T cells.					
34232070	7	18	theme	primary	1316:1322	arg1	cells					1339:1343	primary HIV-1-infected cells	1316:1343	primary HIV-1-infected cells	1316:1343	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	5	19	theme	near-homogeneous	905:920	arg1	profiles					922:929	near-homogeneous profiles	905:929	near-homogeneous profiles	905:929	In this study, we used Nicotiana benthamiana to produce different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles and evaluated their antiviral activities.					
34232070	11	20	theme	clearing	1860:1867	arg1	cells					1884:1888	clearing HIV-1-infected cells	1860:1888	clearing HIV-1-infected cells	1860:1888	The importance of its Fc-effector functions in clearing HIV-1-infected cells is also under investigation.					
34232070	13	21	theme	HIV-1	2212:2216	arg1	replication					2218:2228	HIV-1 replication	2212:2228	HIV-1 replication	2212:2228	Future studies to evaluate the potential of plant-produced afucosylated PGT121 in controlling HIV-1 replication in vivo are warranted.					
34232070	9	22	theme	worthwhile	1599:1608	arg1	strategy					1610:1617	a worthwhile strategy	1597:1617	a worthwhile strategy to boost its Fc-effector functionality	1597:1656	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	9	22	theme	worthwhile	1599:1608	arg1	abrogation					1534:1543	the abrogation	1530:1543	the abrogation of the Fc N-linked glycan fucosylation of PGT121	1530:1592	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	8	23	theme	plant	1422:1426	arg1	PGT121					1428:1433	plant PGT121	1422:1433	plant PGT121	1422:1433	However, the overall galactosylation profiles of plant PGT121 did not affect ADCC activities against infected primary CD4+ T cells.					
34232070	4	24	theme	glycoengineering	764:779	arg1	platform					781:788	an advanced glycoengineering platform	752:788	an advanced glycoengineering platform	752:788	Production of antibodies in plants permits low-cost and large-scale production of valuable therapeutics; furthermore, pertinent to this work, it also includes an advanced glycoengineering platform.					
34232070	11	25	theme	functions	1847:1855	arg1	importance					1817:1826	The importance	1813:1826	The importance of its Fc-effector functions in clearing HIV-1-infected cells	1813:1888	The importance of its Fc-effector functions in clearing HIV-1-infected cells is also under investigation.					
34232070	12	26	theme	therapeutic	2030:2040	arg1	context					2042:2048	a therapeutic context	2028:2048	a therapeutic context	2028:2048	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	6	27	theme	overall	1026:1032	arg1	structure					1034:1042	overall structure	1026:1042	overall structure	1026:1042	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	12	28	gly	afucosylated	1976:1987	arg1	MAbs					1996:1999	afucosylated PGT121 MAbs	1976:1999	afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression	1976:2115	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	10	29	theme	HIV-1	1734:1738	arg1	prevention					1740:1749	HIV-1 prevention	1734:1749	HIV-1 prevention	1734:1749	IMPORTANCE PGT121 is a highly potent bNAb and its antiviral activities for HIV-1 prevention and therapy are currently being evaluated in clinical trials.					
34232070	4	30	from	Production	593:602	arg1	plants					621:626	plants	621:626	plants	621:626	Production of antibodies in plants permits low-cost and large-scale production of valuable therapeutics; furthermore, pertinent to this work, it also includes an advanced glycoengineering platform.					
34232070	13	31	theme	afucosylated	2177:2188	arg1	PGT121					2190:2195	plant-produced afucosylated PGT121	2162:2195	plant-produced afucosylated PGT121	2162:2195	Future studies to evaluate the potential of plant-produced afucosylated PGT121 in controlling HIV-1 replication in vivo are warranted.					
34232070	6	32	theme	Fc	1074:1075	arg1	regions					1077:1083	Fc regions	1074:1083	Fc regions for these plant-derived Abs and mammalian cell-derived Abs	1074:1142	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	6	33	theme	cell-derived	1127:1138	arg1	Abs					1140:1142	mammalian cell-derived Abs	1117:1142	mammalian cell-derived Abs	1117:1142	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	4	34	theme	advanced	755:762	arg1	platform					781:788	an advanced glycoengineering platform	752:788	an advanced glycoengineering platform	752:788	Production of antibodies in plants permits low-cost and large-scale production of valuable therapeutics; furthermore, pertinent to this work, it also includes an advanced glycoengineering platform.					
34232070	0	35	theme	Enhanced	0:7	arg1	Ability					9:15	Enhanced Ability	0:15	Enhanced Ability of Plant-Derived PGT121	0:39	Enhanced Ability of Plant-Derived PGT121 Glycovariants To Eliminate HIV-1-Infected Cells.					
34232070	12	36	theme	PGT121	1989:1994	arg1	MAbs					1996:1999	afucosylated PGT121 MAbs	1976:1999	afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression	1976:2115	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	5	37	theme	different	847:855	arg1	Fc-glycovariants					857:872	different Fc-glycovariants	847:872	different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles	847:929	In this study, we used Nicotiana benthamiana to produce different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles and evaluated their antiviral activities.					
34232070	5	38	used	used	809:812	arg2	we					806:807	we	806:807	we	806:807	In this study, we used Nicotiana benthamiana to produce different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles and evaluated their antiviral activities.					
34232070	1	39	theme	neutralizing	114:125	arg1	bNAbs					139:143	bNAbs	139:143	bNAbs	139:143	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	1	39	theme	neutralizing	114:125	arg1	antibodies					127:136	broadly neutralizing antibodies	106:136	broadly neutralizing antibodies (bNAbs) targeting HIV-1	106:160	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	6	40	theme	plant-derived	1095:1107	arg1	Abs					1109:1111	these plant-derived Abs	1089:1111	these plant-derived Abs	1089:1111	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	8	41	theme	infected	1474:1481	arg1	cells					1498:1502	infected primary CD4+ T cells	1474:1502	infected primary CD4+ T cells	1474:1502	However, the overall galactosylation profiles of plant PGT121 did not affect ADCC activities against infected primary CD4+ T cells.					
34232070	5	42	theme	bNAb	886:889	arg1	Fc-glycovariants					857:872	different Fc-glycovariants	847:872	different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles	847:929	In this study, we used Nicotiana benthamiana to produce different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles and evaluated their antiviral activities.					
34232070	7	43	theme	enhanced	1226:1233	arg1	interaction					1244:1254	significantly enhanced FcγRIIIa interaction	1212:1254	significantly enhanced FcγRIIIa interaction	1212:1254	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	0	44	theme	HIV-1-Infected	68:81	arg1	Cells					83:87	HIV-1-Infected Cells	68:87	HIV-1-Infected Cells	68:87	Enhanced Ability of Plant-Derived PGT121 Glycovariants To Eliminate HIV-1-Infected Cells.					
34232070	8	45	theme	CD4+	1491:1494	arg1	cells					1498:1502	infected primary CD4+ T cells	1474:1502	infected primary CD4+ T cells	1474:1502	However, the overall galactosylation profiles of plant PGT121 did not affect ADCC activities against infected primary CD4+ T cells.					
34232070	6	46	gly	glycosylation	1048:1060	arg1	regions					1077:1083	Fc regions	1074:1083	Fc regions for these plant-derived Abs and mammalian cell-derived Abs	1074:1142	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	5	47	theme	Nicotiana	814:822	arg1	benthamiana					824:834	Nicotiana benthamiana	814:834	Nicotiana benthamiana	814:834	In this study, we used Nicotiana benthamiana to produce different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles and evaluated their antiviral activities.					
34232070	5	48	with	bNAb	886:889	arg1	profiles					922:929	near-homogeneous profiles	905:929	near-homogeneous profiles	905:929	In this study, we used Nicotiana benthamiana to produce different Fc-glycovariants of a potent bNAb, PGT121, with near-homogeneous profiles and evaluated their antiviral activities.					
34232070	12	49	theme	Fc-effector	1950:1960	arg1	activities					1962:1971	enhanced Fc-effector activities	1941:1971	enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression	1941:2115	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	1	50	theme	HIV-1-infected	250:263	arg1	cells					265:269	HIV-1-infected cells	250:269	HIV-1-infected cells	250:269	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	4	51	theme	low-cost	636:643	arg1	production					661:670	low-cost and large-scale production	636:670	low-cost and large-scale production of valuable therapeutics	636:695	Production of antibodies in plants permits low-cost and large-scale production of valuable therapeutics; furthermore, pertinent to this work, it also includes an advanced glycoengineering platform.					
34232070	6	52	link	cell-derived	1127:1138	arg1	Abs					1140:1142	mammalian cell-derived Abs	1117:1142	mammalian cell-derived Abs	1117:1142	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	7	53	theme	Fc-effector	1161:1171	arg1	activities					1173:1182	Fc-effector activities	1161:1182	Fc-effector activities	1161:1182	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	3	54	theme	antibodies	552:561	arg1	quantities					538:547	the large quantities	528:547	the large quantities of antibodies required for clinical trials	528:590	In addition, different strategies are currently being tested to scale up the production of bNAbs to obtain the large quantities of antibodies required for clinical trials.					
34232070	4	55	theme	antibodies	607:616	arg1	Production					593:602	Production	593:602	Production of antibodies in plants	593:626	Production of antibodies in plants permits low-cost and large-scale production of valuable therapeutics; furthermore, pertinent to this work, it also includes an advanced glycoengineering platform.					
34232070	1	56	theme	pleiotropic	173:183	arg1	neutralization					212:225	viral neutralization	206:225	viral neutralization	206:225	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	1	56	theme	pleiotropic	173:183	arg1	elimination					235:245	the elimination	231:245	the elimination of HIV-1-infected cells	231:269	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	1	56	theme	pleiotropic	173:183	arg1	functions					185:193	pleiotropic functions	173:193	pleiotropic functions	173:193	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	12	57	from	important	2015:2023	arg1	context					2042:2048	a therapeutic context	2028:2048	a therapeutic context	2028:2048	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	4	58	theme	therapeutics	684:695	arg1	production					661:670	low-cost and large-scale production	636:670	low-cost and large-scale production of valuable therapeutics	636:695	Production of antibodies in plants permits low-cost and large-scale production of valuable therapeutics; furthermore, pertinent to this work, it also includes an advanced glycoengineering platform.					
34232070	6	59	link	plant-derived	1095:1107	arg1	Abs					1109:1111	these plant-derived Abs	1089:1111	these plant-derived Abs	1089:1111	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	2	60	theme	bNAbs	342:346	arg1	administration					324:337	passive administration	316:337	passive administration of bNAbs	316:346	Several in vivo studies have suggested that passive administration of bNAbs represents a valuable strategy for the prevention or treatment of HIV-1.					
34232070	2	61	dep	in	280:281	arg1	vivo					283:286	vivo	283:286	vivo	283:286	Several in vivo studies have suggested that passive administration of bNAbs represents a valuable strategy for the prevention or treatment of HIV-1.					
34232070	1	62	theme	viral	206:210	arg1	neutralization					212:225	viral neutralization	206:225	viral neutralization	206:225	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	12	63	theme	slow	2092:2095	arg1	progression					2105:2115	infected cell clearance and slow disease progression	2064:2115	progression	2105:2115	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	6	64	theme	close	1006:1010	arg1	similarity					1012:1021	a close similarity	1004:1021	a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs	1004:1142	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	13	65	gly	afucosylated	2177:2188	arg1	PGT121					2190:2195	plant-produced afucosylated PGT121	2162:2195	plant-produced afucosylated PGT121	2162:2195	Future studies to evaluate the potential of plant-produced afucosylated PGT121 in controlling HIV-1 replication in vivo are warranted.					
34232070	4	66	theme	large-scale	649:659	arg1	production					661:670	low-cost and large-scale production	636:670	low-cost and large-scale production of valuable therapeutics	636:695	Production of antibodies in plants permits low-cost and large-scale production of valuable therapeutics; furthermore, pertinent to this work, it also includes an advanced glycoengineering platform.					
34232070	3	67	theme	bNAbs	512:516	arg1	production					498:507	the production	494:507	the production of bNAbs to obtain the large quantities of antibodies required for clinical trials	494:590	In addition, different strategies are currently being tested to scale up the production of bNAbs to obtain the large quantities of antibodies required for clinical trials.					
34232070	9	68	theme	N-linked	1555:1562	arg1	fucosylation					1571:1582	the Fc N-linked glycan fucosylation	1548:1582	the Fc N-linked glycan fucosylation	1548:1582	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	12	69	theme	infected	2064:2071	arg1	clearance					2078:2086	infected cell clearance and slow disease progression	2064:2115	clearance	2078:2086	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	6	70	theme	Structural	973:982	arg1	analyses					984:991	Structural analyses	973:991	Structural analyses	973:991	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	9	71	theme	fucosylation	1571:1582	arg1	strategy					1610:1617	a worthwhile strategy	1597:1617	a worthwhile strategy to boost its Fc-effector functionality	1597:1656	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	9	71	theme	fucosylation	1571:1582	arg1	abrogation					1534:1543	the abrogation	1530:1543	the abrogation of the Fc N-linked glycan fucosylation of PGT121	1530:1592	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	6	72	theme	regions	1077:1083	arg1	structure					1034:1042	overall structure	1026:1042	overall structure	1026:1042	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	6	72	theme	regions	1077:1083	arg1	patterns					1062:1069	glycosylation patterns	1048:1069	glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs	1048:1142	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	11	73	theme	Fc-effector	1835:1845	arg1	functions					1847:1855	its Fc-effector functions	1831:1855	its Fc-effector functions	1831:1855	The importance of its Fc-effector functions in clearing HIV-1-infected cells is also under investigation.					
34232070	2	74	theme	Several	272:278	arg1	studies					288:294	Several in vivo studies	272:294	Several in vivo studies	272:294	Several in vivo studies have suggested that passive administration of bNAbs represents a valuable strategy for the prevention or treatment of HIV-1.					
34232070	9	75	theme	PGT121	1587:1592	arg1	strategy					1610:1617	a worthwhile strategy	1597:1617	a worthwhile strategy to boost its Fc-effector functionality	1597:1656	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	9	75	theme	PGT121	1587:1592	arg1	abrogation					1534:1543	the abrogation	1530:1543	the abrogation of the Fc N-linked glycan fucosylation of PGT121	1530:1592	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	12	76	from	context	2042:2048	arg1	important					2015:2023	important	2015:2023	important	2015:2023	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	6	77	theme	glycosylation	1048:1060	arg1	patterns					1062:1069	glycosylation patterns	1048:1069	glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs	1048:1142	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	8	78	theme	galactosylation	1394:1408	arg1	profiles					1410:1417	the overall galactosylation profiles	1382:1417	the overall galactosylation profiles of plant PGT121	1382:1433	However, the overall galactosylation profiles of plant PGT121 did not affect ADCC activities against infected primary CD4+ T cells.					
34232070	11	79	theme	HIV-1-infected	1869:1882	arg1	cells					1884:1888	clearing HIV-1-infected cells	1860:1888	clearing HIV-1-infected cells	1860:1888	The importance of its Fc-effector functions in clearing HIV-1-infected cells is also under investigation.					
34232070	3	80	theme	clinical	576:583	arg1	trials					585:590	clinical trials	576:590	clinical trials	576:590	In addition, different strategies are currently being tested to scale up the production of bNAbs to obtain the large quantities of antibodies required for clinical trials.					
34232070	7	81	theme	dependent	1269:1277	arg1	cytotoxicity					1288:1299	antibody dependent cellular cytotoxicity	1260:1299	antibody dependent cellular cytotoxicity (ADCC)	1260:1306	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	7	81	theme	dependent	1269:1277	arg1	ADCC					1302:1305	ADCC	1302:1305	ADCC	1302:1305	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	13	82	theme	plant-produced	2162:2175	arg1	PGT121					2190:2195	plant-produced afucosylated PGT121	2162:2195	plant-produced afucosylated PGT121	2162:2195	Future studies to evaluate the potential of plant-produced afucosylated PGT121 in controlling HIV-1 replication in vivo are warranted.					
34232070	2	83	dep	prevention	387:396	arg1	the					383:385	the	383:385	the	383:385	Several in vivo studies have suggested that passive administration of bNAbs represents a valuable strategy for the prevention or treatment of HIV-1.					
34232070	2	84	theme	HIV-1	414:418	arg1	treatment					401:409	treatment	401:409	treatment	401:409	Several in vivo studies have suggested that passive administration of bNAbs represents a valuable strategy for the prevention or treatment of HIV-1.					
34232070	2	84	theme	HIV-1	414:418	arg1	prevention					387:396	prevention	387:396	prevention	387:396	Several in vivo studies have suggested that passive administration of bNAbs represents a valuable strategy for the prevention or treatment of HIV-1.					
34232070	13	85	theme	PGT121	2190:2195	arg1	potential					2149:2157	the potential	2145:2157	the potential of plant-produced afucosylated PGT121 in controlling HIV-1 replication in vivo	2145:2236	Future studies to evaluate the potential of plant-produced afucosylated PGT121 in controlling HIV-1 replication in vivo are warranted.					
34232070	9	86	theme	Fc-effector	1632:1642	arg1	functionality					1644:1656	its Fc-effector functionality	1628:1656	its Fc-effector functionality	1628:1656	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	6	87	theme	mammalian	1117:1125	arg1	Abs					1140:1142	mammalian cell-derived Abs	1117:1142	mammalian cell-derived Abs	1117:1142	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	10	88	theme	antiviral	1709:1717	arg1	activities					1719:1728	its antiviral activities	1705:1728	its antiviral activities for HIV-1 prevention and therapy	1705:1761	IMPORTANCE PGT121 is a highly potent bNAb and its antiviral activities for HIV-1 prevention and therapy are currently being evaluated in clinical trials.					
34232070	0	89	theme	Plant-Derived	20:32	arg1	PGT121					34:39	Plant-Derived PGT121	20:39	Plant-Derived PGT121	20:39	Enhanced Ability of Plant-Derived PGT121 Glycovariants To Eliminate HIV-1-Infected Cells.					
34232070	12	90	theme	afucosylated	1976:1987	arg1	MAbs					1996:1999	afucosylated PGT121 MAbs	1976:1999	afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression	1976:2115	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	1	91	theme	broadly	106:112	arg1	bNAbs					139:143	bNAbs	139:143	bNAbs	139:143	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	1	91	theme	broadly	106:112	arg1	antibodies					127:136	broadly neutralizing antibodies	106:136	broadly neutralizing antibodies (bNAbs) targeting HIV-1	106:160	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	13	92	dep	studies	2125:2131	arg1	evaluate					2136:2143	evaluate	2136:2143	to evaluate the potential of plant-produced afucosylated PGT121 in controlling HIV-1 replication in vivo	2133:2236	Future studies to evaluate the potential of plant-produced afucosylated PGT121 in controlling HIV-1 replication in vivo are warranted.					
34232070	8	93	theme	ADCC	1450:1453	arg1	activities					1455:1464	ADCC activities	1450:1464	ADCC activities against infected primary CD4+ T cells	1450:1502	However, the overall galactosylation profiles of plant PGT121 did not affect ADCC activities against infected primary CD4+ T cells.					
34232070	12	94	theme	MAbs	1996:1999	arg1	activities					1962:1971	enhanced Fc-effector activities	1941:1971	enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression	1941:2115	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	3	95	theme	different	434:442	arg1	strategies					444:453	different strategies	434:453	different strategies	434:453	In addition, different strategies are currently being tested to scale up the production of bNAbs to obtain the large quantities of antibodies required for clinical trials.					
34232070	6	96	from	similarity	1012:1021	arg1	structure					1034:1042	overall structure	1026:1042	overall structure	1026:1042	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	6	96	from	similarity	1012:1021	arg1	patterns					1062:1069	glycosylation patterns	1048:1069	glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs	1048:1142	Structural analyses identified a close similarity in overall structure and glycosylation patterns of Fc regions for these plant-derived Abs and mammalian cell-derived Abs.					
34232070	7	97	theme	cellular	1279:1286	arg1	cytotoxicity					1288:1299	antibody dependent cellular cytotoxicity	1260:1299	antibody dependent cellular cytotoxicity (ADCC)	1260:1306	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	7	97	theme	cellular	1279:1286	arg1	ADCC					1302:1305	ADCC	1302:1305	ADCC	1302:1305	When tested for Fc-effector activities, afucosylated PGT121 showed significantly enhanced FcγRIIIa interaction and antibody dependent cellular cytotoxicity (ADCC) against primary HIV-1-infected cells, both in vitro and ex vivo.					
34232070	12	98	theme	enhanced	1941:1948	arg1	activities					1962:1971	enhanced Fc-effector activities	1941:1971	enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression	1941:2115	Our results highlight enhanced Fc-effector activities of afucosylated PGT121 MAbs that could be important in a therapeutic context to accelerate infected cell clearance and slow disease progression.					
34232070	8	99	theme	primary	1483:1489	arg1	cells					1498:1502	infected primary CD4+ T cells	1474:1502	infected primary CD4+ T cells	1474:1502	However, the overall galactosylation profiles of plant PGT121 did not affect ADCC activities against infected primary CD4+ T cells.					
34232070	2	100	theme	valuable	361:368	arg1	strategy					370:377	a valuable strategy	359:377	a valuable strategy for the prevention or treatment of HIV-1	359:418	Several in vivo studies have suggested that passive administration of bNAbs represents a valuable strategy for the prevention or treatment of HIV-1.					
34232070	9	101	link	N-linked	1555:1562	arg1	fucosylation					1571:1582	the Fc N-linked glycan fucosylation	1548:1582	the Fc N-linked glycan fucosylation	1548:1582	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	8	102	theme	PGT121	1428:1433	arg1	profiles					1410:1417	the overall galactosylation profiles	1382:1417	the overall galactosylation profiles of plant PGT121	1382:1433	However, the overall galactosylation profiles of plant PGT121 did not affect ADCC activities against infected primary CD4+ T cells.					
34232070	8	103	theme	T	1496:1496	arg1	cells					1498:1502	infected primary CD4+ T cells	1474:1502	infected primary CD4+ T cells	1474:1502	However, the overall galactosylation profiles of plant PGT121 did not affect ADCC activities against infected primary CD4+ T cells.					
34232070	9	104	theme	Fc	1552:1553	arg1	fucosylation					1571:1582	the Fc N-linked glycan fucosylation	1548:1582	the Fc N-linked glycan fucosylation	1548:1582	Our results suggest that the abrogation of the Fc N-linked glycan fucosylation of PGT121 is a worthwhile strategy to boost its Fc-effector functionality.					
34232070	13	105	theme	Future	2118:2123	arg1	studies					2125:2131	Future studies	2118:2131	Future studies to evaluate the potential of plant-produced afucosylated PGT121 in controlling HIV-1 replication in vivo	2118:2236	Future studies to evaluate the potential of plant-produced afucosylated PGT121 in controlling HIV-1 replication in vivo are warranted.					
34232070	3	106	theme	large	532:536	arg1	quantities					538:547	the large quantities	528:547	the large quantities of antibodies required for clinical trials	528:590	In addition, different strategies are currently being tested to scale up the production of bNAbs to obtain the large quantities of antibodies required for clinical trials.					
34232070	1	107	theme	cells	265:269	arg1	neutralization					212:225	viral neutralization	206:225	viral neutralization	206:225	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
34232070	1	107	theme	cells	265:269	arg1	elimination					235:245	the elimination	231:245	the elimination of HIV-1-infected cells	231:269	The activity of broadly neutralizing antibodies (bNAbs) targeting HIV-1 depends on pleiotropic functions, including viral neutralization and the elimination of HIV-1-infected cells.					
33068214	7	0	gly	glycopeptides	1501:1513	arg2	glycopeptides					1501:1513	polar-hydrophilic glycopeptides	1483:1513	polar-hydrophilic glycopeptides	1483:1513	Our results revealed that anion-exchange stationary phase is sufficient for glycopeptide enrichment; however, the use of a hydrophobic-containing sorbent was detrimental to the binding of polar-hydrophilic glycopeptides.					
33068214	6	1	theme	O-glycosites	1248:1259	arg1	identification					1230:1243	identification	1230:1243	identification of O-glycosites in densely glycosylated proteins	1230:1292	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	11	2	gly	O-glycopeptide	2146:2159	arg2	O-glycopeptide					2146:2159	O-glycopeptide release	2146:2167	O-glycopeptide release via O-protease	2146:2182	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	4	3	gly	O-glycosite	729:739	arg2	O-glycosite					729:739	O-glycosite mapping	729:747	O-glycosite mapping	729:747	However, O-glycosite mapping remains challenging since mucin-type O-glycans are densely packed, often protecting proteins from cleavage by proteases.					
33068214	9	4	theme	reaction	1788:1795	arg1	time					1797:1800	a longer than normal electron transfer dissociation (ETD) reaction time	1730:1800	a longer than normal electron transfer dissociation (ETD) reaction time	1730:1800	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	3	5	theme	structural	568:577	arg1	characterization					579:594	structural characterization	568:594	structural characterization of glycosylation	568:611	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	4	6	theme	O-glycosite	729:739	arg1	mapping					741:747	O-glycosite mapping	729:747	O-glycosite mapping	729:747	However, O-glycosite mapping remains challenging since mucin-type O-glycans are densely packed, often protecting proteins from cleavage by proteases.					
33068214	10	7	theme	mapping	1913:1919	arg1	strategy					1921:1928	O-glycosite mapping strategy	1901:1928	O-glycosite mapping strategy	1901:1928	O-glycosite mapping strategy via proteases, cut-off filtration and solid-phase chemoenzymatic processing.					
33068214	1	8	theme	post-translational	189:206	arg1	modification					208:219	protein post-translational modification	181:219	protein post-translational modification	181:219	O-glycosylation is a highly diverse and complex form of protein post-translational modification.					
33068214	7	9	theme	polar-hydrophilic	1483:1499	arg1	glycopeptides					1501:1513	polar-hydrophilic glycopeptides	1483:1513	polar-hydrophilic glycopeptides	1483:1513	Our results revealed that anion-exchange stationary phase is sufficient for glycopeptide enrichment; however, the use of a hydrophobic-containing sorbent was detrimental to the binding of polar-hydrophilic glycopeptides.					
33068214	2	10	theme	hydroxyl	320:327	arg1	group					329:333	the hydroxyl group	316:333	the hydroxyl group of serine, threonine and tyrosine residues	316:376	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	10	theme	hydroxyl	320:327	arg1	tyrosine					360:367	tyrosine	360:367	tyrosine	360:367	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	10	theme	hydroxyl	320:327	arg1	threonine					346:354	threonine	346:354	threonine	346:354	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	10	theme	hydroxyl	320:327	arg1	serine					338:343	serine	338:343	serine	338:343	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	6	11	gly	O-glycosites	1248:1259	arg2	O-glycosites					1248:1259	O-glycosites	1248:1259	O-glycosites	1248:1259	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	11	12	theme	O-glycopeptides	2107:2121	arg1	release					2061:2067	release	2061:2067	release of N-glycans	2061:2080	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	11	12	theme	O-glycopeptides	2107:2121	arg1	collection					2083:2092	collection	2083:2092	collection of higher MW O-glycopeptides via MW cut-off filter	2083:2143	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	5	13	theme	given	914:918	arg1	glycosite					920:928	a given glycosite	912:928	a given glycosite	912:928	Adding to the complexity is the fact that a given glycosite can be modified by different glycans, resulting in an array of glycoforms rising from one glycosite.					
33068214	9	14	theme	peptide	1836:1842	arg1	bonds					1844:1848	peptide bonds	1836:1848	peptide bonds	1836:1848	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	6	15	theme	Electron-transfer/Higher	1141:1164	arg1	fragmentation					1204:1216	Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1141:1216	Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1141:1216	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	11	16	theme	higher	2097:2102	arg1	O-glycopeptides					2107:2121	higher MW O-glycopeptides	2097:2121	higher MW O-glycopeptides	2097:2121	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	9	17	gly	O-glycosites	1887:1898	arg2	O-glycosites					1887:1898	O-glycosites	1887:1898	O-glycosites	1887:1898	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	8	18	theme	O-glycosites	1578:1589	arg1	coverage					1566:1573	coverage	1566:1573	coverage of O-glycosites	1566:1589	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	2	19	dep	serine	338:343	arg1	residues					369:376	residues	369:376	residues	369:376	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	20	theme	N-acetyl-galactosamine	281:302	arg1	transfer					269:276	the transfer	265:276	the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues	265:376	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	5	21	gly	glycosite	1020:1028	arg2	glycosite					1020:1028	one glycosite	1016:1028	one glycosite	1016:1028	Adding to the complexity is the fact that a given glycosite can be modified by different glycans, resulting in an array of glycoforms rising from one glycosite.					
33068214	7	22	theme	hydrophobic-containing	1418:1439	arg1	sorbent					1441:1447	a hydrophobic-containing sorbent	1416:1447	a hydrophobic-containing sorbent	1416:1447	Our results revealed that anion-exchange stationary phase is sufficient for glycopeptide enrichment; however, the use of a hydrophobic-containing sorbent was detrimental to the binding of polar-hydrophilic glycopeptides.					
33068214	3	23	from	advanced	631:638	arg1	decade					560:565	the last decade	551:565	the last decade	551:565	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	3	24	theme	analytical	666:675	arg1	methods					677:683	analytical methods	666:683	analytical methods	666:683	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	5	25	theme	different	949:957	arg1	glycans					959:965	different glycans	949:965	different glycans	949:965	Adding to the complexity is the fact that a given glycosite can be modified by different glycans, resulting in an array of glycoforms rising from one glycosite.					
33068214	0	26	theme	chemoenzymatic	80:93	arg1	reaction					95:102	chemoenzymatic reaction	80:102	chemoenzymatic reaction	80:102	Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment.					
33068214	1	27	theme	complex	165:171	arg1	form					173:176	a highly diverse and complex form	144:176	a highly diverse and complex form of protein post-translational modification	144:219	O-glycosylation is a highly diverse and complex form of protein post-translational modification.					
33068214	1	27	theme	complex	165:171	arg1	O-glycosylation					125:139	O-glycosylation	125:139	O-glycosylation	125:139	O-glycosylation is a highly diverse and complex form of protein post-translational modification.					
33068214	8	28	theme	charged	1627:1633	arg1	acids					1641:1645	negatively charged amino acids	1616:1645	negatively charged amino acids	1616:1645	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	7	29	theme	anion-exchange	1321:1334	arg1	phase					1347:1351	anion-exchange stationary phase	1321:1351	anion-exchange stationary phase	1321:1351	Our results revealed that anion-exchange stationary phase is sufficient for glycopeptide enrichment; however, the use of a hydrophobic-containing sorbent was detrimental to the binding of polar-hydrophilic glycopeptides.					
33068214	8	30	theme	acids	1641:1645	arg1	derivatization					1598:1611	derivatization	1598:1611	derivatization of negatively charged amino acids or sialic acids	1598:1661	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	2	31	theme	glycosyltransferases	411:430	arg1	family					401:406	a family	399:406	a family of glycosyltransferases	399:430	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	31	theme	glycosyltransferases	411:430	arg1	N-acetylgalactosaminyltransferases					460:493	the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases	433:493	the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans	433:545	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	0	32	theme	SPE	109:111	arg1	enrichment					113:122	SPE enrichment	109:122	SPE enrichment	109:122	Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment.					
33068214	2	33	theme	E.C.	496:499	arg1	N-acetylgalactosaminyltransferases					460:493	the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases	433:493	the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans	433:545	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	33	theme	E.C.	496:499	arg1	2.4.1.41					501:508	E.C. 2.4.1.41	496:508	E.C. 2.4.1.41	496:508	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	8	34	theme	sialic	1650:1655	arg1	acids					1657:1661	sialic acids	1650:1661	sialic acids	1650:1661	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	5	35	theme	glycoforms	993:1002	arg1	array					984:988	an array	981:988	an array of glycoforms rising from one glycosite	981:1028	Adding to the complexity is the fact that a given glycosite can be modified by different glycans, resulting in an array of glycoforms rising from one glycosite.					
33068214	0	36	theme	LC-MS/MS	16:23	arg1	identification					25:38	online LC-MS/MS identification	9:38	online LC-MS/MS identification of O-glycosites	9:54	Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment.					
33068214	8	37	gly	O-glycopeptides	1707:1721	arg2	O-glycopeptides					1707:1721	a short O-glycopeptides	1699:1721	a short O-glycopeptides	1699:1721	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	3	38	from	development	651:661	arg1	spectrometry					706:717	mass spectrometry	701:717	mass spectrometry	701:717	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	6	39	theme	phase	1082:1086	arg1	SPE					1100:1102	SPE	1100:1102	SPE	1100:1102	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	6	39	theme	phase	1082:1086	arg1	extraction					1088:1097	solid phase extraction	1076:1097	solid phase extraction (SPE) enrichment	1076:1114	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	8	40	theme	Different	1516:1524	arg1	proteases					1526:1534	Different proteases	1516:1534	Different proteases	1516:1534	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	2	41	theme	tyrosine	360:367	arg1	group					329:333	the hydroxyl group	316:333	the hydroxyl group of serine, threonine and tyrosine residues	316:376	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	41	theme	tyrosine	360:367	arg1	tyrosine					360:367	tyrosine	360:367	tyrosine	360:367	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	41	theme	tyrosine	360:367	arg1	threonine					346:354	threonine	346:354	threonine	346:354	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	41	theme	tyrosine	360:367	arg1	serine					338:343	serine	338:343	serine	338:343	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	10	42	theme	chemoenzymatic	1980:1993	arg1	processing					1995:2004	solid-phase chemoenzymatic processing	1968:2004	solid-phase chemoenzymatic processing	1968:2004	O-glycosite mapping strategy via proteases, cut-off filtration and solid-phase chemoenzymatic processing.					
33068214	9	43	theme	longer	1732:1737	arg1	time					1797:1800	a longer than normal electron transfer dissociation (ETD) reaction time	1730:1800	a longer than normal electron transfer dissociation (ETD) reaction time	1730:1800	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	6	44	theme	Dissociation	1183:1194	arg1	fragmentation					1204:1216	Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1141:1216	Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1141:1216	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	8	45	theme	short	1701:1705	arg1	O-glycopeptides					1707:1721	a short O-glycopeptides	1699:1721	a short O-glycopeptides	1699:1721	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	2	46	theme	Mucin-type	222:231	arg1	O-glycosylation					233:247	Mucin-type O-glycosylation	222:247	Mucin-type O-glycosylation	222:247	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	47	theme	threonine	346:354	arg1	group					329:333	the hydroxyl group	316:333	the hydroxyl group of serine, threonine and tyrosine residues	316:376	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	47	theme	threonine	346:354	arg1	tyrosine					360:367	tyrosine	360:367	tyrosine	360:367	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	47	theme	threonine	346:354	arg1	threonine					346:354	threonine	346:354	threonine	346:354	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	47	theme	threonine	346:354	arg1	serine					338:343	serine	338:343	serine	338:343	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	11	48	theme	O-glycopeptide	2146:2159	arg1	release					2161:2167	O-glycopeptide release	2146:2167	O-glycopeptide release via O-protease	2146:2182	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	11	48	theme	O-glycopeptide	2146:2159	arg1	release					2061:2067	release	2061:2067	release of N-glycans	2061:2080	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	0	49	gly	O-glycosites	43:54	arg2	O-glycosites					43:54	O-glycosites	43:54	O-glycosites	43:54	Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment.					
33068214	6	50	theme	Energy	1166:1171	arg1	fragmentation					1204:1216	Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1141:1216	Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1141:1216	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	6	51	gly	glycosylated	1272:1283	arg1	proteins					1285:1292	densely glycosylated proteins	1264:1292	densely glycosylated proteins	1264:1292	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	3	52	theme	last	555:558	arg1	decade					560:565	the last decade	551:565	the last decade	551:565	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	9	53	theme	transfer	1760:1767	arg1	ETD					1783:1785	ETD	1783:1785	ETD	1783:1785	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	9	53	theme	transfer	1760:1767	arg1	dissociation					1769:1780	normal electron transfer dissociation	1744:1780	normal electron transfer dissociation (ETD)	1744:1786	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	10	54	theme	solid-phase	1968:1978	arg1	processing					1995:2004	solid-phase chemoenzymatic processing	1968:2004	solid-phase chemoenzymatic processing	1968:2004	O-glycosite mapping strategy via proteases, cut-off filtration and solid-phase chemoenzymatic processing.					
33068214	5	55	gly	glycosite	920:928	arg2	glycosite					920:928	a given glycosite	912:928	a given glycosite	912:928	Adding to the complexity is the fact that a given glycosite can be modified by different glycans, resulting in an array of glycoforms rising from one glycosite.					
33068214	6	56	theme	digestion	1126:1134	arg1	conditions					1062:1071	conditions	1062:1071	conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1062:1216	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	3	57	from	advances	689:696	arg1	spectrometry					706:717	mass spectrometry	701:717	mass spectrometry	701:717	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	3	58	from	decade	560:565	arg1	advanced					631:638	advanced	631:638	advanced	631:638	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	11	59	theme	MW	2127:2128	arg1	filter					2138:2143	MW cut-off filter	2127:2143	MW cut-off filter	2127:2143	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	2	60	theme	serine	338:343	arg1	group					329:333	the hydroxyl group	316:333	the hydroxyl group of serine, threonine and tyrosine residues	316:376	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	60	theme	serine	338:343	arg1	tyrosine					360:367	tyrosine	360:367	tyrosine	360:367	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	60	theme	serine	338:343	arg1	threonine					346:354	threonine	346:354	threonine	346:354	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	60	theme	serine	338:343	arg1	serine					338:343	serine	338:343	serine	338:343	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	1	61	theme	protein	181:187	arg1	modification					208:219	protein post-translational modification	181:219	protein post-translational modification	181:219	O-glycosylation is a highly diverse and complex form of protein post-translational modification.					
33068214	6	62	theme	fragmentation	1204:1216	arg1	conditions					1062:1071	conditions	1062:1071	conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1062:1216	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	3	63	theme	glycosylation	599:611	arg1	characterization					579:594	structural characterization	568:594	structural characterization of glycosylation	568:611	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	1	64	theme	modification	208:219	arg1	form					173:176	a highly diverse and complex form	144:176	a highly diverse and complex form of protein post-translational modification	144:219	O-glycosylation is a highly diverse and complex form of protein post-translational modification.					
33068214	1	64	theme	modification	208:219	arg1	O-glycosylation					125:139	O-glycosylation	125:139	O-glycosylation	125:139	O-glycosylation is a highly diverse and complex form of protein post-translational modification.					
33068214	9	65	theme	enhanced	1815:1822	arg1	coverage					1824:1831	enhanced coverage	1815:1831	enhanced coverage of peptide bonds that facilitated the localization of O-glycosites	1815:1898	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	6	66	theme	EThcD	1197:1201	arg1	fragmentation					1204:1216	Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1141:1216	Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1141:1216	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	7	67	theme	glycopeptide	1371:1382	arg1	enrichment					1384:1393	glycopeptide enrichment	1371:1393	glycopeptide enrichment	1371:1393	Our results revealed that anion-exchange stationary phase is sufficient for glycopeptide enrichment; however, the use of a hydrophobic-containing sorbent was detrimental to the binding of polar-hydrophilic glycopeptides.					
33068214	10	68	theme	cut-off	1945:1951	arg1	filtration					1953:1962	cut-off filtration	1945:1962	cut-off filtration	1945:1962	O-glycosite mapping strategy via proteases, cut-off filtration and solid-phase chemoenzymatic processing.					
33068214	11	69	theme	cut-off	2130:2136	arg1	filter					2138:2143	MW cut-off filter	2127:2143	MW cut-off filter	2127:2143	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	9	70	theme	bonds	1844:1848	arg1	coverage					1824:1831	enhanced coverage	1815:1831	enhanced coverage of peptide bonds that facilitated the localization of O-glycosites	1815:1898	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	4	71	theme	mucin-type	775:784	arg1	O-glycans					786:794	mucin-type O-glycans	775:794	mucin-type O-glycans	775:794	However, O-glycosite mapping remains challenging since mucin-type O-glycans are densely packed, often protecting proteins from cleavage by proteases.					
33068214	3	72	theme	methods	677:683	arg1	advances					689:696	advances	689:696	advances in mass spectrometry	689:717	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	3	72	theme	methods	677:683	arg1	development					651:661	the development	647:661	the development of analytical methods	647:683	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	10	73	theme	O-glycosite	1901:1911	arg1	strategy					1921:1928	O-glycosite mapping strategy	1901:1928	O-glycosite mapping strategy	1901:1928	O-glycosite mapping strategy via proteases, cut-off filtration and solid-phase chemoenzymatic processing.					
33068214	6	74	theme	solid	1076:1080	arg1	SPE					1100:1102	SPE	1100:1102	SPE	1100:1102	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	6	74	theme	solid	1076:1080	arg1	extraction					1088:1097	solid phase extraction	1076:1097	solid phase extraction (SPE) enrichment	1076:1114	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	6	75	theme	glycosylated	1272:1283	arg1	proteins					1285:1292	densely glycosylated proteins	1264:1292	densely glycosylated proteins	1264:1292	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	8	76	gly	O-glycosites	1578:1589	arg2	O-glycosites					1578:1589	O-glycosites	1578:1589	O-glycosites	1578:1589	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	7	77	theme	sorbent	1441:1447	arg1	use					1409:1411	the use	1405:1411	the use of a hydrophobic-containing sorbent	1405:1447	Our results revealed that anion-exchange stationary phase is sufficient for glycopeptide enrichment; however, the use of a hydrophobic-containing sorbent was detrimental to the binding of polar-hydrophilic glycopeptides.					
33068214	7	77	theme	sorbent	1441:1447	arg1	detrimental					1453:1463	detrimental	1453:1463	detrimental	1453:1463	Our results revealed that anion-exchange stationary phase is sufficient for glycopeptide enrichment; however, the use of a hydrophobic-containing sorbent was detrimental to the binding of polar-hydrophilic glycopeptides.					
33068214	9	78	theme	O-glycosites	1887:1898	arg1	localization					1871:1882	the localization	1867:1882	the localization of O-glycosites	1867:1898	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	8	79	theme	amino	1635:1639	arg1	acids					1641:1645	negatively charged amino acids	1616:1645	negatively charged amino acids	1616:1645	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	9	80	theme	normal	1744:1749	arg1	ETD					1783:1785	ETD	1783:1785	ETD	1783:1785	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	9	80	theme	normal	1744:1749	arg1	dissociation					1769:1780	normal electron transfer dissociation	1744:1780	normal electron transfer dissociation (ETD)	1744:1786	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	7	81	theme	glycopeptides	1501:1513	arg1	binding					1472:1478	the binding	1468:1478	the binding of polar-hydrophilic glycopeptides	1468:1513	Our results revealed that anion-exchange stationary phase is sufficient for glycopeptide enrichment; however, the use of a hydrophobic-containing sorbent was detrimental to the binding of polar-hydrophilic glycopeptides.					
33068214	6	82	from	identification	1230:1243	arg1	proteins					1285:1292	densely glycosylated proteins	1264:1292	densely glycosylated proteins	1264:1292	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	6	83	theme	protease	1117:1124	arg1	digestion					1126:1134	protease digestion	1117:1134	protease digestion	1117:1134	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	8	84	theme	acids	1657:1661	arg1	derivatization					1598:1611	derivatization	1598:1611	derivatization of negatively charged amino acids or sialic acids	1598:1661	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	0	85	theme	online	9:14	arg1	identification					25:38	online LC-MS/MS identification	9:38	online LC-MS/MS identification of O-glycosites	9:54	Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment.					
33068214	6	86	theme	enrichment	1105:1114	arg1	conditions					1062:1071	conditions	1062:1071	conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1062:1216	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	11	87	theme	SPE	2037:2039	arg1	column					2041:2046	SPE column	2037:2046	SPE column	2037:2046	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	11	88	theme	MW	2104:2105	arg1	O-glycopeptides					2107:2121	higher MW O-glycopeptides	2097:2121	higher MW O-glycopeptides	2097:2121	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	2	89	theme	polypeptide	448:458	arg1	N-acetylgalactosaminyltransferases					460:493	the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases	433:493	the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans	433:545	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	89	theme	polypeptide	448:458	arg1	2.4.1.41					501:508	E.C. 2.4.1.41	496:508	E.C. 2.4.1.41	496:508	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	89	theme	polypeptide	448:458	arg1	family					401:406	a family	399:406	a family of glycosyltransferases	399:430	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	3	90	theme	mass	701:704	arg1	spectrometry					706:717	mass spectrometry	701:717	mass spectrometry	701:717	In the last decade, structural characterization of glycosylation has substantially advanced due to the development of analytical methods and advances in mass spectrometry.					
33068214	2	91	theme	UDP-GalNAc	437:446	arg1	N-acetylgalactosaminyltransferases					460:493	the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases	433:493	the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans	433:545	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	91	theme	UDP-GalNAc	437:446	arg1	2.4.1.41					501:508	E.C. 2.4.1.41	496:508	E.C. 2.4.1.41	496:508	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	2	91	theme	UDP-GalNAc	437:446	arg1	family					401:406	a family	399:406	a family of glycosyltransferases	399:430	Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (E.C. 2.4.1.41) that are conserved across metazoans.					
33068214	0	92	theme	O-glycosites	43:54	arg1	identification					25:38	online LC-MS/MS identification	9:38	online LC-MS/MS identification of O-glycosites	9:54	Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment.					
33068214	10	93	gly	O-glycosite	1901:1911	arg2	O-glycosite					1901:1911	O-glycosite	1901:1911	O-glycosite	1901:1911	O-glycosite mapping strategy via proteases, cut-off filtration and solid-phase chemoenzymatic processing.					
33068214	6	94	theme	extraction	1088:1097	arg1	enrichment					1105:1114	solid phase extraction (SPE) enrichment	1076:1114	solid phase extraction (SPE) enrichment	1076:1114	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	5	95	mod	modified	937:944	arg3	glycans					959:965	different glycans	949:965	different glycans	949:965	Adding to the complexity is the fact that a given glycosite can be modified by different glycans, resulting in an array of glycoforms rising from one glycosite.					
33068214	5	95	mod	modified	937:944	arg1	glycosite					920:928	a given glycosite	912:928	a given glycosite	912:928	Adding to the complexity is the fact that a given glycosite can be modified by different glycans, resulting in an array of glycoforms rising from one glycosite.					
33068214	11	96	gly	O-glycopeptides	2107:2121	arg2	O-glycopeptides					2107:2121	higher MW O-glycopeptides	2097:2121	higher MW O-glycopeptides	2097:2121	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	11	97	theme	N-glycans	2072:2080	arg1	release					2161:2167	O-glycopeptide release	2146:2167	O-glycopeptide release via O-protease	2146:2182	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	11	97	theme	N-glycans	2072:2080	arg1	release					2061:2067	release	2061:2067	release of N-glycans	2061:2080	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	11	97	theme	N-glycans	2072:2080	arg1	collection					2083:2092	collection	2083:2092	collection of higher MW O-glycopeptides via MW cut-off filter	2083:2143	Glycopeptides are enriched by SPE column, followed by release of N-glycans, collection of higher MW O-glycopeptides via MW cut-off filter, O-glycopeptide release via O-protease, and finally detected by LC-MS/MS using EThcD.					
33068214	0	98	theme	EThcD	59:63	arg1	fragmentation					65:77	EThcD fragmentation	59:77	EThcD fragmentation	59:77	Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment.					
33068214	7	99	gly	glycopeptide	1371:1382	arg2	glycopeptide					1371:1382	glycopeptide	1371:1382	glycopeptide	1371:1382	Our results revealed that anion-exchange stationary phase is sufficient for glycopeptide enrichment; however, the use of a hydrophobic-containing sorbent was detrimental to the binding of polar-hydrophilic glycopeptides.					
33068214	8	100	theme	O-glycopeptides	1707:1721	arg1	identification					1681:1694	the identification	1677:1694	the identification of a short O-glycopeptides	1677:1721	Different proteases can be employed for enhancing coverage of O-glycosites, while derivatization of negatively charged amino acids or sialic acids would enhance the identification of a short O-glycopeptides.					
33068214	1	101	theme	diverse	153:159	arg1	form					173:176	a highly diverse and complex form	144:176	a highly diverse and complex form of protein post-translational modification	144:219	O-glycosylation is a highly diverse and complex form of protein post-translational modification.					
33068214	1	101	theme	diverse	153:159	arg1	O-glycosylation					125:139	O-glycosylation	125:139	O-glycosylation	125:139	O-glycosylation is a highly diverse and complex form of protein post-translational modification.					
33068214	6	102	theme	Collision	1173:1181	arg1	fragmentation					1204:1216	Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1141:1216	Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation	1141:1216	In this study, we investigated conditions of solid phase extraction (SPE) enrichment, protease digestion, and Electron-transfer/Higher Energy Collision Dissociation (EThcD) fragmentation to optimize identification of O-glycosites in densely glycosylated proteins.					
33068214	9	103	theme	electron	1751:1758	arg1	ETD					1783:1785	ETD	1783:1785	ETD	1783:1785	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	9	103	theme	electron	1751:1758	arg1	dissociation					1769:1780	normal electron transfer dissociation	1744:1780	normal electron transfer dissociation (ETD)	1744:1786	Using a longer than normal electron transfer dissociation (ETD) reaction time, we obtained enhanced coverage of peptide bonds that facilitated the localization of O-glycosites.					
33068214	7	104	theme	stationary	1336:1345	arg1	phase					1347:1351	anion-exchange stationary phase	1321:1351	anion-exchange stationary phase	1321:1351	Our results revealed that anion-exchange stationary phase is sufficient for glycopeptide enrichment; however, the use of a hydrophobic-containing sorbent was detrimental to the binding of polar-hydrophilic glycopeptides.					
32419466	0	0	theme	Wall	89:92	arg1	Particles					94:102	Yeast Cell Wall Particles	78:102	Yeast Cell Wall Particles	78:102	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.					
32419466	0	1	from	Imaging	23:29	arg1	Particles					94:102	Yeast Cell Wall Particles	78:102	Yeast Cell Wall Particles	78:102	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.					
32419466	4	2	theme	particles	762:770	arg1	mapping					741:747	simultaneous chemical, topographical, and mechanical mapping	688:747	simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution	688:842	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	1	3	theme	occurring	161:169	arg1	Particles					105:113	Particles	105:113	Particles extracted from yeast cell walls	105:145	Particles extracted from yeast cell walls are naturally occurring immunomodulators with significant therapeutic applications.					
32419466	1	3	theme	occurring	161:169	arg1	immunomodulators					171:186	naturally occurring immunomodulators	151:186	naturally occurring immunomodulators with significant therapeutic applications	151:228	Particles extracted from yeast cell walls are naturally occurring immunomodulators with significant therapeutic applications.					
32419466	0	4	theme	Cell	84:87	arg1	Particles					94:102	Yeast Cell Wall Particles	78:102	Yeast Cell Wall Particles	78:102	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.					
32419466	1	5	with	immunomodulators	171:186	arg1	applications					217:228	significant therapeutic applications	193:228	significant therapeutic applications	193:228	Particles extracted from yeast cell walls are naturally occurring immunomodulators with significant therapeutic applications.					
32419466	3	6	theme	immune	565:570	arg1	cells					572:576	immune cells	565:576	immune cells	565:576	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	2	7	theme	composition	318:328	arg1	consequence					282:292	a consequence	280:292	a consequence of the overall chemical composition	280:328	Their biological function has been thought to be a consequence of the overall chemical composition.					
32419466	2	7	theme	composition	318:328	arg1	function					248:255	Their biological function	231:255	Their biological function	231:255	Their biological function has been thought to be a consequence of the overall chemical composition.					
32419466	5	8	theme	receptors	1041:1049	arg1	recruitment					1026:1036	heterogeneous recruitment	1012:1036	heterogeneous recruitment of receptors on immune cell membranes	1012:1074	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	4	9	theme	fluorescence	652:663	arg1	microscopy					665:674	super-resolution fluorescence microscopy	635:674	super-resolution fluorescence microscopy	635:674	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	6	10	theme	different	1246:1254	arg1	components					1266:1275	the different cell wall components	1242:1275	the different cell wall components	1242:1275	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	4	11	theme	nm	830:831	arg1	resolution					833:842	≈6 nm resolution	827:842	≈6 nm resolution	827:842	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	2	12	theme	chemical	309:316	arg1	composition					318:328	the overall chemical composition	297:328	the overall chemical composition	297:328	Their biological function has been thought to be a consequence of the overall chemical composition.					
32419466	4	13	theme	super-resolution	635:650	arg1	microscopy					665:674	super-resolution fluorescence microscopy	635:674	super-resolution fluorescence microscopy	635:674	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	3	14	theme	nanoscale	367:375	arg1	visualization					377:389	direct nanoscale visualization	360:389	direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles	360:468	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	5	15	theme	spatial	982:988	arg1	organization					990:1001	their heterogeneous spatial organization	962:1001	their heterogeneous spatial organization	962:1001	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	3	16	gly	heterogeneity	427:439	arg1	particles					460:468	yeast cell wall particles	444:468	yeast cell wall particles	444:468	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	4	17	theme	simultaneous	688:699	arg1	mapping					741:747	simultaneous chemical, topographical, and mechanical mapping	688:747	simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution	688:842	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	2	18	theme	overall	301:307	arg1	composition					318:328	the overall chemical composition	297:328	the overall chemical composition	297:328	Their biological function has been thought to be a consequence of the overall chemical composition.					
32419466	3	19	theme	particles	460:468	arg1	heterogeneity					427:439	the compositional and structural heterogeneity	394:439	the compositional and structural heterogeneity of yeast cell wall particles	394:468	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	6	20	theme	cell	1137:1140	arg1	particles					1147:1155	yeast cell wall particles	1131:1155	yeast cell wall particles	1131:1155	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	0	21	theme	Architectural	47:59	arg1	Heterogeneity					61:73	Chemical and Architectural Heterogeneity	34:73	Chemical and Architectural Heterogeneity	34:73	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.					
32419466	5	22	theme	heterogeneous	968:980	arg1	organization					990:1001	their heterogeneous spatial organization	962:1001	their heterogeneous spatial organization	962:1001	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	3	23	theme	heterogeneity	427:439	arg1	visualization					377:389	direct nanoscale visualization	360:389	direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles	360:468	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	1	24	theme	significant	193:203	arg1	applications					217:228	significant therapeutic applications	193:228	significant therapeutic applications	193:228	Particles extracted from yeast cell walls are naturally occurring immunomodulators with significant therapeutic applications.					
32419466	0	25	theme	Nanoscale	13:21	arg1	Imaging					23:29	Simultaneous Nanoscale Imaging	0:29	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.	0:103	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.					
32419466	5	26	theme	heterogeneous	1012:1024	arg1	recruitment					1026:1036	heterogeneous recruitment	1012:1036	heterogeneous recruitment of receptors on immune cell membranes	1012:1074	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	1	27	theme	therapeutic	205:215	arg1	applications					217:228	significant therapeutic applications	193:228	significant therapeutic applications	193:228	Particles extracted from yeast cell walls are naturally occurring immunomodulators with significant therapeutic applications.					
32419466	0	28	theme	Simultaneous	0:11	arg1	Imaging					23:29	Simultaneous Nanoscale Imaging	0:29	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.	0:103	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.					
32419466	4	29	theme	Saccharomyces	797:809	arg1	cerevisiae					811:820	the yeast Saccharomyces cerevisiae	787:820	the yeast Saccharomyces cerevisiae	787:820	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	4	30	theme	mechanical	730:739	arg1	mapping					741:747	simultaneous chemical, topographical, and mechanical mapping	688:747	simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution	688:842	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	3	31	theme	compositional	398:410	arg1	heterogeneity					427:439	the compositional and structural heterogeneity	394:439	the compositional and structural heterogeneity of yeast cell wall particles	394:468	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	4	32	theme	infrared	603:610	arg1	microscopy					619:628	peak force infrared (PFIR) microscopy	592:628	peak force infrared (PFIR) microscopy	592:628	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	4	33	theme	≈6	827:828	arg1	nm					830:831	nm	830:831	nm	830:831	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	4	34	theme	chemical	701:708	arg1	mapping					741:747	simultaneous chemical, topographical, and mechanical mapping	688:747	simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution	688:842	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	5	35	theme	cell	1061:1064	arg1	membranes					1066:1074	immune cell membranes	1054:1074	immune cell membranes	1054:1074	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	4	36	theme	wall	757:760	arg1	particles					762:770	cell wall particles	752:770	cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution	752:842	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	4	37	theme	cell	752:755	arg1	particles					762:770	cell wall particles	752:770	cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution	752:842	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	3	38	theme	cell	450:453	arg1	particles					460:468	yeast cell wall particles	444:468	yeast cell wall particles	444:468	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	4	39	theme	PFIR	613:616	arg1	microscopy					619:628	peak force infrared (PFIR) microscopy	592:628	peak force infrared (PFIR) microscopy	592:628	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	4	40	theme	topographical	711:723	arg1	mapping					741:747	simultaneous chemical, topographical, and mechanical mapping	688:747	simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution	688:842	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	5	41	from	recruitment	1026:1036	arg1	membranes					1066:1074	immune cell membranes	1054:1074	immune cell membranes	1054:1074	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	3	42	theme	such	491:494	arg1	heterogeneity					506:518	such nanoscale heterogeneity	491:518	such nanoscale heterogeneity	491:518	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	0	43	theme	Chemical	34:41	arg1	Heterogeneity					61:73	Chemical and Architectural Heterogeneity	34:73	Chemical and Architectural Heterogeneity	34:73	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.					
32419466	3	44	theme	wall	455:458	arg1	particles					460:468	yeast cell wall particles	444:468	yeast cell wall particles	444:468	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	6	45	theme	components	1266:1275	arg1	distribution					1226:1237	the nanoscale distribution	1212:1237	not only their overall composition but also the nanoscale distribution of the different cell wall components	1168:1275	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	6	45	theme	components	1266:1275	arg1	composition					1191:1201	their overall composition	1177:1201	not only their overall composition but also the nanoscale distribution of the different cell wall components	1168:1275	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	3	46	theme	nanoscale	496:504	arg1	heterogeneity					506:518	such nanoscale heterogeneity	491:518	such nanoscale heterogeneity	491:518	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	0	47	theme	Heterogeneity	61:73	arg1	Imaging					23:29	Simultaneous Nanoscale Imaging	0:29	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.	0:103	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.					
32419466	5	48	theme	nonuniform	935:944	arg1	structures					946:955	specific nonuniform structures	926:955	specific nonuniform structures	926:955	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	5	49	theme	immune	1054:1059	arg1	membranes					1066:1074	immune cell membranes	1054:1074	immune cell membranes	1054:1074	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	3	50	theme	receptor	544:551	arg1	function					553:560	the receptor function	540:560	the receptor function of immune cells	540:576	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	3	51	theme	yeast	444:448	arg1	particles					460:468	yeast cell wall particles	444:468	yeast cell wall particles	444:468	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	4	52	theme	force	597:601	arg1	microscopy					619:628	peak force infrared (PFIR) microscopy	592:628	peak force infrared (PFIR) microscopy	592:628	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	1	53	theme	yeast	130:134	arg1	walls					141:145	yeast cell walls	130:145	yeast cell walls	130:145	Particles extracted from yeast cell walls are naturally occurring immunomodulators with significant therapeutic applications.					
32419466	4	54	theme	peak	592:595	arg1	microscopy					619:628	peak force infrared (PFIR) microscopy	592:628	peak force infrared (PFIR) microscopy	592:628	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	2	55	theme	biological	237:246	arg1	consequence					282:292	a consequence	280:292	a consequence of the overall chemical composition	280:328	Their biological function has been thought to be a consequence of the overall chemical composition.					
32419466	2	55	theme	biological	237:246	arg1	function					248:255	Their biological function	231:255	Their biological function	231:255	Their biological function has been thought to be a consequence of the overall chemical composition.					
32419466	6	56	theme	biological	1108:1117	arg1	function					1119:1126	the biological function	1104:1126	the biological function of yeast cell wall particles	1104:1155	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	1	57	theme	cell	136:139	arg1	walls					141:145	yeast cell walls	130:145	yeast cell walls	130:145	Particles extracted from yeast cell walls are naturally occurring immunomodulators with significant therapeutic applications.					
32419466	6	58	theme	cell	1256:1259	arg1	components					1266:1275	the different cell wall components	1242:1275	the different cell wall components	1242:1275	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	5	59	dep	polysaccharides	858:872	arg1	polysaccharides					858:872	polysaccharides	858:872	polysaccharides (β-glucan and chitin)	858:894	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	5	59	dep	polysaccharides	858:872	arg1	chitin					888:893	chitin	888:893	chitin	888:893	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	5	59	dep	polysaccharides	858:872	arg1	β-glucan					875:882	β-glucan	875:882	β-glucan	875:882	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	6	60	theme	particles	1147:1155	arg1	function					1119:1126	the biological function	1104:1126	the biological function of yeast cell wall particles	1104:1155	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	6	61	theme	wall	1261:1264	arg1	components					1266:1275	the different cell wall components	1242:1275	the different cell wall components	1242:1275	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	6	62	theme	overall	1183:1189	arg1	composition					1191:1201	their overall composition	1177:1201	not only their overall composition but also the nanoscale distribution of the different cell wall components	1168:1275	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	6	63	theme	nanoscale	1216:1224	arg1	distribution					1226:1237	the nanoscale distribution	1212:1237	not only their overall composition but also the nanoscale distribution of the different cell wall components	1168:1275	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	4	64	theme	yeast	791:795	arg1	cerevisiae					811:820	the yeast Saccharomyces cerevisiae	787:820	the yeast Saccharomyces cerevisiae	787:820	By combining peak force infrared (PFIR) microscopy with super-resolution fluorescence microscopy, we achieve simultaneous chemical, topographical, and mechanical mapping of cell wall particles extracted from the yeast Saccharomyces cerevisiae with ≈6 nm resolution.					
32419466	0	65	theme	Yeast	78:82	arg1	Particles					94:102	Yeast Cell Wall Particles	78:102	Yeast Cell Wall Particles	78:102	Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.					
32419466	6	66	theme	wall	1142:1145	arg1	particles					1147:1155	yeast cell wall particles	1131:1155	yeast cell wall particles	1131:1155	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
32419466	3	67	theme	cells	572:576	arg1	function					553:560	the receptor function	540:560	the receptor function of immune cells	540:576	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	5	68	theme	specific	926:933	arg1	structures					946:955	specific nonuniform structures	926:955	specific nonuniform structures	926:955	We show that polysaccharides (β-glucan and chitin) and proteins are organized in specific nonuniform structures, and their heterogeneous spatial organization leads to heterogeneous recruitment of receptors on immune cell membranes.					
32419466	3	69	theme	direct	360:365	arg1	visualization					377:389	direct nanoscale visualization	360:389	direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles	360:468	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	3	70	theme	structural	416:425	arg1	heterogeneity					427:439	the compositional and structural heterogeneity	394:439	the compositional and structural heterogeneity of yeast cell wall particles	394:468	In contrast, here we achieve direct nanoscale visualization of the compositional and structural heterogeneity of yeast cell wall particles and demonstrate that such nanoscale heterogeneity directly influences the receptor function of immune cells.					
32419466	6	71	theme	yeast	1131:1135	arg1	particles					1147:1155	yeast cell wall particles	1131:1155	yeast cell wall particles	1131:1155	Our findings indicate that the biological function of yeast cell wall particles depends on not only their overall composition but also the nanoscale distribution of the different cell wall components.					
34445285	9	0	from	located	1276:1282	arg1	loop					1316:1319	a highly flexible and mobile loop	1287:1319	a highly flexible and mobile loop of GluA1	1287:1328	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	0	1	from	Mapping	17:23	arg1	Brain					76:80	Mouse Brain	70:80	Mouse Brain	70:80	Peptide Sequence Mapping around Bisecting GlcNAc-Bearing N-Glycans in Mouse Brain.					
34445285	5	2	theme	Mgat3-deficient	643:657	arg1	mice					659:662	Mgat3-deficient mice	643:662	Mgat3-deficient mice	643:662	Here, we performed comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice.					
34445285	8	3	theme	identified	975:984	arg1	sites					1002:1006	identified N-glycosylation sites	975:1006	identified N-glycosylation sites that displayed bisecting GlcNAc	975:1038	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence.					
34445285	9	4	from	GnT-III	1201:1207	arg1	brain					1216:1220	the brain	1212:1220	the brain	1212:1220	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	10	5	theme	modified	1543:1550	arg1	glycans					1552:1558	the modified glycans	1539:1558	the modified glycans	1539:1558	These results suggest that the action of GnT-III is partially affected by the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans.					
34445285	10	6	theme	glycans	1552:1558	arg1	conformation					1523:1534	a bulky flipped-back conformation	1502:1534	a bulky flipped-back conformation of the modified glycans	1502:1558	These results suggest that the action of GnT-III is partially affected by the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans.					
34445285	9	7	theme	mobile	1309:1314	arg1	loop					1316:1319	a highly flexible and mobile loop	1287:1319	a highly flexible and mobile loop of GluA1	1287:1328	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	5	8	theme	mice	659:662	arg1	membranes					611:619	brain membranes	605:619	brain membranes of wild type (WT) and Mgat3-deficient mice	605:662	Here, we performed comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice.					
34445285	2	9	dep	N-acetylglucosaminyltransferase-III	276:310	arg1	GnT-III					313:319	GnT-III	313:319	GnT-III	313:319	A variety of N-glycan structures exist, of which, the formation of bisecting N-acetylglucosamine (GlcNAc) is catalyzed by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene).					
34445285	4	10	theme	target	515:520	arg1	proteins					522:529	the target proteins	511:529	the target proteins	511:529	However, the mechanisms by which GnT-III acts on the target proteins are unknown.					
34445285	6	11	theme	chromatograph-mass	810:827	arg1	spectrometry					829:840	liquid chromatograph-mass spectrometry	803:840	liquid chromatograph-mass spectrometry	803:840	Target glycoproteins of GnT-III were enriched with E4-phytohemagglutinin (PHA) lectin, which recognizes bisecting GlcNAc, and analyzed by liquid chromatograph-mass spectrometry.					
34445285	10	12	theme	proteins	1438:1445	arg1	structure					1418:1426	the tertiary structure	1405:1426	the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans	1405:1558	These results suggest that the action of GnT-III is partially affected by the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans.					
34445285	5	13	theme	wild	624:627	arg1	WT					635:636	WT	635:636	WT	635:636	Here, we performed comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice.					
34445285	5	13	theme	wild	624:627	arg1	type					629:632	wild type	624:632	wild type (WT)	624:637	Here, we performed comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice.					
34445285	9	14	theme	GluA1	1324:1328	arg1	loop					1316:1319	a highly flexible and mobile loop	1287:1319	a highly flexible and mobile loop of GluA1	1287:1328	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	8	15	theme	primary	1093:1099	arg1	sequence					1112:1119	a specific primary amino acid sequence	1082:1119	a specific primary amino acid sequence	1082:1119	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence.					
34445285	5	16	theme	type	629:632	arg1	membranes					611:619	brain membranes	605:619	brain membranes of wild type (WT) and Mgat3-deficient mice	605:662	Here, we performed comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice.					
34445285	6	17	theme	E4-phytohemagglutinin	716:736	arg1	lectin					744:749	E4-phytohemagglutinin (PHA) lectin	716:749	E4-phytohemagglutinin (PHA) lectin	716:749	Target glycoproteins of GnT-III were enriched with E4-phytohemagglutinin (PHA) lectin, which recognizes bisecting GlcNAc, and analyzed by liquid chromatograph-mass spectrometry.					
34445285	8	18	gly	N-glycosylation	986:1000	arg2	sites					1002:1006	identified N-glycosylation sites	975:1006	identified N-glycosylation sites that displayed bisecting GlcNAc	975:1038	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence.					
34445285	8	19	theme	specific	1084:1091	arg1	sequence					1112:1119	a specific primary amino acid sequence	1082:1119	a specific primary amino acid sequence	1082:1119	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence.					
34445285	5	20	theme	glycoproteomic	575:588	arg1	analyses					590:597	comparative glycoproteomic analyses	563:597	comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice	563:662	Here, we performed comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice.					
34445285	9	21	theme	N-glycosylation	1254:1268	arg1	sites					1270:1274	N-glycosylation sites	1254:1274	N-glycosylation sites located in a highly flexible and mobile loop of GluA1	1254:1328	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	3	22	theme	GlcNAc-modified	393:407	arg1	proteins					409:416	various bisecting GlcNAc-modified proteins	375:416	various bisecting GlcNAc-modified proteins involved in Alzheimer's disease and cancer	375:459	We previously identified various bisecting GlcNAc-modified proteins involved in Alzheimer's disease and cancer.					
34445285	8	23	theme	acid	1107:1110	arg1	sequence					1112:1119	a specific primary amino acid sequence	1082:1119	a specific primary amino acid sequence	1082:1119	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence.					
34445285	9	24	theme	molecular	1126:1134	arg1	modeling					1136:1143	The molecular modeling	1122:1143	The molecular modeling of GluA1	1122:1152	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	0	25	theme	Peptide	0:6	arg1	Mapping					17:23	Peptide Sequence Mapping	0:23	Peptide Sequence Mapping around Bisecting GlcNAc-Bearing N-Glycans in Mouse Brain.	0:81	Peptide Sequence Mapping around Bisecting GlcNAc-Bearing N-Glycans in Mouse Brain.					
34445285	8	26	theme	amino	1101:1105	arg1	sequence					1112:1119	a specific primary amino acid sequence	1082:1119	a specific primary amino acid sequence	1082:1119	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence.					
34445285	7	27	theme	bisecting	935:943	arg1	GlcNAc					945:950	bisecting GlcNAc	935:950	bisecting GlcNAc	935:950	We identified 32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc.					
34445285	9	28	gly	N-glycosylation	1254:1268	arg2	sites					1270:1274	N-glycosylation sites	1254:1274	N-glycosylation sites located in a highly flexible and mobile loop of GluA1	1254:1328	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	3	29	theme	various	375:381	arg1	proteins					409:416	various bisecting GlcNAc-modified proteins	375:416	various bisecting GlcNAc-modified proteins involved in Alzheimer's disease and cancer	375:459	We previously identified various bisecting GlcNAc-modified proteins involved in Alzheimer's disease and cancer.					
34445285	10	30	theme	flipped-back	1510:1521	arg1	conformation					1523:1534	a bulky flipped-back conformation	1502:1534	a bulky flipped-back conformation of the modified glycans	1502:1558	These results suggest that the action of GnT-III is partially affected by the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans.					
34445285	5	31	theme	brain	605:609	arg1	membranes					611:619	brain membranes	605:619	brain membranes of wild type (WT) and Mgat3-deficient mice	605:662	Here, we performed comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice.					
34445285	6	32	theme	GnT-III	689:695	arg1	glycoproteins					672:684	Target glycoproteins	665:684	Target glycoproteins of GnT-III	665:695	Target glycoproteins of GnT-III were enriched with E4-phytohemagglutinin (PHA) lectin, which recognizes bisecting GlcNAc, and analyzed by liquid chromatograph-mass spectrometry.					
34445285	10	33	theme	tertiary	1409:1416	arg1	structure					1418:1426	the tertiary structure	1405:1426	the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans	1405:1558	These results suggest that the action of GnT-III is partially affected by the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans.					
34445285	3	34	theme	bisecting	383:391	arg1	proteins					409:416	various bisecting GlcNAc-modified proteins	375:416	various bisecting GlcNAc-modified proteins involved in Alzheimer's disease and cancer	375:459	We previously identified various bisecting GlcNAc-modified proteins involved in Alzheimer's disease and cancer.					
34445285	10	35	theme	bulky	1504:1508	arg1	conformation					1523:1534	a bulky flipped-back conformation	1502:1534	a bulky flipped-back conformation of the modified glycans	1502:1558	These results suggest that the action of GnT-III is partially affected by the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans.					
34445285	1	36	theme	many	116:119	arg1	processes					132:140	many biological processes	116:140	many biological processes in mammals	116:151	N-glycosylation is essential for many biological processes in mammals.					
34445285	9	37	theme	GluA1	1148:1152	arg1	modeling					1136:1143	The molecular modeling	1122:1143	The molecular modeling of GluA1	1122:1152	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	0	38	theme	GlcNAc-Bearing	42:55	arg1	N-Glycans					57:65	Bisecting GlcNAc-Bearing N-Glycans	32:65	Bisecting GlcNAc-Bearing N-Glycans	32:65	Peptide Sequence Mapping around Bisecting GlcNAc-Bearing N-Glycans in Mouse Brain.					
34445285	2	39	theme	N-acetylglucosamine	231:249	arg1	formation					208:216	the formation	204:216	the formation of bisecting N-acetylglucosamine (GlcNAc)	204:258	A variety of N-glycan structures exist, of which, the formation of bisecting N-acetylglucosamine (GlcNAc) is catalyzed by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene).					
34445285	7	40	mod	modified	921:928	arg3	GlcNAc					945:950	bisecting GlcNAc	935:950	bisecting GlcNAc	935:950	We identified 32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc.					
34445285	7	40	mod	modified	921:928	arg1	sites					876:880	32 N-glycosylation sites	857:880	32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc	857:950	We identified 32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc.					
34445285	0	41	theme	Bisecting	32:40	arg1	N-Glycans					57:65	Bisecting GlcNAc-Bearing N-Glycans	32:65	Bisecting GlcNAc-Bearing N-Glycans	32:65	Peptide Sequence Mapping around Bisecting GlcNAc-Bearing N-Glycans in Mouse Brain.					
34445285	8	42	theme	bisecting	1023:1031	arg1	GlcNAc					1033:1038	bisecting GlcNAc	1023:1038	bisecting GlcNAc	1023:1038	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence.					
34445285	9	43	from	loop	1316:1319	arg1	located					1276:1282	located	1276:1282	located	1276:1282	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	2	44	theme	structures	176:185	arg1	variety					156:162	A variety	154:162	A variety of N-glycan structures	154:185	A variety of N-glycan structures exist, of which, the formation of bisecting N-acetylglucosamine (GlcNAc) is catalyzed by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene).					
34445285	2	44	theme	structures	176:185	arg1	structures					176:185	N-glycan structures	167:185	N-glycan structures	167:185	A variety of N-glycan structures exist, of which, the formation of bisecting N-acetylglucosamine (GlcNAc) is catalyzed by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene).					
34445285	2	45	theme	bisecting	221:229	arg1	GlcNAc					252:257	GlcNAc	252:257	GlcNAc	252:257	A variety of N-glycan structures exist, of which, the formation of bisecting N-acetylglucosamine (GlcNAc) is catalyzed by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene).					
34445285	2	45	theme	bisecting	221:229	arg1	N-acetylglucosamine					231:249	bisecting N-acetylglucosamine	221:249	bisecting N-acetylglucosamine (GlcNAc)	221:258	A variety of N-glycan structures exist, of which, the formation of bisecting N-acetylglucosamine (GlcNAc) is catalyzed by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene).					
34445285	7	46	dep	sites	876:880	arg1	Pro					906:908	Pro	906:908	Pro	906:908	We identified 32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc.					
34445285	7	46	dep	sites	876:880	arg1	Xaa					900:902	Xaa	900:902	Xaa	900:902	We identified 32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc.					
34445285	7	46	dep	sites	876:880	arg1	Asn-Xaa-Ser/Thr					883:897	Asn-Xaa-Ser/Thr	883:897	Asn-Xaa-Ser/Thr	883:897	We identified 32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc.					
34445285	10	47	theme	bisecting	1470:1478	arg1	GlcNAc					1480:1485	bisecting GlcNAc	1470:1485	bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans	1470:1558	These results suggest that the action of GnT-III is partially affected by the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans.					
34445285	8	48	theme	sites	1002:1006	arg1	alignment					962:970	Sequence alignment	953:970	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc	953:1038	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence.					
34445285	2	49	theme	N-glycan	167:174	arg1	structures					176:185	N-glycan structures	167:185	N-glycan structures	167:185	A variety of N-glycan structures exist, of which, the formation of bisecting N-acetylglucosamine (GlcNAc) is catalyzed by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene).					
34445285	6	50	theme	bisecting	769:777	arg1	GlcNAc					779:784	bisecting GlcNAc	769:784	bisecting GlcNAc	769:784	Target glycoproteins of GnT-III were enriched with E4-phytohemagglutinin (PHA) lectin, which recognizes bisecting GlcNAc, and analyzed by liquid chromatograph-mass spectrometry.					
34445285	1	51	theme	biological	121:130	arg1	processes					132:140	many biological processes	116:140	many biological processes in mammals	116:151	N-glycosylation is essential for many biological processes in mammals.					
34445285	9	52	theme	flexible	1296:1303	arg1	loop					1316:1319	a highly flexible and mobile loop	1287:1319	a highly flexible and mobile loop of GluA1	1287:1328	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	9	53	theme	located	1276:1282	arg1	sites					1270:1274	N-glycosylation sites	1254:1274	N-glycosylation sites located in a highly flexible and mobile loop of GluA1	1254:1328	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	8	54	theme	Sequence	953:960	arg1	alignment					962:970	Sequence alignment	953:970	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc	953:1038	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence.					
34445285	6	55	gly	glycoproteins	672:684	arg1	glycoproteins					672:684	Target glycoproteins	665:684	Target glycoproteins of GnT-III	665:695	Target glycoproteins of GnT-III were enriched with E4-phytohemagglutinin (PHA) lectin, which recognizes bisecting GlcNAc, and analyzed by liquid chromatograph-mass spectrometry.					
34445285	6	56	theme	Target	665:670	arg1	glycoproteins					672:684	Target glycoproteins	665:684	Target glycoproteins of GnT-III	665:695	Target glycoproteins of GnT-III were enriched with E4-phytohemagglutinin (PHA) lectin, which recognizes bisecting GlcNAc, and analyzed by liquid chromatograph-mass spectrometry.					
34445285	1	57	from	processes	132:140	arg1	mammals					145:151	mammals	145:151	mammals	145:151	N-glycosylation is essential for many biological processes in mammals.					
34445285	10	58	theme	target	1431:1436	arg1	proteins					1438:1445	target proteins	1431:1445	target proteins	1431:1445	These results suggest that the action of GnT-III is partially affected by the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans.					
34445285	5	59	theme	comparative	563:573	arg1	analyses					590:597	comparative glycoproteomic analyses	563:597	comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice	563:662	Here, we performed comparative glycoproteomic analyses using brain membranes of wild type (WT) and Mgat3-deficient mice.					
34445285	2	60	theme	Mgat3	337:341	arg1	gene					343:346	the Mgat3 gene	333:346	the Mgat3 gene	333:346	A variety of N-glycan structures exist, of which, the formation of bisecting N-acetylglucosamine (GlcNAc) is catalyzed by N-acetylglucosaminyltransferase-III (GnT-III, encoded by the Mgat3 gene).					
34445285	9	61	theme	substrates	1186:1195	arg1	one					1157:1159	one	1157:1159	one	1157:1159	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	9	61	theme	substrates	1186:1195	arg1	substrates					1186:1195	the good cell surface substrates	1164:1195	the good cell surface substrates for GnT-III in the brain	1164:1220	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	9	62	theme	good	1168:1171	arg1	substrates					1186:1195	the good cell surface substrates	1164:1195	the good cell surface substrates for GnT-III in the brain	1164:1220	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	9	63	theme	cell	1173:1176	arg1	substrates					1186:1195	the good cell surface substrates	1164:1195	the good cell surface substrates for GnT-III in the brain	1164:1220	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	7	64	theme	N-glycosylation	860:874	arg1	sites					876:880	32 N-glycosylation sites	857:880	32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc	857:950	We identified 32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc.					
34445285	6	65	theme	liquid	803:808	arg1	spectrometry					829:840	liquid chromatograph-mass spectrometry	803:840	liquid chromatograph-mass spectrometry	803:840	Target glycoproteins of GnT-III were enriched with E4-phytohemagglutinin (PHA) lectin, which recognizes bisecting GlcNAc, and analyzed by liquid chromatograph-mass spectrometry.					
34445285	10	66	theme	GnT-III	1372:1378	arg1	action					1362:1367	the action	1358:1367	the action of GnT-III	1358:1378	These results suggest that the action of GnT-III is partially affected by the tertiary structure of target proteins, which can accommodate bisecting GlcNAc that generates a bulky flipped-back conformation of the modified glycans.					
34445285	9	67	theme	surface	1178:1184	arg1	substrates					1186:1195	the good cell surface substrates	1164:1195	the good cell surface substrates for GnT-III in the brain	1164:1220	The molecular modeling of GluA1 as one of the good cell surface substrates for GnT-III in the brain, indicated that GnT-III acts on N-glycosylation sites located in a highly flexible and mobile loop of GluA1.					
34445285	0	68	theme	Mouse	70:74	arg1	Brain					76:80	Mouse Brain	70:80	Mouse Brain	70:80	Peptide Sequence Mapping around Bisecting GlcNAc-Bearing N-Glycans in Mouse Brain.					
34445285	7	69	gly	N-glycosylation	860:874	arg2	sites					876:880	32 N-glycosylation sites	857:880	32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc	857:950	We identified 32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc.					
34445285	7	69	gly	N-glycosylation	860:874	arg2	32					857:858	32	857:858	32	857:858	We identified 32 N-glycosylation sites (Asn-Xaa-Ser/Thr, Xaa ≠ Pro) that were modified with bisecting GlcNAc.					
34445285	8	70	theme	N-glycosylation	986:1000	arg1	sites					1002:1006	identified N-glycosylation sites	975:1006	identified N-glycosylation sites that displayed bisecting GlcNAc	975:1038	Sequence alignment of identified N-glycosylation sites that displayed bisecting GlcNAc suggested that GnT-III does not recognize a specific primary amino acid sequence.					
34445285	0	71	theme	Sequence	8:15	arg1	Mapping					17:23	Peptide Sequence Mapping	0:23	Peptide Sequence Mapping around Bisecting GlcNAc-Bearing N-Glycans in Mouse Brain.	0:81	Peptide Sequence Mapping around Bisecting GlcNAc-Bearing N-Glycans in Mouse Brain.					
32932031	6	0	theme	vaccines	982:989	arg1	development					952:962	the development	948:962	the development of semi-synthetic vaccines against cryptococcal infections	948:1021	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	5	1	theme	no	721:722	arg1	by-products					735:745	no saturation by-products	721:745	no saturation by-products	721:745	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	6	2	theme	semi-synthetic	967:980	arg1	vaccines					982:989	semi-synthetic vaccines	967:989	semi-synthetic vaccines against cryptococcal infections	967:1021	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	5	3	theme	saturation	724:733	arg1	by-products					735:745	no saturation by-products	721:745	no saturation by-products	721:745	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	5	4	theme	global	648:653	arg1	deprotection					655:666	global deprotection	648:666	global deprotection	648:666	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	1	5	theme	frequent	162:169	arg1	pathogen					141:148	an opportunistic fungal pathogen	117:148	an opportunistic fungal pathogen	117:148	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	1	5	theme	frequent	162:169	arg1	neoformans					103:112	Cryptococcus neoformans	90:112	Cryptococcus neoformans	90:112	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	1	5	theme	frequent	162:169	arg1	cause					171:175	a frequent cause	160:175	a frequent cause of a life-threatening meningitis in immunocompromised individuals	160:241	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	3	6	theme	blocks	373:378	arg1	use					335:337	The use	331:337	The use of di- and trisaccharide building blocks	331:378	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
32932031	5	7	theme	catalyst	619:626	arg1	strategy					639:646	our recently disclosed catalyst pre-tuning strategy	596:646	our recently disclosed catalyst pre-tuning strategy	596:646	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	5	8	dep	heptasaccharide	700:714	arg1	yield					774:778	31% yield	770:778	the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield	673:778	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	2	9	theme	heptasaccharide	298:312	arg1	motif					324:328	the serotype B heptasaccharide repeating motif	283:328	the serotype B heptasaccharide repeating motif	283:328	We report the first total synthesis of the serotype B heptasaccharide repeating motif.					
32932031	4	10	theme	1,2-trans	509:517	arg1	selectivity					519:529	total 1,2-trans selectivity	503:529	total 1,2-trans selectivity	503:529	Glycosylations gave total 1,2-trans selectivity, despite the absence of a neighboring participating group.					
32932031	2	11	theme	B	296:296	arg1	motif					324:328	the serotype B heptasaccharide repeating motif	283:328	the serotype B heptasaccharide repeating motif	283:328	We report the first total synthesis of the serotype B heptasaccharide repeating motif.					
32932031	3	12	theme	trisaccharide	350:362	arg1	blocks					373:378	trisaccharide building blocks	350:378	trisaccharide building blocks	350:378	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
32932031	4	13	theme	total	503:507	arg1	selectivity					519:529	total 1,2-trans selectivity	503:529	total 1,2-trans selectivity	503:529	Glycosylations gave total 1,2-trans selectivity, despite the absence of a neighboring participating group.					
32932031	2	14	theme	serotype	287:294	arg1	motif					324:328	the serotype B heptasaccharide repeating motif	283:328	the serotype B heptasaccharide repeating motif	283:328	We report the first total synthesis of the serotype B heptasaccharide repeating motif.					
32932031	3	15	theme	building	364:371	arg1	blocks					373:378	trisaccharide building blocks	350:378	trisaccharide building blocks	350:378	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
32932031	3	16	theme	protected	426:434	arg1	motif					461:465	the protected 6-O-acetylated repeating motif	422:465	the protected 6-O-acetylated repeating motif	422:465	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
32932031	5	17	theme	disclosed	609:617	arg1	strategy					639:646	our recently disclosed catalyst pre-tuning strategy	596:646	our recently disclosed catalyst pre-tuning strategy	596:646	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	0	18	theme	total	11:15	arg1	synthesis					17:25	Convergent total synthesis	0:25	Convergent total synthesis of Cryptococcus neoformans serotype B	0:63	Convergent total synthesis of Cryptococcus neoformans serotype B capsule repeating motif.					
32932031	3	19	from	synthesis	409:417	arg1	steps					476:480	three steps	470:480	three steps	470:480	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
32932031	3	20	theme	di-	342:344	arg1	use					335:337	The use	331:337	The use of di- and trisaccharide building blocks	331:378	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
32932031	1	21	theme	Cryptococcus	90:101	arg1	pathogen					141:148	an opportunistic fungal pathogen	117:148	an opportunistic fungal pathogen	117:148	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	1	21	theme	Cryptococcus	90:101	arg1	neoformans					103:112	Cryptococcus neoformans	90:112	Cryptococcus neoformans	90:112	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	1	21	theme	Cryptococcus	90:101	arg1	cause					171:175	a frequent cause	160:175	a frequent cause of a life-threatening meningitis in immunocompromised individuals	160:241	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	3	22	theme	repeating	451:459	arg1	motif					461:465	the protected 6-O-acetylated repeating motif	422:465	the protected 6-O-acetylated repeating motif	422:465	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
32932031	0	23	theme	Convergent	0:9	arg1	synthesis					17:25	Convergent total synthesis	0:25	Convergent total synthesis of Cryptococcus neoformans serotype B	0:63	Convergent total synthesis of Cryptococcus neoformans serotype B capsule repeating motif.					
32932031	5	24	from	heptasaccharide	700:714	arg1	steps					764:768	four steps	759:768	four steps	759:768	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	5	25	theme	pre-tuning	628:637	arg1	strategy					639:646	our recently disclosed catalyst pre-tuning strategy	596:646	our recently disclosed catalyst pre-tuning strategy	596:646	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	1	26	theme	life-threatening	182:197	arg1	meningitis					199:208	a life-threatening meningitis	180:208	a life-threatening meningitis	180:208	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	5	27	theme	31	770:771	arg1	%					772:772	%	772:772	%	772:772	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	2	28	theme	motif	324:328	arg1	synthesis					270:278	the first total synthesis	254:278	the first total synthesis of the serotype B heptasaccharide repeating motif	254:328	We report the first total synthesis of the serotype B heptasaccharide repeating motif.					
32932031	6	29	theme	structurally	871:882	arg1	diversity					884:892	the structurally diversity	867:892	the structurally diversity of our GXM microarray	867:914	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	1	30	theme	meningitis	199:208	arg1	pathogen					141:148	an opportunistic fungal pathogen	117:148	an opportunistic fungal pathogen	117:148	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	1	30	theme	meningitis	199:208	arg1	neoformans					103:112	Cryptococcus neoformans	90:112	Cryptococcus neoformans	90:112	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	1	30	theme	meningitis	199:208	arg1	cause					171:175	a frequent cause	160:175	a frequent cause of a life-threatening meningitis in immunocompromised individuals	160:241	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	3	31	theme	concise	390:396	arg1	synthesis					409:417	a concise convergent synthesis	388:417	a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps	388:480	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
32932031	5	32	theme	%	772:772	arg1	yield					774:778	31% yield	770:778	the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield	673:778	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	6	33	theme	further	926:932	arg1	steps					934:938	further steps	926:938	further steps towards the development of semi-synthetic vaccines against cryptococcal infections	926:1021	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	2	34	theme	repeating	314:322	arg1	motif					324:328	the serotype B heptasaccharide repeating motif	283:328	the serotype B heptasaccharide repeating motif	283:328	We report the first total synthesis of the serotype B heptasaccharide repeating motif.					
32932031	5	35	with	heptasaccharide	700:714	arg1	by-products					735:745	no saturation by-products	721:745	no saturation by-products	721:745	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	0	36	theme	neoformans	43:52	arg1	synthesis					17:25	Convergent total synthesis	0:25	Convergent total synthesis of Cryptococcus neoformans serotype B	0:63	Convergent total synthesis of Cryptococcus neoformans serotype B capsule repeating motif.					
32932031	3	37	theme	motif	461:465	arg1	synthesis					409:417	a concise convergent synthesis	388:417	a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps	388:480	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
32932031	1	38	theme	immunocompromised	213:229	arg1	individuals					231:241	immunocompromised individuals	213:241	immunocompromised individuals	213:241	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	0	39	theme	Cryptococcus	30:41	arg1	neoformans					43:52	Cryptococcus neoformans	30:52	Cryptococcus neoformans	30:52	Convergent total synthesis of Cryptococcus neoformans serotype B capsule repeating motif.					
32932031	4	40	theme	neighboring	557:567	arg1	group					583:587	a neighboring participating group	555:587	a neighboring participating group	555:587	Glycosylations gave total 1,2-trans selectivity, despite the absence of a neighboring participating group.					
32932031	3	41	theme	6-O-acetylated	436:449	arg1	motif					461:465	the protected 6-O-acetylated repeating motif	422:465	the protected 6-O-acetylated repeating motif	422:465	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
32932031	6	42	theme	cryptococcal	999:1010	arg1	infections					1012:1021	cryptococcal infections	999:1021	cryptococcal infections	999:1021	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	6	43	theme	microarray	905:914	arg1	diversity					884:892	the structurally diversity	867:892	the structurally diversity of our GXM microarray	867:914	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	1	44	theme	opportunistic	120:132	arg1	pathogen					141:148	an opportunistic fungal pathogen	117:148	an opportunistic fungal pathogen	117:148	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	1	44	theme	opportunistic	120:132	arg1	neoformans					103:112	Cryptococcus neoformans	90:112	Cryptococcus neoformans	90:112	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	1	44	theme	opportunistic	120:132	arg1	cause					171:175	a frequent cause	160:175	a frequent cause of a life-threatening meningitis in immunocompromised individuals	160:241	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	0	45	theme	serotype	54:61	arg1	B					63:63	serotype B	54:63	Convergent total synthesis of Cryptococcus neoformans serotype B	0:63	Convergent total synthesis of Cryptococcus neoformans serotype B capsule repeating motif.					
32932031	4	46	theme	group	583:587	arg1	absence					544:550	the absence	540:550	the absence of a neighboring participating group	540:587	Glycosylations gave total 1,2-trans selectivity, despite the absence of a neighboring participating group.					
32932031	6	47	theme	GXM	901:903	arg1	microarray					905:914	our GXM microarray	897:914	our GXM microarray	897:914	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	1	48	theme	fungal	134:139	arg1	pathogen					141:148	an opportunistic fungal pathogen	117:148	an opportunistic fungal pathogen	117:148	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	1	48	theme	fungal	134:139	arg1	neoformans					103:112	Cryptococcus neoformans	90:112	Cryptococcus neoformans	90:112	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	1	48	theme	fungal	134:139	arg1	cause					171:175	a frequent cause	160:175	a frequent cause of a life-threatening meningitis in immunocompromised individuals	160:241	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	5	49	dep	gave	668:671	arg1	Using					590:594	Using	590:594	Using our recently disclosed catalyst pre-tuning strategy	590:646	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	4	50	theme	participating	569:581	arg1	group					583:587	a neighboring participating group	555:587	a neighboring participating group	555:587	Glycosylations gave total 1,2-trans selectivity, despite the absence of a neighboring participating group.					
32932031	6	51	theme	serotype	785:792	arg1	GXM					817:819	GXM	817:819	GXM	817:819	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	6	51	theme	serotype	785:792	arg1	glucuronoxylomannan					796:814	serotype B glucuronoxylomannan	785:814	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study	781:851	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	0	52	dep	synthesis	17:25	arg1	B					63:63	serotype B	54:63	Convergent total synthesis of Cryptococcus neoformans serotype B	0:63	Convergent total synthesis of Cryptococcus neoformans serotype B capsule repeating motif.					
32932031	6	53	theme	glucuronoxylomannan	796:814	arg1	glycans					822:828	The serotype B glucuronoxylomannan (GXM) glycans	781:828	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study	781:851	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	5	54	from	steps	764:768	arg1	heptasaccharide					700:714	heptasaccharide	700:714	heptasaccharide	700:714	Using our recently disclosed catalyst pre-tuning strategy global deprotection gave the desired 6-O-acetylated heptasaccharide with no saturation by-products, overall in four steps 31% yield.					
32932031	0	55	theme	repeating	73:81	arg1	motif					83:87	repeating motif	73:87	repeating motif	73:87	Convergent total synthesis of Cryptococcus neoformans serotype B capsule repeating motif.					
32932031	2	56	theme	total	264:268	arg1	synthesis					270:278	the first total synthesis	254:278	the first total synthesis of the serotype B heptasaccharide repeating motif	254:328	We report the first total synthesis of the serotype B heptasaccharide repeating motif.					
32932031	6	57	theme	B	794:794	arg1	GXM					817:819	GXM	817:819	GXM	817:819	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	6	57	theme	B	794:794	arg1	glucuronoxylomannan					796:814	serotype B glucuronoxylomannan	785:814	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study	781:851	The serotype B glucuronoxylomannan (GXM) glycans accessed in this study will increase the structurally diversity of our GXM microarray, allowing further steps towards the development of semi-synthetic vaccines against cryptococcal infections.					
32932031	2	58	theme	first	258:262	arg1	synthesis					270:278	the first total synthesis	254:278	the first total synthesis of the serotype B heptasaccharide repeating motif	254:328	We report the first total synthesis of the serotype B heptasaccharide repeating motif.					
32932031	1	59	from	cause	171:175	arg1	individuals					231:241	immunocompromised individuals	213:241	immunocompromised individuals	213:241	Cryptococcus neoformans is an opportunistic fungal pathogen, which is a frequent cause of a life-threatening meningitis in immunocompromised individuals.					
32932031	3	60	theme	convergent	398:407	arg1	synthesis					409:417	a concise convergent synthesis	388:417	a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps	388:480	The use of di- and trisaccharide building blocks enabled a concise convergent synthesis of the protected 6-O-acetylated repeating motif in three steps.					
33909026	5	0	theme	luminal	917:923	arg1	domains					973:979	catalytic and lectin domains	952:979	domains	973:979	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	5	0	theme	luminal	917:923	arg1	region					925:930	the luminal region	913:930	the luminal region (i.e., stem region, catalytic and lectin domains)	913:980	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	1	1	theme	Mucin-type	169:178	arg1	O-glycosylation					180:194	Mucin-type O-glycosylation	169:194	Mucin-type O-glycosylation	169:194	Mucin-type O-glycosylation is initiated by the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family of enzymes, which consists of 20 members in humans.					
33909026	1	2	theme	enzymes	286:292	arg1	family					276:281	the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family	212:281	the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family of enzymes	212:292	Mucin-type O-glycosylation is initiated by the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family of enzymes, which consists of 20 members in humans.					
33909026	0	3	theme	luminal	99:105	arg1	regions					107:113	its luminal regions	95:113	its luminal regions interacting with ER resident UGGT1, PLOD3 and LPCAT1	95:166	Polypeptide N-acetylgalactosaminyltransferase 18 retains in endoplasmic reticulum depending on its luminal regions interacting with ER resident UGGT1, PLOD3 and LPCAT1.					
33909026	8	4	theme	ppGalNAc-Ts	1537:1547	arg1	mechanism					1524:1532	a novel subcellular localization mechanism	1491:1532	a novel subcellular localization mechanism of ppGalNAc-Ts	1491:1547	The findings reveal a novel subcellular localization mechanism of ppGalNAc-Ts and provide a foundation to further characterize the function of ppGalNAc-T18 in the ER.					
33909026	2	5	theme	located	372:378	arg1	ppGalNAc-Ts					360:370	other ppGalNAc-Ts	354:370	other ppGalNAc-Ts located in Golgi apparatus	354:397	Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.					
33909026	5	6	theme	other	1145:1149	arg1	ppGalNAc-Ts					1166:1176	other Golgi resident ppGalNAc-Ts	1145:1176	other Golgi resident ppGalNAc-Ts	1145:1176	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	3	7	theme	ppGalNAc-T18	571:582	arg1	localization					587:598	ppGalNAc-T18 ER localization	571:598	ppGalNAc-T18 ER localization	571:598	Here, we report the mechanism for ppGalNAc-T18 ER localization and the function of each structural domain of ppGalNAc-T18.					
33909026	5	8	dep	region	925:930	arg1	absence					902:908	absence	902:908	absence	902:908	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	5	8	dep	region	925:930	arg1	the					898:900	the	898:900	the	898:900	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	4	9	theme	ER	798:799	arg1	localization					801:812	ER localization	798:812	ER localization	798:812	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	8	10	from	function	1602:1609	arg1	ER					1634:1635	the ER	1630:1635	the ER	1630:1635	The findings reveal a novel subcellular localization mechanism of ppGalNAc-Ts and provide a foundation to further characterize the function of ppGalNAc-T18 in the ER.					
33909026	3	11	theme	ER	584:585	arg1	localization					587:598	ppGalNAc-T18 ER localization	571:598	ppGalNAc-T18 ER localization	571:598	Here, we report the mechanism for ppGalNAc-T18 ER localization and the function of each structural domain of ppGalNAc-T18.					
33909026	0	12	theme	resident	135:142	arg1	UGGT1					144:148	ER resident UGGT1	132:148	ER resident UGGT1	132:148	Polypeptide N-acetylgalactosaminyltransferase 18 retains in endoplasmic reticulum depending on its luminal regions interacting with ER resident UGGT1, PLOD3 and LPCAT1.					
33909026	5	13	theme	Golgi	1112:1116	arg1	retention					1118:1126	Golgi retention	1112:1126	Golgi retention	1112:1126	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	1	14	from	members	316:322	arg1	humans					327:332	humans	327:332	humans	327:332	Mucin-type O-glycosylation is initiated by the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family of enzymes, which consists of 20 members in humans.					
33909026	7	15	theme	cytometry	1331:1339	arg1	analysis					1341:1348	flow cytometry analysis	1326:1348	flow cytometry analysis	1326:1348	Furthermore, flow cytometry analysis showed that the entire luminal region is required for the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18.					
33909026	7	16	theme	ppGalNAc-T18	1457:1468	arg1	activity					1445:1452	the non-catalytic O-GalNAc glycosylation activity	1404:1452	the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18	1404:1468	Furthermore, flow cytometry analysis showed that the entire luminal region is required for the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18.					
33909026	0	17	theme	ER	132:133	arg1	UGGT1					144:148	ER resident UGGT1	132:148	ER resident UGGT1	132:148	Polypeptide N-acetylgalactosaminyltransferase 18 retains in endoplasmic reticulum depending on its luminal regions interacting with ER resident UGGT1, PLOD3 and LPCAT1.					
33909026	5	18	theme	conserved	987:995	arg1	RKTK					1019:1022	the conserved Golgi retention motif RKTK	983:1022	the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain	983:1089	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	6	19	from	assays	1214:1219	arg1	Results					1179:1185	Results	1179:1185	Results from coimmunoprecipitation assays	1179:1219	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	4	20	gly	N-glycosylation	845:859	arg1	ppGalNAc-T18					864:875	ppGalNAc-T18	864:875	ppGalNAc-T18	864:875	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	8	21	theme	ppGalNAc-T18	1614:1625	arg1	function					1602:1609	the function	1598:1609	the function of ppGalNAc-T18 in the ER	1598:1635	The findings reveal a novel subcellular localization mechanism of ppGalNAc-Ts and provide a foundation to further characterize the function of ppGalNAc-T18 in the ER.					
33909026	2	22	from	located	372:378	arg1	apparatus					389:397	Golgi apparatus	383:397	Golgi apparatus	383:397	Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.					
33909026	5	23	theme	catalytic	952:960	arg1	domains					973:979	catalytic and lectin domains	952:979	domains	973:979	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	5	23	theme	catalytic	952:960	arg1	region					925:930	the luminal region	913:930	the luminal region (i.e., stem region, catalytic and lectin domains)	913:980	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	0	24	theme	Polypeptide	0:10	arg1	N-acetylgalactosaminyltransferase					12:44	Polypeptide N-acetylgalactosaminyltransferase 18	0:47	Polypeptide N-acetylgalactosaminyltransferase 18	0:47	Polypeptide N-acetylgalactosaminyltransferase 18 retains in endoplasmic reticulum depending on its luminal regions interacting with ER resident UGGT1, PLOD3 and LPCAT1.					
33909026	6	25	theme	resident	1270:1277	arg1	UGGT1					1288:1292	UGGT1	1288:1292	UGGT1	1288:1292	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	6	25	theme	resident	1270:1277	arg1	LPCAT1					1305:1310	LPCAT1	1305:1310	LPCAT1	1305:1310	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	6	25	theme	resident	1270:1277	arg1	proteins					1279:1286	ER resident proteins UGGT1, PLOD3 and LPCAT1	1267:1310	ER resident proteins UGGT1, PLOD3 and LPCAT1	1267:1310	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	6	25	theme	resident	1270:1277	arg1	PLOD3					1295:1299	PLOD3	1295:1299	PLOD3	1295:1299	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	2	26	from	apparatus	389:397	arg1	located					372:378	located	372:378	located	372:378	Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.					
33909026	4	27	theme	ppGalNAc-T18	767:778	arg1	essential					784:792	essential	784:792	essential	784:792	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	27	theme	ppGalNAc-T18	767:778	arg1	ppGalNAc-T18					767:778	ppGalNAc-T18	767:778	ppGalNAc-T18	767:778	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	27	theme	ppGalNAc-T18	767:778	arg1	region					736:741	the luminal stem region	719:741	the luminal stem region	719:741	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	27	theme	ppGalNAc-T18	767:778	arg1	domain					757:762	catalytic domain	747:762	catalytic domain	747:762	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	28	theme	stem	731:734	arg1	essential					784:792	essential	784:792	essential	784:792	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	28	theme	stem	731:734	arg1	region					736:741	the luminal stem region	719:741	the luminal stem region	719:741	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	7	29	theme	entire	1366:1371	arg1	region					1381:1386	the entire luminal region	1362:1386	the entire luminal region	1362:1386	Furthermore, flow cytometry analysis showed that the entire luminal region is required for the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18.					
33909026	5	30	theme	lectin	966:971	arg1	domains					973:979	catalytic and lectin domains	952:979	domains	973:979	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	5	30	theme	lectin	966:971	arg1	region					925:930	the luminal region	913:930	the luminal region (i.e., stem region, catalytic and lectin domains)	913:980	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	6	31	theme	ER	1267:1268	arg1	UGGT1					1288:1292	UGGT1	1288:1292	UGGT1	1288:1292	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	6	31	theme	ER	1267:1268	arg1	LPCAT1					1305:1310	LPCAT1	1305:1310	LPCAT1	1305:1310	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	6	31	theme	ER	1267:1268	arg1	proteins					1279:1286	ER resident proteins UGGT1, PLOD3 and LPCAT1	1267:1310	ER resident proteins UGGT1, PLOD3 and LPCAT1	1267:1310	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	6	31	theme	ER	1267:1268	arg1	PLOD3					1295:1299	PLOD3	1295:1299	PLOD3	1295:1299	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	1	32	theme	polypeptide	216:226	arg1	ppGalNAc-T					264:273	ppGalNAc-T	264:273	ppGalNAc-T	264:273	Mucin-type O-glycosylation is initiated by the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family of enzymes, which consists of 20 members in humans.					
33909026	1	32	theme	polypeptide	216:226	arg1	N-acetylgalactosaminyltransferase					229:261	polypeptide: N-acetylgalactosaminyltransferase	216:261	the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family of enzymes	212:292	Mucin-type O-glycosylation is initiated by the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family of enzymes, which consists of 20 members in humans.					
33909026	8	33	theme	subcellular	1499:1509	arg1	mechanism					1524:1532	a novel subcellular localization mechanism	1491:1532	a novel subcellular localization mechanism of ppGalNAc-Ts	1491:1547	The findings reveal a novel subcellular localization mechanism of ppGalNAc-Ts and provide a foundation to further characterize the function of ppGalNAc-T18 in the ER.					
33909026	2	34	gly	O-glycosylation	520:534	arg1	ER					465:466	ER	465:466	ER	465:466	Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.					
33909026	2	34	gly	O-glycosylation	520:534	arg1	reticulum					454:462	the endoplasmic reticulum	438:462	the endoplasmic reticulum (ER)	438:467	Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.					
33909026	2	35	theme	endoplasmic	442:452	arg1	ER					465:466	ER	465:466	ER	465:466	Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.					
33909026	2	35	theme	endoplasmic	442:452	arg1	reticulum					454:462	the endoplasmic reticulum	438:462	the endoplasmic reticulum (ER)	438:467	Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.					
33909026	0	36	theme	endoplasmic	60:70	arg1	reticulum					72:80	endoplasmic reticulum	60:80	endoplasmic reticulum	60:80	Polypeptide N-acetylgalactosaminyltransferase 18 retains in endoplasmic reticulum depending on its luminal regions interacting with ER resident UGGT1, PLOD3 and LPCAT1.					
33909026	5	37	theme	stem	939:942	arg1	region					944:949	stem region	939:949	stem region	939:949	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	3	38	theme	structural	625:634	arg1	ppGalNAc-T18					646:657	ppGalNAc-T18	646:657	ppGalNAc-T18	646:657	Here, we report the mechanism for ppGalNAc-T18 ER localization and the function of each structural domain of ppGalNAc-T18.					
33909026	3	38	theme	structural	625:634	arg1	domain					636:641	each structural domain	620:641	each structural domain of ppGalNAc-T18	620:657	Here, we report the mechanism for ppGalNAc-T18 ER localization and the function of each structural domain of ppGalNAc-T18.					
33909026	1	39	theme	N-acetylgalactosaminyltransferase	229:261	arg1	family					276:281	the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family	212:281	the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family of enzymes	212:292	Mucin-type O-glycosylation is initiated by the polypeptide: N-acetylgalactosaminyltransferase (ppGalNAc-T) family of enzymes, which consists of 20 members in humans.					
33909026	2	40	theme	ER	501:502	arg1	homeostasis					504:514	ER homeostasis	501:514	ER homeostasis	501:514	Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.					
33909026	5	41	theme	Golgi	1151:1155	arg1	ppGalNAc-Ts					1166:1176	other Golgi resident ppGalNAc-Ts	1145:1176	other Golgi resident ppGalNAc-Ts	1145:1176	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	5	42	theme	ER	1098:1099	arg1	export					1101:1106	ER export	1098:1106	ER export	1098:1106	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	3	43	theme	domain	636:641	arg1	localization					587:598	ppGalNAc-T18 ER localization	571:598	ppGalNAc-T18 ER localization	571:598	Here, we report the mechanism for ppGalNAc-T18 ER localization and the function of each structural domain of ppGalNAc-T18.					
33909026	3	43	theme	domain	636:641	arg1	function					608:615	the function	604:615	the function of each structural domain of ppGalNAc-T18	604:657	Here, we report the mechanism for ppGalNAc-T18 ER localization and the function of each structural domain of ppGalNAc-T18.					
33909026	4	44	theme	truncation	682:691	arg1	mutants					693:699	ppGalNAc-T18 truncation mutants	669:699	ppGalNAc-T18 truncation mutants	669:699	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	5	45	theme	resident	1157:1164	arg1	ppGalNAc-Ts					1166:1176	other Golgi resident ppGalNAc-Ts	1145:1176	other Golgi resident ppGalNAc-Ts	1145:1176	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	5	46	dep	region	944:949	arg1	i.e.					933:936	i.e.	933:936	i.e.	933:936	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	8	47	theme	novel	1493:1497	arg1	mechanism					1524:1532	a novel subcellular localization mechanism	1491:1532	a novel subcellular localization mechanism of ppGalNAc-Ts	1491:1547	The findings reveal a novel subcellular localization mechanism of ppGalNAc-Ts and provide a foundation to further characterize the function of ppGalNAc-T18 in the ER.					
33909026	5	48	theme	cytoplasmic	1035:1045	arg1	tail					1047:1050	the cytoplasmic tail	1031:1050	the cytoplasmic tail combined with the transmembrane domain	1031:1089	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	4	49	theme	catalytic	747:755	arg1	ppGalNAc-T18					767:778	ppGalNAc-T18	767:778	ppGalNAc-T18	767:778	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	49	theme	catalytic	747:755	arg1	domain					757:762	catalytic domain	747:762	catalytic domain	747:762	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	6	50	theme	coimmunoprecipitation	1192:1212	arg1	assays					1214:1219	coimmunoprecipitation assays	1192:1219	coimmunoprecipitation assays	1192:1219	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	4	51	theme	ppGalNAc-T18	864:875	arg1	domain					834:839	the lectin domain	823:839	the lectin domain	823:839	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	51	theme	ppGalNAc-T18	864:875	arg1	ppGalNAc-T18					864:875	ppGalNAc-T18	864:875	ppGalNAc-T18	864:875	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	51	theme	ppGalNAc-T18	864:875	arg1	N-glycosylation					845:859	N-glycosylation	845:859	N-glycosylation of ppGalNAc-T18	845:875	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	2	52	theme	other	354:358	arg1	ppGalNAc-Ts					360:370	other ppGalNAc-Ts	354:370	other ppGalNAc-Ts located in Golgi apparatus	354:397	Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.					
33909026	7	53	theme	luminal	1373:1379	arg1	region					1381:1386	the entire luminal region	1362:1386	the entire luminal region	1362:1386	Furthermore, flow cytometry analysis showed that the entire luminal region is required for the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18.					
33909026	5	54	theme	Golgi	997:1001	arg1	RKTK					1019:1022	the conserved Golgi retention motif RKTK	983:1022	the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain	983:1089	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	4	55	theme	lectin	827:832	arg1	domain					834:839	the lectin domain	823:839	the lectin domain	823:839	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	55	theme	lectin	827:832	arg1	ppGalNAc-T18					864:875	ppGalNAc-T18	864:875	ppGalNAc-T18	864:875	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	7	56	theme	non-catalytic	1408:1420	arg1	activity					1445:1452	the non-catalytic O-GalNAc glycosylation activity	1404:1452	the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18	1404:1468	Furthermore, flow cytometry analysis showed that the entire luminal region is required for the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18.					
33909026	7	57	theme	O-GalNAc	1422:1429	arg1	activity					1445:1452	the non-catalytic O-GalNAc glycosylation activity	1404:1452	the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18	1404:1468	Furthermore, flow cytometry analysis showed that the entire luminal region is required for the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18.					
33909026	7	58	theme	glycosylation	1431:1443	arg1	activity					1445:1452	the non-catalytic O-GalNAc glycosylation activity	1404:1452	the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18	1404:1468	Furthermore, flow cytometry analysis showed that the entire luminal region is required for the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18.					
33909026	6	59	theme	luminal	1237:1243	arg1	region					1245:1250	the luminal region	1233:1250	the luminal region	1233:1250	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	5	60	theme	retention	1003:1011	arg1	RKTK					1019:1022	the conserved Golgi retention motif RKTK	983:1022	the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain	983:1089	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	8	61	theme	localization	1511:1522	arg1	mechanism					1524:1532	a novel subcellular localization mechanism	1491:1532	a novel subcellular localization mechanism of ppGalNAc-Ts	1491:1547	The findings reveal a novel subcellular localization mechanism of ppGalNAc-Ts and provide a foundation to further characterize the function of ppGalNAc-T18 in the ER.					
33909026	6	62	dep	proteins	1279:1286	arg1	UGGT1					1288:1292	UGGT1	1288:1292	UGGT1	1288:1292	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	6	62	dep	proteins	1279:1286	arg1	LPCAT1					1305:1310	LPCAT1	1305:1310	LPCAT1	1305:1310	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	6	62	dep	proteins	1279:1286	arg1	proteins					1279:1286	ER resident proteins UGGT1, PLOD3 and LPCAT1	1267:1310	ER resident proteins UGGT1, PLOD3 and LPCAT1	1267:1310	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	6	62	dep	proteins	1279:1286	arg1	PLOD3					1295:1299	PLOD3	1295:1299	PLOD3	1295:1299	Results from coimmunoprecipitation assays showed that the luminal region interacts with ER resident proteins UGGT1, PLOD3 and LPCAT1.					
33909026	5	63	theme	transmembrane	1070:1082	arg1	domain					1084:1089	the transmembrane domain	1066:1089	the transmembrane domain	1066:1089	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	5	64	theme	motif	1013:1017	arg1	RKTK					1019:1022	the conserved Golgi retention motif RKTK	983:1022	the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain	983:1089	In the absence of the luminal region (i.e., stem region, catalytic and lectin domains), the conserved Golgi retention motif RKTK within the cytoplasmic tail combined with the transmembrane domain ensure ER export and Golgi retention, as observed for other Golgi resident ppGalNAc-Ts.					
33909026	4	65	theme	ppGalNAc-T18	669:680	arg1	mutants					693:699	ppGalNAc-T18 truncation mutants	669:699	ppGalNAc-T18 truncation mutants	669:699	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	66	theme	luminal	723:729	arg1	essential					784:792	essential	784:792	essential	784:792	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	4	66	theme	luminal	723:729	arg1	region					736:741	the luminal stem region	719:741	the luminal stem region	719:741	By using ppGalNAc-T18 truncation mutants, we revealed that the luminal stem region and catalytic domain of ppGalNAc-T18 are essential for ER localization, whereas the lectin domain and N-glycosylation of ppGalNAc-T18 are not required.					
33909026	7	67	theme	flow	1326:1329	arg1	analysis					1341:1348	flow cytometry analysis	1326:1348	flow cytometry analysis	1326:1348	Furthermore, flow cytometry analysis showed that the entire luminal region is required for the non-catalytic O-GalNAc glycosylation activity of ppGalNAc-T18.					
33909026	3	68	theme	ppGalNAc-T18	646:657	arg1	ppGalNAc-T18					646:657	ppGalNAc-T18	646:657	ppGalNAc-T18	646:657	Here, we report the mechanism for ppGalNAc-T18 ER localization and the function of each structural domain of ppGalNAc-T18.					
33909026	3	68	theme	ppGalNAc-T18	646:657	arg1	domain					636:641	each structural domain	620:641	each structural domain of ppGalNAc-T18	620:657	Here, we report the mechanism for ppGalNAc-T18 ER localization and the function of each structural domain of ppGalNAc-T18.					
33909026	2	69	theme	Golgi	383:387	arg1	apparatus					389:397	Golgi apparatus	383:397	Golgi apparatus	383:397	Among them, unlike other ppGalNAc-Ts located in Golgi apparatus, ppGalNAc-T18 distributes primarily in the endoplasmic reticulum (ER) and non-catalytically regulates ER homeostasis and O-glycosylation.					
33810098	0	0	theme	Lacto-N-Tetraose	106:121	arg1	Synthesis					123:131	Lacto-N-Tetraose Synthesis	106:131	Lacto-N-Tetraose Synthesis	106:131	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis.					
33810098	8	1	theme	reported	1416:1423	arg1	variant					1456:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	8	1	theme	reported	1416:1423	arg1	It					1400:1401	It	1400:1401	It	1400:1401	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	6	2	with	enzymes	1135:1141	arg1	yields					1152:1157	LNT yields	1148:1157	LNT yields in the range of 10-30%	1148:1180	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	8	3	theme	transglycosylating	1425:1442	arg1	variant					1456:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	8	3	theme	transglycosylating	1425:1442	arg1	It					1400:1401	It	1400:1401	It	1400:1401	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	6	4	theme	residual	1033:1040	arg1	activity					1052:1059	residual hydrolase activity	1033:1059	residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme	1033:1104	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	8	5	theme	enzymatic	1511:1519	arg1	synthesis					1521:1529	practical enzymatic synthesis	1501:1529	practical enzymatic synthesis of LNT	1501:1536	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	8	6	theme	further	1477:1483	arg1	engineering					1485:1495	further engineering	1477:1495	further engineering for practical enzymatic synthesis of LNT	1477:1536	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	5	7	from	lactose	935:941	arg1	synthesis					877:885	the synthesis	873:885	the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	873:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	6	8	theme	transglycosylating	1116:1133	arg1	enzymes					1135:1141	transglycosylating enzymes	1116:1141	transglycosylating enzymes with LNT yields in the range of 10-30%	1116:1180	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	8	9	theme	practical	1501:1509	arg1	synthesis					1521:1529	practical enzymatic synthesis	1501:1529	practical enzymatic synthesis of LNT	1501:1536	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	6	10	theme	hydrolase	1042:1050	arg1	activity					1052:1059	residual hydrolase activity	1033:1059	residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme	1033:1104	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	7	11	theme	catalytic	1238:1246	arg1	residues					1248:1255	the catalytic residues	1234:1255	the catalytic residues	1234:1255	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	7	12	contain	have	1258:1261	arg2	impact					1271:1276	a large impact	1263:1276	a large impact on the transglycosylation/hydrolysis ratio	1263:1319	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	7	12	contain	have	1258:1261	arg1	Mutations					1183:1191	Mutations	1183:1191	Mutations	1183:1191	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	4	13	from	present	549:555	arg1	microbiota					568:577	the gut microbiota	560:577	the gut microbiota of breast-fed infants	560:599	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	6	14	dep	%	1080:1080	arg1	to					1074:1075	to	1074:1075	to	1074:1075	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	5	15	from	study	722:726	arg1	cleft					775:779	the substrate binding cleft	753:779	the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	753:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	5	15	from	study	722:726	arg1	subsites					741:748	the donor subsites	731:748	the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	731:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	7	16	theme	large	1265:1269	arg1	impact					1271:1276	a large impact	1263:1276	a large impact on the transglycosylation/hydrolysis ratio	1263:1319	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	4	17	dep	lacto-N-tetraose	617:632	arg1	LNT					635:637	LNT	635:637	LNT	635:637	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	5	18	from	β-lacto-N-bioside	913:929	arg1	synthesis					877:885	the synthesis	873:885	the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	873:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	7	19	theme	located	1204:1210	arg1	Mutations					1183:1191	Mutations	1183:1191	Mutations	1183:1191	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	6	20	with	mutants	1020:1026	arg1	activity					1052:1059	residual hydrolase activity	1033:1059	residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme	1033:1104	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	6	21	theme	enzyme	1099:1104	arg1	enzyme					1099:1104	the wild-type enzyme	1085:1104	the wild-type enzyme	1085:1104	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	6	21	theme	enzyme	1099:1104	arg1	%					1080:1080	0.05% to 1.6%	1068:1080	0.05% to 1.6% of the wild-type enzyme	1068:1104	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	4	22	theme	HMOs	688:691	arg1	structure					650:658	the core structure	641:658	the core structure of the most abundant type I HMOs	641:691	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	4	22	theme	HMOs	688:691	arg1	lacto-N-tetraose					617:632	lacto-N-tetraose	617:632	lacto-N-tetraose (LNT)	617:638	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	5	23	theme	substrate	757:765	arg1	cleft					775:779	the substrate binding cleft	753:779	the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	753:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	4	24	theme	GH20	537:540	arg1	Lacto-N-biosidase					478:494	Lacto-N-biosidase	478:494	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB)	478:530	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	4	24	theme	GH20	537:540	arg1	enzyme					542:547	a GH20 enzyme	535:547	a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs	535:691	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	4	25	theme	I	686:686	arg1	HMOs					688:691	the most abundant type I HMOs	663:691	the most abundant type I HMOs	663:691	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	6	26	gly	transglycosylating	1116:1133	arg0	enzymes					1135:1141	transglycosylating enzymes	1116:1141	transglycosylating enzymes with LNT yields in the range of 10-30%	1116:1180	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	8	27	theme	LNT	1534:1536	arg1	synthesis					1521:1529	practical enzymatic synthesis	1501:1529	practical enzymatic synthesis of LNT	1501:1536	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	7	28	from	impact	1271:1276	arg1	ratio					1315:1319	the transglycosylation/hydrolysis ratio	1281:1319	the transglycosylation/hydrolysis ratio	1281:1319	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	6	29	with	enzyme	968:973	arg1	activity					1010:1017	negligible transglycosylation activity	980:1017	negligible transglycosylation activity	980:1017	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	7	30	theme	Trp394	1196:1201	arg1	Mutations					1183:1191	Mutations	1183:1191	Mutations	1183:1191	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	3	31	theme	oligosaccharides	460:475	arg1	synthesis					439:447	the synthesis	435:447	the synthesis of complex oligosaccharides	435:475	Engineering glycoside hydrolases into transglycosylases may provide biocatalytic routes to the synthesis of complex oligosaccharides.					
33810098	0	32	theme	Transglycosylation	0:17	arg1	Activity					19:26	Transglycosylation Activity	0:26	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis	0:131	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis.					
33810098	6	33	theme	wt	965:966	arg1	enzyme					968:973	the wt enzyme	961:973	the wt enzyme with negligible transglycosylation activity	961:1017	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	6	34	theme	%	1180:1180	arg1	range					1166:1170	the range	1162:1170	the range of 10-30%	1162:1180	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	8	35	gly	transglycosylating	1425:1442	arg0	variant					1456:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	8	35	gly	transglycosylating	1425:1442	arg0	It					1400:1401	It	1400:1401	It	1400:1401	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	5	36	theme	p-nitrophenyl	899:911	arg1	β-lacto-N-bioside					913:929	p-nitrophenyl β-lacto-N-bioside	899:929	p-nitrophenyl β-lacto-N-bioside	899:929	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	0	37	theme	Engineered	31:40	arg1	Mutants					76:82	Engineered Bifidobacterium Lacto-N-Biosidase Mutants	31:82	Engineered Bifidobacterium Lacto-N-Biosidase Mutants	31:82	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis.					
33810098	4	38	theme	infants	593:599	arg1	microbiota					568:577	the gut microbiota	560:577	the gut microbiota of breast-fed infants	560:599	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	8	39	theme	enzyme	1449:1454	arg1	variant					1456:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	8	39	theme	enzyme	1449:1454	arg1	It					1400:1401	It	1400:1401	It	1400:1401	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	7	40	theme	%	1391:1391	arg1	yield					1393:1397	32% yield	1389:1397	32% yield	1389:1397	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	3	41	theme	biocatalytic	412:423	arg1	routes					425:430	biocatalytic routes	412:430	biocatalytic routes to the synthesis of complex oligosaccharides	412:475	Engineering glycoside hydrolases into transglycosylases may provide biocatalytic routes to the synthesis of complex oligosaccharides.					
33810098	7	42	from	subsite	1215:1221	arg1	located					1204:1210	located	1204:1210	located	1204:1210	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	7	43	from	located	1204:1210	arg1	subsite					1215:1221	subsite -1	1215:1224	subsite -1	1215:1224	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	1	44	theme	health	138:143	arg1	benefits					145:152	The health benefits	134:152	The health benefits of human milk oligosaccharides (HMOs)	134:190	The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks.					
33810098	6	45	theme	LNT	1148:1150	arg1	yields					1152:1157	LNT yields	1148:1157	LNT yields in the range of 10-30%	1148:1180	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	0	46	theme	Lacto-N-Biosidase	58:74	arg1	Mutants					76:82	Engineered Bifidobacterium Lacto-N-Biosidase Mutants	31:82	Engineered Bifidobacterium Lacto-N-Biosidase Mutants	31:82	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis.					
33810098	6	47	theme	transglycosylation	991:1008	arg1	activity					1010:1017	negligible transglycosylation activity	980:1017	negligible transglycosylation activity	980:1017	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	4	48	from	bifidum	517:523	arg1	Lacto-N-biosidase					478:494	Lacto-N-biosidase	478:494	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB)	478:530	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	4	48	from	bifidum	517:523	arg1	enzyme					542:547	a GH20 enzyme	535:547	a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs	535:691	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	7	49	theme	transglycosylation/hydrolysis	1285:1313	arg1	ratio					1315:1319	the transglycosylation/hydrolysis ratio	1281:1319	the transglycosylation/hydrolysis ratio	1281:1319	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	5	50	theme	donor	735:739	arg1	cleft					775:779	the substrate binding cleft	753:779	the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	753:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	5	50	theme	donor	735:739	arg1	subsites					741:748	the donor subsites	731:748	the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	731:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	6	51	theme	wild-type	1089:1097	arg1	enzyme					1099:1104	the wild-type enzyme	1085:1104	the wild-type enzyme	1085:1104	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	4	52	theme	type	681:684	arg1	HMOs					688:691	the most abundant type I HMOs	663:691	the most abundant type I HMOs	663:691	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	0	53	theme	Bifidobacterium	42:56	arg1	Mutants					76:82	Engineered Bifidobacterium Lacto-N-Biosidase Mutants	31:82	Engineered Bifidobacterium Lacto-N-Biosidase Mutants	31:82	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis.					
33810098	6	54	theme	negligible	980:989	arg1	activity					1010:1017	negligible transglycosylation activity	980:1017	negligible transglycosylation activity	980:1017	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	3	55	theme	complex	452:458	arg1	oligosaccharides					460:475	complex oligosaccharides	452:475	complex oligosaccharides	452:475	Engineering glycoside hydrolases into transglycosylases may provide biocatalytic routes to the synthesis of complex oligosaccharides.					
33810098	0	56	from	Subsites	93:100	arg1	Activity					19:26	Transglycosylation Activity	0:26	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis	0:131	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis.					
33810098	7	57	theme	32	1389:1390	arg1	%					1391:1391	%	1391:1391	%	1391:1391	Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield.					
33810098	1	58	theme	infant	240:245	arg1	milks					255:259	infant formula milks	240:259	infant formula milks	240:259	The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks.					
33810098	4	59	from	microbiota	568:577	arg1	present					549:555	present	549:555	present	549:555	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	4	60	theme	gut	564:566	arg1	microbiota					568:577	the gut microbiota	560:577	the gut microbiota of breast-fed infants	560:599	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	5	61	theme	hydrolytic	806:815	arg1	activity					817:824	hydrolytic activity	806:824	hydrolytic activity	806:824	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	0	62	theme	Donor	87:91	arg1	Subsites					93:100	Donor Subsites	87:100	Donor Subsites for Lacto-N-Tetraose Synthesis	87:131	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis.					
33810098	6	63	from	yields	1152:1157	arg1	range					1166:1170	the range	1162:1170	the range of 10-30%	1162:1180	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	0	64	theme	Mutants	76:82	arg1	Activity					19:26	Transglycosylation Activity	0:26	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis	0:131	Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis.					
33810098	4	65	theme	breast-fed	582:591	arg1	infants					593:599	breast-fed infants	582:599	breast-fed infants	582:599	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	8	66	theme	amenable	1465:1472	arg1	variant					1456:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	8	66	theme	amenable	1465:1472	arg1	It					1400:1401	It	1400:1401	It	1400:1401	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	4	67	attach	present	549:555	arg2	Lacto-N-biosidase					478:494	Lacto-N-biosidase	478:494	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB)	478:530	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	4	67	attach	present	549:555	arg2	enzyme					542:547	a GH20 enzyme	535:547	a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs	535:691	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	4	67	attach	present	549:555	arg1	microbiota					568:577	the gut microbiota	560:577	the gut microbiota of breast-fed infants	560:599	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	5	68	with	cleft	775:779	arg1	aim					790:792	the aim	786:792	the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	786:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	5	69	theme	cleft	775:779	arg1	cleft					775:779	the substrate binding cleft	753:779	the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	753:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	5	69	theme	cleft	775:779	arg1	subsites					741:748	the donor subsites	731:748	the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	731:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	1	70	theme	human	157:161	arg1	HMOs					186:189	HMOs	186:189	HMOs	186:189	The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks.					
33810098	1	70	theme	human	157:161	arg1	oligosaccharides					168:183	human milk oligosaccharides	157:183	human milk oligosaccharides (HMOs)	157:190	The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks.					
33810098	1	71	theme	formula	247:253	arg1	milks					255:259	infant formula milks	240:259	infant formula milks	240:259	The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks.					
33810098	5	72	theme	mutational	711:720	arg1	study					722:726	a mutational study	709:726	a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	709:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	8	73	theme	LnbB	1444:1447	arg1	variant					1456:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	the first reported transglycosylating LnbB enzyme variant	1406:1462	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	8	73	theme	LnbB	1444:1447	arg1	It					1400:1401	It	1400:1401	It	1400:1401	It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.					
33810098	1	74	theme	milk	163:166	arg1	HMOs					186:189	HMOs	186:189	HMOs	186:189	The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks.					
33810098	1	74	theme	milk	163:166	arg1	oligosaccharides					168:183	human milk oligosaccharides	157:183	human milk oligosaccharides (HMOs)	157:190	The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks.					
33810098	6	75	theme	%	1072:1072	arg1	enzyme					1099:1104	the wild-type enzyme	1085:1104	the wild-type enzyme	1085:1104	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	6	75	theme	%	1072:1072	arg1	%					1080:1080	0.05% to 1.6%	1068:1080	0.05% to 1.6% of the wild-type enzyme	1068:1104	As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10-30%.					
33810098	5	76	theme	binding	767:773	arg1	cleft					775:779	the substrate binding cleft	753:779	the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	753:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	4	77	theme	abundant	672:679	arg1	HMOs					688:691	the most abundant type I HMOs	663:691	the most abundant type I HMOs	663:691	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	2	78	theme	HMO	271:273	arg1	synthesis					275:283	HMO synthesis	271:283	HMO synthesis	271:283	However, HMO synthesis is still challenging and only two HMOs have been marketed.					
33810098	1	79	theme	oligosaccharides	168:183	arg1	benefits					145:152	The health benefits	134:152	The health benefits of human milk oligosaccharides (HMOs)	134:190	The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks.					
33810098	5	80	theme	LNT	890:892	arg1	synthesis					877:885	the synthesis	873:885	the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	873:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	3	81	theme	glycoside	356:364	arg1	hydrolases					366:375	glycoside hydrolases	356:375	glycoside hydrolases	356:375	Engineering glycoside hydrolases into transglycosylases may provide biocatalytic routes to the synthesis of complex oligosaccharides.					
33810098	4	82	theme	present	549:555	arg1	Lacto-N-biosidase					478:494	Lacto-N-biosidase	478:494	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB)	478:530	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	4	82	theme	present	549:555	arg1	enzyme					542:547	a GH20 enzyme	535:547	a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs	535:691	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	5	83	theme	transglycosylation	841:858	arg1	activity					860:867	transglycosylation activity	841:867	transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose	841:941	Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose.					
33810098	4	84	theme	core	645:648	arg1	structure					650:658	the core structure	641:658	the core structure of the most abundant type I HMOs	641:691	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
33810098	4	84	theme	core	645:648	arg1	lacto-N-tetraose					617:632	lacto-N-tetraose	617:632	lacto-N-tetraose (LNT)	617:638	Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs.					
32666865	12	0	theme	human	1838:1842	arg1	results					1844:1850	human results	1838:1850	human results highlighting the utility and relevance of this animal model	1838:1910	Posttrauma PGP17(A2G2S1) increase showed overlap with human results highlighting the utility and relevance of this animal model.					
32666865	12	1	theme	A2G2S1	1801:1806	arg1	increase					1809:1816	Posttrauma PGP17(A2G2S1) increase	1784:1816	Posttrauma PGP17(A2G2S1) increase	1784:1816	Posttrauma PGP17(A2G2S1) increase showed overlap with human results highlighting the utility and relevance of this animal model.					
32666865	4	2	theme	electric	787:794	arg1	footshock					796:804	electric footshock	787:804	electric footshock	787:804	We examined male (1) controls, (2) traumatized vulnerable and (3) traumatized resilient rats both before and several weeks after electric footshock.					
32666865	10	3	theme	most	1563:1566	arg1	correlations					1579:1590	most behavioral correlations	1563:1590	most behavioral correlations	1563:1590	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32666865	13	4	theme	glycosylation	1963:1975	arg1	changes					1977:1983	trauma-induced glycosylation changes	1948:1983	trauma-induced glycosylation changes	1948:1983	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	8	5	dep	large	1243:1247	arg1	elaborate					1250:1258	elaborate	1250:1258	elaborate N-glycans	1250:1268	Traumatic stress induced a shift from large, elaborate N-glycans toward simpler neutral structures in the plasma of all traumatized animals and specifically in the prefrontal cortex of vulnerable rats.					
32666865	10	6	from	abundant	1528:1535	arg1	regions					1495:1501	all three brain regions	1479:1501	all three brain regions	1479:1501	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32666865	10	6	from	abundant	1528:1535	arg1	rats					1551:1554	vulnerable rats	1540:1554	vulnerable rats	1540:1554	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32666865	7	7	theme	Innate	1017:1022	arg1	anxiety					1024:1030	Innate anxiety	1017:1030	Innate anxiety	1017:1030	Innate anxiety did not predict vulnerability, but pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2) correlated positively with it, the last one being the most sensitive.					
32666865	3	8	theme	brain	540:544	arg1	hippocampus					574:584	hippocampus	574:584	hippocampus	574:584	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	3	8	theme	brain	540:544	arg1	regions					546:552	three PTSD-related brain regions	521:552	three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP)	521:603	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	3	8	theme	brain	540:544	arg1	cortex					566:571	prefrontal cortex	555:571	prefrontal cortex	555:571	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	3	8	theme	brain	540:544	arg1	amygdala					590:597	amygdala	590:597	amygdala	590:597	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	3	9	dep	cortex	566:571	arg1	BGP					600:602	BGP	600:602	BGP	600:602	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	6	10	theme	Higher	887:892	arg1	behavior					903:910	Higher freezing behavior	887:910	Higher freezing behavior	887:910	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	12	11	theme	model	1906:1910	arg1	relevance					1881:1889	relevance	1881:1889	relevance	1881:1889	Posttrauma PGP17(A2G2S1) increase showed overlap with human results highlighting the utility and relevance of this animal model.					
32666865	12	11	theme	model	1906:1910	arg1	utility					1869:1875	utility	1869:1875	utility	1869:1875	Posttrauma PGP17(A2G2S1) increase showed overlap with human results highlighting the utility and relevance of this animal model.					
32666865	0	12	theme	disorder	117:124	arg1	model					87:91	a rat model	81:91	a rat model of posttraumatic stress disorder	81:124	Searching for glycomic biomarkers for predicting resilience and vulnerability in a rat model of posttraumatic stress disorder.					
32666865	11	13	theme	further	1761:1767	arg1	investigation					1769:1781	further investigation	1761:1781	further investigation	1761:1781	In conclusion, we found N-glycans (especially PGP15(FA3G2)) in plasma as possible biomarkers of vulnerability to trauma that warrants further investigation.					
32666865	11	14	dep	N-glycans	1651:1659	arg1	FA3G2					1679:1683	FA3G2	1679:1683	FA3G2	1679:1683	In conclusion, we found N-glycans (especially PGP15(FA3G2)) in plasma as possible biomarkers of vulnerability to trauma that warrants further investigation.					
32666865	11	14	dep	N-glycans	1651:1659	arg1	PGP15					1673:1677	PGP15	1673:1677	especially PGP15(FA3G2)	1662:1684	In conclusion, we found N-glycans (especially PGP15(FA3G2)) in plasma as possible biomarkers of vulnerability to trauma that warrants further investigation.					
32666865	10	15	theme	prefrontal	1608:1617	arg1	cortex					1619:1624	the prefrontal cortex	1604:1624	the prefrontal cortex	1604:1624	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32666865	8	16	theme	animals	1337:1343	arg1	plasma					1311:1316	the plasma	1307:1316	the plasma of all traumatized animals	1307:1343	Traumatic stress induced a shift from large, elaborate N-glycans toward simpler neutral structures in the plasma of all traumatized animals and specifically in the prefrontal cortex of vulnerable rats.					
32666865	1	17	theme	stress	141:146	arg1	PTSD					158:161	PTSD	158:161	PTSD	158:161	Posttraumatic stress disorder (PTSD) is triggered by traumatic events in 10-20% of exposed subjects.					
32666865	1	17	theme	stress	141:146	arg1	disorder					148:155	Posttraumatic stress disorder	127:155	Posttraumatic stress disorder (PTSD)	127:162	Posttraumatic stress disorder (PTSD) is triggered by traumatic events in 10-20% of exposed subjects.					
32666865	7	18	theme	PGP15	1122:1126	arg1	levels					1077:1082	pretrauma levels	1067:1082	pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2)	1067:1133	Innate anxiety did not predict vulnerability, but pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2) correlated positively with it, the last one being the most sensitive.					
32666865	0	19	theme	glycomic	14:21	arg1	biomarkers					23:32	glycomic biomarkers	14:32	glycomic biomarkers for predicting resilience and vulnerability in a rat model of posttraumatic stress disorder	14:124	Searching for glycomic biomarkers for predicting resilience and vulnerability in a rat model of posttraumatic stress disorder.					
32666865	12	20	dep	utility	1869:1875	arg1	the					1865:1867	the	1865:1867	the	1865:1867	Posttrauma PGP17(A2G2S1) increase showed overlap with human results highlighting the utility and relevance of this animal model.					
32666865	6	21	theme	decreased	916:924	arg1	interest					933:940	decreased social interest	916:940	decreased social interest	916:940	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	8	22	theme	vulnerable	1390:1399	arg1	rats					1401:1404	vulnerable rats	1390:1404	vulnerable rats	1390:1404	Traumatic stress induced a shift from large, elaborate N-glycans toward simpler neutral structures in the plasma of all traumatized animals and specifically in the prefrontal cortex of vulnerable rats.					
32666865	3	23	dep	find	376:379	arg1	2					447:447	2	447:447	2	447:447	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	3	23	dep	find	376:379	arg1	investigate					453:463	investigate	453:463	to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment	450:655	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	3	23	dep	find	376:379	arg1	1					370:370	1	370:370	1	370:370	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	0	24	theme	posttraumatic	96:108	arg1	disorder					117:124	posttraumatic stress disorder	96:124	posttraumatic stress disorder	96:124	Searching for glycomic biomarkers for predicting resilience and vulnerability in a rat model of posttraumatic stress disorder.					
32666865	3	25	dep	affects	476:482	arg1	uncover					613:619	uncover	613:619	uncover specific targets for PTSD treatment	613:655	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	6	26	theme	resilient	1001:1009	arg1	rats					1011:1014	control and resilient rats	989:1014	control and resilient rats	989:1014	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	6	27	dep	detected	947:954	arg1	compared					977:984	compared	977:984	compared to control and resilient rats	977:1014	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	6	28	located	detected	947:954	arg2	behavior					903:910	Higher freezing behavior	887:910	Higher freezing behavior	887:910	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	6	28	located	detected	947:954	arg1	groups					970:975	vulnerable groups	959:975	vulnerable groups	959:975	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	6	28	located	detected	947:954	arg2	interest					933:940	decreased social interest	916:940	decreased social interest	916:940	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	3	29	theme	N-glycome	403:411	arg1	stress-vulnerability					424:443	plasma N-glycome predicting stress-vulnerability	396:443	plasma N-glycome predicting stress-vulnerability	396:443	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	4	30	dep	before	756:761	arg1	both					751:754	both	751:754	both	751:754	We examined male (1) controls, (2) traumatized vulnerable and (3) traumatized resilient rats both before and several weeks after electric footshock.					
32666865	5	31	theme	z-score	857:863	arg1	analysis					865:872	z-score analysis	857:872	z-score analysis of behavior	857:884	Vulnerable and resilient groups were separated by z-score analysis of behavior.					
32666865	6	32	theme	control	989:995	arg1	rats					1011:1014	control and resilient rats	989:1014	control and resilient rats	989:1014	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	11	33	theme	vulnerability	1723:1735	arg1	N-glycans					1651:1659	N-glycans	1651:1659	N-glycans (especially PGP15(FA3G2))	1651:1685	In conclusion, we found N-glycans (especially PGP15(FA3G2)) in plasma as possible biomarkers of vulnerability to trauma that warrants further investigation.					
32666865	11	33	theme	vulnerability	1723:1735	arg1	biomarkers					1709:1718	possible biomarkers	1700:1718	possible biomarkers of vulnerability to trauma that warrants further investigation	1700:1781	In conclusion, we found N-glycans (especially PGP15(FA3G2)) in plasma as possible biomarkers of vulnerability to trauma that warrants further investigation.					
32666865	4	34	dep	rats	746:749	arg1	3					721:721	3	721:721	3	721:721	We examined male (1) controls, (2) traumatized vulnerable and (3) traumatized resilient rats both before and several weeks after electric footshock.					
32666865	7	35	dep	correlated	1135:1144	arg1	one					1175:1177	one	1175:1177	one	1175:1177	Innate anxiety did not predict vulnerability, but pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2) correlated positively with it, the last one being the most sensitive.					
32666865	7	35	dep	correlated	1135:1144	arg1	sensitive					1194:1202	sensitive	1194:1202	sensitive	1194:1202	Innate anxiety did not predict vulnerability, but pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2) correlated positively with it, the last one being the most sensitive.					
32666865	9	36	theme	vulnerable	1456:1465	arg1	animals					1467:1473	vulnerable animals	1456:1473	vulnerable animals	1456:1473	In plasma trauma increased PGP17(A2G2S) level in vulnerable animals.					
32666865	6	37	theme	vulnerable	959:968	arg1	groups					970:975	vulnerable groups	959:975	vulnerable groups	959:975	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	8	38	theme	simpler	1277:1283	arg1	structures					1293:1302	simpler neutral structures	1277:1302	simpler neutral structures	1277:1302	Traumatic stress induced a shift from large, elaborate N-glycans toward simpler neutral structures in the plasma of all traumatized animals and specifically in the prefrontal cortex of vulnerable rats.					
32666865	7	39	theme	pretrauma	1067:1075	arg1	levels					1077:1082	pretrauma levels	1067:1082	pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2)	1067:1133	Innate anxiety did not predict vulnerability, but pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2) correlated positively with it, the last one being the most sensitive.					
32666865	2	40	theme	N-linked	228:235	arg1	glycosylation					237:249	N-linked glycosylation	228:249	N-linked glycosylation	228:249	N-linked glycosylation, by modifying protein functions, may provide an important environmental link predicting vulnerability.					
32666865	3	41	theme	specific	621:628	arg1	targets					630:636	specific targets	621:636	specific targets for PTSD treatment	621:655	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	4	42	theme	vulnerable	705:714	arg1	male					670:673	male (1) controls, (2) traumatized vulnerable	670:714	male	670:673	We examined male (1) controls, (2) traumatized vulnerable and (3) traumatized resilient rats both before and several weeks after electric footshock.					
32666865	13	43	theme	changes	1977:1983	arg1	site					1940:1943	a key site	1934:1943	a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome	1934:2026	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	13	43	theme	changes	1977:1983	arg1	changes					1977:1983	trauma-induced glycosylation changes	1948:1983	trauma-induced glycosylation changes	1948:1983	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	13	43	theme	changes	1977:1983	arg1	cortex					1924:1929	Prefrontal cortex	1913:1929	Prefrontal cortex	1913:1929	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	10	44	theme	F	1510:1510	arg1	BGP11					1504:1508	BGP11	1504:1508	BGP11(F(6)A2B)	1504:1517	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32666865	10	44	theme	F	1510:1510	arg1	A2B					1514:1516	F(6)A2B	1510:1516	F(6)A2B	1510:1516	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32666865	1	45	theme	subjects	218:225	arg1	subjects					218:225	exposed subjects	210:225	exposed subjects	210:225	Posttraumatic stress disorder (PTSD) is triggered by traumatic events in 10-20% of exposed subjects.					
32666865	1	45	theme	subjects	218:225	arg1	%					205:205	10-20%	200:205	10-20% of exposed subjects	200:225	Posttraumatic stress disorder (PTSD) is triggered by traumatic events in 10-20% of exposed subjects.					
32666865	8	46	from	large	1243:1247	arg1	shift					1232:1236	a shift	1230:1236	a shift from large, elaborate N-glycans toward simpler neutral structures	1230:1302	Traumatic stress induced a shift from large, elaborate N-glycans toward simpler neutral structures in the plasma of all traumatized animals and specifically in the prefrontal cortex of vulnerable rats.					
32666865	4	47	theme	several	767:773	arg1	weeks					775:779	several weeks	767:779	several weeks after electric footshock	767:804	We examined male (1) controls, (2) traumatized vulnerable and (3) traumatized resilient rats both before and several weeks after electric footshock.					
32666865	4	48	theme	resilient	736:744	arg1	rats					746:749	traumatized resilient rats	724:749	(3) traumatized resilient rats both before and several weeks after electric footshock	720:804	We examined male (1) controls, (2) traumatized vulnerable and (3) traumatized resilient rats both before and several weeks after electric footshock.					
32666865	10	49	theme	behavioral	1568:1577	arg1	correlations					1579:1590	most behavioral correlations	1563:1590	most behavioral correlations	1563:1590	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32666865	13	50	theme	trauma-induced	1948:1961	arg1	changes					1977:1983	trauma-induced glycosylation changes	1948:1983	trauma-induced glycosylation changes	1948:1983	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	3	51	theme	PTSD-related	527:538	arg1	hippocampus					574:584	hippocampus	574:584	hippocampus	574:584	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	3	51	theme	PTSD-related	527:538	arg1	regions					546:552	three PTSD-related brain regions	521:552	three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP)	521:603	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	3	51	theme	PTSD-related	527:538	arg1	cortex					566:571	prefrontal cortex	555:571	prefrontal cortex	555:571	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	3	51	theme	PTSD-related	527:538	arg1	amygdala					590:597	amygdala	590:597	amygdala	590:597	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	2	52	theme	protein	265:271	arg1	functions					273:281	protein functions	265:281	protein functions	265:281	N-linked glycosylation, by modifying protein functions, may provide an important environmental link predicting vulnerability.					
32666865	2	53	theme	important	299:307	arg1	link					323:326	an important environmental link	296:326	an important environmental link predicting vulnerability	296:351	N-linked glycosylation, by modifying protein functions, may provide an important environmental link predicting vulnerability.					
32666865	6	54	theme	freezing	894:901	arg1	behavior					903:910	Higher freezing behavior	887:910	Higher freezing behavior	887:910	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	10	55	from	rats	1551:1554	arg1	abundant					1528:1535	abundant	1528:1535	abundant	1528:1535	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32666865	12	56	theme	Posttrauma	1784:1793	arg1	increase					1809:1816	Posttrauma PGP17(A2G2S1) increase	1784:1816	Posttrauma PGP17(A2G2S1) increase	1784:1816	Posttrauma PGP17(A2G2S1) increase showed overlap with human results highlighting the utility and relevance of this animal model.					
32666865	12	57	theme	animal	1899:1904	arg1	model					1906:1910	this animal model	1894:1910	this animal model	1894:1910	Posttrauma PGP17(A2G2S1) increase showed overlap with human results highlighting the utility and relevance of this animal model.					
32666865	10	58	theme	brain	1489:1493	arg1	regions					1495:1501	all three brain regions	1479:1501	all three brain regions	1479:1501	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32666865	3	59	theme	prefrontal	555:564	arg1	regions					546:552	three PTSD-related brain regions	521:552	three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP)	521:603	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	3	59	theme	prefrontal	555:564	arg1	cortex					566:571	prefrontal cortex	555:571	prefrontal cortex	555:571	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	1	60	theme	traumatic	180:188	arg1	events					190:195	traumatic events	180:195	traumatic events	180:195	Posttraumatic stress disorder (PTSD) is triggered by traumatic events in 10-20% of exposed subjects.					
32666865	8	61	theme	traumatized	1325:1335	arg1	animals					1337:1343	all traumatized animals	1321:1343	all traumatized animals	1321:1343	Traumatic stress induced a shift from large, elaborate N-glycans toward simpler neutral structures in the plasma of all traumatized animals and specifically in the prefrontal cortex of vulnerable rats.					
32666865	13	62	theme	key	1936:1938	arg1	site					1940:1943	a key site	1934:1943	a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome	1934:2026	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	13	62	theme	key	1936:1938	arg1	changes					1977:1983	trauma-induced glycosylation changes	1948:1983	trauma-induced glycosylation changes	1948:1983	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	13	62	theme	key	1936:1938	arg1	cortex					1924:1929	Prefrontal cortex	1913:1929	Prefrontal cortex	1913:1929	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	5	63	theme	resilient	822:830	arg1	groups					832:837	Vulnerable and resilient groups	807:837	Vulnerable and resilient groups	807:837	Vulnerable and resilient groups were separated by z-score analysis of behavior.					
32666865	1	64	theme	Posttraumatic	127:139	arg1	PTSD					158:161	PTSD	158:161	PTSD	158:161	Posttraumatic stress disorder (PTSD) is triggered by traumatic events in 10-20% of exposed subjects.					
32666865	1	64	theme	Posttraumatic	127:139	arg1	disorder					148:155	Posttraumatic stress disorder	127:155	Posttraumatic stress disorder (PTSD)	127:162	Posttraumatic stress disorder (PTSD) is triggered by traumatic events in 10-20% of exposed subjects.					
32666865	9	65	from	level	1447:1451	arg1	animals					1467:1473	vulnerable animals	1456:1473	vulnerable animals	1456:1473	In plasma trauma increased PGP17(A2G2S) level in vulnerable animals.					
32666865	9	66	theme	plasma	1410:1415	arg1	trauma					1417:1422	plasma trauma	1410:1422	plasma trauma	1410:1422	In plasma trauma increased PGP17(A2G2S) level in vulnerable animals.					
32666865	13	67	theme	Prefrontal	1913:1922	arg1	site					1940:1943	a key site	1934:1943	a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome	1934:2026	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	13	67	theme	Prefrontal	1913:1922	arg1	changes					1977:1983	trauma-induced glycosylation changes	1948:1983	trauma-induced glycosylation changes	1948:1983	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	13	67	theme	Prefrontal	1913:1922	arg1	cortex					1924:1929	Prefrontal cortex	1913:1929	Prefrontal cortex	1913:1929	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	8	68	theme	prefrontal	1369:1378	arg1	cortex					1380:1385	the prefrontal cortex	1365:1385	the prefrontal cortex of vulnerable rats	1365:1404	Traumatic stress induced a shift from large, elaborate N-glycans toward simpler neutral structures in the plasma of all traumatized animals and specifically in the prefrontal cortex of vulnerable rats.					
32666865	0	69	theme	rat	83:85	arg1	model					87:91	a rat model	81:91	a rat model of posttraumatic stress disorder	81:124	Searching for glycomic biomarkers for predicting resilience and vulnerability in a rat model of posttraumatic stress disorder.					
32666865	3	70	from	alterations	381:391	arg1	stress-vulnerability					424:443	plasma N-glycome predicting stress-vulnerability	396:443	plasma N-glycome predicting stress-vulnerability	396:443	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	5	71	theme	Vulnerable	807:816	arg1	groups					832:837	Vulnerable and resilient groups	807:837	Vulnerable and resilient groups	807:837	Vulnerable and resilient groups were separated by z-score analysis of behavior.					
32666865	6	72	theme	social	926:931	arg1	interest					933:940	decreased social interest	916:940	decreased social interest	916:940	Higher freezing behavior and decreased social interest were detected in vulnerable groups compared to control and resilient rats.					
32666865	2	73	link	N-linked	228:235	arg1	glycosylation					237:249	N-linked glycosylation	228:249	N-linked glycosylation	228:249	N-linked glycosylation, by modifying protein functions, may provide an important environmental link predicting vulnerability.					
32666865	5	74	theme	behavior	877:884	arg1	analysis					865:872	z-score analysis	857:872	z-score analysis of behavior	857:884	Vulnerable and resilient groups were separated by z-score analysis of behavior.					
32666865	8	75	theme	rats	1401:1404	arg1	cortex					1380:1385	the prefrontal cortex	1365:1385	the prefrontal cortex of vulnerable rats	1365:1404	Traumatic stress induced a shift from large, elaborate N-glycans toward simpler neutral structures in the plasma of all traumatized animals and specifically in the prefrontal cortex of vulnerable rats.					
32666865	8	76	theme	Traumatic	1205:1213	arg1	stress					1215:1220	Traumatic stress	1205:1220	Traumatic stress	1205:1220	Traumatic stress induced a shift from large, elaborate N-glycans toward simpler neutral structures in the plasma of all traumatized animals and specifically in the prefrontal cortex of vulnerable rats.					
32666865	0	77	theme	stress	110:115	arg1	disorder					117:124	posttraumatic stress disorder	96:124	posttraumatic stress disorder	96:124	Searching for glycomic biomarkers for predicting resilience and vulnerability in a rat model of posttraumatic stress disorder.					
32666865	11	78	theme	possible	1700:1707	arg1	N-glycans					1651:1659	N-glycans	1651:1659	N-glycans (especially PGP15(FA3G2))	1651:1685	In conclusion, we found N-glycans (especially PGP15(FA3G2)) in plasma as possible biomarkers of vulnerability to trauma that warrants further investigation.					
32666865	11	78	theme	possible	1700:1707	arg1	biomarkers					1709:1718	possible biomarkers	1700:1718	possible biomarkers of vulnerability to trauma that warrants further investigation	1700:1781	In conclusion, we found N-glycans (especially PGP15(FA3G2)) in plasma as possible biomarkers of vulnerability to trauma that warrants further investigation.					
32666865	4	79	dep	male	670:673	arg1	controls					679:686	controls	679:686	controls	679:686	We examined male (1) controls, (2) traumatized vulnerable and (3) traumatized resilient rats both before and several weeks after electric footshock.					
32666865	4	79	dep	male	670:673	arg1	1					676:676	1	676:676	1	676:676	We examined male (1) controls, (2) traumatized vulnerable and (3) traumatized resilient rats both before and several weeks after electric footshock.					
32666865	3	80	theme	predicting	413:422	arg1	stress-vulnerability					424:443	plasma N-glycome predicting stress-vulnerability	396:443	plasma N-glycome predicting stress-vulnerability	396:443	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	7	81	theme	PGP10	1087:1091	arg1	levels					1077:1082	pretrauma levels	1067:1082	pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2)	1067:1133	Innate anxiety did not predict vulnerability, but pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2) correlated positively with it, the last one being the most sensitive.					
32666865	3	82	theme	plasma	396:401	arg1	stress-vulnerability					424:443	plasma N-glycome predicting stress-vulnerability	396:443	plasma N-glycome predicting stress-vulnerability	396:443	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	7	83	theme	PGP11	1104:1108	arg1	levels					1077:1082	pretrauma levels	1067:1082	pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2)	1067:1133	Innate anxiety did not predict vulnerability, but pretrauma levels of PGP10(FA1G1Ga1), PGP11(FA2G2), and PGP15(FA3G2) correlated positively with it, the last one being the most sensitive.					
32666865	13	84	theme	behavioral	2009:2018	arg1	outcome					2020:2026	the behavioral outcome	2005:2026	the behavioral outcome	2005:2026	Prefrontal cortex is a key site of trauma-induced glycosylation changes that could modulate the behavioral outcome.					
32666865	10	85	from	regions	1495:1501	arg1	abundant					1528:1535	abundant	1528:1535	abundant	1528:1535	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32666865	4	86	theme	traumatized	724:734	arg1	rats					746:749	traumatized resilient rats	724:749	(3) traumatized resilient rats both before and several weeks after electric footshock	720:804	We examined male (1) controls, (2) traumatized vulnerable and (3) traumatized resilient rats both before and several weeks after electric footshock.					
32666865	9	87	theme	PGP17	1434:1438	arg1	level					1447:1451	PGP17(A2G2S) level	1434:1451	PGP17(A2G2S) level in vulnerable animals	1434:1473	In plasma trauma increased PGP17(A2G2S) level in vulnerable animals.					
32666865	8	88	theme	neutral	1285:1291	arg1	structures					1293:1302	simpler neutral structures	1277:1302	simpler neutral structures	1277:1302	Traumatic stress induced a shift from large, elaborate N-glycans toward simpler neutral structures in the plasma of all traumatized animals and specifically in the prefrontal cortex of vulnerable rats.					
32666865	2	89	theme	environmental	309:321	arg1	link					323:326	an important environmental link	296:326	an important environmental link predicting vulnerability	296:351	N-linked glycosylation, by modifying protein functions, may provide an important environmental link predicting vulnerability.					
32666865	1	90	theme	exposed	210:216	arg1	subjects					218:225	exposed subjects	210:225	exposed subjects	210:225	Posttraumatic stress disorder (PTSD) is triggered by traumatic events in 10-20% of exposed subjects.					
32666865	3	91	theme	PTSD	642:645	arg1	treatment					647:655	PTSD treatment	642:655	PTSD treatment	642:655	Our goals were (1) to find alterations in plasma N-glycome predicting stress-vulnerability; (2) to investigate how trauma affects N-glycome in the plasma (PGP) and in three PTSD-related brain regions (prefrontal cortex, hippocampus and amygdala; BGP), hence, uncover specific targets for PTSD treatment.					
32666865	10	92	theme	vulnerable	1540:1549	arg1	rats					1551:1554	vulnerable rats	1540:1554	vulnerable rats	1540:1554	In all three brain regions, BGP11(F(6)A2B) was more abundant in vulnerable rats, while most behavioral correlations occurred in the prefrontal cortex.					
32569684	2	0	theme	DP	569:570	arg1	CHI-P					576:580	DP 70; CHI-P	569:580	DP 70; CHI-P	569:580	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	2	0	theme	DP	569:570	arg1	polymer					560:566	chitosan polymer	551:566	chitosan polymer (DP 70; CHI-P)	551:581	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	6	1	theme	CHI-OM	1229:1234	arg1	treatments					1236:1245	CHI-OM treatments	1229:1245	CHI-OM treatments	1229:1245	CHI-P treatments increased lignin synthesis more than CHI-OM treatments.					
32569684	8	2	dep	the	1538:1540	arg1	leaves					1542:1547	leaves	1542:1547	leaves	1542:1547	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	7	3	theme	synthesis	1377:1385	arg1	induction					1339:1347	the induction	1335:1347	the induction of cell wall polysaccharide synthesis	1335:1385	However, CHI-OM at 10 mg L-1 concentration was more effective than CHI-P treatments in the induction of cell wall polysaccharide synthesis.					
32569684	6	4	theme	lignin	1202:1207	arg1	synthesis					1209:1217	lignin synthesis	1202:1217	lignin synthesis	1202:1217	CHI-P treatments increased lignin synthesis more than CHI-OM treatments.					
32569684	4	5	theme	lipid	815:819	arg1	structure					821:829	lipid structure	815:829	lipid structure	815:829	CHI-P treatments increased the lipid structure and protein content of the membranes more than CHI-OM treatments.					
32569684	7	6	theme	wall	1357:1360	arg1	synthesis					1377:1385	cell wall polysaccharide synthesis	1352:1385	cell wall polysaccharide synthesis	1352:1385	However, CHI-OM at 10 mg L-1 concentration was more effective than CHI-P treatments in the induction of cell wall polysaccharide synthesis.					
32569684	2	7	from	patterns	648:655	arg1	purpurea					687:694	purpurea	687:694	purpurea	687:694	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	8	8	from	the	1538:1540	arg1	present					1527:1533	present	1527:1533	present	1527:1533	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	1	9	theme	Ipomoea	447:453	arg1	purpurea					455:462	vitro-propagated Ipomoea purpurea	430:462	vitro-propagated Ipomoea purpurea	430:462	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	8	10	from	present	1527:1533	arg1	the					1538:1540	the	1538:1540	the	1538:1540	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	4	11	theme	CHI-OM	878:883	arg1	treatments					885:894	CHI-OM treatments	878:894	CHI-OM treatments	878:894	CHI-P treatments increased the lipid structure and protein content of the membranes more than CHI-OM treatments.					
32569684	5	12	theme	strands	1011:1017	arg1	number					1001:1006	a low number	995:1006	a low number of strands	995:1017	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	6	13	theme	CHI-P	1175:1179	arg1	treatments					1181:1190	CHI-P treatments	1175:1190	CHI-P treatments	1175:1190	CHI-P treatments increased lignin synthesis more than CHI-OM treatments.					
32569684	0	14	from	changes	71:77	arg1	proteins					119:126	proteins	119:126	proteins	119:126	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	0	14	from	changes	71:77	arg1	walls					91:95	cell walls	86:95	cell walls	86:95	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	0	14	from	changes	71:77	arg1	lipids					107:112	membrane lipids	98:112	membrane lipids	98:112	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	2	15	theme	different	638:646	arg1	patterns					648:655	different patterns	638:655	different patterns of biomolecular changes in I. purpurea leaves	638:701	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	8	16	attach	present	1527:1533	arg1	the					1538:1540	the	1538:1540	the	1538:1540	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	8	16	attach	present	1527:1533	arg2	biomolecules					1514:1525	the biomolecules	1510:1525	the biomolecules present in the leaves of I. purpurea	1510:1562	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	1	17	theme	membrane	394:401	arg1	lipids					403:408	membrane lipids	394:408	membrane lipids	394:408	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	0	18	dep	the	131:133	arg1	leaves					135:140	leaves	135:140	leaves	135:140	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	8	19	theme	chitosan	1445:1452	arg1	degree					1435:1440	the polymerization degree	1416:1440	the polymerization degree of chitosan	1416:1452	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	2	20	theme	changes	673:679	arg1	patterns					648:655	different patterns	638:655	different patterns of biomolecular changes in I. purpurea leaves	638:701	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	1	21	theme	structural	303:312	arg1	changes					332:338	structural and compositional changes	303:338	structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy	303:496	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	1	22	theme	-dependent	258:267	arg1	effects					269:275	the polymerization degree (DP) -dependent effects	227:275	the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy	227:496	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	1	23	theme	compositional	318:330	arg1	changes					332:338	structural and compositional changes	303:338	structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy	303:496	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	2	24	theme	chitosan	551:558	arg1	CHI-P					576:580	DP 70; CHI-P	569:580	DP 70; CHI-P	569:580	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	2	24	theme	chitosan	551:558	arg1	polymer					560:566	chitosan polymer	551:566	chitosan polymer (DP 70; CHI-P)	551:581	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	2	25	theme	oligomer	512:519	arg1	DP					530:531	DP	530:531	DP 2-15; CHI-OM	530:544	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	2	25	theme	oligomer	512:519	arg1	mixture					521:527	The chitosan oligomer mixture	499:527	The chitosan oligomer mixture (DP 2-15; CHI-OM)	499:545	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	3	26	theme	membrane	763:770	arg1	structures					772:781	cell membrane structures	758:781	cell membrane structures	758:781	The chitosan variants enhanced the destabilization of cell membrane structures.					
32569684	0	27	theme	membrane	98:105	arg1	lipids					107:112	membrane lipids	98:112	membrane lipids	98:112	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	1	28	theme	cell	351:354	arg1	polysaccharides					361:375	certain cell wall polysaccharides	343:375	certain cell wall polysaccharides (mainly lignin)	343:391	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	0	29	dep	walls	91:95	arg1	the					82:84	the	82:84	the	82:84	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	0	30	theme	Polymerization	0:13	arg1	degree					15:20	Polymerization degree	0:20	Polymerization degree of chitosan	0:32	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	0	31	from	walls	91:95	arg1	the					131:133	the	131:133	the	131:133	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	7	32	theme	10 mg L-1	1267:1275	arg1	concentration					1277:1289	10 mg L-1 concentration	1267:1289	10 mg L-1 concentration	1267:1289	However, CHI-OM at 10 mg L-1 concentration was more effective than CHI-P treatments in the induction of cell wall polysaccharide synthesis.					
32569684	5	33	theme	α-helix	1116:1122	arg1	structures					1124:1133	more extended α-helix structures	1102:1133	more extended α-helix structures	1102:1133	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	8	34	theme	present	1527:1533	arg1	biomolecules					1514:1525	the biomolecules	1510:1525	the biomolecules present in the leaves of I. purpurea	1510:1562	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	2	35	from	purpurea	687:694	arg1	patterns					648:655	different patterns	638:655	different patterns of biomolecular changes in I. purpurea leaves	638:701	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	1	36	from	changes	332:338	arg1	proteins					415:422	proteins	415:422	proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy	415:496	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	1	36	from	changes	332:338	arg1	polysaccharides					361:375	certain cell wall polysaccharides	343:375	certain cell wall polysaccharides (mainly lignin)	343:391	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	1	36	from	changes	332:338	arg1	lipids					403:408	membrane lipids	394:408	membrane lipids	394:408	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	5	37	theme	β-sheet	971:977	arg1	structures					979:988	β-sheet structures	971:988	β-sheet structures with a low number of strands	971:1017	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	4	38	theme	CHI-P	784:788	arg1	treatments					790:799	CHI-P treatments	784:799	CHI-P treatments	784:799	CHI-P treatments increased the lipid structure and protein content of the membranes more than CHI-OM treatments.					
32569684	4	39	dep	structure	821:829	arg1	the					811:813	the	811:813	the	811:813	CHI-P treatments increased the lipid structure and protein content of the membranes more than CHI-OM treatments.					
32569684	1	40	theme	treatments	289:298	arg1	effects					269:275	the polymerization degree (DP) -dependent effects	227:275	the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy	227:496	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	1	41	from	effects	269:275	arg1	changes					332:338	structural and compositional changes	303:338	structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy	303:496	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	2	42	dep	purpurea	687:694	arg1	leaves					696:701	leaves	696:701	leaves	696:701	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	1	43	dep	lignin	385:390	arg1	mainly					378:383	mainly	378:383	mainly	378:383	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	7	44	from	concentration	1277:1289	arg1	CHI-OM					1257:1262	CHI-OM	1257:1262	CHI-OM at 10 mg L-1 concentration	1257:1289	However, CHI-OM at 10 mg L-1 concentration was more effective than CHI-P treatments in the induction of cell wall polysaccharide synthesis.					
32569684	5	45	from	formation	1089:1097	arg1	structure					1152:1160	the secondary structure	1138:1160	the secondary structure of proteins	1138:1172	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	5	46	with	structures	979:988	arg1	number					1001:1006	a low number	995:1006	a low number of strands	995:1017	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	0	47	theme	FT-IR	166:170	arg1	spectroscopy					172:183	An FT-IR spectroscopy	163:183	An FT-IR spectroscopy study	163:189	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	5	48	theme	secondary	1142:1150	arg1	structure					1152:1160	the secondary structure	1138:1160	the secondary structure of proteins	1138:1172	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	4	49	theme	protein	835:841	arg1	content					843:849	protein content	835:849	protein content	835:849	CHI-P treatments increased the lipid structure and protein content of the membranes more than CHI-OM treatments.					
32569684	5	50	contain	have	927:930	arg2	ability					936:942	the ability to induce the formation of β-sheet structures with a low number of strands	932:1017	the ability to induce the formation of β-sheet structures with a low number of strands	932:1017	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	5	50	contain	have	927:930	arg1	treatment					904:912	CHI-OM treatment	897:912	CHI-OM treatment	897:912	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	1	51	theme	vitro-propagated	430:445	arg1	purpurea					455:462	vitro-propagated Ipomoea purpurea	430:462	vitro-propagated Ipomoea purpurea	430:462	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	7	52	theme	polysaccharide	1362:1375	arg1	synthesis					1377:1385	cell wall polysaccharide synthesis	1352:1385	cell wall polysaccharide synthesis	1352:1385	However, CHI-OM at 10 mg L-1 concentration was more effective than CHI-P treatments in the induction of cell wall polysaccharide synthesis.					
32569684	0	53	dep	affects	34:40	arg1	study					185:189	An FT-IR spectroscopy study	163:189	An FT-IR spectroscopy study	163:189	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	7	54	theme	cell	1352:1355	arg1	synthesis					1377:1385	cell wall polysaccharide synthesis	1352:1385	cell wall polysaccharide synthesis	1352:1385	However, CHI-OM at 10 mg L-1 concentration was more effective than CHI-P treatments in the induction of cell wall polysaccharide synthesis.					
32569684	8	55	theme	polymerization	1420:1433	arg1	degree					1435:1440	the polymerization degree	1416:1440	the polymerization degree of chitosan	1416:1452	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	2	56	from	changes	673:679	arg1	purpurea					687:694	purpurea	687:694	purpurea	687:694	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	5	57	theme	CHI-P	1028:1032	arg1	treatment					1034:1042	CHI-P treatment	1028:1042	CHI-P treatment	1028:1042	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	0	58	from	lipids	107:112	arg1	the					131:133	the	131:133	the	131:133	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	3	59	theme	chitosan	708:715	arg1	variants					717:724	The chitosan variants	704:724	The chitosan variants	704:724	The chitosan variants enhanced the destabilization of cell membrane structures.					
32569684	0	60	theme	Ipomoea	145:151	arg1	purpurea					153:160	Ipomoea purpurea	145:160	Ipomoea purpurea	145:160	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	5	61	theme	low	997:999	arg1	number					1001:1006	a low number	995:1006	a low number of strands	995:1017	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	4	62	theme	membranes	858:866	arg1	structure					821:829	lipid structure	815:829	lipid structure	815:829	CHI-P treatments increased the lipid structure and protein content of the membranes more than CHI-OM treatments.					
32569684	4	62	theme	membranes	858:866	arg1	content					843:849	protein content	835:849	protein content	835:849	CHI-P treatments increased the lipid structure and protein content of the membranes more than CHI-OM treatments.					
32569684	7	63	theme	CHI-P	1315:1319	arg1	treatments					1321:1330	CHI-P treatments	1315:1330	CHI-P treatments	1315:1330	However, CHI-OM at 10 mg L-1 concentration was more effective than CHI-P treatments in the induction of cell wall polysaccharide synthesis.					
32569684	3	64	theme	cell	758:761	arg1	structures					772:781	cell membrane structures	758:781	cell membrane structures	758:781	The chitosan variants enhanced the destabilization of cell membrane structures.					
32569684	2	65	theme	biomolecular	660:671	arg1	changes					673:679	biomolecular changes	660:679	biomolecular changes in I. purpurea leaves	660:701	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	5	66	dep	found	918:922	arg1	whereas					1020:1026	whereas	1020:1026	whereas	1020:1026	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	0	67	theme	cell	86:89	arg1	walls					91:95	cell walls	86:95	cell walls	86:95	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	5	68	contain	have	1057:1060	arg1	treatment					1034:1042	CHI-P treatment	1028:1042	CHI-P treatment	1028:1042	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	5	68	contain	have	1057:1060	arg2	ability					1066:1072	the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins	1062:1172	the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins	1062:1172	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	0	69	theme	spectroscopy	172:183	arg1	study					185:189	An FT-IR spectroscopy study	163:189	An FT-IR spectroscopy study	163:189	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	2	70	theme	chitosan	503:510	arg1	DP					530:531	DP	530:531	DP 2-15; CHI-OM	530:544	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	2	70	theme	chitosan	503:510	arg1	mixture					521:527	The chitosan oligomer mixture	499:527	The chitosan oligomer mixture (DP 2-15; CHI-OM)	499:545	The chitosan oligomer mixture (DP 2-15; CHI-OM) and chitosan polymer (DP 70; CHI-P) applied at 5, 10, and 20 mg L-1 concentrations induced different patterns of biomolecular changes in I. purpurea leaves.					
32569684	3	71	theme	structures	772:781	arg1	destabilization					739:753	the destabilization	735:753	the destabilization of cell membrane structures	735:781	The chitosan variants enhanced the destabilization of cell membrane structures.					
32569684	1	72	theme	certain	343:349	arg1	polysaccharides					361:375	certain cell wall polysaccharides	343:375	certain cell wall polysaccharides (mainly lignin)	343:391	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	7	73	from	effective	1300:1308	arg1	induction					1339:1347	the induction	1335:1347	the induction of cell wall polysaccharide synthesis	1335:1385	However, CHI-OM at 10 mg L-1 concentration was more effective than CHI-P treatments in the induction of cell wall polysaccharide synthesis.					
32569684	0	74	from	proteins	119:126	arg1	the					131:133	the	131:133	the	131:133	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	1	75	theme	wall	356:359	arg1	polysaccharides					361:375	certain cell wall polysaccharides	343:375	certain cell wall polysaccharides (mainly lignin)	343:391	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	5	76	theme	CHI-OM	897:902	arg1	treatment					904:912	CHI-OM treatment	897:912	CHI-OM treatment	897:912	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	8	77	theme	biomolecules	1514:1525	arg1	compositions					1494:1505	compositions	1494:1505	compositions	1494:1505	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	8	77	theme	biomolecules	1514:1525	arg1	structures					1479:1488	structures	1479:1488	structures	1479:1488	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	0	78	theme	chitosan	25:32	arg1	degree					15:20	Polymerization degree	0:20	Polymerization degree of chitosan	0:32	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	5	79	theme	extended	1107:1114	arg1	structures					1124:1133	more extended α-helix structures	1102:1133	more extended α-helix structures	1102:1133	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	0	80	theme	structural	42:51	arg1	changes					71:77	structural and compositional changes	42:77	structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea	42:160	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	5	81	theme	structures	1124:1133	arg1	formation					1089:1097	the formation	1085:1097	the formation of more extended α-helix structures in the secondary structure of proteins	1085:1172	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	7	82	from	induction	1339:1347	arg1	effective					1300:1308	effective	1300:1308	effective	1300:1308	However, CHI-OM at 10 mg L-1 concentration was more effective than CHI-P treatments in the induction of cell wall polysaccharide synthesis.					
32569684	0	83	theme	compositional	57:69	arg1	changes					71:77	structural and compositional changes	42:77	structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea	42:160	Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study.					
32569684	1	84	theme	chitosan	280:287	arg1	treatments					289:298	chitosan treatments	280:298	chitosan treatments	280:298	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	5	85	theme	structures	979:988	arg1	formation					958:966	the formation	954:966	the formation of β-sheet structures with a low number of strands	954:1017	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	8	86	theme	I.	1552:1553	arg1	purpurea					1555:1562	I. purpurea	1552:1562	I. purpurea	1552:1562	These findings suggest that the polymerization degree of chitosan plays a role in changing structures and compositions of the biomolecules present in the leaves of I. purpurea.					
32569684	1	87	theme	FT-IR	479:483	arg1	spectroscopy					485:496	FT-IR spectroscopy	479:496	FT-IR spectroscopy	479:496	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32569684	5	88	theme	proteins	1165:1172	arg1	structure					1152:1160	the secondary structure	1138:1160	the secondary structure of proteins	1138:1172	CHI-OM treatment was found to have the ability to induce the formation of β-sheet structures with a low number of strands, whereas CHI-P treatment was found to have the ability to trigger the formation of more extended α-helix structures in the secondary structure of proteins.					
32569684	1	89	dep	polysaccharides	361:375	arg1	lignin					385:390	lignin	385:390	lignin	385:390	This research aimed to investigate the polymerization degree (DP) -dependent effects of chitosan treatments on structural and compositional changes in certain cell wall polysaccharides (mainly lignin), membrane lipids, and proteins of in vitro-propagated Ipomoea purpurea leaves through FT-IR spectroscopy.					
32543784	2	0	theme	naringin	555:562	arg1	diastereoisomers					591:606	eriocitrin, naringin, narirutin, and hesperidin diastereoisomers	543:606	eriocitrin, naringin, narirutin, and hesperidin diastereoisomers	543:606	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
32543784	3	1	theme	rate	767:770	arg1	influence					668:676	The influence	664:676	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition	664:796	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	4	2	theme	acetate	971:977	arg1	pH					986:987	50 mM sodium acetate buffer pH	958:987	50 mM sodium acetate buffer pH	958:987	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	2	3	theme	eriodictyol	417:427	arg1	separation					403:412	The chiral separation	392:412	The chiral separation of eriodictyol, naringenin, and hesperitin	392:455	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
32543784	4	4	theme	mobile	931:936	arg1	phase					938:942	a mobile phase	929:942	a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively	929:1114	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	1	5	theme	C18	314:316	arg1	column					328:333	a C18 capillary column	312:333	a C18 capillary column	312:333	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin.					
32543784	1	6	theme	capillary	318:326	arg1	column					328:333	a C18 capillary column	312:333	a C18 capillary column	312:333	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin.					
32543784	0	7	theme	derivatized	115:125	arg1	β-cyclodextrins					127:141	derivatized β-cyclodextrins	115:141	derivatized β-cyclodextrins	115:141	Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized β-cyclodextrins as mobile-phase additive.					
32543784	3	8	theme	chiral	779:784	arg1	recognition					786:796	the chiral recognition	775:796	the chiral recognition	775:796	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	4	9	theme	optimum	820:826	arg1	conditions					828:837	optimum conditions	820:837	optimum conditions	820:837	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	4	10	theme	carboxymethyl-β-cyclodextrin	1028:1055	arg1	20 mM					1019:1023	20 mM	1019:1023	20 mM of carboxymethyl-β-cyclodextrin	1019:1055	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	4	10	theme	carboxymethyl-β-cyclodextrin	1028:1055	arg1	10 mM					1061:1065	10 mM	1061:1065	10 mM of sulfobutyl ether-β-cyclodextrin	1061:1100	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	6	11	theme	detection	1192:1200	arg1	Limits					1182:1187	Limits	1182:1187	Limits of detection and quantification for the studied flavanone glycosides	1182:1256	Limits of detection and quantification for the studied flavanone glycosides were in the range 1.3-2.5 and 7.5-12.5 µg/mL, respectively.					
32543784	7	12	theme	flavanone-7-O-glycosides	1401:1424	arg1	composition					1382:1392	the diastereomeric composition	1363:1392	the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure	1363:1480	The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure.					
32543784	4	13	theme	baseline	840:847	arg1	separation					849:858	baseline separation	840:858	baseline separation for the selected aglycones and glycosylated forms	840:908	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	1	14	theme	stereoisomer	213:224	arg1	separation					226:235	the stereoisomer separation	209:235	the stereoisomer separation of some flavanone aglycones and 7-O-glycosides	209:282	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin.					
32543784	2	15	theme	eriocitrin	543:552	arg1	diastereoisomers					591:606	eriocitrin, naringin, narirutin, and hesperidin diastereoisomers	543:606	eriocitrin, naringin, narirutin, and hesperidin diastereoisomers	543:606	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
32543784	2	16	theme	hesperitin	446:455	arg1	separation					403:412	The chiral separation	392:412	The chiral separation of eriodictyol, naringenin, and hesperitin	392:455	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
32543784	2	17	theme	mobile	521:526	arg1	phase					528:532	the mobile phase	517:532	the mobile phase	517:532	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
32543784	0	18	theme	nanoliquid	79:88	arg1	chromatography					90:103	nanoliquid chromatography	79:103	nanoliquid chromatography	79:103	Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized β-cyclodextrins as mobile-phase additive.					
32543784	0	19	theme	Stereoisomer	0:11	arg1	separation					13:22	Stereoisomer separation	0:22	Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized β-cyclodextrins as mobile-phase additive.	0:167	Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized β-cyclodextrins as mobile-phase additive.					
32543784	6	20	theme	flavanone	1237:1245	arg1	glycosides					1247:1256	the studied flavanone glycosides	1225:1256	the studied flavanone glycosides	1225:1256	Limits of detection and quantification for the studied flavanone glycosides were in the range 1.3-2.5 and 7.5-12.5 µg/mL, respectively.					
32543784	3	21	from	influence	668:676	arg1	recognition					786:796	the chiral recognition	775:796	the chiral recognition	775:796	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	7	22	theme	Citrus	1429:1434	arg1	juices					1436:1441	Citrus juices	1429:1441	Citrus juices	1429:1441	The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure.					
32543784	0	23	theme	flavanones	27:36	arg1	separation					13:22	Stereoisomer separation	0:22	Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized β-cyclodextrins as mobile-phase additive.	0:167	Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized β-cyclodextrins as mobile-phase additive.					
32543784	7	24	theme	solid-phase	1449:1459	arg1	extraction					1461:1470	solid-phase extraction	1449:1470	solid-phase extraction procedure	1449:1480	The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure.					
32543784	4	25	theme	sulfobutyl	1070:1079	arg1	ether-β-cyclodextrin					1081:1100	sulfobutyl ether-β-cyclodextrin	1070:1100	sulfobutyl ether-β-cyclodextrin	1070:1100	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	6	26	theme	studied	1229:1235	arg1	glycosides					1247:1256	the studied flavanone glycosides	1225:1256	the studied flavanone glycosides	1225:1256	Limits of detection and quantification for the studied flavanone glycosides were in the range 1.3-2.5 and 7.5-12.5 µg/mL, respectively.					
32543784	1	27	theme	flavanone	245:253	arg1	aglycones					255:263	some flavanone aglycones	240:263	some flavanone aglycones	240:263	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin.					
32543784	2	28	theme	carboxymethyl-β-cyclodextrin	485:512	arg1	addition					473:480	addition	473:480	addition of carboxymethyl-β-cyclodextrin to the mobile phase	473:532	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
32543784	1	29	theme	chiral	341:346	arg1	mobile-phase					348:359	a chiral mobile-phase additive	339:368	a chiral mobile-phase additive such as cyclodextrin	339:389	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin.					
32543784	2	30	theme	naringenin	430:439	arg1	separation					403:412	The chiral separation	392:412	The chiral separation of eriodictyol, naringenin, and hesperitin	392:455	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
32543784	3	31	theme	length	722:727	arg1	influence					668:676	The influence	664:676	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition	664:796	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	4	32	gly	glycosylated	891:902	arg1	forms					904:908	glycosylated forms	891:908	glycosylated forms	891:908	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	1	33	theme	aglycones	255:263	arg1	separation					226:235	the stereoisomer separation	209:235	the stereoisomer separation of some flavanone aglycones and 7-O-glycosides	209:282	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin.					
32543784	0	34	theme	flavanone-7-O-glycosides	42:65	arg1	separation					13:22	Stereoisomer separation	0:22	Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized β-cyclodextrins as mobile-phase additive.	0:167	Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized β-cyclodextrins as mobile-phase additive.					
32543784	6	35	theme	quantification	1206:1219	arg1	Limits					1182:1187	Limits	1182:1187	Limits of detection and quantification for the studied flavanone glycosides	1182:1256	Limits of detection and quantification for the studied flavanone glycosides were in the range 1.3-2.5 and 7.5-12.5 µg/mL, respectively.					
32543784	4	36	dep	methanol	999:1006	arg1	%					997:997	%	997:997	%	997:997	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	7	37	theme	composition	1382:1392	arg1	determination					1346:1358	the determination	1342:1358	the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure	1342:1480	The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure.					
32543784	6	38	dep	range	1270:1274	arg1	7.5-12.5 µg/mL					1288:1301	7.5-12.5 µg/mL	1288:1301	7.5-12.5 µg/mL	1288:1301	Limits of detection and quantification for the studied flavanone glycosides were in the range 1.3-2.5 and 7.5-12.5 µg/mL, respectively.					
32543784	6	38	dep	range	1270:1274	arg1	1.3-2.5					1276:1282	1.3-2.5	1276:1282	1.3-2.5	1276:1282	Limits of detection and quantification for the studied flavanone glycosides were in the range 1.3-2.5 and 7.5-12.5 µg/mL, respectively.					
32543784	1	39	theme	additive	361:368	arg1	mobile-phase					348:359	a chiral mobile-phase additive	339:368	a chiral mobile-phase additive such as cyclodextrin	339:389	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin.					
32543784	4	40	dep	%	997:997	arg1	3					989:989	3	989:989	3	989:989	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	4	40	dep	%	997:997	arg1	30					995:996	30	995:996	30	995:996	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	5	41	theme	method	1162:1167	arg1	linearity					1128:1136	linearity	1128:1136	linearity	1128:1136	Precision, linearity, and sensitivity of the method were tested.					
32543784	5	41	theme	method	1162:1167	arg1	sensitivity					1143:1153	sensitivity	1143:1153	sensitivity	1143:1153	Precision, linearity, and sensitivity of the method were tested.					
32543784	5	41	theme	method	1162:1167	arg1	Precision					1117:1125	Precision	1117:1125	Precision	1117:1125	Precision, linearity, and sensitivity of the method were tested.					
32543784	3	42	theme	composition	685:695	arg1	influence					668:676	The influence	664:676	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition	664:796	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	4	43	contain	containing	1008:1017	arg2	10 mM					1061:1065	10 mM	1061:1065	10 mM of sulfobutyl ether-β-cyclodextrin	1061:1100	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	4	43	contain	containing	1008:1017	arg2	20 mM					1019:1023	20 mM	1019:1023	20 mM of carboxymethyl-β-cyclodextrin	1019:1055	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	4	43	contain	containing	1008:1017	arg1	methanol					999:1006	3 and 30% methanol	989:1006	3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin	989:1100	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	1	44	theme	7-O-glycosides	269:282	arg1	separation					226:235	the stereoisomer separation	209:235	the stereoisomer separation of some flavanone aglycones and 7-O-glycosides	209:282	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin.					
32543784	4	45	theme	sodium	964:969	arg1	pH					986:987	50 mM sodium acetate buffer pH	958:987	50 mM sodium acetate buffer pH	958:987	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	7	46	from	composition	1382:1392	arg1	juices					1436:1441	Citrus juices	1429:1441	Citrus juices	1429:1441	The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure.					
32543784	4	47	theme	ether-β-cyclodextrin	1081:1100	arg1	20 mM					1019:1023	20 mM	1019:1023	20 mM of carboxymethyl-β-cyclodextrin	1019:1055	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	4	47	theme	ether-β-cyclodextrin	1081:1100	arg1	10 mM					1061:1065	10 mM	1061:1065	10 mM of sulfobutyl ether-β-cyclodextrin	1061:1100	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	4	48	theme	50 mM	958:962	arg1	pH					986:987	50 mM sodium acetate buffer pH	958:987	50 mM sodium acetate buffer pH	958:987	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	3	49	theme	column	746:751	arg1	length					722:727	the length	718:727	the length of the capillary column	718:751	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	3	49	theme	column	746:751	arg1	composition					685:695	the composition	681:695	the composition of the mobile phase	681:715	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	3	49	theme	column	746:751	arg1	rate					767:770	the flow rate	758:770	the flow rate	758:770	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	4	50	theme	glycosylated	891:902	arg1	forms					904:908	glycosylated forms	891:908	glycosylated forms	891:908	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	2	51	theme	chiral	396:401	arg1	separation					403:412	The chiral separation	392:412	The chiral separation of eriodictyol, naringenin, and hesperitin	392:455	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
32543784	4	52	theme	buffer	979:984	arg1	pH					986:987	50 mM sodium acetate buffer pH	958:987	50 mM sodium acetate buffer pH	958:987	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	7	53	used	used	1333:1336	arg2	method					1322:1327	The method	1318:1327	The method	1318:1327	The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure.					
32543784	3	54	theme	mobile	704:709	arg1	phase					711:715	the mobile phase	700:715	the mobile phase	700:715	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	7	55	from	flavanone-7-O-glycosides	1401:1424	arg1	juices					1436:1441	Citrus juices	1429:1441	Citrus juices	1429:1441	The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure.					
32543784	2	56	theme	sulfobutyl	631:640	arg1	ether-β-cyclodextrin					642:661	sulfobutyl ether-β-cyclodextrin	631:661	sulfobutyl ether-β-cyclodextrin	631:661	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
32543784	7	57	theme	diastereomeric	1367:1380	arg1	composition					1382:1392	the diastereomeric composition	1363:1392	the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure	1363:1480	The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure.					
32543784	3	58	theme	phase	711:715	arg1	length					722:727	the length	718:727	the length of the capillary column	718:751	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	3	58	theme	phase	711:715	arg1	composition					685:695	the composition	681:695	the composition of the mobile phase	681:715	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	3	58	theme	phase	711:715	arg1	rate					767:770	the flow rate	758:770	the flow rate	758:770	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	1	59	theme	nanoliquid	171:180	arg1	method					198:203	A nanoliquid chromatographic method	169:203	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides	169:282	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin.					
32543784	2	60	theme	narirutin	565:573	arg1	diastereoisomers					591:606	eriocitrin, naringin, narirutin, and hesperidin diastereoisomers	543:606	eriocitrin, naringin, narirutin, and hesperidin diastereoisomers	543:606	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
32543784	3	61	theme	capillary	736:744	arg1	column					746:751	the capillary column	732:751	the capillary column	732:751	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	4	62	theme	selected	868:875	arg1	aglycones					877:885	the selected aglycones	864:885	the selected aglycones	864:885	At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-β-cyclodextrin and 10 mM of sulfobutyl ether-β-cyclodextrin, respectively.					
32543784	1	63	theme	chromatographic	182:196	arg1	method					198:203	A nanoliquid chromatographic method	169:203	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides	169:282	A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin.					
32543784	3	64	theme	flow	762:765	arg1	rate					767:770	the flow rate	758:770	the flow rate	758:770	The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated.					
32543784	7	65	theme	extraction	1461:1470	arg1	procedure					1472:1480	solid-phase extraction procedure	1449:1480	solid-phase extraction procedure	1449:1480	The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure.					
32543784	7	66	from	juices	1436:1441	arg1	composition					1382:1392	the diastereomeric composition	1363:1392	the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure	1363:1480	The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides in Citrus juices after solid-phase extraction procedure.					
32543784	2	67	theme	hesperidin	580:589	arg1	diastereoisomers					591:606	eriocitrin, naringin, narirutin, and hesperidin diastereoisomers	543:606	eriocitrin, naringin, narirutin, and hesperidin diastereoisomers	543:606	The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-β-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-β-cyclodextrin.					
33525574	0	0	theme	High-Mannose	66:77	arg1	Glycans					79:85	High-Mannose Glycans	66:85	High-Mannose Glycans on Microorganisms	66:103	BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms.					
33525574	7	1	from	sites	1465:1469	arg1	microorganisms					1484:1497	different microorganisms	1474:1497	different microorganisms	1474:1497	BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms.					
33525574	2	2	theme	molecular	620:628	arg1	mechanics					630:638	molecular mechanics	620:638	molecular mechanics	620:638	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	3	theme	glycans	322:328	arg1	evaluation					295:304	the evaluation	291:304	the evaluation of high-mannose glycans present on various glycoproteins	291:361	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	4	dep	protein	560:566	arg1	eGFP					569:572	eGFP	569:572	eGFP	569:572	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	4	dep	protein	560:566	arg1	4EUL					579:582	PDB 4EUL	575:582	PDB 4EUL	575:582	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	7	5	theme	useful	1412:1417	arg1	tool					1419:1422	a useful tool	1410:1422	a useful tool for screening high-mannose glycosylation sites on different microorganisms	1410:1497	BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms.					
33525574	7	5	theme	useful	1412:1417	arg1	BanLec-eGFP					1391:1401	BanLec-eGFP	1391:1401	BanLec-eGFP	1391:1401	BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms.					
33525574	2	6	theme	crystal	432:438	arg1	structures					440:449	the crystal structures	428:449	the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL)	428:583	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	5	7	with	assay	1110:1114	arg1	type					1176:1179	wild type	1171:1179	wild type	1171:1179	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	7	with	assay	1110:1114	arg1	mannose					1146:1152	mannose	1146:1152	mannose	1146:1152	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	7	with	assay	1110:1114	arg1	glucose					1158:1164	glucose	1158:1164	glucose	1158:1164	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	7	with	assay	1110:1114	arg1	monosaccharides					1129:1143	monosaccharides	1129:1143	monosaccharides (mannose and glucose)	1129:1165	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	1	8	theme	in	157:158	arg1	studies					178:184	in vivo and in vitro studies	157:184	in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi	157:284	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	4	9	theme	primary	913:919	arg1	structure					921:929	the primary structure	909:929	the primary structure	909:929	More than 65% of the primary structure was confirmed by mass spectrometry.					
33525574	1	10	theme	various	231:237	arg1	fungi					280:284	fungi	280:284	fungi	280:284	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	1	10	theme	various	231:237	arg1	bacteria					266:273	bacteria	266:273	bacteria	266:273	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	1	10	theme	various	231:237	arg1	pathogens					239:247	various pathogens	231:247	various pathogens	231:247	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	1	10	theme	various	231:237	arg1	viruses					257:263	viruses	257:263	viruses	257:263	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	4	11	theme	mass	948:951	arg1	spectrometry					953:964	mass spectrometry	948:964	mass spectrometry	948:964	More than 65% of the primary structure was confirmed by mass spectrometry.					
33525574	5	12	theme	high	1002:1005	arg1	glycans					1015:1021	high mannose glycans	1002:1021	high mannose glycans of the influenza vaccine (Vaxigrip®)	1002:1058	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	6	13	theme	flow	1300:1303	arg1	cytometry					1305:1313	flow cytometry	1300:1313	flow cytometry	1300:1313	BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate.					
33525574	1	14	theme	pathogens	239:247	arg1	function					207:214	function	207:214	function	207:214	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	1	14	theme	pathogens	239:247	arg1	structure					193:201	structure	193:201	structure	193:201	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	5	15	theme	sorbent	1102:1108	arg1	FLLSA					1117:1121	FLLSA	1117:1121	FLLSA	1117:1121	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	15	theme	sorbent	1102:1108	arg1	assay					1110:1114	a fluorescence-linked lectin sorbent assay	1073:1114	a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant	1073:1209	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	3	16	theme	wavelength	872:881	arg1	nm					887:888	excitation wavelength 488 nm	861:888	excitation wavelength 488 nm	861:888	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	3	16	theme	wavelength	872:881	arg1	nm					857:858	509 nm	853:858	509 nm (excitation wavelength 488 nm)	853:889	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	3	17	theme	secondary	788:796	arg1	β-sheets					754:761	β-sheets	754:761	β-sheets (41%)	754:767	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	3	17	theme	secondary	788:796	arg1	structures					798:807	the predominant secondary structures	772:807	the predominant secondary structures	772:807	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	2	18	theme	lectin	473:478	arg1	structures					440:449	the crystal structures	428:449	the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL)	428:583	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	5	19	theme	wild	1171:1174	arg1	type					1176:1179	wild type	1171:1179	wild type	1171:1179	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	2	20	theme	fluorescent	548:558	arg1	protein					560:566	an enhanced green fluorescent protein	530:566	an enhanced green fluorescent protein (eGFP, PDB 4EUL)	530:583	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	21	theme	protein	560:566	arg1	structures					440:449	the crystal structures	428:449	the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL)	428:583	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	22	theme	banana	466:471	arg1	lectin					473:478	recombinant banana lectin	454:478	recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG)	454:524	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	22	theme	banana	466:471	arg1	BanLec					481:486	BanLec	481:486	BanLec	481:486	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	23	theme	green	542:546	arg1	protein					560:566	an enhanced green fluorescent protein	530:566	an enhanced green fluorescent protein (eGFP, PDB 4EUL)	530:583	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	7	24	gly	glycosylation	1451:1463	arg2	sites					1465:1469	high-mannose glycosylation sites	1438:1469	high-mannose glycosylation sites on different microorganisms	1438:1497	BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms.					
33525574	5	25	theme	fluorescence-linked	1075:1093	arg1	FLLSA					1117:1121	FLLSA	1117:1121	FLLSA	1117:1121	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	25	theme	fluorescence-linked	1075:1093	arg1	assay					1110:1114	a fluorescence-linked lectin sorbent assay	1073:1114	a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant	1073:1209	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	26	theme	BanLec	1181:1186	arg1	mutant					1204:1209	BanLec and H84T BanLec mutant	1181:1209	BanLec and H84T BanLec mutant	1181:1209	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	2	27	theme	chimera	402:408	arg1	model					389:393	a three-dimensional (3D) model	364:393	a three-dimensional (3D) model of the chimera	364:408	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	5	28	theme	Competitive	967:977	arg1	binding					991:997	Competitive BanLec-eGFP binding	967:997	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®)	967:1058	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	2	29	theme	enhanced	533:540	arg1	protein					560:566	an enhanced green fluorescent protein	530:566	an enhanced green fluorescent protein (eGFP, PDB 4EUL)	530:583	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	30	theme	recombinant	454:464	arg1	lectin					473:478	recombinant banana lectin	454:478	recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG)	454:524	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	30	theme	recombinant	454:464	arg1	BanLec					481:486	BanLec	481:486	BanLec	481:486	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	3	31	theme	predominant	776:786	arg1	β-sheets					754:761	β-sheets	754:761	β-sheets (41%)	754:767	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	3	31	theme	predominant	776:786	arg1	structures					798:807	the predominant secondary structures	772:807	the predominant secondary structures	772:807	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	1	32	theme	in	169:170	arg1	studies					178:184	in vivo and in vitro studies	157:184	in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi	157:284	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	2	33	dep	BanLec	481:486	arg1	PDB					514:516	PDB	514:516	PDB	514:516	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	33	dep	BanLec	481:486	arg1	5EXG					520:523	5EXG	520:523	5EXG	520:523	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	33	dep	BanLec	481:486	arg1	entry					507:511	Protein Data Bank entry	489:511	Protein Data Bank entry (PDB)	489:517	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	5	34	theme	influenza	1030:1038	arg1	vaccine					1040:1046	the influenza vaccine	1026:1046	the influenza vaccine (Vaxigrip®)	1026:1058	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	34	theme	influenza	1030:1038	arg1	Vaxigrip®					1049:1057	Vaxigrip®	1049:1057	Vaxigrip®	1049:1057	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	2	35	gly	glycoproteins	349:361	arg1	glycoproteins					349:361	various glycoproteins	341:361	various glycoproteins	341:361	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	1	36	dep	in	157:158	arg1	vivo					160:163	vivo	160:163	vivo	160:163	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	6	37	theme	clinical	1373:1380	arg1	isolate					1382:1388	a Salmonella Typhi clinical isolate	1354:1388	a Salmonella Typhi clinical isolate	1354:1388	BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate.					
33525574	2	38	theme	various	341:347	arg1	glycoproteins					349:361	various glycoproteins	341:361	various glycoproteins	341:361	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	5	39	theme	vaccine	1040:1046	arg1	glycans					1015:1021	high mannose glycans	1002:1021	high mannose glycans of the influenza vaccine (Vaxigrip®)	1002:1058	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	2	40	theme	Bank	502:505	arg1	PDB					514:516	PDB	514:516	PDB	514:516	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	40	theme	Bank	502:505	arg1	entry					507:511	Protein Data Bank entry	489:511	Protein Data Bank entry (PDB)	489:517	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	41	theme	PDB	575:577	arg1	eGFP					569:572	eGFP	569:572	eGFP	569:572	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	41	theme	PDB	575:577	arg1	4EUL					579:582	PDB 4EUL	575:582	PDB 4EUL	575:582	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	3	42	theme	emission	819:826	arg1	maximum					833:839	the emission peak maximum	815:839	the emission peak maximum detected at 509 nm (excitation wavelength 488 nm)	815:889	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	2	43	theme	three-dimensional	366:382	arg1	model					389:393	a three-dimensional (3D) model	364:393	a three-dimensional (3D) model of the chimera	364:408	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	5	44	theme	BanLec	1197:1202	arg1	mutant					1204:1209	BanLec and H84T BanLec mutant	1181:1209	BanLec and H84T BanLec mutant	1181:1209	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	2	45	theme	Data	497:500	arg1	PDB					514:516	PDB	514:516	PDB	514:516	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	45	theme	Data	497:500	arg1	entry					507:511	Protein Data Bank entry	489:511	Protein Data Bank entry (PDB)	489:517	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	46	theme	high-mannose	309:320	arg1	glycans					322:328	high-mannose glycans	309:328	high-mannose glycans present on various glycoproteins	309:361	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	6	47	theme	Salmonella	1356:1365	arg1	Typhi					1367:1371	Salmonella Typhi	1356:1371	a Salmonella Typhi clinical isolate	1354:1388	BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate.					
33525574	7	48	theme	high-mannose	1438:1449	arg1	sites					1465:1469	high-mannose glycosylation sites	1438:1469	high-mannose glycosylation sites on different microorganisms	1438:1497	BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms.					
33525574	6	49	theme	different	1265:1273	arg1	strains					1275:1281	different strains	1265:1281	different strains of Salmonella	1265:1295	BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate.					
33525574	2	50	theme	Protein	489:495	arg1	PDB					514:516	PDB	514:516	PDB	514:516	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	50	theme	Protein	489:495	arg1	entry					507:511	Protein Data Bank entry	489:511	Protein Data Bank entry (PDB)	489:517	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	1	51	dep	in	169:170	arg1	vitro					172:176	vitro	172:176	vitro	172:176	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	5	52	theme	mannose	1007:1013	arg1	glycans					1015:1021	high mannose glycans	1002:1021	high mannose glycans of the influenza vaccine (Vaxigrip®)	1002:1058	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	3	53	theme	kDa	740:742	arg1	protein					720:726	a soluble cytosolic protein	700:726	a soluble cytosolic protein of about 42 kDa	700:742	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	2	54	dep	three-dimensional	366:382	arg1	3D					385:386	3D	385:386	3D	385:386	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	1	55	theme	structure	193:201	arg1	studies					178:184	in vivo and in vitro studies	157:184	in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi	157:284	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	7	56	theme	different	1474:1482	arg1	microorganisms					1484:1497	different microorganisms	1474:1497	different microorganisms	1474:1497	BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms.					
33525574	7	57	theme	glycosylation	1451:1463	arg1	sites					1465:1469	high-mannose glycosylation sites	1438:1469	high-mannose glycosylation sites on different microorganisms	1438:1497	BanLec-eGFP can be a useful tool for screening high-mannose glycosylation sites on different microorganisms.					
33525574	6	58	theme	Salmonella	1286:1295	arg1	strains					1275:1281	different strains	1265:1281	different strains of Salmonella	1265:1295	BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate.					
33525574	3	59	theme	peak	828:831	arg1	maximum					833:839	the emission peak maximum	815:839	the emission peak maximum detected at 509 nm (excitation wavelength 488 nm)	815:889	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	5	60	theme	BanLec-eGFP	979:989	arg1	binding					991:997	Competitive BanLec-eGFP binding	967:997	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®)	967:1058	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	2	61	theme	molecular	597:605	arg1	modeling					607:614	molecular modeling	597:614	molecular modeling	597:614	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	1	62	theme	labeled	120:126	arg1	lectins					128:134	Fluorescently labeled lectins	106:134	Fluorescently labeled lectins	106:134	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	1	62	theme	labeled	120:126	arg1	tools					147:151	useful tools	140:151	useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi	140:284	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	5	63	link	fluorescence-linked	1075:1093	arg1	FLLSA					1117:1121	FLLSA	1117:1121	FLLSA	1117:1121	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	63	link	fluorescence-linked	1075:1093	arg1	assay					1110:1114	a fluorescence-linked lectin sorbent assay	1073:1114	a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant	1073:1209	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	6	64	theme	Typhi	1367:1371	arg1	isolate					1382:1388	a Salmonella Typhi clinical isolate	1354:1388	a Salmonella Typhi clinical isolate	1354:1388	BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate.					
33525574	5	65	dep	monosaccharides	1129:1143	arg1	mutant					1204:1209	BanLec and H84T BanLec mutant	1181:1209	BanLec and H84T BanLec mutant	1181:1209	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	65	dep	monosaccharides	1129:1143	arg1	monosaccharides					1129:1143	monosaccharides	1129:1143	monosaccharides (mannose and glucose)	1129:1165	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	65	dep	monosaccharides	1129:1143	arg1	glucose					1158:1164	glucose	1158:1164	glucose	1158:1164	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	65	dep	monosaccharides	1129:1143	arg1	mannose					1146:1152	mannose	1146:1152	mannose	1146:1152	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	4	66	theme	structure	921:929	arg1	%					904:904	More than 65%	892:904	More than 65% of the primary structure	892:929	More than 65% of the primary structure was confirmed by mass spectrometry.					
33525574	4	66	theme	structure	921:929	arg1	structure					921:929	the primary structure	909:929	the primary structure	909:929	More than 65% of the primary structure was confirmed by mass spectrometry.					
33525574	3	67	theme	cytosolic	710:718	arg1	protein					720:726	a soluble cytosolic protein	700:726	a soluble cytosolic protein of about 42 kDa	700:742	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	1	68	dep	structure	193:201	arg1	the					189:191	the	189:191	the	189:191	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	3	69	located	detected	841:848	arg1	nm					887:888	excitation wavelength 488 nm	861:888	excitation wavelength 488 nm	861:888	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	3	69	located	detected	841:848	arg1	nm					857:858	509 nm	853:858	509 nm (excitation wavelength 488 nm)	853:889	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	3	69	located	detected	841:848	arg2	maximum					833:839	the emission peak maximum	815:839	the emission peak maximum detected at 509 nm (excitation wavelength 488 nm)	815:889	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	6	70	theme	pronounced	1332:1341	arg1	binding					1343:1349	especially pronounced binding	1321:1349	especially pronounced binding to a Salmonella Typhi clinical isolate	1321:1388	BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate.					
33525574	1	71	theme	function	207:214	arg1	studies					178:184	in vivo and in vitro studies	157:184	in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi	157:284	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	0	72	theme	Binding	55:61	arg1	Evaluation					34:43	Evaluation	34:43	Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms	34:103	BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms.					
33525574	0	73	from	Glycans	79:85	arg1	Microorganisms					90:103	Microorganisms	90:103	Microorganisms	90:103	BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms.					
33525574	3	74	theme	excitation	861:870	arg1	nm					887:888	excitation wavelength 488 nm	861:888	excitation wavelength 488 nm	861:888	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	3	74	theme	excitation	861:870	arg1	nm					857:858	509 nm	853:858	509 nm (excitation wavelength 488 nm)	853:889	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	1	75	theme	useful	140:145	arg1	lectins					128:134	Fluorescently labeled lectins	106:134	Fluorescently labeled lectins	106:134	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	1	75	theme	useful	140:145	arg1	tools					147:151	useful tools	140:151	useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi	140:284	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	5	76	theme	lectin	1095:1100	arg1	FLLSA					1117:1121	FLLSA	1117:1121	FLLSA	1117:1121	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	5	76	theme	lectin	1095:1100	arg1	assay					1110:1114	a fluorescence-linked lectin sorbent assay	1073:1114	a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant	1073:1209	Competitive BanLec-eGFP binding to high mannose glycans of the influenza vaccine (Vaxigrip®) was shown in a fluorescence-linked lectin sorbent assay (FLLSA) with monosaccharides (mannose and glucose) and wild type BanLec and H84T BanLec mutant.					
33525574	0	77	theme	Lectin	48:53	arg1	Binding					55:61	Lectin Binding	48:61	Lectin Binding to High-Mannose Glycans on Microorganisms	48:103	BanLec-eGFP Chimera as a Tool for Evaluation of Lectin Binding to High-Mannose Glycans on Microorganisms.					
33525574	2	78	attach	present	330:336	arg2	glycans					322:328	high-mannose glycans	309:328	high-mannose glycans present on various glycoproteins	309:361	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	2	78	attach	present	330:336	arg1	glycoproteins					349:361	various glycoproteins	341:361	various glycoproteins	341:361	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	3	79	theme	soluble	702:708	arg1	protein					720:726	a soluble cytosolic protein	700:726	a soluble cytosolic protein of about 42 kDa	700:742	BanLec-eGFP, produced as a soluble cytosolic protein of about 42 kDa, revealed β-sheets (41%) as the predominant secondary structures, with the emission peak maximum detected at 509 nm (excitation wavelength 488 nm).					
33525574	6	80	theme	mannose	1245:1251	arg1	residues					1253:1260	mannose residues	1245:1260	mannose residues	1245:1260	BanLec-eGFP exhibited binding to mannose residues on different strains of Salmonella in flow cytometry, with especially pronounced binding to a Salmonella Typhi clinical isolate.					
33525574	2	81	theme	present	330:336	arg1	glycans					322:328	high-mannose glycans	309:328	high-mannose glycans present on various glycoproteins	309:361	For the evaluation of high-mannose glycans present on various glycoproteins, a three-dimensional (3D) model of the chimera was designed from the crystal structures of recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) and an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling and molecular mechanics and expressed in Escherichia coli.					
33525574	1	82	theme	tissues	219:225	arg1	function					207:214	function	207:214	function	207:214	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
33525574	1	82	theme	tissues	219:225	arg1	structure					193:201	structure	193:201	structure	193:201	Fluorescently labeled lectins are useful tools for in vivo and in vitro studies of the structure and function of tissues and various pathogens such as viruses, bacteria, and fungi.					
32788422	6	0	theme	strongest	900:908	arg1	association					910:920	the strongest association	896:920	the strongest association	896:920	Country of residence associated with many N-glycan features and the strongest association was with monogalactosylation where it explained 38% of variability.					
32788422	7	1	theme	development	1126:1136	arg1	indicators					1138:1147	United Nations health and socioeconomic development indicators	1086:1147	indicators	1138:1147	IgG monogalactosylation strongly correlated with the development level of a country, defined by United Nations health and socioeconomic development indicators, and with the expected lifespan.					
32788422	9	2	theme	low-grade	1413:1421	arg1	inflammation					1431:1442	low-grade chronic inflammation	1413:1442	low-grade chronic inflammation	1413:1442	Our results suggest that citizens of developing countries may be exposed to environmental factors that can cause low-grade chronic inflammation and the apparent increase in biological age.					
32788422	9	3	theme	apparent	1452:1459	arg1	increase					1461:1468	the apparent increase	1448:1468	the apparent increase in biological age	1448:1486	Our results suggest that citizens of developing countries may be exposed to environmental factors that can cause low-grade chronic inflammation and the apparent increase in biological age.					
32788422	8	4	from	countries	1207:1215	arg1	Subjects					1182:1189	Subjects	1182:1189	Subjects from developing countries	1182:1215	Subjects from developing countries had low levels of IgG galactosylation, characteristic for inflammation and ageing.					
32788422	4	5	theme	inter-population	489:504	arg1	differences					506:516	global inter-population differences	482:516	global inter-population differences in IgG glycosylation	482:537	However, global inter-population differences in IgG glycosylation have never been properly addressed.					
32788422	1	6	theme	effector	141:148	arg1	functions					150:158	effector functions	141:158	effector functions	141:158	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	4	7	theme	global	482:487	arg1	differences					506:516	global inter-population differences	482:516	global inter-population differences in IgG glycosylation	482:537	However, global inter-population differences in IgG glycosylation have never been properly addressed.					
32788422	5	8	theme	different	658:666	arg1	populations					668:678	5 different populations	656:678	5 different populations totaling 10,482 IgG glycomes	656:707	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	5	9	theme	population-specific	591:609	arg1	patterns					627:634	population-specific N-glycosylation patterns	591:634	population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world	591:829	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	1	10	theme	Immunoglobulin	45:58	arg1	antibody					95:102	the most abundant serum antibody	71:102	the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans	71:236	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	1	10	theme	Immunoglobulin	45:58	arg1	IgG					63:65	IgG	63:65	IgG	63:65	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	1	10	theme	Immunoglobulin	45:58	arg1	G					60:60	Immunoglobulin G	45:60	Immunoglobulin G (IgG)	45:66	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	6	11	theme	variability	977:987	arg1	variability					977:987	variability	977:987	variability	977:987	Country of residence associated with many N-glycan features and the strongest association was with monogalactosylation where it explained 38% of variability.					
32788422	6	11	theme	variability	977:987	arg1	%					972:972	38%	970:972	38% of variability	970:987	Country of residence associated with many N-glycan features and the strongest association was with monogalactosylation where it explained 38% of variability.					
32788422	3	12	theme	IgG	348:350	arg1	glycosylation					352:364	IgG glycosylation	348:364	IgG glycosylation	348:364	Variability of IgG glycosylation within a population is well studied and is known to be affected by both genetic and environmental factors.					
32788422	5	13	from	populations	794:804	arg1	samples					778:784	2,579 samples	772:784	2,579 samples from 27 populations sampled across the world	772:829	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	7	14	theme	socioeconomic	1112:1124	arg1	indicators					1138:1147	United Nations health and socioeconomic development indicators	1086:1147	indicators	1138:1147	IgG monogalactosylation strongly correlated with the development level of a country, defined by United Nations health and socioeconomic development indicators, and with the expected lifespan.					
32788422	5	15	theme	N-glycosylation	611:625	arg1	patterns					627:634	population-specific N-glycosylation patterns	591:634	population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world	591:829	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	5	16	theme	crystallizable	732:745	arg1	Fc					755:756	Fc	755:756	Fc	755:756	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	5	16	theme	crystallizable	732:745	arg1	region					747:752	IgG's fragment crystallizable region	717:752	IgG's fragment crystallizable region (Fc)	717:757	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	6	17	theme	N-glycan	874:881	arg1	features					883:890	many N-glycan features	869:890	many N-glycan features	869:890	Country of residence associated with many N-glycan features and the strongest association was with monogalactosylation where it explained 38% of variability.					
32788422	7	18	theme	Nations	1093:1099	arg1	health					1101:1106	United Nations health and socioeconomic development indicators	1086:1147	health	1101:1106	IgG monogalactosylation strongly correlated with the development level of a country, defined by United Nations health and socioeconomic development indicators, and with the expected lifespan.					
32788422	2	19	theme	N-glycome	262:270	arg1	Composition					239:249	Composition	239:249	Composition of the IgG N-glycome	239:270	Composition of the IgG N-glycome changes with age of an individual and in different diseases.					
32788422	4	20	from	differences	506:516	arg1	glycosylation					525:537	IgG glycosylation	521:537	IgG glycosylation	521:537	However, global inter-population differences in IgG glycosylation have never been properly addressed.					
32788422	6	21	theme	many	869:872	arg1	features					883:890	many N-glycan features	869:890	many N-glycan features	869:890	Country of residence associated with many N-glycan features and the strongest association was with monogalactosylation where it explained 38% of variability.					
32788422	5	22	theme	IgG	639:641	arg1	patterns					627:634	population-specific N-glycosylation patterns	591:634	population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world	591:829	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	2	23	theme	IgG	258:260	arg1	N-glycome					262:270	the IgG N-glycome	254:270	the IgG N-glycome	254:270	Composition of the IgG N-glycome changes with age of an individual and in different diseases.					
32788422	8	24	theme	galactosylation	1239:1253	arg1	levels					1225:1230	low levels	1221:1230	low levels	1221:1230	Subjects from developing countries had low levels of IgG galactosylation, characteristic for inflammation and ageing.					
32788422	8	25	theme	developing	1196:1205	arg1	countries					1207:1215	developing countries	1196:1215	developing countries	1196:1215	Subjects from developing countries had low levels of IgG galactosylation, characteristic for inflammation and ageing.					
32788422	9	26	theme	countries	1348:1356	arg1	citizens					1325:1332	citizens	1325:1332	citizens of developing countries	1325:1356	Our results suggest that citizens of developing countries may be exposed to environmental factors that can cause low-grade chronic inflammation and the apparent increase in biological age.					
32788422	2	27	theme	individual	295:304	arg1	age					285:287	age	285:287	age of an individual	285:304	Composition of the IgG N-glycome changes with age of an individual and in different diseases.					
32788422	9	28	from	inflammation	1431:1442	arg1	age					1484:1486	biological age	1473:1486	biological age	1473:1486	Our results suggest that citizens of developing countries may be exposed to environmental factors that can cause low-grade chronic inflammation and the apparent increase in biological age.					
32788422	0	29	theme	Global	0:5	arg1	variability					7:17	Global variability	0:17	Global variability of the human IgG glycome	0:42	Global variability of the human IgG glycome.					
32788422	7	30	theme	United	1086:1091	arg1	health					1101:1106	United Nations health and socioeconomic development indicators	1086:1147	health	1101:1106	IgG monogalactosylation strongly correlated with the development level of a country, defined by United Nations health and socioeconomic development indicators, and with the expected lifespan.					
32788422	8	31	theme	characteristic	1256:1269	arg1	levels					1225:1230	low levels	1221:1230	low levels	1221:1230	Subjects from developing countries had low levels of IgG galactosylation, characteristic for inflammation and ageing.					
32788422	3	32	theme	glycosylation	352:364	arg1	Variability					333:343	Variability	333:343	Variability of IgG glycosylation within a population	333:384	Variability of IgG glycosylation within a population is well studied and is known to be affected by both genetic and environmental factors.					
32788422	9	33	from	increase	1461:1468	arg1	age					1484:1486	biological age	1473:1486	biological age	1473:1486	Our results suggest that citizens of developing countries may be exposed to environmental factors that can cause low-grade chronic inflammation and the apparent increase in biological age.					
32788422	3	34	theme	genetic	438:444	arg1	factors					464:470	both genetic and environmental factors	433:470	both genetic and environmental factors	433:470	Variability of IgG glycosylation within a population is well studied and is known to be affected by both genetic and environmental factors.					
32788422	0	35	theme	IgG	32:34	arg1	glycome					36:42	the human IgG glycome	22:42	the human IgG glycome	22:42	Global variability of the human IgG glycome.					
32788422	2	36	theme	different	313:321	arg1	diseases					323:330	different diseases	313:330	different diseases	313:330	Composition of the IgG N-glycome changes with age of an individual and in different diseases.					
32788422	6	37	theme	residence	843:851	arg1	Country					832:838	Country	832:838	Country of residence	832:851	Country of residence associated with many N-glycan features and the strongest association was with monogalactosylation where it explained 38% of variability.					
32788422	0	38	theme	human	26:30	arg1	glycome					36:42	the human IgG glycome	22:42	the human IgG glycome	22:42	Global variability of the human IgG glycome.					
32788422	9	39	theme	environmental	1376:1388	arg1	factors					1390:1396	environmental factors	1376:1396	environmental factors that can cause low-grade chronic inflammation and the apparent increase in biological age	1376:1486	Our results suggest that citizens of developing countries may be exposed to environmental factors that can cause low-grade chronic inflammation and the apparent increase in biological age.					
32788422	7	40	theme	country	1066:1072	arg1	level					1055:1059	the development level	1039:1059	the development level of a country, defined by United Nations health and socioeconomic development indicators	1039:1147	IgG monogalactosylation strongly correlated with the development level of a country, defined by United Nations health and socioeconomic development indicators, and with the expected lifespan.					
32788422	5	41	theme	fragment	723:730	arg1	Fc					755:756	Fc	755:756	Fc	755:756	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	5	41	theme	fragment	723:730	arg1	region					747:752	IgG's fragment crystallizable region	717:752	IgG's fragment crystallizable region (Fc)	717:757	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	7	42	theme	expected	1163:1170	arg1	lifespan					1172:1179	the expected lifespan	1159:1179	the expected lifespan	1159:1179	IgG monogalactosylation strongly correlated with the development level of a country, defined by United Nations health and socioeconomic development indicators, and with the expected lifespan.					
32788422	1	43	theme	various	200:206	arg1	moieties					214:221	various sugar moieties	200:221	various sugar moieties called glycans	200:236	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	5	44	theme	2,579	772:776	arg1	samples					778:784	2,579 samples	772:784	2,579 samples from 27 populations sampled across the world	772:829	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	8	45	theme	low	1221:1223	arg1	levels					1225:1230	low levels	1221:1230	low levels	1221:1230	Subjects from developing countries had low levels of IgG galactosylation, characteristic for inflammation and ageing.					
32788422	9	46	theme	developing	1337:1346	arg1	countries					1348:1356	developing countries	1337:1356	developing countries	1337:1356	Our results suggest that citizens of developing countries may be exposed to environmental factors that can cause low-grade chronic inflammation and the apparent increase in biological age.					
32788422	1	47	theme	abundant	80:87	arg1	antibody					95:102	the most abundant serum antibody	71:102	the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans	71:236	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	1	47	theme	abundant	80:87	arg1	G					60:60	Immunoglobulin G	45:60	Immunoglobulin G (IgG)	45:66	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	0	48	theme	glycome	36:42	arg1	variability					7:17	Global variability	0:17	Global variability of the human IgG glycome	0:42	Global variability of the human IgG glycome.					
32788422	5	49	theme	10,482	689:694	arg1	glycomes					700:707	10,482 IgG glycomes	689:707	10,482 IgG glycomes	689:707	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	1	50	theme	serum	89:93	arg1	antibody					95:102	the most abundant serum antibody	71:102	the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans	71:236	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	1	50	theme	serum	89:93	arg1	G					60:60	Immunoglobulin G	45:60	Immunoglobulin G (IgG)	45:66	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	4	51	theme	IgG	521:523	arg1	glycosylation					525:537	IgG glycosylation	521:537	IgG glycosylation	521:537	However, global inter-population differences in IgG glycosylation have never been properly addressed.					
32788422	5	52	theme	IgG	696:698	arg1	glycomes					700:707	10,482 IgG glycomes	689:707	10,482 IgG glycomes	689:707	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32788422	1	53	theme	sugar	208:212	arg1	moieties					214:221	various sugar moieties	200:221	various sugar moieties called glycans	200:236	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	7	54	theme	development	1043:1053	arg1	level					1055:1059	the development level	1039:1059	the development level of a country, defined by United Nations health and socioeconomic development indicators	1039:1147	IgG monogalactosylation strongly correlated with the development level of a country, defined by United Nations health and socioeconomic development indicators, and with the expected lifespan.					
32788422	8	55	contain	had	1217:1219	arg2	levels					1225:1230	low levels	1221:1230	low levels	1221:1230	Subjects from developing countries had low levels of IgG galactosylation, characteristic for inflammation and ageing.					
32788422	8	55	contain	had	1217:1219	arg1	Subjects					1182:1189	Subjects	1182:1189	Subjects from developing countries	1182:1215	Subjects from developing countries had low levels of IgG galactosylation, characteristic for inflammation and ageing.					
32788422	3	56	theme	environmental	450:462	arg1	factors					464:470	both genetic and environmental factors	433:470	both genetic and environmental factors	433:470	Variability of IgG glycosylation within a population is well studied and is known to be affected by both genetic and environmental factors.					
32788422	1	57	theme	moieties	214:221	arg1	attachment					186:195	the attachment	182:195	the attachment of various sugar moieties called glycans	182:236	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	7	58	theme	IgG	990:992	arg1	monogalactosylation					994:1012	IgG monogalactosylation	990:1012	IgG monogalactosylation	990:1012	IgG monogalactosylation strongly correlated with the development level of a country, defined by United Nations health and socioeconomic development indicators, and with the expected lifespan.					
32788422	1	59	theme	structural	110:119	arg1	characteristics					121:135	structural characteristics	110:135	structural characteristics	110:135	Immunoglobulin G (IgG) is the most abundant serum antibody which structural characteristics and effector functions are modulated through the attachment of various sugar moieties called glycans.					
32788422	9	60	theme	biological	1473:1482	arg1	age					1484:1486	biological age	1473:1486	biological age	1473:1486	Our results suggest that citizens of developing countries may be exposed to environmental factors that can cause low-grade chronic inflammation and the apparent increase in biological age.					
32788422	9	61	theme	chronic	1423:1429	arg1	inflammation					1431:1442	low-grade chronic inflammation	1413:1442	low-grade chronic inflammation	1413:1442	Our results suggest that citizens of developing countries may be exposed to environmental factors that can cause low-grade chronic inflammation and the apparent increase in biological age.					
32788422	8	62	theme	IgG	1235:1237	arg1	galactosylation					1239:1253	IgG galactosylation	1235:1253	IgG galactosylation	1235:1253	Subjects from developing countries had low levels of IgG galactosylation, characteristic for inflammation and ageing.					
32788422	5	63	gly	N-glycosylation	611:625	arg1	IgG					639:641	IgG	639:641	IgG	639:641	Here we present population-specific N-glycosylation patterns of IgG, analyzed in 5 different populations totaling 10,482 IgG glycomes, and of IgG's fragment crystallizable region (Fc), analyzed in 2,579 samples from 27 populations sampled across the world.					
32753592	4	0	theme	mouse	545:549	arg1	tissues					551:557	mouse tissues	545:557	mouse tissues	545:557	The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.					
32753592	4	1	from	plasma	563:568	arg1	sulfate					532:538	heparan sulfate	524:538	heparan sulfate from mouse tissues and plasma	524:568	The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.					
32753592	4	2	from	tissues	551:557	arg1	sulfate					532:538	heparan sulfate	524:538	heparan sulfate from mouse tissues and plasma	524:568	The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.					
32753592	3	3	theme	one-pot	444:450	arg1	process					452:458	one-pot process	444:458	one-pot process	444:458	Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process.					
32753592	4	4	theme	heparan	524:530	arg1	sulfate					532:538	heparan sulfate	524:538	heparan sulfate from mouse tissues and plasma	524:568	The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.					
32753592	3	5	from	analysis	432:439	arg1	process					452:458	one-pot process	444:458	one-pot process	444:458	Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process.					
32753592	1	6	theme	Heparan	80:86	arg1	sulfate					88:94	Heparan sulfate	80:94	Heparan sulfate	80:94	Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions.					
32753592	1	6	theme	Heparan	80:86	arg1	polysaccharide					110:123	a sulfated polysaccharide	99:123	a sulfated polysaccharide that displays essential physiological functions	99:171	Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions.					
32753592	2	7	theme	heparan	305:311	arg1	sulfate					313:319	heparan sulfate	305:319	heparan sulfate	305:319	Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate.					
32753592	0	8	theme	Quantitative	0:11	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of heparan sulfate	0:39	Quantitative analysis of heparan sulfate using isotopically labeled calibrants.					
32753592	0	9	theme	heparan	25:31	arg1	sulfate					33:39	heparan sulfate	25:39	heparan sulfate	25:39	Quantitative analysis of heparan sulfate using isotopically labeled calibrants.					
32753592	1	10	theme	sulfated	101:108	arg1	sulfate					88:94	Heparan sulfate	80:94	Heparan sulfate	80:94	Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions.					
32753592	1	10	theme	sulfated	101:108	arg1	polysaccharide					110:123	a sulfated polysaccharide	99:123	a sulfated polysaccharide that displays essential physiological functions	99:171	Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions.					
32753592	2	11	theme	sulfate	313:319	arg1	composition					273:283	individual disaccharide composition	249:283	individual disaccharide composition	249:283	Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate.					
32753592	2	11	theme	sulfate	313:319	arg1	amount					295:300	total amount	289:300	total amount	289:300	Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate.					
32753592	2	11	theme	sulfate	313:319	arg1	sulfate					313:319	heparan sulfate	305:319	heparan sulfate	305:319	Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate.					
32753592	2	12	theme	LC-MS/MS-based	192:205	arg1	method					207:212	a LC-MS/MS-based method	190:212	a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate	190:319	Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate.					
32753592	2	13	dep	composition	273:283	arg1	the					245:247	the	245:247	the	245:247	Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate.					
32753592	4	14	contain	has	494:496	arg1	sensitive					480:488	sensitive	480:488	sensitive	480:488	The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.					
32753592	4	14	contain	has	494:496	arg2	throughput					502:511	the throughput	498:511	the throughput to analyze heparan sulfate from mouse tissues and plasma	498:568	The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.					
32753592	4	14	contain	has	494:496	arg1	method					465:470	The method	461:470	The method	461:470	The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.					
32753592	0	15	theme	sulfate	33:39	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of heparan sulfate	0:39	Quantitative analysis of heparan sulfate using isotopically labeled calibrants.					
32753592	2	16	theme	disaccharide	260:271	arg1	composition					273:283	individual disaccharide composition	249:283	individual disaccharide composition	249:283	Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate.					
32753592	0	17	theme	labeled	60:66	arg1	calibrants					68:77	isotopically labeled calibrants	47:77	isotopically labeled calibrants	47:77	Quantitative analysis of heparan sulfate using isotopically labeled calibrants.					
32753592	3	18	theme	disaccharide	346:357	arg1	calibrants					359:368	eight 13C-labeled disaccharide calibrants	328:368	eight 13C-labeled disaccharide calibrants	328:368	Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process.					
32753592	2	19	theme	individual	249:258	arg1	composition					273:283	individual disaccharide composition	249:283	individual disaccharide composition	249:283	Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate.					
32753592	1	20	theme	essential	139:147	arg1	functions					163:171	essential physiological functions	139:171	essential physiological functions	139:171	Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions.					
32753592	3	21	theme	13C-labeled	334:344	arg1	calibrants					359:368	eight 13C-labeled disaccharide calibrants	328:368	eight 13C-labeled disaccharide calibrants	328:368	Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process.					
32753592	1	22	theme	physiological	149:161	arg1	functions					163:171	essential physiological functions	139:171	essential physiological functions	139:171	Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions.					
32753592	3	23	theme	13C-labeled	378:388	arg1	calibrant					405:413	one 13C-labeled polysaccharide calibrant	374:413	one 13C-labeled polysaccharide calibrant	374:413	Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process.					
32753592	2	24	theme	total	289:293	arg1	amount					295:300	total amount	289:300	total amount	289:300	Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate.					
32753592	2	24	theme	total	289:293	arg1	sulfate					313:319	heparan sulfate	305:319	heparan sulfate	305:319	Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate.					
32753592	3	25	theme	polysaccharide	390:403	arg1	calibrant					405:413	one 13C-labeled polysaccharide calibrant	374:413	one 13C-labeled polysaccharide calibrant	374:413	Using eight 13C-labeled disaccharide calibrants and one 13C-labeled polysaccharide calibrant, we complete the analysis in one-pot process.					
32571193	0	0	theme	Ac-2104T	96:103	arg1	Wall					110:113	Ac-2104T Cell Wall	96:113	Ac-2104T Cell Wall	96:113	Two Glycosyl 1-Phosphate Polymers and Teichulosonic Acid from Glutamicibacter protophormiae VKM Ac-2104T Cell Wall.					
32571193	3	1	theme	-α-Kdn-	685:691	arg1	2→					693:694	→6)-β-D-Glcp-(1→8)-α-Kdn-(2→	667:694	→6)-β-D-Glcp-(1→8)-α-Kdn-(2→	667:694	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	1	theme	-α-Kdn-	685:691	arg1	polymer					541:547	the third identified polymer	520:547	the third identified polymer	520:547	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	4	2	theme	chemical	836:843	arg1	analysis					845:852	chemical analysis	836:852	chemical analysis	836:852	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	1	3	dep	protophormiae	401:413	arg1	Arthrobacter					388:399	Arthrobacter	388:399	Arthrobacter	388:399	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	4	4	theme	analysis	845:852	arg1	results					825:831	the results	821:831	the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques	821:1045	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	1	5	theme	1→6	252:254	arg1	1-phosphate					174:184	monoglycosyl 1-phosphate	161:184	monoglycosyl 1-phosphate	161:184	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	5	theme	1→6	252:254	arg1	1-P-					270:273	-6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-	187:273	1-P-	270:273	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	6	theme	cell	321:324	arg1	wall					326:329	the cell wall	317:329	the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae)	317:414	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	0	7	theme	Cell	105:108	arg1	Wall					110:113	Ac-2104T Cell Wall	96:113	Ac-2104T Cell Wall	96:113	Two Glycosyl 1-Phosphate Polymers and Teichulosonic Acid from Glutamicibacter protophormiae VKM Ac-2104T Cell Wall.					
32571193	4	8	theme	heteronuclear	973:985	arg1	techniques					1036:1045	two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques	914:1045	techniques	1036:1045	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	8	theme	heteronuclear	973:985	arg1	HSQC-TOCSY					1001:1010	HSQC-TOCSY	1001:1010	HSQC-TOCSY	1001:1010	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	8	theme	heteronuclear	973:985	arg1	HMBC					1030:1033	1H,31P HMBC	1023:1033	1H,31P HMBC	1023:1033	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	8	theme	heteronuclear	973:985	arg1	HSQC					995:998	1H,13C HSQC	988:998	1H,13C HSQC	988:998	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	8	theme	heteronuclear	973:985	arg1	HMBC					1013:1016	HMBC	1013:1016	HMBC	1013:1016	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	1	9	theme	-α-D-GlcpNAc-	256:268	arg1	1-phosphate					174:184	monoglycosyl 1-phosphate	161:184	monoglycosyl 1-phosphate	161:184	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	9	theme	-α-D-GlcpNAc-	256:268	arg1	1-P-					270:273	-6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-	187:273	1-P-	270:273	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	10	theme	-α-D-Glcp-	190:199	arg1	1-phosphate					174:184	monoglycosyl 1-phosphate	161:184	monoglycosyl 1-phosphate	161:184	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	10	theme	-α-D-Glcp-	190:199	arg1	1-P-					201:204	-6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-	187:273	1-P-	201:204	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	0	11	theme	Glycosyl	4:11	arg1	Polymers					25:32	Two Glycosyl 1-Phosphate Polymers	0:32	Two Glycosyl 1-Phosphate Polymers	0:32	Two Glycosyl 1-Phosphate Polymers and Teichulosonic Acid from Glutamicibacter protophormiae VKM Ac-2104T Cell Wall.					
32571193	3	12	from	residues	639:646	arg1	chain					660:664	the main chain	651:664	the main chain	651:664	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	13	with	polymer	541:547	arg1	Kdn					612:614	Kdn	612:614	Kdn	612:614	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	13	with	polymer	541:547	arg1	acid					606:609	3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid	555:609	3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn)	555:615	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	13	with	polymer	541:547	arg1	residues					639:646	β-D-glucopyranose residues	621:646	β-D-glucopyranose residues in the main chain	621:664	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	14	theme	actinobacteria	741:754	arg1	number					731:736	a number	729:736	a number of actinobacteria	729:754	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	4	15	theme	1H,13C	988:993	arg1	techniques					1036:1045	two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques	914:1045	techniques	1036:1045	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	15	theme	1H,13C	988:993	arg1	HSQC					995:998	1H,13C HSQC	988:998	1H,13C HSQC	988:998	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	16	dep	homonuclear	930:940	arg1	ROESY					962:966	ROESY	962:966	ROESY	962:966	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	16	dep	homonuclear	930:940	arg1	TOCSY					955:959	TOCSY	955:959	TOCSY	955:959	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	16	dep	homonuclear	930:940	arg1	COZY					949:952	1H,1H COZY	943:952	1H,1H COZY	943:952	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	3	17	theme	identified	530:539	arg1	polymer					541:547	the third identified polymer	520:547	the third identified polymer	520:547	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	17	theme	identified	530:539	arg1	acid					514:517	Teichulosonic acid	500:517	Teichulosonic acid	500:517	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	17	theme	identified	530:539	arg1	2→					693:694	→6)-β-D-Glcp-(1→8)-α-Kdn-(2→	667:694	→6)-β-D-Glcp-(1→8)-α-Kdn-(2→	667:694	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	18	from	acid	606:609	arg1	chain					660:664	the main chain	651:664	the main chain	651:664	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	0	19	theme	1-Phosphate	13:23	arg1	Polymers					25:32	Two Glycosyl 1-Phosphate Polymers	0:32	Two Glycosyl 1-Phosphate Polymers	0:32	Two Glycosyl 1-Phosphate Polymers and Teichulosonic Acid from Glutamicibacter protophormiae VKM Ac-2104T Cell Wall.					
32571193	3	20	theme	main	655:658	arg1	chain					660:664	the main chain	651:664	the main chain	651:664	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	0	21	dep	Glutamicibacter	62:76	arg1	protophormiae					78:90	Glutamicibacter protophormiae	62:90	Glutamicibacter protophormiae	62:90	Two Glycosyl 1-Phosphate Polymers and Teichulosonic Acid from Glutamicibacter protophormiae VKM Ac-2104T Cell Wall.					
32571193	0	22	theme	Teichulosonic	38:50	arg1	Acid					52:55	Teichulosonic Acid	38:55	Teichulosonic Acid from Glutamicibacter protophormiae	38:90	Two Glycosyl 1-Phosphate Polymers and Teichulosonic Acid from Glutamicibacter protophormiae VKM Ac-2104T Cell Wall.					
32571193	4	23	theme	NMR	891:893	arg1	spectroscopy					895:906	31P NMR spectroscopy	887:906	31P NMR spectroscopy	887:906	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	1	24	theme	Ac-2104T	368:375	arg1	wall					326:329	the cell wall	317:329	the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae)	317:414	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	4	25	theme	glycopolymers	781:793	arg1	structures					761:770	The structures	757:770	The structures of these glycopolymers	757:793	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	1	26	theme	diglycosyl	211:220	arg1	1-phosphate					174:184	monoglycosyl 1-phosphate	161:184	monoglycosyl 1-phosphate	161:184	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	26	theme	diglycosyl	211:220	arg1	1-P-					270:273	-6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-	187:273	1-P-	270:273	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	4	27	theme	31P	887:889	arg1	spectroscopy					895:906	31P NMR spectroscopy	887:906	31P NMR spectroscopy	887:906	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	2	28	from	time	479:482	arg1	prokaryotes					487:497	prokaryotes	487:497	prokaryotes	487:497	The structures of these polymers were described for the first time in prokaryotes.					
32571193	1	29	theme	1-phosphate	222:232	arg1	1-phosphate					174:184	monoglycosyl 1-phosphate	161:184	monoglycosyl 1-phosphate	161:184	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	29	theme	1-phosphate	222:232	arg1	1-P-					270:273	-6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-	187:273	1-P-	270:273	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	2	30	theme	polymers	441:448	arg1	structures					421:430	The structures	417:430	The structures of these polymers	417:448	The structures of these polymers were described for the first time in prokaryotes.					
32571193	1	31	theme	repeating	283:291	arg1	unit					293:296	the repeating unit	279:296	the repeating unit	279:296	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	4	32	theme	1H	874:875	arg1	results					825:831	the results	821:831	the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques	821:1045	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	0	33	from	Glutamicibacter	62:76	arg1	Polymers					25:32	Two Glycosyl 1-Phosphate Polymers	0:32	Two Glycosyl 1-Phosphate Polymers	0:32	Two Glycosyl 1-Phosphate Polymers and Teichulosonic Acid from Glutamicibacter protophormiae VKM Ac-2104T Cell Wall.					
32571193	0	33	from	Glutamicibacter	62:76	arg1	Acid					52:55	Teichulosonic Acid	38:55	Teichulosonic Acid from Glutamicibacter protophormiae	38:90	Two Glycosyl 1-Phosphate Polymers and Teichulosonic Acid from Glutamicibacter protophormiae VKM Ac-2104T Cell Wall.					
32571193	3	34	theme	3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic	555:604	arg1	Kdn					612:614	Kdn	612:614	Kdn	612:614	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	34	theme	3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic	555:604	arg1	acid					606:609	3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid	555:609	3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn)	555:615	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	4	35	dep	techniques	1036:1045	arg1	HSQC-TOCSY					1001:1010	HSQC-TOCSY	1001:1010	HSQC-TOCSY	1001:1010	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	35	dep	techniques	1036:1045	arg1	techniques					1036:1045	two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques	914:1045	techniques	1036:1045	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	35	dep	techniques	1036:1045	arg1	HMBC					1030:1033	1H,31P HMBC	1023:1033	1H,31P HMBC	1023:1033	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	35	dep	techniques	1036:1045	arg1	HSQC					995:998	1H,13C HSQC	988:998	1H,13C HSQC	988:998	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	35	dep	techniques	1036:1045	arg1	HMBC					1013:1016	HMBC	1013:1016	HMBC	1013:1016	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	1	36	theme	glycosyl	120:127	arg1	polymers					141:148	Two glycosyl 1-phosphate polymers	116:148	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit	116:296	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	3	37	theme	1→8	681:683	arg1	2→					693:694	→6)-β-D-Glcp-(1→8)-α-Kdn-(2→	667:694	→6)-β-D-Glcp-(1→8)-α-Kdn-(2→	667:694	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	37	theme	1→8	681:683	arg1	polymer					541:547	the third identified polymer	520:547	the third identified polymer	520:547	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	38	located	detected	717:724	arg1	number					731:736	a number	729:736	a number of actinobacteria	729:754	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	38	located	detected	717:724	arg2	polymer					541:547	the third identified polymer	520:547	the third identified polymer	520:547	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	38	located	detected	717:724	arg2	acid					514:517	Teichulosonic acid	500:517	Teichulosonic acid	500:517	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	1	39	theme	1-phosphate	129:139	arg1	polymers					141:148	Two glycosyl 1-phosphate polymers	116:148	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit	116:296	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	4	40	theme	one-dimensional	858:872	arg1	1H					874:875	one-dimensional 1H	858:875	one-dimensional 1H	858:875	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	3	41	theme	-β-D-Glcp-	670:679	arg1	2→					693:694	→6)-β-D-Glcp-(1→8)-α-Kdn-(2→	667:694	→6)-β-D-Glcp-(1→8)-α-Kdn-(2→	667:694	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	41	theme	-β-D-Glcp-	670:679	arg1	polymer					541:547	the third identified polymer	520:547	the third identified polymer	520:547	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	42	theme	β-D-glucopyranose	621:637	arg1	residues					639:646	β-D-glucopyranose residues	621:646	β-D-glucopyranose residues in the main chain	621:664	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	4	43	theme	1H,31P	1023:1028	arg1	techniques					1036:1045	two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques	914:1045	techniques	1036:1045	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	43	theme	1H,31P	1023:1028	arg1	HMBC					1030:1033	1H,31P HMBC	1023:1033	1H,31P HMBC	1023:1033	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	44	theme	1H,1H	943:947	arg1	ROESY					962:966	ROESY	962:966	ROESY	962:966	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	44	theme	1H,1H	943:947	arg1	TOCSY					955:959	TOCSY	955:959	TOCSY	955:959	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	4	44	theme	1H,1H	943:947	arg1	COZY					949:952	1H,1H COZY	943:952	1H,1H COZY	943:952	The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional 1H, 13C, and 31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and 1H,31P HMBC) techniques.					
32571193	1	45	theme	monoglycosyl	161:172	arg1	1-phosphate					174:184	monoglycosyl 1-phosphate	161:184	monoglycosyl 1-phosphate	161:184	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	45	theme	monoglycosyl	161:172	arg1	1-P-					201:204	-6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-	187:273	1-P-	201:204	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	45	theme	monoglycosyl	161:172	arg1	1-P-					270:273	-6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-	187:273	1-P-	270:273	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	2	46	theme	first	473:477	arg1	time					479:482	the first time	469:482	the first time in prokaryotes	469:497	The structures of these polymers were described for the first time in prokaryotes.					
32571193	1	47	contain	containing	150:159	arg1	polymers					141:148	Two glycosyl 1-phosphate polymers	116:148	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit	116:296	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	47	contain	containing	150:159	arg2	1-phosphate					174:184	monoglycosyl 1-phosphate	161:184	monoglycosyl 1-phosphate	161:184	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	47	contain	containing	150:159	arg2	1-P-					201:204	-6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-	187:273	1-P-	201:204	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	47	contain	containing	150:159	arg2	1-P-					270:273	-6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-	187:273	1-P-	270:273	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	3	48	theme	Teichulosonic	500:512	arg1	polymer					541:547	the third identified polymer	520:547	the third identified polymer	520:547	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	3	48	theme	Teichulosonic	500:512	arg1	acid					514:517	Teichulosonic acid	500:517	Teichulosonic acid	500:517	Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero-α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1→8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria.					
32571193	1	49	theme	-α-D-GalpNAc-	238:250	arg1	1-phosphate					174:184	monoglycosyl 1-phosphate	161:184	monoglycosyl 1-phosphate	161:184	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32571193	1	49	theme	-α-D-GalpNAc-	238:250	arg1	1-P-					270:273	-6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-	187:273	1-P-	270:273	Two glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)-α-D-Glcp-(1-P-, and diglycosyl 1-phosphate, -6)-α-D-GalpNAc-(1→6)-α-D-GlcpNAc-(1-P-, in the repeating unit were identified in the cell wall of Glutamicibacter protophormiae VKM Ac-2104T (formerly, Arthrobacter protophormiae).					
32396713	0	0	theme	Glycosyltransferases	80:99	arg1	Characterization					54:69	Characterization	54:69	Characterization	54:69	A Glycan Array-Based Assay for the Identification and Characterization of Plant Glycosyltransferases.					
32396713	0	0	theme	Glycosyltransferases	80:99	arg1	Identification					35:48	Identification	35:48	Identification	35:48	A Glycan Array-Based Assay for the Identification and Characterization of Plant Glycosyltransferases.					
32396713	1	1	theme	promising	159:167	arg1	plants					110:115	Growing plants	102:115	Growing plants with modified cell wall compositions	102:152	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	1	1	theme	promising	159:167	arg1	strategy					169:176	a promising strategy	157:176	a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties	157:280	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	1	2	theme	other	255:259	arg1	properties					271:280	other important properties	255:280	other important properties	255:280	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	0	3	theme	Plant	74:78	arg1	Glycosyltransferases					80:99	Plant Glycosyltransferases	74:99	Plant Glycosyltransferases	74:99	A Glycan Array-Based Assay for the Identification and Characterization of Plant Glycosyltransferases.					
32396713	2	4	theme	wall	352:355	arg1	prerequisite					389:400	a prerequisite	387:400	a prerequisite	387:400	In order to alter biomass architecture, a detailed knowledge of cell wall structure and biosynthesis is a prerequisite.					
32396713	2	4	theme	wall	352:355	arg1	knowledge					334:342	a detailed knowledge	323:342	a detailed knowledge of cell wall structure and biosynthesis	323:382	In order to alter biomass architecture, a detailed knowledge of cell wall structure and biosynthesis is a prerequisite.					
32396713	1	5	theme	important	261:269	arg1	properties					271:280	other important properties	255:280	other important properties	255:280	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	5	6	theme	cell	1106:1109	arg1	research					1129:1136	plant cell wall biosynthesis research	1100:1136	plant cell wall biosynthesis research	1100:1136	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	4	7	theme	transferred	806:816	arg1	monosaccharides					818:832	the transferred monosaccharides	802:832	the transferred monosaccharides	802:832	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	2	8	dep	wall	352:355	arg1	structure					357:365	structure	357:365	structure	357:365	In order to alter biomass architecture, a detailed knowledge of cell wall structure and biosynthesis is a prerequisite.					
32396713	2	8	dep	wall	352:355	arg1	biosynthesis					371:382	biosynthesis	371:382	biosynthesis	371:382	In order to alter biomass architecture, a detailed knowledge of cell wall structure and biosynthesis is a prerequisite.					
32396713	3	9	theme	wall	519:522	arg1	GTs					559:561	GTs	559:561	GTs	559:561	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	3	9	theme	wall	519:522	arg1	glycosyltransferases					537:556	plant cell wall biosynthetic glycosyltransferases	508:556	plant cell wall biosynthetic glycosyltransferases (GTs)	508:562	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	5	10	theme	GTs	1011:1013	arg1	combinations					986:997	combinations	986:997	combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors	986:1069	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	4	11	theme	wall	758:761	arg1	oligosaccharides					763:778	selected synthetic plant cell wall oligosaccharides	728:778	selected synthetic plant cell wall oligosaccharides on the array	728:791	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	2	12	theme	cell	347:350	arg1	wall					352:355	cell wall structure and biosynthesis	347:382	cell wall structure and biosynthesis	347:382	In order to alter biomass architecture, a detailed knowledge of cell wall structure and biosynthesis is a prerequisite.					
32396713	5	13	theme	oligosaccharide	1045:1059	arg1	acceptors					1061:1069	oligosaccharide acceptors	1045:1069	oligosaccharide acceptors	1045:1069	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	4	14	theme	synthetic	737:745	arg1	oligosaccharides					763:778	selected synthetic plant cell wall oligosaccharides	728:778	selected synthetic plant cell wall oligosaccharides on the array	728:791	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	5	15	theme	donors	1033:1038	arg1	combinations					986:997	combinations	986:997	combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors	986:1069	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	5	16	theme	acceptors	1061:1069	arg1	combinations					986:997	combinations	986:997	combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors	986:1069	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	0	17	theme	Glycan	2:7	arg1	Assay					21:25	A Glycan Array-Based Assay	0:25	A Glycan Array-Based Assay for the Identification and Characterization of Plant Glycosyltransferases	0:99	A Glycan Array-Based Assay for the Identification and Characterization of Plant Glycosyltransferases.					
32396713	4	18	theme	different	585:593	arg1	galactosyl-					620:630	galactosyl-	620:630	galactosyl-	620:630	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	4	19	dep	different	585:593	arg1	expressed					610:618	expressed	610:618	expressed	610:618	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	3	20	theme	glycan	420:425	arg1	assay					439:443	a glycan array-based assay	418:443	a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs)	418:562	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	4	21	theme	alkynyl-modified	910:925	arg1	dye					927:929	an alkynyl-modified dye	907:929	an alkynyl-modified dye	907:929	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	1	22	theme	Growing	102:108	arg1	plants					110:115	Growing plants	102:115	Growing plants with modified cell wall compositions	102:152	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	1	22	theme	Growing	102:108	arg1	strategy					169:176	a promising strategy	157:176	a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties	157:280	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	3	23	theme	array-based	427:437	arg1	assay					439:443	a glycan array-based assay	418:443	a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs)	418:562	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	5	24	theme	sugar	1027:1031	arg1	donors					1033:1038	nucleotide sugar donors	1016:1038	nucleotide sugar donors	1016:1038	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	4	25	theme	1,3-dipolar	867:877	arg1	reaction					893:900	a 1,3-dipolar cycloaddition reaction	865:900	a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye	865:929	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	4	26	theme	azido-functionalized	680:699	arg1	donors					718:723	azido-functionalized sugar nucleotide donors	680:723	azido-functionalized sugar nucleotide donors	680:723	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	0	27	theme	Array-Based	9:19	arg1	Assay					21:25	A Glycan Array-Based Assay	0:25	A Glycan Array-Based Assay for the Identification and Characterization of Plant Glycosyltransferases	0:99	A Glycan Array-Based Assay for the Identification and Characterization of Plant Glycosyltransferases.					
32396713	5	28	theme	nucleotide	1016:1025	arg1	donors					1033:1038	nucleotide sugar donors	1016:1038	nucleotide sugar donors	1016:1038	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	2	29	theme	biomass	301:307	arg1	architecture					309:320	biomass architecture	301:320	biomass architecture	301:320	In order to alter biomass architecture, a detailed knowledge of cell wall structure and biosynthesis is a prerequisite.					
32396713	4	30	theme	cell	753:756	arg1	oligosaccharides					763:778	selected synthetic plant cell wall oligosaccharides	728:778	selected synthetic plant cell wall oligosaccharides on the array	728:791	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	3	31	theme	cell	514:517	arg1	GTs					559:561	GTs	559:561	GTs	559:561	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	3	31	theme	cell	514:517	arg1	glycosyltransferases					537:556	plant cell wall biosynthetic glycosyltransferases	508:556	plant cell wall biosynthetic glycosyltransferases (GTs)	508:562	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	4	32	theme	sugar	701:705	arg1	donors					718:723	azido-functionalized sugar nucleotide donors	680:723	azido-functionalized sugar nucleotide donors	680:723	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	4	33	theme	selected	728:735	arg1	oligosaccharides					763:778	selected synthetic plant cell wall oligosaccharides	728:778	selected synthetic plant cell wall oligosaccharides on the array	728:791	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	4	34	theme	nucleotide	707:716	arg1	donors					718:723	azido-functionalized sugar nucleotide donors	680:723	azido-functionalized sugar nucleotide donors	680:723	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	0	35	dep	Identification	35:48	arg1	the					31:33	the	31:33	the	31:33	A Glycan Array-Based Assay for the Identification and Characterization of Plant Glycosyltransferases.					
32396713	1	36	theme	biomass	223:229	arg1	digestibility					231:243	biomass digestibility	223:243	biomass digestibility	223:243	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	5	37	theme	biosynthesis	1116:1127	arg1	research					1129:1136	plant cell wall biosynthesis research	1100:1136	plant cell wall biosynthesis research	1100:1136	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	3	38	theme	plant	508:512	arg1	GTs					559:561	GTs	559:561	GTs	559:561	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	3	38	theme	plant	508:512	arg1	glycosyltransferases					537:556	plant cell wall biosynthetic glycosyltransferases	508:556	plant cell wall biosynthetic glycosyltransferases (GTs)	508:562	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	1	39	theme	modified	122:129	arg1	compositions					141:152	modified cell wall compositions	122:152	modified cell wall compositions	122:152	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	4	40	theme	cycloaddition	879:891	arg1	reaction					893:900	a 1,3-dipolar cycloaddition reaction	865:900	a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye	865:929	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	3	41	theme	biosynthetic	524:535	arg1	GTs					559:561	GTs	559:561	GTs	559:561	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	3	41	theme	biosynthetic	524:535	arg1	glycosyltransferases					537:556	plant cell wall biosynthetic glycosyltransferases	508:556	plant cell wall biosynthetic glycosyltransferases (GTs)	508:562	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	1	42	theme	cell	131:134	arg1	compositions					141:152	modified cell wall compositions	122:152	modified cell wall compositions	122:152	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	5	43	theme	wall	1111:1114	arg1	research					1129:1136	plant cell wall biosynthesis research	1100:1136	plant cell wall biosynthesis research	1100:1136	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	4	44	theme	chip	856:859	arg1	"					860:860	chip"	856:860	on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye	853:929	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	4	45	theme	plant	747:751	arg1	oligosaccharides					763:778	selected synthetic plant cell wall oligosaccharides	728:778	selected synthetic plant cell wall oligosaccharides on the array	728:791	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	1	46	theme	wall	136:139	arg1	compositions					141:152	modified cell wall compositions	122:152	modified cell wall compositions	122:152	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	4	47	with	reaction	893:900	arg1	dye					927:929	an alkynyl-modified dye	907:929	an alkynyl-modified dye	907:929	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	5	48	theme	combinations	986:997	arg1	thousands					973:981	thousands	973:981	thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors	973:1069	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	4	49	theme	on	853:854	arg1	"					860:860	chip"	856:860	on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye	853:929	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
32396713	2	50	theme	detailed	325:332	arg1	prerequisite					389:400	a prerequisite	387:400	a prerequisite	387:400	In order to alter biomass architecture, a detailed knowledge of cell wall structure and biosynthesis is a prerequisite.					
32396713	2	50	theme	detailed	325:332	arg1	knowledge					334:342	a detailed knowledge	323:342	a detailed knowledge of cell wall structure and biosynthesis	323:382	In order to alter biomass architecture, a detailed knowledge of cell wall structure and biosynthesis is a prerequisite.					
32396713	5	51	theme	putative	1002:1009	arg1	GTs					1011:1013	putative GTs	1002:1013	putative GTs	1002:1013	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	3	52	theme	high-throughput	453:467	arg1	identification					469:482	high-throughput identification	453:482	high-throughput identification	453:482	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	3	53	dep	identification	469:482	arg1	the					449:451	the	449:451	the	449:451	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	3	54	theme	glycosyltransferases	537:556	arg1	characterization					488:503	characterization	488:503	characterization	488:503	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	3	54	theme	glycosyltransferases	537:556	arg1	identification					469:482	high-throughput identification	453:482	high-throughput identification	453:482	We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs).					
32396713	5	55	theme	plant	1100:1104	arg1	research					1129:1136	plant cell wall biosynthesis research	1100:1136	plant cell wall biosynthesis research	1100:1136	The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.					
32396713	1	56	with	plants	110:115	arg1	compositions					141:152	modified cell wall compositions	122:152	modified cell wall compositions	122:152	Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties.					
32396713	4	57	from	oligosaccharides	763:778	arg1	array					787:791	the array	783:791	the array	783:791	We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized "on chip" by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye.					
34834161	6	0	theme	appropriate	1181:1191	arg1	confirmation					1193:1204	appropriate confirmation	1181:1204	appropriate confirmation of glycan occupancy	1181:1224	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	1	1	theme	mAb	286:288	arg1	derivatives					290:300	mAb derivatives	286:300	mAb derivatives	286:300	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	11	2	theme	antibodies	2296:2305	arg1	development					2269:2279	the development	2265:2279	the development of "biobetter" antibodies	2265:2305	Our findings shed light into mAb glycobiology and potential implications in the application of this technique for the development of "biobetter" antibodies.					
34834161	4	3	gly	N-glycosylation	722:736	arg2	sequons					738:744	N-glycosylation sequons	722:744	N-glycosylation sequons	722:744	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	9	4	theme	higher	1809:1814	arg1	accessibility					1816:1828	higher accessibility	1809:1828	higher accessibility to enzymes responsible for glycan maturation	1809:1873	These findings suggest that Fab N-glycosites have higher accessibility to enzymes responsible for glycan maturation.					
34834161	6	5	theme	profile	1280:1286	arg1	confirmation					1193:1204	appropriate confirmation	1181:1204	appropriate confirmation of glycan occupancy	1181:1224	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	6	5	theme	profile	1280:1286	arg1	characterization					1241:1256	structural characterization	1230:1256	structural characterization of the ensuing glycan profile	1230:1286	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	2	6	theme	Antibody	303:310	arg1	aggregates					312:321	Antibody aggregates	303:321	Antibody aggregates	303:321	Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity of the antibody monomer to bind to its cognate antigen.					
34834161	4	7	from	insertion	709:717	arg1	acids					755:759	amino acids	749:759	amino acids proximal to an aggregation-prone region	749:799	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	4	8	theme	monomers	919:926	arg1	self-association					890:905	self-association	890:905	self-association of antibody monomers	890:926	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	6	9	theme	ensuing	1265:1271	arg1	profile					1280:1286	the ensuing glycan profile	1261:1286	the ensuing glycan profile	1261:1286	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	11	10	from	glycobiology	2184:2195	arg1	application					2231:2241	the application	2227:2241	the application of this technique	2227:2259	Our findings shed light into mAb glycobiology and potential implications in the application of this technique for the development of "biobetter" antibodies.					
34834161	4	11	theme	N-glycosylation	722:736	arg1	sequons					738:744	N-glycosylation sequons	722:744	N-glycosylation sequons	722:744	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	1	12	theme	clinical	226:233	arg1	use					235:237	clinical use	226:237	clinical use	226:237	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	7	13	gly	N-glycosylation	1465:1479	arg1	mutants					1496:1502	the mutants	1492:1502	the mutants	1492:1502	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	9	14	theme	responsible	1841:1851	arg1	enzymes					1833:1839	enzymes	1833:1839	enzymes responsible for glycan maturation	1833:1873	These findings suggest that Fab N-glycosites have higher accessibility to enzymes responsible for glycan maturation.					
34834161	8	15	contain	contains	1660:1667	arg2	site					1694:1697	a single N-glycosylation site	1669:1697	a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region	1669:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	8	15	contain	contains	1660:1667	arg1	antibody					1644:1651	the HEK Trastuzumab antibody	1624:1651	the HEK Trastuzumab antibody	1624:1651	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	3	16	theme	aggregation-prone	610:626	arg1	APR					637:639	APR	637:639	APR	637:639	A common strategy to tackle protein aggregation involves the identification of surface-exposed aggregation-prone regions (APR) for replacement through protein engineering.					
34834161	3	16	theme	aggregation-prone	610:626	arg1	regions					628:634	surface-exposed aggregation-prone regions	594:634	surface-exposed aggregation-prone regions (APR) for replacement	594:656	A common strategy to tackle protein aggregation involves the identification of surface-exposed aggregation-prone regions (APR) for replacement through protein engineering.					
34834161	9	17	theme	glycan	1857:1862	arg1	maturation					1864:1873	glycan maturation	1857:1873	glycan maturation	1857:1873	These findings suggest that Fab N-glycosites have higher accessibility to enzymes responsible for glycan maturation.					
34834161	8	18	theme	higher	1580:1585	arg1	heterogeneity					1598:1610	significantly higher structural heterogeneity	1566:1610	significantly higher structural heterogeneity	1566:1610	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	10	19	theme	accelerated	1962:1972	arg1	studies					1974:1980	accelerated studies	1962:1980	accelerated studies	1962:1980	Further, we have studied effects on additional glycosylation on protein stability via accelerated studies by following protein folding and aggregation propensities and observed that additional glycosylation indeed enhances physical stability and prevent protein aggregation.					
34834161	1	20	theme	monoclonal	254:263	arg1	mAb					277:279	mAb	277:279	mAb	277:279	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	1	20	theme	monoclonal	254:263	arg1	antibodies					265:274	therapeutic monoclonal antibodies	242:274	therapeutic monoclonal antibodies (mAb)	242:280	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	11	21	theme	biobetter	2285:2293	arg1	antibodies					2296:2305	"biobetter" antibodies	2284:2305	"biobetter" antibodies	2284:2305	Our findings shed light into mAb glycobiology and potential implications in the application of this technique for the development of "biobetter" antibodies.					
34834161	8	22	with	profile	1553:1559	arg1	heterogeneity					1598:1610	significantly higher structural heterogeneity	1566:1610	significantly higher structural heterogeneity	1566:1610	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	1	23	theme	Protein	146:152	arg1	aggregation					154:164	Protein aggregation	146:164	Protein aggregation	146:164	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	4	24	theme	aggregation-prone	776:792	arg1	region					794:799	an aggregation-prone region	773:799	an aggregation-prone region	773:799	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	10	25	theme	protein	1995:2001	arg1	folding					2003:2009	protein folding	1995:2009	protein folding	1995:2009	Further, we have studied effects on additional glycosylation on protein stability via accelerated studies by following protein folding and aggregation propensities and observed that additional glycosylation indeed enhances physical stability and prevent protein aggregation.					
34834161	5	26	theme	full-size	988:996	arg1	adalimumab					998:1007	full-size adalimumab	988:1007	full-size adalimumab	988:1007	We recently implemented this approach in the Fab region of full-size adalimumab and demonstrated that the thermodynamic stability of the Fab domain increases upon N-glycosite addition.					
34834161	8	27	theme	HEK	1628:1630	arg1	antibody					1644:1651	the HEK Trastuzumab antibody	1624:1651	the HEK Trastuzumab antibody	1624:1651	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	7	28	theme	detailed	1456:1463	arg1	profile					1481:1487	a detailed N-glycosylation profile	1454:1487	a detailed N-glycosylation profile of the mutants	1454:1502	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	0	29	theme	Added	66:70	arg1	Sequons					86:92	Rationally Added Glycosylation Sequons	55:92	Rationally Added Glycosylation Sequons	55:92	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity.					
34834161	3	30	theme	protein	666:672	arg1	engineering					674:684	protein engineering	666:684	protein engineering	666:684	A common strategy to tackle protein aggregation involves the identification of surface-exposed aggregation-prone regions (APR) for replacement through protein engineering.					
34834161	1	31	theme	recurring	180:188	arg1	complication					190:201	a recurring complication	178:201	a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives	178:300	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	7	32	theme	candidate	1330:1338	arg1	positions					1340:1348	previously identified candidate positions	1308:1348	previously identified candidate positions on the Fab domain of Trastuzumab	1308:1381	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	8	33	theme	Trastuzumab	1632:1642	arg1	antibody					1644:1651	the HEK Trastuzumab antibody	1624:1651	the HEK Trastuzumab antibody	1624:1651	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	1	34	from	complication	190:201	arg1	manufacture					210:220	manufacture	210:220	manufacture	210:220	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	1	34	from	complication	190:201	arg1	use					235:237	clinical use	226:237	clinical use	226:237	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	5	35	theme	thermodynamic	1035:1047	arg1	stability					1049:1057	the thermodynamic stability	1031:1057	the thermodynamic stability of the Fab domain	1031:1075	We recently implemented this approach in the Fab region of full-size adalimumab and demonstrated that the thermodynamic stability of the Fab domain increases upon N-glycosite addition.					
34834161	9	36	contain	have	1804:1807	arg1	N-glycosites					1791:1802	Fab N-glycosites	1787:1802	Fab N-glycosites	1787:1802	These findings suggest that Fab N-glycosites have higher accessibility to enzymes responsible for glycan maturation.					
34834161	9	36	contain	have	1804:1807	arg2	accessibility					1816:1828	higher accessibility	1809:1828	higher accessibility to enzymes responsible for glycan maturation	1809:1873	These findings suggest that Fab N-glycosites have higher accessibility to enzymes responsible for glycan maturation.					
34834161	0	37	theme	Glycan	127:132	arg1	Complexity					134:143	Significantly Increased Glycan Complexity	103:143	Significantly Increased Glycan Complexity	103:143	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity.					
34834161	5	38	gly	N-glycosite	1092:1102	arg2	N-glycosite					1092:1102	N-glycosite addition	1092:1111	N-glycosite addition	1092:1111	We recently implemented this approach in the Fab region of full-size adalimumab and demonstrated that the thermodynamic stability of the Fab domain increases upon N-glycosite addition.					
34834161	8	39	theme	N-glycosylation	1678:1692	arg1	site					1694:1697	a single N-glycosylation site	1669:1697	a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region	1669:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	2	40	theme	product	427:433	arg1	shelf-life					394:403	the shelf-life	390:403	the shelf-life of the pharmaceutical product	390:433	Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity of the antibody monomer to bind to its cognate antigen.					
34834161	0	41	theme	Glycan	0:5	arg1	Analysis					15:22	Glycan Profile Analysis	0:22	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons	0:92	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity.					
34834161	2	42	theme	monomer	475:481	arg1	capacity					450:457	the capacity	446:457	the capacity of the antibody monomer to bind to its cognate antigen	446:512	Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity of the antibody monomer to bind to its cognate antigen.					
34834161	6	43	theme	experimental	1123:1134	arg1	data					1136:1139	Previous experimental data	1114:1139	Previous experimental data reported for this technique	1114:1167	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	0	44	theme	Engineered	27:36	arg1	Trastuzumab					38:48	Engineered Trastuzumab	27:48	Engineered Trastuzumab with Rationally Added Glycosylation Sequons	27:92	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity.					
34834161	5	45	theme	N-glycosite	1092:1102	arg1	addition					1104:1111	N-glycosite addition	1092:1111	N-glycosite addition	1092:1111	We recently implemented this approach in the Fab region of full-size adalimumab and demonstrated that the thermodynamic stability of the Fab domain increases upon N-glycosite addition.					
34834161	6	46	theme	Previous	1114:1121	arg1	data					1136:1139	Previous experimental data	1114:1139	Previous experimental data reported for this technique	1114:1167	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	3	47	theme	common	517:522	arg1	strategy					524:531	A common strategy	515:531	A common strategy to tackle protein aggregation	515:561	A common strategy to tackle protein aggregation involves the identification of surface-exposed aggregation-prone regions (APR) for replacement through protein engineering.					
34834161	7	48	theme	tandem	1396:1401	arg1	spectrometry					1408:1419	tandem mass spectrometry	1396:1419	tandem mass spectrometry	1396:1419	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	8	49	theme	CH2	1730:1732	arg1	region					1751:1756	the Fc region	1744:1756	the Fc region	1744:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	8	49	theme	CH2	1730:1732	arg1	domain					1734:1739	the CH2 domain	1726:1739	the CH2 domain of the Fc region	1726:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	10	50	theme	additional	2058:2067	arg1	glycosylation					2069:2081	additional glycosylation	2058:2081	additional glycosylation	2058:2081	Further, we have studied effects on additional glycosylation on protein stability via accelerated studies by following protein folding and aggregation propensities and observed that additional glycosylation indeed enhances physical stability and prevent protein aggregation.					
34834161	1	51	theme	antibodies	265:274	arg1	manufacture					210:220	manufacture	210:220	manufacture	210:220	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	1	51	theme	antibodies	265:274	arg1	use					235:237	clinical use	226:237	clinical use	226:237	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	11	52	theme	potential	2201:2209	arg1	implications					2211:2222	potential implications	2201:2222	potential implications in the application of this technique for the development of "biobetter" antibodies	2201:2305	Our findings shed light into mAb glycobiology and potential implications in the application of this technique for the development of "biobetter" antibodies.					
34834161	5	53	theme	Fab	1066:1068	arg1	domain					1070:1075	the Fab domain	1062:1075	the Fab domain	1062:1075	We recently implemented this approach in the Fab region of full-size adalimumab and demonstrated that the thermodynamic stability of the Fab domain increases upon N-glycosite addition.					
34834161	6	54	theme	glycan	1209:1214	arg1	occupancy					1216:1224	glycan occupancy	1209:1224	glycan occupancy	1209:1224	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	3	55	theme	protein	543:549	arg1	aggregation					551:561	protein aggregation	543:561	protein aggregation	543:561	A common strategy to tackle protein aggregation involves the identification of surface-exposed aggregation-prone regions (APR) for replacement through protein engineering.					
34834161	9	56	gly	N-glycosites	1791:1802	arg2	N-glycosites					1791:1802	Fab N-glycosites	1787:1802	Fab N-glycosites	1787:1802	These findings suggest that Fab N-glycosites have higher accessibility to enzymes responsible for glycan maturation.					
34834161	8	57	theme	Fc	1748:1749	arg1	region					1751:1756	the Fc region	1744:1756	the Fc region	1744:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	10	58	theme	physical	2099:2106	arg1	stability					2108:2116	physical stability	2099:2116	physical stability	2099:2116	Further, we have studied effects on additional glycosylation on protein stability via accelerated studies by following protein folding and aggregation propensities and observed that additional glycosylation indeed enhances physical stability and prevent protein aggregation.					
34834161	8	59	from	located	1715:1721	arg1	region					1751:1756	the Fc region	1744:1756	the Fc region	1744:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	8	59	from	located	1715:1721	arg1	domain					1734:1739	the CH2 domain	1726:1739	the CH2 domain of the Fc region	1726:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	9	60	theme	Fab	1787:1789	arg1	N-glycosites					1791:1802	Fab N-glycosites	1787:1802	Fab N-glycosites	1787:1802	These findings suggest that Fab N-glycosites have higher accessibility to enzymes responsible for glycan maturation.					
34834161	7	61	theme	Fab	1357:1359	arg1	domain					1361:1366	the Fab domain	1353:1366	the Fab domain of Trastuzumab	1353:1381	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	7	61	theme	Fab	1357:1359	arg1	Trastuzumab					1371:1381	Trastuzumab	1371:1381	Trastuzumab	1371:1381	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	11	62	from	implications	2211:2222	arg1	application					2231:2241	the application	2227:2241	the application of this technique	2227:2259	Our findings shed light into mAb glycobiology and potential implications in the application of this technique for the development of "biobetter" antibodies.					
34834161	1	63	theme	derivatives	290:300	arg1	manufacture					210:220	manufacture	210:220	manufacture	210:220	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	1	63	theme	derivatives	290:300	arg1	use					235:237	clinical use	226:237	clinical use	226:237	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	10	64	theme	protein	1940:1946	arg1	stability					1948:1956	protein stability	1940:1956	protein stability	1940:1956	Further, we have studied effects on additional glycosylation on protein stability via accelerated studies by following protein folding and aggregation propensities and observed that additional glycosylation indeed enhances physical stability and prevent protein aggregation.					
34834161	10	65	theme	protein	2130:2136	arg1	aggregation					2138:2148	protein aggregation	2130:2148	protein aggregation	2130:2148	Further, we have studied effects on additional glycosylation on protein stability via accelerated studies by following protein folding and aggregation propensities and observed that additional glycosylation indeed enhances physical stability and prevent protein aggregation.					
34834161	8	66	from	domain	1734:1739	arg1	located					1715:1721	located	1715:1721	located	1715:1721	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	10	67	dep	folding	2003:2009	arg1	propensities					2027:2038	propensities	2027:2038	propensities	2027:2038	Further, we have studied effects on additional glycosylation on protein stability via accelerated studies by following protein folding and aggregation propensities and observed that additional glycosylation indeed enhances physical stability and prevent protein aggregation.					
34834161	6	68	theme	glycan	1273:1278	arg1	profile					1280:1286	the ensuing glycan profile	1261:1286	the ensuing glycan profile	1261:1286	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	3	69	theme	regions	628:634	arg1	identification					576:589	the identification	572:589	the identification of surface-exposed aggregation-prone regions (APR) for replacement	572:656	A common strategy to tackle protein aggregation involves the identification of surface-exposed aggregation-prone regions (APR) for replacement through protein engineering.					
34834161	4	70	theme	antibody	910:917	arg1	monomers					919:926	antibody monomers	910:926	antibody monomers	910:926	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	3	71	theme	surface-exposed	594:608	arg1	APR					637:639	APR	637:639	APR	637:639	A common strategy to tackle protein aggregation involves the identification of surface-exposed aggregation-prone regions (APR) for replacement through protein engineering.					
34834161	3	71	theme	surface-exposed	594:608	arg1	regions					628:634	surface-exposed aggregation-prone regions	594:634	surface-exposed aggregation-prone regions (APR) for replacement	594:656	A common strategy to tackle protein aggregation involves the identification of surface-exposed aggregation-prone regions (APR) for replacement through protein engineering.					
34834161	8	72	theme	structural	1587:1596	arg1	heterogeneity					1598:1610	significantly higher structural heterogeneity	1566:1610	significantly higher structural heterogeneity	1566:1610	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	1	73	theme	therapeutic	242:252	arg1	mAb					277:279	mAb	277:279	mAb	277:279	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	1	73	theme	therapeutic	242:252	arg1	antibodies					265:274	therapeutic monoclonal antibodies	242:274	therapeutic monoclonal antibodies (mAb)	242:280	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	5	74	theme	Fab	974:976	arg1	region					978:983	the Fab region	970:983	the Fab region of full-size adalimumab	970:1007	We recently implemented this approach in the Fab region of full-size adalimumab and demonstrated that the thermodynamic stability of the Fab domain increases upon N-glycosite addition.					
34834161	11	75	theme	"	2294:2294	arg1	antibodies					2296:2305	"biobetter" antibodies	2284:2305	"biobetter" antibodies	2284:2305	Our findings shed light into mAb glycobiology and potential implications in the application of this technique for the development of "biobetter" antibodies.					
34834161	7	76	theme	mutants	1496:1502	arg1	profile					1481:1487	a detailed N-glycosylation profile	1454:1487	a detailed N-glycosylation profile of the mutants	1454:1502	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	4	77	theme	proximal	761:768	arg1	acids					755:759	amino acids	749:759	amino acids proximal to an aggregation-prone region	749:799	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	10	78	theme	additional	1912:1921	arg1	glycosylation					1923:1935	additional glycosylation	1912:1935	additional glycosylation	1912:1935	Further, we have studied effects on additional glycosylation on protein stability via accelerated studies by following protein folding and aggregation propensities and observed that additional glycosylation indeed enhances physical stability and prevent protein aggregation.					
34834161	11	79	theme	mAb	2180:2182	arg1	glycobiology					2184:2195	mAb glycobiology	2180:2195	mAb glycobiology	2180:2195	Our findings shed light into mAb glycobiology and potential implications in the application of this technique for the development of "biobetter" antibodies.					
34834161	7	80	from	positions	1340:1348	arg1	domain					1361:1366	the Fab domain	1353:1366	the Fab domain of Trastuzumab	1353:1381	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	7	80	from	positions	1340:1348	arg1	Trastuzumab					1371:1381	Trastuzumab	1371:1381	Trastuzumab	1371:1381	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	6	81	theme	occupancy	1216:1224	arg1	confirmation					1193:1204	appropriate confirmation	1181:1204	appropriate confirmation of glycan occupancy	1181:1224	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	6	81	theme	occupancy	1216:1224	arg1	characterization					1241:1256	structural characterization	1230:1256	structural characterization of the ensuing glycan profile	1230:1286	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	0	82	theme	Glycosylation	72:84	arg1	Sequons					86:92	Rationally Added Glycosylation Sequons	55:92	Rationally Added Glycosylation Sequons	55:92	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity.					
34834161	10	83	from	effects	1901:1907	arg1	glycosylation					1923:1935	additional glycosylation	1912:1935	additional glycosylation	1912:1935	Further, we have studied effects on additional glycosylation on protein stability via accelerated studies by following protein folding and aggregation propensities and observed that additional glycosylation indeed enhances physical stability and prevent protein aggregation.					
34834161	10	83	from	effects	1901:1907	arg1	stability					1948:1956	protein stability	1940:1956	protein stability	1940:1956	Further, we have studied effects on additional glycosylation on protein stability via accelerated studies by following protein folding and aggregation propensities and observed that additional glycosylation indeed enhances physical stability and prevent protein aggregation.					
34834161	7	84	theme	N-glycosylation	1465:1479	arg1	profile					1481:1487	a detailed N-glycosylation profile	1454:1487	a detailed N-glycosylation profile of the mutants	1454:1502	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	4	85	theme	sequons	738:744	arg1	insertion					709:717	the insertion	705:717	the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region	705:799	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	7	86	theme	identified	1319:1328	arg1	positions					1340:1348	previously identified candidate positions	1308:1348	previously identified candidate positions on the Fab domain of Trastuzumab	1308:1381	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	8	87	gly	N-glycosylation	1678:1692	arg2	site					1694:1697	a single N-glycosylation site	1669:1697	a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region	1669:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	4	88	theme	amino	749:753	arg1	acids					755:759	amino acids	749:759	amino acids proximal to an aggregation-prone region	749:799	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	0	89	theme	Increased	117:125	arg1	Complexity					134:143	Significantly Increased Glycan Complexity	103:143	Significantly Increased Glycan Complexity	103:143	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity.					
34834161	4	90	theme	protein	844:850	arg1	stability					827:835	the physical stability	814:835	the physical stability of the protein	814:850	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	2	91	theme	production	334:343	arg1	yield					345:349	production yield	334:349	production yield	334:349	Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity of the antibody monomer to bind to its cognate antigen.					
34834161	8	92	theme	single	1671:1676	arg1	site					1694:1697	a single N-glycosylation site	1669:1697	a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region	1669:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	4	93	theme	physical	818:825	arg1	stability					827:835	the physical stability	814:835	the physical stability of the protein	814:850	It was shown that the insertion of N-glycosylation sequons on amino acids proximal to an aggregation-prone region can increase the physical stability of the protein by shielding the APR, thus preventing self-association of antibody monomers.					
34834161	0	94	theme	Profile	7:13	arg1	Analysis					15:22	Glycan Profile Analysis	0:22	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons	0:92	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity.					
34834161	5	95	theme	adalimumab	998:1007	arg1	region					978:983	the Fab region	970:983	the Fab region of full-size adalimumab	970:1007	We recently implemented this approach in the Fab region of full-size adalimumab and demonstrated that the thermodynamic stability of the Fab domain increases upon N-glycosite addition.					
34834161	11	96	theme	technique	2251:2259	arg1	application					2231:2241	the application	2227:2241	the application of this technique	2227:2259	Our findings shed light into mAb glycobiology and potential implications in the application of this technique for the development of "biobetter" antibodies.					
34834161	8	97	theme	heavy	1703:1707	arg1	chain					1709:1713	heavy chain	1703:1713	heavy chain	1703:1713	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	2	98	theme	pharmaceutical	412:425	arg1	product					427:433	the pharmaceutical product	408:433	the pharmaceutical product	408:433	Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity of the antibody monomer to bind to its cognate antigen.					
34834161	2	99	theme	antibody	466:473	arg1	monomer					475:481	the antibody monomer	462:481	the antibody monomer	462:481	Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity of the antibody monomer to bind to its cognate antigen.					
34834161	8	100	theme	located	1715:1721	arg1	site					1694:1697	a single N-glycosylation site	1669:1697	a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region	1669:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	0	101	theme	Trastuzumab	38:48	arg1	Analysis					15:22	Glycan Profile Analysis	0:22	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons	0:92	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity.					
34834161	7	102	theme	mass	1403:1406	arg1	spectrometry					1408:1419	tandem mass spectrometry	1396:1419	tandem mass spectrometry	1396:1419	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	2	103	theme	cognate	498:504	arg1	antigen					506:512	its cognate antigen	494:512	its cognate antigen	494:512	Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity of the antibody monomer to bind to its cognate antigen.					
34834161	8	104	theme	Trastuzumab	1509:1519	arg1	glycomutants					1521:1532	The Trastuzumab glycomutants	1505:1532	The Trastuzumab glycomutants	1505:1532	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	6	105	theme	structural	1230:1239	arg1	characterization					1241:1256	structural characterization	1230:1256	structural characterization of the ensuing glycan profile	1230:1286	Previous experimental data reported for this technique have lacked appropriate confirmation of glycan occupancy and structural characterization of the ensuing glycan profile.					
34834161	1	106	dep	manufacture	210:220	arg1	the					206:208	the	206:208	the	206:208	Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives.					
34834161	7	107	theme	Trastuzumab	1371:1381	arg1	domain					1361:1366	the Fab domain	1353:1366	the Fab domain of Trastuzumab	1353:1381	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	7	107	theme	Trastuzumab	1371:1381	arg1	Trastuzumab					1371:1381	Trastuzumab	1371:1381	Trastuzumab	1371:1381	Herein, we mutated previously identified candidate positions on the Fab domain of Trastuzumab and employed tandem mass spectrometry to confirm attachment and obtain a detailed N-glycosylation profile of the mutants.					
34834161	8	108	theme	glycan	1546:1551	arg1	profile					1553:1559	a glycan profile	1544:1559	a glycan profile with significantly higher structural heterogeneity	1544:1610	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	2	109	theme	immunogenic	358:368	arg1	reactions					370:378	immunogenic reactions	358:378	immunogenic reactions	358:378	Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity of the antibody monomer to bind to its cognate antigen.					
34834161	5	110	theme	domain	1070:1075	arg1	stability					1049:1057	the thermodynamic stability	1031:1057	the thermodynamic stability of the Fab domain	1031:1075	We recently implemented this approach in the Fab region of full-size adalimumab and demonstrated that the thermodynamic stability of the Fab domain increases upon N-glycosite addition.					
34834161	8	111	theme	region	1751:1756	arg1	region					1751:1756	the Fc region	1744:1756	the Fc region	1744:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	8	111	theme	region	1751:1756	arg1	domain					1734:1739	the CH2 domain	1726:1739	the CH2 domain of the Fc region	1726:1756	The Trastuzumab glycomutants displayed a glycan profile with significantly higher structural heterogeneity compared to the HEK Trastuzumab antibody, which contains a single N-glycosylation site per heavy chain located in the CH2 domain of the Fc region.					
34834161	0	112	with	Trastuzumab	38:48	arg1	Sequons					86:92	Rationally Added Glycosylation Sequons	55:92	Rationally Added Glycosylation Sequons	55:92	Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity.					
33076454	0	0	theme	Glycosylation	95:107	arg1	Disorders					82:90	Congenital Disorders	71:90	Congenital Disorders of Glycosylation	71:107	Dissecting Total Plasma and Protein-Specific Glycosylation Profiles in Congenital Disorders of Glycosylation.					
33076454	0	1	from	Plasma	17:22	arg1	Disorders					82:90	Congenital Disorders	71:90	Congenital Disorders of Glycosylation	71:107	Dissecting Total Plasma and Protein-Specific Glycosylation Profiles in Congenital Disorders of Glycosylation.					
33076454	4	2	from	patients	642:649	arg1	N-glycans					557:565	enzymatically released N-glycans	534:565	enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls	534:670	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	7	3	theme	unprecedented	1041:1053	arg1	effects					1079:1085	unprecedented sialic linkage-specific effects	1041:1085	unprecedented sialic linkage-specific effects	1041:1085	Next to the previously described glycomic effects, we report unprecedented sialic linkage-specific effects.					
33076454	1	4	theme	biological	179:188	arg1	processes					190:198	many biological processes	174:198	many biological processes	174:198	Protein N-glycosylation is a multifactorial process involved in many biological processes.					
33076454	3	5	from	defects	434:440	arg1	transport					449:457	the transport	445:457	the transport of nucleotide sugars	445:478	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	3	5	from	defects	434:440	arg1	glycosylation					487:499	Golgi glycosylation	481:499	Golgi glycosylation	481:499	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	3	5	from	defects	434:440	arg1	trafficking					510:520	Golgi trafficking	504:520	Golgi trafficking	504:520	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	6	6	theme	IgG	888:890	arg1	Fc					892:893	IgG Fc	888:893	IgG Fc	888:893	Furthermore, protein-specific glycan profiles were quantified for transferrin and IgG Fc using electrospray ionization MS of intact proteins and glycopeptides, respectively.					
33076454	10	7	theme	broad	1492:1496	arg1	range					1498:1502	the broad range	1488:1502	the broad range of biological mechanisms that influence protein glycosylation	1488:1564	The diverse abnormalities in glycomic features described in this study reflect the broad range of biological mechanisms that influence protein glycosylation.					
33076454	8	8	gly	sialylated	1221:1230	arg1	structures					1232:1241	sialylated structures	1221:1241	sialylated structures on plasma proteins	1221:1260	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	4	9	theme	G	629:629	arg1	N-glycans					557:565	enzymatically released N-glycans	534:565	enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls	534:670	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	3	10	theme	disrupted	373:381	arg1	N-glycosylation					383:397	the disrupted N-glycosylation	369:397	the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking	369:520	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	4	11	theme	released	548:555	arg1	N-glycans					557:565	enzymatically released N-glycans	534:565	enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls	534:670	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	8	12	theme	Golgi	1120:1124	arg1	COG5-CDG					1139:1146	COG5-CDG	1139:1146	COG5-CDG	1139:1146	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	8	12	theme	Golgi	1120:1124	arg1	trafficking					1126:1136	Golgi trafficking	1120:1136	Golgi trafficking (COG5-CDG)	1120:1147	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	2	13	theme	protein	307:313	arg1	biosynthesis					324:335	protein N-glycan biosynthesis	307:335	protein N-glycan biosynthesis	307:335	A broad range of congenital disorders of glycosylation (CDGs) have been described that feature defects in protein N-glycan biosynthesis.					
33076454	5	14	theme	linkage	764:770	arg1	isomers					772:778	sialic acid linkage isomers	752:778	sialic acid linkage isomers	752:778	The applied method allowed the differentiation of sialic acid linkage isomers via their derivatization.					
33076454	6	15	theme	glycopeptides	951:963	arg1	MS					925:926	electrospray ionization MS	901:926	electrospray ionization MS of intact proteins and glycopeptides	901:963	Furthermore, protein-specific glycan profiles were quantified for transferrin and IgG Fc using electrospray ionization MS of intact proteins and glycopeptides, respectively.					
33076454	7	16	theme	glycomic	1013:1020	arg1	effects					1022:1028	the previously described glycomic effects	988:1028	the previously described glycomic effects	988:1028	Next to the previously described glycomic effects, we report unprecedented sialic linkage-specific effects.					
33076454	5	17	theme	isomers	772:778	arg1	differentiation					733:747	the differentiation	729:747	the differentiation of sialic acid linkage isomers	729:778	The applied method allowed the differentiation of sialic acid linkage isomers via their derivatization.					
33076454	0	18	from	Profiles	59:66	arg1	Disorders					82:90	Congenital Disorders	71:90	Congenital Disorders of Glycosylation	71:107	Dissecting Total Plasma and Protein-Specific Glycosylation Profiles in Congenital Disorders of Glycosylation.					
33076454	8	19	theme	plasma	1246:1251	arg1	proteins					1253:1260	plasma proteins	1246:1260	plasma proteins	1246:1260	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	8	20	theme	CMP-sialic	1153:1162	arg1	acid					1164:1167	CMP-sialic acid	1153:1167	CMP-sialic acid transport (SLC35A1-CDG)	1153:1191	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	10	21	theme	protein	1544:1550	arg1	glycosylation					1552:1564	protein glycosylation	1544:1564	protein glycosylation	1544:1564	The diverse abnormalities in glycomic features described in this study reflect the broad range of biological mechanisms that influence protein glycosylation.					
33076454	3	22	theme	nucleotide	462:471	arg1	sugars					473:478	nucleotide sugars	462:478	nucleotide sugars	462:478	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	6	23	theme	proteins	938:945	arg1	MS					925:926	electrospray ionization MS	901:926	electrospray ionization MS of intact proteins and glycopeptides	901:963	Furthermore, protein-specific glycan profiles were quantified for transferrin and IgG Fc using electrospray ionization MS of intact proteins and glycopeptides, respectively.					
33076454	10	24	theme	mechanisms	1518:1527	arg1	range					1498:1502	the broad range	1488:1502	the broad range of biological mechanisms that influence protein glycosylation	1488:1564	The diverse abnormalities in glycomic features described in this study reflect the broad range of biological mechanisms that influence protein glycosylation.					
33076454	9	25	theme	specific	1314:1321	arg1	CDGs					1323:1326	these specific CDGs	1308:1326	these specific CDGs	1308:1326	Findings for these specific CDGs include a more pronounced effect for α2,3-sialylation than for α2,6-sialylation.					
33076454	3	26	theme	sugars	473:478	arg1	transport					449:457	the transport	445:457	the transport of nucleotide sugars	445:478	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	3	26	theme	sugars	473:478	arg1	glycosylation					487:499	Golgi glycosylation	481:499	Golgi glycosylation	481:499	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	3	26	theme	sugars	473:478	arg1	trafficking					510:520	Golgi trafficking	504:520	Golgi trafficking	504:520	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	0	27	theme	Total	11:15	arg1	Plasma					17:22	Total Plasma and Protein-Specific Glycosylation Profiles	11:66	Plasma	17:22	Dissecting Total Plasma and Protein-Specific Glycosylation Profiles in Congenital Disorders of Glycosylation.					
33076454	6	28	theme	intact	931:936	arg1	proteins					938:945	intact proteins	931:945	intact proteins	931:945	Furthermore, protein-specific glycan profiles were quantified for transferrin and IgG Fc using electrospray ionization MS of intact proteins and glycopeptides, respectively.					
33076454	6	29	theme	glycan	836:841	arg1	profiles					843:850	protein-specific glycan profiles	819:850	protein-specific glycan profiles	819:850	Furthermore, protein-specific glycan profiles were quantified for transferrin and IgG Fc using electrospray ionization MS of intact proteins and glycopeptides, respectively.					
33076454	4	30	dep	purified	605:612	arg1	affinity					596:603	affinity	596:603	affinity	596:603	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	3	31	theme	patients	414:421	arg1	N-glycosylation					383:397	the disrupted N-glycosylation	369:397	the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking	369:520	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	9	32	theme	pronounced	1343:1352	arg1	effect					1354:1359	a more pronounced effect	1336:1359	a more pronounced effect for α2,3-sialylation than for α2,6-sialylation	1336:1406	Findings for these specific CDGs include a more pronounced effect for α2,3-sialylation than for α2,6-sialylation.					
33076454	6	33	theme	protein-specific	819:834	arg1	profiles					843:850	protein-specific glycan profiles	819:850	protein-specific glycan profiles	819:850	Furthermore, protein-specific glycan profiles were quantified for transferrin and IgG Fc using electrospray ionization MS of intact proteins and glycopeptides, respectively.					
33076454	1	34	theme	Protein	110:116	arg1	process					154:160	a multifactorial process	137:160	a multifactorial process involved in many biological processes	137:198	Protein N-glycosylation is a multifactorial process involved in many biological processes.					
33076454	1	34	theme	Protein	110:116	arg1	N-glycosylation					118:132	Protein N-glycosylation	110:132	Protein N-glycosylation	110:132	Protein N-glycosylation is a multifactorial process involved in many biological processes.					
33076454	8	35	from	levels	1211:1216	arg1	proteins					1253:1260	plasma proteins	1246:1260	plasma proteins	1246:1260	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	3	36	gly	N-glycosylation	383:397	arg1	patients					414:421	various CDG patients	402:421	various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking	402:520	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	3	37	theme	various	402:408	arg1	patients					414:421	various CDG patients	402:421	various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking	402:520	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	4	38	theme	immunoglobulin	614:627	arg1	IgG					632:634	IgG	632:634	IgG	632:634	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	4	38	theme	immunoglobulin	614:627	arg1	G					629:629	affinity purified immunoglobulin G	596:629	affinity purified immunoglobulin G (IgG)	596:635	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	8	39	from	proteins	1253:1260	arg1	levels					1211:1216	lower levels	1205:1216	lower levels of sialylated structures on plasma proteins	1205:1260	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	2	40	theme	N-glycan	315:322	arg1	biosynthesis					324:335	protein N-glycan biosynthesis	307:335	protein N-glycan biosynthesis	307:335	A broad range of congenital disorders of glycosylation (CDGs) have been described that feature defects in protein N-glycan biosynthesis.					
33076454	10	41	theme	biological	1507:1516	arg1	mechanisms					1518:1527	biological mechanisms	1507:1527	biological mechanisms that influence protein glycosylation	1507:1564	The diverse abnormalities in glycomic features described in this study reflect the broad range of biological mechanisms that influence protein glycosylation.					
33076454	0	42	theme	Glycosylation	45:57	arg1	Profiles					59:66	Total Plasma and Protein-Specific Glycosylation Profiles	11:66	Profiles	59:66	Dissecting Total Plasma and Protein-Specific Glycosylation Profiles in Congenital Disorders of Glycosylation.					
33076454	4	43	theme	purified	605:612	arg1	IgG					632:634	IgG	632:634	IgG	632:634	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	4	43	theme	purified	605:612	arg1	G					629:629	affinity purified immunoglobulin G	596:629	affinity purified immunoglobulin G (IgG)	596:635	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	2	44	theme	broad	203:207	arg1	range					209:213	A broad range	201:213	A broad range of congenital disorders of glycosylation (CDGs)	201:261	A broad range of congenital disorders of glycosylation (CDGs) have been described that feature defects in protein N-glycan biosynthesis.					
33076454	4	45	from	controls	663:670	arg1	N-glycans					557:565	enzymatically released N-glycans	534:565	enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls	534:670	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	0	46	theme	Protein-Specific	28:43	arg1	Profiles					59:66	Total Plasma and Protein-Specific Glycosylation Profiles	11:66	Profiles	59:66	Dissecting Total Plasma and Protein-Specific Glycosylation Profiles in Congenital Disorders of Glycosylation.					
33076454	4	47	theme	total	570:574	arg1	proteins					583:590	total plasma proteins	570:590	total plasma proteins	570:590	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	5	48	theme	sialic	752:757	arg1	isomers					772:778	sialic acid linkage isomers	752:778	sialic acid linkage isomers	752:778	The applied method allowed the differentiation of sialic acid linkage isomers via their derivatization.					
33076454	6	49	theme	ionization	914:923	arg1	MS					925:926	electrospray ionization MS	901:926	electrospray ionization MS of intact proteins and glycopeptides	901:963	Furthermore, protein-specific glycan profiles were quantified for transferrin and IgG Fc using electrospray ionization MS of intact proteins and glycopeptides, respectively.					
33076454	3	50	gly	glycosylation	487:499	arg1	sugars					473:478	nucleotide sugars	462:478	nucleotide sugars	462:478	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	10	51	theme	glycomic	1438:1445	arg1	features					1447:1454	glycomic features	1438:1454	glycomic features described in this study	1438:1478	The diverse abnormalities in glycomic features described in this study reflect the broad range of biological mechanisms that influence protein glycosylation.					
33076454	3	52	theme	Golgi	481:485	arg1	glycosylation					487:499	Golgi glycosylation	481:499	Golgi glycosylation	481:499	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	5	53	theme	acid	759:762	arg1	isomers					772:778	sialic acid linkage isomers	752:778	sialic acid linkage isomers	752:778	The applied method allowed the differentiation of sialic acid linkage isomers via their derivatization.					
33076454	8	54	theme	healthy	1277:1283	arg1	controls					1285:1292	healthy controls	1277:1292	healthy controls	1277:1292	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	6	55	theme	electrospray	901:912	arg1	MS					925:926	electrospray ionization MS	901:926	electrospray ionization MS of intact proteins and glycopeptides	901:963	Furthermore, protein-specific glycan profiles were quantified for transferrin and IgG Fc using electrospray ionization MS of intact proteins and glycopeptides, respectively.					
33076454	8	56	theme	acid	1164:1167	arg1	SLC35A1-CDG					1180:1190	SLC35A1-CDG	1180:1190	SLC35A1-CDG	1180:1190	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	8	56	theme	acid	1164:1167	arg1	transport					1169:1177	CMP-sialic acid transport	1153:1177	CMP-sialic acid transport (SLC35A1-CDG)	1153:1191	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	4	57	theme	proteins	583:590	arg1	N-glycans					557:565	enzymatically released N-glycans	534:565	enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls	534:670	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	5	58	theme	applied	706:712	arg1	method					714:719	The applied method	702:719	The applied method	702:719	The applied method allowed the differentiation of sialic acid linkage isomers via their derivatization.					
33076454	2	59	theme	glycosylation	242:254	arg1	CDGs					257:260	CDGs	257:260	CDGs	257:260	A broad range of congenital disorders of glycosylation (CDGs) have been described that feature defects in protein N-glycan biosynthesis.					
33076454	2	59	theme	glycosylation	242:254	arg1	disorders					229:237	congenital disorders	218:237	congenital disorders of glycosylation (CDGs)	218:261	A broad range of congenital disorders of glycosylation (CDGs) have been described that feature defects in protein N-glycan biosynthesis.					
33076454	1	60	theme	multifactorial	139:152	arg1	process					154:160	a multifactorial process	137:160	a multifactorial process involved in many biological processes	137:198	Protein N-glycosylation is a multifactorial process involved in many biological processes.					
33076454	1	60	theme	multifactorial	139:152	arg1	N-glycosylation					118:132	Protein N-glycosylation	110:132	Protein N-glycosylation	110:132	Protein N-glycosylation is a multifactorial process involved in many biological processes.					
33076454	4	61	theme	plasma	576:581	arg1	proteins					583:590	total plasma proteins	570:590	total plasma proteins	570:590	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	2	62	theme	disorders	229:237	arg1	range					209:213	A broad range	201:213	A broad range of congenital disorders of glycosylation (CDGs)	201:261	A broad range of congenital disorders of glycosylation (CDGs) have been described that feature defects in protein N-glycan biosynthesis.					
33076454	8	63	from	structures	1232:1241	arg1	proteins					1253:1260	plasma proteins	1246:1260	plasma proteins	1246:1260	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	10	64	theme	diverse	1413:1419	arg1	abnormalities					1421:1433	The diverse abnormalities	1409:1433	The diverse abnormalities in glycomic features described in this study	1409:1478	The diverse abnormalities in glycomic features described in this study reflect the broad range of biological mechanisms that influence protein glycosylation.					
33076454	3	65	theme	Golgi	504:508	arg1	trafficking					510:520	Golgi trafficking	504:520	Golgi trafficking	504:520	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	6	66	gly	glycopeptides	951:963	arg2	glycopeptides					951:963	glycopeptides	951:963	glycopeptides	951:963	Furthermore, protein-specific glycan profiles were quantified for transferrin and IgG Fc using electrospray ionization MS of intact proteins and glycopeptides, respectively.					
33076454	8	67	theme	structures	1232:1241	arg1	levels					1211:1216	lower levels	1205:1216	lower levels of sialylated structures on plasma proteins	1205:1260	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	2	68	theme	congenital	218:227	arg1	CDGs					257:260	CDGs	257:260	CDGs	257:260	A broad range of congenital disorders of glycosylation (CDGs) have been described that feature defects in protein N-glycan biosynthesis.					
33076454	2	68	theme	congenital	218:227	arg1	disorders					229:237	congenital disorders	218:237	congenital disorders of glycosylation (CDGs)	218:261	A broad range of congenital disorders of glycosylation (CDGs) have been described that feature defects in protein N-glycan biosynthesis.					
33076454	7	69	theme	sialic	1055:1060	arg1	effects					1079:1085	unprecedented sialic linkage-specific effects	1041:1085	unprecedented sialic linkage-specific effects	1041:1085	Next to the previously described glycomic effects, we report unprecedented sialic linkage-specific effects.					
33076454	7	70	theme	described	1003:1011	arg1	effects					1022:1028	the previously described glycomic effects	988:1028	the previously described glycomic effects	988:1028	Next to the previously described glycomic effects, we report unprecedented sialic linkage-specific effects.					
33076454	10	71	from	abnormalities	1421:1433	arg1	features					1447:1454	glycomic features	1438:1454	glycomic features described in this study	1438:1478	The diverse abnormalities in glycomic features described in this study reflect the broad range of biological mechanisms that influence protein glycosylation.					
33076454	8	72	theme	lower	1205:1209	arg1	levels					1211:1216	lower levels	1205:1216	lower levels of sialylated structures on plasma proteins	1205:1260	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	4	73	theme	mass	678:681	arg1	MS					697:698	MS	697:698	MS	697:698	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	4	73	theme	mass	678:681	arg1	spectrometry					683:694	mass spectrometry	678:694	mass spectrometry (MS)	678:699	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	7	74	theme	linkage-specific	1062:1077	arg1	effects					1079:1085	unprecedented sialic linkage-specific effects	1041:1085	unprecedented sialic linkage-specific effects	1041:1085	Next to the previously described glycomic effects, we report unprecedented sialic linkage-specific effects.					
33076454	8	75	from	Defects	1088:1094	arg1	proteins					1099:1106	proteins	1099:1106	proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG)	1099:1191	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
33076454	3	76	theme	CDG	410:412	arg1	patients					414:421	various CDG patients	402:421	various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking	402:520	Here, we present insights into the disrupted N-glycosylation of various CDG patients exhibiting defects in the transport of nucleotide sugars, Golgi glycosylation or Golgi trafficking.					
33076454	4	77	theme	healthy	655:661	arg1	controls					663:670	healthy controls	655:670	healthy controls	655:670	We studied enzymatically released N-glycans of total plasma proteins and affinity purified immunoglobulin G (IgG) from patients and healthy controls using mass spectrometry (MS).					
33076454	0	78	theme	Congenital	71:80	arg1	Disorders					82:90	Congenital Disorders	71:90	Congenital Disorders of Glycosylation	71:107	Dissecting Total Plasma and Protein-Specific Glycosylation Profiles in Congenital Disorders of Glycosylation.					
33076454	1	79	theme	many	174:177	arg1	processes					190:198	many biological processes	174:198	many biological processes	174:198	Protein N-glycosylation is a multifactorial process involved in many biological processes.					
33076454	8	80	theme	sialylated	1221:1230	arg1	structures					1232:1241	sialylated structures	1221:1241	sialylated structures on plasma proteins	1221:1260	Defects in proteins involved in Golgi trafficking (COG5-CDG) and CMP-sialic acid transport (SLC35A1-CDG) resulted in lower levels of sialylated structures on plasma proteins as compared to healthy controls.					
32125558	2	0	theme	anti-inflammatory	331:347	arg1	efficacy					400:407	antibody-dependent cellular cytotoxicity efficacy	359:407	antibody-dependent cellular cytotoxicity efficacy of IgG antibodies	359:425	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	2	0	theme	anti-inflammatory	331:347	arg1	activity					349:356	anti-inflammatory activity	331:356	anti-inflammatory activity	331:356	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	1	1	theme	biopharmaceutical	114:130	arg1	products					132:139	commercial biopharmaceutical products	103:139	commercial biopharmaceutical products	103:139	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	8	2	theme	N-glycolylneuraminic	1432:1451	arg1	composition					1467:1477	N-glycolylneuraminic acid terminal composition	1432:1477	N-glycolylneuraminic acid terminal composition	1432:1477	Moreover, the resultant glycan structures mostly had N-acetylneuraminic acid terminals, while N-glycolylneuraminic acid terminal composition remained less than 5% as compared to the wild type.					
32125558	1	3	theme	hamster	171:177	arg1	cells					191:195	Chinese hamster ovary (CHO) cells	163:195	Chinese hamster ovary (CHO) cells	163:195	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	3	4	theme	acid-based	580:589	arg1	-sialyltransferase					610:627	α(2,6)-sialyltransferase	604:627	α(2,6)-sialyltransferase	604:627	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	4	theme	acid-based	580:589	arg1	kinase					689:694	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	633:694	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	633:694	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	4	theme	acid-based	580:589	arg1	enzymes					595:601	two N-acetylneuraminic acid-based key enzymes	557:601	two N-acetylneuraminic acid-based key enzymes	557:601	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	9	5	theme	Engineered	1531:1540	arg1	antibodies					1542:1551	Engineered antibodies	1531:1551	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid	1531:1619	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	1	6	theme	commercial	103:112	arg1	products					132:139	commercial biopharmaceutical products	103:139	commercial biopharmaceutical products	103:139	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	3	7	theme	2,6	606:608	arg1	-sialyltransferase					610:627	α(2,6)-sialyltransferase	604:627	α(2,6)-sialyltransferase	604:627	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	7	theme	2,6	606:608	arg1	enzymes					595:601	two N-acetylneuraminic acid-based key enzymes	557:601	two N-acetylneuraminic acid-based key enzymes	557:601	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	8	8	theme	wild	1520:1523	arg1	type					1525:1528	the wild type	1516:1528	the wild type	1516:1528	Moreover, the resultant glycan structures mostly had N-acetylneuraminic acid terminals, while N-glycolylneuraminic acid terminal composition remained less than 5% as compared to the wild type.					
32125558	3	9	theme	α	604:604	arg1	-sialyltransferase					610:627	α(2,6)-sialyltransferase	604:627	α(2,6)-sialyltransferase	604:627	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	9	theme	α	604:604	arg1	enzymes					595:601	two N-acetylneuraminic acid-based key enzymes	557:601	two N-acetylneuraminic acid-based key enzymes	557:601	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	4	10	from	type	889:892	arg1	extracts					911:918	total protein extracts	897:918	total protein extracts	897:918	Indeed, the number of total sialic acid terminal glycan structures increased tremendously, by 12-fold compared to the wild type in total protein extracts.					
32125558	9	11	theme	CHO	1566:1568	arg1	lines					1575:1579	CHO cell lines	1566:1579	CHO cell lines that produce high levels of sialic acid	1566:1619	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	4	12	theme	sialic	794:799	arg1	acid					801:804	total sialic acid	788:804	total sialic acid terminal glycan structures	788:831	Indeed, the number of total sialic acid terminal glycan structures increased tremendously, by 12-fold compared to the wild type in total protein extracts.					
32125558	8	13	theme	terminal	1458:1465	arg1	composition					1467:1477	N-glycolylneuraminic acid terminal composition	1432:1477	N-glycolylneuraminic acid terminal composition	1432:1477	Moreover, the resultant glycan structures mostly had N-acetylneuraminic acid terminals, while N-glycolylneuraminic acid terminal composition remained less than 5% as compared to the wild type.					
32125558	3	14	theme	UDP-N-acetylglucosamine	633:655	arg1	kinase					689:694	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	633:694	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	633:694	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	14	theme	UDP-N-acetylglucosamine	633:655	arg1	enzymes					595:601	two N-acetylneuraminic acid-based key enzymes	557:601	two N-acetylneuraminic acid-based key enzymes	557:601	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	1	15	theme	products	132:139	arg1	products					132:139	commercial biopharmaceutical products	103:139	commercial biopharmaceutical products	103:139	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	1	15	theme	products	132:139	arg1	%					98:98	about 70%	90:98	about 70% of commercial biopharmaceutical products	90:139	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	8	16	theme	resultant	1352:1360	arg1	structures					1369:1378	the resultant glycan structures	1348:1378	the resultant glycan structures	1348:1378	Moreover, the resultant glycan structures mostly had N-acetylneuraminic acid terminals, while N-glycolylneuraminic acid terminal composition remained less than 5% as compared to the wild type.					
32125558	5	17	gly	sialylated	1023:1032	arg1	structures					1034:1043	sialylated structures	1023:1043	sialylated structures of glycoforms in total proteins	1023:1075	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32125558	5	18	dep	1.4	1008:1010	arg1	to					1005:1006	to	1005:1006	to	1005:1006	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32125558	0	19	theme	hamster	63:69	arg1	cells					77:81	glyco-engineered Chinese hamster ovary cells	38:81	glyco-engineered Chinese hamster ovary cells	38:81	Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells.					
32125558	0	20	theme	sialylation	15:25	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells.	0:82	Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells.					
32125558	5	21	theme	targeted	962:969	arg1	CHOmt17-100					982:992	CHOmt17-100	982:992	CHOmt17-100	982:992	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32125558	5	21	theme	targeted	962:969	arg1	line					976:979	a targeted cell line	960:979	a targeted cell line	960:979	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32125558	0	22	from	Enhancement	0:10	arg1	rIgG					30:33	rIgG	30:33	rIgG in glyco-engineered Chinese hamster ovary cells	30:81	Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells.					
32125558	8	23	theme	acid	1410:1413	arg1	terminals					1415:1423	N-acetylneuraminic acid terminals	1391:1423	N-acetylneuraminic acid terminals	1391:1423	Moreover, the resultant glycan structures mostly had N-acetylneuraminic acid terminals, while N-glycolylneuraminic acid terminal composition remained less than 5% as compared to the wild type.					
32125558	4	24	theme	glycan	815:820	arg1	structures					822:831	total sialic acid terminal glycan structures	788:831	total sialic acid terminal glycan structures	788:831	Indeed, the number of total sialic acid terminal glycan structures increased tremendously, by 12-fold compared to the wild type in total protein extracts.					
32125558	5	25	theme	sialylated	1023:1032	arg1	structures					1034:1043	sialylated structures	1023:1043	sialylated structures of glycoforms in total proteins	1023:1075	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32125558	6	26	gly	sialylated	1170:1179	arg1	structures					1181:1190	about 53% sialylated structures	1160:1190	about 53% sialylated structures in its glycoforms	1160:1208	Interestingly, immunoglobulin G, used as the model protein in CHOmt17-100, showed about 53% sialylated structures in its glycoforms.					
32125558	0	27	from	rIgG	30:33	arg1	cells					77:81	glyco-engineered Chinese hamster ovary cells	38:81	glyco-engineered Chinese hamster ovary cells	38:81	Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells.					
32125558	9	28	attach	derived	1553:1559	arg1	lines					1575:1579	CHO cell lines	1566:1579	CHO cell lines that produce high levels of sialic acid	1566:1619	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	9	28	attach	derived	1553:1559	arg2	antibodies					1542:1551	Engineered antibodies	1531:1551	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid	1531:1619	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	4	29	theme	terminal	806:813	arg1	structures					822:831	total sialic acid terminal glycan structures	788:831	total sialic acid terminal glycan structures	788:831	Indeed, the number of total sialic acid terminal glycan structures increased tremendously, by 12-fold compared to the wild type in total protein extracts.					
32125558	2	30	theme	cytotoxicity	387:398	arg1	efficacy					400:407	antibody-dependent cellular cytotoxicity efficacy	359:407	antibody-dependent cellular cytotoxicity efficacy of IgG antibodies	359:425	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	2	30	theme	cytotoxicity	387:398	arg1	activity					349:356	anti-inflammatory activity	331:356	anti-inflammatory activity	331:356	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	1	31	theme	biopharmaceuticals	243:260	arg1	production					262:271	biopharmaceuticals production	243:271	biopharmaceuticals production	243:271	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	4	32	theme	structures	822:831	arg1	number					778:783	the number	774:783	the number of total sialic acid terminal glycan structures	774:831	Indeed, the number of total sialic acid terminal glycan structures increased tremendously, by 12-fold compared to the wild type in total protein extracts.					
32125558	7	33	theme	resultant	1217:1225	arg1	glycans					1238:1244	These resultant sialylated glycans	1211:1244	These resultant sialylated glycans	1211:1244	These resultant sialylated glycans exhibited more than approximately 14.5 times increase as compared to that of the wild type.					
32125558	3	34	theme	dihydrofolate	702:714	arg1	method					758:763	dihydrofolate reductase/methotrexate gene amplification method	702:763	dihydrofolate reductase/methotrexate gene amplification method	702:763	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	2	35	theme	antibodies	416:425	arg1	efficacy					400:407	antibody-dependent cellular cytotoxicity efficacy	359:407	antibody-dependent cellular cytotoxicity efficacy of IgG antibodies	359:425	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	2	35	theme	antibodies	416:425	arg1	activity					349:356	anti-inflammatory activity	331:356	anti-inflammatory activity	331:356	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	9	36	theme	glycoforms	1659:1668	arg1	examination					1644:1654	the examination	1640:1654	the examination of glycoforms' efficacy and usefulness toward bio-better products	1640:1720	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	7	37	dep	exhibited	1246:1254	arg1	increase					1291:1298	increase	1291:1298	exhibited more than approximately 14.5 times increase as compared to that of the wild type	1246:1335	These resultant sialylated glycans exhibited more than approximately 14.5 times increase as compared to that of the wild type.					
32125558	4	38	theme	total	897:901	arg1	extracts					911:918	total protein extracts	897:918	total protein extracts	897:918	Indeed, the number of total sialic acid terminal glycan structures increased tremendously, by 12-fold compared to the wild type in total protein extracts.					
32125558	0	39	theme	glyco-engineered	38:53	arg1	cells					77:81	glyco-engineered Chinese hamster ovary cells	38:81	glyco-engineered Chinese hamster ovary cells	38:81	Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells.					
32125558	9	40	theme	efficacy	1671:1678	arg1	examination					1644:1654	the examination	1640:1654	the examination of glycoforms' efficacy and usefulness toward bio-better products	1640:1720	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	6	41	theme	sialylated	1170:1179	arg1	structures					1181:1190	about 53% sialylated structures	1160:1190	about 53% sialylated structures in its glycoforms	1160:1208	Interestingly, immunoglobulin G, used as the model protein in CHOmt17-100, showed about 53% sialylated structures in its glycoforms.					
32125558	3	42	theme	CHO	482:484	arg1	lines					491:495	CHO cell lines	482:495	CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method	482:763	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	9	43	theme	usefulness	1684:1693	arg1	examination					1644:1654	the examination	1640:1654	the examination of glycoforms' efficacy and usefulness toward bio-better products	1640:1720	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	7	44	theme	wild	1327:1330	arg1	type					1332:1335	the wild type	1323:1335	the wild type	1323:1335	These resultant sialylated glycans exhibited more than approximately 14.5 times increase as compared to that of the wild type.					
32125558	3	45	theme	gene	739:742	arg1	method					758:763	dihydrofolate reductase/methotrexate gene amplification method	702:763	dihydrofolate reductase/methotrexate gene amplification method	702:763	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	46	theme	sialic	517:522	arg1	terminals					529:537	sialic acid terminals	517:537	sialic acid terminals	517:537	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	5	47	theme	methotrexate	930:941	arg1	supplementation					943:957	the methotrexate supplementation	926:957	the methotrexate supplementation	926:957	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32125558	9	48	theme	bio-better	1702:1711	arg1	products					1713:1720	bio-better products	1702:1720	bio-better products	1702:1720	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	2	49	theme	Meanwhile	274:282	arg1	terminals					297:305	Meanwhile, sialic acid terminals	274:305	Meanwhile, sialic acid terminals	274:305	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	9	50	theme	high	1594:1597	arg1	levels					1599:1604	high levels	1594:1604	high levels of sialic acid	1594:1619	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	6	51	from	protein	1129:1135	arg1	CHOmt17-100					1140:1150	CHOmt17-100	1140:1150	CHOmt17-100	1140:1150	Interestingly, immunoglobulin G, used as the model protein in CHOmt17-100, showed about 53% sialylated structures in its glycoforms.					
32125558	1	52	theme	Chinese	163:169	arg1	cells					191:195	Chinese hamster ovary (CHO) cells	163:195	Chinese hamster ovary (CHO) cells	163:195	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	4	53	theme	protein	903:909	arg1	extracts					911:918	total protein extracts	897:918	total protein extracts	897:918	Indeed, the number of total sialic acid terminal glycan structures increased tremendously, by 12-fold compared to the wild type in total protein extracts.					
32125558	3	54	theme	key	591:593	arg1	-sialyltransferase					610:627	α(2,6)-sialyltransferase	604:627	α(2,6)-sialyltransferase	604:627	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	54	theme	key	591:593	arg1	kinase					689:694	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	633:694	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	633:694	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	54	theme	key	591:593	arg1	enzymes					595:601	two N-acetylneuraminic acid-based key enzymes	557:601	two N-acetylneuraminic acid-based key enzymes	557:601	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	1	55	theme	ovary	179:183	arg1	cells					191:195	Chinese hamster ovary (CHO) cells	163:195	Chinese hamster ovary (CHO) cells	163:195	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	2	56	theme	sialic	285:290	arg1	Meanwhile					274:282	Meanwhile	274:282	Meanwhile	274:282	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	2	56	theme	sialic	285:290	arg1	acid					292:295	sialic acid	285:295	sialic acid	285:295	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	1	57	theme	CHO	186:188	arg1	cells					191:195	Chinese hamster ovary (CHO) cells	163:195	Chinese hamster ovary (CHO) cells	163:195	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	8	58	contain	had	1387:1389	arg1	structures					1369:1378	the resultant glycan structures	1348:1378	the resultant glycan structures	1348:1378	Moreover, the resultant glycan structures mostly had N-acetylneuraminic acid terminals, while N-glycolylneuraminic acid terminal composition remained less than 5% as compared to the wild type.					
32125558	8	58	contain	had	1387:1389	arg2	terminals					1415:1423	N-acetylneuraminic acid terminals	1391:1423	N-acetylneuraminic acid terminals	1391:1423	Moreover, the resultant glycan structures mostly had N-acetylneuraminic acid terminals, while N-glycolylneuraminic acid terminal composition remained less than 5% as compared to the wild type.					
32125558	9	59	theme	cell	1570:1573	arg1	lines					1575:1579	CHO cell lines	1566:1579	CHO cell lines that produce high levels of sialic acid	1566:1619	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	3	60	theme	N-acetylneuraminic	561:578	arg1	-sialyltransferase					610:627	α(2,6)-sialyltransferase	604:627	α(2,6)-sialyltransferase	604:627	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	60	theme	N-acetylneuraminic	561:578	arg1	kinase					689:694	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	633:694	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	633:694	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	60	theme	N-acetylneuraminic	561:578	arg1	enzymes					595:601	two N-acetylneuraminic acid-based key enzymes	557:601	two N-acetylneuraminic acid-based key enzymes	557:601	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	6	61	theme	immunoglobulin	1093:1106	arg1	G					1108:1108	immunoglobulin G	1093:1108	immunoglobulin G	1093:1108	Interestingly, immunoglobulin G, used as the model protein in CHOmt17-100, showed about 53% sialylated structures in its glycoforms.					
32125558	4	62	theme	total	788:792	arg1	acid					801:804	total sialic acid	788:804	total sialic acid terminal glycan structures	788:831	Indeed, the number of total sialic acid terminal glycan structures increased tremendously, by 12-fold compared to the wild type in total protein extracts.					
32125558	0	63	theme	ovary	71:75	arg1	cells					77:81	glyco-engineered Chinese hamster ovary cells	38:81	glyco-engineered Chinese hamster ovary cells	38:81	Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells.					
32125558	8	64	theme	glycan	1362:1367	arg1	structures					1369:1378	the resultant glycan structures	1348:1378	the resultant glycan structures	1348:1378	Moreover, the resultant glycan structures mostly had N-acetylneuraminic acid terminals, while N-glycolylneuraminic acid terminal composition remained less than 5% as compared to the wild type.					
32125558	5	65	theme	cell	971:974	arg1	CHOmt17-100					982:992	CHOmt17-100	982:992	CHOmt17-100	982:992	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32125558	5	65	theme	cell	971:974	arg1	line					976:979	a targeted cell line	960:979	a targeted cell line	960:979	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32125558	9	66	theme	sialic	1609:1614	arg1	acid					1616:1619	sialic acid	1609:1619	sialic acid	1609:1619	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	8	67	theme	N-acetylneuraminic	1391:1408	arg1	terminals					1415:1423	N-acetylneuraminic acid terminals	1391:1423	N-acetylneuraminic acid terminals	1391:1423	Moreover, the resultant glycan structures mostly had N-acetylneuraminic acid terminals, while N-glycolylneuraminic acid terminal composition remained less than 5% as compared to the wild type.					
32125558	9	68	theme	acid	1616:1619	arg1	levels					1599:1604	high levels	1594:1604	high levels of sialic acid	1594:1619	Engineered antibodies derived from CHO cell lines that produce high levels of sialic acid will contribute to the examination of glycoforms' efficacy and usefulness toward bio-better products.					
32125558	7	69	theme	sialylated	1227:1236	arg1	glycans					1238:1244	These resultant sialylated glycans	1211:1244	These resultant sialylated glycans	1211:1244	These resultant sialylated glycans exhibited more than approximately 14.5 times increase as compared to that of the wild type.					
32125558	6	70	theme	model	1123:1127	arg1	protein					1129:1135	the model protein	1119:1135	the model protein in CHOmt17-100	1119:1150	Interestingly, immunoglobulin G, used as the model protein in CHOmt17-100, showed about 53% sialylated structures in its glycoforms.					
32125558	4	71	theme	acid	801:804	arg1	structures					822:831	total sialic acid terminal glycan structures	788:831	total sialic acid terminal glycan structures	788:831	Indeed, the number of total sialic acid terminal glycan structures increased tremendously, by 12-fold compared to the wild type in total protein extracts.					
32125558	2	72	theme	cellular	378:385	arg1	cytotoxicity					387:398	antibody-dependent cellular cytotoxicity	359:398	antibody-dependent cellular cytotoxicity efficacy of IgG antibodies	359:425	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	8	73	theme	acid	1453:1456	arg1	composition					1467:1477	N-glycolylneuraminic acid terminal composition	1432:1477	N-glycolylneuraminic acid terminal composition	1432:1477	Moreover, the resultant glycan structures mostly had N-acetylneuraminic acid terminals, while N-glycolylneuraminic acid terminal composition remained less than 5% as compared to the wild type.					
32125558	2	74	theme	antibody-dependent	359:376	arg1	cytotoxicity					387:398	antibody-dependent cellular cytotoxicity	359:398	antibody-dependent cellular cytotoxicity efficacy of IgG antibodies	359:425	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	3	75	theme	2-epimerase/N-acetylmannosamine	657:687	arg1	kinase					689:694	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	633:694	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	633:694	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	3	75	theme	2-epimerase/N-acetylmannosamine	657:687	arg1	enzymes					595:601	two N-acetylneuraminic acid-based key enzymes	557:601	two N-acetylneuraminic acid-based key enzymes	557:601	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	4	76	theme	wild	884:887	arg1	type					889:892	the wild type	880:892	the wild type in total protein extracts	880:918	Indeed, the number of total sialic acid terminal glycan structures increased tremendously, by 12-fold compared to the wild type in total protein extracts.					
32125558	2	77	theme	IgG	412:414	arg1	antibodies					416:425	IgG antibodies	412:425	IgG antibodies	412:425	Meanwhile, sialic acid terminals were reported to affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity efficacy of IgG antibodies.					
32125558	0	78	theme	Chinese	55:61	arg1	cells					77:81	glyco-engineered Chinese hamster ovary cells	38:81	glyco-engineered Chinese hamster ovary cells	38:81	Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells.					
32125558	3	79	theme	amplification	744:756	arg1	method					758:763	dihydrofolate reductase/methotrexate gene amplification method	702:763	dihydrofolate reductase/methotrexate gene amplification method	702:763	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	6	80	from	structures	1181:1190	arg1	glycoforms					1199:1208	its glycoforms	1195:1208	its glycoforms	1195:1208	Interestingly, immunoglobulin G, used as the model protein in CHOmt17-100, showed about 53% sialylated structures in its glycoforms.					
32125558	5	81	theme	glycoforms	1048:1057	arg1	structures					1034:1043	sialylated structures	1023:1043	sialylated structures of glycoforms in total proteins	1023:1075	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32125558	3	82	theme	reductase/methotrexate	716:737	arg1	method					758:763	dihydrofolate reductase/methotrexate gene amplification method	702:763	dihydrofolate reductase/methotrexate gene amplification method	702:763	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	7	83	gly	sialylated	1227:1236	arg1	glycans					1238:1244	These resultant sialylated glycans	1211:1244	These resultant sialylated glycans	1211:1244	These resultant sialylated glycans exhibited more than approximately 14.5 times increase as compared to that of the wild type.					
32125558	3	84	theme	cell	486:489	arg1	lines					491:495	CHO cell lines	482:495	CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method	482:763	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	1	85	theme	cell	203:206	arg1	line					208:211	this cell line	198:211	this cell line	198:211	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	1	85	theme	cell	203:206	arg1	workhorse					229:237	a workhorse	227:237	a workhorse for biopharmaceuticals production	227:271	Since about 70% of commercial biopharmaceutical products have been produced in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for biopharmaceuticals production.					
32125558	5	86	theme	total	1062:1066	arg1	proteins					1068:1075	total proteins	1062:1075	total proteins	1062:1075	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32125558	6	87	dep	sialylated	1170:1179	arg1	%					1168:1168	about 53%	1160:1168	about 53%	1160:1168	Interestingly, immunoglobulin G, used as the model protein in CHOmt17-100, showed about 53% sialylated structures in its glycoforms.					
32125558	3	88	theme	acid	524:527	arg1	terminals					529:537	sialic acid terminals	517:537	sialic acid terminals	517:537	Taking these findings together, we aimed to establish CHO cell lines that highly produce sialic acid terminals by overexpressing two N-acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate reductase/methotrexate gene amplification method.					
32125558	5	89	from	structures	1034:1043	arg1	proteins					1068:1075	total proteins	1062:1075	total proteins	1062:1075	With the methotrexate supplementation, a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated structures of glycoforms in total proteins.					
32678978	6	0	theme	1:1	1717:1719	arg1	complexes					1721:1729	1:1 complexes	1717:1729	1:1 complexes with RBD	1717:1738	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site.					
32678978	0	1	theme	Interaction	148:158	arg1	Disruptor					120:128	a Disruptor	118:128	a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor	118:186	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	1	2	theme	coronavirus	240:250	arg1	emergence					193:201	emergence	193:201	emergence	193:201	The emergence and rapid proliferation of the novel coronavirus (SARS-CoV-2) resulted in a global pandemic, with over 6,000,000 cases and nearly 400,000 deaths reported worldwide by the end of May 2020.					
32678978	1	2	theme	coronavirus	240:250	arg1	proliferation					213:225	rapid proliferation	207:225	rapid proliferation	207:225	The emergence and rapid proliferation of the novel coronavirus (SARS-CoV-2) resulted in a global pandemic, with over 6,000,000 cases and nearly 400,000 deaths reported worldwide by the end of May 2020.					
32678978	7	3	theme	charge	1890:1895	arg1	patch					1868:1872	the contiguous patch	1853:1872	the contiguous patch of the positive charge on the RBD surface	1853:1914	This association alters the protein conformation (to maximize the contiguous patch of the positive charge on the RBD surface), resulting in a notable decrease in its ability to associate with ACE2.					
32678978	8	4	theme	RBD	2185:2187	arg1	surface					2189:2195	the RBD surface	2181:2195	the RBD surface	2181:2195	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	6	5	theme	site	1785:1788	arg1	presence					1756:1763	the presence	1752:1763	the presence of a single binding site	1752:1788	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site.					
32678978	0	6	theme	Cell	174:177	arg1	Receptor					179:186	Its Host Cell Receptor	165:186	Its Host Cell Receptor	165:186	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	9	7	theme	important	2223:2231	arg1	information					2245:2255	important mechanistic information	2223:2255	important mechanistic information on attenuation of the ACE2/RBD association by heparin	2223:2309	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	9	8	theme	medicines	2474:2482	arg1	repurposing					2446:2456	rational repurposing	2437:2456	rational repurposing of the existing medicines for treating COVID-19	2437:2504	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	6	9	theme	single	1770:1775	arg1	site					1785:1788	a single binding site	1768:1788	a single binding site	1768:1788	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site.					
32678978	5	10	with	interference	1563:1574	arg1	docking					1591:1597	the virus docking	1581:1597	the virus docking to the host cell receptor	1581:1623	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32678978	3	11	theme	choice	653:658	arg1	spectrometry					611:622	Native mass spectrometry	599:622	Native mass spectrometry (MS)	599:627	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	3	11	theme	choice	653:658	arg1	tool					645:648	a tool	643:648	a tool of choice	643:658	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	4	12	dep	targets	1062:1068	arg1	allows					1189:1194	allows	1189:1194	allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein	1189:1331	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	13	theme	SARS-CoV-2	861:870	arg1	element					910:916	a critical element	899:916	a critical element of the viral entry to the host cell	899:952	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	13	theme	SARS-CoV-2	861:870	arg1	S-protein					887:895	S-protein	887:895	S-protein	887:895	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	13	theme	SARS-CoV-2	861:870	arg1	protein					878:884	SARS-CoV-2 spike protein	861:884	SARS-CoV-2 spike protein (S-protein)	861:896	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	13	theme	SARS-CoV-2	861:870	arg1	site					977:980	its docking site	965:980	its docking site on the host cell surface	965:1005	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	0	14	from	Action	60:65	arg1	COVID-19					97:104	COVID-19	97:104	COVID-19	97:104	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	8	15	theme	longer	2063:2068	arg1	chains					2070:2075	the longer chains	2059:2075	the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface	2059:2195	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	4	16	theme	host	989:992	arg1	surface					999:1005	the host cell surface	985:1005	the host cell surface	985:1005	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	5	17	theme	critical	1493:1500	arg1	information					1502:1512	critical information	1493:1512	critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor	1493:1623	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32678978	4	18	theme	noncovalent	1241:1251	arg1	complexes					1253:1261	the noncovalent complexes	1237:1261	the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein	1237:1331	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	0	19	from	Mechanism	47:55	arg1	COVID-19					97:104	COVID-19	97:104	COVID-19	97:104	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	8	20	theme	due	2077:2079	arg1	chains					2070:2075	the longer chains	2059:2075	the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface	2059:2195	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	3	21	theme	mechanistic	678:688	arg1	studies					690:696	the mechanistic studies	674:696	the mechanistic studies of drug/therapeutic target interactions	674:736	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	9	22	theme	association	2288:2298	arg1	attenuation					2260:2270	attenuation	2260:2270	attenuation of the ACE2/RBD association by heparin	2260:2309	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	8	23	from	pronounced	2033:2042	arg1	case					2051:2054	the case	2047:2054	the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface	2047:2195	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	7	24	theme	notable	1933:1939	arg1	decrease					1941:1948	a notable decrease	1931:1948	a notable decrease in its ability to associate with ACE2	1931:1986	This association alters the protein conformation (to maximize the contiguous patch of the positive charge on the RBD surface), resulting in a notable decrease in its ability to associate with ACE2.					
32678978	3	25	theme	target	718:723	arg1	interactions					725:736	drug/therapeutic target interactions	701:736	drug/therapeutic target interactions	701:736	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	4	26	theme	critical	901:908	arg1	element					910:916	a critical element	899:916	a critical element of the viral entry to the host cell	899:952	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	26	theme	critical	901:908	arg1	protein					878:884	SARS-CoV-2 spike protein	861:884	SARS-CoV-2 spike protein (S-protein)	861:896	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	6	27	theme	short	1631:1635	arg1	oligomers					1702:1710	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers	1626:1710	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers	1626:1710	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site.					
32678978	0	28	theme	Action	60:65	arg1	Mechanism					47:55	the Mechanism	43:55	the Mechanism of Action of Repurposed Therapeutics in COVID-19	43:104	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	5	29	with	combination	1358:1368	arg1	modeling					1385:1392	molecular modeling	1375:1392	molecular modeling	1375:1392	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32678978	2	30	theme	therapeutics	459:470	arg1	range					441:445	a range	439:445	a range of existing therapeutics	439:470	A rush to find a cure prompted re-evaluation of a range of existing therapeutics vis-à-vis their potential role in treating COVID-19, placing a premium on analytical tools capable of supporting such efforts.					
32678978	4	31	theme	native	1108:1113	arg1	MS					1115:1116	native MS	1108:1116	native MS	1108:1116	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	0	32	theme	Repurposed	70:79	arg1	Therapeutics					81:92	Repurposed Therapeutics	70:92	Repurposed Therapeutics	70:92	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	7	33	theme	protein	1819:1825	arg1	conformation					1827:1838	the protein conformation	1815:1838	the protein conformation (to maximize the contiguous patch of the positive charge on the RBD surface)	1815:1915	This association alters the protein conformation (to maximize the contiguous patch of the positive charge on the RBD surface), resulting in a notable decrease in its ability to associate with ACE2.					
32678978	6	34	dep	short	1631:1635	arg1	pentasaccharide					1638:1652	pentasaccharide	1638:1652	pentasaccharide	1638:1652	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site.					
32678978	3	35	theme	high	816:819	arg1	level					821:825	a high level	814:825	a high level of structural heterogeneity	814:853	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	2	36	from	premium	535:541	arg1	tools					557:561	analytical tools	546:561	analytical tools capable of supporting such efforts	546:596	A rush to find a cure prompted re-evaluation of a range of existing therapeutics vis-à-vis their potential role in treating COVID-19, placing a premium on analytical tools capable of supporting such efforts.					
32678978	0	37	with	Interaction	148:158	arg1	Receptor					179:186	Its Host Cell Receptor	165:186	Its Host Cell Receptor	165:186	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	9	38	theme	native	2365:2370	arg1	MS					2372:2373	native MS	2365:2373	native MS coupled to gas-phase ion chemistry	2365:2408	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	4	39	theme	host	944:947	arg1	cell					949:952	the host cell	940:952	the host cell	940:952	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	3	40	theme	heterogeneity	841:853	arg1	level					821:825	a high level	814:825	a high level of structural heterogeneity	814:853	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	6	41	theme	long	1670:1673	arg1	oligomers					1702:1710	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers	1626:1710	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers	1626:1710	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site.					
32678978	5	42	theme	heparin	1418:1424	arg1	role					1410:1413	the role	1406:1413	the role of heparin in destabilizing the ACE2/RBD association to be studied	1406:1480	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32678978	5	43	theme	interference	1563:1574	arg1	mechanism					1546:1554	the molecular mechanism	1532:1554	the molecular mechanism of its interference with the virus docking to the host cell receptor	1532:1623	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32678978	0	44	theme	MS	22:23	arg1	Utility					4:10	The Utility	0:10	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.	0:187	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	4	45	gly	glycosylated	1024:1035	arg1	S-protein					887:895	S-protein	887:895	S-protein	887:895	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	45	gly	glycosylated	1024:1035	arg1	element					910:916	a critical element	899:916	a critical element of the viral entry to the host cell	899:952	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	45	gly	glycosylated	1024:1035	arg1	ACE2					959:962	ACE2	959:962	ACE2	959:962	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	45	gly	glycosylated	1024:1035	arg1	protein					878:884	SARS-CoV-2 spike protein	861:884	SARS-CoV-2 spike protein (S-protein)	861:896	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	45	gly	glycosylated	1024:1035	arg1	site					977:980	its docking site	965:980	its docking site on the host cell surface	965:1005	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	9	46	theme	gas-phase	2386:2394	arg1	chemistry					2400:2408	gas-phase ion chemistry	2386:2408	gas-phase ion chemistry	2386:2408	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	8	47	theme	ACE2	2131:2134	arg1	segments					2152:2159	the low-pI ACE2 and the heparin segments	2120:2159	the low-pI ACE2 and the heparin segments not accommodated on the RBD surface	2120:2195	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	4	48	theme	S-protein	1323:1331	arg1	ACE2					1273:1276	ACE2	1273:1276	ACE2	1273:1276	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	48	theme	S-protein	1323:1331	arg1	RBD					1311:1313	RBD	1311:1313	RBD	1311:1313	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	48	theme	S-protein	1323:1331	arg1	domain					1303:1308	the receptor-binding domain	1282:1308	the receptor-binding domain (RBD) of the S-protein	1282:1331	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	48	theme	S-protein	1323:1331	arg1	S-protein					1323:1331	the S-protein	1319:1331	the S-protein	1319:1331	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	49	theme	viral	925:929	arg1	entry					931:935	the viral entry	921:935	the viral entry to the host cell	921:952	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	50	theme	charge	1171:1176	arg1	technique					1152:1160	a gas-phase ion manipulation technique	1123:1160	a gas-phase ion manipulation technique (limited charge reduction)	1123:1187	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	50	theme	charge	1171:1176	arg1	reduction					1178:1186	limited charge reduction	1163:1186	limited charge reduction	1163:1186	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	2	51	theme	existing	450:457	arg1	therapeutics					459:470	existing therapeutics	450:470	existing therapeutics	450:470	A rush to find a cure prompted re-evaluation of a range of existing therapeutics vis-à-vis their potential role in treating COVID-19, placing a premium on analytical tools capable of supporting such efforts.					
32678978	1	52	theme	global	279:284	arg1	pandemic					286:293	a global pandemic	277:293	a global pandemic	277:293	The emergence and rapid proliferation of the novel coronavirus (SARS-CoV-2) resulted in a global pandemic, with over 6,000,000 cases and nearly 400,000 deaths reported worldwide by the end of May 2020.					
32678978	4	53	theme	gas-phase	1125:1133	arg1	technique					1152:1160	a gas-phase ion manipulation technique	1123:1160	a gas-phase ion manipulation technique (limited charge reduction)	1123:1187	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	53	theme	gas-phase	1125:1133	arg1	reduction					1178:1186	limited charge reduction	1163:1186	limited charge reduction	1163:1186	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	3	54	theme	mass	606:609	arg1	tool					645:648	a tool	643:648	a tool of choice	643:658	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	3	54	theme	mass	606:609	arg1	MS					625:626	MS	625:626	MS	625:626	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	3	54	theme	mass	606:609	arg1	spectrometry					611:622	Native mass spectrometry	599:622	Native mass spectrometry (MS)	599:627	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	9	55	from	information	2245:2255	arg1	attenuation					2260:2270	attenuation	2260:2270	attenuation of the ACE2/RBD association by heparin	2260:2309	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	1	56	theme	rapid	207:211	arg1	proliferation					213:225	rapid proliferation	207:225	rapid proliferation	207:225	The emergence and rapid proliferation of the novel coronavirus (SARS-CoV-2) resulted in a global pandemic, with over 6,000,000 cases and nearly 400,000 deaths reported worldwide by the end of May 2020.					
32678978	5	57	theme	cell	1611:1614	arg1	receptor					1616:1623	the host cell receptor	1602:1623	the host cell receptor	1602:1623	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32678978	9	58	theme	rational	2437:2444	arg1	repurposing					2446:2456	rational repurposing	2437:2456	rational repurposing of the existing medicines for treating COVID-19	2437:2504	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	0	59	theme	Host	169:172	arg1	Receptor					179:186	Its Host Cell Receptor	165:186	Its Host Cell Receptor	165:186	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	1	60	theme	novel	234:238	arg1	SARS-CoV-2					253:262	SARS-CoV-2	253:262	SARS-CoV-2	253:262	The emergence and rapid proliferation of the novel coronavirus (SARS-CoV-2) resulted in a global pandemic, with over 6,000,000 cases and nearly 400,000 deaths reported worldwide by the end of May 2020.					
32678978	1	60	theme	novel	234:238	arg1	coronavirus					240:250	the novel coronavirus	230:250	the novel coronavirus (SARS-CoV-2)	230:263	The emergence and rapid proliferation of the novel coronavirus (SARS-CoV-2) resulted in a global pandemic, with over 6,000,000 cases and nearly 400,000 deaths reported worldwide by the end of May 2020.					
32678978	7	61	theme	RBD	1904:1906	arg1	surface					1908:1914	the RBD surface	1900:1914	the RBD surface	1900:1914	This association alters the protein conformation (to maximize the contiguous patch of the positive charge on the RBD surface), resulting in a notable decrease in its ability to associate with ACE2.					
32678978	7	62	from	patch	1868:1872	arg1	surface					1908:1914	the RBD surface	1900:1914	the RBD surface	1900:1914	This association alters the protein conformation (to maximize the contiguous patch of the positive charge on the RBD surface), resulting in a notable decrease in its ability to associate with ACE2.					
32678978	8	63	theme	heparin	2017:2023	arg1	pronounced					2033:2042	pronounced	2033:2042	pronounced	2033:2042	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	8	63	theme	heparin	2017:2023	arg1	effect					2007:2012	The destabilizing effect	1989:2012	The destabilizing effect of heparin	1989:2023	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	6	64	theme	heparin	1694:1700	arg1	oligomers					1702:1710	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers	1626:1710	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers	1626:1710	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site.					
32678978	5	65	theme	ACE2/RBD	1447:1454	arg1	association					1456:1466	the ACE2/RBD association	1443:1466	the ACE2/RBD association to be studied	1443:1480	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32678978	9	66	theme	existing	2465:2472	arg1	medicines					2474:2482	the existing medicines	2461:2482	the existing medicines	2461:2482	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	2	67	theme	capable	563:569	arg1	tools					557:561	analytical tools	546:561	analytical tools capable of supporting such efforts	546:596	A rush to find a cure prompted re-evaluation of a range of existing therapeutics vis-à-vis their potential role in treating COVID-19, placing a premium on analytical tools capable of supporting such efforts.					
32678978	6	68	theme	binding	1777:1783	arg1	site					1785:1788	a single binding site	1768:1788	a single binding site	1768:1788	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site.					
32678978	4	69	theme	meaningful	1196:1205	arg1	information					1207:1217	meaningful information	1196:1217	meaningful information	1196:1217	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	9	70	theme	mechanistic	2233:2243	arg1	information					2245:2255	important mechanistic information	2223:2255	important mechanistic information on attenuation of the ACE2/RBD association by heparin	2223:2309	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	7	71	theme	positive	1881:1888	arg1	charge					1890:1895	the positive charge	1877:1895	the positive charge	1877:1895	This association alters the protein conformation (to maximize the contiguous patch of the positive charge on the RBD surface), resulting in a notable decrease in its ability to associate with ACE2.					
32678978	2	72	theme	analytical	546:555	arg1	tools					557:561	analytical tools	546:561	analytical tools capable of supporting such efforts	546:596	A rush to find a cure prompted re-evaluation of a range of existing therapeutics vis-à-vis their potential role in treating COVID-19, placing a premium on analytical tools capable of supporting such efforts.					
32678978	5	73	theme	molecular	1536:1544	arg1	mechanism					1546:1554	the molecular mechanism	1532:1554	the molecular mechanism of its interference with the virus docking to the host cell receptor	1532:1623	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32678978	2	74	theme	potential	488:496	arg1	role					498:501	their potential role	482:501	their potential role	482:501	A rush to find a cure prompted re-evaluation of a range of existing therapeutics vis-à-vis their potential role in treating COVID-19, placing a premium on analytical tools capable of supporting such efforts.					
32678978	4	75	theme	cell	994:997	arg1	surface					999:1005	the host cell surface	985:1005	the host cell surface	985:1005	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	7	76	theme	contiguous	1857:1866	arg1	patch					1868:1872	the contiguous patch	1853:1872	the contiguous patch of the positive charge on the RBD surface	1853:1914	This association alters the protein conformation (to maximize the contiguous patch of the positive charge on the RBD surface), resulting in a notable decrease in its ability to associate with ACE2.					
32678978	1	77	dep	emergence	193:201	arg1	The					189:191	The	189:191	The	189:191	The emergence and rapid proliferation of the novel coronavirus (SARS-CoV-2) resulted in a global pandemic, with over 6,000,000 cases and nearly 400,000 deaths reported worldwide by the end of May 2020.					
32678978	3	78	theme	drug/therapeutic	701:716	arg1	interactions					725:736	drug/therapeutic target interactions	701:736	drug/therapeutic target interactions	701:736	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	9	79	theme	ACE2/RBD	2279:2286	arg1	association					2288:2298	the ACE2/RBD association	2275:2298	the ACE2/RBD association	2275:2298	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	4	80	from	site	977:980	arg1	surface					999:1005	the host cell surface	985:1005	the host cell surface	985:1005	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	0	81	from	COVID-19	97:104	arg1	Mechanism					47:55	the Mechanism	43:55	the Mechanism of Action of Repurposed Therapeutics in COVID-19	43:104	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	8	82	theme	chains	2070:2075	arg1	case					2051:2054	the case	2047:2054	the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface	2047:2195	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	4	83	theme	native	1074:1079	arg1	MS.					1081:1083	native MS.	1074:1083	native MS.	1074:1083	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	0	84	theme	SARS-CoV-2	137:146	arg1	Interaction					148:158	the SARS-CoV-2 Interaction	133:158	the SARS-CoV-2 Interaction with Its Host Cell Receptor	133:186	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	1	85	theme	May	381:383	arg1	end					374:376	the end	370:376	the end of May 2020	370:388	The emergence and rapid proliferation of the novel coronavirus (SARS-CoV-2) resulted in a global pandemic, with over 6,000,000 cases and nearly 400,000 deaths reported worldwide by the end of May 2020.					
32678978	8	86	theme	electrostatic	2088:2100	arg1	repulsion					2102:2110	the electrostatic repulsion	2084:2110	the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface	2084:2195	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	3	87	theme	interactions	725:736	arg1	studies					690:696	the mechanistic studies	674:696	the mechanistic studies of drug/therapeutic target interactions	674:736	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	8	88	from	case	2051:2054	arg1	pronounced					2033:2042	pronounced	2033:2042	pronounced	2033:2042	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	8	88	from	case	2051:2054	arg1	effect					2007:2012	The destabilizing effect	1989:2012	The destabilizing effect of heparin	1989:2023	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	4	89	theme	receptor-binding	1286:1301	arg1	RBD					1311:1313	RBD	1311:1313	RBD	1311:1313	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	89	theme	receptor-binding	1286:1301	arg1	domain					1303:1308	the receptor-binding domain	1282:1308	the receptor-binding domain (RBD) of the S-protein	1282:1331	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	89	theme	receptor-binding	1286:1301	arg1	S-protein					1323:1331	the S-protein	1319:1331	the S-protein	1319:1331	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	0	90	theme	Therapeutics	81:92	arg1	Action					60:65	Action	60:65	Action of Repurposed Therapeutics in COVID-19	60:104	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	4	91	theme	spike	872:876	arg1	element					910:916	a critical element	899:916	a critical element of the viral entry to the host cell	899:952	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	91	theme	spike	872:876	arg1	S-protein					887:895	S-protein	887:895	S-protein	887:895	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	91	theme	spike	872:876	arg1	protein					878:884	SARS-CoV-2 spike protein	861:884	SARS-CoV-2 spike protein (S-protein)	861:896	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	91	theme	spike	872:876	arg1	site					977:980	its docking site	965:980	its docking site on the host cell surface	965:1005	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	5	92	theme	molecular	1375:1383	arg1	modeling					1385:1392	molecular modeling	1375:1392	molecular modeling	1375:1392	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32678978	2	93	theme	such	585:588	arg1	efforts					590:596	such efforts	585:596	such efforts	585:596	A rush to find a cure prompted re-evaluation of a range of existing therapeutics vis-à-vis their potential role in treating COVID-19, placing a premium on analytical tools capable of supporting such efforts.					
32678978	9	94	theme	untapped	2343:2350	arg1	potential					2352:2360	the yet untapped potential	2335:2360	the yet untapped potential of native MS coupled to gas-phase ion chemistry	2335:2408	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	9	94	theme	untapped	2343:2350	arg1	means					2415:2419	a means	2413:2419	a means of facilitating rational repurposing of the existing medicines for treating COVID-19	2413:2504	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	7	95	from	decrease	1941:1948	arg1	ability					1957:1963	its ability to associate with ACE2	1953:1986	its ability to associate with ACE2	1953:1986	This association alters the protein conformation (to maximize the contiguous patch of the positive charge on the RBD surface), resulting in a notable decrease in its ability to associate with ACE2.					
32678978	3	96	theme	structural	830:839	arg1	heterogeneity					841:853	structural heterogeneity	830:853	structural heterogeneity	830:853	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	5	97	theme	virus	1585:1589	arg1	docking					1591:1597	the virus docking	1581:1597	the virus docking to the host cell receptor	1581:1623	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32678978	2	98	dep	rush	393:396	arg1	find					401:404	find	401:404	to find a cure	398:411	A rush to find a cure prompted re-evaluation of a range of existing therapeutics vis-à-vis their potential role in treating COVID-19, placing a premium on analytical tools capable of supporting such efforts.					
32678978	4	99	theme	entry	931:935	arg1	element					910:916	a critical element	899:916	a critical element of the viral entry to the host cell	899:952	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	99	theme	entry	931:935	arg1	protein					878:884	SARS-CoV-2 spike protein	861:884	SARS-CoV-2 spike protein (S-protein)	861:896	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	100	theme	manipulation	1139:1150	arg1	technique					1152:1160	a gas-phase ion manipulation technique	1123:1160	a gas-phase ion manipulation technique (limited charge reduction)	1123:1187	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	100	theme	manipulation	1139:1150	arg1	reduction					1178:1186	limited charge reduction	1163:1186	limited charge reduction	1163:1186	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	0	101	theme	Native	15:20	arg1	MS					22:23	Native MS	15:23	Native MS	15:23	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	9	102	theme	MS	2372:2373	arg1	potential					2352:2360	the yet untapped potential	2335:2360	the yet untapped potential of native MS coupled to gas-phase ion chemistry	2335:2408	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	9	102	theme	MS	2372:2373	arg1	means					2415:2419	a means	2413:2419	a means of facilitating rational repurposing of the existing medicines for treating COVID-19	2413:2504	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	4	103	theme	limited	1163:1169	arg1	technique					1152:1160	a gas-phase ion manipulation technique	1123:1160	a gas-phase ion manipulation technique (limited charge reduction)	1123:1187	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	103	theme	limited	1163:1169	arg1	reduction					1178:1186	limited charge reduction	1163:1186	limited charge reduction	1163:1186	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	6	104	with	complexes	1721:1729	arg1	RBD					1736:1738	RBD	1736:1738	RBD	1736:1738	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site.					
32678978	9	105	theme	ion	2396:2398	arg1	chemistry					2400:2408	gas-phase ion chemistry	2386:2408	gas-phase ion chemistry	2386:2408	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	8	106	theme	low-pI	2124:2129	arg1	ACE2					2131:2134	low-pI ACE2	2124:2134	low-pI ACE2	2124:2134	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	9	107	dep	providing	2213:2221	arg1	addition					2201:2208	addition	2201:2208	addition	2201:2208	In addition to providing important mechanistic information on attenuation of the ACE2/RBD association by heparin, the study demonstrates the yet untapped potential of native MS coupled to gas-phase ion chemistry as a means of facilitating rational repurposing of the existing medicines for treating COVID-19.					
32678978	4	108	theme	ion	1135:1137	arg1	technique					1152:1160	a gas-phase ion manipulation technique	1123:1160	a gas-phase ion manipulation technique (limited charge reduction)	1123:1187	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	108	theme	ion	1135:1137	arg1	reduction					1178:1186	limited charge reduction	1163:1186	limited charge reduction	1163:1186	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	6	109	dep	long	1670:1673	arg1	eicosasaccharide					1676:1691	eicosasaccharide	1676:1691	eicosasaccharide	1676:1691	Both short (pentasaccharide) and relatively long (eicosasaccharide) heparin oligomers form 1:1 complexes with RBD, indicating the presence of a single binding site.					
32678978	3	110	theme	Native	599:604	arg1	tool					645:648	a tool	643:648	a tool of choice	643:658	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	3	110	theme	Native	599:604	arg1	MS					625:626	MS	625:626	MS	625:626	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	3	110	theme	Native	599:604	arg1	spectrometry					611:622	Native mass spectrometry	599:622	Native mass spectrometry (MS)	599:627	Native mass spectrometry (MS) has long been a tool of choice in supporting the mechanistic studies of drug/therapeutic target interactions, but its applications remain limited in the cases that involve systems with a high level of structural heterogeneity.					
32678978	8	111	theme	heparin	2144:2150	arg1	segments					2152:2159	the low-pI ACE2 and the heparin segments	2120:2159	the low-pI ACE2 and the heparin segments not accommodated on the RBD surface	2120:2195	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	0	112	dep	Utility	4:10	arg1	Heparin					107:113	Heparin	107:113	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.	0:187	The Utility of Native MS for Understanding the Mechanism of Action of Repurposed Therapeutics in COVID-19: Heparin as a Disruptor of the SARS-CoV-2 Interaction with Its Host Cell Receptor.					
32678978	8	113	theme	destabilizing	1993:2005	arg1	pronounced					2033:2042	pronounced	2033:2042	pronounced	2033:2042	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	8	113	theme	destabilizing	1993:2005	arg1	effect					2007:2012	The destabilizing effect	1989:2012	The destabilizing effect of heparin	1989:2023	The destabilizing effect of heparin is more pronounced in the case of the longer chains due to the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the RBD surface.					
32678978	4	114	theme	docking	969:975	arg1	protein					878:884	SARS-CoV-2 spike protein	861:884	SARS-CoV-2 spike protein (S-protein)	861:896	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	4	114	theme	docking	969:975	arg1	site					977:980	its docking site	965:980	its docking site on the host cell surface	965:1005	Both SARS-CoV-2 spike protein (S-protein), a critical element of the viral entry to the host cell, and ACE2, its docking site on the host cell surface, are extensively glycosylated, making them challenging targets for native MS. However, supplementing native MS with a gas-phase ion manipulation technique (limited charge reduction) allows meaningful information to be obtained on the noncovalent complexes formed by ACE2 and the receptor-binding domain (RBD) of the S-protein.					
32678978	5	115	theme	host	1606:1609	arg1	receptor					1616:1623	the host cell receptor	1602:1623	the host cell receptor	1602:1623	Using this technique in combination with molecular modeling also allows the role of heparin in destabilizing the ACE2/RBD association to be studied, providing critical information for understanding the molecular mechanism of its interference with the virus docking to the host cell receptor.					
32142841	2	0	theme	slight	454:459	arg1	brown					461:465	slight brown	454:465	slight brown in color	454:474	The composite films were flexible and transparent or translucent with slight brown in color.					
32142841	1	1	theme	binary	281:286	arg1	composites					312:321	lignin (L)-based binary and ternary sustainable composites	264:321	lignin (L)-based binary and ternary sustainable composites	264:321	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	0	2	theme	water	171:175	arg1	properties					185:194	water barrier properties	171:194	water barrier properties	171:194	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	6	3	theme	composite	924:932	arg1	films					934:938	the composite films	920:938	the composite films	920:938	The surface hydrophobicity of the composite films increased with the addition of lignin.					
32142841	0	4	theme	cellulose	70:78	arg1	activity					154:161	antioxidant activity	142:161	antioxidant activity	142:161	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	4	theme	cellulose	70:78	arg1	UV					164:165	UV	164:165	UV	164:165	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	4	theme	cellulose	70:78	arg1	cellulose					70:78	hydroxyethyl cellulose	57:78	hydroxyethyl cellulose	57:78	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	4	theme	cellulose	70:78	arg1	composites					31:40	Binary and ternary sustainable composites	0:40	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.	0:195	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	4	theme	cellulose	70:78	arg1	Biocompatibility					124:139	Biocompatibility	124:139	Biocompatibility	124:139	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	4	theme	cellulose	70:78	arg1	lignin					84:89	lignin	84:89	lignin	84:89	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	4	theme	cellulose	70:78	arg1	gum					52:54	gellan gum	45:54	gellan gum	45:54	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	9	5	theme	non-cytotoxic	1240:1252	arg1	activities					1254:1263	efficient antioxidant and non-cytotoxic activities	1214:1263	efficient antioxidant and non-cytotoxic activities	1214:1263	The obtained composites showed efficient antioxidant and non-cytotoxic activities, although there was no remarkable antimicrobial activity.					
32142841	1	6	theme	2-hydroxyethyl	228:241	arg1	HEC					254:256	HEC	254:256	HEC	254:256	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	1	6	theme	2-hydroxyethyl	228:241	arg1	cellulose					243:251	2-hydroxyethyl cellulose	228:251	2-hydroxyethyl cellulose (HEC)	228:257	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	7	7	theme	radical	1068:1074	arg1	activities					1087:1096	excellent radical scavenging activities	1058:1096	excellent radical scavenging activities	1058:1096	The binary and ternary composites containing 1, 5, and 10 wt% lignin exhibited excellent radical scavenging activities.					
32142841	1	8	theme	ternary	292:298	arg1	composites					312:321	lignin (L)-based binary and ternary sustainable composites	264:321	lignin (L)-based binary and ternary sustainable composites	264:321	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	1	9	theme	cellulose	243:251	arg1	Biopolymers					197:207	Biopolymers	197:207	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites	197:321	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	0	10	theme	lignin	84:89	arg1	activity					154:161	antioxidant activity	142:161	antioxidant activity	142:161	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	10	theme	lignin	84:89	arg1	UV					164:165	UV	164:165	UV	164:165	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	10	theme	lignin	84:89	arg1	cellulose					70:78	hydroxyethyl cellulose	57:78	hydroxyethyl cellulose	57:78	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	10	theme	lignin	84:89	arg1	composites					31:40	Binary and ternary sustainable composites	0:40	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.	0:195	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	10	theme	lignin	84:89	arg1	Biocompatibility					124:139	Biocompatibility	124:139	Biocompatibility	124:139	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	10	theme	lignin	84:89	arg1	lignin					84:89	lignin	84:89	lignin	84:89	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	10	theme	lignin	84:89	arg1	gum					52:54	gellan gum	45:54	gellan gum	45:54	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	1	11	theme	sustainable	300:310	arg1	composites					312:321	lignin (L)-based binary and ternary sustainable composites	264:321	lignin (L)-based binary and ternary sustainable composites	264:321	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	0	12	theme	barrier	177:183	arg1	properties					185:194	water barrier properties	171:194	water barrier properties	171:194	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	8	13	theme	gellan	1103:1108	arg1	films					1141:1145	The gellan gum/HEC/lignin based composite films	1099:1145	The gellan gum/HEC/lignin based composite films	1099:1145	The gellan gum/HEC/lignin based composite films achieved the best biocompatibility.					
32142841	0	14	theme	packaging	100:108	arg1	applications					110:121	food packaging applications	95:121	food packaging applications	95:121	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	1	15	theme	composites	312:321	arg1	Biopolymers					197:207	Biopolymers	197:207	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites	197:321	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	7	16	dep	%	1039:1039	arg1	5					1027:1027	5	1027:1027	5	1027:1027	The binary and ternary composites containing 1, 5, and 10 wt% lignin exhibited excellent radical scavenging activities.					
32142841	7	16	dep	%	1039:1039	arg1	10 wt					1034:1038	10 wt	1034:1038	10 wt	1034:1038	The binary and ternary composites containing 1, 5, and 10 wt% lignin exhibited excellent radical scavenging activities.					
32142841	8	17	theme	gum/HEC/lignin	1110:1123	arg1	films					1141:1145	The gellan gum/HEC/lignin based composite films	1099:1145	The gellan gum/HEC/lignin based composite films	1099:1145	The gellan gum/HEC/lignin based composite films achieved the best biocompatibility.					
32142841	0	18	theme	food	95:98	arg1	applications					110:121	food packaging applications	95:121	food packaging applications	95:121	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	7	19	dep	lignin	1041:1046	arg1	%					1039:1039	%	1039:1039	%	1039:1039	The binary and ternary composites containing 1, 5, and 10 wt% lignin exhibited excellent radical scavenging activities.					
32142841	3	20	theme	lignin	498:503	arg1	incorporation					481:493	The incorporation	477:493	The incorporation of lignin	477:503	The incorporation of lignin considerably improved the thermal and mechanical and hydrophobic properties of the composite films.					
32142841	4	21	theme	%	626:626	arg1	addition					609:616	The addition	605:616	The addition of 10 wt% of lignin to the composites	605:654	The addition of 10 wt% of lignin to the composites increased the tensile strength by 54.3% and 59.2% respectively.					
32142841	5	22	theme	high	769:772	arg1	protection					791:800	high ultraviolet (UV) protection	769:800	high ultraviolet (UV) protection	769:800	The prepared lignin-based composite films showed high ultraviolet (UV) protection, with almost 100% protection against UVB (280-320 nm) and 90% against UVA (320-400 nm).					
32142841	5	23	theme	prepared	724:731	arg1	films					756:760	The prepared lignin-based composite films	720:760	The prepared lignin-based composite films	720:760	The prepared lignin-based composite films showed high ultraviolet (UV) protection, with almost 100% protection against UVB (280-320 nm) and 90% against UVA (320-400 nm).					
32142841	0	24	theme	Binary	0:5	arg1	activity					154:161	antioxidant activity	142:161	antioxidant activity	142:161	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	24	theme	Binary	0:5	arg1	UV					164:165	UV	164:165	UV	164:165	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	24	theme	Binary	0:5	arg1	cellulose					70:78	hydroxyethyl cellulose	57:78	hydroxyethyl cellulose	57:78	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	24	theme	Binary	0:5	arg1	composites					31:40	Binary and ternary sustainable composites	0:40	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.	0:195	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	24	theme	Binary	0:5	arg1	Biocompatibility					124:139	Biocompatibility	124:139	Biocompatibility	124:139	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	24	theme	Binary	0:5	arg1	lignin					84:89	lignin	84:89	lignin	84:89	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	24	theme	Binary	0:5	arg1	gum					52:54	gellan gum	45:54	gellan gum	45:54	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	6	25	theme	surface	894:900	arg1	hydrophobicity					902:915	The surface hydrophobicity	890:915	The surface hydrophobicity of the composite films	890:938	The surface hydrophobicity of the composite films increased with the addition of lignin.					
32142841	3	26	theme	mechanical	543:552	arg1	properties					570:579	the thermal and mechanical and hydrophobic properties	527:579	the thermal and mechanical and hydrophobic properties of the composite films	527:602	The incorporation of lignin considerably improved the thermal and mechanical and hydrophobic properties of the composite films.					
32142841	4	27	theme	lignin	631:636	arg1	lignin					631:636	lignin	631:636	lignin	631:636	The addition of 10 wt% of lignin to the composites increased the tensile strength by 54.3% and 59.2% respectively.					
32142841	4	27	theme	lignin	631:636	arg1	%					626:626	10 wt%	621:626	10 wt% of lignin	621:636	The addition of 10 wt% of lignin to the composites increased the tensile strength by 54.3% and 59.2% respectively.					
32142841	0	28	theme	sustainable	19:29	arg1	activity					154:161	antioxidant activity	142:161	antioxidant activity	142:161	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	28	theme	sustainable	19:29	arg1	UV					164:165	UV	164:165	UV	164:165	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	28	theme	sustainable	19:29	arg1	cellulose					70:78	hydroxyethyl cellulose	57:78	hydroxyethyl cellulose	57:78	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	28	theme	sustainable	19:29	arg1	composites					31:40	Binary and ternary sustainable composites	0:40	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.	0:195	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	28	theme	sustainable	19:29	arg1	Biocompatibility					124:139	Biocompatibility	124:139	Biocompatibility	124:139	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	28	theme	sustainable	19:29	arg1	lignin					84:89	lignin	84:89	lignin	84:89	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	28	theme	sustainable	19:29	arg1	gum					52:54	gellan gum	45:54	gellan gum	45:54	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	5	29	theme	ultraviolet	774:784	arg1	protection					791:800	high ultraviolet (UV) protection	769:800	high ultraviolet (UV) protection	769:800	The prepared lignin-based composite films showed high ultraviolet (UV) protection, with almost 100% protection against UVB (280-320 nm) and 90% against UVA (320-400 nm).					
32142841	3	30	theme	hydrophobic	558:568	arg1	properties					570:579	the thermal and mechanical and hydrophobic properties	527:579	the thermal and mechanical and hydrophobic properties of the composite films	527:602	The incorporation of lignin considerably improved the thermal and mechanical and hydrophobic properties of the composite films.					
32142841	8	31	theme	based	1125:1129	arg1	films					1141:1145	The gellan gum/HEC/lignin based composite films	1099:1145	The gellan gum/HEC/lignin based composite films	1099:1145	The gellan gum/HEC/lignin based composite films achieved the best biocompatibility.					
32142841	0	32	theme	ternary	11:17	arg1	activity					154:161	antioxidant activity	142:161	antioxidant activity	142:161	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	32	theme	ternary	11:17	arg1	UV					164:165	UV	164:165	UV	164:165	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	32	theme	ternary	11:17	arg1	cellulose					70:78	hydroxyethyl cellulose	57:78	hydroxyethyl cellulose	57:78	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	32	theme	ternary	11:17	arg1	composites					31:40	Binary and ternary sustainable composites	0:40	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.	0:195	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	32	theme	ternary	11:17	arg1	Biocompatibility					124:139	Biocompatibility	124:139	Biocompatibility	124:139	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	32	theme	ternary	11:17	arg1	lignin					84:89	lignin	84:89	lignin	84:89	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	32	theme	ternary	11:17	arg1	gum					52:54	gellan gum	45:54	gellan gum	45:54	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	9	33	theme	antimicrobial	1299:1311	arg1	activity					1313:1320	no remarkable antimicrobial activity	1285:1320	no remarkable antimicrobial activity	1285:1320	The obtained composites showed efficient antioxidant and non-cytotoxic activities, although there was no remarkable antimicrobial activity.					
32142841	7	34	theme	ternary	994:1000	arg1	composites					1002:1011	The binary and ternary composites	979:1011	The binary and ternary composites containing 1, 5, and 10 wt% lignin	979:1046	The binary and ternary composites containing 1, 5, and 10 wt% lignin exhibited excellent radical scavenging activities.					
32142841	3	35	theme	thermal	531:537	arg1	properties					570:579	the thermal and mechanical and hydrophobic properties	527:579	the thermal and mechanical and hydrophobic properties of the composite films	527:602	The incorporation of lignin considerably improved the thermal and mechanical and hydrophobic properties of the composite films.					
32142841	6	36	theme	lignin	971:976	arg1	addition					959:966	the addition	955:966	the addition of lignin	955:976	The surface hydrophobicity of the composite films increased with the addition of lignin.					
32142841	5	37	theme	lignin-based	733:744	arg1	films					756:760	The prepared lignin-based composite films	720:760	The prepared lignin-based composite films	720:760	The prepared lignin-based composite films showed high ultraviolet (UV) protection, with almost 100% protection against UVB (280-320 nm) and 90% against UVA (320-400 nm).					
32142841	1	38	theme	food	341:344	arg1	packaging					346:354	food packaging	341:354	food packaging	341:354	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	5	39	dep	%	818:818	arg1	100					815:817	100	815:817	100	815:817	The prepared lignin-based composite films showed high ultraviolet (UV) protection, with almost 100% protection against UVB (280-320 nm) and 90% against UVA (320-400 nm).					
32142841	9	40	theme	remarkable	1288:1297	arg1	activity					1313:1320	no remarkable antimicrobial activity	1285:1320	no remarkable antimicrobial activity	1285:1320	The obtained composites showed efficient antioxidant and non-cytotoxic activities, although there was no remarkable antimicrobial activity.					
32142841	5	41	theme	composite	746:754	arg1	films					756:760	The prepared lignin-based composite films	720:760	The prepared lignin-based composite films	720:760	The prepared lignin-based composite films showed high ultraviolet (UV) protection, with almost 100% protection against UVB (280-320 nm) and 90% against UVA (320-400 nm).					
32142841	7	42	theme	excellent	1058:1066	arg1	activities					1087:1096	excellent radical scavenging activities	1058:1096	excellent radical scavenging activities	1058:1096	The binary and ternary composites containing 1, 5, and 10 wt% lignin exhibited excellent radical scavenging activities.					
32142841	5	43	dep	protection	820:829	arg1	%					818:818	%	818:818	%	818:818	The prepared lignin-based composite films showed high ultraviolet (UV) protection, with almost 100% protection against UVB (280-320 nm) and 90% against UVA (320-400 nm).					
32142841	1	44	theme	gellan	212:217	arg1	G					224:224	G	224:224	G	224:224	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	1	44	theme	gellan	212:217	arg1	gum					219:221	gellan gum	212:221	gellan gum (G)	212:225	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	0	45	theme	gum	52:54	arg1	activity					154:161	antioxidant activity	142:161	antioxidant activity	142:161	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	45	theme	gum	52:54	arg1	UV					164:165	UV	164:165	UV	164:165	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	45	theme	gum	52:54	arg1	cellulose					70:78	hydroxyethyl cellulose	57:78	hydroxyethyl cellulose	57:78	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	45	theme	gum	52:54	arg1	composites					31:40	Binary and ternary sustainable composites	0:40	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.	0:195	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	45	theme	gum	52:54	arg1	Biocompatibility					124:139	Biocompatibility	124:139	Biocompatibility	124:139	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	45	theme	gum	52:54	arg1	lignin					84:89	lignin	84:89	lignin	84:89	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	45	theme	gum	52:54	arg1	gum					52:54	gellan gum	45:54	gellan gum	45:54	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	8	46	theme	composite	1131:1139	arg1	films					1141:1145	The gellan gum/HEC/lignin based composite films	1099:1145	The gellan gum/HEC/lignin based composite films	1099:1145	The gellan gum/HEC/lignin based composite films achieved the best biocompatibility.					
32142841	2	47	from	brown	461:465	arg1	color					470:474	color	470:474	color	470:474	The composite films were flexible and transparent or translucent with slight brown in color.					
32142841	7	48	theme	binary	983:988	arg1	composites					1002:1011	The binary and ternary composites	979:1011	The binary and ternary composites containing 1, 5, and 10 wt% lignin	979:1046	The binary and ternary composites containing 1, 5, and 10 wt% lignin exhibited excellent radical scavenging activities.					
32142841	0	49	theme	gellan	45:50	arg1	gum					52:54	gellan gum	45:54	gellan gum	45:54	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	9	50	theme	obtained	1187:1194	arg1	composites					1196:1205	The obtained composites	1183:1205	The obtained composites	1183:1205	The obtained composites showed efficient antioxidant and non-cytotoxic activities, although there was no remarkable antimicrobial activity.					
32142841	0	51	theme	antioxidant	142:152	arg1	cellulose					70:78	hydroxyethyl cellulose	57:78	hydroxyethyl cellulose	57:78	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	51	theme	antioxidant	142:152	arg1	activity					154:161	antioxidant activity	142:161	antioxidant activity	142:161	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	51	theme	antioxidant	142:152	arg1	lignin					84:89	lignin	84:89	lignin	84:89	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	51	theme	antioxidant	142:152	arg1	composites					31:40	Binary and ternary sustainable composites	0:40	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.	0:195	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	51	theme	antioxidant	142:152	arg1	gum					52:54	gellan gum	45:54	gellan gum	45:54	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	1	52	theme	biomedical	360:369	arg1	application					371:381	biomedical application	360:381	biomedical application	360:381	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	0	53	dep	composites	31:40	arg1	properties					185:194	water barrier properties	171:194	water barrier properties	171:194	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	53	dep	composites	31:40	arg1	activity					154:161	antioxidant activity	142:161	antioxidant activity	142:161	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	53	dep	composites	31:40	arg1	UV					164:165	UV	164:165	UV	164:165	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	53	dep	composites	31:40	arg1	cellulose					70:78	hydroxyethyl cellulose	57:78	hydroxyethyl cellulose	57:78	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	53	dep	composites	31:40	arg1	composites					31:40	Binary and ternary sustainable composites	0:40	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.	0:195	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	53	dep	composites	31:40	arg1	Biocompatibility					124:139	Biocompatibility	124:139	Biocompatibility	124:139	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	53	dep	composites	31:40	arg1	lignin					84:89	lignin	84:89	lignin	84:89	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	0	53	dep	composites	31:40	arg1	gum					52:54	gellan gum	45:54	gellan gum	45:54	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	2	54	with	flexible	409:416	arg1	brown					461:465	slight brown	454:465	slight brown in color	454:474	The composite films were flexible and transparent or translucent with slight brown in color.					
32142841	4	55	theme	tensile	670:676	arg1	strength					678:685	the tensile strength	666:685	the tensile strength	666:685	The addition of 10 wt% of lignin to the composites increased the tensile strength by 54.3% and 59.2% respectively.					
32142841	7	56	contain	containing	1013:1022	arg1	composites					1002:1011	The binary and ternary composites	979:1011	The binary and ternary composites containing 1, 5, and 10 wt% lignin	979:1046	The binary and ternary composites containing 1, 5, and 10 wt% lignin exhibited excellent radical scavenging activities.					
32142841	7	56	contain	containing	1013:1022	arg2	lignin					1041:1046	1, 5, and 10 wt% lignin	1024:1046	1, 5, and 10 wt% lignin	1024:1046	The binary and ternary composites containing 1, 5, and 10 wt% lignin exhibited excellent radical scavenging activities.					
32142841	3	57	theme	composite	588:596	arg1	films					598:602	the composite films	584:602	the composite films	584:602	The incorporation of lignin considerably improved the thermal and mechanical and hydrophobic properties of the composite films.					
32142841	3	58	theme	films	598:602	arg1	properties					570:579	the thermal and mechanical and hydrophobic properties	527:579	the thermal and mechanical and hydrophobic properties of the composite films	527:602	The incorporation of lignin considerably improved the thermal and mechanical and hydrophobic properties of the composite films.					
32142841	1	59	theme	gum	219:221	arg1	Biopolymers					197:207	Biopolymers	197:207	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites	197:321	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	5	60	theme	UV	787:788	arg1	protection					791:800	high ultraviolet (UV) protection	769:800	high ultraviolet (UV) protection	769:800	The prepared lignin-based composite films showed high ultraviolet (UV) protection, with almost 100% protection against UVB (280-320 nm) and 90% against UVA (320-400 nm).					
32142841	0	61	theme	hydroxyethyl	57:68	arg1	cellulose					70:78	hydroxyethyl cellulose	57:78	hydroxyethyl cellulose	57:78	Binary and ternary sustainable composites of gellan gum, hydroxyethyl cellulose and lignin for food packaging applications: Biocompatibility, antioxidant activity, UV and water barrier properties.					
32142841	9	62	theme	efficient	1214:1222	arg1	activities					1254:1263	efficient antioxidant and non-cytotoxic activities	1214:1263	efficient antioxidant and non-cytotoxic activities	1214:1263	The obtained composites showed efficient antioxidant and non-cytotoxic activities, although there was no remarkable antimicrobial activity.					
32142841	8	63	theme	best	1160:1163	arg1	biocompatibility					1165:1180	the best biocompatibility	1156:1180	the best biocompatibility	1156:1180	The gellan gum/HEC/lignin based composite films achieved the best biocompatibility.					
32142841	1	64	theme	-based	274:279	arg1	composites					312:321	lignin (L)-based binary and ternary sustainable composites	264:321	lignin (L)-based binary and ternary sustainable composites	264:321	Biopolymers of gellan gum (G), 2-hydroxyethyl cellulose (HEC), and lignin (L)-based binary and ternary sustainable composites were prepared for food packaging and biomedical application.					
32142841	2	65	theme	composite	388:396	arg1	flexible					409:416	flexible	409:416	flexible	409:416	The composite films were flexible and transparent or translucent with slight brown in color.					
32142841	2	65	theme	composite	388:396	arg1	films					398:402	The composite films	384:402	The composite films	384:402	The composite films were flexible and transparent or translucent with slight brown in color.					
32142841	9	66	theme	antioxidant	1224:1234	arg1	activities					1254:1263	efficient antioxidant and non-cytotoxic activities	1214:1263	efficient antioxidant and non-cytotoxic activities	1214:1263	The obtained composites showed efficient antioxidant and non-cytotoxic activities, although there was no remarkable antimicrobial activity.					
32142841	7	67	theme	scavenging	1076:1085	arg1	activities					1087:1096	excellent radical scavenging activities	1058:1096	excellent radical scavenging activities	1058:1096	The binary and ternary composites containing 1, 5, and 10 wt% lignin exhibited excellent radical scavenging activities.					
32142841	6	68	theme	films	934:938	arg1	hydrophobicity					902:915	The surface hydrophobicity	890:915	The surface hydrophobicity of the composite films	890:938	The surface hydrophobicity of the composite films increased with the addition of lignin.					
32526222	0	0	theme	novel	90:94	arg1	roles					96:100	novel roles	90:100	novel roles	90:100	Unique glycan and lipid composition of helminth-derived extracellular vesicles may reveal novel roles in host-parasite interactions.					
32526222	1	1	theme	EV	342:343	arg1	proteins					345:352	mammalian EV proteins	332:352	mammalian EV proteins including components of the endosomal sorting complexes required for transport	332:431	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	1	theme	EV	342:343	arg1	components					364:373	components	364:373	components of the endosomal sorting complexes required for transport	364:431	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	3	2	theme	Heligmosomoides	779:793	arg1	polygyrus					795:803	Heligmosomoides polygyrus	779:803	Heligmosomoides polygyrus	779:803	This opinion article highlights two exceptional studies that identified EVs released by Heligmosomoides polygyrus and Fasciola hepatica which display differential lipid and glycan composition, respectively, when compared with EVs derived from mammalian cells.					
32526222	2	3	theme	helminth	583:590	arg1	biology					592:598	parasitic helminth biology	573:598	parasitic helminth biology	573:598	However, parasitic helminth biology is unique and this is likely reflected in helminth EV composition and biological activity.					
32526222	1	4	theme	extracellular	172:184	arg1	EVs					196:198	EVs	196:198	EVs	196:198	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	4	theme	extracellular	172:184	arg1	vesicles					186:193	helminth-derived extracellular vesicles	155:193	helminth-derived extracellular vesicles (EVs)	155:199	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	4	5	theme	composition	1034:1044	arg1	implications					989:1000	the potential implications	975:1000	the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology	975:1087	Furthermore, we discuss the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology.					
32526222	1	6	theme	proteins	345:352	arg1	homologs					320:327	homologs	320:327	homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport	320:431	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	6	theme	proteins	345:352	arg1	proteins					448:455	heat-shock proteins	437:455	heat-shock proteins	437:455	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	7	theme	vesicles	186:193	arg1	study					146:150	the study	142:150	the study of helminth-derived extracellular vesicles (EVs)	142:199	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	5	8	theme	EVs	1179:1181	arg1	composition					1129:1139	unique composition	1122:1139	unique composition	1122:1139	Future studies, focusing on the unique composition and functional properties of helminth EVs, may prove crucial to the understanding of host-parasite communication.					
32526222	5	8	theme	EVs	1179:1181	arg1	properties					1156:1165	functional properties	1145:1165	functional properties	1145:1165	Future studies, focusing on the unique composition and functional properties of helminth EVs, may prove crucial to the understanding of host-parasite communication.					
32526222	5	9	theme	unique	1122:1127	arg1	composition					1129:1139	unique composition	1122:1139	unique composition	1122:1139	Future studies, focusing on the unique composition and functional properties of helminth EVs, may prove crucial to the understanding of host-parasite communication.					
32526222	4	10	theme	helminth	1005:1012	arg1	lipid					1017:1021	helminth EV lipid	1005:1021	helminth EV lipid	1005:1021	Furthermore, we discuss the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology.					
32526222	2	11	theme	biological	670:679	arg1	activity					681:688	biological activity	670:688	biological activity	670:688	However, parasitic helminth biology is unique and this is likely reflected in helminth EV composition and biological activity.					
32526222	4	12	theme	host	1074:1077	arg1	pathology					1079:1087	host pathology	1074:1087	host pathology	1074:1087	Furthermore, we discuss the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology.					
32526222	1	13	theme	nematodes	269:277	arg1	representatives					250:264	representatives	250:264	representatives of nematodes, cestodes and trematodes	250:302	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	4	14	theme	lipid	1017:1021	arg1	implications					989:1000	the potential implications	975:1000	the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology	975:1087	Furthermore, we discuss the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology.					
32526222	2	15	theme	EV	651:652	arg1	composition					654:664	helminth EV composition	642:664	helminth EV composition	642:664	However, parasitic helminth biology is unique and this is likely reflected in helminth EV composition and biological activity.					
32526222	5	16	theme	functional	1145:1154	arg1	properties					1156:1165	functional properties	1145:1165	functional properties	1145:1165	Future studies, focusing on the unique composition and functional properties of helminth EVs, may prove crucial to the understanding of host-parasite communication.					
32526222	0	17	theme	host-parasite	105:117	arg1	interactions					119:130	host-parasite interactions	105:130	host-parasite interactions	105:130	Unique glycan and lipid composition of helminth-derived extracellular vesicles may reveal novel roles in host-parasite interactions.					
32526222	5	18	theme	helminth	1170:1177	arg1	EVs					1179:1181	helminth EVs	1170:1181	helminth EVs	1170:1181	Future studies, focusing on the unique composition and functional properties of helminth EVs, may prove crucial to the understanding of host-parasite communication.					
32526222	2	19	theme	helminth	642:649	arg1	composition					654:664	helminth EV composition	642:664	helminth EV composition	642:664	However, parasitic helminth biology is unique and this is likely reflected in helminth EV composition and biological activity.					
32526222	1	20	theme	pathways	485:492	arg1	conservation					469:480	conservation	469:480	conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts	469:561	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	21	from	representatives	250:264	arg1	EVs					241:243	EVs	241:243	EVs from representatives of nematodes, cestodes and trematodes	241:302	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	21	from	representatives	250:264	arg1	studies					230:236	proteomic studies	220:236	proteomic studies of EVs from representatives of nematodes, cestodes and trematodes	220:302	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	22	theme	endosomal	382:390	arg1	complexes					400:408	the endosomal sorting complexes	378:408	the endosomal sorting complexes required for transport	378:431	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	0	23	link	helminth-derived	39:54	arg1	vesicles					70:77	helminth-derived extracellular vesicles	39:77	helminth-derived extracellular vesicles	39:77	Unique glycan and lipid composition of helminth-derived extracellular vesicles may reveal novel roles in host-parasite interactions.					
32526222	0	24	theme	Unique	0:5	arg1	glycan					7:12	Unique glycan	0:12	Unique glycan	0:12	Unique glycan and lipid composition of helminth-derived extracellular vesicles may reveal novel roles in host-parasite interactions.					
32526222	5	25	theme	host-parasite	1226:1238	arg1	communication					1240:1252	host-parasite communication	1226:1252	host-parasite communication	1226:1252	Future studies, focusing on the unique composition and functional properties of helminth EVs, may prove crucial to the understanding of host-parasite communication.					
32526222	1	26	theme	EV	497:498	arg1	biogenesis					500:509	EV biogenesis	497:509	EV biogenesis	497:509	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	0	27	theme	lipid	18:22	arg1	composition					24:34	lipid composition	18:34	lipid composition	18:34	Unique glycan and lipid composition of helminth-derived extracellular vesicles may reveal novel roles in host-parasite interactions.					
32526222	1	28	theme	cestodes	280:287	arg1	representatives					250:264	representatives	250:264	representatives of nematodes, cestodes and trematodes	250:302	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	2	29	theme	parasitic	573:581	arg1	biology					592:598	parasitic helminth biology	573:598	parasitic helminth biology	573:598	However, parasitic helminth biology is unique and this is likely reflected in helminth EV composition and biological activity.					
32526222	1	30	theme	biogenesis	500:509	arg1	pathways					485:492	pathways	485:492	pathways of EV biogenesis and cargo loading	485:527	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	31	theme	sorting	392:398	arg1	complexes					400:408	the endosomal sorting complexes	378:408	the endosomal sorting complexes required for transport	378:431	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	32	theme	trematodes	293:302	arg1	representatives					250:264	representatives	250:264	representatives of nematodes, cestodes and trematodes	250:302	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	33	theme	complexes	400:408	arg1	components					364:373	components	364:373	components of the endosomal sorting complexes required for transport	364:431	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	4	34	theme	potential	979:987	arg1	implications					989:1000	the potential implications	975:1000	the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology	975:1087	Furthermore, we discuss the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology.					
32526222	4	35	theme	helminth	1051:1058	arg1	infection					1060:1068	helminth infection	1051:1068	helminth infection	1051:1068	Furthermore, we discuss the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology.					
32526222	1	36	link	helminth-derived	155:170	arg1	EVs					196:198	EVs	196:198	EVs	196:198	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	36	link	helminth-derived	155:170	arg1	vesicles					186:193	helminth-derived extracellular vesicles	155:193	helminth-derived extracellular vesicles (EVs)	155:199	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	4	37	theme	EV	1014:1015	arg1	lipid					1017:1021	helminth EV lipid	1005:1021	helminth EV lipid	1005:1021	Furthermore, we discuss the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology.					
32526222	0	38	theme	extracellular	56:68	arg1	vesicles					70:77	helminth-derived extracellular vesicles	39:77	helminth-derived extracellular vesicles	39:77	Unique glycan and lipid composition of helminth-derived extracellular vesicles may reveal novel roles in host-parasite interactions.					
32526222	5	39	theme	communication	1240:1252	arg1	understanding					1209:1221	the understanding	1205:1221	the understanding of host-parasite communication	1205:1252	Future studies, focusing on the unique composition and functional properties of helminth EVs, may prove crucial to the understanding of host-parasite communication.					
32526222	5	40	theme	Future	1090:1095	arg1	studies					1097:1103	Future studies	1090:1103	Future studies	1090:1103	Future studies, focusing on the unique composition and functional properties of helminth EVs, may prove crucial to the understanding of host-parasite communication.					
32526222	1	41	theme	cargo	515:519	arg1	loading					521:527	cargo loading	515:527	cargo loading	515:527	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	0	42	theme	helminth-derived	39:54	arg1	vesicles					70:77	helminth-derived extracellular vesicles	39:77	helminth-derived extracellular vesicles	39:77	Unique glycan and lipid composition of helminth-derived extracellular vesicles may reveal novel roles in host-parasite interactions.					
32526222	3	43	theme	Fasciola	809:816	arg1	hepatica					818:825	Fasciola hepatica	809:825	Fasciola hepatica	809:825	This opinion article highlights two exceptional studies that identified EVs released by Heligmosomoides polygyrus and Fasciola hepatica which display differential lipid and glycan composition, respectively, when compared with EVs derived from mammalian cells.					
32526222	3	44	theme	exceptional	727:737	arg1	studies					739:745	two exceptional studies	723:745	two exceptional studies that identified EVs released by Heligmosomoides polygyrus and Fasciola hepatica which display differential lipid and glycan composition, respectively, when compared with EVs derived from mammalian cells	723:948	This opinion article highlights two exceptional studies that identified EVs released by Heligmosomoides polygyrus and Fasciola hepatica which display differential lipid and glycan composition, respectively, when compared with EVs derived from mammalian cells.					
32526222	3	45	theme	opinion	696:702	arg1	article					704:710	This opinion article	691:710	This opinion article	691:710	This opinion article highlights two exceptional studies that identified EVs released by Heligmosomoides polygyrus and Fasciola hepatica which display differential lipid and glycan composition, respectively, when compared with EVs derived from mammalian cells.					
32526222	3	46	attach	derived	921:927	arg2	EVs					917:919	EVs	917:919	EVs derived from mammalian cells	917:948	This opinion article highlights two exceptional studies that identified EVs released by Heligmosomoides polygyrus and Fasciola hepatica which display differential lipid and glycan composition, respectively, when compared with EVs derived from mammalian cells.					
32526222	3	46	attach	derived	921:927	arg1	cells					944:948	mammalian cells	934:948	mammalian cells	934:948	This opinion article highlights two exceptional studies that identified EVs released by Heligmosomoides polygyrus and Fasciola hepatica which display differential lipid and glycan composition, respectively, when compared with EVs derived from mammalian cells.					
32526222	1	47	theme	loading	521:527	arg1	pathways					485:492	pathways	485:492	pathways of EV biogenesis and cargo loading	485:527	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	48	theme	proteomic	220:228	arg1	studies					230:236	proteomic studies	220:236	proteomic studies of EVs from representatives of nematodes, cestodes and trematodes	220:302	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	49	from	studies	230:236	arg1	representatives					250:264	representatives	250:264	representatives of nematodes, cestodes and trematodes	250:302	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	50	theme	heat-shock	437:446	arg1	proteins					448:455	heat-shock proteins	437:455	heat-shock proteins	437:455	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	3	51	theme	glycan	864:869	arg1	composition					871:881	glycan composition	864:881	glycan composition	864:881	This opinion article highlights two exceptional studies that identified EVs released by Heligmosomoides polygyrus and Fasciola hepatica which display differential lipid and glycan composition, respectively, when compared with EVs derived from mammalian cells.					
32526222	4	52	theme	glycan	1027:1032	arg1	composition					1034:1044	glycan composition	1027:1044	glycan composition	1027:1044	Furthermore, we discuss the potential implications of helminth EV lipid and glycan composition upon helminth infection and host pathology.					
32526222	3	53	theme	differential	841:852	arg1	lipid					854:858	differential lipid	841:858	differential lipid	841:858	This opinion article highlights two exceptional studies that identified EVs released by Heligmosomoides polygyrus and Fasciola hepatica which display differential lipid and glycan composition, respectively, when compared with EVs derived from mammalian cells.					
32526222	0	54	theme	vesicles	70:77	arg1	glycan					7:12	Unique glycan	0:12	Unique glycan	0:12	Unique glycan and lipid composition of helminth-derived extracellular vesicles may reveal novel roles in host-parasite interactions.					
32526222	0	54	theme	vesicles	70:77	arg1	composition					24:34	lipid composition	18:34	lipid composition	18:34	Unique glycan and lipid composition of helminth-derived extracellular vesicles may reveal novel roles in host-parasite interactions.					
32526222	1	55	theme	mammalian	332:340	arg1	proteins					345:352	mammalian EV proteins	332:352	mammalian EV proteins including components of the endosomal sorting complexes required for transport	332:431	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	55	theme	mammalian	332:340	arg1	components					364:373	components	364:373	components of the endosomal sorting complexes required for transport	364:431	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	5	56	dep	composition	1129:1139	arg1	the					1118:1120	the	1118:1120	the	1118:1120	Future studies, focusing on the unique composition and functional properties of helminth EVs, may prove crucial to the understanding of host-parasite communication.					
32526222	1	57	theme	helminth-derived	155:170	arg1	EVs					196:198	EVs	196:198	EVs	196:198	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	1	57	theme	helminth-derived	155:170	arg1	vesicles					186:193	helminth-derived extracellular vesicles	155:193	helminth-derived extracellular vesicles (EVs)	155:199	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
32526222	3	58	theme	mammalian	934:942	arg1	cells					944:948	mammalian cells	934:948	mammalian cells	934:948	This opinion article highlights two exceptional studies that identified EVs released by Heligmosomoides polygyrus and Fasciola hepatica which display differential lipid and glycan composition, respectively, when compared with EVs derived from mammalian cells.					
32526222	1	59	theme	EVs	241:243	arg1	studies					230:236	proteomic studies	220:236	proteomic studies of EVs from representatives of nematodes, cestodes and trematodes	220:302	Although the study of helminth-derived extracellular vesicles (EVs) is in its infancy, proteomic studies of EVs from representatives of nematodes, cestodes and trematodes have identified homologs of mammalian EV proteins including components of the endosomal sorting complexes required for transport and heat-shock proteins, suggesting conservation of pathways of EV biogenesis and cargo loading between helminths and their hosts.					
31952476	7	0	from	process	1097:1103	arg1	UTCs					1129:1132	UTCs	1129:1132	UTCs	1129:1132	The process of developing TGCs from UTCs was insufficiently understood.					
31952476	14	1	theme	reduced	2362:2368	arg1	anchoring					2370:2378	reduced anchoring	2362:2378	reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium	2362:2523	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	2	2	theme	branched	375:382	arg1	villi					400:404	widely branched fetal chorionic villi	368:404	widely branched fetal chorionic villi	368:404	Here, widely branched fetal chorionic villi interdigitate with corresponding maternal caruncular crypts.					
31952476	1	3	theme	bovine	274:279	arg1	placenta					281:288	the bovine placenta	270:288	the bovine placenta	270:288	BACKGROUND In the bovine placenta, intimate fetomaternal contacts are restricted to discrete placentomes.					
31952476	8	4	theme	expressed	1210:1218	arg1	DEGs					1227:1230	DEGs	1227:1230	DEGs	1227:1230	Therefore, we aimed to detect differentially expressed genes (DEGs) between UTCs and TGCs and identify molecular functions and biological processes regulated by DEGs.					
31952476	8	4	theme	expressed	1210:1218	arg1	genes					1220:1224	differentially expressed genes	1195:1224	differentially expressed genes (DEGs) between UTCs and TGCs	1195:1253	Therefore, we aimed to detect differentially expressed genes (DEGs) between UTCs and TGCs and identify molecular functions and biological processes regulated by DEGs.					
31952476	3	5	theme	uninucleate	559:569	arg1	UTCs					590:593	UTCs	590:593	UTCs	590:593	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	3	5	theme	uninucleate	559:569	arg1	cells					583:587	uninucleate trophoblast cells	559:587	approximately 80% uninucleate trophoblast cells (UTCs)	541:594	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	6	6	theme	UTC/TGC	942:948	arg1	ratio					950:954	The UTC/TGC ratio	938:954	The UTC/TGC ratio in the trophoblast	938:973	The UTC/TGC ratio in the trophoblast remains almost constant because approximately as many new TGCs are produced from UTCs as are consumed by the fusions.					
31952476	13	7	theme	UTCs	2178:2181	arg1	differentiation					2159:2173	The differentiation	2155:2173	The differentiation of UTCs into TGCs	2155:2191	CONCLUSION The differentiation of UTCs into TGCs particularly regulates genes that enable trophoblast cells to interact with their environment.					
31952476	0	8	theme	extracellular	208:220	arg1	matrix					222:227	the extracellular matrix	204:227	the extracellular matrix	204:227	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	12	9	gly	sialylated	1912:1921	arg1	O-glycans					1935:1943	sialylated short-chain O-glycans	1912:1943	sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans)	1912:1974	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	12	9	gly	sialylated	1912:1921	arg1	antigen					1949:1955	Tn antigen	1946:1955	Tn antigen	1946:1955	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	9	10	theme	change	1473:1478	arg1	values					1480:1485	fold change values	1468:1485	fold change values < - 1.5 or > 1.5	1468:1502	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	9	11	from	patterns	1368:1375	arg1	isolates					1407:1414	virtually pure UTC and TGC isolates	1380:1414	virtually pure UTC and TGC isolates	1380:1414	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	8	12	theme	biological	1292:1301	arg1	processes					1303:1311	biological processes	1292:1311	biological processes	1292:1311	Therefore, we aimed to detect differentially expressed genes (DEGs) between UTCs and TGCs and identify molecular functions and biological processes regulated by DEGs.					
31952476	11	13	theme	cellular	1768:1775	arg1	receptors					1777:1785	cellular receptors	1768:1785	cellular receptors	1768:1785	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	3	14	theme	giant	626:630	arg1	TGCs					639:642	TGCs	639:642	TGCs	639:642	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	3	14	theme	giant	626:630	arg1	cells					632:636	binuclear trophoblast giant cells	604:636	20% binuclear trophoblast giant cells (TGCs)	600:643	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	12	15	theme	core	1958:1961	arg1	O-glycans					1965:1973	core 1 O-glycans	1958:1973	core 1 O-glycans	1958:1973	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	6	16	theme	new	1029:1031	arg1	TGCs					1033:1036	approximately as many new TGCs	1007:1036	approximately as many new TGCs	1007:1036	The UTC/TGC ratio in the trophoblast remains almost constant because approximately as many new TGCs are produced from UTCs as are consumed by the fusions.					
31952476	12	17	theme	complex	2061:2067	arg1	O-glycans					2101:2109	complex (i.e., branched and long-chain) O-glycans	2061:2109	complex (i.e., branched and long-chain) O-glycans	2061:2109	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	7	18	theme	TGCs	1119:1122	arg1	process					1097:1103	The process	1093:1103	The process of developing TGCs from UTCs	1093:1132	The process of developing TGCs from UTCs was insufficiently understood.					
31952476	3	19	theme	binuclear	604:612	arg1	TGCs					639:642	TGCs	639:642	TGCs	639:642	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	3	19	theme	binuclear	604:612	arg1	cells					632:636	binuclear trophoblast giant cells	604:636	20% binuclear trophoblast giant cells (TGCs)	600:643	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	15	20	theme	O-glycans	2586:2594	arg1	expression					2549:2558	increased expression	2539:2558	increased expression of sialylated short chain O-glycans by TGCs	2539:2602	Furthermore, increased expression of sialylated short chain O-glycans by TGCs could facilitate the modulation of maternal immune tolerance.					
31952476	12	21	theme	short-chain	1923:1933	arg1	O-glycans					1935:1943	sialylated short-chain O-glycans	1912:1943	sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans)	1912:1974	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	12	21	theme	short-chain	1923:1933	arg1	antigen					1949:1955	Tn antigen	1946:1955	Tn antigen	1946:1955	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	9	22	theme	pure	1390:1393	arg1	isolates					1407:1414	virtually pure UTC and TGC isolates	1380:1414	virtually pure UTC and TGC isolates	1380:1414	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	13	23	theme	trophoblast	2234:2244	arg1	cells					2246:2250	trophoblast cells	2234:2250	trophoblast cells	2234:2250	CONCLUSION The differentiation of UTCs into TGCs particularly regulates genes that enable trophoblast cells to interact with their environment.					
31952476	4	24	theme	short-lived	771:781	arg1	cells					803:807	short-lived fetomaternal hybrid cells	771:807	short-lived fetomaternal hybrid cells	771:807	The weakly invasive TGCs migrate toward the caruncle epithelium and eventually fuse with individual epithelial cells to form short-lived fetomaternal hybrid cells.					
31952476	12	25	theme	O-glycan	2006:2013	arg1	structures					2020:2029	other O-glycan core structures	2000:2029	other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans	2000:2109	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	11	26	with	matrix	1729:1734	arg1	receptors					1777:1785	cellular receptors	1768:1785	cellular receptors	1768:1785	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	0	27	from	differentiation	17:31	arg1	placenta					47:54	the bovine placenta	36:54	the bovine placenta	36:54	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	4	28	theme	epithelial	746:755	arg1	cells					757:761	individual epithelial cells	735:761	individual epithelial cells	735:761	The weakly invasive TGCs migrate toward the caruncle epithelium and eventually fuse with individual epithelial cells to form short-lived fetomaternal hybrid cells.					
31952476	15	29	gly	sialylated	2563:2572	arg1	O-glycans					2586:2594	sialylated short chain O-glycans	2563:2594	sialylated short chain O-glycans	2563:2594	Furthermore, increased expression of sialylated short chain O-glycans by TGCs could facilitate the modulation of maternal immune tolerance.					
31952476	0	30	theme	uninucleate	96:106	arg1	cells					120:124	uninucleate trophoblast cells	96:124	uninucleate trophoblast cells	96:124	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	9	31	theme	gene	1352:1355	arg1	patterns					1368:1375	gene expression patterns	1352:1375	gene expression patterns in virtually pure UTC and TGC isolates	1352:1414	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	2	32	theme	maternal	439:446	arg1	crypts					459:464	corresponding maternal caruncular crypts	425:464	corresponding maternal caruncular crypts	425:464	Here, widely branched fetal chorionic villi interdigitate with corresponding maternal caruncular crypts.					
31952476	15	33	theme	immune	2648:2653	arg1	tolerance					2655:2663	maternal immune tolerance	2639:2663	maternal immune tolerance	2639:2663	Furthermore, increased expression of sialylated short chain O-glycans by TGCs could facilitate the modulation of maternal immune tolerance.					
31952476	11	34	with	interactions	1750:1761	arg1	receptors					1777:1785	cellular receptors	1768:1785	cellular receptors	1768:1785	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	3	35	dep	%	557:557	arg1	UTCs					590:593	UTCs	590:593	UTCs	590:593	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	3	35	dep	%	557:557	arg1	cells					583:587	uninucleate trophoblast cells	559:587	approximately 80% uninucleate trophoblast cells (UTCs)	541:594	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	13	36	dep	CONCLUSION	2144:2153	arg1	regulates					2206:2214	regulates	2206:2214	regulates genes that enable trophoblast cells to interact with their environment	2206:2285	CONCLUSION The differentiation of UTCs into TGCs particularly regulates genes that enable trophoblast cells to interact with their environment.					
31952476	12	37	dep	antigen	1949:1955	arg1	O-glycans					1965:1973	core 1 O-glycans	1958:1973	core 1 O-glycans	1958:1973	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	4	38	theme	hybrid	796:801	arg1	cells					803:807	short-lived fetomaternal hybrid cells	771:807	short-lived fetomaternal hybrid cells	771:807	The weakly invasive TGCs migrate toward the caruncle epithelium and eventually fuse with individual epithelial cells to form short-lived fetomaternal hybrid cells.					
31952476	3	39	theme	fetal	471:475	arg1	epithelium					489:498	The fetal trophoblast epithelium	467:498	The fetal trophoblast epithelium covering the chorionic villi	467:527	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	0	40	theme	Trophoblast	0:10	arg1	differentiation					17:31	Trophoblast cell differentiation	0:31	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells	0:152	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	1	41	theme	intimate	291:298	arg1	contacts					313:320	intimate fetomaternal contacts	291:320	intimate fetomaternal contacts	291:320	BACKGROUND In the bovine placenta, intimate fetomaternal contacts are restricted to discrete placentomes.					
31952476	12	42	theme	O-glycan	1870:1877	arg1	biosynthesis					1879:1890	O-glycan biosynthesis	1870:1890	O-glycan biosynthesis in TGCs	1870:1898	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	7	43	from	UTCs	1129:1132	arg1	process					1097:1103	The process	1093:1103	The process of developing TGCs from UTCs	1093:1132	The process of developing TGCs from UTCs was insufficiently understood.					
31952476	7	43	from	UTCs	1129:1132	arg1	TGCs					1119:1122	developing TGCs	1108:1122	developing TGCs from UTCs	1108:1132	The process of developing TGCs from UTCs was insufficiently understood.					
31952476	9	44	theme	UTC	1395:1397	arg1	isolates					1407:1414	virtually pure UTC and TGC isolates	1380:1414	virtually pure UTC and TGC isolates	1380:1414	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	9	45	dep	RESULTS	1332:1338	arg1	detected					1438:1445	detected	1438:1445	detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5)	1438:1503	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	9	45	dep	RESULTS	1332:1338	arg1	analyzed					1343:1350	analyzed	1343:1350	analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays	1343:1432	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	9	46	theme	TGC	1403:1405	arg1	isolates					1407:1414	virtually pure UTC and TGC isolates	1380:1414	virtually pure UTC and TGC isolates	1380:1414	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	14	47	theme	maternal	2504:2511	arg1	endometrium					2513:2523	the maternal endometrium	2500:2523	the maternal endometrium	2500:2523	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	5	48	theme	origin	842:847	arg1	molecules					823:831	molecules	823:831	molecules of fetal origin	823:847	In this way, molecules of fetal origin are transported across the placental barrier and released into the maternal compartment.					
31952476	1	49	theme	discrete	340:347	arg1	placentomes					349:359	discrete placentomes	340:359	discrete placentomes	340:359	BACKGROUND In the bovine placenta, intimate fetomaternal contacts are restricted to discrete placentomes.					
31952476	12	50	theme	O-glycans	2101:2109	arg1	formation					2048:2056	the formation	2044:2056	the formation of complex (i.e., branched and long-chain) O-glycans	2044:2109	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	11	51	theme	signal	1807:1812	arg1	interactions					1750:1761	its interactions	1746:1761	its interactions with cellular receptors	1746:1785	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	11	51	theme	signal	1807:1812	arg1	transduction					1814:1825	signal transduction	1807:1825	signal transduction	1807:1825	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	15	52	theme	chain	2580:2584	arg1	O-glycans					2586:2594	sialylated short chain O-glycans	2563:2594	sialylated short chain O-glycans	2563:2594	Furthermore, increased expression of sialylated short chain O-glycans by TGCs could facilitate the modulation of maternal immune tolerance.					
31952476	14	53	theme	invasive	2474:2481	arg1	interaction					2483:2493	their weakly invasive interaction	2461:2493	their weakly invasive interaction with the maternal endometrium	2461:2523	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	0	54	dep	differentiation	17:31	arg1	genes					82:86	differentially expressed genes	57:86	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells	0:152	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	14	55	from	differences	2300:2310	arg1	composition					2341:2351	ECM composition	2337:2351	ECM composition	2337:2351	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	3	56	theme	chorionic	513:521	arg1	villi					523:527	the chorionic villi	509:527	the chorionic villi	509:527	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	14	57	theme	TGCs	2383:2386	arg1	anchoring					2370:2378	reduced anchoring	2362:2378	reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium	2362:2523	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	4	58	theme	caruncle	690:697	arg1	epithelium					699:708	the caruncle epithelium	686:708	the caruncle epithelium	686:708	The weakly invasive TGCs migrate toward the caruncle epithelium and eventually fuse with individual epithelial cells to form short-lived fetomaternal hybrid cells.					
31952476	11	59	theme	Gene	1591:1594	arg1	Ontology					1596:1603	Gene Ontology	1591:1603	Gene Ontology (GO) analyses	1591:1617	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	2	60	theme	fetal	384:388	arg1	villi					400:404	widely branched fetal chorionic villi	368:404	widely branched fetal chorionic villi	368:404	Here, widely branched fetal chorionic villi interdigitate with corresponding maternal caruncular crypts.					
31952476	1	61	from	restricted	326:335	arg1	placenta					281:288	the bovine placenta	270:288	the bovine placenta	270:288	BACKGROUND In the bovine placenta, intimate fetomaternal contacts are restricted to discrete placentomes.					
31952476	12	62	theme	structures	2020:2029	arg1	synthesis					1987:1995	the synthesis	1983:1995	the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans	1983:2109	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	6	63	from	ratio	950:954	arg1	trophoblast					963:973	the trophoblast	959:973	the trophoblast	959:973	The UTC/TGC ratio in the trophoblast remains almost constant because approximately as many new TGCs are produced from UTCs as are consumed by the fusions.					
31952476	0	64	theme	matrix	222:227	arg1	remodeling					190:199	remodeling	190:199	remodeling	190:199	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	0	64	theme	matrix	222:227	arg1	composition					174:184	composition	174:184	composition	174:184	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	14	65	theme	ECM	2337:2339	arg1	composition					2341:2351	ECM composition	2337:2351	ECM composition	2337:2351	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	9	66	theme	fold	1468:1471	arg1	values					1480:1485	fold change values	1468:1485	fold change values < - 1.5 or > 1.5	1468:1502	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	8	67	theme	molecular	1268:1276	arg1	functions					1278:1286	molecular functions	1268:1286	molecular functions	1268:1286	Therefore, we aimed to detect differentially expressed genes (DEGs) between UTCs and TGCs and identify molecular functions and biological processes regulated by DEGs.					
31952476	11	68	theme	molecular	1633:1641	arg1	functions					1643:1651	molecular functions	1633:1651	molecular functions	1633:1651	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	0	69	theme	O-glycan	233:240	arg1	biosynthesis					242:253	O-glycan biosynthesis	233:253	O-glycan biosynthesis	233:253	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	3	70	theme	trophoblast	614:624	arg1	TGCs					639:642	TGCs	639:642	TGCs	639:642	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	3	70	theme	trophoblast	614:624	arg1	cells					632:636	binuclear trophoblast giant cells	604:636	20% binuclear trophoblast giant cells (TGCs)	600:643	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	5	71	theme	maternal	916:923	arg1	compartment					925:935	the maternal compartment	912:935	the maternal compartment	912:935	In this way, molecules of fetal origin are transported across the placental barrier and released into the maternal compartment.					
31952476	12	72	theme	Tn	1946:1947	arg1	O-glycans					1935:1943	sialylated short-chain O-glycans	1912:1943	sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans)	1912:1974	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	12	72	theme	Tn	1946:1947	arg1	antigen					1949:1955	Tn antigen	1946:1955	Tn antigen	1946:1955	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	0	73	theme	giant	142:146	arg1	cells					148:152	trophoblast giant cells	130:152	trophoblast giant cells	130:152	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	5	74	theme	placental	876:884	arg1	barrier					886:892	the placental barrier	872:892	the placental barrier	872:892	In this way, molecules of fetal origin are transported across the placental barrier and released into the maternal compartment.					
31952476	6	75	theme	many	1024:1027	arg1	TGCs					1033:1036	approximately as many new TGCs	1007:1036	approximately as many new TGCs	1007:1036	The UTC/TGC ratio in the trophoblast remains almost constant because approximately as many new TGCs are produced from UTCs as are consumed by the fusions.					
31952476	14	76	theme	surrounding	2395:2405	arg1	matrix					2407:2412	the surrounding matrix	2391:2412	the surrounding matrix	2391:2412	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	1	77	dep	BACKGROUND	256:265	arg1	restricted					326:335	restricted	326:335	restricted	326:335	BACKGROUND In the bovine placenta, intimate fetomaternal contacts are restricted to discrete placentomes.					
31952476	12	78	theme	core	2015:2018	arg1	structures					2020:2029	other O-glycan core structures	2000:2029	other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans	2000:2109	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	11	79	dep	Ontology	1596:1603	arg1	GO					1606:1607	GO	1606:1607	GO	1606:1607	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	12	80	theme	sialylated	1912:1921	arg1	O-glycans					1935:1943	sialylated short-chain O-glycans	1912:1943	sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans)	1912:1974	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	12	80	theme	sialylated	1912:1921	arg1	antigen					1949:1955	Tn antigen	1946:1955	Tn antigen	1946:1955	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	15	81	theme	tolerance	2655:2663	arg1	modulation					2625:2634	the modulation	2621:2634	the modulation of maternal immune tolerance	2621:2663	Furthermore, increased expression of sialylated short chain O-glycans by TGCs could facilitate the modulation of maternal immune tolerance.					
31952476	0	82	dep	composition	174:184	arg1	the					170:172	the	170:172	the	170:172	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	1	83	from	placenta	281:288	arg1	restricted					326:335	restricted	326:335	restricted	326:335	BACKGROUND In the bovine placenta, intimate fetomaternal contacts are restricted to discrete placentomes.					
31952476	7	84	theme	developing	1108:1117	arg1	TGCs					1119:1122	developing TGCs	1108:1122	developing TGCs from UTCs	1108:1132	The process of developing TGCs from UTCs was insufficiently understood.					
31952476	9	85	dep	p < 0.05	1458:1465	arg1	values					1480:1485	fold change values	1468:1485	fold change values < - 1.5 or > 1.5	1468:1502	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	14	86	from	matrix	2407:2412	arg1	anchoring					2370:2378	reduced anchoring	2362:2378	reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium	2362:2523	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	11	87	theme	cell	1788:1791	arg1	interactions					1750:1761	its interactions	1746:1761	its interactions with cellular receptors	1746:1785	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	11	87	theme	cell	1788:1791	arg1	migration					1793:1801	cell migration	1788:1801	cell migration	1788:1801	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	9	88	dep	DEGs	1452:1455	arg1	p < 0.05					1458:1465	p < 0.05	1458:1465	p < 0.05	1458:1465	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	14	89	from	anchoring	2370:2378	arg1	matrix					2407:2412	the surrounding matrix	2391:2412	the surrounding matrix	2391:2412	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	14	90	theme	Significant	2288:2298	arg1	differences					2300:2310	Significant differences	2288:2310	Significant differences between UTCs and TGCs in ECM composition	2288:2351	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	0	91	theme	expressed	72:80	arg1	genes					82:86	differentially expressed genes	57:86	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells	0:152	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	3	92	dep	%	602:602	arg1	TGCs					639:642	TGCs	639:642	TGCs	639:642	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	3	92	dep	%	602:602	arg1	cells					632:636	binuclear trophoblast giant cells	604:636	20% binuclear trophoblast giant cells (TGCs)	600:643	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	14	93	from	TGCs	2383:2386	arg1	matrix					2407:2412	the surrounding matrix	2391:2412	the surrounding matrix	2391:2412	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	12	94	theme	other	2000:2004	arg1	structures					2020:2029	other O-glycan core structures	2000:2029	other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans	2000:2109	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	11	95	theme	extracellular	1715:1727	arg1	matrix					1729:1734	the extracellular matrix	1711:1734	the extracellular matrix (ECM)	1711:1740	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	11	95	theme	extracellular	1715:1727	arg1	ECM					1737:1739	ECM	1737:1739	ECM	1737:1739	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	12	96	from	biosynthesis	1879:1890	arg1	TGCs					1895:1898	TGCs	1895:1898	TGCs	1895:1898	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	0	97	theme	trophoblast	130:140	arg1	cells					148:152	trophoblast giant cells	130:152	trophoblast giant cells	130:152	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	4	98	theme	individual	735:744	arg1	cells					757:761	individual epithelial cells	735:761	individual epithelial cells	735:761	The weakly invasive TGCs migrate toward the caruncle epithelium and eventually fuse with individual epithelial cells to form short-lived fetomaternal hybrid cells.					
31952476	0	99	theme	trophoblast	108:118	arg1	cells					120:124	uninucleate trophoblast cells	96:124	uninucleate trophoblast cells	96:124	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	0	100	theme	biosynthesis	242:253	arg1	remodeling					190:199	remodeling	190:199	remodeling	190:199	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	0	100	theme	biosynthesis	242:253	arg1	composition					174:184	composition	174:184	composition	174:184	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	14	101	with	interaction	2483:2493	arg1	endometrium					2513:2523	the maternal endometrium	2500:2523	the maternal endometrium	2500:2523	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	15	102	theme	maternal	2639:2646	arg1	tolerance					2655:2663	maternal immune tolerance	2639:2663	maternal immune tolerance	2639:2663	Furthermore, increased expression of sialylated short chain O-glycans by TGCs could facilitate the modulation of maternal immune tolerance.					
31952476	9	103	theme	expression	1357:1366	arg1	patterns					1368:1375	gene expression patterns	1352:1375	gene expression patterns in virtually pure UTC and TGC isolates	1352:1414	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	2	104	theme	corresponding	425:437	arg1	crypts					459:464	corresponding maternal caruncular crypts	425:464	corresponding maternal caruncular crypts	425:464	Here, widely branched fetal chorionic villi interdigitate with corresponding maternal caruncular crypts.					
31952476	0	105	theme	cell	12:15	arg1	differentiation					17:31	Trophoblast cell differentiation	0:31	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells	0:152	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	14	106	with	TGCs	2383:2386	arg1	endometrium					2513:2523	the maternal endometrium	2500:2523	the maternal endometrium	2500:2523	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	4	107	theme	fetomaternal	783:794	arg1	cells					803:807	short-lived fetomaternal hybrid cells	771:807	short-lived fetomaternal hybrid cells	771:807	The weakly invasive TGCs migrate toward the caruncle epithelium and eventually fuse with individual epithelial cells to form short-lived fetomaternal hybrid cells.					
31952476	3	108	theme	trophoblast	477:487	arg1	epithelium					489:498	The fetal trophoblast epithelium	467:498	The fetal trophoblast epithelium covering the chorionic villi	467:527	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	1	109	theme	fetomaternal	300:311	arg1	contacts					313:320	intimate fetomaternal contacts	291:320	intimate fetomaternal contacts	291:320	BACKGROUND In the bovine placenta, intimate fetomaternal contacts are restricted to discrete placentomes.					
31952476	0	110	theme	bovine	40:45	arg1	placenta					47:54	the bovine placenta	36:54	the bovine placenta	36:54	Trophoblast cell differentiation in the bovine placenta: differentially expressed genes between uninucleate trophoblast cells and trophoblast giant cells are involved in the composition and remodeling of the extracellular matrix and O-glycan biosynthesis.					
31952476	15	111	theme	sialylated	2563:2572	arg1	O-glycans					2586:2594	sialylated short chain O-glycans	2563:2594	sialylated short chain O-glycans	2563:2594	Furthermore, increased expression of sialylated short chain O-glycans by TGCs could facilitate the modulation of maternal immune tolerance.					
31952476	11	112	theme	biological	1657:1666	arg1	processes					1668:1676	biological processes	1657:1676	biological processes	1657:1676	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	2	113	theme	caruncular	448:457	arg1	crypts					459:464	corresponding maternal caruncular crypts	425:464	corresponding maternal caruncular crypts	425:464	Here, widely branched fetal chorionic villi interdigitate with corresponding maternal caruncular crypts.					
31952476	5	114	theme	fetal	836:840	arg1	origin					842:847	fetal origin	836:847	fetal origin	836:847	In this way, molecules of fetal origin are transported across the placental barrier and released into the maternal compartment.					
31952476	12	115	dep	complex	2061:2067	arg1	branched					2076:2083	branched	2076:2083	branched	2076:2083	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	12	115	dep	complex	2061:2067	arg1	long-chain					2089:2098	long-chain	2089:2098	long-chain	2089:2098	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	3	116	theme	trophoblast	571:581	arg1	UTCs					590:593	UTCs	590:593	UTCs	590:593	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	3	116	theme	trophoblast	571:581	arg1	cells					583:587	uninucleate trophoblast cells	559:587	approximately 80% uninucleate trophoblast cells (UTCs)	541:594	The fetal trophoblast epithelium covering the chorionic villi consists of approximately 80% uninucleate trophoblast cells (UTCs) and 20% binuclear trophoblast giant cells (TGCs).					
31952476	15	117	theme	short	2574:2578	arg1	O-glycans					2586:2594	sialylated short chain O-glycans	2563:2594	sialylated short chain O-glycans	2563:2594	Furthermore, increased expression of sialylated short chain O-glycans by TGCs could facilitate the modulation of maternal immune tolerance.					
31952476	9	118	theme	gene	1422:1425	arg1	arrays					1427:1432	gene arrays	1422:1432	gene arrays	1422:1432	RESULTS We analyzed gene expression patterns in virtually pure UTC and TGC isolates using gene arrays and detected 3193 DEGs (p < 0.05; fold change values < - 1.5 or > 1.5).					
31952476	14	119	theme	interaction	2483:2493	arg1	anchoring					2370:2378	reduced anchoring	2362:2378	reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium	2362:2523	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	14	120	from	interaction	2483:2493	arg1	matrix					2407:2412	the surrounding matrix	2391:2412	the surrounding matrix	2391:2412	Significant differences between UTCs and TGCs in ECM composition indicate reduced anchoring of TGCs in the surrounding matrix, which might contribute to their migration and their weakly invasive interaction with the maternal endometrium.					
31952476	11	121	theme	Ontology	1596:1603	arg1	analyses					1610:1617	Gene Ontology (GO) analyses	1591:1617	Gene Ontology (GO) analyses	1591:1617	Gene Ontology (GO) analyses revealed that molecular functions and biological processes regulated by DEGs are related to the extracellular matrix (ECM) and its interactions with cellular receptors, cell migration and signal transduction.					
31952476	12	122	dep	branched	2076:2083	arg1	i.e.					2070:2073	i.e.	2070:2073	i.e.	2070:2073	Furthermore, there was some evidence that O-glycan biosynthesis in TGCs may produce sialylated short-chain O-glycans (Tn antigen, core 1 O-glycans), while the synthesis of other O-glycan core structures required for the formation of complex (i.e., branched and long-chain) O-glycans appears to be decreased in TGCs.					
31952476	4	123	theme	invasive	657:664	arg1	TGCs					666:669	The weakly invasive TGCs	646:669	The weakly invasive TGCs	646:669	The weakly invasive TGCs migrate toward the caruncle epithelium and eventually fuse with individual epithelial cells to form short-lived fetomaternal hybrid cells.					
31952476	2	124	theme	chorionic	390:398	arg1	villi					400:404	widely branched fetal chorionic villi	368:404	widely branched fetal chorionic villi	368:404	Here, widely branched fetal chorionic villi interdigitate with corresponding maternal caruncular crypts.					
31952476	15	125	theme	increased	2539:2547	arg1	expression					2549:2558	increased expression	2539:2558	increased expression of sialylated short chain O-glycans by TGCs	2539:2602	Furthermore, increased expression of sialylated short chain O-glycans by TGCs could facilitate the modulation of maternal immune tolerance.					
34662214	9	0	theme	macrophage	1998:2007	arg1	trafficking					2009:2019	macrophage trafficking	1998:2019	macrophage trafficking	1998:2019	Together, these findings strongly suggest that while neuraminidase-mediated N-glycan modification impaired both macrophage phagocytosis and galvanotaxis, yet to be defined mechanisms other than NanH may play a more important role in bioelectrical control of macrophage trafficking, which potentially triggers dissemination.					
34662214	8	1	theme	wild	1579:1582	arg1	type					1584:1587	wild type Salmonella	1579:1598	wild type Salmonella	1579:1598	However, compared to wild type Salmonella, infection with the nanH mutant only marginally reduced the cathode-oriented macrophage galvonotaxis, without canceling direction reversal.					
34662214	4	2	theme	bacterial	782:790	arg1	neuraminidase					792:804	a bacterial neuraminidase	780:804	a bacterial neuraminidase	780:804	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	4	3	theme	surface	670:676	arg1	composition					685:695	host cell surface glycan composition	660:695	host cell surface glycan composition	660:695	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	0	4	theme	Independent	102:112	arg1	Galvanotaxis					89:100	Macrophage Galvanotaxis	78:100	Macrophage Galvanotaxis Independent of NanH	78:120	Surface Glycans Regulate Salmonella Infection-Dependent Directional Switch in Macrophage Galvanotaxis Independent of NanH.					
34662214	2	5	theme	bioelectrical	402:414	arg1	mechanism					416:424	a bioelectrical mechanism	400:424	a bioelectrical mechanism to initiate life-threatening disseminations	400:468	Phagocytosis of bacteria reverses the direction of macrophage galvanotaxis, implicating a bioelectrical mechanism to initiate life-threatening disseminations.					
34662214	4	6	theme	cell	665:668	arg1	composition					685:695	host cell surface glycan composition	660:695	host cell surface glycan composition	660:695	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	9	7	theme	macrophage	1852:1861	arg1	phagocytosis					1863:1874	macrophage phagocytosis	1852:1874	macrophage phagocytosis	1852:1874	Together, these findings strongly suggest that while neuraminidase-mediated N-glycan modification impaired both macrophage phagocytosis and galvanotaxis, yet to be defined mechanisms other than NanH may play a more important role in bioelectrical control of macrophage trafficking, which potentially triggers dissemination.					
34662214	6	8	theme	human	1260:1264	arg1	model					1300:1304	a human colonic epithelial cell infection model	1258:1304	a human colonic epithelial cell infection model	1258:1304	The Salmonella nanH gene, encoding a putative neuraminidase, is required for invasion and internalization in a human colonic epithelial cell infection model.					
34662214	0	9	theme	NanH	117:120	arg1	Independent					102:112	Independent	102:112	Independent	102:112	Surface Glycans Regulate Salmonella Infection-Dependent Directional Switch in Macrophage Galvanotaxis Independent of NanH.					
34662214	8	10	theme	macrophage	1677:1686	arg1	galvonotaxis					1688:1699	the cathode-oriented macrophage galvonotaxis	1656:1699	the cathode-oriented macrophage galvonotaxis	1656:1699	However, compared to wild type Salmonella, infection with the nanH mutant only marginally reduced the cathode-oriented macrophage galvonotaxis, without canceling direction reversal.					
34662214	7	11	theme	direction	1384:1392	arg1	reversal					1394:1401	the Salmonella infection-dependent direction reversal	1349:1401	the Salmonella infection-dependent direction reversal	1349:1401	To determine whether NanH is required for the Salmonella infection-dependent direction reversal, we constructed and characterized a nanH deletion mutant and found that NanH is partially required for Salmonella infection in primary murine macrophages.					
34662214	2	12	theme	bacteria	328:335	arg1	Phagocytosis					312:323	Phagocytosis	312:323	Phagocytosis of bacteria	312:335	Phagocytosis of bacteria reverses the direction of macrophage galvanotaxis, implicating a bioelectrical mechanism to initiate life-threatening disseminations.					
34662214	9	13	theme	important	1955:1963	arg1	role					1965:1968	a more important role	1948:1968	a more important role	1948:1968	Together, these findings strongly suggest that while neuraminidase-mediated N-glycan modification impaired both macrophage phagocytosis and galvanotaxis, yet to be defined mechanisms other than NanH may play a more important role in bioelectrical control of macrophage trafficking, which potentially triggers dissemination.					
34662214	2	14	theme	galvanotaxis	374:385	arg1	direction					350:358	the direction	346:358	the direction of macrophage galvanotaxis	346:385	Phagocytosis of bacteria reverses the direction of macrophage galvanotaxis, implicating a bioelectrical mechanism to initiate life-threatening disseminations.					
34662214	9	15	theme	bioelectrical	1973:1985	arg1	control					1987:1993	bioelectrical control	1973:1993	bioelectrical control	1973:1993	Together, these findings strongly suggest that while neuraminidase-mediated N-glycan modification impaired both macrophage phagocytosis and galvanotaxis, yet to be defined mechanisms other than NanH may play a more important role in bioelectrical control of macrophage trafficking, which potentially triggers dissemination.					
34662214	8	16	theme	cathode-oriented	1660:1675	arg1	galvonotaxis					1688:1699	the cathode-oriented macrophage galvonotaxis	1656:1699	the cathode-oriented macrophage galvonotaxis	1656:1699	However, compared to wild type Salmonella, infection with the nanH mutant only marginally reduced the cathode-oriented macrophage galvonotaxis, without canceling direction reversal.					
34662214	5	17	theme	neuraminidase-associated	974:997	arg1	shift					1013:1017	the bacterial neuraminidase-associated compositional shift	960:1017	the bacterial neuraminidase-associated compositional shift	960:1017	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	1	18	theme	infection-generated	193:211	arg1	field					222:226	an infection-generated electric field	190:226	an infection-generated electric field that can drive directional migration of macrophages, a process called galvanotaxis	190:309	Salmonella invades and disrupts gut epithelium integrity, creating an infection-generated electric field that can drive directional migration of macrophages, a process called galvanotaxis.					
34662214	4	19	theme	glycan	678:683	arg1	composition					685:695	host cell surface glycan composition	660:695	host cell surface glycan composition	660:695	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	8	20	with	infection	1601:1609	arg1	mutant					1625:1630	the nanH mutant	1616:1630	the nanH mutant	1616:1630	However, compared to wild type Salmonella, infection with the nanH mutant only marginally reduced the cathode-oriented macrophage galvonotaxis, without canceling direction reversal.					
34662214	6	21	theme	infection	1290:1298	arg1	model					1300:1304	a human colonic epithelial cell infection model	1258:1304	a human colonic epithelial cell infection model	1258:1304	The Salmonella nanH gene, encoding a putative neuraminidase, is required for invasion and internalization in a human colonic epithelial cell infection model.					
34662214	1	22	theme	electric	213:220	arg1	field					222:226	an infection-generated electric field	190:226	an infection-generated electric field that can drive directional migration of macrophages, a process called galvanotaxis	190:309	Salmonella invades and disrupts gut epithelium integrity, creating an infection-generated electric field that can drive directional migration of macrophages, a process called galvanotaxis.					
34662214	5	23	gly	fucosylated	1108:1118	arg1	structures					1137:1146	fucosylated and high mannose structures	1108:1146	fucosylated and high mannose structures	1108:1146	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	6	24	theme	cell	1285:1288	arg1	model					1300:1304	a human colonic epithelial cell infection model	1258:1304	a human colonic epithelial cell infection model	1258:1304	The Salmonella nanH gene, encoding a putative neuraminidase, is required for invasion and internalization in a human colonic epithelial cell infection model.					
34662214	2	25	theme	macrophage	363:372	arg1	galvanotaxis					374:385	macrophage galvanotaxis	363:385	macrophage galvanotaxis	363:385	Phagocytosis of bacteria reverses the direction of macrophage galvanotaxis, implicating a bioelectrical mechanism to initiate life-threatening disseminations.					
34662214	6	26	theme	putative	1186:1193	arg1	neuraminidase					1195:1207	a putative neuraminidase	1184:1207	a putative neuraminidase	1184:1207	The Salmonella nanH gene, encoding a putative neuraminidase, is required for invasion and internalization in a human colonic epithelial cell infection model.					
34662214	4	27	theme	sialic	762:767	arg1	acids					769:773	surface-exposed sialic acids	746:773	surface-exposed sialic acids	746:773	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	0	28	theme	Surface	0:6	arg1	Glycans					8:14	Surface Glycans	0:14	Surface Glycans	0:14	Surface Glycans Regulate Salmonella Infection-Dependent Directional Switch in Macrophage Galvanotaxis Independent of NanH.					
34662214	7	29	theme	infection-dependent	1364:1382	arg1	reversal					1394:1401	the Salmonella infection-dependent direction reversal	1349:1401	the Salmonella infection-dependent direction reversal	1349:1401	To determine whether NanH is required for the Salmonella infection-dependent direction reversal, we constructed and characterized a nanH deletion mutant and found that NanH is partially required for Salmonella infection in primary murine macrophages.					
34662214	8	30	theme	nanH	1620:1623	arg1	mutant					1625:1630	the nanH mutant	1616:1630	the nanH mutant	1616:1630	However, compared to wild type Salmonella, infection with the nanH mutant only marginally reduced the cathode-oriented macrophage galvonotaxis, without canceling direction reversal.					
34662214	5	31	theme	bacterial	964:972	arg1	shift					1013:1017	the bacterial neuraminidase-associated compositional shift	960:1017	the bacterial neuraminidase-associated compositional shift	960:1017	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	2	32	theme	life-threatening	438:453	arg1	disseminations					455:468	life-threatening disseminations	438:468	life-threatening disseminations	438:468	Phagocytosis of bacteria reverses the direction of macrophage galvanotaxis, implicating a bioelectrical mechanism to initiate life-threatening disseminations.					
34662214	0	33	theme	Salmonella	25:34	arg1	Switch					68:73	Salmonella Infection-Dependent Directional Switch	25:73	Salmonella Infection-Dependent Directional Switch	25:73	Surface Glycans Regulate Salmonella Infection-Dependent Directional Switch in Macrophage Galvanotaxis Independent of NanH.					
34662214	4	34	theme	macrophages	635:645	arg1	properties					617:626	the electrical properties	602:626	the electrical properties of the macrophages	602:645	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	9	35	theme	trafficking	2009:2019	arg1	control					1987:1993	bioelectrical control	1973:1993	bioelectrical control	1973:1993	Together, these findings strongly suggest that while neuraminidase-mediated N-glycan modification impaired both macrophage phagocytosis and galvanotaxis, yet to be defined mechanisms other than NanH may play a more important role in bioelectrical control of macrophage trafficking, which potentially triggers dissemination.					
34662214	6	36	theme	colonic	1266:1272	arg1	model					1300:1304	a human colonic epithelial cell infection model	1258:1304	a human colonic epithelial cell infection model	1258:1304	The Salmonella nanH gene, encoding a putative neuraminidase, is required for invasion and internalization in a human colonic epithelial cell infection model.					
34662214	7	37	from	infection	1517:1525	arg1	macrophages					1545:1555	primary murine macrophages	1530:1555	primary murine macrophages	1530:1555	To determine whether NanH is required for the Salmonella infection-dependent direction reversal, we constructed and characterized a nanH deletion mutant and found that NanH is partially required for Salmonella infection in primary murine macrophages.					
34662214	5	38	theme	mass	929:932	arg1	cytometry					934:942	nanoLC-chip QTOF mass cytometry	912:942	nanoLC-chip QTOF mass cytometry	912:942	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	5	39	theme	QTOF	924:927	arg1	cytometry					934:942	nanoLC-chip QTOF mass cytometry	912:942	nanoLC-chip QTOF mass cytometry	912:942	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	4	40	theme	host	660:663	arg1	composition					685:695	host cell surface glycan composition	660:695	host cell surface glycan composition	660:695	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	5	41	theme	macrophage	1026:1035	arg1	glycocalyx					1037:1046	the macrophage glycocalyx	1022:1046	the macrophage glycocalyx	1022:1046	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	6	42	theme	epithelial	1274:1283	arg1	model					1300:1304	a human colonic epithelial cell infection model	1258:1304	a human colonic epithelial cell infection model	1258:1304	The Salmonella nanH gene, encoding a putative neuraminidase, is required for invasion and internalization in a human colonic epithelial cell infection model.					
34662214	0	43	theme	Directional	56:66	arg1	Switch					68:73	Salmonella Infection-Dependent Directional Switch	25:73	Salmonella Infection-Dependent Directional Switch	25:73	Surface Glycans Regulate Salmonella Infection-Dependent Directional Switch in Macrophage Galvanotaxis Independent of NanH.					
34662214	8	44	theme	direction	1720:1728	arg1	reversal					1730:1737	direction reversal	1720:1737	direction reversal	1720:1737	However, compared to wild type Salmonella, infection with the nanH mutant only marginally reduced the cathode-oriented macrophage galvonotaxis, without canceling direction reversal.					
34662214	7	45	theme	murine	1538:1543	arg1	macrophages					1545:1555	primary murine macrophages	1530:1555	primary murine macrophages	1530:1555	To determine whether NanH is required for the Salmonella infection-dependent direction reversal, we constructed and characterized a nanH deletion mutant and found that NanH is partially required for Salmonella infection in primary murine macrophages.					
34662214	4	46	theme	surface-exposed	746:760	arg1	acids					769:773	surface-exposed sialic acids	746:773	surface-exposed sialic acids	746:773	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	1	47	theme	directional	243:253	arg1	process					283:289	a process	281:289	a process called galvanotaxis	281:309	Salmonella invades and disrupts gut epithelium integrity, creating an infection-generated electric field that can drive directional migration of macrophages, a process called galvanotaxis.					
34662214	1	47	theme	directional	243:253	arg1	migration					255:263	directional migration	243:263	directional migration of macrophages	243:278	Salmonella invades and disrupts gut epithelium integrity, creating an infection-generated electric field that can drive directional migration of macrophages, a process called galvanotaxis.					
34662214	0	48	theme	Infection-Dependent	36:54	arg1	Switch					68:73	Salmonella Infection-Dependent Directional Switch	25:73	Salmonella Infection-Dependent Directional Switch	25:73	Surface Glycans Regulate Salmonella Infection-Dependent Directional Switch in Macrophage Galvanotaxis Independent of NanH.					
34662214	3	49	theme	direction	493:501	arg1	reversal					503:510	direction reversal	493:510	direction reversal of macrophage galvanotaxis	493:537	The force that drives direction reversal of macrophage galvanotaxis is not understood.					
34662214	5	50	theme	nanoLC-chip	912:922	arg1	cytometry					934:942	nanoLC-chip QTOF mass cytometry	912:942	nanoLC-chip QTOF mass cytometry	912:942	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	9	51	theme	N-glycan	1816:1823	arg1	modification					1825:1836	neuraminidase-mediated N-glycan modification	1793:1836	neuraminidase-mediated N-glycan modification	1793:1836	Together, these findings strongly suggest that while neuraminidase-mediated N-glycan modification impaired both macrophage phagocytosis and galvanotaxis, yet to be defined mechanisms other than NanH may play a more important role in bioelectrical control of macrophage trafficking, which potentially triggers dissemination.					
34662214	6	52	theme	nanH	1164:1167	arg1	gene					1169:1172	The Salmonella nanH gene	1149:1172	The Salmonella nanH gene	1149:1172	The Salmonella nanH gene, encoding a putative neuraminidase, is required for invasion and internalization in a human colonic epithelial cell infection model.					
34662214	4	53	theme	electrical	606:615	arg1	properties					617:626	the electrical properties	602:626	the electrical properties of the macrophages	602:645	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	9	54	theme	other	1923:1927	arg1	mechanisms					1912:1921	mechanisms	1912:1921	mechanisms other than NanH	1912:1937	Together, these findings strongly suggest that while neuraminidase-mediated N-glycan modification impaired both macrophage phagocytosis and galvanotaxis, yet to be defined mechanisms other than NanH may play a more important role in bioelectrical control of macrophage trafficking, which potentially triggers dissemination.					
34662214	3	55	theme	macrophage	515:524	arg1	galvanotaxis					526:537	macrophage galvanotaxis	515:537	macrophage galvanotaxis	515:537	The force that drives direction reversal of macrophage galvanotaxis is not understood.					
34662214	7	56	theme	Salmonella	1506:1515	arg1	infection					1517:1525	Salmonella infection	1506:1525	Salmonella infection in primary murine macrophages	1506:1555	To determine whether NanH is required for the Salmonella infection-dependent direction reversal, we constructed and characterized a nanH deletion mutant and found that NanH is partially required for Salmonella infection in primary murine macrophages.					
34662214	5	57	theme	glycocalyx	1037:1046	arg1	shift					1013:1017	the bacterial neuraminidase-associated compositional shift	960:1017	the bacterial neuraminidase-associated compositional shift	960:1017	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	5	58	theme	N-glycan	890:897	arg1	profiling					899:907	N-glycan profiling	890:907	N-glycan profiling	890:907	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	5	59	from	decrease	1066:1073	arg1	sialylated					1078:1087	sialylated	1078:1087	sialylated	1078:1087	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	5	59	from	decrease	1066:1073	arg1	structures					1137:1146	fucosylated and high mannose structures	1108:1146	fucosylated and high mannose structures	1108:1146	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	4	60	theme	macrophage	823:832	arg1	galvanotaxis					834:845	macrophage galvanotaxis	823:845	macrophage galvanotaxis	823:845	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	8	61	dep	type	1584:1587	arg1	Salmonella					1589:1598	wild type Salmonella	1579:1598	wild type Salmonella	1579:1598	However, compared to wild type Salmonella, infection with the nanH mutant only marginally reduced the cathode-oriented macrophage galvonotaxis, without canceling direction reversal.					
34662214	5	62	theme	fucosylated	1108:1118	arg1	structures					1137:1146	fucosylated and high mannose structures	1108:1146	fucosylated and high mannose structures	1108:1146	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	7	63	theme	primary	1530:1536	arg1	macrophages					1545:1555	primary murine macrophages	1530:1555	primary murine macrophages	1530:1555	To determine whether NanH is required for the Salmonella infection-dependent direction reversal, we constructed and characterized a nanH deletion mutant and found that NanH is partially required for Salmonella infection in primary murine macrophages.					
34662214	1	64	theme	gut	155:157	arg1	integrity					170:178	gut epithelium integrity	155:178	gut epithelium integrity	155:178	Salmonella invades and disrupts gut epithelium integrity, creating an infection-generated electric field that can drive directional migration of macrophages, a process called galvanotaxis.					
34662214	5	65	theme	compositional	999:1011	arg1	shift					1013:1017	the bacterial neuraminidase-associated compositional shift	960:1017	the bacterial neuraminidase-associated compositional shift	960:1017	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	6	66	theme	Salmonella	1153:1162	arg1	gene					1169:1172	The Salmonella nanH gene	1149:1172	The Salmonella nanH gene	1149:1172	The Salmonella nanH gene, encoding a putative neuraminidase, is required for invasion and internalization in a human colonic epithelial cell infection model.					
34662214	9	67	theme	neuraminidase-mediated	1793:1814	arg1	modification					1825:1836	neuraminidase-mediated N-glycan modification	1793:1836	neuraminidase-mediated N-glycan modification	1793:1836	Together, these findings strongly suggest that while neuraminidase-mediated N-glycan modification impaired both macrophage phagocytosis and galvanotaxis, yet to be defined mechanisms other than NanH may play a more important role in bioelectrical control of macrophage trafficking, which potentially triggers dissemination.					
34662214	4	68	with	cleavage	734:741	arg1	neuraminidase					792:804	a bacterial neuraminidase	780:804	a bacterial neuraminidase	780:804	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	1	69	theme	epithelium	159:168	arg1	integrity					170:178	gut epithelium integrity	155:178	gut epithelium integrity	155:178	Salmonella invades and disrupts gut epithelium integrity, creating an infection-generated electric field that can drive directional migration of macrophages, a process called galvanotaxis.					
34662214	1	70	theme	macrophages	268:278	arg1	process					283:289	a process	281:289	a process called galvanotaxis	281:309	Salmonella invades and disrupts gut epithelium integrity, creating an infection-generated electric field that can drive directional migration of macrophages, a process called galvanotaxis.					
34662214	1	70	theme	macrophages	268:278	arg1	migration					255:263	directional migration	243:263	directional migration of macrophages	243:278	Salmonella invades and disrupts gut epithelium integrity, creating an infection-generated electric field that can drive directional migration of macrophages, a process called galvanotaxis.					
34662214	7	71	theme	Salmonella	1353:1362	arg1	reversal					1394:1401	the Salmonella infection-dependent direction reversal	1349:1401	the Salmonella infection-dependent direction reversal	1349:1401	To determine whether NanH is required for the Salmonella infection-dependent direction reversal, we constructed and characterized a nanH deletion mutant and found that NanH is partially required for Salmonella infection in primary murine macrophages.					
34662214	7	72	theme	nanH	1439:1442	arg1	mutant					1453:1458	a nanH deletion mutant	1437:1458	a nanH deletion mutant	1437:1458	To determine whether NanH is required for the Salmonella infection-dependent direction reversal, we constructed and characterized a nanH deletion mutant and found that NanH is partially required for Salmonella infection in primary murine macrophages.					
34662214	5	73	theme	high	1124:1127	arg1	structures					1137:1146	fucosylated and high mannose structures	1108:1146	fucosylated and high mannose structures	1108:1146	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	7	74	theme	deletion	1444:1451	arg1	mutant					1453:1458	a nanH deletion mutant	1437:1458	a nanH deletion mutant	1437:1458	To determine whether NanH is required for the Salmonella infection-dependent direction reversal, we constructed and characterized a nanH deletion mutant and found that NanH is partially required for Salmonella infection in primary murine macrophages.					
34662214	3	75	theme	galvanotaxis	526:537	arg1	reversal					503:510	direction reversal	493:510	direction reversal of macrophage galvanotaxis	493:537	The force that drives direction reversal of macrophage galvanotaxis is not understood.					
34662214	0	76	theme	Macrophage	78:87	arg1	Galvanotaxis					89:100	Macrophage Galvanotaxis	78:100	Macrophage Galvanotaxis Independent of NanH	78:120	Surface Glycans Regulate Salmonella Infection-Dependent Directional Switch in Macrophage Galvanotaxis Independent of NanH.					
34662214	5	77	theme	mannose	1129:1135	arg1	structures					1137:1146	fucosylated and high mannose structures	1108:1146	fucosylated and high mannose structures	1108:1146	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	4	78	theme	acids	769:773	arg1	cleavage					734:741	cleavage	734:741	cleavage of surface-exposed sialic acids with a bacterial neuraminidase	734:804	One hypothesis is that Salmonella can alter the electrical properties of the macrophages by modifying host cell surface glycan composition, which is supported by the fact that cleavage of surface-exposed sialic acids with a bacterial neuraminidase severely impairs macrophage galvanotaxis, as well as phagocytosis.					
34662214	5	79	from	increase	1096:1103	arg1	sialylated					1078:1087	sialylated	1078:1087	sialylated	1078:1087	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
34662214	5	79	from	increase	1096:1103	arg1	structures					1137:1146	fucosylated and high mannose structures	1108:1146	fucosylated and high mannose structures	1108:1146	Here, we utilize N-glycan profiling by nanoLC-chip QTOF mass cytometry to characterize the bacterial neuraminidase-associated compositional shift of the macrophage glycocalyx, which revealed a decrease in sialylated and an increase in fucosylated and high mannose structures.					
32973204	3	0	gly	O-glycopeptide	689:702	arg2	O-glycopeptide					689:702	the glycodrosocin O-glycopeptide substrate	671:712	the glycodrosocin O-glycopeptide substrate	671:712	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	5	1	from	proteins	1146:1153	arg1	analysis					1089:1096	analysis	1089:1096	analysis of post-translational O-glycosylation events in proteins	1089:1153	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	5	1	from	proteins	1146:1153	arg1	mucins					1059:1064	mucins	1059:1064	mucins	1059:1064	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	3	2	with	complex	658:664	arg1	substrate					704:712	the glycodrosocin O-glycopeptide substrate	671:712	the glycodrosocin O-glycopeptide substrate	671:712	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	3	3	theme	catalytic	798:806	arg1	cycle					808:812	the catalytic cycle	794:812	the catalytic cycle	794:812	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	0	4	theme	O-glycopeptidase	64:79	arg1	OgpA					81:84	the human gut O-glycopeptidase OgpA	50:84	the human gut O-glycopeptidase OgpA from Akkermansia muciniphila	50:113	Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.					
32973204	5	5	theme	post-translational	1101:1118	arg1	events					1136:1141	post-translational O-glycosylation events	1101:1141	post-translational O-glycosylation events in proteins	1101:1153	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	4	6	theme	O-glycopeptide	835:848	arg1	chemistry					850:858	O-glycopeptide chemistry	835:858	O-glycopeptide chemistry	835:858	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	5	7	from	mucins	1059:1064	arg1	proteins					1146:1153	proteins	1146:1153	proteins	1146:1153	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	5	7	from	mucins	1059:1064	arg1	gut					1073:1075	the gut	1069:1075	the gut	1069:1075	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	2	8	theme	N-terminal	491:500	arg1	bond					486:489	the peptide bond	474:489	the peptide bond N-terminal to serine or threonine residues substituted with an O-glycan	474:561	In this work, we focus in OgpA from A. muciniphila, an O-glycopeptidase that exclusively hydrolyzes the peptide bond N-terminal to serine or threonine residues substituted with an O-glycan.					
32973204	5	9	theme	O-glycosylation	1120:1134	arg1	events					1136:1141	post-translational O-glycosylation events	1101:1141	post-translational O-glycosylation events in proteins	1101:1153	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	4	10	theme	enzyme	861:866	arg1	kinetics					868:875	enzyme kinetics	861:875	enzyme kinetics	861:875	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	4	11	theme	computational	882:894	arg1	methods					896:902	computational methods	882:902	computational methods	882:902	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	4	12	theme	specificity	966:976	arg1	mechanism					928:936	the molecular mechanism	914:936	the molecular mechanism of O-glycan recognition and specificity for OgpA	914:985	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	4	13	theme	molecular	918:926	arg1	mechanism					928:936	the molecular mechanism	914:936	the molecular mechanism of O-glycan recognition and specificity for OgpA	914:985	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	2	14	theme	peptide	478:484	arg1	bond					486:489	the peptide bond	474:489	the peptide bond N-terminal to serine or threonine residues substituted with an O-glycan	474:561	In this work, we focus in OgpA from A. muciniphila, an O-glycopeptidase that exclusively hydrolyzes the peptide bond N-terminal to serine or threonine residues substituted with an O-glycan.					
32973204	4	15	with	combination	818:828	arg1	chemistry					850:858	O-glycopeptide chemistry	835:858	O-glycopeptide chemistry	835:858	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	4	15	with	combination	818:828	arg1	kinetics					868:875	enzyme kinetics	861:875	enzyme kinetics	861:875	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	4	15	with	combination	818:828	arg1	methods					896:902	computational methods	882:902	computational methods	882:902	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	4	16	gly	O-glycopeptide	835:848	arg2	O-glycopeptide					835:848	O-glycopeptide chemistry	835:858	O-glycopeptide chemistry	835:858	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	1	17	located	found	180:184	arg2	bacterium					161:169	a mucin-degrading bacterium	143:169	a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system	143:371	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	1	17	located	found	180:184	arg1	gut					199:201	the human gut	189:201	the human gut	189:201	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	1	17	located	found	180:184	arg2	muciniphila					128:138	Akkermansia muciniphila	116:138	Akkermansia muciniphila	116:138	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	4	18	theme	O-glycan	941:948	arg1	recognition					950:960	O-glycan recognition	941:960	O-glycan recognition	941:960	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	3	19	theme	O-glycopeptide	689:702	arg1	substrate					704:712	the glycodrosocin O-glycopeptide substrate	671:712	the glycodrosocin O-glycopeptide substrate	671:712	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	3	20	with	product	722:728	arg1	substrate					704:712	the glycodrosocin O-glycopeptide substrate	671:712	the glycodrosocin O-glycopeptide substrate	671:712	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	1	21	theme	human	193:197	arg1	gut					199:201	the human gut	189:201	the human gut	189:201	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	0	22	theme	Structural	0:9	arg1	basis					11:15	Structural basis	0:15	Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.	0:114	Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.					
32973204	3	23	with	structures	611:620	arg1	substrate					704:712	the glycodrosocin O-glycopeptide substrate	671:712	the glycodrosocin O-glycopeptide substrate	671:712	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	3	24	theme	unliganded	629:638	arg1	form					640:643	the unliganded form	625:643	the unliganded form of OgpA	625:651	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	0	25	theme	mammalian	20:28	arg1	processing					36:45	mammalian mucin processing	20:45	mammalian mucin processing	20:45	Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.					
32973204	1	26	theme	mucus	282:286	arg1	thickness					288:296	mucus thickness	282:296	mucus thickness	282:296	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	1	27	theme	immune	359:364	arg1	system					366:371	the immune system	355:371	the immune system	355:371	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	0	28	theme	processing	36:45	arg1	basis					11:15	Structural basis	0:15	Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.	0:114	Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.					
32973204	1	29	theme	thickness	288:296	arg1	regulation					268:277	the regulation	264:277	the regulation of mucus thickness and gut barrier integrity	264:322	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	1	30	theme	system	366:371	arg1	modulation					341:350	the modulation	337:350	the modulation of the immune system	337:371	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	0	31	theme	mucin	30:34	arg1	processing					36:45	mammalian mucin processing	20:45	mammalian mucin processing	20:45	Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.					
32973204	5	32	theme	events	1136:1141	arg1	analysis					1089:1096	analysis	1089:1096	analysis of post-translational O-glycosylation events in proteins	1089:1153	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	5	32	theme	events	1136:1141	arg1	mucins					1059:1064	mucins	1059:1064	mucins	1059:1064	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	1	33	theme	beneficial	219:228	arg1	effect					230:235	a beneficial effect	217:235	a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system	217:371	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	5	34	from	analysis	1089:1096	arg1	proteins					1146:1153	proteins	1146:1153	proteins	1146:1153	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	5	34	from	analysis	1089:1096	arg1	gut					1073:1075	the gut	1069:1075	the gut	1069:1075	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	1	35	theme	gut	302:304	arg1	integrity					314:322	gut barrier integrity	302:322	gut barrier integrity	302:322	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	4	36	theme	recognition	950:960	arg1	mechanism					928:936	the molecular mechanism	914:936	the molecular mechanism of O-glycan recognition and specificity for OgpA	914:985	In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA.					
32973204	3	37	theme	glycodrosocin	675:687	arg1	substrate					704:712	the glycodrosocin O-glycopeptide substrate	671:712	the glycodrosocin O-glycopeptide substrate	671:712	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	1	38	theme	Akkermansia	116:126	arg1	bacterium					161:169	a mucin-degrading bacterium	143:169	a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system	143:371	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	1	38	theme	Akkermansia	116:126	arg1	muciniphila					128:138	Akkermansia muciniphila	116:138	Akkermansia muciniphila	116:138	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	1	39	theme	barrier	306:312	arg1	integrity					314:322	gut barrier integrity	302:322	gut barrier integrity	302:322	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	3	40	theme	OgpA	648:651	arg1	form					640:643	the unliganded form	625:643	the unliganded form of OgpA	625:651	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	5	41	from	events	1136:1141	arg1	proteins					1146:1153	proteins	1146:1153	proteins	1146:1153	The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins.					
32973204	1	42	theme	integrity	314:322	arg1	regulation					268:277	the regulation	264:277	the regulation of mucus thickness and gut barrier integrity	264:322	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	1	43	from	effect	230:235	arg1	health					240:245	health	240:245	health	240:245	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	1	43	from	effect	230:235	arg1	modulation					341:350	the modulation	337:350	the modulation of the immune system	337:371	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	0	44	from	muciniphila	103:113	arg1	OgpA					81:84	the human gut O-glycopeptidase OgpA	50:84	the human gut O-glycopeptidase OgpA from Akkermansia muciniphila	50:113	Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.					
32973204	3	45	theme	X-ray	597:601	arg1	structures					611:620	the high-resolution X-ray crystal structures	577:620	the high-resolution X-ray crystal structures of the unliganded form of OgpA	577:651	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	2	46	dep	serine	505:510	arg1	residues					525:532	residues	525:532	residues	525:532	In this work, we focus in OgpA from A. muciniphila, an O-glycopeptidase that exclusively hydrolyzes the peptide bond N-terminal to serine or threonine residues substituted with an O-glycan.					
32973204	3	47	theme	comprehensive	743:755	arg1	snapshots					764:772	snapshots	764:772	snapshots of the enzyme	764:786	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	3	47	theme	comprehensive	743:755	arg1	set					757:759	a comprehensive set	741:759	a comprehensive set of snapshots of the enzyme along the catalytic cycle	741:812	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	3	48	theme	form	640:643	arg1	structures					611:620	the high-resolution X-ray crystal structures	577:620	the high-resolution X-ray crystal structures of the unliganded form of OgpA	577:651	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	3	48	theme	form	640:643	arg1	product					722:728	its product	718:728	its product	718:728	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	3	48	theme	form	640:643	arg1	complex					658:664	the complex	654:664	the complex with the glycodrosocin O-glycopeptide substrate	654:712	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	3	49	theme	crystal	603:609	arg1	structures					611:620	the high-resolution X-ray crystal structures	577:620	the high-resolution X-ray crystal structures of the unliganded form of OgpA	577:651	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	3	50	theme	enzyme	781:786	arg1	snapshots					764:772	snapshots	764:772	snapshots of the enzyme	764:786	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	0	51	theme	gut	60:62	arg1	OgpA					81:84	the human gut O-glycopeptidase OgpA	50:84	the human gut O-glycopeptidase OgpA from Akkermansia muciniphila	50:113	Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.					
32973204	1	52	theme	mucin-degrading	145:159	arg1	bacterium					161:169	a mucin-degrading bacterium	143:169	a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system	143:371	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	1	52	theme	mucin-degrading	145:159	arg1	muciniphila					128:138	Akkermansia muciniphila	116:138	Akkermansia muciniphila	116:138	Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system.					
32973204	3	53	theme	snapshots	764:772	arg1	snapshots					764:772	snapshots	764:772	snapshots of the enzyme	764:786	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	3	53	theme	snapshots	764:772	arg1	set					757:759	a comprehensive set	741:759	a comprehensive set of snapshots of the enzyme along the catalytic cycle	741:812	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32973204	0	54	theme	human	54:58	arg1	OgpA					81:84	the human gut O-glycopeptidase OgpA	50:84	the human gut O-glycopeptidase OgpA from Akkermansia muciniphila	50:113	Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.					
32973204	3	55	theme	high-resolution	581:595	arg1	structures					611:620	the high-resolution X-ray crystal structures	577:620	the high-resolution X-ray crystal structures of the unliganded form of OgpA	577:651	We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle.					
32590086	8	0	dep	in	1204:1205	arg1	vitro					1207:1211	vitro	1207:1211	vitro	1207:1211	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	1	1	theme	hot	160:162	arg1	extraction					170:179	a hot water extraction	158:179	a hot water extraction	158:179	Following ultrasonication combined with a hot water extraction, a new type of bioactive polysaccharide, SPS-3-1, was purified from Spirulina using ultrafiltration centrifugation and gel filtration chromatography.					
32590086	2	2	theme	magnetic	514:521	arg1	resonance					523:531	nuclear magnetic resonance	506:531	nuclear magnetic resonance	506:531	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	8	3	theme	cells	1240:1244	arg1	growth					1213:1218	the in vitro growth	1200:1218	the in vitro growth of Caco-2 and HepG2 cells	1200:1244	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	1	4	theme	water	164:168	arg1	extraction					170:179	a hot water extraction	158:179	a hot water extraction	158:179	Following ultrasonication combined with a hot water extraction, a new type of bioactive polysaccharide, SPS-3-1, was purified from Spirulina using ultrafiltration centrifugation and gel filtration chromatography.					
32590086	2	5	theme	atomic	538:543	arg1	microscopy					551:560	atomic force microscopy	538:560	atomic force microscopy	538:560	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	7	6	theme	transepithelial	995:1009	arg1	resistance					1022:1031	transepithelial electrical resistance	995:1031	transepithelial electrical resistance	995:1031	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	7	6	theme	transepithelial	995:1009	arg1	marker					1061:1066	a tight junction integrity marker	1034:1066	a tight junction integrity marker	1034:1066	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	4	7	theme	components	773:782	arg1	1:0.70:1.03:2.1:6.59					787:806	1:0.70:1.03:2.1:6.59	787:806	1:0.70:1.03:2.1:6.59	787:806	The molar ratio of these components is 1:0.70:1.03:2.1:6.59.					
32590086	4	7	theme	components	773:782	arg1	ratio					758:762	The molar ratio	748:762	The molar ratio of these components	748:782	The molar ratio of these components is 1:0.70:1.03:2.1:6.59.					
32590086	8	8	from	effect	1130:1135	arg1	proliferation					1156:1168	cell proliferation	1151:1168	cell proliferation	1151:1168	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	2	9	theme	force	545:549	arg1	microscopy					551:560	atomic force microscopy	538:560	atomic force microscopy	538:560	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	6	10	theme	34.132 nm	912:920	arg1	height					928:933	a height	926:933	a height of 819.169 pm	926:947	SPS-3-1 has a linear filament structure with a width of 34.132 nm and a height of 819.169 pm.					
32590086	6	10	theme	34.132 nm	912:920	arg1	width					903:907	a width	901:907	a width of 34.132 nm	901:920	SPS-3-1 has a linear filament structure with a width of 34.132 nm and a height of 819.169 pm.					
32590086	8	11	theme	in	1204:1205	arg1	growth					1213:1218	the in vitro growth	1200:1218	the in vitro growth of Caco-2 and HepG2 cells	1200:1244	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	8	12	theme	566.67 μg/mL	1262:1273	arg1	IC50					1254:1257	an IC50	1251:1257	an IC50 of 566.67 μg/mL and 1078.95 μg/mL	1251:1291	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	6	13	with	structure	886:894	arg1	height					928:933	a height	926:933	a height of 819.169 pm	926:947	SPS-3-1 has a linear filament structure with a width of 34.132 nm and a height of 819.169 pm.					
32590086	6	13	with	structure	886:894	arg1	width					903:907	a width	901:907	a width of 34.132 nm	901:920	SPS-3-1 has a linear filament structure with a width of 34.132 nm and a height of 819.169 pm.					
32590086	0	14	theme	Caco-2	104:109	arg1	cells					111:115	Caco-2 cells	104:115	Caco-2 cells	104:115	Effects of the polysaccharide SPS-3-1 purified from Spirulina on barrier integrity and proliferation of Caco-2 cells.					
32590086	8	15	theme	HepG2	1234:1238	arg1	cells					1240:1244	Caco-2 and HepG2 cells	1223:1244	cells	1240:1244	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	0	16	theme	cells	111:115	arg1	integrity					73:81	barrier integrity	65:81	barrier integrity	65:81	Effects of the polysaccharide SPS-3-1 purified from Spirulina on barrier integrity and proliferation of Caco-2 cells.					
32590086	0	16	theme	cells	111:115	arg1	proliferation					87:99	proliferation	87:99	proliferation	87:99	Effects of the polysaccharide SPS-3-1 purified from Spirulina on barrier integrity and proliferation of Caco-2 cells.					
32590086	5	17	theme	SPS-3-1	833:839	arg1	weight					823:828	The molecular weight	809:828	The molecular weight of SPS-3-1	809:839	The molecular weight of SPS-3-1 is 623.02 kDa.					
32590086	5	17	theme	SPS-3-1	833:839	arg1	623.02 kDa					844:853	623.02 kDa	844:853	623.02 kDa	844:853	The molecular weight of SPS-3-1 is 623.02 kDa.					
32590086	2	18	dep	transform	473:481	arg1	infrared					483:490	infrared	483:490	transform infrared spectroscopy	473:503	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	8	19	theme	effect	1130:1135	arg1	Analysis					1114:1121	Analysis	1114:1121	Analysis of the effect of SPS-3-1 on cell proliferation	1114:1168	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	2	20	theme	permeation	397:406	arg1	chromatography					408:421	high performance gel permeation chromatography	376:421	high performance gel permeation chromatography	376:421	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	1	21	theme	ultrafiltration	265:279	arg1	centrifugation					281:294	ultrafiltration centrifugation	265:294	ultrafiltration centrifugation	265:294	Following ultrasonication combined with a hot water extraction, a new type of bioactive polysaccharide, SPS-3-1, was purified from Spirulina using ultrafiltration centrifugation and gel filtration chromatography.					
32590086	2	22	dep	fourier	465:471	arg1	transform					473:481	transform	473:481	transform infrared spectroscopy	473:503	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	4	23	theme	molar	752:756	arg1	1:0.70:1.03:2.1:6.59					787:806	1:0.70:1.03:2.1:6.59	787:806	1:0.70:1.03:2.1:6.59	787:806	The molar ratio of these components is 1:0.70:1.03:2.1:6.59.					
32590086	4	23	theme	molar	752:756	arg1	ratio					758:762	The molar ratio	748:762	The molar ratio of these components	748:782	The molar ratio of these components is 1:0.70:1.03:2.1:6.59.					
32590086	2	24	theme	gel	393:395	arg1	chromatography					408:421	high performance gel permeation chromatography	376:421	high performance gel permeation chromatography	376:421	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	0	25	theme	polysaccharide	15:28	arg1	SPS-3-1					30:36	the polysaccharide SPS-3-1	11:36	the polysaccharide SPS-3-1	11:36	Effects of the polysaccharide SPS-3-1 purified from Spirulina on barrier integrity and proliferation of Caco-2 cells.					
32590086	7	26	theme	monolayer	1097:1105	arg1	model					1107:1111	a Caco-2 intestinal cell monolayer model	1072:1111	a Caco-2 intestinal cell monolayer model	1072:1111	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	8	27	theme	Caco-2	1223:1228	arg1	cells					1240:1244	Caco-2 and HepG2 cells	1223:1244	cells	1240:1244	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	3	28	theme	homogeneous	588:598	arg1	polysaccharide					608:621	a homogeneous β-pyran polysaccharide	586:621	a homogeneous β-pyran polysaccharide	586:621	SPS-3-1 is a homogeneous β-pyran polysaccharide with 1 → 2, 1 → 3, and 1 → 4 glycosyl bonds, mainly composed of d-ribose, l-rhamnose, l-arabinose, l-foucose, and d-glucose.					
32590086	3	28	theme	homogeneous	588:598	arg1	SPS-3-1					575:581	SPS-3-1	575:581	SPS-3-1	575:581	SPS-3-1 is a homogeneous β-pyran polysaccharide with 1 → 2, 1 → 3, and 1 → 4 glycosyl bonds, mainly composed of d-ribose, l-rhamnose, l-arabinose, l-foucose, and d-glucose.					
32590086	2	29	theme	periodate	444:452	arg1	oxidation					454:462	periodate oxidation	444:462	periodate oxidation	444:462	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	3	30	theme	β-pyran	600:606	arg1	polysaccharide					608:621	a homogeneous β-pyran polysaccharide	586:621	a homogeneous β-pyran polysaccharide	586:621	SPS-3-1 is a homogeneous β-pyran polysaccharide with 1 → 2, 1 → 3, and 1 → 4 glycosyl bonds, mainly composed of d-ribose, l-rhamnose, l-arabinose, l-foucose, and d-glucose.					
32590086	3	30	theme	β-pyran	600:606	arg1	SPS-3-1					575:581	SPS-3-1	575:581	SPS-3-1	575:581	SPS-3-1 is a homogeneous β-pyran polysaccharide with 1 → 2, 1 → 3, and 1 → 4 glycosyl bonds, mainly composed of d-ribose, l-rhamnose, l-arabinose, l-foucose, and d-glucose.					
32590086	2	31	theme	nuclear	506:512	arg1	resonance					523:531	nuclear magnetic resonance	506:531	nuclear magnetic resonance	506:531	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	1	32	theme	gel	300:302	arg1	chromatography					315:328	gel filtration chromatography	300:328	gel filtration chromatography	300:328	Following ultrasonication combined with a hot water extraction, a new type of bioactive polysaccharide, SPS-3-1, was purified from Spirulina using ultrafiltration centrifugation and gel filtration chromatography.					
32590086	7	33	theme	intestinal	1081:1090	arg1	model					1107:1111	a Caco-2 intestinal cell monolayer model	1072:1111	a Caco-2 intestinal cell monolayer model	1072:1111	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	1	34	theme	new	184:186	arg1	SPS-3-1					222:228	SPS-3-1	222:228	SPS-3-1	222:228	Following ultrasonication combined with a hot water extraction, a new type of bioactive polysaccharide, SPS-3-1, was purified from Spirulina using ultrafiltration centrifugation and gel filtration chromatography.					
32590086	1	34	theme	new	184:186	arg1	type					188:191	a new type	182:191	a new type of bioactive polysaccharide	182:219	Following ultrasonication combined with a hot water extraction, a new type of bioactive polysaccharide, SPS-3-1, was purified from Spirulina using ultrafiltration centrifugation and gel filtration chromatography.					
32590086	1	35	theme	bioactive	196:204	arg1	polysaccharide					206:219	bioactive polysaccharide	196:219	bioactive polysaccharide	196:219	Following ultrasonication combined with a hot water extraction, a new type of bioactive polysaccharide, SPS-3-1, was purified from Spirulina using ultrafiltration centrifugation and gel filtration chromatography.					
32590086	0	36	theme	SPS-3-1	30:36	arg1	Effects					0:6	Effects	0:6	Effects of the polysaccharide SPS-3-1	0:36	Effects of the polysaccharide SPS-3-1 purified from Spirulina on barrier integrity and proliferation of Caco-2 cells.					
32590086	1	37	theme	filtration	304:313	arg1	chromatography					315:328	gel filtration chromatography	300:328	gel filtration chromatography	300:328	Following ultrasonication combined with a hot water extraction, a new type of bioactive polysaccharide, SPS-3-1, was purified from Spirulina using ultrafiltration centrifugation and gel filtration chromatography.					
32590086	5	38	theme	molecular	813:821	arg1	weight					823:828	The molecular weight	809:828	The molecular weight of SPS-3-1	809:839	The molecular weight of SPS-3-1 is 623.02 kDa.					
32590086	5	38	theme	molecular	813:821	arg1	623.02 kDa					844:853	623.02 kDa	844:853	623.02 kDa	844:853	The molecular weight of SPS-3-1 is 623.02 kDa.					
32590086	8	39	from	proliferation	1156:1168	arg1	Analysis					1114:1121	Analysis	1114:1121	Analysis of the effect of SPS-3-1 on cell proliferation	1114:1168	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	3	40	with	polysaccharide	608:621	arg1	1 → 3					635:639	1 → 3	635:639	1 → 3	635:639	SPS-3-1 is a homogeneous β-pyran polysaccharide with 1 → 2, 1 → 3, and 1 → 4 glycosyl bonds, mainly composed of d-ribose, l-rhamnose, l-arabinose, l-foucose, and d-glucose.					
32590086	3	40	with	polysaccharide	608:621	arg1	1 → 2					628:632	1 → 2	628:632	1 → 2	628:632	SPS-3-1 is a homogeneous β-pyran polysaccharide with 1 → 2, 1 → 3, and 1 → 4 glycosyl bonds, mainly composed of d-ribose, l-rhamnose, l-arabinose, l-foucose, and d-glucose.					
32590086	3	40	with	polysaccharide	608:621	arg1	bonds					661:665	1 → 4 glycosyl bonds	646:665	1 → 4 glycosyl bonds	646:665	SPS-3-1 is a homogeneous β-pyran polysaccharide with 1 → 2, 1 → 3, and 1 → 4 glycosyl bonds, mainly composed of d-ribose, l-rhamnose, l-arabinose, l-foucose, and d-glucose.					
32590086	7	41	theme	integrity	1051:1059	arg1	resistance					1022:1031	transepithelial electrical resistance	995:1031	transepithelial electrical resistance	995:1031	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	7	41	theme	integrity	1051:1059	arg1	marker					1061:1066	a tight junction integrity marker	1034:1066	a tight junction integrity marker	1034:1066	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	1	42	theme	polysaccharide	206:219	arg1	SPS-3-1					222:228	SPS-3-1	222:228	SPS-3-1	222:228	Following ultrasonication combined with a hot water extraction, a new type of bioactive polysaccharide, SPS-3-1, was purified from Spirulina using ultrafiltration centrifugation and gel filtration chromatography.					
32590086	1	42	theme	polysaccharide	206:219	arg1	type					188:191	a new type	182:191	a new type of bioactive polysaccharide	182:219	Following ultrasonication combined with a hot water extraction, a new type of bioactive polysaccharide, SPS-3-1, was purified from Spirulina using ultrafiltration centrifugation and gel filtration chromatography.					
32590086	6	43	theme	filament	877:884	arg1	structure					886:894	a linear filament structure	868:894	a linear filament structure with a width of 34.132 nm and a height of 819.169 pm	868:947	SPS-3-1 has a linear filament structure with a width of 34.132 nm and a height of 819.169 pm.					
32590086	7	44	theme	cell	1092:1095	arg1	model					1107:1111	a Caco-2 intestinal cell monolayer model	1072:1111	a Caco-2 intestinal cell monolayer model	1072:1111	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	7	45	theme	Caco-2	1074:1079	arg1	model					1107:1111	a Caco-2 intestinal cell monolayer model	1072:1111	a Caco-2 intestinal cell monolayer model	1072:1111	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	6	46	theme	linear	870:875	arg1	structure					886:894	a linear filament structure	868:894	a linear filament structure with a width of 34.132 nm and a height of 819.169 pm	868:947	SPS-3-1 has a linear filament structure with a width of 34.132 nm and a height of 819.169 pm.					
32590086	7	47	theme	tight	1036:1040	arg1	resistance					1022:1031	transepithelial electrical resistance	995:1031	transepithelial electrical resistance	995:1031	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	7	47	theme	tight	1036:1040	arg1	marker					1061:1066	a tight junction integrity marker	1034:1066	a tight junction integrity marker	1034:1066	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	2	48	theme	SPS-3-1	348:354	arg1	structure					335:343	The structure	331:343	The structure of SPS-3-1	331:354	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	3	49	theme	1 → 4	646:650	arg1	bonds					661:665	1 → 4 glycosyl bonds	646:665	1 → 4 glycosyl bonds	646:665	SPS-3-1 is a homogeneous β-pyran polysaccharide with 1 → 2, 1 → 3, and 1 → 4 glycosyl bonds, mainly composed of d-ribose, l-rhamnose, l-arabinose, l-foucose, and d-glucose.					
32590086	3	50	theme	glycosyl	652:659	arg1	bonds					661:665	1 → 4 glycosyl bonds	646:665	1 → 4 glycosyl bonds	646:665	SPS-3-1 is a homogeneous β-pyran polysaccharide with 1 → 2, 1 → 3, and 1 → 4 glycosyl bonds, mainly composed of d-ribose, l-rhamnose, l-arabinose, l-foucose, and d-glucose.					
32590086	7	51	theme	junction	1042:1049	arg1	resistance					1022:1031	transepithelial electrical resistance	995:1031	transepithelial electrical resistance	995:1031	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	7	51	theme	junction	1042:1049	arg1	marker					1061:1066	a tight junction integrity marker	1034:1066	a tight junction integrity marker	1034:1066	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	8	52	theme	SPS-3-1	1140:1146	arg1	effect					1130:1135	the effect	1126:1135	the effect of SPS-3-1 on cell proliferation	1126:1168	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	6	53	contain	has	864:866	arg1	SPS-3-1					856:862	SPS-3-1	856:862	SPS-3-1	856:862	SPS-3-1 has a linear filament structure with a width of 34.132 nm and a height of 819.169 pm.					
32590086	6	53	contain	has	864:866	arg2	structure					886:894	a linear filament structure	868:894	a linear filament structure with a width of 34.132 nm and a height of 819.169 pm	868:947	SPS-3-1 has a linear filament structure with a width of 34.132 nm and a height of 819.169 pm.					
32590086	0	54	theme	barrier	65:71	arg1	integrity					73:81	barrier integrity	65:81	barrier integrity	65:81	Effects of the polysaccharide SPS-3-1 purified from Spirulina on barrier integrity and proliferation of Caco-2 cells.					
32590086	2	55	theme	gas	424:426	arg1	chromatography					428:441	gas chromatography	424:441	gas chromatography	424:441	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	8	56	theme	1078.95 μg/mL	1279:1291	arg1	IC50					1254:1257	an IC50	1251:1257	an IC50 of 566.67 μg/mL and 1078.95 μg/mL	1251:1291	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	2	57	theme	performance	381:391	arg1	chromatography					408:421	high performance gel permeation chromatography	376:421	high performance gel permeation chromatography	376:421	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	8	58	from	Analysis	1114:1121	arg1	proliferation					1156:1168	cell proliferation	1151:1168	cell proliferation	1151:1168	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	8	59	theme	cell	1151:1154	arg1	proliferation					1156:1168	cell proliferation	1151:1168	cell proliferation	1151:1168	Analysis of the effect of SPS-3-1 on cell proliferation showed that SPS-3-1 inhibited the in vitro growth of Caco-2 and HepG2 cells with an IC50 of 566.67 μg/mL and 1078.95 μg/mL, respectively.					
32590086	6	60	theme	819.169 pm	938:947	arg1	height					928:933	a height	926:933	a height of 819.169 pm	926:947	SPS-3-1 has a linear filament structure with a width of 34.132 nm and a height of 819.169 pm.					
32590086	6	60	theme	819.169 pm	938:947	arg1	width					903:907	a width	901:907	a width of 34.132 nm	901:920	SPS-3-1 has a linear filament structure with a width of 34.132 nm and a height of 819.169 pm.					
32590086	2	61	dep	chromatography	408:421	arg1	performance					562:572	performance	562:572	performance	562:572	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
32590086	7	62	theme	electrical	1011:1020	arg1	resistance					1022:1031	transepithelial electrical resistance	995:1031	transepithelial electrical resistance	995:1031	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	7	62	theme	electrical	1011:1020	arg1	marker					1061:1066	a tight junction integrity marker	1034:1066	a tight junction integrity marker	1034:1066	We found that SPS-3-1 significantly enhanced transepithelial electrical resistance, a tight junction integrity marker, in a Caco-2 intestinal cell monolayer model.					
32590086	2	63	theme	high	376:379	arg1	chromatography					408:421	high performance gel permeation chromatography	376:421	high performance gel permeation chromatography	376:421	The structure of SPS-3-1 was determined with high performance gel permeation chromatography, gas chromatography, periodate oxidation, fourier transform infrared spectroscopy, nuclear magnetic resonance, and atomic force microscopy performance.					
33396746	7	0	theme	T156R	1101:1105	arg1	mutants					1117:1123	Single Q101H, T156R and E196K mutants	1087:1123	Single Q101H, T156R and E196K mutants	1087:1123	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	6	1	theme	glycolipids	1074:1084	arg1	lectins					1007:1013	lectins	1007:1013	lectins	1007:1013	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	6	1	theme	glycolipids	1074:1084	arg1	assignment					1027:1036	structural assignment	1016:1036	structural assignment of N- and O-glycans	1016:1056	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	6	1	theme	glycolipids	1074:1084	arg1	analysis					1062:1069	analysis	1062:1069	analysis of glycolipids	1062:1084	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	4	2	from	effects	663:669	arg1	functionality					753:765	the CST's functionality	743:765	the CST's functionality	743:765	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	1	3	theme	glycosylation	241:253	arg1	pathways					255:262	glycosylation pathways	241:262	glycosylation pathways	241:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	5	4	theme	mutations	880:888	arg1	none					856:859	none	856:859	none of the CDG-causing mutations	856:888	As shown by microscopic studies, none of the CDG-causing mutations affected the protein's proper localization in the Golgi apparatus.					
33396746	8	5	from	differences	1343:1353	arg1	ability					1362:1368	the ability	1358:1368	the ability of CST variants to form dimers	1358:1399	We also revealed differences in the ability of CST variants to form dimers.					
33396746	0	6	theme	Acid	103:106	arg1	Transporter					108:118	the CMP-Sialic Acid Transporter	88:118	the CMP-Sialic Acid Transporter	88:118	Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter.					
33396746	9	7	theme	SLC35A1-CDG	1485:1495	arg1	cases					1497:1501	SLC35A1-CDG cases	1485:1501	SLC35A1-CDG cases	1485:1501	The results of this study improve our understanding of the molecular background of SLC35A1-CDG cases.					
33396746	8	8	theme	variants	1377:1384	arg1	ability					1362:1368	the ability	1358:1368	the ability of CST variants to form dimers	1358:1399	We also revealed differences in the ability of CST variants to form dimers.					
33396746	3	9	theme	described	523:531	arg1	cases					545:549	two previously described SLC35A1-CDG cases	508:549	two previously described SLC35A1-CDG cases	508:549	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	6	10	theme	Cellular	957:964	arg1	glycophenotypes					966:980	Cellular glycophenotypes	957:980	Cellular glycophenotypes	957:980	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	0	11	theme	CMP-Sialic	92:101	arg1	Transporter					108:118	the CMP-Sialic Acid Transporter	88:118	the CMP-Sialic Acid Transporter	88:118	Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter.					
33396746	3	12	theme	SLC35A1-CDG	533:543	arg1	cases					545:549	two previously described SLC35A1-CDG cases	508:549	two previously described SLC35A1-CDG cases	508:549	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	3	13	from	properties	494:503	arg1	cases					545:549	two previously described SLC35A1-CDG cases	508:549	two previously described SLC35A1-CDG cases	508:549	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	2	14	theme	CDG-causing	287:297	arg1	mutations					299:307	CDG-causing mutations	287:307	CDG-causing mutations in the SLC35A1 gene encoding the CMP-sialic acid transporter (CST)	287:374	Five patients bearing CDG-causing mutations in the SLC35A1 gene encoding the CMP-sialic acid transporter (CST) have been reported to date.					
33396746	9	15	theme	study	1422:1426	arg1	results					1406:1412	The results	1402:1412	The results of this study	1402:1426	The results of this study improve our understanding of the molecular background of SLC35A1-CDG cases.					
33396746	7	16	theme	E196K	1240:1244	arg1	mutation					1246:1253	a single T156R or E196K mutation	1222:1253	mutation	1246:1253	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	2	17	theme	acid	353:356	arg1	CST					371:373	CST	371:373	CST	371:373	Five patients bearing CDG-causing mutations in the SLC35A1 gene encoding the CMP-sialic acid transporter (CST) have been reported to date.					
33396746	2	17	theme	acid	353:356	arg1	transporter					358:368	the CMP-sialic acid transporter	338:368	the CMP-sialic acid transporter (CST)	338:374	Five patients bearing CDG-causing mutations in the SLC35A1 gene encoding the CMP-sialic acid transporter (CST) have been reported to date.					
33396746	5	18	theme	Golgi	940:944	arg1	apparatus					946:954	the Golgi apparatus	936:954	the Golgi apparatus	936:954	As shown by microscopic studies, none of the CDG-causing mutations affected the protein's proper localization in the Golgi apparatus.					
33396746	6	19	theme	O-glycans	1048:1056	arg1	lectins					1007:1013	lectins	1007:1013	lectins	1007:1013	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	6	19	theme	O-glycans	1048:1056	arg1	assignment					1027:1036	structural assignment	1016:1036	structural assignment of N- and O-glycans	1016:1056	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	6	19	theme	O-glycans	1048:1056	arg1	analysis					1062:1069	analysis	1062:1069	analysis of glycolipids	1062:1084	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	4	20	dep	T156R	714:718	arg1	mutations					730:738	mutations	730:738	mutations	730:738	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	4	21	theme	HEK293T	808:814	arg1	cells					816:820	CST-deficient HEK293T cells	794:820	CST-deficient HEK293T cells	794:820	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	3	22	theme	heterozygous	622:633	arg1	T156R/E196K					645:655	T156R/E196K	645:655	T156R/E196K	645:655	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	3	22	theme	heterozygous	622:633	arg1	mutation					635:642	a compound heterozygous mutation	611:642	a compound heterozygous mutation (T156R/E196K)	611:656	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	9	23	theme	cases	1497:1501	arg1	background					1471:1480	the molecular background	1457:1480	the molecular background of SLC35A1-CDG cases	1457:1501	The results of this study improve our understanding of the molecular background of SLC35A1-CDG cases.					
33396746	5	24	theme	microscopic	835:845	arg1	studies					847:853	microscopic studies	835:853	microscopic studies	835:853	As shown by microscopic studies, none of the CDG-causing mutations affected the protein's proper localization in the Golgi apparatus.					
33396746	0	25	theme	Novel	0:4	arg1	Insights					6:13	Novel Insights	0:13	Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter	0:118	Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter.					
33396746	7	26	from	effect	1212:1217	arg1	functionality					1266:1278	the CST functionality	1258:1278	the CST functionality	1258:1278	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	2	27	theme	SLC35A1	316:322	arg1	gene					324:327	the SLC35A1 gene	312:327	the SLC35A1 gene encoding the CMP-sialic acid transporter (CST)	312:374	Five patients bearing CDG-causing mutations in the SLC35A1 gene encoding the CMP-sialic acid transporter (CST) have been reported to date.					
33396746	1	28	theme	rare	180:183	arg1	diseases					207:214	rare genetic and metabolic diseases	180:214	rare genetic and metabolic diseases caused by alterations in glycosylation pathways	180:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	0	29	theme	Selected	20:27	arg1	Mutations					45:53	Selected Disease-Causing Mutations	20:53	Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter	20:118	Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter.					
33396746	4	30	from	combination	699:709	arg1	functionality					753:765	the CST's functionality	743:765	the CST's functionality	743:765	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	3	31	dep	properties	494:503	arg1	one					552:554	one	552:554	one	552:554	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	3	31	dep	properties	494:503	arg1	another					593:599	another	593:599	another	593:599	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	1	32	theme	genetic	185:191	arg1	diseases					207:214	rare genetic and metabolic diseases	180:214	rare genetic and metabolic diseases caused by alterations in glycosylation pathways	180:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	3	33	theme	compound	613:620	arg1	T156R/E196K					645:655	T156R/E196K	645:655	T156R/E196K	645:655	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	3	33	theme	compound	613:620	arg1	mutation					635:642	a compound heterozygous mutation	611:642	a compound heterozygous mutation (T156R/E196K)	611:656	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	4	34	theme	T156R	714:718	arg1	effects					663:669	The effects	659:669	The effects of single mutations	659:689	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	4	34	theme	T156R	714:718	arg1	combination					699:709	the combination	695:709	the combination of T156R and E196K mutations	695:738	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	7	35	theme	CST	1262:1264	arg1	functionality					1266:1278	the CST functionality	1258:1278	the CST functionality	1258:1278	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	4	36	theme	single	674:679	arg1	mutations					681:689	single mutations	674:689	single mutations	674:689	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	3	37	theme	specific	434:441	arg1	mutations					443:451	specific mutations	434:451	specific mutations in the SLC35A1 gene	434:471	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	1	38	theme	metabolic	197:205	arg1	diseases					207:214	rare genetic and metabolic diseases	180:214	rare genetic and metabolic diseases caused by alterations in glycosylation pathways	180:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	0	39	theme	Disease-Causing	29:43	arg1	Mutations					45:53	Selected Disease-Causing Mutations	20:53	Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter	20:118	Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter.					
33396746	4	40	theme	E196K	724:728	arg1	effects					663:669	The effects	659:669	The effects of single mutations	659:689	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	4	40	theme	E196K	724:728	arg1	combination					699:709	the combination	695:709	the combination of T156R and E196K mutations	695:738	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	1	41	theme	diseases	207:214	arg1	disorders					132:140	Congenital disorders	121:140	Congenital disorders of glycosylation (CDG)	121:163	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	1	41	theme	diseases	207:214	arg1	group					171:175	a group	169:175	a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways	169:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	1	41	theme	diseases	207:214	arg1	diseases					207:214	rare genetic and metabolic diseases	180:214	rare genetic and metabolic diseases caused by alterations in glycosylation pathways	180:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	7	42	theme	E196K	1111:1115	arg1	mutants					1117:1123	Single Q101H, T156R and E196K mutants	1087:1123	Single Q101H, T156R and E196K mutants	1087:1123	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	1	43	from	alterations	226:236	arg1	pathways					255:262	glycosylation pathways	241:262	glycosylation pathways	241:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	4	44	theme	CST-deficient	794:806	arg1	cells					816:820	CST-deficient HEK293T cells	794:820	CST-deficient HEK293T cells	794:820	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	3	45	from	mutations	443:451	arg1	gene					468:471	the SLC35A1 gene	456:471	the SLC35A1 gene	456:471	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	1	46	theme	Congenital	121:130	arg1	diseases					207:214	rare genetic and metabolic diseases	180:214	rare genetic and metabolic diseases caused by alterations in glycosylation pathways	180:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	1	46	theme	Congenital	121:130	arg1	disorders					132:140	Congenital disorders	121:140	Congenital disorders of glycosylation (CDG)	121:163	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	1	46	theme	Congenital	121:130	arg1	group					171:175	a group	169:175	a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways	169:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	9	47	theme	background	1471:1480	arg1	understanding					1440:1452	our understanding	1436:1452	our understanding of the molecular background of SLC35A1-CDG cases	1436:1501	The results of this study improve our understanding of the molecular background of SLC35A1-CDG cases.					
33396746	7	48	theme	CST-deficient	1171:1183	arg1	cells					1185:1189	CST-deficient cells	1171:1189	CST-deficient cells	1171:1189	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	7	49	theme	Single	1087:1092	arg1	mutants					1117:1123	Single Q101H, T156R and E196K mutants	1087:1123	Single Q101H, T156R and E196K mutants	1087:1123	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	7	50	theme	mutation	1246:1253	arg1	effect					1212:1217	the deleterious effect	1196:1217	the deleterious effect of a single T156R or E196K mutation on the CST functionality	1196:1278	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	2	51	theme	CMP-sialic	342:351	arg1	CST					371:373	CST	371:373	CST	371:373	Five patients bearing CDG-causing mutations in the SLC35A1 gene encoding the CMP-sialic acid transporter (CST) have been reported to date.					
33396746	2	51	theme	CMP-sialic	342:351	arg1	transporter					358:368	the CMP-sialic acid transporter	338:368	the CMP-sialic acid transporter (CST)	338:374	Five patients bearing CDG-causing mutations in the SLC35A1 gene encoding the CMP-sialic acid transporter (CST) have been reported to date.					
33396746	4	52	theme	mutations	681:689	arg1	effects					663:669	The effects	659:669	The effects of single mutations	659:689	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	4	52	theme	mutations	681:689	arg1	combination					699:709	the combination	695:709	the combination of T156R and E196K mutations	695:738	The effects of single mutations and the combination of T156R and E196K mutations on the CST's functionality was examined separately in CST-deficient HEK293T cells.					
33396746	3	53	theme	SLC35A1	460:466	arg1	gene					468:471	the SLC35A1 gene	456:471	the SLC35A1 gene	456:471	In this study we examined how specific mutations in the SLC35A1 gene affect the protein's properties in two previously described SLC35A1-CDG cases: one caused by a substitution (Q101H) and another involving a compound heterozygous mutation (T156R/E196K).					
33396746	5	54	theme	proper	913:918	arg1	localization					920:931	the protein's proper localization	899:931	the protein's proper localization in the Golgi apparatus	899:954	As shown by microscopic studies, none of the CDG-causing mutations affected the protein's proper localization in the Golgi apparatus.					
33396746	5	55	theme	CDG-causing	868:878	arg1	mutations					880:888	the CDG-causing mutations	864:888	the CDG-causing mutations	864:888	As shown by microscopic studies, none of the CDG-causing mutations affected the protein's proper localization in the Golgi apparatus.					
33396746	7	56	gly	restore	1148:1154	arg1	cells					1185:1189	CST-deficient cells	1171:1189	CST-deficient cells	1171:1189	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	1	57	theme	glycosylation	145:157	arg1	diseases					207:214	rare genetic and metabolic diseases	180:214	rare genetic and metabolic diseases caused by alterations in glycosylation pathways	180:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	1	57	theme	glycosylation	145:157	arg1	disorders					132:140	Congenital disorders	121:140	Congenital disorders of glycosylation (CDG)	121:163	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	1	57	theme	glycosylation	145:157	arg1	group					171:175	a group	169:175	a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways	169:262	Congenital disorders of glycosylation (CDG) are a group of rare genetic and metabolic diseases caused by alterations in glycosylation pathways.					
33396746	2	58	from	mutations	299:307	arg1	gene					324:327	the SLC35A1 gene	312:327	the SLC35A1 gene encoding the CMP-sialic acid transporter (CST)	312:374	Five patients bearing CDG-causing mutations in the SLC35A1 gene encoding the CMP-sialic acid transporter (CST) have been reported to date.					
33396746	9	59	theme	molecular	1461:1469	arg1	background					1471:1480	the molecular background	1457:1480	the molecular background of SLC35A1-CDG cases	1457:1501	The results of this study improve our understanding of the molecular background of SLC35A1-CDG cases.					
33396746	7	60	theme	deleterious	1200:1210	arg1	effect					1212:1217	the deleterious effect	1196:1217	the deleterious effect of a single T156R or E196K mutation on the CST functionality	1196:1278	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	6	61	theme	structural	1016:1025	arg1	lectins					1007:1013	lectins	1007:1013	lectins	1007:1013	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	6	61	theme	structural	1016:1025	arg1	assignment					1027:1036	structural assignment	1016:1036	structural assignment of N- and O-glycans	1016:1056	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	6	62	theme	N-	1041:1042	arg1	lectins					1007:1013	lectins	1007:1013	lectins	1007:1013	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	6	62	theme	N-	1041:1042	arg1	assignment					1027:1036	structural assignment	1016:1036	structural assignment of N- and O-glycans	1016:1056	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	6	62	theme	N-	1041:1042	arg1	analysis					1062:1069	analysis	1062:1069	analysis of glycolipids	1062:1084	Cellular glycophenotypes were characterized using lectins, structural assignment of N- and O-glycans and analysis of glycolipids.					
33396746	7	63	theme	single	1224:1229	arg1	T156R					1231:1235	a single T156R or E196K mutation	1222:1253	T156R	1231:1235	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	5	64	from	localization	920:931	arg1	apparatus					946:954	the Golgi apparatus	936:954	the Golgi apparatus	936:954	As shown by microscopic studies, none of the CDG-causing mutations affected the protein's proper localization in the Golgi apparatus.					
33396746	0	65	theme	SLC35A1	66:72	arg1	Gene					74:77	the SLC35A1 Gene	62:77	the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter	62:118	Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter.					
33396746	7	66	theme	T156R	1231:1235	arg1	effect					1212:1217	the deleterious effect	1196:1217	the deleterious effect of a single T156R or E196K mutation on the CST functionality	1196:1278	Single Q101H, T156R and E196K mutants were able to partially restore sialylation in CST-deficient cells, and the deleterious effect of a single T156R or E196K mutation on the CST functionality was strongly enhanced upon their combination.					
33396746	8	67	theme	CST	1373:1375	arg1	variants					1377:1384	CST variants	1373:1384	CST variants	1373:1384	We also revealed differences in the ability of CST variants to form dimers.					
34876606	4	0	theme	S-614G	827:832	arg1	SARS-CoV-2					835:844	variant (S-614G) SARS-CoV-2	818:844	variant (S-614G) SARS-CoV-2	818:844	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	3	1	theme	first	486:490	arg1	variant					509:515	The first major SARS-CoV-2 variant	482:515	The first major SARS-CoV-2 variant	482:515	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	8	2	theme	spike	1355:1359	arg1	head					1361:1364	the spike head	1351:1364	the spike head	1351:1364	All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.					
34876606	0	3	theme	ancestral	70:78	arg1	protein					80:86	its ancestral protein	66:86	its ancestral protein	66:86	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.					
34876606	4	4	theme	wild	796:799	arg1	S-614D					807:812	S-614D	807:812	S-614D	807:812	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	4	4	theme	wild	796:799	arg1	type					801:804	wild type	796:804	wild type (S-614D)	796:813	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	4	5	theme	variant	818:824	arg1	SARS-CoV-2					835:844	variant (S-614G) SARS-CoV-2	818:844	variant (S-614G) SARS-CoV-2	818:844	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	6	6	theme	relative	1065:1072	arg1	abundance					1074:1082	the relative abundance	1061:1082	the relative abundance of complex-type glycans (up to 45%)	1061:1118	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	3	7	theme	major	492:496	arg1	variant					509:515	The first major SARS-CoV-2 variant	482:515	The first major SARS-CoV-2 variant	482:515	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	6	8	dep	33	1167:1168	arg1	to					1164:1165	to	1164:1165	to	1164:1165	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	3	9	theme	altered	599:605	arg1	conformation					607:618	altered conformation	599:618	altered conformation	599:618	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	2	10	gly	glycosylated	383:394	arg1	glycosylated					383:394	glycosylated	383:394	glycosylated	383:394	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	2	10	gly	glycosylated	383:394	arg1	protein					248:254	The spike protein	238:254	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19,	238:370	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	8	11	theme	altered	1326:1332	arg1	patterns					1334:1341	altered patterns	1326:1341	altered patterns	1326:1341	All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.					
34876606	2	12	theme	acute	285:289	arg1	virus					344:348	the virus	340:348	the virus that causes COVID-19	340:369	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	2	12	theme	acute	285:289	arg1	coronavirus					312:322	the severe acute respiratory syndrome coronavirus 2	274:324	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	274:337	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	2	12	theme	acute	285:289	arg1	SARS-CoV-2					327:336	SARS-CoV-2	327:336	SARS-CoV-2	327:336	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	3	13	from	substitution	533:544	arg1	S-D614G					560:566	S-D614G	560:566	S-D614G	560:566	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	3	13	from	substitution	533:544	arg1	spike					553:557	the spike	549:557	the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission	549:685	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	8	14	gly	glycosylation	1301:1313	arg2	sites					1315:1319	All the glycosylation sites	1293:1319	All the glycosylation sites with altered patterns	1293:1341	All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.					
34876606	6	15	from	decrease	1049:1056	arg1	glycans					1152:1158	oligomannose glycans	1139:1158	oligomannose glycans (up to 33%) on all altered sequons	1139:1193	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	15	from	decrease	1049:1056	arg1	abundance					1074:1082	the relative abundance	1061:1082	the relative abundance of complex-type glycans (up to 45%)	1061:1118	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	15	from	decrease	1049:1056	arg1	sequons					1187:1193	all altered sequons	1175:1193	all altered sequons	1175:1193	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	15	from	decrease	1049:1056	arg1	%					1169:1169	up to 33%	1161:1169	up to 33%	1161:1169	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	2	16	theme	severe	278:283	arg1	virus					344:348	the virus	340:348	the virus that causes COVID-19	340:369	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	2	16	theme	severe	278:283	arg1	coronavirus					312:322	the severe acute respiratory syndrome coronavirus 2	274:324	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	274:337	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	2	16	theme	severe	278:283	arg1	SARS-CoV-2					327:336	SARS-CoV-2	327:336	SARS-CoV-2	327:336	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	0	17	theme	mass	114:117	arg1	spectrometry					119:130	advanced mass spectrometry	105:130	advanced mass spectrometry	105:130	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.					
34876606	6	18	theme	altered	1179:1185	arg1	sequons					1187:1193	all altered sequons	1175:1193	all altered sequons	1175:1193	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	5	19	theme	N-glycosylation	936:950	arg1	sequons					952:958	the N-glycosylation sequons	932:958	the N-glycosylation sequons	932:958	The data showed that half of the N-glycosylation sequons changed their distribution of glycans in the S-614G variant.					
34876606	7	20	theme	overall	1236:1242	arg1	complexity					1244:1253	the overall complexity	1232:1253	the overall complexity of the total N-glycosylation profile	1232:1290	These changes led to a reduction in the overall complexity of the total N-glycosylation profile.					
34876606	0	21	theme	advanced	105:112	arg1	spectrometry					119:130	advanced mass spectrometry	105:130	advanced mass spectrometry	105:130	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.					
34876606	5	22	gly	N-glycosylation	936:950	arg2	sequons					952:958	the N-glycosylation sequons	932:958	the N-glycosylation sequons	932:958	The data showed that half of the N-glycosylation sequons changed their distribution of glycans in the S-614G variant.					
34876606	8	23	from	glycosylation	1376:1388	arg1	stalk					1412:1416	the stalk	1408:1416	the stalk	1408:1416	All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.					
34876606	5	24	theme	sequons	952:958	arg1	half					924:927	half	924:927	half of the N-glycosylation sequons	924:958	The data showed that half of the N-glycosylation sequons changed their distribution of glycans in the S-614G variant.					
34876606	6	25	from	increase	1127:1134	arg1	glycans					1152:1158	oligomannose glycans	1139:1158	oligomannose glycans (up to 33%) on all altered sequons	1139:1193	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	25	from	increase	1127:1134	arg1	abundance					1074:1082	the relative abundance	1061:1082	the relative abundance of complex-type glycans (up to 45%)	1061:1118	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	25	from	increase	1127:1134	arg1	sequons					1187:1193	all altered sequons	1175:1193	all altered sequons	1175:1193	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	25	from	increase	1127:1134	arg1	%					1169:1169	up to 33%	1161:1169	up to 33%	1161:1169	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	2	26	theme	diagnostic	464:473	arg1	tests					475:479	diagnostic tests	464:479	diagnostic tests	464:479	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	4	27	gly	N-glycosylation	773:787	arg1	glycoproteins					852:864	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins	792:864	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions	792:900	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	4	28	theme	spike	846:850	arg1	glycoproteins					852:864	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins	792:864	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions	792:900	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	0	29	theme	N-glycosylation	0:14	arg1	profiles					16:23	N-glycosylation profiles	0:23	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant	0:60	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.					
34876606	5	30	theme	glycans	990:996	arg1	distribution					974:985	their distribution	968:985	their distribution of glycans	968:996	The data showed that half of the N-glycosylation sequons changed their distribution of glycans in the S-614G variant.					
34876606	2	31	theme	coronavirus	312:322	arg1	surface					263:269	the surface	259:269	the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19,	259:370	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	7	32	theme	N-glycosylation	1268:1282	arg1	profile					1284:1290	the total N-glycosylation profile	1258:1290	the total N-glycosylation profile	1258:1290	These changes led to a reduction in the overall complexity of the total N-glycosylation profile.					
34876606	6	33	dep	45	1115:1116	arg1	to					1112:1113	to	1112:1113	to	1112:1113	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	34	from	glycans	1152:1158	arg1	sequons					1187:1193	all altered sequons	1175:1193	all altered sequons	1175:1193	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	8	35	theme	S-614D	1456:1461	arg1	proteins					1463:1470	S-614D proteins	1456:1470	S-614D proteins	1456:1470	All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.					
34876606	4	36	theme	type	801:804	arg1	glycoproteins					852:864	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins	792:864	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions	792:900	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	2	37	theme	syndrome	303:310	arg1	virus					344:348	the virus	340:348	the virus that causes COVID-19	340:369	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	2	37	theme	syndrome	303:310	arg1	coronavirus					312:322	the severe acute respiratory syndrome coronavirus 2	274:324	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	274:337	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	2	37	theme	syndrome	303:310	arg1	SARS-CoV-2					327:336	SARS-CoV-2	327:336	SARS-CoV-2	327:336	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	0	38	gly	N-glycosylation	0:14	arg1	mutant					55:60	the SARS-CoV-2 spike D614G mutant	28:60	the SARS-CoV-2 spike D614G mutant	28:60	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.					
34876606	2	39	theme	major	404:408	arg1	target					410:415	the major target	400:415	the major target for developing vaccines, therapeutic drugs and diagnostic tests	400:479	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	8	40	with	sites	1315:1319	arg1	patterns					1334:1341	altered patterns	1326:1341	altered patterns	1326:1341	All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.					
34876606	4	41	theme	identical	881:889	arg1	conditions					891:900	identical conditions	881:900	identical conditions	881:900	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	3	42	theme	enhanced	621:628	arg1	binding					635:641	enhanced ACE2 binding	621:641	enhanced ACE2 binding	621:641	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	2	43	theme	respiratory	291:301	arg1	virus					344:348	the virus	340:348	the virus that causes COVID-19	340:369	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	2	43	theme	respiratory	291:301	arg1	coronavirus					312:322	the severe acute respiratory syndrome coronavirus 2	274:324	the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	274:337	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	2	43	theme	respiratory	291:301	arg1	SARS-CoV-2					327:336	SARS-CoV-2	327:336	SARS-CoV-2	327:336	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	1	44	theme	membrane	206:213	arg1	function					194:201	function	194:201	function	194:201	N-glycosylation plays an important role in the structure and function of membrane and secreted proteins.					
34876606	1	44	theme	membrane	206:213	arg1	structure					180:188	structure	180:188	structure	180:188	N-glycosylation plays an important role in the structure and function of membrane and secreted proteins.					
34876606	0	45	theme	spike	43:47	arg1	mutant					55:60	the SARS-CoV-2 spike D614G mutant	28:60	the SARS-CoV-2 spike D614G mutant	28:60	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.					
34876606	6	46	theme	glycans	1100:1106	arg1	abundance					1074:1082	the relative abundance	1061:1082	the relative abundance of complex-type glycans (up to 45%)	1061:1118	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	3	47	theme	ACE2	630:633	arg1	binding					635:641	enhanced ACE2 binding	621:641	enhanced ACE2 binding	621:641	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	2	48	theme	spike	242:246	arg1	glycosylated					383:394	glycosylated	383:394	glycosylated	383:394	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	2	48	theme	spike	242:246	arg1	protein					248:254	The spike protein	238:254	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19,	238:370	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	0	49	theme	SARS-CoV-2	32:41	arg1	mutant					55:60	the SARS-CoV-2 spike D614G mutant	28:60	the SARS-CoV-2 spike D614G mutant	28:60	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.					
34876606	8	50	theme	glycosylation	1301:1313	arg1	sites					1315:1319	All the glycosylation sites	1293:1319	All the glycosylation sites with altered patterns	1293:1341	All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.					
34876606	1	51	theme	secreted	219:226	arg1	proteins					228:235	secreted proteins	219:235	secreted proteins	219:235	N-glycosylation plays an important role in the structure and function of membrane and secreted proteins.					
34876606	0	52	theme	mutant	55:60	arg1	protein					80:86	its ancestral protein	66:86	its ancestral protein	66:86	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.					
34876606	0	52	theme	mutant	55:60	arg1	profiles					16:23	N-glycosylation profiles	0:23	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant	0:60	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.					
34876606	3	53	contain	carries	517:523	arg2	substitution					533:544	a D614G substitution	525:544	a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission	525:685	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	3	53	contain	carries	517:523	arg1	variant					509:515	The first major SARS-CoV-2 variant	482:515	The first major SARS-CoV-2 variant	482:515	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	4	54	theme	glycoproteins	852:864	arg1	N-glycosylation					773:787	the N-glycosylation	769:787	the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions	769:900	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	7	55	theme	profile	1284:1290	arg1	complexity					1244:1253	the overall complexity	1232:1253	the overall complexity of the total N-glycosylation profile	1232:1290	These changes led to a reduction in the overall complexity of the total N-glycosylation profile.					
34876606	0	56	theme	D614G	49:53	arg1	mutant					55:60	the SARS-CoV-2 spike D614G mutant	28:60	the SARS-CoV-2 spike D614G mutant	28:60	N-glycosylation profiles of the SARS-CoV-2 spike D614G mutant and its ancestral protein characterized by advanced mass spectrometry.					
34876606	4	57	gly	glycoproteins	852:864	arg1	glycoproteins					852:864	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins	792:864	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions	792:900	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	5	58	theme	S-614G	1005:1010	arg1	variant					1012:1018	the S-614G variant	1001:1018	the S-614G variant	1001:1018	The data showed that half of the N-glycosylation sequons changed their distribution of glycans in the S-614G variant.					
34876606	1	59	dep	structure	180:188	arg1	the					176:178	the	176:178	the	176:178	N-glycosylation plays an important role in the structure and function of membrane and secreted proteins.					
34876606	4	60	theme	SARS-CoV-2	835:844	arg1	glycoproteins					852:864	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins	792:864	the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions	792:900	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	3	61	theme	increased	648:656	arg1	infectivity					658:668	infectivity	658:668	infectivity	658:668	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	7	62	theme	total	1262:1266	arg1	profile					1284:1290	the total N-glycosylation profile	1258:1290	the total N-glycosylation profile	1258:1290	These changes led to a reduction in the overall complexity of the total N-glycosylation profile.					
34876606	7	63	from	reduction	1219:1227	arg1	complexity					1244:1253	the overall complexity	1232:1253	the overall complexity of the total N-glycosylation profile	1232:1290	These changes led to a reduction in the overall complexity of the total N-glycosylation profile.					
34876606	6	64	theme	complex-type	1087:1098	arg1	%					1117:1117	up to 45%	1109:1117	up to 45%	1109:1117	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	64	theme	complex-type	1087:1098	arg1	glycans					1100:1106	complex-type glycans	1087:1106	complex-type glycans (up to 45%)	1087:1118	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	4	65	theme	spectrometry	717:728	arg1	techniques					730:739	mass spectrometry techniques	712:739	mass spectrometry techniques	712:739	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	1	66	theme	proteins	228:235	arg1	function					194:201	function	194:201	function	194:201	N-glycosylation plays an important role in the structure and function of membrane and secreted proteins.					
34876606	1	66	theme	proteins	228:235	arg1	structure					180:188	structure	180:188	structure	180:188	N-glycosylation plays an important role in the structure and function of membrane and secreted proteins.					
34876606	8	67	gly	glycosylation	1376:1388	arg1	stalk					1412:1416	the stalk	1408:1416	the stalk	1408:1416	All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.					
34876606	8	67	gly	glycosylation	1376:1388	arg1	sites					1399:1403	three sites	1393:1403	three sites	1393:1403	All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.					
34876606	8	68	theme	sites	1399:1403	arg1	glycosylation					1376:1388	the glycosylation	1372:1388	the glycosylation of three sites in the stalk	1372:1416	All the glycosylation sites with altered patterns were in the spike head while the glycosylation of three sites in the stalk remained unchanged between S-614G and S-614D proteins.					
34876606	3	69	theme	D614G	527:531	arg1	substitution					533:544	a D614G substitution	525:544	a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission	525:685	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	4	70	theme	mass	712:715	arg1	techniques					730:739	mass spectrometry techniques	712:739	mass spectrometry techniques	712:739	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	2	71	from	protein	248:254	arg1	surface					263:269	the surface	259:269	the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19,	259:370	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	6	72	from	sequons	1187:1193	arg1	increase					1127:1134	an increase	1124:1134	an increase in oligomannose glycans (up to 33%) on all altered sequons	1124:1193	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	72	from	sequons	1187:1193	arg1	decrease					1049:1056	a decrease	1047:1056	a decrease in the relative abundance of complex-type glycans (up to 45%)	1047:1118	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	4	73	used	used	707:710	arg2	we					704:705	we	704:705	we	704:705	In this report, we used mass spectrometry techniques to characterize and compare the N-glycosylation of the wild type (S-614D) or variant (S-614G) SARS-CoV-2 spike glycoproteins prepared under identical conditions.					
34876606	6	74	theme	oligomannose	1139:1150	arg1	glycans					1152:1158	oligomannose glycans	1139:1158	oligomannose glycans (up to 33%) on all altered sequons	1139:1193	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	74	theme	oligomannose	1139:1150	arg1	%					1169:1169	up to 33%	1161:1169	up to 33%	1161:1169	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	6	75	theme	S-614G	1025:1030	arg1	variant					1032:1038	The S-614G variant	1021:1038	The S-614G variant	1021:1038	The S-614G variant showed a decrease in the relative abundance of complex-type glycans (up to 45%) and an increase in oligomannose glycans (up to 33%) on all altered sequons.					
34876606	3	76	theme	SARS-CoV-2	498:507	arg1	variant					509:515	The first major SARS-CoV-2 variant	482:515	The first major SARS-CoV-2 variant	482:515	The first major SARS-CoV-2 variant carries a D614G substitution in the spike (S-D614G) that has been associated with altered conformation, enhanced ACE2 binding, and increased infectivity and transmission.					
34876606	2	77	theme	therapeutic	442:452	arg1	drugs					454:458	therapeutic drugs	442:458	therapeutic drugs	442:458	The spike protein on the surface of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, is heavily glycosylated and the major target for developing vaccines, therapeutic drugs and diagnostic tests.					
34876606	1	78	theme	important	158:166	arg1	role					168:171	an important role	155:171	an important role	155:171	N-glycosylation plays an important role in the structure and function of membrane and secreted proteins.					
31883005	9	0	theme	N-linked	1344:1351	arg1	glycosylation					1353:1365	N-linked glycosylation	1344:1365	N-linked glycosylation	1344:1365	Salacinol inhibited the processing glucosidase I/II, which are involved in the initial stage of N-linked glycosylation.					
31883005	5	1	theme	unique	657:662	arg1	structure					705:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	10	2	theme	high	1413:1416	arg1	hydrophilicity					1418:1431	high hydrophilicity	1413:1431	high hydrophilicity	1413:1431	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
31883005	6	3	contain	possesses	782:790	arg1	component					753:761	the salacinol component	739:761	the salacinol component of Salacia extract	739:780	Here, we observed that the salacinol component of Salacia extract possesses anti-melanogenic activity in comparison to various existing whitening agents.					
31883005	6	3	contain	possesses	782:790	arg2	activity					809:816	anti-melanogenic activity	792:816	anti-melanogenic activity in comparison to various existing whitening agents	792:867	Here, we observed that the salacinol component of Salacia extract possesses anti-melanogenic activity in comparison to various existing whitening agents.					
31883005	5	4	contain	has	653:655	arg1	Salacinol					600:608	Salacinol	600:608	Salacinol	600:608	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	5	4	contain	has	653:655	arg1	ingredient					622:631	the active ingredient	611:631	the active ingredient in Salacia extract	611:650	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	5	4	contain	has	653:655	arg2	structure					705:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	8	5	theme	due	1190:1192	arg1	form					1185:1188	the mature tyrosinase form	1163:1188	the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing	1163:1245	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	9	6	theme	glycosylation	1353:1365	arg1	stage					1335:1339	the initial stage	1323:1339	the initial stage of N-linked glycosylation	1323:1365	Salacinol inhibited the processing glucosidase I/II, which are involved in the initial stage of N-linked glycosylation.					
31883005	5	7	theme	thiosugar	664:672	arg1	structure					705:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	6	8	theme	whitening	852:860	arg1	agents					862:867	various existing whitening agents	835:867	various existing whitening agents	835:867	Here, we observed that the salacinol component of Salacia extract possesses anti-melanogenic activity in comparison to various existing whitening agents.					
31883005	7	9	theme	whitening	1018:1026	arg1	agents					1028:1033	whitening agents	1018:1033	whitening agents	1018:1033	Although the anti-melanogenic mechanism of salacinol is presumably medicated by inhibition of tyrosinase activity, which is often found in existing whitening agents, salacinol did not inhibit tyrosinase activity in vitro.					
31883005	4	10	theme	α-glucosidase	585:597	arg1	activity					573:580	the activity	569:580	the activity of α-glucosidase	569:597	Salacia extract reportedly contains components that inhibit the activity of α-glucosidase.					
31883005	4	11	theme	Salacia	509:515	arg1	extract					517:523	Salacia extract	509:523	Salacia extract	509:523	Salacia extract reportedly contains components that inhibit the activity of α-glucosidase.					
31883005	5	12	theme	sulphonium	674:683	arg1	structure					705:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	5	13	theme	active	615:620	arg1	Salacinol					600:608	Salacinol	600:608	Salacinol	600:608	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	5	13	theme	active	615:620	arg1	ingredient					622:631	the active ingredient	611:631	the active ingredient in Salacia extract	611:650	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	5	14	theme	sulphate	685:692	arg1	structure					705:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	6	15	theme	various	835:841	arg1	agents					862:867	various existing whitening agents	835:867	various existing whitening agents	835:867	Here, we observed that the salacinol component of Salacia extract possesses anti-melanogenic activity in comparison to various existing whitening agents.					
31883005	8	16	from	decrease	1151:1158	arg1	form					1185:1188	the mature tyrosinase form	1163:1188	the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing	1163:1245	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	8	17	theme	oligosaccharide	1220:1234	arg1	processing					1236:1245	N-linked oligosaccharide processing	1211:1245	N-linked oligosaccharide processing	1211:1245	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	10	18	theme	whitening	1481:1489	arg1	agents					1491:1496	whitening agents	1481:1496	whitening agents aimed for external application on skin	1481:1535	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
31883005	5	19	theme	Salacia	636:642	arg1	extract					644:650	Salacia extract	636:650	Salacia extract	636:650	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	7	20	theme	salacinol	913:921	arg1	mechanism					900:908	the anti-melanogenic mechanism	879:908	the anti-melanogenic mechanism of salacinol	879:921	Although the anti-melanogenic mechanism of salacinol is presumably medicated by inhibition of tyrosinase activity, which is often found in existing whitening agents, salacinol did not inhibit tyrosinase activity in vitro.					
31883005	8	21	theme	N-linked	1211:1218	arg1	processing					1236:1245	N-linked oligosaccharide processing	1211:1245	N-linked oligosaccharide processing	1211:1245	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	4	22	contain	contains	536:543	arg2	components					545:554	components	545:554	components that inhibit the activity of α-glucosidase	545:597	Salacia extract reportedly contains components that inhibit the activity of α-glucosidase.					
31883005	4	22	contain	contains	536:543	arg1	extract					517:523	Salacia extract	509:523	Salacia extract	509:523	Salacia extract reportedly contains components that inhibit the activity of α-glucosidase.					
31883005	7	23	theme	anti-melanogenic	883:898	arg1	mechanism					900:908	the anti-melanogenic mechanism	879:908	the anti-melanogenic mechanism of salacinol	879:921	Although the anti-melanogenic mechanism of salacinol is presumably medicated by inhibition of tyrosinase activity, which is often found in existing whitening agents, salacinol did not inhibit tyrosinase activity in vitro.					
31883005	2	24	theme	tyrosinase	413:422	arg1	maturation					399:408	the maturation	395:408	the maturation of tyrosinase	395:422	Controlling the rate-limiting activity of tyrosinase is most effective for suppressing excessive melanin formation and accordingly recent research has focused on the maturation of tyrosinase.					
31883005	8	25	theme	state	1122:1126	arg1	Analysis					1092:1099	Analysis	1092:1099	Analysis of the intracellular state of tyrosinase	1092:1140	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	9	26	theme	processing	1272:1281	arg1	I/II					1295:1298	the processing glucosidase I/II	1268:1298	the processing glucosidase I/II	1268:1298	Salacinol inhibited the processing glucosidase I/II, which are involved in the initial stage of N-linked glycosylation.					
31883005	0	27	theme	Anti-melanogenic	0:15	arg1	activity					17:24	Anti-melanogenic activity	0:24	Anti-melanogenic activity of salacinol by inhibition of tyrosinase oligosaccharide processing	0:92	Anti-melanogenic activity of salacinol by inhibition of tyrosinase oligosaccharide processing.					
31883005	8	28	theme	intracellular	1108:1120	arg1	state					1122:1126	the intracellular state	1104:1126	the intracellular state of tyrosinase	1104:1140	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	9	29	theme	glucosidase	1283:1293	arg1	I/II					1295:1298	the processing glucosidase I/II	1268:1298	the processing glucosidase I/II	1268:1298	Salacinol inhibited the processing glucosidase I/II, which are involved in the initial stage of N-linked glycosylation.					
31883005	3	30	used	used	462:465	arg2	plant					446:450	a medicinal plant	434:450	a medicinal plant	434:450	Salacia, a medicinal plant, has been used to treat diabetes in India and Sri Lanka.					
31883005	3	30	used	used	462:465	arg2	Salacia					425:431	Salacia	425:431	Salacia	425:431	Salacia, a medicinal plant, has been used to treat diabetes in India and Sri Lanka.					
31883005	10	31	theme	external	1508:1515	arg1	application					1517:1527	external application	1508:1527	external application on skin	1508:1535	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
31883005	0	32	theme	salacinol	29:37	arg1	activity					17:24	Anti-melanogenic activity	0:24	Anti-melanogenic activity of salacinol by inhibition of tyrosinase oligosaccharide processing	0:92	Anti-melanogenic activity of salacinol by inhibition of tyrosinase oligosaccharide processing.					
31883005	8	33	theme	tyrosinase	1131:1140	arg1	state					1122:1126	the intracellular state	1104:1126	the intracellular state of tyrosinase	1104:1140	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	6	34	theme	anti-melanogenic	792:807	arg1	activity					809:816	anti-melanogenic activity	792:816	anti-melanogenic activity in comparison to various existing whitening agents	792:867	Here, we observed that the salacinol component of Salacia extract possesses anti-melanogenic activity in comparison to various existing whitening agents.					
31883005	2	35	theme	excessive	320:328	arg1	formation					338:346	excessive melanin formation	320:346	excessive melanin formation	320:346	Controlling the rate-limiting activity of tyrosinase is most effective for suppressing excessive melanin formation and accordingly recent research has focused on the maturation of tyrosinase.					
31883005	10	36	theme	candidate	1459:1467	arg1	compound					1469:1476	a promising candidate compound	1447:1476	a promising candidate compound in whitening agents aimed for external application on skin	1447:1535	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
31883005	10	36	theme	candidate	1459:1467	arg1	salacinol					1434:1442	salacinol	1434:1442	salacinol	1434:1442	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
31883005	6	37	from	activity	809:816	arg1	comparison					821:830	comparison	821:830	comparison to various existing whitening agents	821:867	Here, we observed that the salacinol component of Salacia extract possesses anti-melanogenic activity in comparison to various existing whitening agents.					
31883005	2	38	theme	melanin	330:336	arg1	formation					338:346	excessive melanin formation	320:346	excessive melanin formation	320:346	Controlling the rate-limiting activity of tyrosinase is most effective for suppressing excessive melanin formation and accordingly recent research has focused on the maturation of tyrosinase.					
31883005	10	39	from	application	1517:1527	arg1	skin					1532:1535	skin	1532:1535	skin	1532:1535	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
31883005	10	40	theme	low	1392:1394	arg1	cytotoxicity					1396:1407	low cytotoxicity	1392:1407	low cytotoxicity	1392:1407	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
31883005	5	41	from	ingredient	622:631	arg1	extract					644:650	Salacia extract	636:650	Salacia extract	636:650	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	3	42	theme	Sri	498:500	arg1	Lanka					502:506	Sri Lanka	498:506	Sri Lanka	498:506	Salacia, a medicinal plant, has been used to treat diabetes in India and Sri Lanka.					
31883005	9	43	link	N-linked	1344:1351	arg1	glycosylation					1353:1365	N-linked glycosylation	1344:1365	N-linked glycosylation	1344:1365	Salacinol inhibited the processing glucosidase I/II, which are involved in the initial stage of N-linked glycosylation.					
31883005	3	44	theme	medicinal	436:444	arg1	plant					446:450	a medicinal plant	434:450	a medicinal plant	434:450	Salacia, a medicinal plant, has been used to treat diabetes in India and Sri Lanka.					
31883005	3	44	theme	medicinal	436:444	arg1	Salacia					425:431	Salacia	425:431	Salacia	425:431	Salacia, a medicinal plant, has been used to treat diabetes in India and Sri Lanka.					
31883005	7	45	theme	tyrosinase	1062:1071	arg1	activity					1073:1080	tyrosinase activity	1062:1080	tyrosinase activity	1062:1080	Although the anti-melanogenic mechanism of salacinol is presumably medicated by inhibition of tyrosinase activity, which is often found in existing whitening agents, salacinol did not inhibit tyrosinase activity in vitro.					
31883005	1	46	theme	many	220:223	arg1	people					225:230	many people	220:230	many people	220:230	Hyperpigmentation that manifests through melasma and solar lentigo (age spots), although mostly harmless for health, bothers many people.					
31883005	0	47	theme	tyrosinase	56:65	arg1	processing					83:92	tyrosinase oligosaccharide processing	56:92	tyrosinase oligosaccharide processing	56:92	Anti-melanogenic activity of salacinol by inhibition of tyrosinase oligosaccharide processing.					
31883005	5	48	theme	inner	694:698	arg1	structure					705:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	8	49	theme	tyrosinase	1174:1183	arg1	form					1185:1188	the mature tyrosinase form	1163:1188	the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing	1163:1245	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	6	50	theme	extract	774:780	arg1	component					753:761	the salacinol component	739:761	the salacinol component of Salacia extract	739:780	Here, we observed that the salacinol component of Salacia extract possesses anti-melanogenic activity in comparison to various existing whitening agents.					
31883005	10	51	theme	promising	1449:1457	arg1	compound					1469:1476	a promising candidate compound	1447:1476	a promising candidate compound in whitening agents aimed for external application on skin	1447:1535	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
31883005	10	51	theme	promising	1449:1457	arg1	salacinol					1434:1442	salacinol	1434:1442	salacinol	1434:1442	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
31883005	5	52	theme	salt	700:703	arg1	structure					705:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	unique thiosugar sulphonium sulphate inner salt structure	657:713	Salacinol, the active ingredient in Salacia extract, has unique thiosugar sulphonium sulphate inner salt structure.					
31883005	1	53	dep	mostly	184:189	arg1	harmless					191:198	harmless	191:198	harmless	191:198	Hyperpigmentation that manifests through melasma and solar lentigo (age spots), although mostly harmless for health, bothers many people.					
31883005	6	54	theme	Salacia	766:772	arg1	extract					774:780	Salacia extract	766:780	Salacia extract	766:780	Here, we observed that the salacinol component of Salacia extract possesses anti-melanogenic activity in comparison to various existing whitening agents.					
31883005	2	55	theme	recent	364:369	arg1	research					371:378	recent research	364:378	recent research	364:378	Controlling the rate-limiting activity of tyrosinase is most effective for suppressing excessive melanin formation and accordingly recent research has focused on the maturation of tyrosinase.					
31883005	7	56	theme	tyrosinase	964:973	arg1	activity					975:982	tyrosinase activity	964:982	tyrosinase activity	964:982	Although the anti-melanogenic mechanism of salacinol is presumably medicated by inhibition of tyrosinase activity, which is often found in existing whitening agents, salacinol did not inhibit tyrosinase activity in vitro.					
31883005	8	57	theme	mature	1167:1172	arg1	form					1185:1188	the mature tyrosinase form	1163:1188	the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing	1163:1245	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	2	58	theme	tyrosinase	275:284	arg1	activity					263:270	the rate-limiting activity	245:270	the rate-limiting activity of tyrosinase	245:284	Controlling the rate-limiting activity of tyrosinase is most effective for suppressing excessive melanin formation and accordingly recent research has focused on the maturation of tyrosinase.					
31883005	6	59	theme	existing	843:850	arg1	agents					862:867	various existing whitening agents	835:867	various existing whitening agents	835:867	Here, we observed that the salacinol component of Salacia extract possesses anti-melanogenic activity in comparison to various existing whitening agents.					
31883005	7	60	theme	activity	975:982	arg1	inhibition					950:959	inhibition	950:959	inhibition	950:959	Although the anti-melanogenic mechanism of salacinol is presumably medicated by inhibition of tyrosinase activity, which is often found in existing whitening agents, salacinol did not inhibit tyrosinase activity in vitro.					
31883005	9	61	theme	initial	1327:1333	arg1	stage					1335:1339	the initial stage	1323:1339	the initial stage of N-linked glycosylation	1323:1365	Salacinol inhibited the processing glucosidase I/II, which are involved in the initial stage of N-linked glycosylation.					
31883005	8	62	theme	processing	1236:1245	arg1	inhibition					1197:1206	inhibition	1197:1206	inhibition of N-linked oligosaccharide processing	1197:1245	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	10	63	theme	high	1377:1380	arg1	activity					1382:1389	high activity	1377:1389	high activity	1377:1389	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
31883005	0	64	theme	processing	83:92	arg1	inhibition					42:51	inhibition	42:51	inhibition of tyrosinase oligosaccharide processing	42:92	Anti-melanogenic activity of salacinol by inhibition of tyrosinase oligosaccharide processing.					
31883005	6	65	theme	salacinol	743:751	arg1	component					753:761	the salacinol component	739:761	the salacinol component of Salacia extract	739:780	Here, we observed that the salacinol component of Salacia extract possesses anti-melanogenic activity in comparison to various existing whitening agents.					
31883005	1	66	theme	age	163:165	arg1	lentigo					154:160	lentigo	154:160	lentigo	154:160	Hyperpigmentation that manifests through melasma and solar lentigo (age spots), although mostly harmless for health, bothers many people.					
31883005	1	66	theme	age	163:165	arg1	spots					167:171	age spots	163:171	age spots	163:171	Hyperpigmentation that manifests through melasma and solar lentigo (age spots), although mostly harmless for health, bothers many people.					
31883005	8	67	link	N-linked	1211:1218	arg1	processing					1236:1245	N-linked oligosaccharide processing	1211:1245	N-linked oligosaccharide processing	1211:1245	Analysis of the intracellular state of tyrosinase showed a decrease in the mature tyrosinase form due to inhibition of N-linked oligosaccharide processing.					
31883005	0	68	theme	oligosaccharide	67:81	arg1	processing					83:92	tyrosinase oligosaccharide processing	56:92	tyrosinase oligosaccharide processing	56:92	Anti-melanogenic activity of salacinol by inhibition of tyrosinase oligosaccharide processing.					
31883005	2	69	theme	rate-limiting	249:261	arg1	activity					263:270	the rate-limiting activity	245:270	the rate-limiting activity of tyrosinase	245:284	Controlling the rate-limiting activity of tyrosinase is most effective for suppressing excessive melanin formation and accordingly recent research has focused on the maturation of tyrosinase.					
31883005	10	70	from	compound	1469:1476	arg1	agents					1491:1496	whitening agents	1481:1496	whitening agents aimed for external application on skin	1481:1535	Owing to high activity, low cytotoxicity and high hydrophilicity, salacinol is a promising candidate compound in whitening agents aimed for external application on skin.					
33534538	7	0	used	utilized	1088:1095	arg2	databases					1019:1027	simple databases	1012:1027	simple databases containing only a single lipid form per GSL species	1012:1079	Furthermore, simple databases containing only a single lipid form per GSL species can be utilized to identify multiple GSL lipid forms.					
33534538	7	1	theme	single	1047:1052	arg1	form					1060:1063	only a single lipid form	1040:1063	only a single lipid form	1040:1063	Furthermore, simple databases containing only a single lipid form per GSL species can be utilized to identify multiple GSL lipid forms.					
33534538	4	2	theme	first	514:518	arg1	stage					520:524	the first stage	510:524	the first stage	510:524	In the first stage, carbohydrate fragments, which contain only glycans and thus are conserved within a GSL species, are directly matched to yield a species identification.					
33534538	6	3	theme	whole	958:962	arg1	spectrum					964:971	the whole spectrum	954:971	the whole spectrum for GSL characterization	954:996	This new strategy uses the whole spectrum for GSL characterization.					
33534538	5	4	theme	glycolipid	700:709	arg1	fragments					711:719	glycolipid fragments	700:719	glycolipid fragments	700:719	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	0	5	theme	LC-MS/MS	99:106	arg1	Spectra					108:114	LC-MS/MS Spectra	99:114	LC-MS/MS Spectra	99:114	Characterization of Glycosphingolipids and Their Diverse Lipid Forms through Two-Stage Matching of LC-MS/MS Spectra.					
33534538	7	6	theme	multiple	1109:1116	arg1	forms					1128:1132	multiple GSL lipid forms	1109:1132	multiple GSL lipid forms	1109:1132	Furthermore, simple databases containing only a single lipid form per GSL species can be utilized to identify multiple GSL lipid forms.					
33534538	8	7	theme	GSLs	1257:1260	arg1	forms					1248:1252	new and diverse lipid forms	1226:1252	new and diverse lipid forms of GSLs	1226:1260	It is expected that this method will help accelerate glycolipidomics analysis and disclose new and diverse lipid forms of GSLs.					
33534538	4	8	dep	contain	557:563	arg1	conserved					591:599	conserved	591:599	are conserved within a GSL species	587:620	In the first stage, carbohydrate fragments, which contain only glycans and thus are conserved within a GSL species, are directly matched to yield a species identification.					
33534538	8	9	theme	new	1226:1228	arg1	forms					1248:1252	new and diverse lipid forms	1226:1252	new and diverse lipid forms of GSLs	1226:1260	It is expected that this method will help accelerate glycolipidomics analysis and disclose new and diverse lipid forms of GSLs.					
33534538	2	10	from	determination	212:224	arg1	tissues					268:274	human tissues	262:274	human tissues	262:274	Thus, determination of the complete GSL compositions in human tissues is essential for comparative and functional studies of GSLs.					
33534538	1	11	theme	various	162:168	arg1	events					198:203	various biological and pathological events	162:203	various biological and pathological events	162:203	Glycosphingolipids (GSLs) play a key role in various biological and pathological events.					
33534538	0	12	theme	Lipid	57:61	arg1	Characterization					0:15	Characterization	0:15	Characterization of Glycosphingolipids and Their Diverse Lipid	0:61	Characterization of Glycosphingolipids and Their Diverse Lipid Forms through Two-Stage Matching of LC-MS/MS Spectra.					
33534538	7	13	theme	GSL	1069:1071	arg1	species					1073:1079	GSL species	1069:1079	GSL species	1069:1079	Furthermore, simple databases containing only a single lipid form per GSL species can be utilized to identify multiple GSL lipid forms.					
33534538	1	14	theme	biological	170:179	arg1	events					198:203	various biological and pathological events	162:203	various biological and pathological events	162:203	Glycosphingolipids (GSLs) play a key role in various biological and pathological events.					
33534538	7	15	theme	lipid	1054:1058	arg1	form					1060:1063	only a single lipid form	1040:1063	only a single lipid form	1040:1063	Furthermore, simple databases containing only a single lipid form per GSL species can be utilized to identify multiple GSL lipid forms.					
33534538	4	16	contain	contain	557:563	arg2	glycans					570:576	only glycans	565:576	only glycans	565:576	In the first stage, carbohydrate fragments, which contain only glycans and thus are conserved within a GSL species, are directly matched to yield a species identification.					
33534538	4	16	contain	contain	557:563	arg1	fragments					540:548	carbohydrate fragments	527:548	carbohydrate fragments	527:548	In the first stage, carbohydrate fragments, which contain only glycans and thus are conserved within a GSL species, are directly matched to yield a species identification.					
33534538	3	17	theme	glycolipidomics	409:423	arg1	analysis					425:432	glycolipidomics analysis	409:432	glycolipidomics analysis	409:432	In this work, a new strategy was developed for GSL characterization and glycolipidomics analysis based on two-stage matching of experimental and reference MS/MS spectra.					
33534538	5	18	dep	contain	757:763	arg1	shift					801:805	shift	801:805	shift due to lipid structural changes	801:837	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	2	19	theme	functional	309:318	arg1	studies					320:326	comparative and functional studies	293:326	comparative and functional studies of GSLs	293:334	Thus, determination of the complete GSL compositions in human tissues is essential for comparative and functional studies of GSLs.					
33534538	2	20	from	compositions	246:257	arg1	tissues					268:274	human tissues	262:274	human tissues	262:274	Thus, determination of the complete GSL compositions in human tissues is essential for comparative and functional studies of GSLs.					
33534538	1	21	theme	pathological	185:196	arg1	events					198:203	various biological and pathological events	162:203	various biological and pathological events	162:203	Glycosphingolipids (GSLs) play a key role in various biological and pathological events.					
33534538	3	22	theme	new	353:355	arg1	strategy					357:364	a new strategy	351:364	a new strategy	351:364	In this work, a new strategy was developed for GSL characterization and glycolipidomics analysis based on two-stage matching of experimental and reference MS/MS spectra.					
33534538	6	23	theme	new	936:938	arg1	strategy					940:947	This new strategy	931:947	This new strategy	931:947	This new strategy uses the whole spectrum for GSL characterization.					
33534538	5	24	theme	lipid	911:915	arg1	compositions					917:928	the lipid compositions	907:928	the lipid compositions	907:928	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	7	25	contain	containing	1029:1038	arg1	databases					1019:1027	simple databases	1012:1027	simple databases containing only a single lipid form per GSL species	1012:1079	Furthermore, simple databases containing only a single lipid form per GSL species can be utilized to identify multiple GSL lipid forms.					
33534538	7	25	contain	containing	1029:1038	arg2	form					1060:1063	only a single lipid form	1040:1063	only a single lipid form	1040:1063	Furthermore, simple databases containing only a single lipid form per GSL species can be utilized to identify multiple GSL lipid forms.					
33534538	0	26	theme	Glycosphingolipids	20:37	arg1	Characterization					0:15	Characterization	0:15	Characterization of Glycosphingolipids and Their Diverse Lipid	0:61	Characterization of Glycosphingolipids and Their Diverse Lipid Forms through Two-Stage Matching of LC-MS/MS Spectra.					
33534538	4	27	theme	only	565:568	arg1	glycans					570:576	only glycans	565:576	only glycans	565:576	In the first stage, carbohydrate fragments, which contain only glycans and thus are conserved within a GSL species, are directly matched to yield a species identification.					
33534538	5	28	theme	second	686:691	arg1	stage					693:697	the second stage	682:697	the second stage	682:697	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	2	29	theme	comparative	293:303	arg1	studies					320:326	comparative and functional studies	293:326	comparative and functional studies of GSLs	293:334	Thus, determination of the complete GSL compositions in human tissues is essential for comparative and functional studies of GSLs.					
33534538	3	30	theme	experimental	465:476	arg1	spectra					498:504	experimental and reference MS/MS spectra	465:504	spectra	498:504	In this work, a new strategy was developed for GSL characterization and glycolipidomics analysis based on two-stage matching of experimental and reference MS/MS spectra.					
33534538	0	31	theme	Diverse	49:55	arg1	Lipid					57:61	Their Diverse Lipid	43:61	Their Diverse Lipid	43:61	Characterization of Glycosphingolipids and Their Diverse Lipid Forms through Two-Stage Matching of LC-MS/MS Spectra.					
33534538	5	32	theme	rule-based	871:880	arg1	lipid					865:869	lipid rule-based	865:880	lipid rule-based matching	865:889	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	2	33	theme	complete	233:240	arg1	compositions					246:257	the complete GSL compositions	229:257	the complete GSL compositions in human tissues	229:274	Thus, determination of the complete GSL compositions in human tissues is essential for comparative and functional studies of GSLs.					
33534538	8	34	theme	lipid	1242:1246	arg1	forms					1248:1252	new and diverse lipid forms	1226:1252	new and diverse lipid forms of GSLs	1226:1260	It is expected that this method will help accelerate glycolipidomics analysis and disclose new and diverse lipid forms of GSLs.					
33534538	5	35	theme	lipid	814:818	arg1	changes					831:837	lipid structural changes	814:837	lipid structural changes	814:837	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	7	36	theme	GSL	1118:1120	arg1	forms					1128:1132	multiple GSL lipid forms	1109:1132	multiple GSL lipid forms	1109:1132	Furthermore, simple databases containing only a single lipid form per GSL species can be utilized to identify multiple GSL lipid forms.					
33534538	6	37	theme	GSL	977:979	arg1	characterization					981:996	GSL characterization	977:996	GSL characterization	977:996	This new strategy uses the whole spectrum for GSL characterization.					
33534538	7	38	theme	lipid	1122:1126	arg1	forms					1128:1132	multiple GSL lipid forms	1109:1132	multiple GSL lipid forms	1109:1132	Furthermore, simple databases containing only a single lipid form per GSL species can be utilized to identify multiple GSL lipid forms.					
33534538	7	39	theme	simple	1012:1017	arg1	databases					1019:1027	simple databases	1012:1027	simple databases containing only a single lipid form per GSL species	1012:1079	Furthermore, simple databases containing only a single lipid form per GSL species can be utilized to identify multiple GSL lipid forms.					
33534538	3	40	theme	MS/MS	492:496	arg1	spectra					498:504	experimental and reference MS/MS spectra	465:504	spectra	498:504	In this work, a new strategy was developed for GSL characterization and glycolipidomics analysis based on two-stage matching of experimental and reference MS/MS spectra.					
33534538	3	41	theme	GSL	384:386	arg1	characterization					388:403	GSL characterization	384:403	GSL characterization	384:403	In this work, a new strategy was developed for GSL characterization and glycolipidomics analysis based on two-stage matching of experimental and reference MS/MS spectra.					
33534538	5	42	from	species	742:748	arg1	fragments					711:719	glycolipid fragments	700:719	glycolipid fragments	700:719	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	3	43	theme	spectra	498:504	arg1	two-stage					443:451	two-stage	443:451	two-stage	443:451	In this work, a new strategy was developed for GSL characterization and glycolipidomics analysis based on two-stage matching of experimental and reference MS/MS spectra.					
33534538	4	44	theme	carbohydrate	527:538	arg1	fragments					540:548	carbohydrate fragments	527:548	carbohydrate fragments	527:548	In the first stage, carbohydrate fragments, which contain only glycans and thus are conserved within a GSL species, are directly matched to yield a species identification.					
33534538	2	45	theme	human	262:266	arg1	tissues					268:274	human tissues	262:274	human tissues	262:274	Thus, determination of the complete GSL compositions in human tissues is essential for comparative and functional studies of GSLs.					
33534538	4	46	theme	species	655:661	arg1	identification					663:676	a species identification	653:676	a species identification	653:676	In the first stage, carbohydrate fragments, which contain only glycans and thus are conserved within a GSL species, are directly matched to yield a species identification.					
33534538	2	47	theme	GSLs	331:334	arg1	studies					320:326	comparative and functional studies	293:326	comparative and functional studies of GSLs	293:334	Thus, determination of the complete GSL compositions in human tissues is essential for comparative and functional studies of GSLs.					
33534538	2	48	from	tissues	268:274	arg1	determination					212:224	determination	212:224	determination of the complete GSL compositions in human tissues	212:274	Thus, determination of the complete GSL compositions in human tissues is essential for comparative and functional studies of GSLs.					
33534538	5	49	contain	contain	757:763	arg2	glycans					784:790	glycans	784:790	glycans	784:790	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	5	49	contain	contain	757:763	arg1	fragments					711:719	glycolipid fragments	700:719	glycolipid fragments	700:719	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	5	49	contain	contain	757:763	arg2	lipid					774:778	the lipid	770:778	the lipid	770:778	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	3	50	theme	reference	482:490	arg1	spectra					498:504	experimental and reference MS/MS spectra	465:504	spectra	498:504	In this work, a new strategy was developed for GSL characterization and glycolipidomics analysis based on two-stage matching of experimental and reference MS/MS spectra.					
33534538	5	51	theme	matched	730:736	arg1	species					742:748	the matched GSL species	726:748	the matched GSL species	726:748	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	8	52	theme	diverse	1234:1240	arg1	forms					1248:1252	new and diverse lipid forms	1226:1252	new and diverse lipid forms of GSLs	1226:1260	It is expected that this method will help accelerate glycolipidomics analysis and disclose new and diverse lipid forms of GSLs.					
33534538	2	53	theme	compositions	246:257	arg1	determination					212:224	determination	212:224	determination of the complete GSL compositions in human tissues	212:274	Thus, determination of the complete GSL compositions in human tissues is essential for comparative and functional studies of GSLs.					
33534538	5	54	theme	structural	820:829	arg1	changes					831:837	lipid structural changes	814:837	lipid structural changes	814:837	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	4	55	theme	GSL	610:612	arg1	species					614:620	a GSL species	608:620	a GSL species	608:620	In the first stage, carbohydrate fragments, which contain only glycans and thus are conserved within a GSL species, are directly matched to yield a species identification.					
33534538	5	56	theme	GSL	738:740	arg1	species					742:748	the matched GSL species	726:748	the matched GSL species	726:748	In the second stage, glycolipid fragments from the matched GSL species, which contain both the lipid and glycans and thus shift due to lipid structural changes, are treated according to lipid rule-based matching to characterize the lipid compositions.					
33534538	8	57	theme	glycolipidomics	1188:1202	arg1	analysis					1204:1211	glycolipidomics analysis	1188:1211	glycolipidomics analysis	1188:1211	It is expected that this method will help accelerate glycolipidomics analysis and disclose new and diverse lipid forms of GSLs.					
33534538	2	58	theme	GSL	242:244	arg1	compositions					246:257	the complete GSL compositions	229:257	the complete GSL compositions in human tissues	229:274	Thus, determination of the complete GSL compositions in human tissues is essential for comparative and functional studies of GSLs.					
33534538	1	59	theme	key	150:152	arg1	role					154:157	a key role	148:157	a key role	148:157	Glycosphingolipids (GSLs) play a key role in various biological and pathological events.					
34786539	1	0	gly	Glycosylation	91:103	arg1	glycoprotein					175:186	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	4	1	theme	Binding	522:528	arg1	affinities					530:539	Binding affinities	522:539	Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN	522:583	Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions.					
34786539	5	2	theme	viral	671:675	arg1	infection					688:696	viral productive infection	671:696	viral productive infection	671:696	Although none of the CLRs supported viral productive infection, viral trans&cis-infection mediated by the CLRs were substantially changed among the different virions.					
34786539	7	3	theme	authentic	1096:1104	arg1	infection					1112:1120	authentic viral infection	1096:1120	authentic viral infection	1096:1120	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	1	4	theme	spike	169:173	arg1	glycoprotein					175:186	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	7	5	theme	viral	1106:1110	arg1	infection					1112:1120	authentic viral infection	1096:1120	authentic viral infection	1096:1120	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	4	6	theme	different	601:609	arg1	virions					626:632	the different glycan pattern virions	597:632	the different glycan pattern virions	597:632	Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions.					
34786539	4	7	theme	C-type	544:549	arg1	CLRs					569:572	CLRs	569:572	CLRs	569:572	Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions.					
34786539	4	7	theme	C-type	544:549	arg1	receptors					558:566	C-type lectin receptors	544:566	C-type lectin receptors (CLRs) DC&L-SIGN	544:583	Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions.					
34786539	5	8	theme	productive	677:686	arg1	infection					688:696	viral productive infection	671:696	viral productive infection	671:696	Although none of the CLRs supported viral productive infection, viral trans&cis-infection mediated by the CLRs were substantially changed among the different virions.					
34786539	7	9	theme	respiratory	1017:1027	arg1	cells					1035:1039	respiratory tract cells	1017:1039	respiratory tract cells	1017:1039	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	1	10	theme	glycoprotein	175:186	arg1	Glycosylation					91:103	Glycosylation	91:103	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	91:186	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	7	11	theme	tract	1029:1033	arg1	cells					1035:1039	respiratory tract cells	1017:1039	respiratory tract cells	1017:1039	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	4	12	theme	pattern	618:624	arg1	virions					626:632	the different glycan pattern virions	597:632	the different glycan pattern virions	597:632	Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions.					
34786539	3	13	theme	SARS-CoV-2	386:395	arg1	virions					397:403	SARS-CoV-2 virions	386:403	SARS-CoV-2 virions	386:403	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	6	14	theme	trimer	940:945	arg1	trimer					940:945	the spike postfusion trimer	919:945	the spike postfusion trimer	919:945	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	6	14	theme	trimer	940:945	arg1	site					911:914	the N1098 glycan site	894:914	the N1098 glycan site of the spike postfusion trimer	894:945	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	7	15	theme	SARS-CoV-2	1185:1194	arg1	infection					1196:1204	SARS-CoV-2 infection	1185:1204	SARS-CoV-2 infection	1185:1204	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	6	16	theme	high-mannose	854:865	arg1	Man5GlcNAc2					878:888	Man5GlcNAc2	878:888	Man5GlcNAc2	878:888	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	6	16	theme	high-mannose	854:865	arg1	structure					867:875	a high-mannose structure	852:875	a high-mannose structure (Man5GlcNAc2)	852:889	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	4	17	theme	glycan	611:616	arg1	virions					626:632	the different glycan pattern virions	597:632	the different glycan pattern virions	597:632	Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions.					
34786539	6	18	theme	postfusion	929:938	arg1	trimer					940:945	the spike postfusion trimer	919:945	the spike postfusion trimer	919:945	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	3	19	theme	origins	456:462	arg1	viruses					435:441	viruses	435:441	viruses of different origins with diverse spike protein glycan patterns	435:505	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	5	20	theme	different	783:791	arg1	virions					793:799	the different virions	779:799	the different virions	779:799	Although none of the CLRs supported viral productive infection, viral trans&cis-infection mediated by the CLRs were substantially changed among the different virions.					
34786539	6	21	theme	spike	923:927	arg1	trimer					940:945	the spike postfusion trimer	919:945	the spike postfusion trimer	919:945	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	7	22	theme	DC&L-SIGN	1172:1180	arg1	role					1164:1167	the attachment co-receptor role	1137:1167	the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention	1137:1219	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	3	23	theme	host	418:421	arg1	lines					428:432	various host cell lines	410:432	various host cell lines	410:432	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	1	24	theme	viral	197:201	arg1	entry					203:207	viral entry	197:207	viral entry	197:207	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	3	25	theme	various	410:416	arg1	lines					428:432	various host cell lines	410:432	various host cell lines	410:432	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	5	26	theme	CLRs	656:659	arg1	none					644:647	none	644:647	none of the CLRs	644:659	Although none of the CLRs supported viral productive infection, viral trans&cis-infection mediated by the CLRs were substantially changed among the different virions.					
34786539	6	27	theme	virions	839:845	arg1	trans&cis-infection					816:834	trans&cis-infection	816:834	trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer	816:945	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	0	28	theme	Epigenetic	0:9	arg1	glycosylation					11:23	Epigenetic glycosylation	0:23	Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors.	0:89	Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors.					
34786539	1	29	theme	severe	108:113	arg1	coronavirus					142:152	severe acute respiratory syndrome coronavirus 2	108:154	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	1	29	theme	severe	108:113	arg1	SARS-CoV-2					157:166	SARS-CoV-2	157:166	SARS-CoV-2	157:166	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	2	30	dep	genetic	269:275	arg1	code					277:280	code	277:280	code	277:280	While glycan site is determined by viral genetic code, glycosylation is completely dependent on host cell post-translational modification.					
34786539	0	31	theme	SARS-CoV-2	28:37	arg1	infection					52:60	SARS-CoV-2 impact viral infection	28:60	SARS-CoV-2 impact viral infection through DC&L-SIGN receptors	28:88	Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors.					
34786539	7	32	from	role	1164:1167	arg1	prevention					1210:1219	prevention	1210:1219	prevention	1210:1219	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	7	32	from	role	1164:1167	arg1	infection					1196:1204	SARS-CoV-2 infection	1185:1204	SARS-CoV-2 infection	1185:1204	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	7	33	theme	L-SIGN	983:988	arg1	co-expression					990:1002	L-SIGN co-expression	983:1002	L-SIGN co-expression with ACE2 on respiratory tract cells	983:1039	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	1	34	theme	acute	115:119	arg1	coronavirus					142:152	severe acute respiratory syndrome coronavirus 2	108:154	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	1	34	theme	acute	115:119	arg1	SARS-CoV-2					157:166	SARS-CoV-2	157:166	SARS-CoV-2	157:166	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	1	35	theme	immune	213:218	arg1	escape					220:225	immune escape	213:225	immune escape	213:225	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	2	36	theme	host	324:327	arg1	cell					329:332	host cell post-translational modification	324:364	host cell post-translational modification	324:364	While glycan site is determined by viral genetic code, glycosylation is completely dependent on host cell post-translational modification.					
34786539	7	37	with	co-expression	990:1002	arg1	ACE2					1009:1012	ACE2	1009:1012	ACE2	1009:1012	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	7	38	theme	attachment	1141:1150	arg1	role					1164:1167	the attachment co-receptor role	1137:1167	the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention	1137:1219	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	1	39	theme	respiratory	121:131	arg1	coronavirus					142:152	severe acute respiratory syndrome coronavirus 2	108:154	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	1	39	theme	respiratory	121:131	arg1	SARS-CoV-2					157:166	SARS-CoV-2	157:166	SARS-CoV-2	157:166	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	3	40	theme	glycan	491:496	arg1	patterns					498:505	diverse spike protein glycan patterns	469:505	diverse spike protein glycan patterns	469:505	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	0	41	theme	viral	46:50	arg1	infection					52:60	SARS-CoV-2 impact viral infection	28:60	SARS-CoV-2 impact viral infection through DC&L-SIGN receptors	28:88	Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors.					
34786539	3	42	theme	different	446:454	arg1	origins					456:462	different origins	446:462	different origins	446:462	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	3	43	theme	diverse	469:475	arg1	patterns					498:505	diverse spike protein glycan patterns	469:505	diverse spike protein glycan patterns	469:505	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	0	44	theme	impact	39:44	arg1	infection					52:60	SARS-CoV-2 impact viral infection	28:60	SARS-CoV-2 impact viral infection through DC&L-SIGN receptors	28:88	Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors.					
34786539	4	45	theme	receptors	558:566	arg1	DC&L-SIGN					575:583	C-type lectin receptors (CLRs) DC&L-SIGN	544:583	C-type lectin receptors (CLRs) DC&L-SIGN	544:583	Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions.					
34786539	7	46	gly	glycosylation	1079:1091	arg1	infection					1112:1120	authentic viral infection	1096:1120	authentic viral infection	1096:1120	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	6	47	theme	glycan	904:909	arg1	trimer					940:945	the spike postfusion trimer	919:945	the spike postfusion trimer	919:945	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	6	47	theme	glycan	904:909	arg1	site					911:914	the N1098 glycan site	894:914	the N1098 glycan site of the spike postfusion trimer	894:945	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	0	48	gly	glycosylation	11:23	arg1	infection					52:60	SARS-CoV-2 impact viral infection	28:60	SARS-CoV-2 impact viral infection through DC&L-SIGN receptors	28:88	Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors.					
34786539	7	49	theme	epigenetic	1068:1077	arg1	glycosylation					1079:1091	viral epigenetic glycosylation	1062:1091	viral epigenetic glycosylation in authentic viral infection	1062:1120	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	3	50	theme	spike	477:481	arg1	patterns					498:505	diverse spike protein glycan patterns	469:505	diverse spike protein glycan patterns	469:505	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	3	51	theme	cell	423:426	arg1	lines					428:432	various host cell lines	410:432	various host cell lines	410:432	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	4	52	theme	lectin	551:556	arg1	CLRs					569:572	CLRs	569:572	CLRs	569:572	Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions.					
34786539	4	52	theme	lectin	551:556	arg1	receptors					558:566	C-type lectin receptors	544:566	C-type lectin receptors (CLRs) DC&L-SIGN	544:583	Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions.					
34786539	5	53	theme	viral	699:703	arg1	trans&cis-infection					705:723	viral trans&cis-infection	699:723	viral trans&cis-infection mediated by the CLRs	699:744	Although none of the CLRs supported viral productive infection, viral trans&cis-infection mediated by the CLRs were substantially changed among the different virions.					
34786539	6	54	theme	N1098	898:902	arg1	trimer					940:945	the spike postfusion trimer	919:945	the spike postfusion trimer	919:945	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	6	54	theme	N1098	898:902	arg1	site					911:914	the N1098 glycan site	894:914	the N1098 glycan site of the spike postfusion trimer	894:945	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	1	55	theme	syndrome	133:140	arg1	coronavirus					142:152	severe acute respiratory syndrome coronavirus 2	108:154	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	1	55	theme	syndrome	133:140	arg1	SARS-CoV-2					157:166	SARS-CoV-2	157:166	SARS-CoV-2	157:166	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	0	56	theme	infection	52:60	arg1	glycosylation					11:23	Epigenetic glycosylation	0:23	Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors.	0:89	Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors.					
34786539	7	57	theme	co-receptor	1152:1162	arg1	role					1164:1167	the attachment co-receptor role	1137:1167	the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention	1137:1219	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	2	58	theme	glycan	234:239	arg1	site					241:244	glycan site	234:244	glycan site	234:244	While glycan site is determined by viral genetic code, glycosylation is completely dependent on host cell post-translational modification.					
34786539	1	59	theme	coronavirus	142:152	arg1	glycoprotein					175:186	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	1	60	gly	glycoprotein	175:186	arg1	glycoprotein					175:186	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein	108:186	Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape.					
34786539	7	61	theme	viral	1062:1066	arg1	glycosylation					1079:1091	viral epigenetic glycosylation	1062:1091	viral epigenetic glycosylation in authentic viral infection	1062:1120	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	2	62	dep	cell	329:332	arg1	modification					353:364	post-translational modification	334:364	host cell post-translational modification	324:364	While glycan site is determined by viral genetic code, glycosylation is completely dependent on host cell post-translational modification.					
34786539	3	63	with	viruses	435:441	arg1	patterns					498:505	diverse spike protein glycan patterns	469:505	diverse spike protein glycan patterns	469:505	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	2	64	theme	post-translational	334:351	arg1	modification					353:364	post-translational modification	334:364	host cell post-translational modification	324:364	While glycan site is determined by viral genetic code, glycosylation is completely dependent on host cell post-translational modification.					
34786539	7	65	from	co-expression	990:1002	arg1	cells					1035:1039	respiratory tract cells	1017:1039	respiratory tract cells	1017:1039	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	6	66	with	virions	839:845	arg1	Man5GlcNAc2					878:888	Man5GlcNAc2	878:888	Man5GlcNAc2	878:888	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	6	66	with	virions	839:845	arg1	structure					867:875	a high-mannose structure	852:875	a high-mannose structure (Man5GlcNAc2)	852:889	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
34786539	7	67	from	glycosylation	1079:1091	arg1	infection					1112:1120	authentic viral infection	1096:1120	authentic viral infection	1096:1120	Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.					
34786539	3	68	theme	protein	483:489	arg1	patterns					498:505	diverse spike protein glycan patterns	469:505	diverse spike protein glycan patterns	469:505	Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed.					
34786539	0	69	theme	DC&L-SIGN	70:78	arg1	receptors					80:88	DC&L-SIGN receptors	70:88	DC&L-SIGN receptors	70:88	Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors.					
34786539	6	70	from	site	911:914	arg1	trans&cis-infection					816:834	trans&cis-infection	816:834	trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer	816:945	Specifically, trans&cis-infection of virions with a high-mannose structure (Man5GlcNAc2) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced.					
32799357	7	0	theme	walls	2009:2013	arg1	features					1997:2004	the structural features	1982:2004	the structural features of walls	1982:2013	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	1	1	theme	Alexa	418:422	arg1	oligosaccharides					443:458	Alexa Fluor 488-labelled oligosaccharides	418:458	Alexa Fluor 488-labelled oligosaccharides	418:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	2	theme	xyloglucosyl	268:279	arg1	transferases					281:292	xyloglucan xyloglucosyl transferases	257:292	xyloglucan xyloglucosyl transferases	257:292	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	4	3	theme	majus	1015:1019	arg1	L.					1021:1022	Tropaeolum majus L.	1004:1022	Tropaeolum majus L.	1004:1022	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	4	3	theme	majus	1015:1019	arg1	nasturtium					992:1001	nasturtium	992:1001	nasturtium (Tropaeolum majus L.)	992:1023	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	1	4	theme	488-labelled	430:441	arg1	oligosaccharides					443:458	Alexa Fluor 488-labelled oligosaccharides	418:458	Alexa Fluor 488-labelled oligosaccharides	418:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	4	5	with	reaction	1124:1131	arg1	pair					1178:1181	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1138:1181	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1138:1181	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	2	6	theme	[α	661:662	arg1	acceptor					676:683	the penta-galacturonide [α(1-4)GalAp]5 acceptor	637:683	the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment	637:724	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	0	7	theme	Barley	47:52	arg1	transferases					78:89	Barley xyloglucan xyloglucosyl transferases	47:89	Barley xyloglucan xyloglucosyl transferases	47:89	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	5	8	theme	gene	1383:1386	arg1	family					1388:1393	the gene family	1379:1393	the gene family	1379:1393	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	1	9	theme	homo-	185:189	arg1	activities					221:230	the homo- and hetero-transglycosylation activities	181:230	the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides	181:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	7	10	theme	re-modelling	1957:1968	arg1	patterns					1921:1928	spatial patterns	1913:1928	spatial patterns of cell wall formation and re-modelling	1913:1968	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	1	11	theme	hetero-transglycosylation	195:219	arg1	activities					221:230	the homo- and hetero-transglycosylation activities	181:230	the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides	181:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	2	12	theme	defined	555:561	arg1	donor					563:567	the chemically defined donor	540:567	the chemically defined donor	540:567	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	1	13	theme	activities	379:388	arg1	XET					295:297	XET	295:297	XET	295:297	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	13	theme	activities	379:388	arg1	HvXET4					250:255	HvXET4	250:255	HvXET4	250:255	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	13	theme	activities	379:388	arg1	HvXET3					239:244	HvXET3	239:244	HvXET3	239:244	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	13	theme	activities	379:388	arg1	visualisation					356:368	the visualisation	352:368	the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides	352:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	14	from	activities	221:230	arg1	L.					343:344	Hordeum vulgare L.	327:344	Hordeum vulgare L.	327:344	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	14	from	activities	221:230	arg1	barley					319:324	barley	319:324	barley (Hordeum vulgare L.)	319:345	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	7	15	theme	cell	1933:1936	arg1	wall					1938:1941	cell wall formation and re-modelling	1933:1968	wall	1938:1941	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	1	16	dep	HvXET3	239:244	arg1	the					235:237	the	235:237	the	235:237	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	16	dep	HvXET3	239:244	arg1	transferases					281:292	xyloglucan xyloglucosyl transferases	257:292	xyloglucan xyloglucosyl transferases	257:292	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	4	17	theme	xyloglucan/[α	1142:1154	arg1	pair					1178:1181	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1138:1181	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1138:1181	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	3	18	theme	3D	757:758	arg1	models					770:775	3D molecular models	757:775	3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites	757:907	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	1	19	from	HvXET4	250:255	arg1	roots					406:410	young barley roots	393:410	young barley roots using Alexa Fluor 488-labelled oligosaccharides	393:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	20	theme	young	393:397	arg1	roots					406:410	young barley roots	393:410	young barley roots using Alexa Fluor 488-labelled oligosaccharides	393:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	5	21	theme	Expression	1231:1240	arg1	data					1242:1245	Expression data	1231:1245	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family	1231:1393	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	3	22	theme	HvXET4	791:796	arg1	models					770:775	3D molecular models	757:775	3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites	757:907	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	1	23	from	HvXET3	239:244	arg1	roots					406:410	young barley roots	393:410	young barley roots using Alexa Fluor 488-labelled oligosaccharides	393:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	3	24	with	HvXET3	780:785	arg1	substrates					853:862	the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates	803:862	the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates	803:862	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	7	25	theme	hetero-transglycosylation	1694:1718	arg1	activity					1720:1727	The discovered hetero-transglycosylation activity	1679:1727	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair	1679:1798	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	4	26	theme	Comparative	910:920	arg1	analyses					931:938	Comparative sequence analyses	910:938	Comparative sequence analyses of barley isoforms	910:957	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	4	27	theme	hetero-transglycosylation	1098:1122	arg1	reaction					1124:1131	the hetero-transglycosylation reaction	1094:1131	the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair	1094:1181	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	3	28	theme	HvXET3	780:785	arg1	models					770:775	3D molecular models	757:775	3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites	757:907	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	1	29	theme	Hordeum	327:333	arg1	L.					343:344	Hordeum vulgare L.	327:344	Hordeum vulgare L.	327:344	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	29	theme	Hordeum	327:333	arg1	barley					319:324	barley	319:324	barley (Hordeum vulgare L.)	319:345	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	3	30	dep	+5	877:878	arg1	to					874:875	to	874:875	to	874:875	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	0	31	from	block	17:21	arg1	wall					41:44	the plant cell wall	26:44	the plant cell wall	26:44	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	3	32	theme	XXXG	814:817	arg1	donor					819:823	docked XXXG donor	807:823	docked XXXG donor	807:823	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	0	33	theme	xyloglucan	54:63	arg1	transferases					78:89	Barley xyloglucan xyloglucosyl transferases	47:89	Barley xyloglucan xyloglucosyl transferases	47:89	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	6	34	theme	members	1618:1624	arg1	history					1573:1579	the evolutionary history	1556:1579	the evolutionary history	1556:1579	Conversely, HvXET3 and HvXET4 shared this relationship, when we examined the evolutionary history of 419 glycoside hydrolase 16 family members, spanning monocots, eudicots and a basal Angiosperm.					
32799357	3	35	from	models	770:775	arg1	subsites					880:887	the -4 to +5 subsites	867:887	the -4 to +5 subsites in the active sites	867:907	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	2	36	dep	acceptor	676:683	arg1	fragment					717:724	the homogalacturonan (pectin) fragment	687:724	the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment	637:724	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	7	37	theme	spatial	1913:1919	arg1	patterns					1921:1928	spatial patterns	1913:1928	spatial patterns of cell wall formation and re-modelling	1913:1968	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	7	38	with	activity	1720:1727	arg1	pair					1795:1798	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1755:1798	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1755:1798	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	2	39	with	reactions	525:533	arg1	substrates					582:591	the chemically defined donor and acceptor substrates	540:591	the chemically defined donor and acceptor substrates	540:591	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	3	40	theme	acceptor	844:851	arg1	substrates					853:862	the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates	803:862	the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates	803:862	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	4	41	theme	GalAp	1160:1164	arg1	pair					1178:1181	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1138:1181	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1138:1181	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	5	42	theme	HvXET	1311:1315	arg1	transcripts					1317:1327	HvXET transcripts	1311:1327	HvXET transcripts	1311:1327	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	1	43	from	visualisation	356:368	arg1	roots					406:410	young barley roots	393:410	young barley roots using Alexa Fluor 488-labelled oligosaccharides	393:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	4	44	from	nasturtium	992:1001	arg1	TmXET6.3					978:985	seed-localised TmXET6.3	963:985	seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.)	963:1023	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	4	44	from	nasturtium	992:1001	arg1	analyses					931:938	Comparative sequence analyses	910:938	Comparative sequence analyses of barley isoforms	910:957	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	5	45	theme	real-time	1259:1267	arg1	reaction					1299:1306	real-time quantitative polymerase chain reaction	1259:1306	real-time quantitative polymerase chain reaction of HvXET transcripts	1259:1327	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	4	46	theme	barley	943:948	arg1	isoforms					950:957	barley isoforms	943:957	barley isoforms	943:957	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	5	47	theme	polymerase	1282:1291	arg1	reaction					1299:1306	real-time quantitative polymerase chain reaction	1259:1306	real-time quantitative polymerase chain reaction of HvXET transcripts	1259:1327	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	7	48	theme	xyloglucan-pectin	1848:1864	arg1	heteropolymers					1866:1879	xyloglucan-pectin heteropolymers	1848:1879	xyloglucan-pectin heteropolymers	1848:1879	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	3	49	theme	active	896:901	arg1	sites					903:907	the active sites	892:907	the active sites	892:907	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	0	50	theme	cell	36:39	arg1	wall					41:44	the plant cell wall	26:44	the plant cell wall	26:44	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	7	51	theme	roles	1839:1843	arg1	background					1825:1834	the background	1821:1834	the background of roles of xyloglucan-pectin heteropolymers	1821:1879	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	7	52	dep	wall	1938:1941	arg1	formation					1943:1951	formation	1943:1951	formation	1943:1951	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	5	53	theme	profiles	1367:1374	arg1	reaction					1299:1306	real-time quantitative polymerase chain reaction	1259:1306	real-time quantitative polymerase chain reaction of HvXET transcripts	1259:1327	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	5	53	theme	profiles	1367:1374	arg1	heatmap					1345:1351	a clustered heatmap	1333:1351	a clustered heatmap of expression profiles of the gene family	1333:1393	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	3	54	with	HvXET4	791:796	arg1	substrates					853:862	the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates	803:862	the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates	803:862	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	0	55	dep	block	17:21	arg1	link					91:94	link	91:94	link covalently xyloglucan and anionic oligosaccharides derived from pectin	91:165	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	7	56	theme	GalAp	1777:1781	arg1	pair					1795:1798	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1755:1798	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1755:1798	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	4	57	theme	Tropaeolum	1004:1013	arg1	L.					1021:1022	Tropaeolum majus L.	1004:1022	Tropaeolum majus L.	1004:1022	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	4	57	theme	Tropaeolum	1004:1013	arg1	nasturtium					992:1001	nasturtium	992:1001	nasturtium (Tropaeolum majus L.)	992:1023	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	1	58	from	barley	319:324	arg1	XET					295:297	XET	295:297	XET	295:297	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	58	from	barley	319:324	arg1	HvXET4					250:255	HvXET4	250:255	HvXET4	250:255	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	58	from	barley	319:324	arg1	activities					221:230	the homo- and hetero-transglycosylation activities	181:230	the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides	181:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	58	from	barley	319:324	arg1	HvXET3					239:244	HvXET3	239:244	HvXET3	239:244	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	58	from	barley	319:324	arg1	visualisation					356:368	the visualisation	352:368	the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides	352:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	5	59	theme	clustered	1335:1343	arg1	heatmap					1345:1351	a clustered heatmap	1333:1351	a clustered heatmap of expression profiles of the gene family	1333:1393	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	1	60	theme	xyloglucan	257:266	arg1	transferases					281:292	xyloglucan xyloglucosyl transferases	257:292	xyloglucan xyloglucosyl transferases	257:292	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	2	61	theme	xyloglucan	616:625	arg1	donor					627:631	the xyloglucan donor	612:631	the xyloglucan donor	612:631	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	1	62	theme	Fluor	424:428	arg1	oligosaccharides					443:458	Alexa Fluor 488-labelled oligosaccharides	418:458	Alexa Fluor 488-labelled oligosaccharides	418:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	7	63	theme	xyloglucan/[α	1759:1771	arg1	pair					1795:1798	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1755:1798	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1755:1798	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	7	64	theme	structural	1986:1995	arg1	features					1997:2004	the structural features	1982:2004	the structural features of walls	1982:2013	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	2	65	theme	penta-galacturonide	641:659	arg1	acceptor					676:683	the penta-galacturonide [α(1-4)GalAp]5 acceptor	637:683	the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment	637:724	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	7	66	dep	discussed	1803:1811	arg1	participate					1898:1908	participate	1898:1908	may participate in spatial patterns of cell wall formation and re-modelling	1894:1968	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	7	66	dep	discussed	1803:1811	arg1	affect					1975:1980	affect	1975:1980	affect the structural features of walls	1975:2013	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	7	67	from	activity	1720:1727	arg1	HvXET4					1743:1748	HvXET4	1743:1748	HvXET4	1743:1748	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	7	67	from	activity	1720:1727	arg1	HvXET3					1732:1737	HvXET3	1732:1737	HvXET3	1732:1737	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	4	68	theme	mutants	1054:1060	arg1	engineering					1039:1049	the engineering	1035:1049	the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity	1035:1228	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	0	69	theme	anionic	122:128	arg1	oligosaccharides					130:145	xyloglucan and anionic oligosaccharides	107:145	oligosaccharides	130:145	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	5	70	theme	family	1388:1393	arg1	profiles					1367:1374	expression profiles	1356:1374	expression profiles of the gene family	1356:1393	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	2	71	theme	donor	563:567	arg1	substrates					582:591	the chemically defined donor and acceptor substrates	540:591	the chemically defined donor and acceptor substrates	540:591	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	6	72	theme	basal	1661:1665	arg1	Angiosperm					1667:1676	a basal Angiosperm	1659:1676	a basal Angiosperm	1659:1676	Conversely, HvXET3 and HvXET4 shared this relationship, when we examined the evolutionary history of 419 glycoside hydrolase 16 family members, spanning monocots, eudicots and a basal Angiosperm.					
32799357	4	73	theme	TmXET6.3	1065:1072	arg1	mutants					1054:1060	mutants	1054:1060	mutants of TmXET6.3	1054:1072	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	2	74	theme	pectin	709:714	arg1	fragment					717:724	the homogalacturonan (pectin) fragment	687:724	the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment	637:724	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	7	75	theme	wall	1938:1941	arg1	patterns					1921:1928	spatial patterns	1913:1928	spatial patterns of cell wall formation and re-modelling	1913:1968	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	3	76	from	subsites	880:887	arg1	HvXET4					791:796	HvXET4	791:796	HvXET4	791:796	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	3	76	from	subsites	880:887	arg1	HvXET3					780:785	HvXET3	780:785	HvXET3	780:785	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	3	76	from	subsites	880:887	arg1	sites					903:907	the active sites	892:907	the active sites	892:907	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	3	76	from	subsites	880:887	arg1	models					770:775	3D molecular models	757:775	3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites	757:907	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	2	77	theme	homogalacturonan	691:706	arg1	fragment					717:724	the homogalacturonan (pectin) fragment	687:724	the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment	637:724	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	3	78	theme	molecular	760:768	arg1	models					770:775	3D molecular models	757:775	3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites	757:907	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	1	79	theme	barley	399:404	arg1	roots					406:410	young barley roots	393:410	young barley roots using Alexa Fluor 488-labelled oligosaccharides	393:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	2	80	theme	acceptor	573:580	arg1	substrates					582:591	the chemically defined donor and acceptor substrates	540:591	the chemically defined donor and acceptor substrates	540:591	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	1	81	from	roots	406:410	arg1	XET					295:297	XET	295:297	XET	295:297	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	81	from	roots	406:410	arg1	HvXET4					250:255	HvXET4	250:255	HvXET4	250:255	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	81	from	roots	406:410	arg1	HvXET3					239:244	HvXET3	239:244	HvXET3	239:244	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	81	from	roots	406:410	arg1	visualisation					356:368	the visualisation	352:368	the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides	352:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	3	82	theme	[α	829:830	arg1	acceptor					844:851	[α(1-4)GalAp]5 acceptor	829:851	[α(1-4)GalAp]5 acceptor	829:851	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	0	83	theme	xyloglucosyl	65:76	arg1	transferases					78:89	Barley xyloglucan xyloglucosyl transferases	47:89	Barley xyloglucan xyloglucosyl transferases	47:89	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	1	84	theme	vulgare	335:341	arg1	L.					343:344	Hordeum vulgare L.	327:344	Hordeum vulgare L.	327:344	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	1	84	theme	vulgare	335:341	arg1	barley					319:324	barley	319:324	barley (Hordeum vulgare L.)	319:345	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	7	85	theme	discovered	1683:1692	arg1	activity					1720:1727	The discovered hetero-transglycosylation activity	1679:1727	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair	1679:1798	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	4	86	theme	sequence	922:929	arg1	analyses					931:938	Comparative sequence analyses	910:938	Comparative sequence analyses of barley isoforms	910:957	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	1	87	dep	XET	295:297	arg1	EC					300:301	EC 2.4.1.207	300:311	EC 2.4.1.207	300:311	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	2	88	theme	GalAp	668:672	arg1	acceptor					676:683	the penta-galacturonide [α(1-4)GalAp]5 acceptor	637:683	the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment	637:724	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	3	89	theme	donor	819:823	arg1	substrates					853:862	the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates	803:862	the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates	803:862	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	1	90	from	activities	379:388	arg1	roots					406:410	young barley roots	393:410	young barley roots using Alexa Fluor 488-labelled oligosaccharides	393:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	6	91	theme	family	1611:1616	arg1	members					1618:1624	419 glycoside hydrolase 16 family members	1584:1624	419 glycoside hydrolase 16 family members	1584:1624	Conversely, HvXET3 and HvXET4 shared this relationship, when we examined the evolutionary history of 419 glycoside hydrolase 16 family members, spanning monocots, eudicots and a basal Angiosperm.					
32799357	3	92	theme	GalAp	836:840	arg1	acceptor					844:851	[α(1-4)GalAp]5 acceptor	829:851	[α(1-4)GalAp]5 acceptor	829:851	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	5	93	theme	monophyletic	1462:1473	arg1	origin					1475:1480	the monophyletic origin	1458:1480	the monophyletic origin	1458:1480	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	4	94	theme	wild-type	1190:1198	arg1	TmXET6.3					1200:1207	wild-type TmXET6.3	1190:1207	wild-type TmXET6.3	1190:1207	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	5	95	theme	chain	1293:1297	arg1	reaction					1299:1306	real-time quantitative polymerase chain reaction	1259:1306	real-time quantitative polymerase chain reaction of HvXET transcripts	1259:1327	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	3	96	theme	docked	807:812	arg1	donor					819:823	docked XXXG donor	807:823	docked XXXG donor	807:823	This activity is supported by 3D molecular models of HvXET3 and HvXET4 with the docked XXXG donor and [α(1-4)GalAp]5 acceptor substrates at the -4 to +5 subsites in the active sites.					
32799357	0	97	theme	xyloglucan	107:116	arg1	oligosaccharides					130:145	xyloglucan and anionic oligosaccharides	107:145	oligosaccharides	130:145	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	6	98	theme	hydrolase	1598:1606	arg1	members					1618:1624	419 glycoside hydrolase 16 family members	1584:1624	419 glycoside hydrolase 16 family members	1584:1624	Conversely, HvXET3 and HvXET4 shared this relationship, when we examined the evolutionary history of 419 glycoside hydrolase 16 family members, spanning monocots, eudicots and a basal Angiosperm.					
32799357	2	99	theme	transglycosylation	506:523	arg1	reactions					525:533	the transglycosylation reactions	502:533	the transglycosylation reactions with the chemically defined donor and acceptor substrates	502:591	We discover that these isozymes catalyse the transglycosylation reactions with the chemically defined donor and acceptor substrates, specifically with the xyloglucan donor and the penta-galacturonide [α(1-4)GalAp]5 acceptor - the homogalacturonan (pectin) fragment.					
32799357	0	100	theme	building	8:15	arg1	block					17:21	Another building block	0:21	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.	0:166	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	1	101	theme	visualisation	356:368	arg1	activities					221:230	the homo- and hetero-transglycosylation activities	181:230	the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides	181:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	0	102	attach	derived	147:153	arg1	pectin					160:165	pectin	160:165	pectin	160:165	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	0	102	attach	derived	147:153	arg2	oligosaccharides					130:145	xyloglucan and anionic oligosaccharides	107:145	oligosaccharides	130:145	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	4	103	theme	seed-localised	963:976	arg1	TmXET6.3					978:985	seed-localised TmXET6.3	963:985	seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.)	963:1023	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	4	104	theme	substrate	1168:1176	arg1	pair					1178:1181	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1138:1181	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1138:1181	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	7	105	theme	heteropolymers	1866:1879	arg1	roles					1839:1843	roles	1839:1843	roles of xyloglucan-pectin heteropolymers	1839:1879	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	5	106	theme	quantitative	1269:1280	arg1	reaction					1299:1306	real-time quantitative polymerase chain reaction	1259:1306	real-time quantitative polymerase chain reaction of HvXET transcripts	1259:1327	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	0	107	theme	plant	30:34	arg1	wall					41:44	the plant cell wall	26:44	the plant cell wall	26:44	Another building block in the plant cell wall: Barley xyloglucan xyloglucosyl transferases link covalently xyloglucan and anionic oligosaccharides derived from pectin.					
32799357	4	108	theme	isoforms	950:957	arg1	TmXET6.3					978:985	seed-localised TmXET6.3	963:985	seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.)	963:1023	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	4	108	theme	isoforms	950:957	arg1	analyses					931:938	Comparative sequence analyses	910:938	Comparative sequence analyses of barley isoforms	910:957	Comparative sequence analyses of barley isoforms and seed-localised TmXET6.3 from nasturtium (Tropaeolum majus L.) permitted the engineering of mutants of TmXET6.3 that could catalyse the hetero-transglycosylation reaction with the xyloglucan/[α(1-4)GalAp]5 substrate pair, while wild-type TmXET6.3 lacked this activity.					
32799357	5	109	theme	expression	1356:1365	arg1	profiles					1367:1374	expression profiles	1356:1374	expression profiles of the gene family	1356:1393	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	6	110	theme	glycoside	1588:1596	arg1	members					1618:1624	419 glycoside hydrolase 16 family members	1584:1624	419 glycoside hydrolase 16 family members	1584:1624	Conversely, HvXET3 and HvXET4 shared this relationship, when we examined the evolutionary history of 419 glycoside hydrolase 16 family members, spanning monocots, eudicots and a basal Angiosperm.					
32799357	6	111	theme	evolutionary	1560:1571	arg1	history					1573:1579	the evolutionary history	1556:1579	the evolutionary history	1556:1579	Conversely, HvXET3 and HvXET4 shared this relationship, when we examined the evolutionary history of 419 glycoside hydrolase 16 family members, spanning monocots, eudicots and a basal Angiosperm.					
32799357	5	112	theme	transcripts	1317:1327	arg1	reaction					1299:1306	real-time quantitative polymerase chain reaction	1259:1306	real-time quantitative polymerase chain reaction of HvXET transcripts	1259:1327	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	5	112	theme	transcripts	1317:1327	arg1	heatmap					1345:1351	a clustered heatmap	1333:1351	a clustered heatmap of expression profiles of the gene family	1333:1393	Expression data obtained by real-time quantitative polymerase chain reaction of HvXET transcripts and a clustered heatmap of expression profiles of the gene family revealed that HvXET3 and HvXET6 co-expressed but did not share the monophyletic origin.					
32799357	1	113	theme	HvXET3	239:244	arg1	activities					221:230	the homo- and hetero-transglycosylation activities	181:230	the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides	181:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
32799357	7	114	theme	substrate	1785:1793	arg1	pair					1795:1798	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1755:1798	the xyloglucan/[α(1-4)GalAp]5 substrate pair	1755:1798	The discovered hetero-transglycosylation activity in HvXET3 and HvXET4 with the xyloglucan/[α(1-4)GalAp]5 substrate pair is discussed against the background of roles of xyloglucan-pectin heteropolymers and how they may participate in spatial patterns of cell wall formation and re-modelling, and affect the structural features of walls.					
32799357	1	115	theme	HvXET4	250:255	arg1	activities					221:230	the homo- and hetero-transglycosylation activities	181:230	the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides	181:458	We report on the homo- and hetero-transglycosylation activities of the HvXET3 and HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley (Hordeum vulgare L.), and the visualisation of these activities in young barley roots using Alexa Fluor 488-labelled oligosaccharides.					
34665908	0	0	theme	Glycoconjugate	91:104	arg1	Development					114:124	Glycoconjugate Vaccine Development	91:124	Glycoconjugate Vaccine Development	91:124	Total Syntheses of Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075 for Glycoconjugate Vaccine Development.					
34665908	4	1	theme	first	530:534	arg1	synthesis					542:550	the first total synthesis	526:550	the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues	526:657	Here, we report the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues.					
34665908	5	2	theme	linkages	688:695	arg1	construction					664:675	The construction	660:675	The construction of 1,2-cis linkages between the rare sugars	660:719	The construction of 1,2-cis linkages between the rare sugars relies on a double-serial inversion strategy.					
34665908	4	3	theme	AB5075	627:632	arg1	unit					606:609	the densely functionalized trisaccharide repeating unit	555:609	the densely functionalized trisaccharide repeating unit of A. baumannii AB5075	555:632	Here, we report the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues.					
34665908	1	4	theme	serious	192:198	arg1	infections					211:220	serious nosocomial infections	192:220	serious nosocomial infections	192:220	Acinetobacter baumannii is an opportunistic pathogen that causes serious nosocomial infections.					
34665908	0	5	theme	Vaccine	106:112	arg1	Development					114:124	Glycoconjugate Vaccine Development	91:124	Glycoconjugate Vaccine Development	91:124	Total Syntheses of Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075 for Glycoconjugate Vaccine Development.					
34665908	4	6	dep	A.	614:615	arg1	baumannii					617:625	baumannii	617:625	baumannii	617:625	Here, we report the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues.					
34665908	5	7	theme	rare	709:712	arg1	sugars					714:719	the rare sugars	705:719	the rare sugars	705:719	The construction of 1,2-cis linkages between the rare sugars relies on a double-serial inversion strategy.					
34665908	2	8	theme	wound	311:315	arg1	infections					317:326	wound infections	311:326	wound infections	311:326	One of the multidrug-resistant strains, AB5075, can result in bacteremia, pneumonia and wound infections associated with high morbidity and mortality.					
34665908	1	9	theme	nosocomial	200:209	arg1	infections					211:220	serious nosocomial infections	192:220	serious nosocomial infections	192:220	Acinetobacter baumannii is an opportunistic pathogen that causes serious nosocomial infections.					
34665908	3	10	theme	unique	391:396	arg1	glycans					398:404	The structurally unique glycans	374:404	The structurally unique glycans on the surface of these bacteria	374:437	The structurally unique glycans on the surface of these bacteria are attractive targets for the development of glycoconjugate vaccines.					
34665908	3	10	theme	unique	391:396	arg1	targets					454:460	attractive targets	443:460	attractive targets for the development of glycoconjugate vaccines	443:507	The structurally unique glycans on the surface of these bacteria are attractive targets for the development of glycoconjugate vaccines.					
34665908	3	11	theme	attractive	443:452	arg1	glycans					398:404	The structurally unique glycans	374:404	The structurally unique glycans on the surface of these bacteria	374:437	The structurally unique glycans on the surface of these bacteria are attractive targets for the development of glycoconjugate vaccines.					
34665908	3	11	theme	attractive	443:452	arg1	targets					454:460	attractive targets	443:460	attractive targets for the development of glycoconjugate vaccines	443:507	The structurally unique glycans on the surface of these bacteria are attractive targets for the development of glycoconjugate vaccines.					
34665908	6	12	theme	stereoselective	846:860	arg1	chain					945:949	the (S)-3-hydroxybutanoyl chain	919:949	the (S)-3-hydroxybutanoyl chain	919:949	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	6	12	theme	stereoselective	846:860	arg1	glycosylations					862:875	stereoselective glycosylations	846:875	stereoselective glycosylations	846:875	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	6	12	theme	stereoselective	846:860	arg1	installation					882:893	the installation	878:893	the installation of acetamido groups	878:913	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	6	13	theme	-3-hydroxybutanoyl	926:943	arg1	chain					945:949	the (S)-3-hydroxybutanoyl chain	919:949	the (S)-3-hydroxybutanoyl chain	919:949	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	6	13	theme	-3-hydroxybutanoyl	926:943	arg1	glycosylations					862:875	stereoselective glycosylations	846:875	stereoselective glycosylations	846:875	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	0	14	theme	Total	0:4	arg1	Syntheses					6:14	Total Syntheses	0:14	Total Syntheses of Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075 for Glycoconjugate Vaccine Development.	0:125	Total Syntheses of Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075 for Glycoconjugate Vaccine Development.					
34665908	4	15	theme	unit	606:609	arg1	analogues					649:657	two analogues	645:657	the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues	526:657	Here, we report the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues.					
34665908	4	15	theme	unit	606:609	arg1	synthesis					542:550	the first total synthesis	526:550	the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues	526:657	Here, we report the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues.					
34665908	5	16	theme	1,2-cis	680:686	arg1	linkages					688:695	1,2-cis linkages	680:695	1,2-cis linkages	680:695	The construction of 1,2-cis linkages between the rare sugars relies on a double-serial inversion strategy.					
34665908	2	17	theme	high	344:347	arg1	morbidity					349:357	morbidity	349:357	morbidity	349:357	One of the multidrug-resistant strains, AB5075, can result in bacteremia, pneumonia and wound infections associated with high morbidity and mortality.					
34665908	0	18	theme	Conjugation-Ready	19:35	arg1	Units					47:51	Conjugation-Ready Repeating Units	19:51	Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075	19:85	Total Syntheses of Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075 for Glycoconjugate Vaccine Development.					
34665908	4	19	theme	repeating	596:604	arg1	unit					606:609	the densely functionalized trisaccharide repeating unit	555:609	the densely functionalized trisaccharide repeating unit of A. baumannii AB5075	555:632	Here, we report the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues.					
34665908	5	20	theme	double-serial	733:745	arg1	strategy					757:764	a double-serial inversion strategy	731:764	a double-serial inversion strategy	731:764	The construction of 1,2-cis linkages between the rare sugars relies on a double-serial inversion strategy.					
34665908	6	21	theme	conditions	823:832	arg1	selection					781:789	The judicious selection	767:789	The judicious selection of building blocks and reaction conditions	767:832	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	4	22	theme	A.	614:615	arg1	AB5075					627:632	A. baumannii AB5075	614:632	A. baumannii AB5075	614:632	Here, we report the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues.					
34665908	5	23	theme	inversion	747:755	arg1	strategy					757:764	a double-serial inversion strategy	731:764	a double-serial inversion strategy	731:764	The construction of 1,2-cis linkages between the rare sugars relies on a double-serial inversion strategy.					
34665908	1	24	theme	Acinetobacter	127:139	arg1	baumannii					141:149	Acinetobacter baumannii	127:149	Acinetobacter baumannii	127:149	Acinetobacter baumannii is an opportunistic pathogen that causes serious nosocomial infections.					
34665908	1	24	theme	Acinetobacter	127:139	arg1	pathogen					171:178	an opportunistic pathogen	154:178	an opportunistic pathogen that causes serious nosocomial infections	154:220	Acinetobacter baumannii is an opportunistic pathogen that causes serious nosocomial infections.					
34665908	0	25	theme	Units	47:51	arg1	Syntheses					6:14	Total Syntheses	0:14	Total Syntheses of Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075 for Glycoconjugate Vaccine Development.	0:125	Total Syntheses of Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075 for Glycoconjugate Vaccine Development.					
34665908	6	26	theme	S	924:924	arg1	chain					945:949	the (S)-3-hydroxybutanoyl chain	919:949	the (S)-3-hydroxybutanoyl chain	919:949	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	6	26	theme	S	924:924	arg1	glycosylations					862:875	stereoselective glycosylations	846:875	stereoselective glycosylations	846:875	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	3	27	theme	glycoconjugate	485:498	arg1	vaccines					500:507	glycoconjugate vaccines	485:507	glycoconjugate vaccines	485:507	The structurally unique glycans on the surface of these bacteria are attractive targets for the development of glycoconjugate vaccines.					
34665908	0	28	theme	Repeating	37:45	arg1	Units					47:51	Conjugation-Ready Repeating Units	19:51	Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075	19:85	Total Syntheses of Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075 for Glycoconjugate Vaccine Development.					
34665908	3	29	from	glycans	398:404	arg1	surface					413:419	the surface	409:419	the surface of these bacteria	409:437	The structurally unique glycans on the surface of these bacteria are attractive targets for the development of glycoconjugate vaccines.					
34665908	6	30	theme	reaction	814:821	arg1	conditions					823:832	reaction conditions	814:832	reaction conditions	814:832	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	3	31	theme	vaccines	500:507	arg1	development					470:480	the development	466:480	the development of glycoconjugate vaccines	466:507	The structurally unique glycans on the surface of these bacteria are attractive targets for the development of glycoconjugate vaccines.					
34665908	3	32	theme	bacteria	430:437	arg1	surface					413:419	the surface	409:419	the surface of these bacteria	409:437	The structurally unique glycans on the surface of these bacteria are attractive targets for the development of glycoconjugate vaccines.					
34665908	0	33	theme	baumannii	70:78	arg1	Units					47:51	Conjugation-Ready Repeating Units	19:51	Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075	19:85	Total Syntheses of Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075 for Glycoconjugate Vaccine Development.					
34665908	4	34	theme	trisaccharide	582:594	arg1	unit					606:609	the densely functionalized trisaccharide repeating unit	555:609	the densely functionalized trisaccharide repeating unit of A. baumannii AB5075	555:632	Here, we report the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues.					
34665908	2	35	theme	strains	254:260	arg1	One					223:225	One	223:225	One	223:225	One of the multidrug-resistant strains, AB5075, can result in bacteremia, pneumonia and wound infections associated with high morbidity and mortality.					
34665908	2	35	theme	strains	254:260	arg1	AB5075					263:268	AB5075	263:268	AB5075	263:268	One of the multidrug-resistant strains, AB5075, can result in bacteremia, pneumonia and wound infections associated with high morbidity and mortality.					
34665908	2	35	theme	strains	254:260	arg1	strains					254:260	the multidrug-resistant strains	230:260	the multidrug-resistant strains	230:260	One of the multidrug-resistant strains, AB5075, can result in bacteremia, pneumonia and wound infections associated with high morbidity and mortality.					
34665908	6	36	theme	groups	908:913	arg1	chain					945:949	the (S)-3-hydroxybutanoyl chain	919:949	the (S)-3-hydroxybutanoyl chain	919:949	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	6	36	theme	groups	908:913	arg1	glycosylations					862:875	stereoselective glycosylations	846:875	stereoselective glycosylations	846:875	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	6	36	theme	groups	908:913	arg1	installation					882:893	the installation	878:893	the installation of acetamido groups	878:913	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	6	37	theme	blocks	803:808	arg1	selection					781:789	The judicious selection	767:789	The judicious selection of building blocks and reaction conditions	767:832	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	4	38	theme	functionalized	567:580	arg1	unit					606:609	the densely functionalized trisaccharide repeating unit	555:609	the densely functionalized trisaccharide repeating unit of A. baumannii AB5075	555:632	Here, we report the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues.					
34665908	2	39	theme	multidrug-resistant	234:252	arg1	strains					254:260	the multidrug-resistant strains	230:260	the multidrug-resistant strains	230:260	One of the multidrug-resistant strains, AB5075, can result in bacteremia, pneumonia and wound infections associated with high morbidity and mortality.					
34665908	6	40	theme	building	794:801	arg1	blocks					803:808	building blocks	794:808	building blocks	794:808	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	6	41	theme	acetamido	898:906	arg1	groups					908:913	acetamido groups	898:913	acetamido groups	898:913	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
34665908	1	42	theme	opportunistic	157:169	arg1	baumannii					141:149	Acinetobacter baumannii	127:149	Acinetobacter baumannii	127:149	Acinetobacter baumannii is an opportunistic pathogen that causes serious nosocomial infections.					
34665908	1	42	theme	opportunistic	157:169	arg1	pathogen					171:178	an opportunistic pathogen	154:178	an opportunistic pathogen that causes serious nosocomial infections	154:220	Acinetobacter baumannii is an opportunistic pathogen that causes serious nosocomial infections.					
34665908	0	43	theme	Acinetobacter	56:68	arg1	baumannii					70:78	Acinetobacter baumannii AB5075	56:85	Acinetobacter baumannii AB5075	56:85	Total Syntheses of Conjugation-Ready Repeating Units of Acinetobacter baumannii AB5075 for Glycoconjugate Vaccine Development.					
34665908	4	44	theme	total	536:540	arg1	synthesis					542:550	the first total synthesis	526:550	the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues	526:657	Here, we report the first total synthesis of the densely functionalized trisaccharide repeating unit of A. baumannii AB5075 as well as two analogues.					
34665908	6	45	theme	judicious	771:779	arg1	selection					781:789	The judicious selection	767:789	The judicious selection of building blocks and reaction conditions	767:832	The judicious selection of building blocks and reaction conditions allowed for stereoselective glycosylations, the installation of acetamido groups and the (S)-3-hydroxybutanoyl chain.					
32686492	5	0	dep	glucose	886:892	arg1	1→6					881:883	1→6	881:883	1→6	881:883	The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose.					
32686492	0	1	theme	Imperial	85:92	arg1	Chrysanthemum					94:106	Imperial Chrysanthemum	85:106	Imperial Chrysanthemum	85:106	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	0	2	from	characterization	11:26	arg1	Chrysanthemum					94:106	Imperial Chrysanthemum	85:106	Imperial Chrysanthemum	85:106	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	6	3	theme	bacteria	1020:1027	arg1	production					994:1003	the acid production	985:1003	the acid production of lactic acid bacteria	985:1027	Besides, ICP-1 can speed up the acid production of lactic acid bacteria and promote the growth and proliferation of lactic acid bacteria effectively.					
32686492	1	4	theme	Imperial	257:264	arg1	Chrysanthemum					266:278	Imperial Chrysanthemum	257:278	Imperial Chrysanthemum	257:278	A novel water-soluble polysaccharide, named ICP-1, was isolated and purified by Sephadex G-200 after extracting the crude polysaccharide (ICP) from Imperial Chrysanthemum.					
32686492	6	5	theme	acid	989:992	arg1	production					994:1003	the acid production	985:1003	the acid production of lactic acid bacteria	985:1027	Besides, ICP-1 can speed up the acid production of lactic acid bacteria and promote the growth and proliferation of lactic acid bacteria effectively.					
32686492	3	6	theme	prebiotic	636:644	arg1	potential					646:654	prebiotic potential	636:654	prebiotic potential	636:654	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	5	7	theme	1→3,6	941:945	arg1	mannose					948:954	(1→3,6) mannose	940:954	(1→3,6) mannose	940:954	The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose.					
32686492	3	8	theme	bacteria	522:529	arg1	proliferation					493:505	proliferation	493:505	proliferation	493:505	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	3	8	theme	bacteria	522:529	arg1	tests					549:553	the tolerance tests	535:553	the tolerance tests of simulated gastrointestinal fluid	535:589	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	3	8	theme	bacteria	522:529	arg1	production					478:487	acid production	473:487	acid production	473:487	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	2	9	theme	ICP-1	316:320	arg1	characterization					296:311	The structural characterization	281:311	The structural characterization of ICP-1	281:320	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	10	dep	red	457:459	arg1	test					461:464	test	461:464	test	461:464	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	4	11	theme	2.98 × 103 kDa	759:772	arg1	weight					749:754	an average molecular weight	728:754	an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°	728:813	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32686492	5	12	dep	arabinose	901:909	arg1	1→5					896:898	1→5	896:898	1→5	896:898	The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose.					
32686492	5	13	dep	acid	931:934	arg1	1→4					913:915	1→4	913:915	1→4	913:915	The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose.					
32686492	2	14	theme	structural	285:294	arg1	characterization					296:311	The structural characterization	281:311	The structural characterization of ICP-1	281:320	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	4	15	with	hetero-polysaccharide	701:721	arg1	weight					749:754	an average molecular weight	728:754	an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°	728:813	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32686492	3	16	theme	acid	517:520	arg1	bacteria					522:529	lactic acid bacteria	510:529	lactic acid bacteria	510:529	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	0	17	from	potential	42:50	arg1	Chrysanthemum					94:106	Imperial Chrysanthemum	85:106	Imperial Chrysanthemum	85:106	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	4	18	theme	molecular	739:747	arg1	weight					749:754	an average molecular weight	728:754	an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°	728:813	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32686492	1	19	theme	Sephadex	189:196	arg1	G-200					198:202	Sephadex G-200	189:202	Sephadex G-200	189:202	A novel water-soluble polysaccharide, named ICP-1, was isolated and purified by Sephadex G-200 after extracting the crude polysaccharide (ICP) from Imperial Chrysanthemum.					
32686492	0	20	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	3	21	theme	tolerance	539:547	arg1	tests					549:553	the tolerance tests	535:553	the tolerance tests of simulated gastrointestinal fluid	535:589	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	4	22	theme	average	731:737	arg1	weight					749:754	an average molecular weight	728:754	an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°	728:813	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32686492	0	23	theme	prebiotic	32:40	arg1	potential					42:50	prebiotic potential	32:50	prebiotic potential	32:50	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	5	24	theme	galacturonic	918:929	arg1	acid					931:934	(1→4) galacturonic acid	912:934	(1→4) galacturonic acid	912:934	The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose.					
32686492	1	25	theme	novel	111:115	arg1	polysaccharide					131:144	A novel water-soluble polysaccharide	109:144	A novel water-soluble polysaccharide	109:144	A novel water-soluble polysaccharide, named ICP-1, was isolated and purified by Sephadex G-200 after extracting the crude polysaccharide (ICP) from Imperial Chrysanthemum.					
32686492	2	26	theme	Smith	416:420	arg1	methods					362:368	physical and chemical methods	340:368	physical and chemical methods	340:368	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	26	theme	Smith	416:420	arg1	degradation					422:432	Smith degradation	416:432	Smith degradation	416:432	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	6	27	theme	acid	1080:1083	arg1	bacteria					1085:1092	lactic acid bacteria	1073:1092	lactic acid bacteria	1073:1092	Besides, ICP-1 can speed up the acid production of lactic acid bacteria and promote the growth and proliferation of lactic acid bacteria effectively.					
32686492	3	28	theme	lactic	510:515	arg1	bacteria					522:529	lactic acid bacteria	510:529	lactic acid bacteria	510:529	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	0	29	from	Chrysanthemum	94:106	arg1	polysaccharide					65:78	an acidic polysaccharide	55:78	an acidic polysaccharide from Imperial Chrysanthemum	55:106	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	0	29	from	Chrysanthemum	94:106	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	0	29	from	Chrysanthemum	94:106	arg1	potential					42:50	prebiotic potential	32:50	prebiotic potential	32:50	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	1	30	theme	water-soluble	117:129	arg1	polysaccharide					131:144	A novel water-soluble polysaccharide	109:144	A novel water-soluble polysaccharide	109:144	A novel water-soluble polysaccharide, named ICP-1, was isolated and purified by Sephadex G-200 after extracting the crude polysaccharide (ICP) from Imperial Chrysanthemum.					
32686492	5	31	theme	ICP-1	841:845	arg1	ICP-1					841:845	ICP-1	841:845	ICP-1	841:845	The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose.					
32686492	5	31	theme	ICP-1	841:845	arg1	residues					829:836	The glycosyl residues	816:836	The glycosyl residues of ICP-1	816:845	The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose.					
32686492	3	32	theme	acid	473:476	arg1	production					478:487	acid production	473:487	acid production	473:487	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	3	33	theme	potential	646:654	arg1	activity					624:631	the activity	620:631	the activity of prebiotic potential	620:654	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	3	34	theme	fluid	585:589	arg1	proliferation					493:505	proliferation	493:505	proliferation	493:505	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	3	34	theme	fluid	585:589	arg1	tests					549:553	the tolerance tests	535:553	the tolerance tests of simulated gastrointestinal fluid	535:589	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	3	34	theme	fluid	585:589	arg1	production					478:487	acid production	473:487	acid production	473:487	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	2	35	theme	periodate	395:403	arg1	methods					362:368	physical and chemical methods	340:368	physical and chemical methods	340:368	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	35	theme	periodate	395:403	arg1	oxidation					405:413	periodate oxidation	395:413	periodate oxidation	395:413	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	5	36	theme	glycosyl	820:827	arg1	ICP-1					841:845	ICP-1	841:845	ICP-1	841:845	The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose.					
32686492	5	36	theme	glycosyl	820:827	arg1	residues					829:836	The glycosyl residues	816:836	The glycosyl residues of ICP-1	816:845	The glycosyl residues of ICP-1 were composed of (1→), (1→4) and (1→6) glucose, (1→5) arabinose, (1→4) galacturonic acid and (1→3,6) mannose.					
32686492	1	37	theme	crude	225:229	arg1	ICP					247:249	ICP	247:249	ICP	247:249	A novel water-soluble polysaccharide, named ICP-1, was isolated and purified by Sephadex G-200 after extracting the crude polysaccharide (ICP) from Imperial Chrysanthemum.					
32686492	1	37	theme	crude	225:229	arg1	polysaccharide					231:244	the crude polysaccharide	221:244	the crude polysaccharide (ICP)	221:250	A novel water-soluble polysaccharide, named ICP-1, was isolated and purified by Sephadex G-200 after extracting the crude polysaccharide (ICP) from Imperial Chrysanthemum.					
32686492	0	38	theme	acidic	58:63	arg1	polysaccharide					65:78	an acidic polysaccharide	55:78	an acidic polysaccharide from Imperial Chrysanthemum	55:106	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	3	39	theme	simulated	558:566	arg1	fluid					585:589	simulated gastrointestinal fluid	558:589	simulated gastrointestinal fluid	558:589	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	6	40	theme	lactic	1073:1078	arg1	bacteria					1085:1092	lactic acid bacteria	1073:1092	lactic acid bacteria	1073:1092	Besides, ICP-1 can speed up the acid production of lactic acid bacteria and promote the growth and proliferation of lactic acid bacteria effectively.					
32686492	3	41	theme	gastrointestinal	568:583	arg1	fluid					585:589	simulated gastrointestinal fluid	558:589	simulated gastrointestinal fluid	558:589	Then, acid production and proliferation of lactic acid bacteria and the tolerance tests of simulated gastrointestinal fluid were measured to investigate the activity of prebiotic potential.					
32686492	2	42	theme	chemical	353:360	arg1	HPGPC					388:392	HPGPC	388:392	HPGPC	388:392	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	42	theme	chemical	353:360	arg1	degradation					422:432	Smith degradation	416:432	Smith degradation	416:432	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	42	theme	chemical	353:360	arg1	FT-IR					371:375	FT-IR	371:375	FT-IR	371:375	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	42	theme	chemical	353:360	arg1	methylation					435:445	methylation	435:445	methylation	435:445	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	42	theme	chemical	353:360	arg1	NMR					378:380	NMR	378:380	NMR	378:380	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	42	theme	chemical	353:360	arg1	oxidation					405:413	periodate oxidation	395:413	periodate oxidation	395:413	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	42	theme	chemical	353:360	arg1	methods					362:368	physical and chemical methods	340:368	physical and chemical methods	340:368	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	42	theme	chemical	353:360	arg1	red					457:459	red	457:459	red	457:459	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	42	theme	chemical	353:360	arg1	SEM					383:385	SEM	383:385	SEM	383:385	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	4	43	theme	rotation	797:804	arg1	weight					749:754	an average molecular weight	728:754	an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°	728:813	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32686492	6	44	theme	acid	1015:1018	arg1	bacteria					1020:1027	lactic acid bacteria	1008:1027	lactic acid bacteria	1008:1027	Besides, ICP-1 can speed up the acid production of lactic acid bacteria and promote the growth and proliferation of lactic acid bacteria effectively.					
32686492	6	45	theme	lactic	1008:1013	arg1	bacteria					1020:1027	lactic acid bacteria	1008:1027	lactic acid bacteria	1008:1027	Besides, ICP-1 can speed up the acid production of lactic acid bacteria and promote the growth and proliferation of lactic acid bacteria effectively.					
32686492	4	46	theme	specific	780:787	arg1	rotation					797:804	a specific optical rotation	778:804	a specific optical rotation of +155°	778:813	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32686492	4	47	theme	optical	789:795	arg1	rotation					797:804	a specific optical rotation	778:804	a specific optical rotation of +155°	778:813	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32686492	2	48	theme	physical	340:347	arg1	HPGPC					388:392	HPGPC	388:392	HPGPC	388:392	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	48	theme	physical	340:347	arg1	degradation					422:432	Smith degradation	416:432	Smith degradation	416:432	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	48	theme	physical	340:347	arg1	FT-IR					371:375	FT-IR	371:375	FT-IR	371:375	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	48	theme	physical	340:347	arg1	methylation					435:445	methylation	435:445	methylation	435:445	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	48	theme	physical	340:347	arg1	NMR					378:380	NMR	378:380	NMR	378:380	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	48	theme	physical	340:347	arg1	oxidation					405:413	periodate oxidation	395:413	periodate oxidation	395:413	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	48	theme	physical	340:347	arg1	methods					362:368	physical and chemical methods	340:368	physical and chemical methods	340:368	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	48	theme	physical	340:347	arg1	red					457:459	red	457:459	red	457:459	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	2	48	theme	physical	340:347	arg1	SEM					383:385	SEM	383:385	SEM	383:385	The structural characterization of ICP-1 was determined by physical and chemical methods, FT-IR, NMR, SEM, HPGPC, periodate oxidation, Smith degradation, methylation and Congo red test.					
32686492	4	49	theme	+155°	809:813	arg1	rotation					797:804	a specific optical rotation	778:804	a specific optical rotation of +155°	778:813	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32686492	4	49	theme	+155°	809:813	arg1	2.98 × 103 kDa					759:772	2.98 × 103 kDa	759:772	2.98 × 103 kDa	759:772	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32686492	0	50	theme	polysaccharide	65:78	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	0	50	theme	polysaccharide	65:78	arg1	potential					42:50	prebiotic potential	32:50	prebiotic potential	32:50	Structural characterization and prebiotic potential of an acidic polysaccharide from Imperial Chrysanthemum.					
32686492	6	51	theme	bacteria	1085:1092	arg1	growth					1045:1050	growth	1045:1050	growth	1045:1050	Besides, ICP-1 can speed up the acid production of lactic acid bacteria and promote the growth and proliferation of lactic acid bacteria effectively.					
32686492	6	51	theme	bacteria	1085:1092	arg1	proliferation					1056:1068	proliferation	1056:1068	proliferation	1056:1068	Besides, ICP-1 can speed up the acid production of lactic acid bacteria and promote the growth and proliferation of lactic acid bacteria effectively.					
32686492	4	52	theme	acidic	694:699	arg1	hetero-polysaccharide					701:721	an acidic hetero-polysaccharide	691:721	an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°	691:813	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32686492	4	52	theme	acidic	694:699	arg1	ICP-1					681:685	ICP-1	681:685	ICP-1	681:685	The results showed that ICP-1 was an acidic hetero-polysaccharide with an average molecular weight of 2.98 × 103 kDa and a specific optical rotation of +155°.					
32960608	0	0	theme	Material	121:128	arg1	Characterization					71:86	the Direct Characterization	60:86	the Direct Characterization of Biomineral-Associated Organic Material	60:128	Surface-Enhanced Raman Scattering Microspectroscopy Enables the Direct Characterization of Biomineral-Associated Organic Material on Single Calcareous Microskeletons.					
32960608	3	1	theme	calcifying	378:387	arg1	organisms					389:397	Many calcifying organisms	373:397	Many calcifying organisms	373:397	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	1	2	theme	inorganic	208:216	arg1	components					230:239	inorganic and organic components	208:239	inorganic and organic components	208:239	Biominerals are composite materials with inorganic and organic components.					
32960608	0	3	theme	Organic	113:119	arg1	Material					121:128	Biomineral-Associated Organic Material	91:128	Biomineral-Associated Organic Material	91:128	Surface-Enhanced Raman Scattering Microspectroscopy Enables the Direct Characterization of Biomineral-Associated Organic Material on Single Calcareous Microskeletons.					
32960608	6	4	theme	species	1119:1125	arg1	study					1063:1067	the study	1059:1067	the study of micro/nannofossil assemblages and uncultivated species	1059:1125	This approach may find applications in the study of micro/nannofossil assemblages and uncultivated species, providing evolutionary insights into the macromolecular repertoire involved in biomineralization.					
32960608	0	5	theme	Single	133:138	arg1	Microskeletons					151:164	Single Calcareous Microskeletons	133:164	Single Calcareous Microskeletons	133:164	Surface-Enhanced Raman Scattering Microspectroscopy Enables the Direct Characterization of Biomineral-Associated Organic Material on Single Calcareous Microskeletons.					
32960608	1	6	with	materials	193:201	arg1	components					230:239	inorganic and organic components	208:239	inorganic and organic components	208:239	Biominerals are composite materials with inorganic and organic components.					
32960608	5	7	theme	heterogeneous	976:988	arg1	assemblage					1008:1017	a heterogeneous micro/nannofossil assemblage	974:1017	a heterogeneous micro/nannofossil assemblage	974:1017	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	4	8	theme	organic	662:668	arg1	material					670:677	the biomineral-associated organic material	636:677	the biomineral-associated organic material from single particles within such assemblages	636:723	There is currently no method for characterizing the biomineral-associated organic material from single particles within such assemblages, so its compositional diversity is unknown.					
32960608	5	9	theme	micro/nannofossil	990:1006	arg1	assemblage					1008:1017	a heterogeneous micro/nannofossil assemblage	974:1017	a heterogeneous micro/nannofossil assemblage	974:1017	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	4	10	theme	biomineral-associated	640:660	arg1	material					670:677	the biomineral-associated organic material	636:677	the biomineral-associated organic material from single particles within such assemblages	636:723	There is currently no method for characterizing the biomineral-associated organic material from single particles within such assemblages, so its compositional diversity is unknown.					
32960608	5	11	theme	surface-enhanced	813:828	arg1	microspectroscopy					847:863	surface-enhanced Raman scattering microspectroscopy	813:863	surface-enhanced Raman scattering microspectroscopy	813:863	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	1	12	theme	organic	222:228	arg1	components					230:239	inorganic and organic components	208:239	inorganic and organic components	208:239	Biominerals are composite materials with inorganic and organic components.					
32960608	0	13	theme	Calcareous	140:149	arg1	Microskeletons					151:164	Single Calcareous Microskeletons	133:164	Single Calcareous Microskeletons	133:164	Surface-Enhanced Raman Scattering Microspectroscopy Enables the Direct Characterization of Biomineral-Associated Organic Material on Single Calcareous Microskeletons.					
32960608	3	14	theme	environmental	501:513	arg1	materials					443:451	the only materials	434:451	the only materials available for their study	434:477	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	3	14	theme	environmental	501:513	arg1	samples					515:521	complex environmental samples	493:521	complex environmental samples in which the organism of interest may only be a minor component	493:585	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	0	15	theme	Raman	17:21	arg1	Microspectroscopy					34:50	Surface-Enhanced Raman Scattering Microspectroscopy	0:50	Surface-Enhanced Raman Scattering Microspectroscopy	0:50	Surface-Enhanced Raman Scattering Microspectroscopy Enables the Direct Characterization of Biomineral-Associated Organic Material on Single Calcareous Microskeletons.					
32960608	4	16	from	particles	691:699	arg1	material					670:677	the biomineral-associated organic material	636:677	the biomineral-associated organic material from single particles within such assemblages	636:723	There is currently no method for characterizing the biomineral-associated organic material from single particles within such assemblages, so its compositional diversity is unknown.					
32960608	0	17	theme	Surface-Enhanced	0:15	arg1	Microspectroscopy					34:50	Surface-Enhanced Raman Scattering Microspectroscopy	0:50	Surface-Enhanced Raman Scattering Microspectroscopy	0:50	Surface-Enhanced Raman Scattering Microspectroscopy Enables the Direct Characterization of Biomineral-Associated Organic Material on Single Calcareous Microskeletons.					
32960608	6	18	theme	assemblages	1090:1100	arg1	study					1063:1067	the study	1059:1067	the study of micro/nannofossil assemblages and uncultivated species	1059:1125	This approach may find applications in the study of micro/nannofossil assemblages and uncultivated species, providing evolutionary insights into the macromolecular repertoire involved in biomineralization.					
32960608	5	19	theme	single-particle	949:963	arg1	level					965:969	the single-particle level	945:969	the single-particle level in a heterogeneous micro/nannofossil assemblage	945:1017	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	4	20	theme	compositional	733:745	arg1	diversity					747:755	its compositional diversity	729:755	its compositional diversity	729:755	There is currently no method for characterizing the biomineral-associated organic material from single particles within such assemblages, so its compositional diversity is unknown.					
32960608	6	21	theme	micro/nannofossil	1072:1088	arg1	assemblages					1090:1100	micro/nannofossil assemblages	1072:1100	micro/nannofossil assemblages	1072:1100	This approach may find applications in the study of micro/nannofossil assemblages and uncultivated species, providing evolutionary insights into the macromolecular repertoire involved in biomineralization.					
32960608	4	22	theme	such	708:711	arg1	assemblages					713:723	such assemblages	708:723	such assemblages	708:723	There is currently no method for characterizing the biomineral-associated organic material from single particles within such assemblages, so its compositional diversity is unknown.					
32960608	0	23	theme	Scattering	23:32	arg1	Microspectroscopy					34:50	Surface-Enhanced Raman Scattering Microspectroscopy	0:50	Surface-Enhanced Raman Scattering Microspectroscopy	0:50	Surface-Enhanced Raman Scattering Microspectroscopy Enables the Direct Characterization of Biomineral-Associated Organic Material on Single Calcareous Microskeletons.					
32960608	2	24	theme	past	358:361	arg1	climates					363:370	past climates	358:370	past climates	358:370	The latter provide insights into how organisms control mineralization and, if derived from micro/nannofossils, into past climates.					
32960608	5	25	theme	organic	927:933	arg1	matter					935:940	fossil organic matter	920:940	fossil organic matter	920:940	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	6	26	theme	macromolecular	1169:1182	arg1	repertoire					1184:1193	the macromolecular repertoire	1165:1193	the macromolecular repertoire involved in biomineralization	1165:1223	This approach may find applications in the study of micro/nannofossil assemblages and uncultivated species, providing evolutionary insights into the macromolecular repertoire involved in biomineralization.					
32960608	5	27	theme	scattering	836:845	arg1	microspectroscopy					847:863	surface-enhanced Raman scattering microspectroscopy	813:863	surface-enhanced Raman scattering microspectroscopy	813:863	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	5	28	theme	matter	935:940	arg1	composition					905:915	composition	905:915	composition	905:915	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	5	28	theme	matter	935:940	arg1	origin					894:899	origin	894:899	origin	894:899	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	3	29	theme	complex	493:499	arg1	materials					443:451	the only materials	434:451	the only materials available for their study	434:477	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	3	29	theme	complex	493:499	arg1	samples					515:521	complex environmental samples	493:521	complex environmental samples in which the organism of interest may only be a minor component	493:585	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	5	30	used	used	872:875	arg2	microspectroscopy					847:863	surface-enhanced Raman scattering microspectroscopy	813:863	surface-enhanced Raman scattering microspectroscopy	813:863	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	5	31	theme	Raman	830:834	arg1	microspectroscopy					847:863	surface-enhanced Raman scattering microspectroscopy	813:863	surface-enhanced Raman scattering microspectroscopy	813:863	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	3	32	theme	available	453:461	arg1	materials					443:451	the only materials	434:451	the only materials available for their study	434:477	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	3	32	theme	available	453:461	arg1	samples					515:521	complex environmental samples	493:521	complex environmental samples in which the organism of interest may only be a minor component	493:585	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	3	33	theme	interest	548:555	arg1	organism					536:543	the organism	532:543	the organism of interest	532:555	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	3	33	theme	interest	548:555	arg1	component					577:585	a minor component	569:585	a minor component	569:585	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	0	34	theme	Direct	64:69	arg1	Characterization					71:86	the Direct Characterization	60:86	the Direct Characterization of Biomineral-Associated Organic Material	60:128	Surface-Enhanced Raman Scattering Microspectroscopy Enables the Direct Characterization of Biomineral-Associated Organic Material on Single Calcareous Microskeletons.					
32960608	3	35	theme	Many	373:376	arg1	organisms					389:397	Many calcifying organisms	373:397	Many calcifying organisms	373:397	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	5	36	from	level	965:969	arg1	assemblage					1008:1017	a heterogeneous micro/nannofossil assemblage	974:1017	a heterogeneous micro/nannofossil assemblage	974:1017	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	1	37	theme	composite	183:191	arg1	Biominerals					167:177	Biominerals	167:177	Biominerals	167:177	Biominerals are composite materials with inorganic and organic components.					
32960608	1	37	theme	composite	183:191	arg1	materials					193:201	composite materials	183:201	composite materials with inorganic and organic components	183:239	Biominerals are composite materials with inorganic and organic components.					
32960608	0	38	theme	Biomineral-Associated	91:111	arg1	Material					121:128	Biomineral-Associated Organic Material	91:128	Biomineral-Associated Organic Material	91:128	Surface-Enhanced Raman Scattering Microspectroscopy Enables the Direct Characterization of Biomineral-Associated Organic Material on Single Calcareous Microskeletons.					
32960608	5	39	theme	fossil	920:925	arg1	matter					935:940	fossil organic matter	920:940	fossil organic matter	920:940	Focusing on coccoliths, we demonstrate that surface-enhanced Raman scattering microspectroscopy can be used to determine the origin and composition of fossil organic matter at the single-particle level in a heterogeneous micro/nannofossil assemblage.					
32960608	3	40	theme	only	438:441	arg1	materials					443:451	the only materials	434:451	the only materials available for their study	434:477	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	3	40	theme	only	438:441	arg1	samples					515:521	complex environmental samples	493:521	complex environmental samples in which the organism of interest may only be a minor component	493:585	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	6	41	theme	evolutionary	1138:1149	arg1	insights					1151:1158	evolutionary insights	1138:1158	evolutionary insights into the macromolecular repertoire involved in biomineralization	1138:1223	This approach may find applications in the study of micro/nannofossil assemblages and uncultivated species, providing evolutionary insights into the macromolecular repertoire involved in biomineralization.					
32960608	6	42	theme	uncultivated	1106:1117	arg1	species					1119:1125	uncultivated species	1106:1125	uncultivated species	1106:1125	This approach may find applications in the study of micro/nannofossil assemblages and uncultivated species, providing evolutionary insights into the macromolecular repertoire involved in biomineralization.					
32960608	3	43	theme	minor	571:575	arg1	component					577:585	a minor component	569:585	a minor component	569:585	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	3	43	theme	minor	571:575	arg1	organism					536:543	the organism	532:543	the organism of interest	532:555	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	3	44	from	component	577:585	arg1	materials					443:451	the only materials	434:451	the only materials available for their study	434:477	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	3	44	from	component	577:585	arg1	samples					515:521	complex environmental samples	493:521	complex environmental samples in which the organism of interest may only be a minor component	493:585	Many calcifying organisms cannot be cultured or are extinct; the only materials available for their study are therefore complex environmental samples in which the organism of interest may only be a minor component.					
32960608	4	45	theme	single	684:689	arg1	particles					691:699	single particles	684:699	single particles within such assemblages	684:723	There is currently no method for characterizing the biomineral-associated organic material from single particles within such assemblages, so its compositional diversity is unknown.					
31999773	7	0	theme	abundant	943:950	arg1	ones					952:955	the most abundant ones	934:955	the di and tri-decameric cylinders the most abundant ones	899:955	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	10	1	theme	O-linked	1190:1197	arg1	glycosylation					1199:1211	O-linked glycosylation	1190:1211	O-linked glycosylation	1190:1211	The molecule lacks O-linked glycosylation but presents the T-antigen (Gal-β1,3-GalNAc).					
31999773	8	2	theme	N-linked	1043:1050	arg1	glycans					1052:1058	complex-type N-linked glycans	1030:1058	complex-type N-linked glycans	1030:1058	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	11	3	theme	other	1345:1349	arg1	hemocyanins					1361:1371	other gastropod hemocyanins	1345:1371	other gastropod hemocyanins	1345:1371	Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins.					
31999773	8	4	theme	high	985:988	arg1	mannose					990:996	high mannose	985:996	high mannose	985:996	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	12	5	theme	first	1513:1517	arg1	This					1470:1473	This	1470:1473	This	1470:1473	This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.					
31999773	12	5	theme	first	1513:1517	arg1	study					1534:1538	the first gene structure study	1509:1538	the first gene structure study of a Caenogastropoda hemocyanin	1509:1570	This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.					
31999773	12	6	theme	structure	1524:1532	arg1	This					1470:1473	This	1470:1473	This	1470:1473	This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.					
31999773	12	6	theme	structure	1524:1532	arg1	study					1534:1538	the first gene structure study	1509:1538	the first gene structure study of a Caenogastropoda hemocyanin	1509:1570	This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.					
31999773	11	7	theme	differences	1429:1439	arg1	presence					1391:1398	the presence	1387:1398	the presence of clade-specific structural differences among gastropod hemocyanins	1387:1467	Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins.					
31999773	8	8	theme	hybrid-type	1002:1012	arg1	structures					1014:1023	hybrid-type structures	1002:1023	hybrid-type structures	1002:1023	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	11	9	theme	polyclonal	1277:1286	arg1	antibody					1288:1295	an anti-PcH polyclonal antibody	1265:1295	an anti-PcH polyclonal antibody	1265:1295	Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins.					
31999773	3	10	with	contrast	378:385	arg1	gastropods					398:407	other gastropods	392:407	other gastropods that usually have 2 or 3	392:432	Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3.					
31999773	4	11	theme	limpet-type	472:482	arg1	architecture					495:506	the typical Keyhole limpet-type hemocyanin architecture	452:506	the typical Keyhole limpet-type hemocyanin architecture	452:506	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	3	12	theme	subunit	357:363	arg1	isoforms					365:372	4 PcH subunit isoforms	351:372	4 PcH subunit isoforms	351:372	Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3.					
31999773	4	13	theme	Keyhole	464:470	arg1	architecture					495:506	the typical Keyhole limpet-type hemocyanin architecture	452:506	the typical Keyhole limpet-type hemocyanin architecture	452:506	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	5	14	theme	FUs	617:619	arg1	regions					628:634	eight FUs coding regions	611:634	eight FUs coding regions	611:634	Correspondingly, genes are organized in eight FUs coding regions.					
31999773	3	15	contain	have	422:425	arg2	2					427:427	2	427:427	2	427:427	Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3.					
31999773	3	15	contain	have	422:425	arg1	gastropods					398:407	other gastropods	392:407	other gastropods that usually have 2 or 3	392:432	Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3.					
31999773	3	15	contain	have	422:425	arg2	3					432:432	3	432:432	3	432:432	Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3.					
31999773	11	16	theme	gastropod	1351:1359	arg1	hemocyanins					1361:1371	other gastropod hemocyanins	1345:1371	other gastropod hemocyanins	1345:1371	Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins.					
31999773	5	17	theme	coding	621:626	arg1	regions					628:634	eight FUs coding regions	611:634	eight FUs coding regions	611:634	Correspondingly, genes are organized in eight FUs coding regions.					
31999773	12	18	theme	Caenogastropoda	1545:1559	arg1	hemocyanin					1561:1570	a Caenogastropoda hemocyanin	1543:1570	a Caenogastropoda hemocyanin	1543:1570	This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.					
31999773	11	19	theme	gastropod	1447:1455	arg1	hemocyanins					1457:1467	gastropod hemocyanins	1447:1467	gastropod hemocyanins	1447:1467	Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins.					
31999773	11	20	theme	clade-specific	1403:1416	arg1	differences					1429:1439	clade-specific structural differences	1403:1439	clade-specific structural differences among gastropod hemocyanins	1403:1467	Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins.					
31999773	3	21	theme	other	392:396	arg1	gastropods					398:407	other gastropods	392:407	other gastropods that usually have 2 or 3	392:432	Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3.					
31999773	4	22	theme	hemocyanin	484:493	arg1	architecture					495:506	the typical Keyhole limpet-type hemocyanin architecture	452:506	the typical Keyhole limpet-type hemocyanin architecture	452:506	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	8	23	theme	complex-type	1030:1041	arg1	glycans					1052:1058	complex-type N-linked glycans	1030:1058	complex-type N-linked glycans	1030:1058	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	7	24	theme	di	903:904	arg1	cylinders					924:932	the di and tri-decameric cylinders	899:932	the di and tri-decameric cylinders the most abundant ones	899:955	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	1	25	theme	Structural	112:121	arg1	knowledge					123:131	Structural knowledge	112:131	Structural knowledge of gastropod hemocyanins	112:156	Structural knowledge of gastropod hemocyanins is scarce.					
31999773	8	26	link	N-linked	1043:1050	arg1	glycans					1052:1058	complex-type N-linked glycans	1030:1058	complex-type N-linked glycans	1030:1058	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	7	27	theme	cylindrical	796:806	arg1	structure					808:816	a cylindrical structure	794:816	a cylindrical structure organized in di, tri and tetra-decamers	794:856	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	11	28	theme	anti-PcH	1268:1275	arg1	antibody					1288:1295	an anti-PcH polyclonal antibody	1265:1295	an anti-PcH polyclonal antibody	1265:1295	Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins.					
31999773	0	29	theme	caenogastropod	18:31	arg1	canaliculata					41:52	the caenogastropod Pomacea canaliculata	14:52	the caenogastropod Pomacea canaliculata	14:52	Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades.					
31999773	4	30	theme	units	558:562	arg1	string					522:527	a string	520:527	a string of eight globular functional units (FUs)	520:568	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	7	31	theme	tri-decameric	910:922	arg1	cylinders					924:932	the di and tri-decameric cylinders	899:932	the di and tri-decameric cylinders the most abundant ones	899:955	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	6	32	from	FUs	641:643	arg1	genes					654:658	the 4 genes	648:658	the 4 genes	648:658	All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods.					
31999773	7	33	theme	microscopy	762:771	arg1	images					773:778	Transmission electron microscopy images	740:778	Transmission electron microscopy images of PcH	740:785	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	3	34	theme	proteomic	305:313	arg1	approach					327:334	a proteomic and genomic approach	303:334	approach	327:334	Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3.					
31999773	1	35	theme	gastropod	136:144	arg1	hemocyanins					146:156	gastropod hemocyanins	136:156	gastropod hemocyanins	136:156	Structural knowledge of gastropod hemocyanins is scarce.					
31999773	0	36	theme	canaliculata	41:52	arg1	Hemocyanin					0:9	Hemocyanin	0:9	Hemocyanin of the caenogastropod Pomacea canaliculata	0:52	Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades.					
31999773	2	37	theme	Pomacea	267:273	arg1	PcH					289:291	PcH	289:291	PcH	289:291	To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH).					
31999773	2	37	theme	Pomacea	267:273	arg1	caenogastropod					251:264	a caenogastropod	249:264	a caenogastropod	249:264	To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH).					
31999773	2	37	theme	Pomacea	267:273	arg1	canaliculata					275:286	Pomacea canaliculata	267:286	Pomacea canaliculata (PcH)	267:292	To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH).					
31999773	0	38	theme	Pomacea	33:39	arg1	canaliculata					41:52	the caenogastropod Pomacea canaliculata	14:52	the caenogastropod Pomacea canaliculata	14:52	Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades.					
31999773	7	39	theme	Transmission	740:751	arg1	microscopy					762:771	Transmission electron microscopy	740:771	Transmission electron microscopy images of PcH	740:785	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	4	40	theme	typical	456:462	arg1	architecture					495:506	the typical Keyhole limpet-type hemocyanin architecture	452:506	the typical Keyhole limpet-type hemocyanin architecture	452:506	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	11	41	theme	structural	1418:1427	arg1	differences					1429:1439	clade-specific structural differences	1403:1439	clade-specific structural differences among gastropod hemocyanins	1403:1467	Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins.					
31999773	6	42	theme	non-	718:721	arg1	caenogastropods					723:737	non- caenogastropods	718:737	non- caenogastropods	718:737	All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods.					
31999773	4	43	contain	has	448:450	arg2	architecture					495:506	the typical Keyhole limpet-type hemocyanin architecture	452:506	the typical Keyhole limpet-type hemocyanin architecture	452:506	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	4	43	contain	has	448:450	arg1	isoform					440:446	Each isoform	435:446	Each isoform	435:446	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	8	44	gly	N-glycosylated	965:978	arg1	PcH					958:960	PcH	958:960	PcH	958:960	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	7	45	theme	electron	753:760	arg1	microscopy					762:771	Transmission electron microscopy	740:771	Transmission electron microscopy images of PcH	740:785	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	7	46	theme	PcH	783:785	arg1	images					773:778	Transmission electron microscopy images	740:778	Transmission electron microscopy images of PcH	740:785	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	2	47	theme	caenogastropod	251:264	arg1	hemocyanin					235:244	the hemocyanin	231:244	the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH)	231:292	To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH).					
31999773	9	48	theme	Terminal	1090:1097	arg1	residues					1110:1117	Terminal β-N-GlcNAc residues	1090:1117	Terminal β-N-GlcNAc residues	1090:1117	Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present.					
31999773	1	49	theme	hemocyanins	146:156	arg1	knowledge					123:131	Structural knowledge	112:131	Structural knowledge of gastropod hemocyanins	112:156	Structural knowledge of gastropod hemocyanins is scarce.					
31999773	6	50	located	found	709:713	arg2	exon					689:692	more than one exon	675:692	more than one exon	675:692	All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods.					
31999773	6	50	located	found	709:713	arg1	caenogastropods					723:737	non- caenogastropods	718:737	non- caenogastropods	718:737	All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods.					
31999773	6	50	located	found	709:713	arg2	feature					697:703	a feature	695:703	a feature not found in non- caenogastropods	695:737	All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods.					
31999773	4	51	theme	functional	547:556	arg1	FUs					565:567	FUs	565:567	FUs	565:567	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	4	51	theme	functional	547:556	arg1	units					558:562	eight globular functional units	532:562	eight globular functional units (FUs)	532:568	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	9	52	theme	β-N-GlcNAc	1099:1108	arg1	residues					1110:1117	Terminal β-N-GlcNAc residues	1090:1117	Terminal β-N-GlcNAc residues	1090:1117	Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present.					
31999773	12	53	theme	gene	1519:1522	arg1	This					1470:1473	This	1470:1473	This	1470:1473	This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.					
31999773	12	53	theme	gene	1519:1522	arg1	study					1534:1538	the first gene structure study	1509:1538	the first gene structure study of a Caenogastropoda hemocyanin	1509:1570	This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.					
31999773	7	54	theme	collar	875:880	arg1	structure					882:890	an internal collar structure	863:890	an internal collar structure	863:890	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	4	55	theme	globular	538:545	arg1	FUs					565:567	FUs	565:567	FUs	565:567	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	4	55	theme	globular	538:545	arg1	units					558:562	eight globular functional units	532:562	eight globular functional units (FUs)	532:568	Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs).					
31999773	8	56	theme	acid	1084:1087	arg1	absence					1066:1072	absence	1066:1072	absence of sialic acid	1066:1087	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	8	57	theme	sialic	1077:1082	arg1	acid					1084:1087	sialic acid	1077:1087	sialic acid	1077:1087	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	12	58	theme	hemocyanin	1561:1570	arg1	This					1470:1473	This	1470:1473	This	1470:1473	This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.					
31999773	12	58	theme	hemocyanin	1561:1570	arg1	study					1534:1538	the first gene structure study	1509:1538	the first gene structure study of a Caenogastropoda hemocyanin	1509:1570	This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.					
31999773	3	59	theme	genomic	319:325	arg1	approach					327:334	a proteomic and genomic approach	303:334	approach	327:334	Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3.					
31999773	7	60	dep	cylinders	924:932	arg1	ones					952:955	the most abundant ones	934:955	the di and tri-decameric cylinders the most abundant ones	899:955	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	2	61	dep	studied	223:229	arg1	understand					179:188	understand	179:188	To better understand their evolution and diversity	169:218	To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH).					
31999773	0	62	theme	gastropod	94:102	arg1	clades					104:109	gastropod clades	94:109	gastropod clades	94:109	Hemocyanin of the caenogastropod Pomacea canaliculata exhibits evolutionary differences among gastropod clades.					
31999773	10	63	link	O-linked	1190:1197	arg1	glycosylation					1199:1211	O-linked glycosylation	1190:1211	O-linked glycosylation	1190:1211	The molecule lacks O-linked glycosylation but presents the T-antigen (Gal-β1,3-GalNAc).					
31999773	9	64	theme	nonreducing	1123:1133	arg1	α-GalNAc					1144:1151	nonreducing terminal α-GalNAc	1123:1151	nonreducing terminal α-GalNAc	1123:1151	Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present.					
31999773	7	65	theme	internal	866:873	arg1	structure					882:890	an internal collar structure	863:890	an internal collar structure	863:890	Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones.					
31999773	3	66	theme	PcH	353:355	arg1	isoforms					365:372	4 PcH subunit isoforms	351:372	4 PcH subunit isoforms	351:372	Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3.					
31999773	9	67	theme	terminal	1135:1142	arg1	α-GalNAc					1144:1151	nonreducing terminal α-GalNAc	1123:1151	nonreducing terminal α-GalNAc	1123:1151	Terminal β-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present.					
31999773	8	68	with	N-glycosylated	965:978	arg1	structures					1014:1023	hybrid-type structures	1002:1023	hybrid-type structures	1002:1023	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	8	68	with	N-glycosylated	965:978	arg1	mannose					990:996	high mannose	985:996	high mannose	985:996	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	8	68	with	N-glycosylated	965:978	arg1	absence					1066:1072	absence	1066:1072	absence of sialic acid	1066:1087	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
31999773	8	68	with	N-glycosylated	965:978	arg1	glycans					1052:1058	complex-type N-linked glycans	1030:1058	complex-type N-linked glycans	1030:1058	PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid.					
34893224	6	0	gly	glycosylation	862:874	arg2	sequons					876:882	routine and unreported glycosylation sequons	839:882	routine and unreported glycosylation sequons	839:882	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	5	1	theme	carrier	721:727	arg1	protein					729:735	carrier protein	721:735	carrier protein	721:735	The optimal expression of oligosaccharyltransferase and carrier protein further improved the titer.					
34893224	4	2	theme	glyco-optimized	571:585	arg1	strain					595:600	glyco-optimized chassis strain	571:600	glyco-optimized chassis strain	571:600	Specifically, OPS and OPS-based glycoprotein were synthesized in glyco-optimized chassis strain, when compared to the unmeasurable level of the parent strain.					
34893224	5	3	theme	protein	729:735	arg1	expression					677:686	The optimal expression	665:686	The optimal expression of oligosaccharyltransferase and carrier protein	665:735	The optimal expression of oligosaccharyltransferase and carrier protein further improved the titer.					
34893224	8	4	theme	plug-and-play	1088:1100	arg1	suitable					1130:1137	suitable	1130:1137	suitable	1130:1137	The plug-and-play glyco-optimized platform is suitable for bioconjugate synthesis, thus providing a potential platform for future medical applications.					
34893224	8	4	theme	plug-and-play	1088:1100	arg1	platform					1118:1125	The plug-and-play glyco-optimized platform	1084:1125	The plug-and-play glyco-optimized platform	1084:1125	The plug-and-play glyco-optimized platform is suitable for bioconjugate synthesis, thus providing a potential platform for future medical applications.					
34893224	3	5	theme	O1-specific	385:395	arg1	strain					421:426	an O1-specific glyco-optimized chassis strain	382:426	an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen	382:476	Herein, we designed an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen and OPS-based bioconjugate.					
34893224	4	6	theme	parent	650:655	arg1	strain					657:662	the parent strain	646:662	the parent strain	646:662	Specifically, OPS and OPS-based glycoprotein were synthesized in glyco-optimized chassis strain, when compared to the unmeasurable level of the parent strain.					
34893224	3	7	theme	glyco-optimized	397:411	arg1	strain					421:426	an O1-specific glyco-optimized chassis strain	382:426	an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen	382:476	Herein, we designed an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen and OPS-based bioconjugate.					
34893224	0	8	from	vaccine	68:74	arg1	coli					93:96	engineered E. coli	79:96	engineered E. coli	79:96	Development of an O-polysaccharide based recombinant glycoconjugate vaccine in engineered E. coli against ExPEC O1.					
34893224	2	9	theme	promising	316:324	arg1	measure					326:332	a promising measure	314:332	a promising measure to reduce ExPEC infections	314:359	Glycoconjugate vaccine represents a promising measure to reduce ExPEC infections.					
34893224	6	10	theme	correct	792:798	arg1	structure					800:808	the correct structure	788:808	the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein	788:901	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	7	11	theme	purified	953:960	arg1	bioconjugate					962:973	purified bioconjugate	953:973	purified bioconjugate	953:973	Finally, purified bioconjugate stimulated mouse to generate specific IgG antibodies and protected them against virulent ExPEC O1 challenge.					
34893224	8	12	theme	potential	1184:1192	arg1	platform					1194:1201	a potential platform	1182:1201	a potential platform for future medical applications	1182:1233	The plug-and-play glyco-optimized platform is suitable for bioconjugate synthesis, thus providing a potential platform for future medical applications.					
34893224	5	13	theme	optimal	669:675	arg1	expression					677:686	The optimal expression	665:686	The optimal expression of oligosaccharyltransferase and carrier protein	665:735	The optimal expression of oligosaccharyltransferase and carrier protein further improved the titer.					
34893224	7	14	theme	specific	1004:1011	arg1	antibodies					1017:1026	specific IgG antibodies	1004:1026	specific IgG antibodies	1004:1026	Finally, purified bioconjugate stimulated mouse to generate specific IgG antibodies and protected them against virulent ExPEC O1 challenge.					
34893224	1	15	theme	identified	179:188	arg1	coli					155:158	Escherichia coli	143:158	Extraintestinal pathogenic Escherichia coli O1	116:161	Extraintestinal pathogenic Escherichia coli O1 is a frequently identified serotype that causes serious infections and is often refractory to antimicrobial therapy.					
34893224	1	15	theme	identified	179:188	arg1	serotype					190:197	a frequently identified serotype	166:197	a frequently identified serotype that causes serious infections and is often refractory to antimicrobial therapy	166:277	Extraintestinal pathogenic Escherichia coli O1 is a frequently identified serotype that causes serious infections and is often refractory to antimicrobial therapy.					
34893224	1	15	theme	identified	179:188	arg1	refractory					243:252	refractory	243:252	refractory	243:252	Extraintestinal pathogenic Escherichia coli O1 is a frequently identified serotype that causes serious infections and is often refractory to antimicrobial therapy.					
34893224	0	16	theme	glycoconjugate	53:66	arg1	vaccine					68:74	recombinant glycoconjugate vaccine	41:74	recombinant glycoconjugate vaccine in engineered E. coli against ExPEC O1	41:113	Development of an O-polysaccharide based recombinant glycoconjugate vaccine in engineered E. coli against ExPEC O1.					
34893224	6	17	theme	carrier	887:893	arg1	protein					895:901	carrier protein	887:901	carrier protein	887:901	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	4	18	theme	OPS-based	528:536	arg1	glycoprotein					538:549	OPS-based glycoprotein	528:549	OPS-based glycoprotein	528:549	Specifically, OPS and OPS-based glycoprotein were synthesized in glyco-optimized chassis strain, when compared to the unmeasurable level of the parent strain.					
34893224	5	19	theme	oligosaccharyltransferase	691:715	arg1	expression					677:686	The optimal expression	665:686	The optimal expression of oligosaccharyltransferase and carrier protein	665:735	The optimal expression of oligosaccharyltransferase and carrier protein further improved the titer.					
34893224	7	20	theme	virulent	1055:1062	arg1	challenge					1073:1081	virulent ExPEC O1 challenge	1055:1081	virulent ExPEC O1 challenge	1055:1081	Finally, purified bioconjugate stimulated mouse to generate specific IgG antibodies and protected them against virulent ExPEC O1 challenge.					
34893224	8	21	theme	bioconjugate	1143:1154	arg1	synthesis					1156:1164	bioconjugate synthesis	1143:1164	bioconjugate synthesis	1143:1164	The plug-and-play glyco-optimized platform is suitable for bioconjugate synthesis, thus providing a potential platform for future medical applications.					
34893224	2	22	theme	ExPEC	344:348	arg1	infections					350:359	ExPEC infections	344:359	ExPEC infections	344:359	Glycoconjugate vaccine represents a promising measure to reduce ExPEC infections.					
34893224	6	23	theme	MS	765:766	arg1	analysis					768:775	MS analysis	765:775	MS analysis	765:775	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	3	24	theme	antigen	470:476	arg1	manufacture					432:442	manufacture	432:442	manufacture of O-polysaccharide (OPS) antigen	432:476	Herein, we designed an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen and OPS-based bioconjugate.					
34893224	3	25	theme	chassis	413:419	arg1	strain					421:426	an O1-specific glyco-optimized chassis strain	382:426	an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen	382:476	Herein, we designed an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen and OPS-based bioconjugate.					
34893224	8	26	theme	medical	1214:1220	arg1	applications					1222:1233	future medical applications	1207:1233	future medical applications	1207:1233	The plug-and-play glyco-optimized platform is suitable for bioconjugate synthesis, thus providing a potential platform for future medical applications.					
34893224	6	27	theme	glycosylation	862:874	arg1	sequons					876:882	routine and unreported glycosylation sequons	839:882	routine and unreported glycosylation sequons	839:882	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	1	28	theme	serious	211:217	arg1	infections					219:228	serious infections	211:228	serious infections	211:228	Extraintestinal pathogenic Escherichia coli O1 is a frequently identified serotype that causes serious infections and is often refractory to antimicrobial therapy.					
34893224	4	29	theme	unmeasurable	624:635	arg1	level					637:641	the unmeasurable level	620:641	the unmeasurable level of the parent strain	620:662	Specifically, OPS and OPS-based glycoprotein were synthesized in glyco-optimized chassis strain, when compared to the unmeasurable level of the parent strain.					
34893224	8	30	theme	future	1207:1212	arg1	applications					1222:1233	future medical applications	1207:1233	future medical applications	1207:1233	The plug-and-play glyco-optimized platform is suitable for bioconjugate synthesis, thus providing a potential platform for future medical applications.					
34893224	7	31	theme	O1	1070:1071	arg1	challenge					1073:1081	virulent ExPEC O1 challenge	1055:1081	virulent ExPEC O1 challenge	1055:1081	Finally, purified bioconjugate stimulated mouse to generate specific IgG antibodies and protected them against virulent ExPEC O1 challenge.					
34893224	0	32	theme	recombinant	41:51	arg1	vaccine					68:74	recombinant glycoconjugate vaccine	41:74	recombinant glycoconjugate vaccine in engineered E. coli against ExPEC O1	41:113	Development of an O-polysaccharide based recombinant glycoconjugate vaccine in engineered E. coli against ExPEC O1.					
34893224	6	33	from	sequons	876:882	arg1	structure					800:808	the correct structure	788:808	the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein	788:901	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	6	33	from	sequons	876:882	arg1	bioconjugate					823:834	resulting bioconjugate	813:834	resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein	813:901	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	4	34	gly	glycoprotein	538:549	arg1	glycoprotein					538:549	OPS-based glycoprotein	528:549	OPS-based glycoprotein	528:549	Specifically, OPS and OPS-based glycoprotein were synthesized in glyco-optimized chassis strain, when compared to the unmeasurable level of the parent strain.					
34893224	3	35	theme	OPS	465:467	arg1	antigen					470:476	O-polysaccharide (OPS) antigen	447:476	O-polysaccharide (OPS) antigen	447:476	Herein, we designed an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen and OPS-based bioconjugate.					
34893224	0	36	theme	O-polysaccharide	18:33	arg1	Development					0:10	Development	0:10	Development of an O-polysaccharide	0:33	Development of an O-polysaccharide based recombinant glycoconjugate vaccine in engineered E. coli against ExPEC O1.					
34893224	6	37	theme	unreported	851:860	arg1	sequons					876:882	routine and unreported glycosylation sequons	839:882	routine and unreported glycosylation sequons	839:882	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	4	38	theme	chassis	587:593	arg1	strain					595:600	glyco-optimized chassis strain	571:600	glyco-optimized chassis strain	571:600	Specifically, OPS and OPS-based glycoprotein were synthesized in glyco-optimized chassis strain, when compared to the unmeasurable level of the parent strain.					
34893224	3	39	theme	O-polysaccharide	447:462	arg1	antigen					470:476	O-polysaccharide (OPS) antigen	447:476	O-polysaccharide (OPS) antigen	447:476	Herein, we designed an O1-specific glyco-optimized chassis strain for manufacture of O-polysaccharide (OPS) antigen and OPS-based bioconjugate.					
34893224	6	40	theme	routine	839:845	arg1	sequons					876:882	routine and unreported glycosylation sequons	839:882	routine and unreported glycosylation sequons	839:882	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	8	41	theme	glyco-optimized	1102:1116	arg1	suitable					1130:1137	suitable	1130:1137	suitable	1130:1137	The plug-and-play glyco-optimized platform is suitable for bioconjugate synthesis, thus providing a potential platform for future medical applications.					
34893224	8	41	theme	glyco-optimized	1102:1116	arg1	platform					1118:1125	The plug-and-play glyco-optimized platform	1084:1125	The plug-and-play glyco-optimized platform	1084:1125	The plug-and-play glyco-optimized platform is suitable for bioconjugate synthesis, thus providing a potential platform for future medical applications.					
34893224	6	42	theme	glycosylation	918:930	arg1	efficiency					932:941	a higher glycosylation efficiency	909:941	a higher glycosylation efficiency	909:941	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	2	43	theme	Glycoconjugate	280:293	arg1	vaccine					295:301	Glycoconjugate vaccine	280:301	Glycoconjugate vaccine	280:301	Glycoconjugate vaccine represents a promising measure to reduce ExPEC infections.					
34893224	6	44	theme	bioconjugate	823:834	arg1	structure					800:808	the correct structure	788:808	the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein	788:901	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	6	45	theme	protein	895:901	arg1	bioconjugate					823:834	resulting bioconjugate	813:834	resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein	813:901	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	4	46	theme	strain	657:662	arg1	level					637:641	the unmeasurable level	620:641	the unmeasurable level of the parent strain	620:662	Specifically, OPS and OPS-based glycoprotein were synthesized in glyco-optimized chassis strain, when compared to the unmeasurable level of the parent strain.					
34893224	6	47	theme	higher	911:916	arg1	efficiency					932:941	a higher glycosylation efficiency	909:941	a higher glycosylation efficiency	909:941	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	6	48	theme	resulting	813:821	arg1	bioconjugate					823:834	resulting bioconjugate	813:834	resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein	813:901	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	7	49	theme	IgG	1013:1015	arg1	antibodies					1017:1026	specific IgG antibodies	1004:1026	specific IgG antibodies	1004:1026	Finally, purified bioconjugate stimulated mouse to generate specific IgG antibodies and protected them against virulent ExPEC O1 challenge.					
34893224	6	50	from	structure	800:808	arg1	sequons					876:882	routine and unreported glycosylation sequons	839:882	routine and unreported glycosylation sequons	839:882	MS analysis elucidated the correct structure of resulting bioconjugate at routine and unreported glycosylation sequons of carrier protein, with a higher glycosylation efficiency.					
34893224	1	51	theme	antimicrobial	257:269	arg1	therapy					271:277	antimicrobial therapy	257:277	antimicrobial therapy	257:277	Extraintestinal pathogenic Escherichia coli O1 is a frequently identified serotype that causes serious infections and is often refractory to antimicrobial therapy.					
34668723	9	0	theme	Salt	1517:1520	arg1	stress					1522:1527	Salt stress	1517:1527	Salt stress	1517:1527	Salt stress induced the incorporation of β-GlcNAc anomers and drastically increased the vancomycin MIC for S. aureus VC40R.					
34668723	8	1	theme	α-glycosylation	1495:1509	arg1	peak					1511:1514	only the α-glycosylation peak	1486:1514	only the α-glycosylation peak	1486:1514	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	12	2	with	Infections	1939:1948	arg1	Staphylococcus					1977:1990	Staphylococcus	1977:1990	Staphylococcus	1977:1990	IMPORTANCE Infections with methicillin-resistant Staphylococcus aureus are commonly treated with vancomycin.					
34668723	2	3	theme	underlying	353:362	arg1	mechanisms					364:373	the underlying mechanisms	349:373	the underlying mechanisms	349:373	Due to the rapid evolution of antibiotic resistance that leads to treatment failure, it is important to understand the underlying mechanisms.					
34668723	17	4	from	sugars	2733:2738	arg1	β-conformation					2747:2760	the β-conformation	2743:2760	the β-conformation	2743:2760	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	6	5	theme	aureus	1088:1093	arg1	VC40					1100:1103	VC40	1100:1103	VC40	1100:1103	Both S. aureus VC40 and S. aureus VraS(VC40) displayed reduced autolysis and an altered autolysin profile in a zymogram.					
34668723	6	5	theme	aureus	1088:1093	arg1	VraS					1095:1098	Both S. aureus VC40 and S. aureus VraS	1061:1098	Both S. aureus VC40 and S. aureus VraS(VC40)	1061:1104	Both S. aureus VC40 and S. aureus VraS(VC40) displayed reduced autolysis and an altered autolysin profile in a zymogram.					
34668723	18	6	from	proportion	2796:2805	arg1	isolate					2856:2862	a closely related isolate	2838:2862	a closely related isolate	2838:2862	By experimentally increasing the proportion of β-N-acetyl-d-glucosamine in a closely related isolate through the induction of salt stress, we could show that the β-conformation of the sugars plays a vital role in the resistance of S. aureus VC40.					
34668723	14	7	theme	aureus	2264:2269	arg1	strain					2253:2258	a laboratory-generated strain	2230:2258	a laboratory-generated strain	2230:2258	We set out to characterize the mechanisms leading to decreased vancomycin susceptibility in a laboratory-generated strain, S. aureus VC40.					
34668723	14	7	theme	aureus	2264:2269	arg1	VC40					2271:2274	S. aureus VC40	2261:2274	S. aureus VC40	2261:2274	We set out to characterize the mechanisms leading to decreased vancomycin susceptibility in a laboratory-generated strain, S. aureus VC40.					
34668723	11	8	theme	teichoic	1857:1864	arg1	acids					1866:1870	wall teichoic acids	1852:1870	wall teichoic acids	1852:1870	In conclusion, there is a novel connection between wall teichoic acids, autolysis, and vancomycin susceptibility in S. aureus.					
34668723	10	9	theme	α-glycosylated	1780:1793	arg1	WTAs					1795:1798	α-glycosylated WTAs	1780:1798	α-glycosylated WTAs	1780:1798	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	6	10	theme	autolysin	1149:1157	arg1	profile					1159:1165	an altered autolysin profile	1138:1165	an altered autolysin profile	1138:1165	Both S. aureus VC40 and S. aureus VraS(VC40) displayed reduced autolysis and an altered autolysin profile in a zymogram.					
34668723	11	11	theme	wall	1852:1855	arg1	acids					1866:1870	wall teichoic acids	1852:1870	wall teichoic acids	1852:1870	In conclusion, there is a novel connection between wall teichoic acids, autolysis, and vancomycin susceptibility in S. aureus.					
34668723	4	12	dep	vraR	769:772	arg1	4 μg/ml					780:786	4 μg/ml	780:786	4 μg/ml	780:786	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	4	12	dep	vraR	769:772	arg1	MIC					775:777	MIC	775:777	MIC	775:777	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	9	13	theme	β-GlcNAc	1558:1565	arg1	anomers					1567:1573	β-GlcNAc anomers	1558:1573	β-GlcNAc anomers	1558:1573	Salt stress induced the incorporation of β-GlcNAc anomers and drastically increased the vancomycin MIC for S. aureus VC40R.					
34668723	13	14	theme	cell	2103:2106	arg1	lipid					2128:2132	the cell wall building block lipid II	2099:2135	the cell wall building block lipid II	2099:2135	This antibiotic inhibits cell wall biosynthesis by binding to the cell wall building block lipid II.					
34668723	7	15	theme	acids	1308:1312	arg1	substitution					1274:1285	substitution	1274:1285	substitution of the wall teichoic acids (WTAs) in S. aureus VC40	1274:1337	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	7	15	theme	acids	1308:1312	arg1	increase					1216:1223	the significant increase	1200:1223	the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc)	1200:1272	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	11	16	from	connection	1833:1842	arg1	aureus					1920:1925	S. aureus	1917:1925	S. aureus	1917:1925	In conclusion, there is a novel connection between wall teichoic acids, autolysis, and vancomycin susceptibility in S. aureus.					
34668723	14	17	theme	laboratory-generated	2232:2251	arg1	strain					2253:2258	a laboratory-generated strain	2230:2258	a laboratory-generated strain	2230:2258	We set out to characterize the mechanisms leading to decreased vancomycin susceptibility in a laboratory-generated strain, S. aureus VC40.					
34668723	14	17	theme	laboratory-generated	2232:2251	arg1	VC40					2271:2274	S. aureus VC40	2261:2274	S. aureus VC40	2261:2274	We set out to characterize the mechanisms leading to decreased vancomycin susceptibility in a laboratory-generated strain, S. aureus VC40.					
34668723	10	18	theme	β-glycosylated	1654:1667	arg1	WTAs					1669:1672	β-glycosylated WTAs	1654:1672	β-glycosylated WTAs	1654:1672	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	1	19	theme	wide	208:211	arg1	range					213:217	a wide range	206:217	a wide range of infections	206:231	Staphylococcus aureus is an opportunistic pathogen that causes a wide range of infections.					
34668723	16	20	theme	resistance	2467:2476	arg1	mechanism					2478:2486	this resistance mechanism	2462:2486	this resistance mechanism	2462:2486	The low cross-linking, necessary for this resistance mechanism, decreases the stability of the cell wall against lytic enzymes, which separate the daughter cells.					
34668723	4	21	theme	vraS	831:834	arg1	mutations					836:844	the 2 vraS mutations	825:844	the 2 vraS mutations	825:844	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	7	22	theme	wall	1294:1297	arg1	acids					1308:1312	the wall teichoic acids	1290:1312	the wall teichoic acids (WTAs)	1290:1319	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	7	22	theme	wall	1294:1297	arg1	WTAs					1315:1318	WTAs	1315:1318	WTAs	1315:1318	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	8	23	dep	strains	1464:1470	arg1	contrast					1442:1449	contrast	1442:1449	contrast	1442:1449	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	18	24	theme	VC40	3004:3007	arg1	resistance					2980:2989	the resistance	2976:2989	the resistance of S. aureus VC40	2976:3007	By experimentally increasing the proportion of β-N-acetyl-d-glucosamine in a closely related isolate through the induction of salt stress, we could show that the β-conformation of the sugars plays a vital role in the resistance of S. aureus VC40.					
34668723	11	25	theme	vancomycin	1888:1897	arg1	susceptibility					1899:1912	vancomycin susceptibility	1888:1912	vancomycin susceptibility	1888:1912	In conclusion, there is a novel connection between wall teichoic acids, autolysis, and vancomycin susceptibility in S. aureus.					
34668723	5	26	theme	aureus	983:988	arg1	VC40					990:993	S. aureus VC40	980:993	S. aureus VC40	980:993	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	10	27	theme	binding	1688:1694	arg1	affinity					1696:1703	the binding affinity	1684:1703	the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall	1684:1763	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	15	28	theme	low	2355:2357	arg1	cross-linking					2359:2371	low cross-linking	2355:2371	low cross-linking	2355:2371	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	18	29	theme	S.	2994:2995	arg1	VC40					3004:3007	S. aureus VC40	2994:3007	S. aureus VC40	2994:3007	By experimentally increasing the proportion of β-N-acetyl-d-glucosamine in a closely related isolate through the induction of salt stress, we could show that the β-conformation of the sugars plays a vital role in the resistance of S. aureus VC40.					
34668723	9	30	theme	vancomycin	1605:1614	arg1	MIC					1616:1618	the vancomycin MIC	1601:1618	the vancomycin MIC for S. aureus VC40R	1601:1638	Salt stress induced the incorporation of β-GlcNAc anomers and drastically increased the vancomycin MIC for S. aureus VC40R.					
34668723	6	31	theme	reduced	1116:1122	arg1	autolysis					1124:1132	reduced autolysis	1116:1132	reduced autolysis	1116:1132	Both S. aureus VC40 and S. aureus VraS(VC40) displayed reduced autolysis and an altered autolysin profile in a zymogram.					
34668723	8	32	theme	magnetic	1348:1355	arg1	resonance					1357:1365	Nuclear magnetic resonance	1340:1365	Nuclear magnetic resonance (NMR) analysis	1340:1380	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	8	32	theme	magnetic	1348:1355	arg1	NMR					1368:1370	NMR	1368:1370	NMR	1368:1370	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	4	33	theme	revertant	706:714	arg1	VC40R					726:730	a revertant S. aureus VC40R	704:730	a revertant S. aureus VC40R	704:730	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	4	34	dep	background	884:893	arg1	4 μg/ml					901:907	4 μg/ml	901:907	4 μg/ml	901:907	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	4	34	dep	background	884:893	arg1	MIC					896:898	MIC	896:898	MIC	896:898	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	2	35	theme	antibiotic	264:273	arg1	resistance					275:284	antibiotic resistance	264:284	antibiotic resistance that leads to treatment failure	264:316	Due to the rapid evolution of antibiotic resistance that leads to treatment failure, it is important to understand the underlying mechanisms.					
34668723	15	36	theme	thick	2334:2338	arg1	wall					2345:2348	a thick cell wall	2332:2348	a thick cell wall	2332:2348	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	10	37	gly	α-glycosylated	1780:1793	arg1	WTAs					1795:1798	α-glycosylated WTAs	1780:1798	α-glycosylated WTAs	1780:1798	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	9	38	theme	aureus	1627:1632	arg1	VC40R					1634:1638	S. aureus VC40R	1624:1638	S. aureus VC40R	1624:1638	Salt stress induced the incorporation of β-GlcNAc anomers and drastically increased the vancomycin MIC for S. aureus VC40R.					
34668723	15	39	theme	cell	2304:2307	arg1	architecture					2314:2325	an altered cell wall architecture	2293:2325	an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin	2293:2422	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	14	40	theme	decreased	2191:2199	arg1	susceptibility					2212:2225	decreased vancomycin susceptibility	2191:2225	decreased vancomycin susceptibility	2191:2225	We set out to characterize the mechanisms leading to decreased vancomycin susceptibility in a laboratory-generated strain, S. aureus VC40.					
34668723	15	41	theme	wall	2309:2312	arg1	architecture					2314:2325	an altered cell wall architecture	2293:2325	an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin	2293:2422	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	18	42	theme	vital	2962:2966	arg1	role					2968:2971	a vital role	2960:2971	a vital role	2960:2971	By experimentally increasing the proportion of β-N-acetyl-d-glucosamine in a closely related isolate through the induction of salt stress, we could show that the β-conformation of the sugars plays a vital role in the resistance of S. aureus VC40.					
34668723	16	43	theme	low	2429:2431	arg1	cross-linking					2433:2445	The low cross-linking	2425:2445	The low cross-linking	2425:2445	The low cross-linking, necessary for this resistance mechanism, decreases the stability of the cell wall against lytic enzymes, which separate the daughter cells.					
34668723	4	44	theme	VISA	503:506	arg1	strains					508:514	the VISA strains	499:514	the VISA strains	499:514	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	4	45	from	exchanges	598:606	arg1	VraS					632:635	the histidine-kinase VraS	611:635	the histidine-kinase VraS	611:635	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	15	46	theme	altered	2296:2302	arg1	architecture					2314:2325	an altered cell wall architecture	2293:2325	an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin	2293:2422	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	15	47	with	architecture	2314:2325	arg1	wall					2345:2348	a thick cell wall	2332:2348	a thick cell wall	2332:2348	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	0	48	theme	Susceptibility	78:91	arg1	Reduction					54:62	the Reduction	50:62	the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus	50:140	The Role of β-Glycosylated Wall Teichoic Acids in the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus.					
34668723	15	49	theme	binding	2395:2401	arg1	sites					2403:2407	decoy binding sites	2389:2407	decoy binding sites for vancomycin	2389:2422	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	4	50	dep	S.	716:717	arg1	aureus					719:724	aureus	719:724	aureus	719:724	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	4	51	theme	susceptible	872:882	arg1	background					884:893	a susceptible background	870:893	a susceptible background (MIC, 4 μg/ml)	870:908	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	4	52	theme	additional	746:755	arg1	mutation					757:764	an additional mutation	743:764	an additional mutation	743:764	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	3	53	theme	aureus	456:461	arg1	strains					470:476	several laboratory vancomycin-intermediate S. aureus (VISA) strains	410:476	several laboratory vancomycin-intermediate S. aureus (VISA) strains	410:476	Here, the cell wall structures of several laboratory vancomycin-intermediate S. aureus (VISA) strains were analyzed.					
34668723	17	54	theme	cell	2644:2647	arg1	acids					2676:2680	the teichoic acids	2663:2680	the teichoic acids	2663:2680	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	17	54	theme	cell	2644:2647	arg1	polymer					2654:2660	another cell wall polymer	2636:2660	another cell wall polymer	2636:2660	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	3	55	theme	cell	386:389	arg1	structures					396:405	the cell wall structures	382:405	the cell wall structures of several laboratory vancomycin-intermediate S. aureus (VISA) strains	382:476	Here, the cell wall structures of several laboratory vancomycin-intermediate S. aureus (VISA) strains were analyzed.					
34668723	18	56	theme	salt	2889:2892	arg1	stress					2894:2899	salt stress	2889:2899	salt stress	2889:2899	By experimentally increasing the proportion of β-N-acetyl-d-glucosamine in a closely related isolate through the induction of salt stress, we could show that the β-conformation of the sugars plays a vital role in the resistance of S. aureus VC40.					
34668723	3	57	theme	vancomycin-intermediate	429:451	arg1	strains					470:476	several laboratory vancomycin-intermediate S. aureus (VISA) strains	410:476	several laboratory vancomycin-intermediate S. aureus (VISA) strains	410:476	Here, the cell wall structures of several laboratory vancomycin-intermediate S. aureus (VISA) strains were analyzed.					
34668723	10	58	gly	β-glycosylated	1654:1667	arg1	WTAs					1669:1672	β-glycosylated WTAs	1654:1672	β-glycosylated WTAs	1654:1672	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	8	59	theme	β-glycosylated	1419:1432	arg1	WTAs					1434:1437	mostly β-glycosylated WTAs	1412:1437	mostly β-glycosylated WTAs	1412:1437	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	3	60	theme	strains	470:476	arg1	structures					396:405	the cell wall structures	382:405	the cell wall structures of several laboratory vancomycin-intermediate S. aureus (VISA) strains	382:476	Here, the cell wall structures of several laboratory vancomycin-intermediate S. aureus (VISA) strains were analyzed.					
34668723	13	61	theme	building	2113:2120	arg1	lipid					2128:2132	the cell wall building block lipid II	2099:2135	the cell wall building block lipid II	2099:2135	This antibiotic inhibits cell wall biosynthesis by binding to the cell wall building block lipid II.					
34668723	3	62	theme	several	410:416	arg1	strains					470:476	several laboratory vancomycin-intermediate S. aureus (VISA) strains	410:476	several laboratory vancomycin-intermediate S. aureus (VISA) strains	410:476	Here, the cell wall structures of several laboratory vancomycin-intermediate S. aureus (VISA) strains were analyzed.					
34668723	0	63	theme	Wall	27:30	arg1	Acids					41:45	β-Glycosylated Wall Teichoic Acids	12:45	β-Glycosylated Wall Teichoic Acids	12:45	The Role of β-Glycosylated Wall Teichoic Acids in the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus.					
34668723	17	64	theme	wall	2649:2652	arg1	acids					2676:2680	the teichoic acids	2663:2680	the teichoic acids	2663:2680	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	17	64	theme	wall	2649:2652	arg1	polymer					2654:2660	another cell wall polymer	2636:2660	another cell wall polymer	2636:2660	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	5	65	theme	liquid	930:935	arg1	UPLC					953:956	UPLC	953:956	UPLC	953:956	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	5	65	theme	liquid	930:935	arg1	chromatography					937:950	A ultraperformance liquid chromatography	911:950	A ultraperformance liquid chromatography (UPLC) analysis	911:966	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	10	66	theme	major	1718:1722	arg1	AtlA					1708:1711	AtlA	1708:1711	AtlA	1708:1711	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	10	66	theme	major	1718:1722	arg1	autolysin					1724:1732	the major autolysin	1714:1732	the major autolysin of S. aureus	1714:1745	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	17	67	theme	high	2710:2713	arg1	substitution					2715:2726	an unusually high substitution	2697:2726	an unusually high substitution with sugars in the β-conformation	2697:2760	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	0	68	theme	Acids	41:45	arg1	Role					4:7	The Role	0:7	The Role of β-Glycosylated Wall Teichoic Acids in the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus.	0:141	The Role of β-Glycosylated Wall Teichoic Acids in the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus.					
34668723	16	69	theme	cell	2520:2523	arg1	wall					2525:2528	the cell wall	2516:2528	the cell wall	2516:2528	The low cross-linking, necessary for this resistance mechanism, decreases the stability of the cell wall against lytic enzymes, which separate the daughter cells.					
34668723	8	70	theme	other	1458:1462	arg1	strains					1464:1470	the other strains	1454:1470	the other strains	1454:1470	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	7	71	dep	increase	1216:1223	arg1	striking					1187:1194	striking	1187:1194	striking	1187:1194	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	15	72	contain	has	2289:2291	arg2	architecture					2314:2325	an altered cell wall architecture	2293:2325	an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin	2293:2422	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	15	72	contain	has	2289:2291	arg1	strain					2282:2287	This strain	2277:2287	This strain	2277:2287	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	10	73	theme	aureus	1740:1745	arg1	AtlA					1708:1711	AtlA	1708:1711	AtlA	1708:1711	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	10	73	theme	aureus	1740:1745	arg1	autolysin					1724:1732	the major autolysin	1714:1732	the major autolysin of S. aureus	1714:1745	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	13	74	theme	wall	2067:2070	arg1	biosynthesis					2072:2083	cell wall biosynthesis	2062:2083	cell wall biosynthesis	2062:2083	This antibiotic inhibits cell wall biosynthesis by binding to the cell wall building block lipid II.					
34668723	10	75	theme	cell	1755:1758	arg1	wall					1760:1763	the cell wall	1751:1763	the cell wall	1751:1763	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	2	76	theme	treatment	300:308	arg1	failure					310:316	treatment failure	300:316	treatment failure	300:316	Due to the rapid evolution of antibiotic resistance that leads to treatment failure, it is important to understand the underlying mechanisms.					
34668723	6	77	theme	aureus	1069:1074	arg1	VC40					1100:1103	VC40	1100:1103	VC40	1100:1103	Both S. aureus VC40 and S. aureus VraS(VC40) displayed reduced autolysis and an altered autolysin profile in a zymogram.					
34668723	6	77	theme	aureus	1069:1074	arg1	VraS					1095:1098	Both S. aureus VC40 and S. aureus VraS	1061:1098	Both S. aureus VC40 and S. aureus VraS(VC40)	1061:1104	Both S. aureus VC40 and S. aureus VraS(VC40) displayed reduced autolysis and an altered autolysin profile in a zymogram.					
34668723	7	78	theme	S.	1324:1325	arg1	VC40					1334:1337	S. aureus VC40	1324:1337	S. aureus VC40	1324:1337	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	8	79	contain	had	1408:1410	arg1	strain					1401:1406	this strain	1396:1406	this strain	1396:1406	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	8	79	contain	had	1408:1410	arg2	WTAs					1434:1437	mostly β-glycosylated WTAs	1412:1437	mostly β-glycosylated WTAs	1412:1437	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	14	80	theme	S.	2261:2262	arg1	strain					2253:2258	a laboratory-generated strain	2230:2258	a laboratory-generated strain	2230:2258	We set out to characterize the mechanisms leading to decreased vancomycin susceptibility in a laboratory-generated strain, S. aureus VC40.					
34668723	14	80	theme	S.	2261:2262	arg1	VC40					2271:2274	S. aureus VC40	2261:2274	S. aureus VC40	2261:2274	We set out to characterize the mechanisms leading to decreased vancomycin susceptibility in a laboratory-generated strain, S. aureus VC40.					
34668723	7	81	theme	aureus	1327:1332	arg1	VC40					1334:1337	S. aureus VC40	1324:1337	S. aureus VC40	1324:1337	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	16	82	theme	necessary	2448:2456	arg1	cross-linking					2433:2445	The low cross-linking	2425:2445	The low cross-linking	2425:2445	The low cross-linking, necessary for this resistance mechanism, decreases the stability of the cell wall against lytic enzymes, which separate the daughter cells.					
34668723	4	83	theme	full	652:655	arg1	resistance					657:666	full resistance	652:666	full resistance against vancomycin (MIC, 64 μg/ml)	652:701	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	6	84	theme	altered	1141:1147	arg1	profile					1159:1165	an altered autolysin profile	1138:1165	an altered autolysin profile	1138:1165	Both S. aureus VC40 and S. aureus VraS(VC40) displayed reduced autolysis and an altered autolysin profile in a zymogram.					
34668723	9	85	theme	anomers	1567:1573	arg1	incorporation					1541:1553	the incorporation	1537:1553	the incorporation of β-GlcNAc anomers	1537:1573	Salt stress induced the incorporation of β-GlcNAc anomers and drastically increased the vancomycin MIC for S. aureus VC40R.					
34668723	7	86	theme	teichoic	1299:1306	arg1	acids					1308:1312	the wall teichoic acids	1290:1312	the wall teichoic acids (WTAs)	1290:1319	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	7	86	theme	teichoic	1299:1306	arg1	WTAs					1315:1318	WTAs	1315:1318	WTAs	1315:1318	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	5	87	theme	S.	980:981	arg1	VC40					990:993	S. aureus VC40	980:993	S. aureus VC40	980:993	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	2	88	theme	rapid	245:249	arg1	evolution					251:259	the rapid evolution	241:259	the rapid evolution of antibiotic resistance that leads to treatment failure	241:316	Due to the rapid evolution of antibiotic resistance that leads to treatment failure, it is important to understand the underlying mechanisms.					
34668723	1	89	theme	infections	222:231	arg1	range					213:217	a wide range	206:217	a wide range of infections	206:231	Staphylococcus aureus is an opportunistic pathogen that causes a wide range of infections.					
34668723	0	90	from	Reduction	54:62	arg1	Vancomycin-Intermediate					96:118	Vancomycin-Intermediate	96:118	Vancomycin-Intermediate Staphylococcus aureus	96:140	The Role of β-Glycosylated Wall Teichoic Acids in the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus.					
34668723	11	91	theme	novel	1827:1831	arg1	connection					1833:1842	a novel connection	1825:1842	a novel connection between wall teichoic acids, autolysis, and vancomycin susceptibility in S. aureus	1825:1925	In conclusion, there is a novel connection between wall teichoic acids, autolysis, and vancomycin susceptibility in S. aureus.					
34668723	17	92	with	substitution	2715:2726	arg1	sugars					2733:2738	sugars	2733:2738	sugars in the β-conformation	2733:2760	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	18	93	theme	sugars	2947:2952	arg1	β-conformation					2925:2938	the β-conformation	2921:2938	the β-conformation of the sugars	2921:2952	By experimentally increasing the proportion of β-N-acetyl-d-glucosamine in a closely related isolate through the induction of salt stress, we could show that the β-conformation of the sugars plays a vital role in the resistance of S. aureus VC40.					
34668723	5	94	theme	peptidoglycan	1046:1058	arg1	cross-linking					1025:1037	a significantly decreased cross-linking	999:1037	a significantly decreased cross-linking of the peptidoglycan	999:1058	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	8	95	theme	resonance	1357:1365	arg1	analysis					1373:1380	Nuclear magnetic resonance (NMR) analysis	1340:1380	Nuclear magnetic resonance (NMR) analysis	1340:1380	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	4	96	contain	has	739:741	arg1	VC40R					726:730	a revertant S. aureus VC40R	704:730	a revertant S. aureus VC40R	704:730	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	4	96	contain	has	739:741	arg2	mutation					757:764	an additional mutation	743:764	an additional mutation	743:764	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	18	97	theme	β-N-acetyl-d-glucosamine	2810:2833	arg1	proportion					2796:2805	the proportion	2792:2805	the proportion of β-N-acetyl-d-glucosamine in a closely related isolate	2792:2862	By experimentally increasing the proportion of β-N-acetyl-d-glucosamine in a closely related isolate through the induction of salt stress, we could show that the β-conformation of the sugars plays a vital role in the resistance of S. aureus VC40.					
34668723	2	98	theme	resistance	275:284	arg1	evolution					251:259	the rapid evolution	241:259	the rapid evolution of antibiotic resistance that leads to treatment failure	241:316	Due to the rapid evolution of antibiotic resistance that leads to treatment failure, it is important to understand the underlying mechanisms.					
34668723	9	99	theme	S.	1624:1625	arg1	VC40R					1634:1638	S. aureus VC40R	1624:1638	S. aureus VC40R	1624:1638	Salt stress induced the incorporation of β-GlcNAc anomers and drastically increased the vancomycin MIC for S. aureus VC40R.					
34668723	12	100	theme	IMPORTANCE	1928:1937	arg1	Infections					1939:1948	IMPORTANCE Infections	1928:1948	IMPORTANCE Infections with methicillin-resistant Staphylococcus aureus	1928:1997	IMPORTANCE Infections with methicillin-resistant Staphylococcus aureus are commonly treated with vancomycin.					
34668723	14	101	theme	vancomycin	2201:2210	arg1	susceptibility					2212:2225	decreased vancomycin susceptibility	2191:2225	decreased vancomycin susceptibility	2191:2225	We set out to characterize the mechanisms leading to decreased vancomycin susceptibility in a laboratory-generated strain, S. aureus VC40.					
34668723	10	102	theme	AtlA	1708:1711	arg1	affinity					1696:1703	the binding affinity	1684:1703	the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall	1684:1763	In addition, β-glycosylated WTAs decreased the binding affinity of AtlA, the major autolysin of S. aureus, to the cell wall, compared with α-glycosylated WTAs.					
34668723	15	103	theme	cell	2340:2343	arg1	wall					2345:2348	a thick cell wall	2332:2348	a thick cell wall	2332:2348	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	17	104	theme	teichoic	2667:2674	arg1	acids					2676:2680	the teichoic acids	2663:2680	the teichoic acids	2663:2680	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	17	104	theme	teichoic	2667:2674	arg1	polymer					2654:2660	another cell wall polymer	2636:2660	another cell wall polymer	2636:2660	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	17	105	contain	contain	2689:2695	arg1	acids					2676:2680	the teichoic acids	2663:2680	the teichoic acids	2663:2680	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	17	105	contain	contain	2689:2695	arg1	polymer					2654:2660	another cell wall polymer	2636:2660	another cell wall polymer	2636:2660	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	17	105	contain	contain	2689:2695	arg2	substitution					2715:2726	an unusually high substitution	2697:2726	an unusually high substitution with sugars in the β-conformation	2697:2760	Protection against these enzymes is provided by another cell wall polymer, the teichoic acids, which contain an unusually high substitution with sugars in the β-conformation.					
34668723	0	106	theme	Vancomycin	67:76	arg1	Susceptibility					78:91	Vancomycin Susceptibility	67:91	Vancomycin Susceptibility	67:91	The Role of β-Glycosylated Wall Teichoic Acids in the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus.					
34668723	1	107	theme	opportunistic	171:183	arg1	aureus					158:163	Staphylococcus aureus	143:163	Staphylococcus aureus	143:163	Staphylococcus aureus is an opportunistic pathogen that causes a wide range of infections.					
34668723	1	107	theme	opportunistic	171:183	arg1	pathogen					185:192	an opportunistic pathogen	168:192	an opportunistic pathogen that causes a wide range of infections	168:231	Staphylococcus aureus is an opportunistic pathogen that causes a wide range of infections.					
34668723	15	108	with	wall	2345:2348	arg1	cross-linking					2359:2371	low cross-linking	2355:2371	low cross-linking	2355:2371	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	0	109	from	Role	4:7	arg1	Reduction					54:62	the Reduction	50:62	the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus	50:140	The Role of β-Glycosylated Wall Teichoic Acids in the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus.					
34668723	4	110	dep	aureus	524:529	arg1	VC40					531:534	VC40	531:534	VC40	531:534	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	8	111	gly	β-glycosylated	1419:1432	arg1	WTAs					1434:1437	mostly β-glycosylated WTAs	1412:1437	mostly β-glycosylated WTAs	1412:1437	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	16	112	theme	daughter	2572:2579	arg1	cells					2581:2585	the daughter cells	2568:2585	the daughter cells	2568:2585	The low cross-linking, necessary for this resistance mechanism, decreases the stability of the cell wall against lytic enzymes, which separate the daughter cells.					
34668723	5	113	contain	had	995:997	arg2	cross-linking					1025:1037	a significantly decreased cross-linking	999:1037	a significantly decreased cross-linking of the peptidoglycan	999:1058	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	5	113	contain	had	995:997	arg1	VC40					990:993	S. aureus VC40	980:993	S. aureus VC40	980:993	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	18	114	theme	related	2848:2854	arg1	isolate					2856:2862	a closely related isolate	2838:2862	a closely related isolate	2838:2862	By experimentally increasing the proportion of β-N-acetyl-d-glucosamine in a closely related isolate through the induction of salt stress, we could show that the β-conformation of the sugars plays a vital role in the resistance of S. aureus VC40.					
34668723	5	115	theme	decreased	1015:1023	arg1	cross-linking					1025:1037	a significantly decreased cross-linking	999:1037	a significantly decreased cross-linking of the peptidoglycan	999:1058	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	15	116	theme	decoy	2389:2393	arg1	sites					2403:2407	decoy binding sites	2389:2407	decoy binding sites for vancomycin	2389:2422	This strain has an altered cell wall architecture with a thick cell wall with low cross-linking, which provides decoy binding sites for vancomycin.					
34668723	7	117	theme	significant	1204:1214	arg1	increase					1216:1223	the significant increase	1200:1223	the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc)	1200:1272	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	7	117	theme	significant	1204:1214	arg1	substitution					1274:1285	substitution	1274:1285	substitution of the wall teichoic acids (WTAs) in S. aureus VC40	1274:1337	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	3	118	theme	wall	391:394	arg1	structures					396:405	the cell wall structures	382:405	the cell wall structures of several laboratory vancomycin-intermediate S. aureus (VISA) strains	382:476	Here, the cell wall structures of several laboratory vancomycin-intermediate S. aureus (VISA) strains were analyzed.					
34668723	4	119	theme	S.	716:717	arg1	VC40R					726:730	a revertant S. aureus VC40R	704:730	a revertant S. aureus VC40R	704:730	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	0	120	theme	β-Glycosylated	12:25	arg1	Acids					41:45	β-Glycosylated Wall Teichoic Acids	12:45	β-Glycosylated Wall Teichoic Acids	12:45	The Role of β-Glycosylated Wall Teichoic Acids in the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus.					
34668723	13	121	theme	wall	2108:2111	arg1	lipid					2128:2132	the cell wall building block lipid II	2099:2135	the cell wall building block lipid II	2099:2135	This antibiotic inhibits cell wall biosynthesis by binding to the cell wall building block lipid II.					
34668723	18	122	theme	stress	2894:2899	arg1	induction					2876:2884	the induction	2872:2884	the induction of salt stress	2872:2899	By experimentally increasing the proportion of β-N-acetyl-d-glucosamine in a closely related isolate through the induction of salt stress, we could show that the β-conformation of the sugars plays a vital role in the resistance of S. aureus VC40.					
34668723	7	123	from	substitution	1274:1285	arg1	VC40					1334:1337	S. aureus VC40	1324:1337	S. aureus VC40	1324:1337	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	0	124	theme	Teichoic	32:39	arg1	Acids					41:45	β-Glycosylated Wall Teichoic Acids	12:45	β-Glycosylated Wall Teichoic Acids	12:45	The Role of β-Glycosylated Wall Teichoic Acids in the Reduction of Vancomycin Susceptibility in Vancomycin-Intermediate Staphylococcus aureus.					
34668723	13	125	theme	block	2122:2126	arg1	lipid					2128:2132	the cell wall building block lipid II	2099:2135	the cell wall building block lipid II	2099:2135	This antibiotic inhibits cell wall biosynthesis by binding to the cell wall building block lipid II.					
34668723	18	126	theme	aureus	2997:3002	arg1	VC40					3004:3007	S. aureus VC40	2994:3007	S. aureus VC40	2994:3007	By experimentally increasing the proportion of β-N-acetyl-d-glucosamine in a closely related isolate through the induction of salt stress, we could show that the β-conformation of the sugars plays a vital role in the resistance of S. aureus VC40.					
34668723	5	127	theme	ultraperformance	913:928	arg1	UPLC					953:956	UPLC	953:956	UPLC	953:956	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	5	127	theme	ultraperformance	913:928	arg1	chromatography					937:950	A ultraperformance liquid chromatography	911:950	A ultraperformance liquid chromatography (UPLC) analysis	911:966	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	3	128	theme	laboratory	418:427	arg1	strains					470:476	several laboratory vancomycin-intermediate S. aureus (VISA) strains	410:476	several laboratory vancomycin-intermediate S. aureus (VISA) strains	410:476	Here, the cell wall structures of several laboratory vancomycin-intermediate S. aureus (VISA) strains were analyzed.					
34668723	5	129	theme	chromatography	937:950	arg1	analysis					959:966	A ultraperformance liquid chromatography (UPLC) analysis	911:966	A ultraperformance liquid chromatography (UPLC) analysis	911:966	A ultraperformance liquid chromatography (UPLC) analysis showed that S. aureus VC40 had a significantly decreased cross-linking of the peptidoglycan.					
34668723	16	130	theme	wall	2525:2528	arg1	stability					2503:2511	the stability	2499:2511	the stability of the cell wall against lytic enzymes, which separate the daughter cells	2499:2585	The low cross-linking, necessary for this resistance mechanism, decreases the stability of the cell wall against lytic enzymes, which separate the daughter cells.					
34668723	8	131	theme	Nuclear	1340:1346	arg1	resonance					1357:1365	Nuclear magnetic resonance	1340:1365	Nuclear magnetic resonance (NMR) analysis	1340:1380	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	8	131	theme	Nuclear	1340:1346	arg1	NMR					1368:1370	NMR	1368:1370	NMR	1368:1370	Nuclear magnetic resonance (NMR) analysis revealed that this strain had mostly β-glycosylated WTAs in contrast to the other strains, which showed only the α-glycosylation peak.					
34668723	4	132	dep	vancomycin	676:685	arg1	64 μg/ml					693:700	64 μg/ml	693:700	64 μg/ml	693:700	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	4	132	dep	vancomycin	676:685	arg1	MIC					688:690	MIC	688:690	MIC	688:690	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
34668723	16	133	theme	lytic	2538:2542	arg1	enzymes					2544:2550	lytic enzymes	2538:2550	lytic enzymes	2538:2550	The low cross-linking, necessary for this resistance mechanism, decreases the stability of the cell wall against lytic enzymes, which separate the daughter cells.					
34668723	12	134	dep	Staphylococcus	1977:1990	arg1	aureus					1992:1997	aureus	1992:1997	aureus	1992:1997	IMPORTANCE Infections with methicillin-resistant Staphylococcus aureus are commonly treated with vancomycin.					
34668723	13	135	theme	cell	2062:2065	arg1	biosynthesis					2072:2083	cell wall biosynthesis	2062:2083	cell wall biosynthesis	2062:2083	This antibiotic inhibits cell wall biosynthesis by binding to the cell wall building block lipid II.					
34668723	7	136	from	increase	1216:1223	arg1	GlcNAc					1266:1271	GlcNAc	1266:1271	GlcNAc	1266:1271	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	7	136	from	increase	1216:1223	arg1	N-acetyl-d-glucosamine					1242:1263	N-acetyl-d-glucosamine	1242:1263	N-acetyl-d-glucosamine (GlcNAc)	1242:1272	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	7	136	from	increase	1216:1223	arg1	d-alanine					1228:1236	d-alanine	1228:1236	d-alanine	1228:1236	Most striking was the significant increase in d-alanine and N-acetyl-d-glucosamine (GlcNAc) substitution of the wall teichoic acids (WTAs) in S. aureus VC40.					
34668723	4	137	theme	histidine-kinase	615:630	arg1	VraS					632:635	the histidine-kinase VraS	611:635	the histidine-kinase VraS	611:635	Among the VISA strains were S. aureus VC40, which accumulated 79 mutations, including most importantly 2 exchanges in the histidine-kinase VraS, and developed full resistance against vancomycin (MIC, 64 μg/ml); a revertant S. aureus VC40R, which has an additional mutation in vraR (MIC, 4 μg/ml); and S. aureus VraS(VC40), in which the 2 vraS mutations were reconstituted into a susceptible background (MIC, 4 μg/ml).					
33889819	6	0	theme	Further	1024:1030	arg1	analysis					1044:1051	Further advanced MS analysis	1024:1051	Further advanced MS analysis	1024:1051	Further advanced MS analysis showed the additional sialic acid is bonded through an α2-6 linkage to the peripheral N-acetylglucosamine residue.					
33889819	0	1	with	patient	26:32	arg1	variant					47:53	a HNF1α variant	39:53	a HNF1α variant	39:53	Aberrant sialylation in a patient with a HNF1α variant and liver adenomatosis.					
33889819	0	1	with	patient	26:32	arg1	adenomatosis					65:76	liver adenomatosis	59:76	liver adenomatosis	59:76	Aberrant sialylation in a patient with a HNF1α variant and liver adenomatosis.					
33889819	4	2	theme	N-glycosylation	754:768	arg1	pattern					770:776	a surprising N-glycosylation pattern	741:776	a surprising N-glycosylation pattern consisting on hyposialylation	741:806	Serum transferrin isoelectric focusing showed a surprising N-glycosylation pattern consisting on hyposialylation, as well as remarkable hypersialylation.					
33889819	6	3	theme	α2-6	1108:1111	arg1	linkage					1113:1119	an α2-6 linkage	1105:1119	an α2-6 linkage to the peripheral N-acetylglucosamine residue	1105:1165	Further advanced MS analysis showed the additional sialic acid is bonded through an α2-6 linkage to the peripheral N-acetylglucosamine residue.					
33889819	0	4	theme	liver	59:63	arg1	adenomatosis					65:76	liver adenomatosis	59:76	liver adenomatosis	59:76	Aberrant sialylation in a patient with a HNF1α variant and liver adenomatosis.					
33889819	0	5	from	sialylation	9:19	arg1	patient					26:32	a patient	24:32	a patient with a HNF1α variant and liver adenomatosis	24:76	Aberrant sialylation in a patient with a HNF1α variant and liver adenomatosis.					
33889819	3	6	gly	N-glycosylation	538:552	arg1	paper					502:506	the present paper	490:506	the present paper	490:506	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	3	6	gly	N-glycosylation	538:552	arg1	patient					573:579	a CDG-candidate patient	557:579	a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant	557:692	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	5	7	theme	sialic	998:1003	arg1	acids					1005:1009	up to two sialic acids	988:1009	up to two sialic acids	988:1009	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	3	8	theme	unique	525:530	arg1	N-glycosylation					538:552	the unique serum N-glycosylation	521:552	the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant	521:692	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	2	9	theme	glycan	309:314	arg1	biosynthesis					316:327	a defective glycan biosynthesis	297:327	a defective glycan biosynthesis	297:327	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
33889819	3	10	theme	CDG-candidate	559:571	arg1	patient					573:579	a CDG-candidate patient	557:579	a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant	557:692	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	2	11	theme	defective	299:307	arg1	biosynthesis					316:327	a defective glycan biosynthesis	297:327	a defective glycan biosynthesis	297:327	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
33889819	5	12	theme	individual	894:903	arg1	glycoproteins					911:923	individual serum glycoproteins	894:923	individual serum glycoproteins	894:923	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	4	13	theme	focusing	725:732	arg1	transferrin					701:711	Serum transferrin	695:711	Serum transferrin isoelectric focusing	695:732	Serum transferrin isoelectric focusing showed a surprising N-glycosylation pattern consisting on hyposialylation, as well as remarkable hypersialylation.					
33889819	1	14	theme	structural	172:181	arg1	diversity					183:191	their structural diversity	166:191	their structural diversity	166:191	Glycosylation is a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions.					
33889819	6	15	theme	N-acetylglucosamine	1139:1157	arg1	residue					1159:1165	the peripheral N-acetylglucosamine residue	1124:1165	the peripheral N-acetylglucosamine residue	1124:1165	Further advanced MS analysis showed the additional sialic acid is bonded through an α2-6 linkage to the peripheral N-acetylglucosamine residue.					
33889819	5	16	theme	serum	905:909	arg1	glycoproteins					911:923	individual serum glycoproteins	894:923	individual serum glycoproteins	894:923	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	2	17	theme	disorders	413:421	arg1	area					394:397	the wide area	385:397	the wide area of congenital disorders of glycosylation (CDG)	385:444	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
33889819	2	18	theme	multisystem	462:472	arg1	involvement					474:484	multisystem involvement	462:484	multisystem involvement	462:484	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
33889819	3	19	with	patient	573:579	arg1	phenotype					614:622	an unexplained neurological phenotype	586:622	an unexplained neurological phenotype	586:622	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	3	19	with	patient	573:579	arg1	adenomatosis					634:645	liver adenomatosis	628:645	liver adenomatosis	628:645	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	2	20	theme	congenital	402:411	arg1	disorders					413:421	congenital disorders	402:421	congenital disorders of glycosylation (CDG)	402:444	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
33889819	0	21	theme	Aberrant	0:7	arg1	sialylation					9:19	Aberrant sialylation	0:19	Aberrant sialylation in a patient with a HNF1α variant and liver adenomatosis.	0:77	Aberrant sialylation in a patient with a HNF1α variant and liver adenomatosis.					
33889819	5	22	gly	hypersialylated	943:957	arg1	N-glycans					967:975	hypersialylated complex N-glycans	943:975	hypersialylated complex N-glycans comprising up to two sialic acids per antenna	943:1021	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	3	23	theme	unexplained	589:599	arg1	phenotype					614:622	an unexplained neurological phenotype	586:622	an unexplained neurological phenotype	586:622	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	1	24	theme	subtle	203:208	arg1	properties					237:246	subtle and specialized biological properties	203:246	subtle and specialized biological properties	203:246	Glycosylation is a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions.					
33889819	5	25	theme	glycoproteins	911:923	arg1	analyses					882:889	Mass spectrometry-based glycomic analyses	849:889	Mass spectrometry-based glycomic analyses of individual serum glycoproteins	849:923	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	3	26	theme	patient	573:579	arg1	N-glycosylation					538:552	the unique serum N-glycosylation	521:552	the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant	521:692	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	6	27	theme	sialic	1075:1080	arg1	acid					1082:1085	the additional sialic acid	1060:1085	the additional sialic acid	1060:1085	Further advanced MS analysis showed the additional sialic acid is bonded through an α2-6 linkage to the peripheral N-acetylglucosamine residue.					
33889819	4	28	theme	remarkable	820:829	arg1	hypersialylation					831:846	remarkable hypersialylation	820:846	remarkable hypersialylation	820:846	Serum transferrin isoelectric focusing showed a surprising N-glycosylation pattern consisting on hyposialylation, as well as remarkable hypersialylation.					
33889819	4	29	theme	surprising	743:752	arg1	pattern					770:776	a surprising N-glycosylation pattern	741:776	a surprising N-glycosylation pattern consisting on hyposialylation	741:806	Serum transferrin isoelectric focusing showed a surprising N-glycosylation pattern consisting on hyposialylation, as well as remarkable hypersialylation.					
33889819	1	30	theme	specialized	214:224	arg1	properties					237:246	subtle and specialized biological properties	203:246	subtle and specialized biological properties	203:246	Glycosylation is a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions.					
33889819	5	31	theme	spectrometry-based	854:871	arg1	analyses					882:889	Mass spectrometry-based glycomic analyses	849:889	Mass spectrometry-based glycomic analyses of individual serum glycoproteins	849:923	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	6	32	theme	additional	1064:1073	arg1	acid					1082:1085	the additional sialic acid	1060:1085	the additional sialic acid	1060:1085	Further advanced MS analysis showed the additional sialic acid is bonded through an α2-6 linkage to the peripheral N-acetylglucosamine residue.					
33889819	5	33	theme	hypersialylated	943:957	arg1	N-glycans					967:975	hypersialylated complex N-glycans	943:975	hypersialylated complex N-glycans comprising up to two sialic acids per antenna	943:1021	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	1	34	theme	biological	226:235	arg1	properties					237:246	subtle and specialized biological properties	203:246	subtle and specialized biological properties	203:246	Glycosylation is a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions.					
33889819	5	35	theme	glycomic	873:880	arg1	analyses					882:889	Mass spectrometry-based glycomic analyses	849:889	Mass spectrometry-based glycomic analyses of individual serum glycoproteins	849:923	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	3	36	theme	pathogenic	669:678	arg1	variant					686:692	a recurrent pathogenic HNF1α variant	657:692	a recurrent pathogenic HNF1α variant	657:692	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	0	37	gly	sialylation	9:19	arg1	patient					26:32	a patient	24:32	a patient with a HNF1α variant and liver adenomatosis	24:76	Aberrant sialylation in a patient with a HNF1α variant and liver adenomatosis.					
33889819	2	38	theme	glycosylation	426:438	arg1	disorders					413:421	congenital disorders	402:421	congenital disorders of glycosylation (CDG)	402:444	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
33889819	0	39	theme	HNF1α	41:45	arg1	variant					47:53	a HNF1α variant	39:53	a HNF1α variant	39:53	Aberrant sialylation in a patient with a HNF1α variant and liver adenomatosis.					
33889819	2	40	gly	glycoproteins	359:371	arg1	glycoproteins					359:371	the nascent glycoproteins	347:371	the nascent glycoproteins	347:371	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
33889819	3	41	theme	HNF1α	680:684	arg1	variant					686:692	a recurrent pathogenic HNF1α variant	657:692	a recurrent pathogenic HNF1α variant	657:692	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	3	42	theme	neurological	601:612	arg1	phenotype					614:622	an unexplained neurological phenotype	586:622	an unexplained neurological phenotype	586:622	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	1	43	theme	fundamental	98:108	arg1	modification					129:140	a fundamental post-translational modification	96:140	a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions	96:260	Glycosylation is a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions.					
33889819	1	43	theme	fundamental	98:108	arg1	Glycosylation					79:91	Glycosylation	79:91	Glycosylation	79:91	Glycosylation is a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions.					
33889819	5	44	gly	glycoproteins	911:923	arg1	glycoproteins					911:923	individual serum glycoproteins	894:923	individual serum glycoproteins	894:923	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	1	45	theme	post-translational	110:127	arg1	modification					129:140	a fundamental post-translational modification	96:140	a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions	96:260	Glycosylation is a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions.					
33889819	1	45	theme	post-translational	110:127	arg1	Glycosylation					79:91	Glycosylation	79:91	Glycosylation	79:91	Glycosylation is a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions.					
33889819	6	46	theme	MS	1041:1042	arg1	analysis					1044:1051	Further advanced MS analysis	1024:1051	Further advanced MS analysis	1024:1051	Further advanced MS analysis showed the additional sialic acid is bonded through an α2-6 linkage to the peripheral N-acetylglucosamine residue.					
33889819	4	47	dep	focusing	725:732	arg1	isoelectric					713:723	isoelectric	713:723	isoelectric	713:723	Serum transferrin isoelectric focusing showed a surprising N-glycosylation pattern consisting on hyposialylation, as well as remarkable hypersialylation.					
33889819	2	48	theme	due	290:292	arg1	diseases					281:288	All those genetic diseases	263:288	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins	263:371	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
33889819	3	49	theme	liver	628:632	arg1	adenomatosis					634:645	liver adenomatosis	628:645	liver adenomatosis	628:645	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	2	50	theme	nascent	351:357	arg1	glycoproteins					359:371	the nascent glycoproteins	347:371	the nascent glycoproteins	347:371	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
33889819	5	51	dep	two	994:996	arg1	to					991:992	to	991:992	to	991:992	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	6	52	theme	peripheral	1128:1137	arg1	residue					1159:1165	the peripheral N-acetylglucosamine residue	1124:1165	the peripheral N-acetylglucosamine residue	1124:1165	Further advanced MS analysis showed the additional sialic acid is bonded through an α2-6 linkage to the peripheral N-acetylglucosamine residue.					
33889819	3	53	theme	recurrent	659:667	arg1	variant					686:692	a recurrent pathogenic HNF1α variant	657:692	a recurrent pathogenic HNF1α variant	657:692	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	3	54	theme	serum	532:536	arg1	N-glycosylation					538:552	the unique serum N-glycosylation	521:552	the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant	521:692	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	3	55	theme	present	494:500	arg1	paper					502:506	the present paper	490:506	the present paper	490:506	In the present paper, we detailed the unique serum N-glycosylation of a CDG-candidate patient with an unexplained neurological phenotype and liver adenomatosis harboring a recurrent pathogenic HNF1α variant.					
33889819	5	56	theme	complex	959:965	arg1	N-glycans					967:975	hypersialylated complex N-glycans	943:975	hypersialylated complex N-glycans comprising up to two sialic acids per antenna	943:1021	Mass spectrometry-based glycomic analyses of individual serum glycoproteins enabled to unveil hypersialylated complex N-glycans comprising up to two sialic acids per antenna.					
33889819	2	57	theme	genetic	273:279	arg1	diseases					281:288	All those genetic diseases	263:288	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins	263:371	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
33889819	1	58	theme	proteins	145:152	arg1	modification					129:140	a fundamental post-translational modification	96:140	a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions	96:260	Glycosylation is a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions.					
33889819	1	58	theme	proteins	145:152	arg1	Glycosylation					79:91	Glycosylation	79:91	Glycosylation	79:91	Glycosylation is a fundamental post-translational modification of proteins that boosts their structural diversity providing subtle and specialized biological properties and functions.					
33889819	4	59	theme	Serum	695:699	arg1	transferrin					701:711	Serum transferrin	695:711	Serum transferrin isoelectric focusing	695:732	Serum transferrin isoelectric focusing showed a surprising N-glycosylation pattern consisting on hyposialylation, as well as remarkable hypersialylation.					
33889819	6	60	theme	advanced	1032:1039	arg1	analysis					1044:1051	Further advanced MS analysis	1024:1051	Further advanced MS analysis	1024:1051	Further advanced MS analysis showed the additional sialic acid is bonded through an α2-6 linkage to the peripheral N-acetylglucosamine residue.					
33889819	2	61	theme	wide	389:392	arg1	area					394:397	the wide area	385:397	the wide area of congenital disorders of glycosylation (CDG)	385:444	All those genetic diseases due to a defective glycan biosynthesis and attachment to the nascent glycoproteins fall within the wide area of congenital disorders of glycosylation (CDG), mostly causing multisystem involvement.					
34890647	8	0	theme	binding	1567:1573	arg1	contacts					1575:1582	glycan-protein binding contacts	1552:1582	glycan-protein binding contacts within the IgG1-FcγRI complex	1552:1612	Moreover, HDX-MS data evince that the Fab domain has glycan-protein binding contacts within the IgG1-FcγRI complex.					
34890647	3	1	theme	molecular	843:851	arg1	MD					863:864	MD	863:864	MD	863:864	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	3	1	theme	molecular	843:851	arg1	dynamics					853:860	molecular dynamics	843:860	molecular dynamics (MD) simulations	843:877	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	0	2	theme	IgG1-FcγRIa	68:78	arg1	Complex					95:101	the IgG1-FcγRIa (CD64a) Immune Complex	64:101	the IgG1-FcγRIa (CD64a) Immune Complex	64:101	HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds.					
34890647	8	3	theme	glycan-protein	1552:1565	arg1	contacts					1575:1582	glycan-protein binding contacts	1552:1582	glycan-protein binding contacts within the IgG1-FcγRI complex	1552:1612	Moreover, HDX-MS data evince that the Fab domain has glycan-protein binding contacts within the IgG1-FcγRI complex.					
34890647	5	4	theme	percent	1103:1109	arg1	calculation					1084:1094	calculation	1084:1094	calculation of the percent of time	1084:1117	MD simulations corroborate the differences in the peptide deuterium content through calculation of the percent of time that transient glycan-peptide bonds exist.					
34890647	4	5	theme	distinct	971:978	arg1	rates					993:997	distinct H/D exchange rates	971:997	distinct H/D exchange rates	971:997	For each glycoform of the IgG1-FcγRIa complex peptic peptides of Fab, Fc and FcγRIa report distinct H/D exchange rates.					
34890647	2	6	gly	glycoprotein	410:421	arg1	glycoprotein					410:421	glycoprotein contacts	410:430	glycoprotein contacts between glycans of IgG1	410:454	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	4	7	theme	H/D	980:982	arg1	rates					993:997	distinct H/D exchange rates	971:997	distinct H/D exchange rates	971:997	For each glycoform of the IgG1-FcγRIa complex peptic peptides of Fab, Fc and FcγRIa report distinct H/D exchange rates.					
34890647	0	8	theme	CD64a	81:85	arg1	Complex					95:101	the IgG1-FcγRIa (CD64a) Immune Complex	64:101	the IgG1-FcγRIa (CD64a) Immune Complex	64:101	HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds.					
34890647	5	9	theme	transient	1124:1132	arg1	bonds					1149:1153	transient glycan-peptide bonds	1124:1153	transient glycan-peptide bonds	1124:1153	MD simulations corroborate the differences in the peptide deuterium content through calculation of the percent of time that transient glycan-peptide bonds exist.					
34890647	4	10	theme	IgG1-FcγRIa	906:916	arg1	complex					918:924	the IgG1-FcγRIa complex	902:924	the IgG1-FcγRIa complex	902:924	For each glycoform of the IgG1-FcγRIa complex peptic peptides of Fab, Fc and FcγRIa report distinct H/D exchange rates.					
34890647	2	11	theme	L235	286:289	arg1	importance					272:281	the importance	268:281	the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization	268:353	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	5	12	from	differences	1031:1041	arg1	content					1068:1074	the peptide deuterium content	1046:1074	the peptide deuterium content	1046:1074	MD simulations corroborate the differences in the peptide deuterium content through calculation of the percent of time that transient glycan-peptide bonds exist.					
34890647	7	13	theme	complexed	1483:1491	arg1	IgG1					1493:1496	isolated and complexed IgG1	1470:1496	isolated and complexed IgG1	1470:1496	The results also indicate that intramolecular glycan-protein bonds stabilize the Fc region in isolated and complexed IgG1.					
34890647	0	14	theme	Complex	95:101	arg1	Stabilization					47:59	Stabilization	47:59	Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds	47:143	HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds.					
34890647	5	15	theme	glycan-peptide	1134:1147	arg1	bonds					1149:1153	transient glycan-peptide bonds	1124:1153	transient glycan-peptide bonds	1124:1153	MD simulations corroborate the differences in the peptide deuterium content through calculation of the percent of time that transient glycan-peptide bonds exist.					
34890647	6	16	theme	173KHR175	1330:1338	arg1	motif					1340:1344	the 173KHR175 motif	1326:1344	the 173KHR175 motif within the FG-loop of FcγRIa	1326:1373	These results indicate that stability of IgG1-FcγRIa complexes correlate with the presence of intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa.					
34890647	3	17	theme	FcγRIa	532:537	arg1	receptor					539:546	the FcγRIa receptor	528:546	the FcγRIa receptor	528:546	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	1	18	theme	different	171:179	arg1	models					181:186	different models	171:186	different models for the stabilization of the Fc-FcγRI immune complex	171:239	Previous reports present different models for the stabilization of the Fc-FcγRI immune complex.					
34890647	0	19	theme	Immune	88:93	arg1	Complex					95:101	the IgG1-FcγRIa (CD64a) Immune Complex	64:101	the IgG1-FcγRIa (CD64a) Immune Complex	64:101	HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds.					
34890647	3	20	theme	dynamics	853:860	arg1	simulations					867:877	molecular dynamics (MD) simulations	843:877	molecular dynamics (MD) simulations	843:877	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	4	21	theme	exchange	984:991	arg1	rates					993:997	distinct H/D exchange rates	971:997	distinct H/D exchange rates	971:997	For each glycoform of the IgG1-FcγRIa complex peptic peptides of Fab, Fc and FcγRIa report distinct H/D exchange rates.					
34890647	6	22	theme	IgG1-FcγRIa	1203:1213	arg1	complexes					1215:1223	IgG1-FcγRIa complexes	1203:1223	IgG1-FcγRIa complexes	1203:1223	These results indicate that stability of IgG1-FcγRIa complexes correlate with the presence of intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa.					
34890647	2	23	theme	hydrophobic	308:318	arg1	contacts					320:327	some hydrophobic contacts	303:327	some hydrophobic contacts for complex stabilization	303:353	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	4	24	theme	peptic	926:931	arg1	peptides					933:940	peptic peptides	926:940	peptic peptides of Fab, Fc and FcγRIa	926:962	For each glycoform of the IgG1-FcγRIa complex peptic peptides of Fab, Fc and FcγRIa report distinct H/D exchange rates.					
34890647	2	25	theme	IgG1	451:454	arg1	glycans					440:446	glycans	440:446	glycans of IgG1	440:454	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	3	26	contain	containing	558:567	arg2	glycans					581:587	biantennary glycans	569:587	biantennary glycans	569:587	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	3	26	contain	containing	558:567	arg1	Complexes					506:514	Complexes	506:514	Complexes formed from the FcγRIa receptor	506:546	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	0	27	theme	Intermolecular	111:124	arg1	Bonds					139:143	Intermolecular Glycoprotein Bonds	111:143	Intermolecular Glycoprotein Bonds	111:143	HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds.					
34890647	7	28	theme	Fc	1457:1458	arg1	region					1460:1465	the Fc region	1453:1465	the Fc region in isolated and complexed IgG1	1453:1496	The results also indicate that intramolecular glycan-protein bonds stabilize the Fc region in isolated and complexed IgG1.					
34890647	7	29	theme	isolated	1470:1477	arg1	IgG1					1493:1496	isolated and complexed IgG1	1470:1496	isolated and complexed IgG1	1470:1496	The results also indicate that intramolecular glycan-protein bonds stabilize the Fc region in isolated and complexed IgG1.					
34890647	0	30	theme	MD	11:12	arg1	Simulations					14:24	MD Simulations	11:24	MD Simulations	11:24	HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds.					
34890647	8	31	theme	IgG1-FcγRI	1595:1604	arg1	complex					1606:1612	the IgG1-FcγRI complex	1591:1612	the IgG1-FcγRI complex	1591:1612	Moreover, HDX-MS data evince that the Fab domain has glycan-protein binding contacts within the IgG1-FcγRI complex.					
34890647	5	32	theme	MD	1000:1001	arg1	simulations					1003:1013	MD simulations	1000:1013	MD simulations	1000:1013	MD simulations corroborate the differences in the peptide deuterium content through calculation of the percent of time that transient glycan-peptide bonds exist.					
34890647	3	33	theme	hydrogen-deuterium	684:701	arg1	spectrometry					717:728	hydrogen-deuterium exchange mass spectrometry	684:728	hydrogen-deuterium exchange mass spectrometry (HDX-MS)	684:737	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	3	33	theme	hydrogen-deuterium	684:701	arg1	HDX-MS					731:736	HDX-MS	731:736	HDX-MS	731:736	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	3	34	theme	biantennary	569:579	arg1	glycans					581:587	biantennary glycans	569:587	biantennary glycans	569:587	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	7	35	from	region	1460:1465	arg1	IgG1					1493:1496	isolated and complexed IgG1	1470:1496	isolated and complexed IgG1	1470:1496	The results also indicate that intramolecular glycan-protein bonds stabilize the Fc region in isolated and complexed IgG1.					
34890647	8	36	theme	HDX-MS	1509:1514	arg1	data					1516:1519	HDX-MS data	1509:1519	HDX-MS data	1509:1519	Moreover, HDX-MS data evince that the Fab domain has glycan-protein binding contacts within the IgG1-FcγRI complex.					
34890647	4	37	theme	FcγRIa	957:962	arg1	peptides					933:940	peptic peptides	926:940	peptic peptides of Fab, Fc and FcγRIa	926:962	For each glycoform of the IgG1-FcγRIa complex peptic peptides of Fab, Fc and FcγRIa report distinct H/D exchange rates.					
34890647	3	38	theme	exchange	703:710	arg1	spectrometry					717:728	hydrogen-deuterium exchange mass spectrometry	684:728	hydrogen-deuterium exchange mass spectrometry (HDX-MS)	684:737	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	3	38	theme	exchange	703:710	arg1	HDX-MS					731:736	HDX-MS	731:736	HDX-MS	731:736	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	6	39	theme	IgG1	1309:1312	arg1	glycans					1314:1320	the IgG1 glycans	1305:1320	the IgG1 glycans	1305:1320	These results indicate that stability of IgG1-FcγRIa complexes correlate with the presence of intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa.					
34890647	0	40	theme	Glycoprotein	126:137	arg1	Bonds					139:143	Intermolecular Glycoprotein Bonds	111:143	Intermolecular Glycoprotein Bonds	111:143	HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1-FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds.					
34890647	5	41	theme	time	1114:1117	arg1	percent					1103:1109	the percent	1099:1109	the percent of time	1099:1117	MD simulations corroborate the differences in the peptide deuterium content through calculation of the percent of time that transient glycan-peptide bonds exist.					
34890647	5	42	theme	peptide	1050:1056	arg1	content					1068:1074	the peptide deuterium content	1046:1074	the peptide deuterium content	1046:1074	MD simulations corroborate the differences in the peptide deuterium content through calculation of the percent of time that transient glycan-peptide bonds exist.					
34890647	1	43	theme	Fc-FcγRI	217:224	arg1	complex					233:239	the Fc-FcγRI immune complex	213:239	the Fc-FcγRI immune complex	213:239	Previous reports present different models for the stabilization of the Fc-FcγRI immune complex.					
34890647	4	44	theme	complex	918:924	arg1	glycoform					889:897	each glycoform	884:897	each glycoform of the IgG1-FcγRIa complex	884:924	For each glycoform of the IgG1-FcγRIa complex peptic peptides of Fab, Fc and FcγRIa report distinct H/D exchange rates.					
34890647	6	45	gly	glycoprotein	1271:1282	arg1	glycoprotein					1271:1282	intermolecular glycoprotein interactions	1256:1295	intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa	1256:1373	These results indicate that stability of IgG1-FcγRIa complexes correlate with the presence of intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa.					
34890647	5	46	theme	deuterium	1058:1066	arg1	content					1068:1074	the peptide deuterium content	1046:1074	the peptide deuterium content	1046:1074	MD simulations corroborate the differences in the peptide deuterium content through calculation of the percent of time that transient glycan-peptide bonds exist.					
34890647	6	47	theme	FcγRIa	1368:1373	arg1	FG-loop					1357:1363	the FG-loop	1353:1363	the FG-loop of FcγRIa	1353:1373	These results indicate that stability of IgG1-FcγRIa complexes correlate with the presence of intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa.					
34890647	2	48	theme	conserved	462:470	arg1	FG-loop					472:478	a conserved FG-loop	460:478	a conserved FG-loop (171MGKHRY176) of FcγRIa	460:503	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	2	48	theme	conserved	462:470	arg1	171MGKHRY176					481:492	171MGKHRY176	481:492	171MGKHRY176	481:492	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	3	49	theme	hoc	810:812	arg1	procedures					814:823	hoc procedures	810:823	hoc procedures	810:823	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	4	50	theme	Fc	950:951	arg1	peptides					933:940	peptic peptides	926:940	peptic peptides of Fab, Fc and FcγRIa	926:962	For each glycoform of the IgG1-FcγRIa complex peptic peptides of Fab, Fc and FcγRIa report distinct H/D exchange rates.					
34890647	6	51	theme	interactions	1284:1295	arg1	presence					1244:1251	the presence	1240:1251	the presence of intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa	1240:1373	These results indicate that stability of IgG1-FcγRIa complexes correlate with the presence of intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa.					
34890647	2	52	theme	complex	333:339	arg1	stabilization					341:353	complex stabilization	333:353	complex stabilization	333:353	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	8	53	contain	has	1548:1550	arg1	domain					1541:1546	the Fab domain	1533:1546	the Fab domain	1533:1546	Moreover, HDX-MS data evince that the Fab domain has glycan-protein binding contacts within the IgG1-FcγRI complex.					
34890647	8	53	contain	has	1548:1550	arg2	contacts					1575:1582	glycan-protein binding contacts	1552:1582	glycan-protein binding contacts within the IgG1-FcγRI complex	1552:1612	Moreover, HDX-MS data evince that the Fab domain has glycan-protein binding contacts within the IgG1-FcγRI complex.					
34890647	7	54	theme	glycan-protein	1422:1435	arg1	bonds					1437:1441	intramolecular glycan-protein bonds	1407:1441	intramolecular glycan-protein bonds	1407:1441	The results also indicate that intramolecular glycan-protein bonds stabilize the Fc region in isolated and complexed IgG1.					
34890647	4	55	theme	Fab	945:947	arg1	peptides					933:940	peptic peptides	926:940	peptic peptides of Fab, Fc and FcγRIa	926:962	For each glycoform of the IgG1-FcγRIa complex peptic peptides of Fab, Fc and FcγRIa report distinct H/D exchange rates.					
34890647	8	56	theme	Fab	1537:1539	arg1	domain					1541:1546	the Fab domain	1533:1546	the Fab domain	1533:1546	Moreover, HDX-MS data evince that the Fab domain has glycan-protein binding contacts within the IgG1-FcγRI complex.					
34890647	6	57	theme	glycoprotein	1271:1282	arg1	interactions					1284:1295	intermolecular glycoprotein interactions	1256:1295	intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa	1256:1373	These results indicate that stability of IgG1-FcγRIa complexes correlate with the presence of intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa.					
34890647	2	58	from	importance	272:281	arg1	contacts					320:327	some hydrophobic contacts	303:327	some hydrophobic contacts for complex stabilization	303:353	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	2	58	from	importance	272:281	arg1	IgG1					294:297	IgG1	294:297	IgG1	294:297	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	1	59	theme	immune	226:231	arg1	complex					233:239	the Fc-FcγRI immune complex	213:239	the Fc-FcγRI immune complex	213:239	Previous reports present different models for the stabilization of the Fc-FcγRI immune complex.					
34890647	6	60	theme	complexes	1215:1223	arg1	stability					1190:1198	stability	1190:1198	stability of IgG1-FcγRIa complexes	1190:1223	These results indicate that stability of IgG1-FcγRIa complexes correlate with the presence of intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa.					
34890647	3	61	theme	mass	712:715	arg1	spectrometry					717:728	hydrogen-deuterium exchange mass spectrometry	684:728	hydrogen-deuterium exchange mass spectrometry (HDX-MS)	684:737	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	3	61	theme	mass	712:715	arg1	HDX-MS					731:736	HDX-MS	731:736	HDX-MS	731:736	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	6	62	theme	intermolecular	1256:1269	arg1	interactions					1284:1295	intermolecular glycoprotein interactions	1256:1295	intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa	1256:1373	These results indicate that stability of IgG1-FcγRIa complexes correlate with the presence of intermolecular glycoprotein interactions between the IgG1 glycans and the 173KHR175 motif within the FG-loop of FcγRIa.					
34890647	1	63	theme	complex	233:239	arg1	stabilization					196:208	the stabilization	192:208	the stabilization of the Fc-FcγRI immune complex	192:239	Previous reports present different models for the stabilization of the Fc-FcγRI immune complex.					
34890647	2	64	theme	glycoprotein	410:421	arg1	contacts					423:430	glycoprotein contacts	410:430	glycoprotein contacts between glycans of IgG1	410:454	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	7	65	theme	intramolecular	1407:1420	arg1	bonds					1437:1441	intramolecular glycan-protein bonds	1407:1441	intramolecular glycan-protein bonds	1407:1441	The results also indicate that intramolecular glycan-protein bonds stabilize the Fc region in isolated and complexed IgG1.					
34890647	4	66	gly	glycoform	889:897	arg1	complex					918:924	the IgG1-FcγRIa complex	902:924	the IgG1-FcγRIa complex	902:924	For each glycoform of the IgG1-FcγRIa complex peptic peptides of Fab, Fc and FcγRIa report distinct H/D exchange rates.					
34890647	2	67	theme	FcγRIa	498:503	arg1	contacts					423:430	glycoprotein contacts	410:430	glycoprotein contacts between glycans of IgG1	410:454	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	2	67	theme	FcγRIa	498:503	arg1	171MGKHRY176					481:492	171MGKHRY176	481:492	171MGKHRY176	481:492	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	2	67	theme	FcγRIa	498:503	arg1	FG-loop					472:478	a conserved FG-loop	460:478	a conserved FG-loop (171MGKHRY176) of FcγRIa	460:503	Although accord exists on the importance of L235 in IgG1 and some hydrophobic contacts for complex stabilization, discord exists regarding the existence of stabilizing glycoprotein contacts between glycans of IgG1 and a conserved FG-loop (171MGKHRY176) of FcγRIa.					
34890647	3	68	theme	α2,6-N-acetylneuraminic	630:652	arg1	terminations					654:665	α2,6-N-acetylneuraminic terminations	630:665	α2,6-N-acetylneuraminic terminations	630:665	Complexes formed from the FcγRIa receptor and IgG1s containing biantennary glycans with N-acetylglucosamine, galactose, and α2,6-N-acetylneuraminic terminations were measured by hydrogen-deuterium exchange mass spectrometry (HDX-MS), classified for dissimilarity with Welch's ANOVA and Games-Howell post hoc procedures, and modeled with molecular dynamics (MD) simulations.					
34890647	1	69	theme	Previous	146:153	arg1	reports					155:161	Previous reports	146:161	Previous reports	146:161	Previous reports present different models for the stabilization of the Fc-FcγRI immune complex.					
33051009	6	0	theme	surface	1037:1043	arg1	methodology					1045:1055	response surface methodology	1028:1055	response surface methodology	1028:1055	With a higher conversion yield from quercetin to QGs (60-92%), the optimum conditions for producing QG2' were examined under various pH and sucrose concentrations by response surface methodology.					
33051009	5	1	theme	quercetin	729:737	arg1	QG2					698:700	The newly synthesized QG2	676:700	The newly synthesized QG2'	676:701	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	5	1	theme	quercetin	729:737	arg1	isomaltoside					739:750	a novel quercetin isomaltoside	721:750	a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra	721:859	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	1	2	theme	phenolics	271:279	arg1	transglycosylation					229:246	transglycosylation	229:246	transglycosylation of many flavonoids and phenolics	229:279	Amylosucrase (ASase, EC.4.2.1.4) is well-known for its distinguishable property of transglycosylation of many flavonoids and phenolics.					
33051009	5	3	theme	α-1,6	760:764	arg1	linkage					766:772	an α-1,6 linkage	757:772	an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra	757:859	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	6	4	theme	conversion	876:885	arg1	yield					887:891	a higher conversion yield	867:891	a higher conversion yield from quercetin to QGs (60-92%)	867:922	With a higher conversion yield from quercetin to QGs (60-92%), the optimum conditions for producing QG2' were examined under various pH and sucrose concentrations by response surface methodology.					
33051009	2	5	theme	poor	361:364	arg1	solubility					366:375	poor solubility	361:375	poor solubility	361:375	Quercetin has diverse biological functions, however, its use is limited due to poor solubility and bioavailability.					
33051009	9	6	theme	buffer	1373:1378	arg1	salts					1380:1384	buffer salts	1373:1384	buffer salts	1373:1384	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	0	7	theme	novel	107:111	arg1	α-d-isomaltoside					128:143	novel quercetin 4'-O-α-d-isomaltoside	107:143	novel quercetin 4'-O-α-d-isomaltoside	107:143	Amylosucrase from Deinococcus geothermalis can be modulated under different reaction conditions to produce novel quercetin 4'-O-α-d-isomaltoside.					
33051009	6	8	theme	higher	869:874	arg1	yield					887:891	a higher conversion yield	867:891	a higher conversion yield from quercetin to QGs (60-92%)	867:922	With a higher conversion yield from quercetin to QGs (60-92%), the optimum conditions for producing QG2' were examined under various pH and sucrose concentrations by response surface methodology.					
33051009	1	9	dep	Amylosucrase	146:157	arg1	EC.4.2.1.4					167:176	EC.4.2.1.4	167:176	EC.4.2.1.4	167:176	Amylosucrase (ASase, EC.4.2.1.4) is well-known for its distinguishable property of transglycosylation of many flavonoids and phenolics.					
33051009	1	9	dep	Amylosucrase	146:157	arg1	ASase					160:164	ASase	160:164	ASase	160:164	Amylosucrase (ASase, EC.4.2.1.4) is well-known for its distinguishable property of transglycosylation of many flavonoids and phenolics.					
33051009	6	10	theme	optimum	929:935	arg1	conditions					937:946	the optimum conditions	925:946	the optimum conditions for producing QG2'	925:965	With a higher conversion yield from quercetin to QGs (60-92%), the optimum conditions for producing QG2' were examined under various pH and sucrose concentrations by response surface methodology.					
33051009	7	11	theme	sucrose	1135:1141	arg1	1000-1500 mM					1159:1170	1000-1500 mM	1159:1170	1000-1500 mM	1159:1170	QG2' was predominantly produced under acidic conditions (pH 5.0) and at high sucrose concentrations (1000-1500 mM).					
33051009	7	11	theme	sucrose	1135:1141	arg1	concentrations					1143:1156	high sucrose concentrations	1130:1156	high sucrose concentrations (1000-1500 mM)	1130:1171	QG2' was predominantly produced under acidic conditions (pH 5.0) and at high sucrose concentrations (1000-1500 mM).					
33051009	11	12	theme	reaction	1781:1788	arg1	conditions					1790:1799	reaction conditions	1781:1799	reaction conditions	1781:1799	Taken together, our study demonstrated that ASase can be used to synthesize unusually branched flavonoid glycosides from flavonol aglycones with clear patterns by modulating reaction conditions.					
33051009	4	13	from	sucrose	667:673	arg1	diglucosides					602:613	diglucosides	602:613	diglucosides (QG2 and QG2')	602:628	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	13	from	sucrose	667:673	arg1	monoglucosides					580:593	quercetin monoglucosides	570:593	quercetin monoglucosides (QG1)	570:599	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	13	from	sucrose	667:673	arg1	triglucoside					635:646	triglucoside	635:646	triglucoside from quercetin and sucrose	635:673	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	13	from	sucrose	667:673	arg1	QG2					616:618	QG2	616:618	QG2	616:618	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	13	from	sucrose	667:673	arg1	QG1					596:598	QG1	596:598	QG1	596:598	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	13	from	sucrose	667:673	arg1	QG2					624:626	QG2	624:626	QG2	624:626	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	2	14	contain	has	292:294	arg1	Quercetin					282:290	Quercetin	282:290	Quercetin	282:290	Quercetin has diverse biological functions, however, its use is limited due to poor solubility and bioavailability.					
33051009	2	14	contain	has	292:294	arg2	functions					315:323	diverse biological functions	296:323	diverse biological functions	296:323	Quercetin has diverse biological functions, however, its use is limited due to poor solubility and bioavailability.					
33051009	0	15	theme	4'-O-	123:127	arg1	α-d-isomaltoside					128:143	novel quercetin 4'-O-α-d-isomaltoside	107:143	novel quercetin 4'-O-α-d-isomaltoside	107:143	Amylosucrase from Deinococcus geothermalis can be modulated under different reaction conditions to produce novel quercetin 4'-O-α-d-isomaltoside.					
33051009	6	16	theme	various	987:993	arg1	pH					995:996	various pH and sucrose concentrations	987:1023	pH	995:996	With a higher conversion yield from quercetin to QGs (60-92%), the optimum conditions for producing QG2' were examined under various pH and sucrose concentrations by response surface methodology.					
33051009	2	17	theme	biological	304:313	arg1	functions					315:323	diverse biological functions	296:323	diverse biological functions	296:323	Quercetin has diverse biological functions, however, its use is limited due to poor solubility and bioavailability.					
33051009	6	18	theme	sucrose	1002:1008	arg1	concentrations					1010:1023	various pH and sucrose concentrations	987:1023	concentrations	1010:1023	With a higher conversion yield from quercetin to QGs (60-92%), the optimum conditions for producing QG2' were examined under various pH and sucrose concentrations by response surface methodology.					
33051009	3	19	theme	conditional	456:466	arg1	preference					468:477	conditional preference	456:477	conditional preference for producing unusual quercetin glucosides (QGs)	456:526	ASase derived from Deinococcus geothermalis (DGAS) showed conditional preference for producing unusual quercetin glucosides (QGs).					
33051009	0	20	theme	quercetin	113:121	arg1	α-d-isomaltoside					128:143	novel quercetin 4'-O-α-d-isomaltoside	107:143	novel quercetin 4'-O-α-d-isomaltoside	107:143	Amylosucrase from Deinococcus geothermalis can be modulated under different reaction conditions to produce novel quercetin 4'-O-α-d-isomaltoside.					
33051009	5	21	from	-OH	790:792	arg1	quercetin					804:812	quercetin	804:812	quercetin	804:812	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	10	22	theme	transglycosylations	1533:1551	arg1	rationale					1512:1520	A rationale	1510:1520	A rationale of unusual transglycosylations	1510:1551	A rationale of unusual transglycosylations was demonstrated with a molecular docking simulation.					
33051009	11	23	with	glycosides	1712:1721	arg1	patterns					1758:1765	clear patterns	1752:1765	clear patterns	1752:1765	Taken together, our study demonstrated that ASase can be used to synthesize unusually branched flavonoid glycosides from flavonol aglycones with clear patterns by modulating reaction conditions.					
33051009	2	24	theme	diverse	296:302	arg1	functions					315:323	diverse biological functions	296:323	diverse biological functions	296:323	Quercetin has diverse biological functions, however, its use is limited due to poor solubility and bioavailability.					
33051009	4	25	from	quercetin	653:661	arg1	diglucosides					602:613	diglucosides	602:613	diglucosides (QG2 and QG2')	602:628	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	25	from	quercetin	653:661	arg1	monoglucosides					580:593	quercetin monoglucosides	570:593	quercetin monoglucosides (QG1)	570:599	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	25	from	quercetin	653:661	arg1	triglucoside					635:646	triglucoside	635:646	triglucoside from quercetin and sucrose	635:673	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	25	from	quercetin	653:661	arg1	QG2					616:618	QG2	616:618	QG2	616:618	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	25	from	quercetin	653:661	arg1	QG1					596:598	QG1	596:598	QG1	596:598	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	25	from	quercetin	653:661	arg1	QG2					624:626	QG2	624:626	QG2	624:626	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	5	26	theme	nuclear	826:832	arg1	resonance					843:851	mass and nuclear magnetic resonance	817:851	resonance	843:851	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	1	27	gly	transglycosylation	229:246	arg1	flavonoids					256:265	many flavonoids	251:265	many flavonoids	251:265	Amylosucrase (ASase, EC.4.2.1.4) is well-known for its distinguishable property of transglycosylation of many flavonoids and phenolics.					
33051009	1	27	gly	transglycosylation	229:246	arg1	phenolics					271:279	phenolics	271:279	phenolics	271:279	Amylosucrase (ASase, EC.4.2.1.4) is well-known for its distinguishable property of transglycosylation of many flavonoids and phenolics.					
33051009	5	28	theme	magnetic	834:841	arg1	resonance					843:851	mass and nuclear magnetic resonance	817:851	resonance	843:851	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	11	29	theme	branched	1693:1700	arg1	glycosides					1712:1721	unusually branched flavonoid glycosides	1683:1721	unusually branched flavonoid glycosides from flavonol aglycones with clear patterns	1683:1765	Taken together, our study demonstrated that ASase can be used to synthesize unusually branched flavonoid glycosides from flavonol aglycones with clear patterns by modulating reaction conditions.					
33051009	11	30	theme	flavonol	1728:1735	arg1	aglycones					1737:1745	flavonol aglycones	1728:1745	flavonol aglycones	1728:1745	Taken together, our study demonstrated that ASase can be used to synthesize unusually branched flavonoid glycosides from flavonol aglycones with clear patterns by modulating reaction conditions.					
33051009	11	31	from	aglycones	1737:1745	arg1	glycosides					1712:1721	unusually branched flavonoid glycosides	1683:1721	unusually branched flavonoid glycosides from flavonol aglycones with clear patterns	1683:1765	Taken together, our study demonstrated that ASase can be used to synthesize unusually branched flavonoid glycosides from flavonol aglycones with clear patterns by modulating reaction conditions.					
33051009	11	32	theme	clear	1752:1756	arg1	patterns					1758:1765	clear patterns	1752:1765	clear patterns	1752:1765	Taken together, our study demonstrated that ASase can be used to synthesize unusually branched flavonoid glycosides from flavonol aglycones with clear patterns by modulating reaction conditions.					
33051009	5	33	theme	resonance	843:851	arg1	spectra					853:859	mass and nuclear magnetic resonance spectra	817:859	mass and nuclear magnetic resonance spectra	817:859	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	4	34	theme	quercetin	570:578	arg1	monoglucosides					580:593	quercetin monoglucosides	570:593	quercetin monoglucosides (QG1)	570:599	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	34	theme	quercetin	570:578	arg1	QG1					596:598	QG1	596:598	QG1	596:598	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	3	35	theme	unusual	493:499	arg1	QGs					523:525	QGs	523:525	QGs	523:525	ASase derived from Deinococcus geothermalis (DGAS) showed conditional preference for producing unusual quercetin glucosides (QGs).					
33051009	3	35	theme	unusual	493:499	arg1	glucosides					511:520	unusual quercetin glucosides	493:520	unusual quercetin glucosides (QGs)	493:526	ASase derived from Deinococcus geothermalis (DGAS) showed conditional preference for producing unusual quercetin glucosides (QGs).					
33051009	9	36	theme	considerable	1289:1300	arg1	differences					1302:1312	considerable differences	1289:1312	considerable differences of transglycosylation velocities	1289:1345	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	6	37	theme	response	1028:1035	arg1	methodology					1045:1055	response surface methodology	1028:1055	response surface methodology	1028:1055	With a higher conversion yield from quercetin to QGs (60-92%), the optimum conditions for producing QG2' were examined under various pH and sucrose concentrations by response surface methodology.					
33051009	3	38	theme	quercetin	501:509	arg1	QGs					523:525	QGs	523:525	QGs	523:525	ASase derived from Deinococcus geothermalis (DGAS) showed conditional preference for producing unusual quercetin glucosides (QGs).					
33051009	3	38	theme	quercetin	501:509	arg1	glucosides					511:520	unusual quercetin glucosides	493:520	unusual quercetin glucosides (QGs)	493:526	ASase derived from Deinococcus geothermalis (DGAS) showed conditional preference for producing unusual quercetin glucosides (QGs).					
33051009	7	39	theme	acidic	1096:1101	arg1	pH					1115:1116	pH 5.0	1115:1120	pH 5.0	1115:1120	QG2' was predominantly produced under acidic conditions (pH 5.0) and at high sucrose concentrations (1000-1500 mM).					
33051009	7	39	theme	acidic	1096:1101	arg1	conditions					1103:1112	acidic conditions	1096:1112	acidic conditions (pH 5.0)	1096:1121	QG2' was predominantly produced under acidic conditions (pH 5.0) and at high sucrose concentrations (1000-1500 mM).					
33051009	4	40	dep	diglucosides	602:613	arg1	diglucosides					602:613	diglucosides	602:613	diglucosides (QG2 and QG2')	602:628	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	40	dep	diglucosides	602:613	arg1	QG2					624:626	QG2	624:626	QG2	624:626	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	40	dep	diglucosides	602:613	arg1	QG2					616:618	QG2	616:618	QG2	616:618	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	10	41	theme	docking	1587:1593	arg1	simulation					1595:1604	a molecular docking simulation	1575:1604	a molecular docking simulation	1575:1604	A rationale of unusual transglycosylations was demonstrated with a molecular docking simulation.					
33051009	10	42	theme	unusual	1525:1531	arg1	transglycosylations					1533:1551	unusual transglycosylations	1525:1551	unusual transglycosylations	1525:1551	A rationale of unusual transglycosylations was demonstrated with a molecular docking simulation.					
33051009	11	43	used	used	1664:1667	arg2	ASase					1651:1655	ASase	1651:1655	ASase	1651:1655	Taken together, our study demonstrated that ASase can be used to synthesize unusually branched flavonoid glycosides from flavonol aglycones with clear patterns by modulating reaction conditions.					
33051009	9	44	theme	higher	1424:1429	arg1	performance					1439:1449	a 3.9-fold higher overall performance	1413:1449	a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5	1413:1487	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	9	44	theme	higher	1424:1429	arg1	'm					1458:1459	kcat/K'm	1452:1459	kcat/K'm	1452:1459	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	1	45	theme	transglycosylation	229:246	arg1	property					217:224	its distinguishable property	197:224	its distinguishable property of transglycosylation of many flavonoids and phenolics	197:279	Amylosucrase (ASase, EC.4.2.1.4) is well-known for its distinguishable property of transglycosylation of many flavonoids and phenolics.					
33051009	3	46	theme	Deinococcus	417:427	arg1	geothermalis					429:440	Deinococcus geothermalis	417:440	Deinococcus geothermalis (DGAS)	417:447	ASase derived from Deinococcus geothermalis (DGAS) showed conditional preference for producing unusual quercetin glucosides (QGs).					
33051009	3	46	theme	Deinococcus	417:427	arg1	DGAS					443:446	DGAS	443:446	DGAS	443:446	ASase derived from Deinococcus geothermalis (DGAS) showed conditional preference for producing unusual quercetin glucosides (QGs).					
33051009	7	47	theme	high	1130:1133	arg1	1000-1500 mM					1159:1170	1000-1500 mM	1159:1170	1000-1500 mM	1159:1170	QG2' was predominantly produced under acidic conditions (pH 5.0) and at high sucrose concentrations (1000-1500 mM).					
33051009	7	47	theme	high	1130:1133	arg1	concentrations					1143:1156	high sucrose concentrations	1130:1156	high sucrose concentrations (1000-1500 mM)	1130:1171	QG2' was predominantly produced under acidic conditions (pH 5.0) and at high sucrose concentrations (1000-1500 mM).					
33051009	9	48	theme	Kinetic	1254:1260	arg1	evaluations					1262:1272	Kinetic evaluations	1254:1272	Kinetic evaluations	1254:1272	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	9	49	theme	overall	1431:1437	arg1	performance					1439:1449	a 3.9-fold higher overall performance	1413:1449	a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5	1413:1487	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	9	49	theme	overall	1431:1437	arg1	'm					1458:1459	kcat/K'm	1452:1459	kcat/K'm	1452:1459	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	9	50	theme	velocities	1336:1345	arg1	differences					1302:1312	considerable differences	1289:1312	considerable differences of transglycosylation velocities	1289:1345	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	6	51	from	quercetin	898:906	arg1	yield					887:891	a higher conversion yield	867:891	a higher conversion yield from quercetin to QGs (60-92%)	867:922	With a higher conversion yield from quercetin to QGs (60-92%), the optimum conditions for producing QG2' were examined under various pH and sucrose concentrations by response surface methodology.					
33051009	11	52	theme	flavonoid	1702:1710	arg1	glycosides					1712:1721	unusually branched flavonoid glycosides	1683:1721	unusually branched flavonoid glycosides from flavonol aglycones with clear patterns	1683:1765	Taken together, our study demonstrated that ASase can be used to synthesize unusually branched flavonoid glycosides from flavonol aglycones with clear patterns by modulating reaction conditions.					
33051009	4	53	theme	QGs	556:558	arg1	QGs					556:558	QGs	556:558	QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose	556:673	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	53	theme	QGs	556:558	arg1	monoglucosides					580:593	quercetin monoglucosides	570:593	quercetin monoglucosides (QG1)	570:599	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	53	theme	QGs	556:558	arg1	variety					545:551	a variety	543:551	a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose	543:673	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	53	theme	QGs	556:558	arg1	triglucoside					635:646	triglucoside	635:646	triglucoside from quercetin and sucrose	635:673	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	4	53	theme	QGs	556:558	arg1	diglucosides					602:613	diglucosides	602:613	diglucosides (QG2 and QG2')	602:628	DGAS produced a variety of QGs including quercetin monoglucosides (QG1), diglucosides (QG2 and QG2'), and triglucoside from quercetin and sucrose.					
33051009	3	54	attach	derived	404:410	arg1	geothermalis					429:440	Deinococcus geothermalis	417:440	Deinococcus geothermalis (DGAS)	417:447	ASase derived from Deinococcus geothermalis (DGAS) showed conditional preference for producing unusual quercetin glucosides (QGs).					
33051009	3	54	attach	derived	404:410	arg2	ASase					398:402	ASase	398:402	ASase derived from Deinococcus geothermalis (DGAS)	398:447	ASase derived from Deinococcus geothermalis (DGAS) showed conditional preference for producing unusual quercetin glucosides (QGs).					
33051009	3	54	attach	derived	404:410	arg1	DGAS					443:446	DGAS	443:446	DGAS	443:446	ASase derived from Deinococcus geothermalis (DGAS) showed conditional preference for producing unusual quercetin glucosides (QGs).					
33051009	8	55	theme	consecutive	1227:1237	arg1	glycosylation					1239:1251	consecutive glycosylation	1227:1251	consecutive glycosylation	1227:1251	In contrast, QG1 was generated as an intermediate of consecutive glycosylation.					
33051009	5	56	theme	novel	723:727	arg1	QG2					698:700	The newly synthesized QG2	676:700	The newly synthesized QG2'	676:701	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	5	56	theme	novel	723:727	arg1	isomaltoside					739:750	a novel quercetin isomaltoside	721:750	a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra	721:859	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	5	57	theme	synthesized	686:696	arg1	isomaltoside					739:750	a novel quercetin isomaltoside	721:750	a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra	721:859	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	5	57	theme	synthesized	686:696	arg1	QG2					698:700	The newly synthesized QG2	676:700	The newly synthesized QG2'	676:701	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	10	58	theme	molecular	1577:1585	arg1	simulation					1595:1604	a molecular docking simulation	1575:1604	a molecular docking simulation	1575:1604	A rationale of unusual transglycosylations was demonstrated with a molecular docking simulation.					
33051009	9	59	theme	kcat/K	1452:1457	arg1	performance					1439:1449	a 3.9-fold higher overall performance	1413:1449	a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5	1413:1487	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	9	59	theme	kcat/K	1452:1457	arg1	'm					1458:1459	kcat/K'm	1452:1459	kcat/K'm	1452:1459	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	1	60	theme	many	251:254	arg1	flavonoids					256:265	many flavonoids	251:265	many flavonoids	251:265	Amylosucrase (ASase, EC.4.2.1.4) is well-known for its distinguishable property of transglycosylation of many flavonoids and phenolics.					
33051009	0	61	theme	reaction	76:83	arg1	conditions					85:94	different reaction conditions	66:94	different reaction conditions	66:94	Amylosucrase from Deinococcus geothermalis can be modulated under different reaction conditions to produce novel quercetin 4'-O-α-d-isomaltoside.					
33051009	5	62	theme	mass	817:820	arg1	resonance					843:851	mass and nuclear magnetic resonance	817:851	resonance	843:851	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	5	63	with	isomaltoside	739:750	arg1	linkage					766:772	an α-1,6 linkage	757:772	an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra	757:859	The newly synthesized QG2' was recognized as a novel quercetin isomaltoside with an α-1,6 linkage branched at the -OH of C4' in quercetin by mass and nuclear magnetic resonance spectra.					
33051009	1	64	theme	distinguishable	201:215	arg1	property					217:224	its distinguishable property	197:224	its distinguishable property of transglycosylation of many flavonoids and phenolics	197:279	Amylosucrase (ASase, EC.4.2.1.4) is well-known for its distinguishable property of transglycosylation of many flavonoids and phenolics.					
33051009	9	65	dep	pH	1366:1367	arg1	the					1362:1364	the	1362:1364	the	1362:1364	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	9	66	theme	reaction	1393:1400	arg1	pH					1366:1367	pH	1366:1367	pH	1366:1367	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	9	66	theme	reaction	1393:1400	arg1	salts					1380:1384	buffer salts	1373:1384	buffer salts	1373:1384	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
33051009	1	67	theme	flavonoids	256:265	arg1	transglycosylation					229:246	transglycosylation	229:246	transglycosylation of many flavonoids and phenolics	229:279	Amylosucrase (ASase, EC.4.2.1.4) is well-known for its distinguishable property of transglycosylation of many flavonoids and phenolics.					
33051009	0	68	theme	different	66:74	arg1	conditions					85:94	different reaction conditions	66:94	different reaction conditions	66:94	Amylosucrase from Deinococcus geothermalis can be modulated under different reaction conditions to produce novel quercetin 4'-O-α-d-isomaltoside.					
33051009	0	69	from	geothermalis	30:41	arg1	Amylosucrase					0:11	Amylosucrase	0:11	Amylosucrase from Deinococcus geothermalis	0:41	Amylosucrase from Deinococcus geothermalis can be modulated under different reaction conditions to produce novel quercetin 4'-O-α-d-isomaltoside.					
33051009	8	70	theme	glycosylation	1239:1251	arg1	intermediate					1211:1222	an intermediate	1208:1222	an intermediate of consecutive glycosylation	1208:1251	In contrast, QG1 was generated as an intermediate of consecutive glycosylation.					
33051009	8	70	theme	glycosylation	1239:1251	arg1	QG1					1187:1189	QG1	1187:1189	QG1	1187:1189	In contrast, QG1 was generated as an intermediate of consecutive glycosylation.					
33051009	9	71	theme	transglycosylation	1317:1334	arg1	velocities					1336:1345	transglycosylation velocities	1317:1345	transglycosylation velocities	1317:1345	Kinetic evaluations indicated that considerable differences of transglycosylation velocities were caused by the pH and buffer salts of the reaction, which had a 3.9-fold higher overall performance (kcat/K'm) of generating QG2' at pH 5 compared to at pH 7.					
34009123	2	0	theme	function	360:367	arg1	loss					311:314	loss	311:314	loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression	311:421	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	5	1	theme	sepsis	797:802	arg1	form					789:792	a less severe form	775:792	a less severe form of sepsis	775:802	Systemic infection with an Ig-shaped microbiota inoculum elicited a less severe form of sepsis compared to infection with non-Ig-shaped microbiota.					
34009123	7	2	theme	Ggta1	1092:1096	arg1	deletion					1098:1105	Ggta1 deletion	1092:1105	Ggta1 deletion in mice	1092:1113	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	5	3	with	infection	816:824	arg1	microbiota					845:854	non-Ig-shaped microbiota	831:854	non-Ig-shaped microbiota	831:854	Systemic infection with an Ig-shaped microbiota inoculum elicited a less severe form of sepsis compared to infection with non-Ig-shaped microbiota.					
34009123	4	4	theme	αGal-expressing	676:690	arg1	bacteria					692:699	αGal-expressing bacteria	676:699	αGal-expressing bacteria	676:699	This occurred via an immunoglobulin (Ig)-dependent mechanism, associated with targeting of αGal-expressing bacteria by IgA.					
34009123	7	5	from	deletion	1098:1105	arg1	mice					1110:1113	mice	1110:1113	mice	1110:1113	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	3	6	theme	gut	569:571	arg1	microbiota					573:582	the gut microbiota	565:582	the gut microbiota	565:582	We found that Ggta1 deletion shaped the composition of the gut microbiota.					
34009123	7	7	theme	fitness	980:986	arg1	cost					988:991	the fitness cost	976:991	the fitness cost imposed by bacterial sepsis	976:1019	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	3	8	theme	Ggta1	524:528	arg1	deletion					530:537	Ggta1 deletion	524:537	Ggta1 deletion	524:537	We found that Ggta1 deletion shaped the composition of the gut microbiota.					
34009123	2	9	used	used	246:249	arg2	we					243:244	we	243:244	we	243:244	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	1	10	theme	high	125:128	arg1	pressure					140:147	relatively high selection pressure	114:147	relatively high selection pressure	114:147	Genes encoding glycosyltransferases can be under relatively high selection pressure, likely due to the involvement of the glycans synthesized in host-microbe interactions.					
34009123	2	11	theme	model	275:279	arg1	system					281:286	an experimental model system	259:286	an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions	259:458	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	2	11	theme	model	275:279	arg1	mice					251:254	mice	251:254	mice	251:254	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	2	12	theme	glycan	405:410	arg1	expression					412:421	Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression	373:421	Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression	373:421	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	7	13	theme	GGTA1	1165:1169	arg1	mutations					1188:1196	GGTA1 loss-of-function mutations	1165:1196	GGTA1 loss-of-function mutations	1165:1196	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	2	14	theme	experimental	262:273	arg1	system					281:286	an experimental model system	259:286	an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions	259:458	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	2	14	theme	experimental	262:273	arg1	mice					251:254	mice	251:254	mice	251:254	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	5	15	with	infection	718:726	arg1	inoculum					757:764	an Ig-shaped microbiota inoculum	733:764	an Ig-shaped microbiota inoculum	733:764	Systemic infection with an Ig-shaped microbiota inoculum elicited a less severe form of sepsis compared to infection with non-Ig-shaped microbiota.					
34009123	5	16	theme	non-Ig-shaped	831:843	arg1	microbiota					845:854	non-Ig-shaped microbiota	831:854	non-Ig-shaped microbiota	831:854	Systemic infection with an Ig-shaped microbiota inoculum elicited a less severe form of sepsis compared to infection with non-Ig-shaped microbiota.					
34009123	2	17	theme	αGal	399:402	arg1	expression					412:421	Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression	373:421	Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression	373:421	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	0	18	theme	Glycan-based	0:11	arg1	shaping					13:19	Glycan-based shaping	0:19	Glycan-based shaping of the microbiota during primate evolution	0:62	Glycan-based shaping of the microbiota during primate evolution.					
34009123	7	19	theme	mutations	1188:1196	arg1	frequency					1152:1160	the frequency	1148:1160	the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans	1148:1243	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	1	20	theme	glycans	187:193	arg1	involvement					168:178	the involvement	164:178	the involvement of the glycans synthesized in host-microbe interactions	164:234	Genes encoding glycosyltransferases can be under relatively high selection pressure, likely due to the involvement of the glycans synthesized in host-microbe interactions.					
34009123	3	21	theme	microbiota	573:582	arg1	composition					550:560	the composition	546:560	the composition of the gut microbiota	546:582	We found that Ggta1 deletion shaped the composition of the gut microbiota.					
34009123	7	22	theme	ancestral	1201:1209	arg1	primates					1211:1218	ancestral primates	1201:1218	ancestral primates that gave rise to humans	1201:1243	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	6	23	theme	αGal	899:902	arg1	absence					883:889	the absence	879:889	the absence of host αGal	879:902	This suggests that in the absence of host αGal, antibodies can shape the microbiota towards lower pathogenicity.					
34009123	2	24	theme	host-microbiota	431:445	arg1	interactions					447:458	host-microbiota interactions	431:458	host-microbiota interactions	431:458	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	7	25	theme	loss-of-function	1171:1186	arg1	mutations					1188:1196	GGTA1 loss-of-function mutations	1165:1196	GGTA1 loss-of-function mutations	1165:1196	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	5	26	theme	Ig-shaped	736:744	arg1	inoculum					757:764	an Ig-shaped microbiota inoculum	733:764	an Ig-shaped microbiota inoculum	733:764	Systemic infection with an Ig-shaped microbiota inoculum elicited a less severe form of sepsis compared to infection with non-Ig-shaped microbiota.					
34009123	6	27	theme	host	894:897	arg1	αGal					899:902	host αGal	894:902	host αGal	894:902	This suggests that in the absence of host αGal, antibodies can shape the microbiota towards lower pathogenicity.					
34009123	5	28	theme	microbiota	746:755	arg1	inoculum					757:764	an Ig-shaped microbiota inoculum	733:764	an Ig-shaped microbiota inoculum	733:764	Systemic infection with an Ig-shaped microbiota inoculum elicited a less severe form of sepsis compared to infection with non-Ig-shaped microbiota.					
34009123	4	29	theme	bacteria	692:699	arg1	targeting					663:671	targeting	663:671	targeting of αGal-expressing bacteria by IgA	663:706	This occurred via an immunoglobulin (Ig)-dependent mechanism, associated with targeting of αGal-expressing bacteria by IgA.					
34009123	1	30	theme	host-microbe	210:221	arg1	interactions					223:234	host-microbe interactions	210:234	host-microbe interactions	210:234	Genes encoding glycosyltransferases can be under relatively high selection pressure, likely due to the involvement of the glycans synthesized in host-microbe interactions.					
34009123	2	31	theme	primate	491:497	arg1	evolution					499:507	primate evolution	491:507	primate evolution	491:507	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	0	32	theme	microbiota	28:37	arg1	shaping					13:19	Glycan-based shaping	0:19	Glycan-based shaping of the microbiota during primate evolution	0:62	Glycan-based shaping of the microbiota during primate evolution.					
34009123	2	33	theme	expression	412:421	arg1	loss					311:314	loss	311:314	loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression	311:421	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	7	34	theme	microbiota	1062:1071	arg1	pathogenicity					1073:1085	microbiota pathogenicity	1062:1085	microbiota pathogenicity	1062:1085	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	7	35	theme	observed	1040:1047	arg1	reduction					1049:1057	the observed reduction	1036:1057	the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice	1036:1113	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	2	36	theme	GGTA1	353:357	arg1	function					360:367	α-1,3-galactosyltransferase gene (GGTA1) function	319:367	α-1,3-galactosyltransferase gene (GGTA1) function	319:367	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	0	37	theme	primate	46:52	arg1	evolution					54:62	primate evolution	46:62	primate evolution	46:62	Glycan-based shaping of the microbiota during primate evolution.					
34009123	1	38	theme	selection	130:138	arg1	pressure					140:147	relatively high selection pressure	114:147	relatively high selection pressure	114:147	Genes encoding glycosyltransferases can be under relatively high selection pressure, likely due to the involvement of the glycans synthesized in host-microbe interactions.					
34009123	7	39	from	frequency	1152:1160	arg1	primates					1211:1218	ancestral primates	1201:1218	ancestral primates that gave rise to humans	1201:1243	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	5	40	theme	Systemic	709:716	arg1	infection					718:726	Systemic infection	709:726	Systemic infection with an Ig-shaped microbiota inoculum	709:764	Systemic infection with an Ig-shaped microbiota inoculum elicited a less severe form of sepsis compared to infection with non-Ig-shaped microbiota.					
34009123	2	41	theme	gene	347:350	arg1	function					360:367	α-1,3-galactosyltransferase gene (GGTA1) function	319:367	α-1,3-galactosyltransferase gene (GGTA1) function	319:367	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	7	42	from	reduction	1049:1057	arg1	pathogenicity					1073:1085	microbiota pathogenicity	1062:1085	microbiota pathogenicity	1062:1085	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	2	43	theme	α-1,3-galactosyltransferase	319:345	arg1	function					360:367	α-1,3-galactosyltransferase gene (GGTA1) function	319:367	α-1,3-galactosyltransferase gene (GGTA1) function	319:367	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	5	44	theme	severe	782:787	arg1	form					789:792	a less severe form	775:792	a less severe form of sepsis	775:802	Systemic infection with an Ig-shaped microbiota inoculum elicited a less severe form of sepsis compared to infection with non-Ig-shaped microbiota.					
34009123	2	45	theme	Galα1-3Galβ1-4GlcNAcβ1-R	373:396	arg1	expression					412:421	Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression	373:421	Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression	373:421	Here, we used mice as an experimental model system to investigate whether loss of α-1,3-galactosyltransferase gene (GGTA1) function and Galα1-3Galβ1-4GlcNAcβ1-R (αGal) glycan expression affects host-microbiota interactions, as might have occurred during primate evolution.					
34009123	6	46	theme	lower	949:953	arg1	pathogenicity					955:967	lower pathogenicity	949:967	lower pathogenicity	949:967	This suggests that in the absence of host αGal, antibodies can shape the microbiota towards lower pathogenicity.					
34009123	7	47	theme	bacterial	1004:1012	arg1	sepsis					1014:1019	bacterial sepsis	1004:1019	bacterial sepsis	1004:1019	Given the fitness cost imposed by bacterial sepsis, we infer that the observed reduction in microbiota pathogenicity upon Ggta1 deletion in mice may have contributed to increase the frequency of GGTA1 loss-of-function mutations in ancestral primates that gave rise to humans.					
34009123	4	48	theme	-dependent	625:634	arg1	mechanism					636:644	an immunoglobulin (Ig)-dependent mechanism	603:644	an immunoglobulin (Ig)-dependent mechanism	603:644	This occurred via an immunoglobulin (Ig)-dependent mechanism, associated with targeting of αGal-expressing bacteria by IgA.					
34903166	10	0	theme	root	1974:1977	arg1	tip					1979:1981	root tip	1974:1981	root tip	1974:1981	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	12	1	theme	knock-out	2397:2405	arg1	mutants					2407:2413	Hyp-GALT knock-out mutants	2388:2413	Hyp-GALT knock-out mutants	2388:2413	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	13	2	theme	polysaccharides	2524:2538	arg1	importance					2507:2516	the functional importance	2492:2516	the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development	2492:2622	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	11	3	theme	reduced	2158:2164	arg1	set					2171:2173	the reduced seed set	2154:2173	the reduced seed set in these mutants	2154:2190	Pollen defects likely contributed to the reduced seed set in these mutants.					
34903166	8	4	from	impairment	1640:1649	arg1	mutants					1669:1675	these Hyp-GALT mutants	1654:1675	these Hyp-GALT mutants	1654:1675	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	7	5	theme	mutants	1504:1510	arg1	AGPs					1483:1486	the silique and root AGPs	1462:1486	AGPs	1483:1486	Monosaccharide composition analysis showed that galactose was decreased in the silique and root AGPs of the Hyp-GALT mutants.					
34903166	10	6	theme	salt	1998:2001	arg1	stress					2003:2008	salt stress	1998:2008	salt stress	1998:2008	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	2	7	theme	II	370:371	arg1	arabinogalactans					373:388	type II arabinogalactans	365:388	type II arabinogalactans	365:388	AGPs biosynthesis involves a series of post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues.					
34903166	11	8	from	set	2171:2173	arg1	mutants					2184:2190	these mutants	2178:2190	these mutants	2178:2190	Pollen defects likely contributed to the reduced seed set in these mutants.					
34903166	9	9	theme	equal	1761:1765	arg1	effects					1776:1782	equal additive effects	1761:1782	equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability	1761:1888	Correlated with expression patterns, galt2, galt5, galt7, galt8, and galt9 display equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability.					
34903166	5	10	theme	gene	769:772	arg1	redundancy					774:783	their gene redundancy	763:783	their gene redundancy	763:783	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	13	11	theme	plant	2595:2599	arg1	aspects					2584:2590	various aspects	2576:2590	various aspects of plant growth and development	2576:2622	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	11	12	theme	Pollen	2117:2122	arg1	defects					2124:2130	Pollen defects	2117:2130	Pollen defects	2117:2130	Pollen defects likely contributed to the reduced seed set in these mutants.					
34903166	6	13	theme	AGP	1250:1252	arg1	glycosylation					1254:1266	AGP glycosylation	1250:1266	AGP glycosylation in cauline and rosette leaves	1250:1296	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	4	14	theme	Hyp-GALT	722:729	arg1	family					736:741	the Hyp-GALT gene family	718:741	the Hyp-GALT gene family	718:741	The extent of genetic redundancy, however, remains to be elucidated for the Hyp-GALT gene family.					
34903166	2	15	theme	Hyp	408:410	arg1	residues					412:419	non-contiguous Hyp residues	393:419	non-contiguous Hyp residues	393:419	AGPs biosynthesis involves a series of post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues.					
34903166	6	16	with	precipitations	1133:1146	arg1	reagent					1161:1167	β-Yariv reagent	1153:1167	β-Yariv reagent	1153:1167	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	2	17	theme	modifications	325:337	arg1	series					296:301	a series	294:301	a series of post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues	294:419	AGPs biosynthesis involves a series of post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues.					
34903166	5	18	theme	quadruple	898:906	arg1	mutants					908:914	two quadruple mutants	894:914	two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9)	894:965	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	9	19	theme	β-Yariv-induced	1804:1818	arg1	inhibition					1827:1836	β-Yariv-induced growth inhibition	1804:1836	β-Yariv-induced growth inhibition	1804:1836	Correlated with expression patterns, galt2, galt5, galt7, galt8, and galt9 display equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability.					
34903166	8	20	theme	quintuple	1535:1543	arg1	stems					1552:1556	25789 quintuple mutant stems	1529:1556	25789 quintuple mutant stems	1529:1556	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	6	21	theme	GALT7	1321:1325	arg1	activity					1309:1316	the activity	1305:1316	the activity of GALT7, GALT8 and GALT9	1305:1342	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	10	22	theme	more	2042:2045	arg1	roles					2057:2061	more important roles	2042:2061	more important roles	2042:2061	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	1	23	gly	glycoproteins	220:232	arg1	Arabinogalactan-proteins					133:156	BACKGROUND Arabinogalactan-proteins	122:156	BACKGROUND Arabinogalactan-proteins (AGPs)	122:163	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	1	23	gly	glycoproteins	220:232	arg1	glycoproteins					220:232	structurally complex hydroxyproline-rich cell wall glycoproteins	169:232	structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom	169:264	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	1	24	theme	hydroxyproline-rich	190:208	arg1	Arabinogalactan-proteins					133:156	BACKGROUND Arabinogalactan-proteins	122:156	BACKGROUND Arabinogalactan-proteins (AGPs)	122:163	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	1	24	theme	hydroxyproline-rich	190:208	arg1	glycoproteins					220:232	structurally complex hydroxyproline-rich cell wall glycoproteins	169:232	structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom	169:264	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	8	25	theme	stems	1552:1556	arg1	analysis					1517:1524	TEM analysis	1513:1524	TEM analysis of 25789 quintuple mutant stems	1513:1556	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	13	26	theme	mutants	2472:2478	arg1	generation					2436:2445	the generation	2432:2445	the generation of higher order Hyp-GALT mutants	2432:2478	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	1	27	theme	wall	215:218	arg1	Arabinogalactan-proteins					133:156	BACKGROUND Arabinogalactan-proteins	122:156	BACKGROUND Arabinogalactan-proteins (AGPs)	122:163	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	1	27	theme	wall	215:218	arg1	glycoproteins					220:232	structurally complex hydroxyproline-rich cell wall glycoproteins	169:232	structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom	169:264	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	9	28	theme	silique	1839:1845	arg1	length					1847:1852	silique length	1839:1852	silique length	1839:1852	Correlated with expression patterns, galt2, galt5, galt7, galt8, and galt9 display equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability.					
34903166	8	29	theme	cell	1568:1571	arg1	defects					1578:1584	cell wall defects	1568:1584	cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants	1568:1675	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	10	30	theme	seed	2066:2069	arg1	morphology					2071:2080	seed morphology	2066:2080	seed morphology	2066:2080	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	1	31	theme	ubiquitous	234:243	arg1	Arabinogalactan-proteins					133:156	BACKGROUND Arabinogalactan-proteins	122:156	BACKGROUND Arabinogalactan-proteins (AGPs)	122:163	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	1	31	theme	ubiquitous	234:243	arg1	glycoproteins					220:232	structurally complex hydroxyproline-rich cell wall glycoproteins	169:232	structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom	169:264	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	6	32	theme	AGP	1129:1131	arg1	precipitations					1133:1146	AGP precipitations	1129:1146	AGP precipitations with β-Yariv reagent	1129:1167	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	4	33	theme	redundancy	668:677	arg1	extent					650:655	The extent	646:655	The extent of genetic redundancy	646:677	The extent of genetic redundancy, however, remains to be elucidated for the Hyp-GALT gene family.					
34903166	10	34	theme	seed	2107:2110	arg1	set					2112:2114	seed set	2107:2114	seed set	2107:2114	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	5	35	theme	triple	859:864	arg1	galt5 galt8 galt9					874:890	galt5 galt8 galt9	874:890	galt5 galt8 galt9	874:890	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	35	theme	triple	859:864	arg1	mutant					866:871	a triple mutant	857:871	a triple mutant (galt5 galt8 galt9)	857:891	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	12	36	theme	combinations	2372:2383	arg1	generation					2348:2357	generation	2348:2357	generation of different combinations of Hyp-GALT knock-out mutants	2348:2413	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	12	37	theme	GALT9	2271:2275	arg1	effects					2229:2235	CONCLUSION Additive and pleiotropic effects	2193:2235	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes	2193:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	5	38	theme	multiple	821:828	arg1	mutant					866:871	a triple mutant	857:871	a triple mutant (galt5 galt8 galt9)	857:891	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	38	theme	multiple	821:828	arg1	knock-outs					835:844	various multiple gene knock-outs	813:844	various multiple gene knock-outs	813:844	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	38	theme	multiple	821:828	arg1	mutant					986:991	one quintuple mutant	972:991	one quintuple mutant (galt2 galt5 galt7 galt8 galt9)	972:1023	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	38	theme	multiple	821:828	arg1	mutants					908:914	two quadruple mutants	894:914	two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9)	894:965	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	1	39	theme	BACKGROUND	122:131	arg1	Arabinogalactan-proteins					133:156	BACKGROUND Arabinogalactan-proteins	122:156	BACKGROUND Arabinogalactan-proteins (AGPs)	122:163	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	1	39	theme	BACKGROUND	122:131	arg1	glycoproteins					220:232	structurally complex hydroxyproline-rich cell wall glycoproteins	169:232	structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom	169:264	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	1	39	theme	BACKGROUND	122:131	arg1	AGPs					159:162	AGPs	159:162	AGPs	159:162	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	6	40	theme	key	1107:1109	arg1	findings					1111:1118	The key findings	1103:1118	The key findings	1103:1118	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	8	41	theme	observed	1606:1613	arg1	impairment					1640:1649	the observed developmental and growth impairment	1602:1649	the observed developmental and growth impairment in these Hyp-GALT mutants	1602:1675	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	12	42	theme	vegetative	2280:2289	arg1	phenotypes					2315:2324	vegetative and reproductive growth phenotypes	2280:2324	vegetative and reproductive growth phenotypes	2280:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	12	43	theme	growth	2308:2313	arg1	phenotypes					2315:2324	vegetative and reproductive growth phenotypes	2280:2324	vegetative and reproductive growth phenotypes	2280:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	12	44	theme	GALT7	2254:2258	arg1	effects					2229:2235	CONCLUSION Additive and pleiotropic effects	2193:2235	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes	2193:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	8	45	with	coincident	1586:1595	arg1	impairment					1640:1649	the observed developmental and growth impairment	1602:1649	the observed developmental and growth impairment in these Hyp-GALT mutants	1602:1675	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	0	46	theme	Functional	0:9	arg1	characterization					11:26	Functional characterization	0:26	Functional characterization of hydroxyproline-O-galactosyltransferases for Arabidopsis arabinogalactan-protein synthesis.	0:120	Functional characterization of hydroxyproline-O-galactosyltransferases for Arabidopsis arabinogalactan-protein synthesis.					
34903166	12	47	theme	different	2362:2370	arg1	combinations					2372:2383	different combinations	2362:2383	different combinations of Hyp-GALT knock-out mutants	2362:2413	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	13	48	theme	Hyp-GALT	2463:2470	arg1	mutants					2472:2478	higher order Hyp-GALT mutants	2450:2478	higher order Hyp-GALT mutants	2450:2478	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	12	49	theme	GALT8	2261:2265	arg1	effects					2229:2235	CONCLUSION Additive and pleiotropic effects	2193:2235	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes	2193:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	3	50	theme	first	555:559	arg1	residues					571:578	the first galactose residues	551:578	the first galactose residues	551:578	To date, eight Hyp-galactosyltransferases (Hyp-GALTs; GALT2-GALT9) belonging to CAZy GT31, are known to catalyze the addition of the first galactose residues to AGP protein backbones and enable subsequent AGP glycosylation.					
34903166	3	51	theme	AGP	627:629	arg1	glycosylation					631:643	subsequent AGP glycosylation	616:643	subsequent AGP glycosylation	616:643	To date, eight Hyp-galactosyltransferases (Hyp-GALTs; GALT2-GALT9) belonging to CAZy GT31, are known to catalyze the addition of the first galactose residues to AGP protein backbones and enable subsequent AGP glycosylation.					
34903166	7	52	theme	Monosaccharide	1387:1400	arg1	analysis					1414:1421	Monosaccharide composition analysis	1387:1421	Monosaccharide composition analysis	1387:1421	Monosaccharide composition analysis showed that galactose was decreased in the silique and root AGPs of the Hyp-GALT mutants.					
34903166	4	53	theme	genetic	660:666	arg1	redundancy					668:677	genetic redundancy	660:677	genetic redundancy	660:677	The extent of genetic redundancy, however, remains to be elucidated for the Hyp-GALT gene family.					
34903166	12	54	theme	GALT2	2240:2244	arg1	effects					2229:2235	CONCLUSION Additive and pleiotropic effects	2193:2235	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes	2193:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	6	55	from	glycosylation	1254:1266	arg1	rosette					1283:1289	rosette leaves	1283:1296	rosette leaves	1283:1296	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	6	55	from	glycosylation	1254:1266	arg1	cauline					1271:1277	cauline	1271:1277	cauline	1271:1277	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	12	56	theme	GALT5	2247:2251	arg1	effects					2229:2235	CONCLUSION Additive and pleiotropic effects	2193:2235	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes	2193:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	3	57	theme	AGP	583:585	arg1	backbones					595:603	AGP protein backbones	583:603	AGP protein backbones	583:603	To date, eight Hyp-galactosyltransferases (Hyp-GALTs; GALT2-GALT9) belonging to CAZy GT31, are known to catalyze the addition of the first galactose residues to AGP protein backbones and enable subsequent AGP glycosylation.					
34903166	0	58	theme	arabinogalactan-protein	87:109	arg1	synthesis					111:119	Arabidopsis arabinogalactan-protein synthesis	75:119	Arabidopsis arabinogalactan-protein synthesis	75:119	Functional characterization of hydroxyproline-O-galactosyltransferases for Arabidopsis arabinogalactan-protein synthesis.					
34903166	13	59	theme	higher	2450:2455	arg1	mutants					2472:2478	higher order Hyp-GALT mutants	2450:2478	higher order Hyp-GALT mutants	2450:2478	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	10	60	theme	root	1958:1961	arg1	growth					1963:1968	root growth	1958:1968	root growth	1958:1968	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	3	61	theme	residues	571:578	arg1	addition					539:546	the addition	535:546	the addition of the first galactose residues to AGP protein backbones	535:603	To date, eight Hyp-galactosyltransferases (Hyp-GALTs; GALT2-GALT9) belonging to CAZy GT31, are known to catalyze the addition of the first galactose residues to AGP protein backbones and enable subsequent AGP glycosylation.					
34903166	10	62	theme	tip	1979:1981	arg1	swelling					1983:1990	primary root growth and root tip swelling	1950:1990	primary root growth and root tip swelling under salt stress	1950:2008	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	5	63	dep	mutants	908:914	arg1	galt2 galt5 galt7 galt9					942:964	galt2 galt5 galt7 galt9	942:964	galt2 galt5 galt7 galt9	942:964	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	63	dep	mutants	908:914	arg1	galt2 galt5 galt7 galt8					917:939	galt2 galt5 galt7 galt8	917:939	galt2 galt5 galt7 galt8	917:939	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	2	64	theme	non-contiguous	393:406	arg1	residues					412:419	non-contiguous Hyp residues	393:419	non-contiguous Hyp residues	393:419	AGPs biosynthesis involves a series of post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues.					
34903166	11	65	theme	seed	2166:2169	arg1	set					2171:2173	the reduced seed set	2154:2173	the reduced seed set in these mutants	2154:2190	Pollen defects likely contributed to the reduced seed set in these mutants.					
34903166	13	66	theme	various	2576:2582	arg1	aspects					2584:2590	various aspects	2576:2590	various aspects of plant growth and development	2576:2622	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	2	67	theme	arabinogalactans	373:388	arg1	addition					353:360	the addition	349:360	the addition of type II arabinogalactans to non-contiguous Hyp residues	349:419	AGPs biosynthesis involves a series of post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues.					
34903166	1	68	theme	plant	252:256	arg1	kingdom					258:264	the plant kingdom	248:264	the plant kingdom	248:264	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	6	69	gly	glycosylation	1254:1266	arg1	rosette					1283:1289	rosette leaves	1283:1296	rosette leaves	1283:1296	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	6	69	gly	glycosylation	1254:1266	arg1	cauline					1271:1277	cauline	1271:1277	cauline	1271:1277	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	6	70	theme	GALT9	1338:1342	arg1	activity					1309:1316	the activity	1305:1316	the activity of GALT7, GALT8 and GALT9	1305:1342	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	9	71	theme	additive	1767:1774	arg1	effects					1776:1782	equal additive effects	1761:1782	equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability	1761:1888	Correlated with expression patterns, galt2, galt5, galt7, galt8, and galt9 display equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability.					
34903166	7	72	theme	Hyp-GALT	1495:1502	arg1	mutants					1504:1510	the Hyp-GALT mutants	1491:1510	the Hyp-GALT mutants	1491:1510	Monosaccharide composition analysis showed that galactose was decreased in the silique and root AGPs of the Hyp-GALT mutants.					
34903166	12	73	theme	Hyp-GALT	2388:2395	arg1	mutants					2407:2413	Hyp-GALT knock-out mutants	2388:2413	Hyp-GALT knock-out mutants	2388:2413	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	8	74	theme	mutant	1545:1550	arg1	stems					1552:1556	25789 quintuple mutant stems	1529:1556	25789 quintuple mutant stems	1529:1556	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	7	75	theme	root	1478:1481	arg1	AGPs					1483:1486	the silique and root AGPs	1462:1486	AGPs	1483:1486	Monosaccharide composition analysis showed that galactose was decreased in the silique and root AGPs of the Hyp-GALT mutants.					
34903166	4	76	theme	gene	731:734	arg1	family					736:741	the Hyp-GALT gene family	718:741	the Hyp-GALT gene family	718:741	The extent of genetic redundancy, however, remains to be elucidated for the Hyp-GALT gene family.					
34903166	10	77	theme	important	2047:2055	arg1	roles					2057:2061	more important roles	2042:2061	more important roles	2042:2061	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	13	78	theme	AG	2521:2522	arg1	polysaccharides					2524:2538	AG polysaccharides	2521:2538	AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development	2521:2622	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	2	79	theme	post-translational	306:323	arg1	modifications					325:337	post-translational modifications	306:337	post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues	306:419	AGPs biosynthesis involves a series of post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues.					
34903166	2	79	theme	post-translational	306:323	arg1	addition					353:360	the addition	349:360	the addition of type II arabinogalactans to non-contiguous Hyp residues	349:419	AGPs biosynthesis involves a series of post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues.					
34903166	1	80	theme	complex	182:188	arg1	Arabinogalactan-proteins					133:156	BACKGROUND Arabinogalactan-proteins	122:156	BACKGROUND Arabinogalactan-proteins (AGPs)	122:163	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	1	80	theme	complex	182:188	arg1	glycoproteins					220:232	structurally complex hydroxyproline-rich cell wall glycoproteins	169:232	structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom	169:264	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	9	81	theme	growth	1820:1825	arg1	inhibition					1827:1836	β-Yariv-induced growth inhibition	1804:1836	β-Yariv-induced growth inhibition	1804:1836	Correlated with expression patterns, galt2, galt5, galt7, galt8, and galt9 display equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability.					
34903166	7	82	theme	silique	1466:1472	arg1	AGPs					1483:1486	the silique and root AGPs	1462:1486	AGPs	1483:1486	Monosaccharide composition analysis showed that galactose was decreased in the silique and root AGPs of the Hyp-GALT mutants.					
34903166	2	83	theme	type	365:368	arg1	arabinogalactans					373:388	type II arabinogalactans	365:388	type II arabinogalactans	365:388	AGPs biosynthesis involves a series of post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues.					
34903166	1	84	theme	cell	210:213	arg1	Arabinogalactan-proteins					133:156	BACKGROUND Arabinogalactan-proteins	122:156	BACKGROUND Arabinogalactan-proteins (AGPs)	122:163	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	1	84	theme	cell	210:213	arg1	glycoproteins					220:232	structurally complex hydroxyproline-rich cell wall glycoproteins	169:232	structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom	169:264	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	6	85	theme	β-Yariv	1153:1159	arg1	reagent					1161:1167	β-Yariv reagent	1153:1167	β-Yariv reagent	1153:1167	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	8	86	theme	wall	1573:1576	arg1	defects					1578:1584	cell wall defects	1568:1584	cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants	1568:1675	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	5	87	theme	quintuple	976:984	arg1	galt2 galt5 galt7 galt8 galt9					994:1022	galt2 galt5 galt7 galt8 galt9	994:1022	galt2 galt5 galt7 galt8 galt9	994:1022	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	87	theme	quintuple	976:984	arg1	mutant					986:991	one quintuple mutant	972:991	one quintuple mutant (galt2 galt5 galt7 galt8 galt9)	972:1023	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	8	88	theme	coincident	1586:1595	arg1	defects					1578:1584	cell wall defects	1568:1584	cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants	1568:1675	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	1	89	from	kingdom	258:264	arg1	ubiquitous					234:243	ubiquitous	234:243	ubiquitous	234:243	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	12	90	theme	reproductive	2295:2306	arg1	phenotypes					2315:2324	vegetative and reproductive growth phenotypes	2280:2324	vegetative and reproductive growth phenotypes	2280:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	9	91	theme	plant	1855:1859	arg1	height					1861:1866	plant height	1855:1866	plant height	1855:1866	Correlated with expression patterns, galt2, galt5, galt7, galt8, and galt9 display equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability.					
34903166	5	92	theme	various	813:819	arg1	mutant					866:871	a triple mutant	857:871	a triple mutant (galt5 galt8 galt9)	857:891	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	92	theme	various	813:819	arg1	knock-outs					835:844	various multiple gene knock-outs	813:844	various multiple gene knock-outs	813:844	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	92	theme	various	813:819	arg1	mutant					986:991	one quintuple mutant	972:991	one quintuple mutant (galt2 galt5 galt7 galt8 galt9)	972:1023	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	92	theme	various	813:819	arg1	mutants					908:914	two quadruple mutants	894:914	two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9)	894:965	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	93	theme	physiological	1077:1089	arg1	phenotypes					1091:1100	their biochemical and physiological phenotypes	1055:1100	their biochemical and physiological phenotypes	1055:1100	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	9	94	from	effects	1776:1782	arg1	insensitivity					1787:1799	insensitivity	1787:1799	insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability	1787:1888	Correlated with expression patterns, galt2, galt5, galt7, galt8, and galt9 display equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability.					
34903166	8	95	theme	developmental	1615:1627	arg1	impairment					1640:1649	the observed developmental and growth impairment	1602:1649	the observed developmental and growth impairment in these Hyp-GALT mutants	1602:1675	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	13	96	theme	functional	2496:2505	arg1	importance					2507:2516	the functional importance	2492:2516	the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development	2492:2622	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	10	97	theme	germination	2083:2093	arg1	defects					2095:2101	germination defects	2083:2101	germination defects	2083:2101	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	5	98	theme	gene	830:833	arg1	mutant					866:871	a triple mutant	857:871	a triple mutant (galt5 galt8 galt9)	857:891	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	98	theme	gene	830:833	arg1	knock-outs					835:844	various multiple gene knock-outs	813:844	various multiple gene knock-outs	813:844	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	98	theme	gene	830:833	arg1	mutant					986:991	one quintuple mutant	972:991	one quintuple mutant (galt2 galt5 galt7 galt8 galt9)	972:1023	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	5	98	theme	gene	830:833	arg1	mutants					908:914	two quadruple mutants	894:914	two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9)	894:965	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	1	99	from	ubiquitous	234:243	arg1	kingdom					258:264	the plant kingdom	248:264	the plant kingdom	248:264	BACKGROUND Arabinogalactan-proteins (AGPs) are structurally complex hydroxyproline-rich cell wall glycoproteins ubiquitous in the plant kingdom.					
34903166	9	100	theme	pollen	1873:1878	arg1	viability					1880:1888	pollen viability	1873:1888	pollen viability	1873:1888	Correlated with expression patterns, galt2, galt5, galt7, galt8, and galt9 display equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability.					
34903166	8	101	theme	growth	1633:1638	arg1	impairment					1640:1649	the observed developmental and growth impairment	1602:1649	the observed developmental and growth impairment in these Hyp-GALT mutants	1602:1675	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	13	102	theme	order	2457:2461	arg1	mutants					2472:2478	higher order Hyp-GALT mutants	2450:2478	higher order Hyp-GALT mutants	2450:2478	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	7	103	theme	composition	1402:1412	arg1	analysis					1414:1421	Monosaccharide composition analysis	1387:1421	Monosaccharide composition analysis	1387:1421	Monosaccharide composition analysis showed that galactose was decreased in the silique and root AGPs of the Hyp-GALT mutants.					
34903166	0	104	theme	hydroxyproline-O-galactosyltransferases	31:69	arg1	characterization					11:26	Functional characterization	0:26	Functional characterization of hydroxyproline-O-galactosyltransferases for Arabidopsis arabinogalactan-protein synthesis.	0:120	Functional characterization of hydroxyproline-O-galactosyltransferases for Arabidopsis arabinogalactan-protein synthesis.					
34903166	5	105	theme	biochemical	1061:1071	arg1	phenotypes					1091:1100	their biochemical and physiological phenotypes	1055:1100	their biochemical and physiological phenotypes	1055:1100	RESULTS To examine their gene redundancy and functions, we generated various multiple gene knock-outs, including a triple mutant (galt5 galt8 galt9), two quadruple mutants (galt2 galt5 galt7 galt8, galt2 galt5 galt7 galt9), and one quintuple mutant (galt2 galt5 galt7 galt8 galt9), and comprehensively examined their biochemical and physiological phenotypes.					
34903166	6	106	theme	GALT8	1328:1332	arg1	activity					1309:1316	the activity	1305:1316	the activity of GALT7, GALT8 and GALT9	1305:1342	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	8	107	theme	Hyp-GALT	1660:1667	arg1	mutants					1669:1675	these Hyp-GALT mutants	1654:1675	these Hyp-GALT mutants	1654:1675	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	2	108	theme	AGPs	267:270	arg1	biosynthesis					272:283	AGPs biosynthesis	267:283	AGPs biosynthesis	267:283	AGPs biosynthesis involves a series of post-translational modifications including the addition of type II arabinogalactans to non-contiguous Hyp residues.					
34903166	3	109	theme	galactose	561:569	arg1	residues					571:578	the first galactose residues	551:578	the first galactose residues	551:578	To date, eight Hyp-galactosyltransferases (Hyp-GALTs; GALT2-GALT9) belonging to CAZy GT31, are known to catalyze the addition of the first galactose residues to AGP protein backbones and enable subsequent AGP glycosylation.					
34903166	0	110	theme	Arabidopsis	75:85	arg1	synthesis					111:119	Arabidopsis arabinogalactan-protein synthesis	75:119	Arabidopsis arabinogalactan-protein synthesis	75:119	Functional characterization of hydroxyproline-O-galactosyltransferases for Arabidopsis arabinogalactan-protein synthesis.					
34903166	9	111	theme	expression	1694:1703	arg1	patterns					1705:1712	expression patterns	1694:1712	expression patterns	1694:1712	Correlated with expression patterns, galt2, galt5, galt7, galt8, and galt9 display equal additive effects on insensitivity to β-Yariv-induced growth inhibition, silique length, plant height, and pollen viability.					
34903166	13	112	dep	plant	2595:2599	arg1	development					2612:2622	development	2612:2622	development	2612:2622	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	13	112	dep	plant	2595:2599	arg1	growth					2601:2606	growth	2601:2606	growth	2601:2606	Taken together, the generation of higher order Hyp-GALT mutants demonstrate the functional importance of AG polysaccharides decorating the AGPs with respect to various aspects of plant growth and development.					
34903166	3	113	dep	Hyp-GALTs	465:473	arg1	GALT2-GALT9					476:486	GALT2-GALT9	476:486	Hyp-GALTs; GALT2-GALT9	465:486	To date, eight Hyp-galactosyltransferases (Hyp-GALTs; GALT2-GALT9) belonging to CAZy GT31, are known to catalyze the addition of the first galactose residues to AGP protein backbones and enable subsequent AGP glycosylation.					
34903166	10	114	theme	growth	1963:1968	arg1	swelling					1983:1990	primary root growth and root tip swelling	1950:1990	primary root growth and root tip swelling under salt stress	1950:2008	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	12	115	theme	mutants	2407:2413	arg1	combinations					2372:2383	different combinations	2362:2383	different combinations of Hyp-GALT knock-out mutants	2362:2413	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	3	116	theme	protein	587:593	arg1	backbones					595:603	AGP protein backbones	583:603	AGP protein backbones	583:603	To date, eight Hyp-galactosyltransferases (Hyp-GALTs; GALT2-GALT9) belonging to CAZy GT31, are known to catalyze the addition of the first galactose residues to AGP protein backbones and enable subsequent AGP glycosylation.					
34903166	12	117	theme	pleiotropic	2217:2227	arg1	effects					2229:2235	CONCLUSION Additive and pleiotropic effects	2193:2235	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes	2193:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	10	118	theme	primary	1950:1956	arg1	swelling					1983:1990	primary root growth and root tip swelling	1950:1990	primary root growth and root tip swelling under salt stress	1950:2008	Interestingly, galt7, galt8, and galt9 contributed more to primary root growth and root tip swelling under salt stress, whereas galt2 and galt5 played more important roles in seed morphology, germination defects and seed set.					
34903166	12	119	theme	Additive	2204:2211	arg1	effects					2229:2235	CONCLUSION Additive and pleiotropic effects	2193:2235	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes	2193:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
34903166	6	120	dep	rosette	1283:1289	arg1	leaves					1291:1296	leaves	1291:1296	leaves	1291:1296	The key findings include: AGP precipitations with β-Yariv reagent showed that GALT2, GALT5, GALT7, GALT8 and GALT9 act redundantly with respect to AGP glycosylation in cauline and rosette leaves, while the activity of GALT7, GALT8 and GALT9 dominate in the stem, silique and flowers.					
34903166	8	121	theme	TEM	1513:1515	arg1	analysis					1517:1524	TEM analysis	1513:1524	TEM analysis of 25789 quintuple mutant stems	1513:1556	TEM analysis of 25789 quintuple mutant stems indicated cell wall defects coincident with the observed developmental and growth impairment in these Hyp-GALT mutants.					
34903166	3	122	theme	subsequent	616:625	arg1	glycosylation					631:643	subsequent AGP glycosylation	616:643	subsequent AGP glycosylation	616:643	To date, eight Hyp-galactosyltransferases (Hyp-GALTs; GALT2-GALT9) belonging to CAZy GT31, are known to catalyze the addition of the first galactose residues to AGP protein backbones and enable subsequent AGP glycosylation.					
34903166	12	123	from	effects	2229:2235	arg1	phenotypes					2315:2324	vegetative and reproductive growth phenotypes	2280:2324	vegetative and reproductive growth phenotypes	2280:2324	CONCLUSION Additive and pleiotropic effects of GALT2, GALT5, GALT7, GALT8 and GALT9 on vegetative and reproductive growth phenotypes were teased apart via generation of different combinations of Hyp-GALT knock-out mutants.					
33992099	8	0	theme	samples	1046:1052	arg1	analysis					1004:1011	Histochemical and immunohistochemical analysis	966:1011	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples	966:1052	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	4	1	theme	infertile	639:647	arg1	women					649:653	infertile women	639:653	infertile women	639:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	14	2	theme	thin	2320:2323	arg1	endometrium					2325:2335	thin endometrium	2320:2335	thin endometrium	2320:2335	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	4	3	from	surface	566:572	arg1	endometrium					624:634	thin endometrium	619:634	thin endometrium of infertile women	619:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	12	4	theme	glandular	2068:2076	arg1	cells					2089:2093	the luminal and glandular epithelial cells	2052:2093	the luminal and glandular epithelial cells	2052:2093	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	0	5	with	women	76:80	arg1	study					123:127	a pilot observational study	101:127	a pilot observational study	101:127	Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study.					
33992099	0	5	with	women	76:80	arg1	infertility					87:97	infertility	87:97	infertility	87:97	Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study.					
33992099	2	6	theme	thin	300:303	arg1	endometrium					305:315	thin endometrium	300:315	thin endometrium	300:315	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	12	7	theme	cells	2089:2093	arg1	surfaces					2040:2047	the apical surfaces	2029:2047	the apical surfaces of the luminal and glandular epithelial cells	2029:2093	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	10	8	theme	unit	1625:1628	arg1	Galβ1-4GlcNAcβ					1630:1643	a type-2 unit Galβ1-4GlcNAcβ	1616:1643	a type-2 unit Galβ1-4GlcNAcβ (LacNAc)	1616:1652	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	10	8	theme	unit	1625:1628	arg1	LacNAc					1646:1651	LacNAc	1646:1651	LacNAc	1646:1651	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	12	9	with	patients	2108:2115	arg1	endometrium					2127:2137	thin endometrium	2122:2137	thin endometrium	2122:2137	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	12	9	with	patients	2108:2115	arg1	failure					2166:2172	recurrent implantation failure	2143:2172	recurrent implantation failure	2143:2172	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	14	10	theme	glycans	2271:2277	arg1	features					2255:2262	Specific features	2246:2262	Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity	2246:2438	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	5	11	theme	in	729:730	arg1	fertilization					738:750	in vitro fertilization	729:750	in vitro fertilization (IVF)	729:756	METHODS Thirty-two patients with tubal-peritoneal infertility undergoing in vitro fertilization (IVF) were included in the study.					
33992099	5	11	theme	in	729:730	arg1	IVF					753:755	IVF	753:755	IVF	753:755	METHODS Thirty-two patients with tubal-peritoneal infertility undergoing in vitro fertilization (IVF) were included in the study.					
33992099	7	12	theme	normal	903:908	arg1	≥8 mm					923:927	≥8 mm	923:927	≥8 mm	923:927	Patients were divided into two groups: patients with normal endometrium (≥8 mm) and with thin endometrium (< 8 mm).					
33992099	7	12	theme	normal	903:908	arg1	endometrium					910:920	normal endometrium	903:920	normal endometrium (≥8 mm)	903:928	Patients were divided into two groups: patients with normal endometrium (≥8 mm) and with thin endometrium (< 8 mm).					
33992099	1	13	theme	factors	189:195	arg1	factors					189:195	the major factors	179:195	the major factors affecting successful implantation in assisted reproductive technologies (ART)	179:273	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	1	13	theme	factors	189:195	arg1	one					172:174	one	172:174	one	172:174	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	12	14	theme	luminal	2056:2062	arg1	cells					2089:2093	the luminal and glandular epithelial cells	2052:2093	the luminal and glandular epithelial cells	2052:2093	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	11	15	from	Expression	1813:1822	arg1	surfaces					1869:1876	the apical surfaces	1858:1876	the apical surfaces of the luminal and glandular epithelial cells	1858:1922	Expression of the lectins-stained glycans on the apical surfaces of the luminal and glandular epithelial cells did not differ significantly.					
33992099	11	16	theme	glycans	1847:1853	arg1	Expression					1813:1822	Expression	1813:1822	Expression of the lectins-stained glycans on the apical surfaces of the luminal and glandular epithelial cells	1813:1922	Expression of the lectins-stained glycans on the apical surfaces of the luminal and glandular epithelial cells did not differ significantly.					
33992099	9	17	theme	glycan-binding	1273:1286	arg1	MAbs					1288:1291	glycan-binding MAbs	1273:1291	glycan-binding MAbs	1273:1291	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	1	18	theme	successful	207:216	arg1	implantation					218:229	successful implantation	207:229	successful implantation in assisted reproductive technologies (ART)	207:273	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	14	19	theme	Specific	2246:2253	arg1	features					2255:2262	Specific features	2246:2262	Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity	2246:2438	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	13	20	theme	mannose-rich	2212:2223	arg1	glycans					2225:2231	mannose-rich glycans	2212:2231	mannose-rich glycans	2212:2231	A similar relationship was shown for mannose-rich glycans.					
33992099	1	21	from	implantation	218:229	arg1	technologies					256:267	assisted reproductive technologies	234:267	assisted reproductive technologies (ART)	234:273	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	1	21	from	implantation	218:229	arg1	ART					270:272	ART	270:272	ART	270:272	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	12	22	theme	apical	2033:2038	arg1	surfaces					2040:2047	the apical surfaces	2029:2047	the apical surfaces of the luminal and glandular epithelial cells	2029:2093	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	10	23	theme	potential	1767:1775	arg1	they					1741:1744	they	1741:1744	they	1741:1744	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	10	23	theme	potential	1767:1775	arg1	markers					1777:1783	potential markers	1767:1783	potential markers of endometrium receptivity	1767:1810	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	12	24	theme	implantation	2153:2164	arg1	failure					2166:2172	recurrent implantation failure	2143:2172	recurrent implantation failure	2143:2172	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	10	25	dep	glycans	1597:1603	arg1	lacking					1658:1664	lacking	1658:1664	lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs	1658:1727	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	10	25	dep	glycans	1597:1603	arg1	containing					1605:1614	containing	1605:1614	containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc)	1605:1652	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	13	26	theme	similar	2177:2183	arg1	relationship					2185:2196	A similar relationship	2175:2196	A similar relationship	2175:2196	A similar relationship was shown for mannose-rich glycans.					
33992099	9	27	theme	L-selectin	1542:1551	arg1	glycans					1518:1524	MECA-79 glycans	1510:1524	MECA-79 glycans	1510:1524	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	9	27	theme	L-selectin	1542:1551	arg1	ligand					1532:1537	a ligand	1530:1537	a ligand of L-selectin	1530:1551	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	1	28	theme	Low	141:143	arg1	receptivity					157:167	BACKGROUND Low endometrial receptivity	130:167	BACKGROUND Low endometrial receptivity	130:167	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	2	29	with	patients	286:293	arg1	endometrium					305:315	thin endometrium	300:315	thin endometrium	300:315	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	12	30	theme	thin	2122:2125	arg1	endometrium					2127:2137	thin endometrium	2122:2137	thin endometrium	2122:2137	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	4	31	from	cells	610:614	arg1	endometrium					624:634	thin endometrium	619:634	thin endometrium of infertile women	619:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	12	32	from	expression	1978:1987	arg1	surfaces					2040:2047	the apical surfaces	2029:2047	the apical surfaces of the luminal and glandular epithelial cells	2029:2093	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	5	33	theme	METHODS	656:662	arg1	patients					675:682	METHODS Thirty-two patients	656:682	METHODS Thirty-two patients with tubal-peritoneal infertility undergoing in vitro fertilization (IVF)	656:756	METHODS Thirty-two patients with tubal-peritoneal infertility undergoing in vitro fertilization (IVF) were included in the study.					
33992099	4	34	theme	surface	566:572	arg1	glycocalyx					541:550	glycocalyx	541:550	glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women	541:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	9	35	theme	apical	1354:1359	arg1	surface					1361:1367	the apical surface	1350:1367	the apical surface of the luminal epithelial cells of thin endometrium	1350:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	10	36	theme	receptivity	1800:1810	arg1	they					1741:1744	they	1741:1744	they	1741:1744	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	10	36	theme	receptivity	1800:1810	arg1	markers					1777:1783	potential markers	1767:1783	potential markers of endometrium receptivity	1767:1810	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	14	37	theme	functional	2391:2400	arg1	layer					2402:2406	the endometrial functional layer	2375:2406	the endometrial functional layer	2375:2406	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	0	38	theme	pilot	103:107	arg1	study					123:127	a pilot observational study	101:127	a pilot observational study	101:127	Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study.					
33992099	3	39	theme	Molecular	418:426	arg1	pathophysiology					428:442	Molecular pathophysiology	418:442	Molecular pathophysiology of low receptivity of thin endometrium	418:481	Molecular pathophysiology of low receptivity of thin endometrium remains understudied.					
33992099	4	40	theme	glycocalyx	541:550	arg1	composition					526:536	composition	526:536	composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women	526:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	8	41	dep	lectins	1091:1097	arg1	MAL-II					1107:1112	MAL-II	1107:1112	MAL-II	1107:1112	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	8	41	dep	lectins	1091:1097	arg1	SNA					1115:1117	SNA	1115:1117	SNA	1115:1117	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	8	41	dep	lectins	1091:1097	arg1	A					1134:1134	Con A	1130:1134	Con A	1130:1134	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	8	41	dep	lectins	1091:1097	arg1	UEA-I					1100:1104	UEA-I	1100:1104	UEA-I	1100:1104	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	8	41	dep	lectins	1091:1097	arg1	VVL					1120:1122	VVL	1120:1122	VVL	1120:1122	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	8	41	dep	lectins	1091:1097	arg1	ECL					1125:1127	ECL	1125:1127	ECL	1125:1127	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	3	42	theme	thin	466:469	arg1	endometrium					471:481	thin endometrium	466:481	thin endometrium	466:481	Molecular pathophysiology of low receptivity of thin endometrium remains understudied.					
33992099	0	43	theme	Epithelial	0:9	arg1	changes					32:38	Epithelial apical glycosylation changes	0:38	Epithelial apical glycosylation changes	0:38	Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study.					
33992099	9	44	theme	luminal	1376:1382	arg1	cells					1395:1399	the luminal epithelial cells	1372:1399	the luminal epithelial cells of thin endometrium	1372:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	14	45	dep	features	2255:2262	arg1	essential					2344:2352	essential	2344:2352	essential	2344:2352	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	14	45	dep	features	2255:2262	arg1	explain					2412:2418	explain	2412:2418	explain its low receptivity	2412:2438	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	4	46	theme	cells	610:614	arg1	surface					566:572	the apical surface	555:572	the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women	555:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	0	47	theme	glycosylation	18:30	arg1	changes					32:38	Epithelial apical glycosylation changes	0:38	Epithelial apical glycosylation changes	0:38	Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study.					
33992099	9	48	theme	cells	1395:1399	arg1	surface					1361:1367	the apical surface	1350:1367	the apical surface of the luminal epithelial cells of thin endometrium	1350:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	8	49	theme	Con	1130:1132	arg1	UEA-I					1100:1104	UEA-I	1100:1104	UEA-I	1100:1104	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	8	49	theme	Con	1130:1132	arg1	A					1134:1134	Con A	1130:1134	Con A	1130:1134	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	7	50	with	patients	889:896	arg1	endometrium					944:954	thin endometrium	939:954	thin endometrium (< 8 mm)	939:963	Patients were divided into two groups: patients with normal endometrium (≥8 mm) and with thin endometrium (< 8 mm).					
33992099	7	50	with	patients	889:896	arg1	≥8 mm					923:927	≥8 mm	923:927	≥8 mm	923:927	Patients were divided into two groups: patients with normal endometrium (≥8 mm) and with thin endometrium (< 8 mm).					
33992099	7	50	with	patients	889:896	arg1	endometrium					910:920	normal endometrium	903:920	normal endometrium (≥8 mm)	903:928	Patients were divided into two groups: patients with normal endometrium (≥8 mm) and with thin endometrium (< 8 mm).					
33992099	7	50	with	patients	889:896	arg1	< 8 mm					957:962	< 8 mm	957:962	< 8 mm	957:962	Patients were divided into two groups: patients with normal endometrium (≥8 mm) and with thin endometrium (< 8 mm).					
33992099	8	51	theme	Histochemical	966:978	arg1	analysis					1004:1011	Histochemical and immunohistochemical analysis	966:1011	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples	966:1052	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	1	52	theme	endometrial	145:155	arg1	receptivity					157:167	BACKGROUND Low endometrial receptivity	130:167	BACKGROUND Low endometrial receptivity	130:167	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	3	53	theme	receptivity	451:461	arg1	pathophysiology					428:442	Molecular pathophysiology	418:442	Molecular pathophysiology of low receptivity of thin endometrium	418:481	Molecular pathophysiology of low receptivity of thin endometrium remains understudied.					
33992099	4	54	theme	thin	619:622	arg1	endometrium					624:634	thin endometrium	619:634	thin endometrium of infertile women	619:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	9	55	theme	thin	1404:1407	arg1	endometrium					1409:1419	thin endometrium	1404:1419	thin endometrium	1404:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	8	56	theme	immunohistochemical	984:1002	arg1	analysis					1004:1011	Histochemical and immunohistochemical analysis	966:1011	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples	966:1052	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	2	57	contain	have	317:320	arg1	patients					286:293	Infertile patients	276:293	Infertile patients with thin endometrium	276:315	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	2	57	contain	have	317:320	arg2	rate					374:377	a significantly lower cumulative clinical pregnancy rate	322:377	a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium	322:415	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	6	58	theme	menstrual	833:841	arg1	cycle					843:847	a natural menstrual cycle	823:847	a natural menstrual cycle	823:847	Endometrial samples were obtained in a natural menstrual cycle.					
33992099	11	59	theme	epithelial	1907:1916	arg1	cells					1918:1922	the luminal and glandular epithelial cells	1881:1922	the luminal and glandular epithelial cells	1881:1922	Expression of the lectins-stained glycans on the apical surfaces of the luminal and glandular epithelial cells did not differ significantly.					
33992099	0	60	theme	thin	56:59	arg1	endometrium					61:71	thin endometrium	56:71	thin endometrium in women with infertility - a pilot observational study	56:127	Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study.					
33992099	8	61	theme	anti-LeY	1141:1148	arg1	MAbs					1185:1188	MAbs	1185:1188	MAbs	1185:1188	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	8	61	theme	anti-LeY	1141:1148	arg1	antibodies					1173:1182	anti-LeY and MECA-79 monoclonal antibodies	1141:1182	antibodies	1173:1182	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	2	62	with	patients	384:391	arg1	endometrium					405:415	normal endometrium	398:415	normal endometrium	398:415	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	4	63	theme	glandular	589:597	arg1	cells					610:614	luminal and glandular epithelial cells	577:614	luminal and glandular epithelial cells in thin endometrium of infertile women	577:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	2	64	theme	clinical	355:362	arg1	rate					374:377	a significantly lower cumulative clinical pregnancy rate	322:377	a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium	322:415	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	9	65	theme	normal	1433:1438	arg1	endometrium					1440:1450	normal endometrium	1433:1450	normal endometrium	1433:1450	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	8	66	theme	MECA-79	1154:1160	arg1	MAbs					1185:1188	MAbs	1185:1188	MAbs	1185:1188	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	8	66	theme	MECA-79	1154:1160	arg1	antibodies					1173:1182	anti-LeY and MECA-79 monoclonal antibodies	1141:1182	antibodies	1173:1182	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	8	67	theme	endometrial	1034:1044	arg1	samples					1046:1052	paraffin-embedded endometrial samples	1016:1052	paraffin-embedded endometrial samples	1016:1052	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	10	68	theme	type-2	1618:1623	arg1	Galβ1-4GlcNAcβ					1630:1643	a type-2 unit Galβ1-4GlcNAcβ	1616:1643	a type-2 unit Galβ1-4GlcNAcβ (LacNAc)	1616:1652	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	10	68	theme	type-2	1618:1623	arg1	LacNAc					1646:1651	LacNAc	1646:1651	LacNAc	1646:1651	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	2	69	theme	normal	398:403	arg1	endometrium					405:415	normal endometrium	398:415	normal endometrium	398:415	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	4	70	theme	luminal	577:583	arg1	cells					610:614	luminal and glandular epithelial cells	577:614	luminal and glandular epithelial cells in thin endometrium of infertile women	577:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	14	71	theme	endometrium	2325:2335	arg1	compartments					2304:2315	epithelial compartments	2293:2315	epithelial compartments of thin endometrium	2293:2335	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	7	72	dep	groups	881:886	arg1	patients					889:896	patients	889:896	two groups: patients with normal endometrium (≥8 mm) and with thin endometrium (< 8 mm)	877:963	Patients were divided into two groups: patients with normal endometrium (≥8 mm) and with thin endometrium (< 8 mm).					
33992099	7	73	theme	thin	939:942	arg1	endometrium					944:954	thin endometrium	939:954	thin endometrium (< 8 mm)	939:963	Patients were divided into two groups: patients with normal endometrium (≥8 mm) and with thin endometrium (< 8 mm).					
33992099	7	73	theme	thin	939:942	arg1	< 8 mm					957:962	< 8 mm	957:962	< 8 mm	957:962	Patients were divided into two groups: patients with normal endometrium (≥8 mm) and with thin endometrium (< 8 mm).					
33992099	10	74	from	6-OH	1684:1687	arg1	binding					1705:1711	binding	1705:1711	binding to MECA-79 MAbs	1705:1727	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	10	74	from	6-OH	1684:1687	arg1	sulfo-residues					1666:1679	sulfo-residues	1666:1679	sulfo-residues at 6-OH of GlcNAcβ	1666:1698	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	8	75	theme	biotinylated	1078:1089	arg1	lectins					1091:1097	six biotinylated lectins	1074:1097	six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A)	1074:1135	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	4	76	theme	women	649:653	arg1	endometrium					624:634	thin endometrium	619:634	thin endometrium of infertile women	619:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	14	77	theme	epithelial	2293:2302	arg1	compartments					2304:2315	epithelial compartments	2293:2315	epithelial compartments of thin endometrium	2293:2335	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	5	78	theme	tubal-peritoneal	689:704	arg1	infertility					706:716	tubal-peritoneal infertility	689:716	tubal-peritoneal infertility undergoing in vitro fertilization (IVF)	689:756	METHODS Thirty-two patients with tubal-peritoneal infertility undergoing in vitro fertilization (IVF) were included in the study.					
33992099	10	79	theme	endometrium	1788:1798	arg1	receptivity					1800:1810	endometrium receptivity	1788:1810	endometrium receptivity	1788:1810	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	12	80	theme	epithelial	2078:2087	arg1	cells					2089:2093	the luminal and glandular epithelial cells	2052:2093	the luminal and glandular epithelial cells	2052:2093	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	9	81	theme	adjusted	1249:1256	arg1	specificity					1258:1268	the adjusted specificity	1245:1268	the adjusted specificity of glycan-binding MAbs	1245:1291	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	2	82	theme	lower	338:342	arg1	rate					374:377	a significantly lower cumulative clinical pregnancy rate	322:377	a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium	322:415	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	1	83	theme	major	183:187	arg1	factors					189:195	the major factors	179:195	the major factors affecting successful implantation in assisted reproductive technologies (ART)	179:273	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	14	84	theme	key	2267:2269	arg1	glycans					2271:2277	key glycans	2267:2277	key glycans	2267:2277	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	11	85	theme	apical	1862:1867	arg1	surfaces					1869:1876	the apical surfaces	1858:1876	the apical surfaces of the luminal and glandular epithelial cells	1858:1922	Expression of the lectins-stained glycans on the apical surfaces of the luminal and glandular epithelial cells did not differ significantly.					
33992099	9	86	from	surface	1361:1367	arg1	expression					1317:1326	1.3 times less expression	1302:1326	1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium	1302:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	14	87	from	expression	2279:2288	arg1	compartments					2304:2315	epithelial compartments	2293:2315	epithelial compartments of thin endometrium	2293:2335	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	10	88	theme	GlcNAcβ	1692:1698	arg1	6-OH					1684:1687	6-OH	1684:1687	6-OH of GlcNAcβ	1684:1698	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	11	89	theme	cells	1918:1922	arg1	surfaces					1869:1876	the apical surfaces	1858:1876	the apical surfaces of the luminal and glandular epithelial cells	1858:1922	Expression of the lectins-stained glycans on the apical surfaces of the luminal and glandular epithelial cells did not differ significantly.					
33992099	9	90	theme	MAbs	1288:1291	arg1	specificity					1258:1268	the adjusted specificity	1245:1268	the adjusted specificity of glycan-binding MAbs	1245:1291	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	12	91	theme	LeY	2022:2024	arg1	expression					1978:1987	the expression	1974:1987	the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells	1974:2093	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	5	92	with	patients	675:682	arg1	infertility					706:716	tubal-peritoneal infertility	689:716	tubal-peritoneal infertility undergoing in vitro fertilization (IVF)	689:756	METHODS Thirty-two patients with tubal-peritoneal infertility undergoing in vitro fertilization (IVF) were included in the study.					
33992099	11	93	theme	luminal	1885:1891	arg1	cells					1918:1922	the luminal and glandular epithelial cells	1881:1922	the luminal and glandular epithelial cells	1881:1922	Expression of the lectins-stained glycans on the apical surfaces of the luminal and glandular epithelial cells did not differ significantly.					
33992099	9	94	from	glycans	1339:1345	arg1	surface					1361:1367	the apical surface	1350:1367	the apical surface of the luminal epithelial cells of thin endometrium	1350:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	12	95	theme	recurrent	2143:2151	arg1	failure					2166:2172	recurrent implantation failure	2143:2172	recurrent implantation failure	2143:2172	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	9	96	theme	less	1312:1315	arg1	expression					1317:1326	1.3 times less expression	1302:1326	1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium	1302:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	2	97	theme	Infertile	276:284	arg1	patients					286:293	Infertile patients	276:293	Infertile patients with thin endometrium	276:315	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	1	98	theme	BACKGROUND	130:139	arg1	receptivity					157:167	BACKGROUND Low endometrial receptivity	130:167	BACKGROUND Low endometrial receptivity	130:167	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	12	99	gly	difucosylated	1992:2004	arg1	LeY					2022:2024	difucosylated oligosaccharide LeY	1992:2024	difucosylated oligosaccharide LeY	1992:2024	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	9	100	theme	intercellular	1565:1577	arg1	interactions					1579:1590	intercellular interactions	1565:1590	intercellular interactions	1565:1590	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	4	101	theme	apical	559:564	arg1	surface					566:572	the apical surface	555:572	the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women	555:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	1	102	theme	assisted	234:241	arg1	technologies					256:267	assisted reproductive technologies	234:267	assisted reproductive technologies (ART)	234:273	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	1	102	theme	assisted	234:241	arg1	ART					270:272	ART	270:272	ART	270:272	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	14	103	theme	low	2424:2426	arg1	receptivity					2428:2438	its low receptivity	2420:2438	its low receptivity	2420:2438	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	9	104	theme	glycans	1339:1345	arg1	expression					1317:1326	1.3 times less expression	1302:1326	1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium	1302:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	12	105	theme	difucosylated	1992:2004	arg1	LeY					2022:2024	difucosylated oligosaccharide LeY	1992:2024	difucosylated oligosaccharide LeY	1992:2024	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	11	106	theme	lectins-stained	1831:1845	arg1	glycans					1847:1853	the lectins-stained glycans	1827:1853	the lectins-stained glycans	1827:1853	Expression of the lectins-stained glycans on the apical surfaces of the luminal and glandular epithelial cells did not differ significantly.					
33992099	12	107	theme	oligosaccharide	2006:2020	arg1	LeY					2022:2024	difucosylated oligosaccharide LeY	1992:2024	difucosylated oligosaccharide LeY	1992:2024	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	5	108	theme	Thirty-two	664:673	arg1	patients					675:682	METHODS Thirty-two patients	656:682	METHODS Thirty-two patients with tubal-peritoneal infertility undergoing in vitro fertilization (IVF)	656:756	METHODS Thirty-two patients with tubal-peritoneal infertility undergoing in vitro fertilization (IVF) were included in the study.					
33992099	0	109	from	endometrium	61:71	arg1	women					76:80	women	76:80	women with infertility - a pilot observational study	76:127	Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study.					
33992099	12	110	located	found	2099:2103	arg2	Correlation					1954:1964	Correlation	1954:1964	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells	1954:2093	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	12	110	located	found	2099:2103	arg1	patients					2108:2115	patients	2108:2115	patients with thin endometrium and recurrent implantation failure	2108:2172	Correlation between the expression of difucosylated oligosaccharide LeY on the apical surfaces of the luminal and glandular epithelial cells was found in patients with thin endometrium and recurrent implantation failure.					
33992099	6	111	theme	Endometrial	786:796	arg1	samples					798:804	Endometrial samples	786:804	Endometrial samples	786:804	Endometrial samples were obtained in a natural menstrual cycle.					
33992099	11	112	theme	glandular	1897:1905	arg1	cells					1918:1922	the luminal and glandular epithelial cells	1881:1922	the luminal and glandular epithelial cells	1881:1922	Expression of the lectins-stained glycans on the apical surfaces of the luminal and glandular epithelial cells did not differ significantly.					
33992099	0	113	theme	observational	109:121	arg1	study					123:127	a pilot observational study	101:127	a pilot observational study	101:127	Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study.					
33992099	14	114	theme	layer	2402:2406	arg1	morphogenesis					2358:2370	morphogenesis	2358:2370	morphogenesis of the endometrial functional layer	2358:2406	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	14	115	theme	endometrial	2379:2389	arg1	layer					2402:2406	the endometrial functional layer	2375:2406	the endometrial functional layer	2375:2406	CONCLUSIONS Specific features of key glycans expression in epithelial compartments of thin endometrium may be essential for morphogenesis of the endometrial functional layer and explain its low receptivity.					
33992099	4	116	from	endometrium	624:634	arg1	surface					566:572	the apical surface	555:572	the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women	555:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	3	117	theme	endometrium	471:481	arg1	receptivity					451:461	low receptivity	447:461	low receptivity of thin endometrium	447:481	Molecular pathophysiology of low receptivity of thin endometrium remains understudied.					
33992099	9	118	theme	epithelial	1384:1393	arg1	cells					1395:1399	the luminal epithelial cells	1372:1399	the luminal epithelial cells of thin endometrium	1372:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	9	119	dep	RESULTS	1192:1198	arg1	analysis					1216:1223	analysis	1216:1223	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs	1192:1291	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	9	119	dep	RESULTS	1192:1198	arg1	glycans					1208:1214	Complex glycans	1200:1214	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs	1192:1291	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	5	120	dep	in	729:730	arg1	vitro					732:736	vitro	732:736	vitro	732:736	METHODS Thirty-two patients with tubal-peritoneal infertility undergoing in vitro fertilization (IVF) were included in the study.					
33992099	10	121	theme	MECA-79	1716:1722	arg1	MAbs					1724:1727	MECA-79 MAbs	1716:1727	MECA-79 MAbs	1716:1727	The glycans containing a type-2 unit Galβ1-4GlcNAcβ (LacNAc) but lacking sulfo-residues at 6-OH of GlcNAcβ, and binding to MECA-79 MAbs were found; they can be considered as potential markers of endometrium receptivity.					
33992099	9	122	theme	endometrium	1409:1419	arg1	cells					1395:1399	the luminal epithelial cells	1372:1399	the luminal epithelial cells of thin endometrium	1372:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	3	123	theme	low	447:449	arg1	receptivity					451:461	low receptivity	447:461	low receptivity of thin endometrium	447:481	Molecular pathophysiology of low receptivity of thin endometrium remains understudied.					
33992099	4	124	theme	epithelial	599:608	arg1	cells					610:614	luminal and glandular epithelial cells	577:614	luminal and glandular epithelial cells in thin endometrium of infertile women	577:653	We have investigated composition of glycocalyx of the apical surface of luminal and glandular epithelial cells in thin endometrium of infertile women.					
33992099	6	125	theme	natural	825:831	arg1	cycle					843:847	a natural menstrual cycle	823:847	a natural menstrual cycle	823:847	Endometrial samples were obtained in a natural menstrual cycle.					
33992099	2	126	theme	pregnancy	364:372	arg1	rate					374:377	a significantly lower cumulative clinical pregnancy rate	322:377	a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium	322:415	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	8	127	theme	monoclonal	1162:1171	arg1	MAbs					1185:1188	MAbs	1185:1188	MAbs	1185:1188	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	8	127	theme	monoclonal	1162:1171	arg1	antibodies					1173:1182	anti-LeY and MECA-79 monoclonal antibodies	1141:1182	antibodies	1173:1182	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	2	128	theme	cumulative	344:353	arg1	rate					374:377	a significantly lower cumulative clinical pregnancy rate	322:377	a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium	322:415	Infertile patients with thin endometrium have a significantly lower cumulative clinical pregnancy rate than patients with normal endometrium.					
33992099	1	129	theme	reproductive	243:254	arg1	technologies					256:267	assisted reproductive technologies	234:267	assisted reproductive technologies (ART)	234:273	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	1	129	theme	reproductive	243:254	arg1	ART					270:272	ART	270:272	ART	270:272	BACKGROUND Low endometrial receptivity is one of the major factors affecting successful implantation in assisted reproductive technologies (ART).					
33992099	9	130	from	expression	1317:1326	arg1	surface					1361:1367	the apical surface	1350:1367	the apical surface of the luminal epithelial cells of thin endometrium	1350:1419	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	8	131	theme	paraffin-embedded	1016:1032	arg1	samples					1046:1052	paraffin-embedded endometrial samples	1016:1052	paraffin-embedded endometrial samples	1016:1052	Histochemical and immunohistochemical analysis of paraffin-embedded endometrial samples was performed using six biotinylated lectins (UEA-I, MAL-II, SNA, VVL, ECL, Con A) and anti-LeY and MECA-79 monoclonal antibodies (MAbs).					
33992099	9	132	theme	Complex	1200:1206	arg1	glycans					1208:1214	Complex glycans	1200:1214	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs	1192:1291	RESULTS Complex glycans analysis taking into account the adjusted specificity of glycan-binding MAbs revealed 1.3 times less expression of MECA-79 glycans on the apical surface of the luminal epithelial cells of thin endometrium compared to normal endometrium; this deficiency may adversely affect implantation, since MECA-79 glycans are a ligand of L-selectin and mediate intercellular interactions.					
33992099	0	133	theme	apical	11:16	arg1	changes					32:38	Epithelial apical glycosylation changes	0:38	Epithelial apical glycosylation changes	0:38	Epithelial apical glycosylation changes associated with thin endometrium in women with infertility - a pilot observational study.					
32898301	5	0	theme	Protein	671:677	arg1	profiles					688:695	Protein N-glycan profiles	671:695	Protein N-glycan profiles	671:695	Protein N-glycan profiles were obtained using ultrahigh resolution mass spectrometry and included linkage-specific sialic acid information.					
32898301	6	1	theme	classification	865:878	arg1	performance					880:890	case-control classification performance	852:890	case-control classification performance	852:890	N-glycans were relatively quantified and case-control classification performance was evaluated based on glycosylation traits such as branching, fucosylation, and sialylation.					
32898301	10	2	from	classification	1604:1617	arg1	controls					1649:1656	healthy controls	1641:1656	healthy controls	1641:1656	CONCLUSION Serum N-glycome analysis revealed glycosylation differences that allow classification of PDAC patients from healthy controls.					
32898301	4	3	theme	independent	583:593	arg1	controls					619:626	26 cases/26 controls	607:626	26 cases/26 controls	607:626	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	4	3	theme	independent	583:593	arg1	validation					595:604	independent validation	583:604	independent validation (26 cases/26 controls)	583:627	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	10	4	theme	glycosylation	1567:1579	arg1	differences					1581:1591	glycosylation differences	1567:1591	glycosylation differences that allow classification of PDAC patients from healthy controls	1567:1656	CONCLUSION Serum N-glycome analysis revealed glycosylation differences that allow classification of PDAC patients from healthy controls.					
32898301	11	5	theme	glycosylation	1684:1696	arg1	basis					1801:1805	a basis	1799:1805	a basis for further development of a blood-based diagnostic test	1799:1862	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	11	5	theme	glycosylation	1684:1696	arg1	structures					1733:1742	single N-glycan structures	1717:1742	glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study	1684:1784	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	11	5	theme	glycosylation	1684:1696	arg1	traits					1698:1703	glycosylation traits	1684:1703	glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study	1684:1784	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	7	6	theme	N-glycans	1134:1142	arg1	branching					1029:1037	branching	1029:1037	branching (OR 6.19, P-value 9.21 × 10-11 )	1029:1070	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	7	6	theme	N-glycans	1134:1142	arg1	fucosylation					1085:1096	(antenna)fucosylation	1076:1096	(antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 )	1076:1129	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	4	7	theme	serum	654:658	arg1	specimens					660:668	in-house collected serum specimens	635:668	in-house collected serum specimens	635:668	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	9	8	theme	0.85	1444:1447	arg1	values					1434:1439	sensitivity and specificity values	1406:1439	sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set	1406:1519	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	8	9	theme	sialylation	1202:1212	arg1	higher					1218:1223	higher	1218:1223	higher	1218:1223	Furthermore, the ratio of α2,6- vs α2,3-linked sialylation was higher in patients compared to healthy controls.					
32898301	8	9	theme	sialylation	1202:1212	arg1	ratio					1172:1176	the ratio	1168:1176	the ratio of α2,6- vs α2,3-linked sialylation	1168:1212	Furthermore, the ratio of α2,6- vs α2,3-linked sialylation was higher in patients compared to healthy controls.					
32898301	9	10	theme	0.71	1453:1456	arg1	values					1434:1439	sensitivity and specificity values	1406:1439	sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set	1406:1519	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	2	11	theme	patient	305:311	arg1	survival					313:320	patient survival	305:320	patient survival	305:320	Novel diagnostic tools are needed to improve detection of PDAC and increase patient survival.					
32898301	4	12	theme	in-house	635:642	arg1	specimens					660:668	in-house collected serum specimens	635:668	in-house collected serum specimens	635:668	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	11	13	theme	diagnostic	1848:1857	arg1	test					1859:1862	a blood-based diagnostic test	1834:1862	a blood-based diagnostic test	1834:1862	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	5	14	theme	resolution	727:736	arg1	spectrometry					743:754	ultrahigh resolution mass spectrometry	717:754	ultrahigh resolution mass spectrometry	717:754	Protein N-glycan profiles were obtained using ultrahigh resolution mass spectrometry and included linkage-specific sialic acid information.					
32898301	7	15	from	RESULTS	986:992	arg1	patients					1002:1009	PDAC patients	997:1009	PDAC patients	997:1009	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	10	16	theme	PDAC	1622:1625	arg1	patients					1627:1634	PDAC patients	1622:1634	PDAC patients from healthy controls	1622:1656	CONCLUSION Serum N-glycome analysis revealed glycosylation differences that allow classification of PDAC patients from healthy controls.					
32898301	5	17	theme	N-glycan	679:686	arg1	profiles					688:695	Protein N-glycan profiles	671:695	Protein N-glycan profiles	671:695	Protein N-glycan profiles were obtained using ultrahigh resolution mass spectrometry and included linkage-specific sialic acid information.					
32898301	9	18	theme	classification	1269:1282	arg1	model					1284:1288	A classification model	1267:1288	A classification model built with three glycosylation traits	1267:1326	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	2	19	theme	Novel	229:233	arg1	tools					246:250	Novel diagnostic tools	229:250	Novel diagnostic tools	229:250	Novel diagnostic tools are needed to improve detection of PDAC and increase patient survival.					
32898301	9	20	theme	0.75	1484:1487	arg1	values					1434:1439	sensitivity and specificity values	1406:1439	sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set	1406:1519	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	9	21	theme	built	1290:1294	arg1	model					1284:1288	A classification model	1267:1288	A classification model built with three glycosylation traits	1267:1326	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	1	22	theme	aggressive	134:143	arg1	type					152:155	an aggressive cancer type	131:155	an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable	131:226	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	22	theme	aggressive	134:143	arg1	adenocarcinoma					106:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma	71:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC)	71:126	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	11	23	theme	clinical	1761:1768	arg1	study					1780:1784	this clinical glycomics study	1756:1784	this clinical glycomics study	1756:1784	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	5	24	theme	sialic	786:791	arg1	information					798:808	linkage-specific sialic acid information	769:808	linkage-specific sialic acid information	769:808	Protein N-glycan profiles were obtained using ultrahigh resolution mass spectrometry and included linkage-specific sialic acid information.					
32898301	7	25	theme	branching	1029:1037	arg1	level					1020:1024	a higher level	1011:1024	a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans	1011:1142	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	1	26	theme	loco-regional	162:174	arg1	spread					176:181	loco-regional spread	162:181	loco-regional spread	162:181	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	3	27	theme	N-glycan	362:369	arg1	profiles					371:378	serum protein N-glycan profiles	348:378	serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel	348:470	In this study we explore serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel.					
32898301	1	28	with	type	152:155	arg1	spread					176:181	loco-regional spread	162:181	loco-regional spread	162:181	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	29	theme	BACKGROUND	71:80	arg1	adenocarcinoma					106:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma	71:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC)	71:126	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	29	theme	BACKGROUND	71:80	arg1	type					152:155	an aggressive cancer type	131:155	an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable	131:226	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	29	theme	BACKGROUND	71:80	arg1	PDAC					122:125	PDAC	122:125	PDAC	122:125	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	3	30	theme	serum	348:352	arg1	profiles					371:378	serum protein N-glycan profiles	348:378	serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel	348:470	In this study we explore serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel.					
32898301	4	31	dep	PDAC	547:550	arg1	controls					561:568	cases/84 controls	552:568	86 PDAC cases/84 controls	544:568	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	7	32	theme	PDAC	997:1000	arg1	patients					1002:1009	PDAC patients	997:1009	PDAC patients	997:1009	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	1	33	theme	Pancreatic	88:97	arg1	adenocarcinoma					106:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma	71:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC)	71:126	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	33	theme	Pancreatic	88:97	arg1	type					152:155	an aggressive cancer type	131:155	an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable	131:226	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	33	theme	Pancreatic	88:97	arg1	PDAC					122:125	PDAC	122:125	PDAC	122:125	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	4	34	theme	serum	487:491	arg1	analysis					503:510	Total serum N-glycome analysis	481:510	Total serum N-glycome analysis	481:510	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	0	35	theme	Serum	0:4	arg1	analysis					16:23	Serum N-Glycome analysis	0:23	Serum N-Glycome analysis	0:23	Serum N-Glycome analysis reveals pancreatic cancer disease signatures.					
32898301	11	36	theme	N-glycan	1724:1731	arg1	traits					1698:1703	glycosylation traits	1684:1703	glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study	1684:1784	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	11	36	theme	N-glycan	1724:1731	arg1	structures					1733:1742	single N-glycan structures	1717:1742	glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study	1684:1784	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	11	36	theme	N-glycan	1724:1731	arg1	basis					1801:1805	a basis	1799:1805	a basis for further development of a blood-based diagnostic test	1799:1862	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	3	37	theme	disease	448:454	arg1	panel					466:470	a disease biomarker panel	446:470	a disease biomarker panel	446:470	In this study we explore serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel.					
32898301	0	38	theme	pancreatic	33:42	arg1	signatures					59:68	pancreatic cancer disease signatures	33:68	pancreatic cancer disease signatures	33:68	Serum N-Glycome analysis reveals pancreatic cancer disease signatures.					
32898301	9	39	theme	independent	1366:1376	arg1	validation					1378:1387	independent validation	1366:1387	independent validation (AUC 0.81)	1366:1398	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	9	39	theme	independent	1366:1376	arg1	AUC					1390:1392	AUC 0.81	1390:1397	AUC 0.81	1390:1397	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	6	40	theme	glycosylation	915:927	arg1	sialylation					973:983	sialylation	973:983	sialylation	973:983	N-glycans were relatively quantified and case-control classification performance was evaluated based on glycosylation traits such as branching, fucosylation, and sialylation.					
32898301	6	40	theme	glycosylation	915:927	arg1	fucosylation					955:966	fucosylation	955:966	fucosylation	955:966	N-glycans were relatively quantified and case-control classification performance was evaluated based on glycosylation traits such as branching, fucosylation, and sialylation.					
32898301	6	40	theme	glycosylation	915:927	arg1	traits					929:934	glycosylation traits	915:934	glycosylation traits such as branching, fucosylation, and sialylation	915:983	N-glycans were relatively quantified and case-control classification performance was evaluated based on glycosylation traits such as branching, fucosylation, and sialylation.					
32898301	6	40	theme	glycosylation	915:927	arg1	branching					944:952	branching	944:952	branching	944:952	N-glycans were relatively quantified and case-control classification performance was evaluated based on glycosylation traits such as branching, fucosylation, and sialylation.					
32898301	0	41	theme	disease	51:57	arg1	signatures					59:68	pancreatic cancer disease signatures	33:68	pancreatic cancer disease signatures	33:68	Serum N-Glycome analysis reveals pancreatic cancer disease signatures.					
32898301	10	42	theme	N-glycome	1539:1547	arg1	analysis					1549:1556	CONCLUSION Serum N-glycome analysis	1522:1556	CONCLUSION Serum N-glycome analysis	1522:1556	CONCLUSION Serum N-glycome analysis revealed glycosylation differences that allow classification of PDAC patients from healthy controls.					
32898301	8	43	theme	healthy	1249:1255	arg1	controls					1257:1264	healthy controls	1249:1264	healthy controls	1249:1264	Furthermore, the ratio of α2,6- vs α2,3-linked sialylation was higher in patients compared to healthy controls.					
32898301	10	44	theme	CONCLUSION	1522:1531	arg1	analysis					1549:1556	CONCLUSION Serum N-glycome analysis	1522:1556	CONCLUSION Serum N-glycome analysis	1522:1556	CONCLUSION Serum N-glycome analysis revealed glycosylation differences that allow classification of PDAC patients from healthy controls.					
32898301	9	45	theme	sensitivity	1406:1416	arg1	values					1434:1439	sensitivity and specificity values	1406:1439	sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set	1406:1519	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	6	46	theme	case-control	852:863	arg1	performance					880:890	case-control classification performance	852:890	case-control classification performance	852:890	N-glycans were relatively quantified and case-control classification performance was evaluated based on glycosylation traits such as branching, fucosylation, and sialylation.					
32898301	9	47	theme	specificity	1422:1432	arg1	values					1434:1439	sensitivity and specificity values	1406:1439	sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set	1406:1519	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	10	48	from	controls	1649:1656	arg1	classification					1604:1617	classification	1604:1617	classification of PDAC patients from healthy controls	1604:1656	CONCLUSION Serum N-glycome analysis revealed glycosylation differences that allow classification of PDAC patients from healthy controls.					
32898301	10	48	from	controls	1649:1656	arg1	patients					1627:1634	PDAC patients	1622:1634	PDAC patients from healthy controls	1622:1656	CONCLUSION Serum N-glycome analysis revealed glycosylation differences that allow classification of PDAC patients from healthy controls.					
32898301	2	49	theme	PDAC	287:290	arg1	detection					274:282	detection	274:282	detection of PDAC	274:290	Novel diagnostic tools are needed to improve detection of PDAC and increase patient survival.					
32898301	8	50	theme	α2,6-	1181:1185	arg1	higher					1218:1223	higher	1218:1223	higher	1218:1223	Furthermore, the ratio of α2,6- vs α2,3-linked sialylation was higher in patients compared to healthy controls.					
32898301	8	50	theme	α2,6-	1181:1185	arg1	ratio					1172:1176	the ratio	1168:1176	the ratio of α2,6- vs α2,3-linked sialylation	1168:1212	Furthermore, the ratio of α2,6- vs α2,3-linked sialylation was higher in patients compared to healthy controls.					
32898301	4	51	theme	collected	644:652	arg1	specimens					660:668	in-house collected serum specimens	635:668	in-house collected serum specimens	635:668	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	8	52	theme	α2,3-linked	1190:1200	arg1	sialylation					1202:1212	α2,3-linked sialylation	1190:1212	α2,3-linked sialylation	1190:1212	Furthermore, the ratio of α2,6- vs α2,3-linked sialylation was higher in patients compared to healthy controls.					
32898301	7	53	gly	fucosylation	1085:1096	arg1	N-glycans					1134:1142	N-glycans	1134:1142	N-glycans	1134:1142	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	10	54	theme	healthy	1641:1647	arg1	controls					1649:1656	healthy controls	1641:1656	healthy controls	1641:1656	CONCLUSION Serum N-glycome analysis revealed glycosylation differences that allow classification of PDAC patients from healthy controls.					
32898301	11	55	theme	further	1811:1817	arg1	development					1819:1829	further development	1811:1829	further development of a blood-based diagnostic test	1811:1862	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	7	56	theme	fucosylation	1085:1096	arg1	level					1020:1024	a higher level	1011:1024	a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans	1011:1142	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	1	57	theme	unresectable	215:226	arg1	tumor					198:202	the tumor	194:202	the tumor surgically unresectable	194:226	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	10	58	theme	patients	1627:1634	arg1	classification					1604:1617	classification	1604:1617	classification of PDAC patients from healthy controls	1604:1656	CONCLUSION Serum N-glycome analysis revealed glycosylation differences that allow classification of PDAC patients from healthy controls.					
32898301	11	59	theme	test	1859:1862	arg1	development					1819:1829	further development	1811:1829	further development of a blood-based diagnostic test	1811:1862	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	7	60	theme	antenna	1077:1083	arg1	fucosylation					1085:1096	(antenna)fucosylation	1076:1096	(antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 )	1076:1129	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	2	61	theme	diagnostic	235:244	arg1	tools					246:250	Novel diagnostic tools	229:250	Novel diagnostic tools	229:250	Novel diagnostic tools are needed to improve detection of PDAC and increase patient survival.					
32898301	11	62	theme	blood-based	1836:1846	arg1	test					1859:1862	a blood-based diagnostic test	1834:1862	a blood-based diagnostic test	1834:1862	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	5	63	theme	linkage-specific	769:784	arg1	information					798:808	linkage-specific sialic acid information	769:808	linkage-specific sialic acid information	769:808	Protein N-glycan profiles were obtained using ultrahigh resolution mass spectrometry and included linkage-specific sialic acid information.					
32898301	1	64	theme	cancer	145:150	arg1	type					152:155	an aggressive cancer type	131:155	an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable	131:226	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	64	theme	cancer	145:150	arg1	adenocarcinoma					106:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma	71:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC)	71:126	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	7	65	dep	RESULTS	986:992	arg1	found					1148:1152	found	1148:1152	was found	1144:1152	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	11	66	theme	glycomics	1770:1778	arg1	study					1780:1784	this clinical glycomics study	1756:1784	this clinical glycomics study	1756:1784	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	5	67	theme	acid	793:796	arg1	information					798:808	linkage-specific sialic acid information	769:808	linkage-specific sialic acid information	769:808	Protein N-glycan profiles were obtained using ultrahigh resolution mass spectrometry and included linkage-specific sialic acid information.					
32898301	9	68	theme	glycosylation	1307:1319	arg1	traits					1321:1326	three glycosylation traits	1301:1326	three glycosylation traits	1301:1326	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	9	69	theme	validation	1506:1515	arg1	set					1517:1519	the validation set	1502:1519	the validation set	1502:1519	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	9	70	used	used	1332:1335	arg2	model					1284:1288	A classification model	1267:1288	A classification model built with three glycosylation traits	1267:1326	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	5	71	theme	mass	738:741	arg1	spectrometry					743:754	ultrahigh resolution mass spectrometry	717:754	ultrahigh resolution mass spectrometry	717:754	Protein N-glycan profiles were obtained using ultrahigh resolution mass spectrometry and included linkage-specific sialic acid information.					
32898301	4	72	theme	Total	481:485	arg1	analysis					503:510	Total serum N-glycome analysis	481:510	Total serum N-glycome analysis	481:510	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	9	73	with	built	1290:1294	arg1	traits					1321:1326	three glycosylation traits	1301:1326	three glycosylation traits	1301:1326	A classification model built with three glycosylation traits was used for discovery (AUC 0.88) and independent validation (AUC 0.81), with sensitivity and specificity values of 0.85 and 0.71 for the discovery set and 0.75 and 0.72 for the validation set.					
32898301	7	74	theme	higher	1013:1018	arg1	level					1020:1024	a higher level	1011:1024	a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans	1011:1142	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	1	75	theme	&AIMS	82:86	arg1	adenocarcinoma					106:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma	71:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC)	71:126	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	75	theme	&AIMS	82:86	arg1	type					152:155	an aggressive cancer type	131:155	an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable	131:226	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	75	theme	&AIMS	82:86	arg1	PDAC					122:125	PDAC	122:125	PDAC	122:125	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	3	76	theme	protein	354:360	arg1	profiles					371:378	serum protein N-glycan profiles	348:378	serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel	348:470	In this study we explore serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel.					
32898301	4	77	theme	N-glycome	493:501	arg1	analysis					503:510	Total serum N-glycome analysis	481:510	Total serum N-glycome analysis	481:510	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	0	78	theme	N-Glycome	6:14	arg1	analysis					16:23	Serum N-Glycome analysis	0:23	Serum N-Glycome analysis	0:23	Serum N-Glycome analysis reveals pancreatic cancer disease signatures.					
32898301	7	79	dep	fucosylation	1085:1096	arg1	P-value					1109:1115	P-value	1109:1115	P-value	1109:1115	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	7	79	dep	fucosylation	1085:1096	arg1	13.27					1102:1106	13.27	1102:1106	13.27	1102:1106	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	1	80	theme	ductal	99:104	arg1	adenocarcinoma					106:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma	71:119	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC)	71:126	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	80	theme	ductal	99:104	arg1	type					152:155	an aggressive cancer type	131:155	an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable	131:226	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	1	80	theme	ductal	99:104	arg1	PDAC					122:125	PDAC	122:125	PDAC	122:125	BACKGROUND &AIMS Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer type with loco-regional spread that makes the tumor surgically unresectable.					
32898301	5	81	theme	ultrahigh	717:725	arg1	spectrometry					743:754	ultrahigh resolution mass spectrometry	717:754	ultrahigh resolution mass spectrometry	717:754	Protein N-glycan profiles were obtained using ultrahigh resolution mass spectrometry and included linkage-specific sialic acid information.					
32898301	7	82	dep	branching	1029:1037	arg1	P-value					1049:1055	P-value	1049:1055	P-value	1049:1055	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	7	82	dep	branching	1029:1037	arg1	6.19					1043:1046	6.19	1043:1046	6.19	1043:1046	RESULTS In PDAC patients a higher level of branching (OR 6.19, P-value 9.21 × 10-11 ) and (antenna)fucosylation (OR 13.27, P-value 2.31 × 10-9 ) of N-glycans was found.					
32898301	8	83	link	α2,3-linked	1190:1200	arg1	sialylation					1202:1212	α2,3-linked sialylation	1190:1212	α2,3-linked sialylation	1190:1212	Furthermore, the ratio of α2,6- vs α2,3-linked sialylation was higher in patients compared to healthy controls.					
32898301	3	84	theme	PDAC	385:388	arg1	patients					390:397	PDAC patients	385:397	PDAC patients with regard to their applicability to serve as a disease biomarker panel	385:470	In this study we explore serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel.					
32898301	11	85	theme	single	1717:1722	arg1	traits					1698:1703	glycosylation traits	1684:1703	glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study	1684:1784	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	11	85	theme	single	1717:1722	arg1	structures					1733:1742	single N-glycan structures	1717:1742	glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study	1684:1784	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	11	85	theme	single	1717:1722	arg1	basis					1801:1805	a basis	1799:1805	a basis for further development of a blood-based diagnostic test	1799:1862	It was demonstrated that glycosylation traits rather than single N-glycan structures obtained in this clinical glycomics study can serve as a basis for further development of a blood-based diagnostic test.					
32898301	0	86	theme	cancer	44:49	arg1	signatures					59:68	pancreatic cancer disease signatures	33:68	pancreatic cancer disease signatures	33:68	Serum N-Glycome analysis reveals pancreatic cancer disease signatures.					
32898301	4	87	dep	METHODS	473:479	arg1	applied					516:522	applied	516:522	was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens	512:668	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	4	88	theme	discovery	529:537	arg1	set					539:541	a discovery set	527:541	a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls)	527:627	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	4	88	theme	discovery	529:537	arg1	PDAC					547:550	86 PDAC cases/84 controls	544:568	86 PDAC cases/84 controls	544:568	METHODS Total serum N-glycome analysis was applied to a discovery set (86 PDAC cases/84 controls) followed by independent validation (26 cases/26 controls) using in-house collected serum specimens.					
32898301	3	89	theme	biomarker	456:464	arg1	panel					466:470	a disease biomarker panel	446:470	a disease biomarker panel	446:470	In this study we explore serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel.					
32898301	3	90	from	patients	390:397	arg1	profiles					371:378	serum protein N-glycan profiles	348:378	serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel	348:470	In this study we explore serum protein N-glycan profiles from PDAC patients with regard to their applicability to serve as a disease biomarker panel.					
32898301	10	91	theme	Serum	1533:1537	arg1	analysis					1549:1556	CONCLUSION Serum N-glycome analysis	1522:1556	CONCLUSION Serum N-glycome analysis	1522:1556	CONCLUSION Serum N-glycome analysis revealed glycosylation differences that allow classification of PDAC patients from healthy controls.					
33631789	6	0	theme	fucose	995:1000	arg1	content					984:990	the content	980:990	the content of fucose or sialic acid	980:1015	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	6	1	link	linked	1278:1283	arg1	acid					1292:1295	linked sialic acid	1278:1295	linked sialic acid	1278:1295	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	4	2	theme	anti-TNF-alpha	638:651	arg1	antibody					653:660	anti-TNF-alpha antibody	638:660	anti-TNF-alpha antibody	638:660	We investigated whether specific glycosylation patterns of the Fc-fragment would affect the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells.					
33631789	10	3	theme	Fuc-G0	1945:1950	arg1	antibodies					1952:1961	Fuc-G0 antibodies	1945:1961	Fuc-G0 antibodies	1945:1961	Only IL-2 and IL-17 levels were downregulated, and IL-5 production was upregulated by uptake of Fuc-G0 antibodies, as compared to control without antibodies.					
33631789	5	4	theme	specific	723:730	arg1	pattern					749:755	a specific Fc-glycosylation pattern	721:755	a specific Fc-glycosylation pattern	721:755	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	11	5	theme	specific	2023:2030	arg1	modification					2032:2043	The specific modification	2019:2043	The specific modification in the Fc-glycosylation pattern of anti-TNF-alpha Abs	2019:2097	CONCLUSIONS The specific modification in the Fc-glycosylation pattern of anti-TNF-alpha Abs does not affect their immunogenicity under the tested conditions.					
33631789	1	6	theme	bowel	296:300	arg1	disease					302:308	inflammatory bowel disease	283:308	inflammatory bowel disease	283:308	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	6	7	dep	[2	1177:1178	arg1	Fuc-G2S1/G2S2					1182:1194	Fuc-G2S1/G2S2	1182:1194	[2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid	1177:1240	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	11	8	theme	Fc-glycosylation	2052:2067	arg1	pattern					2069:2075	the Fc-glycosylation pattern	2048:2075	the Fc-glycosylation pattern of anti-TNF-alpha Abs	2048:2097	CONCLUSIONS The specific modification in the Fc-glycosylation pattern of anti-TNF-alpha Abs does not affect their immunogenicity under the tested conditions.					
33631789	1	9	theme	necrosis	214:221	arg1	factor-alpha					223:234	tumor necrosis factor-alpha	208:234	tumor necrosis factor-alpha [TNF-alpha]	208:246	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	11	10	theme	anti-TNF-alpha	2080:2093	arg1	Abs					2095:2097	anti-TNF-alpha Abs	2080:2097	anti-TNF-alpha Abs	2080:2097	CONCLUSIONS The specific modification in the Fc-glycosylation pattern of anti-TNF-alpha Abs does not affect their immunogenicity under the tested conditions.					
33631789	6	11	theme	[2	1177:1178	arg1	acid					1171:1174	sialic acid	1164:1174	sialic acid	1164:1174	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	7	12	gly	sialylation	1374:1384	arg1	anti-TNF-Abs					1389:1400	anti-TNF-Abs [Fuc-G0	1389:1408	anti-TNF-Abs [Fuc-G0	1389:1408	RESULTS Our data demonstrated that neither fucosylation nor sialylation of anti-TNF-Abs [Fuc-G0, FucG2S1/G2S2, G2S1/G2S2] influence their uptake by mo-DCs.					
33631789	9	13	theme	cytokines	1687:1695	arg1	levels					1677:1682	the levels	1673:1682	the levels of cytokines	1673:1695	Next, we evaluated the levels of cytokines in the supernatant of mo-DCs stimulated with lipopolysaccharide in the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies.					
33631789	5	14	theme	dendritic	796:804	arg1	cells					806:810	monocyte-derived dendritic cells	779:810	monocyte-derived dendritic cells [mo-DCs	779:818	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	6	15	theme	linked	1278:1283	arg1	acid					1292:1295	linked sialic acid	1278:1295	linked sialic acid	1278:1295	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	6	16	link	linked	1223:1228	arg1	acid					1237:1240	linked sialic acid	1223:1240	linked sialic acid	1223:1240	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	5	17	theme	pattern	749:755	arg1	effect					711:716	The effect	707:716	The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs]	707:819	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	11	18	dep	CONCLUSIONS	2007:2017	arg1	affect					2108:2113	affect	2108:2113	does not affect their immunogenicity under the tested conditions	2099:2162	CONCLUSIONS The specific modification in the Fc-glycosylation pattern of anti-TNF-alpha Abs does not affect their immunogenicity under the tested conditions.					
33631789	6	19	theme	sialic	1005:1010	arg1	acid					1012:1015	sialic acid	1005:1015	sialic acid	1005:1015	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	6	20	theme	linked	1223:1228	arg1	acid					1237:1240	linked sialic acid	1223:1240	linked sialic acid	1223:1240	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	5	21	theme	antibody	760:767	arg1	uptake					769:774	antibody uptake	760:774	antibody uptake by monocyte-derived dendritic cells [mo-DCs	760:818	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	0	22	theme	Factor	68:73	arg1	Alpha					75:79	anti-Tumour Necrosis Factor Alpha	47:79	anti-Tumour Necrosis Factor Alpha Antibodies	47:90	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	3	23	theme	antibodies	432:441	arg1	glycosylation					415:427	glycosylation	415:427	glycosylation of antibodies	415:441	On the other hand, glycosylation of antibodies might influence not only their immunogenicity but also their structure and function.					
33631789	1	24	theme	BACKGROUND	167:176	arg1	Antibodies					187:196	BACKGROUND AND AIMS Antibodies	167:196	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha]	167:246	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	1	24	theme	BACKGROUND	167:176	arg1	mainstay					254:261	a mainstay	252:261	a mainstay in the treatment of inflammatory bowel disease	252:308	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	12	25	theme	immunological	2294:2306	arg1	profile					2308:2314	the immunological profile	2290:2314	the immunological profile of T- and B-cells	2290:2332	As this study was limited to mo-DCs, further investigation is required to clarify whether Ab uptake into mo-DCs might change the immunological profile of T- and B-cells, in order to ultimately reduce the formation of anti-drug antibodies and to improve the patient care.					
33631789	8	26	from	lipopolysaccharide	1604:1621	arg1	presence					1630:1637	the presence	1626:1637	the presence of antibodies	1626:1651	Additionally, none of the differentially glycosylated antibodies altered CD80, CD86, CD273, CD274 levels on mo-DCs stimulated in with lipopolysaccharide in the presence of antibodies.					
33631789	0	27	theme	Antibodies	81:90	arg1	Fc-Fragment					32:42	Fc-Fragment	32:42	Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies	32:90	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	1	28	theme	AIMS	182:185	arg1	Antibodies					187:196	BACKGROUND AND AIMS Antibodies	167:196	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha]	167:246	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	1	28	theme	AIMS	182:185	arg1	mainstay					254:261	a mainstay	252:261	a mainstay in the treatment of inflammatory bowel disease	252:308	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	12	29	theme	Ab	2255:2256	arg1	uptake					2258:2263	Ab uptake	2255:2263	Ab uptake into mo-DCs	2255:2275	As this study was limited to mo-DCs, further investigation is required to clarify whether Ab uptake into mo-DCs might change the immunological profile of T- and B-cells, in order to ultimately reduce the formation of anti-drug antibodies and to improve the patient care.					
33631789	9	30	gly	G2S1/G2S2-glycosylated	1805:1826	arg1	antibodies					1837:1846	G2S1/G2S2-glycosylated anti-TNF antibodies	1805:1846	G2S1/G2S2-glycosylated anti-TNF antibodies	1805:1846	Next, we evaluated the levels of cytokines in the supernatant of mo-DCs stimulated with lipopolysaccharide in the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies.					
33631789	8	31	theme	glycosylated	1511:1522	arg1	antibodies					1524:1533	the differentially glycosylated antibodies	1492:1533	the differentially glycosylated antibodies	1492:1533	Additionally, none of the differentially glycosylated antibodies altered CD80, CD86, CD273, CD274 levels on mo-DCs stimulated in with lipopolysaccharide in the presence of antibodies.					
33631789	9	32	theme	anti-TNF	1828:1835	arg1	antibodies					1837:1846	G2S1/G2S2-glycosylated anti-TNF antibodies	1805:1846	G2S1/G2S2-glycosylated anti-TNF antibodies	1805:1846	Next, we evaluated the levels of cytokines in the supernatant of mo-DCs stimulated with lipopolysaccharide in the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies.					
33631789	3	33	theme	other	403:407	arg1	hand					409:412	the other hand	399:412	the other hand	399:412	On the other hand, glycosylation of antibodies might influence not only their immunogenicity but also their structure and function.					
33631789	6	34	gly	N-glycoforms	907:918	arg1	adalimumab					951:960	the anti-TNF-alpha antibody adalimumab	923:960	the anti-TNF-alpha antibody adalimumab	923:960	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	1	35	theme	disease	302:308	arg1	treatment					270:278	the treatment	266:278	the treatment of inflammatory bowel disease	266:308	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	4	36	link	monocyte-derived	665:680	arg1	cells					692:696	monocyte-derived dendritic cells	665:696	monocyte-derived dendritic cells	665:696	We investigated whether specific glycosylation patterns of the Fc-fragment would affect the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells.					
33631789	12	37	theme	patient	2422:2428	arg1	care					2430:2433	the patient care	2418:2433	the patient care	2418:2433	As this study was limited to mo-DCs, further investigation is required to clarify whether Ab uptake into mo-DCs might change the immunological profile of T- and B-cells, in order to ultimately reduce the formation of anti-drug antibodies and to improve the patient care.					
33631789	6	38	dep	fucose	1115:1120	arg1	[GlcNAc					1146:1152	[GlcNAc	1146:1152	[GlcNAc	1146:1152	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	12	39	theme	anti-drug	2382:2390	arg1	antibodies					2392:2401	anti-drug antibodies	2382:2401	anti-drug antibodies	2382:2401	As this study was limited to mo-DCs, further investigation is required to clarify whether Ab uptake into mo-DCs might change the immunological profile of T- and B-cells, in order to ultimately reduce the formation of anti-drug antibodies and to improve the patient care.					
33631789	5	40	dep	METHODS	699:705	arg1	effect					711:716	The effect	707:716	The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs]	707:819	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	5	40	dep	METHODS	699:705	arg1	shapes					842:847	shapes	842:847	shapes the immunologic profile of mo-DCs	842:881	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	10	41	theme	IL-17	1863:1867	arg1	levels					1869:1874	Only IL-2 and IL-17 levels	1849:1874	Only IL-2 and IL-17 levels	1849:1874	Only IL-2 and IL-17 levels were downregulated, and IL-5 production was upregulated by uptake of Fuc-G0 antibodies, as compared to control without antibodies.					
33631789	5	42	theme	immunologic	853:863	arg1	profile					865:871	the immunologic profile	849:871	the immunologic profile of mo-DCs	849:881	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	0	43	theme	Fc-Fragment	32:42	arg1	Sialylation					17:27	Sialylation	17:27	Sialylation	17:27	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	0	43	theme	Fc-Fragment	32:42	arg1	Fucosylation					0:11	Fucosylation	0:11	Fucosylation	0:11	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	6	44	theme	antibody	942:949	arg1	adalimumab					951:960	the anti-TNF-alpha antibody adalimumab	923:960	the anti-TNF-alpha antibody adalimumab	923:960	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	4	45	theme	specific	552:559	arg1	patterns					575:582	specific glycosylation patterns	552:582	specific glycosylation patterns of the Fc-fragment	552:601	We investigated whether specific glycosylation patterns of the Fc-fragment would affect the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells.					
33631789	10	46	theme	IL-2	1854:1857	arg1	levels					1869:1874	Only IL-2 and IL-17 levels	1849:1874	Only IL-2 and IL-17 levels	1849:1874	Only IL-2 and IL-17 levels were downregulated, and IL-5 production was upregulated by uptake of Fuc-G0 antibodies, as compared to control without antibodies.					
33631789	2	47	theme	considerable	359:370	arg1	proportion					372:381	a considerable proportion	357:381	a considerable proportion of patients	357:393	However, they fail to demonstrate efficacy in a considerable proportion of patients.					
33631789	0	48	theme	anti-Tumour	47:57	arg1	Alpha					75:79	anti-Tumour Necrosis Factor Alpha	47:79	anti-Tumour Necrosis Factor Alpha Antibodies	47:90	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	5	49	from	effect	711:716	arg1	uptake					769:774	antibody uptake	760:774	antibody uptake by monocyte-derived dendritic cells [mo-DCs	760:818	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	7	50	dep	RESULTS	1314:1320	arg1	demonstrated					1331:1342	demonstrated	1331:1342	demonstrated that neither fucosylation nor sialylation of anti-TNF-Abs [Fuc-G0, FucG2S1/G2S2, G2S1/G2S2] influence their uptake by mo-DCs	1331:1467	RESULTS Our data demonstrated that neither fucosylation nor sialylation of anti-TNF-Abs [Fuc-G0, FucG2S1/G2S2, G2S1/G2S2] influence their uptake by mo-DCs.					
33631789	6	51	theme	sialic	1164:1169	arg1	acid					1171:1174	sialic acid	1164:1174	sialic acid	1164:1174	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	11	52	from	modification	2032:2043	arg1	pattern					2069:2075	the Fc-glycosylation pattern	2048:2075	the Fc-glycosylation pattern of anti-TNF-alpha Abs	2048:2097	CONCLUSIONS The specific modification in the Fc-glycosylation pattern of anti-TNF-alpha Abs does not affect their immunogenicity under the tested conditions.					
33631789	4	53	theme	antibody	653:660	arg1	immunogenicity					620:633	the immunogenicity	616:633	the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells	616:696	We investigated whether specific glycosylation patterns of the Fc-fragment would affect the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells.					
33631789	1	54	dep	factor-alpha	223:234	arg1	[TNF-alpha					236:245	[TNF-alpha	236:245	[TNF-alpha	236:245	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	5	55	link	monocyte-derived	779:794	arg1	cells					806:810	monocyte-derived dendritic cells	779:810	monocyte-derived dendritic cells [mo-DCs	779:818	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	4	56	theme	monocyte-derived	665:680	arg1	cells					692:696	monocyte-derived dendritic cells	665:696	monocyte-derived dendritic cells	665:696	We investigated whether specific glycosylation patterns of the Fc-fragment would affect the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells.					
33631789	11	57	theme	tested	2146:2151	arg1	conditions					2153:2162	the tested conditions	2142:2162	the tested conditions	2142:2162	CONCLUSIONS The specific modification in the Fc-glycosylation pattern of anti-TNF-alpha Abs does not affect their immunogenicity under the tested conditions.					
33631789	10	58	theme	IL-5	1900:1903	arg1	production					1905:1914	IL-5 production	1900:1914	IL-5 production	1900:1914	Only IL-2 and IL-17 levels were downregulated, and IL-5 production was upregulated by uptake of Fuc-G0 antibodies, as compared to control without antibodies.					
33631789	1	59	theme	inflammatory	283:294	arg1	disease					302:308	inflammatory bowel disease	283:308	inflammatory bowel disease	283:308	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	1	60	theme	tumor	208:212	arg1	factor-alpha					223:234	tumor necrosis factor-alpha	208:234	tumor necrosis factor-alpha [TNF-alpha]	208:246	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	0	61	theme	Monocyte-Derived	133:148	arg1	Cells					160:164	Monocyte-Derived Dendritic Cells	133:164	Monocyte-Derived Dendritic Cells	133:164	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	11	62	theme	Abs	2095:2097	arg1	pattern					2069:2075	the Fc-glycosylation pattern	2048:2075	the Fc-glycosylation pattern of anti-TNF-alpha Abs	2048:2097	CONCLUSIONS The specific modification in the Fc-glycosylation pattern of anti-TNF-alpha Abs does not affect their immunogenicity under the tested conditions.					
33631789	7	63	theme	anti-TNF-Abs	1389:1400	arg1	G2S1/G2S2					1425:1433	G2S1/G2S2	1425:1433	G2S1/G2S2	1425:1433	RESULTS Our data demonstrated that neither fucosylation nor sialylation of anti-TNF-Abs [Fuc-G0, FucG2S1/G2S2, G2S1/G2S2] influence their uptake by mo-DCs.					
33631789	7	63	theme	anti-TNF-Abs	1389:1400	arg1	FucG2S1/G2S2					1411:1422	FucG2S1/G2S2	1411:1422	FucG2S1/G2S2	1411:1422	RESULTS Our data demonstrated that neither fucosylation nor sialylation of anti-TNF-Abs [Fuc-G0, FucG2S1/G2S2, G2S1/G2S2] influence their uptake by mo-DCs.					
33631789	7	63	theme	anti-TNF-Abs	1389:1400	arg1	sialylation					1374:1384	sialylation	1374:1384	sialylation of anti-TNF-Abs [Fuc-G0	1374:1408	RESULTS Our data demonstrated that neither fucosylation nor sialylation of anti-TNF-Abs [Fuc-G0, FucG2S1/G2S2, G2S1/G2S2] influence their uptake by mo-DCs.					
33631789	10	64	theme	antibodies	1952:1961	arg1	uptake					1935:1940	uptake	1935:1940	uptake of Fuc-G0 antibodies	1935:1961	Only IL-2 and IL-17 levels were downregulated, and IL-5 production was upregulated by uptake of Fuc-G0 antibodies, as compared to control without antibodies.					
33631789	5	65	theme	monocyte-derived	779:794	arg1	cells					806:810	monocyte-derived dendritic cells	779:810	monocyte-derived dendritic cells [mo-DCs	779:818	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	6	66	with	[2	1177:1178	arg1	alpha					1213:1217	alpha	1213:1217	alpha	1213:1217	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	6	66	with	[2	1177:1178	arg1	fucose					1202:1207	fucose	1202:1207	fucose	1202:1207	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	5	67	theme	Fc-glycosylation	732:747	arg1	pattern					749:755	a specific Fc-glycosylation pattern	721:755	a specific Fc-glycosylation pattern	721:755	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	6	68	theme	acid	1012:1015	arg1	content					984:990	the content	980:990	the content of fucose or sialic acid	980:1015	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	0	69	from	Immunogenicity	115:128	arg1	Cells					160:164	Monocyte-Derived Dendritic Cells	133:164	Monocyte-Derived Dendritic Cells	133:164	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	6	70	theme	sialic	1230:1235	arg1	acid					1237:1240	linked sialic acid	1223:1240	linked sialic acid	1223:1240	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	6	71	theme	sialic	1285:1290	arg1	acid					1292:1295	linked sialic acid	1278:1295	linked sialic acid	1278:1295	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	9	72	theme	mo-DCs	1719:1724	arg1	supernatant					1704:1714	the supernatant	1700:1714	the supernatant of mo-DCs stimulated with lipopolysaccharide in the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies	1700:1846	Next, we evaluated the levels of cytokines in the supernatant of mo-DCs stimulated with lipopolysaccharide in the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies.					
33631789	0	73	theme	Alpha	75:79	arg1	Antibodies					81:90	anti-Tumour Necrosis Factor Alpha Antibodies	47:90	anti-Tumour Necrosis Factor Alpha Antibodies	47:90	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	4	74	gly	glycosylation	561:573	arg1	Fc-fragment					591:601	the Fc-fragment	587:601	the Fc-fragment	587:601	We investigated whether specific glycosylation patterns of the Fc-fragment would affect the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells.					
33631789	8	75	gly	glycosylated	1511:1522	arg1	antibodies					1524:1533	the differentially glycosylated antibodies	1492:1533	the differentially glycosylated antibodies	1492:1533	Additionally, none of the differentially glycosylated antibodies altered CD80, CD86, CD273, CD274 levels on mo-DCs stimulated in with lipopolysaccharide in the presence of antibodies.					
33631789	6	76	dep	treated	1040:1046	arg1	[1					1031:1032	[1	1031:1032	[1	1031:1032	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	12	77	theme	T-	2319:2320	arg1	profile					2308:2314	the immunological profile	2290:2314	the immunological profile of T- and B-cells	2290:2332	As this study was limited to mo-DCs, further investigation is required to clarify whether Ab uptake into mo-DCs might change the immunological profile of T- and B-cells, in order to ultimately reduce the formation of anti-drug antibodies and to improve the patient care.					
33631789	5	78	theme	cells	806:810	arg1	[mo-DCs					812:818	monocyte-derived dendritic cells [mo-DCs	779:818	monocyte-derived dendritic cells [mo-DCs	779:818	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	3	79	gly	glycosylation	415:427	arg1	antibodies					432:441	antibodies	432:441	antibodies	432:441	On the other hand, glycosylation of antibodies might influence not only their immunogenicity but also their structure and function.					
33631789	1	80	from	mainstay	254:261	arg1	treatment					270:278	the treatment	266:278	the treatment of inflammatory bowel disease	266:308	BACKGROUND AND AIMS Antibodies targeting tumor necrosis factor-alpha [TNF-alpha] are a mainstay in the treatment of inflammatory bowel disease.					
33631789	8	81	theme	antibodies	1524:1533	arg1	none					1484:1487	none	1484:1487	none of the differentially glycosylated antibodies	1484:1533	Additionally, none of the differentially glycosylated antibodies altered CD80, CD86, CD273, CD274 levels on mo-DCs stimulated in with lipopolysaccharide in the presence of antibodies.					
33631789	4	82	from	immunogenicity	620:633	arg1	cells					692:696	monocyte-derived dendritic cells	665:696	monocyte-derived dendritic cells	665:696	We investigated whether specific glycosylation patterns of the Fc-fragment would affect the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells.					
33631789	9	83	theme	Fuc-G0	1780:1785	arg1	presence					1768:1775	the presence	1764:1775	the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies	1764:1846	Next, we evaluated the levels of cytokines in the supernatant of mo-DCs stimulated with lipopolysaccharide in the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies.					
33631789	8	84	theme	CD80	1543:1546	arg1	levels					1568:1573	CD80, CD86, CD273, CD274 levels	1543:1573	CD80, CD86, CD273, CD274 levels	1543:1573	Additionally, none of the differentially glycosylated antibodies altered CD80, CD86, CD273, CD274 levels on mo-DCs stimulated in with lipopolysaccharide in the presence of antibodies.					
33631789	12	85	theme	antibodies	2392:2401	arg1	formation					2369:2377	the formation	2365:2377	the formation of anti-drug antibodies	2365:2401	As this study was limited to mo-DCs, further investigation is required to clarify whether Ab uptake into mo-DCs might change the immunological profile of T- and B-cells, in order to ultimately reduce the formation of anti-drug antibodies and to improve the patient care.					
33631789	5	86	theme	mo-DCs	876:881	arg1	profile					865:871	the immunologic profile	849:871	the immunologic profile of mo-DCs	849:881	METHODS The effect of a specific Fc-glycosylation pattern on antibody uptake by monocyte-derived dendritic cells [mo-DCs] and how this process shapes the immunologic profile of mo-DCs was investigated.					
33631789	9	87	theme	Fuc-G2S1/G2S2	1788:1800	arg1	presence					1768:1775	the presence	1764:1775	the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies	1764:1846	Next, we evaluated the levels of cytokines in the supernatant of mo-DCs stimulated with lipopolysaccharide in the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies.					
33631789	4	88	theme	Fc-fragment	591:601	arg1	patterns					575:582	specific glycosylation patterns	552:582	specific glycosylation patterns of the Fc-fragment	552:601	We investigated whether specific glycosylation patterns of the Fc-fragment would affect the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells.					
33631789	9	89	theme	G2S1/G2S2-glycosylated	1805:1826	arg1	antibodies					1837:1846	G2S1/G2S2-glycosylated anti-TNF antibodies	1805:1846	G2S1/G2S2-glycosylated anti-TNF antibodies	1805:1846	Next, we evaluated the levels of cytokines in the supernatant of mo-DCs stimulated with lipopolysaccharide in the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies.					
33631789	6	90	theme	adalimumab	951:960	arg1	N-glycoforms					907:918	Three N-glycoforms	901:918	Three N-glycoforms	901:918	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	0	91	gly	Fucosylation	0:11	arg1	Fc-Fragment					32:42	Fc-Fragment	32:42	Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies	32:90	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	9	92	theme	antibodies	1837:1846	arg1	presence					1768:1775	the presence	1764:1775	the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies	1764:1846	Next, we evaluated the levels of cytokines in the supernatant of mo-DCs stimulated with lipopolysaccharide in the presence of Fuc-G0, Fuc-G2S1/G2S2 or G2S1/G2S2-glycosylated anti-TNF antibodies.					
33631789	4	93	theme	glycosylation	561:573	arg1	patterns					575:582	specific glycosylation patterns	552:582	specific glycosylation patterns of the Fc-fragment	552:601	We investigated whether specific glycosylation patterns of the Fc-fragment would affect the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells.					
33631789	0	94	theme	Necrosis	59:66	arg1	Alpha					75:79	anti-Tumour Necrosis Factor Alpha	47:79	anti-Tumour Necrosis Factor Alpha Antibodies	47:90	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	6	95	theme	anti-TNF-alpha	927:940	arg1	adalimumab					951:960	the anti-TNF-alpha antibody adalimumab	923:960	the anti-TNF-alpha antibody adalimumab	923:960	Three N-glycoforms of the anti-TNF-alpha antibody adalimumab, that differed in the content of fucose or sialic acid, were tested: [1] mock treated Humira, abbreviated 'Fuc-G0', where the N-glycan mainly consist of fucose and N-acetylglucosamine [GlcNAc], without sialic acid; [2] 'Fuc-G2S1/G2S2' with fucose and alpha 2,6 linked sialic acid; and [3] 'G2S1/G2S2' with alpha 2,6 linked sialic acid, without fucose.					
33631789	0	96	theme	Dendritic	150:158	arg1	Cells					160:164	Monocyte-Derived Dendritic Cells	133:164	Monocyte-Derived Dendritic Cells	133:164	Fucosylation and Sialylation of Fc-Fragment of anti-Tumour Necrosis Factor Alpha Antibodies do not Influence Their Immunogenicity in Monocyte-Derived Dendritic Cells.					
33631789	12	97	theme	further	2202:2208	arg1	investigation					2210:2222	further investigation	2202:2222	further investigation	2202:2222	As this study was limited to mo-DCs, further investigation is required to clarify whether Ab uptake into mo-DCs might change the immunological profile of T- and B-cells, in order to ultimately reduce the formation of anti-drug antibodies and to improve the patient care.					
33631789	8	98	theme	antibodies	1642:1651	arg1	presence					1630:1637	the presence	1626:1637	the presence of antibodies	1626:1651	Additionally, none of the differentially glycosylated antibodies altered CD80, CD86, CD273, CD274 levels on mo-DCs stimulated in with lipopolysaccharide in the presence of antibodies.					
33631789	11	99	gly	Fc-glycosylation	2052:2067	arg1	Abs					2095:2097	anti-TNF-alpha Abs	2080:2097	anti-TNF-alpha Abs	2080:2097	CONCLUSIONS The specific modification in the Fc-glycosylation pattern of anti-TNF-alpha Abs does not affect their immunogenicity under the tested conditions.					
33631789	12	100	theme	B-cells	2326:2332	arg1	profile					2308:2314	the immunological profile	2290:2314	the immunological profile of T- and B-cells	2290:2332	As this study was limited to mo-DCs, further investigation is required to clarify whether Ab uptake into mo-DCs might change the immunological profile of T- and B-cells, in order to ultimately reduce the formation of anti-drug antibodies and to improve the patient care.					
33631789	4	101	theme	dendritic	682:690	arg1	cells					692:696	monocyte-derived dendritic cells	665:696	monocyte-derived dendritic cells	665:696	We investigated whether specific glycosylation patterns of the Fc-fragment would affect the immunogenicity of anti-TNF-alpha antibody in monocyte-derived dendritic cells.					
33631789	2	102	theme	patients	386:393	arg1	proportion					372:381	a considerable proportion	357:381	a considerable proportion of patients	357:393	However, they fail to demonstrate efficacy in a considerable proportion of patients.					
32046654	8	0	theme	putative	1164:1171	arg1	isolate					1203:1209	the putative periodontal health-associated isolate	1160:1209	the putative periodontal health-associated isolate Tannerella sp	1160:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	12	1	theme	O-glycosylation	1509:1523	arg1	cluster					1530:1536	the general protein O-glycosylation gene cluster	1489:1536	the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037	1489:1563	While the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037 has been described previously, genes required for the initiation of glycan synthesis are yet to be discovered.					
32046654	8	2	theme	health-associated	1185:1201	arg1	isolate					1203:1209	the putative periodontal health-associated isolate	1160:1209	the putative periodontal health-associated isolate Tannerella sp	1160:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	5	3	dep	T.	607:608	arg1	forsythia					610:618	forsythia	610:618	forsythia	610:618	In the past, strain confusion involving the T. forsythia reference type strain ATCC 43037 led to discrepancies between results obtained from in silico analyses and wet-lab experimentation.					
32046654	15	4	theme	biased	1899:1904	arg1	genes					1906:1910	highly biased genes	1892:1910	highly biased genes	1892:1910	BU063 and detected highly biased genes.					
32046654	2	5	theme	virulence-associated	186:205	arg1	genes					220:224	Numerous virulence-associated T. forsythia genes	177:224	Numerous virulence-associated T. forsythia genes	177:224	Numerous virulence-associated T. forsythia genes have been described, however, it is necessary to expand the knowledge on T. forsythia's genome structure and genetic repertoire to further elucidate its role within pathogenesis.					
32046654	5	6	theme	in	704:705	arg1	analyses					714:721	in silico analyses	704:721	in silico analyses	704:721	In the past, strain confusion involving the T. forsythia reference type strain ATCC 43037 led to discrepancies between results obtained from in silico analyses and wet-lab experimentation.					
32046654	7	7	theme	annotated	894:902	arg1	genomes					904:910	annotated genomes	894:910	annotated genomes of ten Tannerella strains	894:936	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	8	8	theme	Tannerella	1211:1220	arg1	sp					1222:1223	Tannerella sp	1211:1223	the putative periodontal health-associated isolate Tannerella sp	1160:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	19	9	from	virulence	2416:2424	arg1	particular					2445:2454	particular	2445:2454	particular	2445:2454	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	19	9	from	virulence	2416:2424	arg1	general					2404:2410	general	2404:2410	general	2404:2410	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	17	10	theme	putative	2146:2153	arg1	factors					2165:2171	novel putative virulence factors	2140:2171	novel putative virulence factors	2140:2171	Comparative analyses enabled us to assess the suitability of T. forsythia virulence factors as therapeutic targets and to suggest novel putative virulence factors.					
32046654	11	11	theme	pathogenicity	1460:1472	arg1	islands					1474:1480	previously described pathogenicity islands	1439:1480	previously described pathogenicity islands	1439:1480	Only four of these regions corresponded to previously described pathogenicity islands.					
32046654	18	12	dep	elucidating	2249:2259	arg1	the					2234:2236	the	2234:2236	the	2234:2236	Further, we report on gene loci that should be addressed in the context of elucidating T. forsythia's protein O-glycosylation pathway.					
32046654	18	12	dep	elucidating	2249:2259	arg1	context					2238:2244	context	2238:2244	context	2238:2244	Further, we report on gene loci that should be addressed in the context of elucidating T. forsythia's protein O-glycosylation pathway.					
32046654	12	13	theme	general	1493:1499	arg1	cluster					1530:1536	the general protein O-glycosylation gene cluster	1489:1536	the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037	1489:1563	While the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037 has been described previously, genes required for the initiation of glycan synthesis are yet to be discovered.					
32046654	5	14	theme	strain	576:581	arg1	confusion					583:591	strain confusion	576:591	strain confusion involving the T. forsythia reference type strain ATCC 43037	576:651	In the past, strain confusion involving the T. forsythia reference type strain ATCC 43037 led to discrepancies between results obtained from in silico analyses and wet-lab experimentation.					
32046654	13	15	theme	other	1756:1760	arg1	bacteria					1762:1769	other bacteria	1756:1769	other bacteria	1756:1769	We found six putative glycosylation loci which were only partially conserved in other bacteria.					
32046654	6	16	theme	ATCC	830:833	arg1	assembly					805:812	a substantially improved genome assembly	773:812	a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences	773:885	RESULTS We generated a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences.					
32046654	19	17	theme	further	2348:2354	arg1	dissection					2366:2375	further molecular dissection	2348:2375	further molecular dissection of T. forsythia biology in general	2348:2410	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	7	18	from	%	1033:1033	arg1	present					1016:1022	present	1016:1022	present	1016:1022	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	9	19	theme	T.	1270:1271	arg1	pathogenesis					1285:1296	T. forsythia's pathogenesis	1270:1296	T. forsythia's pathogenesis	1270:1296	BU063 to identify candidate genes promoting T. forsythia's pathogenesis.					
32046654	2	20	theme	genome	314:319	arg1	structure					321:329	T. forsythia's genome structure	299:329	T. forsythia's genome structure	299:329	Numerous virulence-associated T. forsythia genes have been described, however, it is necessary to expand the knowledge on T. forsythia's genome structure and genetic repertoire to further elucidate its role within pathogenesis.					
32046654	17	21	theme	Comparative	2010:2020	arg1	analyses					2022:2029	Comparative analyses	2010:2029	Comparative analyses	2010:2029	Comparative analyses enabled us to assess the suitability of T. forsythia virulence factors as therapeutic targets and to suggest novel putative virulence factors.					
32046654	12	22	theme	synthesis	1640:1648	arg1	initiation					1619:1628	the initiation	1615:1628	the initiation of glycan synthesis	1615:1648	While the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037 has been described previously, genes required for the initiation of glycan synthesis are yet to be discovered.					
32046654	4	23	theme	known	493:497	arg1	relative					499:506	closest known relative	485:506	closest known relative	485:506	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	17	24	theme	factors	2094:2100	arg1	suitability					2056:2066	the suitability	2052:2066	the suitability of T. forsythia virulence factors as therapeutic targets	2052:2123	Comparative analyses enabled us to assess the suitability of T. forsythia virulence factors as therapeutic targets and to suggest novel putative virulence factors.					
32046654	18	25	theme	T.	2261:2262	arg1	pathway					2300:2306	T. forsythia's protein O-glycosylation pathway	2261:2306	T. forsythia's protein O-glycosylation pathway	2261:2306	Further, we report on gene loci that should be addressed in the context of elucidating T. forsythia's protein O-glycosylation pathway.					
32046654	4	26	theme	periodontal	438:448	arg1	taxon					475:479	a putative periodontal health-associated sister taxon	427:479	a putative periodontal health-associated sister taxon	427:479	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	4	26	theme	periodontal	438:448	arg1	available					527:535	available	527:535	available	527:535	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	17	27	theme	therapeutic	2105:2115	arg1	targets					2117:2123	therapeutic targets	2105:2123	therapeutic targets	2105:2123	Comparative analyses enabled us to assess the suitability of T. forsythia virulence factors as therapeutic targets and to suggest novel putative virulence factors.					
32046654	5	28	theme	wet-lab	727:733	arg1	experimentation					735:749	wet-lab experimentation	727:749	wet-lab experimentation	727:749	In the past, strain confusion involving the T. forsythia reference type strain ATCC 43037 led to discrepancies between results obtained from in silico analyses and wet-lab experimentation.					
32046654	1	29	theme	periodontal	156:166	arg1	disease					168:174	periodontal disease	156:174	periodontal disease	156:174	BACKGROUND Tannerella forsythia is a bacterial pathogen implicated in periodontal disease.					
32046654	4	30	theme	sister	468:473	arg1	taxon					475:479	a putative periodontal health-associated sister taxon	427:479	a putative periodontal health-associated sister taxon	427:479	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	4	30	theme	sister	468:473	arg1	available					527:535	available	527:535	available	527:535	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	0	31	theme	forsythia	75:83	arg1	characterization					19:34	Comparative genome characterization	0:34	Comparative genome characterization of the periodontal pathogen Tannerella forsythia.	0:84	Comparative genome characterization of the periodontal pathogen Tannerella forsythia.					
32046654	17	32	theme	T.	2071:2072	arg1	factors					2094:2100	T. forsythia virulence factors	2071:2100	T. forsythia virulence factors	2071:2100	Comparative analyses enabled us to assess the suitability of T. forsythia virulence factors as therapeutic targets and to suggest novel putative virulence factors.					
32046654	7	33	attach	present	1016:1022	arg2	orthologs					1006:1014	orthologs	1006:1014	orthologs present in > = 80% of the strains analysed	1006:1057	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	7	33	attach	present	1016:1022	arg1	%					1033:1033	> = 80%	1027:1033	> = 80% of the strains analysed	1027:1057	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	7	33	attach	present	1016:1022	arg1	strains					1042:1048	the strains	1038:1048	the strains analysed	1038:1057	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	5	34	theme	type	630:633	arg1	strain					635:640	the T. forsythia reference type strain	603:640	the T. forsythia reference type strain ATCC 43037	603:651	In the past, strain confusion involving the T. forsythia reference type strain ATCC 43037 led to discrepancies between results obtained from in silico analyses and wet-lab experimentation.					
32046654	4	35	theme	comparative	541:551	arg1	analyses					553:560	comparative analyses	541:560	comparative analyses	541:560	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	1	36	theme	Tannerella	97:106	arg1	pathogen					133:140	a bacterial pathogen	121:140	a bacterial pathogen implicated in periodontal disease	121:174	BACKGROUND Tannerella forsythia is a bacterial pathogen implicated in periodontal disease.					
32046654	1	36	theme	Tannerella	97:106	arg1	forsythia					108:116	BACKGROUND Tannerella forsythia	86:116	BACKGROUND Tannerella forsythia	86:116	BACKGROUND Tannerella forsythia is a bacterial pathogen implicated in periodontal disease.					
32046654	17	37	theme	forsythia	2074:2082	arg1	factors					2094:2100	T. forsythia virulence factors	2071:2100	T. forsythia virulence factors	2071:2100	Comparative analyses enabled us to assess the suitability of T. forsythia virulence factors as therapeutic targets and to suggest novel putative virulence factors.					
32046654	0	38	theme	Comparative	0:10	arg1	characterization					19:34	Comparative genome characterization	0:34	Comparative genome characterization of the periodontal pathogen Tannerella forsythia.	0:84	Comparative genome characterization of the periodontal pathogen Tannerella forsythia.					
32046654	7	39	theme	core	960:963	arg1	genome					965:970	a soft core genome	953:970	a soft core genome encompassing 2108 genes	953:994	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	6	40	dep	RESULTS	752:758	arg1	generated					763:771	generated	763:771	generated a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences	763:885	RESULTS We generated a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences.					
32046654	1	41	theme	bacterial	123:131	arg1	pathogen					133:140	a bacterial pathogen	121:140	a bacterial pathogen implicated in periodontal disease	121:174	BACKGROUND Tannerella forsythia is a bacterial pathogen implicated in periodontal disease.					
32046654	1	41	theme	bacterial	123:131	arg1	forsythia					108:116	BACKGROUND Tannerella forsythia	86:116	BACKGROUND Tannerella forsythia	86:116	BACKGROUND Tannerella forsythia is a bacterial pathogen implicated in periodontal disease.					
32046654	2	42	from	knowledge	286:294	arg1	structure					321:329	T. forsythia's genome structure	299:329	T. forsythia's genome structure	299:329	Numerous virulence-associated T. forsythia genes have been described, however, it is necessary to expand the knowledge on T. forsythia's genome structure and genetic repertoire to further elucidate its role within pathogenesis.					
32046654	2	42	from	knowledge	286:294	arg1	repertoire					343:352	genetic repertoire	335:352	genetic repertoire	335:352	Numerous virulence-associated T. forsythia genes have been described, however, it is necessary to expand the knowledge on T. forsythia's genome structure and genetic repertoire to further elucidate its role within pathogenesis.					
32046654	10	43	theme	candidate	1350:1358	arg1	regions					1360:1366	38 candidate regions	1347:1366	38 candidate regions	1347:1366	Searching for pathogenicity islands we detected 38 candidate regions in the T. forsythia genome.					
32046654	8	44	theme	virulence	1100:1108	arg1	factors					1110:1116	known and hypothetical virulence factors	1077:1116	known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp	1077:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	18	45	theme	gene	2196:2199	arg1	loci					2201:2204	gene loci	2196:2204	gene loci that should be addressed in the context of elucidating T. forsythia's protein O-glycosylation pathway	2196:2306	Further, we report on gene loci that should be addressed in the context of elucidating T. forsythia's protein O-glycosylation pathway.					
32046654	17	46	theme	virulence	2084:2092	arg1	factors					2094:2100	T. forsythia virulence factors	2071:2100	T. forsythia virulence factors	2071:2100	Comparative analyses enabled us to assess the suitability of T. forsythia virulence factors as therapeutic targets and to suggest novel putative virulence factors.					
32046654	14	47	from	analysis	1807:1814	arg1	forsythia					1844:1852	T. forsythia	1841:1852	T. forsythia	1841:1852	Lastly, we performed a comparative analysis of translational bias in T. forsythia and Tannerella sp.					
32046654	14	47	from	analysis	1807:1814	arg1	sp					1869:1870	Tannerella sp	1858:1870	Tannerella sp	1858:1870	Lastly, we performed a comparative analysis of translational bias in T. forsythia and Tannerella sp.					
32046654	0	48	theme	pathogen	55:62	arg1	forsythia					75:83	the periodontal pathogen Tannerella forsythia	39:83	the periodontal pathogen Tannerella forsythia	39:83	Comparative genome characterization of the periodontal pathogen Tannerella forsythia.					
32046654	12	49	theme	forsythia	1544:1552	arg1	ATCC					1554:1557	T. forsythia ATCC 43037	1541:1563	T. forsythia ATCC 43037	1541:1563	While the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037 has been described previously, genes required for the initiation of glycan synthesis are yet to be discovered.					
32046654	16	50	theme	strains	2001:2007	arg1	genomes					1979:1985	the genomes	1975:1985	the genomes of Tannerella strains	1975:2007	CONCLUSIONS We provide resources and important information on the genomes of Tannerella strains.					
32046654	16	51	dep	CONCLUSIONS	1913:1923	arg1	provide					1928:1934	provide	1928:1934	provide resources and important information on the genomes of Tannerella strains	1928:2007	CONCLUSIONS We provide resources and important information on the genomes of Tannerella strains.					
32046654	13	52	theme	putative	1689:1696	arg1	loci					1712:1715	six putative glycosylation loci	1685:1715	six putative glycosylation loci which were only partially conserved in other bacteria	1685:1769	We found six putative glycosylation loci which were only partially conserved in other bacteria.					
32046654	6	53	theme	genome	798:803	arg1	assembly					805:812	a substantially improved genome assembly	773:812	a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences	773:885	RESULTS We generated a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences.					
32046654	7	54	theme	soft	955:958	arg1	genome					965:970	a soft core genome	953:970	a soft core genome encompassing 2108 genes	953:994	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	14	55	theme	bias	1833:1836	arg1	analysis					1807:1814	a comparative analysis	1793:1814	a comparative analysis of translational bias in T. forsythia and Tannerella sp	1793:1870	Lastly, we performed a comparative analysis of translational bias in T. forsythia and Tannerella sp.					
32046654	18	56	theme	O-glycosylation	2284:2298	arg1	pathway					2300:2306	T. forsythia's protein O-glycosylation pathway	2261:2306	T. forsythia's protein O-glycosylation pathway	2261:2306	Further, we report on gene loci that should be addressed in the context of elucidating T. forsythia's protein O-glycosylation pathway.					
32046654	17	57	theme	virulence	2155:2163	arg1	factors					2165:2171	novel putative virulence factors	2140:2171	novel putative virulence factors	2140:2171	Comparative analyses enabled us to assess the suitability of T. forsythia virulence factors as therapeutic targets and to suggest novel putative virulence factors.					
32046654	12	58	theme	gene	1525:1528	arg1	cluster					1530:1536	the general protein O-glycosylation gene cluster	1489:1536	the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037	1489:1563	While the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037 has been described previously, genes required for the initiation of glycan synthesis are yet to be discovered.					
32046654	19	59	theme	molecular	2356:2364	arg1	dissection					2366:2375	further molecular dissection	2348:2375	further molecular dissection of T. forsythia biology in general	2348:2410	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	11	60	theme	regions	1415:1421	arg1	four					1401:1404	four	1401:1404	four	1401:1404	Only four of these regions corresponded to previously described pathogenicity islands.					
32046654	11	60	theme	regions	1415:1421	arg1	regions					1415:1421	these regions	1409:1421	these regions	1409:1421	Only four of these regions corresponded to previously described pathogenicity islands.					
32046654	16	61	from	information	1960:1970	arg1	genomes					1979:1985	the genomes	1975:1985	the genomes of Tannerella strains	1975:2007	CONCLUSIONS We provide resources and important information on the genomes of Tannerella strains.					
32046654	7	62	theme	strains	930:936	arg1	genomes					904:910	annotated genomes	894:910	annotated genomes of ten Tannerella strains	894:936	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	14	63	theme	comparative	1795:1805	arg1	analysis					1807:1814	a comparative analysis	1793:1814	a comparative analysis of translational bias in T. forsythia and Tannerella sp	1793:1870	Lastly, we performed a comparative analysis of translational bias in T. forsythia and Tannerella sp.					
32046654	2	64	dep	T.	207:208	arg1	forsythia					210:218	forsythia	210:218	forsythia	210:218	Numerous virulence-associated T. forsythia genes have been described, however, it is necessary to expand the knowledge on T. forsythia's genome structure and genetic repertoire to further elucidate its role within pathogenesis.					
32046654	7	65	dep	established	941:951	arg1	Using					888:892	Using	888:892	Using annotated genomes of ten Tannerella strains	888:936	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	10	66	dep	detected	1338:1345	arg1	Searching					1299:1307	Searching	1299:1307	Searching for pathogenicity islands	1299:1333	Searching for pathogenicity islands we detected 38 candidate regions in the T. forsythia genome.					
32046654	8	67	theme	periodontal	1173:1183	arg1	isolate					1203:1209	the putative periodontal health-associated isolate	1160:1209	the putative periodontal health-associated isolate Tannerella sp	1160:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	4	68	theme	putative	429:436	arg1	taxon					475:479	a putative periodontal health-associated sister taxon	427:479	a putative periodontal health-associated sister taxon	427:479	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	4	68	theme	putative	429:436	arg1	available					527:535	available	527:535	available	527:535	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	11	69	theme	described	1450:1458	arg1	islands					1474:1480	previously described pathogenicity islands	1439:1480	previously described pathogenicity islands	1439:1480	Only four of these regions corresponded to previously described pathogenicity islands.					
32046654	2	70	theme	Numerous	177:184	arg1	genes					220:224	Numerous virulence-associated T. forsythia genes	177:224	Numerous virulence-associated T. forsythia genes	177:224	Numerous virulence-associated T. forsythia genes have been described, however, it is necessary to expand the knowledge on T. forsythia's genome structure and genetic repertoire to further elucidate its role within pathogenesis.					
32046654	16	71	theme	resources	1936:1944	arg1	information					1960:1970	resources and important information	1936:1970	resources and important information on the genomes of Tannerella strains	1936:2007	CONCLUSIONS We provide resources and important information on the genomes of Tannerella strains.					
32046654	14	72	from	forsythia	1844:1852	arg1	analysis					1807:1814	a comparative analysis	1793:1814	a comparative analysis of translational bias in T. forsythia and Tannerella sp	1793:1870	Lastly, we performed a comparative analysis of translational bias in T. forsythia and Tannerella sp.					
32046654	12	73	theme	protein	1501:1507	arg1	cluster					1530:1536	the general protein O-glycosylation gene cluster	1489:1536	the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037	1489:1563	While the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037 has been described previously, genes required for the initiation of glycan synthesis are yet to be discovered.					
32046654	7	74	from	present	1016:1022	arg1	%					1033:1033	> = 80%	1027:1033	> = 80% of the strains analysed	1027:1057	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	7	74	from	present	1016:1022	arg1	strains					1042:1048	the strains	1038:1048	the strains analysed	1038:1057	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	9	75	theme	candidate	1244:1252	arg1	genes					1254:1258	candidate genes	1244:1258	candidate genes promoting T. forsythia's pathogenesis	1244:1296	BU063 to identify candidate genes promoting T. forsythia's pathogenesis.					
32046654	2	76	theme	genetic	335:341	arg1	repertoire					343:352	genetic repertoire	335:352	genetic repertoire	335:352	Numerous virulence-associated T. forsythia genes have been described, however, it is necessary to expand the knowledge on T. forsythia's genome structure and genetic repertoire to further elucidate its role within pathogenesis.					
32046654	19	77	theme	species	2434:2440	arg1	virulence					2416:2424	virulence	2416:2424	virulence of this species in particular	2416:2454	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	19	77	theme	species	2434:2440	arg1	dissection					2366:2375	further molecular dissection	2348:2375	further molecular dissection of T. forsythia biology in general	2348:2410	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	16	78	theme	important	1950:1958	arg1	information					1960:1970	resources and important information	1936:1970	resources and important information on the genomes of Tannerella strains	1936:2007	CONCLUSIONS We provide resources and important information on the genomes of Tannerella strains.					
32046654	6	79	theme	forsythia	820:828	arg1	ATCC					830:833	T. forsythia ATCC 43037	817:839	T. forsythia ATCC 43037	817:839	RESULTS We generated a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences.					
32046654	4	80	theme	closest	485:491	arg1	relative					499:506	closest known relative	485:506	closest known relative	485:506	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	18	81	theme	protein	2276:2282	arg1	pathway					2300:2306	T. forsythia's protein O-glycosylation pathway	2261:2306	T. forsythia's protein O-glycosylation pathway	2261:2306	Further, we report on gene loci that should be addressed in the context of elucidating T. forsythia's protein O-glycosylation pathway.					
32046654	7	82	theme	strains	1042:1048	arg1	%					1033:1033	> = 80%	1027:1033	> = 80% of the strains analysed	1027:1057	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	7	82	theme	strains	1042:1048	arg1	strains					1042:1048	the strains	1038:1048	the strains analysed	1038:1057	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	4	83	theme	health-associated	450:466	arg1	taxon					475:479	a putative periodontal health-associated sister taxon	427:479	a putative periodontal health-associated sister taxon	427:479	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	4	83	theme	health-associated	450:466	arg1	available					527:535	available	527:535	available	527:535	BU063, a putative periodontal health-associated sister taxon and closest known relative to T. forsythia is available for comparative analyses.					
32046654	10	84	theme	T.	1375:1376	arg1	genome					1388:1393	the T. forsythia genome	1371:1393	the T. forsythia genome	1371:1393	Searching for pathogenicity islands we detected 38 candidate regions in the T. forsythia genome.					
32046654	12	85	theme	glycan	1633:1638	arg1	synthesis					1640:1648	glycan synthesis	1633:1648	glycan synthesis	1633:1648	While the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037 has been described previously, genes required for the initiation of glycan synthesis are yet to be discovered.					
32046654	8	86	used	used	1063:1066	arg2	We					1060:1061	We	1060:1061	We	1060:1061	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	10	87	theme	pathogenicity	1313:1325	arg1	islands					1327:1333	pathogenicity islands	1313:1333	pathogenicity islands	1313:1333	Searching for pathogenicity islands we detected 38 candidate regions in the T. forsythia genome.					
32046654	14	88	from	bias	1833:1836	arg1	forsythia					1844:1852	T. forsythia	1841:1852	T. forsythia	1841:1852	Lastly, we performed a comparative analysis of translational bias in T. forsythia and Tannerella sp.					
32046654	14	88	from	bias	1833:1836	arg1	sp					1869:1870	Tannerella sp	1858:1870	Tannerella sp	1858:1870	Lastly, we performed a comparative analysis of translational bias in T. forsythia and Tannerella sp.					
32046654	7	89	theme	present	1016:1022	arg1	orthologs					1006:1014	orthologs	1006:1014	orthologs present in > = 80% of the strains analysed	1006:1057	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
32046654	8	90	dep	isolate	1203:1209	arg1	sp					1222:1223	Tannerella sp	1211:1223	the putative periodontal health-associated isolate Tannerella sp	1160:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	17	91	theme	novel	2140:2144	arg1	factors					2165:2171	novel putative virulence factors	2140:2171	novel putative virulence factors	2140:2171	Comparative analyses enabled us to assess the suitability of T. forsythia virulence factors as therapeutic targets and to suggest novel putative virulence factors.					
32046654	8	92	from	comparisons	1122:1132	arg1	strains					1148:1154	pathogenic strains	1137:1154	pathogenic strains	1137:1154	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	8	92	from	comparisons	1122:1132	arg1	isolate					1203:1209	the putative periodontal health-associated isolate	1160:1209	the putative periodontal health-associated isolate Tannerella sp	1160:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	1	93	theme	BACKGROUND	86:95	arg1	pathogen					133:140	a bacterial pathogen	121:140	a bacterial pathogen implicated in periodontal disease	121:174	BACKGROUND Tannerella forsythia is a bacterial pathogen implicated in periodontal disease.					
32046654	1	93	theme	BACKGROUND	86:95	arg1	forsythia					108:116	BACKGROUND Tannerella forsythia	86:116	BACKGROUND Tannerella forsythia	86:116	BACKGROUND Tannerella forsythia is a bacterial pathogen implicated in periodontal disease.					
32046654	6	94	from	%	852:852	arg1	sequences					877:885	three sequences	871:885	three sequences	871:885	RESULTS We generated a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences.					
32046654	0	95	theme	genome	12:17	arg1	characterization					19:34	Comparative genome characterization	0:34	Comparative genome characterization of the periodontal pathogen Tannerella forsythia.	0:84	Comparative genome characterization of the periodontal pathogen Tannerella forsythia.					
32046654	8	96	theme	known	1077:1081	arg1	factors					1110:1116	known and hypothetical virulence factors	1077:1116	known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp	1077:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	5	97	theme	T.	607:608	arg1	strain					635:640	the T. forsythia reference type strain	603:640	the T. forsythia reference type strain ATCC 43037	603:651	In the past, strain confusion involving the T. forsythia reference type strain ATCC 43037 led to discrepancies between results obtained from in silico analyses and wet-lab experimentation.					
32046654	5	98	dep	in	704:705	arg1	silico					707:712	silico	707:712	silico	707:712	In the past, strain confusion involving the T. forsythia reference type strain ATCC 43037 led to discrepancies between results obtained from in silico analyses and wet-lab experimentation.					
32046654	12	99	theme	ATCC	1554:1557	arg1	cluster					1530:1536	the general protein O-glycosylation gene cluster	1489:1536	the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037	1489:1563	While the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037 has been described previously, genes required for the initiation of glycan synthesis are yet to be discovered.					
32046654	8	100	theme	hypothetical	1087:1098	arg1	factors					1110:1116	known and hypothetical virulence factors	1077:1116	known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp	1077:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	5	101	theme	reference	620:628	arg1	strain					635:640	the T. forsythia reference type strain	603:640	the T. forsythia reference type strain ATCC 43037	603:651	In the past, strain confusion involving the T. forsythia reference type strain ATCC 43037 led to discrepancies between results obtained from in silico analyses and wet-lab experimentation.					
32046654	3	102	theme	Tannerella	405:414	arg1	sp					416:417	Tannerella sp	405:417	Tannerella sp.	405:418	Tannerella sp.					
32046654	2	103	dep	virulence-associated	186:205	arg1	T.					207:208	T.	207:208	T.	207:208	Numerous virulence-associated T. forsythia genes have been described, however, it is necessary to expand the knowledge on T. forsythia's genome structure and genetic repertoire to further elucidate its role within pathogenesis.					
32046654	0	104	theme	periodontal	43:53	arg1	forsythia					75:83	the periodontal pathogen Tannerella forsythia	39:83	the periodontal pathogen Tannerella forsythia	39:83	Comparative genome characterization of the periodontal pathogen Tannerella forsythia.					
32046654	14	105	theme	Tannerella	1858:1867	arg1	sp					1869:1870	Tannerella sp	1858:1870	Tannerella sp	1858:1870	Lastly, we performed a comparative analysis of translational bias in T. forsythia and Tannerella sp.					
32046654	8	106	theme	factors	1110:1116	arg1	set					1070:1072	a set	1068:1072	a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp	1068:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	8	106	theme	factors	1110:1116	arg1	factors					1110:1116	known and hypothetical virulence factors	1077:1116	known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp	1077:1223	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	16	107	theme	Tannerella	1990:1999	arg1	strains					2001:2007	Tannerella strains	1990:2007	Tannerella strains	1990:2007	CONCLUSIONS We provide resources and important information on the genomes of Tannerella strains.					
32046654	19	108	theme	forsythia	2383:2391	arg1	biology					2393:2399	T. forsythia biology	2380:2399	T. forsythia biology	2380:2399	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	6	109	theme	T.	817:818	arg1	ATCC					830:833	T. forsythia ATCC 43037	817:839	T. forsythia ATCC 43037	817:839	RESULTS We generated a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences.					
32046654	0	110	theme	Tannerella	64:73	arg1	forsythia					75:83	the periodontal pathogen Tannerella forsythia	39:83	the periodontal pathogen Tannerella forsythia	39:83	Comparative genome characterization of the periodontal pathogen Tannerella forsythia.					
32046654	10	111	theme	forsythia	1378:1386	arg1	genome					1388:1393	the T. forsythia genome	1371:1393	the T. forsythia genome	1371:1393	Searching for pathogenicity islands we detected 38 candidate regions in the T. forsythia genome.					
32046654	19	112	theme	biology	2393:2399	arg1	virulence					2416:2424	virulence	2416:2424	virulence of this species in particular	2416:2454	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	19	112	theme	biology	2393:2399	arg1	dissection					2366:2375	further molecular dissection	2348:2375	further molecular dissection of T. forsythia biology in general	2348:2410	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	8	113	theme	pathogenic	1137:1146	arg1	strains					1148:1154	pathogenic strains	1137:1154	pathogenic strains	1137:1154	We used a set of known and hypothetical virulence factors for comparisons in pathogenic strains and the putative periodontal health-associated isolate Tannerella sp.					
32046654	12	114	theme	T.	1541:1542	arg1	ATCC					1554:1557	T. forsythia ATCC 43037	1541:1563	T. forsythia ATCC 43037	1541:1563	While the general protein O-glycosylation gene cluster of T. forsythia ATCC 43037 has been described previously, genes required for the initiation of glycan synthesis are yet to be discovered.					
32046654	19	115	from	dissection	2366:2375	arg1	particular					2445:2454	particular	2445:2454	particular	2445:2454	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	19	115	from	dissection	2366:2375	arg1	general					2404:2410	general	2404:2410	general	2404:2410	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	13	116	theme	glycosylation	1698:1710	arg1	loci					1712:1715	six putative glycosylation loci	1685:1715	six putative glycosylation loci which were only partially conserved in other bacteria	1685:1769	We found six putative glycosylation loci which were only partially conserved in other bacteria.					
32046654	6	117	theme	improved	789:796	arg1	assembly					805:812	a substantially improved genome assembly	773:812	a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences	773:885	RESULTS We generated a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences.					
32046654	19	118	theme	T.	2380:2381	arg1	biology					2393:2399	T. forsythia biology	2380:2399	T. forsythia biology	2380:2399	In summary, our work paves the way for further molecular dissection of T. forsythia biology in general and virulence of this species in particular.					
32046654	14	119	theme	translational	1819:1831	arg1	bias					1833:1836	translational bias	1819:1836	translational bias in T. forsythia and Tannerella sp	1819:1870	Lastly, we performed a comparative analysis of translational bias in T. forsythia and Tannerella sp.					
32046654	6	120	theme	genome	861:866	arg1	%					852:852	99%	850:852	99% of the genome in three sequences	850:885	RESULTS We generated a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences.					
32046654	6	120	theme	genome	861:866	arg1	genome					861:866	the genome	857:866	the genome	857:866	RESULTS We generated a substantially improved genome assembly of T. forsythia ATCC 43037 covering 99% of the genome in three sequences.					
32046654	5	121	dep	strain	635:640	arg1	ATCC					642:645	ATCC 43037	642:651	the T. forsythia reference type strain ATCC 43037	603:651	In the past, strain confusion involving the T. forsythia reference type strain ATCC 43037 led to discrepancies between results obtained from in silico analyses and wet-lab experimentation.					
32046654	14	122	from	sp	1869:1870	arg1	analysis					1807:1814	a comparative analysis	1793:1814	a comparative analysis of translational bias in T. forsythia and Tannerella sp	1793:1870	Lastly, we performed a comparative analysis of translational bias in T. forsythia and Tannerella sp.					
32046654	7	123	theme	Tannerella	919:928	arg1	strains					930:936	ten Tannerella strains	915:936	ten Tannerella strains	915:936	Using annotated genomes of ten Tannerella strains we established a soft core genome encompassing 2108 genes, based on orthologs present in > = 80% of the strains analysed.					
34151424	6	0	theme	products	933:940	arg1	development					899:909	the development	895:909	the development of hypoallergenic egg products	895:940	Furthermore, these findings may provide new insights into the development of hypoallergenic egg products.					
34151424	1	1	from	allergen	145:152	arg1	egg					157:159	egg white	157:165	egg white	157:165	Ovomucoid (OVM), known as the major allergen in egg white, has gained increasing concerns in industrialized countries.					
34151424	2	2	dep	deglycosylation	247:261	arg1	the					243:245	the	243:245	the	243:245	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	6	3	theme	hypoallergenic	914:927	arg1	products					933:940	hypoallergenic egg products	914:940	hypoallergenic egg products	914:940	Furthermore, these findings may provide new insights into the development of hypoallergenic egg products.					
34151424	2	4	theme	random	442:447	arg1	coils					449:453	random coils	442:453	random coils	442:453	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	4	theme	random	442:447	arg1	structures					410:419	other structures	404:419	other structures (β-turn, strang, and random coils)	404:454	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	5	5	theme	OVM	814:816	arg1	allergenicity					797:809	the potential allergenicity	783:809	the potential allergenicity of OVM through glycation	783:834	Therefore, GOS and FOS, especially GOS, could be applied in the reduction of the potential allergenicity of OVM through glycation.					
34151424	0	6	theme	egg	94:96	arg1	ovomucoid					98:106	the egg ovomucoid	90:106	the egg ovomucoid	90:106	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.					
34151424	0	7	from	Effects	0:6	arg1	allergenicity					73:85	allergenicity	73:85	allergenicity	73:85	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.					
34151424	0	7	from	Effects	0:6	arg1	conformation					56:67	conformation	56:67	conformation	56:67	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.					
34151424	0	8	gly	deglycosylation	11:25	arg1	ovomucoid					98:106	the egg ovomucoid	90:106	the egg ovomucoid	90:106	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.					
34151424	8	9	theme	molecular	1248:1256	arg1	characteristics					1258:1272	the molecular characteristics	1244:1272	the molecular characteristics of OVM	1244:1279	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	4	10	theme	native	694:699	arg1	OVM					701:703	native OVM	694:703	native OVM	694:703	However, the OVM glycated by mannosan (MOS) and deglycosylated OVM exhibited higher allergenicity than native OVM.					
34151424	2	11	with	reaction	276:283	arg1	GOS					314:316	GOS	314:316	GOS	314:316	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	11	with	reaction	276:283	arg1	fructooligosaccharide					323:343	fructooligosaccharide	323:343	fructooligosaccharide (FOS)	323:349	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	11	with	reaction	276:283	arg1	galactooligosaccharide					290:311	galactooligosaccharide	290:311	galactooligosaccharide (GOS)	290:317	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	11	with	reaction	276:283	arg1	FOS					346:348	FOS	346:348	FOS	346:348	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	12	dep	structures	410:419	arg1	strang					430:435	strang	430:435	strang	430:435	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	12	dep	structures	410:419	arg1	β-turn					422:427	β-turn	422:427	β-turn	422:427	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	12	dep	structures	410:419	arg1	coils					449:453	random coils	442:453	random coils	442:453	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	12	dep	structures	410:419	arg1	structures					410:419	other structures	404:419	other structures (β-turn, strang, and random coils)	404:454	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	1	13	theme	major	139:143	arg1	allergen					145:152	the major allergen	135:152	the major allergen in egg white	135:165	Ovomucoid (OVM), known as the major allergen in egg white, has gained increasing concerns in industrialized countries.					
34151424	0	14	theme	ovomucoid	98:106	arg1	allergenicity					73:85	allergenicity	73:85	allergenicity	73:85	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.					
34151424	0	14	theme	ovomucoid	98:106	arg1	conformation					56:67	conformation	56:67	conformation	56:67	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.					
34151424	7	15	theme	OVM	1019:1021	arg1	conformation					1003:1014	conformation	1003:1014	conformation	1003:1014	PRACTICAL APPLICATION: In this study, the allergenicity and conformation of OVM treated with deglycosylation and glycation (GOS, FOS, and MOS) were investigated.					
34151424	7	15	theme	OVM	1019:1021	arg1	allergenicity					985:997	allergenicity	985:997	allergenicity	985:997	PRACTICAL APPLICATION: In this study, the allergenicity and conformation of OVM treated with deglycosylation and glycation (GOS, FOS, and MOS) were investigated.					
34151424	7	16	dep	allergenicity	985:997	arg1	the					981:983	the	981:983	the	981:983	PRACTICAL APPLICATION: In this study, the allergenicity and conformation of OVM treated with deglycosylation and glycation (GOS, FOS, and MOS) were investigated.					
34151424	8	17	with	understanding	1140:1152	arg1	sugars					1234:1239	different reducing sugars	1215:1239	different reducing sugars on the molecular characteristics of OVM	1215:1279	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	8	18	from	sugars	1234:1239	arg1	characteristics					1258:1272	the molecular characteristics	1244:1272	the molecular characteristics of OVM	1244:1279	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	4	19	theme	higher	668:673	arg1	allergenicity					675:687	higher allergenicity	668:687	higher allergenicity	668:687	However, the OVM glycated by mannosan (MOS) and deglycosylated OVM exhibited higher allergenicity than native OVM.					
34151424	8	20	theme	better	1133:1138	arg1	understanding					1140:1152	a better understanding	1131:1152	a better understanding of the effects of deglycosylation	1131:1186	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	8	21	theme	OVM	1277:1279	arg1	characteristics					1258:1272	the molecular characteristics	1244:1272	the molecular characteristics of OVM	1244:1279	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	8	22	with	reaction	1201:1208	arg1	sugars					1234:1239	different reducing sugars	1215:1239	different reducing sugars on the molecular characteristics of OVM	1215:1279	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	7	23	dep	deglycosylation	1036:1050	arg1	GOS					1067:1069	GOS	1067:1069	GOS	1067:1069	PRACTICAL APPLICATION: In this study, the allergenicity and conformation of OVM treated with deglycosylation and glycation (GOS, FOS, and MOS) were investigated.					
34151424	7	23	dep	deglycosylation	1036:1050	arg1	FOS					1072:1074	FOS	1072:1074	FOS	1072:1074	PRACTICAL APPLICATION: In this study, the allergenicity and conformation of OVM treated with deglycosylation and glycation (GOS, FOS, and MOS) were investigated.					
34151424	7	23	dep	deglycosylation	1036:1050	arg1	MOS					1081:1083	MOS	1081:1083	MOS	1081:1083	PRACTICAL APPLICATION: In this study, the allergenicity and conformation of OVM treated with deglycosylation and glycation (GOS, FOS, and MOS) were investigated.					
34151424	8	24	theme	egg	1353:1355	arg1	products					1357:1364	hypoallergenic egg products	1338:1364	hypoallergenic egg products	1338:1364	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	2	25	theme	Maillard	267:274	arg1	reaction					276:283	Maillard reaction	267:283	Maillard reaction	267:283	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	1	26	theme	white	161:165	arg1	egg					157:159	egg white	157:165	egg white	157:165	Ovomucoid (OVM), known as the major allergen in egg white, has gained increasing concerns in industrialized countries.					
34151424	2	27	theme	other	404:408	arg1	strang					430:435	strang	430:435	strang	430:435	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	27	theme	other	404:408	arg1	β-turn					422:427	β-turn	422:427	β-turn	422:427	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	27	theme	other	404:408	arg1	structures					410:419	other structures	404:419	other structures (β-turn, strang, and random coils)	404:454	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	27	theme	other	404:408	arg1	coils					449:453	random coils	442:453	random coils	442:453	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	6	28	theme	new	877:879	arg1	insights					881:888	new insights	877:888	new insights into the development of hypoallergenic egg products	877:940	Furthermore, these findings may provide new insights into the development of hypoallergenic egg products.					
34151424	2	29	theme	OVM	395:397	arg1	transformation					377:390	conformational transformation	362:390	conformational transformation of OVM from other structures (β-turn, strang, and random coils)	362:454	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	0	30	theme	Maillard	35:42	arg1	reaction					44:51	the Maillard reaction	31:51	the Maillard reaction	31:51	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.					
34151424	4	31	gly	deglycosylated	639:652	arg1	OVM					654:656	deglycosylated OVM	639:656	deglycosylated OVM	639:656	However, the OVM glycated by mannosan (MOS) and deglycosylated OVM exhibited higher allergenicity than native OVM.					
34151424	8	32	theme	products	1357:1364	arg1	development					1323:1333	the development	1319:1333	the development of hypoallergenic egg products	1319:1364	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	3	33	theme	OVM	548:550	arg1	allergenicity					513:525	the allergenicity	509:525	the allergenicity of Gallus domesticus OVM	509:550	We also introduced an approach to reduce the allergenicity of Gallus domesticus OVM by Maillard reaction with GOS and FOS.					
34151424	8	34	theme	deglycosylation	1172:1186	arg1	effects					1161:1167	the effects	1157:1167	the effects of deglycosylation	1157:1186	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	2	35	theme	conformational	362:375	arg1	transformation					377:390	conformational transformation	362:390	conformational transformation of OVM from other structures (β-turn, strang, and random coils)	362:454	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	8	36	theme	different	1215:1223	arg1	sugars					1234:1239	different reducing sugars	1215:1239	different reducing sugars on the molecular characteristics of OVM	1215:1279	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	6	37	theme	egg	929:931	arg1	products					933:940	hypoallergenic egg products	914:940	hypoallergenic egg products	914:940	Furthermore, these findings may provide new insights into the development of hypoallergenic egg products.					
34151424	7	38	dep	APPLICATION	953:963	arg1	investigated					1091:1102	investigated	1091:1102	were investigated	1086:1102	PRACTICAL APPLICATION: In this study, the allergenicity and conformation of OVM treated with deglycosylation and glycation (GOS, FOS, and MOS) were investigated.					
34151424	3	39	theme	Gallus	530:535	arg1	OVM					548:550	Gallus domesticus OVM	530:550	Gallus domesticus OVM	530:550	We also introduced an approach to reduce the allergenicity of Gallus domesticus OVM by Maillard reaction with GOS and FOS.					
34151424	7	40	theme	PRACTICAL	943:951	arg1	APPLICATION					953:963	PRACTICAL APPLICATION	943:963	PRACTICAL APPLICATION: In this study, the allergenicity and conformation of OVM treated with deglycosylation and glycation (GOS, FOS, and MOS) were investigated.	943:1103	PRACTICAL APPLICATION: In this study, the allergenicity and conformation of OVM treated with deglycosylation and glycation (GOS, FOS, and MOS) were investigated.					
34151424	8	41	theme	Maillard	1192:1199	arg1	reaction					1201:1208	Maillard reaction	1192:1208	Maillard reaction with different reducing sugars on the molecular characteristics of OVM	1192:1279	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	0	42	theme	reaction	44:51	arg1	Effects					0:6	Effects	0:6	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid	0:106	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.					
34151424	3	43	theme	domesticus	537:546	arg1	OVM					548:550	Gallus domesticus OVM	530:550	Gallus domesticus OVM	530:550	We also introduced an approach to reduce the allergenicity of Gallus domesticus OVM by Maillard reaction with GOS and FOS.					
34151424	8	44	theme	hypoallergenic	1338:1351	arg1	products					1357:1364	hypoallergenic egg products	1338:1364	hypoallergenic egg products	1338:1364	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	8	45	theme	new	1301:1303	arg1	insights					1305:1312	new insights	1301:1312	new insights into the development of hypoallergenic egg products	1301:1364	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	8	46	theme	reducing	1225:1232	arg1	sugars					1234:1239	different reducing sugars	1215:1239	different reducing sugars on the molecular characteristics of OVM	1215:1279	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	4	47	theme	deglycosylated	639:652	arg1	OVM					654:656	deglycosylated OVM	639:656	deglycosylated OVM	639:656	However, the OVM glycated by mannosan (MOS) and deglycosylated OVM exhibited higher allergenicity than native OVM.					
34151424	3	48	theme	Maillard	555:562	arg1	reaction					564:571	Maillard reaction	555:571	Maillard reaction with GOS and FOS	555:588	We also introduced an approach to reduce the allergenicity of Gallus domesticus OVM by Maillard reaction with GOS and FOS.					
34151424	8	49	theme	effects	1161:1167	arg1	understanding					1140:1152	a better understanding	1131:1152	a better understanding of the effects of deglycosylation	1131:1186	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	8	49	theme	effects	1161:1167	arg1	reaction					1201:1208	Maillard reaction	1192:1208	Maillard reaction with different reducing sugars on the molecular characteristics of OVM	1192:1279	The results would provide a better understanding of the effects of deglycosylation and Maillard reaction with different reducing sugars on the molecular characteristics of OVM and further provide new insights into the development of hypoallergenic egg products.					
34151424	5	50	theme	potential	787:795	arg1	allergenicity					797:809	the potential allergenicity	783:809	the potential allergenicity of OVM through glycation	783:834	Therefore, GOS and FOS, especially GOS, could be applied in the reduction of the potential allergenicity of OVM through glycation.					
34151424	2	51	with	deglycosylation	247:261	arg1	GOS					314:316	GOS	314:316	GOS	314:316	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	51	with	deglycosylation	247:261	arg1	fructooligosaccharide					323:343	fructooligosaccharide	323:343	fructooligosaccharide (FOS)	323:349	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	51	with	deglycosylation	247:261	arg1	galactooligosaccharide					290:311	galactooligosaccharide	290:311	galactooligosaccharide (GOS)	290:317	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	2	51	with	deglycosylation	247:261	arg1	FOS					346:348	FOS	346:348	FOS	346:348	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	3	52	with	reaction	564:571	arg1	FOS					586:588	FOS	586:588	FOS	586:588	We also introduced an approach to reduce the allergenicity of Gallus domesticus OVM by Maillard reaction with GOS and FOS.					
34151424	3	52	with	reaction	564:571	arg1	GOS					578:580	GOS	578:580	GOS	578:580	We also introduced an approach to reduce the allergenicity of Gallus domesticus OVM by Maillard reaction with GOS and FOS.					
34151424	2	53	from	structures	410:419	arg1	transformation					377:390	conformational transformation	362:390	conformational transformation of OVM from other structures (β-turn, strang, and random coils)	362:454	Here, we found the deglycosylation and Maillard reaction with galactooligosaccharide (GOS) and fructooligosaccharide (FOS) can induce conformational transformation of OVM from other structures (β-turn, strang, and random coils) to α-helix.					
34151424	0	54	theme	deglycosylation	11:25	arg1	Effects					0:6	Effects	0:6	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid	0:106	Effects of deglycosylation and the Maillard reaction on conformation and allergenicity of the egg ovomucoid.					
34151424	5	55	theme	allergenicity	797:809	arg1	reduction					770:778	the reduction	766:778	the reduction of the potential allergenicity of OVM through glycation	766:834	Therefore, GOS and FOS, especially GOS, could be applied in the reduction of the potential allergenicity of OVM through glycation.					
34151424	1	56	theme	industrialized	202:215	arg1	countries					217:225	industrialized countries	202:225	industrialized countries	202:225	Ovomucoid (OVM), known as the major allergen in egg white, has gained increasing concerns in industrialized countries.					
34192306	5	0	theme	wall	934:937	arg1	changes					953:959	cell wall compositional changes	929:959	cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration	929:1121	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	2	1	theme	grape	350:354	arg1	industries					356:365	the table and wine grape industries	331:365	industries	356:365	Damage caused by this pathogen is severely detrimental to the table and wine grape industries and results in substantial crop losses worldwide.					
34192306	9	2	theme	corresponding	1679:1691	arg1	depolymerization					1700:1715	corresponding pectin depolymerization	1679:1715	corresponding pectin depolymerization	1679:1715	The table grape cultivars showed more severe infection symptoms, and corresponding pectin depolymerization, compared with wine grape cultivars.					
34192306	6	3	theme	computed	1203:1210	arg1	CT					1224:1225	CT	1224:1225	CT	1224:1225	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	6	3	theme	computed	1203:1210	arg1	tomography					1212:1221	X-ray computed tomography	1197:1221	X-ray computed tomography (CT)	1197:1226	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	10	4	located	present	1973:1979	arg2	evidence					1929:1936	evidence	1929:1936	evidence of extensin epitope deposition	1929:1967	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	10	4	located	present	1973:1979	arg1	moderate					1876:1883	moderate	1876:1883	moderate	1876:1883	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	12	5	theme	important	2131:2139	arg1	factors					2123:2129	Cell wall factors	2113:2129	Cell wall factors important in influencing resistance	2113:2165	Cell wall factors important in influencing resistance may include pectin methylesterification profiles, as well as extensin reorganization.					
34192306	7	6	theme	degradation	1452:1462	arg1	evidence					1421:1428	evidence	1421:1428	evidence of significant tissue degradation	1421:1462	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	3	7	theme	defence-related	543:557	arg1	molecules					569:577	some defence-related messenger molecules	538:577	some defence-related messenger molecules	538:577	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	5	8	theme	first	903:907	arg1	time					909:912	the first time	899:912	the first time	899:912	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	7	9	theme	significant	1433:1443	arg1	degradation					1452:1462	significant tissue degradation	1433:1462	significant tissue degradation	1433:1462	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	11	10	theme	disease	2089:2095	arg1	susceptibility					2097:2110	fungal disease susceptibility	2082:2110	fungal disease susceptibility	2082:2110	CONCLUSIONS Specific changes in the grape cell wall compositional profiles appear to correlate with fungal disease susceptibility.					
34192306	1	11	theme	broad	187:191	arg1	cultivars					262:270	domesticated grapevine Vitis vinifera cultivars	224:270	domesticated grapevine Vitis vinifera cultivars	224:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	1	11	theme	broad	187:191	arg1	range					193:197	a broad range	185:197	a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars	185:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	9	12	theme	wine	1732:1735	arg1	cultivars					1743:1751	wine grape cultivars	1732:1751	wine grape cultivars	1732:1751	The table grape cultivars showed more severe infection symptoms, and corresponding pectin depolymerization, compared with wine grape cultivars.					
34192306	5	13	with	combination	828:838	arg1	analysis					874:881	monosaccharide compositional analysis	845:881	monosaccharide compositional analysis	845:881	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	12	14	theme	pectin	2179:2184	arg1	profiles					2207:2214	pectin methylesterification profiles	2179:2214	pectin methylesterification profiles	2179:2214	Cell wall factors important in influencing resistance may include pectin methylesterification profiles, as well as extensin reorganization.					
34192306	5	15	theme	tissue	1105:1110	arg1	maceration					1112:1121	tissue maceration	1105:1121	tissue maceration	1105:1121	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	8	16	from	epitopes	1531:1538	arg1	walls					1558:1562	the berry cell walls	1543:1562	the berry cell walls	1543:1562	The latter was characterized by a reduction in signals for pectin epitopes in the berry cell walls, implying the degradation of pectin polymers.					
34192306	1	17	theme	crops	208:212	arg1	cultivars					262:270	domesticated grapevine Vitis vinifera cultivars	224:270	domesticated grapevine Vitis vinifera cultivars	224:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	1	17	theme	crops	208:212	arg1	range					193:197	a broad range	185:197	a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars	185:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	5	18	dep	METHODS	759:765	arg1	applied					887:893	applied	887:893	was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration	883:1121	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	10	19	dep	unaffected	1809:1818	arg1	whereas					1865:1871	whereas	1865:1871	whereas	1865:1871	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	5	20	theme	grape	1059:1063	arg1	Barlinka					1049:1056	Barlinka	1049:1056	Barlinka	1049:1056	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	5	20	theme	grape	1059:1063	arg1	cultivars					1065:1073	wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars	979:1073	cultivars	1065:1073	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	5	20	theme	grape	1059:1063	arg1	Dauphine					1036:1043	Dauphine	1036:1043	Dauphine	1036:1043	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	11	21	theme	Specific	1994:2001	arg1	changes					2003:2009	CONCLUSIONS Specific changes	1982:2009	CONCLUSIONS Specific changes in the grape cell wall compositional profiles	1982:2055	CONCLUSIONS Specific changes in the grape cell wall compositional profiles appear to correlate with fungal disease susceptibility.					
34192306	4	22	theme	fruit	746:750	arg1	crops					752:756	other fruit crops	740:756	other fruit crops	740:756	Limited studies have investigated changes in grape cell wall composition upon infection with B. cinerea, with much being inferred from studies on other fruit crops.					
34192306	11	23	theme	wall	2029:2032	arg1	profiles					2048:2055	the grape cell wall compositional profiles	2014:2055	the grape cell wall compositional profiles	2014:2055	CONCLUSIONS Specific changes in the grape cell wall compositional profiles appear to correlate with fungal disease susceptibility.					
34192306	5	24	from	profiling	815:823	arg1	combination					828:838	combination	828:838	combination with monosaccharide compositional analysis	828:881	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	5	25	theme	compositional	860:872	arg1	analysis					874:881	monosaccharide compositional analysis	845:881	monosaccharide compositional analysis	845:881	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	5	26	theme	cell	929:932	arg1	changes					953:959	cell wall compositional changes	929:959	cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration	929:1121	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	10	27	from	unaffected	1809:1818	arg1	types					1768:1772	both grape types	1757:1772	both grape types	1757:1772	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	8	28	theme	pectin	1524:1529	arg1	epitopes					1531:1538	pectin epitopes	1524:1538	pectin epitopes in the berry cell walls	1524:1562	The latter was characterized by a reduction in signals for pectin epitopes in the berry cell walls, implying the degradation of pectin polymers.					
34192306	12	29	theme	wall	2118:2121	arg1	factors					2123:2129	Cell wall factors	2113:2129	Cell wall factors important in influencing resistance	2113:2165	Cell wall factors important in influencing resistance may include pectin methylesterification profiles, as well as extensin reorganization.					
34192306	0	30	theme	Botrytis	63:70	arg1	infection					80:88	Botrytis cinerea infection	63:88	Botrytis cinerea infection using glycan microarrays	63:113	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.					
34192306	1	31	theme	Botrytis	160:167	arg1	cinerea					169:175	The necrotrophic fungus Botrytis cinerea	136:175	The necrotrophic fungus Botrytis cinerea	136:175	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	12	32	theme	methylesterification	2186:2205	arg1	profiles					2207:2214	pectin methylesterification profiles	2179:2214	pectin methylesterification profiles	2179:2214	Cell wall factors important in influencing resistance may include pectin methylesterification profiles, as well as extensin reorganization.					
34192306	10	33	dep	content	1856:1862	arg1	was					1824:1826	was	1824:1826	was	1824:1826	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	10	33	dep	content	1856:1862	arg1	as					1821:1822	as	1821:1822	as	1821:1822	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	8	34	theme	berry	1547:1551	arg1	walls					1558:1562	the berry cell walls	1543:1562	the berry cell walls	1543:1562	The latter was characterized by a reduction in signals for pectin epitopes in the berry cell walls, implying the degradation of pectin polymers.					
34192306	2	35	theme	losses	399:404	arg1	worldwide					406:414	substantial crop losses worldwide	382:414	substantial crop losses worldwide	382:414	Damage caused by this pathogen is severely detrimental to the table and wine grape industries and results in substantial crop losses worldwide.					
34192306	0	36	theme	glycan	96:101	arg1	microarrays					103:113	glycan microarrays	96:113	glycan microarrays	96:113	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.					
34192306	5	37	theme	compositional	939:951	arg1	changes					953:959	cell wall compositional changes	929:959	cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration	929:1121	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	0	38	theme	wine	30:33	arg1	grapes					45:50	wine and table grapes	30:50	wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays	30:113	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.					
34192306	2	39	theme	substantial	382:392	arg1	losses					399:404	substantial crop losses	382:404	substantial crop losses worldwide	382:414	Damage caused by this pathogen is severely detrimental to the table and wine grape industries and results in substantial crop losses worldwide.					
34192306	0	40	theme	Tracking	0:7	arg1	changes					19:25	Tracking cell wall changes	0:25	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.	0:114	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.					
34192306	5	41	from	changes	953:959	arg1	berries					968:974	the berries	964:974	the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration	964:1121	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	5	42	theme	wine	979:982	arg1	berries					968:974	the berries	964:974	the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration	964:1121	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	3	43	theme	important	460:468	arg1	interface					444:452	The apoplast and cell wall interface	417:452	interface	444:452	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	3	43	theme	important	460:468	arg1	apoplast					421:428	The apoplast and cell wall interface	417:452	apoplast	421:428	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	3	43	theme	important	460:468	arg1	setting					470:476	an important setting	457:476	an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated	457:591	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	0	44	theme	wall	14:17	arg1	changes					19:25	Tracking cell wall changes	0:25	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.	0:114	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.					
34192306	11	45	theme	compositional	2034:2046	arg1	profiles					2048:2055	the grape cell wall compositional profiles	2014:2055	the grape cell wall compositional profiles	2014:2055	CONCLUSIONS Specific changes in the grape cell wall compositional profiles appear to correlate with fungal disease susceptibility.					
34192306	7	46	theme	infection	1331:1339	arg1	symptoms					1341:1348	visible infection symptoms	1323:1348	visible infection symptoms	1323:1348	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	5	47	theme	polymer	807:813	arg1	profiling					815:823	comprehensive microarray polymer profiling	782:823	comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis	782:881	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	9	48	theme	grape	1620:1624	arg1	cultivars					1626:1634	The table grape cultivars	1610:1634	The table grape cultivars	1610:1634	The table grape cultivars showed more severe infection symptoms, and corresponding pectin depolymerization, compared with wine grape cultivars.					
34192306	8	49	theme	pectin	1593:1598	arg1	polymers					1600:1607	pectin polymers	1593:1607	pectin polymers	1593:1607	The latter was characterized by a reduction in signals for pectin epitopes in the berry cell walls, implying the degradation of pectin polymers.					
34192306	3	50	theme	plant-pathogen	489:502	arg1	interactions					504:515	many plant-pathogen interactions	484:515	many plant-pathogen interactions	484:515	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	11	51	theme	grape	2018:2022	arg1	profiles					2048:2055	the grape cell wall compositional profiles	2014:2055	the grape cell wall compositional profiles	2014:2055	CONCLUSIONS Specific changes in the grape cell wall compositional profiles appear to correlate with fungal disease susceptibility.					
34192306	4	52	with	infection	672:680	arg1	cinerea					690:696	B. cinerea	687:696	B. cinerea	687:696	Limited studies have investigated changes in grape cell wall composition upon infection with B. cinerea, with much being inferred from studies on other fruit crops.					
34192306	9	53	theme	severe	1648:1653	arg1	symptoms					1665:1672	more severe infection symptoms	1643:1672	more severe infection symptoms	1643:1672	The table grape cultivars showed more severe infection symptoms, and corresponding pectin depolymerization, compared with wine grape cultivars.					
34192306	1	54	theme	necrotrophic	140:151	arg1	cinerea					169:175	The necrotrophic fungus Botrytis cinerea	136:175	The necrotrophic fungus Botrytis cinerea	136:175	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	10	55	theme	table	1906:1910	arg1	cultivars					1918:1926	severely infected table grape cultivars	1888:1926	severely infected table grape cultivars	1888:1926	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	4	56	from	studies	729:735	arg1	crops					752:756	other fruit crops	740:756	other fruit crops	740:756	Limited studies have investigated changes in grape cell wall composition upon infection with B. cinerea, with much being inferred from studies on other fruit crops.					
34192306	1	57	theme	grapevine	237:245	arg1	vinifera					253:260	domesticated grapevine Vitis vinifera	224:260	domesticated grapevine Vitis vinifera cultivars	224:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	10	58	from	moderate	1876:1883	arg1	present					1973:1979	present	1973:1979	present	1973:1979	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	6	59	with	conjunction	1141:1151	arg1	CT					1224:1225	CT	1224:1225	CT	1224:1225	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	6	59	with	conjunction	1141:1151	arg1	tomography					1212:1221	X-ray computed tomography	1197:1221	X-ray computed tomography (CT)	1197:1226	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	6	59	with	conjunction	1141:1151	arg1	SEM					1188:1190	SEM	1188:1190	SEM	1188:1190	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	6	59	with	conjunction	1141:1151	arg1	microscopy					1176:1185	scanning electron microscopy	1158:1185	scanning electron microscopy (SEM)	1158:1191	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	3	60	dep	setting	470:476	arg1	generated					583:591	generated	583:591	are generated	579:591	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	3	60	dep	setting	470:476	arg1	take					517:520	take	517:520	take place	517:526	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	12	61	theme	Cell	2113:2116	arg1	factors					2123:2129	Cell wall factors	2113:2129	Cell wall factors important in influencing resistance	2113:2165	Cell wall factors important in influencing resistance may include pectin methylesterification profiles, as well as extensin reorganization.					
34192306	4	62	theme	cell	645:648	arg1	composition					655:665	grape cell wall composition	639:665	grape cell wall composition	639:665	Limited studies have investigated changes in grape cell wall composition upon infection with B. cinerea, with much being inferred from studies on other fruit crops.					
34192306	10	63	theme	extensin	1941:1948	arg1	deposition					1958:1967	extensin epitope deposition	1941:1967	extensin epitope deposition	1941:1967	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	10	64	theme	deposition	1958:1967	arg1	evidence					1929:1936	evidence	1929:1936	evidence of extensin epitope deposition	1929:1967	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	1	65	theme	vinifera	253:260	arg1	cultivars					262:270	domesticated grapevine Vitis vinifera cultivars	224:270	domesticated grapevine Vitis vinifera cultivars	224:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	2	66	from	table	335:339	arg1	worldwide					406:414	substantial crop losses worldwide	382:414	substantial crop losses worldwide	382:414	Damage caused by this pathogen is severely detrimental to the table and wine grape industries and results in substantial crop losses worldwide.					
34192306	7	67	theme	KEY	1267:1269	arg1	RESULTS					1271:1277	KEY RESULTS	1267:1277	KEY RESULTS Grapes infected at veraison	1267:1305	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	7	67	theme	KEY	1267:1269	arg1	Grapes					1279:1284	Grapes	1279:1284	Grapes	1279:1284	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	3	68	theme	messenger	559:567	arg1	molecules					569:577	some defence-related messenger molecules	538:577	some defence-related messenger molecules	538:577	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	9	69	theme	pectin	1693:1698	arg1	depolymerization					1700:1715	corresponding pectin depolymerization	1679:1715	corresponding pectin depolymerization	1679:1715	The table grape cultivars showed more severe infection symptoms, and corresponding pectin depolymerization, compared with wine grape cultivars.					
34192306	6	70	theme	X-ray	1197:1201	arg1	CT					1224:1225	CT	1224:1225	CT	1224:1225	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	6	70	theme	X-ray	1197:1201	arg1	tomography					1212:1221	X-ray computed tomography	1197:1221	X-ray computed tomography (CT)	1197:1226	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	5	71	dep	cultivars	1065:1073	arg1	Barlinka					1049:1056	Barlinka	1049:1056	Barlinka	1049:1056	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	5	71	dep	cultivars	1065:1073	arg1	cultivars					1065:1073	wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars	979:1073	cultivars	1065:1073	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	5	71	dep	cultivars	1065:1073	arg1	Dauphine					1036:1043	Dauphine	1036:1043	Dauphine	1036:1043	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	2	72	theme	wine	345:348	arg1	industries					356:365	the table and wine grape industries	331:365	industries	356:365	Damage caused by this pathogen is severely detrimental to the table and wine grape industries and results in substantial crop losses worldwide.					
34192306	10	73	theme	hemicellulose	1775:1787	arg1	layers					1789:1794	hemicellulose layers	1775:1794	hemicellulose layers	1775:1794	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	2	74	from	industries	356:365	arg1	worldwide					406:414	substantial crop losses worldwide	382:414	substantial crop losses worldwide	382:414	Damage caused by this pathogen is severely detrimental to the table and wine grape industries and results in substantial crop losses worldwide.					
34192306	4	75	theme	B.	687:688	arg1	cinerea					690:696	B. cinerea	687:696	B. cinerea	687:696	Limited studies have investigated changes in grape cell wall composition upon infection with B. cinerea, with much being inferred from studies on other fruit crops.					
34192306	5	76	theme	comprehensive	782:794	arg1	profiling					815:823	comprehensive microarray polymer profiling	782:823	comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis	782:881	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	11	77	theme	fungal	2082:2087	arg1	susceptibility					2097:2110	fungal disease susceptibility	2082:2110	fungal disease susceptibility	2082:2110	CONCLUSIONS Specific changes in the grape cell wall compositional profiles appear to correlate with fungal disease susceptibility.					
34192306	2	78	from	results	371:377	arg1	worldwide					406:414	substantial crop losses worldwide	382:414	substantial crop losses worldwide	382:414	Damage caused by this pathogen is severely detrimental to the table and wine grape industries and results in substantial crop losses worldwide.					
34192306	7	79	theme	tissue	1445:1450	arg1	degradation					1452:1462	significant tissue degradation	1433:1462	significant tissue degradation	1433:1462	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	10	80	from	types	1768:1772	arg1	unaffected					1809:1818	unaffected	1809:1818	unaffected	1809:1818	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	9	81	theme	grape	1737:1741	arg1	cultivars					1743:1751	wine grape cultivars	1732:1751	wine grape cultivars	1732:1751	The table grape cultivars showed more severe infection symptoms, and corresponding pectin depolymerization, compared with wine grape cultivars.					
34192306	6	82	theme	electron	1167:1174	arg1	SEM					1188:1190	SEM	1188:1190	SEM	1188:1190	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	6	82	theme	electron	1167:1174	arg1	microscopy					1176:1185	scanning electron microscopy	1158:1185	scanning electron microscopy (SEM)	1158:1191	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	11	83	from	changes	2003:2009	arg1	profiles					2048:2055	the grape cell wall compositional profiles	2014:2055	the grape cell wall compositional profiles	2014:2055	CONCLUSIONS Specific changes in the grape cell wall compositional profiles appear to correlate with fungal disease susceptibility.					
34192306	10	84	theme	protein	1848:1854	arg1	content					1856:1862	the arabinogalactan protein content	1828:1862	the arabinogalactan protein content	1828:1862	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	1	85	theme	fruit	202:206	arg1	cultivars					262:270	domesticated grapevine Vitis vinifera cultivars	224:270	domesticated grapevine Vitis vinifera cultivars	224:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	1	85	theme	fruit	202:206	arg1	crops					208:212	fruit crops	202:212	fruit crops including domesticated grapevine Vitis vinifera cultivars	202:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	6	86	theme	infection	1244:1252	arg1	progression					1254:1264	infection progression	1244:1264	infection progression	1244:1264	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	11	87	dep	Specific	1994:2001	arg1	CONCLUSIONS					1982:1992	CONCLUSIONS	1982:1992	CONCLUSIONS	1982:1992	CONCLUSIONS Specific changes in the grape cell wall compositional profiles appear to correlate with fungal disease susceptibility.					
34192306	10	88	from	present	1973:1979	arg1	moderate					1876:1883	moderate	1876:1883	moderate	1876:1883	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	7	89	theme	ripe	1402:1405	arg1	stages					1407:1412	the post-veraison and ripe stages	1380:1412	the post-veraison and ripe stages	1380:1412	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	5	90	theme	cultivars	1065:1073	arg1	berries					968:974	the berries	964:974	the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration	964:1121	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	11	91	theme	cell	2024:2027	arg1	profiles					2048:2055	the grape cell wall compositional profiles	2014:2055	the grape cell wall compositional profiles	2014:2055	CONCLUSIONS Specific changes in the grape cell wall compositional profiles appear to correlate with fungal disease susceptibility.					
34192306	6	92	used	used	1133:1136	arg2	This					1124:1127	This	1124:1127	This	1124:1127	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	7	93	theme	post-veraison	1384:1396	arg1	stages					1407:1412	the post-veraison and ripe stages	1380:1412	the post-veraison and ripe stages	1380:1412	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	4	94	theme	other	740:744	arg1	crops					752:756	other fruit crops	740:756	other fruit crops	740:756	Limited studies have investigated changes in grape cell wall composition upon infection with B. cinerea, with much being inferred from studies on other fruit crops.					
34192306	5	95	theme	Botrytis	1082:1089	arg1	infection					1091:1099	Botrytis infection	1082:1099	Botrytis infection	1082:1099	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	0	96	from	changes	19:25	arg1	grapes					45:50	wine and table grapes	30:50	wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays	30:113	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.					
34192306	0	97	theme	cinerea	72:78	arg1	infection					80:88	Botrytis cinerea infection	63:88	Botrytis cinerea infection using glycan microarrays	63:113	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.					
34192306	1	98	theme	fungus	153:158	arg1	cinerea					169:175	The necrotrophic fungus Botrytis cinerea	136:175	The necrotrophic fungus Botrytis cinerea	136:175	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	5	99	dep	wine	979:982	arg1	Blanc					995:999	Blanc	995:999	Blanc	995:999	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	5	99	dep	wine	979:982	arg1	Sauvignon					1014:1022	Sauvignon	1014:1022	Sauvignon	1014:1022	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	4	100	from	changes	628:634	arg1	composition					655:665	grape cell wall composition	639:665	grape cell wall composition	639:665	Limited studies have investigated changes in grape cell wall composition upon infection with B. cinerea, with much being inferred from studies on other fruit crops.					
34192306	7	101	dep	infected	1286:1293	arg1	RESULTS					1271:1277	KEY RESULTS	1267:1277	KEY RESULTS Grapes infected at veraison	1267:1305	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	7	101	dep	infected	1286:1293	arg1	Grapes					1279:1284	Grapes	1279:1284	Grapes	1279:1284	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	8	102	theme	cell	1553:1556	arg1	walls					1558:1562	the berry cell walls	1543:1562	the berry cell walls	1543:1562	The latter was characterized by a reduction in signals for pectin epitopes in the berry cell walls, implying the degradation of pectin polymers.					
34192306	10	103	attach	present	1973:1979	arg2	evidence					1929:1936	evidence	1929:1936	evidence of extensin epitope deposition	1929:1967	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	10	103	attach	present	1973:1979	arg1	moderate					1876:1883	moderate	1876:1883	moderate	1876:1883	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	1	104	dep	BACKGROUND	116:125	arg1	infects					177:183	infects	177:183	infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars	177:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	2	105	theme	crop	394:397	arg1	losses					399:404	substantial crop losses	382:404	substantial crop losses worldwide	382:414	Damage caused by this pathogen is severely detrimental to the table and wine grape industries and results in substantial crop losses worldwide.					
34192306	0	106	theme	cell	9:12	arg1	changes					19:25	Tracking cell wall changes	0:25	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.	0:114	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.					
34192306	3	107	theme	cell	434:437	arg1	interface					444:452	The apoplast and cell wall interface	417:452	interface	444:452	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	3	107	theme	cell	434:437	arg1	apoplast					421:428	The apoplast and cell wall interface	417:452	apoplast	421:428	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	3	107	theme	cell	434:437	arg1	setting					470:476	an important setting	457:476	an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated	457:591	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	5	108	theme	monosaccharide	845:858	arg1	analysis					874:881	monosaccharide compositional analysis	845:881	monosaccharide compositional analysis	845:881	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	9	109	theme	infection	1655:1663	arg1	symptoms					1665:1672	more severe infection symptoms	1643:1672	more severe infection symptoms	1643:1672	The table grape cultivars showed more severe infection symptoms, and corresponding pectin depolymerization, compared with wine grape cultivars.					
34192306	7	110	theme	visible	1323:1329	arg1	symptoms					1341:1348	visible infection symptoms	1323:1348	visible infection symptoms	1323:1348	KEY RESULTS Grapes infected at veraison did not develop visible infection symptoms, whereas grapes inoculated at the post-veraison and ripe stages showed evidence of significant tissue degradation.					
34192306	4	111	theme	Limited	594:600	arg1	studies					602:608	Limited studies	594:608	Limited studies	594:608	Limited studies have investigated changes in grape cell wall composition upon infection with B. cinerea, with much being inferred from studies on other fruit crops.					
34192306	10	112	theme	infected	1897:1904	arg1	cultivars					1918:1926	severely infected table grape cultivars	1888:1926	severely infected table grape cultivars	1888:1926	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	5	113	theme	microarray	796:805	arg1	profiling					815:823	comprehensive microarray polymer profiling	782:823	comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis	782:881	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	9	114	theme	table	1614:1618	arg1	cultivars					1626:1634	The table grape cultivars	1610:1634	The table grape cultivars	1610:1634	The table grape cultivars showed more severe infection symptoms, and corresponding pectin depolymerization, compared with wine grape cultivars.					
34192306	3	115	theme	wall	439:442	arg1	interface					444:452	The apoplast and cell wall interface	417:452	interface	444:452	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	3	115	theme	wall	439:442	arg1	apoplast					421:428	The apoplast and cell wall interface	417:452	apoplast	421:428	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	3	115	theme	wall	439:442	arg1	setting					470:476	an important setting	457:476	an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated	457:591	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	8	116	theme	polymers	1600:1607	arg1	degradation					1578:1588	the degradation	1574:1588	the degradation of pectin polymers	1574:1607	The latter was characterized by a reduction in signals for pectin epitopes in the berry cell walls, implying the degradation of pectin polymers.					
34192306	8	117	from	reduction	1499:1507	arg1	signals					1512:1518	signals	1512:1518	signals for pectin epitopes in the berry cell walls	1512:1562	The latter was characterized by a reduction in signals for pectin epitopes in the berry cell walls, implying the degradation of pectin polymers.					
34192306	0	118	theme	table	39:43	arg1	grapes					45:50	wine and table grapes	30:50	wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays	30:113	Tracking cell wall changes in wine and table grapes undergoing Botrytis cinerea infection using glycan microarrays.					
34192306	6	119	theme	scanning	1158:1165	arg1	SEM					1188:1190	SEM	1188:1190	SEM	1188:1190	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	6	119	theme	scanning	1158:1165	arg1	microscopy					1176:1185	scanning electron microscopy	1158:1185	scanning electron microscopy (SEM)	1158:1191	This was used in conjunction with scanning electron microscopy (SEM) and X-ray computed tomography (CT) to characterize infection progression.					
34192306	10	120	theme	arabinogalactan	1832:1846	arg1	content					1856:1862	the arabinogalactan protein content	1828:1862	the arabinogalactan protein content	1828:1862	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	4	121	theme	grape	639:643	arg1	composition					655:665	grape cell wall composition	639:665	grape cell wall composition	639:665	Limited studies have investigated changes in grape cell wall composition upon infection with B. cinerea, with much being inferred from studies on other fruit crops.					
34192306	10	122	theme	grape	1912:1916	arg1	cultivars					1918:1926	severely infected table grape cultivars	1888:1926	severely infected table grape cultivars	1888:1926	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	3	123	theme	many	484:487	arg1	interactions					504:515	many plant-pathogen interactions	484:515	many plant-pathogen interactions	484:515	The apoplast and cell wall interface is an important setting where many plant-pathogen interactions take place and where some defence-related messenger molecules are generated.					
34192306	1	124	theme	domesticated	224:235	arg1	vinifera					253:260	domesticated grapevine Vitis vinifera	224:260	domesticated grapevine Vitis vinifera cultivars	224:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
34192306	5	125	theme	table	1029:1033	arg1	Barlinka					1049:1056	Barlinka	1049:1056	Barlinka	1049:1056	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	5	125	theme	table	1029:1033	arg1	cultivars					1065:1073	wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars	979:1073	cultivars	1065:1073	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	5	125	theme	table	1029:1033	arg1	Dauphine					1036:1043	Dauphine	1036:1043	Dauphine	1036:1043	METHODS In this study, comprehensive microarray polymer profiling in combination with monosaccharide compositional analysis was applied for the first time to investigate cell wall compositional changes in the berries of wine (Sauvignon Blanc and Cabernet Sauvignon) and table (Dauphine and Barlinka) grape cultivars during Botrytis infection and tissue maceration.					
34192306	4	126	theme	wall	650:653	arg1	composition					655:665	grape cell wall composition	639:665	grape cell wall composition	639:665	Limited studies have investigated changes in grape cell wall composition upon infection with B. cinerea, with much being inferred from studies on other fruit crops.					
34192306	10	127	theme	epitope	1950:1956	arg1	deposition					1958:1967	extensin epitope deposition	1941:1967	extensin epitope deposition	1941:1967	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	12	128	theme	extensin	2228:2235	arg1	reorganization					2237:2250	extensin reorganization	2228:2250	extensin reorganization	2228:2250	Cell wall factors important in influencing resistance may include pectin methylesterification profiles, as well as extensin reorganization.					
34192306	10	129	theme	grape	1762:1766	arg1	types					1768:1772	both grape types	1757:1772	both grape types	1757:1772	In both grape types, hemicellulose layers were largely unaffected, as was the arabinogalactan protein content, whereas in moderate to severely infected table grape cultivars, evidence of extensin epitope deposition was present.					
34192306	1	130	theme	Vitis	247:251	arg1	vinifera					253:260	domesticated grapevine Vitis vinifera	224:260	domesticated grapevine Vitis vinifera cultivars	224:270	BACKGROUND AND AIMS The necrotrophic fungus Botrytis cinerea infects a broad range of fruit crops including domesticated grapevine Vitis vinifera cultivars.					
32463999	4	0	theme	3T3-L1	893:898	arg1	adipocytes					900:909	3T3-L1 adipocytes	893:909	3T3-L1 adipocytes	893:909	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	7	1	theme	GNJP	1490:1493	arg1	compositions					1420:1431	the nutritive compositions	1406:1431	the nutritive compositions	1406:1431	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	7	1	theme	GNJP	1490:1493	arg1	activities					1476:1485	significant glucose uptake stimulatory activities	1437:1485	significant glucose uptake stimulatory activities of GNJP	1437:1493	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	6	2	located	observed	1370:1377	arg1	study					1387:1391	this study	1382:1391	this study	1382:1391	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	2	located	observed	1370:1377	arg2	activities					1359:1368	the insulin-mimicking activities	1337:1368	the insulin-mimicking activities observed in this study	1337:1391	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	3	3	theme	carbohydrates	559:571	arg1	presence					547:554	a predominant presence	533:554	a predominant presence of carbohydrates, proteins, dietary fiber, and microelements	533:615	Nutritional analysis of G. neo-japonicum basidiocarps indicated a predominant presence of carbohydrates, proteins, dietary fiber, and microelements.					
32463999	6	4	theme	protein	1191:1197	arg1	AMP					1207:1209	AMP activated	1207:1219	AMP activated	1207:1219	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	4	theme	protein	1191:1197	arg1	kinase					1199:1204	protein kinase	1191:1204	protein kinase	1191:1204	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	4	5	theme	-β-D-glucan	670:680	arg1	GNJP					698:701	GNJP	698:701	GNJP	698:701	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	5	theme	-β-D-glucan	670:680	arg1	polysaccharide					682:695	its isolated (1,3)(1,6)-β-D-glucan polysaccharide	647:695	its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum	647:740	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	3	6	theme	fiber	592:596	arg1	presence					547:554	a predominant presence	533:554	a predominant presence of carbohydrates, proteins, dietary fiber, and microelements	533:615	Nutritional analysis of G. neo-japonicum basidiocarps indicated a predominant presence of carbohydrates, proteins, dietary fiber, and microelements.					
32463999	7	7	theme	uptake	1457:1462	arg1	activities					1476:1485	significant glucose uptake stimulatory activities	1437:1485	significant glucose uptake stimulatory activities of GNJP	1437:1493	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	6	8	theme	PRKAG2	1183:1188	arg1	expressions					1072:1082	the expressions	1068:1082	the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes	1068:1260	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	9	dep	PRKAG2	1183:1188	arg1	AMP					1207:1209	AMP activated	1207:1219	AMP activated	1207:1219	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	9	dep	PRKAG2	1183:1188	arg1	kinase					1199:1204	protein kinase	1191:1204	protein kinase	1191:1204	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	1	10	theme	polypore	199:206	arg1	mushroom					208:215	an annual polypore mushroom	189:215	an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes	189:308	Ganoderma neo-japonicum is an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes.					
32463999	1	10	theme	polypore	199:206	arg1	neo-japonicum					172:184	Ganoderma neo-japonicum	162:184	Ganoderma neo-japonicum	162:184	Ganoderma neo-japonicum is an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes.					
32463999	0	11	theme	Insulin-Like	123:134	arg1	Activity					136:143	Insulin-Like Activity	123:143	Insulin-Like Activity in 3T3-L1 Cells	123:159	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	2	12	theme	present	315:321	arg1	study					323:327	The present study	311:327	The present study	311:327	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	7	13	theme	related	1635:1641	arg1	complications					1643:1655	related complications	1635:1655	related complications	1635:1655	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	6	14	theme	glucose	1235:1241	arg1	Slc2a4					1227:1232	Slc2a4	1227:1232	Slc2a4 (glucose transporter)	1227:1254	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	14	theme	glucose	1235:1241	arg1	transporter					1243:1253	glucose transporter	1235:1253	glucose transporter	1235:1253	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	15	theme	Slc2a4	1227:1232	arg1	expressions					1072:1082	the expressions	1068:1082	the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes	1068:1260	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	4	16	theme	G.	725:726	arg1	neo-japonicum					728:740	G. neo-japonicum	725:740	G. neo-japonicum	725:740	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	7	17	theme	insulin	1611:1617	arg1	resistance					1619:1628	insulin resistance	1611:1628	insulin resistance	1611:1628	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	0	18	from	Potentiation	107:118	arg1	Cells					155:159	3T3-L1 Cells	148:159	3T3-L1 Cells	148:159	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	6	19	theme	3T3-L1	1293:1298	arg1	cells					1300:1304	3T3-L1 cells	1293:1304	3T3-L1 cells	1293:1304	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	5	20	theme	glucose	956:962	arg1	uptake					964:969	glucose uptake	956:969	glucose uptake	956:969	GNJP showed a dose dependent stimulation of glucose uptake and adiponectin secretion but attenuated lipid accumulation in 3T3-L1 adipocytes.					
32463999	7	21	theme	hyperglycemia	1596:1608	arg1	management					1582:1591	the management	1578:1591	the management of hyperglycemia, insulin resistance, and related complications	1578:1655	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	0	22	from	Composition	91:101	arg1	Cells					155:159	3T3-L1 Cells	148:159	3T3-L1 Cells	148:159	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	6	23	theme	activated	1211:1219	arg1	AMP					1207:1209	AMP activated	1207:1219	AMP activated	1207:1219	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	23	theme	activated	1211:1219	arg1	kinase					1199:1204	protein kinase	1191:1204	protein kinase	1191:1204	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	2	24	dep	in	380:381	arg1	vitro					383:387	vitro	383:387	vitro	383:387	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	4	25	from	expression	879:888	arg1	adipocytes					900:909	3T3-L1 adipocytes	893:909	3T3-L1 adipocytes	893:909	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	7	26	theme	functional	1558:1567	arg1	food					1569:1572	functional food	1558:1572	functional food	1558:1572	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	4	27	theme	independent	796:806	arg1	adipogenesis					808:819	insulin independent adipogenesis	788:819	insulin independent adipogenesis	788:819	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	27	theme	independent	796:806	arg1	uptake					830:835	glucose uptake	822:835	glucose uptake	822:835	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	27	theme	independent	796:806	arg1	secretion					850:858	adiponectin secretion	838:858	adiponectin secretion	838:858	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	2	28	theme	neo-japonicum	421:433	arg1	extracts					435:442	G. neo-japonicum extracts	418:442	G. neo-japonicum extracts	418:442	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	6	29	theme	kinase	1114:1119	arg1	Aktl					1100:1103	Aktl	1100:1103	Aktl (protein kinase B)	1100:1122	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	29	theme	kinase	1114:1119	arg1	B					1121:1121	protein kinase B	1106:1121	protein kinase B	1106:1121	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	3	30	theme	basidiocarps	510:521	arg1	analysis					481:488	Nutritional analysis	469:488	Nutritional analysis of G. neo-japonicum basidiocarps	469:521	Nutritional analysis of G. neo-japonicum basidiocarps indicated a predominant presence of carbohydrates, proteins, dietary fiber, and microelements.					
32463999	0	31	theme	Mushroom	14:21	arg1	Agaricomycetes					48:61	Agaricomycetes	48:61	Agaricomycetes	48:61	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	0	31	theme	Mushroom	14:21	arg1	neo-japonicum					33:45	The Medicinal Mushroom Ganoderma neo-japonicum	0:45	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.	0:160	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	7	32	theme	nutritive	1410:1418	arg1	compositions					1420:1431	the nutritive compositions	1406:1431	the nutritive compositions	1406:1431	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	5	33	theme	secretion	987:995	arg1	stimulation					941:951	a dose dependent stimulation	924:951	a dose dependent stimulation of glucose uptake and adiponectin secretion	924:995	GNJP showed a dose dependent stimulation of glucose uptake and adiponectin secretion but attenuated lipid accumulation in 3T3-L1 adipocytes.					
32463999	4	34	theme	Hot	618:620	arg1	AE					639:640	AE	639:640	AE	639:640	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	34	theme	Hot	618:620	arg1	extract					630:636	Hot aqueous extract	618:636	Hot aqueous extract (AE)	618:641	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	35	theme	adiponectin	838:848	arg1	adipogenesis					808:819	insulin independent adipogenesis	788:819	insulin independent adipogenesis	788:819	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	35	theme	adiponectin	838:848	arg1	secretion					850:858	adiponectin secretion	838:858	adiponectin secretion	838:858	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	3	36	theme	G.	493:494	arg1	basidiocarps					510:521	G. neo-japonicum basidiocarps	493:521	G. neo-japonicum basidiocarps	493:521	Nutritional analysis of G. neo-japonicum basidiocarps indicated a predominant presence of carbohydrates, proteins, dietary fiber, and microelements.					
32463999	0	37	dep	neo-japonicum	33:45	arg1	Potentiation					107:118	Potentiation	107:118	Potentiation	107:118	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	0	37	dep	neo-japonicum	33:45	arg1	Composition					91:101	Nutritional Composition	79:101	Nutritional Composition	79:101	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	6	38	theme	activated	1156:1164	arg1	PPARγ					1125:1129	PPARγ	1125:1129	PPARγ (peroxisome proliferator activated receptor gamma)	1125:1180	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	38	theme	activated	1156:1164	arg1	gamma					1175:1179	peroxisome proliferator activated receptor gamma	1132:1179	peroxisome proliferator activated receptor gamma	1132:1179	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	5	39	theme	3T3-L1	1034:1039	arg1	adipocytes					1041:1050	3T3-L1 adipocytes	1034:1050	3T3-L1 adipocytes	1034:1050	GNJP showed a dose dependent stimulation of glucose uptake and adiponectin secretion but attenuated lipid accumulation in 3T3-L1 adipocytes.					
32463999	6	40	theme	peroxisome	1132:1141	arg1	proliferator					1143:1154	peroxisome proliferator	1132:1154	peroxisome proliferator	1132:1154	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	2	41	theme	extracts	435:442	arg1	effects					407:413	in vitro antihyperglycemic effects	380:413	in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes	380:466	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	2	41	theme	extracts	435:442	arg1	composition					364:374	the nutritive composition	350:374	the nutritive composition	350:374	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	3	42	theme	proteins	574:581	arg1	presence					547:554	a predominant presence	533:554	a predominant presence of carbohydrates, proteins, dietary fiber, and microelements	533:615	Nutritional analysis of G. neo-japonicum basidiocarps indicated a predominant presence of carbohydrates, proteins, dietary fiber, and microelements.					
32463999	1	43	theme	indigenous	247:256	arg1	tribes					258:263	Malaysian indigenous tribes	237:263	Malaysian indigenous tribes	237:263	Ganoderma neo-japonicum is an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes.					
32463999	6	44	theme	PPARγ	1125:1129	arg1	expressions					1072:1082	the expressions	1068:1082	the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes	1068:1260	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	4	45	theme	isolated	651:658	arg1	GNJP					698:701	GNJP	698:701	GNJP	698:701	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	45	theme	isolated	651:658	arg1	polysaccharide					682:695	its isolated (1,3)(1,6)-β-D-glucan polysaccharide	647:695	its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum	647:740	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	2	46	theme	in	380:381	arg1	effects					407:413	in vitro antihyperglycemic effects	380:413	in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes	380:466	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	5	47	from	accumulation	1018:1029	arg1	adipocytes					1041:1050	3T3-L1 adipocytes	1034:1050	3T3-L1 adipocytes	1034:1050	GNJP showed a dose dependent stimulation of glucose uptake and adiponectin secretion but attenuated lipid accumulation in 3T3-L1 adipocytes.					
32463999	6	48	theme	glucose	1275:1281	arg1	uptake					1283:1288	glucose uptake	1275:1288	glucose uptake	1275:1288	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	1	49	theme	various	274:280	arg1	diabetes					301:308	diabetes	301:308	diabetes	301:308	Ganoderma neo-japonicum is an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes.					
32463999	1	49	theme	various	274:280	arg1	ailments					282:289	various ailments	274:289	various ailments including diabetes	274:308	Ganoderma neo-japonicum is an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes.					
32463999	7	50	theme	stimulatory	1464:1474	arg1	activities					1476:1485	significant glucose uptake stimulatory activities	1437:1485	significant glucose uptake stimulatory activities of GNJP	1437:1493	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	2	51	from	composition	364:374	arg1	preadipocytes					454:466	3T3-L1 preadipocytes	447:466	3T3-L1 preadipocytes	447:466	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	7	52	theme	significant	1437:1447	arg1	uptake					1457:1462	significant glucose uptake	1437:1462	significant glucose uptake stimulatory activities of GNJP	1437:1493	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	0	53	from	Cells	155:159	arg1	Potentiation					107:118	Potentiation	107:118	Potentiation	107:118	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	0	53	from	Cells	155:159	arg1	Composition					91:101	Nutritional Composition	79:101	Nutritional Composition	79:101	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	3	54	theme	dietary	584:590	arg1	fiber					592:596	dietary fiber	584:596	dietary fiber	584:596	Nutritional analysis of G. neo-japonicum basidiocarps indicated a predominant presence of carbohydrates, proteins, dietary fiber, and microelements.					
32463999	7	55	theme	glucose	1449:1455	arg1	uptake					1457:1462	significant glucose uptake	1437:1462	significant glucose uptake stimulatory activities of GNJP	1437:1493	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	1	56	theme	annual	192:197	arg1	mushroom					208:215	an annual polypore mushroom	189:215	an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes	189:308	Ganoderma neo-japonicum is an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes.					
32463999	1	56	theme	annual	192:197	arg1	neo-japonicum					172:184	Ganoderma neo-japonicum	162:184	Ganoderma neo-japonicum	162:184	Ganoderma neo-japonicum is an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes.					
32463999	7	57	theme	complications	1643:1655	arg1	management					1582:1591	the management	1578:1591	the management of hyperglycemia, insulin resistance, and related complications	1578:1655	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	4	58	theme	gene	874:877	arg1	expression					879:888	gene expression	874:888	gene expression in 3T3-L1 adipocytes	874:909	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	0	59	theme	Activity	136:143	arg1	Potentiation					107:118	Potentiation	107:118	Potentiation	107:118	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	0	59	theme	Activity	136:143	arg1	Composition					91:101	Nutritional Composition	79:101	Nutritional Composition	79:101	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	3	60	theme	microelements	603:615	arg1	presence					547:554	a predominant presence	533:554	a predominant presence of carbohydrates, proteins, dietary fiber, and microelements	533:615	Nutritional analysis of G. neo-japonicum basidiocarps indicated a predominant presence of carbohydrates, proteins, dietary fiber, and microelements.					
32463999	2	61	theme	antihyperglycemic	389:405	arg1	effects					407:413	in vitro antihyperglycemic effects	380:413	in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes	380:466	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	0	62	theme	3T3-L1	148:153	arg1	Cells					155:159	3T3-L1 Cells	148:159	3T3-L1 Cells	148:159	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	7	63	contain	have	1517:1520	arg1	it					1510:1511	it	1510:1511	it	1510:1511	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	7	63	contain	have	1517:1520	arg2	use					1532:1534	potential use	1522:1534	potential use	1522:1534	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	7	64	theme	resistance	1619:1628	arg1	management					1582:1591	the management	1578:1591	the management of hyperglycemia, insulin resistance, and related complications	1578:1655	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	0	65	from	Malaysia	69:76	arg1	Agaricomycetes					48:61	Agaricomycetes	48:61	Agaricomycetes	48:61	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	0	65	from	Malaysia	69:76	arg1	neo-japonicum					33:45	The Medicinal Mushroom Ganoderma neo-japonicum	0:45	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.	0:160	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	5	66	theme	dependent	931:939	arg1	stimulation					941:951	a dose dependent stimulation	924:951	a dose dependent stimulation of glucose uptake and adiponectin secretion	924:995	GNJP showed a dose dependent stimulation of glucose uptake and adiponectin secretion but attenuated lipid accumulation in 3T3-L1 adipocytes.					
32463999	4	67	dep	isolated	651:658	arg1	1,3					661:663	1,3	661:663	1,3	661:663	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	67	dep	isolated	651:658	arg1	1,6					666:668	1,6	666:668	1,6	666:668	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	6	68	dep	adiponectin	1087:1097	arg1	genes					1256:1260	genes	1256:1260	genes	1256:1260	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	4	69	theme	neo-japonicum	728:740	arg1	basidiocarps					709:720	basidiocarps	709:720	basidiocarps of G. neo-japonicum	709:740	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	0	70	theme	Nutritional	79:89	arg1	Composition					91:101	Nutritional Composition	79:101	Nutritional Composition	79:101	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	1	71	theme	Ganoderma	162:170	arg1	mushroom					208:215	an annual polypore mushroom	189:215	an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes	189:308	Ganoderma neo-japonicum is an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes.					
32463999	1	71	theme	Ganoderma	162:170	arg1	neo-japonicum					172:184	Ganoderma neo-japonicum	162:184	Ganoderma neo-japonicum	162:184	Ganoderma neo-japonicum is an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes.					
32463999	7	72	theme	food	1569:1572	arg1	formulation					1543:1553	the formulation	1539:1553	the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications	1539:1655	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	6	73	theme	insulin-mimicking	1341:1357	arg1	activities					1359:1368	the insulin-mimicking activities	1337:1368	the insulin-mimicking activities observed in this study	1337:1391	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	5	74	theme	lipid	1012:1016	arg1	accumulation					1018:1029	lipid accumulation	1012:1029	lipid accumulation in 3T3-L1 adipocytes	1012:1050	GNJP showed a dose dependent stimulation of glucose uptake and adiponectin secretion but attenuated lipid accumulation in 3T3-L1 adipocytes.					
32463999	4	75	theme	insulin	788:794	arg1	adipogenesis					808:819	insulin independent adipogenesis	788:819	insulin independent adipogenesis	788:819	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	75	theme	insulin	788:794	arg1	uptake					830:835	glucose uptake	822:835	glucose uptake	822:835	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	75	theme	insulin	788:794	arg1	secretion					850:858	adiponectin secretion	838:858	adiponectin secretion	838:858	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	2	76	theme	G.	418:419	arg1	extracts					435:442	G. neo-japonicum extracts	418:442	G. neo-japonicum extracts	418:442	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	0	77	theme	Medicinal	4:12	arg1	Agaricomycetes					48:61	Agaricomycetes	48:61	Agaricomycetes	48:61	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	0	77	theme	Medicinal	4:12	arg1	neo-japonicum					33:45	The Medicinal Mushroom Ganoderma neo-japonicum	0:45	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.	0:160	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	5	78	theme	uptake	964:969	arg1	stimulation					941:951	a dose dependent stimulation	924:951	a dose dependent stimulation of glucose uptake and adiponectin secretion	924:995	GNJP showed a dose dependent stimulation of glucose uptake and adiponectin secretion but attenuated lipid accumulation in 3T3-L1 adipocytes.					
32463999	6	79	theme	protein	1106:1112	arg1	Aktl					1100:1103	Aktl	1100:1103	Aktl (protein kinase B)	1100:1122	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	79	theme	protein	1106:1112	arg1	B					1121:1121	protein kinase B	1106:1121	protein kinase B	1106:1121	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	3	80	theme	Nutritional	469:479	arg1	analysis					481:488	Nutritional analysis	469:488	Nutritional analysis of G. neo-japonicum basidiocarps	469:521	Nutritional analysis of G. neo-japonicum basidiocarps indicated a predominant presence of carbohydrates, proteins, dietary fiber, and microelements.					
32463999	0	81	theme	Ganoderma	23:31	arg1	Agaricomycetes					48:61	Agaricomycetes	48:61	Agaricomycetes	48:61	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	0	81	theme	Ganoderma	23:31	arg1	neo-japonicum					33:45	The Medicinal Mushroom Ganoderma neo-japonicum	0:45	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.	0:160	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	5	82	theme	adiponectin	975:985	arg1	secretion					987:995	adiponectin secretion	975:995	adiponectin secretion	975:995	GNJP showed a dose dependent stimulation of glucose uptake and adiponectin secretion but attenuated lipid accumulation in 3T3-L1 adipocytes.					
32463999	6	83	theme	Aktl	1100:1103	arg1	expressions					1072:1082	the expressions	1068:1082	the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes	1068:1260	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	4	84	theme	aqueous	622:628	arg1	AE					639:640	AE	639:640	AE	639:640	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	84	theme	aqueous	622:628	arg1	extract					630:636	Hot aqueous extract	618:636	Hot aqueous extract (AE)	618:641	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	2	85	theme	3T3-L1	447:452	arg1	preadipocytes					454:466	3T3-L1 preadipocytes	447:466	3T3-L1 preadipocytes	447:466	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	6	86	theme	adiponectin	1087:1097	arg1	expressions					1072:1082	the expressions	1068:1082	the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes	1068:1260	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	3	87	theme	neo-japonicum	496:508	arg1	basidiocarps					510:521	G. neo-japonicum basidiocarps	493:521	G. neo-japonicum basidiocarps	493:521	Nutritional analysis of G. neo-japonicum basidiocarps indicated a predominant presence of carbohydrates, proteins, dietary fiber, and microelements.					
32463999	2	88	from	effects	407:413	arg1	preadipocytes					454:466	3T3-L1 preadipocytes	447:466	3T3-L1 preadipocytes	447:466	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	6	89	theme	receptor	1166:1173	arg1	PPARγ					1125:1129	PPARγ	1125:1129	PPARγ (peroxisome proliferator activated receptor gamma)	1125:1180	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	6	89	theme	receptor	1166:1173	arg1	gamma					1175:1179	peroxisome proliferator activated receptor gamma	1132:1179	peroxisome proliferator activated receptor gamma	1132:1179	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	2	90	theme	nutritive	354:362	arg1	composition					364:374	the nutritive composition	350:374	the nutritive composition	350:374	The present study aimed to investigate the nutritive composition and in vitro antihyperglycemic effects of G. neo-japonicum extracts on 3T3-L1 preadipocytes.					
32463999	4	91	theme	glucose	822:828	arg1	adipogenesis					808:819	insulin independent adipogenesis	788:819	insulin independent adipogenesis	788:819	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	91	theme	glucose	822:828	arg1	uptake					830:835	glucose uptake	822:835	glucose uptake	822:835	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	7	92	theme	potential	1522:1530	arg1	use					1532:1534	potential use	1522:1534	potential use	1522:1534	In summary, the nutritive compositions and significant glucose uptake stimulatory activities of GNJP indicated that it may have potential use in the formulation of functional food for the management of hyperglycemia, insulin resistance, and related complications.					
32463999	3	93	theme	predominant	535:545	arg1	presence					547:554	a predominant presence	533:554	a predominant presence of carbohydrates, proteins, dietary fiber, and microelements	533:615	Nutritional analysis of G. neo-japonicum basidiocarps indicated a predominant presence of carbohydrates, proteins, dietary fiber, and microelements.					
32463999	1	94	theme	Malaysian	237:245	arg1	tribes					258:263	Malaysian indigenous tribes	237:263	Malaysian indigenous tribes	237:263	Ganoderma neo-japonicum is an annual polypore mushroom that is consumed by Malaysian indigenous tribes to treat various ailments including diabetes.					
32463999	0	95	from	Activity	136:143	arg1	Cells					155:159	3T3-L1 Cells	148:159	3T3-L1 Cells	148:159	The Medicinal Mushroom Ganoderma neo-japonicum (Agaricomycetes) from Malaysia: Nutritional Composition and Potentiation of Insulin-Like Activity in 3T3-L1 Cells.					
32463999	6	96	dep	activated	1156:1164	arg1	proliferator					1143:1154	peroxisome proliferator	1132:1154	peroxisome proliferator	1132:1154	It upregulated the expressions of adiponectin, Aktl (protein kinase B), PPARγ (peroxisome proliferator activated receptor gamma), PRKAG2 (protein kinase, AMP activated), and Slc2a4 (glucose transporter) genes to stimulate glucose uptake in 3T3-L1 cells, which may have contributed to the insulin-mimicking activities observed in this study.					
32463999	4	97	from	basidiocarps	709:720	arg1	AE					639:640	AE	639:640	AE	639:640	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	97	from	basidiocarps	709:720	arg1	extract					630:636	Hot aqueous extract	618:636	Hot aqueous extract (AE)	618:641	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	97	from	basidiocarps	709:720	arg1	polysaccharide					682:695	its isolated (1,3)(1,6)-β-D-glucan polysaccharide	647:695	its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum	647:740	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32463999	4	97	from	basidiocarps	709:720	arg1	GNJP					698:701	GNJP	698:701	GNJP	698:701	Hot aqueous extract (AE) and its isolated (1,3)(1,6)-β-D-glucan polysaccharide (GNJP) from basidiocarps of G. neo-japonicum were evaluated for their ability to stimulate insulin independent adipogenesis, glucose uptake, adiponectin secretion, and regulate gene expression in 3T3-L1 adipocytes.					
32335047	0	0	theme	α-amylase	62:70	arg1	activity					72:79	α-amylase activity	62:79	α-amylase activity	62:79	A neutral polysaccharide from green tea: Structure, effect on α-amylase activity and hydrolysis property.					
32335047	4	1	theme	nuclear	457:463	arg1	NMR					485:487	NMR	485:487	NMR	485:487	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	4	1	theme	nuclear	457:463	arg1	resonance					474:482	nuclear magnetic resonance	457:482	nuclear magnetic resonance (NMR) spectroscopy	457:501	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	5	2	theme	circular	760:767	arg1	spectroscopy					779:790	circular dichroism spectroscopy	760:790	circular dichroism spectroscopy	760:790	TPSN exhibited inhibitory activity towards α-amylase through the noncompetitive inhibition mechanism, but the tertiary structure of α-amylase related to enzymatic activity, analyzed using circular dichroism spectroscopy, was not affected by TPSN.					
32335047	7	3	theme	docking	901:907	arg1	analysis					909:916	Molecular docking analysis	891:916	Molecular docking analysis	891:916	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	5	4	theme	dichroism	769:777	arg1	spectroscopy					779:790	circular dichroism spectroscopy	760:790	circular dichroism spectroscopy	760:790	TPSN exhibited inhibitory activity towards α-amylase through the noncompetitive inhibition mechanism, but the tertiary structure of α-amylase related to enzymatic activity, analyzed using circular dichroism spectroscopy, was not affected by TPSN.					
32335047	5	5	theme	noncompetitive	637:650	arg1	mechanism					663:671	the noncompetitive inhibition mechanism	633:671	the noncompetitive inhibition mechanism	633:671	TPSN exhibited inhibitory activity towards α-amylase through the noncompetitive inhibition mechanism, but the tertiary structure of α-amylase related to enzymatic activity, analyzed using circular dichroism spectroscopy, was not affected by TPSN.					
32335047	4	6	theme	resonance	474:482	arg1	spectroscopy					490:501	nuclear magnetic resonance (NMR) spectroscopy	457:501	nuclear magnetic resonance (NMR) spectroscopy	457:501	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	5	7	theme	inhibition	652:661	arg1	mechanism					663:671	the noncompetitive inhibition mechanism	633:671	the noncompetitive inhibition mechanism	633:671	TPSN exhibited inhibitory activity towards α-amylase through the noncompetitive inhibition mechanism, but the tertiary structure of α-amylase related to enzymatic activity, analyzed using circular dichroism spectroscopy, was not affected by TPSN.					
32335047	2	8	from	ratio	291:295	arg1	residues					271:278	d-glucose, l-arabinose and d-galactose residues	232:278	d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9	232:313	Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9.					
32335047	2	9	theme	d-galactose	259:269	arg1	residues					271:278	d-glucose, l-arabinose and d-galactose residues	232:278	d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9	232:313	Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9.					
32335047	5	10	theme	enzymatic	725:733	arg1	activity					735:742	enzymatic activity	725:742	enzymatic activity	725:742	TPSN exhibited inhibitory activity towards α-amylase through the noncompetitive inhibition mechanism, but the tertiary structure of α-amylase related to enzymatic activity, analyzed using circular dichroism spectroscopy, was not affected by TPSN.					
32335047	4	11	theme	similar	552:558	arg1	structures					560:569	similar structures	552:569	similar structures	552:569	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	5	12	theme	inhibitory	587:596	arg1	activity					598:605	inhibitory activity	587:605	inhibitory activity towards α-amylase	587:623	TPSN exhibited inhibitory activity towards α-amylase through the noncompetitive inhibition mechanism, but the tertiary structure of α-amylase related to enzymatic activity, analyzed using circular dichroism spectroscopy, was not affected by TPSN.					
32335047	2	13	theme	l-arabinose	243:253	arg1	residues					271:278	d-glucose, l-arabinose and d-galactose residues	232:278	d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9	232:313	Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9.					
32335047	0	14	theme	hydrolysis	85:94	arg1	property					96:103	hydrolysis property	85:103	hydrolysis property	85:103	A neutral polysaccharide from green tea: Structure, effect on α-amylase activity and hydrolysis property.					
32335047	0	15	from	tea	36:38	arg1	polysaccharide					10:23	A neutral polysaccharide	0:23	A neutral polysaccharide from green tea	0:38	A neutral polysaccharide from green tea: Structure, effect on α-amylase activity and hydrolysis property.					
32335047	0	15	from	tea	36:38	arg1	effect					52:57	effect	52:57	effect on α-amylase activity and hydrolysis property	52:103	A neutral polysaccharide from green tea: Structure, effect on α-amylase activity and hydrolysis property.					
32335047	0	16	theme	neutral	2:8	arg1	polysaccharide					10:23	A neutral polysaccharide	0:23	A neutral polysaccharide from green tea	0:38	A neutral polysaccharide from green tea: Structure, effect on α-amylase activity and hydrolysis property.					
32335047	0	16	theme	neutral	2:8	arg1	effect					52:57	effect	52:57	effect on α-amylase activity and hydrolysis property	52:103	A neutral polysaccharide from green tea: Structure, effect on α-amylase activity and hydrolysis property.					
32335047	7	17	theme	α-amylase	1087:1095	arg1	α-amylase					1087:1095	α-amylase	1087:1095	α-amylase	1087:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	7	17	theme	α-amylase	1087:1095	arg1	residues					1075:1082	different amino acid residues	1054:1082	different amino acid residues of α-amylase	1054:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	7	18	theme	TPSN	957:960	arg1	behaviors					944:952	the various behaviors	932:952	the various behaviors of TPSN to α-amylase	932:973	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	2	19	theme	molar	285:289	arg1	ratio					291:295	a molar ratio	283:295	a molar ratio of 90.0: 9.1: 0.9	283:313	Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9.					
32335047	7	20	theme	amino	1064:1068	arg1	α-amylase					1087:1095	α-amylase	1087:1095	α-amylase	1087:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	7	20	theme	amino	1064:1068	arg1	residues					1075:1082	different amino acid residues	1054:1082	different amino acid residues of α-amylase	1054:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	7	21	theme	various	936:942	arg1	behaviors					944:952	the various behaviors	932:952	the various behaviors of TPSN to α-amylase	932:973	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	3	22	theme	scattering	422:431	arg1	analysis					433:440	static light scattering analysis	409:440	static light scattering analysis	409:440	The weight-averaged molecular weight of TPSN was determined as about 2.0 × 105 g mol-1 using static light scattering analysis.					
32335047	3	23	theme	weight-averaged	320:334	arg1	2.0 × 105 g mol-1					385:401	about 2.0 × 105 g mol-1	379:401	about 2.0 × 105 g mol-1 using static light scattering analysis	379:440	The weight-averaged molecular weight of TPSN was determined as about 2.0 × 105 g mol-1 using static light scattering analysis.					
32335047	3	23	theme	weight-averaged	320:334	arg1	weight					346:351	The weight-averaged molecular weight	316:351	The weight-averaged molecular weight of TPSN	316:359	The weight-averaged molecular weight of TPSN was determined as about 2.0 × 105 g mol-1 using static light scattering analysis.					
32335047	4	24	theme	water-soluble	527:539	arg1	starch					541:546	water-soluble starch	527:546	water-soluble starch	527:546	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	1	25	attach	isolated	146:153	arg2	TPSN					136:139	TPSN	136:139	TPSN	136:139	A neutral tea polysaccharide (TPSN) was isolated from green tea.					
32335047	1	25	attach	isolated	146:153	arg2	polysaccharide					120:133	A neutral tea polysaccharide	106:133	A neutral tea polysaccharide (TPSN)	106:140	A neutral tea polysaccharide (TPSN) was isolated from green tea.					
32335047	1	25	attach	isolated	146:153	arg1	tea					166:168	green tea	160:168	green tea	160:168	A neutral tea polysaccharide (TPSN) was isolated from green tea.					
32335047	2	26	dep	90.0	300:303	arg1	0.9					311:313	0.9	311:313	0.9	311:313	Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9.					
32335047	2	26	dep	90.0	300:303	arg1	9.1					306:308	9.1	306:308	9.1	306:308	Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9.					
32335047	5	27	theme	α-amylase	704:712	arg1	structure					691:699	the tertiary structure	678:699	the tertiary structure	678:699	TPSN exhibited inhibitory activity towards α-amylase through the noncompetitive inhibition mechanism, but the tertiary structure of α-amylase related to enzymatic activity, analyzed using circular dichroism spectroscopy, was not affected by TPSN.					
32335047	3	28	theme	static	409:414	arg1	analysis					433:440	static light scattering analysis	409:440	static light scattering analysis	409:440	The weight-averaged molecular weight of TPSN was determined as about 2.0 × 105 g mol-1 using static light scattering analysis.					
32335047	5	29	theme	related	714:720	arg1	structure					691:699	the tertiary structure	678:699	the tertiary structure	678:699	TPSN exhibited inhibitory activity towards α-amylase through the noncompetitive inhibition mechanism, but the tertiary structure of α-amylase related to enzymatic activity, analyzed using circular dichroism spectroscopy, was not affected by TPSN.					
32335047	0	30	from	effect	52:57	arg1	activity					72:79	α-amylase activity	62:79	α-amylase activity	62:79	A neutral polysaccharide from green tea: Structure, effect on α-amylase activity and hydrolysis property.					
32335047	0	30	from	effect	52:57	arg1	property					96:103	hydrolysis property	85:103	hydrolysis property	85:103	A neutral polysaccharide from green tea: Structure, effect on α-amylase activity and hydrolysis property.					
32335047	3	31	theme	light	416:420	arg1	analysis					433:440	static light scattering analysis	409:440	static light scattering analysis	409:440	The weight-averaged molecular weight of TPSN was determined as about 2.0 × 105 g mol-1 using static light scattering analysis.					
32335047	6	32	theme	hydrolysis	845:854	arg1	properties					856:865	hydrolysis properties	845:865	hydrolysis properties catalyzed by α-amylase	845:888	Meanwhile, TPSN exhibited hydrolysis properties catalyzed by α-amylase.					
32335047	7	33	theme	acid	1070:1073	arg1	α-amylase					1087:1095	α-amylase	1087:1095	α-amylase	1087:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	7	33	theme	acid	1070:1073	arg1	residues					1075:1082	different amino acid residues	1054:1082	different amino acid residues of α-amylase	1054:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	3	34	theme	TPSN	356:359	arg1	2.0 × 105 g mol-1					385:401	about 2.0 × 105 g mol-1	379:401	about 2.0 × 105 g mol-1 using static light scattering analysis	379:440	The weight-averaged molecular weight of TPSN was determined as about 2.0 × 105 g mol-1 using static light scattering analysis.					
32335047	3	34	theme	TPSN	356:359	arg1	weight					346:351	The weight-averaged molecular weight	316:351	The weight-averaged molecular weight of TPSN	316:359	The weight-averaged molecular weight of TPSN was determined as about 2.0 × 105 g mol-1 using static light scattering analysis.					
32335047	7	35	theme	different	1054:1062	arg1	α-amylase					1087:1095	α-amylase	1087:1095	α-amylase	1087:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	7	35	theme	different	1054:1062	arg1	residues					1075:1082	different amino acid residues	1054:1082	different amino acid residues of α-amylase	1054:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	2	36	theme	chromatography	175:188	arg1	analysis					190:197	Gas chromatography analysis	171:197	Gas chromatography analysis	171:197	Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9.					
32335047	4	37	contain	had	548:550	arg1	TPSN					518:521	TPSN	518:521	TPSN	518:521	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	4	37	contain	had	548:550	arg1	starch					541:546	water-soluble starch	527:546	water-soluble starch	527:546	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	4	37	contain	had	548:550	arg2	structures					560:569	similar structures	552:569	similar structures	552:569	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	7	38	theme	different	1007:1015	arg1	segments					1023:1030	that the different chain segments	998:1030	that the different chain segments of TPSN combined with different amino acid residues of α-amylase	998:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	2	39	theme	90.0	300:303	arg1	ratio					291:295	a molar ratio	283:295	a molar ratio of 90.0: 9.1: 0.9	283:313	Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9.					
32335047	4	40	theme	spectroscopy	490:501	arg1	result					447:452	The result	443:452	The result of nuclear magnetic resonance (NMR) spectroscopy	443:501	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	7	41	theme	TPSN	1035:1038	arg1	segments					1023:1030	that the different chain segments	998:1030	that the different chain segments of TPSN combined with different amino acid residues of α-amylase	998:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	2	42	theme	Gas	171:173	arg1	analysis					190:197	Gas chromatography analysis	171:197	Gas chromatography analysis	171:197	Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9.					
32335047	2	43	theme	d-glucose	232:240	arg1	residues					271:278	d-glucose, l-arabinose and d-galactose residues	232:278	d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9	232:313	Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0: 9.1: 0.9.					
32335047	7	44	theme	chain	1017:1021	arg1	segments					1023:1030	that the different chain segments	998:1030	that the different chain segments of TPSN combined with different amino acid residues of α-amylase	998:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	1	45	theme	neutral	108:114	arg1	TPSN					136:139	TPSN	136:139	TPSN	136:139	A neutral tea polysaccharide (TPSN) was isolated from green tea.					
32335047	1	45	theme	neutral	108:114	arg1	polysaccharide					120:133	A neutral tea polysaccharide	106:133	A neutral tea polysaccharide (TPSN)	106:140	A neutral tea polysaccharide (TPSN) was isolated from green tea.					
32335047	5	46	theme	tertiary	682:689	arg1	structure					691:699	the tertiary structure	678:699	the tertiary structure	678:699	TPSN exhibited inhibitory activity towards α-amylase through the noncompetitive inhibition mechanism, but the tertiary structure of α-amylase related to enzymatic activity, analyzed using circular dichroism spectroscopy, was not affected by TPSN.					
32335047	0	47	dep	polysaccharide	10:23	arg1	Structure					41:49	Structure	41:49	Structure	41:49	A neutral polysaccharide from green tea: Structure, effect on α-amylase activity and hydrolysis property.					
32335047	7	48	theme	that	998:1001	arg1	segments					1023:1030	that the different chain segments	998:1030	that the different chain segments of TPSN combined with different amino acid residues of α-amylase	998:1095	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
32335047	1	49	theme	tea	116:118	arg1	TPSN					136:139	TPSN	136:139	TPSN	136:139	A neutral tea polysaccharide (TPSN) was isolated from green tea.					
32335047	1	49	theme	tea	116:118	arg1	polysaccharide					120:133	A neutral tea polysaccharide	106:133	A neutral tea polysaccharide (TPSN)	106:140	A neutral tea polysaccharide (TPSN) was isolated from green tea.					
32335047	4	50	theme	magnetic	465:472	arg1	NMR					485:487	NMR	485:487	NMR	485:487	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	4	50	theme	magnetic	465:472	arg1	resonance					474:482	nuclear magnetic resonance	457:482	nuclear magnetic resonance (NMR) spectroscopy	457:501	The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures.					
32335047	3	51	theme	molecular	336:344	arg1	2.0 × 105 g mol-1					385:401	about 2.0 × 105 g mol-1	379:401	about 2.0 × 105 g mol-1 using static light scattering analysis	379:440	The weight-averaged molecular weight of TPSN was determined as about 2.0 × 105 g mol-1 using static light scattering analysis.					
32335047	3	51	theme	molecular	336:344	arg1	weight					346:351	The weight-averaged molecular weight	316:351	The weight-averaged molecular weight of TPSN	316:359	The weight-averaged molecular weight of TPSN was determined as about 2.0 × 105 g mol-1 using static light scattering analysis.					
32335047	7	52	theme	Molecular	891:899	arg1	analysis					909:916	Molecular docking analysis	891:916	Molecular docking analysis	891:916	Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase.					
34841862	2	0	from	roles	420:424	arg1	processes					443:451	these processes	437:451	these processes	437:451	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	3	1	theme	AD	673:674	arg1	patients					676:683	AD patients	673:683	AD patients	673:683	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	3	2	theme	patients	676:683	arg1	samples					662:668	the cerebrospinal fluid samples	638:668	the cerebrospinal fluid samples of AD patients	638:683	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	2	3	theme	Aβ	269:270	arg1	peptides					272:279	Aβ peptides	269:279	Aβ peptides	269:279	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	2	4	theme	posttranslational	294:310	arg1	PTMs					327:330	PTMs	327:330	PTMs	327:330	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	2	4	theme	posttranslational	294:310	arg1	modifications					312:324	diverse posttranslational modifications	286:324	diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils	286:383	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	5	5	theme	new	909:911	arg1	stable					955:960	stable	955:960	stable	955:960	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	5	5	theme	new	909:911	arg1	structure					932:940	a new fibril polymorphic structure	907:940	a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme)	907:1037	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	6	6	theme	AD-related	1285:1294	arg1	neurotoxicity					1296:1308	AD-related neurotoxicity	1285:1308	AD-related neurotoxicity	1285:1308	Thus, beyond showing how particular O-glycosylation modifications affect Aβ42 aggregation at the molecular level, our study provides powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity.					
34841862	6	7	theme	O-glycosylation	1076:1090	arg1	modifications					1092:1104	particular O-glycosylation modifications	1065:1104	particular O-glycosylation modifications	1065:1104	Thus, beyond showing how particular O-glycosylation modifications affect Aβ42 aggregation at the molecular level, our study provides powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity.					
34841862	4	8	theme	Aβ42	776:779	arg1	degradation					761:771	degradation	761:771	degradation	761:771	We discovered that O-glycans significantly affect both the aggregation and degradation of Aβ42.					
34841862	4	8	theme	Aβ42	776:779	arg1	aggregation					745:755	aggregation	745:755	aggregation	745:755	We discovered that O-glycans significantly affect both the aggregation and degradation of Aβ42.					
34841862	1	9	theme	disrupted	156:164	arg1	balance					166:172	a disrupted balance	154:172	a disrupted balance between biosynthesis and clearance	154:207	Brain accumulation of amyloid-β (Aβ) peptides (resulting from a disrupted balance between biosynthesis and clearance) occurs during the progression of Alzheimer's disease (AD).					
34841862	3	10	theme	modified	526:533	arg1	isoforms					535:542	three homogeneously modified isoforms	506:542	three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation	506:594	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	3	10	theme	modified	526:533	arg1	PTM					608:610	an unusual PTM	597:610	an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients	597:683	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	5	11	theme	Galβ1-3GalNAc	857:869	arg1	modification					871:882	a Galβ1-3GalNAc modification	855:882	a Galβ1-3GalNAc modification	855:882	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	1	12	theme	Brain	92:96	arg1	accumulation					98:109	Brain accumulation	92:109	Brain accumulation of amyloid-β (Aβ) peptides (resulting from a disrupted balance between biosynthesis and clearance)	92:208	Brain accumulation of amyloid-β (Aβ) peptides (resulting from a disrupted balance between biosynthesis and clearance) occurs during the progression of Alzheimer's disease (AD).					
34841862	2	13	theme	PTMs	429:432	arg1	roles					420:424	the mechanistic roles	404:424	the mechanistic roles of PTMs in these processes	404:451	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	6	14	theme	fibril	1262:1267	arg1	aggregation					1269:1279	Aβ42 fibril aggregation	1257:1279	Aβ42 fibril aggregation	1257:1279	Thus, beyond showing how particular O-glycosylation modifications affect Aβ42 aggregation at the molecular level, our study provides powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity.					
34841862	2	15	theme	diverse	286:292	arg1	PTMs					327:330	PTMs	327:330	PTMs	327:330	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	2	15	theme	diverse	286:292	arg1	modifications					312:324	diverse posttranslational modifications	286:324	diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils	286:383	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	5	16	theme	biochemical	815:825	arg1	assays					827:832	various biochemical assays	807:832	various biochemical assays	807:832	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	5	17	theme	various	807:813	arg1	assays					827:832	various biochemical assays	807:832	various biochemical assays	807:832	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	3	18	theme	Aβ	547:548	arg1	peptides					557:564	Aβ (1-42) peptides	547:564	Aβ (1-42) peptides bearing Tyr10 O-glycosylation	547:594	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	6	19	theme	Aβ42	1257:1260	arg1	aggregation					1269:1279	Aβ42 fibril aggregation	1257:1279	Aβ42 fibril aggregation	1257:1279	Thus, beyond showing how particular O-glycosylation modifications affect Aβ42 aggregation at the molecular level, our study provides powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity.					
34841862	2	20	theme	mechanistic	408:418	arg1	roles					420:424	the mechanistic roles	404:424	the mechanistic roles of PTMs in these processes	404:451	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	6	21	theme	experimental	1182:1193	arg1	tools					1195:1199	powerful experimental tools	1173:1199	powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity	1173:1308	Thus, beyond showing how particular O-glycosylation modifications affect Aβ42 aggregation at the molecular level, our study provides powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity.					
34841862	0	22	theme	New	42:44	arg1	Polymorphs					53:62	New Fibril Polymorphs	42:62	New Fibril Polymorphs Vulnerable for Degradation	42:89	O-Glycosylation Induces Amyloid-β To Form New Fibril Polymorphs Vulnerable for Degradation.					
34841862	6	23	theme	molecular	1137:1145	arg1	level					1147:1151	the molecular level	1133:1151	the molecular level	1133:1151	Thus, beyond showing how particular O-glycosylation modifications affect Aβ42 aggregation at the molecular level, our study provides powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity.					
34841862	3	24	theme	Tyr10	574:578	arg1	O-glycosylation					580:594	Tyr10 O-glycosylation	574:594	Tyr10 O-glycosylation	574:594	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	5	25	dep	enzyme	1031:1036	arg1	e.g.					1007:1010	e.g.	1007:1010	e.g.	1007:1010	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	5	26	theme	insulin-degrading	1013:1029	arg1	enzyme					1031:1036	insulin-degrading enzyme	1013:1036	insulin-degrading enzyme	1013:1036	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	2	27	contain	have	281:284	arg2	modifications					312:324	diverse posttranslational modifications	286:324	diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils	286:383	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	2	27	contain	have	281:284	arg1	peptides					272:279	Aβ peptides	269:279	Aβ peptides	269:279	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	2	27	contain	have	281:284	arg2	PTMs					327:330	PTMs	327:330	PTMs	327:330	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	3	28	theme	peptides	557:564	arg1	isoforms					535:542	three homogeneously modified isoforms	506:542	three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation	506:594	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	3	28	theme	peptides	557:564	arg1	PTM					608:610	an unusual PTM	597:610	an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients	597:683	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	4	29	dep	aggregation	745:755	arg1	the					741:743	the	741:743	the	741:743	We discovered that O-glycans significantly affect both the aggregation and degradation of Aβ42.					
34841862	0	30	theme	Fibril	46:51	arg1	Polymorphs					53:62	New Fibril Polymorphs	42:62	New Fibril Polymorphs Vulnerable for Degradation	42:89	O-Glycosylation Induces Amyloid-β To Form New Fibril Polymorphs Vulnerable for Degradation.					
34841862	5	31	theme	fibril	913:918	arg1	stable					955:960	stable	955:960	stable	955:960	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	5	31	theme	fibril	913:918	arg1	structure					932:940	a new fibril polymorphic structure	907:940	a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme)	907:1037	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	5	32	theme	Aβ-degrading	985:996	arg1	enzymes					998:1004	Aβ-degrading enzymes	985:1004	Aβ-degrading enzymes (e.g., insulin-degrading enzyme)	985:1037	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	3	33	theme	unusual	600:606	arg1	isoforms					535:542	three homogeneously modified isoforms	506:542	three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation	506:594	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	3	33	theme	unusual	600:606	arg1	PTM					608:610	an unusual PTM	597:610	an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients	597:683	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	5	34	theme	polymorphic	920:930	arg1	stable					955:960	stable	955:960	stable	955:960	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	5	34	theme	polymorphic	920:930	arg1	structure					932:940	a new fibril polymorphic structure	907:940	a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme)	907:1037	By combining cryo-EM and various biochemical assays, we demonstrate that a Galβ1-3GalNAc modification redirects Aβ42 to form a new fibril polymorphic structure that is less stable and more vulnerable to Aβ-degrading enzymes (e.g., insulin-degrading enzyme).					
34841862	6	35	theme	powerful	1173:1180	arg1	tools					1195:1199	powerful experimental tools	1173:1199	powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity	1173:1308	Thus, beyond showing how particular O-glycosylation modifications affect Aβ42 aggregation at the molecular level, our study provides powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity.					
34841862	3	36	theme	cerebrospinal	642:654	arg1	samples					662:668	the cerebrospinal fluid samples	638:668	the cerebrospinal fluid samples of AD patients	638:683	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	1	37	theme	peptides	129:136	arg1	accumulation					98:109	Brain accumulation	92:109	Brain accumulation of amyloid-β (Aβ) peptides (resulting from a disrupted balance between biosynthesis and clearance)	92:208	Brain accumulation of amyloid-β (Aβ) peptides (resulting from a disrupted balance between biosynthesis and clearance) occurs during the progression of Alzheimer's disease (AD).					
34841862	3	38	theme	fluid	656:660	arg1	samples					662:668	the cerebrospinal fluid samples	638:668	the cerebrospinal fluid samples of AD patients	638:683	Here, we chemically synthesized three homogeneously modified isoforms of Aβ (1-42) peptides bearing Tyr10 O-glycosylation, an unusual PTM initially identified from the cerebrospinal fluid samples of AD patients.					
34841862	6	39	theme	further	1212:1218	arg1	investigations					1220:1233	further investigations	1212:1233	further investigations	1212:1233	Thus, beyond showing how particular O-glycosylation modifications affect Aβ42 aggregation at the molecular level, our study provides powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity.					
34841862	2	40	theme	Aβ	357:358	arg1	aggregation					360:370	Aβ aggregation	357:370	Aβ aggregation into fibrils	357:383	Aβ peptides have diverse posttranslational modifications (PTMs) that variously modulate Aβ aggregation into fibrils, but understanding the mechanistic roles of PTMs in these processes remains a challenge.					
34841862	6	41	theme	particular	1065:1074	arg1	modifications					1092:1104	particular O-glycosylation modifications	1065:1104	particular O-glycosylation modifications	1065:1104	Thus, beyond showing how particular O-glycosylation modifications affect Aβ42 aggregation at the molecular level, our study provides powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity.					
34841862	0	42	theme	Vulnerable	64:73	arg1	Polymorphs					53:62	New Fibril Polymorphs	42:62	New Fibril Polymorphs Vulnerable for Degradation	42:89	O-Glycosylation Induces Amyloid-β To Form New Fibril Polymorphs Vulnerable for Degradation.					
34841862	6	43	theme	Aβ42	1113:1116	arg1	aggregation					1118:1128	Aβ42 aggregation	1113:1128	Aβ42 aggregation	1113:1128	Thus, beyond showing how particular O-glycosylation modifications affect Aβ42 aggregation at the molecular level, our study provides powerful experimental tools to support further investigations about how PTMs affect Aβ42 fibril aggregation and AD-related neurotoxicity.					
32212687	4	0	used	used	795:798	arg2	DESs					784:787	DESs	784:787	DESs	784:787	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	4	0	used	used	795:798	arg2	friendly					736:743	environmentally friendly	720:743	environmentally friendly	720:743	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	4	0	used	used	795:798	arg2	solvents					774:781	recyclable deep eutectic solvents	749:781	recyclable deep eutectic solvents (DESs)	749:788	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	1	1	theme	materials	270:278	arg1	properties					248:257	mechanical properties	237:257	mechanical properties	237:257	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	1	theme	materials	270:278	arg1	functions					223:231	outstanding functions	211:231	outstanding functions	211:231	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	8	2	from	delivery	1951:1958	arg1	biomedicine					1963:1973	biomedicine	1963:1973	biomedicine	1963:1973	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	6	3	theme	%	1230:1230	arg1	yield					1210:1214	a recovery yield	1199:1214	a recovery yield of at least 92%	1199:1230	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	3	4	theme	silk	599:602	arg1	SNFs/SNWs					629:637	SNFs/SNWs	629:637	SNFs/SNWs	629:637	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32212687	3	4	theme	silk	599:602	arg1	nanofibers/nanowhiskers					604:626	silk nanofibers/nanowhiskers	599:626	silk nanofibers/nanowhiskers (SNFs/SNWs)	599:638	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32212687	8	5	theme	pretreatment	1704:1715	arg1	process					1717:1723	the DES pretreatment process	1696:1723	the DES pretreatment process	1696:1723	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	5	theme	pretreatment	1704:1715	arg1	promising					1728:1736	promising	1728:1736	promising	1728:1736	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	7	6	theme	SNWs	1428:1431	arg1	properties					1414:1423	the inherent amphoteric properties	1390:1423	the inherent amphoteric properties of SNWs	1390:1431	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	4	7	theme	environmentally	720:734	arg1	friendly					736:743	environmentally friendly	720:743	environmentally friendly	720:743	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	1	8	theme	most	176:179	arg1	contributors					191:202	one of the most important contributors	165:202	one of the most important contributors to the outstanding functions and mechanical properties of natural materials	165:278	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	8	theme	most	176:179	arg1	nanofibers					110:119	Native biopolymer nanofibers	92:119	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber)	92:159	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	4	9	with	silks	840:844	arg1	yield					879:883	high yield	874:883	high yield	874:883	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	2	10	theme	great	322:326	arg1	deal					328:331	A great deal	320:331	A great deal of top-down routes	320:350	To enhance the mechanical performance, A great deal of top-down routes have been reported to prepare biopolymers nanofibers/nanowhiskers that retaining their nanostructures.					
32212687	5	11	theme	OA/ChCl	928:934	arg1	SNWs					948:951	OA/ChCl DES treated SNWs	928:951	OA/ChCl DES treated SNWs	928:951	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	3	12	dep	downsizing	559:568	arg1	fibers					587:592	the natural silk fibers	570:592	direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs)	552:638	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32212687	5	13	theme	similar	896:902	arg1	diameter					904:911	similar diameter	896:911	similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs)	896:992	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	7	14	theme	double	1629:1634	arg1	properties					1647:1656	protein-endowed double adsorption properties	1613:1656	protein-endowed double adsorption properties	1613:1656	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	8	15	contain	have	1831:1834	arg2	materials					1864:1872	nanoscale materials	1854:1872	nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine	1854:1973	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	15	contain	have	1831:1834	arg2	prospects					1841:1849	good prospects	1836:1849	good prospects	1836:1849	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	15	contain	have	1831:1834	arg1	SNWs					1809:1812	the SNWs	1805:1812	the SNWs prepared via DES	1805:1829	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	3	16	theme	high	657:660	arg1	crystallinity					662:674	their high crystallinity	651:674	their high crystallinity	651:674	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32212687	8	17	theme	biopolymer	1761:1770	arg1	extraction					1785:1794	green and low-cost biopolymer nanomaterial extraction	1742:1794	green and low-cost biopolymer nanomaterial extraction	1742:1794	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	18	theme	drug	1946:1949	arg1	delivery					1951:1958	drug delivery	1946:1958	drug delivery in biomedicine	1946:1973	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	19	theme	nanomaterial	1772:1783	arg1	extraction					1785:1794	green and low-cost biopolymer nanomaterial extraction	1742:1794	green and low-cost biopolymer nanomaterial extraction	1742:1794	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	5	20	theme	CA/ChCl	968:974	arg1	SNWs					988:991	CA/ChCl DES treated SNWs	968:991	CA/ChCl DES treated SNWs	968:991	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	4	21	theme	deep	760:763	arg1	DESs					784:787	DESs	784:787	DESs	784:787	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	4	21	theme	deep	760:763	arg1	solvents					774:781	recyclable deep eutectic solvents	749:781	recyclable deep eutectic solvents (DESs)	749:788	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	5	22	theme	treated	940:946	arg1	SNWs					948:951	OA/ChCl DES treated SNWs	928:951	OA/ChCl DES treated SNWs	928:951	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	7	23	theme	enhanced	1560:1567	arg1	performance					1569:1579	enhanced performance	1560:1579	enhanced performance	1560:1579	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	6	24	theme	green	1314:1318	arg1	solvents					1320:1327	green solvents	1314:1327	green solvents for sustainable biopolymer nanomaterial extraction	1314:1378	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	6	24	theme	green	1314:1318	arg1	possibility					1291:1301	the possibility	1287:1301	the possibility of DESs	1287:1309	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	7	25	with	endowed	1585:1591	arg1	properties					1647:1656	protein-endowed double adsorption properties	1613:1656	protein-endowed double adsorption properties	1613:1656	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	1	26	theme	of	169:170	arg1	contributors					191:202	one of the most important contributors	165:202	one of the most important contributors to the outstanding functions and mechanical properties of natural materials	165:278	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	26	theme	of	169:170	arg1	nanofibers					110:119	Native biopolymer nanofibers	92:119	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber)	92:159	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	7	27	theme	membranes	1545:1553	arg1	preparation					1519:1529	preparation	1519:1529	preparation of coextruded membranes with enhanced performance	1519:1579	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	6	28	theme	DESs	1306:1309	arg1	solvents					1320:1327	green solvents	1314:1327	green solvents for sustainable biopolymer nanomaterial extraction	1314:1378	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	6	28	theme	DESs	1306:1309	arg1	possibility					1291:1301	the possibility	1287:1301	the possibility of DESs	1287:1309	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	5	29	theme	DES	1082:1084	arg1	SNWs					1094:1097	CA/ChCl DES treated SNWs	1074:1097	CA/ChCl DES treated SNWs	1074:1097	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	2	30	theme	mechanical	296:305	arg1	performance					307:317	the mechanical performance	292:317	the mechanical performance	292:317	To enhance the mechanical performance, A great deal of top-down routes have been reported to prepare biopolymers nanofibers/nanowhiskers that retaining their nanostructures.					
32212687	1	31	theme	Native	92:97	arg1	cellulose					122:130	cellulose	122:130	cellulose	122:130	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	31	theme	Native	92:97	arg1	nanofibers					110:119	Native biopolymer nanofibers	92:119	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber)	92:159	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	31	theme	Native	92:97	arg1	nanofiber					150:158	silk nanofiber	145:158	silk nanofiber	145:158	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	31	theme	Native	92:97	arg1	chitin					133:138	chitin	133:138	chitin	133:138	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	31	theme	Native	92:97	arg1	contributors					191:202	one of the most important contributors	165:202	one of the most important contributors to the outstanding functions and mechanical properties of natural materials	165:278	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	0	32	theme	Cellulose/Chitin	54:69	arg1	Membranes					81:89	Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes	15:89	Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes	15:89	Preparation of Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes.					
32212687	8	33	theme	nanoscale	1854:1862	arg1	prospects					1841:1849	good prospects	1836:1849	good prospects	1836:1849	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	33	theme	nanoscale	1854:1862	arg1	materials					1864:1872	nanoscale materials	1854:1872	nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine	1854:1973	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	5	34	theme	treated	980:986	arg1	SNWs					988:991	CA/ChCl DES treated SNWs	968:991	CA/ChCl DES treated SNWs	968:991	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	0	35	theme	Silk	15:18	arg1	Membranes					81:89	Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes	15:89	Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes	15:89	Preparation of Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes.					
32212687	8	36	from	materials	1864:1872	arg1	delivery					1951:1958	drug delivery	1946:1958	drug delivery in biomedicine	1946:1973	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	36	from	materials	1864:1872	arg1	field					1895:1899	the environmental field	1877:1899	the environmental field	1877:1899	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	36	from	materials	1864:1872	arg1	development					1908:1918	development	1908:1918	development of smart biomaterials	1908:1940	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	0	37	theme	Amphoteric	43:52	arg1	Membranes					81:89	Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes	15:89	Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes	15:89	Preparation of Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes.					
32212687	5	38	theme	natural	1128:1134	arg1	fibers					1141:1146	natural silk fibers	1128:1146	natural silk fibers	1128:1146	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	5	39	theme	OA/ChCl	1031:1037	arg1	SNWs					1051:1054	OA/ChCl DES treated SNWs	1031:1054	OA/ChCl DES treated SNWs	1031:1054	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	6	40	theme	biopolymer	1345:1354	arg1	extraction					1369:1378	sustainable biopolymer nanomaterial extraction	1333:1378	sustainable biopolymer nanomaterial extraction	1333:1378	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	7	41	theme	composite	1478:1486	arg1	SNWs					1488:1491	composite SNWs	1478:1491	composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance	1478:1579	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	5	42	theme	treated	1043:1049	arg1	SNWs					1051:1054	OA/ChCl DES treated SNWs	1031:1054	OA/ChCl DES treated SNWs	1031:1054	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	5	43	dep	diameter	904:911	arg1	nm					1067:1068	365 ± 200 nm	1057:1068	365 ± 200 nm for CA/ChCl DES treated SNWs	1057:1097	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	5	43	dep	diameter	904:911	arg1	nm					921:922	3.1-22 nm	914:922	3.1-22 nm for OA/ChCl DES treated SNWs	914:951	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	5	43	dep	diameter	904:911	arg1	nm					1024:1025	329 ± 140 nm	1014:1025	329 ± 140 nm for OA/ChCl DES treated SNWs	1014:1054	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	5	43	dep	diameter	904:911	arg1	nm					961:962	2.7-20 nm	954:962	2.7-20 nm for CA/ChCl DES treated SNWs	954:991	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	2	44	theme	top-down	336:343	arg1	routes					345:350	top-down routes	336:350	top-down routes	336:350	To enhance the mechanical performance, A great deal of top-down routes have been reported to prepare biopolymers nanofibers/nanowhiskers that retaining their nanostructures.					
32212687	3	45	from	advances	469:476	arg1	nanofibers/nanowhiskers					502:524	cellulose and chitin nanofibers/nanowhiskers	481:524	cellulose and chitin nanofibers/nanowhiskers	481:524	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32212687	6	46	theme	nanomaterial	1356:1367	arg1	extraction					1369:1378	sustainable biopolymer nanomaterial extraction	1333:1378	sustainable biopolymer nanomaterial extraction	1333:1378	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	3	47	theme	direct	552:557	arg1	downsizing					559:568	direct downsizing the natural silk fibers	552:592	direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs)	552:638	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32212687	1	48	theme	natural	262:268	arg1	materials					270:278	natural materials	262:278	natural materials	262:278	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	8	49	theme	environmental	1881:1893	arg1	field					1895:1899	the environmental field	1877:1899	the environmental field	1877:1899	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	7	50	theme	inherent	1394:1401	arg1	properties					1414:1423	the inherent amphoteric properties	1390:1423	the inherent amphoteric properties of SNWs	1390:1431	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	3	51	theme	silk	582:585	arg1	fibers					587:592	the natural silk fibers	570:592	direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs)	552:638	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32212687	8	52	theme	DES	1700:1702	arg1	process					1717:1723	the DES pretreatment process	1696:1723	the DES pretreatment process	1696:1723	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	52	theme	DES	1700:1702	arg1	promising					1728:1736	promising	1728:1736	promising	1728:1736	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	0	53	theme	Nanofiber	71:79	arg1	Membranes					81:89	Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes	15:89	Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes	15:89	Preparation of Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes.					
32212687	7	54	theme	amphoteric	1403:1412	arg1	properties					1414:1423	the inherent amphoteric properties	1390:1423	the inherent amphoteric properties of SNWs	1390:1431	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	7	55	with	SNWs	1488:1491	arg1	polymers					1506:1513	various polymers	1498:1513	various polymers	1498:1513	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	1	56	theme	important	181:189	arg1	contributors					191:202	one of the most important contributors	165:202	one of the most important contributors to the outstanding functions and mechanical properties of natural materials	165:278	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	56	theme	important	181:189	arg1	nanofibers					110:119	Native biopolymer nanofibers	92:119	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber)	92:159	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	7	57	theme	adsorption	1636:1645	arg1	properties					1647:1656	protein-endowed double adsorption properties	1613:1656	protein-endowed double adsorption properties	1613:1656	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	5	58	with	SNWs	886:889	arg1	diameter					904:911	similar diameter	896:911	similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs)	896:992	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	5	58	with	SNWs	886:889	arg1	length					1006:1011	contour length	998:1011	contour length	998:1011	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	8	59	theme	smart	1923:1927	arg1	biomaterials					1929:1940	smart biomaterials	1923:1940	smart biomaterials	1923:1940	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	7	60	theme	protein-endowed	1613:1627	arg1	properties					1647:1656	protein-endowed double adsorption properties	1613:1656	protein-endowed double adsorption properties	1613:1656	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	8	61	theme	green	1742:1746	arg1	extraction					1785:1794	green and low-cost biopolymer nanomaterial extraction	1742:1794	green and low-cost biopolymer nanomaterial extraction	1742:1794	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	1	62	theme	outstanding	211:221	arg1	functions					223:231	outstanding functions	211:231	outstanding functions	211:231	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	6	63	theme	recovery	1201:1208	arg1	yield					1210:1214	a recovery yield	1199:1214	a recovery yield of at least 92%	1199:1230	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	5	64	theme	CA/ChCl	1074:1080	arg1	SNWs					1094:1097	CA/ChCl DES treated SNWs	1074:1097	CA/ChCl DES treated SNWs	1074:1097	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	8	65	theme	low-cost	1752:1759	arg1	extraction					1785:1794	green and low-cost biopolymer nanomaterial extraction	1742:1794	green and low-cost biopolymer nanomaterial extraction	1742:1794	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	1	66	dep	nanofibers	110:119	arg1	cellulose					122:130	cellulose	122:130	cellulose	122:130	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	66	dep	nanofibers	110:119	arg1	nanofiber					150:158	silk nanofiber	145:158	silk nanofiber	145:158	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	66	dep	nanofibers	110:119	arg1	nanofibers					110:119	Native biopolymer nanofibers	92:119	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber)	92:159	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	66	dep	nanofibers	110:119	arg1	chitin					133:138	chitin	133:138	chitin	133:138	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	4	67	theme	silk	851:854	arg1	nanowhiskers					856:867	silk nanowhiskers	851:867	silk nanowhiskers	851:867	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	3	68	theme	sophisticated	680:692	arg1	structures					694:703	sophisticated structures	680:703	sophisticated structures	680:703	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32212687	7	69	with	membranes	1545:1553	arg1	performance					1569:1579	enhanced performance	1560:1579	enhanced performance	1560:1579	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	1	70	theme	silk	145:148	arg1	nanofiber					150:158	silk nanofiber	145:158	silk nanofiber	145:158	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	70	theme	silk	145:148	arg1	nanofibers					110:119	Native biopolymer nanofibers	92:119	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber)	92:159	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	6	71	theme	separated	1180:1188	arg1	DES					1190:1192	the separated DES	1176:1192	the separated DES with a recovery yield of at least 92%	1176:1230	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	6	72	theme	sustainable	1333:1343	arg1	extraction					1369:1378	sustainable biopolymer nanomaterial extraction	1333:1378	sustainable biopolymer nanomaterial extraction	1333:1378	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	5	73	theme	±	1061:1061	arg1	nm					1067:1068	365 ± 200 nm	1057:1068	365 ± 200 nm for CA/ChCl DES treated SNWs	1057:1097	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	5	73	theme	±	1061:1061	arg1	nm					1024:1025	329 ± 140 nm	1014:1025	329 ± 140 nm for OA/ChCl DES treated SNWs	1014:1054	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	5	74	theme	DES	936:938	arg1	SNWs					948:951	OA/ChCl DES treated SNWs	928:951	OA/ChCl DES treated SNWs	928:951	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	4	75	theme	recyclable	749:758	arg1	DESs					784:787	DESs	784:787	DESs	784:787	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	4	75	theme	recyclable	749:758	arg1	solvents					774:781	recyclable deep eutectic solvents	749:781	recyclable deep eutectic solvents (DESs)	749:788	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	5	76	theme	individual	1103:1112	arg1	nanofibers					1114:1123	individual nanofibers	1103:1123	individual nanofibers in natural silk fibers	1103:1146	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	7	77	theme	coextruded	1534:1543	arg1	membranes					1545:1553	coextruded membranes	1534:1553	coextruded membranes with enhanced performance	1534:1579	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	4	78	theme	eutectic	765:772	arg1	DESs					784:787	DESs	784:787	DESs	784:787	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	4	78	theme	eutectic	765:772	arg1	solvents					774:781	recyclable deep eutectic solvents	749:781	recyclable deep eutectic solvents (DESs)	749:788	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	6	79	with	DES	1190:1192	arg1	yield					1210:1214	a recovery yield	1199:1214	a recovery yield of at least 92%	1199:1230	In addition, the separated DES with a recovery yield of at least 92% could be reused four times to produce SNWs, indicating the possibility of DESs as green solvents for sustainable biopolymer nanomaterial extraction.					
32212687	5	80	theme	contour	998:1004	arg1	length					1006:1011	contour length	998:1011	contour length	998:1011	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	5	81	theme	treated	1086:1092	arg1	SNWs					1094:1097	CA/ChCl DES treated SNWs	1074:1097	CA/ChCl DES treated SNWs	1074:1097	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	1	82	theme	biopolymer	99:108	arg1	cellulose					122:130	cellulose	122:130	cellulose	122:130	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	82	theme	biopolymer	99:108	arg1	nanofibers					110:119	Native biopolymer nanofibers	92:119	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber)	92:159	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	82	theme	biopolymer	99:108	arg1	nanofiber					150:158	silk nanofiber	145:158	silk nanofiber	145:158	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	82	theme	biopolymer	99:108	arg1	chitin					133:138	chitin	133:138	chitin	133:138	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	82	theme	biopolymer	99:108	arg1	contributors					191:202	one of the most important contributors	165:202	one of the most important contributors to the outstanding functions and mechanical properties of natural materials	165:278	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	4	83	theme	natural	832:838	arg1	silks					840:844	natural silks	832:844	natural silks into silk nanowhiskers with high yield	832:883	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	3	84	theme	cellulose	481:489	arg1	nanofibers/nanowhiskers					502:524	cellulose and chitin nanofibers/nanowhiskers	481:524	cellulose and chitin nanofibers/nanowhiskers	481:524	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32212687	8	85	theme	good	1836:1839	arg1	prospects					1841:1849	good prospects	1836:1849	good prospects	1836:1849	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	85	theme	good	1836:1839	arg1	materials					1864:1872	nanoscale materials	1854:1872	nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine	1854:1973	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	5	86	theme	DES	976:978	arg1	SNWs					988:991	CA/ChCl DES treated SNWs	968:991	CA/ChCl DES treated SNWs	968:991	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	2	87	theme	biopolymers	382:392	arg1	nanofibers/nanowhiskers					394:416	biopolymers nanofibers/nanowhiskers	382:416	biopolymers nanofibers/nanowhiskers	382:416	To enhance the mechanical performance, A great deal of top-down routes have been reported to prepare biopolymers nanofibers/nanowhiskers that retaining their nanostructures.					
32212687	0	88	theme	Nanowhisker-Composited	20:41	arg1	Membranes					81:89	Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes	15:89	Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes	15:89	Preparation of Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes.					
32212687	5	89	from	nanofibers	1114:1123	arg1	fibers					1141:1146	natural silk fibers	1128:1146	natural silk fibers	1128:1146	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	8	90	from	development	1908:1918	arg1	biomedicine					1963:1973	biomedicine	1963:1973	biomedicine	1963:1973	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	4	91	theme	high	874:877	arg1	yield					879:883	high yield	874:883	high yield	874:883	In this work, environmentally friendly and recyclable deep eutectic solvents (DESs) were used to direct pretreat and downsize natural silks into silk nanowhiskers with high yield.					
32212687	3	92	theme	chitin	495:500	arg1	nanofibers/nanowhiskers					502:524	cellulose and chitin nanofibers/nanowhiskers	481:524	cellulose and chitin nanofibers/nanowhiskers	481:524	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32212687	0	93	theme	Membranes	81:89	arg1	Preparation					0:10	Preparation	0:10	Preparation of Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes.	0:90	Preparation of Silk Nanowhisker-Composited Amphoteric Cellulose/Chitin Nanofiber Membranes.					
32212687	5	94	theme	silk	1136:1139	arg1	fibers					1141:1146	natural silk fibers	1128:1146	natural silk fibers	1128:1146	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	7	95	theme	various	1498:1504	arg1	polymers					1506:1513	various polymers	1498:1513	various polymers	1498:1513	Based on the inherent amphoteric properties of SNWs, multicompatibility was explored to facilely composite SNWs with various polymers for preparation of coextruded membranes with enhanced performance and endowed the composites with protein-endowed double adsorption properties.					
32212687	5	96	theme	DES	1039:1041	arg1	SNWs					1051:1054	OA/ChCl DES treated SNWs	1031:1054	OA/ChCl DES treated SNWs	1031:1054	SNWs with similar diameter (3.1-22 nm for OA/ChCl DES treated SNWs, 2.7-20 nm for CA/ChCl DES treated SNWs) and contour length (329 ± 140 nm for OA/ChCl DES treated SNWs, 365 ± 200 nm for CA/ChCl DES treated SNWs) to individual nanofibers in natural silk fibers were obtained.					
32212687	8	97	theme	biomaterials	1929:1940	arg1	delivery					1951:1958	drug delivery	1946:1958	drug delivery in biomedicine	1946:1973	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	8	97	theme	biomaterials	1929:1940	arg1	development					1908:1918	development	1908:1918	development of smart biomaterials	1908:1940	Overall, this work demonstrated that the DES pretreatment process is promising for green and low-cost biopolymer nanomaterial extraction and that the SNWs prepared via DES have good prospects as nanoscale materials in the environmental field and in development of smart biomaterials and drug delivery in biomedicine.					
32212687	2	98	theme	routes	345:350	arg1	deal					328:331	A great deal	320:331	A great deal of top-down routes	320:350	To enhance the mechanical performance, A great deal of top-down routes have been reported to prepare biopolymers nanofibers/nanowhiskers that retaining their nanostructures.					
32212687	1	99	dep	functions	223:231	arg1	the					207:209	the	207:209	the	207:209	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	1	100	theme	mechanical	237:246	arg1	properties					248:257	mechanical properties	237:257	mechanical properties	237:257	Native biopolymer nanofibers (cellulose, chitin, and silk nanofiber) are one of the most important contributors to the outstanding functions and mechanical properties of natural materials.					
32212687	3	101	theme	natural	574:580	arg1	fibers					587:592	the natural silk fibers	570:592	direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs)	552:638	Compared with advances in cellulose and chitin nanofibers/nanowhiskers, it remains difficult for direct downsizing the natural silk fibers into silk nanofibers/nanowhiskers (SNFs/SNWs) because of their high crystallinity and sophisticated structures.					
32397611	2	0	theme	agricultural	454:465	arg1	crop					467:470	an important agricultural crop	441:470	an important agricultural crop	441:470	In this category, barley straw is an important agricultural crop, due to its abundance and availability.					
32397611	2	0	theme	agricultural	454:465	arg1	straw					432:436	barley straw	425:436	barley straw	425:436	In this category, barley straw is an important agricultural crop, due to its abundance and availability.					
32397611	8	1	dep	the	1155:1157	arg1	elevate					1159:1165	elevate	1159:1165	elevate increments in the flexural strength	1159:1201	A good dispersion of the reinforcement inside the plastic was achieved contributing to the elevate increments in the flexural strength.					
32397611	3	2	theme	thermomechanical	571:586	arg1	process					588:594	thermomechanical process	571:594	thermomechanical process	571:594	In the current investigation, the residue was submitted to thermomechanical process for fiber extraction and individualization.					
32397611	5	3	theme	biobased	885:892	arg1	BioPE					908:912	BioPE	908:912	BioPE	908:912	Therefore, fully biobased composites were fabricated using barley fibers and a biobased polyethylene (BioPE) as polymer matrix.					
32397611	5	3	theme	biobased	885:892	arg1	polyethylene					894:905	a biobased polyethylene	883:905	a biobased polyethylene (BioPE)	883:913	Therefore, fully biobased composites were fabricated using barley fibers and a biobased polyethylene (BioPE) as polymer matrix.					
32397611	10	4	theme	reinforcement	1220:1232	arg1	reinforcement					1220:1232	reinforcement	1220:1232	reinforcement	1220:1232	% of reinforcement, an increment in the flexural strength of about 147% was attained.					
32397611	10	4	theme	reinforcement	1220:1232	arg1	increment					1238:1246	an increment	1235:1246	an increment in the flexural strength of about 147%	1235:1285	% of reinforcement, an increment in the flexural strength of about 147% was attained.					
32397611	10	4	theme	reinforcement	1220:1232	arg1	%					1215:1215	%	1215:1215	% of reinforcement, an increment in the flexural strength of about 147%	1215:1285	% of reinforcement, an increment in the flexural strength of about 147% was attained.					
32397611	12	5	theme	micromechanical	1451:1465	arg1	analysis					1467:1474	The micromechanical analysis	1447:1474	The micromechanical analysis	1447:1474	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	0	6	theme	Polyethylene	70:81	arg1	Composites					83:92	Fully Biobased Polyethylene Composites	55:92	Fully Biobased Polyethylene Composites	55:92	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	8	7	from	increments	1167:1176	arg1	strength					1194:1201	the flexural strength	1181:1201	the flexural strength	1181:1201	A good dispersion of the reinforcement inside the plastic was achieved contributing to the elevate increments in the flexural strength.					
32397611	1	8	from	sources	293:299	arg1	replacement					304:314	replacement	304:314	replacement of wood feedstock for some applications such as composite materials and energy production	304:404	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	1	9	from	Awareness	147:155	arg1	deforestation					160:172	deforestation	160:172	deforestation	160:172	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	1	9	from	Awareness	147:155	arg1	biodiversity					213:224	biodiversity	213:224	biodiversity	213:224	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	1	9	from	Awareness	147:155	arg1	warming					237:243	global warming	230:243	global warming	230:243	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	13	10	theme	biobased	1789:1796	arg1	composites					1806:1815	fully biobased polymer composites	1783:1815	fully biobased polymer composites	1783:1815	Overall, the investigation brightness on the potential use of barley straw residues as reinforcement in fully biobased polymer composites.					
32397611	13	11	theme	polymer	1798:1804	arg1	composites					1806:1815	fully biobased polymer composites	1783:1815	fully biobased polymer composites	1783:1815	Overall, the investigation brightness on the potential use of barley straw residues as reinforcement in fully biobased polymer composites.					
32397611	1	12	theme	wood	319:322	arg1	feedstock					324:332	wood feedstock	319:332	wood feedstock for some applications such as composite materials	319:382	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	12	13	theme	natural	1653:1659	arg1	composites					1667:1676	other natural fiber composites	1647:1676	other natural fiber composites	1647:1676	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	1	14	theme	feedstock	324:332	arg1	replacement					304:314	replacement	304:314	replacement of wood feedstock for some applications such as composite materials and energy production	304:404	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	12	15	with	line	1637:1640	arg1	composites					1667:1676	other natural fiber composites	1647:1676	other natural fiber composites	1647:1676	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	13	16	theme	residues	1754:1761	arg1	use					1734:1736	the potential use	1720:1736	the potential use of barley straw residues as reinforcement in fully biobased polymer composites	1720:1815	Overall, the investigation brightness on the potential use of barley straw residues as reinforcement in fully biobased polymer composites.					
32397611	0	17	theme	Micro	105:109	arg1	Mechanics					111:119	Micro Mechanics	105:119	Micro Mechanics	105:119	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	12	18	theme	factors	1593:1599	arg1	strength					1525:1532	the intrinsic flexural strength	1502:1532	the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors	1502:1599	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	4	19	theme	fibers	759:764	arg1	use					746:748	the potential use	732:748	the potential use of these fibers as reinforcement	732:781	The high content of holocellulose combined with their relatively high aspect ratio inspires the potential use of these fibers as reinforcement in plastic composites.					
32397611	5	20	theme	barley	865:870	arg1	fibers					872:877	barley fibers	865:877	barley fibers	865:877	Therefore, fully biobased composites were fabricated using barley fibers and a biobased polyethylene (BioPE) as polymer matrix.					
32397611	2	21	theme	barley	425:430	arg1	crop					467:470	an important agricultural crop	441:470	an important agricultural crop	441:470	In this category, barley straw is an important agricultural crop, due to its abundance and availability.					
32397611	2	21	theme	barley	425:430	arg1	straw					432:436	barley straw	425:436	barley straw	425:436	In this category, barley straw is an important agricultural crop, due to its abundance and availability.					
32397611	1	22	from	use	268:270	arg1	replacement					304:314	replacement	304:314	replacement of wood feedstock for some applications such as composite materials and energy production	304:404	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	13	23	theme	straw	1748:1752	arg1	residues					1754:1761	barley straw residues	1741:1761	barley straw residues	1741:1761	Overall, the investigation brightness on the potential use of barley straw residues as reinforcement in fully biobased polymer composites.					
32397611	12	24	theme	coupling	1584:1591	arg1	factors					1593:1599	coupling factors	1584:1599	coupling factors	1584:1599	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	2	25	theme	important	444:452	arg1	crop					467:470	an important agricultural crop	441:470	an important agricultural crop	441:470	In this category, barley straw is an important agricultural crop, due to its abundance and availability.					
32397611	2	25	theme	important	444:452	arg1	straw					432:436	barley straw	425:436	barley straw	425:436	In this category, barley straw is an important agricultural crop, due to its abundance and availability.					
32397611	11	26	theme	fibers	1330:1335	arg1	contribution					1310:1321	The mean contribution	1301:1321	The mean contribution of the fibers to the flexural strength	1301:1360	The mean contribution of the fibers to the flexural strength was assessed by means of a fiber flexural strength factor, reaching a value of 91.4.					
32397611	5	27	theme	polymer	918:924	arg1	matrix					926:931	polymer matrix	918:931	polymer matrix	918:931	Therefore, fully biobased composites were fabricated using barley fibers and a biobased polyethylene (BioPE) as polymer matrix.					
32397611	1	28	theme	forest	175:180	arg1	degradation					182:192	forest degradation	175:192	forest degradation	175:192	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	8	29	theme	good	1070:1073	arg1	dispersion					1075:1084	A good dispersion	1068:1084	A good dispersion of the reinforcement inside the plastic	1068:1124	A good dispersion of the reinforcement inside the plastic was achieved contributing to the elevate increments in the flexural strength.					
32397611	12	30	theme	fibers	1541:1546	arg1	strength					1525:1532	the intrinsic flexural strength	1502:1532	the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors	1502:1599	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	13	31	theme	potential	1724:1732	arg1	use					1734:1736	the potential use	1720:1736	the potential use of barley straw residues as reinforcement in fully biobased polymer composites	1720:1815	Overall, the investigation brightness on the potential use of barley straw residues as reinforcement in fully biobased polymer composites.					
32397611	12	32	theme	other	1647:1651	arg1	composites					1667:1676	other natural fiber composites	1647:1676	other natural fiber composites	1647:1676	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	11	33	theme	flexural	1395:1402	arg1	factor					1413:1418	a fiber flexural strength factor	1387:1418	a fiber flexural strength factor	1387:1418	The mean contribution of the fibers to the flexural strength was assessed by means of a fiber flexural strength factor, reaching a value of 91.4.					
32397611	4	34	theme	high	644:647	arg1	content					649:655	The high content	640:655	The high content of holocellulose combined with their relatively high aspect ratio	640:721	The high content of holocellulose combined with their relatively high aspect ratio inspires the potential use of these fibers as reinforcement in plastic composites.					
32397611	0	35	theme	Straw	22:26	arg1	Fibers					28:33	Barley Straw Fibers	15:33	Barley Straw Fibers as Reinforcement	15:50	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	7	36	theme	materials	1044:1052	arg1	properties					1026:1035	the flexural properties	1013:1035	the flexural properties of the materials	1013:1052	As for material performance, the flexural properties of the materials were studied.					
32397611	4	37	theme	potential	736:744	arg1	use					746:748	the potential use	732:748	the potential use of these fibers as reinforcement	732:781	The high content of holocellulose combined with their relatively high aspect ratio inspires the potential use of these fibers as reinforcement in plastic composites.					
32397611	11	38	theme	strength	1404:1411	arg1	factor					1413:1418	a fiber flexural strength factor	1387:1418	a fiber flexural strength factor	1387:1418	The mean contribution of the fibers to the flexural strength was assessed by means of a fiber flexural strength factor, reaching a value of 91.4.					
32397611	0	39	theme	Barley	15:20	arg1	Fibers					28:33	Barley Straw Fibers	15:33	Barley Straw Fibers as Reinforcement	15:50	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	13	40	from	brightness	1706:1715	arg1	use					1734:1736	the potential use	1720:1736	the potential use of barley straw residues as reinforcement in fully biobased polymer composites	1720:1815	Overall, the investigation brightness on the potential use of barley straw residues as reinforcement in fully biobased polymer composites.					
32397611	3	41	theme	current	519:525	arg1	investigation					527:539	the current investigation	515:539	the current investigation	515:539	In the current investigation, the residue was submitted to thermomechanical process for fiber extraction and individualization.					
32397611	13	42	theme	barley	1741:1746	arg1	residues					1754:1761	barley straw residues	1741:1761	barley straw residues	1741:1761	Overall, the investigation brightness on the potential use of barley straw residues as reinforcement in fully biobased polymer composites.					
32397611	5	43	theme	biobased	823:830	arg1	composites					832:841	fully biobased composites	817:841	fully biobased composites	817:841	Therefore, fully biobased composites were fabricated using barley fibers and a biobased polyethylene (BioPE) as polymer matrix.					
32397611	11	44	theme	fiber	1389:1393	arg1	factor					1413:1418	a fiber flexural strength factor	1387:1418	a fiber flexural strength factor	1387:1418	The mean contribution of the fibers to the flexural strength was assessed by means of a fiber flexural strength factor, reaching a value of 91.4.					
32397611	0	45	theme	Flexural	128:135	arg1	Strength					137:144	the Flexural Strength	124:144	the Flexural Strength	124:144	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	10	46	from	increment	1238:1246	arg1	strength					1264:1271	the flexural strength	1251:1271	the flexural strength of about 147%	1251:1285	% of reinforcement, an increment in the flexural strength of about 147% was attained.					
32397611	10	47	theme	flexural	1255:1262	arg1	strength					1264:1271	the flexural strength	1251:1271	the flexural strength of about 147%	1251:1285	% of reinforcement, an increment in the flexural strength of about 147% was attained.					
32397611	0	48	theme	Fibers	28:33	arg1	Feasibility					0:10	Feasibility	0:10	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.	0:145	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	12	49	theme	flexural	1516:1523	arg1	strength					1525:1532	the intrinsic flexural strength	1502:1532	the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors	1502:1599	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	8	50	theme	flexural	1185:1192	arg1	strength					1194:1201	the flexural strength	1181:1201	the flexural strength	1181:1201	A good dispersion of the reinforcement inside the plastic was achieved contributing to the elevate increments in the flexural strength.					
32397611	1	51	theme	composite	364:372	arg1	materials					374:382	composite materials	364:382	composite materials	364:382	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	11	52	theme	flexural	1344:1351	arg1	strength					1353:1360	the flexural strength	1340:1360	the flexural strength	1340:1360	The mean contribution of the fibers to the flexural strength was assessed by means of a fiber flexural strength factor, reaching a value of 91.4.					
32397611	7	53	theme	material	991:998	arg1	performance					1000:1010	material performance	991:1010	material performance	991:1010	As for material performance, the flexural properties of the materials were studied.					
32397611	2	54	theme	due	473:475	arg1	crop					467:470	an important agricultural crop	441:470	an important agricultural crop	441:470	In this category, barley straw is an important agricultural crop, due to its abundance and availability.					
32397611	2	54	theme	due	473:475	arg1	straw					432:436	barley straw	425:436	barley straw	425:436	In this category, barley straw is an important agricultural crop, due to its abundance and availability.					
32397611	11	55	theme	mean	1305:1308	arg1	contribution					1310:1321	The mean contribution	1301:1321	The mean contribution of the fibers to the flexural strength	1301:1360	The mean contribution of the fibers to the flexural strength was assessed by means of a fiber flexural strength factor, reaching a value of 91.4.					
32397611	1	56	theme	alternative	275:285	arg1	sources					293:299	alternative fiber sources	275:299	alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production	275:404	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	7	57	theme	flexural	1017:1024	arg1	properties					1026:1035	the flexural properties	1013:1035	the flexural properties of the materials	1013:1052	As for material performance, the flexural properties of the materials were studied.					
32397611	2	58	from	crop	467:470	arg1	category					415:422	this category	410:422	this category	410:422	In this category, barley straw is an important agricultural crop, due to its abundance and availability.					
32397611	1	59	from	impact	203:208	arg1	deforestation					160:172	deforestation	160:172	deforestation	160:172	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	1	59	from	impact	203:208	arg1	biodiversity					213:224	biodiversity	213:224	biodiversity	213:224	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	1	59	from	impact	203:208	arg1	warming					237:243	global warming	230:243	global warming	230:243	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	12	60	theme	fiber	1661:1665	arg1	composites					1667:1676	other natural fiber composites	1647:1676	other natural fiber composites	1647:1676	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	1	61	from	replacement	304:314	arg1	use					268:270	the use	264:270	the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production	264:404	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	1	62	theme	fiber	287:291	arg1	sources					293:299	alternative fiber sources	275:299	alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production	275:404	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	0	63	theme	Strength	137:144	arg1	Macro					95:99	Macro	95:99	Macro	95:99	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	0	63	theme	Strength	137:144	arg1	Mechanics					111:119	Micro Mechanics	105:119	Micro Mechanics	105:119	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	10	64	theme	%	1285:1285	arg1	strength					1264:1271	the flexural strength	1251:1271	the flexural strength of about 147%	1251:1285	% of reinforcement, an increment in the flexural strength of about 147% was attained.					
32397611	3	65	theme	fiber	600:604	arg1	extraction					606:615	fiber extraction	600:615	fiber extraction	600:615	In the current investigation, the residue was submitted to thermomechanical process for fiber extraction and individualization.					
32397611	1	66	theme	sources	293:299	arg1	use					268:270	the use	264:270	the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production	264:404	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	8	67	theme	reinforcement	1093:1105	arg1	dispersion					1075:1084	A good dispersion	1068:1084	A good dispersion of the reinforcement inside the plastic	1068:1124	A good dispersion of the reinforcement inside the plastic was achieved contributing to the elevate increments in the flexural strength.					
32397611	1	68	theme	energy	388:393	arg1	production					395:404	energy production	388:404	energy production	388:404	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	13	69	from	reinforcement	1766:1778	arg1	composites					1806:1815	fully biobased polymer composites	1783:1815	fully biobased polymer composites	1783:1815	Overall, the investigation brightness on the potential use of barley straw residues as reinforcement in fully biobased polymer composites.					
32397611	4	70	theme	aspect	710:715	arg1	ratio					717:721	their relatively high aspect ratio	688:721	their relatively high aspect ratio	688:721	The high content of holocellulose combined with their relatively high aspect ratio inspires the potential use of these fibers as reinforcement in plastic composites.					
32397611	1	71	theme	production	395:404	arg1	replacement					304:314	replacement	304:314	replacement of wood feedstock for some applications such as composite materials and energy production	304:404	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	12	72	dep	700	1571:1573	arg1	to					1561:1562	to	1561:1562	to	1561:1562	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	12	73	theme	intrinsic	1506:1514	arg1	strength					1525:1532	the intrinsic flexural strength	1502:1532	the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors	1502:1599	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	4	74	theme	high	705:708	arg1	ratio					717:721	their relatively high aspect ratio	688:721	their relatively high aspect ratio	688:721	The high content of holocellulose combined with their relatively high aspect ratio inspires the potential use of these fibers as reinforcement in plastic composites.					
32397611	12	75	theme	strength	1525:1532	arg1	prediction					1488:1497	the prediction	1484:1497	the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors	1484:1599	The micromechanical analysis allowed the prediction of the intrinsic flexural strength of the fibers, arriving up to around 700 MPa, and coupling factors between 0.18 and 0.19, which are in line with other natural fiber composites.					
32397611	0	76	theme	Biobased	61:68	arg1	Composites					83:92	Fully Biobased Polyethylene Composites	55:92	Fully Biobased Polyethylene Composites	55:92	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	4	77	theme	plastic	786:792	arg1	composites					794:803	plastic composites	786:803	plastic composites	786:803	The high content of holocellulose combined with their relatively high aspect ratio inspires the potential use of these fibers as reinforcement in plastic composites.					
32397611	1	78	from	degradation	182:192	arg1	deforestation					160:172	deforestation	160:172	deforestation	160:172	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	1	78	from	degradation	182:192	arg1	biodiversity					213:224	biodiversity	213:224	biodiversity	213:224	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	1	78	from	degradation	182:192	arg1	warming					237:243	global warming	230:243	global warming	230:243	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
32397611	4	79	theme	holocellulose	660:672	arg1	content					649:655	The high content	640:655	The high content of holocellulose combined with their relatively high aspect ratio	640:721	The high content of holocellulose combined with their relatively high aspect ratio inspires the potential use of these fibers as reinforcement in plastic composites.					
32397611	0	80	dep	Feasibility	0:10	arg1	Macro					95:99	Macro	95:99	Macro	95:99	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	0	80	dep	Feasibility	0:10	arg1	Mechanics					111:119	Micro Mechanics	105:119	Micro Mechanics	105:119	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	0	81	from	Feasibility	0:10	arg1	Composites					83:92	Fully Biobased Polyethylene Composites	55:92	Fully Biobased Polyethylene Composites	55:92	Feasibility of Barley Straw Fibers as Reinforcement in Fully Biobased Polyethylene Composites: Macro and Micro Mechanics of the Flexural Strength.					
32397611	11	82	theme	91.4	1441:1444	arg1	value					1432:1436	a value	1430:1436	a value of 91.4	1430:1444	The mean contribution of the fibers to the flexural strength was assessed by means of a fiber flexural strength factor, reaching a value of 91.4.					
32397611	1	83	theme	global	230:235	arg1	warming					237:243	global warming	230:243	global warming	230:243	Awareness on deforestation, forest degradation, and its impact on biodiversity and global warming, is giving rise to the use of alternative fiber sources in replacement of wood feedstock for some applications such as composite materials and energy production.					
34543117	2	0	with	niche	364:368	arg1	induction					389:397	massive mucus induction	375:397	massive mucus induction	375:397	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	8	1	dep	B.	1690:1691	arg1	hyodysenteriae					1693:1706	hyodysenteriae	1693:1706	hyodysenteriae	1693:1706	The results emphasize the role of sialic acids in governing B. hyodysenteriae interactions with its host, which may open perspectives for therapeutic strategies.					
34543117	5	2	from	pigs	1066:1069	arg1	mucins					1054:1059	mucins	1054:1059	mucins from pigs with SD	1054:1077	Our results show that binding to mucins appears to be specific to B. hyodysenteriae, and within this species, the binding ability to mucins varies between strains/isolates, increases for mucins from pigs with SD, and is associated with sialic acid epitopes on mucins.					
34543117	7	3	theme	cluster	1331:1337	arg1	OPLS-DA					1419:1425	OPLS-DA	1419:1425	OPLS-DA	1419:1425	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	3	theme	cluster	1331:1337	arg1	analysis					1339:1346	hierarchical cluster analysis	1318:1346	hierarchical cluster analysis	1318:1346	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	6	4	theme	hyodysenteriae	1153:1166	arg1	8dII					1175:1178	B. hyodysenteriae strain 8dII	1150:1178	B. hyodysenteriae strain 8dII	1150:1178	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	1	5	theme	economic	257:264	arg1	impact					266:271	an economic impact	254:271	an economic impact on the swine industry	254:293	Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry.					
34543117	3	6	theme	B.	522:523	arg1	strain					540:545	a B. hyodysenteriae strain	520:545	a B. hyodysenteriae strain	520:545	We previously determined that a B. hyodysenteriae strain binds to colon mucins in a manner that differs between pigs and mucin types.					
34543117	7	7	theme	orthogonal	1352:1361	arg1	projections					1363:1373	orthogonal projections	1352:1373	orthogonal projections to latent structures	1352:1394	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	2	8	theme	B.	296:297	arg1	infection					314:322	B. hyodysenteriae infection	296:322	B. hyodysenteriae infection	296:322	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	8	9	theme	acids	1671:1675	arg1	role					1656:1659	the role	1652:1659	the role of sialic acids in governing B. hyodysenteriae interactions with its host, which may open perspectives for therapeutic strategies	1652:1789	The results emphasize the role of sialic acids in governing B. hyodysenteriae interactions with its host, which may open perspectives for therapeutic strategies.					
34543117	4	10	dep	level	776:780	arg1	hampsonii					828:836	B. hampsonii	825:836	B. hampsonii	825:836	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	10	dep	level	776:780	arg1	intermedia					846:855	B. intermedia	843:855	B. intermedia	843:855	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	10	dep	level	776:780	arg1	innocens					786:793	B. innocens	783:793	B. innocens	783:793	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	10	dep	level	776:780	arg1	pilosicoli					799:808	B. pilosicoli	796:808	B. pilosicoli	796:808	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	10	dep	level	776:780	arg1	murdochii					814:822	B. murdochii	811:822	B. murdochii	811:822	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	7	11	theme	good	1562:1565	arg1	predictors					1567:1576	good predictors	1562:1576	good predictors of B. hyodysenteriae strain 8dII infection in pigs	1562:1627	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	11	theme	good	1562:1565	arg1	structures					1524:1533	sialic acid-containing structures	1501:1533	sialic acid-containing structures in the mucin O-glycome	1501:1556	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	2	12	from	mucus	483:487	arg1	number					434:439	the number	430:439	the number of B. hyodysenteriae binding sites in the mucus	430:487	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	6	13	theme	glycosylation	1197:1209	arg1	shift					1245:1249	a shift	1243:1249	a shift in sialic acid-containing structures	1243:1286	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	6	13	theme	glycosylation	1197:1209	arg1	changes					1211:1217	mucin glycosylation changes	1191:1217	mucin glycosylation changes	1191:1217	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	3	14	dep	B.	522:523	arg1	hyodysenteriae					525:538	hyodysenteriae	525:538	hyodysenteriae	525:538	We previously determined that a B. hyodysenteriae strain binds to colon mucins in a manner that differs between pigs and mucin types.					
34543117	7	15	from	infection	1611:1619	arg1	pigs					1624:1627	pigs	1624:1627	pigs	1624:1627	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	5	16	from	epitopes	1115:1122	arg1	mucins					1127:1132	mucins	1127:1132	mucins	1127:1132	Our results show that binding to mucins appears to be specific to B. hyodysenteriae, and within this species, the binding ability to mucins varies between strains/isolates, increases for mucins from pigs with SD, and is associated with sialic acid epitopes on mucins.					
34543117	7	17	dep	analysis	1339:1346	arg1	analysis					1409:1416	discriminant analysis	1396:1416	discriminant analysis	1396:1416	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	18	from	pigs	1624:1627	arg1	predictors					1567:1576	good predictors	1562:1576	good predictors of B. hyodysenteriae strain 8dII infection in pigs	1562:1627	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	18	from	pigs	1624:1627	arg1	structures					1524:1533	sialic acid-containing structures	1501:1533	sialic acid-containing structures in the mucin O-glycome	1501:1556	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	19	theme	sialic	1501:1506	arg1	predictors					1567:1576	good predictors	1562:1576	good predictors of B. hyodysenteriae strain 8dII infection in pigs	1562:1627	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	19	theme	sialic	1501:1506	arg1	structures					1524:1533	sialic acid-containing structures	1501:1533	sialic acid-containing structures in the mucin O-glycome	1501:1556	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	6	20	theme	sialic	1254:1259	arg1	structures					1277:1286	sialic acid-containing structures	1254:1286	sialic acid-containing structures	1254:1286	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	2	21	dep	B.	444:445	arg1	hyodysenteriae					447:460	hyodysenteriae	447:460	hyodysenteriae	447:460	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	0	22	dep	Specific	121:128	arg1	Species					113:119	Species	113:119	Species	113:119	Brachyspira Species Avidity to Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection Is Species Specific and Varies between Strains.					
34543117	7	23	theme	glycans	1488:1494	arg1	abundances					1451:1460	the relative abundances	1438:1460	the relative abundances of sialic acid-containing glycans	1438:1494	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	2	24	from	number	434:439	arg1	mucus					483:487	the mucus	479:487	the mucus	479:487	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	0	25	theme	hyodysenteriae	85:98	arg1	Infection					100:108	Brachyspira hyodysenteriae Infection	73:108	Brachyspira hyodysenteriae Infection	73:108	Brachyspira Species Avidity to Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection Is Species Specific and Varies between Strains.					
34543117	7	26	theme	sialic	1465:1470	arg1	glycans					1488:1494	sialic acid-containing glycans	1465:1494	sialic acid-containing glycans	1465:1494	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	2	27	theme	mucus	383:387	arg1	induction					389:397	massive mucus induction	375:397	massive mucus induction	375:397	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	5	28	theme	sialic	1103:1108	arg1	epitopes					1115:1122	sialic acid epitopes	1103:1122	sialic acid epitopes on mucins	1103:1132	Our results show that binding to mucins appears to be specific to B. hyodysenteriae, and within this species, the binding ability to mucins varies between strains/isolates, increases for mucins from pigs with SD, and is associated with sialic acid epitopes on mucins.					
34543117	7	29	theme	infection	1611:1619	arg1	predictors					1567:1576	good predictors	1562:1576	good predictors of B. hyodysenteriae strain 8dII infection in pigs	1562:1627	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	29	theme	infection	1611:1619	arg1	structures					1524:1533	sialic acid-containing structures	1501:1533	sialic acid-containing structures in the mucin O-glycome	1501:1556	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	4	30	theme	broad	697:701	arg1	set					703:705	a broad set	695:705	a broad set of B. hyodysenteriae strains and isolates	695:747	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	30	theme	broad	697:701	arg1	isolates					740:747	isolates	740:747	isolates	740:747	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	30	theme	broad	697:701	arg1	strains					728:734	B. hyodysenteriae strains	710:734	B. hyodysenteriae strains	710:734	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	0	31	theme	Brachyspira	0:10	arg1	Avidity					20:26	Brachyspira Species Avidity	0:26	Brachyspira Species Avidity to Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection	0:108	Brachyspira Species Avidity to Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection Is Species Specific and Varies between Strains.					
34543117	7	32	theme	strain	1599:1604	arg1	infection					1611:1619	B. hyodysenteriae strain 8dII infection	1581:1619	B. hyodysenteriae strain 8dII infection in pigs	1581:1627	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	33	theme	relative	1442:1449	arg1	abundances					1451:1460	the relative abundances	1438:1460	the relative abundances of sialic acid-containing glycans	1438:1494	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	34	theme	B.	1581:1582	arg1	infection					1611:1619	B. hyodysenteriae strain 8dII infection	1581:1619	B. hyodysenteriae strain 8dII infection in pigs	1581:1627	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	2	35	theme	sites	470:474	arg1	number					434:439	the number	430:439	the number of B. hyodysenteriae binding sites in the mucus	430:487	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	4	36	theme	genus	770:774	arg1	level					776:780	a genus level	768:780	a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains)	768:864	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	0	37	theme	Colonic	31:37	arg1	Mucins					39:44	Colonic Mucins	31:44	Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection	31:108	Brachyspira Species Avidity to Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection Is Species Specific and Varies between Strains.					
34543117	6	38	from	changes	1211:1217	arg1	colon					1226:1230	the colon	1222:1230	the colon	1222:1230	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	5	39	theme	acid	1110:1113	arg1	epitopes					1115:1122	sialic acid epitopes	1103:1122	sialic acid epitopes on mucins	1103:1132	Our results show that binding to mucins appears to be specific to B. hyodysenteriae, and within this species, the binding ability to mucins varies between strains/isolates, increases for mucins from pigs with SD, and is associated with sialic acid epitopes on mucins.					
34543117	7	40	from	structures	1524:1533	arg1	O-glycome					1548:1556	the mucin O-glycome	1538:1556	the mucin O-glycome	1538:1556	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	2	41	theme	mucus	358:362	arg1	niche					364:368	the colonic mucus niche	346:368	the colonic mucus niche	346:368	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	7	42	theme	discriminant	1396:1407	arg1	analysis					1409:1416	discriminant analysis	1396:1416	discriminant analysis	1396:1416	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	6	43	theme	acid-containing	1261:1275	arg1	structures					1277:1286	sialic acid-containing structures	1254:1286	sialic acid-containing structures	1254:1286	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	7	44	theme	latent	1378:1383	arg1	structures					1385:1394	latent structures	1378:1394	latent structures	1378:1394	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	6	45	theme	strain	1168:1173	arg1	8dII					1175:1178	B. hyodysenteriae strain 8dII	1150:1178	B. hyodysenteriae strain 8dII	1150:1178	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	5	46	with	pigs	1066:1069	arg1	SD					1076:1077	SD	1076:1077	SD	1076:1077	Our results show that binding to mucins appears to be specific to B. hyodysenteriae, and within this species, the binding ability to mucins varies between strains/isolates, increases for mucins from pigs with SD, and is associated with sialic acid epitopes on mucins.					
34543117	3	47	theme	colon	556:560	arg1	mucins					562:567	colon mucins	556:567	colon mucins	556:567	We previously determined that a B. hyodysenteriae strain binds to colon mucins in a manner that differs between pigs and mucin types.					
34543117	7	48	theme	projections	1363:1373	arg1	models					1428:1433	hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models	1318:1433	hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans	1318:1494	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	49	theme	analysis	1339:1346	arg1	models					1428:1433	hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models	1318:1433	hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans	1318:1494	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	6	50	theme	B.	1150:1151	arg1	8dII					1175:1178	B. hyodysenteriae strain 8dII	1150:1178	B. hyodysenteriae strain 8dII	1150:1178	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	7	51	theme	hierarchical	1318:1329	arg1	OPLS-DA					1419:1425	OPLS-DA	1419:1425	OPLS-DA	1419:1425	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	51	theme	hierarchical	1318:1329	arg1	analysis					1339:1346	hierarchical cluster analysis	1318:1346	hierarchical cluster analysis	1318:1346	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	1	52	from	impact	266:271	arg1	industry					286:293	the swine industry	276:293	the swine industry	276:293	Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry.					
34543117	2	53	theme	hyodysenteriae	299:312	arg1	infection					314:322	B. hyodysenteriae infection	296:322	B. hyodysenteriae infection	296:322	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	1	54	theme	swine	213:217	arg1	dysentery					219:227	swine dysentery	213:227	swine dysentery (SD)	213:232	Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry.					
34543117	1	54	theme	swine	213:217	arg1	disease					237:243	a disease	235:243	a disease that has an economic impact on the swine industry	235:293	Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry.					
34543117	1	54	theme	swine	213:217	arg1	SD					230:231	SD	230:231	SD	230:231	Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry.					
34543117	4	55	dep	B.	710:711	arg1	hyodysenteriae					713:726	hyodysenteriae	713:726	hyodysenteriae	713:726	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	8	56	theme	sialic	1664:1669	arg1	acids					1671:1675	sialic acids	1664:1675	sialic acids	1664:1675	The results emphasize the role of sialic acids in governing B. hyodysenteriae interactions with its host, which may open perspectives for therapeutic strategies.					
34543117	7	57	theme	mucin	1542:1546	arg1	O-glycome					1548:1556	the mucin O-glycome	1538:1556	the mucin O-glycome	1538:1556	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	5	58	theme	binding	981:987	arg1	ability					989:995	the binding ability	977:995	the binding ability to mucins	977:1005	Our results show that binding to mucins appears to be specific to B. hyodysenteriae, and within this species, the binding ability to mucins varies between strains/isolates, increases for mucins from pigs with SD, and is associated with sialic acid epitopes on mucins.					
34543117	6	59	theme	mucin	1191:1195	arg1	shift					1245:1249	a shift	1243:1249	a shift in sialic acid-containing structures	1243:1286	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	6	59	theme	mucin	1191:1195	arg1	changes					1211:1217	mucin glycosylation changes	1191:1217	mucin glycosylation changes	1191:1217	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	8	60	theme	B.	1690:1691	arg1	interactions					1708:1719	B. hyodysenteriae interactions	1690:1719	B. hyodysenteriae interactions	1690:1719	The results emphasize the role of sialic acids in governing B. hyodysenteriae interactions with its host, which may open perspectives for therapeutic strategies.					
34543117	7	61	theme	acid-containing	1508:1522	arg1	predictors					1567:1576	good predictors	1562:1576	good predictors of B. hyodysenteriae strain 8dII infection in pigs	1562:1627	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	61	theme	acid-containing	1508:1522	arg1	structures					1524:1533	sialic acid-containing structures	1501:1533	sialic acid-containing structures in the mucin O-glycome	1501:1556	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	7	62	dep	B.	1581:1582	arg1	hyodysenteriae					1584:1597	hyodysenteriae	1584:1597	hyodysenteriae	1584:1597	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	1	63	contain	has	250:252	arg2	impact					266:271	an economic impact	254:271	an economic impact on the swine industry	254:293	Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry.					
34543117	1	63	contain	has	250:252	arg1	disease					237:243	a disease	235:243	a disease that has an economic impact on the swine industry	235:293	Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry.					
34543117	1	63	contain	has	250:252	arg1	dysentery					219:227	swine dysentery	213:227	swine dysentery (SD)	213:232	Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry.					
34543117	0	64	theme	Brachyspira	73:83	arg1	Infection					100:108	Brachyspira hyodysenteriae Infection	73:108	Brachyspira hyodysenteriae Infection	73:108	Brachyspira Species Avidity to Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection Is Species Specific and Varies between Strains.					
34543117	1	65	theme	Brachyspira	158:168	arg1	hyodysenteriae					170:183	Brachyspira hyodysenteriae	158:183	Brachyspira hyodysenteriae	158:183	Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry.					
34543117	7	66	theme	acid-containing	1472:1486	arg1	glycans					1488:1494	sialic acid-containing glycans	1465:1494	sialic acid-containing glycans	1465:1494	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	4	67	located	observed	679:686	arg2	adhesion					649:656	adhesion	649:656	adhesion to mucins	649:666	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	67	located	observed	679:686	arg2	trait					673:677	a trait	671:677	a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains)	671:864	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	67	located	observed	679:686	arg1	level					776:780	a genus level	768:780	a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains)	768:864	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	0	68	from	Pigs	51:54	arg1	Mucins					39:44	Colonic Mucins	31:44	Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection	31:108	Brachyspira Species Avidity to Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection Is Species Specific and Varies between Strains.					
34543117	4	69	theme	B.	710:711	arg1	strains					728:734	B. hyodysenteriae strains	710:734	B. hyodysenteriae strains	710:734	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	2	70	from	sites	470:474	arg1	mucus					483:487	the mucus	479:487	the mucus	479:487	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	4	71	theme	strains	728:734	arg1	set					703:705	a broad set	695:705	a broad set of B. hyodysenteriae strains and isolates	695:747	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	71	theme	strains	728:734	arg1	isolates					740:747	isolates	740:747	isolates	740:747	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	71	theme	strains	728:734	arg1	strains					728:734	B. hyodysenteriae strains	710:734	B. hyodysenteriae strains	710:734	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	2	72	theme	massive	375:381	arg1	induction					389:397	massive mucus induction	375:397	massive mucus induction	375:397	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	0	73	theme	Species	12:18	arg1	Avidity					20:26	Brachyspira Species Avidity	0:26	Brachyspira Species Avidity to Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection	0:108	Brachyspira Species Avidity to Colonic Mucins from Pigs with and without Brachyspira hyodysenteriae Infection Is Species Specific and Varies between Strains.					
34543117	6	74	from	shift	1245:1249	arg1	structures					1277:1286	sialic acid-containing structures	1254:1286	sialic acid-containing structures	1254:1286	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
34543117	7	75	from	predictors	1567:1576	arg1	pigs					1624:1627	pigs	1624:1627	pigs	1624:1627	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	4	76	dep	innocens	786:793	arg1	strains					857:863	strains	857:863	strains	857:863	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	8	77	theme	therapeutic	1768:1778	arg1	strategies					1780:1789	therapeutic strategies	1768:1789	therapeutic strategies	1768:1789	The results emphasize the role of sialic acids in governing B. hyodysenteriae interactions with its host, which may open perspectives for therapeutic strategies.					
34543117	7	78	theme	8dII	1606:1609	arg1	infection					1611:1619	B. hyodysenteriae strain 8dII infection	1581:1619	B. hyodysenteriae strain 8dII infection in pigs	1581:1627	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	1	79	theme	swine	280:284	arg1	industry					286:293	the swine industry	276:293	the swine industry	276:293	Brachyspira hyodysenteriae is commonly associated with swine dysentery (SD), a disease that has an economic impact on the swine industry.					
34543117	7	80	theme	abundances	1451:1460	arg1	models					1428:1433	hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models	1318:1433	hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans	1318:1494	Thus, we demonstrate through hierarchical cluster analysis and orthogonal projections to latent structures discriminant analysis (OPLS-DA) models of the relative abundances of sialic acid-containing glycans that sialic acid-containing structures in the mucin O-glycome are good predictors of B. hyodysenteriae strain 8dII infection in pigs.					
34543117	2	81	theme	binding	462:468	arg1	sites					470:474	B. hyodysenteriae binding sites	444:474	B. hyodysenteriae binding sites in the mucus	444:487	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	3	82	theme	mucin	611:615	arg1	types					617:621	mucin types	611:621	mucin types	611:621	We previously determined that a B. hyodysenteriae strain binds to colon mucins in a manner that differs between pigs and mucin types.					
34543117	2	83	theme	B.	444:445	arg1	sites					470:474	B. hyodysenteriae binding sites	444:474	B. hyodysenteriae binding sites in the mucus	444:487	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	4	84	theme	isolates	740:747	arg1	set					703:705	a broad set	695:705	a broad set of B. hyodysenteriae strains and isolates	695:747	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	84	theme	isolates	740:747	arg1	isolates					740:747	isolates	740:747	isolates	740:747	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	4	84	theme	isolates	740:747	arg1	strains					728:734	B. hyodysenteriae strains	710:734	B. hyodysenteriae strains	710:734	Here, we investigated if adhesion to mucins is a trait observed across a broad set of B. hyodysenteriae strains and isolates and furthermore at a genus level (B. innocens, B. pilosicoli, B. murdochii, B. hampsonii, and B. intermedia strains).					
34543117	2	85	theme	colonic	350:356	arg1	niche					364:368	the colonic mucus niche	346:368	the colonic mucus niche	346:368	B. hyodysenteriae infection results in changes to the colonic mucus niche with massive mucus induction, which substantially increases the number of B. hyodysenteriae binding sites in the mucus.					
34543117	6	86	with	Infection	1135:1143	arg1	8dII					1175:1178	B. hyodysenteriae strain 8dII	1150:1178	B. hyodysenteriae strain 8dII	1150:1178	Infection with B. hyodysenteriae strain 8dII results in mucin glycosylation changes in the colon, including a shift in sialic acid-containing structures.					
32049016	5	0	theme	O-linked	669:676	arg1	glycans					678:684	these O-linked glycans	663:684	these O-linked glycans	663:684	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	2	1	from	carbohydrate	366:377	arg1	Env					382:384	Env	382:384	Env	382:384	Until now, the dogma has been that HIV-1 lacks O-linked carbohydrate on Env.					
32049016	5	2	theme	neutralization	732:745	arg1	IC50					760:763	IC50	760:763	IC50	760:763	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	5	2	theme	neutralization	732:745	arg1	sensitivity					747:757	neutralization sensitivity	732:757	neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies	732:809	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	3	3	theme	HIV-1	433:437	arg1	isolates					439:446	patient-derived HIV-1 isolates	417:446	patient-derived HIV-1 isolates	417:446	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	1	4	theme	gp120	218:222	arg1	mass					161:164	the mass	157:164	the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1)	157:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	6	5	theme	neutralizing	963:974	arg1	antibodies					976:985	broadly neutralizing antibodies	955:985	broadly neutralizing antibodies	955:985	These findings uncover a structural modification to the HIV-1 Env and suggest a functional role in promoting viral escape from one category of broadly neutralizing antibodies.					
32049016	1	6	theme	N-linked	287:294	arg1	carbohydrate					296:307	N-linked carbohydrate	287:307	N-linked carbohydrate	287:307	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	3	7	theme	isolates	439:446	arg1	subset					407:412	a subset	405:412	a subset of patient-derived HIV-1 isolates	405:446	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	0	8	from	Discovery	0:8	arg1	Shielding					69:77	Shielding	69:77	Shielding	69:77	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	0	8	from	Discovery	0:8	arg1	Envelope					44:51	HIV-1 Envelope	38:51	HIV-1 Envelope	38:51	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	1	9	gly	glycoprotein	188:199	arg1	glycoprotein					188:199	the Envelope (Env) glycoprotein surface subunit (gp120)	169:223	the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1)	169:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	5	10	link	O-linked	669:676	arg1	glycans					678:684	these O-linked glycans	663:684	these O-linked glycans	663:684	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	5	11	from	decrease	720:727	arg1	IC50					760:763	IC50	760:763	IC50	760:763	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	5	11	from	decrease	720:727	arg1	sensitivity					747:757	neutralization sensitivity	732:757	neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies	732:809	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	6	12	theme	functional	892:901	arg1	role					903:906	a functional role	890:906	a functional role	890:906	These findings uncover a structural modification to the HIV-1 Env and suggest a functional role in promoting viral escape from one category of broadly neutralizing antibodies.					
32049016	3	13	theme	variable	485:492	arg1	domain					501:506	the variable 1 (V1) domain	481:506	the variable 1 (V1) domain of Env gp120	481:519	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	3	13	theme	variable	485:492	arg1	gp120					515:519	Env gp120	511:519	Env gp120	511:519	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	1	14	theme	human	228:232	arg1	type					257:260	human immunodeficiency virus type 1	228:262	human immunodeficiency virus type 1 (HIV-1)	228:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	1	14	theme	human	228:232	arg1	HIV-1					265:269	HIV-1	265:269	HIV-1	265:269	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	5	15	theme	more	699:702	arg1	decrease					720:727	a more than 1,000-fold decrease	697:727	a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies	697:809	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	1	16	theme	mass	161:164	arg1	%					152:152	Approximately 50%	136:152	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1)	136:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	1	16	theme	mass	161:164	arg1	mass					161:164	the mass	157:164	the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1)	157:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	5	17	theme	than	704:707	arg1	decrease					720:727	a more than 1,000-fold decrease	697:727	a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies	697:809	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	6	18	from	category	943:950	arg1	escape					927:932	viral escape	921:932	viral escape from one category of broadly neutralizing antibodies	921:985	These findings uncover a structural modification to the HIV-1 Env and suggest a functional role in promoting viral escape from one category of broadly neutralizing antibodies.					
32049016	3	19	theme	V1	497:498	arg1	domain					501:506	the variable 1 (V1) domain	481:506	the variable 1 (V1) domain of Env gp120	481:519	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	3	19	theme	V1	497:498	arg1	gp120					515:519	Env gp120	511:519	Env gp120	511:519	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	5	20	theme	V3-glycan	769:777	arg1	antibodies					800:809	V3-glycan broadly neutralizing antibodies	769:809	V3-glycan broadly neutralizing antibodies	769:809	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	5	21	theme	1,000-fold	709:718	arg1	decrease					720:727	a more than 1,000-fold decrease	697:727	a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies	697:809	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	1	22	theme	immunodeficiency	234:249	arg1	type					257:260	human immunodeficiency virus type 1	228:262	human immunodeficiency virus type 1 (HIV-1)	228:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	1	22	theme	immunodeficiency	234:249	arg1	HIV-1					265:269	HIV-1	265:269	HIV-1	265:269	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	1	23	link	N-linked	287:294	arg1	carbohydrate					296:307	N-linked carbohydrate	287:307	N-linked carbohydrate	287:307	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	6	24	theme	antibodies	976:985	arg1	category					943:950	one category	939:950	one category of broadly neutralizing antibodies	939:985	These findings uncover a structural modification to the HIV-1 Env and suggest a functional role in promoting viral escape from one category of broadly neutralizing antibodies.					
32049016	0	25	theme	Carbohydrate	22:33	arg1	Discovery					0:8	Discovery	0:8	Discovery of O-Linked Carbohydrate on HIV-1 Envelope	0:51	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	0	25	theme	Carbohydrate	22:33	arg1	Role					61:64	Its Role	57:64	Its Role in Shielding against One Category of Broadly Neutralizing Antibodies	57:133	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	1	26	theme	virus	251:255	arg1	type					257:260	human immunodeficiency virus type 1	228:262	human immunodeficiency virus type 1 (HIV-1)	228:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	1	26	theme	virus	251:255	arg1	HIV-1					265:269	HIV-1	265:269	HIV-1	265:269	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	0	27	theme	Neutralizing	111:122	arg1	Antibodies					124:133	Broadly Neutralizing Antibodies	103:133	Broadly Neutralizing Antibodies	103:133	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	3	28	contain	contain	448:454	arg2	carbohydrate					465:476	O-linked carbohydrate	456:476	O-linked carbohydrate	456:476	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	3	28	contain	contain	448:454	arg1	domain					501:506	the variable 1 (V1) domain	481:506	the variable 1 (V1) domain of Env gp120	481:519	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	3	28	contain	contain	448:454	arg1	gp120					515:519	Env gp120	511:519	Env gp120	511:519	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	3	28	contain	contain	448:454	arg1	subset					407:412	a subset	405:412	a subset of patient-derived HIV-1 isolates	405:446	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	3	28	contain	contain	448:454	arg2	subset					407:412	a subset	405:412	a subset of patient-derived HIV-1 isolates	405:446	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	5	29	dep	V3-glycan	769:777	arg1	neutralizing					787:798	neutralizing	787:798	neutralizing	787:798	Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies.					
32049016	1	30	theme	Envelope	173:180	arg1	gp120					218:222	the Envelope (Env) glycoprotein surface subunit (gp120)	169:223	the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1)	169:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	0	31	theme	O-Linked	13:20	arg1	Carbohydrate					22:33	O-Linked Carbohydrate	13:33	O-Linked Carbohydrate	13:33	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	1	32	theme	type	257:260	arg1	gp120					218:222	the Envelope (Env) glycoprotein surface subunit (gp120)	169:223	the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1)	169:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	0	33	theme	Broadly	103:109	arg1	Antibodies					124:133	Broadly Neutralizing Antibodies	103:133	Broadly Neutralizing Antibodies	103:133	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	0	34	theme	HIV-1	38:42	arg1	Envelope					44:51	HIV-1 Envelope	38:51	HIV-1 Envelope	38:51	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	4	35	theme	O-glycosylation	558:572	arg1	presence					541:548	the presence	537:548	the presence of this O-glycosylation both on virions and on gp120 expressed as a secreted protein	537:633	We demonstrate the presence of this O-glycosylation both on virions and on gp120 expressed as a secreted protein.					
32049016	3	36	theme	Env	511:513	arg1	gp120					515:519	Env gp120	511:519	Env gp120	511:519	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	4	37	from	presence	541:548	arg1	virions					582:588	virions	582:588	virions	582:588	We demonstrate the presence of this O-glycosylation both on virions and on gp120 expressed as a secreted protein.					
32049016	4	37	from	presence	541:548	arg1	gp120					597:601	gp120	597:601	gp120 expressed as a secreted protein	597:633	We demonstrate the presence of this O-glycosylation both on virions and on gp120 expressed as a secreted protein.					
32049016	1	38	theme	Env	183:185	arg1	gp120					218:222	the Envelope (Env) glycoprotein surface subunit (gp120)	169:223	the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1)	169:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	6	39	theme	HIV-1	868:872	arg1	Env					874:876	the HIV-1 Env	864:876	the HIV-1 Env	864:876	These findings uncover a structural modification to the HIV-1 Env and suggest a functional role in promoting viral escape from one category of broadly neutralizing antibodies.					
32049016	0	40	theme	Antibodies	124:133	arg1	Category					91:98	One Category	87:98	One Category of Broadly Neutralizing Antibodies	87:133	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	0	41	from	Role	61:64	arg1	Shielding					69:77	Shielding	69:77	Shielding	69:77	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	0	41	from	Role	61:64	arg1	Envelope					44:51	HIV-1 Envelope	38:51	HIV-1 Envelope	38:51	Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies.					
32049016	3	42	theme	gp120	515:519	arg1	domain					501:506	the variable 1 (V1) domain	481:506	the variable 1 (V1) domain of Env gp120	481:519	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	3	42	theme	gp120	515:519	arg1	gp120					515:519	Env gp120	511:519	Env gp120	511:519	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	4	43	theme	secreted	618:625	arg1	protein					627:633	a secreted protein	616:633	a secreted protein	616:633	We demonstrate the presence of this O-glycosylation both on virions and on gp120 expressed as a secreted protein.					
32049016	3	44	link	O-linked	456:463	arg1	carbohydrate					465:476	O-linked carbohydrate	456:476	O-linked carbohydrate	456:476	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	4	45	attach	presence	541:548	arg1	virions					582:588	virions	582:588	virions	582:588	We demonstrate the presence of this O-glycosylation both on virions and on gp120 expressed as a secreted protein.					
32049016	4	45	attach	presence	541:548	arg2	O-glycosylation					558:572	this O-glycosylation	553:572	this O-glycosylation	553:572	We demonstrate the presence of this O-glycosylation both on virions and on gp120 expressed as a secreted protein.					
32049016	4	45	attach	presence	541:548	arg1	gp120					597:601	gp120	597:601	gp120 expressed as a secreted protein	597:633	We demonstrate the presence of this O-glycosylation both on virions and on gp120 expressed as a secreted protein.					
32049016	3	46	theme	patient-derived	417:431	arg1	isolates					439:446	patient-derived HIV-1 isolates	417:446	patient-derived HIV-1 isolates	417:446	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	1	47	theme	glycoprotein	188:199	arg1	gp120					218:222	the Envelope (Env) glycoprotein surface subunit (gp120)	169:223	the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1)	169:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	3	48	link	patient-derived	417:431	arg1	isolates					439:446	patient-derived HIV-1 isolates	417:446	patient-derived HIV-1 isolates	417:446	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	2	49	link	O-linked	357:364	arg1	carbohydrate					366:377	O-linked carbohydrate	357:377	O-linked carbohydrate on Env	357:384	Until now, the dogma has been that HIV-1 lacks O-linked carbohydrate on Env.					
32049016	1	50	theme	surface	201:207	arg1	gp120					218:222	the Envelope (Env) glycoprotein surface subunit (gp120)	169:223	the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1)	169:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	2	51	theme	O-linked	357:364	arg1	carbohydrate					366:377	O-linked carbohydrate	357:377	O-linked carbohydrate on Env	357:384	Until now, the dogma has been that HIV-1 lacks O-linked carbohydrate on Env.					
32049016	1	52	theme	subunit	209:215	arg1	gp120					218:222	the Envelope (Env) glycoprotein surface subunit (gp120)	169:223	the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1)	169:270	Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate.					
32049016	6	53	theme	structural	837:846	arg1	modification					848:859	a structural modification	835:859	a structural modification	835:859	These findings uncover a structural modification to the HIV-1 Env and suggest a functional role in promoting viral escape from one category of broadly neutralizing antibodies.					
32049016	3	54	theme	O-linked	456:463	arg1	carbohydrate					465:476	O-linked carbohydrate	456:476	O-linked carbohydrate	456:476	Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120.					
32049016	6	55	theme	viral	921:925	arg1	escape					927:932	viral escape	921:932	viral escape from one category of broadly neutralizing antibodies	921:985	These findings uncover a structural modification to the HIV-1 Env and suggest a functional role in promoting viral escape from one category of broadly neutralizing antibodies.					
32427862	8	0	theme	Z.	1189:1190	arg1	marina					1192:1197	Z. marina	1189:1197	Z. marina	1189:1197	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	8	1	theme	plant	1162:1166	arg1	AGPs					1168:1171	land plant AGPs	1157:1171	land plant AGPs	1157:1171	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	1	2	theme	monocotyledonous	171:186	arg1	plants					193:198	monocotyledonous land plants	171:198	monocotyledonous land plants that returned to the marine habitat	171:234	Seagrasses evolved from monocotyledonous land plants that returned to the marine habitat.					
32427862	7	3	theme	4-OMe	1093:1097	arg1	acid					1087:1090	4-O-methyl-glucuronic acid	1065:1090	4-O-methyl-glucuronic acid (4-OMe GlcA) residues	1065:1112	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	7	3	theme	4-OMe	1093:1097	arg1	GlcA					1099:1102	4-OMe GlcA	1093:1102	4-OMe GlcA	1093:1102	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	4	4	dep	Zostera	559:565	arg1	L.					574:575	Zostera marina L.	559:575	Zostera marina L.	559:575	AGPs of Zostera marina L. were isolated and structurally characterised by analytical and bioinformatics methods as well as by ELISA with different anti-AGP antibodies.					
32427862	10	5	theme	surface	1461:1467	arg1	role					1436:1439	The role	1432:1439	The role of this polyanionic surface	1432:1467	The role of this polyanionic surface is discussed with regard to adaption to the marine environment.					
32427862	1	6	theme	land	188:191	arg1	plants					193:198	monocotyledonous land plants	171:198	monocotyledonous land plants that returned to the marine habitat	171:234	Seagrasses evolved from monocotyledonous land plants that returned to the marine habitat.					
32427862	0	7	theme	adaption	104:111	arg1	processes					113:121	adaption processes	104:121	adaption processes	104:121	Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments.					
32427862	3	8	attach	present	469:475	arg2	AGPs					411:414	AGPs	411:414	AGPs	411:414	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	8	attach	present	469:475	arg2	arabinogalactan-proteins					385:408	arabinogalactan-proteins	385:408	arabinogalactan-proteins (AGPs)	385:415	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	8	attach	present	469:475	arg1	walls					494:498	seagrass cell walls	480:498	seagrass cell walls	480:498	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	8	attach	present	469:475	arg2	molecules					439:447	important signalling molecules	418:447	important signalling molecules of land plants	418:462	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	6	9	theme	proteome	862:869	arg1	searches					836:843	Bioinformatic searches	822:843	Bioinformatic searches of the Z. marina proteome	822:869	Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs.					
32427862	8	10	with	distinct	1229:1236	arg1	amounts					1248:1254	high amounts	1243:1254	high amounts of uronic acids	1243:1270	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	8	10	with	distinct	1229:1236	arg1	acids					1266:1270	uronic acids	1259:1270	uronic acids	1259:1270	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	7	11	theme	4-O-methyl-glucuronic	1065:1085	arg1	acid					1087:1090	4-O-methyl-glucuronic acid	1065:1090	4-O-methyl-glucuronic acid (4-OMe GlcA) residues	1065:1112	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	7	11	theme	4-O-methyl-glucuronic	1065:1085	arg1	GlcA					1099:1102	4-OMe GlcA	1093:1102	4-OMe GlcA	1093:1102	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	6	12	theme	marina	855:860	arg1	proteome					862:869	the Z. marina proteome	848:869	the Z. marina proteome	848:869	Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs.					
32427862	3	13	dep	evolutionary	506:517	arg1	interest					541:548	interest	541:548	interest	541:548	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	4	14	theme	analytical	625:634	arg1	methods					655:661	analytical and bioinformatics methods	625:661	methods	655:661	AGPs of Zostera marina L. were isolated and structurally characterised by analytical and bioinformatics methods as well as by ELISA with different anti-AGP antibodies.					
32427862	8	15	theme	terminating	1204:1214	arg1	distinct					1229:1236	distinct	1229:1236	distinct	1229:1236	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	8	15	theme	terminating	1204:1214	arg1	residues					1216:1223	the terminating residues	1200:1223	the terminating residues	1200:1223	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	0	16	contain	contain	46:52	arg2	structures					68:77	unique glycan structures	54:77	unique glycan structures	54:77	Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments.					
32427862	0	16	contain	contain	46:52	arg1	Arabinogalactan-proteins					0:23	Arabinogalactan-proteins	0:23	Arabinogalactan-proteins of Zostera marina L.	0:44	Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments.					
32427862	2	17	theme	wall	301:304	arg1	composition					306:316	cell wall composition	296:316	cell wall composition	296:316	This transition was accomplished by substantial changes in cell wall composition, revealing habitat-driven adaption to the new environment.					
32427862	4	18	theme	anti-AGP	698:705	arg1	antibodies					707:716	different anti-AGP antibodies	688:716	different anti-AGP antibodies	688:716	AGPs of Zostera marina L. were isolated and structurally characterised by analytical and bioinformatics methods as well as by ELISA with different anti-AGP antibodies.					
32427862	6	19	theme	AGPs	930:933	arg1	AGPs					894:897	9 classical AGPs	882:897	9 classical AGPs	882:897	Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs.					
32427862	6	19	theme	AGPs	930:933	arg1	number					911:916	a large number	903:916	a large number of chimeric AGPs	903:933	Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs.					
32427862	2	20	theme	cell	296:299	arg1	composition					306:316	cell wall composition	296:316	cell wall composition	296:316	This transition was accomplished by substantial changes in cell wall composition, revealing habitat-driven adaption to the new environment.					
32427862	4	21	theme	different	688:696	arg1	antibodies					707:716	different anti-AGP antibodies	688:716	different anti-AGP antibodies	688:716	AGPs of Zostera marina L. were isolated and structurally characterised by analytical and bioinformatics methods as well as by ELISA with different anti-AGP antibodies.					
32427862	3	22	theme	important	418:426	arg1	arabinogalactan-proteins					385:408	arabinogalactan-proteins	385:408	arabinogalactan-proteins (AGPs)	385:415	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	22	theme	important	418:426	arg1	molecules					439:447	important signalling molecules	418:447	important signalling molecules of land plants	418:462	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	6	23	theme	chimeric	921:928	arg1	AGPs					930:933	chimeric AGPs	921:933	chimeric AGPs	921:933	Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs.					
32427862	3	24	located	present	469:475	arg2	AGPs					411:414	AGPs	411:414	AGPs	411:414	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	24	located	present	469:475	arg2	arabinogalactan-proteins					385:408	arabinogalactan-proteins	385:408	arabinogalactan-proteins (AGPs)	385:415	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	24	located	present	469:475	arg1	walls					494:498	seagrass cell walls	480:498	seagrass cell walls	480:498	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	24	located	present	469:475	arg2	molecules					439:447	important signalling molecules	418:447	important signalling molecules of land plants	418:462	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	25	theme	signalling	428:437	arg1	arabinogalactan-proteins					385:408	arabinogalactan-proteins	385:408	arabinogalactan-proteins (AGPs)	385:415	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	25	theme	signalling	428:437	arg1	molecules					439:447	important signalling molecules	418:447	important signalling molecules of land plants	418:462	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	10	26	theme	marine	1513:1518	arg1	environment					1520:1530	the marine environment	1509:1530	the marine environment	1509:1530	The role of this polyanionic surface is discussed with regard to adaption to the marine environment.					
32427862	8	27	theme	high	1243:1246	arg1	amounts					1248:1254	high amounts	1243:1254	high amounts of uronic acids	1243:1270	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	8	27	theme	high	1243:1246	arg1	acids					1266:1270	uronic acids	1259:1270	uronic acids	1259:1270	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	7	28	theme	high	995:998	arg1	degree					1000:1005	a high degree	993:1005	a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues	993:1112	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	8	29	theme	common	1128:1133	arg1	structure					1144:1152	the common backbone structure	1124:1152	the common backbone structure of land plant AGPs	1124:1171	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	6	30	theme	Z.	852:853	arg1	proteome					862:869	the Z. marina proteome	848:869	the Z. marina proteome	848:869	Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs.					
32427862	2	31	theme	habitat-driven	329:342	arg1	adaption					344:351	habitat-driven adaption	329:351	habitat-driven adaption to the new environment	329:374	This transition was accomplished by substantial changes in cell wall composition, revealing habitat-driven adaption to the new environment.					
32427862	0	32	theme	Zostera	28:34	arg1	Arabinogalactan-proteins					0:23	Arabinogalactan-proteins	0:23	Arabinogalactan-proteins of Zostera marina L.	0:44	Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments.					
32427862	0	33	theme	saline	126:131	arg1	environments					133:144	saline environments	126:144	saline environments	126:144	Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments.					
32427862	9	34	dep	enzymes	1328:1334	arg1	methyltransferases					1362:1379	methyltransferases	1362:1379	methyltransferases	1362:1379	These differences likely result from the glycan-active enzymes (glycosyltransferases and methyltransferases) and are essential for calcium-binding properties.					
32427862	9	34	dep	enzymes	1328:1334	arg1	glycosyltransferases					1337:1356	glycosyltransferases	1337:1356	glycosyltransferases	1337:1356	These differences likely result from the glycan-active enzymes (glycosyltransferases and methyltransferases) and are essential for calcium-binding properties.					
32427862	1	35	theme	marine	221:226	arg1	habitat					228:234	the marine habitat	217:234	the marine habitat	217:234	Seagrasses evolved from monocotyledonous land plants that returned to the marine habitat.					
32427862	3	36	theme	land	452:455	arg1	plants					457:462	land plants	452:462	land plants	452:462	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	9	37	theme	calcium-binding	1404:1418	arg1	properties					1420:1429	calcium-binding properties	1404:1429	calcium-binding properties	1404:1429	These differences likely result from the glycan-active enzymes (glycosyltransferases and methyltransferases) and are essential for calcium-binding properties.					
32427862	8	38	theme	land	1157:1160	arg1	AGPs					1168:1171	land plant AGPs	1157:1171	land plant AGPs	1157:1171	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	2	39	from	changes	285:291	arg1	composition					306:316	cell wall composition	296:316	cell wall composition	296:316	This transition was accomplished by substantial changes in cell wall composition, revealing habitat-driven adaption to the new environment.					
32427862	3	40	theme	seagrass	480:487	arg1	walls					494:498	seagrass cell walls	480:498	seagrass cell walls	480:498	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	41	theme	plants	457:462	arg1	arabinogalactan-proteins					385:408	arabinogalactan-proteins	385:408	arabinogalactan-proteins (AGPs)	385:415	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	41	theme	plants	457:462	arg1	molecules					439:447	important signalling molecules	418:447	important signalling molecules of land plants	418:462	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	4	42	theme	Zostera	559:565	arg1	AGPs					551:554	AGPs	551:554	AGPs of Zostera marina L.	551:575	AGPs of Zostera marina L. were isolated and structurally characterised by analytical and bioinformatics methods as well as by ELISA with different anti-AGP antibodies.					
32427862	5	43	theme	isothermal	767:776	arg1	ITC					801:803	ITC	801:803	ITC	801:803	Calcium-binding capacity of AGPs was studied by isothermal titration calorimetry (ITC) and microscopy.					
32427862	5	43	theme	isothermal	767:776	arg1	calorimetry					788:798	isothermal titration calorimetry	767:798	isothermal titration calorimetry (ITC)	767:804	Calcium-binding capacity of AGPs was studied by isothermal titration calorimetry (ITC) and microscopy.					
32427862	6	44	theme	large	905:909	arg1	number					911:916	a large number	903:916	a large number of chimeric AGPs	903:933	Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs.					
32427862	3	45	theme	cell	489:492	arg1	walls					494:498	seagrass cell walls	480:498	seagrass cell walls	480:498	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	0	46	theme	glycan	61:66	arg1	structures					68:77	unique glycan structures	54:77	unique glycan structures	54:77	Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments.					
32427862	5	47	theme	titration	778:786	arg1	ITC					801:803	ITC	801:803	ITC	801:803	Calcium-binding capacity of AGPs was studied by isothermal titration calorimetry (ITC) and microscopy.					
32427862	5	47	theme	titration	778:786	arg1	calorimetry					788:798	isothermal titration calorimetry	767:798	isothermal titration calorimetry (ITC)	767:804	Calcium-binding capacity of AGPs was studied by isothermal titration calorimetry (ITC) and microscopy.					
32427862	5	48	theme	Calcium-binding	719:733	arg1	capacity					735:742	Calcium-binding capacity	719:742	Calcium-binding capacity of AGPs	719:750	Calcium-binding capacity of AGPs was studied by isothermal titration calorimetry (ITC) and microscopy.					
32427862	7	49	theme	content	1042:1048	arg1	degree					1000:1005	a high degree	993:1005	a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues	993:1112	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	0	50	theme	unique	54:59	arg1	structures					68:77	unique glycan structures	54:77	unique glycan structures	54:77	Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments.					
32427862	10	51	theme	polyanionic	1449:1459	arg1	surface					1461:1467	this polyanionic surface	1444:1467	this polyanionic surface	1444:1467	The role of this polyanionic surface is discussed with regard to adaption to the marine environment.					
32427862	3	52	from	walls	494:498	arg1	present					469:475	present	469:475	present	469:475	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	3	53	theme	plant	523:527	arg1	development					529:539	plant development	523:539	plant development	523:539	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	6	54	theme	Bioinformatic	822:834	arg1	searches					836:843	Bioinformatic searches	822:843	Bioinformatic searches of the Z. marina proteome	822:869	Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs.					
32427862	7	55	theme	acid	1087:1090	arg1	residues					1105:1112	4-O-methyl-glucuronic acid (4-OMe GlcA) residues	1065:1112	4-O-methyl-glucuronic acid (4-OMe GlcA) residues	1065:1112	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	4	56	with	ELISA	677:681	arg1	antibodies					707:716	different anti-AGP antibodies	688:716	different anti-AGP antibodies	688:716	AGPs of Zostera marina L. were isolated and structurally characterised by analytical and bioinformatics methods as well as by ELISA with different anti-AGP antibodies.					
32427862	7	57	theme	branching	1010:1018	arg1	degree					1000:1005	a high degree	993:1005	a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues	993:1112	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	2	58	theme	new	360:362	arg1	environment					364:374	the new environment	356:374	the new environment	356:374	This transition was accomplished by substantial changes in cell wall composition, revealing habitat-driven adaption to the new environment.					
32427862	7	59	theme	unique	966:971	arg1	degree					1000:1005	a high degree	993:1005	a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues	993:1112	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	7	59	theme	unique	966:971	arg1	features					973:980	unique features	966:980	unique features	966:980	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	5	60	theme	AGPs	747:750	arg1	capacity					735:742	Calcium-binding capacity	719:742	Calcium-binding capacity of AGPs	719:750	Calcium-binding capacity of AGPs was studied by isothermal titration calorimetry (ITC) and microscopy.					
32427862	6	61	theme	classical	884:892	arg1	AGPs					894:897	9 classical AGPs	882:897	9 classical AGPs	882:897	Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs.					
32427862	0	62	dep	Zostera	28:34	arg1	L.					43:44	Zostera marina L.	28:44	Zostera marina L.	28:44	Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments.					
32427862	8	63	theme	AGPs	1168:1171	arg1	structure					1144:1152	the common backbone structure	1124:1152	the common backbone structure of land plant AGPs	1124:1171	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	7	64	theme	glycan	940:945	arg1	structures					947:956	The glycan structures	936:956	The glycan structures	936:956	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	4	65	theme	bioinformatics	640:653	arg1	methods					655:661	analytical and bioinformatics methods	625:661	methods	655:661	AGPs of Zostera marina L. were isolated and structurally characterised by analytical and bioinformatics methods as well as by ELISA with different anti-AGP antibodies.					
32427862	2	66	theme	substantial	273:283	arg1	changes					285:291	substantial changes	273:291	substantial changes in cell wall composition	273:316	This transition was accomplished by substantial changes in cell wall composition, revealing habitat-driven adaption to the new environment.					
32427862	7	67	theme	high	1037:1040	arg1	content					1042:1048	an unusually high content	1024:1048	an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues	1024:1112	The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues.					
32427862	8	68	theme	acids	1266:1270	arg1	amounts					1248:1254	high amounts	1243:1254	high amounts of uronic acids	1243:1270	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	8	68	theme	acids	1266:1270	arg1	acids					1266:1270	uronic acids	1259:1270	uronic acids	1259:1270	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	8	69	theme	uronic	1259:1264	arg1	acids					1266:1270	uronic acids	1259:1270	uronic acids	1259:1270	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32427862	9	70	theme	glycan-active	1314:1326	arg1	enzymes					1328:1334	the glycan-active enzymes	1310:1334	the glycan-active enzymes (glycosyltransferases and methyltransferases)	1310:1380	These differences likely result from the glycan-active enzymes (glycosyltransferases and methyltransferases) and are essential for calcium-binding properties.					
32427862	3	71	from	present	469:475	arg1	walls					494:498	seagrass cell walls	480:498	seagrass cell walls	480:498	Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest.					
32427862	8	72	theme	backbone	1135:1142	arg1	structure					1144:1152	the common backbone structure	1124:1152	the common backbone structure of land plant AGPs	1124:1171	Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids.					
32057430	1	0	from	allergen	167:174	arg1	powder					184:189	milk powder	179:189	milk powder	179:189	Bovine β-lactoglobulin (β-LG) is the major allergen in milk powder.					
32057430	2	1	from	presence	286:293	arg1	120					322:324	120	322:324	120	322:324	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	1	from	presence	286:293	arg1	180°C					330:334	180°C	330:334	180°C	330:334	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	6	2	theme	high	979:982	arg1	180°C					997:1001	180°C	997:1001	180°C	997:1001	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	6	2	theme	high	979:982	arg1	temperature					984:994	high temperature	979:994	high temperature (180°C) spray drying	979:1015	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	0	3	from	reduction	4:12	arg1	capacity					67:74	the immunoglobulin G and immunoglobulin E binding capacity	17:74	the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin	17:93	The reduction in the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin via spray-drying technology.					
32057430	6	4	from	reduction	930:938	arg1	capacity					963:970	the IgG/IgE binding capacity	943:970	the IgG/IgE binding capacity during high temperature (180°C) spray drying	943:1015	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	3	5	located	found	434:438	arg2	change					423:428	no change	420:428	no change	420:428	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	3	5	located	found	434:438	arg1	temperature					398:408	a drying temperature	389:408	a drying temperature of 120°C	389:417	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	3	5	located	found	434:438	arg1	capacity					463:470	the IgG/IgE binding capacity	443:470	the IgG/IgE binding capacity of β-LG	443:478	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	4	6	theme	β-LG	634:637	arg1	aggregation					619:629	aggregation	619:629	aggregation of β-LG	619:637	At a drying temperature of 180°C, aggregation of β-LG occurred, leading to a decrease in the IgG/IgE binding capacity.					
32057430	2	7	from	absence	298:304	arg1	120					322:324	120	322:324	120	322:324	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	7	from	absence	298:304	arg1	180°C					330:334	180°C	330:334	180°C	330:334	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	0	8	theme	β-lactoglobulin	79:93	arg1	capacity					67:74	the immunoglobulin G and immunoglobulin E binding capacity	17:74	the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin	17:93	The reduction in the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin via spray-drying technology.					
32057430	4	9	theme	binding	686:692	arg1	capacity					694:701	the IgG/IgE binding capacity	674:701	the IgG/IgE binding capacity	674:701	At a drying temperature of 180°C, aggregation of β-LG occurred, leading to a decrease in the IgG/IgE binding capacity.					
32057430	5	10	from	side-chains	746:756	arg1	β-LG					761:764	β-LG	761:764	β-LG	761:764	When α-lactose was also present, 7 lysine side-chains in β-LG were modified by glycation and the IgG/IgE binding capacity was further decreased.					
32057430	2	11	from	120	322:324	arg1	α-lactose					309:317	α-lactose	309:317	α-lactose at 120 and 180°C	309:334	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	11	from	120	322:324	arg1	absence					298:304	absence	298:304	absence	298:304	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	11	from	120	322:324	arg1	presence					286:293	presence	286:293	presence	286:293	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	1	12	theme	major	161:165	arg1	β-lactoglobulin					131:145	Bovine β-lactoglobulin	124:145	Bovine β-lactoglobulin (β-LG)	124:152	Bovine β-lactoglobulin (β-LG) is the major allergen in milk powder.					
32057430	1	12	theme	major	161:165	arg1	allergen					167:174	the major allergen	157:174	the major allergen in milk powder	157:189	Bovine β-lactoglobulin (β-LG) is the major allergen in milk powder.					
32057430	0	13	theme	spray-drying	99:110	arg1	technology					112:121	spray-drying technology	99:121	spray-drying technology	99:121	The reduction in the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin via spray-drying technology.					
32057430	2	14	theme	spray	266:270	arg1	drying					272:277	spray drying	266:277	spray drying in the presence or absence of α-lactose at 120 and 180°C	266:334	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	6	15	theme	spray	1004:1008	arg1	drying					1010:1015	high temperature (180°C) spray drying	979:1015	high temperature (180°C) spray drying	979:1015	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	2	16	theme	mass	367:370	arg1	spectrometry					372:383	mass spectrometry	367:383	mass spectrometry	367:383	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	6	17	theme	structural	878:887	arg1	changes					889:895	the glycation and structural changes	860:895	changes	889:895	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	6	17	theme	structural	878:887	arg1	responsible					910:920	responsible	910:920	responsible	910:920	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	2	18	from	180°C	330:334	arg1	α-lactose					309:317	α-lactose	309:317	α-lactose at 120 and 180°C	309:334	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	18	from	180°C	330:334	arg1	absence					298:304	absence	298:304	absence	298:304	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	18	from	180°C	330:334	arg1	presence					286:293	presence	286:293	presence	286:293	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	3	19	theme	amino	515:519	arg1	content					527:533	free amino group content	510:533	free amino group content	510:533	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	3	20	theme	free	510:513	arg1	content					527:533	free amino group content	510:533	free amino group content	510:533	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	3	21	theme	group	521:525	arg1	content					527:533	free amino group content	510:533	free amino group content	510:533	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	3	22	located	observed	498:505	arg1	content					527:533	free amino group content	510:533	free amino group content	510:533	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	3	22	located	observed	498:505	arg1	intensity					549:557	fluorescence intensity	536:557	fluorescence intensity	536:557	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	3	22	located	observed	498:505	arg2	change					487:492	no change	484:492	no change	484:492	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	3	22	located	observed	498:505	arg1	glycation					574:582	detectable glycation	563:582	detectable glycation	563:582	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	1	23	theme	milk	179:182	arg1	powder					184:189	milk powder	179:189	milk powder	179:189	Bovine β-lactoglobulin (β-LG) is the major allergen in milk powder.					
32057430	5	24	theme	lysine	739:744	arg1	side-chains					746:756	7 lysine side-chains	737:756	7 lysine side-chains in β-LG	737:764	When α-lactose was also present, 7 lysine side-chains in β-LG were modified by glycation and the IgG/IgE binding capacity was further decreased.					
32057430	6	25	from	changes	889:895	arg1	β-LG					900:903	β-LG	900:903	β-LG	900:903	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	4	26	from	decrease	662:669	arg1	capacity					694:701	the IgG/IgE binding capacity	674:701	the IgG/IgE binding capacity	674:701	At a drying temperature of 180°C, aggregation of β-LG occurred, leading to a decrease in the IgG/IgE binding capacity.					
32057430	4	27	theme	IgG/IgE	678:684	arg1	capacity					694:701	the IgG/IgE binding capacity	674:701	the IgG/IgE binding capacity	674:701	At a drying temperature of 180°C, aggregation of β-LG occurred, leading to a decrease in the IgG/IgE binding capacity.					
32057430	3	28	theme	fluorescence	536:547	arg1	intensity					549:557	fluorescence intensity	536:557	fluorescence intensity	536:557	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	2	29	dep	capacity	212:219	arg1	The					192:194	The	192:194	The	192:194	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	3	30	theme	binding	455:461	arg1	capacity					463:470	the IgG/IgE binding capacity	443:470	the IgG/IgE binding capacity of β-LG	443:478	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	5	31	theme	IgG/IgE	801:807	arg1	capacity					817:824	the IgG/IgE binding capacity	797:824	the IgG/IgE binding capacity	797:824	When α-lactose was also present, 7 lysine side-chains in β-LG were modified by glycation and the IgG/IgE binding capacity was further decreased.					
32057430	0	32	theme	G	36:36	arg1	capacity					67:74	the immunoglobulin G and immunoglobulin E binding capacity	17:74	the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin	17:93	The reduction in the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin via spray-drying technology.					
32057430	2	33	dep	presence	286:293	arg1	the					282:284	the	282:284	the	282:284	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	34	theme	structural	225:234	arg1	characteristics					236:250	structural characteristics	225:250	structural characteristics	225:250	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	4	35	theme	180°C	612:616	arg1	temperature					597:607	a drying temperature	588:607	a drying temperature of 180°C	588:616	At a drying temperature of 180°C, aggregation of β-LG occurred, leading to a decrease in the IgG/IgE binding capacity.					
32057430	4	36	theme	drying	590:595	arg1	temperature					597:607	a drying temperature	588:607	a drying temperature of 180°C	588:616	At a drying temperature of 180°C, aggregation of β-LG occurred, leading to a decrease in the IgG/IgE binding capacity.					
32057430	0	37	theme	immunoglobulin	21:34	arg1	G					36:36	immunoglobulin G	21:36	immunoglobulin G	21:36	The reduction in the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin via spray-drying technology.					
32057430	3	38	theme	drying	391:396	arg1	temperature					398:408	a drying temperature	389:408	a drying temperature of 120°C	389:417	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	6	39	theme	binding	955:961	arg1	capacity					963:970	the IgG/IgE binding capacity	943:970	the IgG/IgE binding capacity during high temperature (180°C) spray drying	943:1015	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	0	40	theme	immunoglobulin	42:55	arg1	E					57:57	immunoglobulin E	42:57	immunoglobulin E	42:57	The reduction in the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin via spray-drying technology.					
32057430	6	41	theme	glycation	864:872	arg1	changes					889:895	the glycation and structural changes	860:895	changes	889:895	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	6	41	theme	glycation	864:872	arg1	responsible					910:920	responsible	910:920	responsible	910:920	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	3	42	theme	120°C	413:417	arg1	temperature					398:408	a drying temperature	389:408	a drying temperature of 120°C	389:417	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	3	43	theme	IgG/IgE	447:453	arg1	capacity					463:470	the IgG/IgE binding capacity	443:470	the IgG/IgE binding capacity of β-LG	443:478	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	6	44	theme	temperature	984:994	arg1	drying					1010:1015	high temperature (180°C) spray drying	979:1015	high temperature (180°C) spray drying	979:1015	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	2	45	theme	IgG/IgE	196:202	arg1	capacity					212:219	IgG/IgE binding capacity	196:219	IgG/IgE binding capacity	196:219	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	46	theme	binding	204:210	arg1	capacity					212:219	IgG/IgE binding capacity	196:219	IgG/IgE binding capacity	196:219	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	1	47	theme	Bovine	124:129	arg1	β-lactoglobulin					131:145	Bovine β-lactoglobulin	124:145	Bovine β-lactoglobulin (β-LG)	124:152	Bovine β-lactoglobulin (β-LG) is the major allergen in milk powder.					
32057430	1	47	theme	Bovine	124:129	arg1	allergen					167:174	the major allergen	157:174	the major allergen in milk powder	157:189	Bovine β-lactoglobulin (β-LG) is the major allergen in milk powder.					
32057430	1	47	theme	Bovine	124:129	arg1	β-LG					148:151	β-LG	148:151	β-LG	148:151	Bovine β-lactoglobulin (β-LG) is the major allergen in milk powder.					
32057430	2	48	from	drying	272:277	arg1	absence					298:304	absence	298:304	absence	298:304	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	48	from	drying	272:277	arg1	presence					286:293	presence	286:293	presence	286:293	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	3	49	theme	detectable	563:572	arg1	glycation					574:582	detectable glycation	563:582	detectable glycation	563:582	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	6	50	theme	IgG/IgE	947:953	arg1	capacity					963:970	the IgG/IgE binding capacity	943:970	the IgG/IgE binding capacity during high temperature (180°C) spray drying	943:1015	Therefore, the glycation and structural changes in β-LG were responsible for the reduction in the IgG/IgE binding capacity during high temperature (180°C) spray drying.					
32057430	2	51	theme	β-LG	255:258	arg1	capacity					212:219	IgG/IgE binding capacity	196:219	IgG/IgE binding capacity	196:219	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	51	theme	β-LG	255:258	arg1	characteristics					236:250	structural characteristics	225:250	structural characteristics	225:250	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	52	theme	α-lactose	309:317	arg1	absence					298:304	absence	298:304	absence	298:304	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	2	52	theme	α-lactose	309:317	arg1	presence					286:293	presence	286:293	presence	286:293	The IgG/IgE binding capacity and structural characteristics of β-LG after spray drying in the presence or absence of α-lactose at 120 and 180°C were investigated by ELISA and mass spectrometry.					
32057430	0	53	theme	binding	59:65	arg1	capacity					67:74	the immunoglobulin G and immunoglobulin E binding capacity	17:74	the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin	17:93	The reduction in the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin via spray-drying technology.					
32057430	5	54	mod	modified	771:778	arg3	glycation					783:791	glycation	783:791	glycation	783:791	When α-lactose was also present, 7 lysine side-chains in β-LG were modified by glycation and the IgG/IgE binding capacity was further decreased.					
32057430	5	54	mod	modified	771:778	arg1	side-chains					746:756	7 lysine side-chains	737:756	7 lysine side-chains in β-LG	737:764	When α-lactose was also present, 7 lysine side-chains in β-LG were modified by glycation and the IgG/IgE binding capacity was further decreased.					
32057430	3	55	theme	β-LG	475:478	arg1	capacity					463:470	the IgG/IgE binding capacity	443:470	the IgG/IgE binding capacity of β-LG	443:478	At a drying temperature of 120°C, no change was found in the IgG/IgE binding capacity of β-LG and no change was observed in free amino group content, fluorescence intensity, or detectable glycation.					
32057430	0	56	theme	E	57:57	arg1	capacity					67:74	the immunoglobulin G and immunoglobulin E binding capacity	17:74	the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin	17:93	The reduction in the immunoglobulin G and immunoglobulin E binding capacity of β-lactoglobulin via spray-drying technology.					
32057430	5	57	theme	binding	809:815	arg1	capacity					817:824	the IgG/IgE binding capacity	797:824	the IgG/IgE binding capacity	797:824	When α-lactose was also present, 7 lysine side-chains in β-LG were modified by glycation and the IgG/IgE binding capacity was further decreased.					
33915325	8	0	theme	promising	1210:1218	arg1	scaffolds					1235:1243	promising immunoglobulin scaffolds	1210:1243	promising immunoglobulin scaffolds	1210:1243	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	0	1	theme	protein	86:92	arg1	stabilization					94:106	protein stabilization	86:106	protein stabilization	86:106	Abolition of aggregation of CH2 domain of human IgG1 when combining glycosylation and protein stabilization.					
33915325	8	2	theme	aggregation	1135:1145	arg1	behavior					1147:1154	the aggregation behavior	1131:1154	the aggregation behavior of a protein	1131:1167	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	8	3	theme	stabilization	1104:1116	arg1	strategy					1189:1196	a fruitful strategy	1178:1196	a fruitful strategy to generate promising immunoglobulin scaffolds	1178:1243	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	8	3	theme	stabilization	1104:1116	arg1	combination					1071:1081	the combination	1067:1081	the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein	1067:1167	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	2	4	theme	scaffold	281:288	arg1	it					258:259	it	258:259	it	258:259	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	2	4	theme	scaffold	281:288	arg1	candidate					290:298	a promising scaffold candidate	269:298	a promising scaffold candidate for developing novel therapeutic approaches	269:342	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	2	5	theme	stable	236:241	arg1	domain					243:248	the least stable domain	226:248	the least stable domain in IgG	226:255	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	2	5	theme	stable	236:241	arg1	domain					216:221	the CH2 domain	208:221	the CH2 domain	208:221	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	8	6	theme	immunoglobulin	1220:1233	arg1	scaffolds					1235:1243	promising immunoglobulin scaffolds	1210:1243	promising immunoglobulin scaffolds	1210:1243	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	8	7	theme	protein	1017:1023	arg1	aggregation					1025:1035	protein aggregation	1017:1035	protein aggregation	1017:1035	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	1	8	theme	human	153:157	arg1	IgG					177:179	IgG	177:179	IgG	177:179	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	1	8	theme	human	153:157	arg1	G					174:174	human Immunoglobulin G	153:174	the human Immunoglobulin G (IgG) constant region	149:196	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	4	9	theme	domain	514:519	arg1	glycosylation					489:501	glycosylation	489:501	glycosylation of the CH2 domain	489:519	Herein, we verified that glycosylation of the CH2 domain decreased both, its tendency to aggregate and its immunogenicity in mice, suggesting that aggregation and immunogenicity are related.					
33915325	1	10	theme	Immunoglobulin	159:172	arg1	IgG					177:179	IgG	177:179	IgG	177:179	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	1	10	theme	Immunoglobulin	159:172	arg1	G					174:174	human Immunoglobulin G	153:174	the human Immunoglobulin G (IgG) constant region	149:196	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	8	11	theme	protein	1161:1167	arg1	behavior					1147:1154	the aggregation behavior	1131:1154	the aggregation behavior of a protein	1131:1167	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	4	12	theme	CH2	510:512	arg1	domain					514:519	the CH2 domain	506:519	the CH2 domain	506:519	Herein, we verified that glycosylation of the CH2 domain decreased both, its tendency to aggregate and its immunogenicity in mice, suggesting that aggregation and immunogenicity are related.					
33915325	2	13	theme	therapeutic	321:331	arg1	approaches					333:342	novel therapeutic approaches	315:342	novel therapeutic approaches	315:342	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	5	14	theme	domain	732:737	arg1	version					713:719	a stabilized version	700:719	P. pastoris a stabilized version of the CH2 domain with and without glycan	688:761	In addition, we have produced in P. pastoris a stabilized version of the CH2 domain with and without glycan, and their propensity to aggregate evaluated.					
33915325	4	15	from	immunogenicity	571:584	arg1	mice					589:592	mice	589:592	mice	589:592	Herein, we verified that glycosylation of the CH2 domain decreased both, its tendency to aggregate and its immunogenicity in mice, suggesting that aggregation and immunogenicity are related.					
33915325	0	16	theme	domain	32:37	arg1	aggregation					13:23	aggregation	13:23	aggregation of CH2 domain of human IgG1	13:51	Abolition of aggregation of CH2 domain of human IgG1 when combining glycosylation and protein stabilization.					
33915325	2	17	theme	promising	271:279	arg1	it					258:259	it	258:259	it	258:259	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	2	17	theme	promising	271:279	arg1	candidate					290:298	a promising scaffold candidate	269:298	a promising scaffold candidate for developing novel therapeutic approaches	269:342	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	1	18	theme	G	174:174	arg1	region					191:196	the human Immunoglobulin G (IgG) constant region	149:196	the human Immunoglobulin G (IgG) constant region	149:196	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	3	19	theme	CH2	415:417	arg1	domain					419:424	CH2 domain	415:424	CH2 domain	415:424	Recently, we succeeded in preparing glycosylated and non-glycosylated CH2 domain in the host organism Pichia pastoris.					
33915325	0	20	theme	CH2	28:30	arg1	domain					32:37	CH2 domain	28:37	CH2 domain of human IgG1	28:51	Abolition of aggregation of CH2 domain of human IgG1 when combining glycosylation and protein stabilization.					
33915325	0	20	theme	CH2	28:30	arg1	IgG1					48:51	human IgG1	42:51	human IgG1	42:51	Abolition of aggregation of CH2 domain of human IgG1 when combining glycosylation and protein stabilization.					
33915325	6	21	theme	domain	894:899	arg1	aggregation					871:881	the aggregation	867:881	the aggregation of the CH2 domain	867:899	We found that stabilization alone significantly decreased the aggregation of the CH2 domain.					
33915325	5	22	theme	CH2	728:730	arg1	domain					732:737	the CH2 domain	724:737	the CH2 domain with and without glycan	724:761	In addition, we have produced in P. pastoris a stabilized version of the CH2 domain with and without glycan, and their propensity to aggregate evaluated.					
33915325	1	23	theme	constant	182:189	arg1	region					191:196	the human Immunoglobulin G (IgG) constant region	149:196	the human Immunoglobulin G (IgG) constant region	149:196	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	0	24	theme	human	42:46	arg1	IgG1					48:51	human IgG1	42:51	human IgG1	42:51	Abolition of aggregation of CH2 domain of human IgG1 when combining glycosylation and protein stabilization.					
33915325	4	25	from	tendency	541:548	arg1	mice					589:592	mice	589:592	mice	589:592	Herein, we verified that glycosylation of the CH2 domain decreased both, its tendency to aggregate and its immunogenicity in mice, suggesting that aggregation and immunogenicity are related.					
33915325	8	26	theme	fruitful	1180:1187	arg1	strategy					1189:1196	a fruitful strategy	1178:1196	a fruitful strategy to generate promising immunoglobulin scaffolds	1178:1243	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	8	26	theme	fruitful	1180:1187	arg1	combination					1071:1081	the combination	1067:1081	the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein	1067:1167	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	5	27	theme	stabilized	702:711	arg1	version					713:719	a stabilized version	700:719	P. pastoris a stabilized version of the CH2 domain with and without glycan	688:761	In addition, we have produced in P. pastoris a stabilized version of the CH2 domain with and without glycan, and their propensity to aggregate evaluated.					
33915325	3	28	theme	Pichia	447:452	arg1	pastoris					454:461	the host organism Pichia pastoris	429:461	the host organism Pichia pastoris	429:461	Recently, we succeeded in preparing glycosylated and non-glycosylated CH2 domain in the host organism Pichia pastoris.					
33915325	5	29	dep	P. pastoris	688:698	arg1	version					713:719	a stabilized version	700:719	P. pastoris a stabilized version of the CH2 domain with and without glycan	688:761	In addition, we have produced in P. pastoris a stabilized version of the CH2 domain with and without glycan, and their propensity to aggregate evaluated.					
33915325	3	30	dep	non-glycosylated	398:413	arg1	domain					419:424	CH2 domain	415:424	CH2 domain	415:424	Recently, we succeeded in preparing glycosylated and non-glycosylated CH2 domain in the host organism Pichia pastoris.					
33915325	7	31	theme	stabilization	949:961	arg1	combination					916:926	the combination	912:926	the combination of glycosylation and stabilization	912:961	Moreover, the combination of glycosylation and stabilization completely suppressed its aggregation behavior.					
33915325	1	32	theme	CH2	113:115	arg1	domain					117:122	The CH2 domain	109:122	The CH2 domain	109:122	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	1	32	theme	CH2	113:115	arg1	element					138:144	a critical element	127:144	a critical element of the human Immunoglobulin G (IgG) constant region	127:196	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	7	33	theme	aggregation	989:999	arg1	behavior					1001:1008	its aggregation behavior	985:1008	its aggregation behavior	985:1008	Moreover, the combination of glycosylation and stabilization completely suppressed its aggregation behavior.					
33915325	3	34	theme	host	433:436	arg1	pastoris					454:461	the host organism Pichia pastoris	429:461	the host organism Pichia pastoris	429:461	Recently, we succeeded in preparing glycosylated and non-glycosylated CH2 domain in the host organism Pichia pastoris.					
33915325	1	35	theme	region	191:196	arg1	domain					117:122	The CH2 domain	109:122	The CH2 domain	109:122	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	1	35	theme	region	191:196	arg1	element					138:144	a critical element	127:144	a critical element of the human Immunoglobulin G (IgG) constant region	127:196	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	8	36	theme	glycosylation	1086:1098	arg1	strategy					1189:1196	a fruitful strategy	1178:1196	a fruitful strategy to generate promising immunoglobulin scaffolds	1178:1243	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	8	36	theme	glycosylation	1086:1098	arg1	combination					1071:1081	the combination	1067:1081	the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein	1067:1167	Since protein aggregation is related to immunogenicity, the combination of glycosylation and stabilization to eliminate the aggregation behavior of a protein could be a fruitful strategy to generate promising immunoglobulin scaffolds.					
33915325	7	37	theme	glycosylation	931:943	arg1	combination					916:926	the combination	912:926	the combination of glycosylation and stabilization	912:961	Moreover, the combination of glycosylation and stabilization completely suppressed its aggregation behavior.					
33915325	3	38	theme	organism	438:445	arg1	pastoris					454:461	the host organism Pichia pastoris	429:461	the host organism Pichia pastoris	429:461	Recently, we succeeded in preparing glycosylated and non-glycosylated CH2 domain in the host organism Pichia pastoris.					
33915325	2	39	theme	novel	315:319	arg1	approaches					333:342	novel therapeutic approaches	315:342	novel therapeutic approaches	315:342	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	2	40	from	domain	243:248	arg1	IgG					253:255	IgG	253:255	IgG	253:255	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	0	41	theme	IgG1	48:51	arg1	domain					32:37	CH2 domain	28:37	CH2 domain of human IgG1	28:51	Abolition of aggregation of CH2 domain of human IgG1 when combining glycosylation and protein stabilization.					
33915325	0	41	theme	IgG1	48:51	arg1	IgG1					48:51	human IgG1	42:51	human IgG1	42:51	Abolition of aggregation of CH2 domain of human IgG1 when combining glycosylation and protein stabilization.					
33915325	2	42	theme	CH2	212:214	arg1	domain					243:248	the least stable domain	226:248	the least stable domain in IgG	226:255	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	2	42	theme	CH2	212:214	arg1	domain					216:221	the CH2 domain	208:221	the CH2 domain	208:221	Although the CH2 domain is the least stable domain in IgG, it is also a promising scaffold candidate for developing novel therapeutic approaches.					
33915325	4	43	gly	glycosylation	489:501	arg1	domain					514:519	the CH2 domain	506:519	the CH2 domain	506:519	Herein, we verified that glycosylation of the CH2 domain decreased both, its tendency to aggregate and its immunogenicity in mice, suggesting that aggregation and immunogenicity are related.					
33915325	1	44	theme	critical	129:136	arg1	domain					117:122	The CH2 domain	109:122	The CH2 domain	109:122	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	1	44	theme	critical	129:136	arg1	element					138:144	a critical element	127:144	a critical element of the human Immunoglobulin G (IgG) constant region	127:196	The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region.					
33915325	6	45	theme	CH2	890:892	arg1	domain					894:899	the CH2 domain	886:899	the CH2 domain	886:899	We found that stabilization alone significantly decreased the aggregation of the CH2 domain.					
32404934	5	0	theme	mucus	775:779	arg1	properties					761:770	the physical properties	748:770	the physical properties of mucus across different pH ranges	748:806	Structural, mutagenic and biophysical data provide insights into how the TFFs recognise this disaccharide and rationalise their ability to modulate the physical properties of mucus across different pH ranges.					
32404934	1	1	theme	peptides	142:149	arg1	host					108:111	a host	106:111	a host of protective disulfide-rich peptides, including the trefoil factors (TFFs)	106:187	The mucosal epithelium secretes a host of protective disulfide-rich peptides, including the trefoil factors (TFFs).					
32404934	4	2	theme	TFF	577:579	arg1	binding					581:587	TFF binding	577:587	TFF binding to mucins	577:597	Degradation of this disaccharide by a glycoside hydrolase abrogates TFF binding to mucins.					
32404934	5	3	theme	Structural	600:609	arg1	data					638:641	Structural, mutagenic and biophysical data	600:641	Structural, mutagenic and biophysical data	600:641	Structural, mutagenic and biophysical data provide insights into how the TFFs recognise this disaccharide and rationalise their ability to modulate the physical properties of mucus across different pH ranges.					
32404934	2	4	theme	cell	254:257	arg1	migration					259:267	cell migration	254:267	cell migration	254:267	The TFFs increase the viscoelasticity of the mucosa and promote cell migration, though the molecular mechanisms underlying these functions have remained poorly defined.					
32404934	3	5	theme	mucin-like	487:496	arg1	O-glycans					498:506	some mucin-like O-glycans	482:506	some mucin-like O-glycans	482:506	Here, we demonstrate that all TFFs are divalent lectins that recognise the GlcNAc-α-1,4-Gal disaccharide, which terminates some mucin-like O-glycans.					
32404934	6	6	theme	glycoproteins	896:908	arg1	state					879:883	the glycosylation state	861:883	the glycosylation state of mucosal glycoproteins	861:908	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	5	7	theme	mutagenic	612:620	arg1	data					638:641	Structural, mutagenic and biophysical data	600:641	Structural, mutagenic and biophysical data	600:641	Structural, mutagenic and biophysical data provide insights into how the TFFs recognise this disaccharide and rationalise their ability to modulate the physical properties of mucus across different pH ranges.					
32404934	5	8	theme	physical	752:759	arg1	properties					761:770	the physical properties	748:770	the physical properties of mucus across different pH ranges	748:806	Structural, mutagenic and biophysical data provide insights into how the TFFs recognise this disaccharide and rationalise their ability to modulate the physical properties of mucus across different pH ranges.					
32404934	6	9	theme	mucosal	888:894	arg1	glycoproteins					896:908	mucosal glycoproteins	888:908	mucosal glycoproteins	888:908	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	5	10	theme	biophysical	626:636	arg1	data					638:641	Structural, mutagenic and biophysical data	600:641	Structural, mutagenic and biophysical data	600:641	Structural, mutagenic and biophysical data provide insights into how the TFFs recognise this disaccharide and rationalise their ability to modulate the physical properties of mucus across different pH ranges.					
32404934	2	11	theme	molecular	281:289	arg1	mechanisms					291:300	the molecular mechanisms	277:300	the molecular mechanisms underlying these functions	277:327	The TFFs increase the viscoelasticity of the mucosa and promote cell migration, though the molecular mechanisms underlying these functions have remained poorly defined.					
32404934	1	12	theme	trefoil	166:172	arg1	TFFs					183:186	TFFs	183:186	TFFs	183:186	The mucosal epithelium secretes a host of protective disulfide-rich peptides, including the trefoil factors (TFFs).					
32404934	1	12	theme	trefoil	166:172	arg1	factors					174:180	the trefoil factors	162:180	the trefoil factors (TFFs)	162:187	The mucosal epithelium secretes a host of protective disulfide-rich peptides, including the trefoil factors (TFFs).					
32404934	0	13	theme	Trefoil	0:6	arg1	factors					8:14	Trefoil factors	0:14	Trefoil factors	0:14	Trefoil factors share a lectin activity that defines their role in mucus.					
32404934	4	14	theme	glycoside	547:555	arg1	hydrolase					557:565	a glycoside hydrolase	545:565	a glycoside hydrolase	545:565	Degradation of this disaccharide by a glycoside hydrolase abrogates TFF binding to mucins.					
32404934	6	15	gly	glycosylation	865:877	arg1	glycoproteins					896:908	mucosal glycoproteins	888:908	mucosal glycoproteins	888:908	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	3	16	theme	divalent	398:405	arg1	lectins					407:413	divalent lectins	398:413	divalent lectins that recognise the GlcNAc-α-1,4-Gal disaccharide, which terminates some mucin-like O-glycans	398:506	Here, we demonstrate that all TFFs are divalent lectins that recognise the GlcNAc-α-1,4-Gal disaccharide, which terminates some mucin-like O-glycans.					
32404934	3	16	theme	divalent	398:405	arg1	TFFs					389:392	all TFFs	385:392	all TFFs	385:392	Here, we demonstrate that all TFFs are divalent lectins that recognise the GlcNAc-α-1,4-Gal disaccharide, which terminates some mucin-like O-glycans.					
32404934	6	17	theme	human	972:976	arg1	proteins					978:985	other human proteins	966:985	other human proteins	966:985	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	1	18	theme	mucosal	78:84	arg1	epithelium					86:95	The mucosal epithelium	74:95	The mucosal epithelium	74:95	The mucosal epithelium secretes a host of protective disulfide-rich peptides, including the trefoil factors (TFFs).					
32404934	3	19	theme	GlcNAc-α-1,4-Gal	434:449	arg1	disaccharide					451:462	the GlcNAc-α-1,4-Gal disaccharide	430:462	the GlcNAc-α-1,4-Gal disaccharide	430:462	Here, we demonstrate that all TFFs are divalent lectins that recognise the GlcNAc-α-1,4-Gal disaccharide, which terminates some mucin-like O-glycans.					
32404934	6	20	theme	other	966:970	arg1	proteins					978:985	other human proteins	966:985	other human proteins	966:985	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	5	21	theme	different	788:796	arg1	ranges					801:806	different pH ranges	788:806	different pH ranges	788:806	Structural, mutagenic and biophysical data provide insights into how the TFFs recognise this disaccharide and rationalise their ability to modulate the physical properties of mucus across different pH ranges.					
32404934	6	22	theme	glycosylation	865:877	arg1	state					879:883	the glycosylation state	861:883	the glycosylation state of mucosal glycoproteins	861:908	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	5	23	theme	pH	798:799	arg1	ranges					801:806	different pH ranges	788:806	different pH ranges	788:806	Structural, mutagenic and biophysical data provide insights into how the TFFs recognise this disaccharide and rationalise their ability to modulate the physical properties of mucus across different pH ranges.					
32404934	0	24	theme	lectin	24:29	arg1	activity					31:38	a lectin activity	22:38	a lectin activity that defines their role in mucus	22:71	Trefoil factors share a lectin activity that defines their role in mucus.					
32404934	0	25	from	role	59:62	arg1	mucus					67:71	mucus	67:71	mucus	67:71	Trefoil factors share a lectin activity that defines their role in mucus.					
32404934	6	26	theme	trefoil	947:953	arg1	domains					955:961	trefoil domains	947:961	trefoil domains in other human proteins	947:985	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	6	27	gly	glycoproteins	896:908	arg1	glycoproteins					896:908	mucosal glycoproteins	888:908	mucosal glycoproteins	888:908	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	4	28	theme	disaccharide	529:540	arg1	Degradation					509:519	Degradation	509:519	Degradation of this disaccharide by a glycoside hydrolase	509:565	Degradation of this disaccharide by a glycoside hydrolase abrogates TFF binding to mucins.					
32404934	6	29	from	domains	955:961	arg1	proteins					978:985	other human proteins	966:985	other human proteins	966:985	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	2	30	theme	mucosa	235:240	arg1	viscoelasticity					212:226	the viscoelasticity	208:226	the viscoelasticity of the mucosa	208:240	The TFFs increase the viscoelasticity of the mucosa and promote cell migration, though the molecular mechanisms underlying these functions have remained poorly defined.					
32404934	6	31	theme	TFF	832:834	arg1	activity					836:843	TFF activity	832:843	TFF activity	832:843	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	1	32	theme	protective	116:125	arg1	factors					174:180	the trefoil factors	162:180	the trefoil factors (TFFs)	162:187	The mucosal epithelium secretes a host of protective disulfide-rich peptides, including the trefoil factors (TFFs).					
32404934	1	32	theme	protective	116:125	arg1	peptides					142:149	protective disulfide-rich peptides	116:149	protective disulfide-rich peptides	116:149	The mucosal epithelium secretes a host of protective disulfide-rich peptides, including the trefoil factors (TFFs).					
32404934	6	33	theme	lectin	927:932	arg1	function					934:941	a lectin function	925:941	a lectin function for trefoil domains in other human proteins	925:985	These data reveal that TFF activity is dependent on the glycosylation state of mucosal glycoproteins and alludes to a lectin function for trefoil domains in other human proteins.					
32404934	1	34	theme	disulfide-rich	127:140	arg1	factors					174:180	the trefoil factors	162:180	the trefoil factors (TFFs)	162:187	The mucosal epithelium secretes a host of protective disulfide-rich peptides, including the trefoil factors (TFFs).					
32404934	1	34	theme	disulfide-rich	127:140	arg1	peptides					142:149	protective disulfide-rich peptides	116:149	protective disulfide-rich peptides	116:149	The mucosal epithelium secretes a host of protective disulfide-rich peptides, including the trefoil factors (TFFs).					
33977722	0	0	from	Modification	30:41	arg1	Fucose					55:60	the Core Fucose	46:60	the Core Fucose	46:60	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody Enhances Its Fcγ Receptor Affinity and ADCC Activity.					
33977722	9	1	theme	site-selective	1735:1748	arg1	modification					1750:1761	the direct site-selective modification	1724:1761	the direct site-selective modification	1724:1761	Thus, the direct site-selective modification turns the adverse effect of the core fucose into a favorable force to promote the carbohydrate-carbohydrate interactions.					
33977722	2	2	theme	antibody	354:361	arg1	N-glycans					338:346	the Fc domain N-glycans	324:346	the Fc domain N-glycans of an antibody	324:361	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	1	3	theme	anti-inflammatory	245:261	arg1	activities					263:272	an antibody's pro- or anti-inflammatory activities	223:272	an antibody's pro- or anti-inflammatory activities	223:272	Fc glycosylation profoundly impacts the effector functions of antibodies and often dictates an antibody's pro- or anti-inflammatory activities.					
33977722	2	4	gly	fucosylation	308:319	arg1	N-glycans					338:346	the Fc domain N-glycans	324:346	the Fc domain N-glycans of an antibody	324:361	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	5	5	with	transfucosylation	954:970	arg1	mutant					996:1001	a novel fucosidase mutant	977:1001	a novel fucosidase mutant	977:1001	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	5	5	with	transfucosylation	954:970	arg1	able					1026:1029	able	1026:1029	able	1026:1029	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	5	6	theme	enzymatic	944:952	arg1	transfucosylation					954:970	an enzymatic transfucosylation	941:970	an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate	941:1087	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	6	7	theme	core	1123:1126	arg1	l-fucose					1128:1135	the core l-fucose	1119:1135	the core l-fucose	1119:1135	We found that replacement of the core l-fucose with 6-azide- or 6-hydroxy-l-fucose (l-galactose) significantly enhanced the antibody's affinity for FcγRIIIa receptors and substantially increased the ADCC activity.					
33977722	0	8	theme	Receptor	94:101	arg1	Affinity					103:110	Its Fcγ Receptor Affinity	86:110	Its Fcγ Receptor Affinity	86:110	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody Enhances Its Fcγ Receptor Affinity and ADCC Activity.					
33977722	7	9	theme	molecular	1399:1407	arg1	simulations					1418:1428	molecular dynamics simulations	1399:1428	molecular dynamics simulations	1399:1428	To understand the mechanism of the modified fucose-mediated affinity enhancement, we performed molecular dynamics simulations.					
33977722	9	10	theme	direct	1728:1733	arg1	modification					1750:1761	the direct site-selective modification	1724:1761	the direct site-selective modification	1724:1761	Thus, the direct site-selective modification turns the adverse effect of the core fucose into a favorable force to promote the carbohydrate-carbohydrate interactions.					
33977722	4	11	theme	site-specific	773:785	arg1	modification					787:798	special site-specific modification	765:798	special site-specific modification on the core fucose	765:817	We report here that in contrast to natural core fucose, special site-specific modification on the core fucose could dramatically enhance the affinity of an antibody for FcγRIIIa.					
33977722	0	12	theme	Fcγ	90:92	arg1	Affinity					103:110	Its Fcγ Receptor Affinity	86:110	Its Fcγ Receptor Affinity	86:110	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody Enhances Its Fcγ Receptor Affinity and ADCC Activity.					
33977722	0	13	dep	Affinity	103:110	arg1	Activity					121:128	Activity	121:128	Activity	121:128	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody Enhances Its Fcγ Receptor Affinity and ADCC Activity.					
33977722	2	14	theme	Fc	328:329	arg1	N-glycans					338:346	the Fc domain N-glycans	324:346	the Fc domain N-glycans of an antibody	324:361	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	6	15	theme	l-fucose	1128:1135	arg1	replacement					1104:1114	replacement	1104:1114	replacement of the core l-fucose with 6-azide- or 6-hydroxy-l-fucose (l-galactose)	1104:1185	We found that replacement of the core l-fucose with 6-azide- or 6-hydroxy-l-fucose (l-galactose) significantly enhanced the antibody's affinity for FcγRIIIa receptors and substantially increased the ADCC activity.					
33977722	4	16	theme	special	765:771	arg1	modification					787:798	special site-specific modification	765:798	special site-specific modification on the core fucose	765:817	We report here that in contrast to natural core fucose, special site-specific modification on the core fucose could dramatically enhance the affinity of an antibody for FcγRIIIa.					
33977722	3	17	theme	fucose	545:550	arg1	presence					526:533	the presence	522:533	the presence of a core fucose	522:550	Previous structural studies have suggested that the presence of a core fucose remarkably decreases the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans, leading to reduced affinity.					
33977722	1	18	theme	effector	171:178	arg1	functions					180:188	the effector functions	167:188	the effector functions of antibodies	167:202	Fc glycosylation profoundly impacts the effector functions of antibodies and often dictates an antibody's pro- or anti-inflammatory activities.					
33977722	8	19	theme	selective	1540:1548	arg1	modifications					1562:1574	the selective core-fucose modifications	1536:1574	the selective core-fucose modifications	1536:1574	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	8	20	theme	carbohydrate-carbohydrate	1610:1634	arg1	interactions					1636:1647	unique carbohydrate-carbohydrate interactions	1603:1647	unique carbohydrate-carbohydrate interactions	1603:1647	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	4	21	from	modification	787:798	arg1	fucose					812:817	the core fucose	803:817	the core fucose	803:817	We report here that in contrast to natural core fucose, special site-specific modification on the core fucose could dramatically enhance the affinity of an antibody for FcγRIIIa.					
33977722	4	22	theme	core	807:810	arg1	fucose					812:817	the core fucose	803:817	the core fucose	803:817	We report here that in contrast to natural core fucose, special site-specific modification on the core fucose could dramatically enhance the affinity of an antibody for FcγRIIIa.					
33977722	7	23	theme	dynamics	1409:1416	arg1	simulations					1418:1428	molecular dynamics simulations	1399:1428	molecular dynamics simulations	1399:1428	To understand the mechanism of the modified fucose-mediated affinity enhancement, we performed molecular dynamics simulations.					
33977722	0	24	theme	Chemoenzymatic	15:28	arg1	Modification					30:41	Site-Selective Chemoenzymatic Modification	0:41	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody	0:75	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody Enhances Its Fcγ Receptor Affinity and ADCC Activity.					
33977722	8	25	theme	unique	1603:1608	arg1	interactions					1636:1647	unique carbohydrate-carbohydrate interactions	1603:1647	unique carbohydrate-carbohydrate interactions	1603:1647	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	3	26	theme	favorable	588:596	arg1	interactions					624:635	the unique and favorable carbohydrate-carbohydrate interactions	573:635	the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans	573:677	Previous structural studies have suggested that the presence of a core fucose remarkably decreases the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans, leading to reduced affinity.					
33977722	0	27	theme	Site-Selective	0:13	arg1	Modification					30:41	Site-Selective Chemoenzymatic Modification	0:41	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody	0:75	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody Enhances Its Fcγ Receptor Affinity and ADCC Activity.					
33977722	4	28	theme	core	752:755	arg1	fucose					757:762	natural core fucose	744:762	natural core fucose	744:762	We report here that in contrast to natural core fucose, special site-specific modification on the core fucose could dramatically enhance the affinity of an antibody for FcγRIIIa.					
33977722	5	29	theme	site-selective	892:905	arg1	modification					907:918	The site-selective modification	888:918	The site-selective modification	888:918	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	3	30	theme	carbohydrate-carbohydrate	598:622	arg1	interactions					624:635	the unique and favorable carbohydrate-carbohydrate interactions	573:635	the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans	573:677	Previous structural studies have suggested that the presence of a core fucose remarkably decreases the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans, leading to reduced affinity.					
33977722	1	31	theme	antibodies	193:202	arg1	functions					180:188	the effector functions	167:188	the effector functions of antibodies	167:202	Fc glycosylation profoundly impacts the effector functions of antibodies and often dictates an antibody's pro- or anti-inflammatory activities.					
33977722	8	32	theme	interactions	1636:1647	arg1	importance					1589:1598	the importance	1585:1598	the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies	1585:1715	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	9	33	theme	core	1795:1798	arg1	fucose					1800:1805	the core fucose	1791:1805	the core fucose	1791:1805	Thus, the direct site-selective modification turns the adverse effect of the core fucose into a favorable force to promote the carbohydrate-carbohydrate interactions.					
33977722	5	34	theme	fucosidase	985:994	arg1	mutant					996:1001	a novel fucosidase mutant	977:1001	a novel fucosidase mutant	977:1001	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	5	34	theme	fucosidase	985:994	arg1	able					1026:1029	able	1026:1029	able	1026:1029	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	3	35	theme	unique	577:582	arg1	interactions					624:635	the unique and favorable carbohydrate-carbohydrate interactions	573:635	the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans	573:677	Previous structural studies have suggested that the presence of a core fucose remarkably decreases the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans, leading to reduced affinity.					
33977722	2	36	theme	cellular	444:451	arg1	ADCC					467:470	ADCC	467:470	ADCC	467:470	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	2	36	theme	cellular	444:451	arg1	cytotoxicity					453:464	antibody-dependent cellular cytotoxicity	425:464	antibody-dependent cellular cytotoxicity (ADCC)	425:471	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	8	37	theme	high	1662:1665	arg1	affinity					1676:1683	high FcγRIIIa affinity	1662:1683	high FcγRIIIa affinity	1662:1683	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	9	38	theme	fucose	1800:1805	arg1	effect					1781:1786	the adverse effect	1769:1786	the adverse effect of the core fucose into a favorable force	1769:1828	Thus, the direct site-selective modification turns the adverse effect of the core fucose into a favorable force to promote the carbohydrate-carbohydrate interactions.					
33977722	6	39	with	replacement	1104:1114	arg1	6-hydroxy-l-fucose					1154:1171	6-hydroxy-l-fucose	1154:1171	6-hydroxy-l-fucose (l-galactose)	1154:1185	We found that replacement of the core l-fucose with 6-azide- or 6-hydroxy-l-fucose (l-galactose) significantly enhanced the antibody's affinity for FcγRIIIa receptors and substantially increased the ADCC activity.					
33977722	6	39	with	replacement	1104:1114	arg1	6-azide-					1142:1149	6-azide-	1142:1149	6-azide-	1142:1149	We found that replacement of the core l-fucose with 6-azide- or 6-hydroxy-l-fucose (l-galactose) significantly enhanced the antibody's affinity for FcγRIIIa receptors and substantially increased the ADCC activity.					
33977722	6	39	with	replacement	1104:1114	arg1	l-galactose					1174:1184	l-galactose	1174:1184	l-galactose	1174:1184	We found that replacement of the core l-fucose with 6-azide- or 6-hydroxy-l-fucose (l-galactose) significantly enhanced the antibody's affinity for FcγRIIIa receptors and substantially increased the ADCC activity.					
33977722	2	40	theme	core	303:306	arg1	fucosylation					308:319	core fucosylation	303:319	core fucosylation of the Fc domain N-glycans of an antibody	303:361	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	2	41	theme	antibody-dependent	425:442	arg1	ADCC					467:470	ADCC	467:470	ADCC	467:470	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	2	41	theme	antibody-dependent	425:442	arg1	cytotoxicity					453:464	antibody-dependent cellular cytotoxicity	425:464	antibody-dependent cellular cytotoxicity (ADCC)	425:471	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	0	42	theme	Core	50:53	arg1	Fucose					55:60	the Core Fucose	46:60	the Core Fucose	46:60	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody Enhances Its Fcγ Receptor Affinity and ADCC Activity.					
33977722	7	43	theme	modified	1339:1346	arg1	enhancement					1373:1383	the modified fucose-mediated affinity enhancement	1335:1383	the modified fucose-mediated affinity enhancement	1335:1383	To understand the mechanism of the modified fucose-mediated affinity enhancement, we performed molecular dynamics simulations.					
33977722	3	44	theme	reduced	691:697	arg1	affinity					699:706	reduced affinity	691:706	reduced affinity	691:706	Previous structural studies have suggested that the presence of a core fucose remarkably decreases the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans, leading to reduced affinity.					
33977722	8	45	theme	glycan	1468:1473	arg1	contacts					1475:1482	glycan contacts	1468:1482	glycan contacts between the Fc and the Fc receptor	1468:1517	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	7	46	theme	fucose-mediated	1348:1362	arg1	enhancement					1373:1383	the modified fucose-mediated affinity enhancement	1335:1383	the modified fucose-mediated affinity enhancement	1335:1383	To understand the mechanism of the modified fucose-mediated affinity enhancement, we performed molecular dynamics simulations.					
33977722	2	47	theme	FcγRIIIa	402:409	arg1	receptors					411:419	FcγRIIIa receptors	402:419	FcγRIIIa receptors	402:419	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	6	48	theme	ADCC	1289:1292	arg1	activity					1294:1301	the ADCC activity	1285:1301	the ADCC activity	1285:1301	We found that replacement of the core l-fucose with 6-azide- or 6-hydroxy-l-fucose (l-galactose) significantly enhanced the antibody's affinity for FcγRIIIa receptors and substantially increased the ADCC activity.					
33977722	3	49	theme	Previous	474:481	arg1	studies					494:500	Previous structural studies	474:500	Previous structural studies	474:500	Previous structural studies have suggested that the presence of a core fucose remarkably decreases the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans, leading to reduced affinity.					
33977722	8	50	theme	FcγRIIIa	1667:1674	arg1	affinity					1676:1683	high FcγRIIIa affinity	1662:1683	high FcγRIIIa affinity	1662:1683	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	9	51	theme	favorable	1814:1822	arg1	force					1824:1828	a favorable force	1812:1828	a favorable force	1812:1828	Thus, the direct site-selective modification turns the adverse effect of the core fucose into a favorable force to promote the carbohydrate-carbohydrate interactions.					
33977722	4	52	theme	antibody	865:872	arg1	affinity					850:857	the affinity	846:857	the affinity of an antibody for FcγRIIIa	846:885	We report here that in contrast to natural core fucose, special site-specific modification on the core fucose could dramatically enhance the affinity of an antibody for FcγRIIIa.					
33977722	4	53	theme	natural	744:750	arg1	fucose					757:762	natural core fucose	744:762	natural core fucose	744:762	We report here that in contrast to natural core fucose, special site-specific modification on the core fucose could dramatically enhance the affinity of an antibody for FcγRIIIa.					
33977722	8	54	theme	ADCC	1689:1692	arg1	activity					1694:1701	ADCC activity	1689:1701	ADCC activity of antibodies	1689:1715	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	5	55	theme	donor	1073:1077	arg1	substrate					1079:1087	the donor substrate	1069:1087	the donor substrate	1069:1087	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	5	55	theme	donor	1073:1077	arg1	fluoride					1057:1064	modified α-fucosyl fluoride	1038:1064	modified α-fucosyl fluoride	1038:1064	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	2	56	theme	N-glycans	338:346	arg1	fucosylation					308:319	core fucosylation	303:319	core fucosylation of the Fc domain N-glycans of an antibody	303:361	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	8	57	theme	Fc	1507:1508	arg1	receptor					1510:1517	the Fc and the Fc receptor	1492:1517	receptor	1510:1517	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	6	58	theme	FcγRIIIa	1238:1245	arg1	receptors					1247:1255	FcγRIIIa receptors	1238:1255	FcγRIIIa receptors	1238:1255	We found that replacement of the core l-fucose with 6-azide- or 6-hydroxy-l-fucose (l-galactose) significantly enhanced the antibody's affinity for FcγRIIIa receptors and substantially increased the ADCC activity.					
33977722	3	59	theme	receptor	660:667	arg1	N-glycans					669:677	the receptor N-glycans	656:677	the receptor N-glycans	656:677	Previous structural studies have suggested that the presence of a core fucose remarkably decreases the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans, leading to reduced affinity.					
33977722	4	60	dep	fucose	757:762	arg1	contrast					732:739	contrast	732:739	contrast	732:739	We report here that in contrast to natural core fucose, special site-specific modification on the core fucose could dramatically enhance the affinity of an antibody for FcγRIIIa.					
33977722	2	61	theme	domain	331:336	arg1	N-glycans					338:346	the Fc domain N-glycans	324:346	the Fc domain N-glycans of an antibody	324:361	It is well established that core fucosylation of the Fc domain N-glycans of an antibody significantly reduces its affinity for FcγRIIIa receptors and antibody-dependent cellular cytotoxicity (ADCC).					
33977722	8	62	theme	core-fucose	1550:1560	arg1	modifications					1562:1574	the selective core-fucose modifications	1536:1574	the selective core-fucose modifications	1536:1574	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	1	63	theme	Fc	131:132	arg1	glycosylation					134:146	Fc glycosylation	131:146	Fc glycosylation	131:146	Fc glycosylation profoundly impacts the effector functions of antibodies and often dictates an antibody's pro- or anti-inflammatory activities.					
33977722	5	64	theme	novel	979:983	arg1	mutant					996:1001	a novel fucosidase mutant	977:1001	a novel fucosidase mutant	977:1001	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	5	64	theme	novel	979:983	arg1	able					1026:1029	able	1026:1029	able	1026:1029	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	0	65	theme	Antibody	68:75	arg1	Modification					30:41	Site-Selective Chemoenzymatic Modification	0:41	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody	0:75	Site-Selective Chemoenzymatic Modification on the Core Fucose of an Antibody Enhances Its Fcγ Receptor Affinity and ADCC Activity.					
33977722	7	66	theme	affinity	1364:1371	arg1	enhancement					1373:1383	the modified fucose-mediated affinity enhancement	1335:1383	the modified fucose-mediated affinity enhancement	1335:1383	To understand the mechanism of the modified fucose-mediated affinity enhancement, we performed molecular dynamics simulations.					
33977722	5	67	theme	modified	1038:1045	arg1	substrate					1079:1087	the donor substrate	1069:1087	the donor substrate	1069:1087	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	5	67	theme	modified	1038:1045	arg1	fluoride					1057:1064	modified α-fucosyl fluoride	1038:1064	modified α-fucosyl fluoride	1038:1064	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	3	68	theme	structural	483:492	arg1	studies					494:500	Previous structural studies	474:500	Previous structural studies	474:500	Previous structural studies have suggested that the presence of a core fucose remarkably decreases the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans, leading to reduced affinity.					
33977722	8	69	theme	contacts	1475:1482	arg1	number					1458:1463	the number	1454:1463	the number of glycan contacts between the Fc and the Fc receptor	1454:1517	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	7	70	theme	enhancement	1373:1383	arg1	mechanism					1322:1330	the mechanism	1318:1330	the mechanism of the modified fucose-mediated affinity enhancement	1318:1383	To understand the mechanism of the modified fucose-mediated affinity enhancement, we performed molecular dynamics simulations.					
33977722	1	71	theme	pro-	237:240	arg1	activities					263:272	an antibody's pro- or anti-inflammatory activities	223:272	an antibody's pro- or anti-inflammatory activities	223:272	Fc glycosylation profoundly impacts the effector functions of antibodies and often dictates an antibody's pro- or anti-inflammatory activities.					
33977722	9	72	theme	carbohydrate-carbohydrate	1845:1869	arg1	interactions					1871:1882	the carbohydrate-carbohydrate interactions	1841:1882	the carbohydrate-carbohydrate interactions	1841:1882	Thus, the direct site-selective modification turns the adverse effect of the core fucose into a favorable force to promote the carbohydrate-carbohydrate interactions.					
33977722	9	73	theme	adverse	1773:1779	arg1	effect					1781:1786	the adverse effect	1769:1786	the adverse effect of the core fucose into a favorable force	1769:1828	Thus, the direct site-selective modification turns the adverse effect of the core fucose into a favorable force to promote the carbohydrate-carbohydrate interactions.					
33977722	5	74	theme	α-fucosyl	1047:1055	arg1	substrate					1079:1087	the donor substrate	1069:1087	the donor substrate	1069:1087	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	5	74	theme	α-fucosyl	1047:1055	arg1	fluoride					1057:1064	modified α-fucosyl fluoride	1038:1064	modified α-fucosyl fluoride	1038:1064	The site-selective modification was achieved through an enzymatic transfucosylation with a novel fucosidase mutant, which was shown to be able to use modified α-fucosyl fluoride as the donor substrate.					
33977722	3	75	theme	core	540:543	arg1	fucose					545:550	a core fucose	538:550	a core fucose	538:550	Previous structural studies have suggested that the presence of a core fucose remarkably decreases the unique and favorable carbohydrate-carbohydrate interactions between the Fc and the receptor N-glycans, leading to reduced affinity.					
33977722	8	76	theme	antibodies	1706:1715	arg1	affinity					1676:1683	high FcγRIIIa affinity	1662:1683	high FcγRIIIa affinity	1662:1683	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
33977722	8	76	theme	antibodies	1706:1715	arg1	activity					1694:1701	ADCC activity	1689:1701	ADCC activity of antibodies	1689:1715	Our data revealed that the number of glycan contacts between the Fc and the Fc receptor was increased by the selective core-fucose modifications, showing the importance of unique carbohydrate-carbohydrate interactions in achieving high FcγRIIIa affinity and ADCC activity of antibodies.					
32285041	5	0	theme	different	884:892	arg1	therapeutics					910:921	four different glycoengineered therapeutics	879:921	four different glycoengineered therapeutics (i.e., Rituximab, erythropoietin, Enbrel, and alpha-1 antitrypsin) produced in CHO cells	879:1010	We apply our methodology to four different glycoengineered therapeutics (i.e., Rituximab, erythropoietin, Enbrel, and alpha-1 antitrypsin) produced in CHO cells.					
32285041	5	1	theme	CHO	1002:1004	arg1	cells					1006:1010	CHO cells	1002:1010	CHO cells	1002:1010	We apply our methodology to four different glycoengineered therapeutics (i.e., Rituximab, erythropoietin, Enbrel, and alpha-1 antitrypsin) produced in CHO cells.					
32285041	7	2	theme	mutations	1390:1398	arg1	outcome					1348:1354	the outcome	1344:1354	the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics	1344:1429	By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics.					
32285041	5	3	theme	glycoengineered	894:908	arg1	therapeutics					910:921	four different glycoengineered therapeutics	879:921	four different glycoengineered therapeutics (i.e., Rituximab, erythropoietin, Enbrel, and alpha-1 antitrypsin) produced in CHO cells	879:1010	We apply our methodology to four different glycoengineered therapeutics (i.e., Rituximab, erythropoietin, Enbrel, and alpha-1 antitrypsin) produced in CHO cells.					
32285041	1	4	from	market	197:202	arg1	important					158:166	important	158:166	important	158:166	Glycosylated biopharmaceuticals are important in the global pharmaceutical market.					
32285041	6	5	theme	mutations	1151:1159	arg1	impact					1121:1126	the impact	1117:1126	the impact of glycosyltransferase mutations on reactions catalyzed by other glycosyltransferases	1117:1212	Our model accurately predicted N-linked glycosylation following glycoengineering and further quantified the impact of glycosyltransferase mutations on reactions catalyzed by other glycosyltransferases.					
32285041	4	6	theme	glycosyltransferase	590:608	arg1	specificity					615:625	putative glycosyltransferase (GT) specificity	581:625	putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans	581:686	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	5	7	dep	Rituximab	930:938	arg1	i.e.					924:927	i.e.	924:927	i.e.	924:927	We apply our methodology to four different glycoengineered therapeutics (i.e., Rituximab, erythropoietin, Enbrel, and alpha-1 antitrypsin) produced in CHO cells.					
32285041	6	8	theme	glycosyltransferase	1131:1149	arg1	mutations					1151:1159	glycosyltransferase mutations	1131:1159	glycosyltransferase mutations	1131:1159	Our model accurately predicted N-linked glycosylation following glycoengineering and further quantified the impact of glycosyltransferase mutations on reactions catalyzed by other glycosyltransferases.					
32285041	7	9	theme	diverse	1407:1413	arg1	biotherapeutics					1415:1429	the diverse biotherapeutics	1403:1429	the diverse biotherapeutics	1403:1429	By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics.					
32285041	7	10	from	outcome	1348:1354	arg1	biotherapeutics					1415:1429	the diverse biotherapeutics	1403:1429	the diverse biotherapeutics	1403:1429	By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics.					
32285041	8	11	theme	biopharmaceutical	1583:1599	arg1	research					1601:1608	biopharmaceutical research	1583:1608	biopharmaceutical research	1583:1608	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	4	12	theme	measured	671:678	arg1	glycans					680:686	all measured glycans	667:686	all measured glycans	667:686	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	3	13	theme	glycosyltransferase	402:420	arg1	specificity					422:432	glycosyltransferase specificity	402:432	glycosyltransferase specificity	402:432	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	4	14	theme	chain	704:708	arg1	modelling					710:718	the Markov chain modelling	693:718	the Markov chain modelling	693:718	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	6	15	link	N-linked	1044:1051	arg1	glycosylation					1053:1065	N-linked glycosylation	1044:1065	N-linked glycosylation	1044:1065	Our model accurately predicted N-linked glycosylation following glycoengineering and further quantified the impact of glycosyltransferase mutations on reactions catalyzed by other glycosyltransferases.					
32285041	3	16	theme	N-linked	509:516	arg1	process					552:558	a Markov process	543:558	a Markov process	543:558	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	3	16	theme	N-linked	509:516	arg1	glycosylation					526:538	N-linked protein glycosylation	509:538	N-linked protein glycosylation	509:538	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	3	17	theme	specificity	422:432	arg1	discovery					389:397	discovery	389:397	discovery of glycosyltransferase specificity	389:432	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	7	18	theme	single	1281:1286	arg1	mutants					1308:1314	single glycosyltransferase mutants	1281:1314	single glycosyltransferase mutants	1281:1314	By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics.					
32285041	3	19	theme	protein	518:524	arg1	process					552:558	a Markov process	543:558	a Markov process	543:558	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	3	19	theme	protein	518:524	arg1	glycosylation					526:538	N-linked protein glycosylation	509:538	N-linked protein glycosylation	509:538	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	0	20	theme	Markov	2:7	arg1	model					9:13	A Markov model	0:13	A Markov model of glycosylation	0:30	A Markov model of glycosylation elucidates isozyme specificity and glycosyltransferase interactions for glycoengineering.					
32285041	8	21	theme	genetic	1673:1679	arg1	basis					1681:1685	the genetic basis	1669:1685	the genetic basis of complex changes in glycosylation	1669:1721	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	3	22	link	N-linked	509:516	arg1	process					552:558	a Markov process	543:558	a Markov process	543:558	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	3	22	link	N-linked	509:516	arg1	glycosylation					526:538	N-linked protein glycosylation	509:538	N-linked protein glycosylation	509:538	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	8	23	theme	broader	1625:1631	arg1	study					1633:1637	the broader study	1621:1637	the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation	1621:1721	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	7	24	theme	multi-gene	1359:1368	arg1	mutations					1390:1398	multi-gene glycosyltransferase mutations	1359:1398	multi-gene glycosyltransferase mutations	1359:1398	By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics.					
32285041	4	25	theme	isoform	757:763	arg1	activities					765:774	glycosyltransferase isoform activities	737:774	glycosyltransferase isoform activities	737:774	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	7	26	theme	glycosyltransferase	1370:1388	arg1	mutations					1390:1398	multi-gene glycosyltransferase mutations	1359:1398	multi-gene glycosyltransferase mutations	1359:1398	By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics.					
32285041	8	27	theme	modeling	1443:1450	arg1	approach					1452:1459	this modeling approach	1438:1459	this modeling approach	1438:1459	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	2	28	theme	attribute	364:372	arg1	controllability					323:337	controllability	323:337	controllability of this critical quality attribute	323:372	Despite the importance of their glycan structures, our limited knowledge of the glycosylation machinery still hinders controllability of this critical quality attribute.					
32285041	8	29	theme	rational	1469:1476	arg1	glycoengineering					1478:1493	rational glycoengineering	1469:1493	rational glycoengineering	1469:1493	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	4	30	theme	glycosyltransferase	737:755	arg1	activities					765:774	glycosyltransferase isoform activities	737:774	glycosyltransferase isoform activities	737:774	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	3	31	theme	glycoengineering	446:461	arg1	efforts					463:469	glycoengineering efforts	446:469	glycoengineering efforts	446:469	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	8	32	theme	complex	1690:1696	arg1	changes					1698:1704	complex changes	1690:1704	complex changes in glycosylation	1690:1721	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	7	33	theme	GT-GT	1241:1245	arg1	rules					1259:1263	these learned GT-GT interaction rules	1227:1263	these learned GT-GT interaction rules identified from single glycosyltransferase mutants	1227:1314	By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics.					
32285041	1	34	from	important	158:166	arg1	market					197:202	the global pharmaceutical market	171:202	the global pharmaceutical market	171:202	Glycosylated biopharmaceuticals are important in the global pharmaceutical market.					
32285041	2	35	theme	quality	356:362	arg1	attribute					364:372	this critical quality attribute	342:372	this critical quality attribute	342:372	Despite the importance of their glycan structures, our limited knowledge of the glycosylation machinery still hinders controllability of this critical quality attribute.					
32285041	7	36	theme	interaction	1247:1257	arg1	rules					1259:1263	these learned GT-GT interaction rules	1227:1263	these learned GT-GT interaction rules identified from single glycosyltransferase mutants	1227:1314	By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics.					
32285041	2	37	theme	critical	347:354	arg1	attribute					364:372	this critical quality attribute	342:372	this critical quality attribute	342:372	Despite the importance of their glycan structures, our limited knowledge of the glycosylation machinery still hinders controllability of this critical quality attribute.					
32285041	8	38	from	basis	1681:1685	arg1	glycosylation					1709:1721	glycosylation	1709:1721	glycosylation	1709:1721	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	3	39	theme	Markov	545:550	arg1	process					552:558	a Markov process	543:558	a Markov process	543:558	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	3	39	theme	Markov	545:550	arg1	glycosylation					526:538	N-linked protein glycosylation	509:538	N-linked protein glycosylation	509:538	To facilitate discovery of glycosyltransferase specificity and predict glycoengineering efforts, here we extend the approach to model N-linked protein glycosylation as a Markov process.					
32285041	4	40	theme	biosynthetic	641:652	arg1	pathways					654:661	the biosynthetic pathways	637:661	the biosynthetic pathways for all measured glycans	637:686	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	6	41	from	impact	1121:1126	arg1	reactions					1164:1172	reactions	1164:1172	reactions catalyzed by other glycosyltransferases	1164:1212	Our model accurately predicted N-linked glycosylation following glycoengineering and further quantified the impact of glycosyltransferase mutations on reactions catalyzed by other glycosyltransferases.					
32285041	8	42	theme	changes	1698:1704	arg1	basis					1681:1685	the genetic basis	1669:1685	the genetic basis of complex changes in glycosylation	1669:1721	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	0	43	theme	glycosylation	18:30	arg1	model					9:13	A Markov model	0:13	A Markov model of glycosylation	0:30	A Markov model of glycosylation elucidates isozyme specificity and glycosyltransferase interactions for glycoengineering.					
32285041	7	44	theme	learned	1233:1239	arg1	rules					1259:1263	these learned GT-GT interaction rules	1227:1263	these learned GT-GT interaction rules identified from single glycosyltransferase mutants	1227:1314	By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics.					
32285041	8	45	theme	glycosylation	1642:1654	arg1	study					1633:1637	the broader study	1621:1637	the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation	1621:1721	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	5	46	theme	alpha-1	969:975	arg1	antitrypsin					977:987	alpha-1 antitrypsin	969:987	alpha-1 antitrypsin	969:987	We apply our methodology to four different glycoengineered therapeutics (i.e., Rituximab, erythropoietin, Enbrel, and alpha-1 antitrypsin) produced in CHO cells.					
32285041	4	47	theme	GT	611:612	arg1	specificity					615:625	putative glycosyltransferase (GT) specificity	581:625	putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans	581:686	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	8	48	dep	enables	1461:1467	arg1	aiding					1614:1619	aiding	1614:1619	aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation	1614:1721	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	8	48	dep	enables	1461:1467	arg1	facilitating					1570:1581	facilitating	1570:1581	facilitating biopharmaceutical research	1570:1608	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	2	49	theme	limited	260:266	arg1	knowledge					268:276	our limited knowledge	256:276	our limited knowledge of the glycosylation machinery	256:307	Despite the importance of their glycan structures, our limited knowledge of the glycosylation machinery still hinders controllability of this critical quality attribute.					
32285041	4	50	theme	glycosyltransferase	812:830	arg1	knock-in/knockout					832:848	glycosyltransferase knock-in/knockout	812:848	glycosyltransferase knock-in/knockout	812:848	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	0	51	theme	isozyme	43:49	arg1	specificity					51:61	isozyme specificity	43:61	isozyme specificity	43:61	A Markov model of glycosylation elucidates isozyme specificity and glycosyltransferase interactions for glycoengineering.					
32285041	6	52	theme	N-linked	1044:1051	arg1	glycosylation					1053:1065	N-linked glycosylation	1044:1065	N-linked glycosylation	1044:1065	Our model accurately predicted N-linked glycosylation following glycoengineering and further quantified the impact of glycosyltransferase mutations on reactions catalyzed by other glycosyltransferases.					
32285041	7	53	theme	glycosyltransferase	1288:1306	arg1	mutants					1308:1314	single glycosyltransferase mutants	1281:1314	single glycosyltransferase mutants	1281:1314	By applying these learned GT-GT interaction rules identified from single glycosyltransferase mutants, our model further predicts the outcome of multi-gene glycosyltransferase mutations on the diverse biotherapeutics.					
32285041	8	54	from	changes	1698:1704	arg1	glycosylation					1709:1721	glycosylation	1709:1721	glycosylation	1709:1721	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	2	55	theme	structures	244:253	arg1	importance					217:226	the importance	213:226	the importance of their glycan structures	213:253	Despite the importance of their glycan structures, our limited knowledge of the glycosylation machinery still hinders controllability of this critical quality attribute.					
32285041	1	56	theme	Glycosylated	122:133	arg1	biopharmaceuticals					135:152	Glycosylated biopharmaceuticals	122:152	Glycosylated biopharmaceuticals	122:152	Glycosylated biopharmaceuticals are important in the global pharmaceutical market.					
32285041	6	57	theme	other	1187:1191	arg1	glycosyltransferases					1193:1212	other glycosyltransferases	1187:1212	other glycosyltransferases	1187:1212	Our model accurately predicted N-linked glycosylation following glycoengineering and further quantified the impact of glycosyltransferase mutations on reactions catalyzed by other glycosyltransferases.					
32285041	2	58	theme	glycan	237:242	arg1	structures					244:253	their glycan structures	231:253	their glycan structures	231:253	Despite the importance of their glycan structures, our limited knowledge of the glycosylation machinery still hinders controllability of this critical quality attribute.					
32285041	2	59	theme	machinery	299:307	arg1	knowledge					268:276	our limited knowledge	256:276	our limited knowledge of the glycosylation machinery	256:307	Despite the importance of their glycan structures, our limited knowledge of the glycosylation machinery still hinders controllability of this critical quality attribute.					
32285041	8	60	theme	relationships	1518:1530	arg1	glycoengineering					1478:1493	rational glycoengineering	1469:1493	rational glycoengineering	1469:1493	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	8	60	theme	relationships	1518:1530	arg1	elucidation					1503:1513	the elucidation	1499:1513	the elucidation of relationships between glycosyltransferases	1499:1559	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	0	61	theme	glycosyltransferase	67:85	arg1	interactions					87:98	glycosyltransferase interactions	67:98	glycosyltransferase interactions	67:98	A Markov model of glycosylation elucidates isozyme specificity and glycosyltransferase interactions for glycoengineering.					
32285041	2	62	theme	glycosylation	285:297	arg1	machinery					299:307	the glycosylation machinery	281:307	the glycosylation machinery	281:307	Despite the importance of their glycan structures, our limited knowledge of the glycosylation machinery still hinders controllability of this critical quality attribute.					
32285041	4	63	used	used	723:726	arg2	modelling					710:718	the Markov chain modelling	693:718	the Markov chain modelling	693:718	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	1	64	theme	global	175:180	arg1	market					197:202	the global pharmaceutical market	171:202	the global pharmaceutical market	171:202	Glycosylated biopharmaceuticals are important in the global pharmaceutical market.					
32285041	4	65	theme	Markov	697:702	arg1	modelling					710:718	the Markov chain modelling	693:718	the Markov chain modelling	693:718	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	8	66	from	glycosylation	1709:1721	arg1	basis					1681:1685	the genetic basis	1669:1685	the genetic basis of complex changes in glycosylation	1669:1721	Thus, this modeling approach enables rational glycoengineering and the elucidation of relationships between glycosyltransferases, thereby facilitating biopharmaceutical research and aiding the broader study of glycosylation to elucidate the genetic basis of complex changes in glycosylation.					
32285041	4	67	theme	putative	581:588	arg1	specificity					615:625	putative glycosyltransferase (GT) specificity	581:625	putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans	581:686	Our model leverages putative glycosyltransferase (GT) specificity to define the biosynthetic pathways for all measured glycans, and the Markov chain modelling is used to learn glycosyltransferase isoform activities and predict glycosylation following glycosyltransferase knock-in/knockout.					
32285041	1	68	theme	pharmaceutical	182:195	arg1	market					197:202	the global pharmaceutical market	171:202	the global pharmaceutical market	171:202	Glycosylated biopharmaceuticals are important in the global pharmaceutical market.					
33720133	9	0	theme	accumulation	1330:1341	arg1	level					1321:1325	their level	1315:1325	their level of accumulation in the cell wall	1315:1358	This technique allows subcellular localization of glycans and detects their level of accumulation in the cell wall.					
33720133	4	1	theme	fluorescent	483:493	arg1	immunolocalization					495:512	The fluorescent immunolocalization	479:512	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections	479:571	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	7	2	theme	samples	1017:1023	arg1	sections					992:999	Thin sections	987:999	Thin sections of the embedded samples obtained with an ultra-microtome	987:1056	Thin sections of the embedded samples obtained with an ultra-microtome were used for immunolocalization with specific antibodies.					
33720133	1	3	theme	cell	161:164	arg1	wall					166:169	the cell wall composition and structure	157:195	the cell wall composition and structure	157:195	Plant development involves constant adjustments of the cell wall composition and structure in response to both internal and external stimuli.					
33720133	0	4	from	Immunolocalization	12:29	arg1	Wall					83:86	the Cell Wall	74:86	the Cell Wall of Plant Tissues	74:103	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.					
33720133	1	5	theme	wall	166:169	arg1	adjustments					142:152	constant adjustments	133:152	constant adjustments of the cell wall composition and structure	133:195	Plant development involves constant adjustments of the cell wall composition and structure in response to both internal and external stimuli.					
33720133	4	6	theme	key	583:585	arg1	tool					587:590	a key tool	581:590	a key tool to uncover remodeling of wall polysaccharide networks, structure and components	581:670	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	0	7	theme	Tissues	97:103	arg1	Wall					83:86	the Cell Wall	74:86	the Cell Wall of Plant Tissues	74:103	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.					
33720133	9	8	theme	cell	1350:1353	arg1	wall					1355:1358	the cell wall	1346:1358	the cell wall	1346:1358	This technique allows subcellular localization of glycans and detects their level of accumulation in the cell wall.					
33720133	4	9	from	immunolocalization	495:512	arg1	sections					564:571	plant histological sections	545:571	plant histological sections	545:571	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	1	10	theme	constant	133:140	arg1	adjustments					142:152	constant adjustments	133:152	constant adjustments of the cell wall composition and structure	133:195	Plant development involves constant adjustments of the cell wall composition and structure in response to both internal and external stimuli.					
33720133	2	11	theme	non-cellulosic	289:302	arg1	polysaccharides					304:318	cellulose and non-cellulosic polysaccharides	275:318	cellulose and non-cellulosic polysaccharides together with proteins, phenolic compounds and water	275:371	Cell walls are composed of cellulose and non-cellulosic polysaccharides together with proteins, phenolic compounds and water.					
33720133	10	12	theme	spatio-temporal	1398:1412	arg1	patterns					1414:1421	spatio-temporal patterns	1398:1421	spatio-temporal patterns of AGP and pectin distribution	1398:1452	It also permits the determination of spatio-temporal patterns of AGP and pectin distribution during developmental processes.					
33720133	0	13	theme	Plant	91:95	arg1	Tissues					97:103	Plant Tissues	91:103	Plant Tissues	91:103	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.					
33720133	6	14	theme	better	926:931	arg1	preservation					933:944	a better preservation	924:944	a better preservation of the tissue structure and composition	924:984	Paraformaldehyde/glutaraldehyde fixation was used along with LR-White embedding of the plant samples, allowing for a better preservation of the tissue structure and composition.					
33720133	7	15	theme	Thin	987:990	arg1	sections					992:999	Thin sections	987:999	Thin sections of the embedded samples obtained with an ultra-microtome	987:1056	Thin sections of the embedded samples obtained with an ultra-microtome were used for immunolocalization with specific antibodies.					
33720133	2	16	theme	cellulose	275:283	arg1	polysaccharides					304:318	cellulose and non-cellulosic polysaccharides	275:318	cellulose and non-cellulosic polysaccharides together with proteins, phenolic compounds and water	275:371	Cell walls are composed of cellulose and non-cellulosic polysaccharides together with proteins, phenolic compounds and water.					
33720133	11	17	theme	tool	1502:1505	arg1	use					1490:1492	The use	1486:1492	The use of this tool	1486:1505	The use of this tool may ultimately guide research directions and link glycans to specific functions in plants.					
33720133	6	18	theme	Paraformaldehyde/glutaraldehyde	809:839	arg1	fixation					841:848	Paraformaldehyde/glutaraldehyde fixation	809:848	Paraformaldehyde/glutaraldehyde fixation	809:848	Paraformaldehyde/glutaraldehyde fixation was used along with LR-White embedding of the plant samples, allowing for a better preservation of the tissue structure and composition.					
33720133	0	19	theme	Fluorescent	0:10	arg1	Immunolocalization					12:29	Fluorescent Immunolocalization	0:29	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.	0:104	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.					
33720133	9	20	theme	glycans	1295:1301	arg1	localization					1279:1290	subcellular localization	1267:1290	subcellular localization of glycans	1267:1301	This technique allows subcellular localization of glycans and detects their level of accumulation in the cell wall.					
33720133	7	21	with	immunolocalization	1072:1089	arg1	antibodies					1105:1114	specific antibodies	1096:1114	specific antibodies	1096:1114	Thin sections of the embedded samples obtained with an ultra-microtome were used for immunolocalization with specific antibodies.					
33720133	3	22	theme	cell	385:388	arg1	wall					390:393	the cell wall	381:393	the cell wall	381:393	90% of the cell wall is composed of polysaccharides (e.g., pectins) and arabinogalactan proteins (AGPs).					
33720133	9	23	from	level	1321:1325	arg1	wall					1355:1358	the cell wall	1346:1358	the cell wall	1346:1358	This technique allows subcellular localization of glycans and detects their level of accumulation in the cell wall.					
33720133	8	24	theme	great	1139:1143	arg1	resolution					1145:1154	great resolution	1139:1154	great resolution	1139:1154	This technique offers great resolution, high specificity, and the chance to detect multiple glycan epitopes in the same sample.					
33720133	3	25	theme	wall	390:393	arg1	wall					390:393	the cell wall	381:393	the cell wall	381:393	90% of the cell wall is composed of polysaccharides (e.g., pectins) and arabinogalactan proteins (AGPs).					
33720133	3	25	theme	wall	390:393	arg1	%					376:376	90%	374:376	90% of the cell wall	374:393	90% of the cell wall is composed of polysaccharides (e.g., pectins) and arabinogalactan proteins (AGPs).					
33720133	8	26	theme	high	1157:1160	arg1	specificity					1162:1172	high specificity	1157:1172	high specificity	1157:1172	This technique offers great resolution, high specificity, and the chance to detect multiple glycan epitopes in the same sample.					
33720133	1	27	dep	wall	166:169	arg1	structure					187:195	structure	187:195	structure	187:195	Plant development involves constant adjustments of the cell wall composition and structure in response to both internal and external stimuli.					
33720133	1	27	dep	wall	166:169	arg1	composition					171:181	composition	171:181	composition	171:181	Plant development involves constant adjustments of the cell wall composition and structure in response to both internal and external stimuli.					
33720133	0	28	theme	Arabinogalactan	34:48	arg1	Proteins					50:57	Arabinogalactan Proteins	34:57	Arabinogalactan Proteins	34:57	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.					
33720133	4	29	theme	histological	551:562	arg1	sections					564:571	plant histological sections	545:571	plant histological sections	545:571	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	5	30	theme	fluorescent	702:712	arg1	procedure					733:741	an optimized fluorescent immunolocalization procedure	689:741	an optimized fluorescent immunolocalization procedure to detect glycan epitopes from AGPs and pectins in plant tissues	689:806	Here, we report an optimized fluorescent immunolocalization procedure to detect glycan epitopes from AGPs and pectins in plant tissues.					
33720133	9	31	theme	subcellular	1267:1277	arg1	localization					1279:1290	subcellular localization	1267:1290	subcellular localization of glycans	1267:1301	This technique allows subcellular localization of glycans and detects their level of accumulation in the cell wall.					
33720133	11	32	theme	research	1528:1535	arg1	directions					1537:1546	research directions	1528:1546	research directions	1528:1546	The use of this tool may ultimately guide research directions and link glycans to specific functions in plants.					
33720133	11	33	from	functions	1577:1585	arg1	plants					1590:1595	plants	1590:1595	plants	1590:1595	The use of this tool may ultimately guide research directions and link glycans to specific functions in plants.					
33720133	5	34	theme	immunolocalization	714:731	arg1	procedure					733:741	an optimized fluorescent immunolocalization procedure	689:741	an optimized fluorescent immunolocalization procedure to detect glycan epitopes from AGPs and pectins in plant tissues	689:806	Here, we report an optimized fluorescent immunolocalization procedure to detect glycan epitopes from AGPs and pectins in plant tissues.					
33720133	1	35	theme	Plant	106:110	arg1	development					112:122	Plant development	106:122	Plant development	106:122	Plant development involves constant adjustments of the cell wall composition and structure in response to both internal and external stimuli.					
33720133	4	36	theme	networks	637:644	arg1	remodeling					603:612	remodeling	603:612	remodeling of wall polysaccharide networks, structure and components	603:670	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	5	37	theme	plant	794:798	arg1	tissues					800:806	plant tissues	794:806	plant tissues	794:806	Here, we report an optimized fluorescent immunolocalization procedure to detect glycan epitopes from AGPs and pectins in plant tissues.					
33720133	6	38	theme	samples	902:908	arg1	embedding					879:887	LR-White embedding	870:887	LR-White embedding	870:887	Paraformaldehyde/glutaraldehyde fixation was used along with LR-White embedding of the plant samples, allowing for a better preservation of the tissue structure and composition.					
33720133	6	39	used	used	854:857	arg2	fixation					841:848	Paraformaldehyde/glutaraldehyde fixation	809:848	Paraformaldehyde/glutaraldehyde fixation	809:848	Paraformaldehyde/glutaraldehyde fixation was used along with LR-White embedding of the plant samples, allowing for a better preservation of the tissue structure and composition.					
33720133	0	40	theme	Proteins	50:57	arg1	Immunolocalization					12:29	Fluorescent Immunolocalization	0:29	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.	0:104	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.					
33720133	4	41	theme	epitopes	533:540	arg1	immunolocalization					495:512	The fluorescent immunolocalization	479:512	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections	479:571	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	5	42	theme	glycan	753:758	arg1	epitopes					760:767	glycan epitopes	753:767	glycan epitopes	753:767	Here, we report an optimized fluorescent immunolocalization procedure to detect glycan epitopes from AGPs and pectins in plant tissues.					
33720133	4	43	theme	specific	517:524	arg1	epitopes					533:540	specific glycan epitopes	517:540	specific glycan epitopes	517:540	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	1	44	theme	internal	217:224	arg1	stimuli					239:245	both internal and external stimuli	212:245	both internal and external stimuli	212:245	Plant development involves constant adjustments of the cell wall composition and structure in response to both internal and external stimuli.					
33720133	4	45	theme	glycan	526:531	arg1	epitopes					533:540	specific glycan epitopes	517:540	specific glycan epitopes	517:540	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	12	46	theme	expression	1672:1681	arg1	studies					1683:1689	gene expression studies	1667:1689	gene expression studies	1667:1689	Furthermore, the information obtained can complement biochemical and gene expression studies.					
33720133	3	47	dep	pectins	433:439	arg1	e.g.					427:430	e.g.	427:430	e.g.	427:430	90% of the cell wall is composed of polysaccharides (e.g., pectins) and arabinogalactan proteins (AGPs).					
33720133	12	48	theme	gene	1667:1670	arg1	studies					1683:1689	gene expression studies	1667:1689	gene expression studies	1667:1689	Furthermore, the information obtained can complement biochemical and gene expression studies.					
33720133	0	49	theme	Pectins	63:69	arg1	Immunolocalization					12:29	Fluorescent Immunolocalization	0:29	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.	0:104	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.					
33720133	4	50	theme	plant	545:549	arg1	sections					564:571	plant histological sections	545:571	plant histological sections	545:571	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	7	51	theme	specific	1096:1103	arg1	antibodies					1105:1114	specific antibodies	1096:1114	specific antibodies	1096:1114	Thin sections of the embedded samples obtained with an ultra-microtome were used for immunolocalization with specific antibodies.					
33720133	6	52	theme	plant	896:900	arg1	samples					902:908	the plant samples	892:908	the plant samples	892:908	Paraformaldehyde/glutaraldehyde fixation was used along with LR-White embedding of the plant samples, allowing for a better preservation of the tissue structure and composition.					
33720133	11	53	theme	link	1552:1555	arg1	glycans					1557:1563	link glycans	1552:1563	link glycans	1552:1563	The use of this tool may ultimately guide research directions and link glycans to specific functions in plants.					
33720133	6	54	theme	structure	960:968	arg1	preservation					933:944	a better preservation	924:944	a better preservation of the tissue structure and composition	924:984	Paraformaldehyde/glutaraldehyde fixation was used along with LR-White embedding of the plant samples, allowing for a better preservation of the tissue structure and composition.					
33720133	8	55	theme	multiple	1200:1207	arg1	epitopes					1216:1223	multiple glycan epitopes	1200:1223	multiple glycan epitopes	1200:1223	This technique offers great resolution, high specificity, and the chance to detect multiple glycan epitopes in the same sample.					
33720133	10	56	dep	AGP	1426:1428	arg1	distribution					1441:1452	distribution	1441:1452	distribution	1441:1452	It also permits the determination of spatio-temporal patterns of AGP and pectin distribution during developmental processes.					
33720133	2	57	theme	phenolic	344:351	arg1	compounds					353:361	phenolic compounds	344:361	phenolic compounds	344:361	Cell walls are composed of cellulose and non-cellulosic polysaccharides together with proteins, phenolic compounds and water.					
33720133	10	58	theme	patterns	1414:1421	arg1	determination					1381:1393	the determination	1377:1393	the determination of spatio-temporal patterns of AGP and pectin distribution	1377:1452	It also permits the determination of spatio-temporal patterns of AGP and pectin distribution during developmental processes.					
33720133	8	59	theme	glycan	1209:1214	arg1	epitopes					1216:1223	multiple glycan epitopes	1200:1223	multiple glycan epitopes	1200:1223	This technique offers great resolution, high specificity, and the chance to detect multiple glycan epitopes in the same sample.					
33720133	5	60	theme	optimized	692:700	arg1	procedure					733:741	an optimized fluorescent immunolocalization procedure	689:741	an optimized fluorescent immunolocalization procedure to detect glycan epitopes from AGPs and pectins in plant tissues	689:806	Here, we report an optimized fluorescent immunolocalization procedure to detect glycan epitopes from AGPs and pectins in plant tissues.					
33720133	6	61	theme	LR-White	870:877	arg1	embedding					879:887	LR-White embedding	870:887	LR-White embedding	870:887	Paraformaldehyde/glutaraldehyde fixation was used along with LR-White embedding of the plant samples, allowing for a better preservation of the tissue structure and composition.					
33720133	10	62	theme	AGP	1426:1428	arg1	patterns					1414:1421	spatio-temporal patterns	1398:1421	spatio-temporal patterns of AGP and pectin distribution	1398:1452	It also permits the determination of spatio-temporal patterns of AGP and pectin distribution during developmental processes.					
33720133	7	63	used	used	1063:1066	arg2	sections					992:999	Thin sections	987:999	Thin sections of the embedded samples obtained with an ultra-microtome	987:1056	Thin sections of the embedded samples obtained with an ultra-microtome were used for immunolocalization with specific antibodies.					
33720133	1	64	theme	external	230:237	arg1	stimuli					239:245	both internal and external stimuli	212:245	both internal and external stimuli	212:245	Plant development involves constant adjustments of the cell wall composition and structure in response to both internal and external stimuli.					
33720133	0	65	theme	Cell	78:81	arg1	Wall					83:86	the Cell Wall	74:86	the Cell Wall of Plant Tissues	74:103	Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues.					
33720133	3	66	theme	arabinogalactan	446:460	arg1	AGPs					472:475	AGPs	472:475	AGPs	472:475	90% of the cell wall is composed of polysaccharides (e.g., pectins) and arabinogalactan proteins (AGPs).					
33720133	3	66	theme	arabinogalactan	446:460	arg1	proteins					462:469	arabinogalactan proteins	446:469	arabinogalactan proteins (AGPs)	446:476	90% of the cell wall is composed of polysaccharides (e.g., pectins) and arabinogalactan proteins (AGPs).					
33720133	11	67	theme	specific	1568:1575	arg1	functions					1577:1585	specific functions	1568:1585	specific functions in plants	1568:1595	The use of this tool may ultimately guide research directions and link glycans to specific functions in plants.					
33720133	10	68	theme	pectin	1434:1439	arg1	patterns					1414:1421	spatio-temporal patterns	1398:1421	spatio-temporal patterns of AGP and pectin distribution	1398:1452	It also permits the determination of spatio-temporal patterns of AGP and pectin distribution during developmental processes.					
33720133	4	69	theme	polysaccharide	622:635	arg1	structure					647:655	structure	647:655	structure	647:655	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	4	69	theme	polysaccharide	622:635	arg1	networks					637:644	wall polysaccharide networks	617:644	wall polysaccharide networks	617:644	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	4	69	theme	polysaccharide	622:635	arg1	components					661:670	components	661:670	components	661:670	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	10	70	theme	developmental	1461:1473	arg1	processes					1475:1483	developmental processes	1461:1483	developmental processes	1461:1483	It also permits the determination of spatio-temporal patterns of AGP and pectin distribution during developmental processes.					
33720133	6	71	theme	tissue	953:958	arg1	structure					960:968	the tissue structure	949:968	the tissue structure	949:968	Paraformaldehyde/glutaraldehyde fixation was used along with LR-White embedding of the plant samples, allowing for a better preservation of the tissue structure and composition.					
33720133	2	72	theme	Cell	248:251	arg1	walls					253:257	Cell walls	248:257	Cell walls	248:257	Cell walls are composed of cellulose and non-cellulosic polysaccharides together with proteins, phenolic compounds and water.					
33720133	7	73	theme	embedded	1008:1015	arg1	samples					1017:1023	the embedded samples	1004:1023	the embedded samples obtained with an ultra-microtome	1004:1056	Thin sections of the embedded samples obtained with an ultra-microtome were used for immunolocalization with specific antibodies.					
33720133	8	74	theme	same	1232:1235	arg1	sample					1237:1242	the same sample	1228:1242	the same sample	1228:1242	This technique offers great resolution, high specificity, and the chance to detect multiple glycan epitopes in the same sample.					
33720133	6	75	theme	composition	974:984	arg1	preservation					933:944	a better preservation	924:944	a better preservation of the tissue structure and composition	924:984	Paraformaldehyde/glutaraldehyde fixation was used along with LR-White embedding of the plant samples, allowing for a better preservation of the tissue structure and composition.					
33720133	4	76	theme	wall	617:620	arg1	structure					647:655	structure	647:655	structure	647:655	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	4	76	theme	wall	617:620	arg1	networks					637:644	wall polysaccharide networks	617:644	wall polysaccharide networks	617:644	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
33720133	4	76	theme	wall	617:620	arg1	components					661:670	components	661:670	components	661:670	The fluorescent immunolocalization of specific glycan epitopes in plant histological sections remains a key tool to uncover remodeling of wall polysaccharide networks, structure and components.					
32583304	3	0	theme	simple/short	660:671	arg1	O-glycans					673:681	simple/short O-glycans	660:681	simple/short O-glycans	660:681	Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation.					
32583304	7	1	theme	cell	1184:1187	arg1	proliferation					1189:1201	PC-3 cell proliferation	1179:1201	PC-3 cell proliferation	1179:1201	ST3Gal-I silencing studies showed that ST3Gal-I was associated with PC-3 cell proliferation, migration and apoptosis.					
32583304	1	2	theme	functions	229:237	arg1	critical					253:260	critical	253:260	critical	253:260	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	1	2	theme	functions	229:237	arg1	understanding					191:203	an understanding	188:203	an understanding of the compositions and functions of glycans	188:248	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	0	3	theme	prostate	66:73	arg1	cancer					75:80	prostate cancer	66:80	prostate cancer	66:80	The expression and functional analysis of the sialyl-T antigen in prostate cancer.					
32583304	0	4	from	expression	4:13	arg1	cancer					75:80	prostate cancer	66:80	prostate cancer	66:80	The expression and functional analysis of the sialyl-T antigen in prostate cancer.					
32583304	2	5	theme	normal	581:586	arg1	tissue					598:603	normal prostatic tissue	581:603	normal prostatic tissue	581:603	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	6	6	theme	possible	1066:1073	arg1	function					1075:1082	the possible function	1062:1082	the possible function of ST3Gal-I in PC-3 cells	1062:1108	We therefore focused on exploring the possible function of ST3Gal-I in PC-3 cells.					
32583304	3	7	theme	decreased	758:766	arg1	fucosylation					768:779	decreased fucosylation	758:779	decreased fucosylation	758:779	Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation.					
32583304	8	8	theme	cancer	1423:1428	arg1	prognosis					1430:1438	metastatic prostate cancer prognosis	1403:1438	metastatic prostate cancer prognosis	1403:1438	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	1	9	theme	glycans	242:248	arg1	compositions					212:223	compositions	212:223	compositions	212:223	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	1	9	theme	glycans	242:248	arg1	functions					229:237	functions	229:237	functions	229:237	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	4	10	theme	PC-3	864:867	arg1	cells					869:873	PC-3 cells	864:873	PC-3 cells	864:873	Core 1 sialylation was increased dramatically in all CaP cells, and especially in PC-3 cells.					
32583304	3	11	theme	cancer	615:620	arg1	cells					628:632	Prostate cancer (CaP) cells	606:632	Prostate cancer (CaP) cells	606:632	Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation.					
32583304	8	12	theme	ST3Gal-I	1290:1297	arg1	regulation					1276:1285	down regulation	1271:1285	down regulation of ST3Gal-I	1271:1297	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	2	13	theme	RWPE-1	555:560	arg1	cells					562:566	immortalized RWPE-1 cells	542:566	immortalized RWPE-1 cells derived from normal prostatic tissue	542:603	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	8	14	theme	in	1237:1238	arg1	studies					1245:1251	Further in vivo studies	1229:1251	Further in vivo studies	1229:1251	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	5	15	theme	Neu5Acα2-3Galβ1-3GalNAc-	894:917	arg1	expression					880:889	The expression	876:889	The expression of Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product of α2,3-sialyltransferase-I (ST3Gal-I)	876:997	The expression of Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product of α2,3-sialyltransferase-I (ST3Gal-I) was substantially increased.					
32583304	2	16	theme	MALDI-TOF-MS	359:370	arg1	analysis					372:379	MALDI-TOF-MS analysis	359:379	MALDI-TOF-MS analysis	359:379	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	3	17	theme	O-glycan	733:740	arg1	sialylation					742:752	increased O-glycan sialylation	723:752	increased O-glycan sialylation	723:752	Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation.					
32583304	8	18	from	target	1470:1475	arg1	treatment					1516:1524	cancer treatment	1509:1524	cancer treatment	1509:1524	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	2	19	theme	immortalized	542:553	arg1	cells					562:566	immortalized RWPE-1 cells	542:566	immortalized RWPE-1 cells derived from normal prostatic tissue	542:603	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	7	20	theme	silencing	1120:1128	arg1	studies					1130:1136	ST3Gal-I silencing studies	1111:1136	ST3Gal-I silencing studies	1111:1136	ST3Gal-I silencing studies showed that ST3Gal-I was associated with PC-3 cell proliferation, migration and apoptosis.					
32583304	8	21	theme	therapeutic	1481:1491	arg1	intervention					1493:1504	therapeutic intervention	1481:1504	therapeutic intervention	1481:1504	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	2	22	theme	cells	435:439	arg1	profiles					407:414	the O-glycan profiles	394:414	the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue	394:603	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	0	23	from	analysis	30:37	arg1	cancer					75:80	prostate cancer	66:80	prostate cancer	66:80	The expression and functional analysis of the sialyl-T antigen in prostate cancer.					
32583304	6	24	theme	PC-3	1099:1102	arg1	cells					1104:1108	PC-3 cells	1099:1108	PC-3 cells	1099:1108	We therefore focused on exploring the possible function of ST3Gal-I in PC-3 cells.					
32583304	1	25	theme	cancer	165:170	arg1	pathology					172:180	cancer pathology	165:180	cancer pathology	165:180	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	2	26	theme	cancer	428:433	arg1	cells					435:439	prostate cancer cells	419:439	prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue	419:603	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	3	27	theme	O-glycans	673:681	arg1	elevation					647:655	an elevation	644:655	an elevation of simple/short O-glycans	644:681	Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation.					
32583304	4	28	theme	Core	782:785	arg1	sialylation					789:799	Core 1 sialylation	782:799	Core 1 sialylation	782:799	Core 1 sialylation was increased dramatically in all CaP cells, and especially in PC-3 cells.					
32583304	1	29	theme	Aberrant	83:90	arg1	characteristic					120:133	a featured characteristic	109:133	a featured characteristic of cancer	109:143	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	1	29	theme	Aberrant	83:90	arg1	glycosylation					92:104	Aberrant glycosylation	83:104	Aberrant glycosylation	83:104	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	0	30	theme	functional	19:28	arg1	analysis					30:37	functional analysis	19:37	functional analysis	19:37	The expression and functional analysis of the sialyl-T antigen in prostate cancer.					
32583304	3	31	theme	complex	704:710	arg1	O-glycans					712:720	complex O-glycans	704:720	complex O-glycans	704:720	Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation.					
32583304	2	32	theme	prostatic	588:596	arg1	tissue					598:603	normal prostatic tissue	581:603	normal prostatic tissue	581:603	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	6	33	theme	ST3Gal-I	1087:1094	arg1	function					1075:1082	the possible function	1062:1082	the possible function of ST3Gal-I in PC-3 cells	1062:1108	We therefore focused on exploring the possible function of ST3Gal-I in PC-3 cells.					
32583304	8	34	theme	cancer	1509:1514	arg1	treatment					1516:1524	cancer treatment	1509:1524	cancer treatment	1509:1524	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	8	35	theme	tumor	1311:1315	arg1	size					1317:1320	the tumor size	1307:1320	the tumor size in xenograft mouse model	1307:1345	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	1	36	dep	compositions	212:223	arg1	the					208:210	the	208:210	the	208:210	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	8	37	theme	xenograft	1325:1333	arg1	model					1341:1345	xenograft mouse model	1325:1345	xenograft mouse model	1325:1345	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	2	38	theme	O-glycan	398:405	arg1	profiles					407:414	the O-glycan profiles	394:414	the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue	394:603	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	1	39	theme	diagnostic	278:287	arg1	biomarkers					289:298	diagnostic biomarkers	278:298	diagnostic biomarkers	278:298	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	8	40	theme	Further	1229:1235	arg1	studies					1245:1251	Further in vivo studies	1229:1251	Further in vivo studies	1229:1251	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	4	41	gly	sialylation	789:799	arg1	cells					839:843	all CaP cells	831:843	all CaP cells	831:843	Core 1 sialylation was increased dramatically in all CaP cells, and especially in PC-3 cells.					
32583304	4	41	gly	sialylation	789:799	arg1	cells					869:873	PC-3 cells	864:873	PC-3 cells	864:873	Core 1 sialylation was increased dramatically in all CaP cells, and especially in PC-3 cells.					
32583304	2	42	theme	lymph	485:489	arg1	LNCaP					497:501	LNCaP	497:501	LNCaP	497:501	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	2	42	theme	lymph	485:489	arg1	node					491:494	lymph node	485:494	lymph node (LNCaP)	485:502	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	8	43	theme	metastatic	1403:1412	arg1	cancer					1423:1428	metastatic prostate cancer	1403:1428	metastatic prostate cancer prognosis	1403:1438	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	2	44	theme	prostate	419:426	arg1	cells					435:439	prostate cancer cells	419:439	prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue	419:603	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	0	45	theme	sialyl-T	46:53	arg1	antigen					55:61	the sialyl-T antigen	42:61	the sialyl-T antigen	42:61	The expression and functional analysis of the sialyl-T antigen in prostate cancer.					
32583304	4	46	theme	all	831:833	arg1	cells					839:843	all CaP cells	831:843	all CaP cells	831:843	Core 1 sialylation was increased dramatically in all CaP cells, and especially in PC-3 cells.					
32583304	7	47	theme	PC-3	1179:1182	arg1	proliferation					1189:1201	PC-3 cell proliferation	1179:1201	PC-3 cell proliferation	1179:1201	ST3Gal-I silencing studies showed that ST3Gal-I was associated with PC-3 cell proliferation, migration and apoptosis.					
32583304	2	48	attach	derived	568:574	arg2	cells					562:566	immortalized RWPE-1 cells	542:566	immortalized RWPE-1 cells derived from normal prostatic tissue	542:603	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	2	48	attach	derived	568:574	arg1	tissue					598:603	normal prostatic tissue	581:603	normal prostatic tissue	581:603	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	1	49	theme	therapeutic	304:314	arg1	targets					316:322	therapeutic targets	304:322	therapeutic targets	304:322	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	5	50	theme	α2,3-sialyltransferase-I	963:986	arg1	Neu5Acα2-3Galβ1-3GalNAc-					894:917	Neu5Acα2-3Galβ1-3GalNAc-	894:917	Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product of α2,3-sialyltransferase-I (ST3Gal-I)	894:997	The expression of Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product of α2,3-sialyltransferase-I (ST3Gal-I) was substantially increased.					
32583304	5	50	theme	α2,3-sialyltransferase-I	963:986	arg1	product					952:958	the product	948:958	the product of α2,3-sialyltransferase-I (ST3Gal-I)	948:997	The expression of Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product of α2,3-sialyltransferase-I (ST3Gal-I) was substantially increased.					
32583304	8	51	theme	prostate	1414:1421	arg1	cancer					1423:1428	metastatic prostate cancer	1403:1428	metastatic prostate cancer prognosis	1403:1438	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	6	52	from	function	1075:1082	arg1	cells					1104:1108	PC-3 cells	1099:1108	PC-3 cells	1099:1108	We therefore focused on exploring the possible function of ST3Gal-I in PC-3 cells.					
32583304	1	53	theme	featured	111:118	arg1	characteristic					120:133	a featured characteristic	109:133	a featured characteristic of cancer	109:143	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	1	53	theme	featured	111:118	arg1	glycosylation					92:104	Aberrant glycosylation	83:104	Aberrant glycosylation	83:104	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	3	54	theme	Prostate	606:613	arg1	CaP					623:625	CaP	623:625	CaP	623:625	Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation.					
32583304	3	54	theme	Prostate	606:613	arg1	cancer					615:620	Prostate cancer	606:620	Prostate cancer (CaP) cells	606:632	Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation.					
32583304	5	55	theme	sialyl-3T	920:928	arg1	Neu5Acα2-3Galβ1-3GalNAc-					894:917	Neu5Acα2-3Galβ1-3GalNAc-	894:917	Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product of α2,3-sialyltransferase-I (ST3Gal-I)	894:997	The expression of Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product of α2,3-sialyltransferase-I (ST3Gal-I) was substantially increased.					
32583304	5	55	theme	sialyl-3T	920:928	arg1	antigen					930:936	sialyl-3T antigen	920:936	sialyl-3T antigen	920:936	The expression of Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product of α2,3-sialyltransferase-I (ST3Gal-I) was substantially increased.					
32583304	8	56	theme	mouse	1335:1339	arg1	model					1341:1345	xenograft mouse model	1325:1345	xenograft mouse model	1325:1345	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	4	57	theme	CaP	835:837	arg1	cells					839:843	all CaP cells	831:843	all CaP cells	831:843	Core 1 sialylation was increased dramatically in all CaP cells, and especially in PC-3 cells.					
32583304	8	58	theme	down	1271:1274	arg1	regulation					1276:1285	down regulation	1271:1285	down regulation of ST3Gal-I	1271:1297	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	2	59	used	used	354:357	arg2	we					351:352	we	351:352	we	351:352	In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue.					
32583304	3	60	theme	increased	723:731	arg1	sialylation					742:752	increased O-glycan sialylation	723:752	increased O-glycan sialylation	723:752	Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation.					
32583304	8	61	from	size	1317:1320	arg1	model					1341:1345	xenograft mouse model	1325:1345	xenograft mouse model	1325:1345	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	0	62	dep	expression	4:13	arg1	The					0:2	The	0:2	The	0:2	The expression and functional analysis of the sialyl-T antigen in prostate cancer.					
32583304	1	63	theme	cancer	138:143	arg1	characteristic					120:133	a featured characteristic	109:133	a featured characteristic of cancer	109:143	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	1	63	theme	cancer	138:143	arg1	glycosylation					92:104	Aberrant glycosylation	83:104	Aberrant glycosylation	83:104	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	1	64	theme	compositions	212:223	arg1	critical					253:260	critical	253:260	critical	253:260	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	1	64	theme	compositions	212:223	arg1	understanding					191:203	an understanding	188:203	an understanding of the compositions and functions of glycans	188:248	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	0	65	theme	antigen	55:61	arg1	expression					4:13	expression	4:13	expression	4:13	The expression and functional analysis of the sialyl-T antigen in prostate cancer.					
32583304	0	65	theme	antigen	55:61	arg1	analysis					30:37	functional analysis	19:37	functional analysis	19:37	The expression and functional analysis of the sialyl-T antigen in prostate cancer.					
32583304	8	66	dep	in	1237:1238	arg1	vivo					1240:1243	vivo	1240:1243	vivo	1240:1243	Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.					
32583304	1	67	from	role	157:160	arg1	pathology					172:180	cancer pathology	165:180	cancer pathology	165:180	Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer.					
32583304	7	68	theme	ST3Gal-I	1111:1118	arg1	studies					1130:1136	ST3Gal-I silencing studies	1111:1136	ST3Gal-I silencing studies	1111:1136	ST3Gal-I silencing studies showed that ST3Gal-I was associated with PC-3 cell proliferation, migration and apoptosis.					
32583304	3	69	theme	O-glycans	712:720	arg1	reduction					691:699	a reduction	689:699	a reduction of complex O-glycans	689:720	Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation.					
34611825	0	0	theme	Vigna	178:182	arg1	Lectin					155:160	Lactose-Binding Lectin	139:160	Lactose-Binding Lectin from Rice Bean (Vigna umbellata)	139:193	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	0	0	theme	Vigna	178:182	arg1	umbellata					184:192	Vigna umbellata	178:192	Vigna umbellata	178:192	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	10	1	theme	secretary	1391:1399	arg1	protein					1401:1407	acidic, stable, hydrophobic, and secretary protein	1358:1407	acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites	1358:1486	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	8	2	theme	protein	1152:1158	arg1	expression					1160:1169	RbL protein expression	1148:1169	RbL protein expression	1148:1169	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	6	3	theme	RbL	931:933	arg1	No					959:960	RbL gene (GenBank accession No	931:960	RbL gene (GenBank accession No	931:960	Sequence analysis of genomic DNA revealed intronless nature of RbL gene (GenBank accession No.					
34611825	8	4	theme	RbL	987:989	arg1	ORF					991:993	The isolated RbL ORF	974:993	The isolated RbL ORF	974:993	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	4	5	with	similarity	694:703	arg1	lectins					764:770	Vigna angularis lectins	748:770	Vigna angularis lectins	748:770	Blast analysis revealed more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins.					
34611825	4	5	with	similarity	694:703	arg1	aconitifolia					731:742	Vigna aconitifolia	725:742	Vigna aconitifolia	725:742	Blast analysis revealed more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins.					
34611825	9	6	theme	protein	1195:1201	arg1	of ~ 35 kDa m.wt					1214:1229	Recombinant protein (His6-RbL) of ~ 35 kDa m.wt	1183:1229	Recombinant protein (His6-RbL) of ~ 35 kDa m.wt	1183:1229	Recombinant protein (His6-RbL) of ~ 35 kDa m.wt was purified using Ni-NTA affinity chromatography to the extent of 0.26 mg/ml.					
34611825	3	7	theme	843 bp	562:567	arg1	ORF					555:557	RbL ORF	551:557	RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds	551:653	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	3	8	theme	RbL	551:553	arg1	ORF					555:557	RbL ORF	551:557	RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds	551:653	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	8	9	dep	incubation	1100:1109	arg1	confirmed					1138:1146	confirmed	1138:1146	confirmed RbL protein expression in E. coli	1138:1180	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	1	10	gly	glycoproteins	208:220	arg1	glycoproteins					208:220	glycoproteins	208:220	glycoproteins and known for their peculiar carbohydrate-binding activity and their insect-pest-resistant properties	208:322	Lectins are glycoproteins and known for their peculiar carbohydrate-binding activity and their insect-pest-resistant properties.					
34611825	1	10	gly	glycoproteins	208:220	arg1	Lectins					196:202	Lectins	196:202	Lectins	196:202	Lectins are glycoproteins and known for their peculiar carbohydrate-binding activity and their insect-pest-resistant properties.					
34611825	3	11	theme	lectin	599:604	arg1	precursor					606:614	long lectin precursor	594:614	long lectin precursor	594:614	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	12	12	theme	Structural	1592:1601	arg1	analysis					1603:1610	Structural analysis	1592:1610	Structural analysis	1592:1610	Structural analysis revealed dominance of β-pleated sheets and β-turns in RbL protein structure.					
34611825	15	13	theme	inhibition	1939:1948	arg1	assays					1950:1955	Agglutination and agglutination inhibition assays	1907:1955	Agglutination and agglutination inhibition assays	1907:1955	Agglutination and agglutination inhibition assays confirmed the binding specificity of RbL protein with the trypsinized rabbit erythrocytes and with the predicted ligands, respectively.					
34611825	17	14	theme	encoding	2338:2345	arg1	protein					2347:2353	encoding protein	2338:2353	encoding protein	2338:2353	The novel information generated in the study is not mere pre-experimental findings but could also lay foundation for future research on exploring RbL gene and encoding protein for different biomedical and biotechnological applications.					
34611825	0	15	theme	Lactose-Binding	139:153	arg1	Lectin					155:160	Lactose-Binding Lectin	139:160	Lactose-Binding Lectin from Rice Bean (Vigna umbellata)	139:193	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	0	15	theme	Lactose-Binding	139:153	arg1	umbellata					184:192	Vigna umbellata	178:192	Vigna umbellata	178:192	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	3	16	theme	bean	626:629	arg1	seeds					649:653	rice bean (Vigna umbellata) seeds	621:653	rice bean (Vigna umbellata) seeds	621:653	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	10	17	dep	In	1310:1311	arg1	silico					1313:1318	silico	1313:1318	silico	1313:1318	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	13	18	theme	putative	1758:1765	arg1	β-D-galactose					1689:1701	β-D-galactose	1689:1701	β-D-galactose	1689:1701	β-D-galactose, N-acetyl-D-glucosamine, and lactose were predicted as putative ligands for RbL protein.					
34611825	13	18	theme	putative	1758:1765	arg1	N-acetyl-D-glucosamine					1704:1725	N-acetyl-D-glucosamine	1704:1725	N-acetyl-D-glucosamine	1704:1725	β-D-galactose, N-acetyl-D-glucosamine, and lactose were predicted as putative ligands for RbL protein.					
34611825	13	18	theme	putative	1758:1765	arg1	ligands					1767:1773	putative ligands	1758:1773	putative ligands for RbL protein	1758:1789	β-D-galactose, N-acetyl-D-glucosamine, and lactose were predicted as putative ligands for RbL protein.					
34611825	13	18	theme	putative	1758:1765	arg1	lactose					1732:1738	lactose	1732:1738	lactose	1732:1738	β-D-galactose, N-acetyl-D-glucosamine, and lactose were predicted as putative ligands for RbL protein.					
34611825	9	19	theme	Ni-NTA	1250:1255	arg1	chromatography					1266:1279	Ni-NTA affinity chromatography	1250:1279	Ni-NTA affinity chromatography	1250:1279	Recombinant protein (His6-RbL) of ~ 35 kDa m.wt was purified using Ni-NTA affinity chromatography to the extent of 0.26 mg/ml.					
34611825	6	20	theme	accession	949:957	arg1	No					959:960	RbL gene (GenBank accession No	931:960	RbL gene (GenBank accession No	931:960	Sequence analysis of genomic DNA revealed intronless nature of RbL gene (GenBank accession No.					
34611825	11	21	theme	Template-based	1489:1502	arg1	model					1585:1589	good quality model	1572:1589	good quality model	1572:1589	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	11	21	theme	Template-based	1489:1502	arg1	model					1507:1511	Template-based 3D model	1489:1511	Template-based 3D model of RbL	1489:1518	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	14	22	theme	predicted	1872:1880	arg1	ligands					1882:1888	the predicted ligands	1868:1888	the predicted ligands	1868:1888	Hydrogen bonding and hydrophobic forces were the major interactions between the predicted ligands and RbL protein.					
34611825	17	23	theme	biotechnological	2384:2399	arg1	applications					2401:2412	different biomedical and biotechnological applications	2359:2412	applications	2401:2412	The novel information generated in the study is not mere pre-experimental findings but could also lay foundation for future research on exploring RbL gene and encoding protein for different biomedical and biotechnological applications.					
34611825	5	24	theme	Phylogenetic	773:784	arg1	analysis					786:793	Phylogenetic analysis	773:793	Phylogenetic analysis	773:793	Phylogenetic analysis also revealed a close relationship between RbL and other legume lectins.					
34611825	15	25	theme	RbL	1994:1996	arg1	protein					1998:2004	RbL protein	1994:2004	RbL protein	1994:2004	Agglutination and agglutination inhibition assays confirmed the binding specificity of RbL protein with the trypsinized rabbit erythrocytes and with the predicted ligands, respectively.					
34611825	3	26	theme	Vigna	632:636	arg1	seeds					649:653	rice bean (Vigna umbellata) seeds	621:653	rice bean (Vigna umbellata) seeds	621:653	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	10	27	with	protein	1401:1407	arg1	site					1442:1445	one signal peptide cleavage site	1414:1445	one signal peptide cleavage site (A26-A27)	1414:1455	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	10	27	with	protein	1401:1407	arg1	sites					1482:1486	four N-glycosylation sites	1461:1486	four N-glycosylation sites	1461:1486	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	10	27	with	protein	1401:1407	arg1	A26-A27					1448:1454	A26-A27	1448:1454	A26-A27	1448:1454	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	4	28	theme	RbL	708:710	arg1	protein					712:718	RbL protein	708:718	RbL protein	708:718	Blast analysis revealed more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins.					
34611825	11	29	theme	RbL	1516:1518	arg1	model					1585:1589	good quality model	1572:1589	good quality model	1572:1589	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	11	29	theme	RbL	1516:1518	arg1	model					1507:1511	Template-based 3D model	1489:1511	Template-based 3D model of RbL	1489:1518	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	14	30	theme	major	1841:1845	arg1	forces					1825:1830	Hydrogen bonding and hydrophobic forces	1792:1830	Hydrogen bonding and hydrophobic forces	1792:1830	Hydrogen bonding and hydrophobic forces were the major interactions between the predicted ligands and RbL protein.					
34611825	14	30	theme	major	1841:1845	arg1	interactions					1847:1858	the major interactions	1837:1858	the major interactions between the predicted ligands and RbL protein	1837:1904	Hydrogen bonding and hydrophobic forces were the major interactions between the predicted ligands and RbL protein.					
34611825	10	31	theme	cleavage	1433:1440	arg1	site					1442:1445	one signal peptide cleavage site	1414:1445	one signal peptide cleavage site (A26-A27)	1414:1455	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	10	31	theme	cleavage	1433:1440	arg1	A26-A27					1448:1454	A26-A27	1448:1454	A26-A27	1448:1454	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	0	32	theme	Gene	83:86	arg1	Characterization					9:24	Characterization	9:24	Characterization	9:24	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	0	32	theme	Gene	83:86	arg1	Analysis					38:45	Expression Analysis	27:45	Expression Analysis	27:45	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	0	32	theme	Gene	83:86	arg1	Cloning					0:6	Cloning	0:6	Cloning	0:6	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	0	32	theme	Gene	83:86	arg1	Studies					66:72	Agglutination Studies	52:72	Agglutination Studies	52:72	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	16	33	theme	defense	2162:2168	arg1	protein					2170:2176	a plant defense protein	2154:2176	a plant defense protein	2154:2176	Gene ontology analysis functionally annotated RbL protein as a plant defense protein.					
34611825	16	33	theme	defense	2162:2168	arg1	protein					2143:2149	RbL protein	2139:2149	RbL protein	2139:2149	Gene ontology analysis functionally annotated RbL protein as a plant defense protein.					
34611825	17	34	theme	pre-experimental	2236:2251	arg1	findings					2253:2260	pre-experimental findings	2236:2260	pre-experimental findings	2236:2260	The novel information generated in the study is not mere pre-experimental findings but could also lay foundation for future research on exploring RbL gene and encoding protein for different biomedical and biotechnological applications.					
34611825	2	35	theme	insecticidal	436:447	arg1	properties					449:458	insecticidal properties	436:458	insecticidal properties from rice bean	436:473	Earlier we have published our research finding on novel gene encoding Bowman-Birk type protease inhibitor with insecticidal properties from rice bean.					
34611825	4	36	dep	%	692:692	arg1	90					690:691	90	690:691	90	690:691	Blast analysis revealed more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins.					
34611825	12	37	theme	protein	1670:1676	arg1	structure					1678:1686	RbL protein structure	1666:1686	RbL protein structure	1666:1686	Structural analysis revealed dominance of β-pleated sheets and β-turns in RbL protein structure.					
34611825	4	38	theme	Vigna	748:752	arg1	lectins					764:770	Vigna angularis lectins	748:770	Vigna angularis lectins	748:770	Blast analysis revealed more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins.					
34611825	1	39	theme	insect-pest-resistant	291:311	arg1	properties					313:322	their insect-pest-resistant properties	285:322	their insect-pest-resistant properties	285:322	Lectins are glycoproteins and known for their peculiar carbohydrate-binding activity and their insect-pest-resistant properties.					
34611825	12	40	theme	β-pleated	1634:1642	arg1	sheets					1644:1649	β-pleated sheets	1634:1649	β-pleated sheets	1634:1649	Structural analysis revealed dominance of β-pleated sheets and β-turns in RbL protein structure.					
34611825	15	41	theme	predicted	2060:2068	arg1	ligands					2070:2076	the predicted ligands	2056:2076	the predicted ligands	2056:2076	Agglutination and agglutination inhibition assays confirmed the binding specificity of RbL protein with the trypsinized rabbit erythrocytes and with the predicted ligands, respectively.					
34611825	10	42	theme	hydrophobic	1374:1384	arg1	protein					1401:1407	acidic, stable, hydrophobic, and secretary protein	1358:1407	acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites	1358:1486	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	5	43	theme	other	846:850	arg1	lectins					859:865	other legume lectins	846:865	other legume lectins	846:865	Phylogenetic analysis also revealed a close relationship between RbL and other legume lectins.					
34611825	6	44	theme	DNA	897:899	arg1	analysis					877:884	Sequence analysis	868:884	Sequence analysis of genomic DNA	868:899	Sequence analysis of genomic DNA revealed intronless nature of RbL gene (GenBank accession No.					
34611825	16	45	theme	Gene	2093:2096	arg1	analysis					2107:2114	Gene ontology analysis	2093:2114	Gene ontology analysis	2093:2114	Gene ontology analysis functionally annotated RbL protein as a plant defense protein.					
34611825	3	46	theme	first	496:500	arg1	report					502:507	first report	496:507	first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds	496:653	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	0	47	theme	Expression	27:36	arg1	Analysis					38:45	Expression Analysis	27:45	Expression Analysis	27:45	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	8	48	theme	0.5 mM	1078:1083	arg1	IPTG					1085:1088	0.5 mM IPTG	1078:1088	0.5 mM IPTG	1078:1088	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	0	49	theme	Agglutination	52:64	arg1	Studies					66:72	Agglutination Studies	52:72	Agglutination Studies	52:72	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	3	50	from	report	502:507	arg1	sequencing					521:530	sequencing	521:530	sequencing	521:530	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	3	50	from	report	502:507	arg1	expression					537:546	expression	537:546	expression	537:546	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	3	50	from	report	502:507	arg1	cloning					512:518	cloning	512:518	cloning	512:518	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	2	51	theme	novel	375:379	arg1	gene					381:384	novel gene	375:384	novel gene encoding Bowman-Birk type protease inhibitor with insecticidal properties from rice bean	375:473	Earlier we have published our research finding on novel gene encoding Bowman-Birk type protease inhibitor with insecticidal properties from rice bean.					
34611825	14	52	theme	Hydrogen	1792:1799	arg1	forces					1825:1830	Hydrogen bonding and hydrophobic forces	1792:1830	Hydrogen bonding and hydrophobic forces	1792:1830	Hydrogen bonding and hydrophobic forces were the major interactions between the predicted ligands and RbL protein.					
34611825	14	52	theme	Hydrogen	1792:1799	arg1	interactions					1847:1858	the major interactions	1837:1858	the major interactions between the predicted ligands and RbL protein	1837:1904	Hydrogen bonding and hydrophobic forces were the major interactions between the predicted ligands and RbL protein.					
34611825	6	53	theme	Sequence	868:875	arg1	analysis					877:884	Sequence analysis	868:884	Sequence analysis of genomic DNA	868:899	Sequence analysis of genomic DNA revealed intronless nature of RbL gene (GenBank accession No.					
34611825	15	54	theme	trypsinized	2015:2025	arg1	erythrocytes					2034:2045	the trypsinized rabbit erythrocytes	2011:2045	the trypsinized rabbit erythrocytes	2011:2045	Agglutination and agglutination inhibition assays confirmed the binding specificity of RbL protein with the trypsinized rabbit erythrocytes and with the predicted ligands, respectively.					
34611825	10	55	theme	signal	1418:1423	arg1	site					1442:1445	one signal peptide cleavage site	1414:1445	one signal peptide cleavage site (A26-A27)	1414:1455	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	10	55	theme	signal	1418:1423	arg1	A26-A27					1448:1454	A26-A27	1448:1454	A26-A27	1448:1454	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	1	56	dep	glycoproteins	208:220	arg1	known					226:230	known	226:230	known for their peculiar carbohydrate-binding activity and their insect-pest-resistant properties	226:322	Lectins are glycoproteins and known for their peculiar carbohydrate-binding activity and their insect-pest-resistant properties.					
34611825	11	57	theme	quality	1577:1583	arg1	model					1585:1589	good quality model	1572:1589	good quality model	1572:1589	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	11	57	theme	quality	1577:1583	arg1	model					1507:1511	Template-based 3D model	1489:1511	Template-based 3D model of RbL	1489:1518	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	10	58	theme	stable	1366:1371	arg1	protein					1401:1407	acidic, stable, hydrophobic, and secretary protein	1358:1407	acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites	1358:1486	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	2	59	theme	type	407:410	arg1	inhibitor					421:429	Bowman-Birk type protease inhibitor	395:429	Bowman-Birk type protease inhibitor with insecticidal properties from rice bean	395:473	Earlier we have published our research finding on novel gene encoding Bowman-Birk type protease inhibitor with insecticidal properties from rice bean.					
34611825	8	60	theme	37 °C.	1114:1119	arg1	blotting					1129:1136	37 °C. Western blotting	1114:1136	37 °C. Western blotting	1114:1136	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	6	61	theme	GenBank	941:947	arg1	No					959:960	RbL gene (GenBank accession No	931:960	RbL gene (GenBank accession No	931:960	Sequence analysis of genomic DNA revealed intronless nature of RbL gene (GenBank accession No.					
34611825	8	62	theme	isolated	978:985	arg1	ORF					991:993	The isolated RbL ORF	974:993	The isolated RbL ORF	974:993	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	10	63	theme	acidic	1358:1363	arg1	protein					1401:1407	acidic, stable, hydrophobic, and secretary protein	1358:1407	acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites	1358:1486	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	1	64	theme	carbohydrate-binding	251:270	arg1	activity					272:279	their peculiar carbohydrate-binding activity	236:279	their peculiar carbohydrate-binding activity	236:279	Lectins are glycoproteins and known for their peculiar carbohydrate-binding activity and their insect-pest-resistant properties.					
34611825	6	65	theme	gene	935:938	arg1	No					959:960	RbL gene (GenBank accession No	931:960	RbL gene (GenBank accession No	931:960	Sequence analysis of genomic DNA revealed intronless nature of RbL gene (GenBank accession No.					
34611825	0	66	from	Bean	172:175	arg1	N-Acetyl-D-Glucosamine					112:133	N-Acetyl-D-Glucosamine	112:133	N-Acetyl-D-Glucosamine	112:133	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	0	66	from	Bean	172:175	arg1	Lectin					155:160	Lactose-Binding Lectin	139:160	Lactose-Binding Lectin from Rice Bean (Vigna umbellata)	139:193	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	0	66	from	Bean	172:175	arg1	β-D-Galactose					97:109	β-D-Galactose	97:109	β-D-Galactose	97:109	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	0	66	from	Bean	172:175	arg1	umbellata					184:192	Vigna umbellata	178:192	Vigna umbellata	178:192	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	11	67	theme	MODELLER	1541:1548	arg1	tool					1550:1553	MODELLER tool	1541:1553	MODELLER tool	1541:1553	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	8	68	theme	RbL	1148:1150	arg1	expression					1160:1169	RbL protein expression	1148:1169	RbL protein expression	1148:1169	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	9	69	theme	Recombinant	1183:1193	arg1	His6-RbL					1204:1211	His6-RbL	1204:1211	His6-RbL	1204:1211	Recombinant protein (His6-RbL) of ~ 35 kDa m.wt was purified using Ni-NTA affinity chromatography to the extent of 0.26 mg/ml.					
34611825	9	69	theme	Recombinant	1183:1193	arg1	protein					1195:1201	Recombinant protein	1183:1201	Recombinant protein (His6-RbL) of ~ 35 kDa m.wt	1183:1229	Recombinant protein (His6-RbL) of ~ 35 kDa m.wt was purified using Ni-NTA affinity chromatography to the extent of 0.26 mg/ml.					
34611825	3	70	dep	precursor	606:614	arg1	seeds					649:653	rice bean (Vigna umbellata) seeds	621:653	rice bean (Vigna umbellata) seeds	621:653	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	6	71	theme	intronless	910:919	arg1	nature					921:926	intronless nature	910:926	intronless nature of RbL gene (GenBank accession No	910:960	Sequence analysis of genomic DNA revealed intronless nature of RbL gene (GenBank accession No.					
34611825	8	72	theme	E.	1012:1013	arg1	cells					1031:1035	E. coli BL-21(DE3) cells	1012:1035	E. coli BL-21(DE3) cells	1012:1035	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	12	73	from	β-turns	1655:1661	arg1	structure					1678:1686	RbL protein structure	1666:1686	RbL protein structure	1666:1686	Structural analysis revealed dominance of β-pleated sheets and β-turns in RbL protein structure.					
34611825	3	74	theme	long	594:597	arg1	precursor					606:614	long lectin precursor	594:614	long lectin precursor	594:614	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	16	75	theme	ontology	2098:2105	arg1	analysis					2107:2114	Gene ontology analysis	2093:2114	Gene ontology analysis	2093:2114	Gene ontology analysis functionally annotated RbL protein as a plant defense protein.					
34611825	8	76	theme	BL-21	1020:1024	arg1	cells					1031:1035	E. coli BL-21(DE3) cells	1012:1035	E. coli BL-21(DE3) cells	1012:1035	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	13	77	theme	RbL	1779:1781	arg1	protein					1783:1789	RbL protein	1779:1789	RbL protein	1779:1789	β-D-galactose, N-acetyl-D-glucosamine, and lactose were predicted as putative ligands for RbL protein.					
34611825	3	78	theme	amino	582:586	arg1	acids					588:592	280 amino acids	578:592	280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds	578:653	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	5	79	theme	legume	852:857	arg1	lectins					859:865	other legume lectins	846:865	other legume lectins	846:865	Phylogenetic analysis also revealed a close relationship between RbL and other legume lectins.					
34611825	11	80	theme	good	1572:1575	arg1	model					1585:1589	good quality model	1572:1589	good quality model	1572:1589	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	11	80	theme	good	1572:1575	arg1	model					1507:1511	Template-based 3D model	1489:1511	Template-based 3D model of RbL	1489:1518	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	10	81	gly	N-glycosylation	1466:1480	arg2	four					1461:1464	four	1461:1464	four	1461:1464	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	10	81	gly	N-glycosylation	1466:1480	arg2	sites					1482:1486	four N-glycosylation sites	1461:1486	four N-glycosylation sites	1461:1486	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	15	82	theme	Agglutination	1907:1919	arg1	assays					1950:1955	Agglutination and agglutination inhibition assays	1907:1955	Agglutination and agglutination inhibition assays	1907:1955	Agglutination and agglutination inhibition assays confirmed the binding specificity of RbL protein with the trypsinized rabbit erythrocytes and with the predicted ligands, respectively.					
34611825	3	83	theme	rice	621:624	arg1	seeds					649:653	rice bean (Vigna umbellata) seeds	621:653	rice bean (Vigna umbellata) seeds	621:653	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	15	84	theme	agglutination	1925:1937	arg1	assays					1950:1955	Agglutination and agglutination inhibition assays	1907:1955	Agglutination and agglutination inhibition assays	1907:1955	Agglutination and agglutination inhibition assays confirmed the binding specificity of RbL protein with the trypsinized rabbit erythrocytes and with the predicted ligands, respectively.					
34611825	4	85	theme	Blast	656:660	arg1	analysis					662:669	Blast analysis	656:669	Blast analysis	656:669	Blast analysis revealed more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins.					
34611825	14	86	theme	RbL	1894:1896	arg1	protein					1898:1904	RbL protein	1894:1904	RbL protein	1894:1904	Hydrogen bonding and hydrophobic forces were the major interactions between the predicted ligands and RbL protein.					
34611825	6	87	theme	No	959:960	arg1	nature					921:926	intronless nature	910:926	intronless nature of RbL gene (GenBank accession No	910:960	Sequence analysis of genomic DNA revealed intronless nature of RbL gene (GenBank accession No.					
34611825	4	88	theme	protein	712:718	arg1	similarity					694:703	more than 90% similarity	680:703	more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins	680:770	Blast analysis revealed more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins.					
34611825	9	89	theme	affinity	1257:1264	arg1	chromatography					1266:1279	Ni-NTA affinity chromatography	1250:1279	Ni-NTA affinity chromatography	1250:1279	Recombinant protein (His6-RbL) of ~ 35 kDa m.wt was purified using Ni-NTA affinity chromatography to the extent of 0.26 mg/ml.					
34611825	3	90	theme	umbellata	638:646	arg1	seeds					649:653	rice bean (Vigna umbellata) seeds	621:653	rice bean (Vigna umbellata) seeds	621:653	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	4	91	theme	Vigna	725:729	arg1	aconitifolia					731:742	Vigna aconitifolia	725:742	Vigna aconitifolia	725:742	Blast analysis revealed more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins.					
34611825	0	92	theme	Novel	77:81	arg1	Gene					83:86	Novel Gene	77:86	Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata)	77:193	Cloning, Characterization, Expression Analysis, and Agglutination Studies of Novel Gene Encoding β-D-Galactose, N-Acetyl-D-Glucosamine and Lactose-Binding Lectin from Rice Bean (Vigna umbellata).					
34611825	8	93	theme	DE3	1026:1028	arg1	cells					1031:1035	E. coli BL-21(DE3) cells	1012:1035	E. coli BL-21(DE3) cells	1012:1035	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	17	94	theme	future	2296:2301	arg1	research					2303:2310	future research	2296:2310	future research on exploring RbL gene and encoding protein for different biomedical and biotechnological applications	2296:2412	The novel information generated in the study is not mere pre-experimental findings but could also lay foundation for future research on exploring RbL gene and encoding protein for different biomedical and biotechnological applications.					
34611825	16	95	theme	RbL	2139:2141	arg1	protein					2170:2176	a plant defense protein	2154:2176	a plant defense protein	2154:2176	Gene ontology analysis functionally annotated RbL protein as a plant defense protein.					
34611825	16	95	theme	RbL	2139:2141	arg1	protein					2143:2149	RbL protein	2139:2149	RbL protein	2139:2149	Gene ontology analysis functionally annotated RbL protein as a plant defense protein.					
34611825	15	96	theme	protein	1998:2004	arg1	specificity					1979:1989	the binding specificity	1967:1989	the binding specificity of RbL protein	1967:2004	Agglutination and agglutination inhibition assays confirmed the binding specificity of RbL protein with the trypsinized rabbit erythrocytes and with the predicted ligands, respectively.					
34611825	11	97	theme	3D	1504:1505	arg1	model					1585:1589	good quality model	1572:1589	good quality model	1572:1589	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	11	97	theme	3D	1504:1505	arg1	model					1507:1511	Template-based 3D model	1489:1511	Template-based 3D model of RbL	1489:1518	Template-based 3D model of RbL was structured using MODELLER tool and validated as good quality model.					
34611825	9	98	theme	0.26 mg/ml	1298:1307	arg1	extent					1288:1293	the extent	1284:1293	the extent of 0.26 mg/ml	1284:1307	Recombinant protein (His6-RbL) of ~ 35 kDa m.wt was purified using Ni-NTA affinity chromatography to the extent of 0.26 mg/ml.					
34611825	10	99	theme	peptide	1425:1431	arg1	site					1442:1445	one signal peptide cleavage site	1414:1445	one signal peptide cleavage site (A26-A27)	1414:1455	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	10	99	theme	peptide	1425:1431	arg1	A26-A27					1448:1454	A26-A27	1448:1454	A26-A27	1448:1454	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	8	100	theme	maximum	1041:1047	arg1	expression					1049:1058	maximum expression	1041:1058	maximum expression	1041:1058	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	4	101	dep	similarity	694:703	arg1	%					692:692	%	692:692	%	692:692	Blast analysis revealed more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins.					
34611825	17	102	theme	RbL	2325:2327	arg1	gene					2329:2332	RbL gene	2325:2332	RbL gene	2325:2332	The novel information generated in the study is not mere pre-experimental findings but could also lay foundation for future research on exploring RbL gene and encoding protein for different biomedical and biotechnological applications.					
34611825	16	103	theme	plant	2156:2160	arg1	protein					2170:2176	a plant defense protein	2154:2176	a plant defense protein	2154:2176	Gene ontology analysis functionally annotated RbL protein as a plant defense protein.					
34611825	16	103	theme	plant	2156:2160	arg1	protein					2143:2149	RbL protein	2139:2149	RbL protein	2139:2149	Gene ontology analysis functionally annotated RbL protein as a plant defense protein.					
34611825	8	104	dep	E.	1012:1013	arg1	coli					1015:1018	coli	1015:1018	coli	1015:1018	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	12	105	theme	RbL	1666:1668	arg1	structure					1678:1686	RbL protein structure	1666:1686	RbL protein structure	1666:1686	Structural analysis revealed dominance of β-pleated sheets and β-turns in RbL protein structure.					
34611825	15	106	theme	binding	1971:1977	arg1	specificity					1979:1989	the binding specificity	1967:1989	the binding specificity of RbL protein	1967:2004	Agglutination and agglutination inhibition assays confirmed the binding specificity of RbL protein with the trypsinized rabbit erythrocytes and with the predicted ligands, respectively.					
34611825	3	107	from	precursor	606:614	arg1	acids					588:592	280 amino acids	578:592	280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds	578:653	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	2	108	theme	protease	412:419	arg1	inhibitor					421:429	Bowman-Birk type protease inhibitor	395:429	Bowman-Birk type protease inhibitor with insecticidal properties from rice bean	395:473	Earlier we have published our research finding on novel gene encoding Bowman-Birk type protease inhibitor with insecticidal properties from rice bean.					
34611825	12	109	from	dominance	1621:1629	arg1	structure					1678:1686	RbL protein structure	1666:1686	RbL protein structure	1666:1686	Structural analysis revealed dominance of β-pleated sheets and β-turns in RbL protein structure.					
34611825	4	110	theme	angularis	754:762	arg1	lectins					764:770	Vigna angularis lectins	748:770	Vigna angularis lectins	748:770	Blast analysis revealed more than 90% similarity of RbL protein with Vigna aconitifolia and Vigna angularis lectins.					
34611825	10	111	theme	In	1310:1311	arg1	analysis					1320:1327	In silico analysis	1310:1327	In silico analysis	1310:1327	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	2	112	theme	rice	465:468	arg1	bean					470:473	rice bean	465:473	rice bean	465:473	Earlier we have published our research finding on novel gene encoding Bowman-Birk type protease inhibitor with insecticidal properties from rice bean.					
34611825	14	113	theme	hydrophobic	1813:1823	arg1	forces					1825:1830	Hydrogen bonding and hydrophobic forces	1792:1830	Hydrogen bonding and hydrophobic forces	1792:1830	Hydrogen bonding and hydrophobic forces were the major interactions between the predicted ligands and RbL protein.					
34611825	14	113	theme	hydrophobic	1813:1823	arg1	interactions					1847:1858	the major interactions	1837:1858	the major interactions between the predicted ligands and RbL protein	1837:1904	Hydrogen bonding and hydrophobic forces were the major interactions between the predicted ligands and RbL protein.					
34611825	6	114	theme	genomic	889:895	arg1	DNA					897:899	genomic DNA	889:899	genomic DNA	889:899	Sequence analysis of genomic DNA revealed intronless nature of RbL gene (GenBank accession No.					
34611825	12	115	theme	sheets	1644:1649	arg1	β-turns					1655:1661	β-turns	1655:1661	β-turns in RbL protein structure	1655:1686	Structural analysis revealed dominance of β-pleated sheets and β-turns in RbL protein structure.					
34611825	12	115	theme	sheets	1644:1649	arg1	dominance					1621:1629	dominance	1621:1629	dominance of β-pleated sheets	1621:1649	Structural analysis revealed dominance of β-pleated sheets and β-turns in RbL protein structure.					
34611825	10	116	theme	N-glycosylation	1466:1480	arg1	sites					1482:1486	four N-glycosylation sites	1461:1486	four N-glycosylation sites	1461:1486	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	17	117	theme	novel	2183:2187	arg1	information					2189:2199	The novel information	2179:2199	The novel information generated in the study	2179:2222	The novel information generated in the study is not mere pre-experimental findings but could also lay foundation for future research on exploring RbL gene and encoding protein for different biomedical and biotechnological applications.					
34611825	5	118	theme	close	811:815	arg1	relationship					817:828	a close relationship	809:828	a close relationship between RbL and other legume lectins	809:865	Phylogenetic analysis also revealed a close relationship between RbL and other legume lectins.					
34611825	14	119	theme	bonding	1801:1807	arg1	forces					1825:1830	Hydrogen bonding and hydrophobic forces	1792:1830	Hydrogen bonding and hydrophobic forces	1792:1830	Hydrogen bonding and hydrophobic forces were the major interactions between the predicted ligands and RbL protein.					
34611825	14	119	theme	bonding	1801:1807	arg1	interactions					1847:1858	the major interactions	1837:1858	the major interactions between the predicted ligands and RbL protein	1837:1904	Hydrogen bonding and hydrophobic forces were the major interactions between the predicted ligands and RbL protein.					
34611825	8	120	theme	4 h	1096:1098	arg1	incubation					1100:1109	4 h incubation	1096:1109	4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli	1096:1180	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	2	121	theme	Bowman-Birk	395:405	arg1	inhibitor					421:429	Bowman-Birk type protease inhibitor	395:429	Bowman-Birk type protease inhibitor with insecticidal properties from rice bean	395:473	Earlier we have published our research finding on novel gene encoding Bowman-Birk type protease inhibitor with insecticidal properties from rice bean.					
34611825	2	122	with	inhibitor	421:429	arg1	properties					449:458	insecticidal properties	436:458	insecticidal properties from rice bean	436:473	Earlier we have published our research finding on novel gene encoding Bowman-Birk type protease inhibitor with insecticidal properties from rice bean.					
34611825	10	123	theme	RbL	1343:1345	arg1	protein					1347:1353	RbL protein	1343:1353	RbL protein	1343:1353	In silico analysis characterized RbL protein as acidic, stable, hydrophobic, and secretary protein with one signal peptide cleavage site (A26-A27) and four N-glycosylation sites.					
34611825	2	124	from	bean	470:473	arg1	properties					449:458	insecticidal properties	436:458	insecticidal properties from rice bean	436:473	Earlier we have published our research finding on novel gene encoding Bowman-Birk type protease inhibitor with insecticidal properties from rice bean.					
34611825	15	125	theme	rabbit	2027:2032	arg1	erythrocytes					2034:2045	the trypsinized rabbit erythrocytes	2011:2045	the trypsinized rabbit erythrocytes	2011:2045	Agglutination and agglutination inhibition assays confirmed the binding specificity of RbL protein with the trypsinized rabbit erythrocytes and with the predicted ligands, respectively.					
34611825	2	126	theme	research	355:362	arg1	finding					364:370	our research finding	351:370	our research finding	351:370	Earlier we have published our research finding on novel gene encoding Bowman-Birk type protease inhibitor with insecticidal properties from rice bean.					
34611825	8	127	theme	Western	1121:1127	arg1	blotting					1129:1136	37 °C. Western blotting	1114:1136	37 °C. Western blotting	1114:1136	The isolated RbL ORF was expressed in E. coli BL-21(DE3) cells and maximum expression was recorded with 0.5 mM IPTG after 4 h incubation at 37 °C. Western blotting confirmed RbL protein expression in E. coli.					
34611825	3	128	theme	ORF	555:557	arg1	sequencing					521:530	sequencing	521:530	sequencing	521:530	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	3	128	theme	ORF	555:557	arg1	expression					537:546	expression	537:546	expression	537:546	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	3	128	theme	ORF	555:557	arg1	cloning					512:518	cloning	512:518	cloning	512:518	This paper presents first report on cloning, sequencing, and expression of RbL ORF of 843 bp encoding 280 amino acids long lectin precursor from rice bean (Vigna umbellata) seeds.					
34611825	1	129	theme	peculiar	242:249	arg1	activity					272:279	their peculiar carbohydrate-binding activity	236:279	their peculiar carbohydrate-binding activity	236:279	Lectins are glycoproteins and known for their peculiar carbohydrate-binding activity and their insect-pest-resistant properties.					
32653682	3	0	theme	red	278:280	arg1	powder					297:302	red ginger extract powder	278:302	red ginger extract powder	278:302	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	4	1	dep	compositions	508:519	arg1	gum					569:571	gum	569:571	three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5	502:608	This study consisted of three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5.					
32653682	5	2	theme	ginger	740:745	arg1	powder					755:760	red ginger extract powder	736:760	red ginger extract powder	736:760	The results showed that several compositions of encapsulant materials produced different stability of bioactive compounds of red ginger extract powder.					
32653682	0	3	theme	compounds	64:72	arg1	stability					41:49	the stability	37:49	the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe	37:114	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.					
32653682	0	4	from	role	4:7	arg1	stability					41:49	the stability	37:49	the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe	37:114	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.					
32653682	6	5	theme	arabic	815:820	arg1	amount					801:806	increasing amount	790:806	increasing amount of gum arabic used	790:825	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	6	5	theme	arabic	815:820	arg1	arabic					815:820	gum arabic	811:820	gum arabic used	811:825	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	0	6	theme	ginger	81:86	arg1	Roscoe					109:114	red ginger (Zingiber officinale Roscoe	77:114	red ginger (Zingiber officinale Roscoe	77:114	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.					
32653682	3	7	theme	study	178:182	arg1	aims					165:168	The aims	161:168	The aims of this study	161:182	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	4	8	theme	arabic	573:578	arg1	gum					569:571	gum	569:571	three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5	502:608	This study consisted of three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5.					
32653682	0	9	theme	red	77:79	arg1	Roscoe					109:114	red ginger (Zingiber officinale Roscoe	77:114	red ginger (Zingiber officinale Roscoe	77:114	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.					
32653682	5	10	theme	materials	671:679	arg1	compositions					643:654	several compositions	635:654	several compositions of encapsulant materials	635:679	The results showed that several compositions of encapsulant materials produced different stability of bioactive compounds of red ginger extract powder.					
32653682	6	11	theme	study	776:780	arg1	result					782:787	the study result	772:787	the study result	772:787	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	0	12	theme	Zingiber	89:96	arg1	Roscoe					109:114	red ginger (Zingiber officinale Roscoe	77:114	red ginger (Zingiber officinale Roscoe	77:114	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.					
32653682	3	13	theme	extract	404:410	arg1	powder					412:417	red ginger extract powder	393:417	red ginger extract powder	393:417	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	5	14	theme	compounds	723:731	arg1	stability					700:708	different stability	690:708	different stability of bioactive compounds of red ginger extract powder	690:760	The results showed that several compositions of encapsulant materials produced different stability of bioactive compounds of red ginger extract powder.					
32653682	3	15	theme	encapsulant	213:223	arg1	materials					225:233	encapsulant materials	213:233	encapsulant materials	213:233	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	5	16	theme	bioactive	713:721	arg1	compounds					723:731	bioactive compounds	713:731	bioactive compounds of red ginger extract powder	713:760	The results showed that several compositions of encapsulant materials produced different stability of bioactive compounds of red ginger extract powder.					
32653682	3	17	theme	ginger	282:287	arg1	powder					297:302	red ginger extract powder	278:302	red ginger extract powder	278:302	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	0	18	theme	bioactive	54:62	arg1	compounds					64:72	bioactive compounds	54:72	bioactive compounds of red ginger (Zingiber officinale Roscoe	54:114	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.					
32653682	3	19	theme	red	393:395	arg1	powder					412:417	red ginger extract powder	393:417	red ginger extract powder	393:417	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	6	20	theme	bioactive	869:877	arg1	compounds					879:887	bioactive compounds	869:887	bioactive compounds of the powders during storage	869:917	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	4	21	theme	materials	536:544	arg1	compositions					508:519	three compositions	502:519	three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5	502:608	This study consisted of three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5.					
32653682	3	22	theme	ginger	397:402	arg1	powder					412:417	red ginger extract powder	393:417	red ginger extract powder	393:417	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	0	23	theme	encapsulant	12:22	arg1	materials					24:32	encapsulant materials	12:32	encapsulant materials	12:32	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.					
32653682	3	24	from	role	205:208	arg1	stability					242:250	the stability	238:250	the stability of bioactive compounds of red ginger extract powder	238:302	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	0	25	theme	Roscoe	109:114	arg1	compounds					64:72	bioactive compounds	54:72	bioactive compounds of red ginger (Zingiber officinale Roscoe	54:114	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.					
32653682	2	26	dep	extract	130:136	arg1	powder					138:143	powder	138:143	powder	138:143	Rubrum) extract powder during storage.					
32653682	0	27	theme	officinale	98:107	arg1	Roscoe					109:114	red ginger (Zingiber officinale Roscoe	77:114	red ginger (Zingiber officinale Roscoe	77:114	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.					
32653682	6	28	theme	better	831:836	arg1	protection					838:847	better protection	831:847	better protection	831:847	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	6	29	theme	compounds	879:887	arg1	stability					856:864	the stability	852:864	the stability of bioactive compounds of the powders during storage	852:917	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	5	30	theme	powder	755:760	arg1	compounds					723:731	bioactive compounds	713:731	bioactive compounds of red ginger extract powder	713:760	The results showed that several compositions of encapsulant materials produced different stability of bioactive compounds of red ginger extract powder.					
32653682	3	31	theme	materials	225:233	arg1	role					205:208	the role	201:208	the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder	201:302	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	5	32	theme	different	690:698	arg1	stability					700:708	different stability	690:708	different stability of bioactive compounds of red ginger extract powder	690:760	The results showed that several compositions of encapsulant materials produced different stability of bioactive compounds of red ginger extract powder.					
32653682	0	33	theme	materials	24:32	arg1	role					4:7	The role	0:7	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.	0:115	The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe.					
32653682	5	34	theme	extract	747:753	arg1	powder					755:760	red ginger extract powder	736:760	red ginger extract powder	736:760	The results showed that several compositions of encapsulant materials produced different stability of bioactive compounds of red ginger extract powder.					
32653682	3	35	theme	best	424:427	arg1	stability					429:437	best stability	424:437	best stability of bioactive compounds during storage	424:475	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	3	36	theme	encapsulant	356:366	arg1	materials					368:376	the encapsulant materials	352:376	the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage	352:475	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	3	37	theme	compounds	265:273	arg1	stability					242:250	the stability	238:250	the stability of bioactive compounds of red ginger extract powder	238:302	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	4	38	theme	encapsulant	524:534	arg1	materials					536:544	encapsulant materials	524:544	encapsulant materials which are maltodextrin	524:567	This study consisted of three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5.					
32653682	4	38	theme	encapsulant	524:534	arg1	maltodextrin					556:567	maltodextrin	556:567	maltodextrin	556:567	This study consisted of three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5.					
32653682	3	39	theme	materials	368:376	arg1	composition					337:347	the composition	333:347	the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage	333:475	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	6	40	theme	powders	896:902	arg1	compounds					879:887	bioactive compounds	869:887	bioactive compounds of the powders during storage	869:917	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	5	41	theme	several	635:641	arg1	compositions					643:654	several compositions	635:654	several compositions of encapsulant materials	635:679	The results showed that several compositions of encapsulant materials produced different stability of bioactive compounds of red ginger extract powder.					
32653682	6	42	theme	gum	811:813	arg1	arabic					815:820	gum arabic	811:820	gum arabic used	811:825	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	4	43	with	arabic	573:578	arg1	8:2					597:599	8:2	597:599	8:2	597:599	This study consisted of three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5.					
32653682	4	43	with	arabic	573:578	arg1	5:5					606:608	5:5	606:608	5:5	606:608	This study consisted of three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5.					
32653682	4	43	with	arabic	573:578	arg1	10:0					591:594	ratio 10:0	585:594	ratio 10:0	585:594	This study consisted of three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5.					
32653682	5	44	theme	red	736:738	arg1	powder					755:760	red ginger extract powder	736:760	red ginger extract powder	736:760	The results showed that several compositions of encapsulant materials produced different stability of bioactive compounds of red ginger extract powder.					
32653682	3	45	theme	bioactive	442:450	arg1	compounds					452:460	bioactive compounds	442:460	bioactive compounds	442:460	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	4	46	theme	ratio	585:589	arg1	10:0					591:594	ratio 10:0	585:594	ratio 10:0	585:594	This study consisted of three compositions of encapsulant materials which are maltodextrin:gum arabic with ratio 10:0, 8:2, and 5:5.					
32653682	3	47	theme	bioactive	255:263	arg1	compounds					265:273	bioactive compounds	255:273	bioactive compounds of red ginger extract powder	255:302	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	3	48	theme	compounds	452:460	arg1	stability					429:437	best stability	424:437	best stability of bioactive compounds during storage	424:475	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	6	49	contain	had	827:829	arg2	protection					838:847	better protection	831:847	better protection	831:847	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	6	49	contain	had	827:829	arg1	amount					801:806	increasing amount	790:806	increasing amount of gum arabic used	790:825	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	6	49	contain	had	827:829	arg1	arabic					815:820	gum arabic	811:820	gum arabic used	811:825	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	3	50	theme	extract	289:295	arg1	powder					297:302	red ginger extract powder	278:302	red ginger extract powder	278:302	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	6	51	theme	increasing	790:799	arg1	amount					801:806	increasing amount	790:806	increasing amount of gum arabic used	790:825	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	6	51	theme	increasing	790:799	arg1	arabic					815:820	gum arabic	811:820	gum arabic used	811:825	Based on the study result, increasing amount of gum arabic used had better protection to the stability of bioactive compounds of the powders during storage.					
32653682	3	52	theme	powder	297:302	arg1	compounds					265:273	bioactive compounds	255:273	bioactive compounds of red ginger extract powder	255:302	The aims of this study were to evaluate the role of encapsulant materials on the stability of bioactive compounds of red ginger extract powder during storage and determine the composition of the encapsulant materials which produced red ginger extract powder with best stability of bioactive compounds during storage.					
32653682	5	53	theme	encapsulant	659:669	arg1	materials					671:679	encapsulant materials	659:679	encapsulant materials	659:679	The results showed that several compositions of encapsulant materials produced different stability of bioactive compounds of red ginger extract powder.					
32841667	7	0	theme	ecological	1477:1486	arg1	concept					1488:1494	the ecological concept	1473:1494	the ecological concept of the comprehensive utilization of natural herbal resources	1473:1555	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	1	1	theme	comprehensive	247:259	arg1	value					273:277	the comprehensive utilization value	243:277	the comprehensive utilization value of P. americana herbal residue	243:308	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	3	2	theme	carboxymethyl	693:705	arg1	PAP					668:670	PAP	668:670	PAP	668:670	To improve the topical wound dressing property, a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios were prepared and optimized.					
32841667	3	2	theme	carboxymethyl	693:705	arg1	CMC					718:720	CMC	718:720	CMC	718:720	To improve the topical wound dressing property, a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios were prepared and optimized.					
32841667	3	2	theme	carboxymethyl	693:705	arg1	cellulose					707:715	carboxymethyl cellulose	693:715	carboxymethyl cellulose (CMC)	693:721	To improve the topical wound dressing property, a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios were prepared and optimized.					
32841667	6	3	theme	collagen	1256:1263	arg1	closure					1247:1253	wound closure	1241:1253	wound closure	1241:1253	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	6	3	theme	collagen	1256:1263	arg1	deposition					1265:1274	collagen deposition	1256:1274	collagen deposition	1256:1274	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	7	4	theme	diabetic	1448:1455	arg1	wound					1457:1461	diabetic wound	1448:1461	diabetic wound	1448:1461	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	5	5	dep	And	1011:1013	arg1	then					1015:1018	then	1015:1018	then	1015:1018	And then, the good cytocompatibility of hydrogel was corroborated by 3T3 fibroblast proliferation assay.					
32841667	1	6	theme	americana	188:196	arg1	history					165:171	the long medicinal use history	142:171	the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment	142:240	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	6	7	theme	loading	1148:1154	arg1	PAP					1156:1158	the composite hydrogel loading PAP	1125:1158	the composite hydrogel loading PAP	1125:1158	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	1	8	theme	manifold	202:209	arg1	ulcer					211:215	manifold ulcer	202:215	manifold ulcer	202:215	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	3	9	theme	topical	594:600	arg1	property					617:624	the topical wound dressing property	590:624	the topical wound dressing property	590:624	To improve the topical wound dressing property, a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios were prepared and optimized.					
32841667	4	10	theme	good	874:877	arg1	structures					905:914	good three-dimensional network structures	874:914	good three-dimensional network structures	874:914	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	3	11	theme	different	728:736	arg1	ratios					738:743	different ratios	728:743	different ratios	728:743	To improve the topical wound dressing property, a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios were prepared and optimized.					
32841667	5	12	theme	good	1025:1028	arg1	cytocompatibility					1030:1046	the good cytocompatibility	1021:1046	the good cytocompatibility of hydrogel	1021:1058	And then, the good cytocompatibility of hydrogel was corroborated by 3T3 fibroblast proliferation assay.					
32841667	7	13	dep	prevention	1420:1429	arg1	the					1416:1418	the	1416:1418	the	1416:1418	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	4	14	theme	network	897:903	arg1	structures					905:914	good three-dimensional network structures	874:914	good three-dimensional network structures	874:914	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	6	15	theme	diabetic	1207:1214	arg1	models					1220:1225	diabetic rat models	1207:1225	diabetic rat models	1207:1225	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	4	16	theme	moderate	961:968	arg1	effect					812:817	the physical crosslinking effect	786:817	the physical crosslinking effect among these polymers	786:838	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	4	16	theme	moderate	961:968	arg1	property					981:988	moderate mechanical property	961:988	moderate mechanical property in rheological test	961:1008	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	0	17	theme	Periplaneta	66:76	arg1	americana					78:86	Periplaneta americana	66:86	Periplaneta americana	66:86	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana herbal residue for diabetic wound healing.					
32841667	3	18	theme	dressing	608:615	arg1	property					617:624	the topical wound dressing property	590:624	the topical wound dressing property	590:624	To improve the topical wound dressing property, a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios were prepared and optimized.					
32841667	1	19	theme	long	146:149	arg1	history					165:171	the long medicinal use history	142:171	the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment	142:240	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	2	20	theme	monosaccharide	534:547	arg1	composition					549:559	monosaccharide composition	534:559	monosaccharide composition	534:559	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	7	21	theme	wound	1457:1461	arg1	prevention					1420:1429	prevention	1420:1429	prevention	1420:1429	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	7	21	theme	wound	1457:1461	arg1	treatment					1435:1443	treatment	1435:1443	treatment	1435:1443	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	1	22	theme	use	161:163	arg1	history					165:171	the long medicinal use history	142:171	the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment	142:240	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	4	23	theme	water	935:939	arg1	capacity					951:958	water retention capacity	935:958	water retention capacity	935:958	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	5	24	theme	fibroblast	1084:1093	arg1	assay					1109:1113	3T3 fibroblast proliferation assay	1080:1113	3T3 fibroblast proliferation assay	1080:1113	And then, the good cytocompatibility of hydrogel was corroborated by 3T3 fibroblast proliferation assay.					
32841667	2	25	theme	previous	487:494	arg1	study					496:500	our previous study	483:500	our previous study	483:500	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	2	26	theme	americana	386:394	arg1	residue					403:409	P. americana herbal residue	383:409	P. americana herbal residue with the potential wound healing effect	383:449	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	0	27	theme	wound	116:120	arg1	healing					122:128	diabetic wound healing	107:128	diabetic wound healing	107:128	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana herbal residue for diabetic wound healing.					
32841667	2	28	theme	PAP	461:463	arg1	faction					465:471	PAP faction	461:471	PAP faction	461:471	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	2	29	theme	wound	430:434	arg1	healing					436:442	the potential wound healing	416:442	the potential wound healing effect	416:449	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	0	30	theme	hydrogel	12:19	arg1	polysaccharides					37:51	A composite hydrogel loading natural polysaccharides	0:51	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana	0:86	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana herbal residue for diabetic wound healing.					
32841667	1	31	theme	americana	285:293	arg1	residue					302:308	P. americana herbal residue	282:308	P. americana herbal residue	282:308	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	7	32	theme	natural	1532:1538	arg1	resources					1547:1555	natural herbal resources	1532:1555	natural herbal resources	1532:1555	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	4	33	theme	rheological	993:1003	arg1	test					1005:1008	rheological test	993:1008	rheological test	993:1008	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	0	34	theme	natural	29:35	arg1	polysaccharides					37:51	A composite hydrogel loading natural polysaccharides	0:51	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana	0:86	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana herbal residue for diabetic wound healing.					
32841667	1	35	theme	residue	302:308	arg1	value					273:277	the comprehensive utilization value	243:277	the comprehensive utilization value of P. americana herbal residue	243:308	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	7	36	theme	utilization	1517:1527	arg1	concept					1488:1494	the ecological concept	1473:1494	the ecological concept of the comprehensive utilization of natural herbal resources	1473:1555	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	3	37	theme	novel	629:633	arg1	hydrogel					645:652	a novel composite hydrogel	627:652	a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios	627:743	To improve the topical wound dressing property, a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios were prepared and optimized.					
32841667	4	38	theme	crosslinking	799:810	arg1	effect					812:817	the physical crosslinking effect	786:817	the physical crosslinking effect among these polymers	786:838	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	4	38	theme	crosslinking	799:810	arg1	property					981:988	moderate mechanical property	961:988	moderate mechanical property in rheological test	961:1008	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	1	39	dep	ulcer	211:215	arg1	treatment					232:240	treatment	232:240	treatment	232:240	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	4	40	from	property	981:988	arg1	test					1005:1008	rheological test	993:1008	rheological test	993:1008	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	2	41	from	residue	403:409	arg1	fraction					369:376	a polysaccharide fraction	352:376	a polysaccharide fraction	352:376	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	2	42	theme	polysaccharide	354:367	arg1	fraction					369:376	a polysaccharide fraction	352:376	a polysaccharide fraction	352:376	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	5	43	dep	corroborated	1064:1075	arg1	And					1011:1013	And	1011:1013	And	1011:1013	And then, the good cytocompatibility of hydrogel was corroborated by 3T3 fibroblast proliferation assay.					
32841667	6	44	theme	M2	1277:1278	arg1	closure					1247:1253	wound closure	1241:1253	wound closure	1241:1253	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	6	44	theme	M2	1277:1278	arg1	macrophages					1280:1290	M2 macrophages polarization and angiogenesis	1277:1320	M2 macrophages polarization and angiogenesis	1277:1320	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	6	44	theme	M2	1277:1278	arg1	angiogenesis					1309:1320	angiogenesis	1309:1320	angiogenesis	1309:1320	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	6	44	theme	M2	1277:1278	arg1	polarization					1292:1303	polarization	1292:1303	polarization	1292:1303	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	1	45	theme	utilization	261:271	arg1	value					273:277	the comprehensive utilization value	243:277	the comprehensive utilization value of P. americana herbal residue	243:308	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	2	46	with	residue	403:409	arg1	effect					444:449	the potential wound healing effect	416:449	the potential wound healing effect	416:449	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	4	47	theme	composite	845:853	arg1	hydrogel					855:862	the composite hydrogel	841:862	the composite hydrogel exhibited good three-dimensional network structures	841:914	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	1	48	theme	history	165:171	arg1	view					134:137	view	134:137	view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment	134:240	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	2	49	dep	structural	519:528	arg1	characterization					561:576	characterization	561:576	characterization	561:576	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	6	50	theme	wound	1241:1245	arg1	closure					1247:1253	wound closure	1241:1253	wound closure	1241:1253	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	6	50	theme	wound	1241:1245	arg1	deposition					1265:1274	collagen deposition	1256:1274	collagen deposition	1256:1274	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	6	50	theme	wound	1241:1245	arg1	macrophages					1280:1290	M2 macrophages polarization and angiogenesis	1277:1320	M2 macrophages polarization and angiogenesis	1277:1320	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	1	51	theme	Periplaneta	176:186	arg1	americana					188:196	Periplaneta americana	176:196	Periplaneta americana	176:196	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	6	52	dep	macrophages	1280:1290	arg1	macrophages					1280:1290	M2 macrophages polarization and angiogenesis	1277:1320	M2 macrophages polarization and angiogenesis	1277:1320	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	6	52	dep	macrophages	1280:1290	arg1	angiogenesis					1309:1320	angiogenesis	1309:1320	angiogenesis	1309:1320	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	6	52	dep	macrophages	1280:1290	arg1	polarization					1292:1303	polarization	1292:1303	polarization	1292:1303	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	0	53	attach	derived	53:59	arg2	polysaccharides					37:51	A composite hydrogel loading natural polysaccharides	0:51	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana	0:86	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana herbal residue for diabetic wound healing.					
32841667	0	53	attach	derived	53:59	arg1	americana					78:86	Periplaneta americana	66:86	Periplaneta americana	66:86	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana herbal residue for diabetic wound healing.					
32841667	6	54	theme	hydrogel	1139:1146	arg1	PAP					1156:1158	the composite hydrogel loading PAP	1125:1158	the composite hydrogel loading PAP	1125:1158	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	4	55	theme	good	917:920	arg1	swelling					922:929	good swelling	917:929	good swelling	917:929	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	7	56	theme	promising	1377:1385	arg1	candidate					1402:1410	an effective and promising wound dressing candidate	1360:1410	candidate	1402:1410	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	5	57	theme	3T3	1080:1082	arg1	assay					1109:1113	3T3 fibroblast proliferation assay	1080:1113	3T3 fibroblast proliferation assay	1080:1113	And then, the good cytocompatibility of hydrogel was corroborated by 3T3 fibroblast proliferation assay.					
32841667	6	58	theme	rat	1216:1218	arg1	models					1220:1225	diabetic rat models	1207:1225	diabetic rat models	1207:1225	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	7	59	theme	wound	1387:1391	arg1	candidate					1402:1410	an effective and promising wound dressing candidate	1360:1410	candidate	1402:1410	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	4	60	theme	three-dimensional	879:895	arg1	structures					905:914	good three-dimensional network structures	874:914	good three-dimensional network structures	874:914	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	5	61	theme	hydrogel	1051:1058	arg1	cytocompatibility					1030:1046	the good cytocompatibility	1021:1046	the good cytocompatibility of hydrogel	1021:1058	And then, the good cytocompatibility of hydrogel was corroborated by 3T3 fibroblast proliferation assay.					
32841667	3	62	theme	wound	602:606	arg1	property					617:624	the topical wound dressing property	590:624	the topical wound dressing property	590:624	To improve the topical wound dressing property, a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios were prepared and optimized.					
32841667	6	63	theme	wound	1190:1194	arg1	healing					1196:1202	wound healing	1190:1202	wound healing in diabetic rat models	1190:1225	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	1	64	theme	skin	220:223	arg1	wounds					225:230	skin wounds	220:230	skin wounds	220:230	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	6	65	from	healing	1196:1202	arg1	models					1220:1225	diabetic rat models	1207:1225	diabetic rat models	1207:1225	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	1	66	theme	medicinal	151:159	arg1	history					165:171	the long medicinal use history	142:171	the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment	142:240	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	4	67	theme	mechanical	970:979	arg1	effect					812:817	the physical crosslinking effect	786:817	the physical crosslinking effect among these polymers	786:838	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	4	67	theme	mechanical	970:979	arg1	property					981:988	moderate mechanical property	961:988	moderate mechanical property in rheological test	961:1008	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	0	68	theme	diabetic	107:114	arg1	healing					122:128	diabetic wound healing	107:128	diabetic wound healing	107:128	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana herbal residue for diabetic wound healing.					
32841667	7	69	theme	dressing	1393:1400	arg1	candidate					1402:1410	an effective and promising wound dressing candidate	1360:1410	candidate	1402:1410	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	7	70	theme	resources	1547:1555	arg1	utilization					1517:1527	the comprehensive utilization	1499:1527	the comprehensive utilization of natural herbal resources	1499:1555	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	5	71	theme	proliferation	1095:1107	arg1	assay					1109:1113	3T3 fibroblast proliferation assay	1080:1113	3T3 fibroblast proliferation assay	1080:1113	And then, the good cytocompatibility of hydrogel was corroborated by 3T3 fibroblast proliferation assay.					
32841667	4	72	theme	retention	941:949	arg1	capacity					951:958	water retention capacity	935:958	water retention capacity	935:958	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	2	73	theme	P.	383:384	arg1	residue					403:409	P. americana herbal residue	383:409	P. americana herbal residue with the potential wound healing effect	383:449	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	0	74	theme	composite	2:10	arg1	polysaccharides					37:51	A composite hydrogel loading natural polysaccharides	0:51	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana	0:86	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana herbal residue for diabetic wound healing.					
32841667	2	75	theme	healing	436:442	arg1	effect					444:449	the potential wound healing effect	416:449	the potential wound healing effect	416:449	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	0	76	theme	loading	21:27	arg1	polysaccharides					37:51	A composite hydrogel loading natural polysaccharides	0:51	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana	0:86	A composite hydrogel loading natural polysaccharides derived from Periplaneta americana herbal residue for diabetic wound healing.					
32841667	1	77	theme	P.	282:283	arg1	residue					302:308	P. americana herbal residue	282:308	P. americana herbal residue	282:308	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	2	78	theme	potential	420:428	arg1	healing					436:442	the potential wound healing	416:442	the potential wound healing effect	416:449	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	3	79	theme	composite	635:643	arg1	hydrogel					645:652	a novel composite hydrogel	627:652	a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios	627:743	To improve the topical wound dressing property, a novel composite hydrogel consisting of PAP, carbomer 940 (CBM), carboxymethyl cellulose (CMC) with different ratios were prepared and optimized.					
32841667	1	80	theme	herbal	295:300	arg1	residue					302:308	P. americana herbal residue	282:308	P. americana herbal residue	282:308	In view of the long medicinal use history of Periplaneta americana for manifold ulcer or skin wounds treatment, the comprehensive utilization value of P. americana herbal residue was evaluated.					
32841667	7	81	theme	herbal	1540:1545	arg1	resources					1547:1555	natural herbal resources	1532:1555	natural herbal resources	1532:1555	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
32841667	2	82	theme	herbal	396:401	arg1	residue					403:409	P. americana herbal residue	383:409	P. americana herbal residue with the potential wound healing effect	383:449	In this study, we isolated a polysaccharide fraction from P. americana herbal residue with the potential wound healing effect, named as PAP faction, based on our previous study and provided the structural and monosaccharide composition characterization.					
32841667	6	83	theme	composite	1129:1137	arg1	PAP					1156:1158	the composite hydrogel loading PAP	1125:1158	the composite hydrogel loading PAP	1125:1158	Finally, the composite hydrogel loading PAP has been proved to accelerate wound healing in diabetic rat models, by promoting wound closure, collagen deposition, M2 macrophages polarization and angiogenesis.					
32841667	4	84	theme	physical	790:797	arg1	effect					812:817	the physical crosslinking effect	786:817	the physical crosslinking effect among these polymers	786:838	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	4	84	theme	physical	790:797	arg1	property					981:988	moderate mechanical property	961:988	moderate mechanical property in rheological test	961:1008	Mediated by the physical crosslinking effect among these polymers, the composite hydrogel exhibited good three-dimensional network structures, good swelling and water retention capacity, moderate mechanical property in rheological test.					
32841667	7	85	theme	comprehensive	1503:1515	arg1	utilization					1517:1527	the comprehensive utilization	1499:1527	the comprehensive utilization of natural herbal resources	1499:1555	In summary, this study would provide an effective and promising wound dressing candidate for the prevention and treatment of diabetic wound, based on the ecological concept of the comprehensive utilization of natural herbal resources.					
34229018	4	0	theme	→	927:927	arg1	→					938:938	acidic →(4HexA1)n →	920:938	acidic →(4HexA1)n → in SCP	920:945	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	6	1	theme	hierarchical	1246:1257	arg1	HCA					1277:1279	HCA	1277:1279	HCA	1277:1279	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	1	theme	hierarchical	1246:1257	arg1	analysis					1267:1274	hierarchical cluster analysis	1246:1274	hierarchical cluster analysis (HCA)	1246:1280	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	5	2	theme	SCP	1177:1179	arg1	visualization					1160:1172	rapid visualization	1154:1172	rapid visualization of SCP and SSP by HILIC-MIM-MS	1154:1203	The resulting low-polymerization compositional fingerprinting (LCF) showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS.					
34229018	4	3	theme	4HexA1	929:934	arg1	→					938:938	acidic →(4HexA1)n →	920:938	acidic →(4HexA1)n → in SCP	920:945	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	4	4	theme	→	1041:1041	arg1	n→					1049:1050	neutral →(4Hex1)n→	1033:1050	neutral →(4Hex1)n→	1033:1050	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	3	5	from	elucidation	731:741	arg1	terms					778:782	terms	778:782	terms of 18O labelling	778:799	In addition, HILIC-MS/MS was employed for elucidation of isomeric glycosidic linkages in terms of 18O labelling.					
34229018	5	6	theme	SSP	1185:1187	arg1	visualization					1160:1172	rapid visualization	1154:1172	rapid visualization of SCP and SSP by HILIC-MIM-MS	1154:1203	The resulting low-polymerization compositional fingerprinting (LCF) showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS.					
34229018	6	7	theme	components	1216:1225	arg1	PCA					1237:1239	PCA	1237:1239	PCA	1237:1239	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	7	theme	components	1216:1225	arg1	analysis					1227:1234	Principal components analysis	1206:1234	Principal components analysis (PCA)	1206:1240	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	8	theme	several	1298:1304	arg1	Q-markers					1310:1318	several key Q-markers	1298:1318	several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP	1298:1408	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	9	theme	SSP	1406:1408	arg1	discrimination					1380:1393	rapid discrimination	1374:1393	rapid discrimination of SCP and SSP	1374:1408	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	1	10	theme	assisted	307:314	arg1	MAMA					327:330	MAMA	327:330	MAMA	327:330	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	10	theme	assisted	307:314	arg1	acid					321:324	assisted mild acid	307:324	microwave assisted mild acid (MAMA) depolymerization	297:348	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	2	11	theme	<	653:653	arg1	5000 Da					655:661	Mw < 5000 Da	650:661	Mw < 5000 Da	650:661	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	2	11	theme	<	653:653	arg1	saccharides					637:647	low-polymerization saccharides	618:647	low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP	618:686	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	6	12	theme	Principal	1206:1214	arg1	PCA					1237:1239	PCA	1237:1239	PCA	1237:1239	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	12	theme	Principal	1206:1214	arg1	analysis					1227:1234	Principal components analysis	1206:1234	Principal components analysis (PCA)	1206:1240	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	4	13	dep	confirmed	879:887	arg1	whereas					948:954	whereas	948:954	whereas	948:954	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	4	14	theme	n	936:936	arg1	→					938:938	acidic →(4HexA1)n →	920:938	acidic →(4HexA1)n → in SCP	920:945	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	4	15	theme	MAMA	806:809	arg1	hydrolysates					811:822	The MAMA hydrolysates	802:822	The MAMA hydrolysates	802:822	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	1	16	theme	hydrophilic	182:192	arg1	HILIC-ESI--MS					269:281	HILIC-ESI--MS	269:281	HILIC-ESI--MS	269:281	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	16	theme	hydrophilic	182:192	arg1	spectrometry					255:266	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry	180:266	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization	180:348	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	7	17	theme	structural	1495:1504	arg1	differences					1506:1516	structural differences	1495:1516	structural differences	1495:1516	This practical study showed that the LCF with PCA and HCA could effectively reflect structural differences and could rapidly achieve discrimination of SCP and SSP.					
34229018	1	18	theme	liquid	206:211	arg1	HILIC-ESI--MS					269:281	HILIC-ESI--MS	269:281	HILIC-ESI--MS	269:281	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	18	theme	liquid	206:211	arg1	spectrometry					255:266	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry	180:266	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization	180:348	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	4	19	theme	4HexA1	979:984	arg1	moiety					989:994	acidic →(4HexA1)n→ moiety	970:994	acidic →(4HexA1)n→ moiety	970:994	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	1	20	dep	proposed	353:360	arg1	study					448:452	a case study	441:452	a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP)	441:535	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	5	21	theme	low-polymerization	1074:1091	arg1	LCF					1123:1125	LCF	1123:1125	LCF	1123:1125	The resulting low-polymerization compositional fingerprinting (LCF) showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS.					
34229018	5	21	theme	low-polymerization	1074:1091	arg1	fingerprinting					1107:1120	The resulting low-polymerization compositional fingerprinting (LCF)	1060:1126	The resulting low-polymerization compositional fingerprinting (LCF)	1060:1126	The resulting low-polymerization compositional fingerprinting (LCF) showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS.					
34229018	1	22	theme	electrospray-mass	237:253	arg1	HILIC-ESI--MS					269:281	HILIC-ESI--MS	269:281	HILIC-ESI--MS	269:281	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	22	theme	electrospray-mass	237:253	arg1	spectrometry					255:266	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry	180:266	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization	180:348	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	4	23	theme	n→	986:987	arg1	moiety					989:994	acidic →(4HexA1)n→ moiety	970:994	acidic →(4HexA1)n→ moiety	970:994	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	0	24	theme	interaction	114:124	arg1	spectrometry					166:177	hydrophilic interaction liquid chromatography-electrospray mass spectrometry	102:177	hydrophilic interaction liquid chromatography-electrospray mass spectrometry	102:177	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.					
34229018	6	25	dep	Q-markers	1310:1318	arg1	827					1346:1348	827	1346:1348	827	1346:1348	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	25	dep	Q-markers	1310:1318	arg1	665					1341:1343	665	1341:1343	665	1341:1343	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	25	dep	Q-markers	1310:1318	arg1	1151					1356:1359	1151	1356:1359	1151	1356:1359	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	25	dep	Q-markers	1310:1318	arg1	735					1365:1367	735	1365:1367	735	1365:1367	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	25	dep	Q-markers	1310:1318	arg1	369					1336:1338	369	1336:1338	369	1336:1338	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	4	26	theme	→	858:858	arg1	moiety					869:874	neutral →(4Hex1)n→ moiety	850:874	neutral →(4Hex1)n→ moiety	850:874	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	0	27	theme	chromatography-electrospray	133:159	arg1	spectrometry					166:177	hydrophilic interaction liquid chromatography-electrospray mass spectrometry	102:177	hydrophilic interaction liquid chromatography-electrospray mass spectrometry	102:177	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.					
34229018	1	28	theme	fruit	457:461	arg1	polysaccharides					463:477	fruit polysaccharides	457:477	fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP)	457:535	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	2	29	theme	low-polymerization	618:635	arg1	5000 Da					655:661	Mw < 5000 Da	650:661	Mw < 5000 Da	650:661	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	2	29	theme	low-polymerization	618:635	arg1	saccharides					637:647	low-polymerization saccharides	618:647	low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP	618:686	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	4	30	theme	4Hex1	860:864	arg1	moiety					869:874	neutral →(4Hex1)n→ moiety	850:874	neutral →(4Hex1)n→ moiety	850:874	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	6	31	dep	m/z	1327:1329	arg1	e.g.					1321:1324	e.g.	1321:1324	e.g.	1321:1324	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	2	32	theme	optimized	542:550	arg1	procedure					568:576	The optimized MAMA hydrolysis procedure	538:576	The optimized MAMA hydrolysis procedure	538:576	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	0	33	theme	Low-polymerization	0:17	arg1	fingerprinting					33:46	Low-polymerization compositional fingerprinting	0:46	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.	0:178	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.					
34229018	1	34	theme	unusual	371:377	arg1	discrimination					379:392	discrimination	379:392	discrimination	379:392	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	35	from	polysaccharides	463:477	arg1	chinensis					493:501	chinensis	493:501	chinensis	493:501	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	35	from	polysaccharides	463:477	arg1	sphenanthera					510:521	sphenanthera	510:521	sphenanthera	510:521	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	5	36	theme	rapid	1154:1158	arg1	visualization					1160:1172	rapid visualization	1154:1172	rapid visualization of SCP and SSP by HILIC-MIM-MS	1154:1203	The resulting low-polymerization compositional fingerprinting (LCF) showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS.					
34229018	3	37	theme	isomeric	746:753	arg1	linkages					766:773	isomeric glycosidic linkages	746:773	isomeric glycosidic linkages	746:773	In addition, HILIC-MS/MS was employed for elucidation of isomeric glycosidic linkages in terms of 18O labelling.					
34229018	7	38	theme	practical	1416:1424	arg1	study					1426:1430	This practical study	1411:1430	This practical study	1411:1430	This practical study showed that the LCF with PCA and HCA could effectively reflect structural differences and could rapidly achieve discrimination of SCP and SSP.					
34229018	2	39	theme	hydrolysis	557:566	arg1	procedure					568:576	The optimized MAMA hydrolysis procedure	538:576	The optimized MAMA hydrolysis procedure	538:576	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	0	40	theme	Schisandra	72:81	arg1	polysaccharides					83:97	Schisandra polysaccharides	72:97	Schisandra polysaccharides	72:97	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.					
34229018	1	41	theme	polysaccharides	424:438	arg1	characterization					398:413	characterization	398:413	characterization	398:413	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	41	theme	polysaccharides	424:438	arg1	discrimination					379:392	discrimination	379:392	discrimination	379:392	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	4	42	theme	n→	866:867	arg1	moiety					869:874	neutral →(4Hex1)n→ moiety	850:874	neutral →(4Hex1)n→ moiety	850:874	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	3	43	theme	linkages	766:773	arg1	elucidation					731:741	elucidation	731:741	elucidation of isomeric glycosidic linkages in terms of 18O labelling	731:799	In addition, HILIC-MS/MS was employed for elucidation of isomeric glycosidic linkages in terms of 18O labelling.					
34229018	6	44	theme	rapid	1374:1378	arg1	discrimination					1380:1393	rapid discrimination	1374:1393	rapid discrimination of SCP and SSP	1374:1408	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	4	45	theme	neutral	1033:1039	arg1	n→					1049:1050	neutral →(4Hex1)n→	1033:1050	neutral →(4Hex1)n→	1033:1050	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	6	46	theme	cluster	1259:1265	arg1	HCA					1277:1279	HCA	1277:1279	HCA	1277:1279	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	46	theme	cluster	1259:1265	arg1	analysis					1267:1274	hierarchical cluster analysis	1246:1274	hierarchical cluster analysis (HCA)	1246:1280	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	5	47	theme	compositional	1093:1105	arg1	LCF					1123:1125	LCF	1123:1125	LCF	1123:1125	The resulting low-polymerization compositional fingerprinting (LCF) showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS.					
34229018	5	47	theme	compositional	1093:1105	arg1	fingerprinting					1107:1120	The resulting low-polymerization compositional fingerprinting (LCF)	1060:1126	The resulting low-polymerization compositional fingerprinting (LCF)	1060:1126	The resulting low-polymerization compositional fingerprinting (LCF) showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS.					
34229018	4	48	theme	acidic	920:925	arg1	→					938:938	acidic →(4HexA1)n →	920:938	acidic →(4HexA1)n → in SCP	920:945	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	3	49	theme	18O	787:789	arg1	labelling					791:799	18O labelling	787:799	18O labelling	787:799	In addition, HILIC-MS/MS was employed for elucidation of isomeric glycosidic linkages in terms of 18O labelling.					
34229018	5	50	from	performance	1139:1149	arg1	visualization					1160:1172	rapid visualization	1154:1172	rapid visualization of SCP and SSP by HILIC-MIM-MS	1154:1203	The resulting low-polymerization compositional fingerprinting (LCF) showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS.					
34229018	6	51	theme	key	1306:1308	arg1	Q-markers					1310:1318	several key Q-markers	1298:1318	several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP	1298:1408	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	1	52	theme	microwave	297:305	arg1	depolymerization					333:348	microwave assisted mild acid (MAMA) depolymerization	297:348	microwave assisted mild acid (MAMA) depolymerization	297:348	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	53	theme	mild	316:319	arg1	MAMA					327:330	MAMA	327:330	MAMA	327:330	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	53	theme	mild	316:319	arg1	acid					321:324	assisted mild acid	307:324	microwave assisted mild acid (MAMA) depolymerization	297:348	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	2	54	theme	Mw	650:651	arg1	5000 Da					655:661	Mw < 5000 Da	650:661	Mw < 5000 Da	650:661	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	2	54	theme	Mw	650:651	arg1	saccharides					637:647	low-polymerization saccharides	618:647	low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP	618:686	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	7	55	with	LCF	1448:1450	arg1	HCA					1465:1467	HCA	1465:1467	HCA	1465:1467	This practical study showed that the LCF with PCA and HCA could effectively reflect structural differences and could rapidly achieve discrimination of SCP and SSP.					
34229018	7	55	with	LCF	1448:1450	arg1	PCA					1457:1459	PCA	1457:1459	PCA	1457:1459	This practical study showed that the LCF with PCA and HCA could effectively reflect structural differences and could rapidly achieve discrimination of SCP and SSP.					
34229018	4	56	from	→	938:938	arg1	SCP					943:945	SCP	943:945	SCP	943:945	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	1	57	theme	interaction	194:204	arg1	HILIC-ESI--MS					269:281	HILIC-ESI--MS	269:281	HILIC-ESI--MS	269:281	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	57	theme	interaction	194:204	arg1	spectrometry					255:266	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry	180:266	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization	180:348	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	0	58	theme	hydrophilic	102:112	arg1	spectrometry					166:177	hydrophilic interaction liquid chromatography-electrospray mass spectrometry	102:177	hydrophilic interaction liquid chromatography-electrospray mass spectrometry	102:177	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.					
34229018	1	59	theme	chromatography-negative	213:235	arg1	HILIC-ESI--MS					269:281	HILIC-ESI--MS	269:281	HILIC-ESI--MS	269:281	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	59	theme	chromatography-negative	213:235	arg1	spectrometry					255:266	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry	180:266	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization	180:348	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	2	60	theme	sample	595:600	arg1	preparations					602:613	sample preparations	595:613	sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP	595:686	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	1	61	from	sphenanthera	510:521	arg1	study					448:452	a case study	441:452	a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP)	441:535	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	0	62	theme	liquid	126:131	arg1	spectrometry					166:177	hydrophilic interaction liquid chromatography-electrospray mass spectrometry	102:177	hydrophilic interaction liquid chromatography-electrospray mass spectrometry	102:177	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.					
34229018	1	63	theme	case	443:446	arg1	study					448:452	a case study	441:452	a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP)	441:535	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	0	64	theme	mass	161:164	arg1	spectrometry					166:177	hydrophilic interaction liquid chromatography-electrospray mass spectrometry	102:177	hydrophilic interaction liquid chromatography-electrospray mass spectrometry	102:177	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.					
34229018	2	65	theme	saccharides	637:647	arg1	preparations					602:613	sample preparations	595:613	sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP	595:686	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	4	66	theme	neutral	850:856	arg1	moiety					869:874	neutral →(4Hex1)n→ moiety	850:874	neutral →(4Hex1)n→ moiety	850:874	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	1	67	from	chinensis	493:501	arg1	study					448:452	a case study	441:452	a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP)	441:535	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	68	theme	polysaccharides	463:477	arg1	study					448:452	a case study	441:452	a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP)	441:535	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	4	69	theme	acidic	970:975	arg1	moiety					989:994	acidic →(4HexA1)n→ moiety	970:994	acidic →(4HexA1)n→ moiety	970:994	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	4	70	theme	→	977:977	arg1	moiety					989:994	acidic →(4HexA1)n→ moiety	970:994	acidic →(4HexA1)n→ moiety	970:994	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	0	71	theme	compositional	19:31	arg1	fingerprinting					33:46	Low-polymerization compositional fingerprinting	0:46	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.	0:178	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.					
34229018	5	72	theme	resulting	1064:1072	arg1	LCF					1123:1125	LCF	1123:1125	LCF	1123:1125	The resulting low-polymerization compositional fingerprinting (LCF) showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS.					
34229018	5	72	theme	resulting	1064:1072	arg1	fingerprinting					1107:1120	The resulting low-polymerization compositional fingerprinting (LCF)	1060:1126	The resulting low-polymerization compositional fingerprinting (LCF)	1060:1126	The resulting low-polymerization compositional fingerprinting (LCF) showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS.					
34229018	6	73	theme	989	1351:1353	arg1	827					1346:1348	827	1346:1348	827	1346:1348	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	73	theme	989	1351:1353	arg1	665					1341:1343	665	1341:1343	665	1341:1343	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	73	theme	989	1351:1353	arg1	1151					1356:1359	1151	1356:1359	1151	1356:1359	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	73	theme	989	1351:1353	arg1	735					1365:1367	735	1365:1367	735	1365:1367	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	6	73	theme	989	1351:1353	arg1	369					1336:1338	369	1336:1338	369	1336:1338	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	7	74	theme	SSP	1570:1572	arg1	discrimination					1544:1557	discrimination	1544:1557	discrimination of SCP and SSP	1544:1572	This practical study showed that the LCF with PCA and HCA could effectively reflect structural differences and could rapidly achieve discrimination of SCP and SSP.					
34229018	1	75	from	study	448:452	arg1	chinensis					493:501	chinensis	493:501	chinensis	493:501	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	75	from	study	448:452	arg1	sphenanthera					510:521	sphenanthera	510:521	sphenanthera	510:521	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	7	76	theme	SCP	1562:1564	arg1	discrimination					1544:1557	discrimination	1544:1557	discrimination of SCP and SSP	1544:1572	This practical study showed that the LCF with PCA and HCA could effectively reflect structural differences and could rapidly achieve discrimination of SCP and SSP.					
34229018	4	77	theme	moiety	989:994	arg1	bigger					1013:1018	bigger	1013:1018	bigger	1013:1018	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	4	77	theme	moiety	989:994	arg1	amount					960:965	the amount	956:965	the amount of acidic →(4HexA1)n→ moiety	956:994	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	4	77	theme	moiety	989:994	arg1	moiety					989:994	acidic →(4HexA1)n→ moiety	970:994	acidic →(4HexA1)n→ moiety	970:994	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	0	78	theme	polysaccharides	83:97	arg1	characterization					52:67	characterization	52:67	characterization of Schisandra polysaccharides	52:97	Low-polymerization compositional fingerprinting for characterization of Schisandra polysaccharides by hydrophilic interaction liquid chromatography-electrospray mass spectrometry.					
34229018	1	79	theme	plant	418:422	arg1	polysaccharides					424:438	plant polysaccharides	418:438	plant polysaccharides	418:438	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	2	80	theme	MAMA	552:555	arg1	procedure					568:576	The optimized MAMA hydrolysis procedure	538:576	The optimized MAMA hydrolysis procedure	538:576	The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (Mw < 5000 Da) released in SCP and SSP.					
34229018	6	81	theme	SCP	1398:1400	arg1	discrimination					1380:1393	rapid discrimination	1374:1393	rapid discrimination of SCP and SSP	1374:1408	Principal components analysis (PCA) and hierarchical cluster analysis (HCA) further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP.					
34229018	4	82	theme	4Hex1	1043:1047	arg1	n→					1049:1050	neutral →(4Hex1)n→	1033:1050	neutral →(4Hex1)n→	1033:1050	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	3	83	theme	glycosidic	755:764	arg1	linkages					766:773	isomeric glycosidic linkages	746:773	isomeric glycosidic linkages	746:773	In addition, HILIC-MS/MS was employed for elucidation of isomeric glycosidic linkages in terms of 18O labelling.					
34229018	1	84	theme	acid	321:324	arg1	depolymerization					333:348	microwave assisted mild acid (MAMA) depolymerization	297:348	microwave assisted mild acid (MAMA) depolymerization	297:348	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	85	dep	chinensis	493:501	arg1	SCP					524:526	SCP	524:526	SCP	524:526	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	1	85	dep	chinensis	493:501	arg1	SSP					532:534	SSP	532:534	SSP	532:534	A hydrophilic interaction liquid chromatography-negative electrospray-mass spectrometry (HILIC-ESI--MS) coupled with microwave assisted mild acid (MAMA) depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in Schisandra chinensis and S. sphenanthera (SCP and SSP).					
34229018	3	86	theme	labelling	791:799	arg1	terms					778:782	terms	778:782	terms of 18O labelling	778:799	In addition, HILIC-MS/MS was employed for elucidation of isomeric glycosidic linkages in terms of 18O labelling.					
34229018	4	87	theme	moiety	869:874	arg1	amount					840:845	the amount	836:845	the amount of neutral →(4Hex1)n→ moiety	836:874	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	4	87	theme	moiety	869:874	arg1	moiety					869:874	neutral →(4Hex1)n→ moiety	850:874	neutral →(4Hex1)n→ moiety	850:874	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
34229018	4	87	theme	moiety	869:874	arg1	bigger					900:905	bigger	900:905	bigger	900:905	The MAMA hydrolysates showed that the amount of neutral →(4Hex1)n→ moiety is confirmed to be more bigger than that of acidic →(4HexA1)n → in SCP, whereas the amount of acidic →(4HexA1)n→ moiety seems to be more bigger than that of neutral →(4Hex1)n→ in SSP.					
32568312	5	0	theme	N-linked	1172:1179	arg1	glycopeptides					1188:1200	the N-linked intact glycopeptides	1168:1200	the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software	1168:1239	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	7	1	theme	BPH	1595:1597	arg1	patients					1599:1606	BPH patients	1595:1606	BPH patients (PCa/BPH ratio = 5.74, p = 0.001)	1595:1640	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	4	2	theme	purified	941:948	arg1	IgGs					950:953	purified IgGs	941:953	purified IgGs from 51 PCa patients and 45 BPH patients	941:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	0	3	link	N-linked	20:27	arg1	signatures					49:58	N-linked intact glycopeptide signatures	20:58	N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	20:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	7	4	theme	PCa	1559:1561	arg1	patients					1563:1570	PCa patients	1559:1570	PCa patients	1559:1570	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	10	5	theme	prostate	2158:2165	arg1	diseases					2167:2174	prostate diseases	2158:2174	prostate diseases	2158:2174	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	3	6	theme	molecular	776:784	arg1	pathogenesis					786:797	molecular pathogenesis	776:797	molecular pathogenesis	776:797	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	6	7	theme	N-linked	1390:1397	arg1	result					1347:1352	a result	1345:1352	a result	1345:1352	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	6	7	theme	N-linked	1390:1397	arg1	glycopeptides					1406:1418	four aberrantly expressed N-linked intact glycopeptides	1364:1418	four aberrantly expressed N-linked intact glycopeptides	1364:1418	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	1	8	theme	prostatic	299:307	arg1	BPH					322:324	BPH	322:324	BPH	322:324	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	1	8	theme	prostatic	299:307	arg1	hyperplasia					309:319	benign prostatic hyperplasia	292:319	benign prostatic hyperplasia (BPH) patients	292:334	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	6	9	link	N-linked	1390:1397	arg1	result					1347:1352	a result	1345:1352	a result	1345:1352	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	6	9	link	N-linked	1390:1397	arg1	glycopeptides					1406:1418	four aberrantly expressed N-linked intact glycopeptides	1364:1418	four aberrantly expressed N-linked intact glycopeptides	1364:1418	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	1	10	theme	novel	197:201	arg1	biomarkers					216:225	novel non-invasive biomarkers	197:225	novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients	197:334	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	9	11	from	BPH	1894:1896	arg1	PCa					1885:1887	PCa	1885:1887	PCa from BPH than the clinical index t-PSA (auROC = 0.681)	1885:1942	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	4	12	theme	N-linked	908:915	arg1	glycopeptides					924:936	N-linked intact glycopeptides	908:936	N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients	908:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	1	13	theme	hyperplasia	309:319	arg1	patients					327:334	benign prostatic hyperplasia (BPH) patients	292:334	benign prostatic hyperplasia (BPH) patients	292:334	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	9	14	theme	other	1983:1987	arg1	auROC					2001:2005	auROC = 0.853	2001:2013	auROC = 0.853	2001:2013	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	9	14	theme	other	1983:1987	arg1	indicators					1989:1998	other indicators	1983:1998	other indicators (auROC = 0.853)	1983:2014	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	3	15	theme	characterizing	607:620	arg1	glycopeptides					638:650	characterizing N-linked intact glycopeptides	607:650	characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information	607:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	7	16	dep	=	1623:1623	arg1	5.74					1625:1628	5.74	1625:1628	5.74	1625:1628	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	1	17	theme	biomarkers	216:225	arg1	necessary					339:347	necessary	339:347	necessary	339:347	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	1	17	theme	biomarkers	216:225	arg1	discovery					184:192	The discovery	180:192	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients	180:334	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	3	18	from	pathogenesis	786:797	arg1	discrimination					849:862	preoperative discrimination	836:862	preoperative discrimination of BPH from PCa	836:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	0	19	theme	prostatic	124:132	arg1	hyperplasia					134:144	benign prostatic hyperplasia	117:144	benign prostatic hyperplasia for diagnosis pre-stratification	117:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	3	20	with	structure	692:700	arg1	information					721:731	specific site information	707:731	specific site information	707:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	4	21	theme	BPH	983:985	arg1	patients					987:994	45 BPH patients	980:994	45 BPH patients	980:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	0	22	from	patients	80:87	arg1	signatures					49:58	N-linked intact glycopeptide signatures	20:58	N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	20:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	0	22	from	patients	80:87	arg1	IgGs					70:73	plasma IgGs	63:73	plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	63:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	9	23	theme	=	2007:2007	arg1	auROC					2001:2005	auROC = 0.853	2001:2013	auROC = 0.853	2001:2013	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	9	23	theme	=	2007:2007	arg1	indicators					1989:1998	other indicators	1983:1998	other indicators (auROC = 0.853)	1983:2014	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	3	24	theme	preoperative	836:847	arg1	discrimination					849:862	preoperative discrimination	836:862	preoperative discrimination of BPH from PCa	836:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	1	25	theme	prostate	254:261	arg1	PCa					274:276	PCa	274:276	PCa	274:276	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	1	25	theme	prostate	254:261	arg1	carcinoma					263:271	prostate carcinoma	254:271	prostate carcinoma (PCa) patients	254:286	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	9	26	theme	prediction	1828:1837	arg1	auROC					1851:1855	auROC = 0.702	1851:1863	auROC = 0.702	1851:1863	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	9	26	theme	prediction	1828:1837	arg1	capability					1839:1848	a more powerful prediction capability	1812:1848	a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681)	1812:1942	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	3	27	theme	signatures	822:831	arg1	discovery					803:811	discovery	803:811	discovery	803:811	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	3	27	theme	signatures	822:831	arg1	pathogenesis					786:797	molecular pathogenesis	776:797	molecular pathogenesis	776:797	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	1	28	theme	life	426:429	arg1	quality					415:421	quality	415:421	quality of life	415:429	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	3	29	theme	intact	631:636	arg1	glycopeptides					638:650	characterizing N-linked intact glycopeptides	607:650	characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information	607:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	4	30	theme	PCa	963:965	arg1	patients					967:974	51 PCa patients	960:974	51 PCa patients	960:974	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	6	31	theme	different	1427:1435	arg1	subclasses					1441:1450	different IgG subclasses	1427:1450	different IgG subclasses	1427:1450	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	7	32	theme	N-glycopeptide	1472:1485	arg1	EEQFNSTFR					1498:1506	EEQFNSTFR	1498:1506	EEQFNSTFR (H5N5S1)	1498:1515	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	7	32	theme	N-glycopeptide	1472:1485	arg1	IgG2-GP09					1487:1495	the N-glycopeptide IgG2-GP09	1468:1495	the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1))	1468:1516	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	10	33	theme	N-linked	2059:2066	arg1	glycopeptides					2075:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	0	34	theme	IgGs	70:73	arg1	signatures					49:58	N-linked intact glycopeptide signatures	20:58	N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	20:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	4	35	theme	hydrophilic	1043:1053	arg1	chromatography					1074:1087	hydrophilic interaction liquid chromatography	1043:1087	hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS	1043:1136	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	7	36	dep	patients	1599:1606	arg1	=					1633:1633	=	1633:1633	=	1633:1633	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	3	37	from	signatures	822:831	arg1	PCa					876:878	PCa	876:878	PCa	876:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	3	37	from	signatures	822:831	arg1	discrimination					849:862	preoperative discrimination	836:862	preoperative discrimination of BPH from PCa	836:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	10	38	theme	non-invasive	2108:2119	arg1	monitoring					2121:2130	non-invasive monitoring	2108:2130	non-invasive monitoring	2108:2130	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	4	39	from	glycopeptides	924:936	arg1	patients					987:994	45 BPH patients	980:994	45 BPH patients	980:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	4	39	from	glycopeptides	924:936	arg1	patients					967:974	51 PCa patients	960:974	51 PCa patients	960:974	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	9	40	theme	index	1916:1920	arg1	auROC					1929:1933	auROC = 0.681	1929:1941	auROC = 0.681	1929:1941	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	9	40	theme	index	1916:1920	arg1	t-PSA					1922:1926	the clinical index t-PSA	1903:1926	the clinical index t-PSA (auROC = 0.681)	1903:1942	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	2	41	theme	immunological	554:566	arg1	diseases					568:575	immunological diseases	554:575	immunological diseases	554:575	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	0	42	theme	prostate	94:101	arg1	carcinoma					103:111	prostate carcinoma	94:111	prostate carcinoma	94:111	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	1	43	theme	biopsies	373:380	arg1	burden					363:368	the burden	359:368	the burden of biopsies	359:380	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	4	44	theme	developed	1003:1011	arg1	method					1030:1035	our developed N-glycoproteomic method	999:1035	our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS	999:1136	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	8	45	from	changes	1744:1750	arg1	concentrations					1763:1776	the IgG concentrations	1755:1776	the IgG concentrations	1755:1776	Additionally, the variations in these N-linked intact glycopeptide abundances were not caused by the changes in the IgG concentrations.					
32568312	4	46	from	patients	987:994	arg1	glycopeptides					924:936	N-linked intact glycopeptides	908:936	N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients	908:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	4	46	from	patients	987:994	arg1	IgGs					950:953	purified IgGs	941:953	purified IgGs from 51 PCa patients and 45 BPH patients	941:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	2	47	theme	molecules	509:517	arg1	glycosylation					471:483	abnormal glycosylation	462:483	abnormal glycosylation of immunoglobulin gamma molecules (IgGs)	462:524	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	5	48	theme	plasma	1278:1283	arg1	IgG					1285:1287	plasma IgG subclass-specific	1278:1305	plasma IgG subclass-specific	1278:1305	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	4	49	theme	resolution	1118:1127	arg1	LC-MS/MS					1129:1136	high resolution LC-MS/MS	1113:1136	high resolution LC-MS/MS	1113:1136	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	0	50	theme	N-linked	20:27	arg1	signatures					49:58	N-linked intact glycopeptide signatures	20:58	N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	20:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	5	51	theme	subclass-specific	1289:1305	arg1	IgG					1285:1287	plasma IgG subclass-specific	1278:1305	plasma IgG subclass-specific	1278:1305	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	3	52	link	N-linked	622:629	arg1	glycopeptides					638:650	characterizing N-linked intact glycopeptides	607:650	characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information	607:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	0	53	theme	glycopeptide	36:47	arg1	signatures					49:58	N-linked intact glycopeptide signatures	20:58	N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	20:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	2	54	theme	gamma	503:507	arg1	IgGs					520:523	IgGs	520:523	IgGs	520:523	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	2	54	theme	gamma	503:507	arg1	molecules					509:517	immunoglobulin gamma molecules	488:517	immunoglobulin gamma molecules (IgGs)	488:524	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	2	55	theme	immunoglobulin	488:501	arg1	IgGs					520:523	IgGs	520:523	IgGs	520:523	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	2	55	theme	immunoglobulin	488:501	arg1	molecules					509:517	immunoglobulin gamma molecules	488:517	immunoglobulin gamma molecules (IgGs)	488:524	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	3	56	theme	N-glycan	683:690	arg1	structure					692:700	the N-glycan structure	679:700	the N-glycan structure with specific site information	679:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	7	57	theme	=	1623:1623	arg1	ratio					1617:1621	PCa/BPH ratio	1609:1621	PCa/BPH ratio = 5.74	1609:1628	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	9	58	theme	powerful	1819:1826	arg1	auROC					1851:1855	auROC = 0.702	1851:1863	auROC = 0.702	1851:1863	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	9	58	theme	powerful	1819:1826	arg1	capability					1839:1848	a more powerful prediction capability	1812:1848	a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681)	1812:1942	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	1	59	theme	carcinoma	263:271	arg1	patients					279:286	prostate carcinoma (PCa) patients	254:286	prostate carcinoma (PCa) patients	254:286	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	4	60	theme	liquid	1067:1072	arg1	chromatography					1074:1087	hydrophilic interaction liquid chromatography	1043:1087	hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS	1043:1136	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	7	61	theme	PCa/BPH	1609:1615	arg1	ratio					1617:1621	PCa/BPH ratio	1609:1621	PCa/BPH ratio = 5.74	1609:1628	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	2	62	gly	glycosylation	471:483	arg1	IgGs					520:523	IgGs	520:523	IgGs	520:523	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	2	62	gly	glycosylation	471:483	arg1	molecules					509:517	immunoglobulin gamma molecules	488:517	immunoglobulin gamma molecules (IgGs)	488:524	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	8	63	theme	N-linked	1681:1688	arg1	abundances					1710:1719	these N-linked intact glycopeptide abundances	1675:1719	these N-linked intact glycopeptide abundances	1675:1719	Additionally, the variations in these N-linked intact glycopeptide abundances were not caused by the changes in the IgG concentrations.					
32568312	3	64	theme	site	716:719	arg1	information					721:731	specific site information	707:731	specific site information	707:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	4	65	gly	glycopeptides	924:936	arg2	glycopeptides					924:936	N-linked intact glycopeptides	908:936	N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients	908:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	4	65	gly	glycopeptides	924:936	arg1	IgGs					950:953	purified IgGs	941:953	purified IgGs from 51 PCa patients and 45 BPH patients	941:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	7	66	dep	=	1633:1633	arg1	ratio					1617:1621	PCa/BPH ratio	1609:1621	PCa/BPH ratio = 5.74	1609:1628	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	10	67	theme	expressed	2049:2057	arg1	glycopeptides					2075:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	8	68	theme	glycopeptide	1697:1708	arg1	abundances					1710:1719	these N-linked intact glycopeptide abundances	1675:1719	these N-linked intact glycopeptide abundances	1675:1719	Additionally, the variations in these N-linked intact glycopeptide abundances were not caused by the changes in the IgG concentrations.					
32568312	9	69	theme	=	1857:1857	arg1	auROC					1851:1855	auROC = 0.702	1851:1863	auROC = 0.702	1851:1863	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	9	69	theme	=	1857:1857	arg1	capability					1839:1848	a more powerful prediction capability	1812:1848	a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681)	1812:1942	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	5	70	from	information	1263:1273	arg1	IgG					1285:1287	plasma IgG subclass-specific	1278:1305	plasma IgG subclass-specific	1278:1305	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	5	70	from	information	1263:1273	arg1	N-glycosylation					1325:1339	site-specific N-glycosylation	1311:1339	site-specific N-glycosylation	1311:1339	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	8	71	theme	IgG	1759:1761	arg1	concentrations					1763:1776	the IgG concentrations	1755:1776	the IgG concentrations	1755:1776	Additionally, the variations in these N-linked intact glycopeptide abundances were not caused by the changes in the IgG concentrations.					
32568312	5	72	theme	site-specific	1311:1323	arg1	N-glycosylation					1325:1339	site-specific N-glycosylation	1311:1339	site-specific N-glycosylation	1311:1339	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	4	73	theme	intact	917:922	arg1	glycopeptides					924:936	N-linked intact glycopeptides	908:936	N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients	908:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	10	74	theme	diseases	2167:2174	arg1	pre-stratification					2136:2153	pre-stratification	2136:2153	pre-stratification	2136:2153	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	10	74	theme	diseases	2167:2174	arg1	monitoring					2121:2130	non-invasive monitoring	2108:2130	non-invasive monitoring	2108:2130	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	6	75	theme	intact	1399:1404	arg1	result					1347:1352	a result	1345:1352	a result	1345:1352	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	6	75	theme	intact	1399:1404	arg1	glycopeptides					1406:1418	four aberrantly expressed N-linked intact glycopeptides	1364:1418	four aberrantly expressed N-linked intact glycopeptides	1364:1418	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	0	76	with	patients	80:87	arg1	carcinoma					103:111	prostate carcinoma	94:111	prostate carcinoma	94:111	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	1	77	theme	benign	292:297	arg1	BPH					322:324	BPH	322:324	BPH	322:324	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	1	77	theme	benign	292:297	arg1	hyperplasia					309:319	benign prostatic hyperplasia	292:319	benign prostatic hyperplasia (BPH) patients	292:334	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	3	78	theme	pathogenesis	786:797	arg1	understanding					755:767	a better understanding	746:767	a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa	746:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	6	79	theme	expressed	1380:1388	arg1	result					1347:1352	a result	1345:1352	a result	1345:1352	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	6	79	theme	expressed	1380:1388	arg1	glycopeptides					1406:1418	four aberrantly expressed N-linked intact glycopeptides	1364:1418	four aberrantly expressed N-linked intact glycopeptides	1364:1418	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	8	80	from	variations	1661:1670	arg1	abundances					1710:1719	these N-linked intact glycopeptide abundances	1675:1719	these N-linked intact glycopeptide abundances	1675:1719	Additionally, the variations in these N-linked intact glycopeptide abundances were not caused by the changes in the IgG concentrations.					
32568312	1	81	theme	non-invasive	203:214	arg1	biomarkers					216:225	novel non-invasive biomarkers	197:225	novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients	197:334	The discovery of novel non-invasive biomarkers for discriminating between prostate carcinoma (PCa) patients and benign prostatic hyperplasia (BPH) patients is necessary to reduce the burden of biopsies, avoid overdiagnosis and improve quality of life.					
32568312	5	82	theme	MaxQuant	1223:1230	arg1	software					1232:1239	MaxQuant software	1223:1239	MaxQuant software	1223:1239	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	5	83	gly	glycopeptides	1188:1200	arg2	glycopeptides					1188:1200	the N-linked intact glycopeptides	1168:1200	the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software	1168:1239	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	3	84	theme	novel	816:820	arg1	signatures					822:831	novel signatures	816:831	novel signatures in preoperative discrimination of BPH from PCa	816:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	7	85	gly	N-glycopeptide	1472:1485	arg2	N-glycopeptide					1472:1485	the N-glycopeptide IgG2-GP09	1468:1495	the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1))	1468:1516	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	2	86	theme	prostate	581:588	arg1	diseases					590:597	prostate diseases	581:597	prostate diseases	581:597	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	3	87	gly	glycopeptides	638:650	arg1	IgGs					655:658	IgGs	655:658	IgGs that correspond to the N-glycan structure with specific site information	655:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	3	87	gly	glycopeptides	638:650	arg2	glycopeptides					638:650	characterizing N-linked intact glycopeptides	607:650	characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information	607:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	4	88	link	N-linked	908:915	arg1	glycopeptides					924:936	N-linked intact glycopeptides	908:936	N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients	908:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	3	89	theme	discovery	803:811	arg1	understanding					755:767	a better understanding	746:767	a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa	746:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	0	90	theme	diagnosis	150:158	arg1	pre-stratification					160:177	diagnosis pre-stratification	150:177	diagnosis pre-stratification	150:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	10	91	link	N-linked	2059:2066	arg1	glycopeptides					2075:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	4	92	from	patients	967:974	arg1	glycopeptides					924:936	N-linked intact glycopeptides	908:936	N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients	908:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	4	92	from	patients	967:974	arg1	IgGs					950:953	purified IgGs	941:953	purified IgGs from 51 PCa patients and 45 BPH patients	941:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	3	93	theme	N-linked	622:629	arg1	glycopeptides					638:650	characterizing N-linked intact glycopeptides	607:650	characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information	607:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	4	94	theme	IgGs	950:953	arg1	glycopeptides					924:936	N-linked intact glycopeptides	908:936	N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients	908:994	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	8	95	link	N-linked	1681:1688	arg1	abundances					1710:1719	these N-linked intact glycopeptide abundances	1675:1719	these N-linked intact glycopeptide abundances	1675:1719	Additionally, the variations in these N-linked intact glycopeptide abundances were not caused by the changes in the IgG concentrations.					
32568312	6	96	theme	IgG	1437:1439	arg1	subclasses					1441:1450	different IgG subclasses	1427:1450	different IgG subclasses	1427:1450	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	0	97	with	hyperplasia	134:144	arg1	carcinoma					103:111	prostate carcinoma	94:111	prostate carcinoma	94:111	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	10	98	theme	intact	2068:2073	arg1	glycopeptides					2075:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	5	99	theme	quantitative	1143:1154	arg1	analysis					1156:1163	The quantitative analysis	1139:1163	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software	1139:1239	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	3	100	theme	IgGs	655:658	arg1	glycopeptides					638:650	characterizing N-linked intact glycopeptides	607:650	characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information	607:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	6	101	gly	glycopeptides	1406:1418	arg2	result					1347:1352	a result	1345:1352	a result	1345:1352	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	6	101	gly	glycopeptides	1406:1418	arg2	glycopeptides					1406:1418	four aberrantly expressed N-linked intact glycopeptides	1364:1418	four aberrantly expressed N-linked intact glycopeptides	1364:1418	As a result, we found four aberrantly expressed N-linked intact glycopeptides across different IgG subclasses.					
32568312	4	102	theme	N-glycoproteomic	1013:1028	arg1	method					1030:1035	our developed N-glycoproteomic method	999:1035	our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS	999:1136	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	3	103	theme	BPH	867:869	arg1	discrimination					849:862	preoperative discrimination	836:862	preoperative discrimination of BPH from PCa	836:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	5	104	link	N-linked	1172:1179	arg1	glycopeptides					1188:1200	the N-linked intact glycopeptides	1168:1200	the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software	1168:1239	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	5	105	theme	intact	1181:1186	arg1	glycopeptides					1188:1200	the N-linked intact glycopeptides	1168:1200	the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software	1168:1239	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	10	106	gly	glycopeptides	2075:2087	arg2	glycopeptides					2075:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	7	107	from	patients	1563:1570	arg1	plasma					1547:1552	plasma	1547:1552	plasma from PCa patients	1547:1570	In particular, the N-glycopeptide IgG2-GP09 (EEQFNSTFR (H5N5S1)) was dramatically elevated in plasma from PCa patients, compared with that in BPH patients (PCa/BPH ratio = 5.74, p = 0.001).					
32568312	3	108	from	discrimination	849:862	arg1	discovery					803:811	discovery	803:811	discovery	803:811	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	3	108	from	discrimination	849:862	arg1	pathogenesis					786:797	molecular pathogenesis	776:797	molecular pathogenesis	776:797	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	0	109	theme	benign	117:122	arg1	hyperplasia					134:144	benign prostatic hyperplasia	117:144	benign prostatic hyperplasia for diagnosis pre-stratification	117:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	5	110	theme	glycopeptides	1188:1200	arg1	analysis					1156:1163	The quantitative analysis	1139:1163	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software	1139:1239	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	0	111	from	signatures	49:58	arg1	patients					80:87	patients	80:87	patients with prostate carcinoma	80:111	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	0	111	from	signatures	49:58	arg1	hyperplasia					134:144	benign prostatic hyperplasia	117:144	benign prostatic hyperplasia for diagnosis pre-stratification	117:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	3	112	from	discovery	803:811	arg1	discrimination					849:862	preoperative discrimination	836:862	preoperative discrimination of BPH from PCa	836:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	10	113	contain	have	2089:2092	arg2	potential					2094:2102	potential	2094:2102	potential for non-invasive monitoring and pre-stratification of prostate diseases	2094:2174	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	10	113	contain	have	2089:2092	arg1	glycopeptides					2075:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	these abnormally expressed N-linked intact glycopeptides	2032:2087	In conclusion, these abnormally expressed N-linked intact glycopeptides have potential for non-invasive monitoring and pre-stratification of prostate diseases.					
32568312	2	114	theme	abnormal	462:469	arg1	glycosylation					471:483	abnormal glycosylation	462:483	abnormal glycosylation of immunoglobulin gamma molecules (IgGs)	462:524	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	9	115	with	combination	1966:1976	arg1	auROC					2001:2005	auROC = 0.853	2001:2013	auROC = 0.853	2001:2013	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	9	115	with	combination	1966:1976	arg1	indicators					1989:1998	other indicators	1983:1998	other indicators (auROC = 0.853)	1983:2014	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	0	116	theme	intact	29:34	arg1	signatures					49:58	N-linked intact glycopeptide signatures	20:58	N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	20:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	4	117	theme	high	1113:1116	arg1	LC-MS/MS					1129:1136	high resolution LC-MS/MS	1113:1136	high resolution LC-MS/MS	1113:1136	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	2	118	theme	Previous	432:439	arg1	studies					441:447	Previous studies	432:447	Previous studies	432:447	Previous studies suggest that abnormal glycosylation of immunoglobulin gamma molecules (IgGs) is strongly associated with immunological diseases and prostate diseases.					
32568312	0	119	theme	signatures	49:58	arg1	Characterization					0:15	Characterization	0:15	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.	0:178	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	4	120	theme	interaction	1055:1065	arg1	chromatography					1074:1087	hydrophilic interaction liquid chromatography	1043:1087	hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS	1043:1136	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	0	121	theme	plasma	63:68	arg1	IgGs					70:73	plasma IgGs	63:73	plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	63:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	5	122	theme	quantitative	1250:1261	arg1	information					1263:1273	quantitative information	1250:1273	quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation	1250:1339	The quantitative analysis of the N-linked intact glycopeptides using pGlyco 2.0 and MaxQuant software provided quantitative information on plasma IgG subclass-specific and site-specific N-glycosylation.					
32568312	8	123	gly	glycopeptide	1697:1708	arg2	glycopeptide					1697:1708	these N-linked intact glycopeptide abundances	1675:1719	these N-linked intact glycopeptide abundances	1675:1719	Additionally, the variations in these N-linked intact glycopeptide abundances were not caused by the changes in the IgG concentrations.					
32568312	4	124	theme	chromatography	1074:1087	arg1	enrichment					1089:1098	hydrophilic interaction liquid chromatography enrichment	1043:1098	hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS	1043:1136	In this study, we profiled N-linked intact glycopeptides of purified IgGs from 51 PCa patients and 45 BPH patients by our developed N-glycoproteomic method using hydrophilic interaction liquid chromatography enrichment coupled with high resolution LC-MS/MS.					
32568312	3	125	dep	pathogenesis	786:797	arg1	the					772:774	the	772:774	the	772:774	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	0	126	gly	glycopeptide	36:47	arg2	glycopeptide					36:47	N-linked intact glycopeptide signatures	20:58	N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	20:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	9	127	theme	=	1935:1935	arg1	auROC					1929:1933	auROC = 0.681	1929:1941	auROC = 0.681	1929:1941	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	9	127	theme	=	1935:1935	arg1	t-PSA					1922:1926	the clinical index t-PSA	1903:1926	the clinical index t-PSA (auROC = 0.681)	1903:1942	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	8	128	theme	intact	1690:1695	arg1	abundances					1710:1719	these N-linked intact glycopeptide abundances	1675:1719	these N-linked intact glycopeptide abundances	1675:1719	Additionally, the variations in these N-linked intact glycopeptide abundances were not caused by the changes in the IgG concentrations.					
32568312	3	129	from	PCa	876:878	arg1	signatures					822:831	novel signatures	816:831	novel signatures in preoperative discrimination of BPH from PCa	816:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	3	129	from	PCa	876:878	arg1	discrimination					849:862	preoperative discrimination	836:862	preoperative discrimination of BPH from PCa	836:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	3	130	theme	specific	707:714	arg1	information					721:731	specific site information	707:731	specific site information	707:731	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
32568312	9	131	theme	clinical	1907:1914	arg1	auROC					1929:1933	auROC = 0.681	1929:1941	auROC = 0.681	1929:1941	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	9	131	theme	clinical	1907:1914	arg1	t-PSA					1922:1926	the clinical index t-PSA	1903:1926	the clinical index t-PSA (auROC = 0.681)	1903:1942	Furthermore, IgG2-GP09 displayed a more powerful prediction capability (auROC = 0.702) for distinguishing PCa from BPH than the clinical index t-PSA (auROC = 0.681) when used alone or in combination with other indicators (auROC = 0.853).					
32568312	0	132	from	hyperplasia	134:144	arg1	signatures					49:58	N-linked intact glycopeptide signatures	20:58	N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	20:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	0	132	from	hyperplasia	134:144	arg1	IgGs					70:73	plasma IgGs	63:73	plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification	63:177	Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification.					
32568312	3	133	theme	better	748:753	arg1	understanding					755:767	a better understanding	746:767	a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa	746:878	Hence, characterizing N-linked intact glycopeptides of IgGs that correspond to the N-glycan structure with specific site information might enable a better understanding of the molecular pathogenesis and discovery of novel signatures in preoperative discrimination of BPH from PCa.					
31676481	2	0	theme	acid	344:347	arg1	profiles					349:356	characteristic short-chain fatty acid profiles	311:356	characteristic short-chain fatty acid profiles	311:356	The microbiota degrades and ferments substrates, producing characteristic short-chain fatty acid profiles.					
31676481	10	1	theme	gut	2022:2024	arg1	microbiota					2026:2035	the human gut microbiota	2012:2035	the human gut microbiota	2012:2035	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	12	2	theme	certain	2330:2336	arg1	polysaccharides					2338:2352	certain polysaccharides	2330:2352	certain polysaccharides	2330:2352	The bacteria showed preferential use of certain polysaccharides over others for growth, and this influenced their fermentation output qualitatively.					
31676481	13	3	theme	microbial	2514:2522	arg1	community					2524:2532	the gut microbial community	2506:2532	the gut microbial community	2506:2532	These kinds of studies are essential in developing concepts of how the gut microbial community shares habitat resources, directly and indirectly, when presented with mixtures of polysaccharides that are found in human diets.					
31676481	1	4	theme	humans	244:249	arg1	microbiota					230:239	the colonic microbiota	218:239	the colonic microbiota of humans	218:249	Dietary fiber provides growth substrates for bacterial species that belong to the colonic microbiota of humans.					
31676481	9	5	theme	polysaccharide	1435:1448	arg1	mixtures					1450:1457	polysaccharide mixtures	1435:1457	polysaccharide mixtures	1435:1457	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	9	6	theme	ecological	1622:1631	arg1	community					1633:1641	a synthetic ecological community	1610:1641	a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose	1610:1740	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	11	7	theme	detectable	2234:2243	arg1	products					2225:2232	acidic fermentation products	2205:2232	acidic fermentation products detectable in human feces	2205:2258	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces were used in the experiments.					
31676481	3	8	from	composition	485:495	arg1	diverse					474:480	diverse	474:480	diverse	474:480	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	5	9	dep	extent	677:682	arg1	the					673:675	the	673:675	the	673:675	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	11	10	theme	fermentation	2212:2223	arg1	products					2225:2232	acidic fermentation products	2205:2232	acidic fermentation products detectable in human feces	2205:2258	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces were used in the experiments.					
31676481	10	11	theme	human	1903:1907	arg1	physiology.IMPORTANCE					1909:1929	human physiology.IMPORTANCE	1903:1929	human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber	1903:2126	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	3	12	from	structure	501:509	arg1	diverse					474:480	diverse	474:480	diverse	474:480	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	9	13	theme	SCFAs	1409:1413	arg1	proportion					1389:1398	a proportion	1387:1398	a proportion of total SCFAs	1387:1413	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	6	14	theme	short-chain	1144:1154	arg1	SCFAs					1169:1173	SCFAs	1169:1173	SCFAs	1169:1173	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	6	14	theme	short-chain	1144:1154	arg1	acids					1162:1166	short-chain fatty acids	1144:1166	short-chain fatty acids (SCFAs)	1144:1174	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	13	15	theme	studies	2454:2460	arg1	kinds					2445:2449	These kinds	2439:2449	These kinds of studies	2439:2460	These kinds of studies are essential in developing concepts of how the gut microbial community shares habitat resources, directly and indirectly, when presented with mixtures of polysaccharides that are found in human diets.					
31676481	14	16	theme	human	2780:2784	arg1	microbiota					2786:2795	the human microbiota	2776:2795	the human microbiota so as to support measures to reduce metabolic conditions such as obesity	2776:2868	The concepts are required in planning dietary interventions that might correct imbalances in the functioning of the human microbiota so as to support measures to reduce metabolic conditions such as obesity.					
31676481	10	17	theme	polysaccharides	2077:2091	arg1	mixtures					2059:2066	mixtures	2059:2066	mixtures of plant polysaccharides such as are found in dietary fiber	2059:2126	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	5	18	theme	plant	728:732	arg1	arabinoxylan					751:762	arabinoxylan	751:762	arabinoxylan	751:762	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	18	theme	plant	728:732	arg1	polysaccharides					734:748	plant polysaccharides	728:748	plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin)	728:797	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	18	theme	plant	728:732	arg1	β-glucan					777:784	β-glucan	777:784	β-glucan	777:784	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	18	theme	plant	728:732	arg1	pectin					791:796	pectin	791:796	pectin	791:796	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	18	theme	plant	728:732	arg1	xyloglucan					765:774	xyloglucan	765:774	xyloglucan	765:774	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	19	theme	species	846:852	arg1	coculture					818:826	a coculture	816:826	a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula)	816:983	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	7	20	from	glycans	1196:1202	arg1	xyloglucan					1235:1244	xyloglucan	1235:1244	xyloglucan	1235:1244	B. ovatus utilized glycans in the order β-glucan, pectin, xyloglucan, and arabinoxylan, whereas B. longum subsp.					
31676481	7	20	from	glycans	1196:1202	arg1	arabinoxylan					1251:1262	arabinoxylan	1251:1262	arabinoxylan	1251:1262	B. ovatus utilized glycans in the order β-glucan, pectin, xyloglucan, and arabinoxylan, whereas B. longum subsp.					
31676481	7	20	from	glycans	1196:1202	arg1	pectin					1227:1232	pectin	1227:1232	pectin	1227:1232	B. ovatus utilized glycans in the order β-glucan, pectin, xyloglucan, and arabinoxylan, whereas B. longum subsp.					
31676481	7	20	from	glycans	1196:1202	arg1	β-glucan					1217:1224	the order β-glucan	1207:1224	the order β-glucan	1207:1224	B. ovatus utilized glycans in the order β-glucan, pectin, xyloglucan, and arabinoxylan, whereas B. longum subsp.					
31676481	10	21	theme	dietary	2114:2120	arg1	fiber					2122:2126	dietary fiber	2114:2126	dietary fiber	2114:2126	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	5	22	theme	preferential	688:699	arg1	order					701:705	preferential order	688:705	preferential order	688:705	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	23	dep	polysaccharides	734:748	arg1	arabinoxylan					751:762	arabinoxylan	751:762	arabinoxylan	751:762	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	23	dep	polysaccharides	734:748	arg1	polysaccharides					734:748	plant polysaccharides	728:748	plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin)	728:797	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	23	dep	polysaccharides	734:748	arg1	β-glucan					777:784	β-glucan	777:784	β-glucan	777:784	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	23	dep	polysaccharides	734:748	arg1	pectin					791:796	pectin	791:796	pectin	791:796	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	23	dep	polysaccharides	734:748	arg1	xyloglucan					765:774	xyloglucan	765:774	xyloglucan	765:774	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	0	24	theme	Pectin	132:137	arg1	Mixtures					80:87	Fed Mixtures	76:87	Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin	76:137	Substrate Use Prioritization by a Coculture of Five Species of Gut Bacteria Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin.					
31676481	6	25	theme	human	1019:1023	arg1	microbiota					1029:1038	the human gut microbiota	1015:1038	the human gut microbiota	1015:1038	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	8	26	theme	longum	1290:1295	arg1	utilization					1297:1307	longum utilization	1290:1307	longum utilization	1290:1307	longum utilization was in the order arabinoxylan, arabinan, pectin, and β-glucan.					
31676481	0	27	theme	Species	52:58	arg1	Prioritization					14:27	Substrate Use Prioritization	0:27	Substrate Use Prioritization by a Coculture of Five Species of Gut	0:65	Substrate Use Prioritization by a Coculture of Five Species of Gut Bacteria Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin.					
31676481	4	28	with	microbiota	579:588	arg1	mixtures					595:602	mixtures	595:602	mixtures of plant polysaccharides of various types and complexity	595:659	Thus, depending on plant sources, dietary fiber daily presents the microbiota with mixtures of plant polysaccharides of various types and complexity.					
31676481	10	29	dep	physiology.IMPORTANCE	1909:1929	arg1	addresses					1942:1950	addresses	1942:1950	addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber	1942:2126	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	14	30	theme	metabolic	2833:2841	arg1	conditions					2843:2852	metabolic conditions	2833:2852	metabolic conditions such as obesity	2833:2868	The concepts are required in planning dietary interventions that might correct imbalances in the functioning of the human microbiota so as to support measures to reduce metabolic conditions such as obesity.					
31676481	14	30	theme	metabolic	2833:2841	arg1	obesity					2862:2868	obesity	2862:2868	obesity	2862:2868	The concepts are required in planning dietary interventions that might correct imbalances in the functioning of the human microbiota so as to support measures to reduce metabolic conditions such as obesity.					
31676481	9	31	dep	ovatus	1527:1532	arg1	derived					1602:1608	derived	1602:1608	derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose	1602:1740	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	0	32	theme	Gut	63:65	arg1	Species					52:58	Five Species	47:58	Five Species of Gut	47:65	Substrate Use Prioritization by a Coculture of Five Species of Gut Bacteria Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin.					
31676481	13	33	theme	human	2651:2655	arg1	diets					2657:2661	human diets	2651:2661	human diets	2651:2661	These kinds of studies are essential in developing concepts of how the gut microbial community shares habitat resources, directly and indirectly, when presented with mixtures of polysaccharides that are found in human diets.					
31676481	9	34	theme	greater	1492:1498	arg1	production					1510:1519	greater succinate production	1492:1519	greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose	1492:1740	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	10	35	theme	bacterial	1975:1983	arg1	species					1985:1991	bacterial species	1975:1991	bacterial species	1975:1991	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	0	36	theme	Fed	76:78	arg1	Mixtures					80:87	Fed Mixtures	76:87	Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin	76:137	Substrate Use Prioritization by a Coculture of Five Species of Gut Bacteria Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin.					
31676481	13	37	located	found	2642:2646	arg2	polysaccharides					2617:2631	polysaccharides	2617:2631	polysaccharides that are found in human diets	2617:2661	These kinds of studies are essential in developing concepts of how the gut microbial community shares habitat resources, directly and indirectly, when presented with mixtures of polysaccharides that are found in human diets.					
31676481	13	37	located	found	2642:2646	arg1	diets					2657:2661	human diets	2651:2661	human diets	2651:2661	These kinds of studies are essential in developing concepts of how the gut microbial community shares habitat resources, directly and indirectly, when presented with mixtures of polysaccharides that are found in human diets.					
31676481	3	38	theme	plant	382:386	arg1	polysaccharides					409:423	plant cell wall-associated polysaccharides	382:423	plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure	382:509	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	0	39	theme	Substrate	0:8	arg1	Prioritization					14:27	Substrate Use Prioritization	0:27	Substrate Use Prioritization by a Coculture of Five Species of Gut	0:65	Substrate Use Prioritization by a Coculture of Five Species of Gut Bacteria Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin.					
31676481	7	40	used	utilized	1187:1194	arg2	ovatus					1180:1185	B. ovatus	1177:1185	B. ovatus	1177:1185	B. ovatus utilized glycans in the order β-glucan, pectin, xyloglucan, and arabinoxylan, whereas B. longum subsp.					
31676481	6	41	theme	biochemical	1053:1063	arg1	capacity					1065:1072	the biochemical capacity	1049:1072	the biochemical capacity	1049:1072	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	9	42	contain	carries	1648:1654	arg1	community					1633:1641	a synthetic ecological community	1610:1641	a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose	1610:1740	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	9	42	contain	carries	1648:1654	arg2	production					1665:1674	SCFA production	1660:1674	SCFA production by the common pathways used by bacterial species for this purpose	1660:1740	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	11	43	theme	bacterial	2134:2142	arg1	species					2144:2150	Five bacterial species	2129:2150	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces	2129:2258	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces were used in the experiments.					
31676481	12	44	theme	fermentation	2404:2415	arg1	output					2417:2422	their fermentation output	2398:2422	their fermentation output	2398:2422	The bacteria showed preferential use of certain polysaccharides over others for growth, and this influenced their fermentation output qualitatively.					
31676481	10	45	theme	emergent	1801:1808	arg1	properties					1810:1819	the emergent properties	1797:1819	the emergent properties of the gut ecosystem	1797:1840	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	7	46	theme	B.	1273:1274	arg1	subsp					1283:1287	B. longum subsp	1273:1287	B. longum subsp	1273:1287	B. ovatus utilized glycans in the order β-glucan, pectin, xyloglucan, and arabinoxylan, whereas B. longum subsp.					
31676481	3	47	theme	wall-associated	393:407	arg1	polysaccharides					409:423	plant cell wall-associated polysaccharides	382:423	plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure	382:509	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	4	48	theme	plant	531:535	arg1	sources					537:543	plant sources	531:543	plant sources	531:543	Thus, depending on plant sources, dietary fiber daily presents the microbiota with mixtures of plant polysaccharides of various types and complexity.					
31676481	2	49	theme	fatty	338:342	arg1	profiles					349:356	characteristic short-chain fatty acid profiles	311:356	characteristic short-chain fatty acid profiles	311:356	The microbiota degrades and ferments substrates, producing characteristic short-chain fatty acid profiles.					
31676481	10	50	theme	human	2016:2020	arg1	microbiota					2026:2035	the human gut microbiota	2012:2035	the human gut microbiota	2012:2035	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	11	51	from	detectable	2234:2243	arg1	feces					2254:2258	human feces	2248:2258	human feces	2248:2258	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces were used in the experiments.					
31676481	10	52	theme	gut	1828:1830	arg1	ecosystem					1832:1840	the gut ecosystem	1824:1840	the gut ecosystem	1824:1840	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	2	53	theme	characteristic	311:324	arg1	profiles					349:356	characteristic short-chain fatty acid profiles	311:356	characteristic short-chain fatty acid profiles	311:356	The microbiota degrades and ferments substrates, producing characteristic short-chain fatty acid profiles.					
31676481	5	54	used	utilized	804:811	arg2	mixtures					716:723	mixtures	716:723	mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin)	716:797	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	7	55	dep	B.	1273:1274	arg1	longum					1276:1281	longum	1276:1281	longum	1276:1281	B. ovatus utilized glycans in the order β-glucan, pectin, xyloglucan, and arabinoxylan, whereas B. longum subsp.					
31676481	10	56	dep	mixtures	2059:2066	arg1	found					2105:2109	found	2105:2109	are found in dietary fiber	2101:2126	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	4	57	theme	plant	607:611	arg1	polysaccharides					613:627	plant polysaccharides	607:627	plant polysaccharides of various types and complexity	607:659	Thus, depending on plant sources, dietary fiber daily presents the microbiota with mixtures of plant polysaccharides of various types and complexity.					
31676481	6	58	theme	microbiota	1029:1038	arg1	members					1004:1010	members	1004:1010	members of the human gut microbiota	1004:1038	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	6	58	theme	microbiota	1029:1038	arg1	species					992:998	These species	986:998	These species	986:998	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	1	59	theme	colonic	222:228	arg1	microbiota					230:239	the colonic microbiota	218:239	the colonic microbiota of humans	218:249	Dietary fiber provides growth substrates for bacterial species that belong to the colonic microbiota of humans.					
31676481	10	60	theme	microbiota	2026:2035	arg1	characteristic					1994:2007	characteristic	1994:2007	characteristic	1994:2007	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	12	61	theme	preferential	2310:2321	arg1	use					2323:2325	preferential use	2310:2325	preferential use of certain polysaccharides over others for growth	2310:2375	The bacteria showed preferential use of certain polysaccharides over others for growth, and this influenced their fermentation output qualitatively.					
31676481	13	62	theme	gut	2510:2512	arg1	community					2524:2532	the gut microbial community	2506:2532	the gut microbial community	2506:2532	These kinds of studies are essential in developing concepts of how the gut microbial community shares habitat resources, directly and indirectly, when presented with mixtures of polysaccharides that are found in human diets.					
31676481	9	63	theme	synthetic	1612:1620	arg1	community					1633:1641	a synthetic ecological community	1610:1641	a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose	1610:1740	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	10	64	theme	plant	2071:2075	arg1	polysaccharides					2077:2091	plant polysaccharides	2071:2091	plant polysaccharides	2071:2091	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	14	65	theme	dietary	2702:2708	arg1	interventions					2710:2722	planning dietary interventions	2693:2722	planning dietary interventions that might correct imbalances in the functioning of the human microbiota so as to support measures to reduce metabolic conditions such as obesity	2693:2868	The concepts are required in planning dietary interventions that might correct imbalances in the functioning of the human microbiota so as to support measures to reduce metabolic conditions such as obesity.					
31676481	1	66	theme	Dietary	140:146	arg1	fiber					148:152	Dietary fiber	140:152	Dietary fiber	140:152	Dietary fiber provides growth substrates for bacterial species that belong to the colonic microbiota of humans.					
31676481	3	67	contain	contains	373:380	arg1	fiber					367:371	Dietary fiber	359:371	Dietary fiber	359:371	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	3	67	contain	contains	373:380	arg2	polysaccharides					409:423	plant cell wall-associated polysaccharides	382:423	plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure	382:509	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	11	68	with	species	2144:2150	arg1	capacity					2161:2168	the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces	2157:2258	the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces	2157:2258	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces were used in the experiments.					
31676481	11	69	theme	acidic	2205:2210	arg1	products					2225:2232	acidic fermentation products	2205:2232	acidic fermentation products detectable in human feces	2205:2258	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces were used in the experiments.					
31676481	9	70	theme	total	1403:1407	arg1	SCFAs					1409:1413	total SCFAs	1403:1413	total SCFAs	1403:1413	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	6	71	theme	fatty	1156:1160	arg1	SCFAs					1169:1173	SCFAs	1169:1173	SCFAs	1169:1173	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	6	71	theme	fatty	1156:1160	arg1	acids					1162:1166	short-chain fatty acids	1144:1166	short-chain fatty acids (SCFAs)	1144:1174	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	0	72	theme	Xyloglucan	106:115	arg1	Mixtures					80:87	Fed Mixtures	76:87	Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin	76:137	Substrate Use Prioritization by a Coculture of Five Species of Gut Bacteria Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin.					
31676481	9	73	theme	SCFA	1660:1663	arg1	production					1665:1674	SCFA production	1660:1674	SCFA production by the common pathways used by bacterial species for this purpose	1660:1740	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	4	74	theme	types	640:644	arg1	polysaccharides					613:627	plant polysaccharides	607:627	plant polysaccharides of various types and complexity	607:659	Thus, depending on plant sources, dietary fiber daily presents the microbiota with mixtures of plant polysaccharides of various types and complexity.					
31676481	0	75	theme	β-Glucan	118:125	arg1	Mixtures					80:87	Fed Mixtures	76:87	Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin	76:137	Substrate Use Prioritization by a Coculture of Five Species of Gut Bacteria Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin.					
31676481	1	76	theme	growth	163:168	arg1	substrates					170:179	growth substrates	163:179	growth substrates for bacterial species that belong to the colonic microbiota of humans	163:249	Dietary fiber provides growth substrates for bacterial species that belong to the colonic microbiota of humans.					
31676481	5	77	theme	bacterial	836:844	arg1	species					846:852	five bacterial species	831:852	five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula)	831:983	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	4	78	theme	complexity	650:659	arg1	polysaccharides					613:627	plant polysaccharides	607:627	plant polysaccharides of various types and complexity	607:659	Thus, depending on plant sources, dietary fiber daily presents the microbiota with mixtures of plant polysaccharides of various types and complexity.					
31676481	11	79	from	feces	2254:2258	arg1	detectable					2234:2243	detectable	2234:2243	detectable	2234:2243	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces were used in the experiments.					
31676481	10	80	used	used	1770:1773	arg2	Systems					1743:1749	Systems	1743:1749	Systems like this	1743:1759	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	9	81	theme	common	1683:1688	arg1	pathways					1690:1697	the common pathways	1679:1697	the common pathways used by bacterial species for this purpose	1679:1740	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	6	82	theme	gut	1025:1027	arg1	microbiota					1029:1038	the human gut microbiota	1015:1038	the human gut microbiota	1015:1038	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	7	83	theme	order	1211:1215	arg1	β-glucan					1217:1224	the order β-glucan	1207:1224	the order β-glucan	1207:1224	B. ovatus utilized glycans in the order β-glucan, pectin, xyloglucan, and arabinoxylan, whereas B. longum subsp.					
31676481	9	84	theme	bacterial	1707:1715	arg1	species					1717:1723	bacterial species	1707:1723	bacterial species	1707:1723	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	13	85	theme	polysaccharides	2617:2631	arg1	mixtures					2605:2612	mixtures	2605:2612	mixtures of polysaccharides that are found in human diets	2605:2661	These kinds of studies are essential in developing concepts of how the gut microbial community shares habitat resources, directly and indirectly, when presented with mixtures of polysaccharides that are found in human diets.					
31676481	5	86	theme	polysaccharides	734:748	arg1	mixtures					716:723	mixtures	716:723	mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin)	716:797	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	9	87	theme	succinate	1500:1508	arg1	production					1510:1519	greater succinate production	1492:1519	greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose	1492:1740	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	3	88	theme	Dietary	359:365	arg1	fiber					367:371	Dietary fiber	359:371	Dietary fiber	359:371	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	11	89	used	used	2265:2268	arg2	species					2144:2150	Five bacterial species	2129:2150	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces	2129:2258	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces were used in the experiments.					
31676481	0	90	theme	Arabinoxylan	92:103	arg1	Mixtures					80:87	Fed Mixtures	76:87	Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin	76:137	Substrate Use Prioritization by a Coculture of Five Species of Gut Bacteria Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin.					
31676481	6	91	contain	have	1044:1047	arg2	capacity					1065:1072	the biochemical capacity	1049:1072	the biochemical capacity	1049:1072	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	6	91	contain	have	1044:1047	arg1	members					1004:1010	members	1004:1010	members of the human gut microbiota	1004:1038	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	6	91	contain	have	1044:1047	arg1	species					992:998	These species	986:998	These species	986:998	These species are members of the human gut microbiota and have the biochemical capacity, collectively, to degrade and ferment the polysaccharides and produce short-chain fatty acids (SCFAs).					
31676481	3	92	dep	polysaccharides	409:423	arg1	pectins					445:451	pectins	445:451	pectins	445:451	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	3	92	dep	polysaccharides	409:423	arg1	hemicelluloses					426:439	hemicelluloses	426:439	hemicelluloses	426:439	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	8	93	theme	order	1320:1324	arg1	arabinoxylan					1326:1337	the order arabinoxylan	1316:1337	the order arabinoxylan	1316:1337	longum utilization was in the order arabinoxylan, arabinan, pectin, and β-glucan.					
31676481	0	94	theme	Use	10:12	arg1	Prioritization					14:27	Substrate Use Prioritization	0:27	Substrate Use Prioritization by a Coculture of Five Species of Gut	0:65	Substrate Use Prioritization by a Coculture of Five Species of Gut Bacteria Fed Mixtures of Arabinoxylan, Xyloglucan, β-Glucan, and Pectin.					
31676481	3	95	theme	cell	388:391	arg1	polysaccharides					409:423	plant cell wall-associated polysaccharides	382:423	plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure	382:509	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	12	96	theme	polysaccharides	2338:2352	arg1	use					2323:2325	preferential use	2310:2325	preferential use of certain polysaccharides over others for growth	2310:2375	The bacteria showed preferential use of certain polysaccharides over others for growth, and this influenced their fermentation output qualitatively.					
31676481	10	97	dep	found	2105:2109	arg1	such					2093:2096	such	2093:2096	such	2093:2096	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	14	98	theme	planning	2693:2700	arg1	interventions					2710:2722	planning dietary interventions	2693:2722	planning dietary interventions that might correct imbalances in the functioning of the human microbiota so as to support measures to reduce metabolic conditions such as obesity	2693:2868	The concepts are required in planning dietary interventions that might correct imbalances in the functioning of the human microbiota so as to support measures to reduce metabolic conditions such as obesity.					
31676481	9	99	contain	contained	1459:1467	arg2	galactan					1469:1476	galactan	1469:1476	galactan	1469:1476	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	9	99	contain	contained	1459:1467	arg1	mixtures					1450:1457	polysaccharide mixtures	1435:1457	polysaccharide mixtures	1435:1457	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	11	100	theme	human	2248:2252	arg1	feces					2254:2258	human feces	2248:2258	human feces	2248:2258	Five bacterial species with the capacity to degrade polymers and/or produce acidic fermentation products detectable in human feces were used in the experiments.					
31676481	1	101	theme	bacterial	185:193	arg1	species					195:201	bacterial species	185:201	bacterial species that belong to the colonic microbiota of humans	185:249	Dietary fiber provides growth substrates for bacterial species that belong to the colonic microbiota of humans.					
31676481	9	102	theme	succinate	1552:1560	arg1	conversion					1538:1547	conversion	1538:1547	conversion of succinate to propionate by V. parvula	1538:1588	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	13	103	theme	habitat	2541:2547	arg1	resources					2549:2557	habitat resources	2541:2557	habitat resources	2541:2557	These kinds of studies are essential in developing concepts of how the gut microbial community shares habitat resources, directly and indirectly, when presented with mixtures of polysaccharides that are found in human diets.					
31676481	4	104	theme	dietary	546:552	arg1	fiber					554:558	dietary fiber	546:558	dietary fiber	546:558	Thus, depending on plant sources, dietary fiber daily presents the microbiota with mixtures of plant polysaccharides of various types and complexity.					
31676481	10	105	theme	ecosystem	1832:1840	arg1	properties					1810:1819	the emergent properties	1797:1819	the emergent properties of the gut ecosystem	1797:1840	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	3	106	from	diverse	474:480	arg1	structure					501:509	structure	501:509	structure	501:509	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	3	106	from	diverse	474:480	arg1	composition					485:495	composition	485:495	composition	485:495	Dietary fiber contains plant cell wall-associated polysaccharides (hemicelluloses and pectins) that are chemically diverse in composition and structure.					
31676481	2	107	theme	short-chain	326:336	arg1	profiles					349:356	characteristic short-chain fatty acid profiles	311:356	characteristic short-chain fatty acid profiles	311:356	The microbiota degrades and ferments substrates, producing characteristic short-chain fatty acid profiles.					
31676481	9	108	theme	V.	1579:1580	arg1	parvula					1582:1588	V. parvula	1579:1588	V. parvula	1579:1588	Propionate, as a proportion of total SCFAs, was augmented when polysaccharide mixtures contained galactan, resulting in greater succinate production by B. ovatus and conversion of succinate to propionate by V. parvula Overall, we derived a synthetic ecological community that carries out SCFA production by the common pathways used by bacterial species for this purpose.					
31676481	4	109	theme	polysaccharides	613:627	arg1	mixtures					595:602	mixtures	595:602	mixtures of plant polysaccharides of various types and complexity	595:659	Thus, depending on plant sources, dietary fiber daily presents the microbiota with mixtures of plant polysaccharides of various types and complexity.					
31676481	5	110	dep	species	846:852	arg1	elsdenii					929:936	Megasphaera elsdenii	917:936	Megasphaera elsdenii	917:936	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	110	dep	species	846:852	arg1	parvula					976:982	parvula	976:982	parvula	976:982	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	110	dep	species	846:852	arg1	longum					909:914	Bifidobacterium longum subspecies longum	875:914	Bifidobacterium longum subspecies longum	875:914	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	110	dep	species	846:852	arg1	ovatus					867:872	Bacteroides ovatus	855:872	Bacteroides ovatus	855:872	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	5	110	dep	species	846:852	arg1	gnavus					952:957	Ruminococcus gnavus	939:957	Ruminococcus gnavus	939:957	We studied the extent and preferential order in which mixtures of plant polysaccharides (arabinoxylan, xyloglucan, β-glucan, and pectin) were utilized by a coculture of five bacterial species (Bacteroides ovatus, Bifidobacterium longum subspecies longum, Megasphaera elsdenii, Ruminococcus gnavus, and Veillonella parvula).					
31676481	10	111	theme	characteristic	1994:2007	arg1	species					1985:1991	bacterial species	1975:1991	bacterial species	1975:1991	Systems like this might be used to predict changes to the emergent properties of the gut ecosystem when diet is altered, with the aim of beneficially affecting human physiology.IMPORTANCE This study addresses the question as to how bacterial species, characteristic of the human gut microbiota, collectively utilize mixtures of plant polysaccharides such as are found in dietary fiber.					
31676481	4	112	theme	various	632:638	arg1	types					640:644	various types	632:644	various types	632:644	Thus, depending on plant sources, dietary fiber daily presents the microbiota with mixtures of plant polysaccharides of various types and complexity.					
34420737	0	0	theme	aldononitrile	80:92	arg1	acetate					94:100	aldononitrile acetate	80:100	aldononitrile acetate	80:100	A novel LC-MS/MS method for complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring.					
34420737	9	1	theme	complex	1590:1596	arg1	pectins					1598:1604	complex pectins	1590:1604	complex pectins	1590:1604	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	4	2	from	composition	679:689	arg1	polysaccharides					775:789	plant-derived polysaccharides	761:789	plant-derived polysaccharides	761:789	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	3	theme	liquid	548:553	arg1	LC-MS/MS					596:603	LC-MS/MS	596:603	LC-MS/MS	596:603	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	3	theme	liquid	548:553	arg1	spectrometry					582:593	liquid chromatography-tandem mass spectrometry	548:593	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	548:604	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	5	4	theme	monitoring	885:894	arg1	parameters					902:911	the multiple reaction monitoring (MRM) parameters	863:911	the multiple reaction monitoring (MRM) parameters	863:911	In addition to discussing the possible byproducts, the study optimizes the multiple reaction monitoring (MRM) parameters and LC conditions.					
34420737	9	5	theme	MRM	1406:1408	arg1	method					1410:1415	the newly established LC-MS/MS MRM method	1375:1415	the newly established LC-MS/MS MRM method	1375:1415	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	9	5	theme	MRM	1406:1408	arg1	effective					1425:1433	effective	1425:1433	effective	1425:1433	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	4	6	theme	composition	679:689	arg1	characterization					644:659	the complete characterization	631:659	the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides	631:789	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	8	7	theme	developed	1318:1326	arg1	method					1328:1333	the developed method	1314:1333	the developed method	1314:1333	Seem bud can be successfully characterized using the developed method.					
34420737	9	8	theme	polysaccharides	1563:1577	arg1	analysis					1528:1535	the analysis	1524:1535	the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins	1524:1635	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	2	9	theme	liquid	291:296	arg1	GLC					314:316	GLC	314:316	GLC	314:316	Aldoses are commonly analyzed by gas liquid chromatography (GLC) following aldononitrile acetate derivatization (AND).					
34420737	2	9	theme	liquid	291:296	arg1	chromatography					298:311	gas liquid chromatography	287:311	gas liquid chromatography (GLC) following aldononitrile acetate derivatization (AND)	287:370	Aldoses are commonly analyzed by gas liquid chromatography (GLC) following aldononitrile acetate derivatization (AND).					
34420737	5	10	theme	LC	917:918	arg1	conditions					920:929	LC conditions	917:929	LC conditions	917:929	In addition to discussing the possible byproducts, the study optimizes the multiple reaction monitoring (MRM) parameters and LC conditions.					
34420737	4	11	theme	mass	577:580	arg1	LC-MS/MS					596:603	LC-MS/MS	596:603	LC-MS/MS	596:603	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	11	theme	mass	577:580	arg1	spectrometry					582:593	liquid chromatography-tandem mass spectrometry	548:593	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	548:604	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	12	theme	amino	726:730	arg1	sugars					732:737	amino sugars	726:737	amino sugars	726:737	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	13	dep	composition	679:689	arg1	alditols					716:723	alditols	716:723	alditols	716:723	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	13	dep	composition	679:689	arg1	sugars					732:737	amino sugars	726:737	amino sugars	726:737	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	13	dep	composition	679:689	arg1	ketoses					707:713	ketoses	707:713	ketoses	707:713	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	13	dep	composition	679:689	arg1	aldoses					698:704	aldoses	698:704	aldoses	698:704	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	13	dep	composition	679:689	arg1	acids					751:755	uronic acids	744:755	uronic acids	744:755	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	2	14	theme	gas	287:289	arg1	GLC					314:316	GLC	314:316	GLC	314:316	Aldoses are commonly analyzed by gas liquid chromatography (GLC) following aldononitrile acetate derivatization (AND).					
34420737	2	14	theme	gas	287:289	arg1	chromatography					298:311	gas liquid chromatography	287:311	gas liquid chromatography (GLC) following aldononitrile acetate derivatization (AND)	287:370	Aldoses are commonly analyzed by gas liquid chromatography (GLC) following aldononitrile acetate derivatization (AND).					
34420737	4	15	from	characterization	644:659	arg1	polysaccharides					775:789	plant-derived polysaccharides	761:789	plant-derived polysaccharides	761:789	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	16	theme	chromatography-tandem	555:575	arg1	LC-MS/MS					596:603	LC-MS/MS	596:603	LC-MS/MS	596:603	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	16	theme	chromatography-tandem	555:575	arg1	spectrometry					582:593	liquid chromatography-tandem mass spectrometry	548:593	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	548:604	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	0	17	theme	reaction	115:122	arg1	monitoring					124:133	multiple reaction monitoring	106:133	multiple reaction monitoring	106:133	A novel LC-MS/MS method for complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring.					
34420737	5	18	theme	reaction	876:883	arg1	parameters					902:911	the multiple reaction monitoring (MRM) parameters	863:911	the multiple reaction monitoring (MRM) parameters	863:911	In addition to discussing the possible byproducts, the study optimizes the multiple reaction monitoring (MRM) parameters and LC conditions.					
34420737	6	19	theme	RP18	1002:1005	arg1	column					1007:1012	a BEH Shield RP18 column	989:1012	a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm)	989:1038	The final separation of 17 carbohydrates is performed on a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm) within 25 min, without using any buffer salt.					
34420737	6	20	dep	column	1007:1012	arg1	150 mm × 2.1 mm					1015:1029	150 mm × 2.1 mm	1015:1029	150 mm × 2.1 mm	1015:1029	The final separation of 17 carbohydrates is performed on a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm) within 25 min, without using any buffer salt.					
34420737	6	20	dep	column	1007:1012	arg1	1.7 μm					1032:1037	1.7 μm	1032:1037	1.7 μm	1032:1037	The final separation of 17 carbohydrates is performed on a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm) within 25 min, without using any buffer salt.					
34420737	9	21	theme	LC-MS/MS	1397:1404	arg1	method					1410:1415	the newly established LC-MS/MS MRM method	1375:1415	the newly established LC-MS/MS MRM method	1375:1415	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	9	21	theme	LC-MS/MS	1397:1404	arg1	effective					1425:1433	effective	1425:1433	effective	1425:1433	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	1	22	theme	challenging	176:186	arg1	analysis					149:156	Carbohydrate analysis	136:156	Carbohydrate analysis	136:156	Carbohydrate analysis has always been a challenging task due to the occurrence of high polarity and multiple isomers.					
34420737	1	22	theme	challenging	176:186	arg1	task					188:191	a challenging task	174:191	a challenging task due to the occurrence of high polarity and multiple isomers	174:251	Carbohydrate analysis has always been a challenging task due to the occurrence of high polarity and multiple isomers.					
34420737	0	23	theme	multiple	106:113	arg1	monitoring					124:133	multiple reaction monitoring	106:133	multiple reaction monitoring	106:133	A novel LC-MS/MS method for complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring.					
34420737	6	24	theme	carbohydrates	959:971	arg1	separation					942:951	The final separation	932:951	The final separation of 17 carbohydrates	932:971	The final separation of 17 carbohydrates is performed on a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm) within 25 min, without using any buffer salt.					
34420737	4	25	theme	spectrometry	582:593	arg1	combination					533:543	the combination	529:543	the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS)	529:604	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	9	26	theme	established	1385:1395	arg1	method					1410:1415	the newly established LC-MS/MS MRM method	1375:1415	the newly established LC-MS/MS MRM method	1375:1415	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	9	26	theme	established	1385:1395	arg1	effective					1425:1433	effective	1425:1433	effective	1425:1433	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	0	27	theme	novel	2:6	arg1	method					17:22	A novel LC-MS/MS method	0:22	A novel LC-MS/MS method for complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring.	0:134	A novel LC-MS/MS method for complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring.					
34420737	9	28	theme	glycoproteins	1623:1635	arg1	analysis					1528:1535	the analysis	1524:1535	the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins	1524:1635	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	1	29	theme	due	193:195	arg1	analysis					149:156	Carbohydrate analysis	136:156	Carbohydrate analysis	136:156	Carbohydrate analysis has always been a challenging task due to the occurrence of high polarity and multiple isomers.					
34420737	1	29	theme	due	193:195	arg1	task					188:191	a challenging task	174:191	a challenging task due to the occurrence of high polarity and multiple isomers	174:251	Carbohydrate analysis has always been a challenging task due to the occurrence of high polarity and multiple isomers.					
34420737	3	30	theme	GLC	386:388	arg1	technique					390:398	the GLC technique	382:398	the GLC technique	382:398	However, the GLC technique cannot be applied for the simultaneous determination of aldoses, ketoses, and uronic acids.					
34420737	6	31	theme	final	936:940	arg1	separation					942:951	The final separation	932:951	The final separation of 17 carbohydrates	932:971	The final separation of 17 carbohydrates is performed on a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm) within 25 min, without using any buffer salt.					
34420737	5	32	theme	possible	822:829	arg1	byproducts					831:840	the possible byproducts	818:840	the possible byproducts	818:840	In addition to discussing the possible byproducts, the study optimizes the multiple reaction monitoring (MRM) parameters and LC conditions.					
34420737	4	33	dep	aldoses	698:704	arg1	i.e.					692:695	i.e.	692:695	i.e.	692:695	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	4	34	link	plant-derived	761:773	arg1	polysaccharides					775:789	plant-derived polysaccharides	761:789	plant-derived polysaccharides	761:789	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	0	35	theme	LC-MS/MS	8:15	arg1	method					17:22	A novel LC-MS/MS method	0:22	A novel LC-MS/MS method for complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring.	0:134	A novel LC-MS/MS method for complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring.					
34420737	5	36	theme	multiple	867:874	arg1	parameters					902:911	the multiple reaction monitoring (MRM) parameters	863:911	the multiple reaction monitoring (MRM) parameters	863:911	In addition to discussing the possible byproducts, the study optimizes the multiple reaction monitoring (MRM) parameters and LC conditions.					
34420737	3	37	theme	ketoses	465:471	arg1	determination					439:451	the simultaneous determination	422:451	the simultaneous determination of aldoses, ketoses, and uronic acids	422:489	However, the GLC technique cannot be applied for the simultaneous determination of aldoses, ketoses, and uronic acids.					
34420737	0	38	theme	complete	28:35	arg1	analysis					49:56	complete composition analysis	28:56	complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring	28:133	A novel LC-MS/MS method for complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring.					
34420737	3	39	theme	acids	485:489	arg1	determination					439:451	the simultaneous determination	422:451	the simultaneous determination of aldoses, ketoses, and uronic acids	422:489	However, the GLC technique cannot be applied for the simultaneous determination of aldoses, ketoses, and uronic acids.					
34420737	9	40	gly	glycoproteins	1623:1635	arg1	glycoproteins					1623:1635	glycoproteins	1623:1635	glycoproteins	1623:1635	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	9	40	gly	glycoproteins	1623:1635	arg1	pectins					1598:1604	complex pectins	1590:1604	complex pectins	1590:1604	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	4	41	theme	complete	635:642	arg1	characterization					644:659	the complete characterization	631:659	the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides	631:789	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	6	42	theme	Shield	995:1000	arg1	column					1007:1012	a BEH Shield RP18 column	989:1012	a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm)	989:1038	The final separation of 17 carbohydrates is performed on a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm) within 25 min, without using any buffer salt.					
34420737	3	43	theme	simultaneous	426:437	arg1	determination					439:451	the simultaneous determination	422:451	the simultaneous determination of aldoses, ketoses, and uronic acids	422:489	However, the GLC technique cannot be applied for the simultaneous determination of aldoses, ketoses, and uronic acids.					
34420737	4	44	theme	plant-derived	761:773	arg1	polysaccharides					775:789	plant-derived polysaccharides	761:789	plant-derived polysaccharides	761:789	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	1	45	theme	high	218:221	arg1	polarity					223:230	high polarity	218:230	high polarity	218:230	Carbohydrate analysis has always been a challenging task due to the occurrence of high polarity and multiple isomers.					
34420737	6	46	theme	BEH	991:993	arg1	column					1007:1012	a BEH Shield RP18 column	989:1012	a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm)	989:1038	The final separation of 17 carbohydrates is performed on a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm) within 25 min, without using any buffer salt.					
34420737	3	47	theme	uronic	478:483	arg1	acids					485:489	uronic acids	478:489	uronic acids	478:489	However, the GLC technique cannot be applied for the simultaneous determination of aldoses, ketoses, and uronic acids.					
34420737	0	48	theme	composition	37:47	arg1	analysis					49:56	complete composition analysis	28:56	complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring	28:133	A novel LC-MS/MS method for complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring.					
34420737	9	49	theme	complex	1547:1553	arg1	polysaccharides					1563:1577	highly complex natural polysaccharides	1540:1577	highly complex natural polysaccharides	1540:1577	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	9	49	theme	complex	1547:1553	arg1	pectins					1598:1604	complex pectins	1590:1604	complex pectins	1590:1604	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	4	50	from	polysaccharides	775:789	arg1	characterization					644:659	the complete characterization	631:659	the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides	631:789	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	2	51	theme	acetate	343:349	arg1	derivatization					351:364	aldononitrile acetate derivatization	329:364	aldononitrile acetate derivatization	329:364	Aldoses are commonly analyzed by gas liquid chromatography (GLC) following aldononitrile acetate derivatization (AND).					
34420737	4	52	theme	new	509:511	arg1	method					513:518	a new method	507:518	a new method	507:518	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	7	53	dep	chuanxiong	1149:1158	arg1	elata					1251:1255	elata	1251:1255	elata	1251:1255	Notably, the complex polysaccharides extracted from Ligusticum chuanxiong, Platycodon grandiflorum, Cyathula officinalis Kuan, Juglans mandshurica Maxim, and Aralia elata (Miq.)					
34420737	9	54	theme	natural	1555:1561	arg1	polysaccharides					1563:1577	highly complex natural polysaccharides	1540:1577	highly complex natural polysaccharides	1540:1577	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	9	54	theme	natural	1555:1561	arg1	pectins					1598:1604	complex pectins	1590:1604	complex pectins	1590:1604	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	5	55	dep	discussing	807:816	arg1	addition					795:802	addition	795:802	addition	795:802	In addition to discussing the possible byproducts, the study optimizes the multiple reaction monitoring (MRM) parameters and LC conditions.					
34420737	1	56	theme	Carbohydrate	136:147	arg1	analysis					149:156	Carbohydrate analysis	136:156	Carbohydrate analysis	136:156	Carbohydrate analysis has always been a challenging task due to the occurrence of high polarity and multiple isomers.					
34420737	1	56	theme	Carbohydrate	136:147	arg1	task					188:191	a challenging task	174:191	a challenging task due to the occurrence of high polarity and multiple isomers	174:251	Carbohydrate analysis has always been a challenging task due to the occurrence of high polarity and multiple isomers.					
34420737	1	57	theme	polarity	223:230	arg1	occurrence					204:213	the occurrence	200:213	the occurrence of high polarity and multiple isomers	200:251	Carbohydrate analysis has always been a challenging task due to the occurrence of high polarity and multiple isomers.					
34420737	9	58	theme	fructosans	1607:1616	arg1	analysis					1528:1535	the analysis	1524:1535	the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins	1524:1635	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	4	59	theme	uronic	744:749	arg1	acids					751:755	uronic acids	744:755	uronic acids	744:755	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	5	60	theme	MRM	897:899	arg1	parameters					902:911	the multiple reaction monitoring (MRM) parameters	863:911	the multiple reaction monitoring (MRM) parameters	863:911	In addition to discussing the possible byproducts, the study optimizes the multiple reaction monitoring (MRM) parameters and LC conditions.					
34420737	6	61	theme	buffer	1073:1078	arg1	salt					1080:1083	any buffer salt	1069:1083	any buffer salt	1069:1083	The final separation of 17 carbohydrates is performed on a BEH Shield RP18 column (150 mm × 2.1 mm, 1.7 μm) within 25 min, without using any buffer salt.					
34420737	2	62	theme	aldononitrile	329:341	arg1	derivatization					351:364	aldononitrile acetate derivatization	329:364	aldononitrile acetate derivatization	329:364	Aldoses are commonly analyzed by gas liquid chromatography (GLC) following aldononitrile acetate derivatization (AND).					
34420737	0	63	theme	polysaccharides	61:75	arg1	analysis					49:56	complete composition analysis	28:56	complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring	28:133	A novel LC-MS/MS method for complete composition analysis of polysaccharides by aldononitrile acetate and multiple reaction monitoring.					
34420737	3	64	theme	aldoses	456:462	arg1	determination					439:451	the simultaneous determination	422:451	the simultaneous determination of aldoses, ketoses, and uronic acids	422:489	However, the GLC technique cannot be applied for the simultaneous determination of aldoses, ketoses, and uronic acids.					
34420737	9	65	theme	GLC-based	1457:1465	arg1	methods					1467:1473	the GLC-based methods	1453:1473	the GLC-based methods reported previously	1453:1493	Overall, the results demonstrated that the newly established LC-MS/MS MRM method is more effective and powerful than the GLC-based methods reported previously, and it is more suitable for the analysis of highly complex natural polysaccharides, including complex pectins, fructosans, and glycoproteins.					
34420737	4	66	theme	monosaccharide	664:677	arg1	composition					679:689	monosaccharide composition	664:689	monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides	664:789	In this study, a new method based on the combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and AND is developed for the complete characterization of monosaccharide composition (i.e., aldoses, ketoses, alditols, amino sugars, and uronic acids) in plant-derived polysaccharides.					
34420737	1	67	theme	multiple	236:243	arg1	isomers					245:251	multiple isomers	236:251	multiple isomers	236:251	Carbohydrate analysis has always been a challenging task due to the occurrence of high polarity and multiple isomers.					
34420737	7	68	theme	complex	1099:1105	arg1	polysaccharides					1107:1121	the complex polysaccharides	1095:1121	the complex polysaccharides	1095:1121	Notably, the complex polysaccharides extracted from Ligusticum chuanxiong, Platycodon grandiflorum, Cyathula officinalis Kuan, Juglans mandshurica Maxim, and Aralia elata (Miq.)					
34420737	1	69	theme	isomers	245:251	arg1	occurrence					204:213	the occurrence	200:213	the occurrence of high polarity and multiple isomers	200:251	Carbohydrate analysis has always been a challenging task due to the occurrence of high polarity and multiple isomers.					
33910719	4	0	theme	tail	589:592	arg1	vein					594:597	mouse tail vein	583:597	mouse tail vein	583:597	Furthermore, the hemostasis experiments on mouse tail vein and rabbit femoral artery were also performed in vivo.					
33910719	1	1	theme	Schiff	185:190	arg1	reaction					210:217	Schiff base crosslinking reaction	185:217	Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose	185:285	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	4	2	theme	rabbit	603:608	arg1	artery					618:623	rabbit femoral artery	603:623	rabbit femoral artery	603:623	Furthermore, the hemostasis experiments on mouse tail vein and rabbit femoral artery were also performed in vivo.					
33910719	5	3	theme	excellent	771:779	arg1	quality					797:803	excellent water-absorbing quality	771:803	excellent water-absorbing quality	771:803	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	1	4	theme	base	192:195	arg1	reaction					210:217	Schiff base crosslinking reaction	185:217	Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose	185:285	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	6	5	theme	coagulation	1138:1148	arg1	candidate					1182:1190	a good candidate	1175:1190	a good candidate for emergency trauma treatment in daily civilian and military hemostasis	1175:1263	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	6	5	theme	coagulation	1138:1148	arg1	pathway					1150:1156	the endogenous coagulation pathway	1123:1156	the endogenous coagulation pathway	1123:1156	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	5	6	theme	water-absorbing	781:795	arg1	quality					797:803	excellent water-absorbing quality	771:803	excellent water-absorbing quality	771:803	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	5	7	theme	such	690:693	arg1	sponges					740:746	such synergistic cellulose-modified chitosan foam sponges	690:746	such synergistic cellulose-modified chitosan foam sponges	690:746	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	2	8	theme	electron	347:354	arg1	microscopy					356:365	scanning electron microscopy	338:365	scanning electron microscopy	338:365	Such composite foam sponges were characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy to confirm their morphology and compositions.					
33910719	6	9	theme	endogenous	1127:1136	arg1	candidate					1182:1190	a good candidate	1175:1190	a good candidate for emergency trauma treatment in daily civilian and military hemostasis	1175:1263	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	6	9	theme	endogenous	1127:1136	arg1	pathway					1150:1156	the endogenous coagulation pathway	1123:1156	the endogenous coagulation pathway	1123:1156	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	5	10	theme	benign	860:865	arg1	cytotoxicity					867:878	benign cytotoxicity	860:878	benign cytotoxicity	860:878	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	5	11	theme	synergistic	695:705	arg1	sponges					740:746	such synergistic cellulose-modified chitosan foam sponges	690:746	such synergistic cellulose-modified chitosan foam sponges	690:746	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	2	12	theme	scanning	338:345	arg1	microscopy					356:365	scanning electron microscopy	338:365	scanning electron microscopy	338:365	Such composite foam sponges were characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy to confirm their morphology and compositions.					
33910719	4	13	theme	hemostasis	557:566	arg1	experiments					568:578	the hemostasis experiments	553:578	the hemostasis experiments on mouse tail vein and rabbit femoral artery	553:623	Furthermore, the hemostasis experiments on mouse tail vein and rabbit femoral artery were also performed in vivo.					
33910719	5	14	theme	chitosan	726:733	arg1	sponges					740:746	such synergistic cellulose-modified chitosan foam sponges	690:746	such synergistic cellulose-modified chitosan foam sponges	690:746	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	5	15	theme	cellulose-modified	707:724	arg1	sponges					740:746	such synergistic cellulose-modified chitosan foam sponges	690:746	such synergistic cellulose-modified chitosan foam sponges	690:746	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	1	16	theme	Serial	81:86	arg1	sponges					99:105	Serial hemostatic sponges	81:105	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges	81:166	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	1	17	theme	crosslinking	197:208	arg1	reaction					210:217	Schiff base crosslinking reaction	185:217	Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose	185:285	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	4	18	theme	mouse	583:587	arg1	vein					594:597	mouse tail vein	583:597	mouse tail vein	583:597	Furthermore, the hemostasis experiments on mouse tail vein and rabbit femoral artery were also performed in vivo.					
33910719	5	19	theme	foam	735:738	arg1	sponges					740:746	such synergistic cellulose-modified chitosan foam sponges	690:746	such synergistic cellulose-modified chitosan foam sponges	690:746	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	1	20	theme	hemostatic	88:97	arg1	sponges					99:105	Serial hemostatic sponges	81:105	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges	81:166	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	6	21	theme	daily	1226:1230	arg1	civilian					1232:1239	daily civilian and military hemostasis	1226:1263	civilian	1232:1239	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	3	22	theme	coagulation	466:476	arg1	process					478:484	Then the coagulation process	457:484	Then the coagulation process	457:484	Then the coagulation process was evaluated in vitro by thrombus elasticity meters.					
33910719	4	23	from	experiments	568:578	arg1	vein					594:597	mouse tail vein	583:597	mouse tail vein	583:597	Furthermore, the hemostasis experiments on mouse tail vein and rabbit femoral artery were also performed in vivo.					
33910719	4	23	from	experiments	568:578	arg1	artery					618:623	rabbit femoral artery	603:623	rabbit femoral artery	603:623	Furthermore, the hemostasis experiments on mouse tail vein and rabbit femoral artery were also performed in vivo.					
33910719	2	24	theme	infrared	389:396	arg1	spectroscopy					398:409	Fourier-transform infrared spectroscopy	371:409	Fourier-transform infrared spectroscopy	371:409	Such composite foam sponges were characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy to confirm their morphology and compositions.					
33910719	6	25	theme	trauma	1206:1211	arg1	treatment					1213:1221	emergency trauma treatment	1196:1221	emergency trauma treatment in daily civilian and military hemostasis	1196:1263	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	5	26	theme	hemostasis	946:955	arg1	capability					957:966	superior hemostasis capability	937:966	superior hemostasis capability	937:966	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	6	27	theme	blood	1068:1072	arg1	cell/platelet					1074:1086	the red blood cell/platelet	1060:1086	the red blood cell/platelet	1060:1086	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	2	28	theme	Fourier-transform	371:387	arg1	spectroscopy					398:409	Fourier-transform infrared spectroscopy	371:409	Fourier-transform infrared spectroscopy	371:409	Such composite foam sponges were characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy to confirm their morphology and compositions.					
33910719	3	29	theme	thrombus	512:519	arg1	meters					532:537	thrombus elasticity meters	512:537	thrombus elasticity meters	512:537	Then the coagulation process was evaluated in vitro by thrombus elasticity meters.					
33910719	0	30	theme	improved	37:44	arg1	sponges					72:78	improved and rapid hemostasis foam sponges	37:78	improved and rapid hemostasis foam sponges	37:78	Polysaccharides-modified chitosan as improved and rapid hemostasis foam sponges.					
33910719	0	30	theme	improved	37:44	arg1	chitosan					25:32	chitosan	25:32	chitosan	25:32	Polysaccharides-modified chitosan as improved and rapid hemostasis foam sponges.					
33910719	6	31	from	treatment	1213:1221	arg1	civilian					1232:1239	daily civilian and military hemostasis	1226:1263	civilian	1232:1239	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	6	31	from	treatment	1213:1221	arg1	hemostasis					1254:1263	daily civilian and military hemostasis	1226:1263	hemostasis	1254:1263	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	1	32	theme	deacetylated	231:242	arg1	chitosan					244:251	chitosan	244:251	chitosan	244:251	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	6	33	theme	blood	1103:1107	arg1	clots					1109:1113	robust blood clots	1096:1113	robust blood clots	1096:1113	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	5	34	theme	low	839:841	arg1	rates					853:857	low hemolysis rates	839:857	low hemolysis rates	839:857	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	1	35	theme	polysaccharides-modified	121:144	arg1	sponges					160:166	polysaccharides-modified chitosan foam sponges	121:166	polysaccharides-modified chitosan foam sponges	121:166	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	0	36	theme	rapid	50:54	arg1	sponges					72:78	improved and rapid hemostasis foam sponges	37:78	improved and rapid hemostasis foam sponges	37:78	Polysaccharides-modified chitosan as improved and rapid hemostasis foam sponges.					
33910719	0	36	theme	rapid	50:54	arg1	chitosan					25:32	chitosan	25:32	chitosan	25:32	Polysaccharides-modified chitosan as improved and rapid hemostasis foam sponges.					
33910719	6	37	theme	robust	1096:1101	arg1	clots					1109:1113	robust blood clots	1096:1113	robust blood clots	1096:1113	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	5	38	theme	hemolysis	843:851	arg1	rates					853:857	low hemolysis rates	839:857	low hemolysis rates	839:857	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	2	39	theme	Such	288:291	arg1	sponges					308:314	Such composite foam sponges	288:314	Such composite foam sponges	288:314	Such composite foam sponges were characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy to confirm their morphology and compositions.					
33910719	6	40	theme	emergency	1196:1204	arg1	treatment					1213:1221	emergency trauma treatment	1196:1221	emergency trauma treatment in daily civilian and military hemostasis	1196:1263	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	6	41	theme	good	1177:1180	arg1	candidate					1182:1190	a good candidate	1175:1190	a good candidate for emergency trauma treatment in daily civilian and military hemostasis	1175:1263	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	6	41	theme	good	1177:1180	arg1	pathway					1150:1156	the endogenous coagulation pathway	1123:1156	the endogenous coagulation pathway	1123:1156	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	3	42	theme	elasticity	521:530	arg1	meters					532:537	thrombus elasticity meters	512:537	thrombus elasticity meters	512:537	Then the coagulation process was evaluated in vitro by thrombus elasticity meters.					
33910719	1	43	theme	oxidized	257:264	arg1	cellulose					277:285	oxidized dialdehyde cellulose	257:285	oxidized dialdehyde cellulose	257:285	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	0	44	theme	foam	67:70	arg1	sponges					72:78	improved and rapid hemostasis foam sponges	37:78	improved and rapid hemostasis foam sponges	37:78	Polysaccharides-modified chitosan as improved and rapid hemostasis foam sponges.					
33910719	0	44	theme	foam	67:70	arg1	chitosan					25:32	chitosan	25:32	chitosan	25:32	Polysaccharides-modified chitosan as improved and rapid hemostasis foam sponges.					
33910719	4	45	theme	femoral	610:616	arg1	artery					618:623	rabbit femoral artery	603:623	rabbit femoral artery	603:623	Furthermore, the hemostasis experiments on mouse tail vein and rabbit femoral artery were also performed in vivo.					
33910719	6	46	theme	hemostatic	1012:1021	arg1	mechanism					1023:1031	the hemostatic mechanism	1008:1031	the hemostatic mechanism	1008:1031	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	6	46	theme	hemostatic	1012:1021	arg1	adhering/activating					1040:1058	adhering/activating	1040:1058	adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis	1040:1263	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	1	47	theme	chitosan	146:153	arg1	sponges					160:166	polysaccharides-modified chitosan foam sponges	121:166	polysaccharides-modified chitosan foam sponges	121:166	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	0	48	theme	hemostasis	56:65	arg1	sponges					72:78	improved and rapid hemostasis foam sponges	37:78	improved and rapid hemostasis foam sponges	37:78	Polysaccharides-modified chitosan as improved and rapid hemostasis foam sponges.					
33910719	0	48	theme	hemostasis	56:65	arg1	chitosan					25:32	chitosan	25:32	chitosan	25:32	Polysaccharides-modified chitosan as improved and rapid hemostasis foam sponges.					
33910719	5	49	theme	improved	806:813	arg1	performance					826:836	improved mechanical performance	806:836	improved mechanical performance	806:836	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	1	50	theme	foam	155:158	arg1	sponges					160:166	polysaccharides-modified chitosan foam sponges	121:166	polysaccharides-modified chitosan foam sponges	121:166	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	2	51	theme	composite	293:301	arg1	sponges					308:314	Such composite foam sponges	288:314	Such composite foam sponges	288:314	Such composite foam sponges were characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy to confirm their morphology and compositions.					
33910719	5	52	theme	mechanical	815:824	arg1	performance					826:836	improved mechanical performance	806:836	improved mechanical performance	806:836	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	1	53	theme	dialdehyde	266:275	arg1	cellulose					277:285	oxidized dialdehyde cellulose	257:285	oxidized dialdehyde cellulose	257:285	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	5	54	theme	repeated	911:918	arg1	compression					920:930	repeated compression	911:930	repeated compression	911:930	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	5	55	theme	good	881:884	arg1	ability					897:903	good resilience ability	881:903	good resilience ability after repeated compression	881:930	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	2	56	theme	foam	303:306	arg1	sponges					308:314	Such composite foam sponges	288:314	Such composite foam sponges	288:314	Such composite foam sponges were characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy to confirm their morphology and compositions.					
33910719	6	57	theme	military	1245:1252	arg1	hemostasis					1254:1263	daily civilian and military hemostasis	1226:1263	hemostasis	1254:1263	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	5	58	theme	resilience	886:895	arg1	ability					897:903	good resilience ability	881:903	good resilience ability after repeated compression	881:930	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33910719	6	59	theme	red	1064:1066	arg1	cell/platelet					1074:1086	the red blood cell/platelet	1060:1086	the red blood cell/platelet	1060:1086	Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.					
33910719	1	60	dep	chitosan	244:251	arg1	the					227:229	the	227:229	the	227:229	Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose.					
33910719	5	61	theme	superior	937:944	arg1	capability					957:966	superior hemostasis capability	937:966	superior hemostasis capability	937:966	The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo.					
33098893	2	0	theme	nuclear	467:473	arg1	NMR					495:497	NMR	495:497	NMR	495:497	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	2	0	theme	nuclear	467:473	arg1	resonance					484:492	nuclear magnetic resonance	467:492	nuclear magnetic resonance (NMR) spectroscopy	467:511	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	5	1	theme	inhibitory	773:782	arg1	activities					784:793	optimal inhibitory activities	765:793	optimal inhibitory activities	765:793	Seven H-8s exhibited optimal inhibitory activities, which varied based on different sources of Danshen.					
33098893	4	2	theme	least	715:719	arg1	analysis					728:735	partial least square analysis	707:735	partial least square analysis (PLS)	707:741	Monosaccharides difference and correlation were performed by partial least square analysis (PLS).					
33098893	4	2	theme	least	715:719	arg1	PLS					738:740	PLS	738:740	PLS	738:740	Monosaccharides difference and correlation were performed by partial least square analysis (PLS).					
33098893	7	3	from	variation	1056:1064	arg1	terms					1077:1081	terms	1077:1081	terms of the structure and bioactivity of SMRs correlated with different cultivation sites	1077:1166	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	7	4	theme	SMRs	1119:1122	arg1	bioactivity					1104:1114	bioactivity	1104:1114	bioactivity	1104:1114	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	7	4	theme	SMRs	1119:1122	arg1	structure					1090:1098	structure	1090:1098	structure	1090:1098	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	1	5	theme	cell	170:173	arg1	wall					175:178	cell wall	170:178	cell wall from the residual waste extraction	170:213	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	2	6	from	solution	313:320	arg1	extraction					277:286	The extraction	273:286	The extraction from 8% sodium hydroxide solution (H-8)	273:326	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	3	7	theme	structure	518:526	arg1	model					528:532	The structure model	514:532	The structure model of hemicellulose-based polysaccharides (HBPs)	514:578	The structure model of hemicellulose-based polysaccharides (HBPs) was derived by combining one-dimensional and two-dimensional NMR.					
33098893	3	8	theme	hemicellulose-based	537:555	arg1	HBPs					574:577	HBPs	574:577	HBPs	574:577	The structure model of hemicellulose-based polysaccharides (HBPs) was derived by combining one-dimensional and two-dimensional NMR.					
33098893	3	8	theme	hemicellulose-based	537:555	arg1	polysaccharides					557:571	hemicellulose-based polysaccharides	537:571	hemicellulose-based polysaccharides (HBPs)	537:578	The structure model of hemicellulose-based polysaccharides (HBPs) was derived by combining one-dimensional and two-dimensional NMR.					
33098893	1	9	theme	monosaccharides	256:270	arg1	ways					248:251	the forming ways	236:251	the forming ways of monosaccharides	236:270	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	3	10	theme	polysaccharides	557:571	arg1	model					528:532	The structure model	514:532	The structure model of hemicellulose-based polysaccharides (HBPs)	514:578	The structure model of hemicellulose-based polysaccharides (HBPs) was derived by combining one-dimensional and two-dimensional NMR.					
33098893	6	11	theme	main	911:914	arg1	chain					916:920	the main chain	907:920	the main chain connected by 3-α-L-Araf or 5-α-L-Araf-1, 4-β-D-Galp, and β-D-Glcp branch, as well as α-L-Rhap, α-D-GalpA and α-D-GlcpA fragments	907:1049	The backbone structure indicated that 4-β-D-Xylp served as the main chain connected by 3-α-L-Araf or 5-α-L-Araf-1, 4-β-D-Galp, and β-D-Glcp branch, as well as α-L-Rhap, α-D-GalpA and α-D-GlcpA fragments.					
33098893	6	11	theme	main	911:914	arg1	4-β-D-Xylp					886:895	4-β-D-Xylp	886:895	4-β-D-Xylp	886:895	The backbone structure indicated that 4-β-D-Xylp served as the main chain connected by 3-α-L-Araf or 5-α-L-Araf-1, 4-β-D-Galp, and β-D-Glcp branch, as well as α-L-Rhap, α-D-GalpA and α-D-GlcpA fragments.					
33098893	7	12	theme	bioactivity	1104:1114	arg1	terms					1077:1081	terms	1077:1081	terms of the structure and bioactivity of SMRs correlated with different cultivation sites	1077:1166	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	7	13	theme	different	1140:1148	arg1	sites					1162:1166	different cultivation sites	1140:1166	different cultivation sites	1140:1166	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	4	14	theme	partial	707:713	arg1	analysis					728:735	partial least square analysis	707:735	partial least square analysis (PLS)	707:741	Monosaccharides difference and correlation were performed by partial least square analysis (PLS).					
33098893	4	14	theme	partial	707:713	arg1	PLS					738:740	PLS	738:740	PLS	738:740	Monosaccharides difference and correlation were performed by partial least square analysis (PLS).					
33098893	7	15	theme	chemical	1239:1246	arg1	aspects					1263:1269	chemical and biological aspects	1239:1269	chemical and biological aspects of natural macromolecules	1239:1295	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	2	16	dep	transform	422:430	arg1	infrared					432:439	infrared	432:439	transform infrared spectroscopy (FT-IR)	422:460	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	1	17	from	component	157:165	arg1	extraction					204:213	the residual waste extraction	185:213	the residual waste extraction	185:213	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	1	18	theme	wall	175:178	arg1	component					157:165	the main component	148:165	the main component of cell wall from the residual waste extraction	148:213	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	1	18	theme	wall	175:178	arg1	roots					134:138	Salvia miltiorrhiza roots	114:138	Salvia miltiorrhiza roots (SMRs)	114:145	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	7	19	dep	structure	1090:1098	arg1	the					1086:1088	the	1086:1088	the	1086:1088	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	2	20	dep	Fourier	414:420	arg1	transform					422:430	transform	422:430	transform infrared spectroscopy (FT-IR)	422:460	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	5	21	theme	different	818:826	arg1	sources					828:834	different sources	818:834	different sources of Danshen	818:845	Seven H-8s exhibited optimal inhibitory activities, which varied based on different sources of Danshen.					
33098893	7	22	theme	new	1177:1179	arg1	approach					1181:1188	a new approach	1175:1188	a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules	1175:1295	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	7	22	theme	new	1177:1179	arg1	variation					1056:1064	The variation	1052:1064	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites	1052:1166	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	1	23	theme	Salvia	114:119	arg1	component					157:165	the main component	148:165	the main component of cell wall from the residual waste extraction	148:213	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	1	23	theme	Salvia	114:119	arg1	SMRs					141:144	SMRs	141:144	SMRs	141:144	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	1	23	theme	Salvia	114:119	arg1	roots					134:138	Salvia miltiorrhiza roots	114:138	Salvia miltiorrhiza roots (SMRs)	114:145	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	0	24	theme	Chemical	0:7	arg1	characteristic					9:22	Chemical characteristic	0:22	Chemical characteristic	0:22	Chemical characteristic and bioactivity of hemicellulose-based polysaccharides isolated from Salvia miltiorrhiza.					
33098893	2	25	theme	hydroxide	303:311	arg1	H-8					323:325	H-8	323:325	H-8	323:325	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	2	25	theme	hydroxide	303:311	arg1	solution					313:320	8% sodium hydroxide solution	293:320	8% sodium hydroxide solution (H-8)	293:326	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	1	26	theme	residual	189:196	arg1	extraction					204:213	the residual waste extraction	185:213	the residual waste extraction	185:213	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	6	27	theme	β-D-Glcp	979:986	arg1	branch					988:993	β-D-Glcp branch	979:993	β-D-Glcp branch	979:993	The backbone structure indicated that 4-β-D-Xylp served as the main chain connected by 3-α-L-Araf or 5-α-L-Araf-1, 4-β-D-Galp, and β-D-Glcp branch, as well as α-L-Rhap, α-D-GalpA and α-D-GlcpA fragments.					
33098893	7	28	theme	HBPs	1069:1072	arg1	approach					1181:1188	a new approach	1175:1188	a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules	1175:1295	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	7	28	theme	HBPs	1069:1072	arg1	variation					1056:1064	The variation	1052:1064	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites	1052:1166	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	2	29	theme	sodium	296:301	arg1	H-8					323:325	H-8	323:325	H-8	323:325	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	2	29	theme	sodium	296:301	arg1	solution					313:320	8% sodium hydroxide solution	293:320	8% sodium hydroxide solution (H-8)	293:326	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	1	30	theme	waste	198:202	arg1	extraction					204:213	the residual waste extraction	185:213	the residual waste extraction	185:213	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	1	31	theme	miltiorrhiza	121:132	arg1	component					157:165	the main component	148:165	the main component of cell wall from the residual waste extraction	148:213	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	1	31	theme	miltiorrhiza	121:132	arg1	SMRs					141:144	SMRs	141:144	SMRs	141:144	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	1	31	theme	miltiorrhiza	121:132	arg1	roots					134:138	Salvia miltiorrhiza roots	114:138	Salvia miltiorrhiza roots (SMRs)	114:145	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	2	32	theme	%	294:294	arg1	H-8					323:325	H-8	323:325	H-8	323:325	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	2	32	theme	%	294:294	arg1	solution					313:320	8% sodium hydroxide solution	293:320	8% sodium hydroxide solution (H-8)	293:326	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	1	33	theme	main	152:155	arg1	component					157:165	the main component	148:165	the main component of cell wall from the residual waste extraction	148:213	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	1	33	theme	main	152:155	arg1	roots					134:138	Salvia miltiorrhiza roots	114:138	Salvia miltiorrhiza roots (SMRs)	114:145	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	0	34	theme	hemicellulose-based	43:61	arg1	polysaccharides					63:77	hemicellulose-based polysaccharides	43:77	hemicellulose-based polysaccharides	43:77	Chemical characteristic and bioactivity of hemicellulose-based polysaccharides isolated from Salvia miltiorrhiza.					
33098893	7	35	theme	natural	1274:1280	arg1	macromolecules					1282:1295	natural macromolecules	1274:1295	natural macromolecules	1274:1295	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	7	36	theme	macromolecules	1282:1295	arg1	aspects					1263:1269	chemical and biological aspects	1239:1269	chemical and biological aspects of natural macromolecules	1239:1295	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	4	37	dep	Monosaccharides	646:660	arg1	Monosaccharides					646:660	Monosaccharides difference and correlation	646:687	Monosaccharides difference and correlation	646:687	Monosaccharides difference and correlation were performed by partial least square analysis (PLS).					
33098893	4	37	dep	Monosaccharides	646:660	arg1	correlation					677:687	correlation	677:687	correlation	677:687	Monosaccharides difference and correlation were performed by partial least square analysis (PLS).					
33098893	4	37	dep	Monosaccharides	646:660	arg1	difference					662:671	difference	662:671	difference	662:671	Monosaccharides difference and correlation were performed by partial least square analysis (PLS).					
33098893	2	38	theme	monosaccharide	386:399	arg1	composition					401:411	monosaccharide composition	386:411	monosaccharide composition	386:411	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	7	39	theme	cultivation	1150:1160	arg1	sites					1162:1166	different cultivation sites	1140:1166	different cultivation sites	1140:1166	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	3	40	theme	two-dimensional	625:639	arg1	NMR					641:643	one-dimensional and two-dimensional NMR	605:643	one-dimensional and two-dimensional NMR	605:643	The structure model of hemicellulose-based polysaccharides (HBPs) was derived by combining one-dimensional and two-dimensional NMR.					
33098893	6	41	theme	α-D-GlcpA	1031:1039	arg1	fragments					1041:1049	α-D-GlcpA fragments	1031:1049	α-D-GlcpA fragments	1031:1049	The backbone structure indicated that 4-β-D-Xylp served as the main chain connected by 3-α-L-Araf or 5-α-L-Araf-1, 4-β-D-Galp, and β-D-Glcp branch, as well as α-L-Rhap, α-D-GalpA and α-D-GlcpA fragments.					
33098893	5	42	theme	optimal	765:771	arg1	activities					784:793	optimal inhibitory activities	765:793	optimal inhibitory activities	765:793	Seven H-8s exhibited optimal inhibitory activities, which varied based on different sources of Danshen.					
33098893	0	43	theme	polysaccharides	63:77	arg1	bioactivity					28:38	bioactivity	28:38	bioactivity	28:38	Chemical characteristic and bioactivity of hemicellulose-based polysaccharides isolated from Salvia miltiorrhiza.					
33098893	0	43	theme	polysaccharides	63:77	arg1	characteristic					9:22	Chemical characteristic	0:22	Chemical characteristic	0:22	Chemical characteristic and bioactivity of hemicellulose-based polysaccharides isolated from Salvia miltiorrhiza.					
33098893	6	44	theme	backbone	852:859	arg1	structure					861:869	The backbone structure	848:869	The backbone structure	848:869	The backbone structure indicated that 4-β-D-Xylp served as the main chain connected by 3-α-L-Araf or 5-α-L-Araf-1, 4-β-D-Galp, and β-D-Glcp branch, as well as α-L-Rhap, α-D-GalpA and α-D-GlcpA fragments.					
33098893	7	45	theme	structure	1090:1098	arg1	terms					1077:1081	terms	1077:1081	terms of the structure and bioactivity of SMRs correlated with different cultivation sites	1077:1166	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	4	46	theme	square	721:726	arg1	analysis					728:735	partial least square analysis	707:735	partial least square analysis (PLS)	707:741	Monosaccharides difference and correlation were performed by partial least square analysis (PLS).					
33098893	4	46	theme	square	721:726	arg1	PLS					738:740	PLS	738:740	PLS	738:740	Monosaccharides difference and correlation were performed by partial least square analysis (PLS).					
33098893	3	47	theme	one-dimensional	605:619	arg1	NMR					641:643	one-dimensional and two-dimensional NMR	605:643	one-dimensional and two-dimensional NMR	605:643	The structure model of hemicellulose-based polysaccharides (HBPs) was derived by combining one-dimensional and two-dimensional NMR.					
33098893	1	48	from	extraction	204:213	arg1	component					157:165	the main component	148:165	the main component of cell wall from the residual waste extraction	148:213	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	1	48	from	extraction	204:213	arg1	wall					175:178	cell wall	170:178	cell wall from the residual waste extraction	170:213	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	1	48	from	extraction	204:213	arg1	roots					134:138	Salvia miltiorrhiza roots	114:138	Salvia miltiorrhiza roots (SMRs)	114:145	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
33098893	7	49	theme	biological	1252:1261	arg1	aspects					1263:1269	chemical and biological aspects	1239:1269	chemical and biological aspects of natural macromolecules	1239:1295	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	2	50	theme	resonance	484:492	arg1	spectroscopy					500:511	nuclear magnetic resonance (NMR) spectroscopy	467:511	nuclear magnetic resonance (NMR) spectroscopy	467:511	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	5	51	theme	Danshen	839:845	arg1	sources					828:834	different sources	818:834	different sources of Danshen	818:845	Seven H-8s exhibited optimal inhibitory activities, which varied based on different sources of Danshen.					
33098893	2	52	theme	permeation	353:362	arg1	GPC					380:382	GPC	380:382	GPC	380:382	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	2	52	theme	permeation	353:362	arg1	chromatography					364:377	gel permeation chromatography	349:377	gel permeation chromatography (GPC)	349:383	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	7	53	theme	medical	1218:1224	arg1	materials					1226:1234	the medical materials	1214:1234	the medical materials	1214:1234	The variation of HBPs in terms of the structure and bioactivity of SMRs correlated with different cultivation sites can be a new approach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules.					
33098893	2	54	theme	magnetic	475:482	arg1	NMR					495:497	NMR	495:497	NMR	495:497	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	2	54	theme	magnetic	475:482	arg1	resonance					484:492	nuclear magnetic resonance	467:492	nuclear magnetic resonance (NMR) spectroscopy	467:511	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	2	55	theme	gel	349:351	arg1	GPC					380:382	GPC	380:382	GPC	380:382	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	2	55	theme	gel	349:351	arg1	chromatography					364:377	gel permeation chromatography	349:377	gel permeation chromatography (GPC)	349:383	The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy.					
33098893	1	56	theme	forming	240:246	arg1	ways					248:251	the forming ways	236:251	the forming ways of monosaccharides	236:270	Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides.					
32756606	0	0	theme	proteins	84:91	arg1	interaction					103:113	their interaction	97:113	their interaction with the SARS-CoV-2 spike protein binding domain	97:162	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	0	0	theme	proteins	84:91	arg1	models					59:64	fully glycosylated molecular models	30:64	fully glycosylated molecular models of ACE2-Fc fusion proteins	30:91	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	4	1	theme	glycosylation	668:680	arg1	modelling					682:690	protein glycosylation modelling	660:690	protein glycosylation modelling in order to expedite future model development	660:736	Additionally, we optimize algorithms for protein glycosylation modelling in order to expedite future model development.					
32756606	0	2	theme	fusion	77:82	arg1	proteins					84:91	ACE2-Fc fusion proteins	69:91	ACE2-Fc fusion proteins	69:91	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	3	3	theme	ACE2	604:607	arg1	receptor					609:616	the ACE2 receptor	600:616	the ACE2 receptor	600:616	We see that some ACE2 glycans interact with the S fragments, and glycans are influencing the conformation of the ACE2 receptor.					
32756606	1	4	gly	glycosylated	182:193	arg1	targets					263:269	promising targets	253:269	promising targets for a COVID-19 therapeutic	253:296	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	1	4	gly	glycosylated	182:193	arg1	models					209:214	fully glycosylated computational models	176:214	fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic	176:296	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	0	5	theme	interaction	103:113	arg1	simulation					16:25	simulation	16:25	simulation	16:25	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	0	5	theme	interaction	103:113	arg1	Development					0:10	Development	0:10	Development	0:10	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	2	6	theme	molecular	459:467	arg1	simulations					478:488	atomistic molecular dynamics simulations	449:488	atomistic molecular dynamics simulations	449:488	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	2	7	theme	virus	438:442	arg1	S					418:418	the Spike Protein S	400:418	the Spike Protein S of the SARS-CoV-2 virus	400:442	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	2	8	theme	dynamics	469:476	arg1	simulations					478:488	atomistic molecular dynamics simulations	449:488	atomistic molecular dynamics simulations	449:488	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	0	9	with	interaction	103:113	arg1	domain					157:162	the SARS-CoV-2 spike protein binding domain	120:162	the SARS-CoV-2 spike protein binding domain	120:162	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	1	10	theme	glycosylated	182:193	arg1	targets					263:269	promising targets	253:269	promising targets for a COVID-19 therapeutic	253:296	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	1	10	theme	glycosylated	182:193	arg1	models					209:214	fully glycosylated computational models	176:214	fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic	176:296	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	2	11	theme	SARS-CoV-2	427:436	arg1	virus					438:442	the SARS-CoV-2 virus	423:442	the SARS-CoV-2 virus	423:442	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	4	12	theme	model	720:724	arg1	development					726:736	future model development	713:736	future model development	713:736	Additionally, we optimize algorithms for protein glycosylation modelling in order to expedite future model development.					
32756606	1	13	theme	computational	195:207	arg1	targets					263:269	promising targets	253:269	promising targets for a COVID-19 therapeutic	253:296	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	1	13	theme	computational	195:207	arg1	models					209:214	fully glycosylated computational models	176:214	fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic	176:296	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	0	14	theme	SARS-CoV-2	124:133	arg1	domain					157:162	the SARS-CoV-2 spike protein binding domain	120:162	the SARS-CoV-2 spike protein binding domain	120:162	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	3	15	theme	S	539:539	arg1	fragments					541:549	the S fragments	535:549	the S fragments	535:549	We see that some ACE2 glycans interact with the S fragments, and glycans are influencing the conformation of the ACE2 receptor.					
32756606	2	16	theme	S	418:418	arg1	S					418:418	the Spike Protein S	400:418	the Spike Protein S of the SARS-CoV-2 virus	400:442	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	2	16	theme	S	418:418	arg1	RBD					392:394	RBD	392:394	RBD	392:394	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	2	16	theme	S	418:418	arg1	domain					384:389	the receptor-binding domain	363:389	the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus	363:442	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	2	17	with	interaction	332:342	arg1	fragment					351:358	a fragment	349:358	a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus	349:442	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	2	18	theme	Protein	410:416	arg1	S					418:418	the Spike Protein S	400:418	the Spike Protein S of the SARS-CoV-2 virus	400:442	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	0	19	theme	glycosylated	36:47	arg1	models					59:64	fully glycosylated molecular models	30:64	fully glycosylated molecular models of ACE2-Fc fusion proteins	30:91	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	0	20	theme	protein	141:147	arg1	domain					157:162	the SARS-CoV-2 spike protein binding domain	120:162	the SARS-CoV-2 spike protein binding domain	120:162	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	2	21	theme	Spike	404:408	arg1	S					418:418	the Spike Protein S	400:418	the Spike Protein S of the SARS-CoV-2 virus	400:442	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	1	22	theme	ACE2-Fc	219:225	arg1	proteins					234:241	ACE2-Fc fusion proteins	219:241	ACE2-Fc fusion proteins	219:241	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	2	23	theme	atomistic	449:457	arg1	simulations					478:488	atomistic molecular dynamics simulations	449:488	atomistic molecular dynamics simulations	449:488	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	0	24	theme	spike	135:139	arg1	domain					157:162	the SARS-CoV-2 spike protein binding domain	120:162	the SARS-CoV-2 spike protein binding domain	120:162	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	3	25	theme	receptor	609:616	arg1	conformation					584:595	the conformation	580:595	the conformation of the ACE2 receptor	580:616	We see that some ACE2 glycans interact with the S fragments, and glycans are influencing the conformation of the ACE2 receptor.					
32756606	1	26	theme	fusion	227:232	arg1	proteins					234:241	ACE2-Fc fusion proteins	219:241	ACE2-Fc fusion proteins	219:241	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	0	27	theme	models	59:64	arg1	simulation					16:25	simulation	16:25	simulation	16:25	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	0	27	theme	models	59:64	arg1	Development					0:10	Development	0:10	Development	0:10	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	0	28	theme	molecular	49:57	arg1	models					59:64	fully glycosylated molecular models	30:64	fully glycosylated molecular models of ACE2-Fc fusion proteins	30:91	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	0	29	theme	binding	149:155	arg1	domain					157:162	the SARS-CoV-2 spike protein binding domain	120:162	the SARS-CoV-2 spike protein binding domain	120:162	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	4	30	theme	protein	660:666	arg1	modelling					682:690	protein glycosylation modelling	660:690	protein glycosylation modelling in order to expedite future model development	660:736	Additionally, we optimize algorithms for protein glycosylation modelling in order to expedite future model development.					
32756606	4	31	theme	future	713:718	arg1	development					726:736	future model development	713:736	future model development	713:736	Additionally, we optimize algorithms for protein glycosylation modelling in order to expedite future model development.					
32756606	1	32	theme	proteins	234:241	arg1	targets					263:269	promising targets	253:269	promising targets for a COVID-19 therapeutic	253:296	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	1	32	theme	proteins	234:241	arg1	models					209:214	fully glycosylated computational models	176:214	fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic	176:296	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	0	33	gly	glycosylated	36:47	arg1	models					59:64	fully glycosylated molecular models	30:64	fully glycosylated molecular models of ACE2-Fc fusion proteins	30:91	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	0	34	with	models	59:64	arg1	domain					157:162	the SARS-CoV-2 spike protein binding domain	120:162	the SARS-CoV-2 spike protein binding domain	120:162	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	3	35	theme	ACE2	508:511	arg1	glycans					513:519	some ACE2 glycans	503:519	some ACE2 glycans	503:519	We see that some ACE2 glycans interact with the S fragments, and glycans are influencing the conformation of the ACE2 receptor.					
32756606	0	36	theme	ACE2-Fc	69:75	arg1	proteins					84:91	ACE2-Fc fusion proteins	69:91	ACE2-Fc fusion proteins	69:91	Development and simulation of fully glycosylated molecular models of ACE2-Fc fusion proteins and their interaction with the SARS-CoV-2 spike protein binding domain.					
32756606	2	37	theme	domain	384:389	arg1	fragment					351:358	a fragment	349:358	a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus	349:442	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	2	38	theme	receptor-binding	367:382	arg1	S					418:418	the Spike Protein S	400:418	the Spike Protein S of the SARS-CoV-2 virus	400:442	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	2	38	theme	receptor-binding	367:382	arg1	RBD					392:394	RBD	392:394	RBD	392:394	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	2	38	theme	receptor-binding	367:382	arg1	domain					384:389	the receptor-binding domain	363:389	the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus	363:442	These models are tested in their interaction with a fragment of the receptor-binding domain (RBD) of the Spike Protein S of the SARS-CoV-2 virus, via atomistic molecular dynamics simulations.					
32756606	1	39	theme	promising	253:261	arg1	models					209:214	fully glycosylated computational models	176:214	fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic	176:296	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32756606	1	39	theme	promising	253:261	arg1	targets					263:269	promising targets	253:269	promising targets for a COVID-19 therapeutic	253:296	We develop fully glycosylated computational models of ACE2-Fc fusion proteins which are promising targets for a COVID-19 therapeutic.					
32155332	7	0	theme	normal	1599:1604	arg1	individuals					1606:1616	normal individuals	1599:1616	normal individuals	1599:1616	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	4	1	theme	interactions	927:938	arg1	motions					875:881	concerted motions	865:881	concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region	865:982	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	6	2	theme	patients	1408:1415	arg1	sera					1377:1380	sera	1377:1380	sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC)	1377:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	3	3	theme	colonic	563:569	arg1	TRs					592:594	TRs	592:594	TRs	592:594	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	3	theme	colonic	563:569	arg1	repeats					583:589	colonic MUC2 tandem repeats	563:589	colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties	563:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	8	4	theme	epitopes	1809:1816	arg1	creation					1789:1796	the creation	1785:1796	the creation of dynamic epitopes	1785:1816	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	6	5	theme	autoantibodies	1307:1320	arg1	presence					1287:1294	the presence	1283:1294	the presence of natural autoantibodies to MUC2	1283:1328	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	3	6	from	motifs	685:690	arg1	O-glycosylation					662:676	O-glycosylation	662:676	O-glycosylation at TTX motifs	662:690	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	6	7	theme	volunteers	1393:1402	arg1	sera					1377:1380	sera	1377:1380	sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC)	1377:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	8	8	theme	immature	1821:1828	arg1	O-glycosylation					1830:1844	immature O-glycosylation	1821:1844	immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy	1821:1963	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	7	9	theme	early	1579:1583	arg1	CRC					1591:1593	early stage CRC	1579:1593	early stage CRC	1579:1593	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	4	10	theme	dihedral	895:902	arg1	angles					904:909	multiple dihedral angles	886:909	multiple dihedral angles	886:909	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	7	11	theme	patients	1534:1541	arg1	sera					1507:1510	sera	1507:1510	sera of the late stage CRC patients	1507:1541	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	2	12	theme	dynamic	313:319	arg1	epitopes					321:328	dynamic epitopes	313:328	dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs)	313:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	7	13	theme	stage	1524:1528	arg1	patients					1534:1541	the late stage CRC patients	1515:1541	the late stage CRC patients	1515:1541	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	2	14	theme	immature	364:371	arg1	O-glycosylation					373:387	aberrantly truncated immature O-glycosylation	343:387	aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs)	343:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	5	15	theme	restricted	1149:1158	arg1	orientations					1160:1171	restricted orientations	1149:1171	restricted orientations permitting further sugar extension	1149:1206	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	1	16	theme	promising	236:244	arg1	microenvironments					214:230	microenvironments	214:230	microenvironments	214:230	Autoantibody signatures of circulating mucin fragments stem from cancer tissues, and microenvironments are promising biomarkers for cancer diagnosis and therapy.					
32155332	1	16	theme	promising	236:244	arg1	biomarkers					246:255	promising biomarkers	236:255	promising biomarkers for cancer diagnosis and therapy	236:288	Autoantibody signatures of circulating mucin fragments stem from cancer tissues, and microenvironments are promising biomarkers for cancer diagnosis and therapy.					
32155332	8	17	gly	O-glycosylation	1830:1844	arg1	motifs					1853:1858	TTX motifs	1849:1858	TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy	1849:1963	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	8	17	gly	O-glycosylation	1830:1844	arg2	motifs					1853:1858	TTX motifs	1849:1858	TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy	1849:1963	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	3	18	theme	general	639:645	arg1	principle					647:655	a general principle	637:655	a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs	637:749	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	6	19	theme	stage	1438:1442	arg1	CRC					1463:1465	CRC	1463:1465	CRC	1463:1465	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	6	19	theme	stage	1438:1442	arg1	cancer					1455:1460	early stage colorectal cancer	1432:1460	early stage colorectal cancer (CRC)	1432:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	6	20	theme	impaired	1335:1342	arg1	O-glycosylation					1344:1358	impaired O-glycosylation	1335:1358	impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC)	1335:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	3	21	gly	glycosylated	535:546	arg1	motifs					552:557	glycosylated TTX motifs	535:557	glycosylated TTX motifs	535:557	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	8	22	theme	high-potential	1909:1922	arg1	targets					1924:1930	high-potential targets	1909:1930	high-potential targets for cancer diagnosis and therapy	1909:1963	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	1	23	theme	mucin	168:172	arg1	fragments					174:182	circulating mucin fragments	156:182	circulating mucin fragments	156:182	Autoantibody signatures of circulating mucin fragments stem from cancer tissues, and microenvironments are promising biomarkers for cancer diagnosis and therapy.					
32155332	7	24	theme	CRC	1716:1718	arg1	stage					1720:1724	the CRC stage	1712:1724	the CRC stage of patients	1712:1736	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	8	25	theme	dynamic	1801:1807	arg1	epitopes					1809:1816	dynamic epitopes	1801:1816	dynamic epitopes	1801:1816	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	2	26	located	found	427:431	arg1	proteins					472:479	intrinsically disordered proteins	447:479	intrinsically disordered proteins (IDPs)	447:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	2	26	located	found	427:431	arg2	TTX					422:424	TTX	422:424	TTX	422:424	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	2	26	located	found	427:431	arg2	motifs					414:419	consecutive threonine motifs	392:419	consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs)	392:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	2	26	located	found	427:431	arg1	mucins					436:441	mucins	436:441	mucins	436:441	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	2	26	located	found	427:431	arg1	IDPs					482:485	IDPs	482:485	IDPs	482:485	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	0	27	theme	Conformationally	81:96	arg1	Neoepitopes					116:126	Conformationally Restricted Cancer Neoepitopes	81:126	Conformationally Restricted Cancer Neoepitopes	81:126	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.					
32155332	1	28	theme	cancer	194:199	arg1	tissues					201:207	cancer tissues	194:207	cancer tissues	194:207	Autoantibody signatures of circulating mucin fragments stem from cancer tissues, and microenvironments are promising biomarkers for cancer diagnosis and therapy.					
32155332	4	29	theme	motifs	818:823	arg1	conformation					785:796	the specific conformation	772:796	the specific conformation of glycosylated TTX motifs in MUC2 TRs	772:835	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	1	30	theme	Autoantibody	129:140	arg1	signatures					142:151	Autoantibody signatures	129:151	Autoantibody signatures of circulating mucin fragments	129:182	Autoantibody signatures of circulating mucin fragments stem from cancer tissues, and microenvironments are promising biomarkers for cancer diagnosis and therapy.					
32155332	0	31	theme	Cancer	109:114	arg1	Neoepitopes					116:126	Conformationally Restricted Cancer Neoepitopes	81:126	Conformationally Restricted Cancer Neoepitopes	81:126	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.					
32155332	5	32	theme	sugar	1192:1196	arg1	extension					1198:1206	further sugar extension	1184:1206	further sugar extension	1184:1206	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	4	33	from	motifs	818:823	arg1	TRs					833:835	MUC2 TRs	828:835	MUC2 TRs	828:835	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	5	34	theme	motifs	1046:1051	arg1	conformation					1013:1024	this canonical conformation	998:1024	this canonical conformation of glycosylated TTX motifs	998:1051	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	7	35	theme	MUC2	1669:1672	arg1	neoepitopes					1674:1684	MUC2 neoepitopes	1669:1684	MUC2 neoepitopes	1669:1684	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	5	36	gly	glycosylated	1029:1040	arg1	motifs					1046:1051	glycosylated TTX motifs	1029:1051	glycosylated TTX motifs	1029:1051	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	0	37	from	Motifs	54:59	arg1	O-Glycosylation					9:23	Impaired O-Glycosylation	0:23	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins	0:69	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.					
32155332	4	38	theme	MUC2	828:831	arg1	TRs					833:835	MUC2 TRs	828:835	MUC2 TRs	828:835	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	3	39	theme	NMR	489:491	arg1	analysis					493:500	NMR analysis	489:500	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties	489:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	4	40	gly	glycosylated	801:812	arg1	motifs					818:823	glycosylated TTX motifs	801:823	glycosylated TTX motifs in MUC2 TRs	801:835	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	5	41	theme	steric	1063:1068	arg1	crowding					1070:1077	steric crowding	1063:1077	steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension	1063:1206	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	0	42	theme	Impaired	0:7	arg1	O-Glycosylation					9:23	Impaired O-Glycosylation	0:23	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins	0:69	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.					
32155332	4	43	from	conformation	785:796	arg1	TRs					833:835	MUC2 TRs	828:835	MUC2 TRs	828:835	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	7	44	from	levels	1497:1502	arg1	sera					1507:1510	sera	1507:1510	sera of the late stage CRC patients	1507:1541	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	3	45	contain	containing	597:606	arg1	TRs					592:594	TRs	592:594	TRs	592:594	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	45	contain	containing	597:606	arg2	moieties					620:627	TTL moieties	616:627	TTL moieties	616:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	45	contain	containing	597:606	arg1	repeats					583:589	colonic MUC2 tandem repeats	563:589	colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties	563:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	45	contain	containing	597:606	arg2	TTP					608:610	TTP	608:610	TTP	608:610	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	4	46	theme	glycosylated	801:812	arg1	motifs					818:823	glycosylated TTX motifs	801:823	glycosylated TTX motifs in MUC2 TRs	801:835	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	3	47	theme	mucin	515:519	arg1	models					521:526	synthetic mucin models	505:526	synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties	505:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	7	48	theme	humoral	1649:1655	arg1	response					1657:1664	the anti-MUC2 humoral response	1635:1664	the anti-MUC2 humoral response to MUC2 neoepitopes	1635:1684	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	5	49	theme	glycosylated	1029:1040	arg1	motifs					1046:1051	glycosylated TTX motifs	1029:1051	glycosylated TTX motifs	1029:1051	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	5	50	contain	possess	1141:1147	arg1	O-glycans					1131:1139	O-glycans	1131:1139	O-glycans	1131:1139	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	5	50	contain	possess	1141:1147	arg2	orientations					1160:1171	restricted orientations	1149:1171	restricted orientations permitting further sugar extension	1149:1206	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	4	51	theme	concerted	865:873	arg1	motions					875:881	concerted motions	865:881	concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region	865:982	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	0	52	theme	Threonine	40:48	arg1	Motifs					54:59	Consecutive Threonine TTX Motifs	28:59	Consecutive Threonine TTX Motifs	28:59	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.					
32155332	5	53	theme	threonine	1102:1110	arg1	residues					1112:1119	threonine residues	1102:1119	threonine residues	1102:1119	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	3	54	theme	TTX	548:550	arg1	motifs					552:557	glycosylated TTX motifs	535:557	glycosylated TTX motifs	535:557	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	55	theme	rigid	724:728	arg1	conformation					730:741	a highly extended and rigid conformation	702:741	a highly extended and rigid conformation in IDPs	702:749	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	0	56	gly	O-Glycosylation	9:23	arg2	Motifs					54:59	Consecutive Threonine TTX Motifs	28:59	Consecutive Threonine TTX Motifs	28:59	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.					
32155332	2	57	theme	consecutive	392:402	arg1	TTX					422:424	TTX	422:424	TTX	422:424	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	2	57	theme	consecutive	392:402	arg1	motifs					414:419	consecutive threonine motifs	392:419	consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs)	392:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	8	58	theme	structural	1765:1774	arg1	basis					1776:1780	the structural basis	1761:1780	the structural basis of the creation of dynamic epitopes	1761:1816	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	1	59	theme	cancer	261:266	arg1	diagnosis					268:276	cancer diagnosis	261:276	cancer diagnosis	261:276	Autoantibody signatures of circulating mucin fragments stem from cancer tissues, and microenvironments are promising biomarkers for cancer diagnosis and therapy.					
32155332	6	60	theme	antiadhesive	1212:1223	arg1	microarray					1225:1234	An antiadhesive microarray	1209:1234	An antiadhesive microarray displaying synthetic MUC2 derivatives	1209:1272	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	2	61	theme	disordered	461:470	arg1	proteins					472:479	intrinsically disordered proteins	447:479	intrinsically disordered proteins (IDPs)	447:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	2	61	theme	disordered	461:470	arg1	IDPs					482:485	IDPs	482:485	IDPs	482:485	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	8	62	from	motifs	1853:1858	arg1	O-glycosylation					1830:1844	immature O-glycosylation	1821:1844	immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy	1821:1963	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	8	62	from	motifs	1853:1858	arg1	mucins					1863:1868	mucins	1863:1868	mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy	1863:1963	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	3	63	theme	MUC2	571:574	arg1	TRs					592:594	TRs	592:594	TRs	592:594	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	63	theme	MUC2	571:574	arg1	repeats					583:589	colonic MUC2 tandem repeats	563:589	colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties	563:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	8	64	theme	creation	1789:1796	arg1	basis					1776:1780	the structural basis	1761:1780	the structural basis of the creation of dynamic epitopes	1761:1816	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	7	65	theme	stage	1585:1589	arg1	CRC					1591:1593	early stage CRC	1579:1593	early stage CRC	1579:1593	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	4	66	theme	noncovalent	915:925	arg1	interactions					927:938	noncovalent interactions	915:938	noncovalent interactions between the carbohydrate and peptide region	915:982	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	8	67	theme	targets	1924:1930	arg1	identification					1891:1904	the identification	1887:1904	the identification of high-potential targets for cancer diagnosis and therapy	1887:1963	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	2	68	from	motifs	414:419	arg1	O-glycosylation					373:387	aberrantly truncated immature O-glycosylation	343:387	aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs)	343:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	6	69	theme	natural	1299:1305	arg1	autoantibodies					1307:1320	natural autoantibodies	1299:1320	natural autoantibodies to MUC2	1299:1328	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	6	70	gly	O-glycosylation	1344:1358	arg2	motifs					1367:1372	TTX motifs	1363:1372	TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC)	1363:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	6	70	gly	O-glycosylation	1344:1358	arg1	motifs					1367:1372	TTX motifs	1363:1372	TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC)	1363:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	6	71	theme	healthy	1385:1391	arg1	volunteers					1393:1402	healthy volunteers	1385:1402	healthy volunteers	1385:1402	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	4	72	theme	multiple	886:893	arg1	angles					904:909	multiple dihedral angles	886:909	multiple dihedral angles	886:909	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	3	73	gly	O-glycosylation	662:676	arg1	motifs					685:690	TTX motifs	681:690	TTX motifs	681:690	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	73	gly	O-glycosylation	662:676	arg2	motifs					685:690	TTX motifs	681:690	TTX motifs	681:690	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	4	74	theme	angles	904:909	arg1	motions					875:881	concerted motions	865:881	concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region	865:982	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	7	75	theme	CRC	1530:1532	arg1	patients					1534:1541	the late stage CRC patients	1515:1541	the late stage CRC patients	1515:1541	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	2	76	theme	truncated	354:362	arg1	O-glycosylation					373:387	aberrantly truncated immature O-glycosylation	343:387	aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs)	343:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	8	77	theme	TTX	1849:1851	arg1	motifs					1853:1858	TTX motifs	1849:1858	TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy	1849:1963	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	3	78	dep	principle	647:655	arg1	generates					692:700	generates	692:700	generates a highly extended and rigid conformation in IDPs	692:749	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	79	theme	TTL	616:618	arg1	moieties					620:627	TTL moieties	616:627	TTL moieties	616:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	6	80	theme	MUC2	1257:1260	arg1	derivatives					1262:1272	synthetic MUC2 derivatives	1247:1272	synthetic MUC2 derivatives	1247:1272	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	3	81	from	conformation	730:741	arg1	IDPs					746:749	IDPs	746:749	IDPs	746:749	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	7	82	theme	patients	1729:1736	arg1	stage					1720:1724	the CRC stage	1712:1724	the CRC stage of patients	1712:1736	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	6	83	theme	TTX	1363:1365	arg1	motifs					1367:1372	TTX motifs	1363:1372	TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC)	1363:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	6	84	theme	colorectal	1444:1453	arg1	CRC					1463:1465	CRC	1463:1465	CRC	1463:1465	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	6	84	theme	colorectal	1444:1453	arg1	cancer					1455:1460	early stage colorectal cancer	1432:1460	early stage colorectal cancer (CRC)	1432:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	5	85	theme	further	1184:1190	arg1	extension					1198:1206	further sugar extension	1184:1206	further sugar extension	1184:1206	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	4	86	theme	carbohydrate	952:963	arg1	region					977:982	the carbohydrate and peptide region	948:982	region	977:982	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	6	87	theme	early	1432:1436	arg1	CRC					1463:1465	CRC	1463:1465	CRC	1463:1465	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	6	87	theme	early	1432:1436	arg1	cancer					1455:1460	early stage colorectal cancer	1432:1460	early stage colorectal cancer (CRC)	1432:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	1	88	theme	circulating	156:166	arg1	fragments					174:182	circulating mucin fragments	156:182	circulating mucin fragments	156:182	Autoantibody signatures of circulating mucin fragments stem from cancer tissues, and microenvironments are promising biomarkers for cancer diagnosis and therapy.					
32155332	4	89	theme	peptide	969:975	arg1	region					977:982	the carbohydrate and peptide region	948:982	region	977:982	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	7	90	theme	autoantibody	1484:1495	arg1	levels					1497:1502	autoantibody levels	1484:1502	autoantibody levels in sera of the late stage CRC patients	1484:1541	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	6	91	from	motifs	1367:1372	arg1	sera					1377:1380	sera	1377:1380	sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC)	1377:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	6	91	from	motifs	1367:1372	arg1	O-glycosylation					1344:1358	impaired O-glycosylation	1335:1358	impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC)	1335:1466	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	1	92	theme	fragments	174:182	arg1	signatures					142:151	Autoantibody signatures	129:151	Autoantibody signatures of circulating mucin fragments	129:182	Autoantibody signatures of circulating mucin fragments stem from cancer tissues, and microenvironments are promising biomarkers for cancer diagnosis and therapy.					
32155332	0	93	theme	Restricted	98:107	arg1	Neoepitopes					116:126	Conformationally Restricted Cancer Neoepitopes	81:126	Conformationally Restricted Cancer Neoepitopes	81:126	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.					
32155332	4	94	from	TRs	833:835	arg1	conformation					785:796	the specific conformation	772:796	the specific conformation of glycosylated TTX motifs in MUC2 TRs	772:835	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	0	95	from	O-Glycosylation	9:23	arg1	Mucins					64:69	Mucins	64:69	Mucins	64:69	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.					
32155332	8	96	theme	cancer	1936:1941	arg1	diagnosis					1943:1951	cancer diagnosis	1936:1951	cancer diagnosis	1936:1951	Our results uncovered the structural basis of the creation of dynamic epitopes by immature O-glycosylation at TTX motifs in mucins that facilitates the identification of high-potential targets for cancer diagnosis and therapy.					
32155332	5	97	theme	TTX	1042:1044	arg1	motifs					1046:1051	glycosylated TTX motifs	1029:1051	glycosylated TTX motifs	1029:1051	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	7	98	theme	late	1519:1522	arg1	patients					1534:1541	the late stage CRC patients	1515:1541	the late stage CRC patients	1515:1541	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	5	99	attach	attached	1090:1097	arg1	residues					1112:1119	threonine residues	1102:1119	threonine residues	1102:1119	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	5	99	attach	attached	1090:1097	arg2	glycans					1082:1088	glycans	1082:1088	glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension	1082:1206	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	4	100	theme	TTX	814:816	arg1	motifs					818:823	glycosylated TTX motifs	801:823	glycosylated TTX motifs in MUC2 TRs	801:835	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	5	101	theme	canonical	1003:1011	arg1	conformation					1013:1024	this canonical conformation	998:1024	this canonical conformation of glycosylated TTX motifs	998:1051	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	3	102	contain	having	528:533	arg2	repeats					583:589	colonic MUC2 tandem repeats	563:589	colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties	563:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	102	contain	having	528:533	arg2	TRs					592:594	TRs	592:594	TRs	592:594	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	102	contain	having	528:533	arg2	motifs					552:557	glycosylated TTX motifs	535:557	glycosylated TTX motifs	535:557	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	102	contain	having	528:533	arg1	models					521:526	synthetic mucin models	505:526	synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties	505:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	103	theme	extended	711:718	arg1	conformation					730:741	a highly extended and rigid conformation	702:741	a highly extended and rigid conformation in IDPs	702:749	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	4	104	theme	specific	776:783	arg1	conformation					785:796	the specific conformation	772:796	the specific conformation of glycosylated TTX motifs in MUC2 TRs	772:835	We demonstrate that the specific conformation of glycosylated TTX motifs in MUC2 TRs is rationally rearranged by concerted motions of multiple dihedral angles and noncovalent interactions between the carbohydrate and peptide region.					
32155332	0	105	theme	Consecutive	28:38	arg1	Motifs					54:59	Consecutive Threonine TTX Motifs	28:59	Consecutive Threonine TTX Motifs	28:59	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.					
32155332	3	106	theme	models	521:526	arg1	analysis					493:500	NMR analysis	489:500	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties	489:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	0	107	theme	TTX	50:52	arg1	Motifs					54:59	Consecutive Threonine TTX Motifs	28:59	Consecutive Threonine TTX Motifs	28:59	Impaired O-Glycosylation at Consecutive Threonine TTX Motifs in Mucins Generates Conformationally Restricted Cancer Neoepitopes.					
32155332	3	108	theme	TTX	681:683	arg1	motifs					685:690	TTX motifs	681:690	TTX motifs	681:690	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	109	theme	synthetic	505:513	arg1	models					521:526	synthetic mucin models	505:526	synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties	505:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	7	110	theme	anti-MUC2	1639:1647	arg1	response					1657:1664	the anti-MUC2 humoral response	1635:1664	the anti-MUC2 humoral response to MUC2 neoepitopes	1635:1684	Interestingly, autoantibody levels in sera of the late stage CRC patients were distinctly lower than those of early stage CRC and normal individuals, indicating that the anti-MUC2 humoral response to MUC2 neoepitopes correlates inversely with the CRC stage of patients.					
32155332	6	111	theme	synthetic	1247:1255	arg1	derivatives					1262:1272	synthetic MUC2 derivatives	1247:1272	synthetic MUC2 derivatives	1247:1272	An antiadhesive microarray displaying synthetic MUC2 derivatives elicited the presence of natural autoantibodies to MUC2 with impaired O-glycosylation at TTX motifs in sera of healthy volunteers and patients diagnosed with early stage colorectal cancer (CRC).					
32155332	2	112	theme	threonine	404:412	arg1	TTX					422:424	TTX	422:424	TTX	422:424	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	2	112	theme	threonine	404:412	arg1	motifs					414:419	consecutive threonine motifs	392:419	consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs)	392:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	3	113	theme	glycosylated	535:546	arg1	motifs					552:557	glycosylated TTX motifs	535:557	glycosylated TTX motifs	535:557	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	5	114	theme	glycans	1082:1088	arg1	crowding					1070:1077	steric crowding	1063:1077	steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension	1063:1206	Importantly, this canonical conformation of glycosylated TTX motifs minimizes steric crowding of glycans attached to threonine residues, in which O-glycans possess restricted orientations permitting further sugar extension.					
32155332	2	115	gly	O-glycosylation	373:387	arg1	TTX					422:424	TTX	422:424	TTX	422:424	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	2	115	gly	O-glycosylation	373:387	arg2	TTX					422:424	TTX	422:424	TTX	422:424	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	2	115	gly	O-glycosylation	373:387	arg1	motifs					414:419	consecutive threonine motifs	392:419	consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs)	392:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	2	115	gly	O-glycosylation	373:387	arg2	motifs					414:419	consecutive threonine motifs	392:419	consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs)	392:486	This study highlights dynamic epitopes generated by aberrantly truncated immature O-glycosylation at consecutive threonine motifs (TTX) found in mucins and intrinsically disordered proteins (IDPs).					
32155332	3	116	theme	tandem	576:581	arg1	TRs					592:594	TRs	592:594	TRs	592:594	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
32155332	3	116	theme	tandem	576:581	arg1	repeats					583:589	colonic MUC2 tandem repeats	563:589	colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties	563:627	NMR analysis of synthetic mucin models having glycosylated TTX motifs and colonic MUC2 tandem repeats (TRs) containing TTP and TTL moieties unveils a general principle that O-glycosylation at TTX motifs generates a highly extended and rigid conformation in IDPs.					
33616012	0	0	from	analysis	9:16	arg1	properties					46:55	the carbohydrate-binding properties	21:55	the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla	21:105	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	9	1	theme	DlyL	1523:1526	arg1	activity					1541:1548	DlyL vasorelaxant activity	1523:1548	DlyL vasorelaxant activity	1523:1548	These results confirm the ability of DlyL to interact with high-mannose N-glycans with its expanded CRD, supporting the hypothesis that DlyL vasorelaxant activity occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma.					
33616012	5	2	theme	rat	833:835	arg1	rings					844:848	rat aortic rings	833:848	rat aortic rings	833:848	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	2	3	gly	glycoproteins	164:176	arg1	glycoproteins					164:176	glycoproteins	164:176	glycoproteins	164:176	Lectins are a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner.					
33616012	0	4	theme	lectin	75:80	arg1	properties					46:55	the carbohydrate-binding properties	21:55	the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla	21:105	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	3	5	attach	present	346:352	arg2	glycoconjugates					330:344	glycoconjugates	330:344	glycoconjugates present on the cell surface	330:372	Owing to this property, these proteins can interact with glycoconjugates present on the cell surface, making it possible to decipher the glycocode, as well as elicit biological effects, such as inflammation and vasorelaxation.					
33616012	3	5	attach	present	346:352	arg1	surface					366:372	the cell surface	357:372	the cell surface	357:372	Owing to this property, these proteins can interact with glycoconjugates present on the cell surface, making it possible to decipher the glycocode, as well as elicit biological effects, such as inflammation and vasorelaxation.					
33616012	8	6	with	rings	1253:1257	arg1	participation					1268:1280	the participation	1264:1280	the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO)	1264:1347	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	6	7	from	inputs	996:1001	arg1	simulations					1009:1019	MD simulations	1006:1019	MD simulations	1006:1019	In silico analysis of molecular docking was performed to obtain the initial coordinates of the DlyL complexes with the carbohydrates to apply as inputs in MD simulations.					
33616012	5	8	theme	aortic	837:842	arg1	rings					844:848	rat aortic rings	833:848	rat aortic rings	833:848	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	7	9	theme	interaction	1120:1130	arg1	profiles					1108:1115	different profiles	1098:1115	time as well as different profiles of interaction with Xman and N-glycans	1082:1154	The MD trajectories demonstrated the stability of DlyL over time as well as different profiles of interaction with Xman and N-glycans.					
33616012	7	9	theme	interaction	1120:1130	arg1	time					1082:1085	time	1082:1085	time as well as different profiles of interaction with Xman and N-glycans	1082:1154	The MD trajectories demonstrated the stability of DlyL over time as well as different profiles of interaction with Xman and N-glycans.					
33616012	7	10	with	interaction	1120:1130	arg1	N-glycans					1146:1154	N-glycans	1146:1154	N-glycans	1146:1154	The MD trajectories demonstrated the stability of DlyL over time as well as different profiles of interaction with Xman and N-glycans.					
33616012	7	10	with	interaction	1120:1130	arg1	Xman					1137:1140	Xman	1137:1140	Xman	1137:1140	The MD trajectories demonstrated the stability of DlyL over time as well as different profiles of interaction with Xman and N-glycans.					
33616012	4	11	theme	mannose/glucose-specific	557:580	arg1	lectin					582:587	the mannose/glucose-specific lectin	553:587	the mannose/glucose-specific lectin from Dioclea lasiophylla seeds	553:618	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	0	12	theme	Dioclea	87:93	arg1	lasiophylla					95:105	Dioclea lasiophylla	87:105	Dioclea lasiophylla	87:105	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	9	13	theme	DlyL	1424:1427	arg1	ability					1413:1419	the ability	1409:1419	the ability of DlyL to interact with high-mannose N-glycans with its expanded CRD	1409:1489	These results confirm the ability of DlyL to interact with high-mannose N-glycans with its expanded CRD, supporting the hypothesis that DlyL vasorelaxant activity occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma.					
33616012	9	14	theme	cell	1596:1599	arg1	surface					1601:1607	cell surface	1596:1607	cell surface	1596:1607	These results confirm the ability of DlyL to interact with high-mannose N-glycans with its expanded CRD, supporting the hypothesis that DlyL vasorelaxant activity occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma.					
33616012	0	15	dep	Mart	107:110	arg1	analysis					9:16	depth analysis	3:16	depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla	3:105	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	0	15	dep	Mart	107:110	arg1	Ex					112:113	Mart Ex	107:113	Mart Ex	107:113	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	6	16	dep	In	851:852	arg1	silico					854:859	silico	854:859	silico	854:859	In silico analysis of molecular docking was performed to obtain the initial coordinates of the DlyL complexes with the carbohydrates to apply as inputs in MD simulations.					
33616012	5	17	theme	high-mannose	703:714	arg1	N-glycans					716:724	high-mannose N-glycans	703:724	high-mannose N-glycans	703:724	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	0	18	from	properties	46:55	arg1	lasiophylla					95:105	Dioclea lasiophylla	87:105	Dioclea lasiophylla	87:105	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	3	19	dep	such	459:462	arg1	as					464:465	as	464:465	as	464:465	Owing to this property, these proteins can interact with glycoconjugates present on the cell surface, making it possible to decipher the glycocode, as well as elicit biological effects, such as inflammation and vasorelaxation.					
33616012	5	20	theme	in	793:794	arg1	effect					802:807	the resultant in vitro effect	779:807	the resultant in vitro effect on vasorelaxation using rat aortic rings	779:848	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	3	21	theme	present	346:352	arg1	glycoconjugates					330:344	glycoconjugates	330:344	glycoconjugates present on the cell surface	330:372	Owing to this property, these proteins can interact with glycoconjugates present on the cell surface, making it possible to decipher the glycocode, as well as elicit biological effects, such as inflammation and vasorelaxation.					
33616012	7	22	theme	MD	1026:1027	arg1	trajectories					1029:1040	The MD trajectories	1022:1040	The MD trajectories	1022:1040	The MD trajectories demonstrated the stability of DlyL over time as well as different profiles of interaction with Xman and N-glycans.					
33616012	6	23	theme	complexes	951:959	arg1	coordinates					927:937	the initial coordinates	915:937	the initial coordinates of the DlyL complexes with the carbohydrates	915:982	In silico analysis of molecular docking was performed to obtain the initial coordinates of the DlyL complexes with the carbohydrates to apply as inputs in MD simulations.					
33616012	9	24	with	interaction	1579:1589	arg1	surface					1601:1607	cell surface	1596:1607	cell surface	1596:1607	These results confirm the ability of DlyL to interact with high-mannose N-glycans with its expanded CRD, supporting the hypothesis that DlyL vasorelaxant activity occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma.					
33616012	0	25	theme	depth	3:7	arg1	analysis					9:16	depth analysis	3:16	depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla	3:105	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	5	26	from	dynamics	761:768	arg1	vasorelaxation					812:825	vasorelaxation	812:825	vasorelaxation using rat aortic rings	812:848	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	8	27	theme	domain	1314:1319	arg1	participation					1268:1280	the participation	1264:1280	the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO)	1264:1347	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	8	28	theme	rings	1177:1181	arg1	assays					1183:1188	aortic rings assays	1170:1188	aortic rings assays	1170:1188	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	7	29	theme	different	1098:1106	arg1	profiles					1108:1115	different profiles	1098:1115	time as well as different profiles of interaction with Xman and N-glycans	1082:1154	The MD trajectories demonstrated the stability of DlyL over time as well as different profiles of interaction with Xman and N-glycans.					
33616012	4	30	theme	structural	518:527	arg1	DlyL					621:624	DlyL	621:624	DlyL	621:624	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	4	30	theme	structural	518:527	arg1	study					544:548	a structural and biological study	516:548	a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds	516:618	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	9	31	dep	glycosylated	1609:1620	arg1	receptors.Communicated					1622:1643	receptors.Communicated	1622:1643	occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma	1550:1665	These results confirm the ability of DlyL to interact with high-mannose N-glycans with its expanded CRD, supporting the hypothesis that DlyL vasorelaxant activity occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma.					
33616012	8	32	theme	recognition	1302:1312	arg1	domain					1314:1319	the carbohydrate recognition domain	1285:1319	the carbohydrate recognition domain (CRD)	1285:1325	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	8	32	theme	recognition	1302:1312	arg1	CRD					1322:1324	CRD	1322:1324	CRD	1322:1324	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	8	33	theme	aortic	1170:1175	arg1	assays					1183:1188	aortic rings assays	1170:1188	aortic rings assays	1170:1188	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	3	34	theme	cell	361:364	arg1	surface					366:372	the cell surface	357:372	the cell surface	357:372	Owing to this property, these proteins can interact with glycoconjugates present on the cell surface, making it possible to decipher the glycocode, as well as elicit biological effects, such as inflammation and vasorelaxation.					
33616012	8	35	theme	nitric	1331:1336	arg1	NO					1345:1346	NO	1345:1346	NO	1345:1346	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	8	35	theme	nitric	1331:1336	arg1	oxide					1338:1342	nitric oxide	1331:1342	nitric oxide (NO)	1331:1347	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	4	36	theme	biological	533:542	arg1	DlyL					621:624	DlyL	621:624	DlyL	621:624	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	4	36	theme	biological	533:542	arg1	study					544:548	a structural and biological study	516:548	a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds	516:618	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	8	37	theme	carbohydrate	1289:1300	arg1	domain					1314:1319	the carbohydrate recognition domain	1285:1319	the carbohydrate recognition domain (CRD)	1285:1325	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	8	37	theme	carbohydrate	1289:1300	arg1	CRD					1322:1324	CRD	1322:1324	CRD	1322:1324	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	2	38	theme	glycoproteins	164:176	arg1	Lectins					129:135	Lectins	129:135	Lectins	129:135	Lectins are a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner.					
33616012	2	38	theme	glycoproteins	164:176	arg1	class					143:147	a class	141:147	a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner	141:270	Lectins are a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner.					
33616012	9	39	theme	high-mannose	1446:1457	arg1	N-glycans					1459:1467	high-mannose N-glycans	1446:1467	high-mannose N-glycans	1446:1467	These results confirm the ability of DlyL to interact with high-mannose N-glycans with its expanded CRD, supporting the hypothesis that DlyL vasorelaxant activity occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma.					
33616012	6	40	theme	In	851:852	arg1	analysis					861:868	In silico analysis	851:868	In silico analysis of molecular docking	851:889	In silico analysis of molecular docking was performed to obtain the initial coordinates of the DlyL complexes with the carbohydrates to apply as inputs in MD simulations.					
33616012	3	41	theme	biological	439:448	arg1	effects					450:456	biological effects	439:456	biological effects	439:456	Owing to this property, these proteins can interact with glycoconjugates present on the cell surface, making it possible to decipher the glycocode, as well as elicit biological effects, such as inflammation and vasorelaxation.					
33616012	0	42	theme	carbohydrate-binding	25:44	arg1	properties					46:55	the carbohydrate-binding properties	21:55	the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla	21:105	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	5	43	theme	DlyL	684:687	arg1	interaction					669:679	the interaction	665:679	the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings	665:848	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	8	44	theme	endothelial	1354:1364	arg1	tissue					1366:1371	endothelial tissue	1354:1371	endothelial tissue	1354:1371	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	4	45	from	seeds	614:618	arg1	DlyL					621:624	DlyL	621:624	DlyL	621:624	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	4	45	from	seeds	614:618	arg1	study					544:548	a structural and biological study	516:548	a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds	516:618	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	4	45	from	seeds	614:618	arg1	lectin					582:587	the mannose/glucose-specific lectin	553:587	the mannose/glucose-specific lectin from Dioclea lasiophylla seeds	553:618	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	5	46	with	interaction	669:679	arg1	Xman					694:697	Xman	694:697	Xman	694:697	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	5	46	with	interaction	669:679	arg1	N-glycans					716:724	high-mannose N-glycans	703:724	high-mannose N-glycans	703:724	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	6	47	theme	MD	1006:1007	arg1	simulations					1009:1019	MD simulations	1006:1019	MD simulations	1006:1019	In silico analysis of molecular docking was performed to obtain the initial coordinates of the DlyL complexes with the carbohydrates to apply as inputs in MD simulations.					
33616012	6	48	theme	docking	883:889	arg1	analysis					861:868	In silico analysis	851:868	In silico analysis of molecular docking	851:889	In silico analysis of molecular docking was performed to obtain the initial coordinates of the DlyL complexes with the carbohydrates to apply as inputs in MD simulations.					
33616012	2	49	theme	proteins	152:159	arg1	Lectins					129:135	Lectins	129:135	Lectins	129:135	Lectins are a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner.					
33616012	2	49	theme	proteins	152:159	arg1	class					143:147	a class	141:147	a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner	141:270	Lectins are a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner.					
33616012	6	50	theme	DlyL	946:949	arg1	complexes					951:959	the DlyL complexes	942:959	the DlyL complexes with the carbohydrates	942:982	In silico analysis of molecular docking was performed to obtain the initial coordinates of the DlyL complexes with the carbohydrates to apply as inputs in MD simulations.					
33616012	5	51	theme	resultant	783:791	arg1	effect					802:807	the resultant in vitro effect	779:807	the resultant in vitro effect on vasorelaxation using rat aortic rings	779:848	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	4	52	from	study	544:548	arg1	seeds					614:618	Dioclea lasiophylla seeds	594:618	Dioclea lasiophylla seeds	594:618	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	6	53	theme	molecular	873:881	arg1	docking					883:889	molecular docking	873:889	molecular docking	873:889	In silico analysis of molecular docking was performed to obtain the initial coordinates of the DlyL complexes with the carbohydrates to apply as inputs in MD simulations.					
33616012	5	54	dep	in	793:794	arg1	vitro					796:800	vitro	796:800	vitro	796:800	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	9	55	dep	occurs	1550:1555	arg1	glycosylated					1609:1620	glycosylated	1609:1620	occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma	1550:1665	These results confirm the ability of DlyL to interact with high-mannose N-glycans with its expanded CRD, supporting the hypothesis that DlyL vasorelaxant activity occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma.					
33616012	7	56	theme	DlyL	1072:1075	arg1	stability					1059:1067	the stability	1055:1067	the stability of DlyL	1055:1075	The MD trajectories demonstrated the stability of DlyL over time as well as different profiles of interaction with Xman and N-glycans.					
33616012	8	57	theme	oxide	1338:1342	arg1	participation					1268:1280	the participation	1264:1280	the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO)	1264:1347	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	6	58	with	complexes	951:959	arg1	carbohydrates					970:982	the carbohydrates	966:982	the carbohydrates	966:982	In silico analysis of molecular docking was performed to obtain the initial coordinates of the DlyL complexes with the carbohydrates to apply as inputs in MD simulations.					
33616012	1	59	theme	Benth	116:120	arg1	seeds					122:126	Benth seeds	116:126	Benth seeds.	116:127	Benth seeds.					
33616012	5	60	dep	Xman	694:697	arg1	MAN3					727:730	MAN3	727:730	MAN3	727:730	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	5	60	dep	Xman	694:697	arg1	MAN9					742:745	MAN9	742:745	MAN9	742:745	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	5	60	dep	Xman	694:697	arg1	MAN5					733:736	MAN5	733:736	MAN5	733:736	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	2	61	theme	reversible	254:263	arg1	manner					265:270	a specific and reversible manner	239:270	a specific and reversible manner	239:270	Lectins are a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner.					
33616012	8	62	theme	pre-contracted	1231:1244	arg1	rings					1253:1257	pre-contracted aortic rings	1231:1257	pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO)	1231:1347	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	9	63	theme	expanded	1478:1485	arg1	CRD					1487:1489	its expanded CRD	1474:1489	its expanded CRD	1474:1489	These results confirm the ability of DlyL to interact with high-mannose N-glycans with its expanded CRD, supporting the hypothesis that DlyL vasorelaxant activity occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma.					
33616012	0	64	from	lasiophylla	95:105	arg1	lectin					75:80	a vasorelaxant lectin	60:80	a vasorelaxant lectin from Dioclea lasiophylla	60:105	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	0	64	from	lasiophylla	95:105	arg1	properties					46:55	the carbohydrate-binding properties	21:55	the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla	21:105	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	5	65	from	effect	802:807	arg1	vasorelaxation					812:825	vasorelaxation	812:825	vasorelaxation using rat aortic rings	812:848	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	8	66	theme	aortic	1246:1251	arg1	rings					1253:1257	pre-contracted aortic rings	1231:1257	pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO)	1231:1347	Furthermore, aortic rings assays demonstrated that the lectin could relax pre-contracted aortic rings with the participation of the carbohydrate recognition domain (CRD) and nitric oxide (NO) when endothelial tissue is preserved.					
33616012	4	67	theme	lectin	582:587	arg1	DlyL					621:624	DlyL	621:624	DlyL	621:624	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	4	67	theme	lectin	582:587	arg1	study					544:548	a structural and biological study	516:548	a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds	516:618	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	0	68	theme	vasorelaxant	62:73	arg1	lectin					75:80	a vasorelaxant lectin	60:80	a vasorelaxant lectin from Dioclea lasiophylla	60:105	In depth analysis on the carbohydrate-binding properties of a vasorelaxant lectin from Dioclea lasiophylla Mart Ex.					
33616012	2	69	theme	capable	178:184	arg1	Lectins					129:135	Lectins	129:135	Lectins	129:135	Lectins are a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner.					
33616012	2	69	theme	capable	178:184	arg1	class					143:147	a class	141:147	a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner	141:270	Lectins are a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner.					
33616012	2	70	theme	specific	241:248	arg1	manner					265:270	a specific and reversible manner	239:270	a specific and reversible manner	239:270	Lectins are a class of proteins or glycoproteins capable of recognizing and interacting with carbohydrates in a specific and reversible manner.					
33616012	4	71	theme	lasiophylla	602:612	arg1	seeds					614:618	Dioclea lasiophylla seeds	594:618	Dioclea lasiophylla seeds	594:618	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
33616012	6	72	theme	initial	919:925	arg1	coordinates					927:937	the initial coordinates	915:937	the initial coordinates of the DlyL complexes with the carbohydrates	915:982	In silico analysis of molecular docking was performed to obtain the initial coordinates of the DlyL complexes with the carbohydrates to apply as inputs in MD simulations.					
33616012	5	73	theme	molecular	751:759	arg1	dynamics					761:768	molecular dynamics	751:768	molecular dynamics (MD)	751:773	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	5	73	theme	molecular	751:759	arg1	MD					771:772	MD	771:772	MD	771:772	The study aimed to evaluate in detail the interaction of DlyL with Xman and high-mannose N-glycans (MAN3, MAN5 and MAN9) by molecular dynamics (MD) and the resultant in vitro effect on vasorelaxation using rat aortic rings.					
33616012	9	74	theme	vasorelaxant	1528:1539	arg1	activity					1541:1548	DlyL vasorelaxant activity	1523:1548	DlyL vasorelaxant activity	1523:1548	These results confirm the ability of DlyL to interact with high-mannose N-glycans with its expanded CRD, supporting the hypothesis that DlyL vasorelaxant activity occurs primarily through its interaction with cell surface glycosylated receptors.Communicated by Ramaswamy H. Sarma.					
33616012	4	75	theme	Dioclea	594:600	arg1	seeds					614:618	Dioclea lasiophylla seeds	594:618	Dioclea lasiophylla seeds	594:618	Here, we report a structural and biological study of the mannose/glucose-specific lectin from Dioclea lasiophylla seeds, DlyL.					
34931806	0	0	theme	Modifications	80:92	arg1	Reporter					59:66	a Metabolic Chemical Reporter	38:66	a Metabolic Chemical Reporter of O-GlcNAc Modifications	38:92	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	0	0	theme	Modifications	80:92	arg1	4-Deoxy-4-fluoro-GalNAz					0:22	4-Deoxy-4-fluoro-GalNAz	0:22	4-Deoxy-4-fluoro-GalNAz (4FGalNAz)	0:33	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	12	1	theme	important	1908:1916	arg1	implications					1918:1929	interesting and important implications	1892:1929	interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides	1892:2027	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	1	2	theme	Bio-orthogonal	167:180	arg1	chemistries					182:192	Bio-orthogonal chemistries	167:192	Bio-orthogonal chemistries	167:192	Bio-orthogonal chemistries have revolutionized many fields.					
34931806	0	3	theme	O-GlcNAc	71:78	arg1	Modifications					80:92	O-GlcNAc Modifications	71:92	O-GlcNAc Modifications	71:92	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	12	4	theme	large	1851:1855	arg1	flexibility					1867:1877	the large substrate flexibility	1847:1877	the large substrate flexibility of OGT	1847:1884	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	3	5	theme	enrichment	559:568	arg1	tags					570:573	enrichment tags	559:573	enrichment tags	559:573	MCRs are metabolically incorporated into glycoproteins by living systems, and bio-orthogonal reactions can be subsequently employed to install visualization and enrichment tags.					
34931806	10	6	theme	glycosylation	1543:1555	arg1	pathways					1557:1564	endogenous glycosylation pathways	1532:1564	endogenous glycosylation pathways	1532:1564	After synthesis, we found that 4FGalNAz labels a variety of proteins in mammalian cells and does not perturb endogenous glycosylation pathways unlike 4FGalNAc.					
34931806	3	7	gly	glycoproteins	439:451	arg1	glycoproteins					439:451	glycoproteins	439:451	glycoproteins	439:451	MCRs are metabolically incorporated into glycoproteins by living systems, and bio-orthogonal reactions can be subsequently employed to install visualization and enrichment tags.					
34931806	2	8	theme	monosaccharides	310:324	arg1	reporters					259:267	metabolic chemical reporters	240:267	metabolic chemical reporters (MCRs) of glycosylation	240:291	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	8	theme	monosaccharides	310:324	arg1	alkynes					389:395	alkynes	389:395	alkynes	389:395	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	8	theme	monosaccharides	310:324	arg1	analogues					297:305	analogues	297:305	analogues	297:305	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	8	theme	monosaccharides	310:324	arg1	azides					379:384	azides	379:384	azides	379:384	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	4	9	theme	glycosylation	636:648	arg1	class					627:631	one class	623:631	one class of glycosylation (e.g., N-linked vs O-linked)	623:677	Unfortunately, most MCRs are not selective for one class of glycosylation (e.g., N-linked vs O-linked), complicating the types of information that can be gleaned.					
34931806	10	10	theme	endogenous	1532:1541	arg1	pathways					1557:1564	endogenous glycosylation pathways	1532:1564	endogenous glycosylation pathways	1532:1564	After synthesis, we found that 4FGalNAz labels a variety of proteins in mammalian cells and does not perturb endogenous glycosylation pathways unlike 4FGalNAc.					
34931806	6	11	link	O-linked	1034:1041	arg1	glycosylation					1043:1055	mucin O-linked glycosylation	1028:1055	mucin O-linked glycosylation	1028:1055	Here, we attempt to do the same for the core GalNAc residue of mucin O-linked glycosylation.					
34931806	0	12	theme	Notable	112:118	arg1	Flexibility					130:140	the Notable Substrate Flexibility	108:140	the Notable Substrate Flexibility of O-GlcNAc Transferase	108:164	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	9	13	theme	GalNAz	1304:1309	arg1	4-hydroxyl					1290:1299	4-hydroxyl	1290:1299	4-hydroxyl	1290:1299	We reasoned that replacing the 4-hydroxyl of GalNAz with a fluorine would lock the stereochemistry of this position in place, causing the MCR to be more selective.					
34931806	7	14	theme	mucin	1090:1094	arg1	GalNAz					1120:1125	GalNAz	1120:1125	GalNAz	1120:1125	The most widely applied MCR for mucin O-linked glycosylation, GalNAz, can be enzymatically epimerized at the 4-hydroxyl to give GlcNAz.					
34931806	7	14	theme	mucin	1090:1094	arg1	glycosylation					1105:1117	mucin O-linked glycosylation	1090:1117	mucin O-linked glycosylation	1090:1117	The most widely applied MCR for mucin O-linked glycosylation, GalNAz, can be enzymatically epimerized at the 4-hydroxyl to give GlcNAz.					
34931806	8	15	theme	O-GlcNAc	1240:1247	arg1	mixture					1212:1218	a mixture	1210:1218	a mixture of cell-surface and O-GlcNAc labeling	1210:1256	This results in a mixture of cell-surface and O-GlcNAc labeling.					
34931806	8	16	theme	cell-surface	1223:1234	arg1	mixture					1212:1218	a mixture	1210:1218	a mixture of cell-surface and O-GlcNAc labeling	1210:1256	This results in a mixture of cell-surface and O-GlcNAc labeling.					
34931806	12	17	theme	native	2006:2011	arg1	monosaccharides					2013:2027	abiotic and native monosaccharides	1994:2027	abiotic and native monosaccharides	1994:2027	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	7	18	theme	O-linked	1096:1103	arg1	GalNAz					1120:1125	GalNAz	1120:1125	GalNAz	1120:1125	The most widely applied MCR for mucin O-linked glycosylation, GalNAz, can be enzymatically epimerized at the 4-hydroxyl to give GlcNAz.					
34931806	7	18	theme	O-linked	1096:1103	arg1	glycosylation					1105:1117	mucin O-linked glycosylation	1090:1117	mucin O-linked glycosylation	1090:1117	The most widely applied MCR for mucin O-linked glycosylation, GalNAz, can be enzymatically epimerized at the 4-hydroxyl to give GlcNAz.					
34931806	7	19	theme	applied	1074:1080	arg1	MCR					1082:1084	The most widely applied MCR	1058:1084	The most widely applied MCR for mucin O-linked glycosylation, GalNAz,	1058:1126	The most widely applied MCR for mucin O-linked glycosylation, GalNAz, can be enzymatically epimerized at the 4-hydroxyl to give GlcNAz.					
34931806	1	20	theme	revolutionized	199:212	arg1	fields					219:224	revolutionized many fields	199:224	revolutionized many fields	199:224	Bio-orthogonal chemistries have revolutionized many fields.					
34931806	12	21	theme	potential	1975:1983	arg1	range					1985:1989	a potential range	1973:1989	a potential range of abiotic and native monosaccharides	1973:2027	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	10	22	theme	mammalian	1495:1503	arg1	cells					1505:1509	mammalian cells	1495:1509	mammalian cells	1495:1509	After synthesis, we found that 4FGalNAz labels a variety of proteins in mammalian cells and does not perturb endogenous glycosylation pathways unlike 4FGalNAc.					
34931806	12	23	theme	interesting	1892:1902	arg1	implications					1918:1929	interesting and important implications	1892:1929	interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides	1892:2027	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	2	24	theme	bio-orthogonal	341:354	arg1	functionality					356:368	a bio-orthogonal functionality	339:368	a bio-orthogonal functionality	339:368	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	10	25	theme	proteins	1483:1490	arg1	proteins					1483:1490	proteins	1483:1490	proteins	1483:1490	After synthesis, we found that 4FGalNAz labels a variety of proteins in mammalian cells and does not perturb endogenous glycosylation pathways unlike 4FGalNAc.					
34931806	10	25	theme	proteins	1483:1490	arg1	variety					1472:1478	a variety	1470:1478	a variety of proteins	1470:1490	After synthesis, we found that 4FGalNAz labels a variety of proteins in mammalian cells and does not perturb endogenous glycosylation pathways unlike 4FGalNAc.					
34931806	1	26	theme	many	214:217	arg1	fields					219:224	revolutionized many fields	199:224	revolutionized many fields	199:224	Bio-orthogonal chemistries have revolutionized many fields.					
34931806	12	27	theme	intracellular	1935:1947	arg1	modification					1957:1968	intracellular protein modification	1935:1968	intracellular protein modification by a potential range of abiotic and native monosaccharides	1935:2027	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	12	28	theme	OGT	1882:1884	arg1	flexibility					1867:1877	the large substrate flexibility	1847:1877	the large substrate flexibility of OGT	1847:1884	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	7	29	link	O-linked	1096:1103	arg1	GalNAz					1120:1125	GalNAz	1120:1125	GalNAz	1120:1125	The most widely applied MCR for mucin O-linked glycosylation, GalNAz, can be enzymatically epimerized at the 4-hydroxyl to give GlcNAz.					
34931806	7	29	link	O-linked	1096:1103	arg1	glycosylation					1105:1117	mucin O-linked glycosylation	1090:1117	mucin O-linked glycosylation	1090:1117	The most widely applied MCR for mucin O-linked glycosylation, GalNAz, can be enzymatically epimerized at the 4-hydroxyl to give GlcNAz.					
34931806	12	30	theme	substrate	1857:1865	arg1	flexibility					1867:1877	the large substrate flexibility	1847:1877	the large substrate flexibility of OGT	1847:1884	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	8	31	dep	cell-surface	1223:1234	arg1	labeling					1249:1256	labeling	1249:1256	labeling	1249:1256	This results in a mixture of cell-surface and O-GlcNAc labeling.					
34931806	9	32	theme	position	1366:1373	arg1	stereochemistry					1342:1356	the stereochemistry	1338:1356	the stereochemistry of this position in place	1338:1382	We reasoned that replacing the 4-hydroxyl of GalNAz with a fluorine would lock the stereochemistry of this position in place, causing the MCR to be more selective.					
34931806	0	33	theme	Substrate	120:128	arg1	Flexibility					130:140	the Notable Substrate Flexibility	108:140	the Notable Substrate Flexibility of O-GlcNAc Transferase	108:164	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	4	34	dep	glycosylation	636:648	arg1	O-linked					669:676	O-linked	669:676	O-linked	669:676	Unfortunately, most MCRs are not selective for one class of glycosylation (e.g., N-linked vs O-linked), complicating the types of information that can be gleaned.					
34931806	4	34	dep	glycosylation	636:648	arg1	N-linked					657:664	N-linked	657:664	N-linked	657:664	Unfortunately, most MCRs are not selective for one class of glycosylation (e.g., N-linked vs O-linked), complicating the types of information that can be gleaned.					
34931806	12	35	theme	abiotic	1994:2000	arg1	monosaccharides					2013:2027	abiotic and native monosaccharides	1994:2027	abiotic and native monosaccharides	1994:2027	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	6	36	theme	glycosylation	1043:1055	arg1	glycosylation					1043:1055	mucin O-linked glycosylation	1028:1055	mucin O-linked glycosylation	1028:1055	Here, we attempt to do the same for the core GalNAc residue of mucin O-linked glycosylation.					
34931806	6	36	theme	glycosylation	1043:1055	arg1	residue					1017:1023	the core GalNAc residue	1001:1023	the core GalNAc residue of mucin O-linked glycosylation	1001:1055	Here, we attempt to do the same for the core GalNAc residue of mucin O-linked glycosylation.					
34931806	12	37	theme	protein	1949:1955	arg1	modification					1957:1968	intracellular protein modification	1935:1968	intracellular protein modification by a potential range of abiotic and native monosaccharides	1935:2027	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	11	38	gly	glycoproteins	1713:1725	arg1	glycoproteins					1713:1725	cell-surface glycoproteins	1700:1725	cell-surface glycoproteins	1700:1725	However, through subsequent proteomic and biochemical characterization, we found that 4FGalNAz does not widely label cell-surface glycoproteins but instead is primarily a substrate for OGT.					
34931806	0	39	theme	O-GlcNAc	145:152	arg1	Transferase					154:164	O-GlcNAc Transferase	145:164	O-GlcNAc Transferase	145:164	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	6	40	theme	O-linked	1034:1041	arg1	glycosylation					1043:1055	mucin O-linked glycosylation	1028:1055	mucin O-linked glycosylation	1028:1055	Here, we attempt to do the same for the core GalNAc residue of mucin O-linked glycosylation.					
34931806	5	41	theme	MCR	876:878	arg1	structure					859:867	the structure	855:867	the structure of the MCR	855:878	We and others have successfully created MCRs that are selective for intracellular O-GlcNAc modification by altering the structure of the MCR and thus biasing it to certain metabolic pathways and/or O-GlcNAc transferase (OGT).					
34931806	3	42	theme	living	456:461	arg1	systems					463:469	living systems	456:469	living systems	456:469	MCRs are metabolically incorporated into glycoproteins by living systems, and bio-orthogonal reactions can be subsequently employed to install visualization and enrichment tags.					
34931806	5	43	theme	intracellular	807:819	arg1	modification					830:841	intracellular O-GlcNAc modification	807:841	intracellular O-GlcNAc modification	807:841	We and others have successfully created MCRs that are selective for intracellular O-GlcNAc modification by altering the structure of the MCR and thus biasing it to certain metabolic pathways and/or O-GlcNAc transferase (OGT).					
34931806	6	44	theme	mucin	1028:1032	arg1	glycosylation					1043:1055	mucin O-linked glycosylation	1028:1055	mucin O-linked glycosylation	1028:1055	Here, we attempt to do the same for the core GalNAc residue of mucin O-linked glycosylation.					
34931806	0	45	theme	Chemical	50:57	arg1	Reporter					59:66	a Metabolic Chemical Reporter	38:66	a Metabolic Chemical Reporter of O-GlcNAc Modifications	38:92	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	0	45	theme	Chemical	50:57	arg1	4-Deoxy-4-fluoro-GalNAz					0:22	4-Deoxy-4-fluoro-GalNAz	0:22	4-Deoxy-4-fluoro-GalNAz (4FGalNAz)	0:33	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	2	46	contain	contain	331:337	arg2	functionality					356:368	a bio-orthogonal functionality	339:368	a bio-orthogonal functionality	339:368	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	46	contain	contain	331:337	arg1	monosaccharides					310:324	monosaccharides	310:324	monosaccharides that contain a bio-orthogonal functionality	310:368	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	1	47	contain	have	194:197	arg2	fields					219:224	revolutionized many fields	199:224	revolutionized many fields	199:224	Bio-orthogonal chemistries have revolutionized many fields.					
34931806	1	47	contain	have	194:197	arg1	chemistries					182:192	Bio-orthogonal chemistries	167:192	Bio-orthogonal chemistries	167:192	Bio-orthogonal chemistries have revolutionized many fields.					
34931806	2	48	theme	chemical	250:257	arg1	reporters					259:267	metabolic chemical reporters	240:267	metabolic chemical reporters (MCRs) of glycosylation	240:291	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	48	theme	chemical	250:257	arg1	azides					379:384	azides	379:384	azides	379:384	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	48	theme	chemical	250:257	arg1	alkynes					389:395	alkynes	389:395	alkynes	389:395	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	48	theme	chemical	250:257	arg1	analogues					297:305	analogues	297:305	analogues	297:305	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	48	theme	chemical	250:257	arg1	MCRs					270:273	MCRs	270:273	MCRs	270:273	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	5	49	theme	O-GlcNAc	937:944	arg1	transferase					946:956	O-GlcNAc transferase	937:956	O-GlcNAc transferase (OGT)	937:962	We and others have successfully created MCRs that are selective for intracellular O-GlcNAc modification by altering the structure of the MCR and thus biasing it to certain metabolic pathways and/or O-GlcNAc transferase (OGT).					
34931806	5	49	theme	O-GlcNAc	937:944	arg1	OGT					959:961	OGT	959:961	OGT	959:961	We and others have successfully created MCRs that are selective for intracellular O-GlcNAc modification by altering the structure of the MCR and thus biasing it to certain metabolic pathways and/or O-GlcNAc transferase (OGT).					
34931806	0	50	theme	Metabolic	40:48	arg1	Reporter					59:66	a Metabolic Chemical Reporter	38:66	a Metabolic Chemical Reporter of O-GlcNAc Modifications	38:92	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	0	50	theme	Metabolic	40:48	arg1	4-Deoxy-4-fluoro-GalNAz					0:22	4-Deoxy-4-fluoro-GalNAz	0:22	4-Deoxy-4-fluoro-GalNAz (4FGalNAz)	0:33	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	0	51	theme	Transferase	154:164	arg1	Flexibility					130:140	the Notable Substrate Flexibility	108:140	the Notable Substrate Flexibility of O-GlcNAc Transferase	108:164	4-Deoxy-4-fluoro-GalNAz (4FGalNAz) Is a Metabolic Chemical Reporter of O-GlcNAc Modifications, Highlighting the Notable Substrate Flexibility of O-GlcNAc Transferase.					
34931806	4	52	dep	N-linked	657:664	arg1	e.g.					651:654	e.g.	651:654	e.g.	651:654	Unfortunately, most MCRs are not selective for one class of glycosylation (e.g., N-linked vs O-linked), complicating the types of information that can be gleaned.					
34931806	2	53	theme	metabolic	240:248	arg1	reporters					259:267	metabolic chemical reporters	240:267	metabolic chemical reporters (MCRs) of glycosylation	240:291	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	53	theme	metabolic	240:248	arg1	azides					379:384	azides	379:384	azides	379:384	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	53	theme	metabolic	240:248	arg1	alkynes					389:395	alkynes	389:395	alkynes	389:395	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	53	theme	metabolic	240:248	arg1	analogues					297:305	analogues	297:305	analogues	297:305	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	53	theme	metabolic	240:248	arg1	MCRs					270:273	MCRs	270:273	MCRs	270:273	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	9	54	from	stereochemistry	1342:1356	arg1	place					1378:1382	place	1378:1382	place	1378:1382	We reasoned that replacing the 4-hydroxyl of GalNAz with a fluorine would lock the stereochemistry of this position in place, causing the MCR to be more selective.					
34931806	4	55	theme	most	591:594	arg1	MCRs					596:599	most MCRs	591:599	most MCRs	591:599	Unfortunately, most MCRs are not selective for one class of glycosylation (e.g., N-linked vs O-linked), complicating the types of information that can be gleaned.					
34931806	2	56	theme	glycosylation	279:291	arg1	reporters					259:267	metabolic chemical reporters	240:267	metabolic chemical reporters (MCRs) of glycosylation	240:291	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	56	theme	glycosylation	279:291	arg1	azides					379:384	azides	379:384	azides	379:384	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	56	theme	glycosylation	279:291	arg1	alkynes					389:395	alkynes	389:395	alkynes	389:395	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	56	theme	glycosylation	279:291	arg1	analogues					297:305	analogues	297:305	analogues	297:305	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	2	56	theme	glycosylation	279:291	arg1	MCRs					270:273	MCRs	270:273	MCRs	270:273	For example, metabolic chemical reporters (MCRs) of glycosylation are analogues of monosaccharides that contain a bio-orthogonal functionality, such as azides or alkynes.					
34931806	12	57	theme	monosaccharides	2013:2027	arg1	range					1985:1989	a potential range	1973:1989	a potential range of abiotic and native monosaccharides	1973:2027	Although these results are somewhat unexpected, they once again highlight the large substrate flexibility of OGT, with interesting and important implications for intracellular protein modification by a potential range of abiotic and native monosaccharides.					
34931806	5	58	theme	metabolic	911:919	arg1	pathways					921:928	certain metabolic pathways	903:928	certain metabolic pathways	903:928	We and others have successfully created MCRs that are selective for intracellular O-GlcNAc modification by altering the structure of the MCR and thus biasing it to certain metabolic pathways and/or O-GlcNAc transferase (OGT).					
34931806	11	59	theme	cell-surface	1700:1711	arg1	glycoproteins					1713:1725	cell-surface glycoproteins	1700:1725	cell-surface glycoproteins	1700:1725	However, through subsequent proteomic and biochemical characterization, we found that 4FGalNAz does not widely label cell-surface glycoproteins but instead is primarily a substrate for OGT.					
34931806	11	60	theme	subsequent	1600:1609	arg1	proteomic					1611:1619	subsequent proteomic	1600:1619	subsequent proteomic	1600:1619	However, through subsequent proteomic and biochemical characterization, we found that 4FGalNAz does not widely label cell-surface glycoproteins but instead is primarily a substrate for OGT.					
34931806	5	61	theme	certain	903:909	arg1	pathways					921:928	certain metabolic pathways	903:928	certain metabolic pathways	903:928	We and others have successfully created MCRs that are selective for intracellular O-GlcNAc modification by altering the structure of the MCR and thus biasing it to certain metabolic pathways and/or O-GlcNAc transferase (OGT).					
34931806	3	62	theme	bio-orthogonal	476:489	arg1	reactions					491:499	bio-orthogonal reactions	476:499	bio-orthogonal reactions	476:499	MCRs are metabolically incorporated into glycoproteins by living systems, and bio-orthogonal reactions can be subsequently employed to install visualization and enrichment tags.					
34931806	6	63	theme	GalNAc	1010:1015	arg1	glycosylation					1043:1055	mucin O-linked glycosylation	1028:1055	mucin O-linked glycosylation	1028:1055	Here, we attempt to do the same for the core GalNAc residue of mucin O-linked glycosylation.					
34931806	6	63	theme	GalNAc	1010:1015	arg1	residue					1017:1023	the core GalNAc residue	1001:1023	the core GalNAc residue of mucin O-linked glycosylation	1001:1055	Here, we attempt to do the same for the core GalNAc residue of mucin O-linked glycosylation.					
34931806	5	64	theme	O-GlcNAc	821:828	arg1	modification					830:841	intracellular O-GlcNAc modification	807:841	intracellular O-GlcNAc modification	807:841	We and others have successfully created MCRs that are selective for intracellular O-GlcNAc modification by altering the structure of the MCR and thus biasing it to certain metabolic pathways and/or O-GlcNAc transferase (OGT).					
34931806	11	65	theme	biochemical	1625:1635	arg1	characterization					1637:1652	biochemical characterization	1625:1652	biochemical characterization	1625:1652	However, through subsequent proteomic and biochemical characterization, we found that 4FGalNAz does not widely label cell-surface glycoproteins but instead is primarily a substrate for OGT.					
34931806	4	66	theme	information	706:716	arg1	types					697:701	the types	693:701	the types of information that can be gleaned	693:736	Unfortunately, most MCRs are not selective for one class of glycosylation (e.g., N-linked vs O-linked), complicating the types of information that can be gleaned.					
34931806	6	67	theme	core	1005:1008	arg1	glycosylation					1043:1055	mucin O-linked glycosylation	1028:1055	mucin O-linked glycosylation	1028:1055	Here, we attempt to do the same for the core GalNAc residue of mucin O-linked glycosylation.					
34931806	6	67	theme	core	1005:1008	arg1	residue					1017:1023	the core GalNAc residue	1001:1023	the core GalNAc residue of mucin O-linked glycosylation	1001:1055	Here, we attempt to do the same for the core GalNAc residue of mucin O-linked glycosylation.					
34758294	3	0	theme	unbiased	332:339	arg1	approaches					341:350	unbiased approaches	332:350	unbiased approaches	332:350	Using unbiased approaches, we observed an unexpected convergence: RTN4 receptors are high-affinity ligands for BAI adhesion-GPCRs.					
34758294	4	1	theme	BAIs	511:514	arg1	domain					501:506	A single thrombospondin type 1-repeat (TSR) domain	457:506	A single thrombospondin type 1-repeat (TSR) domain of BAIs	457:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	1	theme	BAIs	511:514	arg1	BAIs					511:514	BAIs	511:514	BAIs	511:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	5	2	theme	complex	675:681	arg1	structure					639:647	the 1.65 Å crystal structure	620:647	the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex	620:681	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	1	3	theme	physiological	154:166	arg1	interactors					168:178	their physiological interactors	148:178	their physiological interactors	148:178	RTN4-binding proteins were widely studied as "NoGo" receptors, but their physiological interactors and roles remain elusive.					
34758294	7	4	theme	RTN4/NoGo	1110:1118	arg1	receptors					1120:1128	RTN4/NoGo receptors	1110:1128	RTN4/NoGo receptors	1110:1128	Thus, BAI binding to RTN4/NoGo receptors represents a receptor-ligand axis that, enabled by rare post-translational modifications, controls development of synaptic circuits.					
34758294	4	5	theme	nanomolar	597:605	arg1	affinity					607:614	nanomolar affinity	597:614	nanomolar affinity	597:614	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	5	6	theme	unusual	817:823	arg1	glycoconjugates					825:839	unusual glycoconjugates	817:839	unusual glycoconjugates that enables high-affinity interactions	817:879	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	6	7	theme	human	885:889	arg1	neurons					891:897	human neurons	885:897	human neurons	885:897	In human neurons, RTN4 receptors regulate dendritic arborization, axonal elongation, and synapse formation by differential binding to glial versus neuronal BAIs, thereby controlling neural network activity.					
34758294	5	8	gly	O-fucosylation	718:731	arg1	TSR-domains					757:767	the BAI TSR-domains	749:767	the BAI TSR-domains	749:767	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	5	8	gly	O-fucosylation	718:731	arg1	tryptophan					703:712	tryptophan	703:712	tryptophan	703:712	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	5	8	gly	O-fucosylation	718:731	arg1	threonine					736:744	threonine	736:744	threonine	736:744	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	6	9	theme	network	1071:1077	arg1	activity					1079:1086	neural network activity	1064:1086	neural network activity	1064:1086	In human neurons, RTN4 receptors regulate dendritic arborization, axonal elongation, and synapse formation by differential binding to glial versus neuronal BAIs, thereby controlling neural network activity.					
34758294	3	10	theme	unexpected	368:377	arg1	convergence					379:389	an unexpected convergence	365:389	an unexpected convergence: RTN4 receptors are high-affinity ligands for BAI adhesion-GPCRs	365:454	Using unbiased approaches, we observed an unexpected convergence: RTN4 receptors are high-affinity ligands for BAI adhesion-GPCRs.					
34758294	6	11	theme	synapse	971:977	arg1	formation					979:987	synapse formation	971:987	synapse formation	971:987	In human neurons, RTN4 receptors regulate dendritic arborization, axonal elongation, and synapse formation by differential binding to glial versus neuronal BAIs, thereby controlling neural network activity.					
34758294	7	12	theme	rare	1181:1184	arg1	modifications					1205:1217	rare post-translational modifications	1181:1217	rare post-translational modifications	1181:1217	Thus, BAI binding to RTN4/NoGo receptors represents a receptor-ligand axis that, enabled by rare post-translational modifications, controls development of synaptic circuits.					
34758294	4	13	with	isoforms	583:590	arg1	affinity					607:614	nanomolar affinity	597:614	nanomolar affinity	597:614	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	6	14	theme	neural	1064:1069	arg1	activity					1079:1086	neural network activity	1064:1086	neural network activity	1064:1086	In human neurons, RTN4 receptors regulate dendritic arborization, axonal elongation, and synapse formation by differential binding to glial versus neuronal BAIs, thereby controlling neural network activity.					
34758294	7	15	theme	post-translational	1186:1203	arg1	modifications					1205:1217	rare post-translational modifications	1181:1217	rare post-translational modifications	1181:1217	Thus, BAI binding to RTN4/NoGo receptors represents a receptor-ligand axis that, enabled by rare post-translational modifications, controls development of synaptic circuits.					
34758294	5	16	theme	1.65 Å	624:629	arg1	structure					639:647	the 1.65 Å crystal structure	620:647	the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex	620:681	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	1	17	theme	RTN4-binding	81:92	arg1	proteins					94:101	RTN4-binding proteins	81:101	RTN4-binding proteins	81:101	RTN4-binding proteins were widely studied as "NoGo" receptors, but their physiological interactors and roles remain elusive.					
34758294	1	17	theme	RTN4-binding	81:92	arg1	receptors					133:141	"NoGo" receptors	126:141	"NoGo" receptors	126:141	RTN4-binding proteins were widely studied as "NoGo" receptors, but their physiological interactors and roles remain elusive.					
34758294	5	18	theme	high-affinity	854:866	arg1	interactions					868:879	high-affinity interactions	854:879	high-affinity interactions	854:879	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	4	19	theme	TSR	496:498	arg1	domain					501:506	A single thrombospondin type 1-repeat (TSR) domain	457:506	A single thrombospondin type 1-repeat (TSR) domain of BAIs	457:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	19	theme	TSR	496:498	arg1	BAIs					511:514	BAIs	511:514	BAIs	511:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	5	20	theme	crystal	631:637	arg1	structure					639:647	the 1.65 Å crystal structure	620:647	the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex	620:681	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	5	21	theme	RTN4-receptor/BAI	779:795	arg1	interface					797:805	a RTN4-receptor/BAI interface	777:805	a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions	777:879	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	6	22	theme	axonal	948:953	arg1	elongation					955:964	axonal elongation	948:964	axonal elongation	948:964	In human neurons, RTN4 receptors regulate dendritic arborization, axonal elongation, and synapse formation by differential binding to glial versus neuronal BAIs, thereby controlling neural network activity.					
34758294	0	23	theme	RTN4/NoGo-receptor	0:17	arg1	binding					19:25	RTN4/NoGo-receptor binding	0:25	RTN4/NoGo-receptor binding to BAI adhesion-GPCRs	0:47	RTN4/NoGo-receptor binding to BAI adhesion-GPCRs regulates neuronal development.					
34758294	7	24	theme	synaptic	1244:1251	arg1	circuits					1253:1260	synaptic circuits	1244:1260	synaptic circuits	1244:1260	Thus, BAI binding to RTN4/NoGo receptors represents a receptor-ligand axis that, enabled by rare post-translational modifications, controls development of synaptic circuits.					
34758294	5	25	theme	BAI1/RTN4-receptor	656:673	arg1	complex					675:681	the BAI1/RTN4-receptor complex	652:681	the BAI1/RTN4-receptor complex	652:681	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	5	26	gly	C-mannosylation	684:698	arg1	TSR-domains					757:767	the BAI TSR-domains	749:767	the BAI TSR-domains	749:767	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	5	26	gly	C-mannosylation	684:698	arg1	tryptophan					703:712	tryptophan	703:712	tryptophan	703:712	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	5	26	gly	C-mannosylation	684:698	arg1	threonine					736:744	threonine	736:744	threonine	736:744	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	0	27	theme	BAI	30:32	arg1	adhesion-GPCRs					34:47	BAI adhesion-GPCRs	30:47	BAI adhesion-GPCRs	30:47	RTN4/NoGo-receptor binding to BAI adhesion-GPCRs regulates neuronal development.					
34758294	5	28	theme	threonine	736:744	arg1	O-fucosylation					718:731	O-fucosylation	718:731	O-fucosylation of threonine	718:744	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	5	28	theme	threonine	736:744	arg1	C-mannosylation					684:698	C-mannosylation	684:698	C-mannosylation of tryptophan	684:712	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	3	29	theme	RTN4	392:395	arg1	receptors					397:405	RTN4 receptors	392:405	RTN4 receptors	392:405	Using unbiased approaches, we observed an unexpected convergence: RTN4 receptors are high-affinity ligands for BAI adhesion-GPCRs.					
34758294	3	29	theme	RTN4	392:395	arg1	ligands					425:431	high-affinity ligands	411:431	high-affinity ligands for BAI adhesion-GPCRs	411:454	Using unbiased approaches, we observed an unexpected convergence: RTN4 receptors are high-affinity ligands for BAI adhesion-GPCRs.					
34758294	4	30	theme	isoforms	583:590	arg1	domain					549:554	the leucine-rich repeat domain	525:554	the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity	525:614	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	30	theme	isoforms	583:590	arg1	isoforms					583:590	all three RTN4-receptor isoforms	559:590	all three RTN4-receptor isoforms with nanomolar affinity	559:614	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	31	theme	type	481:484	arg1	domain					501:506	A single thrombospondin type 1-repeat (TSR) domain	457:506	A single thrombospondin type 1-repeat (TSR) domain of BAIs	457:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	31	theme	type	481:484	arg1	BAIs					511:514	BAIs	511:514	BAIs	511:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	32	theme	RTN4-receptor	569:581	arg1	isoforms					583:590	all three RTN4-receptor isoforms	559:590	all three RTN4-receptor isoforms with nanomolar affinity	559:614	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	33	theme	thrombospondin	466:479	arg1	domain					501:506	A single thrombospondin type 1-repeat (TSR) domain	457:506	A single thrombospondin type 1-repeat (TSR) domain of BAIs	457:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	33	theme	thrombospondin	466:479	arg1	BAIs					511:514	BAIs	511:514	BAIs	511:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	6	34	theme	neuronal	1029:1036	arg1	BAIs					1038:1041	glial versus neuronal BAIs	1016:1041	glial versus neuronal BAIs	1016:1041	In human neurons, RTN4 receptors regulate dendritic arborization, axonal elongation, and synapse formation by differential binding to glial versus neuronal BAIs, thereby controlling neural network activity.					
34758294	3	35	theme	BAI	437:439	arg1	adhesion-GPCRs					441:454	BAI adhesion-GPCRs	437:454	BAI adhesion-GPCRs	437:454	Using unbiased approaches, we observed an unexpected convergence: RTN4 receptors are high-affinity ligands for BAI adhesion-GPCRs.					
34758294	4	36	theme	repeat	542:547	arg1	domain					549:554	the leucine-rich repeat domain	525:554	the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity	525:614	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	36	theme	repeat	542:547	arg1	isoforms					583:590	all three RTN4-receptor isoforms	559:590	all three RTN4-receptor isoforms with nanomolar affinity	559:614	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	5	37	theme	BAI	753:755	arg1	TSR-domains					757:767	the BAI TSR-domains	749:767	the BAI TSR-domains	749:767	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	6	38	theme	dendritic	924:932	arg1	arborization					934:945	dendritic arborization	924:945	dendritic arborization	924:945	In human neurons, RTN4 receptors regulate dendritic arborization, axonal elongation, and synapse formation by differential binding to glial versus neuronal BAIs, thereby controlling neural network activity.					
34758294	7	39	theme	circuits	1253:1260	arg1	development					1229:1239	development	1229:1239	development of synaptic circuits	1229:1260	Thus, BAI binding to RTN4/NoGo receptors represents a receptor-ligand axis that, enabled by rare post-translational modifications, controls development of synaptic circuits.					
34758294	3	40	theme	high-affinity	411:423	arg1	receptors					397:405	RTN4 receptors	392:405	RTN4 receptors	392:405	Using unbiased approaches, we observed an unexpected convergence: RTN4 receptors are high-affinity ligands for BAI adhesion-GPCRs.					
34758294	3	40	theme	high-affinity	411:423	arg1	ligands					425:431	high-affinity ligands	411:431	high-affinity ligands for BAI adhesion-GPCRs	411:454	Using unbiased approaches, we observed an unexpected convergence: RTN4 receptors are high-affinity ligands for BAI adhesion-GPCRs.					
34758294	4	41	theme	leucine-rich	529:540	arg1	domain					549:554	the leucine-rich repeat domain	525:554	the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity	525:614	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	41	theme	leucine-rich	529:540	arg1	isoforms					583:590	all three RTN4-receptor isoforms	559:590	all three RTN4-receptor isoforms with nanomolar affinity	559:614	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	5	42	theme	tryptophan	703:712	arg1	O-fucosylation					718:731	O-fucosylation	718:731	O-fucosylation of threonine	718:744	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	5	42	theme	tryptophan	703:712	arg1	C-mannosylation					684:698	C-mannosylation	684:698	C-mannosylation of tryptophan	684:712	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	4	43	theme	1-repeat	486:493	arg1	domain					501:506	A single thrombospondin type 1-repeat (TSR) domain	457:506	A single thrombospondin type 1-repeat (TSR) domain of BAIs	457:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	43	theme	1-repeat	486:493	arg1	BAIs					511:514	BAIs	511:514	BAIs	511:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	7	44	theme	BAI	1095:1097	arg1	binding					1099:1105	BAI binding	1095:1105	BAI binding to RTN4/NoGo receptors	1095:1128	Thus, BAI binding to RTN4/NoGo receptors represents a receptor-ligand axis that, enabled by rare post-translational modifications, controls development of synaptic circuits.					
34758294	1	45	theme	NoGo	127:130	arg1	receptors					133:141	"NoGo" receptors	126:141	"NoGo" receptors	126:141	RTN4-binding proteins were widely studied as "NoGo" receptors, but their physiological interactors and roles remain elusive.					
34758294	1	45	theme	NoGo	127:130	arg1	proteins					94:101	RTN4-binding proteins	81:101	RTN4-binding proteins	81:101	RTN4-binding proteins were widely studied as "NoGo" receptors, but their physiological interactors and roles remain elusive.					
34758294	0	46	theme	neuronal	59:66	arg1	development					68:78	neuronal development	59:78	neuronal development	59:78	RTN4/NoGo-receptor binding to BAI adhesion-GPCRs regulates neuronal development.					
34758294	5	47	from	C-mannosylation	684:698	arg1	TSR-domains					757:767	the BAI TSR-domains	749:767	the BAI TSR-domains	749:767	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	7	48	theme	receptor-ligand	1143:1157	arg1	axis					1159:1162	a receptor-ligand axis	1141:1162	a receptor-ligand axis that, enabled by rare post-translational modifications, controls development of synaptic circuits	1141:1260	Thus, BAI binding to RTN4/NoGo receptors represents a receptor-ligand axis that, enabled by rare post-translational modifications, controls development of synaptic circuits.					
34758294	2	49	theme	BAI	217:219	arg1	adhesion-GPCRs					221:234	BAI adhesion-GPCRs	217:234	BAI adhesion-GPCRs	217:234	Similarly, BAI adhesion-GPCRs were associated with numerous activities, but their ligands and functions remain unclear.					
34758294	1	50	theme	"	131:131	arg1	receptors					133:141	"NoGo" receptors	126:141	"NoGo" receptors	126:141	RTN4-binding proteins were widely studied as "NoGo" receptors, but their physiological interactors and roles remain elusive.					
34758294	1	50	theme	"	131:131	arg1	proteins					94:101	RTN4-binding proteins	81:101	RTN4-binding proteins	81:101	RTN4-binding proteins were widely studied as "NoGo" receptors, but their physiological interactors and roles remain elusive.					
34758294	6	51	theme	RTN4	900:903	arg1	receptors					905:913	RTN4 receptors	900:913	RTN4 receptors	900:913	In human neurons, RTN4 receptors regulate dendritic arborization, axonal elongation, and synapse formation by differential binding to glial versus neuronal BAIs, thereby controlling neural network activity.					
34758294	5	52	from	O-fucosylation	718:731	arg1	TSR-domains					757:767	the BAI TSR-domains	749:767	the BAI TSR-domains	749:767	In the 1.65 Å crystal structure of the BAI1/RTN4-receptor complex, C-mannosylation of tryptophan and O-fucosylation of threonine in the BAI TSR-domains creates a RTN4-receptor/BAI interface shaped by unusual glycoconjugates that enables high-affinity interactions.					
34758294	3	53	dep	convergence	379:389	arg1	receptors					397:405	RTN4 receptors	392:405	RTN4 receptors	392:405	Using unbiased approaches, we observed an unexpected convergence: RTN4 receptors are high-affinity ligands for BAI adhesion-GPCRs.					
34758294	3	53	dep	convergence	379:389	arg1	ligands					425:431	high-affinity ligands	411:431	high-affinity ligands for BAI adhesion-GPCRs	411:454	Using unbiased approaches, we observed an unexpected convergence: RTN4 receptors are high-affinity ligands for BAI adhesion-GPCRs.					
34758294	4	54	theme	single	459:464	arg1	domain					501:506	A single thrombospondin type 1-repeat (TSR) domain	457:506	A single thrombospondin type 1-repeat (TSR) domain of BAIs	457:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	4	54	theme	single	459:464	arg1	BAIs					511:514	BAIs	511:514	BAIs	511:514	A single thrombospondin type 1-repeat (TSR) domain of BAIs binds to the leucine-rich repeat domain of all three RTN4-receptor isoforms with nanomolar affinity.					
34758294	6	55	theme	differential	992:1003	arg1	binding					1005:1011	differential binding	992:1011	differential binding to glial versus neuronal BAIs	992:1041	In human neurons, RTN4 receptors regulate dendritic arborization, axonal elongation, and synapse formation by differential binding to glial versus neuronal BAIs, thereby controlling neural network activity.					
34758294	2	56	theme	numerous	257:264	arg1	activities					266:275	numerous activities	257:275	numerous activities	257:275	Similarly, BAI adhesion-GPCRs were associated with numerous activities, but their ligands and functions remain unclear.					
34758294	6	57	theme	glial	1016:1020	arg1	BAIs					1038:1041	glial versus neuronal BAIs	1016:1041	glial versus neuronal BAIs	1016:1041	In human neurons, RTN4 receptors regulate dendritic arborization, axonal elongation, and synapse formation by differential binding to glial versus neuronal BAIs, thereby controlling neural network activity.					
33605229	4	0	theme	wound	916:920	arg1	healing					922:928	a wound healing	914:928	a wound healing material	914:937	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	2	1	theme	Nostoc	398:403	arg1	commune					405:411	Nostoc commune	398:411	Nostoc commune	398:411	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	5	2	theme	±	1093:1093	arg1	%					1099:1099	79.16 ± 3.91%	1087:1099	79.16 ± 3.91%	1087:1099	Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day.					
33605229	5	3	theme	wound	1030:1034	arg1	healing					1036:1042	wound healing	1030:1042	wound healing (P < 0.01)	1030:1053	Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day.					
33605229	5	3	theme	wound	1030:1034	arg1	<					1047:1047	P < 0.01	1045:1052	P < 0.01	1045:1052	Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day.					
33605229	4	4	theme	In	731:732	arg1	experiments					740:750	In vitro experiments	731:750	In vitro experiments	731:750	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	5	5	theme	Animal	940:945	arg1	experiments					947:957	Animal experiments	940:957	Animal experiments	940:957	Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day.					
33605229	6	6	theme	UAP	1124:1126	arg1	microcapsules					1128:1140	Meanwhile UAP microcapsules	1114:1140	Meanwhile UAP microcapsules	1114:1140	Meanwhile UAP microcapsules might promote angiogenesis and granulation growth by enhancing immunity and increasing the expression of VEGF and miR-21.					
33605229	4	7	theme	porous	803:808	arg1	structure					846:854	a porous and loose three-dimensional network structure	801:854	a porous and loose three-dimensional network structure	801:854	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	3	8	from	effect	580:585	arg1	healing					641:647	wound healing	635:647	wound healing in an experimental rat model for 14 d and its physical properties	635:713	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated.					
33605229	1	9	theme	vascular	300:307	arg1	regeneration					309:320	vascular regeneration	300:320	vascular regeneration	300:320	Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing.					
33605229	4	10	theme	microcapsule	775:786	arg1	material					788:795	the UAP microcapsule material	767:795	the UAP microcapsule material	767:795	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	2	11	from	%	496:496	arg1	study					360:364	this study	355:364	this study	355:364	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	4	12	theme	healing	922:928	arg1	material					930:937	a wound healing material	914:937	a wound healing material	914:937	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	4	13	dep	In	731:732	arg1	vitro					734:738	vitro	734:738	vitro	734:738	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	4	14	contain	had	861:863	arg1	material					788:795	the UAP microcapsule material	767:795	the UAP microcapsule material	767:795	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	4	14	contain	had	861:863	arg2	properties					900:909	good biocompatibility and swelling properties	865:909	good biocompatibility and swelling properties	865:909	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	0	15	theme	Commune	130:136	arg1	Vaucher					138:144	Nostoc Commune Vaucher	123:144	Nostoc Commune Vaucher	123:144	Preparation, characterization and wound healing effect of alginate/chitosan microcapsules loaded with polysaccharides from Nostoc Commune Vaucher.					
33605229	4	16	theme	UAP	771:773	arg1	material					788:795	the UAP microcapsule material	767:795	the UAP microcapsule material	767:795	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	4	17	theme	swelling	891:898	arg1	properties					900:909	good biocompatibility and swelling properties	865:909	good biocompatibility and swelling properties	865:909	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	4	18	theme	loose	814:818	arg1	structure					846:854	a porous and loose three-dimensional network structure	801:854	a porous and loose three-dimensional network structure	801:854	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	0	19	theme	Nostoc	123:128	arg1	Commune					130:136	Nostoc Commune	123:136	Nostoc Commune Vaucher	123:144	Preparation, characterization and wound healing effect of alginate/chitosan microcapsules loaded with polysaccharides from Nostoc Commune Vaucher.					
33605229	6	20	theme	Meanwhile	1114:1122	arg1	microcapsules					1128:1140	Meanwhile UAP microcapsules	1114:1140	Meanwhile UAP microcapsules	1114:1140	Meanwhile UAP microcapsules might promote angiogenesis and granulation growth by enhancing immunity and increasing the expression of VEGF and miR-21.					
33605229	2	21	theme	±	490:490	arg1	%					496:496	12.89 ± 0.24%	484:496	12.89 ± 0.24%	484:496	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	3	22	theme	composite	612:620	arg1	materials					622:630	alginate/chitosan-UAP composite materials	590:630	alginate/chitosan-UAP composite materials	590:630	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated.					
33605229	0	23	theme	Preparation	0:10	arg1	effect					48:53	Preparation, characterization and wound healing effect	0:53	Preparation, characterization and wound healing effect of alginate/chitosan	0:74	Preparation, characterization and wound healing effect of alginate/chitosan microcapsules loaded with polysaccharides from Nostoc Commune Vaucher.					
33605229	3	24	theme	materials	622:630	arg1	effect					580:585	The effect	576:585	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties	576:713	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated.					
33605229	1	25	theme	granulation	213:223	arg1	tissue					225:230	granulation tissue	213:230	granulation tissue	213:230	Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing.					
33605229	1	26	theme	wound	326:330	arg1	healing					332:338	wound healing	326:338	wound healing	326:338	Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing.					
33605229	4	27	theme	good	865:868	arg1	properties					900:909	good biocompatibility and swelling properties	865:909	good biocompatibility and swelling properties	865:909	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	0	28	dep	microcapsules	76:88	arg1	loaded					90:95	loaded	90:95	microcapsules loaded with polysaccharides from Nostoc Commune Vaucher	76:144	Preparation, characterization and wound healing effect of alginate/chitosan microcapsules loaded with polysaccharides from Nostoc Commune Vaucher.					
33605229	2	29	theme	process	459:465	arg1	optimization					467:478	the process optimization	455:478	the process optimization	455:478	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	0	30	theme	characterization	13:28	arg1	effect					48:53	Preparation, characterization and wound healing effect	0:53	Preparation, characterization and wound healing effect of alginate/chitosan	0:74	Preparation, characterization and wound healing effect of alginate/chitosan microcapsules loaded with polysaccharides from Nostoc Commune Vaucher.					
33605229	5	31	theme	P	1045:1045	arg1	healing					1036:1042	wound healing	1030:1042	wound healing (P < 0.01)	1030:1053	Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day.					
33605229	5	31	theme	P	1045:1045	arg1	<					1047:1047	P < 0.01	1045:1052	P < 0.01	1045:1052	Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day.					
33605229	3	32	theme	alginate/chitosan-UAP	590:610	arg1	materials					622:630	alginate/chitosan-UAP composite materials	590:630	alginate/chitosan-UAP composite materials	590:630	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated.					
33605229	3	33	from	healing	641:647	arg1	properties					704:713	its physical properties	691:713	its physical properties	691:713	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated.					
33605229	3	33	from	healing	641:647	arg1	model					672:676	an experimental rat model	652:676	an experimental rat model for 14 d	652:685	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated.					
33605229	2	34	theme	Vaucher	413:419	arg1	UAP					438:440	UAP	438:440	UAP	438:440	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	2	34	theme	Vaucher	413:419	arg1	polysaccharides					421:435	Vaucher polysaccharides	413:435	Vaucher polysaccharides (UAP)	413:441	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	0	35	theme	healing	40:46	arg1	effect					48:53	Preparation, characterization and wound healing effect	0:53	Preparation, characterization and wound healing effect of alginate/chitosan	0:74	Preparation, characterization and wound healing effect of alginate/chitosan microcapsules loaded with polysaccharides from Nostoc Commune Vaucher.					
33605229	5	36	theme	UAP	974:976	arg1	microcapsules					978:990	UAP microcapsules	974:990	UAP microcapsules	974:990	Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day.					
33605229	7	37	theme	UAP	1293:1295	arg1	microcapsules					1297:1309	UAP microcapsules	1293:1309	UAP microcapsules	1293:1309	Therefore, the composites of UAP microcapsules have shown encouraging results as a potential dressing for wound healing.					
33605229	5	38	theme	wound	1060:1064	arg1	closure					1066:1072	wound closure	1060:1072	wound closure rate	1060:1077	Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day.					
33605229	7	39	theme	wound	1370:1374	arg1	healing					1376:1382	wound healing	1370:1382	wound healing	1370:1382	Therefore, the composites of UAP microcapsules have shown encouraging results as a potential dressing for wound healing.					
33605229	1	40	theme	auxiliary	235:243	arg1	growth					203:208	the growth	199:208	the growth of granulation tissue	199:230	Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing.					
33605229	1	40	theme	auxiliary	235:243	arg1	materials					245:253	auxiliary materials	235:253	auxiliary materials	235:253	Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing.					
33605229	0	41	theme	wound	34:38	arg1	healing					40:46	wound healing	34:46	wound healing	34:46	Preparation, characterization and wound healing effect of alginate/chitosan microcapsules loaded with polysaccharides from Nostoc Commune Vaucher.					
33605229	5	42	theme	closure	1066:1072	arg1	rate					1074:1077	wound closure rate	1060:1077	wound closure rate	1060:1077	Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day.					
33605229	4	43	contain	had	797:799	arg1	material					788:795	the UAP microcapsule material	767:795	the UAP microcapsule material	767:795	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	4	43	contain	had	797:799	arg2	structure					846:854	a porous and loose three-dimensional network structure	801:854	a porous and loose three-dimensional network structure	801:854	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	4	44	theme	biocompatibility	870:885	arg1	properties					900:909	good biocompatibility and swelling properties	865:909	good biocompatibility and swelling properties	865:909	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	6	45	theme	granulation	1173:1183	arg1	growth					1185:1190	granulation growth	1173:1190	granulation growth	1173:1190	Meanwhile UAP microcapsules might promote angiogenesis and granulation growth by enhancing immunity and increasing the expression of VEGF and miR-21.					
33605229	2	46	theme	0.24	492:495	arg1	%					496:496	12.89 ± 0.24%	484:496	12.89 ± 0.24%	484:496	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	5	47	theme	79.16	1087:1091	arg1	%					1099:1099	79.16 ± 3.91%	1087:1099	79.16 ± 3.91%	1087:1099	Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day.					
33605229	3	48	theme	wound	635:639	arg1	healing					641:647	wound healing	635:647	wound healing in an experimental rat model for 14 d and its physical properties	635:713	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated.					
33605229	7	49	theme	encouraging	1322:1332	arg1	dressing					1357:1364	a potential dressing	1345:1364	a potential dressing for wound healing	1345:1382	Therefore, the composites of UAP microcapsules have shown encouraging results as a potential dressing for wound healing.					
33605229	7	49	theme	encouraging	1322:1332	arg1	results					1334:1340	encouraging results	1322:1340	encouraging results	1322:1340	Therefore, the composites of UAP microcapsules have shown encouraging results as a potential dressing for wound healing.					
33605229	2	50	theme	ultrasound-assisted	367:385	arg1	extract					387:393	ultrasound-assisted extract	367:393	ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization	367:478	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	7	51	theme	microcapsules	1297:1309	arg1	composites					1279:1288	the composites	1275:1288	the composites of UAP microcapsules	1275:1309	Therefore, the composites of UAP microcapsules have shown encouraging results as a potential dressing for wound healing.					
33605229	7	51	theme	microcapsules	1297:1309	arg1	microcapsules					1297:1309	UAP microcapsules	1293:1309	UAP microcapsules	1293:1309	Therefore, the composites of UAP microcapsules have shown encouraging results as a potential dressing for wound healing.					
33605229	2	52	used	used	509:512	arg2	%					496:496	12.89 ± 0.24%	484:496	12.89 ± 0.24%	484:496	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	3	53	theme	rat	668:670	arg1	model					672:676	an experimental rat model	652:676	an experimental rat model for 14 d	652:685	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated.					
33605229	2	54	dep	extract	387:393	arg1	yield					443:447	yield	443:447	yield after the process optimization	443:478	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	6	55	theme	miR-21	1256:1261	arg1	expression					1233:1242	the expression	1229:1242	the expression of VEGF and miR-21	1229:1261	Meanwhile UAP microcapsules might promote angiogenesis and granulation growth by enhancing immunity and increasing the expression of VEGF and miR-21.					
33605229	4	56	theme	three-dimensional	820:836	arg1	structure					846:854	a porous and loose three-dimensional network structure	801:854	a porous and loose three-dimensional network structure	801:854	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	1	57	theme	active	160:165	arg1	materials					175:183	Biologically active coating materials	147:183	Biologically active coating materials	147:183	Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing.					
33605229	4	58	theme	network	838:844	arg1	structure					846:854	a porous and loose three-dimensional network structure	801:854	a porous and loose three-dimensional network structure	801:854	In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material.					
33605229	3	59	theme	physical	695:702	arg1	properties					704:713	its physical properties	691:713	its physical properties	691:713	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated.					
33605229	6	60	theme	VEGF	1247:1250	arg1	expression					1233:1242	the expression	1229:1242	the expression of VEGF and miR-21	1229:1261	Meanwhile UAP microcapsules might promote angiogenesis and granulation growth by enhancing immunity and increasing the expression of VEGF and miR-21.					
33605229	1	61	theme	tissue	225:230	arg1	growth					203:208	the growth	199:208	the growth of granulation tissue	199:230	Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing.					
33605229	1	61	theme	tissue	225:230	arg1	materials					245:253	auxiliary materials	235:253	auxiliary materials	235:253	Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing.					
33605229	1	62	theme	coating	167:173	arg1	materials					175:183	Biologically active coating materials	147:183	Biologically active coating materials	147:183	Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing.					
33605229	7	63	theme	potential	1347:1355	arg1	dressing					1357:1364	a potential dressing	1345:1364	a potential dressing for wound healing	1345:1382	Therefore, the composites of UAP microcapsules have shown encouraging results as a potential dressing for wound healing.					
33605229	7	63	theme	potential	1347:1355	arg1	results					1334:1340	encouraging results	1322:1340	encouraging results	1322:1340	Therefore, the composites of UAP microcapsules have shown encouraging results as a potential dressing for wound healing.					
33605229	1	64	theme	natural	262:268	arg1	polysaccharides					270:284	natural polysaccharides	262:284	natural polysaccharides	262:284	Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing.					
33605229	0	65	theme	alginate/chitosan	58:74	arg1	effect					48:53	Preparation, characterization and wound healing effect	0:53	Preparation, characterization and wound healing effect of alginate/chitosan	0:74	Preparation, characterization and wound healing effect of alginate/chitosan microcapsules loaded with polysaccharides from Nostoc Commune Vaucher.					
33605229	3	66	theme	experimental	655:666	arg1	model					672:676	an experimental rat model	652:676	an experimental rat model for 14 d	652:685	The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated.					
33605229	2	67	theme	commune	405:411	arg1	extract					387:393	ultrasound-assisted extract	367:393	ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization	367:478	Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking.					
33605229	0	68	from	Vaucher	138:144	arg1	polysaccharides					102:116	polysaccharides	102:116	polysaccharides from Nostoc Commune Vaucher	102:144	Preparation, characterization and wound healing effect of alginate/chitosan microcapsules loaded with polysaccharides from Nostoc Commune Vaucher.					
32149355	5	0	theme	exoglycosidase	862:875	arg1	digestion					877:885	exoglycosidase digestion	862:885	exoglycosidase digestion of matriglycan	862:900	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	1	1	theme	α-dystroglycan	181:194	arg1	O-mannosylation					162:176	proper O-mannosylation	155:176	proper O-mannosylation of α-dystroglycan	155:194	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	4	2	theme	terminal	641:648	arg1	matriglycan					673:683	matriglycan	673:683	matriglycan	673:683	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	4	2	theme	terminal	641:648	arg1	residues					661:668	terminal glucuronyl residues	641:668	terminal glucuronyl residues of matriglycan	641:683	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	5	3	dep	functional	907:916	arg1	molecular					925:933	molecular	925:933	molecular	925:933	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	2	4	theme	functional	347:356	arg1	-GlcA-β3-Xyl-α3-					422:437	-GlcA-β3-Xyl-α3-	422:437	-GlcA-β3-Xyl-α3-	422:437	Previously, we and others further elucidated the functional O-mannose glycan structure that is terminated by matriglycan, [(-GlcA-β3-Xyl-α3-)n].					
32149355	2	4	theme	functional	347:356	arg1	structure					375:383	the functional O-mannose glycan structure	343:383	the functional O-mannose glycan structure that is terminated by matriglycan	343:417	Previously, we and others further elucidated the functional O-mannose glycan structure that is terminated by matriglycan, [(-GlcA-β3-Xyl-α3-)n].					
32149355	5	5	attach	isolated	807:814	arg1	kidney					837:842	kidney	837:842	kidney	837:842	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	5	5	attach	isolated	807:814	arg1	heart					827:831	mouse heart	821:831	mouse heart	821:831	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	5	5	attach	isolated	807:814	arg2	α-dystroglycan					792:805	α-dystroglycan	792:805	α-dystroglycan isolated from mouse heart and kidney	792:842	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	4	6	theme	glucuronyl	650:659	arg1	matriglycan					673:683	matriglycan	673:683	matriglycan	673:683	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	4	6	theme	glucuronyl	650:659	arg1	residues					661:668	terminal glucuronyl residues	641:668	terminal glucuronyl residues of matriglycan	641:683	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	0	7	from	3-O-sulfation	65:77	arg1	matriglycan					101:111	matriglycan	101:111	matriglycan	101:111	HNK-1 sulfotransferase modulates α-dystroglycan glycosylation by 3-O-sulfation of glucuronic acid on matriglycan.					
32149355	6	8	theme	dual-glycosidase	1082:1097	arg1	digestion					1099:1107	dual-glycosidase digestion	1082:1107	dual-glycosidase digestion	1082:1107	Removal of the sulfate cap by a sulfatase facilitated dual-glycosidase digestion.					
32149355	7	9	theme	nonreducing	1256:1266	arg1	GlcA					1268:1271	the nonreducing GlcA	1252:1271	the nonreducing GlcA of matriglycan	1252:1286	Our data strongly support a tissue specific mechanism in which HNK-1ST regulates polymer length by competing with LARGE for the 3-position on the nonreducing GlcA of matriglycan.					
32149355	4	10	theme	matriglycan	724:734	arg1	polymerization					736:749	further matriglycan polymerization	716:749	further matriglycan polymerization	716:749	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	5	11	theme	functional	907:916	arg1	resistant					1017:1025	resistant	1017:1025	resistant	1017:1025	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	5	11	theme	functional	907:916	arg1	α-dystroglycan					942:955	the functional, lower molecular weight α-dystroglycan	903:955	the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated,	903:1012	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	1	12	theme	congenital/limb-girdle	211:232	arg1	dystrophies					243:253	congenital/limb-girdle muscular dystrophies	211:253	congenital/limb-girdle muscular dystrophies	211:253	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	0	13	theme	HNK-1	0:4	arg1	sulfotransferase					6:21	HNK-1 sulfotransferase	0:21	HNK-1 sulfotransferase	0:21	HNK-1 sulfotransferase modulates α-dystroglycan glycosylation by 3-O-sulfation of glucuronic acid on matriglycan.					
32149355	4	14	theme	HNK-1ST/carbohydrate	593:612	arg1	sulfotransferase					575:590	HNK-1 sulfotransferase	569:590	HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase)	569:630	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	4	14	theme	HNK-1ST/carbohydrate	593:612	arg1	sulfotransferase					614:629	HNK-1ST/carbohydrate sulfotransferase	593:629	HNK-1ST/carbohydrate sulfotransferase	593:629	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	2	15	theme	glycan	368:373	arg1	-GlcA-β3-Xyl-α3-					422:437	-GlcA-β3-Xyl-α3-	422:437	-GlcA-β3-Xyl-α3-	422:437	Previously, we and others further elucidated the functional O-mannose glycan structure that is terminated by matriglycan, [(-GlcA-β3-Xyl-α3-)n].					
32149355	2	15	theme	glycan	368:373	arg1	structure					375:383	the functional O-mannose glycan structure	343:383	the functional O-mannose glycan structure that is terminated by matriglycan	343:417	Previously, we and others further elucidated the functional O-mannose glycan structure that is terminated by matriglycan, [(-GlcA-β3-Xyl-α3-)n].					
32149355	1	16	theme	muscular	234:241	arg1	dystrophies					243:253	congenital/limb-girdle muscular dystrophies	211:253	congenital/limb-girdle muscular dystrophies	211:253	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	0	17	theme	α-dystroglycan	33:46	arg1	glycosylation					48:60	α-dystroglycan glycosylation	33:60	α-dystroglycan glycosylation	33:60	HNK-1 sulfotransferase modulates α-dystroglycan glycosylation by 3-O-sulfation of glucuronic acid on matriglycan.					
32149355	2	18	theme	O-mannose	358:366	arg1	-GlcA-β3-Xyl-α3-					422:437	-GlcA-β3-Xyl-α3-	422:437	-GlcA-β3-Xyl-α3-	422:437	Previously, we and others further elucidated the functional O-mannose glycan structure that is terminated by matriglycan, [(-GlcA-β3-Xyl-α3-)n].					
32149355	2	18	theme	O-mannose	358:366	arg1	structure					375:383	the functional O-mannose glycan structure	343:383	the functional O-mannose glycan structure that is terminated by matriglycan	343:417	Previously, we and others further elucidated the functional O-mannose glycan structure that is terminated by matriglycan, [(-GlcA-β3-Xyl-α3-)n].					
32149355	5	19	theme	weight	935:940	arg1	resistant					1017:1025	resistant	1017:1025	resistant	1017:1025	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	5	19	theme	weight	935:940	arg1	α-dystroglycan					942:955	the functional, lower molecular weight α-dystroglycan	903:955	the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated,	903:1012	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	7	20	theme	matriglycan	1276:1286	arg1	GlcA					1268:1271	the nonreducing GlcA	1252:1271	the nonreducing GlcA of matriglycan	1252:1286	Our data strongly support a tissue specific mechanism in which HNK-1ST regulates polymer length by competing with LARGE for the 3-position on the nonreducing GlcA of matriglycan.					
32149355	7	21	theme	polymer	1191:1197	arg1	length					1199:1204	polymer length	1191:1204	polymer length	1191:1204	Our data strongly support a tissue specific mechanism in which HNK-1ST regulates polymer length by competing with LARGE for the 3-position on the nonreducing GlcA of matriglycan.					
32149355	0	22	from	matriglycan	101:111	arg1	3-O-sulfation					65:77	3-O-sulfation	65:77	3-O-sulfation of glucuronic acid on matriglycan	65:111	HNK-1 sulfotransferase modulates α-dystroglycan glycosylation by 3-O-sulfation of glucuronic acid on matriglycan.					
32149355	1	23	theme	multiple	127:134	arg1	genes					136:140	multiple genes	127:140	multiple genes required for proper O-mannosylation of α-dystroglycan	127:194	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	3	24	theme	repeating	448:456	arg1	receptor					483:490	a receptor	481:490	a receptor for proteins in the extracellular matrix	481:531	This repeating disaccharide serves as a receptor for proteins in the extracellular matrix.					
32149355	3	24	theme	repeating	448:456	arg1	disaccharide					458:469	This repeating disaccharide	443:469	This repeating disaccharide	443:469	This repeating disaccharide serves as a receptor for proteins in the extracellular matrix.					
32149355	4	25	theme	further	716:722	arg1	polymerization					736:749	further matriglycan polymerization	716:749	further matriglycan polymerization	716:749	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	5	26	theme	HNK-1ST	982:988	arg1	expression					990:999	HNK-1ST expression	982:999	HNK-1ST expression	982:999	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	1	27	theme	abnormal	259:266	arg1	development					274:284	abnormal brain development	259:284	abnormal brain development	259:284	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	5	28	theme	matriglycan	890:900	arg1	digestion					877:885	exoglycosidase digestion	862:885	exoglycosidase digestion of matriglycan	862:900	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	6	29	theme	cap	1051:1053	arg1	Removal					1028:1034	Removal	1028:1034	Removal of the sulfate cap by a sulfatase	1028:1068	Removal of the sulfate cap by a sulfatase facilitated dual-glycosidase digestion.					
32149355	5	30	theme	mouse	821:825	arg1	heart					827:831	mouse heart	821:831	mouse heart	821:831	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	0	31	from	acid	93:96	arg1	matriglycan					101:111	matriglycan	101:111	matriglycan	101:111	HNK-1 sulfotransferase modulates α-dystroglycan glycosylation by 3-O-sulfation of glucuronic acid on matriglycan.					
32149355	1	32	theme	brain	268:272	arg1	development					274:284	abnormal brain development	259:284	abnormal brain development	259:284	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	6	33	theme	sulfate	1043:1049	arg1	cap					1051:1053	the sulfate cap	1039:1053	the sulfate cap	1039:1053	Removal of the sulfate cap by a sulfatase facilitated dual-glycosidase digestion.					
32149355	7	34	from	3-position	1238:1247	arg1	GlcA					1268:1271	the nonreducing GlcA	1252:1271	the nonreducing GlcA of matriglycan	1252:1286	Our data strongly support a tissue specific mechanism in which HNK-1ST regulates polymer length by competing with LARGE for the 3-position on the nonreducing GlcA of matriglycan.					
32149355	3	35	from	proteins	496:503	arg1	matrix					526:531	the extracellular matrix	508:531	the extracellular matrix	508:531	This repeating disaccharide serves as a receptor for proteins in the extracellular matrix.					
32149355	1	36	gly	O-mannosylation	162:176	arg1	α-dystroglycan					181:194	α-dystroglycan	181:194	α-dystroglycan	181:194	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	1	37	from	dystrophies	243:253	arg1	mammals					289:295	mammals	289:295	mammals	289:295	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	4	38	theme	HNK-1	569:573	arg1	sulfotransferase					575:590	HNK-1 sulfotransferase	569:590	HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase)	569:630	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	4	38	theme	HNK-1	569:573	arg1	sulfotransferase					614:629	HNK-1ST/carbohydrate sulfotransferase	593:629	HNK-1ST/carbohydrate sulfotransferase	593:629	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	1	39	from	development	274:284	arg1	mammals					289:295	mammals	289:295	mammals	289:295	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	3	40	theme	extracellular	512:524	arg1	matrix					526:531	the extracellular matrix	508:531	the extracellular matrix	508:531	This repeating disaccharide serves as a receptor for proteins in the extracellular matrix.					
32149355	4	41	theme	matriglycan	673:683	arg1	matriglycan					673:683	matriglycan	673:683	matriglycan	673:683	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	4	41	theme	matriglycan	673:683	arg1	residues					661:668	terminal glucuronyl residues	641:668	terminal glucuronyl residues of matriglycan	641:683	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
32149355	7	42	theme	specific	1145:1152	arg1	mechanism					1154:1162	a tissue specific mechanism	1136:1162	a tissue specific mechanism in which HNK-1ST regulates polymer length by competing with LARGE for the 3-position on the nonreducing GlcA of matriglycan	1136:1286	Our data strongly support a tissue specific mechanism in which HNK-1ST regulates polymer length by competing with LARGE for the 3-position on the nonreducing GlcA of matriglycan.					
32149355	2	43	dep	-GlcA-β3-Xyl-α3-	422:437	arg1	n					439:439	n	439:439	n	439:439	Previously, we and others further elucidated the functional O-mannose glycan structure that is terminated by matriglycan, [(-GlcA-β3-Xyl-α3-)n].					
32149355	1	44	theme	proper	155:160	arg1	O-mannosylation					162:176	proper O-mannosylation	155:176	proper O-mannosylation of α-dystroglycan	155:194	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	0	45	theme	acid	93:96	arg1	3-O-sulfation					65:77	3-O-sulfation	65:77	3-O-sulfation of glucuronic acid on matriglycan	65:111	HNK-1 sulfotransferase modulates α-dystroglycan glycosylation by 3-O-sulfation of glucuronic acid on matriglycan.					
32149355	5	46	located	detected	957:964	arg2	resistant					1017:1025	resistant	1017:1025	resistant	1017:1025	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	5	46	located	detected	957:964	arg2	α-dystroglycan					942:955	the functional, lower molecular weight α-dystroglycan	903:955	the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated,	903:1012	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	5	46	located	detected	957:964	arg1	brain					969:973	brain	969:973	brain	969:973	While α-dystroglycan isolated from mouse heart and kidney is susceptible to exoglycosidase digestion of matriglycan, the functional, lower molecular weight α-dystroglycan detected in brain, where HNK-1ST expression is elevated, is resistant.					
32149355	1	47	from	Mutations	114:122	arg1	genes					136:140	multiple genes	127:140	multiple genes required for proper O-mannosylation of α-dystroglycan	127:194	Mutations in multiple genes required for proper O-mannosylation of α-dystroglycan are causal for congenital/limb-girdle muscular dystrophies and abnormal brain development in mammals.					
32149355	0	48	theme	glucuronic	82:91	arg1	acid					93:96	glucuronic acid	82:96	glucuronic acid on matriglycan	82:111	HNK-1 sulfotransferase modulates α-dystroglycan glycosylation by 3-O-sulfation of glucuronic acid on matriglycan.					
32149355	4	49	theme	LARGE1	758:763	arg1	glycosyltransferase					765:783	the LARGE1 glycosyltransferase	754:783	the LARGE1 glycosyltransferase	754:783	Here, we demonstrate in vitro that HNK-1 sulfotransferase (HNK-1ST/carbohydrate sulfotransferase) sulfates terminal glucuronyl residues of matriglycan at the 3-hydroxyl and prevents further matriglycan polymerization by the LARGE1 glycosyltransferase.					
33396096	0	0	theme	metal	82:86	arg1	environment					88:98	a toxic metal environment	74:98	a toxic metal environment	74:98	Production and functionality of exopolysaccharides in bacteria exposed to a toxic metal environment.					
33396096	0	1	from	functionality	15:27	arg1	bacteria					54:61	bacteria	54:61	bacteria	54:61	Production and functionality of exopolysaccharides in bacteria exposed to a toxic metal environment.					
33396096	4	2	theme	FT-IR	625:629	arg1	analysis					645:652	FT-IR, XRD, and SEM analysis	625:652	FT-IR, XRD, and SEM analysis	625:652	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	0	3	theme	toxic	76:80	arg1	environment					88:98	a toxic metal environment	74:98	a toxic metal environment	74:98	Production and functionality of exopolysaccharides in bacteria exposed to a toxic metal environment.					
33396096	7	4	theme	chelating	1215:1223	arg1	substances					1225:1234	chelating substances	1215:1234	chelating substances	1215:1234	Owing to good adsorbing nature, the exopolysaccharides could be used as chelating substances for wastewater treatment.					
33396096	7	4	theme	chelating	1215:1223	arg1	exopolysaccharides					1179:1196	the exopolysaccharides	1175:1196	the exopolysaccharides	1175:1196	Owing to good adsorbing nature, the exopolysaccharides could be used as chelating substances for wastewater treatment.					
33396096	2	5	theme	KMS3-1	351:356	arg1	strain					358:363	KMS3-1 strain	351:363	KMS3-1 strain	351:363	In addition, the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain was evaluated in a batch mode.					
33396096	0	6	from	Production	0:9	arg1	bacteria					54:61	bacteria	54:61	bacteria	54:61	Production and functionality of exopolysaccharides in bacteria exposed to a toxic metal environment.					
33396096	2	7	theme	adsorption	291:300	arg1	efficacy					302:309	the metal adsorption efficacy	281:309	the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain	281:363	In addition, the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain was evaluated in a batch mode.					
33396096	6	8	theme	XRD	1115:1117	arg1	analysis					1119:1126	XRD analysis	1115:1126	XRD analysis in this study	1115:1140	To the best of our knowledge, EPS demonstrating proficient metal adsorption was substantiated by XRD analysis in this study.					
33396096	1	9	dep	production	120:129	arg1	the					116:118	the	116:118	the	116:118	In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated.					
33396096	2	10	theme	metal	285:289	arg1	efficacy					302:309	the metal adsorption efficacy	281:309	the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain	281:363	In addition, the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain was evaluated in a batch mode.					
33396096	7	11	used	used	1207:1210	arg2	exopolysaccharides					1179:1196	the exopolysaccharides	1175:1196	the exopolysaccharides	1175:1196	Owing to good adsorbing nature, the exopolysaccharides could be used as chelating substances for wastewater treatment.					
33396096	7	11	used	used	1207:1210	arg2	substances					1225:1234	chelating substances	1215:1234	chelating substances	1215:1234	Owing to good adsorbing nature, the exopolysaccharides could be used as chelating substances for wastewater treatment.					
33396096	4	12	theme	XRD	632:634	arg1	analysis					645:652	FT-IR, XRD, and SEM analysis	625:652	FT-IR, XRD, and SEM analysis	625:652	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	1	13	theme	exopolysaccharides	161:178	arg1	production					120:129	production	120:129	production	120:129	In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated.					
33396096	1	13	theme	exopolysaccharides	161:178	arg1	analysis					149:156	compositional analysis	135:156	compositional analysis	135:156	In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated.					
33396096	6	14	from	analysis	1119:1126	arg1	study					1136:1140	this study	1131:1140	this study	1131:1140	To the best of our knowledge, EPS demonstrating proficient metal adsorption was substantiated by XRD analysis in this study.					
33396096	2	15	theme	batch	384:388	arg1	mode					390:393	a batch mode	382:393	a batch mode	382:393	In addition, the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain was evaluated in a batch mode.					
33396096	7	16	theme	good	1152:1155	arg1	nature					1167:1172	good adsorbing nature	1152:1172	good adsorbing nature	1152:1172	Owing to good adsorbing nature, the exopolysaccharides could be used as chelating substances for wastewater treatment.					
33396096	3	17	theme	exopolysaccharides	420:437	arg1	production					406:415	Increased production	396:415	Increased production of exopolysaccharides by KMS3-1 strain	396:454	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	7	18	theme	wastewater	1240:1249	arg1	treatment					1251:1259	wastewater treatment	1240:1259	wastewater treatment	1240:1259	Owing to good adsorbing nature, the exopolysaccharides could be used as chelating substances for wastewater treatment.					
33396096	2	19	theme	exopolysaccharides	314:331	arg1	efficacy					302:309	the metal adsorption efficacy	281:309	the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain	281:363	In addition, the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain was evaluated in a batch mode.					
33396096	1	20	theme	Bacillus	192:199	arg1	KMS3-1					208:213	Bacillus cereus KMS3-1	192:213	Bacillus cereus KMS3-1 grown in metal amended conditions	192:247	In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated.					
33396096	7	21	theme	adsorbing	1157:1165	arg1	nature					1167:1172	good adsorbing nature	1152:1172	good adsorbing nature	1152:1172	Owing to good adsorbing nature, the exopolysaccharides could be used as chelating substances for wastewater treatment.					
33396096	5	22	theme	maximum	889:895	arg1	54.05					974:978	54.05	974:978	54.05	974:978	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	5	22	theme	maximum	889:895	arg1	Qmax					918:921	Qmax	918:921	Qmax	918:921	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	5	22	theme	maximum	889:895	arg1	capacity					908:915	the maximum adsorption capacity	885:915	the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II)	885:960	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	5	23	theme	adsorption	897:906	arg1	54.05					974:978	54.05	974:978	54.05	974:978	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	5	23	theme	adsorption	897:906	arg1	Qmax					918:921	Qmax	918:921	Qmax	918:921	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	5	23	theme	adsorption	897:906	arg1	capacity					908:915	the maximum adsorption capacity	885:915	the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II)	885:960	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	4	24	theme	SEM	641:643	arg1	analysis					645:652	FT-IR, XRD, and SEM analysis	625:652	FT-IR, XRD, and SEM analysis	625:652	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	5	25	theme	equilibrium	816:826	arg1	results					828:834	equilibrium results	816:834	equilibrium results	816:834	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	4	26	dep	groups	730:735	arg1	O‒H					738:740	O‒H	738:740	O‒H	738:740	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	4	26	dep	groups	730:735	arg1	CH					743:744	CH	743:744	CH	743:744	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	4	26	dep	groups	730:735	arg1	C‒C˭O					761:765	C‒C˭O	761:765	C‒C˭O	761:765	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	4	26	dep	groups	730:735	arg1	groups					730:735	their functional groups	713:735	their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O)	713:766	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	4	26	dep	groups	730:735	arg1	C˭O					747:749	C˭O	747:749	C˭O	747:749	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	4	26	dep	groups	730:735	arg1	C‒O					752:754	C‒O	752:754	C‒O	752:754	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	6	27	theme	metal	1077:1081	arg1	adsorption					1083:1092	proficient metal adsorption	1066:1092	proficient metal adsorption	1066:1092	To the best of our knowledge, EPS demonstrating proficient metal adsorption was substantiated by XRD analysis in this study.					
33396096	4	28	theme	functional	719:728	arg1	O‒H					738:740	O‒H	738:740	O‒H	738:740	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	4	28	theme	functional	719:728	arg1	CH					743:744	CH	743:744	CH	743:744	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	4	28	theme	functional	719:728	arg1	C‒C˭O					761:765	C‒C˭O	761:765	C‒C˭O	761:765	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	4	28	theme	functional	719:728	arg1	groups					730:735	their functional groups	713:735	their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O)	713:766	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	4	28	theme	functional	719:728	arg1	C˭O					747:749	C˭O	747:749	C˭O	747:749	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	4	28	theme	functional	719:728	arg1	C‒O					752:754	C‒O	752:754	C‒O	752:754	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	0	29	theme	exopolysaccharides	32:49	arg1	functionality					15:27	functionality	15:27	functionality	15:27	Production and functionality of exopolysaccharides in bacteria exposed to a toxic metal environment.					
33396096	0	29	theme	exopolysaccharides	32:49	arg1	Production					0:9	Production	0:9	Production	0:9	Production and functionality of exopolysaccharides in bacteria exposed to a toxic metal environment.					
33396096	4	30	theme	detoxification	783:796	arg1	process					798:804	the detoxification process	779:804	the detoxification process	779:804	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	5	31	theme	EPS	927:929	arg1	54.05					974:978	54.05	974:978	54.05	974:978	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	5	31	theme	EPS	927:929	arg1	Qmax					918:921	Qmax	918:921	Qmax	918:921	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	5	31	theme	EPS	927:929	arg1	capacity					908:915	the maximum adsorption capacity	885:915	the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II)	885:960	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	1	32	dep	Bacillus	192:199	arg1	cereus					201:206	cereus	201:206	cereus	201:206	In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated.					
33396096	3	33	theme	KMS3-1	442:447	arg1	strain					449:454	KMS3-1 strain	442:454	KMS3-1 strain	442:454	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	3	34	theme	amended	495:501	arg1	100 mg/L					515:522	100 mg/L	515:522	100 mg/L	515:522	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	3	34	theme	amended	495:501	arg1	conditions					503:512	metal amended conditions	489:512	metal amended conditions (100 mg/L)	489:523	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	3	35	theme	Increased	396:404	arg1	production					406:415	Increased production	396:415	Increased production of exopolysaccharides by KMS3-1 strain	396:454	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	3	36	theme	>Cd	578:580	arg1	>Control					585:592	Pb(II)>Cu(II)>Cd(II)>Control	565:592	Pb(II)>Cu(II)>Cd(II)>Control	565:592	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	3	37	theme	Pb	565:566	arg1	>Control					585:592	Pb(II)>Cu(II)>Cd(II)>Control	565:592	Pb(II)>Cu(II)>Cd(II)>Control	565:592	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	4	38	theme	metal	694:698	arg1	ions					700:703	metal ions	694:703	metal ions	694:703	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	6	39	theme	proficient	1066:1075	arg1	adsorption					1083:1092	proficient metal adsorption	1066:1092	proficient metal adsorption	1066:1092	To the best of our knowledge, EPS demonstrating proficient metal adsorption was substantiated by XRD analysis in this study.					
33396096	1	40	theme	metal	224:228	arg1	conditions					238:247	metal amended conditions	224:247	metal amended conditions	224:247	In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated.					
33396096	5	41	theme	Langmuir	857:864	arg1	model					866:870	the Langmuir model	853:870	the Langmuir model	853:870	Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively.					
33396096	3	42	theme	metal	489:493	arg1	100 mg/L					515:522	100 mg/L	515:522	100 mg/L	515:522	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	3	42	theme	metal	489:493	arg1	conditions					503:512	metal amended conditions	489:512	metal amended conditions (100 mg/L)	489:523	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	1	43	theme	amended	230:236	arg1	conditions					238:247	metal amended conditions	224:247	metal amended conditions	224:247	In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated.					
33396096	3	44	theme	>Control	585:592	arg1	order					556:560	the order	552:560	the order of Pb(II)>Cu(II)>Cd(II)>Control	552:592	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	4	45	theme	EPS	615:617	arg1	Characterization					595:610	Characterization	595:610	Characterization of EPS using FT-IR, XRD, and SEM analysis	595:652	Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process.					
33396096	3	46	theme	>Cu	571:573	arg1	>Control					585:592	Pb(II)>Cu(II)>Cd(II)>Control	565:592	Pb(II)>Cu(II)>Cd(II)>Control	565:592	Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control.					
33396096	1	47	theme	compositional	135:147	arg1	analysis					149:156	compositional analysis	135:156	compositional analysis	135:156	In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated.					
32770134	5	0	dep	development	730:740	arg1	the					726:728	the	726:728	the	726:728	We describe the development and application of a chemical method for producing oligosaccharides from polysaccharides.					
32770134	8	1	theme	food	1128:1131	arg1	compositions					1112:1123	the polysaccharide compositions	1093:1123	the polysaccharide compositions of food and feces	1093:1141	Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies.					
32770134	0	2	theme	structural	81:90	arg1	analysis					92:99	structural analysis	81:99	structural analysis	81:99	A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis.					
32770134	6	3	theme	advanced	883:890	arg1	methods					940:946	advanced liquid chromatography-mass spectrometry (LC-MS) methods	883:946	advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput	883:993	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	8	4	theme	feces	1137:1141	arg1	compositions					1112:1123	the polysaccharide compositions	1093:1123	the polysaccharide compositions of food and feces	1093:1141	Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies.					
32770134	4	5	contain	have	561:564	arg2	analysis					583:590	advanced genomic analysis	566:590	advanced genomic analysis	566:590	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	4	5	contain	have	561:564	arg1	enzymes					553:559	restriction enzymes	541:559	restriction enzymes	541:559	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	4	6	theme	proteomic	618:626	arg1	analysis					628:635	advanced proteomic analysis	609:635	advanced proteomic analysis	609:635	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	5	7	theme	chemical	763:770	arg1	method					772:777	a chemical method	761:777	a chemical method for producing oligosaccharides from polysaccharides	761:829	We describe the development and application of a chemical method for producing oligosaccharides from polysaccharides.					
32770134	6	8	with	methods	940:946	arg1	throughput					984:993	throughput	984:993	throughput	984:993	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	6	8	with	methods	940:946	arg1	accuracy					971:978	accuracy	971:978	accuracy	971:978	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	6	8	with	methods	940:946	arg1	sensitivity					958:968	high sensitivity	953:968	high sensitivity	953:968	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	4	9	theme	advanced	609:616	arg1	analysis					628:635	advanced proteomic analysis	609:635	advanced proteomic analysis	609:635	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	5	10	theme	method	772:777	arg1	application					746:756	application	746:756	application	746:756	We describe the development and application of a chemical method for producing oligosaccharides from polysaccharides.					
32770134	5	10	theme	method	772:777	arg1	development					730:740	development	730:740	development	730:740	We describe the development and application of a chemical method for producing oligosaccharides from polysaccharides.					
32770134	4	11	theme	universal	678:686	arg1	digestion					703:711	universal polysaccharide digestion	678:711	universal polysaccharide digestion	678:711	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	1	12	from	biomolecules	140:151	arg1	nature					156:161	nature	156:161	nature	156:161	Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions.					
32770134	0	13	theme	nonenzymatic	2:13	arg1	method					15:20	A nonenzymatic method	0:20	A nonenzymatic method for cleaving polysaccharides	0:49	A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis.					
32770134	6	14	theme	high	953:956	arg1	sensitivity					958:968	high sensitivity	953:968	high sensitivity	953:968	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	6	15	theme	released	836:843	arg1	oligosaccharides					845:860	The released oligosaccharides	832:860	The released oligosaccharides	832:860	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	3	16	theme	mass	440:443	arg1	techniques					458:467	mass spectrometry techniques	440:467	mass spectrometry techniques	440:467	Furthermore, large polymeric structures cannot be directly analyzed by mass spectrometry techniques, a problem that has been solved for polynucleotides and proteins.					
32770134	3	16	theme	mass	440:443	arg1	problem					472:478	a problem	470:478	a problem that has been solved for polynucleotides and proteins	470:532	Furthermore, large polymeric structures cannot be directly analyzed by mass spectrometry techniques, a problem that has been solved for polynucleotides and proteins.					
32770134	6	17	theme	liquid	892:897	arg1	spectrometry					919:930	liquid chromatography-mass spectrometry	892:930	advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput	883:993	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	6	17	theme	liquid	892:897	arg1	LC-MS					933:937	LC-MS	933:937	LC-MS	933:937	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	4	18	theme	polysaccharide	688:701	arg1	digestion					703:711	universal polysaccharide digestion	678:711	universal polysaccharide digestion	678:711	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	3	19	theme	large	382:386	arg1	structures					398:407	large polymeric structures	382:407	large polymeric structures	382:407	Furthermore, large polymeric structures cannot be directly analyzed by mass spectrometry techniques, a problem that has been solved for polynucleotides and proteins.					
32770134	4	20	theme	genomic	575:581	arg1	analysis					583:590	advanced genomic analysis	566:590	advanced genomic analysis	566:590	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	8	21	theme	polysaccharide	1097:1110	arg1	compositions					1112:1123	the polysaccharide compositions	1093:1123	the polysaccharide compositions of food and feces	1093:1141	Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies.					
32770134	3	22	theme	polymeric	388:396	arg1	structures					398:407	large polymeric structures	382:407	large polymeric structures	382:407	Furthermore, large polymeric structures cannot be directly analyzed by mass spectrometry techniques, a problem that has been solved for polynucleotides and proteins.					
32770134	7	23	used	used	1019:1022	arg2	technique					1000:1008	The technique	996:1008	The technique	996:1008	The technique is first used to identify polysaccharides by oligosaccharide fingerprinting.					
32770134	3	24	theme	spectrometry	445:456	arg1	techniques					458:467	mass spectrometry techniques	440:467	mass spectrometry techniques	440:467	Furthermore, large polymeric structures cannot be directly analyzed by mass spectrometry techniques, a problem that has been solved for polynucleotides and proteins.					
32770134	3	24	theme	spectrometry	445:456	arg1	problem					472:478	a problem	470:478	a problem that has been solved for polynucleotides and proteins	470:532	Furthermore, large polymeric structures cannot be directly analyzed by mass spectrometry techniques, a problem that has been solved for polynucleotides and proteins.					
32770134	0	25	theme	cleaving	26:33	arg1	polysaccharides					35:49	cleaving polysaccharides	26:49	cleaving polysaccharides	26:49	A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis.					
32770134	4	26	theme	equivalent	656:665	arg1	enzyme					667:672	no equivalent enzyme	653:672	no equivalent enzyme for universal polysaccharide digestion	653:711	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	4	27	theme	restriction	541:551	arg1	enzymes					553:559	restriction enzymes	541:559	restriction enzymes	541:559	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	2	28	theme	uniform	350:356	arg1	structure					358:366	a uniform structure	348:366	a uniform structure	348:366	Polysaccharides cannot be simply sequenced because they are often highly branched and lack a uniform structure.					
32770134	6	29	theme	spectrometry	919:930	arg1	methods					940:946	advanced liquid chromatography-mass spectrometry (LC-MS) methods	883:946	advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput	883:993	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	4	30	theme	advanced	566:573	arg1	analysis					583:590	advanced genomic analysis	566:590	advanced genomic analysis	566:590	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	8	31	theme	technique	1195:1203	arg1	utility					1184:1190	the utility	1180:1190	the utility of technique in food and clinical studies	1180:1232	Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies.					
32770134	4	32	contain	has	605:607	arg1	trypsin					597:603	trypsin	597:603	trypsin	597:603	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	4	32	contain	has	605:607	arg2	analysis					628:635	advanced proteomic analysis	609:635	advanced proteomic analysis	609:635	While restriction enzymes have advanced genomic analysis, and trypsin has advanced proteomic analysis, there has been no equivalent enzyme for universal polysaccharide digestion.					
32770134	1	33	theme	abundant	131:138	arg1	biomolecules					140:151	the most abundant biomolecules	122:151	the most abundant biomolecules in nature	122:161	Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions.					
32770134	1	33	theme	abundant	131:138	arg1	Polysaccharides					102:116	Polysaccharides	102:116	Polysaccharides	102:116	Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions.					
32770134	5	34	from	polysaccharides	815:829	arg1	oligosaccharides					793:808	oligosaccharides	793:808	oligosaccharides from polysaccharides	793:829	We describe the development and application of a chemical method for producing oligosaccharides from polysaccharides.					
32770134	1	35	theme	structures	220:229	arg1	terms					196:200	terms	196:200	terms of their chemical structures and biological functions	196:254	Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions.					
32770134	6	36	theme	chromatography-mass	899:917	arg1	spectrometry					919:930	liquid chromatography-mass spectrometry	892:930	advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput	883:993	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	6	36	theme	chromatography-mass	899:917	arg1	LC-MS					933:937	LC-MS	933:937	LC-MS	933:937	The released oligosaccharides are characterized by advanced liquid chromatography-mass spectrometry (LC-MS) methods with high sensitivity, accuracy and throughput.					
32770134	8	37	from	utility	1184:1190	arg1	food					1208:1211	food	1208:1211	food	1208:1211	Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies.					
32770134	8	37	from	utility	1184:1190	arg1	studies					1226:1232	clinical studies	1217:1232	clinical studies	1217:1232	Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies.					
32770134	1	38	theme	chemical	211:218	arg1	structures					220:229	chemical structures	211:229	chemical structures	211:229	Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions.					
32770134	1	39	theme	biological	235:244	arg1	functions					246:254	biological functions	235:254	biological functions	235:254	Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions.					
32770134	8	40	theme	clinical	1217:1224	arg1	studies					1226:1232	clinical studies	1217:1232	clinical studies	1217:1232	Next, the polysaccharide compositions of food and feces are determined, further illustrating the utility of technique in food and clinical studies.					
32770134	7	41	theme	oligosaccharide	1055:1069	arg1	fingerprinting					1071:1084	oligosaccharide fingerprinting	1055:1084	oligosaccharide fingerprinting	1055:1084	The technique is first used to identify polysaccharides by oligosaccharide fingerprinting.					
32770134	1	42	theme	functions	246:254	arg1	terms					196:200	terms	196:200	terms of their chemical structures and biological functions	196:254	Polysaccharides are the most abundant biomolecules in nature, but are the least understood in terms of their chemical structures and biological functions.					
32648306	4	0	theme	in	733:734	arg1	effects					741:747	the resultant in vivo effects	719:747	the resultant in vivo effects on a rat model of acute inflammation	719:784	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	3	1	theme	mannose/glucose-specific	526:549	arg1	lectin					551:556	a ConA-like mannose/glucose-specific lectin	514:556	a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds	514:589	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	3	1	theme	mannose/glucose-specific	526:549	arg1	CaBo					592:595	CaBo	592:595	CaBo	592:595	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	3	2	from	seeds	585:589	arg1	lectin					551:556	a ConA-like mannose/glucose-specific lectin	514:556	a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds	514:589	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	3	2	from	seeds	585:589	arg1	study					505:509	a structural and biological study	477:509	a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo	477:595	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	3	2	from	seeds	585:589	arg1	CaBo					592:595	CaBo	592:595	CaBo	592:595	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	5	3	theme	virtual	789:795	arg1	screening					797:805	A virtual screening	787:805	A virtual screening	787:805	A virtual screening was also carried out to cover a larger number of possible bindings of CaBo.					
32648306	3	4	theme	lectin	551:556	arg1	study					505:509	a structural and biological study	477:509	a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo	477:595	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	4	5	from	binding	633:639	arg1	model					758:762	a rat model	752:762	a rat model of acute inflammation	752:784	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	7	6	theme	glycosylated	1339:1350	arg1	receptors					1352:1360	cell surface glycosylated receptors	1326:1360	cell surface glycosylated receptors	1326:1360	These results confirm the ability of CaBo to interact with hybrid and high-mannose N-glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors.					
32648306	6	7	with	rats	1032:1035	arg1	participation					1046:1058	the participation	1042:1058	the participation of the carbohydrate recognition domain (CRD) and histamine release	1042:1125	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	4	8	dep	in	733:734	arg1	vivo					736:739	vivo	736:739	vivo	736:739	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	7	9	dep	glycosylated	1339:1350	arg1	surface					1331:1337	cell surface	1326:1337	cell surface	1326:1337	These results confirm the ability of CaBo to interact with hybrid and high-mannose N-glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors.					
32648306	7	10	theme	high-mannose	1198:1209	arg1	N-glycans					1211:1219	hybrid and high-mannose N-glycans	1187:1219	hybrid and high-mannose N-glycans	1187:1219	These results confirm the ability of CaBo to interact with hybrid and high-mannose N-glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors.					
32648306	4	11	theme	inflammation	773:784	arg1	model					758:762	a rat model	752:762	a rat model of acute inflammation	752:784	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	6	12	theme	histamine	1109:1117	arg1	release					1119:1125	histamine release	1109:1125	histamine release	1109:1125	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	4	13	theme	resultant	723:731	arg1	effects					741:747	the resultant in vivo effects	719:747	the resultant in vivo effects on a rat model of acute inflammation	719:784	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	4	14	theme	rat	754:756	arg1	model					758:762	a rat model	752:762	a rat model of acute inflammation	752:784	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	3	15	theme	bonariensis	573:583	arg1	seeds					585:589	Canavalia bonariensis seeds	563:589	Canavalia bonariensis seeds	563:589	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	6	16	theme	carbohydrate	1067:1078	arg1	CRD					1100:1102	CRD	1100:1102	CRD	1100:1102	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	6	16	theme	carbohydrate	1067:1078	arg1	domain					1092:1097	the carbohydrate recognition domain	1063:1097	the carbohydrate recognition domain (CRD)	1063:1103	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	5	17	theme	bindings	865:872	arg1	number					846:851	a larger number	837:851	a larger number of possible bindings of CaBo	837:880	A virtual screening was also carried out to cover a larger number of possible bindings of CaBo.					
32648306	7	18	theme	hybrid	1187:1192	arg1	N-glycans					1211:1219	hybrid and high-mannose N-glycans	1187:1219	hybrid and high-mannose N-glycans	1187:1219	These results confirm the ability of CaBo to interact with hybrid and high-mannose N-glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors.					
32648306	6	19	with	interaction	937:947	arg1	ligands					967:973	mannose-type ligands	954:973	mannose-type ligands	954:973	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	3	20	from	study	505:509	arg1	seeds					585:589	Canavalia bonariensis seeds	563:589	Canavalia bonariensis seeds	563:589	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	2	21	theme	glycocode	420:428	arg1	decoding					430:437	glycocode decoding	420:437	glycocode decoding	420:437	Researchers have been intrigued by oligosaccharides and glycoconjugates for their involvement as mediators of complex cellular events and then many biotechnological applications of lectins are based on glycocode decoding and their activities.					
32648306	6	22	theme	In	883:884	arg1	analysis					893:900	In silico analysis	883:900	In silico analysis	883:900	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	2	23	theme	events	345:350	arg1	mediators					315:323	mediators	315:323	mediators of complex cellular events	315:350	Researchers have been intrigued by oligosaccharides and glycoconjugates for their involvement as mediators of complex cellular events and then many biotechnological applications of lectins are based on glycocode decoding and their activities.					
32648306	5	24	theme	CaBo	877:880	arg1	bindings					865:872	possible bindings	856:872	possible bindings of CaBo	856:880	A virtual screening was also carried out to cover a larger number of possible bindings of CaBo.					
32648306	2	25	theme	cellular	336:343	arg1	events					345:350	complex cellular events	328:350	complex cellular events	328:350	Researchers have been intrigued by oligosaccharides and glycoconjugates for their involvement as mediators of complex cellular events and then many biotechnological applications of lectins are based on glycocode decoding and their activities.					
32648306	2	26	theme	lectins	399:405	arg1	applications					383:394	then many biotechnological applications	356:394	then many biotechnological applications of lectins	356:405	Researchers have been intrigued by oligosaccharides and glycoconjugates for their involvement as mediators of complex cellular events and then many biotechnological applications of lectins are based on glycocode decoding and their activities.					
32648306	2	27	theme	complex	328:334	arg1	events					345:350	complex cellular events	328:350	complex cellular events	328:350	Researchers have been intrigued by oligosaccharides and glycoconjugates for their involvement as mediators of complex cellular events and then many biotechnological applications of lectins are based on glycocode decoding and their activities.					
32648306	5	28	theme	larger	839:844	arg1	number					846:851	a larger number	837:851	a larger number of possible bindings of CaBo	837:880	A virtual screening was also carried out to cover a larger number of possible bindings of CaBo.					
32648306	0	29	theme	carbohydrate-binding	14:33	arg1	ability					35:41	the carbohydrate-binding ability	10:41	the carbohydrate-binding ability of Canavalia bonariensis lectin in inflammation models	10:96	Exploring the carbohydrate-binding ability of Canavalia bonariensis lectin in inflammation models.					
32648306	3	30	theme	ConA-like	516:524	arg1	lectin					551:556	a ConA-like mannose/glucose-specific lectin	514:556	a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds	514:589	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	3	30	theme	ConA-like	516:524	arg1	CaBo					592:595	CaBo	592:595	CaBo	592:595	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	3	31	theme	biological	494:503	arg1	study					505:509	a structural and biological study	477:509	a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo	477:595	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	0	32	theme	Canavalia	46:54	arg1	lectin					68:73	Canavalia bonariensis lectin	46:73	Canavalia bonariensis lectin	46:73	Exploring the carbohydrate-binding ability of Canavalia bonariensis lectin in inflammation models.					
32648306	2	33	theme	many	361:364	arg1	applications					383:394	then many biotechnological applications	356:394	then many biotechnological applications of lectins	356:405	Researchers have been intrigued by oligosaccharides and glycoconjugates for their involvement as mediators of complex cellular events and then many biotechnological applications of lectins are based on glycocode decoding and their activities.					
32648306	7	34	with	interaction	1309:1319	arg1	receptors					1352:1360	cell surface glycosylated receptors	1326:1360	cell surface glycosylated receptors	1326:1360	These results confirm the ability of CaBo to interact with hybrid and high-mannose N-glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors.					
32648306	6	35	theme	domain	1092:1097	arg1	participation					1046:1058	the participation	1042:1058	the participation of the carbohydrate recognition domain (CRD) and histamine release	1042:1125	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	7	36	theme	cell	1326:1329	arg1	surface					1331:1337	cell surface	1326:1337	cell surface	1326:1337	These results confirm the ability of CaBo to interact with hybrid and high-mannose N-glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors.					
32648306	3	37	theme	structural	479:488	arg1	study					505:509	a structural and biological study	477:509	a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo	477:595	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	0	38	theme	lectin	68:73	arg1	ability					35:41	the carbohydrate-binding ability	10:41	the carbohydrate-binding ability of Canavalia bonariensis lectin in inflammation models	10:96	Exploring the carbohydrate-binding ability of Canavalia bonariensis lectin in inflammation models.					
32648306	6	39	theme	mannose-type	954:965	arg1	ligands					967:973	mannose-type ligands	954:973	mannose-type ligands	954:973	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	4	40	with	CaBo	644:647	arg1	M13					710:712	M13	710:712	M13	710:712	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	4	40	with	CaBo	644:647	arg1	MMA					676:678	MMA	676:678	MMA	676:678	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	4	40	with	CaBo	644:647	arg1	mannose-1,3-α-D-mannose					685:707	mannose-1,3-α-D-mannose	685:707	mannose-1,3-α-D-mannose (M13)	685:713	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	4	40	with	CaBo	644:647	arg1	α-methyl-D-mannoside					654:673	α-methyl-D-mannoside	654:673	α-methyl-D-mannoside (MMA)	654:679	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	2	41	theme	biotechnological	366:381	arg1	applications					383:394	then many biotechnological applications	356:394	then many biotechnological applications of lectins	356:405	Researchers have been intrigued by oligosaccharides and glycoconjugates for their involvement as mediators of complex cellular events and then many biotechnological applications of lectins are based on glycocode decoding and their activities.					
32648306	0	42	theme	bonariensis	56:66	arg1	lectin					68:73	Canavalia bonariensis lectin	46:73	Canavalia bonariensis lectin	46:73	Exploring the carbohydrate-binding ability of Canavalia bonariensis lectin in inflammation models.					
32648306	6	43	theme	CaBo	932:935	arg1	interaction					937:947	CaBo interaction	932:947	CaBo interaction with mannose-type ligands	932:973	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	7	44	gly	glycosylated	1339:1350	arg1	receptors					1352:1360	cell surface glycosylated receptors	1326:1360	cell surface glycosylated receptors	1326:1360	These results confirm the ability of CaBo to interact with hybrid and high-mannose N-glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors.					
32648306	1	45	theme	proteins	122:129	arg1	proteins					122:129	proteins	122:129	proteins of non-immune origin recognized for their ability to bind reversibly to carbohydrates	122:215	Lectins are a group of proteins of non-immune origin recognized for their ability to bind reversibly to carbohydrates.					
32648306	1	45	theme	proteins	122:129	arg1	Lectins					99:105	Lectins	99:105	Lectins	99:105	Lectins are a group of proteins of non-immune origin recognized for their ability to bind reversibly to carbohydrates.					
32648306	1	45	theme	proteins	122:129	arg1	group					113:117	a group	111:117	a group of proteins of non-immune origin recognized for their ability to bind reversibly to carbohydrates	111:215	Lectins are a group of proteins of non-immune origin recognized for their ability to bind reversibly to carbohydrates.					
32648306	6	46	theme	interaction	937:947	arg1	stability					919:927	the stability	915:927	the stability of CaBo interaction with mannose-type ligands	915:973	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	6	47	theme	release	1119:1125	arg1	participation					1046:1058	the participation	1042:1058	the participation of the carbohydrate recognition domain (CRD) and histamine release	1042:1125	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	4	48	theme	acute	767:771	arg1	inflammation					773:784	acute inflammation	767:784	acute inflammation	767:784	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	4	49	theme	CaBo	644:647	arg1	effects					741:747	the resultant in vivo effects	719:747	the resultant in vivo effects on a rat model of acute inflammation	719:784	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	4	49	theme	CaBo	644:647	arg1	binding					633:639	the binding	629:639	the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13)	629:713	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	1	50	theme	non-immune	134:143	arg1	origin					145:150	non-immune origin	134:150	non-immune origin recognized for their ability to bind reversibly to carbohydrates	134:215	Lectins are a group of proteins of non-immune origin recognized for their ability to bind reversibly to carbohydrates.					
32648306	4	51	from	effects	741:747	arg1	model					758:762	a rat model	752:762	a rat model of acute inflammation	752:784	More specifically, we evaluate the binding of CaBo with α-methyl-D-mannoside (MMA) and mannose-1,3-α-D-mannose (M13) and the resultant in vivo effects on a rat model of acute inflammation.					
32648306	6	52	dep	In	883:884	arg1	silico					886:891	silico	886:891	silico	886:891	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	0	53	theme	inflammation	78:89	arg1	models					91:96	inflammation models	78:96	inflammation models	78:96	Exploring the carbohydrate-binding ability of Canavalia bonariensis lectin in inflammation models.					
32648306	5	54	theme	possible	856:863	arg1	bindings					865:872	possible bindings	856:872	possible bindings of CaBo	856:880	A virtual screening was also carried out to cover a larger number of possible bindings of CaBo.					
32648306	1	55	theme	origin	145:150	arg1	proteins					122:129	proteins	122:129	proteins of non-immune origin recognized for their ability to bind reversibly to carbohydrates	122:215	Lectins are a group of proteins of non-immune origin recognized for their ability to bind reversibly to carbohydrates.					
32648306	6	56	theme	recognition	1080:1090	arg1	CRD					1100:1102	CRD	1100:1102	CRD	1100:1102	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	6	56	theme	recognition	1080:1090	arg1	domain					1092:1097	the carbohydrate recognition domain	1063:1097	the carbohydrate recognition domain (CRD)	1063:1103	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
32648306	0	57	from	ability	35:41	arg1	models					91:96	inflammation models	78:96	inflammation models	78:96	Exploring the carbohydrate-binding ability of Canavalia bonariensis lectin in inflammation models.					
32648306	7	58	theme	CaBo	1165:1168	arg1	ability					1154:1160	the ability	1150:1160	the ability of CaBo to interact with hybrid and high-mannose N-glycans	1150:1219	These results confirm the ability of CaBo to interact with hybrid and high-mannose N-glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors.					
32648306	7	59	theme	biological	1260:1269	arg1	activity					1271:1278	CaBo's biological activity	1253:1278	CaBo's biological activity	1253:1278	These results confirm the ability of CaBo to interact with hybrid and high-mannose N-glycans, supporting the hypothesis that CaBo's biological activity occurs primarily through its interaction with cell surface glycosylated receptors.					
32648306	3	60	theme	Canavalia	563:571	arg1	seeds					585:589	Canavalia bonariensis seeds	563:589	Canavalia bonariensis seeds	563:589	Here, we report a structural and biological study of a ConA-like mannose/glucose-specific lectin from Canavalia bonariensis seeds, CaBo.					
32648306	6	61	theme	acute	1010:1014	arg1	inflammation					1016:1027	acute inflammation	1010:1027	acute inflammation	1010:1027	In silico analysis demonstrated the stability of CaBo interaction with mannose-type ligands, and the lectin was able to induce acute inflammation in rats with the participation of the carbohydrate recognition domain (CRD) and histamine release.					
34850523	4	0	theme	gene	899:902	arg1	tool					912:915	the CRISPR/Cas9 gene editing tool	883:915	the CRISPR/Cas9 gene editing tool	883:915	Importantly, they are not cytotoxic and retain inhibitory activity in cells lacking elongated O-glycans, which are eliminated by the CRISPR/Cas9 gene editing tool.					
34850523	4	1	from	activity	812:819	arg1	cells					824:828	cells	824:828	cells lacking elongated O-glycans, which are eliminated by the CRISPR/Cas9 gene editing tool	824:915	Importantly, they are not cytotoxic and retain inhibitory activity in cells lacking elongated O-glycans, which are eliminated by the CRISPR/Cas9 gene editing tool.					
34850523	2	2	theme	new	370:372	arg1	1-4					386:388	1-4	386:388	1-4	386:388	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	2	theme	new	370:372	arg1	glycerates					374:383	four new glycerates	365:383	four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant	365:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	3	theme	GalNAc-T2	478:486	arg1	array					321:325	an array	318:325	an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant	318:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	3	theme	GalNAc-T2	478:486	arg1	inhibitors					464:473	active inhibitors	457:473	active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay	457:585	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	4	4	theme	CRISPR/Cas9	887:897	arg1	tool					912:915	the CRISPR/Cas9 gene editing tool	883:915	the CRISPR/Cas9 gene editing tool	883:915	Importantly, they are not cytotoxic and retain inhibitory activity in cells lacking elongated O-glycans, which are eliminated by the CRISPR/Cas9 gene editing tool.					
34850523	5	5	theme	relationship	939:950	arg1	study					952:956	A structure-activity relationship study	918:956	A structure-activity relationship study	918:956	A structure-activity relationship study unveiled a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition.					
34850523	3	6	link	O-linked	667:674	arg1	type					725:728	the other type	715:728	the other type of glycosyltransferase	715:751	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	3	6	link	O-linked	667:674	arg1	OGT					709:711	OGT	709:711	OGT	709:711	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	3	6	link	O-linked	667:674	arg1	transferase					696:706	O-linked N-acetylglucosamine transferase	667:706	O-linked N-acetylglucosamine transferase (OGT)	667:712	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	5	7	theme	conjugate	1000:1008	arg1	pharmacophore					1010:1022	a novel quinic acid-caffeic acid conjugate pharmacophore	967:1022	a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition	967:1046	A structure-activity relationship study unveiled a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition.					
34850523	5	8	theme	quinic	975:980	arg1	pharmacophore					1010:1022	a novel quinic acid-caffeic acid conjugate pharmacophore	967:1022	a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition	967:1046	A structure-activity relationship study unveiled a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition.					
34850523	4	9	theme	editing	904:910	arg1	tool					912:915	the CRISPR/Cas9 gene editing tool	883:915	the CRISPR/Cas9 gene editing tool	883:915	Importantly, they are not cytotoxic and retain inhibitory activity in cells lacking elongated O-glycans, which are eliminated by the CRISPR/Cas9 gene editing tool.					
34850523	5	10	theme	structure-activity	920:937	arg1	relationship					939:950	A structure-activity relationship	918:950	A structure-activity relationship study	918:956	A structure-activity relationship study unveiled a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition.					
34850523	1	11	theme	human	140:144	arg1	GalNAc-Ts					195:203	GalNAc-Ts	195:203	GalNAc-Ts	195:203	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	1	11	theme	human	140:144	arg1	transferases					181:192	human polypeptide N-acetylgalactosaminyl transferases	140:192	human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts)	140:204	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	0	12	from	farfara	44:50	arg1	Glycerates					18:27	Novel Quinic Acid Glycerates	0:27	Novel Quinic Acid Glycerates from Tussilago farfara	0:50	Novel Quinic Acid Glycerates from Tussilago farfara Inhibit Polypeptide GalNAc-Transferase.					
34850523	4	13	theme	inhibitory	801:810	arg1	activity					812:819	inhibitory activity	801:819	inhibitory activity in cells lacking elongated O-glycans, which are eliminated by the CRISPR/Cas9 gene editing tool	801:915	Importantly, they are not cytotoxic and retain inhibitory activity in cells lacking elongated O-glycans, which are eliminated by the CRISPR/Cas9 gene editing tool.					
34850523	2	14	theme	screening	505:513	arg1	approach					515:522	a combined screening approach	494:522	a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay	494:585	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	15	with	approach	515:522	arg1	sensor					554:559	a cell-based T2-specific sensor	529:559	a cell-based T2-specific sensor	529:559	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	15	with	approach	515:522	arg1	assay					581:585	purified enzyme assay	565:585	purified enzyme assay	565:585	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	6	16	theme	inhibitor	1136:1144	arg1	tool					1146:1149	an inhibitor tool	1133:1149	an inhibitor tool for GalNAc-T2	1133:1163	Overall, these new natural product inhibitors could serve as a basis for developing an inhibitor tool for GalNAc-T2.					
34850523	0	17	theme	Quinic	6:11	arg1	Glycerates					18:27	Novel Quinic Acid Glycerates	0:27	Novel Quinic Acid Glycerates from Tussilago farfara	0:50	Novel Quinic Acid Glycerates from Tussilago farfara Inhibit Polypeptide GalNAc-Transferase.					
34850523	3	18	theme	glycosyltransferase	733:751	arg1	type					725:728	the other type	715:728	the other type of glycosyltransferase	715:751	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	3	18	theme	glycosyltransferase	733:751	arg1	transferase					696:706	O-linked N-acetylglucosamine transferase	667:706	O-linked N-acetylglucosamine transferase (OGT)	667:712	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	2	19	theme	quinic	330:335	arg1	glycerates					374:383	four new glycerates	365:383	four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant	365:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	19	theme	quinic	330:335	arg1	derivatives					342:352	quinic acid derivatives	330:352	quinic acid derivatives	330:352	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	0	20	theme	Novel	0:4	arg1	Glycerates					18:27	Novel Quinic Acid Glycerates	0:27	Novel Quinic Acid Glycerates from Tussilago farfara	0:50	Novel Quinic Acid Glycerates from Tussilago farfara Inhibit Polypeptide GalNAc-Transferase.					
34850523	5	21	theme	acid-caffeic	982:993	arg1	pharmacophore					1010:1022	a novel quinic acid-caffeic acid conjugate pharmacophore	967:1022	a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition	967:1046	A structure-activity relationship study unveiled a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition.					
34850523	2	22	theme	medicinal	437:445	arg1	plant					447:451	a traditional Chinese medicinal plant	415:451	a traditional Chinese medicinal plant	415:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	3	23	theme	human	632:636	arg1	GalNAc-T2					638:646	human GalNAc-T2	632:646	human GalNAc-T2	632:646	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	5	24	theme	novel	969:973	arg1	pharmacophore					1010:1022	a novel quinic acid-caffeic acid conjugate pharmacophore	967:1022	a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition	967:1046	A structure-activity relationship study unveiled a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition.					
34850523	5	25	theme	acid	995:998	arg1	pharmacophore					1010:1022	a novel quinic acid-caffeic acid conjugate pharmacophore	967:1022	a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition	967:1046	A structure-activity relationship study unveiled a novel quinic acid-caffeic acid conjugate pharmacophore that directs inhibition.					
34850523	1	26	theme	initiating	211:220	arg1	enzyme					222:227	the initiating enzyme	207:227	the initiating enzyme for mucin-type O-glycosylation	207:258	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	1	26	theme	initiating	211:220	arg1	tool					131:134	a bioactive inhibitor tool	109:134	a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts)	109:204	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	2	27	theme	Chinese	429:435	arg1	plant					447:451	a traditional Chinese medicinal plant	415:451	a traditional Chinese medicinal plant	415:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	3	28	theme	O-linked	667:674	arg1	type					725:728	the other type	715:728	the other type of glycosyltransferase	715:751	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	3	28	theme	O-linked	667:674	arg1	OGT					709:711	OGT	709:711	OGT	709:711	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	3	28	theme	O-linked	667:674	arg1	transferase					696:706	O-linked N-acetylglucosamine transferase	667:706	O-linked N-acetylglucosamine transferase (OGT)	667:712	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	1	29	theme	bioactive	111:119	arg1	enzyme					222:227	the initiating enzyme	207:227	the initiating enzyme for mucin-type O-glycosylation	207:258	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	1	29	theme	bioactive	111:119	arg1	tool					131:134	a bioactive inhibitor tool	109:134	a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts)	109:204	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	2	30	theme	T2-specific	542:552	arg1	sensor					554:559	a cell-based T2-specific sensor	529:559	a cell-based T2-specific sensor	529:559	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	3	31	theme	N-acetylglucosamine	676:694	arg1	type					725:728	the other type	715:728	the other type of glycosyltransferase	715:751	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	3	31	theme	N-acetylglucosamine	676:694	arg1	OGT					709:711	OGT	709:711	OGT	709:711	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	3	31	theme	N-acetylglucosamine	676:694	arg1	transferase					696:706	O-linked N-acetylglucosamine transferase	667:706	O-linked N-acetylglucosamine transferase (OGT)	667:712	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	2	32	theme	cell-based	531:540	arg1	sensor					554:559	a cell-based T2-specific sensor	529:559	a cell-based T2-specific sensor	529:559	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	4	33	theme	elongated	838:846	arg1	O-glycans					848:856	elongated O-glycans	838:856	elongated O-glycans	838:856	Importantly, they are not cytotoxic and retain inhibitory activity in cells lacking elongated O-glycans, which are eliminated by the CRISPR/Cas9 gene editing tool.					
34850523	1	34	theme	mucin-type	233:242	arg1	O-glycosylation					244:258	mucin-type O-glycosylation	233:258	mucin-type O-glycosylation	233:258	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	2	35	theme	active	457:462	arg1	array					321:325	an array	318:325	an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant	318:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	35	theme	active	457:462	arg1	inhibitors					464:473	active inhibitors	457:473	active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay	457:585	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	1	36	theme	inhibitor	121:129	arg1	enzyme					222:227	the initiating enzyme	207:227	the initiating enzyme for mucin-type O-glycosylation	207:258	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	1	36	theme	inhibitor	121:129	arg1	tool					131:134	a bioactive inhibitor tool	109:134	a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts)	109:204	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	2	37	from	Tussilago	396:404	arg1	1-4					386:388	1-4	386:388	1-4	386:388	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	37	from	Tussilago	396:404	arg1	glycerates					374:383	four new glycerates	365:383	four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant	365:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	1	38	theme	tool	131:134	arg1	discovery					96:104	The discovery	92:104	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation,	92:259	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	2	39	theme	enzyme	574:579	arg1	assay					581:585	purified enzyme assay	565:585	purified enzyme assay	565:585	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	40	theme	present	289:295	arg1	study					297:301	the present study	285:301	the present study	285:301	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	41	theme	derivatives	342:352	arg1	array					321:325	an array	318:325	an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant	318:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	41	theme	derivatives	342:352	arg1	inhibitors					464:473	active inhibitors	457:473	active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay	457:585	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	42	theme	purified	565:572	arg1	assay					581:585	purified enzyme assay	565:585	purified enzyme assay	565:585	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	43	theme	acid	337:340	arg1	glycerates					374:383	four new glycerates	365:383	four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant	365:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	43	theme	acid	337:340	arg1	derivatives					342:352	quinic acid derivatives	330:352	quinic acid derivatives	330:352	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	6	44	theme	product	1076:1082	arg1	basis					1112:1116	a basis	1110:1116	a basis for developing an inhibitor tool for GalNAc-T2	1110:1163	Overall, these new natural product inhibitors could serve as a basis for developing an inhibitor tool for GalNAc-T2.					
34850523	6	44	theme	product	1076:1082	arg1	inhibitors					1084:1093	these new natural product inhibitors	1058:1093	these new natural product inhibitors	1058:1093	Overall, these new natural product inhibitors could serve as a basis for developing an inhibitor tool for GalNAc-T2.					
34850523	2	45	theme	combined	496:503	arg1	approach					515:522	a combined screening approach	494:522	a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay	494:585	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	46	dep	Tussilago	396:404	arg1	plant					447:451	a traditional Chinese medicinal plant	415:451	a traditional Chinese medicinal plant	415:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	46	dep	Tussilago	396:404	arg1	farfara					406:412	Tussilago farfara, a traditional Chinese medicinal plant	396:451	farfara	406:412	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	2	47	theme	traditional	417:427	arg1	plant					447:451	a traditional Chinese medicinal plant	415:451	a traditional Chinese medicinal plant	415:451	In the present study, we identified an array of quinic acid derivatives, including four new glycerates (1-4) from Tussilago farfara, a traditional Chinese medicinal plant, as active inhibitors of GalNAc-T2 using a combined screening approach with a cell-based T2-specific sensor and purified enzyme assay.					
34850523	1	48	theme	polypeptide	146:156	arg1	GalNAc-Ts					195:203	GalNAc-Ts	195:203	GalNAc-Ts	195:203	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	1	48	theme	polypeptide	146:156	arg1	transferases					181:192	human polypeptide N-acetylgalactosaminyl transferases	140:192	human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts)	140:204	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	6	49	theme	natural	1068:1074	arg1	basis					1112:1116	a basis	1110:1116	a basis for developing an inhibitor tool for GalNAc-T2	1110:1163	Overall, these new natural product inhibitors could serve as a basis for developing an inhibitor tool for GalNAc-T2.					
34850523	6	49	theme	natural	1068:1074	arg1	inhibitors					1084:1093	these new natural product inhibitors	1058:1093	these new natural product inhibitors	1058:1093	Overall, these new natural product inhibitors could serve as a basis for developing an inhibitor tool for GalNAc-T2.					
34850523	0	50	theme	Polypeptide	60:70	arg1	GalNAc-Transferase					72:89	Polypeptide GalNAc-Transferase	60:89	Polypeptide GalNAc-Transferase	60:89	Novel Quinic Acid Glycerates from Tussilago farfara Inhibit Polypeptide GalNAc-Transferase.					
34850523	3	51	theme	other	719:723	arg1	type					725:728	the other type	715:728	the other type of glycosyltransferase	715:751	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	3	51	theme	other	719:723	arg1	transferase					696:706	O-linked N-acetylglucosamine transferase	667:706	O-linked N-acetylglucosamine transferase (OGT)	667:712	These inhibitors dose-dependently inhibited human GalNAc-T2 but did not affect O-linked N-acetylglucosamine transferase (OGT), the other type of glycosyltransferase.					
34850523	0	52	theme	Acid	13:16	arg1	Glycerates					18:27	Novel Quinic Acid Glycerates	0:27	Novel Quinic Acid Glycerates from Tussilago farfara	0:50	Novel Quinic Acid Glycerates from Tussilago farfara Inhibit Polypeptide GalNAc-Transferase.					
34850523	1	53	theme	N-acetylgalactosaminyl	158:179	arg1	GalNAc-Ts					195:203	GalNAc-Ts	195:203	GalNAc-Ts	195:203	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	1	53	theme	N-acetylgalactosaminyl	158:179	arg1	transferases					181:192	human polypeptide N-acetylgalactosaminyl transferases	140:192	human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts)	140:204	The discovery of a bioactive inhibitor tool for human polypeptide N-acetylgalactosaminyl transferases (GalNAc-Ts), the initiating enzyme for mucin-type O-glycosylation, remains challenging.					
34850523	6	54	theme	new	1064:1066	arg1	basis					1112:1116	a basis	1110:1116	a basis for developing an inhibitor tool for GalNAc-T2	1110:1163	Overall, these new natural product inhibitors could serve as a basis for developing an inhibitor tool for GalNAc-T2.					
34850523	6	54	theme	new	1064:1066	arg1	inhibitors					1084:1093	these new natural product inhibitors	1058:1093	these new natural product inhibitors	1058:1093	Overall, these new natural product inhibitors could serve as a basis for developing an inhibitor tool for GalNAc-T2.					
32088229	4	0	theme	%	683:683	arg1	concentration					709:721	yeast extract concentration	695:721	yeast extract concentration	695:721	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	0	theme	%	683:683	arg1	concentration					663:675	sucrose concentration	655:675	sucrose concentration of 5.5% (w/v)	655:689	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	0	theme	%	683:683	arg1	pH					644:645	pH	644:645	pH of 6.5	644:652	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	0	theme	%	683:683	arg1	conditions					632:641	optimum conditions	624:641	optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v))	624:736	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	1	theme	pullulan	509:516	arg1	optimization					529:540	The pullulan production optimization	505:540	The pullulan production optimization by central composite design (CCD)	505:574	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	6	2	theme	wide	1028:1031	arg1	range					1033:1037	a wide range	1026:1037	a wide range of concentrations	1026:1055	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	6	3	theme	pullulan	939:946	arg1	Td					919:920	Td	919:920	Td	919:920	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	6	3	theme	pullulan	939:946	arg1	temperature					906:916	The decomposition temperature	888:916	The decomposition temperature (Td) of the produced pullulan	888:946	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	0	4	theme	pullulans	101:109	arg1	strain					77:82	a newly identified strain	58:82	a newly identified strain of Aureobasidium pullulans	58:109	Optimization and characterization of pullulan produced by a newly identified strain of Aureobasidium pullulans.					
32088229	4	5	theme	%	729:729	arg1	concentration					709:721	yeast extract concentration	695:721	yeast extract concentration	695:721	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	5	theme	%	729:729	arg1	concentration					663:675	sucrose concentration	655:675	sucrose concentration of 5.5% (w/v)	655:689	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	5	theme	%	729:729	arg1	pH					644:645	pH	644:645	pH of 6.5	644:652	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	5	theme	%	729:729	arg1	conditions					632:641	optimum conditions	624:641	optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v))	624:736	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	6	6	theme	produced	930:937	arg1	pullulan					939:946	the produced pullulan	926:946	the produced pullulan	926:946	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	0	7	theme	Aureobasidium	87:99	arg1	pullulans					101:109	Aureobasidium pullulans	87:109	Aureobasidium pullulans	87:109	Optimization and characterization of pullulan produced by a newly identified strain of Aureobasidium pullulans.					
32088229	6	8	theme	Newtonian	999:1007	arg1	behavior					1014:1021	a Newtonian flow behavior	997:1021	a Newtonian flow behavior	997:1021	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	4	9	theme	6.5	650:652	arg1	concentration					709:721	yeast extract concentration	695:721	yeast extract concentration	695:721	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	9	theme	6.5	650:652	arg1	concentration					663:675	sucrose concentration	655:675	sucrose concentration of 5.5% (w/v)	655:689	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	9	theme	6.5	650:652	arg1	pH					644:645	pH	644:645	pH of 6.5	644:652	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	9	theme	6.5	650:652	arg1	conditions					632:641	optimum conditions	624:641	optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v))	624:736	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	6	10	theme	concentrations	1042:1055	arg1	range					1033:1037	a wide range	1026:1037	a wide range of concentrations	1026:1055	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	1	11	theme	Aureobasidium	156:168	arg1	pullulans					170:178	Aureobasidium pullulans	156:178	Aureobasidium pullulans	156:178	In this study, a new strain suspected to be Aureobasidium pullulans was isolated from trees leaves.					
32088229	3	12	theme	monosaccharide	372:385	arg1	composition					387:397	monosaccharide composition	372:397	monosaccharide composition	372:397	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	6	13	contain	had	993:995	arg1	pullulan					984:991	the resulting pullulan	970:991	the resulting pullulan	970:991	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	6	13	contain	had	993:995	arg2	behavior					1014:1021	a Newtonian flow behavior	997:1021	a Newtonian flow behavior	997:1021	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	4	14	theme	extract	701:707	arg1	concentration					709:721	yeast extract concentration	695:721	yeast extract concentration	695:721	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	14	theme	extract	701:707	arg1	conditions					632:641	optimum conditions	624:641	optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v))	624:736	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	15	theme	sucrose	655:661	arg1	concentration					663:675	sucrose concentration	655:675	sucrose concentration of 5.5% (w/v)	655:689	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	15	theme	sucrose	655:661	arg1	conditions					632:641	optimum conditions	624:641	optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v))	624:736	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	3	16	theme	composition	387:397	arg1	results					339:345	the results	335:345	the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD	335:416	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	3	17	from	strain	484:489	arg1	exo-polysaccharide					446:463	the obtained exo-polysaccharide	433:463	the obtained exo-polysaccharide from the mentioned strain	433:489	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	3	17	from	strain	484:489	arg1	pullulan					495:502	pullulan	495:502	pullulan	495:502	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	4	18	theme	yeast	695:699	arg1	concentration					709:721	yeast extract concentration	695:721	yeast extract concentration	695:721	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	18	theme	yeast	695:699	arg1	conditions					632:641	optimum conditions	624:641	optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v))	624:736	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	3	19	theme	XRD	414:416	arg1	results					339:345	the results	335:345	the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD	335:416	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	3	20	theme	analysis	362:369	arg1	results					339:345	the results	335:345	the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD	335:416	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	4	21	theme	composite	553:561	arg1	design					563:568	central composite design	545:568	central composite design (CCD)	545:574	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	21	theme	composite	553:561	arg1	CCD					571:573	CCD	571:573	CCD	571:573	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	5	22	contain	had	781:783	arg2	Mw					814:815	Mw	814:815	Mw	814:815	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	5	22	contain	had	781:783	arg2	weight					806:811	an average molecular weight	785:811	an average molecular weight (Mw) of 2.07 × 105 g.mol-1	785:838	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	5	22	contain	had	781:783	arg1	pullulan					772:779	The produced pullulan	759:779	The produced pullulan	759:779	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	2	23	theme	isolated	295:302	arg1	strain					304:309	the isolated strain	291:309	the isolated strain	291:309	The molecular characterization and the resulting phylogenetic tree showed that the isolated strain was A. pullulans.					
32088229	2	23	theme	isolated	295:302	arg1	pullulans					318:326	pullulans	318:326	pullulans	318:326	The molecular characterization and the resulting phylogenetic tree showed that the isolated strain was A. pullulans.					
32088229	4	24	theme	optimum	624:630	arg1	concentration					709:721	yeast extract concentration	695:721	yeast extract concentration	695:721	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	24	theme	optimum	624:630	arg1	concentration					663:675	sucrose concentration	655:675	sucrose concentration of 5.5% (w/v)	655:689	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	24	theme	optimum	624:630	arg1	pH					644:645	pH	644:645	pH of 6.5	644:652	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	24	theme	optimum	624:630	arg1	conditions					632:641	optimum conditions	624:641	optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v))	624:736	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	5	25	theme	permeation	853:862	arg1	chromatography					864:877	gel permeation chromatography	849:877	gel permeation chromatography results	849:885	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	6	26	theme	resulting	974:982	arg1	pullulan					984:991	the resulting pullulan	970:991	the resulting pullulan	970:991	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	5	27	theme	average	788:794	arg1	Mw					814:815	Mw	814:815	Mw	814:815	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	5	27	theme	average	788:794	arg1	weight					806:811	an average molecular weight	785:811	an average molecular weight (Mw) of 2.07 × 105 g.mol-1	785:838	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	0	28	theme	pullulan	37:44	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Optimization and characterization of pullulan produced by a newly identified strain of Aureobasidium pullulans.					
32088229	0	28	theme	pullulan	37:44	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization and characterization of pullulan produced by a newly identified strain of Aureobasidium pullulans.					
32088229	3	29	theme	NMR	406:408	arg1	results					339:345	the results	335:345	the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD	335:416	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	2	30	theme	molecular	216:224	arg1	characterization					226:241	The molecular characterization	212:241	The molecular characterization	212:241	The molecular characterization and the resulting phylogenetic tree showed that the isolated strain was A. pullulans.					
32088229	3	31	theme	obtained	437:444	arg1	exo-polysaccharide					446:463	the obtained exo-polysaccharide	433:463	the obtained exo-polysaccharide from the mentioned strain	433:489	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	3	31	theme	obtained	437:444	arg1	pullulan					495:502	pullulan	495:502	pullulan	495:502	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	4	32	theme	production	518:527	arg1	optimization					529:540	The pullulan production optimization	505:540	The pullulan production optimization by central composite design (CCD)	505:574	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	33	dep	conditions	632:641	arg1	concentration					709:721	yeast extract concentration	695:721	yeast extract concentration	695:721	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	33	dep	conditions	632:641	arg1	concentration					663:675	sucrose concentration	655:675	sucrose concentration of 5.5% (w/v)	655:689	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	33	dep	conditions	632:641	arg1	pH					644:645	pH	644:645	pH of 6.5	644:652	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	33	dep	conditions	632:641	arg1	conditions					632:641	optimum conditions	624:641	optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v))	624:736	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	5	34	theme	gel	849:851	arg1	chromatography					864:877	gel permeation chromatography	849:877	gel permeation chromatography results	849:885	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	3	35	theme	FTIR	400:403	arg1	results					339:345	the results	335:345	the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD	335:416	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	4	36	theme	central	545:551	arg1	design					563:568	central composite design	545:568	central composite design (CCD)	545:574	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	36	theme	central	545:551	arg1	CCD					571:573	CCD	571:573	CCD	571:573	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	5	37	theme	produced	763:770	arg1	pullulan					772:779	The produced pullulan	759:779	The produced pullulan	759:779	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	1	38	dep	trees	198:202	arg1	leaves					204:209	leaves	204:209	leaves	204:209	In this study, a new strain suspected to be Aureobasidium pullulans was isolated from trees leaves.					
32088229	1	39	attach	isolated	184:191	arg2	strain					133:138	a new strain	127:138	a new strain suspected to be Aureobasidium pullulans	127:178	In this study, a new strain suspected to be Aureobasidium pullulans was isolated from trees leaves.					
32088229	1	39	attach	isolated	184:191	arg1	trees					198:202	trees leaves	198:209	trees leaves	198:209	In this study, a new strain suspected to be Aureobasidium pullulans was isolated from trees leaves.					
32088229	2	40	theme	phylogenetic	261:272	arg1	tree					274:277	the resulting phylogenetic tree	247:277	the resulting phylogenetic tree	247:277	The molecular characterization and the resulting phylogenetic tree showed that the isolated strain was A. pullulans.					
32088229	5	41	theme	chromatography	864:877	arg1	results					879:885	gel permeation chromatography results	849:885	gel permeation chromatography results	849:885	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	2	42	theme	resulting	251:259	arg1	tree					274:277	the resulting phylogenetic tree	247:277	the resulting phylogenetic tree	247:277	The molecular characterization and the resulting phylogenetic tree showed that the isolated strain was A. pullulans.					
32088229	3	43	theme	mentioned	474:482	arg1	strain					484:489	the mentioned strain	470:489	the mentioned strain	470:489	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32088229	4	44	theme	maximum	595:601	arg1	yield					603:607	the maximum yield	591:607	the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v))	591:736	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	4	44	theme	maximum	595:601	arg1	51.4 ± 0.50 g/L					742:756	51.4 ± 0.50 g/L	742:756	51.4 ± 0.50 g/L	742:756	The pullulan production optimization by central composite design (CCD) indicated that the maximum yield obtained under optimum conditions (pH of 6.5, sucrose concentration of 5.5% (w/v) and yeast extract concentration of 0.1% (w/v)) was 51.4 ± 0.50 g/L.					
32088229	0	45	theme	identified	66:75	arg1	strain					77:82	a newly identified strain	58:82	a newly identified strain of Aureobasidium pullulans	58:109	Optimization and characterization of pullulan produced by a newly identified strain of Aureobasidium pullulans.					
32088229	5	46	theme	molecular	796:804	arg1	Mw					814:815	Mw	814:815	Mw	814:815	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	5	46	theme	molecular	796:804	arg1	weight					806:811	an average molecular weight	785:811	an average molecular weight (Mw) of 2.07 × 105 g.mol-1	785:838	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	1	47	theme	new	129:131	arg1	strain					133:138	a new strain	127:138	a new strain suspected to be Aureobasidium pullulans	127:178	In this study, a new strain suspected to be Aureobasidium pullulans was isolated from trees leaves.					
32088229	6	48	theme	flow	1009:1012	arg1	behavior					1014:1021	a Newtonian flow behavior	997:1021	a Newtonian flow behavior	997:1021	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	5	49	theme	2.07 × 105 g.mol-1	821:838	arg1	Mw					814:815	Mw	814:815	Mw	814:815	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	5	49	theme	2.07 × 105 g.mol-1	821:838	arg1	weight					806:811	an average molecular weight	785:811	an average molecular weight (Mw) of 2.07 × 105 g.mol-1	785:838	The produced pullulan had an average molecular weight (Mw) of 2.07 × 105 g.mol-1 based on gel permeation chromatography results.					
32088229	6	50	theme	decomposition	892:904	arg1	Td					919:920	Td	919:920	Td	919:920	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	6	50	theme	decomposition	892:904	arg1	temperature					906:916	The decomposition temperature	888:916	The decomposition temperature (Td) of the produced pullulan	888:946	The decomposition temperature (Td) of the produced pullulan was ~300 °C and also, the resulting pullulan had a Newtonian flow behavior in a wide range of concentrations.					
32088229	3	51	theme	methylation	350:360	arg1	analysis					362:369	methylation analysis	350:369	methylation analysis	350:369	Also, the results of methylation analysis, monosaccharide composition, FTIR, NMR and XRD confirmed that the obtained exo-polysaccharide from the mentioned strain was pullulan.					
32737949	6	0	theme	scurfy	1007:1012	arg1	mice					1014:1017	wild-type and scurfy mice	993:1017	wild-type and scurfy mice	993:1017	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	10	1	from	pattern	1809:1815	arg1	cells					1864:1868	immune cells	1857:1868	immune cells	1857:1868	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	7	2	theme	various	1232:1238	arg1	receptors					1254:1262	various C-type lectin receptors	1232:1262	various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice	1232:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	10	3	theme	immune	1857:1862	arg1	cells					1864:1868	immune cells	1857:1868	immune cells	1857:1868	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	7	4	used	used	1194:1197	arg2	ChipCytometry					1175:1187	ChipCytometry	1175:1187	ChipCytometry	1175:1187	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	7	4	used	used	1194:1197	arg2	cytometry					1161:1169	flow cytometry	1156:1169	flow cytometry	1156:1169	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	6	5	theme	wild-type	993:1001	arg1	mice					1014:1017	wild-type and scurfy mice	993:1017	wild-type and scurfy mice	993:1017	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	9	6	from	levels	1657:1662	arg1	cells					1707:1711	antigen-presenting cells	1688:1711	antigen-presenting cells	1688:1711	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	9	7	theme	functional	1551:1560	arg1	cells					1575:1579	functional regulatory T cells	1551:1579	functional regulatory T cells	1551:1579	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	6	8	theme	cells	982:986	arg1	Glycosphingolipids					925:942	Glycosphingolipids	925:942	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice	925:1017	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	2	9	from	existence	360:368	arg1	cells					409:413	immune cells	402:413	immune cells	402:413	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	2	9	from	existence	360:368	arg1	proteins					433:440	extracellular proteins	419:440	extracellular proteins	419:440	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	10	10	theme	regulatory	1771:1780	arg1	cells					1784:1788	functional regulatory T cells	1760:1788	functional regulatory T cells	1760:1788	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	4	11	theme	forkhead-box-P3-deficient	730:754	arg1	mice					763:766	forkhead-box-P3-deficient scurfy mice	730:766	forkhead-box-P3-deficient scurfy mice	730:766	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice differs from those on wild-type immune cells.					
32737949	7	12	theme	access	1202:1207	arg1	patterns					1220:1227	access expression patterns	1202:1227	access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice	1202:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	7	13	theme	various	1297:1303	arg1	organs					1305:1310	various organs	1297:1310	various organs of both wild-type and scurfy mice	1297:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	9	14	theme	T	1573:1573	arg1	cells					1575:1579	functional regulatory T cells	1551:1579	functional regulatory T cells	1551:1579	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	6	15	theme	splenic	964:970	arg1	cells					982:986	splenic dendritic cells	964:986	splenic dendritic cells	964:986	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	4	16	theme	mice	763:766	arg1	cells					721:725	immune cells	714:725	immune cells of forkhead-box-P3-deficient scurfy mice	714:766	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice differs from those on wild-type immune cells.					
32737949	11	17	theme	homeostatic	1948:1958	arg1	perturbances					1960:1971	genetical and homeostatic perturbances	1934:1971	genetical and homeostatic perturbances such as those resulting from a diseased condition	1934:2021	Thus, glycosphingolipids could serve as biomarkers for mapping genetical and homeostatic perturbances such as those resulting from a diseased condition.					
32737949	11	17	theme	homeostatic	1948:1958	arg1	those					1981:1985	those	1981:1985	those	1981:1985	Thus, glycosphingolipids could serve as biomarkers for mapping genetical and homeostatic perturbances such as those resulting from a diseased condition.					
32737949	2	18	theme	glycan	329:334	arg1	hypothesis					336:345	the "altered glycan hypothesis"	316:346	the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns	316:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	6	19	theme	gel	1063:1065	arg1	electrophoresis					1067:1081	multiplexed capillary gel electrophoresis	1041:1081	multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	1041:1140	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	9	20	theme	regulatory	1562:1571	arg1	cells					1575:1579	functional regulatory T cells	1551:1579	functional regulatory T cells	1551:1579	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	8	21	theme	differential	1373:1384	arg1	expression					1386:1395	differential expression	1373:1395	differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild-type and scurfy mice	1373:1503	RESULTS We, hereby report differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild-type and scurfy mice.					
32737949	11	22	theme	genetical	1934:1942	arg1	perturbances					1960:1971	genetical and homeostatic perturbances	1934:1971	genetical and homeostatic perturbances such as those resulting from a diseased condition	1934:2021	Thus, glycosphingolipids could serve as biomarkers for mapping genetical and homeostatic perturbances such as those resulting from a diseased condition.					
32737949	11	22	theme	genetical	1934:1942	arg1	those					1981:1985	those	1981:1985	those	1981:1985	Thus, glycosphingolipids could serve as biomarkers for mapping genetical and homeostatic perturbances such as those resulting from a diseased condition.					
32737949	0	23	theme	regulatory	124:133	arg1	cells					137:141	functional regulatory T cells	113:141	functional regulatory T cells	113:141	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	1	24	theme	protein	201:207	arg1	modification					227:238	protein posttranslational modification	201:238	protein posttranslational modification	201:238	BACKGROUND Glycosylation is a common and complex type of protein posttranslational modification.					
32737949	6	25	theme	multiplexed	1041:1051	arg1	electrophoresis					1067:1081	multiplexed capillary gel electrophoresis	1041:1081	multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	1041:1140	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	0	26	theme	cells	137:141	arg1	absence					102:108	absence	102:108	absence of functional regulatory T cells	102:141	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	7	27	theme	antigen-presenting	1267:1284	arg1	cells					1286:1290	antigen-presenting cells	1267:1290	antigen-presenting cells from various organs of both wild-type and scurfy mice	1267:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	1	28	theme	modification	227:238	arg1	Glycosylation					155:167	BACKGROUND Glycosylation	144:167	BACKGROUND Glycosylation	144:167	BACKGROUND Glycosylation is a common and complex type of protein posttranslational modification.					
32737949	1	28	theme	modification	227:238	arg1	type					193:196	a common and complex type	172:196	a common and complex type of protein posttranslational modification	172:238	BACKGROUND Glycosylation is a common and complex type of protein posttranslational modification.					
32737949	4	29	theme	immune	800:805	arg1	cells					807:811	wild-type immune cells	790:811	wild-type immune cells	790:811	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice differs from those on wild-type immune cells.					
32737949	6	30	from	Glycosphingolipids	925:942	arg1	mice					1014:1017	wild-type and scurfy mice	993:1017	wild-type and scurfy mice	993:1017	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	9	31	from	cells	1707:1711	arg1	levels					1657:1662	levels	1657:1662	levels of CLEC12A and CD206 on antigen-presenting cells	1657:1711	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	10	32	from	quantities	1821:1830	arg1	cells					1864:1868	immune cells	1857:1868	immune cells	1857:1868	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	2	33	theme	autoimmune	285:294	arg1	diseases					296:303	autoimmune diseases	285:303	autoimmune diseases	285:303	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	8	34	theme	inflammatory	1439:1450	arg1	conditions					1452:1461	inflammatory conditions	1439:1461	inflammatory conditions as reflected in wild-type and scurfy mice	1439:1503	RESULTS We, hereby report differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild-type and scurfy mice.					
32737949	4	35	from	HYPOTHESIS	673:682	arg1	cells					721:725	immune cells	714:725	immune cells of forkhead-box-P3-deficient scurfy mice	714:766	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice differs from those on wild-type immune cells.					
32737949	2	36	theme	immunoglobulins	266:280	arg1	glycosylation					249:261	Altered glycosylation	241:261	Altered glycosylation of immunoglobulins in autoimmune diseases	241:303	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	2	37	theme	patterns	543:550	arg1	abundances					482:491	site-specific relative abundances	459:491	site-specific relative abundances of individual glycan structures and glycosylation patterns	459:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	0	38	from	patterns	24:31	arg1	cells					93:97	immune cells	86:97	immune cells in absence of functional regulatory T cells	86:141	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	0	39	theme	immune	86:91	arg1	cells					93:97	immune cells	86:97	immune cells in absence of functional regulatory T cells	86:141	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	6	40	theme	laser-induced	1094:1106	arg1	xCGE-LIF					1132:1139	xCGE-LIF	1132:1139	xCGE-LIF	1132:1139	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	6	40	theme	laser-induced	1094:1106	arg1	detection					1121:1129	laser-induced fluorescence detection	1094:1129	laser-induced fluorescence detection (xCGE-LIF)	1094:1140	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	5	41	theme	T	822:822	arg1	cells					824:828	METHODS T cells	814:828	METHODS T cells	814:828	METHODS T cells and dendritic cells were isolated from spleens of either wild-type or age-matched scurfy mice.					
32737949	9	42	theme	CLEC12A	1667:1673	arg1	levels					1657:1662	levels	1657:1662	levels of CLEC12A and CD206 on antigen-presenting cells	1657:1711	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	7	43	from	cells	1286:1290	arg1	patterns					1220:1227	access expression patterns	1202:1227	access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice	1202:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	10	44	theme	cells	1784:1788	arg1	absence					1749:1755	the absence	1745:1755	the absence of functional regulatory T cells	1745:1788	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	9	45	theme	CD206	1679:1683	arg1	levels					1657:1662	levels	1657:1662	levels of CLEC12A and CD206 on antigen-presenting cells	1657:1711	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	7	46	theme	mice	1341:1344	arg1	organs					1305:1310	various organs	1297:1310	various organs of both wild-type and scurfy mice	1297:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	2	47	theme	immune	402:407	arg1	cells					409:413	immune cells	402:413	immune cells	402:413	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	0	48	theme	Differential	0:11	arg1	patterns					24:31	Differential expression patterns	0:31	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.	0:142	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	9	49	theme	antigen-presenting	1688:1705	arg1	cells					1707:1711	antigen-presenting cells	1688:1711	antigen-presenting cells	1688:1711	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	2	50	theme	relative	473:480	arg1	abundances					482:491	site-specific relative abundances	459:491	site-specific relative abundances of individual glycan structures and glycosylation patterns	459:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	9	51	from	reduction	1644:1652	arg1	levels					1657:1662	levels	1657:1662	levels of CLEC12A and CD206 on antigen-presenting cells	1657:1711	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	5	52	theme	scurfy	912:917	arg1	mice					919:922	either wild-type or age-matched scurfy mice	880:922	either wild-type or age-matched scurfy mice	880:922	METHODS T cells and dendritic cells were isolated from spleens of either wild-type or age-matched scurfy mice.					
32737949	8	53	dep	RESULTS	1347:1353	arg1	We					1355:1356	We	1355:1356	We	1355:1356	RESULTS We, hereby report differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild-type and scurfy mice.					
32737949	2	54	from	diseases	296:303	arg1	glycosylation					249:261	Altered glycosylation	241:261	Altered glycosylation of immunoglobulins in autoimmune diseases	241:303	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	10	55	theme	expression	1798:1807	arg1	pattern					1809:1815	expression pattern	1798:1815	expression pattern	1798:1815	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	0	56	theme	glycosphingolipids	36:53	arg1	patterns					24:31	Differential expression patterns	0:31	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.	0:142	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	6	57	theme	T	952:952	arg1	cells					954:958	CD4+ T cells	947:958	CD4+ T cells	947:958	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	8	58	dep	conditions	1452:1461	arg1	reflected					1466:1474	reflected	1466:1474	reflected in wild-type and scurfy mice	1466:1503	RESULTS We, hereby report differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild-type and scurfy mice.					
32737949	2	59	theme	glycan	507:512	arg1	structures					514:523	individual glycan structures	496:523	individual glycan structures	496:523	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	7	60	from	patterns	1220:1227	arg1	cells					1286:1290	antigen-presenting cells	1267:1290	antigen-presenting cells from various organs of both wild-type and scurfy mice	1267:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	0	61	theme	receptors	73:81	arg1	patterns					24:31	Differential expression patterns	0:31	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.	0:142	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	11	62	theme	diseased	2004:2011	arg1	condition					2013:2021	a diseased condition	2002:2021	a diseased condition	2002:2021	Thus, glycosphingolipids could serve as biomarkers for mapping genetical and homeostatic perturbances such as those resulting from a diseased condition.					
32737949	0	63	theme	C-type	59:64	arg1	receptors					73:81	C-type lectin receptors	59:81	C-type lectin receptors	59:81	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	10	64	dep	CONCLUSION	1714:1723	arg1	show					1735:1738	show	1735:1738	show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells	1735:1868	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	2	65	from	proteins	433:440	arg1	existence					360:368	existence	360:368	existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns	360:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	2	66	theme	signature	389:397	arg1	existence					360:368	existence	360:368	existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns	360:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	7	67	theme	C-type	1240:1245	arg1	receptors					1254:1262	various C-type lectin receptors	1232:1262	various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice	1232:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	6	68	from	mice	1014:1017	arg1	Glycosphingolipids					925:942	Glycosphingolipids	925:942	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice	925:1017	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	6	68	from	mice	1014:1017	arg1	cells					982:986	splenic dendritic cells	964:986	splenic dendritic cells	964:986	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	6	68	from	mice	1014:1017	arg1	cells					954:958	CD4+ T cells	947:958	CD4+ T cells	947:958	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	2	69	theme	unique	375:380	arg1	signature					389:397	a unique glycan signature	373:397	a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns	373:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	7	70	theme	expression	1209:1218	arg1	patterns					1220:1227	access expression patterns	1202:1227	access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice	1202:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	8	71	theme	glycosphingolipids	1400:1417	arg1	expression					1386:1395	differential expression	1373:1395	differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild-type and scurfy mice	1373:1503	RESULTS We, hereby report differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild-type and scurfy mice.					
32737949	9	72	theme	cells	1575:1579	arg1	absence					1540:1546	the absence	1536:1546	the absence of functional regulatory T cells	1536:1579	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	1	73	theme	common	174:179	arg1	Glycosylation					155:167	BACKGROUND Glycosylation	144:167	BACKGROUND Glycosylation	144:167	BACKGROUND Glycosylation is a common and complex type of protein posttranslational modification.					
32737949	1	73	theme	common	174:179	arg1	type					193:196	a common and complex type	172:196	a common and complex type of protein posttranslational modification	172:238	BACKGROUND Glycosylation is a common and complex type of protein posttranslational modification.					
32737949	7	74	theme	lectin	1247:1252	arg1	receptors					1254:1262	various C-type lectin receptors	1232:1262	various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice	1232:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	6	75	theme	dendritic	972:980	arg1	cells					982:986	splenic dendritic cells	964:986	splenic dendritic cells	964:986	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	7	76	theme	scurfy	1334:1339	arg1	mice					1341:1344	both wild-type and scurfy mice	1315:1344	both wild-type and scurfy mice	1315:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	1	77	theme	complex	185:191	arg1	Glycosylation					155:167	BACKGROUND Glycosylation	144:167	BACKGROUND Glycosylation	144:167	BACKGROUND Glycosylation is a common and complex type of protein posttranslational modification.					
32737949	1	77	theme	complex	185:191	arg1	type					193:196	a common and complex type	172:196	a common and complex type of protein posttranslational modification	172:238	BACKGROUND Glycosylation is a common and complex type of protein posttranslational modification.					
32737949	2	78	from	signature	389:397	arg1	cells					409:413	immune cells	402:413	immune cells	402:413	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	2	78	from	signature	389:397	arg1	proteins					433:440	extracellular proteins	419:440	extracellular proteins	419:440	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	4	79	theme	scurfy	756:761	arg1	mice					763:766	forkhead-box-P3-deficient scurfy mice	730:766	forkhead-box-P3-deficient scurfy mice	730:766	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice differs from those on wild-type immune cells.					
32737949	0	80	theme	T	135:135	arg1	cells					137:141	functional regulatory T cells	113:141	functional regulatory T cells	113:141	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	2	81	theme	altered	321:327	arg1	hypothesis					336:345	the "altered glycan hypothesis"	316:346	the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns	316:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	5	82	theme	mice	919:922	arg1	spleens					869:875	spleens	869:875	spleens of either wild-type or age-matched scurfy mice	869:922	METHODS T cells and dendritic cells were isolated from spleens of either wild-type or age-matched scurfy mice.					
32737949	6	83	theme	capillary	1053:1061	arg1	electrophoresis					1067:1081	multiplexed capillary gel electrophoresis	1041:1081	multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF)	1041:1140	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	0	84	from	cells	93:97	arg1	absence					102:108	absence	102:108	absence of functional regulatory T cells	102:141	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	2	85	gly	glycosylation	249:261	arg1	immunoglobulins					266:280	immunoglobulins	266:280	immunoglobulins in autoimmune diseases	266:303	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	7	86	theme	flow	1156:1159	arg1	cytometry					1161:1169	flow cytometry	1156:1169	flow cytometry	1156:1169	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	3	87	theme	health	649:654	arg1	states					639:644	states	639:644	states of health or inflammation	639:670	However, it is not clear how glycosylation on leukocyte subpopulations differ between states of health or inflammation.					
32737949	1	88	theme	posttranslational	209:225	arg1	modification					227:238	protein posttranslational modification	201:238	protein posttranslational modification	201:238	BACKGROUND Glycosylation is a common and complex type of protein posttranslational modification.					
32737949	4	89	theme	immune	714:719	arg1	cells					721:725	immune cells	714:725	immune cells of forkhead-box-P3-deficient scurfy mice	714:766	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice differs from those on wild-type immune cells.					
32737949	2	90	theme	Altered	241:247	arg1	glycosylation					249:261	Altered glycosylation	241:261	Altered glycosylation of immunoglobulins in autoimmune diseases	241:303	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	9	91	theme	CLEC-7A	1620:1626	arg1	expression					1606:1615	elevated expression	1597:1615	elevated expression of CLEC-7A and CD205	1597:1636	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	4	92	theme	wild-type	790:798	arg1	cells					807:811	wild-type immune cells	790:811	wild-type immune cells	790:811	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice differs from those on wild-type immune cells.					
32737949	4	93	dep	HYPOTHESIS	673:682	arg1	patterns					702:709	Glycosphingolipid patterns	684:709	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice	673:766	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice differs from those on wild-type immune cells.					
32737949	9	94	theme	CD205	1632:1636	arg1	expression					1606:1615	elevated expression	1597:1615	elevated expression of CLEC-7A and CD205	1597:1636	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	3	95	theme	leukocyte	599:607	arg1	subpopulations					609:622	leukocyte subpopulations	599:622	leukocyte subpopulations	599:622	However, it is not clear how glycosylation on leukocyte subpopulations differ between states of health or inflammation.					
32737949	5	96	theme	dendritic	834:842	arg1	cells					844:848	dendritic cells	834:848	dendritic cells	834:848	METHODS T cells and dendritic cells were isolated from spleens of either wild-type or age-matched scurfy mice.					
32737949	3	97	theme	inflammation	659:670	arg1	states					639:644	states	639:644	states of health or inflammation	639:670	However, it is not clear how glycosylation on leukocyte subpopulations differ between states of health or inflammation.					
32737949	8	98	from	expression	1386:1395	arg1	health					1422:1427	health	1422:1427	health	1422:1427	RESULTS We, hereby report differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild-type and scurfy mice.					
32737949	1	99	theme	BACKGROUND	144:153	arg1	Glycosylation					155:167	BACKGROUND Glycosylation	144:167	BACKGROUND Glycosylation	144:167	BACKGROUND Glycosylation is a common and complex type of protein posttranslational modification.					
32737949	1	99	theme	BACKGROUND	144:153	arg1	type					193:196	a common and complex type	172:196	a common and complex type of protein posttranslational modification	172:238	BACKGROUND Glycosylation is a common and complex type of protein posttranslational modification.					
32737949	3	100	gly	glycosylation	582:594	arg1	subpopulations					609:622	leukocyte subpopulations	599:622	leukocyte subpopulations	599:622	However, it is not clear how glycosylation on leukocyte subpopulations differ between states of health or inflammation.					
32737949	7	101	theme	wild-type	1320:1328	arg1	mice					1341:1344	both wild-type and scurfy mice	1315:1344	both wild-type and scurfy mice	1315:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	9	102	from	CD206	1679:1683	arg1	cells					1707:1711	antigen-presenting cells	1688:1711	antigen-presenting cells	1688:1711	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	6	103	theme	fluorescence	1108:1119	arg1	xCGE-LIF					1132:1139	xCGE-LIF	1132:1139	xCGE-LIF	1132:1139	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	6	103	theme	fluorescence	1108:1119	arg1	detection					1121:1129	laser-induced fluorescence detection	1094:1129	laser-induced fluorescence detection (xCGE-LIF)	1094:1140	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	2	104	theme	glycosylation	529:541	arg1	patterns					543:550	glycosylation patterns	529:550	glycosylation patterns	529:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	7	105	from	receptors	1254:1262	arg1	cells					1286:1290	antigen-presenting cells	1267:1290	antigen-presenting cells from various organs of both wild-type and scurfy mice	1267:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	3	106	from	glycosylation	582:594	arg1	subpopulations					609:622	leukocyte subpopulations	599:622	leukocyte subpopulations	599:622	However, it is not clear how glycosylation on leukocyte subpopulations differ between states of health or inflammation.					
32737949	7	107	from	organs	1305:1310	arg1	cells					1286:1290	antigen-presenting cells	1267:1290	antigen-presenting cells from various organs of both wild-type and scurfy mice	1267:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	5	108	theme	METHODS	814:820	arg1	cells					824:828	METHODS T cells	814:828	METHODS T cells	814:828	METHODS T cells and dendritic cells were isolated from spleens of either wild-type or age-matched scurfy mice.					
32737949	9	109	from	CLEC12A	1667:1673	arg1	cells					1707:1711	antigen-presenting cells	1688:1711	antigen-presenting cells	1688:1711	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	0	110	theme	functional	113:122	arg1	cells					137:141	functional regulatory T cells	113:141	functional regulatory T cells	113:141	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	9	111	theme	elevated	1597:1604	arg1	expression					1606:1615	elevated expression	1597:1615	elevated expression of CLEC-7A and CD205	1597:1636	Furthermore, we observed that the absence of functional regulatory T cells correlated with elevated expression of CLEC-7A and CD205 but a reduction in levels of CLEC12A and CD206 on antigen-presenting cells.					
32737949	8	112	theme	wild-type	1479:1487	arg1	mice					1500:1503	wild-type and scurfy mice	1479:1503	wild-type and scurfy mice	1479:1503	RESULTS We, hereby report differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild-type and scurfy mice.					
32737949	10	113	theme	T	1782:1782	arg1	cells					1784:1788	functional regulatory T cells	1760:1788	functional regulatory T cells	1760:1788	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	5	114	theme	wild-type	887:895	arg1	mice					919:922	either wild-type or age-matched scurfy mice	880:922	either wild-type or age-matched scurfy mice	880:922	METHODS T cells and dendritic cells were isolated from spleens of either wild-type or age-matched scurfy mice.					
32737949	2	115	from	glycosylation	249:261	arg1	diseases					296:303	autoimmune diseases	285:303	autoimmune diseases	285:303	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	8	116	theme	scurfy	1493:1498	arg1	mice					1500:1503	wild-type and scurfy mice	1479:1503	wild-type and scurfy mice	1479:1503	RESULTS We, hereby report differential expression of glycosphingolipids in health and under inflammatory conditions as reflected in wild-type and scurfy mice.					
32737949	10	117	theme	functional	1760:1769	arg1	cells					1784:1788	functional regulatory T cells	1760:1788	functional regulatory T cells	1760:1788	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	5	118	theme	age-matched	900:910	arg1	mice					919:922	either wild-type or age-matched scurfy mice	880:922	either wild-type or age-matched scurfy mice	880:922	METHODS T cells and dendritic cells were isolated from spleens of either wild-type or age-matched scurfy mice.					
32737949	2	119	from	immunoglobulins	266:280	arg1	diseases					296:303	autoimmune diseases	285:303	autoimmune diseases	285:303	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	2	120	theme	site-specific	459:471	arg1	abundances					482:491	site-specific relative abundances	459:491	site-specific relative abundances of individual glycan structures and glycosylation patterns	459:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	7	121	theme	receptors	1254:1262	arg1	patterns					1220:1227	access expression patterns	1202:1227	access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice	1202:1344	In addition, flow cytometry and ChipCytometry were used to access expression patterns of various C-type lectin receptors on antigen-presenting cells from various organs of both wild-type and scurfy mice.					
32737949	6	122	theme	cells	954:958	arg1	Glycosphingolipids					925:942	Glycosphingolipids	925:942	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice	925:1017	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	2	123	theme	structures	514:523	arg1	abundances					482:491	site-specific relative abundances	459:491	site-specific relative abundances of individual glycan structures and glycosylation patterns	459:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	2	124	theme	extracellular	419:431	arg1	proteins					433:440	extracellular proteins	419:440	extracellular proteins	419:440	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	0	125	theme	lectin	66:71	arg1	receptors					73:81	C-type lectin receptors	59:81	C-type lectin receptors	59:81	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
32737949	6	126	theme	CD4+	947:950	arg1	cells					954:958	CD4+ T cells	947:958	CD4+ T cells	947:958	Glycosphingolipids of CD4+ T cells and splenic dendritic cells from wild-type and scurfy mice were then analyzed by multiplexed capillary gel electrophoresis coupled to laser-induced fluorescence detection (xCGE-LIF).					
32737949	2	127	theme	individual	496:505	arg1	structures					514:523	individual glycan structures	496:523	individual glycan structures	496:523	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	5	128	attach	isolated	855:862	arg2	cells					844:848	dendritic cells	834:848	dendritic cells	834:848	METHODS T cells and dendritic cells were isolated from spleens of either wild-type or age-matched scurfy mice.					
32737949	5	128	attach	isolated	855:862	arg1	spleens					869:875	spleens	869:875	spleens of either wild-type or age-matched scurfy mice	869:922	METHODS T cells and dendritic cells were isolated from spleens of either wild-type or age-matched scurfy mice.					
32737949	5	128	attach	isolated	855:862	arg2	cells					824:828	METHODS T cells	814:828	METHODS T cells	814:828	METHODS T cells and dendritic cells were isolated from spleens of either wild-type or age-matched scurfy mice.					
32737949	10	129	theme	glycosphingolipids	1835:1852	arg1	quantities					1821:1830	quantities	1821:1830	quantities	1821:1830	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	10	129	theme	glycosphingolipids	1835:1852	arg1	pattern					1809:1815	expression pattern	1798:1815	expression pattern	1798:1815	CONCLUSION We hereby show that the absence of functional regulatory T cells affects expression pattern and quantities of glycosphingolipids on immune cells.					
32737949	2	130	from	cells	409:413	arg1	existence					360:368	existence	360:368	existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns	360:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	2	131	theme	glycan	382:387	arg1	signature					389:397	a unique glycan signature	373:397	a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns	373:550	Altered glycosylation of immunoglobulins in autoimmune diseases has led to the "altered glycan hypothesis" postulating existence of a unique glycan signature on immune cells and extracellular proteins characterized by site-specific relative abundances of individual glycan structures and glycosylation patterns.					
32737949	4	132	theme	Glycosphingolipid	684:700	arg1	patterns					702:709	Glycosphingolipid patterns	684:709	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice	673:766	HYPOTHESIS Glycosphingolipid patterns on immune cells of forkhead-box-P3-deficient scurfy mice differs from those on wild-type immune cells.					
32737949	0	133	theme	expression	13:22	arg1	patterns					24:31	Differential expression patterns	0:31	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.	0:142	Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells.					
33411075	13	0	with	structure	1135:1143	arg1	patterns					1170:1177	different sulfation patterns	1150:1177	different sulfation patterns	1150:1177	CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities.					
33411075	13	0	with	structure	1135:1143	arg1	densities					1183:1191	densities	1183:1191	densities	1183:1191	CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities.					
33411075	9	1	theme	mass	781:784	arg1	LC-MS-ITTOF					800:810	LC-MS-ITTOF	800:810	LC-MS-ITTOF	800:810	and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
33411075	9	1	theme	mass	781:784	arg1	spectrometry					786:797	liquid chromatography-ion trap/time-of-flight mass spectrometry	735:797	liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	735:811	and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
33411075	13	2	contain	possess	1111:1117	arg2	structure					1135:1143	a heterogeneous structure	1119:1143	a heterogeneous structure with different sulfation patterns and densities	1119:1191	CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities.					
33411075	13	2	contain	possess	1111:1117	arg1	chains					1099:1104	CS/DS chains	1093:1104	CS/DS chains	1093:1104	CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities.					
33411075	0	3	from	analysis	11:18	arg1	ranae					57:61	Oviductus ranae	47:61	Oviductus ranae	47:61	Structural analysis of glycosaminoglycans from Oviductus ranae.					
33411075	4	4	contain	have	208:211	arg2	activities					233:242	numerous biological activities	213:242	numerous biological activities	213:242	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	4	4	contain	have	208:211	arg1	extracts					177:184	extracts	177:184	extracts	177:184	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	4	4	contain	have	208:211	arg2	activities					255:264	activities	255:264	activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic	255:388	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	12	5	theme	constituent	1073:1083	arg1	chains					1085:1090	their constituent chains	1067:1090	their constituent chains	1067:1090	HS species contain highly acetylated disaccharides, and have various structures in their constituent chains.					
33411075	1	6	theme	Oviductus	64:72	arg1	O.ran					81:85	O.ran	81:85	O.ran	81:85	Oviductus ranae (O.ran.)					
33411075	1	6	theme	Oviductus	64:72	arg1	ranae					74:78	Oviductus ranae	64:78	Oviductus ranae (O.ran.)	64:87	Oviductus ranae (O.ran.)					
33411075	11	7	theme	varied	950:955	arg1	compositions					970:981	varied disaccharide compositions	950:981	varied disaccharide compositions	950:981	with varied disaccharide compositions.					
33411075	2	8	theme	traditional	127:137	arg1	medicine					160:167	a traditional animal-based Chinese medicine	125:167	a traditional animal-based Chinese medicine	125:167	has been widely used as a tonic and a traditional animal-based Chinese medicine.					
33411075	14	9	from	involvement	1260:1270	arg1	activities					1315:1324	the biological activities	1300:1324	the biological activities of O.ran	1300:1333	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of O.ran.					
33411075	14	10	theme	possible	1251:1258	arg1	involvement					1260:1270	the possible involvement	1247:1270	the possible involvement of these polysaccharides in the biological activities of O.ran	1247:1333	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of O.ran.					
33411075	11	11	theme	disaccharide	957:968	arg1	compositions					970:981	varied disaccharide compositions	950:981	varied disaccharide compositions	950:981	with varied disaccharide compositions.					
33411075	6	12	theme	structural	556:565	arg1	characteristics					567:581	possible structural characteristics	547:581	possible structural characteristics	547:581	However, their presence and possible structural characteristics within O.ran.					
33411075	0	13	from	ranae	57:61	arg1	glycosaminoglycans					23:40	glycosaminoglycans	23:40	glycosaminoglycans from Oviductus ranae	23:61	Structural analysis of glycosaminoglycans from Oviductus ranae.					
33411075	0	13	from	ranae	57:61	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of glycosaminoglycans from Oviductus ranae	0:61	Structural analysis of glycosaminoglycans from Oviductus ranae.					
33411075	12	14	contain	have	1040:1043	arg1	species					987:993	HS species	984:993	HS species	984:993	HS species contain highly acetylated disaccharides, and have various structures in their constituent chains.					
33411075	12	14	contain	have	1040:1043	arg2	structures					1053:1062	various structures	1045:1062	various structures in their constituent chains	1045:1090	HS species contain highly acetylated disaccharides, and have various structures in their constituent chains.					
33411075	10	15	theme	/dermatan	873:881	arg1	DS					892:893	DS	892:893	DS	892:893	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	15	theme	/dermatan	873:881	arg1	sulfate					883:889	/dermatan sulfate	873:889	/dermatan sulfate (DS)	873:894	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	6	16	theme	possible	547:554	arg1	characteristics					567:581	possible structural characteristics	547:581	possible structural characteristics	547:581	However, their presence and possible structural characteristics within O.ran.					
33411075	13	17	theme	different	1150:1158	arg1	patterns					1170:1177	different sulfation patterns	1150:1177	different sulfation patterns	1150:1177	CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities.					
33411075	5	18	from	mammals	459:465	arg1	ubiquitous					445:454	ubiquitous	445:454	ubiquitous	445:454	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	4	19	theme	mammalian	297:305	arg1	antiosteoperotic					353:368	antiosteoperotic	353:368	antiosteoperotic	353:368	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	4	19	theme	mammalian	297:305	arg1	glycosaminoglycans					307:324	mammalian glycosaminoglycans	297:324	mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic	297:388	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	4	19	theme	mammalian	297:305	arg1	anti-inflammatory					334:350	anti-inflammatory	334:350	anti-inflammatory	334:350	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	4	19	theme	mammalian	297:305	arg1	anti-asthmatic					375:388	anti-asthmatic	375:388	anti-asthmatic	375:388	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	13	20	theme	sulfation	1160:1168	arg1	patterns					1170:1177	different sulfation patterns	1150:1177	different sulfation patterns	1150:1177	CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities.					
33411075	14	21	theme	polysaccharides	1281:1295	arg1	involvement					1260:1270	the possible involvement	1247:1270	the possible involvement of these polysaccharides in the biological activities of O.ran	1247:1333	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of O.ran.					
33411075	0	22	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of glycosaminoglycans from Oviductus ranae	0:61	Structural analysis of glycosaminoglycans from Oviductus ranae.					
33411075	12	23	contain	contain	995:1001	arg2	disaccharides					1021:1033	highly acetylated disaccharides	1003:1033	highly acetylated disaccharides	1003:1033	HS species contain highly acetylated disaccharides, and have various structures in their constituent chains.					
33411075	12	23	contain	contain	995:1001	arg1	species					987:993	HS species	984:993	HS species	984:993	HS species contain highly acetylated disaccharides, and have various structures in their constituent chains.					
33411075	14	24	theme	O.ran	1329:1333	arg1	activities					1315:1324	the biological activities	1300:1324	the biological activities of O.ran	1300:1333	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of O.ran.					
33411075	5	25	theme	biological	496:505	arg1	activities					507:516	biological activities	496:516	biological activities	496:516	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	0	26	theme	glycosaminoglycans	23:40	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of glycosaminoglycans from Oviductus ranae	0:61	Structural analysis of glycosaminoglycans from Oviductus ranae.					
33411075	10	27	dep	sulfate	822:828	arg1	HP					844:845	HP	844:845	HP	844:845	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	27	dep	sulfate	822:828	arg1	/heparin					834:841	/heparin	834:841	Heparan sulfate (HS)/heparin (HP)	814:846	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	5	28	theme	activities	507:516	arg1	range					487:491	a wide range	480:491	a wide range of biological activities	480:516	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	10	29	located	detected	926:933	arg2	sulfate					861:867	chondroitin sulfate	849:867	chondroitin sulfate	849:867	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	29	located	detected	926:933	arg2	sulfate					883:889	/dermatan sulfate	873:889	/dermatan sulfate (DS)	873:894	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	29	located	detected	926:933	arg2	acid					911:914	hyaluronic acid	900:914	hyaluronic acid (HA)	900:919	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	29	located	detected	926:933	arg2	DS					892:893	DS	892:893	DS	892:893	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	29	located	detected	926:933	arg2	HA					917:918	HA	917:918	HA	917:918	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	29	located	detected	926:933	arg2	sulfate					822:828	Heparan sulfate	814:828	Heparan sulfate (HS)/heparin (HP)	814:846	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	29	located	detected	926:933	arg1	O.ran					938:942	O.ran	938:942	O.ran	938:942	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	29	located	detected	926:933	arg2	CS					870:871	CS	870:871	CS	870:871	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	29	located	detected	926:933	arg2	HS					831:832	HS	831:832	HS	831:832	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	14	30	theme	structural	1205:1214	arg1	information					1216:1226	This novel structural information	1194:1226	This novel structural information	1194:1226	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of O.ran.					
33411075	14	31	theme	biological	1304:1313	arg1	activities					1315:1324	the biological activities	1300:1324	the biological activities of O.ran	1300:1333	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of O.ran.					
33411075	14	32	theme	novel	1199:1203	arg1	information					1216:1226	This novel structural information	1194:1226	This novel structural information	1194:1226	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of O.ran.					
33411075	9	33	theme	disaccharide	692:703	arg1	compositions					705:716	their disaccharide compositions	686:716	their disaccharide compositions	686:716	and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
33411075	8	34	attach	isolated	661:668	arg1	O.ran					675:679	O.ran	675:679	O.ran	675:679	In this study, glycosaminoglycans were isolated from O.ran.					
33411075	8	34	attach	isolated	661:668	arg2	glycosaminoglycans					637:654	glycosaminoglycans	637:654	glycosaminoglycans	637:654	In this study, glycosaminoglycans were isolated from O.ran.					
33411075	10	35	theme	chondroitin	849:859	arg1	sulfate					861:867	chondroitin sulfate	849:867	chondroitin sulfate	849:867	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	2	36	theme	Chinese	152:158	arg1	medicine					160:167	a traditional animal-based Chinese medicine	125:167	a traditional animal-based Chinese medicine	125:167	has been widely used as a tonic and a traditional animal-based Chinese medicine.					
33411075	10	37	theme	Heparan	814:820	arg1	sulfate					822:828	Heparan sulfate	814:828	Heparan sulfate (HS)/heparin (HP)	814:846	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	37	theme	Heparan	814:820	arg1	CS					870:871	CS	870:871	CS	870:871	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	37	theme	Heparan	814:820	arg1	HS					831:832	HS	831:832	HS	831:832	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	12	38	from	structures	1053:1062	arg1	chains					1085:1090	their constituent chains	1067:1090	their constituent chains	1067:1090	HS species contain highly acetylated disaccharides, and have various structures in their constituent chains.					
33411075	12	39	theme	various	1045:1051	arg1	structures					1053:1062	various structures	1045:1062	various structures in their constituent chains	1045:1090	HS species contain highly acetylated disaccharides, and have various structures in their constituent chains.					
33411075	2	40	theme	animal-based	139:150	arg1	medicine					160:167	a traditional animal-based Chinese medicine	125:167	a traditional animal-based Chinese medicine	125:167	has been widely used as a tonic and a traditional animal-based Chinese medicine.					
33411075	12	41	theme	acetylated	1010:1019	arg1	disaccharides					1021:1033	highly acetylated disaccharides	1003:1033	highly acetylated disaccharides	1003:1033	HS species contain highly acetylated disaccharides, and have various structures in their constituent chains.					
33411075	5	42	from	ubiquitous	445:454	arg1	mammals					459:465	mammals	459:465	mammals that possess a wide range of biological activities	459:516	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	4	43	theme	biological	222:231	arg1	activities					233:242	numerous biological activities	213:242	numerous biological activities	213:242	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	4	43	theme	biological	222:231	arg1	activities					255:264	activities	255:264	activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic	255:388	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	13	44	theme	heterogeneous	1121:1133	arg1	structure					1135:1143	a heterogeneous structure	1119:1143	a heterogeneous structure with different sulfation patterns and densities	1119:1191	CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities.					
33411075	5	45	contain	possess	472:478	arg2	range					487:491	a wide range	480:491	a wide range of biological activities	480:516	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	5	45	contain	possess	472:478	arg1	mammals					459:465	mammals	459:465	mammals that possess a wide range of biological activities	459:516	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	10	46	theme	hyaluronic	900:909	arg1	acid					911:914	hyaluronic acid	900:914	hyaluronic acid (HA)	900:919	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	10	46	theme	hyaluronic	900:909	arg1	HA					917:918	HA	917:918	HA	917:918	Heparan sulfate (HS)/heparin (HP), chondroitin sulfate (CS)/dermatan sulfate (DS) and hyaluronic acid (HA) were detected in O.ran.					
33411075	5	47	theme	wide	482:485	arg1	range					487:491	a wide range	480:491	a wide range of biological activities	480:516	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	5	48	theme	complex	414:420	arg1	Glycosaminoglycans					391:408	Glycosaminoglycans	391:408	Glycosaminoglycans	391:408	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	5	48	theme	complex	414:420	arg1	polysaccharides					429:443	complex linear polysaccharides	414:443	complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities	414:516	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	9	49	theme	liquid	735:740	arg1	LC-MS-ITTOF					800:810	LC-MS-ITTOF	800:810	LC-MS-ITTOF	800:810	and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
33411075	9	49	theme	liquid	735:740	arg1	spectrometry					786:797	liquid chromatography-ion trap/time-of-flight mass spectrometry	735:797	liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	735:811	and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
33411075	5	50	theme	linear	422:427	arg1	Glycosaminoglycans					391:408	Glycosaminoglycans	391:408	Glycosaminoglycans	391:408	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	5	50	theme	linear	422:427	arg1	polysaccharides					429:443	complex linear polysaccharides	414:443	complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities	414:516	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	12	51	theme	HS	984:985	arg1	species					987:993	HS species	984:993	HS species	984:993	HS species contain highly acetylated disaccharides, and have various structures in their constituent chains.					
33411075	9	52	theme	chromatography-ion	742:759	arg1	LC-MS-ITTOF					800:810	LC-MS-ITTOF	800:810	LC-MS-ITTOF	800:810	and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
33411075	9	52	theme	chromatography-ion	742:759	arg1	spectrometry					786:797	liquid chromatography-ion trap/time-of-flight mass spectrometry	735:797	liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	735:811	and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
33411075	4	53	theme	numerous	213:220	arg1	activities					233:242	numerous biological activities	213:242	numerous biological activities	213:242	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	4	53	theme	numerous	213:220	arg1	activities					255:264	activities	255:264	activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic	255:388	extracts have been reported to have numerous biological activities, including activities that are often associated with mammalian glycosaminoglycans such as anti-inflammatory, antiosteoperotic, and anti-asthmatic.					
33411075	9	54	theme	trap/time-of-flight	761:779	arg1	LC-MS-ITTOF					800:810	LC-MS-ITTOF	800:810	LC-MS-ITTOF	800:810	and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
33411075	9	54	theme	trap/time-of-flight	761:779	arg1	spectrometry					786:797	liquid chromatography-ion trap/time-of-flight mass spectrometry	735:797	liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	735:811	and their disaccharide compositions were analyzed by liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
33411075	13	55	theme	CS/DS	1093:1097	arg1	chains					1099:1104	CS/DS chains	1093:1104	CS/DS chains	1093:1104	CS/DS chains also possess a heterogeneous structure with different sulfation patterns and densities.					
33411075	5	56	theme	ubiquitous	445:454	arg1	Glycosaminoglycans					391:408	Glycosaminoglycans	391:408	Glycosaminoglycans	391:408	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
33411075	5	56	theme	ubiquitous	445:454	arg1	polysaccharides					429:443	complex linear polysaccharides	414:443	complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities	414:516	Glycosaminoglycans are complex linear polysaccharides ubiquitous in mammals that possess a wide range of biological activities.					
32221390	0	0	theme	PGM1	86:89	arg1	isoform					92:98	human phosphoglucomutase-1 (PGM1) isoform 2	58:100	human phosphoglucomutase-1 (PGM1) isoform 2	58:100	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily.					
32221390	4	1	theme	variant	970:976	arg1	structures					942:951	three crystal structures	928:951	three crystal structures of the isoform 2 variant of PGM1	928:984	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	4	1	theme	variant	970:976	arg1	enzyme					1002:1007	a free enzyme	995:1007	a free enzyme	995:1007	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	2	2	theme	nucleotide	602:611	arg1	sugars					613:618	nucleotide sugars	602:618	nucleotide sugars needed for glycan biosynthesis	602:649	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	6	3	theme	common	1377:1382	arg1	ancestor					1384:1391	a common ancestor	1375:1391	a common ancestor of jawed vertebrates	1375:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	2	4	from	important	557:565	arg1	biosynthesis					586:597	the cytoplasmic biosynthesis	570:597	the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis	570:649	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	3	5	from	mutations	685:693	arg1	gene					707:710	the PGM1 gene	698:710	the PGM1 gene	698:710	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	0	6	from	basis	11:15	arg1	isoform					92:98	human phosphoglucomutase-1 (PGM1) isoform 2	58:100	human phosphoglucomutase-1 (PGM1) isoform 2	58:100	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily.					
32221390	1	7	with	superfamily	306:316	arg1	members					323:329	members	323:329	members in all three domains of life	323:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	8	theme	Human	154:158	arg1	phosphoglucomutase					160:177	Human phosphoglucomutase 1	154:179	Human phosphoglucomutase 1 (PGM1)	154:186	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	8	theme	Human	154:158	arg1	PGM1					182:185	PGM1	182:185	PGM1	182:185	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	8	theme	Human	154:158	arg1	enzyme					217:222	an evolutionary conserved enzyme	191:222	an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life	191:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	7	9	theme	human	1617:1621	arg1	organism					1623:1630	the human organism	1613:1630	the human organism	1613:1630	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	3	10	theme	PGM1	826:829	arg1	deficiency					831:840	PGM1 deficiency	826:840	PGM1 deficiency	826:840	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	3	10	theme	PGM1	826:829	arg1	disorder					885:892	a congenital disorder	872:892	a congenital disorder of glycosylation	872:909	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	4	11	theme	crystal	934:940	arg1	structures					942:951	three crystal structures	928:951	three crystal structures of the isoform 2 variant of PGM1	928:984	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	4	11	theme	crystal	934:940	arg1	enzyme					1002:1007	a free enzyme	995:1007	a free enzyme	995:1007	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	7	12	theme	disease-related	1682:1696	arg1	mutations					1707:1715	disease-related missense mutations	1682:1715	disease-related missense mutations in patients in the future	1682:1741	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	1	13	theme	ubiquitous	244:253	arg1	superfamily					306:316	a large enzyme superfamily	291:316	a large enzyme superfamily with members in all three domains of life	291:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	13	theme	ubiquitous	244:253	arg1	α-D-phosphohexomutases					267:288	the ubiquitous and ancient α-D-phosphohexomutases	240:288	the ubiquitous and ancient α-D-phosphohexomutases	240:288	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	3	14	theme	PGM1	722:725	arg1	error					749:753	an inborn error	739:753	an inborn error of metabolism previously classified as a glycogen storage disease	739:819	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	3	14	theme	PGM1	722:725	arg1	deficiency					727:736	PGM1 deficiency	722:736	PGM1 deficiency	722:736	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	2	15	theme	cytoplasmic	574:584	arg1	biosynthesis					586:597	the cytoplasmic biosynthesis	570:597	the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis	570:649	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	7	16	theme	missense	1698:1705	arg1	mutations					1707:1715	disease-related missense mutations	1682:1715	disease-related missense mutations in patients in the future	1682:1741	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	4	17	theme	isoform	960:966	arg1	variant					970:976	the isoform 2 variant	956:976	the isoform 2 variant of PGM1	956:984	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	2	18	theme	glycan	631:636	arg1	biosynthesis					638:649	glycan biosynthesis	631:649	glycan biosynthesis	631:649	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	7	19	theme	mutations	1707:1715	arg1	evaluation					1668:1677	the evaluation	1664:1677	the evaluation of disease-related missense mutations in patients in the future	1664:1741	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	3	20	theme	glycosylation	897:909	arg1	deficiency					831:840	PGM1 deficiency	826:840	PGM1 deficiency	826:840	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	3	20	theme	glycosylation	897:909	arg1	disorder					885:892	a congenital disorder	872:892	a congenital disorder of glycosylation	872:909	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	2	21	theme	bi-directional	380:393	arg1	interconversion					395:409	the bi-directional interconversion	376:409	the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis	376:649	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	1	22	theme	life	355:358	arg1	life					355:358	life	355:358	life	355:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	22	theme	life	355:358	arg1	domains					344:350	all three domains	334:350	all three domains of life	334:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	4	23	with	complex	1016:1022	arg1	substrate					1033:1041	substrate	1033:1041	substrate	1033:1041	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	4	23	with	complex	1016:1022	arg1	product					1047:1053	product	1047:1053	product	1047:1053	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	6	24	theme	duplication	1354:1364	arg1	event					1366:1370	a gene duplication event	1347:1370	a gene duplication event in a common ancestor of jawed vertebrates	1347:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	3	25	theme	congenital	874:883	arg1	deficiency					831:840	PGM1 deficiency	826:840	PGM1 deficiency	826:840	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	3	25	theme	congenital	874:883	arg1	disorder					885:892	a congenital disorder	872:892	a congenital disorder of glycosylation	872:909	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	6	26	theme	event	1366:1370	arg1	results					1336:1342	the results	1332:1342	the results of a gene duplication event in a common ancestor of jawed vertebrates	1332:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	26	theme	event	1366:1370	arg1	significance					1489:1500	functional significance	1478:1500	functional significance in all vertebrates	1478:1519	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	26	theme	event	1366:1370	arg1	PGM1					1294:1297	PGM1	1294:1297	PGM1	1294:1297	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	26	theme	event	1366:1370	arg1	PGM5					1323:1326	the paralogous gene PGM5	1303:1326	the paralogous gene PGM5	1303:1326	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	2	27	from	biosynthesis	586:597	arg1	important					557:565	important	557:565	important	557:565	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	4	28	attach	present	920:926	arg1	complex					1016:1022	complex	1016:1022	complex with its substrate and product	1016:1053	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	4	28	attach	present	920:926	arg2	we					917:918	we	917:918	we	917:918	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	0	29	theme	Structural	0:9	arg1	basis					11:15	Structural basis	0:15	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2	0:100	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily.					
32221390	0	29	theme	Structural	0:9	arg1	member					105:110	a member	103:110	a member of the α-D-phosphohexomutase superfamily	103:151	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily.					
32221390	1	30	theme	ancient	259:265	arg1	superfamily					306:316	a large enzyme superfamily	291:316	a large enzyme superfamily with members in all three domains of life	291:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	30	theme	ancient	259:265	arg1	α-D-phosphohexomutases					267:288	the ubiquitous and ancient α-D-phosphohexomutases	240:288	the ubiquitous and ancient α-D-phosphohexomutases	240:288	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	2	31	theme	sugars	613:618	arg1	biosynthesis					586:597	the cytoplasmic biosynthesis	570:597	the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis	570:649	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	5	32	theme	product	1241:1247	arg1	recognition					1249:1259	G6P product recognition	1237:1259	G6P product recognition	1237:1259	The structures show the longer N-terminal of this PGM1 variant, and the ligand complex structures reveal for the first time the detailed structural basis for both G1P substrate and G6P product recognition by human PGM1.					
32221390	6	33	theme	gene	1349:1352	arg1	event					1366:1370	a gene duplication event	1347:1370	a gene duplication event in a common ancestor of jawed vertebrates	1347:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	34	theme	paralogous	1307:1316	arg1	results					1336:1342	the results	1332:1342	the results of a gene duplication event in a common ancestor of jawed vertebrates	1332:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	34	theme	paralogous	1307:1316	arg1	PGM1					1294:1297	PGM1	1294:1297	PGM1	1294:1297	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	34	theme	paralogous	1307:1316	arg1	PGM5					1323:1326	the paralogous gene PGM5	1303:1326	the paralogous gene PGM5	1303:1326	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	4	35	theme	free	997:1000	arg1	structures					942:951	three crystal structures	928:951	three crystal structures of the isoform 2 variant of PGM1	928:984	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	4	35	theme	free	997:1000	arg1	enzyme					1002:1007	a free enzyme	995:1007	a free enzyme	995:1007	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	5	36	theme	ligand	1128:1133	arg1	structures					1143:1152	the ligand complex structures	1124:1152	the ligand complex structures	1124:1152	The structures show the longer N-terminal of this PGM1 variant, and the ligand complex structures reveal for the first time the detailed structural basis for both G1P substrate and G6P product recognition by human PGM1.					
32221390	3	37	theme	Clinical	652:659	arg1	studies					661:667	Clinical studies	652:667	Clinical studies	652:667	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	7	38	theme	conserved	1564:1572	arg1	isoforms					1601:1608	two equally conserved and functionally important isoforms	1552:1608	two equally conserved and functionally important isoforms in the human organism	1552:1630	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	7	38	theme	conserved	1564:1572	arg1	PGM1					1539:1542	PGM1	1539:1542	PGM1	1539:1542	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	2	39	theme	α-D-glucose	419:429	arg1	reaction					486:493	a reaction	484:493	a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis	484:649	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	2	39	theme	α-D-glucose	419:429	arg1	G6P					478:480	G6P	478:480	G6P	478:480	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	2	39	theme	α-D-glucose	419:429	arg1	essential					503:511	essential	503:511	essential	503:511	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	2	39	theme	α-D-glucose	419:429	arg1	G1P					444:446	G1P	444:446	G1P	444:446	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	2	39	theme	α-D-glucose	419:429	arg1	1-phosphate					431:441	α-D-glucose 1-phosphate	419:441	α-D-glucose 1-phosphate (G1P)	419:447	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	4	40	theme	PGM1	981:984	arg1	variant					970:976	the isoform 2 variant	956:976	the isoform 2 variant of PGM1	956:984	Here we present three crystal structures of the isoform 2 variant of PGM1, both as a free enzyme and in complex with its substrate and product.					
32221390	5	41	theme	complex	1135:1141	arg1	structures					1143:1152	the ligand complex structures	1124:1152	the ligand complex structures	1124:1152	The structures show the longer N-terminal of this PGM1 variant, and the ligand complex structures reveal for the first time the detailed structural basis for both G1P substrate and G6P product recognition by human PGM1.					
32221390	2	42	theme	carbohydrate	524:535	arg1	metabolism					537:546	normal carbohydrate metabolism	517:546	normal carbohydrate metabolism	517:546	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	5	43	theme	first	1169:1173	arg1	time					1175:1178	the first time	1165:1178	the first time	1165:1178	The structures show the longer N-terminal of this PGM1 variant, and the ligand complex structures reveal for the first time the detailed structural basis for both G1P substrate and G6P product recognition by human PGM1.					
32221390	3	44	theme	inborn	742:747	arg1	error					749:753	an inborn error	739:753	an inborn error of metabolism previously classified as a glycogen storage disease	739:819	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	3	44	theme	inborn	742:747	arg1	deficiency					727:736	PGM1 deficiency	722:736	PGM1 deficiency	722:736	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	5	45	theme	human	1264:1268	arg1	PGM1					1270:1273	human PGM1	1264:1273	human PGM1	1264:1273	The structures show the longer N-terminal of this PGM1 variant, and the ligand complex structures reveal for the first time the detailed structural basis for both G1P substrate and G6P product recognition by human PGM1.					
32221390	1	46	theme	large	293:297	arg1	superfamily					306:316	a large enzyme superfamily	291:316	a large enzyme superfamily with members in all three domains of life	291:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	46	theme	large	293:297	arg1	α-D-phosphohexomutases					267:288	the ubiquitous and ancient α-D-phosphohexomutases	240:288	the ubiquitous and ancient α-D-phosphohexomutases	240:288	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	2	47	theme	normal	517:522	arg1	metabolism					537:546	normal carbohydrate metabolism	517:546	normal carbohydrate metabolism	517:546	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	6	48	from	event	1366:1370	arg1	ancestor					1384:1391	a common ancestor	1375:1391	a common ancestor of jawed vertebrates	1375:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	1	49	theme	evolutionary	194:205	arg1	phosphoglucomutase					160:177	Human phosphoglucomutase 1	154:179	Human phosphoglucomutase 1 (PGM1)	154:186	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	49	theme	evolutionary	194:205	arg1	enzyme					217:222	an evolutionary conserved enzyme	191:222	an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life	191:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	50	from	members	323:329	arg1	life					355:358	life	355:358	life	355:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	50	from	members	323:329	arg1	domains					344:350	all three domains	334:350	all three domains of life	334:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	51	theme	enzyme	299:304	arg1	superfamily					306:316	a large enzyme superfamily	291:316	a large enzyme superfamily with members in all three domains of life	291:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	51	theme	enzyme	299:304	arg1	α-D-phosphohexomutases					267:288	the ubiquitous and ancient α-D-phosphohexomutases	240:288	the ubiquitous and ancient α-D-phosphohexomutases	240:288	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	0	52	theme	superfamily	141:151	arg1	member					105:110	a member	103:110	a member of the α-D-phosphohexomutase superfamily	103:151	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily.					
32221390	0	52	theme	superfamily	141:151	arg1	basis					11:15	Structural basis	0:15	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2	0:100	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily.					
32221390	6	53	theme	gene	1318:1321	arg1	results					1336:1342	the results	1332:1342	the results of a gene duplication event in a common ancestor of jawed vertebrates	1332:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	53	theme	gene	1318:1321	arg1	PGM1					1294:1297	PGM1	1294:1297	PGM1	1294:1297	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	53	theme	gene	1318:1321	arg1	PGM5					1323:1326	the paralogous gene PGM5	1303:1326	the paralogous gene PGM5	1303:1326	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	54	from	significance	1489:1500	arg1	vertebrates					1509:1519	all vertebrates	1505:1519	all vertebrates	1505:1519	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	54	from	significance	1489:1500	arg1	ancestor					1384:1391	a common ancestor	1375:1391	a common ancestor of jawed vertebrates	1375:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	2	55	theme	α-D-glucose	453:463	arg1	6-phosphate					465:475	α-D-glucose 6-phosphate	453:475	α-D-glucose 6-phosphate	453:475	PGM1 catalyzes the bi-directional interconversion between α-D-glucose 1-phosphate (G1P) and α-D-glucose 6-phosphate (G6P), a reaction that is essential for normal carbohydrate metabolism and also important in the cytoplasmic biosynthesis of nucleotide sugars needed for glycan biosynthesis.					
32221390	0	56	theme	α-D-phosphohexomutase	119:139	arg1	superfamily					141:151	the α-D-phosphohexomutase superfamily	115:151	the α-D-phosphohexomutase superfamily	115:151	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily.					
32221390	7	57	from	evaluation	1668:1677	arg1	patients					1720:1727	patients	1720:1727	patients in the future	1720:1741	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	5	58	theme	G1P	1219:1221	arg1	substrate					1223:1231	G1P substrate	1219:1231	G1P substrate	1219:1231	The structures show the longer N-terminal of this PGM1 variant, and the ligand complex structures reveal for the first time the detailed structural basis for both G1P substrate and G6P product recognition by human PGM1.					
32221390	3	59	theme	metabolism	758:767	arg1	error					749:753	an inborn error	739:753	an inborn error of metabolism previously classified as a glycogen storage disease	739:819	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	3	59	theme	metabolism	758:767	arg1	deficiency					727:736	PGM1 deficiency	722:736	PGM1 deficiency	722:736	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	0	60	dep	substrate	21:29	arg1	recognition					43:53	recognition	43:53	recognition	43:53	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily.					
32221390	6	61	theme	functional	1478:1487	arg1	significance					1489:1500	functional significance	1478:1500	functional significance in all vertebrates	1478:1519	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	7	62	from	patients	1720:1727	arg1	future					1736:1741	future	1736:1741	future	1736:1741	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	7	62	from	patients	1720:1727	arg1	evaluation					1668:1677	the evaluation	1664:1677	the evaluation of disease-related missense mutations in patients in the future	1664:1741	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	6	63	theme	vertebrates	1402:1412	arg1	ancestor					1384:1391	a common ancestor	1375:1391	a common ancestor of jawed vertebrates	1375:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	1	64	theme	conserved	207:215	arg1	phosphoglucomutase					160:177	Human phosphoglucomutase 1	154:179	Human phosphoglucomutase 1 (PGM1)	154:186	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	1	64	theme	conserved	207:215	arg1	enzyme					217:222	an evolutionary conserved enzyme	191:222	an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life	191:358	Human phosphoglucomutase 1 (PGM1) is an evolutionary conserved enzyme that belongs to the ubiquitous and ancient α-D-phosphohexomutases, a large enzyme superfamily with members in all three domains of life.					
32221390	7	65	theme	important	1591:1599	arg1	isoforms					1601:1608	two equally conserved and functionally important isoforms	1552:1608	two equally conserved and functionally important isoforms in the human organism	1552:1630	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	7	65	theme	important	1591:1599	arg1	PGM1					1539:1542	PGM1	1539:1542	PGM1	1539:1542	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	6	66	theme	PGM1	1443:1446	arg1	isoforms					1448:1455	both PGM1 isoforms	1438:1455	both PGM1 isoforms	1438:1455	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	7	67	from	isoforms	1601:1608	arg1	organism					1623:1630	the human organism	1613:1630	the human organism	1613:1630	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	7	68	from	mutations	1707:1715	arg1	patients					1720:1727	patients	1720:1727	patients in the future	1720:1741	Our finding that PGM1 encodes two equally conserved and functionally important isoforms in the human organism should be taken into account in the evaluation of disease-related missense mutations in patients in the future.					
32221390	6	69	theme	jawed	1396:1400	arg1	vertebrates					1402:1412	jawed vertebrates	1396:1412	jawed vertebrates	1396:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	3	70	theme	storage	805:811	arg1	disease					813:819	a glycogen storage disease	794:819	a glycogen storage disease	794:819	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	0	71	theme	phosphoglucomutase-1	64:83	arg1	isoform					92:98	human phosphoglucomutase-1 (PGM1) isoform 2	58:100	human phosphoglucomutase-1 (PGM1) isoform 2	58:100	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily.					
32221390	6	72	from	results	1336:1342	arg1	vertebrates					1509:1519	all vertebrates	1505:1519	all vertebrates	1505:1519	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	72	from	results	1336:1342	arg1	ancestor					1384:1391	a common ancestor	1375:1391	a common ancestor of jawed vertebrates	1375:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	73	from	ancestor	1384:1391	arg1	results					1336:1342	the results	1332:1342	the results of a gene duplication event in a common ancestor of jawed vertebrates	1332:1412	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	73	from	ancestor	1384:1391	arg1	significance					1489:1500	functional significance	1478:1500	functional significance in all vertebrates	1478:1519	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	73	from	ancestor	1384:1391	arg1	PGM1					1294:1297	PGM1	1294:1297	PGM1	1294:1297	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	6	73	from	ancestor	1384:1391	arg1	PGM5					1323:1326	the paralogous gene PGM5	1303:1326	the paralogous gene PGM5	1303:1326	We also show that PGM1 and the paralogous gene PGM5 are the results of a gene duplication event in a common ancestor of jawed vertebrates, and, importantly, that both PGM1 isoforms are conserved and of functional significance in all vertebrates.					
32221390	5	74	theme	G6P	1237:1239	arg1	recognition					1249:1259	G6P product recognition	1237:1259	G6P product recognition	1237:1259	The structures show the longer N-terminal of this PGM1 variant, and the ligand complex structures reveal for the first time the detailed structural basis for both G1P substrate and G6P product recognition by human PGM1.					
32221390	0	75	theme	human	58:62	arg1	isoform					92:98	human phosphoglucomutase-1 (PGM1) isoform 2	58:100	human phosphoglucomutase-1 (PGM1) isoform 2	58:100	Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily.					
32221390	3	76	theme	PGM1	702:705	arg1	gene					707:710	the PGM1 gene	698:710	the PGM1 gene	698:710	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	5	77	theme	detailed	1184:1191	arg1	basis					1204:1208	the detailed structural basis	1180:1208	the detailed structural basis for both G1P substrate and G6P product recognition	1180:1259	The structures show the longer N-terminal of this PGM1 variant, and the ligand complex structures reveal for the first time the detailed structural basis for both G1P substrate and G6P product recognition by human PGM1.					
32221390	3	78	theme	glycogen	796:803	arg1	disease					813:819	a glycogen storage disease	794:819	a glycogen storage disease	794:819	Clinical studies have shown that mutations in the PGM1 gene may cause PGM1 deficiency, an inborn error of metabolism previously classified as a glycogen storage disease, and PGM1 deficiency was recently also shown to be a congenital disorder of glycosylation.					
32221390	5	79	theme	structural	1193:1202	arg1	basis					1204:1208	the detailed structural basis	1180:1208	the detailed structural basis for both G1P substrate and G6P product recognition	1180:1259	The structures show the longer N-terminal of this PGM1 variant, and the ligand complex structures reveal for the first time the detailed structural basis for both G1P substrate and G6P product recognition by human PGM1.					
32221390	5	80	theme	PGM1	1106:1109	arg1	variant					1111:1117	this PGM1 variant	1101:1117	this PGM1 variant	1101:1117	The structures show the longer N-terminal of this PGM1 variant, and the ligand complex structures reveal for the first time the detailed structural basis for both G1P substrate and G6P product recognition by human PGM1.					
34044956	0	0	theme	different	103:111	arg1	methods					113:119	different methods	103:119	different methods	103:119	Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods.					
34044956	2	1	from	properties	440:449	arg1	methods					548:554	different extraction methods	527:554	different extraction methods	527:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	6	2	theme	better	1326:1331	arg1	activities					1361:1370	better hydroxyl radical scavenging activities	1326:1370	better hydroxyl radical scavenging activities	1326:1370	Among these BSPs, BSPs-A exhibited better DPPH and ABTS radical scavenging activities and reducing power, whereas BSPs-H showed better hydroxyl radical scavenging activities.					
34044956	4	3	theme	reticular	1070:1078	arg1	structure					1093:1101	reticular and lamellar structure	1070:1101	reticular and lamellar structure	1070:1101	By the above-mentioned four kinds of extraction methods, the resultant BSPs exhibited various degrees of reticular and lamellar structure.					
34044956	3	4	theme	bond	858:861	arg1	weights					809:815	different molecular weights	789:815	different molecular weights	789:815	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	4	theme	bond	858:861	arg1	compositions					863:874	glycosidic bond compositions	847:874	glycosidic bond compositions	847:874	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	4	5	theme	extraction	1002:1011	arg1	methods					1013:1019	extraction methods	1002:1019	extraction methods	1002:1019	By the above-mentioned four kinds of extraction methods, the resultant BSPs exhibited various degrees of reticular and lamellar structure.					
34044956	2	6	from	properties	464:473	arg1	methods					548:554	different extraction methods	527:554	different extraction methods	527:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	3	7	theme	alkali-assisted	658:672	arg1	extraction					674:683	alkali-assisted extraction	658:683	alkali-assisted extraction (BSPs-A)	658:692	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	7	theme	alkali-assisted	658:672	arg1	BSPs-A					686:691	BSPs-A	686:691	BSPs-A	686:691	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	4	8	theme	structure	1093:1101	arg1	degrees					1059:1065	various degrees	1051:1065	various degrees of reticular and lamellar structure	1051:1101	By the above-mentioned four kinds of extraction methods, the resultant BSPs exhibited various degrees of reticular and lamellar structure.					
34044956	3	9	theme	glycosidic	847:856	arg1	weights					809:815	different molecular weights	789:815	different molecular weights	789:815	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	9	theme	glycosidic	847:856	arg1	compositions					863:874	glycosidic bond compositions	847:874	glycosidic bond compositions	847:874	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	1	10	dep	pharmacy	293:300	arg1	science					302:308	science	302:308	science	302:308	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	3	11	theme	Bletilla	597:604	arg1	striata					606:612	Bletilla striata	597:612	Bletilla striata	597:612	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	1	12	theme	food	281:284	arg1	chain					286:290	food chain	281:290	food chain	281:290	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	3	13	theme	water	703:707	arg1	BSPs-B					721:726	BSPs-B	721:726	BSPs-B	721:726	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	13	theme	water	703:707	arg1	extraction					709:718	boiling water extraction	695:718	boiling water extraction (BSPs-B)	695:727	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	1	14	with	effective	166:174	arg1	applications					253:264	important applications	243:264	important applications in the area of food chain, pharmacy science, and health care	243:325	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	1	15	theme	chain	286:290	arg1	area					273:276	the area	269:276	the area of food chain, pharmacy science, and health care	269:325	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	2	16	theme	extraction	537:546	arg1	methods					548:554	different extraction methods	527:554	different extraction methods	527:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	3	17	theme	ultrasonic-assisted	734:752	arg1	BSPs-U					766:771	BSPs-U	766:771	BSPs-U	766:771	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	17	theme	ultrasonic-assisted	734:752	arg1	extraction					754:763	ultrasonic-assisted extraction	734:763	ultrasonic-assisted extraction (BSPs-U)	734:772	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	4	18	theme	lamellar	1084:1091	arg1	structure					1093:1101	reticular and lamellar structure	1070:1101	reticular and lamellar structure	1070:1101	By the above-mentioned four kinds of extraction methods, the resultant BSPs exhibited various degrees of reticular and lamellar structure.					
34044956	5	19	theme	BSPs	1112:1115	arg1	solutions					1117:1125	All the BSPs solutions	1104:1125	All the BSPs solutions	1104:1125	All the BSPs solutions exhibited shear-thinning behavior with the increase of the shear rate.					
34044956	2	20	theme	different	527:535	arg1	methods					548:554	different extraction methods	527:554	different extraction methods	527:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	5	21	theme	shear-thinning	1137:1150	arg1	behavior					1152:1159	shear-thinning behavior	1137:1159	shear-thinning behavior	1137:1159	All the BSPs solutions exhibited shear-thinning behavior with the increase of the shear rate.					
34044956	6	22	theme	reducing	1288:1295	arg1	power					1297:1301	reducing power	1288:1301	reducing power	1288:1301	Among these BSPs, BSPs-A exhibited better DPPH and ABTS radical scavenging activities and reducing power, whereas BSPs-H showed better hydroxyl radical scavenging activities.					
34044956	2	23	theme	rheological	452:462	arg1	properties					464:473	rheological properties	452:473	rheological properties	452:473	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	3	24	theme	same	911:914	arg1	spectra					919:925	the same IR spectra	907:925	the same IR spectra characteristic and thermal stability	907:962	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	24	theme	same	911:914	arg1	characteristic					927:940	characteristic	927:940	characteristic	927:940	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	24	theme	same	911:914	arg1	stability					954:962	thermal stability	946:962	thermal stability	946:962	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	5	25	theme	rate	1192:1195	arg1	increase					1170:1177	the increase	1166:1177	the increase of the shear rate	1166:1195	All the BSPs solutions exhibited shear-thinning behavior with the increase of the shear rate.					
34044956	4	26	theme	various	1051:1057	arg1	degrees					1059:1065	various degrees	1051:1065	various degrees of reticular and lamellar structure	1051:1101	By the above-mentioned four kinds of extraction methods, the resultant BSPs exhibited various degrees of reticular and lamellar structure.					
34044956	3	27	theme	crude	561:565	arg1	polysaccharides					567:581	The crude polysaccharides	557:581	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods	557:780	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	28	theme	IR	916:917	arg1	spectra					919:925	the same IR spectra	907:925	the same IR spectra characteristic and thermal stability	907:962	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	28	theme	IR	916:917	arg1	characteristic					927:940	characteristic	927:940	characteristic	927:940	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	28	theme	IR	916:917	arg1	stability					954:962	thermal stability	946:962	thermal stability	946:962	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	0	29	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods.					
34044956	1	30	dep	anti-inflammatory	180:196	arg1	scavenging					226:235	scavenging	226:235	scavenging	226:235	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	0	31	theme	antioxidant	32:42	arg1	activities					44:53	antioxidant activities	32:53	antioxidant activities	32:53	Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods.					
34044956	1	32	theme	pharmacy	293:300	arg1	area					273:276	the area	269:276	the area of food chain, pharmacy science, and health care	269:325	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	1	33	theme	Bletilla	122:129	arg1	BSPs					156:159	BSPs	156:159	BSPs	156:159	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	1	33	theme	Bletilla	122:129	arg1	polysaccharides					139:153	Bletilla striata polysaccharides	122:153	Bletilla striata polysaccharides (BSPs)	122:160	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	6	34	theme	scavenging	1262:1271	arg1	activities					1273:1282	better DPPH and ABTS radical scavenging activities	1233:1282	better DPPH and ABTS radical scavenging activities	1233:1282	Among these BSPs, BSPs-A exhibited better DPPH and ABTS radical scavenging activities and reducing power, whereas BSPs-H showed better hydroxyl radical scavenging activities.					
34044956	2	35	theme	physicochemical	424:438	arg1	properties					440:449	physicochemical properties	424:449	physicochemical properties	424:449	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	4	36	theme	resultant	1026:1034	arg1	BSPs					1036:1039	the resultant BSPs	1022:1039	the resultant BSPs	1022:1039	By the above-mentioned four kinds of extraction methods, the resultant BSPs exhibited various degrees of reticular and lamellar structure.					
34044956	1	37	theme	striata	131:137	arg1	BSPs					156:159	BSPs	156:159	BSPs	156:159	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	1	37	theme	striata	131:137	arg1	polysaccharides					139:153	Bletilla striata polysaccharides	122:153	Bletilla striata polysaccharides (BSPs)	122:160	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	2	38	theme	antioxidant	491:501	arg1	activities					503:512	associated antioxidant activities	480:512	associated antioxidant activities of BSPs from different extraction methods	480:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	2	39	from	activities	503:512	arg1	methods					548:554	different extraction methods	527:554	different extraction methods	527:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	3	40	theme	hot	627:629	arg1	BSPs-H					649:654	BSPs-H	649:654	BSPs-H	649:654	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	40	theme	hot	627:629	arg1	extraction					637:646	the hot water extraction	623:646	the hot water extraction (BSPs-H)	623:655	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	41	theme	thermal	946:952	arg1	spectra					919:925	the same IR spectra	907:925	the same IR spectra characteristic and thermal stability	907:962	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	41	theme	thermal	946:952	arg1	stability					954:962	thermal stability	946:962	thermal stability	946:962	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	2	42	theme	associated	480:489	arg1	activities					503:512	associated antioxidant activities	480:512	associated antioxidant activities of BSPs from different extraction methods	480:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	3	43	theme	different	789:797	arg1	weights					809:815	different molecular weights	789:815	different molecular weights	789:815	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	43	theme	different	789:797	arg1	potentials					886:895	zeta potentials	881:895	zeta potentials	881:895	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	43	theme	different	789:797	arg1	compositions					833:844	monosaccharide compositions	818:844	monosaccharide compositions	818:844	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	43	theme	different	789:797	arg1	compositions					863:874	glycosidic bond compositions	847:874	glycosidic bond compositions	847:874	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	44	theme	water	631:635	arg1	BSPs-H					649:654	BSPs-H	649:654	BSPs-H	649:654	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	44	theme	water	631:635	arg1	extraction					637:646	the hot water extraction	623:646	the hot water extraction (BSPs-H)	623:655	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	4	45	theme	above-mentioned	972:986	arg1	kinds					993:997	the above-mentioned four kinds	968:997	the above-mentioned four kinds of extraction methods	968:1019	By the above-mentioned four kinds of extraction methods, the resultant BSPs exhibited various degrees of reticular and lamellar structure.					
34044956	0	46	theme	striata	67:73	arg1	polysaccharide					75:88	Bletilla striata polysaccharide	58:88	Bletilla striata polysaccharide	58:88	Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods.					
34044956	1	47	theme	health	315:320	arg1	care					322:325	health care	315:325	health care	315:325	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	6	48	theme	scavenging	1350:1359	arg1	activities					1361:1370	better hydroxyl radical scavenging activities	1326:1370	better hydroxyl radical scavenging activities	1326:1370	Among these BSPs, BSPs-A exhibited better DPPH and ABTS radical scavenging activities and reducing power, whereas BSPs-H showed better hydroxyl radical scavenging activities.					
34044956	2	49	theme	BSPs	517:520	arg1	properties					440:449	physicochemical properties	424:449	physicochemical properties	424:449	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	2	49	theme	BSPs	517:520	arg1	interplay					374:382	the interplay	370:382	the interplay between the polysaccharides' formation	370:421	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	2	49	theme	BSPs	517:520	arg1	properties					464:473	rheological properties	452:473	rheological properties	452:473	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	2	49	theme	BSPs	517:520	arg1	activities					503:512	associated antioxidant activities	480:512	associated antioxidant activities of BSPs from different extraction methods	480:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	6	50	theme	ABTS	1249:1252	arg1	scavenging					1262:1271	ABTS radical scavenging	1249:1271	ABTS radical scavenging	1249:1271	Among these BSPs, BSPs-A exhibited better DPPH and ABTS radical scavenging activities and reducing power, whereas BSPs-H showed better hydroxyl radical scavenging activities.					
34044956	2	51	from	interplay	374:382	arg1	methods					548:554	different extraction methods	527:554	different extraction methods	527:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	0	52	theme	Bletilla	58:65	arg1	polysaccharide					75:88	Bletilla striata polysaccharide	58:88	Bletilla striata polysaccharide	58:88	Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods.					
34044956	1	53	theme	care	322:325	arg1	area					273:276	the area	269:276	the area of food chain, pharmacy science, and health care	269:325	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	6	54	theme	radical	1342:1348	arg1	activities					1361:1370	better hydroxyl radical scavenging activities	1326:1370	better hydroxyl radical scavenging activities	1326:1370	Among these BSPs, BSPs-A exhibited better DPPH and ABTS radical scavenging activities and reducing power, whereas BSPs-H showed better hydroxyl radical scavenging activities.					
34044956	3	55	dep	spectra	919:925	arg1	spectra					919:925	the same IR spectra	907:925	the same IR spectra characteristic and thermal stability	907:962	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	55	dep	spectra	919:925	arg1	characteristic					927:940	characteristic	927:940	characteristic	927:940	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	55	dep	spectra	919:925	arg1	stability					954:962	thermal stability	946:962	thermal stability	946:962	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	56	theme	zeta	881:884	arg1	weights					809:815	different molecular weights	789:815	different molecular weights	789:815	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	56	theme	zeta	881:884	arg1	potentials					886:895	zeta potentials	881:895	zeta potentials	881:895	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	6	57	theme	hydroxyl	1333:1340	arg1	activities					1361:1370	better hydroxyl radical scavenging activities	1326:1370	better hydroxyl radical scavenging activities	1326:1370	Among these BSPs, BSPs-A exhibited better DPPH and ABTS radical scavenging activities and reducing power, whereas BSPs-H showed better hydroxyl radical scavenging activities.					
34044956	3	58	theme	boiling	695:701	arg1	BSPs-B					721:726	BSPs-B	721:726	BSPs-B	721:726	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	58	theme	boiling	695:701	arg1	extraction					709:718	boiling water extraction	695:718	boiling water extraction (BSPs-B)	695:727	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	6	59	theme	DPPH	1240:1243	arg1	activities					1273:1282	better DPPH and ABTS radical scavenging activities	1233:1282	better DPPH and ABTS radical scavenging activities	1233:1282	Among these BSPs, BSPs-A exhibited better DPPH and ABTS radical scavenging activities and reducing power, whereas BSPs-H showed better hydroxyl radical scavenging activities.					
34044956	6	60	theme	radical	1254:1260	arg1	scavenging					1262:1271	ABTS radical scavenging	1249:1271	ABTS radical scavenging	1249:1271	Among these BSPs, BSPs-A exhibited better DPPH and ABTS radical scavenging activities and reducing power, whereas BSPs-H showed better hydroxyl radical scavenging activities.					
34044956	1	61	theme	important	243:251	arg1	applications					253:264	important applications	243:264	important applications in the area of food chain, pharmacy science, and health care	243:325	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	1	62	from	applications	253:264	arg1	area					273:276	the area	269:276	the area of food chain, pharmacy science, and health care	269:325	Bletilla striata polysaccharides (BSPs) are effective for anti-inflammatory, detumescence, and radicals scavenging, with important applications in the area of food chain, pharmacy science, and health care.					
34044956	6	63	theme	better	1233:1238	arg1	DPPH					1240:1243	better DPPH	1233:1243	better DPPH	1233:1243	Among these BSPs, BSPs-A exhibited better DPPH and ABTS radical scavenging activities and reducing power, whereas BSPs-H showed better hydroxyl radical scavenging activities.					
34044956	5	64	theme	shear	1186:1190	arg1	rate					1192:1195	the shear rate	1182:1195	the shear rate	1182:1195	All the BSPs solutions exhibited shear-thinning behavior with the increase of the shear rate.					
34044956	2	65	from	methods	548:554	arg1	BSPs					517:520	BSPs	517:520	BSPs from different extraction methods	517:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	2	65	from	methods	548:554	arg1	properties					440:449	physicochemical properties	424:449	physicochemical properties	424:449	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	2	65	from	methods	548:554	arg1	interplay					374:382	the interplay	370:382	the interplay between the polysaccharides' formation	370:421	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	2	65	from	methods	548:554	arg1	properties					464:473	rheological properties	452:473	rheological properties	452:473	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	2	65	from	methods	548:554	arg1	activities					503:512	associated antioxidant activities	480:512	associated antioxidant activities of BSPs from different extraction methods	480:554	In this study, we comprehensively studied the interplay between the polysaccharides' formation, physicochemical properties, rheological properties, and associated antioxidant activities of BSPs from different extraction methods.					
34044956	3	66	dep	extraction	637:646	arg1	methods					774:780	methods	774:780	methods	774:780	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	0	67	theme	polysaccharide	75:88	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods.					
34044956	0	67	theme	polysaccharide	75:88	arg1	activities					44:53	antioxidant activities	32:53	antioxidant activities	32:53	Structural characterization and antioxidant activities of Bletilla striata polysaccharide extracted by different methods.					
34044956	4	68	theme	methods	1013:1019	arg1	kinds					993:997	the above-mentioned four kinds	968:997	the above-mentioned four kinds of extraction methods	968:1019	By the above-mentioned four kinds of extraction methods, the resultant BSPs exhibited various degrees of reticular and lamellar structure.					
34044956	3	69	theme	monosaccharide	818:831	arg1	weights					809:815	different molecular weights	789:815	different molecular weights	789:815	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	69	theme	monosaccharide	818:831	arg1	compositions					833:844	monosaccharide compositions	818:844	monosaccharide compositions	818:844	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	70	theme	molecular	799:807	arg1	weights					809:815	different molecular weights	789:815	different molecular weights	789:815	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	70	theme	molecular	799:807	arg1	potentials					886:895	zeta potentials	881:895	zeta potentials	881:895	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	70	theme	molecular	799:807	arg1	compositions					833:844	monosaccharide compositions	818:844	monosaccharide compositions	818:844	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
34044956	3	70	theme	molecular	799:807	arg1	compositions					863:874	glycosidic bond compositions	847:874	glycosidic bond compositions	847:874	The crude polysaccharides obtained from Bletilla striata by using the hot water extraction (BSPs-H), alkali-assisted extraction (BSPs-A), boiling water extraction (BSPs-B), and ultrasonic-assisted extraction (BSPs-U) methods showed different molecular weights, monosaccharide compositions, glycosidic bond compositions, and zeta potentials, but with the same IR spectra characteristic and thermal stability.					
33352114	0	0	from	bending	10:16	arg1	portion					80:86	the misfold-recognizing portion	56:86	the misfold-recognizing portion of UDP-glucose	56:101	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.					
33352114	4	1	theme	monomeric	698:706	arg1	in vitro					713:720	monomeric UGGT in vitro	698:720	monomeric UGGT in vitro	698:720	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	2	2	theme	domain	371:376	arg1	CtUGGT-ΔTRXL2					390:402	CtUGGT-ΔTRXL2	390:402	CtUGGT-ΔTRXL2	390:402	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	2	2	theme	domain	371:376	arg1	truncation					378:387	its TRXL2 domain truncation	361:387	its TRXL2 domain truncation (CtUGGT-ΔTRXL2)	361:403	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	6	3	theme	distances	879:887	arg1	distributions					862:874	Frequency distributions	852:874	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures	852:999	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	0	4	theme	misfold-recognizing	60:78	arg1	portion					80:86	the misfold-recognizing portion	56:86	the misfold-recognizing portion of UDP-glucose	56:101	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.					
33352114	6	5	theme	glycoprotein	897:908	arg1	residues					910:917	glycoprotein residues	897:917	glycoprotein residues	897:917	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	7	6	theme	recognition	1139:1149	arg1	model					1151:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	Our data support a "one-size-fits-all adjustable spanner" UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.					
33352114	4	7	gly	glycoprotein	603:614	arg1	glycoprotein					603:614	the same glycoprotein	594:614	the same glycoprotein	594:614	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	2	8	theme	TRXL2	365:369	arg1	CtUGGT-ΔTRXL2					390:402	CtUGGT-ΔTRXL2	390:402	CtUGGT-ΔTRXL2	390:402	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	2	8	theme	TRXL2	365:369	arg1	truncation					378:387	its TRXL2 domain truncation	361:387	its TRXL2 domain truncation (CtUGGT-ΔTRXL2)	361:403	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	7	9	theme	substrate	1129:1137	arg1	model					1151:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	Our data support a "one-size-fits-all adjustable spanner" UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.					
33352114	0	10	theme	UDP-glucose	91:101	arg1	portion					80:86	the misfold-recognizing portion	56:86	the misfold-recognizing portion of UDP-glucose	56:101	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.					
33352114	0	11	from	Clamping	0:7	arg1	portion					80:86	the misfold-recognizing portion	56:86	the misfold-recognizing portion of UDP-glucose	56:101	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.					
33352114	1	12	gly	glycoproteins	206:218	arg1	glycoproteins					206:218	misfolded glycoproteins	196:218	misfolded glycoproteins for ER retention	196:235	UDP-glucose:glycoprotein glucosyltransferase (UGGT) flags misfolded glycoproteins for ER retention.					
33352114	6	13	gly	glycosylation	946:958	arg2	sites					960:964	their closest N-linked glycosylation sites	923:964	their closest N-linked glycosylation sites	923:964	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	6	14	gly	glycoprotein	897:908	arg1	glycoprotein					897:908	glycoprotein residues	897:917	glycoprotein residues	897:917	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	4	15	theme	same	598:601	arg1	glycoprotein					603:614	the same glycoprotein	594:614	the same glycoprotein	594:614	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	6	16	theme	glycoprotein	969:980	arg1	structures					990:999	glycoprotein crystal structures	969:999	glycoprotein crystal structures	969:999	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	0	17	from	motions	45:51	arg1	portion					80:86	the misfold-recognizing portion	56:86	the misfold-recognizing portion of UDP-glucose	56:101	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.					
33352114	4	18	theme	misfold	753:759	arg1	recognition					738:748	recognition	738:748	recognition of misfold at that site	738:772	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	6	19	theme	closest	929:935	arg1	sites					960:964	their closest N-linked glycosylation sites	923:964	their closest N-linked glycosylation sites	923:964	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	3	20	theme	twisting	514:521	arg1	movements					536:544	twisting inter-domain movements	514:544	twisting inter-domain movements	514:544	CtUGGT molecular dynamics (MD) simulations capture extended conformations and reveal clamping, bending, and twisting inter-domain movements.					
33352114	0	21	theme	Glycoprotein	104:115	arg1	glucosyltransferase					117:135	Glycoprotein glucosyltransferase	104:135	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.	0:136	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.					
33352114	7	22	theme	adjustable	1104:1113	arg1	model					1151:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	Our data support a "one-size-fits-all adjustable spanner" UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.					
33352114	3	23	theme	dynamics	423:430	arg1	simulations					437:447	CtUGGT molecular dynamics (MD) simulations	406:447	CtUGGT molecular dynamics (MD) simulations	406:447	CtUGGT molecular dynamics (MD) simulations capture extended conformations and reveal clamping, bending, and twisting inter-domain movements.					
33352114	7	24	theme	spanner	1115:1121	arg1	model					1151:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	Our data support a "one-size-fits-all adjustable spanner" UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.					
33352114	6	25	gly	glycoprotein	969:980	arg1	glycoprotein					969:980	glycoprotein crystal structures	969:999	glycoprotein crystal structures	969:999	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	2	26	theme	UGGT	306:309	arg1	structures					256:265	crystal structures	248:265	crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2)	248:403	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	3	27	theme	extended	457:464	arg1	conformations					466:478	extended conformations	457:478	extended conformations	457:478	CtUGGT molecular dynamics (MD) simulations capture extended conformations and reveal clamping, bending, and twisting inter-domain movements.					
33352114	5	28	theme	MD	788:789	arg1	simulations					791:801	the MD simulations	784:801	the MD simulations	784:801	Based on the MD simulations, we estimate the Parodi limit as around 70-80 Å.					
33352114	6	29	theme	Frequency	852:860	arg1	distributions					862:874	Frequency distributions	852:874	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures	852:999	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	2	30	theme	double-cysteine	332:346	arg1	mutants					348:354	two CtUGGT double-cysteine mutants	321:354	two CtUGGT double-cysteine mutants	321:354	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	1	31	theme	misfolded	196:204	arg1	glycoproteins					206:218	misfolded glycoproteins	196:218	misfolded glycoproteins for ER retention	196:235	UDP-glucose:glycoprotein glucosyltransferase (UGGT) flags misfolded glycoproteins for ER retention.					
33352114	3	32	theme	CtUGGT	406:411	arg1	simulations					437:447	CtUGGT molecular dynamics (MD) simulations	406:447	CtUGGT molecular dynamics (MD) simulations	406:447	CtUGGT molecular dynamics (MD) simulations capture extended conformations and reveal clamping, bending, and twisting inter-domain movements.					
33352114	6	33	theme	crystal	982:988	arg1	structures					990:999	glycoprotein crystal structures	969:999	glycoprotein crystal structures	969:999	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	7	34	theme	one-size-fits-all	1086:1102	arg1	model					1151:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	Our data support a "one-size-fits-all adjustable spanner" UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.					
33352114	2	35	theme	CtUGGT	325:330	arg1	mutants					348:354	two CtUGGT double-cysteine mutants	321:354	two CtUGGT double-cysteine mutants	321:354	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	7	36	theme	essential	1166:1174	arg1	role					1176:1179	an essential role	1163:1179	an essential role for the UGGT TRXL2 domain	1163:1205	Our data support a "one-size-fits-all adjustable spanner" UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.					
33352114	4	37	theme	limit	563:567	arg1	"					568:568	"Parodi limit"	555:568	"Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site	555:772	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	4	38	from	site	769:772	arg1	recognition					738:748	recognition	738:748	recognition of misfold at that site	738:772	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	4	39	theme	misfolding	634:643	arg1	glycan					661:666	an N-linked glycan	649:666	an N-linked glycan	649:666	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	4	39	theme	misfolding	634:643	arg1	misfolding					634:643	misfolding	634:643	misfolding	634:643	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	4	39	theme	misfolding	634:643	arg1	site					626:629	a site	624:629	a site of misfolding	624:643	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	6	40	from	residues	910:917	arg1	structures					990:999	glycoprotein crystal structures	969:999	glycoprotein crystal structures	969:999	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	4	41	theme	maximum	574:580	arg1	distance					582:589	the maximum distance	570:589	the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan	570:666	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	6	42	from	sites	960:964	arg1	structures					990:999	glycoprotein crystal structures	969:999	glycoprotein crystal structures	969:999	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	3	43	theme	inter-domain	523:534	arg1	movements					536:544	twisting inter-domain movements	514:544	twisting inter-domain movements	514:544	CtUGGT molecular dynamics (MD) simulations capture extended conformations and reveal clamping, bending, and twisting inter-domain movements.					
33352114	1	44	theme	ER	224:225	arg1	retention					227:235	ER retention	224:235	ER retention	224:235	UDP-glucose:glycoprotein glucosyltransferase (UGGT) flags misfolded glycoproteins for ER retention.					
33352114	0	45	theme	twisting	23:30	arg1	motions					45:51	twisting inter-domain motions	23:51	twisting inter-domain motions	23:51	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.					
33352114	4	46	dep	recognition	738:748	arg1	response					726:733	response	726:733	response	726:733	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	7	47	theme	TRXL2	1194:1198	arg1	domain					1200:1205	the UGGT TRXL2 domain	1185:1205	the UGGT TRXL2 domain	1185:1205	Our data support a "one-size-fits-all adjustable spanner" UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.					
33352114	5	48	theme	Parodi	820:825	arg1	limit					827:831	the Parodi limit	816:831	the Parodi limit as around 70-80 Å	816:849	Based on the MD simulations, we estimate the Parodi limit as around 70-80 Å.					
33352114	3	49	theme	MD	433:434	arg1	simulations					437:447	CtUGGT molecular dynamics (MD) simulations	406:447	CtUGGT molecular dynamics (MD) simulations	406:447	CtUGGT molecular dynamics (MD) simulations capture extended conformations and reveal clamping, bending, and twisting inter-domain movements.					
33352114	6	50	theme	glycosylation	946:958	arg1	sites					960:964	their closest N-linked glycosylation sites	923:964	their closest N-linked glycosylation sites	923:964	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	2	51	theme	crystal	248:254	arg1	structures					256:265	crystal structures	248:265	crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2)	248:403	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	4	52	from	distance	582:589	arg1	glycoprotein					603:614	the same glycoprotein	594:614	the same glycoprotein	594:614	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	3	53	theme	molecular	413:421	arg1	simulations					437:447	CtUGGT molecular dynamics (MD) simulations	406:447	CtUGGT molecular dynamics (MD) simulations	406:447	CtUGGT molecular dynamics (MD) simulations capture extended conformations and reveal clamping, bending, and twisting inter-domain movements.					
33352114	6	54	theme	N-linked	937:944	arg1	sites					960:964	their closest N-linked glycosylation sites	923:964	their closest N-linked glycosylation sites	923:964	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	4	55	dep	distance	582:589	arg1	"					568:568	"Parodi limit"	555:568	"Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site	555:772	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	0	56	theme	inter-domain	32:43	arg1	motions					45:51	twisting inter-domain motions	23:51	twisting inter-domain motions	23:51	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.					
33352114	4	57	theme	Parodi	556:561	arg1	"					568:568	"Parodi limit"	555:568	"Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site	555:772	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	1	58	gly	glycoprotein	150:161	arg1	glycoprotein					150:161	glycoprotein glucosyltransferase	150:181	glycoprotein glucosyltransferase (UGGT)	150:188	UDP-glucose:glycoprotein glucosyltransferase (UGGT) flags misfolded glycoproteins for ER retention.					
33352114	4	59	link	N-linked	652:659	arg1	glycan					661:666	an N-linked glycan	649:666	an N-linked glycan	649:666	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	4	60	theme	UGGT	708:711	arg1	in vitro					713:720	monomeric UGGT in vitro	698:720	monomeric UGGT in vitro	698:720	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	6	61	link	N-linked	937:944	arg1	sites					960:964	their closest N-linked glycosylation sites	923:964	their closest N-linked glycosylation sites	923:964	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	2	62	theme	thermophilum	293:304	arg1	CtUGGT					312:317	CtUGGT	312:317	CtUGGT	312:317	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	2	62	theme	thermophilum	293:304	arg1	UGGT					306:309	full-length Chaetomium thermophilum UGGT	270:309	full-length Chaetomium thermophilum UGGT (CtUGGT)	270:318	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	2	63	theme	Chaetomium	282:291	arg1	CtUGGT					312:317	CtUGGT	312:317	CtUGGT	312:317	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	2	63	theme	Chaetomium	282:291	arg1	UGGT					306:309	full-length Chaetomium thermophilum UGGT	270:309	full-length Chaetomium thermophilum UGGT (CtUGGT)	270:318	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	7	64	theme	"	1122:1122	arg1	model					1151:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	Our data support a "one-size-fits-all adjustable spanner" UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.					
33352114	2	65	theme	mutants	348:354	arg1	structures					256:265	crystal structures	248:265	crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2)	248:403	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	6	66	theme	limit	1034:1038	arg1	relevance					1010:1018	relevance	1010:1018	relevance of the Parodi limit to UGGT activity in vivo	1010:1063	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	6	67	theme	UGGT	1043:1046	arg1	activity					1048:1055	UGGT activity in vivo	1043:1063	UGGT activity in vivo	1043:1063	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	2	68	theme	full-length	270:280	arg1	CtUGGT					312:317	CtUGGT	312:317	CtUGGT	312:317	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	2	68	theme	full-length	270:280	arg1	UGGT					306:309	full-length Chaetomium thermophilum UGGT	270:309	full-length Chaetomium thermophilum UGGT (CtUGGT)	270:318	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	1	69	theme	glycoprotein	150:161	arg1	glucosyltransferase					163:181	glycoprotein glucosyltransferase	150:181	glycoprotein glucosyltransferase (UGGT)	150:188	UDP-glucose:glycoprotein glucosyltransferase (UGGT) flags misfolded glycoproteins for ER retention.					
33352114	1	69	theme	glycoprotein	150:161	arg1	UGGT					184:187	UGGT	184:187	UGGT	184:187	UDP-glucose:glycoprotein glucosyltransferase (UGGT) flags misfolded glycoproteins for ER retention.					
33352114	4	70	theme	N-linked	652:659	arg1	glycan					661:666	an N-linked glycan	649:666	an N-linked glycan	649:666	We name "Parodi limit" the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site.					
33352114	6	71	theme	Parodi	1027:1032	arg1	limit					1034:1038	the Parodi limit	1023:1038	the Parodi limit	1023:1038	Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo.					
33352114	7	72	theme	UGGT	1189:1192	arg1	domain					1200:1205	the UGGT TRXL2 domain	1185:1205	the UGGT TRXL2 domain	1185:1205	Our data support a "one-size-fits-all adjustable spanner" UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.					
33352114	0	73	dep	Clamping	0:7	arg1	glucosyltransferase					117:135	Glycoprotein glucosyltransferase	104:135	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.	0:136	Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose: Glycoprotein glucosyltransferase.					
33352114	2	74	theme	truncation	378:387	arg1	structures					256:265	crystal structures	248:265	crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2)	248:403	We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2).					
33352114	7	75	theme	UGGT	1124:1127	arg1	model					1151:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	a "one-size-fits-all adjustable spanner" UGGT substrate recognition model	1083:1155	Our data support a "one-size-fits-all adjustable spanner" UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.					
33065977	2	0	theme	MS	357:358	arg1	pathogenesis					360:371	MS pathogenesis	357:371	MS pathogenesis	357:371	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	2	1	theme	Previous	224:231	arg1	studies					233:239	Previous studies	224:239	Previous studies	224:239	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	2	2	theme	enzymatic	293:301	arg1	N-glycosylation					257:271	N-glycosylation	257:271	N-glycosylation	257:271	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	2	2	theme	enzymatic	293:301	arg1	attachment					303:312	a highly regulated enzymatic attachment	274:312	a highly regulated enzymatic attachment of complex sugars to targeted proteins	274:351	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	6	3	theme	N-glycome	1235:1243	arg1	models					1256:1261	IgG N-glycome prediction models	1231:1261	IgG N-glycome prediction models	1231:1261	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33065977	4	4	theme	IgG	833:835	arg1	traits					851:856	IgG glycosylation traits	833:856	IgG glycosylation traits	833:856	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	2	5	theme	sugars	325:330	arg1	N-glycosylation					257:271	N-glycosylation	257:271	N-glycosylation	257:271	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	2	5	theme	sugars	325:330	arg1	attachment					303:312	a highly regulated enzymatic attachment	274:312	a highly regulated enzymatic attachment of complex sugars to targeted proteins	274:351	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	5	6	theme	Significant	859:869	arg1	changes					871:877	Significant changes	859:877	Significant changes in plasma protein N-glycome composition	859:917	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	7	7	theme	increased	1359:1367	arg1	potential					1385:1393	increased proinflammatory potential	1359:1393	increased proinflammatory potential	1359:1393	Our results indicate that multiple aspects of protein glycosylation are altered in MS, showing increased proinflammatory potential.					
33065977	4	8	theme	features	651:658	arg1	levels					627:632	levels	627:632	levels of glycosylation features	627:658	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	2	9	gly	N-glycosylation	257:271	arg1	pathogenesis					360:371	MS pathogenesis	357:371	MS pathogenesis	357:371	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	5	10	theme	×	1072:1072	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	10	theme	×	1072:1072	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	7	11	theme	proinflammatory	1369:1383	arg1	potential					1385:1393	increased proinflammatory potential	1359:1393	increased proinflammatory potential	1359:1393	Our results indicate that multiple aspects of protein glycosylation are altered in MS, showing increased proinflammatory potential.					
33065977	4	12	theme	MS	668:669	arg1	cases					671:675	MS cases	668:675	MS cases	668:675	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	2	13	attach	attachment	303:312	arg2	sugars					325:330	complex sugars	317:330	complex sugars	317:330	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	2	13	attach	attachment	303:312	arg1	proteins					344:351	targeted proteins	335:351	targeted proteins	335:351	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	8	14	theme	disease	1467:1473	arg1	status					1475:1480	the disease status	1463:1480	the disease status	1463:1480	N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.					
33065977	4	15	theme	=	725:725	arg1	p					723:723	p	723:723	p = 6.96 × 10-3	723:737	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	5	16	theme	range	1049:1053	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	16	theme	range	1049:1053	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	1	17	theme	inflammatory	120:131	arg1	disorder					144:151	an inflammatory autoimmune disorder	117:151	an inflammatory autoimmune disorder affecting the central nervous system (CNS)	117:194	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	1	17	theme	inflammatory	120:131	arg1	sclerosis					99:107	Multiple sclerosis	90:107	Multiple sclerosis (MS)	90:112	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	6	18	theme	total	1194:1198	arg1	N-glycome					1207:1215	the total plasma N-glycome	1190:1215	the total plasma N-glycome	1190:1215	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33065977	5	19	theme	fucosylated	947:957	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	19	theme	fucosylated	947:957	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	20	theme	×	1060:1060	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	20	theme	×	1060:1060	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	4	21	theme	core	704:707	arg1	fucosylation					709:720	core fucosylation	704:720	core fucosylation (p = 6.96 × 10-3)	704:738	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	4	22	dep	structures	770:779	arg1	p					782:782	p	782:782	p = 1.48 × 10-2	782:796	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	5	23	theme	protein	889:895	arg1	composition					907:917	plasma protein N-glycome composition	882:917	plasma protein N-glycome composition	882:917	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	4	24	theme	high-mannose	757:768	arg1	structures					770:779	high-mannose structures	757:779	high-mannose structures (p = 1.48 × 10-2)	757:797	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	3	25	gly	N-glycosylation	414:428	arg1	proteome					450:457	the total plasma proteome	433:457	the total plasma proteome	433:457	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	3	25	gly	N-glycosylation	414:428	arg1	IgG					466:468	IgG	466:468	IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls	466:614	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	5	26	from	changes	871:877	arg1	composition					907:917	plasma protein N-glycome composition	882:917	plasma protein N-glycome composition	882:917	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	4	27	dep	fucosylation	709:720	arg1	p					723:723	p	723:723	p = 6.96 × 10-3	723:737	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	3	28	from	variation	401:409	arg1	N-glycosylation					414:428	N-glycosylation	414:428	N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls	414:614	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	1	29	with	disorder	144:151	arg1	aetiology					213:221	unresolved aetiology	202:221	unresolved aetiology	202:221	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	8	30	theme	exact	1546:1550	arg1	role					1552:1555	their exact role	1540:1555	their exact role in MS development and utility as biomarkers	1540:1599	N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.					
33065977	3	31	theme	plasma	443:448	arg1	proteome					450:457	the total plasma proteome	433:457	the total plasma proteome	433:457	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	0	32	theme	Glycosylation	0:12	arg1	Alterations					14:24	Glycosylation Alterations	0:24	Glycosylation Alterations in Multiple Sclerosis	0:46	Glycosylation Alterations in Multiple Sclerosis Show Increased Proinflammatory Potential.					
33065977	5	33	theme	antennary	937:945	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	33	theme	antennary	937:945	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	7	34	theme	protein	1310:1316	arg1	glycosylation					1318:1330	protein glycosylation	1310:1330	protein glycosylation	1310:1330	Our results indicate that multiple aspects of protein glycosylation are altered in MS, showing increased proinflammatory potential.					
33065977	8	35	theme	N-glycan	1396:1403	arg1	alterations					1405:1415	N-glycan alterations	1396:1415	N-glycan alterations showed substantial value in classification of the disease status	1396:1480	N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.					
33065977	3	36	theme	IgG	466:468	arg1	N-glycosylation					414:428	N-glycosylation	414:428	N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls	414:614	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	0	37	theme	Increased	53:61	arg1	Potential					79:87	Increased Proinflammatory Potential	53:87	Increased Proinflammatory Potential	53:87	Glycosylation Alterations in Multiple Sclerosis Show Increased Proinflammatory Potential.					
33065977	5	38	theme	p-value	1041:1047	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	38	theme	p-value	1041:1047	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	4	39	theme	=	784:784	arg1	p					782:782	p	782:782	p = 1.48 × 10-2	782:796	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	3	40	theme	plasma	482:487	arg1	protein					489:495	Both plasma protein and IgG N-glycans	477:513	protein	489:495	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	4	41	theme	glycosylation	837:849	arg1	traits					851:856	IgG glycosylation traits	833:856	IgG glycosylation traits	833:856	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	6	42	theme	Classification	1081:1094	arg1	performance					1096:1106	Classification performance	1081:1106	Classification performance of N-glycans	1081:1119	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33065977	1	43	theme	nervous	175:181	arg1	CNS					191:193	CNS	191:193	CNS	191:193	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	1	43	theme	nervous	175:181	arg1	system					183:188	the central nervous system	163:188	the central nervous system (CNS)	163:194	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	5	44	theme	plasma	882:887	arg1	composition					907:917	plasma protein N-glycome composition	882:917	plasma protein N-glycome composition	882:917	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	6	45	theme	prediction	1245:1254	arg1	models					1256:1261	IgG N-glycome prediction models	1231:1261	IgG N-glycome prediction models	1231:1261	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33065977	2	46	theme	regulated	283:291	arg1	N-glycosylation					257:271	N-glycosylation	257:271	N-glycosylation	257:271	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	2	46	theme	regulated	283:291	arg1	attachment					303:312	a highly regulated enzymatic attachment	274:312	a highly regulated enzymatic attachment of complex sugars to targeted proteins	274:351	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	6	47	theme	IgG	1231:1233	arg1	models					1256:1261	IgG N-glycome prediction models	1231:1261	IgG N-glycome prediction models	1231:1261	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33065977	4	48	dep	=	784:784	arg1	10-2					793:796	1.48 × 10-2	786:796	1.48 × 10-2	786:796	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	2	49	theme	complex	317:323	arg1	sugars					325:330	complex sugars	317:330	complex sugars	317:330	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	3	50	theme	88	583:584	arg1	age-					586:589	age-	586:589	age-	586:589	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	6	51	theme	curve	1141:1145	arg1	analysis					1147:1154	ROC curve analysis	1137:1154	ROC curve analysis	1137:1154	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33065977	4	52	theme	glycosylation	637:649	arg1	features					651:658	glycosylation features	637:658	glycosylation features	637:658	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	3	53	theme	sex-matched	595:605	arg1	controls					607:614	88 age- and sex-matched controls	583:614	88 age- and sex-matched controls	583:614	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	1	54	theme	unresolved	202:211	arg1	aetiology					213:221	unresolved aetiology	202:221	unresolved aetiology	202:221	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	8	55	theme	status	1475:1480	arg1	classification					1445:1458	classification	1445:1458	classification of the disease status	1445:1480	N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.					
33065977	3	56	theme	age-	586:589	arg1	controls					607:614	88 age- and sex-matched controls	583:614	88 age- and sex-matched controls	583:614	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	5	57	theme	N-glycome	897:905	arg1	composition					907:917	plasma protein N-glycome composition	882:917	plasma protein N-glycome composition	882:917	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	6	58	theme	plasma	1200:1205	arg1	N-glycome					1207:1215	the total plasma N-glycome	1190:1215	the total plasma N-glycome	1190:1215	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33065977	4	59	gly	fucosylation	709:720	arg1	structures					770:779	high-mannose structures	757:779	high-mannose structures (p = 1.48 × 10-2)	757:797	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	1	60	theme	autoimmune	133:142	arg1	disorder					144:151	an inflammatory autoimmune disorder	117:151	an inflammatory autoimmune disorder affecting the central nervous system (CNS)	117:194	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	1	60	theme	autoimmune	133:142	arg1	sclerosis					99:107	Multiple sclerosis	90:107	Multiple sclerosis (MS)	90:112	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	3	61	theme	MS	570:571	arg1	cases					573:577	83 MS cases	567:577	83 MS cases	567:577	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	8	62	theme	additional	1496:1505	arg1	studies					1507:1513	additional studies	1496:1513	additional studies	1496:1513	N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.					
33065977	5	63	theme	10-2-4.28	1062:1070	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	63	theme	10-2-4.28	1062:1070	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	6	64	theme	0.852	1180:1184	arg1	AUC					1173:1175	an AUC	1170:1175	an AUC of 0.852 for the total plasma N-glycome	1170:1215	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33065977	6	64	theme	0.852	1180:1184	arg1	0.798					1221:1225	0.798	1221:1225	0.798 for IgG N-glycome prediction models	1221:1261	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33065977	1	65	theme	central	167:173	arg1	CNS					191:193	CNS	191:193	CNS	191:193	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	1	65	theme	central	167:173	arg1	system					183:188	the central nervous system	163:188	the central nervous system (CNS)	163:194	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	5	66	gly	tetrasialylated	969:983	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	66	gly	tetrasialylated	969:983	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	0	67	from	Alterations	14:24	arg1	Sclerosis					38:46	Multiple Sclerosis	29:46	Multiple Sclerosis	29:46	Glycosylation Alterations in Multiple Sclerosis Show Increased Proinflammatory Potential.					
33065977	5	68	theme	tri-	960:963	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	68	theme	tri-	960:963	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	1	69	theme	Multiple	90:97	arg1	MS					110:111	MS	110:111	MS	110:111	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	1	69	theme	Multiple	90:97	arg1	sclerosis					99:107	Multiple sclerosis	90:107	Multiple sclerosis (MS)	90:112	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	1	69	theme	Multiple	90:97	arg1	disorder					144:151	an inflammatory autoimmune disorder	117:151	an inflammatory autoimmune disorder affecting the central nervous system (CNS)	117:194	Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology.					
33065977	7	70	theme	glycosylation	1318:1330	arg1	aspects					1299:1305	multiple aspects	1290:1305	multiple aspects of protein glycosylation	1290:1330	Our results indicate that multiple aspects of protein glycosylation are altered in MS, showing increased proinflammatory potential.					
33065977	5	71	gly	fucosylated	947:957	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	71	gly	fucosylated	947:957	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	4	72	theme	structures	770:779	arg1	fucosylation					709:720	core fucosylation	704:720	core fucosylation (p = 6.96 × 10-3)	704:738	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	4	72	theme	structures	770:779	arg1	abundance					744:752	abundance	744:752	abundance of high-mannose structures (p = 1.48 × 10-2)	744:797	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	8	73	theme	MS	1560:1561	arg1	development					1563:1573	MS development	1560:1573	MS development	1560:1573	N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.					
33065977	3	74	theme	proteome	450:457	arg1	N-glycosylation					414:428	N-glycosylation	414:428	N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls	414:614	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	0	75	theme	Multiple	29:36	arg1	Sclerosis					38:46	Multiple Sclerosis	29:46	Multiple Sclerosis	29:46	Glycosylation Alterations in Multiple Sclerosis Show Increased Proinflammatory Potential.					
33065977	4	76	dep	=	725:725	arg1	×					732:732	6.96 × 10-3	727:737	6.96 × 10-3	727:737	Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 × 10-3) and abundance of high-mannose structures (p = 1.48 × 10-2) were the most prominently altered IgG glycosylation traits.					
33065977	7	77	theme	multiple	1290:1297	arg1	aspects					1299:1305	multiple aspects	1290:1305	multiple aspects of protein glycosylation	1290:1330	Our results indicate that multiple aspects of protein glycosylation are altered in MS, showing increased proinflammatory potential.					
33065977	3	78	theme	individual	390:399	arg1	variation					401:409	individual variation	390:409	individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls	390:614	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	5	79	theme	tetrasialylated	969:983	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	79	theme	tetrasialylated	969:983	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	8	80	from	role	1552:1555	arg1	development					1563:1573	MS development	1560:1573	MS development	1560:1573	N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.					
33065977	8	80	from	role	1552:1555	arg1	utility					1579:1585	utility	1579:1585	utility	1579:1585	N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.					
33065977	5	81	theme	high-branched	1016:1028	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	81	theme	high-branched	1016:1028	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	3	82	theme	total	437:441	arg1	proteome					450:457	the total plasma proteome	433:457	the total plasma proteome	433:457	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	0	83	theme	Proinflammatory	63:77	arg1	Potential					79:87	Increased Proinflammatory Potential	53:87	Increased Proinflammatory Potential	53:87	Glycosylation Alterations in Multiple Sclerosis Show Increased Proinflammatory Potential.					
33065977	6	84	theme	ROC	1137:1139	arg1	analysis					1147:1154	ROC curve analysis	1137:1154	ROC curve analysis	1137:1154	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33065977	3	85	theme	IgG	501:503	arg1	N-glycans					505:513	Both plasma protein and IgG N-glycans	477:513	N-glycans	505:513	We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls.					
33065977	5	86	theme	tri-	986:989	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	86	theme	tri-	986:989	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	8	87	theme	substantial	1424:1434	arg1	value					1436:1440	substantial value	1424:1440	substantial value	1424:1440	N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.					
33065977	2	88	theme	targeted	335:342	arg1	proteins					344:351	targeted proteins	335:351	targeted proteins	335:351	Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis.					
33065977	5	89	theme	tetragalactosylated	995:1013	arg1	N-glycans					1030:1038	high-branched N-glycans	1016:1038	high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2)	1016:1078	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	5	89	theme	tetragalactosylated	995:1013	arg1	10-2					1074:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	p-value range 1.66 × 10-2-4.28 × 10-2	1041:1077	Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 × 10-2-4.28 × 10-2).					
33065977	6	90	theme	N-glycans	1111:1119	arg1	performance					1096:1106	Classification performance	1081:1106	Classification performance of N-glycans	1081:1119	Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models.					
33093110	2	0	gly	O-glycosylated	281:294	arg1	entities					296:303	two distinct O-glycosylated entities	268:303	two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form	268:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	0	gly	O-glycosylated	281:294	arg1	form					322:325	a major form	314:325	a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota,	314:421	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	0	gly	O-glycosylated	281:294	arg1	form					435:438	a minor form	427:438	a minor form derived from the distal colon, which adheres to the major form	427:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	4	1	dep	structure	674:682	arg1	the					670:672	the	670:672	the	670:672	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	4	2	from	transcription	726:738	arg1	mucosa					753:758	the colon mucosa	743:758	the colon mucosa	743:758	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	4	3	theme	colon-derived	642:654	arg1	Muc2					656:659	proximal colon-derived Muc2	633:659	proximal colon-derived Muc2	633:659	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	3	4	theme	proximal	582:589	arg1	cells					604:608	proximal colon goblet cells	582:608	proximal colon goblet cells	582:608	The microbiota directs its own encapsulation by inducing Muc2 production from proximal colon goblet cells.					
33093110	2	5	attach	derived	440:446	arg2	entities					296:303	two distinct O-glycosylated entities	268:303	two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form	268:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	5	attach	derived	440:446	arg2	form					435:438	a minor form	427:438	a minor form derived from the distal colon, which adheres to the major form	427:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	5	attach	derived	440:446	arg1	colon					464:468	the distal colon	453:468	the distal colon	453:468	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	6	theme	distinct	272:279	arg1	entities					296:303	two distinct O-glycosylated entities	268:303	two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form	268:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	6	theme	distinct	272:279	arg1	form					322:325	a major form	314:325	a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota,	314:421	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	6	theme	distinct	272:279	arg1	form					435:438	a minor form	427:438	a minor form derived from the distal colon, which adheres to the major form	427:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	5	7	theme	symbiosis	884:892	arg1	regulation					854:863	the regulation	850:863	the regulation of host-microbiota symbiosis	850:892	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	0	8	link	colon-derived	9:21	arg1	mucus					38:42	Proximal colon-derived O-glycosylated mucus	0:42	Proximal colon-derived O-glycosylated mucus	0:42	Proximal colon-derived O-glycosylated mucus encapsulates and modulates the microbiota.					
33093110	4	9	from	O-glycans	620:628	arg1	Muc2					656:659	proximal colon-derived Muc2	633:659	proximal colon-derived Muc2	633:659	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	5	10	theme	proximal	780:787	arg1	element					839:845	an important element	826:845	an important element in the regulation of host-microbiota symbiosis	826:892	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	5	10	theme	proximal	780:787	arg1	control					795:801	proximal colon control	780:801	proximal colon control of mucin production	780:821	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	1	11	theme	Colon	87:91	arg1	mucus					93:97	Colon mucus	87:97	Colon mucus	87:97	Colon mucus segregates the intestinal microbiota from host tissues, but how it organizes to function throughout the colon is unclear.					
33093110	3	12	theme	own	531:533	arg1	encapsulation					535:547	its own encapsulation	527:547	its own encapsulation	527:547	The microbiota directs its own encapsulation by inducing Muc2 production from proximal colon goblet cells.					
33093110	1	13	from	tissues	146:152	arg1	microbiota					125:134	the intestinal microbiota	110:134	the intestinal microbiota from host tissues	110:152	Colon mucus segregates the intestinal microbiota from host tissues, but how it organizes to function throughout the colon is unclear.					
33093110	5	14	theme	mucin	806:810	arg1	production					812:821	mucin production	806:821	mucin production	806:821	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	0	15	theme	colon-derived	9:21	arg1	mucus					38:42	Proximal colon-derived O-glycosylated mucus	0:42	Proximal colon-derived O-glycosylated mucus	0:42	Proximal colon-derived O-glycosylated mucus encapsulates and modulates the microbiota.					
33093110	2	16	theme	Muc2	308:311	arg1	entities					296:303	two distinct O-glycosylated entities	268:303	two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form	268:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	16	theme	Muc2	308:311	arg1	form					322:325	a major form	314:325	a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota,	314:421	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	16	theme	Muc2	308:311	arg1	form					435:438	a minor form	427:438	a minor form derived from the distal colon, which adheres to the major form	427:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	5	17	theme	production	812:821	arg1	element					839:845	an important element	826:845	an important element in the regulation of host-microbiota symbiosis	826:892	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	5	17	theme	production	812:821	arg1	control					795:801	proximal colon control	780:801	proximal colon control of mucin production	780:821	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	0	18	theme	Proximal	0:7	arg1	mucus					38:42	Proximal colon-derived O-glycosylated mucus	0:42	Proximal colon-derived O-glycosylated mucus	0:42	Proximal colon-derived O-glycosylated mucus encapsulates and modulates the microbiota.					
33093110	4	19	link	colon-derived	642:654	arg1	Muc2					656:659	proximal colon-derived Muc2	633:659	proximal colon-derived Muc2	633:659	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	4	20	from	function	688:695	arg1	mucosa					753:758	the colon mucosa	743:758	the colon mucosa	743:758	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	1	21	theme	intestinal	114:123	arg1	microbiota					125:134	the intestinal microbiota	110:134	the intestinal microbiota from host tissues	110:152	Colon mucus segregates the intestinal microbiota from host tissues, but how it organizes to function throughout the colon is unclear.					
33093110	5	22	from	element	839:845	arg1	regulation					854:863	the regulation	850:863	the regulation of host-microbiota symbiosis	850:892	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	0	23	theme	O-glycosylated	23:36	arg1	mucus					38:42	Proximal colon-derived O-glycosylated mucus	0:42	Proximal colon-derived O-glycosylated mucus	0:42	Proximal colon-derived O-glycosylated mucus encapsulates and modulates the microbiota.					
33093110	2	24	theme	O-glycosylated	281:294	arg1	entities					296:303	two distinct O-glycosylated entities	268:303	two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form	268:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	24	theme	O-glycosylated	281:294	arg1	form					322:325	a major form	314:325	a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota,	314:421	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	24	theme	O-glycosylated	281:294	arg1	form					435:438	a minor form	427:438	a minor form derived from the distal colon, which adheres to the major form	427:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	4	25	theme	proximal	633:640	arg1	Muc2					656:659	proximal colon-derived Muc2	633:659	proximal colon-derived Muc2	633:659	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	2	26	theme	major	316:320	arg1	entities					296:303	two distinct O-glycosylated entities	268:303	two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form	268:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	26	theme	major	316:320	arg1	form					322:325	a major form	314:325	a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota,	314:421	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	27	theme	major	492:496	arg1	form					498:501	the major form	488:501	the major form	488:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	5	28	theme	colon	789:793	arg1	element					839:845	an important element	826:845	an important element in the regulation of host-microbiota symbiosis	826:892	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	5	28	theme	colon	789:793	arg1	control					795:801	proximal colon control	780:801	proximal colon control of mucin production	780:821	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	2	29	theme	fecal	382:386	arg1	material					388:395	the fecal material	378:395	the fecal material including the microbiota	378:420	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	3	30	theme	colon	591:595	arg1	cells					604:608	proximal colon goblet cells	582:608	proximal colon goblet cells	582:608	The microbiota directs its own encapsulation by inducing Muc2 production from proximal colon goblet cells.					
33093110	2	31	theme	minor	429:433	arg1	entities					296:303	two distinct O-glycosylated entities	268:303	two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form	268:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	31	theme	minor	429:433	arg1	form					435:438	a minor form	427:438	a minor form derived from the distal colon, which adheres to the major form	427:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	3	32	theme	goblet	597:602	arg1	cells					604:608	proximal colon goblet cells	582:608	proximal colon goblet cells	582:608	The microbiota directs its own encapsulation by inducing Muc2 production from proximal colon goblet cells.					
33093110	0	33	gly	O-glycosylated	23:36	arg1	mucus					38:42	Proximal colon-derived O-glycosylated mucus	0:42	Proximal colon-derived O-glycosylated mucus	0:42	Proximal colon-derived O-glycosylated mucus encapsulates and modulates the microbiota.					
33093110	2	34	dep	entities	296:303	arg1	entities					296:303	two distinct O-glycosylated entities	268:303	two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form	268:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	34	dep	entities	296:303	arg1	form					322:325	a major form	314:325	a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota,	314:421	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	2	34	dep	entities	296:303	arg1	form					435:438	a minor form	427:438	a minor form derived from the distal colon, which adheres to the major form	427:501	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	1	35	theme	host	141:144	arg1	tissues					146:152	host tissues	141:152	host tissues	141:152	Colon mucus segregates the intestinal microbiota from host tissues, but how it organizes to function throughout the colon is unclear.					
33093110	4	36	theme	microbiota	704:713	arg1	function					688:695	function	688:695	function	688:695	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	4	36	theme	microbiota	704:713	arg1	structure					674:682	structure	674:682	structure	674:682	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	4	36	theme	microbiota	704:713	arg1	transcription					726:738	transcription	726:738	the structure and function of the microbiota as well as transcription in the colon mucosa	670:758	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	2	37	theme	colon	244:248	arg1	mucus					250:254	colon mucus	244:254	colon mucus	244:254	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	5	38	theme	host-microbiota	868:882	arg1	symbiosis					884:892	host-microbiota symbiosis	868:892	host-microbiota symbiosis	868:892	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	3	39	theme	Muc2	561:564	arg1	production					566:575	Muc2 production	561:575	Muc2 production	561:575	The microbiota directs its own encapsulation by inducing Muc2 production from proximal colon goblet cells.					
33093110	2	40	theme	proximal	343:350	arg1	colon					352:356	the proximal colon	339:356	the proximal colon	339:356	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	4	41	from	structure	674:682	arg1	mucosa					753:758	the colon mucosa	743:758	the colon mucosa	743:758	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	2	42	theme	distal	457:462	arg1	colon					464:468	the distal colon	453:468	the distal colon	453:468	In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: a major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form.					
33093110	4	43	theme	colon	747:751	arg1	mucosa					753:758	the colon mucosa	743:758	the colon mucosa	743:758	In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa.					
33093110	5	44	theme	important	829:837	arg1	element					839:845	an important element	826:845	an important element in the regulation of host-microbiota symbiosis	826:892	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
33093110	5	44	theme	important	829:837	arg1	control					795:801	proximal colon control	780:801	proximal colon control of mucin production	780:821	Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.					
34767945	11	0	theme	PGD	1891:1893	arg1	development					1895:1905	PGD development	1891:1905	PGD development	1891:1905	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	12	1	theme	PGD	2012:2014	arg1	development					2016:2026	PGD development	2012:2026	PGD development	2012:2026	Taken together, these data represent a potential novel method for identifying COPD patients at risk for PGD development and may provide clues to mechanisms by which antibody N-glycan signatures could contribute to antibody-mediated PGD pathogenesis.					
34767945	9	2	theme	IgG1	1523:1526	arg1	recipients					1565:1574	compared PGD- recipients	1551:1574	compared PGD- recipients	1551:1574	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	9	2	theme	IgG1	1523:1526	arg1	signatures					1537:1546	significantly increased IgG1 N-glycan signatures	1499:1546	significantly increased IgG1 N-glycan signatures	1499:1546	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	2	3	theme	autoimmune	268:277	arg1	changes					279:285	autoimmune changes	268:285	autoimmune changes	268:285	The pathogenesis of COPD is complex; however, recent studies suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status.					
34767945	5	4	theme	circulating	845:855	arg1	elastin					857:863	total immunoglobulin and circulating elastin and collagen autoantibody levels	820:896	elastin	857:863	We measured total immunoglobulin and circulating elastin and collagen autoantibody levels in a cohort of COPD lung transplant recipients pre- and post-LTx.					
34767945	6	5	theme	PGD-	1097:1100	arg1	recipients					1102:1111	PGD+ and PGD- recipients	1088:1111	PGD+ and PGD- recipients	1088:1111	No significant differences were seen in total, elastin, or collagen IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies between PGD+ and PGD- recipients.					
34767945	4	6	theme	primary	645:651	arg1	PGD					672:674	PGD	672:674	PGD	672:674	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	4	6	theme	primary	645:651	arg1	dysfunction					659:669	primary graft dysfunction	645:669	primary graft dysfunction (PGD)	645:675	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	11	7	theme	significant	1808:1818	arg1	increases					1820:1828	multiple significant increases	1799:1828	multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development	1799:1905	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	3	8	theme	COPD-related	535:546	arg1	autoantibodies					548:561	COPD-related autoantibodies	535:561	COPD-related autoantibodies	535:561	COPD patients make up approximately 30% of all lung transplants (LTx) annually, however, little is known regarding the relationship between COPD-related autoantibodies and LTx outcomes.					
34767945	1	9	theme	death	182:186	arg1	worldwide					188:196	death worldwide	182:196	death worldwide	182:196	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	6	10	theme	PGD+	1088:1091	arg1	recipients					1102:1111	PGD+ and PGD- recipients	1088:1111	PGD+ and PGD- recipients	1088:1111	No significant differences were seen in total, elastin, or collagen IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies between PGD+ and PGD- recipients.					
34767945	12	11	theme	antibody-mediated	2122:2138	arg1	pathogenesis					2144:2155	antibody-mediated PGD pathogenesis	2122:2155	antibody-mediated PGD pathogenesis	2122:2155	Taken together, these data represent a potential novel method for identifying COPD patients at risk for PGD development and may provide clues to mechanisms by which antibody N-glycan signatures could contribute to antibody-mediated PGD pathogenesis.					
34767945	9	12	theme	COPD-LTx	1457:1464	arg1	recipients					1466:1475	COPD-LTx recipients	1457:1475	COPD-LTx recipients who developed PGD	1457:1493	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	8	13	theme	IgG-specific	1414:1425	arg1	structures					1445:1454	IgG-specific antibody N-glycan structures	1414:1454	IgG-specific antibody N-glycan structures	1414:1454	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	2	14	dep	complex	227:233	arg1	complex					227:233	complex	227:233	complex	227:233	The pathogenesis of COPD is complex; however, recent studies suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status.					
34767945	2	14	dep	complex	227:233	arg1	suggest					260:266	suggest	260:266	suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status	260:392	The pathogenesis of COPD is complex; however, recent studies suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status.					
34767945	2	14	dep	complex	227:233	arg1	pathogenesis					203:214	The pathogenesis	199:214	The pathogenesis of COPD	199:222	The pathogenesis of COPD is complex; however, recent studies suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status.					
34767945	10	15	theme	antibody	1637:1644	arg1	levels					1646:1651	immunoglobulin and autoreactive antibody levels	1605:1651	immunoglobulin and autoreactive antibody levels	1605:1651	In conclusion, we show that immunoglobulin and autoreactive antibody levels are not significantly different in COPD LTx recipients that develop PGD.					
34767945	11	16	theme	specific	1838:1845	arg1	signatures					1856:1865	IgG1 specific N-glycan signatures	1833:1865	IgG1 specific N-glycan signatures that were predictive of PGD development	1833:1905	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	8	17	theme	N-glycan	1436:1443	arg1	structures					1445:1454	IgG-specific antibody N-glycan structures	1414:1454	IgG-specific antibody N-glycan structures	1414:1454	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	6	18	theme	IgG3	1054:1057	arg1	antibodies					1069:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	No significant differences were seen in total, elastin, or collagen IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies between PGD+ and PGD- recipients.					
34767945	4	19	theme	circulating	690:700	arg1	levels					715:720	altered circulating autoantibody levels	682:720	altered circulating autoantibody levels	682:720	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	5	20	theme	recipients	934:943	arg1	cohort					903:908	a cohort	901:908	a cohort of COPD lung transplant recipients pre- and post-LTx	901:961	We measured total immunoglobulin and circulating elastin and collagen autoantibody levels in a cohort of COPD lung transplant recipients pre- and post-LTx.					
34767945	11	21	theme	glycomic	1753:1760	arg1	analysis					1762:1769	a novel IgG glycomic analysis	1741:1769	a novel IgG glycomic analysis	1741:1769	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	11	22	dep	able	1779:1782	arg1	using					1735:1739	using	1735:1739	using a novel IgG glycomic analysis	1735:1769	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	5	23	theme	COPD	913:916	arg1	recipients					934:943	COPD lung transplant recipients	913:943	COPD lung transplant recipients pre- and post-LTx	913:961	We measured total immunoglobulin and circulating elastin and collagen autoantibody levels in a cohort of COPD lung transplant recipients pre- and post-LTx.					
34767945	7	24	theme	IgG	1247:1249	arg1	profiles					1265:1272	altered IgG glycosylation profiles	1239:1272	altered IgG glycosylation profiles	1239:1272	Antibody function can be greatly altered by glycosylation changes to the antibody Fc region and recent studies have reported altered IgG glycosylation profiles in COPD patients.					
34767945	11	25	theme	novel	1743:1747	arg1	analysis					1762:1769	a novel IgG glycomic analysis	1741:1769	a novel IgG glycomic analysis	1741:1769	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	0	26	theme	dysfunction	71:81	arg1	onset					83:87	primary graft dysfunction onset	57:87	primary graft dysfunction onset in COPD patients	57:104	Pro-inflammatory IgG1 N-glycan signature correlates with primary graft dysfunction onset in COPD patients.					
34767945	5	27	theme	autoantibody	878:889	arg1	levels					891:896	total immunoglobulin and circulating elastin and collagen autoantibody levels	820:896	levels	891:896	We measured total immunoglobulin and circulating elastin and collagen autoantibody levels in a cohort of COPD lung transplant recipients pre- and post-LTx.					
34767945	12	28	theme	antibody	2073:2080	arg1	signatures					2091:2100	antibody N-glycan signatures	2073:2100	antibody N-glycan signatures	2073:2100	Taken together, these data represent a potential novel method for identifying COPD patients at risk for PGD development and may provide clues to mechanisms by which antibody N-glycan signatures could contribute to antibody-mediated PGD pathogenesis.					
34767945	7	29	theme	glycosylation	1158:1170	arg1	changes					1172:1178	glycosylation changes	1158:1178	glycosylation changes	1158:1178	Antibody function can be greatly altered by glycosylation changes to the antibody Fc region and recent studies have reported altered IgG glycosylation profiles in COPD patients.					
34767945	1	30	theme	obstructive	115:125	arg1	COPD					146:149	COPD	146:149	COPD	146:149	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	1	30	theme	obstructive	115:125	arg1	disease					137:143	Chronic obstructive pulmonary disease	107:143	Chronic obstructive pulmonary disease (COPD)	107:150	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	1	30	theme	obstructive	115:125	arg1	cause					173:177	the third leading cause	155:177	the third leading cause of death worldwide	155:196	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	5	31	theme	post-LTx	954:961	arg1	recipients					934:943	COPD lung transplant recipients	913:943	COPD lung transplant recipients pre- and post-LTx	913:961	We measured total immunoglobulin and circulating elastin and collagen autoantibody levels in a cohort of COPD lung transplant recipients pre- and post-LTx.					
34767945	6	32	theme	significant	967:977	arg1	differences					979:989	No significant differences	964:989	No significant differences	964:989	No significant differences were seen in total, elastin, or collagen IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies between PGD+ and PGD- recipients.					
34767945	3	33	theme	lung	442:445	arg1	LTx					460:462	LTx	460:462	LTx	460:462	COPD patients make up approximately 30% of all lung transplants (LTx) annually, however, little is known regarding the relationship between COPD-related autoantibodies and LTx outcomes.					
34767945	3	33	theme	lung	442:445	arg1	transplants					447:457	all lung transplants	438:457	all lung transplants (LTx)	438:463	COPD patients make up approximately 30% of all lung transplants (LTx) annually, however, little is known regarding the relationship between COPD-related autoantibodies and LTx outcomes.					
34767945	2	34	theme	disease	379:385	arg1	status					387:392	disease status	379:392	disease status	379:392	The pathogenesis of COPD is complex; however, recent studies suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status.					
34767945	8	35	theme	novel	1316:1320	arg1	approach					1381:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	0	36	theme	Pro-inflammatory	0:15	arg1	signature					31:39	Pro-inflammatory IgG1 N-glycan signature	0:39	Pro-inflammatory IgG1 N-glycan signature	0:39	Pro-inflammatory IgG1 N-glycan signature correlates with primary graft dysfunction onset in COPD patients.					
34767945	5	37	theme	transplant	923:932	arg1	recipients					934:943	COPD lung transplant recipients	913:943	COPD lung transplant recipients pre- and post-LTx	913:961	We measured total immunoglobulin and circulating elastin and collagen autoantibody levels in a cohort of COPD lung transplant recipients pre- and post-LTx.					
34767945	4	38	theme	phenotypic	726:735	arg1	changes					737:743	phenotypic changes	726:743	phenotypic changes	726:743	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	0	39	theme	N-glycan	22:29	arg1	signature					31:39	Pro-inflammatory IgG1 N-glycan signature	0:39	Pro-inflammatory IgG1 N-glycan signature	0:39	Pro-inflammatory IgG1 N-glycan signature correlates with primary graft dysfunction onset in COPD patients.					
34767945	5	40	theme	pre-	945:948	arg1	recipients					934:943	COPD lung transplant recipients	913:943	COPD lung transplant recipients pre- and post-LTx	913:961	We measured total immunoglobulin and circulating elastin and collagen autoantibody levels in a cohort of COPD lung transplant recipients pre- and post-LTx.					
34767945	7	41	theme	glycosylation	1251:1263	arg1	profiles					1265:1272	altered IgG glycosylation profiles	1239:1272	altered IgG glycosylation profiles	1239:1272	Antibody function can be greatly altered by glycosylation changes to the antibody Fc region and recent studies have reported altered IgG glycosylation profiles in COPD patients.					
34767945	3	42	theme	COPD	395:398	arg1	patients					400:407	COPD patients	395:407	COPD patients	395:407	COPD patients make up approximately 30% of all lung transplants (LTx) annually, however, little is known regarding the relationship between COPD-related autoantibodies and LTx outcomes.					
34767945	8	43	theme	N-glycoprotein	1358:1371	arg1	approach					1381:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	10	44	theme	COPD	1688:1691	arg1	recipients					1697:1706	COPD LTx recipients	1688:1706	COPD LTx recipients that develop PGD	1688:1723	In conclusion, we show that immunoglobulin and autoreactive antibody levels are not significantly different in COPD LTx recipients that develop PGD.					
34767945	7	45	theme	altered	1239:1245	arg1	profiles					1265:1272	altered IgG glycosylation profiles	1239:1272	altered IgG glycosylation profiles	1239:1272	Antibody function can be greatly altered by glycosylation changes to the antibody Fc region and recent studies have reported altered IgG glycosylation profiles in COPD patients.					
34767945	5	46	theme	collagen	869:876	arg1	levels					891:896	total immunoglobulin and circulating elastin and collagen autoantibody levels	820:896	levels	891:896	We measured total immunoglobulin and circulating elastin and collagen autoantibody levels in a cohort of COPD lung transplant recipients pre- and post-LTx.					
34767945	0	47	theme	primary	57:63	arg1	onset					83:87	primary graft dysfunction onset	57:87	primary graft dysfunction onset in COPD patients	57:104	Pro-inflammatory IgG1 N-glycan signature correlates with primary graft dysfunction onset in COPD patients.					
34767945	10	48	from	different	1675:1683	arg1	recipients					1697:1706	COPD LTx recipients	1688:1706	COPD LTx recipients that develop PGD	1688:1723	In conclusion, we show that immunoglobulin and autoreactive antibody levels are not significantly different in COPD LTx recipients that develop PGD.					
34767945	6	49	theme	IgG4	1064:1067	arg1	antibodies					1069:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	No significant differences were seen in total, elastin, or collagen IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies between PGD+ and PGD- recipients.					
34767945	3	50	theme	transplants	447:457	arg1	LTx					460:462	LTx	460:462	LTx	460:462	COPD patients make up approximately 30% of all lung transplants (LTx) annually, however, little is known regarding the relationship between COPD-related autoantibodies and LTx outcomes.					
34767945	3	50	theme	transplants	447:457	arg1	transplants					447:457	all lung transplants	438:457	all lung transplants (LTx)	438:463	COPD patients make up approximately 30% of all lung transplants (LTx) annually, however, little is known regarding the relationship between COPD-related autoantibodies and LTx outcomes.					
34767945	3	50	theme	transplants	447:457	arg1	%					433:433	approximately 30%	417:433	approximately 30% of all lung transplants (LTx)	417:463	COPD patients make up approximately 30% of all lung transplants (LTx) annually, however, little is known regarding the relationship between COPD-related autoantibodies and LTx outcomes.					
34767945	7	51	theme	recent	1210:1215	arg1	studies					1217:1223	recent studies	1210:1223	recent studies	1210:1223	Antibody function can be greatly altered by glycosylation changes to the antibody Fc region and recent studies have reported altered IgG glycosylation profiles in COPD patients.					
34767945	8	52	theme	spectrometry-based	1327:1344	arg1	approach					1381:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	11	53	theme	development	1895:1905	arg1	predictive					1877:1886	predictive	1877:1886	predictive	1877:1886	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	10	54	from	recipients	1697:1706	arg1	different					1675:1683	different	1675:1683	different	1675:1683	In conclusion, we show that immunoglobulin and autoreactive antibody levels are not significantly different in COPD LTx recipients that develop PGD.					
34767945	7	55	theme	antibody	1187:1194	arg1	region					1199:1204	the antibody Fc region	1183:1204	the antibody Fc region	1183:1204	Antibody function can be greatly altered by glycosylation changes to the antibody Fc region and recent studies have reported altered IgG glycosylation profiles in COPD patients.					
34767945	1	56	theme	leading	165:171	arg1	cause					173:177	the third leading cause	155:177	the third leading cause of death worldwide	155:196	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	1	56	theme	leading	165:171	arg1	disease					137:143	Chronic obstructive pulmonary disease	107:143	Chronic obstructive pulmonary disease (COPD)	107:150	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	10	57	theme	immunoglobulin	1605:1618	arg1	levels					1646:1651	immunoglobulin and autoreactive antibody levels	1605:1651	immunoglobulin and autoreactive antibody levels	1605:1651	In conclusion, we show that immunoglobulin and autoreactive antibody levels are not significantly different in COPD LTx recipients that develop PGD.					
34767945	4	58	contain	have	677:680	arg1	patients					607:614	COPD patients	602:614	COPD patients that undergo LTx and develop primary graft dysfunction (PGD)	602:675	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	4	58	contain	have	677:680	arg2	levels					715:720	altered circulating autoantibody levels	682:720	altered circulating autoantibody levels	682:720	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	4	58	contain	have	677:680	arg2	changes					737:743	phenotypic changes	726:743	phenotypic changes	726:743	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	12	59	theme	COPD	1986:1989	arg1	patients					1991:1998	COPD patients	1986:1998	COPD patients	1986:1998	Taken together, these data represent a potential novel method for identifying COPD patients at risk for PGD development and may provide clues to mechanisms by which antibody N-glycan signatures could contribute to antibody-mediated PGD pathogenesis.					
34767945	11	60	from	increases	1820:1828	arg1	signatures					1856:1865	IgG1 specific N-glycan signatures	1833:1865	IgG1 specific N-glycan signatures that were predictive of PGD development	1833:1905	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	8	61	from	changes	1403:1409	arg1	structures					1445:1454	IgG-specific antibody N-glycan structures	1414:1454	IgG-specific antibody N-glycan structures	1414:1454	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	1	62	theme	worldwide	188:196	arg1	cause					173:177	the third leading cause	155:177	the third leading cause of death worldwide	155:196	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	1	62	theme	worldwide	188:196	arg1	disease					137:143	Chronic obstructive pulmonary disease	107:143	Chronic obstructive pulmonary disease (COPD)	107:150	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	8	63	theme	antibody	1427:1434	arg1	structures					1445:1454	IgG-specific antibody N-glycan structures	1414:1454	IgG-specific antibody N-glycan structures	1414:1454	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	4	64	theme	COPD	602:605	arg1	patients					607:614	COPD patients	602:614	COPD patients that undergo LTx and develop primary graft dysfunction (PGD)	602:675	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	11	65	theme	multiple	1799:1806	arg1	increases					1820:1828	multiple significant increases	1799:1828	multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development	1799:1905	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	3	66	theme	LTx	567:569	arg1	outcomes					571:578	LTx outcomes	567:578	LTx outcomes	567:578	COPD patients make up approximately 30% of all lung transplants (LTx) annually, however, little is known regarding the relationship between COPD-related autoantibodies and LTx outcomes.					
34767945	5	67	theme	total	820:824	arg1	immunoglobulin					826:839	total immunoglobulin and circulating elastin and collagen autoantibody levels	820:896	immunoglobulin	826:839	We measured total immunoglobulin and circulating elastin and collagen autoantibody levels in a cohort of COPD lung transplant recipients pre- and post-LTx.					
34767945	12	68	theme	novel	1957:1961	arg1	method					1963:1968	a potential novel method	1945:1968	a potential novel method for identifying COPD patients at risk for PGD development and may provide clues to mechanisms by which antibody N-glycan signatures could contribute to antibody-mediated PGD pathogenesis	1945:2155	Taken together, these data represent a potential novel method for identifying COPD patients at risk for PGD development and may provide clues to mechanisms by which antibody N-glycan signatures could contribute to antibody-mediated PGD pathogenesis.					
34767945	0	69	from	onset	83:87	arg1	patients					97:104	COPD patients	92:104	COPD patients	92:104	Pro-inflammatory IgG1 N-glycan signature correlates with primary graft dysfunction onset in COPD patients.					
34767945	12	70	theme	PGD	2140:2142	arg1	pathogenesis					2144:2155	antibody-mediated PGD pathogenesis	2122:2155	antibody-mediated PGD pathogenesis	2122:2155	Taken together, these data represent a potential novel method for identifying COPD patients at risk for PGD development and may provide clues to mechanisms by which antibody N-glycan signatures could contribute to antibody-mediated PGD pathogenesis.					
34767945	10	71	theme	autoreactive	1624:1635	arg1	antibody					1637:1644	autoreactive antibody	1624:1644	autoreactive antibody	1624:1644	In conclusion, we show that immunoglobulin and autoreactive antibody levels are not significantly different in COPD LTx recipients that develop PGD.					
34767945	11	72	theme	N-glycan	1847:1854	arg1	signatures					1856:1865	IgG1 specific N-glycan signatures	1833:1865	IgG1 specific N-glycan signatures that were predictive of PGD development	1833:1905	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	2	73	theme	COPD	219:222	arg1	complex					227:233	complex	227:233	complex	227:233	The pathogenesis of COPD is complex; however, recent studies suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status.					
34767945	2	73	theme	COPD	219:222	arg1	suggest					260:266	suggest	260:266	suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status	260:392	The pathogenesis of COPD is complex; however, recent studies suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status.					
34767945	2	73	theme	COPD	219:222	arg1	pathogenesis					203:214	The pathogenesis	199:214	The pathogenesis of COPD	199:222	The pathogenesis of COPD is complex; however, recent studies suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status.					
34767945	1	74	theme	pulmonary	127:135	arg1	COPD					146:149	COPD	146:149	COPD	146:149	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	1	74	theme	pulmonary	127:135	arg1	disease					137:143	Chronic obstructive pulmonary disease	107:143	Chronic obstructive pulmonary disease (COPD)	107:150	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	1	74	theme	pulmonary	127:135	arg1	cause					173:177	the third leading cause	155:177	the third leading cause of death worldwide	155:196	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	12	75	theme	N-glycan	2082:2089	arg1	signatures					2091:2100	antibody N-glycan signatures	2073:2100	antibody N-glycan signatures	2073:2100	Taken together, these data represent a potential novel method for identifying COPD patients at risk for PGD development and may provide clues to mechanisms by which antibody N-glycan signatures could contribute to antibody-mediated PGD pathogenesis.					
34767945	11	76	theme	IgG1	1833:1836	arg1	signatures					1856:1865	IgG1 specific N-glycan signatures	1833:1865	IgG1 specific N-glycan signatures that were predictive of PGD development	1833:1905	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	2	77	theme	recent	245:250	arg1	studies					252:258	recent studies	245:258	recent studies	245:258	The pathogenesis of COPD is complex; however, recent studies suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status.					
34767945	4	78	theme	altered	682:688	arg1	levels					715:720	altered circulating autoantibody levels	682:720	altered circulating autoantibody levels	682:720	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	9	79	theme	increased	1513:1521	arg1	recipients					1565:1574	compared PGD- recipients	1551:1574	compared PGD- recipients	1551:1574	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	9	79	theme	increased	1513:1521	arg1	signatures					1537:1546	significantly increased IgG1 N-glycan signatures	1499:1546	significantly increased IgG1 N-glycan signatures	1499:1546	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	6	80	theme	total	1004:1008	arg1	elastin					1011:1017	elastin	1011:1017	elastin	1011:1017	No significant differences were seen in total, elastin, or collagen IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies between PGD+ and PGD- recipients.					
34767945	4	81	theme	graft	653:657	arg1	PGD					672:674	PGD	672:674	PGD	672:674	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	4	81	theme	graft	653:657	arg1	dysfunction					659:669	primary graft dysfunction	645:669	primary graft dysfunction (PGD)	645:675	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	11	82	theme	IgG	1749:1751	arg1	analysis					1762:1769	a novel IgG glycomic analysis	1741:1769	a novel IgG glycomic analysis	1741:1769	However, using a novel IgG glycomic analysis we were able to demonstrate multiple significant increases in IgG1 specific N-glycan signatures that were predictive of PGD development.					
34767945	0	83	theme	COPD	92:95	arg1	patients					97:104	COPD patients	92:104	COPD patients	92:104	Pro-inflammatory IgG1 N-glycan signature correlates with primary graft dysfunction onset in COPD patients.					
34767945	9	84	theme	N-glycan	1528:1535	arg1	recipients					1565:1574	compared PGD- recipients	1551:1574	compared PGD- recipients	1551:1574	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	9	84	theme	N-glycan	1528:1535	arg1	signatures					1537:1546	significantly increased IgG1 N-glycan signatures	1499:1546	significantly increased IgG1 N-glycan signatures	1499:1546	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	12	85	theme	potential	1947:1955	arg1	method					1963:1968	a potential novel method	1945:1968	a potential novel method for identifying COPD patients at risk for PGD development and may provide clues to mechanisms by which antibody N-glycan signatures could contribute to antibody-mediated PGD pathogenesis	1945:2155	Taken together, these data represent a potential novel method for identifying COPD patients at risk for PGD development and may provide clues to mechanisms by which antibody N-glycan signatures could contribute to antibody-mediated PGD pathogenesis.					
34767945	9	86	theme	compared	1551:1558	arg1	recipients					1565:1574	compared PGD- recipients	1551:1574	compared PGD- recipients	1551:1574	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	9	86	theme	compared	1551:1558	arg1	signatures					1537:1546	significantly increased IgG1 N-glycan signatures	1499:1546	significantly increased IgG1 N-glycan signatures	1499:1546	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	9	87	theme	PGD-	1560:1563	arg1	recipients					1565:1574	compared PGD- recipients	1551:1574	compared PGD- recipients	1551:1574	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	9	87	theme	PGD-	1560:1563	arg1	signatures					1537:1546	significantly increased IgG1 N-glycan signatures	1499:1546	significantly increased IgG1 N-glycan signatures	1499:1546	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	7	88	theme	COPD	1277:1280	arg1	patients					1282:1289	COPD patients	1277:1289	COPD patients	1277:1289	Antibody function can be greatly altered by glycosylation changes to the antibody Fc region and recent studies have reported altered IgG glycosylation profiles in COPD patients.					
34767945	6	89	dep	elastin	1011:1017	arg1	antibodies					1069:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	No significant differences were seen in total, elastin, or collagen IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies between PGD+ and PGD- recipients.					
34767945	8	90	used	utilized	1305:1312	arg2	We					1292:1293	We	1292:1293	We	1292:1293	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	0	91	theme	IgG1	17:20	arg1	signature					31:39	Pro-inflammatory IgG1 N-glycan signature	0:39	Pro-inflammatory IgG1 N-glycan signature	0:39	Pro-inflammatory IgG1 N-glycan signature correlates with primary graft dysfunction onset in COPD patients.					
34767945	5	92	theme	lung	918:921	arg1	recipients					934:943	COPD lung transplant recipients	913:943	COPD lung transplant recipients pre- and post-LTx	913:961	We measured total immunoglobulin and circulating elastin and collagen autoantibody levels in a cohort of COPD lung transplant recipients pre- and post-LTx.					
34767945	6	93	theme	IgG	1037:1039	arg1	antibodies					1069:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	No significant differences were seen in total, elastin, or collagen IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies between PGD+ and PGD- recipients.					
34767945	4	94	theme	autoantibody	702:713	arg1	levels					715:720	altered circulating autoantibody levels	682:720	altered circulating autoantibody levels	682:720	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	1	95	theme	Chronic	107:113	arg1	COPD					146:149	COPD	146:149	COPD	146:149	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	1	95	theme	Chronic	107:113	arg1	disease					137:143	Chronic obstructive pulmonary disease	107:143	Chronic obstructive pulmonary disease (COPD)	107:150	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	1	95	theme	Chronic	107:113	arg1	cause					173:177	the third leading cause	155:177	the third leading cause of death worldwide	155:196	Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide.					
34767945	6	96	theme	IgM	1032:1034	arg1	antibodies					1069:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	No significant differences were seen in total, elastin, or collagen IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies between PGD+ and PGD- recipients.					
34767945	9	97	contain	had	1495:1497	arg2	signatures					1537:1546	significantly increased IgG1 N-glycan signatures	1499:1546	significantly increased IgG1 N-glycan signatures	1499:1546	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	9	97	contain	had	1495:1497	arg2	recipients					1565:1574	compared PGD- recipients	1551:1574	compared PGD- recipients	1551:1574	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	9	97	contain	had	1495:1497	arg1	recipients					1466:1475	COPD-LTx recipients	1457:1475	COPD-LTx recipients who developed PGD	1457:1493	COPD-LTx recipients who developed PGD had significantly increased IgG1 N-glycan signatures as compared PGD- recipients.					
34767945	8	98	theme	mass	1322:1325	arg1	approach					1381:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	10	99	theme	LTx	1693:1695	arg1	recipients					1697:1706	COPD LTx recipients	1688:1706	COPD LTx recipients that develop PGD	1688:1723	In conclusion, we show that immunoglobulin and autoreactive antibody levels are not significantly different in COPD LTx recipients that develop PGD.					
34767945	6	100	theme	IgG1	1042:1045	arg1	antibodies					1069:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies	1032:1078	No significant differences were seen in total, elastin, or collagen IgM, IgG, IgG1, IgG2, IgG3, and IgG4 antibodies between PGD+ and PGD- recipients.					
34767945	8	101	theme	multiplexed	1346:1356	arg1	approach					1381:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	0	102	theme	graft	65:69	arg1	onset					83:87	primary graft dysfunction onset	57:87	primary graft dysfunction onset in COPD patients	57:104	Pro-inflammatory IgG1 N-glycan signature correlates with primary graft dysfunction onset in COPD patients.					
34767945	8	103	theme	imaging	1373:1379	arg1	approach					1381:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	2	104	theme	autoantibodies	321:334	arg1	presence					309:316	the presence	305:316	the presence of autoantibodies to elastin and collagen	305:358	The pathogenesis of COPD is complex; however, recent studies suggest autoimmune changes, characterized by the presence of autoantibodies to elastin and collagen, may contribute to disease status.					
34767945	4	105	theme	COPD-LTx	763:770	arg1	recipients					772:781	those COPD-LTx recipients	757:781	those COPD-LTx recipients that do not develop PGD	757:805	We hypothesized that COPD patients that undergo LTx and develop primary graft dysfunction (PGD) have altered circulating autoantibody levels and phenotypic changes as compared those COPD-LTx recipients that do not develop PGD.					
34767945	8	106	gly	N-glycoprotein	1358:1371	arg1	N-glycoprotein					1358:1371	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach	1314:1388	We therefore utilized a novel mass spectrometry-based multiplexed N-glycoprotein imaging approach and measured changes in IgG-specific antibody N-glycan structures.					
34767945	7	107	theme	Fc	1196:1197	arg1	region					1199:1204	the antibody Fc region	1183:1204	the antibody Fc region	1183:1204	Antibody function can be greatly altered by glycosylation changes to the antibody Fc region and recent studies have reported altered IgG glycosylation profiles in COPD patients.					
34767945	7	108	theme	Antibody	1114:1121	arg1	function					1123:1130	Antibody function	1114:1130	Antibody function	1114:1130	Antibody function can be greatly altered by glycosylation changes to the antibody Fc region and recent studies have reported altered IgG glycosylation profiles in COPD patients.					
32546343	0	0	theme	autoimmune	91:100	arg1	cholangitis					102:112	autoimmune cholangitis	91:112	autoimmune cholangitis	91:112	Glycomic analysis of antibody indicates distinctive glycosylation profile in patients with autoimmune cholangitis.					
32546343	8	1	theme	potential	1434:1442	arg1	mechanism					1444:1452	a potential mechanism	1432:1452	a potential mechanism	1432:1452	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	8	1	theme	potential	1434:1442	arg1	importance					1400:1409	the importance	1396:1409	the importance of glycan biology	1396:1427	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	2	2	theme	residues	359:366	arg1	removal					322:328	The removal	318:328	The removal of sialic acid and galactose residues	318:366	The removal of sialic acid and galactose residues dramatically alters the physiological function of IgGs, and alterations of Ig glycosylation have been associated with several autoimmune disorders.					
32546343	1	3	theme	immune	250:255	arg1	quiescence					257:266	immune quiescence	250:266	immune quiescence	250:266	Glycosylation of antibodies, particularly in the Fc domain, critically modulate the ability of antibodies to bind to FcRs, maintaining immune quiescence to achieve a finely orchestrated immune response.					
32546343	2	4	theme	autoimmune	494:503	arg1	disorders					505:513	several autoimmune disorders	486:513	several autoimmune disorders	486:513	The removal of sialic acid and galactose residues dramatically alters the physiological function of IgGs, and alterations of Ig glycosylation have been associated with several autoimmune disorders.					
32546343	7	5	theme	PSC	1114:1116	arg1	patients					1118:1125	PSC patients	1114:1125	PSC patients	1114:1125	PSC patients had decreased bisecting glycoforms and increased biantennary glycoforms on IgA compared to PBC.					
32546343	2	6	theme	galactose	349:357	arg1	residues					359:366	galactose residues	349:366	galactose residues	349:366	The removal of sialic acid and galactose residues dramatically alters the physiological function of IgGs, and alterations of Ig glycosylation have been associated with several autoimmune disorders.					
32546343	6	7	theme	IgM	993:995	arg1	glycans					997:1003	IgM glycans	993:1003	IgM glycans at site N439	993:1016	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	8	8	theme	biology	1421:1427	arg1	signal					1480:1485	a disease specific signal	1461:1485	a disease specific signal of inflammation	1461:1501	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	8	8	theme	biology	1421:1427	arg1	mechanism					1444:1452	a potential mechanism	1432:1452	a potential mechanism	1432:1452	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	8	8	theme	biology	1421:1427	arg1	importance					1400:1409	the importance	1396:1409	the importance of glycan biology	1396:1427	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	6	9	from	glycans	1034:1040	arg1	chain					1047:1051	J chain	1045:1051	J chain	1045:1051	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	8	10	theme	glycan	1414:1419	arg1	biology					1421:1427	glycan biology	1414:1427	glycan biology	1414:1427	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	4	11	theme	biliary	806:812	arg1	PBC					827:829	PBC	827:829	PBC	827:829	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	11	theme	biliary	806:812	arg1	cholangitis					814:824	primary biliary cholangitis	798:824	primary biliary cholangitis (PBC)	798:830	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	3	12	theme	autoimmune	578:587	arg1	cholangitis					589:599	autoimmune cholangitis	578:599	autoimmune cholangitis	578:599	However, Ig glycosylation has not been extensively studied in autoimmune cholangitis.					
32546343	6	13	theme	glycans	997:1003	arg1	Levels					983:988	Levels	983:988	Levels	983:988	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	0	14	from	profile	66:72	arg1	patients					77:84	patients	77:84	patients with autoimmune cholangitis	77:112	Glycomic analysis of antibody indicates distinctive glycosylation profile in patients with autoimmune cholangitis.					
32546343	4	15	theme	primary	798:804	arg1	PBC					827:829	PBC	827:829	PBC	827:829	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	15	theme	primary	798:804	arg1	cholangitis					814:824	primary biliary cholangitis	798:824	primary biliary cholangitis (PBC)	798:830	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	0	16	with	patients	77:84	arg1	cholangitis					102:112	autoimmune cholangitis	91:112	autoimmune cholangitis	91:112	Glycomic analysis of antibody indicates distinctive glycosylation profile in patients with autoimmune cholangitis.					
32546343	3	17	gly	glycosylation	528:540	arg1	cholangitis					589:599	autoimmune cholangitis	578:599	autoimmune cholangitis	578:599	However, Ig glycosylation has not been extensively studied in autoimmune cholangitis.					
32546343	4	18	theme	triple	613:618	arg1	spectroscopy					635:646	triple quadruple mass spectroscopy	613:646	triple quadruple mass spectroscopy with subsequent multiple reaction monitoring	613:691	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	19	theme	residues	752:759	arg1	linkage					735:741	linkage	735:741	linkage	735:741	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	19	theme	residues	752:759	arg1	composition					719:729	composition	719:729	composition	719:729	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	19	theme	residues	752:759	arg1	profile					710:716	the profile	706:716	the profile	706:716	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	6	20	from	glycans	997:1003	arg1	chain					1047:1051	J chain	1045:1051	J chain	1045:1051	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	4	21	theme	multiple	664:671	arg1	monitoring					682:691	subsequent multiple reaction monitoring	653:691	subsequent multiple reaction monitoring	653:691	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	8	22	theme	disease	1342:1348	arg1	severity					1350:1357	disease severity	1342:1357	disease severity	1342:1357	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	1	23	gly	Glycosylation	115:127	arg1	antibodies					132:141	antibodies	132:141	antibodies	132:141	Glycosylation of antibodies, particularly in the Fc domain, critically modulate the ability of antibodies to bind to FcRs, maintaining immune quiescence to achieve a finely orchestrated immune response.					
32546343	8	24	gly	glycoforms	1326:1335	arg1	Ig					1323:1324	Ig glycoforms	1323:1335	Ig glycoforms	1323:1335	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	2	25	theme	several	486:492	arg1	disorders					505:513	several autoimmune disorders	486:513	several autoimmune disorders	486:513	The removal of sialic acid and galactose residues dramatically alters the physiological function of IgGs, and alterations of Ig glycosylation have been associated with several autoimmune disorders.					
32546343	0	26	theme	Glycomic	0:7	arg1	analysis					9:16	Glycomic analysis	0:16	Glycomic analysis of antibody	0:28	Glycomic analysis of antibody indicates distinctive glycosylation profile in patients with autoimmune cholangitis.					
32546343	6	27	from	site	1008:1011	arg1	Levels					983:988	Levels	983:988	Levels	983:988	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	6	27	from	site	1008:1011	arg1	glycans					997:1003	IgM glycans	993:1003	IgM glycans at site N439	993:1016	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	6	27	from	site	1008:1011	arg1	glycans					1034:1040	fucosylated glycans	1022:1040	fucosylated glycans in J chain	1022:1051	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	4	28	from	patients	784:791	arg1	residues					752:759	sugar residues	746:759	sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC)	746:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	28	from	patients	784:791	arg1	glycans					773:779	antibody glycans	764:779	antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC)	764:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	2	29	theme	physiological	392:404	arg1	function					406:413	the physiological function	388:413	the physiological function of IgGs	388:421	The removal of sialic acid and galactose residues dramatically alters the physiological function of IgGs, and alterations of Ig glycosylation have been associated with several autoimmune disorders.					
32546343	2	30	theme	glycosylation	446:458	arg1	alterations					428:438	alterations	428:438	alterations of Ig glycosylation	428:458	The removal of sialic acid and galactose residues dramatically alters the physiological function of IgGs, and alterations of Ig glycosylation have been associated with several autoimmune disorders.					
32546343	4	31	theme	healthy	874:880	arg1	HC					892:893	HC	892:893	HC	892:893	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	31	theme	healthy	874:880	arg1	controls					882:889	healthy controls	874:889	healthy controls (HC)	874:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	32	theme	glycans	773:779	arg1	residues					752:759	sugar residues	746:759	sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC)	746:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	32	theme	glycans	773:779	arg1	glycans					773:779	antibody glycans	764:779	antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC)	764:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	8	33	theme	inflammation	1490:1501	arg1	signal					1480:1485	a disease specific signal	1461:1485	a disease specific signal of inflammation	1461:1501	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	8	33	theme	inflammation	1490:1501	arg1	importance					1400:1409	the importance	1396:1409	the importance of glycan biology	1396:1427	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	2	34	theme	Ig	443:444	arg1	glycosylation					446:458	Ig glycosylation	443:458	Ig glycosylation	443:458	The removal of sialic acid and galactose residues dramatically alters the physiological function of IgGs, and alterations of Ig glycosylation have been associated with several autoimmune disorders.					
32546343	8	35	theme	patterns	1311:1318	arg1	association					1261:1271	the association	1257:1271	the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation	1257:1501	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	5	36	gly	glycoforms	937:946	arg1	IgG1					932:935	Agalactosylated, HexNAc terminated IgG1 glycoforms	897:946	Agalactosylated, HexNAc terminated IgG1 glycoforms	897:946	Agalactosylated, HexNAc terminated IgG1 glycoforms were enriched in both PBC and PSC.					
32546343	4	37	from	glycans	773:779	arg1	patients					784:791	patients	784:791	patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC)	784:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	5	38	dep	Agalactosylated	897:911	arg1	terminated					921:930	terminated	921:930	terminated	921:930	Agalactosylated, HexNAc terminated IgG1 glycoforms were enriched in both PBC and PSC.					
32546343	4	39	theme	reaction	673:680	arg1	monitoring					682:691	subsequent multiple reaction monitoring	653:691	subsequent multiple reaction monitoring	653:691	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	1	40	theme	orchestrated	288:299	arg1	response					308:315	a finely orchestrated immune response	279:315	a finely orchestrated immune response	279:315	Glycosylation of antibodies, particularly in the Fc domain, critically modulate the ability of antibodies to bind to FcRs, maintaining immune quiescence to achieve a finely orchestrated immune response.					
32546343	8	41	theme	liver	1363:1367	arg1	cirrhosis					1369:1377	liver cirrhosis	1363:1377	liver cirrhosis	1363:1377	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	7	42	from	glycoforms	1188:1197	arg1	IgA					1202:1204	IgA	1202:1204	IgA	1202:1204	PSC patients had decreased bisecting glycoforms and increased biantennary glycoforms on IgA compared to PBC.					
32546343	3	43	theme	Ig	525:526	arg1	glycosylation					528:540	Ig glycosylation	525:540	Ig glycosylation	525:540	However, Ig glycosylation has not been extensively studied in autoimmune cholangitis.					
32546343	1	44	theme	antibodies	210:219	arg1	ability					199:205	the ability	195:205	the ability of antibodies to bind to FcRs	195:235	Glycosylation of antibodies, particularly in the Fc domain, critically modulate the ability of antibodies to bind to FcRs, maintaining immune quiescence to achieve a finely orchestrated immune response.					
32546343	0	45	theme	distinctive	40:50	arg1	profile					66:72	distinctive glycosylation profile	40:72	distinctive glycosylation profile in patients with autoimmune cholangitis	40:112	Glycomic analysis of antibody indicates distinctive glycosylation profile in patients with autoimmune cholangitis.					
32546343	1	46	theme	immune	301:306	arg1	response					308:315	a finely orchestrated immune response	279:315	a finely orchestrated immune response	279:315	Glycosylation of antibodies, particularly in the Fc domain, critically modulate the ability of antibodies to bind to FcRs, maintaining immune quiescence to achieve a finely orchestrated immune response.					
32546343	4	47	dep	triple	613:618	arg1	quadruple					620:628	quadruple	620:628	quadruple	620:628	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	6	48	theme	J	1045:1045	arg1	chain					1047:1051	J chain	1045:1051	J chain	1045:1051	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	7	49	theme	bisecting	1141:1149	arg1	glycoforms					1151:1160	bisecting glycoforms	1141:1160	bisecting glycoforms	1141:1160	PSC patients had decreased bisecting glycoforms and increased biantennary glycoforms on IgA compared to PBC.					
32546343	6	50	from	Levels	983:988	arg1	chain					1047:1051	J chain	1045:1051	J chain	1045:1051	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	6	50	from	Levels	983:988	arg1	site					1008:1011	site N439	1008:1016	site N439	1008:1016	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	8	51	theme	disease	1463:1469	arg1	signal					1480:1485	a disease specific signal	1461:1485	a disease specific signal of inflammation	1461:1501	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	8	51	theme	disease	1463:1469	arg1	importance					1400:1409	the importance	1396:1409	the importance of glycan biology	1396:1427	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	0	52	theme	antibody	21:28	arg1	analysis					9:16	Glycomic analysis	0:16	Glycomic analysis of antibody	0:28	Glycomic analysis of antibody indicates distinctive glycosylation profile in patients with autoimmune cholangitis.					
32546343	2	53	theme	IgGs	418:421	arg1	function					406:413	the physiological function	388:413	the physiological function of IgGs	388:421	The removal of sialic acid and galactose residues dramatically alters the physiological function of IgGs, and alterations of Ig glycosylation have been associated with several autoimmune disorders.					
32546343	4	54	with	patients	784:791	arg1	controls					882:889	healthy controls	874:889	healthy controls (HC)	874:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	54	with	patients	784:791	arg1	cholangitis					814:824	primary biliary cholangitis	798:824	primary biliary cholangitis (PBC)	798:830	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	54	with	patients	784:791	arg1	cholangitis					852:862	primary sclerosing cholangitis	833:862	primary sclerosing cholangitis (PSC)	833:868	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	54	with	patients	784:791	arg1	PSC					865:867	PSC	865:867	PSC	865:867	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	54	with	patients	784:791	arg1	HC					892:893	HC	892:893	HC	892:893	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	54	with	patients	784:791	arg1	PBC					827:829	PBC	827:829	PBC	827:829	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	6	55	from	chain	1047:1051	arg1	Levels					983:988	Levels	983:988	Levels	983:988	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	4	56	with	spectroscopy	635:646	arg1	monitoring					682:691	subsequent multiple reaction monitoring	653:691	subsequent multiple reaction monitoring	653:691	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	8	57	with	association	1261:1271	arg1	severity					1350:1357	disease severity	1342:1357	disease severity	1342:1357	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	8	57	with	association	1261:1271	arg1	cirrhosis					1369:1377	liver cirrhosis	1363:1377	liver cirrhosis	1363:1377	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	1	58	theme	antibodies	132:141	arg1	Glycosylation					115:127	Glycosylation	115:127	Glycosylation	115:127	Glycosylation of antibodies, particularly in the Fc domain, critically modulate the ability of antibodies to bind to FcRs, maintaining immune quiescence to achieve a finely orchestrated immune response.					
32546343	0	59	theme	glycosylation	52:64	arg1	profile					66:72	distinctive glycosylation profile	40:72	distinctive glycosylation profile in patients with autoimmune cholangitis	40:112	Glycomic analysis of antibody indicates distinctive glycosylation profile in patients with autoimmune cholangitis.					
32546343	8	60	theme	glycoforms	1326:1335	arg1	branching					1285:1293	distinct branching and composition patterns	1276:1318	branching	1285:1293	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	8	60	theme	glycoforms	1326:1335	arg1	patterns					1311:1318	distinct branching and composition patterns	1276:1318	patterns	1311:1318	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	2	61	theme	acid	340:343	arg1	removal					322:328	The removal	318:328	The removal of sialic acid and galactose residues	318:366	The removal of sialic acid and galactose residues dramatically alters the physiological function of IgGs, and alterations of Ig glycosylation have been associated with several autoimmune disorders.					
32546343	1	62	from	Glycosylation	115:127	arg1	domain					167:172	the Fc domain	160:172	the Fc domain	160:172	Glycosylation of antibodies, particularly in the Fc domain, critically modulate the ability of antibodies to bind to FcRs, maintaining immune quiescence to achieve a finely orchestrated immune response.					
32546343	6	63	theme	fucosylated	1022:1032	arg1	glycans					1034:1040	fucosylated glycans	1022:1040	fucosylated glycans in J chain	1022:1051	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	8	64	theme	distinct	1276:1283	arg1	branching					1285:1293	distinct branching and composition patterns	1276:1318	branching	1285:1293	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	8	65	theme	specific	1471:1478	arg1	signal					1480:1485	a disease specific signal	1461:1485	a disease specific signal of inflammation	1461:1501	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	8	65	theme	specific	1471:1478	arg1	importance					1400:1409	the importance	1396:1409	the importance of glycan biology	1396:1427	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	4	66	theme	sclerosing	841:850	arg1	cholangitis					852:862	primary sclerosing cholangitis	833:862	primary sclerosing cholangitis (PSC)	833:868	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	66	theme	sclerosing	841:850	arg1	PSC					865:867	PSC	865:867	PSC	865:867	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	2	67	theme	sialic	333:338	arg1	acid					340:343	sialic acid	333:343	sialic acid	333:343	The removal of sialic acid and galactose residues dramatically alters the physiological function of IgGs, and alterations of Ig glycosylation have been associated with several autoimmune disorders.					
32546343	6	68	theme	glycans	1034:1040	arg1	Levels					983:988	Levels	983:988	Levels	983:988	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	7	69	gly	glycoforms	1188:1197	arg2	IgA					1202:1204	IgA	1202:1204	IgA	1202:1204	PSC patients had decreased bisecting glycoforms and increased biantennary glycoforms on IgA compared to PBC.					
32546343	5	70	theme	IgG1	932:935	arg1	glycoforms					937:946	Agalactosylated, HexNAc terminated IgG1 glycoforms	897:946	Agalactosylated, HexNAc terminated IgG1 glycoforms	897:946	Agalactosylated, HexNAc terminated IgG1 glycoforms were enriched in both PBC and PSC.					
32546343	8	71	theme	Ig	1323:1324	arg1	glycoforms					1326:1335	Ig glycoforms	1323:1335	Ig glycoforms	1323:1335	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	6	72	gly	fucosylated	1022:1032	arg1	glycans					1034:1040	fucosylated glycans	1022:1040	fucosylated glycans in J chain	1022:1051	Levels of IgM glycans at site N439 and fucosylated glycans in J chain, were significantly decreased in PBC compared to PSC and HC.					
32546343	8	73	theme	composition	1299:1309	arg1	patterns					1311:1318	distinct branching and composition patterns	1276:1318	patterns	1311:1318	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	4	74	from	residues	752:759	arg1	patients					784:791	patients	784:791	patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC)	784:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	75	theme	antibody	764:771	arg1	glycans					773:779	antibody glycans	764:779	antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC)	764:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	8	76	theme	branching	1285:1293	arg1	association					1261:1271	the association	1257:1271	the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation	1257:1501	Importantly, our data demonstrate the association of distinct branching and composition patterns of Ig glycoforms with disease severity and liver cirrhosis, which highlight the importance of glycan biology as a potential mechanism and/or a disease specific signal of inflammation.					
32546343	4	77	theme	primary	833:839	arg1	cholangitis					852:862	primary sclerosing cholangitis	833:862	primary sclerosing cholangitis (PSC)	833:868	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	77	theme	primary	833:839	arg1	PSC					865:867	PSC	865:867	PSC	865:867	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	5	78	theme	Agalactosylated	897:911	arg1	glycoforms					937:946	Agalactosylated, HexNAc terminated IgG1 glycoforms	897:946	Agalactosylated, HexNAc terminated IgG1 glycoforms	897:946	Agalactosylated, HexNAc terminated IgG1 glycoforms were enriched in both PBC and PSC.					
32546343	4	79	theme	subsequent	653:662	arg1	monitoring					682:691	subsequent multiple reaction monitoring	653:691	subsequent multiple reaction monitoring	653:691	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	7	80	gly	glycoforms	1151:1160	arg1	bisecting					1141:1149	bisecting glycoforms	1141:1160	bisecting glycoforms	1141:1160	PSC patients had decreased bisecting glycoforms and increased biantennary glycoforms on IgA compared to PBC.					
32546343	4	81	theme	mass	630:633	arg1	spectroscopy					635:646	triple quadruple mass spectroscopy	613:646	triple quadruple mass spectroscopy with subsequent multiple reaction monitoring	613:691	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	1	82	theme	Fc	164:165	arg1	domain					167:172	the Fc domain	160:172	the Fc domain	160:172	Glycosylation of antibodies, particularly in the Fc domain, critically modulate the ability of antibodies to bind to FcRs, maintaining immune quiescence to achieve a finely orchestrated immune response.					
32546343	7	83	theme	biantennary	1176:1186	arg1	glycoforms					1188:1197	biantennary glycoforms	1176:1197	biantennary glycoforms on IgA	1176:1204	PSC patients had decreased bisecting glycoforms and increased biantennary glycoforms on IgA compared to PBC.					
32546343	4	84	theme	sugar	746:750	arg1	residues					752:759	sugar residues	746:759	sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC)	746:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
32546343	4	84	theme	sugar	746:750	arg1	glycans					773:779	antibody glycans	764:779	antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC)	764:894	We applied triple quadruple mass spectroscopy with subsequent multiple reaction monitoring to elucidate the profile, composition and linkage of sugar residues of antibody glycans in patients with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC) and healthy controls (HC).					
33586190	2	0	theme	bonding	615:621	arg1	interactions					623:634	van der Waals forces and hydrogen bonding interactions	581:634	interactions	623:634	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	10	1	theme	imminent	2038:2045	arg1	features					2010:2017	the distinguished features	1992:2017	the distinguished features of V-amylose	1992:2030	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	10	1	theme	imminent	2038:2045	arg1	system					2061:2066	an imminent drug delivery system	2035:2066	an imminent drug delivery system	2035:2066	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	2	2	theme	glycosidic	364:373	arg1	linkages					375:382	the glycosidic linkages	360:382	the glycosidic linkages	360:382	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	1	3	theme	enzymatic	268:276	arg1	hydrolysis					278:287	enzymatic hydrolysis	268:287	enzymatic hydrolysis	268:287	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	6	4	theme	wide	1249:1252	arg1	range					1254:1258	a wide range	1247:1258	a wide range of therapeutics	1247:1274	These structural features of V-amylose differentiate it from the parent amylose polysaccharide and enable the accommodation and nanoencapsulation of a wide range of therapeutics in the former.					
33586190	3	5	theme	drug	743:746	arg1	molecules					748:756	the cargo drug molecules	733:756	the cargo drug molecules	733:756	These properties enable a high-loading efficiency, targeted delivery, and controlled release of the cargo drug molecules by V-amylose.					
33586190	9	6	theme	single	1780:1785	arg1	V-amylose					1795:1803	The single helical V-amylose	1776:1803	The single helical V-amylose with hydrophobic core and hydrophilic periphery	1776:1851	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	7	7	theme	release	1508:1514	arg1	systems					1516:1522	controlled drug release systems	1492:1522	controlled drug release systems	1492:1522	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	7	7	theme	release	1508:1514	arg1	application					1464:1474	the application	1460:1474	the application of V-amylose	1460:1487	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	4	8	theme	physiological	876:888	arg1	benevolence					890:900	physiological benevolence	876:900	physiological benevolence	876:900	Besides, V-amylose presents characteristics of an ideal drug delivery system, such as biocompatibility, physiological benevolence, nonimmunogenicity, and biodegradability.					
33586190	7	9	theme	controlled	1492:1501	arg1	systems					1516:1522	controlled drug release systems	1492:1522	controlled drug release systems	1492:1522	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	7	9	theme	controlled	1492:1501	arg1	application					1464:1474	the application	1460:1474	the application of V-amylose	1460:1487	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	2	10	theme	molecules	567:575	arg1	range					558:562	a diverse range	548:562	a diverse range of molecules	548:575	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	7	11	theme	V-amylose	1479:1487	arg1	systems					1516:1522	controlled drug release systems	1492:1522	controlled drug release systems	1492:1522	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	7	11	theme	V-amylose	1479:1487	arg1	application					1464:1474	the application	1460:1474	the application of V-amylose	1460:1487	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	5	12	theme	left-handed	990:1000	arg1	helix					1009:1013	left-handed single helix	990:1013	left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å	990:1095	The V-amylose polysaccharide chains fold into left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å.					
33586190	9	13	dep	absorption	1896:1905	arg1	the					1892:1894	the	1892:1894	the	1892:1894	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	2	14	theme	range	558:562	arg1	loading					537:543	the loading	533:543	the loading of a diverse range of molecules	533:575	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	2	15	theme	Waals	589:593	arg1	forces					595:600	van der Waals forces and hydrogen bonding interactions	581:634	forces	595:600	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	2	16	theme	van	581:583	arg1	forces					595:600	van der Waals forces and hydrogen bonding interactions	581:634	forces	595:600	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	9	17	contain	having	1931:1936	arg2	index					1951:1955	a high clogP index	1938:1955	a high clogP index	1938:1955	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	9	17	contain	having	1931:1936	arg1	drugs					1925:1929	drugs	1925:1929	drugs having a high clogP index	1925:1955	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	5	18	theme	polysaccharide	958:971	arg1	chains					973:978	The V-amylose polysaccharide chains	944:978	The V-amylose polysaccharide chains	944:978	The V-amylose polysaccharide chains fold into left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å.					
33586190	9	19	theme	inclusion	1859:1867	arg1	complexes					1869:1877	inclusion complexes	1859:1877	inclusion complexes that improve the absorption and permeation of drugs having a high clogP index	1859:1955	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	8	20	theme	enzyme	1553:1558	arg1	activity					1529:1536	The activity	1525:1536	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles	1525:1680	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	7	21	theme	linear	1421:1426	arg1	polysaccharides					1428:1442	the linear polysaccharides	1417:1442	the linear polysaccharides	1417:1442	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	2	22	theme	helix	446:450	arg1	cavity					426:431	the hydrophobic cavity	410:431	the hydrophobic cavity of V-amylose helix	410:450	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	2	23	theme	hydrophilic	503:513	arg1	periphery					515:523	its hydrophilic periphery	499:523	its hydrophilic periphery	499:523	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	10	24	theme	distinguished	1996:2008	arg1	system					2061:2066	an imminent drug delivery system	2035:2066	an imminent drug delivery system	2035:2066	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	10	24	theme	distinguished	1996:2008	arg1	features					2010:2017	the distinguished features	1992:2017	the distinguished features of V-amylose	1992:2030	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	1	25	theme	V-amylose	62:70	arg1	structure					49:57	The helical structure	37:57	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis	37:287	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	8	26	theme	gastrointestinal	1599:1614	arg1	tract					1616:1620	gastrointestinal tract	1599:1620	gastrointestinal tract	1599:1620	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	5	27	theme	glucose	1033:1039	arg1	units					1041:1045	six glucose units	1029:1045	six glucose units	1029:1045	The V-amylose polysaccharide chains fold into left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å.					
33586190	0	28	theme	Therapeutic	0:10	arg1	delivery					12:19	Therapeutic delivery	0:19	Therapeutic delivery with V-amylose.	0:35	Therapeutic delivery with V-amylose.					
33586190	1	29	theme	encapsulation	92:104	arg1	affinity					106:113	a superior encapsulation affinity	81:113	a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules;	81:214	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	6	30	theme	structural	1104:1113	arg1	features					1115:1122	These structural features	1098:1122	These structural features of V-amylose	1098:1135	These structural features of V-amylose differentiate it from the parent amylose polysaccharide and enable the accommodation and nanoencapsulation of a wide range of therapeutics in the former.					
33586190	9	31	theme	high	1940:1943	arg1	index					1951:1955	a high clogP index	1938:1955	a high clogP index	1938:1955	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	8	32	theme	gut	1627:1629	arg1	microbiota					1631:1640	gut microbiota	1627:1640	gut microbiota	1627:1640	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	2	33	theme	V-amylose	436:444	arg1	helix					446:450	V-amylose helix	436:450	V-amylose helix	436:450	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	4	34	theme	system	842:847	arg1	biocompatibility					858:873	biocompatibility	858:873	biocompatibility	858:873	Besides, V-amylose presents characteristics of an ideal drug delivery system, such as biocompatibility, physiological benevolence, nonimmunogenicity, and biodegradability.					
33586190	4	34	theme	system	842:847	arg1	nonimmunogenicity					903:919	nonimmunogenicity	903:919	nonimmunogenicity	903:919	Besides, V-amylose presents characteristics of an ideal drug delivery system, such as biocompatibility, physiological benevolence, nonimmunogenicity, and biodegradability.					
33586190	4	34	theme	system	842:847	arg1	biodegradability					926:941	biodegradability	926:941	biodegradability	926:941	Besides, V-amylose presents characteristics of an ideal drug delivery system, such as biocompatibility, physiological benevolence, nonimmunogenicity, and biodegradability.					
33586190	4	34	theme	system	842:847	arg1	benevolence					890:900	physiological benevolence	876:900	physiological benevolence	876:900	Besides, V-amylose presents characteristics of an ideal drug delivery system, such as biocompatibility, physiological benevolence, nonimmunogenicity, and biodegradability.					
33586190	4	34	theme	system	842:847	arg1	characteristics					800:814	characteristics	800:814	characteristics	800:814	Besides, V-amylose presents characteristics of an ideal drug delivery system, such as biocompatibility, physiological benevolence, nonimmunogenicity, and biodegradability.					
33586190	1	35	theme	potential	319:327	arg1	applications					301:312	its applications	297:312	its applications	297:312	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	1	35	theme	potential	319:327	arg1	vehicle					343:349	a potential drug delivery vehicle	317:349	a potential drug delivery vehicle	317:349	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	2	36	theme	hydroxyl	470:477	arg1	groups					479:484	the glycosyl hydroxyl groups	457:484	the glycosyl hydroxyl groups constituting its hydrophilic periphery	457:523	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	8	37	theme	drug	1659:1662	arg1	vehicles					1673:1680	amylose-based drug delivery vehicles	1645:1680	amylose-based drug delivery vehicles	1645:1680	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	9	38	with	V-amylose	1795:1803	arg1	core					1822:1825	hydrophobic core	1810:1825	hydrophobic core	1810:1825	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	9	38	with	V-amylose	1795:1803	arg1	periphery					1843:1851	hydrophilic periphery	1831:1851	hydrophilic periphery	1831:1851	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	1	39	theme	delivery	334:341	arg1	applications					301:312	its applications	297:312	its applications	297:312	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	1	39	theme	delivery	334:341	arg1	vehicle					343:349	a potential drug delivery vehicle	317:349	a potential drug delivery vehicle	317:349	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	7	40	theme	extraordinary	1350:1362	arg1	resistance					1364:1373	extraordinary resistance	1350:1373	extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems	1350:1522	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	10	41	theme	V-amylose	2022:2030	arg1	system					2061:2066	an imminent drug delivery system	2035:2066	an imminent drug delivery system	2035:2066	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	10	41	theme	V-amylose	2022:2030	arg1	features					2010:2017	the distinguished features	1992:2017	the distinguished features of V-amylose	1992:2030	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	8	42	theme	encapsulated	1757:1768	arg1	drug					1770:1773	the encapsulated drug	1753:1773	the encapsulated drug	1753:1773	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	7	43	theme	V-amylose	1331:1339	arg1	structure					1318:1326	The tightly packed helical structure	1291:1326	The tightly packed helical structure of V-amylose	1291:1339	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	3	44	theme	targeted	688:695	arg1	delivery					697:704	targeted delivery	688:704	targeted delivery	688:704	These properties enable a high-loading efficiency, targeted delivery, and controlled release of the cargo drug molecules by V-amylose.					
33586190	4	45	theme	drug	828:831	arg1	system					842:847	an ideal drug delivery system	819:847	an ideal drug delivery system	819:847	Besides, V-amylose presents characteristics of an ideal drug delivery system, such as biocompatibility, physiological benevolence, nonimmunogenicity, and biodegradability.					
33586190	1	46	theme	higher	243:248	arg1	resistance					250:259	a higher resistance	241:259	a higher resistance toward enzymatic hydrolysis	241:287	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	8	47	theme	oral	1718:1721	arg1	release					1742:1748	enzyme-sensitive controlled oral and colon-specific release	1690:1748	enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug	1690:1773	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	10	48	theme	drug	2047:2050	arg1	features					2010:2017	the distinguished features	1992:2017	the distinguished features of V-amylose	1992:2030	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	10	48	theme	drug	2047:2050	arg1	system					2061:2066	an imminent drug delivery system	2035:2066	an imminent drug delivery system	2035:2066	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	2	49	theme	hydrogen	606:613	arg1	interactions					623:634	van der Waals forces and hydrogen bonding interactions	581:634	interactions	623:634	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	7	50	theme	packed	1303:1308	arg1	structure					1318:1326	The tightly packed helical structure	1291:1326	The tightly packed helical structure of V-amylose	1291:1339	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	8	51	theme	colon-specific	1727:1740	arg1	release					1742:1748	enzyme-sensitive controlled oral and colon-specific release	1690:1748	enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug	1690:1773	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	3	52	theme	controlled	711:720	arg1	release					722:728	controlled release	711:728	controlled release of the cargo drug molecules by V-amylose	711:769	These properties enable a high-loading efficiency, targeted delivery, and controlled release of the cargo drug molecules by V-amylose.					
33586190	3	53	theme	molecules	748:756	arg1	release					722:728	controlled release	711:728	controlled release of the cargo drug molecules by V-amylose	711:769	These properties enable a high-loading efficiency, targeted delivery, and controlled release of the cargo drug molecules by V-amylose.					
33586190	3	53	theme	molecules	748:756	arg1	efficiency					676:685	a high-loading efficiency	661:685	a high-loading efficiency	661:685	These properties enable a high-loading efficiency, targeted delivery, and controlled release of the cargo drug molecules by V-amylose.					
33586190	3	53	theme	molecules	748:756	arg1	delivery					697:704	targeted delivery	688:704	targeted delivery	688:704	These properties enable a high-loading efficiency, targeted delivery, and controlled release of the cargo drug molecules by V-amylose.					
33586190	1	54	theme	amphiphilic	178:188	arg1	molecules					205:213	the amphiphilic or hydrophobic molecules	174:213	molecules	205:213	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	2	55	theme	glycosyl	461:468	arg1	groups					479:484	the glycosyl hydroxyl groups	457:484	the glycosyl hydroxyl groups constituting its hydrophilic periphery	457:523	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	9	56	theme	helical	1787:1793	arg1	V-amylose					1795:1803	The single helical V-amylose	1776:1803	The single helical V-amylose with hydrophobic core and hydrophilic periphery	1776:1851	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	3	57	theme	cargo	737:741	arg1	molecules					748:756	the cargo drug molecules	733:756	the cargo drug molecules	733:756	These properties enable a high-loading efficiency, targeted delivery, and controlled release of the cargo drug molecules by V-amylose.					
33586190	1	58	theme	hydrophobic	193:203	arg1	molecules					205:213	the amphiphilic or hydrophobic molecules	174:213	molecules	205:213	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	7	59	theme	drug	1503:1506	arg1	systems					1516:1522	controlled drug release systems	1492:1522	controlled drug release systems	1492:1522	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	7	59	theme	drug	1503:1506	arg1	application					1464:1474	the application	1460:1474	the application of V-amylose	1460:1487	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	6	60	dep	accommodation	1208:1220	arg1	the					1204:1206	the	1204:1206	the	1204:1206	These structural features of V-amylose differentiate it from the parent amylose polysaccharide and enable the accommodation and nanoencapsulation of a wide range of therapeutics in the former.					
33586190	9	61	theme	hydrophilic	1831:1841	arg1	periphery					1843:1851	hydrophilic periphery	1831:1851	hydrophilic periphery	1831:1851	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	1	62	dep	providing	231:239	arg1	addition					219:226	addition	219:226	addition	219:226	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	5	63	theme	V-amylose	948:956	arg1	chains					973:978	The V-amylose polysaccharide chains	944:978	The V-amylose polysaccharide chains	944:978	The V-amylose polysaccharide chains fold into left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å.					
33586190	2	64	theme	der	585:587	arg1	forces					595:600	van der Waals forces and hydrogen bonding interactions	581:634	forces	595:600	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	5	65	theme	7.91-8.17 Å	1085:1095	arg1	height					1075:1080	a pitch height	1067:1080	a pitch height of 7.91-8.17 Å	1067:1095	The V-amylose polysaccharide chains fold into left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å.					
33586190	10	66	theme	present	1962:1968	arg1	commentary					1970:1979	The present commentary	1958:1979	The present commentary	1958:1979	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	1	67	theme	other	133:137	arg1	polysaccharides					139:153	the other polysaccharides	129:153	the other polysaccharides	129:153	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	8	68	theme	salivary	1572:1579	arg1	glands					1581:1586	salivary glands	1572:1586	salivary glands	1572:1586	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	6	69	theme	therapeutics	1263:1274	arg1	range					1254:1258	a wide range	1247:1258	a wide range of therapeutics	1247:1274	These structural features of V-amylose differentiate it from the parent amylose polysaccharide and enable the accommodation and nanoencapsulation of a wide range of therapeutics in the former.					
33586190	6	70	theme	range	1254:1258	arg1	accommodation					1208:1220	accommodation	1208:1220	accommodation	1208:1220	These structural features of V-amylose differentiate it from the parent amylose polysaccharide and enable the accommodation and nanoencapsulation of a wide range of therapeutics in the former.					
33586190	6	70	theme	range	1254:1258	arg1	nanoencapsulation					1226:1242	nanoencapsulation	1226:1242	nanoencapsulation	1226:1242	These structural features of V-amylose differentiate it from the parent amylose polysaccharide and enable the accommodation and nanoencapsulation of a wide range of therapeutics in the former.					
33586190	6	71	theme	parent	1163:1168	arg1	polysaccharide					1178:1191	the parent amylose polysaccharide	1159:1191	the parent amylose polysaccharide	1159:1191	These structural features of V-amylose differentiate it from the parent amylose polysaccharide and enable the accommodation and nanoencapsulation of a wide range of therapeutics in the former.					
33586190	6	72	theme	V-amylose	1127:1135	arg1	features					1115:1122	These structural features	1098:1122	These structural features of V-amylose	1098:1135	These structural features of V-amylose differentiate it from the parent amylose polysaccharide and enable the accommodation and nanoencapsulation of a wide range of therapeutics in the former.					
33586190	9	73	theme	drugs	1925:1929	arg1	permeation					1911:1920	permeation	1911:1920	permeation	1911:1920	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	9	73	theme	drugs	1925:1929	arg1	absorption					1896:1905	absorption	1896:1905	absorption	1896:1905	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	1	74	theme	superior	83:90	arg1	affinity					106:113	a superior encapsulation affinity	81:113	a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules;	81:214	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	5	75	theme	single	1002:1007	arg1	helix					1009:1013	left-handed single helix	990:1013	left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å	990:1095	The V-amylose polysaccharide chains fold into left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å.					
33586190	9	76	theme	clogP	1945:1949	arg1	index					1951:1955	a high clogP index	1938:1955	a high clogP index	1938:1955	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	8	77	theme	amylose-based	1645:1657	arg1	vehicles					1673:1680	amylose-based drug delivery vehicles	1645:1680	amylose-based drug delivery vehicles	1645:1680	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	3	78	theme	high-loading	663:674	arg1	efficiency					676:685	a high-loading efficiency	661:685	a high-loading efficiency	661:685	These properties enable a high-loading efficiency, targeted delivery, and controlled release of the cargo drug molecules by V-amylose.					
33586190	8	79	theme	delivery	1664:1671	arg1	vehicles					1673:1680	amylose-based drug delivery vehicles	1645:1680	amylose-based drug delivery vehicles	1645:1680	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	4	80	theme	delivery	833:840	arg1	system					842:847	an ideal drug delivery system	819:847	an ideal drug delivery system	819:847	Besides, V-amylose presents characteristics of an ideal drug delivery system, such as biocompatibility, physiological benevolence, nonimmunogenicity, and biodegradability.					
33586190	2	81	theme	hydrophobic	414:424	arg1	cavity					426:431	the hydrophobic cavity	410:431	the hydrophobic cavity of V-amylose helix	410:450	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	1	82	theme	drug	329:332	arg1	applications					301:312	its applications	297:312	its applications	297:312	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	1	82	theme	drug	329:332	arg1	vehicle					343:349	a potential drug delivery vehicle	317:349	a potential drug delivery vehicle	317:349	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	5	83	theme	pitch	1069:1073	arg1	height					1075:1080	a pitch height	1067:1080	a pitch height of 7.91-8.17 Å	1067:1095	The V-amylose polysaccharide chains fold into left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å.					
33586190	2	84	theme	diverse	550:556	arg1	range					558:562	a diverse range	548:562	a diverse range of molecules	548:575	Mainly, the glycosidic linkages and -CH2 - groups forming the hydrophobic cavity of V-amylose helix, and the glycosyl hydroxyl groups constituting its hydrophilic periphery promote the loading of a diverse range of molecules via van der Waals forces and hydrogen bonding interactions.					
33586190	0	85	with	delivery	12:19	arg1	V-amylose					26:34	V-amylose	26:34	V-amylose	26:34	Therapeutic delivery with V-amylose.					
33586190	8	86	theme	amylase	1545:1551	arg1	enzyme					1553:1558	the amylase enzyme	1541:1558	the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles	1541:1680	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	8	87	theme	drug	1770:1773	arg1	release					1742:1748	enzyme-sensitive controlled oral and colon-specific release	1690:1748	enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug	1690:1773	The activity of the amylase enzyme produced by salivary glands, pancreas, gastrointestinal tract, and gut microbiota on amylose-based drug delivery vehicles promote enzyme-sensitive controlled oral and colon-specific release of the encapsulated drug.					
33586190	9	88	theme	hydrophobic	1810:1820	arg1	core					1822:1825	hydrophobic core	1810:1825	hydrophobic core	1810:1825	The single helical V-amylose with hydrophobic core and hydrophilic periphery forms inclusion complexes that improve the absorption and permeation of drugs having a high clogP index.					
33586190	6	89	theme	amylose	1170:1176	arg1	polysaccharide					1178:1191	the parent amylose polysaccharide	1159:1191	the parent amylose polysaccharide	1159:1191	These structural features of V-amylose differentiate it from the parent amylose polysaccharide and enable the accommodation and nanoencapsulation of a wide range of therapeutics in the former.					
33586190	1	90	theme	helical	41:47	arg1	structure					49:57	The helical structure	37:57	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis	37:287	The helical structure of V-amylose offering a superior encapsulation affinity compared with the other polysaccharides, especially toward the amphiphilic or hydrophobic molecules; in addition to providing a higher resistance toward enzymatic hydrolysis support its applications as a potential drug delivery vehicle.					
33586190	7	91	theme	helical	1310:1316	arg1	structure					1318:1326	The tightly packed helical structure	1291:1326	The tightly packed helical structure of V-amylose	1291:1339	The tightly packed helical structure of V-amylose provides extraordinary resistance toward digestion by amylase compared with the linear polysaccharides, which supports the application of V-amylose as controlled drug release systems.					
33586190	5	92	contain	having	1060:1065	arg2	height					1075:1080	a pitch height	1067:1080	a pitch height of 7.91-8.17 Å	1067:1095	The V-amylose polysaccharide chains fold into left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å.					
33586190	5	92	contain	having	1060:1065	arg1	turn					1055:1058	each turn	1050:1058	each turn having a pitch height of 7.91-8.17 Å	1050:1095	The V-amylose polysaccharide chains fold into left-handed single helix comprising of six glucose units in each turn having a pitch height of 7.91-8.17 Å.					
33586190	10	93	theme	delivery	2052:2059	arg1	features					2010:2017	the distinguished features	1992:2017	the distinguished features of V-amylose	1992:2030	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	10	93	theme	delivery	2052:2059	arg1	system					2061:2066	an imminent drug delivery system	2035:2066	an imminent drug delivery system	2035:2066	The present commentary highlights the distinguished features of V-amylose as an imminent drug delivery system.					
33586190	4	94	theme	ideal	822:826	arg1	system					842:847	an ideal drug delivery system	819:847	an ideal drug delivery system	819:847	Besides, V-amylose presents characteristics of an ideal drug delivery system, such as biocompatibility, physiological benevolence, nonimmunogenicity, and biodegradability.					
34360826	2	0	theme	Cell	148:151	arg1	glycosylation					161:173	Cell surface glycosylation	148:173	Cell surface glycosylation	148:173	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	10	1	theme	new	1322:1324	arg1	tool					1326:1329	a new tool	1320:1329	a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens	1320:1484	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	8	2	attach	presented	974:982	arg1	region					952:957	the higher mass region	936:957	the higher mass region	936:957	In the higher mass region, these species presented with multiple N-acetyllactosamine repeating units.					
34360826	8	2	attach	presented	974:982	arg2	species					966:972	these species	960:972	these species	960:972	In the higher mass region, these species presented with multiple N-acetyllactosamine repeating units.					
34360826	10	3	theme	RBC	1289:1291	arg1	N-glycosylation					1293:1307	human RBC N-glycosylation	1283:1307	human RBC N-glycosylation	1283:1307	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	5	4	theme	RBC	650:652	arg1	N-glycans					654:662	RBC N-glycans	650:662	RBC N-glycans	650:662	This work aims at exploring the human RBC N-glycome by high-sensitivity MALDI-MS techniques to outline a fingerprint of RBC N-glycans.					
34360826	8	5	theme	mass	947:950	arg1	region					952:957	the higher mass region	936:957	the higher mass region	936:957	In the higher mass region, these species presented with multiple N-acetyllactosamine repeating units.					
34360826	7	6	theme	predominant	791:801	arg1	occurrence					803:812	the predominant occurrence	787:812	the predominant occurrence of neutral and sialylated complex N-glycans	787:856	Results showed the predominant occurrence of neutral and sialylated complex N-glycans with bisected N-acetylglucosamine and core- and/or antennary fucosylation.					
34360826	5	7	theme	N-glycans	654:662	arg1	fingerprint					635:645	a fingerprint	633:645	a fingerprint of RBC N-glycans	633:662	This work aims at exploring the human RBC N-glycome by high-sensitivity MALDI-MS techniques to outline a fingerprint of RBC N-glycans.					
34360826	1	8	theme	complex	54:60	arg1	modification					81:92	a complex post-translational modification	52:92	a complex post-translational modification that conveys functional diversity to glycoconjugates	52:145	Glycosylation is a complex post-translational modification that conveys functional diversity to glycoconjugates.					
34360826	1	8	theme	complex	54:60	arg1	Glycosylation					35:47	Glycosylation	35:47	Glycosylation	35:47	Glycosylation is a complex post-translational modification that conveys functional diversity to glycoconjugates.					
34360826	8	9	theme	higher	940:945	arg1	region					952:957	the higher mass region	936:957	the higher mass region	936:957	In the higher mass region, these species presented with multiple N-acetyllactosamine repeating units.					
34360826	9	10	theme	detected	1047:1054	arg1	glycoforms					1056:1065	the detected glycoforms	1043:1065	the detected glycoforms	1043:1065	Amongst the detected glycoforms, the presence of glycans bearing ABO(H) antigens allowed us to define a distinctive spectrum for each blood group.					
34360826	2	11	theme	biological	192:201	arg1	activities					203:212	several biological activities	184:212	several biological activities such as induction of the intracellular signaling pathway and pathogen recognition	184:294	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	2	11	theme	biological	192:201	arg1	recognition					284:294	pathogen recognition	275:294	pathogen recognition	275:294	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	2	11	theme	biological	192:201	arg1	induction					222:230	induction	222:230	induction of the intracellular signaling pathway	222:269	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	8	12	theme	repeating	1018:1026	arg1	units					1028:1032	multiple N-acetyllactosamine repeating units	989:1032	multiple N-acetyllactosamine repeating units	989:1032	In the higher mass region, these species presented with multiple N-acetyllactosamine repeating units.					
34360826	1	13	theme	post-translational	62:79	arg1	modification					81:92	a complex post-translational modification	52:92	a complex post-translational modification that conveys functional diversity to glycoconjugates	52:145	Glycosylation is a complex post-translational modification that conveys functional diversity to glycoconjugates.					
34360826	1	13	theme	post-translational	62:79	arg1	Glycosylation					35:47	Glycosylation	35:47	Glycosylation	35:47	Glycosylation is a complex post-translational modification that conveys functional diversity to glycoconjugates.					
34360826	10	14	theme	distinct	1358:1365	arg1	changes					1375:1381	distinct glycome changes	1358:1381	distinct glycome changes associated with disease conditions	1358:1416	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	10	15	theme	N-glycosylation	1293:1307	arg1	exploration					1268:1278	a comprehensive exploration	1252:1278	a comprehensive exploration of human RBC N-glycosylation	1252:1307	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	2	16	theme	several	184:190	arg1	activities					203:212	several biological activities	184:212	several biological activities such as induction of the intracellular signaling pathway and pathogen recognition	184:294	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	2	16	theme	several	184:190	arg1	recognition					284:294	pathogen recognition	275:294	pathogen recognition	275:294	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	2	16	theme	several	184:190	arg1	induction					222:230	induction	222:230	induction of the intracellular signaling pathway	222:269	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	3	17	theme	blood	301:305	arg1	RBC					313:315	RBC	313:315	RBC	313:315	Red blood cell (RBC) membrane N-glycans determine blood type and influence cell lifespan.					
34360826	3	17	theme	blood	301:305	arg1	cell					307:310	Red blood cell	297:310	Red blood cell (RBC) membrane N-glycans	297:335	Red blood cell (RBC) membrane N-glycans determine blood type and influence cell lifespan.					
34360826	10	18	theme	molecular	1451:1459	arg1	recognition					1461:1471	the molecular recognition	1447:1471	the molecular recognition of pathogens	1447:1484	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	3	19	theme	cell	307:310	arg1	N-glycans					327:335	Red blood cell (RBC) membrane N-glycans	297:335	Red blood cell (RBC) membrane N-glycans	297:335	Red blood cell (RBC) membrane N-glycans determine blood type and influence cell lifespan.					
34360826	2	20	theme	signaling	253:261	arg1	pathway					263:269	the intracellular signaling pathway	235:269	the intracellular signaling pathway	235:269	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	10	21	theme	glycome	1367:1373	arg1	changes					1375:1381	distinct glycome changes	1358:1381	distinct glycome changes associated with disease conditions	1358:1416	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	8	22	theme	multiple	989:996	arg1	units					1028:1032	multiple N-acetyllactosamine repeating units	989:1032	multiple N-acetyllactosamine repeating units	989:1032	In the higher mass region, these species presented with multiple N-acetyllactosamine repeating units.					
34360826	9	23	theme	blood	1169:1173	arg1	group					1175:1179	each blood group	1164:1179	each blood group	1164:1179	Amongst the detected glycoforms, the presence of glycans bearing ABO(H) antigens allowed us to define a distinctive spectrum for each blood group.					
34360826	10	24	theme	advanced	1202:1209	arg1	techniques					1220:1229	advanced glycomic techniques	1202:1229	advanced glycomic techniques	1202:1229	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	3	25	theme	Red	297:299	arg1	RBC					313:315	RBC	313:315	RBC	313:315	Red blood cell (RBC) membrane N-glycans determine blood type and influence cell lifespan.					
34360826	3	25	theme	Red	297:299	arg1	cell					307:310	Red blood cell	297:310	Red blood cell (RBC) membrane N-glycans	297:335	Red blood cell (RBC) membrane N-glycans determine blood type and influence cell lifespan.					
34360826	1	26	theme	functional	107:116	arg1	diversity					118:126	functional diversity	107:126	functional diversity	107:126	Glycosylation is a complex post-translational modification that conveys functional diversity to glycoconjugates.					
34360826	6	27	theme	blood	744:748	arg1	groups					750:755	different blood groups	734:755	different blood groups	734:755	To this purpose, the MALDI-TOF spectra of healthy subjects harboring different blood groups were acquired.					
34360826	0	28	theme	Erythrocytes	21:32	arg1	N-Glycomics					0:10	N-Glycomics	0:10	N-Glycomics of Human Erythrocytes.	0:33	N-Glycomics of Human Erythrocytes.					
34360826	10	29	theme	changes	1375:1381	arg1	detection					1345:1353	the early detection	1335:1353	the early detection of distinct glycome changes associated with disease conditions	1335:1416	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	6	30	theme	different	734:742	arg1	groups					750:755	different blood groups	734:755	different blood groups	734:755	To this purpose, the MALDI-TOF spectra of healthy subjects harboring different blood groups were acquired.					
34360826	0	31	theme	Human	15:19	arg1	Erythrocytes					21:32	Human Erythrocytes	15:32	Human Erythrocytes	15:32	N-Glycomics of Human Erythrocytes.					
34360826	7	32	theme	bisected	863:870	arg1	N-acetylglucosamine					872:890	bisected N-acetylglucosamine	863:890	bisected N-acetylglucosamine	863:890	Results showed the predominant occurrence of neutral and sialylated complex N-glycans with bisected N-acetylglucosamine and core- and/or antennary fucosylation.					
34360826	3	33	theme	membrane	318:325	arg1	N-glycans					327:335	Red blood cell (RBC) membrane N-glycans	297:335	Red blood cell (RBC) membrane N-glycans	297:335	Red blood cell (RBC) membrane N-glycans determine blood type and influence cell lifespan.					
34360826	10	34	theme	pathogens	1476:1484	arg1	recognition					1461:1471	the molecular recognition	1447:1471	the molecular recognition of pathogens	1447:1484	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	7	35	theme	antennary	909:917	arg1	fucosylation					919:930	antennary fucosylation	909:930	antennary fucosylation	909:930	Results showed the predominant occurrence of neutral and sialylated complex N-glycans with bisected N-acetylglucosamine and core- and/or antennary fucosylation.					
34360826	8	36	theme	N-acetyllactosamine	998:1016	arg1	units					1028:1032	multiple N-acetyllactosamine repeating units	989:1032	multiple N-acetyllactosamine repeating units	989:1032	In the higher mass region, these species presented with multiple N-acetyllactosamine repeating units.					
34360826	10	37	theme	first	1190:1194	arg1	time					1196:1199	the first time	1186:1199	the first time	1186:1199	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	6	38	theme	subjects	715:722	arg1	spectra					696:702	the MALDI-TOF spectra	682:702	the MALDI-TOF spectra of healthy subjects harboring different blood groups	682:755	To this purpose, the MALDI-TOF spectra of healthy subjects harboring different blood groups were acquired.					
34360826	9	39	theme	ABO	1100:1102	arg1	antigens					1107:1114	ABO(H) antigens	1100:1114	ABO(H) antigens	1100:1114	Amongst the detected glycoforms, the presence of glycans bearing ABO(H) antigens allowed us to define a distinctive spectrum for each blood group.					
34360826	7	40	theme	N-glycans	848:856	arg1	occurrence					803:812	the predominant occurrence	787:812	the predominant occurrence of neutral and sialylated complex N-glycans	787:856	Results showed the predominant occurrence of neutral and sialylated complex N-glycans with bisected N-acetylglucosamine and core- and/or antennary fucosylation.					
34360826	10	41	theme	human	1283:1287	arg1	N-glycosylation					1293:1307	human RBC N-glycosylation	1283:1307	human RBC N-glycosylation	1283:1307	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	2	42	theme	pathogen	275:282	arg1	recognition					284:294	pathogen recognition	275:294	pathogen recognition	275:294	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	5	43	theme	high-sensitivity	585:600	arg1	techniques					611:620	high-sensitivity MALDI-MS techniques	585:620	high-sensitivity MALDI-MS techniques	585:620	This work aims at exploring the human RBC N-glycome by high-sensitivity MALDI-MS techniques to outline a fingerprint of RBC N-glycans.					
34360826	10	44	theme	glycomic	1211:1218	arg1	techniques					1220:1229	advanced glycomic techniques	1202:1229	advanced glycomic techniques	1202:1229	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	3	45	theme	blood	347:351	arg1	type					353:356	blood type	347:356	blood type	347:356	Red blood cell (RBC) membrane N-glycans determine blood type and influence cell lifespan.					
34360826	5	46	theme	MALDI-MS	602:609	arg1	techniques					611:620	high-sensitivity MALDI-MS techniques	585:620	high-sensitivity MALDI-MS techniques	585:620	This work aims at exploring the human RBC N-glycome by high-sensitivity MALDI-MS techniques to outline a fingerprint of RBC N-glycans.					
34360826	9	47	theme	H	1104:1104	arg1	antigens					1107:1114	ABO(H) antigens	1100:1114	ABO(H) antigens	1100:1114	Amongst the detected glycoforms, the presence of glycans bearing ABO(H) antigens allowed us to define a distinctive spectrum for each blood group.					
34360826	7	48	theme	sialylated	829:838	arg1	N-glycans					848:856	neutral and sialylated complex N-glycans	817:856	neutral and sialylated complex N-glycans	817:856	Results showed the predominant occurrence of neutral and sialylated complex N-glycans with bisected N-acetylglucosamine and core- and/or antennary fucosylation.					
34360826	4	49	theme	RBC	466:468	arg1	proteins					479:486	RBC membrane proteins	466:486	RBC membrane proteins	466:486	Although several proteomic studies have been carried out, the glycosylation of RBC membrane proteins has not been systematically investigated.					
34360826	2	50	theme	pathway	263:269	arg1	induction					222:230	induction	222:230	induction of the intracellular signaling pathway	222:269	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	2	50	theme	pathway	263:269	arg1	recognition					284:294	pathogen recognition	275:294	pathogen recognition	275:294	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	4	51	theme	proteomic	404:412	arg1	studies					414:420	several proteomic studies	396:420	several proteomic studies	396:420	Although several proteomic studies have been carried out, the glycosylation of RBC membrane proteins has not been systematically investigated.					
34360826	6	52	theme	healthy	707:713	arg1	subjects					715:722	healthy subjects	707:722	healthy subjects harboring different blood groups	707:755	To this purpose, the MALDI-TOF spectra of healthy subjects harboring different blood groups were acquired.					
34360826	7	53	theme	complex	840:846	arg1	N-glycans					848:856	neutral and sialylated complex N-glycans	817:856	neutral and sialylated complex N-glycans	817:856	Results showed the predominant occurrence of neutral and sialylated complex N-glycans with bisected N-acetylglucosamine and core- and/or antennary fucosylation.					
34360826	9	54	theme	distinctive	1139:1149	arg1	spectrum					1151:1158	a distinctive spectrum	1137:1158	a distinctive spectrum for each blood group	1137:1179	Amongst the detected glycoforms, the presence of glycans bearing ABO(H) antigens allowed us to define a distinctive spectrum for each blood group.					
34360826	2	55	theme	intracellular	239:251	arg1	pathway					263:269	the intracellular signaling pathway	235:269	the intracellular signaling pathway	235:269	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	4	56	theme	several	396:402	arg1	studies					414:420	several proteomic studies	396:420	several proteomic studies	396:420	Although several proteomic studies have been carried out, the glycosylation of RBC membrane proteins has not been systematically investigated.					
34360826	7	57	theme	neutral	817:823	arg1	N-glycans					848:856	neutral and sialylated complex N-glycans	817:856	neutral and sialylated complex N-glycans	817:856	Results showed the predominant occurrence of neutral and sialylated complex N-glycans with bisected N-acetylglucosamine and core- and/or antennary fucosylation.					
34360826	7	58	gly	sialylated	829:838	arg1	N-glycans					848:856	neutral and sialylated complex N-glycans	817:856	neutral and sialylated complex N-glycans	817:856	Results showed the predominant occurrence of neutral and sialylated complex N-glycans with bisected N-acetylglucosamine and core- and/or antennary fucosylation.					
34360826	4	59	theme	proteins	479:486	arg1	glycosylation					449:461	the glycosylation	445:461	the glycosylation of RBC membrane proteins	445:486	Although several proteomic studies have been carried out, the glycosylation of RBC membrane proteins has not been systematically investigated.					
34360826	10	60	theme	early	1339:1343	arg1	detection					1345:1353	the early detection	1335:1353	the early detection of distinct glycome changes associated with disease conditions	1335:1416	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	5	61	theme	human	562:566	arg1	N-glycome					572:580	the human RBC N-glycome	558:580	the human RBC N-glycome by high-sensitivity MALDI-MS techniques to outline a fingerprint of RBC N-glycans	558:662	This work aims at exploring the human RBC N-glycome by high-sensitivity MALDI-MS techniques to outline a fingerprint of RBC N-glycans.					
34360826	10	62	theme	disease	1399:1405	arg1	conditions					1407:1416	disease conditions	1399:1416	disease conditions	1399:1416	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	3	63	theme	cell	372:375	arg1	lifespan					377:384	cell lifespan	372:384	cell lifespan	372:384	Red blood cell (RBC) membrane N-glycans determine blood type and influence cell lifespan.					
34360826	4	64	theme	membrane	470:477	arg1	proteins					479:486	RBC membrane proteins	466:486	RBC membrane proteins	466:486	Although several proteomic studies have been carried out, the glycosylation of RBC membrane proteins has not been systematically investigated.					
34360826	5	65	theme	RBC	568:570	arg1	N-glycome					572:580	the human RBC N-glycome	558:580	the human RBC N-glycome by high-sensitivity MALDI-MS techniques to outline a fingerprint of RBC N-glycans	558:662	This work aims at exploring the human RBC N-glycome by high-sensitivity MALDI-MS techniques to outline a fingerprint of RBC N-glycans.					
34360826	6	66	theme	MALDI-TOF	686:694	arg1	spectra					696:702	the MALDI-TOF spectra	682:702	the MALDI-TOF spectra of healthy subjects harboring different blood groups	682:755	To this purpose, the MALDI-TOF spectra of healthy subjects harboring different blood groups were acquired.					
34360826	2	67	theme	surface	153:159	arg1	glycosylation					161:173	Cell surface glycosylation	148:173	Cell surface glycosylation	148:173	Cell surface glycosylation mediates several biological activities such as induction of the intracellular signaling pathway and pathogen recognition.					
34360826	9	68	theme	glycans	1084:1090	arg1	presence					1072:1079	the presence	1068:1079	the presence of glycans bearing ABO(H) antigens	1068:1114	Amongst the detected glycoforms, the presence of glycans bearing ABO(H) antigens allowed us to define a distinctive spectrum for each blood group.					
34360826	10	69	theme	comprehensive	1254:1266	arg1	exploration					1268:1278	a comprehensive exploration	1252:1278	a comprehensive exploration of human RBC N-glycosylation	1252:1307	For the first time, advanced glycomic techniques have been applied to a comprehensive exploration of human RBC N-glycosylation, providing a new tool for the early detection of distinct glycome changes associated with disease conditions as well as for understanding the molecular recognition of pathogens.					
34360826	4	70	gly	glycosylation	449:461	arg1	proteins					479:486	RBC membrane proteins	466:486	RBC membrane proteins	466:486	Although several proteomic studies have been carried out, the glycosylation of RBC membrane proteins has not been systematically investigated.					
32544320	5	0	theme	alpha-1	795:801	arg1	AGP					822:824	AGP	822:824	AGP	822:824	The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.					
32544320	5	0	theme	alpha-1	795:801	arg1	glycoprotein					808:819	alpha-1 acid glycoprotein	795:819	alpha-1 acid glycoprotein (AGP)	795:825	The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.					
32544320	8	1	theme	human	1105:1109	arg1	AGP					1111:1113	human AGP	1105:1113	human AGP	1105:1113	Glycosylation analysis of human AGP revealed the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery.					
32544320	5	2	theme	bovine	741:746	arg1	fetuin					748:753	bovine fetuin	741:753	bovine fetuin	741:753	The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.					
32544320	1	3	theme	biological	151:160	arg1	processes					162:170	many biological processes	146:170	many biological processes	146:170	Protein glycosylation is involved in many biological processes and physiological functions.					
32544320	0	4	theme	Carbon	86:91	arg1	LC-MS/MS					99:106	Porous Graphitic Carbon (PGC) LC-MS/MS	69:106	Porous Graphitic Carbon (PGC) LC-MS/MS	69:106	Isomeric Separation of N-Glycopeptides Derived from Glycoproteins by Porous Graphitic Carbon (PGC) LC-MS/MS.					
32544320	5	5	theme	different	700:708	arg1	types					717:721	different glycan types	700:721	different glycan types	700:721	The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.					
32544320	6	6	theme	Comprehensive	841:853	arg1	separation					875:884	Comprehensive structural isomeric separation	841:884	Comprehensive structural isomeric separation of glycopeptides	841:901	Comprehensive structural isomeric separation of glycopeptides was observed by high-resolution MS and confirmed by MS/MS.					
32544320	8	7	theme	AGP	1111:1113	arg1	analysis					1093:1100	Glycosylation analysis	1079:1100	Glycosylation analysis of human AGP	1079:1113	Glycosylation analysis of human AGP revealed the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery.					
32544320	5	8	theme	glycan	710:715	arg1	types					717:721	different glycan types	700:721	different glycan types	700:721	The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.					
32544320	9	9	theme	reproducible	1273:1284	arg1	technique					1248:1256	This newly developed separation technique	1216:1256	This newly developed separation technique	1216:1256	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	9	9	theme	reproducible	1273:1284	arg1	method					1308:1313	a reproducible and useful analytical method	1271:1313	a reproducible and useful analytical method	1271:1313	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	0	10	theme	PGC	94:96	arg1	LC-MS/MS					99:106	Porous Graphitic Carbon (PGC) LC-MS/MS	69:106	Porous Graphitic Carbon (PGC) LC-MS/MS	69:106	Isomeric Separation of N-Glycopeptides Derived from Glycoproteins by Porous Graphitic Carbon (PGC) LC-MS/MS.					
32544320	3	11	gly	glycopeptide	410:421	arg2	glycopeptide					410:421	glycopeptide isomers	410:429	glycopeptide isomers	410:429	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	7	12	theme	glycopeptide	993:1004	arg1	isomers					1006:1012	the glycopeptide isomers	989:1012	the glycopeptide isomers	989:1012	The specific structures of the glycopeptide isomers were identified and confirmed through exoglycosidase digestions.					
32544320	3	13	theme	separation	511:520	arg1	temperature					522:532	the separation temperature	507:532	the separation temperature	507:532	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	4	14	theme	glycopeptides	613:625	arg1	separation					599:608	the isomeric separation	586:608	the isomeric separation of glycopeptides resulting from highly specific enzymatic digestions	586:677	These findings permitted the isomeric separation of glycopeptides resulting from highly specific enzymatic digestions.					
32544320	2	15	from	advances	220:227	arg1	methodologies					241:253	LC-MS/MS methodologies	232:253	LC-MS/MS methodologies	232:253	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	8	16	theme	potential	1128:1136	arg1	use					1138:1140	the potential use	1124:1140	the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery	1124:1213	Glycosylation analysis of human AGP revealed the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery.					
32544320	7	17	theme	isomers	1006:1012	arg1	structures					975:984	The specific structures	962:984	The specific structures of the glycopeptide isomers	962:1012	The specific structures of the glycopeptide isomers were identified and confirmed through exoglycosidase digestions.					
32544320	3	18	theme	mobile	546:551	arg1	phases					553:558	basic mobile phases	540:558	basic mobile phases	540:558	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	7	19	gly	glycopeptide	993:1004	arg2	glycopeptide					993:1004	glycopeptide	993:1004	glycopeptide	993:1004	The specific structures of the glycopeptide isomers were identified and confirmed through exoglycosidase digestions.					
32544320	3	20	from	separation	396:405	arg1	-LC					463:465	porous graphitic carbon (PGC)-LC	434:465	porous graphitic carbon (PGC)-LC	434:465	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	9	21	theme	useful	1290:1295	arg1	technique					1248:1256	This newly developed separation technique	1216:1256	This newly developed separation technique	1216:1256	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	9	21	theme	useful	1290:1295	arg1	method					1308:1313	a reproducible and useful analytical method	1271:1313	a reproducible and useful analytical method	1271:1313	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	2	22	theme	glycosylation	287:299	arg1	one					304:306	one	304:306	one	304:306	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	2	22	theme	glycosylation	287:299	arg1	profiling					260:268	the profiling	256:268	the profiling of site-specific glycosylation	256:299	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	2	22	theme	glycosylation	287:299	arg1	challenges					332:341	the major analytical challenges	311:341	the major analytical challenges of glycoprotein analysis	311:366	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	1	23	theme	physiological	176:188	arg1	functions					190:198	physiological functions	176:198	physiological functions	176:198	Protein glycosylation is involved in many biological processes and physiological functions.					
32544320	5	24	theme	acid	803:806	arg1	AGP					822:824	AGP	822:824	AGP	822:824	The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.					
32544320	5	24	theme	acid	803:806	arg1	glycoprotein					808:819	alpha-1 acid glycoprotein	795:819	alpha-1 acid glycoprotein (AGP)	795:825	The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.					
32544320	9	25	theme	analytical	1297:1306	arg1	technique					1248:1256	This newly developed separation technique	1216:1256	This newly developed separation technique	1216:1256	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	9	25	theme	analytical	1297:1306	arg1	method					1308:1313	a reproducible and useful analytical method	1271:1313	a reproducible and useful analytical method	1271:1313	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	2	26	theme	challenges	332:341	arg1	one					304:306	one	304:306	one	304:306	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	2	26	theme	challenges	332:341	arg1	profiling					260:268	the profiling	256:268	the profiling of site-specific glycosylation	256:299	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	2	26	theme	challenges	332:341	arg1	challenges					332:341	the major analytical challenges	311:341	the major analytical challenges of glycoprotein analysis	311:366	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	8	27	gly	glycoprotein	1169:1180	arg1	glycoprotein					1169:1180	extensive glycoprotein analysis	1159:1189	extensive glycoprotein analysis for biomarker discovery	1159:1213	Glycosylation analysis of human AGP revealed the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery.					
32544320	3	28	theme	graphitic	441:449	arg1	-LC					463:465	porous graphitic carbon (PGC)-LC	434:465	porous graphitic carbon (PGC)-LC	434:465	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	0	29	theme	Isomeric	0:7	arg1	Separation					9:18	Isomeric Separation	0:18	Isomeric Separation of N-Glycopeptides	0:37	Isomeric Separation of N-Glycopeptides Derived from Glycoproteins by Porous Graphitic Carbon (PGC) LC-MS/MS.					
32544320	4	30	gly	glycopeptides	613:625	arg2	glycopeptides					613:625	glycopeptides	613:625	glycopeptides resulting from highly specific enzymatic digestions	613:677	These findings permitted the isomeric separation of glycopeptides resulting from highly specific enzymatic digestions.					
32544320	2	31	theme	analytical	321:330	arg1	challenges					332:341	the major analytical challenges	311:341	the major analytical challenges of glycoprotein analysis	311:366	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	3	32	theme	carbon	451:456	arg1	-LC					463:465	porous graphitic carbon (PGC)-LC	434:465	porous graphitic carbon (PGC)-LC	434:465	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	0	33	theme	N-Glycopeptides	23:37	arg1	Separation					9:18	Isomeric Separation	0:18	Isomeric Separation of N-Glycopeptides	0:37	Isomeric Separation of N-Glycopeptides Derived from Glycoproteins by Porous Graphitic Carbon (PGC) LC-MS/MS.					
32544320	7	34	theme	specific	966:973	arg1	structures					975:984	The specific structures	962:984	The specific structures of the glycopeptide isomers	962:1012	The specific structures of the glycopeptide isomers were identified and confirmed through exoglycosidase digestions.					
32544320	1	35	gly	glycosylation	117:129	arg1	functions					190:198	physiological functions	176:198	physiological functions	176:198	Protein glycosylation is involved in many biological processes and physiological functions.					
32544320	1	35	gly	glycosylation	117:129	arg1	processes					162:170	many biological processes	146:170	many biological processes	146:170	Protein glycosylation is involved in many biological processes and physiological functions.					
32544320	8	36	theme	Glycosylation	1079:1091	arg1	analysis					1093:1100	Glycosylation analysis	1079:1100	Glycosylation analysis of human AGP	1079:1113	Glycosylation analysis of human AGP revealed the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery.					
32544320	2	37	theme	major	315:319	arg1	challenges					332:341	the major analytical challenges	311:341	the major analytical challenges of glycoprotein analysis	311:366	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	4	38	theme	isomeric	590:597	arg1	separation					599:608	the isomeric separation	586:608	the isomeric separation of glycopeptides resulting from highly specific enzymatic digestions	586:677	These findings permitted the isomeric separation of glycopeptides resulting from highly specific enzymatic digestions.					
32544320	8	39	theme	PGC-LC-MS	1145:1153	arg1	use					1138:1140	the potential use	1124:1140	the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery	1124:1213	Glycosylation analysis of human AGP revealed the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery.					
32544320	6	40	gly	glycopeptides	889:901	arg2	glycopeptides					889:901	glycopeptides	889:901	glycopeptides	889:901	Comprehensive structural isomeric separation of glycopeptides was observed by high-resolution MS and confirmed by MS/MS.					
32544320	8	41	theme	glycoprotein	1169:1180	arg1	analysis					1182:1189	extensive glycoprotein analysis	1159:1189	extensive glycoprotein analysis for biomarker discovery	1159:1213	Glycosylation analysis of human AGP revealed the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery.					
32544320	9	42	theme	site-specific	1324:1336	arg1	glycosylation					1347:1359	site-specific isomeric glycosylation	1324:1359	site-specific isomeric glycosylation	1324:1359	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	9	43	theme	developed	1227:1235	arg1	technique					1248:1256	This newly developed separation technique	1216:1256	This newly developed separation technique	1216:1256	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	9	43	theme	developed	1227:1235	arg1	method					1308:1313	a reproducible and useful analytical method	1271:1313	a reproducible and useful analytical method	1271:1313	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	3	44	theme	porous	434:439	arg1	-LC					463:465	porous graphitic carbon (PGC)-LC	434:465	porous graphitic carbon (PGC)-LC	434:465	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	8	45	theme	extensive	1159:1167	arg1	analysis					1182:1189	extensive glycoprotein analysis	1159:1189	extensive glycoprotein analysis for biomarker discovery	1159:1213	Glycosylation analysis of human AGP revealed the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery.					
32544320	9	46	theme	isomeric	1338:1345	arg1	glycosylation					1347:1359	site-specific isomeric glycosylation	1324:1359	site-specific isomeric glycosylation	1324:1359	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	3	47	theme	glycopeptide	410:421	arg1	isomers					423:429	glycopeptide isomers	410:429	glycopeptide isomers	410:429	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	6	48	theme	glycopeptides	889:901	arg1	separation					875:884	Comprehensive structural isomeric separation	841:884	Comprehensive structural isomeric separation of glycopeptides	841:901	Comprehensive structural isomeric separation of glycopeptides was observed by high-resolution MS and confirmed by MS/MS.					
32544320	2	49	theme	LC-MS/MS	232:239	arg1	methodologies					241:253	LC-MS/MS methodologies	232:253	LC-MS/MS methodologies	232:253	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	1	50	theme	Protein	109:115	arg1	glycosylation					117:129	Protein glycosylation	109:129	Protein glycosylation	109:129	Protein glycosylation is involved in many biological processes and physiological functions.					
32544320	3	51	theme	isomers	423:429	arg1	separation					396:405	the separation	392:405	the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC	392:465	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	2	52	theme	analysis	359:366	arg1	challenges					332:341	the major analytical challenges	311:341	the major analytical challenges of glycoprotein analysis	311:366	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	2	53	theme	recent	213:218	arg1	advances					220:227	the recent advances	209:227	the recent advances in LC-MS/MS methodologies	209:253	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	2	54	theme	site-specific	273:285	arg1	glycosylation					287:299	site-specific glycosylation	273:299	site-specific glycosylation	273:299	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	8	55	theme	biomarker	1195:1203	arg1	discovery					1205:1213	biomarker discovery	1195:1213	biomarker discovery	1195:1213	Glycosylation analysis of human AGP revealed the potential use of PGC-LC-MS for extensive glycoprotein analysis for biomarker discovery.					
32544320	2	56	theme	glycoprotein	346:357	arg1	analysis					359:366	glycoprotein analysis	346:366	glycoprotein analysis	346:366	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	5	57	theme	ribonuclease	775:786	arg1	B					788:788	ribonuclease B	775:788	ribonuclease B	775:788	The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.					
32544320	2	58	gly	glycoprotein	346:357	arg1	glycoprotein					346:357	glycoprotein analysis	346:366	glycoprotein analysis	346:366	Despite the recent advances in LC-MS/MS methodologies, the profiling of site-specific glycosylation is one of the major analytical challenges of glycoprotein analysis.					
32544320	6	59	theme	isomeric	866:873	arg1	separation					875:884	Comprehensive structural isomeric separation	841:884	Comprehensive structural isomeric separation of glycopeptides	841:901	Comprehensive structural isomeric separation of glycopeptides was observed by high-resolution MS and confirmed by MS/MS.					
32544320	5	60	gly	glycoprotein	808:819	arg1	AGP					822:824	AGP	822:824	AGP	822:824	The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.					
32544320	5	60	gly	glycoprotein	808:819	arg1	glycoprotein					808:819	alpha-1 acid glycoprotein	795:819	alpha-1 acid glycoprotein (AGP)	795:825	The selectivity for different glycan types was studied using bovine fetuin, asialofetuin, IgG, ribonuclease B, and alpha-1 acid glycoprotein (AGP) by PGC-LC-MS.					
32544320	9	61	theme	separation	1237:1246	arg1	technique					1248:1256	This newly developed separation technique	1216:1256	This newly developed separation technique	1216:1256	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	9	61	theme	separation	1237:1246	arg1	method					1308:1313	a reproducible and useful analytical method	1271:1313	a reproducible and useful analytical method	1271:1313	This newly developed separation technique was shown as a reproducible and useful analytical method to study site-specific isomeric glycosylation.					
32544320	3	62	theme	basic	540:544	arg1	phases					553:558	basic mobile phases	540:558	basic mobile phases	540:558	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	0	63	theme	Graphitic	76:84	arg1	LC-MS/MS					99:106	Porous Graphitic Carbon (PGC) LC-MS/MS	69:106	Porous Graphitic Carbon (PGC) LC-MS/MS	69:106	Isomeric Separation of N-Glycopeptides Derived from Glycoproteins by Porous Graphitic Carbon (PGC) LC-MS/MS.					
32544320	6	64	theme	structural	855:864	arg1	separation					875:884	Comprehensive structural isomeric separation	841:884	Comprehensive structural isomeric separation of glycopeptides	841:901	Comprehensive structural isomeric separation of glycopeptides was observed by high-resolution MS and confirmed by MS/MS.					
32544320	7	65	theme	exoglycosidase	1052:1065	arg1	digestions					1067:1076	exoglycosidase digestions	1052:1076	exoglycosidase digestions	1052:1076	The specific structures of the glycopeptide isomers were identified and confirmed through exoglycosidase digestions.					
32544320	6	66	theme	high-resolution	919:933	arg1	MS					935:936	high-resolution MS	919:936	high-resolution MS	919:936	Comprehensive structural isomeric separation of glycopeptides was observed by high-resolution MS and confirmed by MS/MS.					
32544320	3	67	theme	PGC	459:461	arg1	-LC					463:465	porous graphitic carbon (PGC)-LC	434:465	porous graphitic carbon (PGC)-LC	434:465	Herein, we report that the separation of glycopeptide isomers on porous graphitic carbon (PGC)-LC was significantly improved by elevating the separation temperature under basic mobile phases.					
32544320	4	68	theme	enzymatic	658:666	arg1	digestions					668:677	highly specific enzymatic digestions	642:677	highly specific enzymatic digestions	642:677	These findings permitted the isomeric separation of glycopeptides resulting from highly specific enzymatic digestions.					
32544320	0	69	theme	Porous	69:74	arg1	LC-MS/MS					99:106	Porous Graphitic Carbon (PGC) LC-MS/MS	69:106	Porous Graphitic Carbon (PGC) LC-MS/MS	69:106	Isomeric Separation of N-Glycopeptides Derived from Glycoproteins by Porous Graphitic Carbon (PGC) LC-MS/MS.					
32544320	1	70	theme	many	146:149	arg1	processes					162:170	many biological processes	146:170	many biological processes	146:170	Protein glycosylation is involved in many biological processes and physiological functions.					
32544320	4	71	theme	specific	649:656	arg1	digestions					668:677	highly specific enzymatic digestions	642:677	highly specific enzymatic digestions	642:677	These findings permitted the isomeric separation of glycopeptides resulting from highly specific enzymatic digestions.					
33585262	12	0	theme	E-gly-Hc	2065:2072	arg1	cap59					2084:2088	E-gly-Hc coated Cn cap59	2065:2088	E-gly-Hc coated Cn cap59	2065:2088	C-gly-Hc and E-gly-Hc coated Cn cap59 were also able to kill larvae of Galleria mellonella.					
33585262	10	1	theme	cross-reacting	1913:1926	arg1	antibodies					1928:1937	cross-reacting antibodies	1913:1937	cross-reacting antibodies	1913:1937	In turn, sera from histoplasmosis patients recognized Cn glycans, suggesting immunogenicity and the presence of cross-reacting antibodies.					
33585262	7	2	theme	proteins	1475:1482	arg1	Mapping					1429:1435	Mapping	1429:1435	Mapping of recognized Cn GXM synthesis/export proteins	1429:1482	Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database.					
33585262	4	3	theme	fungus	938:943	arg1	Hc					969:970	Hc	969:970	Hc	969:970	Previously, we demonstrated that the fungus Histoplasma capsulatum (Hc) incorporates, surface/secreted GXM of Cn and the surface accumulation of the polysaccharide enhances Hc virulence in vitro and in vivo.					
33585262	4	3	theme	fungus	938:943	arg1	capsulatum					957:966	the fungus Histoplasma capsulatum	934:966	the fungus Histoplasma capsulatum (Hc)	934:971	Previously, we demonstrated that the fungus Histoplasma capsulatum (Hc) incorporates, surface/secreted GXM of Cn and the surface accumulation of the polysaccharide enhances Hc virulence in vitro and in vivo.					
33585262	6	4	contain	had	1375:1377	arg2	xylose					1382:1387	no xylose	1379:1387	no xylose	1379:1387	These C-gly-Hc are readily incorporated on the surface of acapsular Cn cap59; however, in contrast to Cn GXM, C-gly-Hc had no xylose and glucuronic acid in its composition.					
33585262	6	4	contain	had	1375:1377	arg1	C-gly-Hc					1366:1373	C-gly-Hc	1366:1373	C-gly-Hc	1366:1373	These C-gly-Hc are readily incorporated on the surface of acapsular Cn cap59; however, in contrast to Cn GXM, C-gly-Hc had no xylose and glucuronic acid in its composition.					
33585262	6	4	contain	had	1375:1377	arg2	acid					1404:1407	glucuronic acid	1393:1407	glucuronic acid	1393:1407	These C-gly-Hc are readily incorporated on the surface of acapsular Cn cap59; however, in contrast to Cn GXM, C-gly-Hc had no xylose and glucuronic acid in its composition.					
33585262	8	5	from	Evaluation	1540:1549	arg1	strains					1581:1587	strains	1581:1587	strains of distinct monophyletic clades	1581:1619	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	2	6	theme	Additional	265:274	arg1	structures					285:294	Additional external structures	265:294	Additional external structures	265:294	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	8	7	theme	molecular	1700:1708	arg1	dimensions					1710:1719	their molecular dimensions	1694:1719	their molecular dimensions	1694:1719	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	7	8	theme	GXM	1454:1456	arg1	proteins					1475:1482	recognized Cn GXM synthesis/export proteins	1440:1482	recognized Cn GXM synthesis/export proteins	1440:1482	Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database.					
33585262	3	9	theme	GXM-specific	747:758	arg1	mAbs					783:786	mAbs	783:786	mAbs	783:786	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	3	9	theme	GXM-specific	747:758	arg1	antibodies					771:780	GXM-specific monoclonal antibodies	747:780	GXM-specific monoclonal antibodies (mAbs)	747:787	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	1	10	theme	fungal	196:201	arg1	kingdom					203:209	the fungal kingdom	192:209	the fungal kingdom	192:209	The cell wall is a ubiquitous structure in the fungal kingdom, with some features varying depending on the species.					
33585262	7	11	theme	recognized	1440:1449	arg1	proteins					1475:1482	recognized Cn GXM synthesis/export proteins	1440:1482	recognized Cn GXM synthesis/export proteins	1440:1482	Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database.					
33585262	3	12	theme	distant	576:582	arg1	species					584:590	even more evolutionarily distant species	551:590	even more evolutionarily distant species	551:590	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	3	12	theme	distant	576:582	arg1	mucoides					629:636	mucoides	629:636	mucoides	629:636	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	2	13	theme	anti-phagocytic	444:458	arg1	properties					482:491	anti-phagocytic and anti-inflammatory properties	444:491	anti-phagocytic and anti-inflammatory properties	444:491	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	4	14	theme	surface/secreted	987:1002	arg1	GXM					1004:1006	surface/secreted GXM	987:1006	surface/secreted GXM of Cn	987:1012	Previously, we demonstrated that the fungus Histoplasma capsulatum (Hc) incorporates, surface/secreted GXM of Cn and the surface accumulation of the polysaccharide enhances Hc virulence in vitro and in vivo.					
33585262	7	15	attach	presence	1498:1505	arg1	database					1530:1537	the Hc database	1523:1537	the Hc database	1523:1537	Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database.					
33585262	7	15	attach	presence	1498:1505	arg2	orthologs					1510:1518	orthologs	1510:1518	orthologs	1510:1518	Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database.					
33585262	12	16	theme	mellonella	2132:2141	arg1	larvae					2113:2118	larvae	2113:2118	larvae of Galleria mellonella	2113:2141	C-gly-Hc and E-gly-Hc coated Cn cap59 were also able to kill larvae of Galleria mellonella.					
33585262	8	17	from	strains	1581:1587	arg1	Evaluation					1540:1549	Evaluation	1540:1549	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades	1540:1619	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	8	17	from	strains	1581:1587	arg1	Hc					1573:1574	Hc	1573:1574	Hc from strains of distinct monophyletic clades	1573:1619	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	13	18	theme	GXM-like	2150:2157	arg1	glycans					2162:2168	These GXM-like Hc glycans	2144:2168	These GXM-like Hc glycans	2144:2168	These GXM-like Hc glycans, as well as those produced by other pathogenic fungi, may also be important during host-pathogen interactions, and factors associated with their regulation are potentially important targets for the management of histoplasmosis.					
33585262	6	19	theme	cap59	1327:1331	arg1	surface					1303:1309	the surface	1299:1309	the surface of acapsular Cn cap59	1299:1331	These C-gly-Hc are readily incorporated on the surface of acapsular Cn cap59; however, in contrast to Cn GXM, C-gly-Hc had no xylose and glucuronic acid in its composition.					
33585262	11	20	theme	coated	1976:1981	arg1	cap59					1986:1990	E-gly-Hc coated Cn cap59	1967:1990	E-gly-Hc coated Cn cap59	1967:1990	Additionally, C-gly-Hc and E-gly-Hc coated Cn cap59 were more resistant to phagocytosis and macrophage killing.					
33585262	1	21	theme	cell	153:156	arg1	wall					158:161	The cell wall	149:161	The cell wall	149:161	The cell wall is a ubiquitous structure in the fungal kingdom, with some features varying depending on the species.					
33585262	1	21	theme	cell	153:156	arg1	structure					179:187	a ubiquitous structure	166:187	a ubiquitous structure in the fungal kingdom	166:209	The cell wall is a ubiquitous structure in the fungal kingdom, with some features varying depending on the species.					
33585262	6	22	theme	acapsular	1314:1322	arg1	cap59					1327:1331	acapsular Cn cap59	1314:1331	acapsular Cn cap59	1314:1331	These C-gly-Hc are readily incorporated on the surface of acapsular Cn cap59; however, in contrast to Cn GXM, C-gly-Hc had no xylose and glucuronic acid in its composition.					
33585262	2	23	theme	external	276:283	arg1	structures					285:294	Additional external structures	265:294	Additional external structures	265:294	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	4	24	theme	surface	1022:1028	arg1	accumulation					1030:1041	the surface accumulation	1018:1041	the surface accumulation of the polysaccharide	1018:1063	Previously, we demonstrated that the fungus Histoplasma capsulatum (Hc) incorporates, surface/secreted GXM of Cn and the surface accumulation of the polysaccharide enhances Hc virulence in vitro and in vivo.					
33585262	12	25	theme	Cn	2081:2082	arg1	cap59					2084:2088	E-gly-Hc coated Cn cap59	2065:2088	E-gly-Hc coated Cn cap59	2065:2088	C-gly-Hc and E-gly-Hc coated Cn cap59 were also able to kill larvae of Galleria mellonella.					
33585262	3	26	theme	similar	829:835	arg1	composition					837:847	similar composition	829:847	similar composition	829:847	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	5	27	theme	Hc	1155:1156	arg1	ability					1144:1150	the ability	1140:1150	the ability of Hc to produce cellular-attached (C-gly-Hc) and secreted (E-gly) glycans with reactivity to GXM mAbs	1140:1253	In this work, we characterized the ability of Hc to produce cellular-attached (C-gly-Hc) and secreted (E-gly) glycans with reactivity to GXM mAbs.					
33585262	1	28	theme	ubiquitous	168:177	arg1	wall					158:161	The cell wall	149:161	The cell wall	149:161	The cell wall is a ubiquitous structure in the fungal kingdom, with some features varying depending on the species.					
33585262	1	28	theme	ubiquitous	168:177	arg1	structure					179:187	a ubiquitous structure	166:187	a ubiquitous structure in the fungal kingdom	166:209	The cell wall is a ubiquitous structure in the fungal kingdom, with some features varying depending on the species.					
33585262	3	29	theme	cryptococcal	883:894	arg1	GXM					896:898	cryptococcal GXM	883:898	cryptococcal GXM	883:898	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	1	30	from	structure	179:187	arg1	kingdom					203:209	the fungal kingdom	192:209	the fungal kingdom	192:209	The cell wall is a ubiquitous structure in the fungal kingdom, with some features varying depending on the species.					
33585262	0	31	theme	Cryptococcus	104:115	arg1	neoformans					117:126	Cryptococcus neoformans	104:126	Cryptococcus neoformans	104:126	Histoplasma capsulatum Glycans From Distinct Genotypes Share Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan.					
33585262	9	32	theme	patients	1791:1798	arg1	sera					1768:1771	sera	1768:1771	sera of cryptococcosis patients	1768:1798	These C-gly-Hc and E-gly-Hc also reacted with sera of cryptococcosis patients.					
33585262	3	33	contain	have	824:827	arg1	polysaccharides					808:822	these complex polysaccharides	794:822	these complex polysaccharides	794:822	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	3	33	contain	have	824:827	arg2	properties					869:878	anti-phagocytic properties	853:878	anti-phagocytic properties	853:878	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	3	33	contain	have	824:827	arg2	composition					837:847	similar composition	829:847	similar composition	829:847	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	2	34	theme	anti-inflammatory	464:480	arg1	properties					482:491	anti-phagocytic and anti-inflammatory properties	444:491	anti-phagocytic and anti-inflammatory properties	444:491	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	2	35	theme	virulence	375:383	arg1	factor					385:390	its major virulence factor	365:390	its major virulence factor	365:390	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	2	35	theme	virulence	375:383	arg1	neoformans					348:357	Cryptococcus neoformans	335:357	Cryptococcus neoformans (Cn)	335:362	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	0	36	theme	Histoplasma	0:10	arg1	capsulatum					12:21	Histoplasma capsulatum	0:21	Histoplasma capsulatum Glycans From Distinct Genotypes	0:53	Histoplasma capsulatum Glycans From Distinct Genotypes Share Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan.					
33585262	5	37	theme	secreted	1202:1209	arg1	glycans					1219:1225	cellular-attached (C-gly-Hc) and secreted (E-gly) glycans	1169:1225	cellular-attached (C-gly-Hc) and secreted (E-gly) glycans	1169:1225	In this work, we characterized the ability of Hc to produce cellular-attached (C-gly-Hc) and secreted (E-gly) glycans with reactivity to GXM mAbs.					
33585262	5	38	dep	cellular-attached	1169:1185	arg1	C-gly-Hc					1188:1195	C-gly-Hc	1188:1195	C-gly-Hc	1188:1195	In this work, we characterized the ability of Hc to produce cellular-attached (C-gly-Hc) and secreted (E-gly) glycans with reactivity to GXM mAbs.					
33585262	4	39	theme	polysaccharide	1050:1063	arg1	accumulation					1030:1041	the surface accumulation	1018:1041	the surface accumulation of the polysaccharide	1018:1063	Previously, we demonstrated that the fungus Histoplasma capsulatum (Hc) incorporates, surface/secreted GXM of Cn and the surface accumulation of the polysaccharide enhances Hc virulence in vitro and in vivo.					
33585262	8	40	theme	C-gly	1554:1558	arg1	Evaluation					1540:1549	Evaluation	1540:1549	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades	1540:1619	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	0	41	theme	Distinct	36:43	arg1	Genotypes					45:53	Distinct Genotypes	36:53	Distinct Genotypes	36:53	Histoplasma capsulatum Glycans From Distinct Genotypes Share Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan.					
33585262	13	42	theme	other	2200:2204	arg1	fungi					2217:2221	other pathogenic fungi	2200:2221	other pathogenic fungi	2200:2221	These GXM-like Hc glycans, as well as those produced by other pathogenic fungi, may also be important during host-pathogen interactions, and factors associated with their regulation are potentially important targets for the management of histoplasmosis.					
33585262	4	43	theme	Hc	1074:1075	arg1	virulence					1077:1085	Hc virulence	1074:1085	Hc virulence	1074:1085	Previously, we demonstrated that the fungus Histoplasma capsulatum (Hc) incorporates, surface/secreted GXM of Cn and the surface accumulation of the polysaccharide enhances Hc virulence in vitro and in vivo.					
33585262	8	44	theme	E-gly	1564:1568	arg1	Evaluation					1540:1549	Evaluation	1540:1549	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades	1540:1619	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	10	45	theme	Cn	1855:1856	arg1	glycans					1858:1864	Cn glycans	1855:1864	Cn glycans	1855:1864	In turn, sera from histoplasmosis patients recognized Cn glycans, suggesting immunogenicity and the presence of cross-reacting antibodies.					
33585262	7	46	theme	orthologs	1510:1518	arg1	presence					1498:1505	the presence	1494:1505	the presence of orthologs in the Hc database	1494:1537	Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database.					
33585262	8	47	theme	Hc	1573:1574	arg1	Evaluation					1540:1549	Evaluation	1540:1549	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades	1540:1619	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	2	48	theme	Cryptococcus	335:346	arg1	neoformans					348:357	Cryptococcus neoformans	335:357	Cryptococcus neoformans (Cn)	335:362	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	2	48	theme	Cryptococcus	335:346	arg1	factor					385:390	its major virulence factor	365:390	its major virulence factor	365:390	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	2	48	theme	Cryptococcus	335:346	arg1	Cn					360:361	Cn	360:361	Cn	360:361	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	5	49	theme	GXM	1246:1248	arg1	mAbs					1250:1253	GXM mAbs	1246:1253	GXM mAbs	1246:1253	In this work, we characterized the ability of Hc to produce cellular-attached (C-gly-Hc) and secreted (E-gly) glycans with reactivity to GXM mAbs.					
33585262	13	50	theme	host-pathogen	2253:2265	arg1	interactions					2267:2278	host-pathogen interactions	2253:2278	host-pathogen interactions	2253:2278	These GXM-like Hc glycans, as well as those produced by other pathogenic fungi, may also be important during host-pathogen interactions, and factors associated with their regulation are potentially important targets for the management of histoplasmosis.					
33585262	3	51	theme	other	520:524	arg1	species					539:545	other cryptococcal species	520:545	other cryptococcal species	520:545	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	3	51	theme	other	520:524	arg1	mucoides					629:636	mucoides	629:636	mucoides	629:636	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	6	52	theme	glucuronic	1393:1402	arg1	acid					1404:1407	glucuronic acid	1393:1407	glucuronic acid	1393:1407	These C-gly-Hc are readily incorporated on the surface of acapsular Cn cap59; however, in contrast to Cn GXM, C-gly-Hc had no xylose and glucuronic acid in its composition.					
33585262	8	53	theme	distinct	1592:1599	arg1	clades					1614:1619	distinct monophyletic clades	1592:1619	distinct monophyletic clades	1592:1619	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	10	54	theme	antibodies	1928:1937	arg1	immunogenicity					1878:1891	immunogenicity	1878:1891	immunogenicity	1878:1891	In turn, sera from histoplasmosis patients recognized Cn glycans, suggesting immunogenicity and the presence of cross-reacting antibodies.					
33585262	10	54	theme	antibodies	1928:1937	arg1	presence					1901:1908	the presence	1897:1908	the presence of cross-reacting antibodies	1897:1937	In turn, sera from histoplasmosis patients recognized Cn glycans, suggesting immunogenicity and the presence of cross-reacting antibodies.					
33585262	7	55	theme	synthesis/export	1458:1473	arg1	proteins					1475:1482	recognized Cn GXM synthesis/export proteins	1440:1482	recognized Cn GXM synthesis/export proteins	1440:1482	Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database.					
33585262	8	56	theme	clades	1614:1619	arg1	strains					1581:1587	strains	1581:1587	strains of distinct monophyletic clades	1581:1619	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	3	57	theme	serological	721:731	arg1	reactivity					733:742	serological reactivity	721:742	serological reactivity to GXM-specific monoclonal antibodies (mAbs)	721:787	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	11	58	theme	macrophage	2032:2041	arg1	killing					2043:2049	macrophage killing	2032:2049	macrophage killing	2032:2049	Additionally, C-gly-Hc and E-gly-Hc coated Cn cap59 were more resistant to phagocytosis and macrophage killing.					
33585262	7	59	theme	Cn	1451:1452	arg1	proteins					1475:1482	recognized Cn GXM synthesis/export proteins	1440:1482	recognized Cn GXM synthesis/export proteins	1440:1482	Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database.					
33585262	8	60	theme	serological	1628:1638	arg1	reactivity					1640:1649	serological reactivity	1628:1649	serological reactivity to GXM mAbs	1628:1661	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	13	61	theme	important	2342:2350	arg1	targets					2352:2358	potentially important targets	2330:2358	potentially important targets for the management of histoplasmosis	2330:2395	These GXM-like Hc glycans, as well as those produced by other pathogenic fungi, may also be important during host-pathogen interactions, and factors associated with their regulation are potentially important targets for the management of histoplasmosis.					
33585262	13	61	theme	important	2342:2350	arg1	factors					2285:2291	factors	2285:2291	factors associated with their regulation	2285:2324	These GXM-like Hc glycans, as well as those produced by other pathogenic fungi, may also be important during host-pathogen interactions, and factors associated with their regulation are potentially important targets for the management of histoplasmosis.					
33585262	3	62	theme	monoclonal	760:769	arg1	mAbs					783:786	mAbs	783:786	mAbs	783:786	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	3	62	theme	monoclonal	760:769	arg1	antibodies					771:780	GXM-specific monoclonal antibodies	747:780	GXM-specific monoclonal antibodies (mAbs)	747:787	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	11	63	theme	Cn	1983:1984	arg1	cap59					1986:1990	E-gly-Hc coated Cn cap59	1967:1990	E-gly-Hc coated Cn cap59	1967:1990	Additionally, C-gly-Hc and E-gly-Hc coated Cn cap59 were more resistant to phagocytosis and macrophage killing.					
33585262	12	64	theme	Galleria	2123:2130	arg1	mellonella					2132:2141	Galleria mellonella	2123:2141	Galleria mellonella	2123:2141	C-gly-Hc and E-gly-Hc coated Cn cap59 were also able to kill larvae of Galleria mellonella.					
33585262	13	65	theme	Hc	2159:2160	arg1	glycans					2162:2168	These GXM-like Hc glycans	2144:2168	These GXM-like Hc glycans	2144:2168	These GXM-like Hc glycans, as well as those produced by other pathogenic fungi, may also be important during host-pathogen interactions, and factors associated with their regulation are potentially important targets for the management of histoplasmosis.					
33585262	11	66	theme	E-gly-Hc	1967:1974	arg1	cap59					1986:1990	E-gly-Hc coated Cn cap59	1967:1990	E-gly-Hc coated Cn cap59	1967:1990	Additionally, C-gly-Hc and E-gly-Hc coated Cn cap59 were more resistant to phagocytosis and macrophage killing.					
33585262	4	67	theme	Histoplasma	945:955	arg1	Hc					969:970	Hc	969:970	Hc	969:970	Previously, we demonstrated that the fungus Histoplasma capsulatum (Hc) incorporates, surface/secreted GXM of Cn and the surface accumulation of the polysaccharide enhances Hc virulence in vitro and in vivo.					
33585262	4	67	theme	Histoplasma	945:955	arg1	capsulatum					957:966	the fungus Histoplasma capsulatum	934:966	the fungus Histoplasma capsulatum (Hc)	934:971	Previously, we demonstrated that the fungus Histoplasma capsulatum (Hc) incorporates, surface/secreted GXM of Cn and the surface accumulation of the polysaccharide enhances Hc virulence in vitro and in vivo.					
33585262	3	68	theme	complex	800:806	arg1	polysaccharides					808:822	these complex polysaccharides	794:822	these complex polysaccharides	794:822	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	6	69	theme	Cn	1324:1325	arg1	cap59					1327:1331	acapsular Cn cap59	1314:1331	acapsular Cn cap59	1314:1331	These C-gly-Hc are readily incorporated on the surface of acapsular Cn cap59; however, in contrast to Cn GXM, C-gly-Hc had no xylose and glucuronic acid in its composition.					
33585262	3	70	theme	anti-phagocytic	853:867	arg1	properties					869:878	anti-phagocytic properties	853:878	anti-phagocytic properties	853:878	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	0	71	theme	Serological	76:86	arg1	Similarities					88:99	Structural and Serological Similarities	61:99	Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan	61:146	Histoplasma capsulatum Glycans From Distinct Genotypes Share Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan.					
33585262	10	72	theme	histoplasmosis	1820:1833	arg1	patients					1835:1842	histoplasmosis patients	1820:1842	histoplasmosis patients	1820:1842	In turn, sera from histoplasmosis patients recognized Cn glycans, suggesting immunogenicity and the presence of cross-reacting antibodies.					
33585262	12	73	theme	coated	2074:2079	arg1	cap59					2084:2088	E-gly-Hc coated Cn cap59	2065:2088	E-gly-Hc coated Cn cap59	2065:2088	C-gly-Hc and E-gly-Hc coated Cn cap59 were also able to kill larvae of Galleria mellonella.					
33585262	1	74	with	structure	179:187	arg1	features					222:229	some features	217:229	some features varying depending on the species	217:262	The cell wall is a ubiquitous structure in the fungal kingdom, with some features varying depending on the species.					
33585262	5	75	dep	secreted	1202:1209	arg1	E-gly					1212:1216	E-gly	1212:1216	E-gly	1212:1216	In this work, we characterized the ability of Hc to produce cellular-attached (C-gly-Hc) and secreted (E-gly) glycans with reactivity to GXM mAbs.					
33585262	2	76	dep	present	303:309	arg1	such					312:315	such	312:315	such	312:315	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	5	77	theme	cellular-attached	1169:1185	arg1	glycans					1219:1225	cellular-attached (C-gly-Hc) and secreted (E-gly) glycans	1169:1225	cellular-attached (C-gly-Hc) and secreted (E-gly) glycans	1169:1225	In this work, we characterized the ability of Hc to produce cellular-attached (C-gly-Hc) and secreted (E-gly) glycans with reactivity to GXM mAbs.					
33585262	2	78	with	capsule	324:330	arg1	properties					482:491	anti-phagocytic and anti-inflammatory properties	444:491	anti-phagocytic and anti-inflammatory properties	444:491	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	9	79	theme	cryptococcosis	1776:1789	arg1	patients					1791:1798	cryptococcosis patients	1776:1798	cryptococcosis patients	1776:1798	These C-gly-Hc and E-gly-Hc also reacted with sera of cryptococcosis patients.					
33585262	6	80	theme	Cn	1358:1359	arg1	GXM					1361:1363	Cn GXM	1358:1363	Cn GXM	1358:1363	These C-gly-Hc are readily incorporated on the surface of acapsular Cn cap59; however, in contrast to Cn GXM, C-gly-Hc had no xylose and glucuronic acid in its composition.					
33585262	0	81	dep	neoformans	117:126	arg1	Glucuronoxylomannan					128:146	Glucuronoxylomannan	128:146	Glucuronoxylomannan	128:146	Histoplasma capsulatum Glycans From Distinct Genotypes Share Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan.					
33585262	4	82	theme	Cn	1011:1012	arg1	GXM					1004:1006	surface/secreted GXM	987:1006	surface/secreted GXM of Cn	987:1012	Previously, we demonstrated that the fungus Histoplasma capsulatum (Hc) incorporates, surface/secreted GXM of Cn and the surface accumulation of the polysaccharide enhances Hc virulence in vitro and in vivo.					
33585262	0	83	from	Genotypes	45:53	arg1	Glycans					23:29	Histoplasma capsulatum Glycans	0:29	Histoplasma capsulatum Glycans From Distinct Genotypes	0:53	Histoplasma capsulatum Glycans From Distinct Genotypes Share Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan.					
33585262	0	84	theme	capsulatum	12:21	arg1	Glycans					23:29	Histoplasma capsulatum Glycans	0:29	Histoplasma capsulatum Glycans From Distinct Genotypes	0:53	Histoplasma capsulatum Glycans From Distinct Genotypes Share Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan.					
33585262	8	85	from	differences	1679:1689	arg1	dimensions					1710:1719	their molecular dimensions	1694:1719	their molecular dimensions	1694:1719	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	2	86	theme	major	369:373	arg1	factor					385:390	its major virulence factor	365:390	its major virulence factor	365:390	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	2	86	theme	major	369:373	arg1	neoformans					348:357	Cryptococcus neoformans	335:357	Cryptococcus neoformans (Cn)	335:362	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	10	87	from	patients	1835:1842	arg1	sera					1810:1813	sera	1810:1813	sera from histoplasmosis patients	1810:1842	In turn, sera from histoplasmosis patients recognized Cn glycans, suggesting immunogenicity and the presence of cross-reacting antibodies.					
33585262	0	88	theme	Structural	61:70	arg1	Similarities					88:99	Structural and Serological Similarities	61:99	Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan	61:146	Histoplasma capsulatum Glycans From Distinct Genotypes Share Structural and Serological Similarities to Cryptococcus neoformans Glucuronoxylomannan.					
33585262	13	89	theme	pathogenic	2206:2215	arg1	fungi					2217:2221	other pathogenic fungi	2200:2221	other pathogenic fungi	2200:2221	These GXM-like Hc glycans, as well as those produced by other pathogenic fungi, may also be important during host-pathogen interactions, and factors associated with their regulation are potentially important targets for the management of histoplasmosis.					
33585262	7	90	theme	Hc	1527:1528	arg1	database					1530:1537	the Hc database	1523:1537	the Hc database	1523:1537	Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database.					
33585262	3	91	theme	GXM-like	685:692	arg1	polysaccharides					694:708	GXM-like polysaccharides	685:708	GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs)	685:787	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	2	92	theme	neoformans	348:357	arg1	capsule					324:330	the capsule	320:330	the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties	320:491	Additional external structures can be present, such as the capsule of Cryptococcus neoformans (Cn), its major virulence factor, mainly composed of glucuronoxylomannan (GXM), with anti-phagocytic and anti-inflammatory properties.					
33585262	8	93	theme	GXM	1654:1656	arg1	mAbs					1658:1661	GXM mAbs	1654:1661	GXM mAbs	1654:1661	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	7	94	from	presence	1498:1505	arg1	database					1530:1537	the Hc database	1523:1537	the Hc database	1523:1537	Mapping of recognized Cn GXM synthesis/export proteins confirmed the presence of orthologs in the Hc database.					
33585262	13	95	theme	histoplasmosis	2382:2395	arg1	management					2368:2377	the management	2364:2377	the management of histoplasmosis	2364:2395	These GXM-like Hc glycans, as well as those produced by other pathogenic fungi, may also be important during host-pathogen interactions, and factors associated with their regulation are potentially important targets for the management of histoplasmosis.					
33585262	8	96	theme	monophyletic	1601:1612	arg1	clades					1614:1619	distinct monophyletic clades	1592:1619	distinct monophyletic clades	1592:1619	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33585262	3	97	theme	cryptococcal	526:537	arg1	species					539:545	other cryptococcal species	520:545	other cryptococcal species	520:545	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	3	97	theme	cryptococcal	526:537	arg1	mucoides					629:636	mucoides	629:636	mucoides	629:636	The literature shows that other cryptococcal species and even more evolutionarily distant species, such as the Trichosporon asahii, T. mucoides, and Paracoccidioides brasiliensis can produce GXM-like polysaccharides displaying serological reactivity to GXM-specific monoclonal antibodies (mAbs), and these complex polysaccharides have similar composition and anti-phagocytic properties to cryptococcal GXM.					
33585262	8	98	theme	slight	1672:1677	arg1	differences					1679:1689	slight differences	1672:1689	slight differences in their molecular dimensions	1672:1719	Evaluation of C-gly and E-gly of Hc from strains of distinct monophyletic clades showed serological reactivity to GXM mAbs, despite slight differences in their molecular dimensions.					
33483058	6	0	theme	sulfate	992:998	arg1	groups					1000:1005	sulfate groups	992:1005	sulfate groups	992:1005	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	1	1	theme	diseases	155:162	arg1	treatments					141:150	the treatments	137:150	the treatments of diseases triggered through viral infections	137:197	Limited options for the treatments of diseases triggered through viral infections revealed the quest for novel antiviral drugs.					
33483058	8	2	theme	antiviral	1226:1234	arg1	activities					1236:1245	antiviral activities	1226:1245	antiviral activities	1226:1245	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	6	3	theme	xylopyranosyl	1037:1049	arg1	O-3					1010:1012	O-3	1010:1012	O-3	1010:1012	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	3	theme	xylopyranosyl	1037:1049	arg1	positions					1024:1032	O-2,3 positions	1018:1032	O-2,3 positions of xylopyranosyl (Xylp)	1018:1056	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	3	theme	xylopyranosyl	1037:1049	arg1	O-5					1063:1065	O-5	1063:1065	O-5 of arabinofuranosyl (Araf) residues	1063:1101	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	0	4	theme	antiviral	97:105	arg1	arabinoxylans					20:32	Chemically sulfated arabinoxylans	0:32	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.	0:115	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	0	4	theme	antiviral	97:105	arg1	activity					107:114	antiviral activity	97:114	antiviral activity	97:114	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	2	5	theme	action	299:304	arg1	mode					291:294	their unique mode	278:294	their unique mode of action	278:304	Polysaccharide sulfates owing to their unique mode of action are prominent antiviral drug candidates.					
33483058	6	6	theme	μg	944:945	arg1	mL-1					947:950	2.9 μg mL-1	940:950	IC50: 2.9 μg mL-1	934:950	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	3	7	theme	Plantago	375:382	arg1	husk					395:398	Plantago ovata seed husk	375:398	Plantago ovata seed husk	375:398	Herein, the arabinoxylan of Plantago ovata seed husk was simultaneously extracted and chemically sulfated using sulphur trioxide-pyridine reagent in N,N-dimethylformamide solvent (SO3⋅Py/DMF).					
33483058	0	8	from	husk	59:62	arg1	arabinoxylans					20:32	Chemically sulfated arabinoxylans	0:32	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.	0:115	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	0	8	from	husk	59:62	arg1	characterization					76:91	characterization	76:91	characterization	76:91	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	0	8	from	husk	59:62	arg1	activity					107:114	antiviral activity	97:114	antiviral activity	97:114	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	0	8	from	husk	59:62	arg1	Synthesis					65:73	Synthesis	65:73	Synthesis	65:73	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	6	9	theme	2.9	940:942	arg1	μg					944:945	μg	944:945	μg	944:945	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	10	theme	residues	1094:1101	arg1	O-3					1010:1012	O-3	1010:1012	O-3	1010:1012	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	10	theme	residues	1094:1101	arg1	positions					1024:1032	O-2,3 positions	1018:1032	O-2,3 positions of xylopyranosyl (Xylp)	1018:1056	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	10	theme	residues	1094:1101	arg1	O-5					1063:1065	O-5	1063:1065	O-5 of arabinofuranosyl (Araf) residues	1063:1101	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	4	11	theme	::	701:702	arg1	10-19:81-90					703:713	Xyl::10-19:81-90	698:713	Ara: Xyl::10-19:81-90; molar ratio	693:726	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	4	12	theme	Xyl	698:700	arg1	10-19:81-90					703:713	Xyl::10-19:81-90	698:713	Ara: Xyl::10-19:81-90; molar ratio	693:726	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	4	13	theme	molar	716:720	arg1	ratio					722:726	molar ratio	716:726	Ara: Xyl::10-19:81-90; molar ratio	693:726	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	4	14	dep	makeup	685:690	arg1	Ara					693:695	Ara	693:695	Ara: Xyl::10-19:81-90; molar ratio	693:726	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	3	15	theme	sulphur	459:465	arg1	reagent					485:491	sulphur trioxide-pyridine reagent	459:491	sulphur trioxide-pyridine reagent	459:491	Herein, the arabinoxylan of Plantago ovata seed husk was simultaneously extracted and chemically sulfated using sulphur trioxide-pyridine reagent in N,N-dimethylformamide solvent (SO3⋅Py/DMF).					
33483058	4	16	theme	sulfation	618:626	arg1	masses					649:654	molar masses	643:654	molar masses (18.4-31.3 kDa)	643:670	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	4	16	theme	sulfation	618:626	arg1	kDa					667:669	18.4-31.3 kDa	657:669	18.4-31.3 kDa	657:669	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	4	16	theme	sulfation	618:626	arg1	degrees					607:613	variable degrees	598:613	variable degrees of sulfation (DS: 0.1-0.9)	598:640	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	4	16	theme	sulfation	618:626	arg1	makeup					685:690	glycosyl makeup	676:690	glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio)	676:727	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	8	17	theme	molecules	1265:1273	arg1	addition					1322:1329	a useful addition	1313:1329	a useful addition to the arsenal of antivirals	1313:1358	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	8	17	theme	molecules	1265:1273	arg1	synthesis					1248:1256	synthesis	1248:1256	synthesis of new molecules possessing diverse structures	1248:1303	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	2	18	theme	unique	284:289	arg1	mode					291:294	their unique mode	278:294	their unique mode of action	278:304	Polysaccharide sulfates owing to their unique mode of action are prominent antiviral drug candidates.					
33483058	7	19	dep	mL-1	1180:1183	arg1	μg					1177:1178	μg	1177:1178	μg	1177:1178	Besides, this polymer showed no cytotoxicity at concentration up to 1000 μg mL-1.					
33483058	1	20	theme	viral	182:186	arg1	infections					188:197	viral infections	182:197	viral infections	182:197	Limited options for the treatments of diseases triggered through viral infections revealed the quest for novel antiviral drugs.					
33483058	0	21	theme	sulfated	11:18	arg1	arabinoxylans					20:32	Chemically sulfated arabinoxylans	0:32	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.	0:115	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	0	21	theme	sulfated	11:18	arg1	characterization					76:91	characterization	76:91	characterization	76:91	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	0	21	theme	sulfated	11:18	arg1	activity					107:114	antiviral activity	97:114	antiviral activity	97:114	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	0	21	theme	sulfated	11:18	arg1	Synthesis					65:73	Synthesis	65:73	Synthesis	65:73	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	6	22	theme	O-2,3	1018:1022	arg1	positions					1024:1032	O-2,3 positions	1018:1032	O-2,3 positions of xylopyranosyl (Xylp)	1018:1056	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	2	23	theme	drug	330:333	arg1	candidates					335:344	prominent antiviral drug candidates	310:344	prominent antiviral drug candidates	310:344	Polysaccharide sulfates owing to their unique mode of action are prominent antiviral drug candidates.					
33483058	2	23	theme	drug	330:333	arg1	sulfates					260:267	Polysaccharide sulfates	245:267	Polysaccharide sulfates owing to their unique mode of action	245:304	Polysaccharide sulfates owing to their unique mode of action are prominent antiviral drug candidates.					
33483058	6	24	contain	possessing	981:990	arg1	compound					916:923	The utmost effective compound	895:923	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1)	895:951	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	24	contain	possessing	981:990	arg1	arabinoxylan					968:979	a 18.4 kDa arabinoxylan	957:979	a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues	957:1101	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	24	contain	possessing	981:990	arg2	groups					1000:1005	sulfate groups	992:1005	sulfate groups	992:1005	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	25	theme	kDa	964:966	arg1	compound					916:923	The utmost effective compound	895:923	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1)	895:951	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	25	theme	kDa	964:966	arg1	arabinoxylan					968:979	a 18.4 kDa arabinoxylan	957:979	a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues	957:1101	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	26	dep	IC50	934:937	arg1	mL-1					947:950	2.9 μg mL-1	940:950	IC50: 2.9 μg mL-1	934:950	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	5	27	theme	virus	838:842	arg1	type					844:847	anti-herpes simplex virus type 1	818:849	anti-herpes simplex virus type 1 activity	818:858	According to the results, these polymers displayed anti-herpes simplex virus type 1 activity and their potency depends upon DS.					
33483058	2	28	theme	antiviral	320:328	arg1	candidates					335:344	prominent antiviral drug candidates	310:344	prominent antiviral drug candidates	310:344	Polysaccharide sulfates owing to their unique mode of action are prominent antiviral drug candidates.					
33483058	2	28	theme	antiviral	320:328	arg1	sulfates					260:267	Polysaccharide sulfates	245:267	Polysaccharide sulfates owing to their unique mode of action	245:304	Polysaccharide sulfates owing to their unique mode of action are prominent antiviral drug candidates.					
33483058	3	29	theme	ovata	384:388	arg1	husk					395:398	Plantago ovata seed husk	375:398	Plantago ovata seed husk	375:398	Herein, the arabinoxylan of Plantago ovata seed husk was simultaneously extracted and chemically sulfated using sulphur trioxide-pyridine reagent in N,N-dimethylformamide solvent (SO3⋅Py/DMF).					
33483058	2	30	theme	prominent	310:318	arg1	candidates					335:344	prominent antiviral drug candidates	310:344	prominent antiviral drug candidates	310:344	Polysaccharide sulfates owing to their unique mode of action are prominent antiviral drug candidates.					
33483058	2	30	theme	prominent	310:318	arg1	sulfates					260:267	Polysaccharide sulfates	245:267	Polysaccharide sulfates owing to their unique mode of action	245:304	Polysaccharide sulfates owing to their unique mode of action are prominent antiviral drug candidates.					
33483058	8	31	theme	useful	1315:1320	arg1	addition					1322:1329	a useful addition	1313:1329	a useful addition to the arsenal of antivirals	1313:1358	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	8	31	theme	useful	1315:1320	arg1	synthesis					1248:1256	synthesis	1248:1256	synthesis of new molecules possessing diverse structures	1248:1303	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	4	32	theme	molar	643:647	arg1	masses					649:654	molar masses	643:654	molar masses (18.4-31.3 kDa)	643:670	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	4	32	theme	molar	643:647	arg1	kDa					667:669	18.4-31.3 kDa	657:669	18.4-31.3 kDa	657:669	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	3	33	theme	seed	390:393	arg1	husk					395:398	Plantago ovata seed husk	375:398	Plantago ovata seed husk	375:398	Herein, the arabinoxylan of Plantago ovata seed husk was simultaneously extracted and chemically sulfated using sulphur trioxide-pyridine reagent in N,N-dimethylformamide solvent (SO3⋅Py/DMF).					
33483058	4	34	theme	variable	598:605	arg1	degrees					607:613	variable degrees	598:613	variable degrees of sulfation (DS: 0.1-0.9)	598:640	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	7	35	dep	1000	1172:1175	arg1	to					1169:1170	to	1169:1170	to	1169:1170	Besides, this polymer showed no cytotoxicity at concentration up to 1000 μg mL-1.					
33483058	5	36	theme	type	844:847	arg1	activity					851:858	anti-herpes simplex virus type 1 activity	818:858	anti-herpes simplex virus type 1 activity	818:858	According to the results, these polymers displayed anti-herpes simplex virus type 1 activity and their potency depends upon DS.					
33483058	0	37	theme	ovata	48:52	arg1	husk					59:62	Plantago ovata seed husk	39:62	Plantago ovata seed husk	39:62	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	2	38	theme	Polysaccharide	245:258	arg1	candidates					335:344	prominent antiviral drug candidates	310:344	prominent antiviral drug candidates	310:344	Polysaccharide sulfates owing to their unique mode of action are prominent antiviral drug candidates.					
33483058	2	38	theme	Polysaccharide	245:258	arg1	sulfates					260:267	Polysaccharide sulfates	245:267	Polysaccharide sulfates owing to their unique mode of action	245:304	Polysaccharide sulfates owing to their unique mode of action are prominent antiviral drug candidates.					
33483058	5	39	theme	anti-herpes	818:828	arg1	type					844:847	anti-herpes simplex virus type 1	818:849	anti-herpes simplex virus type 1 activity	818:858	According to the results, these polymers displayed anti-herpes simplex virus type 1 activity and their potency depends upon DS.					
33483058	0	40	theme	Plantago	39:46	arg1	husk					59:62	Plantago ovata seed husk	39:62	Plantago ovata seed husk	39:62	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	4	41	theme	arabinoxylan	552:563	arg1	sulfates					565:572	three arabinoxylan sulfates	546:572	three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio)	546:727	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	4	41	theme	arabinoxylan	552:563	arg1	IS1201-IS1203					575:587	IS1201-IS1203	575:587	IS1201-IS1203	575:587	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	7	42	dep	concentration	1152:1164	arg1	mL-1					1180:1183	up to 1000 μg mL-1	1166:1183	concentration up to 1000 μg mL-1	1152:1183	Besides, this polymer showed no cytotoxicity at concentration up to 1000 μg mL-1.					
33483058	1	43	theme	novel	222:226	arg1	drugs					238:242	novel antiviral drugs	222:242	novel antiviral drugs	222:242	Limited options for the treatments of diseases triggered through viral infections revealed the quest for novel antiviral drugs.					
33483058	8	44	theme	polysaccharide	1197:1210	arg1	sulfates					1212:1219	polysaccharide sulfates	1197:1219	polysaccharide sulfates	1197:1219	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	8	45	theme	new	1261:1263	arg1	molecules					1265:1273	new molecules	1261:1273	new molecules possessing diverse structures	1261:1303	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	6	46	dep	compound	916:923	arg1	IS1203					926:931	IS1203	926:931	IS1203	926:931	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	8	47	theme	diverse	1286:1292	arg1	structures					1294:1303	diverse structures	1286:1303	diverse structures	1286:1303	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	1	48	theme	Limited	117:123	arg1	options					125:131	Limited options	117:131	Limited options for the treatments of diseases triggered through viral infections	117:197	Limited options for the treatments of diseases triggered through viral infections revealed the quest for novel antiviral drugs.					
33483058	0	49	theme	seed	54:57	arg1	husk					59:62	Plantago ovata seed husk	39:62	Plantago ovata seed husk	39:62	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	6	50	theme	effective	906:914	arg1	compound					916:923	The utmost effective compound	895:923	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1)	895:951	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	50	theme	effective	906:914	arg1	arabinoxylan					968:979	a 18.4 kDa arabinoxylan	957:979	a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues	957:1101	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	8	51	contain	have	1221:1224	arg1	sulfates					1212:1219	polysaccharide sulfates	1197:1219	polysaccharide sulfates	1197:1219	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	8	51	contain	have	1221:1224	arg2	activities					1236:1245	antiviral activities	1226:1245	antiviral activities	1226:1245	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	6	52	theme	utmost	899:904	arg1	compound					916:923	The utmost effective compound	895:923	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1)	895:951	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	52	theme	utmost	899:904	arg1	arabinoxylan					968:979	a 18.4 kDa arabinoxylan	957:979	a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues	957:1101	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	6	53	dep	IS1203	926:931	arg1	IC50					934:937	IC50	934:937	IC50: 2.9 μg mL-1	934:950	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	3	54	theme	N-dimethylformamide	498:516	arg1	SO3⋅Py/DMF					527:536	SO3⋅Py/DMF	527:536	SO3⋅Py/DMF	527:536	Herein, the arabinoxylan of Plantago ovata seed husk was simultaneously extracted and chemically sulfated using sulphur trioxide-pyridine reagent in N,N-dimethylformamide solvent (SO3⋅Py/DMF).					
33483058	3	54	theme	N-dimethylformamide	498:516	arg1	N					496:496	N	496:496	N	496:496	Herein, the arabinoxylan of Plantago ovata seed husk was simultaneously extracted and chemically sulfated using sulphur trioxide-pyridine reagent in N,N-dimethylformamide solvent (SO3⋅Py/DMF).					
33483058	3	54	theme	N-dimethylformamide	498:516	arg1	solvent					518:524	N-dimethylformamide solvent	498:524	N-dimethylformamide solvent (SO3⋅Py/DMF)	498:537	Herein, the arabinoxylan of Plantago ovata seed husk was simultaneously extracted and chemically sulfated using sulphur trioxide-pyridine reagent in N,N-dimethylformamide solvent (SO3⋅Py/DMF).					
33483058	1	55	theme	antiviral	228:236	arg1	drugs					238:242	novel antiviral drugs	222:242	novel antiviral drugs	222:242	Limited options for the treatments of diseases triggered through viral infections revealed the quest for novel antiviral drugs.					
33483058	6	56	theme	Araf	1088:1091	arg1	residues					1094:1101	arabinofuranosyl (Araf) residues	1070:1101	arabinofuranosyl (Araf) residues	1070:1101	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	0	57	dep	arabinoxylans	20:32	arg1	arabinoxylans					20:32	Chemically sulfated arabinoxylans	0:32	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.	0:115	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	0	57	dep	arabinoxylans	20:32	arg1	characterization					76:91	characterization	76:91	characterization	76:91	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	0	57	dep	arabinoxylans	20:32	arg1	activity					107:114	antiviral activity	97:114	antiviral activity	97:114	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	0	57	dep	arabinoxylans	20:32	arg1	Synthesis					65:73	Synthesis	65:73	Synthesis	65:73	Chemically sulfated arabinoxylans from Plantago ovata seed husk: Synthesis, characterization and antiviral activity.					
33483058	4	58	theme	glycosyl	676:683	arg1	makeup					685:690	glycosyl makeup	676:690	glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio)	676:727	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	6	59	theme	18.4	959:962	arg1	kDa					964:966	kDa	964:966	kDa	964:966	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	5	60	theme	simplex	830:836	arg1	type					844:847	anti-herpes simplex virus type 1	818:849	anti-herpes simplex virus type 1 activity	818:858	According to the results, these polymers displayed anti-herpes simplex virus type 1 activity and their potency depends upon DS.					
33483058	8	61	theme	antivirals	1349:1358	arg1	arsenal					1338:1344	the arsenal	1334:1344	the arsenal of antivirals	1334:1358	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	7	62	dep	μg	1177:1178	arg1	1000					1172:1175	1000	1172:1175	1000	1172:1175	Besides, this polymer showed no cytotoxicity at concentration up to 1000 μg mL-1.					
33483058	3	63	theme	trioxide-pyridine	467:483	arg1	reagent					485:491	sulphur trioxide-pyridine reagent	459:491	sulphur trioxide-pyridine reagent	459:491	Herein, the arabinoxylan of Plantago ovata seed husk was simultaneously extracted and chemically sulfated using sulphur trioxide-pyridine reagent in N,N-dimethylformamide solvent (SO3⋅Py/DMF).					
33483058	6	64	theme	arabinofuranosyl	1070:1085	arg1	residues					1094:1101	arabinofuranosyl (Araf) residues	1070:1101	arabinofuranosyl (Araf) residues	1070:1101	The utmost effective compound (IS1203, IC50: 2.9 μg mL-1) was a 18.4 kDa arabinoxylan possessing sulfate groups at O-3 and O-2,3 positions of xylopyranosyl (Xylp), and O-5 of arabinofuranosyl (Araf) residues.					
33483058	3	65	theme	husk	395:398	arg1	arabinoxylan					359:370	the arabinoxylan	355:370	the arabinoxylan of Plantago ovata seed husk	355:398	Herein, the arabinoxylan of Plantago ovata seed husk was simultaneously extracted and chemically sulfated using sulphur trioxide-pyridine reagent in N,N-dimethylformamide solvent (SO3⋅Py/DMF).					
33483058	4	66	dep	Ara	693:695	arg1	ratio					722:726	molar ratio	716:726	Ara: Xyl::10-19:81-90; molar ratio	693:726	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	4	66	dep	Ara	693:695	arg1	10-19:81-90					703:713	Xyl::10-19:81-90	698:713	Ara: Xyl::10-19:81-90; molar ratio	693:726	Thus, three arabinoxylan sulfates (IS1201-IS1203) holding variable degrees of sulfation (DS: 0.1-0.9), molar masses (18.4-31.3 kDa) and glycosyl makeup (Ara: Xyl::10-19:81-90; molar ratio) were produced and then characterized.					
33483058	8	67	contain	possessing	1275:1284	arg1	molecules					1265:1273	new molecules	1261:1273	new molecules possessing diverse structures	1261:1303	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
33483058	8	67	contain	possessing	1275:1284	arg2	structures					1294:1303	diverse structures	1286:1303	diverse structures	1286:1303	Given that polysaccharide sulfates have antiviral activities, synthesis of new molecules possessing diverse structures will be a useful addition to the arsenal of antivirals.					
34652896	2	0	theme	COVID-19	451:458	arg1	treatment					438:446	the treatment	434:446	the treatment of COVID-19	434:458	PG545 has also demonstrated potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2, and shows promise as an antiviral drug for the treatment of COVID-19.					
34652896	1	1	theme	mimetic	134:140	arg1	inhibitor					179:187	a highly potent inhibitor	163:187	a highly potent inhibitor of angiogenesis, tumor growth, and metastasis	163:233	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	1	theme	mimetic	134:140	arg1	PG545					153:157	PG545	153:157	PG545	153:157	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	1	theme	mimetic	134:140	arg1	pixatimod					142:150	The heparan sulfate (HS) mimetic pixatimod	109:150	The heparan sulfate (HS) mimetic pixatimod (PG545)	109:158	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	2	theme	growth	212:217	arg1	pixatimod					142:150	The heparan sulfate (HS) mimetic pixatimod	109:150	The heparan sulfate (HS) mimetic pixatimod (PG545)	109:158	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	2	theme	growth	212:217	arg1	inhibitor					179:187	a highly potent inhibitor	163:187	a highly potent inhibitor of angiogenesis, tumor growth, and metastasis	163:233	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	6	3	theme	protecting	887:896	arg1	groups					898:903	more stable protecting groups	875:903	more stable protecting groups	875:903	Particular attention was given to improving the key glycosylation step by using more stable protecting groups and optimized glycosyl donors.					
34652896	6	4	theme	Particular	795:804	arg1	attention					806:814	Particular attention	795:814	Particular attention	795:814	Particular attention was given to improving the key glycosylation step by using more stable protecting groups and optimized glycosyl donors.					
34652896	2	5	theme	antiviral	311:319	arg1	activity					321:328	potent antiviral activity	304:328	potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2,	304:389	PG545 has also demonstrated potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2, and shows promise as an antiviral drug for the treatment of COVID-19.					
34652896	0	6	theme	Oligosaccharide-Steroid	74:96	arg1	Pixatimod					44:52	Pixatimod	44:52	Pixatimod (PG545)	44:60	Development of Improved Synthetic Routes to Pixatimod (PG545), a Sulfated Oligosaccharide-Steroid Conjugate.					
34652896	0	6	theme	Oligosaccharide-Steroid	74:96	arg1	Conjugate					98:106	a Sulfated Oligosaccharide-Steroid Conjugate	63:106	a Sulfated Oligosaccharide-Steroid Conjugate	63:106	Development of Improved Synthetic Routes to Pixatimod (PG545), a Sulfated Oligosaccharide-Steroid Conjugate.					
34652896	6	7	theme	stable	880:885	arg1	groups					898:903	more stable protecting groups	875:903	more stable protecting groups	875:903	Particular attention was given to improving the key glycosylation step by using more stable protecting groups and optimized glycosyl donors.					
34652896	3	8	theme	sulfated	501:508	arg1	tetrasaccharide					510:524	a fully sulfated tetrasaccharide	493:524	a fully sulfated tetrasaccharide conjugated to the steroid 5α-cholestan-3β-ol	493:569	Structurally, PG545 consists of a fully sulfated tetrasaccharide conjugated to the steroid 5α-cholestan-3β-ol.					
34652896	4	9	theme	reported	576:583	arg1	synthesis					585:593	The reported synthesis	572:593	The reported synthesis of PG545	572:602	The reported synthesis of PG545 suffers from a low yield and poor selectivity in the critical glycosylation step.					
34652896	0	10	theme	Sulfated	65:72	arg1	Pixatimod					44:52	Pixatimod	44:52	Pixatimod (PG545)	44:60	Development of Improved Synthetic Routes to Pixatimod (PG545), a Sulfated Oligosaccharide-Steroid Conjugate.					
34652896	0	10	theme	Sulfated	65:72	arg1	Conjugate					98:106	a Sulfated Oligosaccharide-Steroid Conjugate	63:106	a Sulfated Oligosaccharide-Steroid Conjugate	63:106	Development of Improved Synthetic Routes to Pixatimod (PG545), a Sulfated Oligosaccharide-Steroid Conjugate.					
34652896	1	11	theme	metastasis	224:233	arg1	pixatimod					142:150	The heparan sulfate (HS) mimetic pixatimod	109:150	The heparan sulfate (HS) mimetic pixatimod (PG545)	109:158	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	11	theme	metastasis	224:233	arg1	inhibitor					179:187	a highly potent inhibitor	163:187	a highly potent inhibitor of angiogenesis, tumor growth, and metastasis	163:233	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	5	12	theme	new	717:719	arg1	routes					731:736	new efficient routes	717:736	new efficient routes for the synthesis of PG545 and analogues	717:777	Given its clinical importance, new efficient routes for the synthesis of PG545 and analogues were developed.					
34652896	4	13	theme	poor	633:636	arg1	selectivity					638:648	poor selectivity	633:648	poor selectivity	633:648	The reported synthesis of PG545 suffers from a low yield and poor selectivity in the critical glycosylation step.					
34652896	5	14	theme	efficient	721:729	arg1	routes					731:736	new efficient routes	717:736	new efficient routes for the synthesis of PG545 and analogues	717:777	Given its clinical importance, new efficient routes for the synthesis of PG545 and analogues were developed.					
34652896	2	15	theme	antiviral	415:423	arg1	promise					401:407	promise	401:407	promise	401:407	PG545 has also demonstrated potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2, and shows promise as an antiviral drug for the treatment of COVID-19.					
34652896	2	15	theme	antiviral	415:423	arg1	drug					425:428	an antiviral drug	412:428	an antiviral drug for the treatment of COVID-19	412:458	PG545 has also demonstrated potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2, and shows promise as an antiviral drug for the treatment of COVID-19.					
34652896	3	16	theme	steroid	544:550	arg1	5α-cholestan-3β-ol					552:569	the steroid 5α-cholestan-3β-ol	540:569	the steroid 5α-cholestan-3β-ol	540:569	Structurally, PG545 consists of a fully sulfated tetrasaccharide conjugated to the steroid 5α-cholestan-3β-ol.					
34652896	2	17	theme	HS-dependent	347:358	arg1	viruses					360:366	numerous HS-dependent viruses	338:366	numerous HS-dependent viruses	338:366	PG545 has also demonstrated potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2, and shows promise as an antiviral drug for the treatment of COVID-19.					
34652896	2	17	theme	HS-dependent	347:358	arg1	SARS-CoV-2					379:388	SARS-CoV-2	379:388	SARS-CoV-2	379:388	PG545 has also demonstrated potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2, and shows promise as an antiviral drug for the treatment of COVID-19.					
34652896	0	18	theme	Synthetic	24:32	arg1	Routes					34:39	Improved Synthetic Routes	15:39	Improved Synthetic Routes to Pixatimod (PG545), a Sulfated Oligosaccharide-Steroid Conjugate	15:106	Development of Improved Synthetic Routes to Pixatimod (PG545), a Sulfated Oligosaccharide-Steroid Conjugate.					
34652896	0	19	theme	Improved	15:22	arg1	Routes					34:39	Improved Synthetic Routes	15:39	Improved Synthetic Routes to Pixatimod (PG545), a Sulfated Oligosaccharide-Steroid Conjugate	15:106	Development of Improved Synthetic Routes to Pixatimod (PG545), a Sulfated Oligosaccharide-Steroid Conjugate.					
34652896	1	20	theme	clinical	248:255	arg1	trials					257:262	clinical trials	248:262	clinical trials for cancer	248:273	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	6	21	theme	glycosylation	847:859	arg1	step					861:864	the key glycosylation step	839:864	the key glycosylation step	839:864	Particular attention was given to improving the key glycosylation step by using more stable protecting groups and optimized glycosyl donors.					
34652896	4	22	theme	glycosylation	666:678	arg1	step					680:683	the critical glycosylation step	653:683	the critical glycosylation step	653:683	The reported synthesis of PG545 suffers from a low yield and poor selectivity in the critical glycosylation step.					
34652896	4	23	theme	critical	657:664	arg1	step					680:683	the critical glycosylation step	653:683	the critical glycosylation step	653:683	The reported synthesis of PG545 suffers from a low yield and poor selectivity in the critical glycosylation step.					
34652896	6	24	theme	key	843:845	arg1	step					861:864	the key glycosylation step	839:864	the key glycosylation step	839:864	Particular attention was given to improving the key glycosylation step by using more stable protecting groups and optimized glycosyl donors.					
34652896	5	25	theme	clinical	696:703	arg1	importance					705:714	its clinical importance	692:714	its clinical importance	692:714	Given its clinical importance, new efficient routes for the synthesis of PG545 and analogues were developed.					
34652896	1	26	theme	potent	172:177	arg1	pixatimod					142:150	The heparan sulfate (HS) mimetic pixatimod	109:150	The heparan sulfate (HS) mimetic pixatimod (PG545)	109:158	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	26	theme	potent	172:177	arg1	inhibitor					179:187	a highly potent inhibitor	163:187	a highly potent inhibitor of angiogenesis, tumor growth, and metastasis	163:233	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	0	27	theme	Routes	34:39	arg1	Development					0:10	Development	0:10	Development of Improved Synthetic Routes to Pixatimod (PG545), a Sulfated Oligosaccharide-Steroid Conjugate.	0:107	Development of Improved Synthetic Routes to Pixatimod (PG545), a Sulfated Oligosaccharide-Steroid Conjugate.					
34652896	5	28	theme	PG545	759:763	arg1	synthesis					746:754	the synthesis	742:754	the synthesis of PG545 and analogues	742:777	Given its clinical importance, new efficient routes for the synthesis of PG545 and analogues were developed.					
34652896	4	29	theme	low	619:621	arg1	yield					623:627	a low yield	617:627	a low yield	617:627	The reported synthesis of PG545 suffers from a low yield and poor selectivity in the critical glycosylation step.					
34652896	1	30	from	inhibitor	179:187	arg1	trials					257:262	clinical trials	248:262	clinical trials for cancer	248:273	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	2	31	theme	potent	304:309	arg1	activity					321:328	potent antiviral activity	304:328	potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2,	304:389	PG545 has also demonstrated potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2, and shows promise as an antiviral drug for the treatment of COVID-19.					
34652896	5	32	theme	analogues	769:777	arg1	synthesis					746:754	the synthesis	742:754	the synthesis of PG545 and analogues	742:777	Given its clinical importance, new efficient routes for the synthesis of PG545 and analogues were developed.					
34652896	6	33	theme	glycosyl	919:926	arg1	donors					928:933	optimized glycosyl donors	909:933	optimized glycosyl donors	909:933	Particular attention was given to improving the key glycosylation step by using more stable protecting groups and optimized glycosyl donors.					
34652896	1	34	theme	heparan	113:119	arg1	sulfate					121:127	heparan sulfate	113:127	The heparan sulfate (HS) mimetic pixatimod (PG545)	109:158	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	34	theme	heparan	113:119	arg1	HS					130:131	HS	130:131	HS	130:131	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	6	35	theme	optimized	909:917	arg1	donors					928:933	optimized glycosyl donors	909:933	optimized glycosyl donors	909:933	Particular attention was given to improving the key glycosylation step by using more stable protecting groups and optimized glycosyl donors.					
34652896	1	36	theme	sulfate	121:127	arg1	inhibitor					179:187	a highly potent inhibitor	163:187	a highly potent inhibitor of angiogenesis, tumor growth, and metastasis	163:233	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	36	theme	sulfate	121:127	arg1	PG545					153:157	PG545	153:157	PG545	153:157	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	36	theme	sulfate	121:127	arg1	pixatimod					142:150	The heparan sulfate (HS) mimetic pixatimod	109:150	The heparan sulfate (HS) mimetic pixatimod (PG545)	109:158	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	37	theme	angiogenesis	192:203	arg1	pixatimod					142:150	The heparan sulfate (HS) mimetic pixatimod	109:150	The heparan sulfate (HS) mimetic pixatimod (PG545)	109:158	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	1	37	theme	angiogenesis	192:203	arg1	inhibitor					179:187	a highly potent inhibitor	163:187	a highly potent inhibitor of angiogenesis, tumor growth, and metastasis	163:233	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34652896	2	38	theme	numerous	338:345	arg1	viruses					360:366	numerous HS-dependent viruses	338:366	numerous HS-dependent viruses	338:366	PG545 has also demonstrated potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2, and shows promise as an antiviral drug for the treatment of COVID-19.					
34652896	2	38	theme	numerous	338:345	arg1	SARS-CoV-2					379:388	SARS-CoV-2	379:388	SARS-CoV-2	379:388	PG545 has also demonstrated potent antiviral activity against numerous HS-dependent viruses, including SARS-CoV-2, and shows promise as an antiviral drug for the treatment of COVID-19.					
34652896	4	39	theme	PG545	598:602	arg1	synthesis					585:593	The reported synthesis	572:593	The reported synthesis of PG545	572:602	The reported synthesis of PG545 suffers from a low yield and poor selectivity in the critical glycosylation step.					
34652896	1	40	theme	tumor	206:210	arg1	growth					212:217	tumor growth	206:217	tumor growth	206:217	The heparan sulfate (HS) mimetic pixatimod (PG545) is a highly potent inhibitor of angiogenesis, tumor growth, and metastasis currently in clinical trials for cancer.					
34285147	7	0	theme	multiple	1424:1431	arg1	testing					1433:1439	multiple testing	1424:1439	multiple testing	1424:1439	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	5	1	from	changes	863:869	arg1	traits					881:886	glycan traits	874:886	glycan traits preincident hypertension	874:911	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	1	2	theme	immune	152:157	arg1	system					159:164	the immune system	148:164	the immune system	148:164	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	6	3	from	models	1210:1215	arg1	cohort					1229:1234	the KORA cohort	1220:1234	the KORA cohort	1220:1234	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	6	4	theme	10 001	1168:1173	arg1	Dalmatians					1175:1184	10 001 Dalmatians	1168:1184	10 001 Dalmatians	1168:1184	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	3	5	theme	BP	689:690	arg1	measurement					692:702	longitudinal BP measurement available	676:712	longitudinal BP measurement available	676:712	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	1	6	theme	system	159:164	arg1	regulator					135:143	an important regulator	122:143	an important regulator of the immune system	122:164	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	1	6	theme	system	159:164	arg1	Glycosylation					79:91	OBJECTIVES Glycosylation	68:91	OBJECTIVES Glycosylation of immunoglobulin G (IgG)	68:117	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	8	7	theme	incident	1633:1640	arg1	hypertension					1642:1653	incident hypertension	1633:1653	incident hypertension	1633:1653	We then linearly combined the four replicated glycans and found that the glycan score correlated with incident hypertension, SBP and DBP.					
34285147	6	8	theme	independent	1099:1109	arg1	cohorts					1111:1117	the two independent cohorts	1091:1117	the two independent cohorts	1091:1117	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	4	9	from	Dalmatians	777:786	arg1	individuals					749:759	106 individuals	745:759	106 individuals from the 10 001 Dalmatians	745:786	We replicated our findings in 106 individuals from the 10 001 Dalmatians and 729 from KORA S4.					
34285147	1	10	theme	OBJECTIVES	68:77	arg1	regulator					135:143	an important regulator	122:143	an important regulator of the immune system	122:164	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	1	10	theme	OBJECTIVES	68:77	arg1	Glycosylation					79:91	OBJECTIVES Glycosylation	68:91	OBJECTIVES Glycosylation of immunoglobulin G (IgG)	68:117	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	5	11	dep	preincident	888:898	arg1	hypertension					900:911	hypertension	900:911	hypertension	900:911	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	7	12	dep	0.24-0.84	1483:1491	arg1	to					1503:1504	to	1503:1504	to	1503:1504	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	12	dep	0.24-0.84	1483:1491	arg1	1.5-5.68					1511:1518	1.5-5.68	1511:1518	1.5-5.68	1511:1518	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	12	dep	0.24-0.84	1483:1491	arg1	2.9					1506:1508	2.9	1506:1508	2.9	1506:1508	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	3	13	theme	mean	565:568	arg1	time					580:583	mean follow-up time	565:583	mean follow-up time of 6.3 years	565:596	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	3	14	theme	TwinsUK	549:555	arg1	cohort					557:562	the TwinsUK cohort	545:562	the TwinsUK cohort	545:562	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	2	15	theme	IgG	269:271	arg1	glycome					273:279	the IgG glycome	265:279	the IgG glycome	265:279	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	7	16	theme	%	1458:1458	arg1	ratio					1449:1453	[hazard ratio	1441:1453	[hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4	1441:1527	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	16	theme	%	1458:1458	arg1	CI					1460:1461	95% CI	1456:1461	95% CI	1456:1461	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	17	theme	hypertension	1376:1387	arg1	predictive					1353:1362	predictive	1353:1362	predictive	1353:1362	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	6	18	theme	hypertension	1046:1057	arg1	associations					1059:1070	Significant IgG-incident hypertension associations	1021:1070	Significant IgG-incident hypertension associations	1021:1070	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	6	19	from	Dalmatians	1175:1184	arg1	cohort					1229:1234	the KORA cohort	1220:1234	the KORA cohort	1220:1234	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	2	20	theme	large	376:380	arg1	cohort					406:411	a large population-based female cohort	374:411	a large population-based female cohort with two independent replication samples	374:452	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	6	21	theme	Significant	1021:1031	arg1	associations					1059:1070	Significant IgG-incident hypertension associations	1021:1070	Significant IgG-incident hypertension associations	1021:1070	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	3	22	with	controls	531:538	arg1	hypertension					509:520	incident hypertension	500:520	incident hypertension	500:520	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	1	23	theme	immunoglobulin	96:109	arg1	IgG					114:116	IgG	114:116	IgG	114:116	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	1	23	theme	immunoglobulin	96:109	arg1	G					111:111	immunoglobulin G	96:111	immunoglobulin G (IgG)	96:117	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	9	24	dep	CONCLUSION	1669:1678	arg1	suggest					1692:1698	suggest	1692:1698	suggest that the IgG glycome changes prior to the development of hypertension	1692:1768	CONCLUSION Our results suggest that the IgG glycome changes prior to the development of hypertension.					
34285147	9	25	theme	IgG	1709:1711	arg1	glycome					1713:1719	the IgG glycome	1705:1719	the IgG glycome	1705:1719	CONCLUSION Our results suggest that the IgG glycome changes prior to the development of hypertension.					
34285147	2	26	with	cohort	406:411	arg1	samples					446:452	two independent replication samples	418:452	two independent replication samples	418:452	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	3	27	with	cases	489:493	arg1	hypertension					509:520	incident hypertension	500:520	incident hypertension	500:520	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	1	28	theme	G	111:111	arg1	regulator					135:143	an important regulator	122:143	an important regulator of the immune system	122:164	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	1	28	theme	G	111:111	arg1	Glycosylation					79:91	OBJECTIVES Glycosylation	68:91	OBJECTIVES Glycosylation of immunoglobulin G (IgG)	68:117	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	2	29	theme	replication	434:444	arg1	samples					446:452	two independent replication samples	418:452	two independent replication samples	418:452	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	6	30	dep	Dalmatians	1175:1184	arg1	the					1164:1166	the	1164:1166	the	1164:1166	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	3	31	theme	follow-up	570:578	arg1	time					580:583	mean follow-up time	565:583	mean follow-up time of 6.3 years	565:596	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	9	32	theme	hypertension	1757:1768	arg1	development					1742:1752	the development	1738:1752	the development of hypertension	1738:1768	CONCLUSION Our results suggest that the IgG glycome changes prior to the development of hypertension.					
34285147	5	33	theme	glycan	874:879	arg1	traits					881:886	glycan traits	874:886	glycan traits preincident hypertension	874:911	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	0	34	theme	G	34:34	arg1	N-glycosylation					0:14	N-glycosylation	0:14	N-glycosylation of immunoglobulin G	0:34	N-glycosylation of immunoglobulin G predicts incident hypertension.					
34285147	5	35	theme	Cox	810:812	arg1	models					831:836	Cox regression mixed models	810:836	Cox regression mixed models	810:836	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	5	36	theme	arterial	944:951	arg1	pressure					953:960	mean arterial pressure	939:960	mean arterial pressure	939:960	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	7	37	theme	incident	1367:1374	arg1	hypertension					1376:1387	incident hypertension	1367:1387	incident hypertension	1367:1387	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	38	theme	GlcNAc	1317:1322	arg1	traits					1286:1291	four glycan traits	1274:1291	four glycan traits	1274:1291	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	38	theme	GlcNAc	1317:1322	arg1	GP21					1338:1341	GP21	1338:1341	GP21	1338:1341	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	38	theme	GlcNAc	1317:1322	arg1	GP9					1330:1332	GP9	1330:1332	GP9	1330:1332	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	38	theme	GlcNAc	1317:1322	arg1	GP4					1325:1327	GP4	1325:1327	GP4	1325:1327	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	38	theme	GlcNAc	1317:1322	arg1	incidence					1294:1302	incidence	1294:1302	incidence of bisecting GlcNAc	1294:1322	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	0	39	theme	immunoglobulin	19:32	arg1	G					34:34	immunoglobulin G	19:34	immunoglobulin G	19:34	N-glycosylation of immunoglobulin G predicts incident hypertension.					
34285147	5	40	theme	mean	939:942	arg1	pressure					953:960	mean arterial pressure	939:960	mean arterial pressure	939:960	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	6	41	theme	regression	1199:1208	arg1	models					1210:1215	logistic regression models	1190:1215	logistic regression models	1190:1215	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	5	42	theme	regression	814:823	arg1	models					831:836	Cox regression mixed models	810:836	Cox regression mixed models	810:836	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	1	43	theme	prevalent	193:201	arg1	hypertension					203:214	prevalent hypertension	193:214	prevalent hypertension	193:214	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	0	44	theme	incident	45:52	arg1	hypertension					54:65	incident hypertension	45:65	incident hypertension	45:65	N-glycosylation of immunoglobulin G predicts incident hypertension.					
34285147	7	45	theme	bisecting	1307:1315	arg1	GlcNAc					1317:1322	bisecting GlcNAc	1307:1322	bisecting GlcNAc	1307:1322	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	46	dep	RESULTS	1237:1243	arg1	replicated					1263:1272	replicated	1263:1272	replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4]	1263:1528	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	46	dep	RESULTS	1237:1243	arg1	identified					1248:1257	identified	1248:1257	identified	1248:1257	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	3	47	theme	ultra-performance	632:648	arg1	chromatography					657:670	ultra-performance liquid chromatography	632:670	ultra-performance liquid chromatography	632:670	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	0	48	gly	N-glycosylation	0:14	arg1	G					34:34	immunoglobulin G	19:34	immunoglobulin G	19:34	N-glycosylation of immunoglobulin G predicts incident hypertension.					
34285147	2	49	theme	female	399:404	arg1	cohort					406:411	a large population-based female cohort	374:411	a large population-based female cohort with two independent replication samples	374:452	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	2	50	theme	independent	422:432	arg1	samples					446:452	two independent replication samples	418:452	two independent replication samples	418:452	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	2	51	theme	population-based	382:397	arg1	cohort					406:411	a large population-based female cohort	374:411	a large population-based female cohort with two independent replication samples	374:452	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	3	52	theme	incident	500:507	arg1	hypertension					509:520	incident hypertension	500:520	incident hypertension	500:520	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	5	53	theme	mixed	825:829	arg1	models					831:836	Cox regression mixed models	810:836	Cox regression mixed models	810:836	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	6	54	theme	Cox	1133:1135	arg1	models					1154:1159	Cox regression mixed models	1133:1159	Cox regression mixed models	1133:1159	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	6	55	theme	mixed	1148:1152	arg1	models					1154:1159	Cox regression mixed models	1133:1159	Cox regression mixed models	1133:1159	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	6	56	theme	logistic	1190:1197	arg1	models					1210:1215	logistic regression models	1190:1215	logistic regression models	1190:1215	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	6	57	theme	regression	1137:1146	arg1	models					1154:1159	Cox regression mixed models	1133:1159	Cox regression mixed models	1133:1159	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	3	58	dep	included	466:473	arg1	time					580:583	mean follow-up time	565:583	mean follow-up time of 6.3 years	565:596	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	3	59	theme	longitudinal	676:687	arg1	measurement					692:702	longitudinal BP measurement available	676:712	longitudinal BP measurement available	676:712	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	6	60	theme	KORA	1224:1227	arg1	cohort					1229:1234	the KORA cohort	1220:1234	the KORA cohort	1220:1234	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
34285147	3	61	theme	years	592:596	arg1	time					580:583	mean follow-up time	565:583	mean follow-up time of 6.3 years	565:596	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	1	62	theme	important	125:133	arg1	regulator					135:143	an important regulator	122:143	an important regulator of the immune system	122:164	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	1	62	theme	important	125:133	arg1	Glycosylation					79:91	OBJECTIVES Glycosylation	68:91	OBJECTIVES Glycosylation of immunoglobulin G (IgG)	68:117	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	5	63	theme	family	968:973	arg1	relatedness					975:985	family relatedness	968:985	family relatedness	968:985	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	3	64	theme	liquid	650:655	arg1	chromatography					657:670	ultra-performance liquid chromatography	632:670	ultra-performance liquid chromatography	632:670	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	3	65	dep	METHODS	455:461	arg1	included					466:473	included	466:473	included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available	466:712	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	3	66	theme	unrelated	479:487	arg1	cases					489:493	989 unrelated cases	475:493	989 unrelated cases with incident hypertension	475:520	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	8	67	theme	glycan	1604:1609	arg1	score					1611:1615	the glycan score	1600:1615	the glycan score	1600:1615	We then linearly combined the four replicated glycans and found that the glycan score correlated with incident hypertension, SBP and DBP.					
34285147	2	68	theme	hypertension	307:318	arg1	diagnosis					320:328	hypertension diagnosis	307:328	hypertension diagnosis	307:328	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	1	69	gly	Glycosylation	79:91	arg1	IgG					114:116	IgG	114:116	IgG	114:116	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	1	69	gly	Glycosylation	79:91	arg1	hypertension					203:214	prevalent hypertension	193:214	prevalent hypertension	193:214	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	1	69	gly	Glycosylation	79:91	arg1	G					111:111	immunoglobulin G	96:111	immunoglobulin G (IgG)	96:117	OBJECTIVES Glycosylation of immunoglobulin G (IgG) is an important regulator of the immune system and has been implicated in prevalent hypertension.					
34285147	5	70	theme	preincident	888:898	arg1	traits					881:886	glycan traits	874:886	glycan traits preincident hypertension	874:911	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	5	71	theme	multiple	991:998	arg1	FDR < 0.1					1009:1017	FDR < 0.1	1009:1017	FDR < 0.1	1009:1017	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	5	71	theme	multiple	991:998	arg1	testing					1000:1006	multiple testing	991:1006	multiple testing (FDR < 0.1)	991:1018	Cox regression mixed models were applied to identify changes in glycan traits preincident hypertension, after adjusting for age, mean arterial pressure, BMI, family relatedness and multiple testing (FDR < 0.1).					
34285147	2	72	theme	glycome	351:357	arg1	composition					359:369	the IgG glycome composition	343:369	the IgG glycome composition	343:369	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	3	73	theme	available	704:712	arg1	measurement					692:702	longitudinal BP measurement available	676:712	longitudinal BP measurement available	676:712	METHODS We included 989 unrelated cases with incident hypertension and 1628 controls from the TwinsUK cohort (mean follow-up time of 6.3 years) with IgG measured at baseline by ultra-performance liquid chromatography and longitudinal BP measurement available.					
34285147	2	74	theme	study	233:237	arg1	aim					221:223	The aim	217:223	The aim of this study	217:237	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	7	75	theme	glycan	1279:1284	arg1	traits					1286:1291	four glycan traits	1274:1291	four glycan traits	1274:1291	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	75	theme	glycan	1279:1284	arg1	GP21					1338:1341	GP21	1338:1341	GP21	1338:1341	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	75	theme	glycan	1279:1284	arg1	GP9					1330:1332	GP9	1330:1332	GP9	1330:1332	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	75	theme	glycan	1279:1284	arg1	GP4					1325:1327	GP4	1325:1327	GP4	1325:1327	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	7	75	theme	glycan	1279:1284	arg1	incidence					1294:1302	incidence	1294:1302	incidence of bisecting GlcNAc	1294:1322	RESULTS We identified and replicated four glycan traits, incidence of bisecting GlcNAc, GP4, GP9 and GP21, that are predictive of incident hypertension after adjusting for confoundes and multiple testing [hazard ratio (95% CI) ranging from 0.45 (0.24-0.84) for GP21 to 2.9 (1.5-5.68) for GP4].					
34285147	2	76	theme	IgG	347:349	arg1	composition					359:369	the IgG glycome composition	343:369	the IgG glycome composition	343:369	The aim of this study is to investigate whether the IgG glycome begins to change prior to hypertension diagnosis by analysing the IgG glycome composition in a large population-based female cohort with two independent replication samples.					
34285147	8	77	theme	replicated	1566:1575	arg1	glycans					1577:1583	the four replicated glycans	1557:1583	the four replicated glycans	1557:1583	We then linearly combined the four replicated glycans and found that the glycan score correlated with incident hypertension, SBP and DBP.					
34285147	6	78	theme	IgG-incident	1033:1044	arg1	associations					1059:1070	Significant IgG-incident hypertension associations	1021:1070	Significant IgG-incident hypertension associations	1021:1070	Significant IgG-incident hypertension associations were replicated in the two independent cohorts by leveraging Cox regression mixed models in the 10 001 Dalmatians and logistic regression models in the KORA cohort.					
33794241	3	0	theme	fibroin	516:522	arg1	ASA					543:545	ASA	543:545	ASA	543:545	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	3	0	theme	fibroin	516:522	arg1	aerogel					534:540	attapulgite-based silk fibroin composite aerogel	493:540	attapulgite-based silk fibroin composite aerogel (ASA)	493:546	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	5	1	theme	%	738:738	arg1	dose					725:728	dose	725:728	follows: dose of ASA 1% and pH 7.0	716:749	Secondly, the deproteinization parameters were optimized with selectivity coefficient as index as follows: dose of ASA 1% and pH 7.0.					
33794241	6	2	theme	18.95	916:920	arg1	±					922:922	18.95 ± 1.55	916:927	18.95 ± 1.55	916:927	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	5	3	dep	follows	716:722	arg1	dose					725:728	dose	725:728	follows: dose of ASA 1% and pH 7.0	716:749	Secondly, the deproteinization parameters were optimized with selectivity coefficient as index as follows: dose of ASA 1% and pH 7.0.					
33794241	11	4	theme	polysaccharides	1510:1524	arg1	processing					1526:1535	polysaccharides processing	1510:1535	polysaccharides processing	1510:1535	Taking together, the data implied that ASA method would be promising of deproteinization from PRP suitable for polysaccharides processing.					
33794241	6	5	theme	Dr	800:801	arg1	ratio					793:797	deproteinization ratio	776:797	deproteinization ratio (Dr%)	776:803	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	6	5	theme	Dr	800:801	arg1	%					802:802	Dr%	800:802	Dr%	800:802	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	2	6	theme	organic	369:375	arg1	reagents					377:384	organic reagents	369:384	organic reagents	369:384	Moreover, the traditional Sevag deproteinization method always involves organic reagents.					
33794241	8	7	theme	deproteinization	1044:1059	arg1	result					1000:1005	a result	998:1005	a result	998:1005	As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides.					
33794241	8	7	theme	deproteinization	1044:1059	arg1	efficiency					1061:1070	higher deproteinization efficiency	1037:1070	higher deproteinization efficiency	1037:1070	As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides.					
33794241	10	8	theme	Freundlich	1381:1390	arg1	model					1392:1396	pseudo second-order kinetic and Freundlich model	1349:1396	pseudo second-order kinetic and Freundlich model	1349:1396	Furthermore, it was found that protein adsorption on ASA was better fitted by pseudo second-order kinetic and Freundlich model.					
33794241	0	9	theme	philippinarum	86:98	arg1	polysaccharides					57:71	polysaccharides	57:71	polysaccharides of Ruditapes philippinarum	57:98	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.					
33794241	6	10	theme	selectivity	846:856	arg1	Kc					871:872	Kc	871:872	Kc	871:872	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	6	10	theme	selectivity	846:856	arg1	coefficient					858:868	selectivity coefficient	846:868	selectivity coefficient (Kc)	846:873	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	0	11	theme	silk	121:124	arg1	aerogel					144:150	attapulgite-based silk fibroin composite aerogel	103:150	attapulgite-based silk fibroin composite aerogel	103:150	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.					
33794241	10	12	theme	pseudo	1349:1354	arg1	model					1392:1396	pseudo second-order kinetic and Freundlich model	1349:1396	pseudo second-order kinetic and Freundlich model	1349:1396	Furthermore, it was found that protein adsorption on ASA was better fitted by pseudo second-order kinetic and Freundlich model.					
33794241	6	13	theme	deproteinization	776:791	arg1	ratio					793:797	deproteinization ratio	776:797	deproteinization ratio (Dr%)	776:803	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	6	13	theme	deproteinization	776:791	arg1	%					802:802	Dr%	800:802	Dr%	800:802	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	1	14	theme	protein	171:177	arg1	impurity					179:186	protein impurity	171:186	protein impurity	171:186	A large amount of protein impurity severely restricts the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine.					
33794241	0	15	theme	attapulgite-based	103:119	arg1	aerogel					144:150	attapulgite-based silk fibroin composite aerogel	103:150	attapulgite-based silk fibroin composite aerogel	103:150	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.					
33794241	9	16	theme	recycling	1211:1219	arg1	ability					1221:1227	recycling ability	1211:1227	recycling ability	1211:1227	ASA was also proven with recycling ability and could be reused more than five times.					
33794241	10	17	from	adsorption	1310:1319	arg1	ASA					1324:1326	ASA	1324:1326	ASA	1324:1326	Furthermore, it was found that protein adsorption on ASA was better fitted by pseudo second-order kinetic and Freundlich model.					
33794241	3	18	from	PRP	486:488	arg1	method					474:479	an effective, green and mild deproteinization method	428:479	an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA)	428:546	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	8	19	theme	less	1075:1078	arg1	time					1080:1083	less time	1075:1083	less time	1075:1083	As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides.					
33794241	1	20	theme	impurity	179:186	arg1	amount					161:166	A large amount	153:166	A large amount of protein impurity	153:186	A large amount of protein impurity severely restricts the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine.					
33794241	1	20	theme	impurity	179:186	arg1	impurity					179:186	protein impurity	171:186	protein impurity	171:186	A large amount of protein impurity severely restricts the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine.					
33794241	0	21	theme	effective	3:11	arg1	method					46:51	An effective, green and mild deproteinization method	0:51	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.	0:151	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.					
33794241	10	22	theme	protein	1302:1308	arg1	adsorption					1310:1319	protein adsorption	1302:1319	protein adsorption on ASA	1302:1326	Furthermore, it was found that protein adsorption on ASA was better fitted by pseudo second-order kinetic and Freundlich model.					
33794241	2	23	theme	Sevag	323:327	arg1	method					346:351	the traditional Sevag deproteinization method	307:351	the traditional Sevag deproteinization method	307:351	Moreover, the traditional Sevag deproteinization method always involves organic reagents.					
33794241	3	24	theme	effective	431:439	arg1	method					474:479	an effective, green and mild deproteinization method	428:479	an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA)	428:546	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	3	25	theme	paper	407:411	arg1	purpose					391:397	The purpose	387:397	The purpose of this paper	387:411	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	6	26	theme	±	904:904	arg1	%					910:910	95.81 ± 2.95%	898:910	95.81 ± 2.95%	898:910	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	5	27	theme	deproteinization	632:647	arg1	index					707:711	index	707:711	index as follows: dose of ASA 1% and pH 7.0	707:749	Secondly, the deproteinization parameters were optimized with selectivity coefficient as index as follows: dose of ASA 1% and pH 7.0.					
33794241	5	27	theme	deproteinization	632:647	arg1	parameters					649:658	the deproteinization parameters	628:658	the deproteinization parameters	628:658	Secondly, the deproteinization parameters were optimized with selectivity coefficient as index as follows: dose of ASA 1% and pH 7.0.					
33794241	2	28	theme	traditional	311:321	arg1	method					346:351	the traditional Sevag deproteinization method	307:351	the traditional Sevag deproteinization method	307:351	Moreover, the traditional Sevag deproteinization method always involves organic reagents.					
33794241	0	29	theme	green	14:18	arg1	method					46:51	An effective, green and mild deproteinization method	0:51	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.	0:151	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.					
33794241	6	30	theme	95.81	898:902	arg1	%					910:910	95.81 ± 2.95%	898:910	95.81 ± 2.95%	898:910	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	0	31	theme	composite	134:142	arg1	aerogel					144:150	attapulgite-based silk fibroin composite aerogel	103:150	attapulgite-based silk fibroin composite aerogel	103:150	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.					
33794241	11	32	theme	ASA	1438:1440	arg1	method					1442:1447	ASA method	1438:1447	ASA method	1438:1447	Taking together, the data implied that ASA method would be promising of deproteinization from PRP suitable for polysaccharides processing.					
33794241	0	33	theme	fibroin	126:132	arg1	aerogel					144:150	attapulgite-based silk fibroin composite aerogel	103:150	attapulgite-based silk fibroin composite aerogel	103:150	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.					
33794241	3	34	theme	composite	524:532	arg1	ASA					543:545	ASA	543:545	ASA	543:545	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	3	34	theme	composite	524:532	arg1	aerogel					534:540	attapulgite-based silk fibroin composite aerogel	493:540	attapulgite-based silk fibroin composite aerogel (ASA)	493:546	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	8	35	theme	antioxidant	1145:1155	arg1	activity					1157:1164	antioxidant activity	1145:1164	antioxidant activity	1145:1164	As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides.					
33794241	0	36	theme	mild	24:27	arg1	method					46:51	An effective, green and mild deproteinization method	0:51	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.	0:151	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.					
33794241	10	37	theme	kinetic	1369:1375	arg1	model					1392:1396	pseudo second-order kinetic and Freundlich model	1349:1396	pseudo second-order kinetic and Freundlich model	1349:1396	Furthermore, it was found that protein adsorption on ASA was better fitted by pseudo second-order kinetic and Freundlich model.					
33794241	8	38	theme	polysaccharides	1169:1183	arg1	structure					1131:1139	structure	1131:1139	structure	1131:1139	As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides.					
33794241	8	38	theme	polysaccharides	1169:1183	arg1	activity					1157:1164	antioxidant activity	1145:1164	antioxidant activity	1145:1164	As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides.					
33794241	3	39	theme	mild	452:455	arg1	method					474:479	an effective, green and mild deproteinization method	428:479	an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA)	428:546	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	3	40	theme	green	442:446	arg1	method					474:479	an effective, green and mild deproteinization method	428:479	an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA)	428:546	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	8	41	theme	Sevag	1099:1103	arg1	method					1105:1110	Sevag method	1099:1110	Sevag method	1099:1110	As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides.					
33794241	10	42	theme	second-order	1356:1367	arg1	model					1392:1396	pseudo second-order kinetic and Freundlich model	1349:1396	pseudo second-order kinetic and Freundlich model	1349:1396	Furthermore, it was found that protein adsorption on ASA was better fitted by pseudo second-order kinetic and Freundlich model.					
33794241	1	43	theme	polysaccharides	226:240	arg1	application					211:221	the application	207:221	the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine	207:294	A large amount of protein impurity severely restricts the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine.					
33794241	6	44	theme	±	889:889	arg1	%					895:895	79.44 ± 1.87%	883:895	79.44 ± 1.87%	883:895	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	5	45	theme	selectivity	680:690	arg1	coefficient					692:702	selectivity coefficient	680:702	selectivity coefficient	680:702	Secondly, the deproteinization parameters were optimized with selectivity coefficient as index as follows: dose of ASA 1% and pH 7.0.					
33794241	6	46	theme	recovery	821:828	arg1	ratio					830:834	polysaccharide recovery ratio	806:834	polysaccharide recovery ratio (Rr%)	806:840	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	6	46	theme	recovery	821:828	arg1	%					839:839	Rr%	837:839	Rr%	837:839	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	11	47	theme	deproteinization	1471:1486	arg1	promising					1458:1466	promising	1458:1466	promising	1458:1466	Taking together, the data implied that ASA method would be promising of deproteinization from PRP suitable for polysaccharides processing.					
33794241	3	48	theme	attapulgite-based	493:509	arg1	ASA					543:545	ASA	543:545	ASA	543:545	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	3	48	theme	attapulgite-based	493:509	arg1	aerogel					534:540	attapulgite-based silk fibroin composite aerogel	493:540	attapulgite-based silk fibroin composite aerogel (ASA)	493:546	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	0	49	theme	deproteinization	29:44	arg1	method					46:51	An effective, green and mild deproteinization method	0:51	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.	0:151	An effective, green and mild deproteinization method for polysaccharides of Ruditapes philippinarum by attapulgite-based silk fibroin composite aerogel.					
33794241	6	50	theme	79.44	883:887	arg1	%					895:895	79.44 ± 1.87%	883:895	79.44 ± 1.87%	883:895	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	7	51	theme	ASA	969:971	arg1	method					973:978	ASA method	969:978	ASA method	969:978	Next, the feasibility of ASA method was evaluated.					
33794241	6	52	theme	polysaccharide	806:819	arg1	ratio					830:834	polysaccharide recovery ratio	806:834	polysaccharide recovery ratio (Rr%)	806:840	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	6	52	theme	polysaccharide	806:819	arg1	%					839:839	Rr%	837:839	Rr%	837:839	Under these conditions, deproteinization ratio (Dr%), polysaccharide recovery ratio (Rr%) and selectivity coefficient (Kc) reached 79.44 ± 1.87%, 95.81 ± 2.95% and 18.95 ± 1.55, respectively.					
33794241	3	53	theme	silk	511:514	arg1	ASA					543:545	ASA	543:545	ASA	543:545	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	3	53	theme	silk	511:514	arg1	aerogel					534:540	attapulgite-based silk fibroin composite aerogel	493:540	attapulgite-based silk fibroin composite aerogel (ASA)	493:546	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	8	54	theme	ASA	1008:1010	arg1	method					1012:1017	ASA method	1008:1017	ASA method	1008:1017	As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides.					
33794241	8	55	theme	higher	1037:1042	arg1	result					1000:1005	a result	998:1005	a result	998:1005	As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides.					
33794241	8	55	theme	higher	1037:1042	arg1	efficiency					1061:1070	higher deproteinization efficiency	1037:1070	higher deproteinization efficiency	1037:1070	As a result, ASA method not only achieved higher deproteinization efficiency in less time compared with Sevag method, but also retained structure and antioxidant activity of polysaccharides.					
33794241	1	56	from	application	211:221	arg1	medicine					287:294	medicine	287:294	medicine	287:294	A large amount of protein impurity severely restricts the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine.					
33794241	1	56	from	application	211:221	arg1	food					278:281	food	278:281	food	278:281	A large amount of protein impurity severely restricts the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine.					
33794241	7	57	theme	method	973:978	arg1	feasibility					954:964	the feasibility	950:964	the feasibility of ASA method	950:978	Next, the feasibility of ASA method was evaluated.					
33794241	11	58	theme	suitable	1497:1504	arg1	PRP					1493:1495	PRP	1493:1495	PRP suitable for polysaccharides processing	1493:1535	Taking together, the data implied that ASA method would be promising of deproteinization from PRP suitable for polysaccharides processing.					
33794241	2	59	theme	deproteinization	329:344	arg1	method					346:351	the traditional Sevag deproteinization method	307:351	the traditional Sevag deproteinization method	307:351	Moreover, the traditional Sevag deproteinization method always involves organic reagents.					
33794241	3	60	theme	deproteinization	457:472	arg1	method					474:479	an effective, green and mild deproteinization method	428:479	an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA)	428:546	The purpose of this paper was to develop an effective, green and mild deproteinization method from PRP by attapulgite-based silk fibroin composite aerogel (ASA).					
33794241	11	61	from	PRP	1493:1495	arg1	deproteinization					1471:1486	deproteinization	1471:1486	deproteinization from PRP suitable for polysaccharides processing	1471:1535	Taking together, the data implied that ASA method would be promising of deproteinization from PRP suitable for polysaccharides processing.					
33794241	1	62	theme	philippinarum	255:267	arg1	polysaccharides					226:240	polysaccharides	226:240	polysaccharides of Ruditapes philippinarum (PRP)	226:273	A large amount of protein impurity severely restricts the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine.					
33794241	5	63	theme	pH	744:745	arg1	dose					725:728	dose	725:728	follows: dose of ASA 1% and pH 7.0	716:749	Secondly, the deproteinization parameters were optimized with selectivity coefficient as index as follows: dose of ASA 1% and pH 7.0.					
33794241	1	64	theme	large	155:159	arg1	amount					161:166	A large amount	153:166	A large amount of protein impurity	153:186	A large amount of protein impurity severely restricts the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine.					
33794241	1	64	theme	large	155:159	arg1	impurity					179:186	protein impurity	171:186	protein impurity	171:186	A large amount of protein impurity severely restricts the application of polysaccharides of Ruditapes philippinarum (PRP) in food and medicine.					
33794241	5	65	theme	ASA	733:735	arg1	%					738:738	ASA 1%	733:738	ASA 1%	733:738	Secondly, the deproteinization parameters were optimized with selectivity coefficient as index as follows: dose of ASA 1% and pH 7.0.					
36697781	1	0	theme	post-translational	165:182	arg1	modifications					184:196	the most important protein post-translational modifications	138:196	the most important protein post-translational modifications	138:196	N-linked glycosylation is one of the most important protein post-translational modifications.					
36697781	0	1	theme	mass	86:89	arg1	spectrometry					91:102	logically derived sequence tandem mass spectrometry	52:102	logically derived sequence tandem mass spectrometry	52:102	Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry.					
36697781	7	2	theme	larger	1153:1158	arg1	N-glycans					1160:1168	larger N-glycans	1153:1168	larger N-glycans	1153:1168	Moreover, it can be applied to all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis.					
36697781	1	3	theme	modifications	184:196	arg1	one					131:133	one	131:133	one	131:133	N-linked glycosylation is one of the most important protein post-translational modifications.					
36697781	1	3	theme	modifications	184:196	arg1	modifications					184:196	the most important protein post-translational modifications	138:196	the most important protein post-translational modifications	138:196	N-linked glycosylation is one of the most important protein post-translational modifications.					
36697781	6	4	theme	spectrum	960:967	arg1	databases					969:977	the mass spectrum databases	951:977	the mass spectrum databases of oligosaccharides and N-glycan standards	951:1020	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	7	5	theme	N-glycans	1129:1137	arg1	types					1058:1062	all types	1054:1062	all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis	1054:1201	Moreover, it can be applied to all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis.					
36697781	4	6	attach	derived	604:610	arg1	mechanisms					643:652	carbohydrate dissociation mechanisms	617:652	carbohydrate dissociation mechanisms	617:652	In LODES/MSn, the sequences of successive collision-induced dissociation are derived from carbohydrate dissociation mechanisms and apply to N-glycans in an ion trap for structural determination.					
36697781	4	6	attach	derived	604:610	arg2	sequences					545:553	the sequences	541:553	the sequences of successive collision-induced dissociation	541:598	In LODES/MSn, the sequences of successive collision-induced dissociation are derived from carbohydrate dissociation mechanisms and apply to N-glycans in an ion trap for structural determination.					
36697781	6	7	theme	mass	955:958	arg1	databases					969:977	the mass spectrum databases	951:977	the mass spectrum databases of oligosaccharides and N-glycan standards	951:1020	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	3	8	theme	mass	384:387	arg1	spectrometry					389:400	logically derived sequence tandem mass spectrometry	350:400	logically derived sequence tandem mass spectrometry (LODES/MSn)	350:412	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	8	theme	mass	384:387	arg1	method					342:347	a mass spectrometry method	322:347	a mass spectrometry method	322:347	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	8	theme	mass	384:387	arg1	LODES/MSn					403:411	LODES/MSn	403:411	LODES/MSn	403:411	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	7	9	theme	acid	1187:1190	arg1	hydrolysis					1192:1201	acid hydrolysis	1187:1201	acid hydrolysis	1187:1201	Moreover, it can be applied to all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis.					
36697781	0	10	link	derived	62:68	arg1	spectrometry					91:102	logically derived sequence tandem mass spectrometry	52:102	logically derived sequence tandem mass spectrometry	52:102	Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry.					
36697781	7	11	dep	N-glycans	1067:1075	arg1	hybrid					1092:1097	hybrid	1092:1097	hybrid	1092:1097	Moreover, it can be applied to all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis.					
36697781	7	11	dep	N-glycans	1067:1075	arg1	complex					1104:1110	complex	1104:1110	complex	1104:1110	Moreover, it can be applied to all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis.					
36697781	7	11	dep	N-glycans	1067:1075	arg1	high-mannose					1078:1089	high-mannose	1078:1089	high-mannose	1078:1089	Moreover, it can be applied to all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis.					
36697781	5	12	dep	validate	725:732	arg1	applied					789:795	applied	789:795	applied this method to N-glycans extracted from soybean, ovalbumin, and IgY	789:863	We validate LODES/MSn using synthesized N-glycans and subsequently applied this method to N-glycans extracted from soybean, ovalbumin, and IgY.					
36697781	5	12	dep	validate	725:732	arg1	using					744:748	using	744:748	using synthesized N-glycans	744:770	We validate LODES/MSn using synthesized N-glycans and subsequently applied this method to N-glycans extracted from soybean, ovalbumin, and IgY.					
36697781	1	13	link	N-linked	105:112	arg1	glycosylation					114:126	N-linked glycosylation	105:126	N-linked glycosylation	105:126	N-linked glycosylation is one of the most important protein post-translational modifications.					
36697781	0	14	theme	tandem	79:84	arg1	spectrometry					91:102	logically derived sequence tandem mass spectrometry	52:102	logically derived sequence tandem mass spectrometry	52:102	Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry.					
36697781	7	15	theme	hydrolysis	1192:1201	arg1	types					1058:1062	all types	1054:1062	all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis	1054:1201	Moreover, it can be applied to all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis.					
36697781	3	16	theme	isomers	455:461	arg1	structures					432:441	the structures	428:441	the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry	428:524	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	0	17	theme	Structural	0:9	arg1	identification					11:24	Structural identification	0:24	Structural identification of N-glycan isomers	0:44	Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry.					
36697781	3	18	link	derived	360:366	arg1	spectrometry					389:400	logically derived sequence tandem mass spectrometry	350:400	logically derived sequence tandem mass spectrometry (LODES/MSn)	350:412	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	18	link	derived	360:366	arg1	method					342:347	a mass spectrometry method	322:347	a mass spectrometry method	322:347	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	18	link	derived	360:366	arg1	LODES/MSn					403:411	LODES/MSn	403:411	LODES/MSn	403:411	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	0	19	theme	N-glycan	29:36	arg1	isomers					38:44	N-glycan isomers	29:44	N-glycan isomers	29:44	Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry.					
36697781	3	20	theme	conventional	495:506	arg1	spectrometry					513:524	conventional mass spectrometry	495:524	conventional mass spectrometry	495:524	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	1	21	theme	N-linked	105:112	arg1	glycosylation					114:126	N-linked glycosylation	105:126	N-linked glycosylation	105:126	N-linked glycosylation is one of the most important protein post-translational modifications.					
36697781	4	22	theme	dissociation	630:641	arg1	mechanisms					643:652	carbohydrate dissociation mechanisms	617:652	carbohydrate dissociation mechanisms	617:652	In LODES/MSn, the sequences of successive collision-induced dissociation are derived from carbohydrate dissociation mechanisms and apply to N-glycans in an ion trap for structural determination.					
36697781	6	23	theme	N-glycans	937:945	arg1	reduction					910:918	reduction	910:918	reduction	910:918	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	6	23	theme	N-glycans	937:945	arg1	labeling					925:932	labeling	925:932	labeling of N-glycans	925:945	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	6	23	theme	N-glycans	937:945	arg1	permethylation					894:907	permethylation	894:907	permethylation	894:907	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	6	23	theme	N-glycans	937:945	arg1	databases					969:977	the mass spectrum databases	951:977	the mass spectrum databases of oligosaccharides and N-glycan standards	951:1020	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	3	24	theme	mass	508:511	arg1	spectrometry					513:524	conventional mass spectrometry	495:524	conventional mass spectrometry	495:524	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	4	25	theme	carbohydrate	617:628	arg1	mechanisms					643:652	carbohydrate dissociation mechanisms	617:652	carbohydrate dissociation mechanisms	617:652	In LODES/MSn, the sequences of successive collision-induced dissociation are derived from carbohydrate dissociation mechanisms and apply to N-glycans in an ion trap for structural determination.					
36697781	2	26	theme	N-glycan	268:275	arg1	isomers					277:283	N-glycan isomers	268:283	N-glycan isomers	268:283	Despite the importance of N-glycans, the structural determination of N-glycan isomers remains challenging.					
36697781	0	27	theme	isomers	38:44	arg1	identification					11:24	Structural identification	0:24	Structural identification of N-glycan isomers	0:44	Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry.					
36697781	4	28	theme	collision-induced	569:585	arg1	dissociation					587:598	successive collision-induced dissociation	558:598	successive collision-induced dissociation	558:598	In LODES/MSn, the sequences of successive collision-induced dissociation are derived from carbohydrate dissociation mechanisms and apply to N-glycans in an ion trap for structural determination.					
36697781	3	29	theme	N-glycan	446:453	arg1	isomers					455:461	N-glycan isomers	446:461	N-glycan isomers	446:461	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	7	30	theme	N-glycans	1067:1075	arg1	types					1058:1062	all types	1054:1062	all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis	1054:1201	Moreover, it can be applied to all types of N-glycans (high-mannose, hybrid, and complex), as well as the N-glycans degraded from larger N-glycans by any enzyme or acid hydrolysis.					
36697781	4	31	theme	structural	696:705	arg1	determination					707:719	structural determination	696:719	structural determination	696:719	In LODES/MSn, the sequences of successive collision-induced dissociation are derived from carbohydrate dissociation mechanisms and apply to N-glycans in an ion trap for structural determination.					
36697781	0	32	theme	derived	62:68	arg1	spectrometry					91:102	logically derived sequence tandem mass spectrometry	52:102	logically derived sequence tandem mass spectrometry	52:102	Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry.					
36697781	4	33	theme	successive	558:567	arg1	dissociation					587:598	successive collision-induced dissociation	558:598	successive collision-induced dissociation	558:598	In LODES/MSn, the sequences of successive collision-induced dissociation are derived from carbohydrate dissociation mechanisms and apply to N-glycans in an ion trap for structural determination.					
36697781	3	34	theme	tandem	377:382	arg1	spectrometry					389:400	logically derived sequence tandem mass spectrometry	350:400	logically derived sequence tandem mass spectrometry (LODES/MSn)	350:412	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	34	theme	tandem	377:382	arg1	method					342:347	a mass spectrometry method	322:347	a mass spectrometry method	322:347	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	34	theme	tandem	377:382	arg1	LODES/MSn					403:411	LODES/MSn	403:411	LODES/MSn	403:411	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	6	35	theme	N-glycan	1003:1010	arg1	standards					1012:1020	N-glycan standards	1003:1020	N-glycan standards	1003:1020	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	5	36	theme	synthesized	750:760	arg1	N-glycans					762:770	synthesized N-glycans	750:770	synthesized N-glycans	750:770	We validate LODES/MSn using synthesized N-glycans and subsequently applied this method to N-glycans extracted from soybean, ovalbumin, and IgY.					
36697781	2	37	theme	structural	240:249	arg1	determination					251:263	the structural determination	236:263	the structural determination of N-glycan isomers	236:283	Despite the importance of N-glycans, the structural determination of N-glycan isomers remains challenging.					
36697781	4	38	theme	dissociation	587:598	arg1	sequences					545:553	the sequences	541:553	the sequences of successive collision-induced dissociation	541:598	In LODES/MSn, the sequences of successive collision-induced dissociation are derived from carbohydrate dissociation mechanisms and apply to N-glycans in an ion trap for structural determination.					
36697781	3	39	theme	mass	324:327	arg1	spectrometry					389:400	logically derived sequence tandem mass spectrometry	350:400	logically derived sequence tandem mass spectrometry (LODES/MSn)	350:412	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	39	theme	mass	324:327	arg1	method					342:347	a mass spectrometry method	322:347	a mass spectrometry method	322:347	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	40	theme	spectrometry	329:340	arg1	spectrometry					389:400	logically derived sequence tandem mass spectrometry	350:400	logically derived sequence tandem mass spectrometry (LODES/MSn)	350:412	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	40	theme	spectrometry	329:340	arg1	method					342:347	a mass spectrometry method	322:347	a mass spectrometry method	322:347	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	2	41	theme	N-glycans	225:233	arg1	importance					211:220	the importance	207:220	the importance of N-glycans	207:233	Despite the importance of N-glycans, the structural determination of N-glycan isomers remains challenging.					
36697781	3	42	theme	sequence	368:375	arg1	spectrometry					389:400	logically derived sequence tandem mass spectrometry	350:400	logically derived sequence tandem mass spectrometry (LODES/MSn)	350:412	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	42	theme	sequence	368:375	arg1	method					342:347	a mass spectrometry method	322:347	a mass spectrometry method	322:347	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	42	theme	sequence	368:375	arg1	LODES/MSn					403:411	LODES/MSn	403:411	LODES/MSn	403:411	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	43	theme	derived	360:366	arg1	spectrometry					389:400	logically derived sequence tandem mass spectrometry	350:400	logically derived sequence tandem mass spectrometry (LODES/MSn)	350:412	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	43	theme	derived	360:366	arg1	method					342:347	a mass spectrometry method	322:347	a mass spectrometry method	322:347	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	3	43	theme	derived	360:366	arg1	LODES/MSn					403:411	LODES/MSn	403:411	LODES/MSn	403:411	Here we develop a mass spectrometry method, logically derived sequence tandem mass spectrometry (LODES/MSn), to determine the structures of N-glycan isomers that cannot be determined using conventional mass spectrometry.					
36697781	1	44	theme	important	147:155	arg1	modifications					184:196	the most important protein post-translational modifications	138:196	the most important protein post-translational modifications	138:196	N-linked glycosylation is one of the most important protein post-translational modifications.					
36697781	4	45	theme	ion	683:685	arg1	trap					687:690	an ion trap	680:690	an ion trap for structural determination	680:719	In LODES/MSn, the sequences of successive collision-induced dissociation are derived from carbohydrate dissociation mechanisms and apply to N-glycans in an ion trap for structural determination.					
36697781	0	46	theme	sequence	70:77	arg1	spectrometry					91:102	logically derived sequence tandem mass spectrometry	52:102	logically derived sequence tandem mass spectrometry	52:102	Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry.					
36697781	6	47	theme	oligosaccharides	982:997	arg1	reduction					910:918	reduction	910:918	reduction	910:918	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	6	47	theme	oligosaccharides	982:997	arg1	labeling					925:932	labeling	925:932	labeling of N-glycans	925:945	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	6	47	theme	oligosaccharides	982:997	arg1	permethylation					894:907	permethylation	894:907	permethylation	894:907	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	6	47	theme	oligosaccharides	982:997	arg1	databases					969:977	the mass spectrum databases	951:977	the mass spectrum databases of oligosaccharides and N-glycan standards	951:1020	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	2	48	theme	isomers	277:283	arg1	determination					251:263	the structural determination	236:263	the structural determination of N-glycan isomers	236:283	Despite the importance of N-glycans, the structural determination of N-glycan isomers remains challenging.					
36697781	1	49	theme	protein	157:163	arg1	modifications					184:196	the most important protein post-translational modifications	138:196	the most important protein post-translational modifications	138:196	N-linked glycosylation is one of the most important protein post-translational modifications.					
36697781	6	50	theme	standards	1012:1020	arg1	reduction					910:918	reduction	910:918	reduction	910:918	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	6	50	theme	standards	1012:1020	arg1	labeling					925:932	labeling	925:932	labeling of N-glycans	925:945	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	6	50	theme	standards	1012:1020	arg1	permethylation					894:907	permethylation	894:907	permethylation	894:907	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
36697781	6	50	theme	standards	1012:1020	arg1	databases					969:977	the mass spectrum databases	951:977	the mass spectrum databases of oligosaccharides and N-glycan standards	951:1020	Our method does not require permethylation, reduction, and labeling of N-glycans, or the mass spectrum databases of oligosaccharides and N-glycan standards.					
33966850	3	0	theme	flexibility-morphology	509:530	arg1	relations					532:540	the flexibility-morphology relations	505:540	the flexibility-morphology relations of nanocelluloses in polymer	505:569	Viscoelasticity/elastoplasticity were used as probes to detect the flexibility-morphology relations of nanocelluloses in polymer.					
33966850	4	1	from	polymer	628:634	arg1	rigid					619:623	rigid	619:623	rigid	619:623	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	7	2	from	nanocelluloses	1235:1248	arg1	composites					1261:1270	polymer composites	1253:1270	polymer composites	1253:1270	This work provides interesting information around regulating the dispersion of nanocelluloses in polymer composites by tailoring aspect ratios of nanocelluloses.					
33966850	5	3	theme	elastoplastic	930:942	arg1	deformation					944:954	elastoplastic deformation	930:954	elastoplastic deformation	930:954	Owing to these differences, the viscoelastic flow and elastoplastic deformation of three kinds of composites differed from one another.					
33966850	6	4	theme	work-scaling	1046:1057	arg1	behaviors					1059:1067	The strain-scaling and hysteresis work-scaling behaviors	1012:1067	The strain-scaling and hysteresis work-scaling behaviors	1012:1067	The strain-scaling and hysteresis work-scaling behaviors were then used to establish relaxation scale-structure correlations of target samples.					
33966850	2	5	used	used	358:361	arg2	types					296:300	Three types	290:300	Three types of nanocelluloses with various fiber aspect ratios	290:351	Three types of nanocelluloses with various fiber aspect ratios were used to prepare target composite samples with poly(β-hydroxybutyrate) in this work.					
33966850	0	6	theme	composites	91:100	arg1	viscoelasticity					72:86	viscoelasticity	72:86	viscoelasticity of composites and aspect ratios of nanofibers	72:132	Cellulosic nanofibers filled poly(β-hydroxybutyrate): Relations between viscoelasticity of composites and aspect ratios of nanofibers.					
33966850	4	7	theme	flexible	725:732	arg1	celluloses					687:696	bacterial celluloses	677:696	bacterial celluloses (aspect ratio = 600) fully flexible	677:732	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	4	7	theme	flexible	725:732	arg1	aspect					699:704	aspect ratio = 600	699:716	aspect ratio = 600	699:716	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	2	8	with	nanocelluloses	305:318	arg1	ratios					346:351	various fiber aspect ratios	325:351	various fiber aspect ratios	325:351	Three types of nanocelluloses with various fiber aspect ratios were used to prepare target composite samples with poly(β-hydroxybutyrate) in this work.					
33966850	0	9	theme	ratios	113:118	arg1	viscoelasticity					72:86	viscoelasticity	72:86	viscoelasticity of composites and aspect ratios of nanofibers	72:132	Cellulosic nanofibers filled poly(β-hydroxybutyrate): Relations between viscoelasticity of composites and aspect ratios of nanofibers.					
33966850	7	10	from	composites	1261:1270	arg1	dispersion					1221:1230	the dispersion	1217:1230	the dispersion of nanocelluloses in polymer composites	1217:1270	This work provides interesting information around regulating the dispersion of nanocelluloses in polymer composites by tailoring aspect ratios of nanocelluloses.					
33966850	6	11	theme	scale-structure	1108:1122	arg1	correlations					1124:1135	relaxation scale-structure correlations	1097:1135	relaxation scale-structure correlations of target samples	1097:1153	The strain-scaling and hysteresis work-scaling behaviors were then used to establish relaxation scale-structure correlations of target samples.					
33966850	4	12	dep	semi-flexible	817:829	arg1	aspect					798:803	aspect ratio = 70	798:814	aspect ratio = 70	798:814	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	1	13	theme	fibrous	167:173	arg1	reinforcements					204:217	reinforcements	204:217	reinforcements for polymers, which is highly dependent on geometry of nanocelluloses	204:287	Dispersion states are vital for fibrous nanocelluloses to be used as reinforcements for polymers, which is highly dependent on geometry of nanocelluloses.					
33966850	1	13	theme	fibrous	167:173	arg1	nanocelluloses					175:188	fibrous nanocelluloses	167:188	fibrous nanocelluloses	167:188	Dispersion states are vital for fibrous nanocelluloses to be used as reinforcements for polymers, which is highly dependent on geometry of nanocelluloses.					
33966850	0	14	theme	aspect	106:111	arg1	ratios					113:118	aspect ratios	106:118	aspect ratios	106:118	Cellulosic nanofibers filled poly(β-hydroxybutyrate): Relations between viscoelasticity of composites and aspect ratios of nanofibers.					
33966850	7	15	theme	interesting	1175:1185	arg1	information					1187:1197	interesting information	1175:1197	interesting information around regulating the dispersion of nanocelluloses in polymer composites by tailoring aspect ratios of nanocelluloses	1175:1315	This work provides interesting information around regulating the dispersion of nanocelluloses in polymer composites by tailoring aspect ratios of nanocelluloses.					
33966850	2	16	theme	various	325:331	arg1	ratios					346:351	various fiber aspect ratios	325:351	various fiber aspect ratios	325:351	Three types of nanocelluloses with various fiber aspect ratios were used to prepare target composite samples with poly(β-hydroxybutyrate) in this work.					
33966850	4	17	theme	bacterial	677:685	arg1	celluloses					687:696	bacterial celluloses	677:696	bacterial celluloses (aspect ratio = 600) fully flexible	677:732	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	4	17	theme	bacterial	677:685	arg1	aspect					699:704	aspect ratio = 600	699:716	aspect ratio = 600	699:716	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	6	18	used	used	1079:1082	arg2	behaviors					1059:1067	The strain-scaling and hysteresis work-scaling behaviors	1012:1067	The strain-scaling and hysteresis work-scaling behaviors	1012:1067	The strain-scaling and hysteresis work-scaling behaviors were then used to establish relaxation scale-structure correlations of target samples.					
33966850	5	19	theme	composites	974:983	arg1	kinds					965:969	three kinds	959:969	three kinds of composites	959:983	Owing to these differences, the viscoelastic flow and elastoplastic deformation of three kinds of composites differed from one another.					
33966850	2	20	theme	composite	381:389	arg1	samples					391:397	target composite samples	374:397	target composite samples	374:397	Three types of nanocelluloses with various fiber aspect ratios were used to prepare target composite samples with poly(β-hydroxybutyrate) in this work.					
33966850	0	21	theme	Cellulosic	0:9	arg1	nanofibers					11:20	Cellulosic nanofibers	0:20	Cellulosic nanofibers	0:20	Cellulosic nanofibers filled poly(β-hydroxybutyrate): Relations between viscoelasticity of composites and aspect ratios of nanofibers.					
33966850	6	22	theme	strain-scaling	1016:1029	arg1	behaviors					1059:1067	The strain-scaling and hysteresis work-scaling behaviors	1012:1067	The strain-scaling and hysteresis work-scaling behaviors	1012:1067	The strain-scaling and hysteresis work-scaling behaviors were then used to establish relaxation scale-structure correlations of target samples.					
33966850	2	23	theme	nanocelluloses	305:318	arg1	types					296:300	Three types	290:300	Three types of nanocelluloses with various fiber aspect ratios	290:351	Three types of nanocelluloses with various fiber aspect ratios were used to prepare target composite samples with poly(β-hydroxybutyrate) in this work.					
33966850	5	24	dep	one	999:1001	arg1	another					1003:1009	another	1003:1009	another	1003:1009	Owing to these differences, the viscoelastic flow and elastoplastic deformation of three kinds of composites differed from one another.					
33966850	2	25	theme	target	374:379	arg1	samples					391:397	target composite samples	374:397	target composite samples	374:397	Three types of nanocelluloses with various fiber aspect ratios were used to prepare target composite samples with poly(β-hydroxybutyrate) in this work.					
33966850	7	26	theme	polymer	1253:1259	arg1	composites					1261:1270	polymer composites	1253:1270	polymer composites	1253:1270	This work provides interesting information around regulating the dispersion of nanocelluloses in polymer composites by tailoring aspect ratios of nanocelluloses.					
33966850	1	27	theme	nanocelluloses	274:287	arg1	geometry					262:269	geometry	262:269	geometry of nanocelluloses	262:287	Dispersion states are vital for fibrous nanocelluloses to be used as reinforcements for polymers, which is highly dependent on geometry of nanocelluloses.					
33966850	0	28	theme	nanofibers	123:132	arg1	nanofibers					123:132	nanofibers	123:132	nanofibers	123:132	Cellulosic nanofibers filled poly(β-hydroxybutyrate): Relations between viscoelasticity of composites and aspect ratios of nanofibers.					
33966850	0	28	theme	nanofibers	123:132	arg1	composites					91:100	composites	91:100	composites	91:100	Cellulosic nanofibers filled poly(β-hydroxybutyrate): Relations between viscoelasticity of composites and aspect ratios of nanofibers.					
33966850	0	28	theme	nanofibers	123:132	arg1	ratios					113:118	aspect ratios	106:118	aspect ratios	106:118	Cellulosic nanofibers filled poly(β-hydroxybutyrate): Relations between viscoelasticity of composites and aspect ratios of nanofibers.					
33966850	4	29	theme	flocculated	854:864	arg1	clusters					866:873	loosely flocculated clusters	846:873	loosely flocculated clusters	846:873	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	4	30	theme	cellulose	776:784	arg1	nanofibers					786:795	cellulose nanofibers	776:795	cellulose nanofibers (aspect ratio = 70) semi-flexible	776:829	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	3	31	used	used	480:483	arg2	probes					488:493	probes	488:493	probes	488:493	Viscoelasticity/elastoplasticity were used as probes to detect the flexibility-morphology relations of nanocelluloses in polymer.					
33966850	3	31	used	used	480:483	arg2	Viscoelasticity/elastoplasticity					442:473	Viscoelasticity/elastoplasticity	442:473	Viscoelasticity/elastoplasticity	442:473	Viscoelasticity/elastoplasticity were used as probes to detect the flexibility-morphology relations of nanocelluloses in polymer.					
33966850	4	32	theme	rod-like	653:660	arg1	shape					662:666	their rod-like shape	647:666	their rod-like shape	647:666	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	7	33	theme	nanocelluloses	1235:1248	arg1	dispersion					1221:1230	the dispersion	1217:1230	the dispersion of nanocelluloses in polymer composites	1217:1270	This work provides interesting information around regulating the dispersion of nanocelluloses in polymer composites by tailoring aspect ratios of nanocelluloses.					
33966850	4	34	theme	aspect	596:601	arg1	nanocrystals					582:593	Cellulose nanocrystals	572:593	Cellulose nanocrystals (aspect ratio = 8)	572:612	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	4	34	theme	aspect	596:601	arg1	ratio = 8					603:611	aspect ratio = 8	596:611	aspect ratio = 8	596:611	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	4	35	dep	rigid	619:623	arg1	whereas					669:675	whereas	669:675	whereas	669:675	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	5	36	theme	viscoelastic	908:919	arg1	flow					921:924	viscoelastic flow	908:924	viscoelastic flow	908:924	Owing to these differences, the viscoelastic flow and elastoplastic deformation of three kinds of composites differed from one another.					
33966850	6	37	theme	relaxation	1097:1106	arg1	correlations					1124:1135	relaxation scale-structure correlations	1097:1135	relaxation scale-structure correlations of target samples	1097:1153	The strain-scaling and hysteresis work-scaling behaviors were then used to establish relaxation scale-structure correlations of target samples.					
33966850	6	38	theme	target	1140:1145	arg1	samples					1147:1153	target samples	1140:1153	target samples	1140:1153	The strain-scaling and hysteresis work-scaling behaviors were then used to establish relaxation scale-structure correlations of target samples.					
33966850	4	39	theme	forming	735:741	arg1	structure					761:769	forming closely networked structure	735:769	forming closely networked structure	735:769	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	4	40	theme	Cellulose	572:580	arg1	nanocrystals					582:593	Cellulose nanocrystals	572:593	Cellulose nanocrystals (aspect ratio = 8)	572:612	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	4	40	theme	Cellulose	572:580	arg1	ratio = 8					603:611	aspect ratio = 8	596:611	aspect ratio = 8	596:611	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	7	41	from	dispersion	1221:1230	arg1	composites					1261:1270	polymer composites	1253:1270	polymer composites	1253:1270	This work provides interesting information around regulating the dispersion of nanocelluloses in polymer composites by tailoring aspect ratios of nanocelluloses.					
33966850	1	42	used	used	196:199	arg2	reinforcements					204:217	reinforcements	204:217	reinforcements for polymers, which is highly dependent on geometry of nanocelluloses	204:287	Dispersion states are vital for fibrous nanocelluloses to be used as reinforcements for polymers, which is highly dependent on geometry of nanocelluloses.					
33966850	1	42	used	used	196:199	arg2	nanocelluloses					175:188	fibrous nanocelluloses	167:188	fibrous nanocelluloses	167:188	Dispersion states are vital for fibrous nanocelluloses to be used as reinforcements for polymers, which is highly dependent on geometry of nanocelluloses.					
33966850	7	43	theme	nanocelluloses	1302:1315	arg1	ratios					1292:1297	aspect ratios	1285:1297	aspect ratios of nanocelluloses	1285:1315	This work provides interesting information around regulating the dispersion of nanocelluloses in polymer composites by tailoring aspect ratios of nanocelluloses.					
33966850	2	44	theme	aspect	339:344	arg1	ratios					346:351	various fiber aspect ratios	325:351	various fiber aspect ratios	325:351	Three types of nanocelluloses with various fiber aspect ratios were used to prepare target composite samples with poly(β-hydroxybutyrate) in this work.					
33966850	4	45	theme	semi-flexible	817:829	arg1	nanofibers					786:795	cellulose nanofibers	776:795	cellulose nanofibers (aspect ratio = 70) semi-flexible	776:829	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	3	46	from	relations	532:540	arg1	polymer					563:569	polymer	563:569	polymer	563:569	Viscoelasticity/elastoplasticity were used as probes to detect the flexibility-morphology relations of nanocelluloses in polymer.					
33966850	2	47	theme	fiber	333:337	arg1	ratios					346:351	various fiber aspect ratios	325:351	various fiber aspect ratios	325:351	Three types of nanocelluloses with various fiber aspect ratios were used to prepare target composite samples with poly(β-hydroxybutyrate) in this work.					
33966850	5	48	dep	flow	921:924	arg1	the					904:906	the	904:906	the	904:906	Owing to these differences, the viscoelastic flow and elastoplastic deformation of three kinds of composites differed from one another.					
33966850	5	49	theme	kinds	965:969	arg1	flow					921:924	viscoelastic flow	908:924	viscoelastic flow	908:924	Owing to these differences, the viscoelastic flow and elastoplastic deformation of three kinds of composites differed from one another.					
33966850	5	49	theme	kinds	965:969	arg1	deformation					944:954	elastoplastic deformation	930:954	elastoplastic deformation	930:954	Owing to these differences, the viscoelastic flow and elastoplastic deformation of three kinds of composites differed from one another.					
33966850	0	50	dep	filled	22:27	arg1	Relations					54:62	Relations	54:62	Relations between viscoelasticity of composites and aspect ratios of nanofibers	54:132	Cellulosic nanofibers filled poly(β-hydroxybutyrate): Relations between viscoelasticity of composites and aspect ratios of nanofibers.					
33966850	1	51	theme	Dispersion	135:144	arg1	states					146:151	Dispersion states	135:151	Dispersion states	135:151	Dispersion states are vital for fibrous nanocelluloses to be used as reinforcements for polymers, which is highly dependent on geometry of nanocelluloses.					
33966850	4	52	theme	networked	751:759	arg1	structure					761:769	forming closely networked structure	735:769	forming closely networked structure	735:769	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33966850	6	53	theme	samples	1147:1153	arg1	correlations					1124:1135	relaxation scale-structure correlations	1097:1135	relaxation scale-structure correlations of target samples	1097:1153	The strain-scaling and hysteresis work-scaling behaviors were then used to establish relaxation scale-structure correlations of target samples.					
33966850	7	54	theme	aspect	1285:1290	arg1	ratios					1292:1297	aspect ratios	1285:1297	aspect ratios of nanocelluloses	1285:1315	This work provides interesting information around regulating the dispersion of nanocelluloses in polymer composites by tailoring aspect ratios of nanocelluloses.					
33966850	3	55	theme	nanocelluloses	545:558	arg1	relations					532:540	the flexibility-morphology relations	505:540	the flexibility-morphology relations of nanocelluloses in polymer	505:569	Viscoelasticity/elastoplasticity were used as probes to detect the flexibility-morphology relations of nanocelluloses in polymer.					
33966850	4	56	from	rigid	619:623	arg1	polymer					628:634	polymer	628:634	polymer	628:634	Cellulose nanocrystals (aspect ratio = 8) were rigid in polymer, retaining their rod-like shape, whereas bacterial celluloses (aspect ratio = 600) fully flexible, forming closely networked structure, and cellulose nanofibers (aspect ratio = 70) semi-flexible, dispersing as loosely flocculated clusters.					
33895177	6	0	theme	content	1245:1251	arg1	content					1245:1251	supported silver content	1228:1251	supported silver content	1228:1251	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	6	0	theme	content	1245:1251	arg1	amount					1218:1223	the amount	1214:1223	the amount of supported silver content	1214:1251	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	7	1	theme	good	1318:1321	arg1	stability					1323:1331	good stability	1318:1331	good stability	1318:1331	Taken together, the AF1-Ag with good antibacterial activity and good stability has the potential to be applied in the antibacterial field.					
33895177	2	2	theme	microscope	363:372	arg1	results					330:336	The results	326:336	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS)	326:455	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	5	3	theme	present	1011:1017	arg1	work					1019:1022	the present work	1007:1022	the present work	1007:1022	The antibacterial properties of AF1-Ag were evaluated as an example in the present work.					
33895177	0	4	theme	black	79:83	arg1	fungus					85:90	black fungus	79:90	black fungus	79:90	Construction of silver nanoparticles by the triple helical polysaccharide from black fungus and the antibacterial activities.					
33895177	3	5	dep	analysis	774:781	arg1	evaluation					788:797	evaluation	788:797	thermogravimetric analysis (TG) evaluation	756:797	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	2	6	with	AgNPs	469:473	arg1	size					484:487	the size	480:487	the size from 10-25 nm	480:501	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	2	7	theme	electron	354:361	arg1	microscope					363:372	transmission electron microscope	341:372	transmission electron microscope (TEM)	341:378	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	2	7	theme	electron	354:361	arg1	TEM					375:377	TEM	375:377	TEM	375:377	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	7	8	with	AF1-Ag	1274:1279	arg1	stability					1323:1331	good stability	1318:1331	good stability	1318:1331	Taken together, the AF1-Ag with good antibacterial activity and good stability has the potential to be applied in the antibacterial field.					
33895177	7	8	with	AF1-Ag	1274:1279	arg1	activity					1305:1312	good antibacterial activity	1286:1312	good antibacterial activity	1286:1312	Taken together, the AF1-Ag with good antibacterial activity and good stability has the potential to be applied in the antibacterial field.					
33895177	6	9	theme	supported	1228:1236	arg1	content					1245:1251	supported silver content	1228:1251	supported silver content	1228:1251	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	3	10	theme	loaded	658:663	arg1	content					672:678	the loaded silver content	654:678	the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag)	654:729	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	2	11	theme	nanotubes	560:568	arg1	surface					535:541	the surface	531:541	the surface of AF1 dendritic nanotubes	531:568	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	7	12	theme	antibacterial	1291:1303	arg1	activity					1305:1312	good antibacterial activity	1286:1312	good antibacterial activity	1286:1312	Taken together, the AF1-Ag with good antibacterial activity and good stability has the potential to be applied in the antibacterial field.					
33895177	1	13	theme	triple-helix	263:274	arg1	polysaccharide					276:289	the triple-helix polysaccharide	259:289	the triple-helix polysaccharide extracted from black fungus (AF1)	259:323	Size controllable silver nanoparticles (AgNPs) were synthesized in situ on the polysaccharides-based nanotubes, which were formed by the triple-helix polysaccharide extracted from black fungus (AF1).					
33895177	3	14	theme	composites	687:696	arg1	content					672:678	the loaded silver content	654:678	the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag)	654:729	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	2	15	theme	dendritic	550:558	arg1	nanotubes					560:568	AF1 dendritic nanotubes	546:568	AF1 dendritic nanotubes	546:568	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	3	16	theme	thermogravimetric	756:772	arg1	TG					784:785	TG	784:785	TG	784:785	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	3	16	theme	thermogravimetric	756:772	arg1	analysis					774:781	thermogravimetric analysis	756:781	thermogravimetric analysis (TG) evaluation	756:797	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	6	17	theme	amount	1218:1223	arg1	increase					1202:1209	the increase	1198:1209	the increase of the amount of supported silver content	1198:1251	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	6	18	theme	culture	1042:1048	arg1	medium					1050:1055	the culture medium	1038:1055	the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL)	1038:1104	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	6	19	theme	antibacterial	1124:1136	arg1	properties					1138:1147	obvious antibacterial properties	1116:1147	obvious antibacterial properties	1116:1147	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	6	20	theme	AF1-Ag	1076:1081	arg1	few					1069:1071	few	1069:1071	few	1069:1071	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	2	21	theme	electron	390:397	arg1	SEM					411:413	SEM	411:413	SEM	411:413	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	2	21	theme	electron	390:397	arg1	microscope					399:408	scanning electron microscope	381:408	scanning electron microscope (SEM)	381:414	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	0	22	theme	antibacterial	100:112	arg1	activities					114:123	the antibacterial activities	96:123	the antibacterial activities	96:123	Construction of silver nanoparticles by the triple helical polysaccharide from black fungus and the antibacterial activities.					
33895177	5	23	from	example	996:1002	arg1	work					1019:1022	the present work	1007:1022	the present work	1007:1022	The antibacterial properties of AF1-Ag were evaluated as an example in the present work.					
33895177	4	24	theme	smaller	810:816	arg1	size					818:821	the smaller size	806:821	the smaller size of AgNPs	806:830	Thus, the smaller size of AgNPs and higher silver loading content suggest that the composites could be applied in the biomedical field.					
33895177	7	25	theme	good	1286:1289	arg1	activity					1305:1312	good antibacterial activity	1286:1312	good antibacterial activity	1286:1312	Taken together, the AF1-Ag with good antibacterial activity and good stability has the potential to be applied in the antibacterial field.					
33895177	2	26	theme	scanning	381:388	arg1	SEM					411:413	SEM	411:413	SEM	411:413	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	2	26	theme	scanning	381:388	arg1	microscope					399:408	scanning electron microscope	381:408	scanning electron microscope (SEM)	381:414	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	2	27	theme	spectrometer	438:449	arg1	results					330:336	The results	326:336	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS)	326:455	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	6	28	theme	silver	1238:1243	arg1	content					1245:1251	supported silver content	1228:1251	supported silver content	1228:1251	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	2	29	theme	tubular	594:600	arg1	morphology					602:611	their tubular morphology	588:611	their tubular morphology	588:611	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	2	30	theme	dispersive	427:436	arg1	EDS					452:454	EDS	452:454	EDS	452:454	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	2	30	theme	dispersive	427:436	arg1	spectrometer					438:449	energy dispersive spectrometer	420:449	energy dispersive spectrometer (EDS)	420:455	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	3	31	theme	AF1	709:711	arg1	nanotube					713:720	AF1 nanotube	709:720	AF1 nanotube	709:720	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	3	31	theme	AF1	709:711	arg1	composites					687:696	the composites	683:696	the composites (AgNPs and AF1 nanotube, AF1-Ag)	683:729	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	2	32	theme	energy	420:425	arg1	EDS					452:454	EDS	452:454	EDS	452:454	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	2	32	theme	energy	420:425	arg1	spectrometer					438:449	energy dispersive spectrometer	420:449	energy dispersive spectrometer (EDS)	420:455	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	4	33	theme	silver	843:848	arg1	content					858:864	higher silver loading content	836:864	higher silver loading content	836:864	Thus, the smaller size of AgNPs and higher silver loading content suggest that the composites could be applied in the biomedical field.					
33895177	0	34	theme	silver	16:21	arg1	nanoparticles					23:35	silver nanoparticles	16:35	silver nanoparticles	16:35	Construction of silver nanoparticles by the triple helical polysaccharide from black fungus and the antibacterial activities.					
33895177	1	35	theme	black	306:310	arg1	AF1					320:322	AF1	320:322	AF1	320:322	Size controllable silver nanoparticles (AgNPs) were synthesized in situ on the polysaccharides-based nanotubes, which were formed by the triple-helix polysaccharide extracted from black fungus (AF1).					
33895177	1	35	theme	black	306:310	arg1	fungus					312:317	black fungus	306:317	black fungus (AF1)	306:323	Size controllable silver nanoparticles (AgNPs) were synthesized in situ on the polysaccharides-based nanotubes, which were formed by the triple-helix polysaccharide extracted from black fungus (AF1).					
33895177	4	36	theme	higher	836:841	arg1	content					858:864	higher silver loading content	836:864	higher silver loading content	836:864	Thus, the smaller size of AgNPs and higher silver loading content suggest that the composites could be applied in the biomedical field.					
33895177	0	37	from	fungus	85:90	arg1	polysaccharide					59:72	the triple helical polysaccharide	40:72	the triple helical polysaccharide from black fungus	40:90	Construction of silver nanoparticles by the triple helical polysaccharide from black fungus and the antibacterial activities.					
33895177	4	38	theme	biomedical	918:927	arg1	field					929:933	the biomedical field	914:933	the biomedical field	914:933	Thus, the smaller size of AgNPs and higher silver loading content suggest that the composites could be applied in the biomedical field.					
33895177	1	39	theme	polysaccharides-based	205:225	arg1	nanotubes					227:235	the polysaccharides-based nanotubes	201:235	the polysaccharides-based nanotubes	201:235	Size controllable silver nanoparticles (AgNPs) were synthesized in situ on the polysaccharides-based nanotubes, which were formed by the triple-helix polysaccharide extracted from black fungus (AF1).					
33895177	4	40	theme	loading	850:856	arg1	content					858:864	higher silver loading content	836:864	higher silver loading content	836:864	Thus, the smaller size of AgNPs and higher silver loading content suggest that the composites could be applied in the biomedical field.					
33895177	0	41	theme	nanoparticles	23:35	arg1	Construction					0:11	Construction	0:11	Construction of silver nanoparticles by the triple helical polysaccharide from black fungus	0:90	Construction of silver nanoparticles by the triple helical polysaccharide from black fungus and the antibacterial activities.					
33895177	0	41	theme	nanoparticles	23:35	arg1	activities					114:123	the antibacterial activities	96:123	the antibacterial activities	96:123	Construction of silver nanoparticles by the triple helical polysaccharide from black fungus and the antibacterial activities.					
33895177	2	42	theme	microscope	399:408	arg1	results					330:336	The results	326:336	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS)	326:455	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	0	43	theme	helical	51:57	arg1	polysaccharide					59:72	the triple helical polysaccharide	40:72	the triple helical polysaccharide from black fungus	40:90	Construction of silver nanoparticles by the triple helical polysaccharide from black fungus and the antibacterial activities.					
33895177	2	44	theme	AF1	546:548	arg1	nanotubes					560:568	AF1 dendritic nanotubes	546:568	AF1 dendritic nanotubes	546:568	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	2	45	theme	transmission	341:352	arg1	microscope					363:372	transmission electron microscope	341:372	transmission electron microscope (TEM)	341:378	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	2	45	theme	transmission	341:352	arg1	TEM					375:377	TEM	375:377	TEM	375:377	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	0	46	theme	triple	44:49	arg1	polysaccharide					59:72	the triple helical polysaccharide	40:72	the triple helical polysaccharide from black fungus	40:90	Construction of silver nanoparticles by the triple helical polysaccharide from black fungus and the antibacterial activities.					
33895177	3	47	dep	composites	687:696	arg1	AF1-Ag					723:728	AF1-Ag	723:728	AF1-Ag	723:728	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	3	47	dep	composites	687:696	arg1	AgNPs					699:703	AgNPs	699:703	AgNPs	699:703	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	3	47	dep	composites	687:696	arg1	nanotube					713:720	AF1 nanotube	709:720	AF1 nanotube	709:720	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	3	47	dep	composites	687:696	arg1	composites					687:696	the composites	683:696	the composites (AgNPs and AF1 nanotube, AF1-Ag)	683:729	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	6	48	theme	bacteriostasis	1168:1181	arg1	effect					1158:1163	the effect	1154:1163	the effect of bacteriostasis	1154:1181	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	5	49	theme	AF1-Ag	968:973	arg1	example					996:1002	an example	993:1002	an example in the present work	993:1022	The antibacterial properties of AF1-Ag were evaluated as an example in the present work.					
33895177	5	49	theme	AF1-Ag	968:973	arg1	properties					954:963	The antibacterial properties	936:963	The antibacterial properties of AF1-Ag	936:973	The antibacterial properties of AF1-Ag were evaluated as an example in the present work.					
33895177	6	50	theme	obvious	1116:1122	arg1	properties					1138:1147	obvious antibacterial properties	1116:1147	obvious antibacterial properties	1116:1147	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	1	51	theme	Size	126:129	arg1	AgNPs					166:170	AgNPs	166:170	AgNPs	166:170	Size controllable silver nanoparticles (AgNPs) were synthesized in situ on the polysaccharides-based nanotubes, which were formed by the triple-helix polysaccharide extracted from black fungus (AF1).					
33895177	1	51	theme	Size	126:129	arg1	nanoparticles					151:163	Size controllable silver nanoparticles	126:163	Size controllable silver nanoparticles (AgNPs)	126:171	Size controllable silver nanoparticles (AgNPs) were synthesized in situ on the polysaccharides-based nanotubes, which were formed by the triple-helix polysaccharide extracted from black fungus (AF1).					
33895177	7	52	theme	antibacterial	1372:1384	arg1	field					1386:1390	the antibacterial field	1368:1390	the antibacterial field	1368:1390	Taken together, the AF1-Ag with good antibacterial activity and good stability has the potential to be applied in the antibacterial field.					
33895177	5	53	theme	antibacterial	940:952	arg1	example					996:1002	an example	993:1002	an example in the present work	993:1022	The antibacterial properties of AF1-Ag were evaluated as an example in the present work.					
33895177	5	53	theme	antibacterial	940:952	arg1	properties					954:963	The antibacterial properties	936:963	The antibacterial properties of AF1-Ag	936:973	The antibacterial properties of AF1-Ag were evaluated as an example in the present work.					
33895177	1	54	theme	controllable	131:142	arg1	AgNPs					166:170	AgNPs	166:170	AgNPs	166:170	Size controllable silver nanoparticles (AgNPs) were synthesized in situ on the polysaccharides-based nanotubes, which were formed by the triple-helix polysaccharide extracted from black fungus (AF1).					
33895177	1	54	theme	controllable	131:142	arg1	nanoparticles					151:163	Size controllable silver nanoparticles	126:163	Size controllable silver nanoparticles (AgNPs)	126:171	Size controllable silver nanoparticles (AgNPs) were synthesized in situ on the polysaccharides-based nanotubes, which were formed by the triple-helix polysaccharide extracted from black fungus (AF1).					
33895177	4	55	theme	AgNPs	826:830	arg1	size					818:821	the smaller size	806:821	the smaller size of AgNPs	806:830	Thus, the smaller size of AgNPs and higher silver loading content suggest that the composites could be applied in the biomedical field.					
33895177	4	55	theme	AgNPs	826:830	arg1	content					858:864	higher silver loading content	836:864	higher silver loading content	836:864	Thus, the smaller size of AgNPs and higher silver loading content suggest that the composites could be applied in the biomedical field.					
33895177	2	56	from	nm	500:501	arg1	size					484:487	the size	480:487	the size from 10-25 nm	480:501	The results of transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive spectrometer (EDS) proved that AgNPs with the size from 10-25 nm were uniformly dispersed on the surface of AF1 dendritic nanotubes without affecting their tubular morphology.					
33895177	1	57	theme	silver	144:149	arg1	AgNPs					166:170	AgNPs	166:170	AgNPs	166:170	Size controllable silver nanoparticles (AgNPs) were synthesized in situ on the polysaccharides-based nanotubes, which were formed by the triple-helix polysaccharide extracted from black fungus (AF1).					
33895177	1	57	theme	silver	144:149	arg1	nanoparticles					151:163	Size controllable silver nanoparticles	126:163	Size controllable silver nanoparticles (AgNPs)	126:171	Size controllable silver nanoparticles (AgNPs) were synthesized in situ on the polysaccharides-based nanotubes, which were formed by the triple-helix polysaccharide extracted from black fungus (AF1).					
33895177	7	58	contain	has	1333:1335	arg2	potential					1341:1349	the potential to be applied in the antibacterial field	1337:1390	the potential to be applied in the antibacterial field	1337:1390	Taken together, the AF1-Ag with good antibacterial activity and good stability has the potential to be applied in the antibacterial field.					
33895177	7	58	contain	has	1333:1335	arg1	AF1-Ag					1274:1279	the AF1-Ag	1270:1279	the AF1-Ag with good antibacterial activity and good stability	1270:1331	Taken together, the AF1-Ag with good antibacterial activity and good stability has the potential to be applied in the antibacterial field.					
33895177	3	59	theme	tubular	635:641	arg1	structure					643:651	the tubular structure	631:651	the tubular structure	631:651	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	6	60	theme	ω	1088:1088	arg1	%					1089:1089	10% ω%	1084:1089	10% ω%	1084:1089	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	6	61	dep	=	1094:1094	arg1	%					1089:1089	10% ω%	1084:1089	10% ω%	1084:1089	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
33895177	3	62	theme	silver	665:670	arg1	content					672:678	the loaded silver content	654:678	the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag)	654:729	Moreover, due to the tubular structure, the loaded silver content of the composites (AgNPs and AF1 nanotube, AF1-Ag) could reach about 50% by thermogravimetric analysis (TG) evaluation.					
33895177	6	63	theme	%	1086:1086	arg1	%					1089:1089	10% ω%	1084:1089	10% ω%	1084:1089	As expected, the culture medium contained a few of AF1-Ag (10% ω%, c = 50 μg/mL) exhibited obvious antibacterial properties, and the effect of bacteriostasis increased with the increase of the amount of supported silver content.					
34389389	5	0	with	synthesis	992:1000	arg1	step					1024:1027	a pre-activation step	1007:1027	a pre-activation step to demonstrate its robustness	1007:1057	The structural and thermomechanical properties of the one-step synthesis approach were compared to those of a two-step synthesis with a pre-activation step to demonstrate its robustness.					
34389389	3	1	theme	butyl	508:512	arg1	BuGE					530:533	BuGE	530:533	BuGE	530:533	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	3	1	theme	butyl	508:512	arg1	ether					523:527	butyl glycidyl ether	508:527	butyl glycidyl ether (BuGE)	508:534	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	4	2	theme	single	860:865	arg1	step					867:870	a single step	858:870	a single step	858:870	The formed BuGE derivatives were melt processable and can be manufactured into stretchable thermoplastic films through compression molding, which has never been done before with hemicellulose modified in a single step.					
34389389	3	3	theme	glycidyl	514:521	arg1	BuGE					530:533	BuGE	530:533	BuGE	530:533	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	3	3	theme	glycidyl	514:521	arg1	ether					523:527	butyl glycidyl ether	508:527	butyl glycidyl ether (BuGE)	508:534	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	5	4	theme	two-step	983:990	arg1	synthesis					992:1000	a two-step synthesis	981:1000	a two-step synthesis with a pre-activation step to demonstrate its robustness	981:1057	The structural and thermomechanical properties of the one-step synthesis approach were compared to those of a two-step synthesis with a pre-activation step to demonstrate its robustness.					
34389389	6	5	theme	synthesized	1097:1107	arg1	thermoplastic					1112:1124	the one-step synthesized AX thermoplastic	1084:1124	the one-step synthesized AX thermoplastic with 3 mol of BuGE	1084:1143	The strain at break for the one-step synthesized AX thermoplastic with 3 mol of BuGE is ≈200%.					
34389389	7	6	theme	thermoplastic	1252:1264	arg1	polymers					1266:1273	thermoplastic polymers	1252:1273	thermoplastic polymers made entirely of hemicelluloses	1252:1305	These findings suggest that thermoplastic polymers can be composited with hemicelluloses or that thermoplastic polymers made entirely of hemicelluloses can be designed as packaging and stretchable electronics supports.					
34389389	7	6	theme	thermoplastic	1252:1264	arg1	packaging					1326:1334	packaging	1326:1334	packaging	1326:1334	These findings suggest that thermoplastic polymers can be composited with hemicelluloses or that thermoplastic polymers made entirely of hemicelluloses can be designed as packaging and stretchable electronics supports.					
34389389	7	6	theme	thermoplastic	1252:1264	arg1	electronics					1352:1362	stretchable electronics supports	1340:1371	stretchable electronics supports	1340:1371	These findings suggest that thermoplastic polymers can be composited with hemicelluloses or that thermoplastic polymers made entirely of hemicelluloses can be designed as packaging and stretchable electronics supports.					
34389389	5	7	theme	thermomechanical	892:907	arg1	properties					909:918	The structural and thermomechanical properties	873:918	The structural and thermomechanical properties of the one-step synthesis approach	873:953	The structural and thermomechanical properties of the one-step synthesis approach were compared to those of a two-step synthesis with a pre-activation step to demonstrate its robustness.					
34389389	5	8	theme	synthesis	936:944	arg1	approach					946:953	the one-step synthesis approach	923:953	the one-step synthesis approach	923:953	The structural and thermomechanical properties of the one-step synthesis approach were compared to those of a two-step synthesis with a pre-activation step to demonstrate its robustness.					
34389389	7	9	theme	thermoplastic	1183:1195	arg1	polymers					1197:1204	thermoplastic polymers	1183:1204	thermoplastic polymers	1183:1204	These findings suggest that thermoplastic polymers can be composited with hemicelluloses or that thermoplastic polymers made entirely of hemicelluloses can be designed as packaging and stretchable electronics supports.					
34389389	4	10	theme	formed	658:663	arg1	derivatives					670:680	The formed BuGE derivatives	654:680	The formed BuGE derivatives	654:680	The formed BuGE derivatives were melt processable and can be manufactured into stretchable thermoplastic films through compression molding, which has never been done before with hemicellulose modified in a single step.					
34389389	4	10	theme	formed	658:663	arg1	processable					692:702	processable	692:702	processable	692:702	The formed BuGE derivatives were melt processable and can be manufactured into stretchable thermoplastic films through compression molding, which has never been done before with hemicellulose modified in a single step.					
34389389	2	11	theme	melt-based	398:407	arg1	techniques					428:437	industrial melt-based polymer processing techniques	387:437	industrial melt-based polymer processing techniques	387:437	However, because most hemicelluloses are brittle, they can only be processed as a solution and cannot be processed using industrial melt-based polymer processing techniques.					
34389389	6	12	with	thermoplastic	1112:1124	arg1	3 mol					1131:1135	3 mol	1131:1135	3 mol	1131:1135	The strain at break for the one-step synthesized AX thermoplastic with 3 mol of BuGE is ≈200%.					
34389389	2	13	theme	most	283:286	arg1	hemicelluloses					288:301	most hemicelluloses	283:301	most hemicelluloses	283:301	However, because most hemicelluloses are brittle, they can only be processed as a solution and cannot be processed using industrial melt-based polymer processing techniques.					
34389389	6	14	theme	one-step	1088:1095	arg1	thermoplastic					1112:1124	the one-step synthesized AX thermoplastic	1084:1124	the one-step synthesized AX thermoplastic with 3 mol of BuGE	1084:1143	The strain at break for the one-step synthesized AX thermoplastic with 3 mol of BuGE is ≈200%.					
34389389	1	15	theme	value-added	187:197	arg1	materials					199:207	value-added materials	187:207	value-added materials	187:207	Hemicelluloses are regarded as one of the first candidates for the development of value-added materials due to their renewability, abundance, and functionality.					
34389389	5	16	theme	approach	946:953	arg1	properties					909:918	The structural and thermomechanical properties	873:918	The structural and thermomechanical properties of the one-step synthesis approach	873:953	The structural and thermomechanical properties of the one-step synthesis approach were compared to those of a two-step synthesis with a pre-activation step to demonstrate its robustness.					
34389389	1	17	theme	materials	199:207	arg1	development					172:182	the development	168:182	the development of value-added materials due to their renewability, abundance, and functionality	168:263	Hemicelluloses are regarded as one of the first candidates for the development of value-added materials due to their renewability, abundance, and functionality.					
34389389	7	18	theme	stretchable	1340:1350	arg1	polymers					1266:1273	thermoplastic polymers	1252:1273	thermoplastic polymers made entirely of hemicelluloses	1252:1305	These findings suggest that thermoplastic polymers can be composited with hemicelluloses or that thermoplastic polymers made entirely of hemicelluloses can be designed as packaging and stretchable electronics supports.					
34389389	7	18	theme	stretchable	1340:1350	arg1	electronics					1352:1362	stretchable electronics supports	1340:1371	stretchable electronics supports	1340:1371	These findings suggest that thermoplastic polymers can be composited with hemicelluloses or that thermoplastic polymers made entirely of hemicelluloses can be designed as packaging and stretchable electronics supports.					
34389389	3	19	theme	hydroxyl	545:552	arg1	groups					554:559	the hydroxyl groups	541:559	the hydroxyl groups	541:559	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	0	20	theme	arabinoxylan	20:31	arg1	Hydrophobization					0:15	Hydrophobization	0:15	Hydrophobization of arabinoxylan with n-butyl glycidyl ether	0:59	Hydrophobization of arabinoxylan with n-butyl glycidyl ether yields stretchable thermoplastic materials.					
34389389	4	21	theme	stretchable	733:743	arg1	films					759:763	stretchable thermoplastic films	733:763	stretchable thermoplastic films	733:763	The formed BuGE derivatives were melt processable and can be manufactured into stretchable thermoplastic films through compression molding, which has never been done before with hemicellulose modified in a single step.					
34389389	5	22	theme	structural	877:886	arg1	properties					909:918	The structural and thermomechanical properties	873:918	The structural and thermomechanical properties of the one-step synthesis approach	873:953	The structural and thermomechanical properties of the one-step synthesis approach were compared to those of a two-step synthesis with a pre-activation step to demonstrate its robustness.					
34389389	0	23	theme	glycidyl	46:53	arg1	ether					55:59	n-butyl glycidyl ether	38:59	n-butyl glycidyl ether	38:59	Hydrophobization of arabinoxylan with n-butyl glycidyl ether yields stretchable thermoplastic materials.					
34389389	3	24	theme	terminal	637:644	arg1	ethers					646:651	terminal ethers	637:651	terminal ethers	637:651	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	2	25	theme	processing	417:426	arg1	techniques					428:437	industrial melt-based polymer processing techniques	387:437	industrial melt-based polymer processing techniques	387:437	However, because most hemicelluloses are brittle, they can only be processed as a solution and cannot be processed using industrial melt-based polymer processing techniques.					
34389389	0	26	theme	n-butyl	38:44	arg1	ether					55:59	n-butyl glycidyl ether	38:59	n-butyl glycidyl ether	38:59	Hydrophobization of arabinoxylan with n-butyl glycidyl ether yields stretchable thermoplastic materials.					
34389389	3	27	theme	alkoxy	616:621	arg1	alcohols					623:630	alkoxy alcohols	616:630	alkoxy alcohols	616:630	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	3	28	theme	BuGE	588:591	arg1	ring					601:604	the BuGE epoxide ring	584:604	the BuGE epoxide ring	584:604	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	2	29	theme	polymer	409:415	arg1	techniques					428:437	industrial melt-based polymer processing techniques	387:437	industrial melt-based polymer processing techniques	387:437	However, because most hemicelluloses are brittle, they can only be processed as a solution and cannot be processed using industrial melt-based polymer processing techniques.					
34389389	7	30	dep	electronics	1352:1362	arg1	supports					1364:1371	supports	1364:1371	supports	1364:1371	These findings suggest that thermoplastic polymers can be composited with hemicelluloses or that thermoplastic polymers made entirely of hemicelluloses can be designed as packaging and stretchable electronics supports.					
34389389	6	31	theme	BuGE	1140:1143	arg1	thermoplastic					1112:1124	the one-step synthesized AX thermoplastic	1084:1124	the one-step synthesized AX thermoplastic with 3 mol of BuGE	1084:1143	The strain at break for the one-step synthesized AX thermoplastic with 3 mol of BuGE is ≈200%.					
34389389	5	32	theme	pre-activation	1009:1022	arg1	step					1024:1027	a pre-activation step	1007:1027	a pre-activation step to demonstrate its robustness	1007:1057	The structural and thermomechanical properties of the one-step synthesis approach were compared to those of a two-step synthesis with a pre-activation step to demonstrate its robustness.					
34389389	3	33	dep	arabinoxylan	455:466	arg1	AX					469:470	AX	469:470	AX	469:470	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	0	34	with	Hydrophobization	0:15	arg1	ether					55:59	n-butyl glycidyl ether	38:59	n-butyl glycidyl ether	38:59	Hydrophobization of arabinoxylan with n-butyl glycidyl ether yields stretchable thermoplastic materials.					
34389389	5	35	theme	one-step	927:934	arg1	approach					946:953	the one-step synthesis approach	923:953	the one-step synthesis approach	923:953	The structural and thermomechanical properties of the one-step synthesis approach were compared to those of a two-step synthesis with a pre-activation step to demonstrate its robustness.					
34389389	4	36	theme	thermoplastic	745:757	arg1	films					759:763	stretchable thermoplastic films	733:763	stretchable thermoplastic films	733:763	The formed BuGE derivatives were melt processable and can be manufactured into stretchable thermoplastic films through compression molding, which has never been done before with hemicellulose modified in a single step.					
34389389	4	37	theme	compression	773:783	arg1	molding					785:791	compression molding	773:791	compression molding	773:791	The formed BuGE derivatives were melt processable and can be manufactured into stretchable thermoplastic films through compression molding, which has never been done before with hemicellulose modified in a single step.					
34389389	1	38	theme	first	147:151	arg1	candidates					153:162	the first candidates	143:162	the first candidates for the development of value-added materials due to their renewability, abundance, and functionality	143:263	Hemicelluloses are regarded as one of the first candidates for the development of value-added materials due to their renewability, abundance, and functionality.					
34389389	0	39	theme	thermoplastic	80:92	arg1	materials					94:102	stretchable thermoplastic materials	68:102	stretchable thermoplastic materials	68:102	Hydrophobization of arabinoxylan with n-butyl glycidyl ether yields stretchable thermoplastic materials.					
34389389	6	40	theme	AX	1109:1110	arg1	thermoplastic					1112:1124	the one-step synthesized AX thermoplastic	1084:1124	the one-step synthesized AX thermoplastic with 3 mol of BuGE	1084:1143	The strain at break for the one-step synthesized AX thermoplastic with 3 mol of BuGE is ≈200%.					
34389389	3	41	theme	epoxide	593:599	arg1	ring					601:604	the BuGE epoxide ring	584:604	the BuGE epoxide ring	584:604	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	1	42	theme	candidates	153:162	arg1	one					136:138	one	136:138	one	136:138	Hemicelluloses are regarded as one of the first candidates for the development of value-added materials due to their renewability, abundance, and functionality.					
34389389	1	42	theme	candidates	153:162	arg1	Hemicelluloses					105:118	Hemicelluloses	105:118	Hemicelluloses	105:118	Hemicelluloses are regarded as one of the first candidates for the development of value-added materials due to their renewability, abundance, and functionality.					
34389389	1	42	theme	candidates	153:162	arg1	candidates					153:162	the first candidates	143:162	the first candidates for the development of value-added materials due to their renewability, abundance, and functionality	143:263	Hemicelluloses are regarded as one of the first candidates for the development of value-added materials due to their renewability, abundance, and functionality.					
34389389	2	43	theme	industrial	387:396	arg1	techniques					428:437	industrial melt-based polymer processing techniques	387:437	industrial melt-based polymer processing techniques	387:437	However, because most hemicelluloses are brittle, they can only be processed as a solution and cannot be processed using industrial melt-based polymer processing techniques.					
34389389	0	44	theme	stretchable	68:78	arg1	materials					94:102	stretchable thermoplastic materials	68:102	stretchable thermoplastic materials	68:102	Hydrophobization of arabinoxylan with n-butyl glycidyl ether yields stretchable thermoplastic materials.					
34389389	3	45	theme	ring	601:604	arg1	opening					573:579	the opening	569:579	the opening of the BuGE epoxide ring	569:604	In this study, arabinoxylan (AX) was hydrophobized by incorporating butyl glycidyl ether (BuGE) into the hydroxyl groups through the opening of the BuGE epoxide ring, yielding alkoxy alcohols with terminal ethers.					
34389389	4	46	theme	BuGE	665:668	arg1	derivatives					670:680	The formed BuGE derivatives	654:680	The formed BuGE derivatives	654:680	The formed BuGE derivatives were melt processable and can be manufactured into stretchable thermoplastic films through compression molding, which has never been done before with hemicellulose modified in a single step.					
34389389	4	46	theme	BuGE	665:668	arg1	processable					692:702	processable	692:702	processable	692:702	The formed BuGE derivatives were melt processable and can be manufactured into stretchable thermoplastic films through compression molding, which has never been done before with hemicellulose modified in a single step.					
34290702	1	0	theme	death	216:220	arg1	cause					207:211	a leading cause	197:211	a leading cause of death by infectious diseases	197:243	With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases.					
34290702	6	1	theme	TB	1011:1012	arg1	therapy					1014:1020	TB therapy	1011:1020	TB therapy	1011:1020	These findings highlight 2 novel TB-specific antibody changes that track with the resolution of TB and may provide key insights to guide TB therapy.					
34290702	6	2	theme	key	989:991	arg1	insights					993:1000	key insights	989:1000	key insights	989:1000	These findings highlight 2 novel TB-specific antibody changes that track with the resolution of TB and may provide key insights to guide TB therapy.					
34290702	4	3	from	ATB	708:710	arg1	txATB					697:701	txATB	697:701	txATB from ATB	697:710	As expected, di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc differentiated LTBI from ATB, but also discriminated txATB from ATB.					
34290702	3	4	theme	TB	513:514	arg1	LTBI					527:530	LTBI	527:530	LTBI	527:530	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	3	4	theme	TB	513:514	arg1	infection					516:524	latent TB infection	506:524	latent TB infection (LTBI)	506:531	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	1	5	theme	infectious	225:234	arg1	diseases					236:243	infectious diseases	225:243	infectious diseases	225:243	With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases.					
34290702	6	6	theme	antibody	919:926	arg1	changes					928:934	2 novel TB-specific antibody changes	899:934	2 novel TB-specific antibody changes that track with the resolution of TB	899:971	These findings highlight 2 novel TB-specific antibody changes that track with the resolution of TB and may provide key insights to guide TB therapy.					
34290702	4	7	located	found	628:632	arg1	IgG-Fc					637:642	IgG-Fc	637:642	IgG-Fc	637:642	As expected, di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc differentiated LTBI from ATB, but also discriminated txATB from ATB.					
34290702	4	7	located	found	628:632	arg2	structures					595:604	di-galactosylated glycan structures	570:604	di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc	570:642	As expected, di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc differentiated LTBI from ATB, but also discriminated txATB from ATB.					
34290702	2	8	theme	Humoral	246:252	arg1	immunity					254:261	Humoral immunity	246:261	Humoral immunity following TB treatment	246:284	Humoral immunity following TB treatment is largely uncharacterized, and antibody profiling could provide insights into disease resolution.					
34290702	3	9	theme	TB-specific	420:430	arg1	features					447:454	the distinctive TB-specific serum antibody features	404:454	the distinctive TB-specific serum antibody features in active TB disease (ATB)	404:481	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	3	10	theme	latent	506:511	arg1	LTBI					527:530	LTBI	527:530	LTBI	527:530	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	3	10	theme	latent	506:511	arg1	infection					516:524	latent TB infection	506:524	latent TB infection (LTBI)	506:531	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	3	11	theme	serum	432:436	arg1	features					447:454	the distinctive TB-specific serum antibody features	404:454	the distinctive TB-specific serum antibody features in active TB disease (ATB)	404:481	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	1	12	theme	estimated	84:92	arg1	population					112:121	the global population	101:121	the global population infected with Mycobacterium tuberculosis (Mtb)	101:168	With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases.					
34290702	1	12	theme	estimated	84:92	arg1	%					96:96	an estimated 25%	81:96	an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb)	81:168	With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases.					
34290702	2	13	theme	disease	365:371	arg1	resolution					373:382	disease resolution	365:382	disease resolution	365:382	Humoral immunity following TB treatment is largely uncharacterized, and antibody profiling could provide insights into disease resolution.					
34290702	0	14	theme	Antibody	0:7	arg1	Subclass					9:16	Antibody Subclass	0:16	Antibody Subclass	0:16	Antibody Subclass and Glycosylation Shift Following Effective TB Treatment.					
34290702	6	15	theme	TB-specific	907:917	arg1	changes					928:934	2 novel TB-specific antibody changes	899:934	2 novel TB-specific antibody changes that track with the resolution of TB	899:971	These findings highlight 2 novel TB-specific antibody changes that track with the resolution of TB and may provide key insights to guide TB therapy.					
34290702	3	16	theme	distinctive	408:418	arg1	features					447:454	the distinctive TB-specific serum antibody features	404:454	the distinctive TB-specific serum antibody features in active TB disease (ATB)	404:481	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	0	17	theme	Glycosylation	22:34	arg1	Shift					36:40	Glycosylation Shift	22:40	Glycosylation Shift	22:40	Antibody Subclass and Glycosylation Shift Following Effective TB Treatment.					
34290702	6	18	theme	novel	901:905	arg1	changes					928:934	2 novel TB-specific antibody changes	899:934	2 novel TB-specific antibody changes that track with the resolution of TB	899:971	These findings highlight 2 novel TB-specific antibody changes that track with the resolution of TB and may provide key insights to guide TB therapy.					
34290702	4	19	theme	di-galactosylated	570:586	arg1	structures					595:604	di-galactosylated glycan structures	570:604	di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc	570:642	As expected, di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc differentiated LTBI from ATB, but also discriminated txATB from ATB.					
34290702	5	20	theme	novel	753:757	arg1	feature					768:774	a novel antibody feature	751:774	a novel antibody feature that correlated with active disease, elevated in ATB, but significantly diminished after therapy	751:871	Moreover, TB-specific IgG4 emerged as a novel antibody feature that correlated with active disease, elevated in ATB, but significantly diminished after therapy.					
34290702	2	21	theme	TB	273:274	arg1	treatment					276:284	TB treatment	273:284	TB treatment	273:284	Humoral immunity following TB treatment is largely uncharacterized, and antibody profiling could provide insights into disease resolution.					
34290702	3	22	theme	active	459:464	arg1	ATB					478:480	ATB	478:480	ATB	478:480	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	3	22	theme	active	459:464	arg1	disease					469:475	active TB disease	459:475	active TB disease (ATB)	459:481	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	4	23	theme	sialic	615:620	arg1	acid					622:625	sialic acid	615:625	sialic acid	615:625	As expected, di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc differentiated LTBI from ATB, but also discriminated txATB from ATB.					
34290702	3	24	theme	antibody	438:445	arg1	features					447:454	the distinctive TB-specific serum antibody features	404:454	the distinctive TB-specific serum antibody features in active TB disease (ATB)	404:481	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	4	25	theme	glycan	588:593	arg1	structures					595:604	di-galactosylated glycan structures	570:604	di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc	570:642	As expected, di-galactosylated glycan structures (lacking sialic acid) found on IgG-Fc differentiated LTBI from ATB, but also discriminated txATB from ATB.					
34290702	0	26	theme	TB	62:63	arg1	Treatment					65:73	Effective TB Treatment	52:73	Effective TB Treatment	52:73	Antibody Subclass and Glycosylation Shift Following Effective TB Treatment.					
34290702	3	27	theme	treated	536:542	arg1	txATB					549:553	txATB	549:553	txATB	549:553	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	3	27	theme	treated	536:542	arg1	ATB					544:546	treated ATB	536:546	treated ATB (txATB)	536:554	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	3	28	from	features	447:454	arg1	ATB					478:480	ATB	478:480	ATB	478:480	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	3	28	from	features	447:454	arg1	disease					469:475	active TB disease	459:475	active TB disease (ATB)	459:481	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	2	29	theme	antibody	318:325	arg1	profiling					327:335	antibody profiling	318:335	antibody profiling	318:335	Humoral immunity following TB treatment is largely uncharacterized, and antibody profiling could provide insights into disease resolution.					
34290702	0	30	theme	Effective	52:60	arg1	Treatment					65:73	Effective TB Treatment	52:73	Effective TB Treatment	52:73	Antibody Subclass and Glycosylation Shift Following Effective TB Treatment.					
34290702	3	31	theme	TB	466:467	arg1	ATB					478:480	ATB	478:480	ATB	478:480	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	3	31	theme	TB	466:467	arg1	disease					469:475	active TB disease	459:475	active TB disease (ATB)	459:481	Here we focused on the distinctive TB-specific serum antibody features in active TB disease (ATB) and compared them with latent TB infection (LTBI) or treated ATB (txATB).					
34290702	1	32	theme	global	105:110	arg1	population					112:121	the global population	101:121	the global population infected with Mycobacterium tuberculosis (Mtb)	101:168	With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases.					
34290702	1	33	theme	population	112:121	arg1	population					112:121	the global population	101:121	the global population infected with Mycobacterium tuberculosis (Mtb)	101:168	With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases.					
34290702	1	33	theme	population	112:121	arg1	%					96:96	an estimated 25%	81:96	an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb)	81:168	With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases.					
34290702	5	34	dep	correlated	781:790	arg1	elevated					813:820	elevated	813:820	elevated in ATB	813:827	Moreover, TB-specific IgG4 emerged as a novel antibody feature that correlated with active disease, elevated in ATB, but significantly diminished after therapy.					
34290702	5	34	dep	correlated	781:790	arg1	diminished					848:857	diminished	848:857	significantly diminished after therapy	834:871	Moreover, TB-specific IgG4 emerged as a novel antibody feature that correlated with active disease, elevated in ATB, but significantly diminished after therapy.					
34290702	1	35	theme	leading	199:205	arg1	cause					207:211	a leading cause	197:211	a leading cause of death by infectious diseases	197:243	With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases.					
34290702	6	36	theme	TB	970:971	arg1	resolution					956:965	the resolution	952:965	the resolution of TB	952:971	These findings highlight 2 novel TB-specific antibody changes that track with the resolution of TB and may provide key insights to guide TB therapy.					
34290702	5	37	theme	antibody	759:766	arg1	feature					768:774	a novel antibody feature	751:774	a novel antibody feature that correlated with active disease, elevated in ATB, but significantly diminished after therapy	751:871	Moreover, TB-specific IgG4 emerged as a novel antibody feature that correlated with active disease, elevated in ATB, but significantly diminished after therapy.					
34290702	5	38	theme	active	797:802	arg1	disease					804:810	active disease	797:810	active disease	797:810	Moreover, TB-specific IgG4 emerged as a novel antibody feature that correlated with active disease, elevated in ATB, but significantly diminished after therapy.					
34290702	5	39	theme	TB-specific	723:733	arg1	IgG4					735:738	TB-specific IgG4	723:738	TB-specific IgG4	723:738	Moreover, TB-specific IgG4 emerged as a novel antibody feature that correlated with active disease, elevated in ATB, but significantly diminished after therapy.					
34290702	1	40	theme	Mycobacterium	137:149	arg1	Mtb					165:167	Mtb	165:167	Mtb	165:167	With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases.					
34290702	1	40	theme	Mycobacterium	137:149	arg1	tuberculosis					151:162	Mycobacterium tuberculosis	137:162	Mycobacterium tuberculosis (Mtb)	137:168	With an estimated 25% of the global population infected with Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a leading cause of death by infectious diseases.					
32682045	5	0	theme	branched	811:818	arg1	segments					826:833	the branched chain segments	807:833	the branched chain segments of TPSA similar to the TPSA-4 model molecule	807:878	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	4	1	theme	chemical	737:744	arg1	structure					746:754	chemical structure	737:754	chemical structure of TPSA	737:762	To simulate the branched chain segments of TPSA, four model molecules were designed based on chemical structure of TPSA.					
32682045	0	2	theme	related	66:72	arg1	mechanism					74:82	related mechanism	66:82	related mechanism	66:82	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	0	3	from	conformation	6:17	arg1	tea					58:60	green tea	52:60	green tea	52:60	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	0	3	from	conformation	6:17	arg1	mechanism					74:82	related mechanism	66:82	related mechanism	66:82	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	2	4	theme	homogeneous	366:376	arg1	conformation					378:389	a globular homogeneous conformation	355:389	a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses	355:494	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	0	5	theme	α-amylase	87:95	arg1	activity					108:115	α-amylase inhibitory activity	87:115	α-amylase inhibitory activity	87:115	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	7	6	theme	docking	1188:1194	arg1	analysis					1196:1203	molecular docking analysis	1178:1203	molecular docking analysis	1178:1203	The mechanism of α-amylase inhibitory activity of TPSA was simulated by molecular docking analysis.					
32682045	1	7	theme	different	264:272	arg1	weights					284:290	different molecular weights	264:290	different molecular weights	264:290	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	5	8	theme	docking	775:781	arg1	analysis					783:790	Molecular docking analysis	765:790	Molecular docking analysis	765:790	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	6	9	theme	α-amylase	1056:1064	arg1	structure					1043:1051	the structure	1039:1051	the structure of α-amylase	1039:1064	Circular dichroism spectroscopy showed that the structure of α-amylase was not significantly affected by TPSA.					
32682045	1	10	theme	molecular	274:282	arg1	weights					284:290	different molecular weights	264:290	different molecular weights	264:290	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	0	11	theme	activity	108:115	arg1	tea					58:60	green tea	52:60	green tea	52:60	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	0	11	theme	activity	108:115	arg1	mechanism					74:82	related mechanism	66:82	related mechanism	66:82	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	5	12	theme	hydrogen	964:971	arg1	interactions					981:992	hydrogen bonding interactions	964:992	hydrogen bonding interactions	964:992	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	8	13	theme	branched	1210:1217	arg1	segments					1225:1232	The branched chain segments	1206:1232	The branched chain segments of TPSA similar to the TPSA-4 model molecule	1206:1277	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	8	13	theme	branched	1210:1217	arg1	competitor					1305:1314	a potential competitor	1293:1314	a potential competitor to the starch substrate	1293:1338	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	3	14	theme	globular	577:584	arg1	particles					598:606	globular homogeneous particles	577:606	globular homogeneous particles with size	577:616	Observation by transmission electron microscopy confirmed that TPSA occurred as globular homogeneous particles with size in the range of 20-40 nm.					
32682045	8	15	theme	starch	1323:1328	arg1	substrate					1330:1338	the starch substrate	1319:1338	the starch substrate	1319:1338	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	4	16	theme	model	698:702	arg1	molecules					704:712	four model molecules	693:712	four model molecules	693:712	To simulate the branched chain segments of TPSA, four model molecules were designed based on chemical structure of TPSA.					
32682045	0	17	theme	inhibitory	97:106	arg1	activity					108:115	α-amylase inhibitory activity	87:115	α-amylase inhibitory activity	87:115	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	7	18	theme	molecular	1178:1186	arg1	analysis					1196:1203	molecular docking analysis	1178:1203	molecular docking analysis	1178:1203	The mechanism of α-amylase inhibitory activity of TPSA was simulated by molecular docking analysis.					
32682045	3	19	theme	homogeneous	586:596	arg1	particles					598:606	globular homogeneous particles	577:606	globular homogeneous particles with size	577:616	Observation by transmission electron microscopy confirmed that TPSA occurred as globular homogeneous particles with size in the range of 20-40 nm.					
32682045	2	20	theme	parameters	415:424	arg1	analysis					394:401	analysis	394:401	analysis of solution parameters of each fraction using static light scattering and viscosity analyses	394:494	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	2	21	theme	viscosity	477:485	arg1	analyses					487:494	viscosity analyses	477:494	viscosity analyses	477:494	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	5	22	theme	chain	820:824	arg1	segments					826:833	the branched chain segments	807:833	the branched chain segments of TPSA similar to the TPSA-4 model molecule	807:878	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	2	23	theme	hyperbranched	321:333	arg1	TPSA					293:296	TPSA	293:296	TPSA	293:296	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	2	23	theme	hyperbranched	321:333	arg1	polysaccharide					335:348	a hyperbranched polysaccharide	319:348	a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses	319:494	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	2	24	theme	solution	406:413	arg1	parameters					415:424	solution parameters	406:424	solution parameters of each fraction	406:441	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	0	25	theme	Chain	0:4	arg1	conformation					6:17	Chain conformation	0:17	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.	0:116	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	4	26	theme	TPSA	759:762	arg1	structure					746:754	chemical structure	737:754	chemical structure of TPSA	737:762	To simulate the branched chain segments of TPSA, four model molecules were designed based on chemical structure of TPSA.					
32682045	2	27	with	polysaccharide	335:348	arg1	conformation					378:389	a globular homogeneous conformation	355:389	a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses	355:494	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	5	28	theme	TPSA-4	858:863	arg1	molecule					871:878	the TPSA-4 model molecule	854:878	the TPSA-4 model molecule	854:878	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	5	29	theme	preferential	887:898	arg1	binding					900:906	preferential binding	887:906	preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions	887:992	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	8	30	theme	α-amylase	1367:1375	arg1	activity					1355:1362	the activity	1351:1362	the activity of α-amylase	1351:1375	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	5	31	theme	TPSA	838:841	arg1	segments					826:833	the branched chain segments	807:833	the branched chain segments of TPSA similar to the TPSA-4 model molecule	807:878	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	2	32	theme	static	449:454	arg1	scattering					462:471	static light scattering	449:471	static light scattering	449:471	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	2	33	theme	light	456:460	arg1	scattering					462:471	static light scattering	449:471	static light scattering	449:471	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	7	34	theme	TPSA	1156:1159	arg1	activity					1144:1151	α-amylase inhibitory activity	1123:1151	α-amylase inhibitory activity of TPSA	1123:1159	The mechanism of α-amylase inhibitory activity of TPSA was simulated by molecular docking analysis.					
32682045	0	35	from	mechanism	74:82	arg1	polysaccharide					32:45	an acidic polysaccharide	22:45	an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity	22:115	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	0	35	from	mechanism	74:82	arg1	conformation					6:17	Chain conformation	0:17	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.	0:116	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	8	36	theme	similar	1242:1248	arg1	TPSA					1237:1240	TPSA	1237:1240	TPSA similar to the TPSA-4 model molecule	1237:1277	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	0	37	theme	polysaccharide	32:45	arg1	conformation					6:17	Chain conformation	0:17	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.	0:116	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	7	38	theme	inhibitory	1133:1142	arg1	activity					1144:1151	α-amylase inhibitory activity	1123:1151	α-amylase inhibitory activity of TPSA	1123:1159	The mechanism of α-amylase inhibitory activity of TPSA was simulated by molecular docking analysis.					
32682045	1	39	attach	isolated	154:161	arg2	TPSA					148:151	TPSA	148:151	TPSA	148:151	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	1	39	attach	isolated	154:161	arg1	tea					174:176	green tea	168:176	green tea	168:176	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	1	39	attach	isolated	154:161	arg2	polysaccharide					132:145	An acidic tea polysaccharide	118:145	An acidic tea polysaccharide (TPSA) isolated from green tea	118:176	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	8	40	theme	model	1264:1268	arg1	molecule					1270:1277	the TPSA-4 model molecule	1253:1277	the TPSA-4 model molecule	1253:1277	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	2	41	theme	fraction	434:441	arg1	parameters					415:424	solution parameters	406:424	solution parameters of each fraction	406:441	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	0	42	theme	acidic	25:30	arg1	polysaccharide					32:45	an acidic polysaccharide	22:45	an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity	22:115	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	7	43	theme	activity	1144:1151	arg1	mechanism					1110:1118	The mechanism	1106:1118	The mechanism of α-amylase inhibitory activity of TPSA	1106:1159	The mechanism of α-amylase inhibitory activity of TPSA was simulated by molecular docking analysis.					
32682045	7	43	theme	activity	1144:1151	arg1	simulated					1165:1173	simulated	1165:1173	simulated	1165:1173	The mechanism of α-amylase inhibitory activity of TPSA was simulated by molecular docking analysis.					
32682045	4	44	theme	TPSA	687:690	arg1	segments					675:682	the branched chain segments	656:682	the branched chain segments of TPSA	656:690	To simulate the branched chain segments of TPSA, four model molecules were designed based on chemical structure of TPSA.					
32682045	0	45	theme	green	52:56	arg1	tea					58:60	green tea	52:60	green tea	52:60	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	5	46	theme	Molecular	765:773	arg1	analysis					783:790	Molecular docking analysis	765:790	Molecular docking analysis	765:790	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	0	47	from	tea	58:60	arg1	polysaccharide					32:45	an acidic polysaccharide	22:45	an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity	22:115	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	0	47	from	tea	58:60	arg1	conformation					6:17	Chain conformation	0:17	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.	0:116	Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity.					
32682045	2	48	theme	globular	357:364	arg1	conformation					378:389	a globular homogeneous conformation	355:389	a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses	355:494	TPSA was characterized as a hyperbranched polysaccharide with a globular homogeneous conformation by analysis of solution parameters of each fraction using static light scattering and viscosity analyses.					
32682045	5	49	theme	similar	843:849	arg1	TPSA					838:841	TPSA	838:841	TPSA similar to the TPSA-4 model molecule	838:878	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	6	50	theme	dichroism	1004:1012	arg1	spectroscopy					1014:1025	Circular dichroism spectroscopy	995:1025	Circular dichroism spectroscopy	995:1025	Circular dichroism spectroscopy showed that the structure of α-amylase was not significantly affected by TPSA.					
32682045	7	51	theme	α-amylase	1123:1131	arg1	activity					1144:1151	α-amylase inhibitory activity	1123:1151	α-amylase inhibitory activity of TPSA	1123:1159	The mechanism of α-amylase inhibitory activity of TPSA was simulated by molecular docking analysis.					
32682045	3	52	theme	20-40 nm	634:641	arg1	range					625:629	the range	621:629	the range of 20-40 nm	621:641	Observation by transmission electron microscopy confirmed that TPSA occurred as globular homogeneous particles with size in the range of 20-40 nm.					
32682045	1	53	with	fractions	249:257	arg1	weights					284:290	different molecular weights	264:290	different molecular weights	264:290	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	5	54	theme	bonding	973:979	arg1	interactions					981:992	hydrogen bonding interactions	964:992	hydrogen bonding interactions	964:992	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	6	55	theme	Circular	995:1002	arg1	spectroscopy					1014:1025	Circular dichroism spectroscopy	995:1025	Circular dichroism spectroscopy	995:1025	Circular dichroism spectroscopy showed that the structure of α-amylase was not significantly affected by TPSA.					
32682045	8	56	theme	TPSA	1237:1240	arg1	segments					1225:1232	The branched chain segments	1206:1232	The branched chain segments of TPSA similar to the TPSA-4 model molecule	1206:1277	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	8	56	theme	TPSA	1237:1240	arg1	competitor					1305:1314	a potential competitor	1293:1314	a potential competitor to the starch substrate	1293:1338	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	1	57	theme	acidic	121:126	arg1	TPSA					148:151	TPSA	148:151	TPSA	148:151	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	1	57	theme	acidic	121:126	arg1	polysaccharide					132:145	An acidic tea polysaccharide	118:145	An acidic tea polysaccharide (TPSA) isolated from green tea	118:176	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	1	58	theme	precipitation-fractionation	203:229	arg1	method					231:236	a precipitation-fractionation method	201:236	a precipitation-fractionation method into seven fractions with different molecular weights	201:290	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	8	59	theme	TPSA-4	1257:1262	arg1	molecule					1270:1277	the TPSA-4 model molecule	1253:1277	the TPSA-4 model molecule	1253:1277	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	1	60	theme	tea	128:130	arg1	TPSA					148:151	TPSA	148:151	TPSA	148:151	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	1	60	theme	tea	128:130	arg1	polysaccharide					132:145	An acidic tea polysaccharide	118:145	An acidic tea polysaccharide (TPSA) isolated from green tea	118:176	An acidic tea polysaccharide (TPSA) isolated from green tea was fractionated using a precipitation-fractionation method into seven fractions with different molecular weights.					
32682045	8	61	theme	potential	1295:1303	arg1	segments					1225:1232	The branched chain segments	1206:1232	The branched chain segments of TPSA similar to the TPSA-4 model molecule	1206:1277	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	8	61	theme	potential	1295:1303	arg1	competitor					1305:1314	a potential competitor	1293:1314	a potential competitor to the starch substrate	1293:1338	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	3	62	theme	transmission	512:523	arg1	microscopy					534:543	transmission electron microscopy	512:543	transmission electron microscopy	512:543	Observation by transmission electron microscopy confirmed that TPSA occurred as globular homogeneous particles with size in the range of 20-40 nm.					
32682045	8	63	theme	chain	1219:1223	arg1	segments					1225:1232	The branched chain segments	1206:1232	The branched chain segments of TPSA similar to the TPSA-4 model molecule	1206:1277	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	8	63	theme	chain	1219:1223	arg1	competitor					1305:1314	a potential competitor	1293:1314	a potential competitor to the starch substrate	1293:1338	The branched chain segments of TPSA similar to the TPSA-4 model molecule likely act as a potential competitor to the starch substrate to inhibit the activity of α-amylase.					
32682045	3	64	theme	electron	525:532	arg1	microscopy					534:543	transmission electron microscopy	512:543	transmission electron microscopy	512:543	Observation by transmission electron microscopy confirmed that TPSA occurred as globular homogeneous particles with size in the range of 20-40 nm.					
32682045	4	65	theme	chain	669:673	arg1	segments					675:682	the branched chain segments	656:682	the branched chain segments of TPSA	656:690	To simulate the branched chain segments of TPSA, four model molecules were designed based on chemical structure of TPSA.					
32682045	3	66	with	particles	598:606	arg1	size					613:616	size	613:616	size	613:616	Observation by transmission electron microscopy confirmed that TPSA occurred as globular homogeneous particles with size in the range of 20-40 nm.					
32682045	5	67	theme	model	865:869	arg1	molecule					871:878	the TPSA-4 model molecule	854:878	the TPSA-4 model molecule	854:878	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	5	68	theme	TPSA/α-amylase	933:946	arg1	complex					948:954	the TPSA/α-amylase complex	929:954	the TPSA/α-amylase complex	929:954	Molecular docking analysis indicated that the branched chain segments of TPSA similar to the TPSA-4 model molecule showed preferential binding to α-amylase to form the TPSA/α-amylase complex through hydrogen bonding interactions.					
32682045	4	69	theme	branched	660:667	arg1	segments					675:682	the branched chain segments	656:682	the branched chain segments of TPSA	656:690	To simulate the branched chain segments of TPSA, four model molecules were designed based on chemical structure of TPSA.					
32169445	0	0	theme	purple	100:105	arg1	potato					113:118	purple sweet potato	100:118	purple sweet potato	100:118	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	3	1	dep	compositions	727:738	arg1	the					723:725	the	723:725	the	723:725	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	1	2	from	effects	178:184	arg1	inflammation					286:297	inflammation	286:297	inflammation	286:297	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	1	2	from	effects	178:184	arg1	potato					252:257	purple sweet potato	239:257	purple sweet potato	239:257	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	1	2	from	effects	178:184	arg1	histopathology					270:283	colonic histopathology	262:283	colonic histopathology	262:283	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	1	2	from	effects	178:184	arg1	composition					314:324	microbiota composition	303:324	microbiota composition	303:324	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	2	3	dep	cytokines	586:594	arg1	IL-1β					597:601	IL-1β	597:601	IL-1β	597:601	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	2	3	dep	cytokines	586:594	arg1	cytokines					586:594	pro-inflammatory cytokines	569:594	pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α)	569:618	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	2	3	dep	cytokines	586:594	arg1	TNF-α					613:617	TNF-α	613:617	TNF-α	613:617	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	2	3	dep	cytokines	586:594	arg1	IL-6					604:607	IL-6	604:607	IL-6	604:607	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	1	4	theme	sweet	246:250	arg1	potato					252:257	purple sweet potato	239:257	purple sweet potato	239:257	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	5	5	theme	DSS-induced	1139:1149	arg1	colitis					1151:1157	DSS-induced colitis	1139:1157	DSS-induced colitis mice	1139:1162	Taken together, ASPP alleviated colonic inflammation via blocking pro-inflammatory cytokines; meanwhile ASPP could modulate the structure of gut microbiota in DSS-induced colitis mice.					
32169445	0	6	from	properties	18:27	arg1	potato					113:118	purple sweet potato	100:118	purple sweet potato	100:118	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	0	6	from	properties	18:27	arg1	mice					143:146	DSS-induced colitis mice	123:146	DSS-induced colitis mice	123:146	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	2	7	theme	cytokines	586:594	arg1	levels					559:564	the levels	555:564	the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α)	555:618	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	4	8	theme	ulcerative	955:964	arg1	colitis					966:972	ulcerative colitis	955:972	ulcerative colitis (UC)	955:977	It was found that Parasutterella, Desulfovibrio, Lachnospiraceae, Lactobacillus, Erysipelotrichaceae, Bacteroidetes were the key bacteria associated with ulcerative colitis (UC).					
32169445	4	8	theme	ulcerative	955:964	arg1	UC					975:976	UC	975:976	UC	975:976	It was found that Parasutterella, Desulfovibrio, Lachnospiraceae, Lactobacillus, Erysipelotrichaceae, Bacteroidetes were the key bacteria associated with ulcerative colitis (UC).					
32169445	3	9	theme	rRNA	663:666	arg1	sequencing					681:690	16S rRNA phylogenetic sequencing	659:690	16S rRNA phylogenetic sequencing	659:690	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	0	10	theme	sweet	107:111	arg1	potato					113:118	purple sweet potato	100:118	purple sweet potato	100:118	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	3	11	theme	16S	659:661	arg1	sequencing					681:690	16S rRNA phylogenetic sequencing	659:690	16S rRNA phylogenetic sequencing	659:690	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	1	12	from	composition	314:324	arg1	mice					374:377	dextran sulfate sodium (DSS)-induced colitis mice	329:377	dextran sulfate sodium (DSS)-induced colitis mice	329:377	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	2	13	theme	colonic	623:629	arg1	tissue					631:636	colonic tissue	623:636	colonic tissue	623:636	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	1	14	theme	colonic	262:268	arg1	histopathology					270:283	colonic histopathology	262:283	colonic histopathology	262:283	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	0	15	theme	DSS-induced	123:133	arg1	colitis					135:141	DSS-induced colitis	123:141	DSS-induced colitis mice	123:146	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	1	16	theme	-induced	357:364	arg1	colitis					366:372	dextran sulfate sodium (DSS)-induced colitis	329:372	dextran sulfate sodium (DSS)-induced colitis mice	329:377	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	1	17	from	inflammation	286:297	arg1	mice					374:377	dextran sulfate sodium (DSS)-induced colitis mice	329:377	dextran sulfate sodium (DSS)-induced colitis mice	329:377	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	1	18	theme	potential	168:176	arg1	effects					178:184	the potential effects	164:184	the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice	164:377	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	0	19	from	modulation	48:57	arg1	potato					113:118	purple sweet potato	100:118	purple sweet potato	100:118	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	0	19	from	modulation	48:57	arg1	mice					143:146	DSS-induced colitis mice	123:146	DSS-induced colitis mice	123:146	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	3	20	from	compositions	727:738	arg1	mice					795:798	DSS-induced colitis mice	775:798	DSS-induced colitis mice	775:798	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	2	21	theme	immune	440:445	arg1	indices					453:459	the immune organ indices	436:459	the immune organ indices	436:459	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	2	22	theme	organ	447:451	arg1	indices					453:459	the immune organ indices	436:459	the immune organ indices	436:459	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	5	23	theme	microbiota	1125:1134	arg1	structure					1108:1116	the structure	1104:1116	the structure of gut microbiota in DSS-induced colitis mice	1104:1162	Taken together, ASPP alleviated colonic inflammation via blocking pro-inflammatory cytokines; meanwhile ASPP could modulate the structure of gut microbiota in DSS-induced colitis mice.					
32169445	1	24	from	histopathology	270:283	arg1	mice					374:377	dextran sulfate sodium (DSS)-induced colitis mice	329:377	dextran sulfate sodium (DSS)-induced colitis mice	329:377	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	2	25	theme	colonic	495:501	arg1	histopathology					503:516	colonic histopathology	495:516	colonic histopathology in colitis mice	495:532	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	0	26	theme	Anti-inflammatory	0:16	arg1	properties					18:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	3	27	from	functions	744:752	arg1	mice					795:798	DSS-induced colitis mice	775:798	DSS-induced colitis mice	775:798	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	0	28	theme	gut	33:35	arg1	modulation					48:57	gut microbiota modulation	33:57	gut microbiota modulation	33:57	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	4	29	theme	key	926:928	arg1	Parasutterella					819:832	Parasutterella	819:832	Parasutterella	819:832	It was found that Parasutterella, Desulfovibrio, Lachnospiraceae, Lactobacillus, Erysipelotrichaceae, Bacteroidetes were the key bacteria associated with ulcerative colitis (UC).					
32169445	4	29	theme	key	926:928	arg1	bacteria					930:937	the key bacteria	922:937	the key bacteria associated with ulcerative colitis (UC)	922:977	It was found that Parasutterella, Desulfovibrio, Lachnospiraceae, Lactobacillus, Erysipelotrichaceae, Bacteroidetes were the key bacteria associated with ulcerative colitis (UC).					
32169445	1	30	theme	novel	191:195	arg1	ASPP					228:231	ASPP	228:231	ASPP	228:231	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	1	30	theme	novel	191:195	arg1	polysaccharide					212:225	a novel alkali-soluble polysaccharide	189:225	a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato	189:257	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	0	31	theme	colitis	135:141	arg1	mice					143:146	DSS-induced colitis mice	123:146	DSS-induced colitis mice	123:146	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	5	32	theme	gut	1121:1123	arg1	microbiota					1125:1134	gut microbiota	1121:1134	gut microbiota	1121:1134	Taken together, ASPP alleviated colonic inflammation via blocking pro-inflammatory cytokines; meanwhile ASPP could modulate the structure of gut microbiota in DSS-induced colitis mice.					
32169445	1	33	theme	alkali-soluble	197:210	arg1	ASPP					228:231	ASPP	228:231	ASPP	228:231	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	1	33	theme	alkali-soluble	197:210	arg1	polysaccharide					212:225	a novel alkali-soluble polysaccharide	189:225	a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato	189:257	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	2	34	theme	colon	472:476	arg1	length					478:483	colon length	472:483	colon length	472:483	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	5	35	theme	colonic	1012:1018	arg1	inflammation					1020:1031	colonic inflammation	1012:1031	colonic inflammation	1012:1031	Taken together, ASPP alleviated colonic inflammation via blocking pro-inflammatory cytokines; meanwhile ASPP could modulate the structure of gut microbiota in DSS-induced colitis mice.					
32169445	1	36	theme	polysaccharide	212:225	arg1	effects					178:184	the potential effects	164:184	the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice	164:377	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	0	37	theme	microbiota	37:46	arg1	modulation					48:57	gut microbiota modulation	33:57	gut microbiota modulation	33:57	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	1	38	theme	microbiota	303:312	arg1	composition					314:324	microbiota composition	303:324	microbiota composition	303:324	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	1	39	from	potato	252:257	arg1	ASPP					228:231	ASPP	228:231	ASPP	228:231	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	1	39	from	potato	252:257	arg1	polysaccharide					212:225	a novel alkali-soluble polysaccharide	189:225	a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato	189:257	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	1	39	from	potato	252:257	arg1	effects					178:184	the potential effects	164:184	the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice	164:377	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	5	40	from	structure	1108:1116	arg1	mice					1159:1162	DSS-induced colitis mice	1139:1162	DSS-induced colitis mice	1139:1162	Taken together, ASPP alleviated colonic inflammation via blocking pro-inflammatory cytokines; meanwhile ASPP could modulate the structure of gut microbiota in DSS-induced colitis mice.					
32169445	3	41	theme	microbiota	761:770	arg1	functions					744:752	functions	744:752	functions	744:752	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	3	41	theme	microbiota	761:770	arg1	compositions					727:738	compositions	727:738	compositions	727:738	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	2	42	theme	pro-inflammatory	569:584	arg1	IL-1β					597:601	IL-1β	597:601	IL-1β	597:601	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	2	42	theme	pro-inflammatory	569:584	arg1	cytokines					586:594	pro-inflammatory cytokines	569:594	pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α)	569:618	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	2	42	theme	pro-inflammatory	569:584	arg1	TNF-α					613:617	TNF-α	613:617	TNF-α	613:617	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	2	42	theme	pro-inflammatory	569:584	arg1	IL-6					604:607	IL-6	604:607	IL-6	604:607	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	2	43	theme	colitis	521:527	arg1	mice					529:532	colitis mice	521:532	colitis mice	521:532	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	1	44	theme	colitis	366:372	arg1	mice					374:377	dextran sulfate sodium (DSS)-induced colitis mice	329:377	dextran sulfate sodium (DSS)-induced colitis mice	329:377	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32169445	5	45	theme	colitis	1151:1157	arg1	mice					1159:1162	DSS-induced colitis mice	1139:1162	DSS-induced colitis mice	1139:1162	Taken together, ASPP alleviated colonic inflammation via blocking pro-inflammatory cytokines; meanwhile ASPP could modulate the structure of gut microbiota in DSS-induced colitis mice.					
32169445	5	46	theme	pro-inflammatory	1046:1061	arg1	cytokines					1063:1071	pro-inflammatory cytokines	1046:1071	pro-inflammatory cytokines	1046:1071	Taken together, ASPP alleviated colonic inflammation via blocking pro-inflammatory cytokines; meanwhile ASPP could modulate the structure of gut microbiota in DSS-induced colitis mice.					
32169445	3	47	theme	gut	757:759	arg1	microbiota					761:770	gut microbiota	757:770	gut microbiota	757:770	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	2	48	from	histopathology	503:516	arg1	mice					529:532	colitis mice	521:532	colitis mice	521:532	The results indicated that ASPP restored the immune organ indices, increased colon length, improved colonic histopathology in colitis mice as well as inhibited the levels of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) in colonic tissue and serum.					
32169445	0	49	from	potato	113:118	arg1	modulation					48:57	gut microbiota modulation	33:57	gut microbiota modulation	33:57	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	0	49	from	potato	113:118	arg1	properties					18:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	0	49	from	potato	113:118	arg1	polysaccharide					80:93	an alkali-soluble polysaccharide	62:93	an alkali-soluble polysaccharide from purple sweet potato	62:118	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	0	50	theme	polysaccharide	80:93	arg1	modulation					48:57	gut microbiota modulation	33:57	gut microbiota modulation	33:57	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	0	50	theme	polysaccharide	80:93	arg1	properties					18:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	5	51	theme	meanwhile	1074:1082	arg1	ASPP					1084:1087	meanwhile ASPP	1074:1087	meanwhile ASPP	1074:1087	Taken together, ASPP alleviated colonic inflammation via blocking pro-inflammatory cytokines; meanwhile ASPP could modulate the structure of gut microbiota in DSS-induced colitis mice.					
32169445	3	52	theme	DSS-induced	775:785	arg1	colitis					787:793	DSS-induced colitis	775:793	DSS-induced colitis mice	775:798	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	0	53	theme	alkali-soluble	65:78	arg1	polysaccharide					80:93	an alkali-soluble polysaccharide	62:93	an alkali-soluble polysaccharide from purple sweet potato	62:118	Anti-inflammatory properties and gut microbiota modulation of an alkali-soluble polysaccharide from purple sweet potato in DSS-induced colitis mice.					
32169445	3	54	theme	phylogenetic	668:679	arg1	sequencing					681:690	16S rRNA phylogenetic sequencing	659:690	16S rRNA phylogenetic sequencing	659:690	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	3	55	theme	colitis	787:793	arg1	mice					795:798	DSS-induced colitis mice	775:798	DSS-induced colitis mice	775:798	Moreover, 16S rRNA phylogenetic sequencing revealed that ASPP ameliorated the compositions and functions of gut microbiota in DSS-induced colitis mice.					
32169445	1	56	theme	purple	239:244	arg1	potato					252:257	purple sweet potato	239:257	purple sweet potato	239:257	In this study, the potential effects of a novel alkali-soluble polysaccharide (ASPP) from purple sweet potato on colonic histopathology, inflammation and microbiota composition in dextran sulfate sodium (DSS)-induced colitis mice were evaluated.					
32383850	1	0	theme	blocks	178:183	arg1	assembly					147:154	The spontaneous assembly	131:154	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes	131:223	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	1	0	theme	blocks	178:183	arg1	requirement					234:244	a key requirement	228:244	a key requirement for the structuration of synthetic protocells	228:290	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	1	1	theme	protocells	281:290	arg1	structuration					254:266	the structuration	250:266	the structuration of synthetic protocells	250:290	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	5	2	theme	reversible	1269:1278	arg1	adhesion					1296:1303	reversible lectin-mediated adhesion	1269:1303	reversible lectin-mediated adhesion between the protocells	1269:1326	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	2	3	theme	complex	395:401	arg1	entities					413:420	more complex cell-like entities	390:420	more complex cell-like entities with adaptive membrane properties	390:454	Engineering the functionality and programmability of these building units provides a step toward more complex cell-like entities with adaptive membrane properties.					
32383850	0	4	theme	Synthetic	109:117	arg1	Protocells					119:128	Synthetic Protocells	109:128	Synthetic Protocells	109:128	Lectin-Glycan-Mediated Nanoparticle Docking as a Step toward Programmable Membrane Catalysis and Adhesion in Synthetic Protocells.					
32383850	5	5	theme	cascade	1088:1094	arg1	reaction					1096:1103	a membrane-gated spatially localized cascade reaction	1051:1103	a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane	1051:1192	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	4	6	theme	bio-orthogonal	791:804	arg1	sites					832:836	intrinsic bio-orthogonal lectin-glycan recognition sites	781:836	intrinsic bio-orthogonal lectin-glycan recognition sites	781:836	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	6	7	theme	cell-like	1400:1408	arg1	materials					1410:1418	bioinspired cell-like materials	1388:1418	bioinspired cell-like materials	1388:1418	Our results provide a step toward nanoscale engineering of bioinspired cell-like materials and could have longer-term applications in synthetic virology, protobiology, and microbiosensor and microbioreactor technologies.					
32383850	6	8	theme	bioinspired	1388:1398	arg1	materials					1410:1418	bioinspired cell-like materials	1388:1418	bioinspired cell-like materials	1388:1418	Our results provide a step toward nanoscale engineering of bioinspired cell-like materials and could have longer-term applications in synthetic virology, protobiology, and microbiosensor and microbioreactor technologies.					
32383850	4	9	theme	nanoparticles	925:937	arg1	docking					879:885	the reversible noncovalent docking	852:885	the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes	852:964	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	2	10	theme	cell-like	403:411	arg1	entities					413:420	more complex cell-like entities	390:420	more complex cell-like entities with adaptive membrane properties	390:454	Engineering the functionality and programmability of these building units provides a step toward more complex cell-like entities with adaptive membrane properties.					
32383850	3	11	theme	cellular	535:542	arg1	recognition					544:554	cellular recognition	535:554	cellular recognition	535:554	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	6	12	theme	nanoscale	1363:1371	arg1	engineering					1373:1383	nanoscale engineering	1363:1383	nanoscale engineering of bioinspired cell-like materials	1363:1418	Our results provide a step toward nanoscale engineering of bioinspired cell-like materials and could have longer-term applications in synthetic virology, protobiology, and microbiosensor and microbioreactor technologies.					
32383850	5	13	theme	nanoparticles	1023:1035	arg1	attachment					995:1004	the programmed attachment	980:1004	the programmed attachment of enzyme-loaded nanoparticles	980:1035	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	3	14	theme	interactions	519:530	arg1	role					481:484	the central role	469:484	the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion	469:567	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	3	15	theme	semipermeable	583:595	arg1	microcapsules					620:632	semipermeable polysaccharide-polymer microcapsules	583:632	semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties	583:695	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	3	15	theme	semipermeable	583:595	arg1	polysaccharidosomes					635:653	polysaccharidosomes	635:653	polysaccharidosomes	635:653	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	4	16	theme	recognition	820:830	arg1	sites					832:836	intrinsic bio-orthogonal lectin-glycan recognition sites	781:836	intrinsic bio-orthogonal lectin-glycan recognition sites	781:836	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	1	17	theme	continuous	190:199	arg1	membranes					215:223	continuous semipermeable membranes	190:223	continuous semipermeable membranes	190:223	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	3	18	theme	polysaccharide-polymer	597:618	arg1	microcapsules					620:632	semipermeable polysaccharide-polymer microcapsules	583:632	semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties	583:695	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	3	18	theme	polysaccharide-polymer	597:618	arg1	polysaccharidosomes					635:653	polysaccharidosomes	635:653	polysaccharidosomes	635:653	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	1	19	theme	semipermeable	201:213	arg1	membranes					215:223	continuous semipermeable membranes	190:223	continuous semipermeable membranes	190:223	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	0	20	theme	Nanoparticle	23:34	arg1	Docking					36:42	Nanoparticle Docking	23:42	Nanoparticle Docking	23:42	Lectin-Glycan-Mediated Nanoparticle Docking as a Step toward Programmable Membrane Catalysis and Adhesion in Synthetic Protocells.					
32383850	0	20	theme	Nanoparticle	23:34	arg1	Step					49:52	a Step	47:52	a Step toward Programmable Membrane Catalysis and Adhesion in Synthetic Protocells	47:128	Lectin-Glycan-Mediated Nanoparticle Docking as a Step toward Programmable Membrane Catalysis and Adhesion in Synthetic Protocells.					
32383850	4	21	theme	polymer	905:911	arg1	docking					879:885	the reversible noncovalent docking	852:885	the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes	852:964	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	6	22	contain	have	1430:1433	arg1	results					1333:1339	Our results	1329:1339	Our results	1329:1339	Our results provide a step toward nanoscale engineering of bioinspired cell-like materials and could have longer-term applications in synthetic virology, protobiology, and microbiosensor and microbioreactor technologies.					
32383850	6	22	contain	have	1430:1433	arg2	applications					1447:1458	longer-term applications	1435:1458	longer-term applications	1435:1458	Our results provide a step toward nanoscale engineering of bioinspired cell-like materials and could have longer-term applications in synthetic virology, protobiology, and microbiosensor and microbioreactor technologies.					
32383850	2	23	theme	membrane	436:443	arg1	properties					445:454	adaptive membrane properties	427:454	adaptive membrane properties	427:454	Engineering the functionality and programmability of these building units provides a step toward more complex cell-like entities with adaptive membrane properties.					
32383850	5	24	theme	programmed	984:993	arg1	attachment					995:1004	the programmed attachment	980:1004	the programmed attachment of enzyme-loaded nanoparticles	980:1035	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	6	25	theme	microbioreactor	1520:1534	arg1	technologies					1536:1547	microbioreactor technologies	1520:1547	microbioreactor technologies	1520:1547	Our results provide a step toward nanoscale engineering of bioinspired cell-like materials and could have longer-term applications in synthetic virology, protobiology, and microbiosensor and microbioreactor technologies.					
32383850	5	26	theme	lectin-mediated	1280:1294	arg1	adhesion					1296:1303	reversible lectin-mediated adhesion	1269:1303	reversible lectin-mediated adhesion between the protocells	1269:1326	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	3	27	with	microcapsules	620:632	arg1	properties					686:695	intrinsic lectin-binding properties	661:695	intrinsic lectin-binding properties	661:695	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	2	28	theme	units	361:365	arg1	programmability					327:341	programmability	327:341	programmability	327:341	Engineering the functionality and programmability of these building units provides a step toward more complex cell-like entities with adaptive membrane properties.					
32383850	2	28	theme	units	361:365	arg1	functionality					309:321	functionality	309:321	functionality	309:321	Engineering the functionality and programmability of these building units provides a step toward more complex cell-like entities with adaptive membrane properties.					
32383850	2	29	theme	adaptive	427:434	arg1	properties					445:454	adaptive membrane properties	427:454	adaptive membrane properties	427:454	Engineering the functionality and programmability of these building units provides a step toward more complex cell-like entities with adaptive membrane properties.					
32383850	4	30	theme	functionalized	890:903	arg1	polymer					905:911	functionalized polymer	890:911	functionalized polymer	890:911	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	2	31	theme	building	352:359	arg1	units					361:365	these building units	346:365	these building units	346:365	Engineering the functionality and programmability of these building units provides a step toward more complex cell-like entities with adaptive membrane properties.					
32383850	3	32	theme	-carbohydrate	505:517	arg1	interactions					519:530	protein (lectin)-carbohydrate interactions	489:530	protein (lectin)-carbohydrate interactions	489:530	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	3	33	theme	intrinsic	661:669	arg1	properties					686:695	intrinsic lectin-binding properties	661:695	intrinsic lectin-binding properties	661:695	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	0	34	from	Adhesion	97:104	arg1	Protocells					119:128	Synthetic Protocells	109:128	Synthetic Protocells	109:128	Lectin-Glycan-Mediated Nanoparticle Docking as a Step toward Programmable Membrane Catalysis and Adhesion in Synthetic Protocells.					
32383850	1	35	theme	key	230:232	arg1	assembly					147:154	The spontaneous assembly	131:154	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes	131:223	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	1	35	theme	key	230:232	arg1	requirement					234:244	a key requirement	228:244	a key requirement for the structuration of synthetic protocells	228:290	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	4	36	theme	reversible	856:865	arg1	docking					879:885	the reversible noncovalent docking	852:885	the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes	852:964	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	4	37	theme	lectin-glycan	806:818	arg1	sites					832:836	intrinsic bio-orthogonal lectin-glycan recognition sites	781:836	intrinsic bio-orthogonal lectin-glycan recognition sites	781:836	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	3	38	theme	lectin	498:503	arg1	interactions					519:530	protein (lectin)-carbohydrate interactions	489:530	protein (lectin)-carbohydrate interactions	489:530	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	3	39	theme	protein	489:495	arg1	interactions					519:530	protein (lectin)-carbohydrate interactions	489:530	protein (lectin)-carbohydrate interactions	489:530	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	3	40	theme	central	473:479	arg1	role					481:484	the central role	469:484	the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion	469:567	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	0	41	from	Catalysis	83:91	arg1	Protocells					119:128	Synthetic Protocells	109:128	Synthetic Protocells	109:128	Lectin-Glycan-Mediated Nanoparticle Docking as a Step toward Programmable Membrane Catalysis and Adhesion in Synthetic Protocells.					
32383850	5	42	theme	packed	1236:1241	arg1	networks					1243:1250	extended closely packed networks	1219:1250	extended closely packed networks	1219:1250	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	0	43	theme	Programmable	61:72	arg1	Catalysis					83:91	Programmable Membrane Catalysis	61:91	Programmable Membrane Catalysis	61:91	Lectin-Glycan-Mediated Nanoparticle Docking as a Step toward Programmable Membrane Catalysis and Adhesion in Synthetic Protocells.					
32383850	5	44	theme	localized	1078:1086	arg1	reaction					1096:1103	a membrane-gated spatially localized cascade reaction	1051:1103	a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane	1051:1192	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	3	45	theme	lectin-binding	671:684	arg1	properties					686:695	intrinsic lectin-binding properties	661:695	intrinsic lectin-binding properties	661:695	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	4	46	theme	noncovalent	867:877	arg1	docking					879:885	the reversible noncovalent docking	852:885	the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes	852:964	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	5	47	theme	membrane-gated	1053:1066	arg1	reaction					1096:1103	a membrane-gated spatially localized cascade reaction	1051:1103	a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane	1051:1192	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	1	48	theme	spontaneous	135:145	arg1	assembly					147:154	The spontaneous assembly	131:154	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes	131:223	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	1	48	theme	spontaneous	135:145	arg1	requirement					234:244	a key requirement	228:244	a key requirement for the structuration of synthetic protocells	228:290	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	5	49	theme	extended	1219:1226	arg1	networks					1243:1250	extended closely packed networks	1219:1250	extended closely packed networks	1219:1250	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	4	50	with	endowed	768:774	arg1	sites					832:836	intrinsic bio-orthogonal lectin-glycan recognition sites	781:836	intrinsic bio-orthogonal lectin-glycan recognition sites	781:836	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	6	51	theme	synthetic	1463:1471	arg1	virology					1473:1480	synthetic virology	1463:1480	synthetic virology	1463:1480	Our results provide a step toward nanoscale engineering of bioinspired cell-like materials and could have longer-term applications in synthetic virology, protobiology, and microbiosensor and microbioreactor technologies.					
32383850	5	52	theme	due	1127:1129	arg1	protocells					1116:1125	the protocells	1112:1125	the protocells due to the thermoresponsiveness of the polysaccharidosome membrane	1112:1192	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	4	53	theme	zeolitic	916:923	arg1	nanoparticles					925:937	zeolitic nanoparticles	916:937	zeolitic nanoparticles	916:937	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	4	54	theme	amphiphilic	708:718	arg1	building					752:759	amphiphilic polysaccharide-polymer membrane building	708:759	amphiphilic polysaccharide-polymer membrane building	708:759	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	6	55	theme	materials	1410:1418	arg1	engineering					1373:1383	nanoscale engineering	1363:1383	nanoscale engineering of bioinspired cell-like materials	1363:1418	Our results provide a step toward nanoscale engineering of bioinspired cell-like materials and could have longer-term applications in synthetic virology, protobiology, and microbiosensor and microbioreactor technologies.					
32383850	4	56	from	docking	879:885	arg1	polysaccharidosomes					946:964	the polysaccharidosomes	942:964	the polysaccharidosomes	942:964	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	5	57	theme	polysaccharidosome	1166:1183	arg1	membrane					1185:1192	the polysaccharidosome membrane	1162:1192	the polysaccharidosome membrane	1162:1192	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	3	58	from	role	481:484	arg1	recognition					544:554	cellular recognition	535:554	cellular recognition	535:554	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	3	58	from	role	481:484	arg1	adhesion					560:567	adhesion	560:567	adhesion	560:567	Inspired by the central role of protein (lectin)-carbohydrate interactions in cellular recognition and adhesion, we fabricate semipermeable polysaccharide-polymer microcapsules (polysaccharidosomes) with intrinsic lectin-binding properties.					
32383850	2	59	dep	functionality	309:321	arg1	the					305:307	the	305:307	the	305:307	Engineering the functionality and programmability of these building units provides a step toward more complex cell-like entities with adaptive membrane properties.					
32383850	6	60	theme	longer-term	1435:1445	arg1	applications					1447:1458	longer-term applications	1435:1458	longer-term applications	1435:1458	Our results provide a step toward nanoscale engineering of bioinspired cell-like materials and could have longer-term applications in synthetic virology, protobiology, and microbiosensor and microbioreactor technologies.					
32383850	2	61	with	entities	413:420	arg1	properties					445:454	adaptive membrane properties	427:454	adaptive membrane properties	427:454	Engineering the functionality and programmability of these building units provides a step toward more complex cell-like entities with adaptive membrane properties.					
32383850	5	62	theme	enzyme-loaded	1009:1021	arg1	nanoparticles					1023:1035	enzyme-loaded nanoparticles	1009:1035	enzyme-loaded nanoparticles	1009:1035	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	1	63	theme	nanoscale	159:167	arg1	blocks					178:183	nanoscale building blocks	159:183	nanoscale building blocks	159:183	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	4	64	theme	intrinsic	781:789	arg1	sites					832:836	intrinsic bio-orthogonal lectin-glycan recognition sites	781:836	intrinsic bio-orthogonal lectin-glycan recognition sites	781:836	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	4	65	theme	membrane	743:750	arg1	building					752:759	amphiphilic polysaccharide-polymer membrane building	708:759	amphiphilic polysaccharide-polymer membrane building	708:759	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	0	66	theme	Membrane	74:81	arg1	Catalysis					83:91	Programmable Membrane Catalysis	61:91	Programmable Membrane Catalysis	61:91	Lectin-Glycan-Mediated Nanoparticle Docking as a Step toward Programmable Membrane Catalysis and Adhesion in Synthetic Protocells.					
32383850	5	67	theme	membrane	1185:1192	arg1	thermoresponsiveness					1138:1157	the thermoresponsiveness	1134:1157	the thermoresponsiveness of the polysaccharidosome membrane	1134:1192	We show that the programmed attachment of enzyme-loaded nanoparticles gives rise to a membrane-gated spatially localized cascade reaction within the protocells due to the thermoresponsiveness of the polysaccharidosome membrane, and we demonstrate that extended closely packed networks are produced via reversible lectin-mediated adhesion between the protocells.					
32383850	4	68	theme	polysaccharide-polymer	720:741	arg1	building					752:759	amphiphilic polysaccharide-polymer membrane building	708:759	amphiphilic polysaccharide-polymer membrane building	708:759	We employ amphiphilic polysaccharide-polymer membrane building blocks endowed with intrinsic bio-orthogonal lectin-glycan recognition sites to facilitate the reversible noncovalent docking of functionalized polymer or zeolitic nanoparticles on the polysaccharidosomes.					
32383850	1	69	theme	building	169:176	arg1	blocks					178:183	nanoscale building blocks	159:183	nanoscale building blocks	159:183	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
32383850	1	70	theme	synthetic	271:279	arg1	protocells					281:290	synthetic protocells	271:290	synthetic protocells	271:290	The spontaneous assembly of nanoscale building blocks into continuous semipermeable membranes is a key requirement for the structuration of synthetic protocells.					
31963790	7	0	theme	therapeutic	1089:1099	arg1	effect					1101:1106	the therapeutic effect	1085:1106	the therapeutic effect	1085:1106	These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.					
31963790	2	1	dep	fractions	193:201	arg1	GUPS-II					212:218	GUPS-II	212:218	GUPS-II	212:218	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	2	1	dep	fractions	193:201	arg1	GUPS-I					204:209	GUPS-I	204:209	GUPS-I	204:209	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	2	1	dep	fractions	193:201	arg1	fractions					193:201	Three fractions	187:201	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS)	187:271	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	2	1	dep	fractions	193:201	arg1	GUPS-III					224:231	GUPS-III	224:231	GUPS-III	224:231	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	0	2	theme	Glycyrrhiza	85:95	arg1	uralensis					97:105	Glycyrrhiza uralensis	85:105	Glycyrrhiza uralensis	85:105	Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis.					
31963790	4	3	theme	microscopy	506:515	arg1	results					477:483	The results	473:483	The results of scanning electron microscopy, FT-IR, and NMR	473:531	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	2	4	theme	kDa	334:336	arg1	weights					302:308	molecular weights	292:308	molecular weights of 1.06, 29.1, and 14.9 kDa	292:336	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	4	5	theme	electron	497:504	arg1	microscopy					506:515	scanning electron microscopy	488:515	scanning electron microscopy	488:515	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	2	6	theme	molecular	292:300	arg1	weights					302:308	molecular weights	292:308	molecular weights of 1.06, 29.1, and 14.9 kDa	292:336	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	6	7	theme	DCs	963:965	arg1	maturation					949:958	the maturation	945:958	the maturation of DCs	945:965	An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs.					
31963790	6	8	dep	in	878:879	arg1	vivo					881:884	vivo	881:884	vivo	881:884	An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs.					
31963790	7	9	contain	has	1004:1006	arg2	potential					1012:1020	the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect	1008:1106	the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect	1008:1106	These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.					
31963790	7	9	contain	has	1004:1006	arg1	GUPS-II					996:1002	GUPS-II	996:1002	GUPS-II	996:1002	These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.					
31963790	4	10	contain	contained	678:686	arg2	linkages					706:713	only α-glycosidic linkages	688:713	only α-glycosidic linkages	688:713	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	4	10	contain	contained	678:686	arg1	GUPS-I					652:657	GUPS-I	652:657	GUPS-I	652:657	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	1	11	with	medicine	150:157	arg1	bioactivities					172:184	various bioactivities	164:184	various bioactivities	164:184	Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities.					
31963790	4	12	theme	β-glycosidic	623:634	arg1	linkages					636:643	α- and β-glycosidic linkages	616:643	α- and β-glycosidic linkages	616:643	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	5	13	theme	better	757:762	arg1	bioactivities					764:776	better bioactivities	757:776	better bioactivities on the maturation and cytokine production of dendritic cells (DCs)	757:843	Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I.					
31963790	5	14	theme	cytokine	800:807	arg1	production					809:818	cytokine production	800:818	cytokine production	800:818	Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I.					
31963790	7	15	theme	cancer	1053:1058	arg1	immunotherapy					1060:1072	cancer immunotherapy	1053:1072	cancer immunotherapy	1053:1072	These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.					
31963790	1	16	theme	Glycyrrhiza	108:118	arg1	medicine					150:157	a Chinese herbal medicine	133:157	a Chinese herbal medicine with various bioactivities	133:184	Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities.					
31963790	1	16	theme	Glycyrrhiza	108:118	arg1	uralensis					120:128	Glycyrrhiza uralensis	108:128	Glycyrrhiza uralensis	108:128	Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities.					
31963790	4	17	theme	NMR	529:531	arg1	results					477:483	The results	473:483	The results of scanning electron microscopy, FT-IR, and NMR	473:531	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	5	18	theme	dendritic	823:831	arg1	DCs					840:842	DCs	840:842	DCs	840:842	Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I.					
31963790	5	18	theme	dendritic	823:831	arg1	cells					833:837	dendritic cells	823:837	dendritic cells (DCs)	823:843	Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I.					
31963790	3	19	theme	monosaccharide	357:370	arg1	compositions					372:383	The monosaccharide compositions	353:383	The monosaccharide compositions of GUPS-II and GUPS-III	353:407	The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different.					
31963790	3	19	theme	monosaccharide	357:370	arg1	similar					414:420	similar	414:420	similar	414:420	The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different.					
31963790	0	20	from	uralensis	97:105	arg1	Characterization					13:28	Characterization	13:28	Characterization	13:28	Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis.					
31963790	0	20	from	uralensis	97:105	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis.					
31963790	0	20	from	uralensis	97:105	arg1	Activity					52:59	Immuno-Enhancing Activity	35:59	Immuno-Enhancing Activity	35:59	Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis.					
31963790	5	21	theme	cells	833:837	arg1	maturation					785:794	maturation	785:794	maturation	785:794	Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I.					
31963790	5	21	theme	cells	833:837	arg1	production					809:818	cytokine production	800:818	cytokine production	800:818	Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I.					
31963790	3	22	theme	GUPS-III	400:407	arg1	compositions					372:383	The monosaccharide compositions	353:383	The monosaccharide compositions of GUPS-II and GUPS-III	353:407	The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different.					
31963790	3	22	theme	GUPS-III	400:407	arg1	similar					414:420	similar	414:420	similar	414:420	The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different.					
31963790	2	23	theme	uralensis	240:248	arg1	GUPS					267:270	GUPS	267:270	GUPS	267:270	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	2	23	theme	uralensis	240:248	arg1	polysaccharides					250:264	G. uralensis polysaccharides	237:264	G. uralensis polysaccharides (GUPS)	237:271	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	4	24	theme	FT-IR	518:522	arg1	results					477:483	The results	473:483	The results of scanning electron microscopy, FT-IR, and NMR	473:531	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	2	25	theme	G.	237:238	arg1	GUPS					267:270	GUPS	267:270	GUPS	267:270	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	2	25	theme	G.	237:238	arg1	polysaccharides					250:264	G. uralensis polysaccharides	237:264	G. uralensis polysaccharides (GUPS)	237:271	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	4	26	theme	α-	616:617	arg1	linkages					636:643	α- and β-glycosidic linkages	616:643	α- and β-glycosidic linkages	616:643	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	3	27	theme	GUPS-II	388:394	arg1	compositions					372:383	The monosaccharide compositions	353:383	The monosaccharide compositions of GUPS-II and GUPS-III	353:407	The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different.					
31963790	3	27	theme	GUPS-II	388:394	arg1	similar					414:420	similar	414:420	similar	414:420	The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different.					
31963790	6	28	theme	only	909:912	arg1	GUPS-II					914:920	only GUPS-II	909:920	only GUPS-II	909:920	An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs.					
31963790	7	29	with	combination	1036:1046	arg1	immunotherapy					1060:1072	cancer immunotherapy	1053:1072	cancer immunotherapy	1053:1072	These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.					
31963790	4	30	contain	contained	606:614	arg2	linkages					636:643	α- and β-glycosidic linkages	616:643	α- and β-glycosidic linkages	616:643	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	4	30	contain	contained	606:614	arg1	GUPS-II					548:554	GUPS-II	548:554	GUPS-II	548:554	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	6	31	theme	in	878:879	arg1	experiment					886:895	An in vivo experiment	875:895	An in vivo experiment	875:895	An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs.					
31963790	5	32	dep	maturation	785:794	arg1	the					781:783	the	781:783	the	781:783	Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I.					
31963790	1	33	theme	Chinese	135:141	arg1	medicine					150:157	a Chinese herbal medicine	133:157	a Chinese herbal medicine with various bioactivities	133:184	Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities.					
31963790	1	33	theme	Chinese	135:141	arg1	uralensis					120:128	Glycyrrhiza uralensis	108:128	Glycyrrhiza uralensis	108:128	Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities.					
31963790	4	34	with	flaky	574:578	arg1	surface					594:600	a smooth surface	585:600	a smooth surface	585:600	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	0	35	theme	Immuno-Enhancing	35:50	arg1	Activity					52:59	Immuno-Enhancing Activity	35:59	Immuno-Enhancing Activity	35:59	Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis.					
31963790	4	36	theme	α-glycosidic	693:704	arg1	linkages					706:713	only α-glycosidic linkages	688:713	only α-glycosidic linkages	688:713	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	1	37	theme	herbal	143:148	arg1	medicine					150:157	a Chinese herbal medicine	133:157	a Chinese herbal medicine with various bioactivities	133:184	Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities.					
31963790	1	37	theme	herbal	143:148	arg1	uralensis					120:128	Glycyrrhiza uralensis	108:128	Glycyrrhiza uralensis	108:128	Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities.					
31963790	5	38	from	bioactivities	764:776	arg1	maturation					785:794	maturation	785:794	maturation	785:794	Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I.					
31963790	5	38	from	bioactivities	764:776	arg1	production					809:818	cytokine production	800:818	cytokine production	800:818	Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I.					
31963790	0	39	theme	Polysaccharides	64:78	arg1	Characterization					13:28	Characterization	13:28	Characterization	13:28	Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis.					
31963790	0	39	theme	Polysaccharides	64:78	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis.					
31963790	0	39	theme	Polysaccharides	64:78	arg1	Activity					52:59	Immuno-Enhancing Activity	35:59	Immuno-Enhancing Activity	35:59	Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis.					
31963790	4	40	theme	scanning	488:495	arg1	microscopy					506:515	scanning electron microscopy	488:515	scanning electron microscopy	488:515	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
31963790	2	41	theme	polysaccharides	250:264	arg1	GUPS-II					212:218	GUPS-II	212:218	GUPS-II	212:218	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	2	41	theme	polysaccharides	250:264	arg1	GUPS-I					204:209	GUPS-I	204:209	GUPS-I	204:209	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	2	41	theme	polysaccharides	250:264	arg1	fractions					193:201	Three fractions	187:201	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS)	187:271	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	2	41	theme	polysaccharides	250:264	arg1	GUPS-III					224:231	GUPS-III	224:231	GUPS-III	224:231	Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively.					
31963790	1	42	theme	various	164:170	arg1	bioactivities					172:184	various bioactivities	164:184	various bioactivities	164:184	Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities.					
31963790	4	43	theme	smooth	587:592	arg1	surface					594:600	a smooth surface	585:600	a smooth surface	585:600	The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages.					
32036182	8	0	contain	have	1304:1307	arg2	potential					1309:1317	potential	1309:1317	potential	1309:1317	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	8	0	contain	have	1304:1307	arg1	DFs					1295:1297	DFs	1295:1297	DFs that have potential to improve large bowel function	1295:1349	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	3	1	theme	hazelnut	376:383	arg1	contents					356:363	Total DF contents	347:363	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin	347:420	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin were 17.8, 15.4, and 69.8%, respectively; majority of which (>96%) were water-insoluble.					
32036182	2	2	theme	chromatography-mass	305:323	arg1	GC-MS					339:343	GC-MS	339:343	GC-MS	339:343	Insoluble (IDF) and soluble (SDF) DFs were examined for monosaccharide and glycosyl-linkage compositions using gas chromatography-mass spectrometry (GC-MS).					
32036182	2	2	theme	chromatography-mass	305:323	arg1	spectrometry					325:336	gas chromatography-mass spectrometry	301:336	gas chromatography-mass spectrometry (GC-MS)	301:344	Insoluble (IDF) and soluble (SDF) DFs were examined for monosaccharide and glycosyl-linkage compositions using gas chromatography-mass spectrometry (GC-MS).					
32036182	5	3	dep	arabinan	950:957	arg1	chains					977:982	side chains	972:982	side chains	972:982	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	8	4	contain	having	1477:1482	arg1	carbohydrates					1463:1475	functional carbohydrates	1452:1475	functional carbohydrates having prebiotic capacities	1452:1503	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	8	4	contain	having	1477:1482	arg2	capacities					1494:1503	prebiotic capacities	1484:1503	prebiotic capacities	1484:1503	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	1	5	theme	natural	122:128	arg1	hazelnut					130:137	natural hazelnut	122:137	natural hazelnut	122:137	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin were analyzed.					
32036182	0	6	theme	hazelnut	76:83	arg1	profiles					14:21	Dietary fibre profiles	0:21	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.	0:89	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.					
32036182	2	7	theme	gas	301:303	arg1	GC-MS					339:343	GC-MS	339:343	GC-MS	339:343	Insoluble (IDF) and soluble (SDF) DFs were examined for monosaccharide and glycosyl-linkage compositions using gas chromatography-mass spectrometry (GC-MS).					
32036182	2	7	theme	gas	301:303	arg1	spectrometry					325:336	gas chromatography-mass spectrometry	301:336	gas chromatography-mass spectrometry (GC-MS)	301:344	Insoluble (IDF) and soluble (SDF) DFs were examined for monosaccharide and glycosyl-linkage compositions using gas chromatography-mass spectrometry (GC-MS).					
32036182	3	8	theme	which	476:480	arg1	majority					464:471	majority	464:471	majority of which (>96%)	464:487	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin were 17.8, 15.4, and 69.8%, respectively; majority of which (>96%) were water-insoluble.					
32036182	8	9	theme	hazelnut	1385:1392	arg1	process					1403:1409	hazelnut roasting process	1385:1409	hazelnut roasting process	1385:1409	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	5	10	theme	higher	886:891	arg1	proportion					893:902	higher proportion	886:902	higher proportion of hairy region that is heavily branched with arabinan and galactan side chains	886:982	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	7	11	theme	monomeric	1219:1227	arg1	chains					1234:1239	monomeric side chains	1219:1239	monomeric side chains	1219:1239	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	8	12	theme	bowel	1336:1340	arg1	function					1342:1349	large bowel function	1330:1349	large bowel function	1330:1349	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	3	13	theme	skin	417:420	arg1	contents					356:363	Total DF contents	347:363	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin	347:420	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin were 17.8, 15.4, and 69.8%, respectively; majority of which (>96%) were water-insoluble.					
32036182	2	14	dep	soluble	210:216	arg1	SDF					219:221	SDF	219:221	SDF	219:221	Insoluble (IDF) and soluble (SDF) DFs were examined for monosaccharide and glycosyl-linkage compositions using gas chromatography-mass spectrometry (GC-MS).					
32036182	1	15	theme	hazelnut	130:137	arg1	profiles					110:117	Dietary fibre (DF) profiles	91:117	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin	91:173	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin were analyzed.					
32036182	7	16	theme	side	1229:1232	arg1	chains					1234:1239	monomeric side chains	1219:1239	monomeric side chains	1219:1239	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	8	17	from	DFs	1295:1297	arg1	rich					1287:1290	rich	1287:1290	rich	1287:1290	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	3	18	theme	Total	347:351	arg1	contents					356:363	Total DF contents	347:363	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin	347:420	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin were 17.8, 15.4, and 69.8%, respectively; majority of which (>96%) were water-insoluble.					
32036182	8	19	theme	carbohydrates	1463:1475	arg1	production					1438:1447	the production	1434:1447	the production of functional carbohydrates having prebiotic capacities	1434:1503	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	3	20	theme	hazelnut	394:401	arg1	contents					356:363	Total DF contents	347:363	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin	347:420	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin were 17.8, 15.4, and 69.8%, respectively; majority of which (>96%) were water-insoluble.					
32036182	0	21	theme	fibre	8:12	arg1	profiles					14:21	Dietary fibre profiles	0:21	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.	0:89	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.					
32036182	5	22	theme	hairy	907:911	arg1	region					913:918	hairy region	907:918	hairy region that is heavily branched with arabinan and galactan side chains	907:982	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	6	23	theme	side	1054:1057	arg1	chains					1059:1064	monomeric and/or dimeric side chains	1029:1064	monomeric and/or dimeric side chains	1029:1064	Xyloglucans were also densely branched with monomeric and/or dimeric side chains.					
32036182	2	24	theme	monosaccharide	246:259	arg1	compositions					282:293	monosaccharide and glycosyl-linkage compositions	246:293	compositions	282:293	Insoluble (IDF) and soluble (SDF) DFs were examined for monosaccharide and glycosyl-linkage compositions using gas chromatography-mass spectrometry (GC-MS).					
32036182	8	25	theme	functional	1452:1461	arg1	carbohydrates					1463:1475	functional carbohydrates	1452:1475	functional carbohydrates having prebiotic capacities	1452:1503	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	1	26	theme	roasted	140:146	arg1	hazelnut					148:155	roasted hazelnut	140:155	roasted hazelnut	140:155	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin were analyzed.					
32036182	0	27	theme	Dietary	0:6	arg1	profiles					14:21	Dietary fibre profiles	0:21	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.	0:89	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.					
32036182	6	28	theme	dimeric	1046:1052	arg1	chains					1059:1064	monomeric and/or dimeric side chains	1029:1064	monomeric and/or dimeric side chains	1029:1064	Xyloglucans were also densely branched with monomeric and/or dimeric side chains.					
32036182	8	29	from	rich	1287:1290	arg1	DFs					1295:1297	DFs	1295:1297	DFs that have potential to improve large bowel function	1295:1349	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	5	30	from	polysaccharides	820:834	arg1	IDFs					839:842	IDFs	839:842	IDFs	839:842	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	1	31	theme	hazelnut	148:155	arg1	profiles					110:117	Dietary fibre (DF) profiles	91:117	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin	91:173	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin were analyzed.					
32036182	5	32	theme	side	972:975	arg1	chains					977:982	side chains	972:982	side chains	972:982	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	8	33	used	utilized	1421:1428	arg2	byproduct					1372:1380	a byproduct	1370:1380	a byproduct of hazelnut roasting process	1370:1409	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	8	33	used	utilized	1421:1428	arg2	skin					1364:1367	hazelnut skin	1355:1367	hazelnut skin	1355:1367	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	3	34	theme	hazelnut	408:415	arg1	skin					417:420	hazelnut skin	408:420	hazelnut skin	408:420	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin were 17.8, 15.4, and 69.8%, respectively; majority of which (>96%) were water-insoluble.					
32036182	4	35	theme	pectic	729:734	arg1	polysaccharides					736:750	pectic polysaccharides	729:750	pectic polysaccharides	729:750	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	4	35	theme	pectic	729:734	arg1	polysaccharides					701:715	fibre polysaccharides	695:715	fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%)	695:774	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	0	36	dep	Corylus	51:57	arg1	L.					68:69	Corylus avellana L.	51:69	Corylus avellana L.	51:69	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.					
32036182	5	37	contain	had	844:846	arg2	proportion					854:863	lower proportion	848:863	lower proportion of smooth region	848:880	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	5	37	contain	had	844:846	arg1	polysaccharides					820:834	pectic polysaccharides	813:834	pectic polysaccharides in IDFs	813:842	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	5	37	contain	had	844:846	arg2	proportion					893:902	higher proportion	886:902	higher proportion of hairy region that is heavily branched with arabinan and galactan side chains	886:982	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	1	38	theme	hazelnut	161:168	arg1	skin					170:173	hazelnut skin	161:173	hazelnut skin	161:173	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin were analyzed.					
32036182	0	39	theme	Tombul	34:39	arg1	Corylus					51:57	Corylus	51:57	Corylus	51:57	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.					
32036182	0	39	theme	Tombul	34:39	arg1	hazelnut					41:48	Turkish Tombul hazelnut	26:48	Turkish Tombul hazelnut (Corylus avellana L.)	26:70	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.					
32036182	4	40	theme	pectic	584:589	arg1	polysaccharides					591:605	pectic polysaccharides	584:605	pectic polysaccharides (~30%)	584:612	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	4	40	theme	pectic	584:589	arg1	%					611:611	~30%	608:611	~30%	608:611	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	5	41	theme	smooth	868:873	arg1	region					875:880	smooth region	868:880	smooth region	868:880	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	4	42	theme	hazelnut	655:662	arg1	skin					664:667	hazelnut skin	655:667	hazelnut skin	655:667	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	1	43	theme	skin	170:173	arg1	profiles					110:117	Dietary fibre (DF) profiles	91:117	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin	91:173	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin were analyzed.					
32036182	0	44	theme	Turkish	26:32	arg1	Corylus					51:57	Corylus	51:57	Corylus	51:57	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.					
32036182	0	44	theme	Turkish	26:32	arg1	hazelnut					41:48	Turkish Tombul hazelnut	26:48	Turkish Tombul hazelnut (Corylus avellana L.)	26:70	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.					
32036182	8	45	theme	large	1330:1334	arg1	function					1342:1349	large bowel function	1330:1349	large bowel function	1330:1349	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	3	46	theme	natural	368:374	arg1	hazelnut					376:383	natural hazelnut	368:383	natural hazelnut	368:383	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin were 17.8, 15.4, and 69.8%, respectively; majority of which (>96%) were water-insoluble.					
32036182	5	47	theme	region	875:880	arg1	proportion					854:863	lower proportion	848:863	lower proportion of smooth region	848:880	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	5	47	theme	region	875:880	arg1	proportion					893:902	higher proportion	886:902	higher proportion of hairy region that is heavily branched with arabinan and galactan side chains	886:982	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	0	48	dep	hazelnut	41:48	arg1	skin					85:88	skin	85:88	skin	85:88	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.					
32036182	2	49	dep	Insoluble	190:198	arg1	IDF					201:203	IDF	201:203	IDF	201:203	Insoluble (IDF) and soluble (SDF) DFs were examined for monosaccharide and glycosyl-linkage compositions using gas chromatography-mass spectrometry (GC-MS).					
32036182	3	50	theme	DF	353:354	arg1	contents					356:363	Total DF contents	347:363	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin	347:420	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin were 17.8, 15.4, and 69.8%, respectively; majority of which (>96%) were water-insoluble.					
32036182	4	51	theme	fibre	695:699	arg1	cellulose					718:726	cellulose	718:726	cellulose	718:726	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	4	51	theme	fibre	695:699	arg1	polysaccharides					736:750	pectic polysaccharides	729:750	pectic polysaccharides	729:750	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	4	51	theme	fibre	695:699	arg1	polysaccharides					701:715	fibre polysaccharides	695:715	fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%)	695:774	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	4	51	theme	fibre	695:699	arg1	xyloglucans					757:767	xyloglucans	757:767	xyloglucans	757:767	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	3	52	theme	roasted	386:392	arg1	hazelnut					394:401	roasted hazelnut	386:401	roasted hazelnut	386:401	Total DF contents of natural hazelnut, roasted hazelnut, and hazelnut skin were 17.8, 15.4, and 69.8%, respectively; majority of which (>96%) were water-insoluble.					
32036182	7	53	theme	branched	1152:1159	arg1	polysaccharides					1168:1182	slightly branched pectic polysaccharides	1143:1182	slightly branched pectic polysaccharides (~25%)	1143:1189	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	7	53	theme	branched	1152:1159	arg1	%					1188:1188	~25%	1185:1188	~25%	1185:1188	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	0	54	theme	hazelnut	41:48	arg1	profiles					14:21	Dietary fibre profiles	0:21	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.	0:89	Dietary fibre profiles of Turkish Tombul hazelnut (Corylus avellana L.) and hazelnut skin.					
32036182	6	55	theme	monomeric	1029:1037	arg1	chains					1059:1064	monomeric and/or dimeric side chains	1029:1064	monomeric and/or dimeric side chains	1029:1064	Xyloglucans were also densely branched with monomeric and/or dimeric side chains.					
32036182	7	56	theme	pectic	1161:1166	arg1	polysaccharides					1168:1182	slightly branched pectic polysaccharides	1143:1182	slightly branched pectic polysaccharides (~25%)	1143:1189	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	7	56	theme	pectic	1161:1166	arg1	%					1188:1188	~25%	1185:1188	~25%	1185:1188	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	1	57	theme	fibre	99:103	arg1	profiles					110:117	Dietary fibre (DF) profiles	91:117	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin	91:173	Dietary fibre (DF) profiles of natural hazelnut, roasted hazelnut and hazelnut skin were analyzed.					
32036182	8	58	theme	process	1403:1409	arg1	skin					1364:1367	hazelnut skin	1355:1367	hazelnut skin	1355:1367	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	8	58	theme	process	1403:1409	arg1	byproduct					1372:1380	a byproduct	1370:1380	a byproduct of hazelnut roasting process	1370:1409	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	7	59	contain	possessing	1208:1217	arg1	xyloglucans					1196:1206	xyloglucans	1196:1206	xyloglucans possessing monomeric side chains (~5%)	1196:1245	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	7	59	contain	possessing	1208:1217	arg1	%					1244:1244	~5%	1242:1244	~5%	1242:1244	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	7	59	contain	possessing	1208:1217	arg2	chains					1234:1239	monomeric side chains	1219:1239	monomeric side chains	1219:1239	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	5	60	theme	pectic	813:818	arg1	polysaccharides					820:834	pectic polysaccharides	813:834	pectic polysaccharides in IDFs	813:842	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	2	61	theme	soluble	210:216	arg1	DFs					224:226	Insoluble (IDF) and soluble (SDF) DFs	190:226	Insoluble (IDF) and soluble (SDF) DFs	190:226	Insoluble (IDF) and soluble (SDF) DFs were examined for monosaccharide and glycosyl-linkage compositions using gas chromatography-mass spectrometry (GC-MS).					
32036182	2	62	theme	Insoluble	190:198	arg1	DFs					224:226	Insoluble (IDF) and soluble (SDF) DFs	190:226	Insoluble (IDF) and soluble (SDF) DFs	190:226	Insoluble (IDF) and soluble (SDF) DFs were examined for monosaccharide and glycosyl-linkage compositions using gas chromatography-mass spectrometry (GC-MS).					
32036182	2	63	theme	glycosyl-linkage	265:280	arg1	compositions					282:293	monosaccharide and glycosyl-linkage compositions	246:293	compositions	282:293	Insoluble (IDF) and soluble (SDF) DFs were examined for monosaccharide and glycosyl-linkage compositions using gas chromatography-mass spectrometry (GC-MS).					
32036182	8	64	theme	roasting	1394:1401	arg1	process					1403:1409	hazelnut roasting process	1385:1409	hazelnut roasting process	1385:1409	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	8	65	theme	hazelnut	1355:1362	arg1	skin					1364:1367	hazelnut skin	1355:1367	hazelnut skin	1355:1367	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	8	65	theme	hazelnut	1355:1362	arg1	byproduct					1372:1380	a byproduct	1370:1380	a byproduct of hazelnut roasting process	1370:1409	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	5	66	theme	lower	848:852	arg1	proportion					854:863	lower proportion	848:863	lower proportion of smooth region	848:880	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	7	67	theme	branched	1112:1119	arg1	heteromannans					1121:1133	heavily branched heteromannans	1104:1133	heavily branched heteromannans (~60%)	1104:1140	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	7	67	theme	branched	1112:1119	arg1	%					1139:1139	~60%	1136:1139	~60%	1136:1139	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	4	68	theme	hazelnuts	539:547	arg1	IDFs					511:514	IDFs	511:514	IDFs of natural and roasted hazelnuts	511:547	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	5	69	theme	other	798:802	arg1	sources					804:810	other sources	798:810	other sources	798:810	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	4	70	theme	roasted	531:537	arg1	hazelnuts					539:547	natural and roasted hazelnuts	519:547	natural and roasted hazelnuts	519:547	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	8	71	theme	prebiotic	1484:1492	arg1	capacities					1494:1503	prebiotic capacities	1484:1503	prebiotic capacities	1484:1503	These results suggest that hazelnut is rich in DFs that have potential to improve large bowel function and hazelnut skin, a byproduct of hazelnut roasting process, could be utilized for the production of functional carbohydrates having prebiotic capacities.					
32036182	4	72	theme	natural	519:525	arg1	hazelnuts					539:547	natural and roasted hazelnuts	519:547	natural and roasted hazelnuts	519:547	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	4	73	dep	cellulose	718:726	arg1	%					773:773	~45%	770:773	~45%	770:773	IDFs of natural and roasted hazelnuts were composed of cellulose (~49%), pectic polysaccharides (~30%), and xyloglucans (~15%), whereas that of hazelnut skin made up lignin (~55%) and fibre polysaccharides (cellulose, pectic polysaccharides, and xyloglucans, ~45%).					
32036182	5	74	theme	region	913:918	arg1	proportion					854:863	lower proportion	848:863	lower proportion of smooth region	848:880	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	5	74	theme	region	913:918	arg1	proportion					893:902	higher proportion	886:902	higher proportion of hairy region that is heavily branched with arabinan and galactan side chains	886:982	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
32036182	7	75	theme	samples	1079:1085	arg1	SDFs					1067:1070	SDFs	1067:1070	SDFs of the samples	1067:1085	SDFs of the samples were composed of heavily branched heteromannans (~60%), slightly branched pectic polysaccharides (~25%), and xyloglucans possessing monomeric side chains (~5%).					
32036182	5	76	from	sources	804:810	arg1	ones					788:791	the ones	784:791	the ones from other sources	784:810	Unlike the ones from other sources, pectic polysaccharides in IDFs had lower proportion of smooth region and higher proportion of hairy region that is heavily branched with arabinan and galactan side chains.					
34113353	0	0	theme	Downstream	71:80	arg1	Mechanism					92:100	the Downstream Signaling Mechanism	67:100	the Downstream Signaling Mechanism	67:100	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.					
34113353	1	1	attach	attached	111:118	arg2	Glycans					103:109	Glycans	103:109	Glycans attached to immunoglobulin G (IgG)	103:144	Glycans attached to immunoglobulin G (IgG) directly affect this antibody effector functions and regulate inflammation at several levels.					
34113353	1	1	attach	attached	111:118	arg1	IgG					141:143	IgG	141:143	IgG	141:143	Glycans attached to immunoglobulin G (IgG) directly affect this antibody effector functions and regulate inflammation at several levels.					
34113353	1	1	attach	attached	111:118	arg1	G					138:138	immunoglobulin G	123:138	immunoglobulin G (IgG)	123:144	Glycans attached to immunoglobulin G (IgG) directly affect this antibody effector functions and regulate inflammation at several levels.					
34113353	5	2	from	changes	813:819	arg1	glycome					832:838	the IgG glycome	824:838	the IgG glycome	824:838	The suppression of gonadal hormones induced significant changes in the IgG glycome, while E2 supplementation was sufficient to prevent changes.					
34113353	6	3	theme	glycan	914:919	arg1	changes					921:927	The observed glycan changes	901:927	The observed glycan changes	901:927	The observed glycan changes suggest that depletion of E2 primarily affects B cell glycosylation, while liver glycosylation stays mostly unchanged.					
34113353	4	4	theme	healthy	493:499	arg1	women					515:519	36 healthy premenopausal women	490:519	36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch	490:754	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	8	5	theme	RUNX3	1491:1495	arg1	case					1483:1486	the case	1479:1486	the case of RUNX3	1479:1495	RUNX3 and SPINK4 upregulation using dCas9-VPR resulted in a decreased IgG galactosylation and, in the case of RUNX3, a concomitant increase in IgG agalactosylation.					
34113353	8	6	theme	concomitant	1500:1510	arg1	increase					1512:1519	a concomitant increase	1498:1519	a concomitant increase in IgG agalactosylation	1498:1543	RUNX3 and SPINK4 upregulation using dCas9-VPR resulted in a decreased IgG galactosylation and, in the case of RUNX3, a concomitant increase in IgG agalactosylation.					
34113353	0	7	theme	Mechanism	92:100	arg1	Mapping					56:62	Mapping	56:62	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.	0:101	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.					
34113353	0	8	from	Effects	0:6	arg1	Glycosylation					41:53	Immunoglobulin G Glycosylation	24:53	Immunoglobulin G Glycosylation	24:53	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.					
34113353	4	9	from	effect	405:410	arg1	glycosylation					431:443	IgG glycosylation	427:443	IgG glycosylation	427:443	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	4	10	theme	hormone	593:599	arg1	acetate					630:636	the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate	566:636	the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate	566:636	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	0	11	theme	Signaling	82:90	arg1	Mechanism					92:100	the Downstream Signaling Mechanism	67:100	the Downstream Signaling Mechanism	67:100	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.					
34113353	8	12	theme	IgG	1524:1526	arg1	agalactosylation					1528:1543	IgG agalactosylation	1524:1543	IgG agalactosylation	1524:1543	RUNX3 and SPINK4 upregulation using dCas9-VPR resulted in a decreased IgG galactosylation and, in the case of RUNX3, a concomitant increase in IgG agalactosylation.					
34113353	4	13	theme	gonadotropin-releasing	570:591	arg1	acetate					630:636	the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate	566:636	the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate	566:636	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	7	14	theme	downstream	1152:1161	arg1	mechanisms					1173:1182	downstream signaling mechanisms	1152:1182	downstream signaling mechanisms	1152:1182	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	15	theme	integrated	1303:1312	arg1	cassettes					1338:1346	stably integrated CRISPR/dCas9 expression cassettes	1296:1346	stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation	1296:1378	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	4	16	theme	postmenopausal	667:680	arg1	levels					682:687	postmenopausal levels	667:687	postmenopausal levels	667:687	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	5	17	theme	E2	847:848	arg1	supplementation					850:864	E2 supplementation	847:864	E2 supplementation	847:864	The suppression of gonadal hormones induced significant changes in the IgG glycome, while E2 supplementation was sufficient to prevent changes.					
34113353	6	18	theme	cell	978:981	arg1	glycosylation					983:995	B cell glycosylation	976:995	B cell glycosylation	976:995	The observed glycan changes suggest that depletion of E2 primarily affects B cell glycosylation, while liver glycosylation stays mostly unchanged.					
34113353	4	19	theme	serum	472:476	arg1	glycomes					478:485	total serum glycomes	466:485	total serum glycomes	466:485	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	2	20	theme	glycome	263:269	arg1	composition					244:254	The composition	240:254	The composition of IgG glycome	240:269	The composition of IgG glycome changes significantly with age.					
34113353	4	21	theme	gonadal	647:653	arg1	steroids					655:662	lower gonadal steroids	641:662	lower gonadal steroids	641:662	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	5	22	theme	significant	801:811	arg1	changes					813:819	significant changes	801:819	significant changes in the IgG glycome	801:838	The suppression of gonadal hormones induced significant changes in the IgG glycome, while E2 supplementation was sufficient to prevent changes.					
34113353	4	23	theme	total	466:470	arg1	glycomes					478:485	total serum glycomes	466:485	total serum glycomes	466:485	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	7	24	theme	GWAS	1095:1098	arg1	RUNX1					1105:1109	RUNX1	1105:1109	RUNX1	1105:1109	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	24	theme	GWAS	1095:1098	arg1	SPINK4					1119:1124	SPINK4	1119:1124	SPINK4	1119:1124	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	24	theme	GWAS	1095:1098	arg1	hits					1100:1103	previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2	1069:1134	previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2	1069:1134	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	24	theme	GWAS	1095:1098	arg1	RUNX3					1112:1116	RUNX3	1112:1116	RUNX3	1112:1116	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	24	theme	GWAS	1095:1098	arg1	ELL2					1131:1134	ELL2	1131:1134	ELL2	1131:1134	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	6	25	theme	B	976:976	arg1	glycosylation					983:995	B cell glycosylation	976:995	B cell glycosylation	976:995	The observed glycan changes suggest that depletion of E2 primarily affects B cell glycosylation, while liver glycosylation stays mostly unchanged.					
34113353	6	26	theme	observed	905:912	arg1	changes					921:927	The observed glycan changes	901:927	The observed glycan changes	901:927	The observed glycan changes suggest that depletion of E2 primarily affects B cell glycosylation, while liver glycosylation stays mostly unchanged.					
34113353	7	27	theme	signaling	1163:1171	arg1	mechanisms					1173:1182	downstream signaling mechanisms	1152:1182	downstream signaling mechanisms	1152:1182	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	1	28	theme	antibody	167:174	arg1	functions					185:193	this antibody effector functions	162:193	this antibody effector functions	162:193	Glycans attached to immunoglobulin G (IgG) directly affect this antibody effector functions and regulate inflammation at several levels.					
34113353	4	29	dep	women	515:519	arg1	enrolled					521:528	enrolled	521:528	enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels	521:687	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	4	29	dep	women	515:519	arg1	randomized					698:707	randomized	698:707	then randomized to transdermal placebo or estradiol (E2) patch	693:754	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	4	30	theme	randomized	535:544	arg1	trial					557:561	a randomized controlled trial	533:561	a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids	533:662	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	4	31	dep	placebo	724:730	arg1	patch					750:754	patch	750:754	patch	750:754	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	1	32	theme	effector	176:183	arg1	functions					185:193	this antibody effector functions	162:193	this antibody effector functions	162:193	Glycans attached to immunoglobulin G (IgG) directly affect this antibody effector functions and regulate inflammation at several levels.					
34113353	3	33	theme	perimenopausal	356:369	arg1	period					371:376	the perimenopausal period	352:376	the perimenopausal period	352:376	In women, the most notable change coincides with the perimenopausal period.					
34113353	7	34	theme	identified	1080:1089	arg1	RUNX1					1105:1109	RUNX1	1105:1109	RUNX1	1105:1109	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	34	theme	identified	1080:1089	arg1	SPINK4					1119:1124	SPINK4	1119:1124	SPINK4	1119:1124	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	34	theme	identified	1080:1089	arg1	hits					1100:1103	previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2	1069:1134	previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2	1069:1134	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	34	theme	identified	1080:1089	arg1	RUNX3					1112:1116	RUNX3	1112:1116	RUNX3	1112:1116	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	34	theme	identified	1080:1089	arg1	ELL2					1131:1134	ELL2	1131:1134	ELL2	1131:1134	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	8	35	theme	SPINK4	1391:1396	arg1	upregulation					1398:1409	SPINK4 upregulation	1391:1409	SPINK4 upregulation	1391:1409	RUNX3 and SPINK4 upregulation using dCas9-VPR resulted in a decreased IgG galactosylation and, in the case of RUNX3, a concomitant increase in IgG agalactosylation.					
34113353	4	36	theme	acetate	630:636	arg1	trial					557:561	a randomized controlled trial	533:561	a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids	533:662	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	0	37	theme	Estradiol	11:19	arg1	Effects					0:6	Effects	0:6	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.	0:101	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.					
34113353	7	38	theme	IgG	1091:1093	arg1	RUNX1					1105:1109	RUNX1	1105:1109	RUNX1	1105:1109	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	38	theme	IgG	1091:1093	arg1	SPINK4					1119:1124	SPINK4	1119:1124	SPINK4	1119:1124	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	38	theme	IgG	1091:1093	arg1	hits					1100:1103	previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2	1069:1134	previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2	1069:1134	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	38	theme	IgG	1091:1093	arg1	RUNX3					1112:1116	RUNX3	1112:1116	RUNX3	1112:1116	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	38	theme	IgG	1091:1093	arg1	ELL2					1131:1134	ELL2	1131:1134	ELL2	1131:1134	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	39	theme	CRISPR/dCas9	1314:1325	arg1	cassettes					1338:1346	stably integrated CRISPR/dCas9 expression cassettes	1296:1346	stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation	1296:1378	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	4	40	theme	leuprolide	619:628	arg1	acetate					630:636	the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate	566:636	the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate	566:636	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	0	41	theme	G	39:39	arg1	Glycosylation					41:53	Immunoglobulin G Glycosylation	24:53	Immunoglobulin G Glycosylation	24:53	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.					
34113353	4	42	theme	controlled	546:555	arg1	trial					557:561	a randomized controlled trial	533:561	a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids	533:662	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	6	43	theme	E2	955:956	arg1	depletion					942:950	depletion	942:950	depletion of E2	942:956	The observed glycan changes suggest that depletion of E2 primarily affects B cell glycosylation, while liver glycosylation stays mostly unchanged.					
34113353	7	44	theme	expression	1327:1336	arg1	cassettes					1338:1346	stably integrated CRISPR/dCas9 expression cassettes	1296:1346	stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation	1296:1378	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	4	45	theme	lower	641:645	arg1	steroids					655:662	lower gonadal steroids	641:662	lower gonadal steroids	641:662	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	0	46	theme	Immunoglobulin	24:37	arg1	Glycosylation					41:53	Immunoglobulin G Glycosylation	24:53	Immunoglobulin G Glycosylation	24:53	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.					
34113353	8	47	from	increase	1512:1519	arg1	agalactosylation					1528:1543	IgG agalactosylation	1524:1543	IgG agalactosylation	1524:1543	RUNX3 and SPINK4 upregulation using dCas9-VPR resulted in a decreased IgG galactosylation and, in the case of RUNX3, a concomitant increase in IgG agalactosylation.					
34113353	5	48	theme	gonadal	776:782	arg1	hormones					784:791	gonadal hormones	776:791	gonadal hormones	776:791	The suppression of gonadal hormones induced significant changes in the IgG glycome, while E2 supplementation was sufficient to prevent changes.					
34113353	6	49	theme	liver	1004:1008	arg1	glycosylation					1010:1022	liver glycosylation	1004:1022	liver glycosylation	1004:1022	The observed glycan changes suggest that depletion of E2 primarily affects B cell glycosylation, while liver glycosylation stays mostly unchanged.					
34113353	7	50	dep	gene	1352:1355	arg1	downregulation					1365:1378	downregulation	1365:1378	downregulation	1365:1378	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	50	dep	gene	1352:1355	arg1	up-					1357:1359	up-	1357:1359	up-	1357:1359	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	5	51	theme	hormones	784:791	arg1	suppression					761:771	The suppression	757:771	The suppression of gonadal hormones	757:791	The suppression of gonadal hormones induced significant changes in the IgG glycome, while E2 supplementation was sufficient to prevent changes.					
34113353	4	52	theme	premenopausal	501:513	arg1	women					515:519	36 healthy premenopausal women	490:519	36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch	490:754	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	7	53	dep	hits	1100:1103	arg1	RUNX1					1105:1109	RUNX1	1105:1109	RUNX1	1105:1109	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	53	dep	hits	1100:1103	arg1	SPINK4					1119:1124	SPINK4	1119:1124	SPINK4	1119:1124	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	53	dep	hits	1100:1103	arg1	ELL2					1131:1134	ELL2	1131:1134	ELL2	1131:1134	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	53	dep	hits	1100:1103	arg1	RUNX3					1112:1116	RUNX3	1112:1116	RUNX3	1112:1116	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	53	dep	hits	1100:1103	arg1	hits					1100:1103	previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2	1069:1134	previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2	1069:1134	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	4	54	theme	transdermal	712:722	arg1	placebo					724:730	transdermal placebo	712:730	transdermal placebo	712:730	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	4	55	theme	analogue	601:608	arg1	acetate					630:636	the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate	566:636	the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate	566:636	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	4	56	theme	estrogen	415:422	arg1	effect					405:410	the effect	401:410	the effect of estrogen on IgG glycosylation	401:443	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	7	57	theme	IgG	1276:1278	arg1	antibodies					1280:1289	IgG antibodies	1276:1289	IgG antibodies	1276:1289	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	8	58	theme	decreased	1441:1449	arg1	galactosylation					1455:1469	a decreased IgG galactosylation	1439:1469	a decreased IgG galactosylation	1439:1469	RUNX3 and SPINK4 upregulation using dCas9-VPR resulted in a decreased IgG galactosylation and, in the case of RUNX3, a concomitant increase in IgG agalactosylation.					
34113353	2	59	theme	IgG	259:261	arg1	glycome					263:269	IgG glycome	259:269	IgG glycome	259:269	The composition of IgG glycome changes significantly with age.					
34113353	1	60	theme	immunoglobulin	123:136	arg1	IgG					141:143	IgG	141:143	IgG	141:143	Glycans attached to immunoglobulin G (IgG) directly affect this antibody effector functions and regulate inflammation at several levels.					
34113353	1	60	theme	immunoglobulin	123:136	arg1	G					138:138	immunoglobulin G	123:138	immunoglobulin G (IgG)	123:144	Glycans attached to immunoglobulin G (IgG) directly affect this antibody effector functions and regulate inflammation at several levels.					
34113353	7	61	theme	IgG	1201:1203	arg1	glycosylation					1205:1217	IgG glycosylation	1201:1217	IgG glycosylation	1201:1217	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	4	62	theme	IgG	427:429	arg1	glycosylation					431:443	IgG glycosylation	427:443	IgG glycosylation	427:443	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
34113353	7	63	used	used	1223:1226	arg2	we					1220:1221	we	1220:1221	we	1220:1221	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	1	64	theme	several	224:230	arg1	levels					232:237	several levels	224:237	several levels	224:237	Glycans attached to immunoglobulin G (IgG) directly affect this antibody effector functions and regulate inflammation at several levels.					
34113353	8	65	theme	IgG	1451:1453	arg1	galactosylation					1455:1469	a decreased IgG galactosylation	1439:1469	a decreased IgG galactosylation	1439:1469	RUNX3 and SPINK4 upregulation using dCas9-VPR resulted in a decreased IgG galactosylation and, in the case of RUNX3, a concomitant increase in IgG agalactosylation.					
34113353	5	66	theme	IgG	828:830	arg1	glycome					832:838	the IgG glycome	824:838	the IgG glycome	824:838	The suppression of gonadal hormones induced significant changes in the IgG glycome, while E2 supplementation was sufficient to prevent changes.					
34113353	7	67	theme	FreeStyle	1232:1240	arg1	system					1258:1263	the FreeStyle 293-F transient system	1228:1263	the FreeStyle 293-F transient system	1228:1263	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	3	68	theme	notable	322:328	arg1	change					330:335	the most notable change	313:335	the most notable change	313:335	In women, the most notable change coincides with the perimenopausal period.					
34113353	0	69	dep	Effects	0:6	arg1	Mapping					56:62	Mapping	56:62	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.	0:101	Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism.					
34113353	7	70	theme	transient	1248:1256	arg1	system					1258:1263	the FreeStyle 293-F transient system	1228:1263	the FreeStyle 293-F transient system	1228:1263	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	7	71	theme	293-F	1242:1246	arg1	system					1258:1263	the FreeStyle 293-F transient system	1228:1263	the FreeStyle 293-F transient system	1228:1263	To determine whether previously identified IgG GWAS hits RUNX1, RUNX3, SPINK4, and ELL2 are involved in downstream signaling mechanisms, linking E2 with IgG glycosylation, we used the FreeStyle 293-F transient system expressing IgG antibodies with stably integrated CRISPR/dCas9 expression cassettes for gene up- and downregulation.					
34113353	4	72	theme	GnRHAG	611:616	arg1	acetate					630:636	the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate	566:636	the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate	566:636	Aiming to investigate the effect of estrogen on IgG glycosylation, we analysed IgG and total serum glycomes in 36 healthy premenopausal women enrolled in a randomized controlled trial of the gonadotropin-releasing hormone analogue (GnRHAG) leuprolide acetate to lower gonadal steroids to postmenopausal levels and then randomized to transdermal placebo or estradiol (E2) patch.					
32075084	0	0	theme	CMS	86:88	arg1	Composites					98:107	Alkali-Activated Slag /Starch(CMS) Hybrid Composites	56:107	Alkali-Activated Slag /Starch(CMS) Hybrid Composites	56:107	One-Part Plastic Formable Inorganic Coating Obtain from Alkali-Activated Slag /Starch(CMS) Hybrid Composites.					
32075084	7	1	theme	water	1450:1454	arg1	retention					1456:1464	water retention	1450:1464	water retention	1450:1464	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	5	2	dep	strengths	816:824	arg1	flexural					840:847	flexural	840:847	flexural	840:847	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	5	2	dep	strengths	816:824	arg1	compressive					827:837	compressive	827:837	compressive	827:837	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	5	2	dep	strengths	816:824	arg1	strength					863:870	adhesive strength	854:870	adhesive strength	854:870	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	7	3	from	effect	1379:1384	arg1	the					1389:1391	the	1389:1391	the	1389:1391	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	3	from	effect	1379:1384	arg1	control					1416:1422	shrinkage control	1406:1422	shrinkage control	1406:1422	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	6	4	theme	AAM	1075:1077	arg1	paste					1079:1083	AAM paste	1075:1083	AAM paste	1075:1083	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	7	5	theme	padding	1431:1437	arg1	effect					1439:1444	padding effect	1431:1444	padding effect	1431:1444	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	1	6	theme	building	156:163	arg1	constructions					165:177	building constructions	156:177	building constructions	156:177	Coating technology can be applied to decorate building constructions.					
32075084	0	7	theme	Hybrid	91:96	arg1	Composites					98:107	Alkali-Activated Slag /Starch(CMS) Hybrid Composites	56:107	Alkali-Activated Slag /Starch(CMS) Hybrid Composites	56:107	One-Part Plastic Formable Inorganic Coating Obtain from Alkali-Activated Slag /Starch(CMS) Hybrid Composites.					
32075084	7	8	theme	sufficient	1302:1311	arg1	time					1297:1300	the setting time	1285:1300	the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface	1285:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	8	theme	sufficient	1302:1311	arg1	development					1272:1282	a relatively stable rheological development	1240:1282	a relatively stable rheological development	1240:1282	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	5	9	theme	different	655:663	arg1	mixtures					665:672	Four different mixtures	650:672	Four different mixtures	650:672	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	7	10	theme	optimum	1188:1194	arg1	coating					1196:1202	The optimum coating	1184:1202	The optimum coating composited with 15.40 wt% CMS	1184:1232	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	11	theme	significant	1358:1368	arg1	effect					1379:1384	a significant positive effect	1356:1384	a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface	1356:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	12	theme	visible	1494:1500	arg1	cracks					1502:1507	no visible cracks	1491:1507	no visible cracks on the coating surface	1491:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	13	theme	positive	1370:1377	arg1	effect					1379:1384	a significant positive effect	1356:1384	a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface	1356:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	14	theme	due	1424:1426	arg1	effect					1379:1384	a significant positive effect	1356:1384	a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface	1356:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	15	theme	wt	1226:1227	arg1	CMS					1230:1232	15.40 wt% CMS	1220:1232	15.40 wt% CMS	1220:1232	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	6	16	theme	viscosity	1157:1165	arg1	improvement					1113:1123	the significant improvement	1097:1123	the significant improvement of coating consistency, plastic viscosity, and thixotropy	1097:1181	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	7	17	from	higher	1316:1321	arg1	sufficient					1302:1311	sufficient	1302:1311	sufficient	1302:1311	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	5	18	theme	cracking	892:899	arg1	behavior					901:908	cracking behavior	892:908	cracking behavior	892:908	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	5	18	theme	cracking	892:899	arg1	strengths					816:824	mechanical strengths	805:824	mechanical strengths (compressive, flexural, and adhesive strength)	805:871	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	5	18	theme	cracking	892:899	arg1	properties					785:794	their hardened properties	770:794	their hardened properties	770:794	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	6	19	theme	plastic	1149:1155	arg1	viscosity					1157:1165	plastic viscosity	1149:1165	plastic viscosity	1149:1165	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	7	20	theme	setting	1289:1295	arg1	time					1297:1300	the setting time	1285:1300	the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface	1285:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	20	theme	setting	1289:1295	arg1	development					1272:1282	a relatively stable rheological development	1240:1282	a relatively stable rheological development	1240:1282	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	3	21	theme	formable	414:421	arg1	coating					433:439	a novel plastic formable inorganic coating	398:439	a novel plastic formable inorganic coating for decorating in building	398:466	In the paper, the possibility of using AAM composited with starch (CMS) as a novel plastic formable inorganic coating for decorating in building was investigated.					
32075084	3	21	theme	formable	414:421	arg1	AAM					362:364	AAM	362:364	AAM composited with starch (CMS)	362:393	In the paper, the possibility of using AAM composited with starch (CMS) as a novel plastic formable inorganic coating for decorating in building was investigated.					
32075084	7	22	theme	shrinkage	1406:1414	arg1	control					1416:1422	shrinkage control	1406:1422	shrinkage control	1406:1422	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	5	23	theme	mechanical	805:814	arg1	strengths					816:824	mechanical strengths	805:824	mechanical strengths (compressive, flexural, and adhesive strength)	805:871	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	3	24	theme	inorganic	423:431	arg1	coating					433:439	a novel plastic formable inorganic coating	398:439	a novel plastic formable inorganic coating for decorating in building	398:466	In the paper, the possibility of using AAM composited with starch (CMS) as a novel plastic formable inorganic coating for decorating in building was investigated.					
32075084	3	24	theme	inorganic	423:431	arg1	AAM					362:364	AAM	362:364	AAM composited with starch (CMS)	362:393	In the paper, the possibility of using AAM composited with starch (CMS) as a novel plastic formable inorganic coating for decorating in building was investigated.					
32075084	0	25	theme	Plastic	9:15	arg1	Coating					36:42	One-Part Plastic Formable Inorganic Coating	0:42	One-Part Plastic Formable Inorganic Coating	0:42	One-Part Plastic Formable Inorganic Coating Obtain from Alkali-Activated Slag /Starch(CMS) Hybrid Composites.					
32075084	6	26	theme	consistency	1136:1146	arg1	improvement					1113:1123	the significant improvement	1097:1123	the significant improvement of coating consistency, plastic viscosity, and thixotropy	1097:1181	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	3	27	theme	novel	400:404	arg1	coating					433:439	a novel plastic formable inorganic coating	398:439	a novel plastic formable inorganic coating for decorating in building	398:466	In the paper, the possibility of using AAM composited with starch (CMS) as a novel plastic formable inorganic coating for decorating in building was investigated.					
32075084	3	27	theme	novel	400:404	arg1	AAM					362:364	AAM	362:364	AAM composited with starch (CMS)	362:393	In the paper, the possibility of using AAM composited with starch (CMS) as a novel plastic formable inorganic coating for decorating in building was investigated.					
32075084	0	28	theme	One-Part	0:7	arg1	Coating					36:42	One-Part Plastic Formable Inorganic Coating	0:42	One-Part Plastic Formable Inorganic Coating	0:42	One-Part Plastic Formable Inorganic Coating Obtain from Alkali-Activated Slag /Starch(CMS) Hybrid Composites.					
32075084	5	29	used	used	756:759	arg2	mixtures					665:672	Four different mixtures	650:672	Four different mixtures	650:672	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	6	30	theme	coating	1128:1134	arg1	consistency					1136:1146	coating consistency	1128:1146	coating consistency	1128:1146	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	8	31	theme	compressive	1661:1671	arg1	MPa					1688:1690	compressive strength 55.09 MPa	1661:1690	compressive strength 55.09 MPa	1661:1690	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	8	31	theme	compressive	1661:1671	arg1	value					1626:1630	its value	1622:1630	its value	1622:1630	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	3	32	theme	plastic	406:412	arg1	coating					433:439	a novel plastic formable inorganic coating	398:439	a novel plastic formable inorganic coating for decorating in building	398:466	In the paper, the possibility of using AAM composited with starch (CMS) as a novel plastic formable inorganic coating for decorating in building was investigated.					
32075084	3	32	theme	plastic	406:412	arg1	AAM					362:364	AAM	362:364	AAM composited with starch (CMS)	362:393	In the paper, the possibility of using AAM composited with starch (CMS) as a novel plastic formable inorganic coating for decorating in building was investigated.					
32075084	0	33	theme	Inorganic	26:34	arg1	Coating					36:42	One-Part Plastic Formable Inorganic Coating	0:42	One-Part Plastic Formable Inorganic Coating	0:42	One-Part Plastic Formable Inorganic Coating Obtain from Alkali-Activated Slag /Starch(CMS) Hybrid Composites.					
32075084	4	34	theme	yield	543:547	arg1	stress					549:554	yield stress	543:554	yield stress	543:554	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	8	35	theme	standard	1767:1774	arg1	requirements					1740:1751	the requirements	1736:1751	the requirements of a relevant standard	1736:1774	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	6	36	theme	organic	1039:1045	arg1	gel					1055:1057	organic plastic gel	1039:1057	organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy	1039:1181	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	0	37	theme	Formable	17:24	arg1	Coating					36:42	One-Part Plastic Formable Inorganic Coating	0:42	One-Part Plastic Formable Inorganic Coating	0:42	One-Part Plastic Formable Inorganic Coating Obtain from Alkali-Activated Slag /Starch(CMS) Hybrid Composites.					
32075084	7	38	from	cracks	1502:1507	arg1	surface					1524:1530	the coating surface	1512:1530	the coating surface	1512:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	2	39	theme	innovative	303:312	arg1	coating					314:320	innovative coating	303:320	innovative coating	303:320	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	5	40	theme	hardened	776:783	arg1	shrinkage					881:889	drying shrinkage	874:889	drying shrinkage	874:889	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	5	40	theme	hardened	776:783	arg1	behavior					901:908	cracking behavior	892:908	cracking behavior	892:908	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	5	40	theme	hardened	776:783	arg1	microstructure					915:928	microstructure	915:928	microstructure	915:928	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	5	40	theme	hardened	776:783	arg1	strengths					816:824	mechanical strengths	805:824	mechanical strengths (compressive, flexural, and adhesive strength)	805:871	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	5	40	theme	hardened	776:783	arg1	properties					785:794	their hardened properties	770:794	their hardened properties	770:794	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	8	41	theme	adhesive	1633:1640	arg1	MPa					1656:1658	adhesive strength 2.11 MPa	1633:1658	adhesive strength 2.11 MPa	1633:1658	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	8	41	theme	adhesive	1633:1640	arg1	value					1626:1630	its value	1622:1630	its value	1622:1630	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	5	42	theme	optimum	721:727	arg1	formulation					729:739	the optimum formulation	717:739	the optimum formulation	717:739	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	2	43	theme	Alkali-activated	180:195	arg1	AAM					208:210	AAM	208:210	AAM	208:210	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	2	43	theme	Alkali-activated	180:195	arg1	materials					197:205	Alkali-activated materials	180:205	Alkali-activated materials (AAM)	180:211	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	2	43	theme	Alkali-activated	180:195	arg1	binders					255:261	promising green and durable inorganic binders	217:261	promising green and durable inorganic binders which show potential for development as innovative coating	217:320	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	2	44	theme	durable	237:243	arg1	binders					255:261	promising green and durable inorganic binders	217:261	promising green and durable inorganic binders which show potential for development as innovative coating	217:320	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	2	44	theme	durable	237:243	arg1	materials					197:205	Alkali-activated materials	180:205	Alkali-activated materials (AAM)	180:211	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	6	45	theme	plastic	1047:1053	arg1	gel					1055:1057	organic plastic gel	1039:1057	organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy	1039:1181	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	7	46	theme	rheological	1260:1270	arg1	time					1297:1300	the setting time	1285:1300	the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface	1285:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	46	theme	rheological	1260:1270	arg1	development					1272:1282	a relatively stable rheological development	1240:1282	a relatively stable rheological development	1240:1282	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	6	47	theme	alkaline	1010:1017	arg1	solution					1019:1026	an alkaline solution	1007:1026	an alkaline solution	1007:1026	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	4	48	theme	plastic	524:530	arg1	viscosity					532:540	plastic viscosity	524:540	plastic viscosity	524:540	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	4	49	theme	working	628:634	arg1	requirements					636:647	the working requirements	624:647	the working requirements	624:647	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	0	50	theme	Alkali-Activated	56:71	arg1	Composites					98:107	Alkali-Activated Slag /Starch(CMS) Hybrid Composites	56:107	Alkali-Activated Slag /Starch(CMS) Hybrid Composites	56:107	One-Part Plastic Formable Inorganic Coating Obtain from Alkali-Activated Slag /Starch(CMS) Hybrid Composites.					
32075084	6	51	theme	Study	931:935	arg1	results					937:943	Study results	931:943	Study results	931:943	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	8	52	theme	strength	1673:1680	arg1	MPa					1688:1690	compressive strength 55.09 MPa	1661:1690	compressive strength 55.09 MPa	1661:1690	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	8	52	theme	strength	1673:1680	arg1	value					1626:1630	its value	1622:1630	its value	1622:1630	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	2	53	theme	green	227:231	arg1	binders					255:261	promising green and durable inorganic binders	217:261	promising green and durable inorganic binders which show potential for development as innovative coating	217:320	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	2	53	theme	green	227:231	arg1	materials					197:205	Alkali-activated materials	180:205	Alkali-activated materials (AAM)	180:211	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	1	54	theme	Coating	110:116	arg1	technology					118:127	Coating technology	110:127	Coating technology	110:127	Coating technology can be applied to decorate building constructions.					
32075084	8	55	theme	strength	1642:1649	arg1	MPa					1656:1658	adhesive strength 2.11 MPa	1633:1658	adhesive strength 2.11 MPa	1633:1658	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	8	55	theme	strength	1642:1649	arg1	value					1626:1630	its value	1622:1630	its value	1622:1630	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	7	56	theme	CMS	1469:1471	arg1	effect					1439:1444	padding effect	1431:1444	padding effect	1431:1444	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	56	theme	CMS	1469:1471	arg1	retention					1456:1464	water retention	1450:1464	water retention	1450:1464	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	2	57	theme	promising	217:225	arg1	binders					255:261	promising green and durable inorganic binders	217:261	promising green and durable inorganic binders which show potential for development as innovative coating	217:320	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	2	57	theme	promising	217:225	arg1	materials					197:205	Alkali-activated materials	180:205	Alkali-activated materials (AAM)	180:211	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	8	58	theme	flexural	1697:1704	arg1	value					1626:1630	its value	1622:1630	its value	1622:1630	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	8	58	theme	flexural	1697:1704	arg1	MPa					1720:1722	flexural strength 8.06 MPa	1697:1722	flexural strength 8.06 MPa	1697:1722	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	7	59	theme	stable	1253:1258	arg1	time					1297:1300	the setting time	1285:1300	the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface	1285:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	7	59	theme	stable	1253:1258	arg1	development					1272:1282	a relatively stable rheological development	1240:1282	a relatively stable rheological development	1240:1282	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	4	60	theme	critical	594:601	arg1	viscosity					532:540	plastic viscosity	524:540	plastic viscosity	524:540	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	4	60	theme	critical	594:601	arg1	properties					502:511	The rheological properties	486:511	The rheological properties	486:511	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	4	60	theme	critical	594:601	arg1	thixotropy					561:570	thixotropy	561:570	thixotropy	561:570	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	4	60	theme	critical	594:601	arg1	properties					603:612	critical properties	594:612	critical properties	594:612	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	4	60	theme	critical	594:601	arg1	stress					549:554	yield stress	543:554	yield stress	543:554	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	8	61	theme	plain	1611:1615	arg1	AAM					1617:1619	plain AAM	1611:1619	plain AAM	1611:1619	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	6	62	theme	significant	1101:1111	arg1	improvement					1113:1123	the significant improvement	1097:1123	the significant improvement of coating consistency, plastic viscosity, and thixotropy	1097:1181	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	8	63	theme	mechanical	1546:1555	arg1	development					1566:1576	the mechanical strength development	1542:1576	the mechanical strength development	1542:1576	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	8	63	theme	mechanical	1546:1555	arg1	lower					1592:1596	lower	1592:1596	lower	1592:1596	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	0	64	theme	/Starch	78:84	arg1	Composites					98:107	Alkali-Activated Slag /Starch(CMS) Hybrid Composites	56:107	Alkali-Activated Slag /Starch(CMS) Hybrid Composites	56:107	One-Part Plastic Formable Inorganic Coating Obtain from Alkali-Activated Slag /Starch(CMS) Hybrid Composites.					
32075084	7	65	dep	h.	1330:1331	arg1	shows					1350:1354	shows	1350:1354	shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface	1350:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	6	66	theme	thixotropy	1172:1181	arg1	improvement					1113:1123	the significant improvement	1097:1123	the significant improvement of coating consistency, plastic viscosity, and thixotropy	1097:1181	Study results found that CMS could quickly and efficiently be hydrolyzed in an alkaline solution to produce organic plastic gel which filled in AAM paste, leading to the significant improvement of coating consistency, plastic viscosity, and thixotropy.					
32075084	8	67	theme	strength	1706:1713	arg1	value					1626:1630	its value	1622:1630	its value	1622:1630	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	8	67	theme	strength	1706:1713	arg1	MPa					1720:1722	flexural strength 8.06 MPa	1697:1722	flexural strength 8.06 MPa	1697:1722	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	7	68	theme	%	1228:1228	arg1	CMS					1230:1232	15.40 wt% CMS	1220:1232	15.40 wt% CMS	1220:1232	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	5	69	theme	adhesive	854:861	arg1	strength					863:870	adhesive strength	854:870	adhesive strength	854:870	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	7	70	theme	coating	1516:1522	arg1	surface					1524:1530	the coating surface	1512:1530	the coating surface	1512:1530	The optimum coating composited with 15.40 wt% CMS shows a relatively stable rheological development, the setting time sufficient at higher than 4 h. Furthermore, CMS shows a significant positive effect on the cracking and shrinkage control due to padding effect and water retention of CMS, which results in no visible cracks on the coating surface.					
32075084	0	71	theme	Slag	73:76	arg1	Composites					98:107	Alkali-Activated Slag /Starch(CMS) Hybrid Composites	56:107	Alkali-Activated Slag /Starch(CMS) Hybrid Composites	56:107	One-Part Plastic Formable Inorganic Coating Obtain from Alkali-Activated Slag /Starch(CMS) Hybrid Composites.					
32075084	4	72	theme	rheological	490:500	arg1	viscosity					532:540	plastic viscosity	524:540	plastic viscosity	524:540	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	4	72	theme	rheological	490:500	arg1	properties					603:612	critical properties	594:612	critical properties	594:612	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	4	72	theme	rheological	490:500	arg1	thixotropy					561:570	thixotropy	561:570	thixotropy	561:570	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	4	72	theme	rheological	490:500	arg1	properties					502:511	The rheological properties	486:511	The rheological properties	486:511	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	4	72	theme	rheological	490:500	arg1	stress					549:554	yield stress	543:554	yield stress	543:554	The rheological properties, including plastic viscosity, yield stress, and thixotropy were considered to be critical properties to obtain the working requirements.					
32075084	2	73	theme	inorganic	245:253	arg1	binders					255:261	promising green and durable inorganic binders	217:261	promising green and durable inorganic binders which show potential for development as innovative coating	217:320	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	2	73	theme	inorganic	245:253	arg1	materials					197:205	Alkali-activated materials	180:205	Alkali-activated materials (AAM)	180:211	Alkali-activated materials (AAM) are promising green and durable inorganic binders which show potential for development as innovative coating.					
32075084	5	74	theme	drying	874:879	arg1	shrinkage					881:889	drying shrinkage	874:889	drying shrinkage	874:889	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	5	74	theme	drying	874:879	arg1	strengths					816:824	mechanical strengths	805:824	mechanical strengths (compressive, flexural, and adhesive strength)	805:871	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	5	74	theme	drying	874:879	arg1	properties					785:794	their hardened properties	770:794	their hardened properties	770:794	Four different mixtures were systematically investigated to obtain the optimum formulation, and then were used to study their hardened properties, such as mechanical strengths (compressive, flexural, and adhesive strength), drying shrinkage, cracking behavior, and microstructure.					
32075084	8	75	theme	strength	1557:1564	arg1	development					1566:1576	the mechanical strength development	1542:1576	the mechanical strength development	1542:1576	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
32075084	8	75	theme	strength	1557:1564	arg1	lower					1592:1596	lower	1592:1596	lower	1592:1596	Although the mechanical strength development is relatively lower than that of plain AAM, its value, adhesive strength 2.11 MPa, compressive strength 55.09 MPa, and flexural strength 8.06 MPa highly meet the requirements of a relevant standard.					
34785200	0	0	theme	composition	86:96	arg1	Extraction					0:9	Extraction	0:9	Extraction of mannoprotein from Saccharomyces cerevisiae	0:55	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	0	0	theme	composition	86:96	arg1	analysis					61:68	analysis	61:68	analysis of its chemical composition and molecular structure	61:120	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	6	1	theme	MPs	1007:1009	arg1	parts					994:998	the polysaccharide parts	975:998	the polysaccharide parts of the MPs	975:1009	After purification, the proportion of mannose in the polysaccharide parts of the MPs increased from 6-7 to 90.4-91.3%.					
34785200	9	2	theme	heating	1319:1325	arg1	time-saving					1367:1377	time-saving	1367:1377	time-saving	1367:1377	In general, the heating method to extract yeast mannoprotein is time-saving and efficient.					
34785200	9	2	theme	heating	1319:1325	arg1	method					1327:1332	the heating method	1315:1332	the heating method to extract yeast mannoprotein	1315:1362	In general, the heating method to extract yeast mannoprotein is time-saving and efficient.					
34785200	0	3	theme	chemical	77:84	arg1	composition					86:96	its chemical composition	73:96	its chemical composition	73:96	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	4	4	theme	34 kDa	775:780	arg1	weights					752:758	similar molecular weights	734:758	similar molecular weights of 59, 47, and 34 kDa	734:780	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	8	5	theme	room	1229:1232	arg1	temperature					1234:1244	room temperature	1229:1244	room temperature	1229:1244	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	0	6	theme	molecular	102:110	arg1	structure					112:120	molecular structure	102:120	molecular structure	102:120	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	5	7	theme	weight	905:910	arg1	distribution					912:923	a broader molecular weight distribution	885:923	a broader molecular weight distribution	885:923	The thermally extracted MP has a broader molecular weight distribution.					
34785200	4	8	theme	59	763:764	arg1	weights					752:758	similar molecular weights	734:758	similar molecular weights of 59, 47, and 34 kDa	734:780	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	8	9	theme	characteristic	1272:1285	arg1	intensity					1292:1300	a higher protein characteristic peak intensity	1255:1300	a higher protein characteristic peak intensity	1255:1300	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	1	10	theme	functional	241:250	arg1	structure					252:260	their functional structure	235:260	their functional structure	235:260	The purpose of this study was to screen the method with the highest yield of mannoprotein (MP) without damaging their functional structure.					
34785200	2	11	theme	extracts	494:501	arg1	ratio					367:371	the mannose/protein ratio	347:371	the mannose/protein ratio	347:371	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	2	11	theme	extracts	494:501	arg1	compositions					439:450	monosaccharide and amino acid compositions	409:450	compositions	439:450	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	2	11	theme	extracts	494:501	arg1	distribution					395:406	distribution	395:406	distribution	395:406	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	2	11	theme	extracts	494:501	arg1	structures					467:476	secondary structures	457:476	secondary structures of the three MP extracts	457:501	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	2	11	theme	extracts	494:501	arg1	weight					384:389	molecular weight	374:389	molecular weight	374:389	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	8	12	contain	had	1160:1162	arg2	characteristics					1193:1207	similar secondary structural characteristics	1164:1207	similar secondary structural characteristics	1164:1207	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	8	12	contain	had	1160:1162	arg1	MP					1157:1158	The thermally extracted MP	1133:1158	The thermally extracted MP	1133:1158	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	2	13	theme	recovery	267:274	arg1	rates					276:280	The recovery rates	263:280	The recovery rates of the extracts, protein, and mannose	263:318	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	2	14	theme	MP	491:492	arg1	extracts					494:501	the three MP extracts	481:501	the three MP extracts	481:501	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	4	15	theme	47	767:768	arg1	weights					752:758	similar molecular weights	734:758	similar molecular weights of 59, 47, and 34 kDa	734:780	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	6	16	theme	polysaccharide	979:992	arg1	parts					994:998	the polysaccharide parts	975:998	the polysaccharide parts of the MPs	975:1009	After purification, the proportion of mannose in the polysaccharide parts of the MPs increased from 6-7 to 90.4-91.3%.					
34785200	0	17	theme	structure	112:120	arg1	Extraction					0:9	Extraction	0:9	Extraction of mannoprotein from Saccharomyces cerevisiae	0:55	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	0	17	theme	structure	112:120	arg1	analysis					61:68	analysis	61:68	analysis of its chemical composition and molecular structure	61:120	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	0	18	from	cerevisiae	46:55	arg1	Extraction					0:9	Extraction	0:9	Extraction of mannoprotein from Saccharomyces cerevisiae	0:55	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	0	18	from	cerevisiae	46:55	arg1	analysis					61:68	analysis	61:68	analysis of its chemical composition and molecular structure	61:120	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	5	19	theme	broader	887:893	arg1	distribution					912:923	a broader molecular weight distribution	885:923	a broader molecular weight distribution	885:923	The thermally extracted MP has a broader molecular weight distribution.					
34785200	5	20	contain	has	881:883	arg1	MP					878:879	The thermally extracted MP	854:879	The thermally extracted MP	854:879	The thermally extracted MP has a broader molecular weight distribution.					
34785200	5	20	contain	has	881:883	arg2	distribution					912:923	a broader molecular weight distribution	885:923	a broader molecular weight distribution	885:923	The thermally extracted MP has a broader molecular weight distribution.					
34785200	7	21	theme	hot-extracted	1085:1097	arg1	170.07 mg/g					1103:1113	170.07 mg/g	1103:1113	170.07 mg/g	1103:1113	The essential amino acid content of the hot-extracted MP (170.07 mg/g) was the highest.					
34785200	7	21	theme	hot-extracted	1085:1097	arg1	MP					1099:1100	the hot-extracted MP	1081:1100	the hot-extracted MP (170.07 mg/g)	1081:1114	The essential amino acid content of the hot-extracted MP (170.07 mg/g) was the highest.					
34785200	2	22	theme	amino	428:432	arg1	compositions					439:450	monosaccharide and amino acid compositions	409:450	compositions	439:450	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	2	23	theme	secondary	457:465	arg1	structures					467:476	secondary structures	457:476	secondary structures of the three MP extracts	457:501	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	6	24	from	proportion	950:959	arg1	parts					994:998	the polysaccharide parts	975:998	the polysaccharide parts of the MPs	975:1009	After purification, the proportion of mannose in the polysaccharide parts of the MPs increased from 6-7 to 90.4-91.3%.					
34785200	3	25	theme	MP	542:543	arg1	extracts					545:552	MP extracts	542:552	The MP extracts recovery rate prepared by the thermal method	538:597	The MP extracts recovery rate prepared by the thermal method was significantly higher (35.89%) than those obtained using fermentation (31.66%) and SDS treatment (19.77%).					
34785200	9	26	from	general	1306:1312	arg1	time-saving					1367:1377	time-saving	1367:1377	time-saving	1367:1377	In general, the heating method to extract yeast mannoprotein is time-saving and efficient.					
34785200	9	26	from	general	1306:1312	arg1	method					1327:1332	the heating method	1315:1332	the heating method to extract yeast mannoprotein	1315:1362	In general, the heating method to extract yeast mannoprotein is time-saving and efficient.					
34785200	2	27	theme	mannose/protein	351:365	arg1	ratio					367:371	the mannose/protein ratio	347:371	the mannose/protein ratio	347:371	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	7	28	theme	amino	1059:1063	arg1	content					1070:1076	The essential amino acid content	1045:1076	The essential amino acid content of the hot-extracted MP (170.07 mg/g)	1045:1114	The essential amino acid content of the hot-extracted MP (170.07 mg/g) was the highest.					
34785200	7	28	theme	amino	1059:1063	arg1	highest					1124:1130	highest	1124:1130	highest	1124:1130	The essential amino acid content of the hot-extracted MP (170.07 mg/g) was the highest.					
34785200	2	29	theme	extracts	289:296	arg1	rates					276:280	The recovery rates	263:280	The recovery rates of the extracts, protein, and mannose	263:318	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	2	30	theme	monosaccharide	409:422	arg1	compositions					439:450	monosaccharide and amino acid compositions	409:450	compositions	439:450	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	4	31	theme	different	824:832	arg1	methods					845:851	the different extraction methods	820:851	the different extraction methods	820:851	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	8	32	theme	peak	1287:1290	arg1	intensity					1292:1300	a higher protein characteristic peak intensity	1255:1300	a higher protein characteristic peak intensity	1255:1300	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	1	33	theme	highest	183:189	arg1	yield					191:195	the highest yield	179:195	the highest yield of mannoprotein (MP)	179:216	The purpose of this study was to screen the method with the highest yield of mannoprotein (MP) without damaging their functional structure.					
34785200	0	34	theme	mannoprotein	14:25	arg1	Extraction					0:9	Extraction	0:9	Extraction of mannoprotein from Saccharomyces cerevisiae	0:55	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	0	34	theme	mannoprotein	14:25	arg1	analysis					61:68	analysis	61:68	analysis of its chemical composition and molecular structure	61:120	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	3	35	theme	SDS	685:687	arg1	treatment					689:697	SDS treatment	685:697	SDS treatment (19.77%)	685:706	The MP extracts recovery rate prepared by the thermal method was significantly higher (35.89%) than those obtained using fermentation (31.66%) and SDS treatment (19.77%).					
34785200	3	35	theme	SDS	685:687	arg1	%					705:705	19.77%	700:705	19.77%	700:705	The MP extracts recovery rate prepared by the thermal method was significantly higher (35.89%) than those obtained using fermentation (31.66%) and SDS treatment (19.77%).					
34785200	2	36	theme	extraction	518:527	arg1	methods					529:535	the extraction methods	514:535	the extraction methods	514:535	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	5	37	theme	extracted	868:876	arg1	MP					878:879	The thermally extracted MP	854:879	The thermally extracted MP	854:879	The thermally extracted MP has a broader molecular weight distribution.					
34785200	6	38	theme	mannose	964:970	arg1	proportion					950:959	the proportion	946:959	the proportion of mannose in the polysaccharide parts of the MPs	946:1009	After purification, the proportion of mannose in the polysaccharide parts of the MPs increased from 6-7 to 90.4-91.3%.					
34785200	7	39	theme	essential	1049:1057	arg1	content					1070:1076	The essential amino acid content	1045:1076	The essential amino acid content of the hot-extracted MP (170.07 mg/g)	1045:1114	The essential amino acid content of the hot-extracted MP (170.07 mg/g) was the highest.					
34785200	7	39	theme	essential	1049:1057	arg1	highest					1124:1130	highest	1124:1130	highest	1124:1130	The essential amino acid content of the hot-extracted MP (170.07 mg/g) was the highest.					
34785200	8	40	theme	secondary	1172:1180	arg1	characteristics					1193:1207	similar secondary structural characteristics	1164:1207	similar secondary structural characteristics	1164:1207	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	0	41	theme	Saccharomyces	32:44	arg1	cerevisiae					46:55	Saccharomyces cerevisiae	32:55	Saccharomyces cerevisiae	32:55	Extraction of mannoprotein from Saccharomyces cerevisiae and analysis of its chemical composition and molecular structure.					
34785200	4	42	theme	extraction	834:843	arg1	methods					845:851	the different extraction methods	820:851	the different extraction methods	820:851	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	4	43	theme	molecular	742:750	arg1	weights					752:758	similar molecular weights	734:758	similar molecular weights of 59, 47, and 34 kDa	734:780	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	1	44	theme	mannoprotein	200:211	arg1	yield					191:195	the highest yield	179:195	the highest yield of mannoprotein (MP)	179:216	The purpose of this study was to screen the method with the highest yield of mannoprotein (MP) without damaging their functional structure.					
34785200	8	45	contain	had	1251:1253	arg2	intensity					1292:1300	a higher protein characteristic peak intensity	1255:1300	a higher protein characteristic peak intensity	1255:1300	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	8	45	contain	had	1251:1253	arg1	MP					1157:1158	The thermally extracted MP	1133:1158	The thermally extracted MP	1133:1158	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	8	46	theme	protein	1264:1270	arg1	intensity					1292:1300	a higher protein characteristic peak intensity	1255:1300	a higher protein characteristic peak intensity	1255:1300	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	3	47	theme	recovery	554:561	arg1	rate					563:566	The MP extracts recovery rate	538:566	The MP extracts recovery rate prepared by the thermal method	538:597	The MP extracts recovery rate prepared by the thermal method was significantly higher (35.89%) than those obtained using fermentation (31.66%) and SDS treatment (19.77%).					
34785200	3	47	theme	recovery	554:561	arg1	higher					617:622	higher	617:622	higher	617:622	The MP extracts recovery rate prepared by the thermal method was significantly higher (35.89%) than those obtained using fermentation (31.66%) and SDS treatment (19.77%).					
34785200	8	48	theme	higher	1257:1262	arg1	intensity					1292:1300	a higher protein characteristic peak intensity	1255:1300	a higher protein characteristic peak intensity	1255:1300	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	2	49	theme	mannose	312:318	arg1	rates					276:280	The recovery rates	263:280	The recovery rates of the extracts, protein, and mannose	263:318	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	2	50	theme	acid	434:437	arg1	compositions					439:450	monosaccharide and amino acid compositions	409:450	compositions	439:450	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	7	51	theme	MP	1099:1100	arg1	content					1070:1076	The essential amino acid content	1045:1076	The essential amino acid content of the hot-extracted MP (170.07 mg/g)	1045:1114	The essential amino acid content of the hot-extracted MP (170.07 mg/g) was the highest.					
34785200	7	51	theme	MP	1099:1100	arg1	highest					1124:1130	highest	1124:1130	highest	1124:1130	The essential amino acid content of the hot-extracted MP (170.07 mg/g) was the highest.					
34785200	8	52	theme	structural	1182:1191	arg1	characteristics					1193:1207	similar secondary structural characteristics	1164:1207	similar secondary structural characteristics	1164:1207	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	6	53	dep	90.4-91.3	1033:1041	arg1	to					1030:1031	to	1030:1031	to	1030:1031	After purification, the proportion of mannose in the polysaccharide parts of the MPs increased from 6-7 to 90.4-91.3%.					
34785200	5	54	theme	molecular	895:903	arg1	distribution					912:923	a broader molecular weight distribution	885:923	a broader molecular weight distribution	885:923	The thermally extracted MP has a broader molecular weight distribution.					
34785200	8	55	theme	extracted	1147:1155	arg1	MP					1157:1158	The thermally extracted MP	1133:1158	The thermally extracted MP	1133:1158	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	8	56	theme	similar	1164:1170	arg1	characteristics					1193:1207	similar secondary structural characteristics	1164:1207	similar secondary structural characteristics	1164:1207	The thermally extracted MP had similar secondary structural characteristics to that isolated at room temperature, and had a higher protein characteristic peak intensity.					
34785200	9	57	from	time-saving	1367:1377	arg1	general					1306:1312	general	1306:1312	general	1306:1312	In general, the heating method to extract yeast mannoprotein is time-saving and efficient.					
34785200	4	58	theme	protein	715:721	arg1	bands					723:727	Three protein bands	709:727	Three protein bands with similar molecular weights of 59, 47, and 34 kDa	709:780	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	2	59	theme	protein	299:305	arg1	rates					276:280	The recovery rates	263:280	The recovery rates of the extracts, protein, and mannose	263:318	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	4	60	located	detected	787:794	arg1	MPs					803:805	the MPs	799:805	the MPs obtained via the different extraction methods	799:851	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	4	60	located	detected	787:794	arg2	bands					723:727	Three protein bands	709:727	Three protein bands with similar molecular weights of 59, 47, and 34 kDa	709:780	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	3	61	dep	higher	617:622	arg1	%					630:630	35.89%	625:630	35.89%	625:630	The MP extracts recovery rate prepared by the thermal method was significantly higher (35.89%) than those obtained using fermentation (31.66%) and SDS treatment (19.77%).					
34785200	2	62	theme	molecular	374:382	arg1	weight					384:389	molecular weight	374:389	molecular weight	374:389	The recovery rates of the extracts, protein, and mannose were determined along with the mannose/protein ratio, molecular weight and distribution, monosaccharide and amino acid compositions, and secondary structures of the three MP extracts to compare the extraction methods.					
34785200	4	63	theme	similar	734:740	arg1	weights					752:758	similar molecular weights	734:758	similar molecular weights of 59, 47, and 34 kDa	734:780	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	1	64	theme	study	143:147	arg1	purpose					127:133	The purpose	123:133	The purpose of this study	123:147	The purpose of this study was to screen the method with the highest yield of mannoprotein (MP) without damaging their functional structure.					
34785200	9	65	theme	extract	1337:1343	arg1	mannoprotein					1351:1362	extract yeast mannoprotein	1337:1362	extract yeast mannoprotein	1337:1362	In general, the heating method to extract yeast mannoprotein is time-saving and efficient.					
34785200	7	66	theme	acid	1065:1068	arg1	content					1070:1076	The essential amino acid content	1045:1076	The essential amino acid content of the hot-extracted MP (170.07 mg/g)	1045:1114	The essential amino acid content of the hot-extracted MP (170.07 mg/g) was the highest.					
34785200	7	66	theme	acid	1065:1068	arg1	highest					1124:1130	highest	1124:1130	highest	1124:1130	The essential amino acid content of the hot-extracted MP (170.07 mg/g) was the highest.					
34785200	4	67	with	bands	723:727	arg1	weights					752:758	similar molecular weights	734:758	similar molecular weights of 59, 47, and 34 kDa	734:780	Three protein bands with similar molecular weights of 59, 47, and 34 kDa were detected in the MPs obtained via the different extraction methods.					
34785200	9	68	theme	yeast	1345:1349	arg1	mannoprotein					1351:1362	extract yeast mannoprotein	1337:1362	extract yeast mannoprotein	1337:1362	In general, the heating method to extract yeast mannoprotein is time-saving and efficient.					
34785200	3	69	theme	thermal	584:590	arg1	method					592:597	the thermal method	580:597	the thermal method	580:597	The MP extracts recovery rate prepared by the thermal method was significantly higher (35.89%) than those obtained using fermentation (31.66%) and SDS treatment (19.77%).					
34785200	3	70	theme	extracts	545:552	arg1	rate					563:566	The MP extracts recovery rate	538:566	The MP extracts recovery rate prepared by the thermal method	538:597	The MP extracts recovery rate prepared by the thermal method was significantly higher (35.89%) than those obtained using fermentation (31.66%) and SDS treatment (19.77%).					
34785200	3	70	theme	extracts	545:552	arg1	higher					617:622	higher	617:622	higher	617:622	The MP extracts recovery rate prepared by the thermal method was significantly higher (35.89%) than those obtained using fermentation (31.66%) and SDS treatment (19.77%).					
32503355	2	0	theme	degradation	559:569	arg1	products					571:578	degradation products	559:578	degradation products	559:578	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	4	1	theme	secondary	992:1000	arg1	by-products					1002:1012	secondary by-products	992:1012	secondary by-products	992:1012	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	2	2	theme	valuable	665:672	arg1	chemicals					674:682	valuable chemicals	665:682	valuable chemicals	665:682	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	5	3	theme	access	1123:1128	arg1	separation					1091:1100	the separation	1087:1100	the separation of lignin and easier access of enzymes to the cellulosic sugars	1087:1164	The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process.					
32503355	7	4	theme	component	1388:1396	arg1	analysis					1398:1405	The principal component analysis	1374:1405	The principal component analysis	1374:1405	The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.					
32503355	7	5	theme	waste	1503:1507	arg1	compositions					1476:1487	the chemical compositions	1463:1487	the chemical compositions of vine-shoot waste for different varieties	1463:1531	The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.					
32503355	5	6	theme	enzymes	1133:1139	arg1	lignin					1105:1110	lignin	1105:1110	lignin	1105:1110	The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process.					
32503355	5	6	theme	enzymes	1133:1139	arg1	access					1123:1128	easier access	1116:1128	easier access of enzymes to the cellulosic sugars	1116:1164	The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process.					
32503355	1	7	theme	simultaneous	397:408	arg1	saccharification					410:425	simultaneous saccharification	397:425	simultaneous saccharification	397:425	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	1	7	theme	simultaneous	397:408	arg1	conditions					321:330	the optimal operating conditions	299:330	the optimal operating conditions of the autohydrolysis pretreatment	299:365	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	3	8	theme	vine-shoot	726:735	arg1	wastes					737:742	the vine-shoot wastes	722:742	the vine-shoot wastes	722:742	The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed.					
32503355	1	9	from	production	173:182	arg1	wastes					256:261	lignocellulosic vineyard cutting wastes	223:261	lignocellulosic vineyard cutting wastes	223:261	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	7	10	theme	main	1432:1435	arg1	parameters					1437:1446	the main parameters	1428:1446	the main parameters that influence the chemical compositions of vine-shoot waste for different varieties	1428:1531	The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.					
32503355	2	11	theme	rich	598:601	arg1	solids					590:595	spent solids	584:595	spent solids (rich in cellulose and lignin)	584:626	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	0	12	theme	Simultaneous	106:117	arg1	Saccharification					119:134	Simultaneous Saccharification	106:134	Simultaneous Saccharification	106:134	Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation.					
32503355	5	13	theme	lignin	1105:1110	arg1	separation					1091:1100	the separation	1087:1100	the separation of lignin and easier access of enzymes to the cellulosic sugars	1087:1164	The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process.					
32503355	2	14	contain	containing	511:520	arg2	mixture					531:537	a mixture	529:537	a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin)	529:626	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	2	14	contain	containing	511:520	arg1	liquors					503:509	liquors	503:509	liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol	503:697	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	1	15	theme	optimal	303:309	arg1	delignification					377:391	chlorite delignification	368:391	chlorite delignification	368:391	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	1	15	theme	optimal	303:309	arg1	saccharification					410:425	simultaneous saccharification	397:425	simultaneous saccharification	397:425	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	1	15	theme	optimal	303:309	arg1	conditions					321:330	the optimal operating conditions	299:330	the optimal operating conditions of the autohydrolysis pretreatment	299:365	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	2	16	theme	solids	590:595	arg1	mixture					531:537	a mixture	529:537	a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin)	529:626	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	3	17	theme	10	782:783	arg1	min					785:787	min	785:787	min	785:787	The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed.					
32503355	4	18	theme	liquid	886:891	arg1	fraction					893:900	The resulted liquid fraction	873:900	The resulted liquid fraction	873:900	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	2	19	theme	wastes	484:489	arg1	autohydrolysis					455:468	The autohydrolysis	451:468	The autohydrolysis of vine-shoot wastes	451:489	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	2	20	theme	spent	584:588	arg1	solids					590:595	spent solids	584:595	spent solids (rich in cellulose and lignin)	584:626	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	5	21	theme	easier	1116:1121	arg1	access					1123:1128	easier access	1116:1128	easier access of enzymes to the cellulosic sugars	1116:1164	The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process.					
32503355	4	22	theme	resulted	877:884	arg1	fraction					893:900	The resulted liquid fraction	873:900	The resulted liquid fraction	873:900	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	5	23	theme	SSF	1205:1207	arg1	process					1209:1215	the SSF process	1201:1215	the SSF process	1201:1215	The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process.					
32503355	1	24	theme	second-generation	189:205	arg1	bioethanol					207:216	a second-generation bioethanol	187:216	a second-generation bioethanol from lignocellulosic vineyard cutting wastes	187:261	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	0	25	from	Waste	36:40	arg1	Production					11:20	Bioethanol Production	0:20	Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation.	0:152	Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation.					
32503355	1	26	theme	bioethanol	207:216	arg1	production					173:182	the production	169:182	the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes	169:261	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	0	27	theme	Bioethanol	0:9	arg1	Production					11:20	Bioethanol Production	0:20	Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation.	0:152	Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation.					
32503355	1	28	theme	operating	311:319	arg1	delignification					377:391	chlorite delignification	368:391	chlorite delignification	368:391	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	1	28	theme	operating	311:319	arg1	saccharification					410:425	simultaneous saccharification	397:425	simultaneous saccharification	397:425	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	1	28	theme	operating	311:319	arg1	conditions					321:330	the optimal operating conditions	299:330	the optimal operating conditions of the autohydrolysis pretreatment	299:365	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	6	29	theme	bioethanol	1230:1239	arg1	production					1241:1250	The maximum bioethanol production	1218:1250	The maximum bioethanol production (6%)	1218:1255	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	6	29	theme	bioethanol	1230:1239	arg1	%					1254:1254	6%	1253:1254	6%	1253:1254	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	4	30	theme	hemicellulosic	912:925	arg1	sugars					927:932	hemicellulosic sugars	912:932	hemicellulosic sugars	912:932	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	4	30	theme	hemicellulosic	912:925	arg1	mixture					939:945	a mixture	937:945	a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products	937:1012	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	0	31	theme	Vineyard	27:34	arg1	Waste					36:40	Vineyard Waste	27:40	Vineyard Waste	27:40	Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation.					
32503355	6	32	theme	chlorite	1298:1305	arg1	delignification					1307:1321	chlorite delignification	1298:1321	chlorite delignification	1298:1321	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	6	33	theme	maximum	1222:1228	arg1	production					1241:1250	The maximum bioethanol production	1218:1250	The maximum bioethanol production (6%)	1218:1255	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	6	33	theme	maximum	1222:1228	arg1	%					1254:1254	6%	1253:1254	6%	1253:1254	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	1	34	theme	lignocellulosic	223:237	arg1	wastes					256:261	lignocellulosic vineyard cutting wastes	223:261	lignocellulosic vineyard cutting wastes	223:261	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	3	35	theme	min	785:787	arg1	time					799:802	10 min residence time	782:802	10 min residence time	782:802	The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed.					
32503355	7	36	theme	chemical	1467:1474	arg1	compositions					1476:1487	the chemical compositions	1463:1487	the chemical compositions of vine-shoot waste for different varieties	1463:1531	The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.					
32503355	1	37	theme	vineyard	239:246	arg1	wastes					256:261	lignocellulosic vineyard cutting wastes	223:261	lignocellulosic vineyard cutting wastes	223:261	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	2	38	theme	monosaccharides	542:556	arg1	mixture					531:537	a mixture	529:537	a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin)	529:626	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	3	39	theme	residence	789:797	arg1	time					799:802	10 min residence time	782:802	10 min residence time	782:802	The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed.					
32503355	4	40	theme	beta	964:967	arg1	anomers					979:985	alpha (α) and beta (β) sugar anomers	950:985	alpha (α) and beta (β) sugar anomers	950:985	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	2	41	theme	vine-shoot	473:482	arg1	wastes					484:489	vine-shoot wastes	473:489	vine-shoot wastes	473:489	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	5	42	theme	solid	1019:1023	arg1	fraction					1025:1032	The solid fraction	1015:1032	The solid fraction	1015:1032	The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process.					
32503355	1	43	theme	cutting	248:254	arg1	wastes					256:261	lignocellulosic vineyard cutting wastes	223:261	lignocellulosic vineyard cutting wastes	223:261	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	4	44	theme	by-products	1002:1012	arg1	sugars					927:932	hemicellulosic sugars	912:932	hemicellulosic sugars	912:932	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	4	44	theme	by-products	1002:1012	arg1	mixture					939:945	a mixture	937:945	a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products	937:1012	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	1	45	theme	autohydrolysis	339:352	arg1	pretreatment					354:365	the autohydrolysis pretreatment	335:365	the autohydrolysis pretreatment	335:365	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	6	46	theme	solid	1353:1357	arg1	loading					1359:1365	10% solid loading	1349:1365	10% solid loading	1349:1365	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	6	46	theme	solid	1353:1357	arg1	°C					1345:1346	37 °C	1342:1346	37 °C	1342:1346	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	7	47	theme	principal	1378:1386	arg1	analysis					1398:1405	The principal component analysis	1374:1405	The principal component analysis	1374:1405	The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.					
32503355	3	48	theme	the	809:811	arg1	composition					846:856	the resulted solid and liquid phases composition	809:856	the resulted solid and liquid phases composition	809:856	The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed.					
32503355	6	49	theme	%	1351:1351	arg1	loading					1359:1365	10% solid loading	1349:1365	10% solid loading	1349:1365	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	6	49	theme	%	1351:1351	arg1	°C					1345:1346	37 °C	1342:1346	37 °C	1342:1346	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	1	50	theme	pretreatment	354:365	arg1	delignification					377:391	chlorite delignification	368:391	chlorite delignification	368:391	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	1	50	theme	pretreatment	354:365	arg1	saccharification					410:425	simultaneous saccharification	397:425	simultaneous saccharification	397:425	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	1	50	theme	pretreatment	354:365	arg1	conditions					321:330	the optimal operating conditions	299:330	the optimal operating conditions of the autohydrolysis pretreatment	299:365	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	2	51	theme	potential	634:642	arg1	utility					644:650	potential utility	634:650	potential utility in obtaining valuable chemicals and bioethanol	634:697	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	4	52	theme	anomers	979:985	arg1	sugars					927:932	hemicellulosic sugars	912:932	hemicellulosic sugars	912:932	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	4	52	theme	anomers	979:985	arg1	mixture					939:945	a mixture	937:945	a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products	937:1012	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	1	53	from	wastes	256:261	arg1	bioethanol					207:216	a second-generation bioethanol	187:216	a second-generation bioethanol from lignocellulosic vineyard cutting wastes	187:261	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	1	53	from	wastes	256:261	arg1	production					173:182	the production	169:182	the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes	169:261	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	0	54	theme	Autohydrolysis	45:58	arg1	Pretreatment					60:71	Autohydrolysis Pretreatment	45:71	Autohydrolysis Pretreatment	45:71	Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation.					
32503355	5	55	theme	cellulosic	1148:1157	arg1	sugars					1159:1164	the cellulosic sugars	1144:1164	the cellulosic sugars	1144:1164	The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process.					
32503355	4	56	theme	sugar	973:977	arg1	anomers					979:985	alpha (α) and beta (β) sugar anomers	950:985	alpha (α) and beta (β) sugar anomers	950:985	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	1	57	theme	chlorite	368:375	arg1	delignification					377:391	chlorite delignification	368:391	chlorite delignification	368:391	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	1	57	theme	chlorite	368:375	arg1	conditions					321:330	the optimal operating conditions	299:330	the optimal operating conditions of the autohydrolysis pretreatment	299:365	In this paper, the production of a second-generation bioethanol from lignocellulosic vineyard cutting wastes was investigated in order to define the optimal operating conditions of the autohydrolysis pretreatment, chlorite delignification and simultaneous saccharification and fermentation (SSF).					
32503355	7	58	theme	different	1513:1521	arg1	varieties					1523:1531	different varieties	1513:1531	different varieties	1513:1531	The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.					
32503355	5	59	theme	sodium	1060:1065	arg1	method					1076:1081	the sodium chlorite method	1056:1081	the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars	1056:1164	The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process.					
32503355	4	60	contain	contained	902:910	arg1	fraction					893:900	The resulted liquid fraction	873:900	The resulted liquid fraction	873:900	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	4	60	contain	contained	902:910	arg2	mixture					939:945	a mixture	937:945	a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products	937:1012	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	4	60	contain	contained	902:910	arg2	sugars					927:932	hemicellulosic sugars	912:932	hemicellulosic sugars	912:932	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	2	61	from	cellulose	606:614	arg1	rich					598:601	rich	598:601	rich	598:601	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	4	62	theme	alpha	950:954	arg1	anomers					979:985	alpha (α) and beta (β) sugar anomers	950:985	alpha (α) and beta (β) sugar anomers	950:985	The resulted liquid fraction contained hemicellulosic sugars as a mixture of alpha (α) and beta (β) sugar anomers, and secondary by-products.					
32503355	2	63	from	rich	598:601	arg1	lignin					620:625	lignin	620:625	lignin	620:625	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	2	63	from	rich	598:601	arg1	cellulose					606:614	cellulose	606:614	cellulose	606:614	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	6	64	theme	SSF	1327:1329	arg1	process					1331:1337	SSF process	1327:1337	SSF process	1327:1337	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	5	65	theme	chlorite	1067:1074	arg1	method					1076:1081	the sodium chlorite method	1056:1081	the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars	1056:1164	The solid fraction was delignified using the sodium chlorite method for the separation of lignin and easier access of enzymes to the cellulosic sugars, and then, converted to ethanol by the SSF process.					
32503355	0	66	theme	Chlorite	77:84	arg1	Delignification					86:100	Chlorite Delignification	77:100	Chlorite Delignification	77:100	Bioethanol Production from Vineyard Waste by Autohydrolysis Pretreatment and Chlorite Delignification via Simultaneous Saccharification and Fermentation.					
32503355	3	67	theme	wastes	737:742	arg1	autohydrolysis					704:717	The autohydrolysis	700:717	The autohydrolysis of the vine-shoot wastes	700:742	The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed.					
32503355	7	68	theme	vine-shoot	1492:1501	arg1	waste					1503:1507	vine-shoot waste	1492:1507	vine-shoot waste	1492:1507	The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.					
32503355	6	69	theme	10	1349:1350	arg1	%					1351:1351	%	1351:1351	%	1351:1351	The maximum bioethanol production (6%) was obtained by autohydrolysis (165 °C), chlorite delignification and SSF process at 37 °C, 10% solid loading, 72 h.					
32503355	2	70	from	lignin	620:625	arg1	rich					598:601	rich	598:601	rich	598:601	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	2	71	theme	products	571:578	arg1	mixture					531:537	a mixture	529:537	a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin)	529:626	The autohydrolysis of vine-shoot wastes resulted in liquors containing mainly a mixture of monosaccharides, degradation products and spent solids (rich in cellulose and lignin), with potential utility in obtaining valuable chemicals and bioethanol.					
32503355	3	72	theme	liquid	832:837	arg1	phases					839:844	solid and liquid phases	822:844	solid and liquid phases	822:844	The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed.					
32503355	7	73	used	used	1411:1414	arg2	analysis					1398:1405	The principal component analysis	1374:1405	The principal component analysis	1374:1405	The principal component analysis was used to identify the main parameters that influence the chemical compositions of vine-shoot waste for different varieties.					
32503355	3	74	theme	solid	822:826	arg1	phases					839:844	solid and liquid phases	822:844	solid and liquid phases	822:844	The autohydrolysis of the vine-shoot wastes was carried out at 165 and 180 °C for 10 min residence time, and the resulted solid and liquid phases composition were analysed.					
34312648	5	0	theme	red	800:802	arg1	CR-TPSA					840:846	CR-TPSA	840:846	CR-TPSA	840:846	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	5	0	theme	red	800:802	arg1	derivative					828:837	a Congo red residue-conjugated TPSA derivative	792:837	a Congo red residue-conjugated TPSA derivative (CR-TPSA)	792:847	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	5	1	theme	residue-conjugated	804:821	arg1	CR-TPSA					840:846	CR-TPSA	840:846	CR-TPSA	840:846	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	5	1	theme	residue-conjugated	804:821	arg1	derivative					828:837	a Congo red residue-conjugated TPSA derivative	792:837	a Congo red residue-conjugated TPSA derivative (CR-TPSA)	792:847	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	1	2	theme	them	190:193	arg1	few					183:185	few	183:185	few	183:185	Tea polysaccharides exhibit multiple important bioactivities, but very few of them can be absorbed through the small intestine.					
34312648	7	3	theme	complexation	1202:1213	arg1	processes					1215:1223	the CR-TPSA and VB12-DETA-Gly-II complexation processes	1169:1223	processes	1215:1223	The results indicated that the electrostatic interaction could play a major role during the CR-TPSA and VB12-DETA-Gly-II complexation processes.					
34312648	2	4	theme	vitamin	310:316	arg1	derivative					342:351	a cationic vitamin B12-conjugated glycogen derivative	299:351	a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly)	299:407	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	2	5	theme	B12-conjugated	318:331	arg1	derivative					342:351	a cationic vitamin B12-conjugated glycogen derivative	299:351	a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly)	299:407	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	4	6	theme	zeta	637:640	arg1	potentials					642:651	positive zeta potentials	628:651	positive zeta potentials	628:651	The TPSA/VB12-DETA-Gly complexed nanoparticles were prepared, which showed positive zeta potentials and were irregular spherical nanoparticles in the sizes of 50-100 nm.					
34312648	2	7	theme	cationic	301:308	arg1	derivative					342:351	a cationic vitamin B12-conjugated glycogen derivative	299:351	a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly)	299:407	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	4	8	theme	positive	628:635	arg1	potentials					642:651	positive zeta potentials	628:651	positive zeta potentials	628:651	The TPSA/VB12-DETA-Gly complexed nanoparticles were prepared, which showed positive zeta potentials and were irregular spherical nanoparticles in the sizes of 50-100 nm.					
34312648	5	9	theme	TPSA	786:789	arg1	properties					772:781	the fluorescence and UV-vis absorption properties	733:781	properties	772:781	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	5	9	theme	TPSA	786:789	arg1	fluorescence					737:748	the fluorescence and UV-vis absorption properties	733:781	fluorescence	737:748	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	6	10	theme	VB12-DETA-Gly	938:950	arg1	derivatives					952:962	the VB12-DETA-Gly derivatives	934:962	the VB12-DETA-Gly derivatives	934:962	The interactions and complexation mechanism between the CR-TPSA and the VB12-DETA-Gly derivatives were investigated using fluorescence spectroscopy, resonance light scattering spectroscopy, and UV-vis spectroscopy.					
34312648	4	11	theme	spherical	672:680	arg1	nanoparticles					682:694	irregular spherical nanoparticles	662:694	irregular spherical nanoparticles in the sizes of 50-100 nm	662:720	The TPSA/VB12-DETA-Gly complexed nanoparticles were prepared, which showed positive zeta potentials and were irregular spherical nanoparticles in the sizes of 50-100 nm.					
34312648	2	12	theme	FTIR	449:452	arg1	NMR					458:460	1H NMR	455:460	1H NMR	455:460	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	6	13	theme	UV-vis	1060:1065	arg1	spectroscopy					1067:1078	UV-vis spectroscopy	1060:1078	UV-vis spectroscopy	1060:1078	The interactions and complexation mechanism between the CR-TPSA and the VB12-DETA-Gly derivatives were investigated using fluorescence spectroscopy, resonance light scattering spectroscopy, and UV-vis spectroscopy.					
34312648	7	14	theme	VB12-DETA-Gly-II	1185:1200	arg1	processes					1215:1223	the CR-TPSA and VB12-DETA-Gly-II complexation processes	1169:1223	processes	1215:1223	The results indicated that the electrostatic interaction could play a major role during the CR-TPSA and VB12-DETA-Gly-II complexation processes.					
34312648	9	15	theme	polysaccharides	1500:1514	arg1	efficacy					1484:1491	the absorption efficacy	1469:1491	the absorption efficacy of tea polysaccharides	1469:1514	Therefore, they exhibit potential for enhancing the absorption efficacy of tea polysaccharides through the small intestinal mucosa.					
34312648	1	16	theme	Tea	112:114	arg1	polysaccharides					116:130	Tea polysaccharides	112:130	Tea polysaccharides	112:130	Tea polysaccharides exhibit multiple important bioactivities, but very few of them can be absorbed through the small intestine.					
34312648	4	17	theme	nm	719:720	arg1	sizes					703:707	the sizes	699:707	the sizes of 50-100 nm	699:720	The TPSA/VB12-DETA-Gly complexed nanoparticles were prepared, which showed positive zeta potentials and were irregular spherical nanoparticles in the sizes of 50-100 nm.					
34312648	7	18	theme	major	1151:1155	arg1	role					1157:1160	a major role	1149:1160	a major role	1149:1160	The results indicated that the electrostatic interaction could play a major role during the CR-TPSA and VB12-DETA-Gly-II complexation processes.					
34312648	8	19	theme	intestinal	1369:1378	arg1	enterocytes					1380:1390	intestinal enterocytes	1369:1390	intestinal enterocytes using the Caco-2 cell model	1369:1418	The TPSA/VB12-DETA-Gly nanoparticles were nontoxic and exhibited targeted endocytosis for the Caco-2 cells, and showed high permeation through intestinal enterocytes using the Caco-2 cell model.					
34312648	2	20	theme	UV-vis	467:472	arg1	spectroscopy					474:485	UV-vis spectroscopy	467:485	UV-vis spectroscopy	467:485	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	8	21	theme	targeted	1291:1298	arg1	endocytosis					1300:1310	targeted endocytosis	1291:1310	targeted endocytosis for the Caco-2 cells	1291:1331	The TPSA/VB12-DETA-Gly nanoparticles were nontoxic and exhibited targeted endocytosis for the Caco-2 cells, and showed high permeation through intestinal enterocytes using the Caco-2 cell model.					
34312648	2	22	theme	glycogen	333:340	arg1	derivative					342:351	a cationic vitamin B12-conjugated glycogen derivative	299:351	a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly)	299:407	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	3	23	theme	tea	498:500	arg1	TPSA					518:521	TPSA	518:521	TPSA	518:521	An acidic tea polysaccharide (TPSA) was isolated from green tea.					
34312648	3	23	theme	tea	498:500	arg1	polysaccharide					502:515	An acidic tea polysaccharide	488:515	An acidic tea polysaccharide (TPSA)	488:522	An acidic tea polysaccharide (TPSA) was isolated from green tea.					
34312648	8	24	theme	Caco-2	1320:1325	arg1	cells					1327:1331	the Caco-2 cells	1316:1331	the Caco-2 cells	1316:1331	The TPSA/VB12-DETA-Gly nanoparticles were nontoxic and exhibited targeted endocytosis for the Caco-2 cells, and showed high permeation through intestinal enterocytes using the Caco-2 cell model.					
34312648	9	25	theme	absorption	1473:1482	arg1	efficacy					1484:1491	the absorption efficacy	1469:1491	the absorption efficacy of tea polysaccharides	1469:1514	Therefore, they exhibit potential for enhancing the absorption efficacy of tea polysaccharides through the small intestinal mucosa.					
34312648	1	26	theme	multiple	140:147	arg1	bioactivities					159:171	multiple important bioactivities	140:171	multiple important bioactivities	140:171	Tea polysaccharides exhibit multiple important bioactivities, but very few of them can be absorbed through the small intestine.					
34312648	4	27	from	nanoparticles	682:694	arg1	sizes					703:707	the sizes	699:707	the sizes of 50-100 nm	699:720	The TPSA/VB12-DETA-Gly complexed nanoparticles were prepared, which showed positive zeta potentials and were irregular spherical nanoparticles in the sizes of 50-100 nm.					
34312648	1	28	theme	small	223:227	arg1	intestine					229:237	the small intestine	219:237	the small intestine	219:237	Tea polysaccharides exhibit multiple important bioactivities, but very few of them can be absorbed through the small intestine.					
34312648	5	29	theme	TPSA	823:826	arg1	CR-TPSA					840:846	CR-TPSA	840:846	CR-TPSA	840:846	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	5	29	theme	TPSA	823:826	arg1	derivative					828:837	a Congo red residue-conjugated TPSA derivative	792:837	a Congo red residue-conjugated TPSA derivative (CR-TPSA)	792:847	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	6	30	theme	scattering	1031:1040	arg1	spectroscopy					1042:1053	resonance light scattering spectroscopy	1015:1053	resonance light scattering spectroscopy	1015:1053	The interactions and complexation mechanism between the CR-TPSA and the VB12-DETA-Gly derivatives were investigated using fluorescence spectroscopy, resonance light scattering spectroscopy, and UV-vis spectroscopy.					
34312648	5	31	theme	UV-vis	754:759	arg1	properties					772:781	the fluorescence and UV-vis absorption properties	733:781	properties	772:781	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	8	32	theme	cell	1409:1412	arg1	model					1414:1418	the Caco-2 cell model	1398:1418	the Caco-2 cell model	1398:1418	The TPSA/VB12-DETA-Gly nanoparticles were nontoxic and exhibited targeted endocytosis for the Caco-2 cells, and showed high permeation through intestinal enterocytes using the Caco-2 cell model.					
34312648	2	33	theme	absorption	255:264	arg1	efficacy					266:273	the absorption efficacy	251:273	the absorption efficacy of tea polysaccharides	251:296	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	2	34	theme	1H	455:456	arg1	NMR					458:460	1H NMR	455:460	1H NMR	455:460	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	6	35	theme	light	1025:1029	arg1	scattering					1031:1040	resonance light scattering	1015:1040	resonance light scattering spectroscopy	1015:1053	The interactions and complexation mechanism between the CR-TPSA and the VB12-DETA-Gly derivatives were investigated using fluorescence spectroscopy, resonance light scattering spectroscopy, and UV-vis spectroscopy.					
34312648	5	36	theme	absorption	761:770	arg1	properties					772:781	the fluorescence and UV-vis absorption properties	733:781	properties	772:781	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	9	37	theme	intestinal	1534:1543	arg1	mucosa					1545:1550	the small intestinal mucosa	1524:1550	the small intestinal mucosa	1524:1550	Therefore, they exhibit potential for enhancing the absorption efficacy of tea polysaccharides through the small intestinal mucosa.					
34312648	3	38	theme	acidic	491:496	arg1	TPSA					518:521	TPSA	518:521	TPSA	518:521	An acidic tea polysaccharide (TPSA) was isolated from green tea.					
34312648	3	38	theme	acidic	491:496	arg1	polysaccharide					502:515	An acidic tea polysaccharide	488:515	An acidic tea polysaccharide (TPSA)	488:522	An acidic tea polysaccharide (TPSA) was isolated from green tea.					
34312648	0	39	theme	vitamin	68:74	arg1	derivative					100:109	cationic vitamin B12-conjugated glycogen derivative	59:109	cationic vitamin B12-conjugated glycogen derivative	59:109	Nanoparticles composed of the tea polysaccharide-complexed cationic vitamin B12-conjugated glycogen derivative.					
34312648	6	40	theme	resonance	1015:1023	arg1	scattering					1031:1040	resonance light scattering	1015:1040	resonance light scattering spectroscopy	1015:1053	The interactions and complexation mechanism between the CR-TPSA and the VB12-DETA-Gly derivatives were investigated using fluorescence spectroscopy, resonance light scattering spectroscopy, and UV-vis spectroscopy.					
34312648	5	41	theme	Congo	794:798	arg1	CR-TPSA					840:846	CR-TPSA	840:846	CR-TPSA	840:846	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	5	41	theme	Congo	794:798	arg1	derivative					828:837	a Congo red residue-conjugated TPSA derivative	792:837	a Congo red residue-conjugated TPSA derivative (CR-TPSA)	792:847	To enable the fluorescence and UV-vis absorption properties of TPSA, a Congo red residue-conjugated TPSA derivative (CR-TPSA) was synthesized.					
34312648	1	42	theme	important	149:157	arg1	bioactivities					159:171	multiple important bioactivities	140:171	multiple important bioactivities	140:171	Tea polysaccharides exhibit multiple important bioactivities, but very few of them can be absorbed through the small intestine.					
34312648	0	43	theme	cationic	59:66	arg1	derivative					100:109	cationic vitamin B12-conjugated glycogen derivative	59:109	cationic vitamin B12-conjugated glycogen derivative	59:109	Nanoparticles composed of the tea polysaccharide-complexed cationic vitamin B12-conjugated glycogen derivative.					
34312648	4	44	theme	complexed	576:584	arg1	nanoparticles					586:598	The TPSA/VB12-DETA-Gly complexed nanoparticles	553:598	The TPSA/VB12-DETA-Gly complexed nanoparticles	553:598	The TPSA/VB12-DETA-Gly complexed nanoparticles were prepared, which showed positive zeta potentials and were irregular spherical nanoparticles in the sizes of 50-100 nm.					
34312648	6	45	theme	complexation	887:898	arg1	mechanism					900:908	complexation mechanism	887:908	complexation mechanism	887:908	The interactions and complexation mechanism between the CR-TPSA and the VB12-DETA-Gly derivatives were investigated using fluorescence spectroscopy, resonance light scattering spectroscopy, and UV-vis spectroscopy.					
34312648	8	46	theme	TPSA/VB12-DETA-Gly	1230:1247	arg1	nontoxic					1268:1275	nontoxic	1268:1275	nontoxic	1268:1275	The TPSA/VB12-DETA-Gly nanoparticles were nontoxic and exhibited targeted endocytosis for the Caco-2 cells, and showed high permeation through intestinal enterocytes using the Caco-2 cell model.					
34312648	8	46	theme	TPSA/VB12-DETA-Gly	1230:1247	arg1	nanoparticles					1249:1261	The TPSA/VB12-DETA-Gly nanoparticles	1226:1261	The TPSA/VB12-DETA-Gly nanoparticles	1226:1261	The TPSA/VB12-DETA-Gly nanoparticles were nontoxic and exhibited targeted endocytosis for the Caco-2 cells, and showed high permeation through intestinal enterocytes using the Caco-2 cell model.					
34312648	7	47	theme	electrostatic	1112:1124	arg1	interaction					1126:1136	the electrostatic interaction	1108:1136	the electrostatic interaction	1108:1136	The results indicated that the electrostatic interaction could play a major role during the CR-TPSA and VB12-DETA-Gly-II complexation processes.					
34312648	2	48	theme	polysaccharides	282:296	arg1	efficacy					266:273	the absorption efficacy	251:273	the absorption efficacy of tea polysaccharides	251:296	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	3	49	attach	isolated	528:535	arg2	polysaccharide					502:515	An acidic tea polysaccharide	488:515	An acidic tea polysaccharide (TPSA)	488:522	An acidic tea polysaccharide (TPSA) was isolated from green tea.					
34312648	3	49	attach	isolated	528:535	arg2	TPSA					518:521	TPSA	518:521	TPSA	518:521	An acidic tea polysaccharide (TPSA) was isolated from green tea.					
34312648	3	49	attach	isolated	528:535	arg1	tea					548:550	green tea	542:550	green tea	542:550	An acidic tea polysaccharide (TPSA) was isolated from green tea.					
34312648	2	50	theme	diethylenetriamine	365:382	arg1	residues					384:391	the diethylenetriamine residues	361:391	the diethylenetriamine residues (VB12-DETA-Gly)	361:407	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	2	50	theme	diethylenetriamine	365:382	arg1	VB12-DETA-Gly					394:406	VB12-DETA-Gly	394:406	VB12-DETA-Gly	394:406	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	2	51	theme	tea	278:280	arg1	polysaccharides					282:296	tea polysaccharides	278:296	tea polysaccharides	278:296	To enhance the absorption efficacy of tea polysaccharides, a cationic vitamin B12-conjugated glycogen derivative bearing the diethylenetriamine residues (VB12-DETA-Gly) was synthesized and characterized using FTIR, 1H NMR, and UV-vis spectroscopy.					
34312648	8	52	theme	high	1345:1348	arg1	permeation					1350:1359	high permeation	1345:1359	high permeation through intestinal enterocytes using the Caco-2 cell model	1345:1418	The TPSA/VB12-DETA-Gly nanoparticles were nontoxic and exhibited targeted endocytosis for the Caco-2 cells, and showed high permeation through intestinal enterocytes using the Caco-2 cell model.					
34312648	9	53	theme	small	1528:1532	arg1	mucosa					1545:1550	the small intestinal mucosa	1524:1550	the small intestinal mucosa	1524:1550	Therefore, they exhibit potential for enhancing the absorption efficacy of tea polysaccharides through the small intestinal mucosa.					
34312648	0	54	theme	glycogen	91:98	arg1	derivative					100:109	cationic vitamin B12-conjugated glycogen derivative	59:109	cationic vitamin B12-conjugated glycogen derivative	59:109	Nanoparticles composed of the tea polysaccharide-complexed cationic vitamin B12-conjugated glycogen derivative.					
34312648	8	55	theme	Caco-2	1402:1407	arg1	model					1414:1418	the Caco-2 cell model	1398:1418	the Caco-2 cell model	1398:1418	The TPSA/VB12-DETA-Gly nanoparticles were nontoxic and exhibited targeted endocytosis for the Caco-2 cells, and showed high permeation through intestinal enterocytes using the Caco-2 cell model.					
34312648	4	56	theme	irregular	662:670	arg1	nanoparticles					682:694	irregular spherical nanoparticles	662:694	irregular spherical nanoparticles in the sizes of 50-100 nm	662:720	The TPSA/VB12-DETA-Gly complexed nanoparticles were prepared, which showed positive zeta potentials and were irregular spherical nanoparticles in the sizes of 50-100 nm.					
34312648	0	57	theme	B12-conjugated	76:89	arg1	derivative					100:109	cationic vitamin B12-conjugated glycogen derivative	59:109	cationic vitamin B12-conjugated glycogen derivative	59:109	Nanoparticles composed of the tea polysaccharide-complexed cationic vitamin B12-conjugated glycogen derivative.					
34312648	6	58	theme	fluorescence	988:999	arg1	spectroscopy					1001:1012	fluorescence spectroscopy	988:1012	fluorescence spectroscopy	988:1012	The interactions and complexation mechanism between the CR-TPSA and the VB12-DETA-Gly derivatives were investigated using fluorescence spectroscopy, resonance light scattering spectroscopy, and UV-vis spectroscopy.					
34312648	9	59	theme	tea	1496:1498	arg1	polysaccharides					1500:1514	tea polysaccharides	1496:1514	tea polysaccharides	1496:1514	Therefore, they exhibit potential for enhancing the absorption efficacy of tea polysaccharides through the small intestinal mucosa.					
32610625	7	0	theme	isopropanol	1274:1284	arg1	precipitation					1286:1298	fractional isopropanol precipitation	1263:1298	fractional isopropanol precipitation	1263:1298	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	6	1	theme	conformation	1023:1034	arg1	Estimation					997:1006	Estimation	997:1006	Estimation	997:1006	Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (α), confirmed that acid hydrolysis influenced the polysaccharides' conformation.					
32610625	0	2	theme	Monosaccharide	58:71	arg1	Composition					73:83	Monosaccharide Composition	58:83	Monosaccharide Composition	58:83	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	1	3	theme	partial	169:175	arg1	hydrolysis					182:191	partial acid hydrolysis	169:191	partial acid hydrolysis	169:191	The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated.					
32610625	6	4	theme	polymer	1015:1021	arg1	conformation					1023:1034	the polymer conformation	1011:1034	the polymer conformation	1011:1034	Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (α), confirmed that acid hydrolysis influenced the polysaccharides' conformation.					
32610625	0	5	from	Influence	0:8	arg1	Conformation					90:101	Conformation	90:101	Conformation	90:101	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	0	5	from	Influence	0:8	arg1	Dispersity					46:55	Dispersity	46:55	Dispersity	46:55	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	0	5	from	Influence	0:8	arg1	Composition					73:83	Monosaccharide Composition	58:83	Monosaccharide Composition	58:83	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	0	5	from	Influence	0:8	arg1	Size					40:43	Size	40:43	Size	40:43	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	2	6	dep	h.	368:369	arg1	added					409:413	added	409:413	added	409:413	Polysaccharides were treated at 50 °C with hydrochloric acid for 3-48 h. Portions of isopropanol (i-PrOH) were added sequentially to the hydrolyzates, resulting in fractions that were collected by centrifugation.					
32610625	7	7	theme	altered	1440:1446	arg1	composition					1456:1466	slightly altered overall composition	1431:1466	slightly altered overall composition	1431:1466	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	1	8	theme	xyloglucan	269:278	arg1	properties					222:231	the physical and chemical properties	196:231	the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan	196:278	The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated.					
32610625	7	9	theme	dilute	1235:1240	arg1	solution					1242:1249	dilute solution	1235:1249	dilute solution	1235:1249	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	3	10	theme	weight-average	551:564	arg1	weight					576:581	weight-average molecular weight	551:581	weight-average molecular weight (M)	551:585	As expected, a significant reduction of weight-average molecular weight (M) was observed with increasing hydrolysis time.					
32610625	3	10	theme	weight-average	551:564	arg1	M					584:584	M	584:584	M	584:584	As expected, a significant reduction of weight-average molecular weight (M) was observed with increasing hydrolysis time.					
32610625	5	11	theme	relative	877:884	arg1	amount					886:891	the relative amount	873:891	the relative amount of side chains	873:906	The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected.					
32610625	5	11	theme	relative	877:884	arg1	chains					901:906	side chains	896:906	side chains	896:906	The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected.					
32610625	4	12	theme	polymer	707:713	arg1	fraction					715:722	at least one polymer fraction	694:722	at least one polymer fraction	694:722	Fractional precipitation was successfully applied to collect at least one polymer fraction with dispersity (Đ) close to one for each polysaccharide.					
32610625	1	13	from	effect	159:164	arg1	properties					222:231	the physical and chemical properties	196:231	the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan	196:278	The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated.					
32610625	3	14	theme	significant	526:536	arg1	reduction					538:546	a significant reduction	524:546	a significant reduction of weight-average molecular weight (M)	524:585	As expected, a significant reduction of weight-average molecular weight (M) was observed with increasing hydrolysis time.					
32610625	5	15	theme	galactomannan	931:943	arg1	exception					918:926	the exception	914:926	the exception of galactomannan, where the composition remained largely unaffected	914:994	The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected.					
32610625	1	16	theme	acid	177:180	arg1	hydrolysis					182:191	partial acid hydrolysis	169:191	partial acid hydrolysis	169:191	The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated.					
32610625	7	17	theme	polysaccharides	1340:1354	arg1	polysaccharides					1340:1354	polysaccharides	1340:1354	polysaccharides	1340:1354	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	7	17	theme	polysaccharides	1340:1354	arg1	variety					1329:1335	a variety	1327:1335	a variety of polysaccharides	1327:1354	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	7	18	theme	weight	1400:1405	arg1	materials					1367:1375	materials	1367:1375	materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation	1367:1483	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	1	19	theme	hydrolysis	182:191	arg1	effect					159:164	The effect	155:164	The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan	155:278	The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated.					
32610625	7	20	theme	overall	1448:1454	arg1	composition					1456:1466	slightly altered overall composition	1431:1466	slightly altered overall composition	1431:1466	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	6	21	theme	coefficient	1098:1108	arg1	evaluation					1057:1066	evaluation	1057:1066	evaluation of the Mark-Houwink parameter coefficient (α)	1057:1112	Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (α), confirmed that acid hydrolysis influenced the polysaccharides' conformation.					
32610625	2	22	theme	isopropanol	383:393	arg1	Portions					371:378	Portions	371:378	Portions of isopropanol (i-PrOH)	371:402	Polysaccharides were treated at 50 °C with hydrochloric acid for 3-48 h. Portions of isopropanol (i-PrOH) were added sequentially to the hydrolyzates, resulting in fractions that were collected by centrifugation.					
32610625	0	23	theme	Acid	21:24	arg1	Hydrolysis					26:35	Partial Acid Hydrolysis	13:35	Partial Acid Hydrolysis	13:35	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	0	24	theme	Water-Soluble	124:136	arg1	Polysaccharides					138:152	Linearly-Branched Water-Soluble Polysaccharides	106:152	Linearly-Branched Water-Soluble Polysaccharides	106:152	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	3	25	theme	molecular	566:574	arg1	weight					576:581	weight-average molecular weight	551:581	weight-average molecular weight (M)	551:585	As expected, a significant reduction of weight-average molecular weight (M) was observed with increasing hydrolysis time.					
32610625	3	25	theme	molecular	566:574	arg1	M					584:584	M	584:584	M	584:584	As expected, a significant reduction of weight-average molecular weight (M) was observed with increasing hydrolysis time.					
32610625	1	26	theme	physical	200:207	arg1	properties					222:231	the physical and chemical properties	196:231	the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan	196:278	The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated.					
32610625	0	27	theme	Partial	13:19	arg1	Hydrolysis					26:35	Partial Acid Hydrolysis	13:35	Partial Acid Hydrolysis	13:35	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	0	28	theme	Linearly-Branched	106:122	arg1	Polysaccharides					138:152	Linearly-Branched Water-Soluble Polysaccharides	106:152	Linearly-Branched Water-Soluble Polysaccharides	106:152	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	3	29	theme	weight	576:581	arg1	reduction					538:546	a significant reduction	524:546	a significant reduction of weight-average molecular weight (M)	524:585	As expected, a significant reduction of weight-average molecular weight (M) was observed with increasing hydrolysis time.					
32610625	4	30	theme	Fractional	633:642	arg1	precipitation					644:656	Fractional precipitation	633:656	Fractional precipitation	633:656	Fractional precipitation was successfully applied to collect at least one polymer fraction with dispersity (Đ) close to one for each polysaccharide.					
32610625	6	31	from	Estimation	997:1006	arg1	solution					1039:1046	solution	1039:1046	solution	1039:1046	Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (α), confirmed that acid hydrolysis influenced the polysaccharides' conformation.					
32610625	7	32	from	treatment	1222:1230	arg1	solution					1242:1249	dilute solution	1235:1249	dilute solution	1235:1249	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	7	33	theme	dispersity	1415:1424	arg1	materials					1367:1375	materials	1367:1375	materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation	1367:1483	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	5	34	theme	side	896:899	arg1	chains					901:906	side chains	896:906	side chains	896:906	The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected.					
32610625	1	35	theme	chemical	213:220	arg1	properties					222:231	the physical and chemical properties	196:231	the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan	196:278	The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated.					
32610625	0	36	theme	Hydrolysis	26:35	arg1	Influence					0:8	Influence	0:8	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.	0:153	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	0	37	theme	Polysaccharides	138:152	arg1	Conformation					90:101	Conformation	90:101	Conformation	90:101	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	0	37	theme	Polysaccharides	138:152	arg1	Dispersity					46:55	Dispersity	46:55	Dispersity	46:55	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	0	37	theme	Polysaccharides	138:152	arg1	Composition					73:83	Monosaccharide Composition	58:83	Monosaccharide Composition	58:83	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	0	37	theme	Polysaccharides	138:152	arg1	Size					40:43	Size	40:43	Size	40:43	Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides.					
32610625	5	38	theme	composition	801:811	arg1	analysis					813:820	The monosaccharide composition analysis	782:820	The monosaccharide composition analysis	782:820	The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected.					
32610625	6	39	theme	parameter	1088:1096	arg1	coefficient					1098:1108	the Mark-Houwink parameter coefficient	1071:1108	the Mark-Houwink parameter coefficient (α)	1071:1112	Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (α), confirmed that acid hydrolysis influenced the polysaccharides' conformation.					
32610625	6	39	theme	parameter	1088:1096	arg1	α					1111:1111	α	1111:1111	α	1111:1111	Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (α), confirmed that acid hydrolysis influenced the polysaccharides' conformation.					
32610625	7	40	theme	low	1411:1413	arg1	dispersity					1415:1424	low dispersity	1411:1424	low dispersity	1411:1424	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	7	41	theme	decreased	1380:1388	arg1	weight					1400:1405	decreased molecular weight	1380:1405	decreased molecular weight	1380:1405	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	7	42	theme	molecular	1390:1398	arg1	weight					1400:1405	decreased molecular weight	1380:1405	decreased molecular weight	1380:1405	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	5	43	theme	monosaccharide	786:799	arg1	analysis					813:820	The monosaccharide composition analysis	782:820	The monosaccharide composition analysis	782:820	The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected.					
32610625	2	44	theme	hydrochloric	341:352	arg1	acid					354:357	hydrochloric acid	341:357	hydrochloric acid for 3-48 h. Portions of isopropanol (i-PrOH) were added sequentially to the hydrolyzates, resulting in fractions that were collected by centrifugation	341:508	Polysaccharides were treated at 50 °C with hydrochloric acid for 3-48 h. Portions of isopropanol (i-PrOH) were added sequentially to the hydrolyzates, resulting in fractions that were collected by centrifugation.					
32610625	7	45	with	materials	1367:1375	arg1	conformation					1472:1483	conformation	1472:1483	conformation	1472:1483	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	7	45	with	materials	1367:1375	arg1	composition					1456:1466	slightly altered overall composition	1431:1466	slightly altered overall composition	1431:1466	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	5	46	theme	partial	838:844	arg1	hydrolysis					846:855	the partial hydrolysis	834:855	the partial hydrolysis	834:855	The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected.					
32610625	6	47	theme	acid	1130:1133	arg1	hydrolysis					1135:1144	acid hydrolysis	1130:1144	acid hydrolysis	1130:1144	Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (α), confirmed that acid hydrolysis influenced the polysaccharides' conformation.					
32610625	1	48	theme	galactomannan	236:248	arg1	properties					222:231	the physical and chemical properties	196:231	the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan	196:278	The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated.					
32610625	7	49	dep	extendible	1313:1322	arg1	treatment					1222:1230	acid treatment	1217:1230	acid treatment in dilute solution followed by fractional isopropanol precipitation	1217:1298	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	7	49	dep	extendible	1313:1322	arg1	method					1305:1310	a method	1303:1310	a method	1303:1310	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	6	50	theme	Mark-Houwink	1075:1086	arg1	coefficient					1098:1108	the Mark-Houwink parameter coefficient	1071:1108	the Mark-Houwink parameter coefficient (α)	1071:1112	Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (α), confirmed that acid hydrolysis influenced the polysaccharides' conformation.					
32610625	6	50	theme	Mark-Houwink	1075:1086	arg1	α					1111:1111	α	1111:1111	α	1111:1111	Estimation of the polymer conformation in solution, through evaluation of the Mark-Houwink parameter coefficient (α), confirmed that acid hydrolysis influenced the polysaccharides' conformation.					
32610625	5	51	theme	chains	901:906	arg1	amount					886:891	the relative amount	873:891	the relative amount of side chains	873:906	The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected.					
32610625	5	51	theme	chains	901:906	arg1	chains					901:906	side chains	896:906	side chains	896:906	The monosaccharide composition analysis showed that the partial hydrolysis usually lowered the relative amount of side chains, with the exception of galactomannan, where the composition remained largely unaffected.					
32610625	7	52	theme	fractional	1263:1272	arg1	precipitation					1286:1298	fractional isopropanol precipitation	1263:1298	fractional isopropanol precipitation	1263:1298	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	7	53	theme	acid	1217:1220	arg1	treatment					1222:1230	acid treatment	1217:1230	acid treatment in dilute solution followed by fractional isopropanol precipitation	1217:1298	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	7	53	theme	acid	1217:1220	arg1	method					1305:1310	a method	1303:1310	a method	1303:1310	It was demonstrated that acid treatment in dilute solution followed by fractional isopropanol precipitation is a method, extendible to a variety of polysaccharides, to obtain materials of decreased molecular weight and low dispersity with slightly altered overall composition and conformation.					
32610625	3	54	theme	hydrolysis	616:625	arg1	time					627:630	hydrolysis time	616:630	hydrolysis time	616:630	As expected, a significant reduction of weight-average molecular weight (M) was observed with increasing hydrolysis time.					
32610625	1	55	theme	arabinoxylan	251:262	arg1	properties					222:231	the physical and chemical properties	196:231	the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan	196:278	The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated.					
32414843	0	0	theme	Toxoplasma	81:90	arg1	gondii					92:97	Toxoplasma gondii	81:97	Toxoplasma gondii	81:97	A terminal α3-galactose modification regulates an E3 ubiquitin ligase subunit in Toxoplasma gondii.					
32414843	1	1	theme	hydroxylation	322:334	arg1	effect					312:317	The full effect	303:317	The full effect of hydroxylation	303:334	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	7	2	theme	Skp1	1894:1897	arg1	glycoregulation					1899:1913	Skp1 glycoregulation	1894:1913	Skp1 glycoregulation	1894:1913	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	1	3	theme	ubiquitin	150:158	arg1	ligases					160:166	E3 Skp1/Cullin-1/F-box protein ubiquitin ligases	119:166	E3 Skp1/Cullin-1/F-box protein ubiquitin ligases	119:166	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	7	4	theme	compatible	1709:1718	arg1	glycan					1720:1725	a distinct but structurally compatible glycan	1681:1725	a distinct but structurally compatible glycan in Dictyostelium	1681:1742	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	5	5	theme	fifth	1214:1218	arg1	important					1229:1237	important	1229:1237	important	1229:1237	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	5	5	theme	fifth	1214:1218	arg1	sugar					1220:1224	The fifth sugar	1210:1224	The fifth sugar	1210:1224	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	4	6	theme	Toxoplasma	909:918	arg1	enzyme					920:925	the Toxoplasma enzyme	905:925	the Toxoplasma enzyme	905:925	However, the Toxoplasma enzyme catalyzes formation of a Galα1,3Glcα linkage rather than the Glcα1,4Glcα linkage formed by glycogenin.					
32414843	1	7	theme	Skp1	436:439	arg1	structure					441:449	Skp1 structure	436:449	Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes	436:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	1	8	theme	ligases	160:166	arg1	Skp1					100:103	Skp1	100:103	Skp1	100:103	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	1	8	theme	ligases	160:166	arg1	subunit					108:114	a subunit	106:114	a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases	106:166	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	7	9	theme	convergent	1768:1777	arg1	evolution					1779:1787	convergent evolution	1768:1787	convergent evolution	1768:1787	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	1	10	theme	social	240:245	arg1	Dictyostelium					254:266	the social amoeba Dictyostelium	236:266	the social amoeba Dictyostelium	236:266	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	7	11	from	Toxoplasma	1607:1616	arg1	substitution					1567:1578	The substitution	1563:1578	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium	1563:1742	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	7	11	from	Toxoplasma	1607:1616	arg1	case					1760:1763	a remarkable case	1747:1763	a remarkable case	1747:1763	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	7	12	theme	terminal	1829:1836	arg1	α-galactose					1838:1848	the terminal α-galactose	1825:1848	the terminal α-galactose	1825:1848	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	5	13	theme	slow-growth	1279:1289	arg1	phenotype					1291:1299	the slow-growth phenotype	1275:1299	the slow-growth phenotype of gat1Δ parasites	1275:1318	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	1	14	theme	amoeba	247:252	arg1	Dictyostelium					254:266	the social amoeba Dictyostelium	236:266	the social amoeba Dictyostelium	236:266	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	7	15	from	substitution	1567:1578	arg1	Pythium					1622:1628	Pythium	1622:1628	Pythium	1622:1628	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	7	15	from	substitution	1567:1578	arg1	Toxoplasma					1607:1616	Toxoplasma	1607:1616	Toxoplasma	1607:1616	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	3	16	from	yeast	877:881	arg1	prime					849:853	prime	849:853	prime	849:853	To define the final sugar and its linkage, here we identified the glycosyltransferase that completes the glycan and found that it is closely related to glycogenin, an enzyme that may prime glycogen synthesis in yeast and animals.					
32414843	1	17	theme	Dictyostelium	254:266	arg1	regulation					222:231	O2 regulation	219:231	O2 regulation of the social amoeba Dictyostelium	219:266	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	1	17	theme	Dictyostelium	254:266	arg1	gondii					296:301	the parasite Toxoplasma gondii	272:301	the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes	272:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	4	18	theme	linkage	964:970	arg1	formation					937:945	formation	937:945	formation of a Galα1,3Glcα linkage rather than the Glcα1,4Glcα linkage formed by glycogenin	937:1027	However, the Toxoplasma enzyme catalyzes formation of a Galα1,3Glcα linkage rather than the Glcα1,4Glcα linkage formed by glycogenin.					
32414843	2	19	theme	model	659:663	arg1	testing					621:627	testing	621:627	testing of the conformational control model	621:663	In Toxoplasma, the presence of a contrasting penultimate sugar assembled by a different glycosyltransferase enables testing of the conformational control model.					
32414843	7	20	theme	distinct	1683:1690	arg1	glycan					1720:1725	a distinct but structurally compatible glycan	1681:1725	a distinct but structurally compatible glycan in Dictyostelium	1681:1742	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	6	21	theme	packing	1522:1528	arg1	interactions					1530:1541	nonpolar packing interactions	1513:1541	nonpolar packing interactions of the fifth sugar	1513:1560	Computational analyses indicated that, despite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar.					
32414843	4	22	theme	Galα1,3Glcα	952:962	arg1	linkage					964:970	a Galα1,3Glcα linkage	950:970	a Galα1,3Glcα linkage	950:970	However, the Toxoplasma enzyme catalyzes formation of a Galα1,3Glcα linkage rather than the Glcα1,4Glcα linkage formed by glycogenin.					
32414843	1	23	theme	hydroxyproline	365:378	arg1	modification					345:356	modification	345:356	modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes	345:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	2	24	theme	control	651:657	arg1	model					659:663	the conformational control model	632:663	the conformational control model	632:663	In Toxoplasma, the presence of a contrasting penultimate sugar assembled by a different glycosyltransferase enables testing of the conformational control model.					
32414843	7	25	theme	α-galactose	1838:1848	arg1	importance					1811:1820	the importance	1807:1820	the importance of the terminal α-galactose	1807:1848	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	3	26	from	animals	887:893	arg1	prime					849:853	prime	849:853	prime	849:853	To define the final sugar and its linkage, here we identified the glycosyltransferase that completes the glycan and found that it is closely related to glycogenin, an enzyme that may prime glycogen synthesis in yeast and animals.					
32414843	0	27	theme	terminal	2:9	arg1	modification					24:35	A terminal α3-galactose modification	0:35	A terminal α3-galactose modification	0:35	A terminal α3-galactose modification regulates an E3 ubiquitin ligase subunit in Toxoplasma gondii.					
32414843	6	28	theme	Toxoplasma	1397:1406	arg1	glycan					1408:1413	the Toxoplasma glycan	1393:1413	the Toxoplasma glycan	1393:1413	Computational analyses indicated that, despite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar.					
32414843	2	29	theme	conformational	636:649	arg1	model					659:663	the conformational control model	632:663	the conformational control model	632:663	In Toxoplasma, the presence of a contrasting penultimate sugar assembled by a different glycosyltransferase enables testing of the conformational control model.					
32414843	7	30	theme	remarkable	1749:1758	arg1	substitution					1567:1578	The substitution	1563:1578	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium	1563:1742	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	7	30	theme	remarkable	1749:1758	arg1	case					1760:1763	a remarkable case	1747:1763	a remarkable case	1747:1763	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	6	31	theme	ordered	1432:1438	arg1	conformation					1440:1451	an ordered conformation	1429:1451	an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar	1429:1560	Computational analyses indicated that, despite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar.					
32414843	5	32	theme	pathogen	1185:1192	arg1	protist					1167:1173	another protist	1159:1173	another protist	1159:1173	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	5	32	theme	pathogen	1185:1192	arg1	ultimum					1202:1208	the crop pathogen Pythium ultimum	1176:1208	the crop pathogen Pythium ultimum	1176:1208	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	1	33	theme	Skp1/F-box	472:481	arg1	subcomplexes					491:502	Skp1/F-box protein subcomplexes	472:502	Skp1/F-box protein subcomplexes	472:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	3	34	from	prime	849:853	arg1	yeast					877:881	yeast	877:881	yeast	877:881	To define the final sugar and its linkage, here we identified the glycosyltransferase that completes the glycan and found that it is closely related to glycogenin, an enzyme that may prime glycogen synthesis in yeast and animals.					
32414843	3	34	from	prime	849:853	arg1	animals					887:893	animals	887:893	animals	887:893	To define the final sugar and its linkage, here we identified the glycosyltransferase that completes the glycan and found that it is closely related to glycogenin, an enzyme that may prime glycogen synthesis in yeast and animals.					
32414843	6	35	theme	Skp1	1467:1470	arg1	structure					1472:1480	Skp1 structure	1467:1480	Skp1 structure	1467:1480	Computational analyses indicated that, despite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar.					
32414843	5	36	theme	Pythium	1194:1200	arg1	protist					1167:1173	another protist	1159:1173	another protist	1159:1173	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	5	36	theme	Pythium	1194:1200	arg1	ultimum					1202:1208	the crop pathogen Pythium ultimum	1176:1208	the crop pathogen Pythium ultimum	1176:1208	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	2	37	theme	sugar	562:566	arg1	presence					524:531	the presence	520:531	the presence of a contrasting penultimate sugar assembled by a different glycosyltransferase	520:611	In Toxoplasma, the presence of a contrasting penultimate sugar assembled by a different glycosyltransferase enables testing of the conformational control model.					
32414843	1	38	theme	protein	483:489	arg1	subcomplexes					491:502	Skp1/F-box protein subcomplexes	472:502	Skp1/F-box protein subcomplexes	472:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	6	39	theme	sugar	1556:1560	arg1	interactions					1530:1541	nonpolar packing interactions	1513:1541	nonpolar packing interactions of the fifth sugar	1513:1560	Computational analyses indicated that, despite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar.					
32414843	0	40	theme	α3-galactose	11:22	arg1	modification					24:35	A terminal α3-galactose modification	0:35	A terminal α3-galactose modification	0:35	A terminal α3-galactose modification regulates an E3 ubiquitin ligase subunit in Toxoplasma gondii.					
32414843	1	41	theme	parasite	276:283	arg1	gondii					296:301	the parasite Toxoplasma gondii	272:301	the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes	272:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	2	42	theme	penultimate	550:560	arg1	sugar					562:566	a contrasting penultimate sugar	536:566	a contrasting penultimate sugar assembled by a different glycosyltransferase	536:611	In Toxoplasma, the presence of a contrasting penultimate sugar assembled by a different glycosyltransferase enables testing of the conformational control model.					
32414843	5	43	theme	glycosyltransferase	1087:1105	arg1	Gat1					1107:1110	the glycosyltransferase Gat1	1083:1110	the glycosyltransferase Gat1	1083:1110	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	5	43	theme	glycosyltransferase	1087:1105	arg1	specific					1115:1122	specific	1115:1122	specific	1115:1122	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	6	44	theme	fifth	1550:1554	arg1	sugar					1556:1560	the fifth sugar	1546:1560	the fifth sugar	1546:1560	Computational analyses indicated that, despite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar.					
32414843	1	45	theme	prolyl	186:191	arg1	hydroxylase					193:203	a prolyl hydroxylase	184:203	a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes	184:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	1	46	theme	Toxoplasma	285:294	arg1	gondii					296:301	the parasite Toxoplasma gondii	272:301	the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes	272:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	2	47	theme	contrasting	538:548	arg1	sugar					562:566	a contrasting penultimate sugar	536:566	a contrasting penultimate sugar assembled by a different glycosyltransferase	536:611	In Toxoplasma, the presence of a contrasting penultimate sugar assembled by a different glycosyltransferase enables testing of the conformational control model.					
32414843	7	48	theme	glycosyltransferases	1583:1602	arg1	substitution					1567:1578	The substitution	1563:1578	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium	1563:1742	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	7	48	theme	glycosyltransferases	1583:1602	arg1	case					1760:1763	a remarkable case	1747:1763	a remarkable case	1747:1763	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	7	49	from	glycan	1720:1725	arg1	Dictyostelium					1730:1742	Dictyostelium	1730:1742	Dictyostelium	1730:1742	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	6	50	theme	nonpolar	1513:1520	arg1	interactions					1530:1541	nonpolar packing interactions	1513:1541	nonpolar packing interactions of the fifth sugar	1513:1560	Computational analyses indicated that, despite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar.					
32414843	7	51	from	Pythium	1622:1628	arg1	substitution					1567:1578	The substitution	1563:1578	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium	1563:1742	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	7	51	from	Pythium	1622:1628	arg1	case					1760:1763	a remarkable case	1747:1763	a remarkable case	1747:1763	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	0	52	from	subunit	70:76	arg1	gondii					92:97	Toxoplasma gondii	81:97	Toxoplasma gondii	81:97	A terminal α3-galactose modification regulates an E3 ubiquitin ligase subunit in Toxoplasma gondii.					
32414843	5	53	theme	Kinetic	1030:1036	arg1	experiments					1059:1069	Kinetic and crystallographic experiments	1030:1069	Kinetic and crystallographic experiments	1030:1069	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	1	54	theme	subcomplexes	491:502	arg1	assembly					460:467	assembly	460:467	assembly of Skp1/F-box protein subcomplexes	460:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	6	55	theme	sequence	1372:1379	arg1	difference					1381:1390	the sequence difference	1368:1390	the sequence difference	1368:1390	Computational analyses indicated that, despite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar.					
32414843	0	56	theme	ubiquitin	53:61	arg1	ligase					63:68	an E3 ubiquitin ligase	47:68	an E3 ubiquitin ligase subunit in Toxoplasma gondii	47:97	A terminal α3-galactose modification regulates an E3 ubiquitin ligase subunit in Toxoplasma gondii.					
32414843	2	57	theme	different	583:591	arg1	glycosyltransferase					593:611	a different glycosyltransferase	581:611	a different glycosyltransferase	581:611	In Toxoplasma, the presence of a contrasting penultimate sugar assembled by a different glycosyltransferase enables testing of the conformational control model.					
32414843	7	58	theme	bifunctional	1646:1657	arg1	enzyme					1659:1664	an unrelated bifunctional enzyme	1633:1664	an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium	1633:1742	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	1	59	dep	gondii	296:301	arg1	requires					336:343	requires	336:343	requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes	336:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	7	60	theme	phylogenetic	1870:1881	arg1	breadth					1883:1889	the phylogenetic breadth	1866:1889	the phylogenetic breadth of Skp1 glycoregulation	1866:1913	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	0	61	theme	E3	50:51	arg1	ligase					63:68	an E3 ubiquitin ligase	47:68	an E3 ubiquitin ligase subunit in Toxoplasma gondii	47:97	A terminal α3-galactose modification regulates an E3 ubiquitin ligase subunit in Toxoplasma gondii.					
32414843	1	62	theme	full	307:310	arg1	effect					312:317	The full effect	303:317	The full effect of hydroxylation	303:334	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	5	63	theme	crystallographic	1042:1057	arg1	experiments					1059:1069	Kinetic and crystallographic experiments	1030:1069	Kinetic and crystallographic experiments	1030:1069	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	1	64	mod	modified	172:179	arg1	Skp1					100:103	Skp1	100:103	Skp1	100:103	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	1	64	mod	modified	172:179	arg1	subunit					108:114	a subunit	106:114	a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases	106:166	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	1	64	mod	modified	172:179	arg3	hydroxylase					193:203	a prolyl hydroxylase	184:203	a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes	184:502	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	3	65	theme	glycogen	855:862	arg1	synthesis					864:872	glycogen synthesis	855:872	glycogen synthesis	855:872	To define the final sugar and its linkage, here we identified the glycosyltransferase that completes the glycan and found that it is closely related to glycogenin, an enzyme that may prime glycogen synthesis in yeast and animals.					
32414843	5	66	theme	crop	1180:1183	arg1	protist					1167:1173	another protist	1159:1173	another protist	1159:1173	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	5	66	theme	crop	1180:1183	arg1	ultimum					1202:1208	the crop pathogen Pythium ultimum	1176:1208	the crop pathogen Pythium ultimum	1176:1208	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	6	67	theme	Computational	1321:1333	arg1	analyses					1335:1342	Computational analyses	1321:1342	Computational analyses	1321:1342	Computational analyses indicated that, despite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar.					
32414843	7	68	from	glycosyltransferases	1583:1602	arg1	Pythium					1622:1628	Pythium	1622:1628	Pythium	1622:1628	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	7	68	from	glycosyltransferases	1583:1602	arg1	Toxoplasma					1607:1616	Toxoplasma	1607:1616	Toxoplasma	1607:1616	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	6	69	theme	interactions	1530:1541	arg1	importance					1499:1508	the importance	1495:1508	the importance of nonpolar packing interactions of the fifth sugar	1495:1560	Computational analyses indicated that, despite the sequence difference, the Toxoplasma glycan still assumes an ordered conformation that controls Skp1 structure and revealed the importance of nonpolar packing interactions of the fifth sugar.					
32414843	7	70	theme	unrelated	1636:1644	arg1	enzyme					1659:1664	an unrelated bifunctional enzyme	1633:1664	an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium	1633:1742	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	3	71	theme	final	680:684	arg1	sugar					686:690	the final sugar	676:690	the final sugar	676:690	To define the final sugar and its linkage, here we identified the glycosyltransferase that completes the glycan and found that it is closely related to glycogenin, an enzyme that may prime glycogen synthesis in yeast and animals.					
32414843	7	72	theme	glycoregulation	1899:1913	arg1	breadth					1883:1889	the phylogenetic breadth	1866:1889	the phylogenetic breadth of Skp1 glycoregulation	1866:1913	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	1	73	theme	E3	119:120	arg1	ligases					160:166	E3 Skp1/Cullin-1/F-box protein ubiquitin ligases	119:166	E3 Skp1/Cullin-1/F-box protein ubiquitin ligases	119:166	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	5	74	theme	gat1Δ	1304:1308	arg1	parasites					1310:1318	gat1Δ parasites	1304:1318	gat1Δ parasites	1304:1318	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	5	75	theme	glycan	1243:1248	arg1	function					1250:1257	glycan function	1243:1257	glycan function	1243:1257	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	7	76	theme	evolution	1779:1787	arg1	substitution					1567:1578	The substitution	1563:1578	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium	1563:1742	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	7	76	theme	evolution	1779:1787	arg1	case					1760:1763	a remarkable case	1747:1763	a remarkable case	1747:1763	The substitution of glycosyltransferases in Toxoplasma and Pythium by an unrelated bifunctional enzyme that assembles a distinct but structurally compatible glycan in Dictyostelium is a remarkable case of convergent evolution, which emphasizes the importance of the terminal α-galactose and establishes the phylogenetic breadth of Skp1 glycoregulation.					
32414843	1	77	theme	Skp1/Cullin-1/F-box	122:140	arg1	ligases					160:166	E3 Skp1/Cullin-1/F-box protein ubiquitin ligases	119:166	E3 Skp1/Cullin-1/F-box protein ubiquitin ligases	119:166	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32414843	5	78	theme	parasites	1310:1318	arg1	phenotype					1291:1299	the slow-growth phenotype	1275:1299	the slow-growth phenotype of gat1Δ parasites	1275:1318	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	0	79	theme	ligase	63:68	arg1	subunit					70:76	an E3 ubiquitin ligase subunit	47:76	an E3 ubiquitin ligase subunit in Toxoplasma gondii	47:97	A terminal α3-galactose modification regulates an E3 ubiquitin ligase subunit in Toxoplasma gondii.					
32414843	4	80	theme	Glcα1,4Glcα	988:998	arg1	linkage					1000:1006	the Glcα1,4Glcα linkage	984:1006	formation of a Galα1,3Glcα linkage rather than the Glcα1,4Glcα linkage formed by glycogenin	937:1027	However, the Toxoplasma enzyme catalyzes formation of a Galα1,3Glcα linkage rather than the Glcα1,4Glcα linkage formed by glycogenin.					
32414843	5	81	from	Skp1	1128:1131	arg1	Toxoplasma					1136:1145	Toxoplasma	1136:1145	Toxoplasma	1136:1145	Kinetic and crystallographic experiments showed that the glycosyltransferase Gat1 is specific for Skp1 in Toxoplasma and also in another protist, the crop pathogen Pythium ultimum The fifth sugar is important for glycan function as indicated by the slow-growth phenotype of gat1Δ parasites.					
32414843	1	82	theme	protein	142:148	arg1	ligases					160:166	E3 Skp1/Cullin-1/F-box protein ubiquitin ligases	119:166	E3 Skp1/Cullin-1/F-box protein ubiquitin ligases	119:166	Skp1, a subunit of E3 Skp1/Cullin-1/F-box protein ubiquitin ligases, is modified by a prolyl hydroxylase that mediates O2 regulation of the social amoeba Dictyostelium and the parasite Toxoplasma gondii The full effect of hydroxylation requires modification of the hydroxyproline by a pentasaccharide that, in Dictyostelium, influences Skp1 structure to favor assembly of Skp1/F-box protein subcomplexes.					
32517158	4	0	theme	VEGF-induced	955:966	arg1	migration					971:979	the VEGF-induced EC migration	951:979	the VEGF-induced EC migration	951:979	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	6	1	theme	VEGFR2-related	1212:1225	arg1	functions					1227:1235	its VEGFR2-related functions	1208:1235	its VEGFR2-related functions	1208:1235	Deletion of COSMC (C1GalT1C1) revealed that the abundant mucin-type O-glycans were not required for its VEGFR2-related functions.					
32517158	4	2	theme	domain	832:837	arg1	EMCN					865:868	∆21-81 EMCN	858:868	∆21-81 EMCN	858:868	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	2	theme	domain	832:837	arg1	EMCN					882:885	∆21-121 EMCN	874:885	∆21-121 EMCN	874:885	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	2	theme	domain	832:837	arg1	mutants					850:856	the extracellular domain truncation mutants	814:856	the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN	814:885	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	3	3	theme	endothelial	676:686	arg1	EC					695:696	EC	695:696	EC	695:696	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	3	theme	endothelial	676:686	arg1	cells					688:692	human primary endothelial cells	662:692	human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA	662:756	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	4	4	theme	extracellular	818:830	arg1	EMCN					865:868	∆21-81 EMCN	858:868	∆21-81 EMCN	858:868	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	4	theme	extracellular	818:830	arg1	EMCN					882:885	∆21-121 EMCN	874:885	∆21-121 EMCN	874:885	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	4	theme	extracellular	818:830	arg1	mutants					850:856	the extracellular domain truncation mutants	814:856	the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN	814:885	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	2	5	theme	VEGFR2	326:331	arg1	internalization					334:348	vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization	259:348	vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization	259:348	We have previously shown that EMCN is necessary for vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization and downstream signaling.					
32517158	8	6	theme	minimal	1419:1425	arg1	domain					1441:1446	the minimal extracellular domain	1415:1446	the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function	1415:1498	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	4	7	theme	shortest	900:907	arg1	EMCN					924:927	the shortest mutant ∆21-161 EMCN	896:927	the shortest mutant ∆21-161 EMCN	896:927	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	7	8	from	Mutation	1238:1245	arg1	EMCN					1291:1294	∆21-121 EMCN	1283:1294	∆21-121 EMCN	1283:1294	Mutation of the two N-glycosylation sites on ∆21-121 EMCN abolished its interaction with VEGFR2 and its function in VEGFR2 internalization.					
32517158	4	9	theme	mutants	850:856	arg1	Expression					759:768	Expression	759:768	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN	759:885	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	10	theme	FL	801:802	arg1	EMCN					795:798	the mouse full-length EMCN	773:798	the mouse full-length EMCN (FL EMCN)	773:808	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	10	theme	FL	801:802	arg1	EMCN					804:807	FL EMCN	801:807	FL EMCN	801:807	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	6	11	theme	COSMC	1120:1124	arg1	Deletion					1108:1115	Deletion	1108:1115	Deletion of COSMC (C1GalT1C1)	1108:1136	Deletion of COSMC (C1GalT1C1) revealed that the abundant mucin-type O-glycans were not required for its VEGFR2-related functions.					
32517158	4	12	theme	truncation	839:848	arg1	EMCN					865:868	∆21-81 EMCN	858:868	∆21-81 EMCN	858:868	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	12	theme	truncation	839:848	arg1	EMCN					882:885	∆21-121 EMCN	874:885	∆21-121 EMCN	874:885	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	12	theme	truncation	839:848	arg1	mutants					850:856	the extracellular domain truncation mutants	814:856	the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN	814:885	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	2	13	theme	growth	280:285	arg1	factor					287:292	vascular endothelial growth factor	259:292	vascular endothelial growth factor	259:292	We have previously shown that EMCN is necessary for vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization and downstream signaling.					
32517158	7	14	from	interaction	1310:1320	arg1	internalization					1361:1375	VEGFR2 internalization	1354:1375	VEGFR2 internalization	1354:1375	Mutation of the two N-glycosylation sites on ∆21-121 EMCN abolished its interaction with VEGFR2 and its function in VEGFR2 internalization.					
32517158	1	15	theme	I	120:120	arg1	glycoprotein					136:147	the type I transmembrane glycoprotein	111:147	the type I transmembrane glycoprotein	111:147	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	15	theme	I	120:120	arg1	component					161:169	mucin-like component	150:169	mucin-like component of the endothelial cell glycocalyx	150:204	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	15	theme	I	120:120	arg1	Endomucin					91:99	Endomucin	91:99	Endomucin (EMCN)	91:106	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	4	16	theme	∆21-161	916:922	arg1	EMCN					924:927	the shortest mutant ∆21-161 EMCN	896:927	the shortest mutant ∆21-161 EMCN	896:927	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	3	17	theme	molecular	465:473	arg1	mechanism					475:483	the molecular mechanism	461:483	the molecular mechanism of EMCN	461:491	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	7	18	theme	VEGFR2	1354:1359	arg1	internalization					1361:1375	VEGFR2 internalization	1354:1375	VEGFR2 internalization	1354:1375	Mutation of the two N-glycosylation sites on ∆21-121 EMCN abolished its interaction with VEGFR2 and its function in VEGFR2 internalization.					
32517158	2	19	theme	endothelial	268:278	arg1	factor					287:292	vascular endothelial growth factor	259:292	vascular endothelial growth factor	259:292	We have previously shown that EMCN is necessary for vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization and downstream signaling.					
32517158	4	20	theme	full-length	783:793	arg1	EMCN					795:798	the mouse full-length EMCN	773:798	the mouse full-length EMCN (FL EMCN)	773:808	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	20	theme	full-length	783:793	arg1	EMCN					804:807	FL EMCN	801:807	FL EMCN	801:807	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	21	theme	EC	968:969	arg1	migration					971:979	the VEGF-induced EC migration	951:979	the VEGF-induced EC migration	951:979	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	3	22	theme	EMCN	416:419	arg1	necessary					430:438	necessary	430:438	necessary	430:438	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	22	theme	EMCN	416:419	arg1	components					402:411	the structural components	387:411	the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions	387:529	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	2	23	theme	vascular	259:266	arg1	factor					287:292	vascular endothelial growth factor	259:292	vascular endothelial growth factor	259:292	We have previously shown that EMCN is necessary for vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization and downstream signaling.					
32517158	8	24	theme	sufficient	1448:1457	arg1	domain					1441:1446	the minimal extracellular domain	1415:1446	the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function	1415:1498	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	2	25	dep	-induced	300:307	arg1	factor					287:292	vascular endothelial growth factor	259:292	vascular endothelial growth factor	259:292	We have previously shown that EMCN is necessary for vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization and downstream signaling.					
32517158	4	26	theme	tube	982:985	arg1	formation					987:995	tube formation	982:995	tube formation	982:995	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	3	27	from	functions	649:657	arg1	EC					695:696	EC	695:696	EC	695:696	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	27	from	functions	649:657	arg1	cells					688:692	human primary endothelial cells	662:692	human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA	662:756	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	2	28	theme	-induced	300:307	arg1	internalization					334:348	vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization	259:348	vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization	259:348	We have previously shown that EMCN is necessary for vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization and downstream signaling.					
32517158	3	29	theme	endogenous	708:717	arg1	EMCN					719:722	endogenous EMCN	708:722	endogenous EMCN	708:722	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	5	30	theme	VEGFR2	1084:1089	arg1	internalization					1091:1105	VEGFR2 internalization	1084:1105	VEGFR2 internalization	1084:1105	∆21-161 EMCN failed to interact with VEGFR2 and did not facilitate VEGFR2 internalization.					
32517158	7	31	with	interaction	1310:1320	arg1	VEGFR2					1327:1332	VEGFR2	1327:1332	VEGFR2	1327:1332	Mutation of the two N-glycosylation sites on ∆21-121 EMCN abolished its interaction with VEGFR2 and its function in VEGFR2 internalization.					
32517158	8	32	theme	VEGFR2-mediated	1463:1477	arg1	function					1491:1498	VEGFR2-mediated endothelial function	1463:1498	VEGFR2-mediated endothelial function	1463:1498	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	2	33	theme	downstream	354:363	arg1	signaling					365:373	downstream signaling	354:373	downstream signaling	354:373	We have previously shown that EMCN is necessary for vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization and downstream signaling.					
32517158	0	34	theme	Endomucin	16:24	arg1	Domain					40:45	the Endomucin Extracellular Domain	12:45	the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity	12:88	Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity.					
32517158	3	35	from	mechanism	475:483	arg1	functions					521:529	VEGF-induced endothelial functions	496:529	VEGF-induced endothelial functions	496:529	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	4	36	theme	EMCN	795:798	arg1	Expression					759:768	Expression	759:768	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN	759:885	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	1	37	theme	cell	190:193	arg1	glycocalyx					195:204	the endothelial cell glycocalyx	174:204	the endothelial cell glycocalyx	174:204	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	8	38	theme	important	1519:1527	arg1	role					1529:1532	an important role	1516:1532	an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity	1516:1616	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	3	39	theme	endothelial	509:519	arg1	functions					521:529	VEGF-induced endothelial functions	496:529	VEGF-induced endothelial functions	496:529	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	40	theme	VEGF-induced	624:635	arg1	functions					649:657	VEGF-induced endothelial functions	624:657	VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA	624:756	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	1	41	theme	mucin-like	150:159	arg1	glycoprotein					136:147	the type I transmembrane glycoprotein	111:147	the type I transmembrane glycoprotein	111:147	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	41	theme	mucin-like	150:159	arg1	component					161:169	mucin-like component	150:169	mucin-like component of the endothelial cell glycocalyx	150:204	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	42	theme	glycocalyx	195:204	arg1	glycoprotein					136:147	the type I transmembrane glycoprotein	111:147	the type I transmembrane glycoprotein	111:147	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	42	theme	glycocalyx	195:204	arg1	component					161:169	mucin-like component	150:169	mucin-like component of the endothelial cell glycocalyx	150:204	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	0	43	theme	Domain	40:45	arg1	Elements					0:7	Elements	0:7	Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity	0:88	Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity.					
32517158	7	44	theme	N-glycosylation	1258:1272	arg1	sites					1274:1278	the two N-glycosylation sites	1250:1278	the two N-glycosylation sites	1250:1278	Mutation of the two N-glycosylation sites on ∆21-121 EMCN abolished its interaction with VEGFR2 and its function in VEGFR2 internalization.					
32517158	4	45	dep	mutants	850:856	arg1	EMCN					865:868	∆21-81 EMCN	858:868	∆21-81 EMCN	858:868	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	45	dep	mutants	850:856	arg1	EMCN					882:885	∆21-121 EMCN	874:885	∆21-121 EMCN	874:885	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	45	dep	mutants	850:856	arg1	mutants					850:856	the extracellular domain truncation mutants	814:856	the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN	814:885	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	3	46	theme	endothelial	637:647	arg1	functions					649:657	VEGF-induced endothelial functions	624:657	VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA	624:756	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	47	theme	truncation	568:577	arg1	mutants					579:585	mouse EMCN truncation mutants	557:585	mouse EMCN truncation mutants	557:585	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	1	48	theme	endothelial	178:188	arg1	glycocalyx					195:204	the endothelial cell glycocalyx	174:204	the endothelial cell glycocalyx	174:204	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	0	49	theme	Extracellular	26:38	arg1	Domain					40:45	the Endomucin Extracellular Domain	12:45	the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity	12:88	Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity.					
32517158	3	50	theme	mutants	579:585	arg1	series					547:552	a series	545:552	a series of mouse EMCN truncation mutants	545:585	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	4	51	theme	mutant	909:914	arg1	EMCN					924:927	the shortest mutant ∆21-161 EMCN	896:927	the shortest mutant ∆21-161 EMCN	896:927	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	8	52	from	role	1529:1532	arg1	interaction					1564:1574	VEGFR2 interaction	1557:1574	VEGFR2 interaction	1557:1574	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	8	52	from	role	1529:1532	arg1	activity					1609:1616	angiogenic activity	1598:1616	angiogenic activity	1598:1616	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	8	52	from	role	1529:1532	arg1	internalization					1577:1591	internalization	1577:1591	internalization	1577:1591	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	1	53	gly	glycoprotein	136:147	arg1	glycoprotein					136:147	the type I transmembrane glycoprotein	111:147	the type I transmembrane glycoprotein	111:147	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	53	gly	glycoprotein	136:147	arg1	component					161:169	mucin-like component	150:169	mucin-like component of the endothelial cell glycocalyx	150:204	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	53	gly	glycoprotein	136:147	arg1	Endomucin					91:99	Endomucin	91:99	Endomucin (EMCN)	91:106	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	8	54	theme	extracellular	1427:1439	arg1	domain					1441:1446	the minimal extracellular domain	1415:1446	the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function	1415:1498	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	3	55	theme	mouse	557:561	arg1	mutants					579:585	mouse EMCN truncation mutants	557:585	mouse EMCN truncation mutants	557:585	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	56	theme	structural	391:400	arg1	necessary					430:438	necessary	430:438	necessary	430:438	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	56	theme	structural	391:400	arg1	components					402:411	the structural components	387:411	the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions	387:529	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	0	57	theme	Essential	47:55	arg1	Domain					40:45	the Endomucin Extracellular Domain	12:45	the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity	12:88	Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity.					
32517158	8	58	theme	angiogenic	1598:1607	arg1	activity					1609:1616	angiogenic activity	1598:1616	angiogenic activity	1598:1616	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	3	59	theme	human	662:666	arg1	EC					695:696	EC	695:696	EC	695:696	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	59	theme	human	662:666	arg1	cells					688:692	human primary endothelial cells	662:692	human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA	662:756	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	60	theme	EMCN	563:566	arg1	mutants					579:585	mouse EMCN truncation mutants	557:585	mouse EMCN truncation mutants	557:585	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	61	theme	EMCN	488:491	arg1	mechanism					475:483	the molecular mechanism	461:483	the molecular mechanism of EMCN	461:491	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	61	theme	EMCN	488:491	arg1	function					448:455	its function	444:455	its function	444:455	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	7	62	gly	N-glycosylation	1258:1272	arg2	two					1254:1256	two	1254:1256	two	1254:1256	Mutation of the two N-glycosylation sites on ∆21-121 EMCN abolished its interaction with VEGFR2 and its function in VEGFR2 internalization.					
32517158	7	62	gly	N-glycosylation	1258:1272	arg2	sites					1274:1278	the two N-glycosylation sites	1250:1278	the two N-glycosylation sites	1250:1278	Mutation of the two N-glycosylation sites on ∆21-121 EMCN abolished its interaction with VEGFR2 and its function in VEGFR2 internalization.					
32517158	8	63	theme	VEGFR2	1557:1562	arg1	interaction					1564:1574	VEGFR2 interaction	1557:1574	VEGFR2 interaction	1557:1574	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	3	64	theme	primary	668:674	arg1	EC					695:696	EC	695:696	EC	695:696	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	3	64	theme	primary	668:674	arg1	cells					688:692	human primary endothelial cells	662:692	human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA	662:756	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	6	65	theme	mucin-type	1165:1174	arg1	O-glycans					1176:1184	the abundant mucin-type O-glycans	1152:1184	the abundant mucin-type O-glycans	1152:1184	Deletion of COSMC (C1GalT1C1) revealed that the abundant mucin-type O-glycans were not required for its VEGFR2-related functions.					
32517158	3	66	from	function	448:455	arg1	functions					521:529	VEGF-induced endothelial functions	496:529	VEGF-induced endothelial functions	496:529	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	1	67	theme	type	115:118	arg1	glycoprotein					136:147	the type I transmembrane glycoprotein	111:147	the type I transmembrane glycoprotein	111:147	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	67	theme	type	115:118	arg1	component					161:169	mucin-like component	150:169	mucin-like component of the endothelial cell glycocalyx	150:204	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	67	theme	type	115:118	arg1	Endomucin					91:99	Endomucin	91:99	Endomucin (EMCN)	91:106	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	8	68	theme	endothelial	1479:1489	arg1	function					1491:1498	VEGFR2-mediated endothelial function	1463:1498	VEGFR2-mediated endothelial function	1463:1498	These results reveal ∆21-121 EMCN as the minimal extracellular domain sufficient for VEGFR2-mediated endothelial function and demonstrate an important role for N-glycosylation in VEGFR2 interaction, internalization, and angiogenic activity.					
32517158	6	69	theme	abundant	1156:1163	arg1	O-glycans					1176:1184	the abundant mucin-type O-glycans	1152:1184	the abundant mucin-type O-glycans	1152:1184	Deletion of COSMC (C1GalT1C1) revealed that the abundant mucin-type O-glycans were not required for its VEGFR2-related functions.					
32517158	2	70	theme	VEGF	309:312	arg1	internalization					334:348	vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization	259:348	vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization	259:348	We have previously shown that EMCN is necessary for vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization and downstream signaling.					
32517158	2	71	theme	receptor	314:321	arg1	internalization					334:348	vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization	259:348	vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization	259:348	We have previously shown that EMCN is necessary for vascular endothelial growth factor (VEGF)-induced VEGF receptor 2 (VEGFR2) internalization and downstream signaling.					
32517158	0	72	theme	VEGFR2	74:79	arg1	Activity					81:88	VEGF-Induced VEGFR2 Activity	61:88	VEGF-Induced VEGFR2 Activity	61:88	Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity.					
32517158	5	73	theme	∆21-161	1017:1023	arg1	EMCN					1025:1028	∆21-161 EMCN	1017:1028	∆21-161 EMCN	1017:1028	∆21-161 EMCN failed to interact with VEGFR2 and did not facilitate VEGFR2 internalization.					
32517158	4	74	theme	mouse	777:781	arg1	EMCN					795:798	the mouse full-length EMCN	773:798	the mouse full-length EMCN (FL EMCN)	773:808	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	4	74	theme	mouse	777:781	arg1	EMCN					804:807	FL EMCN	801:807	FL EMCN	801:807	Expression of the mouse full-length EMCN (FL EMCN) and the extracellular domain truncation mutants ∆21-81 EMCN and ∆21-121 EMCN, but not the shortest mutant ∆21-161 EMCN, successfully rescued the VEGF-induced EC migration, tube formation, and proliferation.					
32517158	7	75	theme	sites	1274:1278	arg1	Mutation					1238:1245	Mutation	1238:1245	Mutation of the two N-glycosylation sites on ∆21-121 EMCN	1238:1294	Mutation of the two N-glycosylation sites on ∆21-121 EMCN abolished its interaction with VEGFR2 and its function in VEGFR2 internalization.					
32517158	1	76	theme	transmembrane	122:134	arg1	glycoprotein					136:147	the type I transmembrane glycoprotein	111:147	the type I transmembrane glycoprotein	111:147	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	76	theme	transmembrane	122:134	arg1	component					161:169	mucin-like component	150:169	mucin-like component of the endothelial cell glycocalyx	150:204	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	1	76	theme	transmembrane	122:134	arg1	Endomucin					91:99	Endomucin	91:99	Endomucin (EMCN)	91:106	Endomucin (EMCN) is the type I transmembrane glycoprotein, mucin-like component of the endothelial cell glycocalyx.					
32517158	0	77	theme	VEGF-Induced	61:72	arg1	Activity					81:88	VEGF-Induced VEGFR2 Activity	61:88	VEGF-Induced VEGFR2 Activity	61:88	Elements of the Endomucin Extracellular Domain Essential for VEGF-Induced VEGFR2 Activity.					
32517158	7	78	from	function	1342:1349	arg1	internalization					1361:1375	VEGFR2 internalization	1354:1375	VEGFR2 internalization	1354:1375	Mutation of the two N-glycosylation sites on ∆21-121 EMCN abolished its interaction with VEGFR2 and its function in VEGFR2 internalization.					
32517158	3	79	theme	VEGF-induced	496:507	arg1	functions					521:529	VEGF-induced endothelial functions	496:529	VEGF-induced endothelial functions	496:529	To explore the structural components of EMCN that are necessary for its function and the molecular mechanism of EMCN in VEGF-induced endothelial functions, we generated a series of mouse EMCN truncation mutants and examined their ability to rescue VEGF-induced endothelial functions in human primary endothelial cells (EC) in which endogenous EMCN had been knocked down using siRNA.					
32517158	7	80	with	function	1342:1349	arg1	VEGFR2					1327:1332	VEGFR2	1327:1332	VEGFR2	1327:1332	Mutation of the two N-glycosylation sites on ∆21-121 EMCN abolished its interaction with VEGFR2 and its function in VEGFR2 internalization.					
34687022	0	0	theme	Status	79:84	arg1	Nexus					22:26	The Rapidly Expanding Nexus	0:26	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine	0:108	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine.					
34687022	1	1	theme	fragment	242:249	arg1	domain					266:271	the fragment crystallisable domain	238:271	the fragment crystallisable domain	238:271	Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.					
34687022	0	2	theme	Health	72:77	arg1	Status					79:84	Suboptimal Health Status	61:84	Suboptimal Health Status	61:84	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine.					
34687022	1	3	theme	crystallisable	251:264	arg1	domain					266:271	the fragment crystallisable domain	238:271	the fragment crystallisable domain	238:271	Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.					
34687022	5	4	dep	precise	945:951	arg1	dynamic					953:959	dynamic	953:959	dynamic	953:959	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	0	5	theme	Medicine	101:108	arg1	Nexus					22:26	The Rapidly Expanding Nexus	0:26	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine	0:108	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine.					
34687022	3	6	theme	health	537:542	arg1	status					544:549	health status	537:549	health status	537:549	Thus, the immunoglobulin G N-glycome may provide an indication of health status on the spectrum from health to disease and infirmary.					
34687022	1	7	theme	immune	174:179	arg1	responses					181:189	downstream immune responses	163:189	downstream immune responses	163:189	Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.					
34687022	2	8	theme	environmental	403:415	arg1	exposure					417:424	environmental exposure	403:424	environmental exposure	403:424	Collectively termed the N-glycome, it is considered a complex intermediate phenotype: an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course.					
34687022	0	9	theme	Precision	91:99	arg1	Medicine					101:108	Precision Medicine	91:108	Precision Medicine	91:108	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine.					
34687022	3	10	theme	status	544:549	arg1	indication					523:532	an indication	520:532	an indication of health status	520:549	Thus, the immunoglobulin G N-glycome may provide an indication of health status on the spectrum from health to disease and infirmary.					
34687022	1	11	theme	downstream	163:172	arg1	responses					181:189	downstream immune responses	163:189	downstream immune responses	163:189	Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.					
34687022	5	12	theme	biomarkers	961:970	arg1	development					930:940	the development	926:940	the development of precise dynamic biomarkers	926:970	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	2	13	theme	predisposition	387:400	arg1	amalgamation					363:374	an amalgamation	360:374	an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course	360:468	Collectively termed the N-glycome, it is considered a complex intermediate phenotype: an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course.					
34687022	4	14	theme	time	748:751	arg1	periods					737:743	short periods	731:743	short periods of time	731:751	Although variability exists within and between populations, composition of the immunoglobulin G N-glycome remains stable over short periods of time.					
34687022	2	15	theme	genetic	379:385	arg1	predisposition					387:400	genetic predisposition	379:400	genetic predisposition	379:400	Collectively termed the N-glycome, it is considered a complex intermediate phenotype: an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course.					
34687022	5	16	theme	ideal	836:840	arg1	N-glycome					820:828	the immunoglobulin G N-glycome	799:828	the immunoglobulin G N-glycome	799:828	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	5	16	theme	ideal	836:840	arg1	tool					842:845	an ideal tool	833:845	an ideal tool for preclinical disease risk prediction, stratification, and prognosis	833:916	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	2	17	theme	health	431:436	arg1	behaviours					438:447	health behaviours	431:447	health behaviours	431:447	Collectively termed the N-glycome, it is considered a complex intermediate phenotype: an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course.					
34687022	2	18	theme	behaviours	438:447	arg1	amalgamation					363:374	an amalgamation	360:374	an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course	360:468	Collectively termed the N-glycome, it is considered a complex intermediate phenotype: an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course.					
34687022	5	19	theme	precise	945:951	arg1	biomarkers					961:970	precise dynamic biomarkers	945:970	precise dynamic biomarkers	945:970	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	5	20	theme	preclinical	851:861	arg1	prediction					876:885	preclinical disease risk prediction	851:885	preclinical disease risk prediction	851:885	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	0	21	theme	Immunoglobulin	31:44	arg1	N-Glycomics					48:58	Immunoglobulin G N-Glycomics	31:58	Immunoglobulin G N-Glycomics	31:58	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine.					
34687022	2	22	theme	intermediate	336:347	arg1	phenotype					349:357	a complex intermediate phenotype	326:357	a complex intermediate phenotype	326:357	Collectively termed the N-glycome, it is considered a complex intermediate phenotype: an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course.					
34687022	5	23	theme	disease	863:869	arg1	prediction					876:885	preclinical disease risk prediction	851:885	preclinical disease risk prediction	851:885	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	5	24	theme	immunoglobulin	803:816	arg1	N-glycome					820:828	the immunoglobulin G N-glycome	799:828	the immunoglobulin G N-glycome	799:828	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	5	24	theme	immunoglobulin	803:816	arg1	tool					842:845	an ideal tool	833:845	an ideal tool for preclinical disease risk prediction, stratification, and prognosis	833:916	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	2	25	theme	exposure	417:424	arg1	amalgamation					363:374	an amalgamation	360:374	an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course	360:468	Collectively termed the N-glycome, it is considered a complex intermediate phenotype: an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course.					
34687022	0	26	theme	N-Glycomics	48:58	arg1	Nexus					22:26	The Rapidly Expanding Nexus	0:26	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine	0:108	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine.					
34687022	4	27	theme	immunoglobulin	684:697	arg1	N-glycome					701:709	the immunoglobulin G N-glycome	680:709	the immunoglobulin G N-glycome	680:709	Although variability exists within and between populations, composition of the immunoglobulin G N-glycome remains stable over short periods of time.					
34687022	4	28	theme	short	731:735	arg1	periods					737:743	short periods	731:743	short periods of time	731:751	Although variability exists within and between populations, composition of the immunoglobulin G N-glycome remains stable over short periods of time.					
34687022	0	29	theme	G	46:46	arg1	N-Glycomics					48:58	Immunoglobulin G N-Glycomics	31:58	Immunoglobulin G N-Glycomics	31:58	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine.					
34687022	1	30	theme	Immunoglobulin	111:124	arg1	G					126:126	Immunoglobulin G	111:126	Immunoglobulin G	111:126	Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.					
34687022	1	30	theme	Immunoglobulin	111:124	arg1	glycoprotein					143:154	a prevalent glycoprotein	131:154	a prevalent glycoprotein	131:154	Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.					
34687022	4	31	theme	G	699:699	arg1	N-glycome					701:709	the immunoglobulin G N-glycome	680:709	the immunoglobulin G N-glycome	680:709	Although variability exists within and between populations, composition of the immunoglobulin G N-glycome remains stable over short periods of time.					
34687022	2	32	theme	complex	328:334	arg1	phenotype					349:357	a complex intermediate phenotype	326:357	a complex intermediate phenotype	326:357	Collectively termed the N-glycome, it is considered a complex intermediate phenotype: an amalgamation of genetic predisposition, environmental exposure, and health behaviours over the life-course.					
34687022	1	33	gly	glycoprotein	143:154	arg1	G					126:126	Immunoglobulin G	111:126	Immunoglobulin G	111:126	Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.					
34687022	1	33	gly	glycoprotein	143:154	arg1	glycoprotein					143:154	a prevalent glycoprotein	131:154	a prevalent glycoprotein	131:154	Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.					
34687022	3	34	theme	G	496:496	arg1	N-glycome					498:506	the immunoglobulin G N-glycome	477:506	the immunoglobulin G N-glycome	477:506	Thus, the immunoglobulin G N-glycome may provide an indication of health status on the spectrum from health to disease and infirmary.					
34687022	3	35	from	health	572:577	arg1	spectrum					558:565	the spectrum	554:565	the spectrum from health to disease and infirmary	554:602	Thus, the immunoglobulin G N-glycome may provide an indication of health status on the spectrum from health to disease and infirmary.					
34687022	3	36	theme	immunoglobulin	481:494	arg1	N-glycome					498:506	the immunoglobulin G N-glycome	477:506	the immunoglobulin G N-glycome	477:506	Thus, the immunoglobulin G N-glycome may provide an indication of health status on the spectrum from health to disease and infirmary.					
34687022	0	37	theme	Suboptimal	61:70	arg1	Status					79:84	Suboptimal Health Status	61:84	Suboptimal Health Status	61:84	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine.					
34687022	5	38	theme	risk	871:874	arg1	prediction					876:885	preclinical disease risk prediction	851:885	preclinical disease risk prediction	851:885	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	4	39	theme	N-glycome	701:709	arg1	composition					665:675	composition	665:675	composition of the immunoglobulin G N-glycome	665:709	Although variability exists within and between populations, composition of the immunoglobulin G N-glycome remains stable over short periods of time.					
34687022	5	40	theme	G	818:818	arg1	N-glycome					820:828	the immunoglobulin G N-glycome	799:828	the immunoglobulin G N-glycome	799:828	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	5	40	theme	G	818:818	arg1	tool					842:845	an ideal tool	833:845	an ideal tool for preclinical disease risk prediction, stratification, and prognosis	833:916	This underscores the potential of harnessing the immunoglobulin G N-glycome as an ideal tool for preclinical disease risk prediction, stratification, and prognosis through the development of precise dynamic biomarkers.					
34687022	0	41	theme	Expanding	12:20	arg1	Nexus					22:26	The Rapidly Expanding Nexus	0:26	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine	0:108	The Rapidly Expanding Nexus of Immunoglobulin G N-Glycomics, Suboptimal Health Status, and Precision Medicine.					
34687022	1	42	theme	prevalent	133:141	arg1	G					126:126	Immunoglobulin G	111:126	Immunoglobulin G	111:126	Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.					
34687022	1	42	theme	prevalent	133:141	arg1	glycoprotein					143:154	a prevalent glycoprotein	131:154	a prevalent glycoprotein	131:154	Immunoglobulin G is a prevalent glycoprotein, whose downstream immune responses are partially mediated by the N-glycans within the fragment crystallisable domain.					
32747250	5	0	theme	structural	815:824	arg1	diversity					826:834	structural diversity	815:834	structural diversity	815:834	The results provided strong evidence for structural diversity, complexity, and heterogeneity among polysaccharides.					
32747250	8	1	theme	intact	1240:1245	arg1	polysaccharides					1247:1261	intact polysaccharides	1240:1261	intact polysaccharides	1240:1261	The work demonstrated that SEC-SID-MS is a feasible alternative for obtaining valuable structural information from the analysis of intact polysaccharides.					
32747250	2	2	used	used	418:421	arg2	SEC					344:346	SEC	344:346	SEC	344:346	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	2	used	used	418:421	arg2	chromatography					328:341	coupling size exclusion chromatography	304:341	coupling size exclusion chromatography (SEC)	304:347	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	2	used	used	418:421	arg2	MS					369:370	high-resolution MS	353:370	high-resolution MS with source-induced dissociation (SID)	353:409	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	1	3	theme	isomeric	196:203	arg1	heterogeneity					205:217	isomeric heterogeneity	196:217	isomeric heterogeneity	196:217	Characterizing polysaccharides with large molecular weights and isomeric heterogeneity with mass spectrometry (MS) is generally difficult.					
32747250	2	4	theme	source-induced	377:390	arg1	dissociation					392:403	source-induced dissociation	377:403	source-induced dissociation (SID)	377:409	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	4	theme	source-induced	377:390	arg1	SID					406:408	SID	406:408	SID	406:408	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	5	with	chromatography	328:341	arg1	dissociation					392:403	source-induced dissociation	377:403	source-induced dissociation (SID)	377:409	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	5	with	chromatography	328:341	arg1	SID					406:408	SID	406:408	SID	406:408	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	6	theme	exclusion	318:326	arg1	SEC					344:346	SEC	344:346	SEC	344:346	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	6	theme	exclusion	318:326	arg1	chromatography					328:341	coupling size exclusion chromatography	304:341	coupling size exclusion chromatography (SEC)	304:347	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	0	7	theme	plant	109:113	arg1	polysaccharides					115:129	plant polysaccharides	109:129	plant polysaccharides	109:129	Combining online size exclusion chromatography and electrospray ionization mass spectrometry to characterize plant polysaccharides.					
32747250	7	8	theme	Putative	1027:1034	arg1	compositions					1036:1047	Putative compositions	1027:1047	Putative compositions for the fragments	1027:1065	Putative compositions for the fragments were proposed based on exact mass values.					
32747250	2	9	theme	size	313:316	arg1	SEC					344:346	SEC	344:346	SEC	344:346	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	9	theme	size	313:316	arg1	chromatography					328:341	coupling size exclusion chromatography	304:341	coupling size exclusion chromatography (SEC)	304:347	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	6	10	theme	eluted	964:969	arg1	fractions					971:979	eluted fractions	964:979	eluted fractions	964:979	MS showed superior sensitivity and reliability for the polysaccharides in eluted fractions when compared to a refractive index detector.					
32747250	2	11	theme	coupling	304:311	arg1	SEC					344:346	SEC	344:346	SEC	344:346	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	11	theme	coupling	304:311	arg1	chromatography					328:341	coupling size exclusion chromatography	304:341	coupling size exclusion chromatography (SEC)	304:347	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	4	12	theme	numerous	693:700	arg1	fragments					717:725	numerous polysaccharide fragments	693:725	numerous polysaccharide fragments	693:725	This method was used to separate naturally occurring plant polysaccharides based on size, after which numerous polysaccharide fragments were identified from the resulting MS spectra.					
32747250	2	13	theme	intact	478:483	arg1	polysaccharides					485:499	intact polysaccharides	478:499	intact polysaccharides	478:499	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	4	14	theme	occurring	634:642	arg1	polysaccharides					650:664	naturally occurring plant polysaccharides	624:664	naturally occurring plant polysaccharides based on size	624:678	This method was used to separate naturally occurring plant polysaccharides based on size, after which numerous polysaccharide fragments were identified from the resulting MS spectra.					
32747250	1	15	theme	mass	224:227	arg1	spectrometry					229:240	mass spectrometry	224:240	mass spectrometry (MS)	224:245	Characterizing polysaccharides with large molecular weights and isomeric heterogeneity with mass spectrometry (MS) is generally difficult.					
32747250	1	15	theme	mass	224:227	arg1	MS					243:244	MS	243:244	MS	243:244	Characterizing polysaccharides with large molecular weights and isomeric heterogeneity with mass spectrometry (MS) is generally difficult.					
32747250	0	16	theme	online	10:15	arg1	chromatography					32:45	online size exclusion chromatography	10:45	online size exclusion chromatography	10:45	Combining online size exclusion chromatography and electrospray ionization mass spectrometry to characterize plant polysaccharides.					
32747250	1	17	with	polysaccharides	147:161	arg1	weights					184:190	large molecular weights	168:190	large molecular weights	168:190	Characterizing polysaccharides with large molecular weights and isomeric heterogeneity with mass spectrometry (MS) is generally difficult.					
32747250	1	17	with	polysaccharides	147:161	arg1	heterogeneity					205:217	isomeric heterogeneity	196:217	isomeric heterogeneity	196:217	Characterizing polysaccharides with large molecular weights and isomeric heterogeneity with mass spectrometry (MS) is generally difficult.					
32747250	8	18	theme	valuable	1187:1194	arg1	information					1207:1217	valuable structural information	1187:1217	valuable structural information	1187:1217	The work demonstrated that SEC-SID-MS is a feasible alternative for obtaining valuable structural information from the analysis of intact polysaccharides.					
32747250	4	19	theme	polysaccharide	702:715	arg1	fragments					717:725	numerous polysaccharide fragments	693:725	numerous polysaccharide fragments	693:725	This method was used to separate naturally occurring plant polysaccharides based on size, after which numerous polysaccharide fragments were identified from the resulting MS spectra.					
32747250	2	20	theme	structural	453:462	arg1	evaluation					464:473	direct structural evaluation	446:473	direct structural evaluation	446:473	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	21	theme	direct	446:451	arg1	evaluation					464:473	direct structural evaluation	446:473	direct structural evaluation	446:473	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	8	22	theme	polysaccharides	1247:1261	arg1	analysis					1228:1235	the analysis	1224:1235	the analysis of intact polysaccharides	1224:1261	The work demonstrated that SEC-SID-MS is a feasible alternative for obtaining valuable structural information from the analysis of intact polysaccharides.					
32747250	8	23	theme	feasible	1152:1159	arg1	SEC-SID-MS					1136:1145	SEC-SID-MS	1136:1145	SEC-SID-MS	1136:1145	The work demonstrated that SEC-SID-MS is a feasible alternative for obtaining valuable structural information from the analysis of intact polysaccharides.					
32747250	8	23	theme	feasible	1152:1159	arg1	alternative					1161:1171	a feasible alternative	1150:1171	a feasible alternative for obtaining valuable structural information from the analysis of intact polysaccharides	1150:1261	The work demonstrated that SEC-SID-MS is a feasible alternative for obtaining valuable structural information from the analysis of intact polysaccharides.					
32747250	3	24	theme	dextran	557:563	arg1	standards					565:573	dextran standards	557:573	dextran standards	557:573	The analytical method was successfully developed using dextran standards up to 3755 kDa.					
32747250	0	25	theme	exclusion	22:30	arg1	chromatography					32:45	online size exclusion chromatography	10:45	online size exclusion chromatography	10:45	Combining online size exclusion chromatography and electrospray ionization mass spectrometry to characterize plant polysaccharides.					
32747250	7	26	theme	mass	1096:1099	arg1	values					1101:1106	exact mass values	1090:1106	exact mass values	1090:1106	Putative compositions for the fragments were proposed based on exact mass values.					
32747250	5	27	theme	strong	795:800	arg1	evidence					802:809	strong evidence	795:809	strong evidence for structural diversity, complexity, and heterogeneity among polysaccharides	795:887	The results provided strong evidence for structural diversity, complexity, and heterogeneity among polysaccharides.					
32747250	2	28	theme	polysaccharides	485:499	arg1	evaluation					464:473	direct structural evaluation	446:473	direct structural evaluation	446:473	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	28	theme	polysaccharides	485:499	arg1	separation					431:440	separation	431:440	separation	431:440	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	8	29	theme	structural	1196:1205	arg1	information					1207:1217	valuable structural information	1187:1217	valuable structural information	1187:1217	The work demonstrated that SEC-SID-MS is a feasible alternative for obtaining valuable structural information from the analysis of intact polysaccharides.					
32747250	0	30	theme	mass	75:78	arg1	spectrometry					80:91	electrospray ionization mass spectrometry	51:91	electrospray ionization mass spectrometry	51:91	Combining online size exclusion chromatography and electrospray ionization mass spectrometry to characterize plant polysaccharides.					
32747250	4	31	theme	MS	762:763	arg1	spectra					765:771	the resulting MS spectra	748:771	the resulting MS spectra	748:771	This method was used to separate naturally occurring plant polysaccharides based on size, after which numerous polysaccharide fragments were identified from the resulting MS spectra.					
32747250	0	32	theme	electrospray	51:62	arg1	spectrometry					80:91	electrospray ionization mass spectrometry	51:91	electrospray ionization mass spectrometry	51:91	Combining online size exclusion chromatography and electrospray ionization mass spectrometry to characterize plant polysaccharides.					
32747250	6	33	theme	index	1011:1015	arg1	detector					1017:1024	a refractive index detector	998:1024	a refractive index detector	998:1024	MS showed superior sensitivity and reliability for the polysaccharides in eluted fractions when compared to a refractive index detector.					
32747250	3	34	theme	analytical	506:515	arg1	method					517:522	The analytical method	502:522	The analytical method	502:522	The analytical method was successfully developed using dextran standards up to 3755 kDa.					
32747250	2	35	dep	separation	431:440	arg1	the					427:429	the	427:429	the	427:429	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	4	36	theme	resulting	752:760	arg1	spectra					765:771	the resulting MS spectra	748:771	the resulting MS spectra	748:771	This method was used to separate naturally occurring plant polysaccharides based on size, after which numerous polysaccharide fragments were identified from the resulting MS spectra.					
32747250	6	37	theme	refractive	1000:1009	arg1	detector					1017:1024	a refractive index detector	998:1024	a refractive index detector	998:1024	MS showed superior sensitivity and reliability for the polysaccharides in eluted fractions when compared to a refractive index detector.					
32747250	4	38	used	used	607:610	arg2	method					596:601	This method	591:601	This method	591:601	This method was used to separate naturally occurring plant polysaccharides based on size, after which numerous polysaccharide fragments were identified from the resulting MS spectra.					
32747250	6	39	theme	superior	900:907	arg1	sensitivity					909:919	superior sensitivity	900:919	superior sensitivity	900:919	MS showed superior sensitivity and reliability for the polysaccharides in eluted fractions when compared to a refractive index detector.					
32747250	7	40	theme	exact	1090:1094	arg1	values					1101:1106	exact mass values	1090:1106	exact mass values	1090:1106	Putative compositions for the fragments were proposed based on exact mass values.					
32747250	1	41	theme	large	168:172	arg1	weights					184:190	large molecular weights	168:190	large molecular weights	168:190	Characterizing polysaccharides with large molecular weights and isomeric heterogeneity with mass spectrometry (MS) is generally difficult.					
32747250	4	42	theme	plant	644:648	arg1	polysaccharides					650:664	naturally occurring plant polysaccharides	624:664	naturally occurring plant polysaccharides based on size	624:678	This method was used to separate naturally occurring plant polysaccharides based on size, after which numerous polysaccharide fragments were identified from the resulting MS spectra.					
32747250	1	43	theme	molecular	174:182	arg1	weights					184:190	large molecular weights	168:190	large molecular weights	168:190	Characterizing polysaccharides with large molecular weights and isomeric heterogeneity with mass spectrometry (MS) is generally difficult.					
32747250	2	44	theme	high-resolution	353:367	arg1	MS					369:370	high-resolution MS	353:370	high-resolution MS with source-induced dissociation (SID)	353:409	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	45	with	MS	369:370	arg1	dissociation					392:403	source-induced dissociation	377:403	source-induced dissociation (SID)	377:409	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	2	45	with	MS	369:370	arg1	SID					406:408	SID	406:408	SID	406:408	In this work, we demonstrate how coupling size exclusion chromatography (SEC) and high-resolution MS with source-induced dissociation (SID) can be used for the separation and direct structural evaluation of intact polysaccharides.					
32747250	0	46	theme	ionization	64:73	arg1	spectrometry					80:91	electrospray ionization mass spectrometry	51:91	electrospray ionization mass spectrometry	51:91	Combining online size exclusion chromatography and electrospray ionization mass spectrometry to characterize plant polysaccharides.					
32747250	0	47	theme	size	17:20	arg1	chromatography					32:45	online size exclusion chromatography	10:45	online size exclusion chromatography	10:45	Combining online size exclusion chromatography and electrospray ionization mass spectrometry to characterize plant polysaccharides.					
32747250	6	48	from	polysaccharides	945:959	arg1	fractions					971:979	eluted fractions	964:979	eluted fractions	964:979	MS showed superior sensitivity and reliability for the polysaccharides in eluted fractions when compared to a refractive index detector.					
32858448	7	0	theme	structure	1251:1259	arg1	type					1215:1218	type	1215:1218	type of hydrogen bond and secondary structure of protein	1215:1270	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	1	1	from	interactions	188:199	arg1	interface					208:216	the interface	204:216	the interface of biocomposite to increase their applicability	204:264	The main attention of present work is to study the molecular level interactions in the interface of biocomposite to increase their applicability.					
32858448	3	2	theme	in-situ	541:547	arg1	method					566:571	in-situ co-precipitation method	541:571	in-situ co-precipitation method	541:571	In this study, we construct a tri-component system based on silk fibroin/sodium alginate/hydroxyapatite by varying protein and polysaccharide proportions using in-situ co-precipitation method.					
32858448	6	3	theme	HAP	1050:1052	arg1	morphology					1036:1045	morphology	1036:1045	morphology	1036:1045	The crystallographic profile and morphology of HAP were greatly influenced by virtue of polymer matrix.					
32858448	6	3	theme	HAP	1050:1052	arg1	profile					1024:1030	crystallographic profile	1007:1030	crystallographic profile	1007:1030	The crystallographic profile and morphology of HAP were greatly influenced by virtue of polymer matrix.					
32858448	0	4	theme	tri-component	99:111	arg1	system					113:118	tri-component system	99:118	tri-component system	99:118	Investigation on hydrogen bonds and conformational changes in protein/polysaccharide/ceramic based tri-component system.					
32858448	1	5	theme	molecular	172:180	arg1	interactions					188:199	the molecular level interactions	168:199	the molecular level interactions in the interface of biocomposite to increase their applicability	168:264	The main attention of present work is to study the molecular level interactions in the interface of biocomposite to increase their applicability.					
32858448	0	6	from	Investigation	0:12	arg1	protein/polysaccharide/ceramic					62:91	protein/polysaccharide/ceramic	62:91	protein/polysaccharide/ceramic	62:91	Investigation on hydrogen bonds and conformational changes in protein/polysaccharide/ceramic based tri-component system.					
32858448	0	6	from	Investigation	0:12	arg1	bonds					26:30	hydrogen bonds	17:30	hydrogen bonds	17:30	Investigation on hydrogen bonds and conformational changes in protein/polysaccharide/ceramic based tri-component system.					
32858448	2	7	theme	hydrogen	316:323	arg1	bonds					325:329	hydrogen bonds	316:329	hydrogen bonds	316:329	A specific kind of molecular interaction namely, hydrogen bonds play a vital role in deciding composite property.					
32858448	7	8	theme	secondary	1241:1249	arg1	structure					1251:1259	secondary structure	1241:1259	secondary structure of protein	1241:1270	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	4	9	theme	hydrogen	713:720	arg1	bonds					722:726	inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	660:726	inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	660:726	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	2	10	theme	vital	338:342	arg1	role					344:347	a vital role	336:347	a vital role	336:347	A specific kind of molecular interaction namely, hydrogen bonds play a vital role in deciding composite property.					
32858448	4	11	theme	molecular	703:711	arg1	bonds					722:726	inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	660:726	inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	660:726	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	0	12	from	changes	51:57	arg1	protein/polysaccharide/ceramic					62:91	protein/polysaccharide/ceramic	62:91	protein/polysaccharide/ceramic	62:91	Investigation on hydrogen bonds and conformational changes in protein/polysaccharide/ceramic based tri-component system.					
32858448	0	12	from	changes	51:57	arg1	bonds					26:30	hydrogen bonds	17:30	hydrogen bonds	17:30	Investigation on hydrogen bonds and conformational changes in protein/polysaccharide/ceramic based tri-component system.					
32858448	5	13	theme	random	865:870	arg1	coil					872:875	the random coil	861:875	the random coil to β-sheet structure through intermediate β-turns	861:925	During composite preparation, conformational changes from the random coil to β-sheet structure through intermediate β-turns exist within the protein molecule that is confirmed by vibrational spectra.					
32858448	2	14	theme	interaction	296:306	arg1	kind					278:281	A specific kind	267:281	A specific kind of molecular interaction namely	267:313	A specific kind of molecular interaction namely, hydrogen bonds play a vital role in deciding composite property.					
32858448	1	15	theme	level	182:186	arg1	interactions					188:199	the molecular level interactions	168:199	the molecular level interactions in the interface of biocomposite to increase their applicability	168:264	The main attention of present work is to study the molecular level interactions in the interface of biocomposite to increase their applicability.					
32858448	4	16	theme	prepared	633:640	arg1	composite					642:650	prepared composite	633:650	prepared composite	633:650	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	3	17	theme	tri-component	411:423	arg1	system					425:430	a tri-component system	409:430	a tri-component system based on silk fibroin/sodium alginate/hydroxyapatite	409:483	In this study, we construct a tri-component system based on silk fibroin/sodium alginate/hydroxyapatite by varying protein and polysaccharide proportions using in-situ co-precipitation method.					
32858448	7	18	theme	fluid	1122:1126	arg1	study					1144:1148	Simulated body fluid (SBF) immersion study	1107:1148	Simulated body fluid (SBF) immersion study	1107:1148	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	2	19	theme	molecular	286:294	arg1	interaction					296:306	molecular interaction	286:306	molecular interaction namely	286:313	A specific kind of molecular interaction namely, hydrogen bonds play a vital role in deciding composite property.					
32858448	3	20	theme	fibroin/sodium	446:459	arg1	alginate/hydroxyapatite					461:483	silk fibroin/sodium alginate/hydroxyapatite	441:483	silk fibroin/sodium alginate/hydroxyapatite	441:483	In this study, we construct a tri-component system based on silk fibroin/sodium alginate/hydroxyapatite by varying protein and polysaccharide proportions using in-situ co-precipitation method.					
32858448	5	21	theme	conformational	833:846	arg1	changes					848:854	conformational changes	833:854	conformational changes from the random coil to β-sheet structure through intermediate β-turns	833:925	During composite preparation, conformational changes from the random coil to β-sheet structure through intermediate β-turns exist within the protein molecule that is confirmed by vibrational spectra.					
32858448	4	22	theme	FTIR	605:608	arg1	state					622:626	The Fourier Transfer Infrared (FTIR) prediction state	574:626	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	574:726	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	4	22	theme	FTIR	605:608	arg1	varied					751:756	varied	751:756	varied	751:756	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	5	23	theme	β-sheet	880:886	arg1	structure					888:896	β-sheet structure	880:896	β-sheet structure	880:896	During composite preparation, conformational changes from the random coil to β-sheet structure through intermediate β-turns exist within the protein molecule that is confirmed by vibrational spectra.					
32858448	6	24	theme	polymer	1091:1097	arg1	matrix					1099:1104	polymer matrix	1091:1104	polymer matrix	1091:1104	The crystallographic profile and morphology of HAP were greatly influenced by virtue of polymer matrix.					
32858448	4	25	theme	intra-	689:694	arg1	bonds					722:726	inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	660:726	inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	660:726	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	6	26	theme	crystallographic	1007:1022	arg1	profile					1024:1030	crystallographic profile	1007:1030	crystallographic profile	1007:1030	The crystallographic profile and morphology of HAP were greatly influenced by virtue of polymer matrix.					
32858448	7	27	theme	body	1117:1120	arg1	SBF					1129:1131	SBF	1129:1131	SBF	1129:1131	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	7	27	theme	body	1117:1120	arg1	fluid					1122:1126	body fluid	1117:1126	Simulated body fluid (SBF) immersion study	1107:1148	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	1	28	theme	main	125:128	arg1	attention					130:138	The main attention	121:138	The main attention of present work	121:154	The main attention of present work is to study the molecular level interactions in the interface of biocomposite to increase their applicability.					
32858448	6	29	theme	matrix	1099:1104	arg1	virtue					1081:1086	virtue	1081:1086	virtue of polymer matrix	1081:1104	The crystallographic profile and morphology of HAP were greatly influenced by virtue of polymer matrix.					
32858448	0	30	theme	hydrogen	17:24	arg1	bonds					26:30	hydrogen bonds	17:30	hydrogen bonds	17:30	Investigation on hydrogen bonds and conformational changes in protein/polysaccharide/ceramic based tri-component system.					
32858448	4	31	theme	prediction	611:620	arg1	state					622:626	The Fourier Transfer Infrared (FTIR) prediction state	574:626	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	574:726	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	4	31	theme	prediction	611:620	arg1	varied					751:756	varied	751:756	varied	751:756	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	4	32	theme	composite	792:800	arg1	composition					777:787	composition	777:787	composition of composite	777:800	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	2	33	theme	composite	361:369	arg1	property					371:378	composite property	361:378	composite property	361:378	A specific kind of molecular interaction namely, hydrogen bonds play a vital role in deciding composite property.					
32858448	3	34	theme	protein	496:502	arg1	proportions					523:533	protein and polysaccharide proportions	496:533	protein and polysaccharide proportions using in-situ co-precipitation method	496:571	In this study, we construct a tri-component system based on silk fibroin/sodium alginate/hydroxyapatite by varying protein and polysaccharide proportions using in-situ co-precipitation method.					
32858448	0	35	theme	conformational	36:49	arg1	changes					51:57	conformational changes	36:57	conformational changes in protein/polysaccharide/ceramic	36:91	Investigation on hydrogen bonds and conformational changes in protein/polysaccharide/ceramic based tri-component system.					
32858448	8	36	theme	antibacterial	1332:1344	arg1	activity					1346:1353	antibacterial activity	1332:1353	antibacterial activity	1332:1353	Moreover, the in-vitro biomineralization, cytotoxicity and antibacterial activity of composite were analysed in detail.					
32858448	5	37	theme	intermediate	906:917	arg1	β-turns					919:925	intermediate β-turns	906:925	intermediate β-turns	906:925	During composite preparation, conformational changes from the random coil to β-sheet structure through intermediate β-turns exist within the protein molecule that is confirmed by vibrational spectra.					
32858448	1	38	theme	biocomposite	221:232	arg1	interface					208:216	the interface	204:216	the interface of biocomposite to increase their applicability	204:264	The main attention of present work is to study the molecular level interactions in the interface of biocomposite to increase their applicability.					
32858448	3	39	theme	silk	441:444	arg1	alginate/hydroxyapatite					461:483	silk fibroin/sodium alginate/hydroxyapatite	441:483	silk fibroin/sodium alginate/hydroxyapatite	441:483	In this study, we construct a tri-component system based on silk fibroin/sodium alginate/hydroxyapatite by varying protein and polysaccharide proportions using in-situ co-precipitation method.					
32858448	4	40	theme	Fourier	578:584	arg1	state					622:626	The Fourier Transfer Infrared (FTIR) prediction state	574:626	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	574:726	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	4	40	theme	Fourier	578:584	arg1	varied					751:756	varied	751:756	varied	751:756	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	7	41	theme	Simulated	1107:1115	arg1	study					1144:1148	Simulated body fluid (SBF) immersion study	1107:1148	Simulated body fluid (SBF) immersion study	1107:1148	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	5	42	from	coil	872:875	arg1	changes					848:854	conformational changes	833:854	conformational changes from the random coil to β-sheet structure through intermediate β-turns	833:925	During composite preparation, conformational changes from the random coil to β-sheet structure through intermediate β-turns exist within the protein molecule that is confirmed by vibrational spectra.					
32858448	7	43	theme	bond	1232:1235	arg1	type					1215:1218	type	1215:1218	type of hydrogen bond and secondary structure of protein	1215:1270	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	5	44	theme	composite	810:818	arg1	preparation					820:830	composite preparation	810:830	composite preparation	810:830	During composite preparation, conformational changes from the random coil to β-sheet structure through intermediate β-turns exist within the protein molecule that is confirmed by vibrational spectra.					
32858448	5	45	theme	protein	944:950	arg1	molecule					952:959	the protein molecule	940:959	the protein molecule that is confirmed by vibrational spectra	940:1000	During composite preparation, conformational changes from the random coil to β-sheet structure through intermediate β-turns exist within the protein molecule that is confirmed by vibrational spectra.					
32858448	7	46	theme	swelling	1180:1187	arg1	ratio					1189:1193	swelling ratio	1180:1193	swelling ratio	1180:1193	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	4	47	theme	Infrared	595:602	arg1	state					622:626	The Fourier Transfer Infrared (FTIR) prediction state	574:626	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	574:726	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	4	47	theme	Infrared	595:602	arg1	varied					751:756	varied	751:756	varied	751:756	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	7	48	theme	immersion	1134:1142	arg1	study					1144:1148	Simulated body fluid (SBF) immersion study	1107:1148	Simulated body fluid (SBF) immersion study	1107:1148	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	3	49	theme	co-precipitation	549:564	arg1	method					566:571	in-situ co-precipitation method	541:571	in-situ co-precipitation method	541:571	In this study, we construct a tri-component system based on silk fibroin/sodium alginate/hydroxyapatite by varying protein and polysaccharide proportions using in-situ co-precipitation method.					
32858448	1	50	theme	present	143:149	arg1	work					151:154	present work	143:154	present work	143:154	The main attention of present work is to study the molecular level interactions in the interface of biocomposite to increase their applicability.					
32858448	4	51	theme	Transfer	586:593	arg1	state					622:626	The Fourier Transfer Infrared (FTIR) prediction state	574:626	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	574:726	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	4	51	theme	Transfer	586:593	arg1	varied					751:756	varied	751:756	varied	751:756	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	1	52	theme	work	151:154	arg1	attention					130:138	The main attention	121:138	The main attention of present work	121:154	The main attention of present work is to study the molecular level interactions in the interface of biocomposite to increase their applicability.					
32858448	5	53	theme	vibrational	982:992	arg1	spectra					994:1000	vibrational spectra	982:1000	vibrational spectra	982:1000	During composite preparation, conformational changes from the random coil to β-sheet structure through intermediate β-turns exist within the protein molecule that is confirmed by vibrational spectra.					
32858448	4	54	theme	inter-	660:665	arg1	bonds					722:726	inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	660:726	inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds	660:726	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	8	55	dep	biomineralization	1296:1312	arg1	in-vitro					1287:1294	the in-vitro biomineralization, cytotoxicity and antibacterial activity of composite	1283:1366	the in-vitro biomineralization, cytotoxicity and antibacterial activity of composite	1283:1366	Moreover, the in-vitro biomineralization, cytotoxicity and antibacterial activity of composite were analysed in detail.					
32858448	4	56	dep	inter-	660:665	arg1	OH⋯OH					696:700	OH⋯OH	696:700	OH⋯OH	696:700	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	4	56	dep	inter-	660:665	arg1	OH⋯π					679:682	OH⋯π	679:682	OH⋯π	679:682	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	4	56	dep	inter-	660:665	arg1	OH⋯O					673:676	OH⋯O	673:676	OH⋯O	673:676	The Fourier Transfer Infrared (FTIR) prediction state that prepared composite exhibit inter-(OH⋯N, OH⋯O, OH⋯π) and intra-(OH⋯OH) molecular hydrogen bonds and their strength are varied in accordance with composition of composite.					
32858448	3	57	theme	polysaccharide	508:521	arg1	proportions					523:533	protein and polysaccharide proportions	496:533	protein and polysaccharide proportions using in-situ co-precipitation method	496:571	In this study, we construct a tri-component system based on silk fibroin/sodium alginate/hydroxyapatite by varying protein and polysaccharide proportions using in-situ co-precipitation method.					
32858448	8	58	theme	composite	1358:1366	arg1	cytotoxicity					1315:1326	cytotoxicity	1315:1326	cytotoxicity	1315:1326	Moreover, the in-vitro biomineralization, cytotoxicity and antibacterial activity of composite were analysed in detail.					
32858448	8	58	theme	composite	1358:1366	arg1	activity					1346:1353	antibacterial activity	1332:1353	antibacterial activity	1332:1353	Moreover, the in-vitro biomineralization, cytotoxicity and antibacterial activity of composite were analysed in detail.					
32858448	8	58	theme	composite	1358:1366	arg1	biomineralization					1296:1312	biomineralization	1296:1312	biomineralization	1296:1312	Moreover, the in-vitro biomineralization, cytotoxicity and antibacterial activity of composite were analysed in detail.					
32858448	7	59	theme	protein	1264:1270	arg1	bond					1232:1235	hydrogen bond	1223:1235	hydrogen bond	1223:1235	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	7	59	theme	protein	1264:1270	arg1	structure					1251:1259	secondary structure	1241:1259	secondary structure of protein	1241:1270	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32858448	2	60	theme	specific	269:276	arg1	kind					278:281	A specific kind	267:281	A specific kind of molecular interaction namely	267:313	A specific kind of molecular interaction namely, hydrogen bonds play a vital role in deciding composite property.					
32858448	7	61	theme	hydrogen	1223:1230	arg1	bond					1232:1235	hydrogen bond	1223:1235	hydrogen bond	1223:1235	Simulated body fluid (SBF) immersion study shows that biodegradation and swelling ratio are correlated with type of hydrogen bond and secondary structure of protein.					
32241441	5	0	theme	extended	766:773	arg1	release					775:781	an extended release	763:781	an extended release of amoxicillin over 60 min	763:808	These capsules exhibited an extended release of amoxicillin over 60 min in simulated gastric fluid.					
32241441	6	1	theme	similar	916:922	arg1	properties					924:933	similar properties	916:933	similar properties	916:933	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	6	2	theme	Mw	1082:1083	arg1	pullulan					1085:1092	high Mw pullulan	1077:1092	high Mw pullulan	1077:1092	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	3	3	theme	optimal	497:503	arg1	solutions					505:513	the optimal solutions	493:513	the optimal solutions for capsule production	493:536	This high Mw pullulan and commercial low Mw pullulan were respectively co-blended with gellan to prepare the optimal solutions for capsule production.					
32241441	4	4	theme	low	674:676	arg1	leakage					706:712	leakage	706:712	leakage	706:712	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	4	4	theme	low	674:676	arg1	brittleness					693:703	brittleness	693:703	brittleness	693:703	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	4	4	theme	low	674:676	arg1	content					684:690	low water content	674:690	low water content	674:690	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	1	5	theme	non-gelatin	246:256	arg1	capsules					258:265	non-gelatin capsules	246:265	non-gelatin capsules in healthcare aspects	246:287	Albeit gelatin hard capsules were predominantly applied, their disadvantages call for endeavours on non-gelatin capsules in healthcare aspects.					
32241441	6	6	theme	composite	892:900	arg1	capsules					902:909	composite capsules	892:909	composite capsules with similar properties	892:933	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	7	7	with	substitution	1126:1137	arg1	composites					1171:1180	pullulan-gellan composites	1155:1180	pullulan-gellan composites for preparing hard capsules	1155:1208	This work proposes a potential substitution of gelatin with pullulan-gellan composites for preparing hard capsules.					
32241441	3	8	theme	capsule	519:525	arg1	production					527:536	capsule production	519:536	capsule production	519:536	This high Mw pullulan and commercial low Mw pullulan were respectively co-blended with gellan to prepare the optimal solutions for capsule production.					
32241441	2	9	theme	fermentation	368:379	arg1	broth					381:385	yeast fermentation broth	362:385	yeast fermentation broth	362:385	Herein, high molecular weight (Mw = 1 × 106) pullulan was purified from yeast fermentation broth.					
32241441	3	10	theme	low	425:427	arg1	Mw					429:430	commercial low Mw	414:430	commercial low Mw	414:430	This high Mw pullulan and commercial low Mw pullulan were respectively co-blended with gellan to prepare the optimal solutions for capsule production.					
32241441	7	11	theme	hard	1196:1199	arg1	capsules					1201:1208	hard capsules	1196:1208	hard capsules	1196:1208	This work proposes a potential substitution of gelatin with pullulan-gellan composites for preparing hard capsules.					
32241441	6	12	theme	pullulan	874:881	arg1	use					851:853	the use	847:853	the use of high and low Mw pullulan	847:881	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	1	13	theme	gelatin	153:159	arg1	capsules					166:173	gelatin hard capsules	153:173	gelatin hard capsules	153:173	Albeit gelatin hard capsules were predominantly applied, their disadvantages call for endeavours on non-gelatin capsules in healthcare aspects.					
32241441	0	14	theme	Alternative	3:13	arg1	Capsule					20:26	An Alternative Hard Capsule	0:26	An Alternative Hard Capsule	0:26	An Alternative Hard Capsule Prepared with the High Molecular Weight Pullulan and Gellan: Processing, Characterization, and In Vitro Drug Release.					
32241441	6	15	theme	Mw	871:872	arg1	pullulan					874:881	high and low Mw pullulan	858:881	high and low Mw pullulan	858:881	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	2	16	theme	molecular	303:311	arg1	Mw = 1 × 106					321:332	Mw = 1 × 106	321:332	Mw = 1 × 106	321:332	Herein, high molecular weight (Mw = 1 × 106) pullulan was purified from yeast fermentation broth.					
32241441	2	16	theme	molecular	303:311	arg1	weight					313:318	high molecular weight	298:318	high molecular weight (Mw = 1 × 106) pullulan	298:342	Herein, high molecular weight (Mw = 1 × 106) pullulan was purified from yeast fermentation broth.					
32241441	1	17	theme	hard	161:164	arg1	capsules					166:173	gelatin hard capsules	153:173	gelatin hard capsules	153:173	Albeit gelatin hard capsules were predominantly applied, their disadvantages call for endeavours on non-gelatin capsules in healthcare aspects.					
32241441	5	18	theme	simulated	813:821	arg1	fluid					831:835	simulated gastric fluid	813:835	simulated gastric fluid	813:835	These capsules exhibited an extended release of amoxicillin over 60 min in simulated gastric fluid.					
32241441	6	19	theme	pullulan	974:981	arg1	half					994:997	nearly half	987:997	nearly half of that for low Mw pullulan	987:1025	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	6	19	theme	pullulan	974:981	arg1	concentration					949:961	the required concentration	936:961	the required concentration of high Mw pullulan	936:981	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	2	20	theme	high	298:301	arg1	Mw = 1 × 106					321:332	Mw = 1 × 106	321:332	Mw = 1 × 106	321:332	Herein, high molecular weight (Mw = 1 × 106) pullulan was purified from yeast fermentation broth.					
32241441	2	20	theme	high	298:301	arg1	weight					313:318	high molecular weight	298:318	high molecular weight (Mw = 1 × 106) pullulan	298:342	Herein, high molecular weight (Mw = 1 × 106) pullulan was purified from yeast fermentation broth.					
32241441	2	21	theme	yeast	362:366	arg1	broth					381:385	yeast fermentation broth	362:385	yeast fermentation broth	362:385	Herein, high molecular weight (Mw = 1 × 106) pullulan was purified from yeast fermentation broth.					
32241441	5	22	theme	gastric	823:829	arg1	fluid					831:835	simulated gastric fluid	813:835	simulated gastric fluid	813:835	These capsules exhibited an extended release of amoxicillin over 60 min in simulated gastric fluid.					
32241441	1	23	theme	healthcare	270:279	arg1	aspects					281:287	healthcare aspects	270:287	healthcare aspects	270:287	Albeit gelatin hard capsules were predominantly applied, their disadvantages call for endeavours on non-gelatin capsules in healthcare aspects.					
32241441	0	24	dep	Weight	61:66	arg1	Pullulan					68:75	Pullulan	68:75	Pullulan	68:75	An Alternative Hard Capsule Prepared with the High Molecular Weight Pullulan and Gellan: Processing, Characterization, and In Vitro Drug Release.					
32241441	0	24	dep	Weight	61:66	arg1	Gellan					81:86	Gellan	81:86	Gellan	81:86	An Alternative Hard Capsule Prepared with the High Molecular Weight Pullulan and Gellan: Processing, Characterization, and In Vitro Drug Release.					
32241441	4	25	theme	pullulan-gellan	641:655	arg1	capsules					657:664	the obtained pullulan-gellan capsules	628:664	the obtained pullulan-gellan capsules	628:664	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	0	26	theme	Hard	15:18	arg1	Capsule					20:26	An Alternative Hard Capsule	0:26	An Alternative Hard Capsule	0:26	An Alternative Hard Capsule Prepared with the High Molecular Weight Pullulan and Gellan: Processing, Characterization, and In Vitro Drug Release.					
32241441	6	27	theme	high	966:969	arg1	pullulan					974:981	high Mw pullulan	966:981	high Mw pullulan	966:981	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	1	28	from	capsules	258:265	arg1	aspects					281:287	healthcare aspects	270:287	healthcare aspects	270:287	Albeit gelatin hard capsules were predominantly applied, their disadvantages call for endeavours on non-gelatin capsules in healthcare aspects.					
32241441	6	29	theme	low	867:869	arg1	pullulan					874:881	high and low Mw pullulan	858:881	high and low Mw pullulan	858:881	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	6	30	theme	high	1077:1080	arg1	pullulan					1085:1092	high Mw pullulan	1077:1092	high Mw pullulan	1077:1092	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	7	31	theme	gelatin	1142:1148	arg1	substitution					1126:1137	a potential substitution	1114:1137	a potential substitution of gelatin with pullulan-gellan composites for preparing hard capsules	1114:1208	This work proposes a potential substitution of gelatin with pullulan-gellan composites for preparing hard capsules.					
32241441	4	32	theme	obtained	632:639	arg1	capsules					657:664	the obtained pullulan-gellan capsules	628:664	the obtained pullulan-gellan capsules	628:664	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	3	33	theme	Mw	398:399	arg1	pullulan					401:408	high Mw pullulan	393:408	high Mw pullulan	393:408	This high Mw pullulan and commercial low Mw pullulan were respectively co-blended with gellan to prepare the optimal solutions for capsule production.					
32241441	6	34	with	capsules	902:909	arg1	properties					924:933	similar properties	916:933	similar properties	916:933	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	0	35	theme	In	123:124	arg1	Release					137:143	In Vitro Drug Release	123:143	In Vitro Drug Release	123:143	An Alternative Hard Capsule Prepared with the High Molecular Weight Pullulan and Gellan: Processing, Characterization, and In Vitro Drug Release.					
32241441	3	36	theme	commercial	414:423	arg1	Mw					429:430	commercial low Mw	414:430	commercial low Mw	414:430	This high Mw pullulan and commercial low Mw pullulan were respectively co-blended with gellan to prepare the optimal solutions for capsule production.					
32241441	7	37	theme	potential	1116:1124	arg1	substitution					1126:1137	a potential substitution	1114:1137	a potential substitution of gelatin with pullulan-gellan composites for preparing hard capsules	1114:1208	This work proposes a potential substitution of gelatin with pullulan-gellan composites for preparing hard capsules.					
32241441	3	38	theme	pullulan	401:408	arg1	pullulan					432:439	This high Mw pullulan and commercial low Mw pullulan	388:439	This high Mw pullulan and commercial low Mw pullulan	388:439	This high Mw pullulan and commercial low Mw pullulan were respectively co-blended with gellan to prepare the optimal solutions for capsule production.					
32241441	5	39	theme	amoxicillin	786:796	arg1	release					775:781	an extended release	763:781	an extended release of amoxicillin over 60 min	763:808	These capsules exhibited an extended release of amoxicillin over 60 min in simulated gastric fluid.					
32241441	6	40	theme	high	858:861	arg1	pullulan					874:881	high and low Mw pullulan	858:881	high and low Mw pullulan	858:881	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	0	41	theme	High	46:49	arg1	Weight					61:66	the High Molecular Weight	42:66	the High Molecular Weight Pullulan and Gellan	42:86	An Alternative Hard Capsule Prepared with the High Molecular Weight Pullulan and Gellan: Processing, Characterization, and In Vitro Drug Release.					
32241441	6	42	theme	required	940:947	arg1	half					994:997	nearly half	987:997	nearly half of that for low Mw pullulan	987:1025	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	6	42	theme	required	940:947	arg1	concentration					949:961	the required concentration	936:961	the required concentration of high Mw pullulan	936:981	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	0	43	theme	Drug	132:135	arg1	Release					137:143	In Vitro Drug Release	123:143	In Vitro Drug Release	123:143	An Alternative Hard Capsule Prepared with the High Molecular Weight Pullulan and Gellan: Processing, Characterization, and In Vitro Drug Release.					
32241441	6	44	theme	Mw	971:972	arg1	pullulan					974:981	high Mw pullulan	966:981	high Mw pullulan	966:981	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	3	45	theme	high	393:396	arg1	pullulan					401:408	high Mw pullulan	393:408	high Mw pullulan	393:408	This high Mw pullulan and commercial low Mw pullulan were respectively co-blended with gellan to prepare the optimal solutions for capsule production.					
32241441	4	46	theme	Further	539:545	arg1	tightness					605:613	tightness	605:613	tightness	605:613	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	4	46	theme	Further	539:545	arg1	brittleness					589:599	brittleness	589:599	brittleness	589:599	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	4	46	theme	Further	539:545	arg1	investigations					547:560	Further investigations	539:560	Further investigations	539:560	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	4	46	theme	Further	539:545	arg1	loss-on-drying					573:586	loss-on-drying	573:586	loss-on-drying	573:586	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	4	47	theme	water	678:682	arg1	leakage					706:712	leakage	706:712	leakage	706:712	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	4	47	theme	water	678:682	arg1	brittleness					693:703	brittleness	693:703	brittleness	693:703	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	4	47	theme	water	678:682	arg1	content					684:690	low water content	674:690	low water content	674:690	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	4	48	theme	high	722:725	arg1	tightness					727:735	high tightness	722:735	high tightness	722:735	Further investigations, including loss-on-drying, brittleness and tightness, showed that the obtained pullulan-gellan capsules were of low water content, brittleness, leakage, and of high tightness.					
32241441	6	49	theme	Mw	1015:1016	arg1	pullulan					1018:1025	low Mw pullulan	1011:1025	low Mw pullulan	1011:1025	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	6	50	theme	cost-saving	1041:1051	arg1	characteristic					1053:1066	a cost-saving characteristic	1039:1066	a cost-saving characteristic of using high Mw pullulan	1039:1092	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	2	51	theme	weight	313:318	arg1	pullulan					335:342	high molecular weight (Mw = 1 × 106) pullulan	298:342	high molecular weight (Mw = 1 × 106) pullulan	298:342	Herein, high molecular weight (Mw = 1 × 106) pullulan was purified from yeast fermentation broth.					
32241441	6	52	theme	low	1011:1013	arg1	pullulan					1018:1025	low Mw pullulan	1011:1025	low Mw pullulan	1011:1025	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	0	53	dep	In	123:124	arg1	Vitro					126:130	Vitro	126:130	Vitro	126:130	An Alternative Hard Capsule Prepared with the High Molecular Weight Pullulan and Gellan: Processing, Characterization, and In Vitro Drug Release.					
32241441	3	54	theme	Mw	429:430	arg1	pullulan					432:439	This high Mw pullulan and commercial low Mw pullulan	388:439	This high Mw pullulan and commercial low Mw pullulan	388:439	This high Mw pullulan and commercial low Mw pullulan were respectively co-blended with gellan to prepare the optimal solutions for capsule production.					
32241441	0	55	theme	Molecular	51:59	arg1	Weight					61:66	the High Molecular Weight	42:66	the High Molecular Weight Pullulan and Gellan	42:86	An Alternative Hard Capsule Prepared with the High Molecular Weight Pullulan and Gellan: Processing, Characterization, and In Vitro Drug Release.					
32241441	7	56	theme	pullulan-gellan	1155:1169	arg1	composites					1171:1180	pullulan-gellan composites	1155:1180	pullulan-gellan composites for preparing hard capsules	1155:1208	This work proposes a potential substitution of gelatin with pullulan-gellan composites for preparing hard capsules.					
32241441	6	57	theme	that	1002:1005	arg1	half					994:997	nearly half	987:997	nearly half of that for low Mw pullulan	987:1025	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
32241441	6	57	theme	that	1002:1005	arg1	concentration					949:961	the required concentration	936:961	the required concentration of high Mw pullulan	936:981	Although the use of high and low Mw pullulan produced composite capsules with similar properties, the required concentration of high Mw pullulan was nearly half of that for low Mw pullulan, suggesting a cost-saving characteristic of using high Mw pullulan.					
33578865	0	0	theme	Polysaccharide	57:70	arg1	Composition					72:82	Microalgae Polysaccharide Composition	46:82	Microalgae Polysaccharide Composition	46:82	A New, Quick, and Simple Protocol to Evaluate Microalgae Polysaccharide Composition.					
33578865	5	1	theme	TFA	1139:1141	arg1	presence					1127:1134	the presence	1123:1134	the presence of TFA and NaOH	1123:1150	Initial results showed that the enzymes were not sensitive to the presence of TFA and NaOH, so the methodology could be carried out on fresh hydrolysate.					
33578865	6	2	theme	quantification	1229:1242	arg1	limits					1219:1224	The limits	1215:1224	The limits of quantification of the method	1215:1256	The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance.					
33578865	6	3	theme	chromatographic	1378:1392	arg1	methods					1394:1400	the chromatographic methods	1374:1400	the chromatographic methods	1374:1400	The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance.					
33578865	5	4	theme	NaOH	1147:1150	arg1	presence					1127:1134	the presence	1123:1134	the presence of TFA and NaOH	1123:1150	Initial results showed that the enzymes were not sensitive to the presence of TFA and NaOH, so the methodology could be carried out on fresh hydrolysate.					
33578865	0	5	theme	Microalgae	46:55	arg1	Composition					72:82	Microalgae Polysaccharide Composition	46:82	Microalgae Polysaccharide Composition	46:82	A New, Quick, and Simple Protocol to Evaluate Microalgae Polysaccharide Composition.					
33578865	1	6	theme	algal	302:306	arg1	cultures					308:315	algal cultures	302:315	algal cultures	302:315	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	4	7	dep	carried	884:890	arg1	out					892:894	out	892:894	out	892:894	Quantification was then carried out directly on the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis.					
33578865	5	8	theme	fresh	1196:1200	arg1	hydrolysate					1202:1212	fresh hydrolysate	1196:1212	fresh hydrolysate	1196:1212	Initial results showed that the enzymes were not sensitive to the presence of TFA and NaOH, so the methodology could be carried out on fresh hydrolysate.					
33578865	3	9	theme	2	795:795	arg1	M					797:797	M	797:797	M	797:797	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	8	10	theme	miliQ	1756:1760	arg1	water					1762:1766	miliQ water	1756:1766	miliQ water	1756:1766	Finally, analyses of raw culture media were carried out and compared to the results obtained in miliQ water; no differences were observed.					
33578865	9	11	theme	quality	1890:1896	arg1	monitoring					1872:1881	routine monitoring	1864:1881	routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors	1864:1969	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	6	12	theme	analytical	1414:1423	arg1	performance					1425:1435	analytical performance	1414:1435	analytical performance	1414:1435	The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance.					
33578865	7	13	theme	protocol	1650:1657	arg1	closeness					1633:1641	the closeness	1629:1641	the closeness of the protocol	1629:1657	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	3	14	theme	acid	778:781	arg1	acid					815:818	2 M trifluoroacetic acid	795:818	2 M trifluoroacetic acid	795:818	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	3	14	theme	acid	778:781	arg1	hydrolysis					783:792	acid hydrolysis	778:792	acid hydrolysis (2 M trifluoroacetic acid)	778:819	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	2	15	theme	chromatographic	582:596	arg1	methods					598:604	chromatographic methods	582:604	chromatographic methods	582:604	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	8	16	theme	raw	1681:1683	arg1	media					1693:1697	raw culture media	1681:1697	raw culture media	1681:1697	Finally, analyses of raw culture media were carried out and compared to the results obtained in miliQ water; no differences were observed.					
33578865	7	17	theme	enzymatic	1492:1500	arg1	method					1502:1507	the enzymatic method	1488:1507	the enzymatic method	1488:1507	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	3	18	theme	hydrolysis	783:792	arg1	application					763:773	the application	759:773	the application of acid hydrolysis (2 M trifluoroacetic acid)	759:819	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	2	19	theme	pharmaceutical	458:471	arg1	activities					473:482	exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities	414:482	exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods	414:604	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	5	20	theme	Initial	1061:1067	arg1	results					1069:1075	Initial results	1061:1075	Initial results	1061:1075	Initial results showed that the enzymes were not sensitive to the presence of TFA and NaOH, so the methodology could be carried out on fresh hydrolysate.					
33578865	2	21	theme	target	360:365	arg1	oligomers					367:375	target oligomers	360:375	target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system	360:703	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	9	22	theme	quick	1821:1825	arg1	method					1848:1853	a quick, functional analysis method	1819:1853	a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors	1819:1969	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	9	22	theme	quick	1821:1825	arg1	approach					1807:1814	The new approach	1799:1814	The new approach	1799:1814	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	6	23	theme	log	1302:1304	arg1	order					1289:1293	the order	1285:1293	the order of the log of nanograms of monosaccharides	1285:1336	The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance.					
33578865	2	24	dep	rhamnose	491:498	arg1	i.e.					485:488	i.e.	485:488	i.e.	485:488	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	0	25	theme	New	2:4	arg1	Protocol					25:32	A New, Quick, and Simple Protocol	0:32	A New, Quick, and Simple Protocol	0:32	A New, Quick, and Simple Protocol to Evaluate Microalgae Polysaccharide Composition.					
33578865	4	26	theme	fresh	912:916	arg1	hydrolysate					918:928	the fresh hydrolysate	908:928	the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis	908:1058	Quantification was then carried out directly on the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis.					
33578865	2	27	theme	culture	690:696	arg1	system					698:703	the culture system	686:703	the culture system	686:703	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	1	28	theme	high	145:148	arg1	specificity					150:160	the high specificity	141:160	the high specificity of enzymes in a complex mixture	141:192	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	9	29	theme	analysis	1839:1846	arg1	method					1848:1853	a quick, functional analysis method	1819:1853	a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors	1819:1969	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	9	29	theme	analysis	1839:1846	arg1	approach					1807:1814	The new approach	1799:1814	The new approach	1799:1814	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	0	30	theme	Quick	7:11	arg1	Protocol					25:32	A New, Quick, and Simple Protocol	0:32	A New, Quick, and Simple Protocol	0:32	A New, Quick, and Simple Protocol to Evaluate Microalgae Polysaccharide Composition.					
33578865	4	31	from	monosaccharides	974:988	arg1	composition					1010:1020	a pre-determined composition	993:1020	a pre-determined composition of the polysaccharides under analysis	993:1058	Quantification was then carried out directly on the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis.					
33578865	9	32	theme	algal	1930:1934	arg1	cultures					1936:1943	algal cultures	1930:1943	algal cultures grown in photobioreactors	1930:1969	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	2	33	from	monitoring	672:681	arg1	system					698:703	the culture system	686:703	the culture system	686:703	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	9	34	theme	bioactive	1901:1909	arg1	polysaccharides					1911:1925	bioactive polysaccharides	1901:1925	bioactive polysaccharides	1901:1925	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	1	35	theme	bioactive	240:248	arg1	polysaccharides					250:264	bioactive polysaccharides	240:264	bioactive polysaccharides produced by microalgae	240:287	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	3	36	dep	required	750:757	arg1	followed					821:828	followed	821:828	followed by NaOH (2 M) neutralization	821:857	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	9	37	theme	routine	1864:1870	arg1	monitoring					1872:1881	routine monitoring	1864:1881	routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors	1864:1969	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	7	38	theme	recovery	1597:1604	arg1	rates					1606:1610	good recovery rates	1592:1610	good recovery rates	1592:1610	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	1	39	theme	enzymes	165:171	arg1	specificity					150:160	the high specificity	141:160	the high specificity of enzymes in a complex mixture	141:192	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	1	40	theme	polysaccharides	250:264	arg1	composition					225:235	the composition	221:235	the composition of bioactive polysaccharides produced by microalgae	221:287	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	7	41	theme	results	1468:1474	arg1	analysis					1452:1459	A comparative analysis	1438:1459	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography)	1438:1580	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	6	42	theme	performance	1425:1435	arg1	terms					1405:1409	terms	1405:1409	terms of analytical performance	1405:1435	The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance.					
33578865	9	43	from	monitoring	1872:1881	arg1	cultures					1936:1943	algal cultures	1930:1943	algal cultures grown in photobioreactors	1930:1969	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	4	44	theme	enzyme	936:941	arg1	kits					943:946	enzyme kits	936:946	enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis	936:1058	Quantification was then carried out directly on the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis.					
33578865	3	45	theme	monosaccharide	727:740	arg1	content					742:748	the monosaccharide content	723:748	the monosaccharide content	723:748	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	2	46	dep	activities	473:482	arg1	rhamnose					491:498	rhamnose	491:498	rhamnose	491:498	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	2	46	dep	activities	473:482	arg1	etc.					524:527	etc.	524:527	etc.	524:527	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	2	46	dep	activities	473:482	arg1	fucose					501:506	fucose	501:506	fucose	501:506	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	2	46	dep	activities	473:482	arg1	sugars					516:521	acidic sugars	509:521	acidic sugars	509:521	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	4	47	theme	pre-determined	995:1008	arg1	composition					1010:1020	a pre-determined composition	993:1020	a pre-determined composition of the polysaccharides under analysis	993:1058	Quantification was then carried out directly on the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis.					
33578865	8	48	theme	media	1693:1697	arg1	analyses					1669:1676	analyses	1669:1676	analyses of raw culture media	1669:1697	Finally, analyses of raw culture media were carried out and compared to the results obtained in miliQ water; no differences were observed.					
33578865	6	49	theme	monosaccharides	1322:1336	arg1	nanograms					1309:1317	nanograms	1309:1317	nanograms of monosaccharides	1309:1336	The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance.					
33578865	3	50	theme	content	742:748	arg1	Determination					706:718	Determination	706:718	Determination of the monosaccharide content	706:748	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	6	51	theme	method	1251:1256	arg1	quantification					1229:1242	quantification	1229:1242	quantification of the method	1229:1256	The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance.					
33578865	7	52	theme	good	1592:1595	arg1	rates					1606:1610	good recovery rates	1592:1610	good recovery rates	1592:1610	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	8	53	theme	culture	1685:1691	arg1	media					1693:1697	raw culture media	1681:1697	raw culture media	1681:1697	Finally, analyses of raw culture media were carried out and compared to the results obtained in miliQ water; no differences were observed.					
33578865	0	54	theme	Simple	18:23	arg1	Protocol					25:32	A New, Quick, and Simple Protocol	0:32	A New, Quick, and Simple Protocol	0:32	A New, Quick, and Simple Protocol to Evaluate Microalgae Polysaccharide Composition.					
33578865	9	55	theme	new	1803:1805	arg1	method					1848:1853	a quick, functional analysis method	1819:1853	a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors	1819:1969	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	9	55	theme	new	1803:1805	arg1	approach					1807:1814	The new approach	1799:1814	The new approach	1799:1814	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	2	56	theme	acidic	509:514	arg1	rhamnose					491:498	rhamnose	491:498	rhamnose	491:498	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	2	56	theme	acidic	509:514	arg1	sugars					516:521	acidic sugars	509:521	acidic sugars	509:521	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	2	57	theme	high	651:654	arg1	resolution					627:636	a resolution	625:636	a resolution sufficiently high	625:654	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	4	58	theme	polysaccharides	1029:1043	arg1	composition					1010:1020	a pre-determined composition	993:1020	a pre-determined composition of the polysaccharides under analysis	993:1058	Quantification was then carried out directly on the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis.					
33578865	2	59	dep	nutraceutical	440:452	arg1	exopolysaccharides					414:431	exopolysaccharides'	414:432	exopolysaccharides'	414:432	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	7	60	theme	comparative	1440:1450	arg1	analysis					1452:1459	A comparative analysis	1438:1459	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography)	1438:1580	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	1	61	from	enzymes	165:171	arg1	mixture					186:192	a complex mixture	176:192	a complex mixture	176:192	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	7	62	theme	high-performance	1534:1549	arg1	method					1526:1531	a reference method	1514:1531	a reference method (high-performance anion-exchange chromatography)	1514:1580	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	7	62	theme	high-performance	1534:1549	arg1	chromatography					1566:1579	high-performance anion-exchange chromatography	1534:1579	high-performance anion-exchange chromatography	1534:1579	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	1	63	theme	complex	178:184	arg1	mixture					186:192	a complex mixture	176:192	a complex mixture	176:192	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	1	64	from	mixture	186:192	arg1	specificity					150:160	the high specificity	141:160	the high specificity of enzymes in a complex mixture	141:192	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	6	65	theme	nanograms	1309:1317	arg1	log					1302:1304	the log	1298:1304	the log of nanograms of monosaccharides	1298:1336	The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance.					
33578865	1	66	theme	new	101:103	arg1	approach					120:127	a new methodological approach	99:127	a new methodological approach	99:127	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	3	67	theme	M	797:797	arg1	acid					815:818	2 M trifluoroacetic acid	795:818	2 M trifluoroacetic acid	795:818	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	3	67	theme	M	797:797	arg1	hydrolysis					783:792	acid hydrolysis	778:792	acid hydrolysis (2 M trifluoroacetic acid)	778:819	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	7	68	theme	reference	1516:1524	arg1	method					1526:1531	a reference method	1514:1531	a reference method (high-performance anion-exchange chromatography)	1514:1580	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	7	68	theme	reference	1516:1524	arg1	chromatography					1566:1579	high-performance anion-exchange chromatography	1534:1579	high-performance anion-exchange chromatography	1534:1579	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	2	69	theme	nutraceutical	440:452	arg1	activities					473:482	exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities	414:482	exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods	414:604	The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides' (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system.					
33578865	1	70	from	specificity	150:160	arg1	mixture					186:192	a complex mixture	176:192	a complex mixture	176:192	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	1	71	theme	methodological	105:118	arg1	approach					120:127	a new methodological approach	99:127	a new methodological approach	99:127	In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures.					
33578865	3	72	theme	trifluoroacetic	799:813	arg1	acid					815:818	2 M trifluoroacetic acid	795:818	2 M trifluoroacetic acid	795:818	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	3	72	theme	trifluoroacetic	799:813	arg1	hydrolysis					783:792	acid hydrolysis	778:792	acid hydrolysis (2 M trifluoroacetic acid)	778:819	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	9	73	dep	quick	1821:1825	arg1	functional					1828:1837	functional	1828:1837	functional	1828:1837	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	9	74	theme	polysaccharides	1911:1925	arg1	quality					1890:1896	the quality	1886:1896	the quality of bioactive polysaccharides	1886:1925	The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.					
33578865	4	75	theme	main	969:972	arg1	monosaccharides					974:988	the main monosaccharides	965:988	the main monosaccharides in a pre-determined composition of the polysaccharides under analysis	965:1058	Quantification was then carried out directly on the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis.					
33578865	3	76	theme	NaOH	833:836	arg1	neutralization					844:857	NaOH (2 M) neutralization	833:857	NaOH (2 M) neutralization	833:857	Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization.					
33578865	7	77	theme	anion-exchange	1551:1564	arg1	method					1526:1531	a reference method	1514:1531	a reference method (high-performance anion-exchange chromatography)	1514:1580	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
33578865	7	77	theme	anion-exchange	1551:1564	arg1	chromatography					1566:1579	high-performance anion-exchange chromatography	1534:1579	high-performance anion-exchange chromatography	1534:1579	A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol.					
29532110	6	0	theme	fucosylation	1096:1107	arg1	networks					1109:1116	the glycosylation, sialylation, and fucosylation networks	1060:1116	the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production	1060:1210	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	8	1	from	approaches	1377:1386	arg1	examples					1331:1338	examples	1331:1338	examples of their use in CHO cell engineering approaches to highlight these technologies further	1331:1426	We provide examples of their use in CHO cell engineering approaches to highlight these technologies further.					
29532110	3	2	from	interest	613:620	arg1	industry					625:632	industry	625:632	industry	625:632	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	1	3	theme	hamster	115:121	arg1	CHO					130:132	CHO	130:132	CHO	130:132	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	3	theme	hamster	115:121	arg1	ovary					123:127	Chinese hamster ovary	107:127	Chinese hamster ovary (CHO)	107:133	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	6	4	from	overview	1048:1055	arg1	cells					1131:1135	mammalian cells	1121:1135	mammalian cells	1121:1135	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	6	4	from	overview	1048:1055	arg1	cells					1155:1159	CHO cells	1151:1159	specifically CHO cells	1138:1159	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	8	5	theme	engineering	1365:1375	arg1	approaches					1377:1386	CHO cell engineering approaches	1356:1386	CHO cell engineering approaches	1356:1386	We provide examples of their use in CHO cell engineering approaches to highlight these technologies further.					
29532110	1	6	from	role	213:216	arg1	industry					239:246	the biotechnology industry	221:246	the biotechnology industry	221:246	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	3	7	theme	mammalian	535:543	arg1	cells					545:549	these mammalian cells	529:549	these mammalian cells	529:549	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	6	8	used	used	1184:1187	arg2	cells					1131:1135	mammalian cells	1121:1135	mammalian cells	1121:1135	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	6	8	used	used	1184:1187	arg2	cells					1155:1159	CHO cells	1151:1159	specifically CHO cells	1138:1159	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	1	9	theme	human	161:165	arg1	HEK					185:187	HEK	185:187	HEK	185:187	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	9	theme	human	161:165	arg1	kidney					177:182	human embryonic kidney	161:182	human embryonic kidney (HEK) cells	161:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	10	theme	kidney	177:182	arg1	cells					190:194	human embryonic kidney (HEK) cells	161:194	human embryonic kidney (HEK) cells	161:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	10	11	theme	cell	1658:1661	arg1	glycoengineering					1663:1678	CHO cell glycoengineering	1654:1678	CHO cell glycoengineering	1654:1678	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	10	12	theme	glycosylated	1822:1833	arg1	proteins					1835:1842	the glycosylated proteins	1818:1842	the glycosylated proteins in CHO cells	1818:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	5	13	theme	pathway	911:917	arg1	components					879:888	components	879:888	components of the glycosylation pathway that affect circulatory half-life and antibody-dependent cellular cytotoxicity, respectively	879:1010	In addition, sialylation and fucosylation represent components of the glycosylation pathway that affect circulatory half-life and antibody-dependent cellular cytotoxicity, respectively.					
29532110	9	14	theme	sialidase	1548:1556	arg1	cleavage					1558:1565	sialidase cleavage	1548:1565	sialidase cleavage	1548:1565	Specifically, we describe efforts to overexpress glycosyltransferases and sialyltransfereases, and efforts to decrease sialidase cleavage and fucosylation.					
29532110	2	15	theme	quality	476:482	arg1	component					455:463	a principal component	443:463	a principal component of product quality	443:482	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	10	16	gly	glycosylated	1822:1833	arg1	proteins					1835:1842	the glycosylated proteins	1818:1842	the glycosylated proteins in CHO cells	1818:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	3	17	theme	glycosylation	584:596	arg1	engineering					560:570	the engineering	556:570	the engineering of N-linked glycosylation	556:596	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	7	18	theme	genetic	1219:1225	arg1	technologies					1239:1250	genetic engineering technologies	1219:1250	genetic engineering technologies used in CHO cells to modulate glycosylation pathways	1219:1303	Next, genetic engineering technologies used in CHO cells to modulate glycosylation pathways are described.					
29532110	0	19	from	Glycoengineering	0:15	arg1	Level					63:67	a Cellular Level	52:67	a Cellular Level	52:67	Glycoengineering of Mammalian Expression Systems on a Cellular Level.					
29532110	5	20	theme	antibody-dependent	957:974	arg1	cytotoxicity					985:996	antibody-dependent cellular cytotoxicity	957:996	antibody-dependent cellular cytotoxicity	957:996	In addition, sialylation and fucosylation represent components of the glycosylation pathway that affect circulatory half-life and antibody-dependent cellular cytotoxicity, respectively.					
29532110	1	21	theme	protein	297:303	arg1	therapeutics					305:316	recombinant protein therapeutics	285:316	recombinant protein therapeutics	285:316	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	10	22	from	proteins	1835:1842	arg1	cells					1851:1855	CHO cells	1847:1855	CHO cells	1847:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	8	23	theme	cell	1360:1363	arg1	approaches					1377:1386	CHO cell engineering approaches	1356:1386	CHO cell engineering approaches	1356:1386	We provide examples of their use in CHO cell engineering approaches to highlight these technologies further.					
29532110	1	24	theme	expression	80:89	arg1	host					266:269	the production host	251:269	the production host of choice for recombinant protein therapeutics	251:316	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	24	theme	expression	80:89	arg1	cells					190:194	human embryonic kidney (HEK) cells	161:194	human embryonic kidney (HEK) cells	161:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	24	theme	expression	80:89	arg1	myeloma					142:148	mouse myeloma	136:148	mouse myeloma (NS0)	136:154	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	24	theme	expression	80:89	arg1	ovary					123:127	Chinese hamster ovary	107:127	Chinese hamster ovary (CHO)	107:133	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	24	theme	expression	80:89	arg1	systems					91:97	Mammalian expression systems	70:97	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells	70:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	24	theme	expression	80:89	arg1	role					213:216	a critical role	202:216	a critical role in the biotechnology industry	202:246	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	6	25	theme	sialylation	1079:1089	arg1	networks					1109:1116	the glycosylation, sialylation, and fucosylation networks	1060:1116	the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production	1060:1210	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	2	26	theme	glycan	411:416	arg1	attachments					418:428	glycan attachments	411:428	glycan attachments representing a principal component of product quality	411:482	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	8	27	from	examples	1331:1338	arg1	approaches					1377:1386	CHO cell engineering approaches	1356:1386	CHO cell engineering approaches	1356:1386	We provide examples of their use in CHO cell engineering approaches to highlight these technologies further.					
29532110	6	28	theme	glycosylation	1064:1076	arg1	networks					1109:1116	the glycosylation, sialylation, and fucosylation networks	1060:1116	the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production	1060:1210	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	0	29	theme	Mammalian	20:28	arg1	Systems					41:47	Mammalian Expression Systems	20:47	Mammalian Expression Systems	20:47	Glycoengineering of Mammalian Expression Systems on a Cellular Level.					
29532110	1	30	theme	biotechnology	225:237	arg1	industry					239:246	the biotechnology industry	221:246	the biotechnology industry	221:246	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	31	theme	Chinese	107:113	arg1	CHO					130:132	CHO	130:132	CHO	130:132	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	31	theme	Chinese	107:113	arg1	ovary					123:127	Chinese hamster ovary	107:127	Chinese hamster ovary (CHO)	107:133	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	2	32	theme	principal	445:453	arg1	component					455:463	a principal component	443:463	a principal component of product quality	443:482	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	4	33	theme	biotherapeutics	791:805	arg1	quality					768:774	the product quality	756:774	the product quality of recombinant biotherapeutics in mammalian hosts	756:824	This is because altering the N-glycan composition can change the product quality of recombinant biotherapeutics in mammalian hosts.					
29532110	0	34	theme	Systems	41:47	arg1	Glycoengineering					0:15	Glycoengineering	0:15	Glycoengineering of Mammalian Expression Systems on a Cellular Level	0:67	Glycoengineering of Mammalian Expression Systems on a Cellular Level.					
29532110	4	35	theme	N-glycan	724:731	arg1	composition					733:743	the N-glycan composition	720:743	the N-glycan composition	720:743	This is because altering the N-glycan composition can change the product quality of recombinant biotherapeutics in mammalian hosts.					
29532110	9	36	dep	efforts	1455:1461	arg1	overexpress					1466:1476	overexpress	1466:1476	to overexpress glycosyltransferases and sialyltransfereases	1463:1521	Specifically, we describe efforts to overexpress glycosyltransferases and sialyltransfereases, and efforts to decrease sialidase cleavage and fucosylation.					
29532110	9	36	dep	efforts	1455:1461	arg1	decrease					1539:1546	decrease	1539:1546	to decrease sialidase cleavage and fucosylation	1536:1582	Specifically, we describe efforts to overexpress glycosyltransferases and sialyltransfereases, and efforts to decrease sialidase cleavage and fucosylation.					
29532110	1	37	theme	production	255:264	arg1	host					266:269	the production host	251:269	the production host of choice for recombinant protein therapeutics	251:316	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	37	theme	production	255:264	arg1	cells					190:194	human embryonic kidney (HEK) cells	161:194	human embryonic kidney (HEK) cells	161:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	37	theme	production	255:264	arg1	myeloma					142:148	mouse myeloma	136:148	mouse myeloma (NS0)	136:154	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	37	theme	production	255:264	arg1	ovary					123:127	Chinese hamster ovary	107:127	Chinese hamster ovary (CHO)	107:133	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	37	theme	production	255:264	arg1	systems					91:97	Mammalian expression systems	70:97	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells	70:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	37	theme	production	255:264	arg1	role					213:216	a critical role	202:216	a critical role in the biotechnology industry	202:246	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	4	38	theme	product	760:766	arg1	quality					768:774	the product quality	756:774	the product quality of recombinant biotherapeutics in mammalian hosts	756:824	This is because altering the N-glycan composition can change the product quality of recombinant biotherapeutics in mammalian hosts.					
29532110	6	39	theme	mammalian	1121:1129	arg1	cells					1131:1135	mammalian cells	1121:1135	mammalian cells	1121:1135	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	6	39	theme	mammalian	1121:1129	arg1	cells					1155:1159	CHO cells	1151:1159	specifically CHO cells	1138:1159	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	3	40	theme	many	638:641	arg1	efforts					643:649	many efforts	638:649	many efforts	638:649	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	8	41	from	use	1349:1351	arg1	approaches					1377:1386	CHO cell engineering approaches	1356:1386	CHO cell engineering approaches	1356:1386	We provide examples of their use in CHO cell engineering approaches to highlight these technologies further.					
29532110	6	42	theme	networks	1109:1116	arg1	overview					1048:1055	an overview	1045:1055	an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production	1045:1210	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	6	43	from	networks	1109:1116	arg1	cells					1131:1135	mammalian cells	1121:1135	mammalian cells	1121:1135	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	6	43	from	networks	1109:1116	arg1	cells					1155:1159	CHO cells	1151:1159	specifically CHO cells	1138:1159	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	10	44	theme	omics	1760:1764	arg1	integration					1744:1754	the integration	1740:1754	the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells	1740:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	10	44	theme	omics	1760:1764	arg1	glycomics					1725:1733	glycomics	1725:1733	glycomics	1725:1733	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	10	44	theme	omics	1760:1764	arg1	glycoproteomics					1708:1722	glycoproteomics	1708:1722	glycoproteomics	1708:1722	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	4	45	theme	mammalian	810:818	arg1	hosts					820:824	mammalian hosts	810:824	mammalian hosts	810:824	This is because altering the N-glycan composition can change the product quality of recombinant biotherapeutics in mammalian hosts.					
29532110	1	46	theme	critical	204:211	arg1	host					266:269	the production host	251:269	the production host of choice for recombinant protein therapeutics	251:316	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	46	theme	critical	204:211	arg1	cells					190:194	human embryonic kidney (HEK) cells	161:194	human embryonic kidney (HEK) cells	161:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	46	theme	critical	204:211	arg1	myeloma					142:148	mouse myeloma	136:148	mouse myeloma (NS0)	136:154	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	46	theme	critical	204:211	arg1	ovary					123:127	Chinese hamster ovary	107:127	Chinese hamster ovary (CHO)	107:133	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	46	theme	critical	204:211	arg1	systems					91:97	Mammalian expression systems	70:97	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells	70:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	46	theme	critical	204:211	arg1	role					213:216	a critical role	202:216	a critical role in the biotechnology industry	202:246	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	10	47	theme	new	1614:1616	arg1	application					1693:1703	the application	1689:1703	the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells	1689:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	10	47	theme	new	1614:1616	arg1	strategies					1618:1627	new strategies	1614:1627	new strategies	1614:1627	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	10	48	theme	CHO	1654:1656	arg1	glycoengineering					1663:1678	CHO cell glycoengineering	1654:1678	CHO cell glycoengineering	1654:1678	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	3	49	located	present	518:524	arg2	O-glycans					504:512	O-glycans	504:512	O-glycans	504:512	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	3	49	located	present	518:524	arg2	N-glycans					490:498	N-glycans	490:498	N-glycans	490:498	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	3	49	located	present	518:524	arg1	cells					545:549	these mammalian cells	529:549	these mammalian cells	529:549	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	6	50	theme	CHO	1151:1153	arg1	cells					1131:1135	mammalian cells	1121:1135	mammalian cells	1121:1135	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	6	50	theme	CHO	1151:1153	arg1	cells					1155:1159	CHO cells	1151:1159	specifically CHO cells	1138:1159	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	10	51	theme	future	1633:1638	arg1	application					1693:1703	the application	1689:1703	the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells	1689:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	10	51	theme	future	1633:1638	arg1	directions					1640:1649	future directions	1633:1649	future directions of CHO cell glycoengineering	1633:1678	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	10	52	theme	CHO	1847:1849	arg1	cells					1851:1855	CHO cells	1847:1855	CHO cells	1847:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	1	53	theme	embryonic	167:175	arg1	HEK					185:187	HEK	185:187	HEK	185:187	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	53	theme	embryonic	167:175	arg1	kidney					177:182	human embryonic kidney	161:182	human embryonic kidney (HEK) cells	161:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	10	54	theme	glycoengineering	1663:1678	arg1	application					1693:1703	the application	1689:1703	the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells	1689:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	10	54	theme	glycoengineering	1663:1678	arg1	strategies					1618:1627	new strategies	1614:1627	new strategies	1614:1627	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	10	54	theme	glycoengineering	1663:1678	arg1	directions					1640:1649	future directions	1633:1649	future directions of CHO cell glycoengineering	1633:1678	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	5	55	theme	glycosylation	897:909	arg1	pathway					911:917	the glycosylation pathway	893:917	the glycosylation pathway	893:917	In addition, sialylation and fucosylation represent components of the glycosylation pathway that affect circulatory half-life and antibody-dependent cellular cytotoxicity, respectively.					
29532110	3	56	from	cells	545:549	arg1	present					518:524	present	518:524	present	518:524	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	7	57	theme	engineering	1227:1237	arg1	technologies					1239:1250	genetic engineering technologies	1219:1250	genetic engineering technologies used in CHO cells to modulate glycosylation pathways	1219:1303	Next, genetic engineering technologies used in CHO cells to modulate glycosylation pathways are described.					
29532110	2	58	contain	contain	376:382	arg1	glycoproteins					357:369	glycoproteins	357:369	glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality	357:482	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	2	58	contain	contain	376:382	arg1	biologics					343:351	the recombinant biologics	327:351	the recombinant biologics	327:351	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	2	58	contain	contain	376:382	arg2	attachments					418:428	glycan attachments	411:428	glycan attachments representing a principal component of product quality	411:482	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	2	58	contain	contain	376:382	arg2	oligosaccharide					392:406	complex oligosaccharide	384:406	complex oligosaccharide	384:406	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	2	58	contain	contain	376:382	arg1	Most					319:322	Most	319:322	Most	319:322	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	3	59	theme	N-linked	575:582	arg1	glycosylation					584:596	N-linked glycosylation	575:596	N-linked glycosylation	575:596	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	4	60	from	quality	768:774	arg1	hosts					820:824	mammalian hosts	810:824	mammalian hosts	810:824	This is because altering the N-glycan composition can change the product quality of recombinant biotherapeutics in mammalian hosts.					
29532110	2	61	theme	product	468:474	arg1	quality					476:482	product quality	468:482	product quality	468:482	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	6	62	theme	antibody	1192:1199	arg1	production					1201:1210	antibody production	1192:1210	antibody production	1192:1210	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	1	63	theme	choice	274:279	arg1	host					266:269	the production host	251:269	the production host of choice for recombinant protein therapeutics	251:316	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	63	theme	choice	274:279	arg1	cells					190:194	human embryonic kidney (HEK) cells	161:194	human embryonic kidney (HEK) cells	161:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	63	theme	choice	274:279	arg1	myeloma					142:148	mouse myeloma	136:148	mouse myeloma (NS0)	136:154	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	63	theme	choice	274:279	arg1	ovary					123:127	Chinese hamster ovary	107:127	Chinese hamster ovary (CHO)	107:133	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	63	theme	choice	274:279	arg1	systems					91:97	Mammalian expression systems	70:97	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells	70:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	63	theme	choice	274:279	arg1	role					213:216	a critical role	202:216	a critical role in the biotechnology industry	202:246	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	64	theme	mouse	136:140	arg1	myeloma					142:148	mouse myeloma	136:148	mouse myeloma (NS0)	136:154	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	3	65	from	present	518:524	arg1	cells					545:549	these mammalian cells	529:549	these mammalian cells	529:549	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	1	66	theme	recombinant	285:295	arg1	therapeutics					305:316	recombinant protein therapeutics	285:316	recombinant protein therapeutics	285:316	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	5	67	theme	cellular	976:983	arg1	cytotoxicity					985:996	antibody-dependent cellular cytotoxicity	957:996	antibody-dependent cellular cytotoxicity	957:996	In addition, sialylation and fucosylation represent components of the glycosylation pathway that affect circulatory half-life and antibody-dependent cellular cytotoxicity, respectively.					
29532110	2	68	gly	glycoproteins	357:369	arg1	glycoproteins					357:369	glycoproteins	357:369	glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality	357:482	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	2	68	gly	glycoproteins	357:369	arg1	biologics					343:351	the recombinant biologics	327:351	the recombinant biologics	327:351	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	2	68	gly	glycoproteins	357:369	arg1	Most					319:322	Most	319:322	Most	319:322	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	8	69	theme	use	1349:1351	arg1	examples					1331:1338	examples	1331:1338	examples of their use in CHO cell engineering approaches to highlight these technologies further	1331:1426	We provide examples of their use in CHO cell engineering approaches to highlight these technologies further.					
29532110	1	70	theme	Mammalian	70:78	arg1	host					266:269	the production host	251:269	the production host of choice for recombinant protein therapeutics	251:316	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	70	theme	Mammalian	70:78	arg1	cells					190:194	human embryonic kidney (HEK) cells	161:194	human embryonic kidney (HEK) cells	161:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	70	theme	Mammalian	70:78	arg1	myeloma					142:148	mouse myeloma	136:148	mouse myeloma (NS0)	136:154	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	70	theme	Mammalian	70:78	arg1	ovary					123:127	Chinese hamster ovary	107:127	Chinese hamster ovary (CHO)	107:133	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	70	theme	Mammalian	70:78	arg1	systems					91:97	Mammalian expression systems	70:97	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells	70:194	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	1	70	theme	Mammalian	70:78	arg1	role					213:216	a critical role	202:216	a critical role in the biotechnology industry	202:246	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	8	71	theme	CHO	1356:1358	arg1	approaches					1377:1386	CHO cell engineering approaches	1356:1386	CHO cell engineering approaches	1356:1386	We provide examples of their use in CHO cell engineering approaches to highlight these technologies further.					
29532110	2	72	theme	recombinant	331:341	arg1	biologics					343:351	the recombinant biologics	327:351	the recombinant biologics	327:351	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	11	73	dep	Abstract	1868:1875	arg1	Graphical					1858:1866	Graphical	1858:1866	Graphical	1858:1866	Graphical Abstract.					
29532110	5	74	theme	circulatory	931:941	arg1	half-life					943:951	circulatory half-life	931:951	circulatory half-life	931:951	In addition, sialylation and fucosylation represent components of the glycosylation pathway that affect circulatory half-life and antibody-dependent cellular cytotoxicity, respectively.					
29532110	1	75	dep	myeloma	142:148	arg1	NS0					151:153	NS0	151:153	NS0	151:153	Mammalian expression systems such as Chinese hamster ovary (CHO), mouse myeloma (NS0), and human embryonic kidney (HEK) cells serve a critical role in the biotechnology industry as the production host of choice for recombinant protein therapeutics.					
29532110	0	76	theme	Expression	30:39	arg1	Systems					41:47	Mammalian Expression Systems	20:47	Mammalian Expression Systems	20:47	Glycoengineering of Mammalian Expression Systems on a Cellular Level.					
29532110	3	77	attach	present	518:524	arg2	O-glycans					504:512	O-glycans	504:512	O-glycans	504:512	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	3	77	attach	present	518:524	arg2	N-glycans					490:498	N-glycans	490:498	N-glycans	490:498	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	3	77	attach	present	518:524	arg1	cells					545:549	these mammalian cells	529:549	these mammalian cells	529:549	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	2	78	theme	complex	384:390	arg1	oligosaccharide					392:406	complex oligosaccharide	384:406	complex oligosaccharide	384:406	Most of the recombinant biologics are glycoproteins that contain complex oligosaccharide or glycan attachments representing a principal component of product quality.					
29532110	10	79	theme	glycomics	1725:1733	arg1	application					1693:1703	the application	1689:1703	the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells	1689:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	3	80	theme	critical	604:611	arg1	interest					613:620	critical interest	604:620	critical interest in industry	604:632	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
29532110	4	81	theme	recombinant	779:789	arg1	biotherapeutics					791:805	recombinant biotherapeutics	779:805	recombinant biotherapeutics	779:805	This is because altering the N-glycan composition can change the product quality of recombinant biotherapeutics in mammalian hosts.					
29532110	6	82	from	cells	1131:1135	arg1	overview					1048:1055	an overview	1045:1055	an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production	1045:1210	In this chapter, we first offer an overview of the glycosylation, sialylation, and fucosylation networks in mammalian cells, specifically CHO cells, which are extensively used in antibody production.					
29532110	10	83	theme	glycoproteomics	1708:1722	arg1	application					1693:1703	the application	1689:1703	the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells	1689:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	0	84	theme	Cellular	54:61	arg1	Level					63:67	a Cellular Level	52:67	a Cellular Level	52:67	Glycoengineering of Mammalian Expression Systems on a Cellular Level.					
29532110	7	85	theme	glycosylation	1282:1294	arg1	pathways					1296:1303	glycosylation pathways	1282:1303	glycosylation pathways	1282:1303	Next, genetic engineering technologies used in CHO cells to modulate glycosylation pathways are described.					
29532110	10	86	theme	integration	1744:1754	arg1	application					1693:1703	the application	1689:1703	the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells	1689:1855	Finally, this chapter covers new strategies and future directions of CHO cell glycoengineering, such as the application of glycoproteomics, glycomics, and the integration of 'omics' approaches to identify, quantify, and characterize the glycosylated proteins in CHO cells.					
29532110	7	87	theme	CHO	1260:1262	arg1	cells					1264:1268	CHO cells	1260:1268	CHO cells	1260:1268	Next, genetic engineering technologies used in CHO cells to modulate glycosylation pathways are described.					
29532110	3	88	link	N-linked	575:582	arg1	glycosylation					584:596	N-linked glycosylation	575:596	N-linked glycosylation	575:596	Both N-glycans and O-glycans are present in these mammalian cells, but the engineering of N-linked glycosylation is of critical interest in industry and many efforts have been directed to improve this pathway.					
34048831	0	0	theme	gut	79:81	arg1	microbiota					83:92	human gut microbiota	73:92	human gut microbiota	73:92	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.					
34048831	5	1	theme	fermentation	799:810	arg1	outcomes					812:819	the fermentation outcomes	795:819	the fermentation outcomes of the three fibers	795:839	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	0	2	theme	human	73:77	arg1	microbiota					83:92	human gut microbiota	73:92	human gut microbiota	73:92	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.					
34048831	7	3	theme	personalized	1174:1185	arg1	nutrition					1187:1195	personalized nutrition	1174:1195	personalized nutrition using dietary fibers	1174:1216	Our study demonstrates an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives and highlights that personalized nutrition using dietary fibers should be tailored according to individual's composition of the gut microbiome.					
34048831	6	4	theme	butyrate	1025:1032	arg1	butyrate					1025:1032	butyrate	1025:1032	butyrate	1025:1032	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	6	4	theme	butyrate	1025:1032	arg1	amount					999:1004	the highest amount	987:1004	the highest amount of total SCFAs and butyrate	987:1032	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	6	4	theme	butyrate	1025:1032	arg1	SCFAs					1015:1019	total SCFAs	1009:1019	total SCFAs	1009:1019	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	6	5	theme	alginate	926:933	arg1	Fermentation					910:921	Fermentation	910:921	Fermentation of alginate and PM by Bacteroides-dominated microbiota	910:976	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	0	6	theme	microbiota	83:92	arg1	enterotypes					58:68	different enterotypes	48:68	different enterotypes of human gut microbiota	48:92	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.					
34048831	6	7	theme	SCFAs	1015:1019	arg1	butyrate					1025:1032	butyrate	1025:1032	butyrate	1025:1032	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	6	7	theme	SCFAs	1015:1019	arg1	amount					999:1004	the highest amount	987:1004	the highest amount of total SCFAs and butyrate	987:1032	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	6	7	theme	SCFAs	1015:1019	arg1	SCFAs					1015:1019	total SCFAs	1009:1019	total SCFAs	1009:1019	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	0	8	theme	personalized	103:114	arg1	nutrition					116:124	personalized nutrition	103:124	personalized nutrition using enterotype-specific dietary fibers	103:165	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.					
34048831	5	9	theme	fatty	871:875	arg1	acids					877:881	short-chain fatty acids	859:881	short-chain fatty acids (SCFAs) that are produced	859:907	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	5	9	theme	fatty	871:875	arg1	SCFAs					884:888	SCFAs	884:888	SCFAs	884:888	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	7	10	theme	dietary	1203:1209	arg1	fibers					1211:1216	dietary fibers	1203:1216	dietary fibers	1203:1216	Our study demonstrates an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives and highlights that personalized nutrition using dietary fibers should be tailored according to individual's composition of the gut microbiome.					
34048831	7	11	from	effect	1081:1086	arg1	fermentation					1109:1120	the fermentation	1105:1120	the fermentation of alginate and its derivatives	1105:1152	Our study demonstrates an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives and highlights that personalized nutrition using dietary fibers should be tailored according to individual's composition of the gut microbiome.					
34048831	3	12	theme	microbiota	430:439	arg1	compositions					410:421	different compositions	400:421	different compositions of the microbiota	400:439	However, how different compositions of the microbiota may affect the fermentation outcomes of these polysaccharides remains unknown.					
34048831	6	13	theme	highest	991:997	arg1	butyrate					1025:1032	butyrate	1025:1032	butyrate	1025:1032	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	6	13	theme	highest	991:997	arg1	amount					999:1004	the highest amount	987:1004	the highest amount of total SCFAs and butyrate	987:1032	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	6	13	theme	highest	991:997	arg1	SCFAs					1015:1019	total SCFAs	1009:1019	total SCFAs	1009:1019	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	7	14	theme	alginate	1125:1132	arg1	fermentation					1109:1120	the fermentation	1105:1120	the fermentation of alginate and its derivatives	1105:1152	Our study demonstrates an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives and highlights that personalized nutrition using dietary fibers should be tailored according to individual's composition of the gut microbiome.					
34048831	2	15	theme	polymannuronate	317:331	arg1	PM					339:340	PM	339:340	PM	339:340	Previous studies indicated that alginate, polyguluronate (PG) and polymannuronate acid (PM) could be fermented by human gut microbiota.					
34048831	2	15	theme	polymannuronate	317:331	arg1	acid					333:336	polymannuronate acid	317:336	polymannuronate acid (PM)	317:341	Previous studies indicated that alginate, polyguluronate (PG) and polymannuronate acid (PM) could be fermented by human gut microbiota.					
34048831	2	16	theme	gut	371:373	arg1	microbiota					375:384	human gut microbiota	365:384	human gut microbiota	365:384	Previous studies indicated that alginate, polyguluronate (PG) and polymannuronate acid (PM) could be fermented by human gut microbiota.					
34048831	6	17	theme	total	1009:1013	arg1	SCFAs					1015:1019	total SCFAs	1009:1019	total SCFAs	1009:1019	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	2	18	theme	human	365:369	arg1	microbiota					375:384	human gut microbiota	365:384	human gut microbiota	365:384	Previous studies indicated that alginate, polyguluronate (PG) and polymannuronate acid (PM) could be fermented by human gut microbiota.					
34048831	4	19	dep	PG	642:643	arg1	compared					655:662	compared	655:662	compared to Prevotella-dominated (Prevotella enterotype) and Escherichia-dominated microbiota (Escherichia enterotype)	655:772	Here we show that Bacteroides-dominated microbiota (Bacteroides enterotype) is more proficient at degrading and utilizing PG and PM as compared to Prevotella-dominated (Prevotella enterotype) and Escherichia-dominated microbiota (Escherichia enterotype).					
34048831	4	20	dep	microbiota	738:747	arg1	enterotype					762:771	Escherichia enterotype	750:771	Escherichia enterotype	750:771	Here we show that Bacteroides-dominated microbiota (Bacteroides enterotype) is more proficient at degrading and utilizing PG and PM as compared to Prevotella-dominated (Prevotella enterotype) and Escherichia-dominated microbiota (Escherichia enterotype).					
34048831	7	21	theme	derivatives	1142:1152	arg1	fermentation					1109:1120	the fermentation	1105:1120	the fermentation of alginate and its derivatives	1105:1152	Our study demonstrates an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives and highlights that personalized nutrition using dietary fibers should be tailored according to individual's composition of the gut microbiome.					
34048831	5	22	theme	short-chain	859:869	arg1	acids					877:881	short-chain fatty acids	859:881	short-chain fatty acids (SCFAs) that are produced	859:907	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	5	22	theme	short-chain	859:869	arg1	SCFAs					884:888	SCFAs	884:888	SCFAs	884:888	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	0	23	theme	alginate	16:23	arg1	Fermentation					0:11	Fermentation	0:11	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.	0:166	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.					
34048831	6	24	theme	PM	939:940	arg1	Fermentation					910:921	Fermentation	910:921	Fermentation of alginate and PM by Bacteroides-dominated microbiota	910:976	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	3	25	theme	fermentation	456:467	arg1	outcomes					469:476	the fermentation outcomes	452:476	the fermentation outcomes of these polysaccharides	452:501	However, how different compositions of the microbiota may affect the fermentation outcomes of these polysaccharides remains unknown.					
34048831	0	26	theme	derivatives	33:43	arg1	Fermentation					0:11	Fermentation	0:11	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.	0:166	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.					
34048831	4	27	theme	Bacteroides	572:582	arg1	microbiota					560:569	Bacteroides-dominated microbiota	538:569	Bacteroides-dominated microbiota (Bacteroides enterotype)	538:594	Here we show that Bacteroides-dominated microbiota (Bacteroides enterotype) is more proficient at degrading and utilizing PG and PM as compared to Prevotella-dominated (Prevotella enterotype) and Escherichia-dominated microbiota (Escherichia enterotype).					
34048831	4	27	theme	Bacteroides	572:582	arg1	enterotype					584:593	Bacteroides enterotype	572:593	Bacteroides enterotype	572:593	Here we show that Bacteroides-dominated microbiota (Bacteroides enterotype) is more proficient at degrading and utilizing PG and PM as compared to Prevotella-dominated (Prevotella enterotype) and Escherichia-dominated microbiota (Escherichia enterotype).					
34048831	0	28	theme	dietary	152:158	arg1	fibers					160:165	enterotype-specific dietary fibers	132:165	enterotype-specific dietary fibers	132:165	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.					
34048831	5	29	theme	acids	877:881	arg1	acids					877:881	short-chain fatty acids	859:881	short-chain fatty acids (SCFAs) that are produced	859:907	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	5	29	theme	acids	877:881	arg1	fibers					834:839	the three fibers	824:839	the three fibers	824:839	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	5	29	theme	acids	877:881	arg1	amount					849:854	the amount	845:854	the amount of short-chain fatty acids (SCFAs) that are produced	845:907	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	5	29	theme	acids	877:881	arg1	SCFAs					884:888	SCFAs	884:888	SCFAs	884:888	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	5	29	theme	acids	877:881	arg1	outcomes					812:819	the fermentation outcomes	795:819	the fermentation outcomes of the three fibers	795:839	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	1	30	theme	food	216:219	arg1	Alginate					168:175	Alginate	168:175	Alginate	168:175	Alginate and its derivatives are widely used as food additives and dietary fibers.					
34048831	1	30	theme	food	216:219	arg1	derivatives					185:195	its derivatives	181:195	its derivatives	181:195	Alginate and its derivatives are widely used as food additives and dietary fibers.					
34048831	1	30	theme	food	216:219	arg1	additives					221:229	food additives	216:229	food additives	216:229	Alginate and its derivatives are widely used as food additives and dietary fibers.					
34048831	3	31	theme	different	400:408	arg1	compositions					410:421	different compositions	400:421	different compositions of the microbiota	400:439	However, how different compositions of the microbiota may affect the fermentation outcomes of these polysaccharides remains unknown.					
34048831	0	32	theme	enterotype-specific	132:150	arg1	fibers					160:165	enterotype-specific dietary fibers	132:165	enterotype-specific dietary fibers	132:165	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.					
34048831	0	33	theme	different	48:56	arg1	enterotypes					58:68	different enterotypes	48:68	different enterotypes of human gut microbiota	48:92	Fermentation of alginate and its derivatives by different enterotypes of human gut microbiota: Towards personalized nutrition using enterotype-specific dietary fibers.					
34048831	5	34	theme	fibers	834:839	arg1	acids					877:881	short-chain fatty acids	859:881	short-chain fatty acids (SCFAs) that are produced	859:907	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	5	34	theme	fibers	834:839	arg1	fibers					834:839	the three fibers	824:839	the three fibers	824:839	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	5	34	theme	fibers	834:839	arg1	amount					849:854	the amount	845:854	the amount of short-chain fatty acids (SCFAs) that are produced	845:907	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	5	34	theme	fibers	834:839	arg1	SCFAs					884:888	SCFAs	884:888	SCFAs	884:888	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	5	34	theme	fibers	834:839	arg1	outcomes					812:819	the fermentation outcomes	795:819	the fermentation outcomes of the three fibers	795:839	Enterotype dictates the fermentation outcomes of the three fibers and the amount of short-chain fatty acids (SCFAs) that are produced.					
34048831	4	35	theme	Bacteroides-dominated	538:558	arg1	microbiota					560:569	Bacteroides-dominated microbiota	538:569	Bacteroides-dominated microbiota (Bacteroides enterotype)	538:594	Here we show that Bacteroides-dominated microbiota (Bacteroides enterotype) is more proficient at degrading and utilizing PG and PM as compared to Prevotella-dominated (Prevotella enterotype) and Escherichia-dominated microbiota (Escherichia enterotype).					
34048831	4	35	theme	Bacteroides-dominated	538:558	arg1	enterotype					584:593	Bacteroides enterotype	572:593	Bacteroides enterotype	572:593	Here we show that Bacteroides-dominated microbiota (Bacteroides enterotype) is more proficient at degrading and utilizing PG and PM as compared to Prevotella-dominated (Prevotella enterotype) and Escherichia-dominated microbiota (Escherichia enterotype).					
34048831	7	36	theme	microbiota	1091:1100	arg1	effect					1081:1086	an enterotype-specific effect	1058:1086	an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives	1058:1152	Our study demonstrates an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives and highlights that personalized nutrition using dietary fibers should be tailored according to individual's composition of the gut microbiome.					
34048831	1	37	used	used	208:211	arg2	additives					221:229	food additives	216:229	food additives	216:229	Alginate and its derivatives are widely used as food additives and dietary fibers.					
34048831	1	37	used	used	208:211	arg2	Alginate					168:175	Alginate	168:175	Alginate	168:175	Alginate and its derivatives are widely used as food additives and dietary fibers.					
34048831	1	37	used	used	208:211	arg2	derivatives					185:195	its derivatives	181:195	its derivatives	181:195	Alginate and its derivatives are widely used as food additives and dietary fibers.					
34048831	1	37	used	used	208:211	arg2	fibers					243:248	dietary fibers	235:248	dietary fibers	235:248	Alginate and its derivatives are widely used as food additives and dietary fibers.					
34048831	3	38	theme	polysaccharides	487:501	arg1	outcomes					469:476	the fermentation outcomes	452:476	the fermentation outcomes of these polysaccharides	452:501	However, how different compositions of the microbiota may affect the fermentation outcomes of these polysaccharides remains unknown.					
34048831	6	39	theme	Bacteroides-dominated	945:965	arg1	microbiota					967:976	Bacteroides-dominated microbiota	945:976	Bacteroides-dominated microbiota	945:976	Fermentation of alginate and PM by Bacteroides-dominated microbiota produced the highest amount of total SCFAs and butyrate.					
34048831	7	40	theme	enterotype-specific	1061:1079	arg1	effect					1081:1086	an enterotype-specific effect	1058:1086	an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives	1058:1152	Our study demonstrates an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives and highlights that personalized nutrition using dietary fibers should be tailored according to individual's composition of the gut microbiome.					
34048831	1	41	theme	dietary	235:241	arg1	fibers					243:248	dietary fibers	235:248	dietary fibers	235:248	Alginate and its derivatives are widely used as food additives and dietary fibers.					
34048831	1	41	theme	dietary	235:241	arg1	Alginate					168:175	Alginate	168:175	Alginate	168:175	Alginate and its derivatives are widely used as food additives and dietary fibers.					
34048831	1	41	theme	dietary	235:241	arg1	derivatives					185:195	its derivatives	181:195	its derivatives	181:195	Alginate and its derivatives are widely used as food additives and dietary fibers.					
34048831	7	42	theme	gut	1282:1284	arg1	microbiome					1286:1295	the gut microbiome	1278:1295	the gut microbiome	1278:1295	Our study demonstrates an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives and highlights that personalized nutrition using dietary fibers should be tailored according to individual's composition of the gut microbiome.					
34048831	7	43	theme	microbiome	1286:1295	arg1	composition					1263:1273	individual's composition	1250:1273	individual's composition of the gut microbiome	1250:1295	Our study demonstrates an enterotype-specific effect of microbiota on the fermentation of alginate and its derivatives and highlights that personalized nutrition using dietary fibers should be tailored according to individual's composition of the gut microbiome.					
34048831	2	44	theme	Previous	251:258	arg1	studies					260:266	Previous studies	251:266	Previous studies	251:266	Previous studies indicated that alginate, polyguluronate (PG) and polymannuronate acid (PM) could be fermented by human gut microbiota.					
34048831	4	45	theme	Prevotella	689:698	arg1	Prevotella-dominated					667:686	Prevotella-dominated	667:686	Prevotella-dominated (Prevotella enterotype)	667:710	Here we show that Bacteroides-dominated microbiota (Bacteroides enterotype) is more proficient at degrading and utilizing PG and PM as compared to Prevotella-dominated (Prevotella enterotype) and Escherichia-dominated microbiota (Escherichia enterotype).					
34048831	4	45	theme	Prevotella	689:698	arg1	enterotype					700:709	Prevotella enterotype	689:709	Prevotella enterotype	689:709	Here we show that Bacteroides-dominated microbiota (Bacteroides enterotype) is more proficient at degrading and utilizing PG and PM as compared to Prevotella-dominated (Prevotella enterotype) and Escherichia-dominated microbiota (Escherichia enterotype).					
34048831	4	46	theme	Escherichia-dominated	716:736	arg1	microbiota					738:747	Escherichia-dominated microbiota	716:747	Escherichia-dominated microbiota (Escherichia enterotype)	716:772	Here we show that Bacteroides-dominated microbiota (Bacteroides enterotype) is more proficient at degrading and utilizing PG and PM as compared to Prevotella-dominated (Prevotella enterotype) and Escherichia-dominated microbiota (Escherichia enterotype).					
32507173	7	0	theme	crosslinking	1098:1109	arg1	properties					1111:1120	crosslinking properties	1098:1120	crosslinking properties	1098:1120	Finally, the optimal condition (160 °C, pH 7 for 10 min) was used in a repeated SWE procedure to maximize the yield and crosslinking properties.					
32507173	1	1	theme	great	177:181	arg1	potential					183:191	great potential	177:191	great potential	177:191	Feruloylated arabinoxylans demonstrate great potential in developing natural materials through oxidative crosslinking.					
32507173	7	2	theme	optimal	991:997	arg1	condition					999:1007	the optimal condition	987:1007	the optimal condition (160 °C, pH 7 for 10 min)	987:1033	Finally, the optimal condition (160 °C, pH 7 for 10 min) was used in a repeated SWE procedure to maximize the yield and crosslinking properties.					
32507173	8	3	theme	water	1154:1158	arg1	combination					1127:1137	The combination	1123:1137	The combination of subcritical water and enzymatic crosslinking	1123:1185	The combination of subcritical water and enzymatic crosslinking presents as a promising green approach for preparing hydrogels from herbaceous hemicelluloses.					
32507173	8	4	theme	subcritical	1142:1152	arg1	water					1154:1158	subcritical water	1142:1158	subcritical water	1142:1158	The combination of subcritical water and enzymatic crosslinking presents as a promising green approach for preparing hydrogels from herbaceous hemicelluloses.					
32507173	7	5	dep	condition	999:1007	arg1	pH					1018:1019	pH 7	1018:1021	pH 7	1018:1021	Finally, the optimal condition (160 °C, pH 7 for 10 min) was used in a repeated SWE procedure to maximize the yield and crosslinking properties.					
32507173	7	5	dep	condition	999:1007	arg1	160 °C					1010:1015	160 °C	1010:1015	160 °C	1010:1015	Finally, the optimal condition (160 °C, pH 7 for 10 min) was used in a repeated SWE procedure to maximize the yield and crosslinking properties.					
32507173	4	6	theme	molecular	640:648	arg1	yields					605:610	Various yields	597:610	Various yields	597:610	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	6	theme	molecular	640:648	arg1	compositions					622:633	chemical compositions	613:633	chemical compositions	613:633	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	6	theme	molecular	640:648	arg1	weights					650:656	molecular weights	640:656	molecular weights	640:656	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	6	theme	molecular	640:648	arg1	conditions					683:692	the SWE conditions	675:692	the SWE conditions changed	675:700	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	0	7	theme	subcritical	92:102	arg1	water					104:108	subcritical water	92:108	subcritical water	92:108	Facile and green preparation of diverse arabinoxylan hydrogels from wheat bran by combining subcritical water and enzymatic crosslinking.					
32507173	6	8	theme	broad	917:921	arg1	spectrum					923:930	a broad spectrum	915:930	a broad spectrum of gelling ability	915:949	Laccase-induced oxidative crosslinking was investigated through dynamic rheology, finding a broad spectrum of gelling ability for the SWE arabinoxylans.					
32507173	7	9	theme	repeated	1049:1056	arg1	procedure					1062:1070	a repeated SWE procedure	1047:1070	a repeated SWE procedure	1047:1070	Finally, the optimal condition (160 °C, pH 7 for 10 min) was used in a repeated SWE procedure to maximize the yield and crosslinking properties.					
32507173	7	10	theme	SWE	1058:1060	arg1	procedure					1062:1070	a repeated SWE procedure	1047:1070	a repeated SWE procedure	1047:1070	Finally, the optimal condition (160 °C, pH 7 for 10 min) was used in a repeated SWE procedure to maximize the yield and crosslinking properties.					
32507173	2	11	theme	heavy	325:329	arg1	losses					331:336	heavy losses	325:336	heavy losses of feruloyl esters	325:355	However, the common alkaline method to extract arabinoxylans causes heavy losses of feruloyl esters thus hinders the crosslinking activity.					
32507173	3	12	theme	subcritical	496:506	arg1	SWE					526:528	SWE	526:528	SWE	526:528	Highly feruloylated arabinoxylans were fractionated from wheat bran by an alternative method using subcritical water extraction (SWE) and were compared with water- and alkali-extracted arabinoxylans.					
32507173	3	12	theme	subcritical	496:506	arg1	extraction					514:523	subcritical water extraction	496:523	subcritical water extraction (SWE)	496:529	Highly feruloylated arabinoxylans were fractionated from wheat bran by an alternative method using subcritical water extraction (SWE) and were compared with water- and alkali-extracted arabinoxylans.					
32507173	8	13	theme	herbaceous	1255:1264	arg1	hemicelluloses					1266:1279	herbaceous hemicelluloses	1255:1279	herbaceous hemicelluloses	1255:1279	The combination of subcritical water and enzymatic crosslinking presents as a promising green approach for preparing hydrogels from herbaceous hemicelluloses.					
32507173	3	14	theme	wheat	454:458	arg1	bran					460:463	wheat bran	454:463	wheat bran	454:463	Highly feruloylated arabinoxylans were fractionated from wheat bran by an alternative method using subcritical water extraction (SWE) and were compared with water- and alkali-extracted arabinoxylans.					
32507173	3	15	theme	water	508:512	arg1	SWE					526:528	SWE	526:528	SWE	526:528	Highly feruloylated arabinoxylans were fractionated from wheat bran by an alternative method using subcritical water extraction (SWE) and were compared with water- and alkali-extracted arabinoxylans.					
32507173	3	15	theme	water	508:512	arg1	extraction					514:523	subcritical water extraction	496:523	subcritical water extraction (SWE)	496:529	Highly feruloylated arabinoxylans were fractionated from wheat bran by an alternative method using subcritical water extraction (SWE) and were compared with water- and alkali-extracted arabinoxylans.					
32507173	5	16	theme	Methylation	703:713	arg1	analysis					715:722	Methylation analysis	703:722	Methylation analysis	703:722	Methylation analysis showed that the SWE arabinoxylans had a similar linkage pattern as the water-extracted arabinoxylan.					
32507173	7	17	used	used	1039:1042	arg2	condition					999:1007	the optimal condition	987:1007	the optimal condition (160 °C, pH 7 for 10 min)	987:1033	Finally, the optimal condition (160 °C, pH 7 for 10 min) was used in a repeated SWE procedure to maximize the yield and crosslinking properties.					
32507173	1	18	theme	natural	207:213	arg1	materials					215:223	natural materials	207:223	natural materials	207:223	Feruloylated arabinoxylans demonstrate great potential in developing natural materials through oxidative crosslinking.					
32507173	0	19	theme	Facile	0:5	arg1	preparation					17:27	Facile and green preparation	0:27	Facile and green preparation of diverse arabinoxylan	0:51	Facile and green preparation of diverse arabinoxylan hydrogels from wheat bran by combining subcritical water and enzymatic crosslinking.					
32507173	6	20	theme	dynamic	889:895	arg1	rheology					897:904	dynamic rheology	889:904	dynamic rheology	889:904	Laccase-induced oxidative crosslinking was investigated through dynamic rheology, finding a broad spectrum of gelling ability for the SWE arabinoxylans.					
32507173	2	21	theme	esters	350:355	arg1	losses					331:336	heavy losses	325:336	heavy losses of feruloyl esters	325:355	However, the common alkaline method to extract arabinoxylans causes heavy losses of feruloyl esters thus hinders the crosslinking activity.					
32507173	5	22	theme	water-extracted	795:809	arg1	pattern					780:786	a similar linkage pattern	762:786	a similar linkage pattern	762:786	Methylation analysis showed that the SWE arabinoxylans had a similar linkage pattern as the water-extracted arabinoxylan.					
32507173	5	22	theme	water-extracted	795:809	arg1	arabinoxylan					811:822	the water-extracted arabinoxylan	791:822	the water-extracted arabinoxylan	791:822	Methylation analysis showed that the SWE arabinoxylans had a similar linkage pattern as the water-extracted arabinoxylan.					
32507173	4	23	theme	chemical	613:620	arg1	yields					605:610	Various yields	597:610	Various yields	597:610	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	23	theme	chemical	613:620	arg1	compositions					622:633	chemical compositions	613:633	chemical compositions	613:633	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	23	theme	chemical	613:620	arg1	weights					650:656	molecular weights	640:656	molecular weights	640:656	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	23	theme	chemical	613:620	arg1	conditions					683:692	the SWE conditions	675:692	the SWE conditions changed	675:700	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	3	24	theme	feruloylated	404:415	arg1	arabinoxylans					417:429	Highly feruloylated arabinoxylans	397:429	Highly feruloylated arabinoxylans	397:429	Highly feruloylated arabinoxylans were fractionated from wheat bran by an alternative method using subcritical water extraction (SWE) and were compared with water- and alkali-extracted arabinoxylans.					
32507173	2	25	theme	feruloyl	341:348	arg1	esters					350:355	feruloyl esters	341:355	feruloyl esters	341:355	However, the common alkaline method to extract arabinoxylans causes heavy losses of feruloyl esters thus hinders the crosslinking activity.					
32507173	0	26	theme	green	11:15	arg1	preparation					17:27	Facile and green preparation	0:27	Facile and green preparation of diverse arabinoxylan	0:51	Facile and green preparation of diverse arabinoxylan hydrogels from wheat bran by combining subcritical water and enzymatic crosslinking.					
32507173	0	27	theme	enzymatic	114:122	arg1	crosslinking					124:135	enzymatic crosslinking	114:135	enzymatic crosslinking	114:135	Facile and green preparation of diverse arabinoxylan hydrogels from wheat bran by combining subcritical water and enzymatic crosslinking.					
32507173	1	28	theme	oxidative	233:241	arg1	crosslinking					243:254	oxidative crosslinking	233:254	oxidative crosslinking	233:254	Feruloylated arabinoxylans demonstrate great potential in developing natural materials through oxidative crosslinking.					
32507173	0	29	theme	diverse	32:38	arg1	arabinoxylan					40:51	diverse arabinoxylan	32:51	diverse arabinoxylan	32:51	Facile and green preparation of diverse arabinoxylan hydrogels from wheat bran by combining subcritical water and enzymatic crosslinking.					
32507173	8	30	theme	crosslinking	1174:1185	arg1	combination					1127:1137	The combination	1123:1137	The combination of subcritical water and enzymatic crosslinking	1123:1185	The combination of subcritical water and enzymatic crosslinking presents as a promising green approach for preparing hydrogels from herbaceous hemicelluloses.					
32507173	2	31	theme	common	270:275	arg1	method					286:291	the common alkaline method	266:291	the common alkaline method to extract arabinoxylans	266:316	However, the common alkaline method to extract arabinoxylans causes heavy losses of feruloyl esters thus hinders the crosslinking activity.					
32507173	3	32	theme	alternative	471:481	arg1	method					483:488	an alternative method	468:488	an alternative method using subcritical water extraction (SWE)	468:529	Highly feruloylated arabinoxylans were fractionated from wheat bran by an alternative method using subcritical water extraction (SWE) and were compared with water- and alkali-extracted arabinoxylans.					
32507173	4	33	theme	Various	597:603	arg1	yields					605:610	Various yields	597:610	Various yields	597:610	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	33	theme	Various	597:603	arg1	compositions					622:633	chemical compositions	613:633	chemical compositions	613:633	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	33	theme	Various	597:603	arg1	weights					650:656	molecular weights	640:656	molecular weights	640:656	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	33	theme	Various	597:603	arg1	conditions					683:692	the SWE conditions	675:692	the SWE conditions changed	675:700	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	8	34	theme	enzymatic	1164:1172	arg1	crosslinking					1174:1185	enzymatic crosslinking	1164:1185	enzymatic crosslinking	1164:1185	The combination of subcritical water and enzymatic crosslinking presents as a promising green approach for preparing hydrogels from herbaceous hemicelluloses.					
32507173	6	35	theme	SWE	959:961	arg1	arabinoxylans					963:975	the SWE arabinoxylans	955:975	the SWE arabinoxylans	955:975	Laccase-induced oxidative crosslinking was investigated through dynamic rheology, finding a broad spectrum of gelling ability for the SWE arabinoxylans.					
32507173	3	36	theme	water-	554:559	arg1	arabinoxylans					582:594	water- and alkali-extracted arabinoxylans	554:594	water- and alkali-extracted arabinoxylans	554:594	Highly feruloylated arabinoxylans were fractionated from wheat bran by an alternative method using subcritical water extraction (SWE) and were compared with water- and alkali-extracted arabinoxylans.					
32507173	0	37	theme	arabinoxylan	40:51	arg1	preparation					17:27	Facile and green preparation	0:27	Facile and green preparation of diverse arabinoxylan	0:51	Facile and green preparation of diverse arabinoxylan hydrogels from wheat bran by combining subcritical water and enzymatic crosslinking.					
32507173	5	38	contain	had	758:760	arg1	arabinoxylans					744:756	the SWE arabinoxylans	736:756	the SWE arabinoxylans	736:756	Methylation analysis showed that the SWE arabinoxylans had a similar linkage pattern as the water-extracted arabinoxylan.					
32507173	5	38	contain	had	758:760	arg2	pattern					780:786	a similar linkage pattern	762:786	a similar linkage pattern	762:786	Methylation analysis showed that the SWE arabinoxylans had a similar linkage pattern as the water-extracted arabinoxylan.					
32507173	5	38	contain	had	758:760	arg2	arabinoxylan					811:822	the water-extracted arabinoxylan	791:822	the water-extracted arabinoxylan	791:822	Methylation analysis showed that the SWE arabinoxylans had a similar linkage pattern as the water-extracted arabinoxylan.					
32507173	5	39	theme	linkage	772:778	arg1	pattern					780:786	a similar linkage pattern	762:786	a similar linkage pattern	762:786	Methylation analysis showed that the SWE arabinoxylans had a similar linkage pattern as the water-extracted arabinoxylan.					
32507173	5	39	theme	linkage	772:778	arg1	arabinoxylan					811:822	the water-extracted arabinoxylan	791:822	the water-extracted arabinoxylan	791:822	Methylation analysis showed that the SWE arabinoxylans had a similar linkage pattern as the water-extracted arabinoxylan.					
32507173	8	40	from	hemicelluloses	1266:1279	arg1	hydrogels					1240:1248	hydrogels	1240:1248	hydrogels from herbaceous hemicelluloses	1240:1279	The combination of subcritical water and enzymatic crosslinking presents as a promising green approach for preparing hydrogels from herbaceous hemicelluloses.					
32507173	2	41	theme	crosslinking	374:385	arg1	activity					387:394	the crosslinking activity	370:394	the crosslinking activity	370:394	However, the common alkaline method to extract arabinoxylans causes heavy losses of feruloyl esters thus hinders the crosslinking activity.					
32507173	8	42	theme	promising	1201:1209	arg1	approach					1217:1224	a promising green approach	1199:1224	a promising green approach for preparing hydrogels from herbaceous hemicelluloses	1199:1279	The combination of subcritical water and enzymatic crosslinking presents as a promising green approach for preparing hydrogels from herbaceous hemicelluloses.					
32507173	6	43	theme	ability	943:949	arg1	spectrum					923:930	a broad spectrum	915:930	a broad spectrum of gelling ability	915:949	Laccase-induced oxidative crosslinking was investigated through dynamic rheology, finding a broad spectrum of gelling ability for the SWE arabinoxylans.					
32507173	2	44	theme	extract	296:302	arg1	arabinoxylans					304:316	extract arabinoxylans	296:316	extract arabinoxylans	296:316	However, the common alkaline method to extract arabinoxylans causes heavy losses of feruloyl esters thus hinders the crosslinking activity.					
32507173	6	45	theme	oxidative	841:849	arg1	crosslinking					851:862	Laccase-induced oxidative crosslinking	825:862	Laccase-induced oxidative crosslinking	825:862	Laccase-induced oxidative crosslinking was investigated through dynamic rheology, finding a broad spectrum of gelling ability for the SWE arabinoxylans.					
32507173	5	46	theme	similar	764:770	arg1	pattern					780:786	a similar linkage pattern	762:786	a similar linkage pattern	762:786	Methylation analysis showed that the SWE arabinoxylans had a similar linkage pattern as the water-extracted arabinoxylan.					
32507173	5	46	theme	similar	764:770	arg1	arabinoxylan					811:822	the water-extracted arabinoxylan	791:822	the water-extracted arabinoxylan	791:822	Methylation analysis showed that the SWE arabinoxylans had a similar linkage pattern as the water-extracted arabinoxylan.					
32507173	6	47	theme	gelling	935:941	arg1	ability					943:949	gelling ability	935:949	gelling ability	935:949	Laccase-induced oxidative crosslinking was investigated through dynamic rheology, finding a broad spectrum of gelling ability for the SWE arabinoxylans.					
32507173	6	48	theme	Laccase-induced	825:839	arg1	crosslinking					851:862	Laccase-induced oxidative crosslinking	825:862	Laccase-induced oxidative crosslinking	825:862	Laccase-induced oxidative crosslinking was investigated through dynamic rheology, finding a broad spectrum of gelling ability for the SWE arabinoxylans.					
32507173	1	49	theme	Feruloylated	138:149	arg1	arabinoxylans					151:163	Feruloylated arabinoxylans	138:163	Feruloylated arabinoxylans	138:163	Feruloylated arabinoxylans demonstrate great potential in developing natural materials through oxidative crosslinking.					
32507173	0	50	theme	wheat	68:72	arg1	bran					74:77	wheat bran	68:77	wheat bran	68:77	Facile and green preparation of diverse arabinoxylan hydrogels from wheat bran by combining subcritical water and enzymatic crosslinking.					
32507173	3	51	theme	alkali-extracted	565:580	arg1	arabinoxylans					582:594	water- and alkali-extracted arabinoxylans	554:594	water- and alkali-extracted arabinoxylans	554:594	Highly feruloylated arabinoxylans were fractionated from wheat bran by an alternative method using subcritical water extraction (SWE) and were compared with water- and alkali-extracted arabinoxylans.					
32507173	8	52	theme	green	1211:1215	arg1	approach					1217:1224	a promising green approach	1199:1224	a promising green approach for preparing hydrogels from herbaceous hemicelluloses	1199:1279	The combination of subcritical water and enzymatic crosslinking presents as a promising green approach for preparing hydrogels from herbaceous hemicelluloses.					
32507173	2	53	theme	alkaline	277:284	arg1	method					286:291	the common alkaline method	266:291	the common alkaline method to extract arabinoxylans	266:316	However, the common alkaline method to extract arabinoxylans causes heavy losses of feruloyl esters thus hinders the crosslinking activity.					
32507173	4	54	theme	SWE	679:681	arg1	yields					605:610	Various yields	597:610	Various yields	597:610	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	54	theme	SWE	679:681	arg1	compositions					622:633	chemical compositions	613:633	chemical compositions	613:633	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	54	theme	SWE	679:681	arg1	weights					650:656	molecular weights	640:656	molecular weights	640:656	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
32507173	4	54	theme	SWE	679:681	arg1	conditions					683:692	the SWE conditions	675:692	the SWE conditions changed	675:700	Various yields, chemical compositions, and molecular weights were obtained as the SWE conditions changed.					
34339343	0	0	theme	monosaccharide	80:93	arg1	compositions					95:106	monosaccharide compositions	80:106	monosaccharide compositions	80:106	Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity.					
34339343	0	1	from	Tetrastigma	21:31	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg	0:57	Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity.					
34339343	4	2	dep	dominant	916:923	arg1	%					940:940	26.749 ± 0.634%	926:940	26.749 ± 0.634%	926:940	THP was consisted of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose, and arabinose, while glucose was the dominant (26.749 ± 0.634%).					
34339343	2	3	theme	response	388:395	arg1	RSM					418:420	RSM	418:420	RSM	418:420	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	3	theme	response	388:395	arg1	methodology					405:415	response surface methodology	388:415	response surface methodology (RSM)	388:421	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	5	4	theme	antioxidant	1079:1089	arg1	activity					1091:1098	strong antioxidant activity	1072:1098	strong antioxidant activity	1072:1098	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	6	5	theme	novel	1189:1193	arg1	antioxidant					1195:1205	a novel antioxidant	1187:1205	a novel antioxidant	1187:1205	The results indicated that THP has the potential to be a novel antioxidant and could expand its application in food and medicine.					
34339343	2	6	theme	X	513:513	arg1	dosage					516:521	(X) dosage	512:521	(X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material	512:617	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	6	theme	X	513:513	arg1	variables					497:505	the independent variables	481:505	the independent variables	481:505	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	7	theme	power	573:577	arg1	dosage					516:521	(X) dosage	512:521	(X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material	512:617	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	7	theme	power	573:577	arg1	variables					497:505	the independent variables	481:505	the independent variables	481:505	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	5	8	with	assay	1054:1058	arg1	method					1008:1013	the FRAP method	999:1013	the FRAP method	999:1013	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	5	9	with	DPPH	1016:1019	arg1	method					1008:1013	the FRAP method	999:1013	the FRAP method	999:1013	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	2	10	theme	ultrasonic	562:571	arg1	power					573:577	(X) ultrasonic power	558:577	(X) ultrasonic power	558:577	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	11	theme	water	597:601	arg1	cellulase					526:534	cellulase	526:534	cellulase	526:534	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	11	theme	water	597:601	arg1	time					552:555	(X) extraction time	537:555	(X) extraction time	537:555	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	11	theme	water	597:601	arg1	power					573:577	(X) ultrasonic power	558:577	(X) ultrasonic power	558:577	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	11	theme	water	597:601	arg1	ratio					588:592	(X) ratio	584:592	(X) ratio of water to the material	584:617	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	6	12	contain	has	1163:1165	arg2	potential					1171:1179	the potential to be a novel antioxidant	1167:1205	the potential to be a novel antioxidant	1167:1205	The results indicated that THP has the potential to be a novel antioxidant and could expand its application in food and medicine.					
34339343	6	12	contain	has	1163:1165	arg1	THP					1159:1161	THP	1159:1161	THP	1159:1161	The results indicated that THP has the potential to be a novel antioxidant and could expand its application in food and medicine.					
34339343	1	13	theme	monosaccharide	273:286	arg1	compositions					288:299	its monosaccharide compositions	269:299	its monosaccharide compositions	269:299	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	0	14	theme	antioxidant	113:123	arg1	activity					125:132	antioxidant activity	113:132	antioxidant activity	113:132	Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity.					
34339343	6	15	from	application	1228:1238	arg1	food					1243:1246	food	1243:1246	food	1243:1246	The results indicated that THP has the potential to be a novel antioxidant and could expand its application in food and medicine.					
34339343	6	15	from	application	1228:1238	arg1	medicine					1252:1259	medicine	1252:1259	medicine	1252:1259	The results indicated that THP has the potential to be a novel antioxidant and could expand its application in food and medicine.					
34339343	2	16	theme	ratio	588:592	arg1	dosage					516:521	(X) dosage	512:521	(X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material	512:617	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	16	theme	ratio	588:592	arg1	variables					497:505	the independent variables	481:505	the independent variables	481:505	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	3	17	theme	predicted	757:765	arg1	value					767:771	the predicted value	753:771	the predicted value	753:771	The extraction rate of THP was increased effectively under the optimum conditions, and the maximum (4.692 ± 0.059%) was well-matched the predicted value from RSM.					
34339343	5	18	theme	FRAP	1003:1006	arg1	method					1008:1013	the FRAP method	999:1013	the FRAP method	999:1013	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	1	19	theme	Diels	193:197	arg1	THP					224:226	THP	224:226	THP	224:226	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	1	19	theme	Diels	193:197	arg1	polysaccharides					207:221	Tetrastigma hemsleyanum Diels et Gilg polysaccharides	169:221	Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP)	169:227	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	2	20	theme	X	585:585	arg1	ratio					588:592	(X) ratio	584:592	(X) ratio of water to the material	584:617	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	4	21	theme	glucuronic	813:822	arg1	acid					824:827	glucuronic acid	813:827	glucuronic acid	813:827	THP was consisted of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose, and arabinose, while glucose was the dominant (26.749 ± 0.634%).					
34339343	1	22	theme	Gilg	202:205	arg1	THP					224:226	THP	224:226	THP	224:226	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	1	22	theme	Gilg	202:205	arg1	polysaccharides					207:221	Tetrastigma hemsleyanum Diels et Gilg polysaccharides	169:221	Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP)	169:227	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	2	23	theme	time	552:555	arg1	dosage					516:521	(X) dosage	512:521	(X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material	512:617	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	23	theme	time	552:555	arg1	variables					497:505	the independent variables	481:505	the independent variables	481:505	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	0	24	dep	Diels	45:49	arg1	Gilg					54:57	Diels et Gilg	45:57	Tetrastigma hemsleyanum Diels et Gilg	21:57	Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity.					
34339343	0	25	dep	extraction	68:77	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg	0:57	Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity.					
34339343	5	26	with	assay	988:992	arg1	method					1008:1013	the FRAP method	999:1013	the FRAP method	999:1013	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	1	27	theme	polysaccharides	207:221	arg1	extraction					155:164	extraction	155:164	extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP)	155:227	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	1	28	theme	antioxidant	305:315	arg1	activity					317:324	antioxidant activity	305:324	antioxidant activity	305:324	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	2	29	theme	extraction	541:550	arg1	time					552:555	(X) extraction time	537:555	(X) extraction time	537:555	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	5	30	theme	total	961:965	arg1	capacity					979:986	the total antioxidant capacity	957:986	the total antioxidant capacity assay with the FRAP method	957:1013	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	5	31	theme	strong	1072:1077	arg1	activity					1091:1098	strong antioxidant activity	1072:1098	strong antioxidant activity	1072:1098	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	3	32	from	value	767:771	arg1	maximum					711:717	the maximum	707:717	the maximum (4.692 ± 0.059%)	707:734	The extraction rate of THP was increased effectively under the optimum conditions, and the maximum (4.692 ± 0.059%) was well-matched the predicted value from RSM.					
34339343	3	32	from	value	767:771	arg1	well-matched					740:751	well-matched	740:751	well-matched	740:751	The extraction rate of THP was increased effectively under the optimum conditions, and the maximum (4.692 ± 0.059%) was well-matched the predicted value from RSM.					
34339343	5	33	theme	antioxidant	967:977	arg1	capacity					979:986	the total antioxidant capacity	957:986	the total antioxidant capacity assay with the FRAP method	957:1013	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	5	34	theme	hydroxyl	1026:1033	arg1	radical					1035:1041	hydroxyl radical	1026:1041	hydroxyl radical scavenging assay	1026:1058	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	2	35	theme	X	538:538	arg1	time					552:555	(X) extraction time	537:555	(X) extraction time	537:555	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	5	36	theme	capacity	979:986	arg1	assay					988:992	the total antioxidant capacity assay	957:992	the total antioxidant capacity assay with the FRAP method	957:1013	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	2	37	theme	independent	485:495	arg1	dosage					516:521	(X) dosage	512:521	(X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material	512:617	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	37	theme	independent	485:495	arg1	variables					497:505	the independent variables	481:505	the independent variables	481:505	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	5	38	theme	radical	1035:1041	arg1	assay					1054:1058	hydroxyl radical scavenging assay	1026:1058	hydroxyl radical scavenging assay	1026:1058	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	5	39	theme	scavenging	1043:1052	arg1	assay					1054:1058	hydroxyl radical scavenging assay	1026:1058	hydroxyl radical scavenging assay	1026:1058	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	2	40	theme	X	559:559	arg1	power					573:577	(X) ultrasonic power	558:577	(X) ultrasonic power	558:577	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	1	41	dep	Diels	193:197	arg1	et					199:200	Tetrastigma hemsleyanum Diels et	169:200	Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP)	169:227	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	2	42	theme	Single-factor	358:370	arg1	experiments					372:382	Single-factor experiments	358:382	Single-factor experiments	358:382	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	3	43	theme	extraction	624:633	arg1	rate					635:638	The extraction rate	620:638	The extraction rate of THP	620:645	The extraction rate of THP was increased effectively under the optimum conditions, and the maximum (4.692 ± 0.059%) was well-matched the predicted value from RSM.					
34339343	3	44	theme	optimum	683:689	arg1	conditions					691:700	the optimum conditions	679:700	the optimum conditions	679:700	The extraction rate of THP was increased effectively under the optimum conditions, and the maximum (4.692 ± 0.059%) was well-matched the predicted value from RSM.					
34339343	5	45	theme	dose-dependent	1107:1120	arg1	behavior					1122:1129	a dose-dependent behavior	1105:1129	a dose-dependent behavior	1105:1129	According to the total antioxidant capacity assay with the FRAP method, DPPH, and hydroxyl radical scavenging assay, THP showed strong antioxidant activity with a dose-dependent behavior.					
34339343	3	46	dep	value	767:771	arg1	RSM					778:780	RSM	778:780	RSM	778:780	The extraction rate of THP was increased effectively under the optimum conditions, and the maximum (4.692 ± 0.059%) was well-matched the predicted value from RSM.					
34339343	0	47	dep	Tetrastigma	21:31	arg1	hemsleyanum					33:43	Tetrastigma hemsleyanum Diels et Gilg	21:57	Tetrastigma hemsleyanum Diels et Gilg	21:57	Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity.					
34339343	0	47	dep	Tetrastigma	21:31	arg1	Diels					45:49	Diels	45:49	Diels	45:49	Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity.					
34339343	2	48	theme	surface	397:403	arg1	RSM					418:420	RSM	418:420	RSM	418:420	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	48	theme	surface	397:403	arg1	methodology					405:415	response surface methodology	388:415	response surface methodology (RSM)	388:421	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	1	49	theme	ultrasonic	235:244	arg1	method					258:263	ultrasonic with enzyme method	235:263	ultrasonic with enzyme method	235:263	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	2	50	theme	cellulase	526:534	arg1	dosage					516:521	(X) dosage	512:521	(X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material	512:617	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	2	50	theme	cellulase	526:534	arg1	variables					497:505	the independent variables	481:505	the independent variables	481:505	Single-factor experiments and response surface methodology (RSM) were performed to optimize conditions for extraction, and the independent variables were (X) dosage of cellulase, (X) extraction time, (X) ultrasonic power, and (X) ratio of water to the material.					
34339343	4	51	theme	galacturonic	840:851	arg1	acid					853:856	galacturonic acid	840:856	galacturonic acid	840:856	THP was consisted of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose, and arabinose, while glucose was the dominant (26.749 ± 0.634%).					
34339343	1	52	theme	with	246:249	arg1	method					258:263	ultrasonic with enzyme method	235:263	ultrasonic with enzyme method	235:263	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	0	53	theme	optimum	60:66	arg1	extraction					68:77	optimum extraction	60:77	optimum extraction	60:77	Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: optimum extraction, monosaccharide compositions, and antioxidant activity.					
34339343	3	54	theme	THP	643:645	arg1	rate					635:638	The extraction rate	620:638	The extraction rate of THP	620:645	The extraction rate of THP was increased effectively under the optimum conditions, and the maximum (4.692 ± 0.059%) was well-matched the predicted value from RSM.					
34339343	1	55	theme	extraction	155:164	arg1	optimization					139:150	The optimization	135:150	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity	135:324	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
34339343	1	56	theme	enzyme	251:256	arg1	method					258:263	ultrasonic with enzyme method	235:263	ultrasonic with enzyme method	235:263	The optimization of extraction of Tetrastigma hemsleyanum Diels et Gilg polysaccharides (THP) using ultrasonic with enzyme method and its monosaccharide compositions and antioxidant activity were investigated in this work.					
32781117	4	0	theme	NSK	427:429	arg1	polysaccharide					431:444	NSK polysaccharide	427:444	NSK polysaccharide	427:444	Here, we used NSK polysaccharide and NSK powder to investigate the bioactivity on the gut microbiota of C57BL/6j mice, respectively.					
32781117	3	1	used	used	284:287	arg2	food					292:295	food	292:295	food	292:295	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	1	used	used	284:287	arg2	NSK					270:272	NSK	270:272	NSK	270:272	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	1	used	used	284:287	arg2	Kütz					264:267	Nostoc sphaeroids Kütz	246:267	Nostoc sphaeroids Kütz (NSK)	246:273	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	1	used	used	284:287	arg2	medicine					301:308	medicine	301:308	medicine	301:308	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	8	2	theme	powder	1050:1055	arg1	group					1057:1061	NSK powder group	1046:1061	NSK powder group	1046:1061	Moreover, we found the IL-1β and IL-6 decreased significantly while TNF-α and IL-10 increased significantly especially in NSK powder group.					
32781117	4	3	theme	NSK	450:452	arg1	powder					454:459	NSK powder	450:459	NSK powder	450:459	Here, we used NSK polysaccharide and NSK powder to investigate the bioactivity on the gut microbiota of C57BL/6j mice, respectively.					
32781117	6	4	theme	Escherichia-Shigella	730:749	arg1	abundance					687:695	the abundance	683:695	the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level	683:783	However, the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level were significantly increased by NSK polysaccharide and NSK powder.					
32781117	10	5	theme	Nostoc	1393:1398	arg1	Kütz					1411:1414	Nostoc sphaeroids Kütz	1393:1414	Nostoc sphaeroids Kütz	1393:1414	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	4	6	from	bioactivity	480:490	arg1	microbiota					503:512	the gut microbiota	495:512	the gut microbiota	495:512	Here, we used NSK polysaccharide and NSK powder to investigate the bioactivity on the gut microbiota of C57BL/6j mice, respectively.					
32781117	3	7	from	bioactivity	362:372	arg1	microbiota					381:390	gut microbiota	377:390	gut microbiota	377:390	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	10	8	theme	community	1345:1353	arg1	"					1354:1354	core bacterial community"	1330:1354	a "core bacterial community"	1327:1354	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	4	9	theme	mice	526:529	arg1	microbiota					503:512	the gut microbiota	495:512	the gut microbiota	495:512	Here, we used NSK polysaccharide and NSK powder to investigate the bioactivity on the gut microbiota of C57BL/6j mice, respectively.					
32781117	0	10	theme	C57BL/6j	86:93	arg1	mice					95:98	C57BL/6j mice	86:98	C57BL/6j mice	86:98	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	6	11	theme	Parabacteroides	713:727	arg1	abundance					687:695	the abundance	683:695	the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level	683:783	However, the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level were significantly increased by NSK polysaccharide and NSK powder.					
32781117	4	12	theme	C57BL/6j	517:524	arg1	mice					526:529	C57BL/6j mice	517:529	C57BL/6j mice	517:529	Here, we used NSK polysaccharide and NSK powder to investigate the bioactivity on the gut microbiota of C57BL/6j mice, respectively.					
32781117	10	13	theme	biological	1420:1429	arg1	function					1431:1438	biological function	1420:1438	biological function	1420:1438	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	10	14	theme	core	1330:1333	arg1	"					1354:1354	core bacterial community"	1330:1354	a "core bacterial community"	1327:1354	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	6	15	theme	NSK	817:819	arg1	polysaccharide					821:834	NSK polysaccharide	817:834	NSK polysaccharide	817:834	However, the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level were significantly increased by NSK polysaccharide and NSK powder.					
32781117	5	16	theme	ribosomal	553:561	arg1	sequencing					572:581	ribosomal RNA gene sequencing	553:581	ribosomal RNA gene sequencing	553:581	By 16S ribosomal RNA gene sequencing, we found the composition of gut microbiota had been changed and differed from each other.					
32781117	0	17	theme	Nostoc	0:5	arg1	powder					42:47	powder	42:47	powder	42:47	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	0	17	theme	Nostoc	0:5	arg1	polysaccharide					23:36	Kütz polysaccharide	18:36	Kütz polysaccharide	18:36	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	0	17	theme	Nostoc	0:5	arg1	sphaeroids					7:16	Nostoc sphaeroids	0:16	Nostoc sphaeroids Kütz polysaccharide and powder	0:47	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	5	18	theme	RNA	563:565	arg1	sequencing					572:581	ribosomal RNA gene sequencing	553:581	ribosomal RNA gene sequencing	553:581	By 16S ribosomal RNA gene sequencing, we found the composition of gut microbiota had been changed and differed from each other.					
32781117	10	19	theme	new	1364:1366	arg1	evidence					1368:1375	new evidence	1364:1375	new evidence to discover how Nostoc sphaeroids Kütz has biological function	1364:1438	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	3	20	theme	Asian	336:340	arg1	countries					342:350	Asian countries	336:350	Asian countries	336:350	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	5	21	theme	gene	567:570	arg1	sequencing					572:581	ribosomal RNA gene sequencing	553:581	ribosomal RNA gene sequencing	553:581	By 16S ribosomal RNA gene sequencing, we found the composition of gut microbiota had been changed and differed from each other.					
32781117	6	22	theme	genus	773:777	arg1	level					779:783	genus level	773:783	genus level	773:783	However, the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level were significantly increased by NSK polysaccharide and NSK powder.					
32781117	3	23	theme	gut	377:379	arg1	microbiota					381:390	gut microbiota	377:390	gut microbiota	377:390	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	24	theme	sphaeroids	253:262	arg1	NSK					270:272	NSK	270:272	NSK	270:272	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	24	theme	sphaeroids	253:262	arg1	Kütz					264:267	Nostoc sphaeroids Kütz	246:267	Nostoc sphaeroids Kütz (NSK)	246:273	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	24	theme	sphaeroids	253:262	arg1	food					292:295	food	292:295	food	292:295	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	24	theme	sphaeroids	253:262	arg1	medicine					301:308	medicine	301:308	medicine	301:308	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	10	25	theme	sphaeroids	1400:1409	arg1	Kütz					1411:1414	Nostoc sphaeroids Kütz	1393:1414	Nostoc sphaeroids Kütz	1393:1414	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	6	26	from	abundance	687:695	arg1	level					779:783	genus level	773:783	genus level	773:783	However, the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level were significantly increased by NSK polysaccharide and NSK powder.					
32781117	3	27	theme	years	327:331	arg1	thousands					314:322	thousands	314:322	thousands of years	314:331	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	4	28	theme	gut	499:501	arg1	microbiota					503:512	the gut microbiota	495:512	the gut microbiota	495:512	Here, we used NSK polysaccharide and NSK powder to investigate the bioactivity on the gut microbiota of C57BL/6j mice, respectively.					
32781117	1	29	theme	Gut	101:103	arg1	microbiota					105:114	Gut microbiota	101:114	Gut microbiota	101:114	Gut microbiota is the collection of microbes that lives in the host.					
32781117	1	29	theme	Gut	101:103	arg1	collection					123:132	the collection	119:132	the collection of microbes that lives in the host	119:167	Gut microbiota is the collection of microbes that lives in the host.					
32781117	9	30	with	co-related	1180:1189	arg1	IL-1β					1196:1200	IL-1β	1196:1200	IL-1β	1196:1200	Intriguingly, the increased microbes were significantly positively co-related with TNF-α and IL-10 while negatively co-related with IL-1β and IL-6 by co-relation and network analysis.					
32781117	9	30	with	co-related	1180:1189	arg1	IL-6					1206:1209	IL-6	1206:1209	IL-6	1206:1209	Intriguingly, the increased microbes were significantly positively co-related with TNF-α and IL-10 while negatively co-related with IL-1β and IL-6 by co-relation and network analysis.					
32781117	0	31	from	community	73:81	arg1	mice					95:98	C57BL/6j mice	86:98	C57BL/6j mice	86:98	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	10	32	theme	above	1252:1256	arg1	results					1258:1264	The above results	1248:1264	The above results	1248:1264	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	3	33	theme	unclear	395:401	arg1	now					408:410	unclear till now	395:410	unclear till now	395:410	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	33	theme	unclear	395:401	arg1	bioactivity					362:372	the bioactivity	358:372	the bioactivity on gut microbiota	358:390	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	4	34	used	used	422:425	arg2	we					419:420	we	419:420	we	419:420	Here, we used NSK polysaccharide and NSK powder to investigate the bioactivity on the gut microbiota of C57BL/6j mice, respectively.					
32781117	3	35	theme	Nostoc	246:251	arg1	NSK					270:272	NSK	270:272	NSK	270:272	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	35	theme	Nostoc	246:251	arg1	Kütz					264:267	Nostoc sphaeroids Kütz	246:267	Nostoc sphaeroids Kütz (NSK)	246:273	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	35	theme	Nostoc	246:251	arg1	food					292:295	food	292:295	food	292:295	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	35	theme	Nostoc	246:251	arg1	medicine					301:308	medicine	301:308	medicine	301:308	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	5	36	dep	found	587:591	arg1	differed					648:655	differed	648:655	differed from each other	648:671	By 16S ribosomal RNA gene sequencing, we found the composition of gut microbiota had been changed and differed from each other.					
32781117	5	36	dep	found	587:591	arg1	changed					636:642	changed	636:642	had been changed	627:642	By 16S ribosomal RNA gene sequencing, we found the composition of gut microbiota had been changed and differed from each other.					
32781117	3	37	theme	till	403:406	arg1	now					408:410	unclear till now	395:410	unclear till now	395:410	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	3	37	theme	till	403:406	arg1	bioactivity					362:372	the bioactivity	358:372	the bioactivity on gut microbiota	358:390	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	10	38	contain	has	1416:1418	arg1	Kütz					1411:1414	Nostoc sphaeroids Kütz	1393:1414	Nostoc sphaeroids Kütz	1393:1414	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	10	38	contain	has	1416:1418	arg2	function					1431:1438	biological function	1420:1438	biological function	1420:1438	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	8	39	theme	NSK	1046:1048	arg1	group					1057:1061	NSK powder group	1046:1061	NSK powder group	1046:1061	Moreover, we found the IL-1β and IL-6 decreased significantly while TNF-α and IL-10 increased significantly especially in NSK powder group.					
32781117	6	40	theme	NSK	840:842	arg1	powder					844:849	NSK powder	840:849	NSK powder	840:849	However, the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level were significantly increased by NSK polysaccharide and NSK powder.					
32781117	5	41	theme	microbiota	616:625	arg1	composition					597:607	the composition	593:607	the composition of gut microbiota	593:625	By 16S ribosomal RNA gene sequencing, we found the composition of gut microbiota had been changed and differed from each other.					
32781117	2	42	theme	major	184:188	arg1	factor					190:195	the major factor	180:195	the major factor to shape the composition of microbial community	180:243	Glycan is the major factor to shape the composition of microbial community.					
32781117	2	42	theme	major	184:188	arg1	Glycan					170:175	Glycan	170:175	Glycan	170:175	Glycan is the major factor to shape the composition of microbial community.					
32781117	10	43	theme	sphaeroids	1288:1297	arg1	Kütz					1299:1302	Nostoc sphaeroids Kütz	1281:1302	Nostoc sphaeroids Kütz	1281:1302	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	9	44	with	co-related	1131:1140	arg1	IL-10					1157:1161	IL-10	1157:1161	IL-10	1157:1161	Intriguingly, the increased microbes were significantly positively co-related with TNF-α and IL-10 while negatively co-related with IL-1β and IL-6 by co-relation and network analysis.					
32781117	9	44	with	co-related	1131:1140	arg1	TNF-α					1147:1151	TNF-α	1147:1151	TNF-α	1147:1151	Intriguingly, the increased microbes were significantly positively co-related with TNF-α and IL-10 while negatively co-related with IL-1β and IL-6 by co-relation and network analysis.					
32781117	2	45	theme	community	235:243	arg1	composition					210:220	the composition	206:220	the composition of microbial community	206:243	Glycan is the major factor to shape the composition of microbial community.					
32781117	10	46	theme	Nostoc	1281:1286	arg1	Kütz					1299:1302	Nostoc sphaeroids Kütz	1281:1302	Nostoc sphaeroids Kütz	1281:1302	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	5	47	theme	gut	612:614	arg1	microbiota					616:625	gut microbiota	612:625	gut microbiota	612:625	By 16S ribosomal RNA gene sequencing, we found the composition of gut microbiota had been changed and differed from each other.					
32781117	6	48	theme	Parasutterella	755:768	arg1	abundance					687:695	the abundance	683:695	the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level	683:783	However, the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level were significantly increased by NSK polysaccharide and NSK powder.					
32781117	2	49	theme	microbial	225:233	arg1	community					235:243	microbial community	225:243	microbial community	225:243	Glycan is the major factor to shape the composition of microbial community.					
32781117	9	50	theme	increased	1082:1090	arg1	microbes					1092:1099	the increased microbes	1078:1099	the increased microbes	1078:1099	Intriguingly, the increased microbes were significantly positively co-related with TNF-α and IL-10 while negatively co-related with IL-1β and IL-6 by co-relation and network analysis.					
32781117	9	50	theme	increased	1082:1090	arg1	co-related					1131:1140	co-related	1131:1140	co-related	1131:1140	Intriguingly, the increased microbes were significantly positively co-related with TNF-α and IL-10 while negatively co-related with IL-1β and IL-6 by co-relation and network analysis.					
32781117	0	51	theme	bacterial	63:71	arg1	community					73:81	a core bacterial community	56:81	a core bacterial community on C57BL/6j mice	56:98	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	3	52	from	food	292:295	arg1	countries					342:350	Asian countries	336:350	Asian countries	336:350	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
32781117	0	53	dep	sphaeroids	7:16	arg1	powder					42:47	powder	42:47	powder	42:47	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	0	53	dep	sphaeroids	7:16	arg1	polysaccharide					23:36	Kütz polysaccharide	18:36	Kütz polysaccharide	18:36	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	0	53	dep	sphaeroids	7:16	arg1	sphaeroids					7:16	Nostoc sphaeroids	0:16	Nostoc sphaeroids Kütz polysaccharide and powder	0:47	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	10	54	theme	bacterial	1335:1343	arg1	"					1354:1354	core bacterial community"	1330:1354	a "core bacterial community"	1327:1354	The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.					
32781117	0	55	theme	core	58:61	arg1	community					73:81	a core bacterial community	56:81	a core bacterial community on C57BL/6j mice	56:98	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	9	56	theme	network	1230:1236	arg1	analysis					1238:1245	network analysis	1230:1245	network analysis	1230:1245	Intriguingly, the increased microbes were significantly positively co-related with TNF-α and IL-10 while negatively co-related with IL-1β and IL-6 by co-relation and network analysis.					
32781117	0	57	theme	Kütz	18:21	arg1	polysaccharide					23:36	Kütz polysaccharide	18:36	Kütz polysaccharide	18:36	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	0	57	theme	Kütz	18:21	arg1	sphaeroids					7:16	Nostoc sphaeroids	0:16	Nostoc sphaeroids Kütz polysaccharide and powder	0:47	Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.					
32781117	7	58	theme	NSK	912:914	arg1	powder					916:921	NSK powder	912:921	NSK powder	912:921	In addition, Akkermansia and Rikenellaceae were enriched by NSK powder.					
32781117	1	59	theme	microbes	137:144	arg1	microbiota					105:114	Gut microbiota	101:114	Gut microbiota	101:114	Gut microbiota is the collection of microbes that lives in the host.					
32781117	1	59	theme	microbes	137:144	arg1	collection					123:132	the collection	119:132	the collection of microbes that lives in the host	119:167	Gut microbiota is the collection of microbes that lives in the host.					
32781117	3	60	from	medicine	301:308	arg1	countries					342:350	Asian countries	336:350	Asian countries	336:350	Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now.					
33278948	3	0	theme	Nanocomposite	272:284	arg1	polysaccharides					327:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides	272:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides	272:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides can overcome such limitations.					
33278948	4	1	theme	transgenic	480:489	arg1	grain					498:502	transgenic barley grain	480:502	transgenic barley grain	480:502	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	1	2	dep	Thermoplastic	102:114	arg1	polysaccharide-based					117:136	polysaccharide-based	117:136	polysaccharide-based	117:136	Thermoplastic, polysaccharide-based plastics are environmentally friendly.					
33278948	5	3	gly	heterogeneity	615:627	arg1	films					714:718	the films	710:718	the films	710:718	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	4	4	theme	agrowaste	552:560	arg1	beet					568:571	agrowaste sugar beet	552:571	agrowaste sugar beet pulp	552:576	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	3	5	theme	manufacturing	286:298	arg1	polysaccharides					327:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides	272:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides	272:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides can overcome such limitations.					
33278948	9	6	theme	new	1233:1235	arg1	lead					1237:1240	a new lead	1231:1240	a new lead in functional polysaccharide-based bioplastic systems	1231:1294	This study demonstrates a new lead in functional polysaccharide-based bioplastic systems.					
33278948	9	7	theme	polysaccharide-based	1256:1275	arg1	systems					1288:1294	functional polysaccharide-based bioplastic systems	1245:1294	functional polysaccharide-based bioplastic systems	1245:1294	This study demonstrates a new lead in functional polysaccharide-based bioplastic systems.					
33278948	9	8	theme	bioplastic	1277:1286	arg1	systems					1288:1294	functional polysaccharide-based bioplastic systems	1245:1294	functional polysaccharide-based bioplastic systems	1245:1294	This study demonstrates a new lead in functional polysaccharide-based bioplastic systems.					
33278948	5	9	theme	contact	693:699	arg1	angle					701:705	contact angle	693:705	contact angle	693:705	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	8	10	theme	anti-plasticizing	1016:1032	arg1	effects					1034:1040	Dynamic plasticizing and anti-plasticizing effects	991:1040	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol	991:1065	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol were demonstrated by NMR demonstrating high molecular order, but also non-crystalline, and evenly distributed 20 nm-sized glycerol domains.					
33278948	8	11	theme	plasticizing	999:1010	arg1	effects					1034:1040	Dynamic plasticizing and anti-plasticizing effects	991:1040	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol	991:1065	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol were demonstrated by NMR demonstrating high molecular order, but also non-crystalline, and evenly distributed 20 nm-sized glycerol domains.					
33278948	4	12	theme	bulk	463:466	arg1	quantity					468:475	bulk quantity	463:475	bulk quantity in transgenic barley grain	463:502	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	2	13	theme	typical	186:192	arg1	shortcomings					194:205	typical shortcomings	186:205	typical shortcomings	186:205	However, typical shortcomings include lack of water resistance and poor mechanical properties.					
33278948	5	14	theme	gas	661:663	arg1	permeability					675:686	gas and water permeability	661:686	permeability	675:686	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	9	15	theme	functional	1245:1254	arg1	systems					1288:1294	functional polysaccharide-based bioplastic systems	1245:1294	functional polysaccharide-based bioplastic systems	1245:1294	This study demonstrates a new lead in functional polysaccharide-based bioplastic systems.					
33278948	6	16	theme	Blending	739:746	arg1	CNF					748:750	Blending CNF	739:750	Blending CNF into the AM matrix	739:769	Blending CNF into the AM matrix significantly enhanced the crystallinity, mechanical properties and permeability, whereas glycerol increased elongation at break, mainly by plasticizing the AM.					
33278948	8	17	theme	high	1106:1109	arg1	order					1121:1125	high molecular order	1106:1125	high molecular order	1106:1125	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol were demonstrated by NMR demonstrating high molecular order, but also non-crystalline, and evenly distributed 20 nm-sized glycerol domains.					
33278948	5	18	theme	chemical	606:613	arg1	heterogeneity					615:627	chemical heterogeneity	606:627	chemical heterogeneity	606:627	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	0	19	theme	nanofiber	18:26	arg1	composites					28:37	Amylose/cellulose nanofiber composites	0:37	Amylose/cellulose nanofiber composites for all-natural, fully biodegradable and flexible bioplastics.	0:100	Amylose/cellulose nanofiber composites for all-natural, fully biodegradable and flexible bioplastics.					
33278948	3	20	theme	using	300:304	arg1	polysaccharides					327:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides	272:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides	272:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides can overcome such limitations.					
33278948	8	21	theme	CNF	1050:1052	arg1	effects					1034:1040	Dynamic plasticizing and anti-plasticizing effects	991:1040	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol	991:1065	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol were demonstrated by NMR demonstrating high molecular order, but also non-crystalline, and evenly distributed 20 nm-sized glycerol domains.					
33278948	1	22	theme	Thermoplastic	102:114	arg1	friendly					167:174	friendly	167:174	friendly	167:174	Thermoplastic, polysaccharide-based plastics are environmentally friendly.					
33278948	1	22	theme	Thermoplastic	102:114	arg1	plastics					138:145	Thermoplastic, polysaccharide-based plastics	102:145	Thermoplastic, polysaccharide-based plastics	102:145	Thermoplastic, polysaccharide-based plastics are environmentally friendly.					
33278948	0	23	theme	Amylose/cellulose	0:16	arg1	composites					28:37	Amylose/cellulose nanofiber composites	0:37	Amylose/cellulose nanofiber composites for all-natural, fully biodegradable and flexible bioplastics.	0:100	Amylose/cellulose nanofiber composites for all-natural, fully biodegradable and flexible bioplastics.					
33278948	3	24	theme	pure	306:309	arg1	polysaccharides					327:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides	272:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides	272:341	Nanocomposite manufacturing using pure, highly linear, polysaccharides can overcome such limitations.					
33278948	6	25	theme	mechanical	813:822	arg1	properties					824:833	mechanical properties	813:833	mechanical properties	813:833	Blending CNF into the AM matrix significantly enhanced the crystallinity, mechanical properties and permeability, whereas glycerol increased elongation at break, mainly by plasticizing the AM.					
33278948	8	26	theme	molecular	1111:1119	arg1	order					1121:1125	high molecular order	1106:1125	high molecular order	1106:1125	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol were demonstrated by NMR demonstrating high molecular order, but also non-crystalline, and evenly distributed 20 nm-sized glycerol domains.					
33278948	0	27	dep	all-natural	43:53	arg1	flexible					80:87	flexible	80:87	flexible	80:87	Amylose/cellulose nanofiber composites for all-natural, fully biodegradable and flexible bioplastics.					
33278948	0	27	dep	all-natural	43:53	arg1	biodegradable					62:74	biodegradable	62:74	biodegradable	62:74	Amylose/cellulose nanofiber composites for all-natural, fully biodegradable and flexible bioplastics.					
33278948	6	28	theme	AM	761:762	arg1	matrix					764:769	the AM matrix	757:769	the AM matrix	757:769	Blending CNF into the AM matrix significantly enhanced the crystallinity, mechanical properties and permeability, whereas glycerol increased elongation at break, mainly by plasticizing the AM.					
33278948	4	29	from	quantity	468:475	arg1	grain					498:502	transgenic barley grain	480:502	transgenic barley grain	480:502	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	2	30	theme	water	223:227	arg1	resistance					229:238	water resistance	223:238	water resistance	223:238	However, typical shortcomings include lack of water resistance and poor mechanical properties.					
33278948	9	31	from	lead	1237:1240	arg1	systems					1288:1294	functional polysaccharide-based bioplastic systems	1245:1294	functional polysaccharide-based bioplastic systems	1245:1294	This study demonstrates a new lead in functional polysaccharide-based bioplastic systems.					
33278948	4	32	dep	plant	415:419	arg1	AM					446:447	AM	446:447	AM	446:447	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	4	32	dep	plant	415:419	arg1	amylose					437:443	engineered pure amylose	421:443	engineered pure amylose (AM)	421:448	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	5	33	theme	water	669:673	arg1	permeability					675:686	gas and water permeability	661:686	permeability	675:686	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	0	34	theme	all-natural	43:53	arg1	bioplastics					89:99	all-natural, fully biodegradable and flexible bioplastics	43:99	all-natural, fully biodegradable and flexible bioplastics	43:99	Amylose/cellulose nanofiber composites for all-natural, fully biodegradable and flexible bioplastics.					
33278948	4	35	theme	beet	568:571	arg1	pulp					573:576	agrowaste sugar beet pulp	552:576	agrowaste sugar beet pulp	552:576	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	3	36	dep	pure	306:309	arg1	linear					319:324	linear	319:324	linear	319:324	Nanocomposite manufacturing using pure, highly linear, polysaccharides can overcome such limitations.					
33278948	4	37	theme	cellulose	509:517	arg1	CNF					531:533	CNF	531:533	CNF	531:533	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	4	37	theme	cellulose	509:517	arg1	nanofibers					519:528	cellulose nanofibers	509:528	cellulose nanofibers (CNF)	509:534	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	4	38	theme	sugar	562:566	arg1	beet					568:571	agrowaste sugar beet	552:571	agrowaste sugar beet pulp	552:576	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	2	39	theme	mechanical	249:258	arg1	properties					260:269	poor mechanical properties	244:269	poor mechanical properties	244:269	However, typical shortcomings include lack of water resistance and poor mechanical properties.					
33278948	2	40	theme	properties	260:269	arg1	lack					215:218	lack	215:218	lack of water resistance and poor mechanical properties	215:269	However, typical shortcomings include lack of water resistance and poor mechanical properties.					
33278948	4	41	theme	pure	432:435	arg1	AM					446:447	AM	446:447	AM	446:447	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	4	41	theme	pure	432:435	arg1	amylose					437:443	engineered pure amylose	421:443	engineered pure amylose (AM)	421:448	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	7	42	theme	phase	954:958	arg1	separation					960:969	significant phase separation	942:969	significant phase separation between AM and CNF	942:988	There was significant phase separation between AM and CNF.					
33278948	8	43	theme	glycerol	1058:1065	arg1	effects					1034:1040	Dynamic plasticizing and anti-plasticizing effects	991:1040	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol	991:1065	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol were demonstrated by NMR demonstrating high molecular order, but also non-crystalline, and evenly distributed 20 nm-sized glycerol domains.					
33278948	5	44	theme	films	714:718	arg1	mechanics					630:638	mechanics	630:638	mechanics	630:638	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	5	44	theme	films	714:718	arg1	Morphology					579:588	Morphology	579:588	Morphology	579:588	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	5	44	theme	films	714:718	arg1	permeability					675:686	gas and water permeability	661:686	permeability	675:686	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	5	44	theme	films	714:718	arg1	crystallinity					591:603	crystallinity	591:603	crystallinity	591:603	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	5	44	theme	films	714:718	arg1	heterogeneity					615:627	chemical heterogeneity	606:627	chemical heterogeneity	606:627	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	5	44	theme	films	714:718	arg1	angle					701:705	contact angle	693:705	contact angle	693:705	Morphology, crystallinity, chemical heterogeneity, mechanics, dynamic mechanical, gas and water permeability, and contact angle of the films were investigated.					
33278948	2	45	theme	poor	244:247	arg1	properties					260:269	poor mechanical properties	244:269	poor mechanical properties	244:269	However, typical shortcomings include lack of water resistance and poor mechanical properties.					
33278948	4	46	theme	engineered	421:430	arg1	AM					446:447	AM	446:447	AM	446:447	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	4	46	theme	engineered	421:430	arg1	amylose					437:443	engineered pure amylose	421:443	engineered pure amylose (AM)	421:448	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	3	47	theme	such	356:359	arg1	limitations					361:371	such limitations	356:371	such limitations	356:371	Nanocomposite manufacturing using pure, highly linear, polysaccharides can overcome such limitations.					
33278948	4	48	theme	barley	491:496	arg1	grain					498:502	transgenic barley grain	480:502	transgenic barley grain	480:502	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	8	49	theme	Dynamic	991:997	arg1	effects					1034:1040	Dynamic plasticizing and anti-plasticizing effects	991:1040	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol	991:1065	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol were demonstrated by NMR demonstrating high molecular order, but also non-crystalline, and evenly distributed 20 nm-sized glycerol domains.					
33278948	8	50	theme	glycerol	1189:1196	arg1	domains					1198:1204	20 nm-sized glycerol domains	1177:1204	20 nm-sized glycerol domains	1177:1204	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol were demonstrated by NMR demonstrating high molecular order, but also non-crystalline, and evenly distributed 20 nm-sized glycerol domains.					
33278948	2	51	theme	resistance	229:238	arg1	lack					215:218	lack	215:218	lack of water resistance and poor mechanical properties	215:269	However, typical shortcomings include lack of water resistance and poor mechanical properties.					
33278948	4	52	theme	Cast	374:377	arg1	nanocomposites					379:392	Cast nanocomposites	374:392	Cast nanocomposites	374:392	Cast nanocomposites were fabricated with plant engineered pure amylose (AM), produced in bulk quantity in transgenic barley grain, and cellulose nanofibers (CNF), extracted from agrowaste sugar beet pulp.					
33278948	7	53	theme	significant	942:952	arg1	separation					960:969	significant phase separation	942:969	significant phase separation between AM and CNF	942:988	There was significant phase separation between AM and CNF.					
33278948	8	54	theme	nm-sized	1180:1187	arg1	domains					1198:1204	20 nm-sized glycerol domains	1177:1204	20 nm-sized glycerol domains	1177:1204	Dynamic plasticizing and anti-plasticizing effects of both CNF and glycerol were demonstrated by NMR demonstrating high molecular order, but also non-crystalline, and evenly distributed 20 nm-sized glycerol domains.					
32059903	3	0	theme	enzymatic	294:302	arg1	purification					304:315	enzymatic purification	294:315	enzymatic purification	294:315	Acidic extraction was benchmarked against hot water extraction with enzymatic purification and against commercial ulvan.					
32059903	4	1	theme	colored	438:444	arg1	impurities					446:455	minimal colored impurities	430:455	minimal colored impurities	430:455	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	8	2	theme	common	981:986	arg1	K					1033:1033	K	1033:1033	K	1033:1033	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	2	theme	common	981:986	arg1	Ca					1017:1018	Ca	1017:1018	Ca	1017:1018	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	2	theme	common	981:986	arg1	elements					996:1003	common mineral elements	981:1003	common mineral elements such as Na, Ca, Mg, Al, and K	981:1033	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	2	theme	common	981:986	arg1	Mg					1021:1022	Mg	1021:1022	Mg	1021:1022	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	2	theme	common	981:986	arg1	Na					1013:1014	Na	1013:1014	Na	1013:1014	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	2	theme	common	981:986	arg1	Al					1025:1026	Al	1025:1026	Al	1025:1026	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	5	3	theme	molecular	478:486	arg1	660,000-760,000 g/mol					497:517	660,000-760,000 g/mol	497:517	660,000-760,000 g/mol	497:517	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	3	theme	molecular	478:486	arg1	weights					488:494	high molecular weights	473:494	high molecular weights (660,000-760,000 g/mol)	473:518	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	4	contain	had	469:471	arg1	ulvans					462:467	The ulvans	458:467	The ulvans	458:467	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	4	contain	had	469:471	arg2	660,000-760,000 g/mol					497:517	660,000-760,000 g/mol	497:517	660,000-760,000 g/mol	497:517	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	4	contain	had	469:471	arg2	weights					488:494	high molecular weights	473:494	high molecular weights (660,000-760,000 g/mol)	473:518	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	8	5	dep	metals	1058:1063	arg1	metals					1058:1063	the heavy metals Pb, Hg, or As	1048:1077	the heavy metals Pb, Hg, or As	1048:1077	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	5	dep	metals	1058:1063	arg1	Hg					1069:1070	Hg	1069:1070	Hg	1069:1070	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	5	dep	metals	1058:1063	arg1	As					1076:1077	As	1076:1077	As	1076:1077	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	5	dep	metals	1058:1063	arg1	Pb					1065:1066	Pb	1065:1066	Pb	1065:1066	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	6	theme	ash	922:924	arg1	analysis					910:917	Elemental analysis	900:917	Elemental analysis of ash remaining after thermal degradation	900:960	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	7	7	theme	isolated	810:817	arg1	ulvan					819:823	the isolated ulvan	806:823	the isolated ulvan	806:823	Acidic extraction caused almost complete desulfation of the isolated ulvan while the other method preserved a significant degree of SO3 substituents.					
32059903	8	8	theme	heavy	1052:1056	arg1	metals					1058:1063	the heavy metals Pb, Hg, or As	1048:1077	the heavy metals Pb, Hg, or As	1048:1077	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	8	theme	heavy	1052:1056	arg1	Hg					1069:1070	Hg	1069:1070	Hg	1069:1070	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	8	theme	heavy	1052:1056	arg1	Pb					1065:1066	Pb	1065:1066	Pb	1065:1066	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	9	theme	thermal	942:948	arg1	degradation					950:960	thermal degradation	942:960	thermal degradation	942:960	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	10	theme	elements	996:1003	arg1	presence					969:976	presence	969:976	presence of common mineral elements such as Na, Ca, Mg, Al, and K	969:1033	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	5	11	theme	%	547:547	arg1	acid					632:635	iduronic acid	623:635	iduronic acid	623:635	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	11	theme	%	547:547	arg1	rhamnose					575:582	rhamnose	575:582	rhamnose	575:582	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	11	theme	%	547:547	arg1	acid					613:616	glucuronic acid	602:616	glucuronic acid	602:616	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	11	theme	%	547:547	arg1	xylose					585:590	xylose	585:590	xylose	585:590	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	11	theme	%	547:547	arg1	carbohydrates					553:565	77-79 % g/g carbohydrates	541:565	77-79 % g/g carbohydrates	541:565	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	11	theme	%	547:547	arg1	glucose					593:599	glucose	593:599	glucose	593:599	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	6	12	theme	extraction	642:651	arg1	protocol					653:660	The extraction protocol	638:660	The extraction protocol	638:660	The extraction protocol and the ulvan source strongly impact the molecular weight and the chemical composition.					
32059903	4	13	dep	1.3	413:415	arg1	to					410:411	to	410:411	to	410:411	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	8	14	theme	mineral	988:994	arg1	K					1033:1033	K	1033:1033	K	1033:1033	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	14	theme	mineral	988:994	arg1	Ca					1017:1018	Ca	1017:1018	Ca	1017:1018	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	14	theme	mineral	988:994	arg1	elements					996:1003	common mineral elements	981:1003	common mineral elements such as Na, Ca, Mg, Al, and K	981:1033	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	14	theme	mineral	988:994	arg1	Mg					1021:1022	Mg	1021:1022	Mg	1021:1022	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	14	theme	mineral	988:994	arg1	Na					1013:1014	Na	1013:1014	Na	1013:1014	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	8	14	theme	mineral	988:994	arg1	Al					1025:1026	Al	1025:1026	Al	1025:1026	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	5	15	theme	g/g	549:551	arg1	acid					632:635	iduronic acid	623:635	iduronic acid	623:635	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	15	theme	g/g	549:551	arg1	rhamnose					575:582	rhamnose	575:582	rhamnose	575:582	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	15	theme	g/g	549:551	arg1	acid					613:616	glucuronic acid	602:616	glucuronic acid	602:616	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	15	theme	g/g	549:551	arg1	xylose					585:590	xylose	585:590	xylose	585:590	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	15	theme	g/g	549:551	arg1	carbohydrates					553:565	77-79 % g/g carbohydrates	541:565	77-79 % g/g carbohydrates	541:565	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	15	theme	g/g	549:551	arg1	glucose					593:599	glucose	593:599	glucose	593:599	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	7	16	theme	Acidic	750:755	arg1	extraction					757:766	Acidic extraction	750:766	Acidic extraction	750:766	Acidic extraction caused almost complete desulfation of the isolated ulvan while the other method preserved a significant degree of SO3 substituents.					
32059903	3	17	theme	commercial	329:338	arg1	ulvan					340:344	commercial ulvan	329:344	commercial ulvan	329:344	Acidic extraction was benchmarked against hot water extraction with enzymatic purification and against commercial ulvan.					
32059903	4	18	theme	ash	388:390	arg1	g/g					381:383	11-18 % g/g	373:383	11-18 % g/g of ash, some protein (up to 1.3 % g N/g)	373:424	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	2	19	theme	polysaccharide	115:128	arg1	ulvan					130:134	The cell wall polysaccharide ulvan	101:134	The cell wall polysaccharide ulvan	101:134	The cell wall polysaccharide ulvan was isolated from two species of the seaweed Ulva collected along the Swedish west coast.					
32059903	3	20	theme	Acidic	226:231	arg1	extraction					233:242	Acidic extraction	226:242	Acidic extraction	226:242	Acidic extraction was benchmarked against hot water extraction with enzymatic purification and against commercial ulvan.					
32059903	7	21	theme	complete	782:789	arg1	desulfation					791:801	almost complete desulfation	775:801	almost complete desulfation of the isolated ulvan	775:823	Acidic extraction caused almost complete desulfation of the isolated ulvan while the other method preserved a significant degree of SO3 substituents.					
32059903	7	22	theme	substituents	886:897	arg1	degree					872:877	a significant degree	858:877	a significant degree of SO3 substituents	858:897	Acidic extraction caused almost complete desulfation of the isolated ulvan while the other method preserved a significant degree of SO3 substituents.					
32059903	2	23	theme	wall	110:113	arg1	ulvan					130:134	The cell wall polysaccharide ulvan	101:134	The cell wall polysaccharide ulvan	101:134	The cell wall polysaccharide ulvan was isolated from two species of the seaweed Ulva collected along the Swedish west coast.					
32059903	7	24	theme	SO3	882:884	arg1	substituents					886:897	SO3 substituents	882:897	SO3 substituents	882:897	Acidic extraction caused almost complete desulfation of the isolated ulvan while the other method preserved a significant degree of SO3 substituents.					
32059903	3	25	with	extraction	278:287	arg1	purification					304:315	enzymatic purification	294:315	enzymatic purification	294:315	Acidic extraction was benchmarked against hot water extraction with enzymatic purification and against commercial ulvan.					
32059903	4	26	theme	%	379:379	arg1	g/g					381:383	11-18 % g/g	373:383	11-18 % g/g of ash, some protein (up to 1.3 % g N/g)	373:424	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	8	27	theme	Elemental	900:908	arg1	analysis					910:917	Elemental analysis	900:917	Elemental analysis of ash remaining after thermal degradation	900:960	Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.					
32059903	6	28	theme	molecular	703:711	arg1	weight					713:718	the molecular weight	699:718	the molecular weight	699:718	The extraction protocol and the ulvan source strongly impact the molecular weight and the chemical composition.					
32059903	5	29	theme	iduronic	623:630	arg1	acid					632:635	iduronic acid	623:635	iduronic acid	623:635	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	29	theme	iduronic	623:630	arg1	carbohydrates					553:565	77-79 % g/g carbohydrates	541:565	77-79 % g/g carbohydrates	541:565	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	4	30	theme	11-18	373:377	arg1	%					379:379	%	379:379	%	379:379	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	1	31	theme	Swedish	81:87	arg1	coast					94:98	the Swedish west coast	77:98	the Swedish west coast	77:98	along the Swedish west coast.					
32059903	4	32	contain	contained	363:371	arg1	ulvan					357:361	Extracted ulvan	347:361	Extracted ulvan	347:361	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	4	32	contain	contained	363:371	arg2	g/g					381:383	11-18 % g/g	373:383	11-18 % g/g of ash, some protein (up to 1.3 % g N/g)	373:424	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	4	32	contain	contained	363:371	arg2	impurities					446:455	minimal colored impurities	430:455	minimal colored impurities	430:455	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	1	33	theme	west	89:92	arg1	coast					94:98	the Swedish west coast	77:98	the Swedish west coast	77:98	along the Swedish west coast.					
32059903	6	34	theme	chemical	728:735	arg1	composition					737:747	the chemical composition	724:747	the chemical composition	724:747	The extraction protocol and the ulvan source strongly impact the molecular weight and the chemical composition.					
32059903	0	35	theme	wall	34:37	arg1	ulvans					39:44	cell wall ulvans	29:44	cell wall ulvans	29:44	Composition and structure of cell wall ulvans recovered from Ulva spp.					
32059903	2	36	theme	Swedish	206:212	arg1	coast					219:223	the Swedish west coast	202:223	the Swedish west coast	202:223	The cell wall polysaccharide ulvan was isolated from two species of the seaweed Ulva collected along the Swedish west coast.					
32059903	2	37	attach	isolated	140:147	arg2	ulvan					130:134	The cell wall polysaccharide ulvan	101:134	The cell wall polysaccharide ulvan	101:134	The cell wall polysaccharide ulvan was isolated from two species of the seaweed Ulva collected along the Swedish west coast.					
32059903	2	37	attach	isolated	140:147	arg1	species					158:164	two species	154:164	two species of the seaweed Ulva collected along the Swedish west coast	154:223	The cell wall polysaccharide ulvan was isolated from two species of the seaweed Ulva collected along the Swedish west coast.					
32059903	0	38	theme	cell	29:32	arg1	ulvans					39:44	cell wall ulvans	29:44	cell wall ulvans	29:44	Composition and structure of cell wall ulvans recovered from Ulva spp.					
32059903	4	39	theme	g	419:419	arg1	N/g					421:423	up to 1.3 % g N/g	407:423	up to 1.3 % g N/g	407:423	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	4	39	theme	g	419:419	arg1	protein					398:404	some protein	393:404	some protein (up to 1.3 % g N/g)	393:424	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	5	40	theme	glucuronic	602:611	arg1	acid					613:616	glucuronic acid	602:616	glucuronic acid	602:616	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	40	theme	glucuronic	602:611	arg1	carbohydrates					553:565	77-79 % g/g carbohydrates	541:565	77-79 % g/g carbohydrates	541:565	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	4	41	theme	%	417:417	arg1	N/g					421:423	up to 1.3 % g N/g	407:423	up to 1.3 % g N/g	407:423	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	4	41	theme	%	417:417	arg1	protein					398:404	some protein	393:404	some protein (up to 1.3 % g N/g)	393:424	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	4	42	theme	minimal	430:436	arg1	impurities					446:455	minimal colored impurities	430:455	minimal colored impurities	430:455	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	0	43	theme	ulvans	39:44	arg1	structure					16:24	structure	16:24	structure	16:24	Composition and structure of cell wall ulvans recovered from Ulva spp.					
32059903	0	43	theme	ulvans	39:44	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and structure of cell wall ulvans recovered from Ulva spp.					
32059903	2	44	theme	Ulva	181:184	arg1	species					158:164	two species	154:164	two species of the seaweed Ulva collected along the Swedish west coast	154:223	The cell wall polysaccharide ulvan was isolated from two species of the seaweed Ulva collected along the Swedish west coast.					
32059903	4	45	theme	Extracted	347:355	arg1	ulvan					357:361	Extracted ulvan	347:361	Extracted ulvan	347:361	Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities.					
32059903	3	46	theme	hot	268:270	arg1	extraction					278:287	hot water extraction	268:287	hot water extraction with enzymatic purification	268:315	Acidic extraction was benchmarked against hot water extraction with enzymatic purification and against commercial ulvan.					
32059903	6	47	theme	ulvan	670:674	arg1	source					676:681	the ulvan source	666:681	the ulvan source	666:681	The extraction protocol and the ulvan source strongly impact the molecular weight and the chemical composition.					
32059903	3	48	theme	water	272:276	arg1	extraction					278:287	hot water extraction	268:287	hot water extraction with enzymatic purification	268:315	Acidic extraction was benchmarked against hot water extraction with enzymatic purification and against commercial ulvan.					
32059903	7	49	theme	other	835:839	arg1	method					841:846	the other method	831:846	the other method	831:846	Acidic extraction caused almost complete desulfation of the isolated ulvan while the other method preserved a significant degree of SO3 substituents.					
32059903	7	50	theme	ulvan	819:823	arg1	desulfation					791:801	almost complete desulfation	775:801	almost complete desulfation of the isolated ulvan	775:823	Acidic extraction caused almost complete desulfation of the isolated ulvan while the other method preserved a significant degree of SO3 substituents.					
32059903	2	51	theme	cell	105:108	arg1	ulvan					130:134	The cell wall polysaccharide ulvan	101:134	The cell wall polysaccharide ulvan	101:134	The cell wall polysaccharide ulvan was isolated from two species of the seaweed Ulva collected along the Swedish west coast.					
32059903	2	52	theme	west	214:217	arg1	coast					219:223	the Swedish west coast	202:223	the Swedish west coast	202:223	The cell wall polysaccharide ulvan was isolated from two species of the seaweed Ulva collected along the Swedish west coast.					
32059903	5	53	theme	high	473:476	arg1	660,000-760,000 g/mol					497:517	660,000-760,000 g/mol	497:517	660,000-760,000 g/mol	497:517	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	5	53	theme	high	473:476	arg1	weights					488:494	high molecular weights	473:494	high molecular weights (660,000-760,000 g/mol)	473:518	The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid.					
32059903	7	54	theme	significant	860:870	arg1	degree					872:877	a significant degree	858:877	a significant degree of SO3 substituents	858:897	Acidic extraction caused almost complete desulfation of the isolated ulvan while the other method preserved a significant degree of SO3 substituents.					
34161137	4	0	theme	glycoproteins	691:703	arg1	stability					678:686	the stability	674:686	the stability of glycoproteins and Golgi enzymes	674:721	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	1	1	theme	Golgi	140:144	arg1	hub					167:169	a central hub	157:169	a central hub for intracellular protein trafficking and glycosylation	157:225	The Golgi complex is a central hub for intracellular protein trafficking and glycosylation.					
34161137	1	1	theme	Golgi	140:144	arg1	complex					146:152	The Golgi complex	136:152	The Golgi complex	136:152	The Golgi complex is a central hub for intracellular protein trafficking and glycosylation.					
34161137	0	2	theme	essential	64:72	arg1	role					74:77	an essential role	61:77	an essential role	61:77	The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery.					
34161137	7	3	theme	machinery	1219:1227	arg1	recycling					1164:1172	recycling	1164:1172	recycling	1164:1172	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	7	3	theme	machinery	1219:1227	arg1	stabilization					1178:1190	stabilization	1178:1190	stabilization	1178:1190	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	5	4	from	retention	775:783	arg1	Golgi					817:821	the Golgi	813:821	the Golgi	813:821	Moreover, GARP-knockout (KO) cells exhibit reduced retention of glycosylation enzymes in the Golgi.					
34161137	7	5	theme	Golgi	1079:1083	arg1	enzymes					1085:1091	Golgi enzymes	1079:1091	Golgi enzymes	1079:1091	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	4	6	theme	Golgi	623:627	arg1	modification					629:640	Golgi modification	623:640	Golgi modification of N- and O-glycans	623:660	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	7	7	theme	enzymes	1085:1091	arg1	itinerary					1066:1074	the trafficking itinerary	1050:1074	the trafficking itinerary of Golgi enzymes or their recycling adaptors	1050:1119	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	3	8	theme	critical	504:511	arg1	component					513:521	a critical component	502:521	a critical component of this machinery	502:539	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	3	8	theme	critical	504:511	arg1	complex					491:497	the Golgi-associated retrograde protein (GARP) complex	444:497	the Golgi-associated retrograde protein (GARP) complex	444:497	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	4	9	theme	N-	645:646	arg1	modification					629:640	Golgi modification	623:640	Golgi modification of N- and O-glycans	623:660	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	5	10	theme	GARP-knockout	734:746	arg1	cells					753:757	GARP-knockout (KO) cells	734:757	GARP-knockout (KO) cells	734:757	Moreover, GARP-knockout (KO) cells exhibit reduced retention of glycosylation enzymes in the Golgi.					
34161137	7	11	theme	GARP	1134:1137	arg1	essential					1150:1158	essential	1150:1158	essential	1150:1158	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	7	11	theme	GARP	1134:1137	arg1	complex					1139:1145	the GARP complex	1130:1145	the GARP complex	1130:1145	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	3	12	theme	GARP	485:488	arg1	component					513:521	a critical component	502:521	a critical component of this machinery	502:539	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	3	12	theme	GARP	485:488	arg1	complex					491:497	the Golgi-associated retrograde protein (GARP) complex	444:497	the Golgi-associated retrograde protein (GARP) complex	444:497	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	3	13	theme	machinery	531:539	arg1	component					513:521	a critical component	502:521	a critical component of this machinery	502:539	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	3	13	theme	machinery	531:539	arg1	complex					491:497	the Golgi-associated retrograde protein (GARP) complex	444:497	the Golgi-associated retrograde protein (GARP) complex	444:497	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	4	14	theme	O-glycans	652:660	arg1	modification					629:640	Golgi modification	623:640	Golgi modification of N- and O-glycans	623:660	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	7	15	theme	adaptors	1112:1119	arg1	itinerary					1066:1074	the trafficking itinerary	1050:1074	the trafficking itinerary of Golgi enzymes or their recycling adaptors	1050:1119	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	5	16	theme	reduced	767:773	arg1	retention					775:783	reduced retention	767:783	reduced retention of glycosylation enzymes in the Golgi	767:821	Moreover, GARP-knockout (KO) cells exhibit reduced retention of glycosylation enzymes in the Golgi.					
34161137	3	17	theme	Golgi-associated	448:463	arg1	component					513:521	a critical component	502:521	a critical component of this machinery	502:539	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	3	17	theme	Golgi-associated	448:463	arg1	complex					491:497	the Golgi-associated retrograde protein (GARP) complex	444:497	the Golgi-associated retrograde protein (GARP) complex	444:497	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	0	18	theme	Golgi-associated	4:19	arg1	complex					47:53	The Golgi-associated retrograde protein (GARP) complex	0:53	The Golgi-associated retrograde protein (GARP) complex	0:53	The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery.					
34161137	6	19	theme	GARP-KO	852:858	arg1	cells					860:864	GARP-KO cells	852:864	GARP-KO cells	852:864	A RUSH assay shows that, in GARP-KO cells, the enzyme beta-1,4-galactosyltransferase 1 is not retained at the Golgi complex but instead is missorted to the endolysosomal system.					
34161137	8	20	dep	[Media	1230:1235	arg1	see					1238:1240	see	1238:1240	see text	1238:1245	[Media: see text].					
34161137	4	21	mod	modification	629:640	arg3	Golgi					623:627	Golgi modification	623:640	Golgi modification of N- and O-glycans	623:660	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	4	21	mod	modification	629:640	arg1	O-glycans					652:660	O-glycans	652:660	O-glycans	652:660	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	4	21	mod	modification	629:640	arg1	N-					645:646	N-	645:646	N-	645:646	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	1	22	theme	central	159:165	arg1	hub					167:169	a central hub	157:169	a central hub for intracellular protein trafficking and glycosylation	157:225	The Golgi complex is a central hub for intracellular protein trafficking and glycosylation.					
34161137	1	22	theme	central	159:165	arg1	complex					146:152	The Golgi complex	136:152	The Golgi complex	136:152	The Golgi complex is a central hub for intracellular protein trafficking and glycosylation.					
34161137	4	23	theme	cell	563:566	arg1	lines					568:572	multiple human cell lines	548:572	multiple human cell lines	548:572	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	7	24	theme	trafficking	1054:1064	arg1	itinerary					1066:1074	the trafficking itinerary	1050:1074	the trafficking itinerary of Golgi enzymes or their recycling adaptors	1050:1119	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	2	25	theme	mechanisms	311:320	arg1	combination					296:306	a combination	294:306	a combination of mechanisms involving retention and recycling	294:354	Steady-state localization of glycosylation enzymes is achieved by a combination of mechanisms involving retention and recycling, but the machinery governing these mechanisms is poorly understood.					
34161137	0	26	theme	protein	32:38	arg1	complex					47:53	The Golgi-associated retrograde protein (GARP) complex	0:53	The Golgi-associated retrograde protein (GARP) complex	0:53	The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery.					
34161137	4	27	theme	human	557:561	arg1	lines					568:572	multiple human cell lines	548:572	multiple human cell lines	548:572	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	0	28	theme	Golgi	105:109	arg1	machinery					125:133	the Golgi glycosylation machinery	101:133	the Golgi glycosylation machinery	101:133	The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery.					
34161137	0	29	theme	retrograde	21:30	arg1	complex					47:53	The Golgi-associated retrograde protein (GARP) complex	0:53	The Golgi-associated retrograde protein (GARP) complex	0:53	The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery.					
34161137	1	30	theme	intracellular	175:187	arg1	trafficking					197:207	intracellular protein trafficking	175:207	intracellular protein trafficking	175:207	The Golgi complex is a central hub for intracellular protein trafficking and glycosylation.					
34161137	0	31	theme	GARP	41:44	arg1	complex					47:53	The Golgi-associated retrograde protein (GARP) complex	0:53	The Golgi-associated retrograde protein (GARP) complex	0:53	The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery.					
34161137	6	32	theme	Golgi	934:938	arg1	complex					940:946	the Golgi complex	930:946	the Golgi complex	930:946	A RUSH assay shows that, in GARP-KO cells, the enzyme beta-1,4-galactosyltransferase 1 is not retained at the Golgi complex but instead is missorted to the endolysosomal system.					
34161137	0	33	theme	machinery	125:133	arg1	maintenance					86:96	the maintenance	82:96	the maintenance of the Golgi glycosylation machinery	82:133	The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery.					
34161137	1	34	theme	protein	189:195	arg1	trafficking					197:207	intracellular protein trafficking	175:207	intracellular protein trafficking	175:207	The Golgi complex is a central hub for intracellular protein trafficking and glycosylation.					
34161137	2	35	theme	Steady-state	228:239	arg1	localization					241:252	Steady-state localization	228:252	Steady-state localization of glycosylation enzymes	228:277	Steady-state localization of glycosylation enzymes is achieved by a combination of mechanisms involving retention and recycling, but the machinery governing these mechanisms is poorly understood.					
34161137	3	36	theme	retrograde	465:474	arg1	component					513:521	a critical component	502:521	a critical component of this machinery	502:539	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	3	36	theme	retrograde	465:474	arg1	complex					491:497	the Golgi-associated retrograde protein (GARP) complex	444:497	the Golgi-associated retrograde protein (GARP) complex	444:497	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	0	37	theme	glycosylation	111:123	arg1	machinery					125:133	the Golgi glycosylation machinery	101:133	the Golgi glycosylation machinery	101:133	The Golgi-associated retrograde protein (GARP) complex plays an essential role in the maintenance of the Golgi glycosylation machinery.					
34161137	3	38	theme	protein	476:482	arg1	component					513:521	a critical component	502:521	a critical component of this machinery	502:539	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	3	38	theme	protein	476:482	arg1	complex					491:497	the Golgi-associated retrograde protein (GARP) complex	444:497	the Golgi-associated retrograde protein (GARP) complex	444:497	Herein we show that the Golgi-associated retrograde protein (GARP) complex is a critical component of this machinery.					
34161137	7	39	theme	recycling	1102:1110	arg1	adaptors					1112:1119	their recycling adaptors	1096:1119	their recycling adaptors	1096:1119	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	4	40	theme	Golgi	709:713	arg1	enzymes					715:721	Golgi enzymes	709:721	Golgi enzymes	709:721	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	5	41	theme	glycosylation	788:800	arg1	enzymes					802:808	glycosylation enzymes	788:808	glycosylation enzymes	788:808	Moreover, GARP-knockout (KO) cells exhibit reduced retention of glycosylation enzymes in the Golgi.					
34161137	4	42	theme	multiple	548:555	arg1	lines					568:572	multiple human cell lines	548:572	multiple human cell lines	548:572	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	7	43	theme	endosomal	1022:1030	arg1	part					1042:1045	part	1042:1045	part of the trafficking itinerary of Golgi enzymes or their recycling adaptors	1042:1119	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	7	43	theme	endosomal	1022:1030	arg1	system					1032:1037	the endosomal system	1018:1037	the endosomal system	1018:1037	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	6	44	theme	RUSH	826:829	arg1	assay					831:835	A RUSH assay	824:835	A RUSH assay	824:835	A RUSH assay shows that, in GARP-KO cells, the enzyme beta-1,4-galactosyltransferase 1 is not retained at the Golgi complex but instead is missorted to the endolysosomal system.					
34161137	2	45	theme	enzymes	271:277	arg1	localization					241:252	Steady-state localization	228:252	Steady-state localization of glycosylation enzymes	228:277	Steady-state localization of glycosylation enzymes is achieved by a combination of mechanisms involving retention and recycling, but the machinery governing these mechanisms is poorly understood.					
34161137	7	46	theme	itinerary	1066:1074	arg1	part					1042:1045	part	1042:1045	part of the trafficking itinerary of Golgi enzymes or their recycling adaptors	1042:1119	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	7	46	theme	itinerary	1066:1074	arg1	system					1032:1037	the endosomal system	1018:1037	the endosomal system	1018:1037	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	4	47	theme	GARP	601:604	arg1	subunits					606:613	GARP subunits	601:613	GARP subunits	601:613	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	4	48	gly	glycoproteins	691:703	arg1	glycoproteins					691:703	glycoproteins	691:703	glycoproteins	691:703	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	2	49	theme	glycosylation	257:269	arg1	enzymes					271:277	glycosylation enzymes	257:277	glycosylation enzymes	257:277	Steady-state localization of glycosylation enzymes is achieved by a combination of mechanisms involving retention and recycling, but the machinery governing these mechanisms is poorly understood.					
34161137	4	50	theme	enzymes	715:721	arg1	stability					678:686	the stability	674:686	the stability of glycoproteins and Golgi enzymes	674:721	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
34161137	5	51	theme	KO	749:750	arg1	cells					753:757	GARP-knockout (KO) cells	734:757	GARP-knockout (KO) cells	734:757	Moreover, GARP-knockout (KO) cells exhibit reduced retention of glycosylation enzymes in the Golgi.					
34161137	6	52	theme	enzyme	871:876	arg1	beta-1,4-galactosyltransferase					878:907	the enzyme beta-1,4-galactosyltransferase 1	867:909	the enzyme beta-1,4-galactosyltransferase 1	867:909	A RUSH assay shows that, in GARP-KO cells, the enzyme beta-1,4-galactosyltransferase 1 is not retained at the Golgi complex but instead is missorted to the endolysosomal system.					
34161137	7	53	theme	Golgi	1199:1203	arg1	machinery					1219:1227	the Golgi glycosylation machinery	1195:1227	the Golgi glycosylation machinery	1195:1227	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	6	54	theme	endolysosomal	980:992	arg1	system					994:999	the endolysosomal system	976:999	the endolysosomal system	976:999	A RUSH assay shows that, in GARP-KO cells, the enzyme beta-1,4-galactosyltransferase 1 is not retained at the Golgi complex but instead is missorted to the endolysosomal system.					
34161137	5	55	theme	enzymes	802:808	arg1	retention					775:783	reduced retention	767:783	reduced retention of glycosylation enzymes in the Golgi	767:821	Moreover, GARP-knockout (KO) cells exhibit reduced retention of glycosylation enzymes in the Golgi.					
34161137	7	56	theme	glycosylation	1205:1217	arg1	machinery					1219:1227	the Golgi glycosylation machinery	1195:1227	the Golgi glycosylation machinery	1195:1227	We propose that the endosomal system is part of the trafficking itinerary of Golgi enzymes or their recycling adaptors and that the GARP complex is essential for recycling and stabilization of the Golgi glycosylation machinery.					
34161137	4	57	theme	subunits	606:613	arg1	depletion					588:596	depletion	588:596	depletion of GARP subunits	588:613	Using multiple human cell lines, we show that depletion of GARP subunits impairs Golgi modification of N- and O-glycans and reduces the stability of glycoproteins and Golgi enzymes.					
33915214	5	0	with	spectroscopy	918:929	arg1	reflectance					874:884	attenuated total reflectance	857:884	attenuated total reflectance	857:884	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	3	1	theme	anionic	666:672	arg1	CAR					692:694	CAR	692:694	CAR	692:694	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	3	1	theme	anionic	666:672	arg1	iota-carrageenan					674:689	the anionic iota-carrageenan	662:689	the anionic iota-carrageenan (CAR)	662:695	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	9	2	theme	food	1542:1545	arg1	properties					1478:1487	properties	1478:1487	properties that can be further developed as wound dressings and food packaging	1478:1555	Therefore, they have properties that can be further developed as wound dressings and food packaging.					
33915214	9	2	theme	food	1542:1545	arg1	packaging					1547:1555	food packaging	1542:1555	food packaging	1542:1555	Therefore, they have properties that can be further developed as wound dressings and food packaging.					
33915214	6	3	theme	lowest	1182:1187	arg1	nm					1213:1214	Rq = 97 ± 12 nm	1200:1214	Rq = 97 ± 12 nm	1200:1214	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	3	theme	lowest	1182:1187	arg1	roughness					1189:1197	the lowest roughness	1178:1197	the lowest roughness (Rq = 97 ± 12 nm)	1178:1215	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	9	4	theme	wound	1522:1526	arg1	dressings					1528:1536	wound dressings	1522:1536	wound dressings	1522:1536	Therefore, they have properties that can be further developed as wound dressings and food packaging.					
33915214	9	4	theme	wound	1522:1526	arg1	properties					1478:1487	properties	1478:1487	properties that can be further developed as wound dressings and food packaging	1478:1555	Therefore, they have properties that can be further developed as wound dressings and food packaging.					
33915214	0	5	theme	chitosan	59:66	arg1	multilayers					103:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers	0:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.	0:134	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	5	6	theme	contact	979:985	arg1	WCA					994:996	WCA	994:996	WCA	994:996	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	6	theme	contact	979:985	arg1	angle					987:991	water contact angle	973:991	water contact angle (WCA) measurements	973:1010	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	6	7	theme	TMC/CAR	1114:1120	arg1	coating					1122:1128	the TMC/CAR coating	1110:1128	the TMC/CAR coating	1110:1128	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	2	8	contain	have	423:426	arg1	PEMs					333:336	PEMs	333:336	PEMs composed of iota-carrageenan (CAR)	333:371	We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth.					
33915214	2	8	contain	have	423:426	arg2	properties					464:473	composition-dependent antimicrobial properties	428:473	composition-dependent antimicrobial properties	428:473	We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth.					
33915214	2	8	contain	have	423:426	arg1	polymers					414:421	three different natural polycationic polymers	377:421	three different natural polycationic polymers	377:421	We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth.					
33915214	0	9	theme	tanfloc-based	73:85	arg1	polyelectrolyte					87:101	tanfloc-based polyelectrolyte	73:101	tanfloc-based polyelectrolyte	73:101	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	6	10	theme	=	1090:1090	arg1	surface					1078:1084	a hydrophobic (WCA = 127°) and rough surface	1041:1084	a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm)	1041:1103	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	10	theme	=	1090:1090	arg1	nm					1101:1102	Rq = 243 ± 48 nm	1087:1102	Rq = 243 ± 48 nm	1087:1102	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	7	11	theme	Polymer	1218:1224	arg1	coatings					1226:1233	Polymer coatings	1218:1233	Polymer coatings	1218:1233	Polymer coatings promote stability and durability of the GG film, and introduce antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria.					
33915214	5	12	theme	total	868:872	arg1	reflectance					874:884	attenuated total reflectance	857:884	attenuated total reflectance	857:884	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	6	13	theme	hydrophobic	1043:1053	arg1	surface					1078:1084	a hydrophobic (WCA = 127°) and rough surface	1041:1084	a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm)	1041:1103	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	13	theme	hydrophobic	1043:1053	arg1	nm					1101:1102	Rq = 243 ± 48 nm	1087:1102	Rq = 243 ± 48 nm	1087:1102	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	1	14	theme	polysaccharide-based	235:254	arg1	multilayers					272:282	polysaccharide-based polyelectrolyte multilayers	235:282	polysaccharide-based polyelectrolyte multilayers	235:282	In this work free-standing gels formed from gellan gum (GG) by solvent evaporation are coated with polysaccharide-based polyelectrolyte multilayers, using the layer-by-layer approach.					
33915214	7	15	dep	bacteria	1412:1419	arg1	aureus					1404:1409	Staphylococcus aureus	1389:1409	Staphylococcus aureus	1389:1409	Polymer coatings promote stability and durability of the GG film, and introduce antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria.					
33915214	5	16	theme	GG	771:772	arg1	film					774:777	The GG film and coated GG films	767:797	film	774:777	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	17	theme	X-ray	898:902	arg1	XPS					932:934	XPS	932:934	XPS	932:934	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	17	theme	X-ray	898:902	arg1	spectroscopy					918:929	X-ray photoelectron spectroscopy	898:929	X-ray photoelectron spectroscopy (XPS)	898:935	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	1	18	theme	free-standing	149:161	arg1	gels					163:166	free-standing gels	149:166	free-standing gels formed from gellan gum (GG) by solvent evaporation	149:217	In this work free-standing gels formed from gellan gum (GG) by solvent evaporation are coated with polysaccharide-based polyelectrolyte multilayers, using the layer-by-layer approach.					
33915214	0	19	theme	polyelectrolyte	87:101	arg1	multilayers					103:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers	0:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.	0:134	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	0	20	theme	N	43:43	arg1	multilayers					103:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers	0:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.	0:134	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	2	21	theme	antimicrobial	450:462	arg1	properties					464:473	composition-dependent antimicrobial properties	428:473	composition-dependent antimicrobial properties	428:473	We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth.					
33915214	5	22	theme	GG	790:791	arg1	films					793:797	The GG film and coated GG films	767:797	films	793:797	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	2	23	theme	cell	498:501	arg1	growth					503:508	mammalian cell growth	488:508	mammalian cell growth	488:508	We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth.					
33915214	6	24	theme	=	1203:1203	arg1	nm					1213:1214	Rq = 97 ± 12 nm	1200:1214	Rq = 97 ± 12 nm	1200:1214	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	24	theme	=	1203:1203	arg1	roughness					1189:1197	the lowest roughness	1178:1197	the lowest roughness (Rq = 97 ± 12 nm)	1178:1215	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	3	25	dep	polymers	520:527	arg1	chitosan					530:537	chitosan (CHT)	530:543	chitosan (CHT)	530:543	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	6	26	theme	hydrophilic	1141:1151	arg1	surface					1153:1159	a hydrophilic surface	1139:1159	a hydrophilic surface (WCA = 78°)	1139:1171	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	26	theme	hydrophilic	1141:1151	arg1	78°					1168:1170	78°	1168:1170	78°	1168:1170	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	27	theme	±	1208:1208	arg1	nm					1213:1214	Rq = 97 ± 12 nm	1200:1214	Rq = 97 ± 12 nm	1200:1214	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	27	theme	±	1208:1208	arg1	roughness					1189:1197	the lowest roughness	1178:1197	the lowest roughness (Rq = 97 ± 12 nm)	1178:1215	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	0	28	theme	Antimicrobial	0:12	arg1	multilayers					103:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers	0:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.	0:134	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	1	29	theme	polyelectrolyte	256:270	arg1	multilayers					272:282	polysaccharide-based polyelectrolyte multilayers	235:282	polysaccharide-based polyelectrolyte multilayers	235:282	In this work free-standing gels formed from gellan gum (GG) by solvent evaporation are coated with polysaccharide-based polyelectrolyte multilayers, using the layer-by-layer approach.					
33915214	5	30	with	spectroscopy	839:850	arg1	reflectance					874:884	attenuated total reflectance	857:884	attenuated total reflectance	857:884	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	7	31	theme	film	1278:1281	arg1	durability					1257:1266	durability	1257:1266	durability	1257:1266	Polymer coatings promote stability and durability of the GG film, and introduce antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria.					
33915214	7	31	theme	film	1278:1281	arg1	stability					1243:1251	stability	1243:1251	stability	1243:1251	Polymer coatings promote stability and durability of the GG film, and introduce antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria.					
33915214	3	32	theme	N-trimethyl	550:560	arg1	TMC					572:574	TMC	572:574	TMC	572:574	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	3	32	theme	N-trimethyl	550:560	arg1	chitosan					562:569	N-trimethyl chitosan	550:569	N-trimethyl chitosan (TMC)	550:575	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	6	33	dep	hydrophobic	1043:1053	arg1	WCA					1056:1058	WCA	1056:1058	WCA = 127°	1056:1065	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	2	34	theme	composition-dependent	428:448	arg1	properties					464:473	composition-dependent antimicrobial properties	428:473	composition-dependent antimicrobial properties	428:473	We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth.					
33915214	0	35	theme	gellan	118:123	arg1	films					129:133	gellan gum films	118:133	gellan gum films	118:133	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	3	36	dep	chitosan	530:537	arg1	TMC					572:574	TMC	572:574	TMC	572:574	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	3	36	dep	chitosan	530:537	arg1	derivative					613:622	an amino-functionalized tannin derivative	582:622	an amino-functionalized tannin derivative (TN)	582:627	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	3	36	dep	chitosan	530:537	arg1	N					548:548	N	548:548	N	548:548	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	3	36	dep	chitosan	530:537	arg1	TN					625:626	TN	625:626	TN	625:626	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	3	36	dep	chitosan	530:537	arg1	chitosan					562:569	N-trimethyl chitosan	550:569	N-trimethyl chitosan (TMC)	550:575	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	3	36	dep	chitosan	530:537	arg1	N					546:546	N	546:546	N	546:546	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	6	37	theme	±	1096:1096	arg1	surface					1078:1084	a hydrophobic (WCA = 127°) and rough surface	1041:1084	a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm)	1041:1103	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	37	theme	±	1096:1096	arg1	nm					1101:1102	Rq = 243 ± 48 nm	1087:1102	Rq = 243 ± 48 nm	1087:1102	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	1	38	theme	gellan	180:185	arg1	gum					187:189	gellan gum	180:189	gellan gum (GG)	180:194	In this work free-standing gels formed from gellan gum (GG) by solvent evaporation are coated with polysaccharide-based polyelectrolyte multilayers, using the layer-by-layer approach.					
33915214	1	38	theme	gellan	180:185	arg1	GG					192:193	GG	192:193	GG	192:193	In this work free-standing gels formed from gellan gum (GG) by solvent evaporation are coated with polysaccharide-based polyelectrolyte multilayers, using the layer-by-layer approach.					
33915214	0	39	theme	cytocompatible	18:31	arg1	multilayers					103:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers	0:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.	0:134	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	6	40	theme	=	1060:1060	arg1	WCA					1056:1058	WCA	1056:1058	WCA = 127°	1056:1065	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	2	41	theme	polycationic	401:412	arg1	polymers					414:421	three different natural polycationic polymers	377:421	three different natural polycationic polymers	377:421	We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth.					
33915214	2	42	theme	mammalian	488:496	arg1	growth					503:508	mammalian cell growth	488:508	mammalian cell growth	488:508	We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth.					
33915214	6	43	theme	TN/CAR	1017:1022	arg1	coating					1024:1030	The TN/CAR coating	1013:1030	The TN/CAR coating	1013:1030	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	5	44	theme	water	973:977	arg1	WCA					994:996	WCA	994:996	WCA	994:996	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	44	theme	water	973:977	arg1	angle					987:991	water contact angle	973:991	water contact angle (WCA) measurements	973:1010	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	4	45	theme	GG	758:759	arg1	film					761:764	the GG film	754:764	the GG film	754:764	PEMs (15-layers) are alternately deposited on the GG film.					
33915214	2	46	theme	natural	393:399	arg1	polymers					414:421	three different natural polycationic polymers	377:421	three different natural polycationic polymers	377:421	We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth.					
33915214	5	47	theme	coated	783:788	arg1	films					793:797	The GG film and coated GG films	767:797	films	793:797	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	0	48	theme	gum	125:127	arg1	films					129:133	gellan gum films	118:133	gellan gum films	118:133	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	3	49	theme	tannin	606:611	arg1	TN					625:626	TN	625:626	TN	625:626	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	3	49	theme	tannin	606:611	arg1	derivative					613:622	an amino-functionalized tannin derivative	582:622	an amino-functionalized tannin derivative (TN)	582:627	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	0	50	theme	chitosan	33:40	arg1	multilayers					103:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers	0:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.	0:134	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	2	51	theme	different	383:391	arg1	polymers					414:421	three different natural polycationic polymers	377:421	three different natural polycationic polymers	377:421	We show that PEMs composed of iota-carrageenan (CAR) and three different natural polycationic polymers have composition-dependent antimicrobial properties, and support mammalian cell growth.					
33915214	0	52	theme	N	45:45	arg1	multilayers					103:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers	0:113	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.	0:134	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	1	53	theme	layer-by-layer	295:308	arg1	approach					310:317	the layer-by-layer approach	291:317	the layer-by-layer approach	291:317	In this work free-standing gels formed from gellan gum (GG) by solvent evaporation are coated with polysaccharide-based polyelectrolyte multilayers, using the layer-by-layer approach.					
33915214	5	54	with	films	793:797	arg1	PEMs					804:807	PEMs	804:807	PEMs	804:807	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	55	theme	angle	987:991	arg1	measurements					999:1010	water contact angle (WCA) measurements	973:1010	water contact angle (WCA) measurements	973:1010	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	56	theme	force	945:949	arg1	AFM					963:965	AFM	963:965	AFM	963:965	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	56	theme	force	945:949	arg1	microscopy					951:960	atomic force microscopy	938:960	atomic force microscopy (AFM)	938:966	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	57	theme	infrared	830:837	arg1	spectroscopy					839:850	infrared spectroscopy	830:850	infrared spectroscopy with attenuated total reflectance (FTIR-ATR)	830:895	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	57	theme	infrared	830:837	arg1	FTIR-ATR					887:894	FTIR-ATR	887:894	FTIR-ATR	887:894	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	6	58	theme	243	1092:1094	arg1	surface					1078:1084	a hydrophobic (WCA = 127°) and rough surface	1041:1084	a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm)	1041:1103	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	58	theme	243	1092:1094	arg1	nm					1101:1102	Rq = 243 ± 48 nm	1087:1102	Rq = 243 ± 48 nm	1087:1102	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	5	59	with	film	774:777	arg1	PEMs					804:807	PEMs	804:807	PEMs	804:807	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	0	60	from	multilayers	103:113	arg1	films					129:133	gellan gum films	118:133	gellan gum films	118:133	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	6	61	theme	Rq	1087:1088	arg1	surface					1078:1084	a hydrophobic (WCA = 127°) and rough surface	1041:1084	a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm)	1041:1103	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	61	theme	Rq	1087:1088	arg1	nm					1101:1102	Rq = 243 ± 48 nm	1087:1102	Rq = 243 ± 48 nm	1087:1102	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	3	62	theme	Cationic	511:518	arg1	polymers					520:527	Cationic polymers	511:527	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN))	511:628	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	7	63	theme	antimicrobial	1298:1310	arg1	properties					1312:1321	antimicrobial properties	1298:1321	antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria	1298:1419	Polymer coatings promote stability and durability of the GG film, and introduce antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria.					
33915214	5	64	theme	atomic	938:943	arg1	AFM					963:965	AFM	963:965	AFM	963:965	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	64	theme	atomic	938:943	arg1	microscopy					951:960	atomic force microscopy	938:960	atomic force microscopy (AFM)	938:966	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	65	with	measurements	999:1010	arg1	reflectance					874:884	attenuated total reflectance	857:884	attenuated total reflectance	857:884	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	66	theme	photoelectron	904:916	arg1	XPS					932:934	XPS	932:934	XPS	932:934	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	5	66	theme	photoelectron	904:916	arg1	spectroscopy					918:929	X-ray photoelectron spectroscopy	898:929	X-ray photoelectron spectroscopy (XPS)	898:935	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	3	67	theme	amino-functionalized	585:604	arg1	TN					625:626	TN	625:626	TN	625:626	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	3	67	theme	amino-functionalized	585:604	arg1	derivative					613:622	an amino-functionalized tannin derivative	582:622	an amino-functionalized tannin derivative (TN)	582:627	Cationic polymers (chitosan (CHT), N,N,N-trimethyl chitosan (TMC), and an amino-functionalized tannin derivative (TN)) are individually assembled with the anionic iota-carrageenan (CAR) at pH 5.0.					
33915214	5	68	theme	attenuated	857:866	arg1	reflectance					874:884	attenuated total reflectance	857:884	attenuated total reflectance	857:884	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
33915214	7	69	theme	Gram-positive	1374:1386	arg1	bacteria					1412:1419	Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria	1331:1419	bacteria	1412:1419	Polymer coatings promote stability and durability of the GG film, and introduce antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria.					
33915214	0	70	theme	N-trimethyl	47:57	arg1	chitosan					59:66	N-trimethyl chitosan	47:66	N-trimethyl chitosan	47:66	Antimicrobial and cytocompatible chitosan, N,N,N-trimethyl chitosan, and tanfloc-based polyelectrolyte multilayers on gellan gum films.					
33915214	9	71	contain	have	1473:1476	arg2	packaging					1547:1555	food packaging	1542:1555	food packaging	1542:1555	Therefore, they have properties that can be further developed as wound dressings and food packaging.					
33915214	9	71	contain	have	1473:1476	arg2	properties					1478:1487	properties	1478:1487	properties that can be further developed as wound dressings and food packaging	1478:1555	Therefore, they have properties that can be further developed as wound dressings and food packaging.					
33915214	9	71	contain	have	1473:1476	arg1	they					1468:1471	they	1468:1471	they	1468:1471	Therefore, they have properties that can be further developed as wound dressings and food packaging.					
33915214	9	71	contain	have	1473:1476	arg2	dressings					1528:1536	wound dressings	1522:1536	wound dressings	1522:1536	Therefore, they have properties that can be further developed as wound dressings and food packaging.					
33915214	6	72	theme	rough	1072:1076	arg1	surface					1078:1084	a hydrophobic (WCA = 127°) and rough surface	1041:1084	a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm)	1041:1103	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	72	theme	rough	1072:1076	arg1	nm					1101:1102	Rq = 243 ± 48 nm	1087:1102	Rq = 243 ± 48 nm	1087:1102	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	73	dep	=	1060:1060	arg1	127°					1062:1065	127°	1062:1065	127°	1062:1065	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	7	74	dep	Gram-negative	1331:1343	arg1	enteritidis					1357:1367	Salmonella enteritidis	1346:1367	Salmonella enteritidis	1346:1367	Polymer coatings promote stability and durability of the GG film, and introduce antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria.					
33915214	1	75	theme	solvent	199:205	arg1	evaporation					207:217	solvent evaporation	199:217	solvent evaporation	199:217	In this work free-standing gels formed from gellan gum (GG) by solvent evaporation are coated with polysaccharide-based polyelectrolyte multilayers, using the layer-by-layer approach.					
33915214	6	76	theme	Rq	1200:1201	arg1	nm					1213:1214	Rq = 97 ± 12 nm	1200:1214	Rq = 97 ± 12 nm	1200:1214	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	6	76	theme	Rq	1200:1201	arg1	roughness					1189:1197	the lowest roughness	1178:1197	the lowest roughness (Rq = 97 ± 12 nm)	1178:1215	The TN/CAR coating provides a hydrophobic (WCA = 127°) and rough surface (Rq = 243 ± 48 nm), and the TMC/CAR coating provides a hydrophilic surface (WCA = 78°) with the lowest roughness (Rq = 97 ± 12 nm).					
33915214	7	77	theme	GG	1275:1276	arg1	film					1278:1281	the GG film	1271:1281	the GG film	1271:1281	Polymer coatings promote stability and durability of the GG film, and introduce antimicrobial properties against Gram-negative (Salmonella enteritidis) and Gram-positive (Staphylococcus aureus) bacteria.					
33915214	5	78	with	microscopy	951:960	arg1	reflectance					874:884	attenuated total reflectance	857:884	attenuated total reflectance	857:884	The GG film and coated GG films with PEMs are characterized by infrared spectroscopy with attenuated total reflectance (FTIR-ATR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and water contact angle (WCA) measurements.					
34540145	4	0	theme	gamma-glutamyltransferase	887:911	arg1	Redistribution					836:849	Redistribution	836:849	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans	836:941	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	5	1	dep	RESULTS	1066:1072	arg1	data					1086:1089	Comparative data	1074:1089	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count	1066:1162	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	3	2	theme	METHODS	696:702	arg1	Prostasomes					704:714	METHODS Prostasomes	696:714	METHODS Prostasomes	696:714	METHODS Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that maintained complete solubilization.					
34540145	8	3	dep	CONCLUSIONS	1628:1638	arg1	signature					1655:1663	Solubilization signature	1640:1663	CONCLUSIONS Solubilization signature	1628:1663	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	1	4	theme	distinct	266:273	arg1	TS					289:290	TS	289:290	TS	289:290	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	4	theme	distinct	266:273	arg1	tetraspanins					275:286	distinct tetraspanins	266:286	distinct tetraspanins (TS)	266:291	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	7	5	theme	molecular	1470:1478	arg1	patterns					1480:1487	two molecular patterns	1466:1487	two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins	1466:1625	Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins.					
34540145	2	6	theme	suitable	611:618	arg1	parameter					601:609	a new parameter	595:609	a new parameter suitable as a reference for the comparison of EVs populations in health and disease	595:693	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	7	7	gly	glycoproteins	1613:1625	arg1	glycoproteins					1613:1625	CD63/GGT/concanavalin A-reactive glycoproteins	1580:1625	CD63/GGT/concanavalin A-reactive glycoproteins	1580:1625	Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins.					
34540145	8	8	theme	distinction	1703:1713	arg1	factor					1715:1720	an all-inclusive distinction factor	1686:1720	an all-inclusive distinction factor regarding the surface properties of a particular vesicle	1686:1777	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	8	8	theme	distinction	1703:1713	arg1	CONCLUSIONS					1628:1638	CONCLUSIONS Solubilization signature	1628:1663	CONCLUSIONS Solubilization signature	1628:1663	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	1	9	theme	extracellular	191:203	arg1	vesicles					205:212	extracellular vesicles	191:212	extracellular vesicles (EVs) abundantly present in seminal plasma	191:255	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	9	theme	extracellular	191:203	arg1	EVs					215:217	EVs	215:217	EVs	215:217	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	9	theme	extracellular	191:203	arg1	Prostasomes					178:188	BACKGROUND Prostasomes	167:188	BACKGROUND Prostasomes	167:188	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	6	10	dep	this	1311:1314	arg1	contrast					1299:1306	contrast	1299:1306	contrast	1299:1306	In contrast to this, a greater difference was found in the redistribution of integral membrane proteins, exemplified by TS and gal-3.					
34540145	3	11	theme	gel	763:765	arg1	filtration					767:776	gel filtration	763:776	gel filtration	763:776	METHODS Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that maintained complete solubilization.					
34540145	2	12	theme	multimolecular	569:582	arg1	marker					584:589	an intrinsic multimolecular marker	556:589	an intrinsic multimolecular marker	556:589	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	8	13	theme	cell	1822:1825	arg1	status					1801:1806	the status	1797:1806	the status of the parent cell	1797:1825	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	8	13	theme	cell	1822:1825	arg1	environment					1849:1859	the extracellular environment	1831:1859	the extracellular environment	1831:1859	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	4	14	theme	distinct	924:931	arg1	N-glycans					933:941	distinct N-glycans	924:941	distinct N-glycans	924:941	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	5	15	theme	GGT	1239:1241	arg1	redistribution					1198:1211	the redistribution	1194:1211	the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat	1194:1293	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	1	16	theme	present	231:237	arg1	vesicles					205:212	extracellular vesicles	191:212	extracellular vesicles (EVs) abundantly present in seminal plasma	191:255	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	16	theme	present	231:237	arg1	EVs					215:217	EVs	215:217	EVs	215:217	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	16	theme	present	231:237	arg1	Prostasomes					178:188	BACKGROUND Prostasomes	167:188	BACKGROUND Prostasomes	167:188	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	2	17	theme	new	597:599	arg1	parameter					601:609	a new parameter	595:609	a new parameter suitable as a reference for the comparison of EVs populations in health and disease	595:693	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	1	18	theme	molecular	387:395	arg1	complexes					397:405	different molecular complexes	377:405	different molecular complexes	377:405	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	4	19	theme	gal-3	880:884	arg1	Redistribution					836:849	Redistribution	836:849	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans	836:941	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	4	20	theme	lectin-binding	975:988	arg1	assays					990:995	solid-phase lectin-binding assays	963:995	solid-phase lectin-binding assays	963:995	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	0	21	from	signature	88:96	arg1	men					162:164	normozoospermic and oligozoospermic men	126:164	normozoospermic and oligozoospermic men	126:164	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	3	22	theme	complete	811:818	arg1	solubilization					820:833	complete solubilization	811:833	complete solubilization	811:833	METHODS Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that maintained complete solubilization.					
34540145	6	23	theme	proteins	1391:1398	arg1	redistribution					1355:1368	the redistribution	1351:1368	the redistribution of integral membrane proteins, exemplified by TS and gal-3	1351:1427	In contrast to this, a greater difference was found in the redistribution of integral membrane proteins, exemplified by TS and gal-3.					
34540145	6	24	theme	integral	1373:1380	arg1	proteins					1391:1398	integral membrane proteins	1373:1398	integral membrane proteins	1373:1398	In contrast to this, a greater difference was found in the redistribution of integral membrane proteins, exemplified by TS and gal-3.					
34540145	5	25	theme	sperm	1152:1156	arg1	count					1158:1162	low sperm count	1148:1162	low sperm count	1148:1162	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	2	26	theme	populations	661:671	arg1	comparison					643:652	the comparison	639:652	the comparison of EVs populations in health and disease	639:693	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	5	27	theme	vesicle	1282:1288	arg1	coat					1290:1293	the vesicle coat	1278:1293	the vesicle coat	1278:1293	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	5	28	theme	normal	1112:1117	arg1	physiology					1119:1128	normal physiology	1112:1128	normal physiology	1112:1128	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	7	29	theme	A-reactive	1602:1611	arg1	glycoproteins					1613:1625	CD63/GGT/concanavalin A-reactive glycoproteins	1580:1625	CD63/GGT/concanavalin A-reactive glycoproteins	1580:1625	Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins.					
34540145	0	30	theme	seminal	101:107	arg1	prostasomes					109:119	seminal prostasomes	101:119	seminal prostasomes from normozoospermic and oligozoospermic men	101:164	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	2	31	theme	prostasomal	477:487	arg1	proteins					489:496	membrane-associated prostasomal proteins	457:496	membrane-associated prostasomal proteins	457:496	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	8	32	theme	spatial	1909:1915	arg1	arrangements					1926:1937	spatial membrane arrangements	1909:1937	spatial membrane arrangements	1909:1937	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	2	33	theme	detergent-sensitivity	423:443	arg1	patterns					445:452	detergent-sensitivity patterns	423:452	detergent-sensitivity patterns of membrane-associated prostasomal proteins	423:496	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	8	34	theme	arrangements	1926:1937	arg1	composition					1894:1904	the composition	1890:1904	the composition of spatial membrane arrangements	1890:1937	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	4	35	theme	lectin	1053:1058	arg1	blot					1060:1063	lectin blot	1053:1063	lectin blot	1053:1063	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	0	36	theme	tetraspanins	12:23	arg1	Assembly					0:7	Assembly	0:7	Assembly of tetraspanins, galectin-3, and distinct N-glycans	0:59	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	5	37	theme	distinct	1216:1223	arg1	all					1244:1246	all	1244:1246	all	1244:1246	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	5	37	theme	distinct	1216:1223	arg1	N-glycans					1225:1233	distinct N-glycans	1216:1233	distinct N-glycans	1216:1233	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	4	38	theme	electron	1011:1018	arg1	microscopy					1020:1029	transmission electron microscopy	998:1029	transmission electron microscopy	998:1029	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	4	39	theme	TS	854:855	arg1	Redistribution					836:849	Redistribution	836:849	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans	836:941	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	0	40	theme	galectin-3	26:35	arg1	Assembly					0:7	Assembly	0:7	Assembly of tetraspanins, galectin-3, and distinct N-glycans	0:59	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	2	41	theme	membrane-associated	457:475	arg1	proteins					489:496	membrane-associated prostasomal proteins	457:496	membrane-associated prostasomal proteins	457:496	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	7	42	theme	germ	1537:1540	arg1	glycoproteins					1562:1574	overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins	1510:1574	overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins	1510:1574	Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins.					
34540145	0	43	theme	N-glycans	51:59	arg1	Assembly					0:7	Assembly	0:7	Assembly of tetraspanins, galectin-3, and distinct N-glycans	0:59	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	7	44	theme	overlapped	1510:1519	arg1	glycoproteins					1562:1574	overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins	1510:1574	overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins	1510:1574	Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins.					
34540145	6	45	theme	membrane	1382:1389	arg1	proteins					1391:1398	integral membrane proteins	1373:1398	integral membrane proteins	1373:1398	In contrast to this, a greater difference was found in the redistribution of integral membrane proteins, exemplified by TS and gal-3.					
34540145	8	46	theme	all-inclusive	1689:1701	arg1	factor					1715:1720	an all-inclusive distinction factor	1686:1720	an all-inclusive distinction factor regarding the surface properties of a particular vesicle	1686:1777	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	8	46	theme	all-inclusive	1689:1701	arg1	CONCLUSIONS					1628:1638	CONCLUSIONS Solubilization signature	1628:1663	CONCLUSIONS Solubilization signature	1628:1663	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	8	47	theme	vesicle	1771:1777	arg1	properties					1744:1753	the surface properties	1732:1753	the surface properties of a particular vesicle	1732:1777	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	0	48	from	men	162:164	arg1	signature					88:96	the solubilization signature	69:96	the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men	69:164	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	0	48	from	men	162:164	arg1	prostasomes					109:119	seminal prostasomes	101:119	seminal prostasomes from normozoospermic and oligozoospermic men	101:164	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	6	49	located	found	1342:1346	arg1	redistribution					1355:1368	the redistribution	1351:1368	the redistribution of integral membrane proteins, exemplified by TS and gal-3	1351:1427	In contrast to this, a greater difference was found in the redistribution of integral membrane proteins, exemplified by TS and gal-3.					
34540145	6	49	located	found	1342:1346	arg2	difference					1327:1336	a greater difference	1317:1336	a greater difference	1317:1336	In contrast to this, a greater difference was found in the redistribution of integral membrane proteins, exemplified by TS and gal-3.					
34540145	3	50	theme	Triton	734:739	arg1	X-100					741:745	Triton X-100	734:745	Triton X-100	734:745	METHODS Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that maintained complete solubilization.					
34540145	0	51	theme	normozoospermic	126:140	arg1	men					162:164	normozoospermic and oligozoospermic men	126:164	normozoospermic and oligozoospermic men	126:164	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	2	52	theme	intrinsic	559:567	arg1	marker					584:589	an intrinsic multimolecular marker	556:589	an intrinsic multimolecular marker	556:589	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	0	53	theme	oligozoospermic	146:160	arg1	men					162:164	normozoospermic and oligozoospermic men	126:164	normozoospermic and oligozoospermic men	126:164	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	8	54	theme	parent	1815:1820	arg1	cell					1822:1825	the parent cell	1811:1825	the parent cell	1811:1825	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	4	55	dep	TS	854:855	arg1	CD9					864:866	CD9	864:866	CD9	864:866	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	4	55	dep	TS	854:855	arg1	CD81					873:876	CD81	873:876	CD81	873:876	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	1	56	from	plasma	250:255	arg1	present					231:237	present	231:237	present	231:237	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	57	theme	different	377:385	arg1	complexes					397:405	different molecular complexes	377:405	different molecular complexes	377:405	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	58	from	present	231:237	arg1	plasma					250:255	seminal plasma	242:255	seminal plasma	242:255	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	5	59	theme	coat	1290:1293	arg1	part					1270:1273	part	1270:1273	part of the vesicle coat	1270:1293	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	1	60	theme	complexes	397:405	arg1	assembly					365:372	an assembly	362:372	an assembly of different molecular complexes	362:405	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	8	61	theme	extracellular	1835:1847	arg1	environment					1849:1859	the extracellular environment	1831:1859	the extracellular environment	1831:1859	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	2	62	from	populations	661:671	arg1	disease					687:693	disease	687:693	disease	687:693	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	2	62	from	populations	661:671	arg1	health					676:681	health	676:681	health	676:681	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	5	63	theme	Comparative	1074:1084	arg1	data					1086:1089	Comparative data	1074:1089	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count	1066:1162	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	4	64	theme	solid-phase	963:973	arg1	assays					990:995	solid-phase lectin-binding assays	963:995	solid-phase lectin-binding assays	963:995	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	2	65	from	health	676:681	arg1	comparison					643:652	the comparison	639:652	the comparison of EVs populations in health and disease	639:693	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	4	66	theme	N-glycans	933:941	arg1	Redistribution					836:849	Redistribution	836:849	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans	836:941	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	0	67	theme	solubilization	73:86	arg1	signature					88:96	the solubilization signature	69:96	the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men	69:164	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	5	68	theme	low	1148:1150	arg1	count					1158:1162	low sperm count	1148:1162	low sperm count	1148:1162	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	1	69	theme	BACKGROUND	167:176	arg1	vesicles					205:212	extracellular vesicles	191:212	extracellular vesicles (EVs) abundantly present in seminal plasma	191:255	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	69	theme	BACKGROUND	167:176	arg1	Prostasomes					178:188	BACKGROUND Prostasomes	167:188	BACKGROUND Prostasomes	167:188	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	7	70	gly	glycoproteins	1562:1574	arg1	glycoproteins					1562:1574	overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins	1510:1574	overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins	1510:1574	Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins.					
34540145	2	71	theme	proteins	489:496	arg1	patterns					445:452	detergent-sensitivity patterns	423:452	detergent-sensitivity patterns of membrane-associated prostasomal proteins	423:496	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	2	72	from	disease	687:693	arg1	comparison					643:652	the comparison	639:652	the comparison of EVs populations in health and disease	639:693	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	5	73	theme	count	1158:1162	arg1	conditions					1134:1143	conditions	1134:1143	conditions of low sperm count	1134:1162	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	5	73	theme	count	1158:1162	arg1	prostasomes					1094:1104	prostasomes	1094:1104	prostasomes under normal physiology	1094:1128	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	7	74	theme	CD63/GGT/concanavalin	1580:1600	arg1	glycoproteins					1613:1625	CD63/GGT/concanavalin A-reactive glycoproteins	1580:1625	CD63/GGT/concanavalin A-reactive glycoproteins	1580:1625	Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins.					
34540145	2	75	theme	solubilization	528:541	arg1	signature					543:551	the solubilization signature	524:551	the solubilization signature	524:551	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	2	76	theme	EVs	657:659	arg1	populations					661:671	EVs populations	657:671	EVs populations in health and disease	657:693	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	5	77	from	data	1086:1089	arg1	conditions					1134:1143	conditions	1134:1143	conditions of low sperm count	1134:1162	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	5	77	from	data	1086:1089	arg1	prostasomes					1094:1104	prostasomes	1094:1104	prostasomes under normal physiology	1094:1128	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	0	78	theme	prostasomes	109:119	arg1	signature					88:96	the solubilization signature	69:96	the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men	69:164	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	8	79	theme	membrane	1917:1924	arg1	arrangements					1926:1937	spatial membrane arrangements	1909:1937	spatial membrane arrangements	1909:1937	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	5	80	theme	N-glycans	1225:1233	arg1	redistribution					1198:1211	the redistribution	1194:1211	the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat	1194:1293	RESULTS Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat.					
34540145	4	81	theme	transmission	998:1009	arg1	microscopy					1020:1029	transmission electron microscopy	998:1029	transmission electron microscopy	998:1029	Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot.					
34540145	8	82	theme	Solubilization	1640:1653	arg1	signature					1655:1663	Solubilization signature	1640:1663	CONCLUSIONS Solubilization signature	1628:1663	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	0	83	theme	distinct	42:49	arg1	N-glycans					51:59	distinct N-glycans	42:59	distinct N-glycans	42:59	Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men.					
34540145	7	84	theme	agglutinin-reactive	1542:1560	arg1	glycoproteins					1562:1574	overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins	1510:1574	overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins	1510:1574	Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins.					
34540145	6	85	theme	greater	1319:1325	arg1	difference					1327:1336	a greater difference	1317:1336	a greater difference	1317:1336	In contrast to this, a greater difference was found in the redistribution of integral membrane proteins, exemplified by TS and gal-3.					
34540145	2	86	from	comparison	643:652	arg1	disease					687:693	disease	687:693	disease	687:693	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	2	86	from	comparison	643:652	arg1	health					676:681	health	676:681	health	676:681	In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease.					
34540145	8	87	theme	surface	1736:1742	arg1	properties					1744:1753	the surface properties	1732:1753	the surface properties of a particular vesicle	1732:1777	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34540145	7	88	theme	CD9/gal-3/wheat	1521:1535	arg1	glycoproteins					1562:1574	overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins	1510:1574	overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins	1510:1574	Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/GGT/concanavalin A-reactive glycoproteins.					
34540145	1	89	attach	present	231:237	arg1	plasma					250:255	seminal plasma	242:255	seminal plasma	242:255	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	89	attach	present	231:237	arg2	Prostasomes					178:188	BACKGROUND Prostasomes	167:188	BACKGROUND Prostasomes	167:188	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	89	attach	present	231:237	arg2	EVs					215:217	EVs	215:217	EVs	215:217	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	89	attach	present	231:237	arg2	vesicles					205:212	extracellular vesicles	191:212	extracellular vesicles (EVs) abundantly present in seminal plasma	191:255	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	1	90	theme	seminal	242:248	arg1	plasma					250:255	seminal plasma	242:255	seminal plasma	242:255	BACKGROUND Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes.					
34540145	8	91	theme	particular	1760:1769	arg1	vesicle					1771:1777	a particular vesicle	1758:1777	a particular vesicle	1758:1777	CONCLUSIONS Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.					
34469105	5	0	theme	saccharide	767:776	arg1	fragments					778:786	new saccharide fragments	763:786	new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens	763:914	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	9	1	theme	new	1265:1267	arg1	compounds					1269:1277	The six new compounds	1257:1277	The six new compounds	1257:1277	The six new compounds were tested with a glycan array to evaluate their recognition by antibodies in reference group 19 antisera and factor reference antisera (reacting against 19F or 19A).					
34469105	9	2	theme	reference	1358:1366	arg1	group					1368:1372	reference group	1358:1372	reference group 19 antisera	1358:1384	The six new compounds were tested with a glycan array to evaluate their recognition by antibodies in reference group 19 antisera and factor reference antisera (reacting against 19F or 19A).					
34469105	4	3	theme	formulation	598:608	arg1	development					563:573	the development	559:573	the development of efficacious vaccine formulation	559:608	Indeed, the development of efficacious vaccine formulation is complicated by the high number of bacterial serotypes with different CPSs.					
34469105	5	4	theme	fragments	778:786	arg1	design					753:758	the design	749:758	the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens	749:914	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	6	5	theme	Streptococcus	946:958	arg1	Sp					972:973	Sp	972:973	Sp	972:973	In particular, we focused on Streptococcus pneumoniae (Sp) 19A and 19F.					
34469105	6	5	theme	Streptococcus	946:958	arg1	pneumoniae					960:969	Streptococcus pneumoniae	946:969	Streptococcus pneumoniae (Sp) 19A	946:978	In particular, we focused on Streptococcus pneumoniae (Sp) 19A and 19F.					
34469105	9	6	from	antibodies	1344:1353	arg1	group					1368:1372	reference group	1358:1372	reference group 19 antisera	1358:1384	The six new compounds were tested with a glycan array to evaluate their recognition by antibodies in reference group 19 antisera and factor reference antisera (reacting against 19F or 19A).					
34469105	9	6	from	antibodies	1344:1353	arg1	antisera					1407:1414	factor reference antisera	1390:1414	factor reference antisera (reacting against 19F or 19A)	1390:1444	The six new compounds were tested with a glycan array to evaluate their recognition by antibodies in reference group 19 antisera and factor reference antisera (reacting against 19F or 19A).					
34469105	10	7	theme	factor	1636:1641	arg1	antisera					1643:1650	factor antisera	1636:1650	factor antisera	1636:1650	The disaccharide A-B, phosphorylated at the upstream end, emerged as a hit from the glycan array screening because it is strongly recognized by the group 19 antisera and by the 19F and 19A factor antisera, with similar intensity compared with the CPSs used as controls.					
34469105	10	7	theme	factor	1636:1641	arg1	hit					1518:1520	a hit	1516:1520	a hit from the glycan array screening	1516:1552	The disaccharide A-B, phosphorylated at the upstream end, emerged as a hit from the glycan array screening because it is strongly recognized by the group 19 antisera and by the 19F and 19A factor antisera, with similar intensity compared with the CPSs used as controls.					
34469105	4	8	theme	vaccine	590:596	arg1	formulation					598:608	efficacious vaccine formulation	578:608	efficacious vaccine formulation	578:608	Indeed, the development of efficacious vaccine formulation is complicated by the high number of bacterial serotypes with different CPSs.					
34469105	7	9	theme	1→4	1105:1107	arg1	structure					1067:1075	a common structure	1058:1075	a common structure	1058:1075	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	7	9	theme	1→4	1105:1107	arg1	A-B					1115:1117	A-B	1115:1117	A-B	1115:1117	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	7	9	theme	1→4	1105:1107	arg1	-Glc					1109:1112	the disaccharide ManNAc-β-(1→4)-Glc	1078:1112	the disaccharide ManNAc-β-(1→4)-Glc (A-B)	1078:1118	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	9	10	theme	factor	1390:1395	arg1	antisera					1407:1414	factor reference antisera	1390:1414	factor reference antisera (reacting against 19F or 19A)	1390:1444	The six new compounds were tested with a glycan array to evaluate their recognition by antibodies in reference group 19 antisera and factor reference antisera (reacting against 19F or 19A).					
34469105	3	11	theme	specific	378:385	arg1	serotypes					395:403	the specific vaccine serotypes	374:403	the specific vaccine serotypes	374:403	Protection is largely limited to the specific vaccine serotypes, and the continuous need for broader coverage to control the outbreak of emerging serotypes is pushing the development of new vaccine candidates.					
34469105	8	12	theme	19F/19A	1235:1241	arg1	disaccharide					1243:1254	the common 19F/19A disaccharide	1224:1254	the common 19F/19A disaccharide	1224:1254	Herein, we describe the synthesis of a small library of compounds containing different combinations of the common 19F/19A disaccharide.					
34469105	11	13	theme	phosphorylated	1760:1773	arg1	A-B					1788:1790	the phosphorylated disaccharide A-B	1756:1790	the phosphorylated disaccharide A-B	1756:1790	Our data give a strong indication that the phosphorylated disaccharide A-B can be considered a common epitope among different Sp 19 serotypes.					
34469105	7	14	theme	ManNAc-β-	1095:1103	arg1	structure					1067:1075	a common structure	1058:1075	a common structure	1058:1075	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	7	14	theme	ManNAc-β-	1095:1103	arg1	A-B					1115:1117	A-B	1115:1117	A-B	1115:1117	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	7	14	theme	ManNAc-β-	1095:1103	arg1	-Glc					1109:1112	the disaccharide ManNAc-β-(1→4)-Glc	1078:1112	the disaccharide ManNAc-β-(1→4)-Glc (A-B)	1078:1118	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	8	15	theme	common	1228:1233	arg1	disaccharide					1243:1254	the common 19F/19A disaccharide	1224:1254	the common 19F/19A disaccharide	1224:1254	Herein, we describe the synthesis of a small library of compounds containing different combinations of the common 19F/19A disaccharide.					
34469105	3	16	theme	candidates	539:548	arg1	development					512:522	the development	508:522	the development of new vaccine candidates	508:548	Protection is largely limited to the specific vaccine serotypes, and the continuous need for broader coverage to control the outbreak of emerging serotypes is pushing the development of new vaccine candidates.					
34469105	1	17	theme	pneumococcal	192:203	arg1	diseases					205:212	invasive pneumococcal diseases	183:212	invasive pneumococcal diseases	183:212	Vaccination represents the most effective way to prevent invasive pneumococcal diseases.					
34469105	5	18	theme	common	900:905	arg1	antigens					907:914	cross-reactive and potentially cross-protective common antigens	852:914	cross-reactive and potentially cross-protective common antigens	852:914	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	8	19	theme	compounds	1177:1185	arg1	library					1166:1172	a small library	1158:1172	a small library of compounds containing different combinations of the common 19F/19A disaccharide	1158:1254	Herein, we describe the synthesis of a small library of compounds containing different combinations of the common 19F/19A disaccharide.					
34469105	11	20	theme	Sp	1843:1844	arg1	serotypes					1849:1857	different Sp 19 serotypes	1833:1857	different Sp 19 serotypes	1833:1857	Our data give a strong indication that the phosphorylated disaccharide A-B can be considered a common epitope among different Sp 19 serotypes.					
34469105	5	21	theme	different	829:837	arg1	serotypes					839:847	different serotypes	829:847	different serotypes as cross-reactive and potentially cross-protective common antigens	829:914	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	1	22	theme	invasive	183:190	arg1	diseases					205:212	invasive pneumococcal diseases	183:212	invasive pneumococcal diseases	183:212	Vaccination represents the most effective way to prevent invasive pneumococcal diseases.					
34469105	0	23	theme	Array	7:11	arg1	Evaluation					13:22	Glycan Array Evaluation	0:22	Glycan Array Evaluation of Synthetic Epitopes between the Capsular Polysaccharides from Streptococcus pneumoniae	0:111	Glycan Array Evaluation of Synthetic Epitopes between the Capsular Polysaccharides from Streptococcus pneumoniae 19F and 19A.					
34469105	10	24	theme	upstream	1491:1498	arg1	end					1500:1502	the upstream end	1487:1502	the upstream end	1487:1502	The disaccharide A-B, phosphorylated at the upstream end, emerged as a hit from the glycan array screening because it is strongly recognized by the group 19 antisera and by the 19F and 19A factor antisera, with similar intensity compared with the CPSs used as controls.					
34469105	10	25	theme	array	1538:1542	arg1	screening					1544:1552	the glycan array screening	1527:1552	the glycan array screening	1527:1552	The disaccharide A-B, phosphorylated at the upstream end, emerged as a hit from the glycan array screening because it is strongly recognized by the group 19 antisera and by the 19F and 19A factor antisera, with similar intensity compared with the CPSs used as controls.					
34469105	2	26	theme	serotypes	330:338	arg1	CPSs					303:306	CPSs	303:306	CPSs	303:306	The glycoconjugate vaccines licensed so far are obtained from capsular polysaccharides (CPSs) of the most virulent serotypes.					
34469105	2	26	theme	serotypes	330:338	arg1	polysaccharides					286:300	capsular polysaccharides	277:300	capsular polysaccharides (CPSs) of the most virulent serotypes	277:338	The glycoconjugate vaccines licensed so far are obtained from capsular polysaccharides (CPSs) of the most virulent serotypes.					
34469105	10	27	theme	glycan	1531:1536	arg1	screening					1544:1552	the glycan array screening	1527:1552	the glycan array screening	1527:1552	The disaccharide A-B, phosphorylated at the upstream end, emerged as a hit from the glycan array screening because it is strongly recognized by the group 19 antisera and by the 19F and 19A factor antisera, with similar intensity compared with the CPSs used as controls.					
34469105	3	28	theme	broader	434:440	arg1	coverage					442:449	broader coverage	434:449	broader coverage	434:449	Protection is largely limited to the specific vaccine serotypes, and the continuous need for broader coverage to control the outbreak of emerging serotypes is pushing the development of new vaccine candidates.					
34469105	0	29	theme	Glycan	0:5	arg1	Evaluation					13:22	Glycan Array Evaluation	0:22	Glycan Array Evaluation of Synthetic Epitopes between the Capsular Polysaccharides from Streptococcus pneumoniae	0:111	Glycan Array Evaluation of Synthetic Epitopes between the Capsular Polysaccharides from Streptococcus pneumoniae 19F and 19A.					
34469105	9	30	theme	glycan	1298:1303	arg1	array					1305:1309	a glycan array	1296:1309	a glycan array to evaluate their recognition by antibodies in reference group 19 antisera and factor reference antisera (reacting against 19F or 19A)	1296:1444	The six new compounds were tested with a glycan array to evaluate their recognition by antibodies in reference group 19 antisera and factor reference antisera (reacting against 19F or 19A).					
34469105	5	31	contain	containing	788:797	arg2	structures					808:817	chemical structures	799:817	chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens	799:914	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	5	31	contain	containing	788:797	arg1	fragments					778:786	new saccharide fragments	763:786	new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens	763:914	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	2	32	theme	virulent	321:328	arg1	serotypes					330:338	the most virulent serotypes	312:338	the most virulent serotypes	312:338	The glycoconjugate vaccines licensed so far are obtained from capsular polysaccharides (CPSs) of the most virulent serotypes.					
34469105	11	33	theme	common	1812:1817	arg1	epitope					1819:1825	a common epitope	1810:1825	a common epitope among different Sp 19 serotypes	1810:1857	Our data give a strong indication that the phosphorylated disaccharide A-B can be considered a common epitope among different Sp 19 serotypes.					
34469105	3	34	theme	vaccine	387:393	arg1	serotypes					395:403	the specific vaccine serotypes	374:403	the specific vaccine serotypes	374:403	Protection is largely limited to the specific vaccine serotypes, and the continuous need for broader coverage to control the outbreak of emerging serotypes is pushing the development of new vaccine candidates.					
34469105	8	35	theme	small	1160:1164	arg1	library					1166:1172	a small library	1158:1172	a small library of compounds containing different combinations of the common 19F/19A disaccharide	1158:1254	Herein, we describe the synthesis of a small library of compounds containing different combinations of the common 19F/19A disaccharide.					
34469105	4	36	theme	bacterial	647:655	arg1	serotypes					657:665	bacterial serotypes	647:665	bacterial serotypes with different CPSs	647:685	Indeed, the development of efficacious vaccine formulation is complicated by the high number of bacterial serotypes with different CPSs.					
34469105	7	37	theme	Sp	1016:1017	arg1	19F					1019:1021	Sp 19F	1016:1021	Sp 19F	1016:1021	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	4	38	theme	efficacious	578:588	arg1	formulation					598:608	efficacious vaccine formulation	578:608	efficacious vaccine formulation	578:608	Indeed, the development of efficacious vaccine formulation is complicated by the high number of bacterial serotypes with different CPSs.					
34469105	5	39	theme	vaccine	717:723	arg1	composition					725:735	vaccine composition	717:735	vaccine composition	717:735	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	9	40	dep	group	1368:1372	arg1	antisera					1377:1384	19 antisera	1374:1384	reference group 19 antisera	1358:1384	The six new compounds were tested with a glycan array to evaluate their recognition by antibodies in reference group 19 antisera and factor reference antisera (reacting against 19F or 19A).					
34469105	9	41	theme	reference	1397:1405	arg1	antisera					1407:1414	factor reference antisera	1390:1414	factor reference antisera (reacting against 19F or 19A)	1390:1444	The six new compounds were tested with a glycan array to evaluate their recognition by antibodies in reference group 19 antisera and factor reference antisera (reacting against 19F or 19A).					
34469105	3	42	theme	emerging	478:485	arg1	serotypes					487:495	emerging serotypes	478:495	emerging serotypes	478:495	Protection is largely limited to the specific vaccine serotypes, and the continuous need for broader coverage to control the outbreak of emerging serotypes is pushing the development of new vaccine candidates.					
34469105	0	43	theme	Epitopes	37:44	arg1	Evaluation					13:22	Glycan Array Evaluation	0:22	Glycan Array Evaluation of Synthetic Epitopes between the Capsular Polysaccharides from Streptococcus pneumoniae	0:111	Glycan Array Evaluation of Synthetic Epitopes between the Capsular Polysaccharides from Streptococcus pneumoniae 19F and 19A.					
34469105	7	44	dep	CPS	993:995	arg1	repeating					997:1005	repeating	997:1005	repeating units of Sp 19F	997:1021	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	7	44	dep	CPS	993:995	arg1	19A					1027:1029	19A	1027:1029	19A	1027:1029	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	11	45	theme	different	1833:1841	arg1	serotypes					1849:1857	different Sp 19 serotypes	1833:1857	different Sp 19 serotypes	1833:1857	Our data give a strong indication that the phosphorylated disaccharide A-B can be considered a common epitope among different Sp 19 serotypes.					
34469105	0	46	theme	Synthetic	27:35	arg1	Epitopes					37:44	Synthetic Epitopes	27:44	Synthetic Epitopes between the Capsular Polysaccharides from Streptococcus pneumoniae	27:111	Glycan Array Evaluation of Synthetic Epitopes between the Capsular Polysaccharides from Streptococcus pneumoniae 19F and 19A.					
34469105	11	47	theme	strong	1733:1738	arg1	indication					1740:1749	a strong indication	1731:1749	a strong indication that the phosphorylated disaccharide A-B can be considered a common epitope among different Sp 19 serotypes	1731:1857	Our data give a strong indication that the phosphorylated disaccharide A-B can be considered a common epitope among different Sp 19 serotypes.					
34469105	5	48	theme	chemical	799:806	arg1	structures					808:817	chemical structures	799:817	chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens	799:914	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	8	49	contain	containing	1187:1196	arg1	compounds					1177:1185	compounds	1177:1185	compounds containing different combinations of the common 19F/19A disaccharide	1177:1254	Herein, we describe the synthesis of a small library of compounds containing different combinations of the common 19F/19A disaccharide.					
34469105	8	49	contain	containing	1187:1196	arg2	combinations					1208:1219	different combinations	1198:1219	different combinations of the common 19F/19A disaccharide	1198:1254	Herein, we describe the synthesis of a small library of compounds containing different combinations of the common 19F/19A disaccharide.					
34469105	5	50	theme	cross-protective	883:898	arg1	antigens					907:914	cross-reactive and potentially cross-protective common antigens	852:914	cross-reactive and potentially cross-protective common antigens	852:914	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	4	51	theme	serotypes	657:665	arg1	number					637:642	the high number	628:642	the high number of bacterial serotypes with different CPSs	628:685	Indeed, the development of efficacious vaccine formulation is complicated by the high number of bacterial serotypes with different CPSs.					
34469105	11	52	dep	indication	1740:1749	arg1	considered					1799:1808	considered	1799:1808	can be considered a common epitope among different Sp 19 serotypes	1792:1857	Our data give a strong indication that the phosphorylated disaccharide A-B can be considered a common epitope among different Sp 19 serotypes.					
34469105	8	53	theme	library	1166:1172	arg1	synthesis					1145:1153	the synthesis	1141:1153	the synthesis of a small library of compounds containing different combinations of the common 19F/19A disaccharide	1141:1254	Herein, we describe the synthesis of a small library of compounds containing different combinations of the common 19F/19A disaccharide.					
34469105	5	54	theme	new	763:765	arg1	fragments					778:786	new saccharide fragments	763:786	new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens	763:914	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	9	55	dep	antisera	1407:1414	arg1	reacting					1417:1424	reacting	1417:1424	reacting against 19F or 19A	1417:1443	The six new compounds were tested with a glycan array to evaluate their recognition by antibodies in reference group 19 antisera and factor reference antisera (reacting against 19F or 19A).					
34469105	7	56	theme	common	1060:1065	arg1	-Glc					1109:1112	the disaccharide ManNAc-β-(1→4)-Glc	1078:1112	the disaccharide ManNAc-β-(1→4)-Glc (A-B)	1078:1118	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	7	56	theme	common	1060:1065	arg1	structure					1067:1075	a common structure	1058:1075	a common structure	1058:1075	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	3	57	theme	continuous	414:423	arg1	need					425:428	the continuous need	410:428	the continuous need for broader coverage to control the outbreak of emerging serotypes	410:495	Protection is largely limited to the specific vaccine serotypes, and the continuous need for broader coverage to control the outbreak of emerging serotypes is pushing the development of new vaccine candidates.					
34469105	4	58	with	serotypes	657:665	arg1	CPSs					682:685	different CPSs	672:685	different CPSs	672:685	Indeed, the development of efficacious vaccine formulation is complicated by the high number of bacterial serotypes with different CPSs.					
34469105	7	59	theme	19F	1019:1021	arg1	units					1007:1011	units	1007:1011	units of Sp 19F	1007:1021	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	11	60	theme	disaccharide	1775:1786	arg1	A-B					1788:1790	the phosphorylated disaccharide A-B	1756:1790	the phosphorylated disaccharide A-B	1756:1790	Our data give a strong indication that the phosphorylated disaccharide A-B can be considered a common epitope among different Sp 19 serotypes.					
34469105	2	61	theme	glycoconjugate	219:232	arg1	vaccines					234:241	The glycoconjugate vaccines	215:241	The glycoconjugate vaccines licensed so far	215:257	The glycoconjugate vaccines licensed so far are obtained from capsular polysaccharides (CPSs) of the most virulent serotypes.					
34469105	3	62	theme	serotypes	487:495	arg1	outbreak					466:473	the outbreak	462:473	the outbreak of emerging serotypes	462:495	Protection is largely limited to the specific vaccine serotypes, and the continuous need for broader coverage to control the outbreak of emerging serotypes is pushing the development of new vaccine candidates.					
34469105	8	63	theme	disaccharide	1243:1254	arg1	combinations					1208:1219	different combinations	1198:1219	different combinations of the common 19F/19A disaccharide	1198:1254	Herein, we describe the synthesis of a small library of compounds containing different combinations of the common 19F/19A disaccharide.					
34469105	10	64	theme	similar	1658:1664	arg1	intensity					1666:1674	similar intensity	1658:1674	similar intensity	1658:1674	The disaccharide A-B, phosphorylated at the upstream end, emerged as a hit from the glycan array screening because it is strongly recognized by the group 19 antisera and by the 19F and 19A factor antisera, with similar intensity compared with the CPSs used as controls.					
34469105	2	65	theme	capsular	277:284	arg1	CPSs					303:306	CPSs	303:306	CPSs	303:306	The glycoconjugate vaccines licensed so far are obtained from capsular polysaccharides (CPSs) of the most virulent serotypes.					
34469105	2	65	theme	capsular	277:284	arg1	polysaccharides					286:300	capsular polysaccharides	277:300	capsular polysaccharides (CPSs) of the most virulent serotypes	277:338	The glycoconjugate vaccines licensed so far are obtained from capsular polysaccharides (CPSs) of the most virulent serotypes.					
34469105	10	66	from	screening	1544:1552	arg1	antisera					1643:1650	factor antisera	1636:1650	factor antisera	1636:1650	The disaccharide A-B, phosphorylated at the upstream end, emerged as a hit from the glycan array screening because it is strongly recognized by the group 19 antisera and by the 19F and 19A factor antisera, with similar intensity compared with the CPSs used as controls.					
34469105	10	66	from	screening	1544:1552	arg1	hit					1518:1520	a hit	1516:1520	a hit from the glycan array screening	1516:1552	The disaccharide A-B, phosphorylated at the upstream end, emerged as a hit from the glycan array screening because it is strongly recognized by the group 19 antisera and by the 19F and 19A factor antisera, with similar intensity compared with the CPSs used as controls.					
34469105	1	67	theme	effective	158:166	arg1	way					168:170	the most effective way	149:170	the most effective way to prevent invasive pneumococcal diseases	149:212	Vaccination represents the most effective way to prevent invasive pneumococcal diseases.					
34469105	7	68	theme	disaccharide	1082:1093	arg1	structure					1067:1075	a common structure	1058:1075	a common structure	1058:1075	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	7	68	theme	disaccharide	1082:1093	arg1	A-B					1115:1117	A-B	1115:1117	A-B	1115:1117	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	7	68	theme	disaccharide	1082:1093	arg1	-Glc					1109:1112	the disaccharide ManNAc-β-(1→4)-Glc	1078:1112	the disaccharide ManNAc-β-(1→4)-Glc (A-B)	1078:1118	The CPS repeating units of Sp 19F and 19A are very similar and share a common structure, the disaccharide ManNAc-β-(1→4)-Glc (A-B).					
34469105	10	69	theme	disaccharide	1451:1462	arg1	A-B					1464:1466	The disaccharide A-B	1447:1466	The disaccharide A-B	1447:1466	The disaccharide A-B, phosphorylated at the upstream end, emerged as a hit from the glycan array screening because it is strongly recognized by the group 19 antisera and by the 19F and 19A factor antisera, with similar intensity compared with the CPSs used as controls.					
34469105	0	70	from	pneumoniae	102:111	arg1	Polysaccharides					67:81	the Capsular Polysaccharides	54:81	the Capsular Polysaccharides from Streptococcus pneumoniae	54:111	Glycan Array Evaluation of Synthetic Epitopes between the Capsular Polysaccharides from Streptococcus pneumoniae 19F and 19A.					
34469105	5	71	theme	cross-reactive	852:865	arg1	antigens					907:914	cross-reactive and potentially cross-protective common antigens	852:914	cross-reactive and potentially cross-protective common antigens	852:914	In this context, to simplify vaccine composition, we propose the design of new saccharide fragments containing chemical structures shared by different serotypes as cross-reactive and potentially cross-protective common antigens.					
34469105	3	72	theme	new	527:529	arg1	candidates					539:548	new vaccine candidates	527:548	new vaccine candidates	527:548	Protection is largely limited to the specific vaccine serotypes, and the continuous need for broader coverage to control the outbreak of emerging serotypes is pushing the development of new vaccine candidates.					
34469105	4	73	theme	different	672:680	arg1	CPSs					682:685	different CPSs	672:685	different CPSs	672:685	Indeed, the development of efficacious vaccine formulation is complicated by the high number of bacterial serotypes with different CPSs.					
34469105	10	74	theme	group	1595:1599	arg1	antisera					1604:1611	the group 19 antisera	1591:1611	the group 19 antisera	1591:1611	The disaccharide A-B, phosphorylated at the upstream end, emerged as a hit from the glycan array screening because it is strongly recognized by the group 19 antisera and by the 19F and 19A factor antisera, with similar intensity compared with the CPSs used as controls.					
34469105	0	75	theme	Capsular	58:65	arg1	Polysaccharides					67:81	the Capsular Polysaccharides	54:81	the Capsular Polysaccharides from Streptococcus pneumoniae	54:111	Glycan Array Evaluation of Synthetic Epitopes between the Capsular Polysaccharides from Streptococcus pneumoniae 19F and 19A.					
34469105	8	76	theme	different	1198:1206	arg1	combinations					1208:1219	different combinations	1198:1219	different combinations of the common 19F/19A disaccharide	1198:1254	Herein, we describe the synthesis of a small library of compounds containing different combinations of the common 19F/19A disaccharide.					
34469105	3	77	theme	vaccine	531:537	arg1	candidates					539:548	new vaccine candidates	527:548	new vaccine candidates	527:548	Protection is largely limited to the specific vaccine serotypes, and the continuous need for broader coverage to control the outbreak of emerging serotypes is pushing the development of new vaccine candidates.					
34469105	4	78	theme	high	632:635	arg1	number					637:642	the high number	628:642	the high number of bacterial serotypes with different CPSs	628:685	Indeed, the development of efficacious vaccine formulation is complicated by the high number of bacterial serotypes with different CPSs.					
32766842	4	0	theme	expression	504:513	arg1	system					515:520	an expression system	501:520	an expression system for the TNFR-Fc fusion protein (Etanercept)	501:564	Here, we constructed an expression system for the TNFR-Fc fusion protein (Etanercept) using transgenic silkworm.					
32766842	6	1	theme	plasmon	737:743	arg1	resonance					745:753	surface plasmon resonance	729:753	surface plasmon resonance analysis	729:762	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	6	2	theme	system	945:950	arg1	profit					924:929	the profit	920:929	the profit of expression system using transgenic silkworm	920:976	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	6	3	theme	expression	934:943	arg1	system					945:950	expression system	934:950	expression system using transgenic silkworm	934:976	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	5	4	theme	fusion	605:610	arg1	protein					612:618	The TNFR-Fc fusion protein	593:618	The TNFR-Fc fusion protein	593:618	The TNFR-Fc fusion protein was employed to N-glycan analysis, which revealed an increased amount of afucosylated protein.					
32766842	2	5	theme	glycosylation	341:353	arg1	patterns					355:362	low-cost, high-yield and unique glycosylation patterns	309:362	low-cost, high-yield and unique glycosylation patterns	309:362	Especially, transgenic silkworm has been studied intensively because of its ease in handling, low-cost, high-yield and unique glycosylation patterns.					
32766842	6	6	theme	Fcγ	842:844	arg1	receptor					846:853	Fcγ receptor IIIa and FcRn	842:867	receptor	846:853	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	5	7	theme	increased	673:681	arg1	amount					683:688	an increased amount	670:688	an increased amount of afucosylated protein	670:712	The TNFR-Fc fusion protein was employed to N-glycan analysis, which revealed an increased amount of afucosylated protein.					
32766842	5	7	theme	increased	673:681	arg1	protein					706:712	afucosylated protein	693:712	afucosylated protein	693:712	The TNFR-Fc fusion protein was employed to N-glycan analysis, which revealed an increased amount of afucosylated protein.					
32766842	6	8	theme	surface	729:735	arg1	resonance					745:753	surface plasmon resonance	729:753	surface plasmon resonance analysis	729:762	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	3	9	theme	transgenic	443:452	arg1	silkworm					454:461	transgenic silkworm	443:461	transgenic silkworm	443:461	However, the physicochemical property of the therapeutic protein expressed in transgenic silkworm remains elusive.					
32766842	0	10	theme	transgenic	90:99	arg1	silkworms					101:109	transgenic silkworms	90:109	transgenic silkworms	90:109	Structural insight and stability of TNFR-Fc fusion protein (Etanercept) produced by using transgenic silkworms.					
32766842	7	11	theme	thermal	1047:1053	arg1	stability					1055:1063	thermal stability	1047:1063	thermal stability	1047:1063	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	0	12	theme	TNFR-Fc	36:42	arg1	Etanercept					60:69	Etanercept	60:69	Etanercept	60:69	Structural insight and stability of TNFR-Fc fusion protein (Etanercept) produced by using transgenic silkworms.					
32766842	0	12	theme	TNFR-Fc	36:42	arg1	protein					51:57	TNFR-Fc fusion protein	36:57	TNFR-Fc fusion protein (Etanercept)	36:70	Structural insight and stability of TNFR-Fc fusion protein (Etanercept) produced by using transgenic silkworms.					
32766842	6	13	theme	binding	821:827	arg1	affinity					829:836	increased binding affinity	811:836	increased binding affinity for Fcγ receptor IIIa and FcRn	811:867	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	3	14	theme	physicochemical	378:392	arg1	property					394:401	the physicochemical property	374:401	the physicochemical property of the therapeutic protein expressed in transgenic silkworm	374:461	However, the physicochemical property of the therapeutic protein expressed in transgenic silkworm remains elusive.					
32766842	6	15	theme	increased	811:819	arg1	affinity					829:836	increased binding affinity	811:836	increased binding affinity for Fcγ receptor IIIa and FcRn	811:867	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	2	16	theme	low-cost	309:316	arg1	patterns					355:362	low-cost, high-yield and unique glycosylation patterns	309:362	low-cost, high-yield and unique glycosylation patterns	309:362	Especially, transgenic silkworm has been studied intensively because of its ease in handling, low-cost, high-yield and unique glycosylation patterns.					
32766842	1	17	theme	great	181:185	arg1	interest					187:194	great interest	181:194	great interest	181:194	Therapeutic proteins expressed using transgenic animals have been of great interest for several years.					
32766842	0	18	theme	Structural	0:9	arg1	insight					11:17	Structural insight	0:17	Structural insight	0:17	Structural insight and stability of TNFR-Fc fusion protein (Etanercept) produced by using transgenic silkworms.					
32766842	7	19	theme	protein	1103:1109	arg1	aggregation					1069:1079	aggregation	1069:1079	aggregation	1069:1079	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	7	19	theme	protein	1103:1109	arg1	structure					1036:1044	higher order structure	1023:1044	higher order structure	1023:1044	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	7	19	theme	protein	1103:1109	arg1	stability					1055:1063	thermal stability	1047:1063	thermal stability	1047:1063	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	5	20	theme	TNFR-Fc	597:603	arg1	protein					612:618	The TNFR-Fc fusion protein	593:618	The TNFR-Fc fusion protein	593:618	The TNFR-Fc fusion protein was employed to N-glycan analysis, which revealed an increased amount of afucosylated protein.					
32766842	7	21	theme	stability	1055:1063	arg1	comparison					1009:1018	the comparison	1005:1018	the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein	1005:1109	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	7	22	theme	aggregation	1069:1079	arg1	comparison					1009:1018	the comparison	1005:1018	the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein	1005:1109	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	4	23	theme	transgenic	572:581	arg1	silkworm					583:590	transgenic silkworm	572:590	transgenic silkworm	572:590	Here, we constructed an expression system for the TNFR-Fc fusion protein (Etanercept) using transgenic silkworm.					
32766842	5	24	gly	afucosylated	693:704	arg1	protein					706:712	afucosylated protein	693:712	afucosylated protein	693:712	The TNFR-Fc fusion protein was employed to N-glycan analysis, which revealed an increased amount of afucosylated protein.					
32766842	6	25	dep	receptor	846:853	arg1	IIIa					855:858	IIIa	855:858	IIIa	855:858	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	1	26	theme	several	200:206	arg1	years					208:212	several years	200:212	several years	200:212	Therapeutic proteins expressed using transgenic animals have been of great interest for several years.					
32766842	3	27	theme	therapeutic	410:420	arg1	protein					422:428	the therapeutic protein	406:428	the therapeutic protein expressed in transgenic silkworm	406:461	However, the physicochemical property of the therapeutic protein expressed in transgenic silkworm remains elusive.					
32766842	7	28	theme	TNFR-Fc	1088:1094	arg1	protein					1103:1109	the TNFR-Fc fusion protein	1084:1109	the TNFR-Fc fusion protein	1084:1109	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	6	29	theme	commercial	885:894	arg1	Etanercept					896:905	the commercial Etanercept	881:905	the commercial Etanercept	881:905	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	3	30	theme	protein	422:428	arg1	property					394:401	the physicochemical property	374:401	the physicochemical property of the therapeutic protein expressed in transgenic silkworm	374:461	However, the physicochemical property of the therapeutic protein expressed in transgenic silkworm remains elusive.					
32766842	4	31	theme	fusion	538:543	arg1	protein					545:551	the TNFR-Fc fusion protein	526:551	the TNFR-Fc fusion protein (Etanercept)	526:564	Here, we constructed an expression system for the TNFR-Fc fusion protein (Etanercept) using transgenic silkworm.					
32766842	4	31	theme	fusion	538:543	arg1	Etanercept					554:563	Etanercept	554:563	Etanercept	554:563	Here, we constructed an expression system for the TNFR-Fc fusion protein (Etanercept) using transgenic silkworm.					
32766842	7	32	theme	fusion	1096:1101	arg1	protein					1103:1109	the TNFR-Fc fusion protein	1084:1109	the TNFR-Fc fusion protein	1084:1109	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	2	33	theme	transgenic	227:236	arg1	silkworm					238:245	transgenic silkworm	227:245	transgenic silkworm	227:245	Especially, transgenic silkworm has been studied intensively because of its ease in handling, low-cost, high-yield and unique glycosylation patterns.					
32766842	6	34	theme	fusion	788:793	arg1	protein					795:801	the TNFR-Fc fusion protein	776:801	the TNFR-Fc fusion protein	776:801	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	0	35	theme	protein	51:57	arg1	stability					23:31	stability	23:31	stability	23:31	Structural insight and stability of TNFR-Fc fusion protein (Etanercept) produced by using transgenic silkworms.					
32766842	0	35	theme	protein	51:57	arg1	insight					11:17	Structural insight	0:17	Structural insight	0:17	Structural insight and stability of TNFR-Fc fusion protein (Etanercept) produced by using transgenic silkworms.					
32766842	4	36	theme	TNFR-Fc	530:536	arg1	protein					545:551	the TNFR-Fc fusion protein	526:551	the TNFR-Fc fusion protein (Etanercept)	526:564	Here, we constructed an expression system for the TNFR-Fc fusion protein (Etanercept) using transgenic silkworm.					
32766842	4	36	theme	TNFR-Fc	530:536	arg1	Etanercept					554:563	Etanercept	554:563	Etanercept	554:563	Here, we constructed an expression system for the TNFR-Fc fusion protein (Etanercept) using transgenic silkworm.					
32766842	6	37	theme	TNFR-Fc	780:786	arg1	protein					795:801	the TNFR-Fc fusion protein	776:801	the TNFR-Fc fusion protein	776:801	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	2	38	theme	unique	334:339	arg1	patterns					355:362	low-cost, high-yield and unique glycosylation patterns	309:362	low-cost, high-yield and unique glycosylation patterns	309:362	Especially, transgenic silkworm has been studied intensively because of its ease in handling, low-cost, high-yield and unique glycosylation patterns.					
32766842	0	39	theme	fusion	44:49	arg1	Etanercept					60:69	Etanercept	60:69	Etanercept	60:69	Structural insight and stability of TNFR-Fc fusion protein (Etanercept) produced by using transgenic silkworms.					
32766842	0	39	theme	fusion	44:49	arg1	protein					51:57	TNFR-Fc fusion protein	36:57	TNFR-Fc fusion protein (Etanercept)	36:70	Structural insight and stability of TNFR-Fc fusion protein (Etanercept) produced by using transgenic silkworms.					
32766842	5	40	theme	N-glycan	636:643	arg1	analysis					645:652	N-glycan analysis	636:652	N-glycan analysis	636:652	The TNFR-Fc fusion protein was employed to N-glycan analysis, which revealed an increased amount of afucosylated protein.					
32766842	7	41	theme	structure	1036:1044	arg1	comparison					1009:1018	the comparison	1005:1018	the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein	1005:1109	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	1	42	theme	Therapeutic	112:122	arg1	proteins					124:131	Therapeutic proteins	112:131	Therapeutic proteins expressed using transgenic animals	112:166	Therapeutic proteins expressed using transgenic animals have been of great interest for several years.					
32766842	6	43	theme	transgenic	958:967	arg1	silkworm					969:976	transgenic silkworm	958:976	transgenic silkworm	958:976	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	5	44	theme	afucosylated	693:704	arg1	protein					706:712	afucosylated protein	693:712	afucosylated protein	693:712	The TNFR-Fc fusion protein was employed to N-glycan analysis, which revealed an increased amount of afucosylated protein.					
32766842	2	45	theme	high-yield	319:328	arg1	patterns					355:362	low-cost, high-yield and unique glycosylation patterns	309:362	low-cost, high-yield and unique glycosylation patterns	309:362	Especially, transgenic silkworm has been studied intensively because of its ease in handling, low-cost, high-yield and unique glycosylation patterns.					
32766842	5	46	theme	protein	706:712	arg1	amount					683:688	an increased amount	670:688	an increased amount of afucosylated protein	670:712	The TNFR-Fc fusion protein was employed to N-glycan analysis, which revealed an increased amount of afucosylated protein.					
32766842	5	46	theme	protein	706:712	arg1	protein					706:712	afucosylated protein	693:712	afucosylated protein	693:712	The TNFR-Fc fusion protein was employed to N-glycan analysis, which revealed an increased amount of afucosylated protein.					
32766842	6	47	from	analysis	755:762	arg1	Evidence					715:722	Evidence	715:722	Evidence from surface plasmon resonance analysis	715:762	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	7	48	theme	higher	1023:1028	arg1	structure					1036:1044	higher order structure	1023:1044	higher order structure	1023:1044	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	2	49	from	ease	291:294	arg1	handling					299:306	handling	299:306	handling	299:306	Especially, transgenic silkworm has been studied intensively because of its ease in handling, low-cost, high-yield and unique glycosylation patterns.					
32766842	7	50	theme	order	1030:1034	arg1	structure					1036:1044	higher order structure	1023:1044	higher order structure	1023:1044	We have further discussed the comparison of higher order structure, thermal stability and aggregation of the TNFR-Fc fusion protein.					
32766842	6	51	theme	resonance	745:753	arg1	analysis					755:762	surface plasmon resonance analysis	729:762	surface plasmon resonance analysis	729:762	Evidence from surface plasmon resonance analysis showed that the TNFR-Fc fusion protein exhibit increased binding affinity for Fcγ receptor IIIa and FcRn compared to the commercial Etanercept, emphasizing the profit of expression system using transgenic silkworm.					
32766842	1	52	theme	transgenic	149:158	arg1	animals					160:166	transgenic animals	149:166	transgenic animals	149:166	Therapeutic proteins expressed using transgenic animals have been of great interest for several years.					
34213122	14	0	theme	UFH	2051:2053	arg1	digestion					2038:2046	exhaustive digestion	2027:2046	exhaustive digestion of UFH and LMWHs in a mixture of heparinases	2027:2091	The determination is based on the separation of building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases.					
34213122	26	1	theme	sulfation	4087:4095	arg1	degree					4097:4102	the sulfation degree	4083:4102	the sulfation degree of UFH	4083:4109	The obtained satisfactory data show that high fluctuation in the sulfation degree of UFH could transmit to the final enoxaparin products.					
34213122	28	2	theme	high	4361:4364	arg1	time					4406:4409	short analysis time	4391:4409	short analysis time	4391:4409	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	2	theme	high	4361:4364	arg1	automation					4379:4388	automation	4379:4388	automation	4379:4388	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	2	theme	high	4361:4364	arg1	sensitivity					4366:4376	high sensitivity	4361:4376	high sensitivity	4361:4376	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	3	theme	sample	4439:4444	arg1	time					4406:4409	short analysis time	4391:4409	short analysis time	4391:4409	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	3	theme	sample	4439:4444	arg1	consumption					4419:4429	low consumption	4415:4429	low consumption of both sample and reagents	4415:4457	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	3	theme	sample	4439:4444	arg1	automation					4379:4388	automation	4379:4388	automation	4379:4388	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	3	theme	sample	4439:4444	arg1	efficiency					4349:4358	high separation efficiency	4333:4358	high separation efficiency	4333:4358	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	3	theme	sample	4439:4444	arg1	sensitivity					4366:4376	high sensitivity	4361:4376	high sensitivity	4361:4376	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	18	4	theme	extinction	2640:2649	arg1	same					2707:2710	same	2707:2710	same	2707:2710	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	18	4	theme	extinction	2640:2649	arg1	coefficient					2651:2661	the molar extinction coefficient	2630:2661	the molar extinction coefficient of each oligosaccharide at UV 232 nm	2630:2698	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	2	5	theme	chemical	314:321	arg1	degradation					323:333	enzymatic or chemical degradation	301:333	enzymatic or chemical degradation	301:333	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	9	6	theme	sulfation	1397:1405	arg1	degree					1379:1384	the degree	1375:1384	the degree of heparin sulfation	1375:1405	In order to ensure the stability of product quality, it is necessary to develop a method for detecting the degree of heparin sulfation to monitor the stability of UFH and processing conditions.					
34213122	19	7	theme	degree	2994:2999	arg1	data					3001:3004	the sulfation degree data	2980:3004	the sulfation degree data between the enoxaparin batches from the different manufacturers	2980:3068	The developed method allows us to compare the sulfation degree data between the enoxaparin batches from the different manufacturers to evaluate the composition similarity.					
34213122	29	8	theme	good	4469:4472	arg1	potential					4486:4494	a good application potential	4467:4494	a good application potential	4467:4494	It has a good application potential in the quality control heparin production.					
34213122	19	9	theme	developed	2942:2950	arg1	method					2952:2957	The developed method	2938:2957	The developed method	2938:2957	The developed method allows us to compare the sulfation degree data between the enoxaparin batches from the different manufacturers to evaluate the composition similarity.					
34213122	3	10	theme	anticoagulant	525:537	arg1	effects					539:545	less bleeding, greater bioavailability, and more predictable anticoagulant effects	464:545	less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin	464:570	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	18	11	theme	ester	2839:2843	arg1	groups					2845:2850	sulfate ester groups	2831:2850	sulfate ester groups in each disaccharide unit	2831:2876	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	28	12	theme	low	4415:4417	arg1	consumption					4419:4429	low consumption	4415:4429	low consumption of both sample and reagents	4415:4457	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	15	13	theme	heparinase	2114:2123	arg1	Ⅰ					2125:2125	heparinase Ⅰ	2114:2125	heparinase Ⅰ	2114:2125	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ was prepared to give a final content of 0.13 IU/mL for each enzyme.					
34213122	12	14	theme	current	1764:1770	arg1	methods					1772:1778	the current methods	1760:1778	the current methods	1760:1778	These limitations make it difficult to apply the current methods to quality control via sulfation degree determination.					
34213122	28	15	theme	quantitative	4279:4290	arg1	analysis					4306:4313	quantitative compositional analysis	4279:4313	quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents	4279:4457	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	11	16	theme	reported	1636:1643	arg1	methods					1645:1651	the reported methods	1632:1651	the reported methods	1632:1651	However, the reported methods require expensive equipment and professional data processing.					
34213122	3	17	from	advantages	450:459	arg1	comparison					550:559	comparison	550:559	comparison to heparin	550:570	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	5	18	theme	molecular	795:803	arg1	weight					805:810	molecular weight	795:810	molecular weight	795:810	The structural characteristics of LMWHs differ from their parent heparin not only in terms of molecular weight but also in the sulfation degree as a result of losing the sulfate ester groups during the manufacturing process.					
34213122	22	19	theme	N-sulfation	3438:3448	arg1	data					3480:3483	O-sulfation (O-SD) data	3461:3483	O-sulfation (O-SD) data	3461:3483	The total sulfation degree (T-SD), the individual degree of N-sulfation (N-SD) and O-sulfation (O-SD) data were also determined and compared.					
34213122	22	19	theme	N-sulfation	3438:3448	arg1	T-SD					3406:3409	T-SD	3406:3409	T-SD	3406:3409	The total sulfation degree (T-SD), the individual degree of N-sulfation (N-SD) and O-sulfation (O-SD) data were also determined and compared.					
34213122	22	19	theme	N-sulfation	3438:3448	arg1	degree					3398:3403	The total sulfation degree	3378:3403	The total sulfation degree (T-SD)	3378:3410	The total sulfation degree (T-SD), the individual degree of N-sulfation (N-SD) and O-sulfation (O-SD) data were also determined and compared.					
34213122	22	19	theme	N-sulfation	3438:3448	arg1	degree					3428:3433	the individual degree	3413:3433	the individual degree of N-sulfation (N-SD)	3413:3455	The total sulfation degree (T-SD), the individual degree of N-sulfation (N-SD) and O-sulfation (O-SD) data were also determined and compared.					
34213122	28	20	theme	CE	4242:4243	arg1	method					4245:4250	The CE method	4238:4250	The CE method	4238:4250	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	16	21	theme	building	2366:2373	arg1	disaccharides					2408:2420	ten disaccharides	2404:2420	ten disaccharides	2404:2420	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	21	theme	building	2366:2373	arg1	derivatives					2487:2497	four 1,6-anhydro derivatives	2470:2497	four 1,6-anhydro derivatives	2470:2497	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	21	theme	building	2366:2373	arg1	tetrasaccharides					2448:2463	three tetrasaccharides	2442:2463	three tetrasaccharides	2442:2463	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	21	theme	building	2366:2373	arg1	trisaccharide					2427:2439	one trisaccharide	2423:2439	one trisaccharide	2423:2439	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	21	theme	building	2366:2373	arg1	blocks					2375:2380	building blocks	2366:2380	building blocks	2366:2380	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	8	22	theme	drug	1259:1262	arg1	safety					1264:1269	heparin drug safety	1251:1269	heparin drug safety	1251:1269	This will directly affect heparin drug safety.					
34213122	10	23	theme	structural	1577:1586	arg1	characterization					1588:1603	structural characterization	1577:1603	structural characterization of UFH and LMWHs	1577:1620	During the last two decades, various analytical methods based on chromatography or NMR have been developed for structural characterization of UFH and LMWHs.					
34213122	7	24	theme	wide	1073:1076	arg1	variation					1078:1086	a wide variation	1071:1086	a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process	1071:1222	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	9	25	theme	UFH	1435:1437	arg1	stability					1422:1430	the stability	1418:1430	the stability of UFH and processing conditions	1418:1463	In order to ensure the stability of product quality, it is necessary to develop a method for detecting the degree of heparin sulfation to monitor the stability of UFH and processing conditions.					
34213122	23	26	located	observed	3549:3556	arg1	SD					3565:3566	the SD	3561:3566	the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH	3561:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	23	26	located	observed	3549:3556	arg2	difference					3534:3543	A significant difference	3520:3543	A significant difference	3520:3543	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	23	27	theme	compositional	3689:3701	arg1	methods					3712:3718	the quantitative compositional analysis methods	3672:3718	the quantitative compositional analysis methods for quality control of LMWHs and UFH	3672:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	28	28	theme	LMWHs	4318:4322	arg1	analysis					4306:4313	quantitative compositional analysis	4279:4313	quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents	4279:4457	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	18	29	theme	peak	2793:2796	arg1	area					2798:2801	their peak area	2787:2801	their peak area	2787:2801	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	2	30	theme	unfractionated	269:282	arg1	heparin					284:290	the unfractionated heparin	265:290	the unfractionated heparin (UFH)	265:296	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	2	30	theme	unfractionated	269:282	arg1	UFH					293:295	UFH	293:295	UFH	293:295	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	9	31	theme	processing	1443:1452	arg1	stability					1422:1430	the stability	1418:1430	the stability of UFH and processing conditions	1418:1463	In order to ensure the stability of product quality, it is necessary to develop a method for detecting the degree of heparin sulfation to monitor the stability of UFH and processing conditions.					
34213122	6	32	theme	resulting	930:938	arg1	variation					954:962	The resulting compositional variation	926:962	The resulting compositional variation	926:962	The resulting compositional variation directly leads to a fluctuation in anticoagulant activity.					
34213122	5	33	theme	structural	705:714	arg1	characteristics					716:730	The structural characteristics	701:730	The structural characteristics of LMWHs	701:739	The structural characteristics of LMWHs differ from their parent heparin not only in terms of molecular weight but also in the sulfation degree as a result of losing the sulfate ester groups during the manufacturing process.					
34213122	5	34	theme	weight	805:810	arg1	terms					786:790	terms	786:790	terms of molecular weight	786:810	The structural characteristics of LMWHs differ from their parent heparin not only in terms of molecular weight but also in the sulfation degree as a result of losing the sulfate ester groups during the manufacturing process.					
34213122	5	35	theme	sulfation	828:836	arg1	degree					838:843	the sulfation degree	824:843	the sulfation degree as a result of losing the sulfate ester groups during the manufacturing process	824:923	The structural characteristics of LMWHs differ from their parent heparin not only in terms of molecular weight but also in the sulfation degree as a result of losing the sulfate ester groups during the manufacturing process.					
34213122	20	36	from	manufacturers	3178:3190	arg1	batches					3124:3130	eight batches	3118:3130	eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials	3118:3231	Herein, eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials were measured.					
34213122	17	37	theme	enoxaparin	2553:2562	arg1	compositions					2537:2548	the compositions	2533:2548	the compositions of enoxaparin and UFH	2533:2570	Then, the compositions of enoxaparin and UFH can be precisely determined.					
34213122	27	38	theme	products	4183:4190	arg1	consistency					4164:4174	The consistency	4160:4174	The consistency of the products	4160:4190	The consistency of the products can also be evaluated by using these methods.					
34213122	23	39	theme	different	3593:3601	arg1	manufacturers					3603:3615	the different manufacturers	3589:3615	the different manufacturers	3589:3615	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	28	40	contain	has	4252:4254	arg1	method					4245:4250	The CE method	4238:4250	The CE method	4238:4250	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	40	contain	has	4252:4254	arg2	advantages					4264:4273	several advantages	4256:4273	several advantages	4256:4273	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	15	41	theme	final	2160:2164	arg1	content					2166:2172	a final content	2158:2172	a final content of 0.13 IU/mL for each enzyme	2158:2202	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ was prepared to give a final content of 0.13 IU/mL for each enzyme.					
34213122	2	42	attach	derived	252:258	arg1	heparin					284:290	the unfractionated heparin	265:290	the unfractionated heparin (UFH)	265:296	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	2	42	attach	derived	252:258	arg2	LMWHs					241:245	LMWHs	241:245	LMWHs	241:245	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	2	42	attach	derived	252:258	arg2	heparins					231:238	Low molecular weight heparins	210:238	Low molecular weight heparins (LMWHs)	210:246	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	2	42	attach	derived	252:258	arg1	UFH					293:295	UFH	293:295	UFH	293:295	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	17	43	theme	UFH	2568:2570	arg1	compositions					2537:2548	the compositions	2533:2548	the compositions of enoxaparin and UFH	2533:2570	Then, the compositions of enoxaparin and UFH can be precisely determined.					
34213122	23	44	theme	significant	3522:3532	arg1	difference					3534:3543	A significant difference	3520:3543	A significant difference	3520:3543	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	23	45	theme	analysis	3703:3710	arg1	methods					3712:3718	the quantitative compositional analysis methods	3672:3718	the quantitative compositional analysis methods for quality control of LMWHs and UFH	3672:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	18	46	theme	average	2907:2913	arg1	degree					2925:2930	hence the average sulfation degree	2897:2930	hence the average sulfation degree (SD)	2897:2935	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	18	46	theme	average	2907:2913	arg1	SD					2933:2934	SD	2933:2934	SD	2933:2934	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	13	47	theme	degree	1915:1920	arg1	detection					1888:1896	the detection	1884:1896	the detection of the sulfation degree of UFH and LMWHs	1884:1937	Herein, we report a simple and robust method for the detection of the sulfation degree of UFH and LMWHs.					
34213122	0	48	theme	molecular	54:62	arg1	heparins					71:78	low molecular weight heparins	50:78	low molecular weight heparins	50:78	[Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis].					
34213122	24	49	theme	products	3819:3826	arg1	degree					3798:3803	the sulfation degree	3784:3803	the sulfation degree of enoxaparin products from different manufacturers	3784:3855	The variation in terms of the sulfation degree of enoxaparin products from different manufacturers can be precisely identified using this method.					
34213122	20	50	theme	materials	3223:3231	arg1	manufacturers					3178:3190	two manufacturers	3174:3190	two manufacturers	3174:3190	Herein, eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials were measured.					
34213122	20	50	theme	materials	3223:3231	arg1	batches					3201:3207	four batches	3196:3207	four batches	3196:3207	Herein, eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials were measured.					
34213122	18	51	from	groups	2845:2850	arg1	unit					2873:2876	each disaccharide unit	2855:2876	each disaccharide unit	2855:2876	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	18	52	from	unit	2873:2876	arg1	number					2821:2826	the accurate number	2808:2826	the accurate number of sulfate ester groups in each disaccharide unit	2808:2876	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	4	53	theme	used	690:693	arg1	LMWH					695:698	the most commonly used LMWH	672:698	the most commonly used LMWH	672:698	Enoxaparin, produced by the alkaline degradation of UFH through β-eliminative cleavage, represents the most commonly used LMWH.					
34213122	24	54	theme	sulfation	3788:3796	arg1	degree					3798:3803	the sulfation degree	3784:3803	the sulfation degree of enoxaparin products from different manufacturers	3784:3855	The variation in terms of the sulfation degree of enoxaparin products from different manufacturers can be precisely identified using this method.					
34213122	0	55	theme	heparins	71:78	arg1	degree					28:33	sulfation degree	18:33	sulfation degree of heparin and low molecular weight heparins	18:78	[Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis].					
34213122	1	56	theme	linear	152:157	arg1	saccharide					159:168	a highly sulfated linear saccharide	134:168	a highly sulfated linear saccharide	134:168	Heparin is composed of a highly sulfated linear saccharide and is widely used as an anticoagulant.					
34213122	14	57	theme	heparinases	2081:2091	arg1	mixture					2070:2076	a mixture	2068:2076	a mixture of heparinases	2068:2091	The determination is based on the separation of building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases.					
34213122	26	58	theme	final	4133:4137	arg1	products					4150:4157	the final enoxaparin products	4129:4157	the final enoxaparin products	4129:4157	The obtained satisfactory data show that high fluctuation in the sulfation degree of UFH could transmit to the final enoxaparin products.					
34213122	16	59	theme	samples	2245:2251	arg1	digestion					2209:2217	The digestion	2205:2217	The digestion of enoxaparin and heparin samples	2205:2251	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	0	60	theme	capillary	83:91	arg1	electrophoresis					93:107	capillary electrophoresis	83:107	capillary electrophoresis	83:107	[Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis].					
34213122	28	61	theme	several	4256:4262	arg1	advantages					4264:4273	several advantages	4256:4273	several advantages	4256:4273	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	14	62	theme	blocks	1997:2002	arg1	separation					1974:1983	the separation	1970:1983	the separation of building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases	1970:2091	The determination is based on the separation of building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases.					
34213122	13	63	theme	simple	1855:1860	arg1	method					1873:1878	a simple and robust method	1853:1878	a simple and robust method for the detection of the sulfation degree of UFH and LMWHs	1853:1937	Herein, we report a simple and robust method for the detection of the sulfation degree of UFH and LMWHs.					
34213122	26	64	from	fluctuation	4068:4078	arg1	degree					4097:4102	the sulfation degree	4083:4102	the sulfation degree of UFH	4083:4109	The obtained satisfactory data show that high fluctuation in the sulfation degree of UFH could transmit to the final enoxaparin products.					
34213122	9	65	theme	quality	1316:1322	arg1	stability					1295:1303	the stability	1291:1303	the stability of product quality	1291:1322	In order to ensure the stability of product quality, it is necessary to develop a method for detecting the degree of heparin sulfation to monitor the stability of UFH and processing conditions.					
34213122	6	66	from	fluctuation	984:994	arg1	activity					1013:1020	anticoagulant activity	999:1020	anticoagulant activity	999:1020	The resulting compositional variation directly leads to a fluctuation in anticoagulant activity.					
34213122	25	67	theme	detailed	3936:3943	arg1	differences					3959:3969	the detailed compositional differences	3932:3969	the detailed compositional differences between products from the different manufacturers	3932:4019	This allows us to determine the detailed compositional differences between products from the different manufacturers.					
34213122	13	68	theme	robust	1866:1871	arg1	method					1873:1878	a simple and robust method	1853:1878	a simple and robust method for the detection of the sulfation degree of UFH and LMWHs	1853:1937	Herein, we report a simple and robust method for the detection of the sulfation degree of UFH and LMWHs.					
34213122	7	69	theme	slight	1176:1181	arg1	differences					1183:1193	slight differences	1176:1193	slight differences in the manufacturing process	1176:1222	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	24	70	theme	degree	3798:3803	arg1	terms					3775:3779	terms	3775:3779	terms of the sulfation degree of enoxaparin products from different manufacturers	3775:3855	The variation in terms of the sulfation degree of enoxaparin products from different manufacturers can be precisely identified using this method.					
34213122	18	71	from	number	2821:2826	arg1	unit					2873:2876	each disaccharide unit	2855:2876	each disaccharide unit	2855:2876	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	0	72	theme	sulfation	18:26	arg1	degree					28:33	sulfation degree	18:33	sulfation degree of heparin and low molecular weight heparins	18:78	[Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis].					
34213122	16	73	theme	heparin	2385:2391	arg1	disaccharides					2408:2420	ten disaccharides	2404:2420	ten disaccharides	2404:2420	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	73	theme	heparin	2385:2391	arg1	derivatives					2487:2497	four 1,6-anhydro derivatives	2470:2497	four 1,6-anhydro derivatives	2470:2497	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	73	theme	heparin	2385:2391	arg1	tetrasaccharides					2448:2463	three tetrasaccharides	2442:2463	three tetrasaccharides	2442:2463	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	73	theme	heparin	2385:2391	arg1	trisaccharide					2427:2439	one trisaccharide	2423:2439	one trisaccharide	2423:2439	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	73	theme	heparin	2385:2391	arg1	blocks					2375:2380	building blocks	2366:2380	building blocks	2366:2380	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	29	74	contain	has	4463:4465	arg2	potential					4486:4494	a good application potential	4467:4494	a good application potential	4467:4494	It has a good application potential in the quality control heparin production.					
34213122	29	74	contain	has	4463:4465	arg1	It					4460:4461	It	4460:4461	It	4460:4461	It has a good application potential in the quality control heparin production.					
34213122	21	75	theme	average	3296:3302	arg1	values					3304:3309	the average values	3292:3309	the average values as well as the relative standard deviations (RSD)	3292:3359	Each sample was measured in triplicate, and the average values as well as the relative standard deviations (RSD) were calculated.					
34213122	11	76	theme	data	1698:1701	arg1	processing					1703:1712	professional data processing	1685:1712	professional data processing	1685:1712	However, the reported methods require expensive equipment and professional data processing.					
34213122	19	77	theme	composition	3086:3096	arg1	similarity					3098:3107	the composition similarity	3082:3107	the composition similarity	3082:3107	The developed method allows us to compare the sulfation degree data between the enoxaparin batches from the different manufacturers to evaluate the composition similarity.					
34213122	3	78	theme	thrombosis	424:433	arg1	prevention					410:419	prevention	410:419	prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin	410:570	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	3	78	theme	thrombosis	424:433	arg1	treatment					396:404	treatment	396:404	treatment	396:404	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	7	79	theme	different	1143:1151	arg1	manufacturers					1153:1165	different manufacturers	1143:1165	different manufacturers owing to slight differences in the manufacturing process	1143:1222	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	12	80	theme	degree	1813:1818	arg1	determination					1820:1832	sulfation degree determination	1803:1832	sulfation degree determination	1803:1832	These limitations make it difficult to apply the current methods to quality control via sulfation degree determination.					
34213122	28	81	theme	high	4333:4336	arg1	efficiency					4349:4358	high separation efficiency	4333:4358	high separation efficiency	4333:4358	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	29	82	theme	heparin	4519:4525	arg1	production					4527:4536	the quality control heparin production	4499:4536	the quality control heparin production	4499:4536	It has a good application potential in the quality control heparin production.					
34213122	10	83	theme	analytical	1503:1512	arg1	methods					1514:1520	various analytical methods	1495:1520	various analytical methods based on chromatography or NMR	1495:1551	During the last two decades, various analytical methods based on chromatography or NMR have been developed for structural characterization of UFH and LMWHs.					
34213122	21	84	theme	standard	3335:3342	arg1	RSD					3356:3358	RSD	3356:3358	RSD	3356:3358	Each sample was measured in triplicate, and the average values as well as the relative standard deviations (RSD) were calculated.					
34213122	21	84	theme	standard	3335:3342	arg1	deviations					3344:3353	the relative standard deviations	3322:3353	the average values as well as the relative standard deviations (RSD)	3292:3359	Each sample was measured in triplicate, and the average values as well as the relative standard deviations (RSD) were calculated.					
34213122	26	85	theme	obtained	4026:4033	arg1	data					4048:4051	The obtained satisfactory data	4022:4051	The obtained satisfactory data	4022:4051	The obtained satisfactory data show that high fluctuation in the sulfation degree of UFH could transmit to the final enoxaparin products.					
34213122	24	86	from	degree	3798:3803	arg1	manufacturers					3843:3855	different manufacturers	3833:3855	different manufacturers	3833:3855	The variation in terms of the sulfation degree of enoxaparin products from different manufacturers can be precisely identified using this method.					
34213122	20	87	theme	enoxaparin	3158:3167	arg1	batches					3124:3130	eight batches	3118:3130	eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials	3118:3231	Herein, eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials were measured.					
34213122	26	88	theme	high	4063:4066	arg1	fluctuation					4068:4078	high fluctuation	4063:4078	high fluctuation in the sulfation degree of UFH	4063:4109	The obtained satisfactory data show that high fluctuation in the sulfation degree of UFH could transmit to the final enoxaparin products.					
34213122	18	89	theme	oligosaccharide	2671:2685	arg1	same					2707:2710	same	2707:2710	same	2707:2710	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	18	89	theme	oligosaccharide	2671:2685	arg1	coefficient					2651:2661	the molar extinction coefficient	2630:2661	the molar extinction coefficient of each oligosaccharide at UV 232 nm	2630:2698	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	16	90	theme	capillary	2296:2304	arg1	CE					2323:2324	CE	2323:2324	CE	2323:2324	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	90	theme	capillary	2296:2304	arg1	electrophoresis					2306:2320	a capillary electrophoresis	2294:2320	a capillary electrophoresis (CE) method	2294:2332	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	18	91	theme	sulfate	2831:2837	arg1	groups					2845:2850	sulfate ester groups	2831:2850	sulfate ester groups in each disaccharide unit	2831:2876	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	19	92	from	manufacturers	3056:3068	arg1	data					3001:3004	the sulfation degree data	2980:3004	the sulfation degree data between the enoxaparin batches from the different manufacturers	2980:3068	The developed method allows us to compare the sulfation degree data between the enoxaparin batches from the different manufacturers to evaluate the composition similarity.					
34213122	12	93	theme	quality	1783:1789	arg1	control					1791:1797	quality control	1783:1797	quality control	1783:1797	These limitations make it difficult to apply the current methods to quality control via sulfation degree determination.					
34213122	3	94	theme	bioavailability	487:501	arg1	effects					539:545	less bleeding, greater bioavailability, and more predictable anticoagulant effects	464:545	less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin	464:570	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	0	95	theme	low	50:52	arg1	heparins					71:78	low molecular weight heparins	50:78	low molecular weight heparins	50:78	[Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis].					
34213122	25	96	theme	different	3997:4005	arg1	manufacturers					4007:4019	the different manufacturers	3993:4019	the different manufacturers	3993:4019	This allows us to determine the detailed compositional differences between products from the different manufacturers.					
34213122	29	97	theme	quality	4503:4509	arg1	production					4527:4536	the quality control heparin production	4499:4536	the quality control heparin production	4499:4536	It has a good application potential in the quality control heparin production.					
34213122	26	98	theme	UFH	4107:4109	arg1	degree					4097:4102	the sulfation degree	4083:4102	the sulfation degree of UFH	4083:4109	The obtained satisfactory data show that high fluctuation in the sulfation degree of UFH could transmit to the final enoxaparin products.					
34213122	14	99	theme	exhaustive	2027:2036	arg1	digestion					2038:2046	exhaustive digestion	2027:2046	exhaustive digestion of UFH and LMWHs in a mixture of heparinases	2027:2091	The determination is based on the separation of building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases.					
34213122	9	100	theme	heparin	1389:1395	arg1	sulfation					1397:1405	heparin sulfation	1389:1405	heparin sulfation	1389:1405	In order to ensure the stability of product quality, it is necessary to develop a method for detecting the degree of heparin sulfation to monitor the stability of UFH and processing conditions.					
34213122	22	101	theme	O-sulfation	3461:3471	arg1	data					3480:3483	O-sulfation (O-SD) data	3461:3483	O-sulfation (O-SD) data	3461:3483	The total sulfation degree (T-SD), the individual degree of N-sulfation (N-SD) and O-sulfation (O-SD) data were also determined and compared.					
34213122	28	102	theme	reagents	4450:4457	arg1	time					4406:4409	short analysis time	4391:4409	short analysis time	4391:4409	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	102	theme	reagents	4450:4457	arg1	consumption					4419:4429	low consumption	4415:4429	low consumption of both sample and reagents	4415:4457	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	102	theme	reagents	4450:4457	arg1	automation					4379:4388	automation	4379:4388	automation	4379:4388	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	102	theme	reagents	4450:4457	arg1	efficiency					4349:4358	high separation efficiency	4333:4358	high separation efficiency	4333:4358	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	102	theme	reagents	4450:4457	arg1	sensitivity					4366:4376	high sensitivity	4361:4376	high sensitivity	4361:4376	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	7	103	dep	In	1023:1024	arg1	vitro					1026:1030	vitro	1026:1030	vitro	1026:1030	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	19	104	theme	sulfation	2984:2992	arg1	data					3001:3004	the sulfation degree data	2980:3004	the sulfation degree data between the enoxaparin batches from the different manufacturers	2980:3068	The developed method allows us to compare the sulfation degree data between the enoxaparin batches from the different manufacturers to evaluate the composition similarity.					
34213122	7	105	theme	anticoagulant	1091:1103	arg1	activity					1105:1112	anticoagulant activity	1091:1112	anticoagulant activity	1091:1112	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	18	106	theme	molar	2634:2638	arg1	same					2707:2710	same	2707:2710	same	2707:2710	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	18	106	theme	molar	2634:2638	arg1	coefficient					2651:2661	the molar extinction coefficient	2630:2661	the molar extinction coefficient of each oligosaccharide at UV 232 nm	2630:2698	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	3	107	theme	predictable	513:523	arg1	effects					539:545	less bleeding, greater bioavailability, and more predictable anticoagulant effects	464:545	less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin	464:570	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	18	108	theme	groups	2845:2850	arg1	number					2821:2826	the accurate number	2808:2826	the accurate number of sulfate ester groups in each disaccharide unit	2808:2876	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	16	109	theme	oligosaccharides	2349:2364	arg1	total					2337:2341	a total	2335:2341	a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated	2335:2524	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	109	theme	oligosaccharides	2349:2364	arg1	method					2327:2332	a capillary electrophoresis (CE) method	2294:2332	a capillary electrophoresis (CE) method	2294:2332	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	23	110	theme	UFH	3753:3755	arg1	control					3732:3738	quality control	3724:3738	quality control of LMWHs and UFH	3724:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	20	111	theme	UFH	3212:3214	arg1	materials					3223:3231	UFH source materials	3212:3231	UFH source materials	3212:3231	Herein, eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials were measured.					
34213122	23	112	from	manufacturers	3603:3615	arg1	SD					3565:3566	the SD	3561:3566	the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH	3561:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	23	112	from	manufacturers	3603:3615	arg1	products					3575:3582	the products	3571:3582	the products	3571:3582	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	28	113	theme	separation	4338:4347	arg1	efficiency					4349:4358	high separation efficiency	4333:4358	high separation efficiency	4333:4358	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	11	114	theme	expensive	1661:1669	arg1	equipment					1671:1679	expensive equipment	1661:1679	expensive equipment	1661:1679	However, the reported methods require expensive equipment and professional data processing.					
34213122	23	115	theme	quantitative	3676:3687	arg1	methods					3712:3718	the quantitative compositional analysis methods	3672:3718	the quantitative compositional analysis methods for quality control of LMWHs and UFH	3672:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	18	116	from	nm	2697:2698	arg1	same					2707:2710	same	2707:2710	same	2707:2710	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	18	116	from	nm	2697:2698	arg1	coefficient					2651:2661	the molar extinction coefficient	2630:2661	the molar extinction coefficient of each oligosaccharide at UV 232 nm	2630:2698	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	15	117	theme	Ⅱ	2128:2128	arg1	solution					2102:2109	A mixed solution	2094:2109	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ	2094:2135	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ was prepared to give a final content of 0.13 IU/mL for each enzyme.					
34213122	25	118	from	manufacturers	4007:4019	arg1	products					3979:3986	products	3979:3986	products from the different manufacturers	3979:4019	This allows us to determine the detailed compositional differences between products from the different manufacturers.					
34213122	4	119	theme	β-eliminative	637:649	arg1	cleavage					651:658	β-eliminative cleavage	637:658	β-eliminative cleavage	637:658	Enoxaparin, produced by the alkaline degradation of UFH through β-eliminative cleavage, represents the most commonly used LMWH.					
34213122	13	120	theme	UFH	1925:1927	arg1	degree					1915:1920	the sulfation degree	1901:1920	the sulfation degree of UFH and LMWHs	1901:1937	Herein, we report a simple and robust method for the detection of the sulfation degree of UFH and LMWHs.					
34213122	23	121	theme	products	3575:3582	arg1	SD					3565:3566	the SD	3561:3566	the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH	3561:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	15	122	theme	Ⅰ	2125:2125	arg1	solution					2102:2109	A mixed solution	2094:2109	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ	2094:2135	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ was prepared to give a final content of 0.13 IU/mL for each enzyme.					
34213122	8	123	theme	heparin	1251:1257	arg1	safety					1264:1269	heparin drug safety	1251:1269	heparin drug safety	1251:1269	This will directly affect heparin drug safety.					
34213122	13	124	theme	LMWHs	1933:1937	arg1	degree					1915:1920	the sulfation degree	1901:1920	the sulfation degree of UFH and LMWHs	1901:1937	Herein, we report a simple and robust method for the detection of the sulfation degree of UFH and LMWHs.					
34213122	3	125	theme	effects	539:545	arg1	advantages					450:459	the advantages	446:459	the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin	446:570	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	6	126	theme	compositional	940:952	arg1	variation					954:962	The resulting compositional variation	926:962	The resulting compositional variation	926:962	The resulting compositional variation directly leads to a fluctuation in anticoagulant activity.					
34213122	18	127	theme	UV	2690:2691	arg1	nm					2697:2698	UV 232 nm	2690:2698	UV 232 nm	2690:2698	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	7	128	from	differences	1183:1193	arg1	process					1216:1222	the manufacturing process	1198:1222	the manufacturing process	1198:1222	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	5	129	theme	sulfate	871:877	arg1	groups					885:890	the sulfate ester groups	867:890	the sulfate ester groups	867:890	The structural characteristics of LMWHs differ from their parent heparin not only in terms of molecular weight but also in the sulfation degree as a result of losing the sulfate ester groups during the manufacturing process.					
34213122	24	130	from	variation	3762:3770	arg1	terms					3775:3779	terms	3775:3779	terms of the sulfation degree of enoxaparin products from different manufacturers	3775:3855	The variation in terms of the sulfation degree of enoxaparin products from different manufacturers can be precisely identified using this method.					
34213122	10	131	theme	LMWHs	1616:1620	arg1	characterization					1588:1603	structural characterization	1577:1603	structural characterization of UFH and LMWHs	1577:1620	During the last two decades, various analytical methods based on chromatography or NMR have been developed for structural characterization of UFH and LMWHs.					
34213122	2	132	theme	enzymatic	301:309	arg1	degradation					323:333	enzymatic or chemical degradation	301:333	enzymatic or chemical degradation	301:333	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	22	133	theme	individual	3417:3426	arg1	degree					3428:3433	the individual degree	3413:3433	the individual degree of N-sulfation (N-SD)	3413:3455	The total sulfation degree (T-SD), the individual degree of N-sulfation (N-SD) and O-sulfation (O-SD) data were also determined and compared.					
34213122	2	134	theme	weight	224:229	arg1	LMWHs					241:245	LMWHs	241:245	LMWHs	241:245	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	2	134	theme	weight	224:229	arg1	heparins					231:238	Low molecular weight heparins	210:238	Low molecular weight heparins (LMWHs)	210:246	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	15	135	theme	mixed	2096:2100	arg1	solution					2102:2109	A mixed solution	2094:2109	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ	2094:2135	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ was prepared to give a final content of 0.13 IU/mL for each enzyme.					
34213122	4	136	theme	UFH	625:627	arg1	degradation					610:620	the alkaline degradation	597:620	the alkaline degradation of UFH	597:627	Enoxaparin, produced by the alkaline degradation of UFH through β-eliminative cleavage, represents the most commonly used LMWH.					
34213122	10	137	theme	UFH	1608:1610	arg1	characterization					1588:1603	structural characterization	1577:1603	structural characterization of UFH and LMWHs	1577:1620	During the last two decades, various analytical methods based on chromatography or NMR have been developed for structural characterization of UFH and LMWHs.					
34213122	7	138	theme	functional	1032:1041	arg1	assays					1043:1048	In vitro functional assays	1023:1048	In vitro functional assays	1023:1048	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	2	139	theme	Low	210:212	arg1	LMWHs					241:245	LMWHs	241:245	LMWHs	241:245	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	2	139	theme	Low	210:212	arg1	heparins					231:238	Low molecular weight heparins	210:238	Low molecular weight heparins (LMWHs)	210:246	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	28	140	theme	short	4391:4395	arg1	time					4406:4409	short analysis time	4391:4409	short analysis time	4391:4409	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	140	theme	short	4391:4395	arg1	sensitivity					4366:4376	high sensitivity	4361:4376	high sensitivity	4361:4376	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	5	141	theme	ester	879:883	arg1	groups					885:890	the sulfate ester groups	867:890	the sulfate ester groups	867:890	The structural characteristics of LMWHs differ from their parent heparin not only in terms of molecular weight but also in the sulfation degree as a result of losing the sulfate ester groups during the manufacturing process.					
34213122	1	142	used	used	184:187	arg2	anticoagulant					195:207	an anticoagulant	192:207	an anticoagulant	192:207	Heparin is composed of a highly sulfated linear saccharide and is widely used as an anticoagulant.					
34213122	1	142	used	used	184:187	arg2	Heparin					111:117	Heparin	111:117	Heparin	111:117	Heparin is composed of a highly sulfated linear saccharide and is widely used as an anticoagulant.					
34213122	3	143	from	effects	539:545	arg1	comparison					550:559	comparison	550:559	comparison to heparin	550:570	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	7	144	theme	In	1023:1024	arg1	assays					1043:1048	In vitro functional assays	1023:1048	In vitro functional assays	1023:1048	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	18	145	theme	sulfation	2915:2923	arg1	degree					2925:2930	hence the average sulfation degree	2897:2930	hence the average sulfation degree (SD)	2897:2935	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	18	145	theme	sulfation	2915:2923	arg1	SD					2933:2934	SD	2933:2934	SD	2933:2934	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	20	146	theme	available	3148:3156	arg1	enoxaparin					3158:3167	commercially available enoxaparin	3135:3167	commercially available enoxaparin	3135:3167	Herein, eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials were measured.					
34213122	13	147	theme	sulfation	1905:1913	arg1	degree					1915:1920	the sulfation degree	1901:1920	the sulfation degree of UFH and LMWHs	1901:1937	Herein, we report a simple and robust method for the detection of the sulfation degree of UFH and LMWHs.					
34213122	16	148	theme	enoxaparin	2222:2231	arg1	digestion					2209:2217	The digestion	2205:2217	The digestion of enoxaparin and heparin samples	2205:2251	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	0	149	theme	weight	64:69	arg1	heparins					71:78	low molecular weight heparins	50:78	low molecular weight heparins	50:78	[Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis].					
34213122	6	150	theme	anticoagulant	999:1011	arg1	activity					1013:1020	anticoagulant activity	999:1020	anticoagulant activity	999:1020	The resulting compositional variation directly leads to a fluctuation in anticoagulant activity.					
34213122	22	151	theme	sulfation	3388:3396	arg1	T-SD					3406:3409	T-SD	3406:3409	T-SD	3406:3409	The total sulfation degree (T-SD), the individual degree of N-sulfation (N-SD) and O-sulfation (O-SD) data were also determined and compared.					
34213122	22	151	theme	sulfation	3388:3396	arg1	degree					3398:3403	The total sulfation degree	3378:3403	The total sulfation degree (T-SD)	3378:3410	The total sulfation degree (T-SD), the individual degree of N-sulfation (N-SD) and O-sulfation (O-SD) data were also determined and compared.					
34213122	16	152	theme	heparin	2237:2243	arg1	samples					2245:2251	heparin samples	2237:2251	heparin samples	2237:2251	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	7	153	theme	manufacturing	1202:1214	arg1	process					1216:1222	the manufacturing process	1198:1222	the manufacturing process	1198:1222	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	5	154	theme	parent	759:764	arg1	heparin					766:772	their parent heparin	753:772	their parent heparin	753:772	The structural characteristics of LMWHs differ from their parent heparin not only in terms of molecular weight but also in the sulfation degree as a result of losing the sulfate ester groups during the manufacturing process.					
34213122	20	155	from	batches	3201:3207	arg1	batches					3124:3130	eight batches	3118:3130	eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials	3118:3231	Herein, eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials were measured.					
34213122	28	156	theme	analysis	4397:4404	arg1	time					4406:4409	short analysis time	4391:4409	short analysis time	4391:4409	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	28	156	theme	analysis	4397:4404	arg1	sensitivity					4366:4376	high sensitivity	4361:4376	high sensitivity	4361:4376	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	1	157	theme	sulfated	143:150	arg1	saccharide					159:168	a highly sulfated linear saccharide	134:168	a highly sulfated linear saccharide	134:168	Heparin is composed of a highly sulfated linear saccharide and is widely used as an anticoagulant.					
34213122	20	158	theme	source	3216:3221	arg1	materials					3223:3231	UFH source materials	3212:3231	UFH source materials	3212:3231	Herein, eight batches of commercially available enoxaparin from two manufacturers and four batches of UFH source materials were measured.					
34213122	19	159	theme	enoxaparin	3018:3027	arg1	batches					3029:3035	the enoxaparin batches	3014:3035	the enoxaparin batches	3014:3035	The developed method allows us to compare the sulfation degree data between the enoxaparin batches from the different manufacturers to evaluate the composition similarity.					
34213122	24	160	from	manufacturers	3843:3855	arg1	degree					3798:3803	the sulfation degree	3784:3803	the sulfation degree of enoxaparin products from different manufacturers	3784:3855	The variation in terms of the sulfation degree of enoxaparin products from different manufacturers can be precisely identified using this method.					
34213122	24	160	from	manufacturers	3843:3855	arg1	products					3819:3826	enoxaparin products	3808:3826	enoxaparin products from different manufacturers	3808:3855	The variation in terms of the sulfation degree of enoxaparin products from different manufacturers can be precisely identified using this method.					
34213122	29	161	theme	control	4511:4517	arg1	production					4527:4536	the quality control heparin production	4499:4536	the quality control heparin production	4499:4536	It has a good application potential in the quality control heparin production.					
34213122	16	162	theme	1,6-anhydro	2475:2485	arg1	derivatives					2487:2497	four 1,6-anhydro derivatives	2470:2497	four 1,6-anhydro derivatives	2470:2497	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	7	163	from	manufacturers	1153:1165	arg1	LMWHs					1132:1136	the various LMWHs	1120:1136	the various LMWHs from different manufacturers owing to slight differences in the manufacturing process	1120:1222	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	15	164	theme	Ⅲ	2135:2135	arg1	solution					2102:2109	A mixed solution	2094:2109	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ	2094:2135	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ was prepared to give a final content of 0.13 IU/mL for each enzyme.					
34213122	5	165	theme	LMWHs	735:739	arg1	characteristics					716:730	The structural characteristics	701:730	The structural characteristics of LMWHs	701:739	The structural characteristics of LMWHs differ from their parent heparin not only in terms of molecular weight but also in the sulfation degree as a result of losing the sulfate ester groups during the manufacturing process.					
34213122	9	166	theme	product	1308:1314	arg1	quality					1316:1322	product quality	1308:1322	product quality	1308:1322	In order to ensure the stability of product quality, it is necessary to develop a method for detecting the degree of heparin sulfation to monitor the stability of UFH and processing conditions.					
34213122	3	167	from	comparison	550:559	arg1	advantages					450:459	the advantages	446:459	the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin	446:570	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	14	168	theme	building	1988:1995	arg1	blocks					1997:2002	building blocks	1988:2002	building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases	1988:2091	The determination is based on the separation of building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases.					
34213122	19	169	theme	different	3046:3054	arg1	manufacturers					3056:3068	the different manufacturers	3042:3068	the different manufacturers	3042:3068	The developed method allows us to compare the sulfation degree data between the enoxaparin batches from the different manufacturers to evaluate the composition similarity.					
34213122	18	170	theme	oligosaccharide	2739:2753	arg1	concentration					2717:2729	the concentration	2713:2729	the concentration of each oligosaccharide	2713:2753	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	5	171	theme	manufacturing	903:915	arg1	process					917:923	the manufacturing process	899:923	the manufacturing process	899:923	The structural characteristics of LMWHs differ from their parent heparin not only in terms of molecular weight but also in the sulfation degree as a result of losing the sulfate ester groups during the manufacturing process.					
34213122	16	172	dep	total	2337:2341	arg1	baseline					2507:2514	baseline	2507:2514	baseline separated	2507:2524	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	14	173	theme	LMWHs	2059:2063	arg1	digestion					2038:2046	exhaustive digestion	2027:2046	exhaustive digestion of UFH and LMWHs in a mixture of heparinases	2027:2091	The determination is based on the separation of building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases.					
34213122	25	174	theme	compositional	3945:3957	arg1	differences					3959:3969	the detailed compositional differences	3932:3969	the detailed compositional differences between products from the different manufacturers	3932:4019	This allows us to determine the detailed compositional differences between products from the different manufacturers.					
34213122	24	175	theme	different	3833:3841	arg1	manufacturers					3843:3855	different manufacturers	3833:3855	different manufacturers	3833:3855	The variation in terms of the sulfation degree of enoxaparin products from different manufacturers can be precisely identified using this method.					
34213122	0	176	theme	degree	28:33	arg1	[Determination					0:13	[Determination	0:13	[Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis	0:107	[Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis].					
34213122	3	177	dep	bioavailability	487:501	arg1	greater					479:485	greater	479:485	greater	479:485	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	7	178	from	variation	1078:1086	arg1	activity					1105:1112	anticoagulant activity	1091:1112	anticoagulant activity	1091:1112	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	10	179	theme	last	1477:1480	arg1	decades					1486:1492	the last two decades	1473:1492	the last two decades	1473:1492	During the last two decades, various analytical methods based on chromatography or NMR have been developed for structural characterization of UFH and LMWHs.					
34213122	11	180	theme	professional	1685:1696	arg1	processing					1703:1712	professional data processing	1685:1712	professional data processing	1685:1712	However, the reported methods require expensive equipment and professional data processing.					
34213122	0	181	theme	heparin	38:44	arg1	degree					28:33	sulfation degree	18:33	sulfation degree of heparin and low molecular weight heparins	18:78	[Determination of sulfation degree of heparin and low molecular weight heparins by capillary electrophoresis].					
34213122	18	182	theme	disaccharide	2860:2871	arg1	unit					2873:2876	each disaccharide unit	2855:2876	each disaccharide unit	2855:2876	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34213122	14	183	from	digestion	2038:2046	arg1	mixture					2070:2076	a mixture	2068:2076	a mixture of heparinases	2068:2091	The determination is based on the separation of building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases.					
34213122	26	184	theme	enoxaparin	4139:4148	arg1	products					4150:4157	the final enoxaparin products	4129:4157	the final enoxaparin products	4129:4157	The obtained satisfactory data show that high fluctuation in the sulfation degree of UFH could transmit to the final enoxaparin products.					
34213122	23	185	theme	LMWHs	3743:3747	arg1	control					3732:3738	quality control	3724:3738	quality control of LMWHs and UFH	3724:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	29	186	theme	application	4474:4484	arg1	potential					4486:4494	a good application potential	4467:4494	a good application potential	4467:4494	It has a good application potential in the quality control heparin production.					
34213122	9	187	dep	UFH	1435:1437	arg1	conditions					1454:1463	conditions	1454:1463	conditions	1454:1463	In order to ensure the stability of product quality, it is necessary to develop a method for detecting the degree of heparin sulfation to monitor the stability of UFH and processing conditions.					
34213122	21	188	theme	relative	3326:3333	arg1	RSD					3356:3358	RSD	3356:3358	RSD	3356:3358	Each sample was measured in triplicate, and the average values as well as the relative standard deviations (RSD) were calculated.					
34213122	21	188	theme	relative	3326:3333	arg1	deviations					3344:3353	the relative standard deviations	3322:3353	the average values as well as the relative standard deviations (RSD)	3292:3359	Each sample was measured in triplicate, and the average values as well as the relative standard deviations (RSD) were calculated.					
34213122	7	189	theme	various	1124:1130	arg1	LMWHs					1132:1136	the various LMWHs	1120:1136	the various LMWHs from different manufacturers owing to slight differences in the manufacturing process	1120:1222	In vitro functional assays showed that there is a wide variation in anticoagulant activity among the various LMWHs from different manufacturers owing to slight differences in the manufacturing process.					
34213122	15	190	theme	IU/mL	2182:2186	arg1	content					2166:2172	a final content	2158:2172	a final content of 0.13 IU/mL for each enzyme	2158:2202	A mixed solution of heparinase Ⅰ, Ⅱ, and Ⅲ was prepared to give a final content of 0.13 IU/mL for each enzyme.					
34213122	12	191	theme	sulfation	1803:1811	arg1	determination					1820:1832	sulfation degree determination	1803:1832	sulfation degree determination	1803:1832	These limitations make it difficult to apply the current methods to quality control via sulfation degree determination.					
34213122	10	192	theme	various	1495:1501	arg1	methods					1514:1520	various analytical methods	1495:1520	various analytical methods based on chromatography or NMR	1495:1551	During the last two decades, various analytical methods based on chromatography or NMR have been developed for structural characterization of UFH and LMWHs.					
34213122	16	193	theme	electrophoresis	2306:2320	arg1	total					2337:2341	a total	2335:2341	a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated	2335:2524	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	16	193	theme	electrophoresis	2306:2320	arg1	method					2327:2332	a capillary electrophoresis (CE) method	2294:2332	a capillary electrophoresis (CE) method	2294:2332	The digestion of enoxaparin and heparin samples was performed at 25 ℃ for 48 h. By using a capillary electrophoresis (CE) method, a total of 18 oligosaccharides building blocks of heparin, including ten disaccharides, one trisaccharide, three tetrasaccharides, and four 1,6-anhydro derivatives, can be baseline separated.					
34213122	3	194	theme	bleeding	469:476	arg1	effects					539:545	less bleeding, greater bioavailability, and more predictable anticoagulant effects	464:545	less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin	464:570	LMWHs have largely replaced heparin as an anticoagulant for treatment and prevention of thrombosis because of the advantages of less bleeding, greater bioavailability, and more predictable anticoagulant effects in comparison to heparin.					
34213122	2	195	theme	molecular	214:222	arg1	LMWHs					241:245	LMWHs	241:245	LMWHs	241:245	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	2	195	theme	molecular	214:222	arg1	heparins					231:238	Low molecular weight heparins	210:238	Low molecular weight heparins (LMWHs)	210:246	Low molecular weight heparins (LMWHs) are derived from the unfractionated heparin (UFH) by enzymatic or chemical degradation.					
34213122	23	196	from	SD	3565:3566	arg1	manufacturers					3603:3615	the different manufacturers	3589:3615	the different manufacturers	3589:3615	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	14	197	theme	heparin	2007:2013	arg1	blocks					1997:2002	building blocks	1988:2002	building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases	1988:2091	The determination is based on the separation of building blocks of heparin obtained by exhaustive digestion of UFH and LMWHs in a mixture of heparinases.					
34213122	26	198	theme	satisfactory	4035:4046	arg1	data					4048:4051	The obtained satisfactory data	4022:4051	The obtained satisfactory data	4022:4051	The obtained satisfactory data show that high fluctuation in the sulfation degree of UFH could transmit to the final enoxaparin products.					
34213122	24	199	theme	enoxaparin	3808:3817	arg1	products					3819:3826	enoxaparin products	3808:3826	enoxaparin products from different manufacturers	3808:3855	The variation in terms of the sulfation degree of enoxaparin products from different manufacturers can be precisely identified using this method.					
34213122	23	200	theme	quality	3724:3730	arg1	control					3732:3738	quality control	3724:3738	quality control of LMWHs and UFH	3724:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	22	201	theme	total	3382:3386	arg1	T-SD					3406:3409	T-SD	3406:3409	T-SD	3406:3409	The total sulfation degree (T-SD), the individual degree of N-sulfation (N-SD) and O-sulfation (O-SD) data were also determined and compared.					
34213122	22	201	theme	total	3382:3386	arg1	degree					3398:3403	The total sulfation degree	3378:3403	The total sulfation degree (T-SD)	3378:3410	The total sulfation degree (T-SD), the individual degree of N-sulfation (N-SD) and O-sulfation (O-SD) data were also determined and compared.					
34213122	4	202	theme	alkaline	601:608	arg1	degradation					610:620	the alkaline degradation	597:620	the alkaline degradation of UFH	597:627	Enoxaparin, produced by the alkaline degradation of UFH through β-eliminative cleavage, represents the most commonly used LMWH.					
34213122	28	203	theme	compositional	4292:4304	arg1	analysis					4306:4313	quantitative compositional analysis	4279:4313	quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents	4279:4457	The CE method has several advantages for quantitative compositional analysis of LMWHs, such as high separation efficiency, high sensitivity, automation, short analysis time and low consumption of both sample and reagents.					
34213122	23	204	theme	methods	3712:3718	arg1	one					3665:3667	one	3665:3667	one	3665:3667	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	23	204	theme	methods	3712:3718	arg1	method					3643:3648	our method	3639:3648	our method	3639:3648	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	23	204	theme	methods	3712:3718	arg1	methods					3712:3718	the quantitative compositional analysis methods	3672:3718	the quantitative compositional analysis methods for quality control of LMWHs and UFH	3672:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	23	205	used	used	3657:3660	arg2	methods					3712:3718	the quantitative compositional analysis methods	3672:3718	the quantitative compositional analysis methods for quality control of LMWHs and UFH	3672:3755	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	23	205	used	used	3657:3660	arg2	method					3643:3648	our method	3639:3648	our method	3639:3648	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	23	205	used	used	3657:3660	arg2	one					3665:3667	one	3665:3667	one	3665:3667	A significant difference was observed in the SD of the products from the different manufacturers, which indicated that our method can be used as one of the quantitative compositional analysis methods for quality control of LMWHs and UFH.					
34213122	18	206	theme	accurate	2812:2819	arg1	number					2821:2826	the accurate number	2808:2826	the accurate number of sulfate ester groups in each disaccharide unit	2808:2876	Based on the assumption that the molar extinction coefficient of each oligosaccharide at UV 232 nm is the same, the concentration of each oligosaccharide can be conveniently replaced by their peak area, and the accurate number of sulfate ester groups in each disaccharide unit can be determined, hence the average sulfation degree (SD).					
34896139	4	0	theme	novel	472:476	arg1	development					478:488	a novel development	470:488	a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size	470:583	This research proposes a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size.					
34896139	10	1	theme	single-use	1511:1520	arg1	packaging					1522:1530	single-use packaging	1511:1530	single-use packaging	1511:1530	The mechanical and moisture properties of the composites also suggest their potential application in single-use packaging.					
34896139	9	2	theme	snippet	1366:1372	arg1	size					1374:1377	denim snippet size	1360:1377	denim snippet size	1360:1377	Overall, this work reveals an eco-friendly approach to utilise consumed denim waste, with insight on possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation.					
34896139	4	3	theme	snippet	572:578	arg1	size					580:583	different denim snippet size	556:583	different denim snippet size	556:583	This research proposes a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size.					
34896139	5	4	theme	higher	775:780	arg1	hygroscopicity					782:795	a higher hygroscopicity	773:795	a higher hygroscopicity	773:795	A smoother denim/cornstarch composite morphology and higher tensile strength were observed when smaller denim snippets were used, whereas larger snippets produced voids and roughness and a higher hygroscopicity.					
34896139	4	5	theme	denim/cornstarch	493:508	arg1	fractions					539:547	denim/cornstarch composite, maintaining 50/50 fractions	493:547	denim/cornstarch composite, maintaining 50/50 fractions	493:547	This research proposes a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size.					
34896139	8	6	dep	either	1160:1165	arg1	gelatinised					1167:1177	gelatinised	1167:1177	gelatinised	1167:1177	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
34896139	8	6	dep	either	1160:1165	arg1	used					1210:1213	used	1210:1213	used	1210:1213	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
34896139	7	7	theme	good	994:997	arg1	compatibility					1010:1022	a good structural compatibility	992:1022	a good structural compatibility	992:1022	The chemical structure was observed identical in all of the composites and a good structural compatibility perceived.					
34896139	9	8	theme	denim	1288:1292	arg1	waste					1294:1298	consumed denim waste	1279:1298	consumed denim waste	1279:1298	Overall, this work reveals an eco-friendly approach to utilise consumed denim waste, with insight on possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation.					
34896139	4	9	theme	denim	566:570	arg1	size					580:583	different denim snippet size	556:583	different denim snippet size	556:583	This research proposes a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size.					
34896139	4	10	dep	composite	510:518	arg1	maintaining					521:531	maintaining	521:531	maintaining	521:531	This research proposes a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size.					
34896139	10	11	theme	moisture	1429:1436	arg1	properties					1438:1447	The mechanical and moisture properties	1410:1447	properties	1438:1447	The mechanical and moisture properties of the composites also suggest their potential application in single-use packaging.					
34896139	5	12	theme	smoother	588:595	arg1	morphology					624:633	A smoother denim/cornstarch composite morphology	586:633	A smoother denim/cornstarch composite morphology	586:633	A smoother denim/cornstarch composite morphology and higher tensile strength were observed when smaller denim snippets were used, whereas larger snippets produced voids and roughness and a higher hygroscopicity.					
34896139	7	13	dep	composites	977:986	arg1	the					973:975	the	973:975	the	973:975	The chemical structure was observed identical in all of the composites and a good structural compatibility perceived.					
34896139	9	14	theme	eco-friendly	1246:1257	arg1	approach					1259:1266	an eco-friendly approach	1243:1266	an eco-friendly approach to utilise consumed denim waste	1243:1298	Overall, this work reveals an eco-friendly approach to utilise consumed denim waste, with insight on possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation.					
34896139	8	15	theme	cornstarch	1096:1105	arg1	peaks					1046:1050	Individual peaks	1035:1050	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1)	1035:1117	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
34896139	10	16	theme	potential	1486:1494	arg1	application					1496:1506	their potential application	1480:1506	their potential application in single-use packaging	1480:1530	The mechanical and moisture properties of the composites also suggest their potential application in single-use packaging.					
34896139	5	17	theme	denim/cornstarch	597:612	arg1	morphology					624:633	A smoother denim/cornstarch composite morphology	586:633	A smoother denim/cornstarch composite morphology	586:633	A smoother denim/cornstarch composite morphology and higher tensile strength were observed when smaller denim snippets were used, whereas larger snippets produced voids and roughness and a higher hygroscopicity.					
34896139	2	18	theme	little	268:273	arg1	attention					275:283	little attention	268:283	little attention	268:283	Though denim is highly rich in cellulose, its valorisation has received little attention.					
34896139	8	19	dep	denim	1060:1064	arg1	1705 cm-1					1081:1089	1705 cm-1	1081:1089	1705 cm-1	1081:1089	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
34896139	8	19	dep	denim	1060:1064	arg1	1105 cm-1					1067:1075	1105 cm-1	1067:1075	1105 cm-1	1067:1075	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
34896139	3	20	dep	few	288:290	arg1	attempts					292:299	attempts	292:299	attempts	292:299	A few attempts have previously shown prospects of denim in composites, though the ultimate products were non-compostable due to the nature of the chosen matrix.					
34896139	7	21	from	all	966:968	arg1	identical					953:961	identical	953:961	identical	953:961	The chemical structure was observed identical in all of the composites and a good structural compatibility perceived.					
34896139	5	22	theme	composite	614:622	arg1	morphology					624:633	A smoother denim/cornstarch composite morphology	586:633	A smoother denim/cornstarch composite morphology	586:633	A smoother denim/cornstarch composite morphology and higher tensile strength were observed when smaller denim snippets were used, whereas larger snippets produced voids and roughness and a higher hygroscopicity.					
34896139	3	23	theme	denim	336:340	arg1	prospects					323:331	prospects	323:331	prospects of denim in composites	323:354	A few attempts have previously shown prospects of denim in composites, though the ultimate products were non-compostable due to the nature of the chosen matrix.					
34896139	9	24	theme	denim	1360:1364	arg1	size					1374:1377	denim snippet size	1360:1377	denim snippet size	1360:1377	Overall, this work reveals an eco-friendly approach to utilise consumed denim waste, with insight on possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation.					
34896139	0	25	theme	Sustainable	0:10	arg1	composites					38:47	Sustainable biodegradable denim waste composites	0:47	Sustainable biodegradable denim waste composites for potential single-use packaging	0:82	Sustainable biodegradable denim waste composites for potential single-use packaging.					
34896139	0	26	theme	biodegradable	12:24	arg1	composites					38:47	Sustainable biodegradable denim waste composites	0:47	Sustainable biodegradable denim waste composites for potential single-use packaging	0:82	Sustainable biodegradable denim waste composites for potential single-use packaging.					
34896139	4	27	dep	fractions	539:547	arg1	using					550:554	using	550:554	using different denim snippet size	550:583	This research proposes a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size.					
34896139	5	28	theme	higher	639:644	arg1	strength					654:661	higher tensile strength	639:661	higher tensile strength	639:661	A smoother denim/cornstarch composite morphology and higher tensile strength were observed when smaller denim snippets were used, whereas larger snippets produced voids and roughness and a higher hygroscopicity.					
34896139	10	29	from	application	1496:1506	arg1	packaging					1522:1530	single-use packaging	1511:1530	single-use packaging	1511:1530	The mechanical and moisture properties of the composites also suggest their potential application in single-use packaging.					
34896139	2	30	from	rich	219:222	arg1	cellulose					227:235	cellulose	227:235	cellulose	227:235	Though denim is highly rich in cellulose, its valorisation has received little attention.					
34896139	3	31	theme	ultimate	368:375	arg1	non-compostable					391:405	non-compostable	391:405	non-compostable	391:405	A few attempts have previously shown prospects of denim in composites, though the ultimate products were non-compostable due to the nature of the chosen matrix.					
34896139	3	31	theme	ultimate	368:375	arg1	products					377:384	the ultimate products	364:384	the ultimate products	364:384	A few attempts have previously shown prospects of denim in composites, though the ultimate products were non-compostable due to the nature of the chosen matrix.					
34896139	9	32	theme	size	1374:1377	arg1	alteration					1346:1355	alteration	1346:1355	alteration of denim snippet size and cornstarch gelatinisation	1346:1407	Overall, this work reveals an eco-friendly approach to utilise consumed denim waste, with insight on possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation.					
34896139	5	33	theme	tensile	646:652	arg1	strength					654:661	higher tensile strength	639:661	higher tensile strength	639:661	A smoother denim/cornstarch composite morphology and higher tensile strength were observed when smaller denim snippets were used, whereas larger snippets produced voids and roughness and a higher hygroscopicity.					
34896139	8	34	theme	denim	1060:1064	arg1	peaks					1046:1050	Individual peaks	1035:1050	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1)	1035:1117	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
34896139	1	35	theme	massive	98:104	arg1	tons					126:129	a massive 2.16 million metric tons	96:129	a massive 2.16 million metric tons of denim jeans	96:144	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	1	35	theme	massive	98:104	arg1	wasted					158:163	wasted	158:163	wasted	158:163	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	0	36	theme	waste	32:36	arg1	composites					38:47	Sustainable biodegradable denim waste composites	0:47	Sustainable biodegradable denim waste composites for potential single-use packaging	0:82	Sustainable biodegradable denim waste composites for potential single-use packaging.					
34896139	4	37	theme	fractions	539:547	arg1	development					478:488	a novel development	470:488	a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size	470:583	This research proposes a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size.					
34896139	5	38	used	used	710:713	arg2	snippets					696:703	smaller denim snippets	682:703	smaller denim snippets	682:703	A smoother denim/cornstarch composite morphology and higher tensile strength were observed when smaller denim snippets were used, whereas larger snippets produced voids and roughness and a higher hygroscopicity.					
34896139	10	39	theme	composites	1456:1465	arg1	properties					1438:1447	The mechanical and moisture properties	1410:1447	properties	1438:1447	The mechanical and moisture properties of the composites also suggest their potential application in single-use packaging.					
34896139	1	40	theme	2.16	106:109	arg1	tons					126:129	a massive 2.16 million metric tons	96:129	a massive 2.16 million metric tons of denim jeans	96:144	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	1	40	theme	2.16	106:109	arg1	wasted					158:163	wasted	158:163	wasted	158:163	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	2	41	from	cellulose	227:235	arg1	rich					219:222	rich	219:222	rich	219:222	Though denim is highly rich in cellulose, its valorisation has received little attention.					
34896139	3	42	theme	matrix	439:444	arg1	nature					418:423	the nature	414:423	the nature of the chosen matrix	414:444	A few attempts have previously shown prospects of denim in composites, though the ultimate products were non-compostable due to the nature of the chosen matrix.					
34896139	0	43	theme	denim	26:30	arg1	composites					38:47	Sustainable biodegradable denim waste composites	0:47	Sustainable biodegradable denim waste composites for potential single-use packaging	0:82	Sustainable biodegradable denim waste composites for potential single-use packaging.					
34896139	4	44	theme	different	556:564	arg1	size					580:583	different denim snippet size	556:583	different denim snippet size	556:583	This research proposes a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size.					
34896139	1	45	theme	million	111:117	arg1	tons					126:129	a massive 2.16 million metric tons	96:129	a massive 2.16 million metric tons of denim jeans	96:144	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	1	45	theme	million	111:117	arg1	wasted					158:163	wasted	158:163	wasted	158:163	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	6	46	theme	semi-gelatinised	822:837	arg1	cornstarch					839:848	semi-gelatinised cornstarch	822:848	semi-gelatinised cornstarch	822:848	Composite prepared from semi-gelatinised cornstarch showed a completely flexible structure similar to non-woven sheet.					
34896139	8	47	dep	confirmed	1124:1132	arg1	cornstarch					1199:1208	semi-gelatinised cornstarch	1182:1208	semi-gelatinised cornstarch	1182:1208	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
34896139	8	47	dep	confirmed	1124:1132	arg1	either					1160:1165	either	1160:1165	either	1160:1165	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
34896139	9	48	theme	gelatinisation	1394:1407	arg1	alteration					1346:1355	alteration	1346:1355	alteration of denim snippet size and cornstarch gelatinisation	1346:1407	Overall, this work reveals an eco-friendly approach to utilise consumed denim waste, with insight on possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation.					
34896139	6	49	theme	non-woven	900:908	arg1	sheet					910:914	non-woven sheet	900:914	non-woven sheet	900:914	Composite prepared from semi-gelatinised cornstarch showed a completely flexible structure similar to non-woven sheet.					
34896139	9	50	theme	cornstarch	1383:1392	arg1	gelatinisation					1394:1407	cornstarch gelatinisation	1383:1407	cornstarch gelatinisation	1383:1407	Overall, this work reveals an eco-friendly approach to utilise consumed denim waste, with insight on possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation.					
34896139	8	51	theme	Individual	1035:1044	arg1	peaks					1046:1050	Individual peaks	1035:1050	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1)	1035:1117	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
34896139	5	52	theme	larger	724:729	arg1	snippets					731:738	larger snippets	724:738	larger snippets	724:738	A smoother denim/cornstarch composite morphology and higher tensile strength were observed when smaller denim snippets were used, whereas larger snippets produced voids and roughness and a higher hygroscopicity.					
34896139	9	53	theme	consumed	1279:1286	arg1	waste					1294:1298	consumed denim waste	1279:1298	consumed denim waste	1279:1298	Overall, this work reveals an eco-friendly approach to utilise consumed denim waste, with insight on possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation.					
34896139	3	54	theme	chosen	432:437	arg1	matrix					439:444	the chosen matrix	428:444	the chosen matrix	428:444	A few attempts have previously shown prospects of denim in composites, though the ultimate products were non-compostable due to the nature of the chosen matrix.					
34896139	0	55	theme	single-use	63:72	arg1	packaging					74:82	potential single-use packaging	53:82	potential single-use packaging	53:82	Sustainable biodegradable denim waste composites for potential single-use packaging.					
34896139	4	56	theme	composite	510:518	arg1	fractions					539:547	denim/cornstarch composite, maintaining 50/50 fractions	493:547	denim/cornstarch composite, maintaining 50/50 fractions	493:547	This research proposes a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size.					
34896139	5	57	theme	smaller	682:688	arg1	snippets					696:703	smaller denim snippets	682:703	smaller denim snippets	682:703	A smoother denim/cornstarch composite morphology and higher tensile strength were observed when smaller denim snippets were used, whereas larger snippets produced voids and roughness and a higher hygroscopicity.					
34896139	6	58	theme	similar	889:895	arg1	structure					879:887	a completely flexible structure	857:887	a completely flexible structure similar to non-woven sheet	857:914	Composite prepared from semi-gelatinised cornstarch showed a completely flexible structure similar to non-woven sheet.					
34896139	1	59	theme	metric	119:124	arg1	tons					126:129	a massive 2.16 million metric tons	96:129	a massive 2.16 million metric tons of denim jeans	96:144	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	1	59	theme	metric	119:124	arg1	wasted					158:163	wasted	158:163	wasted	158:163	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	0	60	theme	potential	53:61	arg1	packaging					74:82	potential single-use packaging	53:82	potential single-use packaging	53:82	Sustainable biodegradable denim waste composites for potential single-use packaging.					
34896139	4	61	theme	50/50	533:537	arg1	fractions					539:547	denim/cornstarch composite, maintaining 50/50 fractions	493:547	denim/cornstarch composite, maintaining 50/50 fractions	493:547	This research proposes a novel development of denim/cornstarch composite, maintaining 50/50 fractions, using different denim snippet size.					
34896139	5	62	theme	denim	690:694	arg1	snippets					696:703	smaller denim snippets	682:703	smaller denim snippets	682:703	A smoother denim/cornstarch composite morphology and higher tensile strength were observed when smaller denim snippets were used, whereas larger snippets produced voids and roughness and a higher hygroscopicity.					
34896139	9	63	theme	possible	1317:1324	arg1	fine-tuning					1326:1336	possible fine-tuning	1317:1336	possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation	1317:1407	Overall, this work reveals an eco-friendly approach to utilise consumed denim waste, with insight on possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation.					
34896139	1	64	dep	year	91:94	arg1	goes					176:179	goes	176:179	mostly goes into landfill	169:193	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	1	64	dep	year	91:94	arg1	tons					126:129	a massive 2.16 million metric tons	96:129	a massive 2.16 million metric tons of denim jeans	96:144	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	1	64	dep	year	91:94	arg1	wasted					158:163	wasted	158:163	wasted	158:163	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	6	65	theme	flexible	870:877	arg1	structure					879:887	a completely flexible structure	857:887	a completely flexible structure similar to non-woven sheet	857:914	Composite prepared from semi-gelatinised cornstarch showed a completely flexible structure similar to non-woven sheet.					
34896139	7	66	theme	structural	999:1008	arg1	compatibility					1010:1022	a good structural compatibility	992:1022	a good structural compatibility	992:1022	The chemical structure was observed identical in all of the composites and a good structural compatibility perceived.					
34896139	7	67	from	identical	953:961	arg1	all					966:968	all	966:968	all	966:968	The chemical structure was observed identical in all of the composites and a good structural compatibility perceived.					
34896139	7	67	from	identical	953:961	arg1	composites					977:986	composites	977:986	composites	977:986	The chemical structure was observed identical in all of the composites and a good structural compatibility perceived.					
34896139	7	67	from	identical	953:961	arg1	compatibility					1010:1022	a good structural compatibility	992:1022	a good structural compatibility	992:1022	The chemical structure was observed identical in all of the composites and a good structural compatibility perceived.					
34896139	8	68	theme	composite	1141:1149	arg1	samples					1151:1157	all composite samples	1137:1157	all composite samples	1137:1157	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
34896139	1	69	theme	denim	134:138	arg1	jeans					140:144	denim jeans	134:144	denim jeans	134:144	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	3	70	from	prospects	323:331	arg1	composites					345:354	composites	345:354	composites	345:354	A few attempts have previously shown prospects of denim in composites, though the ultimate products were non-compostable due to the nature of the chosen matrix.					
34896139	10	71	theme	mechanical	1414:1423	arg1	properties					1438:1447	The mechanical and moisture properties	1410:1447	properties	1438:1447	The mechanical and moisture properties of the composites also suggest their potential application in single-use packaging.					
34896139	1	72	theme	jeans	140:144	arg1	tons					126:129	a massive 2.16 million metric tons	96:129	a massive 2.16 million metric tons of denim jeans	96:144	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	1	72	theme	jeans	140:144	arg1	wasted					158:163	wasted	158:163	wasted	158:163	Every year a massive 2.16 million metric tons of denim jeans is globally wasted and mostly goes into landfill.					
34896139	9	73	from	insight	1306:1312	arg1	fine-tuning					1326:1336	possible fine-tuning	1317:1336	possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation	1317:1407	Overall, this work reveals an eco-friendly approach to utilise consumed denim waste, with insight on possible fine-tuning through alteration of denim snippet size and cornstarch gelatinisation.					
34896139	7	74	theme	chemical	921:928	arg1	structure					930:938	The chemical structure	917:938	The chemical structure	917:938	The chemical structure was observed identical in all of the composites and a good structural compatibility perceived.					
34896139	8	75	theme	semi-gelatinised	1182:1197	arg1	cornstarch					1199:1208	semi-gelatinised cornstarch	1182:1208	semi-gelatinised cornstarch	1182:1208	Individual peaks of both denim (1105 cm-1 and 1705 cm-1) and cornstarch (1081 cm-1) were confirmed in all composite samples, either gelatinised or semi-gelatinised cornstarch used.					
33939578	4	0	from	effects	1170:1176	arg1	activities					1220:1229	in vitro antioxidant activities	1199:1229	in vitro antioxidant activities	1199:1229	Moreover, PLPS exhibited more evident scavenging effects on free radicals and in vitro antioxidant activities than C-PLPS.					
33939578	4	0	from	effects	1170:1176	arg1	radicals					1186:1193	free radicals	1181:1193	free radicals	1181:1193	Moreover, PLPS exhibited more evident scavenging effects on free radicals and in vitro antioxidant activities than C-PLPS.					
33939578	3	1	theme	±	971:971	arg1	content					955:961	higher carbohydrate content	935:961	higher carbohydrate content (63.58% ± 1.12%)	935:978	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	1	theme	±	971:971	arg1	%					977:977	63.58% ± 1.12%	964:977	63.58% ± 1.12%	964:977	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	2	theme	precipitation	887:899	arg1	protocols					915:923	traditional ethanol precipitation and isolation protocols	867:923	traditional ethanol precipitation and isolation protocols	867:923	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	4	3	theme	antioxidant	1208:1218	arg1	activities					1220:1229	in vitro antioxidant activities	1199:1229	in vitro antioxidant activities	1199:1229	Moreover, PLPS exhibited more evident scavenging effects on free radicals and in vitro antioxidant activities than C-PLPS.					
33939578	0	4	theme	two-phase	75:83	arg1	system					85:90	an aqueous two-phase system	64:90	an aqueous two-phase system	64:90	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	5	5	theme	effective	1313:1321	arg1	strategy					1323:1330	an effective strategy	1310:1330	an effective strategy for the separation/purification of highly bioactive polysaccharides	1310:1398	Therefore, the method of [Chol]Cl/K2HPO4 ATPS can be developed as an effective strategy for the separation/purification of highly bioactive polysaccharides.					
33939578	5	5	theme	effective	1313:1321	arg1	method					1259:1264	the method	1255:1264	the method of [Chol]Cl/K2HPO4 ATPS	1255:1288	Therefore, the method of [Chol]Cl/K2HPO4 ATPS can be developed as an effective strategy for the separation/purification of highly bioactive polysaccharides.					
33939578	3	6	theme	%	969:969	arg1	content					955:961	higher carbohydrate content	935:961	higher carbohydrate content (63.58% ± 1.12%)	935:978	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	6	theme	%	969:969	arg1	%					977:977	63.58% ± 1.12%	964:977	63.58% ± 1.12%	964:977	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	2	7	theme	distilled	737:745	arg1	4.0 mL					754:759	4.0 mL	754:759	4.0 mL	754:759	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	7	theme	distilled	737:745	arg1	water					747:751	distilled water	737:751	distilled water (4.0 mL)	737:760	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	5	8	theme	polysaccharides	1384:1398	arg1	separation/purification					1340:1362	the separation/purification	1336:1362	the separation/purification of highly bioactive polysaccharides	1336:1398	Therefore, the method of [Chol]Cl/K2HPO4 ATPS can be developed as an effective strategy for the separation/purification of highly bioactive polysaccharides.					
33939578	0	9	from	Purification	0:11	arg1	linteus					47:53	Phellinus linteus	37:53	Phellinus linteus	37:53	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	2	10	theme	%	573:573	arg1	PLPS					575:578	0.29% PLPS	569:578	68.53% ± 0.29% PLPS	560:578	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	1	11	theme	physicochemical	406:420	arg1	properties					438:447	the physicochemical and antioxidant properties	402:447	the physicochemical and antioxidant properties of PLPS after ATPS extraction	402:477	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	5	12	theme	[Chol	1269:1273	arg1	ATPS					1285:1288	[Chol]Cl/K2HPO4 ATPS	1269:1288	[Chol]Cl/K2HPO4 ATPS	1269:1288	Therefore, the method of [Chol]Cl/K2HPO4 ATPS can be developed as an effective strategy for the separation/purification of highly bioactive polysaccharides.					
33939578	3	13	theme	similar	1071:1077	arg1	characterizations					1102:1118	similar preliminary structural characterizations	1071:1118	similar preliminary structural characterizations	1071:1118	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	14	theme	traditional	867:877	arg1	protocols					915:923	traditional ethanol precipitation and isolation protocols	867:923	traditional ethanol precipitation and isolation protocols	867:923	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	2	15	theme	0.29	569:572	arg1	%					573:573	%	573:573	%	573:573	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	16	theme	proteins	599:606	arg1	efficiencies					544:555	the maximal extraction efficiencies	521:555	the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins	521:606	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	17	theme	10.0 mg	690:696	arg1	1.0 mL					724:729	1.0 mL	724:729	1.0 mL	724:729	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	17	theme	10.0 mg	690:696	arg1	extract					715:721	10.0 mg mL-1 crude water extract	690:721	10.0 mg mL-1 crude water extract (1.0 mL)	690:730	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	4	18	theme	in vitro	1199:1206	arg1	activities					1220:1229	in vitro antioxidant activities	1199:1229	in vitro antioxidant activities	1199:1229	Moreover, PLPS exhibited more evident scavenging effects on free radicals and in vitro antioxidant activities than C-PLPS.					
33939578	2	19	theme	extraction	533:542	arg1	efficiencies					544:555	the maximal extraction efficiencies	521:555	the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins	521:606	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	1	20	theme	antioxidant	426:436	arg1	properties					438:447	the physicochemical and antioxidant properties	402:447	the physicochemical and antioxidant properties of PLPS after ATPS extraction	402:477	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	3	21	theme	monosaccharide	1031:1044	arg1	compositions					1046:1057	different monosaccharide compositions	1021:1057	different monosaccharide compositions	1021:1057	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	21	theme	monosaccharide	1031:1044	arg1	content					955:961	higher carbohydrate content	935:961	higher carbohydrate content (63.58% ± 1.12%)	935:978	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	22	theme	isolation	905:913	arg1	protocols					915:923	traditional ethanol precipitation and isolation protocols	867:923	traditional ethanol precipitation and isolation protocols	867:923	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	23	theme	different	1021:1029	arg1	compositions					1046:1057	different monosaccharide compositions	1021:1057	different monosaccharide compositions	1021:1057	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	23	theme	different	1021:1029	arg1	content					955:961	higher carbohydrate content	935:961	higher carbohydrate content (63.58% ± 1.12%)	935:978	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	0	24	from	linteus	47:53	arg1	polysaccharides					16:30	polysaccharides	16:30	polysaccharides from Phellinus linteus	16:53	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	0	24	from	linteus	47:53	arg1	Purification					0:11	Purification	0:11	Purification of polysaccharides from Phellinus linteus	0:53	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	2	25	theme	±	591:591	arg1	%					597:597	82.37% ± 0.41%	584:597	82.37% ± 0.41%	584:597	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	3	26	theme	carbohydrate	942:953	arg1	compositions					1046:1057	different monosaccharide compositions	1021:1057	different monosaccharide compositions	1021:1057	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	26	theme	carbohydrate	942:953	arg1	content					955:961	higher carbohydrate content	935:961	higher carbohydrate content (63.58% ± 1.12%)	935:978	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	26	theme	carbohydrate	942:953	arg1	weight					997:1002	lower molecular weight	981:1002	lower molecular weight (15.2 kDa, 80%)	981:1018	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	26	theme	carbohydrate	942:953	arg1	%					977:977	63.58% ± 1.12%	964:977	63.58% ± 1.12%	964:977	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	1	27	theme	chloride	370:377	arg1	/K2HPO4					389:395	choline chloride ([Chol]Cl)/K2HPO4	362:395	choline chloride ([Chol]Cl)/K2HPO4	362:395	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	2	28	theme	%	589:589	arg1	%					597:597	82.37% ± 0.41%	584:597	82.37% ± 0.41%	584:597	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	29	contain	contained	652:660	arg2	water					747:751	distilled water	737:751	distilled water (4.0 mL)	737:760	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	29	contain	contained	652:660	arg2	K2HPO4					668:673	68.9% K2HPO4	662:673	68.9% K2HPO4	662:673	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	29	contain	contained	652:660	arg2	Cl					686:687	20% [Chol]Cl	676:687	20% [Chol]Cl	676:687	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	29	contain	contained	652:660	arg2	ATPS					647:650	the cholinium-based ATPS	627:650	the cholinium-based ATPS	627:650	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	29	contain	contained	652:660	arg2	1.0 mL					724:729	1.0 mL	724:729	1.0 mL	724:729	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	29	contain	contained	652:660	arg1	time					773:776	time	773:776	time	773:776	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	29	contain	contained	652:660	arg2	extract					715:721	10.0 mg mL-1 crude water extract	690:721	10.0 mg mL-1 crude water extract (1.0 mL)	690:730	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	29	contain	contained	652:660	arg2	4.0 mL					754:759	4.0 mL	754:759	4.0 mL	754:759	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	29	contain	contained	652:660	arg1	temperature					782:792	temperature	782:792	temperature	782:792	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	29	contain	contained	652:660	arg1	ATPS					647:650	the cholinium-based ATPS	627:650	the cholinium-based ATPS	627:650	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	30	theme	maximal	525:531	arg1	efficiencies					544:555	the maximal extraction efficiencies	521:555	the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins	521:606	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	1	31	theme	P.	290:291	arg1	mycelia					301:307	P. linteus mycelia	290:307	P. linteus mycelia	290:307	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	5	32	theme	bioactive	1374:1382	arg1	polysaccharides					1384:1398	highly bioactive polysaccharides	1367:1398	highly bioactive polysaccharides	1367:1398	Therefore, the method of [Chol]Cl/K2HPO4 ATPS can be developed as an effective strategy for the separation/purification of highly bioactive polysaccharides.					
33939578	1	33	theme	PLPS	452:455	arg1	properties					438:447	the physicochemical and antioxidant properties	402:447	the physicochemical and antioxidant properties of PLPS after ATPS extraction	402:477	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	2	34	theme	%	666:666	arg1	K2HPO4					668:673	68.9% K2HPO4	662:673	68.9% K2HPO4	662:673	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	3	35	theme	preliminary	1079:1089	arg1	characterizations					1102:1118	similar preliminary structural characterizations	1071:1118	similar preliminary structural characterizations	1071:1118	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	1	36	theme	linteus	293:299	arg1	mycelia					301:307	P. linteus mycelia	290:307	P. linteus mycelia	290:307	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	3	37	theme	molecular	987:995	arg1	content					955:961	higher carbohydrate content	935:961	higher carbohydrate content (63.58% ± 1.12%)	935:978	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	37	theme	molecular	987:995	arg1	weight					997:1002	lower molecular weight	981:1002	lower molecular weight (15.2 kDa, 80%)	981:1018	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	2	38	theme	%	597:597	arg1	proteins					599:606	68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins	560:606	68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins	560:606	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	39	theme	water	709:713	arg1	1.0 mL					724:729	1.0 mL	724:729	1.0 mL	724:729	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	39	theme	water	709:713	arg1	extract					715:721	10.0 mg mL-1 crude water extract	690:721	10.0 mg mL-1 crude water extract (1.0 mL)	690:730	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	1	40	theme	Phellinus	201:209	arg1	PLPS					236:239	PLPS	236:239	PLPS	236:239	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	1	40	theme	Phellinus	201:209	arg1	polysaccharides					219:233	Phellinus linteus polysaccharides	201:233	Phellinus linteus polysaccharides (PLPS)	201:240	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	0	41	theme	polysaccharides	16:30	arg1	Purification					0:11	Purification	0:11	Purification of polysaccharides from Phellinus linteus	0:53	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	2	42	dep	%	565:565	arg1	PLPS					575:578	0.29% PLPS	569:578	68.53% ± 0.29% PLPS	560:578	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	0	43	theme	physicochemical	114:128	arg1	properties					146:155	the physicochemical and antioxidant properties	110:155	the physicochemical and antioxidant properties of polysaccharides in vitro	110:183	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	4	44	theme	free	1181:1184	arg1	radicals					1186:1193	free radicals	1181:1193	free radicals	1181:1193	Moreover, PLPS exhibited more evident scavenging effects on free radicals and in vitro antioxidant activities than C-PLPS.					
33939578	2	45	theme	crude	703:707	arg1	1.0 mL					724:729	1.0 mL	724:729	1.0 mL	724:729	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	45	theme	crude	703:707	arg1	extract					715:721	10.0 mg mL-1 crude water extract	690:721	10.0 mg mL-1 crude water extract (1.0 mL)	690:730	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	1	46	theme	linteus	211:217	arg1	PLPS					236:239	PLPS	236:239	PLPS	236:239	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	1	46	theme	linteus	211:217	arg1	polysaccharides					219:233	Phellinus linteus polysaccharides	201:233	Phellinus linteus polysaccharides (PLPS)	201:240	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	2	47	theme	30 min	797:802	arg1	temperature					782:792	temperature	782:792	temperature	782:792	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	47	theme	30 min	797:802	arg1	time					773:776	time	773:776	time	773:776	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	0	48	theme	properties	146:155	arg1	evaluation					96:105	evaluation	96:105	evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro	96:183	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	0	48	theme	properties	146:155	arg1	system					85:90	an aqueous two-phase system	64:90	an aqueous two-phase system	64:90	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	5	49	theme	Cl/K2HPO4	1275:1283	arg1	ATPS					1285:1288	[Chol]Cl/K2HPO4 ATPS	1269:1288	[Chol]Cl/K2HPO4 ATPS	1269:1288	Therefore, the method of [Chol]Cl/K2HPO4 ATPS can be developed as an effective strategy for the separation/purification of highly bioactive polysaccharides.					
33939578	2	50	theme	mL-1	698:701	arg1	1.0 mL					724:729	1.0 mL	724:729	1.0 mL	724:729	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	50	theme	mL-1	698:701	arg1	extract					715:721	10.0 mg mL-1 crude water extract	690:721	10.0 mg mL-1 crude water extract (1.0 mL)	690:730	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	0	51	theme	Phellinus	37:45	arg1	linteus					47:53	Phellinus linteus	37:53	Phellinus linteus	37:53	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	0	52	theme	antioxidant	134:144	arg1	properties					146:155	the physicochemical and antioxidant properties	110:155	the physicochemical and antioxidant properties of polysaccharides in vitro	110:183	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	1	53	theme	ATPS	463:466	arg1	extraction					468:477	ATPS extraction	463:477	ATPS extraction	463:477	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	3	54	contain	had	931:933	arg1	PLPS					926:929	PLPS	926:929	PLPS	926:929	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	54	contain	had	931:933	arg2	content					955:961	higher carbohydrate content	935:961	higher carbohydrate content (63.58% ± 1.12%)	935:978	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	54	contain	had	931:933	arg2	compositions					1046:1057	different monosaccharide compositions	1021:1057	different monosaccharide compositions	1021:1057	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	54	contain	had	931:933	arg2	%					977:977	63.58% ± 1.12%	964:977	63.58% ± 1.12%	964:977	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	54	contain	had	931:933	arg2	weight					997:1002	lower molecular weight	981:1002	lower molecular weight (15.2 kDa, 80%)	981:1018	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	1	55	dep	chloride	370:377	arg1	[Chol					380:384	[Chol	380:384	[Chol	380:384	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	0	56	theme	polysaccharides	160:174	arg1	in vitro					176:183	polysaccharides in vitro	160:183	polysaccharides in vitro	160:183	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	2	57	theme	21.2 °C	808:814	arg1	temperature					782:792	temperature	782:792	temperature	782:792	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	57	theme	21.2 °C	808:814	arg1	time					773:776	time	773:776	time	773:776	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	1	58	theme	choline	362:368	arg1	chloride					370:377	choline chloride	362:377	choline chloride ([Chol]Cl)/K2HPO4	362:395	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	1	59	theme	aqueous	321:327	arg1	ATPS					347:350	ATPS	347:350	ATPS	347:350	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	1	59	theme	aqueous	321:327	arg1	system					339:344	an aqueous two-phase system	318:344	an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4	318:395	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	2	60	theme	%	565:565	arg1	proteins					599:606	68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins	560:606	68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins	560:606	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	4	61	theme	evident	1151:1157	arg1	effects					1170:1176	more evident scavenging effects	1146:1176	more evident scavenging effects on free radicals and in vitro antioxidant activities	1146:1229	Moreover, PLPS exhibited more evident scavenging effects on free radicals and in vitro antioxidant activities than C-PLPS.					
33939578	3	62	theme	lower	981:985	arg1	content					955:961	higher carbohydrate content	935:961	higher carbohydrate content (63.58% ± 1.12%)	935:978	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	62	theme	lower	981:985	arg1	weight					997:1002	lower molecular weight	981:1002	lower molecular weight (15.2 kDa, 80%)	981:1018	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	2	63	theme	68.9	662:665	arg1	%					666:666	%	666:666	%	666:666	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	1	64	theme	two-phase	329:337	arg1	ATPS					347:350	ATPS	347:350	ATPS	347:350	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	1	64	theme	two-phase	329:337	arg1	system					339:344	an aqueous two-phase system	318:344	an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4	318:395	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
33939578	5	65	theme	ATPS	1285:1288	arg1	strategy					1323:1330	an effective strategy	1310:1330	an effective strategy for the separation/purification of highly bioactive polysaccharides	1310:1398	Therefore, the method of [Chol]Cl/K2HPO4 ATPS can be developed as an effective strategy for the separation/purification of highly bioactive polysaccharides.					
33939578	5	65	theme	ATPS	1285:1288	arg1	method					1259:1264	the method	1255:1264	the method of [Chol]Cl/K2HPO4 ATPS	1255:1288	Therefore, the method of [Chol]Cl/K2HPO4 ATPS can be developed as an effective strategy for the separation/purification of highly bioactive polysaccharides.					
33939578	3	66	theme	ethanol	879:885	arg1	precipitation					887:899	ethanol precipitation	879:899	ethanol precipitation	879:899	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	2	67	theme	shaking	765:771	arg1	time					773:776	time	773:776	time	773:776	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	0	68	theme	in vitro	176:183	arg1	properties					146:155	the physicochemical and antioxidant properties	110:155	the physicochemical and antioxidant properties of polysaccharides in vitro	110:183	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	2	69	theme	[Chol	680:684	arg1	Cl					686:687	20% [Chol]Cl	676:687	20% [Chol]Cl	676:687	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	2	70	theme	cholinium-based	631:645	arg1	ATPS					647:650	the cholinium-based ATPS	627:650	the cholinium-based ATPS	627:650	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	0	71	theme	aqueous	67:73	arg1	system					85:90	an aqueous two-phase system	64:90	an aqueous two-phase system	64:90	Purification of polysaccharides from Phellinus linteus by using an aqueous two-phase system and evaluation of the physicochemical and antioxidant properties of polysaccharides in vitro.					
33939578	4	72	theme	scavenging	1159:1168	arg1	effects					1170:1176	more evident scavenging effects	1146:1176	more evident scavenging effects on free radicals and in vitro antioxidant activities	1146:1229	Moreover, PLPS exhibited more evident scavenging effects on free radicals and in vitro antioxidant activities than C-PLPS.					
33939578	2	73	theme	%	678:678	arg1	Cl					686:687	20% [Chol]Cl	676:687	20% [Chol]Cl	676:687	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	3	74	theme	higher	935:940	arg1	compositions					1046:1057	different monosaccharide compositions	1021:1057	different monosaccharide compositions	1021:1057	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	74	theme	higher	935:940	arg1	content					955:961	higher carbohydrate content	935:961	higher carbohydrate content (63.58% ± 1.12%)	935:978	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	74	theme	higher	935:940	arg1	weight					997:1002	lower molecular weight	981:1002	lower molecular weight (15.2 kDa, 80%)	981:1018	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	74	theme	higher	935:940	arg1	%					977:977	63.58% ± 1.12%	964:977	63.58% ± 1.12%	964:977	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	75	dep	weight	997:1002	arg1	15.2 kDa					1005:1012	15.2 kDa	1005:1012	15.2 kDa	1005:1012	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	75	dep	weight	997:1002	arg1	%					1017:1017	80%	1015:1017	80%	1015:1017	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	3	76	theme	structural	1091:1100	arg1	characterizations					1102:1118	similar preliminary structural characterizations	1071:1118	similar preliminary structural characterizations	1071:1118	Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations.					
33939578	2	77	theme	20	676:677	arg1	%					678:678	%	678:678	%	678:678	Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively.					
33939578	1	78	dep	[Chol	380:384	arg1	Cl					386:387	Cl	386:387	Cl	386:387	In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated.					
34127221	5	0	theme	metalloproteinase	970:986	arg1	expression					996:1005	matrix metalloproteinase (MMP)-9 expression	963:1005	matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process	963:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	1	theme	underlying	842:851	arg1	attributable					921:932	attributable	921:932	attributable	921:932	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	1	theme	underlying	842:851	arg1	mechanism					853:861	The underlying mechanism	838:861	The underlying mechanism of the composite beneficial to wound remodeling	838:909	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	0	2	theme	wound	110:114	arg1	healing					116:122	wound healing	110:122	wound healing	110:122	Fabrication and characterization of Rhizochitosan and its incorporation with platelet concentrates to promote wound healing.					
34127221	2	3	theme	sporangium-free	325:339	arg1	mattress					350:357	sporangium-free mycelial mattress	325:357	sporangium-free mycelial mattress produced from Rhizopus stolonifer F6	325:394	Rhizochitosan was fabricated by deacetylation of Rhizochitin, which obtained by simply depigmenting sporangium-free mycelial mattress produced from Rhizopus stolonifer F6.					
34127221	6	4	theme	injury	1202:1207	arg1	wound					1209:1213	full-thickness injury wound	1187:1213	full-thickness injury wound	1187:1213	Conclusively, the composite dressing demonstrated to be highly beneficial to the healing of full-thickness injury wound.					
34127221	3	5	theme	Rhizochitosan	434:446	arg1	characterizations					413:429	Physicochemical characterizations	397:429	Physicochemical characterizations of Rhizochitosan	397:446	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	5	6	theme	higher	1028:1033	arg1	level					1052:1056	a higher MMP-2 expression level	1026:1056	a higher MMP-2 expression level in a later stage of healing process	1026:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	4	7	theme	functional	796:805	arg1	tissue					807:812	functional tissue	796:812	functional tissue instead of scar tissue	796:835	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	3	8	theme	hydrogel	563:570	arg1	nature					572:577	exhibiting hydrogel nature	552:577	exhibiting hydrogel nature after hydration	552:593	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	3	9	theme	Physicochemical	397:411	arg1	characterizations					413:429	Physicochemical characterizations	397:429	Physicochemical characterizations of Rhizochitosan	397:446	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	3	10	theme	nature	572:577	arg1	ability					518:524	a water-absorption ability	499:524	a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration	499:593	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	5	11	theme	MMP-2	1035:1039	arg1	level					1052:1056	a higher MMP-2 expression level	1026:1056	a higher MMP-2 expression level in a later stage of healing process	1026:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	12	theme	-9	993:994	arg1	expression					996:1005	matrix metalloproteinase (MMP)-9 expression	963:1005	matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process	963:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	4	13	theme	individual	731:740	arg1	components					742:751	each individual components	726:751	each individual components	726:751	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	4	14	theme	composite	664:672	arg1	dressing					674:681	the composite dressing	660:681	the composite dressing	660:681	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	4	15	with	rats	627:630	arg1	injury					652:657	full-thickness injury	637:657	full-thickness injury	637:657	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	5	16	from	level	1052:1056	arg1	stage					1069:1073	a later stage	1061:1073	a later stage of healing process	1061:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	16	from	level	1052:1056	arg1	level					954:958	a more reduction level	937:958	a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process	937:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	1	17	theme	wound	196:200	arg1	healing					202:208	wound healing	196:208	wound healing	196:208	Composite dressing composed of Rhizochitosan and Regenplex™ to promote wound healing were assessed.					
34127221	5	18	from	expression	996:1005	arg1	stage					1016:1020	early stage	1010:1020	early stage	1010:1020	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	18	from	expression	996:1005	arg1	level					1052:1056	a higher MMP-2 expression level	1026:1056	a higher MMP-2 expression level in a later stage of healing process	1026:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	19	theme	reduction	944:952	arg1	level					954:958	a more reduction level	937:958	a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process	937:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	3	20	contain	contained	469:477	arg1	it					466:467	it	466:467	it	466:467	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	3	20	contain	contained	469:477	arg2	chitosan					485:492	13.5% chitosan	479:492	13.5% chitosan	479:492	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	5	21	theme	more	939:942	arg1	level					954:958	a more reduction level	937:958	a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process	937:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	3	22	theme	water-absorption	501:516	arg1	ability					518:524	a water-absorption ability	499:524	a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration	499:593	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	5	23	theme	wound	894:898	arg1	remodeling					900:909	wound remodeling	894:909	wound remodeling	894:909	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	24	theme	expression	996:1005	arg1	level					954:958	a more reduction level	937:958	a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process	937:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	4	25	theme	scar	825:828	arg1	tissue					830:835	scar tissue	825:835	functional tissue instead of scar tissue	796:835	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	3	26	theme	weight	541:546	arg1	ability					518:524	a water-absorption ability	499:524	a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration	499:593	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	5	27	theme	early	1010:1014	arg1	stage					1016:1020	early stage	1010:1020	early stage	1010:1020	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	2	28	theme	mycelial	341:348	arg1	mattress					350:357	sporangium-free mycelial mattress	325:357	sporangium-free mycelial mattress produced from Rhizopus stolonifer F6	325:394	Rhizochitosan was fabricated by deacetylation of Rhizochitin, which obtained by simply depigmenting sporangium-free mycelial mattress produced from Rhizopus stolonifer F6.					
34127221	4	29	theme	full-thickness	637:650	arg1	injury					652:657	full-thickness injury	637:657	full-thickness injury	637:657	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	4	30	from	study	615:619	arg1	rats					627:630	SD rats	624:630	SD rats with full-thickness injury	624:657	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	6	31	theme	composite	1113:1121	arg1	beneficial					1158:1167	beneficial	1158:1167	beneficial	1158:1167	Conclusively, the composite dressing demonstrated to be highly beneficial to the healing of full-thickness injury wound.					
34127221	6	31	theme	composite	1113:1121	arg1	dressing					1123:1130	the composite dressing	1109:1130	the composite dressing	1109:1130	Conclusively, the composite dressing demonstrated to be highly beneficial to the healing of full-thickness injury wound.					
34127221	3	32	theme	35-fold	529:535	arg1	weight					541:546	35-fold dry weight	529:546	35-fold dry weight	529:546	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	0	33	theme	Rhizochitosan	36:48	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of Rhizochitosan and its incorporation with platelet concentrates to promote wound healing.					
34127221	0	33	theme	Rhizochitosan	36:48	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of Rhizochitosan and its incorporation with platelet concentrates to promote wound healing.					
34127221	3	34	theme	dry	537:539	arg1	weight					541:546	35-fold dry weight	529:546	35-fold dry weight	529:546	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	3	35	theme	13.5	479:482	arg1	%					483:483	%	483:483	%	483:483	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	4	36	theme	wound-healing	601:613	arg1	study					615:619	a wound-healing study	599:619	a wound-healing study on SD rats with full-thickness injury	599:657	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	5	37	theme	composite	870:878	arg1	attributable					921:932	attributable	921:932	attributable	921:932	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	37	theme	composite	870:878	arg1	mechanism					853:861	The underlying mechanism	838:861	The underlying mechanism of the composite beneficial to wound remodeling	838:909	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	38	theme	healing	1078:1084	arg1	process					1086:1092	healing process	1078:1092	healing process	1078:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	0	39	with	characterization	16:31	arg1	platelet					77:84	platelet	77:84	platelet	77:84	Fabrication and characterization of Rhizochitosan and its incorporation with platelet concentrates to promote wound healing.					
34127221	1	40	theme	Composite	125:133	arg1	dressing					135:142	Composite dressing	125:142	Composite dressing composed of Rhizochitosan and Regenplex™ to promote wound healing	125:208	Composite dressing composed of Rhizochitosan and Regenplex™ to promote wound healing were assessed.					
34127221	3	41	theme	%	483:483	arg1	chitosan					485:492	13.5% chitosan	479:492	13.5% chitosan	479:492	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	4	42	theme	SD	624:625	arg1	rats					627:630	SD rats	624:630	SD rats with full-thickness injury	624:657	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	5	43	theme	process	1086:1092	arg1	stage					1069:1073	a later stage	1061:1073	a later stage of healing process	1061:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	44	from	stage	1016:1020	arg1	stage					1069:1073	a later stage	1061:1073	a later stage of healing process	1061:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	44	from	stage	1016:1020	arg1	level					954:958	a more reduction level	937:958	a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process	937:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	45	theme	later	1063:1067	arg1	stage					1069:1073	a later stage	1061:1073	a later stage of healing process	1061:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	0	46	with	Fabrication	0:10	arg1	platelet					77:84	platelet	77:84	platelet	77:84	Fabrication and characterization of Rhizochitosan and its incorporation with platelet concentrates to promote wound healing.					
34127221	2	47	theme	stolonifer	382:391	arg1	F6					393:394	Rhizopus stolonifer F6	373:394	Rhizopus stolonifer F6	373:394	Rhizochitosan was fabricated by deacetylation of Rhizochitin, which obtained by simply depigmenting sporangium-free mycelial mattress produced from Rhizopus stolonifer F6.					
34127221	6	48	theme	wound	1209:1213	arg1	healing					1176:1182	the healing	1172:1182	the healing of full-thickness injury wound	1172:1213	Conclusively, the composite dressing demonstrated to be highly beneficial to the healing of full-thickness injury wound.					
34127221	5	49	theme	expression	1041:1050	arg1	level					1052:1056	a higher MMP-2 expression level	1026:1056	a higher MMP-2 expression level in a later stage of healing process	1026:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	2	50	theme	Rhizopus	373:380	arg1	F6					393:394	Rhizopus stolonifer F6	373:394	Rhizopus stolonifer F6	373:394	Rhizochitosan was fabricated by deacetylation of Rhizochitin, which obtained by simply depigmenting sporangium-free mycelial mattress produced from Rhizopus stolonifer F6.					
34127221	6	51	theme	full-thickness	1187:1200	arg1	wound					1209:1213	full-thickness injury wound	1187:1213	full-thickness injury wound	1187:1213	Conclusively, the composite dressing demonstrated to be highly beneficial to the healing of full-thickness injury wound.					
34127221	3	52	theme	exhibiting	552:561	arg1	nature					572:577	exhibiting hydrogel nature	552:577	exhibiting hydrogel nature after hydration	552:593	Physicochemical characterizations of Rhizochitosan demonstrated that it contained 13.5% chitosan with a water-absorption ability of 35-fold dry weight and exhibiting hydrogel nature after hydration.					
34127221	4	53	contain	had	683:685	arg2	effect					704:709	a better healing effect	687:709	a better healing effect	687:709	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	4	53	contain	had	683:685	arg1	dressing					674:681	the composite dressing	660:681	the composite dressing	660:681	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	5	54	from	level	954:958	arg1	stage					1016:1020	early stage	1010:1020	early stage	1010:1020	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	54	from	level	954:958	arg1	level					1052:1056	a higher MMP-2 expression level	1026:1056	a higher MMP-2 expression level in a later stage of healing process	1026:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	55	theme	matrix	963:968	arg1	metalloproteinase					970:986	matrix metalloproteinase	963:986	matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process	963:1092	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	5	55	theme	matrix	963:968	arg1	MMP					989:991	MMP	989:991	MMP	989:991	The underlying mechanism of the composite beneficial to wound remodeling is likely attributable to a more reduction level of matrix metalloproteinase (MMP)-9 expression in early stage and a higher MMP-2 expression level in a later stage of healing process.					
34127221	4	56	theme	each	726:729	arg1	components					742:751	each individual components	726:751	each individual components	726:751	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	4	57	theme	healing	696:702	arg1	effect					704:709	a better healing effect	687:709	a better healing effect	687:709	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	0	58	theme	incorporation	58:70	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of Rhizochitosan and its incorporation with platelet concentrates to promote wound healing.					
34127221	0	58	theme	incorporation	58:70	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of Rhizochitosan and its incorporation with platelet concentrates to promote wound healing.					
34127221	2	59	theme	Rhizochitin	274:284	arg1	deacetylation					257:269	deacetylation	257:269	deacetylation of Rhizochitin, which obtained by simply depigmenting sporangium-free mycelial mattress produced from Rhizopus stolonifer F6	257:394	Rhizochitosan was fabricated by deacetylation of Rhizochitin, which obtained by simply depigmenting sporangium-free mycelial mattress produced from Rhizopus stolonifer F6.					
34127221	4	60	theme	control	757:763	arg1	group					765:769	control group	757:769	control group	757:769	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34127221	4	61	theme	better	689:694	arg1	effect					704:709	a better healing effect	687:709	a better healing effect	687:709	In a wound-healing study on SD rats with full-thickness injury, the composite dressing had a better healing effect than those for each individual components and control group and wound even healed as functional tissue instead of scar tissue.					
34480673	8	0	theme	chromatography-porous	975:995	arg1	UPLC-PGC-FTMS/MS					1053:1068	UPLC-PGC-FTMS/MS	1053:1068	UPLC-PGC-FTMS/MS	1053:1068	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	0	theme	chromatography-porous	975:995	arg1	spectrometry					1039:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry	945:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS)	945:1069	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	2	1	theme	tract	379:383	arg1	health					348:353	the health	344:353	the health of the gastrointestinal tract in pigs	344:391	The composition of intestinal mucin O-glycans can affect the health of the gastrointestinal tract in pigs.					
34480673	7	2	theme	heparin	753:759	arg1	extraction					761:770	heparin extraction	753:770	heparin extraction	753:770	After heparin extraction, mucin was digested with trypsin, and separated by strong anion exchange chromatography.					
34480673	12	3	theme	immunological	1477:1489	arg1	results					1500:1506	the immunological activity results	1473:1506	the immunological activity results	1473:1506	Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators.					
34480673	8	4	theme	carbon-Fourier	1009:1022	arg1	UPLC-PGC-FTMS/MS					1053:1068	UPLC-PGC-FTMS/MS	1053:1068	UPLC-PGC-FTMS/MS	1053:1068	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	4	theme	carbon-Fourier	1009:1022	arg1	spectrometry					1039:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry	945:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS)	945:1069	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	2	5	theme	intestinal	306:315	arg1	O-glycans					323:331	intestinal mucin O-glycans	306:331	intestinal mucin O-glycans	306:331	The composition of intestinal mucin O-glycans can affect the health of the gastrointestinal tract in pigs.					
34480673	8	6	theme	mass	1034:1037	arg1	UPLC-PGC-FTMS/MS					1053:1068	UPLC-PGC-FTMS/MS	1053:1068	UPLC-PGC-FTMS/MS	1053:1068	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	6	theme	mass	1034:1037	arg1	spectrometry					1039:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry	945:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS)	945:1069	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	10	7	theme	total	1250:1254	arg1	O-glycans					1256:1264	the total O-glycans	1246:1264	the total O-glycans	1246:1264	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	10	7	theme	total	1250:1254	arg1	different					1278:1286	different	1278:1286	different	1278:1286	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	5	8	from	structure	555:563	arg1	mucosa					614:619	porcine intestinal mucosa	595:619	porcine intestinal mucosa	595:619	The structure of mucin derived O-glycans in porcine intestinal mucosa are currently unknown.					
34480673	11	9	gly	sialylated	1395:1404	arg1	content					1387:1393	The high content	1378:1393	The high content sialylated mucin	1378:1410	The high content sialylated mucin may explain its effect in biological processes.					
34480673	0	10	theme	activity	136:143	arg1	evaluation					145:154	immunological activity evaluation	122:154	immunological activity evaluation	122:154	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	2	11	theme	O-glycans	323:331	arg1	composition					291:301	The composition	287:301	The composition of intestinal mucin O-glycans	287:331	The composition of intestinal mucin O-glycans can affect the health of the gastrointestinal tract in pigs.					
34480673	7	12	theme	anion	830:834	arg1	chromatography					845:858	strong anion exchange chromatography	823:858	strong anion exchange chromatography	823:858	After heparin extraction, mucin was digested with trypsin, and separated by strong anion exchange chromatography.					
34480673	6	13	theme	extraction	699:708	arg1	residues					710:717	the heparin extraction residues	687:717	the heparin extraction residues	687:717	In this study, we isolated the mucins from the heparin extraction residues and profiled the O-glycans.					
34480673	11	14	theme	high	1382:1385	arg1	content					1387:1393	The high content	1378:1393	The high content sialylated mucin	1378:1410	The high content sialylated mucin may explain its effect in biological processes.					
34480673	5	15	link	derived	574:580	arg1	O-glycans					582:590	mucin derived O-glycans	568:590	mucin derived O-glycans	568:590	The structure of mucin derived O-glycans in porcine intestinal mucosa are currently unknown.					
34480673	11	16	from	effect	1428:1433	arg1	processes					1449:1457	biological processes	1438:1457	biological processes	1438:1457	The high content sialylated mucin may explain its effect in biological processes.					
34480673	5	17	theme	derived	574:580	arg1	O-glycans					582:590	mucin derived O-glycans	568:590	mucin derived O-glycans	568:590	The structure of mucin derived O-glycans in porcine intestinal mucosa are currently unknown.					
34480673	2	18	from	health	348:353	arg1	pigs					388:391	pigs	388:391	pigs	388:391	The composition of intestinal mucin O-glycans can affect the health of the gastrointestinal tract in pigs.					
34480673	6	19	theme	heparin	691:697	arg1	residues					710:717	the heparin extraction residues	687:717	the heparin extraction residues	687:717	In this study, we isolated the mucins from the heparin extraction residues and profiled the O-glycans.					
34480673	10	20	theme	O-glycans	1256:1264	arg1	O-glycans					1256:1264	the total O-glycans	1246:1264	the total O-glycans	1246:1264	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	10	20	theme	O-glycans	1256:1264	arg1	different					1278:1286	different	1278:1286	different	1278:1286	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	10	20	theme	O-glycans	1256:1264	arg1	%					1241:1241	71.93%	1236:1241	71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon	1236:1375	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	8	21	theme	mucin	865:869	arg1	O-glycans					879:887	The mucin derived O-glycans	861:887	The mucin derived O-glycans	861:887	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	12	22	theme	cytotoxic	1609:1617	arg1	effects					1619:1625	any cytotoxic effects	1605:1625	any cytotoxic effects	1605:1625	Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators.					
34480673	9	23	link	3-derived	1144:1152	arg1	glycans					1154:1160	Core 3-derived glycans	1139:1160	Core 3-derived glycans	1139:1160	Thirty five kinds of O-glycans were identified, most of which were Core 3-derived glycans.					
34480673	0	24	theme	O-glycan	99:106	arg1	profiling					108:116	O-glycan profiling	99:116	O-glycan profiling	99:116	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	4	25	theme	heparin	487:493	arg1	residues					506:513	The heparin extraction residues	483:513	The heparin extraction residues rich in mucins	483:528	The heparin extraction residues rich in mucins were usually wasted.					
34480673	4	25	theme	heparin	487:493	arg1	wasted					543:548	wasted	543:548	wasted	543:548	The heparin extraction residues rich in mucins were usually wasted.					
34480673	8	26	theme	alkaline	905:912	arg1	elimination					916:926	alkaline β elimination	905:926	alkaline β elimination	905:926	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	10	27	theme	other	1301:1305	arg1	intestine					1332:1340	mouse intestine	1326:1340	mouse intestine	1326:1340	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	10	27	theme	other	1301:1305	arg1	intestine					1348:1356	fish intestine	1343:1356	fish intestine	1343:1356	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	10	27	theme	other	1301:1305	arg1	species					1307:1313	other species	1301:1313	other species	1301:1313	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	10	27	theme	other	1301:1305	arg1	colon					1371:1375	porcine colon	1363:1375	porcine colon	1363:1375	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	3	28	theme	main	442:445	arg1	material					451:458	the main raw material	438:458	the main raw material of heparin extraction	438:480	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	3	28	theme	main	442:445	arg1	mucosa					413:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	5	29	theme	intestinal	603:612	arg1	mucosa					614:619	porcine intestinal mucosa	595:619	porcine intestinal mucosa	595:619	The structure of mucin derived O-glycans in porcine intestinal mucosa are currently unknown.					
34480673	3	30	theme	Porcine	394:400	arg1	mucosa					413:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	3	30	theme	Porcine	394:400	arg1	material					451:458	the main raw material	438:458	the main raw material of heparin extraction	438:480	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	9	31	theme	3-derived	1144:1152	arg1	glycans					1154:1160	Core 3-derived glycans	1139:1160	Core 3-derived glycans	1139:1160	Thirty five kinds of O-glycans were identified, most of which were Core 3-derived glycans.					
34480673	0	32	theme	mucin-type	18:27	arg1	glycopeptide					29:40	Highly sialylated mucin-type glycopeptide	0:40	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.	0:155	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	12	33	theme	intestinal	1535:1544	arg1	mucin					1546:1550	the porcine intestinal mucin	1523:1550	the porcine intestinal mucin	1523:1550	Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators.					
34480673	10	34	theme	mouse	1326:1330	arg1	intestine					1332:1340	mouse intestine	1326:1340	mouse intestine	1326:1340	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	3	35	theme	heparin	463:469	arg1	extraction					471:480	heparin extraction	463:480	heparin extraction	463:480	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	10	36	theme	acid	1210:1213	arg1	Neu5Ac					1215:1220	sialic acid Neu5Ac	1203:1220	sialic acid Neu5Ac	1203:1220	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	0	37	gly	sialylated	7:16	arg1	glycopeptide					29:40	Highly sialylated mucin-type glycopeptide	0:40	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.	0:155	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	2	38	theme	gastrointestinal	362:377	arg1	tract					379:383	the gastrointestinal tract	358:383	the gastrointestinal tract	358:383	The composition of intestinal mucin O-glycans can affect the health of the gastrointestinal tract in pigs.					
34480673	0	39	theme	intestinal	55:64	arg1	mucosa					66:71	porcine intestinal mucosa	47:71	porcine intestinal mucosa	47:71	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	8	40	link	derived	871:877	arg1	O-glycans					879:887	The mucin derived O-glycans	861:887	The mucin derived O-glycans	861:887	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	41	theme	high	951:954	arg1	UPLC-PGC-FTMS/MS					1053:1068	UPLC-PGC-FTMS/MS	1053:1068	UPLC-PGC-FTMS/MS	1053:1068	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	41	theme	high	951:954	arg1	spectrometry					1039:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry	945:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS)	945:1069	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	9	42	theme	Core	1139:1142	arg1	glycans					1154:1160	Core 3-derived glycans	1139:1160	Core 3-derived glycans	1139:1160	Thirty five kinds of O-glycans were identified, most of which were Core 3-derived glycans.					
34480673	8	43	theme	liquid	968:973	arg1	UPLC-PGC-FTMS/MS					1053:1068	UPLC-PGC-FTMS/MS	1053:1068	UPLC-PGC-FTMS/MS	1053:1068	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	43	theme	liquid	968:973	arg1	spectrometry					1039:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry	945:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS)	945:1069	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	10	44	contain	containing	1192:1201	arg2	Neu5Ac					1215:1220	sialic acid Neu5Ac	1203:1220	sialic acid Neu5Ac	1203:1220	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	10	44	contain	containing	1192:1201	arg1	O-glycans					1182:1190	the O-glycans	1178:1190	the O-glycans containing sialic acid Neu5Ac	1178:1220	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	8	45	dep	spectrometry	1039:1050	arg1	transform					1024:1032	transform	1024:1032	transform	1024:1032	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	46	theme	β	914:914	arg1	elimination					916:926	alkaline β elimination	905:926	alkaline β elimination	905:926	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	47	theme	graphitized	997:1007	arg1	UPLC-PGC-FTMS/MS					1053:1068	UPLC-PGC-FTMS/MS	1053:1068	UPLC-PGC-FTMS/MS	1053:1068	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	47	theme	graphitized	997:1007	arg1	spectrometry					1039:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry	945:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS)	945:1069	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	1	48	theme	pathogens	266:274	arg1	infection					276:284	pathogens infection	266:284	pathogens infection	266:284	Mucins are the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection.					
34480673	12	49	theme	activity	1491:1498	arg1	results					1500:1506	the immunological activity results	1473:1506	the immunological activity results	1473:1506	Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators.					
34480673	4	50	from	mucins	523:528	arg1	rich					515:518	rich	515:518	rich	515:518	The heparin extraction residues rich in mucins were usually wasted.					
34480673	2	51	theme	mucin	317:321	arg1	O-glycans					323:331	intestinal mucin O-glycans	306:331	intestinal mucin O-glycans	306:331	The composition of intestinal mucin O-glycans can affect the health of the gastrointestinal tract in pigs.					
34480673	6	52	from	residues	710:717	arg1	mucins					675:680	the mucins	671:680	the mucins from the heparin extraction residues	671:717	In this study, we isolated the mucins from the heparin extraction residues and profiled the O-glycans.					
34480673	5	53	dep	derived	574:580	arg1	mucin					568:572	mucin	568:572	mucin	568:572	The structure of mucin derived O-glycans in porcine intestinal mucosa are currently unknown.					
34480673	4	54	from	rich	515:518	arg1	mucins					523:528	mucins	523:528	mucins	523:528	The heparin extraction residues rich in mucins were usually wasted.					
34480673	0	55	gly	glycopeptide	29:40	arg2	glycopeptide					29:40	Highly sialylated mucin-type glycopeptide	0:40	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.	0:155	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	0	55	gly	glycopeptide	29:40	arg1	mucosa					66:71	porcine intestinal mucosa	47:71	porcine intestinal mucosa	47:71	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	1	56	theme	intestinal	211:220	arg1	layer					229:233	the intestinal mucosa layer	207:233	the intestinal mucosa layer	207:233	Mucins are the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection.					
34480673	10	57	from	that	1293:1296	arg1	O-glycans					1256:1264	the total O-glycans	1246:1264	the total O-glycans	1246:1264	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	10	57	from	that	1293:1296	arg1	different					1278:1286	different	1278:1286	different	1278:1286	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	7	58	theme	exchange	836:843	arg1	chromatography					845:858	strong anion exchange chromatography	823:858	strong anion exchange chromatography	823:858	After heparin extraction, mucin was digested with trypsin, and separated by strong anion exchange chromatography.					
34480673	5	59	theme	O-glycans	582:590	arg1	structure					555:563	The structure	551:563	The structure of mucin derived O-glycans in porcine intestinal mucosa	551:619	The structure of mucin derived O-glycans in porcine intestinal mucosa are currently unknown.					
34480673	5	59	theme	O-glycans	582:590	arg1	unknown					635:641	unknown	635:641	unknown	635:641	The structure of mucin derived O-glycans in porcine intestinal mucosa are currently unknown.					
34480673	0	60	theme	heparin	79:85	arg1	extraction					87:96	heparin extraction	79:96	heparin extraction	79:96	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	9	61	theme	O-glycans	1093:1101	arg1	kinds					1084:1088	Thirty five kinds	1072:1088	Thirty five kinds of O-glycans	1072:1101	Thirty five kinds of O-glycans were identified, most of which were Core 3-derived glycans.					
34480673	1	62	theme	major	172:176	arg1	Mucins					157:162	Mucins	157:162	Mucins	157:162	Mucins are the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection.					
34480673	1	62	theme	major	172:176	arg1	proteins					178:185	the major proteins	168:185	the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection	168:284	Mucins are the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection.					
34480673	0	63	from	mucosa	66:71	arg1	glycopeptide					29:40	Highly sialylated mucin-type glycopeptide	0:40	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.	0:155	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	8	64	theme	derived	871:877	arg1	O-glycans					879:887	The mucin derived O-glycans	861:887	The mucin derived O-glycans	861:887	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	4	65	theme	extraction	495:504	arg1	residues					506:513	The heparin extraction residues	483:513	The heparin extraction residues rich in mucins	483:528	The heparin extraction residues rich in mucins were usually wasted.					
34480673	4	65	theme	extraction	495:504	arg1	wasted					543:548	wasted	543:548	wasted	543:548	The heparin extraction residues rich in mucins were usually wasted.					
34480673	0	66	theme	immunological	122:134	arg1	evaluation					145:154	immunological activity evaluation	122:154	immunological activity evaluation	122:154	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	1	67	theme	mucosa	222:227	arg1	layer					229:233	the intestinal mucosa layer	207:233	the intestinal mucosa layer	207:233	Mucins are the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection.					
34480673	4	68	theme	rich	515:518	arg1	residues					506:513	The heparin extraction residues	483:513	The heparin extraction residues rich in mucins	483:528	The heparin extraction residues rich in mucins were usually wasted.					
34480673	4	68	theme	rich	515:518	arg1	wasted					543:548	wasted	543:548	wasted	543:548	The heparin extraction residues rich in mucins were usually wasted.					
34480673	11	69	theme	biological	1438:1447	arg1	processes					1449:1457	biological processes	1438:1457	biological processes	1438:1457	The high content sialylated mucin may explain its effect in biological processes.					
34480673	0	70	theme	sialylated	7:16	arg1	glycopeptide					29:40	Highly sialylated mucin-type glycopeptide	0:40	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.	0:155	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	5	71	theme	porcine	595:601	arg1	mucosa					614:619	porcine intestinal mucosa	595:619	porcine intestinal mucosa	595:619	The structure of mucin derived O-glycans in porcine intestinal mucosa are currently unknown.					
34480673	10	72	theme	fish	1343:1346	arg1	intestine					1348:1356	fish intestine	1343:1356	fish intestine	1343:1356	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	3	73	theme	raw	447:449	arg1	material					451:458	the main raw material	438:458	the main raw material of heparin extraction	438:480	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	3	73	theme	raw	447:449	arg1	mucosa					413:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	7	74	theme	strong	823:828	arg1	chromatography					845:858	strong anion exchange chromatography	823:858	strong anion exchange chromatography	823:858	After heparin extraction, mucin was digested with trypsin, and separated by strong anion exchange chromatography.					
34480673	12	75	theme	immunomodulators	1664:1679	arg1	development					1649:1659	the development	1645:1659	the development of immunomodulators	1645:1679	Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators.					
34480673	3	76	theme	intestinal	402:411	arg1	mucosa					413:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	3	76	theme	intestinal	402:411	arg1	material					451:458	the main raw material	438:458	the main raw material of heparin extraction	438:480	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	0	77	theme	porcine	47:53	arg1	mucosa					66:71	porcine intestinal mucosa	47:71	porcine intestinal mucosa	47:71	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	12	78	theme	porcine	1527:1533	arg1	mucin					1546:1550	the porcine intestinal mucin	1523:1550	the porcine intestinal mucin	1523:1550	Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators.					
34480673	10	79	theme	porcine	1363:1369	arg1	colon					1371:1375	porcine colon	1363:1375	porcine colon	1363:1375	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	3	80	theme	extraction	471:480	arg1	material					451:458	the main raw material	438:458	the main raw material of heparin extraction	438:480	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	3	80	theme	extraction	471:480	arg1	mucosa					413:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	10	81	theme	sialic	1203:1208	arg1	acid					1210:1213	sialic acid	1203:1213	sialic acid Neu5Ac	1203:1220	In particular, the O-glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O-glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon.					
34480673	3	82	used	used	430:433	arg2	material					451:458	the main raw material	438:458	the main raw material of heparin extraction	438:480	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	3	82	used	used	430:433	arg2	mucosa					413:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa	394:418	Porcine intestinal mucosa is widely used as the main raw material of heparin extraction.					
34480673	0	83	dep	glycopeptide	29:40	arg1	profiling					108:116	O-glycan profiling	99:116	O-glycan profiling	99:116	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	0	83	dep	glycopeptide	29:40	arg1	evaluation					145:154	immunological activity evaluation	122:154	immunological activity evaluation	122:154	Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation.					
34480673	8	84	theme	ultra	945:949	arg1	UPLC-PGC-FTMS/MS					1053:1068	UPLC-PGC-FTMS/MS	1053:1068	UPLC-PGC-FTMS/MS	1053:1068	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	84	theme	ultra	945:949	arg1	spectrometry					1039:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry	945:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS)	945:1069	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	85	theme	performance	956:966	arg1	UPLC-PGC-FTMS/MS					1053:1068	UPLC-PGC-FTMS/MS	1053:1068	UPLC-PGC-FTMS/MS	1053:1068	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
34480673	8	85	theme	performance	956:966	arg1	spectrometry					1039:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry	945:1050	ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS)	945:1069	The mucin derived O-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS).					
33259188	7	0	theme	aerogel	1203:1209	arg1	products					1211:1218	the end aerogel products	1195:1218	the end aerogel products	1195:1218	Zein incorporation induced remarkable changes in the mechanical performance of the end aerogel products and showed a preventive effect on the morphological changes during the storage period.					
33259188	2	1	theme	regenerative	365:376	arg1	medicine					378:385	regenerative medicine	365:385	regenerative medicine	365:385	However, the lack of macropores in conventionally manufactured polysaccharide aerogels is a limitation to their use as scaffolds for regenerative medicine.					
33259188	6	2	theme	Harmonization	970:982	arg1	guideline					984:992	Harmonization guideline	970:992	Harmonization guideline of climatic conditions	970:1015	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	5	3	theme	composition	764:774	arg1	terms					755:759	terms	755:759	terms of composition as well as morphological, textural, and mechanical properties	755:836	The obtained dual-porous aerogels were evaluated in terms of composition as well as morphological, textural, and mechanical properties.					
33259188	6	4	theme	morphological	1055:1067	arg1	perspectives					1102:1113	morphological, physicochemical, and mechanical perspectives	1055:1113	morphological, physicochemical, and mechanical perspectives	1055:1113	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	5	5	theme	dual-porous	716:726	arg1	aerogels					728:735	The obtained dual-porous aerogels	703:735	The obtained dual-porous aerogels	703:735	The obtained dual-porous aerogels were evaluated in terms of composition as well as morphological, textural, and mechanical properties.					
33259188	7	6	theme	end	1199:1201	arg1	products					1211:1218	the end aerogel products	1195:1218	the end aerogel products	1195:1218	Zein incorporation induced remarkable changes in the mechanical performance of the end aerogel products and showed a preventive effect on the morphological changes during the storage period.					
33259188	2	7	theme	polysaccharide	295:308	arg1	aerogels					310:317	conventionally manufactured polysaccharide aerogels	267:317	conventionally manufactured polysaccharide aerogels	267:317	However, the lack of macropores in conventionally manufactured polysaccharide aerogels is a limitation to their use as scaffolds for regenerative medicine.					
33259188	6	8	theme	International	944:956	arg1	Council					958:964	the International Council	940:964	the International Council for Harmonization guideline of climatic conditions	940:1015	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	5	9	theme	properties	827:836	arg1	terms					755:759	terms	755:759	terms of composition as well as morphological, textural, and mechanical properties	755:836	The obtained dual-porous aerogels were evaluated in terms of composition as well as morphological, textural, and mechanical properties.					
33259188	4	10	theme	aerogel	617:623	arg1	backbone					625:632	the starch aerogel backbone	606:632	the starch aerogel backbone	606:632	In this work, the induction of a new macropore population (1-2 μm) well integrated into the starch aerogel backbone was successfully achieved by the incorporation of zein as a porogen.					
33259188	1	11	theme	Starch	78:83	arg1	aerogels					85:92	Starch aerogels	78:92	Starch aerogels	78:92	Starch aerogels are attractive materials for biomedical applications because of their low density and high open porosity coupled with high surface areas.					
33259188	1	11	theme	Starch	78:83	arg1	materials					109:117	attractive materials	98:117	attractive materials for biomedical applications	98:145	Starch aerogels are attractive materials for biomedical applications because of their low density and high open porosity coupled with high surface areas.					
33259188	1	12	theme	high	180:183	arg1	porosity					190:197	high open porosity	180:197	high open porosity	180:197	Starch aerogels are attractive materials for biomedical applications because of their low density and high open porosity coupled with high surface areas.					
33259188	6	13	dep	°C	900:901	arg1	humidity					917:924	65% relative humidity	904:924	65% relative humidity	904:924	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	5	14	theme	textural	802:809	arg1	properties					827:836	morphological, textural, and mechanical properties	787:836	composition as well as morphological, textural, and mechanical properties	764:836	The obtained dual-porous aerogels were evaluated in terms of composition as well as morphological, textural, and mechanical properties.					
33259188	1	15	theme	open	185:188	arg1	porosity					190:197	high open porosity	180:197	high open porosity	180:197	Starch aerogels are attractive materials for biomedical applications because of their low density and high open porosity coupled with high surface areas.					
33259188	7	16	theme	products	1211:1218	arg1	performance					1180:1190	the mechanical performance	1165:1190	the mechanical performance of the end aerogel products	1165:1218	Zein incorporation induced remarkable changes in the mechanical performance of the end aerogel products and showed a preventive effect on the morphological changes during the storage period.					
33259188	7	17	theme	morphological	1258:1270	arg1	changes					1272:1278	the morphological changes	1254:1278	the morphological changes during the storage period	1254:1304	Zein incorporation induced remarkable changes in the mechanical performance of the end aerogel products and showed a preventive effect on the morphological changes during the storage period.					
33259188	3	18	theme	aerogels	444:451	arg1	storage					418:424	storage	418:424	storage of polysaccharide aerogels	418:451	Moreover, the stability under storage of polysaccharide aerogels is critical for biomedical purposes and scarcely studied so far.					
33259188	0	19	theme	Stability	0:8	arg1	Studies					10:16	Stability Studies	0:16	Stability Studies of Starch	0:26	Stability Studies of Starch Aerogel Formulations for Biomedical Applications.					
33259188	4	20	theme	macropore	555:563	arg1	population					565:574	a new macropore population	549:574	a new macropore population (1-2 μm) well integrated into the starch aerogel backbone	549:632	In this work, the induction of a new macropore population (1-2 μm) well integrated into the starch aerogel backbone was successfully achieved by the incorporation of zein as a porogen.					
33259188	4	20	theme	macropore	555:563	arg1	μm					581:582	1-2 μm	577:582	1-2 μm	577:582	In this work, the induction of a new macropore population (1-2 μm) well integrated into the starch aerogel backbone was successfully achieved by the incorporation of zein as a porogen.					
33259188	1	21	theme	attractive	98:107	arg1	aerogels					85:92	Starch aerogels	78:92	Starch aerogels	78:92	Starch aerogels are attractive materials for biomedical applications because of their low density and high open porosity coupled with high surface areas.					
33259188	1	21	theme	attractive	98:107	arg1	materials					109:117	attractive materials	98:117	attractive materials for biomedical applications	98:145	Starch aerogels are attractive materials for biomedical applications because of their low density and high open porosity coupled with high surface areas.					
33259188	0	22	theme	Starch	21:26	arg1	Studies					10:16	Stability Studies	0:16	Stability Studies of Starch	0:26	Stability Studies of Starch Aerogel Formulations for Biomedical Applications.					
33259188	4	23	theme	new	551:553	arg1	population					565:574	a new macropore population	549:574	a new macropore population (1-2 μm) well integrated into the starch aerogel backbone	549:632	In this work, the induction of a new macropore population (1-2 μm) well integrated into the starch aerogel backbone was successfully achieved by the incorporation of zein as a porogen.					
33259188	4	23	theme	new	551:553	arg1	μm					581:582	1-2 μm	577:582	1-2 μm	577:582	In this work, the induction of a new macropore population (1-2 μm) well integrated into the starch aerogel backbone was successfully achieved by the incorporation of zein as a porogen.					
33259188	2	24	from	lack	245:248	arg1	aerogels					310:317	conventionally manufactured polysaccharide aerogels	267:317	conventionally manufactured polysaccharide aerogels	267:317	However, the lack of macropores in conventionally manufactured polysaccharide aerogels is a limitation to their use as scaffolds for regenerative medicine.					
33259188	5	25	theme	mechanical	816:825	arg1	properties					827:836	morphological, textural, and mechanical properties	787:836	composition as well as morphological, textural, and mechanical properties	764:836	The obtained dual-porous aerogels were evaluated in terms of composition as well as morphological, textural, and mechanical properties.					
33259188	2	26	theme	manufactured	282:293	arg1	aerogels					310:317	conventionally manufactured polysaccharide aerogels	267:317	conventionally manufactured polysaccharide aerogels	267:317	However, the lack of macropores in conventionally manufactured polysaccharide aerogels is a limitation to their use as scaffolds for regenerative medicine.					
33259188	7	27	theme	preventive	1233:1242	arg1	effect					1244:1249	a preventive effect	1231:1249	a preventive effect	1231:1249	Zein incorporation induced remarkable changes in the mechanical performance of the end aerogel products and showed a preventive effect on the morphological changes during the storage period.					
33259188	7	28	theme	remarkable	1143:1152	arg1	changes					1154:1160	remarkable changes	1143:1160	remarkable changes	1143:1160	Zein incorporation induced remarkable changes in the mechanical performance of the end aerogel products and showed a preventive effect on the morphological changes during the storage period.					
33259188	1	29	theme	high	212:215	arg1	areas					225:229	high surface areas	212:229	high surface areas	212:229	Starch aerogels are attractive materials for biomedical applications because of their low density and high open porosity coupled with high surface areas.					
33259188	4	30	theme	population	565:574	arg1	induction					536:544	the induction	532:544	the induction of a new macropore population (1-2 μm) well integrated into the starch aerogel backbone	532:632	In this work, the induction of a new macropore population (1-2 μm) well integrated into the starch aerogel backbone was successfully achieved by the incorporation of zein as a porogen.					
33259188	3	31	theme	biomedical	469:478	arg1	purposes					480:487	biomedical purposes	469:487	biomedical purposes	469:487	Moreover, the stability under storage of polysaccharide aerogels is critical for biomedical purposes and scarcely studied so far.					
33259188	1	32	theme	surface	217:223	arg1	areas					225:229	high surface areas	212:229	high surface areas	212:229	Starch aerogels are attractive materials for biomedical applications because of their low density and high open porosity coupled with high surface areas.					
33259188	3	33	theme	polysaccharide	429:442	arg1	aerogels					444:451	polysaccharide aerogels	429:451	polysaccharide aerogels	429:451	Moreover, the stability under storage of polysaccharide aerogels is critical for biomedical purposes and scarcely studied so far.					
33259188	5	34	theme	obtained	707:714	arg1	aerogels					728:735	The obtained dual-porous aerogels	703:735	The obtained dual-porous aerogels	703:735	The obtained dual-porous aerogels were evaluated in terms of composition as well as morphological, textural, and mechanical properties.					
33259188	6	35	theme	aerogels	852:859	arg1	Stability					839:847	Stability	839:847	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions	839:1015	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	7	36	theme	storage	1291:1297	arg1	period					1299:1304	the storage period	1287:1304	the storage period	1287:1304	Zein incorporation induced remarkable changes in the mechanical performance of the end aerogel products and showed a preventive effect on the morphological changes during the storage period.					
33259188	0	37	theme	Biomedical	53:62	arg1	Applications					64:75	Biomedical Applications	53:75	Biomedical Applications	53:75	Stability Studies of Starch Aerogel Formulations for Biomedical Applications.					
33259188	6	38	theme	relative	908:915	arg1	humidity					917:924	65% relative humidity	904:924	65% relative humidity	904:924	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	1	39	theme	biomedical	123:132	arg1	applications					134:145	biomedical applications	123:145	biomedical applications	123:145	Starch aerogels are attractive materials for biomedical applications because of their low density and high open porosity coupled with high surface areas.					
33259188	5	40	theme	morphological	787:799	arg1	properties					827:836	morphological, textural, and mechanical properties	787:836	composition as well as morphological, textural, and mechanical properties	764:836	The obtained dual-porous aerogels were evaluated in terms of composition as well as morphological, textural, and mechanical properties.					
33259188	6	41	theme	%	906:906	arg1	humidity					917:924	65% relative humidity	904:924	65% relative humidity	904:924	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	6	42	from	perspectives	1102:1113	arg1	months					1043:1048	1 and 3 months	1035:1048	1 and 3 months from morphological, physicochemical, and mechanical perspectives	1035:1113	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	6	43	theme	mechanical	1091:1100	arg1	perspectives					1102:1113	morphological, physicochemical, and mechanical perspectives	1055:1113	morphological, physicochemical, and mechanical perspectives	1055:1113	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	6	44	theme	conditions	1006:1015	arg1	guideline					984:992	Harmonization guideline	970:992	Harmonization guideline of climatic conditions	970:1015	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	2	45	theme	macropores	253:262	arg1	limitation					324:333	a limitation	322:333	a limitation to their use as scaffolds for regenerative medicine	322:385	However, the lack of macropores in conventionally manufactured polysaccharide aerogels is a limitation to their use as scaffolds for regenerative medicine.					
33259188	2	45	theme	macropores	253:262	arg1	lack					245:248	the lack	241:248	the lack of macropores in conventionally manufactured polysaccharide aerogels	241:317	However, the lack of macropores in conventionally manufactured polysaccharide aerogels is a limitation to their use as scaffolds for regenerative medicine.					
33259188	6	46	theme	physicochemical	1070:1084	arg1	perspectives					1102:1113	morphological, physicochemical, and mechanical perspectives	1055:1113	morphological, physicochemical, and mechanical perspectives	1055:1113	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	6	47	theme	climatic	997:1004	arg1	conditions					1006:1015	climatic conditions	997:1015	climatic conditions	997:1015	Stability of aerogels upon storage mimicking the zone II (25 °C, 65% relative humidity) according to the International Council for Harmonization guideline of climatic conditions was checked after 1 and 3 months from morphological, physicochemical, and mechanical perspectives.					
33259188	4	48	theme	zein	684:687	arg1	incorporation					667:679	the incorporation	663:679	the incorporation of zein as a porogen	663:700	In this work, the induction of a new macropore population (1-2 μm) well integrated into the starch aerogel backbone was successfully achieved by the incorporation of zein as a porogen.					
33259188	4	49	theme	starch	610:615	arg1	backbone					625:632	the starch aerogel backbone	606:632	the starch aerogel backbone	606:632	In this work, the induction of a new macropore population (1-2 μm) well integrated into the starch aerogel backbone was successfully achieved by the incorporation of zein as a porogen.					
33259188	7	50	theme	Zein	1116:1119	arg1	incorporation					1121:1133	Zein incorporation	1116:1133	Zein incorporation	1116:1133	Zein incorporation induced remarkable changes in the mechanical performance of the end aerogel products and showed a preventive effect on the morphological changes during the storage period.					
33259188	1	51	theme	low	164:166	arg1	density					168:174	their low density	158:174	their low density	158:174	Starch aerogels are attractive materials for biomedical applications because of their low density and high open porosity coupled with high surface areas.					
33259188	7	52	theme	mechanical	1169:1178	arg1	performance					1180:1190	the mechanical performance	1165:1190	the mechanical performance of the end aerogel products	1165:1218	Zein incorporation induced remarkable changes in the mechanical performance of the end aerogel products and showed a preventive effect on the morphological changes during the storage period.					
33766357	5	0	theme	skin	731:734	arg1	model					743:747	a mouse full-thickness skin defect model	708:747	a mouse full-thickness skin defect model	708:747	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	5	1	theme	CS/SA	780:784	arg1	hydrogel					786:793	the double-network CS/SA hydrogel	761:793	the double-network CS/SA hydrogel	761:793	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	1	2	theme	wound	172:176	arg1	healing					178:184	wound healing	172:184	wound healing	172:184	Effective wound dressings are of great significance in preventing infections and promoting wound healing.					
33766357	3	3	theme	double-network	343:356	arg1	hydrogel					392:399	a double-network cross-linked polysaccharide-based hydrogel	341:399	a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA)	341:518	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	4	4	theme	swelling	589:596	arg1	ratios					598:603	suitable swelling ratios	580:603	suitable swelling ratios	580:603	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	5	5	theme	defect	736:741	arg1	model					743:747	a mouse full-thickness skin defect model	708:747	a mouse full-thickness skin defect model	708:747	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	4	6	theme	suitable	580:587	arg1	ratios					598:603	suitable swelling ratios	580:603	suitable swelling ratios	580:603	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	5	7	theme	in	686:687	arg1	Meanwhile					671:679	Meanwhile	671:679	Meanwhile	671:679	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	5	7	theme	in	686:687	arg1	studies					694:700	the in vivo studies	682:700	the in vivo studies using a mouse full-thickness skin defect model	682:747	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	6	8	theme	wound	1026:1030	arg1	dressings					1032:1040	wound dressings	1026:1040	wound dressings	1026:1040	Therefore, the functionalized double-network hydrogel should be a potential candidate as wound dressings.					
33766357	2	9	from	inadequacy	237:246	arg1	performance					269:279	mechanical performance	258:279	mechanical performance	258:279	However, most existing hydrogel dressings have an inadequacy in either mechanical performance, biological activities, or versatilities.					
33766357	2	9	from	inadequacy	237:246	arg1	activities					293:302	biological activities	282:302	biological activities	282:302	However, most existing hydrogel dressings have an inadequacy in either mechanical performance, biological activities, or versatilities.					
33766357	2	9	from	inadequacy	237:246	arg1	versatilities					308:320	versatilities	308:320	versatilities	308:320	However, most existing hydrogel dressings have an inadequacy in either mechanical performance, biological activities, or versatilities.					
33766357	1	10	theme	Effective	81:89	arg1	dressings					97:105	Effective wound dressings	81:105	Effective wound dressings	81:105	Effective wound dressings are of great significance in preventing infections and promoting wound healing.					
33766357	4	11	theme	porous	559:564	arg1	morphologies					566:577	interconnected porous morphologies	544:577	interconnected porous morphologies	544:577	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	1	12	theme	wound	91:95	arg1	dressings					97:105	Effective wound dressings	81:105	Effective wound dressings	81:105	Effective wound dressings are of great significance in preventing infections and promoting wound healing.					
33766357	0	13	theme	double-network	2:15	arg1	hydrogel					48:55	A double-network polysaccharide-based composite hydrogel	0:55	A double-network polysaccharide-based composite hydrogel for skin wound healing	0:78	A double-network polysaccharide-based composite hydrogel for skin wound healing.					
33766357	4	14	theme	interconnected	544:557	arg1	morphologies					566:577	interconnected porous morphologies	544:577	interconnected porous morphologies	544:577	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	5	15	theme	mouse	710:714	arg1	model					743:747	a mouse full-thickness skin defect model	708:747	a mouse full-thickness skin defect model	708:747	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	5	16	theme	inflammatory	853:864	arg1	process					866:872	the inflammatory process	849:872	the inflammatory process	849:872	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	3	17	theme	methacrylate	486:497	arg1	alginate					506:513	oxidized methacrylate sodium alginate	477:513	oxidized methacrylate sodium alginate (SA)	477:518	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	3	17	theme	methacrylate	486:497	arg1	SA					516:517	SA	516:517	SA	516:517	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	5	18	theme	full-thickness	716:729	arg1	model					743:747	a mouse full-thickness skin defect model	708:747	a mouse full-thickness skin defect model	708:747	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	3	19	theme	sodium	499:504	arg1	alginate					506:513	oxidized methacrylate sodium alginate	477:513	oxidized methacrylate sodium alginate (SA)	477:518	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	3	19	theme	sodium	499:504	arg1	SA					516:517	SA	516:517	SA	516:517	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	0	20	theme	composite	38:46	arg1	hydrogel					48:55	A double-network polysaccharide-based composite hydrogel	0:55	A double-network polysaccharide-based composite hydrogel for skin wound healing	0:78	A double-network polysaccharide-based composite hydrogel for skin wound healing.					
33766357	3	21	link	cross-linked	358:369	arg1	hydrogel					392:399	a double-network cross-linked polysaccharide-based hydrogel	341:399	a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA)	341:518	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	3	22	theme	carboxymethyl	445:457	arg1	chitosan					459:466	peptide-functionalized carboxymethyl chitosan	422:466	peptide-functionalized carboxymethyl chitosan (CS)	422:471	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	3	22	theme	carboxymethyl	445:457	arg1	CS					469:470	CS	469:470	CS	469:470	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	4	23	theme	favorable	643:651	arg1	biocompatibility					653:668	favorable biocompatibility	643:668	favorable biocompatibility	643:668	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	2	24	theme	most	196:199	arg1	dressings					219:227	most existing hydrogel dressings	196:227	most existing hydrogel dressings	196:227	However, most existing hydrogel dressings have an inadequacy in either mechanical performance, biological activities, or versatilities.					
33766357	1	25	theme	great	114:118	arg1	significance					120:131	great significance	114:131	great significance	114:131	Effective wound dressings are of great significance in preventing infections and promoting wound healing.					
33766357	5	26	theme	collagen	885:892	arg1	deposition					894:903	collagen deposition	885:903	collagen deposition	885:903	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	5	27	theme	wound	821:825	arg1	healing					827:833	wound healing	821:833	wound healing	821:833	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	0	28	theme	polysaccharide-based	17:36	arg1	hydrogel					48:55	A double-network polysaccharide-based composite hydrogel	0:55	A double-network polysaccharide-based composite hydrogel for skin wound healing	0:78	A double-network polysaccharide-based composite hydrogel for skin wound healing.					
33766357	3	29	theme	oxidized	477:484	arg1	alginate					506:513	oxidized methacrylate sodium alginate	477:513	oxidized methacrylate sodium alginate (SA)	477:518	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	3	29	theme	oxidized	477:484	arg1	SA					516:517	SA	516:517	SA	516:517	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	3	30	theme	peptide-functionalized	422:443	arg1	chitosan					459:466	peptide-functionalized carboxymethyl chitosan	422:466	peptide-functionalized carboxymethyl chitosan (CS)	422:471	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	3	30	theme	peptide-functionalized	422:443	arg1	CS					469:470	CS	469:470	CS	469:470	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	2	31	contain	have	229:232	arg1	dressings					219:227	most existing hydrogel dressings	196:227	most existing hydrogel dressings	196:227	However, most existing hydrogel dressings have an inadequacy in either mechanical performance, biological activities, or versatilities.					
33766357	2	31	contain	have	229:232	arg2	inadequacy					237:246	an inadequacy	234:246	an inadequacy in either mechanical performance, biological activities, or versatilities	234:320	However, most existing hydrogel dressings have an inadequacy in either mechanical performance, biological activities, or versatilities.					
33766357	0	32	theme	wound	66:70	arg1	healing					72:78	skin wound healing	61:78	skin wound healing	61:78	A double-network polysaccharide-based composite hydrogel for skin wound healing.					
33766357	0	33	theme	skin	61:64	arg1	healing					72:78	skin wound healing	61:78	skin wound healing	61:78	A double-network polysaccharide-based composite hydrogel for skin wound healing.					
33766357	4	34	theme	mechanical	616:625	arg1	properties					627:636	excellent mechanical properties	606:636	excellent mechanical properties	606:636	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	6	35	theme	double-network	967:980	arg1	candidate					1013:1021	a potential candidate	1001:1021	a potential candidate as wound dressings	1001:1040	Therefore, the functionalized double-network hydrogel should be a potential candidate as wound dressings.					
33766357	6	35	theme	double-network	967:980	arg1	hydrogel					982:989	the functionalized double-network hydrogel	948:989	the functionalized double-network hydrogel	948:989	Therefore, the functionalized double-network hydrogel should be a potential candidate as wound dressings.					
33766357	6	36	theme	potential	1003:1011	arg1	candidate					1013:1021	a potential candidate	1001:1021	a potential candidate as wound dressings	1001:1040	Therefore, the functionalized double-network hydrogel should be a potential candidate as wound dressings.					
33766357	6	36	theme	potential	1003:1011	arg1	hydrogel					982:989	the functionalized double-network hydrogel	948:989	the functionalized double-network hydrogel	948:989	Therefore, the functionalized double-network hydrogel should be a potential candidate as wound dressings.					
33766357	4	37	theme	excellent	606:614	arg1	properties					627:636	excellent mechanical properties	606:636	excellent mechanical properties	606:636	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	5	38	theme	double-network	765:778	arg1	hydrogel					786:793	the double-network CS/SA hydrogel	761:793	the double-network CS/SA hydrogel	761:793	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	6	39	theme	functionalized	952:965	arg1	candidate					1013:1021	a potential candidate	1001:1021	a potential candidate as wound dressings	1001:1040	Therefore, the functionalized double-network hydrogel should be a potential candidate as wound dressings.					
33766357	6	39	theme	functionalized	952:965	arg1	hydrogel					982:989	the functionalized double-network hydrogel	948:989	the functionalized double-network hydrogel	948:989	Therefore, the functionalized double-network hydrogel should be a potential candidate as wound dressings.					
33766357	2	40	theme	biological	282:291	arg1	activities					293:302	biological activities	282:302	biological activities	282:302	However, most existing hydrogel dressings have an inadequacy in either mechanical performance, biological activities, or versatilities.					
33766357	3	41	dep	collagen	413:420	arg1	chitosan					459:466	peptide-functionalized carboxymethyl chitosan	422:466	peptide-functionalized carboxymethyl chitosan (CS)	422:471	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	3	41	dep	collagen	413:420	arg1	CS					469:470	CS	469:470	CS	469:470	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	2	42	theme	hydrogel	210:217	arg1	dressings					219:227	most existing hydrogel dressings	196:227	most existing hydrogel dressings	196:227	However, most existing hydrogel dressings have an inadequacy in either mechanical performance, biological activities, or versatilities.					
33766357	4	43	contain	possessed	534:542	arg2	properties					627:636	excellent mechanical properties	606:636	excellent mechanical properties	606:636	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	4	43	contain	possessed	534:542	arg2	biocompatibility					653:668	favorable biocompatibility	643:668	favorable biocompatibility	643:668	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	4	43	contain	possessed	534:542	arg1	hydrogel					525:532	The hydrogel	521:532	The hydrogel	521:532	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	4	43	contain	possessed	534:542	arg2	morphologies					566:577	interconnected porous morphologies	544:577	interconnected porous morphologies	544:577	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	4	43	contain	possessed	534:542	arg2	ratios					598:603	suitable swelling ratios	580:603	suitable swelling ratios	580:603	The hydrogel possessed interconnected porous morphologies, suitable swelling ratios, excellent mechanical properties, and favorable biocompatibility.					
33766357	3	44	theme	cross-linked	358:369	arg1	hydrogel					392:399	a double-network cross-linked polysaccharide-based hydrogel	341:399	a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA)	341:518	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	2	45	theme	existing	201:208	arg1	dressings					219:227	most existing hydrogel dressings	196:227	most existing hydrogel dressings	196:227	However, most existing hydrogel dressings have an inadequacy in either mechanical performance, biological activities, or versatilities.					
33766357	5	46	dep	in	686:687	arg1	vivo					689:692	vivo	689:692	vivo	689:692	Meanwhile, the in vivo studies using a mouse full-thickness skin defect model showed that the double-network CS/SA hydrogel significantly accelerated wound healing by regulating the inflammatory process, promoting collagen deposition, and improving vascularization.					
33766357	3	47	theme	polysaccharide-based	371:390	arg1	hydrogel					392:399	a double-network cross-linked polysaccharide-based hydrogel	341:399	a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA)	341:518	Here we presented a double-network cross-linked polysaccharide-based hydrogel composed of collagen peptide-functionalized carboxymethyl chitosan (CS) and oxidized methacrylate sodium alginate (SA).					
33766357	2	48	theme	mechanical	258:267	arg1	performance					269:279	mechanical performance	258:279	mechanical performance	258:279	However, most existing hydrogel dressings have an inadequacy in either mechanical performance, biological activities, or versatilities.					
32902264	1	0	from	response	326:333	arg1	humans					338:343	humans	338:343	humans	338:343	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	10	1	from	determination	1620:1632	arg1	samples					1701:1707	complex biological samples	1682:1707	complex biological samples	1682:1707	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	10	1	from	determination	1620:1632	arg1	pharmaceuticals					1662:1676	pharmaceuticals	1662:1676	pharmaceuticals	1662:1676	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	5	2	theme	peaks	931:935	arg1	measure					895:901	The first measure	885:901	The first measure	885:901	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	5	2	theme	peaks	931:935	arg1	absence					910:916	an absence	907:916	an absence of [YHM] ion peaks	907:935	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	4	3	theme	α-galactosyl	725:736	arg1	N-glycopeptides					738:752	α-galactosyl N-glycopeptides	725:752	α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD)	725:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	3	4	theme	α-galactosyl	620:631	arg1	epitopes					633:640	α-galactosyl epitopes	620:640	α-galactosyl epitopes	620:640	Due to an identical glycan composition and molecular mass between α-galactosyl N-glycans and hybrid/high-mannose-type N-glycans, it is challenging to characterize α-galactosyl epitopes in N-glycoproteins using mass spectrometry.					
32902264	6	5	theme	second	1044:1049	arg1	criterion					1065:1073	The second complementary criterion	1040:1073	The second complementary criterion	1040:1073	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	6	5	theme	second	1044:1049	arg1	ratio					1083:1087	the ratio	1079:1087	the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I)	1079:1160	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	10	6	theme	robust	1593:1598	arg1	tool					1611:1614	a robust analytical tool	1591:1614	a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples	1591:1707	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	1	7	theme	α-galactosyl	153:164	arg1	epitope					166:172	The α-galactosyl epitope	149:172	The α-galactosyl epitope	149:172	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	1	7	theme	α-galactosyl	153:164	arg1	moiety					197:202	a terminal N-glycan moiety	177:202	a terminal N-glycan moiety of glycoproteins found in mammals	177:236	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	10	8	theme	α-galactosyl	1637:1648	arg1	epitopes					1650:1657	α-galactosyl epitopes	1637:1657	α-galactosyl epitopes in pharmaceuticals and complex biological samples	1637:1707	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	8	9	dep	mice	1368:1371	arg1	plasma					1373:1378	plasma	1373:1378	WT mice plasma	1365:1378	In Ggta1 knockout mice, we could not find any α-galactosyl N-glycoproteins identified in WT mice plasma.					
32902264	4	10	theme	liquid	782:787	arg1	chromatography					789:802	liquid chromatography	782:802	liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD)	782:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	5	11	from	absence	910:916	arg1	spectra					948:954	the HCD spectra	940:954	the HCD spectra	940:954	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	9	12	theme	sensitive	1505:1513	arg1	manner					1528:1533	a highly sensitive and specific manner	1496:1533	a highly sensitive and specific manner	1496:1533	Using this method, we could screen for α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples in a highly sensitive and specific manner.					
32902264	7	13	with	N-glycopeptides	1240:1254	arg1	accuracy					1266:1273	high accuracy	1261:1273	high accuracy	1261:1273	The measure of [I/I > 0.3] enabled a clear-cut determination of α-galactosyl N-glycopeptides with high accuracy.					
32902264	8	14	theme	α-galactosyl	1322:1333	arg1	N-glycoproteins					1335:1349	any α-galactosyl N-glycoproteins	1318:1349	any α-galactosyl N-glycoproteins identified in WT mice plasma	1318:1378	In Ggta1 knockout mice, we could not find any α-galactosyl N-glycoproteins identified in WT mice plasma.					
32902264	4	15	gly	glycoproteins	762:774	arg1	glycoproteins					762:774	mice glycoproteins	757:774	mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD)	757:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	9	16	theme	specific	1519:1526	arg1	manner					1528:1533	a highly sensitive and specific manner	1496:1533	a highly sensitive and specific manner	1496:1533	Using this method, we could screen for α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples in a highly sensitive and specific manner.					
32902264	6	17	theme	m/z	1095:1097	arg1	ion					1120:1122	an m/z 528.19 (Hex2HexNAc1) ion	1092:1122	an m/z 528.19 (Hex2HexNAc1) ion	1092:1122	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	3	18	theme	glycan	477:482	arg1	composition					484:494	identical glycan composition	467:494	identical glycan composition	467:494	Due to an identical glycan composition and molecular mass between α-galactosyl N-glycans and hybrid/high-mannose-type N-glycans, it is challenging to characterize α-galactosyl epitopes in N-glycoproteins using mass spectrometry.					
32902264	0	19	theme	Characteristic	75:88	arg1	Ions					99:102	Characteristic Fragment Ions	75:102	Characteristic Fragment Ions	75:102	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.					
32902264	1	20	theme	terminal	179:186	arg1	epitope					166:172	The α-galactosyl epitope	149:172	The α-galactosyl epitope	149:172	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	1	20	theme	terminal	179:186	arg1	moiety					197:202	a terminal N-glycan moiety	177:202	a terminal N-glycan moiety of glycoproteins found in mammals	177:236	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	8	21	theme	WT	1365:1366	arg1	mice					1368:1371	WT mice plasma	1365:1378	WT mice plasma	1365:1378	In Ggta1 knockout mice, we could not find any α-galactosyl N-glycoproteins identified in WT mice plasma.					
32902264	4	22	theme	collisional	853:863	arg1	HCD					879:881	HCD	879:881	HCD	879:881	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	4	22	theme	collisional	853:863	arg1	dissociation					865:876	higher-energy collisional dissociation	839:876	higher-energy collisional dissociation (HCD)	839:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	0	23	theme	Higher-Energy	109:121	arg1	Dissociation					135:146	Higher-Energy Collisional Dissociation	109:146	Higher-Energy Collisional Dissociation	109:146	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.					
32902264	8	24	gly	N-glycoproteins	1335:1349	arg1	N-glycoproteins					1335:1349	any α-galactosyl N-glycoproteins	1318:1349	any α-galactosyl N-glycoproteins identified in WT mice plasma	1318:1378	In Ggta1 knockout mice, we could not find any α-galactosyl N-glycoproteins identified in WT mice plasma.					
32902264	5	25	gly	N-glycopeptides	1023:1037	arg2	N-glycopeptides					1023:1037	hybrid and/or high-mannose-type N-glycopeptides	991:1037	N-glycopeptides	1023:1037	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	0	26	from	N-Glycoproteins	53:67	arg1	Identification					10:23	Selective Identification	0:23	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.	0:147	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.					
32902264	10	27	theme	complex	1682:1688	arg1	samples					1701:1707	complex biological samples	1682:1707	complex biological samples	1682:1707	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	6	28	theme	366.14	1131:1136	arg1	ion					1152:1154	m/z 366.14 (Hex1HexNAc1) ion	1127:1154	m/z 366.14 (Hex1HexNAc1) ion (I/I)	1127:1160	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	6	28	theme	366.14	1131:1136	arg1	I/I					1157:1159	I/I	1157:1159	I/I	1157:1159	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	0	29	from	Epitopes	41:48	arg1	N-Glycoproteins					53:67	N-Glycoproteins	53:67	N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation	53:146	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.					
32902264	0	30	theme	Fragment	90:97	arg1	Ions					99:102	Characteristic Fragment Ions	75:102	Characteristic Fragment Ions	75:102	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.					
32902264	4	31	theme	mass	816:819	arg1	spectrometry					821:832	tandem mass spectrometry	809:832	tandem mass spectrometry with higher-energy collisional dissociation (HCD)	809:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	0	32	theme	Selective	0:8	arg1	Identification					10:23	Selective Identification	0:23	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.	0:147	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.					
32902264	2	33	theme	organ	438:442	arg1	transplants					444:454	organ transplants	438:454	organ transplants	438:454	Accordingly, it is necessary to analyze the α-galactosyl structure in biopharmaceuticals or organ transplants.					
32902264	0	34	theme	Epitopes	41:48	arg1	Identification					10:23	Selective Identification	0:23	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.	0:147	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.					
32902264	7	35	theme	>	1183:1183	arg1	measure					1167:1173	The measure	1163:1173	The measure of [I/I > 0.3]	1163:1188	The measure of [I/I > 0.3] enabled a clear-cut determination of α-galactosyl N-glycopeptides with high accuracy.					
32902264	10	36	from	pharmaceuticals	1662:1676	arg1	determination					1620:1632	determination	1620:1632	determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples	1620:1707	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	2	37	from	structure	403:411	arg1	biopharmaceuticals					416:433	biopharmaceuticals	416:433	biopharmaceuticals	416:433	Accordingly, it is necessary to analyze the α-galactosyl structure in biopharmaceuticals or organ transplants.					
32902264	2	37	from	structure	403:411	arg1	transplants					444:454	organ transplants	438:454	organ transplants	438:454	Accordingly, it is necessary to analyze the α-galactosyl structure in biopharmaceuticals or organ transplants.					
32902264	10	38	from	samples	1701:1707	arg1	determination					1620:1632	determination	1620:1632	determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples	1620:1707	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	10	39	theme	biological	1690:1699	arg1	samples					1701:1707	complex biological samples	1682:1707	complex biological samples	1682:1707	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	5	40	dep	absence	910:916	arg1	observed					979:986	observed	979:986	was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides	963:1037	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	1	41	theme	glycoproteins	207:219	arg1	epitope					166:172	The α-galactosyl epitope	149:172	The α-galactosyl epitope	149:172	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	1	41	theme	glycoproteins	207:219	arg1	moiety					197:202	a terminal N-glycan moiety	177:202	a terminal N-glycan moiety of glycoproteins found in mammals	177:236	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	3	42	dep	composition	484:494	arg1	an					464:465	an	464:465	an	464:465	Due to an identical glycan composition and molecular mass between α-galactosyl N-glycans and hybrid/high-mannose-type N-glycans, it is challenging to characterize α-galactosyl epitopes in N-glycoproteins using mass spectrometry.					
32902264	4	43	with	chromatography	789:802	arg1	spectrometry					821:832	tandem mass spectrometry	809:832	tandem mass spectrometry with higher-energy collisional dissociation (HCD)	809:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	1	44	gly	glycoproteins	207:219	arg1	glycoproteins					207:219	glycoproteins	207:219	glycoproteins found in mammals	207:236	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	3	45	theme	α-galactosyl	523:534	arg1	N-glycans					536:544	α-galactosyl N-glycans	523:544	α-galactosyl N-glycans	523:544	Due to an identical glycan composition and molecular mass between α-galactosyl N-glycans and hybrid/high-mannose-type N-glycans, it is challenging to characterize α-galactosyl epitopes in N-glycoproteins using mass spectrometry.					
32902264	6	46	theme	Hex1HexNAc1	1139:1149	arg1	ion					1152:1154	m/z 366.14 (Hex1HexNAc1) ion	1127:1154	m/z 366.14 (Hex1HexNAc1) ion (I/I)	1127:1160	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	6	46	theme	Hex1HexNAc1	1139:1149	arg1	I/I					1157:1159	I/I	1157:1159	I/I	1157:1159	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	4	47	from	N-glycopeptides	738:752	arg1	glycoproteins					762:774	mice glycoproteins	757:774	mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD)	757:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	8	48	theme	knockout	1285:1292	arg1	mice					1294:1297	Ggta1 knockout mice	1279:1297	Ggta1 knockout mice	1279:1297	In Ggta1 knockout mice, we could not find any α-galactosyl N-glycoproteins identified in WT mice plasma.					
32902264	5	49	theme	ion	927:929	arg1	peaks					931:935	[YHM] ion peaks	921:935	[YHM] ion peaks	921:935	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	10	50	from	epitopes	1650:1657	arg1	samples					1701:1707	complex biological samples	1682:1707	complex biological samples	1682:1707	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	10	50	from	epitopes	1650:1657	arg1	pharmaceuticals					1662:1676	pharmaceuticals	1662:1676	pharmaceuticals	1662:1676	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	6	51	theme	complementary	1051:1063	arg1	criterion					1065:1073	The second complementary criterion	1040:1073	The second complementary criterion	1040:1073	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	6	51	theme	complementary	1051:1063	arg1	ratio					1083:1087	the ratio	1079:1087	the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I)	1079:1160	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	8	52	theme	Ggta1	1279:1283	arg1	mice					1294:1297	Ggta1 knockout mice	1279:1297	Ggta1 knockout mice	1279:1297	In Ggta1 knockout mice, we could not find any α-galactosyl N-glycoproteins identified in WT mice plasma.					
32902264	10	53	theme	analytical	1600:1609	arg1	tool					1611:1614	a robust analytical tool	1591:1614	a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples	1591:1707	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	9	54	theme	plasma	1478:1483	arg1	samples					1485:1491	plasma samples	1478:1491	plasma samples	1478:1491	Using this method, we could screen for α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples in a highly sensitive and specific manner.					
32902264	3	55	gly	N-glycoproteins	645:659	arg1	N-glycoproteins					645:659	N-glycoproteins	645:659	N-glycoproteins using mass spectrometry	645:683	Due to an identical glycan composition and molecular mass between α-galactosyl N-glycans and hybrid/high-mannose-type N-glycans, it is challenging to characterize α-galactosyl epitopes in N-glycoproteins using mass spectrometry.					
32902264	10	56	theme	epitopes	1650:1657	arg1	determination					1620:1632	determination	1620:1632	determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples	1620:1707	Conclusively, we suggest that this method will provide a robust analytical tool for determination of α-galactosyl epitopes in pharmaceuticals and complex biological samples.					
32902264	5	57	theme	[YHM	921:924	arg1	peaks					931:935	[YHM] ion peaks	921:935	[YHM] ion peaks	921:935	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	4	58	with	spectrometry	821:832	arg1	HCD					879:881	HCD	879:881	HCD	879:881	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	4	58	with	spectrometry	821:832	arg1	dissociation					865:876	higher-energy collisional dissociation	839:876	higher-energy collisional dissociation (HCD)	839:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	4	59	gly	N-glycopeptides	738:752	arg2	N-glycopeptides					738:752	α-galactosyl N-glycopeptides	725:752	α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD)	725:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	1	60	located	found	221:225	arg2	glycoproteins					207:219	glycoproteins	207:219	glycoproteins found in mammals	207:236	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	1	60	located	found	221:225	arg1	mammals					230:236	mammals	230:236	mammals	230:236	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	9	61	dep	mice	1454:1457	arg1	samples					1485:1491	plasma samples	1478:1491	plasma samples	1478:1491	Using this method, we could screen for α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples in a highly sensitive and specific manner.					
32902264	9	61	dep	mice	1454:1457	arg1	lungs					1467:1471	lungs	1467:1471	lungs	1467:1471	Using this method, we could screen for α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples in a highly sensitive and specific manner.					
32902264	9	61	dep	mice	1454:1457	arg1	spleen					1459:1464	spleen	1459:1464	spleen	1459:1464	Using this method, we could screen for α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples in a highly sensitive and specific manner.					
32902264	6	62	theme	528.19	1099:1104	arg1	ion					1120:1122	an m/z 528.19 (Hex2HexNAc1) ion	1092:1122	an m/z 528.19 (Hex2HexNAc1) ion	1092:1122	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	7	63	theme	high	1261:1264	arg1	accuracy					1266:1273	high accuracy	1261:1273	high accuracy	1261:1273	The measure of [I/I > 0.3] enabled a clear-cut determination of α-galactosyl N-glycopeptides with high accuracy.					
32902264	3	64	theme	mass	667:670	arg1	spectrometry					672:683	mass spectrometry	667:683	mass spectrometry	667:683	Due to an identical glycan composition and molecular mass between α-galactosyl N-glycans and hybrid/high-mannose-type N-glycans, it is challenging to characterize α-galactosyl epitopes in N-glycoproteins using mass spectrometry.					
32902264	5	65	theme	HCD	944:946	arg1	spectra					948:954	the HCD spectra	940:954	the HCD spectra	940:954	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	7	66	theme	α-galactosyl	1227:1238	arg1	N-glycopeptides					1240:1254	α-galactosyl N-glycopeptides	1227:1254	α-galactosyl N-glycopeptides with high accuracy	1227:1273	The measure of [I/I > 0.3] enabled a clear-cut determination of α-galactosyl N-glycopeptides with high accuracy.					
32902264	7	67	theme	N-glycopeptides	1240:1254	arg1	determination					1210:1222	a clear-cut determination	1198:1222	a clear-cut determination of α-galactosyl N-glycopeptides with high accuracy	1198:1273	The measure of [I/I > 0.3] enabled a clear-cut determination of α-galactosyl N-glycopeptides with high accuracy.					
32902264	1	68	theme	N-glycan	188:195	arg1	epitope					166:172	The α-galactosyl epitope	149:172	The α-galactosyl epitope	149:172	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	1	68	theme	N-glycan	188:195	arg1	moiety					197:202	a terminal N-glycan moiety	177:202	a terminal N-glycan moiety of glycoproteins found in mammals	177:236	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	0	69	from	Identification	10:23	arg1	N-Glycoproteins					53:67	N-Glycoproteins	53:67	N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation	53:146	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.					
32902264	4	70	theme	mice	757:760	arg1	glycoproteins					762:774	mice glycoproteins	757:774	mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD)	757:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	0	71	theme	Collisional	123:133	arg1	Dissociation					135:146	Higher-Energy Collisional Dissociation	109:146	Higher-Energy Collisional Dissociation	109:146	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.					
32902264	5	72	located	observed	979:986	arg1	N-glycopeptides					1023:1037	hybrid and/or high-mannose-type N-glycopeptides	991:1037	N-glycopeptides	1023:1037	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	5	72	located	observed	979:986	arg2	which					957:961	which	957:961	which	957:961	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	1	73	from	moiety	197:202	arg1	humans					248:253	humans	248:253	humans	248:253	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	4	74	theme	higher-energy	839:851	arg1	HCD					879:881	HCD	879:881	HCD	879:881	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	4	74	theme	higher-energy	839:851	arg1	dissociation					865:876	higher-energy collisional dissociation	839:876	higher-energy collisional dissociation (HCD)	839:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	7	75	theme	clear-cut	1200:1208	arg1	determination					1210:1222	a clear-cut determination	1198:1222	a clear-cut determination of α-galactosyl N-glycopeptides with high accuracy	1198:1273	The measure of [I/I > 0.3] enabled a clear-cut determination of α-galactosyl N-glycopeptides with high accuracy.					
32902264	2	76	theme	α-galactosyl	390:401	arg1	structure					403:411	the α-galactosyl structure	386:411	the α-galactosyl structure in biopharmaceuticals or organ transplants	386:454	Accordingly, it is necessary to analyze the α-galactosyl structure in biopharmaceuticals or organ transplants.					
32902264	5	77	theme	first	889:893	arg1	measure					895:901	The first measure	885:901	The first measure	885:901	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	5	77	theme	first	889:893	arg1	absence					910:916	an absence	907:916	an absence of [YHM] ion peaks	907:935	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	5	78	theme	hybrid	991:996	arg1	N-glycopeptides					1023:1037	hybrid and/or high-mannose-type N-glycopeptides	991:1037	N-glycopeptides	1023:1037	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	6	79	theme	m/z	1127:1129	arg1	ion					1152:1154	m/z 366.14 (Hex1HexNAc1) ion	1127:1154	m/z 366.14 (Hex1HexNAc1) ion (I/I)	1127:1160	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	6	79	theme	m/z	1127:1129	arg1	I/I					1157:1159	I/I	1157:1159	I/I	1157:1159	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	4	80	theme	tandem	809:814	arg1	spectrometry					821:832	tandem mass spectrometry	809:832	tandem mass spectrometry with higher-energy collisional dissociation (HCD)	809:882	Here, we describe a method to identify α-galactosyl N-glycopeptides in mice glycoproteins using liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation (HCD).					
32902264	0	81	theme	α-Galactosyl	28:39	arg1	Epitopes					41:48	α-Galactosyl Epitopes	28:48	α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation	28:146	Selective Identification of α-Galactosyl Epitopes in N-Glycoproteins Using Characteristic Fragment Ions from Higher-Energy Collisional Dissociation.					
32902264	9	82	from	mice	1454:1457	arg1	N-glycoproteins					1433:1447	α-galactosyl N-glycoproteins	1420:1447	α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples	1420:1491	Using this method, we could screen for α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples in a highly sensitive and specific manner.					
32902264	5	83	theme	high-mannose-type	1005:1021	arg1	N-glycopeptides					1023:1037	hybrid and/or high-mannose-type N-glycopeptides	991:1037	N-glycopeptides	1023:1037	The first measure was an absence of [YHM] ion peaks in the HCD spectra, which was exclusively observed in hybrid and/or high-mannose-type N-glycopeptides.					
32902264	6	84	theme	ion	1120:1122	arg1	criterion					1065:1073	The second complementary criterion	1040:1073	The second complementary criterion	1040:1073	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	6	84	theme	ion	1120:1122	arg1	ratio					1083:1087	the ratio	1079:1087	the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I)	1079:1160	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	7	85	gly	N-glycopeptides	1240:1254	arg2	N-glycopeptides					1240:1254	α-galactosyl N-glycopeptides	1227:1254	α-galactosyl N-glycopeptides with high accuracy	1227:1273	The measure of [I/I > 0.3] enabled a clear-cut determination of α-galactosyl N-glycopeptides with high accuracy.					
32902264	7	86	theme	[I/I	1178:1181	arg1	>					1183:1183	[I/I > 0.3]	1178:1188	[I/I > 0.3]	1178:1188	The measure of [I/I > 0.3] enabled a clear-cut determination of α-galactosyl N-glycopeptides with high accuracy.					
32902264	6	87	theme	Hex2HexNAc1	1107:1117	arg1	ion					1120:1122	an m/z 528.19 (Hex2HexNAc1) ion	1092:1122	an m/z 528.19 (Hex2HexNAc1) ion	1092:1122	The second complementary criterion was the ratio of an m/z 528.19 (Hex2HexNAc1) ion to m/z 366.14 (Hex1HexNAc1) ion (I/I).					
32902264	3	88	theme	identical	467:475	arg1	composition					484:494	identical glycan composition	467:494	identical glycan composition	467:494	Due to an identical glycan composition and molecular mass between α-galactosyl N-glycans and hybrid/high-mannose-type N-glycans, it is challenging to characterize α-galactosyl epitopes in N-glycoproteins using mass spectrometry.					
32902264	1	89	theme	immunogenic	314:324	arg1	response					326:333	an immunogenic response	311:333	an immunogenic response in humans	311:343	The α-galactosyl epitope is a terminal N-glycan moiety of glycoproteins found in mammals except in humans, and thus, it is recognized as an antigen that provokes an immunogenic response in humans.					
32902264	9	90	gly	N-glycoproteins	1433:1447	arg1	N-glycoproteins					1433:1447	α-galactosyl N-glycoproteins	1420:1447	α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples	1420:1491	Using this method, we could screen for α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples in a highly sensitive and specific manner.					
32902264	3	91	theme	molecular	500:508	arg1	mass					510:513	molecular mass	500:513	molecular mass	500:513	Due to an identical glycan composition and molecular mass between α-galactosyl N-glycans and hybrid/high-mannose-type N-glycans, it is challenging to characterize α-galactosyl epitopes in N-glycoproteins using mass spectrometry.					
32902264	3	92	theme	hybrid/high-mannose-type	550:573	arg1	N-glycans					575:583	hybrid/high-mannose-type N-glycans	550:583	hybrid/high-mannose-type N-glycans	550:583	Due to an identical glycan composition and molecular mass between α-galactosyl N-glycans and hybrid/high-mannose-type N-glycans, it is challenging to characterize α-galactosyl epitopes in N-glycoproteins using mass spectrometry.					
32902264	9	93	theme	α-galactosyl	1420:1431	arg1	N-glycoproteins					1433:1447	α-galactosyl N-glycoproteins	1420:1447	α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples	1420:1491	Using this method, we could screen for α-galactosyl N-glycoproteins from mice spleen, lungs, and plasma samples in a highly sensitive and specific manner.					
33369405	8	0	theme	different	1592:1600	arg1	drugs					1606:1610	two different GAG drugs	1588:1610	two different GAG drugs	1588:1610	The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide, were demonstrated.					
33369405	8	0	theme	different	1592:1600	arg1	sulodexide					1625:1634	sulodexide	1625:1634	sulodexide	1625:1634	The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide, were demonstrated.					
33369405	8	0	theme	different	1592:1600	arg1	heparin					1613:1619	heparin	1613:1619	heparin	1613:1619	The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide, were demonstrated.					
33369405	3	1	theme	Numerous	527:534	arg1	methods					536:542	Numerous methods	527:542	Numerous methods	527:542	Numerous methods have been developed to characterize the structure of pharmaceutical GAGs, including intravenously or subcutaneously administered heparin and orally administered sulodexide.					
33369405	9	2	theme	vague	1816:1820	arg1	picture					1822:1828	a vague picture	1814:1828	a vague picture of all chains	1814:1842	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	9	2	theme	vague	1816:1820	arg1	mixture					1849:1855	a mixture	1847:1855	a mixture	1847:1855	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	9	2	theme	vague	1816:1820	arg1	fundamental					1867:1877	fundamental	1867:1877	fundamental	1867:1877	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	6	3	theme	in-gel	1099:1104	arg1	digestion					1106:1114	an in-gel digestion	1096:1114	an in-gel digestion procedure	1096:1124	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	0	4	theme	In-Depth	83:90	arg1	Analysis					92:99	In-Depth Analysis	83:99	In-Depth Analysis of Glycosaminoglycan Drugs	83:126	Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.					
33369405	9	5	theme	robust	1691:1696	arg1	tool					1698:1701	the first robust tool	1681:1701	the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs	1681:1975	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	9	6	theme	chains	1753:1758	arg1	structure					1729:1737	the structure	1725:1737	the structure of larger GAG chains with more biological importance	1725:1790	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	0	7	theme	Glycosaminoglycan	104:120	arg1	Drugs					122:126	Glycosaminoglycan Drugs	104:126	Glycosaminoglycan Drugs	104:126	Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.					
33369405	7	8	theme	chain	1482:1486	arg1	length					1488:1493	chain length	1482:1493	chain length	1482:1493	Both procedures are compatible with liquid chromatography-tandem mass spectrometry, and structural information, such as disaccharide composition and chain length, can be revealed for each GAG fraction.					
33369405	9	9	theme	GAG	1749:1751	arg1	chains					1753:1758	larger GAG chains	1742:1758	larger GAG chains	1742:1758	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	6	10	theme	anion	1223:1227	arg1	procedure					1254:1262	a strong anion exchange ultrafiltration procedure	1214:1262	a strong anion exchange ultrafiltration procedure	1214:1262	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	5	11	theme	structures	1040:1049	arg1	view					1028:1031	a panoramic view	1016:1031	a panoramic view of the structures of pharmaceutical GAGs	1016:1072	We have established two novel electrophoresis-mass spectrometry methods to provide a panoramic view of the structures of pharmaceutical GAGs.					
33369405	7	12	with	compatible	1353:1362	arg1	spectrometry					1403:1414	liquid chromatography-tandem mass spectrometry	1369:1414	liquid chromatography-tandem mass spectrometry	1369:1414	Both procedures are compatible with liquid chromatography-tandem mass spectrometry, and structural information, such as disaccharide composition and chain length, can be revealed for each GAG fraction.					
33369405	7	13	theme	liquid	1369:1374	arg1	spectrometry					1403:1414	liquid chromatography-tandem mass spectrometry	1369:1414	liquid chromatography-tandem mass spectrometry	1369:1414	Both procedures are compatible with liquid chromatography-tandem mass spectrometry, and structural information, such as disaccharide composition and chain length, can be revealed for each GAG fraction.					
33369405	5	14	theme	panoramic	1018:1026	arg1	view					1028:1031	a panoramic view	1016:1031	a panoramic view of the structures of pharmaceutical GAGs	1016:1072	We have established two novel electrophoresis-mass spectrometry methods to provide a panoramic view of the structures of pharmaceutical GAGs.					
33369405	7	15	theme	structural	1421:1430	arg1	information					1432:1442	structural information	1421:1442	structural information	1421:1442	Both procedures are compatible with liquid chromatography-tandem mass spectrometry, and structural information, such as disaccharide composition and chain length, can be revealed for each GAG fraction.					
33369405	5	16	theme	electrophoresis-mass	963:982	arg1	methods					997:1003	two novel electrophoresis-mass spectrometry methods	953:1003	two novel electrophoresis-mass spectrometry methods	953:1003	We have established two novel electrophoresis-mass spectrometry methods to provide a panoramic view of the structures of pharmaceutical GAGs.					
33369405	0	17	theme	Drugs	122:126	arg1	Analysis					92:99	In-Depth Analysis	83:99	In-Depth Analysis of Glycosaminoglycan Drugs	83:126	Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.					
33369405	9	18	theme	structure-activity	1908:1925	arg1	relationship					1927:1938	structure-activity relationship	1908:1938	structure-activity relationship	1908:1938	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	6	19	theme	exchange	1229:1236	arg1	procedure					1254:1262	a strong anion exchange ultrafiltration procedure	1214:1262	a strong anion exchange ultrafiltration procedure	1214:1262	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	4	20	theme	longer-chain	838:849	arg1	polysaccharides					851:865	longer-chain polysaccharides	838:865	longer-chain polysaccharides	838:865	However, most currently available methods only focus on the oligosaccharide portion or analyze the whole mixture because longer-chain polysaccharides are extremely difficult to resolve by chromatographic separation.					
33369405	5	21	theme	novel	957:961	arg1	methods					997:1003	two novel electrophoresis-mass spectrometry methods	953:1003	two novel electrophoresis-mass spectrometry methods	953:1003	We have established two novel electrophoresis-mass spectrometry methods to provide a panoramic view of the structures of pharmaceutical GAGs.					
33369405	0	22	theme	Liquid	9:14	arg1	Chromatography					16:29	Coupling Liquid Chromatography	0:29	Coupling Liquid Chromatography	0:29	Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.					
33369405	9	23	theme	larger	1742:1747	arg1	chains					1753:1758	larger GAG chains	1742:1758	larger GAG chains	1742:1758	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	6	24	theme	first	1082:1086	arg1	method					1088:1093	the first method	1078:1093	the first method	1078:1093	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	3	25	theme	pharmaceutical	597:610	arg1	sulodexide					705:714	orally administered sulodexide	685:714	orally administered sulodexide	685:714	Numerous methods have been developed to characterize the structure of pharmaceutical GAGs, including intravenously or subcutaneously administered heparin and orally administered sulodexide.					
33369405	3	25	theme	pharmaceutical	597:610	arg1	heparin					673:679	intravenously or subcutaneously administered heparin	628:679	intravenously or subcutaneously administered heparin	628:679	Numerous methods have been developed to characterize the structure of pharmaceutical GAGs, including intravenously or subcutaneously administered heparin and orally administered sulodexide.					
33369405	3	25	theme	pharmaceutical	597:610	arg1	GAGs					612:615	pharmaceutical GAGs	597:615	pharmaceutical GAGs	597:615	Numerous methods have been developed to characterize the structure of pharmaceutical GAGs, including intravenously or subcutaneously administered heparin and orally administered sulodexide.					
33369405	6	26	theme	polyacrylamide	1165:1178	arg1	gels					1180:1183	the polyacrylamide gels	1161:1183	the polyacrylamide gels	1161:1183	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	2	27	theme	coagulation	398:408	arg1	factors					410:416	multiple coagulation factors	389:416	multiple coagulation factors	389:416	GAGs interrupt the coagulation process by interacting with multiple coagulation factors through defined sequences within their linear and negatively charged chains, which are not fully elucidated.					
33369405	0	28	theme	Coupling	0:7	arg1	Chromatography					16:29	Coupling Liquid Chromatography	0:29	Coupling Liquid Chromatography	0:29	Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.					
33369405	1	29	theme	thrombosis	277:286	arg1	field					268:272	the field	264:272	the field of thrombosis	264:286	Glycosaminoglycans (GAGs) contribute to the treatment of many human diseases, especially in the field of thrombosis, because of their anticoagulant activity.					
33369405	9	30	dep	relationship	1927:1938	arg1	the					1904:1906	the	1904:1906	the	1904:1906	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	3	31	theme	GAGs	612:615	arg1	structure					584:592	the structure	580:592	the structure of pharmaceutical GAGs, including intravenously or subcutaneously administered heparin and orally administered sulodexide	580:714	Numerous methods have been developed to characterize the structure of pharmaceutical GAGs, including intravenously or subcutaneously administered heparin and orally administered sulodexide.					
33369405	8	32	from	applications	1539:1550	arg1	analysis					1576:1583	analysis	1576:1583	analysis of two different GAG drugs, heparin and sulodexide,	1576:1635	The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide, were demonstrated.					
33369405	2	33	theme	multiple	389:396	arg1	factors					410:416	multiple coagulation factors	389:416	multiple coagulation factors	389:416	GAGs interrupt the coagulation process by interacting with multiple coagulation factors through defined sequences within their linear and negatively charged chains, which are not fully elucidated.					
33369405	9	34	theme	biological	1770:1779	arg1	importance					1781:1790	more biological importance	1765:1790	more biological importance	1765:1790	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	7	35	theme	GAG	1521:1523	arg1	fraction					1525:1532	each GAG fraction	1516:1532	each GAG fraction	1516:1532	Both procedures are compatible with liquid chromatography-tandem mass spectrometry, and structural information, such as disaccharide composition and chain length, can be revealed for each GAG fraction.					
33369405	4	36	theme	whole	816:820	arg1	mixture					822:828	the whole mixture	812:828	the whole mixture	812:828	However, most currently available methods only focus on the oligosaccharide portion or analyze the whole mixture because longer-chain polysaccharides are extremely difficult to resolve by chromatographic separation.					
33369405	9	37	theme	quality	1944:1950	arg1	control					1952:1958	quality control	1944:1958	quality control	1944:1958	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	2	38	theme	linear	457:462	arg1	chains					487:492	their linear and negatively charged chains	451:492	their linear and negatively charged chains	451:492	GAGs interrupt the coagulation process by interacting with multiple coagulation factors through defined sequences within their linear and negatively charged chains, which are not fully elucidated.					
33369405	5	39	theme	GAGs	1069:1072	arg1	structures					1040:1049	the structures	1036:1049	the structures of pharmaceutical GAGs	1036:1072	We have established two novel electrophoresis-mass spectrometry methods to provide a panoramic view of the structures of pharmaceutical GAGs.					
33369405	6	40	theme	ultrafiltration	1238:1252	arg1	procedure					1254:1262	a strong anion exchange ultrafiltration procedure	1214:1262	a strong anion exchange ultrafiltration procedure	1214:1262	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	0	41	theme	Mass	42:45	arg1	Spectrometry					47:58	Tandem Mass Spectrometry	35:58	Tandem Mass Spectrometry	35:58	Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.					
33369405	9	42	theme	current	1660:1666	arg1	study					1668:1672	The current study	1656:1672	The current study	1656:1672	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	0	43	theme	Multicomponent	145:158	arg1	Sulodexide					160:169	the Multicomponent Sulodexide	141:169	the Multicomponent Sulodexide	141:169	Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.					
33369405	9	44	theme	first	1685:1689	arg1	tool					1698:1701	the first robust tool	1681:1701	the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs	1681:1975	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	5	45	theme	spectrometry	984:995	arg1	methods					997:1003	two novel electrophoresis-mass spectrometry methods	953:1003	two novel electrophoresis-mass spectrometry methods	953:1003	We have established two novel electrophoresis-mass spectrometry methods to provide a panoramic view of the structures of pharmaceutical GAGs.					
33369405	0	46	theme	Tandem	35:40	arg1	Spectrometry					47:58	Tandem Mass Spectrometry	35:58	Tandem Mass Spectrometry	35:58	Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.					
33369405	8	47	theme	methods	1565:1571	arg1	applications					1539:1550	The applications	1535:1550	The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide,	1535:1635	The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide, were demonstrated.					
33369405	9	48	theme	GAG	1967:1969	arg1	drugs					1971:1975	the GAG drugs	1963:1975	the GAG drugs	1963:1975	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	1	49	theme	many	229:232	arg1	diseases					240:247	many human diseases	229:247	many human diseases	229:247	Glycosaminoglycans (GAGs) contribute to the treatment of many human diseases, especially in the field of thrombosis, because of their anticoagulant activity.					
33369405	1	50	theme	anticoagulant	306:318	arg1	activity					320:327	their anticoagulant activity	300:327	their anticoagulant activity	300:327	Glycosaminoglycans (GAGs) contribute to the treatment of many human diseases, especially in the field of thrombosis, because of their anticoagulant activity.					
33369405	7	51	theme	disaccharide	1453:1464	arg1	composition					1466:1476	disaccharide composition	1453:1476	disaccharide composition	1453:1476	Both procedures are compatible with liquid chromatography-tandem mass spectrometry, and structural information, such as disaccharide composition and chain length, can be revealed for each GAG fraction.					
33369405	3	52	theme	administered	692:703	arg1	sulodexide					705:714	orally administered sulodexide	685:714	orally administered sulodexide	685:714	Numerous methods have been developed to characterize the structure of pharmaceutical GAGs, including intravenously or subcutaneously administered heparin and orally administered sulodexide.					
33369405	6	53	theme	agarose	1319:1325	arg1	gels					1327:1330	the agarose gels	1315:1330	the agarose gels	1315:1330	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	2	54	theme	coagulation	349:359	arg1	process					361:367	the coagulation process	345:367	the coagulation process	345:367	GAGs interrupt the coagulation process by interacting with multiple coagulation factors through defined sequences within their linear and negatively charged chains, which are not fully elucidated.					
33369405	8	55	theme	drugs	1606:1610	arg1	analysis					1576:1583	analysis	1576:1583	analysis of two different GAG drugs, heparin and sulodexide,	1576:1635	The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide, were demonstrated.					
33369405	4	56	theme	chromatographic	905:919	arg1	separation					921:930	chromatographic separation	905:930	chromatographic separation	905:930	However, most currently available methods only focus on the oligosaccharide portion or analyze the whole mixture because longer-chain polysaccharides are extremely difficult to resolve by chromatographic separation.					
33369405	7	57	theme	chromatography-tandem	1376:1396	arg1	spectrometry					1403:1414	liquid chromatography-tandem mass spectrometry	1369:1414	liquid chromatography-tandem mass spectrometry	1369:1414	Both procedures are compatible with liquid chromatography-tandem mass spectrometry, and structural information, such as disaccharide composition and chain length, can be revealed for each GAG fraction.					
33369405	7	58	theme	mass	1398:1401	arg1	spectrometry					1403:1414	liquid chromatography-tandem mass spectrometry	1369:1414	liquid chromatography-tandem mass spectrometry	1369:1414	Both procedures are compatible with liquid chromatography-tandem mass spectrometry, and structural information, such as disaccharide composition and chain length, can be revealed for each GAG fraction.					
33369405	6	59	theme	multiple	1289:1296	arg1	species					1302:1308	multiple GAG species	1289:1308	multiple GAG species	1289:1308	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	4	60	theme	available	741:749	arg1	methods					751:757	most currently available methods	726:757	most currently available methods	726:757	However, most currently available methods only focus on the oligosaccharide portion or analyze the whole mixture because longer-chain polysaccharides are extremely difficult to resolve by chromatographic separation.					
33369405	5	61	theme	pharmaceutical	1054:1067	arg1	GAGs					1069:1072	pharmaceutical GAGs	1054:1072	pharmaceutical GAGs	1054:1072	We have established two novel electrophoresis-mass spectrometry methods to provide a panoramic view of the structures of pharmaceutical GAGs.					
33369405	1	62	theme	human	234:238	arg1	diseases					240:247	many human diseases	229:247	many human diseases	229:247	Glycosaminoglycans (GAGs) contribute to the treatment of many human diseases, especially in the field of thrombosis, because of their anticoagulant activity.					
33369405	0	63	dep	Chromatography	16:29	arg1	Heparin					129:135	Heparin	129:135	Heparin	129:135	Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.					
33369405	0	63	dep	Chromatography	16:29	arg1	Sulodexide					160:169	the Multicomponent Sulodexide	141:169	the Multicomponent Sulodexide	141:169	Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.					
33369405	4	64	theme	oligosaccharide	777:791	arg1	portion					793:799	the oligosaccharide portion	773:799	the oligosaccharide portion	773:799	However, most currently available methods only focus on the oligosaccharide portion or analyze the whole mixture because longer-chain polysaccharides are extremely difficult to resolve by chromatographic separation.					
33369405	6	65	theme	strong	1216:1221	arg1	procedure					1254:1262	a strong anion exchange ultrafiltration procedure	1214:1262	a strong anion exchange ultrafiltration procedure	1214:1262	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	1	66	theme	diseases	240:247	arg1	treatment					216:224	the treatment	212:224	the treatment of many human diseases	212:247	Glycosaminoglycans (GAGs) contribute to the treatment of many human diseases, especially in the field of thrombosis, because of their anticoagulant activity.					
33369405	9	67	theme	chains	1837:1842	arg1	picture					1822:1828	a vague picture	1814:1828	a vague picture of all chains	1814:1842	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	9	67	theme	chains	1837:1842	arg1	mixture					1849:1855	a mixture	1847:1855	a mixture	1847:1855	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	9	67	theme	chains	1837:1842	arg1	fundamental					1867:1877	fundamental	1867:1877	fundamental	1867:1877	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	2	68	theme	defined	426:432	arg1	sequences					434:442	defined sequences	426:442	defined sequences within their linear and negatively charged chains, which are not fully elucidated	426:524	GAGs interrupt the coagulation process by interacting with multiple coagulation factors through defined sequences within their linear and negatively charged chains, which are not fully elucidated.					
33369405	6	69	theme	GAG	1298:1300	arg1	species					1302:1308	multiple GAG species	1289:1308	multiple GAG species	1289:1308	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	6	70	theme	second	1199:1204	arg1	method					1206:1211	the second method	1195:1211	the second method	1195:1211	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	6	71	from	gels	1327:1330	arg1	extract					1281:1287	extract	1281:1287	extract	1281:1287	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
33369405	9	72	with	structure	1729:1737	arg1	importance					1781:1790	more biological importance	1765:1790	more biological importance	1765:1790	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	8	73	theme	GAG	1602:1604	arg1	drugs					1606:1610	two different GAG drugs	1588:1610	two different GAG drugs	1588:1610	The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide, were demonstrated.					
33369405	8	73	theme	GAG	1602:1604	arg1	sulodexide					1625:1634	sulodexide	1625:1634	sulodexide	1625:1634	The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide, were demonstrated.					
33369405	8	73	theme	GAG	1602:1604	arg1	heparin					1613:1619	heparin	1613:1619	heparin	1613:1619	The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide, were demonstrated.					
33369405	3	74	theme	administered	660:671	arg1	heparin					673:679	intravenously or subcutaneously administered heparin	628:679	intravenously or subcutaneously administered heparin	628:679	Numerous methods have been developed to characterize the structure of pharmaceutical GAGs, including intravenously or subcutaneously administered heparin and orally administered sulodexide.					
33369405	2	75	theme	charged	479:485	arg1	chains					487:492	their linear and negatively charged chains	451:492	their linear and negatively charged chains	451:492	GAGs interrupt the coagulation process by interacting with multiple coagulation factors through defined sequences within their linear and negatively charged chains, which are not fully elucidated.					
33369405	9	76	theme	drugs	1971:1975	arg1	relationship					1927:1938	structure-activity relationship	1908:1938	structure-activity relationship	1908:1938	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	9	76	theme	drugs	1971:1975	arg1	control					1952:1958	quality control	1944:1958	quality control	1944:1958	The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.					
33369405	6	77	theme	digestion	1106:1114	arg1	procedure					1116:1124	an in-gel digestion procedure	1096:1124	an in-gel digestion procedure	1096:1124	In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels.					
34087714	7	0	theme	structural	1160:1169	arg1	characteristics					1171:1185	their structural characteristics	1154:1185	their structural characteristics	1154:1185	Their fermentability by prebiotic species can be ascribed more to their structural characteristics and monosaccharide composition than their molecular dimensions.					
34087714	2	1	theme	molar	369:373	arg1	polysaccharides					380:394	high molar mass polysaccharides	364:394	high molar mass polysaccharides	364:394	Two were composed of high molar mass polysaccharides, and one included water-soluble mono-, di-, and oligosaccharides.					
34087714	3	2	theme	galacturonic	647:658	arg1	acid					660:663	galacturonic acid	647:663	galacturonic acid	647:663	The large polysaccharides have a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1 and are consistently composed of galactose, arabinose, xylose, and rhamnose; however, the content of galacturonic acid was different between both fractions and species.					
34087714	2	3	theme	high	364:367	arg1	polysaccharides					380:394	high molar mass polysaccharides	364:394	high molar mass polysaccharides	364:394	Two were composed of high molar mass polysaccharides, and one included water-soluble mono-, di-, and oligosaccharides.					
34087714	3	4	theme	large	466:470	arg1	polysaccharides					472:486	The large polysaccharides	462:486	The large polysaccharides	462:486	The large polysaccharides have a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1 and are consistently composed of galactose, arabinose, xylose, and rhamnose; however, the content of galacturonic acid was different between both fractions and species.					
34087714	1	5	theme	economic	266:273	arg1	importance					275:284	economic importance	266:284	economic importance	266:284	The mucilage extracted from the convection-dried cladodes of O. ficus-indica and O. joconostle, two species of economic importance, delivered three fractions after methanol precipitation.					
34087714	1	6	theme	importance	275:284	arg1	mucilage					159:166	The mucilage	155:166	The mucilage extracted from the convection-dried cladodes of O. ficus-indica and O. joconostle	155:248	The mucilage extracted from the convection-dried cladodes of O. ficus-indica and O. joconostle, two species of economic importance, delivered three fractions after methanol precipitation.					
34087714	1	6	theme	importance	275:284	arg1	species					255:261	two species	251:261	two species of economic importance	251:284	The mucilage extracted from the convection-dried cladodes of O. ficus-indica and O. joconostle, two species of economic importance, delivered three fractions after methanol precipitation.					
34087714	3	7	theme	g·mol-1	538:544	arg1	range					506:510	a molar mass range	493:510	a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1	493:544	The large polysaccharides have a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1 and are consistently composed of galactose, arabinose, xylose, and rhamnose; however, the content of galacturonic acid was different between both fractions and species.					
34087714	6	8	theme	complex-structured	984:1001	arg1	galactooligosaccharides					1003:1025	simple- and complex-structured galactooligosaccharides	972:1025	simple- and complex-structured galactooligosaccharides with arabinosyl-, xylosyl- and galacturonosyl acid residues	972:1085	These include simple- and complex-structured galactooligosaccharides with arabinosyl-, xylosyl- and galacturonosyl acid residues.					
34087714	5	9	theme	third	891:895	arg1	fraction					897:904	the third fraction	887:904	the third fraction	887:904	In the third fraction, previously unreported oligosaccharides were found.					
34087714	4	10	theme	galacturonic	848:859	arg1	acid					861:864	galacturonic acid	848:864	galacturonic acid	848:864	Their fermentability by selected probiotics was relatively low, 11-27 % compared to glucose, and decreased with increasing levels of galacturonic acid in the molecules.					
34087714	4	11	theme	selected	739:746	arg1	probiotics					748:757	selected probiotics	739:757	selected probiotics	739:757	Their fermentability by selected probiotics was relatively low, 11-27 % compared to glucose, and decreased with increasing levels of galacturonic acid in the molecules.					
34087714	1	12	theme	convection-dried	187:202	arg1	cladodes					204:211	the convection-dried cladodes	183:211	the convection-dried cladodes of O. ficus-indica and O. joconostle	183:248	The mucilage extracted from the convection-dried cladodes of O. ficus-indica and O. joconostle, two species of economic importance, delivered three fractions after methanol precipitation.					
34087714	0	13	theme	Prebiotic	0:8	arg1	potential					10:18	Prebiotic potential	0:18	Prebiotic potential	0:18	Prebiotic potential and chemical characterization of the poly and oligosaccharides present in the mucilage of Opuntia ficus-indica and Opuntia joconostle.					
34087714	4	14	theme	acid	861:864	arg1	levels					838:843	levels	838:843	levels of galacturonic acid	838:864	Their fermentability by selected probiotics was relatively low, 11-27 % compared to glucose, and decreased with increasing levels of galacturonic acid in the molecules.					
34087714	0	15	theme	chemical	24:31	arg1	characterization					33:48	chemical characterization	24:48	chemical characterization	24:48	Prebiotic potential and chemical characterization of the poly and oligosaccharides present in the mucilage of Opuntia ficus-indica and Opuntia joconostle.					
34087714	6	16	theme	simple-	972:978	arg1	galactooligosaccharides					1003:1025	simple- and complex-structured galactooligosaccharides	972:1025	simple- and complex-structured galactooligosaccharides with arabinosyl-, xylosyl- and galacturonosyl acid residues	972:1085	These include simple- and complex-structured galactooligosaccharides with arabinosyl-, xylosyl- and galacturonosyl acid residues.					
34087714	7	17	theme	prebiotic	1112:1120	arg1	species					1122:1128	prebiotic species	1112:1128	prebiotic species	1112:1128	Their fermentability by prebiotic species can be ascribed more to their structural characteristics and monosaccharide composition than their molecular dimensions.					
34087714	5	18	theme	unreported	918:927	arg1	oligosaccharides					929:944	previously unreported oligosaccharides	907:944	previously unreported oligosaccharides	907:944	In the third fraction, previously unreported oligosaccharides were found.					
34087714	7	19	theme	molecular	1229:1237	arg1	dimensions					1239:1248	their molecular dimensions	1223:1248	their molecular dimensions	1223:1248	Their fermentability by prebiotic species can be ascribed more to their structural characteristics and monosaccharide composition than their molecular dimensions.					
34087714	0	20	theme	ficus-indica	118:129	arg1	mucilage					98:105	the mucilage	94:105	the mucilage of Opuntia ficus-indica and Opuntia joconostle	94:152	Prebiotic potential and chemical characterization of the poly and oligosaccharides present in the mucilage of Opuntia ficus-indica and Opuntia joconostle.					
34087714	3	21	dep	8.0 × 105	528:536	arg1	to					525:526	to	525:526	to	525:526	The large polysaccharides have a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1 and are consistently composed of galactose, arabinose, xylose, and rhamnose; however, the content of galacturonic acid was different between both fractions and species.					
34087714	1	22	theme	O.	216:217	arg1	cladodes					204:211	the convection-dried cladodes	183:211	the convection-dried cladodes of O. ficus-indica and O. joconostle	183:248	The mucilage extracted from the convection-dried cladodes of O. ficus-indica and O. joconostle, two species of economic importance, delivered three fractions after methanol precipitation.					
34087714	0	23	theme	joconostle	143:152	arg1	mucilage					98:105	the mucilage	94:105	the mucilage of Opuntia ficus-indica and Opuntia joconostle	94:152	Prebiotic potential and chemical characterization of the poly and oligosaccharides present in the mucilage of Opuntia ficus-indica and Opuntia joconostle.					
34087714	0	24	from	present	83:89	arg1	mucilage					98:105	the mucilage	94:105	the mucilage of Opuntia ficus-indica and Opuntia joconostle	94:152	Prebiotic potential and chemical characterization of the poly and oligosaccharides present in the mucilage of Opuntia ficus-indica and Opuntia joconostle.					
34087714	6	25	theme	galacturonosyl	1058:1071	arg1	residues					1078:1085	galacturonosyl acid residues	1058:1085	galacturonosyl acid residues	1058:1085	These include simple- and complex-structured galactooligosaccharides with arabinosyl-, xylosyl- and galacturonosyl acid residues.					
34087714	3	26	contain	have	488:491	arg2	range					506:510	a molar mass range	493:510	a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1	493:544	The large polysaccharides have a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1 and are consistently composed of galactose, arabinose, xylose, and rhamnose; however, the content of galacturonic acid was different between both fractions and species.					
34087714	3	26	contain	have	488:491	arg1	polysaccharides					472:486	The large polysaccharides	462:486	The large polysaccharides	462:486	The large polysaccharides have a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1 and are consistently composed of galactose, arabinose, xylose, and rhamnose; however, the content of galacturonic acid was different between both fractions and species.					
34087714	2	27	theme	water-soluble	414:426	arg1	mono-					428:432	water-soluble mono-	414:432	water-soluble mono-	414:432	Two were composed of high molar mass polysaccharides, and one included water-soluble mono-, di-, and oligosaccharides.					
34087714	0	28	theme	poly	57:60	arg1	potential					10:18	Prebiotic potential	0:18	Prebiotic potential	0:18	Prebiotic potential and chemical characterization of the poly and oligosaccharides present in the mucilage of Opuntia ficus-indica and Opuntia joconostle.					
34087714	0	28	theme	poly	57:60	arg1	characterization					33:48	chemical characterization	24:48	chemical characterization	24:48	Prebiotic potential and chemical characterization of the poly and oligosaccharides present in the mucilage of Opuntia ficus-indica and Opuntia joconostle.					
34087714	1	29	theme	methanol	319:326	arg1	precipitation					328:340	methanol precipitation	319:340	methanol precipitation	319:340	The mucilage extracted from the convection-dried cladodes of O. ficus-indica and O. joconostle, two species of economic importance, delivered three fractions after methanol precipitation.					
34087714	6	30	theme	acid	1073:1076	arg1	residues					1078:1085	galacturonosyl acid residues	1058:1085	galacturonosyl acid residues	1058:1085	These include simple- and complex-structured galactooligosaccharides with arabinosyl-, xylosyl- and galacturonosyl acid residues.					
34087714	3	31	theme	mass	501:504	arg1	range					506:510	a molar mass range	493:510	a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1	493:544	The large polysaccharides have a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1 and are consistently composed of galactose, arabinose, xylose, and rhamnose; however, the content of galacturonic acid was different between both fractions and species.					
34087714	7	32	theme	monosaccharide	1191:1204	arg1	composition					1206:1216	monosaccharide composition	1191:1216	monosaccharide composition	1191:1216	Their fermentability by prebiotic species can be ascribed more to their structural characteristics and monosaccharide composition than their molecular dimensions.					
34087714	5	33	located	found	951:955	arg2	oligosaccharides					929:944	previously unreported oligosaccharides	907:944	previously unreported oligosaccharides	907:944	In the third fraction, previously unreported oligosaccharides were found.					
34087714	5	33	located	found	951:955	arg1	fraction					897:904	the third fraction	887:904	the third fraction	887:904	In the third fraction, previously unreported oligosaccharides were found.					
34087714	6	34	with	galactooligosaccharides	1003:1025	arg1	xylosyl-					1045:1052	xylosyl-	1045:1052	xylosyl-	1045:1052	These include simple- and complex-structured galactooligosaccharides with arabinosyl-, xylosyl- and galacturonosyl acid residues.					
34087714	6	34	with	galactooligosaccharides	1003:1025	arg1	arabinosyl-					1032:1042	arabinosyl-	1032:1042	arabinosyl-	1032:1042	These include simple- and complex-structured galactooligosaccharides with arabinosyl-, xylosyl- and galacturonosyl acid residues.					
34087714	6	34	with	galactooligosaccharides	1003:1025	arg1	residues					1078:1085	galacturonosyl acid residues	1058:1085	galacturonosyl acid residues	1058:1085	These include simple- and complex-structured galactooligosaccharides with arabinosyl-, xylosyl- and galacturonosyl acid residues.					
34087714	1	35	theme	O.	236:237	arg1	cladodes					204:211	the convection-dried cladodes	183:211	the convection-dried cladodes of O. ficus-indica and O. joconostle	183:248	The mucilage extracted from the convection-dried cladodes of O. ficus-indica and O. joconostle, two species of economic importance, delivered three fractions after methanol precipitation.					
34087714	0	36	from	mucilage	98:105	arg1	present					83:89	present	83:89	present	83:89	Prebiotic potential and chemical characterization of the poly and oligosaccharides present in the mucilage of Opuntia ficus-indica and Opuntia joconostle.					
34087714	3	37	theme	molar	495:499	arg1	range					506:510	a molar mass range	493:510	a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1	493:544	The large polysaccharides have a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1 and are consistently composed of galactose, arabinose, xylose, and rhamnose; however, the content of galacturonic acid was different between both fractions and species.					
34087714	3	38	theme	acid	660:663	arg1	content					636:642	the content	632:642	the content of galacturonic acid	632:663	The large polysaccharides have a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1 and are consistently composed of galactose, arabinose, xylose, and rhamnose; however, the content of galacturonic acid was different between both fractions and species.					
34087714	3	38	theme	acid	660:663	arg1	different					669:677	different	669:677	different	669:677	The large polysaccharides have a molar mass range of 4.0 × 103 to 8.0 × 105 g·mol-1 and are consistently composed of galactose, arabinose, xylose, and rhamnose; however, the content of galacturonic acid was different between both fractions and species.					
34087714	1	39	dep	O.	216:217	arg1	ficus-indica					219:230	O. ficus-indica	216:230	O. ficus-indica	216:230	The mucilage extracted from the convection-dried cladodes of O. ficus-indica and O. joconostle, two species of economic importance, delivered three fractions after methanol precipitation.					
34087714	1	39	dep	O.	216:217	arg1	joconostle					239:248	joconostle	239:248	joconostle	239:248	The mucilage extracted from the convection-dried cladodes of O. ficus-indica and O. joconostle, two species of economic importance, delivered three fractions after methanol precipitation.					
34087714	2	40	theme	mass	375:378	arg1	polysaccharides					380:394	high molar mass polysaccharides	364:394	high molar mass polysaccharides	364:394	Two were composed of high molar mass polysaccharides, and one included water-soluble mono-, di-, and oligosaccharides.					
33010272	7	0	theme	HABSF	1453:1457	arg1	effects					1432:1438	the different effects	1418:1438	the different effects of HHBSF and HABSF	1418:1457	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	1	1	theme	Handroanthus	166:177	arg1	trees					147:151	trees	147:151	trees of the genus Handroanthus	147:177	Barks of trees of the genus Handroanthus are known for their antitumor activity, which is attributed to naphthoquinones.					
33010272	6	2	theme	only	1155:1158	arg1	HABSF					1160:1164	only HABSF	1155:1164	only HABSF	1155:1164	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	0	3	theme	Structural	86:95	arg1	characterization					97:112	Structural characterization	86:112	Structural characterization	86:112	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	0	3	theme	Structural	86:95	arg1	fractions					15:23	Polysaccharide fractions	0:23	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.	0:136	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	7	4	theme	different	1422:1430	arg1	effects					1432:1438	the different effects	1418:1438	the different effects of HHBSF and HABSF	1418:1457	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	5	5	from	effects	917:923	arg1	proliferation					928:940	proliferation	928:940	proliferation of Caco-2 and MCF-7 cells	928:966	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	2	6	theme	Handroanthus	364:375	arg1	barks					377:381	Handroanthus barks	364:381	Handroanthus barks	364:381	Another class of molecules that has shown antitumor activity are the polysaccharides, however those from Handroanthus barks have never been studied.					
33010272	0	7	theme	cytotoxic	118:126	arg1	activity					128:135	cytotoxic activity	118:135	cytotoxic activity	118:135	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	0	7	theme	cytotoxic	118:126	arg1	fractions					15:23	Polysaccharide fractions	0:23	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.	0:136	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	6	8	theme	MCF-7	1204:1208	arg1	growth					1194:1199	the growth	1190:1199	the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells	1190:1264	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	2	9	theme	antitumor	301:309	arg1	activity					311:318	antitumor activity	301:318	antitumor activity	301:318	Another class of molecules that has shown antitumor activity are the polysaccharides, however those from Handroanthus barks have never been studied.					
33010272	5	10	theme	MCF-7	956:960	arg1	cells					962:966	Caco-2 and MCF-7 cells	945:966	cells	962:966	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	0	11	from	albus	73:77	arg1	characterization					97:112	Structural characterization	86:112	Structural characterization	86:112	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	0	11	from	albus	73:77	arg1	activity					128:135	cytotoxic activity	118:135	cytotoxic activity	118:135	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	0	11	from	albus	73:77	arg1	fractions					15:23	Polysaccharide fractions	0:23	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.	0:136	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	7	12	theme	HHBSF	1443:1447	arg1	effects					1432:1438	the different effects	1418:1438	the different effects of HHBSF and HABSF	1418:1457	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	5	13	theme	MTT	989:991	arg1	assay					1008:1012	MTT cell viability assay	989:1012	MTT cell viability assay	989:1012	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	5	14	theme	soluble	778:784	arg1	HHBSF					811:815	HHBSF	811:815	HHBSF	811:815	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	5	14	theme	soluble	778:784	arg1	fractions					801:809	The soluble polysaccharide fractions	774:809	The soluble polysaccharide fractions HHBSF and HABSF	774:825	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	5	14	theme	soluble	778:784	arg1	HABSF					821:825	HABSF	821:825	HABSF	821:825	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	6	15	theme	cells	1260:1264	arg1	growth					1194:1199	the growth	1190:1199	the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells	1190:1264	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	3	16	dep	H.	499:500	arg1	albus					502:506	H. albus	499:506	H. albus	499:506	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	5	17	theme	Caco-2	945:950	arg1	cells					962:966	Caco-2 and MCF-7 cells	945:966	cells	962:966	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	7	18	theme	polysaccharides	1325:1339	arg1	proportion					1305:1314	proportion	1305:1314	proportion	1305:1314	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	7	18	theme	polysaccharides	1325:1339	arg1	structure					1291:1299	fine structure	1286:1299	fine structure	1286:1299	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	5	19	theme	viability	998:1006	arg1	assay					1008:1012	MTT cell viability assay	989:1012	MTT cell viability assay	989:1012	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	5	20	theme	NMR	893:895	arg1	analyses					897:904	NMR analyses	893:904	NMR analyses	893:904	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	7	21	theme	associated	1374:1383	arg1	compounds					1394:1402	associated phenolic compounds	1374:1402	associated phenolic compounds	1374:1402	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	0	22	from	heptaphyllus	43:54	arg1	characterization					97:112	Structural characterization	86:112	Structural characterization	86:112	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	0	22	from	heptaphyllus	43:54	arg1	activity					128:135	cytotoxic activity	118:135	cytotoxic activity	118:135	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	0	22	from	heptaphyllus	43:54	arg1	fractions					15:23	Polysaccharide fractions	0:23	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.	0:136	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	7	23	theme	fine	1286:1289	arg1	structure					1291:1299	fine structure	1286:1299	fine structure	1286:1299	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	7	24	from	Differences	1267:1277	arg1	proportion					1305:1314	proportion	1305:1314	proportion	1305:1314	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	7	24	from	Differences	1267:1277	arg1	structure					1291:1299	fine structure	1286:1299	fine structure	1286:1299	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	7	24	from	Differences	1267:1277	arg1	composition					1359:1369	the composition	1355:1369	the composition of associated phenolic compounds	1355:1402	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	3	25	theme	cytotoxic	571:579	arg1	effects					581:587	their cytotoxic effects	565:587	their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively	565:673	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	6	26	theme	arabinogalactan	1086:1100	arg1	RGI					1140:1142	RGI	1140:1142	RGI	1140:1142	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	6	26	theme	arabinogalactan	1086:1100	arg1	rhamnogalacturonan					1120:1137	galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan	1058:1137	galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI)	1058:1143	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	0	27	theme	Polysaccharide	0:13	arg1	characterization					97:112	Structural characterization	86:112	Structural characterization	86:112	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	0	27	theme	Polysaccharide	0:13	arg1	activity					128:135	cytotoxic activity	118:135	cytotoxic activity	118:135	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	0	27	theme	Polysaccharide	0:13	arg1	fractions					15:23	Polysaccharide fractions	0:23	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.	0:136	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	5	28	dep	fractions	801:809	arg1	HHBSF					811:815	HHBSF	811:815	HHBSF	811:815	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	5	28	dep	fractions	801:809	arg1	fractions					801:809	The soluble polysaccharide fractions	774:809	The soluble polysaccharide fractions HHBSF and HABSF	774:825	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	5	28	dep	fractions	801:809	arg1	HABSF					821:825	HABSF	821:825	HABSF	821:825	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	3	29	from	H.	499:500	arg1	polysaccharides					458:472	extract polysaccharides	450:472	extract polysaccharides from H. heptaphyllus and H. albus barks	450:512	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	29	from	H.	499:500	arg1	aim					425:427	the aim	421:427	the aim of this study	421:441	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	5	30	theme	cells	962:966	arg1	proliferation					928:940	proliferation	928:940	proliferation of Caco-2 and MCF-7 cells	928:966	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	3	31	theme	study	437:441	arg1	polysaccharides					458:472	extract polysaccharides	450:472	extract polysaccharides from H. heptaphyllus and H. albus barks	450:512	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	31	theme	study	437:441	arg1	aim					425:427	the aim	421:427	the aim of this study	421:441	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	7	32	theme	maybe	1346:1350	arg1	proportion					1305:1314	proportion	1305:1314	proportion	1305:1314	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	7	32	theme	maybe	1346:1350	arg1	structure					1291:1299	fine structure	1286:1299	fine structure	1286:1299	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	7	33	theme	compounds	1394:1402	arg1	composition					1359:1369	the composition	1355:1369	the composition of associated phenolic compounds	1355:1402	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	6	34	theme	Caco-2	1233:1238	arg1	cells					1260:1264	MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells	1204:1264	cells	1260:1264	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	6	34	theme	Caco-2	1233:1238	arg1	CC50 = 2258 μg/mL					1241:1257	CC50 = 2258 μg/mL	1241:1257	CC50 = 2258 μg/mL	1241:1257	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	5	35	theme	monosaccharide	849:862	arg1	composition					864:874	monosaccharide composition	849:874	monosaccharide composition	849:874	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	5	36	theme	cell	993:996	arg1	assay					1008:1012	MTT cell viability assay	989:1012	MTT cell viability assay	989:1012	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	5	37	theme	polysaccharide	786:799	arg1	HHBSF					811:815	HHBSF	811:815	HHBSF	811:815	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	5	37	theme	polysaccharide	786:799	arg1	fractions					801:809	The soluble polysaccharide fractions	774:809	The soluble polysaccharide fractions HHBSF and HABSF	774:825	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	5	37	theme	polysaccharide	786:799	arg1	HABSF					821:825	HABSF	821:825	HABSF	821:825	The soluble polysaccharide fractions HHBSF and HABSF were characterized by monosaccharide composition, methylation and NMR analyses, and their effects on proliferation of Caco-2 and MCF-7 cells were evaluated using MTT cell viability assay.					
33010272	6	38	theme	galactoglucomannan	1058:1075	arg1	RGI					1140:1142	RGI	1140:1142	RGI	1140:1142	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	6	38	theme	galactoglucomannan	1058:1075	arg1	rhamnogalacturonan					1120:1137	galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan	1058:1137	galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI)	1058:1143	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	3	39	theme	human	596:600	arg1	MCF-7					655:659	MCF-7	655:659	MCF-7	655:659	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	39	theme	human	596:600	arg1	colon					602:606	the human colon and human breast cancer cell lines	592:641	colon	602:606	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	39	theme	human	596:600	arg1	Caco-2					644:649	Caco-2	644:649	Caco-2	644:649	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	1	40	theme	antitumor	199:207	arg1	activity					209:216	their antitumor activity	193:216	their antitumor activity	193:216	Barks of trees of the genus Handroanthus are known for their antitumor activity, which is attributed to naphthoquinones.					
33010272	3	41	theme	extract	450:456	arg1	polysaccharides					458:472	extract polysaccharides	450:472	extract polysaccharides from H. heptaphyllus and H. albus barks	450:512	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	41	theme	extract	450:456	arg1	aim					425:427	the aim	421:427	the aim of this study	421:441	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	42	theme	cell	632:635	arg1	lines					637:641	the human colon and human breast cancer cell lines	592:641	lines	637:641	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	7	43	theme	phenolic	1385:1392	arg1	compounds					1394:1402	associated phenolic compounds	1374:1402	associated phenolic compounds	1374:1402	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	7	44	dep	structure	1291:1299	arg1	the					1282:1284	the	1282:1284	the	1282:1284	Differences in the fine structure and proportion of their polysaccharides, and maybe in the composition of associated phenolic compounds could explain the different effects of HHBSF and HABSF.					
33010272	0	45	dep	heptaphyllus	43:54	arg1	barks					79:83	barks	79:83	barks	79:83	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	3	46	dep	H.	479:480	arg1	heptaphyllus					482:493	H. heptaphyllus	479:493	H. heptaphyllus	479:493	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	46	dep	H.	479:480	arg1	barks					508:512	barks	508:512	barks	508:512	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	2	47	theme	molecules	276:284	arg1	class					267:271	Another class	259:271	Another class of molecules that has shown antitumor activity	259:318	Another class of molecules that has shown antitumor activity are the polysaccharides, however those from Handroanthus barks have never been studied.					
33010272	2	47	theme	molecules	276:284	arg1	polysaccharides					328:342	the polysaccharides	324:342	the polysaccharides	324:342	Another class of molecules that has shown antitumor activity are the polysaccharides, however those from Handroanthus barks have never been studied.					
33010272	3	48	theme	breast	618:623	arg1	lines					637:641	the human colon and human breast cancer cell lines	592:641	lines	637:641	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	49	from	H.	479:480	arg1	polysaccharides					458:472	extract polysaccharides	450:472	extract polysaccharides from H. heptaphyllus and H. albus barks	450:512	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	49	from	H.	479:480	arg1	aim					425:427	the aim	421:427	the aim of this study	421:441	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	1	50	theme	trees	147:151	arg1	Barks					138:142	Barks	138:142	Barks of trees of the genus Handroanthus	138:177	Barks of trees of the genus Handroanthus are known for their antitumor activity, which is attributed to naphthoquinones.					
33010272	3	51	theme	cancer	625:630	arg1	lines					637:641	the human colon and human breast cancer cell lines	592:641	lines	637:641	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	6	52	theme	type	1113:1116	arg1	RGI					1140:1142	RGI	1140:1142	RGI	1140:1142	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	6	52	theme	type	1113:1116	arg1	rhamnogalacturonan					1120:1137	galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan	1058:1137	galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI)	1058:1143	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	2	53	dep	polysaccharides	328:342	arg1	studied					399:405	studied	399:405	have never been studied	383:405	Another class of molecules that has shown antitumor activity are the polysaccharides, however those from Handroanthus barks have never been studied.					
33010272	0	54	dep	fractions	15:23	arg1	characterization					97:112	Structural characterization	86:112	Structural characterization	86:112	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	0	54	dep	fractions	15:23	arg1	activity					128:135	cytotoxic activity	118:135	cytotoxic activity	118:135	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	0	54	dep	fractions	15:23	arg1	fractions					15:23	Polysaccharide fractions	0:23	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.	0:136	Polysaccharide fractions from Handroanthus heptaphyllus and Handroanthus albus barks: Structural characterization and cytotoxic activity.					
33010272	6	55	theme	type	1078:1081	arg1	arabinogalactan					1086:1100	type II arabinogalactan	1078:1100	type II arabinogalactan (AGII)	1078:1107	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	6	55	theme	type	1078:1081	arg1	AGII					1103:1106	AGII	1103:1106	AGII	1103:1106	HHBSF and HABSF were mainly constituted of galactoglucomannan, type II arabinogalactan (AGII) and type I rhamnogalacturonan (RGI), however, only HABSF significantly inhibited the growth of MCF-7 (CC50 = 327 μg/mL) and Caco-2 (CC50 = 2258 μg/mL) cells.					
33010272	3	56	from	effects	581:587	arg1	MCF-7					655:659	MCF-7	655:659	MCF-7	655:659	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	56	from	effects	581:587	arg1	Caco-2					644:649	Caco-2	644:649	Caco-2	644:649	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	56	from	effects	581:587	arg1	colon					602:606	the human colon and human breast cancer cell lines	592:641	colon	602:606	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	56	from	effects	581:587	arg1	lines					637:641	the human colon and human breast cancer cell lines	592:641	lines	637:641	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	3	57	theme	human	612:616	arg1	lines					637:641	the human colon and human breast cancer cell lines	592:641	lines	637:641	Accordingly, the aim of this study was to extract polysaccharides from H. heptaphyllus and H. albus barks, to characterize them structurally and to evaluate their cytotoxic effects on the human colon and human breast cancer cell lines, Caco-2 and MCF-7, respectively.					
33010272	1	58	theme	genus	160:164	arg1	Handroanthus					166:177	the genus Handroanthus	156:177	the genus Handroanthus	156:177	Barks of trees of the genus Handroanthus are known for their antitumor activity, which is attributed to naphthoquinones.					
33462808	2	0	theme	%	574:574	arg1	yield					552:556	an yield	549:556	an yield of 6.137 ± 0.062%	549:574	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	6	1	theme	natural	1123:1129	arg1	resource					1131:1138	potential natural resource	1113:1138	potential natural resource of antioxidants for medicine and functional foods	1113:1188	The results suggested that PSMP is potential natural resource of antioxidants for medicine and functional foods.					
33462808	3	2	theme	glycosidic	664:673	arg1	linkages					675:682	glycosidic linkages	664:682	glycosidic linkages	664:682	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	4	3	theme	microscopy	906:915	arg1	analysis					917:924	Scanning electron microscopy analysis	888:924	Scanning electron microscopy analysis	888:924	Scanning electron microscopy analysis showed that many holes were formed on the surface of PSM after UAE.					
33462808	3	4	theme	exclusion	708:716	arg1	chromatography					718:731	size exclusion chromatography	703:731	size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	703:885	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	9	5	theme	other	1503:1507	arg1	resources					1532:1540	other medicine-food dual-use resources	1503:1540	other medicine-food dual-use resources	1503:1540	This study provides effective reference for in-depth research on other medicine-food dual-use resources.					
33462808	4	6	theme	Scanning	888:895	arg1	microscopy					906:915	Scanning electron microscopy	888:915	Scanning electron microscopy analysis	888:924	Scanning electron microscopy analysis showed that many holes were formed on the surface of PSM after UAE.					
33462808	3	7	theme	light	749:753	arg1	scattering					755:764	multiangle light scattering	738:764	multiangle light scattering	738:764	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	1	8	theme	surface	200:206	arg1	methodology					208:218	response surface methodology	191:218	response surface methodology	191:218	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	11	9	theme	raw	1979:1981	arg1	utilization					1992:2002	raw material utilization	1979:2002	raw material utilization	1979:2002	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	10	10	theme	efficiency	1695:1704	arg1	advantages					1676:1685	advantages	1676:1685	advantages of high efficiency and energy saving	1676:1722	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	3	11	theme	chromatography-mass	771:789	arg1	spectrometry					791:802	gas chromatography-mass spectrometry	767:802	gas chromatography-mass spectrometry	767:802	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	4	12	theme	many	938:941	arg1	holes					943:947	many holes	938:947	many holes	938:947	Scanning electron microscopy analysis showed that many holes were formed on the surface of PSM after UAE.					
33462808	8	13	theme	consumer	1334:1341	arg1	demands					1343:1349	consumer demands	1334:1349	consumer demands for newly developed foods with natural	1334:1388	With consumer demands for newly developed foods with natural, wholesome ingredients are increasing nowadays.					
33462808	11	14	theme	production	1885:1894	arg1	cost					1877:1880	total cost	1871:1880	total cost of production	1871:1894	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	3	15	theme	infrared	822:829	arg1	spectroscopy					831:842	Fourier-transfer infrared spectroscopy	805:842	Fourier-transfer infrared spectroscopy	805:842	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	3	16	theme	resonance	866:874	arg1	detections					876:885	multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	738:885	multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	738:885	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	2	17	theme	ultrasonic	488:497	arg1	power					499:503	ultrasonic power	488:503	ultrasonic power of 229.00 W	488:515	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	8	18	theme	developed	1361:1369	arg1	foods					1371:1375	newly developed foods	1355:1375	newly developed foods with natural	1355:1388	With consumer demands for newly developed foods with natural, wholesome ingredients are increasing nowadays.					
33462808	7	19	dep	APPLICATION	1201:1211	arg1	selection					1218:1226	The selection	1214:1226	The selection of raw material perilla seed meal	1214:1260	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	7	19	dep	APPLICATION	1201:1211	arg1	conducive					1265:1273	conducive	1265:1273	conducive	1265:1273	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	3	20	theme	nuclear	849:855	arg1	resonance					866:874	nuclear magnetic resonance	849:874	nuclear magnetic resonance	849:874	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	7	21	theme	meal	1257:1260	arg1	conducive					1265:1273	conducive	1265:1273	conducive	1265:1273	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	7	21	theme	meal	1257:1260	arg1	selection					1218:1226	The selection	1214:1226	The selection of raw material perilla seed meal	1214:1260	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	2	22	theme	W	515:515	arg1	temperature					429:439	ultrasonic temperature	418:439	ultrasonic temperature of 43.00 °C	418:451	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	22	theme	W	515:515	arg1	ratio					397:401	liquid-solid ratio	384:401	liquid-solid ratio of 26.00 mL/g	384:415	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	22	theme	W	515:515	arg1	conditions					530:539	the optimal conditions	518:539	the optimal conditions	518:539	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	22	theme	W	515:515	arg1	conditions					351:360	The optimal conditions	339:360	The optimal conditions for UAE of PSMP were	339:381	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	22	theme	W	515:515	arg1	time					465:468	ultrasonic time	454:468	ultrasonic time of 52.00 min	454:481	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	22	theme	W	515:515	arg1	power					499:503	ultrasonic power	488:503	ultrasonic power of 229.00 W	488:515	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	0	23	theme	surface	101:107	arg1	methodology					109:119	response surface methodology	92:119	response surface methodology	92:119	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	11	24	theme	material	1983:1990	arg1	utilization					1992:2002	raw material utilization	1979:2002	raw material utilization	1979:2002	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	7	25	theme	raw	1231:1233	arg1	meal					1257:1260	raw material perilla seed meal	1231:1260	raw material perilla seed meal	1231:1260	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	11	26	theme	industrial	1818:1827	arg1	application					1829:1839	industrial application	1818:1839	industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization	1818:2002	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	2	27	theme	°C	450:451	arg1	temperature					429:439	ultrasonic temperature	418:439	ultrasonic temperature of 43.00 °C	418:451	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	27	theme	°C	450:451	arg1	ratio					397:401	liquid-solid ratio	384:401	liquid-solid ratio of 26.00 mL/g	384:415	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	27	theme	°C	450:451	arg1	conditions					530:539	the optimal conditions	518:539	the optimal conditions	518:539	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	27	theme	°C	450:451	arg1	conditions					351:360	The optimal conditions	339:360	The optimal conditions for UAE of PSMP were	339:381	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	27	theme	°C	450:451	arg1	time					465:468	ultrasonic time	454:468	ultrasonic time of 52.00 min	454:481	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	27	theme	°C	450:451	arg1	power					499:503	ultrasonic power	488:503	ultrasonic power of 229.00 W	488:515	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	10	28	theme	saving	1717:1722	arg1	advantages					1676:1685	advantages	1676:1685	advantages of high efficiency and energy saving	1676:1722	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	2	29	theme	ultrasonic	418:427	arg1	temperature					429:439	ultrasonic temperature	418:439	ultrasonic temperature of 43.00 °C	418:451	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	0	30	theme	ultrasound-assisted	16:34	arg1	extraction					36:45	ultrasound-assisted extraction	16:45	ultrasound-assisted extraction of polysaccharides from perilla	16:77	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	1	31	theme	seed	305:308	arg1	PSMP					332:335	PSMP	332:335	PSMP	332:335	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	1	31	theme	seed	305:308	arg1	polysaccharides					315:329	perilla seed meal polysaccharides	297:329	perilla seed meal polysaccharides (PSMP)	297:336	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	9	32	from	research	1491:1498	arg1	resources					1532:1540	other medicine-food dual-use resources	1503:1540	other medicine-food dual-use resources	1503:1540	This study provides effective reference for in-depth research on other medicine-food dual-use resources.					
33462808	0	33	dep	seed	79:82	arg1	activities					164:173	in vitro antioxidant activities	143:173	in vitro antioxidant activities	143:173	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	0	33	dep	seed	79:82	arg1	Characterization					122:137	Characterization	122:137	Characterization	122:137	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	2	34	theme	26.00 mL/g	406:415	arg1	temperature					429:439	ultrasonic temperature	418:439	ultrasonic temperature of 43.00 °C	418:451	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	34	theme	26.00 mL/g	406:415	arg1	ratio					397:401	liquid-solid ratio	384:401	liquid-solid ratio of 26.00 mL/g	384:415	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	34	theme	26.00 mL/g	406:415	arg1	conditions					530:539	the optimal conditions	518:539	the optimal conditions	518:539	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	34	theme	26.00 mL/g	406:415	arg1	conditions					351:360	The optimal conditions	339:360	The optimal conditions for UAE of PSMP were	339:381	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	34	theme	26.00 mL/g	406:415	arg1	time					465:468	ultrasonic time	454:468	ultrasonic time of 52.00 min	454:481	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	34	theme	26.00 mL/g	406:415	arg1	power					499:503	ultrasonic power	488:503	ultrasonic power of 229.00 W	488:515	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	7	35	theme	PRACTICAL	1191:1199	arg1	APPLICATION					1201:1211	PRACTICAL APPLICATION	1191:1211	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.	1191:1327	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	1	36	theme	polysaccharides	315:329	arg1	process					286:292	the ultrasound-assisted extraction (UAE) process	245:292	the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP)	245:336	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	5	37	theme	various	1053:1059	arg1	assays					1061:1066	various assays	1053:1066	various assays	1053:1066	The antioxidant activities of PSMP were investigated using various assays in vitro.					
33462808	2	38	theme	ultrasonic	454:463	arg1	time					465:468	ultrasonic time	454:468	ultrasonic time of 52.00 min	454:481	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	3	39	theme	molecular	611:619	arg1	weight					621:626	molecular weight	611:626	molecular weight	611:626	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	10	40	theme	promising	1585:1593	arg1	extraction					1563:1572	Ultrasound-assisted extraction	1543:1572	Ultrasound-assisted extraction (UAE)	1543:1578	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	10	40	theme	promising	1585:1593	arg1	method					1607:1612	a promising alternative method	1583:1612	a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving	1583:1722	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	8	41	with	foods	1371:1375	arg1	natural					1382:1388	natural	1382:1388	natural	1382:1388	With consumer demands for newly developed foods with natural, wholesome ingredients are increasing nowadays.					
33462808	11	42	theme	extraction	1912:1921	arg1	temperature					1923:1933	the extraction temperature	1908:1933	the extraction temperature	1908:1933	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	5	43	theme	PSMP	1024:1027	arg1	activities					1010:1019	The antioxidant activities	994:1019	The antioxidant activities of PSMP	994:1027	The antioxidant activities of PSMP were investigated using various assays in vitro.					
33462808	7	44	theme	edible	1311:1316	arg1	resources					1318:1326	edible resources	1311:1326	edible resources	1311:1326	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	10	45	theme	reflux	1628:1633	arg1	HWRE					1647:1650	HWRE	1647:1650	HWRE	1647:1650	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	10	45	theme	reflux	1628:1633	arg1	extraction					1635:1644	hot water reflux extraction	1618:1644	hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving	1618:1722	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	3	46	theme	monosaccharides	643:657	arg1	characteristics					592:606	The structural characteristics	577:606	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages	577:682	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	2	47	theme	PSMP	373:376	arg1	UAE					366:368	UAE	366:368	UAE of PSMP	366:376	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	3	48	with	chromatography	718:731	arg1	detections					876:885	multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	738:885	multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	738:885	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	1	49	theme	ultrasound-assisted	249:267	arg1	UAE					281:283	UAE	281:283	UAE	281:283	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	1	49	theme	ultrasound-assisted	249:267	arg1	extraction					269:278	ultrasound-assisted extraction	249:278	the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP)	245:336	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	3	50	theme	Fourier-transfer	805:820	arg1	spectroscopy					831:842	Fourier-transfer infrared spectroscopy	805:842	Fourier-transfer infrared spectroscopy	805:842	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	10	51	theme	hot	1618:1620	arg1	HWRE					1647:1650	HWRE	1647:1650	HWRE	1647:1650	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	10	51	theme	hot	1618:1620	arg1	extraction					1635:1644	hot water reflux extraction	1618:1644	hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving	1618:1722	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	7	52	theme	seed	1252:1255	arg1	meal					1257:1260	raw material perilla seed meal	1231:1260	raw material perilla seed meal	1231:1260	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	11	53	theme	extraction	1947:1956	arg1	time					1958:1961	extraction time	1947:1961	extraction time	1947:1961	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	1	54	theme	extraction	269:278	arg1	process					286:292	the ultrasound-assisted extraction (UAE) process	245:292	the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP)	245:336	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	4	55	theme	electron	897:904	arg1	microscopy					906:915	Scanning electron microscopy	888:915	Scanning electron microscopy analysis	888:924	Scanning electron microscopy analysis showed that many holes were formed on the surface of PSM after UAE.					
33462808	6	56	theme	potential	1113:1121	arg1	resource					1131:1138	potential natural resource	1113:1138	potential natural resource of antioxidants for medicine and functional foods	1113:1188	The results suggested that PSMP is potential natural resource of antioxidants for medicine and functional foods.					
33462808	3	57	theme	linkages	675:682	arg1	characteristics					592:606	The structural characteristics	577:606	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages	577:682	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	9	58	theme	in-depth	1482:1489	arg1	research					1491:1498	in-depth research	1482:1498	in-depth research on other medicine-food dual-use resources	1482:1540	This study provides effective reference for in-depth research on other medicine-food dual-use resources.					
33462808	1	59	theme	response	191:198	arg1	methodology					208:218	response surface methodology	191:218	response surface methodology	191:218	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	3	60	theme	size	703:706	arg1	chromatography					718:731	size exclusion chromatography	703:731	size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	703:885	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	9	61	theme	medicine-food	1509:1521	arg1	resources					1532:1540	other medicine-food dual-use resources	1503:1540	other medicine-food dual-use resources	1503:1540	This study provides effective reference for in-depth research on other medicine-food dual-use resources.					
33462808	10	62	theme	polysaccharides	1656:1670	arg1	HWRE					1647:1650	HWRE	1647:1650	HWRE	1647:1650	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	10	62	theme	polysaccharides	1656:1670	arg1	extraction					1635:1644	hot water reflux extraction	1618:1644	hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving	1618:1722	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	3	63	theme	scattering	755:764	arg1	detections					876:885	multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	738:885	multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	738:885	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	0	64	theme	in	143:144	arg1	activities					164:173	in vitro antioxidant activities	143:173	in vitro antioxidant activities	143:173	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	2	65	theme	optimal	522:528	arg1	conditions					530:539	the optimal conditions	518:539	the optimal conditions	518:539	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	6	66	theme	functional	1173:1182	arg1	foods					1184:1188	functional foods	1173:1188	functional foods	1173:1188	The results suggested that PSMP is potential natural resource of antioxidants for medicine and functional foods.					
33462808	3	67	theme	multiangle	738:747	arg1	scattering					755:764	multiangle light scattering	738:764	multiangle light scattering	738:764	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	0	68	theme	antioxidant	152:162	arg1	activities					164:173	in vitro antioxidant activities	143:173	in vitro antioxidant activities	143:173	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	9	69	theme	dual-use	1523:1530	arg1	resources					1532:1540	other medicine-food dual-use resources	1503:1540	other medicine-food dual-use resources	1503:1540	This study provides effective reference for in-depth research on other medicine-food dual-use resources.					
33462808	10	70	theme	high	1690:1693	arg1	efficiency					1695:1704	high efficiency	1690:1704	high efficiency	1690:1704	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	4	71	theme	PSM	979:981	arg1	surface					968:974	the surface	964:974	the surface of PSM after UAE	964:991	Scanning electron microscopy analysis showed that many holes were formed on the surface of PSM after UAE.					
33462808	11	72	from	suitable	1805:1812	arg1	work					1733:1736	this work	1728:1736	this work	1728:1736	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	3	73	theme	gas	767:769	arg1	spectrometry					791:802	gas chromatography-mass spectrometry	767:802	gas chromatography-mass spectrometry	767:802	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	3	74	theme	structural	581:590	arg1	characteristics					592:606	The structural characteristics	577:606	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages	577:682	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	3	75	theme	spectroscopy	831:842	arg1	detections					876:885	multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	738:885	multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	738:885	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	0	76	from	extraction	36:45	arg1	perilla					71:77	perilla	71:77	perilla	71:77	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	7	77	theme	perilla	1244:1250	arg1	meal					1257:1260	raw material perilla seed meal	1231:1260	raw material perilla seed meal	1231:1260	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	0	78	theme	response	92:99	arg1	methodology					109:119	response surface methodology	92:119	response surface methodology	92:119	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	0	79	dep	in	143:144	arg1	vitro					146:150	vitro	146:150	vitro	146:150	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	2	80	theme	52.00 min	473:481	arg1	temperature					429:439	ultrasonic temperature	418:439	ultrasonic temperature of 43.00 °C	418:451	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	80	theme	52.00 min	473:481	arg1	ratio					397:401	liquid-solid ratio	384:401	liquid-solid ratio of 26.00 mL/g	384:415	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	80	theme	52.00 min	473:481	arg1	conditions					530:539	the optimal conditions	518:539	the optimal conditions	518:539	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	80	theme	52.00 min	473:481	arg1	conditions					351:360	The optimal conditions	339:360	The optimal conditions for UAE of PSMP were	339:381	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	80	theme	52.00 min	473:481	arg1	time					465:468	ultrasonic time	454:468	ultrasonic time of 52.00 min	454:481	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	2	80	theme	52.00 min	473:481	arg1	power					499:503	ultrasonic power	488:503	ultrasonic power of 229.00 W	488:515	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	11	81	theme	response	1768:1775	arg1	methodology					1785:1795	response surface methodology	1768:1795	response surface methodology	1768:1795	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	3	82	theme	magnetic	857:864	arg1	resonance					866:874	nuclear magnetic resonance	849:874	nuclear magnetic resonance	849:874	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	7	83	theme	material	1235:1242	arg1	meal					1257:1260	raw material perilla seed meal	1231:1260	raw material perilla seed meal	1231:1260	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	0	84	from	perilla	71:77	arg1	polysaccharides					50:64	polysaccharides	50:64	polysaccharides from perilla	50:77	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	0	84	from	perilla	71:77	arg1	extraction					36:45	ultrasound-assisted extraction	16:45	ultrasound-assisted extraction of polysaccharides from perilla	16:77	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	6	85	theme	antioxidants	1143:1154	arg1	resource					1131:1138	potential natural resource	1113:1138	potential natural resource of antioxidants for medicine and functional foods	1113:1188	The results suggested that PSMP is potential natural resource of antioxidants for medicine and functional foods.					
33462808	5	86	theme	antioxidant	998:1008	arg1	activities					1010:1019	The antioxidant activities	994:1019	The antioxidant activities of PSMP	994:1027	The antioxidant activities of PSMP were investigated using various assays in vitro.					
33462808	10	87	theme	Ultrasound-assisted	1543:1561	arg1	method					1607:1612	a promising alternative method	1583:1612	a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving	1583:1722	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	10	87	theme	Ultrasound-assisted	1543:1561	arg1	UAE					1575:1577	UAE	1575:1577	UAE	1575:1577	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	10	87	theme	Ultrasound-assisted	1543:1561	arg1	extraction					1563:1572	Ultrasound-assisted extraction	1543:1572	Ultrasound-assisted extraction (UAE)	1543:1578	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	8	88	theme	wholesome	1391:1399	arg1	ingredients					1401:1411	wholesome ingredients	1391:1411	wholesome ingredients	1391:1411	With consumer demands for newly developed foods with natural, wholesome ingredients are increasing nowadays.					
33462808	2	89	theme	liquid-solid	384:395	arg1	ratio					397:401	liquid-solid ratio	384:401	liquid-solid ratio of 26.00 mL/g	384:415	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	11	90	theme	total	1871:1875	arg1	cost					1877:1880	total cost	1871:1880	total cost of production	1871:1894	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	11	91	from	work	1733:1736	arg1	suitable					1805:1812	suitable	1805:1812	suitable	1805:1812	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	11	91	from	work	1733:1736	arg1	process					1747:1753	the UAE process	1739:1753	the UAE process optimized by response surface methodology	1739:1795	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	11	92	theme	surface	1777:1783	arg1	methodology					1785:1795	response surface methodology	1768:1795	response surface methodology	1768:1795	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	10	93	theme	energy	1710:1715	arg1	saving					1717:1722	energy saving	1710:1722	energy saving	1710:1722	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	0	94	theme	extraction	36:45	arg1	Optimization					0:11	Optimization	0:11	Optimization of ultrasound-assisted extraction of polysaccharides from perilla	0:77	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	1	95	theme	perilla	297:303	arg1	PSMP					332:335	PSMP	332:335	PSMP	332:335	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	1	95	theme	perilla	297:303	arg1	polysaccharides					315:329	perilla seed meal polysaccharides	297:329	perilla seed meal polysaccharides (PSMP)	297:336	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	3	96	theme	weight	621:626	arg1	characteristics					592:606	The structural characteristics	577:606	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages	577:682	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	0	97	theme	polysaccharides	50:64	arg1	extraction					36:45	ultrasound-assisted extraction	16:45	ultrasound-assisted extraction of polysaccharides from perilla	16:77	Optimization of ultrasound-assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities.					
33462808	1	98	theme	meal	310:313	arg1	PSMP					332:335	PSMP	332:335	PSMP	332:335	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	1	98	theme	meal	310:313	arg1	polysaccharides					315:329	perilla seed meal polysaccharides	297:329	perilla seed meal polysaccharides (PSMP)	297:336	In this study, response surface methodology was employed to optimize the ultrasound-assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP).					
33462808	11	99	theme	UAE	1743:1745	arg1	process					1747:1753	the UAE process	1739:1753	the UAE process optimized by response surface methodology	1739:1795	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	11	99	theme	UAE	1743:1745	arg1	suitable					1805:1812	suitable	1805:1812	suitable	1805:1812	In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.					
33462808	7	100	theme	resources	1318:1326	arg1	utilization					1296:1306	the comprehensive utilization	1278:1306	the comprehensive utilization of edible resources	1278:1326	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	10	101	theme	alternative	1595:1605	arg1	extraction					1563:1572	Ultrasound-assisted extraction	1543:1572	Ultrasound-assisted extraction (UAE)	1543:1578	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	10	101	theme	alternative	1595:1605	arg1	method					1607:1612	a promising alternative method	1583:1612	a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving	1583:1722	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	2	102	theme	optimal	343:349	arg1	conditions					351:360	The optimal conditions	339:360	The optimal conditions for UAE of PSMP were	339:381	The optimal conditions for UAE of PSMP were: liquid-solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%.					
33462808	3	103	theme	compositional	629:641	arg1	monosaccharides					643:657	compositional monosaccharides	629:657	compositional monosaccharides	629:657	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
33462808	7	104	theme	comprehensive	1282:1294	arg1	utilization					1296:1306	the comprehensive utilization	1278:1306	the comprehensive utilization of edible resources	1278:1326	PRACTICAL APPLICATION: The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources.					
33462808	10	105	theme	water	1622:1626	arg1	HWRE					1647:1650	HWRE	1647:1650	HWRE	1647:1650	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	10	105	theme	water	1622:1626	arg1	extraction					1635:1644	hot water reflux extraction	1618:1644	hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving	1618:1722	Ultrasound-assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving.					
33462808	9	106	theme	effective	1458:1466	arg1	reference					1468:1476	effective reference	1458:1476	effective reference for in-depth research on other medicine-food dual-use resources	1458:1540	This study provides effective reference for in-depth research on other medicine-food dual-use resources.					
33462808	3	107	theme	spectrometry	791:802	arg1	detections					876:885	multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	738:885	multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections	738:885	The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography-mass spectrometry, Fourier-transfer infrared spectroscopy, and nuclear magnetic resonance detections.					
31915280	14	0	theme	host	2557:2560	arg1	molecules					2562:2570	the host molecules	2553:2570	the host molecules exploited by DENV and other flaviviruses to facilitate their life cycle	2553:2642	These results provide insights into the host molecules exploited by DENV and other flaviviruses to facilitate their life cycle.					
31915280	12	1	theme	host	2248:2251	arg1	factors					2253:2259	important host factors	2238:2259	important host factors for the replication of DENV as well as other related viruses such as Zika virus	2238:2339	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	1	theme	host	2248:2251	arg1	-3					2197:2198	-3	2197:2198	-3	2197:2198	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	1	theme	host	2248:2251	arg1	virus					2335:2339	Zika virus	2330:2339	Zika virus	2330:2339	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	1	theme	host	2248:2251	arg1	DPM1					2188:2191	DPM1	2188:2191	DPM1	2188:2191	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	10	2	theme	life	1993:1996	arg1	cycle					1998:2002	its life cycle	1989:2002	its life cycle	1989:2002	DENV encodes only few proteins and relies on the host cell machinery to accomplish its life cycle.					
31915280	1	3	theme	health	245:250	arg1	disease					222:228	dengue disease	215:228	dengue disease	215:228	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	1	3	theme	health	245:250	arg1	concern					252:258	a major human health concern	231:258	a major human health concern for which no specific therapies are available	231:304	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	3	4	theme	DENV	527:530	arg1	infection					532:540	DENV infection	527:540	DENV infection	527:540	In this study, we performed a genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify host genes important for DENV infection.					
31915280	11	5	theme	important	2044:2052	arg1	factors					2036:2042	the host factors	2027:2042	the host factors important for DENV infection	2027:2071	The identification of the host factors important for DENV infection is needed to propose new targets for antiviral intervention.					
31915280	9	6	theme	mosquito-borne	1829:1842	arg1	disease					1850:1856	the most important mosquito-borne viral disease	1810:1856	the most important mosquito-borne viral disease in humans	1810:1866	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	9	6	theme	mosquito-borne	1829:1842	arg1	disease					1746:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	4	7	theme	resident	617:624	arg1	complex					669:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	13	8	theme	dual	2381:2384	arg1	roles					2386:2390	dual roles	2381:2390	dual roles	2381:2390	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	3	9	theme	important	513:521	arg1	genes					507:511	host genes	502:511	host genes important for DENV infection	502:540	In this study, we performed a genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify host genes important for DENV infection.					
31915280	11	10	theme	host	2031:2034	arg1	factors					2036:2042	the host factors	2027:2042	the host factors important for DENV infection	2027:2071	The identification of the host factors important for DENV infection is needed to propose new targets for antiviral intervention.					
31915280	4	11	theme	mannose	645:651	arg1	complex					669:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	5	12	from	biosynthesis	964:975	arg1	lumen					1026:1030	the ER lumen	1019:1030	the ER lumen	1019:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	1	13	theme	mosquito-borne	173:186	arg1	virus					155:159	Dengue virus	148:159	Dengue virus (DENV)	148:166	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	1	13	theme	mosquito-borne	173:186	arg1	flavivirus					188:197	a mosquito-borne flavivirus	171:197	a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available	171:304	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	9	14	theme	DENV	1614:1617	arg1	infection					1619:1627	DENV infection	1614:1627	DENV infection	1614:1627	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	6	15	theme	DPM1	1062:1065	arg1	motif					1053:1057	the DXD motif	1045:1057	the DXD motif of DPM1	1045:1065	Mutation in the DXD motif of DPM1, which is essential for its catalytic activity, abolished DPMS-mediated DENV infection.					
31915280	12	16	theme	Zika	2330:2333	arg1	virus					2335:2339	Zika virus	2330:2339	Zika virus	2330:2339	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	4	17	theme	reticulum	602:610	arg1	complex					669:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	5	18	theme	mannosyl	867:874	arg1	donor					876:880	mannosyl donor	867:880	mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen	867:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	0	19	theme	Dengue	124:129	arg1	Infection					137:145	Dengue Virus Infection	124:145	Dengue Virus Infection	124:145	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	1	20	theme	responsible	199:209	arg1	virus					155:159	Dengue virus	148:159	Dengue virus (DENV)	148:166	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	1	20	theme	responsible	199:209	arg1	flavivirus					188:197	a mosquito-borne flavivirus	171:197	a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available	171:304	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	8	21	theme	deficient	1353:1361	arg1	cells					1347:1351	cells	1347:1351	cells deficient for DPMS activity	1347:1379	We also established that in cells deficient for DPMS activity, viral RNA amplification is hampered and truncated oligosaccharides are transferred to the viral prM and E glycoproteins, affecting their proper folding.					
31915280	14	22	theme	life	2633:2636	arg1	cycle					2638:2642	their life cycle	2627:2642	their life cycle	2627:2642	These results provide insights into the host molecules exploited by DENV and other flaviviruses to facilitate their life cycle.					
31915280	1	23	theme	dengue	215:220	arg1	disease					222:228	dengue disease	215:228	dengue disease	215:228	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	1	23	theme	dengue	215:220	arg1	concern					252:258	a major human health concern	231:258	a major human health concern for which no specific therapies are available	231:304	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	9	24	theme	current	1642:1648	arg1	approaches					1662:1671	current therapeutic approaches	1642:1671	current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern	1642:1903	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	8	25	theme	DPMS	1367:1370	arg1	activity					1372:1379	DPMS activity	1367:1379	DPMS activity	1367:1379	We also established that in cells deficient for DPMS activity, viral RNA amplification is hampered and truncated oligosaccharides are transferred to the viral prM and E glycoproteins, affecting their proper folding.					
31915280	6	26	theme	DENV	1139:1142	arg1	infection					1144:1152	DPMS-mediated DENV infection	1125:1152	DPMS-mediated DENV infection	1125:1152	Mutation in the DXD motif of DPM1, which is essential for its catalytic activity, abolished DPMS-mediated DENV infection.					
31915280	9	27	theme	major	1877:1881	arg1	concern					1897:1903	a major global health concern	1875:1903	a major global health concern	1875:1903	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	9	28	theme	health	1890:1895	arg1	concern					1897:1903	a major global health concern	1875:1903	a major global health concern	1875:1903	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	4	29	theme	host	681:684	arg1	-3					566:567	-3	566:567	-3	566:567	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	4	29	theme	host	681:684	arg1	DPM1					557:560	DPM1	557:560	DPM1	557:560	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	4	29	theme	host	681:684	arg1	factors					697:703	host dependency factors	681:703	host dependency factors	681:703	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	4	29	theme	host	681:684	arg1	virus					759:763	Zika virus	754:763	Zika virus (ZIKV)	754:770	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	9	30	theme	glycosylation	1679:1691	arg1	inhibitors					1693:1702	glycosylation inhibitors	1679:1702	glycosylation inhibitors	1679:1702	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	8	31	theme	RNA	1388:1390	arg1	amplification					1392:1404	viral RNA amplification	1382:1404	viral RNA amplification	1382:1404	We also established that in cells deficient for DPMS activity, viral RNA amplification is hampered and truncated oligosaccharides are transferred to the viral prM and E glycoproteins, affecting their proper folding.					
31915280	13	32	gly	glycoprotein	2485:2496	arg1	glycoprotein					2485:2496	viral structural glycoprotein folding/stability	2468:2514	viral structural glycoprotein folding/stability	2468:2514	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	10	33	theme	few	1924:1926	arg1	proteins					1928:1935	only few proteins	1919:1935	only few proteins	1919:1935	DENV encodes only few proteins and relies on the host cell machinery to accomplish its life cycle.					
31915280	10	33	theme	few	1924:1926	arg1	DENV					1906:1909	DENV	1906:1909	DENV	1906:1909	DENV encodes only few proteins and relies on the host cell machinery to accomplish its life cycle.					
31915280	5	34	from	O-mannosylation	988:1002	arg1	lumen					1026:1030	the ER lumen	1019:1030	the ER lumen	1019:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	0	35	theme	CRISPR-Cas9	14:24	arg1	Screen					26:31	A Genome-Wide CRISPR-Cas9 Screen	0:31	A Genome-Wide CRISPR-Cas9 Screen	0:31	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	5	36	theme	mannose	836:842	arg1	synthesis					804:812	the synthesis	800:812	the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen	800:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	5	37	gly	N-glycosylation	905:919	arg1	proteins					1007:1014	proteins	1007:1014	proteins in the ER lumen	1007:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	10	38	theme	host	1955:1958	arg1	machinery					1965:1973	the host cell machinery	1951:1973	the host cell machinery	1951:1973	DENV encodes only few proteins and relies on the host cell machinery to accomplish its life cycle.					
31915280	5	39	from	C-	982:983	arg1	lumen					1026:1030	the ER lumen	1019:1030	the ER lumen	1019:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	9	40	theme	DENV	1713:1716	arg1	disease					1850:1856	the most important mosquito-borne viral disease	1810:1856	the most important mosquito-borne viral disease in humans	1810:1866	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	9	40	theme	DENV	1713:1716	arg1	disease					1746:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	4	41	theme	other	718:722	arg1	flaviviruses					732:743	other related flaviviruses	718:743	other related flaviviruses	718:743	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	0	42	theme	Host	97:100	arg1	Factor					113:118	a Host Dependency Factor	95:118	a Host Dependency Factor for Dengue Virus Infection	95:145	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	0	42	theme	Host	97:100	arg1	Complex					84:90	the Dolichol-Phosphate Mannose Synthase Complex	44:90	the Dolichol-Phosphate Mannose Synthase Complex	44:90	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	5	43	theme	ER	1023:1024	arg1	lumen					1026:1030	the ER lumen	1019:1030	the ER lumen	1019:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	9	44	theme	Dengue	1739:1744	arg1	disease					1850:1856	the most important mosquito-borne viral disease	1810:1856	the most important mosquito-borne viral disease in humans	1810:1866	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	9	44	theme	Dengue	1739:1744	arg1	disease					1746:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	12	45	theme	CRISPR-Cas9	2154:2164	arg1	screen					2166:2171	a genome-wide CRISPR-Cas9 screen	2140:2171	a genome-wide CRISPR-Cas9 screen	2140:2171	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	5	46	from	proteins	1007:1014	arg1	lumen					1026:1030	the ER lumen	1019:1030	the ER lumen	1019:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	2	47	theme	cellular	359:366	arg1	machinery					368:376	the host cellular machinery	350:376	the host cellular machinery for productive infection	350:401	Like other viruses, DENV relies heavily on the host cellular machinery for productive infection.					
31915280	8	48	gly	glycoproteins	1488:1500	arg1	glycoproteins					1488:1500	the viral prM and E glycoproteins	1468:1500	the viral prM and E glycoproteins	1468:1500	We also established that in cells deficient for DPMS activity, viral RNA amplification is hampered and truncated oligosaccharides are transferred to the viral prM and E glycoproteins, affecting their proper folding.					
31915280	7	49	theme	lipid-linked	1240:1251	arg1	LLO					1270:1272	LLO	1270:1272	LLO	1270:1272	Similarly, genetic ablation of ALG3, a mannosyltransferase that transfers mannose to lipid-linked oligosaccharide (LLO), rendered cells poorly susceptible to DENV.					
31915280	7	49	theme	lipid-linked	1240:1251	arg1	oligosaccharide					1253:1267	lipid-linked oligosaccharide	1240:1267	lipid-linked oligosaccharide (LLO)	1240:1273	Similarly, genetic ablation of ALG3, a mannosyltransferase that transfers mannose to lipid-linked oligosaccharide (LLO), rendered cells poorly susceptible to DENV.					
31915280	11	50	theme	DENV	2058:2061	arg1	infection					2063:2071	DENV infection	2058:2071	DENV infection	2058:2071	The identification of the host factors important for DENV infection is needed to propose new targets for antiviral intervention.					
31915280	12	51	theme	related	2306:2312	arg1	viruses					2314:2320	other related viruses	2300:2320	important host factors for the replication of DENV as well as other related viruses such as Zika virus	2238:2339	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	51	theme	related	2306:2312	arg1	-3					2197:2198	-3	2197:2198	-3	2197:2198	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	51	theme	related	2306:2312	arg1	virus					2335:2339	Zika virus	2330:2339	Zika virus	2330:2339	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	51	theme	related	2306:2312	arg1	DPM1					2188:2191	DPM1	2188:2191	DPM1	2188:2191	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	3	52	theme	genome-wide	434:444	arg1	screen					458:463	a genome-wide CRISPR-Cas9 screen	432:463	a genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify host genes important for DENV infection	432:540	In this study, we performed a genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify host genes important for DENV infection.					
31915280	5	53	from	lumen	1026:1030	arg1	O-mannosylation					988:1002	O-mannosylation	988:1002	O-mannosylation	988:1002	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	5	53	from	lumen	1026:1030	arg1	biosynthesis					964:975	glycosylphosphatidylinositol (GPI) anchor biosynthesis	922:975	glycosylphosphatidylinositol (GPI) anchor biosynthesis	922:975	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	5	53	from	lumen	1026:1030	arg1	C-					982:983	C-	982:983	C-	982:983	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	5	53	from	lumen	1026:1030	arg1	N-glycosylation					905:919	N-glycosylation	905:919	N-glycosylation	905:919	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	1	54	theme	specific	273:280	arg1	therapies					282:290	no specific therapies	270:290	no specific therapies	270:290	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	1	55	theme	Dengue	148:153	arg1	virus					155:159	Dengue virus	148:159	Dengue virus (DENV)	148:166	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	1	55	theme	Dengue	148:153	arg1	flavivirus					188:197	a mosquito-borne flavivirus	171:197	a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available	171:304	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	1	55	theme	Dengue	148:153	arg1	DENV					162:165	DENV	162:165	DENV	162:165	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	11	56	theme	new	2094:2096	arg1	targets					2098:2104	new targets	2094:2104	new targets for antiviral intervention	2094:2131	The identification of the host factors important for DENV infection is needed to propose new targets for antiviral intervention.					
31915280	8	57	theme	prM	1478:1480	arg1	glycoproteins					1488:1500	the viral prM and E glycoproteins	1468:1500	the viral prM and E glycoproteins	1468:1500	We also established that in cells deficient for DPMS activity, viral RNA amplification is hampered and truncated oligosaccharides are transferred to the viral prM and E glycoproteins, affecting their proper folding.					
31915280	13	58	theme	viral	2432:2436	arg1	replication					2442:2452	viral RNA replication	2432:2452	viral RNA replication	2432:2452	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	5	59	theme	anchor	957:962	arg1	biosynthesis					964:975	glycosylphosphatidylinositol (GPI) anchor biosynthesis	922:975	glycosylphosphatidylinositol (GPI) anchor biosynthesis	922:975	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	13	60	dep	plays	2375:2379	arg1	promoting					2458:2466	promoting	2458:2466	promoting viral structural glycoprotein folding/stability	2458:2514	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	13	60	dep	plays	2375:2379	arg1	regulating					2421:2430	regulating	2421:2430	regulating viral RNA replication	2421:2452	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	8	61	theme	E	1486:1486	arg1	glycoproteins					1488:1500	the viral prM and E glycoproteins	1468:1500	the viral prM and E glycoproteins	1468:1500	We also established that in cells deficient for DPMS activity, viral RNA amplification is hampered and truncated oligosaccharides are transferred to the viral prM and E glycoproteins, affecting their proper folding.					
31915280	0	62	theme	Synthase	75:82	arg1	Factor					113:118	a Host Dependency Factor	95:118	a Host Dependency Factor for Dengue Virus Infection	95:145	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	0	62	theme	Synthase	75:82	arg1	Complex					84:90	the Dolichol-Phosphate Mannose Synthase Complex	44:90	the Dolichol-Phosphate Mannose Synthase Complex	44:90	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	3	63	theme	haploid	471:477	arg1	cells					484:488	haploid HAP1 cells	471:488	haploid HAP1 cells	471:488	In this study, we performed a genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify host genes important for DENV infection.					
31915280	0	64	theme	Dolichol-Phosphate	48:65	arg1	Factor					113:118	a Host Dependency Factor	95:118	a Host Dependency Factor for Dengue Virus Infection	95:145	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	0	64	theme	Dolichol-Phosphate	48:65	arg1	Complex					84:90	the Dolichol-Phosphate Mannose Synthase Complex	44:90	the Dolichol-Phosphate Mannose Synthase Complex	44:90	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	9	65	theme	host-dependent	1585:1598	arg1	mechanisms					1600:1609	the host-dependent mechanisms	1581:1609	the host-dependent mechanisms of DENV infection	1581:1627	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	6	66	theme	DXD	1049:1051	arg1	motif					1053:1057	the DXD motif	1045:1057	the DXD motif of DPM1	1045:1065	Mutation in the DXD motif of DPM1, which is essential for its catalytic activity, abolished DPMS-mediated DENV infection.					
31915280	5	67	theme	glycosylphosphatidylinositol	922:949	arg1	biosynthesis					964:975	glycosylphosphatidylinositol (GPI) anchor biosynthesis	922:975	glycosylphosphatidylinositol (GPI) anchor biosynthesis	922:975	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	7	68	theme	ALG3	1186:1189	arg1	ablation					1174:1181	genetic ablation	1166:1181	genetic ablation of ALG3, a mannosyltransferase that transfers mannose to lipid-linked oligosaccharide (LLO),	1166:1274	Similarly, genetic ablation of ALG3, a mannosyltransferase that transfers mannose to lipid-linked oligosaccharide (LLO), rendered cells poorly susceptible to DENV.					
31915280	4	69	theme	Zika	754:757	arg1	ZIKV					766:769	ZIKV	766:769	ZIKV	766:769	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	4	69	theme	Zika	754:757	arg1	virus					759:763	Zika virus	754:763	Zika virus (ZIKV)	754:770	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	13	70	theme	RNA	2438:2440	arg1	replication					2442:2452	viral RNA replication	2432:2452	viral RNA replication	2432:2452	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	5	71	theme	GPI	952:954	arg1	biosynthesis					964:975	glycosylphosphatidylinositol (GPI) anchor biosynthesis	922:975	glycosylphosphatidylinositol (GPI) anchor biosynthesis	922:975	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	13	72	theme	viral	2399:2403	arg1	infection					2405:2413	viral infection	2399:2413	viral infection	2399:2413	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	5	73	from	N-glycosylation	905:919	arg1	lumen					1026:1030	the ER lumen	1019:1030	the ER lumen	1019:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	1	74	theme	human	239:243	arg1	disease					222:228	dengue disease	215:228	dengue disease	215:228	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	1	74	theme	human	239:243	arg1	concern					252:258	a major human health concern	231:258	a major human health concern for which no specific therapies are available	231:304	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	9	75	theme	new	1563:1565	arg1	insights					1567:1574	new insights	1563:1574	new insights into the host-dependent mechanisms of DENV infection	1563:1627	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	5	76	gly	O-mannosylation	988:1002	arg1	proteins					1007:1014	proteins	1007:1014	proteins in the ER lumen	1007:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	12	77	theme	important	2238:2246	arg1	factors					2253:2259	important host factors	2238:2259	important host factors for the replication of DENV as well as other related viruses such as Zika virus	2238:2339	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	77	theme	important	2238:2246	arg1	-3					2197:2198	-3	2197:2198	-3	2197:2198	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	77	theme	important	2238:2246	arg1	virus					2335:2339	Zika virus	2330:2339	Zika virus	2330:2339	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	77	theme	important	2238:2246	arg1	DPM1					2188:2191	DPM1	2188:2191	DPM1	2188:2191	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	9	78	theme	important	1819:1827	arg1	disease					1850:1856	the most important mosquito-borne viral disease	1810:1856	the most important mosquito-borne viral disease in humans	1810:1866	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	9	78	theme	important	1819:1827	arg1	disease					1746:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	7	79	link	lipid-linked	1240:1251	arg1	LLO					1270:1272	LLO	1270:1272	LLO	1270:1272	Similarly, genetic ablation of ALG3, a mannosyltransferase that transfers mannose to lipid-linked oligosaccharide (LLO), rendered cells poorly susceptible to DENV.					
31915280	7	79	link	lipid-linked	1240:1251	arg1	oligosaccharide					1253:1267	lipid-linked oligosaccharide	1240:1267	lipid-linked oligosaccharide (LLO)	1240:1273	Similarly, genetic ablation of ALG3, a mannosyltransferase that transfers mannose to lipid-linked oligosaccharide (LLO), rendered cells poorly susceptible to DENV.					
31915280	9	80	theme	viral	1844:1848	arg1	disease					1850:1856	the most important mosquito-borne viral disease	1810:1856	the most important mosquito-borne viral disease in humans	1810:1866	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	9	80	theme	viral	1844:1848	arg1	disease					1746:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	7	81	dep	susceptible	1298:1308	arg1	cells					1285:1289	cells	1285:1289	cells	1285:1289	Similarly, genetic ablation of ALG3, a mannosyltransferase that transfers mannose to lipid-linked oligosaccharide (LLO), rendered cells poorly susceptible to DENV.					
31915280	11	82	theme	factors	2036:2042	arg1	identification					2009:2022	The identification	2005:2022	The identification of the host factors important for DENV infection	2005:2071	The identification of the host factors important for DENV infection is needed to propose new targets for antiviral intervention.					
31915280	4	83	theme	dolichol-phosphate	626:643	arg1	complex					669:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	0	84	theme	Virus	131:135	arg1	Infection					137:145	Dengue Virus Infection	124:145	Dengue Virus Infection	124:145	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	9	85	theme	infection	1619:1627	arg1	mechanisms					1600:1609	the host-dependent mechanisms	1581:1609	the host-dependent mechanisms of DENV infection	1581:1627	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	12	86	theme	complex	2226:2232	arg1	subunits					2205:2212	two subunits	2201:2212	two subunits of the DPMS complex	2201:2232	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	86	theme	complex	2226:2232	arg1	DPM1					2188:2191	DPM1	2188:2191	DPM1	2188:2191	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	4	87	theme	endoplasmic	590:600	arg1	ER					613:614	ER	613:614	ER	613:614	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	4	87	theme	endoplasmic	590:600	arg1	reticulum					602:610	the endoplasmic reticulum	586:610	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	12	88	theme	DPMS	2221:2224	arg1	complex					2226:2232	the DPMS complex	2217:2232	the DPMS complex	2217:2232	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	13	89	theme	DPMS	2362:2365	arg1	complex					2367:2373	DPMS complex	2362:2373	DPMS complex	2362:2373	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	5	90	theme	proteins	1007:1014	arg1	O-mannosylation					988:1002	O-mannosylation	988:1002	O-mannosylation	988:1002	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	5	90	theme	proteins	1007:1014	arg1	biosynthesis					964:975	glycosylphosphatidylinositol (GPI) anchor biosynthesis	922:975	glycosylphosphatidylinositol (GPI) anchor biosynthesis	922:975	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	5	90	theme	proteins	1007:1014	arg1	C-					982:983	C-	982:983	C-	982:983	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	5	90	theme	proteins	1007:1014	arg1	N-glycosylation					905:919	N-glycosylation	905:919	N-glycosylation	905:919	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	5	91	theme	DPMS	777:780	arg1	complex					782:788	The DPMS complex	773:788	The DPMS complex	773:788	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	13	92	theme	glycoprotein	2485:2496	arg1	folding/stability					2498:2514	viral structural glycoprotein folding/stability	2468:2514	viral structural glycoprotein folding/stability	2468:2514	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	9	93	theme	therapeutic	1650:1660	arg1	approaches					1662:1671	current therapeutic approaches	1642:1671	current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern	1642:1903	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	8	94	theme	viral	1382:1386	arg1	amplification					1392:1404	viral RNA amplification	1382:1404	viral RNA amplification	1382:1404	We also established that in cells deficient for DPMS activity, viral RNA amplification is hampered and truncated oligosaccharides are transferred to the viral prM and E glycoproteins, affecting their proper folding.					
31915280	6	95	theme	DPMS-mediated	1125:1137	arg1	infection					1144:1152	DPMS-mediated DENV infection	1125:1152	DPMS-mediated DENV infection	1125:1152	Mutation in the DXD motif of DPM1, which is essential for its catalytic activity, abolished DPMS-mediated DENV infection.					
31915280	9	96	theme	global	1883:1888	arg1	concern					1897:1903	a major global health concern	1875:1903	a major global health concern	1875:1903	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	4	97	theme	complex	669:675	arg1	subunits					574:581	two subunits	570:581	two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	570:675	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	4	97	theme	complex	669:675	arg1	DPM1					557:560	DPM1	557:560	DPM1	557:560	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	9	98	from	disease	1850:1856	arg1	humans					1861:1866	humans	1861:1866	humans	1861:1866	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	5	99	theme	dolichol-phosphate	817:834	arg1	DPM					845:847	DPM	845:847	DPM	845:847	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	5	99	theme	dolichol-phosphate	817:834	arg1	mannose					836:842	dolichol-phosphate mannose	817:842	dolichol-phosphate mannose (DPM)	817:848	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	6	100	theme	catalytic	1095:1103	arg1	activity					1105:1112	its catalytic activity	1091:1112	its catalytic activity	1091:1112	Mutation in the DXD motif of DPM1, which is essential for its catalytic activity, abolished DPMS-mediated DENV infection.					
31915280	4	101	theme	synthase	653:660	arg1	complex					669:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	11	102	theme	antiviral	2110:2118	arg1	intervention					2120:2131	antiviral intervention	2110:2131	antiviral intervention	2110:2131	The identification of the host factors important for DENV infection is needed to propose new targets for antiviral intervention.					
31915280	8	103	theme	truncated	1422:1430	arg1	oligosaccharides					1432:1447	truncated oligosaccharides	1422:1447	truncated oligosaccharides	1422:1447	We also established that in cells deficient for DPMS activity, viral RNA amplification is hampered and truncated oligosaccharides are transferred to the viral prM and E glycoproteins, affecting their proper folding.					
31915280	4	104	theme	DPMS	663:666	arg1	complex					669:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex	586:675	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	0	105	theme	Dependency	102:111	arg1	Factor					113:118	a Host Dependency Factor	95:118	a Host Dependency Factor for Dengue Virus Infection	95:145	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	0	105	theme	Dependency	102:111	arg1	Complex					84:90	the Dolichol-Phosphate Mannose Synthase Complex	44:90	the Dolichol-Phosphate Mannose Synthase Complex	44:90	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	9	106	theme	infection.IMPORTANCE	1718:1737	arg1	disease					1850:1856	the most important mosquito-borne viral disease	1810:1856	the most important mosquito-borne viral disease in humans	1810:1866	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	9	106	theme	infection.IMPORTANCE	1718:1737	arg1	disease					1746:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	DENV infection.IMPORTANCE Dengue disease	1713:1752	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	4	107	theme	related	724:730	arg1	flaviviruses					732:743	other related flaviviruses	718:743	other related flaviviruses	718:743	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	0	108	theme	Genome-Wide	2:12	arg1	Screen					26:31	A Genome-Wide CRISPR-Cas9 Screen	0:31	A Genome-Wide CRISPR-Cas9 Screen	0:31	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	8	109	theme	viral	1472:1476	arg1	prM					1478:1480	viral prM	1472:1480	viral prM	1472:1480	We also established that in cells deficient for DPMS activity, viral RNA amplification is hampered and truncated oligosaccharides are transferred to the viral prM and E glycoproteins, affecting their proper folding.					
31915280	4	110	theme	dependency	686:695	arg1	-3					566:567	-3	566:567	-3	566:567	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	4	110	theme	dependency	686:695	arg1	DPM1					557:560	DPM1	557:560	DPM1	557:560	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	4	110	theme	dependency	686:695	arg1	factors					697:703	host dependency factors	681:703	host dependency factors	681:703	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	4	110	theme	dependency	686:695	arg1	virus					759:763	Zika virus	754:763	Zika virus (ZIKV)	754:770	We identified DPM1 and -3, two subunits of the endoplasmic reticulum (ER) resident dolichol-phosphate mannose synthase (DPMS) complex, as host dependency factors for DENV and other related flaviviruses, such as Zika virus (ZIKV).					
31915280	2	111	theme	host	354:357	arg1	machinery					368:376	the host cellular machinery	350:376	the host cellular machinery for productive infection	350:401	Like other viruses, DENV relies heavily on the host cellular machinery for productive infection.					
31915280	13	112	theme	viral	2468:2472	arg1	folding/stability					2498:2514	viral structural glycoprotein folding/stability	2468:2514	viral structural glycoprotein folding/stability	2468:2514	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	13	113	theme	structural	2474:2483	arg1	folding/stability					2498:2514	viral structural glycoprotein folding/stability	2468:2514	viral structural glycoprotein folding/stability	2468:2514	We established that DPMS complex plays dual roles during viral infection, both regulating viral RNA replication and promoting viral structural glycoprotein folding/stability.					
31915280	3	114	theme	CRISPR-Cas9	446:456	arg1	screen					458:463	a genome-wide CRISPR-Cas9 screen	432:463	a genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify host genes important for DENV infection	432:540	In this study, we performed a genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify host genes important for DENV infection.					
31915280	9	115	theme	dengue	1774:1779	arg1	DENV					1788:1791	DENV	1788:1791	DENV	1788:1791	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	9	115	theme	dengue	1774:1779	arg1	virus					1781:1785	dengue virus	1774:1785	dengue virus (DENV)	1774:1792	Overall, our study provides new insights into the host-dependent mechanisms of DENV infection and supports current therapeutic approaches using glycosylation inhibitors to treat DENV infection.IMPORTANCE Dengue disease, which is caused by dengue virus (DENV), has emerged as the most important mosquito-borne viral disease in humans and is a major global health concern.					
31915280	2	116	theme	productive	382:391	arg1	infection					393:401	productive infection	382:401	productive infection	382:401	Like other viruses, DENV relies heavily on the host cellular machinery for productive infection.					
31915280	0	117	theme	Mannose	67:73	arg1	Factor					113:118	a Host Dependency Factor	95:118	a Host Dependency Factor for Dengue Virus Infection	95:145	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	0	117	theme	Mannose	67:73	arg1	Complex					84:90	the Dolichol-Phosphate Mannose Synthase Complex	44:90	the Dolichol-Phosphate Mannose Synthase Complex	44:90	A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.					
31915280	14	118	theme	other	2594:2598	arg1	flaviviruses					2600:2611	other flaviviruses	2594:2611	other flaviviruses	2594:2611	These results provide insights into the host molecules exploited by DENV and other flaviviruses to facilitate their life cycle.					
31915280	12	119	theme	other	2300:2304	arg1	viruses					2314:2320	other related viruses	2300:2320	important host factors for the replication of DENV as well as other related viruses such as Zika virus	2238:2339	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	119	theme	other	2300:2304	arg1	-3					2197:2198	-3	2197:2198	-3	2197:2198	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	119	theme	other	2300:2304	arg1	virus					2335:2339	Zika virus	2330:2339	Zika virus	2330:2339	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	12	119	theme	other	2300:2304	arg1	DPM1					2188:2191	DPM1	2188:2191	DPM1	2188:2191	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	3	120	theme	HAP1	479:482	arg1	cells					484:488	haploid HAP1 cells	471:488	haploid HAP1 cells	471:488	In this study, we performed a genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify host genes important for DENV infection.					
31915280	5	121	from	donor	876:880	arg1	pathways					885:892	pathways	885:892	pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen	885:1030	The DPMS complex catalyzes the synthesis of dolichol-phosphate mannose (DPM), which serves as mannosyl donor in pathways leading to N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and C- or O-mannosylation of proteins in the ER lumen.					
31915280	8	122	theme	proper	1519:1524	arg1	folding					1526:1532	their proper folding	1513:1532	their proper folding	1513:1532	We also established that in cells deficient for DPMS activity, viral RNA amplification is hampered and truncated oligosaccharides are transferred to the viral prM and E glycoproteins, affecting their proper folding.					
31915280	12	123	theme	genome-wide	2142:2152	arg1	screen					2166:2171	a genome-wide CRISPR-Cas9 screen	2140:2171	a genome-wide CRISPR-Cas9 screen	2140:2171	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	2	124	theme	other	312:316	arg1	viruses					318:324	other viruses	312:324	other viruses	312:324	Like other viruses, DENV relies heavily on the host cellular machinery for productive infection.					
31915280	7	125	theme	genetic	1166:1172	arg1	ablation					1174:1181	genetic ablation	1166:1181	genetic ablation of ALG3, a mannosyltransferase that transfers mannose to lipid-linked oligosaccharide (LLO),	1166:1274	Similarly, genetic ablation of ALG3, a mannosyltransferase that transfers mannose to lipid-linked oligosaccharide (LLO), rendered cells poorly susceptible to DENV.					
31915280	10	126	theme	cell	1960:1963	arg1	machinery					1965:1973	the host cell machinery	1951:1973	the host cell machinery	1951:1973	DENV encodes only few proteins and relies on the host cell machinery to accomplish its life cycle.					
31915280	12	127	theme	DENV	2284:2287	arg1	replication					2269:2279	the replication	2265:2279	the replication of DENV	2265:2287	Using a genome-wide CRISPR-Cas9 screen, we identified DPM1 and -3, two subunits of the DPMS complex, as important host factors for the replication of DENV as well as other related viruses such as Zika virus.					
31915280	6	128	from	Mutation	1033:1040	arg1	motif					1053:1057	the DXD motif	1045:1057	the DXD motif of DPM1	1045:1065	Mutation in the DXD motif of DPM1, which is essential for its catalytic activity, abolished DPMS-mediated DENV infection.					
31915280	3	129	theme	host	502:505	arg1	genes					507:511	host genes	502:511	host genes important for DENV infection	502:540	In this study, we performed a genome-wide CRISPR-Cas9 screen using haploid HAP1 cells to identify host genes important for DENV infection.					
31915280	1	130	theme	major	233:237	arg1	disease					222:228	dengue disease	215:228	dengue disease	215:228	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
31915280	1	130	theme	major	233:237	arg1	concern					252:258	a major human health concern	231:258	a major human health concern for which no specific therapies are available	231:304	Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available.					
33303137	0	0	theme	cancer	90:95	arg1	subtypes					97:104	different breast cancer subtypes	73:104	different breast cancer subtypes	73:104	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	4	1	theme	glycan	708:713	arg1	composition					715:725	the glycan composition	704:725	the glycan composition	704:725	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	1	2	theme	heterogeneous	157:169	arg1	cancer					138:143	Breast cancer	131:143	Breast cancer	131:143	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	1	2	theme	heterogeneous	157:169	arg1	disease					171:177	a highly heterogeneous disease	148:177	a highly heterogeneous disease	148:177	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	7	3	theme	breast	1425:1430	arg1	subtypes					1439:1446	breast cancer subtypes	1425:1446	breast cancer subtypes	1425:1446	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	0	4	theme	breast	83:88	arg1	subtypes					97:104	different breast cancer subtypes	73:104	different breast cancer subtypes	73:104	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	0	5	from	analysis	12:19	arg1	receptor					58:65	mannose receptor	50:65	mannose receptor	50:65	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	2	6	from	sites	364:368	arg1	subtypes					418:425	different breast cancer subtypes	394:425	different breast cancer subtypes	394:425	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	2	7	theme	receptors	381:389	arg1	sites					364:368	several glycosylation sites	342:368	several glycosylation sites of mannose receptors in different breast cancer subtypes	342:425	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	2	7	theme	receptors	381:389	arg1	receptors					381:389	mannose receptors	373:389	mannose receptors	373:389	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	1	8	theme	biological	274:283	arg1	behaviors					285:293	biological behaviors	274:293	biological behaviors	274:293	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	6	9	theme	glycosylation	1065:1077	arg1	sites					1079:1083	7 glycosylation sites	1063:1083	7 glycosylation sites	1063:1083	In total, 7 glycosylation sites and 12 glycan types corresponding to 26 intact glycopeptides were characterized from the four subtypes of breast cancer.					
33303137	3	10	from	composition	532:542	arg1	site					552:555	each site	547:555	each site	547:555	However, the glycan composition on each site is still unknown because the glycan was removed by PNGase F in previous work.					
33303137	5	11	theme	breast	949:954	arg1	patients					963:970	breast cancer patients	949:970	breast cancer patients	949:970	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	5	12	theme	receptors	925:933	arg1	glycopeptides					896:908	the intact glycopeptides	885:908	the intact glycopeptides of the mannose receptors in serum from breast cancer patients	885:970	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	0	13	from	receptor	58:65	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes	0:104	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	0	13	from	receptor	58:65	arg1	glycopeptides					31:43	intact glycopeptides	24:43	intact glycopeptides from mannose receptor	24:65	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	5	14	theme	cancer	956:961	arg1	patients					963:970	breast cancer patients	949:970	breast cancer patients	949:970	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	2	15	theme	preliminary	303:313	arg1	experiments					315:325	the preliminary experiments	299:325	the preliminary experiments	299:325	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	5	16	theme	mannose	917:923	arg1	receptors					925:933	the mannose receptors	913:933	the mannose receptors	913:933	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	7	17	theme	cancer	1432:1437	arg1	subtypes					1439:1446	breast cancer subtypes	1425:1446	breast cancer subtypes	1425:1446	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	4	18	theme	breast	844:849	arg1	cancer					851:856	breast cancer	844:856	breast cancer	844:856	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	7	19	theme	breast	1280:1285	arg1	cancer					1287:1292	breast cancer	1280:1292	breast cancer	1280:1292	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	7	20	theme	potential	1382:1390	arg1	marker					1392:1397	a potential marker	1380:1397	a potential marker for the identification of breast cancer subtypes	1380:1446	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	7	20	theme	potential	1382:1390	arg1	receptor					1356:1363	mannose receptor	1348:1363	mannose receptor	1348:1363	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	0	21	theme	mass	112:115	arg1	spectrometry					117:128	mass spectrometry	112:128	mass spectrometry	112:128	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	6	22	theme	glycan	1092:1097	arg1	types					1099:1103	12 glycan types	1089:1103	12 glycan types	1089:1103	In total, 7 glycosylation sites and 12 glycan types corresponding to 26 intact glycopeptides were characterized from the four subtypes of breast cancer.					
33303137	6	23	gly	glycopeptides	1132:1144	arg2	glycopeptides					1132:1144	26 intact glycopeptides	1122:1144	26 intact glycopeptides	1122:1144	In total, 7 glycosylation sites and 12 glycan types corresponding to 26 intact glycopeptides were characterized from the four subtypes of breast cancer.					
33303137	2	24	theme	potential	476:484	arg1	marker					486:491	a potential marker	474:491	a potential marker for breast cancer	474:509	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	2	24	theme	potential	476:484	arg1	receptors					455:463	the mannose receptors	443:463	the mannose receptors	443:463	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	2	25	theme	several	342:348	arg1	sites					364:368	several glycosylation sites	342:368	several glycosylation sites of mannose receptors in different breast cancer subtypes	342:425	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	2	25	theme	several	342:348	arg1	receptors					381:389	mannose receptors	373:389	mannose receptors	373:389	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	7	26	theme	cancer	1287:1292	arg1	subtypes					1268:1275	the subtypes	1264:1275	the subtypes	1264:1275	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	0	27	theme	Comparative	0:10	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes	0:104	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	4	28	gly	glycopeptides	654:666	arg2	glycopeptides					654:666	intact glycopeptides	647:666	intact glycopeptides	647:666	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	5	29	from	glycopeptides	896:908	arg1	serum					938:942	serum	938:942	serum from breast cancer patients	938:970	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	5	29	from	glycopeptides	896:908	arg1	patients					963:970	breast cancer patients	949:970	breast cancer patients	949:970	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	4	30	theme	site	749:752	arg1	information					684:694	the information	680:694	the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer	680:856	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	2	31	theme	cancer	411:416	arg1	subtypes					418:425	different breast cancer subtypes	394:425	different breast cancer subtypes	394:425	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	0	32	theme	intact	24:29	arg1	glycopeptides					31:43	intact glycopeptides	24:43	intact glycopeptides from mannose receptor	24:65	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	4	33	gly	glycosylation	735:747	arg2	site					749:752	the glycosylation site	731:752	the glycosylation site	731:752	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	7	34	theme	mannose	1348:1354	arg1	marker					1392:1397	a potential marker	1380:1397	a potential marker for the identification of breast cancer subtypes	1380:1446	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	7	34	theme	mannose	1348:1354	arg1	receptor					1356:1363	mannose receptor	1348:1363	mannose receptor	1348:1363	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	7	35	used	used	1242:1245	arg2	glycopeptides					1221:1233	11 glycopeptides	1218:1233	11 glycopeptides	1218:1233	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	7	36	used	used	1372:1375	arg2	marker					1392:1397	a potential marker	1380:1397	a potential marker for the identification of breast cancer subtypes	1380:1446	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	7	36	used	used	1372:1375	arg2	receptor					1356:1363	mannose receptor	1348:1363	mannose receptor	1348:1363	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	6	37	theme	intact	1125:1130	arg1	glycopeptides					1132:1144	26 intact glycopeptides	1122:1144	26 intact glycopeptides	1122:1144	In total, 7 glycosylation sites and 12 glycan types corresponding to 26 intact glycopeptides were characterized from the four subtypes of breast cancer.					
33303137	1	38	with	entities	227:234	arg1	features					261:268	specific pathologic features	241:268	specific pathologic features	241:268	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	1	38	with	entities	227:234	arg1	behaviors					285:293	biological behaviors	274:293	biological behaviors	274:293	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	5	39	theme	intact	889:894	arg1	glycopeptides					896:908	the intact glycopeptides	885:908	the intact glycopeptides of the mannose receptors in serum from breast cancer patients	885:970	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	6	40	gly	glycosylation	1065:1077	arg2	7					1063:1063	7	1063:1063	7	1063:1063	In total, 7 glycosylation sites and 12 glycan types corresponding to 26 intact glycopeptides were characterized from the four subtypes of breast cancer.					
33303137	6	40	gly	glycosylation	1065:1077	arg2	sites					1079:1083	7 glycosylation sites	1063:1083	7 glycosylation sites	1063:1083	In total, 7 glycosylation sites and 12 glycan types corresponding to 26 intact glycopeptides were characterized from the four subtypes of breast cancer.					
33303137	2	41	theme	mannose	447:453	arg1	marker					486:491	a potential marker	474:491	a potential marker for breast cancer	474:509	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	2	41	theme	mannose	447:453	arg1	receptors					455:463	the mannose receptors	443:463	the mannose receptors	443:463	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	3	42	theme	glycan	525:530	arg1	composition					532:542	the glycan composition	521:542	the glycan composition on each site	521:555	However, the glycan composition on each site is still unknown because the glycan was removed by PNGase F in previous work.					
33303137	3	42	theme	glycan	525:530	arg1	unknown					566:572	unknown	566:572	unknown	566:572	However, the glycan composition on each site is still unknown because the glycan was removed by PNGase F in previous work.					
33303137	4	43	theme	composition	715:725	arg1	information					684:694	the information	680:694	the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer	680:856	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	4	44	theme	glycopeptides	654:666	arg1	Analysis					635:642	Analysis	635:642	Analysis of intact glycopeptides	635:666	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	3	45	theme	PNGase	608:613	arg1	F					615:615	PNGase F	608:615	PNGase F	608:615	However, the glycan composition on each site is still unknown because the glycan was removed by PNGase F in previous work.					
33303137	1	46	theme	distinct	218:225	arg1	entities					227:234	biologically distinct entities	205:234	biologically distinct entities with specific pathologic features and biological behaviors	205:293	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	0	47	theme	glycopeptides	31:43	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes	0:104	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	7	48	theme	subtypes	1439:1446	arg1	identification					1407:1420	the identification	1403:1420	the identification of breast cancer subtypes	1403:1446	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	2	49	theme	breast	497:502	arg1	cancer					504:509	breast cancer	497:509	breast cancer	497:509	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	5	50	theme	absolute	1009:1016	arg1	iTRAQ					1032:1036	iTRAQ	1032:1036	iTRAQ	1032:1036	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	5	50	theme	absolute	1009:1016	arg1	quantitation					1018:1029	relative and absolute quantitation	996:1029	relative and absolute quantitation (iTRAQ)	996:1037	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	1	51	theme	entities	227:234	arg1	number					195:200	a number	193:200	a number of biologically distinct entities with specific pathologic features and biological behaviors	193:293	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	5	52	gly	glycopeptides	896:908	arg1	receptors					925:933	the mannose receptors	913:933	the mannose receptors	913:933	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	5	52	gly	glycopeptides	896:908	arg2	glycopeptides					896:908	the intact glycopeptides	885:908	the intact glycopeptides of the mannose receptors in serum from breast cancer patients	885:970	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	4	53	theme	cancer	851:856	arg1	subtypes					832:839	the four subtypes	823:839	the four subtypes of breast cancer	823:856	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	2	54	theme	mannose	373:379	arg1	receptors					381:389	mannose receptors	373:389	mannose receptors	373:389	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	0	55	theme	mannose	50:56	arg1	receptor					58:65	mannose receptor	50:65	mannose receptor	50:65	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	6	56	theme	cancer	1198:1203	arg1	subtypes					1179:1186	the four subtypes	1170:1186	the four subtypes of breast cancer	1170:1203	In total, 7 glycosylation sites and 12 glycan types corresponding to 26 intact glycopeptides were characterized from the four subtypes of breast cancer.					
33303137	1	57	theme	Breast	131:136	arg1	cancer					138:143	Breast cancer	131:143	Breast cancer	131:143	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	1	57	theme	Breast	131:136	arg1	disease					171:177	a highly heterogeneous disease	148:177	a highly heterogeneous disease	148:177	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	5	58	theme	relative	996:1003	arg1	iTRAQ					1032:1036	iTRAQ	1032:1036	iTRAQ	1032:1036	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	5	58	theme	relative	996:1003	arg1	quantitation					1018:1029	relative and absolute quantitation	996:1029	relative and absolute quantitation (iTRAQ)	996:1037	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	2	59	gly	glycosylation	350:362	arg1	receptors					381:389	mannose receptors	373:389	mannose receptors	373:389	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	2	59	gly	glycosylation	350:362	arg2	sites					364:368	several glycosylation sites	342:368	several glycosylation sites of mannose receptors in different breast cancer subtypes	342:425	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	2	59	gly	glycosylation	350:362	arg2	receptors					381:389	mannose receptors	373:389	mannose receptors	373:389	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	5	60	theme	isobaric	978:985	arg1	tags					987:990	isobaric tags	978:990	isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS	978:1050	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	0	61	gly	glycopeptides	31:43	arg2	glycopeptides					31:43	intact glycopeptides	24:43	intact glycopeptides from mannose receptor	24:65	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	0	61	gly	glycopeptides	31:43	arg1	receptor					58:65	mannose receptor	50:65	mannose receptor	50:65	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	4	62	theme	glycosylation	735:747	arg1	site					749:752	the glycosylation site	731:752	the glycosylation site	731:752	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	7	63	gly	glycopeptides	1221:1233	arg2	glycopeptides					1221:1233	11 glycopeptides	1218:1233	11 glycopeptides	1218:1233	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	6	64	theme	breast	1191:1196	arg1	cancer					1198:1203	breast cancer	1191:1203	breast cancer	1191:1203	In total, 7 glycosylation sites and 12 glycan types corresponding to 26 intact glycopeptides were characterized from the four subtypes of breast cancer.					
33303137	7	65	theme	previous	1323:1330	arg1	conclusion					1332:1341	the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes	1319:1446	the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes	1319:1446	Among them, 11 glycopeptides can be used to differentiate the subtypes of breast cancer, which further supported the previous conclusion that mannose receptor can be used as a potential marker for the identification of breast cancer subtypes.					
33303137	2	66	theme	glycosylation	350:362	arg1	sites					364:368	several glycosylation sites	342:368	several glycosylation sites of mannose receptors in different breast cancer subtypes	342:425	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	2	66	theme	glycosylation	350:362	arg1	receptors					381:389	mannose receptors	373:389	mannose receptors	373:389	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	1	67	theme	specific	241:248	arg1	features					261:268	specific pathologic features	241:268	specific pathologic features	241:268	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	0	68	theme	different	73:81	arg1	subtypes					97:104	different breast cancer subtypes	73:104	different breast cancer subtypes	73:104	Comparative analysis of intact glycopeptides from mannose receptor among different breast cancer subtypes using mass spectrometry.					
33303137	4	69	theme	intact	647:652	arg1	glycopeptides					654:666	intact glycopeptides	647:666	intact glycopeptides	647:666	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	4	70	theme	glycosylation	806:818	arg1	difference					792:801	the difference	788:801	the difference of glycosylation in the four subtypes of breast cancer	788:856	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	2	71	theme	breast	404:409	arg1	subtypes					418:425	different breast cancer subtypes	394:425	different breast cancer subtypes	394:425	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	1	72	theme	pathologic	250:259	arg1	features					261:268	specific pathologic features	241:268	specific pathologic features	241:268	Breast cancer is a highly heterogeneous disease, encompassing a number of biologically distinct entities with specific pathologic features and biological behaviors.					
33303137	3	73	theme	previous	620:627	arg1	work					629:632	previous work	620:632	previous work	620:632	However, the glycan composition on each site is still unknown because the glycan was removed by PNGase F in previous work.					
33303137	4	74	from	difference	792:801	arg1	subtypes					832:839	the four subtypes	823:839	the four subtypes of breast cancer	823:856	Analysis of intact glycopeptides can provide the information of both the glycan composition and the glycosylation site, which can further help to reveal the difference of glycosylation in the four subtypes of breast cancer.					
33303137	2	75	theme	different	394:402	arg1	subtypes					418:425	different breast cancer subtypes	394:425	different breast cancer subtypes	394:425	In the preliminary experiments, we identified several glycosylation sites of mannose receptors in different breast cancer subtypes and showed that the mannose receptors could be a potential marker for breast cancer.					
33303137	5	76	from	patients	963:970	arg1	serum					938:942	serum	938:942	serum from breast cancer patients	938:970	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
33303137	5	76	from	patients	963:970	arg1	glycopeptides					896:908	the intact glycopeptides	885:908	the intact glycopeptides of the mannose receptors in serum from breast cancer patients	885:970	In this work, we analyzed the intact glycopeptides of the mannose receptors in serum from breast cancer patients using isobaric tags for relative and absolute quantitation (iTRAQ) and LC-MS/MS.					
34583196	0	0	theme	lacking	92:98	arg1	matriglycans					100:111	α-dystroglycan lacking matriglycans	77:111	α-dystroglycan lacking matriglycans	77:111	Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans.					
34583196	3	1	theme	colon	704:708	arg1	HCT116 cells					720:731	human colon carcinoma HCT116 cells	698:731	human colon carcinoma HCT116 cells	698:731	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	7	2	theme	α-DG	1513:1516	arg1	matriglycans					1526:1537	α-DG lacking matriglycans	1513:1537	α-DG lacking matriglycans	1513:1537	Collectively, our results indicate that DG2 could serve as a useful tool to determine tissue distribution and function of α-DG lacking matriglycans under physiological and pathophysiological conditions.					
34583196	0	3	theme	α-dystroglycan	77:90	arg1	matriglycans					100:111	α-dystroglycan lacking matriglycans	77:111	α-dystroglycan lacking matriglycans	77:111	Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans.					
34583196	3	4	theme	CDP-Gro	687:693	arg1	overproduction					669:682	the overproduction	665:682	the overproduction of CDP-Gro in human colon carcinoma HCT116 cells	665:731	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	6	5	from	expression	1279:1288	arg1	HCT116 cells					1301:1312	HCT116 cells	1301:1312	HCT116 cells	1301:1312	Accordingly, forced expression of TagD in HCT116 cells resulted in the reduction of matriglycans and an increase in DG2 reactivity.					
34583196	4	6	contain	contained	874:882	arg1	α-DG					822:825	α-DG	822:825	α-DG prepared from the TagD-expressing HCT116 cells	822:872	Western blot and liquid chromatography-tandem mass spectrometry analyses indicated that α-DG prepared from the TagD-expressing HCT116 cells contained abundant GroP and lacked matriglycans.					
34583196	4	6	contain	contained	874:882	arg2	GroP					893:896	abundant GroP	884:896	abundant GroP	884:896	Western blot and liquid chromatography-tandem mass spectrometry analyses indicated that α-DG prepared from the TagD-expressing HCT116 cells contained abundant GroP and lacked matriglycans.					
34583196	5	7	theme	knockout	1197:1204	arg1	cells					1206:1210	knockout cells	1197:1210	knockout cells	1197:1210	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	5	8	theme	various	1180:1186	arg1	types					1188:1192	various types	1180:1192	various types of knockout cells deficient in the biosynthesis of matriglycans	1180:1256	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	0	9	gly	glycoforms	63:72	arg1	matriglycans					100:111	α-dystroglycan lacking matriglycans	77:111	α-dystroglycan lacking matriglycans	77:111	Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans.					
34583196	4	10	theme	liquid	751:756	arg1	spectrometry					785:796	liquid chromatography-tandem mass spectrometry	751:796	liquid chromatography-tandem mass spectrometry	751:796	Western blot and liquid chromatography-tandem mass spectrometry analyses indicated that α-DG prepared from the TagD-expressing HCT116 cells contained abundant GroP and lacked matriglycans.					
34583196	1	11	theme	glucuronic	285:294	arg1	acid					296:299	glucuronic acid	285:299	glucuronic acid	285:299	α-Dystroglycan (α-DG) is a glycoprotein specifically modified with O-mannosyl glycans bearing long polysaccharides, termed matriglycans, which comprise repeating units of glucuronic acid and xylose.					
34583196	0	12	theme	matriglycans	100:111	arg1	glycoforms					63:72	truncated glycoforms	53:72	truncated glycoforms of α-dystroglycan lacking matriglycans	53:111	Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans.					
34583196	7	13	theme	lacking	1518:1524	arg1	matriglycans					1526:1537	α-DG lacking matriglycans	1513:1537	α-DG lacking matriglycans	1513:1537	Collectively, our results indicate that DG2 could serve as a useful tool to determine tissue distribution and function of α-DG lacking matriglycans under physiological and pathophysiological conditions.					
34583196	5	14	from	deficient	1212:1220	arg1	biosynthesis					1229:1240	the biosynthesis	1225:1240	the biosynthesis of matriglycans	1225:1256	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	6	15	theme	forced	1272:1277	arg1	expression					1279:1288	forced expression	1272:1288	forced expression of TagD in HCT116 cells	1272:1312	Accordingly, forced expression of TagD in HCT116 cells resulted in the reduction of matriglycans and an increase in DG2 reactivity.					
34583196	4	16	theme	mass	780:783	arg1	spectrometry					785:796	liquid chromatography-tandem mass spectrometry	751:796	liquid chromatography-tandem mass spectrometry	751:796	Western blot and liquid chromatography-tandem mass spectrometry analyses indicated that α-DG prepared from the TagD-expressing HCT116 cells contained abundant GroP and lacked matriglycans.					
34583196	1	17	theme	acid	296:299	arg1	units					276:280	repeating units	266:280	repeating units of glucuronic acid and xylose	266:310	α-Dystroglycan (α-DG) is a glycoprotein specifically modified with O-mannosyl glycans bearing long polysaccharides, termed matriglycans, which comprise repeating units of glucuronic acid and xylose.					
34583196	7	18	theme	matriglycans	1526:1537	arg1	distribution					1484:1495	tissue distribution	1477:1495	tissue distribution	1477:1495	Collectively, our results indicate that DG2 could serve as a useful tool to determine tissue distribution and function of α-DG lacking matriglycans under physiological and pathophysiological conditions.					
34583196	7	18	theme	matriglycans	1526:1537	arg1	function					1501:1508	function	1501:1508	function	1501:1508	Collectively, our results indicate that DG2 could serve as a useful tool to determine tissue distribution and function of α-DG lacking matriglycans under physiological and pathophysiological conditions.					
34583196	7	19	theme	tissue	1477:1482	arg1	distribution					1484:1495	tissue distribution	1477:1495	tissue distribution	1477:1495	Collectively, our results indicate that DG2 could serve as a useful tool to determine tissue distribution and function of α-DG lacking matriglycans under physiological and pathophysiological conditions.					
34583196	1	20	theme	O-mannosyl	181:190	arg1	glycans					192:198	O-mannosyl glycans	181:198	O-mannosyl glycans bearing long polysaccharides, termed matriglycans, which comprise repeating units of glucuronic acid and xylose	181:310	α-Dystroglycan (α-DG) is a glycoprotein specifically modified with O-mannosyl glycans bearing long polysaccharides, termed matriglycans, which comprise repeating units of glucuronic acid and xylose.					
34583196	3	21	theme	carcinoma	710:718	arg1	HCT116 cells					720:731	human colon carcinoma HCT116 cells	698:731	human colon carcinoma HCT116 cells	698:731	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	4	22	theme	abundant	884:891	arg1	GroP					893:896	abundant GroP	884:896	abundant GroP	884:896	Western blot and liquid chromatography-tandem mass spectrometry analyses indicated that α-DG prepared from the TagD-expressing HCT116 cells contained abundant GroP and lacked matriglycans.					
34583196	5	23	theme	monoclonal	991:1000	arg1	antibody					1002:1009	a novel monoclonal antibody	983:1009	a novel monoclonal antibody	983:1009	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	2	24	theme	fukutin-related	490:504	arg1	protein					506:512	fukutin-related protein	490:512	fukutin-related protein	490:512	The matriglycan is linked to the O-mannosyl glycan core through two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro.					
34583196	5	25	from	biosynthesis	1229:1240	arg1	deficient					1212:1220	deficient	1212:1220	deficient	1212:1220	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	3	26	from	overproduction	669:682	arg1	HCT116 cells					720:731	human colon carcinoma HCT116 cells	698:731	human colon carcinoma HCT116 cells	698:731	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	6	27	theme	DG2	1375:1377	arg1	reactivity					1379:1388	DG2 reactivity	1375:1388	DG2 reactivity	1375:1388	Accordingly, forced expression of TagD in HCT116 cells resulted in the reduction of matriglycans and an increase in DG2 reactivity.					
34583196	2	28	attach	linked	332:337	arg1	core					364:367	the O-mannosyl glycan core	342:367	the O-mannosyl glycan core	342:367	The matriglycan is linked to the O-mannosyl glycan core through two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro.					
34583196	2	28	attach	linked	332:337	arg2	matriglycan					317:327	The matriglycan	313:327	The matriglycan	313:327	The matriglycan is linked to the O-mannosyl glycan core through two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro.					
34583196	6	29	from	reduction	1330:1338	arg1	reactivity					1379:1388	DG2 reactivity	1375:1388	DG2 reactivity	1375:1388	Accordingly, forced expression of TagD in HCT116 cells resulted in the reduction of matriglycans and an increase in DG2 reactivity.					
34583196	7	30	theme	pathophysiological	1563:1580	arg1	conditions					1582:1591	physiological and pathophysiological conditions	1545:1591	physiological and pathophysiological conditions	1545:1591	Collectively, our results indicate that DG2 could serve as a useful tool to determine tissue distribution and function of α-DG lacking matriglycans under physiological and pathophysiological conditions.					
34583196	1	31	theme	long	208:211	arg1	polysaccharides					213:227	long polysaccharides	208:227	long polysaccharides	208:227	α-Dystroglycan (α-DG) is a glycoprotein specifically modified with O-mannosyl glycans bearing long polysaccharides, termed matriglycans, which comprise repeating units of glucuronic acid and xylose.					
34583196	0	32	theme	novel	19:23	arg1	antibody					36:43	a novel monoclonal antibody	17:43	a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans	17:111	Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans.					
34583196	1	33	theme	xylose	305:310	arg1	units					276:280	repeating units	266:280	repeating units of glucuronic acid and xylose	266:310	α-Dystroglycan (α-DG) is a glycoprotein specifically modified with O-mannosyl glycans bearing long polysaccharides, termed matriglycans, which comprise repeating units of glucuronic acid and xylose.					
34583196	5	34	theme	novel	985:989	arg1	antibody					1002:1009	a novel monoclonal antibody	983:1009	a novel monoclonal antibody	983:1009	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	4	35	theme	chromatography-tandem	758:778	arg1	spectrometry					785:796	liquid chromatography-tandem mass spectrometry	751:796	liquid chromatography-tandem mass spectrometry	751:796	Western blot and liquid chromatography-tandem mass spectrometry analyses indicated that α-DG prepared from the TagD-expressing HCT116 cells contained abundant GroP and lacked matriglycans.					
34583196	4	36	theme	TagD-expressing	845:859	arg1	HCT116 cells					861:872	the TagD-expressing HCT116 cells	841:872	the TagD-expressing HCT116 cells	841:872	Western blot and liquid chromatography-tandem mass spectrometry analyses indicated that α-DG prepared from the TagD-expressing HCT116 cells contained abundant GroP and lacked matriglycans.					
34583196	2	37	theme	phosphate	440:448	arg1	units					457:461	glycerol phosphate (GroP) units	431:461	glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro	431:544	The matriglycan is linked to the O-mannosyl glycan core through two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro.					
34583196	5	38	theme	matriglycans	1245:1256	arg1	biosynthesis					1229:1240	the biosynthesis	1225:1240	the biosynthesis of matriglycans	1225:1256	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	3	39	theme	forced	567:572	arg1	expression					574:583	forced expression	567:583	forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis	567:647	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	2	40	theme	glycerol	431:438	arg1	GroP					451:454	GroP	451:454	GroP	451:454	The matriglycan is linked to the O-mannosyl glycan core through two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro.					
34583196	2	40	theme	glycerol	431:438	arg1	phosphate					440:448	glycerol phosphate	431:448	glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro	431:544	The matriglycan is linked to the O-mannosyl glycan core through two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro.					
34583196	0	41	theme	antibody	36:43	arg1	Establishment					0:12	Establishment	0:12	Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans.	0:112	Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans.					
34583196	3	42	theme	CDP-Gro	602:608	arg1	TagD					620:623	TagD	620:623	TagD	620:623	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	3	42	theme	CDP-Gro	602:608	arg1	synthase					610:617	the bacterial CDP-Gro synthase	588:617	the bacterial CDP-Gro synthase	588:617	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	0	43	theme	monoclonal	25:34	arg1	antibody					36:43	a novel monoclonal antibody	17:43	a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans	17:111	Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans.					
34583196	5	44	theme	cells	1206:1210	arg1	types					1188:1192	various types	1180:1192	various types of knockout cells deficient in the biosynthesis of matriglycans	1180:1256	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	3	45	theme	synthase	610:617	arg1	expression					574:583	forced expression	567:583	forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis	567:647	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	0	46	theme	truncated	53:61	arg1	glycoforms					63:72	truncated glycoforms	53:72	truncated glycoforms of α-dystroglycan lacking matriglycans	53:111	Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans.					
34583196	5	47	theme	GroP-containing	933:947	arg1	α-DG-Fc					961:967	the GroP-containing recombinant α-DG-Fc	929:967	the GroP-containing recombinant α-DG-Fc	929:967	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	6	48	theme	TagD	1293:1296	arg1	expression					1279:1288	forced expression	1272:1288	forced expression of TagD in HCT116 cells	1272:1312	Accordingly, forced expression of TagD in HCT116 cells resulted in the reduction of matriglycans and an increase in DG2 reactivity.					
34583196	5	49	theme	DG2	1168:1170	arg1	reactivity					1154:1163	the reactivity	1150:1163	the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans	1150:1256	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	6	50	from	increase	1363:1370	arg1	reactivity					1379:1388	DG2 reactivity	1375:1388	DG2 reactivity	1375:1388	Accordingly, forced expression of TagD in HCT116 cells resulted in the reduction of matriglycans and an increase in DG2 reactivity.					
34583196	7	51	theme	physiological	1545:1557	arg1	conditions					1582:1591	physiological and pathophysiological conditions	1545:1591	physiological and pathophysiological conditions	1545:1591	Collectively, our results indicate that DG2 could serve as a useful tool to determine tissue distribution and function of α-DG lacking matriglycans under physiological and pathophysiological conditions.					
34583196	1	52	gly	glycoprotein	141:152	arg1	α-Dystroglycan					114:127	α-Dystroglycan	114:127	α-Dystroglycan (α-DG)	114:134	α-Dystroglycan (α-DG) is a glycoprotein specifically modified with O-mannosyl glycans bearing long polysaccharides, termed matriglycans, which comprise repeating units of glucuronic acid and xylose.					
34583196	1	52	gly	glycoprotein	141:152	arg1	glycoprotein					141:152	a glycoprotein	139:152	a glycoprotein specifically modified with O-mannosyl glycans bearing long polysaccharides, termed matriglycans, which comprise repeating units of glucuronic acid and xylose	139:310	α-Dystroglycan (α-DG) is a glycoprotein specifically modified with O-mannosyl glycans bearing long polysaccharides, termed matriglycans, which comprise repeating units of glucuronic acid and xylose.					
34583196	5	53	theme	GroP-terminated	1089:1103	arg1	glycoforms					1105:1114	GroP-terminated glycoforms	1089:1114	GroP-terminated glycoforms lacking matriglycans	1089:1135	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	3	54	theme	bacterial	592:600	arg1	TagD					620:623	TagD	620:623	TagD	620:623	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	3	54	theme	bacterial	592:600	arg1	synthase					610:617	the bacterial CDP-Gro synthase	588:617	the bacterial CDP-Gro synthase	588:617	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	2	55	theme	glycan	357:362	arg1	core					364:367	the O-mannosyl glycan core	342:367	the O-mannosyl glycan core	342:367	The matriglycan is linked to the O-mannosyl glycan core through two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro.					
34583196	5	56	theme	α-DG	1073:1076	arg1	glycoforms					1059:1068	several truncated glycoforms	1041:1068	several truncated glycoforms	1041:1068	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	5	56	theme	α-DG	1073:1076	arg1	glycoforms					1105:1114	GroP-terminated glycoforms	1089:1114	GroP-terminated glycoforms lacking matriglycans	1089:1135	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	2	57	theme	O-mannosyl	346:355	arg1	core					364:367	the O-mannosyl glycan core	342:367	the O-mannosyl glycan core	342:367	The matriglycan is linked to the O-mannosyl glycan core through two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro.					
34583196	7	58	theme	useful	1452:1457	arg1	DG2					1431:1433	DG2	1431:1433	DG2	1431:1433	Collectively, our results indicate that DG2 could serve as a useful tool to determine tissue distribution and function of α-DG lacking matriglycans under physiological and pathophysiological conditions.					
34583196	7	58	theme	useful	1452:1457	arg1	tool					1459:1462	a useful tool	1450:1462	a useful tool to determine tissue distribution and function of α-DG lacking matriglycans under physiological and pathophysiological conditions	1450:1591	Collectively, our results indicate that DG2 could serve as a useful tool to determine tissue distribution and function of α-DG lacking matriglycans under physiological and pathophysiological conditions.					
34583196	5	59	theme	several	1041:1047	arg1	glycoforms					1059:1068	several truncated glycoforms	1041:1068	several truncated glycoforms	1041:1068	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	5	59	theme	several	1041:1047	arg1	glycoforms					1105:1114	GroP-terminated glycoforms	1089:1114	GroP-terminated glycoforms lacking matriglycans	1089:1135	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	4	60	dep	blot	742:745	arg1	analyses					798:805	analyses	798:805	analyses	798:805	Western blot and liquid chromatography-tandem mass spectrometry analyses indicated that α-DG prepared from the TagD-expressing HCT116 cells contained abundant GroP and lacked matriglycans.					
34583196	4	61	theme	Western	734:740	arg1	blot					742:745	Western blot	734:745	Western blot	734:745	Western blot and liquid chromatography-tandem mass spectrometry analyses indicated that α-DG prepared from the TagD-expressing HCT116 cells contained abundant GroP and lacked matriglycans.					
34583196	5	62	theme	deficient	1212:1220	arg1	types					1188:1192	various types	1180:1192	various types of knockout cells deficient in the biosynthesis of matriglycans	1180:1256	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	5	63	theme	recombinant	949:959	arg1	α-DG-Fc					961:967	the GroP-containing recombinant α-DG-Fc	929:967	the GroP-containing recombinant α-DG-Fc	929:967	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	5	64	theme	truncated	1049:1057	arg1	glycoforms					1059:1068	several truncated glycoforms	1041:1068	several truncated glycoforms	1041:1068	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	5	64	theme	truncated	1049:1057	arg1	glycoforms					1105:1114	GroP-terminated glycoforms	1089:1114	GroP-terminated glycoforms lacking matriglycans	1089:1135	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	2	65	theme	phosphate	389:397	arg1	units					399:403	two ribitol phosphate units	377:403	two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro	377:544	The matriglycan is linked to the O-mannosyl glycan core through two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro.					
34583196	5	66	gly	glycoforms	1059:1068	arg1	α-DG					1073:1076	α-DG	1073:1076	α-DG	1073:1076	Using the GroP-containing recombinant α-DG-Fc, we developed a novel monoclonal antibody, termed DG2, that reacts with several truncated glycoforms of α-DG, including GroP-terminated glycoforms lacking matriglycans; we verified the reactivity of DG2 against various types of knockout cells deficient in the biosynthesis of matriglycans.					
34583196	3	67	theme	human	698:702	arg1	HCT116 cells					720:731	human colon carcinoma HCT116 cells	698:731	human colon carcinoma HCT116 cells	698:731	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	3	68	from	subtilis	640:647	arg1	expression					574:583	forced expression	567:583	forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis	567:647	Here, we found that forced expression of the bacterial CDP-Gro synthase, TagD, from Bacillus subtilis could result in the overproduction of CDP-Gro in human colon carcinoma HCT116 cells.					
34583196	2	69	theme	ribitol	381:387	arg1	units					399:403	two ribitol phosphate units	377:403	two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro	377:544	The matriglycan is linked to the O-mannosyl glycan core through two ribitol phosphate units that can be replaced with glycerol phosphate (GroP) units synthesized by fukutin and fukutin-related protein that transfer GroP from CDP-Gro.					
34583196	6	70	theme	matriglycans	1343:1354	arg1	increase					1363:1370	an increase	1360:1370	an increase in DG2 reactivity	1360:1388	Accordingly, forced expression of TagD in HCT116 cells resulted in the reduction of matriglycans and an increase in DG2 reactivity.					
34583196	6	70	theme	matriglycans	1343:1354	arg1	reduction					1330:1338	the reduction	1326:1338	the reduction of matriglycans	1326:1354	Accordingly, forced expression of TagD in HCT116 cells resulted in the reduction of matriglycans and an increase in DG2 reactivity.					
34583196	1	71	theme	repeating	266:274	arg1	units					276:280	repeating units	266:280	repeating units of glucuronic acid and xylose	266:310	α-Dystroglycan (α-DG) is a glycoprotein specifically modified with O-mannosyl glycans bearing long polysaccharides, termed matriglycans, which comprise repeating units of glucuronic acid and xylose.					
33188815	4	0	theme	P.	665:666	arg1	boryana					668:674	P. boryana	665:674	P. boryana	665:674	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	9	1	theme	polysaccharides	1600:1614	arg1	properties					1560:1569	properties	1560:1569	properties	1560:1569	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	9	1	theme	polysaccharides	1600:1614	arg1	bioactivities					1575:1587	bioactivities	1575:1587	bioactivities	1575:1587	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	2	2	theme	seaweed	358:364	arg1	industry					366:373	the seaweed industry	354:373	the seaweed industry	354:373	However, Malaysian seaweeds are under-utilised, leading to low revenue throughout the supply chain of the seaweed industry.					
33188815	8	3	theme	highest	1437:1443	arg1	activity					1472:1479	the significantly highest (p ≤ 0.05) DPPH scavenging activity	1419:1479	the significantly highest (p ≤ 0.05) DPPH scavenging activity	1419:1479	On the other hand, the significantly highest (p ≤ 0.05) DPPH scavenging activity was recorded in alginate with Asar at 85.3 ± 0.8%.					
33188815	0	4	theme	brown	78:82	arg1	seaweeds					84:91	Malaysian brown seaweeds	68:91	Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana)	68:152	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).					
33188815	3	5	theme	extracted	638:646	arg1	polysaccharides					648:662	the extracted polysaccharides	634:662	the extracted polysaccharides	634:662	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	1	6	theme	Brown	155:159	arg1	seaweeds					161:168	Brown seaweeds	155:168	Brown seaweeds	155:168	Brown seaweeds are rich source of functional polysaccharides that exhibit various bioactivities.					
33188815	1	6	theme	Brown	155:159	arg1	source					179:184	rich source	174:184	rich source of functional polysaccharides that exhibit various bioactivities	174:249	Brown seaweeds are rich source of functional polysaccharides that exhibit various bioactivities.					
33188815	9	7	from	seaweeds	1740:1747	arg1	ingredients					1707:1717	high-value functional ingredients	1685:1717	high-value functional ingredients from Malaysian brown seaweeds	1685:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	7	8	dep	highest	1264:1270	arg1	total					1283:1287	total	1283:1287	total	1283:1287	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	3	9	theme	polysaccharides	648:662	arg1	properties					620:629	the properties	616:629	the properties of the extracted polysaccharides	616:662	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	6	10	theme	spectral	1150:1157	arg1	pattern					1159:1165	similar spectral pattern	1142:1165	similar spectral pattern of spectra	1142:1176	Fourier transform infrared (FTIR) spectroscopy analysis demonstrated that the extracted polysaccharides exhibited similar spectral pattern of spectra with the respective standards.					
33188815	6	11	theme	spectra	1170:1176	arg1	pattern					1159:1165	similar spectral pattern	1142:1165	similar spectral pattern of spectra	1142:1176	Fourier transform infrared (FTIR) spectroscopy analysis demonstrated that the extracted polysaccharides exhibited similar spectral pattern of spectra with the respective standards.					
33188815	4	12	contain	contained	825:833	arg2	%					904:904	Atur = 105.19 ± 3.45%	884:904	Atur = 105.19 ± 3.45%	884:904	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	12	contain	contained	825:833	arg2	yield					877:881	alginate yield	868:881	highest alginate yield (Atur = 105.19 ± 3.45%)	860:905	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	12	contain	contained	825:833	arg1	ornata					818:823	T. ornata	815:823	T. ornata	815:823	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	6	13	theme	similar	1142:1148	arg1	pattern					1159:1165	similar spectral pattern	1142:1165	similar spectral pattern of spectra	1142:1176	Fourier transform infrared (FTIR) spectroscopy analysis demonstrated that the extracted polysaccharides exhibited similar spectral pattern of spectra with the respective standards.					
33188815	4	14	theme	carbohydrate	722:733	arg1	content					735:741	the significantly (p ≤ 0.05) highest carbohydrate content	685:741	the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%)	685:807	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	14	theme	carbohydrate	722:733	arg1	%					756:756	74.78 ± 1.63%	744:756	74.78 ± 1.63%	744:756	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	3	15	dep	seaweeds	520:527	arg1	polycystum					540:549	Sargassum polycystum	530:549	Sargassum polycystum	530:549	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	3	15	dep	seaweeds	520:527	arg1	ornata					563:568	Turbinaria ornata	552:568	Turbinaria ornata	552:568	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	3	15	dep	seaweeds	520:527	arg1	boryana					581:587	Padina boryana	574:587	Padina boryana	574:587	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	9	16	from	seaweeds	1637:1644	arg1	polysaccharides					1600:1614	natural polysaccharides	1592:1614	natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds	1592:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	9	16	from	seaweeds	1637:1644	arg1	properties					1560:1569	properties	1560:1569	properties	1560:1569	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	9	16	from	seaweeds	1637:1644	arg1	bioactivities					1575:1587	bioactivities	1575:1587	bioactivities	1575:1587	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	9	17	from	bioactivities	1575:1587	arg1	seaweeds					1637:1644	Malaysian brown seaweeds	1621:1644	Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds	1621:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	5	18	theme	extracted	934:942	arg1	polysaccharides					944:958	these extracted polysaccharides	928:958	these extracted polysaccharides	928:958	Water activities of these extracted polysaccharides varied from 0.63-0.71 with average score of browning indexes (~40).					
33188815	6	19	theme	respective	1187:1196	arg1	standards					1198:1206	the respective standards	1183:1206	the respective standards	1183:1206	Fourier transform infrared (FTIR) spectroscopy analysis demonstrated that the extracted polysaccharides exhibited similar spectral pattern of spectra with the respective standards.					
33188815	9	20	theme	high-value	1685:1694	arg1	ingredients					1707:1717	high-value functional ingredients	1685:1717	high-value functional ingredients from Malaysian brown seaweeds	1685:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	5	21	theme	polysaccharides	944:958	arg1	activities					914:923	Water activities	908:923	Water activities of these extracted polysaccharides	908:958	Water activities of these extracted polysaccharides varied from 0.63-0.71 with average score of browning indexes (~40).					
33188815	7	22	theme	Meanwhile	1209:1217	arg1	extracts					1230:1237	Meanwhile, laminaran extracts	1209:1237	Meanwhile, laminaran extracts	1209:1237	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	9	23	theme	functional	1696:1705	arg1	ingredients					1707:1717	high-value functional ingredients	1685:1717	high-value functional ingredients from Malaysian brown seaweeds	1685:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	0	24	dep	seaweeds	84:91	arg1	polycystum					104:113	Sargassum polycystum	94:113	Sargassum polycystum	94:113	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).					
33188815	0	24	dep	seaweeds	84:91	arg1	ornata					127:132	Turbinaria ornata	116:132	Turbinaria ornata	116:132	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).					
33188815	0	24	dep	seaweeds	84:91	arg1	boryana					145:151	Padina boryana	138:151	Padina boryana	138:151	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).					
33188815	9	25	dep	properties	1560:1569	arg1	the					1556:1558	the	1556:1558	the	1556:1558	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	1	26	theme	rich	174:177	arg1	seaweeds					161:168	Brown seaweeds	155:168	Brown seaweeds	155:168	Brown seaweeds are rich source of functional polysaccharides that exhibit various bioactivities.					
33188815	1	26	theme	rich	174:177	arg1	source					179:184	rich source	174:184	rich source of functional polysaccharides that exhibit various bioactivities	174:249	Brown seaweeds are rich source of functional polysaccharides that exhibit various bioactivities.					
33188815	0	27	theme	Functional	0:9	arg1	polysaccharides					11:25	Functional polysaccharides	0:25	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).	0:153	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).					
33188815	9	28	theme	brown	1631:1635	arg1	seaweeds					1637:1644	Malaysian brown seaweeds	1621:1644	Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds	1621:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	8	29	theme	scavenging	1461:1470	arg1	activity					1472:1479	the significantly highest (p ≤ 0.05) DPPH scavenging activity	1419:1479	the significantly highest (p ≤ 0.05) DPPH scavenging activity	1419:1479	On the other hand, the significantly highest (p ≤ 0.05) DPPH scavenging activity was recorded in alginate with Asar at 85.3 ± 0.8%.					
33188815	3	30	theme	functional	419:428	arg1	polysaccharides					430:444	the functional polysaccharides	415:444	the functional polysaccharides	415:444	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	3	30	theme	functional	419:428	arg1	laminaran					468:476	laminaran	468:476	laminaran (L)	468:480	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	3	30	theme	functional	419:428	arg1	fucoidan					454:461	fucoidan	454:461	fucoidan (F)	454:465	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	3	30	theme	functional	419:428	arg1	alginate					486:493	alginate	486:493	alginate (A)	486:497	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	0	31	theme	fucoidan	30:37	arg1	polysaccharides					11:25	Functional polysaccharides	0:25	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).	0:153	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).					
33188815	7	32	theme	superoxide	1341:1350	arg1	anion					1352:1356	superoxide anion	1341:1356	superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%)	1341:1397	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	3	33	theme	Malaysian	504:512	arg1	seaweeds					520:527	Malaysian brown seaweeds	504:527	Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana)	504:588	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	9	34	theme	Malaysian	1621:1629	arg1	seaweeds					1637:1644	Malaysian brown seaweeds	1621:1644	Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds	1621:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	4	35	with	content	735:741	arg1	yield					781:785	highest fucoidan yield	764:785	highest fucoidan yield (Fpad = 1.59 ± 0.16%)	764:807	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	35	with	content	735:741	arg1	%					806:806	Fpad = 1.59 ± 0.16%	788:806	Fpad = 1.59 ± 0.16%	788:806	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	7	36	dep	Meanwhile	1209:1217	arg1	laminaran					1220:1228	laminaran	1220:1228	laminaran	1220:1228	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	0	37	from	seaweeds	84:91	arg1	polysaccharides					11:25	Functional polysaccharides	0:25	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).	0:153	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).					
33188815	3	38	theme	brown	514:518	arg1	seaweeds					520:527	Malaysian brown seaweeds	504:527	Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana)	504:588	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	1	39	theme	functional	189:198	arg1	polysaccharides					200:214	functional polysaccharides	189:214	functional polysaccharides that exhibit various bioactivities	189:249	Brown seaweeds are rich source of functional polysaccharides that exhibit various bioactivities.					
33188815	0	40	theme	laminaran	40:48	arg1	polysaccharides					11:25	Functional polysaccharides	0:25	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).	0:153	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).					
33188815	9	41	theme	brown	1734:1738	arg1	seaweeds					1740:1747	Malaysian brown seaweeds	1724:1747	Malaysian brown seaweeds	1724:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	5	42	dep	varied	960:965	arg1	~40					1022:1024	~40	1022:1024	~40	1022:1024	Water activities of these extracted polysaccharides varied from 0.63-0.71 with average score of browning indexes (~40).					
33188815	5	43	theme	average	987:993	arg1	score					995:999	average score	987:999	average score of browning indexes	987:1019	Water activities of these extracted polysaccharides varied from 0.63-0.71 with average score of browning indexes (~40).					
33188815	1	44	theme	polysaccharides	200:214	arg1	seaweeds					161:168	Brown seaweeds	155:168	Brown seaweeds	155:168	Brown seaweeds are rich source of functional polysaccharides that exhibit various bioactivities.					
33188815	1	44	theme	polysaccharides	200:214	arg1	source					179:184	rich source	174:184	rich source of functional polysaccharides that exhibit various bioactivities	174:249	Brown seaweeds are rich source of functional polysaccharides that exhibit various bioactivities.					
33188815	4	45	dep	significantly	835:847	arg1	p ≤ 0.05					850:857	p ≤ 0.05	850:857	p ≤ 0.05	850:857	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	46	theme	fucoidan	772:779	arg1	yield					781:785	highest fucoidan yield	764:785	highest fucoidan yield (Fpad = 1.59 ± 0.16%)	764:807	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	46	theme	fucoidan	772:779	arg1	%					806:806	Fpad = 1.59 ± 0.16%	788:806	Fpad = 1.59 ± 0.16%	788:806	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	47	theme	highest	714:720	arg1	content					735:741	the significantly (p ≤ 0.05) highest carbohydrate content	685:741	the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%)	685:807	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	47	theme	highest	714:720	arg1	%					756:756	74.78 ± 1.63%	744:756	74.78 ± 1.63%	744:756	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	0	48	theme	alginate	54:61	arg1	polysaccharides					11:25	Functional polysaccharides	0:25	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).	0:153	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).					
33188815	6	49	dep	infrared	1046:1053	arg1	FTIR					1056:1059	FTIR	1056:1059	FTIR	1056:1059	Fourier transform infrared (FTIR) spectroscopy analysis demonstrated that the extracted polysaccharides exhibited similar spectral pattern of spectra with the respective standards.					
33188815	8	50	theme	other	1407:1411	arg1	hand					1413:1416	the other hand	1403:1416	the other hand	1403:1416	On the other hand, the significantly highest (p ≤ 0.05) DPPH scavenging activity was recorded in alginate with Asar at 85.3 ± 0.8%.					
33188815	4	51	dep	highest	714:720	arg1	p ≤ 0.05					704:711	p ≤ 0.05	704:711	p ≤ 0.05	704:711	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	5	52	theme	Water	908:912	arg1	activities					914:923	Water activities	908:923	Water activities of these extracted polysaccharides	908:958	Water activities of these extracted polysaccharides varied from 0.63-0.71 with average score of browning indexes (~40).					
33188815	5	53	theme	indexes	1013:1019	arg1	score					995:999	average score	987:999	average score of browning indexes	987:1019	Water activities of these extracted polysaccharides varied from 0.63-0.71 with average score of browning indexes (~40).					
33188815	5	54	theme	browning	1004:1011	arg1	indexes					1013:1019	browning indexes	1004:1019	browning indexes	1004:1019	Water activities of these extracted polysaccharides varied from 0.63-0.71 with average score of browning indexes (~40).					
33188815	4	55	theme	alginate	868:875	arg1	yield					877:881	alginate yield	868:881	highest alginate yield (Atur = 105.19 ± 3.45%)	860:905	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	55	theme	alginate	868:875	arg1	%					904:904	Atur = 105.19 ± 3.45%	884:904	Atur = 105.19 ± 3.45%	884:904	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	8	56	theme	DPPH	1456:1459	arg1	activity					1472:1479	the significantly highest (p ≤ 0.05) DPPH scavenging activity	1419:1479	the significantly highest (p ≤ 0.05) DPPH scavenging activity	1419:1479	On the other hand, the significantly highest (p ≤ 0.05) DPPH scavenging activity was recorded in alginate with Asar at 85.3 ± 0.8%.					
33188815	7	57	theme	scavenging	1358:1367	arg1	activity					1369:1376	superoxide anion scavenging activity	1341:1376	superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%)	1341:1397	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	7	57	theme	scavenging	1358:1367	arg1	%					1396:1396	Lsig = 21.7 ± 3.6%	1379:1396	Lsig = 21.7 ± 3.6%	1379:1396	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	9	58	theme	natural	1592:1598	arg1	polysaccharides					1600:1614	natural polysaccharides	1592:1614	natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds	1592:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	2	59	theme	supply	338:343	arg1	chain					345:349	the supply chain	334:349	the supply chain of the seaweed industry	334:373	However, Malaysian seaweeds are under-utilised, leading to low revenue throughout the supply chain of the seaweed industry.					
33188815	2	60	theme	low	311:313	arg1	revenue					315:321	low revenue	311:321	low revenue throughout the supply chain of the seaweed industry	311:373	However, Malaysian seaweeds are under-utilised, leading to low revenue throughout the supply chain of the seaweed industry.					
33188815	4	61	theme	highest	860:866	arg1	yield					877:881	alginate yield	868:881	highest alginate yield (Atur = 105.19 ± 3.45%)	860:905	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	61	theme	highest	860:866	arg1	%					904:904	Atur = 105.19 ± 3.45%	884:904	Atur = 105.19 ± 3.45%	884:904	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	2	62	theme	Malaysian	261:269	arg1	seaweeds					271:278	Malaysian seaweeds	261:278	Malaysian seaweeds	261:278	However, Malaysian seaweeds are under-utilised, leading to low revenue throughout the supply chain of the seaweed industry.					
33188815	3	63	from	seaweeds	520:527	arg1	extract					407:413	extract	407:413	extract	407:413	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	1	64	theme	various	229:235	arg1	bioactivities					237:249	various bioactivities	229:249	various bioactivities	229:249	Brown seaweeds are rich source of functional polysaccharides that exhibit various bioactivities.					
33188815	0	65	theme	Malaysian	68:76	arg1	seaweeds					84:91	Malaysian brown seaweeds	68:91	Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana)	68:152	Functional polysaccharides of fucoidan, laminaran and alginate from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana).					
33188815	7	66	theme	phenolic	1289:1296	arg1	contents					1298:1305	the significantly highest (p ≤ 0.05) total phenolic contents	1246:1305	the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g)	1246:1335	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	7	66	theme	phenolic	1289:1296	arg1	mgGAE/g					1328:1334	Lsar = 43.29 ± 0.43 mgGAE/g	1308:1334	Lsar = 43.29 ± 0.43 mgGAE/g	1308:1334	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	4	67	theme	highest	764:770	arg1	yield					781:785	highest fucoidan yield	764:785	highest fucoidan yield (Fpad = 1.59 ± 0.16%)	764:807	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	4	67	theme	highest	764:770	arg1	%					806:806	Fpad = 1.59 ± 0.16%	788:806	Fpad = 1.59 ± 0.16%	788:806	P. boryana recorded the significantly (p ≤ 0.05) highest carbohydrate content (74.78 ± 1.63%) with highest fucoidan yield (Fpad = 1.59 ± 0.16%) while T. ornata contained significantly (p ≤ 0.05) highest alginate yield (Atur = 105.19 ± 3.45%).					
33188815	6	68	theme	spectroscopy	1062:1073	arg1	analysis					1075:1082	spectroscopy analysis	1062:1082	spectroscopy analysis	1062:1082	Fourier transform infrared (FTIR) spectroscopy analysis demonstrated that the extracted polysaccharides exhibited similar spectral pattern of spectra with the respective standards.					
33188815	9	69	from	properties	1560:1569	arg1	seaweeds					1637:1644	Malaysian brown seaweeds	1621:1644	Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds	1621:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
33188815	3	70	theme	study	393:397	arg1	aims					380:383	The aims	376:383	The aims of this study	376:397	The aims of this study were to extract the functional polysaccharides, namely fucoidan (F), laminaran (L) and alginate (A) from Malaysian brown seaweeds (Sargassum polycystum, Turbinaria ornata and Padina boryana) and subsequently evaluate the properties of the extracted polysaccharides.					
33188815	6	71	theme	extracted	1106:1114	arg1	polysaccharides					1116:1130	the extracted polysaccharides	1102:1130	the extracted polysaccharides	1102:1130	Fourier transform infrared (FTIR) spectroscopy analysis demonstrated that the extracted polysaccharides exhibited similar spectral pattern of spectra with the respective standards.					
33188815	7	72	theme	anion	1352:1356	arg1	activity					1369:1376	superoxide anion scavenging activity	1341:1376	superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%)	1341:1397	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	7	72	theme	anion	1352:1356	arg1	%					1396:1396	Lsig = 21.7 ± 3.6%	1379:1396	Lsig = 21.7 ± 3.6%	1379:1396	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	7	73	theme	highest	1264:1270	arg1	contents					1298:1305	the significantly highest (p ≤ 0.05) total phenolic contents	1246:1305	the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g)	1246:1335	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	7	73	theme	highest	1264:1270	arg1	mgGAE/g					1328:1334	Lsar = 43.29 ± 0.43 mgGAE/g	1308:1334	Lsar = 43.29 ± 0.43 mgGAE/g	1308:1334	Meanwhile, laminaran extracts showed the significantly highest (p ≤ 0.05) total phenolic contents (Lsar = 43.29 ± 0.43 mgGAE/g) and superoxide anion scavenging activity (Lsig = 21.7 ± 3.6%).					
33188815	2	74	theme	industry	366:373	arg1	chain					345:349	the supply chain	334:349	the supply chain of the seaweed industry	334:373	However, Malaysian seaweeds are under-utilised, leading to low revenue throughout the supply chain of the seaweed industry.					
33188815	9	75	theme	Malaysian	1724:1732	arg1	seaweeds					1740:1747	Malaysian brown seaweeds	1724:1747	Malaysian brown seaweeds	1724:1747	These findings reported the properties and bioactivities of natural polysaccharides from Malaysian brown seaweeds that revealed the potential to develop high-value functional ingredients from Malaysian brown seaweeds.					
32468908	8	0	theme	sidechain	1108:1116	arg1	A					1118:1118	sidechain A	1108:1118	sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts	1108:1186	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	10	1	theme	minimum	1510:1516	arg1	structure					1518:1526	a minimum structure	1508:1526	a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-)	1508:1592	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	1	2	theme	cell	264:267	arg1	walls					269:273	the cell walls	260:273	the cell walls of all vascular plants	260:296	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	10	3	gly	glycoforms	1409:1418	arg1	B					1407:1407	the sidechain B glycoforms	1393:1418	the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots	1393:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	10	3	gly	glycoforms	1409:1418	arg2	RG-II					1423:1427	RG-II	1423:1427	RG-II from different dicots and nonpoalean monocots	1423:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	10	3	gly	glycoforms	1409:1418	arg1	sidechain					1397:1405	the sidechain B glycoforms	1393:1418	the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots	1393:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	8	4	theme	selected	1135:1142	arg1	RG-IIs					1144:1149	selected RG-IIs	1135:1149	selected RG-IIs	1135:1149	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	10	5	theme	O-acetylated	1534:1545	arg1	-Gal-AceA-Rha-Api-					1574:1591	an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-)	1531:1592	an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-)	1531:1592	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	10	6	theme	sidechain	1397:1405	arg1	glycoforms					1409:1418	the sidechain B glycoforms	1393:1418	the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots	1393:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	1	7	theme	pectic	186:191	arg1	polysaccharide					193:206	a structurally complex pectic polysaccharide	163:206	a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants	163:296	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	1	7	theme	pectic	186:191	arg1	Rhamnogalacturonan					130:147	Rhamnogalacturonan II	130:150	Rhamnogalacturonan II (RG-II)	130:158	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	3	8	theme	backbone	563:570	arg1	esterification					528:541	Methyl esterification	521:541	Methyl esterification of the galacturonan backbone	521:570	Methyl esterification of the galacturonan backbone has also been reported but not confirmed.					
32468908	8	9	theme	NaBD4-reduced	1161:1173	arg1	counterparts					1175:1186	their NaBD4-reduced counterparts	1155:1186	their NaBD4-reduced counterparts	1155:1186	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	8	10	theme	GalA	1240:1243	arg1	esterification					1256:1269	methyl esterification	1249:1269	methyl esterification of the GlcA	1249:1281	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	8	10	theme	GalA	1240:1243	arg1	etherification					1209:1222	methyl etherification	1202:1222	methyl etherification of the β-linked GalA	1202:1243	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	8	11	theme	methyl	1249:1254	arg1	esterification					1256:1269	methyl esterification	1249:1269	methyl esterification of the GlcA	1249:1281	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	5	12	theme	-reduced	690:697	arg1	RG-II					699:703	sodium borodeuteride (NaBD4)-reduced RG-II	662:703	sodium borodeuteride (NaBD4)-reduced RG-II	662:703	sodium borodeuteride (NaBD4)-reduced RG-II, to identify the methyl esterification status of backbone GalAs.					
32468908	7	13	theme	3,4-O-methyl	950:961	arg1	GalA					963:966	3-, 4-, and 3,4-O-methyl GalA	938:966	3-, 4-, and 3,4-O-methyl GalA	938:966	We also adapted a procedure based on methanolysis and NaBD4 reduction to identify 3-, 4-, and 3,4-O-methyl GalA in RG-II.					
32468908	1	14	theme	borate	225:230	arg1	dimer					251:255	a borate ester cross-linked dimer	223:255	a borate ester cross-linked dimer	223:255	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	10	15	from	dicots	1444:1449	arg1	RG-II					1423:1427	RG-II	1423:1427	RG-II from different dicots and nonpoalean monocots	1423:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	10	15	from	dicots	1444:1449	arg1	glycoforms					1409:1418	the sidechain B glycoforms	1393:1418	the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots	1393:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	8	16	theme	GlcA	1278:1281	arg1	esterification					1256:1269	methyl esterification	1249:1269	methyl esterification of the GlcA	1249:1281	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	8	16	theme	GlcA	1278:1281	arg1	etherification					1209:1222	methyl etherification	1202:1222	methyl etherification of the β-linked GalA	1202:1243	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	5	17	theme	methyl	722:727	arg1	status					744:749	the methyl esterification status	718:749	the methyl esterification status of backbone GalAs	718:767	sodium borodeuteride (NaBD4)-reduced RG-II, to identify the methyl esterification status of backbone GalAs.					
32468908	0	18	theme	Wall	80:83	arg1	Rhamnogalacturonan					107:124	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	Locating Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II.					
32468908	12	19	theme	dimer	1905:1909	arg1	self-assembly					1911:1923	dimer self-assembly	1905:1923	dimer self-assembly	1905:1923	Taken together, our data further refine the primary sequence and sequence variation of RG-II and provide additional insight into dimer stability and factors controlling dimer self-assembly.					
32468908	2	20	theme	plant	506:510	arg1	species					512:518	plant species	506:518	plant species	506:518	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	10	21	theme	glycoforms	1409:1418	arg1	analysis					1381:1388	Our analysis	1377:1388	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots	1377:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	0	22	theme	Polysaccharide	92:105	arg1	Rhamnogalacturonan					107:124	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	Locating Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II.					
32468908	11	23	theme	further	1635:1641	arg1	evidence					1643:1650	further evidence	1635:1650	further evidence showing that dimer formation and stability in vitro is cation and borate dependent	1635:1733	To complement these studies, we provide further evidence showing that dimer formation and stability in vitro is cation and borate dependent.					
32468908	2	24	theme	acid	392:395	arg1	etherification					411:424	galacturonic acid (GalA) methyl etherification	379:424	galacturonic acid (GalA) methyl etherification	379:424	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	2	25	dep	etherification	411:424	arg1	esterification					460:473	methyl esterification	453:473	methyl esterification	453:473	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	10	26	theme	different	1434:1442	arg1	dicots					1444:1449	different dicots	1434:1449	different dicots	1434:1449	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	8	27	theme	mass	1063:1066	arg1	MALDI-TOF					1082:1090	MALDI-TOF	1082:1090	MALDI-TOF	1082:1090	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	8	27	theme	mass	1063:1066	arg1	spectrometry					1068:1079	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry	1004:1079	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts	1004:1186	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	2	28	theme	methyl	404:409	arg1	etherification					411:424	galacturonic acid (GalA) methyl etherification	379:424	galacturonic acid (GalA) methyl etherification	379:424	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	1	29	theme	plants	291:296	arg1	walls					269:273	the cell walls	260:273	the cell walls of all vascular plants	260:296	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	8	30	theme	matrix-assisted	1004:1018	arg1	MALDI-TOF					1082:1090	MALDI-TOF	1082:1090	MALDI-TOF	1082:1090	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	8	30	theme	matrix-assisted	1004:1018	arg1	spectrometry					1068:1079	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry	1004:1079	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts	1004:1186	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	2	31	theme	methyl	453:458	arg1	esterification					460:473	methyl esterification	453:473	methyl esterification	453:473	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	6	32	dep	two	798:800	arg1	to					795:796	to	795:796	to	795:796	Our data suggest that up to two different GalAs are esterified in the RG-II backbone.					
32468908	2	33	theme	GalA	398:401	arg1	etherification					411:424	galacturonic acid (GalA) methyl etherification	379:424	galacturonic acid (GalA) methyl etherification	379:424	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	0	34	theme	Uronic	49:54	arg1	Acids					56:60	Methyl-Etherified and Methyl-Esterified Uronic Acids	9:60	Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	9:127	Locating Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II.					
32468908	5	35	theme	backbone	754:761	arg1	GalAs					763:767	backbone GalAs	754:767	backbone GalAs	754:767	sodium borodeuteride (NaBD4)-reduced RG-II, to identify the methyl esterification status of backbone GalAs.					
32468908	0	36	theme	Plant	69:73	arg1	Rhamnogalacturonan					107:124	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	Locating Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II.					
32468908	8	37	theme	desorption/ionization-time-of-flight	1026:1061	arg1	MALDI-TOF					1082:1090	MALDI-TOF	1082:1090	MALDI-TOF	1082:1090	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	8	37	theme	desorption/ionization-time-of-flight	1026:1061	arg1	spectrometry					1068:1079	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry	1004:1079	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts	1004:1186	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	8	38	theme	spectrometry	1068:1079	arg1	analysis					1096:1103	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis	1004:1103	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts	1004:1186	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	6	39	theme	different	802:810	arg1	GalAs					812:816	up to two different GalAs	792:816	up to two different GalAs	792:816	Our data suggest that up to two different GalAs are esterified in the RG-II backbone.					
32468908	1	40	theme	ester	232:236	arg1	dimer					251:255	a borate ester cross-linked dimer	223:255	a borate ester cross-linked dimer	223:255	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	8	41	theme	methyl	1202:1207	arg1	etherification					1209:1222	methyl etherification	1202:1222	methyl etherification of the β-linked GalA	1202:1243	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	8	42	theme	MS	1093:1094	arg1	analysis					1096:1103	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis	1004:1103	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts	1004:1186	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	12	43	theme	primary	1780:1786	arg1	sequence					1788:1795	the primary sequence	1776:1795	the primary sequence	1776:1795	Taken together, our data further refine the primary sequence and sequence variation of RG-II and provide additional insight into dimer stability and factors controlling dimer self-assembly.					
32468908	10	44	from	analysis	1381:1388	arg1	RG-II					1423:1427	RG-II	1423:1427	RG-II from different dicots and nonpoalean monocots	1423:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	5	45	theme	esterification	729:742	arg1	status					744:749	the methyl esterification status	718:749	the methyl esterification status of backbone GalAs	718:767	sodium borodeuteride (NaBD4)-reduced RG-II, to identify the methyl esterification status of backbone GalAs.					
32468908	10	46	theme	Ara-[MeFuc	1563:1572	arg1	-Gal-AceA-Rha-Api-					1574:1591	an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-)	1531:1592	an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-)	1531:1592	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	3	47	theme	Methyl	521:526	arg1	esterification					528:541	Methyl esterification	521:541	Methyl esterification of the galacturonan backbone	521:570	Methyl esterification of the galacturonan backbone has also been reported but not confirmed.					
32468908	8	48	theme	A	1118:1118	arg1	analysis					1096:1103	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis	1004:1103	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts	1004:1186	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	10	49	theme	hexasaccharide	1547:1560	arg1	-Gal-AceA-Rha-Api-					1574:1591	an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-)	1531:1592	an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-)	1531:1592	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	11	50	theme	dimer	1665:1669	arg1	formation					1671:1679	dimer formation	1665:1679	dimer formation	1665:1679	To complement these studies, we provide further evidence showing that dimer formation and stability in vitro is cation and borate dependent.					
32468908	1	51	theme	complex	178:184	arg1	polysaccharide					193:206	a structurally complex pectic polysaccharide	163:206	a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants	163:296	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	1	51	theme	complex	178:184	arg1	Rhamnogalacturonan					130:147	Rhamnogalacturonan II	130:150	Rhamnogalacturonan II (RG-II)	130:158	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	6	52	theme	RG-II	840:844	arg1	backbone					846:853	the RG-II backbone	836:853	the RG-II backbone	836:853	Our data suggest that up to two different GalAs are esterified in the RG-II backbone.					
32468908	4	53	theme	new	633:635	arg1	procedure					637:645	a new procedure	631:645	a new procedure	631:645	Here we describe a new procedure, utilizing aq.					
32468908	3	54	theme	galacturonan	550:561	arg1	backbone					563:570	the galacturonan backbone	546:570	the galacturonan backbone	546:570	Methyl esterification of the galacturonan backbone has also been reported but not confirmed.					
32468908	10	55	from	glycoforms	1409:1418	arg1	RG-II					1423:1427	RG-II	1423:1427	RG-II from different dicots and nonpoalean monocots	1423:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	10	55	from	glycoforms	1409:1418	arg1	dicots					1444:1449	different dicots	1434:1449	different dicots	1434:1449	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	10	55	from	glycoforms	1409:1418	arg1	monocots					1466:1473	nonpoalean monocots	1455:1473	nonpoalean monocots	1455:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	10	56	theme	-Gal-AceA-Rha-Api-	1574:1591	arg1	structure					1518:1526	a minimum structure	1508:1526	a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-)	1508:1592	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	1	57	link	cross-linked	238:249	arg1	dimer					251:255	a borate ester cross-linked dimer	223:255	a borate ester cross-linked dimer	223:255	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	2	58	theme	glycosyl	303:310	arg1	sequence					312:319	The glycosyl sequence	299:319	The glycosyl sequence of RG-II	299:328	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	10	59	from	RG-II	1423:1427	arg1	analysis					1381:1388	Our analysis	1377:1388	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots	1377:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	8	60	theme	β-linked	1231:1238	arg1	GalA					1240:1243	the β-linked GalA	1227:1243	the β-linked GalA	1227:1243	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	10	61	from	monocots	1466:1473	arg1	RG-II					1423:1427	RG-II	1423:1427	RG-II from different dicots and nonpoalean monocots	1423:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	10	61	from	monocots	1466:1473	arg1	glycoforms					1409:1418	the sidechain B glycoforms	1393:1418	the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots	1393:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	0	62	theme	Cell	75:78	arg1	Rhamnogalacturonan					107:124	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	Locating Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II.					
32468908	7	63	theme	4-	942:943	arg1	GalA					963:966	3-, 4-, and 3,4-O-methyl GalA	938:966	3-, 4-, and 3,4-O-methyl GalA	938:966	We also adapted a procedure based on methanolysis and NaBD4 reduction to identify 3-, 4-, and 3,4-O-methyl GalA in RG-II.					
32468908	9	64	theme	plant	1362:1366	arg1	species					1368:1374	plant species	1362:1374	plant species	1362:1374	Nevertheless, the extent of these modifications varies between plant species.					
32468908	0	65	theme	Pectic	85:90	arg1	Rhamnogalacturonan					107:124	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	Locating Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II.					
32468908	7	66	theme	3-	938:939	arg1	GalA					963:966	3-, 4-, and 3,4-O-methyl GalA	938:966	3-, 4-, and 3,4-O-methyl GalA	938:966	We also adapted a procedure based on methanolysis and NaBD4 reduction to identify 3-, 4-, and 3,4-O-methyl GalA in RG-II.					
32468908	10	67	contain	has	1504:1506	arg1	sidechain					1494:1502	this sidechain	1489:1502	this sidechain	1489:1502	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	10	67	contain	has	1504:1506	arg2	structure					1518:1526	a minimum structure	1508:1526	a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-)	1508:1592	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	12	68	theme	additional	1841:1850	arg1	insight					1852:1858	additional insight	1841:1858	additional insight into dimer stability and factors controlling dimer self-assembly	1841:1923	Taken together, our data further refine the primary sequence and sequence variation of RG-II and provide additional insight into dimer stability and factors controlling dimer self-assembly.					
32468908	10	69	theme	B	1407:1407	arg1	glycoforms					1409:1418	the sidechain B glycoforms	1393:1418	the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots	1393:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	7	70	theme	NaBD4	910:914	arg1	reduction					916:924	NaBD4 reduction	910:924	NaBD4 reduction	910:924	We also adapted a procedure based on methanolysis and NaBD4 reduction to identify 3-, 4-, and 3,4-O-methyl GalA in RG-II.					
32468908	2	71	theme	A	487:487	arg1	sidechain					489:497	the A sidechain	483:497	the A sidechain	483:497	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	0	72	from	Acids	56:60	arg1	Rhamnogalacturonan					107:124	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	65:127	Locating Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II.					
32468908	2	73	theme	galacturonic	379:390	arg1	etherification					411:424	galacturonic acid (GalA) methyl etherification	379:424	galacturonic acid (GalA) methyl etherification	379:424	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	0	74	theme	Methyl-Etherified	9:25	arg1	Acids					56:60	Methyl-Etherified and Methyl-Esterified Uronic Acids	9:60	Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	9:127	Locating Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II.					
32468908	2	75	theme	glucuronic	430:439	arg1	GlcA					447:450	GlcA	447:450	GlcA	447:450	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	2	75	theme	glucuronic	430:439	arg1	acid					441:444	glucuronic acid	430:444	glucuronic acid (GlcA)	430:451	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	0	76	theme	Methyl-Esterified	31:47	arg1	Acids					56:60	Methyl-Etherified and Methyl-Esterified Uronic Acids	9:60	Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II	9:127	Locating Methyl-Etherified and Methyl-Esterified Uronic Acids in the Plant Cell Wall Pectic Polysaccharide Rhamnogalacturonan II.					
32468908	1	77	theme	vascular	282:289	arg1	plants					291:296	all vascular plants	278:296	all vascular plants	278:296	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	8	78	theme	laser	1020:1024	arg1	MALDI-TOF					1082:1090	MALDI-TOF	1082:1090	MALDI-TOF	1082:1090	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	8	78	theme	laser	1020:1024	arg1	spectrometry					1068:1079	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry	1004:1079	matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts	1004:1186	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32468908	12	79	theme	dimer	1865:1869	arg1	stability					1871:1879	dimer stability	1865:1879	dimer stability	1865:1879	Taken together, our data further refine the primary sequence and sequence variation of RG-II and provide additional insight into dimer stability and factors controlling dimer self-assembly.					
32468908	10	80	theme	nonpoalean	1455:1464	arg1	monocots					1466:1473	nonpoalean monocots	1455:1473	nonpoalean monocots	1455:1473	Our analysis of the sidechain B glycoforms in RG-II from different dicots and nonpoalean monocots suggests that this sidechain has a minimum structure of an O-acetylated hexasaccharide (Ara-[MeFuc]-Gal-AceA-Rha-Api-).					
32468908	5	81	theme	GalAs	763:767	arg1	status					744:749	the methyl esterification status	718:749	the methyl esterification status of backbone GalAs	718:767	sodium borodeuteride (NaBD4)-reduced RG-II, to identify the methyl esterification status of backbone GalAs.					
32468908	9	82	theme	modifications	1333:1345	arg1	extent					1317:1322	the extent	1313:1322	the extent of these modifications	1313:1345	Nevertheless, the extent of these modifications varies between plant species.					
32468908	12	83	theme	RG-II	1823:1827	arg1	sequence					1788:1795	the primary sequence	1776:1795	the primary sequence	1776:1795	Taken together, our data further refine the primary sequence and sequence variation of RG-II and provide additional insight into dimer stability and factors controlling dimer self-assembly.					
32468908	12	83	theme	RG-II	1823:1827	arg1	variation					1810:1818	sequence variation	1801:1818	sequence variation	1801:1818	Taken together, our data further refine the primary sequence and sequence variation of RG-II and provide additional insight into dimer stability and factors controlling dimer self-assembly.					
32468908	12	84	theme	sequence	1801:1808	arg1	variation					1810:1818	sequence variation	1801:1818	sequence variation	1801:1818	Taken together, our data further refine the primary sequence and sequence variation of RG-II and provide additional insight into dimer stability and factors controlling dimer self-assembly.					
32468908	2	85	theme	RG-II	324:328	arg1	sequence					312:319	The glycosyl sequence	299:319	The glycosyl sequence of RG-II	299:328	The glycosyl sequence of RG-II is largely conserved, but there is evidence that galacturonic acid (GalA) methyl etherification and glucuronic acid (GlcA) methyl esterification vary in the A sidechain across plant species.					
32468908	1	86	theme	cross-linked	238:249	arg1	dimer					251:255	a borate ester cross-linked dimer	223:255	a borate ester cross-linked dimer	223:255	Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that exists as a borate ester cross-linked dimer in the cell walls of all vascular plants.					
32468908	8	87	link	β-linked	1231:1238	arg1	GalA					1240:1243	the β-linked GalA	1227:1243	the β-linked GalA	1227:1243	These data, together with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF) MS analysis of sidechain A generated from selected RG-IIs and their NaBD4-reduced counterparts, suggest that methyl etherification of the β-linked GalA and methyl esterification of the GlcA are widespread.					
32179120	0	0	theme	CD4+	90:93	arg1	cells					97:101	CD4+ T cells	90:101	CD4+ T cells	90:101	Extraction of polysaccharides from maca: Characterization and immunoregulatory effects on CD4+ T cells.					
32179120	6	1	theme	CD4+	876:879	arg1	cells					883:887	CD4+ T cells	876:887	CD4+ T cells	876:887	However, the effects of MCP2 were stronger compared to the other three components, not only in promoting the proliferation of CD4+ T cells but also in terms of secretion of interferon-γ (IFN-γ).					
32179120	8	2	theme	MCPs	1148:1151	arg1	galactosamine					1124:1136	the galactosamine	1120:1136	the galactosamine (GalN) of MCPs	1120:1151	The results suggested that the molecular weight and the galactosamine (GalN) of MCPs might be determining factors for its bioactivity.					
32179120	8	2	theme	MCPs	1148:1151	arg1	factors					1174:1180	determining factors	1162:1180	determining factors for its bioactivity	1162:1200	The results suggested that the molecular weight and the galactosamine (GalN) of MCPs might be determining factors for its bioactivity.					
32179120	8	2	theme	MCPs	1148:1151	arg1	GalN					1139:1142	GalN	1139:1142	GalN	1139:1142	The results suggested that the molecular weight and the galactosamine (GalN) of MCPs might be determining factors for its bioactivity.					
32179120	8	2	theme	MCPs	1148:1151	arg1	weight					1109:1114	the molecular weight	1095:1114	the molecular weight	1095:1114	The results suggested that the molecular weight and the galactosamine (GalN) of MCPs might be determining factors for its bioactivity.					
32179120	3	3	theme	water-soluble	292:304	arg1	MCPs					306:309	Four water-soluble MCPs	287:309	Four water-soluble MCPs	287:309	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	9	4	contain	have	1262:1265	arg1	MCP2					1235:1238	the MCP2	1231:1238	the MCP2 isolated in our study	1231:1260	These findings suggest that the MCP2 isolated in our study have immune potentiation effects on CD4+ T cells.					
32179120	9	4	contain	have	1262:1265	arg2	effects					1287:1293	immune potentiation effects	1267:1293	immune potentiation effects	1267:1293	These findings suggest that the MCP2 isolated in our study have immune potentiation effects on CD4+ T cells.					
32179120	6	5	theme	interferon-γ	923:934	arg1	secretion					910:918	secretion	910:918	secretion of interferon-γ (IFN-γ)	910:942	However, the effects of MCP2 were stronger compared to the other three components, not only in promoting the proliferation of CD4+ T cells but also in terms of secretion of interferon-γ (IFN-γ).					
32179120	3	6	theme	graded	537:542	arg1	precipitation					552:564	graded ethanol precipitation	537:564	graded ethanol precipitation	537:564	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	5	7	theme	T	741:741	arg1	cells					743:747	CD4+ T cells	736:747	CD4+ T cells	736:747	The experimental results indicated that all MCPs had immunoregulatory effects on CD4+ T cells.					
32179120	0	8	theme	T	95:95	arg1	cells					97:101	CD4+ T cells	90:101	CD4+ T cells	90:101	Extraction of polysaccharides from maca: Characterization and immunoregulatory effects on CD4+ T cells.					
32179120	4	9	theme	T	612:612	arg1	cells					614:618	CD4+ T cells	607:618	CD4+ T cells	607:618	The immunoregulatory effects of MCPs on CD4+ T cells were evaluated for the first time.					
32179120	4	10	theme	immunoregulatory	571:586	arg1	effects					588:594	The immunoregulatory effects	567:594	The immunoregulatory effects of MCPs on CD4+ T cells	567:618	The immunoregulatory effects of MCPs on CD4+ T cells were evaluated for the first time.					
32179120	7	11	theme	MCPs	1001:1004	arg1	weight					959:964	molecular weight	949:964	molecular weight	949:964	The molecular weight and monosaccharide compositions of MCPs were analyzed to explore the structure-activity relationship.					
32179120	7	11	theme	MCPs	1001:1004	arg1	compositions					985:996	monosaccharide compositions	970:996	monosaccharide compositions	970:996	The molecular weight and monosaccharide compositions of MCPs were analyzed to explore the structure-activity relationship.					
32179120	0	12	from	Characterization	41:56	arg1	cells					97:101	CD4+ T cells	90:101	CD4+ T cells	90:101	Extraction of polysaccharides from maca: Characterization and immunoregulatory effects on CD4+ T cells.					
32179120	7	13	theme	monosaccharide	970:983	arg1	compositions					985:996	monosaccharide compositions	970:996	monosaccharide compositions	970:996	The molecular weight and monosaccharide compositions of MCPs were analyzed to explore the structure-activity relationship.					
32179120	5	14	contain	had	704:706	arg1	MCPs					699:702	all MCPs	695:702	all MCPs	695:702	The experimental results indicated that all MCPs had immunoregulatory effects on CD4+ T cells.					
32179120	5	14	contain	had	704:706	arg2	effects					725:731	immunoregulatory effects	708:731	immunoregulatory effects	708:731	The experimental results indicated that all MCPs had immunoregulatory effects on CD4+ T cells.					
32179120	2	15	theme	T	256:256	arg1	cells					258:262	CD4+ T cells	251:262	CD4+ T cells	251:262	However, the effects of MCPs on CD4+ T cells have not been studied.					
32179120	3	16	dep	MCP4	470:473	arg1	Mws					476:478	Mws	476:478	Mws of 15.7, 12.6, and 12.1 kDa	476:506	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	9	17	theme	potentiation	1274:1285	arg1	effects					1287:1293	immune potentiation effects	1267:1293	immune potentiation effects	1267:1293	These findings suggest that the MCP2 isolated in our study have immune potentiation effects on CD4+ T cells.					
32179120	8	18	theme	determining	1162:1172	arg1	galactosamine					1124:1136	the galactosamine	1120:1136	the galactosamine (GalN) of MCPs	1120:1151	The results suggested that the molecular weight and the galactosamine (GalN) of MCPs might be determining factors for its bioactivity.					
32179120	8	18	theme	determining	1162:1172	arg1	factors					1174:1180	determining factors	1162:1180	determining factors for its bioactivity	1162:1200	The results suggested that the molecular weight and the galactosamine (GalN) of MCPs might be determining factors for its bioactivity.					
32179120	8	18	theme	determining	1162:1172	arg1	weight					1109:1114	the molecular weight	1095:1114	the molecular weight	1095:1114	The results suggested that the molecular weight and the galactosamine (GalN) of MCPs might be determining factors for its bioactivity.					
32179120	2	19	theme	CD4+	251:254	arg1	cells					258:262	CD4+ T cells	251:262	CD4+ T cells	251:262	However, the effects of MCPs on CD4+ T cells have not been studied.					
32179120	5	20	theme	immunoregulatory	708:723	arg1	effects					725:731	immunoregulatory effects	708:731	immunoregulatory effects	708:731	The experimental results indicated that all MCPs had immunoregulatory effects on CD4+ T cells.					
32179120	0	21	from	maca	35:38	arg1	Extraction					0:9	Extraction	0:9	Extraction of polysaccharides from maca	0:38	Extraction of polysaccharides from maca: Characterization and immunoregulatory effects on CD4+ T cells.					
32179120	3	22	dep	MCP3	425:428	arg1	Mws					431:433	Mws	431:433	Mws of 110.6, 58.1, and 38.9 kDa	431:462	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	4	23	theme	MCPs	599:602	arg1	effects					588:594	The immunoregulatory effects	567:594	The immunoregulatory effects of MCPs on CD4+ T cells	567:618	The immunoregulatory effects of MCPs on CD4+ T cells were evaluated for the first time.					
32179120	6	24	theme	other	809:813	arg1	components					821:830	the other three components	805:830	the other three components	805:830	However, the effects of MCP2 were stronger compared to the other three components, not only in promoting the proliferation of CD4+ T cells but also in terms of secretion of interferon-γ (IFN-γ).					
32179120	7	25	dep	weight	959:964	arg1	The					945:947	The	945:947	The	945:947	The molecular weight and monosaccharide compositions of MCPs were analyzed to explore the structure-activity relationship.					
32179120	6	26	theme	cells	883:887	arg1	proliferation					859:871	the proliferation	855:871	the proliferation of CD4+ T cells	855:887	However, the effects of MCP2 were stronger compared to the other three components, not only in promoting the proliferation of CD4+ T cells but also in terms of secretion of interferon-γ (IFN-γ).					
32179120	3	27	theme	molecular	341:349	arg1	[Mws					359:362	weight-average molecular weights [Mws]	326:363	weight-average molecular weights [Mws] of 896.1 and 276.6 kDa	326:386	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	9	28	theme	CD4+	1298:1301	arg1	cells					1305:1309	CD4+ T cells	1298:1309	CD4+ T cells	1298:1309	These findings suggest that the MCP2 isolated in our study have immune potentiation effects on CD4+ T cells.					
32179120	1	29	from	effects	125:131	arg1	macrophages					167:177	macrophages	167:177	macrophages	167:177	The immunomodulatory effects of maca polysaccharides (MCPs) on macrophages have been demonstrated in many studies.					
32179120	3	30	theme	38.9 kDa	455:462	arg1	Mws					431:433	Mws	431:433	Mws of 110.6, 58.1, and 38.9 kDa	431:462	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	2	31	from	effects	232:238	arg1	cells					258:262	CD4+ T cells	251:262	CD4+ T cells	251:262	However, the effects of MCPs on CD4+ T cells have not been studied.					
32179120	3	32	theme	337.8	403:407	arg1	Mws					396:398	Mws	396:398	Mws of 337.8 and 219.0 kDa	396:421	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	3	33	theme	weights	351:357	arg1	[Mws					359:362	weight-average molecular weights [Mws]	326:363	weight-average molecular weights [Mws] of 896.1 and 276.6 kDa	326:386	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	5	34	theme	CD4+	736:739	arg1	cells					743:747	CD4+ T cells	736:747	CD4+ T cells	736:747	The experimental results indicated that all MCPs had immunoregulatory effects on CD4+ T cells.					
32179120	7	35	theme	molecular	949:957	arg1	weight					959:964	molecular weight	949:964	molecular weight	949:964	The molecular weight and monosaccharide compositions of MCPs were analyzed to explore the structure-activity relationship.					
32179120	9	36	theme	immune	1267:1272	arg1	effects					1287:1293	immune potentiation effects	1267:1293	immune potentiation effects	1267:1293	These findings suggest that the MCP2 isolated in our study have immune potentiation effects on CD4+ T cells.					
32179120	1	37	theme	many	205:208	arg1	studies					210:216	many studies	205:216	many studies	205:216	The immunomodulatory effects of maca polysaccharides (MCPs) on macrophages have been demonstrated in many studies.					
32179120	3	38	theme	276.6 kDa	378:386	arg1	[Mws					359:362	weight-average molecular weights [Mws]	326:363	weight-average molecular weights [Mws] of 896.1 and 276.6 kDa	326:386	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	0	39	from	effects	79:85	arg1	cells					97:101	CD4+ T cells	90:101	CD4+ T cells	90:101	Extraction of polysaccharides from maca: Characterization and immunoregulatory effects on CD4+ T cells.					
32179120	0	40	dep	Characterization	41:56	arg1	Extraction					0:9	Extraction	0:9	Extraction of polysaccharides from maca	0:38	Extraction of polysaccharides from maca: Characterization and immunoregulatory effects on CD4+ T cells.					
32179120	5	41	theme	experimental	659:670	arg1	results					672:678	The experimental results	655:678	The experimental results	655:678	The experimental results indicated that all MCPs had immunoregulatory effects on CD4+ T cells.					
32179120	3	42	theme	12.1 kDa	499:506	arg1	Mws					476:478	Mws	476:478	Mws of 15.7, 12.6, and 12.1 kDa	476:506	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	3	43	dep	MCP2	390:393	arg1	Mws					396:398	Mws	396:398	Mws of 337.8 and 219.0 kDa	396:421	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	1	44	theme	immunomodulatory	108:123	arg1	effects					125:131	The immunomodulatory effects	104:131	The immunomodulatory effects of maca polysaccharides (MCPs) on macrophages	104:177	The immunomodulatory effects of maca polysaccharides (MCPs) on macrophages have been demonstrated in many studies.					
32179120	3	45	theme	896.1	368:372	arg1	[Mws					359:362	weight-average molecular weights [Mws]	326:363	weight-average molecular weights [Mws] of 896.1 and 276.6 kDa	326:386	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	6	46	theme	MCP2	774:777	arg1	stronger					784:791	stronger	784:791	stronger	784:791	However, the effects of MCP2 were stronger compared to the other three components, not only in promoting the proliferation of CD4+ T cells but also in terms of secretion of interferon-γ (IFN-γ).					
32179120	6	46	theme	MCP2	774:777	arg1	effects					763:769	the effects	759:769	the effects of MCP2	759:777	However, the effects of MCP2 were stronger compared to the other three components, not only in promoting the proliferation of CD4+ T cells but also in terms of secretion of interferon-γ (IFN-γ).					
32179120	3	47	dep	MCP1	320:323	arg1	[Mws					359:362	weight-average molecular weights [Mws]	326:363	weight-average molecular weights [Mws] of 896.1 and 276.6 kDa	326:386	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	9	48	theme	T	1303:1303	arg1	cells					1305:1309	CD4+ T cells	1298:1309	CD4+ T cells	1298:1309	These findings suggest that the MCP2 isolated in our study have immune potentiation effects on CD4+ T cells.					
32179120	6	49	dep	stronger	784:791	arg1	only					837:840	only	837:840	only	837:840	However, the effects of MCP2 were stronger compared to the other three components, not only in promoting the proliferation of CD4+ T cells but also in terms of secretion of interferon-γ (IFN-γ).					
32179120	4	50	from	effects	588:594	arg1	cells					614:618	CD4+ T cells	607:618	CD4+ T cells	607:618	The immunoregulatory effects of MCPs on CD4+ T cells were evaluated for the first time.					
32179120	4	51	theme	first	643:647	arg1	time					649:652	the first time	639:652	the first time	639:652	The immunoregulatory effects of MCPs on CD4+ T cells were evaluated for the first time.					
32179120	3	52	theme	12.6	489:492	arg1	Mws					476:478	Mws	476:478	Mws of 15.7, 12.6, and 12.1 kDa	476:506	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	3	53	theme	219.0 kDa	413:421	arg1	Mws					396:398	Mws	396:398	Mws of 337.8 and 219.0 kDa	396:421	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	4	54	theme	CD4+	607:610	arg1	cells					614:618	CD4+ T cells	607:618	CD4+ T cells	607:618	The immunoregulatory effects of MCPs on CD4+ T cells were evaluated for the first time.					
32179120	8	55	theme	molecular	1099:1107	arg1	factors					1174:1180	determining factors	1162:1180	determining factors for its bioactivity	1162:1200	The results suggested that the molecular weight and the galactosamine (GalN) of MCPs might be determining factors for its bioactivity.					
32179120	8	55	theme	molecular	1099:1107	arg1	weight					1109:1114	the molecular weight	1095:1114	the molecular weight	1095:1114	The results suggested that the molecular weight and the galactosamine (GalN) of MCPs might be determining factors for its bioactivity.					
32179120	3	56	theme	58.1	445:448	arg1	Mws					431:433	Mws	431:433	Mws of 110.6, 58.1, and 38.9 kDa	431:462	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	2	57	theme	MCPs	243:246	arg1	effects					232:238	the effects	228:238	the effects of MCPs on CD4+ T cells	228:262	However, the effects of MCPs on CD4+ T cells have not been studied.					
32179120	1	58	theme	maca	136:139	arg1	MCPs					158:161	MCPs	158:161	MCPs	158:161	The immunomodulatory effects of maca polysaccharides (MCPs) on macrophages have been demonstrated in many studies.					
32179120	1	58	theme	maca	136:139	arg1	polysaccharides					141:155	maca polysaccharides	136:155	maca polysaccharides (MCPs)	136:162	The immunomodulatory effects of maca polysaccharides (MCPs) on macrophages have been demonstrated in many studies.					
32179120	7	59	theme	structure-activity	1035:1052	arg1	relationship					1054:1065	the structure-activity relationship	1031:1065	the structure-activity relationship	1031:1065	The molecular weight and monosaccharide compositions of MCPs were analyzed to explore the structure-activity relationship.					
32179120	6	60	theme	secretion	910:918	arg1	terms					901:905	terms	901:905	terms of secretion of interferon-γ (IFN-γ)	901:942	However, the effects of MCP2 were stronger compared to the other three components, not only in promoting the proliferation of CD4+ T cells but also in terms of secretion of interferon-γ (IFN-γ).					
32179120	3	61	theme	15.7	483:486	arg1	Mws					476:478	Mws	476:478	Mws of 15.7, 12.6, and 12.1 kDa	476:506	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	6	62	theme	T	881:881	arg1	cells					883:887	CD4+ T cells	876:887	CD4+ T cells	876:887	However, the effects of MCP2 were stronger compared to the other three components, not only in promoting the proliferation of CD4+ T cells but also in terms of secretion of interferon-γ (IFN-γ).					
32179120	1	63	theme	polysaccharides	141:155	arg1	effects					125:131	The immunomodulatory effects	104:131	The immunomodulatory effects of maca polysaccharides (MCPs) on macrophages	104:177	The immunomodulatory effects of maca polysaccharides (MCPs) on macrophages have been demonstrated in many studies.					
32179120	0	64	theme	immunoregulatory	62:77	arg1	effects					79:85	immunoregulatory effects	62:85	immunoregulatory effects on CD4+ T cells	62:101	Extraction of polysaccharides from maca: Characterization and immunoregulatory effects on CD4+ T cells.					
32179120	3	65	theme	ethanol	544:550	arg1	precipitation					552:564	graded ethanol precipitation	537:564	graded ethanol precipitation	537:564	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	0	66	theme	polysaccharides	14:28	arg1	Extraction					0:9	Extraction	0:9	Extraction of polysaccharides from maca	0:38	Extraction of polysaccharides from maca: Characterization and immunoregulatory effects on CD4+ T cells.					
32179120	3	67	theme	110.6	438:442	arg1	Mws					431:433	Mws	431:433	Mws of 110.6, 58.1, and 38.9 kDa	431:462	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
32179120	3	68	theme	weight-average	326:339	arg1	[Mws					359:362	weight-average molecular weights [Mws]	326:363	weight-average molecular weights [Mws] of 896.1 and 276.6 kDa	326:386	Four water-soluble MCPs, labeled MCP1 (weight-average molecular weights [Mws] of 896.1 and 276.6 kDa), MCP2 (Mws of 337.8 and 219.0 kDa), MCP3 (Mws of 110.6, 58.1, and 38.9 kDa), and MCP4 (Mws of 15.7, 12.6, and 12.1 kDa), were obtained from maca by graded ethanol precipitation.					
33167499	1	0	theme	wall	323:326	arg1	role					276:279	the central role	264:279	the central role	264:279	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	1	0	theme	wall	323:326	arg1	component					297:305	a structural component	284:305	a structural component of a yeast cell wall (CW)	284:331	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	4	1	contain	contain	706:712	arg2	molecules					719:727	Bgl2 molecules	714:727	Bgl2 molecules with unmodified peptides	714:752	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	4	1	contain	contain	706:712	arg1	pools					700:704	Identified pools	689:704	Identified pools	689:704	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	4	2	theme	Identified	689:698	arg1	pools					700:704	Identified pools	689:704	Identified pools	689:704	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	9	3	theme	distribution	1518:1529	arg1	scheme					1508:1513	The hypothetical scheme	1491:1513	The hypothetical scheme of distribution of Bgl2 inside CW	1491:1547	The hypothetical scheme of distribution of Bgl2 inside CW is represented.					
33167499	4	4	theme	CW	879:880	arg1	proteins					882:889	other CW proteins	873:889	other CW proteins	873:889	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	7	5	from	CW	1214:1215	arg1	isolation					1199:1207	isolation	1199:1207	isolation from CW	1199:1215	Bgl2 from the pool of unmodified and monophosphorylated molecules demonstrates the ability to fibrillate after isolation from CW.					
33167499	6	6	theme	attachment	1053:1062	arg1	mode					1041:1044	the mode	1037:1044	the mode of its attachment and enzymatic activity	1037:1085	Glutathione affects Bgl2 conformation, probably resulting in the mode of its attachment and enzymatic activity.					
33167499	0	7	theme	Glucanosyltransglycosylases	99:125	arg1	Attachment					64:73	Attachment	64:73	Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall	64:144	The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning.					
33167499	3	8	theme	attachment	677:686	arg1	firmness					665:672	various firmness	657:672	various firmness of attachment	657:686	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	8	9	theme	reversible	1396:1405	arg1	modifications					1426:1438	reversible post-translational modifications	1396:1438	reversible post-translational modifications	1396:1438	Revealing of Bgl2 microcompartments and their mosaic arrangement summarized with the results obtained give the evidence that the functioning of ncGTGs in CW can be controlled by reversible post-translational modifications and facilitated due to their compact localization.					
33167499	0	10	theme	Bound	93:97	arg1	Glucanosyltransglycosylases					99:125	Non-Covalently Bound Glucanosyltransglycosylases	78:125	Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall	78:144	The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning.					
33167499	7	11	theme	unmodified	1110:1119	arg1	molecules					1144:1152	unmodified and monophosphorylated molecules	1110:1152	unmodified and monophosphorylated molecules	1110:1152	Bgl2 from the pool of unmodified and monophosphorylated molecules demonstrates the ability to fibrillate after isolation from CW.					
33167499	1	12	theme	central	268:274	arg1	role					276:279	the central role	264:279	the central role	264:279	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	1	12	theme	central	268:274	arg1	component					297:305	a structural component	284:305	a structural component of a yeast cell wall (CW)	284:331	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	0	13	theme	Yeast	130:134	arg1	Wall					141:144	Yeast Cell Wall	130:144	Yeast Cell Wall	130:144	The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning.					
33167499	2	14	theme	bound	378:382	arg1	ncGTGs					413:418	ncGTGs	413:418	ncGTGs	413:418	Regulation of functioning of non-covalently bound glucanosyltransglycosylases (ncGTGs) that have to remodel mGC to provide CW extension is poorly understood.					
33167499	2	14	theme	bound	378:382	arg1	glucanosyltransglycosylases					384:410	non-covalently bound glucanosyltransglycosylases	363:410	non-covalently bound glucanosyltransglycosylases (ncGTGs) that have to remodel mGC to provide CW extension	363:468	Regulation of functioning of non-covalently bound glucanosyltransglycosylases (ncGTGs) that have to remodel mGC to provide CW extension is poorly understood.					
33167499	4	15	theme	ones	826:829	arg1	presence					789:796	presence	789:796	presence	789:796	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	3	16	theme	main	516:519	arg1	ncGTGs					521:526	the main ncGTGs	512:526	the main ncGTGs Bgl2 and Scw4	512:540	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	16	theme	main	516:519	arg1	pools					631:635	different pools	621:635	different pools of molecules having various firmness of attachment	621:686	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	16	theme	main	516:519	arg1	Bgl2					528:531	Bgl2	528:531	Bgl2	528:531	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	16	theme	main	516:519	arg1	Scw4					537:540	Scw4	537:540	Scw4	537:540	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	0	17	theme	Post-Translational	4:21	arg1	Modifications					23:35	The Post-Translational Modifications	0:35	The Post-Translational Modifications	0:35	The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning.					
33167499	3	18	contain	have	542:545	arg1	ncGTGs					521:526	the main ncGTGs	512:526	the main ncGTGs Bgl2 and Scw4	512:540	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	18	contain	have	542:545	arg1	pools					631:635	different pools	621:635	different pools of molecules having various firmness of attachment	621:686	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	18	contain	have	542:545	arg1	Bgl2					528:531	Bgl2	528:531	Bgl2	528:531	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	18	contain	have	542:545	arg1	Scw4					537:540	Scw4	537:540	Scw4	537:540	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	18	contain	have	542:545	arg2	residues					583:590	phosphorylated and glutathionylated residues	547:590	phosphorylated and glutathionylated residues	547:590	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	4	19	theme	unmodified	734:743	arg1	peptides					745:752	unmodified peptides	734:752	unmodified peptides	734:752	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	3	20	theme	phosphorylated	547:560	arg1	residues					583:590	phosphorylated and glutathionylated residues	547:590	phosphorylated and glutathionylated residues	547:590	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	2	21	theme	functioning	348:358	arg1	Regulation					334:343	Regulation	334:343	Regulation of functioning of non-covalently bound glucanosyltransglycosylases (ncGTGs) that have to remodel mGC to provide CW extension	334:468	Regulation of functioning of non-covalently bound glucanosyltransglycosylases (ncGTGs) that have to remodel mGC to provide CW extension is poorly understood.					
33167499	7	22	theme	monophosphorylated	1125:1142	arg1	molecules					1144:1152	unmodified and monophosphorylated molecules	1110:1152	unmodified and monophosphorylated molecules	1110:1152	Bgl2 from the pool of unmodified and monophosphorylated molecules demonstrates the ability to fibrillate after isolation from CW.					
33167499	0	23	theme	Wall	141:144	arg1	Glucanosyltransglycosylases					99:125	Non-Covalently Bound Glucanosyltransglycosylases	78:125	Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall	78:144	The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning.					
33167499	5	24	theme	distribution	912:923	arg1	Correlation					892:902	Correlation	892:902	Correlation of Bgl2 distribution among pools and its N-glycosylation	892:959	Correlation of Bgl2 distribution among pools and its N-glycosylation was not found.					
33167499	5	25	theme	Bgl2	907:910	arg1	distribution					912:923	Bgl2 distribution	907:923	Bgl2 distribution	907:923	Correlation of Bgl2 distribution among pools and its N-glycosylation was not found.					
33167499	0	26	theme	Cell	136:139	arg1	Wall					141:144	Yeast Cell Wall	130:144	Yeast Cell Wall	130:144	The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning.					
33167499	9	27	theme	Bgl2	1534:1537	arg1	inside					1539:1544	Bgl2 inside CW	1534:1547	Bgl2 inside CW	1534:1547	The hypothetical scheme of distribution of Bgl2 inside CW is represented.					
33167499	2	28	theme	CW	457:458	arg1	extension					460:468	CW extension	457:468	CW extension	457:468	Regulation of functioning of non-covalently bound glucanosyltransglycosylases (ncGTGs) that have to remodel mGC to provide CW extension is poorly understood.					
33167499	1	29	theme	structural	286:295	arg1	role					276:279	the central role	264:279	the central role	264:279	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	1	29	theme	structural	286:295	arg1	component					297:305	a structural component	284:305	a structural component of a yeast cell wall (CW)	284:331	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	4	30	dep	presence	850:857	arg1	the					846:848	the	846:848	the	846:848	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	8	31	theme	Bgl2	1231:1234	arg1	microcompartments					1236:1252	Bgl2 microcompartments	1231:1252	Bgl2 microcompartments	1231:1252	Revealing of Bgl2 microcompartments and their mosaic arrangement summarized with the results obtained give the evidence that the functioning of ncGTGs in CW can be controlled by reversible post-translational modifications and facilitated due to their compact localization.					
33167499	3	32	contain	having	650:655	arg2	firmness					665:672	various firmness	657:672	various firmness of attachment	657:686	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	32	contain	having	650:655	arg1	molecules					640:648	molecules	640:648	molecules having various firmness of attachment	640:686	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	1	33	attach	linked	198:203	arg1	proteins					208:215	proteins	208:215	proteins	208:215	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	1	33	attach	linked	198:203	arg2	mega-glycoconjugate					230:248	a natural mega-glycoconjugate	220:248	a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW)	220:331	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	1	33	attach	linked	198:203	arg2	Glucan					191:196	Glucan	191:196	Glucan linked to proteins	191:215	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	7	34	from	pool	1102:1105	arg1	Bgl2					1088:1091	Bgl2	1088:1091	Bgl2 from the pool of unmodified and monophosphorylated molecules	1088:1152	Bgl2 from the pool of unmodified and monophosphorylated molecules demonstrates the ability to fibrillate after isolation from CW.					
33167499	8	35	theme	mosaic	1264:1269	arg1	arrangement					1271:1281	their mosaic arrangement	1258:1281	their mosaic arrangement	1258:1281	Revealing of Bgl2 microcompartments and their mosaic arrangement summarized with the results obtained give the evidence that the functioning of ncGTGs in CW can be controlled by reversible post-translational modifications and facilitated due to their compact localization.					
33167499	9	36	theme	inside	1539:1544	arg1	distribution					1518:1529	distribution	1518:1529	distribution of Bgl2 inside CW	1518:1547	The hypothetical scheme of distribution of Bgl2 inside CW is represented.					
33167499	3	37	theme	different	621:629	arg1	pools					631:635	different pools	621:635	different pools of molecules having various firmness of attachment	621:686	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	37	theme	different	621:629	arg1	ncGTGs					521:526	the main ncGTGs	512:526	the main ncGTGs Bgl2 and Scw4	512:540	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	4	38	theme	modified	817:824	arg1	ones					826:829	modified ones	817:829	modified ones	817:829	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	4	39	dep	presence	789:796	arg1	the					785:787	the	785:787	the	785:787	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	4	40	theme	proteins	882:889	arg1	absence					862:868	absence	862:868	absence	862:868	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	4	40	theme	proteins	882:889	arg1	presence					850:857	presence	850:857	presence	850:857	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	3	41	theme	various	657:663	arg1	firmness					665:672	various firmness	657:672	various firmness of attachment	657:686	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	42	theme	glutathionylated	566:581	arg1	residues					583:590	phosphorylated and glutathionylated residues	547:590	phosphorylated and glutathionylated residues	547:590	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	8	43	theme	compact	1469:1475	arg1	localization					1477:1488	their compact localization	1463:1488	their compact localization	1463:1488	Revealing of Bgl2 microcompartments and their mosaic arrangement summarized with the results obtained give the evidence that the functioning of ncGTGs in CW can be controlled by reversible post-translational modifications and facilitated due to their compact localization.					
33167499	1	44	theme	yeast	312:316	arg1	CW					329:330	CW	329:330	CW	329:330	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	1	44	theme	yeast	312:316	arg1	wall					323:326	a yeast cell wall	310:326	a yeast cell wall (CW)	310:331	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	4	45	theme	other	873:877	arg1	proteins					882:889	other CW proteins	873:889	other CW proteins	873:889	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	6	46	theme	Bgl2	996:999	arg1	conformation					1001:1012	Bgl2 conformation	996:1012	Bgl2 conformation	996:1012	Glutathione affects Bgl2 conformation, probably resulting in the mode of its attachment and enzymatic activity.					
33167499	3	47	dep	ncGTGs	521:526	arg1	ncGTGs					521:526	the main ncGTGs	512:526	the main ncGTGs Bgl2 and Scw4	512:540	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	47	dep	ncGTGs	521:526	arg1	Scw4					537:540	Scw4	537:540	Scw4	537:540	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	47	dep	ncGTGs	521:526	arg1	Bgl2					528:531	Bgl2	528:531	Bgl2	528:531	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	1	48	theme	natural	222:228	arg1	Glucan					191:196	Glucan	191:196	Glucan linked to proteins	191:215	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	1	48	theme	natural	222:228	arg1	mGC					251:253	mGC	251:253	mGC	251:253	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	1	48	theme	natural	222:228	arg1	mega-glycoconjugate					230:248	a natural mega-glycoconjugate	220:248	a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW)	220:331	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	3	49	theme	molecules	640:648	arg1	pools					631:635	different pools	621:635	different pools of molecules having various firmness of attachment	621:686	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	3	49	theme	molecules	640:648	arg1	ncGTGs					521:526	the main ncGTGs	512:526	the main ncGTGs Bgl2 and Scw4	512:540	We demonstrate that the main ncGTGs Bgl2 and Scw4 have phosphorylated and glutathionylated residues and are represented in CW as different pools of molecules having various firmness of attachment.					
33167499	8	50	theme	post-translational	1407:1424	arg1	modifications					1426:1438	reversible post-translational modifications	1396:1438	reversible post-translational modifications	1396:1438	Revealing of Bgl2 microcompartments and their mosaic arrangement summarized with the results obtained give the evidence that the functioning of ncGTGs in CW can be controlled by reversible post-translational modifications and facilitated due to their compact localization.					
33167499	4	51	theme	Bgl2	714:717	arg1	molecules					719:727	Bgl2 molecules	714:727	Bgl2 molecules with unmodified peptides	714:752	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	4	52	with	molecules	719:727	arg1	peptides					745:752	unmodified peptides	734:752	unmodified peptides	734:752	Identified pools contain Bgl2 molecules with unmodified peptides, but differ from each other in the presence and combination of modified ones, as well as in the presence or absence of other CW proteins.					
33167499	0	53	theme	Attachment	64:73	arg1	Modifications					23:35	The Post-Translational Modifications	0:35	The Post-Translational Modifications	0:35	The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning.					
33167499	0	53	theme	Attachment	64:73	arg1	Localization					38:49	Localization	38:49	Localization	38:49	The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning.					
33167499	0	53	theme	Attachment	64:73	arg1	Mode					56:59	Mode	56:59	Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding	56:170	The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning.					
33167499	9	54	theme	hypothetical	1495:1506	arg1	scheme					1508:1513	The hypothetical scheme	1491:1513	The hypothetical scheme of distribution of Bgl2 inside CW	1491:1547	The hypothetical scheme of distribution of Bgl2 inside CW is represented.					
33167499	6	55	theme	activity	1078:1085	arg1	mode					1041:1044	the mode	1037:1044	the mode of its attachment and enzymatic activity	1037:1085	Glutathione affects Bgl2 conformation, probably resulting in the mode of its attachment and enzymatic activity.					
33167499	1	56	theme	cell	318:321	arg1	CW					329:330	CW	329:330	CW	329:330	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	1	56	theme	cell	318:321	arg1	wall					323:326	a yeast cell wall	310:326	a yeast cell wall (CW)	310:331	Glucan linked to proteins is a natural mega-glycoconjugate (mGC) playing the central role as a structural component of a yeast cell wall (CW).					
33167499	7	57	theme	molecules	1144:1152	arg1	pool					1102:1105	the pool	1098:1105	the pool of unmodified and monophosphorylated molecules	1098:1152	Bgl2 from the pool of unmodified and monophosphorylated molecules demonstrates the ability to fibrillate after isolation from CW.					
33167499	6	58	theme	enzymatic	1068:1076	arg1	activity					1078:1085	enzymatic activity	1068:1085	enzymatic activity	1068:1085	Glutathione affects Bgl2 conformation, probably resulting in the mode of its attachment and enzymatic activity.					
32896318	3	0	attach	present	680:686	arg2	networks					671:678	the polysaccharide networks	652:678	the polysaccharide networks present in the cell wall	652:703	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	3	0	attach	present	680:686	arg1	wall					700:703	the cell wall	691:703	the cell wall	691:703	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	9	1	used	used	1603:1606	arg2	enrichment					1553:1562	enrichment	1553:1562	enrichment	1553:1562	For the latter, we provide an AGP-specific protocol to ensure enrichment of members of this group, which can be used independently or in conjunction with the described protocol.					
32896318	4	2	theme	cell	818:821	arg1	glycoprotein					831:842	a presumed cell surface glycoprotein	807:842	a presumed cell surface glycoprotein	807:842	In this chapter, we guide the reader through a protocol aimed to address the glycosylation status of a presumed cell surface glycoprotein.					
32896318	3	3	theme	additional	512:521	arg1	layers					523:528	additional layers	512:528	additional layers of complexity	512:542	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	2	4	from	localization	317:328	arg1	backbone					424:431	the protein backbone	412:431	the protein backbone	412:431	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	5	5	gly	glycoprotein	961:972	arg1	glycoprotein					961:972	recombinant glycoprotein production	949:983	recombinant glycoprotein production	949:983	First, we discuss the advantages and disadvantages of using plants as homologous expression systems for recombinant glycoprotein production.					
32896318	3	6	from	present	680:686	arg1	wall					700:703	the cell wall	691:703	the cell wall	691:703	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	3	7	gly	glycoproteins	566:578	arg1	glycoproteins					566:578	cell surface glycoproteins	553:578	cell surface glycoproteins	553:578	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	3	8	theme	cell	695:698	arg1	wall					700:703	the cell wall	691:703	the cell wall	691:703	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	1	9	theme	precise	179:185	arg1	mechanisms					187:196	the precise mechanisms	175:196	the precise mechanisms by which they operate	175:218	Cell surface glycoproteins in plants were first described more than 50 years ago, and yet, the precise mechanisms by which they operate remain elusive to this day.					
32896318	10	10	theme	glycoproteins	1746:1758	arg1	handling					1728:1735	the handling	1724:1735	the handling of plant glycoproteins	1724:1758	Throughout the chapter, we provide recommendations for the handling of plant glycoproteins and highlight special considerations for experimental design, along with troubleshooting suggestions.					
32896318	4	11	theme	glycoprotein	831:842	arg1	status					797:802	the glycosylation status	779:802	the glycosylation status of a presumed cell surface glycoprotein	779:842	In this chapter, we guide the reader through a protocol aimed to address the glycosylation status of a presumed cell surface glycoprotein.					
32896318	6	12	theme	partial	1054:1060	arg1	purification					1062:1073	partial purification	1054:1073	partial purification	1054:1073	Next, we describe a protocol for microsomal enrichment, followed by partial purification by affinity chromatography and finally glycodetection by immunoblotting using monoclonal antibodies targeting cell wall glycans.					
32896318	3	13	theme	cell	553:556	arg1	glycoproteins					566:578	cell surface glycoproteins	553:578	cell surface glycoproteins	553:578	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	7	14	theme	cell	1336:1339	arg1	wall					1341:1344	the plant cell wall	1326:1344	the plant cell wall	1326:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	0	15	gly	glycoproteins	57:69	arg1	glycoproteins					57:69	cell surface glycoproteins	44:69	cell surface glycoproteins	44:69	Partial purification and immunodetection of cell surface glycoproteins from plants.					
32896318	2	16	theme	protein	469:475	arg1	structure					477:485	protein structure	469:485	protein structure	469:485	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	6	17	theme	cell	1185:1188	arg1	glycans					1195:1201	cell wall glycans	1185:1201	cell wall glycans	1185:1201	Next, we describe a protocol for microsomal enrichment, followed by partial purification by affinity chromatography and finally glycodetection by immunoblotting using monoclonal antibodies targeting cell wall glycans.					
32896318	4	18	gly	glycoprotein	831:842	arg1	glycoprotein					831:842	a presumed cell surface glycoprotein	807:842	a presumed cell surface glycoprotein	807:842	In this chapter, we guide the reader through a protocol aimed to address the glycosylation status of a presumed cell surface glycoprotein.					
32896318	5	19	dep	advantages	867:876	arg1	the					863:865	the	863:865	the	863:865	First, we discuss the advantages and disadvantages of using plants as homologous expression systems for recombinant glycoprotein production.					
32896318	8	20	theme	-like	1476:1480	arg1	protein					1482:1488	an arabinogalactan protein (AGP)-like protein	1444:1488	the second an arabinogalactan protein (AGP)-like protein	1433:1488	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	7	21	gly	glycoproteins	1309:1321	arg1	glycoproteins					1309:1321	glycoproteins	1309:1321	glycoproteins in the plant cell wall	1309:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	1	22	theme	Cell	84:87	arg1	glycoproteins					97:109	Cell surface glycoproteins	84:109	Cell surface glycoproteins in plants	84:119	Cell surface glycoproteins in plants were first described more than 50 years ago, and yet, the precise mechanisms by which they operate remain elusive to this day.					
32896318	6	23	theme	microsomal	1019:1028	arg1	enrichment					1030:1039	microsomal enrichment	1019:1039	microsomal enrichment	1019:1039	Next, we describe a protocol for microsomal enrichment, followed by partial purification by affinity chromatography and finally glycodetection by immunoblotting using monoclonal antibodies targeting cell wall glycans.					
32896318	0	24	theme	Partial	0:6	arg1	purification					8:19	Partial purification	0:19	Partial purification	0:19	Partial purification and immunodetection of cell surface glycoproteins from plants.					
32896318	2	25	theme	present	401:407	arg1	localization					317:328	their subcellular localization	299:328	their subcellular localization (many secreted or membrane associated)	299:367	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	2	25	theme	present	401:407	arg1	extent					377:382	the extent	373:382	the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior	373:498	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	8	26	dep	second	1437:1442	arg1	protein					1482:1488	an arabinogalactan protein (AGP)-like protein	1444:1488	the second an arabinogalactan protein (AGP)-like protein	1433:1488	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	7	27	theme	abundant	1290:1297	arg1	family					1299:1304	the most abundant family	1281:1304	the most abundant family of glycoproteins in the plant cell wall	1281:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	7	27	theme	abundant	1290:1297	arg1	family					1273:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	9	28	with	conjunction	1628:1638	arg1	protocol					1659:1666	the described protocol	1645:1666	the described protocol	1645:1666	For the latter, we provide an AGP-specific protocol to ensure enrichment of members of this group, which can be used independently or in conjunction with the described protocol.					
32896318	10	29	theme	experimental	1801:1812	arg1	design					1814:1819	experimental design	1801:1819	experimental design	1801:1819	Throughout the chapter, we provide recommendations for the handling of plant glycoproteins and highlight special considerations for experimental design, along with troubleshooting suggestions.					
32896318	8	30	theme	HRGP	1383:1386	arg1	proteins					1397:1404	two putative HRGP chimeric proteins	1370:1404	two putative HRGP chimeric proteins	1370:1404	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	8	30	theme	HRGP	1383:1386	arg1	one					1407:1409	one	1407:1409	one	1407:1409	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	8	30	theme	HRGP	1383:1386	arg1	second					1437:1442	second	1437:1442	second	1437:1442	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	0	31	theme	surface	49:55	arg1	glycoproteins					57:69	cell surface glycoproteins	44:69	cell surface glycoproteins	44:69	Partial purification and immunodetection of cell surface glycoproteins from plants.					
32896318	6	32	theme	affinity	1078:1085	arg1	chromatography					1087:1100	affinity chromatography	1078:1100	affinity chromatography	1078:1100	Next, we describe a protocol for microsomal enrichment, followed by partial purification by affinity chromatography and finally glycodetection by immunoblotting using monoclonal antibodies targeting cell wall glycans.					
32896318	3	33	theme	direct	631:636	arg1	linkages					638:645	direct linkages	631:645	direct linkages with the polysaccharide networks present in the cell wall	631:703	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	8	34	theme	proteins	1397:1404	arg1	examples					1358:1365	examples	1358:1365	examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein	1358:1488	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	2	35	theme	subcellular	305:315	arg1	localization					317:328	their subcellular localization	299:328	their subcellular localization (many secreted or membrane associated)	299:367	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	7	36	from	family	1299:1304	arg1	wall					1341:1344	the plant cell wall	1326:1344	the plant cell wall	1326:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	7	37	theme	HRGP	1267:1270	arg1	family					1299:1304	the most abundant family	1281:1304	the most abundant family of glycoproteins in the plant cell wall	1281:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	7	37	theme	HRGP	1267:1270	arg1	family					1273:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	1	38	from	glycoproteins	97:109	arg1	plants					114:119	plants	114:119	plants	114:119	Cell surface glycoproteins in plants were first described more than 50 years ago, and yet, the precise mechanisms by which they operate remain elusive to this day.					
32896318	5	39	theme	recombinant	949:959	arg1	production					974:983	recombinant glycoprotein production	949:983	recombinant glycoprotein production	949:983	First, we discuss the advantages and disadvantages of using plants as homologous expression systems for recombinant glycoprotein production.					
32896318	7	40	from	glycoproteins	1309:1321	arg1	wall					1341:1344	the plant cell wall	1326:1344	the plant cell wall	1326:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	7	41	theme	glycoprotein	1253:1264	arg1	family					1299:1304	the most abundant family	1281:1304	the most abundant family of glycoproteins in the plant cell wall	1281:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	7	41	theme	glycoprotein	1253:1264	arg1	family					1273:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	3	42	theme	polysaccharide	656:669	arg1	networks					671:678	the polysaccharide networks	652:678	the polysaccharide networks present in the cell wall	652:703	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	10	43	gly	glycoproteins	1746:1758	arg1	glycoproteins					1746:1758	plant glycoproteins	1740:1758	plant glycoproteins	1740:1758	Throughout the chapter, we provide recommendations for the handling of plant glycoproteins and highlight special considerations for experimental design, along with troubleshooting suggestions.					
32896318	2	44	gly	glycoproteins	257:269	arg1	glycoproteins					257:269	glycoproteins	257:269	glycoproteins	257:269	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	4	45	theme	presumed	809:816	arg1	glycoprotein					831:842	a presumed cell surface glycoprotein	807:842	a presumed cell surface glycoprotein	807:842	In this chapter, we guide the reader through a protocol aimed to address the glycosylation status of a presumed cell surface glycoprotein.					
32896318	6	46	theme	monoclonal	1153:1162	arg1	antibodies					1164:1173	monoclonal antibodies	1153:1173	monoclonal antibodies targeting cell wall glycans	1153:1201	Next, we describe a protocol for microsomal enrichment, followed by partial purification by affinity chromatography and finally glycodetection by immunoblotting using monoclonal antibodies targeting cell wall glycans.					
32896318	3	47	theme	present	680:686	arg1	networks					671:678	the polysaccharide networks	652:678	the polysaccharide networks present in the cell wall	652:703	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	3	48	theme	complexity	533:542	arg1	layers					523:528	additional layers	512:528	additional layers of complexity	512:542	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	4	49	theme	glycosylation	783:795	arg1	status					797:802	the glycosylation status	779:802	the glycosylation status of a presumed cell surface glycoprotein	779:842	In this chapter, we guide the reader through a protocol aimed to address the glycosylation status of a presumed cell surface glycoprotein.					
32896318	2	50	contain	have	444:447	arg1	backbone					424:431	the protein backbone	412:431	the protein backbone	412:431	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	2	50	contain	have	444:447	arg2	effects					458:464	profound effects	449:464	profound effects	449:464	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	3	51	theme	surface	558:564	arg1	glycoproteins					566:578	cell surface glycoproteins	553:578	cell surface glycoproteins	553:578	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	4	52	gly	glycosylation	783:795	arg1	glycoprotein					831:842	a presumed cell surface glycoprotein	807:842	a presumed cell surface glycoprotein	807:842	In this chapter, we guide the reader through a protocol aimed to address the glycosylation status of a presumed cell surface glycoprotein.					
32896318	9	53	theme	described	1649:1657	arg1	protocol					1659:1666	the described protocol	1645:1666	the described protocol	1645:1666	For the latter, we provide an AGP-specific protocol to ensure enrichment of members of this group, which can be used independently or in conjunction with the described protocol.					
32896318	0	54	from	plants	76:81	arg1	immunodetection					25:39	immunodetection	25:39	immunodetection	25:39	Partial purification and immunodetection of cell surface glycoproteins from plants.					
32896318	0	54	from	plants	76:81	arg1	purification					8:19	Partial purification	0:19	Partial purification	0:19	Partial purification and immunodetection of cell surface glycoproteins from plants.					
32896318	10	55	theme	plant	1740:1744	arg1	glycoproteins					1746:1758	plant glycoproteins	1740:1758	plant glycoproteins	1740:1758	Throughout the chapter, we provide recommendations for the handling of plant glycoproteins and highlight special considerations for experimental design, along with troubleshooting suggestions.					
32896318	4	56	theme	surface	823:829	arg1	glycoprotein					831:842	a presumed cell surface glycoprotein	807:842	a presumed cell surface glycoprotein	807:842	In this chapter, we guide the reader through a protocol aimed to address the glycosylation status of a presumed cell surface glycoprotein.					
32896318	5	57	theme	expression	926:935	arg1	plants					905:910	plants	905:910	plants	905:910	First, we discuss the advantages and disadvantages of using plants as homologous expression systems for recombinant glycoprotein production.					
32896318	5	57	theme	expression	926:935	arg1	systems					937:943	homologous expression systems	915:943	homologous expression systems for recombinant glycoprotein production	915:983	First, we discuss the advantages and disadvantages of using plants as homologous expression systems for recombinant glycoprotein production.					
32896318	3	58	theme	close	587:591	arg1	contact					593:599	close contact	587:599	close contact	587:599	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	6	59	theme	wall	1190:1193	arg1	glycans					1195:1201	cell wall glycans	1185:1201	cell wall glycans	1185:1201	Next, we describe a protocol for microsomal enrichment, followed by partial purification by affinity chromatography and finally glycodetection by immunoblotting using monoclonal antibodies targeting cell wall glycans.					
32896318	10	60	theme	special	1774:1780	arg1	considerations					1782:1795	special considerations	1774:1795	special considerations for experimental design	1774:1819	Throughout the chapter, we provide recommendations for the handling of plant glycoproteins and highlight special considerations for experimental design, along with troubleshooting suggestions.					
32896318	7	61	theme	plant	1330:1334	arg1	wall					1341:1344	the plant cell wall	1326:1344	the plant cell wall	1326:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	2	62	theme	profound	449:456	arg1	effects					458:464	profound effects	449:464	profound effects	449:464	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	2	63	from	backbone	424:431	arg1	localization					317:328	their subcellular localization	299:328	their subcellular localization (many secreted or membrane associated)	299:367	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	2	63	from	backbone	424:431	arg1	extent					377:382	the extent	373:382	the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior	373:498	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	1	64	gly	glycoproteins	97:109	arg1	glycoproteins					97:109	Cell surface glycoproteins	84:109	Cell surface glycoproteins in plants	84:119	Cell surface glycoproteins in plants were first described more than 50 years ago, and yet, the precise mechanisms by which they operate remain elusive to this day.					
32896318	7	65	theme	glycoproteins	1309:1321	arg1	family					1299:1304	the most abundant family	1281:1304	the most abundant family of glycoproteins in the plant cell wall	1281:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	7	65	theme	glycoproteins	1309:1321	arg1	family					1273:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	3	66	from	wall	700:703	arg1	present					680:686	present	680:686	present	680:686	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
32896318	1	67	theme	surface	89:95	arg1	glycoproteins					97:109	Cell surface glycoproteins	84:109	Cell surface glycoproteins in plants	84:119	Cell surface glycoproteins in plants were first described more than 50 years ago, and yet, the precise mechanisms by which they operate remain elusive to this day.					
32896318	7	68	from	wall	1341:1344	arg1	family					1299:1304	the most abundant family	1281:1304	the most abundant family of glycoproteins in the plant cell wall	1281:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	7	68	from	wall	1341:1344	arg1	family					1273:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	2	69	theme	protein	416:422	arg1	backbone					424:431	the protein backbone	412:431	the protein backbone	412:431	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	7	70	gly	glycoprotein	1253:1264	arg1	glycoprotein					1253:1264	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	9	71	theme	AGP-specific	1521:1532	arg1	protocol					1534:1541	an AGP-specific protocol	1518:1541	an AGP-specific protocol to ensure enrichment of members of this group, which can be used independently or in conjunction with the described protocol	1518:1666	For the latter, we provide an AGP-specific protocol to ensure enrichment of members of this group, which can be used independently or in conjunction with the described protocol.					
32896318	2	72	dep	localization	317:328	arg1	secreted					336:343	secreted	336:343	secreted	336:343	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	2	72	dep	localization	317:328	arg1	associated					357:366	associated	357:366	associated	357:366	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	2	73	theme	glycosylation	387:399	arg1	present					401:407	glycosylation present	387:407	glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior	387:498	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	0	74	theme	cell	44:47	arg1	glycoproteins					57:69	cell surface glycoproteins	44:69	cell surface glycoproteins	44:69	Partial purification and immunodetection of cell surface glycoproteins from plants.					
32896318	5	75	theme	glycoprotein	961:972	arg1	production					974:983	recombinant glycoprotein production	949:983	recombinant glycoprotein production	949:983	First, we discuss the advantages and disadvantages of using plants as homologous expression systems for recombinant glycoprotein production.					
32896318	0	76	theme	glycoproteins	57:69	arg1	immunodetection					25:39	immunodetection	25:39	immunodetection	25:39	Partial purification and immunodetection of cell surface glycoproteins from plants.					
32896318	0	76	theme	glycoproteins	57:69	arg1	purification					8:19	Partial purification	0:19	Partial purification	0:19	Partial purification and immunodetection of cell surface glycoproteins from plants.					
32896318	8	77	theme	putative	1374:1381	arg1	proteins					1397:1404	two putative HRGP chimeric proteins	1370:1404	two putative HRGP chimeric proteins	1370:1404	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	8	77	theme	putative	1374:1381	arg1	one					1407:1409	one	1407:1409	one	1407:1409	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	8	77	theme	putative	1374:1381	arg1	second					1437:1442	second	1437:1442	second	1437:1442	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	9	78	theme	group	1583:1587	arg1	members					1567:1573	members	1567:1573	members of this group	1567:1587	For the latter, we provide an AGP-specific protocol to ensure enrichment of members of this group, which can be used independently or in conjunction with the described protocol.					
32896318	9	79	theme	members	1567:1573	arg1	enrichment					1553:1562	enrichment	1553:1562	enrichment	1553:1562	For the latter, we provide an AGP-specific protocol to ensure enrichment of members of this group, which can be used independently or in conjunction with the described protocol.					
32896318	2	80	from	present	401:407	arg1	backbone					424:431	the protein backbone	412:431	the protein backbone	412:431	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	5	81	theme	homologous	915:924	arg1	plants					905:910	plants	905:910	plants	905:910	First, we discuss the advantages and disadvantages of using plants as homologous expression systems for recombinant glycoprotein production.					
32896318	5	81	theme	homologous	915:924	arg1	systems					937:943	homologous expression systems	915:943	homologous expression systems for recombinant glycoprotein production	915:983	First, we discuss the advantages and disadvantages of using plants as homologous expression systems for recombinant glycoprotein production.					
32896318	10	82	theme	troubleshooting	1833:1847	arg1	suggestions					1849:1859	troubleshooting suggestions	1833:1859	troubleshooting suggestions	1833:1859	Throughout the chapter, we provide recommendations for the handling of plant glycoproteins and highlight special considerations for experimental design, along with troubleshooting suggestions.					
32896318	8	83	theme	chimeric	1388:1395	arg1	proteins					1397:1404	two putative HRGP chimeric proteins	1370:1404	two putative HRGP chimeric proteins	1370:1404	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	8	83	theme	chimeric	1388:1395	arg1	one					1407:1409	one	1407:1409	one	1407:1409	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	8	83	theme	chimeric	1388:1395	arg1	second					1437:1442	second	1437:1442	second	1437:1442	We provide examples of two putative HRGP chimeric proteins, one akin to extensins and the second an arabinogalactan protein (AGP)-like protein.					
32896318	2	84	from	extent	377:382	arg1	backbone					424:431	the protein backbone	412:431	the protein backbone	412:431	Studying glycoproteins is often challenging due to their subcellular localization (many secreted or membrane associated) and the extent of glycosylation present on the protein backbone, which can have profound effects on protein structure and behavior.					
32896318	7	85	theme	hydroxyproline-rich	1233:1251	arg1	family					1299:1304	the most abundant family	1281:1304	the most abundant family of glycoproteins in the plant cell wall	1281:1344	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	7	85	theme	hydroxyproline-rich	1233:1251	arg1	family					1273:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	the hydroxyproline-rich glycoprotein (HRGP) family	1229:1278	We particularly focus on the hydroxyproline-rich glycoprotein (HRGP) family, the most abundant family of glycoproteins in the plant cell wall.					
32896318	3	86	with	linkages	638:645	arg1	networks					671:678	the polysaccharide networks	652:678	the polysaccharide networks present in the cell wall	652:703	In plants, additional layers of complexity exist as cell surface glycoproteins are in close contact, and in some cases, establish direct linkages with the polysaccharide networks present in the cell wall.					
34278777	6	0	theme	positive	769:776	arg1	influence					778:786	the positive influence	765:786	the positive influence of collagen on cell growth	765:813	In the unassembled scaffolds, the positive influence of collagen on cell growth mostly worn out in 48 h, while the addition of chitin enhanced this effect for over 72 h.					
34278777	1	1	theme	biocompatible	156:168	arg1	route					170:174	A green biocompatible route	148:174	A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites	148:267	A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites was proposed.					
34278777	5	2	theme	assembly	725:732	arg1	influence					696:704	the influence	692:704	the influence of composition and assembly	692:732	The sets were tested as scaffolds for fibroblast growth (NIH-3T3) to study the influence of composition and assembly.					
34278777	2	3	with	samples	314:320	arg1	ratios					353:358	different collagen/chitin ratios	327:358	different collagen/chitin ratios	327:358	Both assembled and unassembled samples with different collagen/chitin ratios were synthesized, maintaining the β-chitin polymorph.					
34278777	4	4	theme	collagen	569:576	arg1	nanoaggregates					578:591	collagen nanoaggregates	569:591	collagen nanoaggregates	569:591	The second set presented a nanohomogeneous composition based on collagen nanoaggregates and chitin nanofibrils.					
34278777	5	5	theme	fibroblast	655:664	arg1	growth					666:671	fibroblast growth	655:671	fibroblast growth (NIH-3T3)	655:681	The sets were tested as scaffolds for fibroblast growth (NIH-3T3) to study the influence of composition and assembly.					
34278777	5	5	theme	fibroblast	655:664	arg1	NIH-3T3					674:680	NIH-3T3	674:680	NIH-3T3	674:680	The sets were tested as scaffolds for fibroblast growth (NIH-3T3) to study the influence of composition and assembly.					
34278777	1	6	theme	composites	258:267	arg1	deposition					184:193	deposition	184:193	deposition	184:193	A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites was proposed.					
34278777	1	6	theme	composites	258:267	arg1	assembly					212:219	simultaneous assembly	199:219	simultaneous assembly	199:219	A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites was proposed.					
34278777	8	7	theme	scaffold	1159:1166	arg1	design					1168:1173	scaffold design	1159:1173	scaffold design	1159:1173	This work highlighted critical aspects of the influence that composition and assembly has on fibroblast growth, a knowledge worth exploiting in scaffold design and preparation.					
34278777	5	8	theme	composition	709:719	arg1	influence					696:704	the influence	692:704	the influence of composition and assembly	692:732	The sets were tested as scaffolds for fibroblast growth (NIH-3T3) to study the influence of composition and assembly.					
34278777	7	9	theme	positive	970:977	arg1	effect					979:984	a less positive effect	963:984	a less positive effect on viability	963:997	The assembled samples showed higher viability at 24 h but a less positive effect on viability along the time.					
34278777	8	10	theme	critical	1037:1044	arg1	aspects					1046:1052	critical aspects	1037:1052	critical aspects of the influence that composition and assembly has on fibroblast growth, a knowledge worth exploiting in scaffold design and preparation	1037:1189	This work highlighted critical aspects of the influence that composition and assembly has on fibroblast growth, a knowledge worth exploiting in scaffold design and preparation.					
34278777	8	11	theme	fibroblast	1108:1117	arg1	growth					1119:1124	fibroblast growth	1108:1124	fibroblast growth	1108:1124	This work highlighted critical aspects of the influence that composition and assembly has on fibroblast growth, a knowledge worth exploiting in scaffold design and preparation.					
34278777	8	11	theme	fibroblast	1108:1117	arg1	knowledge					1129:1137	a knowledge	1127:1137	a knowledge worth exploiting in scaffold design and preparation	1127:1189	This work highlighted critical aspects of the influence that composition and assembly has on fibroblast growth, a knowledge worth exploiting in scaffold design and preparation.					
34278777	2	12	theme	assembled	288:296	arg1	samples					314:320	Both assembled and unassembled samples	283:320	Both assembled and unassembled samples with different collagen/chitin ratios	283:358	Both assembled and unassembled samples with different collagen/chitin ratios were synthesized, maintaining the β-chitin polymorph.					
34278777	7	13	from	h	957:957	arg1	viability					941:949	higher viability	934:949	higher viability at 24 h	934:957	The assembled samples showed higher viability at 24 h but a less positive effect on viability along the time.					
34278777	3	14	theme	compositional	468:480	arg1	homogeneity					492:502	compositional submicron homogeneity	468:502	compositional submicron homogeneity	468:502	The first set showed a microfibrous organization with compositional submicron homogeneity.					
34278777	0	15	theme	Assembly	106:113	arg1	Influence					115:123	Assembly Influence	106:123	Assembly Influence on Fibroblasts Growth	106:145	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.					
34278777	4	16	theme	nanohomogeneous	532:546	arg1	composition					548:558	a nanohomogeneous composition	530:558	a nanohomogeneous composition based on collagen nanoaggregates and chitin nanofibrils	530:614	The second set presented a nanohomogeneous composition based on collagen nanoaggregates and chitin nanofibrils.					
34278777	1	17	theme	collagen/chitin	242:256	arg1	composites					258:267	collagen/chitin composites	242:267	collagen/chitin composites	242:267	A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites was proposed.					
34278777	3	18	theme	first	418:422	arg1	set					424:426	The first set	414:426	The first set	414:426	The first set showed a microfibrous organization with compositional submicron homogeneity.					
34278777	3	19	theme	submicron	482:490	arg1	homogeneity					492:502	compositional submicron homogeneity	468:502	compositional submicron homogeneity	468:502	The first set showed a microfibrous organization with compositional submicron homogeneity.					
34278777	0	20	theme	Biocompatible	6:18	arg1	Method					20:25	Green Biocompatible Method	0:25	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.	0:146	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.					
34278777	2	21	theme	different	327:335	arg1	ratios					353:358	different collagen/chitin ratios	327:358	different collagen/chitin ratios	327:358	Both assembled and unassembled samples with different collagen/chitin ratios were synthesized, maintaining the β-chitin polymorph.					
34278777	6	22	theme	unassembled	742:752	arg1	scaffolds					754:762	the unassembled scaffolds	738:762	the unassembled scaffolds	738:762	In the unassembled scaffolds, the positive influence of collagen on cell growth mostly worn out in 48 h, while the addition of chitin enhanced this effect for over 72 h.					
34278777	0	23	theme	Green	0:4	arg1	Method					20:25	Green Biocompatible Method	0:25	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.	0:146	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.					
34278777	8	24	theme	influence	1061:1069	arg1	aspects					1046:1052	critical aspects	1037:1052	critical aspects of the influence that composition and assembly has on fibroblast growth, a knowledge worth exploiting in scaffold design and preparation	1037:1189	This work highlighted critical aspects of the influence that composition and assembly has on fibroblast growth, a knowledge worth exploiting in scaffold design and preparation.					
34278777	0	25	dep	Study	78:82	arg1	Composition					90:100	Their Composition	84:100	Their Composition	84:100	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.					
34278777	0	26	theme	Fibroblasts	128:138	arg1	Growth					140:145	Fibroblasts Growth	128:145	Fibroblasts Growth	128:145	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.					
34278777	1	27	theme	simultaneous	199:210	arg1	assembly					212:219	simultaneous assembly	199:219	simultaneous assembly	199:219	A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites was proposed.					
34278777	2	28	theme	unassembled	302:312	arg1	samples					314:320	Both assembled and unassembled samples	283:320	Both assembled and unassembled samples with different collagen/chitin ratios	283:358	Both assembled and unassembled samples with different collagen/chitin ratios were synthesized, maintaining the β-chitin polymorph.					
34278777	6	29	from	influence	778:786	arg1	growth					808:813	cell growth	803:813	cell growth	803:813	In the unassembled scaffolds, the positive influence of collagen on cell growth mostly worn out in 48 h, while the addition of chitin enhanced this effect for over 72 h.					
34278777	0	30	from	Study	78:82	arg1	Growth					140:145	Fibroblasts Growth	128:145	Fibroblasts Growth	128:145	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.					
34278777	1	31	dep	deposition	184:193	arg1	the					180:182	the	180:182	the	180:182	A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites was proposed.					
34278777	7	32	from	effect	979:984	arg1	viability					989:997	viability	989:997	viability	989:997	The assembled samples showed higher viability at 24 h but a less positive effect on viability along the time.					
34278777	3	33	theme	microfibrous	437:448	arg1	organization					450:461	a microfibrous organization	435:461	a microfibrous organization with compositional submicron homogeneity	435:502	The first set showed a microfibrous organization with compositional submicron homogeneity.					
34278777	4	34	theme	second	509:514	arg1	set					516:518	The second set	505:518	The second set	505:518	The second set presented a nanohomogeneous composition based on collagen nanoaggregates and chitin nanofibrils.					
34278777	0	35	theme	Collagen/Chitin	48:62	arg1	Composites					64:73	Collagen/Chitin Composites	48:73	Collagen/Chitin Composites	48:73	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.					
34278777	3	36	with	organization	450:461	arg1	homogeneity					492:502	compositional submicron homogeneity	468:502	compositional submicron homogeneity	468:502	The first set showed a microfibrous organization with compositional submicron homogeneity.					
34278777	0	37	from	Influence	115:123	arg1	Growth					140:145	Fibroblasts Growth	128:145	Fibroblasts Growth	128:145	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.					
34278777	6	38	theme	cell	803:806	arg1	growth					808:813	cell growth	803:813	cell growth	803:813	In the unassembled scaffolds, the positive influence of collagen on cell growth mostly worn out in 48 h, while the addition of chitin enhanced this effect for over 72 h.					
34278777	4	39	theme	chitin	597:602	arg1	nanofibrils					604:614	chitin nanofibrils	597:614	chitin nanofibrils	597:614	The second set presented a nanohomogeneous composition based on collagen nanoaggregates and chitin nanofibrils.					
34278777	6	40	theme	chitin	862:867	arg1	addition					850:857	the addition	846:857	the addition of chitin	846:867	In the unassembled scaffolds, the positive influence of collagen on cell growth mostly worn out in 48 h, while the addition of chitin enhanced this effect for over 72 h.					
34278777	7	41	theme	higher	934:939	arg1	viability					941:949	higher viability	934:949	higher viability at 24 h	934:957	The assembled samples showed higher viability at 24 h but a less positive effect on viability along the time.					
34278777	1	42	theme	pH	225:226	arg1	increment					228:236	pH increment	225:236	pH increment	225:236	A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites was proposed.					
34278777	6	43	theme	collagen	791:798	arg1	influence					778:786	the positive influence	765:786	the positive influence of collagen on cell growth	765:813	In the unassembled scaffolds, the positive influence of collagen on cell growth mostly worn out in 48 h, while the addition of chitin enhanced this effect for over 72 h.					
34278777	2	44	theme	collagen/chitin	337:351	arg1	ratios					353:358	different collagen/chitin ratios	327:358	different collagen/chitin ratios	327:358	Both assembled and unassembled samples with different collagen/chitin ratios were synthesized, maintaining the β-chitin polymorph.					
34278777	7	45	theme	assembled	909:917	arg1	samples					919:925	The assembled samples	905:925	The assembled samples	905:925	The assembled samples showed higher viability at 24 h but a less positive effect on viability along the time.					
34278777	2	46	theme	β-chitin	394:401	arg1	polymorph					403:411	the β-chitin polymorph	390:411	the β-chitin polymorph	390:411	Both assembled and unassembled samples with different collagen/chitin ratios were synthesized, maintaining the β-chitin polymorph.					
34278777	0	47	theme	Composites	64:73	arg1	Synthesis					35:43	the Synthesis	31:43	the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth	31:145	Green Biocompatible Method for the Synthesis of Collagen/Chitin Composites to Study Their Composition and Assembly Influence on Fibroblasts Growth.					
34278777	1	48	theme	green	150:154	arg1	route					170:174	A green biocompatible route	148:174	A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites	148:267	A green biocompatible route for the deposition and simultaneous assembly, by pH increment, of collagen/chitin composites was proposed.					
34051740	7	0	theme	adherent	1554:1561	arg1	mucilage					1585:1592	the adherent and non-adherent seed mucilage	1550:1592	the adherent and non-adherent seed mucilage	1550:1592	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	5	1	dep	RESULTS	869:875	arg1	characterized					891:903	characterized	891:903	have characterized two β-glucuronosyltransferase genes, GLCAT14A and GLCAT14C, that are involved in the transfer of β-glucuronic acid (GlcA) to type II AGs	886:1040	RESULTS Here, we have characterized two β-glucuronosyltransferase genes, GLCAT14A and GLCAT14C, that are involved in the transfer of β-glucuronic acid (GlcA) to type II AGs.					
34051740	6	2	theme	subtle	1124:1129	arg1	alterations					1131:1141	subtle alterations	1124:1141	subtle alterations in mucilage pectin homogalacturonan (HG)	1124:1182	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	3	3	from	understanding	534:546	arg1	development					654:664	seed coat epidermal cell development	629:664	seed coat epidermal cell development	629:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	3	3	from	understanding	534:546	arg1	formation					615:623	mucilage formation	606:623	mucilage formation	606:623	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	9	4	theme	glcat14a-1glcat14c-1	1713:1732	arg1	mutants					1734:1740	glcat14a-1glcat14c-1 mutants	1713:1740	glcat14a-1glcat14c-1 mutants	1713:1740	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	3	5	theme	glycans/sugar	580:592	arg1	residues					594:601	these glycans/sugar residues	574:601	these glycans/sugar residues	574:601	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	11	6	dep	matrix	2204:2209	arg1	the					2191:2193	the	2191:2193	the	2191:2193	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	10	7	theme	mucilage	2033:2040	arg1	formation					2042:2050	mucilage formation	2033:2050	mucilage formation	2033:2050	CONCLUSIONS These results raise important questions regarding cell wall polymer interactions and organization during mucilage formation.					
34051740	11	8	theme	GLCAT14C	2110:2117	arg1	activities					2083:2092	the enzymatic activities	2069:2092	the enzymatic activities of GLCAT14A and GLCAT14C	2069:2117	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	5	9	theme	β-glucuronosyltransferase	909:933	arg1	GLCAT14C					955:962	GLCAT14C	955:962	GLCAT14C	955:962	RESULTS Here, we have characterized two β-glucuronosyltransferase genes, GLCAT14A and GLCAT14C, that are involved in the transfer of β-glucuronic acid (GlcA) to type II AGs.					
34051740	5	9	theme	β-glucuronosyltransferase	909:933	arg1	GLCAT14A					942:949	GLCAT14A	942:949	GLCAT14A	942:949	RESULTS Here, we have characterized two β-glucuronosyltransferase genes, GLCAT14A and GLCAT14C, that are involved in the transfer of β-glucuronic acid (GlcA) to type II AGs.					
34051740	5	9	theme	β-glucuronosyltransferase	909:933	arg1	genes					935:939	two β-glucuronosyltransferase genes	905:939	two β-glucuronosyltransferase genes	905:939	RESULTS Here, we have characterized two β-glucuronosyltransferase genes, GLCAT14A and GLCAT14C, that are involved in the transfer of β-glucuronic acid (GlcA) to type II AGs.					
34051740	7	10	theme	mutants	1524:1530	arg1	amounts					1492:1498	the sugar amounts	1482:1498	the sugar amounts of glcat14a-1glcat14c-1 mutants	1482:1530	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	7	10	theme	mutants	1524:1530	arg1	mutants					1524:1530	glcat14a-1glcat14c-1 mutants	1503:1530	glcat14a-1glcat14c-1 mutants	1503:1530	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	11	11	theme	Arabidopsis	2228:2238	arg1	thaliana					2240:2247	Arabidopsis thaliana	2228:2247	Arabidopsis thaliana	2228:2247	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	4	12	theme	such	714:717	arg1	AGPs					768:771	AGPs	768:771	AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes	768:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	4	12	theme	such	714:717	arg1	residues					756:763	the glucuronic acid residues	736:763	the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes	736:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	4	12	theme	such	714:717	arg1	residue					725:731	One such sugar residue	710:731	One such sugar residue	710:731	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	6	13	theme	severe	1267:1272	arg1	phenotypes					1283:1292	much more severe mucilage phenotypes	1257:1292	much more severe mucilage phenotypes	1257:1292	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	13	theme	severe	1267:1272	arg1	loss					1305:1308	loss	1305:1308	loss of adherent mucilage	1305:1329	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	13	theme	severe	1267:1272	arg1	alterations					1347:1357	significant alterations	1335:1357	significant alterations in cellulose ray formation and seed coat morphology	1335:1409	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	9	14	theme	calcium	1778:1784	arg1	content					1786:1792	calcium content	1778:1792	calcium content	1778:1792	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	12	15	theme	potential	2304:2312	arg1	targets					2319:2325	potential gene targets	2304:2325	potential gene targets for engineering plant cell walls for industrial applications	2304:2386	This work brings us a step closer towards identifying potential gene targets for engineering plant cell walls for industrial applications.					
34051740	6	16	theme	ray	1372:1374	arg1	formation					1376:1384	cellulose ray formation	1362:1384	cellulose ray formation	1362:1384	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	1	17	theme	type	242:245	arg1	AG					267:268	AG	267:268	AG	267:268	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	1	17	theme	type	242:245	arg1	arabinogalactan					250:264	type II arabinogalactan	242:264	type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone	242:347	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	7	18	theme	composition	1427:1437	arg1	analysis					1439:1446	Monosaccharide composition analysis	1412:1446	Monosaccharide composition analysis	1412:1446	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	3	19	theme	epidermal	639:647	arg1	development					654:664	seed coat epidermal cell development	629:664	seed coat epidermal cell development	629:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	1	20	theme	arabinogalactan	250:264	arg1	polysaccharides					271:285	type II arabinogalactan (AG) polysaccharides	242:285	type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone	242:347	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	9	21	from	defects	1749:1755	arg1	content					1893:1899	crystalline cellulose content	1871:1899	crystalline cellulose content	1871:1899	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	21	from	defects	1749:1755	arg1	size					1910:1913	seed size	1905:1913	seed size	1905:1913	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	21	from	defects	1749:1755	arg1	formation					1767:1775	pectin formation	1760:1775	pectin formation	1760:1775	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	11	22	theme	mucilage	2195:2202	arg1	matrix					2204:2209	mucilage matrix	2195:2209	mucilage matrix	2195:2209	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	4	23	theme	acid	751:754	arg1	AGPs					768:771	AGPs	768:771	AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes	768:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	4	23	theme	acid	751:754	arg1	residues					756:763	the glucuronic acid residues	736:763	the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes	736:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	4	23	theme	acid	751:754	arg1	residue					725:731	One such sugar residue	710:731	One such sugar residue	710:731	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	11	24	from	size	2220:2223	arg1	thaliana					2240:2247	Arabidopsis thaliana	2228:2247	Arabidopsis thaliana	2228:2247	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	6	25	theme	significant	1335:1345	arg1	alterations					1347:1357	significant alterations	1335:1357	significant alterations in cellulose ray formation and seed coat morphology	1335:1409	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	3	26	theme	seed	629:632	arg1	development					654:664	seed coat epidermal cell development	629:664	seed coat epidermal cell development	629:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	9	27	theme	pectin	1812:1817	arg1	DM					1842:1843	DM	1842:1843	DM	1842:1843	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	27	theme	pectin	1812:1817	arg1	methyl-esterification					1819:1839	pectin methyl-esterification	1812:1839	pectin methyl-esterification (DM)	1812:1844	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	6	28	theme	glcat14a-1glcat14c-1	1218:1237	arg1	mutants					1239:1245	glcat14a-1glcat14c-1 mutants	1218:1245	glcat14a-1glcat14c-1 mutants	1218:1245	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	29	theme	mucilage	1322:1329	arg1	loss					1305:1308	loss	1305:1308	loss of adherent mucilage	1305:1329	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	29	theme	mucilage	1322:1329	arg1	alterations					1347:1357	significant alterations	1335:1357	significant alterations in cellulose ray formation and seed coat morphology	1335:1409	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	1	30	attach	attached	287:294	arg2	polysaccharides					271:285	type II arabinogalactan (AG) polysaccharides	242:285	type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone	242:347	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	1	30	attach	attached	287:294	arg1	residues					314:321	hydroxyproline residues	299:321	hydroxyproline residues in their protein backbone	299:347	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	7	31	from	alterations	1467:1477	arg1	amounts					1492:1498	the sugar amounts	1482:1498	the sugar amounts of glcat14a-1glcat14c-1 mutants	1482:1530	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	7	31	from	alterations	1467:1477	arg1	mutants					1524:1530	glcat14a-1glcat14c-1 mutants	1503:1530	glcat14a-1glcat14c-1 mutants	1503:1530	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	3	32	from	formation	615:623	arg1	understanding					534:546	our understanding	530:546	our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development	530:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	0	33	theme	II	68:69	arg1	arabinogalactans					71:86	type II arabinogalactans	63:86	type II arabinogalactans	63:86	Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana.					
34051740	11	34	theme	GLCAT14A	2097:2104	arg1	activities					2083:2092	the enzymatic activities	2069:2092	the enzymatic activities of GLCAT14A and GLCAT14C	2069:2117	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	4	35	theme	AGP	799:801	arg1	glycans					803:809	AGP glycans	799:809	AGP glycans	799:809	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	0	36	theme	mucilage	100:107	arg1	organization					131:142	mucilage polysaccharide matrix organization	100:142	mucilage polysaccharide matrix organization in Arabidopsis thaliana	100:166	Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana.					
34051740	9	37	from	content	1786:1792	arg1	content					1893:1899	crystalline cellulose content	1871:1899	crystalline cellulose content	1871:1899	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	37	from	content	1786:1792	arg1	size					1910:1913	seed size	1905:1913	seed size	1905:1913	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	37	from	content	1786:1792	arg1	formation					1767:1775	pectin formation	1760:1775	pectin formation	1760:1775	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	3	38	from	development	654:664	arg1	understanding					534:546	our understanding	530:546	our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development	530:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	11	39	theme	size	2220:2223	arg1	organization					2175:2186	the organization	2171:2186	the organization of the mucilage matrix and seed size in Arabidopsis thaliana	2171:2247	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	0	40	theme	matrix	124:129	arg1	organization					131:142	mucilage polysaccharide matrix organization	100:142	mucilage polysaccharide matrix organization in Arabidopsis thaliana	100:166	Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana.					
34051740	6	41	theme	glcat14a-1	1095:1104	arg1	mutants					1106:1112	glcat14a-1 mutants	1095:1112	glcat14a-1 mutants	1095:1112	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	9	42	theme	crystalline	1871:1881	arg1	content					1893:1899	crystalline cellulose content	1871:1899	crystalline cellulose content	1871:1899	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	12	43	theme	gene	2314:2317	arg1	targets					2319:2325	potential gene targets	2304:2325	potential gene targets for engineering plant cell walls for industrial applications	2304:2386	This work brings us a step closer towards identifying potential gene targets for engineering plant cell walls for industrial applications.					
34051740	11	44	theme	enzymatic	2073:2081	arg1	activities					2083:2092	the enzymatic activities	2069:2092	the enzymatic activities of GLCAT14A and GLCAT14C	2069:2117	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	3	45	theme	AGs	504:506	arg1	importance					482:491	the importance	478:491	the importance of type II AGs in plant development	478:527	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	1	46	theme	protein	332:338	arg1	backbone					340:347	their protein backbone	326:347	their protein backbone	326:347	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	9	47	from	reductions	1857:1866	arg1	content					1893:1899	crystalline cellulose content	1871:1899	crystalline cellulose content	1871:1899	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	47	from	reductions	1857:1866	arg1	size					1910:1913	seed size	1905:1913	seed size	1905:1913	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	47	from	reductions	1857:1866	arg1	formation					1767:1775	pectin formation	1760:1775	pectin formation	1760:1775	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	5	48	theme	β-glucuronic	1002:1013	arg1	GlcA					1021:1024	GlcA	1021:1024	GlcA	1021:1024	RESULTS Here, we have characterized two β-glucuronosyltransferase genes, GLCAT14A and GLCAT14C, that are involved in the transfer of β-glucuronic acid (GlcA) to type II AGs.					
34051740	5	48	theme	β-glucuronic	1002:1013	arg1	acid					1015:1018	β-glucuronic acid	1002:1018	β-glucuronic acid (GlcA)	1002:1025	RESULTS Here, we have characterized two β-glucuronosyltransferase genes, GLCAT14A and GLCAT14C, that are involved in the transfer of β-glucuronic acid (GlcA) to type II AGs.					
34051740	9	49	theme	seed	1905:1908	arg1	size					1910:1913	seed size	1905:1913	seed size	1905:1913	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	8	50	theme	mucilage	1622:1629	arg1	content					1631:1637	total mucilage content	1616:1637	total mucilage content	1616:1637	Also, a reduction in total mucilage content was observed in glcat14a-1glcat14c-1 mutants relative to WT.					
34051740	2	51	theme	cellular	450:457	arg1	functions					459:467	normal cellular functions	443:467	normal cellular functions	443:467	Type II AGs are necessary for plant growth and critically important for the establishment of normal cellular functions.					
34051740	3	52	theme	type	496:499	arg1	AGs					504:506	type II AGs	496:506	type II AGs	496:506	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	6	53	theme	genetics	1059:1066	arg1	approach					1068:1075	a reverse genetics approach	1049:1075	a reverse genetics approach	1049:1075	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	2	54	theme	plant	380:384	arg1	growth					386:391	plant growth	380:391	plant growth	380:391	Type II AGs are necessary for plant growth and critically important for the establishment of normal cellular functions.					
34051740	6	55	from	alterations	1347:1357	arg1	morphology					1400:1409	seed coat morphology	1390:1409	seed coat morphology	1390:1409	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	55	from	alterations	1347:1357	arg1	formation					1376:1384	cellulose ray formation	1362:1384	cellulose ray formation	1362:1384	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	7	56	theme	seed	1580:1583	arg1	mucilage					1585:1592	the adherent and non-adherent seed mucilage	1550:1592	the adherent and non-adherent seed mucilage	1550:1592	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	10	57	theme	cell	1978:1981	arg1	interactions					1996:2007	cell wall polymer interactions	1978:2007	cell wall polymer interactions	1978:2007	CONCLUSIONS These results raise important questions regarding cell wall polymer interactions and organization during mucilage formation.					
34051740	3	58	theme	plant	511:515	arg1	development					517:527	plant development	511:527	plant development	511:527	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	6	59	from	loss	1305:1308	arg1	morphology					1400:1409	seed coat morphology	1390:1409	seed coat morphology	1390:1409	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	59	from	loss	1305:1308	arg1	formation					1376:1384	cellulose ray formation	1362:1384	cellulose ray formation	1362:1384	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	60	theme	wild	1196:1199	arg1	type					1201:1204	wild type	1196:1204	wild type (WT)	1196:1209	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	60	theme	wild	1196:1199	arg1	WT					1207:1208	WT	1207:1208	WT	1207:1208	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	5	61	theme	type	1030:1033	arg1	AGs					1038:1040	type II AGs	1030:1040	type II AGs	1030:1040	RESULTS Here, we have characterized two β-glucuronosyltransferase genes, GLCAT14A and GLCAT14C, that are involved in the transfer of β-glucuronic acid (GlcA) to type II AGs.					
34051740	6	62	theme	mucilage	1146:1153	arg1	HG					1180:1181	HG	1180:1181	HG	1180:1181	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	62	theme	mucilage	1146:1153	arg1	homogalacturonan					1162:1177	mucilage pectin homogalacturonan	1146:1177	mucilage pectin homogalacturonan (HG)	1146:1182	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	4	63	theme	β-glucuronosyltransferase	828:852	arg1	genes/enzymes					854:866	β-glucuronosyltransferase genes/enzymes	828:866	β-glucuronosyltransferase genes/enzymes	828:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	11	64	from	matrix	2204:2209	arg1	thaliana					2240:2247	Arabidopsis thaliana	2228:2247	Arabidopsis thaliana	2228:2247	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	6	65	theme	adherent	1313:1320	arg1	mucilage					1322:1329	adherent mucilage	1313:1329	adherent mucilage	1313:1329	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	66	from	alterations	1131:1141	arg1	HG					1180:1181	HG	1180:1181	HG	1180:1181	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	66	from	alterations	1131:1141	arg1	homogalacturonan					1162:1177	mucilage pectin homogalacturonan	1146:1177	mucilage pectin homogalacturonan (HG)	1146:1182	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	10	67	theme	polymer	1988:1994	arg1	interactions					1996:2007	cell wall polymer interactions	1978:2007	cell wall polymer interactions	1978:2007	CONCLUSIONS These results raise important questions regarding cell wall polymer interactions and organization during mucilage formation.					
34051740	1	68	gly	glycosylated	224:235	arg1	Arabinogalactan-proteins					180:203	BACKGROUND Arabinogalactan-proteins	169:203	BACKGROUND Arabinogalactan-proteins (AGPs)	169:210	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	1	68	gly	glycosylated	224:235	arg1	AGPs					206:209	AGPs	206:209	AGPs	206:209	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	1	69	theme	hydroxyproline	299:312	arg1	residues					314:321	hydroxyproline residues	299:321	hydroxyproline residues in their protein backbone	299:347	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	11	70	theme	seed	2215:2218	arg1	size					2220:2223	seed size	2215:2223	seed size	2215:2223	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	3	71	theme	role	566:569	arg1	understanding					534:546	our understanding	530:546	our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development	530:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	10	72	dep	CONCLUSIONS	1916:1926	arg1	raise					1942:1946	raise	1942:1946	raise important questions regarding cell wall polymer interactions and organization during mucilage formation	1942:2050	CONCLUSIONS These results raise important questions regarding cell wall polymer interactions and organization during mucilage formation.					
34051740	7	73	theme	glcat14a-1glcat14c-1	1503:1522	arg1	mutants					1524:1530	glcat14a-1glcat14c-1 mutants	1503:1530	glcat14a-1glcat14c-1 mutants	1503:1530	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	9	74	theme	pectin	1760:1765	arg1	formation					1767:1775	pectin formation	1760:1775	pectin formation	1760:1775	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	7	75	theme	sugar	1486:1490	arg1	amounts					1492:1498	the sugar amounts	1482:1498	the sugar amounts of glcat14a-1glcat14c-1 mutants	1482:1530	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	7	75	theme	sugar	1486:1490	arg1	mutants					1524:1530	glcat14a-1glcat14c-1 mutants	1503:1530	glcat14a-1glcat14c-1 mutants	1503:1530	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	6	76	theme	mucilage	1274:1281	arg1	phenotypes					1283:1292	much more severe mucilage phenotypes	1257:1292	much more severe mucilage phenotypes	1257:1292	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	76	theme	mucilage	1274:1281	arg1	loss					1305:1308	loss	1305:1308	loss of adherent mucilage	1305:1329	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	76	theme	mucilage	1274:1281	arg1	alterations					1347:1357	significant alterations	1335:1357	significant alterations in cellulose ray formation and seed coat morphology	1335:1409	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	3	77	theme	mucilage	606:613	arg1	formation					615:623	mucilage formation	606:623	mucilage formation	606:623	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	11	78	from	thaliana	2240:2247	arg1	organization					2175:2186	the organization	2171:2186	the organization of the mucilage matrix and seed size in Arabidopsis thaliana	2171:2247	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	0	79	theme	Arabidopsis	147:157	arg1	thaliana					159:166	Arabidopsis thaliana	147:166	Arabidopsis thaliana	147:166	Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana.					
34051740	2	80	theme	Type	350:353	arg1	AGs					358:360	Type II AGs	350:360	Type II AGs	350:360	Type II AGs are necessary for plant growth and critically important for the establishment of normal cellular functions.					
34051740	7	81	theme	significant	1455:1465	arg1	alterations					1467:1477	significant alterations	1455:1477	significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants	1455:1530	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	6	82	theme	cellulose	1362:1370	arg1	formation					1376:1384	cellulose ray formation	1362:1384	cellulose ray formation	1362:1384	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	3	83	theme	coat	634:637	arg1	development					654:664	seed coat epidermal cell development	629:664	seed coat epidermal cell development	629:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	11	84	theme	matrix	2204:2209	arg1	organization					2175:2186	the organization	2171:2186	the organization of the mucilage matrix and seed size in Arabidopsis thaliana	2171:2247	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	0	85	from	organization	131:142	arg1	thaliana					159:166	Arabidopsis thaliana	147:166	Arabidopsis thaliana	147:166	Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana.					
34051740	7	86	theme	Monosaccharide	1412:1425	arg1	analysis					1439:1446	Monosaccharide composition analysis	1412:1446	Monosaccharide composition analysis	1412:1446	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	4	87	theme	glucuronic	740:749	arg1	AGPs					768:771	AGPs	768:771	AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes	768:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	4	87	theme	glucuronic	740:749	arg1	residues					756:763	the glucuronic acid residues	736:763	the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes	736:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	4	87	theme	glucuronic	740:749	arg1	residue					725:731	One such sugar residue	710:731	One such sugar residue	710:731	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	3	88	theme	residues	594:601	arg1	role					566:569	the underlying role	551:569	the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development	551:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	6	89	theme	reverse	1051:1057	arg1	approach					1068:1075	a reverse genetics approach	1049:1075	a reverse genetics approach	1049:1075	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	9	90	theme	methyl-esterification	1819:1839	arg1	defects					1749:1755	defects	1749:1755	defects in pectin formation	1749:1775	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	90	theme	methyl-esterification	1819:1839	arg1	reductions					1857:1866	reductions	1857:1866	reductions in crystalline cellulose content and seed size	1857:1913	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	90	theme	methyl-esterification	1819:1839	arg1	content					1786:1792	calcium content	1778:1792	calcium content	1778:1792	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	90	theme	methyl-esterification	1819:1839	arg1	degree					1802:1807	the degree	1798:1807	the degree of pectin methyl-esterification (DM)	1798:1844	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	12	91	theme	plant	2343:2347	arg1	walls					2354:2358	plant cell walls	2343:2358	plant cell walls for industrial applications	2343:2386	This work brings us a step closer towards identifying potential gene targets for engineering plant cell walls for industrial applications.					
34051740	0	92	theme	arabinogalactans	71:86	arg1	biosynthesis					47:58	the biosynthesis	43:58	the biosynthesis of type II arabinogalactans	43:86	Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana.					
34051740	3	93	theme	cell	649:652	arg1	development					654:664	seed coat epidermal cell development	629:664	seed coat epidermal cell development	629:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	1	94	theme	BACKGROUND	169:178	arg1	Arabinogalactan-proteins					180:203	BACKGROUND Arabinogalactan-proteins	169:203	BACKGROUND Arabinogalactan-proteins (AGPs)	169:210	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	1	94	theme	BACKGROUND	169:178	arg1	AGPs					206:209	AGPs	206:209	AGPs	206:209	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	9	95	from	degree	1802:1807	arg1	content					1893:1899	crystalline cellulose content	1871:1899	crystalline cellulose content	1871:1899	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	95	from	degree	1802:1807	arg1	size					1910:1913	seed size	1905:1913	seed size	1905:1913	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	9	95	from	degree	1802:1807	arg1	formation					1767:1775	pectin formation	1760:1775	pectin formation	1760:1775	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	4	96	theme	sugar	719:723	arg1	AGPs					768:771	AGPs	768:771	AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes	768:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	4	96	theme	sugar	719:723	arg1	residues					756:763	the glucuronic acid residues	736:763	the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes	736:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	4	96	theme	sugar	719:723	arg1	residue					725:731	One such sugar residue	710:731	One such sugar residue	710:731	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	3	97	from	role	566:569	arg1	development					654:664	seed coat epidermal cell development	629:664	seed coat epidermal cell development	629:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	3	97	from	role	566:569	arg1	formation					615:623	mucilage formation	606:623	mucilage formation	606:623	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	6	98	theme	coat	1395:1398	arg1	morphology					1400:1409	seed coat morphology	1390:1409	seed coat morphology	1390:1409	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	1	99	from	residues	314:321	arg1	backbone					340:347	their protein backbone	326:347	their protein backbone	326:347	BACKGROUND Arabinogalactan-proteins (AGPs) are heavily glycosylated with type II arabinogalactan (AG) polysaccharides attached to hydroxyproline residues in their protein backbone.					
34051740	0	100	theme	polysaccharide	109:122	arg1	organization					131:142	mucilage polysaccharide matrix organization	100:142	mucilage polysaccharide matrix organization in Arabidopsis thaliana	100:166	Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana.					
34051740	5	101	theme	acid	1015:1018	arg1	transfer					990:997	the transfer	986:997	the transfer of β-glucuronic acid (GlcA) to type II AGs	986:1040	RESULTS Here, we have characterized two β-glucuronosyltransferase genes, GLCAT14A and GLCAT14C, that are involved in the transfer of β-glucuronic acid (GlcA) to type II AGs.					
34051740	12	102	theme	industrial	2364:2373	arg1	applications					2375:2386	industrial applications	2364:2386	industrial applications	2364:2386	This work brings us a step closer towards identifying potential gene targets for engineering plant cell walls for industrial applications.					
34051740	11	103	from	organization	2175:2186	arg1	thaliana					2240:2247	Arabidopsis thaliana	2228:2247	Arabidopsis thaliana	2228:2247	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	6	104	theme	seed	1390:1393	arg1	morphology					1400:1409	seed coat morphology	1390:1409	seed coat morphology	1390:1409	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	12	105	theme	cell	2349:2352	arg1	walls					2354:2358	plant cell walls	2343:2358	plant cell walls for industrial applications	2343:2386	This work brings us a step closer towards identifying potential gene targets for engineering plant cell walls for industrial applications.					
34051740	9	106	theme	cellulose	1883:1891	arg1	content					1893:1899	crystalline cellulose content	1871:1899	crystalline cellulose content	1871:1899	In addition, glcat14a-1glcat14c-1 mutants showed defects in pectin formation, calcium content and the degree of pectin methyl-esterification (DM) as well as reductions in crystalline cellulose content and seed size.					
34051740	8	107	theme	total	1616:1620	arg1	content					1631:1637	total mucilage content	1616:1637	total mucilage content	1616:1637	Also, a reduction in total mucilage content was observed in glcat14a-1glcat14c-1 mutants relative to WT.					
34051740	4	108	theme	AGPs	768:771	arg1	AGPs					768:771	AGPs	768:771	AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes	768:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	4	108	theme	AGPs	768:771	arg1	residues					756:763	the glucuronic acid residues	736:763	the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes	736:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	4	108	theme	AGPs	768:771	arg1	residue					725:731	One such sugar residue	710:731	One such sugar residue	710:731	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	11	109	theme	redundant	2134:2142	arg1	roles					2144:2148	partially redundant roles	2124:2148	partially redundant roles	2124:2148	We propose that the enzymatic activities of GLCAT14A and GLCAT14C play partially redundant roles and are required for the organization of the mucilage matrix and seed size in Arabidopsis thaliana.					
34051740	10	110	theme	important	1948:1956	arg1	questions					1958:1966	important questions	1948:1966	important questions regarding cell wall polymer interactions and organization during mucilage formation	1948:2050	CONCLUSIONS These results raise important questions regarding cell wall polymer interactions and organization during mucilage formation.					
34051740	2	111	theme	functions	459:467	arg1	establishment					426:438	the establishment	422:438	the establishment of normal cellular functions	422:467	Type II AGs are necessary for plant growth and critically important for the establishment of normal cellular functions.					
34051740	8	112	from	a reduction	1601:1611	arg1	content					1631:1637	total mucilage content	1616:1637	total mucilage content	1616:1637	Also, a reduction in total mucilage content was observed in glcat14a-1glcat14c-1 mutants relative to WT.					
34051740	4	113	theme	genes/enzymes	854:866	arg1	action					818:823	the action	814:823	the action of β-glucuronosyltransferase genes/enzymes	814:866	One such sugar residue is the glucuronic acid residues of AGPs that are transferred onto AGP glycans by the action of β-glucuronosyltransferase genes/enzymes.					
34051740	2	114	theme	normal	443:448	arg1	functions					459:467	normal cellular functions	443:467	normal cellular functions	443:467	Type II AGs are necessary for plant growth and critically important for the establishment of normal cellular functions.					
34051740	0	115	theme	type	63:66	arg1	arabinogalactans					71:86	type II arabinogalactans	63:86	type II arabinogalactans	63:86	Two β-glucuronosyltransferases involved in the biosynthesis of type II arabinogalactans function in mucilage polysaccharide matrix organization in Arabidopsis thaliana.					
34051740	8	116	theme	glcat14a-1glcat14c-1	1655:1674	arg1	mutants					1676:1682	glcat14a-1glcat14c-1 mutants	1655:1682	glcat14a-1glcat14c-1 mutants relative to WT	1655:1697	Also, a reduction in total mucilage content was observed in glcat14a-1glcat14c-1 mutants relative to WT.					
34051740	10	117	theme	wall	1983:1986	arg1	interactions					1996:2007	cell wall polymer interactions	1978:2007	cell wall polymer interactions	1978:2007	CONCLUSIONS These results raise important questions regarding cell wall polymer interactions and organization during mucilage formation.					
34051740	6	118	theme	pectin	1155:1160	arg1	HG					1180:1181	HG	1180:1181	HG	1180:1181	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	6	118	theme	pectin	1155:1160	arg1	homogalacturonan					1162:1177	mucilage pectin homogalacturonan	1146:1177	mucilage pectin homogalacturonan (HG)	1146:1182	Using a reverse genetics approach, we observed that glcat14a-1 mutants displayed subtle alterations in mucilage pectin homogalacturonan (HG) compared to wild type (WT), while glcat14a-1glcat14c-1 mutants displayed much more severe mucilage phenotypes, including loss of adherent mucilage and significant alterations in cellulose ray formation and seed coat morphology.					
34051740	8	119	theme	relative	1684:1691	arg1	mutants					1676:1682	glcat14a-1glcat14c-1 mutants	1655:1682	glcat14a-1glcat14c-1 mutants relative to WT	1655:1697	Also, a reduction in total mucilage content was observed in glcat14a-1glcat14c-1 mutants relative to WT.					
34051740	7	120	theme	non-adherent	1567:1578	arg1	mucilage					1585:1592	the adherent and non-adherent seed mucilage	1550:1592	the adherent and non-adherent seed mucilage	1550:1592	Monosaccharide composition analysis showed significant alterations in the sugar amounts of glcat14a-1glcat14c-1 mutants relative to WT in the adherent and non-adherent seed mucilage.					
34051740	8	121	located	observed	1643:1650	arg2	a reduction					1601:1611	a reduction	1601:1611	a reduction in total mucilage content	1601:1637	Also, a reduction in total mucilage content was observed in glcat14a-1glcat14c-1 mutants relative to WT.					
34051740	8	121	located	observed	1643:1650	arg1	mutants					1676:1682	glcat14a-1glcat14c-1 mutants	1655:1682	glcat14a-1glcat14c-1 mutants relative to WT	1655:1697	Also, a reduction in total mucilage content was observed in glcat14a-1glcat14c-1 mutants relative to WT.					
34051740	3	122	from	importance	482:491	arg1	development					517:527	plant development	511:527	plant development	511:527	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
34051740	3	123	theme	underlying	555:564	arg1	role					566:569	the underlying role	551:569	the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development	551:664	Despite the importance of type II AGs in plant development, our understanding of the underlying role of these glycans/sugar residues in mucilage formation and seed coat epidermal cell development is poorly understood and far from complete.					
32439449	6	0	theme	valeric	1137:1143	arg1	acid					1145:1148	valeric acid	1137:1148	valeric acid	1137:1148	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	2	1	theme	S1	321:322	arg1	compositions					305:316	monosaccharide compositions	290:316	monosaccharide compositions	290:316	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	2	1	theme	S1	321:322	arg1	homogeneity					230:240	homogeneity	230:240	homogeneity	230:240	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	2	1	theme	S1	321:322	arg1	weights					253:259	molecular weights	243:259	molecular weights	243:259	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	2	1	theme	S1	321:322	arg1	contents					277:284	major chemical contents	262:284	major chemical contents	262:284	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	6	2	theme	acid	1131:1134	arg1	levels					1113:1118	higher levels	1106:1118	higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid	1106:1200	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	2	theme	acid	1131:1134	arg1	abundance					1078:1086	higher abundance	1071:1086	higher abundance of Megaphaera	1071:1100	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	2	3	theme	molecular	243:251	arg1	weights					253:259	molecular weights	243:259	molecular weights	243:259	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	7	4	theme	prebiotic	1261:1269	arg1	functions					1271:1279	potential prebiotic functions	1251:1279	potential prebiotic functions	1251:1279	We concluded that S1 and S2 may have potential prebiotic functions.					
32439449	6	5	theme	acid	1038:1041	arg1	abundance					962:970	higher abundance	955:970	higher abundance of genera Butyricimonas and Megamonas	955:1008	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	5	theme	acid	1038:1041	arg1	levels					1021:1026	higher levels	1014:1026	higher levels of lactic acid	1014:1041	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	6	theme	higher	1106:1111	arg1	levels					1113:1118	higher levels	1106:1118	higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid	1106:1200	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	2	7	theme	chemical	268:275	arg1	contents					277:284	major chemical contents	262:284	major chemical contents	262:284	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	4	8	from	microbiota	575:584	arg1	vitro					589:593	vitro	589:593	vitro	589:593	Results showed that S1 and S2 have different impacts on human gut microbiota in vitro.					
32439449	6	9	theme	isovaleric	1168:1177	arg1	acid					1179:1182	isovaleric acid	1168:1182	isovaleric acid	1168:1182	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	4	10	contain	have	539:542	arg1	S2					536:537	S2	536:537	S2	536:537	Results showed that S1 and S2 have different impacts on human gut microbiota in vitro.					
32439449	4	10	contain	have	539:542	arg1	S1					529:530	S1	529:530	S1	529:530	Results showed that S1 and S2 have different impacts on human gut microbiota in vitro.					
32439449	4	10	contain	have	539:542	arg2	impacts					554:560	different impacts	544:560	different impacts	544:560	Results showed that S1 and S2 have different impacts on human gut microbiota in vitro.					
32439449	1	11	theme	Solanum	179:185	arg1	L					194:194	Solanum nigrum L	179:194	Solanum nigrum L	179:194	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	6	12	theme	acid	1162:1165	arg1	levels					1113:1118	higher levels	1106:1118	higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid	1106:1200	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	12	theme	acid	1162:1165	arg1	abundance					1078:1086	higher abundance	1071:1086	higher abundance of Megaphaera	1071:1100	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	5	13	theme	acid	930:933	arg1	abundance					783:791	the abundance	779:791	the abundance of 8 genera	779:803	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	13	theme	acid	930:933	arg1	production					813:822	the production	809:822	the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid	809:933	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	14	theme	acetic	827:832	arg1	acid					834:837	acetic acid	827:837	acetic acid	827:837	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	6	15	theme	higher	1071:1076	arg1	abundance					1078:1086	higher abundance	1071:1086	higher abundance of Megaphaera	1071:1100	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	1	16	from	L	194:194	arg1	S1					163:164	S1	163:164	S1	163:164	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	1	16	from	L	194:194	arg1	polysaccharides					146:160	two novel polysaccharides	136:160	two novel polysaccharides (S1 and S2) from Solanum nigrum L	136:194	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	1	16	from	L	194:194	arg1	S2					170:171	S2	170:171	S2	170:171	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	5	17	theme	acid	692:695	arg1	abundance					624:632	the abundance	620:632	the abundance of 9 genera	620:644	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	17	theme	acid	692:695	arg1	production					654:663	the production	650:663	the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid	650:746	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	18	theme	isobutyric	698:707	arg1	acid					709:712	isobutyric acid	698:712	isobutyric acid	698:712	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	1	19	theme	novel	140:144	arg1	S1					163:164	S1	163:164	S1	163:164	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	1	19	theme	novel	140:144	arg1	polysaccharides					146:160	two novel polysaccharides	136:160	two novel polysaccharides (S1 and S2) from Solanum nigrum L	136:194	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	1	19	theme	novel	140:144	arg1	S2					170:171	S2	170:171	S2	170:171	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	5	20	theme	propionic	668:676	arg1	acid					678:681	propionic acid	668:681	propionic acid	668:681	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	0	21	from	L.	46:47	arg1	polysaccharides					10:24	Two novel polysaccharides	0:24	Two novel polysaccharides from Solanum nigrum L.	0:47	Two novel polysaccharides from Solanum nigrum L. exert potential prebiotic effects in an in vitro fermentation model.					
32439449	6	22	theme	acid	1197:1200	arg1	levels					1113:1118	higher levels	1106:1118	higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid	1106:1200	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	22	theme	acid	1197:1200	arg1	abundance					1078:1086	higher abundance	1071:1086	higher abundance of Megaphaera	1071:1100	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	5	23	theme	acid	881:884	arg1	abundance					783:791	the abundance	779:791	the abundance of 8 genera	779:803	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	23	theme	acid	881:884	arg1	production					813:822	the production	809:822	the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid	809:933	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	0	24	theme	prebiotic	65:73	arg1	effects					75:81	potential prebiotic effects	55:81	potential prebiotic effects	55:81	Two novel polysaccharides from Solanum nigrum L. exert potential prebiotic effects in an in vitro fermentation model.					
32439449	5	25	theme	isovaleric	732:741	arg1	acid					743:746	isovaleric acid	732:746	isovaleric acid	732:746	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	26	theme	acid	850:853	arg1	abundance					783:791	the abundance	779:791	the abundance of 8 genera	779:803	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	26	theme	acid	850:853	arg1	production					813:822	the production	809:822	the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid	809:933	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	6	27	theme	S1	942:943	arg1	group					945:949	S1 group	942:949	S1 group	942:949	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	3	28	theme	community	405:413	arg1	production					442:451	human faecal microbial community and short-chain fatty acid production	382:451	human faecal microbial community and short-chain fatty acid production	382:451	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	5	29	theme	butyric	856:862	arg1	acid					864:867	butyric acid	856:867	butyric acid	856:867	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	3	30	theme	faecal	388:393	arg1	community					405:413	faecal microbial community	388:413	faecal microbial community	388:413	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	5	31	theme	acid	912:915	arg1	abundance					783:791	the abundance	779:791	the abundance of 8 genera	779:803	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	31	theme	acid	912:915	arg1	production					813:822	the production	809:822	the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid	809:933	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	0	32	theme	potential	55:63	arg1	effects					75:81	potential prebiotic effects	55:81	potential prebiotic effects	55:81	Two novel polysaccharides from Solanum nigrum L. exert potential prebiotic effects in an in vitro fermentation model.					
32439449	1	33	dep	polysaccharides	146:160	arg1	S1					163:164	S1	163:164	S1	163:164	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	1	33	dep	polysaccharides	146:160	arg1	polysaccharides					146:160	two novel polysaccharides	136:160	two novel polysaccharides (S1 and S2) from Solanum nigrum L	136:194	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	1	33	dep	polysaccharides	146:160	arg1	S2					170:171	S2	170:171	S2	170:171	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	3	34	theme	acid	437:440	arg1	production					442:451	human faecal microbial community and short-chain fatty acid production	382:451	human faecal microbial community and short-chain fatty acid production	382:451	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	5	35	theme	acid	723:726	arg1	abundance					624:632	the abundance	620:632	the abundance of 9 genera	620:644	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	35	theme	acid	723:726	arg1	production					654:663	the production	650:663	the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid	650:746	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	36	theme	succinic	921:928	arg1	acid					930:933	succinic acid	921:933	succinic acid	921:933	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	2	37	theme	monosaccharide	290:303	arg1	compositions					305:316	monosaccharide compositions	290:316	monosaccharide compositions	290:316	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	1	38	theme	nigrum	187:192	arg1	L					194:194	Solanum nigrum L	179:194	Solanum nigrum L	179:194	In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified.					
32439449	5	39	theme	valeric	887:893	arg1	acid					895:898	valeric acid	887:898	valeric acid	887:898	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	4	40	theme	different	544:552	arg1	impacts					554:560	different impacts	544:560	different impacts	544:560	Results showed that S1 and S2 have different impacts on human gut microbiota in vitro.					
32439449	6	41	theme	genera	975:980	arg1	Butyricimonas					982:994	genera Butyricimonas	975:994	genera Butyricimonas	975:994	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	42	theme	acid	1145:1148	arg1	levels					1113:1118	higher levels	1106:1118	higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid	1106:1200	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	42	theme	acid	1145:1148	arg1	abundance					1078:1086	higher abundance	1071:1086	higher abundance of Megaphaera	1071:1100	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	3	43	theme	fermentation	489:500	arg1	model					502:506	an in vitro fermentation model	477:506	an in vitro fermentation model	477:506	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	2	44	gly	homogeneity	230:240	arg1	S2					328:329	S2	328:329	S2	328:329	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	2	44	gly	homogeneity	230:240	arg1	S1					321:322	S1	321:322	S1	321:322	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	6	45	theme	butyric	1123:1129	arg1	acid					1131:1134	butyric acid	1123:1134	butyric acid	1123:1134	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	3	46	theme	in	480:481	arg1	model					502:506	an in vitro fermentation model	477:506	an in vitro fermentation model	477:506	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	6	47	theme	lactic	1031:1036	arg1	acid					1038:1041	lactic acid	1031:1041	lactic acid	1031:1041	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	48	theme	succinic	1188:1195	arg1	acid					1197:1200	succinic acid	1188:1200	succinic acid	1188:1200	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	2	49	theme	major	262:266	arg1	contents					277:284	major chemical contents	262:284	major chemical contents	262:284	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	7	50	theme	potential	1251:1259	arg1	functions					1271:1279	potential prebiotic functions	1251:1279	potential prebiotic functions	1251:1279	We concluded that S1 and S2 may have potential prebiotic functions.					
32439449	4	51	theme	gut	571:573	arg1	microbiota					575:584	human gut microbiota	565:584	human gut microbiota in vitro	565:593	Results showed that S1 and S2 have different impacts on human gut microbiota in vitro.					
32439449	6	52	theme	acid	1179:1182	arg1	levels					1113:1118	higher levels	1106:1118	higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid	1106:1200	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	52	theme	acid	1179:1182	arg1	abundance					1078:1086	higher abundance	1071:1086	higher abundance of Megaphaera	1071:1100	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	53	theme	Megaphaera	1091:1100	arg1	levels					1113:1118	higher levels	1106:1118	higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid	1106:1200	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	53	theme	Megaphaera	1091:1100	arg1	abundance					1078:1086	higher abundance	1071:1086	higher abundance of Megaphaera	1071:1100	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	3	54	dep	in	480:481	arg1	vitro					483:487	vitro	483:487	vitro	483:487	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	5	55	theme	genera	639:644	arg1	abundance					624:632	the abundance	620:632	the abundance of 9 genera	620:644	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	55	theme	genera	639:644	arg1	production					654:663	the production	650:663	the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid	650:746	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	6	56	theme	isobutyric	1151:1160	arg1	acid					1162:1165	isobutyric acid	1151:1165	isobutyric acid	1151:1165	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	5	57	theme	acid	834:837	arg1	abundance					783:791	the abundance	779:791	the abundance of 8 genera	779:803	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	57	theme	acid	834:837	arg1	production					813:822	the production	809:822	the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid	809:933	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	3	58	theme	short-chain	419:429	arg1	acid					437:440	short-chain fatty acid	419:440	short-chain fatty acid	419:440	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	5	59	theme	butyric	684:690	arg1	acid					692:695	butyric acid	684:695	butyric acid	684:695	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	60	theme	genera	798:803	arg1	abundance					783:791	the abundance	779:791	the abundance of 8 genera	779:803	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	60	theme	genera	798:803	arg1	production					813:822	the production	809:822	the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid	809:933	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	6	61	theme	S2	1058:1059	arg1	group					1061:1065	S2 group	1058:1065	S2 group	1058:1065	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	3	62	from	effects	358:364	arg1	production					442:451	human faecal microbial community and short-chain fatty acid production	382:451	human faecal microbial community and short-chain fatty acid production	382:451	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	6	63	contain	had	951:953	arg1	group					945:949	S1 group	942:949	S1 group	942:949	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	63	contain	had	951:953	arg2	levels					1021:1026	higher levels	1014:1026	higher levels of lactic acid	1014:1041	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	63	contain	had	951:953	arg2	abundance					962:970	higher abundance	955:970	higher abundance of genera Butyricimonas and Megamonas	955:1008	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	0	64	theme	in	89:90	arg1	model					111:115	an in vitro fermentation model	86:115	an in vitro fermentation model	86:115	Two novel polysaccharides from Solanum nigrum L. exert potential prebiotic effects in an in vitro fermentation model.					
32439449	5	65	theme	acid	864:867	arg1	abundance					783:791	the abundance	779:791	the abundance of 8 genera	779:803	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	65	theme	acid	864:867	arg1	production					813:822	the production	809:822	the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid	809:933	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	0	66	theme	fermentation	98:109	arg1	model					111:115	an in vitro fermentation model	86:115	an in vitro fermentation model	86:115	Two novel polysaccharides from Solanum nigrum L. exert potential prebiotic effects in an in vitro fermentation model.					
32439449	0	67	dep	in	89:90	arg1	vitro					92:96	vitro	92:96	vitro	92:96	Two novel polysaccharides from Solanum nigrum L. exert potential prebiotic effects in an in vitro fermentation model.					
32439449	6	68	theme	higher	955:960	arg1	abundance					962:970	higher abundance	955:970	higher abundance of genera Butyricimonas and Megamonas	955:1008	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	3	69	theme	human	382:386	arg1	production					442:451	human faecal microbial community and short-chain fatty acid production	382:451	human faecal microbial community and short-chain fatty acid production	382:451	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	0	70	theme	novel	4:8	arg1	polysaccharides					10:24	Two novel polysaccharides	0:24	Two novel polysaccharides from Solanum nigrum L.	0:47	Two novel polysaccharides from Solanum nigrum L. exert potential prebiotic effects in an in vitro fermentation model.					
32439449	5	71	theme	propionic	840:848	arg1	acid					850:853	propionic acid	840:853	propionic acid	840:853	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	3	72	theme	S2	376:377	arg1	effects					358:364	the effects	354:364	the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production	354:451	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	5	73	theme	acid	743:746	arg1	abundance					624:632	the abundance	620:632	the abundance of 9 genera	620:644	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	73	theme	acid	743:746	arg1	production					654:663	the production	650:663	the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid	650:746	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	74	theme	isobutyric	870:879	arg1	acid					881:884	isobutyric acid	870:884	isobutyric acid	870:884	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	75	theme	acid	709:712	arg1	abundance					624:632	the abundance	620:632	the abundance of 9 genera	620:644	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	75	theme	acid	709:712	arg1	production					654:663	the production	650:663	the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid	650:746	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	7	76	contain	have	1246:1249	arg2	functions					1271:1279	potential prebiotic functions	1251:1279	potential prebiotic functions	1251:1279	We concluded that S1 and S2 may have potential prebiotic functions.					
32439449	7	76	contain	have	1246:1249	arg1	S1					1232:1233	S1	1232:1233	S1	1232:1233	We concluded that S1 and S2 may have potential prebiotic functions.					
32439449	7	76	contain	have	1246:1249	arg1	S2					1239:1240	S2	1239:1240	S2	1239:1240	We concluded that S1 and S2 may have potential prebiotic functions.					
32439449	5	77	theme	isovaleric	901:910	arg1	acid					912:915	isovaleric acid	901:915	isovaleric acid	901:915	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	3	78	theme	S1	369:370	arg1	effects					358:364	the effects	354:364	the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production	354:451	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	3	79	theme	microbial	395:403	arg1	community					405:413	faecal microbial community	388:413	faecal microbial community	388:413	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	5	80	theme	valeric	715:721	arg1	acid					723:726	valeric acid	715:726	valeric acid	715:726	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	6	81	theme	higher	1014:1019	arg1	levels					1021:1026	higher levels	1014:1026	higher levels of lactic acid	1014:1041	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	82	contain	had	1067:1069	arg1	group					1061:1065	S2 group	1058:1065	S2 group	1058:1065	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	82	contain	had	1067:1069	arg2	levels					1113:1118	higher levels	1106:1118	higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid	1106:1200	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	82	contain	had	1067:1069	arg2	abundance					1078:1086	higher abundance	1071:1086	higher abundance of Megaphaera	1071:1100	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	83	theme	Megamonas	1000:1008	arg1	abundance					962:970	higher abundance	955:970	higher abundance of genera Butyricimonas and Megamonas	955:1008	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	83	theme	Megamonas	1000:1008	arg1	levels					1021:1026	higher levels	1014:1026	higher levels of lactic acid	1014:1041	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	5	84	theme	acid	678:681	arg1	abundance					624:632	the abundance	620:632	the abundance of 9 genera	620:644	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	84	theme	acid	678:681	arg1	production					654:663	the production	650:663	the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid	650:746	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	3	85	theme	fatty	431:435	arg1	acid					437:440	short-chain fatty acid	419:440	short-chain fatty acid	419:440	Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model.					
32439449	5	86	theme	acid	895:898	arg1	abundance					783:791	the abundance	779:791	the abundance of 8 genera	779:803	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	5	86	theme	acid	895:898	arg1	production					813:822	the production	809:822	the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid	809:933	S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid.					
32439449	6	87	theme	Butyricimonas	982:994	arg1	abundance					962:970	higher abundance	955:970	higher abundance of genera Butyricimonas and Megamonas	955:1008	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	6	87	theme	Butyricimonas	982:994	arg1	levels					1021:1026	higher levels	1014:1026	higher levels of lactic acid	1014:1041	Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably.					
32439449	4	88	theme	human	565:569	arg1	microbiota					575:584	human gut microbiota	565:584	human gut microbiota in vitro	565:593	Results showed that S1 and S2 have different impacts on human gut microbiota in vitro.					
32439449	2	89	theme	S2	328:329	arg1	compositions					305:316	monosaccharide compositions	290:316	monosaccharide compositions	290:316	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	2	89	theme	S2	328:329	arg1	homogeneity					230:240	homogeneity	230:240	homogeneity	230:240	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	2	89	theme	S2	328:329	arg1	weights					253:259	molecular weights	243:259	molecular weights	243:259	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32439449	2	89	theme	S2	328:329	arg1	contents					277:284	major chemical contents	262:284	major chemical contents	262:284	Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined.					
32498075	7	0	theme	electrical	1180:1189	arg1	conductivity					1191:1202	the electrical conductivity	1176:1202	the electrical conductivity	1176:1202	These results have confirmed that the electrical conductivity and flexibility were influenced by the type of cellulose, and MC-PANI was found to have the best performance in the electrical conductivity and flexibility.					
32498075	0	1	with	fabrics	42:48	arg1	flexibility					83:93	flexibility	83:93	flexibility	83:93	Development of cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	0	1	with	fabrics	42:48	arg1	conductivity					66:77	electrical conductivity	55:77	electrical conductivity	55:77	Development of cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	3	2	theme	time-of-flight	513:526	arg1	analysis					546:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis	469:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis	469:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	2	3	with	polymerized	373:383	arg1	aniline					390:396	aniline	390:396	aniline	390:396	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	3	4	theme	mass	528:531	arg1	analysis					546:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis	469:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis	469:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	4	5	theme	BC-PANI	830:836	arg1	conductivity					814:825	The electrical conductivity	799:825	The electrical conductivity of BC-PANI and CC-PANI	799:848	The electrical conductivity of BC-PANI and CC-PANI were ranging from 0.962 × 10-2 S/cm to 2.840 × 10-2 S/cm.					
32498075	6	6	contain	had	1068:1070	arg1	MC-PANI					1060:1066	MC-PANI	1060:1066	MC-PANI	1060:1066	The flexibility and crease recovery results showed that MC-PANI had the highest flexibility compared to BC-PANI, HPMC-PANI, and CMC-PANI.					
32498075	6	6	contain	had	1068:1070	arg2	flexibility					1084:1094	the highest flexibility	1072:1094	the highest flexibility	1072:1094	The flexibility and crease recovery results showed that MC-PANI had the highest flexibility compared to BC-PANI, HPMC-PANI, and CMC-PANI.					
32498075	7	7	theme	best	1296:1299	arg1	performance					1301:1311	the best performance	1292:1311	the best performance	1292:1311	These results have confirmed that the electrical conductivity and flexibility were influenced by the type of cellulose, and MC-PANI was found to have the best performance in the electrical conductivity and flexibility.					
32498075	3	8	theme	CC-PANI	576:582	arg1	composites					584:593	three CC-PANI composites	570:593	three CC-PANI composites	570:593	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	2	9	dep	cellulose	215:223	arg1	MC					290:291	MC	290:291	MC	290:291	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	2	9	dep	cellulose	215:223	arg1	cellulose					279:287	methyl cellulose	272:287	methyl cellulose (MC)	272:292	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	7	10	theme	cellulose	1251:1259	arg1	type					1243:1246	the type	1239:1246	the type of cellulose	1239:1259	These results have confirmed that the electrical conductivity and flexibility were influenced by the type of cellulose, and MC-PANI was found to have the best performance in the electrical conductivity and flexibility.					
32498075	2	11	theme	carboxymethyl	330:342	arg1	CMC					355:357	CMC	355:357	CMC	355:357	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	2	11	theme	carboxymethyl	330:342	arg1	cellulose					344:352	carboxymethyl cellulose	330:352	carboxymethyl cellulose (CMC)	330:358	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	1	12	with	fabrics	151:157	arg1	conductivity					175:186	electrical conductivity	164:186	electrical conductivity	164:186	This study aimed to produce cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	1	12	with	fabrics	151:157	arg1	flexibility					192:202	flexibility	192:202	flexibility	192:202	This study aimed to produce cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	4	13	theme	CC-PANI	842:848	arg1	conductivity					814:825	The electrical conductivity	799:825	The electrical conductivity of BC-PANI and CC-PANI	799:848	The electrical conductivity of BC-PANI and CC-PANI were ranging from 0.962 × 10-2 S/cm to 2.840 × 10-2 S/cm.					
32498075	4	14	theme	2.840	889:893	arg1	×					895:895	×	895:895	×	895:895	The electrical conductivity of BC-PANI and CC-PANI were ranging from 0.962 × 10-2 S/cm to 2.840 × 10-2 S/cm.					
32498075	3	15	theme	laser	485:489	arg1	desorption/ionization					491:511	Matrix-assisted laser desorption/ionization	469:511	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis	469:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	3	16	theme	longer	605:610	arg1	pattern					643:649	longer and more stable polymerization pattern	605:649	longer and more stable polymerization pattern than BC-PANI	605:662	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	7	17	theme	electrical	1320:1329	arg1	conductivity					1331:1342	the electrical conductivity	1316:1342	the electrical conductivity	1316:1342	These results have confirmed that the electrical conductivity and flexibility were influenced by the type of cellulose, and MC-PANI was found to have the best performance in the electrical conductivity and flexibility.					
32498075	2	18	theme	cellulose	422:430	arg1	fabrics					432:438	the four conductive cellulose fabrics	402:438	the four conductive cellulose fabrics	402:438	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	4	19	theme	10-2	897:900	arg1	S/cm					902:905	2.840 × 10-2 S/cm	889:905	2.840 × 10-2 S/cm	889:905	The electrical conductivity of BC-PANI and CC-PANI were ranging from 0.962 × 10-2 S/cm to 2.840 × 10-2 S/cm.					
32498075	4	20	theme	×	895:895	arg1	S/cm					902:905	2.840 × 10-2 S/cm	889:905	2.840 × 10-2 S/cm	889:905	The electrical conductivity of BC-PANI and CC-PANI were ranging from 0.962 × 10-2 S/cm to 2.840 × 10-2 S/cm.					
32498075	2	21	theme	conductive	411:420	arg1	fabrics					432:438	the four conductive cellulose fabrics	402:438	the four conductive cellulose fabrics	402:438	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	6	22	theme	recovery	1031:1038	arg1	results					1040:1046	The flexibility and crease recovery results	1004:1046	The flexibility and crease recovery results	1004:1046	The flexibility and crease recovery results showed that MC-PANI had the highest flexibility compared to BC-PANI, HPMC-PANI, and CMC-PANI.					
32498075	3	23	theme	stable	621:626	arg1	pattern					643:649	longer and more stable polymerization pattern	605:649	longer and more stable polymerization pattern than BC-PANI	605:662	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	2	24	theme	hydroxypropyl	295:307	arg1	HPMC					320:323	HPMC	320:323	HPMC	320:323	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	2	24	theme	hydroxypropyl	295:307	arg1	cellulose					309:317	hydroxypropyl cellulose	295:317	hydroxypropyl cellulose (HPMC)	295:324	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	4	25	theme	10-2	876:879	arg1	S/cm					881:884	0.962 × 10-2 S/cm	868:884	0.962 × 10-2 S/cm	868:884	The electrical conductivity of BC-PANI and CC-PANI were ranging from 0.962 × 10-2 S/cm to 2.840 × 10-2 S/cm.					
32498075	0	26	theme	cellulose-based	15:29	arg1	fabrics					42:48	cellulose-based conductive fabrics	15:48	cellulose-based conductive fabrics with electrical conductivity and flexibility	15:93	Development of cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	6	27	theme	crease	1024:1029	arg1	recovery					1031:1038	crease recovery	1024:1038	crease recovery	1024:1038	The flexibility and crease recovery results showed that MC-PANI had the highest flexibility compared to BC-PANI, HPMC-PANI, and CMC-PANI.					
32498075	3	28	theme	polymerization	689:702	arg1	method					704:709	the different polymerization method	675:709	the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI	675:782	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	4	29	theme	electrical	803:812	arg1	conductivity					814:825	The electrical conductivity	799:825	The electrical conductivity of BC-PANI and CC-PANI	799:848	The electrical conductivity of BC-PANI and CC-PANI were ranging from 0.962 × 10-2 S/cm to 2.840 × 10-2 S/cm.					
32498075	3	30	theme	spectroscopy	533:544	arg1	analysis					546:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis	469:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis	469:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	3	31	theme	desorption/ionization	491:511	arg1	analysis					546:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis	469:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis	469:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	3	32	theme	polymerization	628:641	arg1	pattern					643:649	longer and more stable polymerization pattern	605:649	longer and more stable polymerization pattern than BC-PANI	605:662	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	0	33	theme	fabrics	42:48	arg1	Development					0:10	Development	0:10	Development of cellulose-based conductive fabrics with electrical conductivity and flexibility.	0:94	Development of cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	6	34	theme	flexibility	1008:1018	arg1	results					1040:1046	The flexibility and crease recovery results	1004:1046	The flexibility and crease recovery results	1004:1046	The flexibility and crease recovery results showed that MC-PANI had the highest flexibility compared to BC-PANI, HPMC-PANI, and CMC-PANI.					
32498075	2	35	theme	Bacterial	205:213	arg1	cellulose					215:223	Bacterial cellulose	205:223	Bacterial cellulose (BC)	205:228	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	2	35	theme	Bacterial	205:213	arg1	CC					260:261	CC	260:261	CC	260:261	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	2	35	theme	Bacterial	205:213	arg1	BC					226:227	BC	226:227	BC	226:227	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	0	36	theme	conductive	31:40	arg1	fabrics					42:48	cellulose-based conductive fabrics	15:48	cellulose-based conductive fabrics with electrical conductivity and flexibility	15:93	Development of cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	5	37	theme	highest	927:933	arg1	conductivity					946:957	the highest electrical conductivity	923:957	the highest electrical conductivity among the four conductive cellulose fabrics	923:1001	MC-PANI showed the highest electrical conductivity among the four conductive cellulose fabrics.					
32498075	3	38	theme	different	679:687	arg1	method					704:709	the different polymerization method	675:709	the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI	675:782	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	1	39	theme	cellulose-based	124:138	arg1	fabrics					151:157	cellulose-based conductive fabrics	124:157	cellulose-based conductive fabrics with electrical conductivity and flexibility	124:202	This study aimed to produce cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	0	40	theme	electrical	55:64	arg1	conductivity					66:77	electrical conductivity	55:77	electrical conductivity	55:77	Development of cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	2	41	theme	chemical	240:247	arg1	cellulose					249:257	three chemical cellulose	234:257	three chemical cellulose	234:257	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	1	42	theme	conductive	140:149	arg1	fabrics					151:157	cellulose-based conductive fabrics	124:157	cellulose-based conductive fabrics with electrical conductivity and flexibility	124:202	This study aimed to produce cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	2	43	theme	methyl	272:277	arg1	MC					290:291	MC	290:291	MC	290:291	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	2	43	theme	methyl	272:277	arg1	cellulose					279:287	methyl cellulose	272:287	methyl cellulose (MC)	272:292	Bacterial cellulose (BC) and three chemical cellulose (CC), namely methyl cellulose (MC), hydroxypropyl cellulose (HPMC) and carboxymethyl cellulose (CMC) were in situ polymerized with aniline and the four conductive cellulose fabrics were compared and evaluated.					
32498075	4	44	theme	×	874:874	arg1	S/cm					881:884	0.962 × 10-2 S/cm	868:884	0.962 × 10-2 S/cm	868:884	The electrical conductivity of BC-PANI and CC-PANI were ranging from 0.962 × 10-2 S/cm to 2.840 × 10-2 S/cm.					
32498075	5	45	theme	conductive	974:983	arg1	fabrics					995:1001	the four conductive cellulose fabrics	965:1001	the four conductive cellulose fabrics	965:1001	MC-PANI showed the highest electrical conductivity among the four conductive cellulose fabrics.					
32498075	3	46	theme	Matrix-assisted	469:483	arg1	desorption/ionization					491:511	Matrix-assisted laser desorption/ionization	469:511	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis	469:553	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	5	47	theme	cellulose	985:993	arg1	fabrics					995:1001	the four conductive cellulose fabrics	965:1001	the four conductive cellulose fabrics	965:1001	MC-PANI showed the highest electrical conductivity among the four conductive cellulose fabrics.					
32498075	3	48	theme	bulk	712:715	arg1	polymerization					717:730	bulk polymerization	712:730	the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI	675:782	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	4	49	theme	0.962	868:872	arg1	×					874:874	×	874:874	×	874:874	The electrical conductivity of BC-PANI and CC-PANI were ranging from 0.962 × 10-2 S/cm to 2.840 × 10-2 S/cm.					
32498075	1	50	theme	electrical	164:173	arg1	conductivity					175:186	electrical conductivity	164:186	electrical conductivity	164:186	This study aimed to produce cellulose-based conductive fabrics with electrical conductivity and flexibility.					
32498075	6	51	theme	highest	1076:1082	arg1	flexibility					1084:1094	the highest flexibility	1072:1094	the highest flexibility	1072:1094	The flexibility and crease recovery results showed that MC-PANI had the highest flexibility compared to BC-PANI, HPMC-PANI, and CMC-PANI.					
32498075	3	52	theme	emulsion	748:755	arg1	polymerization					757:770	emulsion polymerization	748:770	emulsion polymerization	748:770	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32498075	7	53	contain	have	1287:1290	arg1	MC-PANI					1266:1272	MC-PANI	1266:1272	MC-PANI	1266:1272	These results have confirmed that the electrical conductivity and flexibility were influenced by the type of cellulose, and MC-PANI was found to have the best performance in the electrical conductivity and flexibility.					
32498075	7	53	contain	have	1287:1290	arg2	performance					1301:1311	the best performance	1292:1311	the best performance	1292:1311	These results have confirmed that the electrical conductivity and flexibility were influenced by the type of cellulose, and MC-PANI was found to have the best performance in the electrical conductivity and flexibility.					
32498075	5	54	theme	electrical	935:944	arg1	conductivity					946:957	the highest electrical conductivity	923:957	the highest electrical conductivity among the four conductive cellulose fabrics	923:1001	MC-PANI showed the highest electrical conductivity among the four conductive cellulose fabrics.					
32498075	3	55	dep	method	704:709	arg1	polymerization					717:730	bulk polymerization	712:730	the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI	675:782	Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy analysis confirmed that three CC-PANI composites displayed longer and more stable polymerization pattern than BC-PANI because of the different polymerization method: bulk polymerization for BC-PANI and emulsion polymerization for CC-PANI, respectively.					
32134638	4	0	from	stability	706:714	arg1	vitro					719:723	vitro	719:723	vitro	719:723	ADCs generated via this approach demonstrate excellent stability in vitro as well as strong efficacy in vitro and in vivo.					
32134638	3	1	theme	IgG-type	630:637	arg1	antibodies					639:648	native, fully glycosylated IgG-type antibodies	603:648	native, fully glycosylated IgG-type antibodies	603:648	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	3	2	theme	drug-linker	557:567	arg1	constructs					569:578	drug-linker constructs	557:578	drug-linker constructs	557:578	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	5	3	theme	several	825:831	arg1	antibodies					840:849	several native antibodies	825:849	several native antibodies	825:849	As it employs different drug-linker structures and several native antibodies, our study additionally proves the broad applicability of this approach.					
32134638	2	4	theme	sequence	398:405	arg1	manipulation					369:380	undesired manipulation	359:380	undesired manipulation of the antibody sequence or its glycan structures	359:430	However, most of the technologies developed so far require undesired manipulation of the antibody sequence or its glycan structures.					
32134638	1	5	theme	Site-specific	93:105	arg1	technologies					122:133	Site-specific bioconjugation technologies	93:133	Site-specific bioconjugation technologies	93:133	Site-specific bioconjugation technologies are frequently employed to generate homogeneous antibody-drug conjugates (ADCs) and are generally considered superior to stochastic approaches like lysine coupling.					
32134638	3	6	theme	transglutaminase	491:506	arg1	engineering					466:476	the successful engineering	451:476	the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies	451:648	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	5	7	theme	broad	886:890	arg1	applicability					892:904	the broad applicability	882:904	the broad applicability of this approach	882:921	As it employs different drug-linker structures and several native antibodies, our study additionally proves the broad applicability of this approach.					
32134638	1	8	theme	bioconjugation	107:120	arg1	technologies					122:133	Site-specific bioconjugation technologies	93:133	Site-specific bioconjugation technologies	93:133	Site-specific bioconjugation technologies are frequently employed to generate homogeneous antibody-drug conjugates (ADCs) and are generally considered superior to stochastic approaches like lysine coupling.					
32134638	3	9	theme	native	603:608	arg1	antibodies					639:648	native, fully glycosylated IgG-type antibodies	603:648	native, fully glycosylated IgG-type antibodies	603:648	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	4	10	from	efficacy	743:750	arg1	vitro					719:723	vitro	719:723	vitro	719:723	ADCs generated via this approach demonstrate excellent stability in vitro as well as strong efficacy in vitro and in vivo.					
32134638	4	11	theme	strong	736:741	arg1	efficacy					743:750	strong efficacy	736:750	excellent stability in vitro as well as strong efficacy	696:750	ADCs generated via this approach demonstrate excellent stability in vitro as well as strong efficacy in vitro and in vivo.					
32134638	0	12	theme	Site-Specific	0:12	arg1	Conjugation					14:24	Site-Specific Conjugation	0:24	Site-Specific Conjugation of Native Antibodies	0:45	Site-Specific Conjugation of Native Antibodies Using Engineered Microbial Transglutaminases.					
32134638	2	13	theme	undesired	359:367	arg1	manipulation					369:380	undesired manipulation	359:380	undesired manipulation of the antibody sequence or its glycan structures	359:430	However, most of the technologies developed so far require undesired manipulation of the antibody sequence or its glycan structures.					
32134638	3	14	theme	microbial	481:489	arg1	transglutaminase					491:506	microbial transglutaminase	481:506	microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies	481:648	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	0	15	theme	Native	29:34	arg1	Antibodies					36:45	Native Antibodies	29:45	Native Antibodies	29:45	Site-Specific Conjugation of Native Antibodies Using Engineered Microbial Transglutaminases.					
32134638	3	16	theme	antibodies	639:648	arg1	position					583:590	position HC-Q295	583:598	position HC-Q295 of native, fully glycosylated IgG-type antibodies	583:648	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	5	17	theme	approach	914:921	arg1	applicability					892:904	the broad applicability	882:904	the broad applicability of this approach	882:921	As it employs different drug-linker structures and several native antibodies, our study additionally proves the broad applicability of this approach.					
32134638	3	18	dep	native	603:608	arg1	glycosylated					617:628	glycosylated	617:628	glycosylated	617:628	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	3	19	theme	successful	455:464	arg1	engineering					466:476	the successful engineering	451:476	the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies	451:648	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	5	20	theme	different	788:796	arg1	structures					810:819	different drug-linker structures	788:819	different drug-linker structures	788:819	As it employs different drug-linker structures and several native antibodies, our study additionally proves the broad applicability of this approach.					
32134638	1	21	theme	stochastic	256:265	arg1	approaches					267:276	stochastic approaches	256:276	stochastic approaches like lysine coupling	256:297	Site-specific bioconjugation technologies are frequently employed to generate homogeneous antibody-drug conjugates (ADCs) and are generally considered superior to stochastic approaches like lysine coupling.					
32134638	3	22	theme	efficient	517:525	arg1	conjugation					542:552	efficient, site-specific conjugation	517:552	efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies	517:648	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	0	23	theme	Antibodies	36:45	arg1	Conjugation					14:24	Site-Specific Conjugation	0:24	Site-Specific Conjugation of Native Antibodies	0:45	Site-Specific Conjugation of Native Antibodies Using Engineered Microbial Transglutaminases.					
32134638	5	24	theme	drug-linker	798:808	arg1	structures					810:819	different drug-linker structures	788:819	different drug-linker structures	788:819	As it employs different drug-linker structures and several native antibodies, our study additionally proves the broad applicability of this approach.					
32134638	5	25	theme	native	833:838	arg1	antibodies					840:849	several native antibodies	825:849	several native antibodies	825:849	As it employs different drug-linker structures and several native antibodies, our study additionally proves the broad applicability of this approach.					
32134638	0	26	theme	Microbial	64:72	arg1	Transglutaminases					74:90	Engineered Microbial Transglutaminases	53:90	Engineered Microbial Transglutaminases	53:90	Site-Specific Conjugation of Native Antibodies Using Engineered Microbial Transglutaminases.					
32134638	3	27	theme	constructs	569:578	arg1	conjugation					542:552	efficient, site-specific conjugation	517:552	efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies	517:648	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	0	28	theme	Engineered	53:62	arg1	Transglutaminases					74:90	Engineered Microbial Transglutaminases	53:90	Engineered Microbial Transglutaminases	53:90	Site-Specific Conjugation of Native Antibodies Using Engineered Microbial Transglutaminases.					
32134638	2	29	theme	antibody	389:396	arg1	sequence					398:405	the antibody sequence	385:405	the antibody sequence	385:405	However, most of the technologies developed so far require undesired manipulation of the antibody sequence or its glycan structures.					
32134638	3	30	dep	efficient	517:525	arg1	site-specific					528:540	site-specific	528:540	site-specific	528:540	Herein, we report the successful engineering of microbial transglutaminase enabling efficient, site-specific conjugation of drug-linker constructs to position HC-Q295 of native, fully glycosylated IgG-type antibodies.					
32134638	1	31	theme	homogeneous	171:181	arg1	ADCs					209:212	ADCs	209:212	ADCs	209:212	Site-specific bioconjugation technologies are frequently employed to generate homogeneous antibody-drug conjugates (ADCs) and are generally considered superior to stochastic approaches like lysine coupling.					
32134638	1	31	theme	homogeneous	171:181	arg1	conjugates					197:206	homogeneous antibody-drug conjugates	171:206	homogeneous antibody-drug conjugates (ADCs)	171:213	Site-specific bioconjugation technologies are frequently employed to generate homogeneous antibody-drug conjugates (ADCs) and are generally considered superior to stochastic approaches like lysine coupling.					
32134638	1	32	theme	antibody-drug	183:195	arg1	ADCs					209:212	ADCs	209:212	ADCs	209:212	Site-specific bioconjugation technologies are frequently employed to generate homogeneous antibody-drug conjugates (ADCs) and are generally considered superior to stochastic approaches like lysine coupling.					
32134638	1	32	theme	antibody-drug	183:195	arg1	conjugates					197:206	homogeneous antibody-drug conjugates	171:206	homogeneous antibody-drug conjugates (ADCs)	171:213	Site-specific bioconjugation technologies are frequently employed to generate homogeneous antibody-drug conjugates (ADCs) and are generally considered superior to stochastic approaches like lysine coupling.					
32134638	1	33	theme	lysine	283:288	arg1	coupling					290:297	lysine coupling	283:297	lysine coupling	283:297	Site-specific bioconjugation technologies are frequently employed to generate homogeneous antibody-drug conjugates (ADCs) and are generally considered superior to stochastic approaches like lysine coupling.					
32134638	2	34	theme	structures	421:430	arg1	manipulation					369:380	undesired manipulation	359:380	undesired manipulation of the antibody sequence or its glycan structures	359:430	However, most of the technologies developed so far require undesired manipulation of the antibody sequence or its glycan structures.					
32134638	4	35	theme	excellent	696:704	arg1	stability					706:714	excellent stability	696:714	excellent stability in vitro as well as strong efficacy	696:750	ADCs generated via this approach demonstrate excellent stability in vitro as well as strong efficacy in vitro and in vivo.					
32134638	2	36	theme	glycan	414:419	arg1	structures					421:430	its glycan structures	410:430	its glycan structures	410:430	However, most of the technologies developed so far require undesired manipulation of the antibody sequence or its glycan structures.					
32807542	6	0	theme	trapped	1016:1022	arg1	structure					1025:1033	the "trapped" structure	1011:1033	the "trapped" structure	1011:1033	In the "trapped" structure, the MP was the dominant structure of the composite gel network.					
32807542	9	1	theme	gel	1607:1609	arg1	properties					1611:1620	composite gel properties	1597:1620	composite gel properties	1597:1620	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	6	2	theme	network	1091:1097	arg1	structure					1060:1068	the dominant structure	1047:1068	the dominant structure of the composite gel network	1047:1097	In the "trapped" structure, the MP was the dominant structure of the composite gel network.					
32807542	6	2	theme	network	1091:1097	arg1	MP					1040:1041	the MP	1036:1041	the MP	1036:1041	In the "trapped" structure, the MP was the dominant structure of the composite gel network.					
32807542	3	3	theme	SEM	492:494	arg1	images					496:501	The SEM images	488:501	The SEM images	488:501	The SEM images indicated that moisture extrusion formed moisture channels and deteriorated the aggregation of MP gel networks during the thermal process.					
32807542	4	4	theme	gel	806:808	arg1	networks					810:817	compact and integral gel networks	785:817	compact and integral gel networks	785:817	The polysaccharides stabilized moisture and reduced the appearance of moisture channels in the gel network, thereby promoting the formation of compact and integral gel networks.					
32807542	6	5	theme	composite	1077:1085	arg1	network					1091:1097	the composite gel network	1073:1097	the composite gel network	1073:1097	In the "trapped" structure, the MP was the dominant structure of the composite gel network.					
32807542	5	6	theme	MP-polysaccharide	824:840	arg1	system					878:883	a thermally incompatible system	853:883	a thermally incompatible system	853:883	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	6	theme	MP-polysaccharide	824:840	arg1	mixture					842:848	The MP-polysaccharide mixture	820:848	The MP-polysaccharide mixture	820:848	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	7	dep	structure	958:966	arg1	1					941:941	1	941:941	1	941:941	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	3	8	theme	moisture	518:525	arg1	extrusion					527:535	moisture extrusion	518:535	moisture extrusion	518:535	The SEM images indicated that moisture extrusion formed moisture channels and deteriorated the aggregation of MP gel networks during the thermal process.					
32807542	8	9	dep	behaviour	1285:1293	arg1	the					1275:1277	the	1275:1277	the	1275:1277	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	6	10	theme	dominant	1051:1058	arg1	structure					1060:1068	the dominant structure	1047:1068	the dominant structure of the composite gel network	1047:1097	In the "trapped" structure, the MP was the dominant structure of the composite gel network.					
32807542	6	10	theme	dominant	1051:1058	arg1	MP					1040:1041	the MP	1036:1041	the MP	1036:1041	In the "trapped" structure, the MP was the dominant structure of the composite gel network.					
32807542	0	11	theme	moisture	109:116	arg1	stability					118:126	moisture stability	109:126	moisture stability	109:126	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	0	11	theme	moisture	109:116	arg1	effects					4:10	The effects	0:10	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.	0:127	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	2	12	theme	rheological	281:291	arg1	properties					293:302	rheological properties	281:302	rheological properties	281:302	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	1	13	theme	myofibrillar	215:226	arg1	MP					237:238	MP	237:238	MP	237:238	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels were studied.					
32807542	1	13	theme	myofibrillar	215:226	arg1	protein					228:234	myofibrillar protein	215:234	myofibrillar protein (MP) gels	215:244	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels were studied.					
32807542	8	14	theme	composite	1405:1413	arg1	gelation					1418:1425	composite MP gelation	1405:1425	composite MP gelation	1405:1425	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	8	15	dep	quality	1375:1381	arg1	the					1362:1364	the	1362:1364	the	1362:1364	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	8	16	theme	gelation	1418:1425	arg1	microstructure					1387:1400	microstructure	1387:1400	microstructure	1387:1400	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	8	16	theme	gelation	1418:1425	arg1	quality					1375:1381	textural quality	1366:1381	textural quality	1366:1381	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	5	17	theme	interpenetrated	980:994	arg1	forms					908:912	two main forms	899:912	two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure	899:1005	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	17	theme	interpenetrated	980:994	arg1	structure					997:1005	2) the "interpenetrated" structure	972:1005	2) the "interpenetrated" structure	972:1005	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	18	theme	main	903:906	arg1	forms					908:912	two main forms	899:912	two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure	899:1005	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	18	theme	main	903:906	arg1	structure					958:966	1) the "trapped" structure	941:966	1) the "trapped" structure	941:966	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	18	theme	main	903:906	arg1	structure					997:1005	2) the "interpenetrated" structure	972:1005	2) the "interpenetrated" structure	972:1005	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	2	19	theme	limited	456:462	arg1	effects					464:470	limited effects	456:470	limited effects	456:470	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	8	20	theme	Principal	1234:1242	arg1	analysis					1254:1261	Principal component analysis	1234:1261	Principal component analysis	1234:1261	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	5	21	dep	forms	908:912	arg1	forms					908:912	two main forms	899:912	two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure	899:1005	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	21	dep	forms	908:912	arg1	structure					958:966	1) the "trapped" structure	941:966	1) the "trapped" structure	941:966	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	21	dep	forms	908:912	arg1	structure					997:1005	2) the "interpenetrated" structure	972:1005	2) the "interpenetrated" structure	972:1005	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	0	22	theme	myofibrillar	67:78	arg1	protein					80:86	myofibrillar protein	67:86	myofibrillar protein	67:86	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	1	23	theme	polysaccharides	150:164	arg1	effects					133:139	The effects	129:139	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels	129:244	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels were studied.					
32807542	2	24	theme	%	476:476	arg1	addition					478:485	1% addition	475:485	1% addition	475:485	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	4	25	theme	moisture	712:719	arg1	channels					721:728	moisture channels	712:728	moisture channels	712:728	The polysaccharides stabilized moisture and reduced the appearance of moisture channels in the gel network, thereby promoting the formation of compact and integral gel networks.					
32807542	1	26	from	effects	133:139	arg1	microstructure					197:210	microstructure	197:210	microstructure	197:210	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels were studied.					
32807542	1	26	from	effects	133:139	arg1	properties					182:191	textural properties	173:191	textural properties	173:191	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels were studied.					
32807542	8	27	theme	phase	1279:1283	arg1	behaviour					1285:1293	phase behaviour	1279:1293	phase behaviour	1279:1293	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	9	28	theme	moisture	1488:1495	arg1	stability					1497:1505	moisture stability	1488:1505	moisture stability	1488:1505	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	9	29	theme	structure	1527:1535	arg1	"					1536:1536	a "trapped structure"	1516:1536	a "trapped structure" (phase behaviour)	1516:1554	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	9	29	theme	structure	1527:1535	arg1	behaviour					1545:1553	phase behaviour	1539:1553	phase behaviour	1539:1553	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	4	30	theme	channels	721:728	arg1	appearance					698:707	the appearance	694:707	the appearance of moisture channels in the gel network	694:747	The polysaccharides stabilized moisture and reduced the appearance of moisture channels in the gel network, thereby promoting the formation of compact and integral gel networks.					
32807542	5	31	theme	thermal	924:930	arg1	process					932:938	the thermal process	920:938	the thermal process	920:938	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	7	32	theme	networks	1224:1231	arg1	aggregation					1202:1212	the aggregation	1198:1212	the aggregation of MP gel networks	1198:1231	In the "interpenetrated" structure, the continuous polysaccharide hydrogel substantially hindered the aggregation of MP gel networks.					
32807542	3	33	theme	networks	605:612	arg1	aggregation					583:593	the aggregation	579:593	the aggregation of MP gel networks during the thermal process	579:639	The SEM images indicated that moisture extrusion formed moisture channels and deteriorated the aggregation of MP gel networks during the thermal process.					
32807542	8	34	theme	polysaccharides	1321:1335	arg1	behaviour					1285:1293	phase behaviour	1279:1293	phase behaviour	1279:1293	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	8	34	theme	polysaccharides	1321:1335	arg1	stability					1308:1316	moisture stability	1299:1316	moisture stability	1299:1316	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	6	35	from	structure	1060:1068	arg1	structure					1025:1033	the "trapped" structure	1011:1033	the "trapped" structure	1011:1033	In the "trapped" structure, the MP was the dominant structure of the composite gel network.					
32807542	2	36	theme	modified	391:398	arg1	MS					408:409	MS	408:409	MS	408:409	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	2	36	theme	modified	391:398	arg1	starch					400:405	modified starch	391:405	modified starch (MS)	391:410	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	0	37	theme	polysaccharides	21:35	arg1	behaviour					95:103	Phase behaviour	89:103	Phase behaviour	89:103	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	0	37	theme	polysaccharides	21:35	arg1	effects					4:10	The effects	0:10	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.	0:127	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	0	37	theme	polysaccharides	21:35	arg1	stability					118:126	moisture stability	109:126	moisture stability	109:126	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	7	38	theme	MP	1217:1218	arg1	networks					1224:1231	MP gel networks	1217:1231	MP gel networks	1217:1231	In the "interpenetrated" structure, the continuous polysaccharide hydrogel substantially hindered the aggregation of MP gel networks.					
32807542	2	39	theme	konjac	428:433	arg1	KG					448:449	KG	448:449	KG	448:449	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	2	39	theme	konjac	428:433	arg1	glucomannan					435:445	konjac glucomannan	428:445	konjac glucomannan (KG)	428:450	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	3	40	theme	MP	598:599	arg1	networks					605:612	MP gel networks	598:612	MP gel networks	598:612	The SEM images indicated that moisture extrusion formed moisture channels and deteriorated the aggregation of MP gel networks during the thermal process.					
32807542	8	41	theme	textural	1366:1373	arg1	quality					1375:1381	textural quality	1366:1381	textural quality	1366:1381	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	2	42	theme	gels	320:323	arg1	strength					268:275	gel strength	264:275	gel strength	264:275	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	2	42	theme	gels	320:323	arg1	properties					293:302	rheological properties	281:302	rheological properties	281:302	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	1	43	theme	protein	228:234	arg1	gels					241:244	myofibrillar protein (MP) gels	215:244	myofibrillar protein (MP) gels	215:244	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels were studied.					
32807542	9	44	theme	fat	1566:1568	arg1	polysaccharides					1453:1467	polysaccharides	1453:1467	polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour)	1453:1554	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	9	44	theme	fat	1566:1568	arg1	DF					1634:1635	DF	1634:1635	especially DF	1623:1635	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	9	44	theme	fat	1566:1568	arg1	replacements					1570:1581	ideal fat replacements	1560:1581	ideal fat replacements for improving composite gel properties	1560:1620	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	5	45	theme	"	956:956	arg1	forms					908:912	two main forms	899:912	two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure	899:1005	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	45	theme	"	956:956	arg1	structure					958:966	1) the "trapped" structure	941:966	1) the "trapped" structure	941:966	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	6	46	theme	"	1023:1023	arg1	structure					1025:1033	the "trapped" structure	1011:1033	the "trapped" structure	1011:1033	In the "trapped" structure, the MP was the dominant structure of the composite gel network.					
32807542	7	47	theme	interpenetrated	1108:1122	arg1	structure					1125:1133	the "interpenetrated" structure	1103:1133	the "interpenetrated" structure	1103:1133	In the "interpenetrated" structure, the continuous polysaccharide hydrogel substantially hindered the aggregation of MP gel networks.					
32807542	1	48	theme	gels	241:244	arg1	microstructure					197:210	microstructure	197:210	microstructure	197:210	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels were studied.					
32807542	1	48	theme	gels	241:244	arg1	properties					182:191	textural properties	173:191	textural properties	173:191	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels were studied.					
32807542	9	49	theme	composite	1597:1605	arg1	properties					1611:1620	composite gel properties	1597:1620	composite gel properties	1597:1620	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	7	50	theme	continuous	1140:1149	arg1	hydrogel					1166:1173	the continuous polysaccharide hydrogel	1136:1173	the continuous polysaccharide hydrogel	1136:1173	In the "interpenetrated" structure, the continuous polysaccharide hydrogel substantially hindered the aggregation of MP gel networks.					
32807542	6	51	theme	gel	1087:1089	arg1	network					1091:1097	the composite gel network	1073:1097	the composite gel network	1073:1097	In the "trapped" structure, the MP was the dominant structure of the composite gel network.					
32807542	2	52	theme	gel	264:266	arg1	strength					268:275	gel strength	264:275	gel strength	264:275	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	4	53	theme	integral	797:804	arg1	networks					810:817	compact and integral gel networks	785:817	compact and integral gel networks	785:817	The polysaccharides stabilized moisture and reduced the appearance of moisture channels in the gel network, thereby promoting the formation of compact and integral gel networks.					
32807542	7	54	theme	"	1123:1123	arg1	structure					1125:1133	the "interpenetrated" structure	1103:1133	the "interpenetrated" structure	1103:1133	In the "interpenetrated" structure, the continuous polysaccharide hydrogel substantially hindered the aggregation of MP gel networks.					
32807542	2	55	theme	composite	307:315	arg1	gels					320:323	composite MP gels	307:323	composite MP gels	307:323	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	1	56	theme	textural	173:180	arg1	properties					182:191	textural properties	173:191	textural properties	173:191	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels were studied.					
32807542	4	57	theme	networks	810:817	arg1	formation					772:780	the formation	768:780	the formation of compact and integral gel networks	768:817	The polysaccharides stabilized moisture and reduced the appearance of moisture channels in the gel network, thereby promoting the formation of compact and integral gel networks.					
32807542	8	58	theme	component	1244:1252	arg1	analysis					1254:1261	Principal component analysis	1234:1261	Principal component analysis	1234:1261	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	3	59	theme	moisture	544:551	arg1	channels					553:560	moisture channels	544:560	moisture channels	544:560	The SEM images indicated that moisture extrusion formed moisture channels and deteriorated the aggregation of MP gel networks during the thermal process.					
32807542	8	60	theme	MP	1415:1416	arg1	gelation					1418:1425	composite MP gelation	1405:1425	composite MP gelation	1405:1425	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	5	61	theme	"	995:995	arg1	forms					908:912	two main forms	899:912	two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure	899:1005	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	61	theme	"	995:995	arg1	structure					997:1005	2) the "interpenetrated" structure	972:1005	2) the "interpenetrated" structure	972:1005	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	0	62	theme	protein	80:86	arg1	properties					53:62	the gelation properties	40:62	the gelation properties of myofibrillar protein	40:86	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	5	63	theme	incompatible	865:876	arg1	system					878:883	a thermally incompatible system	853:883	a thermally incompatible system	853:883	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	63	theme	incompatible	865:876	arg1	mixture					842:848	The MP-polysaccharide mixture	820:848	The MP-polysaccharide mixture	820:848	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	0	64	from	effects	4:10	arg1	properties					53:62	the gelation properties	40:62	the gelation properties of myofibrillar protein	40:86	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	2	65	dep	strength	268:275	arg1	The					260:262	The	260:262	The	260:262	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	0	66	theme	Phase	89:93	arg1	behaviour					95:103	Phase behaviour	89:103	Phase behaviour	89:103	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	0	66	theme	Phase	89:93	arg1	effects					4:10	The effects	0:10	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.	0:127	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	2	67	theme	1	475:475	arg1	%					476:476	%	476:476	%	476:476	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	4	68	theme	gel	737:739	arg1	network					741:747	the gel network	733:747	the gel network	733:747	The polysaccharides stabilized moisture and reduced the appearance of moisture channels in the gel network, thereby promoting the formation of compact and integral gel networks.					
32807542	9	69	theme	trapped	1519:1525	arg1	"					1536:1536	a "trapped structure"	1516:1536	a "trapped structure" (phase behaviour)	1516:1554	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	9	69	theme	trapped	1519:1525	arg1	behaviour					1545:1553	phase behaviour	1539:1553	phase behaviour	1539:1553	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	0	70	dep	effects	4:10	arg1	behaviour					95:103	Phase behaviour	89:103	Phase behaviour	89:103	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	0	70	dep	effects	4:10	arg1	stability					118:126	moisture stability	109:126	moisture stability	109:126	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	0	70	dep	effects	4:10	arg1	effects					4:10	The effects	0:10	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.	0:127	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	4	71	from	appearance	698:707	arg1	network					741:747	the gel network	733:747	the gel network	733:747	The polysaccharides stabilized moisture and reduced the appearance of moisture channels in the gel network, thereby promoting the formation of compact and integral gel networks.					
32807542	7	72	theme	gel	1220:1222	arg1	networks					1224:1231	MP gel networks	1217:1231	MP gel networks	1217:1231	In the "interpenetrated" structure, the continuous polysaccharide hydrogel substantially hindered the aggregation of MP gel networks.					
32807542	3	73	theme	gel	601:603	arg1	networks					605:612	MP gel networks	598:612	MP gel networks	598:612	The SEM images indicated that moisture extrusion formed moisture channels and deteriorated the aggregation of MP gel networks during the thermal process.					
32807542	8	74	theme	moisture	1299:1306	arg1	stability					1308:1316	moisture stability	1299:1316	moisture stability	1299:1316	Principal component analysis showed that the phase behaviour and moisture stability of polysaccharides significantly influenced the textural quality and microstructure of composite MP gelation.					
32807542	9	75	theme	phase	1539:1543	arg1	"					1536:1536	a "trapped structure"	1516:1536	a "trapped structure" (phase behaviour)	1516:1554	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	9	75	theme	phase	1539:1543	arg1	behaviour					1545:1553	phase behaviour	1539:1553	phase behaviour	1539:1553	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	1	76	dep	properties	182:191	arg1	the					169:171	the	169:171	the	169:171	The effects of three polysaccharides on the textural properties and microstructure of myofibrillar protein (MP) gels were studied.					
32807542	0	77	theme	gelation	44:51	arg1	properties					53:62	the gelation properties	40:62	the gelation properties of myofibrillar protein	40:86	The effects of three polysaccharides on the gelation properties of myofibrillar protein: Phase behaviour and moisture stability.					
32807542	3	78	theme	thermal	625:631	arg1	process					633:639	the thermal process	621:639	the thermal process	621:639	The SEM images indicated that moisture extrusion formed moisture channels and deteriorated the aggregation of MP gel networks during the thermal process.					
32807542	4	79	theme	compact	785:791	arg1	networks					810:817	compact and integral gel networks	785:817	compact and integral gel networks	785:817	The polysaccharides stabilized moisture and reduced the appearance of moisture channels in the gel network, thereby promoting the formation of compact and integral gel networks.					
32807542	2	80	contain	had	452:454	arg1	KG					448:449	KG	448:449	KG	448:449	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	2	80	contain	had	452:454	arg1	glucomannan					435:445	konjac glucomannan	428:445	konjac glucomannan (KG)	428:450	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	2	80	contain	had	452:454	arg2	effects					464:470	limited effects	456:470	limited effects	456:470	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	9	81	theme	ideal	1560:1564	arg1	polysaccharides					1453:1467	polysaccharides	1453:1467	polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour)	1453:1554	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	9	81	theme	ideal	1560:1564	arg1	DF					1634:1635	DF	1634:1635	especially DF	1623:1635	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	9	81	theme	ideal	1560:1564	arg1	replacements					1570:1581	ideal fat replacements	1560:1581	ideal fat replacements for improving composite gel properties	1560:1620	The study indicated that polysaccharides that contribute to moisture stability and form a "trapped structure" (phase behaviour) are ideal fat replacements for improving composite gel properties, especially DF.					
32807542	2	82	dep	fibre	376:380	arg1	addition					412:419	addition	412:419	addition	412:419	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	2	83	theme	MP	317:318	arg1	gels					320:323	composite MP gels	307:323	composite MP gels	307:323	The gel strength and rheological properties of composite MP gels were significantly improved with insoluble dietary fibre (DF) and modified starch (MS) addition, while konjac glucomannan (KG) had limited effects at 1% addition.					
32807542	5	84	theme	trapped	949:955	arg1	forms					908:912	two main forms	899:912	two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure	899:1005	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	5	84	theme	trapped	949:955	arg1	structure					958:966	1) the "trapped" structure	941:966	1) the "trapped" structure	941:966	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32807542	7	85	theme	polysaccharide	1151:1164	arg1	hydrogel					1166:1173	the continuous polysaccharide hydrogel	1136:1173	the continuous polysaccharide hydrogel	1136:1173	In the "interpenetrated" structure, the continuous polysaccharide hydrogel substantially hindered the aggregation of MP gel networks.					
32807542	5	86	dep	structure	997:1005	arg1	2					972:972	2	972:972	2	972:972	The MP-polysaccharide mixture is a thermally incompatible system and presented two main forms after the thermal process: 1) the "trapped" structure and 2) the "interpenetrated" structure.					
32912690	1	0	theme	proliferation	327:339	arg1	inhibition					302:311	the inhibition	298:311	the inhibition of tumor cell proliferation	298:339	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	1	0	theme	proliferation	327:339	arg1	neuroprotection					277:291	neuroprotection	277:291	neuroprotection	277:291	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	1	0	theme	proliferation	327:339	arg1	activity					267:274	antioxidant activity	255:274	antioxidant activity	255:274	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	5	1	theme	NMR	860:862	arg1	analysis					864:871	NMR analysis	860:871	NMR analysis	860:871	Novel C-Gs were purified by HPLC, and their chemical structures were determined using NMR analysis.					
32912690	0	2	theme	crocins	78:84	arg1	capacity					44:51	antioxidant capacity	32:51	antioxidant capacity	32:51	Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.					
32912690	0	2	theme	crocins	78:84	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of neuroprotection	0:29	Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.					
32912690	0	2	theme	crocins	78:84	arg1	water-solubility					58:73	water-solubility	58:73	water-solubility of crocins by transglucosylation	58:106	Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.					
32912690	6	3	theme	Novel	874:878	arg1	C-Gs					880:883	Novel C-Gs	874:883	Novel C-Gs	874:883	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	7	4	theme	novel	1202:1206	arg1	C-Gs					1208:1211	novel C-Gs	1202:1211	novel C-Gs	1202:1211	This advanced neuroprotection of novel C-Gs could be highly associated with their enhanced antioxidant activity.					
32912690	6	5	theme	cell	1145:1148	arg1	%					1165:1165	cell viability 84.6 %	1145:1165	cell viability 84.6 %	1145:1165	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	6	5	theme	cell	1145:1148	arg1	crocin					1137:1142	crocin	1137:1142	crocin (cell viability 84.6 %)	1137:1166	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	8	6	theme	neuroprotection	1389:1403	arg1	efficacy					1377:1384	better clinical efficacy	1361:1384	better clinical efficacy of neuroprotection than trans-crocin	1361:1421	Thus, the enhanced water solubility and functionality of novel C-Gs can induce better clinical efficacy of neuroprotection than trans-crocin.					
32912690	1	7	theme	antioxidant	255:265	arg1	activity					267:274	antioxidant activity	255:274	antioxidant activity	255:274	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	8	8	theme	enhanced	1292:1299	arg1	solubility					1307:1316	water solubility	1301:1316	water solubility	1301:1316	Thus, the enhanced water solubility and functionality of novel C-Gs can induce better clinical efficacy of neuroprotection than trans-crocin.					
32912690	6	9	theme	hippocampal	1071:1081	arg1	cells					1092:1096	HT22 mouse hippocampal neuronal cells	1060:1096	HT22 mouse hippocampal neuronal cells	1060:1096	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	8	10	theme	better	1361:1366	arg1	efficacy					1377:1384	better clinical efficacy	1361:1384	better clinical efficacy of neuroprotection than trans-crocin	1361:1421	Thus, the enhanced water solubility and functionality of novel C-Gs can induce better clinical efficacy of neuroprotection than trans-crocin.					
32912690	3	11	theme	pressure	528:535	arg1	technique					543:551	High hydrostatic pressure (HHP) technique	511:551	High hydrostatic pressure (HHP) technique	511:551	High hydrostatic pressure (HHP) technique was applied to the synthesis process of C-Gs in order to improve its transglucosylation yield.					
32912690	4	12	theme	MPa	654:656	arg1	condition					662:670	A 100 MPa HHP condition	648:670	A 100 MPa HHP condition	648:670	A 100 MPa HHP condition enhanced the production yield of C-Gs by 1.95 times compared to that of 0.1 MPa atmospheric pressure.					
32912690	6	13	theme	%	1054:1054	arg1	viability					1033:1041	cell viability	1028:1041	cell viability 92.5-100.4 %	1028:1054	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	6	14	dep	neuroprotections	1010:1025	arg1	viability					1033:1041	cell viability	1028:1041	cell viability 92.5-100.4 %	1028:1054	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	2	15	theme	crocin	383:388	arg1	functionality					366:378	the functionality	362:378	the functionality of crocin	362:388	In order to improve the functionality of crocin, α-glucosyl-(1→6)-trans-crocins (C-Gs) were synthesized using sucrose and dextransucrase from Leuconostoc mesenteroides.					
32912690	5	16	theme	Novel	774:778	arg1	C-Gs					780:783	Novel C-Gs	774:783	Novel C-Gs	774:783	Novel C-Gs were purified by HPLC, and their chemical structures were determined using NMR analysis.					
32912690	7	17	theme	advanced	1174:1181	arg1	neuroprotection					1183:1197	This advanced neuroprotection	1169:1197	This advanced neuroprotection of novel C-Gs	1169:1211	This advanced neuroprotection of novel C-Gs could be highly associated with their enhanced antioxidant activity.					
32912690	1	18	theme	carotenoid	187:196	arg1	pigments					198:205	the major carotenoid pigments	177:205	the major carotenoid pigments of Crocus sativus (saffron)	177:233	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	6	19	theme	water	895:899	arg1	solubility					901:910	water solubility	895:910	water solubility	895:910	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	5	20	theme	chemical	818:825	arg1	structures					827:836	their chemical structures	812:836	their chemical structures	812:836	Novel C-Gs were purified by HPLC, and their chemical structures were determined using NMR analysis.					
32912690	1	21	theme	pigments	198:205	arg1	pigments					198:205	the major carotenoid pigments	177:205	the major carotenoid pigments of Crocus sativus (saffron)	177:233	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	1	21	theme	pigments	198:205	arg1	one					170:172	one	170:172	one	170:172	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	1	21	theme	pigments	198:205	arg1	Crocin					162:167	Crocin	162:167	Crocin	162:167	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	6	22	theme	92.5-100.4	1043:1052	arg1	%					1054:1054	%	1054:1054	%	1054:1054	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	0	23	theme	hydrostatic	140:150	arg1	pressure					152:159	high hydrostatic pressure	135:159	high hydrostatic pressure	135:159	Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.					
32912690	6	24	from	neuroprotections	1010:1025	arg1	cells					1092:1096	HT22 mouse hippocampal neuronal cells	1060:1096	HT22 mouse hippocampal neuronal cells	1060:1096	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	8	25	theme	C-Gs	1345:1348	arg1	solubility					1307:1316	water solubility	1301:1316	water solubility	1301:1316	Thus, the enhanced water solubility and functionality of novel C-Gs can induce better clinical efficacy of neuroprotection than trans-crocin.					
32912690	8	25	theme	C-Gs	1345:1348	arg1	functionality					1322:1334	functionality	1322:1334	functionality	1322:1334	Thus, the enhanced water solubility and functionality of novel C-Gs can induce better clinical efficacy of neuroprotection than trans-crocin.					
32912690	7	26	theme	antioxidant	1260:1270	arg1	activity					1272:1279	their enhanced antioxidant activity	1245:1279	their enhanced antioxidant activity	1245:1279	This advanced neuroprotection of novel C-Gs could be highly associated with their enhanced antioxidant activity.					
32912690	0	27	theme	neuroprotection	15:29	arg1	capacity					44:51	antioxidant capacity	32:51	antioxidant capacity	32:51	Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.					
32912690	0	27	theme	neuroprotection	15:29	arg1	Enhancement					0:10	Enhancement	0:10	Enhancement of neuroprotection	0:29	Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.					
32912690	0	27	theme	neuroprotection	15:29	arg1	water-solubility					58:73	water-solubility	58:73	water-solubility of crocins by transglucosylation	58:106	Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.					
32912690	6	28	theme	antioxidant	930:940	arg1	activity					942:949	antioxidant activity	930:949	antioxidant activity	930:949	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	0	29	theme	high	135:138	arg1	pressure					152:159	high hydrostatic pressure	135:159	high hydrostatic pressure	135:159	Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.					
32912690	3	30	theme	transglucosylation	622:639	arg1	yield					641:645	its transglucosylation yield	618:645	its transglucosylation yield	618:645	High hydrostatic pressure (HHP) technique was applied to the synthesis process of C-Gs in order to improve its transglucosylation yield.					
32912690	8	31	theme	novel	1339:1343	arg1	C-Gs					1345:1348	novel C-Gs	1339:1348	novel C-Gs	1339:1348	Thus, the enhanced water solubility and functionality of novel C-Gs can induce better clinical efficacy of neuroprotection than trans-crocin.					
32912690	1	32	theme	Crocus	210:215	arg1	saffron					226:232	saffron	226:232	saffron	226:232	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	1	32	theme	Crocus	210:215	arg1	sativus					217:223	Crocus sativus	210:223	Crocus sativus (saffron)	210:233	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	7	33	theme	enhanced	1251:1258	arg1	activity					1272:1279	their enhanced antioxidant activity	1245:1279	their enhanced antioxidant activity	1245:1279	This advanced neuroprotection of novel C-Gs could be highly associated with their enhanced antioxidant activity.					
32912690	7	34	theme	C-Gs	1208:1211	arg1	neuroprotection					1183:1197	This advanced neuroprotection	1169:1197	This advanced neuroprotection of novel C-Gs	1169:1211	This advanced neuroprotection of novel C-Gs could be highly associated with their enhanced antioxidant activity.					
32912690	4	35	theme	100	650:652	arg1	MPa					654:656	MPa	654:656	MPa	654:656	A 100 MPa HHP condition enhanced the production yield of C-Gs by 1.95 times compared to that of 0.1 MPa atmospheric pressure.					
32912690	1	36	theme	sativus	217:223	arg1	pigments					198:205	the major carotenoid pigments	177:205	the major carotenoid pigments of Crocus sativus (saffron)	177:233	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	0	37	theme	antioxidant	32:42	arg1	capacity					44:51	antioxidant capacity	32:51	antioxidant capacity	32:51	Enhancement of neuroprotection, antioxidant capacity, and water-solubility of crocins by transglucosylation using dextransucrase under high hydrostatic pressure.					
32912690	6	38	dep	times	920:924	arg1	times					959:963	1.5-2.6 times	951:963	1.5-2.6 times	951:963	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	2	39	theme	α-glucosyl-	391:401	arg1	C-Gs					423:426	C-Gs	423:426	C-Gs	423:426	In order to improve the functionality of crocin, α-glucosyl-(1→6)-trans-crocins (C-Gs) were synthesized using sucrose and dextransucrase from Leuconostoc mesenteroides.					
32912690	2	39	theme	α-glucosyl-	391:401	arg1	-trans-crocins					407:420	α-glucosyl-(1→6)-trans-crocins	391:420	α-glucosyl-(1→6)-trans-crocins (C-Gs)	391:427	In order to improve the functionality of crocin, α-glucosyl-(1→6)-trans-crocins (C-Gs) were synthesized using sucrose and dextransucrase from Leuconostoc mesenteroides.					
32912690	8	40	theme	water	1301:1305	arg1	solubility					1307:1316	water solubility	1301:1316	water solubility	1301:1316	Thus, the enhanced water solubility and functionality of novel C-Gs can induce better clinical efficacy of neuroprotection than trans-crocin.					
32912690	8	41	theme	clinical	1368:1375	arg1	efficacy					1377:1384	better clinical efficacy	1361:1384	better clinical efficacy of neuroprotection than trans-crocin	1361:1421	Thus, the enhanced water solubility and functionality of novel C-Gs can induce better clinical efficacy of neuroprotection than trans-crocin.					
32912690	6	42	theme	mouse	1065:1069	arg1	cells					1092:1096	HT22 mouse hippocampal neuronal cells	1060:1096	HT22 mouse hippocampal neuronal cells	1060:1096	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	6	43	theme	cell	1028:1031	arg1	viability					1033:1041	cell viability	1028:1041	cell viability 92.5-100.4 %	1028:1054	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	3	44	theme	synthesis	572:580	arg1	process					582:588	the synthesis process	568:588	the synthesis process of C-Gs	568:596	High hydrostatic pressure (HHP) technique was applied to the synthesis process of C-Gs in order to improve its transglucosylation yield.					
32912690	4	45	theme	production	685:694	arg1	yield					696:700	the production yield	681:700	the production yield of C-Gs	681:708	A 100 MPa HHP condition enhanced the production yield of C-Gs by 1.95 times compared to that of 0.1 MPa atmospheric pressure.					
32912690	1	46	theme	tumor	316:320	arg1	proliferation					327:339	tumor cell proliferation	316:339	tumor cell proliferation	316:339	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	4	47	theme	atmospheric	752:762	arg1	pressure					764:771	0.1 MPa atmospheric pressure	744:771	0.1 MPa atmospheric pressure	744:771	A 100 MPa HHP condition enhanced the production yield of C-Gs by 1.95 times compared to that of 0.1 MPa atmospheric pressure.					
32912690	4	48	theme	C-Gs	705:708	arg1	yield					696:700	the production yield	681:700	the production yield of C-Gs	681:708	A 100 MPa HHP condition enhanced the production yield of C-Gs by 1.95 times compared to that of 0.1 MPa atmospheric pressure.					
32912690	4	49	theme	0.1	744:746	arg1	MPa					748:750	MPa	748:750	MPa	748:750	A 100 MPa HHP condition enhanced the production yield of C-Gs by 1.95 times compared to that of 0.1 MPa atmospheric pressure.					
32912690	4	50	theme	MPa	748:750	arg1	pressure					764:771	0.1 MPa atmospheric pressure	744:771	0.1 MPa atmospheric pressure	744:771	A 100 MPa HHP condition enhanced the production yield of C-Gs by 1.95 times compared to that of 0.1 MPa atmospheric pressure.					
32912690	4	51	theme	HHP	658:660	arg1	condition					662:670	A 100 MPa HHP condition	648:670	A 100 MPa HHP condition	648:670	A 100 MPa HHP condition enhanced the production yield of C-Gs by 1.95 times compared to that of 0.1 MPa atmospheric pressure.					
32912690	6	52	theme	neuronal	1083:1090	arg1	cells					1092:1096	HT22 mouse hippocampal neuronal cells	1060:1096	HT22 mouse hippocampal neuronal cells	1060:1096	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	2	53	theme	Leuconostoc	484:494	arg1	mesenteroides					496:508	Leuconostoc mesenteroides	484:508	Leuconostoc mesenteroides	484:508	In order to improve the functionality of crocin, α-glucosyl-(1→6)-trans-crocins (C-Gs) were synthesized using sucrose and dextransucrase from Leuconostoc mesenteroides.					
32912690	6	54	theme	HT22	1060:1063	arg1	cells					1092:1096	HT22 mouse hippocampal neuronal cells	1060:1096	HT22 mouse hippocampal neuronal cells	1060:1096	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	3	55	theme	C-Gs	593:596	arg1	process					582:588	the synthesis process	568:588	the synthesis process of C-Gs	568:596	High hydrostatic pressure (HHP) technique was applied to the synthesis process of C-Gs in order to improve its transglucosylation yield.					
32912690	3	56	theme	High	511:514	arg1	HHP					538:540	HHP	538:540	HHP	538:540	High hydrostatic pressure (HHP) technique was applied to the synthesis process of C-Gs in order to improve its transglucosylation yield.					
32912690	3	56	theme	High	511:514	arg1	pressure					528:535	High hydrostatic pressure	511:535	High hydrostatic pressure (HHP) technique	511:551	High hydrostatic pressure (HHP) technique was applied to the synthesis process of C-Gs in order to improve its transglucosylation yield.					
32912690	1	57	theme	major	181:185	arg1	pigments					198:205	the major carotenoid pigments	177:205	the major carotenoid pigments of Crocus sativus (saffron)	177:233	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	6	58	dep	increased	885:893	arg1	compared					980:987	compared	980:987	compared to crocin	980:997	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	1	59	theme	cell	322:325	arg1	proliferation					327:339	tumor cell proliferation	316:339	tumor cell proliferation	316:339	Crocin, one of the major carotenoid pigments of Crocus sativus (saffron), is responsible for antioxidant activity, neuroprotection, and the inhibition of tumor cell proliferation.					
32912690	6	60	theme	viability	1150:1158	arg1	%					1165:1165	cell viability 84.6 %	1145:1165	cell viability 84.6 %	1145:1165	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	6	60	theme	viability	1150:1158	arg1	crocin					1137:1142	crocin	1137:1142	crocin (cell viability 84.6 %)	1137:1166	Novel C-Gs increased water solubility 4.6-5.7 times and antioxidant activity 1.5-2.6 times, respectively, compared to crocin, and their neuroprotections (cell viability 92.5-100.4 %) on HT22 mouse hippocampal neuronal cells were significantly higher than that of crocin (cell viability 84.6 %).					
32912690	3	61	theme	hydrostatic	516:526	arg1	HHP					538:540	HHP	538:540	HHP	538:540	High hydrostatic pressure (HHP) technique was applied to the synthesis process of C-Gs in order to improve its transglucosylation yield.					
32912690	3	61	theme	hydrostatic	516:526	arg1	pressure					528:535	High hydrostatic pressure	511:535	High hydrostatic pressure (HHP) technique	511:551	High hydrostatic pressure (HHP) technique was applied to the synthesis process of C-Gs in order to improve its transglucosylation yield.					
34044919	5	0	from	effect	962:967	arg1	healing					978:984	wound healing	972:984	wound healing	972:984	Moreover, the hydrogel showed a promoting effect on wound healing in a rat scald model.					
34044919	3	1	theme	vapor	660:664	arg1	permeability					666:677	its water vapor permeability	650:677	its water vapor permeability	650:677	The porosity and swelling degree of SA-COS-ZnO hydrogel are 80% and 150%, respectively, and its water vapor permeability is 682 g/m2/24h.					
34044919	2	2	theme	sodium	393:398	arg1	alginate					400:407	aldehydated sodium alginate	381:407	aldehydated sodium alginate (SA)	381:412	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	2	2	theme	sodium	393:398	arg1	SA					410:411	SA	410:411	SA	410:411	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	5	3	theme	wound	972:976	arg1	healing					978:984	wound healing	972:984	wound healing	972:984	Moreover, the hydrogel showed a promoting effect on wound healing in a rat scald model.					
34044919	5	4	theme	scald	995:999	arg1	model					1001:1005	a rat scald model	989:1005	a rat scald model	989:1005	Moreover, the hydrogel showed a promoting effect on wound healing in a rat scald model.					
34044919	2	5	theme	antibacterial	513:525	arg1	environment					527:537	a moist and antibacterial environment	501:537	a moist and antibacterial environment for wound healing	501:555	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	4	6	theme	blood	751:755	arg1	cells					757:761	blood cells	751:761	blood cells	751:761	The composite hydrogel showed good biocompatibility to blood cells, 3T3 cells, and 293T cells, and significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis.					
34044919	2	7	theme	composite	319:327	arg1	hydrogel					329:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel	246:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction	246:372	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	4	8	theme	good	726:729	arg1	biocompatibility					731:746	good biocompatibility	726:746	good biocompatibility to blood cells, 3T3 cells, and 293T cells	726:788	The composite hydrogel showed good biocompatibility to blood cells, 3T3 cells, and 293T cells, and significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis.					
34044919	4	9	theme	3T3	764:766	arg1	cells					768:772	3T3 cells	764:772	3T3 cells	764:772	The composite hydrogel showed good biocompatibility to blood cells, 3T3 cells, and 293T cells, and significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis.					
34044919	6	10	theme	carbohydrates	1047:1059	arg1	hydrogels					1071:1079	marine carbohydrates composite hydrogels	1040:1079	marine carbohydrates composite hydrogels	1040:1079	The present study suggests that marine carbohydrates composite hydrogels are promising in wound care management.					
34044919	2	11	theme	moist	503:507	arg1	environment					527:537	a moist and antibacterial environment	501:537	a moist and antibacterial environment for wound healing	501:555	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	4	12	theme	antibacterial	807:819	arg1	activity					821:828	significant antibacterial activity	795:828	significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis	795:917	The composite hydrogel showed good biocompatibility to blood cells, 3T3 cells, and 293T cells, and significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis.					
34044919	1	13	dep	cell	154:157	arg1	proliferation					159:171	proliferation	159:171	proliferation	159:171	Moist, breathable and antibacterial microenvironment can promote cell proliferation and migration, which is beneficial to wound healing.					
34044919	1	13	dep	cell	154:157	arg1	migration					177:185	migration	177:185	migration	177:185	Moist, breathable and antibacterial microenvironment can promote cell proliferation and migration, which is beneficial to wound healing.					
34044919	6	14	theme	composite	1061:1069	arg1	hydrogels					1071:1079	marine carbohydrates composite hydrogels	1040:1079	marine carbohydrates composite hydrogels	1040:1079	The present study suggests that marine carbohydrates composite hydrogels are promising in wound care management.					
34044919	2	15	theme	chitosan	415:422	arg1	COS					441:443	COS	441:443	COS	441:443	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	2	15	theme	chitosan	415:422	arg1	oligosaccharide					424:438	chitosan oligosaccharide	415:438	chitosan oligosaccharide (COS)	415:444	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	5	16	theme	rat	991:993	arg1	model					1001:1005	a rat scald model	989:1005	a rat scald model	989:1005	Moreover, the hydrogel showed a promoting effect on wound healing in a rat scald model.					
34044919	2	17	theme	base	360:363	arg1	reaction					365:372	spontaneous Schiff base reaction	341:372	spontaneous Schiff base reaction	341:372	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	1	18	theme	wound	211:215	arg1	healing					217:223	wound healing	211:223	wound healing	211:223	Moist, breathable and antibacterial microenvironment can promote cell proliferation and migration, which is beneficial to wound healing.					
34044919	2	19	theme	oxide	456:460	arg1	nanoparticles					468:480	zinc oxide (ZnO) nanoparticles	451:480	zinc oxide (ZnO) nanoparticles	451:480	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	2	20	theme	Schiff	353:358	arg1	reaction					365:372	spontaneous Schiff base reaction	341:372	spontaneous Schiff base reaction	341:372	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	2	21	theme	zinc	451:454	arg1	oxide					456:460	zinc oxide	451:460	zinc oxide (ZnO) nanoparticles	451:480	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	2	21	theme	zinc	451:454	arg1	ZnO					463:465	ZnO	463:465	ZnO	463:465	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	4	22	theme	293T	779:782	arg1	cells					784:788	293T cells	779:788	293T cells	779:788	The composite hydrogel showed good biocompatibility to blood cells, 3T3 cells, and 293T cells, and significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis.					
34044919	2	23	theme	spontaneous	341:351	arg1	reaction					365:372	spontaneous Schiff base reaction	341:372	spontaneous Schiff base reaction	341:372	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	3	24	theme	SA-COS-ZnO	594:603	arg1	hydrogel					605:612	SA-COS-ZnO hydrogel	594:612	SA-COS-ZnO hydrogel	594:612	The porosity and swelling degree of SA-COS-ZnO hydrogel are 80% and 150%, respectively, and its water vapor permeability is 682 g/m2/24h.					
34044919	1	25	theme	antibacterial	111:123	arg1	microenvironment					125:140	antibacterial microenvironment	111:140	antibacterial microenvironment	111:140	Moist, breathable and antibacterial microenvironment can promote cell proliferation and migration, which is beneficial to wound healing.					
34044919	5	26	theme	promoting	952:960	arg1	effect					962:967	a promoting effect	950:967	a promoting effect on wound healing	950:984	Moreover, the hydrogel showed a promoting effect on wound healing in a rat scald model.					
34044919	3	27	theme	porosity	562:569	arg1	degree					584:589	The porosity and swelling degree	558:589	The porosity and swelling degree of SA-COS-ZnO hydrogel	558:612	The porosity and swelling degree of SA-COS-ZnO hydrogel are 80% and 150%, respectively, and its water vapor permeability is 682 g/m2/24h.					
34044919	3	27	theme	porosity	562:569	arg1	%					620:620	80%	618:620	80%	618:620	The porosity and swelling degree of SA-COS-ZnO hydrogel are 80% and 150%, respectively, and its water vapor permeability is 682 g/m2/24h.					
34044919	6	28	theme	present	1012:1018	arg1	study					1020:1024	The present study	1008:1024	The present study	1008:1024	The present study suggests that marine carbohydrates composite hydrogels are promising in wound care management.					
34044919	2	29	theme	wound	543:547	arg1	healing					549:555	wound healing	543:555	wound healing	543:555	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	2	30	theme	sodium	254:259	arg1	hydrogel					329:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel	246:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction	246:372	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	0	31	theme	composite	38:46	arg1	hydrogel					48:55	composite hydrogel	38:55	composite hydrogel	38:55	Alginate-chitosan oligosaccharide-ZnO composite hydrogel for accelerating wound healing.					
34044919	2	32	theme	novel	248:252	arg1	hydrogel					329:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel	246:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction	246:372	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	4	33	theme	Candida	879:885	arg1	albicans					887:894	Candida albicans	879:894	Candida albicans	879:894	The composite hydrogel showed good biocompatibility to blood cells, 3T3 cells, and 293T cells, and significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis.					
34044919	4	34	theme	significant	795:805	arg1	activity					821:828	significant antibacterial activity	795:828	significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis	795:917	The composite hydrogel showed good biocompatibility to blood cells, 3T3 cells, and 293T cells, and significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis.					
34044919	2	35	theme	aldehydated	381:391	arg1	alginate					400:407	aldehydated sodium alginate	381:407	aldehydated sodium alginate (SA)	381:412	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	2	35	theme	aldehydated	381:391	arg1	SA					410:411	SA	410:411	SA	410:411	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	0	36	theme	wound	74:78	arg1	healing					80:86	wound healing	74:86	wound healing	74:86	Alginate-chitosan oligosaccharide-ZnO composite hydrogel for accelerating wound healing.					
34044919	6	37	from	management	1109:1118	arg1	promising					1085:1093	promising	1085:1093	promising	1085:1093	The present study suggests that marine carbohydrates composite hydrogels are promising in wound care management.					
34044919	6	38	theme	care	1104:1107	arg1	management					1109:1118	wound care management	1098:1118	wound care management	1098:1118	The present study suggests that marine carbohydrates composite hydrogels are promising in wound care management.					
34044919	3	39	theme	water	654:658	arg1	permeability					666:677	its water vapor permeability	650:677	its water vapor permeability	650:677	The porosity and swelling degree of SA-COS-ZnO hydrogel are 80% and 150%, respectively, and its water vapor permeability is 682 g/m2/24h.					
34044919	3	40	theme	hydrogel	605:612	arg1	degree					584:589	The porosity and swelling degree	558:589	The porosity and swelling degree of SA-COS-ZnO hydrogel	558:612	The porosity and swelling degree of SA-COS-ZnO hydrogel are 80% and 150%, respectively, and its water vapor permeability is 682 g/m2/24h.					
34044919	3	40	theme	hydrogel	605:612	arg1	%					620:620	80%	618:620	80%	618:620	The porosity and swelling degree of SA-COS-ZnO hydrogel are 80% and 150%, respectively, and its water vapor permeability is 682 g/m2/24h.					
34044919	6	41	theme	wound	1098:1102	arg1	management					1109:1118	wound care management	1098:1118	wound care management	1098:1118	The present study suggests that marine carbohydrates composite hydrogels are promising in wound care management.					
34044919	2	42	theme	SA-COS-ZnO	307:316	arg1	hydrogel					329:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel	246:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction	246:372	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	2	43	theme	oxide	300:304	arg1	hydrogel					329:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel	246:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction	246:372	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	4	44	theme	composite	700:708	arg1	hydrogel					710:717	The composite hydrogel	696:717	The composite hydrogel	696:717	The composite hydrogel showed good biocompatibility to blood cells, 3T3 cells, and 293T cells, and significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis.					
34044919	2	45	theme	oligosaccharide‑zinc	279:298	arg1	hydrogel					329:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel	246:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction	246:372	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	6	46	theme	marine	1040:1045	arg1	hydrogels					1071:1079	marine carbohydrates composite hydrogels	1040:1079	marine carbohydrates composite hydrogels	1040:1079	The present study suggests that marine carbohydrates composite hydrogels are promising in wound care management.					
34044919	2	47	theme	alginate-chitosan	261:277	arg1	hydrogel					329:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel	246:336	a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction	246:372	Here, we fabricated a novel sodium alginate-chitosan oligosaccharide‑zinc oxide (SA-COS-ZnO) composite hydrogel by spontaneous Schiff base reaction, using aldehydated sodium alginate (SA), chitosan oligosaccharide (COS), and zinc oxide (ZnO) nanoparticles, which can provide a moist and antibacterial environment for wound healing.					
34044919	6	48	from	promising	1085:1093	arg1	management					1109:1118	wound care management	1098:1118	wound care management	1098:1118	The present study suggests that marine carbohydrates composite hydrogels are promising in wound care management.					
34044919	3	49	theme	swelling	575:582	arg1	degree					584:589	The porosity and swelling degree	558:589	The porosity and swelling degree of SA-COS-ZnO hydrogel	558:612	The porosity and swelling degree of SA-COS-ZnO hydrogel are 80% and 150%, respectively, and its water vapor permeability is 682 g/m2/24h.					
34044919	3	49	theme	swelling	575:582	arg1	%					620:620	80%	618:620	80%	618:620	The porosity and swelling degree of SA-COS-ZnO hydrogel are 80% and 150%, respectively, and its water vapor permeability is 682 g/m2/24h.					
32102878	12	0	theme	synthetic	2013:2021	arg1	formulations					2023:2034	synthetic formulations	2013:2034	synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition	2013:2103	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	6	1	theme	seminal	896:902	arg1	plasma					904:909	whole human seminal plasma	884:909	whole human seminal plasma	884:909	Infection and inhibition in whole human seminal plasma are accurately mimicked by a stable synthetic simulant of seminal fluid that we formulated.					
32102878	7	2	theme	small-solute	1098:1109	arg1	composition					1111:1121	their small-solute composition	1092:1121	their small-solute composition	1092:1121	Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies.IMPORTANCE Biological secretions allow viruses to spread between individuals.					
32102878	3	3	from	inhibition	461:470	arg1	medium					480:485	this medium	475:485	this medium	475:485	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	11	4	theme	sexual	1833:1838	arg1	transmission					1840:1851	the sexual transmission	1829:1851	the sexual transmission of HIV-1	1829:1860	This effect of semen fructose likely reduces the efficacy of such inhibitors to prevent the sexual transmission of HIV-1.					
32102878	6	5	theme	whole	884:888	arg1	plasma					904:909	whole human seminal plasma	884:909	whole human seminal plasma	884:909	Infection and inhibition in whole human seminal plasma are accurately mimicked by a stable synthetic simulant of seminal fluid that we formulated.					
32102878	4	6	theme	antibodies	551:560	arg1	potency					540:546	the potency	536:546	the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1	536:643	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	6	7	from	Infection	856:864	arg1	plasma					904:909	whole human seminal plasma	884:909	whole human seminal plasma	884:909	Infection and inhibition in whole human seminal plasma are accurately mimicked by a stable synthetic simulant of seminal fluid that we formulated.					
32102878	12	8	theme	in	1990:1991	arg1	assessment					1999:2008	their in vitro assessment	1984:2008	their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition	1984:2103	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	10	9	from	fructose	1591:1598	arg1	semen					1603:1607	semen	1603:1607	semen	1603:1607	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	6	10	from	inhibition	870:879	arg1	plasma					904:909	whole human seminal plasma	884:909	whole human seminal plasma	884:909	Infection and inhibition in whole human seminal plasma are accurately mimicked by a stable synthetic simulant of seminal fluid that we formulated.					
32102878	7	11	theme	biological	1069:1078	arg1	secretions					1080:1089	biological secretions	1069:1089	biological secretions	1069:1089	Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies.IMPORTANCE Biological secretions allow viruses to spread between individuals.					
32102878	12	12	theme	antibodies	1953:1962	arg1	evaluation					1905:1914	the preclinical evaluation	1889:1914	the preclinical evaluation of microbicides and vaccine-elicited antibodies	1889:1962	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	12	13	from	assessment	1999:2008	arg1	formulations					2023:2034	synthetic formulations	2013:2034	synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition	2013:2103	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	11	14	theme	fructose	1762:1769	arg1	effect					1746:1751	This effect	1741:1751	This effect of semen fructose	1741:1769	This effect of semen fructose likely reduces the efficacy of such inhibitors to prevent the sexual transmission of HIV-1.					
32102878	3	15	from	infection	447:455	arg1	medium					480:485	this medium	475:485	this medium	475:485	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	1	16	theme	pathogenic	183:192	arg1	viruses					194:200	various pathogenic viruses	175:200	various pathogenic viruses	175:200	Semen is the primary transmission vehicle for various pathogenic viruses.					
32102878	6	17	theme	synthetic	947:955	arg1	simulant					957:964	a stable synthetic simulant	938:964	a stable synthetic simulant of seminal fluid that we formulated	938:1000	Infection and inhibition in whole human seminal plasma are accurately mimicked by a stable synthetic simulant of seminal fluid that we formulated.					
32102878	10	18	from	sugars	1700:1705	arg1	protein					1723:1729	the envelope protein	1710:1729	the envelope protein of HIV-1	1710:1738	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	5	19	theme	high	666:669	arg1	concentration					671:683	The extraordinarily high concentration	646:683	The extraordinarily high concentration of the monosaccharide fructose in semen	646:723	The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env.					
32102878	10	20	theme	HIV-1	1734:1738	arg1	protein					1723:1729	the envelope protein	1710:1729	the envelope protein of HIV-1	1710:1738	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	8	21	theme	process	1429:1435	arg1	inhibition					1410:1419	inhibition	1410:1419	inhibition of this process	1410:1435	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	8	21	theme	process	1429:1435	arg1	potential					1383:1391	the infectivity potential	1367:1391	the infectivity potential of the virus	1367:1404	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	4	22	theme	envelope	606:613	arg1	Envs					630:633	Envs	630:633	Envs	630:633	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	4	22	theme	envelope	606:613	arg1	glycoproteins					615:627	the envelope glycoproteins	602:627	the envelope glycoproteins (Envs) of HIV-1	602:643	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	7	23	dep	content	1058:1064	arg1	addition					1034:1041	addition	1034:1041	addition	1034:1041	Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies.IMPORTANCE Biological secretions allow viruses to spread between individuals.					
32102878	5	24	theme	mannose	831:837	arg1	residues					839:846	α1,2-linked mannose residues	819:846	α1,2-linked mannose residues on Env	819:853	The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env.					
32102878	8	25	theme	unique	1299:1304	arg1	composition					1306:1316	a unique composition	1297:1316	a unique composition	1297:1316	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	9	26	theme	whole	1490:1494	arg1	plasma					1510:1515	whole human seminal plasma	1490:1515	whole human seminal plasma	1490:1515	Here, we describe HIV-1 infection and inhibition in whole human seminal plasma and a synthetic simulant that we formulated.					
32102878	4	27	theme	HIV-1	639:643	arg1	Envs					630:633	Envs	630:633	Envs	630:633	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	4	27	theme	HIV-1	639:643	arg1	glycoproteins					615:627	the envelope glycoproteins	602:627	the envelope glycoproteins (Envs) of HIV-1	602:643	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	5	28	from	residues	839:846	arg1	Env					851:853	Env	851:853	Env	851:853	The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env.					
32102878	9	29	theme	seminal	1502:1508	arg1	plasma					1510:1515	whole human seminal plasma	1490:1515	whole human seminal plasma	1490:1515	Here, we describe HIV-1 infection and inhibition in whole human seminal plasma and a synthetic simulant that we formulated.					
32102878	0	30	from	Content	9:15	arg1	Semen					38:42	Human Semen	32:42	Human Semen	32:42	The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans.					
32102878	0	31	theme	Broad	67:71	arg1	Antivirals					84:93	Broad and Potent Antivirals	67:93	Broad and Potent Antivirals That Target High-Mannose Glycans	67:126	The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans.					
32102878	1	32	theme	transmission	150:161	arg1	vehicle					163:169	the primary transmission vehicle	138:169	the primary transmission vehicle for various pathogenic viruses	138:200	Semen is the primary transmission vehicle for various pathogenic viruses.					
32102878	1	32	theme	transmission	150:161	arg1	Semen					129:133	Semen	129:133	Semen	129:133	Semen is the primary transmission vehicle for various pathogenic viruses.					
32102878	6	33	theme	fluid	977:981	arg1	simulant					957:964	a stable synthetic simulant	938:964	a stable synthetic simulant of seminal fluid that we formulated	938:1000	Infection and inhibition in whole human seminal plasma are accurately mimicked by a stable synthetic simulant of seminal fluid that we formulated.					
32102878	8	34	theme	sugars	1342:1347	arg1	composition					1306:1316	a unique composition	1297:1316	a unique composition	1297:1316	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	5	35	theme	monosaccharide	692:705	arg1	fructose					707:714	the monosaccharide fructose	688:714	the monosaccharide fructose	688:714	The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env.					
32102878	9	36	theme	synthetic	1523:1531	arg1	simulant					1533:1540	a synthetic simulant	1521:1540	a synthetic simulant that we formulated	1521:1559	Here, we describe HIV-1 infection and inhibition in whole human seminal plasma and a synthetic simulant that we formulated.					
32102878	3	37	theme	unstable	323:330	arg1	nature					332:337	the unstable nature	319:337	the unstable nature of human seminal plasma	319:361	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	3	38	theme	in	432:433	arg1	infection					447:455	in vitro virus infection	432:455	in vitro virus infection	432:455	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	7	39	theme	antibodies.IMPORTANCE	1181:1201	arg1	secretions					1214:1223	mucosal antibodies.IMPORTANCE Biological secretions	1173:1223	mucosal antibodies.IMPORTANCE Biological secretions	1173:1223	Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies.IMPORTANCE Biological secretions allow viruses to spread between individuals.					
32102878	11	40	theme	HIV-1	1856:1860	arg1	transmission					1840:1851	the sexual transmission	1829:1851	the sexual transmission of HIV-1	1829:1860	This effect of semen fructose likely reduces the efficacy of such inhibitors to prevent the sexual transmission of HIV-1.					
32102878	3	41	theme	seminal	348:354	arg1	plasma					356:361	human seminal plasma	342:361	human seminal plasma	342:361	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	4	42	theme	whole	502:506	arg1	semen					508:512	whole semen	502:512	whole semen	502:512	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	12	43	dep	in	1990:1991	arg1	vitro					1993:1997	vitro	1993:1997	vitro	1993:1997	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	8	44	theme	infectivity	1371:1381	arg1	potential					1383:1391	the infectivity potential	1367:1391	the infectivity potential of the virus	1367:1404	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	10	45	theme	class	1654:1658	arg1	activity					1623:1630	the activity	1619:1630	the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1	1619:1738	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	7	46	theme	antiviral	1146:1154	arg1	microbicides					1156:1167	antiviral microbicides	1146:1167	antiviral microbicides	1146:1167	Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies.IMPORTANCE Biological secretions allow viruses to spread between individuals.					
32102878	0	47	theme	Fructose	20:27	arg1	Content					9:15	The High Content	0:15	The High Content of Fructose in Human Semen	0:42	The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans.					
32102878	3	48	theme	toxic	371:375	arg1	effects					377:383	its toxic effects	367:383	its toxic effects on cells in culture	367:403	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	8	49	theme	virus	1400:1404	arg1	inhibition					1410:1419	inhibition	1410:1419	inhibition of this process	1410:1435	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	8	49	theme	virus	1400:1404	arg1	potential					1383:1391	the infectivity potential	1367:1391	the infectivity potential of the virus	1367:1404	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	0	50	theme	Human	32:36	arg1	Semen					38:42	Human Semen	32:42	Human Semen	32:42	The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans.					
32102878	2	51	theme	Initial	203:209	arg1	cell					244:247	cell attachment and entry	244:268	cell attachment and entry	244:268	Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen.					
32102878	2	51	theme	Initial	203:209	arg1	steps					211:215	Initial steps	203:215	Initial steps	203:215	Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen.					
32102878	10	52	theme	antiviral	1663:1671	arg1	agents					1673:1678	antiviral agents	1663:1678	antiviral agents that target mannose sugars on the envelope protein of HIV-1	1663:1738	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	7	53	theme	mucosal	1173:1179	arg1	secretions					1214:1223	mucosal antibodies.IMPORTANCE Biological secretions	1173:1223	mucosal antibodies.IMPORTANCE Biological secretions	1173:1223	Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies.IMPORTANCE Biological secretions allow viruses to spread between individuals.					
32102878	3	54	dep	in	432:433	arg1	vitro					435:439	vitro	435:439	vitro	435:439	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	4	55	gly	glycoproteins	615:627	arg1	Envs					630:633	Envs	630:633	Envs	630:633	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	4	55	gly	glycoproteins	615:627	arg1	glycoproteins					615:627	the envelope glycoproteins	602:627	the envelope glycoproteins (Envs) of HIV-1	602:643	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	10	56	theme	mannose	1692:1698	arg1	sugars					1700:1705	mannose sugars	1692:1705	mannose sugars on the envelope protein of HIV-1	1692:1738	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	5	57	from	concentration	671:683	arg1	semen					719:723	semen	719:723	semen	719:723	The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env.					
32102878	6	58	theme	human	890:894	arg1	plasma					904:909	whole human seminal plasma	884:909	whole human seminal plasma	884:909	Infection and inhibition in whole human seminal plasma are accurately mimicked by a stable synthetic simulant of seminal fluid that we formulated.					
32102878	8	59	contain	has	1293:1295	arg1	type					1275:1278	Each type	1270:1278	Each type of secretion	1270:1291	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	8	59	contain	has	1293:1295	arg2	composition					1306:1316	a unique composition	1297:1316	a unique composition	1297:1316	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	7	60	theme	secretions	1080:1089	arg1	content					1058:1064	the protein content	1046:1064	the protein content of biological secretions	1046:1089	Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies.IMPORTANCE Biological secretions allow viruses to spread between individuals.					
32102878	10	61	theme	envelope	1714:1721	arg1	protein					1723:1729	the envelope protein	1710:1729	the envelope protein of HIV-1	1710:1738	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	2	62	dep	cell	244:247	arg1	attachment					249:258	attachment	249:258	attachment	249:258	Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen.					
32102878	2	62	dep	cell	244:247	arg1	entry					264:268	entry	264:268	entry	264:268	Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen.					
32102878	10	63	theme	sugar	1585:1589	arg1	fructose					1591:1598	the sugar fructose	1581:1598	the sugar fructose in semen	1581:1607	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	7	64	theme	protein	1050:1056	arg1	content					1058:1064	the protein content	1046:1064	the protein content of biological secretions	1046:1089	Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies.IMPORTANCE Biological secretions allow viruses to spread between individuals.					
32102878	2	65	theme	semen	303:307	arg1	presence					291:298	the presence	287:298	the presence of semen	287:307	Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen.					
32102878	1	66	theme	various	175:181	arg1	viruses					194:200	various pathogenic viruses	175:200	various pathogenic viruses	175:200	Semen is the primary transmission vehicle for various pathogenic viruses.					
32102878	12	67	theme	vaccine-elicited	1936:1951	arg1	antibodies					1953:1962	vaccine-elicited antibodies	1936:1962	vaccine-elicited antibodies	1936:1962	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	5	68	theme	ligands	808:814	arg1	binding					797:803	the binding	793:803	the binding of ligands to α1,2-linked mannose residues on Env	793:853	The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env.					
32102878	6	69	theme	stable	940:945	arg1	simulant					957:964	a stable synthetic simulant	938:964	a stable synthetic simulant of seminal fluid that we formulated	938:1000	Infection and inhibition in whole human seminal plasma are accurately mimicked by a stable synthetic simulant of seminal fluid that we formulated.					
32102878	12	70	theme	HIV-1	2074:2078	arg1	infection					2080:2088	HIV-1 infection	2074:2088	HIV-1 infection	2074:2088	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	11	71	theme	semen	1756:1760	arg1	fructose					1762:1769	semen fructose	1756:1769	semen fructose	1756:1769	This effect of semen fructose likely reduces the efficacy of such inhibitors to prevent the sexual transmission of HIV-1.					
32102878	3	72	from	nature	332:337	arg1	cells					388:392	cells	388:392	cells in culture	388:403	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	4	73	from	glycans	591:597	arg1	Envs					630:633	Envs	630:633	Envs	630:633	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	4	73	from	glycans	591:597	arg1	glycoproteins					615:627	the envelope glycoproteins	602:627	the envelope glycoproteins (Envs) of HIV-1	602:643	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	9	74	theme	HIV-1	1456:1460	arg1	infection					1462:1470	HIV-1 infection	1456:1470	HIV-1 infection	1456:1470	Here, we describe HIV-1 infection and inhibition in whole human seminal plasma and a synthetic simulant that we formulated.					
32102878	8	75	theme	secretion	1283:1291	arg1	type					1275:1278	Each type	1270:1278	Each type of secretion	1270:1291	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	2	76	theme	transmission	220:231	arg1	cell					244:247	cell attachment and entry	244:268	cell attachment and entry	244:268	Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen.					
32102878	2	76	theme	transmission	220:231	arg1	steps					211:215	Initial steps	203:215	Initial steps	203:215	Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen.					
32102878	12	77	theme	microbicides	1919:1930	arg1	evaluation					1905:1914	the preclinical evaluation	1889:1914	the preclinical evaluation of microbicides and vaccine-elicited antibodies	1889:1962	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	12	78	from	effects	2054:2060	arg1	inhibition					2094:2103	inhibition	2094:2103	inhibition	2094:2103	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	12	78	from	effects	2054:2060	arg1	infection					2080:2088	HIV-1 infection	2074:2088	HIV-1 infection	2074:2088	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	12	79	theme	semen	2065:2069	arg1	effects					2054:2060	the effects	2050:2060	the effects of semen on HIV-1 infection and inhibition	2050:2103	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	11	80	theme	such	1802:1805	arg1	inhibitors					1807:1816	such inhibitors	1802:1816	such inhibitors	1802:1816	This effect of semen fructose likely reduces the efficacy of such inhibitors to prevent the sexual transmission of HIV-1.					
32102878	9	81	theme	human	1496:1500	arg1	plasma					1510:1515	whole human seminal plasma	1490:1515	whole human seminal plasma	1490:1515	Here, we describe HIV-1 infection and inhibition in whole human seminal plasma and a synthetic simulant that we formulated.					
32102878	8	82	theme	proteins	1321:1328	arg1	composition					1306:1316	a unique composition	1297:1316	a unique composition	1297:1316	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	0	83	theme	Potent	77:82	arg1	Antivirals					84:93	Broad and Potent Antivirals	67:93	Broad and Potent Antivirals That Target High-Mannose Glycans	67:126	The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans.					
32102878	3	84	from	effects	377:383	arg1	cells					388:392	cells	388:392	cells in culture	388:403	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	5	85	link	α1,2-linked	819:829	arg1	residues					839:846	α1,2-linked mannose residues	819:846	α1,2-linked mannose residues on Env	819:853	The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env.					
32102878	1	86	theme	primary	142:148	arg1	vehicle					163:169	the primary transmission vehicle	138:169	the primary transmission vehicle for various pathogenic viruses	138:200	Semen is the primary transmission vehicle for various pathogenic viruses.					
32102878	1	86	theme	primary	142:148	arg1	Semen					129:133	Semen	129:133	Semen	129:133	Semen is the primary transmission vehicle for various pathogenic viruses.					
32102878	0	87	theme	High-Mannose	107:118	arg1	Glycans					120:126	Target High-Mannose Glycans	100:126	Target High-Mannose Glycans	100:126	The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans.					
32102878	8	88	theme	salts	1331:1335	arg1	composition					1306:1316	a unique composition	1297:1316	a unique composition	1297:1316	Each type of secretion has a unique composition of proteins, salts, and sugars, which can affect the infectivity potential of the virus and inhibition of this process.					
32102878	7	89	theme	Biological	1203:1212	arg1	secretions					1214:1223	mucosal antibodies.IMPORTANCE Biological secretions	1173:1223	mucosal antibodies.IMPORTANCE Biological secretions	1173:1223	Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies.IMPORTANCE Biological secretions allow viruses to spread between individuals.					
32102878	3	90	from	cells	388:392	arg1	culture					397:403	culture	397:403	culture	397:403	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	12	91	theme	preclinical	1893:1903	arg1	evaluation					1905:1914	the preclinical evaluation	1889:1914	the preclinical evaluation of microbicides and vaccine-elicited antibodies	1889:1962	Our findings suggest that the preclinical evaluation of microbicides and vaccine-elicited antibodies will be improved by their in vitro assessment in synthetic formulations that simulate the effects of semen on HIV-1 infection and inhibition.					
32102878	6	92	theme	seminal	969:975	arg1	fluid					977:981	seminal fluid	969:981	seminal fluid that we formulated	969:1000	Infection and inhibition in whole human seminal plasma are accurately mimicked by a stable synthetic simulant of seminal fluid that we formulated.					
32102878	3	93	theme	human	342:346	arg1	plasma					356:361	human seminal plasma	342:361	human seminal plasma	342:361	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	11	94	theme	inhibitors	1807:1816	arg1	efficacy					1790:1797	the efficacy	1786:1797	the efficacy of such inhibitors to prevent the sexual transmission of HIV-1	1786:1860	This effect of semen fructose likely reduces the efficacy of such inhibitors to prevent the sexual transmission of HIV-1.					
32102878	0	95	theme	Target	100:105	arg1	Glycans					120:126	Target High-Mannose Glycans	100:126	Target High-Mannose Glycans	100:126	The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans.					
32102878	5	96	theme	fructose	707:714	arg1	concentration					671:683	The extraordinarily high concentration	646:683	The extraordinarily high concentration of the monosaccharide fructose in semen	646:723	The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env.					
32102878	0	97	theme	High	4:7	arg1	Content					9:15	The High Content	0:15	The High Content of Fructose in Human Semen	0:42	The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans.					
32102878	3	98	theme	plasma	356:361	arg1	nature					332:337	the unstable nature	319:337	the unstable nature of human seminal plasma	319:361	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	3	98	theme	plasma	356:361	arg1	effects					377:383	its toxic effects	367:383	its toxic effects on cells in culture	367:403	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	10	99	theme	potent	1647:1652	arg1	class					1654:1658	a broad and potent class	1635:1658	a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1	1635:1738	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	5	100	theme	α1,2-linked	819:829	arg1	residues					839:846	α1,2-linked mannose residues	819:846	α1,2-linked mannose residues on Env	819:853	The extraordinarily high concentration of the monosaccharide fructose in semen contributes significantly to the effect by competitively inhibiting the binding of ligands to α1,2-linked mannose residues on Env.					
32102878	10	101	theme	broad	1637:1641	arg1	class					1654:1658	a broad and potent class	1635:1658	a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1	1635:1738	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	10	102	theme	agents	1673:1678	arg1	class					1654:1658	a broad and potent class	1635:1658	a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1	1635:1738	We discovered that the sugar fructose in semen decreases the activity of a broad and potent class of antiviral agents that target mannose sugars on the envelope protein of HIV-1.					
32102878	4	103	theme	microbicides	566:577	arg1	potency					540:546	the potency	536:546	the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1	536:643	We found that whole semen significantly reduces the potency of antibodies and microbicides that target glycans on the envelope glycoproteins (Envs) of HIV-1.					
32102878	3	104	theme	virus	441:445	arg1	infection					447:455	in vitro virus infection	432:455	in vitro virus infection	432:455	However, the unstable nature of human seminal plasma and its toxic effects on cells in culture limit the ability to study in vitro virus infection and inhibition in this medium.					
32102878	7	105	theme	microbicides	1156:1167	arg1	potency					1135:1141	the potency	1131:1141	the potency of antiviral microbicides	1131:1167	Our findings indicate that, in addition to the protein content of biological secretions, their small-solute composition impacts the potency of antiviral microbicides and mucosal antibodies.IMPORTANCE Biological secretions allow viruses to spread between individuals.					
33234262	0	0	theme	cancer	95:100	arg1	inhibition					102:111	colorectal cancer inhibition	84:111	colorectal cancer inhibition	84:111	Application of a polyelectrolyte complex based on biocompatible polysaccharides for colorectal cancer inhibition.					
33234262	8	1	theme	Brunauer-Emmett-Teller	1113:1134	arg1	isotherm					1142:1149	The Brunauer-Emmett-Teller (BET) isotherm	1109:1149	The Brunauer-Emmett-Teller (BET) isotherm	1109:1149	The Brunauer-Emmett-Teller (BET) isotherm and pseudo-second-order kinetic fitted well to the experimental data.					
33234262	2	2	theme	/chondroitin	353:364	arg1	CS					376:377	CS	376:377	CS	376:377	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	2	theme	/chondroitin	353:364	arg1	sulphate					366:373	chitosan (CHT)/chondroitin sulphate	339:373	chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc)	339:542	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	11	3	theme	first	1514:1518	arg1	time					1520:1523	the first time	1510:1523	the first time	1510:1523	For the first time, we associate PS-b-PAA/AlClPc with a hydrophilic and cytocompatible polysaccharide matrix.					
33234262	0	4	theme	colorectal	84:93	arg1	inhibition					102:111	colorectal cancer inhibition	84:111	colorectal cancer inhibition	84:111	Application of a polyelectrolyte complex based on biocompatible polysaccharides for colorectal cancer inhibition.					
33234262	7	5	theme	only	1096:1099	arg1	15 min					1101:1106	only 15 min	1096:1106	only 15 min	1096:1106	The encapsulation efficiency reached 95% at 190 μg mL-1 AlClPc after only 15 min.					
33234262	4	6	from	NPs	726:728	arg1	surface					741:747	the PEC surface	733:747	the PEC surface	733:747	The PS-b-PAA NPs on the PEC surface was confirmed by scanning electron microscopy (SEM).					
33234262	1	7	theme	drug	184:187	arg1	systems					198:204	drug delivery systems	184:204	drug delivery systems	184:204	Strategies for incorporating water-insoluble photosensitisers (PS) in drug delivery systems have been extensively studied.					
33234262	12	8	theme	photodynamic	1693:1704	arg1	therapy					1706:1712	photodynamic therapy	1693:1712	photodynamic therapy	1693:1712	We suggest the use of these materials in strategies to treat cancer by using photodynamic therapy.					
33234262	10	9	theme	epithelial	1423:1432	arg1	cells					1434:1438	kidney epithelial cells	1416:1438	kidney epithelial cells	1416:1438	The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells).					
33234262	10	9	theme	epithelial	1423:1432	arg1	VERO					1410:1413	healthy VERO	1402:1413	healthy VERO (kidney epithelial cells)	1402:1439	The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells).					
33234262	2	10	theme	chloroaluminum	505:518	arg1	AlClPc					536:541	AlClPc	536:541	AlClPc	536:541	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	10	theme	chloroaluminum	505:518	arg1	phthalocyanine					520:533	chloroaluminum phthalocyanine	505:533	chloroaluminum phthalocyanine (AlClPc)	505:542	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	9	11	theme	AlClPc	1271:1276	arg1	bioavailability					1278:1292	the AlClPc bioavailability	1267:1292	the AlClPc bioavailability	1267:1292	The PS-b-PAA NPs on the PEC surfaces increase the AlClPc bioavailability and the PEC structure stabilizes the PS-b-PAA/AlClPc nanostructures.					
33234262	7	12	theme	190 μg mL-1	1071:1081	arg1	AlClPc					1083:1088	190 μg mL-1 AlClPc	1071:1088	190 μg mL-1 AlClPc	1071:1088	The encapsulation efficiency reached 95% at 190 μg mL-1 AlClPc after only 15 min.					
33234262	2	13	theme	sorption	301:308	arg1	studies					310:316	drug sorption studies	296:316	drug sorption studies	296:316	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	6	14	theme	equilibrium	938:948	arg1	studies					950:956	Kinetic and equilibrium studies	926:956	studies	950:956	Kinetic and equilibrium studies investigate the sorption capacity of the PEC/PS-b-PAA toward AlClPc.					
33234262	3	15	theme	scanning	677:684	arg1	SEM					707:709	SEM	707:709	SEM	707:709	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	3	15	theme	scanning	677:684	arg1	microscopy					695:704	scanning electron microscopy	677:704	scanning electron microscopy (SEM)	677:710	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	9	16	from	NPs	1234:1236	arg1	surfaces					1249:1256	the PEC surfaces	1241:1256	the PEC surfaces	1241:1256	The PS-b-PAA NPs on the PEC surfaces increase the AlClPc bioavailability and the PEC structure stabilizes the PS-b-PAA/AlClPc nanostructures.					
33234262	2	17	theme	drug	296:299	arg1	studies					310:316	drug sorption studies	296:316	drug sorption studies	296:316	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	3	18	theme	electron	686:693	arg1	SEM					707:709	SEM	707:709	SEM	707:709	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	3	18	theme	electron	686:693	arg1	microscopy					695:704	scanning electron microscopy	677:704	scanning electron microscopy (SEM)	677:710	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	10	19	theme	cancerous	1475:1483	arg1	HT-29 cells					1492:1502	HT-29 cells	1492:1502	HT-29 cells	1492:1502	The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells).					
33234262	10	19	theme	cancerous	1475:1483	arg1	cells					1485:1489	colorectal cancerous cells	1464:1489	colorectal cancerous cells (HT-29 cells)	1464:1503	The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells).					
33234262	1	20	theme	delivery	189:196	arg1	systems					198:204	drug delivery systems	184:204	drug delivery systems	184:204	Strategies for incorporating water-insoluble photosensitisers (PS) in drug delivery systems have been extensively studied.					
33234262	4	21	theme	electron	775:782	arg1	SEM					796:798	SEM	796:798	SEM	796:798	The PS-b-PAA NPs on the PEC surface was confirmed by scanning electron microscopy (SEM).					
33234262	4	21	theme	electron	775:782	arg1	microscopy					784:793	scanning electron microscopy	766:793	scanning electron microscopy (SEM)	766:799	The PS-b-PAA NPs on the PEC surface was confirmed by scanning electron microscopy (SEM).					
33234262	1	22	from	photosensitisers	159:174	arg1	systems					198:204	drug delivery systems	184:204	drug delivery systems	184:204	Strategies for incorporating water-insoluble photosensitisers (PS) in drug delivery systems have been extensively studied.					
33234262	4	23	theme	PS-b-PAA	717:724	arg1	NPs					726:728	The PS-b-PAA NPs	713:728	The PS-b-PAA NPs on the PEC surface	713:747	The PS-b-PAA NPs on the PEC surface was confirmed by scanning electron microscopy (SEM).					
33234262	2	24	theme	polyelectrolyte	380:394	arg1	PECs					407:410	PECs	407:410	PECs	407:410	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	24	theme	polyelectrolyte	380:394	arg1	complexes					396:404	chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes	339:404	chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc)	339:542	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	25	theme	acrylic	448:454	arg1	NPs					488:490	NPs	488:490	NPs	488:490	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	25	theme	acrylic	448:454	arg1	nanoparticles					473:485	polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles	425:485	polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc)	425:542	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	9	26	theme	PEC	1302:1304	arg1	structure					1306:1314	the PEC structure	1298:1314	the PEC structure	1298:1314	The PS-b-PAA NPs on the PEC surfaces increase the AlClPc bioavailability and the PEC structure stabilizes the PS-b-PAA/AlClPc nanostructures.					
33234262	10	27	theme	kidney	1416:1421	arg1	cells					1434:1438	kidney epithelial cells	1416:1438	kidney epithelial cells	1416:1438	The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells).					
33234262	10	27	theme	kidney	1416:1421	arg1	VERO					1410:1413	healthy VERO	1402:1413	healthy VERO (kidney epithelial cells)	1402:1439	The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells).					
33234262	6	28	theme	Kinetic	926:932	arg1	studies					950:956	Kinetic and equilibrium studies	926:956	studies	950:956	Kinetic and equilibrium studies investigate the sorption capacity of the PEC/PS-b-PAA toward AlClPc.					
33234262	11	29	theme	hydrophilic	1562:1572	arg1	matrix					1608:1613	a hydrophilic and cytocompatible polysaccharide matrix	1560:1613	a hydrophilic and cytocompatible polysaccharide matrix	1560:1613	For the first time, we associate PS-b-PAA/AlClPc with a hydrophilic and cytocompatible polysaccharide matrix.					
33234262	2	30	theme	polystyrene-block-poly	425:446	arg1	NPs					488:490	NPs	488:490	NPs	488:490	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	30	theme	polystyrene-block-poly	425:446	arg1	nanoparticles					473:485	polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles	425:485	polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc)	425:542	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	7	31	theme	encapsulation	1031:1043	arg1	efficiency					1045:1054	The encapsulation efficiency	1027:1054	The encapsulation efficiency	1027:1054	The encapsulation efficiency reached 95% at 190 μg mL-1 AlClPc after only 15 min.					
33234262	10	32	theme	healthy	1402:1408	arg1	cells					1434:1438	kidney epithelial cells	1416:1438	kidney epithelial cells	1416:1438	The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells).					
33234262	10	32	theme	healthy	1402:1408	arg1	VERO					1410:1413	healthy VERO	1402:1413	healthy VERO (kidney epithelial cells)	1402:1439	The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells).					
33234262	9	33	theme	PS-b-PAA	1225:1232	arg1	NPs					1234:1236	The PS-b-PAA NPs	1221:1236	The PS-b-PAA NPs on the PEC surfaces	1221:1256	The PS-b-PAA NPs on the PEC surfaces increase the AlClPc bioavailability and the PEC structure stabilizes the PS-b-PAA/AlClPc nanostructures.					
33234262	12	34	from	use	1631:1633	arg1	strategies					1657:1666	strategies	1657:1666	strategies to treat cancer by using photodynamic therapy	1657:1712	We suggest the use of these materials in strategies to treat cancer by using photodynamic therapy.					
33234262	12	35	theme	materials	1644:1652	arg1	use					1631:1633	the use	1627:1633	the use of these materials in strategies to treat cancer by using photodynamic therapy	1627:1712	We suggest the use of these materials in strategies to treat cancer by using photodynamic therapy.					
33234262	2	36	theme	PS-b-PAA	463:470	arg1	NPs					488:490	NPs	488:490	NPs	488:490	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	36	theme	PS-b-PAA	463:470	arg1	nanoparticles					473:485	polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles	425:485	polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc)	425:542	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	9	37	theme	PS-b-PAA/AlClPc	1331:1345	arg1	nanostructures					1347:1360	the PS-b-PAA/AlClPc nanostructures	1327:1360	the PS-b-PAA/AlClPc nanostructures	1327:1360	The PS-b-PAA NPs on the PEC surfaces increase the AlClPc bioavailability and the PEC structure stabilizes the PS-b-PAA/AlClPc nanostructures.					
33234262	3	38	theme	infrared	576:583	arg1	FTIR					599:602	FTIR	599:602	FTIR	599:602	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	3	38	theme	infrared	576:583	arg1	spectroscopy					585:596	infrared spectroscopy	576:596	infrared spectroscopy (FTIR)	576:603	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	6	39	theme	PEC/PS-b-PAA	999:1010	arg1	capacity					983:990	the sorption capacity	970:990	the sorption capacity of the PEC/PS-b-PAA toward AlClPc	970:1024	Kinetic and equilibrium studies investigate the sorption capacity of the PEC/PS-b-PAA toward AlClPc.					
33234262	0	40	theme	complex	33:39	arg1	Application					0:10	Application	0:10	Application of a polyelectrolyte complex	0:39	Application of a polyelectrolyte complex based on biocompatible polysaccharides for colorectal cancer inhibition.					
33234262	3	41	theme	differential	606:617	arg1	calorimetric					628:639	differential scanning calorimetric	606:639	differential scanning calorimetric (DSC)	606:645	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	3	41	theme	differential	606:617	arg1	DSC					642:644	DSC	642:644	DSC	642:644	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	0	42	theme	polyelectrolyte	17:31	arg1	complex					33:39	a polyelectrolyte complex	15:39	a polyelectrolyte complex	15:39	Application of a polyelectrolyte complex based on biocompatible polysaccharides for colorectal cancer inhibition.					
33234262	11	43	theme	polysaccharide	1593:1606	arg1	matrix					1608:1613	a hydrophilic and cytocompatible polysaccharide matrix	1560:1613	a hydrophilic and cytocompatible polysaccharide matrix	1560:1613	For the first time, we associate PS-b-PAA/AlClPc with a hydrophilic and cytocompatible polysaccharide matrix.					
33234262	0	44	theme	biocompatible	50:62	arg1	polysaccharides					64:78	biocompatible polysaccharides	50:78	biocompatible polysaccharides for colorectal cancer inhibition	50:111	Application of a polyelectrolyte complex based on biocompatible polysaccharides for colorectal cancer inhibition.					
33234262	8	45	theme	BET	1137:1139	arg1	isotherm					1142:1149	The Brunauer-Emmett-Teller (BET) isotherm	1109:1149	The Brunauer-Emmett-Teller (BET) isotherm	1109:1149	The Brunauer-Emmett-Teller (BET) isotherm and pseudo-second-order kinetic fitted well to the experimental data.					
33234262	3	46	theme	scanning	619:626	arg1	calorimetric					628:639	differential scanning calorimetric	606:639	differential scanning calorimetric (DSC)	606:645	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	3	46	theme	scanning	619:626	arg1	DSC					642:644	DSC	642:644	DSC	642:644	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	4	47	theme	scanning	766:773	arg1	SEM					796:798	SEM	796:798	SEM	796:798	The PS-b-PAA NPs on the PEC surface was confirmed by scanning electron microscopy (SEM).					
33234262	4	47	theme	scanning	766:773	arg1	microscopy					784:793	scanning electron microscopy	766:793	scanning electron microscopy (SEM)	766:799	The PS-b-PAA NPs on the PEC surface was confirmed by scanning electron microscopy (SEM).					
33234262	5	48	contain	containing	864:873	arg2	PS-b-PAA					875:882	PS-b-PAA	875:882	PS-b-PAA	875:882	Additionally, optical images distinguished the PEC structures containing PS-b-PAA or PS-b-PAA/AlClPc from the unloaded PEC.					
33234262	5	48	contain	containing	864:873	arg1	structures					853:862	the PEC structures	845:862	the PEC structures containing PS-b-PAA or PS-b-PAA/AlClPc	845:901	Additionally, optical images distinguished the PEC structures containing PS-b-PAA or PS-b-PAA/AlClPc from the unloaded PEC.					
33234262	5	48	contain	containing	864:873	arg2	PS-b-PAA/AlClPc					887:901	PS-b-PAA/AlClPc	887:901	PS-b-PAA/AlClPc	887:901	Additionally, optical images distinguished the PEC structures containing PS-b-PAA or PS-b-PAA/AlClPc from the unloaded PEC.					
33234262	2	49	theme	CHT	349:351	arg1	CS					376:377	CS	376:377	CS	376:377	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	49	theme	CHT	349:351	arg1	sulphate					366:373	chitosan (CHT)/chondroitin sulphate	339:373	chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc)	339:542	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	50	theme	acid	456:459	arg1	NPs					488:490	NPs	488:490	NPs	488:490	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	50	theme	acid	456:459	arg1	nanoparticles					473:485	polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles	425:485	polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc)	425:542	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	51	theme	complexes	396:404	arg1	characterisation					278:293	characterisation	278:293	characterisation	278:293	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	51	theme	complexes	396:404	arg1	formation					267:275	the formation	263:275	the formation	263:275	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	51	theme	complexes	396:404	arg1	studies					310:316	drug sorption studies	296:316	drug sorption studies	296:316	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	51	theme	complexes	396:404	arg1	cytotoxicity					323:334	cytotoxicity	323:334	cytotoxicity	323:334	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	10	52	theme	colorectal	1464:1473	arg1	HT-29 cells					1492:1502	HT-29 cells	1492:1502	HT-29 cells	1492:1502	The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells).					
33234262	10	52	theme	colorectal	1464:1473	arg1	cells					1485:1489	colorectal cancerous cells	1464:1489	colorectal cancerous cells (HT-29 cells)	1464:1503	The materials were cytocompatible upon healthy VERO (kidney epithelial cells), and cytotoxic against colorectal cancerous cells (HT-29 cells).					
33234262	1	53	theme	water-insoluble	143:157	arg1	photosensitisers					159:174	water-insoluble photosensitisers	143:174	water-insoluble photosensitisers (PS) in drug delivery systems	143:204	Strategies for incorporating water-insoluble photosensitisers (PS) in drug delivery systems have been extensively studied.					
33234262	1	53	theme	water-insoluble	143:157	arg1	PS					177:178	PS	177:178	PS	177:178	Strategies for incorporating water-insoluble photosensitisers (PS) in drug delivery systems have been extensively studied.					
33234262	3	54	theme	X-ray	648:652	arg1	XRD					667:669	XRD	667:669	XRD	667:669	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	3	54	theme	X-ray	648:652	arg1	diffraction					654:664	X-ray diffraction	648:664	X-ray diffraction (XRD)	648:670	The PECs were characterised by infrared spectroscopy (FTIR), differential scanning calorimetric (DSC), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
33234262	11	55	theme	cytocompatible	1578:1591	arg1	matrix					1608:1613	a hydrophilic and cytocompatible polysaccharide matrix	1560:1613	a hydrophilic and cytocompatible polysaccharide matrix	1560:1613	For the first time, we associate PS-b-PAA/AlClPc with a hydrophilic and cytocompatible polysaccharide matrix.					
33234262	2	56	theme	chitosan	339:346	arg1	CS					376:377	CS	376:377	CS	376:377	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	56	theme	chitosan	339:346	arg1	sulphate					366:373	chitosan (CHT)/chondroitin sulphate	339:373	chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc)	339:542	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	5	57	theme	PEC	849:851	arg1	structures					853:862	the PEC structures	845:862	the PEC structures containing PS-b-PAA or PS-b-PAA/AlClPc	845:901	Additionally, optical images distinguished the PEC structures containing PS-b-PAA or PS-b-PAA/AlClPc from the unloaded PEC.					
33234262	5	58	theme	unloaded	912:919	arg1	PEC					921:923	the unloaded PEC	908:923	the unloaded PEC	908:923	Additionally, optical images distinguished the PEC structures containing PS-b-PAA or PS-b-PAA/AlClPc from the unloaded PEC.					
33234262	9	59	theme	PEC	1245:1247	arg1	surfaces					1249:1256	the PEC surfaces	1241:1256	the PEC surfaces	1241:1256	The PS-b-PAA NPs on the PEC surfaces increase the AlClPc bioavailability and the PEC structure stabilizes the PS-b-PAA/AlClPc nanostructures.					
33234262	5	60	theme	optical	816:822	arg1	images					824:829	optical images	816:829	optical images	816:829	Additionally, optical images distinguished the PEC structures containing PS-b-PAA or PS-b-PAA/AlClPc from the unloaded PEC.					
33234262	6	61	theme	sorption	974:981	arg1	capacity					983:990	the sorption capacity	970:990	the sorption capacity of the PEC/PS-b-PAA toward AlClPc	970:1024	Kinetic and equilibrium studies investigate the sorption capacity of the PEC/PS-b-PAA toward AlClPc.					
33234262	8	62	theme	experimental	1202:1213	arg1	data					1215:1218	the experimental data	1198:1218	the experimental data	1198:1218	The Brunauer-Emmett-Teller (BET) isotherm and pseudo-second-order kinetic fitted well to the experimental data.					
33234262	4	63	theme	PEC	737:739	arg1	surface					741:747	the PEC surface	733:747	the PEC surface	733:747	The PS-b-PAA NPs on the PEC surface was confirmed by scanning electron microscopy (SEM).					
33234262	12	64	dep	strategies	1657:1666	arg1	treat					1671:1675	treat	1671:1675	to treat cancer by using photodynamic therapy	1668:1712	We suggest the use of these materials in strategies to treat cancer by using photodynamic therapy.					
33234262	2	65	theme	sulphate	366:373	arg1	PECs					407:410	PECs	407:410	PECs	407:410	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
33234262	2	65	theme	sulphate	366:373	arg1	complexes					396:404	chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes	339:404	chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc)	339:542	In this work, we evaluate the formation, characterisation, drug sorption studies, and cytotoxicity of chitosan (CHT)/chondroitin sulphate (CS) polyelectrolyte complexes (PECs) coated with polystyrene-block-poly(acrylic acid) (PS-b-PAA) nanoparticles (NPs) loaded with chloroaluminum phthalocyanine (AlClPc).					
34361549	7	0	theme	2,4,6-trinitrate	1417:1432	arg1	phenyl					1434:1439	2,4,6-trinitrate phenyl	1417:1439	2,4,6-trinitrate phenyl	1417:1439	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	6	1	from	effects	1179:1185	arg1	α-glucosidase					1190:1202	α-glucosidase	1190:1202	α-glucosidase	1190:1202	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	7	2	theme	2,2-diphenyl-1-	1401:1415	arg1	ability					1471:1477	2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability	1401:1477	2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL)	1401:1566	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	2	theme	2,2-diphenyl-1-	1401:1415	arg1	values					1485:1490	IC50 values	1480:1490	IC50 values	1480:1490	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	3	theme	±	1686:1686	arg1	mg/mL					1694:1698	1.744 ± 0.080 mg/mL	1680:1698	1.744 ± 0.080 mg/mL	1680:1698	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	3	4	theme	weight	492:497	arg1	distributions					499:511	Low molecular weight distributions	478:511	Low molecular weight distributions	478:511	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	7	5	theme	vitamin	1365:1371	arg1	C					1373:1373	vitamin C	1365:1373	vitamin C	1365:1373	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	3	6	theme	hot	576:578	arg1	LLP-H					592:596	LLP-H	592:596	LLP-H	592:596	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	3	6	theme	hot	576:578	arg1	drying					584:589	hot air drying	576:589	hot air drying (LLP-H)	576:597	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	1	7	theme	techniques	170:179	arg1	influence					145:153	the influence	141:153	the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs)	141:267	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	7	8	theme	0.053	1651:1655	arg1	±					1649:1649	±	1649:1649	±	1649:1649	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	9	theme	±	1350:1350	arg1	mg/mL					1358:1362	0.176 ± 0.004 mg/mL	1344:1362	0.176 ± 0.004 mg/mL	1344:1362	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	9	theme	±	1350:1350	arg1	LLP-H					1337:1341	LLP-H	1337:1341	LLP-H	1337:1341	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	10	theme	±	1553:1553	arg1	mg/mL					1561:1565	0.366 ± 0.010 mg/mL	1547:1565	0.366 ± 0.010 mg/mL	1547:1565	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	6	11	theme	inhibitory	1168:1177	arg1	effects					1179:1185	inhibitory effects	1168:1185	inhibitory effects on α-glucosidase	1168:1202	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	1	12	from	lotus	249:253	arg1	polysaccharides					228:242	polysaccharides	228:242	polysaccharides from lotus leaves (LLPs)	228:267	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	1	12	from	lotus	249:253	arg1	properties					214:223	the structural and biological properties	184:223	the structural and biological properties of polysaccharides from lotus leaves (LLPs)	184:267	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	8	13	theme	air	1864:1866	arg1	drying					1868:1873	hot air drying	1860:1873	hot air drying	1860:1873	These findings indicate that both hot air drying and microwave drying can be potential drying techniques for the pre-processing of lotus leaves for industrial applications.					
34361549	3	14	located	observed	518:525	arg2	distributions					499:511	Low molecular weight distributions	478:511	Low molecular weight distributions	478:511	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	3	14	located	observed	518:525	arg1	polysaccharides					530:544	polysaccharides	530:544	polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively	530:674	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	7	15	dep	values	1329:1334	arg1	mg/mL					1390:1394	0.043 ± 0.002 mg/mL	1376:1394	0.043 ± 0.002 mg/mL	1376:1394	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	15	dep	values	1329:1334	arg1	LLP-H					1337:1341	LLP-H	1337:1341	LLP-H	1337:1341	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	15	dep	values	1329:1334	arg1	mg/mL					1358:1362	0.176 ± 0.004 mg/mL	1344:1362	0.176 ± 0.004 mg/mL	1344:1362	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	15	dep	values	1329:1334	arg1	C					1373:1373	vitamin C	1365:1373	vitamin C	1365:1373	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	16	dep	values	1485:1490	arg1	hydroxytoluene					1531:1544	butylated hydroxytoluene	1521:1544	butylated hydroxytoluene	1521:1544	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	16	dep	values	1485:1490	arg1	LLP-H					1493:1497	LLP-H	1493:1497	LLP-H	1493:1497	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	16	dep	values	1485:1490	arg1	mg/mL					1514:1518	0.241 ± 0.007 mg/mL	1500:1518	0.241 ± 0.007 mg/mL	1500:1518	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	16	dep	values	1485:1490	arg1	mg/mL					1561:1565	0.366 ± 0.010 mg/mL	1547:1565	0.366 ± 0.010 mg/mL	1547:1565	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	6	17	theme	obvious	1064:1070	arg1	effects					1179:1185	inhibitory effects	1168:1185	inhibitory effects on α-glucosidase	1168:1202	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	6	17	theme	obvious	1064:1070	arg1	properties					1083:1092	obvious biological properties	1064:1092	obvious biological properties	1064:1092	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	6	17	theme	obvious	1064:1070	arg1	capacities					1126:1135	in vitro antioxidant capacities	1105:1135	in vitro antioxidant capacities	1105:1135	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	6	17	theme	obvious	1064:1070	arg1	activities					1152:1161	antiglycation activities	1138:1161	antiglycation activities	1138:1161	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	7	18	theme	butylated	1521:1529	arg1	hydroxytoluene					1531:1544	butylated hydroxytoluene	1521:1544	butylated hydroxytoluene	1521:1544	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	19	theme	IC50	1324:1327	arg1	ability					1315:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability	1229:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL)	1229:1395	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	19	theme	IC50	1324:1327	arg1	values					1329:1334	IC50 values	1324:1334	IC50 values	1324:1334	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	6	20	theme	antiglycation	1138:1150	arg1	activities					1152:1161	antiglycation activities	1138:1161	antiglycation activities	1138:1161	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	4	21	theme	high	681:684	arg1	contents					686:693	The high contents	677:693	The high contents of bound polyphenolics	677:716	The high contents of bound polyphenolics were measured in LLP-H and LLP-M, as well as polysaccharides obtained from lotus leaves by vacuum drying (LLP-V).					
34361549	6	22	from	activities	1152:1161	arg1	α-glucosidase					1190:1202	α-glucosidase	1190:1202	α-glucosidase	1190:1202	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	1	23	from	properties	214:223	arg1	lotus					249:253	lotus leaves	249:260	lotus leaves (LLPs)	249:267	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	1	23	from	properties	214:223	arg1	LLPs					263:266	LLPs	263:266	LLPs	263:266	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	5	24	theme	drying	968:973	arg1	technologies					975:986	drying technologies	968:986	drying technologies	968:986	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	1	25	theme	present	126:132	arg1	study					134:138	the present study	122:138	the present study	122:138	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	1	26	dep	lotus	249:253	arg1	leaves					255:260	leaves	255:260	leaves	255:260	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	8	27	theme	hot	1860:1862	arg1	drying					1868:1873	hot air drying	1860:1873	hot air drying	1860:1873	These findings indicate that both hot air drying and microwave drying can be potential drying techniques for the pre-processing of lotus leaves for industrial applications.					
34361549	7	28	theme	IC50	1623:1626	arg1	antiglycation					1608:1620	stronger antiglycation	1599:1620	stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL)	1599:1699	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	28	theme	IC50	1623:1626	arg1	values					1628:1633	IC50 values	1623:1633	IC50 values	1623:1633	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	29	theme	16.93	1800:1804	arg1	μg/mL					1806:1810	724.98 ± 16.93 μg/mL	1791:1810	724.98 ± 16.93 μg/mL	1791:1810	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	5	30	dep	transform	858:866	arg1	infrared					868:875	infrared	868:875	transform infrared	858:875	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	5	31	theme	basic	1009:1013	arg1	structures					1024:1033	their basic chemical structures	1003:1033	their basic chemical structures	1003:1033	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	6	32	theme	in	1105:1106	arg1	capacities					1126:1135	in vitro antioxidant capacities	1105:1135	in vitro antioxidant capacities	1105:1135	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	5	33	theme	magnetic	897:904	arg1	NMR					917:919	NMR	917:919	NMR	917:919	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	5	33	theme	magnetic	897:904	arg1	resonance					906:914	nuclear magnetic resonance	889:914	nuclear magnetic resonance (NMR) spectra of LLPs	889:936	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	7	34	dep	values	1747:1752	arg1	μg/mL					1806:1810	724.98 ± 16.93 μg/mL	1791:1810	724.98 ± 16.93 μg/mL	1791:1810	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	34	dep	values	1747:1752	arg1	acarbose					1781:1788	acarbose	1781:1788	acarbose	1781:1788	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	34	dep	values	1747:1752	arg1	μg/mL					1774:1778	1.90 ± 0.02 μg/mL	1762:1778	1.90 ± 0.02 μg/mL	1762:1778	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	34	dep	values	1747:1752	arg1	LLP-M					1755:1759	LLP-M	1755:1759	LLP-M	1755:1759	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	0	35	theme	Drying	100:105	arg1	Techniques					107:116	Drying Techniques	100:116	Drying Techniques	100:116	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.					
34361549	7	36	theme	scavenging	1304:1313	arg1	ability					1315:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability	1229:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL)	1229:1395	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	36	theme	scavenging	1304:1313	arg1	values					1329:1334	IC50 values	1324:1334	IC50 values	1324:1334	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	36	theme	scavenging	1304:1313	arg1	acid					1290:1293	3-ethylbenzothiazoline-6-sulfonic acid	1256:1293	3-ethylbenzothiazoline-6-sulfonic acid	1256:1293	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	2	37	theme	LLPs	432:435	arg1	monosaccharides					413:427	compositional monosaccharides	399:427	compositional monosaccharides of LLPs	399:435	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	2	38	theme	molecular	360:368	arg1	weights					370:376	molecular weights	360:376	molecular weights	360:376	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	0	39	theme	Structural	0:9	arg1	Properties					26:35	Structural and Biological Properties	0:35	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.	0:117	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.					
34361549	8	40	theme	potential	1903:1911	arg1	techniques					1920:1929	potential drying techniques	1903:1929	potential drying techniques for the pre-processing of lotus leaves for industrial applications	1903:1996	These findings indicate that both hot air drying and microwave drying can be potential drying techniques for the pre-processing of lotus leaves for industrial applications.					
34361549	2	41	theme	monosaccharides	413:427	arg1	weights					370:376	molecular weights	360:376	molecular weights	360:376	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	2	41	theme	monosaccharides	413:427	arg1	contents					321:328	contents	321:328	contents of basic chemical components	321:357	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	2	41	theme	monosaccharides	413:427	arg1	ratios					389:394	molar ratios	383:394	molar ratios	383:394	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	2	41	theme	monosaccharides	413:427	arg1	yields					313:318	the yields	309:318	the yields	309:318	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	7	42	dep	values	1628:1633	arg1	mg/mL					1657:1661	1.023 ± 0.053 mg/mL	1643:1661	1.023 ± 0.053 mg/mL	1643:1661	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	42	dep	values	1628:1633	arg1	LLP-M					1636:1640	LLP-M	1636:1640	LLP-M	1636:1640	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	42	dep	values	1628:1633	arg1	mg/mL					1694:1698	1.744 ± 0.080 mg/mL	1680:1698	1.744 ± 0.080 mg/mL	1680:1698	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	42	dep	values	1628:1633	arg1	aminoguanidine					1664:1677	aminoguanidine	1664:1677	aminoguanidine	1664:1677	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	4	43	theme	polyphenolics	704:716	arg1	contents					686:693	The high contents	677:693	The high contents of bound polyphenolics	677:716	The high contents of bound polyphenolics were measured in LLP-H and LLP-M, as well as polysaccharides obtained from lotus leaves by vacuum drying (LLP-V).					
34361549	0	44	theme	Biological	15:24	arg1	Properties					26:35	Structural and Biological Properties	0:35	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.	0:117	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.					
34361549	7	45	theme	3-ethylbenzothiazoline-6-sulfonic	1256:1288	arg1	ability					1315:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability	1229:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL)	1229:1395	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	45	theme	3-ethylbenzothiazoline-6-sulfonic	1256:1288	arg1	acid					1290:1293	3-ethylbenzothiazoline-6-sulfonic acid	1256:1293	3-ethylbenzothiazoline-6-sulfonic acid	1256:1293	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	2	46	theme	drying	457:462	arg1	technologies					464:475	different drying technologies	447:475	different drying technologies	447:475	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	2	47	theme	molar	383:387	arg1	ratios					389:394	molar ratios	383:394	molar ratios	383:394	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	7	48	theme	scavenging	1460:1469	arg1	ability					1471:1477	2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability	1401:1477	2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL)	1401:1566	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	48	theme	scavenging	1460:1469	arg1	values					1485:1490	IC50 values	1480:1490	IC50 values	1480:1490	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	49	theme	2,2-azidobisphenol	1236:1253	arg1	ability					1315:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability	1229:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL)	1229:1395	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	49	theme	2,2-azidobisphenol	1236:1253	arg1	values					1329:1334	IC50 values	1324:1334	IC50 values	1324:1334	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	49	theme	2,2-azidobisphenol	1236:1253	arg1	acid					1290:1293	3-ethylbenzothiazoline-6-sulfonic acid	1256:1293	3-ethylbenzothiazoline-6-sulfonic acid	1256:1293	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	3	50	theme	Low	478:480	arg1	weight					492:497	Low molecular weight	478:497	Low molecular weight distributions	478:511	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	0	51	theme	Soluble	46:52	arg1	Polysaccharides					54:68	Water Soluble Polysaccharides	40:68	Water Soluble Polysaccharides	40:68	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.					
34361549	7	52	theme	hydrazine	1442:1450	arg1	ability					1471:1477	2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability	1401:1477	2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL)	1401:1566	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	52	theme	hydrazine	1442:1450	arg1	values					1485:1490	IC50 values	1480:1490	IC50 values	1480:1490	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	53	theme	inhibitory	1705:1714	arg1	effects					1716:1722	inhibitory effects	1705:1722	inhibitory effects	1705:1722	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	1	54	theme	biological	203:212	arg1	properties					214:223	the structural and biological properties	184:223	the structural and biological properties of polysaccharides from lotus leaves (LLPs)	184:267	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	7	55	theme	IC50	1742:1745	arg1	values					1747:1752	IC50 values	1742:1752	IC50 values	1742:1752	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	3	56	theme	radio	630:634	arg1	LLP-RF					654:659	LLP-RF	654:659	LLP-RF	654:659	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	3	56	theme	radio	630:634	arg1	drying					646:651	radio frequency drying	630:651	radio frequency drying (LLP-RF)	630:660	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	2	57	theme	components	348:357	arg1	weights					370:376	molecular weights	360:376	molecular weights	360:376	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	2	57	theme	components	348:357	arg1	contents					321:328	contents	321:328	contents of basic chemical components	321:357	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	2	57	theme	components	348:357	arg1	ratios					389:394	molar ratios	383:394	molar ratios	383:394	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	2	57	theme	components	348:357	arg1	yields					313:318	the yields	309:318	the yields	309:318	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	8	58	theme	industrial	1974:1983	arg1	applications					1985:1996	industrial applications	1974:1996	industrial applications	1974:1996	These findings indicate that both hot air drying and microwave drying can be potential drying techniques for the pre-processing of lotus leaves for industrial applications.					
34361549	2	59	theme	basic	333:337	arg1	components					348:357	basic chemical components	333:357	basic chemical components	333:357	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	4	60	theme	vacuum	809:814	arg1	drying					816:821	vacuum drying	809:821	vacuum drying (LLP-V)	809:829	The high contents of bound polyphenolics were measured in LLP-H and LLP-M, as well as polysaccharides obtained from lotus leaves by vacuum drying (LLP-V).					
34361549	4	60	theme	vacuum	809:814	arg1	LLP-V					824:828	LLP-V	824:828	LLP-V	824:828	The high contents of bound polyphenolics were measured in LLP-H and LLP-M, as well as polysaccharides obtained from lotus leaves by vacuum drying (LLP-V).					
34361549	7	61	theme	0.043	1376:1380	arg1	mg/mL					1390:1394	0.043 ± 0.002 mg/mL	1376:1394	0.043 ± 0.002 mg/mL	1376:1394	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	8	62	theme	microwave	1879:1887	arg1	drying					1889:1894	microwave drying	1879:1894	microwave drying	1879:1894	These findings indicate that both hot air drying and microwave drying can be potential drying techniques for the pre-processing of lotus leaves for industrial applications.					
34361549	7	63	theme	±	1382:1382	arg1	mg/mL					1390:1394	0.043 ± 0.002 mg/mL	1376:1394	0.043 ± 0.002 mg/mL	1376:1394	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	1	64	theme	drying	163:168	arg1	techniques					170:179	five drying techniques	158:179	five drying techniques	158:179	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	6	65	theme	biological	1072:1081	arg1	effects					1179:1185	inhibitory effects	1168:1185	inhibitory effects on α-glucosidase	1168:1202	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	6	65	theme	biological	1072:1081	arg1	properties					1083:1092	obvious biological properties	1064:1092	obvious biological properties	1064:1092	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	6	65	theme	biological	1072:1081	arg1	capacities					1126:1135	in vitro antioxidant capacities	1105:1135	in vitro antioxidant capacities	1105:1135	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	6	65	theme	biological	1072:1081	arg1	activities					1152:1161	antiglycation activities	1138:1161	antiglycation activities	1138:1161	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	1	66	theme	structural	188:197	arg1	properties					214:223	the structural and biological properties	184:223	the structural and biological properties of polysaccharides from lotus leaves (LLPs)	184:267	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	0	67	from	Lotus	75:79	arg1	Properties					26:35	Structural and Biological Properties	0:35	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.	0:117	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.					
34361549	7	68	theme	0.176	1344:1348	arg1	mg/mL					1358:1362	0.176 ± 0.004 mg/mL	1344:1362	0.176 ± 0.004 mg/mL	1344:1362	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	68	theme	0.176	1344:1348	arg1	LLP-H					1337:1341	LLP-H	1337:1341	LLP-H	1337:1341	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	6	69	dep	in	1105:1106	arg1	vitro					1108:1112	vitro	1108:1112	vitro	1108:1112	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	3	70	theme	air	580:582	arg1	LLP-H					592:596	LLP-H	592:596	LLP-H	592:596	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	3	70	theme	air	580:582	arg1	drying					584:589	hot air drying	576:589	hot air drying (LLP-H)	576:597	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	7	71	dep	exerted	1591:1597	arg1	values					1747:1752	IC50 values	1742:1752	IC50 values	1742:1752	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	3	72	theme	microwave	600:608	arg1	LLP-M					618:622	LLP-M	618:622	LLP-M	618:622	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	3	72	theme	microwave	600:608	arg1	drying					610:615	microwave drying	600:615	microwave drying (LLP-M)	600:623	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	7	73	theme	1.023	1643:1647	arg1	±					1649:1649	±	1649:1649	±	1649:1649	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	8	74	dep	lotus	1957:1961	arg1	leaves					1963:1968	leaves	1963:1968	leaves	1963:1968	These findings indicate that both hot air drying and microwave drying can be potential drying techniques for the pre-processing of lotus leaves for industrial applications.					
34361549	3	75	dep	lotus	560:564	arg1	leaves					566:571	leaves	566:571	leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively	566:674	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	6	76	theme	antioxidant	1114:1124	arg1	capacities					1126:1135	in vitro antioxidant capacities	1105:1135	in vitro antioxidant capacities	1105:1135	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	6	77	from	capacities	1126:1135	arg1	α-glucosidase					1190:1202	α-glucosidase	1190:1202	α-glucosidase	1190:1202	Besides, all LLPs exhibited obvious biological properties, including in vitro antioxidant capacities, antiglycation activities, and inhibitory effects on α-glucosidase.					
34361549	7	78	theme	±	1798:1798	arg1	μg/mL					1806:1810	724.98 ± 16.93 μg/mL	1791:1810	724.98 ± 16.93 μg/mL	1791:1810	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	1	79	from	influence	145:153	arg1	properties					214:223	the structural and biological properties	184:223	the structural and biological properties of polysaccharides from lotus leaves (LLPs)	184:267	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
34361549	5	80	theme	nuclear	889:895	arg1	NMR					917:919	NMR	917:919	NMR	917:919	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	5	80	theme	nuclear	889:895	arg1	resonance					906:914	nuclear magnetic resonance	889:914	nuclear magnetic resonance (NMR) spectra of LLPs	889:936	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	0	81	theme	Techniques	107:116	arg1	Effects					89:95	Effects	89:95	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.	0:117	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.					
34361549	5	82	theme	chemical	1015:1022	arg1	structures					1024:1033	their basic chemical structures	1003:1033	their basic chemical structures	1003:1033	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	5	83	theme	resonance	906:914	arg1	spectra					922:928	nuclear magnetic resonance (NMR) spectra	889:928	nuclear magnetic resonance (NMR) spectra of LLPs	889:936	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	7	84	theme	stronger	1599:1606	arg1	antiglycation					1608:1620	stronger antiglycation	1599:1620	stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL)	1599:1699	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	84	theme	stronger	1599:1606	arg1	values					1628:1633	IC50 values	1623:1633	IC50 values	1623:1633	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	85	theme	radical	1296:1302	arg1	ability					1315:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability	1229:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL)	1229:1395	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	85	theme	radical	1296:1302	arg1	values					1329:1334	IC50 values	1324:1334	IC50 values	1324:1334	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	85	theme	radical	1296:1302	arg1	acid					1290:1293	3-ethylbenzothiazoline-6-sulfonic acid	1256:1293	3-ethylbenzothiazoline-6-sulfonic acid	1256:1293	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	5	86	dep	Fourier	850:856	arg1	transform					858:866	transform	858:866	transform infrared	858:875	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	7	87	theme	0.02	1769:1772	arg1	±					1767:1767	±	1767:1767	±	1767:1767	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	8	88	theme	drying	1913:1918	arg1	techniques					1920:1929	potential drying techniques	1903:1929	potential drying techniques for the pre-processing of lotus leaves for industrial applications	1903:1996	These findings indicate that both hot air drying and microwave drying can be potential drying techniques for the pre-processing of lotus leaves for industrial applications.					
34361549	4	89	dep	lotus	793:797	arg1	leaves					799:804	leaves	799:804	leaves by vacuum drying (LLP-V)	799:829	The high contents of bound polyphenolics were measured in LLP-H and LLP-M, as well as polysaccharides obtained from lotus leaves by vacuum drying (LLP-V).					
34361549	7	90	dep	2,2-diphenyl-1-	1401:1415	arg1	phenyl					1434:1439	2,4,6-trinitrate phenyl	1417:1439	2,4,6-trinitrate phenyl	1417:1439	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	91	theme	1.90	1762:1765	arg1	±					1767:1767	±	1767:1767	±	1767:1767	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	0	92	dep	Lotus	75:79	arg1	Leaves					81:86	Leaves	81:86	Leaves	81:86	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.					
34361549	2	93	theme	compositional	399:411	arg1	monosaccharides					413:427	compositional monosaccharides	399:427	compositional monosaccharides of LLPs	399:435	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	4	94	theme	bound	698:702	arg1	polyphenolics					704:716	bound polyphenolics	698:716	bound polyphenolics	698:716	The high contents of bound polyphenolics were measured in LLP-H and LLP-M, as well as polysaccharides obtained from lotus leaves by vacuum drying (LLP-V).					
34361549	0	95	theme	Water	40:44	arg1	Polysaccharides					54:68	Water Soluble Polysaccharides	40:68	Water Soluble Polysaccharides	40:68	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.					
34361549	7	96	theme	IC50	1480:1483	arg1	ability					1471:1477	2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability	1401:1477	2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL)	1401:1566	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	96	theme	IC50	1480:1483	arg1	values					1485:1490	IC50 values	1480:1490	IC50 values	1480:1490	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	5	97	theme	LLPs	933:936	arg1	spectra					922:928	nuclear magnetic resonance (NMR) spectra	889:928	nuclear magnetic resonance (NMR) spectra of LLPs	889:936	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	5	97	theme	LLPs	933:936	arg1	Fourier					850:856	Fourier	850:856	Fourier transform infrared (FT-IR)	850:883	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	5	97	theme	LLPs	933:936	arg1	FT-IR					878:882	FT-IR	878:882	FT-IR	878:882	Furthermore, both Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra of LLPs were similar, indicating that drying technologies did not change their basic chemical structures.					
34361549	7	98	theme	higher	1229:1234	arg1	ability					1315:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability	1229:1321	higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL)	1229:1395	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	98	theme	higher	1229:1234	arg1	values					1329:1334	IC50 values	1324:1334	IC50 values	1324:1334	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	98	theme	higher	1229:1234	arg1	acid					1290:1293	3-ethylbenzothiazoline-6-sulfonic acid	1256:1293	3-ethylbenzothiazoline-6-sulfonic acid	1256:1293	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	2	99	theme	different	447:455	arg1	technologies					464:475	different drying technologies	447:475	different drying technologies	447:475	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	0	100	theme	Polysaccharides	54:68	arg1	Properties					26:35	Structural and Biological Properties	0:35	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.	0:117	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.					
34361549	8	101	theme	lotus	1957:1961	arg1	pre-processing					1939:1952	pre-processing	1939:1952	pre-processing	1939:1952	These findings indicate that both hot air drying and microwave drying can be potential drying techniques for the pre-processing of lotus leaves for industrial applications.					
34361549	3	102	theme	frequency	636:644	arg1	LLP-RF					654:659	LLP-RF	654:659	LLP-RF	654:659	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	3	102	theme	frequency	636:644	arg1	drying					646:651	radio frequency drying	630:651	radio frequency drying (LLP-RF)	630:660	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	3	103	theme	molecular	482:490	arg1	weight					492:497	Low molecular weight	478:497	Low molecular weight distributions	478:511	Low molecular weight distributions were observed in polysaccharides obtained from lotus leaves by hot air drying (LLP-H), microwave drying (LLP-M), and radio frequency drying (LLP-RF), respectively.					
34361549	7	104	theme	radical	1452:1458	arg1	ability					1471:1477	2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability	1401:1477	2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL)	1401:1566	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	7	104	theme	radical	1452:1458	arg1	values					1485:1490	IC50 values	1480:1490	IC50 values	1480:1490	Indeed, LLP-H exhibited higher 2,2-azidobisphenol (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability (IC50 values, LLP-H, 0.176 ± 0.004 mg/mL; vitamin C, 0.043 ± 0.002 mg/mL) and 2,2-diphenyl-1-(2,4,6-trinitrate phenyl) hydrazine radical scavenging ability (IC50 values, LLP-H, 0.241 ± 0.007 mg/mL; butylated hydroxytoluene, 0.366 ± 0.010 mg/mL) than others, and LLP-M exerted stronger antiglycation (IC50 values, LLP-M, 1.023 ± 0.053 mg/mL; aminoguanidine, 1.744 ± 0.080 mg/mL) and inhibitory effects on α-glucosidase (IC50 values, LLP-M, 1.90 ± 0.02 μg/mL; acarbose, 724.98 ± 16.93 μg/mL) than others.					
34361549	0	105	dep	Properties	26:35	arg1	Effects					89:95	Effects	89:95	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.	0:117	Structural and Biological Properties of Water Soluble Polysaccharides from Lotus Leaves: Effects of Drying Techniques.					
34361549	2	106	theme	chemical	339:346	arg1	components					348:357	basic chemical components	333:357	basic chemical components	333:357	Results revealed that the yields, contents of basic chemical components, molecular weights, and molar ratios of compositional monosaccharides of LLPs varied by different drying technologies.					
34361549	1	107	theme	polysaccharides	228:242	arg1	properties					214:223	the structural and biological properties	184:223	the structural and biological properties of polysaccharides from lotus leaves (LLPs)	184:267	In the present study, the influence of five drying techniques on the structural and biological properties of polysaccharides from lotus leaves (LLPs) was investigated.					
31923518	11	0	theme	weight	1434:1439	arg1	ratio					1441:1445	weight ratio	1434:1445	weight ratio	1434:1445	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	11	0	theme	weight	1434:1439	arg1	PT/CS					1428:1432	3.75 PT/CS	1423:1432	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content	1423:1478	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	8	1	from	14 days	1085:1091	arg1	buffer					1106:1111	phosphate buffer	1096:1111	phosphate buffer	1096:1111	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	9	2	dep	ratio	1166:1170	arg1	disintegrate					1172:1183	disintegrate	1172:1183	3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4)	1138:1271	Composites obtained at 3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4).					
31923518	11	3	theme	hydrogel	1534:1541	arg1	composite					1543:1551	a durable, cytocompatible, and superabsorbent hydrogel composite	1488:1551	a durable, cytocompatible, and superabsorbent hydrogel composite	1488:1551	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	1	4	theme	gold	177:180	arg1	AuNPs					197:201	AuNPs	197:201	AuNPs	197:201	Studies report the production of gold nanoparticles (AuNPs) and polysaccharides-based composites.					
31923518	1	4	theme	gold	177:180	arg1	nanoparticles					182:194	gold nanoparticles	177:194	gold nanoparticles (AuNPs)	177:202	Studies report the production of gold nanoparticles (AuNPs) and polysaccharides-based composites.					
31923518	10	5	theme	hydrogel	1298:1305	arg1	structures					1307:1316	the hydrogel structures	1294:1316	the hydrogel structures	1294:1316	The AuNPs reinforce the hydrogel structures, increasing the elastic modulus (from 3.5 to 7.6 Pa) and decreasing the water uptake from 4465 to 2976%.					
31923518	4	6	dep	spectroscopy	499:510	arg1	measurements					541:552	measurements	541:552	measurements	541:552	Visible spectroscopy and dynamic light scattering measurements confirm the AuNPs synthesis.					
31923518	0	7	theme	hydrogel	109:116	arg1	composites					118:127	durable, cytocompatible, and superabsorbent hydrogel composites	65:127	durable, cytocompatible, and superabsorbent hydrogel composites	65:127	Pectin-capped gold nanoparticles synthesis in-situ for producing durable, cytocompatible, and superabsorbent hydrogel composites with chitosan.					
31923518	4	8	theme	AuNPs	566:570	arg1	synthesis					572:580	the AuNPs synthesis	562:580	the AuNPs synthesis	562:580	Visible spectroscopy and dynamic light scattering measurements confirm the AuNPs synthesis.					
31923518	10	9	theme	elastic	1334:1340	arg1	modulus					1342:1348	the elastic modulus	1330:1348	the elastic modulus (from 3.5 to 7.6 Pa)	1330:1369	The AuNPs reinforce the hydrogel structures, increasing the elastic modulus (from 3.5 to 7.6 Pa) and decreasing the water uptake from 4465 to 2976%.					
31923518	9	10	theme	pectin/chitosan	1143:1157	arg1	ratio					1166:1170	3.75 pectin/chitosan weight ratio	1138:1170	3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4)	1138:1271	Composites obtained at 3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4).					
31923518	12	11	theme	delivery	1602:1609	arg1	purposes					1611:1618	drug delivery purposes	1597:1618	drug delivery purposes	1597:1618	These properties can support materials for drug delivery purposes.					
31923518	0	12	theme	superabsorbent	94:107	arg1	composites					118:127	durable, cytocompatible, and superabsorbent hydrogel composites	65:127	durable, cytocompatible, and superabsorbent hydrogel composites	65:127	Pectin-capped gold nanoparticles synthesis in-situ for producing durable, cytocompatible, and superabsorbent hydrogel composites with chitosan.					
31923518	4	13	theme	light	524:528	arg1	scattering					530:539	dynamic light scattering	516:539	dynamic light scattering	516:539	Visible spectroscopy and dynamic light scattering measurements confirm the AuNPs synthesis.					
31923518	6	14	theme	compact	781:787	arg1	structures					789:798	compact structures	781:798	compact structures	781:798	Scanning electron microscopy shows that the composites present compact structures.					
31923518	12	15	theme	drug	1597:1600	arg1	purposes					1611:1618	drug delivery purposes	1597:1618	drug delivery purposes	1597:1618	These properties can support materials for drug delivery purposes.					
31923518	11	16	theme	Au	1464:1465	arg1	content					1472:1478	3.0 × 10-4 M Au(III) content	1451:1478	3.0 × 10-4 M Au(III) content	1451:1478	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	11	16	theme	Au	1464:1465	arg1	PT/CS					1428:1432	3.75 PT/CS	1423:1432	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content	1423:1478	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	4	17	theme	dynamic	516:522	arg1	scattering					530:539	dynamic light scattering	516:539	dynamic light scattering	516:539	Visible spectroscopy and dynamic light scattering measurements confirm the AuNPs synthesis.					
31923518	9	18	theme	phosphate	1221:1229	arg1	buffer					1231:1236	phosphate buffer	1221:1236	phosphate buffer (physiological condition = pH 7.4)	1221:1271	Composites obtained at 3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4).					
31923518	9	18	theme	phosphate	1221:1229	arg1	condition = pH 7.4					1253:1270	physiological condition = pH 7.4	1239:1270	physiological condition = pH 7.4	1239:1270	Composites obtained at 3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4).					
31923518	11	19	theme	durable	1490:1496	arg1	composite					1543:1551	a durable, cytocompatible, and superabsorbent hydrogel composite	1488:1551	a durable, cytocompatible, and superabsorbent hydrogel composite	1488:1551	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	1	20	theme	nanoparticles	182:194	arg1	production					163:172	the production	159:172	the production of gold nanoparticles (AuNPs) and polysaccharides-based composites	159:239	Studies report the production of gold nanoparticles (AuNPs) and polysaccharides-based composites.					
31923518	8	21	contain	containing	950:959	arg2	ratio					1020:1024	at 4.12 pectin/chitosan weight ratio	989:1024	at 4.12 pectin/chitosan weight ratio	989:1024	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	8	21	contain	containing	950:959	arg1	Hydrogels					916:924	Hydrogels	916:924	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio)	916:1025	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	8	21	contain	containing	950:959	arg2	content					980:986	the highest pectin content	961:986	the highest pectin content (at 4.12 pectin/chitosan weight ratio)	961:1025	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	5	22	theme	Zeta	685:688	arg1	potential					690:698	the Zeta potential	681:698	the Zeta potential	681:698	The hydrodynamic radius of the pectin-capped AuNPs ranges from approximately 510 to 721 nm, while the Zeta potential is around -43 mV.					
31923518	0	23	theme	Pectin-capped	0:12	arg1	gold					14:17	Pectin-capped gold	0:17	Pectin-capped gold	0:17	Pectin-capped gold nanoparticles synthesis in-situ for producing durable, cytocompatible, and superabsorbent hydrogel composites with chitosan.					
31923518	8	24	theme	pectin	973:978	arg1	ratio					1020:1024	at 4.12 pectin/chitosan weight ratio	989:1024	at 4.12 pectin/chitosan weight ratio	989:1024	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	8	24	theme	pectin	973:978	arg1	content					980:986	the highest pectin content	961:986	the highest pectin content (at 4.12 pectin/chitosan weight ratio)	961:1025	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	2	25	theme	hydrogel	332:339	arg1	composites					341:350	hydrogel composites	332:350	hydrogel composites	332:350	However, there are few reports about AuNPs synthesis in-situ followed by the formation of hydrogel composites.					
31923518	8	26	theme	highest	965:971	arg1	ratio					1020:1024	at 4.12 pectin/chitosan weight ratio	989:1024	at 4.12 pectin/chitosan weight ratio	989:1024	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	8	26	theme	highest	965:971	arg1	content					980:986	the highest pectin content	961:986	the highest pectin content (at 4.12 pectin/chitosan weight ratio)	961:1025	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	3	27	theme	pectin-capped	441:453	arg1	composites					479:488	cytocompatible pectin-capped AuNPs/chitosan hydrogel composites	426:488	cytocompatible pectin-capped AuNPs/chitosan hydrogel composites	426:488	Here, we show AuNPs synthesis in-situ into the pectin solutions to yield cytocompatible pectin-capped AuNPs/chitosan hydrogel composites.					
31923518	3	28	theme	pectin	400:405	arg1	solutions					407:415	the pectin solutions	396:415	the pectin solutions	396:415	Here, we show AuNPs synthesis in-situ into the pectin solutions to yield cytocompatible pectin-capped AuNPs/chitosan hydrogel composites.					
31923518	10	29	from	%	1420:1420	arg1	uptake					1396:1401	the water uptake	1386:1401	the water uptake from 4465 to 2976%	1386:1420	The AuNPs reinforce the hydrogel structures, increasing the elastic modulus (from 3.5 to 7.6 Pa) and decreasing the water uptake from 4465 to 2976%.					
31923518	11	30	dep	PT/CS	1428:1432	arg1	content					1472:1478	3.0 × 10-4 M Au(III) content	1451:1478	3.0 × 10-4 M Au(III) content	1451:1478	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	11	30	dep	PT/CS	1428:1432	arg1	ratio					1441:1445	weight ratio	1434:1445	weight ratio	1434:1445	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	11	30	dep	PT/CS	1428:1432	arg1	PT/CS					1428:1432	3.75 PT/CS	1423:1432	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content	1423:1478	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	8	31	theme	phosphate	1096:1104	arg1	buffer					1106:1111	phosphate buffer	1096:1111	phosphate buffer	1096:1111	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	3	32	theme	AuNPs/chitosan	455:468	arg1	composites					479:488	cytocompatible pectin-capped AuNPs/chitosan hydrogel composites	426:488	cytocompatible pectin-capped AuNPs/chitosan hydrogel composites	426:488	Here, we show AuNPs synthesis in-situ into the pectin solutions to yield cytocompatible pectin-capped AuNPs/chitosan hydrogel composites.					
31923518	10	33	from	3.5	1356:1358	arg1	modulus					1342:1348	the elastic modulus	1330:1348	the elastic modulus (from 3.5 to 7.6 Pa)	1330:1369	The AuNPs reinforce the hydrogel structures, increasing the elastic modulus (from 3.5 to 7.6 Pa) and decreasing the water uptake from 4465 to 2976%.					
31923518	10	34	theme	water	1390:1394	arg1	uptake					1396:1401	the water uptake	1386:1401	the water uptake from 4465 to 2976%	1386:1420	The AuNPs reinforce the hydrogel structures, increasing the elastic modulus (from 3.5 to 7.6 Pa) and decreasing the water uptake from 4465 to 2976%.					
31923518	8	35	contain	have	1027:1030	arg2	disintegrates					1047:1059	disintegrates approximately 60% after 14 days in phosphate buffer	1047:1111	disintegrates approximately 60% after 14 days in phosphate buffer	1047:1111	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	8	35	contain	have	1027:1030	arg2	stability					1036:1044	low stability	1032:1044	low stability (disintegrates approximately 60% after 14 days in phosphate buffer)	1032:1112	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	8	35	contain	have	1027:1030	arg1	Hydrogels					916:924	Hydrogels	916:924	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio)	916:1025	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	4	36	theme	Visible	491:497	arg1	spectroscopy					499:510	Visible spectroscopy	491:510	Visible spectroscopy	491:510	Visible spectroscopy and dynamic light scattering measurements confirm the AuNPs synthesis.					
31923518	8	37	dep	disintegrates	1047:1059	arg1	%					1077:1077	approximately 60%	1061:1077	disintegrates approximately 60% after 14 days in phosphate buffer	1047:1111	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	10	38	dep	2976	1416:1419	arg1	to					1413:1414	to	1413:1414	to	1413:1414	The AuNPs reinforce the hydrogel structures, increasing the elastic modulus (from 3.5 to 7.6 Pa) and decreasing the water uptake from 4465 to 2976%.					
31923518	1	39	theme	polysaccharides-based	208:228	arg1	composites					230:239	polysaccharides-based composites	208:239	polysaccharides-based composites	208:239	Studies report the production of gold nanoparticles (AuNPs) and polysaccharides-based composites.					
31923518	11	40	theme	cytocompatible	1499:1512	arg1	composite					1543:1551	a durable, cytocompatible, and superabsorbent hydrogel composite	1488:1551	a durable, cytocompatible, and superabsorbent hydrogel composite	1488:1551	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	10	41	dep	reinforce	1284:1292	arg1	increasing					1319:1328	increasing	1319:1328	increasing the elastic modulus (from 3.5 to 7.6 Pa)	1319:1369	The AuNPs reinforce the hydrogel structures, increasing the elastic modulus (from 3.5 to 7.6 Pa) and decreasing the water uptake from 4465 to 2976%.					
31923518	10	41	dep	reinforce	1284:1292	arg1	decreasing					1375:1384	decreasing	1375:1384	decreasing the water uptake from 4465 to 2976%	1375:1420	The AuNPs reinforce the hydrogel structures, increasing the elastic modulus (from 3.5 to 7.6 Pa) and decreasing the water uptake from 4465 to 2976%.					
31923518	6	42	theme	electron	727:734	arg1	microscopy					736:745	Scanning electron microscopy	718:745	Scanning electron microscopy	718:745	Scanning electron microscopy shows that the composites present compact structures.					
31923518	1	43	theme	composites	230:239	arg1	production					163:172	the production	159:172	the production of gold nanoparticles (AuNPs) and polysaccharides-based composites	159:239	Studies report the production of gold nanoparticles (AuNPs) and polysaccharides-based composites.					
31923518	3	44	theme	hydrogel	470:477	arg1	composites					479:488	cytocompatible pectin-capped AuNPs/chitosan hydrogel composites	426:488	cytocompatible pectin-capped AuNPs/chitosan hydrogel composites	426:488	Here, we show AuNPs synthesis in-situ into the pectin solutions to yield cytocompatible pectin-capped AuNPs/chitosan hydrogel composites.					
31923518	3	45	theme	cytocompatible	426:439	arg1	composites					479:488	cytocompatible pectin-capped AuNPs/chitosan hydrogel composites	426:488	cytocompatible pectin-capped AuNPs/chitosan hydrogel composites	426:488	Here, we show AuNPs synthesis in-situ into the pectin solutions to yield cytocompatible pectin-capped AuNPs/chitosan hydrogel composites.					
31923518	8	46	theme	weight	1013:1018	arg1	ratio					1020:1024	at 4.12 pectin/chitosan weight ratio	989:1024	at 4.12 pectin/chitosan weight ratio	989:1024	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	8	46	theme	weight	1013:1018	arg1	content					980:986	the highest pectin content	961:986	the highest pectin content (at 4.12 pectin/chitosan weight ratio)	961:1025	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	9	47	from	14 days	1210:1216	arg1	buffer					1231:1236	phosphate buffer	1221:1236	phosphate buffer (physiological condition = pH 7.4)	1221:1271	Composites obtained at 3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4).					
31923518	9	47	from	14 days	1210:1216	arg1	condition = pH 7.4					1253:1270	physiological condition = pH 7.4	1239:1270	physiological condition = pH 7.4	1239:1270	Composites obtained at 3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4).					
31923518	0	48	theme	durable	65:71	arg1	composites					118:127	durable, cytocompatible, and superabsorbent hydrogel composites	65:127	durable, cytocompatible, and superabsorbent hydrogel composites	65:127	Pectin-capped gold nanoparticles synthesis in-situ for producing durable, cytocompatible, and superabsorbent hydrogel composites with chitosan.					
31923518	3	49	theme	AuNPs	367:371	arg1	synthesis					373:381	AuNPs synthesis	367:381	AuNPs synthesis in-situ into the pectin solutions to yield cytocompatible pectin-capped AuNPs/chitosan hydrogel composites	367:488	Here, we show AuNPs synthesis in-situ into the pectin solutions to yield cytocompatible pectin-capped AuNPs/chitosan hydrogel composites.					
31923518	8	50	theme	pectin/chitosan	997:1011	arg1	ratio					1020:1024	at 4.12 pectin/chitosan weight ratio	989:1024	at 4.12 pectin/chitosan weight ratio	989:1024	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	8	50	theme	pectin/chitosan	997:1011	arg1	content					980:986	the highest pectin content	961:986	the highest pectin content (at 4.12 pectin/chitosan weight ratio)	961:1025	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	7	51	theme	X-ray	846:850	arg1	spectroscopy					866:877	X-ray photoelectron spectroscopy	846:877	X-ray photoelectron spectroscopy	846:877	Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy characterize the composites as well.					
31923518	5	52	theme	pectin-capped	614:626	arg1	AuNPs					628:632	the pectin-capped AuNPs	610:632	the pectin-capped AuNPs	610:632	The hydrodynamic radius of the pectin-capped AuNPs ranges from approximately 510 to 721 nm, while the Zeta potential is around -43 mV.					
31923518	6	53	theme	Scanning	718:725	arg1	microscopy					736:745	Scanning electron microscopy	718:745	Scanning electron microscopy	718:745	Scanning electron microscopy shows that the composites present compact structures.					
31923518	7	54	theme	photoelectron	852:864	arg1	spectroscopy					866:877	X-ray photoelectron spectroscopy	846:877	X-ray photoelectron spectroscopy	846:877	Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy characterize the composites as well.					
31923518	0	55	dep	synthesis	33:41	arg1	in-situ					43:49	synthesis in-situ	33:49	synthesis in-situ for producing durable, cytocompatible, and superabsorbent hydrogel composites with chitosan	33:141	Pectin-capped gold nanoparticles synthesis in-situ for producing durable, cytocompatible, and superabsorbent hydrogel composites with chitosan.					
31923518	2	56	theme	AuNPs	279:283	arg1	synthesis					285:293	AuNPs synthesis	279:293	AuNPs synthesis in-situ followed by the formation of hydrogel composites	279:350	However, there are few reports about AuNPs synthesis in-situ followed by the formation of hydrogel composites.					
31923518	5	57	theme	AuNPs	628:632	arg1	radius					600:605	The hydrodynamic radius	583:605	The hydrodynamic radius of the pectin-capped AuNPs	583:632	The hydrodynamic radius of the pectin-capped AuNPs ranges from approximately 510 to 721 nm, while the Zeta potential is around -43 mV.					
31923518	9	58	theme	physiological	1239:1251	arg1	buffer					1231:1236	phosphate buffer	1221:1236	phosphate buffer (physiological condition = pH 7.4)	1221:1271	Composites obtained at 3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4).					
31923518	9	58	theme	physiological	1239:1251	arg1	condition = pH 7.4					1253:1270	physiological condition = pH 7.4	1239:1270	physiological condition = pH 7.4	1239:1270	Composites obtained at 3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4).					
31923518	3	59	dep	synthesis	373:381	arg1	in-situ					383:389	AuNPs synthesis in-situ	367:389	AuNPs synthesis in-situ into the pectin solutions to yield cytocompatible pectin-capped AuNPs/chitosan hydrogel composites	367:488	Here, we show AuNPs synthesis in-situ into the pectin solutions to yield cytocompatible pectin-capped AuNPs/chitosan hydrogel composites.					
31923518	8	60	theme	low	1032:1034	arg1	disintegrates					1047:1059	disintegrates approximately 60% after 14 days in phosphate buffer	1047:1111	disintegrates approximately 60% after 14 days in phosphate buffer	1047:1111	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	8	60	theme	low	1032:1034	arg1	stability					1036:1044	low stability	1032:1044	low stability (disintegrates approximately 60% after 14 days in phosphate buffer)	1032:1112	Hydrogels (with or without AuNPs) containing the highest pectin content (at 4.12 pectin/chitosan weight ratio) have low stability (disintegrates approximately 60% after 14 days in phosphate buffer).					
31923518	2	61	theme	composites	341:350	arg1	formation					319:327	the formation	315:327	the formation of hydrogel composites	315:350	However, there are few reports about AuNPs synthesis in-situ followed by the formation of hydrogel composites.					
31923518	11	62	theme	superabsorbent	1519:1532	arg1	composite					1543:1551	a durable, cytocompatible, and superabsorbent hydrogel composite	1488:1551	a durable, cytocompatible, and superabsorbent hydrogel composite	1488:1551	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	0	63	theme	cytocompatible	74:87	arg1	composites					118:127	durable, cytocompatible, and superabsorbent hydrogel composites	65:127	durable, cytocompatible, and superabsorbent hydrogel composites	65:127	Pectin-capped gold nanoparticles synthesis in-situ for producing durable, cytocompatible, and superabsorbent hydrogel composites with chitosan.					
31923518	7	64	dep	transform	809:817	arg1	characterize					879:890	characterize	879:890	transform infrared spectroscopy, and X-ray photoelectron spectroscopy characterize the composites as well	809:913	Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy characterize the composites as well.					
31923518	5	65	dep	721 nm	667:672	arg1	to					664:665	to	664:665	to	664:665	The hydrodynamic radius of the pectin-capped AuNPs ranges from approximately 510 to 721 nm, while the Zeta potential is around -43 mV.					
31923518	7	66	theme	infrared	819:826	arg1	spectroscopy					828:839	infrared spectroscopy	819:839	infrared spectroscopy	819:839	Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy characterize the composites as well.					
31923518	11	67	theme	3.0 × 10-4 M	1451:1462	arg1	content					1472:1478	3.0 × 10-4 M Au(III) content	1451:1478	3.0 × 10-4 M Au(III) content	1451:1478	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	11	67	theme	3.0 × 10-4 M	1451:1462	arg1	PT/CS					1428:1432	3.75 PT/CS	1423:1432	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content	1423:1478	3.75 PT/CS weight ratio and 3.0 × 10-4 M Au(III) content provide a durable, cytocompatible, and superabsorbent hydrogel composite.					
31923518	2	68	theme	few	261:263	arg1	reports					265:271	few reports	261:271	few reports about AuNPs synthesis in-situ followed by the formation of hydrogel composites	261:350	However, there are few reports about AuNPs synthesis in-situ followed by the formation of hydrogel composites.					
31923518	5	69	theme	hydrodynamic	587:598	arg1	radius					600:605	The hydrodynamic radius	583:605	The hydrodynamic radius of the pectin-capped AuNPs	583:632	The hydrodynamic radius of the pectin-capped AuNPs ranges from approximately 510 to 721 nm, while the Zeta potential is around -43 mV.					
31923518	9	70	theme	weight	1159:1164	arg1	ratio					1166:1170	3.75 pectin/chitosan weight ratio	1138:1170	3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4)	1138:1271	Composites obtained at 3.75 pectin/chitosan weight ratio disintegrate between 25 and 30% after 14 days in phosphate buffer (physiological condition = pH 7.4).					
33885154	2	0	theme	Pickering	363:371	arg1	stabilizers					373:383	Pickering stabilizers	363:383	Pickering stabilizers	363:383	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	2	0	theme	Pickering	363:371	arg1	nanoparticles					324:336	zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles	256:336	zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs)	256:342	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	6	1	theme	phase	1125:1129	arg1	volume					1131:1136	a fixed oil phase volume	1113:1136	a fixed oil phase volume (30%, v/v)	1113:1147	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	6	1	theme	phase	1125:1129	arg1	%					1141:1141	30%	1139:1141	30%	1139:1141	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	8	2	theme	study	1524:1528	arg1	findings					1507:1514	The findings	1503:1514	The findings of this study	1503:1528	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	2	3	theme	soluble	265:271	arg1	composite					314:322	soluble soybean polysaccharide (SSPS) food-grade composite	265:322	soluble soybean polysaccharide (SSPS) food-grade composite	265:322	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	4	4	theme	lower	792:796	arg1	ζ-potential					798:808	much lower ζ-potential	787:808	much lower ζ-potential (about -2 mV)	787:822	At pH 4.0, the dispersions of ZPs exhibited a positive ζ-potential (around at +12 mV); however, zein/SSPS NPs obtained at the same pH had much lower ζ-potential (about -2 mV) further proving that there was electrostatic interaction between SSPS and zein.					
33885154	6	5	theme	fixed	1115:1119	arg1	volume					1131:1136	a fixed oil phase volume	1113:1136	a fixed oil phase volume (30%, v/v)	1113:1147	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	6	5	theme	fixed	1115:1119	arg1	%					1141:1141	30%	1139:1141	30%	1139:1141	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	9	6	theme	zein	1806:1809	arg1	properties					1792:1801	the emulsification properties	1773:1801	the emulsification properties of zein and soluble soybean polysaccharides, and stability	1773:1860	This study showed that the food-grade composite colloidal particles formed by electrostatic interaction can significantly improve the emulsification properties of zein and soluble soybean polysaccharides, and stability.					
33885154	8	7	theme	zein	1598:1601	arg1	field					1636:1640	zein in foods, medicine, or cosmetics field	1598:1640	zein in foods, medicine, or cosmetics field	1598:1640	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	9	8	theme	soluble	1815:1821	arg1	polysaccharides					1831:1845	soluble soybean polysaccharides	1815:1845	soluble soybean polysaccharides	1815:1845	This study showed that the food-grade composite colloidal particles formed by electrostatic interaction can significantly improve the emulsification properties of zein and soluble soybean polysaccharides, and stability.					
33885154	10	9	theme	Pickering	1867:1875	arg1	emulsions					1877:1885	The Pickering emulsions	1863:1885	The Pickering emulsions	1863:1885	The Pickering emulsions have been observed in long-term testing.					
33885154	1	10	theme	bioactive	214:222	arg1	compounds					224:232	delivery bioactive compounds	205:232	delivery bioactive compounds	205:232	Pickering emulsions have received wide attention due to their "surfactant-free" character and the ability of delivery bioactive compounds.					
33885154	2	11	theme	current	242:248	arg1	work					250:253	the current work	238:253	the current work	238:253	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	9	12	theme	polysaccharides	1831:1845	arg1	properties					1792:1801	the emulsification properties	1773:1801	the emulsification properties of zein and soluble soybean polysaccharides, and stability	1773:1860	This study showed that the food-grade composite colloidal particles formed by electrostatic interaction can significantly improve the emulsification properties of zein and soluble soybean polysaccharides, and stability.					
33885154	2	13	theme	composite	314:322	arg1	nanoparticles					324:336	zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles	256:336	zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs)	256:342	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	2	13	theme	composite	314:322	arg1	stabilizers					373:383	Pickering stabilizers	363:383	Pickering stabilizers	363:383	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	2	13	theme	composite	314:322	arg1	NPs					339:341	NPs	339:341	NPs	339:341	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	9	14	theme	food-grade	1670:1679	arg1	particles					1701:1709	the food-grade composite colloidal particles	1666:1709	the food-grade composite colloidal particles formed by electrostatic interaction	1666:1745	This study showed that the food-grade composite colloidal particles formed by electrostatic interaction can significantly improve the emulsification properties of zein and soluble soybean polysaccharides, and stability.					
33885154	5	15	theme	polydispersity	981:994	arg1	PDI					1003:1005	PDI	1003:1005	PDI	1003:1005	The composite nanoparticles (NPs) were well dispersed through the results of polydispersity index (PDI).					
33885154	5	15	theme	polydispersity	981:994	arg1	index					996:1000	polydispersity index	981:1000	polydispersity index (PDI)	981:1006	The composite nanoparticles (NPs) were well dispersed through the results of polydispersity index (PDI).					
33885154	5	16	theme	index	996:1000	arg1	results					970:976	the results	966:976	the results of polydispersity index (PDI)	966:1006	The composite nanoparticles (NPs) were well dispersed through the results of polydispersity index (PDI).					
33885154	8	17	theme	field	1636:1640	arg1	application					1583:1593	the application	1579:1593	the application of zein in foods, medicine, or cosmetics field	1579:1640	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	8	18	theme	zein	1353:1356	arg1	concentration					1358:1370	zein concentration	1353:1370	zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL	1353:1415	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	7	19	theme	scanning	1250:1257	arg1	CLSM					1271:1274	CLSM	1271:1274	CLSM	1271:1274	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	7	19	theme	scanning	1250:1257	arg1	microscopy					1259:1268	confocal laser scanning microscopy	1235:1268	confocal laser scanning microscopy (CLSM)	1235:1275	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	6	20	dep	%	1141:1141	arg1	v/v					1144:1146	v/v	1144:1146	v/v	1144:1146	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	2	21	theme	soybean	273:279	arg1	composite					314:322	soluble soybean polysaccharide (SSPS) food-grade composite	265:322	soluble soybean polysaccharide (SSPS) food-grade composite	265:322	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	1	22	theme	surfactant-free	159:173	arg1	character					176:184	their "surfactant-free" character	152:184	their "surfactant-free" character	152:184	Pickering emulsions have received wide attention due to their "surfactant-free" character and the ability of delivery bioactive compounds.					
33885154	7	23	theme	confocal	1235:1242	arg1	CLSM					1271:1274	CLSM	1271:1274	CLSM	1271:1274	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	7	23	theme	confocal	1235:1242	arg1	microscopy					1259:1268	confocal laser scanning microscopy	1235:1268	confocal laser scanning microscopy (CLSM)	1235:1275	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	10	24	theme	long-term	1909:1917	arg1	testing					1919:1925	long-term testing	1909:1925	long-term testing	1909:1925	The Pickering emulsions have been observed in long-term testing.					
33885154	3	25	theme	electron	615:622	arg1	SEM					636:638	SEM	636:638	SEM	636:638	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	3	25	theme	electron	615:622	arg1	microscopy					624:633	scanning electron microscopy	606:633	the scanning electron microscopy (SEM) images	602:646	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	4	26	theme	ZPs	679:681	arg1	dispersions					664:674	the dispersions	660:674	the dispersions of ZPs	660:681	At pH 4.0, the dispersions of ZPs exhibited a positive ζ-potential (around at +12 mV); however, zein/SSPS NPs obtained at the same pH had much lower ζ-potential (about -2 mV) further proving that there was electrostatic interaction between SSPS and zein.					
33885154	8	27	theme	concentration	1392:1404	arg1	concentration					1358:1370	zein concentration	1353:1370	zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL	1353:1415	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	1	28	theme	Pickering	96:104	arg1	emulsions					106:114	Pickering emulsions	96:114	Pickering emulsions	96:114	Pickering emulsions have received wide attention due to their "surfactant-free" character and the ability of delivery bioactive compounds.					
33885154	8	29	theme	1 mg/mL	1409:1415	arg1	concentration					1392:1404	SSPS concentration	1387:1404	SSPS concentration	1387:1404	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	8	29	theme	1 mg/mL	1409:1415	arg1	6 mg/mL					1375:1381	6 mg/mL	1375:1381	6 mg/mL	1375:1381	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	4	30	dep	ζ-potential	798:808	arg1	-2 mV					817:821	-2 mV	817:821	-2 mV	817:821	At pH 4.0, the dispersions of ZPs exhibited a positive ζ-potential (around at +12 mV); however, zein/SSPS NPs obtained at the same pH had much lower ζ-potential (about -2 mV) further proving that there was electrostatic interaction between SSPS and zein.					
33885154	1	31	theme	wide	130:133	arg1	attention					135:143	wide attention	130:143	wide attention due to their "surfactant-free" character	130:184	Pickering emulsions have received wide attention due to their "surfactant-free" character and the ability of delivery bioactive compounds.					
33885154	3	32	theme	particle	390:397	arg1	size					399:402	The particle size	386:402	The particle size of the composite NPs	386:423	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	8	33	theme	Pickering	1429:1437	arg1	emulsions					1439:1447	the formed Pickering emulsions	1418:1447	the formed Pickering emulsions	1418:1447	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	8	34	from	zein	1598:1601	arg1	cosmetics					1626:1634	cosmetics	1626:1634	cosmetics	1626:1634	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	8	34	from	zein	1598:1601	arg1	foods					1606:1610	foods	1606:1610	foods	1606:1610	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	8	34	from	zein	1598:1601	arg1	medicine					1613:1620	medicine	1613:1620	medicine	1613:1620	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	0	35	theme	zein/soluble	15:26	arg1	polysaccharide					36:49	zein/soluble soybean polysaccharide	15:49	zein/soluble soybean polysaccharide	15:49	Development of zein/soluble soybean polysaccharide nanoparticle-stabilized Pickering emulsions.					
33885154	7	36	theme	interface	1203:1211	arg1	layer					1213:1217	a densely packed interface layer	1186:1217	a densely packed interface layer	1186:1217	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	9	37	theme	colloidal	1691:1699	arg1	particles					1701:1709	the food-grade composite colloidal particles	1666:1709	the food-grade composite colloidal particles formed by electrostatic interaction	1666:1745	This study showed that the food-grade composite colloidal particles formed by electrostatic interaction can significantly improve the emulsification properties of zein and soluble soybean polysaccharides, and stability.					
33885154	4	38	theme	same	775:778	arg1	pH					780:781	the same pH	771:781	the same pH	771:781	At pH 4.0, the dispersions of ZPs exhibited a positive ζ-potential (around at +12 mV); however, zein/SSPS NPs obtained at the same pH had much lower ζ-potential (about -2 mV) further proving that there was electrostatic interaction between SSPS and zein.					
33885154	0	39	theme	polysaccharide	36:49	arg1	Development					0:10	Development	0:10	Development of zein/soluble soybean polysaccharide	0:49	Development of zein/soluble soybean polysaccharide nanoparticle-stabilized Pickering emulsions.					
33885154	3	40	theme	composite	411:419	arg1	NPs					421:423	the composite NPs	407:423	the composite NPs	407:423	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	3	41	theme	larger	497:502	arg1	diameters					517:525	larger hydrodynamic diameters	497:525	larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images	497:646	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	0	42	theme	Pickering	75:83	arg1	emulsions					85:93	Pickering emulsions	75:93	Pickering emulsions	75:93	Development of zein/soluble soybean polysaccharide nanoparticle-stabilized Pickering emulsions.					
33885154	9	43	theme	electrostatic	1721:1733	arg1	interaction					1735:1745	electrostatic interaction	1721:1745	electrostatic interaction	1721:1745	This study showed that the food-grade composite colloidal particles formed by electrostatic interaction can significantly improve the emulsification properties of zein and soluble soybean polysaccharides, and stability.					
33885154	8	44	theme	25	1473:1474	arg1	°C.					1476:1478	°C.	1476:1478	°C.	1476:1478	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	8	45	from	medicine	1613:1620	arg1	zein					1598:1601	zein	1598:1601	zein	1598:1601	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	7	46	theme	oil	1172:1174	arg1	droplets					1176:1183	the oil droplets	1168:1183	the oil droplets	1168:1183	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	8	47	theme	PRACTICAL	1480:1488	arg1	APPLICATION					1490:1500	25 °C. PRACTICAL APPLICATION	1473:1500	25 °C. PRACTICAL APPLICATION	1473:1500	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	8	48	from	foods	1606:1610	arg1	zein					1598:1601	zein	1598:1601	zein	1598:1601	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	9	49	theme	emulsification	1777:1790	arg1	properties					1792:1801	the emulsification properties	1773:1801	the emulsification properties of zein and soluble soybean polysaccharides, and stability	1773:1860	This study showed that the food-grade composite colloidal particles formed by electrostatic interaction can significantly improve the emulsification properties of zein and soluble soybean polysaccharides, and stability.					
33885154	2	50	theme	food-grade	303:312	arg1	composite					314:322	soluble soybean polysaccharide (SSPS) food-grade composite	265:322	soluble soybean polysaccharide (SSPS) food-grade composite	265:322	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	6	51	theme	oil	1121:1123	arg1	volume					1131:1136	a fixed oil phase volume	1113:1136	a fixed oil phase volume (30%, v/v)	1113:1147	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	6	51	theme	oil	1121:1123	arg1	%					1141:1141	30%	1139:1141	30%	1139:1141	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	7	52	theme	droplets	1176:1183	arg1	surface					1157:1163	the surface	1153:1163	the surface of the oil droplets	1153:1183	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	2	53	theme	SSPS	297:300	arg1	composite					314:322	soluble soybean polysaccharide (SSPS) food-grade composite	265:322	soluble soybean polysaccharide (SSPS) food-grade composite	265:322	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	7	54	theme	Ostwald	1332:1338	arg1	ripening					1340:1347	Ostwald ripening	1332:1347	Ostwald ripening	1332:1347	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	8	55	from	cosmetics	1626:1634	arg1	zein					1598:1601	zein	1598:1601	zein	1598:1601	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	1	56	theme	delivery	205:212	arg1	compounds					224:232	delivery bioactive compounds	205:232	delivery bioactive compounds	205:232	Pickering emulsions have received wide attention due to their "surfactant-free" character and the ability of delivery bioactive compounds.					
33885154	7	57	theme	oil	1298:1300	arg1	droplets					1302:1309	oil droplets	1298:1309	oil droplets	1298:1309	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	9	58	theme	soybean	1823:1829	arg1	polysaccharides					1831:1845	soluble soybean polysaccharides	1815:1845	soluble soybean polysaccharides	1815:1845	This study showed that the food-grade composite colloidal particles formed by electrostatic interaction can significantly improve the emulsification properties of zein and soluble soybean polysaccharides, and stability.					
33885154	1	59	theme	compounds	224:232	arg1	ability					194:200	the ability	190:200	the ability of delivery bioactive compounds	190:232	Pickering emulsions have received wide attention due to their "surfactant-free" character and the ability of delivery bioactive compounds.					
33885154	1	59	theme	compounds	224:232	arg1	attention					135:143	wide attention	130:143	wide attention due to their "surfactant-free" character	130:184	Pickering emulsions have received wide attention due to their "surfactant-free" character and the ability of delivery bioactive compounds.					
33885154	4	60	theme	electrostatic	855:867	arg1	interaction					869:879	electrostatic interaction	855:879	electrostatic interaction between SSPS and zein	855:901	At pH 4.0, the dispersions of ZPs exhibited a positive ζ-potential (around at +12 mV); however, zein/SSPS NPs obtained at the same pH had much lower ζ-potential (about -2 mV) further proving that there was electrostatic interaction between SSPS and zein.					
33885154	2	61	theme	zein	256:259	arg1	nanoparticles					324:336	zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles	256:336	zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs)	256:342	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	2	61	theme	zein	256:259	arg1	stabilizers					373:383	Pickering stabilizers	363:383	Pickering stabilizers	363:383	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	2	61	theme	zein	256:259	arg1	NPs					339:341	NPs	339:341	NPs	339:341	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	9	62	theme	stability	1852:1860	arg1	properties					1792:1801	the emulsification properties	1773:1801	the emulsification properties of zein and soluble soybean polysaccharides, and stability	1773:1860	This study showed that the food-grade composite colloidal particles formed by electrostatic interaction can significantly improve the emulsification properties of zein and soluble soybean polysaccharides, and stability.					
33885154	10	63	located	observed	1897:1904	arg1	testing					1919:1925	long-term testing	1909:1925	long-term testing	1909:1925	The Pickering emulsions have been observed in long-term testing.					
33885154	10	63	located	observed	1897:1904	arg2	emulsions					1877:1885	The Pickering emulsions	1863:1885	The Pickering emulsions	1863:1885	The Pickering emulsions have been observed in long-term testing.					
33885154	7	64	located	observed	1223:1230	arg1	surface					1157:1163	the surface	1153:1163	the surface of the oil droplets	1153:1183	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	7	64	located	observed	1223:1230	arg2	layer					1213:1217	a densely packed interface layer	1186:1217	a densely packed interface layer	1186:1217	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	2	65	theme	polysaccharide	281:294	arg1	composite					314:322	soluble soybean polysaccharide (SSPS) food-grade composite	265:322	soluble soybean polysaccharide (SSPS) food-grade composite	265:322	In the current work, zein and soluble soybean polysaccharide (SSPS) food-grade composite nanoparticles (NPs) were fabricated as Pickering stabilizers.					
33885154	8	66	theme	6 mg/mL	1375:1381	arg1	concentration					1358:1370	zein concentration	1353:1370	zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL	1353:1415	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	8	67	contain	had	1449:1451	arg1	emulsions					1439:1447	the formed Pickering emulsions	1418:1447	the formed Pickering emulsions	1418:1447	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	8	67	contain	had	1449:1451	arg2	stability					1460:1468	higher stability	1453:1468	higher stability	1453:1468	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	4	68	theme	positive	695:702	arg1	ζ-potential					704:714	a positive ζ-potential	693:714	a positive ζ-potential (around at +12 mV)	693:733	At pH 4.0, the dispersions of ZPs exhibited a positive ζ-potential (around at +12 mV); however, zein/SSPS NPs obtained at the same pH had much lower ζ-potential (about -2 mV) further proving that there was electrostatic interaction between SSPS and zein.					
33885154	3	69	theme	scanning	606:613	arg1	SEM					636:638	SEM	636:638	SEM	636:638	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	3	69	theme	scanning	606:613	arg1	microscopy					624:633	scanning electron microscopy	606:633	the scanning electron microscopy (SEM) images	602:646	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	1	70	theme	"	174:174	arg1	character					176:184	their "surfactant-free" character	152:184	their "surfactant-free" character	152:184	Pickering emulsions have received wide attention due to their "surfactant-free" character and the ability of delivery bioactive compounds.					
33885154	8	71	theme	SSPS	1387:1390	arg1	concentration					1392:1404	SSPS concentration	1387:1404	SSPS concentration	1387:1404	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	7	72	theme	laser	1244:1248	arg1	CLSM					1271:1274	CLSM	1271:1274	CLSM	1271:1274	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	7	72	theme	laser	1244:1248	arg1	microscopy					1259:1268	confocal laser scanning microscopy	1235:1268	confocal laser scanning microscopy (CLSM)	1235:1275	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	3	73	from	nanoparticles	546:558	arg1	formulations					573:584	all formulations	569:584	all formulations	569:584	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	4	74	contain	had	783:785	arg1	NPs					755:757	zein/SSPS NPs	745:757	zein/SSPS NPs obtained at the same pH	745:781	At pH 4.0, the dispersions of ZPs exhibited a positive ζ-potential (around at +12 mV); however, zein/SSPS NPs obtained at the same pH had much lower ζ-potential (about -2 mV) further proving that there was electrostatic interaction between SSPS and zein.					
33885154	4	74	contain	had	783:785	arg2	ζ-potential					798:808	much lower ζ-potential	787:808	much lower ζ-potential (about -2 mV)	787:822	At pH 4.0, the dispersions of ZPs exhibited a positive ζ-potential (around at +12 mV); however, zein/SSPS NPs obtained at the same pH had much lower ζ-potential (about -2 mV) further proving that there was electrostatic interaction between SSPS and zein.					
33885154	5	75	theme	composite	908:916	arg1	nanoparticles					918:930	The composite nanoparticles	904:930	The composite nanoparticles (NPs)	904:936	The composite nanoparticles (NPs) were well dispersed through the results of polydispersity index (PDI).					
33885154	5	75	theme	composite	908:916	arg1	NPs					933:935	NPs	933:935	NPs	933:935	The composite nanoparticles (NPs) were well dispersed through the results of polydispersity index (PDI).					
33885154	6	76	theme	zein/SSPS	1050:1058	arg1	NPs					1060:1062	zein/SSPS NPs	1050:1062	zein/SSPS NPs stabilized Pickering emulsions	1050:1093	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	6	77	theme	physical	1013:1020	arg1	properties					1022:1031	physical properties	1013:1031	physical properties	1013:1031	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	8	78	theme	formed	1422:1427	arg1	emulsions					1439:1447	the formed Pickering emulsions	1418:1447	the formed Pickering emulsions	1418:1447	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	3	79	theme	zein	458:461	arg1	concentration					441:453	the concentration	437:453	the concentration of zein and SSPS	437:470	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	4	80	dep	ζ-potential	704:714	arg1	+12 mV					727:732	+12 mV	727:732	+12 mV	727:732	At pH 4.0, the dispersions of ZPs exhibited a positive ζ-potential (around at +12 mV); however, zein/SSPS NPs obtained at the same pH had much lower ζ-potential (about -2 mV) further proving that there was electrostatic interaction between SSPS and zein.					
33885154	3	81	theme	microscopy	624:633	arg1	images					641:646	the scanning electron microscopy (SEM) images	602:646	the scanning electron microscopy (SEM) images	602:646	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	0	82	theme	soybean	28:34	arg1	polysaccharide					36:49	zein/soluble soybean polysaccharide	15:49	zein/soluble soybean polysaccharide	15:49	Development of zein/soluble soybean polysaccharide nanoparticle-stabilized Pickering emulsions.					
33885154	7	83	theme	packed	1196:1201	arg1	layer					1213:1217	a densely packed interface layer	1186:1217	a densely packed interface layer	1186:1217	On the surface of the oil droplets, a densely packed interface layer was observed by confocal laser scanning microscopy (CLSM), which could prevent oil droplets from coalescence and Ostwald ripening.					
33885154	9	84	theme	composite	1681:1689	arg1	particles					1701:1709	the food-grade composite colloidal particles	1666:1709	the food-grade composite colloidal particles formed by electrostatic interaction	1666:1745	This study showed that the food-grade composite colloidal particles formed by electrostatic interaction can significantly improve the emulsification properties of zein and soluble soybean polysaccharides, and stability.					
33885154	8	85	theme	higher	1453:1458	arg1	stability					1460:1468	higher stability	1453:1468	higher stability	1453:1468	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	6	86	dep	properties	1022:1031	arg1	The					1009:1011	The	1009:1011	The	1009:1011	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	8	87	used	utilized	1537:1544	arg2	findings					1507:1514	The findings	1503:1514	The findings of this study	1503:1528	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	3	88	theme	SSPS	467:470	arg1	concentration					441:453	the concentration	437:453	the concentration of zein and SSPS	437:470	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	6	89	theme	Pickering	1075:1083	arg1	emulsions					1085:1093	Pickering emulsions	1075:1093	Pickering emulsions	1075:1093	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	3	90	theme	NPs	421:423	arg1	size					399:402	The particle size	386:402	The particle size of the composite NPs	386:423	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	8	91	theme	°C.	1476:1478	arg1	APPLICATION					1490:1500	25 °C. PRACTICAL APPLICATION	1473:1500	25 °C. PRACTICAL APPLICATION	1473:1500	At zein concentration of 6 mg/mL and SSPS concentration of 1 mg/mL, the formed Pickering emulsions had higher stability at 25 °C. PRACTICAL APPLICATION: The findings of this study can be utilized and integrated to further extend the application of zein in foods, medicine, or cosmetics field.					
33885154	3	92	theme	hydrodynamic	504:515	arg1	diameters					517:525	larger hydrodynamic diameters	497:525	larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images	497:646	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	6	93	theme	NPs	1060:1062	arg1	stability					1037:1045	stability	1037:1045	stability	1037:1045	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	6	93	theme	NPs	1060:1062	arg1	properties					1022:1031	physical properties	1013:1031	physical properties	1013:1031	The physical properties and stability of zein/SSPS NPs stabilized Pickering emulsions were evaluated at a fixed oil phase volume (30%, v/v).					
33885154	4	94	theme	zein/SSPS	745:753	arg1	NPs					755:757	zein/SSPS NPs	745:757	zein/SSPS NPs obtained at the same pH	745:781	At pH 4.0, the dispersions of ZPs exhibited a positive ζ-potential (around at +12 mV); however, zein/SSPS NPs obtained at the same pH had much lower ζ-potential (about -2 mV) further proving that there was electrostatic interaction between SSPS and zein.					
33885154	3	95	theme	zein	541:544	arg1	ZPs					561:563	ZPs	561:563	ZPs	561:563	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
33885154	3	95	theme	zein	541:544	arg1	nanoparticles					546:558	zein nanoparticles	541:558	zein nanoparticles (ZPs)	541:564	The particle size of the composite NPs varied with the concentration of zein and SSPS, consequently leading to larger hydrodynamic diameters compared with zein nanoparticles (ZPs) in all formulations, also seen from the scanning electron microscopy (SEM) images.					
32534093	2	0	dep	arabinose	250:258	arg1	%					295:295	41%	293:295	41% uronic acids	293:308	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	0	dep	arabinose	250:258	arg1	%					273:273	~24, 13 and 8%	260:273	~24, 13 and 8% respectively)	260:287	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	5	1	theme	0.3-2.5 wt	705:714	arg1	%					715:715	%	715:715	%	715:715	The polysaccharide formed a highly viscous solution in water with apparent zero shear viscosities of 0.59-772 Pa·s at concentrations 0.3-2.5 wt%.					
32534093	0	2	from	Characterization	0:15	arg1	seeds					58:62	Cola millenii seeds	44:62	Cola millenii seeds	44:62	Characterization of the polysaccharide from Cola millenii seeds.					
32534093	8	3	dep	polysaccharide	905:918	arg1	has					920:922	has	920:922	has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations	920:1034	The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.					
32534093	2	4	theme	arabinose	250:258	arg1	acid					331:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	4	theme	arabinose	250:258	arg1	sugars					211:216	59% neutral sugars	199:216	59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid]	199:335	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	4	5	theme	salt	552:555	arg1	concentration					557:569	salt concentration	552:569	salt concentration	552:569	The polysaccharide exhibited polyelectrolyte properties with the intrinsic viscosity varying with salt concentration.					
32534093	5	6	theme	%	715:715	arg1	concentrations					690:703	concentrations	690:703	concentrations 0.3-2.5 wt%	690:715	The polysaccharide formed a highly viscous solution in water with apparent zero shear viscosities of 0.59-772 Pa·s at concentrations 0.3-2.5 wt%.					
32534093	0	7	from	seeds	58:62	arg1	Characterization					0:15	Characterization	0:15	Characterization of the polysaccharide from Cola millenii seeds.	0:63	Characterization of the polysaccharide from Cola millenii seeds.					
32534093	0	7	from	seeds	58:62	arg1	polysaccharide					24:37	the polysaccharide	20:37	the polysaccharide from Cola millenii seeds	20:62	Characterization of the polysaccharide from Cola millenii seeds.					
32534093	5	8	with	solution	615:622	arg1	viscosities					658:668	apparent zero shear viscosities	638:668	apparent zero shear viscosities of 0.59-772 Pa·s	638:685	The polysaccharide formed a highly viscous solution in water with apparent zero shear viscosities of 0.59-772 Pa·s at concentrations 0.3-2.5 wt%.					
32534093	8	9	from	agent	980:984	arg1	food					989:992	food	989:992	food	989:992	The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.					
32534093	8	9	from	agent	980:984	arg1	cosmetic					1014:1021	cosmetic	1014:1021	cosmetic	1014:1021	The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.					
32534093	8	9	from	agent	980:984	arg1	pharmaceutical					995:1008	pharmaceutical	995:1008	pharmaceutical	995:1008	The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.					
32534093	1	10	from	seeds	150:154	arg1	polysaccharide					116:129	a water soluble polysaccharide	100:129	a water soluble polysaccharide from Cola millenii seeds	100:154	We have isolated and characterized a water soluble polysaccharide from Cola millenii seeds.					
32534093	3	11	theme	average	360:366	arg1	4.7 × 106 g/mol					403:417	4.7 × 106 g/mol	403:417	4.7 × 106 g/mol	403:417	The weight and number average molar mass values were found to be 4.7 × 106 g/mol and 3.5 × 106 g/mol, respectively.					
32534093	3	11	theme	average	360:366	arg1	values					379:384	The weight and number average molar mass values	338:384	The weight and number average molar mass values	338:384	The weight and number average molar mass values were found to be 4.7 × 106 g/mol and 3.5 × 106 g/mol, respectively.					
32534093	6	12	theme	low	765:767	arg1	concentrations					769:782	very low concentrations	760:782	very low concentrations	760:782	The solutions were shear thinning even at very low concentrations.					
32534093	2	13	dep	%	295:295	arg1	acids					304:308	uronic acids	297:308	41% uronic acids	293:308	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	14	theme	galacturonic	318:329	arg1	acid					331:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	14	theme	galacturonic	318:329	arg1	sugars					211:216	59% neutral sugars	199:216	59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid]	199:335	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	8	15	theme	rheological	870:880	arg1	behavior					882:889	The rheological behavior	866:889	The rheological behavior	866:889	The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.					
32534093	2	16	theme	[mainly	310:316	arg1	acid					331:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	16	theme	[mainly	310:316	arg1	sugars					211:216	59% neutral sugars	199:216	59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid]	199:335	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	5	17	theme	viscous	607:613	arg1	solution					615:622	a highly viscous solution	598:622	a highly viscous solution in water with apparent zero shear viscosities of 0.59-772 Pa·s	598:685	The polysaccharide formed a highly viscous solution in water with apparent zero shear viscosities of 0.59-772 Pa·s at concentrations 0.3-2.5 wt%.					
32534093	5	18	from	solution	615:622	arg1	water					627:631	water	627:631	water	627:631	The polysaccharide formed a highly viscous solution in water with apparent zero shear viscosities of 0.59-772 Pa·s at concentrations 0.3-2.5 wt%.					
32534093	7	19	theme	gel-like	815:822	arg1	characteristics					824:838	gel-like characteristics	815:838	gel-like characteristics	815:838	The mechanical spectra showed gel-like characteristics at concentrations >2 wt%.					
32534093	3	20	theme	number	353:358	arg1	4.7 × 106 g/mol					403:417	4.7 × 106 g/mol	403:417	4.7 × 106 g/mol	403:417	The weight and number average molar mass values were found to be 4.7 × 106 g/mol and 3.5 × 106 g/mol, respectively.					
32534093	3	20	theme	number	353:358	arg1	values					379:384	The weight and number average molar mass values	338:384	The weight and number average molar mass values	338:384	The weight and number average molar mass values were found to be 4.7 × 106 g/mol and 3.5 × 106 g/mol, respectively.					
32534093	0	21	theme	polysaccharide	24:37	arg1	Characterization					0:15	Characterization	0:15	Characterization of the polysaccharide from Cola millenii seeds.	0:63	Characterization of the polysaccharide from Cola millenii seeds.					
32534093	7	22	theme	mechanical	789:798	arg1	spectra					800:806	The mechanical spectra	785:806	The mechanical spectra	785:806	The mechanical spectra showed gel-like characteristics at concentrations >2 wt%.					
32534093	8	23	dep	food	989:992	arg1	formulations					1023:1034	formulations	1023:1034	formulations	1023:1034	The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.					
32534093	4	24	theme	intrinsic	519:527	arg1	viscosity					529:537	the intrinsic viscosity	515:537	the intrinsic viscosity varying with salt concentration	515:569	The polysaccharide exhibited polyelectrolyte properties with the intrinsic viscosity varying with salt concentration.					
32534093	5	25	theme	0.59-772 Pa·s	673:685	arg1	viscosities					658:668	apparent zero shear viscosities	638:668	apparent zero shear viscosities of 0.59-772 Pa·s	638:685	The polysaccharide formed a highly viscous solution in water with apparent zero shear viscosities of 0.59-772 Pa·s at concentrations 0.3-2.5 wt%.					
32534093	1	26	theme	millenii	141:148	arg1	seeds					150:154	Cola millenii seeds	136:154	Cola millenii seeds	136:154	We have isolated and characterized a water soluble polysaccharide from Cola millenii seeds.					
32534093	0	27	theme	Cola	44:47	arg1	seeds					58:62	Cola millenii seeds	44:62	Cola millenii seeds	44:62	Characterization of the polysaccharide from Cola millenii seeds.					
32534093	2	28	theme	neutral	203:209	arg1	acid					331:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	28	theme	neutral	203:209	arg1	galactose					236:244	galactose	236:244	galactose	236:244	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	28	theme	neutral	203:209	arg1	sugars					211:216	59% neutral sugars	199:216	59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid]	199:335	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	28	theme	neutral	203:209	arg1	rhamnose					226:233	rhamnose	226:233	rhamnose	226:233	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	3	29	theme	mass	374:377	arg1	4.7 × 106 g/mol					403:417	4.7 × 106 g/mol	403:417	4.7 × 106 g/mol	403:417	The weight and number average molar mass values were found to be 4.7 × 106 g/mol and 3.5 × 106 g/mol, respectively.					
32534093	3	29	theme	mass	374:377	arg1	values					379:384	The weight and number average molar mass values	338:384	The weight and number average molar mass values	338:384	The weight and number average molar mass values were found to be 4.7 × 106 g/mol and 3.5 × 106 g/mol, respectively.					
32534093	3	30	theme	weight	342:347	arg1	4.7 × 106 g/mol					403:417	4.7 × 106 g/mol	403:417	4.7 × 106 g/mol	403:417	The weight and number average molar mass values were found to be 4.7 × 106 g/mol and 3.5 × 106 g/mol, respectively.					
32534093	3	30	theme	weight	342:347	arg1	values					379:384	The weight and number average molar mass values	338:384	The weight and number average molar mass values	338:384	The weight and number average molar mass values were found to be 4.7 × 106 g/mol and 3.5 × 106 g/mol, respectively.					
32534093	2	31	theme	%	201:201	arg1	acid					331:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	31	theme	%	201:201	arg1	galactose					236:244	galactose	236:244	galactose	236:244	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	31	theme	%	201:201	arg1	sugars					211:216	59% neutral sugars	199:216	59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid]	199:335	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	31	theme	%	201:201	arg1	rhamnose					226:233	rhamnose	226:233	rhamnose	226:233	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	5	32	theme	apparent	638:645	arg1	viscosities					658:668	apparent zero shear viscosities	638:668	apparent zero shear viscosities of 0.59-772 Pa·s	638:685	The polysaccharide formed a highly viscous solution in water with apparent zero shear viscosities of 0.59-772 Pa·s at concentrations 0.3-2.5 wt%.					
32534093	2	33	theme	59	199:200	arg1	%					201:201	%	201:201	%	201:201	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	6	34	theme	shear	737:741	arg1	solutions					722:730	The solutions	718:730	The solutions	718:730	The solutions were shear thinning even at very low concentrations.					
32534093	6	34	theme	shear	737:741	arg1	thinning					743:750	shear thinning	737:750	shear thinning	737:750	The solutions were shear thinning even at very low concentrations.					
32534093	1	35	theme	soluble	108:114	arg1	polysaccharide					116:129	a water soluble polysaccharide	100:129	a water soluble polysaccharide from Cola millenii seeds	100:154	We have isolated and characterized a water soluble polysaccharide from Cola millenii seeds.					
32534093	0	36	theme	millenii	49:56	arg1	seeds					58:62	Cola millenii seeds	44:62	Cola millenii seeds	44:62	Characterization of the polysaccharide from Cola millenii seeds.					
32534093	4	37	theme	polyelectrolyte	483:497	arg1	properties					499:508	polyelectrolyte properties	483:508	polyelectrolyte properties	483:508	The polysaccharide exhibited polyelectrolyte properties with the intrinsic viscosity varying with salt concentration.					
32534093	5	38	theme	shear	652:656	arg1	viscosities					658:668	apparent zero shear viscosities	638:668	apparent zero shear viscosities of 0.59-772 Pa·s	638:685	The polysaccharide formed a highly viscous solution in water with apparent zero shear viscosities of 0.59-772 Pa·s at concentrations 0.3-2.5 wt%.					
32534093	8	39	theme	thickener	955:963	arg1	agent					980:984	a thickener and suspending agent	953:984	a thickener and suspending agent in food, pharmaceutical and cosmetic formulations	953:1034	The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.					
32534093	8	39	theme	thickener	955:963	arg1	potential					924:932	potential	924:932	potential for application	924:948	The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.					
32534093	1	40	theme	Cola	136:139	arg1	seeds					150:154	Cola millenii seeds	136:154	Cola millenii seeds	136:154	We have isolated and characterized a water soluble polysaccharide from Cola millenii seeds.					
32534093	3	41	theme	molar	368:372	arg1	4.7 × 106 g/mol					403:417	4.7 × 106 g/mol	403:417	4.7 × 106 g/mol	403:417	The weight and number average molar mass values were found to be 4.7 × 106 g/mol and 3.5 × 106 g/mol, respectively.					
32534093	3	41	theme	molar	368:372	arg1	values					379:384	The weight and number average molar mass values	338:384	The weight and number average molar mass values	338:384	The weight and number average molar mass values were found to be 4.7 × 106 g/mol and 3.5 × 106 g/mol, respectively.					
32534093	2	42	theme	uronic	297:302	arg1	acids					304:308	uronic acids	297:308	41% uronic acids	293:308	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	43	dep	sugars	211:216	arg1	acid					331:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid	250:334	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	43	dep	sugars	211:216	arg1	galactose					236:244	galactose	236:244	galactose	236:244	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	43	dep	sugars	211:216	arg1	sugars					211:216	59% neutral sugars	199:216	59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid]	199:335	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	2	43	dep	sugars	211:216	arg1	rhamnose					226:233	rhamnose	226:233	rhamnose	226:233	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	6	44	from	concentrations	769:782	arg1	solutions					722:730	The solutions	718:730	The solutions	718:730	The solutions were shear thinning even at very low concentrations.					
32534093	6	44	from	concentrations	769:782	arg1	thinning					743:750	shear thinning	737:750	shear thinning	737:750	The solutions were shear thinning even at very low concentrations.					
32534093	2	45	theme	sugars	211:216	arg1	total					190:194	a total	188:194	a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid]	188:335	It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid].					
32534093	8	46	theme	suspending	969:978	arg1	agent					980:984	a thickener and suspending agent	953:984	a thickener and suspending agent in food, pharmaceutical and cosmetic formulations	953:1034	The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.					
32534093	8	46	theme	suspending	969:978	arg1	potential					924:932	potential	924:932	potential for application	924:948	The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.					
32265863	9	0	theme	glycosylation	1653:1665	arg1	loci					1667:1670	flagellar glycosylation loci	1643:1670	flagellar glycosylation loci	1643:1670	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	1	1	gly	glycosylated	197:208	arg1	structures					218:227	glycosylated surface structures	197:227	glycosylated surface structures that contribute to virulence	197:256	Many bacterial pathogens display glycosylated surface structures that contribute to virulence, and targeting these structures is a viable strategy for pathogen control.					
32265863	10	2	theme	glycan	1772:1777	arg1	diversity					1779:1787	flagellar glycan diversity	1762:1787	flagellar glycan diversity	1762:1787	Our results point toward flagellar glycan diversity as the mechanism of resistance to FlaGrab.					
32265863	9	3	theme	C.	1679:1680	arg1	strains					1689:1695	two C. jejuni strains	1675:1695	two C. jejuni strains naturally resistant to FlaGrab binding	1675:1734	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	2	4	theme	glycans	417:423	arg1	diversity					394:402	a vast diversity	387:402	a vast diversity of flagellar glycans	387:423	The foodborne pathogen Campylobacter jejuni expresses a vast diversity of flagellar glycans, and flagellar glycosylation is essential for its virulence.					
32265863	12	5	theme	phage	2158:2162	arg1	proteins					2164:2171	phage proteins	2158:2171	phage proteins	2158:2171	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	3	6	theme	flagellar	552:560	arg1	glycans					562:568	flagellar glycans	552:568	flagellar glycans	552:568	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	9	7	theme	strains	1689:1695	arg1	glycans					1631:1637	the flagellar glycans	1617:1637	the flagellar glycans	1617:1637	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	9	7	theme	strains	1689:1695	arg1	loci					1667:1670	flagellar glycosylation loci	1643:1670	flagellar glycosylation loci	1643:1670	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	1	8	theme	surface	210:216	arg1	structures					218:227	glycosylated surface structures	197:227	glycosylated surface structures that contribute to virulence	197:256	Many bacterial pathogens display glycosylated surface structures that contribute to virulence, and targeting these structures is a viable strategy for pathogen control.					
32265863	9	9	theme	resistant	1707:1715	arg1	strains					1689:1695	two C. jejuni strains	1675:1695	two C. jejuni strains naturally resistant to FlaGrab binding	1675:1734	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	4	10	theme	flagellar	756:764	arg1	glycans					766:772	host flagellar glycans	751:772	host flagellar glycans	751:772	However, interactions between Campylobacter phages and host flagellar glycans have not been characterized in detail.					
32265863	5	11	theme	renamed	853:859	arg1	FlaGrab					861:867	now renamed FlaGrab	849:867	now renamed FlaGrab	849:867	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	5	11	theme	renamed	853:859	arg1	Gp047					842:846	Gp047	842:846	Gp047 (now renamed FlaGrab)	842:868	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	9	12	theme	FlaGrab	1720:1726	arg1	binding					1728:1734	FlaGrab binding	1720:1734	FlaGrab binding	1720:1734	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	7	13	theme	motile	1388:1393	arg1	flagella					1395:1402	motile flagella	1388:1402	motile flagella	1388:1402	Here we use RNA-Seq to show that FlaGrab exposure leads C. jejuni 11168 cells to downregulate expression of energy metabolism genes, and that FlaGrab-induced growth inhibition is dependent on motile flagella.					
32265863	0	14	theme	Specific	136:143	arg1	Glycans					155:161	Specific Flagellar Glycans	136:161	Specific Flagellar Glycans	136:161	Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans.					
32265863	10	15	theme	resistance	1809:1818	arg1	mechanism					1796:1804	the mechanism	1792:1804	the mechanism of resistance to FlaGrab	1792:1829	Our results point toward flagellar glycan diversity as the mechanism of resistance to FlaGrab.					
32265863	5	16	theme	C.	921:922	arg1	jejuni					924:929	C. jejuni	921:929	C. jejuni	921:929	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	12	17	theme	glycan	2127:2132	arg1	diversity					2134:2142	flagellar glycan diversity	2117:2142	flagellar glycan diversity	2117:2142	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	5	18	theme	nine-carbon	955:965	arg1	acid					1008:1011	the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid	951:1011	the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid	951:1011	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	9	19	from	resistance	1575:1584	arg1	jejuni					1592:1597	C. jejuni	1589:1597	C. jejuni	1589:1597	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	4	20	theme	host	751:754	arg1	glycans					766:772	host flagellar glycans	751:772	host flagellar glycans	751:772	However, interactions between Campylobacter phages and host flagellar glycans have not been characterized in detail.					
32265863	7	21	theme	FlaGrab-induced	1338:1352	arg1	inhibition					1361:1370	FlaGrab-induced growth inhibition	1338:1370	FlaGrab-induced growth inhibition	1338:1370	Here we use RNA-Seq to show that FlaGrab exposure leads C. jejuni 11168 cells to downregulate expression of energy metabolism genes, and that FlaGrab-induced growth inhibition is dependent on motile flagella.					
32265863	12	22	theme	as-yet	2071:2076	arg1	mechanisms					2091:2100	as-yet unidentified mechanisms	2071:2100	as-yet unidentified mechanisms for generating flagellar glycan diversity	2071:2142	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	12	22	theme	as-yet	2071:2076	arg1	jejuni					2056:2061	C. jejuni	2053:2061	C. jejuni	2053:2061	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	7	23	theme	FlaGrab	1229:1235	arg1	exposure					1237:1244	FlaGrab exposure	1229:1244	FlaGrab exposure	1229:1244	Here we use RNA-Seq to show that FlaGrab exposure leads C. jejuni 11168 cells to downregulate expression of energy metabolism genes, and that FlaGrab-induced growth inhibition is dependent on motile flagella.					
32265863	1	24	theme	bacterial	169:177	arg1	pathogens					179:187	Many bacterial pathogens	164:187	Many bacterial pathogens	164:187	Many bacterial pathogens display glycosylated surface structures that contribute to virulence, and targeting these structures is a viable strategy for pathogen control.					
32265863	8	25	theme	cell	1528:1531	arg1	growth					1533:1538	retarded cell growth	1519:1538	retarded cell growth	1519:1538	Our results are consistent with a model whereby FlaGrab binding transmits a signal through flagella that leads to retarded cell growth.					
32265863	3	26	from	bacteriophages	632:645	arg1	pressure					618:625	evolutionary pressure	605:625	evolutionary pressure from bacteriophages (phages)	605:654	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	9	27	theme	FlaGrab	1567:1573	arg1	resistance					1575:1584	FlaGrab resistance	1567:1584	FlaGrab resistance in C. jejuni	1567:1597	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	6	28	theme	growth	1099:1104	arg1	inhibition					1106:1115	this growth inhibition	1094:1115	this growth inhibition	1094:1115	However, the mechanism of this growth inhibition, as well as how C. jejuni might resist this activity, are not well-understood.					
32265863	11	29	theme	glycan-binding	1924:1937	arg1	protein					1939:1945	this phage-encoded flagellar glycan-binding protein	1895:1945	this phage-encoded flagellar glycan-binding protein	1895:1945	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
32265863	1	30	theme	viable	295:300	arg1	strategy					302:309	a viable strategy	293:309	a viable strategy for pathogen control	293:330	Many bacterial pathogens display glycosylated surface structures that contribute to virulence, and targeting these structures is a viable strategy for pathogen control.					
32265863	11	31	from	interaction	1875:1885	arg1	terms					1970:1974	terms	1970:1974	terms of mechanism of action and mechanism of resistance	1970:2025	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
32265863	0	32	theme	Phage-Encoded	11:23	arg1	FlaGrab					25:31	Phage-Encoded FlaGrab	11:31	Phage-Encoded FlaGrab	11:31	Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans.					
32265863	11	33	theme	resistance	2016:2025	arg1	mechanism					2003:2011	mechanism	2003:2011	mechanism	2003:2011	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
32265863	11	33	theme	resistance	2016:2025	arg1	action					1992:1997	action	1992:1997	action	1992:1997	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
32265863	5	34	theme	monosaccharide	967:980	arg1	acid					1008:1011	the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid	951:1011	the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid	951:1011	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	7	35	theme	metabolism	1311:1320	arg1	genes					1322:1326	energy metabolism genes	1304:1326	energy metabolism genes	1304:1326	Here we use RNA-Seq to show that FlaGrab exposure leads C. jejuni 11168 cells to downregulate expression of energy metabolism genes, and that FlaGrab-induced growth inhibition is dependent on motile flagella.					
32265863	11	36	dep	protein	1939:1945	arg1	both					1962:1965	both	1962:1965	both	1962:1965	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
32265863	12	37	theme	surface	2225:2231	arg1	glycans					2233:2239	bacterial surface glycans	2215:2239	bacterial surface glycans	2215:2239	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	0	38	theme	Campylobacter	43:55	arg1	jejuni					57:62	Motile Campylobacter jejuni	36:62	Motile Campylobacter jejuni	36:62	Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans.					
32265863	5	39	theme	cell	1055:1058	arg1	growth					1060:1065	cell growth	1055:1065	cell growth	1055:1065	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	2	40	theme	Campylobacter	356:368	arg1	jejuni					370:375	Campylobacter jejuni	356:375	The foodborne pathogen Campylobacter jejuni	333:375	The foodborne pathogen Campylobacter jejuni expresses a vast diversity of flagellar glycans, and flagellar glycosylation is essential for its virulence.					
32265863	11	41	theme	phage-encoded	1900:1912	arg1	protein					1939:1945	this phage-encoded flagellar glycan-binding protein	1895:1945	this phage-encoded flagellar glycan-binding protein	1895:1945	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
32265863	9	42	theme	flagellar	1643:1651	arg1	loci					1667:1670	flagellar glycosylation loci	1643:1670	flagellar glycosylation loci	1643:1670	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	7	43	theme	jejuni	1255:1260	arg1	11168					1262:1266	C. jejuni 11168	1252:1266	C. jejuni 11168	1252:1266	Here we use RNA-Seq to show that FlaGrab exposure leads C. jejuni 11168 cells to downregulate expression of energy metabolism genes, and that FlaGrab-induced growth inhibition is dependent on motile flagella.					
32265863	2	44	theme	vast	389:392	arg1	diversity					394:402	a vast diversity	387:402	a vast diversity of flagellar glycans	387:423	The foodborne pathogen Campylobacter jejuni expresses a vast diversity of flagellar glycans, and flagellar glycosylation is essential for its virulence.					
32265863	12	45	theme	C.	2053:2054	arg1	mechanisms					2091:2100	as-yet unidentified mechanisms	2071:2100	as-yet unidentified mechanisms for generating flagellar glycan diversity	2071:2142	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	12	45	theme	C.	2053:2054	arg1	jejuni					2056:2061	C. jejuni	2053:2061	C. jejuni	2053:2061	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	3	46	theme	glycans	562:568	arg1	glycans					562:568	flagellar glycans	552:568	flagellar glycans	552:568	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	3	46	theme	glycans	562:568	arg1	jejuni					515:520	C. jejuni	512:520	C. jejuni	512:520	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	3	46	theme	glycans	562:568	arg1	set					545:547	such a diverse set	530:547	such a diverse set of flagellar glycans	530:568	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	10	47	theme	flagellar	1762:1770	arg1	diversity					1779:1787	flagellar glycan diversity	1762:1787	flagellar glycan diversity	1762:1787	Our results point toward flagellar glycan diversity as the mechanism of resistance to FlaGrab.					
32265863	5	48	theme	conserved	873:881	arg1	Gp047					842:846	Gp047	842:846	Gp047 (now renamed FlaGrab)	842:868	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	5	48	theme	conserved	873:881	arg1	protein					903:909	a conserved Campylobacter phage protein	871:909	a conserved Campylobacter phage protein	871:909	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	9	49	theme	jejuni	1682:1687	arg1	strains					1689:1695	two C. jejuni strains	1675:1695	two C. jejuni strains naturally resistant to FlaGrab binding	1675:1734	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	2	50	theme	foodborne	337:345	arg1	pathogen					347:354	The foodborne pathogen Campylobacter jejuni	333:375	The foodborne pathogen Campylobacter jejuni	333:375	The foodborne pathogen Campylobacter jejuni expresses a vast diversity of flagellar glycans, and flagellar glycosylation is essential for its virulence.					
32265863	1	51	theme	glycosylated	197:208	arg1	structures					218:227	glycosylated surface structures	197:227	glycosylated surface structures that contribute to virulence	197:256	Many bacterial pathogens display glycosylated surface structures that contribute to virulence, and targeting these structures is a viable strategy for pathogen control.					
32265863	8	52	theme	FlaGrab	1453:1459	arg1	binding					1461:1467	FlaGrab binding	1453:1467	FlaGrab binding	1453:1467	Our results are consistent with a model whereby FlaGrab binding transmits a signal through flagella that leads to retarded cell growth.					
32265863	6	53	theme	resist	1149:1154	arg1	mechanism					1081:1089	the mechanism	1077:1089	the mechanism of this growth inhibition, as well as how C. jejuni might resist this activity,	1077:1169	However, the mechanism of this growth inhibition, as well as how C. jejuni might resist this activity, are not well-understood.					
32265863	6	53	theme	resist	1149:1154	arg1	well-understood					1179:1193	well-understood	1179:1193	well-understood	1179:1193	However, the mechanism of this growth inhibition, as well as how C. jejuni might resist this activity, are not well-understood.					
32265863	0	54	theme	Flagellar	145:153	arg1	Glycans					155:161	Specific Flagellar Glycans	136:161	Specific Flagellar Glycans	136:161	Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans.					
32265863	3	55	theme	evolutionary	605:616	arg1	pressure					618:625	evolutionary pressure	605:625	evolutionary pressure from bacteriophages (phages)	605:654	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	5	56	theme	Campylobacter	883:895	arg1	Gp047					842:846	Gp047	842:846	Gp047 (now renamed FlaGrab)	842:868	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	5	56	theme	Campylobacter	883:895	arg1	protein					903:909	a conserved Campylobacter phage protein	871:909	a conserved Campylobacter phage protein	871:909	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	4	57	theme	Campylobacter	726:738	arg1	phages					740:745	Campylobacter phages	726:745	Campylobacter phages	726:745	However, interactions between Campylobacter phages and host flagellar glycans have not been characterized in detail.					
32265863	8	58	with	consistent	1421:1430	arg1	model					1439:1443	a model	1437:1443	a model whereby FlaGrab binding transmits a signal through flagella that leads to retarded cell growth	1437:1538	Our results are consistent with a model whereby FlaGrab binding transmits a signal through flagella that leads to retarded cell growth.					
32265863	11	59	theme	mechanism	2003:2011	arg1	mechanism					1979:1987	mechanism	1979:1987	mechanism of action and mechanism of resistance	1979:2025	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
32265863	9	60	from	jejuni	1592:1597	arg1	mechanisms					1553:1562	mechanisms	1553:1562	mechanisms of FlaGrab resistance in C. jejuni	1553:1597	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	12	61	theme	flagellar	2117:2125	arg1	diversity					2134:2142	flagellar glycan diversity	2117:2142	flagellar glycan diversity	2117:2142	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	11	62	theme	action	1992:1997	arg1	mechanism					1979:1987	mechanism	1979:1987	mechanism of action and mechanism of resistance	1979:2025	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
32265863	5	63	theme	phage	897:901	arg1	Gp047					842:846	Gp047	842:846	Gp047 (now renamed FlaGrab)	842:868	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	5	63	theme	phage	897:901	arg1	protein					903:909	a conserved Campylobacter phage protein	871:909	a conserved Campylobacter phage protein	871:909	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	7	64	theme	growth	1354:1359	arg1	inhibition					1361:1370	FlaGrab-induced growth inhibition	1338:1370	FlaGrab-induced growth inhibition	1338:1370	Here we use RNA-Seq to show that FlaGrab exposure leads C. jejuni 11168 cells to downregulate expression of energy metabolism genes, and that FlaGrab-induced growth inhibition is dependent on motile flagella.					
32265863	1	65	theme	Many	164:167	arg1	pathogens					179:187	Many bacterial pathogens	164:187	Many bacterial pathogens	164:187	Many bacterial pathogens display glycosylated surface structures that contribute to virulence, and targeting these structures is a viable strategy for pathogen control.					
32265863	8	66	theme	retarded	1519:1526	arg1	growth					1533:1538	retarded cell growth	1519:1538	retarded cell growth	1519:1538	Our results are consistent with a model whereby FlaGrab binding transmits a signal through flagella that leads to retarded cell growth.					
32265863	12	67	theme	unidentified	2078:2089	arg1	mechanisms					2091:2100	as-yet unidentified mechanisms	2071:2100	as-yet unidentified mechanisms for generating flagellar glycan diversity	2071:2142	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	12	67	theme	unidentified	2078:2089	arg1	jejuni					2056:2061	C. jejuni	2053:2061	C. jejuni	2053:2061	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	0	68	theme	Energy	104:109	arg1	Metabolism					111:120	Energy Metabolism	104:120	Energy Metabolism	104:120	Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans.					
32265863	5	69	theme	7-acetamidino-pseudaminic	982:1006	arg1	acid					1008:1011	the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid	951:1011	the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid	951:1011	Previously, we observed that Gp047 (now renamed FlaGrab), a conserved Campylobacter phage protein, binds to C. jejuni flagella displaying the nine-carbon monosaccharide 7-acetamidino-pseudaminic acid, and that this binding partially inhibits cell growth.					
32265863	9	70	from	mechanisms	1553:1562	arg1	jejuni					1592:1597	C. jejuni	1589:1597	C. jejuni	1589:1597	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	6	71	theme	inhibition	1106:1115	arg1	mechanism					1081:1089	the mechanism	1077:1089	the mechanism of this growth inhibition, as well as how C. jejuni might resist this activity,	1077:1169	However, the mechanism of this growth inhibition, as well as how C. jejuni might resist this activity, are not well-understood.					
32265863	6	71	theme	inhibition	1106:1115	arg1	well-understood					1179:1193	well-understood	1179:1193	well-understood	1179:1193	However, the mechanism of this growth inhibition, as well as how C. jejuni might resist this activity, are not well-understood.					
32265863	11	72	theme	C.	1951:1952	arg1	jejuni					1954:1959	C. jejuni	1951:1959	C. jejuni	1951:1959	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
32265863	9	73	theme	resistance	1575:1584	arg1	mechanisms					1553:1562	mechanisms	1553:1562	mechanisms of FlaGrab resistance in C. jejuni	1553:1597	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	7	74	theme	genes	1322:1326	arg1	expression					1290:1299	expression	1290:1299	expression of energy metabolism genes	1290:1326	Here we use RNA-Seq to show that FlaGrab exposure leads C. jejuni 11168 cells to downregulate expression of energy metabolism genes, and that FlaGrab-induced growth inhibition is dependent on motile flagella.					
32265863	2	75	dep	pathogen	347:354	arg1	jejuni					370:375	Campylobacter jejuni	356:375	The foodborne pathogen Campylobacter jejuni	333:375	The foodborne pathogen Campylobacter jejuni expresses a vast diversity of flagellar glycans, and flagellar glycosylation is essential for its virulence.					
32265863	2	76	theme	flagellar	407:415	arg1	glycans					417:423	flagellar glycans	407:423	flagellar glycans	407:423	The foodborne pathogen Campylobacter jejuni expresses a vast diversity of flagellar glycans, and flagellar glycosylation is essential for its virulence.					
32265863	0	77	theme	FlaGrab	25:31	arg1	Binding					0:6	Binding	0:6	Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella	0:71	Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans.					
32265863	9	78	theme	C.	1589:1590	arg1	jejuni					1592:1597	C. jejuni	1589:1597	C. jejuni	1589:1597	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	11	79	theme	mechanism	1979:1987	arg1	terms					1970:1974	terms	1970:1974	terms of mechanism of action and mechanism of resistance	1970:2025	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
32265863	7	80	theme	energy	1304:1309	arg1	genes					1322:1326	energy metabolism genes	1304:1326	energy metabolism genes	1304:1326	Here we use RNA-Seq to show that FlaGrab exposure leads C. jejuni 11168 cells to downregulate expression of energy metabolism genes, and that FlaGrab-induced growth inhibition is dependent on motile flagella.					
32265863	3	81	theme	diverse	537:543	arg1	glycans					562:568	flagellar glycans	552:568	flagellar glycans	552:568	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	3	81	theme	diverse	537:543	arg1	jejuni					515:520	C. jejuni	512:520	C. jejuni	512:520	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	3	81	theme	diverse	537:543	arg1	set					545:547	such a diverse set	530:547	such a diverse set of flagellar glycans	530:568	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	0	82	theme	Motile	36:41	arg1	jejuni					57:62	Motile Campylobacter jejuni	36:62	Motile Campylobacter jejuni	36:62	Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans.					
32265863	1	83	theme	pathogen	315:322	arg1	control					324:330	pathogen control	315:330	pathogen control	315:330	Many bacterial pathogens display glycosylated surface structures that contribute to virulence, and targeting these structures is a viable strategy for pathogen control.					
32265863	0	84	dep	jejuni	57:62	arg1	Flagella					64:71	Flagella	64:71	Flagella	64:71	Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans.					
32265863	2	85	theme	flagellar	430:438	arg1	glycosylation					440:452	flagellar glycosylation	430:452	flagellar glycosylation	430:452	The foodborne pathogen Campylobacter jejuni expresses a vast diversity of flagellar glycans, and flagellar glycosylation is essential for its virulence.					
32265863	12	86	theme	bacterial	2215:2223	arg1	glycans					2233:2239	bacterial surface glycans	2215:2239	bacterial surface glycans	2215:2239	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	9	87	theme	flagellar	1621:1629	arg1	glycans					1631:1637	the flagellar glycans	1617:1637	the flagellar glycans	1617:1637	To evaluate mechanisms of FlaGrab resistance in C. jejuni, we characterized the flagellar glycans and flagellar glycosylation loci of two C. jejuni strains naturally resistant to FlaGrab binding.					
32265863	6	88	theme	C.	1133:1134	arg1	jejuni					1136:1141	C. jejuni	1133:1141	C. jejuni	1133:1141	However, the mechanism of this growth inhibition, as well as how C. jejuni might resist this activity, are not well-understood.					
32265863	12	89	theme	exciting	2176:2183	arg1	lenses					2185:2190	exciting lenses	2176:2190	exciting lenses through which to study bacterial surface glycans	2176:2239	Our results suggest that C. jejuni encodes as-yet unidentified mechanisms for generating flagellar glycan diversity, and point to phage proteins as exciting lenses through which to study bacterial surface glycans.					
32265863	3	90	theme	C.	512:513	arg1	jejuni					515:520	C. jejuni	512:520	C. jejuni	512:520	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	3	90	theme	C.	512:513	arg1	glycans					562:568	flagellar glycans	552:568	flagellar glycans	552:568	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	3	90	theme	C.	512:513	arg1	set					545:547	such a diverse set	530:547	such a diverse set of flagellar glycans	530:568	Little is known about why C. jejuni encodes such a diverse set of flagellar glycans, but it has been hypothesized that evolutionary pressure from bacteriophages (phages) may have contributed to this diversity.					
32265863	7	91	theme	C.	1252:1253	arg1	11168					1262:1266	C. jejuni 11168	1252:1266	C. jejuni 11168	1252:1266	Here we use RNA-Seq to show that FlaGrab exposure leads C. jejuni 11168 cells to downregulate expression of energy metabolism genes, and that FlaGrab-induced growth inhibition is dependent on motile flagella.					
32265863	11	92	theme	flagellar	1914:1922	arg1	protein					1939:1945	this phage-encoded flagellar glycan-binding protein	1895:1945	this phage-encoded flagellar glycan-binding protein	1895:1945	Overall, we have further characterized the interaction between this phage-encoded flagellar glycan-binding protein and C. jejuni, both in terms of mechanism of action and mechanism of resistance.					
33593569	5	0	with	consistent	877:886	arg1	degradation					926:936	the corresponding polysaccharide degradation	893:936	the corresponding polysaccharide degradation	893:936	Microbial community dynamics for each substrate were consistent with the corresponding polysaccharide degradation.					
33593569	4	1	theme	acid	746:749	arg1	production					751:760	short chain fatty acid production	728:760	short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG	728:821	This combination explains the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG.					
33593569	1	2	theme	human	303:307	arg1	inoculum					316:323	a human faecal inoculum	301:323	a human faecal inoculum	301:323	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	2	3	theme	fermentation	507:518	arg1	rate					520:523	fermentation rate	507:523	fermentation rate of XG	507:529	Compared to individual polysaccharides, the fermentation extent of BC and fermentation rate of XG were promoted in BC&XG.					
33593569	4	4	theme	production	751:760	arg1	amount					718:723	the similar amount	706:723	the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG	706:821	This combination explains the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG.					
33593569	4	4	theme	production	751:760	arg1	production					751:760	short chain fatty acid production	728:760	short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG	728:821	This combination explains the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG.					
33593569	1	5	theme	faecal	309:314	arg1	inoculum					316:323	a human faecal inoculum	301:323	a human faecal inoculum	301:323	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	3	6	from	fermented	655:663	arg1	BC&XG					673:677	BC&XG	673:677	BC&XG	673:677	XG embedded in the BCXG composite was degraded less than in BC&XG, while more cellulose in BCXG was fermented than in BC&XG.					
33593569	2	7	theme	BC	500:501	arg1	rate					520:523	fermentation rate	507:523	fermentation rate of XG	507:529	Compared to individual polysaccharides, the fermentation extent of BC and fermentation rate of XG were promoted in BC&XG.					
33593569	2	7	theme	BC	500:501	arg1	extent					490:495	the fermentation extent	473:495	the fermentation extent of BC	473:501	Compared to individual polysaccharides, the fermentation extent of BC and fermentation rate of XG were promoted in BC&XG.					
33593569	1	8	theme	in	167:168	arg1	fermentation					176:187	in vitro fermentation	167:187	in vitro fermentation	167:187	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	2	9	theme	XG	528:529	arg1	rate					520:523	fermentation rate	507:523	fermentation rate of XG	507:529	Compared to individual polysaccharides, the fermentation extent of BC and fermentation rate of XG were promoted in BC&XG.					
33593569	2	9	theme	XG	528:529	arg1	extent					490:495	the fermentation extent	473:495	the fermentation extent of BC	473:501	Compared to individual polysaccharides, the fermentation extent of BC and fermentation rate of XG were promoted in BC&XG.					
33593569	1	10	theme	pellicle	254:261	arg1	BCXG					274:277	BCXG	274:277	BCXG	274:277	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	1	10	theme	pellicle	254:261	arg1	formation					263:271	pellicle formation	254:271	pellicle formation (BCXG)	254:278	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	4	11	theme	short	728:732	arg1	production					751:760	short chain fatty acid production	728:760	short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG	728:821	This combination explains the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG.					
33593569	5	12	theme	Microbial	824:832	arg1	dynamics					844:851	Microbial community dynamics	824:851	Microbial community dynamics for each substrate	824:870	Microbial community dynamics for each substrate were consistent with the corresponding polysaccharide degradation.					
33593569	4	13	theme	fatty	740:744	arg1	acid					746:749	chain fatty acid	734:749	short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG	728:821	This combination explains the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG.					
33593569	1	14	theme	same	404:407	arg1	BC					409:410	the same BC	400:410	the same BC	400:410	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	5	15	theme	community	834:842	arg1	dynamics					844:851	Microbial community dynamics	824:851	Microbial community dynamics for each substrate	824:870	Microbial community dynamics for each substrate were consistent with the corresponding polysaccharide degradation.					
33593569	0	16	theme	cellulose	15:23	arg1	Interaction					0:10	Interaction	0:10	Interaction of cellulose and xyloglucan	0:38	Interaction of cellulose and xyloglucan influences in vitro fermentation outcomes.					
33593569	3	17	from	composite	579:587	arg1	embedded					558:565	embedded	558:565	embedded	558:565	XG embedded in the BCXG composite was degraded less than in BC&XG, while more cellulose in BCXG was fermented than in BC&XG.					
33593569	3	18	theme	more	628:631	arg1	cellulose					633:641	more cellulose	628:641	more cellulose in BCXG	628:649	XG embedded in the BCXG composite was degraded less than in BC&XG, while more cellulose in BCXG was fermented than in BC&XG.					
33593569	2	19	theme	individual	445:454	arg1	polysaccharides					456:470	individual polysaccharides	445:470	individual polysaccharides	445:470	Compared to individual polysaccharides, the fermentation extent of BC and fermentation rate of XG were promoted in BC&XG.					
33593569	5	20	theme	polysaccharide	911:924	arg1	degradation					926:936	the corresponding polysaccharide degradation	893:936	the corresponding polysaccharide degradation	893:936	Microbial community dynamics for each substrate were consistent with the corresponding polysaccharide degradation.					
33593569	0	21	theme	xyloglucan	29:38	arg1	Interaction					0:10	Interaction	0:10	Interaction of cellulose and xyloglucan	0:38	Interaction of cellulose and xyloglucan influences in vitro fermentation outcomes.					
33593569	4	22	theme	chain	734:738	arg1	acid					746:749	chain fatty acid	734:749	short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG	728:821	This combination explains the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG.					
33593569	0	23	dep	in	51:52	arg1	vitro					54:58	vitro	54:58	vitro	54:58	Interaction of cellulose and xyloglucan influences in vitro fermentation outcomes.					
33593569	4	24	theme	similar	710:716	arg1	amount					718:723	the similar amount	706:723	the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG	706:821	This combination explains the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG.					
33593569	4	24	theme	similar	710:716	arg1	production					751:760	short chain fatty acid production	728:760	short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG	728:821	This combination explains the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG.					
33593569	1	25	theme	interactions	113:124	arg1	effects					102:108	the effects	98:108	the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation	98:187	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	0	26	theme	in	51:52	arg1	outcomes					73:80	in vitro fermentation outcomes	51:80	in vitro fermentation outcomes	51:80	Interaction of cellulose and xyloglucan influences in vitro fermentation outcomes.					
33593569	3	27	from	BC&XG	673:677	arg1	fermented					655:663	fermented	655:663	fermented	655:663	XG embedded in the BCXG composite was degraded less than in BC&XG, while more cellulose in BCXG was fermented than in BC&XG.					
33593569	1	28	dep	in	167:168	arg1	vitro					170:174	vitro	170:174	vitro	170:174	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	1	29	theme	XG	415:416	arg1	ratio					418:422	XG ratio	415:422	XG ratio of BCXG	415:430	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	1	30	from	effects	102:108	arg1	fermentation					176:187	in vitro fermentation	167:187	in vitro fermentation	167:187	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	2	31	theme	fermentation	477:488	arg1	extent					490:495	the fermentation extent	473:495	the fermentation extent of BC	473:501	Compared to individual polysaccharides, the fermentation extent of BC and fermentation rate of XG were promoted in BC&XG.					
33593569	3	32	theme	BCXG	574:577	arg1	composite					579:587	the BCXG composite	570:587	the BCXG composite	570:587	XG embedded in the BCXG composite was degraded less than in BC&XG, while more cellulose in BCXG was fermented than in BC&XG.					
33593569	6	33	theme	multiple	1030:1037	arg1	ways					1039:1042	multiple ways	1030:1042	multiple ways	1030:1042	Thus, interactions between cellulose and XG are shown to influence their fermentability in multiple ways.					
33593569	1	34	theme	bacterial	205:213	arg1	BC					226:227	BC	226:227	BC	226:227	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	1	34	theme	bacterial	205:213	arg1	cellulose					215:223	bacterial cellulose	205:223	bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG)	205:278	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	4	35	theme	fermentation	783:794	arg1	process					796:802	the fermentation process	779:802	the fermentation process	779:802	This combination explains the similar amount of short chain fatty acid production noted throughout the fermentation process for BCXG and BC&XG.					
33593569	3	36	theme	embedded	558:565	arg1	XG					555:556	XG	555:556	XG embedded in the BCXG composite	555:587	XG embedded in the BCXG composite was degraded less than in BC&XG, while more cellulose in BCXG was fermented than in BC&XG.					
33593569	1	37	theme	cellulose	215:223	arg1	composite					192:200	a composite	190:200	a composite	190:200	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	1	37	theme	cellulose	215:223	arg1	cellulose					215:223	bacterial cellulose	205:223	bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG)	205:278	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	3	38	from	embedded	558:565	arg1	composite					579:587	the BCXG composite	570:587	the BCXG composite	570:587	XG embedded in the BCXG composite was degraded less than in BC&XG, while more cellulose in BCXG was fermented than in BC&XG.					
33593569	3	39	from	cellulose	633:641	arg1	BCXG					646:649	BCXG	646:649	BCXG	646:649	XG embedded in the BCXG composite was degraded less than in BC&XG, while more cellulose in BCXG was fermented than in BC&XG.					
33593569	1	40	theme	BCXG	427:430	arg1	ratio					418:422	XG ratio	415:422	XG ratio of BCXG	415:430	To investigate the effects of interactions between cellulose and xyloglucan (XG) on in vitro fermentation, a composite of bacterial cellulose (BC) incorporating XG during pellicle formation (BCXG), was fermented using a human faecal inoculum, and compared with BC, XG and a mixture (BC&XG) physically blended to have the same BC to XG ratio of BCXG.					
33593569	0	41	theme	fermentation	60:71	arg1	outcomes					73:80	in vitro fermentation outcomes	51:80	in vitro fermentation outcomes	51:80	Interaction of cellulose and xyloglucan influences in vitro fermentation outcomes.					
33593569	5	42	theme	corresponding	897:909	arg1	degradation					926:936	the corresponding polysaccharide degradation	893:936	the corresponding polysaccharide degradation	893:936	Microbial community dynamics for each substrate were consistent with the corresponding polysaccharide degradation.					
33593569	6	43	from	fermentability	1012:1025	arg1	ways					1039:1042	multiple ways	1030:1042	multiple ways	1030:1042	Thus, interactions between cellulose and XG are shown to influence their fermentability in multiple ways.					
32059888	4	0	theme	different	883:891	arg1	degrees					893:899	different degrees	883:899	different degrees of esterification	883:917	The FTIR spectra indicated that these polysaccharides had different degrees of esterification.					
32059888	1	1	theme	HPGPC	285:289	arg1	distribution					357:368	molecular weight distribution	340:368	molecular weight distribution	340:368	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	1	theme	HPGPC	285:289	arg1	mapping					303:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	1	theme	HPGPC	285:289	arg1	composition					327:337	monosaccharide composition	312:337	monosaccharide composition	312:337	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	2	theme	odoratum	200:207	arg1	batches					117:123	14 batches	114:123	14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO)	114:212	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	3	theme	P.	156:157	arg1	PC					170:171	PC	170:171	PC	170:171	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	3	theme	P.	156:157	arg1	cyrtonema					159:167	P. cyrtonema	156:167	P. cyrtonema (PC)	156:172	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	4	dep	infrared	392:399	arg1	FTIR					402:405	FTIR	402:405	FTIR	402:405	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	0	5	from	Characterisation	0:15	arg1	spp					88:90	four common Polygonatum spp	64:90	four common Polygonatum spp	64:90	Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.					
32059888	6	6	theme	chemical	1096:1103	arg1	composition					1105:1115	chemical composition	1096:1115	chemical composition	1096:1115	and was helpful in understanding the varied functions of different Polygonatum spp., based on chemical composition.					
32059888	3	7	theme	composition	709:719	arg1	analysis					682:689	The analysis	678:689	The analysis of monosaccharide composition and HPGPC saccharide mapping	678:748	The analysis of monosaccharide composition and HPGPC saccharide mapping proved that P1 and P2 were composed of pectins and fructans, respectively.					
32059888	4	8	theme	FTIR	829:832	arg1	spectra					834:840	The FTIR spectra	825:840	The FTIR spectra	825:840	The FTIR spectra indicated that these polysaccharides had different degrees of esterification.					
32059888	5	9	theme	Polygonatum	985:995	arg1	spp					997:999	Polygonatum spp	985:999	Polygonatum spp	985:999	This study provided a systematic profiling of polysaccharides of Polygonatum spp.					
32059888	2	10	theme	main	662:665	arg1	P2					673:674	P2	673:674	P2	673:674	Results showed that polysaccharides from PS, PC and PK exhibited two different molecular weight fractions and that one was more than 4.1 × 105 Da (P1) and the other was 2.8-5.4 × 103 Da (P2); while the polysaccharides from PO displayed only one main peak (P2).					
32059888	2	10	theme	main	662:665	arg1	peak					667:670	only one main peak	653:670	only one main peak (P2)	653:675	Results showed that polysaccharides from PS, PC and PK exhibited two different molecular weight fractions and that one was more than 4.1 × 105 Da (P1) and the other was 2.8-5.4 × 103 Da (P2); while the polysaccharides from PO displayed only one main peak (P2).					
32059888	1	11	theme	cyrtonema	159:167	arg1	batches					117:123	14 batches	114:123	14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO)	114:212	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	4	12	theme	esterification	904:917	arg1	degrees					893:899	different degrees	883:899	different degrees of esterification	883:917	The FTIR spectra indicated that these polysaccharides had different degrees of esterification.					
32059888	1	13	theme	monosaccharide	312:325	arg1	mapping					303:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	13	theme	monosaccharide	312:325	arg1	composition					327:337	monosaccharide composition	312:337	monosaccharide composition	312:337	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	3	14	theme	HPGPC	725:729	arg1	mapping					742:748	HPGPC saccharide mapping	725:748	HPGPC saccharide mapping	725:748	The analysis of monosaccharide composition and HPGPC saccharide mapping proved that P1 and P2 were composed of pectins and fructans, respectively.					
32059888	6	15	theme	spp.	1081:1084	arg1	functions					1046:1054	the varied functions	1035:1054	the varied functions	1035:1054	and was helpful in understanding the varied functions of different Polygonatum spp., based on chemical composition.					
32059888	5	16	theme	polysaccharides	966:980	arg1	profiling					953:961	a systematic profiling	940:961	a systematic profiling of polysaccharides of Polygonatum spp	940:999	This study provided a systematic profiling of polysaccharides of Polygonatum spp.					
32059888	2	17	from	PK	469:470	arg1	polysaccharides					437:451	polysaccharides	437:451	polysaccharides from PS, PC and PK	437:470	Results showed that polysaccharides from PS, PC and PK exhibited two different molecular weight fractions and that one was more than 4.1 × 105 Da (P1) and the other was 2.8-5.4 × 103 Da (P2); while the polysaccharides from PO displayed only one main peak (P2).					
32059888	6	18	theme	Polygonatum	1069:1079	arg1	spp.					1081:1084	different Polygonatum spp.	1059:1084	different Polygonatum spp.	1059:1084	and was helpful in understanding the varied functions of different Polygonatum spp., based on chemical composition.					
32059888	1	19	theme	molecular	340:348	arg1	distribution					357:368	molecular weight distribution	340:368	molecular weight distribution	340:368	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	19	theme	molecular	340:348	arg1	mapping					303:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	2	20	theme	weight	506:511	arg1	fractions					513:521	two different molecular weight fractions	482:521	two different molecular weight fractions	482:521	Results showed that polysaccharides from PS, PC and PK exhibited two different molecular weight fractions and that one was more than 4.1 × 105 Da (P1) and the other was 2.8-5.4 × 103 Da (P2); while the polysaccharides from PO displayed only one main peak (P2).					
32059888	6	21	theme	different	1059:1067	arg1	spp.					1081:1084	different Polygonatum spp.	1059:1084	different Polygonatum spp.	1059:1084	and was helpful in understanding the varied functions of different Polygonatum spp., based on chemical composition.					
32059888	0	22	theme	saccharide	21:30	arg1	mapping					32:38	saccharide mapping	21:38	saccharide mapping	21:38	Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.					
32059888	2	23	theme	molecular	496:504	arg1	fractions					513:521	two different molecular weight fractions	482:521	two different molecular weight fractions	482:521	Results showed that polysaccharides from PS, PC and PK exhibited two different molecular weight fractions and that one was more than 4.1 × 105 Da (P1) and the other was 2.8-5.4 × 103 Da (P2); while the polysaccharides from PO displayed only one main peak (P2).					
32059888	1	24	theme	P.	175:176	arg1	batches					117:123	14 batches	114:123	14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO)	114:212	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	0	25	theme	polysaccharides	43:57	arg1	Characterisation					0:15	Characterisation	0:15	Characterisation	0:15	Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.					
32059888	0	25	theme	polysaccharides	43:57	arg1	mapping					32:38	saccharide mapping	21:38	saccharide mapping	21:38	Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.					
32059888	6	26	theme	varied	1039:1044	arg1	functions					1046:1054	the varied functions	1035:1054	the varied functions	1035:1054	and was helpful in understanding the varied functions of different Polygonatum spp., based on chemical composition.					
32059888	2	27	theme	different	486:494	arg1	fractions					513:521	two different molecular weight fractions	482:521	two different molecular weight fractions	482:521	Results showed that polysaccharides from PS, PC and PK exhibited two different molecular weight fractions and that one was more than 4.1 × 105 Da (P1) and the other was 2.8-5.4 × 103 Da (P2); while the polysaccharides from PO displayed only one main peak (P2).					
32059888	0	28	from	spp	88:90	arg1	polysaccharides					43:57	polysaccharides	43:57	polysaccharides from four common Polygonatum spp	43:90	Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.					
32059888	0	28	from	spp	88:90	arg1	Characterisation					0:15	Characterisation	0:15	Characterisation	0:15	Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.					
32059888	0	28	from	spp	88:90	arg1	mapping					32:38	saccharide mapping	21:38	saccharide mapping	21:38	Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.					
32059888	1	29	theme	high-performance	237:252	arg1	distribution					357:368	molecular weight distribution	340:368	molecular weight distribution	340:368	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	29	theme	high-performance	237:252	arg1	mapping					303:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	29	theme	high-performance	237:252	arg1	composition					327:337	monosaccharide composition	312:337	monosaccharide composition	312:337	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	3	30	theme	saccharide	731:740	arg1	mapping					742:748	HPGPC saccharide mapping	725:748	HPGPC saccharide mapping	725:748	The analysis of monosaccharide composition and HPGPC saccharide mapping proved that P1 and P2 were composed of pectins and fructans, respectively.					
32059888	1	31	theme	weight	350:355	arg1	distribution					357:368	molecular weight distribution	340:368	molecular weight distribution	340:368	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	31	theme	weight	350:355	arg1	mapping					303:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	2	32	from	PO	640:641	arg1	polysaccharides					619:633	the polysaccharides	615:633	the polysaccharides from PO	615:641	Results showed that polysaccharides from PS, PC and PK exhibited two different molecular weight fractions and that one was more than 4.1 × 105 Da (P1) and the other was 2.8-5.4 × 103 Da (P2); while the polysaccharides from PO displayed only one main peak (P2).					
32059888	1	33	theme	gel	254:256	arg1	distribution					357:368	molecular weight distribution	340:368	molecular weight distribution	340:368	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	33	theme	gel	254:256	arg1	mapping					303:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	33	theme	gel	254:256	arg1	composition					327:337	monosaccharide composition	312:337	monosaccharide composition	312:337	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	2	34	from	PS	458:459	arg1	polysaccharides					437:451	polysaccharides	437:451	polysaccharides from PS, PC and PK	437:470	Results showed that polysaccharides from PS, PC and PK exhibited two different molecular weight fractions and that one was more than 4.1 × 105 Da (P1) and the other was 2.8-5.4 × 103 Da (P2); while the polysaccharides from PO displayed only one main peak (P2).					
32059888	1	35	theme	saccharide	292:301	arg1	distribution					357:368	molecular weight distribution	340:368	molecular weight distribution	340:368	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	35	theme	saccharide	292:301	arg1	mapping					303:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	35	theme	saccharide	292:301	arg1	composition					327:337	monosaccharide composition	312:337	monosaccharide composition	312:337	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	36	theme	permeation	258:267	arg1	distribution					357:368	molecular weight distribution	340:368	molecular weight distribution	340:368	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	36	theme	permeation	258:267	arg1	mapping					303:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	36	theme	permeation	258:267	arg1	composition					327:337	monosaccharide composition	312:337	monosaccharide composition	312:337	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	5	37	theme	systematic	942:951	arg1	profiling					953:961	a systematic profiling	940:961	a systematic profiling of polysaccharides of Polygonatum spp	940:999	This study provided a systematic profiling of polysaccharides of Polygonatum spp.					
32059888	5	38	theme	spp	997:999	arg1	polysaccharides					966:980	polysaccharides	966:980	polysaccharides of Polygonatum spp	966:999	This study provided a systematic profiling of polysaccharides of Polygonatum spp.					
32059888	1	39	theme	chromatography	269:282	arg1	distribution					357:368	molecular weight distribution	340:368	molecular weight distribution	340:368	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	39	theme	chromatography	269:282	arg1	mapping					303:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	high-performance gel permeation chromatography (HPGPC) saccharide mapping	237:309	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	39	theme	chromatography	269:282	arg1	composition					327:337	monosaccharide composition	312:337	monosaccharide composition	312:337	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	2	40	from	PC	462:463	arg1	polysaccharides					437:451	polysaccharides	437:451	polysaccharides from PS, PC and PK	437:470	Results showed that polysaccharides from PS, PC and PK exhibited two different molecular weight fractions and that one was more than 4.1 × 105 Da (P1) and the other was 2.8-5.4 × 103 Da (P2); while the polysaccharides from PO displayed only one main peak (P2).					
32059888	1	41	theme	sibiricum	140:148	arg1	batches					117:123	14 batches	114:123	14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO)	114:212	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	1	42	from	batches	117:123	arg1	Polysaccharides					93:107	Polysaccharides	93:107	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO)	93:212	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	4	43	contain	had	879:881	arg1	polysaccharides					863:877	these polysaccharides	857:877	these polysaccharides	857:877	The FTIR spectra indicated that these polysaccharides had different degrees of esterification.					
32059888	4	43	contain	had	879:881	arg2	degrees					893:899	different degrees	883:899	different degrees of esterification	883:917	The FTIR spectra indicated that these polysaccharides had different degrees of esterification.					
32059888	3	44	theme	mapping	742:748	arg1	analysis					682:689	The analysis	678:689	The analysis of monosaccharide composition and HPGPC saccharide mapping	678:748	The analysis of monosaccharide composition and HPGPC saccharide mapping proved that P1 and P2 were composed of pectins and fructans, respectively.					
32059888	3	45	theme	monosaccharide	694:707	arg1	composition					709:719	monosaccharide composition	694:719	monosaccharide composition	694:719	The analysis of monosaccharide composition and HPGPC saccharide mapping proved that P1 and P2 were composed of pectins and fructans, respectively.					
32059888	0	46	theme	Polygonatum	76:86	arg1	spp					88:90	four common Polygonatum spp	64:90	four common Polygonatum spp	64:90	Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.					
32059888	1	47	theme	infrared	392:399	arg1	spectra					408:414	infrared (FTIR) spectra	392:414	infrared (FTIR) spectra	392:414	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32059888	0	48	theme	common	69:74	arg1	spp					88:90	four common Polygonatum spp	64:90	four common Polygonatum spp	64:90	Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.					
32059888	0	49	from	mapping	32:38	arg1	spp					88:90	four common Polygonatum spp	64:90	four common Polygonatum spp	64:90	Characterisation and saccharide mapping of polysaccharides from four common Polygonatum spp.					
32059888	1	50	dep	P.	175:176	arg1	kingianum					178:186	P. kingianum (PK)	175:191	P. kingianum (PK)	175:191	Polysaccharides from 14 batches of Polygonatum sibiricum (PS), P. cyrtonema (PC), P. kingianum (PK) and P. odoratum (PO) were compared based on high-performance gel permeation chromatography (HPGPC) saccharide mapping, monosaccharide composition, molecular weight distribution and Fourier transform infrared (FTIR) spectra.					
32342633	4	0	theme	strategy	603:610	arg1	features					577:584	Key features	573:584	Key features of the synthetic strategy	573:610	Key features of the synthetic strategy include the use of di-tert-butylsilylidene directed α-galactosylation methodology and regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt).					
32342633	5	1	theme	hydrogen	1037:1044	arg1	one					994:996	one	994:996	one	994:996	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	1	theme	hydrogen	1037:1044	arg1	bond					1046:1049	a non-conventional C-H⋅⋅⋅O hydrogen bond	1010:1049	a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i)	1010:1107	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	1	theme	hydrogen	1037:1044	arg1	structure					946:954	an elongated, almost straight, structure	915:954	an elongated, almost straight, structure	915:954	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	2	theme	elongated	918:926	arg1	structure					946:954	an elongated, almost straight, structure	915:954	an elongated, almost straight, structure	915:954	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	3	theme	GalN	813:816	arg1	oligomers					829:837	the Gal, GalN and GalNAc oligomers	804:837	the Gal, GalN and GalNAc oligomers	804:837	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	1	4	theme	prominent	193:201	arg1	component					213:221	a prominent cell wall component	191:221	a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus	191:280	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	1	4	theme	prominent	193:201	arg1	Galactosaminogalactan					160:180	Galactosaminogalactan	160:180	Galactosaminogalactan (GAG)	160:186	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	0	5	theme	α-N-Acetyl	125:134	arg1	Linkages					150:157	α-N-Acetyl Galactosamine Linkages	125:157	α-N-Acetyl Galactosamine Linkages	125:157	Synthesis and Structural Analysis of Aspergillus fumigatus Galactosaminogalactans Featuring α-Galactose, α-Galactosamine and α-N-Acetyl Galactosamine Linkages.					
32342633	5	6	theme	GalNAc	822:827	arg1	oligomers					829:837	the Gal, GalN and GalNAc oligomers	804:837	the Gal, GalN and GalNAc oligomers	804:837	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	6	7	theme	chain	1204:1208	arg1	axis					1210:1213	the oligosaccharide main chain axis	1179:1213	the oligosaccharide main chain axis	1179:1213	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	3	8	contain	containing	493:502	arg1	specimens					483:491	specimens	483:491	specimens containing α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	483:570	To enable biochemical studies, a library of GAG-fragments was constructed featuring specimens containing α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages.					
32342633	3	8	contain	containing	493:502	arg2	linkages					563:570	α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	504:570	α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	504:570	To enable biochemical studies, a library of GAG-fragments was constructed featuring specimens containing α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages.					
32342633	2	9	theme	α-1,4-linked	325:336	arg1	galactose					338:346	α-1,4-linked galactose	325:346	α-1,4-linked galactose	325:346	GAG is a heteropolysaccharide composed of α-1,4-linked galactose, galactosamine and N-acetylgalactosamine residues.					
32342633	1	10	theme	cell	203:206	arg1	component					213:221	a prominent cell wall component	191:221	a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus	191:280	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	1	10	theme	cell	203:206	arg1	Galactosaminogalactan					160:180	Galactosaminogalactan	160:180	Galactosaminogalactan (GAG)	160:186	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	0	11	theme	Galactosamine	136:148	arg1	Linkages					150:157	α-N-Acetyl Galactosamine Linkages	125:157	α-N-Acetyl Galactosamine Linkages	125:157	Synthesis and Structural Analysis of Aspergillus fumigatus Galactosaminogalactans Featuring α-Galactose, α-Galactosamine and α-N-Acetyl Galactosamine Linkages.					
32342633	4	12	theme	synthetic	593:601	arg1	strategy					603:610	the synthetic strategy	589:610	the synthetic strategy	589:610	Key features of the synthetic strategy include the use of di-tert-butylsilylidene directed α-galactosylation methodology and regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt).					
32342633	6	13	theme	main	1199:1202	arg1	chain					1204:1208	the oligosaccharide main chain	1179:1208	the oligosaccharide main chain axis	1179:1213	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	1	14	theme	wall	208:211	arg1	component					213:221	a prominent cell wall component	191:221	a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus	191:280	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	1	14	theme	wall	208:211	arg1	Galactosaminogalactan					160:180	Galactosaminogalactan	160:180	Galactosaminogalactan (GAG)	160:186	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	5	15	theme	structure	946:954	arg1	bond					1046:1049	a non-conventional C-H⋅⋅⋅O hydrogen bond	1010:1049	a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i)	1010:1107	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	15	theme	structure	946:954	arg1	one					994:996	one	994:996	one	994:996	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	15	theme	structure	946:954	arg1	structure					946:954	an elongated, almost straight, structure	915:954	an elongated, almost straight, structure	915:954	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	6	16	theme	oligosaccharide	1183:1197	arg1	chain					1204:1208	the oligosaccharide main chain	1179:1208	the oligosaccharide main chain axis	1179:1213	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	5	17	theme	residue	1069:1075	arg1	H5					1059:1060	H5	1059:1060	H5 of the residue (i+1)	1059:1081	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	17	theme	residue	1069:1075	arg1	O3					1087:1088	O3	1087:1088	O3 of the residue (i)	1087:1107	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	2	18	link	α-1,4-linked	325:336	arg1	galactose					338:346	α-1,4-linked galactose	325:346	α-1,4-linked galactose	325:346	GAG is a heteropolysaccharide composed of α-1,4-linked galactose, galactosamine and N-acetylgalactosamine residues.					
32342633	5	19	theme	Gal	808:810	arg1	oligomers					829:837	the Gal, GalN and GalNAc oligomers	804:837	the Gal, GalN and GalNAc oligomers	804:837	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	6	20	theme	available	1249:1257	arg1	substituents					1142:1153	the C-2 substituents	1134:1153	the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners	1134:1316	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	3	21	theme	α-galactosamine	518:532	arg1	linkages					563:570	α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	504:570	α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	504:570	To enable biochemical studies, a library of GAG-fragments was constructed featuring specimens containing α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages.					
32342633	5	22	dep	elongated	918:926	arg1	straight					936:943	straight	936:943	straight	936:943	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	4	23	theme	methodology	682:692	arg1	reactions					726:734	regioselective benzoylation reactions	698:734	regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt)	698:778	Key features of the synthetic strategy include the use of di-tert-butylsilylidene directed α-galactosylation methodology and regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt).					
32342633	4	23	theme	methodology	682:692	arg1	use					624:626	the use	620:626	the use of di-tert-butylsilylidene directed α-galactosylation methodology	620:692	Key features of the synthetic strategy include the use of di-tert-butylsilylidene directed α-galactosylation methodology and regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt).					
32342633	5	24	theme	Structural	781:790	arg1	analysis					792:799	Structural analysis	781:799	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches	781:879	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	6	25	theme	C-2	1138:1140	arg1	substituents					1142:1153	the C-2 substituents	1134:1153	the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners	1134:1316	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	5	26	theme	inter-residue	971:983	arg1	H-bonds					985:991	inter-residue H-bonds	971:991	inter-residue H-bonds	971:991	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	3	27	theme	GAG-fragments	443:455	arg1	library					432:438	a library	430:438	a library of GAG-fragments	430:455	To enable biochemical studies, a library of GAG-fragments was constructed featuring specimens containing α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages.					
32342633	4	28	theme	benzoylation	713:724	arg1	reactions					726:734	regioselective benzoylation reactions	698:734	regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt)	698:778	Key features of the synthetic strategy include the use of di-tert-butylsilylidene directed α-galactosylation methodology and regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt).					
32342633	5	29	theme	NMR	859:861	arg1	approaches					870:879	NMR and MD approaches	859:879	approaches	870:879	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	6	30	theme	other	1295:1299	arg1	partners					1309:1316	other binding partners	1295:1316	other binding partners	1295:1316	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	2	31	dep	galactose	338:346	arg1	residues					389:396	residues	389:396	residues	389:396	GAG is a heteropolysaccharide composed of α-1,4-linked galactose, galactosamine and N-acetylgalactosamine residues.					
32342633	1	32	theme	opportunistic	230:242	arg1	fumigatus					272:280	the opportunistic fungal pathogen Aspergillus fumigatus	226:280	the opportunistic fungal pathogen Aspergillus fumigatus	226:280	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	0	33	theme	Aspergillus	37:47	arg1	Galactosaminogalactans					59:80	Aspergillus fumigatus Galactosaminogalactans	37:80	Aspergillus fumigatus Galactosaminogalactans Featuring α-Galactose, α-Galactosamine and α-N-Acetyl Galactosamine Linkages	37:157	Synthesis and Structural Analysis of Aspergillus fumigatus Galactosaminogalactans Featuring α-Galactose, α-Galactosamine and α-N-Acetyl Galactosamine Linkages.					
32342633	4	34	theme	regioselective	698:711	arg1	reactions					726:734	regioselective benzoylation reactions	698:734	regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt)	698:778	Key features of the synthetic strategy include the use of di-tert-butylsilylidene directed α-galactosylation methodology and regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt).					
32342633	1	35	theme	fungal	244:249	arg1	fumigatus					272:280	the opportunistic fungal pathogen Aspergillus fumigatus	226:280	the opportunistic fungal pathogen Aspergillus fumigatus	226:280	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	6	36	theme	perpendicular	1162:1174	arg1	substituents					1142:1153	the C-2 substituents	1134:1153	the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners	1134:1316	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	3	37	theme	galactosamine	549:561	arg1	linkages					563:570	α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	504:570	α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	504:570	To enable biochemical studies, a library of GAG-fragments was constructed featuring specimens containing α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages.					
32342633	3	38	theme	α-galactose-	504:515	arg1	linkages					563:570	α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	504:570	α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	504:570	To enable biochemical studies, a library of GAG-fragments was constructed featuring specimens containing α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages.					
32342633	4	39	theme	di-tert-butylsilylidene	631:653	arg1	methodology					682:692	di-tert-butylsilylidene directed α-galactosylation methodology	631:692	di-tert-butylsilylidene directed α-galactosylation methodology	631:692	Key features of the synthetic strategy include the use of di-tert-butylsilylidene directed α-galactosylation methodology and regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt).					
32342633	5	40	theme	MD	867:868	arg1	approaches					870:879	NMR and MD approaches	859:879	approaches	870:879	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	4	41	theme	Key	573:575	arg1	features					577:584	Key features	573:584	Key features of the synthetic strategy	573:610	Key features of the synthetic strategy include the use of di-tert-butylsilylidene directed α-galactosylation methodology and regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt).					
32342633	1	42	theme	pathogen	251:258	arg1	fumigatus					272:280	the opportunistic fungal pathogen Aspergillus fumigatus	226:280	the opportunistic fungal pathogen Aspergillus fumigatus	226:280	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	0	43	theme	Galactosaminogalactans	59:80	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and Structural Analysis of Aspergillus fumigatus Galactosaminogalactans Featuring α-Galactose, α-Galactosamine and α-N-Acetyl Galactosamine Linkages.					
32342633	0	43	theme	Galactosaminogalactans	59:80	arg1	Analysis					25:32	Structural Analysis	14:32	Structural Analysis of Aspergillus fumigatus Galactosaminogalactans Featuring α-Galactose, α-Galactosamine and α-N-Acetyl Galactosamine Linkages	14:157	Synthesis and Structural Analysis of Aspergillus fumigatus Galactosaminogalactans Featuring α-Galactose, α-Galactosamine and α-N-Acetyl Galactosamine Linkages.					
32342633	5	44	theme	approaches	870:879	arg1	combination					844:854	a combination	842:854	a combination of NMR and MD approaches	842:879	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	45	theme	oligomers	829:837	arg1	analysis					792:799	Structural analysis	781:799	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches	781:879	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	6	46	with	interactions	1263:1274	arg1	partners					1309:1316	other binding partners	1295:1316	other binding partners	1295:1316	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	6	46	with	interactions	1263:1274	arg1	antibodies					1281:1290	antibodies	1281:1290	antibodies	1281:1290	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	0	47	dep	Aspergillus	37:47	arg1	fumigatus					49:57	fumigatus	49:57	fumigatus	49:57	Synthesis and Structural Analysis of Aspergillus fumigatus Galactosaminogalactans Featuring α-Galactose, α-Galactosamine and α-N-Acetyl Galactosamine Linkages.					
32342633	6	48	theme	bulk	1232:1235	arg1	solvent					1237:1243	the bulk solvent	1228:1243	the bulk solvent	1228:1243	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	4	49	theme	α-galactosylation	664:680	arg1	methodology					682:692	di-tert-butylsilylidene directed α-galactosylation methodology	631:692	di-tert-butylsilylidene directed α-galactosylation methodology	631:692	Key features of the synthetic strategy include the use of di-tert-butylsilylidene directed α-galactosylation methodology and regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt).					
32342633	4	50	theme	directed	655:662	arg1	methodology					682:692	di-tert-butylsilylidene directed α-galactosylation methodology	631:692	di-tert-butylsilylidene directed α-galactosylation methodology	631:692	Key features of the synthetic strategy include the use of di-tert-butylsilylidene directed α-galactosylation methodology and regioselective benzoylation reactions using benzoyl-hydroxybenzotriazole (Bz-OBt).					
32342633	5	51	theme	residue	1097:1103	arg1	H5					1059:1060	H5	1059:1060	H5 of the residue (i+1)	1059:1081	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	51	theme	residue	1097:1103	arg1	O3					1087:1088	O3	1087:1088	O3 of the residue (i)	1087:1107	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	1	52	theme	Aspergillus	260:270	arg1	fumigatus					272:280	the opportunistic fungal pathogen Aspergillus fumigatus	226:280	the opportunistic fungal pathogen Aspergillus fumigatus	226:280	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	1	53	theme	fumigatus	272:280	arg1	component					213:221	a prominent cell wall component	191:221	a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus	191:280	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	1	53	theme	fumigatus	272:280	arg1	Galactosaminogalactan					160:180	Galactosaminogalactan	160:180	Galactosaminogalactan (GAG)	160:186	Galactosaminogalactan (GAG) is a prominent cell wall component of the opportunistic fungal pathogen Aspergillus fumigatus.					
32342633	6	54	theme	binding	1301:1307	arg1	partners					1309:1316	other binding partners	1295:1316	other binding partners	1295:1316	The structures position the C-2 substituents almost perpendicular to the oligosaccharide main chain axis, pointing to the bulk solvent and available for interactions with antibodies or other binding partners.					
32342633	5	55	theme	non-conventional	1012:1027	arg1	one					994:996	one	994:996	one	994:996	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	55	theme	non-conventional	1012:1027	arg1	bond					1046:1049	a non-conventional C-H⋅⋅⋅O hydrogen bond	1010:1049	a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i)	1010:1107	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	55	theme	non-conventional	1012:1027	arg1	structure					946:954	an elongated, almost straight, structure	915:954	an elongated, almost straight, structure	915:954	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	3	56	theme	α-N-acetyl	538:547	arg1	linkages					563:570	α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	504:570	α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages	504:570	To enable biochemical studies, a library of GAG-fragments was constructed featuring specimens containing α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages.					
32342633	3	57	theme	biochemical	409:419	arg1	studies					421:427	biochemical studies	409:427	biochemical studies	409:427	To enable biochemical studies, a library of GAG-fragments was constructed featuring specimens containing α-galactose-, α-galactosamine and α-N-acetyl galactosamine linkages.					
32342633	0	58	theme	Structural	14:23	arg1	Analysis					25:32	Structural Analysis	14:32	Structural Analysis of Aspergillus fumigatus Galactosaminogalactans Featuring α-Galactose, α-Galactosamine and α-N-Acetyl Galactosamine Linkages	14:157	Synthesis and Structural Analysis of Aspergillus fumigatus Galactosaminogalactans Featuring α-Galactose, α-Galactosamine and α-N-Acetyl Galactosamine Linkages.					
32342633	5	59	theme	C-H⋅⋅⋅O	1029:1035	arg1	one					994:996	one	994:996	one	994:996	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	59	theme	C-H⋅⋅⋅O	1029:1035	arg1	bond					1046:1049	a non-conventional C-H⋅⋅⋅O hydrogen bond	1010:1049	a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i)	1010:1107	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32342633	5	59	theme	C-H⋅⋅⋅O	1029:1035	arg1	structure					946:954	an elongated, almost straight, structure	915:954	an elongated, almost straight, structure	915:954	Structural analysis of the Gal, GalN and GalNAc oligomers by a combination of NMR and MD approaches revealed that the oligomers adopt an elongated, almost straight, structure, stabilized by inter-residue H-bonds, one of which is a non-conventional C-H⋅⋅⋅O hydrogen bond between H5 of the residue (i+1) and O3 of the residue (i).					
32799166	0	0	theme	polysaccharides	83:97	arg1	Production					0:9	Production	0:9	Production	0:9	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	0	0	theme	polysaccharides	83:97	arg1	characteristics					28:42	physicochemical characteristics	12:42	physicochemical characteristics	12:42	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	0	0	theme	polysaccharides	83:97	arg1	activities					69:78	in vitro biological activities	49:78	in vitro biological activities of polysaccharides	49:97	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	1	1	theme	temperature	310:320	arg1	extraction					410:419	ultrasonic-assisted extraction	390:419	ultrasonic-assisted extraction (UAE)	390:425	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	1	1	theme	temperature	310:320	arg1	techniques					333:342	room temperature extraction techniques	305:342	room temperature extraction techniques	305:342	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	1	1	theme	temperature	310:320	arg1	partitioning					367:378	three-phase partitioning	355:378	three-phase partitioning (TPP)	355:384	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	0	2	theme	extraction	178:187	arg1	techniques					189:198	room temperature extraction techniques	161:198	room temperature extraction techniques	161:198	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	4	3	theme	lowest	1129:1134	arg1	Mws					1136:1138	the lowest Mws	1125:1138	the lowest Mws	1125:1138	Moreover, BPS-A, which had the lowest Mws, showed the best bile acid-binding capacity among the four BPSs.					
32799166	3	4	from	UAE	1010:1012	arg1	NaBH4					1039:1043	1.25 mol/L NaOH/0.05% NaBH4	1017:1043	1.25 mol/L NaOH/0.05% NaBH4	1017:1043	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	5	5	theme	great	1220:1224	arg1	potentials					1226:1235	great potentials	1220:1235	great potentials for the preparation of bioactive polysaccharides from fresh vegetables	1220:1306	This study had great potentials for the preparation of bioactive polysaccharides from fresh vegetables.					
32799166	1	6	dep	Momordica	278:286	arg1	L.					298:299	Momordica charantia L.	278:299	Momordica charantia L.	278:299	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	1	7	theme	extraction	322:331	arg1	extraction					410:419	ultrasonic-assisted extraction	390:419	ultrasonic-assisted extraction (UAE)	390:425	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	1	7	theme	extraction	322:331	arg1	techniques					333:342	room temperature extraction techniques	305:342	room temperature extraction techniques	305:342	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	1	7	theme	extraction	322:331	arg1	partitioning					367:378	three-phase partitioning	355:378	three-phase partitioning (TPP)	355:384	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	0	8	theme	temperature	166:176	arg1	techniques					189:198	room temperature extraction techniques	161:198	room temperature extraction techniques	161:198	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	2	9	theme	structural	658:667	arg1	characterization					669:684	preliminary structural characterization	646:684	preliminary structural characterization	646:684	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	3	10	from	residue	766:772	arg1	water					795:799	distilled water	785:799	distilled water	785:799	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	4	11	theme	bile	1157:1160	arg1	capacity					1175:1182	the best bile acid-binding capacity	1148:1182	the best bile acid-binding capacity among the four BPSs	1148:1202	Moreover, BPS-A, which had the lowest Mws, showed the best bile acid-binding capacity among the four BPSs.					
32799166	2	12	theme	molecular	595:603	arg1	weight					605:610	weight-average molecular weight	580:610	weight-average molecular weight (Mw)	580:615	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	12	theme	molecular	595:603	arg1	Mw					613:614	Mw	613:614	Mw	613:614	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	13	theme	preliminary	646:656	arg1	characterization					669:684	preliminary structural characterization	646:684	preliminary structural characterization	646:684	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	3	14	theme	stronger	857:864	arg1	capacities					878:887	stronger antioxidant capacities	857:887	stronger antioxidant capacities	857:887	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	15	theme	uronic	814:819	arg1	content					826:832	a higher uronic acid content	805:832	a higher uronic acid content (24.22%)	805:841	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	15	theme	uronic	814:819	arg1	%					840:840	24.22%	835:840	24.22%	835:840	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	2	16	theme	significant	511:521	arg1	influence					523:531	significant influence	511:531	significant influence	511:531	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	17	theme	weight-average	580:593	arg1	weight					605:610	weight-average molecular weight	580:610	weight-average molecular weight (Mw)	580:615	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	17	theme	weight-average	580:593	arg1	Mw					613:614	Mw	613:614	Mw	613:614	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	5	18	theme	polysaccharides	1270:1284	arg1	preparation					1245:1255	the preparation	1241:1255	the preparation of bioactive polysaccharides from fresh vegetables	1241:1306	This study had great potentials for the preparation of bioactive polysaccharides from fresh vegetables.					
32799166	5	19	theme	fresh	1291:1295	arg1	vegetables					1297:1306	fresh vegetables	1291:1306	fresh vegetables	1291:1306	This study had great potentials for the preparation of bioactive polysaccharides from fresh vegetables.					
32799166	1	20	theme	fresh	258:262	arg1	gourd					271:275	fresh bitter gourd	258:275	fresh bitter gourd	258:275	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	0	21	theme	bitter	119:124	arg1	gourd					126:130	fresh bitter gourd	113:130	fresh bitter gourd	113:130	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	2	22	theme	monosaccharide	618:631	arg1	composition					633:643	monosaccharide composition	618:643	monosaccharide composition	618:643	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	1	23	theme	different	440:448	arg1	solvents					450:457	different solvents	440:457	different solvents	440:457	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	3	24	theme	α-glycosidase	907:919	arg1	activities					932:941	α-amylase and α-glycosidase inhibitory activities	893:941	α-amylase and α-glycosidase inhibitory activities	893:941	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	0	25	theme	fresh	113:117	arg1	gourd					126:130	fresh bitter gourd	113:130	fresh bitter gourd	113:130	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	1	26	theme	three-phase	355:365	arg1	TPP					381:383	TPP	381:383	TPP	381:383	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	1	26	theme	three-phase	355:365	arg1	partitioning					367:378	three-phase partitioning	355:378	three-phase partitioning (TPP)	355:384	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	3	27	theme	%	1037:1037	arg1	NaBH4					1039:1043	1.25 mol/L NaOH/0.05% NaBH4	1017:1043	1.25 mol/L NaOH/0.05% NaBH4	1017:1043	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	0	28	dep	obtained	99:106	arg1	Momordica					133:141	Momordica	133:141	Momordica	133:141	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	3	29	theme	gourd	760:764	arg1	residue					766:772	the bitter gourd residue	749:772	the bitter gourd residue via UAE in distilled water	749:799	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	30	theme	α-amylase	893:901	arg1	activities					932:941	α-amylase and α-glycosidase inhibitory activities	893:941	α-amylase and α-glycosidase inhibitory activities	893:941	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	1	31	theme	bitter	264:269	arg1	gourd					271:275	fresh bitter gourd	258:275	fresh bitter gourd	258:275	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	3	32	theme	bitter	1071:1076	arg1	juice					1084:1088	bitter gourd juice	1071:1088	bitter gourd juice by TPP	1071:1095	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	33	theme	1.25 mol/L	1017:1026	arg1	NaBH4					1039:1043	1.25 mol/L NaOH/0.05% NaBH4	1017:1043	1.25 mol/L NaOH/0.05% NaBH4	1017:1043	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	34	theme	gourd	1078:1082	arg1	juice					1084:1088	bitter gourd juice	1071:1088	bitter gourd juice by TPP	1071:1095	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	35	contain	possessed	847:855	arg2	capacities					878:887	stronger antioxidant capacities	857:887	stronger antioxidant capacities	857:887	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	35	contain	possessed	847:855	arg1	sample					728:733	The BPS-W sample	718:733	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water	718:799	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	35	contain	possessed	847:855	arg2	activities					932:941	α-amylase and α-glycosidase inhibitory activities	893:941	α-amylase and α-glycosidase inhibitory activities	893:941	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	36	theme	NaOH/0.05	1028:1036	arg1	NaBH4					1039:1043	1.25 mol/L NaOH/0.05% NaBH4	1017:1043	1.25 mol/L NaOH/0.05% NaBH4	1017:1043	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	37	theme	acid	821:824	arg1	content					826:832	a higher uronic acid content	805:832	a higher uronic acid content (24.22%)	805:841	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	37	theme	acid	821:824	arg1	%					840:840	24.22%	835:840	24.22%	835:840	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	5	38	theme	bioactive	1260:1268	arg1	polysaccharides					1270:1284	bioactive polysaccharides	1260:1284	bioactive polysaccharides from fresh vegetables	1260:1306	This study had great potentials for the preparation of bioactive polysaccharides from fresh vegetables.					
32799166	0	39	theme	physicochemical	12:26	arg1	characteristics					28:42	physicochemical characteristics	12:42	physicochemical characteristics	12:42	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	2	40	theme	extraction	540:549	arg1	yield					551:555	extraction yield	540:555	extraction yield	540:555	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	3	41	theme	inhibitory	921:930	arg1	activities					932:941	α-amylase and α-glycosidase inhibitory activities	893:941	α-amylase and α-glycosidase inhibitory activities	893:941	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	5	42	from	preparation	1245:1255	arg1	vegetables					1297:1306	fresh vegetables	1291:1306	fresh vegetables	1291:1306	This study had great potentials for the preparation of bioactive polysaccharides from fresh vegetables.					
32799166	3	43	theme	bitter	753:758	arg1	residue					766:772	the bitter gourd residue	749:772	the bitter gourd residue via UAE in distilled water	749:799	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	44	from	UAE	969:971	arg1	BPS-J					1050:1054	BPS-J	1050:1054	BPS-J extracted from bitter gourd juice by TPP	1050:1095	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	44	from	UAE	969:971	arg1	acid					983:986	citric acid	976:986	citric acid	976:986	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	44	from	UAE	969:971	arg1	BPS-A					989:993	BPS-A	989:993	BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4	989:1043	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	0	45	dep	in	49:50	arg1	vitro					52:56	vitro	52:56	vitro	52:56	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	3	46	theme	distilled	785:793	arg1	water					795:799	distilled water	785:799	distilled water	785:799	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	2	47	theme	chemical	558:565	arg1	composition					567:577	chemical composition	558:577	chemical composition	558:577	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	0	48	theme	in	49:50	arg1	activities					69:78	in vitro biological activities	49:78	in vitro biological activities of polysaccharides	49:97	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	1	49	theme	ultrasonic-assisted	390:408	arg1	extraction					410:419	ultrasonic-assisted extraction	390:419	ultrasonic-assisted extraction (UAE)	390:425	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	1	49	theme	ultrasonic-assisted	390:408	arg1	UAE					422:424	UAE	422:424	UAE	422:424	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	2	50	theme	BPSs	712:715	arg1	weight					605:610	weight-average molecular weight	580:610	weight-average molecular weight (Mw)	580:615	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	50	theme	BPSs	712:715	arg1	composition					633:643	monosaccharide composition	618:643	monosaccharide composition	618:643	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	50	theme	BPSs	712:715	arg1	composition					567:577	chemical composition	558:577	chemical composition	558:577	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	50	theme	BPSs	712:715	arg1	Mw					613:614	Mw	613:614	Mw	613:614	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	50	theme	BPSs	712:715	arg1	characterization					669:684	preliminary structural characterization	646:684	preliminary structural characterization	646:684	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	50	theme	BPSs	712:715	arg1	yield					551:555	extraction yield	540:555	extraction yield	540:555	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	50	theme	BPSs	712:715	arg1	microstructure					690:703	microstructure	690:703	microstructure	690:703	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	0	51	theme	room	161:164	arg1	techniques					189:198	room temperature extraction techniques	161:198	room temperature extraction techniques	161:198	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	4	52	theme	acid-binding	1162:1173	arg1	capacity					1175:1182	the best bile acid-binding capacity	1148:1182	the best bile acid-binding capacity among the four BPSs	1148:1202	Moreover, BPS-A, which had the lowest Mws, showed the best bile acid-binding capacity among the four BPSs.					
32799166	3	53	contain	had	801:803	arg1	sample					728:733	The BPS-W sample	718:733	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water	718:799	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	53	contain	had	801:803	arg2	content					826:832	a higher uronic acid content	805:832	a higher uronic acid content (24.22%)	805:841	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	53	contain	had	801:803	arg2	%					840:840	24.22%	835:840	24.22%	835:840	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	4	54	theme	best	1152:1155	arg1	capacity					1175:1182	the best bile acid-binding capacity	1148:1182	the best bile acid-binding capacity among the four BPSs	1148:1202	Moreover, BPS-A, which had the lowest Mws, showed the best bile acid-binding capacity among the four BPSs.					
32799166	5	55	contain	had	1216:1218	arg1	study					1210:1214	This study	1205:1214	This study	1205:1214	This study had great potentials for the preparation of bioactive polysaccharides from fresh vegetables.					
32799166	5	55	contain	had	1216:1218	arg2	potentials					1226:1235	great potentials	1220:1235	great potentials for the preparation of bioactive polysaccharides from fresh vegetables	1220:1306	This study had great potentials for the preparation of bioactive polysaccharides from fresh vegetables.					
32799166	3	56	theme	higher	807:812	arg1	content					826:832	a higher uronic acid content	805:832	a higher uronic acid content (24.22%)	805:841	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	56	theme	higher	807:812	arg1	%					840:840	24.22%	835:840	24.22%	835:840	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	2	57	theme	extraction	488:497	arg1	methods					499:505	the extraction methods	484:505	the extraction methods	484:505	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	1	58	dep	obtained	244:251	arg1	Momordica					278:286	Momordica	278:286	Momordica	278:286	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	4	59	contain	had	1121:1123	arg1	BPS-A					1108:1112	BPS-A	1108:1112	BPS-A	1108:1112	Moreover, BPS-A, which had the lowest Mws, showed the best bile acid-binding capacity among the four BPSs.					
32799166	4	59	contain	had	1121:1123	arg2	Mws					1136:1138	the lowest Mws	1125:1138	the lowest Mws	1125:1138	Moreover, BPS-A, which had the lowest Mws, showed the best bile acid-binding capacity among the four BPSs.					
32799166	2	60	contain	had	507:509	arg2	influence					523:531	significant influence	511:531	significant influence	511:531	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	2	60	contain	had	507:509	arg1	methods					499:505	the extraction methods	484:505	the extraction methods	484:505	The results showed that the extraction methods had significant influence on the extraction yield, chemical composition, weight-average molecular weight (Mw), monosaccharide composition, preliminary structural characterization and microstructure of the BPSs.					
32799166	0	61	theme	biological	58:67	arg1	activities					69:78	in vitro biological activities	49:78	in vitro biological activities of polysaccharides	49:97	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	1	62	theme	room	305:308	arg1	extraction					410:419	ultrasonic-assisted extraction	390:419	ultrasonic-assisted extraction (UAE)	390:425	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	1	62	theme	room	305:308	arg1	techniques					333:342	room temperature extraction techniques	305:342	room temperature extraction techniques	305:342	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	1	62	theme	room	305:308	arg1	partitioning					367:378	three-phase partitioning	355:378	three-phase partitioning (TPP)	355:384	In this study, polysaccharides (BPSs) were obtained from fresh bitter gourd (Momordica charantia L.) by room temperature extraction techniques, including three-phase partitioning (TPP) and ultrasonic-assisted extraction (UAE) performed in different solvents.					
32799166	5	63	from	vegetables	1297:1306	arg1	polysaccharides					1270:1284	bioactive polysaccharides	1260:1284	bioactive polysaccharides from fresh vegetables	1260:1306	This study had great potentials for the preparation of bioactive polysaccharides from fresh vegetables.					
32799166	5	63	from	vegetables	1297:1306	arg1	preparation					1245:1255	the preparation	1241:1255	the preparation of bioactive polysaccharides from fresh vegetables	1241:1306	This study had great potentials for the preparation of bioactive polysaccharides from fresh vegetables.					
32799166	3	64	theme	citric	976:981	arg1	acid					983:986	citric acid	976:986	citric acid	976:986	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	0	65	dep	Momordica	133:141	arg1	L.					153:154	Momordica charantia L.	133:154	Momordica charantia L.	133:154	Production, physicochemical characteristics, and in vitro biological activities of polysaccharides obtained from fresh bitter gourd (Momordica charantia L.) via room temperature extraction techniques.					
32799166	3	66	theme	antioxidant	866:876	arg1	capacities					878:887	stronger antioxidant capacities	857:887	stronger antioxidant capacities	857:887	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
32799166	3	67	theme	BPS-W	722:726	arg1	sample					728:733	The BPS-W sample	718:733	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water	718:799	The BPS-W sample obtained from the bitter gourd residue via UAE in distilled water had a higher uronic acid content (24.22%) and possessed stronger antioxidant capacities and α-amylase and α-glycosidase inhibitory activities than BPS-C extracted with UAE in citric acid, BPS-A extracted with UAE in 1.25 mol/L NaOH/0.05% NaBH4, and BPS-J extracted from bitter gourd juice by TPP.					
33030182	0	0	theme	biological	80:89	arg1	properties					91:100	structural, mechanical, and biological properties	52:100	structural, mechanical, and biological properties	52:100	Nanofiber-reinforced bulk hydrogel: preparation and structural, mechanical, and biological properties.					
33030182	10	1	theme	hydrogel	1452:1459	arg1	surface					1461:1467	the composited hydrogel surface	1437:1467	the composited hydrogel surface	1437:1467	Compared with pure alginate hydrogel, the roughness of the composited hydrogel surface was increased, which resulted in stronger cell adhesion.					
33030182	1	2	theme	poor	246:249	arg1	strength					262:269	their poor mechanical strength	240:269	their poor mechanical strength	240:269	Alginate-based hydrogels are increasingly being used as biomaterials for tissue engineering, drug carriers, and wound dressing; however, their poor mechanical strength limits their applications.					
33030182	4	3	theme	nanofiber-reinforced	540:559	arg1	composites					584:593	nanofiber-reinforced bulk alginate hydrogel composites	540:593	nanofiber-reinforced bulk alginate hydrogel composites	540:593	Herein, we describe the fabrication of nanofiber-reinforced bulk alginate hydrogel composites.					
33030182	9	4	theme	sterile	1247:1253	arg1	environment					1255:1265	the sterile environment	1243:1265	the sterile environment	1243:1265	More importantly, the sterile environment continued to be maintained, and the composited hydrogel also had satisfactory cytocompatibility and cell adhesion.					
33030182	10	5	theme	pure	1396:1399	arg1	hydrogel					1410:1417	pure alginate hydrogel	1396:1417	pure alginate hydrogel	1396:1417	Compared with pure alginate hydrogel, the roughness of the composited hydrogel surface was increased, which resulted in stronger cell adhesion.					
33030182	2	6	theme	mechanical	364:373	arg1	strength					375:382	the mechanical strength	360:382	the mechanical strength of hydrogels	360:395	Nanofiber reinforcement is an effective method for increasing the mechanical strength of hydrogels.					
33030182	1	7	theme	mechanical	251:260	arg1	strength					262:269	their poor mechanical strength	240:269	their poor mechanical strength	240:269	Alginate-based hydrogels are increasingly being used as biomaterials for tissue engineering, drug carriers, and wound dressing; however, their poor mechanical strength limits their applications.					
33030182	7	8	theme	nanofiber	972:980	arg1	content					982:988	nanofiber content	972:988	nanofiber content	972:988	The compressive stress of the composite hydrogel exhibited "J-curve" behavior with gradually increasing nanofiber content, which indicated that the composited hydrogels were suitable as biomaterials.					
33030182	11	9	theme	composite	1541:1549	arg1	hydrogel					1551:1558	the composite hydrogel	1537:1558	the composite hydrogel	1537:1558	Therefore, the composite hydrogel demonstrated improved mechanical and biological properties, and exhibited the potential for clinical application.					
33030182	2	10	theme	Nanofiber	298:306	arg1	method					338:343	an effective method	325:343	an effective method for increasing the mechanical strength of hydrogels	325:395	Nanofiber reinforcement is an effective method for increasing the mechanical strength of hydrogels.					
33030182	2	10	theme	Nanofiber	298:306	arg1	reinforcement					308:320	Nanofiber reinforcement	298:320	Nanofiber reinforcement	298:320	Nanofiber reinforcement is an effective method for increasing the mechanical strength of hydrogels.					
33030182	7	11	theme	composite	898:906	arg1	hydrogel					908:915	the composite hydrogel	894:915	the composite hydrogel	894:915	The compressive stress of the composite hydrogel exhibited "J-curve" behavior with gradually increasing nanofiber content, which indicated that the composited hydrogels were suitable as biomaterials.					
33030182	2	12	theme	effective	328:336	arg1	method					338:343	an effective method	325:343	an effective method for increasing the mechanical strength of hydrogels	325:395	Nanofiber reinforcement is an effective method for increasing the mechanical strength of hydrogels.					
33030182	2	12	theme	effective	328:336	arg1	reinforcement					308:320	Nanofiber reinforcement	298:320	Nanofiber reinforcement	298:320	Nanofiber reinforcement is an effective method for increasing the mechanical strength of hydrogels.					
33030182	3	13	with	preparation	417:427	arg1	structure					475:483	a bulk structure	468:483	a bulk structure	468:483	However, the macro preparation of nanofiber-reinforced hydrogels with a bulk structure is challenging.					
33030182	8	14	theme	bacteriostatic	1167:1180	arg1	rate					1182:1185	the bacteriostatic rate	1163:1185	the bacteriostatic rate	1163:1185	Furthermore, in 2 h, the hydrogels killed more than 90% of the bacteria that were present, and the bacteriostatic rate reached 100% after 12 h of treatment.					
33030182	5	15	theme	reinforcement	672:684	arg1	law					686:688	the reinforcement law	668:688	the reinforcement law of nanofiber	668:701	The mechanical properties of hydrogels were significantly improved, and the reinforcement law of nanofiber was systematically studied.					
33030182	1	16	theme	drug	196:199	arg1	carriers					201:208	drug carriers	196:208	drug carriers	196:208	Alginate-based hydrogels are increasingly being used as biomaterials for tissue engineering, drug carriers, and wound dressing; however, their poor mechanical strength limits their applications.					
33030182	11	17	theme	improved	1573:1580	arg1	properties					1608:1617	improved mechanical and biological properties	1573:1617	improved mechanical and biological properties	1573:1617	Therefore, the composite hydrogel demonstrated improved mechanical and biological properties, and exhibited the potential for clinical application.					
33030182	3	18	theme	bulk	470:473	arg1	structure					475:483	a bulk structure	468:483	a bulk structure	468:483	However, the macro preparation of nanofiber-reinforced hydrogels with a bulk structure is challenging.					
33030182	0	19	theme	bulk	21:24	arg1	hydrogel					26:33	Nanofiber-reinforced bulk hydrogel	0:33	Nanofiber-reinforced bulk hydrogel: preparation and structural, mechanical, and biological properties.	0:101	Nanofiber-reinforced bulk hydrogel: preparation and structural, mechanical, and biological properties.					
33030182	9	20	theme	composited	1303:1312	arg1	hydrogel					1314:1321	the composited hydrogel	1299:1321	the composited hydrogel	1299:1321	More importantly, the sterile environment continued to be maintained, and the composited hydrogel also had satisfactory cytocompatibility and cell adhesion.					
33030182	10	21	theme	surface	1461:1467	arg1	roughness					1424:1432	the roughness	1420:1432	the roughness of the composited hydrogel surface	1420:1467	Compared with pure alginate hydrogel, the roughness of the composited hydrogel surface was increased, which resulted in stronger cell adhesion.					
33030182	5	22	theme	hydrogels	625:633	arg1	properties					611:620	The mechanical properties	596:620	The mechanical properties of hydrogels	596:633	The mechanical properties of hydrogels were significantly improved, and the reinforcement law of nanofiber was systematically studied.					
33030182	1	23	used	used	151:154	arg2	hydrogels					118:126	Alginate-based hydrogels	103:126	Alginate-based hydrogels	103:126	Alginate-based hydrogels are increasingly being used as biomaterials for tissue engineering, drug carriers, and wound dressing; however, their poor mechanical strength limits their applications.					
33030182	1	23	used	used	151:154	arg2	biomaterials					159:170	biomaterials	159:170	biomaterials for tissue engineering, drug carriers, and wound dressing	159:228	Alginate-based hydrogels are increasingly being used as biomaterials for tissue engineering, drug carriers, and wound dressing; however, their poor mechanical strength limits their applications.					
33030182	7	24	theme	compressive	872:882	arg1	stress					884:889	The compressive stress	868:889	The compressive stress of the composite hydrogel	868:915	The compressive stress of the composite hydrogel exhibited "J-curve" behavior with gradually increasing nanofiber content, which indicated that the composited hydrogels were suitable as biomaterials.					
33030182	3	25	theme	hydrogels	453:461	arg1	preparation					417:427	the macro preparation	407:427	the macro preparation of nanofiber-reinforced hydrogels with a bulk structure	407:483	However, the macro preparation of nanofiber-reinforced hydrogels with a bulk structure is challenging.					
33030182	0	26	theme	Nanofiber-reinforced	0:19	arg1	hydrogel					26:33	Nanofiber-reinforced bulk hydrogel	0:33	Nanofiber-reinforced bulk hydrogel: preparation and structural, mechanical, and biological properties.	0:101	Nanofiber-reinforced bulk hydrogel: preparation and structural, mechanical, and biological properties.					
33030182	5	27	theme	nanofiber	693:701	arg1	law					686:688	the reinforcement law	668:688	the reinforcement law of nanofiber	668:701	The mechanical properties of hydrogels were significantly improved, and the reinforcement law of nanofiber was systematically studied.					
33030182	9	28	theme	cell	1367:1370	arg1	adhesion					1372:1379	cell adhesion	1367:1379	cell adhesion	1367:1379	More importantly, the sterile environment continued to be maintained, and the composited hydrogel also had satisfactory cytocompatibility and cell adhesion.					
33030182	11	29	theme	mechanical	1582:1591	arg1	properties					1608:1617	improved mechanical and biological properties	1573:1617	improved mechanical and biological properties	1573:1617	Therefore, the composite hydrogel demonstrated improved mechanical and biological properties, and exhibited the potential for clinical application.					
33030182	10	30	theme	alginate	1401:1408	arg1	hydrogel					1410:1417	pure alginate hydrogel	1396:1417	pure alginate hydrogel	1396:1417	Compared with pure alginate hydrogel, the roughness of the composited hydrogel surface was increased, which resulted in stronger cell adhesion.					
33030182	1	31	theme	Alginate-based	103:116	arg1	biomaterials					159:170	biomaterials	159:170	biomaterials for tissue engineering, drug carriers, and wound dressing	159:228	Alginate-based hydrogels are increasingly being used as biomaterials for tissue engineering, drug carriers, and wound dressing; however, their poor mechanical strength limits their applications.					
33030182	1	31	theme	Alginate-based	103:116	arg1	hydrogels					118:126	Alginate-based hydrogels	103:126	Alginate-based hydrogels	103:126	Alginate-based hydrogels are increasingly being used as biomaterials for tissue engineering, drug carriers, and wound dressing; however, their poor mechanical strength limits their applications.					
33030182	6	32	theme	tensile	743:749	arg1	stress					751:756	The maximum tensile stress	731:756	The maximum tensile stress (0.76 MPa)	731:767	The maximum tensile stress (0.76 MPa) was obtained with 30% nanofiber content, which was 87% higher than that of pure alginate hydrogel.					
33030182	6	32	theme	tensile	743:749	arg1	MPa					764:766	0.76 MPa	759:766	0.76 MPa	759:766	The maximum tensile stress (0.76 MPa) was obtained with 30% nanofiber content, which was 87% higher than that of pure alginate hydrogel.					
33030182	6	33	theme	nanofiber	791:799	arg1	content					801:807	30% nanofiber content	787:807	30% nanofiber content	787:807	The maximum tensile stress (0.76 MPa) was obtained with 30% nanofiber content, which was 87% higher than that of pure alginate hydrogel.					
33030182	1	34	theme	tissue	176:181	arg1	engineering					183:193	tissue engineering	176:193	tissue engineering	176:193	Alginate-based hydrogels are increasingly being used as biomaterials for tissue engineering, drug carriers, and wound dressing; however, their poor mechanical strength limits their applications.					
33030182	6	35	theme	maximum	735:741	arg1	stress					751:756	The maximum tensile stress	731:756	The maximum tensile stress (0.76 MPa)	731:767	The maximum tensile stress (0.76 MPa) was obtained with 30% nanofiber content, which was 87% higher than that of pure alginate hydrogel.					
33030182	6	35	theme	maximum	735:741	arg1	MPa					764:766	0.76 MPa	759:766	0.76 MPa	759:766	The maximum tensile stress (0.76 MPa) was obtained with 30% nanofiber content, which was 87% higher than that of pure alginate hydrogel.					
33030182	9	36	theme	satisfactory	1332:1343	arg1	cytocompatibility					1345:1361	satisfactory cytocompatibility	1332:1361	satisfactory cytocompatibility	1332:1361	More importantly, the sterile environment continued to be maintained, and the composited hydrogel also had satisfactory cytocompatibility and cell adhesion.					
33030182	1	37	theme	wound	215:219	arg1	dressing					221:228	wound dressing	215:228	wound dressing	215:228	Alginate-based hydrogels are increasingly being used as biomaterials for tissue engineering, drug carriers, and wound dressing; however, their poor mechanical strength limits their applications.					
33030182	3	38	theme	nanofiber-reinforced	432:451	arg1	hydrogels					453:461	nanofiber-reinforced hydrogels	432:461	nanofiber-reinforced hydrogels	432:461	However, the macro preparation of nanofiber-reinforced hydrogels with a bulk structure is challenging.					
33030182	4	39	theme	alginate	566:573	arg1	composites					584:593	nanofiber-reinforced bulk alginate hydrogel composites	540:593	nanofiber-reinforced bulk alginate hydrogel composites	540:593	Herein, we describe the fabrication of nanofiber-reinforced bulk alginate hydrogel composites.					
33030182	11	40	theme	clinical	1652:1659	arg1	application					1661:1671	clinical application	1652:1671	clinical application	1652:1671	Therefore, the composite hydrogel demonstrated improved mechanical and biological properties, and exhibited the potential for clinical application.					
33030182	7	41	theme	composited	1016:1025	arg1	suitable					1042:1049	suitable	1042:1049	suitable	1042:1049	The compressive stress of the composite hydrogel exhibited "J-curve" behavior with gradually increasing nanofiber content, which indicated that the composited hydrogels were suitable as biomaterials.					
33030182	7	41	theme	composited	1016:1025	arg1	hydrogels					1027:1035	the composited hydrogels	1012:1035	the composited hydrogels	1012:1035	The compressive stress of the composite hydrogel exhibited "J-curve" behavior with gradually increasing nanofiber content, which indicated that the composited hydrogels were suitable as biomaterials.					
33030182	3	42	theme	macro	411:415	arg1	preparation					417:427	the macro preparation	407:427	the macro preparation of nanofiber-reinforced hydrogels with a bulk structure	407:483	However, the macro preparation of nanofiber-reinforced hydrogels with a bulk structure is challenging.					
33030182	4	43	theme	bulk	561:564	arg1	composites					584:593	nanofiber-reinforced bulk alginate hydrogel composites	540:593	nanofiber-reinforced bulk alginate hydrogel composites	540:593	Herein, we describe the fabrication of nanofiber-reinforced bulk alginate hydrogel composites.					
33030182	8	44	theme	bacteria	1131:1138	arg1	%					1122:1122	more than 90%	1110:1122	more than 90% of the bacteria that were present	1110:1156	Furthermore, in 2 h, the hydrogels killed more than 90% of the bacteria that were present, and the bacteriostatic rate reached 100% after 12 h of treatment.					
33030182	8	44	theme	bacteria	1131:1138	arg1	bacteria					1131:1138	the bacteria	1127:1138	the bacteria that were present	1127:1156	Furthermore, in 2 h, the hydrogels killed more than 90% of the bacteria that were present, and the bacteriostatic rate reached 100% after 12 h of treatment.					
33030182	8	44	theme	bacteria	1131:1138	arg1	present					1150:1156	present	1150:1156	present	1150:1156	Furthermore, in 2 h, the hydrogels killed more than 90% of the bacteria that were present, and the bacteriostatic rate reached 100% after 12 h of treatment.					
33030182	9	45	contain	had	1328:1330	arg2	adhesion					1372:1379	cell adhesion	1367:1379	cell adhesion	1367:1379	More importantly, the sterile environment continued to be maintained, and the composited hydrogel also had satisfactory cytocompatibility and cell adhesion.					
33030182	9	45	contain	had	1328:1330	arg1	hydrogel					1314:1321	the composited hydrogel	1299:1321	the composited hydrogel	1299:1321	More importantly, the sterile environment continued to be maintained, and the composited hydrogel also had satisfactory cytocompatibility and cell adhesion.					
33030182	9	45	contain	had	1328:1330	arg2	cytocompatibility					1345:1361	satisfactory cytocompatibility	1332:1361	satisfactory cytocompatibility	1332:1361	More importantly, the sterile environment continued to be maintained, and the composited hydrogel also had satisfactory cytocompatibility and cell adhesion.					
33030182	0	46	theme	structural	52:61	arg1	properties					91:100	structural, mechanical, and biological properties	52:100	structural, mechanical, and biological properties	52:100	Nanofiber-reinforced bulk hydrogel: preparation and structural, mechanical, and biological properties.					
33030182	4	47	theme	composites	584:593	arg1	fabrication					525:535	the fabrication	521:535	the fabrication of nanofiber-reinforced bulk alginate hydrogel composites	521:593	Herein, we describe the fabrication of nanofiber-reinforced bulk alginate hydrogel composites.					
33030182	5	48	theme	mechanical	600:609	arg1	properties					611:620	The mechanical properties	596:620	The mechanical properties of hydrogels	596:633	The mechanical properties of hydrogels were significantly improved, and the reinforcement law of nanofiber was systematically studied.					
33030182	7	49	theme	"	935:935	arg1	behavior					937:944	"J-curve" behavior	927:944	"J-curve" behavior	927:944	The compressive stress of the composite hydrogel exhibited "J-curve" behavior with gradually increasing nanofiber content, which indicated that the composited hydrogels were suitable as biomaterials.					
33030182	4	50	theme	hydrogel	575:582	arg1	composites					584:593	nanofiber-reinforced bulk alginate hydrogel composites	540:593	nanofiber-reinforced bulk alginate hydrogel composites	540:593	Herein, we describe the fabrication of nanofiber-reinforced bulk alginate hydrogel composites.					
33030182	10	51	theme	cell	1511:1514	arg1	adhesion					1516:1523	stronger cell adhesion	1502:1523	stronger cell adhesion	1502:1523	Compared with pure alginate hydrogel, the roughness of the composited hydrogel surface was increased, which resulted in stronger cell adhesion.					
33030182	6	52	theme	%	789:789	arg1	content					801:807	30% nanofiber content	787:807	30% nanofiber content	787:807	The maximum tensile stress (0.76 MPa) was obtained with 30% nanofiber content, which was 87% higher than that of pure alginate hydrogel.					
33030182	10	53	theme	stronger	1502:1509	arg1	adhesion					1516:1523	stronger cell adhesion	1502:1523	stronger cell adhesion	1502:1523	Compared with pure alginate hydrogel, the roughness of the composited hydrogel surface was increased, which resulted in stronger cell adhesion.					
33030182	6	54	theme	alginate	849:856	arg1	hydrogel					858:865	pure alginate hydrogel	844:865	pure alginate hydrogel	844:865	The maximum tensile stress (0.76 MPa) was obtained with 30% nanofiber content, which was 87% higher than that of pure alginate hydrogel.					
33030182	0	55	dep	hydrogel	26:33	arg1	properties					91:100	structural, mechanical, and biological properties	52:100	structural, mechanical, and biological properties	52:100	Nanofiber-reinforced bulk hydrogel: preparation and structural, mechanical, and biological properties.					
33030182	0	55	dep	hydrogel	26:33	arg1	preparation					36:46	preparation	36:46	preparation	36:46	Nanofiber-reinforced bulk hydrogel: preparation and structural, mechanical, and biological properties.					
33030182	6	56	theme	30	787:788	arg1	%					789:789	%	789:789	%	789:789	The maximum tensile stress (0.76 MPa) was obtained with 30% nanofiber content, which was 87% higher than that of pure alginate hydrogel.					
33030182	6	57	theme	pure	844:847	arg1	hydrogel					858:865	pure alginate hydrogel	844:865	pure alginate hydrogel	844:865	The maximum tensile stress (0.76 MPa) was obtained with 30% nanofiber content, which was 87% higher than that of pure alginate hydrogel.					
33030182	2	58	theme	hydrogels	387:395	arg1	strength					375:382	the mechanical strength	360:382	the mechanical strength of hydrogels	360:395	Nanofiber reinforcement is an effective method for increasing the mechanical strength of hydrogels.					
33030182	7	59	theme	J-curve	928:934	arg1	behavior					937:944	"J-curve" behavior	927:944	"J-curve" behavior	927:944	The compressive stress of the composite hydrogel exhibited "J-curve" behavior with gradually increasing nanofiber content, which indicated that the composited hydrogels were suitable as biomaterials.					
33030182	11	60	theme	biological	1597:1606	arg1	properties					1608:1617	improved mechanical and biological properties	1573:1617	improved mechanical and biological properties	1573:1617	Therefore, the composite hydrogel demonstrated improved mechanical and biological properties, and exhibited the potential for clinical application.					
33030182	0	61	theme	mechanical	64:73	arg1	properties					91:100	structural, mechanical, and biological properties	52:100	structural, mechanical, and biological properties	52:100	Nanofiber-reinforced bulk hydrogel: preparation and structural, mechanical, and biological properties.					
33030182	8	62	theme	treatment	1214:1222	arg1	h					1209:1209	12 h	1206:1209	12 h of treatment	1206:1222	Furthermore, in 2 h, the hydrogels killed more than 90% of the bacteria that were present, and the bacteriostatic rate reached 100% after 12 h of treatment.					
33030182	10	63	theme	composited	1441:1450	arg1	surface					1461:1467	the composited hydrogel surface	1437:1467	the composited hydrogel surface	1437:1467	Compared with pure alginate hydrogel, the roughness of the composited hydrogel surface was increased, which resulted in stronger cell adhesion.					
33030182	7	64	theme	hydrogel	908:915	arg1	stress					884:889	The compressive stress	868:889	The compressive stress of the composite hydrogel	868:915	The compressive stress of the composite hydrogel exhibited "J-curve" behavior with gradually increasing nanofiber content, which indicated that the composited hydrogels were suitable as biomaterials.					
34375000	9	0	theme	lectins	1195:1201	arg1	capacity					1164:1171	The capacity	1160:1171	The capacity of CLEC4G and mCD209c lectins to block SARS-CoV-2 viral entry	1160:1233	The capacity of CLEC4G and mCD209c lectins to block SARS-CoV-2 viral entry holds promise for pan-variant therapeutic interventions.					
34375000	3	1	theme	comprehensive	341:353	arg1	library					355:361	a comprehensive library	339:361	a comprehensive library of mammalian carbohydrate-binding proteins (lectins)	339:414	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	2	from	residues	440:447	arg1	RBD					516:518	RBD	516:518	RBD	516:518	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	2	from	residues	440:447	arg1	SARS-CoV-2					524:533	SARS-CoV-2	524:533	SARS-CoV-2	524:533	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	2	from	residues	440:447	arg1	domain					508:513	the receptor binding domain	487:513	the receptor binding domain (RBD) of SARS-CoV-2	487:533	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	2	from	residues	440:447	arg1	Spike					477:481	the full-length trimeric Spike	452:481	the full-length trimeric Spike	452:481	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	6	3	theme	RBD-ACE2	823:830	arg1	interface					832:840	the RBD-ACE2 interface	819:840	the RBD-ACE2 interface	819:840	3D modelling showed that both lectins can bind to a glycan within the RBD-ACE2 interface and thus interferes with Spike binding to cell surfaces.					
34375000	3	4	theme	SARS-CoV-2	524:533	arg1	RBD					516:518	RBD	516:518	RBD	516:518	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	4	theme	SARS-CoV-2	524:533	arg1	SARS-CoV-2					524:533	SARS-CoV-2	524:533	SARS-CoV-2	524:533	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	4	theme	SARS-CoV-2	524:533	arg1	domain					508:513	the receptor binding domain	487:513	the receptor binding domain (RBD) of SARS-CoV-2	487:533	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	4	theme	SARS-CoV-2	524:533	arg1	Spike					477:481	the full-length trimeric Spike	452:481	the full-length trimeric Spike	452:481	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	9	5	theme	pan-variant	1253:1263	arg1	interventions					1277:1289	pan-variant therapeutic interventions	1253:1289	pan-variant therapeutic interventions	1253:1289	The capacity of CLEC4G and mCD209c lectins to block SARS-CoV-2 viral entry holds promise for pan-variant therapeutic interventions.					
34375000	3	6	theme	proteins	397:404	arg1	library					355:361	a comprehensive library	339:361	a comprehensive library of mammalian carbohydrate-binding proteins (lectins)	339:414	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	8	7	theme	lectin-Spike	1048:1059	arg1	interactions					1061:1072	lectin-Spike interactions	1048:1072	lectin-Spike interactions	1048:1072	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	8	8	theme	first	997:1001	arg1	map					1013:1015	first extensive map	997:1015	first extensive map	997:1015	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	5	9	theme	force	735:739	arg1	microscopy					741:750	atomic force microscopy	728:750	atomic force microscopy	728:750	Clec4g and CD209c binding to Spike was dissected and visualized in real time and at single-molecule resolution using atomic force microscopy.					
34375000	5	10	theme	single-molecule	695:709	arg1	resolution					711:720	single-molecule resolution	695:720	single-molecule resolution	695:720	Clec4g and CD209c binding to Spike was dissected and visualized in real time and at single-molecule resolution using atomic force microscopy.					
34375000	7	11	theme	SARS-CoV-2	952:961	arg1	infections					963:972	SARS-CoV-2 infections	952:972	SARS-CoV-2 infections	952:972	Importantly, Clec4g and CD209c significantly reduced SARS-CoV-2 infections.					
34375000	2	12	theme	therapeutic	300:310	arg1	intervention					312:323	robust therapeutic intervention	293:323	robust therapeutic intervention	293:323	The 22 N-glycan sites of Spike remain highly conserved among SARS-CoV-2 variants, opening an avenue for robust therapeutic intervention.					
34375000	0	13	theme	glycosylation	60:72	arg1	sites					74:78	conserved SARS-CoV-2 glycosylation sites	39:78	conserved SARS-CoV-2 glycosylation sites	39:78	Identification of lectin receptors for conserved SARS-CoV-2 glycosylation sites.					
34375000	6	14	theme	cell	884:887	arg1	surfaces					889:896	cell surfaces	884:896	cell surfaces	884:896	3D modelling showed that both lectins can bind to a glycan within the RBD-ACE2 interface and thus interferes with Spike binding to cell surfaces.					
34375000	9	15	theme	viral	1223:1227	arg1	entry					1229:1233	SARS-CoV-2 viral entry	1212:1233	SARS-CoV-2 viral entry	1212:1233	The capacity of CLEC4G and mCD209c lectins to block SARS-CoV-2 viral entry holds promise for pan-variant therapeutic interventions.					
34375000	1	16	theme	New	81:83	arg1	variants					96:103	New SARS-CoV-2 variants	81:103	New SARS-CoV-2 variants	81:103	New SARS-CoV-2 variants are continuously emerging with critical implications for therapies or vaccinations.					
34375000	3	17	theme	trimeric	468:475	arg1	Spike					477:481	the full-length trimeric Spike	452:481	the full-length trimeric Spike	452:481	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	18	theme	sugar	434:438	arg1	residues					440:447	critical sugar residues	425:447	critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2	425:533	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	8	19	theme	interactions	1061:1072	arg1	map					1013:1015	first extensive map	997:1015	first extensive map	997:1015	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	8	19	theme	interactions	1061:1072	arg1	modelling					1035:1043	3D structural modelling	1021:1043	3D structural modelling	1021:1043	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	9	20	theme	therapeutic	1265:1275	arg1	interventions					1277:1289	pan-variant therapeutic interventions	1253:1289	pan-variant therapeutic interventions	1253:1289	The capacity of CLEC4G and mCD209c lectins to block SARS-CoV-2 viral entry holds promise for pan-variant therapeutic interventions.					
34375000	1	21	theme	SARS-CoV-2	85:94	arg1	variants					96:103	New SARS-CoV-2 variants	81:103	New SARS-CoV-2 variants	81:103	New SARS-CoV-2 variants are continuously emerging with critical implications for therapies or vaccinations.					
34375000	8	22	dep	map	1013:1015	arg1	the					993:995	the	993:995	the	993:995	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	3	23	theme	mammalian	366:374	arg1	lectins					407:413	lectins	407:413	lectins	407:413	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	23	theme	mammalian	366:374	arg1	proteins					397:404	mammalian carbohydrate-binding proteins	366:404	mammalian carbohydrate-binding proteins (lectins)	366:414	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	8	24	theme	candidate	1087:1095	arg1	receptors					1097:1105	candidate receptors	1087:1105	candidate receptors involved in Spike binding and SARS-CoV-2 infections	1087:1157	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	8	25	theme	extensive	1003:1011	arg1	map					1013:1015	first extensive map	997:1015	first extensive map	997:1015	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	8	26	theme	3D	1021:1022	arg1	modelling					1035:1043	3D structural modelling	1021:1043	3D structural modelling	1021:1043	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	3	27	theme	full-length	456:466	arg1	Spike					477:481	the full-length trimeric Spike	452:481	the full-length trimeric Spike	452:481	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	0	28	theme	receptors	25:33	arg1	Identification					0:13	Identification	0:13	Identification of lectin receptors for conserved SARS-CoV-2 glycosylation sites.	0:79	Identification of lectin receptors for conserved SARS-CoV-2 glycosylation sites.					
34375000	3	29	theme	receptor	491:498	arg1	RBD					516:518	RBD	516:518	RBD	516:518	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	29	theme	receptor	491:498	arg1	SARS-CoV-2					524:533	SARS-CoV-2	524:533	SARS-CoV-2	524:533	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	29	theme	receptor	491:498	arg1	domain					508:513	the receptor binding domain	487:513	the receptor binding domain (RBD) of SARS-CoV-2	487:533	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	0	30	theme	lectin	18:23	arg1	receptors					25:33	lectin receptors	18:33	lectin receptors	18:33	Identification of lectin receptors for conserved SARS-CoV-2 glycosylation sites.					
34375000	2	31	theme	robust	293:298	arg1	intervention					312:323	robust therapeutic intervention	293:323	robust therapeutic intervention	293:323	The 22 N-glycan sites of Spike remain highly conserved among SARS-CoV-2 variants, opening an avenue for robust therapeutic intervention.					
34375000	6	32	theme	Spike	867:871	arg1	binding					873:879	Spike binding	867:879	Spike binding to cell surfaces	867:896	3D modelling showed that both lectins can bind to a glycan within the RBD-ACE2 interface and thus interferes with Spike binding to cell surfaces.					
34375000	3	33	theme	binding	500:506	arg1	RBD					516:518	RBD	516:518	RBD	516:518	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	33	theme	binding	500:506	arg1	SARS-CoV-2					524:533	SARS-CoV-2	524:533	SARS-CoV-2	524:533	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	33	theme	binding	500:506	arg1	domain					508:513	the receptor binding domain	487:513	the receptor binding domain (RBD) of SARS-CoV-2	487:533	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	0	34	theme	conserved	39:47	arg1	sites					74:78	conserved SARS-CoV-2 glycosylation sites	39:78	conserved SARS-CoV-2 glycosylation sites	39:78	Identification of lectin receptors for conserved SARS-CoV-2 glycosylation sites.					
34375000	8	35	theme	Spike	1119:1123	arg1	binding					1125:1131	Spike binding	1119:1131	Spike binding	1119:1131	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	2	36	theme	N-glycan	196:203	arg1	Spike					214:218	Spike	214:218	Spike	214:218	The 22 N-glycan sites of Spike remain highly conserved among SARS-CoV-2 variants, opening an avenue for robust therapeutic intervention.					
34375000	2	36	theme	N-glycan	196:203	arg1	sites					205:209	The 22 N-glycan sites	189:209	The 22 N-glycan sites of Spike	189:218	The 22 N-glycan sites of Spike remain highly conserved among SARS-CoV-2 variants, opening an avenue for robust therapeutic intervention.					
34375000	2	37	theme	SARS-CoV-2	250:259	arg1	variants					261:268	SARS-CoV-2 variants	250:268	SARS-CoV-2 variants	250:268	The 22 N-glycan sites of Spike remain highly conserved among SARS-CoV-2 variants, opening an avenue for robust therapeutic intervention.					
34375000	3	38	theme	carbohydrate-binding	376:395	arg1	lectins					407:413	lectins	407:413	lectins	407:413	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	38	theme	carbohydrate-binding	376:395	arg1	proteins					397:404	mammalian carbohydrate-binding proteins	366:404	mammalian carbohydrate-binding proteins (lectins)	366:414	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	3	39	used	used	334:337	arg2	we					331:332	we	331:332	we	331:332	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	5	40	theme	atomic	728:733	arg1	microscopy					741:750	atomic force microscopy	728:750	atomic force microscopy	728:750	Clec4g and CD209c binding to Spike was dissected and visualized in real time and at single-molecule resolution using atomic force microscopy.					
34375000	9	41	theme	SARS-CoV-2	1212:1221	arg1	entry					1229:1233	SARS-CoV-2 viral entry	1212:1233	SARS-CoV-2 viral entry	1212:1233	The capacity of CLEC4G and mCD209c lectins to block SARS-CoV-2 viral entry holds promise for pan-variant therapeutic interventions.					
34375000	0	42	theme	SARS-CoV-2	49:58	arg1	sites					74:78	conserved SARS-CoV-2 glycosylation sites	39:78	conserved SARS-CoV-2 glycosylation sites	39:78	Identification of lectin receptors for conserved SARS-CoV-2 glycosylation sites.					
34375000	5	43	theme	real	678:681	arg1	time					683:686	real time	678:686	real time	678:686	Clec4g and CD209c binding to Spike was dissected and visualized in real time and at single-molecule resolution using atomic force microscopy.					
34375000	1	44	theme	critical	136:143	arg1	implications					145:156	critical implications	136:156	critical implications for therapies or vaccinations	136:186	New SARS-CoV-2 variants are continuously emerging with critical implications for therapies or vaccinations.					
34375000	6	45	theme	3D	753:754	arg1	modelling					756:764	3D modelling	753:764	3D modelling	753:764	3D modelling showed that both lectins can bind to a glycan within the RBD-ACE2 interface and thus interferes with Spike binding to cell surfaces.					
34375000	8	46	theme	SARS-CoV-2	1137:1146	arg1	infections					1148:1157	SARS-CoV-2 infections	1137:1157	SARS-CoV-2 infections	1137:1157	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	2	47	theme	Spike	214:218	arg1	Spike					214:218	Spike	214:218	Spike	214:218	The 22 N-glycan sites of Spike remain highly conserved among SARS-CoV-2 variants, opening an avenue for robust therapeutic intervention.					
34375000	2	47	theme	Spike	214:218	arg1	sites					205:209	The 22 N-glycan sites	189:209	The 22 N-glycan sites of Spike	189:218	The 22 N-glycan sites of Spike remain highly conserved among SARS-CoV-2 variants, opening an avenue for robust therapeutic intervention.					
34375000	9	48	theme	CLEC4G	1176:1181	arg1	lectins					1195:1201	CLEC4G and mCD209c lectins	1176:1201	CLEC4G and mCD209c lectins	1176:1201	The capacity of CLEC4G and mCD209c lectins to block SARS-CoV-2 viral entry holds promise for pan-variant therapeutic interventions.					
34375000	8	49	theme	structural	1024:1033	arg1	modelling					1035:1043	3D structural modelling	1021:1043	3D structural modelling	1021:1043	These data report the first extensive map and 3D structural modelling of lectin-Spike interactions and uncovers candidate receptors involved in Spike binding and SARS-CoV-2 infections.					
34375000	3	50	theme	critical	425:432	arg1	residues					440:447	critical sugar residues	425:447	critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2	425:533	Here we used a comprehensive library of mammalian carbohydrate-binding proteins (lectins) to probe critical sugar residues on the full-length trimeric Spike and the receptor binding domain (RBD) of SARS-CoV-2.					
34375000	0	51	gly	glycosylation	60:72	arg2	sites					74:78	conserved SARS-CoV-2 glycosylation sites	39:78	conserved SARS-CoV-2 glycosylation sites	39:78	Identification of lectin receptors for conserved SARS-CoV-2 glycosylation sites.					
34375000	9	52	theme	mCD209c	1187:1193	arg1	lectins					1195:1201	CLEC4G and mCD209c lectins	1176:1201	CLEC4G and mCD209c lectins	1176:1201	The capacity of CLEC4G and mCD209c lectins to block SARS-CoV-2 viral entry holds promise for pan-variant therapeutic interventions.					
32126202	1	0	theme	ginger	325:330	arg1	polysaccharides					332:346	crude ginger polysaccharides	319:346	extract crude ginger polysaccharides (GPs)	311:352	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	0	theme	ginger	325:330	arg1	GPs					349:351	GPs	349:351	GPs	349:351	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	5	1	theme	glycosidic	848:857	arg1	linkage					859:865	the glycosidic linkage	844:865	the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→	844:899	Mannose and glucose as the main monosaccharide and the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→ existed in both five GPs.					
32126202	0	2	theme	Zingiber	83:90	arg1	ginger					75:80	ginger	75:80	ginger (Zingiber officinale)	75:102	Characterization and antitumor activities of polysaccharides obtained from ginger (Zingiber officinale) by different extraction methods.					
32126202	0	2	theme	Zingiber	83:90	arg1	officinale					92:101	Zingiber officinale	83:101	Zingiber officinale	83:101	Characterization and antitumor activities of polysaccharides obtained from ginger (Zingiber officinale) by different extraction methods.					
32126202	6	3	theme	UGP1	1000:1003	arg1	structure					966:974	specific structure	957:974	specific structure of →6)-β-D-Galp-(1→ and UGP1	957:1003	While EGP2 and UGP1 possessed specific structure of →6)-β-D-Galp-(1→ and UGP1 contained more sulfate group.					
32126202	1	4	theme	hot	187:189	arg1	HWE					209:211	HWE	209:211	HWE	209:211	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	4	theme	hot	187:189	arg1	extraction					197:206	hot water extraction	187:206	hot water extraction (HWE)	187:212	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	5	theme	ultrasonic	252:261	arg1	UCGE					288:291	UCGE	288:291	UCGE	288:291	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	5	theme	ultrasonic	252:261	arg1	extraction					276:285	ultrasonic cell grinder extraction	252:285	ultrasonic cell grinder extraction (UCGE)	252:292	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	4	6	from	different	735:743	arg1	GPs					753:755	five GPs	748:755	five GPs	748:755	The molecular weights were different in five GPs, varying from 11.81 to 1831.75 kDa.					
32126202	6	7	theme	specific	957:964	arg1	structure					966:974	specific structure	957:974	specific structure of →6)-β-D-Galp-(1→ and UGP1	957:1003	While EGP2 and UGP1 possessed specific structure of →6)-β-D-Galp-(1→ and UGP1 contained more sulfate group.					
32126202	1	8	theme	cell	263:266	arg1	UCGE					288:291	UCGE	288:291	UCGE	288:291	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	8	theme	cell	263:266	arg1	extraction					276:285	ultrasonic cell grinder extraction	252:285	ultrasonic cell grinder extraction (UCGE)	252:292	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	2	9	theme	GPs	513:515	arg1	UGP2					555:558	UGP2	555:558	UGP2	555:558	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	2	9	theme	GPs	513:515	arg1	fractions					517:525	Five GPs fractions	508:525	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively)	508:573	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	2	9	theme	GPs	513:515	arg1	EGP2					539:542	EGP2	539:542	EGP2	539:542	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	2	9	theme	GPs	513:515	arg1	UGP1					545:548	UGP1	545:548	UGP1	545:548	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	2	9	theme	GPs	513:515	arg1	HGP					528:530	HGP	528:530	HGP	528:530	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	2	9	theme	GPs	513:515	arg1	EGP1					533:536	EGP1	533:536	EGP1	533:536	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	6	10	contain	possessed	947:955	arg1	EGP2					933:936	EGP2	933:936	EGP2	933:936	While EGP2 and UGP1 possessed specific structure of →6)-β-D-Galp-(1→ and UGP1 contained more sulfate group.					
32126202	6	10	contain	possessed	947:955	arg1	UGP1					942:945	UGP1	942:945	UGP1	942:945	While EGP2 and UGP1 possessed specific structure of →6)-β-D-Galp-(1→ and UGP1 contained more sulfate group.					
32126202	6	10	contain	possessed	947:955	arg2	structure					966:974	specific structure	957:974	specific structure of →6)-β-D-Galp-(1→ and UGP1	957:1003	While EGP2 and UGP1 possessed specific structure of →6)-β-D-Galp-(1→ and UGP1 contained more sulfate group.					
32126202	5	11	dep	-α-D-Glc	873:880	arg1	→4					870:871	→4	870:871	→4	870:871	Mannose and glucose as the main monosaccharide and the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→ existed in both five GPs.					
32126202	1	12	theme	DEAE	426:429	arg1	cellulose-52					431:442	DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography	426:491	cellulose-52	431:442	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	5	13	theme	1→	898:899	arg1	linkage					859:865	the glycosidic linkage	844:865	the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→	844:899	Mannose and glucose as the main monosaccharide and the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→ existed in both five GPs.					
32126202	5	13	theme	1→	898:899	arg1	monosaccharide					825:838	the main monosaccharide	816:838	the main monosaccharide	816:838	Mannose and glucose as the main monosaccharide and the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→ existed in both five GPs.					
32126202	1	14	theme	grinder	268:274	arg1	UCGE					288:291	UCGE	288:291	UCGE	288:291	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	14	theme	grinder	268:274	arg1	extraction					276:285	ultrasonic cell grinder extraction	252:285	ultrasonic cell grinder extraction (UCGE)	252:292	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	0	15	theme	extraction	117:126	arg1	methods					128:134	different extraction methods	107:134	different extraction methods	107:134	Characterization and antitumor activities of polysaccharides obtained from ginger (Zingiber officinale) by different extraction methods.					
32126202	1	16	dep	extract	311:317	arg1	polysaccharides					332:346	crude ginger polysaccharides	319:346	extract crude ginger polysaccharides (GPs)	311:352	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	16	dep	extract	311:317	arg1	GPs					349:351	GPs	349:351	GPs	349:351	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	17	theme	water	191:195	arg1	HWE					209:211	HWE	209:211	HWE	209:211	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	17	theme	water	191:195	arg1	extraction					197:206	hot water extraction	187:206	hot water extraction (HWE)	187:212	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	0	18	theme	different	107:115	arg1	methods					128:134	different extraction methods	107:134	different extraction methods	107:134	Characterization and antitumor activities of polysaccharides obtained from ginger (Zingiber officinale) by different extraction methods.					
32126202	3	19	theme	antitumor	664:672	arg1	activities					674:683	antitumor activities	664:683	antitumor activities	664:683	The differences of five GPs in chemical composition, characterization and antitumor activities were further compared.					
32126202	3	20	from	differences	594:604	arg1	characterization					643:658	characterization	643:658	characterization	643:658	The differences of five GPs in chemical composition, characterization and antitumor activities were further compared.					
32126202	3	20	from	differences	594:604	arg1	activities					674:683	antitumor activities	664:683	antitumor activities	664:683	The differences of five GPs in chemical composition, characterization and antitumor activities were further compared.					
32126202	3	20	from	differences	594:604	arg1	composition					630:640	chemical composition	621:640	chemical composition	621:640	The differences of five GPs in chemical composition, characterization and antitumor activities were further compared.					
32126202	7	21	theme	colon	1121:1125	arg1	cancer					1127:1132	the colon cancer	1117:1132	the colon cancer	1117:1132	Moreover, UGP1 exhibited strong inhibitory effect on three tumor cells especially the colon cancer.					
32126202	1	22	theme	Sephadex	448:455	arg1	chromatography					478:491	DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography	426:491	chromatography	478:491	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	5	23	theme	main	820:823	arg1	monosaccharide					825:838	the main monosaccharide	816:838	the main monosaccharide	816:838	Mannose and glucose as the main monosaccharide and the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→ existed in both five GPs.					
32126202	1	24	theme	G-200	457:461	arg1	chromatography					478:491	DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography	426:491	chromatography	478:491	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	7	25	theme	strong	1060:1065	arg1	effect					1078:1083	strong inhibitory effect	1060:1083	strong inhibitory effect	1060:1083	Moreover, UGP1 exhibited strong inhibitory effect on three tumor cells especially the colon cancer.					
32126202	1	26	theme	size-exclusion	463:476	arg1	chromatography					478:491	DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography	426:491	chromatography	478:491	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	4	27	theme	molecular	712:720	arg1	different					735:743	different	735:743	different	735:743	The molecular weights were different in five GPs, varying from 11.81 to 1831.75 kDa.					
32126202	4	27	theme	molecular	712:720	arg1	weights					722:728	The molecular weights	708:728	The molecular weights	708:728	The molecular weights were different in five GPs, varying from 11.81 to 1831.75 kDa.					
32126202	9	28	theme	active	1324:1329	arg1	structure					1331:1339	more active structure	1319:1339	more active structure	1319:1339	The study indicated GPs extracted by UCGE could reserve more active structure and inhibit colon cancer more significantly.					
32126202	4	29	dep	1831.75 kDa	780:790	arg1	to					777:778	to	777:778	to	777:778	The molecular weights were different in five GPs, varying from 11.81 to 1831.75 kDa.					
32126202	0	30	theme	antitumor	21:29	arg1	activities					31:40	antitumor activities	21:40	antitumor activities	21:40	Characterization and antitumor activities of polysaccharides obtained from ginger (Zingiber officinale) by different extraction methods.					
32126202	1	31	theme	respective	366:375	arg1	parameters					382:391	best parameters	377:391	their respective best parameters	360:391	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	5	32	theme	-α-Manp-	889:896	arg1	1→					898:899	-α-Manp-(1→	889:899	-α-Manp-(1→	889:899	Mannose and glucose as the main monosaccharide and the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→ existed in both five GPs.					
32126202	8	33	theme	UGP1	1159:1162	arg1	rates					1150:1154	The inhibition rates	1135:1154	The inhibition rates of UGP1 on H1975, HCT116 and MCF-7	1135:1189	The inhibition rates of UGP1 on H1975, HCT116 and MCF-7 were 23.339 ± 2.285%, 56.843 ± 2.405% and 21.061 ± 1.920% respectively.					
32126202	8	33	theme	UGP1	1159:1162	arg1	%					1210:1210	23.339 ± 2.285%	1196:1210	23.339 ± 2.285%	1196:1210	The inhibition rates of UGP1 on H1975, HCT116 and MCF-7 were 23.339 ± 2.285%, 56.843 ± 2.405% and 21.061 ± 1.920% respectively.					
32126202	0	34	theme	polysaccharides	45:59	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and antitumor activities of polysaccharides obtained from ginger (Zingiber officinale) by different extraction methods.					
32126202	0	34	theme	polysaccharides	45:59	arg1	activities					31:40	antitumor activities	21:40	antitumor activities	21:40	Characterization and antitumor activities of polysaccharides obtained from ginger (Zingiber officinale) by different extraction methods.					
32126202	2	35	dep	fractions	517:525	arg1	UGP2					555:558	UGP2	555:558	UGP2	555:558	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	2	35	dep	fractions	517:525	arg1	fractions					517:525	Five GPs fractions	508:525	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively)	508:573	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	2	35	dep	fractions	517:525	arg1	EGP2					539:542	EGP2	539:542	EGP2	539:542	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	2	35	dep	fractions	517:525	arg1	UGP1					545:548	UGP1	545:548	UGP1	545:548	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	2	35	dep	fractions	517:525	arg1	HGP					528:530	HGP	528:530	HGP	528:530	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	2	35	dep	fractions	517:525	arg1	EGP1					533:536	EGP1	533:536	EGP1	533:536	Five GPs fractions (HGP, EGP1, EGP2, UGP1, and UGP2, respectively) were obtained.					
32126202	1	36	theme	best	377:380	arg1	parameters					382:391	best parameters	377:391	their respective best parameters	360:391	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	37	theme	enzyme	215:220	arg1	EAE					243:245	EAE	243:245	EAE	243:245	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	37	theme	enzyme	215:220	arg1	extraction					231:240	enzyme assisted extraction	215:240	enzyme assisted extraction (EAE)	215:246	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	8	38	theme	inhibition	1139:1148	arg1	rates					1150:1154	The inhibition rates	1135:1154	The inhibition rates of UGP1 on H1975, HCT116 and MCF-7	1135:1189	The inhibition rates of UGP1 on H1975, HCT116 and MCF-7 were 23.339 ± 2.285%, 56.843 ± 2.405% and 21.061 ± 1.920% respectively.					
32126202	8	38	theme	inhibition	1139:1148	arg1	%					1210:1210	23.339 ± 2.285%	1196:1210	23.339 ± 2.285%	1196:1210	The inhibition rates of UGP1 on H1975, HCT116 and MCF-7 were 23.339 ± 2.285%, 56.843 ± 2.405% and 21.061 ± 1.920% respectively.					
32126202	9	39	dep	indicated	1273:1281	arg1	inhibit					1345:1351	inhibit	1345:1351	inhibit colon cancer more significantly	1345:1383	The study indicated GPs extracted by UCGE could reserve more active structure and inhibit colon cancer more significantly.					
32126202	9	39	dep	indicated	1273:1281	arg1	reserve					1311:1317	reserve	1311:1317	reserve	1311:1317	The study indicated GPs extracted by UCGE could reserve more active structure and inhibit colon cancer more significantly.					
32126202	1	40	theme	assisted	222:229	arg1	EAE					243:245	EAE	243:245	EAE	243:245	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	40	theme	assisted	222:229	arg1	extraction					231:240	enzyme assisted extraction	215:240	enzyme assisted extraction (EAE)	215:246	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	8	41	from	rates	1150:1154	arg1	MCF-7					1185:1189	MCF-7	1185:1189	MCF-7	1185:1189	The inhibition rates of UGP1 on H1975, HCT116 and MCF-7 were 23.339 ± 2.285%, 56.843 ± 2.405% and 21.061 ± 1.920% respectively.					
32126202	8	41	from	rates	1150:1154	arg1	HCT116					1174:1179	HCT116	1174:1179	HCT116	1174:1179	The inhibition rates of UGP1 on H1975, HCT116 and MCF-7 were 23.339 ± 2.285%, 56.843 ± 2.405% and 21.061 ± 1.920% respectively.					
32126202	8	41	from	rates	1150:1154	arg1	H1975					1167:1171	H1975	1167:1171	H1975	1167:1171	The inhibition rates of UGP1 on H1975, HCT116 and MCF-7 were 23.339 ± 2.285%, 56.843 ± 2.405% and 21.061 ± 1.920% respectively.					
32126202	7	42	theme	tumor	1094:1098	arg1	cells					1100:1104	three tumor cells	1088:1104	three tumor cells	1088:1104	Moreover, UGP1 exhibited strong inhibitory effect on three tumor cells especially the colon cancer.					
32126202	6	43	dep	-β-D-Galp-	982:991	arg1	→6					979:980	→6	979:980	→6	979:980	While EGP2 and UGP1 possessed specific structure of →6)-β-D-Galp-(1→ and UGP1 contained more sulfate group.					
32126202	3	44	theme	chemical	621:628	arg1	composition					630:640	chemical composition	621:640	chemical composition	621:640	The differences of five GPs in chemical composition, characterization and antitumor activities were further compared.					
32126202	1	45	theme	crude	399:403	arg1	GPs					405:407	crude GPs	399:407	crude GPs	399:407	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	6	46	theme	sulfate	1020:1026	arg1	group					1028:1032	more sulfate group	1015:1032	more sulfate group	1015:1032	While EGP2 and UGP1 possessed specific structure of →6)-β-D-Galp-(1→ and UGP1 contained more sulfate group.					
32126202	4	47	from	GPs	753:755	arg1	different					735:743	different	735:743	different	735:743	The molecular weights were different in five GPs, varying from 11.81 to 1831.75 kDa.					
32126202	4	47	from	GPs	753:755	arg1	weights					722:728	The molecular weights	708:728	The molecular weights	708:728	The molecular weights were different in five GPs, varying from 11.81 to 1831.75 kDa.					
32126202	1	48	theme	different	143:151	arg1	technologies					164:175	Three different extraction technologies	137:175	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE)	137:292	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	48	theme	different	143:151	arg1	extraction					276:285	ultrasonic cell grinder extraction	252:285	ultrasonic cell grinder extraction (UCGE)	252:292	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	48	theme	different	143:151	arg1	extraction					231:240	enzyme assisted extraction	215:240	enzyme assisted extraction (EAE)	215:246	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	48	theme	different	143:151	arg1	extraction					197:206	hot water extraction	187:206	hot water extraction (HWE)	187:212	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	5	49	theme	-α-D-Glc	873:880	arg1	linkage					859:865	the glycosidic linkage	844:865	the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→	844:899	Mannose and glucose as the main monosaccharide and the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→ existed in both five GPs.					
32126202	5	49	theme	-α-D-Glc	873:880	arg1	monosaccharide					825:838	the main monosaccharide	816:838	the main monosaccharide	816:838	Mannose and glucose as the main monosaccharide and the glycosidic linkage of →4)-α-D-Glc(1→ and -α-Manp-(1→ existed in both five GPs.					
32126202	3	50	theme	GPs	614:616	arg1	differences					594:604	The differences	590:604	The differences of five GPs in chemical composition, characterization and antitumor activities	590:683	The differences of five GPs in chemical composition, characterization and antitumor activities were further compared.					
32126202	9	51	theme	colon	1353:1357	arg1	cancer					1359:1364	colon cancer	1353:1364	colon cancer	1353:1364	The study indicated GPs extracted by UCGE could reserve more active structure and inhibit colon cancer more significantly.					
32126202	1	52	theme	extraction	153:162	arg1	technologies					164:175	Three different extraction technologies	137:175	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE)	137:292	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	52	theme	extraction	153:162	arg1	extraction					276:285	ultrasonic cell grinder extraction	252:285	ultrasonic cell grinder extraction (UCGE)	252:292	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	52	theme	extraction	153:162	arg1	extraction					231:240	enzyme assisted extraction	215:240	enzyme assisted extraction (EAE)	215:246	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	52	theme	extraction	153:162	arg1	extraction					197:206	hot water extraction	187:206	hot water extraction (HWE)	187:212	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	6	53	theme	-β-D-Galp-	982:991	arg1	structure					966:974	specific structure	957:974	specific structure of →6)-β-D-Galp-(1→ and UGP1	957:1003	While EGP2 and UGP1 possessed specific structure of →6)-β-D-Galp-(1→ and UGP1 contained more sulfate group.					
32126202	1	54	theme	crude	319:323	arg1	polysaccharides					332:346	crude ginger polysaccharides	319:346	extract crude ginger polysaccharides (GPs)	311:352	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	54	theme	crude	319:323	arg1	GPs					349:351	GPs	349:351	GPs	349:351	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	7	55	theme	inhibitory	1067:1076	arg1	effect					1078:1083	strong inhibitory effect	1060:1083	strong inhibitory effect	1060:1083	Moreover, UGP1 exhibited strong inhibitory effect on three tumor cells especially the colon cancer.					
32126202	1	56	from	chromatography	478:491	arg1	order					501:505	that order	496:505	that order	496:505	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
32126202	1	57	from	cellulose-52	431:442	arg1	order					501:505	that order	496:505	that order	496:505	Three different extraction technologies including hot water extraction (HWE), enzyme assisted extraction (EAE) and ultrasonic cell grinder extraction (UCGE) were employed to extract crude ginger polysaccharides (GPs) under their respective best parameters, then crude GPs were purified by DEAE cellulose-52 and Sephadex G-200 size-exclusion chromatography in that order.					
33436255	0	0	theme	structural	102:111	arg1	characterization					113:128	structural characterization	102:128	structural characterization	102:128	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.					
33436255	7	1	dep	provides	1094:1101	arg1	developing					1227:1236	developing	1227:1236	developing the application in food and biomedicine industries	1227:1287	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
33436255	7	1	dep	provides	1094:1101	arg1	guiding					1189:1195	guiding	1189:1195	guiding its biological researches	1189:1221	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
33436255	1	2	theme	polysaccharides	181:195	arg1	source					164:169	a common source	155:169	a common source of active polysaccharides	155:195	Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated.					
33436255	1	2	theme	polysaccharides	181:195	arg1	cylindracea					140:150	Agrocybe cylindracea	131:150	Agrocybe cylindracea	131:150	Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated.					
33436255	0	3	from	evaluation	14:23	arg1	cylindracea					75:85	Agrocybe cylindracea	66:85	Agrocybe cylindracea	66:85	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.					
33436255	3	4	theme	purified	504:511	arg1	fractions					513:521	the purified fractions	500:521	the purified fractions' physicochemical properties	500:549	Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied.					
33436255	5	5	theme	β-glucopyranosyl	879:894	arg1	residues					896:903	terminal and 3-substituted β-glucopyranosyl residues	852:903	residues	896:903	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	6	6	theme	4:1	1079:1081	arg1	ratio					1070:1074	a ratio	1068:1074	a ratio of 4:1	1068:1081	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	4	7	theme	main	749:752	arg1	chain					754:758	main chain	749:758	main chain	749:758	JACP-30 was identified as a fucoglucogalactan with a α-(1 → 6)-galactopyranosyl as main chain.					
33436255	0	8	from	cylindracea	75:85	arg1	evaluation					14:23	Comprehensive evaluation	0:23	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.	0:129	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.					
33436255	0	8	from	cylindracea	75:85	arg1	polysaccharides					45:59	alkali-extracted polysaccharides	28:59	alkali-extracted polysaccharides from Agrocybe cylindracea	28:85	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.					
33436255	6	9	dep	-linked	995:1001	arg1	→					991:991	1 → 6	989:993	1 → 6	989:993	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	6	10	theme	-linked	1033:1039	arg1	residues					1056:1063	-linked glucopyranosyl residues	1033:1063	-linked glucopyranosyl residues	1033:1063	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	6	11	theme	JACP-80	974:980	arg1	backbone					962:969	the backbone	958:969	the backbone of JACP-80	958:980	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	6	11	theme	JACP-80	974:980	arg1	glucopyranosyl					1003:1016	β-(1 → 6)-linked glucopyranosyl	986:1016	β-(1 → 6)-linked glucopyranosyl	986:1016	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	6	12	link	-linked	995:1001	arg1	backbone					962:969	the backbone	958:969	the backbone of JACP-80	958:980	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	6	12	link	-linked	995:1001	arg1	glucopyranosyl					1003:1016	β-(1 → 6)-linked glucopyranosyl	986:1016	β-(1 → 6)-linked glucopyranosyl	986:1016	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	5	13	dep	β-	805:806	arg1	→					810:810	1 → 6	808:812	1 → 6	808:812	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	3	14	theme	physicochemical	524:538	arg1	viscosities					610:620	viscosities	610:620	viscosities	610:620	Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied.					
33436255	3	14	theme	physicochemical	524:538	arg1	morphology					634:643	surface morphology	626:643	surface morphology	626:643	Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied.					
33436255	3	14	theme	physicochemical	524:538	arg1	weights					601:607	molecular weights	591:607	molecular weights	591:607	Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied.					
33436255	3	14	theme	physicochemical	524:538	arg1	properties					540:549	the purified fractions' physicochemical properties	500:549	the purified fractions' physicochemical properties	500:549	Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied.					
33436255	3	14	theme	physicochemical	524:538	arg1	compositions					577:588	monosaccharide compositions	562:588	monosaccharide compositions	562:588	Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied.					
33436255	5	15	theme	terminal	852:859	arg1	residues					896:903	terminal and 3-substituted β-glucopyranosyl residues	852:903	residues	896:903	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	5	16	attach	attached	905:912	arg3	O-3					917:919	O-3	917:919	O-3 for every three residues	917:944	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	5	16	attach	attached	905:912	arg2	residues					896:903	terminal and 3-substituted β-glucopyranosyl residues	852:903	residues	896:903	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	3	17	dep	showed	493:498	arg1	varied					658:663	varied	658:663	showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied	493:663	Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied.					
33436255	2	18	theme	A.	332:333	arg1	JACP					348:351	JACP	348:351	JACP	348:351	In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC-MS, and 1D/2D NMR analysis.					
33436255	2	18	theme	A.	332:333	arg1	cylindracea					335:345	A. cylindracea	332:345	A. cylindracea (JACP)	332:352	In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC-MS, and 1D/2D NMR analysis.					
33436255	1	19	theme	fine	208:211	arg1	structures					213:222	their fine structures	202:222	their fine structures	202:222	Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated.					
33436255	0	20	theme	Comprehensive	0:12	arg1	evaluation					14:23	Comprehensive evaluation	0:23	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.	0:129	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.					
33436255	3	21	theme	molecular	591:599	arg1	weights					601:607	molecular weights	591:607	molecular weights	591:607	Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied.					
33436255	7	22	from	understanding	1127:1139	arg1	cylindracea					1168:1178	A. cylindracea	1165:1178	A. cylindracea	1165:1178	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
33436255	0	23	theme	alkali-extracted	28:43	arg1	polysaccharides					45:59	alkali-extracted polysaccharides	28:59	alkali-extracted polysaccharides from Agrocybe cylindracea	28:85	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.					
33436255	3	24	theme	surface	626:632	arg1	morphology					634:643	surface morphology	626:643	surface morphology	626:643	Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied.					
33436255	3	25	theme	monosaccharide	562:575	arg1	compositions					577:588	monosaccharide compositions	562:588	monosaccharide compositions	562:588	Results showed the purified fractions' physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied.					
33436255	7	26	theme	food	1257:1260	arg1	industries					1278:1287	food and biomedicine industries	1257:1287	food and biomedicine industries	1257:1287	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
33436255	7	27	theme	more	1103:1106	arg1	information					1108:1118	more information	1103:1118	more information to the understanding of polysaccharides from A. cylindracea	1103:1178	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
33436255	2	28	theme	alkaline	312:319	arg1	extract					321:327	the alkaline extract	308:327	the alkaline extract of A. cylindracea (JACP)	308:352	In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC-MS, and 1D/2D NMR analysis.					
33436255	4	29	theme	α-	719:720	arg1	→					724:724	1 → 6	722:726	1 → 6	722:726	JACP-30 was identified as a fucoglucogalactan with a α-(1 → 6)-galactopyranosyl as main chain.					
33436255	4	29	theme	α-	719:720	arg1	-galactopyranosyl					728:744	a α-(1 → 6)-galactopyranosyl	717:744	a α-(1 → 6)-galactopyranosyl as main chain	717:758	JACP-30 was identified as a fucoglucogalactan with a α-(1 → 6)-galactopyranosyl as main chain.					
33436255	2	30	theme	NMR	471:473	arg1	analysis					475:482	1D/2D NMR analysis	465:482	1D/2D NMR analysis	465:482	In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC-MS, and 1D/2D NMR analysis.					
33436255	5	31	with	-glucans	814:821	arg1	chains					833:838	side chains	828:838	side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues	828:944	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	4	32	with	fucoglucogalactan	694:710	arg1	→					724:724	1 → 6	722:726	1 → 6	722:726	JACP-30 was identified as a fucoglucogalactan with a α-(1 → 6)-galactopyranosyl as main chain.					
33436255	4	32	with	fucoglucogalactan	694:710	arg1	-galactopyranosyl					728:744	a α-(1 → 6)-galactopyranosyl	717:744	a α-(1 → 6)-galactopyranosyl as main chain	717:758	JACP-30 was identified as a fucoglucogalactan with a α-(1 → 6)-galactopyranosyl as main chain.					
33436255	2	33	theme	present	259:265	arg1	study					267:271	the present study	255:271	the present study	255:271	In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC-MS, and 1D/2D NMR analysis.					
33436255	5	34	theme	side	828:831	arg1	chains					833:838	side chains	828:838	side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues	828:944	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	0	35	theme	polysaccharides	45:59	arg1	evaluation					14:23	Comprehensive evaluation	0:23	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.	0:129	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.					
33436255	2	36	theme	1D/2D	465:469	arg1	analysis					475:482	1D/2D NMR analysis	465:482	1D/2D NMR analysis	465:482	In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC-MS, and 1D/2D NMR analysis.					
33436255	0	37	from	Comparison	88:97	arg1	characterization					113:128	structural characterization	102:128	structural characterization	102:128	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.					
33436255	7	38	from	application	1242:1252	arg1	industries					1278:1287	food and biomedicine industries	1257:1287	food and biomedicine industries	1257:1287	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
33436255	6	39	theme	-linked	995:1001	arg1	backbone					962:969	the backbone	958:969	the backbone of JACP-80	958:980	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	6	39	theme	-linked	995:1001	arg1	glucopyranosyl					1003:1016	β-(1 → 6)-linked glucopyranosyl	986:1016	β-(1 → 6)-linked glucopyranosyl	986:1016	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	5	40	theme	β-	805:806	arg1	-glucans					814:821	β-(1 → 6)-glucans	805:821	β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues	805:944	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	5	40	theme	β-	805:806	arg1	JACP-80r					774:781	JACP-80r	774:781	JACP-80r	774:781	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	5	40	theme	β-	805:806	arg1	JACP-50p					761:768	JACP-50p	761:768	JACP-50p	761:768	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	5	41	theme	3-substituted	865:877	arg1	residues					896:903	terminal and 3-substituted β-glucopyranosyl residues	852:903	residues	896:903	JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O-3 for every three residues.					
33436255	0	42	theme	Agrocybe	66:73	arg1	cylindracea					75:85	Agrocybe cylindracea	66:85	Agrocybe cylindracea	66:85	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.					
33436255	6	43	link	-linked	1033:1039	arg1	residues					1056:1063	-linked glucopyranosyl residues	1033:1063	-linked glucopyranosyl residues	1033:1063	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	7	44	theme	biological	1201:1210	arg1	researches					1212:1221	its biological researches	1197:1221	its biological researches	1197:1221	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
33436255	6	45	from	ratio	1070:1074	arg1	backbone					962:969	the backbone	958:969	the backbone of JACP-80	958:980	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	6	45	from	ratio	1070:1074	arg1	β-					1022:1023	β-	1022:1023	β-(1 → 3,6)	1022:1032	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	6	45	from	ratio	1070:1074	arg1	→					1027:1027	1 → 3,6	1025:1031	1 → 3,6	1025:1031	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	6	45	from	ratio	1070:1074	arg1	glucopyranosyl					1003:1016	β-(1 → 6)-linked glucopyranosyl	986:1016	β-(1 → 6)-linked glucopyranosyl	986:1016	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	1	46	theme	common	157:162	arg1	source					164:169	a common source	155:169	a common source of active polysaccharides	155:195	Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated.					
33436255	1	46	theme	common	157:162	arg1	cylindracea					140:150	Agrocybe cylindracea	131:150	Agrocybe cylindracea	131:150	Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated.					
33436255	1	47	theme	Agrocybe	131:138	arg1	source					164:169	a common source	155:169	a common source of active polysaccharides	155:195	Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated.					
33436255	1	47	theme	Agrocybe	131:138	arg1	cylindracea					140:150	Agrocybe cylindracea	131:150	Agrocybe cylindracea	131:150	Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated.					
33436255	7	48	from	cylindracea	1168:1178	arg1	polysaccharides					1144:1158	polysaccharides	1144:1158	polysaccharides from A. cylindracea	1144:1178	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
33436255	7	48	from	cylindracea	1168:1178	arg1	understanding					1127:1139	the understanding	1123:1139	the understanding of polysaccharides from A. cylindracea	1123:1178	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
33436255	6	49	dep	glucopyranosyl	1003:1016	arg1	residues					1056:1063	-linked glucopyranosyl residues	1033:1063	-linked glucopyranosyl residues	1033:1063	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	2	50	theme	cylindracea	335:345	arg1	extract					321:327	the alkaline extract	308:327	the alkaline extract of A. cylindracea (JACP)	308:352	In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC-MS, and 1D/2D NMR analysis.					
33436255	7	51	theme	biomedicine	1266:1276	arg1	industries					1278:1287	food and biomedicine industries	1257:1287	food and biomedicine industries	1257:1287	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
33436255	6	52	theme	glucopyranosyl	1041:1054	arg1	residues					1056:1063	-linked glucopyranosyl residues	1033:1063	-linked glucopyranosyl residues	1033:1063	Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1.					
33436255	0	53	dep	evaluation	14:23	arg1	Comparison					88:97	Comparison	88:97	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.	0:129	Comprehensive evaluation of alkali-extracted polysaccharides from Agrocybe cylindracea: Comparison on structural characterization.					
33436255	2	54	theme	chemical	365:372	arg1	components					374:383	their chemical components	359:383	their chemical components	359:383	In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC-MS, and 1D/2D NMR analysis.					
33436255	1	55	theme	active	174:179	arg1	polysaccharides					181:195	active polysaccharides	174:195	active polysaccharides	174:195	Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated.					
33436255	7	56	theme	polysaccharides	1144:1158	arg1	understanding					1127:1139	the understanding	1123:1139	the understanding of polysaccharides from A. cylindracea	1123:1178	This work provides more information to the understanding of polysaccharides from A. cylindracea, further guiding its biological researches and developing the application in food and biomedicine industries.					
32721822	0	0	theme	silk	90:93	arg1	sericin					95:101	silk sericin	90:101	silk sericin	90:101	Production and characterization of bacterial cellulose membranes with hyaluronic acid and silk sericin.					
32721822	6	1	theme	Thermogravimetric	823:839	arg1	analysis					841:848	Thermogravimetric analysis	823:848	Thermogravimetric analysis	823:848	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	8	2	theme	BC	1303:1304	arg1	materials					1434:1442	cosmetic materials	1425:1442	cosmetic materials	1425:1442	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	2	theme	BC	1303:1304	arg1	composites					1312:1321	the novel BC based composites	1293:1321	the novel BC based composites especially the ex situ modified BC-HA/SS composite	1293:1372	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	2	theme	BC	1303:1304	arg1	dressing					1412:1419	wound dressing	1406:1419	wound dressing	1406:1419	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	6	3	theme	composite	917:925	arg1	better					931:936	better	931:936	better	931:936	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	3	theme	composite	917:925	arg1	stability					874:882	the thermal stability	862:882	the thermal stability of the ex situ modified BC-HA/SS composite	862:925	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	4	theme	in	959:960	arg1	composite					985:993	in situ modified BC-HA/SS composite	959:993	in situ modified BC-HA/SS composite	959:993	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	7	5	located	observed	1202:1209	arg2	viability					1188:1196	improved cell viability	1174:1196	improved cell viability	1174:1196	Further, improved cell viability was observed on the ex situ modified BC-HA/SS composite.					
32721822	7	5	located	observed	1202:1209	arg1	composite					1244:1252	the ex situ modified BC-HA/SS composite	1214:1252	the ex situ modified BC-HA/SS composite	1214:1252	Further, improved cell viability was observed on the ex situ modified BC-HA/SS composite.					
32721822	8	6	dep	ex	1338:1339	arg1	situ					1341:1344	situ	1341:1344	situ	1341:1344	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	6	7	theme	content	1030:1036	arg1	highest					1092:1098	highest	1092:1098	highest	1092:1098	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	7	theme	content	1030:1036	arg1	values					1038:1043	in situ modified BC-HA/SS composite, the tensile strength and moisture content values	959:1043	in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS	959:1076	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	1	8	theme	unique	241:246	arg1	structure					248:256	its unique structure	237:256	its unique structure	237:256	Bacterial cellulose (BC) is a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties.					
32721822	7	9	theme	BC-HA/SS	1235:1242	arg1	composite					1244:1252	the ex situ modified BC-HA/SS composite	1214:1252	the ex situ modified BC-HA/SS composite	1214:1252	Further, improved cell viability was observed on the ex situ modified BC-HA/SS composite.					
32721822	2	10	dep	in	369:370	arg1	situ					372:375	situ	372:375	situ	372:375	In this study, BC was functionalized with hyaluronic acid (HA) and silk sericin (SS) using the in situ and ex situ methods.					
32721822	4	11	theme	crystalline	673:683	arg1	form					685:688	a typical type I cellulose crystalline form	646:688	a typical type I cellulose crystalline form	646:688	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	6	12	theme	modified	899:906	arg1	composite					917:925	the ex situ modified BC-HA/SS composite	887:925	the ex situ modified BC-HA/SS composite	887:925	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	13	theme	modified	1146:1153	arg1	BC-HA/SS					1155:1162	in situ modified BC-HA/SS	1138:1162	in situ modified BC-HA/SS	1138:1162	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	4	14	theme	cellulose	663:671	arg1	form					685:688	a typical type I cellulose crystalline form	646:688	a typical type I cellulose crystalline form	646:688	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	7	15	theme	cell	1183:1186	arg1	viability					1188:1196	improved cell viability	1174:1196	improved cell viability	1174:1196	Further, improved cell viability was observed on the ex situ modified BC-HA/SS composite.					
32721822	4	16	contain	had	642:644	arg2	form					685:688	a typical type I cellulose crystalline form	646:688	a typical type I cellulose crystalline form	646:688	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	4	16	contain	had	642:644	arg1	composites					631:640	the novel BC-HA/SS composites	612:640	the novel BC-HA/SS composites	612:640	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	6	17	theme	BC-HA/SS	1069:1076	arg1	highest					1092:1098	highest	1092:1098	highest	1092:1098	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	17	theme	BC-HA/SS	1069:1076	arg1	values					1038:1043	in situ modified BC-HA/SS composite, the tensile strength and moisture content values	959:1043	in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS	959:1076	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	18	theme	modified	967:974	arg1	composite					985:993	in situ modified BC-HA/SS composite	959:993	in situ modified BC-HA/SS composite	959:993	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	19	theme	composite	985:993	arg1	highest					1092:1098	highest	1092:1098	highest	1092:1098	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	19	theme	composite	985:993	arg1	values					1038:1043	in situ modified BC-HA/SS composite, the tensile strength and moisture content values	959:1043	in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS	959:1076	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	8	20	theme	modified	1346:1353	arg1	composite					1364:1372	the ex situ modified BC-HA/SS composite	1334:1372	the novel BC based composites especially the ex situ modified BC-HA/SS composite	1293:1372	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	7	21	dep	ex	1218:1219	arg1	situ					1221:1224	situ	1221:1224	situ	1221:1224	Further, improved cell viability was observed on the ex situ modified BC-HA/SS composite.					
32721822	6	22	theme	in	1138:1139	arg1	BC-HA/SS					1155:1162	in situ modified BC-HA/SS	1138:1162	in situ modified BC-HA/SS	1138:1162	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	23	theme	modified	1060:1067	arg1	BC-HA/SS					1069:1076	the ex situ modified BC-HA/SS	1048:1076	the ex situ modified BC-HA/SS	1048:1076	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	4	24	theme	microscopy	572:581	arg1	analysis					589:596	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis	522:596	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis	522:596	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	6	25	dep	in	1138:1139	arg1	situ					1141:1144	situ	1141:1144	situ	1141:1144	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	8	26	theme	cosmetic	1425:1432	arg1	materials					1434:1442	cosmetic materials	1425:1442	cosmetic materials	1425:1442	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	26	theme	cosmetic	1425:1432	arg1	composites					1312:1321	the novel BC based composites	1293:1321	the novel BC based composites especially the ex situ modified BC-HA/SS composite	1293:1372	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	4	27	theme	typical	648:654	arg1	form					685:688	a typical type I cellulose crystalline form	646:688	a typical type I cellulose crystalline form	646:688	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	1	28	theme	increasing	173:182	arg1	interest					184:191	increasing interest	173:191	increasing interest in the biomedical and cosmetic field due to its unique structure and properties	173:271	Bacterial cellulose (BC) is a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties.					
32721822	2	29	theme	ex	381:382	arg1	methods					389:395	the in situ and ex situ methods	365:395	the in situ and ex situ methods	365:395	In this study, BC was functionalized with hyaluronic acid (HA) and silk sericin (SS) using the in situ and ex situ methods.					
32721822	6	30	theme	ex	891:892	arg1	composite					917:925	the ex situ modified BC-HA/SS composite	887:925	the ex situ modified BC-HA/SS composite	887:925	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	2	31	dep	ex	381:382	arg1	situ					384:387	situ	384:387	situ	384:387	In this study, BC was functionalized with hyaluronic acid (HA) and silk sericin (SS) using the in situ and ex situ methods.					
32721822	8	32	theme	BC-HA/SS	1355:1362	arg1	composite					1364:1372	the ex situ modified BC-HA/SS composite	1334:1372	the novel BC based composites especially the ex situ modified BC-HA/SS composite	1293:1372	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	6	33	theme	ex	1052:1053	arg1	BC-HA/SS					1069:1076	the ex situ modified BC-HA/SS	1048:1076	the ex situ modified BC-HA/SS	1048:1076	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	2	34	theme	silk	341:344	arg1	SS					355:356	SS	355:356	SS	355:356	In this study, BC was functionalized with hyaluronic acid (HA) and silk sericin (SS) using the in situ and ex situ methods.					
32721822	2	34	theme	silk	341:344	arg1	sericin					346:352	silk sericin	341:352	silk sericin (SS)	341:357	In this study, BC was functionalized with hyaluronic acid (HA) and silk sericin (SS) using the in situ and ex situ methods.					
32721822	5	35	theme	hybrid	759:764	arg1	membranes					766:774	the hybrid membranes	755:774	the hybrid membranes	755:774	The roughness of the hybrid membranes was analyzed by atomic force microscopy (AFM).					
32721822	7	36	theme	ex	1218:1219	arg1	composite					1244:1252	the ex situ modified BC-HA/SS composite	1214:1252	the ex situ modified BC-HA/SS composite	1214:1252	Further, improved cell viability was observed on the ex situ modified BC-HA/SS composite.					
32721822	6	37	dep	in	959:960	arg1	situ					962:965	situ	962:965	situ	962:965	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	1	38	theme	Bacterial	104:112	arg1	polysaccharide					142:155	a natural polysaccharide	132:155	a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties	132:271	Bacterial cellulose (BC) is a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties.					
32721822	1	38	theme	Bacterial	104:112	arg1	BC					125:126	BC	125:126	BC	125:126	Bacterial cellulose (BC) is a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties.					
32721822	1	38	theme	Bacterial	104:112	arg1	cellulose					114:122	Bacterial cellulose	104:122	Bacterial cellulose (BC)	104:127	Bacterial cellulose (BC) is a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties.					
32721822	2	39	theme	in	369:370	arg1	methods					389:395	the in situ and ex situ methods	365:395	the in situ and ex situ methods	365:395	In this study, BC was functionalized with hyaluronic acid (HA) and silk sericin (SS) using the in situ and ex situ methods.					
32721822	7	40	theme	improved	1174:1181	arg1	viability					1188:1196	improved cell viability	1174:1196	improved cell viability	1174:1196	Further, improved cell viability was observed on the ex situ modified BC-HA/SS composite.					
32721822	4	41	theme	diffraction	532:542	arg1	analysis					589:596	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis	522:596	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis	522:596	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	4	42	theme	type	656:659	arg1	form					685:688	a typical type I cellulose crystalline form	646:688	a typical type I cellulose crystalline form	646:688	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	6	43	theme	strength	1008:1015	arg1	highest					1092:1098	highest	1092:1098	highest	1092:1098	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	43	theme	strength	1008:1015	arg1	values					1038:1043	in situ modified BC-HA/SS composite, the tensile strength and moisture content values	959:1043	in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS	959:1076	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	0	44	theme	cellulose	45:53	arg1	membranes					55:63	bacterial cellulose membranes	35:63	bacterial cellulose membranes	35:63	Production and characterization of bacterial cellulose membranes with hyaluronic acid and silk sericin.					
32721822	4	45	theme	novel	616:620	arg1	composites					631:640	the novel BC-HA/SS composites	612:640	the novel BC-HA/SS composites	612:640	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	4	46	theme	electron	563:570	arg1	SEM					584:586	SEM	584:586	SEM	584:586	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	4	46	theme	electron	563:570	arg1	microscopy					572:581	scanning electron microscopy	554:581	scanning electron microscopy (SEM)	554:587	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	6	47	theme	pure	1126:1129	arg1	BC					1131:1132	the pure BC	1122:1132	the pure BC	1122:1132	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	5	48	theme	atomic	792:797	arg1	AFM					817:819	AFM	817:819	AFM	817:819	The roughness of the hybrid membranes was analyzed by atomic force microscopy (AFM).					
32721822	5	48	theme	atomic	792:797	arg1	microscopy					805:814	atomic force microscopy	792:814	atomic force microscopy (AFM)	792:820	The roughness of the hybrid membranes was analyzed by atomic force microscopy (AFM).					
32721822	1	49	theme	biomedical	200:209	arg1	field					224:228	the biomedical and cosmetic field	196:228	the biomedical and cosmetic field due to its unique structure and properties	196:271	Bacterial cellulose (BC) is a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties.					
32721822	0	50	theme	bacterial	35:43	arg1	membranes					55:63	bacterial cellulose membranes	35:63	bacterial cellulose membranes	35:63	Production and characterization of bacterial cellulose membranes with hyaluronic acid and silk sericin.					
32721822	6	51	theme	thermal	866:872	arg1	better					931:936	better	931:936	better	931:936	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	51	theme	thermal	866:872	arg1	stability					874:882	the thermal stability	862:882	the thermal stability of the ex situ modified BC-HA/SS composite	862:925	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	52	theme	BC-HA/SS	908:915	arg1	composite					917:925	the ex situ modified BC-HA/SS composite	887:925	the ex situ modified BC-HA/SS composite	887:925	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	6	53	dep	ex	1052:1053	arg1	situ					1055:1058	situ	1055:1058	situ	1055:1058	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	4	54	theme	scanning	554:561	arg1	SEM					584:586	SEM	584:586	SEM	584:586	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	4	54	theme	scanning	554:561	arg1	microscopy					572:581	scanning electron microscopy	554:581	scanning electron microscopy (SEM)	554:587	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	8	55	theme	wound	1406:1410	arg1	composites					1312:1321	the novel BC based composites	1293:1321	the novel BC based composites especially the ex situ modified BC-HA/SS composite	1293:1372	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	55	theme	wound	1406:1410	arg1	dressing					1412:1419	wound dressing	1406:1419	wound dressing	1406:1419	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	6	56	theme	pure	947:950	arg1	BC					952:953	the pure BC	943:953	the pure BC	943:953	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	3	57	theme	BC	509:510	arg1	membrane					512:519	the BC membrane	505:519	the BC membrane	505:519	Fourier transform infrared spectroscopy (FTIR) showed that the HA and SS were successfully integrated into the BC membrane.					
32721822	0	58	with	characterization	15:30	arg1	acid					81:84	hyaluronic acid	70:84	hyaluronic acid	70:84	Production and characterization of bacterial cellulose membranes with hyaluronic acid and silk sericin.					
32721822	0	58	with	characterization	15:30	arg1	sericin					95:101	silk sericin	90:101	silk sericin	90:101	Production and characterization of bacterial cellulose membranes with hyaluronic acid and silk sericin.					
32721822	4	59	theme	BC-HA/SS	622:629	arg1	composites					631:640	the novel BC-HA/SS composites	612:640	the novel BC-HA/SS composites	612:640	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	0	60	theme	membranes	55:63	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of bacterial cellulose membranes with hyaluronic acid and silk sericin.					
32721822	0	60	theme	membranes	55:63	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of bacterial cellulose membranes with hyaluronic acid and silk sericin.					
32721822	8	61	dep	composites	1312:1321	arg1	composite					1364:1372	the ex situ modified BC-HA/SS composite	1334:1372	the novel BC based composites especially the ex situ modified BC-HA/SS composite	1293:1372	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	62	theme	study	1275:1279	arg1	results					1259:1265	The results	1255:1265	The results of this study	1255:1279	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	2	63	theme	hyaluronic	316:325	arg1	HA					333:334	HA	333:334	HA	333:334	In this study, BC was functionalized with hyaluronic acid (HA) and silk sericin (SS) using the in situ and ex situ methods.					
32721822	2	63	theme	hyaluronic	316:325	arg1	acid					327:330	hyaluronic acid	316:330	hyaluronic acid (HA)	316:335	In this study, BC was functionalized with hyaluronic acid (HA) and silk sericin (SS) using the in situ and ex situ methods.					
32721822	8	64	theme	based	1306:1310	arg1	materials					1434:1442	cosmetic materials	1425:1442	cosmetic materials	1425:1442	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	64	theme	based	1306:1310	arg1	composites					1312:1321	the novel BC based composites	1293:1321	the novel BC based composites especially the ex situ modified BC-HA/SS composite	1293:1372	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	64	theme	based	1306:1310	arg1	dressing					1412:1419	wound dressing	1406:1419	wound dressing	1406:1419	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	65	theme	novel	1297:1301	arg1	materials					1434:1442	cosmetic materials	1425:1442	cosmetic materials	1425:1442	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	65	theme	novel	1297:1301	arg1	composites					1312:1321	the novel BC based composites	1293:1321	the novel BC based composites especially the ex situ modified BC-HA/SS composite	1293:1372	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	65	theme	novel	1297:1301	arg1	dressing					1412:1419	wound dressing	1406:1419	wound dressing	1406:1419	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	8	66	theme	ex	1338:1339	arg1	composite					1364:1372	the ex situ modified BC-HA/SS composite	1334:1372	the novel BC based composites especially the ex situ modified BC-HA/SS composite	1293:1372	The results of this study showed that the novel BC based composites especially the ex situ modified BC-HA/SS composite could be applied potentially as wound dressing and cosmetic materials.					
32721822	7	67	theme	modified	1226:1233	arg1	composite					1244:1252	the ex situ modified BC-HA/SS composite	1214:1252	the ex situ modified BC-HA/SS composite	1214:1252	Further, improved cell viability was observed on the ex situ modified BC-HA/SS composite.					
32721822	0	68	with	Production	0:9	arg1	acid					81:84	hyaluronic acid	70:84	hyaluronic acid	70:84	Production and characterization of bacterial cellulose membranes with hyaluronic acid and silk sericin.					
32721822	0	68	with	Production	0:9	arg1	sericin					95:101	silk sericin	90:101	silk sericin	90:101	Production and characterization of bacterial cellulose membranes with hyaluronic acid and silk sericin.					
32721822	6	69	theme	moisture	1021:1028	arg1	content					1030:1036	moisture content	1021:1036	moisture content	1021:1036	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	4	70	theme	I	661:661	arg1	form					685:688	a typical type I cellulose crystalline form	646:688	a typical type I cellulose crystalline form	646:688	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	1	71	theme	cosmetic	215:222	arg1	field					224:228	the biomedical and cosmetic field	196:228	the biomedical and cosmetic field due to its unique structure and properties	196:271	Bacterial cellulose (BC) is a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties.					
32721822	6	72	dep	ex	891:892	arg1	situ					894:897	situ	894:897	situ	894:897	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	4	73	theme	nanodimensional	695:709	arg1	network					711:717	nanodimensional network	695:717	nanodimensional network	695:717	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	6	74	theme	tensile	1000:1006	arg1	strength					1008:1015	the tensile strength	996:1015	the tensile strength	996:1015	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	4	75	theme	X-ray	526:530	arg1	diffraction					532:542	X-ray diffraction	526:542	X-ray diffraction (XRD)	526:548	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	4	75	theme	X-ray	526:530	arg1	XRD					545:547	XRD	545:547	XRD	545:547	The X-ray diffraction (XRD) and scanning electron microscopy (SEM) analysis revealed that the novel BC-HA/SS composites had a typical type I cellulose crystalline form with nanodimensional network, and microfibrils.					
32721822	5	76	theme	membranes	766:774	arg1	roughness					742:750	The roughness	738:750	The roughness of the hybrid membranes	738:774	The roughness of the hybrid membranes was analyzed by atomic force microscopy (AFM).					
32721822	3	77	dep	Fourier	398:404	arg1	transform					406:414	transform	406:414	transform infrared spectroscopy (FTIR)	406:443	Fourier transform infrared spectroscopy (FTIR) showed that the HA and SS were successfully integrated into the BC membrane.					
32721822	1	78	from	interest	184:191	arg1	field					224:228	the biomedical and cosmetic field	196:228	the biomedical and cosmetic field due to its unique structure and properties	196:271	Bacterial cellulose (BC) is a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties.					
32721822	5	79	theme	force	799:803	arg1	AFM					817:819	AFM	817:819	AFM	817:819	The roughness of the hybrid membranes was analyzed by atomic force microscopy (AFM).					
32721822	5	79	theme	force	799:803	arg1	microscopy					805:814	atomic force microscopy	792:814	atomic force microscopy (AFM)	792:820	The roughness of the hybrid membranes was analyzed by atomic force microscopy (AFM).					
32721822	6	80	theme	BC-HA/SS	976:983	arg1	composite					985:993	in situ modified BC-HA/SS composite	959:993	in situ modified BC-HA/SS composite	959:993	Thermogravimetric analysis showed that the thermal stability of the ex situ modified BC-HA/SS composite was better than the pure BC and in situ modified BC-HA/SS composite, the tensile strength and moisture content values of the ex situ modified BC-HA/SS were also the highest compared with that of the pure BC and in situ modified BC-HA/SS.					
32721822	0	81	theme	hyaluronic	70:79	arg1	acid					81:84	hyaluronic acid	70:84	hyaluronic acid	70:84	Production and characterization of bacterial cellulose membranes with hyaluronic acid and silk sericin.					
32721822	3	82	dep	transform	406:414	arg1	infrared					416:423	infrared	416:423	transform infrared spectroscopy (FTIR)	406:443	Fourier transform infrared spectroscopy (FTIR) showed that the HA and SS were successfully integrated into the BC membrane.					
32721822	1	83	theme	natural	134:140	arg1	polysaccharide					142:155	a natural polysaccharide	132:155	a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties	132:271	Bacterial cellulose (BC) is a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties.					
32721822	1	83	theme	natural	134:140	arg1	cellulose					114:122	Bacterial cellulose	104:122	Bacterial cellulose (BC)	104:127	Bacterial cellulose (BC) is a natural polysaccharide that has gained increasing interest in the biomedical and cosmetic field due to its unique structure and properties.					
33712146	0	0	theme	tea	77:79	arg1	"					80:80	"mountain tea"	67:80	"mountain tea"	67:80	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)					
33712146	6	1	theme	residues	857:864	arg1	blocks					817:822	large blocks	811:822	large blocks of highly methyl-esterified GalA residues	811:864	Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues.					
33712146	7	2	theme	residues	926:933	arg1	blocks					896:901	blocks	896:901	blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain	896:1004	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	8	3	theme	commercial	1123:1132	arg1	exploitation					1134:1145	commercial exploitation	1123:1145	commercial exploitation	1123:1145	The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.					
33712146	7	4	theme	HG	996:997	arg1	domain					999:1004	HG domain	996:1004	HG domain	996:1004	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	0	5	theme	mountain	68:75	arg1	"					80:80	"mountain tea"	67:80	"mountain tea"	67:80	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)					
33712146	6	6	theme	GalA	852:855	arg1	residues					857:864	highly methyl-esterified GalA residues	827:864	highly methyl-esterified GalA residues	827:864	Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues.					
33712146	5	7	theme	HG	659:660	arg1	enzymes					672:678	HG degrading enzymes	659:678	HG degrading enzymes	659:678	Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail.					
33712146	6	8	theme	methyl-esterified	834:850	arg1	residues					857:864	highly methyl-esterified GalA residues	827:864	highly methyl-esterified GalA residues	827:864	Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues.					
33712146	4	9	dep	weight	566:571	arg1	population					573:582	population	573:582	a high molecular weight population (∼60-100 kDa)	549:596	The pectin had a high molecular weight population (∼60-100 kDa).					
33712146	2	10	theme	uronic	299:304	arg1	%					321:321	72.4 mol%	313:321	72.4 mol%	313:321	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	10	theme	uronic	299:304	arg1	acids					306:310	uronic acids	299:310	uronic acids (72.4 mol%)	299:322	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	5	11	theme	degrading	662:670	arg1	enzymes					672:678	HG degrading enzymes	659:678	HG degrading enzymes	659:678	Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail.					
33712146	7	12	theme	GalA	979:982	arg1	residues					984:991	partly methyl-esterified and acetylated GalA residues	939:991	partly methyl-esterified and acetylated GalA residues	939:991	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	7	13	from	domain	999:1004	arg1	blocks					896:901	blocks	896:901	blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain	896:1004	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	5	14	theme	enzymes	672:678	arg1	combination					644:654	a combination	642:654	a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC	642:708	Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail.					
33712146	8	15	theme	mountain	1150:1157	arg1	tea					1159:1161	mountain tea	1150:1161	mountain tea	1150:1161	The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.					
33712146	7	16	theme	blocks	896:901	arg1	presence					884:891	the presence	880:891	the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain	880:1004	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	2	17	theme	glucose	424:430	arg1	galactose					398:406	galactose	398:406	galactose (14.5 mol%)	398:418	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	17	theme	glucose	424:430	arg1	%					440:440	6.2 mol%	433:440	6.2 mol%	433:440	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	17	theme	glucose	424:430	arg1	amounts					387:393	smaller amounts	379:393	smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%)	379:441	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	17	theme	glucose	424:430	arg1	%					417:417	14.5 mol%	409:417	14.5 mol%	409:417	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	17	theme	glucose	424:430	arg1	glucose					424:430	glucose	424:430	glucose (6.2 mol%)	424:441	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	5	18	from	structure	725:733	arg1	detail					746:751	greater detail	738:751	greater detail	738:751	Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail.					
33712146	0	19	theme	polysaccharide	29:42	arg1	fraction					44:51	a pectic polysaccharide fraction	20:51	a pectic polysaccharide fraction isolated from "mountain tea"	20:80	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)					
33712146	3	20	theme	methyl-esterified	483:499	arg1	homogalacturonan					501:516	a highly methyl-esterified homogalacturonan	474:516	a highly methyl-esterified homogalacturonan (HG)	474:521	The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) - 71 mol%.					
33712146	3	20	theme	methyl-esterified	483:499	arg1	HG					519:520	HG	519:520	HG	519:520	The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) - 71 mol%.					
33712146	2	21	theme	smaller	379:385	arg1	galactose					398:406	galactose	398:406	galactose (14.5 mol%)	398:418	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	21	theme	smaller	379:385	arg1	%					440:440	6.2 mol%	433:440	6.2 mol%	433:440	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	21	theme	smaller	379:385	arg1	amounts					387:393	smaller amounts	379:393	smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%)	379:441	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	21	theme	smaller	379:385	arg1	%					417:417	14.5 mol%	409:417	14.5 mol%	409:417	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	21	theme	smaller	379:385	arg1	glucose					424:430	glucose	424:430	glucose (6.2 mol%)	424:441	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	6	22	theme	large	811:815	arg1	blocks					817:822	large blocks	811:822	large blocks of highly methyl-esterified GalA residues	811:864	Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues.					
33712146	8	23	theme	biological	1099:1108	arg1	activity					1110:1117	biological activity	1099:1117	biological activity	1099:1117	The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.					
33712146	2	24	theme	mol	318:320	arg1	%					321:321	72.4 mol%	313:321	72.4 mol%	313:321	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	24	theme	mol	318:320	arg1	acids					306:310	uronic acids	299:310	uronic acids (72.4 mol%)	299:322	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	0	25	theme	Structural	0:9	arg1	Griseb					102:107	Griseb	102:107	Griseb	102:107	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)					
33712146	0	25	theme	Structural	0:9	arg1	study					11:15	Structural study	0:15	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)	0:109	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)					
33712146	1	26	theme	structural	163:172	arg1	characteristics					174:188	the structural characteristics	159:188	the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea	159:251	The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea.					
33712146	5	27	theme	greater	738:744	arg1	detail					746:751	greater detail	738:751	greater detail	738:751	Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail.					
33712146	0	28	attach	isolated	53:60	arg2	fraction					44:51	a pectic polysaccharide fraction	20:51	a pectic polysaccharide fraction isolated from "mountain tea"	20:80	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)					
33712146	0	28	attach	isolated	53:60	arg1	"					80:80	"mountain tea"	67:80	"mountain tea"	67:80	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)					
33712146	2	29	dep	predominated	337:348	arg1	followed					367:374	followed	367:374	followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%)	367:441	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	30	theme	mol	414:416	arg1	galactose					398:406	galactose	398:406	galactose (14.5 mol%)	398:418	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	30	theme	mol	414:416	arg1	%					417:417	14.5 mol%	409:417	14.5 mol%	409:417	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	7	31	theme	methyl-esterified	946:962	arg1	residues					984:991	partly methyl-esterified and acetylated GalA residues	939:991	partly methyl-esterified and acetylated GalA residues	939:991	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	4	32	theme	molecular	556:564	arg1	kDa					593:595	∼60-100 kDa	585:595	∼60-100 kDa	585:595	The pectin had a high molecular weight population (∼60-100 kDa).					
33712146	4	32	theme	molecular	556:564	arg1	weight					566:571	a high molecular weight population	549:582	a high molecular weight population (∼60-100 kDa)	549:596	The pectin had a high molecular weight population (∼60-100 kDa).					
33712146	2	33	theme	mol	437:439	arg1	%					440:440	6.2 mol%	433:440	6.2 mol%	433:440	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	33	theme	mol	437:439	arg1	glucose					424:430	glucose	424:430	glucose (6.2 mol%)	424:441	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	1	34	from	tea	249:251	arg1	fraction					226:233	an acid-extracted polysaccharide fraction	193:233	an acid-extracted polysaccharide fraction from mountain tea	193:251	The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea.					
33712146	1	34	from	tea	249:251	arg1	characteristics					174:188	the structural characteristics	159:188	the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea	159:251	The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea.					
33712146	8	35	theme	research	1028:1035	arg1	findings					1037:1044	The research findings	1024:1044	The research findings	1024:1044	The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.					
33712146	2	36	theme	galactose	398:406	arg1	galactose					398:406	galactose	398:406	galactose (14.5 mol%)	398:418	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	36	theme	galactose	398:406	arg1	%					440:440	6.2 mol%	433:440	6.2 mol%	433:440	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	36	theme	galactose	398:406	arg1	amounts					387:393	smaller amounts	379:393	smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%)	379:441	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	36	theme	galactose	398:406	arg1	%					417:417	14.5 mol%	409:417	14.5 mol%	409:417	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	2	36	theme	galactose	398:406	arg1	glucose					424:430	glucose	424:430	glucose (6.2 mol%)	424:441	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	0	37	theme	pectic	22:27	arg1	fraction					44:51	a pectic polysaccharide fraction	20:51	a pectic polysaccharide fraction isolated from "mountain tea"	20:80	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)					
33712146	7	38	theme	acetylated	968:977	arg1	residues					984:991	partly methyl-esterified and acetylated GalA residues	939:991	partly methyl-esterified and acetylated GalA residues	939:991	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	1	39	theme	acid-extracted	196:209	arg1	fraction					226:233	an acid-extracted polysaccharide fraction	193:233	an acid-extracted polysaccharide fraction from mountain tea	193:251	The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea.					
33712146	5	40	theme	Enzymatic	599:607	arg1	fingerprinting					609:622	Enzymatic fingerprinting	599:622	Enzymatic fingerprinting	599:622	Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail.					
33712146	3	41	theme	mol	528:530	arg1	%					531:531	71 mol%	525:531	71 mol%	525:531	The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) - 71 mol%.					
33712146	6	42	theme	blocks	817:822	arg1	presence					799:806	the presence	795:806	the presence of large blocks of highly methyl-esterified GalA residues	795:864	Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues.					
33712146	0	43	theme	fraction	44:51	arg1	Griseb					102:107	Griseb	102:107	Griseb	102:107	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)					
33712146	0	43	theme	fraction	44:51	arg1	study					11:15	Structural study	0:15	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)	0:109	Structural study of a pectic polysaccharide fraction isolated from "mountain tea" (Sideritis scardica Griseb.)					
33712146	1	44	from	characteristics	174:188	arg1	tea					249:251	mountain tea	240:251	mountain tea	240:251	The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea.					
33712146	7	45	theme	residues	984:991	arg1	blocks					896:901	blocks	896:901	blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain	896:1004	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	2	46	theme	monosaccharide	258:271	arg1	composition					273:283	The monosaccharide composition	254:283	The monosaccharide composition	254:283	The monosaccharide composition revealed that uronic acids (72.4 mol%) considerably predominated in the fraction, followed by smaller amounts of galactose (14.5 mol%) and glucose (6.2 mol%).					
33712146	7	47	theme	non-esterified	906:919	arg1	residues					926:933	non-esterified GalA residues	906:933	non-esterified GalA residues	906:933	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	7	48	from	residues	984:991	arg1	domain					999:1004	HG domain	996:1004	HG domain	996:1004	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	1	49	theme	polysaccharide	211:224	arg1	fraction					226:233	an acid-extracted polysaccharide fraction	193:233	an acid-extracted polysaccharide fraction from mountain tea	193:251	The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea.					
33712146	8	50	theme	further	1066:1072	arg1	investigations					1074:1087	further investigations	1066:1087	further investigations regarding biological activity and commercial exploitation of mountain tea	1066:1161	The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.					
33712146	6	51	theme	Unsaturated	754:764	arg1	oligomers					766:774	Unsaturated oligomers	754:774	Unsaturated oligomers released	754:783	Unsaturated oligomers released indicated the presence of large blocks of highly methyl-esterified GalA residues.					
33712146	8	52	theme	tea	1159:1161	arg1	activity					1110:1117	biological activity	1099:1117	biological activity	1099:1117	The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.					
33712146	8	52	theme	tea	1159:1161	arg1	exploitation					1134:1145	commercial exploitation	1123:1145	commercial exploitation	1123:1145	The research findings provide a basis for further investigations regarding biological activity and commercial exploitation of mountain tea.					
33712146	1	53	theme	fraction	226:233	arg1	characteristics					174:188	the structural characteristics	159:188	the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea	159:251	The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea.					
33712146	5	54	theme	LC-HILIC-MS	684:694	arg1	combination					644:654	a combination	642:654	a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC	642:708	Enzymatic fingerprinting was employed with a combination of HG degrading enzymes and LC-HILIC-MS, HPAEC, HPSEC to examine the structure in greater detail.					
33712146	1	55	theme	present	116:122	arg1	study					124:128	The present study	112:128	The present study	112:128	The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea.					
33712146	7	56	from	residues	926:933	arg1	domain					999:1004	HG domain	996:1004	HG domain	996:1004	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	4	57	theme	high	551:554	arg1	kDa					593:595	∼60-100 kDa	585:595	∼60-100 kDa	585:595	The pectin had a high molecular weight population (∼60-100 kDa).					
33712146	4	57	theme	high	551:554	arg1	weight					566:571	a high molecular weight population	549:582	a high molecular weight population (∼60-100 kDa)	549:596	The pectin had a high molecular weight population (∼60-100 kDa).					
33712146	3	58	contain	contained	457:465	arg2	HG					519:520	HG	519:520	HG	519:520	The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) - 71 mol%.					
33712146	3	58	contain	contained	457:465	arg2	%					531:531	71 mol%	525:531	71 mol%	525:531	The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) - 71 mol%.					
33712146	3	58	contain	contained	457:465	arg1	fraction					448:455	The fraction	444:455	The fraction	444:455	The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) - 71 mol%.					
33712146	3	58	contain	contained	457:465	arg2	homogalacturonan					501:516	a highly methyl-esterified homogalacturonan	474:516	a highly methyl-esterified homogalacturonan (HG)	474:521	The fraction contained mostly a highly methyl-esterified homogalacturonan (HG) - 71 mol%.					
33712146	4	59	contain	had	545:547	arg2	kDa					593:595	∼60-100 kDa	585:595	∼60-100 kDa	585:595	The pectin had a high molecular weight population (∼60-100 kDa).					
33712146	4	59	contain	had	545:547	arg2	weight					566:571	a high molecular weight population	549:582	a high molecular weight population (∼60-100 kDa)	549:596	The pectin had a high molecular weight population (∼60-100 kDa).					
33712146	4	59	contain	had	545:547	arg1	pectin					538:543	The pectin	534:543	The pectin	534:543	The pectin had a high molecular weight population (∼60-100 kDa).					
33712146	7	60	theme	GalA	921:924	arg1	residues					926:933	non-esterified GalA residues	906:933	non-esterified GalA residues	906:933	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	7	61	from	blocks	896:901	arg1	domain					999:1004	HG domain	996:1004	HG domain	996:1004	Furthermore, the presence of blocks of non-esterified GalA residues and partly methyl-esterified and acetylated GalA residues in HG domain was demonstrated.					
33712146	1	62	theme	mountain	240:247	arg1	tea					249:251	mountain tea	240:251	mountain tea	240:251	The present study was conducted to investigate the structural characteristics of an acid-extracted polysaccharide fraction from mountain tea.					
34657564	4	0	theme	hot	548:550	arg1	method					558:563	the hot water method	544:563	the hot water method	544:563	Additionally, the immunoregulatory activities of E2P and another polysaccharide (ESBP, extracted using the hot water method) were compared.					
34657564	7	1	theme	E2P	942:944	arg1	starch					910:915	starch	910:915	starch	910:915	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	7	1	theme	E2P	942:944	arg1	sugar					890:894	The total sugar	880:894	The total sugar	880:894	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	7	1	theme	E2P	942:944	arg1	acid					904:907	uronic acid	897:907	uronic acid	897:907	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	7	1	theme	E2P	942:944	arg1	%					956:956	81.77% ±	951:958	81.77% ± 2.84%	951:964	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	7	1	theme	E2P	942:944	arg1	contents					930:937	protein contents	922:937	protein contents of E2P	922:944	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	9	2	from	tests	1192:1196	arg1	cells					1210:1214	RAW264.7 cells	1201:1214	RAW264.7 cells	1201:1214	The in vitro tests in RAW264.7 cells indicated that ESBP exhibited better immunomodulatory activities than E2P.					
34657564	1	3	theme	response	277:284	arg1	methodology					294:304	response surface methodology	277:304	response surface methodology	277:304	An efficient compound enzyme extraction process was developed and optimized to extract the polysaccharide from Erythronium sibiricum bulb via response surface methodology.					
34657564	0	4	theme	sibiricum	83:91	arg1	bulb					93:96	Erythronium sibiricum bulb	71:96	Erythronium sibiricum bulb	71:96	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	1	5	theme	surface	286:292	arg1	methodology					294:304	response surface methodology	277:304	response surface methodology	277:304	An efficient compound enzyme extraction process was developed and optimized to extract the polysaccharide from Erythronium sibiricum bulb via response surface methodology.					
34657564	5	6	theme	%	716:716	arg1	ratio					706:710	enzyme concentration ratio	685:710	enzyme concentration ratio (0.5% cellulase:1.5% amylase)	685:740	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	6	theme	%	716:716	arg1	amylase					733:739	0.5% cellulase:1.5% amylase	713:739	0.5% cellulase:1.5% amylase	713:739	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	11	7	theme	phagocytosis	1527:1538	arg1	ability					1540:1546	phagocytosis ability	1527:1546	phagocytosis ability	1527:1546	By contrast, E2P can only promote phagocytosis ability and the secretion of IL-1β.					
34657564	9	8	theme	RAW264.7	1201:1208	arg1	cells					1210:1214	RAW264.7 cells	1201:1214	RAW264.7 cells	1201:1214	The in vitro tests in RAW264.7 cells indicated that ESBP exhibited better immunomodulatory activities than E2P.					
34657564	4	9	dep	polysaccharide	506:519	arg1	ESBP					522:525	ESBP	522:525	ESBP	522:525	Additionally, the immunoregulatory activities of E2P and another polysaccharide (ESBP, extracted using the hot water method) were compared.					
34657564	5	10	theme	cellulase:1.5	718:730	arg1	ratio					706:710	enzyme concentration ratio	685:710	enzyme concentration ratio (0.5% cellulase:1.5% amylase)	685:740	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	10	theme	cellulase:1.5	718:730	arg1	amylase					733:739	0.5% cellulase:1.5% amylase	713:739	0.5% cellulase:1.5% amylase	713:739	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	6	11	theme	ESBP	857:860	arg1	yield					848:852	the yield	844:852	the yield of ESBP	844:860	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions was considerably higher than the yield of ESBP (37.25% ± 0.17%).					
34657564	4	12	theme	immunoregulatory	459:474	arg1	activities					476:485	the immunoregulatory activities	455:485	the immunoregulatory activities of E2P and another polysaccharide (ESBP, extracted using the hot water method)	455:564	Additionally, the immunoregulatory activities of E2P and another polysaccharide (ESBP, extracted using the hot water method) were compared.					
34657564	3	13	theme	E2P	385:387	arg1	characteristics					366:380	the preliminary characteristics	350:380	the preliminary characteristics of E2P	350:387	Then, the preliminary characteristics of E2P were determined via colorimetry and chromatography.					
34657564	7	14	theme	%	971:971	arg1	%					979:979	3.31% ± 0.45%	967:979	3.31% ± 0.45%	967:979	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	7	15	theme	total	884:888	arg1	sugar					890:894	The total sugar	880:894	The total sugar	880:894	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	7	15	theme	total	884:888	arg1	%					956:956	81.77% ±	951:958	81.77% ± 2.84%	951:964	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	2	16	theme	polysaccharide	311:324	arg1	E2P					326:328	The polysaccharide E2P	307:328	The polysaccharide E2P	307:328	The polysaccharide E2P was obtained.					
34657564	5	17	dep	were	614:617	arg1	ratio					706:710	enzyme concentration ratio	685:710	enzyme concentration ratio (0.5% cellulase:1.5% amylase)	685:740	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	17	dep	were	614:617	arg1	time					655:658	time	655:658	time (2.52 h)	655:667	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	17	dep	were	614:617	arg1	temperature					631:641	temperature	631:641	temperature (54.56 °C)	631:652	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	17	dep	were	614:617	arg1	2.52 h					661:666	2.52 h	661:666	2.52 h	661:666	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	17	dep	were	614:617	arg1	54.56 °C					644:651	54.56 °C	644:651	54.56 °C	644:651	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	17	dep	were	614:617	arg1	pH					670:671	pH (6.53)	670:678	pH (6.53)	670:678	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	17	dep	were	614:617	arg1	amylase					733:739	0.5% cellulase:1.5% amylase	713:739	0.5% cellulase:1.5% amylase	713:739	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	0	18	theme	immunoregulatory	106:121	arg1	activities					123:132	its immunoregulatory activities	102:132	its immunoregulatory activities	102:132	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	0	19	from	extraction	37:46	arg1	bulb					93:96	Erythronium sibiricum bulb	71:96	Erythronium sibiricum bulb	71:96	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	0	19	from	extraction	37:46	arg1	activities					123:132	its immunoregulatory activities	102:132	its immunoregulatory activities	102:132	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	7	20	theme	±	973:973	arg1	%					979:979	3.31% ± 0.45%	967:979	3.31% ± 0.45%	967:979	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	6	21	theme	±	761:761	arg1	%					767:767	64.18% ± 2.91%	754:767	64.18% ± 2.91%	754:767	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions was considerably higher than the yield of ESBP (37.25% ± 0.17%).					
34657564	6	21	theme	±	761:761	arg1	yield					747:751	The yield	743:751	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions	743:813	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions was considerably higher than the yield of ESBP (37.25% ± 0.17%).					
34657564	8	22	theme	molar	1151:1155	arg1	ratio					1157:1161	a molar ratio	1149:1161	a molar ratio of 543.2:1:1.8	1149:1176	The HPLC result suggested that the predominant monosaccharides of E2P included glucose, galactose, and arabinose, with a molar ratio of 543.2:1:1.8.					
34657564	5	23	theme	%	731:731	arg1	ratio					706:710	enzyme concentration ratio	685:710	enzyme concentration ratio (0.5% cellulase:1.5% amylase)	685:740	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	23	theme	%	731:731	arg1	amylase					733:739	0.5% cellulase:1.5% amylase	713:739	0.5% cellulase:1.5% amylase	713:739	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	7	24	theme	0.24	1001:1004	arg1	%					1013:1013	0.24% ± 0.02%	1001:1013	0.24% ± 0.02%	1001:1013	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	3	25	theme	preliminary	354:364	arg1	characteristics					366:380	the preliminary characteristics	350:380	the preliminary characteristics of E2P	350:387	Then, the preliminary characteristics of E2P were determined via colorimetry and chromatography.					
34657564	6	26	theme	%	759:759	arg1	%					767:767	64.18% ± 2.91%	754:767	64.18% ± 2.91%	754:767	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions was considerably higher than the yield of ESBP (37.25% ± 0.17%).					
34657564	6	26	theme	%	759:759	arg1	yield					747:751	The yield	743:751	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions	743:813	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions was considerably higher than the yield of ESBP (37.25% ± 0.17%).					
34657564	8	27	theme	543.2:1:1.8	1166:1176	arg1	ratio					1157:1161	a molar ratio	1149:1161	a molar ratio of 543.2:1:1.8	1149:1176	The HPLC result suggested that the predominant monosaccharides of E2P included glucose, galactose, and arabinose, with a molar ratio of 543.2:1:1.8.					
34657564	9	28	theme	better	1246:1251	arg1	activities					1270:1279	better immunomodulatory activities	1246:1279	better immunomodulatory activities	1246:1279	The in vitro tests in RAW264.7 cells indicated that ESBP exhibited better immunomodulatory activities than E2P.					
34657564	7	29	theme	%	1005:1005	arg1	%					1013:1013	0.24% ± 0.02%	1001:1013	0.24% ± 0.02%	1001:1013	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	10	30	theme	nitric	1411:1416	arg1	oxide					1418:1422	nitric oxide	1411:1422	nitric oxide	1411:1422	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	9	31	theme	immunomodulatory	1253:1268	arg1	activities					1270:1279	better immunomodulatory activities	1246:1279	better immunomodulatory activities	1246:1279	The in vitro tests in RAW264.7 cells indicated that ESBP exhibited better immunomodulatory activities than E2P.					
34657564	0	32	theme	composite	20:28	arg1	extraction					37:46	the composite enzyme extraction	16:46	the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities	16:132	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	7	33	dep	%	956:956	arg1	%					964:964	2.84%	960:964	81.77% ± 2.84%	951:964	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	5	34	theme	concentration	692:704	arg1	ratio					706:710	enzyme concentration ratio	685:710	enzyme concentration ratio (0.5% cellulase:1.5% amylase)	685:740	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	34	theme	concentration	692:704	arg1	amylase					733:739	0.5% cellulase:1.5% amylase	713:739	0.5% cellulase:1.5% amylase	713:739	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	10	35	theme	interleukin	1454:1464	arg1	-1β					1470:1472	interleukin (IL)-1β	1454:1472	interleukin (IL)-1β of RAW264.6 cells	1454:1490	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	10	36	theme	cytokines	1392:1400	arg1	abilities					1379:1387	cytokine secretion abilities	1360:1387	cytokine secretion abilities	1360:1387	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	10	36	theme	cytokines	1392:1400	arg1	phagocytosis					1342:1353	phagocytosis	1342:1353	phagocytosis	1342:1353	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	10	36	theme	cytokines	1392:1400	arg1	proliferation					1327:1339	the proliferation	1323:1339	the proliferation	1323:1339	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	0	37	from	bulb	93:96	arg1	extraction					37:46	the composite enzyme extraction	16:46	the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities	16:132	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	0	37	from	bulb	93:96	arg1	polysaccharide					51:64	polysaccharide	51:64	polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities	51:132	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	6	38	theme	%	868:868	arg1	%					876:876	37.25% ± 0.17%	863:876	37.25% ± 0.17%	863:876	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions was considerably higher than the yield of ESBP (37.25% ± 0.17%).					
34657564	8	39	theme	predominant	1065:1075	arg1	monosaccharides					1077:1091	the predominant monosaccharides	1061:1091	the predominant monosaccharides of E2P	1061:1098	The HPLC result suggested that the predominant monosaccharides of E2P included glucose, galactose, and arabinose, with a molar ratio of 543.2:1:1.8.					
34657564	0	40	theme	extraction	37:46	arg1	Optimization					0:11	Optimization	0:11	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.	0:133	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	7	41	theme	±	1007:1007	arg1	%					1013:1013	0.24% ± 0.02%	1001:1013	0.24% ± 0.02%	1001:1013	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	8	42	theme	E2P	1096:1098	arg1	monosaccharides					1077:1091	the predominant monosaccharides	1061:1091	the predominant monosaccharides of E2P	1061:1098	The HPLC result suggested that the predominant monosaccharides of E2P included glucose, galactose, and arabinose, with a molar ratio of 543.2:1:1.8.					
34657564	10	43	theme	necrosis	1431:1438	arg1	factor-α					1440:1447	tumor necrosis factor-α	1425:1447	tumor necrosis factor-α	1425:1447	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	4	44	theme	polysaccharide	506:519	arg1	activities					476:485	the immunoregulatory activities	455:485	the immunoregulatory activities of E2P and another polysaccharide (ESBP, extracted using the hot water method)	455:564	Additionally, the immunoregulatory activities of E2P and another polysaccharide (ESBP, extracted using the hot water method) were compared.					
34657564	0	45	theme	enzyme	30:35	arg1	extraction					37:46	the composite enzyme extraction	16:46	the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities	16:132	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	9	46	dep	in	1183:1184	arg1	vitro					1186:1190	vitro	1186:1190	vitro	1186:1190	The in vitro tests in RAW264.7 cells indicated that ESBP exhibited better immunomodulatory activities than E2P.					
34657564	5	47	theme	optimized	585:593	arg1	results					606:612	The optimized extraction results	581:612	The optimized extraction results	581:612	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	10	48	theme	cytokine	1360:1367	arg1	abilities					1379:1387	cytokine secretion abilities	1360:1387	cytokine secretion abilities	1360:1387	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	0	49	theme	polysaccharide	51:64	arg1	extraction					37:46	the composite enzyme extraction	16:46	the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities	16:132	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	1	50	from	bulb	268:271	arg1	polysaccharide					226:239	the polysaccharide	222:239	the polysaccharide from Erythronium sibiricum bulb	222:271	An efficient compound enzyme extraction process was developed and optimized to extract the polysaccharide from Erythronium sibiricum bulb via response surface methodology.					
34657564	5	51	theme	extraction	595:604	arg1	results					606:612	The optimized extraction results	581:612	The optimized extraction results	581:612	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	10	52	theme	cells	1486:1490	arg1	oxide					1418:1422	nitric oxide	1411:1422	nitric oxide	1411:1422	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	10	52	theme	cells	1486:1490	arg1	-1β					1470:1472	interleukin (IL)-1β	1454:1472	interleukin (IL)-1β of RAW264.6 cells	1454:1490	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	10	52	theme	cells	1486:1490	arg1	factor-α					1440:1447	tumor necrosis factor-α	1425:1447	tumor necrosis factor-α	1425:1447	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	7	53	theme	±	988:988	arg1	%					994:994	3.29% ± 0.01%	982:994	3.29% ± 0.01%	982:994	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	6	54	theme	±	870:870	arg1	%					876:876	37.25% ± 0.17%	863:876	37.25% ± 0.17%	863:876	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions was considerably higher than the yield of ESBP (37.25% ± 0.17%).					
34657564	0	55	from	activities	123:132	arg1	extraction					37:46	the composite enzyme extraction	16:46	the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities	16:132	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	0	55	from	activities	123:132	arg1	polysaccharide					51:64	polysaccharide	51:64	polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities	51:132	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	6	56	dep	higher	832:837	arg1	%					876:876	37.25% ± 0.17%	863:876	37.25% ± 0.17%	863:876	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions was considerably higher than the yield of ESBP (37.25% ± 0.17%).					
34657564	10	57	theme	tumor	1425:1429	arg1	factor-α					1440:1447	tumor necrosis factor-α	1425:1447	tumor necrosis factor-α	1425:1447	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	6	58	theme	aforementioned	789:802	arg1	conditions					804:813	the aforementioned conditions	785:813	the aforementioned conditions	785:813	The yield (64.18% ± 2.91%) obtained under the aforementioned conditions was considerably higher than the yield of ESBP (37.25% ± 0.17%).					
34657564	11	59	theme	IL-1β	1569:1573	arg1	ability					1540:1546	phagocytosis ability	1527:1546	phagocytosis ability	1527:1546	By contrast, E2P can only promote phagocytosis ability and the secretion of IL-1β.					
34657564	11	59	theme	IL-1β	1569:1573	arg1	secretion					1556:1564	the secretion	1552:1564	the secretion of IL-1β	1552:1573	By contrast, E2P can only promote phagocytosis ability and the secretion of IL-1β.					
34657564	1	60	theme	efficient	138:146	arg1	process					175:181	An efficient compound enzyme extraction process	135:181	An efficient compound enzyme extraction process	135:181	An efficient compound enzyme extraction process was developed and optimized to extract the polysaccharide from Erythronium sibiricum bulb via response surface methodology.					
34657564	9	61	theme	in	1183:1184	arg1	tests					1192:1196	The in vitro tests	1179:1196	The in vitro tests in RAW264.7 cells	1179:1214	The in vitro tests in RAW264.7 cells indicated that ESBP exhibited better immunomodulatory activities than E2P.					
34657564	1	62	theme	Erythronium	246:256	arg1	bulb					268:271	Erythronium sibiricum bulb	246:271	Erythronium sibiricum bulb	246:271	An efficient compound enzyme extraction process was developed and optimized to extract the polysaccharide from Erythronium sibiricum bulb via response surface methodology.					
34657564	5	63	theme	enzyme	685:690	arg1	ratio					706:710	enzyme concentration ratio	685:710	enzyme concentration ratio (0.5% cellulase:1.5% amylase)	685:740	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	5	63	theme	enzyme	685:690	arg1	amylase					733:739	0.5% cellulase:1.5% amylase	713:739	0.5% cellulase:1.5% amylase	713:739	The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase).					
34657564	7	64	theme	protein	922:928	arg1	contents					930:937	protein contents	922:937	protein contents of E2P	922:944	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	1	65	theme	compound	148:155	arg1	process					175:181	An efficient compound enzyme extraction process	135:181	An efficient compound enzyme extraction process	135:181	An efficient compound enzyme extraction process was developed and optimized to extract the polysaccharide from Erythronium sibiricum bulb via response surface methodology.					
34657564	8	66	theme	HPLC	1034:1037	arg1	result					1039:1044	The HPLC result	1030:1044	The HPLC result	1030:1044	The HPLC result suggested that the predominant monosaccharides of E2P included glucose, galactose, and arabinose, with a molar ratio of 543.2:1:1.8.					
34657564	1	67	theme	sibiricum	258:266	arg1	bulb					268:271	Erythronium sibiricum bulb	246:271	Erythronium sibiricum bulb	246:271	An efficient compound enzyme extraction process was developed and optimized to extract the polysaccharide from Erythronium sibiricum bulb via response surface methodology.					
34657564	0	68	theme	Erythronium	71:81	arg1	bulb					93:96	Erythronium sibiricum bulb	71:96	Erythronium sibiricum bulb	71:96	Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities.					
34657564	4	69	theme	E2P	490:492	arg1	activities					476:485	the immunoregulatory activities	455:485	the immunoregulatory activities of E2P and another polysaccharide (ESBP, extracted using the hot water method)	455:564	Additionally, the immunoregulatory activities of E2P and another polysaccharide (ESBP, extracted using the hot water method) were compared.					
34657564	1	70	theme	enzyme	157:162	arg1	process					175:181	An efficient compound enzyme extraction process	135:181	An efficient compound enzyme extraction process	135:181	An efficient compound enzyme extraction process was developed and optimized to extract the polysaccharide from Erythronium sibiricum bulb via response surface methodology.					
34657564	7	71	theme	%	986:986	arg1	%					994:994	3.29% ± 0.01%	982:994	3.29% ± 0.01%	982:994	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	4	72	theme	water	552:556	arg1	method					558:563	the hot water method	544:563	the hot water method	544:563	Additionally, the immunoregulatory activities of E2P and another polysaccharide (ESBP, extracted using the hot water method) were compared.					
34657564	10	73	theme	secretion	1369:1377	arg1	abilities					1379:1387	cytokine secretion abilities	1360:1387	cytokine secretion abilities	1360:1387	In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells.					
34657564	7	74	theme	uronic	897:902	arg1	acid					904:907	uronic acid	897:907	uronic acid	897:907	The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively.					
34657564	1	75	theme	extraction	164:173	arg1	process					175:181	An efficient compound enzyme extraction process	135:181	An efficient compound enzyme extraction process	135:181	An efficient compound enzyme extraction process was developed and optimized to extract the polysaccharide from Erythronium sibiricum bulb via response surface methodology.					
32270793	5	0	theme	macrophages	801:811	arg1	composition					786:796	the glycosphingolipids composition	763:796	the glycosphingolipids composition of macrophages	763:811	In contrast, infection of macrophages by M. bovis BCG did not modify the glycosphingolipids composition of macrophages.					
32270793	6	1	theme	Galβ1-4[Fucα1-3	972:986	arg1	epitope					996:1002	the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope	963:1002	the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope	963:1002	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	6	2	theme	embryonic	1034:1042	arg1	antigen-1					1044:1052	stage-specific embryonic antigen-1	1019:1052	stage-specific embryonic antigen-1	1019:1052	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	8	3	with	Activation	1213:1222	arg1	Pam3Lp19					1259:1266	the mycobacterial lipopeptide Pam3Lp19	1229:1266	the mycobacterial lipopeptide Pam3Lp19	1229:1266	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	8	3	with	Activation	1213:1222	arg1	agonist					1272:1278	an agonist	1269:1278	an agonist of toll-like receptor 2	1269:1302	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	4	4	theme	mycobacterial	582:594	arg1	infection					596:604	mycobacterial infection	582:604	mycobacterial infection	582:604	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32270793	6	5	theme	Lex	967:969	arg1	epitope					996:1002	the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope	963:1002	the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope	963:1002	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	7	6	theme	FACS	1154:1157	arg1	analysis					1159:1166	FACS analysis	1154:1166	FACS analysis using an α1,3-linked fucose specific lectin	1154:1210	Modification of the surface expression of Lex was further confirmed in both MDM and THP-1 cells by FACS analysis using an α1,3-linked fucose specific lectin.					
32270793	6	7	theme	N-glycans	870:878	arg1	analysis					852:859	Further nano-LC-MSn glycotope-centric analysis	814:859	Further nano-LC-MSn glycotope-centric analysis of total N-glycans	814:878	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	2	8	theme	lectins	291:297	arg1	binding					280:286	the binding	276:286	the binding of lectins involved in cell activation and adhesion	276:338	Indeed, the alteration of the cell-surface glycosylation status may affect the binding of lectins involved in cell activation and adhesion.					
32270793	4	9	theme	increased	614:622	arg1	synthesis					624:632	increased synthesis	614:632	increased synthesis of biantennary and multifucosylated complex type N-glycans	614:691	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32270793	6	10	theme	total	864:868	arg1	N-glycans					870:878	total N-glycans	864:878	total N-glycans	864:878	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	1	11	theme	Macrophage	67:76	arg1	glycosylation					78:90	Macrophage glycosylation	67:90	Macrophage glycosylation	67:90	Macrophage glycosylation is essential to initiate the host-immune defense but may also be targeted by pathogens to promote infection.					
32270793	8	12	theme	lipopeptide	1247:1257	arg1	Pam3Lp19					1259:1266	the mycobacterial lipopeptide Pam3Lp19	1229:1266	the mycobacterial lipopeptide Pam3Lp19	1229:1266	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	8	12	theme	lipopeptide	1247:1257	arg1	agonist					1272:1278	an agonist	1269:1278	an agonist of toll-like receptor 2	1269:1302	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	3	13	theme	macrophages	481:491	arg1	N-glycomes					425:434	the N-glycomes	421:434	the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1	421:527	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	4	14	gly	multifucosylated	653:668	arg1	N-glycans					683:691	biantennary and multifucosylated complex type N-glycans	637:691	biantennary and multifucosylated complex type N-glycans	637:691	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32270793	4	15	theme	multifucosylated	653:668	arg1	N-glycans					683:691	biantennary and multifucosylated complex type N-glycans	637:691	biantennary and multifucosylated complex type N-glycans	637:691	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32270793	3	16	theme	THP1	524:527	arg1	N-glycomes					425:434	the N-glycomes	421:434	the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1	421:527	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	9	17	theme	cell-surface	1516:1527	arg1	remodeling					1529:1538	infection-triggered cell-surface remodeling	1496:1538	infection-triggered cell-surface remodeling of macrophages	1496:1553	These results pave the way toward the understanding of infection-triggered cell-surface remodeling of macrophages.					
32270793	3	18	theme	N-glycomes	425:434	arg1	remodeling					407:416	the remodeling	403:416	the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1	403:527	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	5	19	theme	glycosphingolipids	767:784	arg1	composition					786:796	the glycosphingolipids composition	763:796	the glycosphingolipids composition of macrophages	763:811	In contrast, infection of macrophages by M. bovis BCG did not modify the glycosphingolipids composition of macrophages.					
32270793	0	20	theme	Mycobacterium	0:12	arg1	BCG					20:22	Mycobacterium bovis BCG	0:22	Mycobacterium bovis BCG infection	0:32	Mycobacterium bovis BCG infection alters the macrophage N-glycome.					
32270793	8	21	theme	receptor	1293:1300	arg1	Pam3Lp19					1259:1266	the mycobacterial lipopeptide Pam3Lp19	1229:1266	the mycobacterial lipopeptide Pam3Lp19	1229:1266	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	8	21	theme	receptor	1293:1300	arg1	agonist					1272:1278	an agonist	1269:1278	an agonist of toll-like receptor 2	1269:1302	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	0	22	theme	bovis	14:18	arg1	BCG					20:22	Mycobacterium bovis BCG	0:22	Mycobacterium bovis BCG infection	0:32	Mycobacterium bovis BCG infection alters the macrophage N-glycome.					
32270793	6	23	theme	increased	939:947	arg1	expression					949:958	an increased expression	936:958	an increased expression	936:958	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	6	24	theme	glycotope-centric	834:850	arg1	analysis					852:859	Further nano-LC-MSn glycotope-centric analysis	814:859	Further nano-LC-MSn glycotope-centric analysis of total N-glycans	814:878	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	8	25	theme	surface	1418:1424	arg1	glycosylation					1426:1438	surface glycosylation	1418:1438	surface glycosylation	1418:1438	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	9	26	theme	macrophages	1543:1553	arg1	remodeling					1529:1538	infection-triggered cell-surface remodeling	1496:1538	infection-triggered cell-surface remodeling of macrophages	1496:1553	These results pave the way toward the understanding of infection-triggered cell-surface remodeling of macrophages.					
32270793	3	27	theme	bovis	385:389	arg1	BCG					391:393	M. bovis BCG	382:393	M. bovis BCG	382:393	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	6	28	theme	stage-specific	1019:1032	arg1	antigen-1					1044:1052	stage-specific embryonic antigen-1	1019:1052	stage-specific embryonic antigen-1	1019:1052	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	6	29	theme	GlcNAc	988:993	arg1	epitope					996:1002	the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope	963:1002	the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope	963:1002	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	6	30	theme	nano-LC-MSn	822:832	arg1	analysis					852:859	Further nano-LC-MSn glycotope-centric analysis	814:859	Further nano-LC-MSn glycotope-centric analysis of total N-glycans	814:878	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	9	31	theme	remodeling	1529:1538	arg1	understanding					1479:1491	the understanding	1475:1491	the understanding of infection-triggered cell-surface remodeling of macrophages	1475:1553	These results pave the way toward the understanding of infection-triggered cell-surface remodeling of macrophages.					
32270793	4	32	theme	N-glycomic	545:554	arg1	analysis					556:563	MALDI-MS based N-glycomic analysis	530:563	MALDI-MS based N-glycomic analysis	530:563	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32270793	5	33	theme	M.	735:736	arg1	BCG					744:746	M. bovis BCG	735:746	M. bovis BCG	735:746	In contrast, infection of macrophages by M. bovis BCG did not modify the glycosphingolipids composition of macrophages.					
32270793	6	34	theme	Further	814:820	arg1	analysis					852:859	Further nano-LC-MSn glycotope-centric analysis	814:859	Further nano-LC-MSn glycotope-centric analysis of total N-glycans	814:878	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	7	35	link	α1,3-linked	1177:1187	arg1	lectin					1205:1210	an α1,3-linked fucose specific lectin	1174:1210	an α1,3-linked fucose specific lectin	1174:1210	Modification of the surface expression of Lex was further confirmed in both MDM and THP-1 cells by FACS analysis using an α1,3-linked fucose specific lectin.					
32270793	3	36	theme	primary	450:456	arg1	MDM					494:496	MDM	494:496	MDM	494:496	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	3	36	theme	primary	450:456	arg1	macrophages					481:491	human primary blood monocyte-derived macrophages	444:491	human primary blood monocyte-derived macrophages (MDM)	444:497	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	0	37	theme	BCG	20:22	arg1	infection					24:32	Mycobacterium bovis BCG infection	0:32	Mycobacterium bovis BCG infection	0:32	Mycobacterium bovis BCG infection alters the macrophage N-glycome.					
32270793	5	38	theme	bovis	738:742	arg1	BCG					744:746	M. bovis BCG	735:746	M. bovis BCG	735:746	In contrast, infection of macrophages by M. bovis BCG did not modify the glycosphingolipids composition of macrophages.					
32270793	3	39	theme	blood	458:462	arg1	MDM					494:496	MDM	494:496	MDM	494:496	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	3	39	theme	blood	458:462	arg1	macrophages					481:491	human primary blood monocyte-derived macrophages	444:491	human primary blood monocyte-derived macrophages (MDM)	444:497	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	7	40	theme	specific	1196:1203	arg1	lectin					1205:1210	an α1,3-linked fucose specific lectin	1174:1210	an α1,3-linked fucose specific lectin	1174:1210	Modification of the surface expression of Lex was further confirmed in both MDM and THP-1 cells by FACS analysis using an α1,3-linked fucose specific lectin.					
32270793	8	41	theme	overall	1324:1330	arg1	pattern					1345:1351	the overall fucosylation pattern	1320:1351	the overall fucosylation pattern	1320:1351	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	2	42	theme	cell-surface	231:242	arg1	status					258:263	the cell-surface glycosylation status	227:263	the cell-surface glycosylation status	227:263	Indeed, the alteration of the cell-surface glycosylation status may affect the binding of lectins involved in cell activation and adhesion.					
32270793	4	43	theme	N-glycans	683:691	arg1	synthesis					624:632	increased synthesis	614:632	increased synthesis of biantennary and multifucosylated complex type N-glycans	614:691	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32270793	7	44	theme	surface	1075:1081	arg1	expression					1083:1092	the surface expression	1071:1092	the surface expression of Lex	1071:1099	Modification of the surface expression of Lex was further confirmed in both MDM and THP-1 cells by FACS analysis using an α1,3-linked fucose specific lectin.					
32270793	3	45	theme	human	444:448	arg1	MDM					494:496	MDM	494:496	MDM	494:496	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	3	45	theme	human	444:448	arg1	macrophages					481:491	human primary blood monocyte-derived macrophages	444:491	human primary blood monocyte-derived macrophages (MDM)	444:497	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	7	46	theme	α1,3-linked	1177:1187	arg1	lectin					1205:1210	an α1,3-linked fucose specific lectin	1174:1210	an α1,3-linked fucose specific lectin	1174:1210	Modification of the surface expression of Lex was further confirmed in both MDM and THP-1 cells by FACS analysis using an α1,3-linked fucose specific lectin.					
32270793	6	47	theme	epitope	996:1002	arg1	expression					949:958	an increased expression	936:958	an increased expression	936:958	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	3	48	link	monocyte-derived	464:479	arg1	MDM					494:496	MDM	494:496	MDM	494:496	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	3	48	link	monocyte-derived	464:479	arg1	macrophages					481:491	human primary blood monocyte-derived macrophages	444:491	human primary blood monocyte-derived macrophages (MDM)	444:497	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	7	49	theme	expression	1083:1092	arg1	Modification					1055:1066	Modification	1055:1066	Modification of the surface expression of Lex	1055:1099	Modification of the surface expression of Lex was further confirmed in both MDM and THP-1 cells by FACS analysis using an α1,3-linked fucose specific lectin.					
32270793	1	50	theme	host-immune	121:131	arg1	defense					133:139	the host-immune defense	117:139	the host-immune defense	117:139	Macrophage glycosylation is essential to initiate the host-immune defense but may also be targeted by pathogens to promote infection.					
32270793	0	51	theme	macrophage	45:54	arg1	N-glycome					56:64	the macrophage N-glycome	41:64	the macrophage N-glycome	41:64	Mycobacterium bovis BCG infection alters the macrophage N-glycome.					
32270793	4	52	theme	based	539:543	arg1	analysis					556:563	MALDI-MS based N-glycomic analysis	530:563	MALDI-MS based N-glycomic analysis	530:563	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32270793	8	53	theme	fucosylation	1332:1343	arg1	pattern					1345:1351	the overall fucosylation pattern	1320:1351	the overall fucosylation pattern	1320:1351	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	4	54	theme	MALDI-MS	530:537	arg1	analysis					556:563	MALDI-MS based N-glycomic analysis	530:563	MALDI-MS based N-glycomic analysis	530:563	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32270793	5	55	theme	macrophages	720:730	arg1	infection					707:715	infection	707:715	infection of macrophages by M. bovis BCG	707:746	In contrast, infection of macrophages by M. bovis BCG did not modify the glycosphingolipids composition of macrophages.					
32270793	6	56	theme	increased	902:910	arg1	due					929:931	due	929:931	due	929:931	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	6	56	theme	increased	902:910	arg1	fucosylation					912:923	the increased fucosylation	898:923	the increased fucosylation	898:923	Further nano-LC-MSn glycotope-centric analysis of total N-glycans demonstrated that the increased fucosylation was due to an increased expression of the Lex (Galβ1-4[Fucα1-3]GlcNAc) epitope, also known as stage-specific embryonic antigen-1.					
32270793	7	57	theme	fucose	1189:1194	arg1	lectin					1205:1210	an α1,3-linked fucose specific lectin	1174:1210	an α1,3-linked fucose specific lectin	1174:1210	Modification of the surface expression of Lex was further confirmed in both MDM and THP-1 cells by FACS analysis using an α1,3-linked fucose specific lectin.					
32270793	3	58	theme	monocyte-derived	464:479	arg1	MDM					494:496	MDM	494:496	MDM	494:496	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	3	58	theme	monocyte-derived	464:479	arg1	macrophages					481:491	human primary blood monocyte-derived macrophages	444:491	human primary blood monocyte-derived macrophages (MDM)	444:497	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	9	59	theme	infection-triggered	1496:1514	arg1	remodeling					1529:1538	infection-triggered cell-surface remodeling	1496:1538	infection-triggered cell-surface remodeling of macrophages	1496:1553	These results pave the way toward the understanding of infection-triggered cell-surface remodeling of macrophages.					
32270793	7	60	theme	Lex	1097:1099	arg1	expression					1083:1092	the surface expression	1071:1092	the surface expression of Lex	1071:1099	Modification of the surface expression of Lex was further confirmed in both MDM and THP-1 cells by FACS analysis using an α1,3-linked fucose specific lectin.					
32270793	8	61	theme	mycobacterial	1233:1245	arg1	Pam3Lp19					1259:1266	the mycobacterial lipopeptide Pam3Lp19	1229:1266	the mycobacterial lipopeptide Pam3Lp19	1229:1266	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	8	61	theme	mycobacterial	1233:1245	arg1	agonist					1272:1278	an agonist	1269:1278	an agonist of toll-like receptor 2	1269:1302	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	2	62	dep	cell	311:314	arg1	adhesion					331:338	adhesion	331:338	adhesion	331:338	Indeed, the alteration of the cell-surface glycosylation status may affect the binding of lectins involved in cell activation and adhesion.					
32270793	2	62	dep	cell	311:314	arg1	activation					316:325	activation	316:325	activation	316:325	Indeed, the alteration of the cell-surface glycosylation status may affect the binding of lectins involved in cell activation and adhesion.					
32270793	8	63	theme	infection	1378:1386	arg1	process					1388:1394	the infection process	1374:1394	the infection process	1374:1394	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	7	64	theme	THP-1	1139:1143	arg1	cells					1145:1149	THP-1 cells	1139:1149	THP-1 cells	1139:1149	Modification of the surface expression of Lex was further confirmed in both MDM and THP-1 cells by FACS analysis using an α1,3-linked fucose specific lectin.					
32270793	4	65	theme	type	678:681	arg1	N-glycans					683:691	biantennary and multifucosylated complex type N-glycans	637:691	biantennary and multifucosylated complex type N-glycans	637:691	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32270793	2	66	theme	status	258:263	arg1	alteration					213:222	the alteration	209:222	the alteration of the cell-surface glycosylation status	209:263	Indeed, the alteration of the cell-surface glycosylation status may affect the binding of lectins involved in cell activation and adhesion.					
32270793	4	67	theme	complex	670:676	arg1	N-glycans					683:691	biantennary and multifucosylated complex type N-glycans	637:691	biantennary and multifucosylated complex type N-glycans	637:691	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32270793	3	68	theme	macrophage-cell	503:517	arg1	THP1					524:527	macrophage-cell line THP1	503:527	macrophage-cell line THP1	503:527	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	3	69	theme	M.	382:383	arg1	BCG					391:393	M. bovis BCG	382:393	M. bovis BCG	382:393	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	8	70	theme	toll-like	1283:1291	arg1	receptor					1293:1300	toll-like receptor 2	1283:1302	toll-like receptor 2	1283:1302	Activation with the mycobacterial lipopeptide Pam3Lp19, an agonist of toll-like receptor 2, did not modify the overall fucosylation pattern, which suggests that the infection process is required to modify surface glycosylation.					
32270793	2	71	theme	glycosylation	244:256	arg1	status					258:263	the cell-surface glycosylation status	227:263	the cell-surface glycosylation status	227:263	Indeed, the alteration of the cell-surface glycosylation status may affect the binding of lectins involved in cell activation and adhesion.					
32270793	3	72	theme	line	519:522	arg1	THP1					524:527	macrophage-cell line THP1	503:527	macrophage-cell line THP1	503:527	Herein, we demonstrate that infection by M. bovis BCG induces the remodeling of the N-glycomes of both human primary blood monocyte-derived macrophages (MDM) and macrophage-cell line THP1.					
32270793	4	73	theme	biantennary	637:647	arg1	N-glycans					683:691	biantennary and multifucosylated complex type N-glycans	637:691	biantennary and multifucosylated complex type N-glycans	637:691	MALDI-MS based N-glycomic analysis established that mycobacterial infection induced increased synthesis of biantennary and multifucosylated complex type N-glycans.					
32515505	3	0	from	acid	501:504	arg1	PPe-S					528:532	PPe-S	528:532	PPe-S	528:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	12	1	from	level	2018:2022	arg1	elegans					2030:2036	C. elegans	2027:2036	C. elegans	2027:2036	In this study, we proved that the polysaccharide derived from pumpkin could effectively ameliorate the OS level in C. elegans, including decreasing the damage of biofilm and ROS level.					
32515505	14	2	theme	diabetes	2308:2315	arg1	prevention					2317:2326	diabetes prevention	2308:2326	diabetes prevention	2308:2326	In other words, this research can be applied to diabetes prevention and other diseases induced by OS.					
32515505	2	3	theme	study	285:289	arg1	aim					273:275	The aim	269:275	The aim of this study	269:289	The aim of this study was to explore the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans.					
32515505	1	4	theme	polysaccharides	188:202	arg1	process					169:175	the acid hydrolysis process	149:175	the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design	149:266	Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design.					
32515505	4	5	theme	oxygen	794:799	arg1	ROS					810:812	ROS	810:812	ROS	810:812	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	4	5	theme	oxygen	794:799	arg1	species					801:807	reactive oxygen species	785:807	reactive oxygen species (ROS) level	785:819	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	6	6	theme	PPe-S	1081:1085	arg1	superior					1091:1098	superior	1091:1098	superior	1091:1098	Moreover, the antioxidant effect of PPe-S was superior to PPe at the concentration of 4.0 mg/ml.					
32515505	6	6	theme	PPe-S	1081:1085	arg1	effect					1071:1076	the antioxidant effect	1055:1076	the antioxidant effect of PPe-S	1055:1085	Moreover, the antioxidant effect of PPe-S was superior to PPe at the concentration of 4.0 mg/ml.					
32515505	4	7	theme	antioxidant	635:645	arg1	ability					647:653	antioxidant ability	635:653	antioxidant ability	635:653	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	6	8	theme	4.0 mg/ml	1131:1139	arg1	concentration					1114:1126	the concentration	1110:1126	the concentration of 4.0 mg/ml	1110:1139	Moreover, the antioxidant effect of PPe-S was superior to PPe at the concentration of 4.0 mg/ml.					
32515505	7	9	theme	agents	1310:1315	arg1	generation					1269:1278	the generation	1265:1278	the generation of intracellular free radical agents	1265:1315	In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents.					
32515505	11	10	from	level	1836:1840	arg1	tissue					1854:1859	tissue	1854:1859	tissue	1854:1859	Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well.					
32515505	11	10	from	level	1836:1840	arg1	cell					1845:1848	cell	1845:1848	cell	1845:1848	Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well.					
32515505	13	11	theme	high	2140:2143	arg1	potential					2145:2153	a high potential	2138:2153	a high potential for pumpkin-derived polysaccharide and its hydrolyzates to be a bioactive component to prevent diabetes	2138:2257	Therefore, our study shows that there is a high potential for pumpkin-derived polysaccharide and its hydrolyzates to be a bioactive component to prevent diabetes.					
32515505	11	12	link	plant-derived	1753:1765	arg1	polysaccharide					1767:1780	plant-derived polysaccharide	1753:1780	plant-derived polysaccharide	1753:1780	Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well.					
32515505	8	13	theme	important	1354:1362	arg1	role					1364:1367	an important role	1351:1367	an important role	1351:1367	PRACTICAL APPLICATIONS: OS plays an important role in the pathogenesis of metabolic diseases, including type 2 diabetes.					
32515505	7	14	link	derived	1187:1193	arg1	hydrolyzates					1195:1206	the derived hydrolyzates	1183:1206	the derived hydrolyzates from PPe	1183:1215	In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents.					
32515505	3	15	from	%	551:551	arg1	analysis					442:449	composition analysis	430:449	composition analysis	430:449	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	8	16	theme	metabolic	1392:1400	arg1	diabetes					1429:1436	type 2 diabetes	1422:1436	type 2 diabetes	1422:1436	PRACTICAL APPLICATIONS: OS plays an important role in the pathogenesis of metabolic diseases, including type 2 diabetes.					
32515505	8	16	theme	metabolic	1392:1400	arg1	diseases					1402:1409	metabolic diseases	1392:1409	metabolic diseases	1392:1409	PRACTICAL APPLICATIONS: OS plays an important role in the pathogenesis of metabolic diseases, including type 2 diabetes.					
32515505	10	17	theme	effects	1689:1695	arg1	kind					1676:1679	a kind	1674:1679	a kind of side effects	1674:1695	Current studies have shown that all diabetes drugs have a kind of side effects.					
32515505	1	18	theme	hydrolysis	158:167	arg1	process					169:175	the acid hydrolysis process	149:175	the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design	149:266	Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design.					
32515505	0	19	theme	Caenorhabditis	86:99	arg1	model					109:113	Caenorhabditis elegans model	86:113	Caenorhabditis elegans model	86:113	The antioxidant capacity evaluation of polysaccharide hydrolyzates from pumpkin using Caenorhabditis elegans model.					
32515505	8	20	theme	type	1422:1425	arg1	diabetes					1429:1436	type 2 diabetes	1422:1436	type 2 diabetes	1422:1436	PRACTICAL APPLICATIONS: OS plays an important role in the pathogenesis of metabolic diseases, including type 2 diabetes.					
32515505	12	21	theme	ROS	2086:2088	arg1	level					2090:2094	ROS level	2086:2094	ROS level	2086:2094	In this study, we proved that the polysaccharide derived from pumpkin could effectively ameliorate the OS level in C. elegans, including decreasing the damage of biofilm and ROS level.					
32515505	6	22	from	concentration	1114:1126	arg1	superior					1091:1098	superior	1091:1098	superior	1091:1098	Moreover, the antioxidant effect of PPe-S was superior to PPe at the concentration of 4.0 mg/ml.					
32515505	6	22	from	concentration	1114:1126	arg1	effect					1071:1076	the antioxidant effect	1055:1076	the antioxidant effect of PPe-S	1055:1085	Moreover, the antioxidant effect of PPe-S was superior to PPe at the concentration of 4.0 mg/ml.					
32515505	11	23	theme	diabetes	1885:1892	arg1	symptoms					1894:1901	the diabetes symptoms	1881:1901	the diabetes symptoms as well	1881:1909	Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well.					
32515505	11	24	theme	notable	1788:1794	arg1	effect					1809:1814	a notable hypoglycemic effect	1786:1814	a notable hypoglycemic effect	1786:1814	Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well.					
32515505	4	25	dep	significantly	848:860	arg1	p < .05					863:869	p < .05	863:869	p < .05	863:869	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	11	26	contain	had	1782:1784	arg2	effect					1809:1814	a notable hypoglycemic effect	1786:1814	a notable hypoglycemic effect	1786:1814	Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well.					
32515505	11	26	contain	had	1782:1784	arg1	polysaccharide					1767:1780	plant-derived polysaccharide	1753:1780	plant-derived polysaccharide	1753:1780	Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well.					
32515505	13	27	link	pumpkin-derived	2159:2173	arg1	polysaccharide					2175:2188	pumpkin-derived polysaccharide	2159:2188	pumpkin-derived polysaccharide	2159:2188	Therefore, our study shows that there is a high potential for pumpkin-derived polysaccharide and its hydrolyzates to be a bioactive component to prevent diabetes.					
32515505	4	28	theme	oxidative	698:706	arg1	stress					708:713	the oxidative stress	694:713	the oxidative stress (OS) induced by methyl viologen	694:745	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	4	28	theme	oxidative	698:706	arg1	OS					716:717	OS	716:717	OS	716:717	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	3	29	from	%	598:598	arg1	analysis					442:449	composition analysis	430:449	composition analysis	430:449	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	3	30	theme	composition	430:440	arg1	analysis					442:449	composition analysis	430:449	composition analysis	430:449	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	0	31	dep	Caenorhabditis	86:99	arg1	elegans					101:107	elegans	101:107	elegans	101:107	The antioxidant capacity evaluation of polysaccharide hydrolyzates from pumpkin using Caenorhabditis elegans model.					
32515505	12	32	theme	biofilm	2074:2080	arg1	damage					2064:2069	the damage	2060:2069	the damage of biofilm and ROS level	2060:2094	In this study, we proved that the polysaccharide derived from pumpkin could effectively ameliorate the OS level in C. elegans, including decreasing the damage of biofilm and ROS level.					
32515505	3	33	theme	total	472:476	arg1	sugar					478:482	total sugar	472:482	total sugar	472:482	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	0	34	theme	capacity	16:23	arg1	evaluation					25:34	The antioxidant capacity evaluation	0:34	The antioxidant capacity evaluation of polysaccharide	0:52	The antioxidant capacity evaluation of polysaccharide hydrolyzates from pumpkin using Caenorhabditis elegans model.					
32515505	1	35	theme	previous	120:127	arg1	study					129:133	Our previous study	116:133	Our previous study	116:133	Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design.					
32515505	7	36	theme	protective	1221:1230	arg1	effects					1232:1238	protective effects	1221:1238	protective effects	1221:1238	In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents.					
32515505	3	37	from	%	580:580	arg1	analysis					442:449	composition analysis	430:449	composition analysis	430:449	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	13	38	theme	pumpkin-derived	2159:2173	arg1	polysaccharide					2175:2188	pumpkin-derived polysaccharide	2159:2188	pumpkin-derived polysaccharide	2159:2188	Therefore, our study shows that there is a high potential for pumpkin-derived polysaccharide and its hydrolyzates to be a bioactive component to prevent diabetes.					
32515505	2	39	theme	PPe	341:343	arg1	ability					330:336	the in vivo-antioxidant ability	306:336	the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans	306:424	The aim of this study was to explore the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans.					
32515505	9	40	theme	human	1518:1522	arg1	health					1526:1531	human's health	1518:1531	human's health	1518:1531	It is widely acknowledged that diabetes and its complications pose a threat to human's health, and the number of people with diabetes will expand to 640 million in the 2040 year.					
32515505	3	41	from	sugar	478:482	arg1	PPe-S					528:532	PPe-S	528:532	PPe-S	528:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	1	42	with	polysaccharides	188:202	arg1	ability					226:232	scavenging ability	215:232	scavenging ability	215:232	Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design.					
32515505	5	43	theme	stress-resistance	918:934	arg1	catalase					974:981	catalase	974:981	catalase (CAT)	974:987	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	5	43	theme	stress-resistance	918:934	arg1	dismutase					1004:1012	superoxide dismutase	993:1012	superoxide dismutase (SOD)	993:1018	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	5	43	theme	stress-resistance	918:934	arg1	enzymes					956:962	the stress-resistance related antioxidant enzymes	914:962	the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD)	914:1018	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	10	44	theme	Current	1618:1624	arg1	studies					1626:1632	Current studies	1618:1632	Current studies	1618:1632	Current studies have shown that all diabetes drugs have a kind of side effects.					
32515505	7	45	dep	intracellular	1283:1295	arg1	free					1297:1300	free	1297:1300	free	1297:1300	In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents.					
32515505	1	46	theme	central	243:249	arg1	design					261:266	central composite design	243:266	central composite design	243:266	Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design.					
32515505	14	47	theme	other	2332:2336	arg1	diseases					2338:2345	other diseases	2332:2345	other diseases induced by OS	2332:2359	In other words, this research can be applied to diabetes prevention and other diseases induced by OS.					
32515505	5	48	theme	antioxidant	944:954	arg1	catalase					974:981	catalase	974:981	catalase (CAT)	974:987	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	5	48	theme	antioxidant	944:954	arg1	dismutase					1004:1012	superoxide dismutase	993:1012	superoxide dismutase (SOD)	993:1018	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	5	48	theme	antioxidant	944:954	arg1	enzymes					956:962	the stress-resistance related antioxidant enzymes	914:962	the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD)	914:1018	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	2	49	theme	pumpkin	349:355	arg1	acid-hydrolysis					373:387	pumpkin polysaccharides acid-hydrolysis	349:387	pumpkin polysaccharides acid-hydrolysis	349:387	The aim of this study was to explore the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans.					
32515505	4	50	theme	species	801:807	arg1	level					815:819	reactive oxygen species (ROS) level	785:819	reactive oxygen species (ROS) level	785:819	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	9	51	theme	people	1552:1557	arg1	number					1542:1547	the number	1538:1547	the number of people with diabetes	1538:1571	It is widely acknowledged that diabetes and its complications pose a threat to human's health, and the number of people with diabetes will expand to 640 million in the 2040 year.					
32515505	9	52	with	people	1552:1557	arg1	diabetes					1564:1571	diabetes	1564:1571	diabetes	1564:1571	It is widely acknowledged that diabetes and its complications pose a threat to human's health, and the number of people with diabetes will expand to 640 million in the 2040 year.					
32515505	3	53	theme	groups	518:523	arg1	%					551:551	87.03 ± 1.21%	539:551	87.03 ± 1.21%	539:551	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	3	53	theme	groups	518:523	arg1	constituents					456:467	the constituents	452:467	the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S	452:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	1	54	theme	pumpkin	180:186	arg1	PPe					205:207	PPe	205:207	PPe	205:207	Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design.					
32515505	1	54	theme	pumpkin	180:186	arg1	polysaccharides					188:202	pumpkin polysaccharides	180:202	pumpkin polysaccharides (PPe) with scavenging ability	180:232	Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design.					
32515505	12	55	attach	derived	1961:1967	arg2	polysaccharide					1946:1959	the polysaccharide	1942:1959	the polysaccharide derived from pumpkin	1942:1980	In this study, we proved that the polysaccharide derived from pumpkin could effectively ameliorate the OS level in C. elegans, including decreasing the damage of biofilm and ROS level.					
32515505	12	55	attach	derived	1961:1967	arg1	pumpkin					1974:1980	pumpkin	1974:1980	pumpkin	1974:1980	In this study, we proved that the polysaccharide derived from pumpkin could effectively ameliorate the OS level in C. elegans, including decreasing the damage of biofilm and ROS level.					
32515505	3	56	from	protein	485:491	arg1	PPe-S					528:532	PPe-S	528:532	PPe-S	528:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	4	57	theme	reactive	785:792	arg1	ROS					810:812	ROS	810:812	ROS	810:812	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	4	57	theme	reactive	785:792	arg1	species					801:807	reactive oxygen species	785:807	reactive oxygen species (ROS) level	785:819	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	8	58	theme	PRACTICAL	1318:1326	arg1	APPLICATIONS					1328:1339	PRACTICAL APPLICATIONS	1318:1339	PRACTICAL APPLICATIONS: OS plays an important role in the pathogenesis of metabolic diseases, including type 2 diabetes.	1318:1437	PRACTICAL APPLICATIONS: OS plays an important role in the pathogenesis of metabolic diseases, including type 2 diabetes.					
32515505	10	59	theme	diabetes	1654:1661	arg1	drugs					1663:1667	all diabetes drugs	1650:1667	all diabetes drugs	1650:1667	Current studies have shown that all diabetes drugs have a kind of side effects.					
32515505	2	60	theme	acid-hydrolysis	373:387	arg1	ability					330:336	the in vivo-antioxidant ability	306:336	the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans	306:424	The aim of this study was to explore the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans.					
32515505	6	61	theme	antioxidant	1059:1069	arg1	superior					1091:1098	superior	1091:1098	superior	1091:1098	Moreover, the antioxidant effect of PPe-S was superior to PPe at the concentration of 4.0 mg/ml.					
32515505	6	61	theme	antioxidant	1059:1069	arg1	effect					1071:1076	the antioxidant effect	1055:1076	the antioxidant effect of PPe-S	1055:1085	Moreover, the antioxidant effect of PPe-S was superior to PPe at the concentration of 4.0 mg/ml.					
32515505	1	62	theme	acid	153:156	arg1	process					169:175	the acid hydrolysis process	149:175	the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design	149:266	Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design.					
32515505	3	63	theme	acid	501:504	arg1	%					551:551	87.03 ± 1.21%	539:551	87.03 ± 1.21%	539:551	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	3	63	theme	acid	501:504	arg1	constituents					456:467	the constituents	452:467	the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S	452:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	10	64	theme	side	1684:1687	arg1	effects					1689:1695	side effects	1684:1695	side effects	1684:1695	Current studies have shown that all diabetes drugs have a kind of side effects.					
32515505	1	65	theme	scavenging	215:224	arg1	ability					226:232	scavenging ability	215:232	scavenging ability	215:232	Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design.					
32515505	7	66	theme	radical	1302:1308	arg1	agents					1310:1315	intracellular free radical agents	1283:1315	intracellular free radical agents	1283:1315	In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents.					
32515505	7	67	contain	had	1217:1219	arg2	effects					1232:1238	protective effects	1221:1238	protective effects	1221:1238	In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents.					
32515505	7	67	contain	had	1217:1219	arg1	hydrolyzates					1195:1206	the derived hydrolyzates	1183:1206	the derived hydrolyzates from PPe	1183:1215	In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents.					
32515505	11	68	theme	OS	1833:1834	arg1	level					1836:1840	the OS level	1829:1840	the OS level in cell and tissue	1829:1859	Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well.					
32515505	7	69	theme	intracellular	1283:1295	arg1	agents					1310:1315	intracellular free radical agents	1283:1315	intracellular free radical agents	1283:1315	In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents.					
32515505	4	70	theme	oxidative	827:835	arg1	conditions					837:846	oxidative conditions	827:846	oxidative conditions	827:846	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	8	71	theme	diseases	1402:1409	arg1	pathogenesis					1376:1387	the pathogenesis	1372:1387	the pathogenesis of metabolic diseases, including type 2 diabetes	1372:1436	PRACTICAL APPLICATIONS: OS plays an important role in the pathogenesis of metabolic diseases, including type 2 diabetes.					
32515505	5	72	theme	superoxide	993:1002	arg1	SOD					1015:1017	SOD	1015:1017	SOD	1015:1017	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	5	72	theme	superoxide	993:1002	arg1	dismutase					1004:1012	superoxide dismutase	993:1012	superoxide dismutase (SOD)	993:1018	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	8	73	dep	APPLICATIONS	1328:1339	arg1	plays					1345:1349	plays	1345:1349	plays an important role in the pathogenesis of metabolic diseases, including type 2 diabetes	1345:1436	PRACTICAL APPLICATIONS: OS plays an important role in the pathogenesis of metabolic diseases, including type 2 diabetes.					
32515505	4	74	theme	ability	647:653	arg1	results					624:630	results	624:630	results of antioxidant ability	624:653	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	3	75	from	groups	518:523	arg1	PPe-S					528:532	PPe-S	528:532	PPe-S	528:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	9	76	from	million	1592:1598	arg1	2040 year					1607:1615	the 2040 year	1603:1615	the 2040 year	1603:1615	It is widely acknowledged that diabetes and its complications pose a threat to human's health, and the number of people with diabetes will expand to 640 million in the 2040 year.					
32515505	3	77	from	PPe-S	528:532	arg1	%					551:551	87.03 ± 1.21%	539:551	87.03 ± 1.21%	539:551	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	3	77	from	PPe-S	528:532	arg1	constituents					456:467	the constituents	452:467	the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S	452:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	12	78	theme	OS	2015:2016	arg1	level					2018:2022	the OS level	2011:2022	the OS level in C. elegans	2011:2036	In this study, we proved that the polysaccharide derived from pumpkin could effectively ameliorate the OS level in C. elegans, including decreasing the damage of biofilm and ROS level.					
32515505	2	79	theme	vivo-antioxidant	313:328	arg1	ability					330:336	the in vivo-antioxidant ability	306:336	the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans	306:424	The aim of this study was to explore the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans.					
32515505	10	80	contain	have	1669:1672	arg1	drugs					1663:1667	all diabetes drugs	1650:1667	all diabetes drugs	1650:1667	Current studies have shown that all diabetes drugs have a kind of side effects.					
32515505	10	80	contain	have	1669:1672	arg2	kind					1676:1679	a kind	1674:1679	a kind of side effects	1674:1695	Current studies have shown that all diabetes drugs have a kind of side effects.					
32515505	12	81	theme	level	2090:2094	arg1	damage					2064:2069	the damage	2060:2069	the damage of biofilm and ROS level	2060:2094	In this study, we proved that the polysaccharide derived from pumpkin could effectively ameliorate the OS level in C. elegans, including decreasing the damage of biofilm and ROS level.					
32515505	11	82	theme	hypoglycemic	1796:1807	arg1	effect					1809:1814	a notable hypoglycemic effect	1786:1814	a notable hypoglycemic effect	1786:1814	Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well.					
32515505	0	83	theme	antioxidant	4:14	arg1	capacity					16:23	The antioxidant capacity	0:23	The antioxidant capacity evaluation of polysaccharide	0:52	The antioxidant capacity evaluation of polysaccharide hydrolyzates from pumpkin using Caenorhabditis elegans model.					
32515505	11	84	theme	plant-derived	1753:1765	arg1	polysaccharide					1767:1780	plant-derived polysaccharide	1753:1780	plant-derived polysaccharide	1753:1780	Fortunately, researchers have found and confirmed that plant-derived polysaccharide had a notable hypoglycemic effect via reducing the OS level in cell and tissue, and could decrease the diabetes symptoms as well.					
32515505	2	85	dep	vivo-antioxidant	313:328	arg1	in					310:311	in	310:311	in	310:311	The aim of this study was to explore the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans.					
32515505	3	86	from	%	565:565	arg1	analysis					442:449	composition analysis	430:449	composition analysis	430:449	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	3	87	theme	sugar	478:482	arg1	%					551:551	87.03 ± 1.21%	539:551	87.03 ± 1.21%	539:551	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	3	87	theme	sugar	478:482	arg1	constituents					456:467	the constituents	452:467	the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S	452:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	0	88	theme	polysaccharide	39:52	arg1	evaluation					25:34	The antioxidant capacity evaluation	0:34	The antioxidant capacity evaluation of polysaccharide	0:52	The antioxidant capacity evaluation of polysaccharide hydrolyzates from pumpkin using Caenorhabditis elegans model.					
32515505	13	89	theme	bioactive	2219:2227	arg1	component					2229:2237	a bioactive component	2217:2237	a bioactive component to prevent diabetes	2217:2257	Therefore, our study shows that there is a high potential for pumpkin-derived polysaccharide and its hydrolyzates to be a bioactive component to prevent diabetes.					
32515505	3	90	from	constituents	456:467	arg1	PPe-S					528:532	PPe-S	528:532	PPe-S	528:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	7	91	from	PPe	1213:1215	arg1	hydrolyzates					1195:1206	the derived hydrolyzates	1183:1206	the derived hydrolyzates from PPe	1183:1215	In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents.					
32515505	7	92	theme	derived	1187:1193	arg1	hydrolyzates					1195:1206	the derived hydrolyzates	1183:1206	the derived hydrolyzates from PPe	1183:1215	In summary, this study demonstrated that the derived hydrolyzates from PPe had protective effects on the damage induced by the generation of intracellular free radical agents.					
32515505	2	93	theme	polysaccharides	357:371	arg1	acid-hydrolysis					373:387	pumpkin polysaccharides acid-hydrolysis	349:387	pumpkin polysaccharides acid-hydrolysis	349:387	The aim of this study was to explore the in vivo-antioxidant ability of PPe and pumpkin polysaccharides acid-hydrolysis (PPe-S) using Caenorhabditis elegans.					
32515505	5	94	dep	significantly	1020:1032	arg1	p < .05					1035:1041	p < .05	1035:1041	p < .05	1035:1041	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	4	95	theme	worms	767:771	arg1	lifespan					755:762	lifespan	755:762	lifespan of worms	755:771	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	3	96	theme	uronic	494:499	arg1	acid					501:504	uronic acid	494:504	uronic acid	494:504	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	4	97	theme	methyl	731:736	arg1	viologen					738:745	methyl viologen	731:745	methyl viologen	731:745	Besides, results of antioxidant ability showed that PPe and PPe-S could reduce the oxidative stress (OS) induced by methyl viologen, extend lifespan of worms, and reduce reactive oxygen species (ROS) level under oxidative conditions significantly (p < .05).					
32515505	14	98	theme	other	2263:2267	arg1	words					2269:2273	other words	2263:2273	other words	2263:2273	In other words, this research can be applied to diabetes prevention and other diseases induced by OS.					
32515505	3	99	theme	protein	485:491	arg1	%					551:551	87.03 ± 1.21%	539:551	87.03 ± 1.21%	539:551	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	3	99	theme	protein	485:491	arg1	constituents					456:467	the constituents	452:467	the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S	452:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	5	100	theme	related	936:942	arg1	catalase					974:981	catalase	974:981	catalase (CAT)	974:987	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	5	100	theme	related	936:942	arg1	dismutase					1004:1012	superoxide dismutase	993:1012	superoxide dismutase (SOD)	993:1018	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	5	100	theme	related	936:942	arg1	enzymes					956:962	the stress-resistance related antioxidant enzymes	914:962	the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD)	914:1018	Furthermore, PPe and PPe-S could enhance the stress-resistance related antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) significantly (p < .05).					
32515505	3	101	theme	sulfur	511:516	arg1	groups					518:523	sulfur groups	511:523	sulfur groups in PPe-S	511:532	In composition analysis, the constituents of total sugar, protein, uronic acid, and sulfur groups in PPe-S were 87.03 ± 1.21%, 1.25 ± 0.78%, 37.61 ± 0.97%, and 0.14 ± 0.04%, respectively.					
32515505	1	102	theme	composite	251:259	arg1	design					261:266	central composite design	243:266	central composite design	243:266	Our previous study has optimized the acid hydrolysis process of pumpkin polysaccharides (PPe) with scavenging ability based on central composite design.					
34893272	0	0	theme	Panax	106:110	arg1	medicines					119:127	six Panax herbal medicines	102:127	six Panax herbal medicines	102:127	Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines.					
34893272	7	1	theme	quality	1246:1252	arg1	control					1254:1260	quality control	1246:1260	quality control of ginseng	1246:1271	These new evidences may provide support for the supplementary role of polysaccharides in quality control of ginseng.					
34893272	5	2	theme	weight	880:885	arg1	distribution					887:898	molecular weight distribution	870:898	molecular weight distribution	870:898	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	3	3	theme	chemical	415:422	arg1	approaches					439:448	both chemical and biological approaches	410:448	both chemical and biological approaches	410:448	major (ZZS), was performed by both chemical and biological approaches.					
34893272	4	4	theme	hydrolyzed	501:510	arg1	oligosaccharide					512:526	the hydrolyzed oligosaccharide and monosaccharide levels	497:552	oligosaccharide	512:526	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	7	5	theme	polysaccharides	1227:1241	arg1	role					1219:1222	the supplementary role	1201:1222	the supplementary role of polysaccharides in quality control of ginseng	1201:1271	These new evidences may provide support for the supplementary role of polysaccharides in quality control of ginseng.					
34893272	4	6	theme	mitochondrial	709:721	arg1	function					723:730	mitochondrial function	709:730	mitochondrial function	709:730	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	4	7	theme	cell	690:693	arg1	viability					695:703	cell viability	690:703	cell viability	690:703	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	4	8	theme	monosaccharide	532:545	arg1	levels					547:552	the hydrolyzed oligosaccharide and monosaccharide levels	497:552	levels	547:552	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	0	9	theme	herbal	112:117	arg1	medicines					119:127	six Panax herbal medicines	102:127	six Panax herbal medicines	102:127	Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines.					
34893272	6	10	theme	mitochondrial	1132:1144	arg1	functions					1146:1154	mitochondrial functions	1132:1154	mitochondrial functions	1132:1154	RG polysaccharides (25/50/100 μg/mL) showed significant cardiomyocyte protection by regulating mitochondrial functions.					
34893272	4	11	from	oligosaccharide	512:526	arg1	fingerprinting					460:473	Holistic fingerprinting	451:473	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels	451:552	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	6	12	theme	significant	1081:1091	arg1	protection					1107:1116	significant cardiomyocyte protection	1081:1116	significant cardiomyocyte protection	1081:1116	RG polysaccharides (25/50/100 μg/mL) showed significant cardiomyocyte protection by regulating mitochondrial functions.					
34893272	4	13	from	models	615:620	arg1	cells					630:634	H9c2 cells	625:634	H9c2 cells	625:634	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	4	14	theme	OGD/R	609:613	arg1	models					615:620	OGD and OGD/R models	601:620	OGD and OGD/R models on H9c2 cells	601:634	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	7	15	theme	new	1163:1165	arg1	evidences					1167:1175	These new evidences	1157:1175	These new evidences	1157:1175	These new evidences may provide support for the supplementary role of polysaccharides in quality control of ginseng.					
34893272	5	16	from	species	766:772	arg1	Polysaccharides					733:747	Polysaccharides	733:747	Polysaccharides from six ginseng species	733:772	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	5	17	theme	monosaccharide	965:978	arg1	compositions					980:991	monosaccharide compositions	965:991	monosaccharide compositions	965:991	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	6	18	theme	cardiomyocyte	1093:1105	arg1	protection					1107:1116	significant cardiomyocyte protection	1081:1116	significant cardiomyocyte protection	1081:1116	RG polysaccharides (25/50/100 μg/mL) showed significant cardiomyocyte protection by regulating mitochondrial functions.					
34893272	5	19	theme	polysaccharides	1006:1020	arg1	compositions					980:991	monosaccharide compositions	965:991	monosaccharide compositions	965:991	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	5	20	theme	weak	845:848	arg1	differentiations					850:865	weak differentiations	845:865	weak differentiations in molecular weight distribution and oligosaccharide profiles	845:927	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	0	21	theme	Multi-level	0:10	arg1	fingerprinting					12:25	Multi-level fingerprinting	0:25	Multi-level fingerprinting	0:25	Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines.					
34893272	1	22	from	role	134:137	arg1	control					169:175	quality control	161:175	quality control of ginseng	161:186	The role of polysaccharides in quality control of ginseng is underestimated.					
34893272	0	23	theme	cardiomyocyte	31:43	arg1	evaluation					56:65	cardiomyocyte protection evaluation	31:65	cardiomyocyte protection evaluation	31:65	Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines.					
34893272	4	24	from	effects	679:685	arg1	viability					695:703	cell viability	690:703	cell viability	690:703	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	4	24	from	effects	679:685	arg1	function					723:730	mitochondrial function	709:730	mitochondrial function	709:730	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	2	25	theme	P.	299:300	arg1	PN					315:316	PN	315:316	PN	315:316	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	25	theme	P.	299:300	arg1	notoginseng					302:312	P. notoginseng	299:312	P. notoginseng (PN)	299:317	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	25	theme	P.	299:300	arg1	comparison					219:228	Large-scale comparison	207:228	Large-scale comparison on the polysaccharides of Panax ginseng (PG)	207:273	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	5	26	theme	content	795:801	arg1	RG > PG/ZZS/ZJS/PQ > PN					815:837	RG > PG/ZZS/ZJS/PQ > PN	815:837	RG > PG/ZZS/ZJS/PQ > PN	815:837	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	5	26	theme	content	795:801	arg1	difference					803:812	remarkable content difference	784:812	remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN)	784:838	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	2	27	theme	Large-scale	207:217	arg1	japonicus					341:349	P. japonicus	338:349	P. japonicus (ZJS)	338:355	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	27	theme	Large-scale	207:217	arg1	notoginseng					302:312	P. notoginseng	299:312	P. notoginseng (PN)	299:317	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	27	theme	Large-scale	207:217	arg1	japonicus					365:373	P. japonicus	362:373	P. japonicus	362:373	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	27	theme	Large-scale	207:217	arg1	ginseng					324:330	Red ginseng	320:330	Red ginseng (RG)	320:335	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	27	theme	Large-scale	207:217	arg1	quinquefolius					279:291	P. quinquefolius	276:291	P. quinquefolius (PQ)	276:296	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	27	theme	Large-scale	207:217	arg1	comparison					219:228	Large-scale comparison	207:228	Large-scale comparison on the polysaccharides of Panax ginseng (PG)	207:273	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	1	28	theme	ginseng	180:186	arg1	control					169:175	quality control	161:175	quality control of ginseng	161:186	The role of polysaccharides in quality control of ginseng is underestimated.					
34893272	5	29	theme	remarkable	784:793	arg1	RG > PG/ZZS/ZJS/PQ > PN					815:837	RG > PG/ZZS/ZJS/PQ > PN	815:837	RG > PG/ZZS/ZJS/PQ > PN	815:837	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	5	29	theme	remarkable	784:793	arg1	difference					803:812	remarkable content difference	784:812	remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN)	784:838	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	6	30	theme	RG	1037:1038	arg1	polysaccharides					1040:1054	RG polysaccharides	1037:1054	RG polysaccharides (25/50/100 μg/mL)	1037:1072	RG polysaccharides (25/50/100 μg/mL) showed significant cardiomyocyte protection by regulating mitochondrial functions.					
34893272	6	30	theme	RG	1037:1038	arg1	25/50/100 μg/mL					1057:1071	25/50/100 μg/mL	1057:1071	25/50/100 μg/mL	1057:1071	RG polysaccharides (25/50/100 μg/mL) showed significant cardiomyocyte protection by regulating mitochondrial functions.					
34893272	5	31	theme	RG	1003:1004	arg1	polysaccharides					1006:1020	PG and RG polysaccharides	996:1020	PG and RG polysaccharides	996:1020	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	4	32	theme	OGD	601:603	arg1	models					615:620	OGD and OGD/R models	601:620	OGD and OGD/R models on H9c2 cells	601:634	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	0	33	theme	protection	45:54	arg1	evaluation					56:65	cardiomyocyte protection evaluation	31:65	cardiomyocyte protection evaluation	31:65	Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines.					
34893272	4	34	used	utilized	554:561	arg2	polysaccharide					478:491	polysaccharide	478:491	polysaccharide	478:491	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	4	34	used	utilized	554:561	arg2	fingerprinting					460:473	Holistic fingerprinting	451:473	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels	451:552	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	4	34	used	utilized	554:561	arg2	oligosaccharide					512:526	the hydrolyzed oligosaccharide and monosaccharide levels	497:552	oligosaccharide	512:526	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	2	35	theme	ginseng	262:268	arg1	polysaccharides					237:251	the polysaccharides	233:251	the polysaccharides of Panax ginseng (PG)	233:273	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	4	36	from	polysaccharide	478:491	arg1	fingerprinting					460:473	Holistic fingerprinting	451:473	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels	451:552	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	0	37	theme	comparing	71:79	arg1	polysaccharides					81:95	comparing polysaccharides	71:95	comparing polysaccharides from six Panax herbal medicines	71:127	Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines.					
34893272	2	38	theme	Panax	256:260	arg1	ginseng					262:268	Panax ginseng	256:268	Panax ginseng (PG)	256:273	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	38	theme	Panax	256:260	arg1	PG					271:272	PG	271:272	PG	271:272	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	39	theme	Red	320:322	arg1	RG					333:334	RG	333:334	RG	333:334	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	39	theme	Red	320:322	arg1	comparison					219:228	Large-scale comparison	207:228	Large-scale comparison on the polysaccharides of Panax ginseng (PG)	207:273	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	2	39	theme	Red	320:322	arg1	ginseng					324:330	Red ginseng	320:330	Red ginseng (RG)	320:335	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	5	40	theme	ginseng	758:764	arg1	species					766:772	six ginseng species	754:772	six ginseng species	754:772	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	5	41	theme	oligosaccharide	904:918	arg1	profiles					920:927	oligosaccharide profiles	904:927	oligosaccharide profiles	904:927	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	4	42	theme	Holistic	451:458	arg1	fingerprinting					460:473	Holistic fingerprinting	451:473	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels	451:552	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	4	43	theme	H9c2	625:628	arg1	cells					630:634	H9c2 cells	625:634	H9c2 cells	625:634	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	1	44	theme	polysaccharides	142:156	arg1	role					134:137	The role	130:137	The role of polysaccharides in quality control of ginseng	130:186	The role of polysaccharides in quality control of ginseng is underestimated.					
34893272	0	45	from	medicines	119:127	arg1	polysaccharides					81:95	comparing polysaccharides	71:95	comparing polysaccharides from six Panax herbal medicines	71:127	Multi-level fingerprinting and cardiomyocyte protection evaluation for comparing polysaccharides from six Panax herbal medicines.					
34893272	2	46	dep	quinquefolius	279:291	arg1	var					375:377	var	375:377	var	375:377	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
34893272	4	47	theme	protective	668:677	arg1	effects					679:685	the protective effects	664:685	the protective effects on cell viability and mitochondrial function	664:730	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	5	48	theme	molecular	870:878	arg1	distribution					887:898	molecular weight distribution	870:898	molecular weight distribution	870:898	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	5	49	theme	PG	996:997	arg1	polysaccharides					1006:1020	PG and RG polysaccharides	996:1020	PG and RG polysaccharides	996:1020	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	5	50	from	differentiations	850:865	arg1	distribution					887:898	molecular weight distribution	870:898	molecular weight distribution	870:898	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	5	50	from	differentiations	850:865	arg1	profiles					920:927	oligosaccharide profiles	904:927	oligosaccharide profiles	904:927	Polysaccharides from six ginseng species exhibited remarkable content difference (RG > PG/ZZS/ZJS/PQ > PN), but weak differentiations in molecular weight distribution and oligosaccharide profiles, while Glc and GalA were richer for monosaccharide compositions of PG and RG polysaccharides, respectively.					
34893272	1	51	theme	quality	161:167	arg1	control					169:175	quality control	161:175	quality control of ginseng	161:186	The role of polysaccharides in quality control of ginseng is underestimated.					
34893272	4	52	theme	chromatography	571:584	arg1	methods					586:592	various chromatography methods	563:592	various chromatography methods	563:592	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	7	53	theme	ginseng	1265:1271	arg1	control					1254:1260	quality control	1246:1260	quality control of ginseng	1246:1271	These new evidences may provide support for the supplementary role of polysaccharides in quality control of ginseng.					
34893272	4	54	theme	various	563:569	arg1	methods					586:592	various chromatography methods	563:592	various chromatography methods	563:592	Holistic fingerprinting at polysaccharide and the hydrolyzed oligosaccharide and monosaccharide levels utilized various chromatography methods, while OGD and OGD/R models on H9c2 cells were introduced to evaluate the protective effects on cell viability and mitochondrial function.					
34893272	7	55	from	role	1219:1222	arg1	control					1254:1260	quality control	1246:1260	quality control of ginseng	1246:1271	These new evidences may provide support for the supplementary role of polysaccharides in quality control of ginseng.					
34893272	3	56	theme	biological	428:437	arg1	approaches					439:448	both chemical and biological approaches	410:448	both chemical and biological approaches	410:448	major (ZZS), was performed by both chemical and biological approaches.					
34893272	7	57	theme	supplementary	1205:1217	arg1	role					1219:1222	the supplementary role	1201:1222	the supplementary role of polysaccharides in quality control of ginseng	1201:1271	These new evidences may provide support for the supplementary role of polysaccharides in quality control of ginseng.					
34893272	2	58	from	comparison	219:228	arg1	polysaccharides					237:251	the polysaccharides	233:251	the polysaccharides of Panax ginseng (PG)	233:273	Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), Red ginseng (RG), P. japonicus (ZJS), and P. japonicus var.					
31954796	4	0	theme	FChNF	482:486	arg1	nanopapers					488:497	FChNF nanopapers	482:497	FChNF nanopapers	482:497	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	7	1	theme	origin	1267:1272	arg1	nanopapers					1225:1234	chitin nanopapers	1218:1234	chitin nanopapers of crustacean (Cancer pagurus) origin	1218:1272	Chitin-glucan nanopaper morphology, mechanical, chemical, and surface properties were studied and compared to chitin nanopapers of crustacean (Cancer pagurus) origin.					
31954796	4	2	from	membranes	561:569	arg1	applicable					515:524	applicable	515:524	applicable	515:524	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	5	3	theme	chitin-glucan	691:703	arg1	nanofibrils					705:715	chitin-glucan nanofibrils	691:715	chitin-glucan nanofibrils	691:715	Here, chitin-glucan nanofibrils were extracted from common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex.					
31954796	6	4	theme	simple	1074:1079	arg1	process					1099:1105	a simple vacuum filtration process	1072:1105	a simple vacuum filtration process	1072:1105	These extracts were readily disintegrated into nanofibre dimensions by a low-energy mechanical blending, thus making the extract dispersion directly suitable for nanopaper preparation using a simple vacuum filtration process.					
31954796	8	5	theme	crustacean	1397:1406	arg1	chitin					1408:1413	crustacean chitin	1397:1413	crustacean chitin	1397:1413	It was found that fungal extract nanopapers had distinct physico-chemical surface properties, being more hydrophobic than crustacean chitin.					
31954796	6	6	theme	extract	1003:1009	arg1	dispersion					1011:1020	the extract dispersion	999:1020	the extract dispersion	999:1020	These extracts were readily disintegrated into nanofibre dimensions by a low-energy mechanical blending, thus making the extract dispersion directly suitable for nanopaper preparation using a simple vacuum filtration process.					
31954796	5	7	theme	mushroom	744:751	arg1	walls					778:782	common mushroom (Agaricus bisporus) cell walls	737:782	common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex	737:879	Here, chitin-glucan nanofibrils were extracted from common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex.					
31954796	2	8	from	structure	322:330	arg1	contrast					335:342	contrast	335:342	contrast to nanocellulose	335:359	It has a native nano-fibrous structure in contrast to nanocellulose, for which further nanofibrillation is required.					
31954796	4	9	theme	water	575:579	arg1	treatment					581:589	water treatment	575:589	water treatment due to their distinct surface properties inherited from the composition of chitin and glucan	575:682	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	5	10	theme	common	737:742	arg1	mushroom					744:751	common mushroom	737:751	common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex	737:879	Here, chitin-glucan nanofibrils were extracted from common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex.					
31954796	1	11	theme	structural	75:84	arg1	component					86:94	The structural component	71:94	The structural component of fungal cell walls	71:115	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	5	12	theme	chitin-glucan	859:871	arg1	complex					873:879	the chitin-glucan complex	855:879	the chitin-glucan complex	855:879	Here, chitin-glucan nanofibrils were extracted from common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex.					
31954796	2	13	theme	further	372:378	arg1	nanofibrillation					380:395	further nanofibrillation	372:395	further nanofibrillation	372:395	It has a native nano-fibrous structure in contrast to nanocellulose, for which further nanofibrillation is required.					
31954796	1	14	theme	chitin	257:262	arg1	toughness					272:280	the toughness	268:280	the toughness of glucan	268:290	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	1	14	theme	chitin	257:262	arg1	strength					245:252	the strength	241:252	the strength of chitin	241:262	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	5	15	theme	cell	773:776	arg1	walls					778:782	common mushroom (Agaricus bisporus) cell walls	737:782	common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex	737:879	Here, chitin-glucan nanofibrils were extracted from common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex.					
31954796	5	16	theme	isolation	801:809	arg1	procedure					811:819	a mild isolation procedure	794:819	a mild isolation procedure to preserve the native quality of the chitin-glucan complex	794:879	Here, chitin-glucan nanofibrils were extracted from common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex.					
31954796	7	17	theme	chitin	1218:1223	arg1	nanopapers					1225:1234	chitin nanopapers	1218:1234	chitin nanopapers of crustacean (Cancer pagurus) origin	1218:1272	Chitin-glucan nanopaper morphology, mechanical, chemical, and surface properties were studied and compared to chitin nanopapers of crustacean (Cancer pagurus) origin.					
31954796	5	18	theme	mild	796:799	arg1	procedure					811:819	a mild isolation procedure	794:819	a mild isolation procedure to preserve the native quality of the chitin-glucan complex	794:879	Here, chitin-glucan nanofibrils were extracted from common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex.					
31954796	7	19	theme	Cancer	1251:1256	arg1	origin					1267:1272	crustacean (Cancer pagurus) origin	1239:1272	crustacean (Cancer pagurus) origin	1239:1272	Chitin-glucan nanopaper morphology, mechanical, chemical, and surface properties were studied and compared to chitin nanopapers of crustacean (Cancer pagurus) origin.					
31954796	7	20	theme	Chitin-glucan	1108:1120	arg1	morphology					1132:1141	Chitin-glucan nanopaper morphology	1108:1141	Chitin-glucan nanopaper morphology	1108:1141	Chitin-glucan nanopaper morphology, mechanical, chemical, and surface properties were studied and compared to chitin nanopapers of crustacean (Cancer pagurus) origin.					
31954796	4	21	theme	chitin	666:671	arg1	composition					651:661	the composition	647:661	the composition of chitin and glucan	647:682	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	0	22	theme	Surface	0:6	arg1	properties					8:17	Surface properties	0:17	Surface properties of chitin-glucan	0:34	Surface properties of chitin-glucan nanopapers from Agaricus bisporus.					
31954796	7	23	theme	nanopaper	1122:1130	arg1	morphology					1132:1141	Chitin-glucan nanopaper morphology	1108:1141	Chitin-glucan nanopaper morphology	1108:1141	Chitin-glucan nanopaper morphology, mechanical, chemical, and surface properties were studied and compared to chitin nanopapers of crustacean (Cancer pagurus) origin.					
31954796	1	24	theme	fungal	99:104	arg1	walls					111:115	fungal cell walls	99:115	fungal cell walls	99:115	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	1	25	theme	native	185:190	arg1	material					202:209	a native composite material	183:209	a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan	183:290	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	0	26	theme	chitin-glucan	22:34	arg1	properties					8:17	Surface properties	0:17	Surface properties of chitin-glucan	0:34	Surface properties of chitin-glucan nanopapers from Agaricus bisporus.					
31954796	1	27	theme	cell	106:109	arg1	walls					111:115	fungal cell walls	99:115	fungal cell walls	99:115	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	1	28	theme	composite	192:200	arg1	material					202:209	a native composite material	183:209	a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan	183:290	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	1	29	dep	comprises	117:125	arg1	bonded					148:153	bonded	148:153	bonded to glucan	148:163	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	1	30	theme	walls	111:115	arg1	component					86:94	The structural component	71:94	The structural component of fungal cell walls	71:115	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	4	31	theme	surface	613:619	arg1	properties					621:630	their distinct surface properties	598:630	their distinct surface properties inherited from the composition of chitin and glucan	598:682	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	4	32	from	films	539:543	arg1	applicable					515:524	applicable	515:524	applicable	515:524	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	6	33	theme	mechanical	966:975	arg1	blending					977:984	a low-energy mechanical blending	953:984	a low-energy mechanical blending	953:984	These extracts were readily disintegrated into nanofibre dimensions by a low-energy mechanical blending, thus making the extract dispersion directly suitable for nanopaper preparation using a simple vacuum filtration process.					
31954796	8	34	theme	extract	1300:1306	arg1	nanopapers					1308:1317	fungal extract nanopapers	1293:1317	fungal extract nanopapers	1293:1317	It was found that fungal extract nanopapers had distinct physico-chemical surface properties, being more hydrophobic than crustacean chitin.					
31954796	5	35	theme	complex	873:879	arg1	quality					844:850	the native quality	833:850	the native quality of the chitin-glucan complex	833:879	Here, chitin-glucan nanofibrils were extracted from common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex.					
31954796	5	36	theme	native	837:842	arg1	quality					844:850	the native quality	833:850	the native quality of the chitin-glucan complex	833:879	Here, chitin-glucan nanofibrils were extracted from common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex.					
31954796	6	37	theme	filtration	1088:1097	arg1	process					1099:1105	a simple vacuum filtration process	1072:1105	a simple vacuum filtration process	1072:1105	These extracts were readily disintegrated into nanofibre dimensions by a low-energy mechanical blending, thus making the extract dispersion directly suitable for nanopaper preparation using a simple vacuum filtration process.					
31954796	1	38	theme	glucan	285:290	arg1	toughness					272:280	the toughness	268:280	the toughness of glucan	268:290	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	1	38	theme	glucan	285:290	arg1	strength					245:252	the strength	241:252	the strength of chitin	241:262	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	4	39	from	composites	546:555	arg1	applicable					515:524	applicable	515:524	applicable	515:524	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	2	40	contain	has	296:298	arg2	structure					322:330	a native nano-fibrous structure	300:330	a native nano-fibrous structure	300:330	It has a native nano-fibrous structure in contrast to nanocellulose, for which further nanofibrillation is required.					
31954796	2	40	contain	has	296:298	arg1	It					293:294	It	293:294	It	293:294	It has a native nano-fibrous structure in contrast to nanocellulose, for which further nanofibrillation is required.					
31954796	6	41	theme	low-energy	955:964	arg1	blending					977:984	a low-energy mechanical blending	953:984	a low-energy mechanical blending	953:984	These extracts were readily disintegrated into nanofibre dimensions by a low-energy mechanical blending, thus making the extract dispersion directly suitable for nanopaper preparation using a simple vacuum filtration process.					
31954796	4	42	from	applicable	515:524	arg1	composites					546:555	composites	546:555	composites	546:555	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	4	42	from	applicable	515:524	arg1	membranes					561:569	membranes	561:569	membranes	561:569	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	4	42	from	applicable	515:524	arg1	films					539:543	packaging films	529:543	packaging films	529:543	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	5	43	dep	mushroom	744:751	arg1	bisporus					763:770	bisporus	763:770	bisporus	763:770	Here, chitin-glucan nanofibrils were extracted from common mushroom (Agaricus bisporus) cell walls utilizing a mild isolation procedure to preserve the native quality of the chitin-glucan complex.					
31954796	2	44	theme	nano-fibrous	309:320	arg1	structure					322:330	a native nano-fibrous structure	300:330	a native nano-fibrous structure	300:330	It has a native nano-fibrous structure in contrast to nanocellulose, for which further nanofibrillation is required.					
31954796	4	45	theme	packaging	529:537	arg1	films					539:543	packaging films	529:543	packaging films	529:543	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	7	46	theme	surface	1170:1176	arg1	properties					1178:1187	surface properties	1170:1187	surface properties	1170:1187	Chitin-glucan nanopaper morphology, mechanical, chemical, and surface properties were studied and compared to chitin nanopapers of crustacean (Cancer pagurus) origin.					
31954796	2	47	theme	native	302:307	arg1	structure					322:330	a native nano-fibrous structure	300:330	a native nano-fibrous structure	300:330	It has a native nano-fibrous structure in contrast to nanocellulose, for which further nanofibrillation is required.					
31954796	6	48	theme	nanopaper	1044:1052	arg1	preparation					1054:1064	nanopaper preparation	1044:1064	nanopaper preparation using a simple vacuum filtration process	1044:1105	These extracts were readily disintegrated into nanofibre dimensions by a low-energy mechanical blending, thus making the extract dispersion directly suitable for nanopaper preparation using a simple vacuum filtration process.					
31954796	8	49	contain	had	1319:1321	arg1	nanopapers					1308:1317	fungal extract nanopapers	1293:1317	fungal extract nanopapers	1293:1317	It was found that fungal extract nanopapers had distinct physico-chemical surface properties, being more hydrophobic than crustacean chitin.					
31954796	8	49	contain	had	1319:1321	arg2	properties					1357:1366	distinct physico-chemical surface properties	1323:1366	distinct physico-chemical surface properties	1323:1366	It was found that fungal extract nanopapers had distinct physico-chemical surface properties, being more hydrophobic than crustacean chitin.					
31954796	6	50	theme	vacuum	1081:1086	arg1	process					1099:1105	a simple vacuum filtration process	1072:1105	a simple vacuum filtration process	1072:1105	These extracts were readily disintegrated into nanofibre dimensions by a low-energy mechanical blending, thus making the extract dispersion directly suitable for nanopaper preparation using a simple vacuum filtration process.					
31954796	8	51	theme	distinct	1323:1330	arg1	properties					1357:1366	distinct physico-chemical surface properties	1323:1366	distinct physico-chemical surface properties	1323:1366	It was found that fungal extract nanopapers had distinct physico-chemical surface properties, being more hydrophobic than crustacean chitin.					
31954796	8	52	theme	physico-chemical	1332:1347	arg1	properties					1357:1366	distinct physico-chemical surface properties	1323:1366	distinct physico-chemical surface properties	1323:1366	It was found that fungal extract nanopapers had distinct physico-chemical surface properties, being more hydrophobic than crustacean chitin.					
31954796	7	53	theme	pagurus	1258:1264	arg1	origin					1267:1272	crustacean (Cancer pagurus) origin	1239:1272	crustacean (Cancer pagurus) origin	1239:1272	Chitin-glucan nanopaper morphology, mechanical, chemical, and surface properties were studied and compared to chitin nanopapers of crustacean (Cancer pagurus) origin.					
31954796	3	54	theme	fungal	446:451	arg1	FChNFs					473:478	FChNFs	473:478	FChNFs	473:478	Nanopapers can be manufactured from fungal chitin nanofibrils (FChNFs).					
31954796	3	54	theme	fungal	446:451	arg1	nanofibrils					460:470	fungal chitin nanofibrils	446:470	fungal chitin nanofibrils (FChNFs)	446:479	Nanopapers can be manufactured from fungal chitin nanofibrils (FChNFs).					
31954796	8	55	theme	fungal	1293:1298	arg1	nanopapers					1308:1317	fungal extract nanopapers	1293:1317	fungal extract nanopapers	1293:1317	It was found that fungal extract nanopapers had distinct physico-chemical surface properties, being more hydrophobic than crustacean chitin.					
31954796	3	56	theme	chitin	453:458	arg1	FChNFs					473:478	FChNFs	473:478	FChNFs	473:478	Nanopapers can be manufactured from fungal chitin nanofibrils (FChNFs).					
31954796	3	56	theme	chitin	453:458	arg1	nanofibrils					460:470	fungal chitin nanofibrils	446:470	fungal chitin nanofibrils (FChNFs)	446:479	Nanopapers can be manufactured from fungal chitin nanofibrils (FChNFs).					
31954796	4	57	theme	glucan	677:682	arg1	composition					651:661	the composition	647:661	the composition of chitin and glucan	647:682	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	4	58	theme	distinct	604:611	arg1	properties					621:630	their distinct surface properties	598:630	their distinct surface properties inherited from the composition of chitin and glucan	598:682	FChNF nanopapers are potentially applicable in packaging films, composites, or membranes for water treatment due to their distinct surface properties inherited from the composition of chitin and glucan.					
31954796	6	59	theme	nanofibre	929:937	arg1	dimensions					939:948	nanofibre dimensions	929:948	nanofibre dimensions	929:948	These extracts were readily disintegrated into nanofibre dimensions by a low-energy mechanical blending, thus making the extract dispersion directly suitable for nanopaper preparation using a simple vacuum filtration process.					
31954796	1	60	dep	material	202:209	arg1	CG					227:228	CG	227:228	CG	227:228	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	1	60	dep	material	202:209	arg1	chitin-glucan					212:224	chitin-glucan	212:224	chitin-glucan	212:224	The structural component of fungal cell walls comprises of chitin covalently bonded to glucan; this constitutes a native composite material (chitin-glucan, CG) combining the strength of chitin and the toughness of glucan.					
31954796	8	61	theme	surface	1349:1355	arg1	properties					1357:1366	distinct physico-chemical surface properties	1323:1366	distinct physico-chemical surface properties	1323:1366	It was found that fungal extract nanopapers had distinct physico-chemical surface properties, being more hydrophobic than crustacean chitin.					
31954796	7	62	theme	crustacean	1239:1248	arg1	origin					1267:1272	crustacean (Cancer pagurus) origin	1239:1272	crustacean (Cancer pagurus) origin	1239:1272	Chitin-glucan nanopaper morphology, mechanical, chemical, and surface properties were studied and compared to chitin nanopapers of crustacean (Cancer pagurus) origin.					
32070507	4	0	dep	de	506:507	arg1	Gennes					509:514	Gennes	509:514	Gennes	509:514	We evaluate different methods for estimating the entanglement cross-over, following the de Gennes scaling and hydrodynamic scaling models, and show that these lead to similar results.					
32070507	4	1	dep	scaling	516:522	arg1	de					506:507	de	506:507	de	506:507	We evaluate different methods for estimating the entanglement cross-over, following the de Gennes scaling and hydrodynamic scaling models, and show that these lead to similar results.					
32070507	6	2	dep	long	963:966	arg1	as					968:969	as	968:969	as	968:969	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
32070507	5	3	theme	entanglement	612:623	arg1	concentration					625:637	the entanglement concentration	608:637	the entanglement concentration	608:637	Above the entanglement concentration, the specific viscosity, longest relaxation time and plateau modulus scale as ηsp ≃ N3.9c4.2, τ ≃ N3.9c2.4 and GP ≃ N0c1.9.					
32070507	1	4	theme	semiflexible	142:153	arg1	polymer					155:161	a semiflexible polymer	140:161	a semiflexible polymer	140:161	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	1	4	theme	semiflexible	142:153	arg1	cellulose					123:131	hydroxypropyl cellulose	109:131	hydroxypropyl cellulose (HPC)	109:137	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	5	5	theme	plateau	692:698	arg1	modulus					700:706	plateau modulus	692:706	plateau modulus	692:706	Above the entanglement concentration, the specific viscosity, longest relaxation time and plateau modulus scale as ηsp ≃ N3.9c4.2, τ ≃ N3.9c2.4 and GP ≃ N0c1.9.					
32070507	2	6	theme	Kuhn	260:263	arg1	≃22 nm					272:277	a Kuhn length ≃22 nm	258:277	a Kuhn length ≃22 nm	258:277	Intrinsic viscosity data are consistent with a polymer in θ solvent with a Kuhn length ≃22 nm.					
32070507	3	7	with	scales	362:367	arg1	degree					378:383	the degree	374:383	the degree of polymerisation as c* ∝ N-0.9	374:415	The overlap concentration, estimated as the reciprocal of the intrinsic viscosity scales with the degree of polymerisation as c* ∝ N-0.9.					
32070507	6	8	theme	polysaccharide	873:886	arg1	systems					888:894	many polysaccharide systems	868:894	many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected	868:1020	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
32070507	6	9	theme	other	781:785	arg1	polymers					787:794	other polymers	781:794	other polymers	781:794	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
32070507	1	10	theme	dilute	56:61	arg1	properties					72:81	dilute solution properties	56:81	dilute solution properties	56:81	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	6	11	dep	varying	899:905	arg1	neglected					1012:1020	neglected	1012:1020	varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected	899:1020	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
32070507	4	12	theme	different	430:438	arg1	methods					440:446	different methods	430:446	different methods for estimating the entanglement cross-over, following the de Gennes scaling and hydrodynamic scaling models, and show that these lead to similar results	430:599	We evaluate different methods for estimating the entanglement cross-over, following the de Gennes scaling and hydrodynamic scaling models, and show that these lead to similar results.					
32070507	1	13	theme	solution	63:70	arg1	properties					72:81	dilute solution properties	56:81	dilute solution properties	56:81	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	3	14	theme	c	406:406	arg1	*					407:407	c* ∝ N-0.9	406:415	c* ∝ N-0.9	406:415	The overlap concentration, estimated as the reciprocal of the intrinsic viscosity scales with the degree of polymerisation as c* ∝ N-0.9.					
32070507	3	15	theme	viscosity	352:360	arg1	scales					362:367	the intrinsic viscosity scales	338:367	the intrinsic viscosity scales with the degree of polymerisation as c* ∝ N-0.9	338:415	The overlap concentration, estimated as the reciprocal of the intrinsic viscosity scales with the degree of polymerisation as c* ∝ N-0.9.					
32070507	4	16	theme	hydrodynamic	528:539	arg1	models					549:554	the de Gennes scaling and hydrodynamic scaling models	502:554	models	549:554	We evaluate different methods for estimating the entanglement cross-over, following the de Gennes scaling and hydrodynamic scaling models, and show that these lead to similar results.					
32070507	7	17	theme	observed	1046:1053	arg1	laws					1063:1066	the observed scaling laws	1042:1066	the observed scaling laws	1042:1066	On the other hand, the observed scaling laws differ appreciably from those of synthetic flexible polymers in good or θ-solvent.					
32070507	6	18	theme	many	868:871	arg1	systems					888:894	many polysaccharide systems	868:894	many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected	868:1020	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
32070507	3	19	theme	polymerisation	388:401	arg1	degree					378:383	the degree	374:383	the degree of polymerisation as c* ∝ N-0.9	374:415	The overlap concentration, estimated as the reciprocal of the intrinsic viscosity scales with the degree of polymerisation as c* ∝ N-0.9.					
32070507	3	20	theme	scales	362:367	arg1	reciprocal					324:333	reciprocal	324:333	reciprocal	324:333	The overlap concentration, estimated as the reciprocal of the intrinsic viscosity scales with the degree of polymerisation as c* ∝ N-0.9.					
32070507	7	21	theme	scaling	1055:1061	arg1	laws					1063:1066	the observed scaling laws	1042:1066	the observed scaling laws	1042:1066	On the other hand, the observed scaling laws differ appreciably from those of synthetic flexible polymers in good or θ-solvent.					
32070507	1	22	theme	entangled	87:95	arg1	dynamics					97:104	entangled dynamics	87:104	entangled dynamics	87:104	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	2	23	from	polymer	232:238	arg1	solvent					245:251	θ solvent	243:251	θ solvent	243:251	Intrinsic viscosity data are consistent with a polymer in θ solvent with a Kuhn length ≃22 nm.					
32070507	1	24	theme	aqueous	167:173	arg1	solution					175:182	aqueous solution	167:182	aqueous solution	167:182	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	0	25	theme	entangled	16:24	arg1	polysaccharides					26:40	entangled polysaccharides	16:40	entangled polysaccharides	16:40	Scaling laws of entangled polysaccharides.					
32070507	5	26	theme	longest	664:670	arg1	time					683:686	longest relaxation time	664:686	longest relaxation time	664:686	Above the entanglement concentration, the specific viscosity, longest relaxation time and plateau modulus scale as ηsp ≃ N3.9c4.2, τ ≃ N3.9c2.4 and GP ≃ N0c1.9.					
32070507	3	27	theme	intrinsic	342:350	arg1	scales					362:367	the intrinsic viscosity scales	338:367	the intrinsic viscosity scales with the degree of polymerisation as c* ∝ N-0.9	338:415	The overlap concentration, estimated as the reciprocal of the intrinsic viscosity scales with the degree of polymerisation as c* ∝ N-0.9.					
32070507	4	28	theme	scaling	541:547	arg1	models					549:554	the de Gennes scaling and hydrodynamic scaling models	502:554	models	549:554	We evaluate different methods for estimating the entanglement cross-over, following the de Gennes scaling and hydrodynamic scaling models, and show that these lead to similar results.					
32070507	5	29	theme	relaxation	672:681	arg1	time					683:686	longest relaxation time	664:686	longest relaxation time	664:686	Above the entanglement concentration, the specific viscosity, longest relaxation time and plateau modulus scale as ηsp ≃ N3.9c4.2, τ ≃ N3.9c2.4 and GP ≃ N0c1.9.					
32070507	2	30	theme	length	265:270	arg1	≃22 nm					272:277	a Kuhn length ≃22 nm	258:277	a Kuhn length ≃22 nm	258:277	Intrinsic viscosity data are consistent with a polymer in θ solvent with a Kuhn length ≃22 nm.					
32070507	4	31	theme	similar	585:591	arg1	results					593:599	similar results	585:599	similar results	585:599	We evaluate different methods for estimating the entanglement cross-over, following the de Gennes scaling and hydrodynamic scaling models, and show that these lead to similar results.					
32070507	2	32	with	consistent	214:223	arg1	polymer					232:238	a polymer	230:238	a polymer in θ solvent with a Kuhn length ≃22 nm	230:277	Intrinsic viscosity data are consistent with a polymer in θ solvent with a Kuhn length ≃22 nm.					
32070507	7	33	theme	other	1030:1034	arg1	hand					1036:1039	the other hand	1026:1039	the other hand	1026:1039	On the other hand, the observed scaling laws differ appreciably from those of synthetic flexible polymers in good or θ-solvent.					
32070507	6	34	theme	solvent	943:949	arg1	quality					951:957	solvent quality	943:957	solvent quality	943:957	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
32070507	0	35	theme	polysaccharides	26:40	arg1	laws					8:11	laws	8:11	laws of entangled polysaccharides	8:40	Scaling laws of entangled polysaccharides.					
32070507	2	36	with	polymer	232:238	arg1	≃22 nm					272:277	a Kuhn length ≃22 nm	258:277	a Kuhn length ≃22 nm	258:277	Intrinsic viscosity data are consistent with a polymer in θ solvent with a Kuhn length ≃22 nm.					
32070507	6	37	with	comparison	765:774	arg1	polymers					787:794	other polymers	781:794	other polymers	781:794	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
32070507	1	38	theme	hydroxypropyl	109:121	arg1	polymer					155:161	a semiflexible polymer	140:161	a semiflexible polymer	140:161	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	1	38	theme	hydroxypropyl	109:121	arg1	HPC					134:136	HPC	134:136	HPC	134:136	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	1	38	theme	hydroxypropyl	109:121	arg1	cellulose					123:131	hydroxypropyl cellulose	109:131	hydroxypropyl cellulose (HPC)	109:137	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	7	39	theme	flexible	1111:1118	arg1	polymers					1120:1127	synthetic flexible polymers	1101:1127	synthetic flexible polymers	1101:1127	On the other hand, the observed scaling laws differ appreciably from those of synthetic flexible polymers in good or θ-solvent.					
32070507	5	40	theme	specific	644:651	arg1	viscosity					653:661	the specific viscosity	640:661	the specific viscosity	640:661	Above the entanglement concentration, the specific viscosity, longest relaxation time and plateau modulus scale as ηsp ≃ N3.9c4.2, τ ≃ N3.9c2.4 and GP ≃ N0c1.9.					
32070507	2	41	theme	viscosity	195:203	arg1	data					205:208	Intrinsic viscosity data	185:208	Intrinsic viscosity data	185:208	Intrinsic viscosity data are consistent with a polymer in θ solvent with a Kuhn length ≃22 nm.					
32070507	6	42	theme	rheological	814:824	arg1	common					858:863	common	858:863	common	858:863	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
32070507	6	42	theme	rheological	814:824	arg1	properties					826:835	the rheological properties	810:835	the rheological properties displayed by HPC	810:852	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
32070507	1	43	theme	cellulose	123:131	arg1	properties					72:81	dilute solution properties	56:81	dilute solution properties	56:81	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	1	43	theme	cellulose	123:131	arg1	dynamics					97:104	entangled dynamics	87:104	entangled dynamics	87:104	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	2	44	theme	Intrinsic	185:193	arg1	data					205:208	Intrinsic viscosity data	185:208	Intrinsic viscosity data	185:208	Intrinsic viscosity data are consistent with a polymer in θ solvent with a Kuhn length ≃22 nm.					
32070507	2	45	theme	θ	243:243	arg1	solvent					245:251	θ solvent	243:251	θ solvent	243:251	Intrinsic viscosity data are consistent with a polymer in θ solvent with a Kuhn length ≃22 nm.					
32070507	1	46	dep	properties	72:81	arg1	the					52:54	the	52:54	the	52:54	We study the dilute solution properties and entangled dynamics of hydroxypropyl cellulose (HPC), a semiflexible polymer, in aqueous solution.					
32070507	6	47	theme	hyper-entanglements	985:1003	arg1	effect					975:980	the effect	971:980	the effect of hyper-entanglements	971:1003	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
32070507	7	48	theme	synthetic	1101:1109	arg1	polymers					1120:1127	synthetic flexible polymers	1101:1127	synthetic flexible polymers	1101:1127	On the other hand, the observed scaling laws differ appreciably from those of synthetic flexible polymers in good or θ-solvent.					
32070507	6	49	theme	backbone	907:914	arg1	composition					916:926	backbone composition	907:926	backbone composition	907:926	A comparison with other polymers suggests that the rheological properties displayed by HPC are common to many polysaccharide systems of varying backbone composition, stiffness and solvent quality, as long as the effect of hyper-entanglements can be neglected.					
34973721	2	0	theme	RG-I	513:516	arg1	WGBP-A2b					539:546	WGBP-A2b	539:546	WGBP-A2b	539:546	We isolated acidic polysaccharides (WGBP-A2) and purified a RG-I type polysaccharide (WGBP-A2b) with a molecular weight of 44 kDa.					
34973721	2	0	theme	RG-I	513:516	arg1	polysaccharide					523:536	a RG-I type polysaccharide	511:536	purified a RG-I type polysaccharide (WGBP-A2b)	502:547	We isolated acidic polysaccharides (WGBP-A2) and purified a RG-I type polysaccharide (WGBP-A2b) with a molecular weight of 44 kDa.					
34973721	3	1	theme	Inflammatory	739:750	arg1	factors					752:758	Inflammatory factors	739:758	Inflammatory factors	739:758	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
34973721	1	2	theme	purification	152:163	arg1	technology					165:174	the separation and purification technology	133:174	technology	165:174	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	1	3	theme	composition	250:260	arg1	characteristics					262:276	its composition characteristics	246:276	its composition characteristics	246:276	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	4	4	from	HUVECs	851:856	arg1	expressions					799:809	the expressions	795:809	the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b	795:878	On a cellular level, the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b were down-regulated.					
34973721	3	5	theme	alopecia	692:699	arg1	areata					701:706	alopecia areata	692:706	alopecia areata mice	692:711	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
34973721	1	6	theme	polysaccharide	357:370	arg1	fraction					372:379	the polysaccharide fraction	353:379	the polysaccharide fraction with bioactive activities	353:405	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	6	7	from	expression	1062:1071	arg1	pathway					1145:1151	the inflammation signaling pathway	1118:1151	the inflammation signaling pathway	1118:1151	Its specific mechanism may be related to its regulating the expression of p-p65 p-IκBα, TNF-α and IL-1β proteins in the inflammation signaling pathway.					
34973721	5	8	theme	signaling	957:965	arg1	pathway					967:973	the inflammation signaling pathway	940:973	the inflammation signaling pathway	940:973	The bioinformatic analysis showed that the inflammation signaling pathway was significantly changed.					
34973721	5	9	theme	inflammation	944:955	arg1	pathway					967:973	the inflammation signaling pathway	940:973	the inflammation signaling pathway	940:973	The bioinformatic analysis showed that the inflammation signaling pathway was significantly changed.					
34973721	6	10	theme	TNF-α	1090:1094	arg1	proteins					1106:1113	p-p65 p-IκBα, TNF-α and IL-1β proteins	1076:1113	p-p65 p-IκBα, TNF-α and IL-1β proteins	1076:1113	Its specific mechanism may be related to its regulating the expression of p-p65 p-IκBα, TNF-α and IL-1β proteins in the inflammation signaling pathway.					
34973721	4	11	from	TNF-α	832:836	arg1	HUVECs					851:856	HUVECs	851:856	HUVECs treated with WGBP-A2b	851:878	On a cellular level, the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b were down-regulated.					
34973721	6	12	theme	p-IκBα	1082:1087	arg1	proteins					1106:1113	p-p65 p-IκBα, TNF-α and IL-1β proteins	1076:1113	p-p65 p-IκBα, TNF-α and IL-1β proteins	1076:1113	Its specific mechanism may be related to its regulating the expression of p-p65 p-IκBα, TNF-α and IL-1β proteins in the inflammation signaling pathway.					
34973721	4	13	theme	p-IκBα	824:829	arg1	expressions					799:809	the expressions	795:809	the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b	795:878	On a cellular level, the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b were down-regulated.					
34973721	1	14	theme	bioactive	386:394	arg1	activities					396:405	bioactive activities	386:405	bioactive activities	386:405	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	1	15	from	effect	313:318	arg1	mice					339:342	alopecia areata mice	323:342	alopecia areata mice	323:342	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	3	16	from	contents	645:652	arg1	tissue					682:687	the skin tissue	673:687	the skin tissue of alopecia areata mice	673:711	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
34973721	1	17	theme	water-soluble	179:191	arg1	biloba					200:205	water-soluble Ginkgo biloba	179:205	water-soluble Ginkgo biloba leaves polysaccharides (WGBP)	179:235	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	4	18	from	p-IκBα	824:829	arg1	HUVECs					851:856	HUVECs	851:856	HUVECs treated with WGBP-A2b	851:878	On a cellular level, the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b were down-regulated.					
34973721	1	19	theme	Ginkgo	193:198	arg1	biloba					200:205	water-soluble Ginkgo biloba	179:205	water-soluble Ginkgo biloba leaves polysaccharides (WGBP)	179:235	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	6	20	theme	specific	1006:1013	arg1	mechanism					1015:1023	Its specific mechanism	1002:1023	Its specific mechanism	1002:1023	Its specific mechanism may be related to its regulating the expression of p-p65 p-IκBα, TNF-α and IL-1β proteins in the inflammation signaling pathway.					
34973721	1	21	theme	biloba	200:205	arg1	technology					165:174	the separation and purification technology	133:174	technology	165:174	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	2	22	theme	acidic	465:470	arg1	polysaccharides					472:486	acidic polysaccharides	465:486	acidic polysaccharides (WGBP-A2)	465:496	We isolated acidic polysaccharides (WGBP-A2) and purified a RG-I type polysaccharide (WGBP-A2b) with a molecular weight of 44 kDa.					
34973721	2	22	theme	acidic	465:470	arg1	WGBP-A2					489:495	WGBP-A2	489:495	WGBP-A2	489:495	We isolated acidic polysaccharides (WGBP-A2) and purified a RG-I type polysaccharide (WGBP-A2b) with a molecular weight of 44 kDa.					
34973721	1	23	theme	hair-growth	291:301	arg1	effect					313:318	its hair-growth promoting effect	287:318	its hair-growth promoting effect in alopecia areata mice	287:342	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	6	24	theme	signaling	1135:1143	arg1	pathway					1145:1151	the inflammation signaling pathway	1118:1151	the inflammation signaling pathway	1118:1151	Its specific mechanism may be related to its regulating the expression of p-p65 p-IκBα, TNF-α and IL-1β proteins in the inflammation signaling pathway.					
34973721	3	25	dep	increase	632:639	arg1	decrease					714:721	decrease	714:721	decrease the contents of Inflammatory factors in the serum	714:771	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
34973721	1	26	theme	promoting	303:311	arg1	effect					313:318	its hair-growth promoting effect	287:318	its hair-growth promoting effect in alopecia areata mice	287:342	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	3	27	theme	HGF	666:668	arg1	contents					645:652	the contents	641:652	the contents of VEGF and HGF in the skin tissue of alopecia areata mice	641:711	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
34973721	1	28	theme	study	111:115	arg1	aim					99:101	The aim	95:101	The aim of this study	95:115	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	6	29	theme	IL-1β	1100:1104	arg1	proteins					1106:1113	p-p65 p-IκBα, TNF-α and IL-1β proteins	1076:1113	p-p65 p-IκBα, TNF-α and IL-1β proteins	1076:1113	Its specific mechanism may be related to its regulating the expression of p-p65 p-IκBα, TNF-α and IL-1β proteins in the inflammation signaling pathway.					
34973721	2	30	theme	purified	502:509	arg1	WGBP-A2b					539:546	WGBP-A2b	539:546	WGBP-A2b	539:546	We isolated acidic polysaccharides (WGBP-A2) and purified a RG-I type polysaccharide (WGBP-A2b) with a molecular weight of 44 kDa.					
34973721	2	30	theme	purified	502:509	arg1	polysaccharide					523:536	a RG-I type polysaccharide	511:536	purified a RG-I type polysaccharide (WGBP-A2b)	502:547	We isolated acidic polysaccharides (WGBP-A2) and purified a RG-I type polysaccharide (WGBP-A2b) with a molecular weight of 44 kDa.					
34973721	3	31	theme	factors	752:758	arg1	contents					727:734	the contents	723:734	the contents of Inflammatory factors in the serum	723:771	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
34973721	2	32	theme	molecular	556:564	arg1	weight					566:571	a molecular weight	554:571	a molecular weight of 44 kDa	554:581	We isolated acidic polysaccharides (WGBP-A2) and purified a RG-I type polysaccharide (WGBP-A2b) with a molecular weight of 44 kDa.					
34973721	0	33	theme	anti-inflammatory	33:49	arg1	mechanism					51:59	anti-inflammatory mechanism	33:59	anti-inflammatory mechanism of Ginkgo biloba polysaccharides	33:92	Hair-growth promoting effect and anti-inflammatory mechanism of Ginkgo biloba polysaccharides.					
34973721	3	34	theme	VEGF	657:660	arg1	contents					645:652	the contents	641:652	the contents of VEGF and HGF in the skin tissue of alopecia areata mice	641:711	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
34973721	1	35	dep	biloba	200:205	arg1	leaves					207:212	leaves	207:212	leaves polysaccharides (WGBP)	207:235	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	0	36	theme	Ginkgo	64:69	arg1	polysaccharides					78:92	Ginkgo biloba polysaccharides	64:92	Ginkgo biloba polysaccharides	64:92	Hair-growth promoting effect and anti-inflammatory mechanism of Ginkgo biloba polysaccharides.					
34973721	1	37	theme	alopecia	323:330	arg1	mice					339:342	alopecia areata mice	323:342	alopecia areata mice	323:342	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	2	38	theme	type	518:521	arg1	WGBP-A2b					539:546	WGBP-A2b	539:546	WGBP-A2b	539:546	We isolated acidic polysaccharides (WGBP-A2) and purified a RG-I type polysaccharide (WGBP-A2b) with a molecular weight of 44 kDa.					
34973721	2	38	theme	type	518:521	arg1	polysaccharide					523:536	a RG-I type polysaccharide	511:536	purified a RG-I type polysaccharide (WGBP-A2b)	502:547	We isolated acidic polysaccharides (WGBP-A2) and purified a RG-I type polysaccharide (WGBP-A2b) with a molecular weight of 44 kDa.					
34973721	4	39	from	p-p65	814:818	arg1	HUVECs					851:856	HUVECs	851:856	HUVECs treated with WGBP-A2b	851:878	On a cellular level, the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b were down-regulated.					
34973721	6	40	theme	inflammation	1122:1133	arg1	pathway					1145:1151	the inflammation signaling pathway	1118:1151	the inflammation signaling pathway	1118:1151	Its specific mechanism may be related to its regulating the expression of p-p65 p-IκBα, TNF-α and IL-1β proteins in the inflammation signaling pathway.					
34973721	3	41	theme	areata	701:706	arg1	mice					708:711	alopecia areata mice	692:711	alopecia areata mice	692:711	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
34973721	1	42	theme	areata	332:337	arg1	mice					339:342	alopecia areata mice	323:342	alopecia areata mice	323:342	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	1	43	theme	anti-inflammation	424:440	arg1	mechanism					442:450	its anti-inflammation mechanism	420:450	its anti-inflammation mechanism	420:450	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	4	44	theme	p-p65	814:818	arg1	expressions					799:809	the expressions	795:809	the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b	795:878	On a cellular level, the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b were down-regulated.					
34973721	4	45	from	expressions	799:809	arg1	HUVECs					851:856	HUVECs	851:856	HUVECs treated with WGBP-A2b	851:878	On a cellular level, the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b were down-regulated.					
34973721	5	46	theme	bioinformatic	905:917	arg1	analysis					919:926	The bioinformatic analysis	901:926	The bioinformatic analysis	901:926	The bioinformatic analysis showed that the inflammation signaling pathway was significantly changed.					
34973721	2	47	theme	44 kDa	576:581	arg1	weight					566:571	a molecular weight	554:571	a molecular weight of 44 kDa	554:581	We isolated acidic polysaccharides (WGBP-A2) and purified a RG-I type polysaccharide (WGBP-A2b) with a molecular weight of 44 kDa.					
34973721	4	48	theme	cellular	779:786	arg1	level					788:792	a cellular level	777:792	a cellular level	777:792	On a cellular level, the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b were down-regulated.					
34973721	6	49	theme	proteins	1106:1113	arg1	expression					1062:1071	the expression	1058:1071	the expression of p-p65 p-IκBα, TNF-α and IL-1β proteins in the inflammation signaling pathway	1058:1151	Its specific mechanism may be related to its regulating the expression of p-p65 p-IκBα, TNF-α and IL-1β proteins in the inflammation signaling pathway.					
34973721	1	50	with	fraction	372:379	arg1	activities					396:405	bioactive activities	386:405	bioactive activities	386:405	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	0	51	theme	polysaccharides	78:92	arg1	effect					22:27	effect	22:27	effect	22:27	Hair-growth promoting effect and anti-inflammatory mechanism of Ginkgo biloba polysaccharides.					
34973721	0	51	theme	polysaccharides	78:92	arg1	mechanism					51:59	anti-inflammatory mechanism	33:59	anti-inflammatory mechanism of Ginkgo biloba polysaccharides	33:92	Hair-growth promoting effect and anti-inflammatory mechanism of Ginkgo biloba polysaccharides.					
34973721	3	52	theme	skin	677:680	arg1	tissue					682:687	the skin tissue	673:687	the skin tissue of alopecia areata mice	673:711	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
34973721	4	53	from	IL-1β	842:846	arg1	HUVECs					851:856	HUVECs	851:856	HUVECs treated with WGBP-A2b	851:878	On a cellular level, the expressions of p-p65 and p-IκBα, TNF-α and IL-1β in HUVECs treated with WGBP-A2b were down-regulated.					
34973721	1	54	theme	separation	137:146	arg1	technology					165:174	the separation and purification technology	133:174	technology	165:174	The aim of this study was to optimize the separation and purification technology of water-soluble Ginkgo biloba leaves polysaccharides (WGBP), analyze its composition characteristics, observe its hair-growth promoting effect in alopecia areata mice, clarify the polysaccharide fraction with bioactive activities, and explore its anti-inflammation mechanism.					
34973721	0	55	theme	biloba	71:76	arg1	polysaccharides					78:92	Ginkgo biloba polysaccharides	64:92	Ginkgo biloba polysaccharides	64:92	Hair-growth promoting effect and anti-inflammatory mechanism of Ginkgo biloba polysaccharides.					
34973721	3	56	theme	mice	708:711	arg1	tissue					682:687	the skin tissue	673:687	the skin tissue of alopecia areata mice	673:711	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
34973721	3	57	from	contents	727:734	arg1	serum					767:771	the serum	763:771	the serum	763:771	Results showed that WGBP-A2 could significantly increase the contents of VEGF and HGF in the skin tissue of alopecia areata mice, decrease the contents of Inflammatory factors in the serum.					
32574745	1	0	from	structure	158:166	arg1	militaris					229:237	Cordyceps militaris	219:237	Cordyceps militaris cultivated with different initial growth pH	219:281	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	6	1	theme	ontology	832:839	arg1	terms					841:845	oxidative phosphorylation-related Gene ontology terms	793:845	oxidative phosphorylation-related Gene ontology terms	793:845	Furthermore, oxidative phosphorylation-related Gene ontology terms were up-regulated in CMsB groups.					
32574745	7	2	contain	possessed	1066:1074	arg2	content					1100:1106	higher β-(1 → 6)-glucan content	1076:1106	higher β-(1 → 6)-glucan content	1076:1106	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	7	2	contain	possessed	1066:1074	arg1	polysaccharides					1050:1064	C. militaris polysaccharides	1037:1064	C. militaris polysaccharides	1037:1064	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	7	2	contain	possessed	1066:1074	arg2	activities					1124:1133	antioxidant activities	1112:1133	antioxidant activities	1112:1133	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	1	3	theme	different	255:263	arg1	pH					280:281	different initial growth pH	255:281	different initial growth pH	255:281	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	6	4	theme	Gene	827:830	arg1	terms					841:845	oxidative phosphorylation-related Gene ontology terms	793:845	oxidative phosphorylation-related Gene ontology terms	793:845	Furthermore, oxidative phosphorylation-related Gene ontology terms were up-regulated in CMsB groups.					
32574745	7	5	theme	C.	1037:1038	arg1	polysaccharides					1050:1064	C. militaris polysaccharides	1037:1064	C. militaris polysaccharides	1037:1064	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	3	6	theme	initial	509:515	arg1	pH					524:525	an initial growth pH	506:525	an initial growth pH of 8-9 (CMsB)	506:539	Based on the results, C. militaris growing at an initial growth pH of 5-7 (CMsA) was distinguished from C. militaris growing at an initial growth pH of 8-9 (CMsB) and their unigenes showed the comparable expression.					
32574745	4	7	theme	β-glucan	606:613	arg1	content					615:621	β-glucan content	606:621	β-glucan content of CMsB group	606:635	The mean of β-glucan content of CMsB group was 32.7% (w/w), 10% higher than that of CMsA.					
32574745	1	8	theme	initial	265:271	arg1	pH					280:281	different initial growth pH	255:281	different initial growth pH	255:281	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	0	9	theme	antioxidant	88:98	arg1	activity					100:107	antioxidant activity	88:107	antioxidant activity	88:107	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	0	9	theme	antioxidant	88:98	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.	0:108	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	6	10	theme	phosphorylation-related	803:825	arg1	terms					841:845	oxidative phosphorylation-related Gene ontology terms	793:845	oxidative phosphorylation-related Gene ontology terms	793:845	Furthermore, oxidative phosphorylation-related Gene ontology terms were up-regulated in CMsB groups.					
32574745	7	11	theme	militaris	1040:1048	arg1	polysaccharides					1050:1064	C. militaris polysaccharides	1037:1064	C. militaris polysaccharides	1037:1064	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	3	12	theme	growth	517:522	arg1	pH					524:525	an initial growth pH	506:525	an initial growth pH of 8-9 (CMsB)	506:539	Based on the results, C. militaris growing at an initial growth pH of 5-7 (CMsA) was distinguished from C. militaris growing at an initial growth pH of 8-9 (CMsB) and their unigenes showed the comparable expression.					
32574745	7	13	dep	analysis	920:927	arg1	composition					960:970	monosaccharide composition	945:970	monosaccharide composition	945:970	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	7	13	dep	analysis	920:927	arg1	oxidation					983:991	periodate oxidation	973:991	periodate oxidation	973:991	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	7	13	dep	analysis	920:927	arg1	spectrum					935:942	FTIR spectrum	930:942	FTIR spectrum	930:942	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	1	14	theme	growth	273:278	arg1	pH					280:281	different initial growth pH	255:281	different initial growth pH	255:281	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	7	15	theme	antioxidant	1112:1122	arg1	activities					1124:1133	antioxidant activities	1112:1133	antioxidant activities	1112:1133	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	6	16	theme	oxidative	793:801	arg1	terms					841:845	oxidative phosphorylation-related Gene ontology terms	793:845	oxidative phosphorylation-related Gene ontology terms	793:845	Furthermore, oxidative phosphorylation-related Gene ontology terms were up-regulated in CMsB groups.					
32574745	7	17	theme	-glucan	1092:1098	arg1	content					1100:1106	higher β-(1 → 6)-glucan content	1076:1106	higher β-(1 → 6)-glucan content	1076:1106	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	1	18	from	content	137:143	arg1	militaris					229:237	Cordyceps militaris	219:237	Cordyceps militaris cultivated with different initial growth pH	219:281	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	1	19	theme	primary	150:156	arg1	structure					158:166	the primary structure	146:166	the primary structure	146:166	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	7	20	theme	β-	1083:1084	arg1	content					1100:1106	higher β-(1 → 6)-glucan content	1076:1106	higher β-(1 → 6)-glucan content	1076:1106	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	5	21	theme	expressed	734:742	arg1	genes					744:748	1088 differentially expressed genes	714:748	1088 differentially expressed genes	714:748	The results of RNA-seq showed 1088 differentially expressed genes between CMsA and CMsB groups.					
32574745	7	22	theme	structural	909:918	arg1	analysis					920:927	structural analysis	909:927	structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation)	909:992	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	3	23	theme	growth	435:440	arg1	pH					442:443	an initial growth pH	424:443	an initial growth pH of 5-7 (CMsA)	424:457	Based on the results, C. militaris growing at an initial growth pH of 5-7 (CMsA) was distinguished from C. militaris growing at an initial growth pH of 8-9 (CMsB) and their unigenes showed the comparable expression.					
32574745	5	24	theme	RNA-seq	699:705	arg1	results					688:694	The results	684:694	The results of RNA-seq	684:705	The results of RNA-seq showed 1088 differentially expressed genes between CMsA and CMsB groups.					
32574745	7	25	theme	bioactivity	998:1008	arg1	evaluation					1010:1019	bioactivity evaluation	998:1019	bioactivity evaluation	998:1019	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	3	26	theme	8-9	530:532	arg1	pH					524:525	an initial growth pH	506:525	an initial growth pH of 8-9 (CMsB)	506:539	Based on the results, C. militaris growing at an initial growth pH of 5-7 (CMsA) was distinguished from C. militaris growing at an initial growth pH of 8-9 (CMsB) and their unigenes showed the comparable expression.					
32574745	7	27	theme	evaluation	1010:1019	arg1	results					898:904	the results	894:904	the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation	894:1019	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	4	28	theme	content	615:621	arg1	mean					598:601	The mean	594:601	The mean of β-glucan content of CMsB group	594:635	The mean of β-glucan content of CMsB group was 32.7% (w/w), 10% higher than that of CMsA.					
32574745	4	28	theme	content	615:621	arg1	%					645:645	32.7%	641:645	32.7% (w/w)	641:651	The mean of β-glucan content of CMsB group was 32.7% (w/w), 10% higher than that of CMsA.					
32574745	4	29	theme	higher	658:663	arg1	%					656:656	10%	654:656	10% higher than that of CMsA	654:681	The mean of β-glucan content of CMsB group was 32.7% (w/w), 10% higher than that of CMsA.					
32574745	4	29	theme	higher	658:663	arg1	%					645:645	32.7%	641:645	32.7% (w/w)	641:651	The mean of β-glucan content of CMsB group was 32.7% (w/w), 10% higher than that of CMsA.					
32574745	1	30	theme	antioxidant	176:186	arg1	capacity					188:195	the antioxidant capacity	172:195	the antioxidant capacity	172:195	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	0	31	theme	Cordyceps	21:29	arg1	miltaris					31:38	Cordyceps miltaris	21:38	Cordyceps miltaris cultured at different pH	21:63	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	4	32	theme	group	631:635	arg1	content					615:621	β-glucan content	606:621	β-glucan content of CMsB group	606:635	The mean of β-glucan content of CMsB group was 32.7% (w/w), 10% higher than that of CMsA.					
32574745	7	33	theme	periodate	973:981	arg1	oxidation					983:991	periodate oxidation	973:991	periodate oxidation	973:991	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	7	33	theme	periodate	973:981	arg1	spectrum					935:942	FTIR spectrum	930:942	FTIR spectrum	930:942	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	7	34	dep	content	1100:1106	arg1	1 → 6					1086:1090	1 → 6	1086:1090	1 → 6	1086:1090	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	3	35	theme	initial	427:433	arg1	pH					442:443	an initial growth pH	424:443	an initial growth pH of 5-7 (CMsA)	424:457	Based on the results, C. militaris growing at an initial growth pH of 5-7 (CMsA) was distinguished from C. militaris growing at an initial growth pH of 8-9 (CMsB) and their unigenes showed the comparable expression.					
32574745	7	36	theme	FTIR	930:933	arg1	composition					960:970	monosaccharide composition	945:970	monosaccharide composition	945:970	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	7	36	theme	FTIR	930:933	arg1	oxidation					983:991	periodate oxidation	973:991	periodate oxidation	973:991	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	7	36	theme	FTIR	930:933	arg1	spectrum					935:942	FTIR spectrum	930:942	FTIR spectrum	930:942	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	1	37	theme	polysaccharides	200:214	arg1	content					137:143	the β-glucan content	124:143	the β-glucan content	124:143	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	1	37	theme	polysaccharides	200:214	arg1	structure					158:166	the primary structure	146:166	the primary structure	146:166	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	1	37	theme	polysaccharides	200:214	arg1	capacity					188:195	the antioxidant capacity	172:195	the antioxidant capacity	172:195	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	7	38	theme	higher	1076:1081	arg1	content					1100:1106	higher β-(1 → 6)-glucan content	1076:1106	higher β-(1 → 6)-glucan content	1076:1106	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	6	39	theme	CMsB	868:871	arg1	groups					873:878	CMsB groups	868:878	CMsB groups	868:878	Furthermore, oxidative phosphorylation-related Gene ontology terms were up-regulated in CMsB groups.					
32574745	0	40	theme	different	52:60	arg1	pH					62:63	different pH	52:63	different pH	52:63	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	7	41	theme	analysis	920:927	arg1	results					898:904	the results	894:904	the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation	894:1019	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	2	42	theme	biosynthesis	336:347	arg1	Meanwhile					299:307	Meanwhile	299:307	Meanwhile	299:307	Meanwhile, the mechanism of β-glucan biosynthesis was investigated by RNA-Seq.					
32574745	2	42	theme	biosynthesis	336:347	arg1	mechanism					314:322	the mechanism	310:322	the mechanism of β-glucan biosynthesis	310:347	Meanwhile, the mechanism of β-glucan biosynthesis was investigated by RNA-Seq.					
32574745	1	43	from	capacity	188:195	arg1	militaris					229:237	Cordyceps militaris	219:237	Cordyceps militaris cultivated with different initial growth pH	219:281	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	2	44	theme	β-glucan	327:334	arg1	biosynthesis					336:347	β-glucan biosynthesis	327:347	β-glucan biosynthesis	327:347	Meanwhile, the mechanism of β-glucan biosynthesis was investigated by RNA-Seq.					
32574745	7	45	theme	monosaccharide	945:958	arg1	composition					960:970	monosaccharide composition	945:970	monosaccharide composition	945:970	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	7	45	theme	monosaccharide	945:958	arg1	spectrum					935:942	FTIR spectrum	930:942	FTIR spectrum	930:942	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
32574745	3	46	theme	comparable	571:580	arg1	expression					582:591	the comparable expression	567:591	the comparable expression	567:591	Based on the results, C. militaris growing at an initial growth pH of 5-7 (CMsA) was distinguished from C. militaris growing at an initial growth pH of 8-9 (CMsB) and their unigenes showed the comparable expression.					
32574745	1	47	theme	Cordyceps	219:227	arg1	militaris					229:237	Cordyceps militaris	219:237	Cordyceps militaris cultivated with different initial growth pH	219:281	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	0	48	theme	Sugar	66:70	arg1	composition					72:82	Sugar composition	66:82	Sugar composition	66:82	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	0	48	theme	Sugar	66:70	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.	0:108	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	5	49	theme	CMsA	758:761	arg1	groups					772:777	CMsA and CMsB groups	758:777	CMsA and CMsB groups	758:777	The results of RNA-seq showed 1088 differentially expressed genes between CMsA and CMsB groups.					
32574745	3	50	theme	5-7	448:450	arg1	pH					442:443	an initial growth pH	424:443	an initial growth pH of 5-7 (CMsA)	424:457	Based on the results, C. militaris growing at an initial growth pH of 5-7 (CMsA) was distinguished from C. militaris growing at an initial growth pH of 8-9 (CMsB) and their unigenes showed the comparable expression.					
32574745	1	51	theme	β-glucan	128:135	arg1	content					137:143	the β-glucan content	124:143	the β-glucan content	124:143	In the study, the β-glucan content, the primary structure and the antioxidant capacity of polysaccharides in Cordyceps militaris cultivated with different initial growth pH were evaluated.					
32574745	5	52	theme	CMsB	767:770	arg1	groups					772:777	CMsA and CMsB groups	758:777	CMsA and CMsB groups	758:777	The results of RNA-seq showed 1088 differentially expressed genes between CMsA and CMsB groups.					
32574745	0	53	from	miltaris	31:38	arg1	composition					72:82	Sugar composition	66:82	Sugar composition	66:82	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	0	53	from	miltaris	31:38	arg1	activity					100:107	antioxidant activity	88:107	antioxidant activity	88:107	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	0	53	from	miltaris	31:38	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.	0:108	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	0	54	dep	Polysaccharides	0:14	arg1	composition					72:82	Sugar composition	66:82	Sugar composition	66:82	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	0	54	dep	Polysaccharides	0:14	arg1	activity					100:107	antioxidant activity	88:107	antioxidant activity	88:107	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	0	54	dep	Polysaccharides	0:14	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.	0:108	Polysaccharides from Cordyceps miltaris cultured at different pH: Sugar composition and antioxidant activity.					
32574745	4	55	theme	CMsB	626:629	arg1	group					631:635	CMsB group	626:635	CMsB group	626:635	The mean of β-glucan content of CMsB group was 32.7% (w/w), 10% higher than that of CMsA.					
32574745	7	56	theme	CMsB	1138:1141	arg1	groups					1143:1148	CMsB groups	1138:1148	CMsB groups	1138:1148	In addition, the results of structural analysis (FTIR spectrum, monosaccharide composition, periodate oxidation) and bioactivity evaluation speculated that C. militaris polysaccharides possessed higher β-(1 → 6)-glucan content and antioxidant activities in CMsB groups.					
31948226	4	0	theme	fluid	1124:1128	arg1	precursor					1139:1147	a fluid hydrogel precursor	1122:1147	a fluid hydrogel precursor	1122:1147	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	3	1	theme	extrusion	745:753	arg1	printing					755:762	extrusion printing	745:762	extrusion printing	745:762	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	8	2	theme	solid	1982:1986	arg1	method					1954:1959	The proposed method	1941:1959	The proposed method	1941:1959	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	8	2	theme	solid	1982:1986	arg1	method					2005:2010	a solid object sculpting method	1980:2010	a solid object sculpting method	1980:2010	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	5	3	theme	cross-linked	1289:1300	arg1	microgels					1310:1318	gellan or enzymatically cross-linked gelatin microgels	1265:1318	gellan or enzymatically cross-linked gelatin microgels	1265:1318	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	9	4	theme	better	2287:2292	arg1	resolution					2303:2312	better printing resolution	2287:2312	better printing resolution	2287:2312	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	8	5	theme	sculpting	1995:2003	arg1	method					1954:1959	The proposed method	1941:1959	The proposed method	1941:1959	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	8	5	theme	sculpting	1995:2003	arg1	method					2005:2010	a solid object sculpting method	1980:2010	a solid object sculpting method	1980:2010	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	5	6	link	cross-linked	1289:1300	arg1	microgels					1310:1318	gellan or enzymatically cross-linked gelatin microgels	1265:1318	gellan or enzymatically cross-linked gelatin microgels	1265:1318	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	4	7	theme	continuous	1202:1211	arg1	phase					1213:1217	the continuous phase	1198:1217	the continuous phase of the composite hydrogel	1198:1243	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	3	8	theme	three-dimensional	774:790	arg1	features					792:799	three-dimensional features	774:799	three-dimensional features	774:799	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	8	9	theme	external	2022:2029	arg1	contours					2031:2038	external contours	2022:2038	external contours	2022:2038	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	4	10	theme	bath	990:993	arg1	material					995:1002	The composite matrix bath material	969:1002	The composite matrix bath material	969:1002	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	7	11	theme	solid	1900:1904	arg1	constructs					1906:1915	solid constructs	1900:1915	solid constructs	1900:1915	Cell-laden constructs with printed features are also evaluated; the microgel composite hydrogel matrices support cell activity, and printed channels enhance proliferation compared to solid constructs even in static culture.					
31948226	0	12	theme	Objects	126:132	arg1	Sculpting					107:115	Sculpting	107:115	Sculpting of Solid Objects	107:132	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	0	12	theme	Objects	126:132	arg1	Bioprinting					59:69	Embedded Bioprinting	50:69	Embedded Bioprinting of Perfusable Tissue Constructs	50:101	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	1	13	theme	fabrication	206:216	arg1	technologies					218:229	advanced fabrication technologies	197:229	advanced fabrication technologies	197:229	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	7	14	theme	printed	1744:1750	arg1	features					1752:1759	printed features	1744:1759	printed features	1744:1759	Cell-laden constructs with printed features are also evaluated; the microgel composite hydrogel matrices support cell activity, and printed channels enhance proliferation compared to solid constructs even in static culture.					
31948226	2	15	theme	composite	626:634	arg1	bath					643:646	a biocompatible microgel composite matrix bath	601:646	a biocompatible microgel composite matrix bath	601:646	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	2	16	theme	biocompatible	603:615	arg1	bath					643:646	a biocompatible microgel composite matrix bath	601:646	a biocompatible microgel composite matrix bath	601:646	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	1	17	theme	cell	426:429	arg1	compositions					431:442	spatially varying material and/or cell compositions	392:442	compositions	431:442	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	6	18	theme	embedded	1698:1705	arg1	printing					1707:1714	embedded printing	1698:1714	embedded printing	1698:1714	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	4	19	theme	composite	973:981	arg1	material					995:1002	The composite matrix bath material	969:1002	The composite matrix bath material	969:1002	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	9	20	used	utilized	2274:2281	arg2	materials					2257:2265	more advanced sacrificial materials	2231:2265	more advanced sacrificial materials	2231:2265	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	3	21	theme	sacrificial	859:869	arg1	material					871:878	the sacrificial material	855:878	the sacrificial material	855:878	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	8	22	theme	ink	2075:2077	arg1	shell					2054:2058	a shell	2052:2058	a shell of sacrificial ink	2052:2077	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	2	23	theme	custom	525:530	arg1	constructs					577:586	custom biomedical acellular and cell-laden hydrogel constructs	525:586	custom biomedical acellular and cell-laden hydrogel constructs	525:586	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	4	24	theme	composite	1226:1234	arg1	hydrogel					1236:1243	the composite hydrogel	1222:1243	the composite hydrogel	1222:1243	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	5	25	theme	gelatin	1302:1308	arg1	microgels					1310:1318	gellan or enzymatically cross-linked gelatin microgels	1265:1318	gellan or enzymatically cross-linked gelatin microgels	1265:1318	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	6	26	theme	composite	1670:1678	arg1	bath					1687:1690	the proposed composite matrix bath	1657:1690	the proposed composite matrix bath using embedded printing	1657:1714	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	3	27	theme	composite	942:950	arg1	matrix					961:966	the bulk composite hydrogel matrix	933:966	the bulk composite hydrogel matrix	933:966	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	9	28	used	used	2204:2207	arg2	solution					2192:2199	aqueous alginate solution	2175:2199	aqueous alginate solution	2175:2199	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	9	28	used	used	2204:2207	arg2	ink					2226:2228	a sacrificial ink	2212:2228	a sacrificial ink	2212:2228	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	5	29	theme	gelatin	1351:1357	arg1	solution					1378:1385	a continuous gelatin hydrogel precursor solution	1338:1385	a continuous gelatin hydrogel precursor solution	1338:1385	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	0	30	theme	Tissue	85:90	arg1	Constructs					92:101	Perfusable Tissue Constructs	74:101	Perfusable Tissue Constructs	74:101	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	8	31	theme	proposed	1945:1952	arg1	method					1954:1959	The proposed method	1941:1959	The proposed method	1941:1959	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	8	31	theme	proposed	1945:1952	arg1	method					2005:2010	a solid object sculpting method	1980:2010	a solid object sculpting method	1980:2010	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	1	32	theme	growing	167:173	arg1	field					175:179	a rapidly growing field	157:179	a rapidly growing field	157:179	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	32	theme	growing	167:173	arg1	engineering					142:152	Tissue engineering	135:152	Tissue engineering	135:152	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	2	33	theme	cell-laden	557:566	arg1	constructs					577:586	custom biomedical acellular and cell-laden hydrogel constructs	525:586	custom biomedical acellular and cell-laden hydrogel constructs	525:586	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	5	34	theme	precursor	1368:1376	arg1	solution					1378:1385	a continuous gelatin hydrogel precursor solution	1338:1385	a continuous gelatin hydrogel precursor solution	1338:1385	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	8	35	theme	hydrogel	2119:2126	arg1	matrix					2128:2133	excess composite hydrogel matrix	2102:2133	excess composite hydrogel matrix	2102:2133	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	1	36	theme	custom	373:378	arg1	shapes					380:385	custom shapes	373:385	custom shapes	373:385	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	2	37	theme	acellular	543:551	arg1	constructs					577:586	custom biomedical acellular and cell-laden hydrogel constructs	525:586	custom biomedical acellular and cell-laden hydrogel constructs	525:586	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	7	38	theme	hydrogel	1804:1811	arg1	matrices					1813:1820	the microgel composite hydrogel matrices	1781:1820	the microgel composite hydrogel matrices	1781:1820	Cell-laden constructs with printed features are also evaluated; the microgel composite hydrogel matrices support cell activity, and printed channels enhance proliferation compared to solid constructs even in static culture.					
31948226	9	39	theme	alginate	2183:2190	arg1	solution					2192:2199	aqueous alginate solution	2175:2199	aqueous alginate solution	2175:2199	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	9	39	theme	alginate	2183:2190	arg1	ink					2226:2228	a sacrificial ink	2212:2228	a sacrificial ink	2212:2228	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	5	40	theme	hydrogel	1443:1450	arg1	matrix					1452:1457	the composite hydrogel matrix	1429:1457	the composite hydrogel matrix	1429:1457	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	0	41	theme	Cross-Linkable	0:13	arg1	Bath					41:44	Cross-Linkable Microgel Composite Matrix Bath	0:44	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.	0:133	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	7	42	theme	microgel	1785:1792	arg1	matrices					1813:1820	the microgel composite hydrogel matrices	1781:1820	the microgel composite hydrogel matrices	1781:1820	Cell-laden constructs with printed features are also evaluated; the microgel composite hydrogel matrices support cell activity, and printed channels enhance proliferation compared to solid constructs even in static culture.					
31948226	2	43	theme	embedded	457:464	arg1	methodology					475:485	A versatile embedded printing methodology	445:485	A versatile embedded printing methodology	445:485	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	7	44	theme	static	1925:1930	arg1	culture					1932:1938	static culture	1925:1938	static culture	1925:1938	Cell-laden constructs with printed features are also evaluated; the microgel composite hydrogel matrices support cell activity, and printed channels enhance proliferation compared to solid constructs even in static culture.					
31948226	5	45	theme	matrix	1409:1414	arg1	bath					1416:1419	the composite matrix bath	1395:1419	the composite matrix bath	1395:1419	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	0	46	theme	Composite	24:32	arg1	Bath					41:44	Cross-Linkable Microgel Composite Matrix Bath	0:44	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.	0:133	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	5	47	theme	gellan	1265:1270	arg1	microgels					1310:1318	gellan or enzymatically cross-linked gelatin microgels	1265:1318	gellan or enzymatically cross-linked gelatin microgels	1265:1318	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	3	48	theme	hydrogel	708:715	arg1	bath					734:737	a biocompatible hydrogel precursor matrix bath	692:737	a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features	692:799	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	1	49	theme	physical	314:321	arg1	cavities					363:370	cavities	363:370	cavities	363:370	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	49	theme	physical	314:321	arg1	features					323:330	internal and external physical features	292:330	internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions	292:442	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	49	theme	physical	314:321	arg1	material					410:417	spatially varying material and/or cell compositions	392:442	material	410:417	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	49	theme	physical	314:321	arg1	channels					353:360	perfusable channels	342:360	perfusable channels	342:360	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	49	theme	physical	314:321	arg1	shapes					380:385	custom shapes	373:385	custom shapes	373:385	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	9	50	theme	sacrificial	2214:2224	arg1	solution					2192:2199	aqueous alginate solution	2175:2199	aqueous alginate solution	2175:2199	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	9	50	theme	sacrificial	2214:2224	arg1	ink					2226:2228	a sacrificial ink	2212:2228	a sacrificial ink	2212:2228	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	5	51	used	utilized	1324:1331	arg2	microgels					1310:1318	gellan or enzymatically cross-linked gelatin microgels	1265:1318	gellan or enzymatically cross-linked gelatin microgels	1265:1318	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	0	52	theme	Embedded	50:57	arg1	Bioprinting					59:69	Embedded Bioprinting	50:69	Embedded Bioprinting of Perfusable Tissue Constructs	50:101	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	5	53	theme	gelatin	1501:1507	arg1	phase					1509:1513	the continuous gelatin phase	1486:1513	the continuous gelatin phase	1486:1513	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	3	54	theme	matrix	727:732	arg1	bath					734:737	a biocompatible hydrogel precursor matrix bath	692:737	a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features	692:799	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	1	55	theme	tissue	254:259	arg1	analogues					261:269	cell-laden tissue analogues	243:269	cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions	243:442	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	4	56	theme	hydrogel	1130:1137	arg1	precursor					1139:1147	a fluid hydrogel precursor	1122:1147	a fluid hydrogel precursor	1122:1147	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	8	57	theme	composite	2109:2117	arg1	matrix					2128:2133	excess composite hydrogel matrix	2102:2133	excess composite hydrogel matrix	2102:2133	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	8	58	theme	object	1988:1993	arg1	method					1954:1959	The proposed method	1941:1959	The proposed method	1941:1959	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	8	58	theme	object	1988:1993	arg1	method					2005:2010	a solid object sculpting method	1980:2010	a solid object sculpting method	1980:2010	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	9	59	theme	printing	2294:2301	arg1	resolution					2303:2312	better printing resolution	2287:2312	better printing resolution	2287:2312	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	6	60	theme	discrete	1577:1584	arg1	channels					1586:1593	discrete channels	1577:1593	discrete channels	1577:1593	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	6	61	theme	features	1558:1565	arg1	features					1558:1565	features	1558:1565	features including discrete channels, junctions, networks, and external contours	1558:1637	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	6	61	theme	features	1558:1565	arg1	channels					1586:1593	discrete channels	1577:1593	discrete channels	1577:1593	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	6	61	theme	features	1558:1565	arg1	contours					1630:1637	external contours	1621:1637	external contours	1621:1637	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	6	61	theme	features	1558:1565	arg1	variety					1547:1553	A variety	1545:1553	A variety of features including discrete channels, junctions, networks, and external contours	1545:1637	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	6	61	theme	features	1558:1565	arg1	junctions					1596:1604	junctions	1596:1604	junctions	1596:1604	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	6	61	theme	features	1558:1565	arg1	networks					1607:1614	networks	1607:1614	networks	1607:1614	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	1	62	theme	wide	278:281	arg1	cavities					363:370	cavities	363:370	cavities	363:370	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	62	theme	wide	278:281	arg1	shapes					380:385	custom shapes	373:385	custom shapes	373:385	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	62	theme	wide	278:281	arg1	material					410:417	spatially varying material and/or cell compositions	392:442	material	410:417	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	62	theme	wide	278:281	arg1	channels					353:360	perfusable channels	342:360	perfusable channels	342:360	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	62	theme	wide	278:281	arg1	range					283:287	a wide range	276:287	a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions	276:442	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	2	63	theme	matrix	636:641	arg1	bath					643:646	a biocompatible microgel composite matrix bath	601:646	a biocompatible microgel composite matrix bath	601:646	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	7	64	theme	printed	1849:1855	arg1	channels					1857:1864	printed channels	1849:1864	printed channels	1849:1864	Cell-laden constructs with printed features are also evaluated; the microgel composite hydrogel matrices support cell activity, and printed channels enhance proliferation compared to solid constructs even in static culture.					
31948226	8	65	theme	excess	2102:2107	arg1	matrix					2128:2133	excess composite hydrogel matrix	2102:2133	excess composite hydrogel matrix	2102:2133	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	3	66	theme	matrix	822:827	arg1	bath					829:832	the matrix bath	818:832	the matrix bath	818:832	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	6	67	theme	external	1621:1628	arg1	contours					1630:1637	external contours	1621:1637	external contours	1621:1637	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	1	68	theme	advanced	197:204	arg1	technologies					218:229	advanced fabrication technologies	197:229	advanced fabrication technologies	197:229	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	4	69	theme	matrix	983:988	arg1	material					995:1002	The composite matrix bath material	969:1002	The composite matrix bath material	969:1002	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	1	70	theme	varying	402:408	arg1	material					410:417	spatially varying material and/or cell compositions	392:442	material	410:417	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	2	71	theme	hydrogel	568:575	arg1	constructs					577:586	custom biomedical acellular and cell-laden hydrogel constructs	525:586	custom biomedical acellular and cell-laden hydrogel constructs	525:586	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	4	72	theme	jammed	1016:1021	arg1	microgels					1061:1069	microgels	1061:1069	microgels	1061:1069	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	4	72	theme	jammed	1016:1021	arg1	microparticles					1045:1058	jammed cross-linked hydrogel microparticles	1016:1058	jammed cross-linked hydrogel microparticles (microgels)	1016:1070	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	8	73	theme	sacrificial	2063:2073	arg1	ink					2075:2077	sacrificial ink	2063:2077	sacrificial ink	2063:2077	The proposed method can be expanded as a solid object sculpting method to sculpt external contours by printing a shell of sacrificial ink and further discarding excess composite hydrogel matrix after printing and cross-linking.					
31948226	7	74	theme	Cell-laden	1717:1726	arg1	constructs					1728:1737	Cell-laden constructs	1717:1737	Cell-laden constructs with printed features	1717:1759	Cell-laden constructs with printed features are also evaluated; the microgel composite hydrogel matrices support cell activity, and printed channels enhance proliferation compared to solid constructs even in static culture.					
31948226	2	75	theme	microgel	617:624	arg1	bath					643:646	a biocompatible microgel composite matrix bath	601:646	a biocompatible microgel composite matrix bath	601:646	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	1	76	theme	Tissue	135:140	arg1	field					175:179	a rapidly growing field	157:179	a rapidly growing field	157:179	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	76	theme	Tissue	135:140	arg1	engineering					142:152	Tissue engineering	135:152	Tissue engineering	135:152	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	4	77	theme	hydrogel	1236:1243	arg1	phase					1213:1217	the continuous phase	1198:1217	the continuous phase of the composite hydrogel	1198:1243	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	0	78	theme	Perfusable	74:83	arg1	Constructs					92:101	Perfusable Tissue Constructs	74:101	Perfusable Tissue Constructs	74:101	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	3	79	theme	sacrificial	651:661	arg1	material					663:670	A sacrificial material	649:670	A sacrificial material	649:670	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	6	80	theme	matrix	1680:1685	arg1	bath					1687:1690	the proposed composite matrix bath	1657:1690	the proposed composite matrix bath using embedded printing	1657:1714	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	3	81	theme	perfusable	906:915	arg1	channels					917:924	perfusable channels	906:924	perfusable channels within the bulk composite hydrogel matrix	906:966	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	5	82	theme	continuous	1340:1349	arg1	solution					1378:1385	a continuous gelatin hydrogel precursor solution	1338:1385	a continuous gelatin hydrogel precursor solution	1338:1385	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	0	83	theme	Constructs	92:101	arg1	Sculpting					107:115	Sculpting	107:115	Sculpting of Solid Objects	107:132	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	0	83	theme	Constructs	92:101	arg1	Bioprinting					59:69	Embedded Bioprinting	50:69	Embedded Bioprinting of Perfusable Tissue Constructs	50:101	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	6	84	theme	proposed	1661:1668	arg1	bath					1687:1690	the proposed composite matrix bath	1657:1690	the proposed composite matrix bath using embedded printing	1657:1714	A variety of features including discrete channels, junctions, networks, and external contours are fabricated in the proposed composite matrix bath using embedded printing.					
31948226	3	85	theme	hydrogel	952:959	arg1	matrix					961:966	the bulk composite hydrogel matrix	933:966	the bulk composite hydrogel matrix	933:966	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	5	86	theme	hydrogel	1359:1366	arg1	solution					1378:1385	a continuous gelatin hydrogel precursor solution	1338:1385	a continuous gelatin hydrogel precursor solution	1338:1385	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	7	87	with	constructs	1728:1737	arg1	features					1752:1759	printed features	1744:1759	printed features	1744:1759	Cell-laden constructs with printed features are also evaluated; the microgel composite hydrogel matrices support cell activity, and printed channels enhance proliferation compared to solid constructs even in static culture.					
31948226	3	88	theme	bulk	937:940	arg1	matrix					961:966	the bulk composite hydrogel matrix	933:966	the bulk composite hydrogel matrix	933:966	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	0	89	theme	Solid	120:124	arg1	Objects					126:132	Solid Objects	120:132	Solid Objects	120:132	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	7	90	theme	cell	1830:1833	arg1	activity					1835:1842	cell activity	1830:1842	cell activity	1830:1842	Cell-laden constructs with printed features are also evaluated; the microgel composite hydrogel matrices support cell activity, and printed channels enhance proliferation compared to solid constructs even in static culture.					
31948226	2	91	theme	biomedical	532:541	arg1	constructs					577:586	custom biomedical acellular and cell-laden hydrogel constructs	525:586	custom biomedical acellular and cell-laden hydrogel constructs	525:586	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	9	92	theme	aqueous	2175:2181	arg1	solution					2192:2199	aqueous alginate solution	2175:2199	aqueous alginate solution	2175:2199	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	9	92	theme	aqueous	2175:2181	arg1	ink					2226:2228	a sacrificial ink	2212:2228	a sacrificial ink	2212:2228	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	5	93	theme	composite	1433:1441	arg1	matrix					1452:1457	the composite hydrogel matrix	1429:1457	the composite hydrogel matrix	1429:1457	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	4	94	theme	hydrogel	1036:1043	arg1	microgels					1061:1069	microgels	1061:1069	microgels	1061:1069	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	4	94	theme	hydrogel	1036:1043	arg1	microparticles					1045:1058	jammed cross-linked hydrogel microparticles	1016:1058	jammed cross-linked hydrogel microparticles (microgels)	1016:1070	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	0	95	theme	Microgel	15:22	arg1	Bath					41:44	Cross-Linkable Microgel Composite Matrix Bath	0:44	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.	0:133	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	2	96	theme	printing	466:473	arg1	methodology					475:485	A versatile embedded printing methodology	445:485	A versatile embedded printing methodology	445:485	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	9	97	theme	advanced	2236:2243	arg1	materials					2257:2265	more advanced sacrificial materials	2231:2265	more advanced sacrificial materials	2231:2265	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
31948226	5	98	theme	composite	1399:1407	arg1	bath					1416:1419	the composite matrix bath	1395:1419	the composite matrix bath	1395:1419	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	0	99	theme	Matrix	34:39	arg1	Bath					41:44	Cross-Linkable Microgel Composite Matrix Bath	0:44	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.	0:133	Cross-Linkable Microgel Composite Matrix Bath for Embedded Bioprinting of Perfusable Tissue Constructs and Sculpting of Solid Objects.					
31948226	1	100	theme	internal	292:299	arg1	cavities					363:370	cavities	363:370	cavities	363:370	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	100	theme	internal	292:299	arg1	features					323:330	internal and external physical features	292:330	internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions	292:442	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	100	theme	internal	292:299	arg1	material					410:417	spatially varying material and/or cell compositions	392:442	material	410:417	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	100	theme	internal	292:299	arg1	channels					353:360	perfusable channels	342:360	perfusable channels	342:360	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	100	theme	internal	292:299	arg1	shapes					380:385	custom shapes	373:385	custom shapes	373:385	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	7	101	theme	composite	1794:1802	arg1	matrices					1813:1820	the microgel composite hydrogel matrices	1781:1820	the microgel composite hydrogel matrices	1781:1820	Cell-laden constructs with printed features are also evaluated; the microgel composite hydrogel matrices support cell activity, and printed channels enhance proliferation compared to solid constructs even in static culture.					
31948226	2	102	theme	versatile	447:455	arg1	methodology					475:485	A versatile embedded printing methodology	445:485	A versatile embedded printing methodology	445:485	A versatile embedded printing methodology is proposed in this work for creating custom biomedical acellular and cell-laden hydrogel constructs by utilizing a biocompatible microgel composite matrix bath.					
31948226	3	103	theme	biocompatible	694:706	arg1	bath					734:737	a biocompatible hydrogel precursor matrix bath	692:737	a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features	692:799	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	1	104	theme	external	305:312	arg1	cavities					363:370	cavities	363:370	cavities	363:370	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	104	theme	external	305:312	arg1	features					323:330	internal and external physical features	292:330	internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions	292:442	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	104	theme	external	305:312	arg1	material					410:417	spatially varying material and/or cell compositions	392:442	material	410:417	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	104	theme	external	305:312	arg1	channels					353:360	perfusable channels	342:360	perfusable channels	342:360	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	104	theme	external	305:312	arg1	shapes					380:385	custom shapes	373:385	custom shapes	373:385	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	4	105	theme	cross-linked	1023:1034	arg1	microgels					1061:1069	microgels	1061:1069	microgels	1061:1069	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	4	105	theme	cross-linked	1023:1034	arg1	microparticles					1045:1058	jammed cross-linked hydrogel microparticles	1016:1058	jammed cross-linked hydrogel microparticles (microgels)	1016:1070	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	1	106	theme	features	323:330	arg1	cavities					363:370	cavities	363:370	cavities	363:370	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	106	theme	features	323:330	arg1	shapes					380:385	custom shapes	373:385	custom shapes	373:385	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	106	theme	features	323:330	arg1	material					410:417	spatially varying material and/or cell compositions	392:442	material	410:417	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	106	theme	features	323:330	arg1	channels					353:360	perfusable channels	342:360	perfusable channels	342:360	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	106	theme	features	323:330	arg1	range					283:287	a wide range	276:287	a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions	276:442	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	4	107	link	cross-linked	1023:1034	arg1	microgels					1061:1069	microgels	1061:1069	microgels	1061:1069	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	4	107	link	cross-linked	1023:1034	arg1	microparticles					1045:1058	jammed cross-linked hydrogel microparticles	1016:1058	jammed cross-linked hydrogel microparticles (microgels)	1016:1070	The composite matrix bath material consists of jammed cross-linked hydrogel microparticles (microgels) to control rheology during fabrication along with a fluid hydrogel precursor, which is cross-linked after fabrication to form the continuous phase of the composite hydrogel.					
31948226	5	108	theme	continuous	1490:1499	arg1	phase					1509:1513	the continuous gelatin phase	1486:1513	the continuous gelatin phase	1486:1513	For demonstration, gellan or enzymatically cross-linked gelatin microgels are utilized with a continuous gelatin hydrogel precursor solution to make the composite matrix bath herein; the composite hydrogel matrix is formed by cross-linking the continuous gelatin phase enzymatically after printing.					
31948226	1	109	theme	perfusable	342:351	arg1	channels					353:360	perfusable channels	342:360	perfusable channels	342:360	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	3	110	theme	precursor	717:725	arg1	bath					734:737	a biocompatible hydrogel precursor matrix bath	692:737	a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features	692:799	A sacrificial material is patterned within a biocompatible hydrogel precursor matrix bath using extrusion printing to create three-dimensional features; after printing, the matrix bath is cross-linked, and the sacrificial material is flushed away to create perfusable channels within the bulk composite hydrogel matrix.					
31948226	1	111	theme	cell-laden	243:252	arg1	analogues					261:269	cell-laden tissue analogues	243:269	cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions	243:442	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	112	with	analogues	261:269	arg1	cavities					363:370	cavities	363:370	cavities	363:370	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	112	with	analogues	261:269	arg1	shapes					380:385	custom shapes	373:385	custom shapes	373:385	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	112	with	analogues	261:269	arg1	material					410:417	spatially varying material and/or cell compositions	392:442	material	410:417	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	112	with	analogues	261:269	arg1	channels					353:360	perfusable channels	342:360	perfusable channels	342:360	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	1	112	with	analogues	261:269	arg1	range					283:287	a wide range	276:287	a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions	276:442	Tissue engineering is a rapidly growing field, which requires advanced fabrication technologies to generate cell-laden tissue analogues with a wide range of internal and external physical features including perfusable channels, cavities, custom shapes, and spatially varying material and/or cell compositions.					
31948226	9	113	theme	sacrificial	2245:2255	arg1	materials					2257:2265	more advanced sacrificial materials	2231:2265	more advanced sacrificial materials	2231:2265	While aqueous alginate solution is used as a sacrificial ink, more advanced sacrificial materials can be utilized for better printing resolution.					
33766612	0	0	theme	modulation	89:98	arg1	effects					100:106	its modulation effects	85:106	its modulation effects on gut microbiota	85:124	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	3	1	from	carbohydrate	406:417	arg1	SFP					456:458	SFP	456:458	SFP	456:458	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	10	2	theme	intestinal	1542:1551	arg1	health					1553:1558	intestinal health	1542:1558	intestinal health	1542:1558	These results showed that SFP could be utilized by microbiota in human feces, and may have the potential to improve intestinal health.					
33766612	5	3	theme	typical	663:669	arg1	polysaccharide					679:692	a typical sulfate polysaccharide	661:692	a typical sulfate polysaccharide with relative smooth surface and regular shape	661:739	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	5	3	theme	typical	663:669	arg1	SFP					653:655	SFP	653:655	SFP	653:655	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	5	4	theme	relative	699:706	arg1	surface					715:721	relative smooth surface	699:721	relative smooth surface	699:721	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	9	5	dep	Ruminococcaceae_UCG-014	1224:1246	arg1	increased					1249:1257	increased	1249:1257	increased by 177.78%	1249:1268	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	5	6	theme	UV	602:603	arg1	spectrum					605:612	UV spectrum	602:612	UV spectrum	602:612	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	6	7	theme	in	748:749	arg1	fermentation					757:768	in vitro fermentation	748:768	in vitro fermentation for 24 h, the pH value of fermentation medium	748:814	After in vitro fermentation for 24 h, the pH value of fermentation medium declined significantly (p < 0.05), utilization of carbohydrate was 53.17%.					
33766612	1	8	theme	Sargassum	163:171	arg1	SFP					184:186	SFP	184:186	SFP	184:186	In this study, polysaccharides from Sargassum fusiforme (SFP) were obtained by cellulase assisted hot water extraction.					
33766612	1	8	theme	Sargassum	163:171	arg1	fusiforme					173:181	Sargassum fusiforme	163:181	Sargassum fusiforme (SFP)	163:187	In this study, polysaccharides from Sargassum fusiforme (SFP) were obtained by cellulase assisted hot water extraction.					
33766612	3	9	from	protein	420:426	arg1	SFP					456:458	SFP	456:458	SFP	456:458	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	5	10	theme	spectrum	605:612	arg1	results					633:639	UV spectrum, FTIR, SEM and AFM results	602:639	UV spectrum, FTIR, SEM and AFM results	602:639	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	6	11	theme	pH	784:785	arg1	24 h					774:777	24 h	774:777	24 h	774:777	After in vitro fermentation for 24 h, the pH value of fermentation medium declined significantly (p < 0.05), utilization of carbohydrate was 53.17%.					
33766612	6	11	theme	pH	784:785	arg1	value					787:791	the pH value	780:791	the pH value of fermentation medium	780:814	After in vitro fermentation for 24 h, the pH value of fermentation medium declined significantly (p < 0.05), utilization of carbohydrate was 53.17%.					
33766612	10	12	theme	human	1491:1495	arg1	feces					1497:1501	human feces	1491:1501	human feces	1491:1501	These results showed that SFP could be utilized by microbiota in human feces, and may have the potential to improve intestinal health.					
33766612	5	13	theme	regular	727:733	arg1	shape					735:739	regular shape	727:739	regular shape	727:739	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	6	14	theme	medium	809:814	arg1	24 h					774:777	24 h	774:777	24 h	774:777	After in vitro fermentation for 24 h, the pH value of fermentation medium declined significantly (p < 0.05), utilization of carbohydrate was 53.17%.					
33766612	6	14	theme	medium	809:814	arg1	value					787:791	the pH value	780:791	the pH value of fermentation medium	780:814	After in vitro fermentation for 24 h, the pH value of fermentation medium declined significantly (p < 0.05), utilization of carbohydrate was 53.17%.					
33766612	9	15	theme	Prevotella_9	1341:1352	arg1	abundance					1328:1336	the abundance	1324:1336	the abundance of Prevotella_9 (decreased by 34.54%) and Blautia	1324:1386	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	2	16	theme	structural	273:282	arg1	characteristics					284:298	structural characteristics	273:298	structural characteristics	273:298	The chemical composition, structural characteristics, and in vitro fermentation properties of SFP were investigated.					
33766612	9	17	dep	Faecalibacterium	1067:1082	arg1	increased					1085:1093	increased	1085:1093	increased by 49.07% compared with the Blank24 group	1085:1135	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	3	18	theme	acid	436:439	arg1	contents					388:395	the contents	384:395	the contents of total carbohydrate, protein, uronic acid and sulfate in SFP	384:458	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	3	18	theme	acid	436:439	arg1	%					470:470	83.25%	465:470	83.25%	465:470	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	0	19	theme	gut	111:113	arg1	microbiota					115:124	gut microbiota	111:124	gut microbiota	111:124	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	3	20	from	contents	388:395	arg1	SFP					456:458	SFP	456:458	SFP	456:458	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	5	21	theme	smooth	708:713	arg1	surface					715:721	relative smooth surface	699:721	relative smooth surface	699:721	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	9	22	theme	Blank24	1123:1129	arg1	group					1131:1135	the Blank24 group	1119:1135	the Blank24 group	1119:1135	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	9	23	dep	Lactobacillus	1276:1288	arg1	increased					1291:1299	increased	1291:1299	increased by 400.00%	1291:1310	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	2	24	theme	fermentation	314:325	arg1	properties					327:336	in vitro fermentation properties	305:336	in vitro fermentation properties	305:336	The chemical composition, structural characteristics, and in vitro fermentation properties of SFP were investigated.					
33766612	0	25	theme	In	0:1	arg1	characteristics					22:36	In vitro fermentation characteristics	0:36	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme	0:79	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	9	26	theme	genus	1413:1417	arg1	level					1419:1423	genus level	1413:1423	genus level	1413:1423	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	3	27	theme	uronic	429:434	arg1	acid					436:439	uronic acid	429:439	uronic acid	429:439	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	9	28	dep	Phascolarctobacterium	1139:1159	arg1	increased					1162:1170	increased	1162:1170	increased by 88.06%	1162:1180	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	5	29	theme	AFM	629:631	arg1	results					633:639	UV spectrum, FTIR, SEM and AFM results	602:639	UV spectrum, FTIR, SEM and AFM results	602:639	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	9	30	dep	increased	1040:1048	arg1	decreased					1314:1322	decreased	1314:1322	decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level	1314:1423	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	2	31	theme	in	305:306	arg1	properties					327:336	in vitro fermentation properties	305:336	in vitro fermentation properties	305:336	The chemical composition, structural characteristics, and in vitro fermentation properties of SFP were investigated.					
33766612	0	32	theme	fermentation	9:20	arg1	characteristics					22:36	In vitro fermentation characteristics	0:36	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme	0:79	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	9	33	dep	Bifidobacterium	1184:1198	arg1	increased					1201:1209	increased	1201:1209	increased by 139.13%	1201:1220	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	7	34	theme	SCFAs	913:917	arg1	contents					895:902	The contents	891:902	The contents of total SCFAs	891:917	The contents of total SCFAs increased by 10.77 times.					
33766612	6	35	dep	in	748:749	arg1	vitro					751:755	vitro	751:755	vitro	751:755	After in vitro fermentation for 24 h, the pH value of fermentation medium declined significantly (p < 0.05), utilization of carbohydrate was 53.17%.					
33766612	4	36	theme	molecular	570:578	arg1	weight					580:585	molecular weight	570:585	molecular weight of 255.83 kDa	570:599	It mainly consisted of fucose glucose and galactose, with molecular weight of 255.83 kDa.					
33766612	0	37	theme	polysaccharide	41:54	arg1	characteristics					22:36	In vitro fermentation characteristics	0:36	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme	0:79	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	0	37	theme	polysaccharide	41:54	arg1	effects					100:106	its modulation effects	85:106	its modulation effects on gut microbiota	85:124	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	4	38	theme	255.83 kDa	590:599	arg1	weight					580:585	molecular weight	570:585	molecular weight of 255.83 kDa	570:599	It mainly consisted of fucose glucose and galactose, with molecular weight of 255.83 kDa.					
33766612	3	39	theme	sulfate	445:451	arg1	contents					388:395	the contents	384:395	the contents of total carbohydrate, protein, uronic acid and sulfate in SFP	384:458	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	3	39	theme	sulfate	445:451	arg1	%					470:470	83.25%	465:470	83.25%	465:470	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	3	40	theme	protein	420:426	arg1	contents					388:395	the contents	384:395	the contents of total carbohydrate, protein, uronic acid and sulfate in SFP	384:458	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	3	40	theme	protein	420:426	arg1	%					470:470	83.25%	465:470	83.25%	465:470	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	6	41	theme	carbohydrate	866:877	arg1	utilization					851:861	utilization	851:861	utilization of carbohydrate	851:877	After in vitro fermentation for 24 h, the pH value of fermentation medium declined significantly (p < 0.05), utilization of carbohydrate was 53.17%.					
33766612	8	42	theme	microbiota	999:1008	arg1	composition					1010:1020	the microbiota composition	995:1020	the microbiota composition	995:1020	Moreover, SFP fermentation could change obviously the microbiota composition.					
33766612	6	43	dep	declined	816:823	arg1	p < 0.05					840:847	p < 0.05	840:847	p < 0.05	840:847	After in vitro fermentation for 24 h, the pH value of fermentation medium declined significantly (p < 0.05), utilization of carbohydrate was 53.17%.					
33766612	3	44	from	sulfate	445:451	arg1	SFP					456:458	SFP	456:458	SFP	456:458	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	1	45	theme	cellulase	206:214	arg1	extraction					235:244	cellulase assisted hot water extraction	206:244	cellulase assisted hot water extraction	206:244	In this study, polysaccharides from Sargassum fusiforme (SFP) were obtained by cellulase assisted hot water extraction.					
33766612	0	46	theme	Sargassum	61:69	arg1	fusiforme					71:79	Sargassum fusiforme	61:79	Sargassum fusiforme	61:79	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	5	47	theme	FTIR	615:618	arg1	results					633:639	UV spectrum, FTIR, SEM and AFM results	602:639	UV spectrum, FTIR, SEM and AFM results	602:639	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	0	48	from	fusiforme	71:79	arg1	polysaccharide					41:54	polysaccharide	41:54	polysaccharide from Sargassum fusiforme	41:79	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	0	48	from	fusiforme	71:79	arg1	characteristics					22:36	In vitro fermentation characteristics	0:36	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme	0:79	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	0	48	from	fusiforme	71:79	arg1	effects					100:106	its modulation effects	85:106	its modulation effects on gut microbiota	85:124	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	6	49	theme	fermentation	796:807	arg1	medium					809:814	fermentation medium	796:814	fermentation medium	796:814	After in vitro fermentation for 24 h, the pH value of fermentation medium declined significantly (p < 0.05), utilization of carbohydrate was 53.17%.					
33766612	2	50	dep	in	305:306	arg1	vitro					308:312	vitro	308:312	vitro	308:312	The chemical composition, structural characteristics, and in vitro fermentation properties of SFP were investigated.					
33766612	3	51	theme	carbohydrate	406:417	arg1	contents					388:395	the contents	384:395	the contents of total carbohydrate, protein, uronic acid and sulfate in SFP	384:458	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	3	51	theme	carbohydrate	406:417	arg1	%					470:470	83.25%	465:470	83.25%	465:470	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	10	52	contain	have	1512:1515	arg1	SFP					1452:1454	SFP	1452:1454	SFP	1452:1454	These results showed that SFP could be utilized by microbiota in human feces, and may have the potential to improve intestinal health.					
33766612	10	52	contain	have	1512:1515	arg2	potential					1521:1529	the potential to improve intestinal health	1517:1558	the potential to improve intestinal health	1517:1558	These results showed that SFP could be utilized by microbiota in human feces, and may have the potential to improve intestinal health.					
33766612	8	53	theme	SFP	955:957	arg1	fermentation					959:970	SFP fermentation	955:970	SFP fermentation	955:970	Moreover, SFP fermentation could change obviously the microbiota composition.					
33766612	3	54	from	SFP	456:458	arg1	contents					388:395	the contents	384:395	the contents of total carbohydrate, protein, uronic acid and sulfate in SFP	384:458	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	3	54	from	SFP	456:458	arg1	%					470:470	83.25%	465:470	83.25%	465:470	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	2	55	theme	SFP	341:343	arg1	characteristics					284:298	structural characteristics	273:298	structural characteristics	273:298	The chemical composition, structural characteristics, and in vitro fermentation properties of SFP were investigated.					
33766612	2	55	theme	SFP	341:343	arg1	properties					327:336	in vitro fermentation properties	305:336	in vitro fermentation properties	305:336	The chemical composition, structural characteristics, and in vitro fermentation properties of SFP were investigated.					
33766612	2	55	theme	SFP	341:343	arg1	composition					260:270	The chemical composition	247:270	The chemical composition	247:270	The chemical composition, structural characteristics, and in vitro fermentation properties of SFP were investigated.					
33766612	5	56	with	polysaccharide	679:692	arg1	surface					715:721	relative smooth surface	699:721	relative smooth surface	699:721	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	5	56	with	polysaccharide	679:692	arg1	shape					735:739	regular shape	727:739	regular shape	727:739	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	5	57	theme	SEM	621:623	arg1	results					633:639	UV spectrum, FTIR, SEM and AFM results	602:639	UV spectrum, FTIR, SEM and AFM results	602:639	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	9	58	dep	decreased	1314:1322	arg1	decreased					1389:1397	decreased	1389:1397	decreased by 40.79%	1389:1407	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	9	59	theme	Faecalibacterium	1067:1082	arg1	Bifidobacterium					1184:1198	Bifidobacterium	1184:1198	Bifidobacterium (increased by 139.13%)	1184:1221	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	9	59	theme	Faecalibacterium	1067:1082	arg1	Phascolarctobacterium					1139:1159	Phascolarctobacterium	1139:1159	Phascolarctobacterium (increased by 88.06%)	1139:1181	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	9	59	theme	Faecalibacterium	1067:1082	arg1	abundance					1054:1062	the abundance	1050:1062	the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group)	1050:1136	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	9	59	theme	Faecalibacterium	1067:1082	arg1	Ruminococcaceae_UCG-014					1224:1246	Ruminococcaceae_UCG-014	1224:1246	Ruminococcaceae_UCG-014 (increased by 177.78%)	1224:1269	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	9	59	theme	Faecalibacterium	1067:1082	arg1	Lactobacillus					1276:1288	Lactobacillus	1276:1288	Lactobacillus (increased by 400.00%)	1276:1311	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
33766612	10	60	used	utilized	1465:1472	arg2	SFP					1452:1454	SFP	1452:1454	SFP	1452:1454	These results showed that SFP could be utilized by microbiota in human feces, and may have the potential to improve intestinal health.					
33766612	3	61	from	acid	436:439	arg1	SFP					456:458	SFP	456:458	SFP	456:458	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	1	62	theme	assisted	216:223	arg1	extraction					235:244	cellulase assisted hot water extraction	206:244	cellulase assisted hot water extraction	206:244	In this study, polysaccharides from Sargassum fusiforme (SFP) were obtained by cellulase assisted hot water extraction.					
33766612	4	63	dep	fucose	535:540	arg1	glucose					542:548	glucose	542:548	glucose	542:548	It mainly consisted of fucose glucose and galactose, with molecular weight of 255.83 kDa.					
33766612	2	64	theme	chemical	251:258	arg1	composition					260:270	The chemical composition	247:270	The chemical composition	247:270	The chemical composition, structural characteristics, and in vitro fermentation properties of SFP were investigated.					
33766612	1	65	theme	hot	225:227	arg1	extraction					235:244	cellulase assisted hot water extraction	206:244	cellulase assisted hot water extraction	206:244	In this study, polysaccharides from Sargassum fusiforme (SFP) were obtained by cellulase assisted hot water extraction.					
33766612	5	66	theme	sulfate	671:677	arg1	polysaccharide					679:692	a typical sulfate polysaccharide	661:692	a typical sulfate polysaccharide with relative smooth surface and regular shape	661:739	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	5	66	theme	sulfate	671:677	arg1	SFP					653:655	SFP	653:655	SFP	653:655	UV spectrum, FTIR, SEM and AFM results showed that SFP was a typical sulfate polysaccharide with relative smooth surface and regular shape.					
33766612	0	67	from	effects	100:106	arg1	fusiforme					71:79	Sargassum fusiforme	61:79	Sargassum fusiforme	61:79	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	0	67	from	effects	100:106	arg1	microbiota					115:124	gut microbiota	111:124	gut microbiota	111:124	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	7	68	theme	total	907:911	arg1	SCFAs					913:917	total SCFAs	907:917	total SCFAs	907:917	The contents of total SCFAs increased by 10.77 times.					
33766612	1	69	theme	water	229:233	arg1	extraction					235:244	cellulase assisted hot water extraction	206:244	cellulase assisted hot water extraction	206:244	In this study, polysaccharides from Sargassum fusiforme (SFP) were obtained by cellulase assisted hot water extraction.					
33766612	3	70	theme	total	400:404	arg1	carbohydrate					406:417	total carbohydrate	400:417	total carbohydrate	400:417	Results showed that the contents of total carbohydrate, protein, uronic acid and sulfate in SFP were 83.25%, 1.42%, 12.80% and 7.81%, respectively.					
33766612	0	71	from	characteristics	22:36	arg1	fusiforme					71:79	Sargassum fusiforme	61:79	Sargassum fusiforme	61:79	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	0	71	from	characteristics	22:36	arg1	microbiota					115:124	gut microbiota	111:124	gut microbiota	111:124	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	0	72	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro fermentation characteristics of polysaccharide from Sargassum fusiforme and its modulation effects on gut microbiota.					
33766612	1	73	from	fusiforme	173:181	arg1	polysaccharides					142:156	polysaccharides	142:156	polysaccharides from Sargassum fusiforme (SFP)	142:187	In this study, polysaccharides from Sargassum fusiforme (SFP) were obtained by cellulase assisted hot water extraction.					
33766612	9	74	theme	Blautia	1380:1386	arg1	abundance					1328:1336	the abundance	1324:1336	the abundance of Prevotella_9 (decreased by 34.54%) and Blautia	1324:1386	It significantly increased the abundance of Faecalibacterium (increased by 49.07% compared with the Blank24 group), Phascolarctobacterium (increased by 88.06%), Bifidobacterium (increased by 139.13%), Ruminococcaceae_UCG-014 (increased by 177.78%), and Lactobacillus (increased by 400.00%), decreased the abundance of Prevotella_9 (decreased by 34.54%) and Blautia (decreased by 40.79%) at genus level.					
34732740	6	0	theme	putative	1007:1014	arg1	function					1016:1023	a putative function	1005:1023	a putative function in stabilizing macromolecular complexes	1005:1063	Identification of valines in the alkali-insoluble carbohydrate core suggests a putative function in stabilizing macromolecular complexes.					
34732740	2	1	theme	pathogen	350:357	arg1	fumigatus					371:379	a fungal pathogen Aspergillus fumigatus	341:379	a fungal pathogen Aspergillus fumigatus	341:379	Here we compare the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides.					
34732740	5	2	theme	α-1,3-glucan	824:835	arg1	distribution					808:819	The distribution	804:819	The distribution of α-1,3-glucan in chemically isolated and dynamically distinct domains	804:891	The distribution of α-1,3-glucan in chemically isolated and dynamically distinct domains supports its functional diversity.					
34732740	2	3	theme	fungal	343:348	arg1	fumigatus					371:379	a fungal pathogen Aspergillus fumigatus	341:379	a fungal pathogen Aspergillus fumigatus	341:379	Here we compare the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides.					
34732740	3	4	attach	present	621:627	arg1	phase					643:647	the mobile phase	632:647	the mobile phase	632:647	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	3	4	attach	present	621:627	arg2	galactosaminogalactan					581:601	galactosaminogalactan	581:601	galactosaminogalactan	581:601	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	1	5	theme	carbohydrate-rich	235:251	arg1	composite					253:261	this carbohydrate-rich composite	230:261	this carbohydrate-rich composite	230:261	Vast efforts have been devoted to the development of antifungal drugs targeting the cell wall, but the supramolecular architecture of this carbohydrate-rich composite remains insufficiently understood.					
34732740	4	6	theme	spatial	695:701	arg1	organization					703:714	spatial organization	695:714	spatial organization	695:714	Gene deletion reshuffles the composition and spatial organization of polysaccharides, with significant changes in their dynamics and water accessibility.					
34732740	1	7	theme	composite	253:261	arg1	architecture					214:225	the supramolecular architecture	195:225	the supramolecular architecture of this carbohydrate-rich composite	195:261	Vast efforts have been devoted to the development of antifungal drugs targeting the cell wall, but the supramolecular architecture of this carbohydrate-rich composite remains insufficiently understood.					
34732740	0	8	theme	solid-state	79:89	arg1	NMR					91:93	solid-state NMR	79:93	solid-state NMR	79:93	A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR.					
34732740	4	9	theme	significant	741:751	arg1	changes					753:759	significant changes	741:759	significant changes in their dynamics and water accessibility	741:801	Gene deletion reshuffles the composition and spatial organization of polysaccharides, with significant changes in their dynamics and water accessibility.					
34732740	6	10	theme	carbohydrate	978:989	arg1	core					991:994	the alkali-insoluble carbohydrate core	957:994	the alkali-insoluble carbohydrate core	957:994	Identification of valines in the alkali-insoluble carbohydrate core suggests a putative function in stabilizing macromolecular complexes.					
34732740	5	11	theme	functional	906:915	arg1	diversity					917:925	its functional diversity	902:925	its functional diversity	902:925	The distribution of α-1,3-glucan in chemically isolated and dynamically distinct domains supports its functional diversity.					
34732740	3	12	theme	cells	499:503	arg1	spectroscopy					476:487	High-resolution solid-state NMR spectroscopy	444:487	High-resolution solid-state NMR spectroscopy of intact cells	444:503	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	3	13	theme	NMR	472:474	arg1	spectroscopy					476:487	High-resolution solid-state NMR spectroscopy	444:487	High-resolution solid-state NMR spectroscopy of intact cells	444:503	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	3	14	from	phase	643:647	arg1	present					621:627	present	621:627	present	621:627	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	1	15	theme	antifungal	149:158	arg1	drugs					160:164	antifungal drugs	149:164	antifungal drugs targeting the cell wall	149:188	Vast efforts have been devoted to the development of antifungal drugs targeting the cell wall, but the supramolecular architecture of this carbohydrate-rich composite remains insufficiently understood.					
34732740	6	16	theme	alkali-insoluble	961:976	arg1	core					991:994	the alkali-insoluble carbohydrate core	957:994	the alkali-insoluble carbohydrate core	957:994	Identification of valines in the alkali-insoluble carbohydrate core suggests a putative function in stabilizing macromolecular complexes.					
34732740	6	17	from	Identification	928:941	arg1	core					991:994	the alkali-insoluble carbohydrate core	957:994	the alkali-insoluble carbohydrate core	957:994	Identification of valines in the alkali-insoluble carbohydrate core suggests a putative function in stabilizing macromolecular complexes.					
34732740	1	18	theme	drugs	160:164	arg1	development					134:144	the development	130:144	the development of antifungal drugs targeting the cell wall	130:188	Vast efforts have been devoted to the development of antifungal drugs targeting the cell wall, but the supramolecular architecture of this carbohydrate-rich composite remains insufficiently understood.					
34732740	0	19	theme	molecular	2:10	arg1	vision					12:17	A molecular vision	0:17	A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR.	0:94	A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR.					
34732740	7	20	theme	fungal	1186:1191	arg1	pathogens					1193:1201	fungal pathogens	1186:1201	fungal pathogens	1186:1201	We propose a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses.					
34732740	2	21	theme	cell	318:321	arg1	structure					328:336	the cell wall structure	314:336	the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides	314:441	Here we compare the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides.					
34732740	5	22	theme	distinct	876:883	arg1	domains					885:891	dynamically distinct domains	864:891	dynamically distinct domains	864:891	The distribution of α-1,3-glucan in chemically isolated and dynamically distinct domains supports its functional diversity.					
34732740	7	23	theme	pathogens	1193:1201	arg1	response					1174:1181	the structural response	1159:1181	the structural response of fungal pathogens to stresses	1159:1213	We propose a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses.					
34732740	3	24	theme	High-resolution	444:458	arg1	spectroscopy					476:487	High-resolution solid-state NMR spectroscopy	444:487	High-resolution solid-state NMR spectroscopy of intact cells	444:503	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	7	25	theme	response	1174:1181	arg1	understanding					1142:1154	our understanding	1138:1154	our understanding of the structural response of fungal pathogens to stresses	1138:1213	We propose a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses.					
34732740	3	26	from	present	621:627	arg1	phase					643:647	the mobile phase	632:647	the mobile phase	632:647	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	1	27	theme	cell	180:183	arg1	wall					185:188	the cell wall	176:188	the cell wall	176:188	Vast efforts have been devoted to the development of antifungal drugs targeting the cell wall, but the supramolecular architecture of this carbohydrate-rich composite remains insufficiently understood.					
34732740	7	28	theme	cell	1096:1099	arg1	architecture					1106:1117	cell wall architecture	1096:1117	cell wall architecture	1096:1117	We propose a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses.					
34732740	1	29	theme	Vast	96:99	arg1	efforts					101:107	Vast efforts	96:107	Vast efforts	96:107	Vast efforts have been devoted to the development of antifungal drugs targeting the cell wall, but the supramolecular architecture of this carbohydrate-rich composite remains insufficiently understood.					
34732740	2	30	theme	structural	416:425	arg1	polysaccharides					427:441	major structural polysaccharides	410:441	major structural polysaccharides	410:441	Here we compare the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides.					
34732740	0	31	theme	cell	29:32	arg1	organization					39:50	fungal cell wall organization	22:50	fungal cell wall organization	22:50	A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR.					
34732740	6	32	theme	valines	946:952	arg1	Identification					928:941	Identification	928:941	Identification of valines in the alkali-insoluble carbohydrate core	928:994	Identification of valines in the alkali-insoluble carbohydrate core suggests a putative function in stabilizing macromolecular complexes.					
34732740	4	33	dep	composition	679:689	arg1	the					675:677	the	675:677	the	675:677	Gene deletion reshuffles the composition and spatial organization of polysaccharides, with significant changes in their dynamics and water accessibility.					
34732740	4	34	from	changes	753:759	arg1	accessibility					789:801	water accessibility	783:801	water accessibility	783:801	Gene deletion reshuffles the composition and spatial organization of polysaccharides, with significant changes in their dynamics and water accessibility.					
34732740	4	34	from	changes	753:759	arg1	dynamics					770:777	their dynamics	764:777	their dynamics	764:777	Gene deletion reshuffles the composition and spatial organization of polysaccharides, with significant changes in their dynamics and water accessibility.					
34732740	2	35	theme	major	410:414	arg1	polysaccharides					427:441	major structural polysaccharides	410:441	major structural polysaccharides	410:441	Here we compare the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides.					
34732740	0	36	theme	fungal	22:27	arg1	organization					39:50	fungal cell wall organization	22:50	fungal cell wall organization	22:50	A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR.					
34732740	7	37	theme	structural	1163:1172	arg1	response					1174:1181	the structural response	1159:1181	the structural response of fungal pathogens to stresses	1159:1213	We propose a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses.					
34732740	0	38	theme	organization	39:50	arg1	vision					12:17	A molecular vision	0:17	A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR.	0:94	A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR.					
34732740	6	39	theme	macromolecular	1040:1053	arg1	complexes					1055:1063	macromolecular complexes	1040:1063	macromolecular complexes	1040:1063	Identification of valines in the alkali-insoluble carbohydrate core suggests a putative function in stabilizing macromolecular complexes.					
34732740	3	40	theme	solid-state	460:470	arg1	spectroscopy					476:487	High-resolution solid-state NMR spectroscopy	444:487	High-resolution solid-state NMR spectroscopy of intact cells	444:503	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	3	41	theme	rigid	515:519	arg1	core					521:524	a rigid core	513:524	a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan	513:573	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	0	42	theme	wall	34:37	arg1	organization					39:50	fungal cell wall organization	22:50	fungal cell wall organization	22:50	A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR.					
34732740	3	43	theme	present	621:627	arg1	galactosaminogalactan					581:601	galactosaminogalactan	581:601	galactosaminogalactan	581:601	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	7	44	theme	wall	1101:1104	arg1	architecture					1106:1117	cell wall architecture	1096:1117	cell wall architecture	1096:1117	We propose a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses.					
34732740	4	45	theme	water	783:787	arg1	accessibility					789:801	water accessibility	783:801	water accessibility	783:801	Gene deletion reshuffles the composition and spatial organization of polysaccharides, with significant changes in their dynamics and water accessibility.					
34732740	4	46	theme	polysaccharides	719:733	arg1	composition					679:689	composition	679:689	composition	679:689	Gene deletion reshuffles the composition and spatial organization of polysaccharides, with significant changes in their dynamics and water accessibility.					
34732740	4	46	theme	polysaccharides	719:733	arg1	organization					703:714	spatial organization	695:714	spatial organization	695:714	Gene deletion reshuffles the composition and spatial organization of polysaccharides, with significant changes in their dynamics and water accessibility.					
34732740	7	47	theme	architecture	1106:1117	arg1	model					1087:1091	a revised model	1077:1091	a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses	1077:1213	We propose a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses.					
34732740	2	48	theme	wall	323:326	arg1	structure					328:336	the cell wall structure	314:336	the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides	314:441	Here we compare the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides.					
34732740	1	49	theme	supramolecular	199:212	arg1	architecture					214:225	the supramolecular architecture	195:225	the supramolecular architecture of this carbohydrate-rich composite	195:261	Vast efforts have been devoted to the development of antifungal drugs targeting the cell wall, but the supramolecular architecture of this carbohydrate-rich composite remains insufficiently understood.					
34732740	0	50	theme	functional	55:64	arg1	genomics					66:73	functional genomics	55:73	functional genomics	55:73	A molecular vision of fungal cell wall organization by functional genomics and solid-state NMR.					
34732740	5	51	from	distribution	808:819	arg1	isolated					851:858	isolated	851:858	isolated	851:858	The distribution of α-1,3-glucan in chemically isolated and dynamically distinct domains supports its functional diversity.					
34732740	5	51	from	distribution	808:819	arg1	domains					885:891	dynamically distinct domains	864:891	dynamically distinct domains	864:891	The distribution of α-1,3-glucan in chemically isolated and dynamically distinct domains supports its functional diversity.					
34732740	3	52	theme	mobile	636:641	arg1	phase					643:647	the mobile phase	632:647	the mobile phase	632:647	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	3	53	theme	intact	492:497	arg1	cells					499:503	intact cells	492:503	intact cells	492:503	High-resolution solid-state NMR spectroscopy of intact cells reveals a rigid core formed by chitin, β-1,3-glucan, and α-1,3-glucan, with galactosaminogalactan and galactomannan present in the mobile phase.					
34732740	2	54	theme	fumigatus	371:379	arg1	structure					328:336	the cell wall structure	314:336	the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides	314:441	Here we compare the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides.					
34732740	2	55	theme	mutants	390:396	arg1	structure					328:336	the cell wall structure	314:336	the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides	314:441	Here we compare the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides.					
34732740	4	56	theme	Gene	650:653	arg1	deletion					655:662	Gene deletion	650:662	Gene deletion	650:662	Gene deletion reshuffles the composition and spatial organization of polysaccharides, with significant changes in their dynamics and water accessibility.					
34732740	7	57	theme	revised	1079:1085	arg1	model					1087:1091	a revised model	1077:1091	a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses	1077:1213	We propose a revised model of cell wall architecture which will improve our understanding of the structural response of fungal pathogens to stresses.					
34732740	2	58	theme	Aspergillus	359:369	arg1	fumigatus					371:379	a fungal pathogen Aspergillus fumigatus	341:379	a fungal pathogen Aspergillus fumigatus	341:379	Here we compare the cell wall structure of a fungal pathogen Aspergillus fumigatus and four mutants depleted of major structural polysaccharides.					
32105695	8	0	theme	phagocytic	1317:1326	arg1	activity					1328:1335	the phagocytic activity	1313:1335	the phagocytic activity in macrophages	1313:1350	Above all, LLEP-P-І exerted greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages.					
32105695	1	1	theme	enzyme-assisted	171:185	arg1	extraction					187:196	enzyme-assisted extraction	171:196	enzyme-assisted extraction using α-amylase (LLEP-A), cellulose (LLEP-C), pectinase (LLEP-P) or protease (LLEP-PR)	171:283	Lotus leaf polysaccharides were extracted by enzyme-assisted extraction using α-amylase (LLEP-A), cellulose (LLEP-C), pectinase (LLEP-P) or protease (LLEP-PR).					
32105695	8	2	from	effects	1270:1276	arg1	activity					1328:1335	the phagocytic activity	1313:1335	the phagocytic activity in macrophages	1313:1350	Above all, LLEP-P-І exerted greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages.					
32105695	8	2	from	effects	1270:1276	arg1	production					1298:1307	NO and cytokines production	1281:1307	NO and cytokines production	1281:1307	Above all, LLEP-P-І exerted greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages.					
32105695	8	3	theme	cytokines	1288:1296	arg1	production					1298:1307	NO and cytokines production	1281:1307	NO and cytokines production	1281:1307	Above all, LLEP-P-І exerted greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages.					
32105695	4	4	theme	pectinase-assisted	735:752	arg1	extraction					754:763	pectinase-assisted extraction	735:763	pectinase-assisted extraction	735:763	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	10	5	theme	pectinase	1583:1591	arg1	application					1593:1603	pectinase application	1583:1603	pectinase application	1583:1603	Thus, these results suggest that pectinase application is most suitable to obtain immunostimulatory polysaccharides from lotus leaves.					
32105695	0	6	theme	leaf	104:107	arg1	polysaccharides					109:123	lotus leaf polysaccharides	98:123	lotus leaf polysaccharides	98:123	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	0	7	from	Effect	0:5	arg1	potential					85:93	bioactive potential	75:93	bioactive potential	75:93	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	0	7	from	Effect	0:5	arg1	properties					60:69	physicochemical properties	44:69	physicochemical properties	44:69	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	0	8	theme	lotus	98:102	arg1	polysaccharides					109:123	lotus leaf polysaccharides	98:123	lotus leaf polysaccharides	98:123	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	8	9	from	production	1298:1307	arg1	macrophages					1340:1350	macrophages	1340:1350	macrophages	1340:1350	Above all, LLEP-P-І exerted greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages.					
32105695	3	10	theme	chemical	521:528	arg1	compositions					530:541	chemical compositions	521:541	chemical compositions	521:541	HPAEC-PDA and HPSEC-RI profiles indicated that variations in their molecular weight patterns and chemical compositions.					
32105695	4	11	theme	cytokine	611:618	arg1	production					620:629	cytokine production	611:629	cytokine production	611:629	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	9	12	theme	JAK-STAT	1530:1537	arg1	signaling					1539:1547	JAK-STAT signaling	1530:1547	JAK-STAT signaling	1530:1547	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	0	13	theme	polysaccharides	109:123	arg1	potential					85:93	bioactive potential	75:93	bioactive potential	75:93	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	0	13	theme	polysaccharides	109:123	arg1	properties					60:69	physicochemical properties	44:69	physicochemical properties	44:69	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	5	14	theme	functional	945:954	arg1	properties					956:965	the structural and functional properties	926:965	the structural and functional properties	926:965	LLEP-P was further purified by gel filtration, and the main fraction (LLEP-P-І) was obtained to elucidate the structural and functional properties.					
32105695	7	15	theme	esterified	1167:1176	arg1	form					1178:1181	the predominant acidic and esterified form	1140:1181	the predominant acidic and esterified form	1140:1181	FT-IR spectra indicated the predominant acidic and esterified form, suggesting the pectic-like structure.					
32105695	4	16	from	activity	597:604	arg1	macrophages					634:644	macrophages	634:644	macrophages	634:644	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	9	17	theme	response	1454:1461	arg1	interferon					1507:1516	interferon	1507:1516	interferon via TLR and JAK-STAT signaling	1507:1547	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	9	17	theme	response	1454:1461	arg1	chemokines					1491:1500	chemokines	1491:1500	chemokines	1491:1500	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	9	17	theme	response	1454:1461	arg1	genes					1463:1467	macrophage immune response genes	1436:1467	macrophage immune response genes	1436:1467	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	9	17	theme	response	1454:1461	arg1	cytokines					1480:1488	cytokines	1480:1488	cytokines	1480:1488	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	7	18	theme	predominant	1144:1154	arg1	form					1178:1181	the predominant acidic and esterified form	1140:1181	the predominant acidic and esterified form	1140:1181	FT-IR spectra indicated the predominant acidic and esterified form, suggesting the pectic-like structure.					
32105695	2	19	theme	extracted	390:398	arg1	polysaccharides					400:414	hot-water extracted polysaccharides	380:414	hot-water extracted polysaccharides (LLWP)	380:421	Their physicochemical properties and immunostimulatory activities were compared with those of hot-water extracted polysaccharides (LLWP).					
32105695	2	19	theme	extracted	390:398	arg1	LLWP					417:420	LLWP	417:420	LLWP	417:420	Their physicochemical properties and immunostimulatory activities were compared with those of hot-water extracted polysaccharides (LLWP).					
32105695	8	20	theme	greater	1250:1256	arg1	effects					1270:1276	greater stimulation effects	1250:1276	greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages	1250:1350	Above all, LLEP-P-І exerted greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages.					
32105695	7	21	theme	acidic	1156:1161	arg1	form					1178:1181	the predominant acidic and esterified form	1140:1181	the predominant acidic and esterified form	1140:1181	FT-IR spectra indicated the predominant acidic and esterified form, suggesting the pectic-like structure.					
32105695	2	22	theme	physicochemical	292:306	arg1	properties					308:317	Their physicochemical properties	286:317	Their physicochemical properties	286:317	Their physicochemical properties and immunostimulatory activities were compared with those of hot-water extracted polysaccharides (LLWP).					
32105695	3	23	theme	HPSEC-RI	438:445	arg1	profiles					447:454	HPAEC-PDA and HPSEC-RI profiles	424:454	HPAEC-PDA and HPSEC-RI profiles	424:454	HPAEC-PDA and HPSEC-RI profiles indicated that variations in their molecular weight patterns and chemical compositions.					
32105695	0	24	theme	extraction	26:35	arg1	Effect					0:5	Effect	0:5	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.	0:124	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	9	25	theme	macrophage	1436:1445	arg1	interferon					1507:1516	interferon	1507:1516	interferon via TLR and JAK-STAT signaling	1507:1547	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	9	25	theme	macrophage	1436:1445	arg1	chemokines					1491:1500	chemokines	1491:1500	chemokines	1491:1500	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	9	25	theme	macrophage	1436:1445	arg1	genes					1463:1467	macrophage immune response genes	1436:1467	macrophage immune response genes	1436:1467	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	9	25	theme	macrophage	1436:1445	arg1	cytokines					1480:1488	cytokines	1480:1488	cytokines	1480:1488	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	4	26	theme	activation	808:817	arg1	enhancer					785:792	the most potent enhancer	769:792	the most potent enhancer of macrophage activation	769:817	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	4	26	theme	activation	808:817	arg1	LLEP-P					725:730	LLEP-P	725:730	LLEP-P by pectinase-assisted extraction	725:763	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	0	27	theme	enzyme-assisted	10:24	arg1	extraction					26:35	enzyme-assisted extraction	10:35	enzyme-assisted extraction	10:35	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	9	28	theme	immune	1447:1452	arg1	interferon					1507:1516	interferon	1507:1516	interferon via TLR and JAK-STAT signaling	1507:1547	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	9	28	theme	immune	1447:1452	arg1	chemokines					1491:1500	chemokines	1491:1500	chemokines	1491:1500	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	9	28	theme	immune	1447:1452	arg1	genes					1463:1467	macrophage immune response genes	1436:1467	macrophage immune response genes	1436:1467	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	9	28	theme	immune	1447:1452	arg1	cytokines					1480:1488	cytokines	1480:1488	cytokines	1480:1488	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	4	29	theme	macrophage	797:806	arg1	activation					808:817	macrophage activation	797:817	macrophage activation	797:817	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	6	30	theme	15.5:15.8:20.1:32.8	1095:1113	arg1	percentages					1080:1090	molar percentages	1074:1090	molar percentages of 15.5:15.8:20.1:32.8	1074:1113	LLEP-P-І (14.63 × 103 g/mol) mainly consisted of rhamnose, arabinose, galactose, and galacturonic acid at molar percentages of 15.5:15.8:20.1:32.8.					
32105695	4	31	theme	phagocytic	586:595	arg1	activity					597:604	phagocytic activity	586:604	phagocytic activity	586:604	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	7	32	theme	FT-IR	1116:1120	arg1	spectra					1122:1128	FT-IR spectra	1116:1128	FT-IR spectra	1116:1128	FT-IR spectra indicated the predominant acidic and esterified form, suggesting the pectic-like structure.					
32105695	0	33	dep	properties	60:69	arg1	the					40:42	the	40:42	the	40:42	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	2	34	theme	hot-water	380:388	arg1	polysaccharides					400:414	hot-water extracted polysaccharides	380:414	hot-water extracted polysaccharides (LLWP)	380:421	Their physicochemical properties and immunostimulatory activities were compared with those of hot-water extracted polysaccharides (LLWP).					
32105695	2	34	theme	hot-water	380:388	arg1	LLWP					417:420	LLWP	417:420	LLWP	417:420	Their physicochemical properties and immunostimulatory activities were compared with those of hot-water extracted polysaccharides (LLWP).					
32105695	4	35	theme	>	698:698	arg1	effects					560:566	their effects	554:566	their effects on proliferation, phagocytic activity, and cytokine production in macrophages	554:644	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	4	35	theme	>	698:698	arg1	LLEP-PR					700:706	LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR	666:706	LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR	666:706	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	3	36	theme	HPAEC-PDA	424:432	arg1	profiles					447:454	HPAEC-PDA and HPSEC-RI profiles	424:454	HPAEC-PDA and HPSEC-RI profiles	424:454	HPAEC-PDA and HPSEC-RI profiles indicated that variations in their molecular weight patterns and chemical compositions.					
32105695	4	37	from	effects	560:566	arg1	production					620:629	cytokine production	611:629	cytokine production	611:629	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	4	37	from	effects	560:566	arg1	proliferation					571:583	proliferation	571:583	proliferation	571:583	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	4	37	from	effects	560:566	arg1	activity					597:604	phagocytic activity	586:604	phagocytic activity	586:604	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	10	38	from	lotus	1671:1675	arg1	polysaccharides					1650:1664	immunostimulatory polysaccharides	1632:1664	immunostimulatory polysaccharides from lotus leaves	1632:1682	Thus, these results suggest that pectinase application is most suitable to obtain immunostimulatory polysaccharides from lotus leaves.					
32105695	8	39	from	activity	1328:1335	arg1	macrophages					1340:1350	macrophages	1340:1350	macrophages	1340:1350	Above all, LLEP-P-І exerted greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages.					
32105695	4	40	theme	LLEP-P > LLEP-C > LLEP-A > LLWP	666:696	arg1	effects					560:566	their effects	554:566	their effects on proliferation, phagocytic activity, and cytokine production in macrophages	554:644	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	4	40	theme	LLEP-P > LLEP-C > LLEP-A > LLWP	666:696	arg1	LLEP-PR					700:706	LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR	666:706	LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR	666:706	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	5	41	theme	gel	851:853	arg1	filtration					855:864	gel filtration	851:864	gel filtration	851:864	LLEP-P was further purified by gel filtration, and the main fraction (LLEP-P-І) was obtained to elucidate the structural and functional properties.					
32105695	4	42	from	production	620:629	arg1	macrophages					634:644	macrophages	634:644	macrophages	634:644	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	5	43	theme	main	875:878	arg1	LLEP-P-І					890:897	LLEP-P-І	890:897	LLEP-P-І	890:897	LLEP-P was further purified by gel filtration, and the main fraction (LLEP-P-І) was obtained to elucidate the structural and functional properties.					
32105695	5	43	theme	main	875:878	arg1	fraction					880:887	the main fraction	871:887	the main fraction (LLEP-P-І)	871:898	LLEP-P was further purified by gel filtration, and the main fraction (LLEP-P-І) was obtained to elucidate the structural and functional properties.					
32105695	3	44	from	variations	471:480	arg1	patterns					508:515	their molecular weight patterns	485:515	their molecular weight patterns	485:515	HPAEC-PDA and HPSEC-RI profiles indicated that variations in their molecular weight patterns and chemical compositions.					
32105695	3	44	from	variations	471:480	arg1	compositions					530:541	chemical compositions	521:541	chemical compositions	521:541	HPAEC-PDA and HPSEC-RI profiles indicated that variations in their molecular weight patterns and chemical compositions.					
32105695	1	45	theme	Lotus	126:130	arg1	polysaccharides					137:151	Lotus leaf polysaccharides	126:151	Lotus leaf polysaccharides	126:151	Lotus leaf polysaccharides were extracted by enzyme-assisted extraction using α-amylase (LLEP-A), cellulose (LLEP-C), pectinase (LLEP-P) or protease (LLEP-PR).					
32105695	0	46	theme	physicochemical	44:58	arg1	properties					60:69	physicochemical properties	44:69	physicochemical properties	44:69	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	3	47	dep	indicated	456:464	arg1	variations					471:480	variations	471:480	variations in their molecular weight patterns and chemical compositions	471:541	HPAEC-PDA and HPSEC-RI profiles indicated that variations in their molecular weight patterns and chemical compositions.					
32105695	8	48	theme	NO	1281:1282	arg1	production					1298:1307	NO and cytokines production	1281:1307	NO and cytokines production	1281:1307	Above all, LLEP-P-І exerted greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages.					
32105695	1	49	theme	leaf	132:135	arg1	polysaccharides					137:151	Lotus leaf polysaccharides	126:151	Lotus leaf polysaccharides	126:151	Lotus leaf polysaccharides were extracted by enzyme-assisted extraction using α-amylase (LLEP-A), cellulose (LLEP-C), pectinase (LLEP-P) or protease (LLEP-PR).					
32105695	8	50	theme	stimulation	1258:1268	arg1	effects					1270:1276	greater stimulation effects	1250:1276	greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages	1250:1350	Above all, LLEP-P-І exerted greater stimulation effects on NO and cytokines production and the phagocytic activity in macrophages.					
32105695	10	51	dep	lotus	1671:1675	arg1	leaves					1677:1682	leaves	1677:1682	leaves	1677:1682	Thus, these results suggest that pectinase application is most suitable to obtain immunostimulatory polysaccharides from lotus leaves.					
32105695	2	52	theme	immunostimulatory	323:339	arg1	activities					341:350	immunostimulatory activities	323:350	immunostimulatory activities	323:350	Their physicochemical properties and immunostimulatory activities were compared with those of hot-water extracted polysaccharides (LLWP).					
32105695	6	53	theme	molar	1074:1078	arg1	percentages					1080:1090	molar percentages	1074:1090	molar percentages of 15.5:15.8:20.1:32.8	1074:1113	LLEP-P-І (14.63 × 103 g/mol) mainly consisted of rhamnose, arabinose, galactose, and galacturonic acid at molar percentages of 15.5:15.8:20.1:32.8.					
32105695	4	54	theme	potent	778:783	arg1	enhancer					785:792	the most potent enhancer	769:792	the most potent enhancer of macrophage activation	769:817	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	4	54	theme	potent	778:783	arg1	LLEP-P					725:730	LLEP-P	725:730	LLEP-P by pectinase-assisted extraction	725:763	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	3	55	theme	weight	501:506	arg1	patterns					508:515	their molecular weight patterns	485:515	their molecular weight patterns	485:515	HPAEC-PDA and HPSEC-RI profiles indicated that variations in their molecular weight patterns and chemical compositions.					
32105695	3	56	theme	molecular	491:499	arg1	patterns					508:515	their molecular weight patterns	485:515	their molecular weight patterns	485:515	HPAEC-PDA and HPSEC-RI profiles indicated that variations in their molecular weight patterns and chemical compositions.					
32105695	0	57	theme	bioactive	75:83	arg1	potential					85:93	bioactive potential	75:93	bioactive potential	75:93	Effect of enzyme-assisted extraction on the physicochemical properties and bioactive potential of lotus leaf polysaccharides.					
32105695	9	58	theme	Transcriptome	1353:1365	arg1	analysis					1367:1374	Transcriptome analysis	1353:1374	Transcriptome analysis	1353:1374	Transcriptome analysis also demonstrated that LLEP-P and LLEP-P-І could upregulate macrophage immune response genes, including cytokines, chemokines, and interferon via TLR and JAK-STAT signaling.					
32105695	10	59	theme	immunostimulatory	1632:1648	arg1	polysaccharides					1650:1664	immunostimulatory polysaccharides	1632:1664	immunostimulatory polysaccharides from lotus leaves	1632:1682	Thus, these results suggest that pectinase application is most suitable to obtain immunostimulatory polysaccharides from lotus leaves.					
32105695	7	60	theme	pectic-like	1199:1209	arg1	structure					1211:1219	the pectic-like structure	1195:1219	the pectic-like structure	1195:1219	FT-IR spectra indicated the predominant acidic and esterified form, suggesting the pectic-like structure.					
32105695	4	61	from	proliferation	571:583	arg1	macrophages					634:644	macrophages	634:644	macrophages	634:644	Moreover, their effects on proliferation, phagocytic activity, and cytokine production in macrophages could be ordered as LLEP-P > LLEP-C > LLEP-A > LLWP > LLEP-PR, suggesting that LLEP-P by pectinase-assisted extraction was the most potent enhancer of macrophage activation.					
32105695	5	62	theme	structural	930:939	arg1	properties					956:965	the structural and functional properties	926:965	the structural and functional properties	926:965	LLEP-P was further purified by gel filtration, and the main fraction (LLEP-P-І) was obtained to elucidate the structural and functional properties.					
32105695	6	63	theme	galacturonic	1053:1064	arg1	acid					1066:1069	galacturonic acid	1053:1069	galacturonic acid	1053:1069	LLEP-P-І (14.63 × 103 g/mol) mainly consisted of rhamnose, arabinose, galactose, and galacturonic acid at molar percentages of 15.5:15.8:20.1:32.8.					
32530009	6	0	theme	physicochemical	1452:1466	arg1	environment					1468:1478	the fibril physicochemical environment	1441:1478	the fibril physicochemical environment	1441:1478	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	2	1	attach	present	358:364	arg2	groups					351:356	known fluorescent chemical groups	324:356	known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity	324:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	2	1	attach	present	358:364	arg1	structure					383:391	the molecular structure	369:391	the molecular structure of polysaccharides and the weak emission intensity	369:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	2	2	theme	chemical	342:349	arg1	groups					351:356	known fluorescent chemical groups	324:356	known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity	324:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	0	3	theme	nanofibrils	86:96	arg1	aggregation					46:56	aggregation	46:56	aggregation	46:56	Employing photoluminescence to rapidly follow aggregation and dispersion of cellulose nanofibrils.					
32530009	0	3	theme	nanofibrils	86:96	arg1	dispersion					62:71	dispersion	62:71	dispersion	62:71	Employing photoluminescence to rapidly follow aggregation and dispersion of cellulose nanofibrils.					
32530009	3	4	theme	molecular	681:689	arg1	forces					691:696	the molecular forces	677:696	the molecular forces between the polysaccharide chains	677:730	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	6	5	theme	pH	1375:1376	arg1	range					1378:1382	the examined pH range	1362:1382	the examined pH range	1362:1382	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	2	6	theme	fluorescent	330:340	arg1	groups					351:356	known fluorescent chemical groups	324:356	known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity	324:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	3	7	from	present	639:645	arg1	groups					655:660	oxyl groups	650:660	oxyl groups	650:660	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	6	8	theme	apparent	1289:1296	arg1	values					1302:1307	the apparent pKa values	1285:1307	the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment	1285:1478	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	4	9	theme	resultant	902:910	arg1	spectra					924:930	the resultant fluorescent spectra	898:930	the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy	898:987	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	6	10	theme	pKa	1298:1300	arg1	values					1302:1307	the apparent pKa values	1285:1307	the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment	1285:1478	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	6	11	from	changes	1430:1436	arg1	environment					1468:1478	the fibril physicochemical environment	1441:1478	the fibril physicochemical environment	1441:1478	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	6	12	theme	aggregation	1254:1264	arg1	concentrations					1266:1279	critical aggregation concentrations	1245:1279	critical aggregation concentrations	1245:1279	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	4	13	theme	cellulose	851:859	arg1	suspensions					872:882	oxidised cellulose nanofibril suspensions	842:882	oxidised cellulose nanofibril suspensions	842:882	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	3	14	theme	observed	526:533	arg1	due					564:566	due	564:566	due	564:566	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	3	14	theme	observed	526:533	arg1	luminescence					535:546	the observed luminescence	522:546	the observed luminescence	522:546	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	3	15	from	groups	655:660	arg1	present					639:645	present	639:645	present	639:645	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	3	16	theme	polysaccharide	710:723	arg1	chains					725:730	the polysaccharide chains	706:730	the polysaccharide chains	706:730	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	4	17	theme	oxidised	842:849	arg1	suspensions					872:882	oxidised cellulose nanofibril suspensions	842:882	oxidised cellulose nanofibril suspensions	842:882	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	3	18	contain	containing	608:617	arg2	pairs					633:637	lone electron pairs	619:637	lone electron pairs present in oxyl groups	619:660	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	3	18	contain	containing	608:617	arg1	orbitals					599:606	the n orbitals	593:606	the n orbitals	593:606	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	2	19	theme	molecular	373:381	arg1	structure					383:391	the molecular structure	369:391	the molecular structure of polysaccharides and the weak emission intensity	369:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	6	20	theme	fibril	1445:1450	arg1	environment					1468:1478	the fibril physicochemical environment	1441:1478	the fibril physicochemical environment	1441:1478	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	4	21	theme	suspensions	872:882	arg1	environment					804:814	the physicochemical environment	784:814	the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions	784:882	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	4	22	dep	environment	804:814	arg1	pH					835:836	pH	835:836	pH	835:836	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	4	22	dep	environment	804:814	arg1	concentration					817:829	concentration	817:829	concentration	817:829	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	2	23	theme	intensity	434:442	arg1	structure					383:391	the molecular structure	369:391	the molecular structure of polysaccharides and the weak emission intensity	369:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	4	24	theme	nanofibril	861:870	arg1	suspensions					872:882	oxidised cellulose nanofibril suspensions	842:882	oxidised cellulose nanofibril suspensions	842:882	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	2	25	from	present	358:364	arg1	structure					383:391	the molecular structure	369:391	the molecular structure of polysaccharides and the weak emission intensity	369:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	2	26	theme	emission	425:432	arg1	intensity					434:442	the weak emission intensity	416:442	the weak emission intensity	416:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	4	27	theme	laser	961:965	arg1	spectroscopy					976:987	multi-channel confocal laser scanning spectroscopy	938:987	multi-channel confocal laser scanning spectroscopy	938:987	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	6	28	theme	rapid	1424:1428	arg1	changes					1430:1436	rapid changes	1424:1436	rapid changes in the fibril physicochemical environment	1424:1478	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	1	29	theme	cellulose	120:128	arg1	polysaccharides					141:155	other polysaccharides	135:155	other polysaccharides	135:155	Photoluminescence of cellulose, and other polysaccharides, has long been presumed to be due to contamination of the material by other autofluorescent compounds - such as lignin, or proteins.					
32530009	1	29	theme	cellulose	120:128	arg1	Photoluminescence					99:115	Photoluminescence	99:115	Photoluminescence of cellulose	99:128	Photoluminescence of cellulose, and other polysaccharides, has long been presumed to be due to contamination of the material by other autofluorescent compounds - such as lignin, or proteins.					
32530009	6	30	theme	groups	1321:1326	arg1	determination					1228:1240	the determination	1224:1240	the determination of critical aggregation concentrations	1224:1279	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	6	30	theme	groups	1321:1326	arg1	values					1302:1307	the apparent pKa values	1285:1307	the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment	1285:1478	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	2	31	theme	weak	420:423	arg1	intensity					434:442	the weak emission intensity	416:442	the weak emission intensity	416:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	6	32	theme	concentrations	1266:1279	arg1	determination					1228:1240	the determination	1224:1240	the determination of critical aggregation concentrations	1224:1279	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	6	32	theme	concentrations	1266:1279	arg1	values					1302:1307	the apparent pKa values	1285:1307	the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment	1285:1478	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	4	33	theme	confocal	952:959	arg1	spectroscopy					976:987	multi-channel confocal laser scanning spectroscopy	938:987	multi-channel confocal laser scanning spectroscopy	938:987	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	3	34	theme	oxyl	650:653	arg1	groups					655:660	oxyl groups	650:660	oxyl groups	650:660	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	3	35	theme	recent	492:497	arg1	research					499:506	recent research	492:506	recent research	492:506	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	6	36	theme	technique	1405:1413	arg1	use					1394:1396	use	1394:1396	use of the technique to track rapid changes in the fibril physicochemical environment	1394:1478	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	1	37	dep	compounds	249:257	arg1	proteins					280:287	proteins	280:287	proteins	280:287	Photoluminescence of cellulose, and other polysaccharides, has long been presumed to be due to contamination of the material by other autofluorescent compounds - such as lignin, or proteins.					
32530009	1	37	dep	compounds	249:257	arg1	such					261:264	such	261:264	such	261:264	Photoluminescence of cellulose, and other polysaccharides, has long been presumed to be due to contamination of the material by other autofluorescent compounds - such as lignin, or proteins.					
32530009	3	38	theme	n	597:597	arg1	orbitals					599:606	the n orbitals	593:606	the n orbitals	593:606	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	2	39	theme	polysaccharides	396:410	arg1	structure					383:391	the molecular structure	369:391	the molecular structure of polysaccharides and the weak emission intensity	369:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	2	40	theme	typical	461:467	arg1	fluorophores					469:480	typical fluorophores	461:480	typical fluorophores	461:480	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	4	41	theme	scanning	967:974	arg1	spectroscopy					976:987	multi-channel confocal laser scanning spectroscopy	938:987	multi-channel confocal laser scanning spectroscopy	938:987	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	3	42	theme	present	639:645	arg1	pairs					633:637	lone electron pairs	619:637	lone electron pairs present in oxyl groups	619:660	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	6	43	theme	hydroxyl	1312:1319	arg1	groups					1321:1326	hydroxyl groups	1312:1326	hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment	1312:1478	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	1	44	theme	material	215:222	arg1	contamination					194:206	contamination	194:206	contamination of the material by other autofluorescent compounds - such as lignin, or proteins	194:287	Photoluminescence of cellulose, and other polysaccharides, has long been presumed to be due to contamination of the material by other autofluorescent compounds - such as lignin, or proteins.					
32530009	5	45	theme	current	1150:1156	arg1	theories					1158:1165	current theories	1150:1165	current theories	1150:1165	We confirm that both factors affect the material photoluminescence, specifically changing the intensity ratio between two localised emission maxima, supporting current theories.					
32530009	5	46	theme	intensity	1084:1092	arg1	ratio					1094:1098	the intensity ratio	1080:1098	the intensity ratio between two localised emission maxima	1080:1136	We confirm that both factors affect the material photoluminescence, specifically changing the intensity ratio between two localised emission maxima, supporting current theories.					
32530009	2	47	theme	known	324:328	arg1	groups					351:356	known fluorescent chemical groups	324:356	known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity	324:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	3	48	attach	present	639:645	arg2	pairs					633:637	lone electron pairs	619:637	lone electron pairs present in oxyl groups	619:660	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	3	48	attach	present	639:645	arg1	groups					655:660	oxyl groups	650:660	oxyl groups	650:660	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	4	49	theme	fluorescent	912:922	arg1	spectra					924:930	the resultant fluorescent spectra	898:930	the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy	898:987	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	2	50	from	structure	383:391	arg1	present					358:364	present	358:364	present	358:364	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	1	51	theme	other	135:139	arg1	polysaccharides					141:155	other polysaccharides	135:155	other polysaccharides	135:155	Photoluminescence of cellulose, and other polysaccharides, has long been presumed to be due to contamination of the material by other autofluorescent compounds - such as lignin, or proteins.					
32530009	4	52	theme	multi-channel	938:950	arg1	spectroscopy					976:987	multi-channel confocal laser scanning spectroscopy	938:987	multi-channel confocal laser scanning spectroscopy	938:987	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	5	53	theme	material	1030:1037	arg1	photoluminescence					1039:1055	the material photoluminescence	1026:1055	the material photoluminescence	1026:1055	We confirm that both factors affect the material photoluminescence, specifically changing the intensity ratio between two localised emission maxima, supporting current theories.					
32530009	3	54	theme	electron	624:631	arg1	pairs					633:637	lone electron pairs	619:637	lone electron pairs present in oxyl groups	619:660	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	5	55	theme	localised	1112:1120	arg1	maxima					1131:1136	two localised emission maxima	1108:1136	two localised emission maxima	1108:1136	We confirm that both factors affect the material photoluminescence, specifically changing the intensity ratio between two localised emission maxima, supporting current theories.					
32530009	4	56	theme	physicochemical	788:802	arg1	environment					804:814	the physicochemical environment	784:814	the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions	784:882	Here we investigate this theory further by varying the physicochemical environment (concentration and pH) of oxidised cellulose nanofibril suspensions and observing the resultant fluorescent spectra using multi-channel confocal laser scanning spectroscopy.					
32530009	1	57	theme	other	227:231	arg1	compounds					249:257	other autofluorescent compounds	227:257	other autofluorescent compounds - such as lignin, or proteins	227:287	Photoluminescence of cellulose, and other polysaccharides, has long been presumed to be due to contamination of the material by other autofluorescent compounds - such as lignin, or proteins.					
32530009	0	58	theme	cellulose	76:84	arg1	nanofibrils					86:96	cellulose nanofibrils	76:96	cellulose nanofibrils	76:96	Employing photoluminescence to rapidly follow aggregation and dispersion of cellulose nanofibrils.					
32530009	5	59	theme	emission	1122:1129	arg1	maxima					1131:1136	two localised emission maxima	1108:1136	two localised emission maxima	1108:1136	We confirm that both factors affect the material photoluminescence, specifically changing the intensity ratio between two localised emission maxima, supporting current theories.					
32530009	2	60	theme	present	358:364	arg1	groups					351:356	known fluorescent chemical groups	324:356	known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity	324:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	6	61	theme	critical	1245:1252	arg1	concentrations					1266:1279	critical aggregation concentrations	1245:1279	critical aggregation concentrations	1245:1279	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32530009	1	62	theme	autofluorescent	233:247	arg1	compounds					249:257	other autofluorescent compounds	227:257	other autofluorescent compounds - such as lignin, or proteins	227:287	Photoluminescence of cellulose, and other polysaccharides, has long been presumed to be due to contamination of the material by other autofluorescent compounds - such as lignin, or proteins.					
32530009	3	63	theme	lone	619:622	arg1	pairs					633:637	lone electron pairs	619:637	lone electron pairs present in oxyl groups	619:660	However, recent research suggests that the observed luminescence may actually be due to transitions involving the n orbitals containing lone electron pairs present in oxyl groups, stabilised by the molecular forces between the polysaccharide chains.					
32530009	2	64	theme	groups	351:356	arg1	lack					316:319	the lack	312:319	the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity	312:442	This is attributed to the lack of known fluorescent chemical groups present in the molecular structure of polysaccharides and the weak emission intensity when compared to typical fluorophores.					
32530009	6	65	theme	examined	1366:1373	arg1	range					1378:1382	the examined pH range	1362:1382	the examined pH range	1362:1382	Furthermore, we demonstrate that this variation enables the determination of critical aggregation concentrations and the apparent pKa values of hydroxyl groups that undergo deprotonation within the examined pH range, enabling use of the technique to track rapid changes in the fibril physicochemical environment.					
32644770	8	0	theme	material	895:902	arg1	bioactivity					876:886	The bioactivity	872:886	The bioactivity of the material	872:902	The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct.					
32644770	5	1	theme	BH	564:565	arg1	composite					567:575	The BH composite	560:575	The BH composite	560:575	The BH composite was characterized by a morphological method and physicochemical aspect.					
32644770	1	2	theme	chondral	243:250	arg1	lesions					259:265	degenerative chondral and OC lesions	230:265	degenerative chondral and OC lesions	230:265	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	11	3	theme	stable	1331:1336	arg1	properties					1354:1363	stable physicochemical properties	1331:1363	stable physicochemical properties	1331:1363	The data displayed stable physicochemical properties and mechanical characters when the DBPs were incorporated with a proper amount.					
32644770	10	4	theme	BH	1297:1298	arg1	composites					1300:1309	BH composites	1297:1309	BH composites	1297:1309	An in vivo study was performed to analyze the biocompatibility of the material and regeneration of the injured OC region implanted with BH composites.					
32644770	3	5	theme	new	456:458	arg1	hydrogel					350:357	a bilayer hydrogel	340:357	a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs)	340:436	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	3	5	theme	new	456:458	arg1	model					460:464	a new model	454:464	a new model	454:464	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	7	6	theme	composite	786:794	arg1	property					770:777	The thermodynamic property	752:777	The thermodynamic property of the composite	752:794	The thermodynamic property of the composite was determined to analyze thermal stability and interaction among matrices.					
32644770	1	7	theme	OC	256:257	arg1	lesions					259:265	degenerative chondral and OC lesions	230:265	degenerative chondral and OC lesions	230:265	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	9	8	theme	biochemical	1108:1118	arg1	characters					1120:1129	biochemical characters	1108:1129	biochemical characters	1108:1129	In vitro studies were carried out to study the viability and biochemical characters of the developed biomaterial.					
32644770	14	9	theme	BH	1818:1819	arg1	model					1821:1825	the BH model	1814:1825	the BH model	1814:1825	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	8	10	theme	body	938:941	arg1	fluid					943:947	simulated body fluid	928:947	simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct	928:1044	The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct.					
32644770	8	10	theme	body	938:941	arg1	SBF					950:952	SBF	950:952	SBF	950:952	The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct.					
32644770	2	11	theme	proper	289:294	arg1	model					296:300	a proper model	287:300	a proper model for OC TE	287:310	However, advancing a proper model for OC TE is still under way.					
32644770	7	12	theme	thermodynamic	756:768	arg1	property					770:777	The thermodynamic property	752:777	The thermodynamic property of the composite	752:794	The thermodynamic property of the composite was determined to analyze thermal stability and interaction among matrices.					
32644770	0	13	theme	Tissue	120:125	arg1	Engineering					127:137	Osteochondral Tissue Engineering	106:137	Osteochondral Tissue Engineering	106:137	Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering.					
32644770	9	14	theme	developed	1138:1146	arg1	biomaterial					1148:1158	the developed biomaterial	1134:1158	the developed biomaterial	1134:1158	In vitro studies were carried out to study the viability and biochemical characters of the developed biomaterial.					
32644770	14	15	theme	bone	1800:1803	arg1	layer					1805:1809	the bone layer	1796:1809	the bone layer of the BH model	1796:1825	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	16	16	theme	OC	2068:2069	arg1	regeneration					2071:2082	OC regeneration	2068:2082	OC regeneration	2068:2082	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	14	17	theme	DBP-loaded	1779:1788	arg1	GG					1790:1791	DBP-loaded GG	1779:1791	DBP-loaded GG	1779:1791	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	9	18	dep	viability	1094:1102	arg1	the					1090:1092	the	1090:1092	the	1090:1092	In vitro studies were carried out to study the viability and biochemical characters of the developed biomaterial.					
32644770	12	19	theme	apatite	1516:1522	arg1	formation					1524:1532	apatite formation	1516:1532	apatite formation	1516:1532	The bioactivity of the DBP-loaded hydrogels displayed a high amount of apatite formation.					
32644770	5	20	theme	morphological	600:612	arg1	method					614:619	a morphological method	598:619	a morphological method	598:619	The BH composite was characterized by a morphological method and physicochemical aspect.					
32644770	1	21	theme	Osteochondral	140:152	arg1	TE					179:180	TE	179:180	TE	179:180	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	1	21	theme	Osteochondral	140:152	arg1	engineering					166:176	Osteochondral (OC) tissue engineering	140:176	Osteochondral (OC) tissue engineering (TE)	140:181	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	1	21	theme	Osteochondral	140:152	arg1	strategy					198:205	a promising strategy	186:205	a promising strategy to regenerate acute or degenerative chondral and OC lesions	186:265	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	12	22	theme	hydrogels	1479:1487	arg1	bioactivity					1449:1459	The bioactivity	1445:1459	The bioactivity of the DBP-loaded hydrogels	1445:1487	The bioactivity of the DBP-loaded hydrogels displayed a high amount of apatite formation.					
32644770	0	23	theme	Gellan	58:63	arg1	Gum					65:67	Gellan Gum	58:67	Gellan Gum	58:67	Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering.					
32644770	1	24	theme	OC	155:156	arg1	TE					179:180	TE	179:180	TE	179:180	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	1	24	theme	OC	155:156	arg1	engineering					166:176	Osteochondral (OC) tissue engineering	140:176	Osteochondral (OC) tissue engineering (TE)	140:181	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	1	24	theme	OC	155:156	arg1	strategy					198:205	a promising strategy	186:205	a promising strategy to regenerate acute or degenerative chondral and OC lesions	186:265	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	8	25	from	formation	991:999	arg1	construct					1036:1044	the BH construct	1029:1044	the BH construct	1029:1044	The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct.					
32644770	1	26	theme	tissue	159:164	arg1	TE					179:180	TE	179:180	TE	179:180	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	1	26	theme	tissue	159:164	arg1	engineering					166:176	Osteochondral (OC) tissue engineering	140:176	Osteochondral (OC) tissue engineering (TE)	140:181	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	1	26	theme	tissue	159:164	arg1	strategy					198:205	a promising strategy	186:205	a promising strategy to regenerate acute or degenerative chondral and OC lesions	186:265	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	10	27	theme	in	1164:1165	arg1	study					1172:1176	An in vivo study	1161:1176	An in vivo study	1161:1176	An in vivo study was performed to analyze the biocompatibility of the material and regeneration of the injured OC region implanted with BH composites.					
32644770	0	28	theme	Bone	87:90	arg1	Particles					92:100	Gellan Gum and Demineralized Bone Particles	58:100	Gellan Gum and Demineralized Bone Particles	58:100	Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering.					
32644770	15	29	theme	long-term	1832:1840	arg1	study					1850:1854	The long-term in vivo study	1828:1854	The long-term in vivo study	1828:1854	The long-term in vivo study displayed excellent biocompatibility and great potential in the OC defected region.					
32644770	11	30	theme	mechanical	1369:1378	arg1	characters					1380:1389	mechanical characters	1369:1389	mechanical characters	1369:1389	The data displayed stable physicochemical properties and mechanical characters when the DBPs were incorporated with a proper amount.					
32644770	3	31	theme	gellan	373:378	arg1	hydrogel					389:396	gellan gum (GG) hydrogel	373:396	gellan gum (GG) hydrogel	373:396	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	14	32	theme	biochemical	1639:1649	arg1	studies					1651:1657	The biochemical studies	1635:1657	The biochemical studies	1635:1657	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	6	33	from	applicability	728:740	arg1	TE					748:749	OC TE	745:749	OC TE	745:749	The mechanical and rheological characters were further confirmed to verify its applicability in OC TE.					
32644770	16	34	theme	promising	2135:2143	arg1	model					2096:2100	the BH model	2089:2100	the BH model suggested in this study	2089:2124	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	16	34	theme	promising	2135:2143	arg1	model					2157:2161	a promising biomaterial model	2133:2161	a promising biomaterial model for OC TE	2133:2171	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	14	35	theme	expression	1730:1739	arg1	level					1676:1680	a high level	1669:1680	a high level	1669:1680	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	15	36	theme	OC	1920:1921	arg1	region					1932:1937	the OC defected region	1916:1937	the OC defected region	1916:1937	The long-term in vivo study displayed excellent biocompatibility and great potential in the OC defected region.					
32644770	16	37	theme	OC	2167:2168	arg1	TE					2170:2171	OC TE	2167:2171	OC TE	2167:2171	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	8	38	theme	crystalline	1004:1014	arg1	structure					1016:1024	crystalline structure	1004:1024	crystalline structure	1004:1024	The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct.					
32644770	15	39	theme	great	1897:1901	arg1	potential					1903:1911	great potential	1897:1911	great potential	1897:1911	The long-term in vivo study displayed excellent biocompatibility and great potential in the OC defected region.					
32644770	9	40	theme	In	1047:1048	arg1	studies					1056:1062	In vitro studies	1047:1062	In vitro studies	1047:1062	In vitro studies were carried out to study the viability and biochemical characters of the developed biomaterial.					
32644770	3	41	theme	bone	416:419	arg1	DBPs					432:435	DBPs	432:435	DBPs	432:435	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	3	41	theme	bone	416:419	arg1	particles					421:429	gellan gum (GG) hydrogel and demineralized bone particles	373:429	gellan gum (GG) hydrogel and demineralized bone particles (DBPs)	373:436	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	0	42	theme	Hydrogel	46:53	arg1	Potential					21:29	Potential	21:29	Potential	21:29	Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering.					
32644770	0	42	theme	Hydrogel	46:53	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering.					
32644770	13	43	theme	material	1570:1577	arg1	cytotoxicity					1539:1550	The cytotoxicity	1535:1550	The cytotoxicity of the fabricated material	1535:1577	The cytotoxicity of the fabricated material was low, which allows application in vitro and in vivo.					
32644770	13	43	theme	material	1570:1577	arg1	low					1583:1585	low	1583:1585	low	1583:1585	The cytotoxicity of the fabricated material was low, which allows application in vitro and in vivo.					
32644770	6	44	theme	rheological	668:678	arg1	characters					680:689	The mechanical and rheological characters	649:689	The mechanical and rheological characters	649:689	The mechanical and rheological characters were further confirmed to verify its applicability in OC TE.					
32644770	2	45	theme	OC	306:307	arg1	TE					309:310	OC TE	306:310	OC TE	306:310	However, advancing a proper model for OC TE is still under way.					
32644770	14	46	from	application	1764:1774	arg1	layer					1805:1809	the bone layer	1796:1809	the bone layer of the BH model	1796:1825	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	14	47	theme	alkaline	1685:1692	arg1	ALP					1707:1709	ALP	1707:1709	ALP	1707:1709	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	14	47	theme	alkaline	1685:1692	arg1	phosphatase					1694:1704	alkaline phosphatase	1685:1704	alkaline phosphatase (ALP) activity	1685:1719	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	8	48	theme	BH	1033:1034	arg1	construct					1036:1044	the BH construct	1029:1044	the BH construct	1029:1044	The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct.					
32644770	16	49	theme	BH	2093:2094	arg1	model					2096:2100	the BH model	2089:2100	the BH model suggested in this study	2089:2124	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	16	49	theme	BH	2093:2094	arg1	model					2157:2161	a promising biomaterial model	2133:2161	a promising biomaterial model for OC TE	2133:2171	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	10	50	theme	region	1275:1280	arg1	regeneration					1244:1255	regeneration	1244:1255	regeneration of the injured OC region implanted with BH composites	1244:1309	An in vivo study was performed to analyze the biocompatibility of the material and regeneration of the injured OC region implanted with BH composites.					
32644770	10	50	theme	region	1275:1280	arg1	biocompatibility					1207:1222	the biocompatibility	1203:1222	the biocompatibility of the material	1203:1238	An in vivo study was performed to analyze the biocompatibility of the material and regeneration of the injured OC region implanted with BH composites.					
32644770	10	51	theme	injured	1264:1270	arg1	region					1275:1280	the injured OC region	1260:1280	the injured OC region implanted with BH composites	1260:1309	An in vivo study was performed to analyze the biocompatibility of the material and regeneration of the injured OC region implanted with BH composites.					
32644770	7	52	theme	thermal	822:828	arg1	stability					830:838	thermal stability	822:838	thermal stability	822:838	The thermodynamic property of the composite was determined to analyze thermal stability and interaction among matrices.					
32644770	1	53	theme	degenerative	230:241	arg1	lesions					259:265	degenerative chondral and OC lesions	230:265	degenerative chondral and OC lesions	230:265	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	6	54	theme	OC	745:746	arg1	TE					748:749	OC TE	745:749	OC TE	745:749	The mechanical and rheological characters were further confirmed to verify its applicability in OC TE.					
32644770	8	55	theme	simulated	928:936	arg1	fluid					943:947	simulated body fluid	928:947	simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct	928:1044	The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct.					
32644770	8	55	theme	simulated	928:936	arg1	SBF					950:952	SBF	950:952	SBF	950:952	The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct.					
32644770	14	56	theme	model	1821:1825	arg1	layer					1805:1809	the bone layer	1796:1809	the bone layer of the BH model	1796:1825	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	15	57	dep	in	1842:1843	arg1	vivo					1845:1848	vivo	1845:1848	vivo	1845:1848	The long-term in vivo study displayed excellent biocompatibility and great potential in the OC defected region.					
32644770	8	58	theme	fluid	943:947	arg1	solution					955:962	simulated body fluid (SBF) solution	928:962	simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct	928:1044	The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct.					
32644770	16	59	theme	therapeutic	2041:2051	arg1	strategies					2053:2062	the therapeutic strategies	2037:2062	the therapeutic strategies for OC regeneration	2037:2082	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	5	60	theme	physicochemical	625:639	arg1	aspect					641:646	physicochemical aspect	625:646	physicochemical aspect	625:646	The BH composite was characterized by a morphological method and physicochemical aspect.					
32644770	9	61	theme	biomaterial	1148:1158	arg1	viability					1094:1102	viability	1094:1102	viability	1094:1102	In vitro studies were carried out to study the viability and biochemical characters of the developed biomaterial.					
32644770	9	61	theme	biomaterial	1148:1158	arg1	characters					1120:1129	biochemical characters	1108:1129	biochemical characters	1108:1129	In vitro studies were carried out to study the viability and biochemical characters of the developed biomaterial.					
32644770	1	62	theme	promising	188:196	arg1	engineering					166:176	Osteochondral (OC) tissue engineering	140:176	Osteochondral (OC) tissue engineering (TE)	140:181	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	1	62	theme	promising	188:196	arg1	strategy					198:205	a promising strategy	186:205	a promising strategy to regenerate acute or degenerative chondral and OC lesions	186:265	Osteochondral (OC) tissue engineering (TE) is a promising strategy to regenerate acute or degenerative chondral and OC lesions.					
32644770	14	63	theme	GG	1790:1791	arg1	application					1764:1774	promising application	1754:1774	promising application of DBP-loaded GG in the bone layer of the BH model	1754:1825	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	12	64	theme	formation	1524:1532	arg1	amount					1506:1511	a high amount	1499:1511	a high amount of apatite formation	1499:1532	The bioactivity of the DBP-loaded hydrogels displayed a high amount of apatite formation.					
32644770	12	64	theme	formation	1524:1532	arg1	formation					1524:1532	apatite formation	1516:1532	apatite formation	1516:1532	The bioactivity of the DBP-loaded hydrogels displayed a high amount of apatite formation.					
32644770	0	65	theme	Gum	65:67	arg1	Particles					92:100	Gellan Gum and Demineralized Bone Particles	58:100	Gellan Gum and Demineralized Bone Particles	58:100	Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering.					
32644770	14	66	theme	promising	1754:1762	arg1	application					1764:1774	promising application	1754:1774	promising application of DBP-loaded GG in the bone layer of the BH model	1754:1825	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	12	67	theme	high	1501:1504	arg1	amount					1506:1511	a high amount	1499:1511	a high amount of apatite formation	1499:1532	The bioactivity of the DBP-loaded hydrogels displayed a high amount of apatite formation.					
32644770	12	67	theme	high	1501:1504	arg1	formation					1524:1532	apatite formation	1516:1532	apatite formation	1516:1532	The bioactivity of the DBP-loaded hydrogels displayed a high amount of apatite formation.					
32644770	0	68	theme	Demineralized	73:85	arg1	Bone					87:90	Demineralized Bone	73:90	Demineralized Bone	73:90	Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering.					
32644770	15	69	theme	excellent	1866:1874	arg1	biocompatibility					1876:1891	excellent biocompatibility	1866:1891	excellent biocompatibility	1866:1891	The long-term in vivo study displayed excellent biocompatibility and great potential in the OC defected region.					
32644770	11	70	theme	proper	1430:1435	arg1	amount					1437:1442	a proper amount	1428:1442	a proper amount	1428:1442	The data displayed stable physicochemical properties and mechanical characters when the DBPs were incorporated with a proper amount.					
32644770	0	71	theme	Particles	92:100	arg1	Hydrogel					46:53	a Bilayered Hydrogel	34:53	a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering	34:137	Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering.					
32644770	10	72	dep	in	1164:1165	arg1	vivo					1167:1170	vivo	1167:1170	vivo	1167:1170	An in vivo study was performed to analyze the biocompatibility of the material and regeneration of the injured OC region implanted with BH composites.					
32644770	12	73	theme	DBP-loaded	1468:1477	arg1	hydrogels					1479:1487	the DBP-loaded hydrogels	1464:1487	the DBP-loaded hydrogels	1464:1487	The bioactivity of the DBP-loaded hydrogels displayed a high amount of apatite formation.					
32644770	16	74	theme	combined	1991:1998	arg1	approaches					2015:2024	combined and innovative approaches	1991:2024	approaches	2015:2024	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	0	75	theme	Osteochondral	106:118	arg1	Engineering					127:137	Osteochondral Tissue Engineering	106:137	Osteochondral Tissue Engineering	106:137	Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering.					
32644770	15	76	theme	in	1842:1843	arg1	study					1850:1854	The long-term in vivo study	1828:1854	The long-term in vivo study	1828:1854	The long-term in vivo study displayed excellent biocompatibility and great potential in the OC defected region.					
32644770	11	77	theme	physicochemical	1338:1352	arg1	properties					1354:1363	stable physicochemical properties	1331:1363	stable physicochemical properties	1331:1363	The data displayed stable physicochemical properties and mechanical characters when the DBPs were incorporated with a proper amount.					
32644770	3	78	theme	gum	380:382	arg1	hydrogel					389:396	gellan gum (GG) hydrogel	373:396	gellan gum (GG) hydrogel	373:396	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	9	79	dep	In	1047:1048	arg1	vitro					1050:1054	vitro	1050:1054	vitro	1050:1054	In vitro studies were carried out to study the viability and biochemical characters of the developed biomaterial.					
32644770	3	80	theme	bilayer	342:348	arg1	BH					360:361	BH	360:361	BH	360:361	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	3	80	theme	bilayer	342:348	arg1	model					460:464	a new model	454:464	a new model	454:464	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	3	80	theme	bilayer	342:348	arg1	hydrogel					350:357	a bilayer hydrogel	340:357	a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs)	340:436	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	16	81	theme	approaches	2015:2024	arg1	significance					1975:1986	the significance	1971:1986	the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration	1971:2082	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	10	82	theme	material	1231:1238	arg1	regeneration					1244:1255	regeneration	1244:1255	regeneration of the injured OC region implanted with BH composites	1244:1309	An in vivo study was performed to analyze the biocompatibility of the material and regeneration of the injured OC region implanted with BH composites.					
32644770	10	82	theme	material	1231:1238	arg1	biocompatibility					1207:1222	the biocompatibility	1203:1222	the biocompatibility of the material	1203:1238	An in vivo study was performed to analyze the biocompatibility of the material and regeneration of the injured OC region implanted with BH composites.					
32644770	14	83	theme	gene	1725:1728	arg1	expression					1730:1739	gene expression	1725:1739	gene expression	1725:1739	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	6	84	theme	mechanical	653:662	arg1	characters					680:689	The mechanical and rheological characters	649:689	The mechanical and rheological characters	649:689	The mechanical and rheological characters were further confirmed to verify its applicability in OC TE.					
32644770	4	85	theme	BH	471:472	arg1	composite					474:482	The BH composite	467:482	The BH composite	467:482	The BH composite can be fabricated easily with a cell-friendly biomaterial and cross-linker.					
32644770	15	86	theme	defected	1923:1930	arg1	region					1932:1937	the OC defected region	1916:1937	the OC defected region	1916:1937	The long-term in vivo study displayed excellent biocompatibility and great potential in the OC defected region.					
32644770	3	87	theme	hydrogel	389:396	arg1	DBPs					432:435	DBPs	432:435	DBPs	432:435	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	3	87	theme	hydrogel	389:396	arg1	particles					421:429	gellan gum (GG) hydrogel and demineralized bone particles	373:429	gellan gum (GG) hydrogel and demineralized bone particles (DBPs)	373:436	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	0	88	theme	Bilayered	36:44	arg1	Hydrogel					46:53	a Bilayered Hydrogel	34:53	a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering	34:137	Characterization and Potential of a Bilayered Hydrogel of Gellan Gum and Demineralized Bone Particles for Osteochondral Tissue Engineering.					
32644770	14	89	theme	activity	1712:1719	arg1	level					1676:1680	a high level	1669:1680	a high level	1669:1680	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	8	90	theme	structure	1016:1024	arg1	formation					991:999	the formation	987:999	the formation of crystalline structure in the BH construct	987:1044	The bioactivity of the material was studied by treating simulated body fluid (SBF) solution for 28 days to examine the formation of crystalline structure in the BH construct.					
32644770	3	91	theme	GG	385:386	arg1	hydrogel					389:396	gellan gum (GG) hydrogel	373:396	gellan gum (GG) hydrogel	373:396	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	13	92	theme	fabricated	1559:1568	arg1	material					1570:1577	the fabricated material	1555:1577	the fabricated material	1555:1577	The cytotoxicity of the fabricated material was low, which allows application in vitro and in vivo.					
32644770	16	93	theme	innovative	2004:2013	arg1	approaches					2015:2024	combined and innovative approaches	1991:2024	approaches	2015:2024	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	14	94	theme	phosphatase	1694:1704	arg1	activity					1712:1719	alkaline phosphatase (ALP) activity	1685:1719	alkaline phosphatase (ALP) activity	1685:1719	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32644770	3	95	theme	demineralized	402:414	arg1	bone					416:419	demineralized bone	402:419	demineralized bone	402:419	Herein, a bilayer hydrogel (BH) based on gellan gum (GG) hydrogel and demineralized bone particles (DBPs) is suggested as a new model.					
32644770	16	96	theme	biomaterial	2145:2155	arg1	model					2096:2100	the BH model	2089:2100	the BH model suggested in this study	2089:2124	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	16	96	theme	biomaterial	2145:2155	arg1	model					2157:2161	a promising biomaterial model	2133:2161	a promising biomaterial model for OC TE	2133:2171	Overall, these results suggest the significance of combined and innovative approaches to improve the therapeutic strategies for OC regeneration, and the BH model suggested in this study can be a promising biomaterial model for OC TE.					
32644770	4	97	theme	cell-friendly	516:528	arg1	biomaterial					530:540	a cell-friendly biomaterial	514:540	a cell-friendly biomaterial	514:540	The BH composite can be fabricated easily with a cell-friendly biomaterial and cross-linker.					
32644770	10	98	theme	OC	1272:1273	arg1	region					1275:1280	the injured OC region	1260:1280	the injured OC region implanted with BH composites	1260:1309	An in vivo study was performed to analyze the biocompatibility of the material and regeneration of the injured OC region implanted with BH composites.					
32644770	14	99	theme	high	1671:1674	arg1	level					1676:1680	a high level	1669:1680	a high level	1669:1680	The biochemical studies displayed a high level of alkaline phosphatase (ALP) activity and gene expression, which shows promising application of DBP-loaded GG in the bone layer of the BH model.					
32810537	0	0	theme	polysaccharides	99:113	arg1	cells					90:94	RAW264.7 cells	81:94	RAW264.7 cells of polysaccharides	81:113	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	7	1	theme	molecular	993:1001	arg1	determination					1010:1022	molecular weight determination	993:1022	molecular weight determination	993:1022	UV-vis and FT-IR analysis combined with molecular weight determination further indicated that the five fractions were polydisperse polysaccharides.					
32810537	0	2	from	extraction	19:28	arg1	cells					90:94	RAW264.7 cells	81:94	RAW264.7 cells of polysaccharides	81:113	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	5	3	from	GluA	833:836	arg1	common					797:802	common	797:802	common	797:802	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	1	4	theme	Trichosanthes	211:223	arg1	kirilowii					225:233	Trichosanthes kirilowii Maxim seeds (TKMSP)	211:253	Trichosanthes kirilowii Maxim seeds (TKMSP)	211:253	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	5	5	from	Arab	860:863	arg1	common					797:802	common	797:802	common	797:802	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	2	6	theme	optimum	354:360	arg1	conditions					373:382	The optimum extraction conditions	350:382	The optimum extraction conditions	350:382	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	2	6	theme	optimum	354:360	arg1	follows					400:406	follows	400:406	follows	400:406	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	0	7	theme	Trichosanthes	120:132	arg1	kirilowii					134:142	Trichosanthes kirilowii	120:142	Trichosanthes kirilowii	120:142	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	7	8	theme	FT-IR	964:968	arg1	analysis					970:977	UV-vis and FT-IR analysis	953:977	analysis	970:977	UV-vis and FT-IR analysis combined with molecular weight determination further indicated that the five fractions were polydisperse polysaccharides.					
32810537	8	9	theme	significant	1103:1113	arg1	difference					1115:1124	A significant difference	1101:1124	A significant difference	1101:1124	A significant difference was achieved in the structural characterization of these five fractions.					
32810537	5	10	from	Rha	828:830	arg1	common					797:802	common	797:802	common	797:802	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	0	11	theme	Maxim	144:148	arg1	seeds					150:154	Maxim seeds	144:154	Maxim seeds	144:154	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	11	theme	Maxim	144:148	arg1	characterization					31:46	characterization	31:46	characterization	31:46	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	11	theme	Maxim	144:148	arg1	activity					69:76	immunomodulatory activity	52:76	immunomodulatory activity	52:76	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	11	theme	Maxim	144:148	arg1	extraction					19:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	12	from	activity	69:76	arg1	cells					90:94	RAW264.7 cells	81:94	RAW264.7 cells of polysaccharides	81:113	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	7	13	theme	UV-vis	953:958	arg1	analysis					970:977	UV-vis and FT-IR analysis	953:977	analysis	970:977	UV-vis and FT-IR analysis combined with molecular weight determination further indicated that the five fractions were polydisperse polysaccharides.					
32810537	8	14	theme	structural	1146:1155	arg1	characterization					1157:1172	the structural characterization	1142:1172	the structural characterization of these five fractions	1142:1196	A significant difference was achieved in the structural characterization of these five fractions.					
32810537	2	15	dep	follows	400:406	arg1	42 mL/g					428:434	liquid-solid ratio 42 mL/g	409:434	liquid-solid ratio 42 mL/g	409:434	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	3	16	with	consistent	599:608	arg1	value					637:641	the predicted value	623:641	the predicted value (2.44%)	623:649	Under this conditions, the mean value of TKMSP yield 2.43 ± 0.45% (n = 3), which was consistent closely with the predicted value (2.44%).					
32810537	3	16	with	consistent	599:608	arg1	%					648:648	2.44%	644:648	2.44%	644:648	Under this conditions, the mean value of TKMSP yield 2.43 ± 0.45% (n = 3), which was consistent closely with the predicted value (2.44%).					
32810537	5	17	from	Glu	845:847	arg1	common					797:802	common	797:802	common	797:802	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	2	18	theme	power	477:481	arg1	570 W					483:487	microwave power 570 W	467:487	microwave power 570 W	467:487	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	2	18	theme	power	477:481	arg1	follows					400:406	follows	400:406	follows	400:406	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	1	19	theme	Maxim	235:239	arg1	seeds					241:245	Maxim seeds	235:245	Trichosanthes kirilowii Maxim seeds (TKMSP)	211:253	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	1	19	theme	Maxim	235:239	arg1	TKMSP					248:252	TKMSP	248:252	TKMSP	248:252	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	1	20	from	extraction	176:185	arg1	kirilowii					225:233	Trichosanthes kirilowii Maxim seeds (TKMSP)	211:253	Trichosanthes kirilowii Maxim seeds (TKMSP)	211:253	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	9	21	theme	immunosuppressive	1215:1231	arg1	activity					1233:1240	immunosuppressive activity	1215:1240	immunosuppressive activity	1215:1240	TKMSP exhibited immunosuppressive activity on RAW264.7 cells.					
32810537	2	22	theme	liquid-solid	409:420	arg1	42 mL/g					428:434	liquid-solid ratio 42 mL/g	409:434	liquid-solid ratio 42 mL/g	409:434	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	0	23	theme	Microwave-assisted	0:17	arg1	seeds					150:154	Maxim seeds	144:154	Maxim seeds	144:154	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	23	theme	Microwave-assisted	0:17	arg1	extraction					19:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	9	24	theme	RAW264.7	1245:1252	arg1	cells					1254:1258	RAW264.7 cells	1245:1258	RAW264.7 cells	1245:1258	TKMSP exhibited immunosuppressive activity on RAW264.7 cells.					
32810537	5	25	from	GalA	839:842	arg1	common					797:802	common	797:802	common	797:802	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	10	26	theme	potential	1284:1292	arg1	agent					1312:1316	a potential immunosuppressant agent	1282:1316	a potential immunosuppressant agent in medicine	1282:1328	It can be applied as a potential immunosuppressant agent in medicine.					
32810537	10	26	theme	potential	1284:1292	arg1	It					1261:1262	It	1261:1262	It	1261:1262	It can be applied as a potential immunosuppressant agent in medicine.					
32810537	2	27	theme	microwave	467:475	arg1	570 W					483:487	microwave power 570 W	467:487	microwave power 570 W	467:487	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	2	27	theme	microwave	467:475	arg1	follows					400:406	follows	400:406	follows	400:406	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	1	28	theme	Central	318:324	arg1	CCD					344:346	CCD	344:346	CCD	344:346	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	1	28	theme	Central	318:324	arg1	design					336:341	Central composite design	318:341	Central composite design (CCD)	318:347	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	1	29	theme	composite	326:334	arg1	CCD					344:346	CCD	344:346	CCD	344:346	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	1	29	theme	composite	326:334	arg1	design					336:341	Central composite design	318:341	Central composite design (CCD)	318:347	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	0	30	dep	seeds	150:154	arg1	seeds					150:154	Maxim seeds	144:154	Maxim seeds	144:154	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	30	dep	seeds	150:154	arg1	characterization					31:46	characterization	31:46	characterization	31:46	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	30	dep	seeds	150:154	arg1	activity					69:76	immunomodulatory activity	52:76	immunomodulatory activity	52:76	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	30	dep	seeds	150:154	arg1	extraction					19:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	2	31	theme	temperature	448:458	arg1	follows					400:406	follows	400:406	follows	400:406	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	2	31	theme	temperature	448:458	arg1	80 °C					460:464	extraction temperature 80 °C	437:464	extraction temperature 80 °C	437:464	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	0	32	theme	immunomodulatory	52:67	arg1	seeds					150:154	Maxim seeds	144:154	Maxim seeds	144:154	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	32	theme	immunomodulatory	52:67	arg1	activity					69:76	immunomodulatory activity	52:76	immunomodulatory activity	52:76	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	1	33	dep	methodology	292:302	arg1	base					310:313	base	310:313	Response surface methodology (RSM) base	275:313	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	2	34	theme	extraction	437:446	arg1	follows					400:406	follows	400:406	follows	400:406	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	2	34	theme	extraction	437:446	arg1	80 °C					460:464	extraction temperature 80 °C	437:464	extraction temperature 80 °C	437:464	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	2	35	theme	time	501:504	arg1	follows					400:406	follows	400:406	follows	400:406	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	2	35	theme	time	501:504	arg1	26 min					506:511	extraction time 26 min	490:511	extraction time 26 min	490:511	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	4	36	dep	polysaccharides	661:675	arg1	TKMSP-3					696:702	TKMSP-3	696:702	TKMSP-3	696:702	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	36	dep	polysaccharides	661:675	arg1	polysaccharides					661:675	The five polysaccharides	652:675	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5)	652:724	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	36	dep	polysaccharides	661:675	arg1	TKMSP-1					678:684	TKMSP-1	678:684	TKMSP-1	678:684	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	36	dep	polysaccharides	661:675	arg1	TKMSP-4					705:711	TKMSP-4	705:711	TKMSP-4	705:711	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	36	dep	polysaccharides	661:675	arg1	TKMSP-5					717:723	TKMSP-5	717:723	TKMSP-5	717:723	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	36	dep	polysaccharides	661:675	arg1	TKMSP-2					687:693	TKMSP-2	687:693	TKMSP-2	687:693	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	10	37	from	agent	1312:1316	arg1	medicine					1321:1328	medicine	1321:1328	medicine	1321:1328	It can be applied as a potential immunosuppressant agent in medicine.					
32810537	5	38	theme	containing	807:816	arg1	Man					818:820	containing Man	807:820	containing Man	807:820	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	2	39	theme	extraction	490:499	arg1	follows					400:406	follows	400:406	follows	400:406	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	2	39	theme	extraction	490:499	arg1	26 min					506:511	extraction time 26 min	490:511	extraction time 26 min	490:511	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	7	40	theme	polydisperse	1071:1082	arg1	fractions					1056:1064	the five fractions	1047:1064	the five fractions	1047:1064	UV-vis and FT-IR analysis combined with molecular weight determination further indicated that the five fractions were polydisperse polysaccharides.					
32810537	7	40	theme	polydisperse	1071:1082	arg1	polysaccharides					1084:1098	polydisperse polysaccharides	1071:1098	polydisperse polysaccharides	1071:1098	UV-vis and FT-IR analysis combined with molecular weight determination further indicated that the five fractions were polydisperse polysaccharides.					
32810537	4	41	attach	isolated	731:738	arg2	polysaccharides					661:675	The five polysaccharides	652:675	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5)	652:724	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	41	attach	isolated	731:738	arg2	TKMSP-1					678:684	TKMSP-1	678:684	TKMSP-1	678:684	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	41	attach	isolated	731:738	arg2	TKMSP-4					705:711	TKMSP-4	705:711	TKMSP-4	705:711	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	41	attach	isolated	731:738	arg2	TKMSP-2					687:693	TKMSP-2	687:693	TKMSP-2	687:693	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	41	attach	isolated	731:738	arg1	TKMSP					745:749	TKMSP	745:749	TKMSP	745:749	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	41	attach	isolated	731:738	arg2	TKMSP-3					696:702	TKMSP-3	696:702	TKMSP-3	696:702	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	4	41	attach	isolated	731:738	arg2	TKMSP-5					717:723	TKMSP-5	717:723	TKMSP-5	717:723	The five polysaccharides (TKMSP-1, TKMSP-2, TKMSP-3, TKMSP-4 and TKMSP-5) were isolated from TKMSP by DEAE-52.					
32810537	1	42	dep	kirilowii	225:233	arg1	seeds					241:245	Maxim seeds	235:245	Trichosanthes kirilowii Maxim seeds (TKMSP)	211:253	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	1	42	dep	kirilowii	225:233	arg1	TKMSP					248:252	TKMSP	248:252	TKMSP	248:252	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	8	43	theme	fractions	1188:1196	arg1	characterization					1157:1172	the structural characterization	1142:1172	the structural characterization of these five fractions	1142:1196	A significant difference was achieved in the structural characterization of these five fractions.					
32810537	7	44	theme	weight	1003:1008	arg1	determination					1010:1022	molecular weight determination	993:1022	molecular weight determination	993:1022	UV-vis and FT-IR analysis combined with molecular weight determination further indicated that the five fractions were polydisperse polysaccharides.					
32810537	10	45	theme	immunosuppressant	1294:1310	arg1	agent					1312:1316	a potential immunosuppressant agent	1282:1316	a potential immunosuppressant agent in medicine	1282:1328	It can be applied as a potential immunosuppressant agent in medicine.					
32810537	10	45	theme	immunosuppressant	1294:1310	arg1	It					1261:1262	It	1261:1262	It	1261:1262	It can be applied as a potential immunosuppressant agent in medicine.					
32810537	2	46	theme	extraction	362:371	arg1	conditions					373:382	The optimum extraction conditions	350:382	The optimum extraction conditions	350:382	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	2	46	theme	extraction	362:371	arg1	follows					400:406	follows	400:406	follows	400:406	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	5	47	from	Xyl	855:857	arg1	common					797:802	common	797:802	common	797:802	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	0	48	from	kirilowii	134:142	arg1	seeds					150:154	Maxim seeds	144:154	Maxim seeds	144:154	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	48	from	kirilowii	134:142	arg1	characterization					31:46	characterization	31:46	characterization	31:46	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	48	from	kirilowii	134:142	arg1	activity					69:76	immunomodulatory activity	52:76	immunomodulatory activity	52:76	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	48	from	kirilowii	134:142	arg1	extraction					19:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	5	49	from	common	797:802	arg1	Glu					845:847	Glu	845:847	Glu	845:847	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	5	49	from	common	797:802	arg1	Arab					860:863	Arab	860:863	Arab	860:863	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	5	49	from	common	797:802	arg1	Rha					828:830	Rha	828:830	Rha	828:830	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	5	49	from	common	797:802	arg1	Fuc					869:871	Fuc	869:871	Fuc	869:871	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	5	49	from	common	797:802	arg1	GluA					833:836	GluA	833:836	GluA	833:836	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	5	49	from	common	797:802	arg1	Gal					850:852	Gal	850:852	Gal	850:852	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	5	49	from	common	797:802	arg1	Man					818:820	containing Man	807:820	containing Man	807:820	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	5	49	from	common	797:802	arg1	Xyl					855:857	Xyl	855:857	Xyl	855:857	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	5	49	from	common	797:802	arg1	Rib					823:825	Rib	823:825	Rib	823:825	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	5	49	from	common	797:802	arg1	GalA					839:842	GalA	839:842	GalA	839:842	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	1	50	theme	Microwave-assisted	157:174	arg1	extraction					176:185	Microwave-assisted extraction	157:185	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP)	157:253	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	5	51	from	Man	818:820	arg1	common					797:802	common	797:802	common	797:802	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	3	52	theme	TKMSP	555:559	arg1	value					546:550	the mean value	537:550	the mean value of TKMSP	537:559	Under this conditions, the mean value of TKMSP yield 2.43 ± 0.45% (n = 3), which was consistent closely with the predicted value (2.44%).					
32810537	3	53	theme	predicted	627:635	arg1	value					637:641	the predicted value	623:641	the predicted value (2.44%)	623:649	Under this conditions, the mean value of TKMSP yield 2.43 ± 0.45% (n = 3), which was consistent closely with the predicted value (2.44%).					
32810537	3	53	theme	predicted	627:635	arg1	%					648:648	2.44%	644:648	2.44%	644:648	Under this conditions, the mean value of TKMSP yield 2.43 ± 0.45% (n = 3), which was consistent closely with the predicted value (2.44%).					
32810537	5	54	from	Rib	823:825	arg1	common					797:802	common	797:802	common	797:802	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	5	55	from	Gal	850:852	arg1	common					797:802	common	797:802	common	797:802	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32810537	1	56	theme	Response	275:282	arg1	RSM					305:307	RSM	305:307	RSM	305:307	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	1	56	theme	Response	275:282	arg1	methodology					292:302	Response surface methodology	275:302	Response surface methodology (RSM) base	275:313	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	3	57	theme	mean	541:544	arg1	value					546:550	the mean value	537:550	the mean value of TKMSP	537:559	Under this conditions, the mean value of TKMSP yield 2.43 ± 0.45% (n = 3), which was consistent closely with the predicted value (2.44%).					
32810537	2	58	theme	ratio	422:426	arg1	42 mL/g					428:434	liquid-solid ratio 42 mL/g	409:434	liquid-solid ratio 42 mL/g	409:434	The optimum extraction conditions are detailed as follows: liquid-solid ratio 42 mL/g, extraction temperature 80 °C, microwave power 570 W, extraction time 26 min.					
32810537	1	59	theme	surface	284:290	arg1	RSM					305:307	RSM	305:307	RSM	305:307	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	1	59	theme	surface	284:290	arg1	methodology					292:302	Response surface methodology	275:302	Response surface methodology (RSM) base	275:313	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	0	60	theme	RAW264.7	81:88	arg1	cells					90:94	RAW264.7 cells	81:94	RAW264.7 cells of polysaccharides	81:113	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	0	61	from	characterization	31:46	arg1	cells					90:94	RAW264.7 cells	81:94	RAW264.7 cells of polysaccharides	81:113	Microwave-assisted extraction, characterization and immunomodulatory activity on RAW264.7 cells of polysaccharides from Trichosanthes kirilowii Maxim seeds.					
32810537	1	62	from	kirilowii	225:233	arg1	polysaccharides					190:204	polysaccharides	190:204	polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP)	190:253	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	1	62	from	kirilowii	225:233	arg1	extraction					176:185	Microwave-assisted extraction	157:185	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP)	157:253	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	1	63	theme	polysaccharides	190:204	arg1	extraction					176:185	Microwave-assisted extraction	157:185	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP)	157:253	Microwave-assisted extraction of polysaccharides from Trichosanthes kirilowii Maxim seeds (TKMSP) was optimized using Response surface methodology (RSM) base on Central composite design (CCD).					
32810537	5	64	from	Fuc	869:871	arg1	common					797:802	common	797:802	common	797:802	TKMSP-1, TKMSP-2 and TKMSP-4 were common in containing Man, Rib, Rha, GluA, GalA, Glu, Gal, Xyl, Arab and Fuc.					
32919631	6	0	theme	novel	809:813	arg1	intervention					815:826	an attractive novel intervention	795:826	an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies	795:952	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	6	0	theme	novel	809:813	arg1	probiotics					770:779	composite probiotics	760:779	composite probiotics to wounds	760:789	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	1	1	theme	external	171:178	arg1	environment					180:190	the external environment	167:190	the external environment	167:190	As a new strategy for wound management, probiotics are highly sensitive to the external environment during use.					
32919631	4	2	theme	OBSP-CS-LP	555:564	arg1	hydrogel					545:552	probiotic-bound OBSP-Chitosan composite hydrogel	505:552	probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel)	505:574	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	4	2	theme	OBSP-CS-LP	555:564	arg1	hydrogel					566:573	OBSP-CS-LP hydrogel	555:573	OBSP-CS-LP hydrogel	555:573	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	6	3	theme	attractive	798:807	arg1	intervention					815:826	an attractive novel intervention	795:826	an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies	795:952	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	6	3	theme	attractive	798:807	arg1	probiotics					770:779	composite probiotics	760:779	composite probiotics to wounds	760:789	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	2	4	theme	OBSP	289:292	arg1	hydrogel					295:302	oxidized Bletilla striata polysaccharide (OBSP) hydrogel	247:302	oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics	247:366	In this study, an attempt is made to apply oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics.					
32919631	8	5	from	dressing	1125:1132	arg1	healing					1147:1153	the wound healing	1137:1153	the wound healing	1137:1153	Therefore, OBSP-CS-LP hydrogel could be applied as a promising dressing in the wound healing.					
32919631	5	6	theme	OBSP-CS-LP	712:721	arg1	hydrogel					723:730	the OBSP-CS-LP hydrogel	708:730	the OBSP-CS-LP hydrogel	708:730	Subsequently, the SEM image, swelling and rheological properties of the OBSP-CS-LP hydrogel were investigated.					
32919631	0	7	theme	Novel	0:4	arg1	striata					40:46	Novel probiotic-bound oxidized Bletilla striata	0:46	Novel probiotic-bound oxidized Bletilla striata	0:46	Novel probiotic-bound oxidized Bletilla striata polysaccharide-chitosan composite hydrogel.					
32919631	5	8	theme	hydrogel	723:730	arg1	swelling					669:676	swelling	669:676	swelling	669:676	Subsequently, the SEM image, swelling and rheological properties of the OBSP-CS-LP hydrogel were investigated.					
32919631	5	8	theme	hydrogel	723:730	arg1	properties					694:703	rheological properties	682:703	rheological properties	682:703	Subsequently, the SEM image, swelling and rheological properties of the OBSP-CS-LP hydrogel were investigated.					
32919631	5	8	theme	hydrogel	723:730	arg1	image					662:666	the SEM image	654:666	the SEM image	654:666	Subsequently, the SEM image, swelling and rheological properties of the OBSP-CS-LP hydrogel were investigated.					
32919631	8	9	theme	OBSP-CS-LP	1073:1082	arg1	dressing					1125:1132	a promising dressing	1113:1132	a promising dressing in the wound healing	1113:1153	Therefore, OBSP-CS-LP hydrogel could be applied as a promising dressing in the wound healing.					
32919631	8	9	theme	OBSP-CS-LP	1073:1082	arg1	hydrogel					1084:1091	OBSP-CS-LP hydrogel	1073:1091	OBSP-CS-LP hydrogel	1073:1091	Therefore, OBSP-CS-LP hydrogel could be applied as a promising dressing in the wound healing.					
32919631	2	10	theme	polysaccharide	273:286	arg1	hydrogel					295:302	oxidized Bletilla striata polysaccharide (OBSP) hydrogel	247:302	oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics	247:366	In this study, an attempt is made to apply oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics.					
32919631	3	11	theme	Chinese	411:417	arg1	medicine					419:426	a famous traditional Chinese medicine	390:426	a famous traditional Chinese medicine	390:426	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	3	11	theme	Chinese	411:417	arg1	striata					378:384	Bletilla striata	369:384	Bletilla striata	369:384	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	6	12	theme	hydrogel	892:899	arg1	properties					874:883	the antibacterial properties	856:883	the antibacterial properties of the hydrogel	856:899	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	1	13	theme	new	97:99	arg1	strategy					101:108	a new strategy	95:108	a new strategy for wound management	95:129	As a new strategy for wound management, probiotics are highly sensitive to the external environment during use.					
32919631	6	14	theme	therapies	944:952	arg1	pitfalls					916:923	the pitfalls	912:923	the pitfalls of many antibiotic therapies	912:952	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	0	15	theme	probiotic-bound	6:20	arg1	striata					40:46	Novel probiotic-bound oxidized Bletilla striata	0:46	Novel probiotic-bound oxidized Bletilla striata	0:46	Novel probiotic-bound oxidized Bletilla striata polysaccharide-chitosan composite hydrogel.					
32919631	3	16	theme	famous	392:397	arg1	medicine					419:426	a famous traditional Chinese medicine	390:426	a famous traditional Chinese medicine	390:426	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	3	16	theme	famous	392:397	arg1	striata					378:384	Bletilla striata	369:384	Bletilla striata	369:384	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	3	17	theme	Bletilla	369:376	arg1	medicine					419:426	a famous traditional Chinese medicine	390:426	a famous traditional Chinese medicine	390:426	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	3	17	theme	Bletilla	369:376	arg1	striata					378:384	Bletilla striata	369:384	Bletilla striata	369:384	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	4	18	theme	composite	535:543	arg1	hydrogel					545:552	probiotic-bound OBSP-Chitosan composite hydrogel	505:552	probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel)	505:574	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	4	18	theme	composite	535:543	arg1	hydrogel					566:573	OBSP-CS-LP hydrogel	555:573	OBSP-CS-LP hydrogel	555:573	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	3	19	theme	traditional	399:409	arg1	medicine					419:426	a famous traditional Chinese medicine	390:426	a famous traditional Chinese medicine	390:426	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	3	19	theme	traditional	399:409	arg1	striata					378:384	Bletilla striata	369:384	Bletilla striata	369:384	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	0	20	theme	Bletilla	31:38	arg1	striata					40:46	Novel probiotic-bound oxidized Bletilla striata	0:46	Novel probiotic-bound oxidized Bletilla striata	0:46	Novel probiotic-bound oxidized Bletilla striata polysaccharide-chitosan composite hydrogel.					
32919631	4	21	theme	OBSP-Chitosan	521:533	arg1	hydrogel					545:552	probiotic-bound OBSP-Chitosan composite hydrogel	505:552	probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel)	505:574	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	4	21	theme	OBSP-Chitosan	521:533	arg1	hydrogel					566:573	OBSP-CS-LP hydrogel	555:573	OBSP-CS-LP hydrogel	555:573	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	6	22	theme	composite	760:768	arg1	intervention					815:826	an attractive novel intervention	795:826	an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies	795:952	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	6	22	theme	composite	760:768	arg1	probiotics					770:779	composite probiotics	760:779	composite probiotics to wounds	760:789	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	1	23	theme	wound	114:118	arg1	management					120:129	wound management	114:129	wound management	114:129	As a new strategy for wound management, probiotics are highly sensitive to the external environment during use.					
32919631	7	24	theme	full-thickness	972:985	arg1	model					999:1003	the full-thickness skin defect model	968:1003	the full-thickness skin defect model in vivo	968:1011	Furthermore, the full-thickness skin defect model in vivo indicated an outstanding wound healing efficacy.					
32919631	4	25	theme	environmentally	605:619	arg1	approach					630:637	a simple and environmentally friendly approach	592:637	a simple and environmentally friendly approach	592:637	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	0	26	theme	oxidized	22:29	arg1	striata					40:46	Novel probiotic-bound oxidized Bletilla striata	0:46	Novel probiotic-bound oxidized Bletilla striata	0:46	Novel probiotic-bound oxidized Bletilla striata polysaccharide-chitosan composite hydrogel.					
32919631	5	27	theme	rheological	682:692	arg1	properties					694:703	rheological properties	682:703	rheological properties	682:703	Subsequently, the SEM image, swelling and rheological properties of the OBSP-CS-LP hydrogel were investigated.					
32919631	6	28	theme	antibacterial	860:872	arg1	properties					874:883	the antibacterial properties	856:883	the antibacterial properties of the hydrogel	856:899	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	3	29	used	used	445:448	arg2	medicine					419:426	a famous traditional Chinese medicine	390:426	a famous traditional Chinese medicine	390:426	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	3	29	used	used	445:448	arg2	striata					378:384	Bletilla striata	369:384	Bletilla striata	369:384	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	7	30	theme	outstanding	1026:1036	arg1	healing					1044:1050	an outstanding wound healing	1023:1050	an outstanding wound healing efficacy	1023:1059	Furthermore, the full-thickness skin defect model in vivo indicated an outstanding wound healing efficacy.					
32919631	4	31	theme	friendly	621:628	arg1	approach					630:637	a simple and environmentally friendly approach	592:637	a simple and environmentally friendly approach	592:637	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	5	32	theme	SEM	658:660	arg1	image					662:666	the SEM image	654:666	the SEM image	654:666	Subsequently, the SEM image, swelling and rheological properties of the OBSP-CS-LP hydrogel were investigated.					
32919631	7	33	theme	wound	1038:1042	arg1	healing					1044:1050	an outstanding wound healing	1023:1050	an outstanding wound healing efficacy	1023:1059	Furthermore, the full-thickness skin defect model in vivo indicated an outstanding wound healing efficacy.					
32919631	0	34	theme	composite	72:80	arg1	hydrogel					82:89	composite hydrogel	72:89	composite hydrogel	72:89	Novel probiotic-bound oxidized Bletilla striata polysaccharide-chitosan composite hydrogel.					
32919631	4	35	theme	probiotic-bound	505:519	arg1	hydrogel					545:552	probiotic-bound OBSP-Chitosan composite hydrogel	505:552	probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel)	505:574	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	4	35	theme	probiotic-bound	505:519	arg1	hydrogel					566:573	OBSP-CS-LP hydrogel	555:573	OBSP-CS-LP hydrogel	555:573	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	8	36	theme	promising	1115:1123	arg1	dressing					1125:1132	a promising dressing	1113:1132	a promising dressing in the wound healing	1113:1153	Therefore, OBSP-CS-LP hydrogel could be applied as a promising dressing in the wound healing.					
32919631	8	36	theme	promising	1115:1123	arg1	hydrogel					1084:1091	OBSP-CS-LP hydrogel	1073:1091	OBSP-CS-LP hydrogel	1073:1091	Therefore, OBSP-CS-LP hydrogel could be applied as a promising dressing in the wound healing.					
32919631	6	37	theme	antibiotic	933:942	arg1	therapies					944:952	many antibiotic therapies	928:952	many antibiotic therapies	928:952	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	7	38	theme	healing	1044:1050	arg1	efficacy					1052:1059	an outstanding wound healing efficacy	1023:1059	an outstanding wound healing efficacy	1023:1059	Furthermore, the full-thickness skin defect model in vivo indicated an outstanding wound healing efficacy.					
32919631	2	39	theme	Bletilla	256:263	arg1	hydrogel					295:302	oxidized Bletilla striata polysaccharide (OBSP) hydrogel	247:302	oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics	247:366	In this study, an attempt is made to apply oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics.					
32919631	7	40	theme	skin	987:990	arg1	model					999:1003	the full-thickness skin defect model	968:1003	the full-thickness skin defect model in vivo	968:1011	Furthermore, the full-thickness skin defect model in vivo indicated an outstanding wound healing efficacy.					
32919631	2	41	theme	striata	265:271	arg1	hydrogel					295:302	oxidized Bletilla striata polysaccharide (OBSP) hydrogel	247:302	oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics	247:366	In this study, an attempt is made to apply oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics.					
32919631	2	42	theme	oxidized	247:254	arg1	hydrogel					295:302	oxidized Bletilla striata polysaccharide (OBSP) hydrogel	247:302	oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics	247:366	In this study, an attempt is made to apply oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics.					
32919631	2	43	theme	delivery	309:316	arg1	system					318:323	a delivery system	307:323	a delivery system	307:323	In this study, an attempt is made to apply oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics.					
32919631	3	44	theme	wound	481:485	arg1	healing					487:493	wound healing	481:493	wound healing	481:493	Bletilla striata, as a famous traditional Chinese medicine, has been widely used for over 1500 years to promote wound healing.					
32919631	4	45	theme	simple	594:599	arg1	approach					630:637	a simple and environmentally friendly approach	592:637	a simple and environmentally friendly approach	592:637	Firstly, probiotic-bound OBSP-Chitosan composite hydrogel (OBSP-CS-LP hydrogel) was prepared by a simple and environmentally friendly approach.					
32919631	8	46	theme	wound	1141:1145	arg1	healing					1147:1153	the wound healing	1137:1153	the wound healing	1137:1153	Therefore, OBSP-CS-LP hydrogel could be applied as a promising dressing in the wound healing.					
32919631	6	47	theme	many	928:931	arg1	therapies					944:952	many antibiotic therapies	928:952	many antibiotic therapies	928:952	Notably, composite probiotics to wounds are an attractive novel intervention that significantly improves the antibacterial properties of the hydrogel and avoids the pitfalls of many antibiotic therapies.					
32919631	7	48	theme	defect	992:997	arg1	model					999:1003	the full-thickness skin defect model	968:1003	the full-thickness skin defect model in vivo	968:1011	Furthermore, the full-thickness skin defect model in vivo indicated an outstanding wound healing efficacy.					
32919631	2	49	theme	physical	337:344	arg1	barrier					346:352	a physical barrier	335:352	a physical barrier to probiotics	335:366	In this study, an attempt is made to apply oxidized Bletilla striata polysaccharide (OBSP) hydrogel as a delivery system to put up a physical barrier to probiotics.					
32097734	2	0	theme	linear	448:453	arg1	pectin					455:460	a linear pectin	446:460	a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→	446:508	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	0	theme	linear	448:453	arg1	FP2					439:441	FP2	439:441	FP2	439:441	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	3	1	theme	western	637:643	arg1	analysis					650:657	western blot analysis	637:657	western blot analysis	637:657	We investigated the anti-proliferative activity and its underlying mechanism of FP2 in HeLa cancer cells, using MTT assay and western blot analysis, respectively.					
32097734	7	2	theme	plant	1064:1068	arg1	FP2					1041:1043	FP2	1041:1043	FP2	1041:1043	The results suggest that FP2 may be a promising plant polysaccharide targeting for anticancer therapy through activating the apoptotic pathway.					
32097734	7	2	theme	plant	1064:1068	arg1	polysaccharide					1070:1083	a promising plant polysaccharide	1052:1083	a promising plant polysaccharide targeting for anticancer therapy through activating the apoptotic pathway	1052:1157	The results suggest that FP2 may be a promising plant polysaccharide targeting for anticancer therapy through activating the apoptotic pathway.					
32097734	2	3	theme	composition	345:355	arg1	analysis					357:364	composition analysis	345:364	composition analysis	345:364	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	3	4	theme	MTT	623:625	arg1	assay					627:631	MTT assay	623:631	MTT assay	623:631	We investigated the anti-proliferative activity and its underlying mechanism of FP2 in HeLa cancer cells, using MTT assay and western blot analysis, respectively.					
32097734	1	5	theme	83.3 kDa	160:167	arg1	weight					179:184	83.3 kDa molecular weight	160:184	83.3 kDa molecular weight	160:184	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	6	6	theme	apoptosis	936:944	arg1	induction					946:954	apoptosis induction	936:954	apoptosis induction mediated through caspase-3 activation and cleavage of PARP	936:1013	FP2 has potential of antitumor possibly due to apoptosis induction mediated through caspase-3 activation and cleavage of PARP.					
32097734	0	7	theme	pandurata	107:115	arg1	H					117:117	Ficus pandurata H	101:117	Ficus pandurata H	101:117	Purification, characterization and anti-tumor activity of a pectic-type polysaccharide isolated from Ficus pandurata H.					
32097734	4	8	theme	HeLa	696:699	arg1	cells					708:712	HeLa cancer cells	696:712	HeLa cancer cells	696:712	Treatment with FP2 in HeLa cancer cells showed anti-proliferation effect and up-regulated the expression levels of caspase-3 and cleaved-PARP.					
32097734	0	9	theme	Ficus	101:105	arg1	H					117:117	Ficus pandurata H	101:117	Ficus pandurata H	101:117	Purification, characterization and anti-tumor activity of a pectic-type polysaccharide isolated from Ficus pandurata H.					
32097734	7	10	theme	promising	1054:1062	arg1	FP2					1041:1043	FP2	1041:1043	FP2	1041:1043	The results suggest that FP2 may be a promising plant polysaccharide targeting for anticancer therapy through activating the apoptotic pathway.					
32097734	7	10	theme	promising	1054:1062	arg1	polysaccharide					1070:1083	a promising plant polysaccharide	1052:1083	a promising plant polysaccharide targeting for anticancer therapy through activating the apoptotic pathway	1052:1157	The results suggest that FP2 may be a promising plant polysaccharide targeting for anticancer therapy through activating the apoptotic pathway.					
32097734	2	11	theme	main	469:472	arg1	chain					474:478	a main chain	467:478	a main chain composed of →4)-α-D-GalpA-(1→	467:508	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	1	12	with	polysaccharide	134:147	arg1	weight					179:184	83.3 kDa molecular weight	160:184	83.3 kDa molecular weight	160:184	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	2	13	with	pectin	455:460	arg1	chain					474:478	a main chain	467:478	a main chain composed of →4)-α-D-GalpA-(1→	467:508	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	1	14	theme	molecular	169:177	arg1	weight					179:184	83.3 kDa molecular weight	160:184	83.3 kDa molecular weight	160:184	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	3	15	theme	blot	645:648	arg1	analysis					650:657	western blot analysis	637:657	western blot analysis	637:657	We investigated the anti-proliferative activity and its underlying mechanism of FP2 in HeLa cancer cells, using MTT assay and western blot analysis, respectively.					
32097734	6	16	theme	antitumor	910:918	arg1	potential					897:905	potential	897:905	potential of antitumor	897:918	FP2 has potential of antitumor possibly due to apoptosis induction mediated through caspase-3 activation and cleavage of PARP.					
32097734	2	17	dep	analysis	357:364	arg1	the					332:334	the	332:334	the	332:334	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	17	dep	analysis	357:364	arg1	basis					336:340	basis	336:340	basis	336:340	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	4	18	with	Treatment	674:682	arg1	FP2					689:691	FP2	689:691	FP2	689:691	Treatment with FP2 in HeLa cancer cells showed anti-proliferation effect and up-regulated the expression levels of caspase-3 and cleaved-PARP.					
32097734	4	19	theme	cleaved-PARP	803:814	arg1	levels					779:784	the expression levels	764:784	the expression levels of caspase-3 and cleaved-PARP	764:814	Treatment with FP2 in HeLa cancer cells showed anti-proliferation effect and up-regulated the expression levels of caspase-3 and cleaved-PARP.					
32097734	2	20	theme	spectra	376:382	arg1	pectin					455:460	a linear pectin	446:460	a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→	446:508	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	20	theme	spectra	376:382	arg1	FP2					439:441	FP2	439:441	FP2	439:441	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	7	21	theme	apoptotic	1141:1149	arg1	pathway					1151:1157	the apoptotic pathway	1137:1157	the apoptotic pathway	1137:1157	The results suggest that FP2 may be a promising plant polysaccharide targeting for anticancer therapy through activating the apoptotic pathway.					
32097734	4	22	from	Treatment	674:682	arg1	cells					708:712	HeLa cancer cells	696:712	HeLa cancer cells	696:712	Treatment with FP2 in HeLa cancer cells showed anti-proliferation effect and up-regulated the expression levels of caspase-3 and cleaved-PARP.					
32097734	4	23	theme	caspase-3	789:797	arg1	levels					779:784	the expression levels	764:784	the expression levels of caspase-3 and cleaved-PARP	764:814	Treatment with FP2 in HeLa cancer cells showed anti-proliferation effect and up-regulated the expression levels of caspase-3 and cleaved-PARP.					
32097734	2	24	theme	infrared	367:374	arg1	IR					385:386	IR	385:386	IR	385:386	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	24	theme	infrared	367:374	arg1	spectra					376:382	infrared spectra	367:382	infrared spectra (IR)	367:387	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	1	25	theme	Sevag	257:261	arg1	chromatography					279:292	Sevag, anion-exchange chromatography	257:292	chromatography	279:292	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	2	26	theme	-α-D-GalpA-	495:505	arg1	1→					507:508	→4)-α-D-GalpA-(1→	492:508	→4)-α-D-GalpA-(1→	492:508	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	27	theme	magnetic	401:408	arg1	NMR					421:423	NMR	421:423	NMR	421:423	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	27	theme	magnetic	401:408	arg1	resonance					410:418	nuclear magnetic resonance	393:418	nuclear magnetic resonance (NMR) experiments	393:436	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	28	theme	resonance	410:418	arg1	experiments					426:436	nuclear magnetic resonance (NMR) experiments	393:436	nuclear magnetic resonance (NMR) experiments	393:436	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	29	theme	nuclear	393:399	arg1	NMR					421:423	NMR	421:423	NMR	421:423	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	29	theme	nuclear	393:399	arg1	resonance					410:418	nuclear magnetic resonance	393:418	nuclear magnetic resonance (NMR) experiments	393:436	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	7	30	theme	anticancer	1099:1108	arg1	therapy					1110:1116	anticancer therapy	1099:1116	anticancer therapy	1099:1116	The results suggest that FP2 may be a promising plant polysaccharide targeting for anticancer therapy through activating the apoptotic pathway.					
32097734	1	31	theme	anion-exchange	264:277	arg1	chromatography					279:292	Sevag, anion-exchange chromatography	257:292	chromatography	279:292	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	3	32	theme	HeLa	598:601	arg1	cells					610:614	HeLa cancer cells	598:614	HeLa cancer cells	598:614	We investigated the anti-proliferative activity and its underlying mechanism of FP2 in HeLa cancer cells, using MTT assay and western blot analysis, respectively.					
32097734	3	33	theme	anti-proliferative	531:548	arg1	activity					550:557	the anti-proliferative activity	527:557	the anti-proliferative activity	527:557	We investigated the anti-proliferative activity and its underlying mechanism of FP2 in HeLa cancer cells, using MTT assay and western blot analysis, respectively.					
32097734	1	34	theme	aerial	208:213	arg1	parts					215:219	the aerial parts	204:219	the aerial parts of Ficus pandurata H. (Moraceae)	204:252	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	0	35	theme	anti-tumor	35:44	arg1	activity					46:53	anti-tumor activity	35:53	anti-tumor activity	35:53	Purification, characterization and anti-tumor activity of a pectic-type polysaccharide isolated from Ficus pandurata H.					
32097734	1	36	dep	Ficus	224:228	arg1	H					240:240	pandurata H	230:240	Ficus pandurata H. (Moraceae)	224:252	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	2	37	theme	experiments	426:436	arg1	pectin					455:460	a linear pectin	446:460	a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→	446:508	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	37	theme	experiments	426:436	arg1	FP2					439:441	FP2	439:441	FP2	439:441	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	1	38	theme	gel-filtration	298:311	arg1	chromatography					313:326	gel-filtration chromatography	298:326	gel-filtration chromatography	298:326	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	3	39	theme	FP2	591:593	arg1	activity					550:557	the anti-proliferative activity	527:557	the anti-proliferative activity	527:557	We investigated the anti-proliferative activity and its underlying mechanism of FP2 in HeLa cancer cells, using MTT assay and western blot analysis, respectively.					
32097734	3	39	theme	FP2	591:593	arg1	mechanism					578:586	its underlying mechanism	563:586	its underlying mechanism of FP2	563:593	We investigated the anti-proliferative activity and its underlying mechanism of FP2 in HeLa cancer cells, using MTT assay and western blot analysis, respectively.					
32097734	4	40	theme	expression	768:777	arg1	levels					779:784	the expression levels	764:784	the expression levels of caspase-3 and cleaved-PARP	764:814	Treatment with FP2 in HeLa cancer cells showed anti-proliferation effect and up-regulated the expression levels of caspase-3 and cleaved-PARP.					
32097734	1	41	theme	homogeneous	122:132	arg1	polysaccharide					134:147	A homogeneous polysaccharide	120:147	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight	120:184	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	1	41	theme	homogeneous	122:132	arg1	FP2					150:152	FP2	150:152	FP2	150:152	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	4	42	theme	anti-proliferation	721:738	arg1	effect					740:745	anti-proliferation effect	721:745	anti-proliferation effect	721:745	Treatment with FP2 in HeLa cancer cells showed anti-proliferation effect and up-regulated the expression levels of caspase-3 and cleaved-PARP.					
32097734	3	43	theme	underlying	567:576	arg1	mechanism					578:586	its underlying mechanism	563:586	its underlying mechanism of FP2	563:593	We investigated the anti-proliferative activity and its underlying mechanism of FP2 in HeLa cancer cells, using MTT assay and western blot analysis, respectively.					
32097734	6	44	theme	caspase-3	973:981	arg1	activation					983:992	caspase-3 activation	973:992	caspase-3 activation	973:992	FP2 has potential of antitumor possibly due to apoptosis induction mediated through caspase-3 activation and cleavage of PARP.					
32097734	4	45	theme	cancer	701:706	arg1	cells					708:712	HeLa cancer cells	696:712	HeLa cancer cells	696:712	Treatment with FP2 in HeLa cancer cells showed anti-proliferation effect and up-regulated the expression levels of caspase-3 and cleaved-PARP.					
32097734	3	46	theme	cancer	603:608	arg1	cells					610:614	HeLa cancer cells	598:614	HeLa cancer cells	598:614	We investigated the anti-proliferative activity and its underlying mechanism of FP2 in HeLa cancer cells, using MTT assay and western blot analysis, respectively.					
32097734	1	47	theme	Ficus	224:228	arg1	parts					215:219	the aerial parts	204:219	the aerial parts of Ficus pandurata H. (Moraceae)	204:252	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	0	48	theme	polysaccharide	72:85	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and anti-tumor activity of a pectic-type polysaccharide isolated from Ficus pandurata H.					
32097734	0	48	theme	polysaccharide	72:85	arg1	activity					46:53	anti-tumor activity	35:53	anti-tumor activity	35:53	Purification, characterization and anti-tumor activity of a pectic-type polysaccharide isolated from Ficus pandurata H.					
32097734	0	48	theme	polysaccharide	72:85	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and anti-tumor activity of a pectic-type polysaccharide isolated from Ficus pandurata H.					
32097734	6	49	theme	PARP	1010:1013	arg1	cleavage					998:1005	cleavage	998:1005	cleavage of PARP	998:1013	FP2 has potential of antitumor possibly due to apoptosis induction mediated through caspase-3 activation and cleavage of PARP.					
32097734	6	49	theme	PARP	1010:1013	arg1	activation					983:992	caspase-3 activation	973:992	caspase-3 activation	973:992	FP2 has potential of antitumor possibly due to apoptosis induction mediated through caspase-3 activation and cleavage of PARP.					
32097734	5	50	theme	IC50	817:820	arg1	values					822:827	IC50 values	817:827	IC50 values	817:827	IC50 values were 31.50 and 22.62 μg/ml for 24 h and 48 h, respectively.					
32097734	1	51	theme	pandurata	230:238	arg1	H					240:240	pandurata H	230:240	Ficus pandurata H. (Moraceae)	224:252	A homogeneous polysaccharide (FP2) with 83.3 kDa molecular weight was obtained from the aerial parts of Ficus pandurata H. (Moraceae) by Sevag, anion-exchange chromatography and gel-filtration chromatography.					
32097734	0	52	theme	pectic-type	60:70	arg1	polysaccharide					72:85	a pectic-type polysaccharide	58:85	a pectic-type polysaccharide	58:85	Purification, characterization and anti-tumor activity of a pectic-type polysaccharide isolated from Ficus pandurata H.					
32097734	6	53	contain	has	893:895	arg1	FP2					889:891	FP2	889:891	FP2	889:891	FP2 has potential of antitumor possibly due to apoptosis induction mediated through caspase-3 activation and cleavage of PARP.					
32097734	6	53	contain	has	893:895	arg2	potential					897:905	potential	897:905	potential of antitumor	897:918	FP2 has potential of antitumor possibly due to apoptosis induction mediated through caspase-3 activation and cleavage of PARP.					
32097734	2	54	theme	analysis	357:364	arg1	pectin					455:460	a linear pectin	446:460	a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→	446:508	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32097734	2	54	theme	analysis	357:364	arg1	FP2					439:441	FP2	439:441	FP2	439:441	On the basis of composition analysis, infrared spectra (IR) and nuclear magnetic resonance (NMR) experiments, FP2 is a linear pectin with a main chain composed of →4)-α-D-GalpA-(1→.					
32299557	0	0	from	heterogeneity	14:26	arg1	sapwood					59:65	sapwood	59:65	sapwood	59:65	Unmasking the heterogeneity of carbohydrates in heartwood, sapwood, and bark of Eucalyptus.					
32299557	0	0	from	heterogeneity	14:26	arg1	bark					72:75	bark	72:75	bark	72:75	Unmasking the heterogeneity of carbohydrates in heartwood, sapwood, and bark of Eucalyptus.					
32299557	0	0	from	heterogeneity	14:26	arg1	heartwood					48:56	heartwood	48:56	heartwood	48:56	Unmasking the heterogeneity of carbohydrates in heartwood, sapwood, and bark of Eucalyptus.					
32299557	3	1	theme	weights	544:550	arg1	XRD					577:579	XRD	577:579	XRD	577:579	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	3	1	theme	weights	544:550	arg1	techniques					589:598	NMR techniques	585:598	NMR techniques	585:598	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	3	1	theme	weights	544:550	arg1	spectroscopy					559:570	FT-IR spectroscopy	553:570	FT-IR spectroscopy	553:570	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	3	1	theme	weights	544:550	arg1	determination					517:529	determination	517:529	determination of molecular weights	517:550	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	3	1	theme	weights	544:550	arg1	analyses					507:514	compositional carbohydrate analyses	480:514	compositional carbohydrate analyses	480:514	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	5	2	contain	had	787:789	arg2	polydispersity					887:900	narrower polydispersity	878:900	narrower polydispersity	878:900	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	5	2	contain	had	787:789	arg1	hemicelluloses					759:772	the hemicelluloses	755:772	Meanwhile the hemicelluloses in heartwood	745:785	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	5	2	contain	had	787:789	arg2	weights					866:872	slightly lower molecular weights	841:872	slightly lower molecular weights	841:872	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	5	2	contain	had	787:789	arg2	degree					820:825	lower degree	814:825	lower degree of branching	814:838	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	5	2	contain	had	787:789	arg2	content					805:811	higher xylose content	791:811	higher xylose content	791:811	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	1	3	from	hemicelluloses	125:138	arg1	sapwood					154:160	sapwood	154:160	sapwood	154:160	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	1	3	from	hemicelluloses	125:138	arg1	bark					167:170	bark	167:170	bark	167:170	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	1	3	from	hemicelluloses	125:138	arg1	heartwood					143:151	heartwood	143:151	heartwood	143:151	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	2	4	theme	Raman	334:338	arg1	microscopy					340:349	confocal Raman microscopy	325:349	confocal Raman microscopy	325:349	The ultrastructural topochemistry of carbohydrates in cell walls was examined in situ by confocal Raman microscopy.					
32299557	6	5	theme	side	1021:1024	arg1	chains					1026:1031	glucuronic acid side chains	1005:1031	glucuronic acid side chains	1005:1031	The eucalyptus hemicelluloses mainly consisted of (1→4)-β-D-xylan backbone with glucuronic acid side chains.					
32299557	0	6	theme	Eucalyptus	80:89	arg1	sapwood					59:65	sapwood	59:65	sapwood	59:65	Unmasking the heterogeneity of carbohydrates in heartwood, sapwood, and bark of Eucalyptus.					
32299557	0	6	theme	Eucalyptus	80:89	arg1	bark					72:75	bark	72:75	bark	72:75	Unmasking the heterogeneity of carbohydrates in heartwood, sapwood, and bark of Eucalyptus.					
32299557	0	6	theme	Eucalyptus	80:89	arg1	heartwood					48:56	heartwood	48:56	heartwood	48:56	Unmasking the heterogeneity of carbohydrates in heartwood, sapwood, and bark of Eucalyptus.					
32299557	6	7	theme	acid	1016:1019	arg1	chains					1026:1031	glucuronic acid side chains	1005:1031	glucuronic acid side chains	1005:1031	The eucalyptus hemicelluloses mainly consisted of (1→4)-β-D-xylan backbone with glucuronic acid side chains.					
32299557	3	8	theme	Cellulose	352:360	arg1	samples					400:406	Cellulose and alkali-extractable hemicelluloses samples	352:406	Cellulose and alkali-extractable hemicelluloses samples	352:406	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	7	9	with	degree	1101:1106	arg1	galactose					1138:1146	terminal galactose	1129:1146	terminal galactose	1129:1146	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	1	10	from	cellulose	111:119	arg1	sapwood					154:160	sapwood	154:160	sapwood	154:160	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	1	10	from	cellulose	111:119	arg1	bark					167:170	bark	167:170	bark	167:170	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	1	10	from	cellulose	111:119	arg1	heartwood					143:151	heartwood	143:151	heartwood	143:151	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	2	11	theme	carbohydrates	273:285	arg1	topochemistry					256:268	The ultrastructural topochemistry	236:268	The ultrastructural topochemistry of carbohydrates in cell walls	236:299	The ultrastructural topochemistry of carbohydrates in cell walls was examined in situ by confocal Raman microscopy.					
32299557	6	12	theme	glucuronic	1005:1014	arg1	chains					1026:1031	glucuronic acid side chains	1005:1031	glucuronic acid side chains	1005:1031	The eucalyptus hemicelluloses mainly consisted of (1→4)-β-D-xylan backbone with glucuronic acid side chains.					
32299557	4	13	theme	highest	673:679	arg1	weight					691:696	molecular weight	681:696	the highest molecular weight as well as the lowest degree of crystallinity	669:742	It was found that among all of the samples, heartwood cellulose had the highest molecular weight as well as the lowest degree of crystallinity.					
32299557	6	14	dep	hemicelluloses	940:953	arg1	consisted					962:970	consisted	962:970	hemicelluloses mainly consisted of (1→4)-β-D-xylan backbone with glucuronic acid side chains	940:1031	The eucalyptus hemicelluloses mainly consisted of (1→4)-β-D-xylan backbone with glucuronic acid side chains.					
32299557	7	15	theme	terminal	1129:1136	arg1	galactose					1138:1146	terminal galactose	1129:1146	terminal galactose	1129:1146	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	6	16	with	backbone	991:998	arg1	chains					1026:1031	glucuronic acid side chains	1005:1031	glucuronic acid side chains	1005:1031	The eucalyptus hemicelluloses mainly consisted of (1→4)-β-D-xylan backbone with glucuronic acid side chains.					
32299557	7	17	attach	isolated	1066:1073	arg2	hemicelluloses					1051:1064	the hemicelluloses	1047:1064	the hemicelluloses isolated from sapwood	1047:1086	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	7	17	attach	isolated	1066:1073	arg1	sapwood					1080:1086	sapwood	1080:1086	sapwood	1080:1086	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	5	18	theme	narrower	878:885	arg1	polydispersity					887:900	narrower polydispersity	878:900	narrower polydispersity	878:900	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	3	19	theme	hemicelluloses	385:398	arg1	samples					400:406	Cellulose and alkali-extractable hemicelluloses samples	352:406	Cellulose and alkali-extractable hemicelluloses samples	352:406	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	5	20	theme	higher	791:796	arg1	content					805:811	higher xylose content	791:811	higher xylose content	791:811	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	7	21	theme	substitution	1111:1122	arg1	degree					1101:1106	a higher degree	1092:1106	a higher degree of substitution with terminal galactose	1092:1146	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	4	22	theme	molecular	681:689	arg1	weight					691:696	molecular weight	681:696	the highest molecular weight as well as the lowest degree of crystallinity	669:742	It was found that among all of the samples, heartwood cellulose had the highest molecular weight as well as the lowest degree of crystallinity.					
32299557	5	23	theme	branching	830:838	arg1	degree					820:825	lower degree	814:825	lower degree of branching	814:838	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	7	24	theme	higher	1094:1099	arg1	degree					1101:1106	a higher degree	1092:1106	a higher degree of substitution with terminal galactose	1092:1146	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	3	25	theme	NMR	585:587	arg1	techniques					589:598	NMR techniques	585:598	NMR techniques	585:598	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	7	26	contain	had	1088:1090	arg2	degree					1101:1106	a higher degree	1092:1106	a higher degree of substitution with terminal galactose	1092:1146	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	7	26	contain	had	1088:1090	arg1	hemicelluloses					1051:1064	the hemicelluloses	1047:1064	the hemicelluloses isolated from sapwood	1047:1086	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	3	27	theme	carbohydrate	494:505	arg1	analyses					507:514	compositional carbohydrate analyses	480:514	compositional carbohydrate analyses	480:514	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	3	28	theme	alkali-extractable	366:383	arg1	hemicelluloses					385:398	alkali-extractable hemicelluloses	366:398	alkali-extractable hemicelluloses	366:398	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	6	29	theme	-β-D-xylan	980:989	arg1	backbone					991:998	(1→4)-β-D-xylan backbone	975:998	(1→4)-β-D-xylan backbone with glucuronic acid side chains	975:1031	The eucalyptus hemicelluloses mainly consisted of (1→4)-β-D-xylan backbone with glucuronic acid side chains.					
32299557	2	30	theme	cell	290:293	arg1	walls					295:299	cell walls	290:299	cell walls	290:299	The ultrastructural topochemistry of carbohydrates in cell walls was examined in situ by confocal Raman microscopy.					
32299557	5	31	theme	lower	850:854	arg1	weights					866:872	slightly lower molecular weights	841:872	slightly lower molecular weights	841:872	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	5	32	theme	xylose	798:803	arg1	content					805:811	higher xylose content	791:811	higher xylose content	791:811	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	4	33	theme	lowest	713:718	arg1	degree					720:725	the lowest degree	709:725	the highest molecular weight as well as the lowest degree of crystallinity	669:742	It was found that among all of the samples, heartwood cellulose had the highest molecular weight as well as the lowest degree of crystallinity.					
32299557	4	34	contain	had	665:667	arg1	cellulose					655:663	heartwood cellulose	645:663	heartwood cellulose	645:663	It was found that among all of the samples, heartwood cellulose had the highest molecular weight as well as the lowest degree of crystallinity.					
32299557	4	34	contain	had	665:667	arg2	weight					691:696	molecular weight	681:696	the highest molecular weight as well as the lowest degree of crystallinity	669:742	It was found that among all of the samples, heartwood cellulose had the highest molecular weight as well as the lowest degree of crystallinity.					
32299557	4	34	contain	had	665:667	arg2	degree					720:725	the lowest degree	709:725	the highest molecular weight as well as the lowest degree of crystallinity	669:742	It was found that among all of the samples, heartwood cellulose had the highest molecular weight as well as the lowest degree of crystallinity.					
32299557	5	35	theme	molecular	856:864	arg1	weights					866:872	slightly lower molecular weights	841:872	slightly lower molecular weights	841:872	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	0	36	theme	carbohydrates	31:43	arg1	heterogeneity					14:26	the heterogeneity	10:26	the heterogeneity of carbohydrates in heartwood, sapwood, and bark of Eucalyptus	10:89	Unmasking the heterogeneity of carbohydrates in heartwood, sapwood, and bark of Eucalyptus.					
32299557	6	37	theme	1→4	976:978	arg1	backbone					991:998	(1→4)-β-D-xylan backbone	975:998	(1→4)-β-D-xylan backbone with glucuronic acid side chains	975:1031	The eucalyptus hemicelluloses mainly consisted of (1→4)-β-D-xylan backbone with glucuronic acid side chains.					
32299557	3	38	theme	different	427:435	arg1	tissues					437:443	different tissues	427:443	different tissues	427:443	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	3	39	attach	isolated	413:420	arg1	tissues					437:443	different tissues	427:443	different tissues	427:443	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	3	39	attach	isolated	413:420	arg2	samples					400:406	Cellulose and alkali-extractable hemicelluloses samples	352:406	Cellulose and alkali-extractable hemicelluloses samples	352:406	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	3	40	gly	carbohydrate	494:505	arg1	weights					544:550	molecular weights	534:550	molecular weights	534:550	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	1	41	theme	E.	175:176	arg1	grandis					193:199	E. urophylla × E. grandis	175:199	E. urophylla × E. grandis	175:199	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	5	42	from	hemicelluloses	759:772	arg1	heartwood					777:785	heartwood	777:785	heartwood	777:785	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	0	43	gly	heterogeneity	14:26	arg1	carbohydrates					31:43	carbohydrates	31:43	carbohydrates	31:43	Unmasking the heterogeneity of carbohydrates in heartwood, sapwood, and bark of Eucalyptus.					
32299557	2	44	theme	confocal	325:332	arg1	microscopy					340:349	confocal Raman microscopy	325:349	confocal Raman microscopy	325:349	The ultrastructural topochemistry of carbohydrates in cell walls was examined in situ by confocal Raman microscopy.					
32299557	5	45	theme	lower	814:818	arg1	degree					820:825	lower degree	814:825	lower degree of branching	814:838	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32299557	3	46	theme	molecular	534:542	arg1	weights					544:550	molecular weights	534:550	molecular weights	534:550	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	2	47	theme	ultrastructural	240:254	arg1	topochemistry					256:268	The ultrastructural topochemistry	236:268	The ultrastructural topochemistry of carbohydrates in cell walls	236:299	The ultrastructural topochemistry of carbohydrates in cell walls was examined in situ by confocal Raman microscopy.					
32299557	4	48	theme	crystallinity	730:742	arg1	degree					720:725	the lowest degree	709:725	the highest molecular weight as well as the lowest degree of crystallinity	669:742	It was found that among all of the samples, heartwood cellulose had the highest molecular weight as well as the lowest degree of crystallinity.					
32299557	4	48	theme	crystallinity	730:742	arg1	weight					691:696	molecular weight	681:696	the highest molecular weight as well as the lowest degree of crystallinity	669:742	It was found that among all of the samples, heartwood cellulose had the highest molecular weight as well as the lowest degree of crystallinity.					
32299557	2	49	from	topochemistry	256:268	arg1	walls					295:299	cell walls	290:299	cell walls	290:299	The ultrastructural topochemistry of carbohydrates in cell walls was examined in situ by confocal Raman microscopy.					
32299557	1	50	theme	urophylla × E.	178:191	arg1	grandis					193:199	E. urophylla × E. grandis	175:199	E. urophylla × E. grandis	175:199	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	1	51	theme	grandis	193:199	arg1	sapwood					154:160	sapwood	154:160	sapwood	154:160	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	1	51	theme	grandis	193:199	arg1	bark					167:170	bark	167:170	bark	167:170	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	1	51	theme	grandis	193:199	arg1	heartwood					143:151	heartwood	143:151	heartwood	143:151	In this study, the cellulose and hemicelluloses in heartwood, sapwood, and bark of E. urophylla × E. grandis were comprehensively investigated.					
32299557	3	52	theme	compositional	480:492	arg1	analyses					507:514	compositional carbohydrate analyses	480:514	compositional carbohydrate analyses	480:514	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	4	53	theme	heartwood	645:653	arg1	cellulose					655:663	heartwood cellulose	645:663	heartwood cellulose	645:663	It was found that among all of the samples, heartwood cellulose had the highest molecular weight as well as the lowest degree of crystallinity.					
32299557	3	54	theme	FT-IR	553:557	arg1	spectroscopy					559:570	FT-IR spectroscopy	553:570	FT-IR spectroscopy	553:570	Cellulose and alkali-extractable hemicelluloses samples were isolated from different tissues and comparatively characterized by compositional carbohydrate analyses, determination of molecular weights, FT-IR spectroscopy, and XRD and NMR techniques.					
32299557	7	55	attach	isolated	1159:1166	arg1	bark					1187:1190	bark	1187:1190	bark	1187:1190	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	7	55	attach	isolated	1159:1166	arg2	those					1153:1157	those	1153:1157	those	1153:1157	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	7	55	attach	isolated	1159:1166	arg1	heartwood					1173:1181	heartwood	1173:1181	heartwood	1173:1181	Furthermore, the hemicelluloses isolated from sapwood had a higher degree of substitution with terminal galactose than those isolated from heartwood and bark.					
32299557	5	56	theme	Meanwhile	745:753	arg1	hemicelluloses					759:772	the hemicelluloses	755:772	Meanwhile the hemicelluloses in heartwood	745:785	Meanwhile the hemicelluloses in heartwood had higher xylose content, lower degree of branching, slightly lower molecular weights but narrower polydispersity than those in sapwood.					
32163776	0	0	theme	Sodium	96:101	arg1	Nanocomposites					112:125	Organic Montmorillonite Sodium Alginate Nanocomposites	72:125	Organic Montmorillonite Sodium Alginate Nanocomposites	72:125	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	1	1	theme	promising	176:184	arg1	technique					186:194	a promising technique	174:194	a promising technique to remove inorganic and organic contaminants in an economically and environmentally friendly superior manner	174:303	The adsorption method is generally considered a promising technique to remove inorganic and organic contaminants in an economically and environmentally friendly superior manner.					
32163776	1	2	theme	superior	289:296	arg1	manner					298:303	an economically and environmentally friendly superior manner	244:303	an economically and environmentally friendly superior manner	244:303	The adsorption method is generally considered a promising technique to remove inorganic and organic contaminants in an economically and environmentally friendly superior manner.					
32163776	0	3	theme	Montmorillonite	80:94	arg1	Nanocomposites					112:125	Organic Montmorillonite Sodium Alginate Nanocomposites	72:125	Organic Montmorillonite Sodium Alginate Nanocomposites	72:125	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	3	4	from	solution	835:842	arg1	experiments					810:820	batch adsorption experiments	793:820	batch adsorption experiments from aqueous solution	793:842	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	0	5	from	Adsorption	0:9	arg1	solution					60:67	aqueous solution	52:67	aqueous solution	52:67	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	2	6	theme	organic	321:327	arg1	composites					361:370	organic montmorillonite sodium alginate composites	321:370	organic montmorillonite sodium alginate composites	321:370	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	3	7	theme	composites	615:624	arg1	properties					597:606	The morphological properties	579:606	The morphological properties of the composites	579:624	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	0	8	theme	Alginate	103:110	arg1	Nanocomposites					112:125	Organic Montmorillonite Sodium Alginate Nanocomposites	72:125	Organic Montmorillonite Sodium Alginate Nanocomposites	72:125	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	0	9	from	solution	60:67	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.	0:126	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	0	9	from	solution	60:67	arg1	Hydrocarbons					34:45	Polycyclic Aromatic Hydrocarbons	14:45	Polycyclic Aromatic Hydrocarbons from aqueous solution	14:67	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	7	10	theme	multilayer	1192:1201	arg1	adsorption					1203:1212	multilayer adsorption	1192:1212	multilayer adsorption on the composite's energetically heterogeneous surface	1192:1267	According to these, the adsorption process occurred via multilayer adsorption on the composite's energetically heterogeneous surface.					
32163776	5	11	theme	methyl	974:979	arg1	alcohol					981:987	methyl alcohol	974:987	methyl alcohol	974:987	The composites could be stably separated and regenerated with methyl alcohol.					
32163776	2	12	theme	crosslinking	559:570	arg1	CaCl2					546:550	CaCl2	546:550	CaCl2	546:550	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	2	12	theme	crosslinking	559:570	arg1	agent					572:576	the crosslinking agent	555:576	the crosslinking agent	555:576	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	8	13	theme	adsorption	1344:1353	arg1	mechanism					1355:1363	the adsorption mechanism	1340:1363	the adsorption mechanism	1340:1363	Moreover, pore diffusion and hydrophobicity played a dominant role in the adsorption mechanism.					
32163776	2	14	theme	cetyltrimethylammonium	438:459	arg1	CTAB					470:473	CTAB	470:473	CTAB	470:473	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	2	14	theme	cetyltrimethylammonium	438:459	arg1	bromide					461:467	cetyltrimethylammonium bromide	438:467	cetyltrimethylammonium bromide	438:467	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	3	15	theme	morphological	583:595	arg1	properties					597:606	The morphological properties	579:606	The morphological properties of the composites	579:624	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	7	16	from	adsorption	1203:1212	arg1	surface					1261:1267	the composite's energetically heterogeneous surface	1217:1267	the composite's energetically heterogeneous surface	1217:1267	According to these, the adsorption process occurred via multilayer adsorption on the composite's energetically heterogeneous surface.					
32163776	8	17	theme	pore	1280:1283	arg1	diffusion					1285:1293	pore diffusion	1280:1293	pore diffusion	1280:1293	Moreover, pore diffusion and hydrophobicity played a dominant role in the adsorption mechanism.					
32163776	1	18	theme	inorganic	206:214	arg1	contaminants					228:239	inorganic and organic contaminants	206:239	inorganic and organic contaminants	206:239	The adsorption method is generally considered a promising technique to remove inorganic and organic contaminants in an economically and environmentally friendly superior manner.					
32163776	2	19	theme	added	489:493	arg1	amounts					495:501	different added amounts	479:501	different added amounts	479:501	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	7	20	theme	adsorption	1160:1169	arg1	process					1171:1177	the adsorption process	1156:1177	the adsorption process	1156:1177	According to these, the adsorption process occurred via multilayer adsorption on the composite's energetically heterogeneous surface.					
32163776	2	21	theme	alginate	352:359	arg1	composites					361:370	organic montmorillonite sodium alginate composites	321:370	organic montmorillonite sodium alginate composites	321:370	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	9	22	contain	has	1419:1421	arg1	adsorbent					1404:1412	adsorbent	1404:1412	adsorbent	1404:1412	Overall, our study offers a developed adsorbent that has the advantage of being recyclable, low cost, biodegradable and biocompatible for effectively removing PAHs from aqueous solution.					
32163776	9	22	contain	has	1419:1421	arg2	advantage					1427:1435	the advantage	1423:1435	the advantage of being recyclable, low cost, biodegradable and biocompatible for effectively removing PAHs from aqueous solution	1423:1550	Overall, our study offers a developed adsorbent that has the advantage of being recyclable, low cost, biodegradable and biocompatible for effectively removing PAHs from aqueous solution.					
32163776	2	23	theme	cationic	417:424	arg1	surfactant					426:435	cationic surfactant	417:435	cationic surfactant (cetyltrimethylammonium bromide, CTAB)	417:474	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	2	24	theme	different	479:487	arg1	amounts					495:501	different added amounts	479:501	different added amounts	479:501	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	2	25	from	montmorillonite	397:411	arg1	amounts					495:501	different added amounts	479:501	different added amounts	479:501	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	2	26	theme	sodium	345:350	arg1	composites					361:370	organic montmorillonite sodium alginate composites	321:370	organic montmorillonite sodium alginate composites	321:370	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	1	27	theme	organic	220:226	arg1	contaminants					228:239	inorganic and organic contaminants	206:239	inorganic and organic contaminants	206:239	The adsorption method is generally considered a promising technique to remove inorganic and organic contaminants in an economically and environmentally friendly superior manner.					
32163776	0	28	theme	Polycyclic	14:23	arg1	Hydrocarbons					34:45	Polycyclic Aromatic Hydrocarbons	14:45	Polycyclic Aromatic Hydrocarbons from aqueous solution	14:67	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	3	29	theme	batch	793:797	arg1	experiments					810:820	batch adsorption experiments	793:820	batch adsorption experiments from aqueous solution	793:842	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	30	theme	aromatic	718:725	arg1	fluorene					762:769	fluorene	762:769	fluorene	762:769	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	30	theme	aromatic	718:725	arg1	PAHs					741:744	PAHs	741:744	PAHs	741:744	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	30	theme	aromatic	718:725	arg1	acenaphthene					748:759	acenaphthene	748:759	acenaphthene	748:759	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	30	theme	aromatic	718:725	arg1	hydrocarbons					727:738	polycyclic aromatic hydrocarbons	707:738	polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene)	707:788	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	30	theme	aromatic	718:725	arg1	phenanthrene					776:787	phenanthrene	776:787	phenanthrene	776:787	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	2	31	theme	montmorillonite	329:343	arg1	composites					361:370	organic montmorillonite sodium alginate composites	321:370	organic montmorillonite sodium alginate composites	321:370	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	2	32	theme	sodium	524:529	arg1	alginate					531:538	sodium alginate	524:538	sodium alginate using CaCl2 as the crosslinking agent	524:576	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	3	33	theme	adsorption	799:808	arg1	experiments					810:820	batch adsorption experiments	793:820	batch adsorption experiments from aqueous solution	793:842	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	34	theme	hydrocarbons	727:738	arg1	pollutants					693:702	three typical target pollutants	672:702	three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene)	672:788	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	0	35	theme	Hydrocarbons	34:45	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.	0:126	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	6	36	theme	isotherm	1030:1037	arg1	data					1039:1042	the adsorption kinetic and isotherm data	1003:1042	data	1039:1042	Furthermore, the adsorption kinetic and isotherm data were well described by the Elovich kinetic and the Freundlich isotherm model, respectively.					
32163776	0	37	theme	Aromatic	25:32	arg1	Hydrocarbons					34:45	Polycyclic Aromatic Hydrocarbons	14:45	Polycyclic Aromatic Hydrocarbons from aqueous solution	14:67	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	2	38	from	surfactant	426:435	arg1	amounts					495:501	different added amounts	479:501	different added amounts	479:501	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	8	39	theme	dominant	1323:1330	arg1	role					1332:1335	a dominant role	1321:1335	a dominant role	1321:1335	Moreover, pore diffusion and hydrophobicity played a dominant role in the adsorption mechanism.					
32163776	3	40	theme	polycyclic	707:716	arg1	fluorene					762:769	fluorene	762:769	fluorene	762:769	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	40	theme	polycyclic	707:716	arg1	PAHs					741:744	PAHs	741:744	PAHs	741:744	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	40	theme	polycyclic	707:716	arg1	acenaphthene					748:759	acenaphthene	748:759	acenaphthene	748:759	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	40	theme	polycyclic	707:716	arg1	hydrocarbons					727:738	polycyclic aromatic hydrocarbons	707:738	polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene)	707:788	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	40	theme	polycyclic	707:716	arg1	phenanthrene					776:787	phenanthrene	776:787	phenanthrene	776:787	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	1	41	theme	adsorption	132:141	arg1	method					143:148	The adsorption method	128:148	The adsorption method	128:148	The adsorption method is generally considered a promising technique to remove inorganic and organic contaminants in an economically and environmentally friendly superior manner.					
32163776	0	42	theme	aqueous	52:58	arg1	solution					60:67	aqueous solution	52:67	aqueous solution	52:67	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	3	43	theme	aqueous	827:833	arg1	solution					835:842	aqueous solution	827:842	aqueous solution	827:842	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	4	44	theme	PAHs	897:900	arg1	removals					902:909	PAHs removals	897:909	PAHs removals	897:909	The composites provide an efficient alternative for PAHs removals.					
32163776	7	45	theme	heterogeneous	1247:1259	arg1	surface					1261:1267	the composite's energetically heterogeneous surface	1217:1267	the composite's energetically heterogeneous surface	1217:1267	According to these, the adsorption process occurred via multilayer adsorption on the composite's energetically heterogeneous surface.					
32163776	3	46	theme	target	686:691	arg1	pollutants					693:702	three typical target pollutants	672:702	three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene)	672:788	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	2	47	dep	surfactant	426:435	arg1	CTAB					470:473	CTAB	470:473	CTAB	470:473	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	2	47	dep	surfactant	426:435	arg1	bromide					461:467	cetyltrimethylammonium bromide	438:467	cetyltrimethylammonium bromide	438:467	In this study, organic montmorillonite sodium alginate composites were prepared, in which, montmorillonite and cationic surfactant (cetyltrimethylammonium bromide, CTAB) in different added amounts were coagulated with sodium alginate using CaCl2 as the crosslinking agent.					
32163776	6	48	theme	isotherm	1106:1113	arg1	model					1115:1119	the Freundlich isotherm model	1091:1119	the Freundlich isotherm model	1091:1119	Furthermore, the adsorption kinetic and isotherm data were well described by the Elovich kinetic and the Freundlich isotherm model, respectively.					
32163776	6	49	theme	Freundlich	1095:1104	arg1	model					1115:1119	the Freundlich isotherm model	1091:1119	the Freundlich isotherm model	1091:1119	Furthermore, the adsorption kinetic and isotherm data were well described by the Elovich kinetic and the Freundlich isotherm model, respectively.					
32163776	0	50	theme	Organic	72:78	arg1	Nanocomposites					112:125	Organic Montmorillonite Sodium Alginate Nanocomposites	72:125	Organic Montmorillonite Sodium Alginate Nanocomposites	72:125	Adsorption of Polycyclic Aromatic Hydrocarbons from aqueous solution by Organic Montmorillonite Sodium Alginate Nanocomposites.					
32163776	4	51	theme	efficient	871:879	arg1	alternative					881:891	an efficient alternative	868:891	an efficient alternative for PAHs removals	868:909	The composites provide an efficient alternative for PAHs removals.					
32163776	9	52	theme	aqueous	1535:1541	arg1	solution					1543:1550	aqueous solution	1535:1550	aqueous solution	1535:1550	Overall, our study offers a developed adsorbent that has the advantage of being recyclable, low cost, biodegradable and biocompatible for effectively removing PAHs from aqueous solution.					
32163776	3	53	theme	typical	678:684	arg1	pollutants					693:702	three typical target pollutants	672:702	three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene)	672:788	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	54	dep	hydrocarbons	727:738	arg1	fluorene					762:769	fluorene	762:769	fluorene	762:769	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	54	dep	hydrocarbons	727:738	arg1	phenanthrene					776:787	phenanthrene	776:787	phenanthrene	776:787	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	54	dep	hydrocarbons	727:738	arg1	acenaphthene					748:759	acenaphthene	748:759	acenaphthene	748:759	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
32163776	3	54	dep	hydrocarbons	727:738	arg1	hydrocarbons					727:738	polycyclic aromatic hydrocarbons	707:738	polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene)	707:788	The morphological properties of the composites were characterized thoroughly and employed in three typical target pollutants of polycyclic aromatic hydrocarbons (PAHs) (acenaphthene, fluorene, and phenanthrene) by batch adsorption experiments from aqueous solution.					
33811671	6	0	theme	/NG/cellulose	1018:1030	arg1	composite					1032:1040	NiFe2 O4 /NG/cellulose composite	1009:1040	NiFe2 O4 /NG/cellulose composite (0-50 μg/mL)	1009:1053	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	6	0	theme	/NG/cellulose	1018:1030	arg1	μg/mL					1048:1052	0-50 μg/mL	1043:1052	0-50 μg/mL	1043:1052	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	5	1	theme	particles	818:826	arg1	nature					804:809	the face-centered-cubic nature	780:809	the face-centered-cubic nature of the particles	780:826	The X-ray diffraction pattern showed the face-centered-cubic nature of the particles.					
33811671	3	2	theme	O4	519:520	arg1	-nanoparticles					522:535	NiFe2 O4 -nanoparticles	513:535	NiFe2 O4 -nanoparticles	513:535	The results showed the successful formation of NiFe2 O4 -nanoparticles with a spherical shape and a size ranging from 15 to 200 nm.					
33811671	6	3	theme	NiFe2	1009:1013	arg1	composite					1032:1040	NiFe2 O4 /NG/cellulose composite	1009:1040	NiFe2 O4 /NG/cellulose composite (0-50 μg/mL)	1009:1053	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	6	3	theme	NiFe2	1009:1013	arg1	μg/mL					1048:1052	0-50 μg/mL	1043:1052	0-50 μg/mL	1043:1052	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	5	4	theme	X-ray	747:751	arg1	pattern					765:771	The X-ray diffraction pattern	743:771	The X-ray diffraction pattern	743:771	The X-ray diffraction pattern showed the face-centered-cubic nature of the particles.					
33811671	1	5	theme	NiFe2	152:156	arg1	/NG/cellulose					222:234	NiFe2 O4 /NG/cellulose	213:234	NiFe2 O4 /NG/cellulose	213:234	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	5	theme	NiFe2	152:156	arg1	composite					202:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite	134:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose)	134:235	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	7	6	theme	O4	1126:1127	arg1	/NG/cellulose					1129:1141	NiFe2 O4 /NG/cellulose	1120:1141	NiFe2 O4 /NG/cellulose	1120:1141	Inhibitory activity increased with increasing concentrations of NiFe2 O4 /NG/cellulose.					
33811671	0	7	theme	bacterial	99:107	arg1	pathogens					109:117	bacterial pathogens	99:117	bacterial pathogens	99:117	Chemical synthesis of NiFe2 O4 /NG/cellulose nanocomposite and its antibacterial potential against bacterial pathogens.					
33811671	6	8	theme	Gram-negative	891:903	arg1	coli					885:888	Escherichia coli	873:888	Escherichia coli (Gram-negative bacteria)	873:913	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	6	8	theme	Gram-negative	891:903	arg1	bacteria					905:912	Gram-negative bacteria	891:912	Gram-negative bacteria	891:912	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	7	9	theme	/NG/cellulose	1129:1141	arg1	concentrations					1102:1115	increasing concentrations	1091:1115	increasing concentrations of NiFe2 O4 /NG/cellulose	1091:1141	Inhibitory activity increased with increasing concentrations of NiFe2 O4 /NG/cellulose.					
33811671	6	10	theme	different	981:989	arg1	concentrations					991:1004	different concentrations	981:1004	different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL)	981:1053	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	9	11	theme	synthesized	1314:1324	arg1	composite					1349:1357	the chemically synthesized NiFe2 O4 /NG/cellulose composite	1299:1357	the chemically synthesized NiFe2 O4 /NG/cellulose composite	1299:1357	Overall, the chemically synthesized NiFe2 O4 /NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.					
33811671	3	12	theme	spherical	544:552	arg1	shape					554:558	a spherical shape	542:558	a spherical shape	542:558	The results showed the successful formation of NiFe2 O4 -nanoparticles with a spherical shape and a size ranging from 15 to 200 nm.					
33811671	1	13	theme	/nitrogen-doped-graphene	162:185	arg1	/NG/cellulose					222:234	NiFe2 O4 /NG/cellulose	213:234	NiFe2 O4 /NG/cellulose	213:234	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	13	theme	/nitrogen-doped-graphene	162:185	arg1	composite					202:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite	134:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose)	134:235	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	14	theme	O4	158:159	arg1	/NG/cellulose					222:234	NiFe2 O4 /NG/cellulose	213:234	NiFe2 O4 /NG/cellulose	213:234	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	14	theme	O4	158:159	arg1	composite					202:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite	134:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose)	134:235	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	9	15	theme	pathogenic	1443:1452	arg1	bacteria					1454:1461	pathogenic bacteria	1443:1461	pathogenic bacteria	1443:1461	Overall, the chemically synthesized NiFe2 O4 /NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.					
33811671	9	16	theme	NiFe2	1326:1330	arg1	composite					1349:1357	the chemically synthesized NiFe2 O4 /NG/cellulose composite	1299:1357	the chemically synthesized NiFe2 O4 /NG/cellulose composite	1299:1357	Overall, the chemically synthesized NiFe2 O4 /NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.					
33811671	7	17	theme	NiFe2	1120:1124	arg1	/NG/cellulose					1129:1141	NiFe2 O4 /NG/cellulose	1120:1141	NiFe2 O4 /NG/cellulose	1120:1141	Inhibitory activity increased with increasing concentrations of NiFe2 O4 /NG/cellulose.					
33811671	4	18	theme	Energy-dispersive	598:614	arg1	results					622:628	Energy-dispersive X-ray results	598:628	Energy-dispersive X-ray results	598:628	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	3	19	dep	200	590:592	arg1	to					587:588	to	587:588	to	587:588	The results showed the successful formation of NiFe2 O4 -nanoparticles with a spherical shape and a size ranging from 15 to 200 nm.					
33811671	5	20	theme	face-centered-cubic	784:802	arg1	nature					804:809	the face-centered-cubic nature	780:809	the face-centered-cubic nature of the particles	780:826	The X-ray diffraction pattern showed the face-centered-cubic nature of the particles.					
33811671	8	21	theme	inhibitory	1160:1169	arg1	activity					1171:1178	The composite's inhibitory activity	1144:1178	The composite's inhibitory activity	1144:1178	The composite's inhibitory activity was slightly higher in E. coli than in B. subtilis due to the differing nature of their cell wall structures.					
33811671	8	21	theme	inhibitory	1160:1169	arg1	higher					1193:1198	higher	1193:1198	higher	1193:1198	The composite's inhibitory activity was slightly higher in E. coli than in B. subtilis due to the differing nature of their cell wall structures.					
33811671	7	22	theme	increasing	1091:1100	arg1	concentrations					1102:1115	increasing concentrations	1091:1115	increasing concentrations of NiFe2 O4 /NG/cellulose	1091:1141	Inhibitory activity increased with increasing concentrations of NiFe2 O4 /NG/cellulose.					
33811671	2	23	theme	/NG/cellulose	371:383	arg1	composite					385:393	The synthesized NiFe2 O4 /NG/cellulose composite	346:393	The synthesized NiFe2 O4 /NG/cellulose composite	346:393	The synthesized NiFe2 O4 /NG/cellulose composite was characterized by performing morphological and structural analyses.					
33811671	0	24	theme	Chemical	0:7	arg1	synthesis					9:17	Chemical synthesis	0:17	Chemical synthesis of NiFe2 O4	0:29	Chemical synthesis of NiFe2 O4 /NG/cellulose nanocomposite and its antibacterial potential against bacterial pathogens.					
33811671	9	25	theme	/NG/cellulose	1335:1347	arg1	composite					1349:1357	the chemically synthesized NiFe2 O4 /NG/cellulose composite	1299:1357	the chemically synthesized NiFe2 O4 /NG/cellulose composite	1299:1357	Overall, the chemically synthesized NiFe2 O4 /NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.					
33811671	8	26	theme	differing	1242:1250	arg1	nature					1252:1257	the differing nature	1238:1257	the differing nature of their cell wall structures	1238:1287	The composite's inhibitory activity was slightly higher in E. coli than in B. subtilis due to the differing nature of their cell wall structures.					
33811671	2	27	theme	O4	368:369	arg1	composite					385:393	The synthesized NiFe2 O4 /NG/cellulose composite	346:393	The synthesized NiFe2 O4 /NG/cellulose composite	346:393	The synthesized NiFe2 O4 /NG/cellulose composite was characterized by performing morphological and structural analyses.					
33811671	0	28	theme	NiFe2	22:26	arg1	O4					28:29	NiFe2 O4	22:29	NiFe2 O4	22:29	Chemical synthesis of NiFe2 O4 /NG/cellulose nanocomposite and its antibacterial potential against bacterial pathogens.					
33811671	9	29	theme	bacteria	1454:1461	arg1	growth					1433:1438	the growth	1429:1438	the growth of pathogenic bacteria	1429:1461	Overall, the chemically synthesized NiFe2 O4 /NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.					
33811671	4	30	theme	reaction	725:732	arg1	mixture					734:740	the reaction mixture	721:740	the reaction mixture	721:740	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	2	31	theme	morphological	427:439	arg1	analyses					456:463	morphological and structural analyses	427:463	morphological and structural analyses	427:463	The synthesized NiFe2 O4 /NG/cellulose composite was characterized by performing morphological and structural analyses.					
33811671	1	32	theme	facile	276:281	arg1	route					292:296	a facile chemical route	274:296	a facile chemical route	274:296	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	8	33	theme	cell	1268:1271	arg1	structures					1278:1287	their cell wall structures	1262:1287	their cell wall structures	1262:1287	The composite's inhibitory activity was slightly higher in E. coli than in B. subtilis due to the differing nature of their cell wall structures.					
33811671	3	34	theme	-nanoparticles	522:535	arg1	formation					500:508	the successful formation	485:508	the successful formation of NiFe2 O4 -nanoparticles	485:535	The results showed the successful formation of NiFe2 O4 -nanoparticles with a spherical shape and a size ranging from 15 to 200 nm.					
33811671	4	35	from	presence	644:651	arg1	mixture					734:740	the reaction mixture	721:740	the reaction mixture	721:740	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	1	36	theme	nickel	136:141	arg1	/NG/cellulose					222:234	NiFe2 O4 /NG/cellulose	213:234	NiFe2 O4 /NG/cellulose	213:234	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	36	theme	nickel	136:141	arg1	composite					202:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite	134:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose)	134:235	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	5	37	theme	diffraction	753:763	arg1	pattern					765:771	The X-ray diffraction pattern	743:771	The X-ray diffraction pattern	743:771	The X-ray diffraction pattern showed the face-centered-cubic nature of the particles.					
33811671	1	38	theme	chemical	283:290	arg1	route					292:296	a facile chemical route	274:296	a facile chemical route	274:296	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	7	39	theme	Inhibitory	1056:1065	arg1	activity					1067:1074	Inhibitory activity	1056:1074	Inhibitory activity	1056:1074	Inhibitory activity increased with increasing concentrations of NiFe2 O4 /NG/cellulose.					
33811671	4	40	theme	various	656:662	arg1	nickel					710:715	nickel	710:715	nickel	710:715	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	40	theme	various	656:662	arg1	nitrogen					682:689	nitrogen	682:689	nitrogen	682:689	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	40	theme	various	656:662	arg1	oxygen					692:697	oxygen	692:697	oxygen	692:697	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	40	theme	various	656:662	arg1	elements					664:671	various elements	656:671	various elements (carbon, nitrogen, oxygen, iron, and nickel)	656:716	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	40	theme	various	656:662	arg1	carbon					674:679	carbon	674:679	carbon	674:679	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	40	theme	various	656:662	arg1	iron					700:703	iron	700:703	iron	700:703	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	1	41	theme	/cellulose	191:200	arg1	/NG/cellulose					222:234	NiFe2 O4 /NG/cellulose	213:234	NiFe2 O4 /NG/cellulose	213:234	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	41	theme	/cellulose	191:200	arg1	composite					202:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite	134:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose)	134:235	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	0	42	theme	O4	28:29	arg1	synthesis					9:17	Chemical synthesis	0:17	Chemical synthesis of NiFe2 O4	0:29	Chemical synthesis of NiFe2 O4 /NG/cellulose nanocomposite and its antibacterial potential against bacterial pathogens.					
33811671	6	43	theme	antibacterial	842:854	arg1	activity					856:863	antibacterial activity	842:863	antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria)	842:960	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	2	44	theme	structural	445:454	arg1	analyses					456:463	morphological and structural analyses	427:463	morphological and structural analyses	427:463	The synthesized NiFe2 O4 /NG/cellulose composite was characterized by performing morphological and structural analyses.					
33811671	8	45	theme	wall	1273:1276	arg1	structures					1278:1287	their cell wall structures	1262:1287	their cell wall structures	1262:1287	The composite's inhibitory activity was slightly higher in E. coli than in B. subtilis due to the differing nature of their cell wall structures.					
33811671	6	46	theme	Gram-positive	938:950	arg1	subtilis					928:935	Bacillus subtilis	919:935	Bacillus subtilis (Gram-positive bacteria)	919:960	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	6	46	theme	Gram-positive	938:950	arg1	bacteria					952:959	Gram-positive bacteria	938:959	Gram-positive bacteria	938:959	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	9	47	contain	has	1359:1361	arg1	composite					1349:1357	the chemically synthesized NiFe2 O4 /NG/cellulose composite	1299:1357	the chemically synthesized NiFe2 O4 /NG/cellulose composite	1299:1357	Overall, the chemically synthesized NiFe2 O4 /NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.					
33811671	9	47	contain	has	1359:1361	arg2	potential					1367:1375	the potential	1363:1375	the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria	1363:1461	Overall, the chemically synthesized NiFe2 O4 /NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.					
33811671	3	48	theme	NiFe2	513:517	arg1	-nanoparticles					522:535	NiFe2 O4 -nanoparticles	513:535	NiFe2 O4 -nanoparticles	513:535	The results showed the successful formation of NiFe2 O4 -nanoparticles with a spherical shape and a size ranging from 15 to 200 nm.					
33811671	9	49	theme	efficient	1383:1391	arg1	agent					1407:1411	an efficient antibacterial agent	1380:1411	an efficient antibacterial agent for controlling the growth of pathogenic bacteria	1380:1461	Overall, the chemically synthesized NiFe2 O4 /NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.					
33811671	8	50	theme	structures	1278:1287	arg1	nature					1252:1257	the differing nature	1238:1257	the differing nature of their cell wall structures	1238:1287	The composite's inhibitory activity was slightly higher in E. coli than in B. subtilis due to the differing nature of their cell wall structures.					
33811671	4	51	attach	presence	644:651	arg2	nitrogen					682:689	nitrogen	682:689	nitrogen	682:689	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	51	attach	presence	644:651	arg2	elements					664:671	various elements	656:671	various elements (carbon, nitrogen, oxygen, iron, and nickel)	656:716	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	51	attach	presence	644:651	arg1	mixture					734:740	the reaction mixture	721:740	the reaction mixture	721:740	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	51	attach	presence	644:651	arg2	oxygen					692:697	oxygen	692:697	oxygen	692:697	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	51	attach	presence	644:651	arg2	carbon					674:679	carbon	674:679	carbon	674:679	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	51	attach	presence	644:651	arg2	iron					700:703	iron	700:703	iron	700:703	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	51	attach	presence	644:651	arg2	nickel					710:715	nickel	710:715	nickel	710:715	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	6	52	theme	composite	1032:1040	arg1	concentrations					991:1004	different concentrations	981:1004	different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL)	981:1053	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	3	53	theme	successful	489:498	arg1	formation					500:508	the successful formation	485:508	the successful formation of NiFe2 O4 -nanoparticles	485:535	The results showed the successful formation of NiFe2 O4 -nanoparticles with a spherical shape and a size ranging from 15 to 200 nm.					
33811671	9	54	theme	antibacterial	1393:1405	arg1	agent					1407:1411	an efficient antibacterial agent	1380:1411	an efficient antibacterial agent for controlling the growth of pathogenic bacteria	1380:1461	Overall, the chemically synthesized NiFe2 O4 /NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.					
33811671	4	55	theme	X-ray	616:620	arg1	results					622:628	Energy-dispersive X-ray results	598:628	Energy-dispersive X-ray results	598:628	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	2	56	theme	synthesized	350:360	arg1	composite					385:393	The synthesized NiFe2 O4 /NG/cellulose composite	346:393	The synthesized NiFe2 O4 /NG/cellulose composite	346:393	The synthesized NiFe2 O4 /NG/cellulose composite was characterized by performing morphological and structural analyses.					
33811671	1	57	theme	NiFe2	213:217	arg1	/NG/cellulose					222:234	NiFe2 O4 /NG/cellulose	213:234	NiFe2 O4 /NG/cellulose	213:234	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	57	theme	NiFe2	213:217	arg1	composite					202:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite	134:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose)	134:235	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	0	58	theme	antibacterial	67:79	arg1	potential					81:89	its antibacterial potential	63:89	its antibacterial potential against bacterial pathogens	63:117	Chemical synthesis of NiFe2 O4 /NG/cellulose nanocomposite and its antibacterial potential against bacterial pathogens.					
33811671	9	59	theme	O4	1332:1333	arg1	composite					1349:1357	the chemically synthesized NiFe2 O4 /NG/cellulose composite	1299:1357	the chemically synthesized NiFe2 O4 /NG/cellulose composite	1299:1357	Overall, the chemically synthesized NiFe2 O4 /NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.					
33811671	1	60	theme	NG	188:189	arg1	/NG/cellulose					222:234	NiFe2 O4 /NG/cellulose	213:234	NiFe2 O4 /NG/cellulose	213:234	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	60	theme	NG	188:189	arg1	composite					202:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite	134:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose)	134:235	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	2	61	theme	NiFe2	362:366	arg1	composite					385:393	The synthesized NiFe2 O4 /NG/cellulose composite	346:393	The synthesized NiFe2 O4 /NG/cellulose composite	346:393	The synthesized NiFe2 O4 /NG/cellulose composite was characterized by performing morphological and structural analyses.					
33811671	4	62	theme	elements	664:671	arg1	presence					644:651	the presence	640:651	the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture	640:740	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	1	63	theme	O4	219:220	arg1	/NG/cellulose					222:234	NiFe2 O4 /NG/cellulose	213:234	NiFe2 O4 /NG/cellulose	213:234	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	63	theme	O4	219:220	arg1	composite					202:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite	134:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose)	134:235	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	64	theme	antibacterial	307:319	arg1	potential					321:329	its antibacterial potential	303:329	its antibacterial potential	303:329	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	4	65	dep	elements	664:671	arg1	nickel					710:715	nickel	710:715	nickel	710:715	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	65	dep	elements	664:671	arg1	nitrogen					682:689	nitrogen	682:689	nitrogen	682:689	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	65	dep	elements	664:671	arg1	oxygen					692:697	oxygen	692:697	oxygen	692:697	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	65	dep	elements	664:671	arg1	elements					664:671	various elements	656:671	various elements (carbon, nitrogen, oxygen, iron, and nickel)	656:716	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	65	dep	elements	664:671	arg1	carbon					674:679	carbon	674:679	carbon	674:679	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	4	65	dep	elements	664:671	arg1	iron					700:703	iron	700:703	iron	700:703	Energy-dispersive X-ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture.					
33811671	1	66	theme	ferrite	143:149	arg1	/NG/cellulose					222:234	NiFe2 O4 /NG/cellulose	213:234	NiFe2 O4 /NG/cellulose	213:234	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	1	66	theme	ferrite	143:149	arg1	composite					202:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite	134:210	a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose)	134:235	In this work, a nickel ferrite (NiFe2 O4 )/nitrogen-doped-graphene (NG)/cellulose composite (NiFe2 O4 /NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated.					
33811671	6	67	theme	O4	1015:1016	arg1	composite					1032:1040	NiFe2 O4 /NG/cellulose composite	1009:1040	NiFe2 O4 /NG/cellulose composite (0-50 μg/mL)	1009:1053	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33811671	6	67	theme	O4	1015:1016	arg1	μg/mL					1048:1052	0-50 μg/mL	1043:1052	0-50 μg/mL	1043:1052	In addition, antibacterial activity against Escherichia coli (Gram-negative bacteria) and Bacillus subtilis (Gram-positive bacteria) was evaluated with different concentrations of NiFe2 O4 /NG/cellulose composite (0-50 μg/mL).					
33536563	7	0	from	methodology	1086:1096	arg1	biology					1106:1112	cell biology	1101:1112	cell biology	1101:1112	We anticipate our approach for modeling glycation in vivo will be a foundational methodology in cell biology.					
33536563	2	1	used	used	257:260	arg2	we					254:255	we	254:255	we	254:255	In our study we used in vitro generated AGEs to model glycation in vivo.					
33536563	8	2	theme	MAGE	1160:1163	arg1	discovery					1147:1155	the discovery	1143:1155	the discovery of MAGE	1143:1163	Further studies relevant to the discovery of MAGE may contribute to clarifying disease mechanisms and to the development of novel therapeutic options for diabetic complications, neuropathology, and cancer.					
33536563	5	3	attach	present	851:857	arg1	tissues					862:868	tissues	862:868	tissues	862:868	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	3	attach	present	851:857	arg2	product					799:805	the native and previously undescribed analogous glycation product	741:805	the native and previously undescribed analogous glycation product	741:805	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	3	attach	present	851:857	arg2	present					851:857	present	851:857	present	851:857	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	3	attach	present	851:857	arg1	humans					919:924	humans	919:924	humans	919:924	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	3	4	theme	in	328:329	arg1	analogs					336:342	in vivo analogs	328:342	in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin	328:495	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	7	5	theme	cell	1101:1104	arg1	biology					1106:1112	cell biology	1101:1112	cell biology	1101:1112	We anticipate our approach for modeling glycation in vivo will be a foundational methodology in cell biology.					
33536563	5	6	from	tissues	862:868	arg1	product					799:805	the native and previously undescribed analogous glycation product	741:805	the native and previously undescribed analogous glycation product	741:805	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	6	from	tissues	862:868	arg1	present					851:857	present	851:857	present	851:857	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	2	7	theme	generated	271:279	arg1	AGEs					281:284	in vitro generated AGEs	262:284	in vitro generated AGEs	262:284	In our study we used in vitro generated AGEs to model glycation in vivo.					
33536563	0	8	link	melibiose-derived	4:20	arg1	product					32:38	The melibiose-derived glycation product	0:38	The melibiose-derived glycation product	0:38	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	4	9	dep	identified	552:561	arg1	Using					498:502	Using	498:502	Using nuclear magnetic resonance spectroscopy	498:542	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	5	10	theme	analogous	779:787	arg1	present					851:857	present	851:857	present	851:857	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	10	theme	analogous	779:787	arg1	product					799:805	the native and previously undescribed analogous glycation product	741:805	the native and previously undescribed analogous glycation product	741:805	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	8	11	theme	therapeutic	1245:1255	arg1	options					1257:1263	novel therapeutic options	1239:1263	novel therapeutic options for diabetic complications, neuropathology, and cancer	1239:1318	Further studies relevant to the discovery of MAGE may contribute to clarifying disease mechanisms and to the development of novel therapeutic options for diabetic complications, neuropathology, and cancer.					
33536563	5	12	theme	undescribed	767:777	arg1	present					851:857	present	851:857	present	851:857	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	12	theme	undescribed	767:777	arg1	product					799:805	the native and previously undescribed analogous glycation product	741:805	the native and previously undescribed analogous glycation product	741:805	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	13	theme	invertebrates	878:890	arg1	tissues					862:868	tissues	862:868	tissues	862:868	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	13	theme	invertebrates	878:890	arg1	humans					919:924	humans	919:924	humans	919:924	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	14	theme	glycation	789:797	arg1	present					851:857	present	851:857	present	851:857	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	14	theme	glycation	789:797	arg1	product					799:805	the native and previously undescribed analogous glycation product	741:805	the native and previously undescribed analogous glycation product	741:805	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	3	15	theme	unusual	347:353	arg1	melibiose-adducts					355:371	unusual melibiose-adducts	347:371	unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin	347:495	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	6	16	from	auto-antibodies	962:976	arg1	patients					981:988	patients	981:988	patients with diabetes	981:1002	We also report MAGE cross-reactive auto-antibodies in patients with diabetes.					
33536563	8	17	theme	relevant	1131:1138	arg1	studies					1123:1129	Further studies	1115:1129	Further studies relevant to the discovery of MAGE	1115:1163	Further studies relevant to the discovery of MAGE may contribute to clarifying disease mechanisms and to the development of novel therapeutic options for diabetic complications, neuropathology, and cancer.					
33536563	4	18	theme	moiety	649:654	arg1	set					574:576	a set	572:576	a set	572:576	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	4	18	theme	moiety	649:654	arg1	MAGEs					563:567	MAGEs	563:567	MAGEs	563:567	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	4	18	theme	moiety	649:654	arg1	isomers					581:587	isomers	581:587	isomers	581:587	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	4	18	theme	moiety	649:654	arg1	moiety					649:654	the fructosamine moiety	632:654	the fructosamine moiety	632:654	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	3	19	theme	melibiose-adducts	355:371	arg1	analogs					336:342	in vivo analogs	328:342	in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin	328:495	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	7	20	theme	foundational	1073:1084	arg1	methodology					1086:1096	a foundational methodology	1071:1096	a foundational methodology	1071:1096	We anticipate our approach for modeling glycation in vivo will be a foundational methodology in cell biology.					
33536563	7	20	theme	foundational	1073:1084	arg1	approach					1023:1030	our approach	1019:1030	our approach for modeling glycation in vivo	1019:1061	We anticipate our approach for modeling glycation in vivo will be a foundational methodology in cell biology.					
33536563	0	21	dep	human	75:79	arg1	tissues					92:98	tissues	92:98	tissues	92:98	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	8	22	theme	options	1257:1263	arg1	development					1224:1234	the development	1220:1234	the development of novel therapeutic options for diabetic complications, neuropathology, and cancer	1220:1318	Further studies relevant to the discovery of MAGE may contribute to clarifying disease mechanisms and to the development of novel therapeutic options for diabetic complications, neuropathology, and cancer.					
33536563	4	23	theme	fructosamine	636:647	arg1	moiety					649:654	the fructosamine moiety	632:654	the fructosamine moiety	632:654	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	8	24	theme	disease	1194:1200	arg1	mechanisms					1202:1211	clarifying disease mechanisms	1183:1211	clarifying disease mechanisms	1183:1211	Further studies relevant to the discovery of MAGE may contribute to clarifying disease mechanisms and to the development of novel therapeutic options for diabetic complications, neuropathology, and cancer.					
33536563	6	25	theme	cross-reactive	947:960	arg1	auto-antibodies					962:976	MAGE cross-reactive auto-antibodies	942:976	MAGE cross-reactive auto-antibodies in patients with diabetes	942:1002	We also report MAGE cross-reactive auto-antibodies in patients with diabetes.					
33536563	5	26	theme	mouse	672:676	arg1	antibody					699:706	a mouse anti-MAGE monoclonal antibody	670:706	a mouse anti-MAGE monoclonal antibody	670:706	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	4	27	with	isomers	581:587	arg1	structures					617:626	cyclic structures	610:626	cyclic structures	610:626	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	4	27	with	isomers	581:587	arg1	open-chain					595:604	open-chain	595:604	open-chain	595:604	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	3	28	theme	anhydrous	436:444	arg1	conditions					446:455	anhydrous conditions	436:455	anhydrous conditions with bovine serum albumin and myoglobin	436:495	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	0	29	theme	glycation	22:30	arg1	product					32:38	The melibiose-derived glycation product	0:38	The melibiose-derived glycation product	0:38	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	6	30	theme	MAGE	942:945	arg1	auto-antibodies					962:976	MAGE cross-reactive auto-antibodies	942:976	MAGE cross-reactive auto-antibodies in patients with diabetes	942:1002	We also report MAGE cross-reactive auto-antibodies in patients with diabetes.					
33536563	5	31	theme	anti-MAGE	678:686	arg1	antibody					699:706	a mouse anti-MAGE monoclonal antibody	670:706	a mouse anti-MAGE monoclonal antibody	670:706	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	1	32	theme	Non-enzymatic	101:113	arg1	modification					115:126	Non-enzymatic modification	101:126	Non-enzymatic modification	101:126	Non-enzymatic modification of proteins by carbohydrates, known as glycation, leads to generation of advanced glycation end-products (AGEs).					
33536563	5	33	theme	living	817:822	arg1	organisms					824:832	living organisms	817:832	living organisms	817:832	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	34	theme	native	745:750	arg1	present					851:857	present	851:857	present	851:857	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	34	theme	native	745:750	arg1	product					799:805	the native and previously undescribed analogous glycation product	741:805	the native and previously undescribed analogous glycation product	741:805	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	35	theme	monoclonal	688:697	arg1	antibody					699:706	a mouse anti-MAGE monoclonal antibody	670:706	a mouse anti-MAGE monoclonal antibody	670:706	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	0	36	from	human	75:79	arg1	present					64:70	present	64:70	present	64:70	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	8	37	theme	novel	1239:1243	arg1	options					1257:1263	novel therapeutic options	1239:1263	novel therapeutic options for diabetic complications, neuropathology, and cancer	1239:1318	Further studies relevant to the discovery of MAGE may contribute to clarifying disease mechanisms and to the development of novel therapeutic options for diabetic complications, neuropathology, and cancer.					
33536563	1	38	theme	advanced	201:208	arg1	AGEs					234:237	AGEs	234:237	AGEs	234:237	Non-enzymatic modification of proteins by carbohydrates, known as glycation, leads to generation of advanced glycation end-products (AGEs).					
33536563	1	38	theme	advanced	201:208	arg1	end-products					220:231	advanced glycation end-products	201:231	advanced glycation end-products (AGEs)	201:238	Non-enzymatic modification of proteins by carbohydrates, known as glycation, leads to generation of advanced glycation end-products (AGEs).					
33536563	3	39	theme	mel-derived	391:401	arg1	MAGEs					384:388	MAGEs	384:388	MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin	384:495	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	3	39	theme	mel-derived	391:401	arg1	AGEs					403:406	mel-derived AGEs	391:406	mel-derived AGEs	391:406	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	4	40	theme	magnetic	512:519	arg1	resonance					521:529	nuclear magnetic resonance	504:529	nuclear magnetic resonance spectroscopy	504:542	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	3	41	with	conditions	446:455	arg1	myoglobin					487:495	myoglobin	487:495	myoglobin	487:495	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	3	41	with	conditions	446:455	arg1	albumin					475:481	bovine serum albumin	462:481	bovine serum albumin	462:481	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	0	42	attach	present	64:70	arg2	epitope					56:62	a unique epitope	47:62	a unique epitope present in human and animal tissues	47:98	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	0	42	attach	present	64:70	arg1	human					75:79	human	75:79	human	75:79	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	0	42	attach	present	64:70	arg1	animal					85:90	animal	85:90	animal	85:90	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	1	43	theme	glycation	210:218	arg1	AGEs					234:237	AGEs	234:237	AGEs	234:237	Non-enzymatic modification of proteins by carbohydrates, known as glycation, leads to generation of advanced glycation end-products (AGEs).					
33536563	1	43	theme	glycation	210:218	arg1	end-products					220:231	advanced glycation end-products	201:231	advanced glycation end-products (AGEs)	201:238	Non-enzymatic modification of proteins by carbohydrates, known as glycation, leads to generation of advanced glycation end-products (AGEs).					
33536563	4	44	theme	nuclear	504:510	arg1	resonance					521:529	nuclear magnetic resonance	504:529	nuclear magnetic resonance spectroscopy	504:542	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	7	45	theme	modeling	1036:1043	arg1	glycation					1045:1053	modeling glycation	1036:1053	modeling glycation	1036:1053	We anticipate our approach for modeling glycation in vivo will be a foundational methodology in cell biology.					
33536563	0	46	from	present	64:70	arg1	human					75:79	human	75:79	human	75:79	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	0	46	from	present	64:70	arg1	animal					85:90	animal	85:90	animal	85:90	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	8	47	theme	Further	1115:1121	arg1	studies					1123:1129	Further studies	1115:1129	Further studies relevant to the discovery of MAGE	1115:1163	Further studies relevant to the discovery of MAGE may contribute to clarifying disease mechanisms and to the development of novel therapeutic options for diabetic complications, neuropathology, and cancer.					
33536563	3	48	theme	serum	469:473	arg1	albumin					475:481	bovine serum albumin	462:481	bovine serum albumin	462:481	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	0	49	theme	unique	49:54	arg1	epitope					56:62	a unique epitope	47:62	a unique epitope present in human and animal tissues	47:98	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	3	50	link	mel-derived	391:401	arg1	MAGEs					384:388	MAGEs	384:388	MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin	384:495	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	3	50	link	mel-derived	391:401	arg1	AGEs					403:406	mel-derived AGEs	391:406	mel-derived AGEs	391:406	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	5	51	theme	vertebrates	896:906	arg1	tissues					862:868	tissues	862:868	tissues	862:868	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	51	theme	vertebrates	896:906	arg1	humans					919:924	humans	919:924	humans	919:924	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	52	located	present	851:857	arg1	tissues					862:868	tissues	862:868	tissues	862:868	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	52	located	present	851:857	arg2	product					799:805	the native and previously undescribed analogous glycation product	741:805	the native and previously undescribed analogous glycation product	741:805	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	52	located	present	851:857	arg2	present					851:857	present	851:857	present	851:857	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	52	located	present	851:857	arg1	humans					919:924	humans	919:924	humans	919:924	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	4	53	theme	resonance	521:529	arg1	spectroscopy					531:542	nuclear magnetic resonance spectroscopy	504:542	nuclear magnetic resonance spectroscopy	504:542	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	3	54	dep	in	328:329	arg1	vivo					331:334	vivo	331:334	vivo	331:334	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	6	55	with	patients	981:988	arg1	diabetes					995:1002	diabetes	995:1002	diabetes	995:1002	We also report MAGE cross-reactive auto-antibodies in patients with diabetes.					
33536563	1	56	theme	proteins	131:138	arg1	modification					115:126	Non-enzymatic modification	101:126	Non-enzymatic modification	101:126	Non-enzymatic modification of proteins by carbohydrates, known as glycation, leads to generation of advanced glycation end-products (AGEs).					
33536563	1	57	theme	end-products	220:231	arg1	generation					187:196	generation	187:196	generation of advanced glycation end-products (AGEs)	187:238	Non-enzymatic modification of proteins by carbohydrates, known as glycation, leads to generation of advanced glycation end-products (AGEs).					
33536563	5	58	from	present	851:857	arg1	tissues					862:868	tissues	862:868	tissues	862:868	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	5	58	from	present	851:857	arg1	humans					919:924	humans	919:924	humans	919:924	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	3	59	theme	bovine	462:467	arg1	albumin					475:481	bovine serum albumin	462:481	bovine serum albumin	462:481	We discovered in vivo analogs of unusual melibiose-adducts designated MAGEs (mel-derived AGEs) synthesized in vitro under anhydrous conditions with bovine serum albumin and myoglobin.					
33536563	4	60	theme	isomers	581:587	arg1	set					574:576	a set	572:576	a set	572:576	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	4	60	theme	isomers	581:587	arg1	MAGEs					563:567	MAGEs	563:567	MAGEs	563:567	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	4	60	theme	isomers	581:587	arg1	isomers					581:587	isomers	581:587	isomers	581:587	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	4	60	theme	isomers	581:587	arg1	moiety					649:654	the fructosamine moiety	632:654	the fructosamine moiety	632:654	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	5	61	theme	first	725:729	arg1	time					731:734	the first time	721:734	the first time	721:734	We generated a mouse anti-MAGE monoclonal antibody and show for the first time that the native and previously undescribed analogous glycation product exists in living organisms and is naturally present in tissues of both invertebrates and vertebrates, including humans.					
33536563	0	62	from	animal	85:90	arg1	present					64:70	present	64:70	present	64:70	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	8	63	theme	diabetic	1269:1276	arg1	complications					1278:1290	diabetic complications	1269:1290	diabetic complications	1269:1290	Further studies relevant to the discovery of MAGE may contribute to clarifying disease mechanisms and to the development of novel therapeutic options for diabetic complications, neuropathology, and cancer.					
33536563	4	64	theme	cyclic	610:615	arg1	structures					617:626	cyclic structures	610:626	cyclic structures	610:626	Using nuclear magnetic resonance spectroscopy we have identified MAGEs as a set of isomers, with open-chain and cyclic structures, of the fructosamine moiety.					
33536563	0	65	theme	present	64:70	arg1	epitope					56:62	a unique epitope	47:62	a unique epitope present in human and animal tissues	47:98	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
33536563	8	66	theme	clarifying	1183:1192	arg1	mechanisms					1202:1211	clarifying disease mechanisms	1183:1211	clarifying disease mechanisms	1183:1211	Further studies relevant to the discovery of MAGE may contribute to clarifying disease mechanisms and to the development of novel therapeutic options for diabetic complications, neuropathology, and cancer.					
33536563	0	67	theme	melibiose-derived	4:20	arg1	product					32:38	The melibiose-derived glycation product	0:38	The melibiose-derived glycation product	0:38	The melibiose-derived glycation product mimics a unique epitope present in human and animal tissues.					
32438110	14	0	theme	dityrosine	2289:2298	arg1	linkages					2300:2307	dityrosine linkages	2289:2307	dityrosine linkages	2289:2307	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	2	1	theme	organic	327:333	arg1	exoskeleton					335:345	an organic exoskeleton	324:345	an organic exoskeleton	324:345	Ascidians (sea squirts) produce an organic exoskeleton, known as a tunic, which has been studied quite extensively in several species.					
32438110	16	2	theme	advanced	2493:2500	arg1	materials					2502:2510	advanced materials	2493:2510	advanced materials	2493:2510	Given current efforts to utilize cellulose to produce advanced materials, the tunic of the sea pineapple provides a striking model for the design of bio-inspired cellulosic composites.					
32438110	3	3	theme	detailed	495:502	arg1	structure					504:512	detailed structure	495:512	detailed structure	495:512	However, currently, there are still gaps in our knowledge about the detailed structure and composition of this cellulosic biocomposite.					
32438110	6	4	with	layer	1095:1099	arg1	composition					1115:1125	elevated composition	1106:1125	elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages	1106:1212	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	6	5	theme	dityrosine	1194:1203	arg1	linkages					1205:1212	dityrosine linkages	1194:1212	dityrosine linkages	1194:1212	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	16	6	theme	pineapple	2534:2542	arg1	tunic					2517:2521	the tunic	2513:2521	the tunic of the sea pineapple	2513:2542	Given current efforts to utilize cellulose to produce advanced materials, the tunic of the sea pineapple provides a striking model for the design of bio-inspired cellulosic composites.					
32438110	4	7	theme	electron	843:850	arg1	microscopies					852:863	electron microscopies	843:863	electron microscopies	843:863	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	4	8	theme	tough	634:638	arg1	tunic					640:644	the tough tunic	630:644	the tough tunic from the species Halocynthia roretzi	630:681	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	5	9	with	composite	950:958	arg1	zones					1041:1045	several compositionally and structurally distinct zones	991:1045	several compositionally and structurally distinct zones	991:1045	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	16	10	theme	current	2445:2451	arg1	efforts					2453:2459	current efforts	2445:2459	current efforts to utilize cellulose to produce advanced materials	2445:2510	Given current efforts to utilize cellulose to produce advanced materials, the tunic of the sea pineapple provides a striking model for the design of bio-inspired cellulosic composites.					
32438110	0	11	theme	composite	102:110	arg1	biomaterial					112:122	A complex cellulosic composite biomaterial	81:122	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.	0:123	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	4	12	from	composition	588:598	arg1	species					655:661	the species Halocynthia roretzi	651:681	the species Halocynthia roretzi	651:681	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	9	13	theme	cellulose	1518:1526	arg1	sources					1528:1534	biorenewable cellulose sources	1505:1534	biorenewable cellulose sources for the sustainable production of bio-inspired composites	1505:1592	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	15	14	theme	cellulose	2392:2400	arg1	fibres					2402:2407	well-ordered cellulose fibres	2379:2407	well-ordered cellulose fibres with a lower protein content	2379:2436	The fibrous layer makes up the bulk of the tunic and is comprised of well-ordered cellulose fibres with a lower protein content.					
32438110	4	15	theme	hierarchical	604:615	arg1	structure					617:625	hierarchical structure	604:625	hierarchical structure	604:625	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	5	16	with	proteins	977:984	arg1	zones					1041:1045	several compositionally and structurally distinct zones	991:1045	several compositionally and structurally distinct zones	991:1045	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	12	17	theme	cross-disciplinary	2028:2045	arg1	approach					2047:2054	a multiscale cross-disciplinary approach	2015:2054	a multiscale cross-disciplinary approach	2015:2054	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	15	18	theme	lower	2416:2420	arg1	content					2430:2436	a lower protein content	2414:2436	a lower protein content	2414:2436	The fibrous layer makes up the bulk of the tunic and is comprised of well-ordered cellulose fibres with a lower protein content.					
32438110	3	19	theme	cellulosic	538:547	arg1	biocomposite					549:560	this cellulosic biocomposite	533:560	this cellulosic biocomposite	533:560	However, currently, there are still gaps in our knowledge about the detailed structure and composition of this cellulosic biocomposite.					
32438110	9	20	theme	sustainable	1544:1554	arg1	production					1556:1565	the sustainable production	1540:1565	the sustainable production of bio-inspired composites	1540:1592	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	9	21	theme	roretzi	1636:1642	arg1	archetype					1663:1671	an exciting new archetype	1647:1671	an exciting new archetype for extracting relevant design principles	1647:1713	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	9	21	theme	roretzi	1636:1642	arg1	tunic					1624:1628	the tunic	1620:1628	the tunic of H. roretzi	1620:1642	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	5	22	theme	roretzi	911:917	arg1	cellulose					963:971	cellulose	963:971	cellulose	963:971	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	5	22	theme	roretzi	911:917	arg1	composite					950:958	a hierarchically-structured composite	922:958	a hierarchically-structured composite of cellulose	922:971	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	5	22	theme	roretzi	911:917	arg1	tunic					899:903	the tunic	895:903	the tunic of H. roretzi	895:917	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	16	23	theme	cellulosic	2601:2610	arg1	composites					2612:2621	bio-inspired cellulosic composites	2588:2621	bio-inspired cellulosic composites	2588:2621	Given current efforts to utilize cellulose to produce advanced materials, the tunic of the sea pineapple provides a striking model for the design of bio-inspired cellulosic composites.					
32438110	0	24	theme	sea	46:48	arg1	roretzi					72:78	the sea pineapple Halocynthia roretzi	42:78	the sea pineapple Halocynthia roretzi	42:78	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	13	25	theme	distinct	2133:2140	arg1	layers					2142:2147	two distinct layers	2129:2147	two distinct layers	2129:2147	The tunic of this species is a composite of cellulose and proteins with two distinct layers.					
32438110	9	26	theme	composites	1583:1592	arg1	production					1556:1565	the sustainable production	1540:1565	the sustainable production of bio-inspired composites	1540:1592	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	0	27	from	composition	14:24	arg1	roretzi					72:78	the sea pineapple Halocynthia roretzi	42:78	the sea pineapple Halocynthia roretzi	42:78	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	16	28	theme	sea	2530:2532	arg1	pineapple					2534:2542	the sea pineapple	2526:2542	the sea pineapple	2526:2542	Given current efforts to utilize cellulose to produce advanced materials, the tunic of the sea pineapple provides a striking model for the design of bio-inspired cellulosic composites.					
32438110	0	29	theme	Halocynthia	60:70	arg1	roretzi					72:78	the sea pineapple Halocynthia roretzi	42:78	the sea pineapple Halocynthia roretzi	42:78	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	12	30	theme	sea	1973:1975	arg1	roretzi					1999:2005	the sea pineapple Halocynthia roretzi	1969:2005	the sea pineapple Halocynthia roretzi	1969:2005	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	1	31	theme	composite	171:179	arg1	materials					181:189	high-performance composite materials	154:189	high-performance composite materials	154:189	Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials.					
32438110	1	31	theme	composite	171:179	arg1	cuticle					222:228	bone, wood and insect cuticle	200:228	cuticle	222:228	Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials.					
32438110	0	32	from	Structure	0:8	arg1	roretzi					72:78	the sea pineapple Halocynthia roretzi	42:78	the sea pineapple Halocynthia roretzi	42:78	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	14	33	theme	sclerotized	2175:2185	arg1	layer					2197:2201	a thin sclerotized cuticular layer	2168:2201	a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages	2168:2307	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	12	34	theme	Halocynthia	1987:1997	arg1	roretzi					1999:2005	the sea pineapple Halocynthia roretzi	1969:2005	the sea pineapple Halocynthia roretzi	1969:2005	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	5	35	theme	cellulose	963:971	arg1	cellulose					963:971	cellulose	963:971	cellulose	963:971	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	5	35	theme	cellulose	963:971	arg1	composite					950:958	a hierarchically-structured composite	922:958	a hierarchically-structured composite of cellulose	922:971	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	5	35	theme	cellulose	963:971	arg1	tunic					899:903	the tunic	895:903	the tunic of H. roretzi	895:917	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	5	35	theme	cellulose	963:971	arg1	proteins					977:984	proteins	977:984	proteins with several compositionally and structurally distinct zones	977:1045	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	14	36	attach	cross-linked	2272:2283	arg3	linkages					2300:2307	dityrosine linkages	2289:2307	dityrosine linkages	2289:2307	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	1	37	theme	Biological	125:134	arg1	organisms					136:144	Biological organisms	125:144	Biological organisms	125:144	Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials.					
32438110	0	38	theme	complex	83:89	arg1	biomaterial					112:122	A complex cellulosic composite biomaterial	81:122	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.	0:123	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	12	39	theme	tough	1952:1956	arg1	tunic					1958:1962	the tough tunic	1948:1962	the tough tunic	1948:1962	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	11	40	theme	structural	1815:1824	arg1	polysaccharide					1826:1839	this structural polysaccharide	1810:1839	this structural polysaccharide	1810:1839	They use this structural polysaccharide to build an exoskeleton called a tunic.					
32438110	6	41	theme	sclerotized	1073:1083	arg1	layer					1095:1099	a thin sclerotized cuticular layer	1066:1099	a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages	1066:1212	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	2	42	theme	several	410:416	arg1	species					418:424	several species	410:424	several species	410:424	Ascidians (sea squirts) produce an organic exoskeleton, known as a tunic, which has been studied quite extensively in several species.					
32438110	4	43	theme	cross-disciplinary	694:711	arg1	approach					713:720	a cross-disciplinary approach	692:720	a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies	692:863	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	9	44	theme	H.	1633:1634	arg1	roretzi					1636:1642	H. roretzi	1633:1642	H. roretzi	1633:1642	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	7	45	theme	lower	1351:1355	arg1	content					1365:1371	a lower protein content	1349:1371	a lower protein content	1349:1371	The fibrous layer makes up the bulk of the tunic and is comprised primarily of helicoidally-ordered crystalline cellulose fibres with a lower protein content.					
32438110	1	46	theme	novel	275:279	arg1	materials					281:289	novel materials	275:289	novel materials	275:289	Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials.					
32438110	12	47	theme	hierarchical	1922:1933	arg1	structure					1935:1943	hierarchical structure	1922:1943	hierarchical structure	1922:1943	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	16	48	theme	striking	2555:2562	arg1	model					2564:2568	a striking model	2553:2568	a striking model for the design of bio-inspired cellulosic composites	2553:2621	Given current efforts to utilize cellulose to produce advanced materials, the tunic of the sea pineapple provides a striking model for the design of bio-inspired cellulosic composites.					
32438110	3	49	dep	structure	504:512	arg1	the					491:493	the	491:493	the	491:493	However, currently, there are still gaps in our knowledge about the detailed structure and composition of this cellulosic biocomposite.					
32438110	14	50	dep	content	2225:2231	arg1	cross-linked					2272:2283	cross-linked	2272:2283	cross-linked via dityrosine linkages	2272:2307	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	14	50	dep	content	2225:2231	arg1	containing					2233:2242	containing	2233:2242	containing halogenated amino acids	2233:2266	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	9	51	theme	exciting	1650:1657	arg1	tunic					1624:1628	the tunic	1620:1628	the tunic of H. roretzi	1620:1642	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	9	51	theme	exciting	1650:1657	arg1	archetype					1663:1671	an exciting new archetype	1647:1671	an exciting new archetype for extracting relevant design principles	1647:1713	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	7	52	theme	cellulose	1327:1335	arg1	fibres					1337:1342	helicoidally-ordered crystalline cellulose fibres	1294:1342	helicoidally-ordered crystalline cellulose fibres with a lower protein content	1294:1371	The fibrous layer makes up the bulk of the tunic and is comprised primarily of helicoidally-ordered crystalline cellulose fibres with a lower protein content.					
32438110	8	53	contain	contains	1425:1432	arg2	fibres					1464:1469	much less organized cellulose fibres	1434:1469	much less organized cellulose fibres	1434:1469	The subcuticular zone directly beneath the surface contains much less organized cellulose fibres.					
32438110	8	53	contain	contains	1425:1432	arg1	zone					1391:1394	The subcuticular zone	1374:1394	The subcuticular zone directly beneath the surface	1374:1423	The subcuticular zone directly beneath the surface contains much less organized cellulose fibres.					
32438110	15	54	theme	protein	2422:2428	arg1	content					2430:2436	a lower protein content	2414:2436	a lower protein content	2414:2436	The fibrous layer makes up the bulk of the tunic and is comprised of well-ordered cellulose fibres with a lower protein content.					
32438110	8	55	theme	organized	1444:1452	arg1	fibres					1464:1469	much less organized cellulose fibres	1434:1469	much less organized cellulose fibres	1434:1469	The subcuticular zone directly beneath the surface contains much less organized cellulose fibres.					
32438110	10	56	theme	able	1774:1777	arg1	animals					1766:1772	the only animals	1757:1772	the only animals able to produce cellulose	1757:1798	STATEMENT OF SIGNIFICANCE: Tunicates are the only animals able to produce cellulose.					
32438110	10	56	theme	able	1774:1777	arg1	STATEMENT					1716:1724	STATEMENT	1716:1724	STATEMENT OF SIGNIFICANCE: Tunicates	1716:1751	STATEMENT OF SIGNIFICANCE: Tunicates are the only animals able to produce cellulose.					
32438110	15	57	theme	fibrous	2314:2320	arg1	layer					2322:2326	The fibrous layer	2310:2326	The fibrous layer	2310:2326	The fibrous layer makes up the bulk of the tunic and is comprised of well-ordered cellulose fibres with a lower protein content.					
32438110	16	58	theme	composites	2612:2621	arg1	design					2578:2583	the design	2574:2583	the design of bio-inspired cellulosic composites	2574:2621	Given current efforts to utilize cellulose to produce advanced materials, the tunic of the sea pineapple provides a striking model for the design of bio-inspired cellulosic composites.					
32438110	7	59	theme	helicoidally-ordered	1294:1313	arg1	fibres					1337:1342	helicoidally-ordered crystalline cellulose fibres	1294:1342	helicoidally-ordered crystalline cellulose fibres with a lower protein content	1294:1371	The fibrous layer makes up the bulk of the tunic and is comprised primarily of helicoidally-ordered crystalline cellulose fibres with a lower protein content.					
32438110	6	60	dep	protein	1130:1136	arg1	cross-linked					1177:1188	cross-linked	1177:1188	cross-linked via dityrosine linkages	1177:1212	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	6	60	dep	protein	1130:1136	arg1	containing					1138:1147	containing	1138:1147	containing halogenated amino acids	1138:1171	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	5	61	theme	distinct	1032:1039	arg1	zones					1041:1045	several compositionally and structurally distinct zones	991:1045	several compositionally and structurally distinct zones	991:1045	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	10	62	theme	only	1761:1764	arg1	animals					1766:1772	the only animals	1757:1772	the only animals able to produce cellulose	1757:1798	STATEMENT OF SIGNIFICANCE: Tunicates are the only animals able to produce cellulose.					
32438110	10	62	theme	only	1761:1764	arg1	STATEMENT					1716:1724	STATEMENT	1716:1724	STATEMENT OF SIGNIFICANCE: Tunicates	1716:1751	STATEMENT OF SIGNIFICANCE: Tunicates are the only animals able to produce cellulose.					
32438110	1	63	theme	insect	215:220	arg1	cuticle					222:228	bone, wood and insect cuticle	200:228	cuticle	222:228	Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials.					
32438110	16	64	theme	bio-inspired	2588:2599	arg1	composites					2612:2621	bio-inspired cellulosic composites	2588:2621	bio-inspired cellulosic composites	2588:2621	Given current efforts to utilize cellulose to produce advanced materials, the tunic of the sea pineapple provides a striking model for the design of bio-inspired cellulosic composites.					
32438110	5	65	theme	several	991:997	arg1	zones					1041:1045	several compositionally and structurally distinct zones	991:1045	several compositionally and structurally distinct zones	991:1045	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	10	66	theme	SIGNIFICANCE	1729:1740	arg1	STATEMENT					1716:1724	STATEMENT	1716:1724	STATEMENT OF SIGNIFICANCE: Tunicates	1716:1751	STATEMENT OF SIGNIFICANCE: Tunicates are the only animals able to produce cellulose.					
32438110	10	66	theme	SIGNIFICANCE	1729:1740	arg1	animals					1766:1772	the only animals	1757:1772	the only animals able to produce cellulose	1757:1798	STATEMENT OF SIGNIFICANCE: Tunicates are the only animals able to produce cellulose.					
32438110	9	67	theme	design	1697:1702	arg1	principles					1704:1713	relevant design principles	1688:1713	relevant design principles	1688:1713	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	6	68	theme	elevated	1106:1113	arg1	composition					1115:1125	elevated composition	1106:1125	elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages	1106:1212	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	15	69	with	fibres	2402:2407	arg1	content					2430:2436	a lower protein content	2414:2436	a lower protein content	2414:2436	The fibrous layer makes up the bulk of the tunic and is comprised of well-ordered cellulose fibres with a lower protein content.					
32438110	7	70	theme	tunic	1258:1262	arg1	bulk					1246:1249	the bulk	1242:1249	the bulk of the tunic	1242:1262	The fibrous layer makes up the bulk of the tunic and is comprised primarily of helicoidally-ordered crystalline cellulose fibres with a lower protein content.					
32438110	3	71	from	gaps	463:466	arg1	knowledge					475:483	our knowledge	471:483	our knowledge about the detailed structure and composition of this cellulosic biocomposite	471:560	However, currently, there are still gaps in our knowledge about the detailed structure and composition of this cellulosic biocomposite.					
32438110	15	72	theme	tunic	2353:2357	arg1	bulk					2341:2344	the bulk	2337:2344	the bulk of the tunic	2337:2357	The fibrous layer makes up the bulk of the tunic and is comprised of well-ordered cellulose fibres with a lower protein content.					
32438110	14	73	from	surface	2157:2163	arg1	layer					2197:2201	a thin sclerotized cuticular layer	2168:2201	a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages	2168:2307	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	13	74	with	composite	2088:2096	arg1	layers					2142:2147	two distinct layers	2129:2147	two distinct layers	2129:2147	The tunic of this species is a composite of cellulose and proteins with two distinct layers.					
32438110	9	75	theme	current	1478:1484	arg1	efforts					1486:1492	current efforts	1478:1492	current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites	1478:1592	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	4	76	theme	atomic	826:831	arg1	force					833:837	atomic force	826:837	atomic force	826:837	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	14	77	theme	halogenated	2244:2254	arg1	acids					2262:2266	halogenated amino acids	2244:2266	halogenated amino acids	2244:2266	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	14	78	theme	amino	2256:2260	arg1	acids					2262:2266	halogenated amino acids	2244:2266	halogenated amino acids	2244:2266	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	2	79	theme	sea	303:305	arg1	Ascidians					292:300	Ascidians	292:300	Ascidians (sea squirts)	292:314	Ascidians (sea squirts) produce an organic exoskeleton, known as a tunic, which has been studied quite extensively in several species.					
32438110	2	79	theme	sea	303:305	arg1	squirts					307:313	sea squirts	303:313	sea squirts	303:313	Ascidians (sea squirts) produce an organic exoskeleton, known as a tunic, which has been studied quite extensively in several species.					
32438110	4	80	from	structure	617:625	arg1	species					655:661	the species Halocynthia roretzi	651:681	the species Halocynthia roretzi	651:681	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	6	81	from	surface	1055:1061	arg1	layer					1095:1099	a thin sclerotized cuticular layer	1066:1099	a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages	1066:1212	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	1	82	theme	bone	200:203	arg1	cuticle					222:228	bone, wood and insect cuticle	200:228	cuticle	222:228	Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials.					
32438110	0	83	from	roretzi	72:78	arg1	composition					14:24	composition	14:24	composition	14:24	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	0	83	from	roretzi	72:78	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	6	84	theme	amino	1161:1165	arg1	acids					1167:1171	halogenated amino acids	1149:1171	halogenated amino acids	1149:1171	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	14	85	theme	protein	2217:2223	arg1	content					2225:2231	a higher protein content	2208:2231	a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages	2208:2307	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	7	86	with	fibres	1337:1342	arg1	content					1365:1371	a lower protein content	1349:1371	a lower protein content	1349:1371	The fibrous layer makes up the bulk of the tunic and is comprised primarily of helicoidally-ordered crystalline cellulose fibres with a lower protein content.					
32438110	3	87	theme	biocomposite	549:560	arg1	composition					518:528	composition	518:528	composition	518:528	However, currently, there are still gaps in our knowledge about the detailed structure and composition of this cellulosic biocomposite.					
32438110	3	87	theme	biocomposite	549:560	arg1	structure					504:512	detailed structure	495:512	detailed structure	495:512	However, currently, there are still gaps in our knowledge about the detailed structure and composition of this cellulosic biocomposite.					
32438110	1	88	theme	wood	206:209	arg1	cuticle					222:228	bone, wood and insect cuticle	200:228	cuticle	222:228	Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials.					
32438110	12	89	theme	multiscale	2017:2026	arg1	approach					2047:2054	a multiscale cross-disciplinary approach	2015:2054	a multiscale cross-disciplinary approach	2015:2054	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	4	90	theme	vibrational	777:787	arg1	spectroscopy					789:800	vibrational spectroscopy	777:800	vibrational spectroscopy	777:800	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	12	91	from	roretzi	1999:2005	arg1	composition					1906:1916	composition	1906:1916	composition	1906:1916	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	12	91	from	roretzi	1999:2005	arg1	structure					1935:1943	hierarchical structure	1922:1943	hierarchical structure	1922:1943	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	13	92	theme	cellulose	2101:2109	arg1	composite					2088:2096	a composite	2086:2096	a composite of cellulose	2086:2109	The tunic of this species is a composite of cellulose and proteins with two distinct layers.					
32438110	13	92	theme	cellulose	2101:2109	arg1	cellulose					2101:2109	cellulose	2101:2109	cellulose	2101:2109	The tunic of this species is a composite of cellulose and proteins with two distinct layers.					
32438110	13	92	theme	cellulose	2101:2109	arg1	proteins					2115:2122	proteins	2115:2122	proteins with two distinct layers	2115:2147	The tunic of this species is a composite of cellulose and proteins with two distinct layers.					
32438110	13	92	theme	cellulose	2101:2109	arg1	tunic					2061:2065	The tunic	2057:2065	The tunic of this species	2057:2081	The tunic of this species is a composite of cellulose and proteins with two distinct layers.					
32438110	6	93	attach	cross-linked	1177:1188	arg3	linkages					1205:1212	dityrosine linkages	1194:1212	dityrosine linkages	1194:1212	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	0	94	theme	pineapple	50:58	arg1	roretzi					72:78	the sea pineapple Halocynthia roretzi	42:78	the sea pineapple Halocynthia roretzi	42:78	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	9	95	theme	biorenewable	1505:1516	arg1	sources					1528:1534	biorenewable cellulose sources	1505:1534	biorenewable cellulose sources for the sustainable production of bio-inspired composites	1505:1592	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	9	96	theme	bio-inspired	1570:1581	arg1	composites					1583:1592	bio-inspired composites	1570:1592	bio-inspired composites	1570:1592	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	13	97	with	proteins	2115:2122	arg1	layers					2142:2147	two distinct layers	2129:2147	two distinct layers	2129:2147	The tunic of this species is a composite of cellulose and proteins with two distinct layers.					
32438110	4	98	theme	tunic	640:644	arg1	composition					588:598	composition	588:598	composition	588:598	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	4	98	theme	tunic	640:644	arg1	structure					617:625	hierarchical structure	604:625	hierarchical structure	604:625	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	12	99	theme	pineapple	1977:1985	arg1	roretzi					1999:2005	the sea pineapple Halocynthia roretzi	1969:2005	the sea pineapple Halocynthia roretzi	1969:2005	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	16	100	dep	efforts	2453:2459	arg1	utilize					2464:2470	utilize	2464:2470	to utilize cellulose to produce advanced materials	2461:2510	Given current efforts to utilize cellulose to produce advanced materials, the tunic of the sea pineapple provides a striking model for the design of bio-inspired cellulosic composites.					
32438110	1	101	theme	high-performance	154:169	arg1	materials					181:189	high-performance composite materials	154:189	high-performance composite materials	154:189	Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials.					
32438110	1	101	theme	high-performance	154:169	arg1	cuticle					222:228	bone, wood and insect cuticle	200:228	cuticle	222:228	Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials.					
32438110	14	102	theme	cuticular	2187:2195	arg1	layer					2197:2201	a thin sclerotized cuticular layer	2168:2201	a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages	2168:2307	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	4	103	dep	species	655:661	arg1	roretzi					675:681	roretzi	675:681	roretzi	675:681	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	14	104	theme	thin	2170:2173	arg1	layer					2197:2201	a thin sclerotized cuticular layer	2168:2201	a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages	2168:2307	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	4	105	theme	traditional	732:742	arg1	histology					744:752	traditional histology	732:752	traditional histology	732:752	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	0	106	theme	cellulosic	91:100	arg1	biomaterial					112:122	A complex cellulosic composite biomaterial	81:122	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.	0:123	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	12	107	theme	tunic	1958:1962	arg1	composition					1906:1916	composition	1906:1916	composition	1906:1916	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	12	107	theme	tunic	1958:1962	arg1	structure					1935:1943	hierarchical structure	1922:1943	hierarchical structure	1922:1943	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	6	108	theme	cuticular	1085:1093	arg1	layer					1095:1099	a thin sclerotized cuticular layer	1066:1099	a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages	1066:1212	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	0	109	from	tunic	33:37	arg1	roretzi					72:78	the sea pineapple Halocynthia roretzi	42:78	the sea pineapple Halocynthia roretzi	42:78	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	8	110	theme	subcuticular	1378:1389	arg1	zone					1391:1394	The subcuticular zone	1374:1394	The subcuticular zone directly beneath the surface	1374:1423	The subcuticular zone directly beneath the surface contains much less organized cellulose fibres.					
32438110	6	111	theme	thin	1068:1071	arg1	layer					1095:1099	a thin sclerotized cuticular layer	1066:1099	a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages	1066:1212	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	5	112	theme	hierarchically-structured	924:948	arg1	cellulose					963:971	cellulose	963:971	cellulose	963:971	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	5	112	theme	hierarchically-structured	924:948	arg1	composite					950:958	a hierarchically-structured composite	922:958	a hierarchically-structured composite of cellulose	922:971	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	5	112	theme	hierarchically-structured	924:948	arg1	tunic					899:903	the tunic	895:903	the tunic of H. roretzi	895:917	The picture emerging is that the tunic of H. roretzi is a hierarchically-structured composite of cellulose and proteins with several compositionally and structurally distinct zones.					
32438110	0	113	theme	tunic	33:37	arg1	composition					14:24	composition	14:24	composition	14:24	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	0	113	theme	tunic	33:37	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	1	114	theme	materials	281:289	arg1	design					265:270	the design	261:270	the design of novel materials	261:289	Biological organisms produce high-performance composite materials, such as bone, wood and insect cuticle, which provide inspiration for the design of novel materials.					
32438110	7	115	theme	crystalline	1315:1325	arg1	fibres					1337:1342	helicoidally-ordered crystalline cellulose fibres	1294:1342	helicoidally-ordered crystalline cellulose fibres with a lower protein content	1294:1371	The fibrous layer makes up the bulk of the tunic and is comprised primarily of helicoidally-ordered crystalline cellulose fibres with a lower protein content.					
32438110	7	116	theme	protein	1357:1363	arg1	content					1365:1371	a lower protein content	1349:1371	a lower protein content	1349:1371	The fibrous layer makes up the bulk of the tunic and is comprised primarily of helicoidally-ordered crystalline cellulose fibres with a lower protein content.					
32438110	14	117	theme	higher	2210:2215	arg1	content					2225:2231	a higher protein content	2208:2231	a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages	2208:2307	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	4	118	theme	X-ray	803:807	arg1	diffraction					809:819	X-ray diffraction	803:819	X-ray diffraction	803:819	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	7	119	theme	fibrous	1219:1225	arg1	layer					1227:1231	The fibrous layer	1215:1231	The fibrous layer	1215:1231	The fibrous layer makes up the bulk of the tunic and is comprised primarily of helicoidally-ordered crystalline cellulose fibres with a lower protein content.					
32438110	4	120	dep	composition	588:598	arg1	the					584:586	the	584:586	the	584:586	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	6	121	theme	halogenated	1149:1159	arg1	acids					1167:1171	halogenated amino acids	1149:1171	halogenated amino acids	1149:1171	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	9	122	dep	efforts	1486:1492	arg1	utilize					1497:1503	utilize	1497:1503	to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites	1494:1592	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	14	123	with	layer	2197:2201	arg1	content					2225:2231	a higher protein content	2208:2231	a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages	2208:2307	At the surface is a thin sclerotized cuticular layer with a higher protein content containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	0	124	dep	Structure	0:8	arg1	biomaterial					112:122	A complex cellulosic composite biomaterial	81:122	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.	0:123	Structure and composition of the tunic in the sea pineapple Halocynthia roretzi: A complex cellulosic composite biomaterial.					
32438110	9	125	theme	new	1659:1661	arg1	tunic					1624:1628	the tunic	1620:1628	the tunic of H. roretzi	1620:1642	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	9	125	theme	new	1659:1661	arg1	archetype					1663:1671	an exciting new archetype	1647:1671	an exciting new archetype for extracting relevant design principles	1647:1713	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	6	126	theme	protein	1130:1136	arg1	composition					1115:1125	elevated composition	1106:1125	elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages	1106:1212	At the surface is a thin sclerotized cuticular layer with elevated composition of protein containing halogenated amino acids and cross-linked via dityrosine linkages.					
32438110	8	127	theme	cellulose	1454:1462	arg1	fibres					1464:1469	much less organized cellulose fibres	1434:1469	much less organized cellulose fibres	1434:1469	The subcuticular zone directly beneath the surface contains much less organized cellulose fibres.					
32438110	9	128	theme	relevant	1688:1695	arg1	principles					1704:1713	relevant design principles	1688:1713	relevant design principles	1688:1713	Given current efforts to utilize biorenewable cellulose sources for the sustainable production of bio-inspired composites, these insights establish the tunic of H. roretzi as an exciting new archetype for extracting relevant design principles.					
32438110	12	129	dep	composition	1906:1916	arg1	the					1902:1904	the	1902:1904	the	1902:1904	Here, we investigate the composition and hierarchical structure of the tough tunic from the sea pineapple Halocynthia roretzi through a multiscale cross-disciplinary approach.					
32438110	10	130	dep	STATEMENT	1716:1724	arg1	Tunicates					1743:1751	Tunicates	1743:1751	Tunicates	1743:1751	STATEMENT OF SIGNIFICANCE: Tunicates are the only animals able to produce cellulose.					
32438110	13	131	theme	species	2075:2081	arg1	composite					2088:2096	a composite	2086:2096	a composite of cellulose	2086:2109	The tunic of this species is a composite of cellulose and proteins with two distinct layers.					
32438110	13	131	theme	species	2075:2081	arg1	cellulose					2101:2109	cellulose	2101:2109	cellulose	2101:2109	The tunic of this species is a composite of cellulose and proteins with two distinct layers.					
32438110	13	131	theme	species	2075:2081	arg1	tunic					2061:2065	The tunic	2057:2065	The tunic of this species	2057:2081	The tunic of this species is a composite of cellulose and proteins with two distinct layers.					
32438110	4	132	from	species	655:661	arg1	tunic					640:644	the tough tunic	630:644	the tough tunic from the species Halocynthia roretzi	630:681	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	4	132	from	species	655:661	arg1	composition					588:598	composition	588:598	composition	588:598	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	4	132	from	species	655:661	arg1	structure					617:625	hierarchical structure	604:625	hierarchical structure	604:625	Here, we investigate the composition and hierarchical structure of the tough tunic from the species Halocynthia roretzi, through a cross-disciplinary approach combining traditional histology, immunohistochemistry, vibrational spectroscopy, X-ray diffraction, and atomic force and electron microscopies.					
32438110	15	133	theme	well-ordered	2379:2390	arg1	fibres					2402:2407	well-ordered cellulose fibres	2379:2407	well-ordered cellulose fibres with a lower protein content	2379:2436	The fibrous layer makes up the bulk of the tunic and is comprised of well-ordered cellulose fibres with a lower protein content.					
32991902	0	0	theme	low	84:86	arg1	costs					99:103	low production costs	84:103	low production costs	84:103	Biodegradable films based on commercial κ-carrageenan and cassava starch to achieve low production costs.					
32991902	12	1	theme	low-cost	1366:1373	arg1	films					1389:1393	low-cost biodegradable films	1366:1393	low-cost biodegradable films from commercial polysaccharides	1366:1425	We produced low-cost biodegradable films from commercial polysaccharides.					
32991902	4	2	theme	κ-carrageenan/cassava	618:638	arg1	ratios					654:659	different κ-carrageenan/cassava starch weight ratios	608:659	different κ-carrageenan/cassava starch weight ratios	608:659	For the first time, biodegradable films were produced by casting commercials kappa-carrageenan (κ-car) and cassava starch at different κ-carrageenan/cassava starch weight ratios.					
32991902	4	3	theme	commercials	548:558	arg1	κ-car					579:583	κ-car	579:583	κ-car	579:583	For the first time, biodegradable films were produced by casting commercials kappa-carrageenan (κ-car) and cassava starch at different κ-carrageenan/cassava starch weight ratios.					
32991902	4	3	theme	commercials	548:558	arg1	kappa-carrageenan					560:576	commercials kappa-carrageenan	548:576	commercials kappa-carrageenan (κ-car)	548:584	For the first time, biodegradable films were produced by casting commercials kappa-carrageenan (κ-car) and cassava starch at different κ-carrageenan/cassava starch weight ratios.					
32991902	4	4	theme	different	608:616	arg1	ratios					654:659	different κ-carrageenan/cassava starch weight ratios	608:659	different κ-carrageenan/cassava starch weight ratios	608:659	For the first time, biodegradable films were produced by casting commercials kappa-carrageenan (κ-car) and cassava starch at different κ-carrageenan/cassava starch weight ratios.					
32991902	3	5	theme	biodegradable	369:381	arg1	films					383:387	biodegradable films	369:387	biodegradable films	369:387	Indeed, biodegradable films can also reduce the environmental pollution promoted by non-biodegradable conventional packs.					
32991902	7	6	with	film	837:840	arg1	%					910:910	391.6%	905:910	391.6%	905:910	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	7	6	with	film	837:840	arg1	solubility					858:867	high water solubility	847:867	high water solubility (39.22%)	847:876	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	7	6	with	film	837:840	arg1	%					875:875	39.22%	870:875	39.22%	870:875	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	7	6	with	film	837:840	arg1	degree					897:902	a low swelling degree	882:902	a low swelling degree (391.6%)	882:911	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	2	7	theme	shelf	313:317	arg1	life					319:322	the foodstuff shelf life	299:322	the foodstuff shelf life	299:322	These properties may raise the foodstuff shelf life, reducing costs and economic losses.					
32991902	10	8	theme	0κ-c	1198:1201	arg1	films					1203:1207	the 25κ-c and 0κ-c films	1184:1207	the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively	1184:1270	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	4	9	theme	weight	647:652	arg1	ratios					654:659	different κ-carrageenan/cassava starch weight ratios	608:659	different κ-carrageenan/cassava starch weight ratios	608:659	For the first time, biodegradable films were produced by casting commercials kappa-carrageenan (κ-car) and cassava starch at different κ-carrageenan/cassava starch weight ratios.					
32991902	0	10	theme	production	88:97	arg1	costs					99:103	low production costs	84:103	low production costs	84:103	Biodegradable films based on commercial κ-carrageenan and cassava starch to achieve low production costs.					
32991902	4	11	theme	biodegradable	503:515	arg1	films					517:521	biodegradable films	503:521	biodegradable films	503:521	For the first time, biodegradable films were produced by casting commercials kappa-carrageenan (κ-car) and cassava starch at different κ-carrageenan/cassava starch weight ratios.					
32991902	5	12	theme	Physical	662:669	arg1	properties					696:705	Physical, thermal, and mechanical properties	662:705	Physical, thermal, and mechanical properties	662:705	Physical, thermal, and mechanical properties were evaluated.					
32991902	2	13	theme	foodstuff	303:311	arg1	life					319:322	the foodstuff shelf life	299:322	the foodstuff shelf life	299:322	These properties may raise the foodstuff shelf life, reducing costs and economic losses.					
32991902	7	14	theme	swelling	888:895	arg1	%					910:910	391.6%	905:910	391.6%	905:910	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	7	14	theme	swelling	888:895	arg1	degree					897:902	a low swelling degree	882:902	a low swelling degree (391.6%)	882:911	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	4	15	theme	starch	640:645	arg1	ratios					654:659	different κ-carrageenan/cassava starch weight ratios	608:659	different κ-carrageenan/cassava starch weight ratios	608:659	For the first time, biodegradable films were produced by casting commercials kappa-carrageenan (κ-car) and cassava starch at different κ-carrageenan/cassava starch weight ratios.					
32991902	9	16	theme	0.0043mmm2	1045:1054	arg1	d-1					1056:1058	0.0043mmm2 d-1	1045:1058	0.0043mmm2 d-1	1045:1058	The oil permeability varied from 0.0033 to 0.0043mmm2 d-1.					
32991902	5	17	theme	thermal	672:678	arg1	properties					696:705	Physical, thermal, and mechanical properties	662:705	Physical, thermal, and mechanical properties	662:705	Physical, thermal, and mechanical properties were evaluated.					
32991902	8	18	theme	3.01×10-8g	980:989	arg1	50κ-c					973:977	50κ-c	973:977	50κ-c (3.01×10-8g (Pams)-1)	973:999	The lowest water vapor permeability (WVP) was observed for 50κ-c (3.01×10-8g (Pams)-1).					
32991902	8	18	theme	3.01×10-8g	980:989	arg1	Pams					992:995	3.01×10-8g (Pams)-1	980:998	3.01×10-8g (Pams)-1	980:998	The lowest water vapor permeability (WVP) was observed for 50κ-c (3.01×10-8g (Pams)-1).					
32991902	1	19	theme	non-renewable	168:180	arg1	sources					182:188	non-renewable sources	168:188	non-renewable sources	168:188	Biodegradable films have been a great alternative compared to non-renewable sources because of their cytocompatibility, biodegradability, and antimicrobial features.					
32991902	11	20	theme	starch	1312:1317	arg1	concentration					1319:1331	the starch concentration	1308:1331	the starch concentration raised in the blend	1308:1351	The thermal stability increased as the starch concentration raised in the blend.					
32991902	10	21	theme	100κ-c	1065:1070	arg1	films					1082:1086	The 100κ-c and 75κ-c films	1061:1086	The 100κ-c and 75κ-c films (with high κ-carrageenan contents)	1061:1121	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	10	22	theme	κ-carrageenan	1099:1111	arg1	contents					1113:1120	high κ-carrageenan contents	1094:1120	high κ-carrageenan contents	1094:1120	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	8	23	theme	lowest	918:923	arg1	WVP					951:953	WVP	951:953	WVP	951:953	The lowest water vapor permeability (WVP) was observed for 50κ-c (3.01×10-8g (Pams)-1).					
32991902	8	23	theme	lowest	918:923	arg1	permeability					937:948	The lowest water vapor permeability	914:948	The lowest water vapor permeability (WVP)	914:954	The lowest water vapor permeability (WVP) was observed for 50κ-c (3.01×10-8g (Pams)-1).					
32991902	7	24	theme	low	884:886	arg1	%					910:910	391.6%	905:910	391.6%	905:910	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	7	24	theme	low	884:886	arg1	degree					897:902	a low swelling degree	882:902	a low swelling degree (391.6%)	882:911	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	3	25	theme	non-biodegradable	445:461	arg1	packs					476:480	non-biodegradable conventional packs	445:480	non-biodegradable conventional packs	445:480	Indeed, biodegradable films can also reduce the environmental pollution promoted by non-biodegradable conventional packs.					
32991902	0	26	theme	Biodegradable	0:12	arg1	films					14:18	Biodegradable films	0:18	Biodegradable films based on commercial κ-carrageenan and cassava starch	0:71	Biodegradable films based on commercial κ-carrageenan and cassava starch to achieve low production costs.					
32991902	4	27	theme	cassava	590:596	arg1	starch					598:603	cassava starch	590:603	cassava starch	590:603	For the first time, biodegradable films were produced by casting commercials kappa-carrageenan (κ-car) and cassava starch at different κ-carrageenan/cassava starch weight ratios.					
32991902	5	28	theme	mechanical	685:694	arg1	properties					696:705	Physical, thermal, and mechanical properties	662:705	Physical, thermal, and mechanical properties	662:705	Physical, thermal, and mechanical properties were evaluated.					
32991902	6	29	theme	color	744:748	arg1	analyses					750:757	Apparent opacity and color analyses	723:757	Apparent opacity and color analyses	723:757	Apparent opacity and color analyses suggest that the films present high transparency.					
32991902	3	30	theme	conventional	463:474	arg1	packs					476:480	non-biodegradable conventional packs	445:480	non-biodegradable conventional packs	445:480	Indeed, biodegradable films can also reduce the environmental pollution promoted by non-biodegradable conventional packs.					
32991902	10	31	theme	high	1094:1097	arg1	contents					1113:1120	high κ-carrageenan contents	1094:1120	high κ-carrageenan contents	1094:1120	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	8	32	theme	water	925:929	arg1	WVP					951:953	WVP	951:953	WVP	951:953	The lowest water vapor permeability (WVP) was observed for 50κ-c (3.01×10-8g (Pams)-1).					
32991902	8	32	theme	water	925:929	arg1	permeability					937:948	The lowest water vapor permeability	914:948	The lowest water vapor permeability (WVP)	914:954	The lowest water vapor permeability (WVP) was observed for 50κ-c (3.01×10-8g (Pams)-1).					
32991902	7	33	theme	high	847:850	arg1	solubility					858:867	high water solubility	847:867	high water solubility (39.22%)	847:876	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	7	33	theme	high	847:850	arg1	%					875:875	39.22%	870:875	39.22%	870:875	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	1	34	theme	Biodegradable	106:118	arg1	alternative					144:154	a great alternative	136:154	a great alternative compared to non-renewable sources	136:188	Biodegradable films have been a great alternative compared to non-renewable sources because of their cytocompatibility, biodegradability, and antimicrobial features.					
32991902	1	34	theme	Biodegradable	106:118	arg1	films					120:124	Biodegradable films	106:124	Biodegradable films	106:124	Biodegradable films have been a great alternative compared to non-renewable sources because of their cytocompatibility, biodegradability, and antimicrobial features.					
32991902	6	35	theme	opacity	732:738	arg1	analyses					750:757	Apparent opacity and color analyses	723:757	Apparent opacity and color analyses	723:757	Apparent opacity and color analyses suggest that the films present high transparency.					
32991902	11	36	theme	thermal	1277:1283	arg1	stability					1285:1293	The thermal stability	1273:1293	The thermal stability	1273:1293	The thermal stability increased as the starch concentration raised in the blend.					
32991902	7	37	theme	water	852:856	arg1	solubility					858:867	high water solubility	847:867	high water solubility (39.22%)	847:876	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	7	37	theme	water	852:856	arg1	%					875:875	39.22%	870:875	39.22%	870:875	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	10	38	from	break	1228:1232	arg1	elongation					1214:1223	elongation	1214:1223	elongation at break (ε) of 21.60 and 67.65%, respectively	1214:1270	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	6	39	theme	Apparent	723:730	arg1	analyses					750:757	Apparent opacity and color analyses	723:757	Apparent opacity and color analyses	723:757	Apparent opacity and color analyses suggest that the films present high transparency.					
32991902	10	40	with	films	1082:1086	arg1	contents					1113:1120	high κ-carrageenan contents	1094:1120	high κ-carrageenan contents	1094:1120	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	0	41	theme	commercial	29:38	arg1	κ-carrageenan					40:52	commercial κ-carrageenan	29:52	commercial κ-carrageenan	29:52	Biodegradable films based on commercial κ-carrageenan and cassava starch to achieve low production costs.					
32991902	10	42	theme	higher	1127:1132	arg1	stiffness					1134:1142	higher stiffness	1127:1142	higher stiffness (19.23 and 25.88MPa, respectively)	1127:1177	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	3	43	theme	environmental	409:421	arg1	pollution					423:431	the environmental pollution	405:431	the environmental pollution promoted by non-biodegradable conventional packs	405:480	Indeed, biodegradable films can also reduce the environmental pollution promoted by non-biodegradable conventional packs.					
32991902	10	44	with	films	1203:1207	arg1	elongation					1214:1223	elongation	1214:1223	elongation at break (ε) of 21.60 and 67.65%, respectively	1214:1270	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	4	45	theme	first	491:495	arg1	time					497:500	the first time	487:500	the first time	487:500	For the first time, biodegradable films were produced by casting commercials kappa-carrageenan (κ-car) and cassava starch at different κ-carrageenan/cassava starch weight ratios.					
32991902	8	46	theme	vapor	931:935	arg1	WVP					951:953	WVP	951:953	WVP	951:953	The lowest water vapor permeability (WVP) was observed for 50κ-c (3.01×10-8g (Pams)-1).					
32991902	8	46	theme	vapor	931:935	arg1	permeability					937:948	The lowest water vapor permeability	914:948	The lowest water vapor permeability (WVP)	914:954	The lowest water vapor permeability (WVP) was observed for 50κ-c (3.01×10-8g (Pams)-1).					
32991902	12	47	theme	biodegradable	1375:1387	arg1	films					1389:1393	low-cost biodegradable films	1366:1393	low-cost biodegradable films from commercial polysaccharides	1366:1425	We produced low-cost biodegradable films from commercial polysaccharides.					
32991902	12	48	theme	commercial	1400:1409	arg1	polysaccharides					1411:1425	commercial polysaccharides	1400:1425	commercial polysaccharides	1400:1425	We produced low-cost biodegradable films from commercial polysaccharides.					
32991902	10	49	theme	%	1256:1256	arg1	ε					1235:1235	ε	1235:1235	ε	1235:1235	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	10	49	theme	%	1256:1256	arg1	break					1228:1232	break	1228:1232	break (ε) of 21.60 and 67.65%, respectively	1228:1270	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	10	50	dep	stiffness	1134:1142	arg1	19.23					1145:1149	19.23	1145:1149	19.23	1145:1149	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	10	50	dep	stiffness	1134:1142	arg1	25.88MPa					1155:1162	25.88MPa	1155:1162	25.88MPa	1155:1162	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	2	51	theme	economic	344:351	arg1	losses					353:358	economic losses	344:358	economic losses	344:358	These properties may raise the foodstuff shelf life, reducing costs and economic losses.					
32991902	10	52	theme	25κ-c	1188:1192	arg1	films					1203:1207	the 25κ-c and 0κ-c films	1184:1207	the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively	1184:1270	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	7	53	theme	sample	813:818	arg1	0κ-c					820:823	The sample 0κ-c	809:823	The sample 0κ-c	809:823	The sample 0κ-c supported a film with high water solubility (39.22%) and a low swelling degree (391.6%).					
32991902	13	54	theme	food	1455:1458	arg1	packs					1460:1464	food packs	1455:1464	food packs	1455:1464	These films can be used as food packs.					
32991902	13	54	theme	food	1455:1458	arg1	films					1434:1438	These films	1428:1438	These films	1428:1438	These films can be used as food packs.					
32991902	13	55	used	used	1447:1450	arg2	films					1434:1438	These films	1428:1438	These films	1428:1438	These films can be used as food packs.					
32991902	13	55	used	used	1447:1450	arg2	packs					1460:1464	food packs	1455:1464	food packs	1455:1464	These films can be used as food packs.					
32991902	9	56	theme	oil	1006:1008	arg1	permeability					1010:1021	The oil permeability	1002:1021	The oil permeability	1002:1021	The oil permeability varied from 0.0033 to 0.0043mmm2 d-1.					
32991902	6	57	theme	high	790:793	arg1	transparency					795:806	high transparency	790:806	high transparency	790:806	Apparent opacity and color analyses suggest that the films present high transparency.					
32991902	1	58	theme	great	138:142	arg1	alternative					144:154	a great alternative	136:154	a great alternative compared to non-renewable sources	136:188	Biodegradable films have been a great alternative compared to non-renewable sources because of their cytocompatibility, biodegradability, and antimicrobial features.					
32991902	1	58	theme	great	138:142	arg1	films					120:124	Biodegradable films	106:124	Biodegradable films	106:124	Biodegradable films have been a great alternative compared to non-renewable sources because of their cytocompatibility, biodegradability, and antimicrobial features.					
32991902	12	59	from	polysaccharides	1411:1425	arg1	films					1389:1393	low-cost biodegradable films	1366:1393	low-cost biodegradable films from commercial polysaccharides	1366:1425	We produced low-cost biodegradable films from commercial polysaccharides.					
32991902	1	60	theme	antimicrobial	248:260	arg1	features					262:269	antimicrobial features	248:269	antimicrobial features	248:269	Biodegradable films have been a great alternative compared to non-renewable sources because of their cytocompatibility, biodegradability, and antimicrobial features.					
32991902	0	61	theme	cassava	58:64	arg1	starch					66:71	cassava starch	58:71	cassava starch	58:71	Biodegradable films based on commercial κ-carrageenan and cassava starch to achieve low production costs.					
32991902	10	62	theme	75κ-c	1076:1080	arg1	films					1082:1086	The 100κ-c and 75κ-c films	1061:1086	The 100κ-c and 75κ-c films (with high κ-carrageenan contents)	1061:1121	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	10	63	contain	had	1123:1125	arg2	stiffness					1134:1142	higher stiffness	1127:1142	higher stiffness (19.23 and 25.88MPa, respectively)	1127:1177	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
32991902	10	63	contain	had	1123:1125	arg1	films					1082:1086	The 100κ-c and 75κ-c films	1061:1086	The 100κ-c and 75κ-c films (with high κ-carrageenan contents)	1061:1121	The 100κ-c and 75κ-c films (with high κ-carrageenan contents) had higher stiffness (19.23 and 25.88MPa, respectively) than the 25κ-c and 0κ-c films with elongation at break (ε) of 21.60 and 67.65%, respectively.					
33676868	6	0	dep	Fourier	681:687	arg1	transform					689:697	transform	689:697	transform infrared spectroscopic analysis of SCTx	689:737	The Fourier transform infrared spectroscopic analysis of SCTx displayed the peaks corresponding to those obtained from commercial xylan.					
33676868	1	1	attach	present	131:137	arg2	Xylan					98:102	Xylan	98:102	Xylan	98:102	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	1	1	attach	present	131:137	arg1	walls					172:176	sugarcane stem secondary cell walls	142:176	sugarcane stem secondary cell walls	142:176	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	1	1	attach	present	131:137	arg2	hemicellulose					117:129	the major hemicellulose	107:129	the major hemicellulose present in sugarcane stem secondary cell walls	107:176	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	12	2	theme	polymerization	1701:1714	arg1	degree					1691:1696	degree	1691:1696	degree of polymerization 2-6	1691:1718	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	12	2	theme	polymerization	1701:1714	arg1	xylo-oligosaccharides					1739:1759	no substituted xylo-oligosaccharides	1724:1759	no substituted xylo-oligosaccharides because of the endolytic activity of enzyme	1724:1803	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	4	3	theme	%	524:524	arg1	presence					446:453	the presence	442:453	the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose	442:539	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	7	4	theme	FESEM	814:818	arg1	analysis					820:827	FESEM analysis	814:827	FESEM analysis of SCTx	814:835	FESEM analysis of SCTx showed the granular and porous surface structure.					
33676868	5	5	theme	size	559:562	arg1	analysis					590:597	High performance size exclusion chromatographic analysis	542:597	High performance size exclusion chromatographic analysis of SCTx	542:605	High performance size exclusion chromatographic analysis of SCTx displayed a single peak corresponding to a molecular mass of ∼57 kDa.					
33676868	8	6	theme	thermal	955:961	arg1	Td					989:990	Td	989:990	Td	989:990	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	8	6	theme	thermal	955:961	arg1	temperatures					975:986	two thermal degradation temperatures	951:986	two thermal degradation temperatures (Td) of 228°C	951:1000	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	5	7	theme	chromatographic	574:588	arg1	analysis					590:597	High performance size exclusion chromatographic analysis	542:597	High performance size exclusion chromatographic analysis of SCTx	542:605	High performance size exclusion chromatographic analysis of SCTx displayed a single peak corresponding to a molecular mass of ∼57 kDa.					
33676868	2	8	with	backbone	207:214	arg1	degree					228:233	a high degree	221:233	a high degree	221:233	Xylan is composed of xylose backbone with a high degree of substitutions, which affects its properties.					
33676868	12	9	theme	layer	1523:1527	arg1	chromatography					1529:1542	thin layer chromatography	1518:1542	thin layer chromatography	1518:1542	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	9	10	theme	acid	1161:1164	arg1	presence					1124:1131	The presence	1120:1131	The presence of arabinose and glucuronic acid as a side chains	1120:1181	The presence of arabinose and glucuronic acid as a side chains was confirmed by the DTG and thermogravimetric analysis.					
33676868	5	11	theme	single	619:624	arg1	peak					626:629	a single peak	617:629	a single peak corresponding to a molecular mass of ∼57 kDa	617:674	High performance size exclusion chromatographic analysis of SCTx displayed a single peak corresponding to a molecular mass of ∼57 kDa.					
33676868	1	12	theme	major	111:115	arg1	hemicellulose					117:129	the major hemicellulose	107:129	the major hemicellulose present in sugarcane stem secondary cell walls	107:176	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	1	12	theme	major	111:115	arg1	Xylan					98:102	Xylan	98:102	Xylan	98:102	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	13	13	theme	commercial	1865:1874	arg1	substrate					1876:1884	an alternative commercial substrate	1850:1884	an alternative commercial substrate	1850:1884	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	13	13	theme	commercial	1865:1874	arg1	xylan					1820:1824	The extracted xylan	1806:1824	The extracted xylan from SCT	1806:1833	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	2	14	theme	substitutions	238:250	arg1	degree					228:233	a high degree	221:233	a high degree	221:233	Xylan is composed of xylose backbone with a high degree of substitutions, which affects its properties.					
33676868	1	15	theme	present	131:137	arg1	hemicellulose					117:129	the major hemicellulose	107:129	the major hemicellulose present in sugarcane stem secondary cell walls	107:176	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	1	15	theme	present	131:137	arg1	Xylan					98:102	Xylan	98:102	Xylan	98:102	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	12	16	theme	xylo-oligosaccharides	1656:1676	arg1	series					1639:1644	a series	1637:1644	a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme	1637:1803	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	4	17	theme	xylan	400:404	arg1	analysis					388:395	Compositional analysis	374:395	Compositional analysis of xylan extracted from SCT (SCTx)	374:430	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	1	18	theme	sugarcane	142:150	arg1	walls					172:176	sugarcane stem secondary cell walls	142:176	sugarcane stem secondary cell walls	142:176	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	12	19	theme	SCTx	1622:1625	arg1	analyses					1590:1597	The thin layer chromatography and electrospray ionization mass spectroscopy analyses	1514:1597	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx	1514:1625	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	11	20	theme	1394 ± 51 U/mg	1436:1449	arg1	activity					1424:1431	a specific activity	1413:1431	a specific activity of 1394 ± 51 U/mg	1413:1449	The recombinant xylanase (CtXyn11A) from Clostridium thermocellum displayed a specific activity of 1394 ± 51 U/mg with SCTx, which was higher than those with commercial xylans.					
33676868	0	21	theme	potential	66:74	arg1	substrate					87:95	a potential commercial substrate	64:95	a potential commercial substrate	64:95	Extraction and characterization of xylan from sugarcane tops as a potential commercial substrate.					
33676868	1	22	theme	secondary	157:165	arg1	walls					172:176	sugarcane stem secondary cell walls	142:176	sugarcane stem secondary cell walls	142:176	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	4	23	theme	%	460:460	arg1	presence					446:453	the presence	442:453	the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose	442:539	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	5	24	theme	∼57 kDa	668:674	arg1	mass					660:663	a molecular mass	648:663	a molecular mass of ∼57 kDa	648:674	High performance size exclusion chromatographic analysis of SCTx displayed a single peak corresponding to a molecular mass of ∼57 kDa.					
33676868	0	25	from	sugarcane	46:54	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of xylan from sugarcane tops as a potential commercial substrate.					
33676868	0	25	from	sugarcane	46:54	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of xylan from sugarcane tops as a potential commercial substrate.					
33676868	13	26	used	used	1842:1845	arg2	substrate					1876:1884	an alternative commercial substrate	1850:1884	an alternative commercial substrate	1850:1884	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	13	26	used	used	1842:1845	arg2	xylan					1820:1824	The extracted xylan	1806:1824	The extracted xylan from SCT	1806:1833	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	12	27	theme	CtXyn11A	1602:1609	arg1	SCTx					1622:1625	CtXyn11A hydrolysed SCTx	1602:1625	CtXyn11A hydrolysed SCTx	1602:1625	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	11	28	from	thermocellum	1390:1401	arg1	CtXyn11A					1363:1370	CtXyn11A	1363:1370	CtXyn11A	1363:1370	The recombinant xylanase (CtXyn11A) from Clostridium thermocellum displayed a specific activity of 1394 ± 51 U/mg with SCTx, which was higher than those with commercial xylans.					
33676868	11	28	from	thermocellum	1390:1401	arg1	xylanase					1353:1360	The recombinant xylanase	1337:1360	The recombinant xylanase (CtXyn11A) from Clostridium thermocellum	1337:1401	The recombinant xylanase (CtXyn11A) from Clostridium thermocellum displayed a specific activity of 1394 ± 51 U/mg with SCTx, which was higher than those with commercial xylans.					
33676868	12	29	theme	electrospray	1548:1559	arg1	spectroscopy					1577:1588	electrospray ionization mass spectroscopy	1548:1588	electrospray ionization mass spectroscopy	1548:1588	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	4	30	theme	%	486:486	arg1	presence					446:453	the presence	442:453	the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose	442:539	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	12	31	theme	mass	1572:1575	arg1	spectroscopy					1577:1588	electrospray ionization mass spectroscopy	1548:1588	electrospray ionization mass spectroscopy	1548:1588	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	4	32	theme	d-glucuronic	491:502	arg1	residues					509:516	d-glucuronic acid residues	491:516	d-glucuronic acid residues	491:516	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	13	33	theme	extracted	1810:1818	arg1	substrate					1876:1884	an alternative commercial substrate	1850:1884	an alternative commercial substrate	1850:1884	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	13	33	theme	extracted	1810:1818	arg1	xylan					1820:1824	The extracted xylan	1806:1824	The extracted xylan from SCT	1806:1833	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	0	34	theme	xylan	35:39	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Extraction and characterization of xylan from sugarcane tops as a potential commercial substrate.					
33676868	0	34	theme	xylan	35:39	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterization of xylan from sugarcane tops as a potential commercial substrate.					
33676868	8	35	theme	side	1027:1030	arg1	chains					1032:1037	the side chains	1023:1037	the side chains of glucuronic acid and arabinose and 275°C	1023:1080	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	12	36	theme	endolytic	1776:1784	arg1	activity					1786:1793	the endolytic activity	1772:1793	the endolytic activity of enzyme	1772:1803	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	4	37	theme	d-xylose	465:472	arg1	residues					474:481	d-xylose residues	465:481	d-xylose residues	465:481	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	12	38	theme	enzyme	1798:1803	arg1	activity					1786:1793	the endolytic activity	1772:1793	the endolytic activity of enzyme	1772:1803	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	6	39	theme	commercial	796:805	arg1	xylan					807:811	commercial xylan	796:811	commercial xylan	796:811	The Fourier transform infrared spectroscopic analysis of SCTx displayed the peaks corresponding to those obtained from commercial xylan.					
33676868	8	40	theme	thermogravimetric	900:916	arg1	DTG					928:930	DTG	928:930	DTG	928:930	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	8	40	theme	thermogravimetric	900:916	arg1	analysis					918:925	Differential thermogravimetric analysis	887:925	Differential thermogravimetric analysis (DTG) of SCTx	887:939	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	11	41	theme	commercial	1495:1504	arg1	xylans					1506:1511	commercial xylans	1495:1511	commercial xylans	1495:1511	The recombinant xylanase (CtXyn11A) from Clostridium thermocellum displayed a specific activity of 1394 ± 51 U/mg with SCTx, which was higher than those with commercial xylans.					
33676868	7	42	theme	surface	868:874	arg1	structure					876:884	the granular and porous surface structure	844:884	the granular and porous surface structure	844:884	FESEM analysis of SCTx showed the granular and porous surface structure.					
33676868	8	43	theme	acid	1053:1056	arg1	chains					1032:1037	the side chains	1023:1037	the side chains of glucuronic acid and arabinose and 275°C	1023:1080	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	8	44	theme	arabinose	1062:1070	arg1	chains					1032:1037	the side chains	1023:1037	the side chains of glucuronic acid and arabinose and 275°C	1023:1080	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	6	45	dep	transform	689:697	arg1	infrared					699:706	infrared	699:706	transform infrared spectroscopic analysis of SCTx	689:737	The Fourier transform infrared spectroscopic analysis of SCTx displayed the peaks corresponding to those obtained from commercial xylan.					
33676868	10	46	theme	SCTx	1261:1264	arg1	analysis					1249:1256	The CHNS analysis	1240:1256	The CHNS analysis of SCTx	1240:1264	The CHNS analysis of SCTx showed the presence of only carbon and hydrogen supporting its purity.					
33676868	8	47	theme	SCTx	936:939	arg1	DTG					928:930	DTG	928:930	DTG	928:930	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	8	47	theme	SCTx	936:939	arg1	analysis					918:925	Differential thermogravimetric analysis	887:925	Differential thermogravimetric analysis (DTG) of SCTx	887:939	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	4	48	theme	residues	509:516	arg1	%					524:524	10%	522:524	10% of l-arabinose	522:539	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	48	theme	residues	509:516	arg1	%					460:460	74%	458:460	74% of d-xylose residues	458:481	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	48	theme	residues	509:516	arg1	%					486:486	16%	484:486	16% of d-glucuronic acid residues	484:516	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	48	theme	residues	509:516	arg1	l-arabinose					529:539	l-arabinose	529:539	l-arabinose	529:539	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	48	theme	residues	509:516	arg1	residues					474:481	d-xylose residues	465:481	d-xylose residues	465:481	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	48	theme	residues	509:516	arg1	residues					509:516	d-glucuronic acid residues	491:516	d-glucuronic acid residues	491:516	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	13	49	theme	oligo-saccharide	1894:1909	arg1	production					1911:1920	oligo-saccharide production	1894:1920	oligo-saccharide production	1894:1920	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	5	50	theme	SCTx	602:605	arg1	analysis					590:597	High performance size exclusion chromatographic analysis	542:597	High performance size exclusion chromatographic analysis of SCTx	542:605	High performance size exclusion chromatographic analysis of SCTx displayed a single peak corresponding to a molecular mass of ∼57 kDa.					
33676868	10	51	theme	carbon	1294:1299	arg1	presence					1277:1284	the presence	1273:1284	the presence of only carbon and hydrogen	1273:1312	The CHNS analysis of SCTx showed the presence of only carbon and hydrogen supporting its purity.					
33676868	7	52	theme	SCTx	832:835	arg1	analysis					820:827	FESEM analysis	814:827	FESEM analysis of SCTx	814:835	FESEM analysis of SCTx showed the granular and porous surface structure.					
33676868	4	53	theme	Compositional	374:386	arg1	analysis					388:395	Compositional analysis	374:395	Compositional analysis of xylan extracted from SCT (SCTx)	374:430	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	5	54	theme	performance	547:557	arg1	analysis					590:597	High performance size exclusion chromatographic analysis	542:597	High performance size exclusion chromatographic analysis of SCTx	542:605	High performance size exclusion chromatographic analysis of SCTx displayed a single peak corresponding to a molecular mass of ∼57 kDa.					
33676868	1	55	theme	cell	167:170	arg1	walls					172:176	sugarcane stem secondary cell walls	142:176	sugarcane stem secondary cell walls	142:176	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	8	56	theme	degradation	963:973	arg1	Td					989:990	Td	989:990	Td	989:990	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	8	56	theme	degradation	963:973	arg1	temperatures					975:986	two thermal degradation temperatures	951:986	two thermal degradation temperatures (Td) of 228°C	951:1000	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	5	57	theme	exclusion	564:572	arg1	analysis					590:597	High performance size exclusion chromatographic analysis	542:597	High performance size exclusion chromatographic analysis of SCTx	542:605	High performance size exclusion chromatographic analysis of SCTx displayed a single peak corresponding to a molecular mass of ∼57 kDa.					
33676868	6	58	theme	SCTx	734:737	arg1	analysis					722:729	spectroscopic analysis	708:729	spectroscopic analysis of SCTx	708:737	The Fourier transform infrared spectroscopic analysis of SCTx displayed the peaks corresponding to those obtained from commercial xylan.					
33676868	8	59	theme	xylan	1103:1107	arg1	breakdown					1090:1098	breakdown	1090:1098	breakdown of xylan back bone	1090:1117	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	12	60	theme	thin	1518:1521	arg1	chromatography					1529:1542	thin layer chromatography	1518:1542	thin layer chromatography	1518:1542	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	9	61	theme	arabinose	1136:1144	arg1	presence					1124:1131	The presence	1120:1131	The presence of arabinose and glucuronic acid as a side chains	1120:1181	The presence of arabinose and glucuronic acid as a side chains was confirmed by the DTG and thermogravimetric analysis.					
33676868	12	62	theme	chromatography	1529:1542	arg1	analyses					1590:1597	The thin layer chromatography and electrospray ionization mass spectroscopy analyses	1514:1597	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx	1514:1625	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	5	63	theme	molecular	650:658	arg1	mass					660:663	a molecular mass	648:663	a molecular mass of ∼57 kDa	648:674	High performance size exclusion chromatographic analysis of SCTx displayed a single peak corresponding to a molecular mass of ∼57 kDa.					
33676868	9	64	theme	glucuronic	1150:1159	arg1	acid					1161:1164	glucuronic acid	1150:1164	glucuronic acid	1150:1164	The presence of arabinose and glucuronic acid as a side chains was confirmed by the DTG and thermogravimetric analysis.					
33676868	2	65	theme	high	223:226	arg1	degree					228:233	a high degree	221:233	a high degree	221:233	Xylan is composed of xylose backbone with a high degree of substitutions, which affects its properties.					
33676868	3	66	theme	present	290:296	arg1	study					298:302	the present study	286:302	the present study	286:302	In the present study, the xylan from sugarcane tops (SCT) was extracted and characterized.					
33676868	13	67	theme	alternative	1853:1863	arg1	substrate					1876:1884	an alternative commercial substrate	1850:1884	an alternative commercial substrate	1850:1884	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	13	67	theme	alternative	1853:1863	arg1	xylan					1820:1824	The extracted xylan	1806:1824	The extracted xylan from SCT	1806:1833	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	10	68	theme	hydrogen	1305:1312	arg1	presence					1277:1284	the presence	1273:1284	the presence of only carbon and hydrogen	1273:1312	The CHNS analysis of SCTx showed the presence of only carbon and hydrogen supporting its purity.					
33676868	9	69	theme	side	1171:1174	arg1	chains					1176:1181	a side chains	1169:1181	a side chains	1169:1181	The presence of arabinose and glucuronic acid as a side chains was confirmed by the DTG and thermogravimetric analysis.					
33676868	11	70	theme	Clostridium	1378:1388	arg1	thermocellum					1390:1401	Clostridium thermocellum	1378:1401	Clostridium thermocellum	1378:1401	The recombinant xylanase (CtXyn11A) from Clostridium thermocellum displayed a specific activity of 1394 ± 51 U/mg with SCTx, which was higher than those with commercial xylans.					
33676868	12	71	theme	linear	1649:1654	arg1	xylo-oligosaccharides					1656:1676	linear xylo-oligosaccharides	1649:1676	linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme	1649:1803	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	0	72	theme	commercial	76:85	arg1	substrate					87:95	a potential commercial substrate	64:95	a potential commercial substrate	64:95	Extraction and characterization of xylan from sugarcane tops as a potential commercial substrate.					
33676868	1	73	from	walls	172:176	arg1	present					131:137	present	131:137	present	131:137	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	1	74	theme	stem	152:155	arg1	walls					172:176	sugarcane stem secondary cell walls	142:176	sugarcane stem secondary cell walls	142:176	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	12	75	theme	hydrolysed	1611:1620	arg1	SCTx					1622:1625	CtXyn11A hydrolysed SCTx	1602:1625	CtXyn11A hydrolysed SCTx	1602:1625	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	11	76	theme	specific	1415:1422	arg1	activity					1424:1431	a specific activity	1413:1431	a specific activity of 1394 ± 51 U/mg	1413:1449	The recombinant xylanase (CtXyn11A) from Clostridium thermocellum displayed a specific activity of 1394 ± 51 U/mg with SCTx, which was higher than those with commercial xylans.					
33676868	9	77	theme	DTG	1204:1206	arg1	analysis					1230:1237	the DTG and thermogravimetric analysis	1200:1237	analysis	1230:1237	The presence of arabinose and glucuronic acid as a side chains was confirmed by the DTG and thermogravimetric analysis.					
33676868	8	78	theme	228°C	996:1000	arg1	Td					989:990	Td	989:990	Td	989:990	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	8	78	theme	228°C	996:1000	arg1	temperatures					975:986	two thermal degradation temperatures	951:986	two thermal degradation temperatures (Td) of 228°C	951:1000	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	11	79	theme	recombinant	1341:1351	arg1	CtXyn11A					1363:1370	CtXyn11A	1363:1370	CtXyn11A	1363:1370	The recombinant xylanase (CtXyn11A) from Clostridium thermocellum displayed a specific activity of 1394 ± 51 U/mg with SCTx, which was higher than those with commercial xylans.					
33676868	11	79	theme	recombinant	1341:1351	arg1	xylanase					1353:1360	The recombinant xylanase	1337:1360	The recombinant xylanase (CtXyn11A) from Clostridium thermocellum	1337:1401	The recombinant xylanase (CtXyn11A) from Clostridium thermocellum displayed a specific activity of 1394 ± 51 U/mg with SCTx, which was higher than those with commercial xylans.					
33676868	9	80	theme	thermogravimetric	1212:1228	arg1	analysis					1230:1237	the DTG and thermogravimetric analysis	1200:1237	analysis	1230:1237	The presence of arabinose and glucuronic acid as a side chains was confirmed by the DTG and thermogravimetric analysis.					
33676868	5	81	theme	High	542:545	arg1	analysis					590:597	High performance size exclusion chromatographic analysis	542:597	High performance size exclusion chromatographic analysis of SCTx	542:605	High performance size exclusion chromatographic analysis of SCTx displayed a single peak corresponding to a molecular mass of ∼57 kDa.					
33676868	12	82	theme	spectroscopy	1577:1588	arg1	analyses					1590:1597	The thin layer chromatography and electrospray ionization mass spectroscopy analyses	1514:1597	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx	1514:1625	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	3	83	theme	sugarcane	320:328	arg1	SCT					336:338	SCT	336:338	SCT	336:338	In the present study, the xylan from sugarcane tops (SCT) was extracted and characterized.					
33676868	3	83	theme	sugarcane	320:328	arg1	tops					330:333	sugarcane tops	320:333	sugarcane tops (SCT)	320:339	In the present study, the xylan from sugarcane tops (SCT) was extracted and characterized.					
33676868	1	84	from	present	131:137	arg1	walls					172:176	sugarcane stem secondary cell walls	142:176	sugarcane stem secondary cell walls	142:176	Xylan is the major hemicellulose present in sugarcane stem secondary cell walls.					
33676868	4	85	theme	acid	504:507	arg1	residues					509:516	d-glucuronic acid residues	491:516	d-glucuronic acid residues	491:516	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	12	86	contain	contained	1627:1635	arg1	analyses					1590:1597	The thin layer chromatography and electrospray ionization mass spectroscopy analyses	1514:1597	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx	1514:1625	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	12	86	contain	contained	1627:1635	arg2	series					1639:1644	a series	1637:1644	a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme	1637:1803	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	8	87	theme	chains	1032:1037	arg1	breakdown					1010:1018	breakdown	1010:1018	breakdown of the side chains of glucuronic acid and arabinose and 275°C	1010:1080	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	12	88	theme	ionization	1561:1570	arg1	spectroscopy					1577:1588	electrospray ionization mass spectroscopy	1548:1588	electrospray ionization mass spectroscopy	1548:1588	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
33676868	4	89	theme	residues	474:481	arg1	%					524:524	10%	522:524	10% of l-arabinose	522:539	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	89	theme	residues	474:481	arg1	%					460:460	74%	458:460	74% of d-xylose residues	458:481	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	89	theme	residues	474:481	arg1	%					486:486	16%	484:486	16% of d-glucuronic acid residues	484:516	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	89	theme	residues	474:481	arg1	l-arabinose					529:539	l-arabinose	529:539	l-arabinose	529:539	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	89	theme	residues	474:481	arg1	residues					474:481	d-xylose residues	465:481	d-xylose residues	465:481	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	89	theme	residues	474:481	arg1	residues					509:516	d-glucuronic acid residues	491:516	d-glucuronic acid residues	491:516	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	6	90	theme	spectroscopic	708:720	arg1	analysis					722:729	spectroscopic analysis	708:729	spectroscopic analysis of SCTx	708:737	The Fourier transform infrared spectroscopic analysis of SCTx displayed the peaks corresponding to those obtained from commercial xylan.					
33676868	8	91	theme	glucuronic	1042:1051	arg1	acid					1053:1056	glucuronic acid	1042:1056	glucuronic acid	1042:1056	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	2	92	theme	xylose	200:205	arg1	backbone					207:214	xylose backbone	200:214	xylose backbone with a high degree of substitutions, which affects its properties	200:280	Xylan is composed of xylose backbone with a high degree of substitutions, which affects its properties.					
33676868	10	93	theme	CHNS	1244:1247	arg1	analysis					1249:1256	The CHNS analysis	1240:1256	The CHNS analysis of SCTx	1240:1264	The CHNS analysis of SCTx showed the presence of only carbon and hydrogen supporting its purity.					
33676868	3	94	from	tops	330:333	arg1	xylan					309:313	the xylan	305:313	the xylan from sugarcane tops (SCT)	305:339	In the present study, the xylan from sugarcane tops (SCT) was extracted and characterized.					
33676868	7	95	theme	porous	861:866	arg1	structure					876:884	the granular and porous surface structure	844:884	the granular and porous surface structure	844:884	FESEM analysis of SCTx showed the granular and porous surface structure.					
33676868	13	96	from	SCT	1831:1833	arg1	substrate					1876:1884	an alternative commercial substrate	1850:1884	an alternative commercial substrate	1850:1884	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	13	96	from	SCT	1831:1833	arg1	xylan					1820:1824	The extracted xylan	1806:1824	The extracted xylan from SCT	1806:1833	The extracted xylan from SCT can be used as an alternative commercial substrate and for oligo-saccharide production.					
33676868	8	97	theme	Differential	887:898	arg1	DTG					928:930	DTG	928:930	DTG	928:930	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	8	97	theme	Differential	887:898	arg1	analysis					918:925	Differential thermogravimetric analysis	887:925	Differential thermogravimetric analysis (DTG) of SCTx	887:939	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	4	98	theme	l-arabinose	529:539	arg1	%					524:524	10%	522:524	10% of l-arabinose	522:539	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	98	theme	l-arabinose	529:539	arg1	%					460:460	74%	458:460	74% of d-xylose residues	458:481	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	98	theme	l-arabinose	529:539	arg1	%					486:486	16%	484:486	16% of d-glucuronic acid residues	484:516	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	98	theme	l-arabinose	529:539	arg1	l-arabinose					529:539	l-arabinose	529:539	l-arabinose	529:539	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	98	theme	l-arabinose	529:539	arg1	residues					474:481	d-xylose residues	465:481	d-xylose residues	465:481	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	4	98	theme	l-arabinose	529:539	arg1	residues					509:516	d-glucuronic acid residues	491:516	d-glucuronic acid residues	491:516	Compositional analysis of xylan extracted from SCT (SCTx) displayed the presence of 74% of d-xylose residues, 16% of d-glucuronic acid residues and 10% of l-arabinose.					
33676868	8	99	dep	xylan	1103:1107	arg1	bone					1114:1117	bone	1114:1117	bone	1114:1117	Differential thermogravimetric analysis (DTG) of SCTx displayed two thermal degradation temperatures (Td) of 228°C, due to breakdown of the side chains of glucuronic acid and arabinose and 275°C, due to breakdown of xylan back bone.					
33676868	7	100	theme	granular	848:855	arg1	structure					876:884	the granular and porous surface structure	844:884	the granular and porous surface structure	844:884	FESEM analysis of SCTx showed the granular and porous surface structure.					
33676868	12	101	theme	substituted	1727:1737	arg1	xylo-oligosaccharides					1739:1759	no substituted xylo-oligosaccharides	1724:1759	no substituted xylo-oligosaccharides because of the endolytic activity of enzyme	1724:1803	The thin layer chromatography and electrospray ionization mass spectroscopy analyses of CtXyn11A hydrolysed SCTx contained a series of linear xylo-oligosaccharides ranging from degree of polymerization 2-6 and no substituted xylo-oligosaccharides because of the endolytic activity of enzyme.					
31926926	2	0	theme	reaction	540:547	arg1	condition					549:557	mild reaction condition	535:557	mild reaction condition in aqueous media	535:574	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	1	1	theme	liquid	368:373	arg1	polymerization					287:300	polymerization	287:300	polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid	287:373	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	7	2	theme	condensation	1149:1160	arg1	reactions					1162:1170	similar condensation reactions	1141:1170	similar condensation reactions	1141:1170	Notably, the developed catalyst was versatile and could promote similar condensation reactions to furnish more complex chemicals such as Xanthane.					
31926926	1	3	theme	triple	166:171	arg1	composite					173:181	A novel metal -free triple composite	146:181	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid	146:259	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	0	4	theme	free	84:87	arg1	catalyst					107:114	Metal free and biocompatible catalyst	78:114	Metal free and biocompatible catalyst for chemical transformations	78:143	Functionalized chitosan polymerized with cyclodextrin decorated ionic liquid: Metal free and biocompatible catalyst for chemical transformations.					
31926926	7	5	theme	similar	1141:1147	arg1	reactions					1162:1170	similar condensation reactions	1141:1170	similar condensation reactions	1141:1170	Notably, the developed catalyst was versatile and could promote similar condensation reactions to furnish more complex chemicals such as Xanthane.					
31926926	2	6	theme	resulting	380:388	arg1	compound					390:397	The resulting compound	376:397	The resulting compound	376:397	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	2	6	theme	resulting	380:388	arg1	catalyst					472:479	a heterogeneous catalyst	456:479	a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media	456:574	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	2	6	theme	resulting	380:388	arg1	CS-CD-IL					400:407	CS-CD-IL	400:407	CS-CD-IL	400:407	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	3	7	from	reaction	681:688	arg1	media					701:705	aqueous media	693:705	aqueous media	693:705	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	0	8	theme	biocompatible	93:105	arg1	catalyst					107:114	Metal free and biocompatible catalyst	78:114	Metal free and biocompatible catalyst for chemical transformations	78:143	Functionalized chitosan polymerized with cyclodextrin decorated ionic liquid: Metal free and biocompatible catalyst for chemical transformations.					
31926926	3	9	theme	catalytic	777:785	arg1	sites					787:791	the catalytic sites	773:791	the catalytic sites	773:791	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	6	10	theme	high	1017:1020	arg1	recyclability					1022:1034	high recyclability	1017:1034	high recyclability of the catalyst up to six reaction runs	1017:1074	The results confirmed high recyclability of the catalyst up to six reaction runs.					
31926926	6	11	theme	catalyst	1043:1050	arg1	up					1052:1053	the catalyst up	1039:1053	the catalyst up	1039:1053	The results confirmed high recyclability of the catalyst up to six reaction runs.					
31926926	0	12	theme	chemical	120:127	arg1	transformations					129:143	chemical transformations	120:143	chemical transformations	120:143	Functionalized chitosan polymerized with cyclodextrin decorated ionic liquid: Metal free and biocompatible catalyst for chemical transformations.					
31926926	3	13	theme	sites	787:791	arg1	vicinity					761:768	the vicinity	757:768	the vicinity of the catalytic sites	757:791	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	2	14	theme	aqueous	562:568	arg1	media					570:574	aqueous media	562:574	aqueous media	562:574	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	5	15	theme	catalyst	962:969	arg1	recyclability					941:953	the recyclability	937:953	the reaction variables as well as the recyclability of the catalyst	903:969	The effects of the reaction variables as well as the recyclability of the catalyst were also investigated.					
31926926	5	15	theme	catalyst	962:969	arg1	variables					916:924	the reaction variables	903:924	the reaction variables as well as the recyclability of the catalyst	903:969	The effects of the reaction variables as well as the recyclability of the catalyst were also investigated.					
31926926	2	16	theme	mild	535:538	arg1	condition					549:557	mild reaction condition	535:557	mild reaction condition in aqueous media	535:574	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	3	17	theme	cyclodextrin	614:625	arg1	presence					602:609	the presence	598:609	the presence of cyclodextrin in the structure of the catalyst	598:658	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	3	18	attach	presence	602:609	arg2	cyclodextrin					614:625	cyclodextrin	614:625	cyclodextrin	614:625	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	3	18	attach	presence	602:609	arg1	structure					634:642	the structure	630:642	the structure of the catalyst	630:658	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	0	19	theme	Functionalized	0:13	arg1	chitosan					15:22	Functionalized chitosan	0:22	Functionalized chitosan polymerized with cyclodextrin	0:52	Functionalized chitosan polymerized with cyclodextrin decorated ionic liquid: Metal free and biocompatible catalyst for chemical transformations.					
31926926	5	20	theme	reaction	907:914	arg1	variables					916:924	the reaction variables	903:924	the reaction variables as well as the recyclability of the catalyst	903:969	The effects of the reaction variables as well as the recyclability of the catalyst were also investigated.					
31926926	7	21	theme	developed	1090:1098	arg1	versatile					1113:1121	versatile	1113:1121	versatile	1113:1121	Notably, the developed catalyst was versatile and could promote similar condensation reactions to furnish more complex chemicals such as Xanthane.					
31926926	7	21	theme	developed	1090:1098	arg1	catalyst					1100:1107	the developed catalyst	1086:1107	the developed catalyst	1086:1107	Notably, the developed catalyst was versatile and could promote similar condensation reactions to furnish more complex chemicals such as Xanthane.					
31926926	0	22	theme	polymerized	24:34	arg1	chitosan					15:22	Functionalized chitosan	0:22	Functionalized chitosan polymerized with cyclodextrin	0:52	Functionalized chitosan polymerized with cyclodextrin decorated ionic liquid: Metal free and biocompatible catalyst for chemical transformations.					
31926926	1	23	theme	vinyl	305:309	arg1	chitosan					326:333	functionalized chitosan	311:333	functionalized chitosan	311:333	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	3	24	theme	aqueous	693:699	arg1	media					701:705	aqueous media	693:705	aqueous media	693:705	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	5	25	theme	variables	916:924	arg1	effects					892:898	The effects	888:898	The effects of the reaction variables as well as the recyclability of the catalyst	888:969	The effects of the reaction variables as well as the recyclability of the catalyst were also investigated.					
31926926	3	26	from	substrate	744:752	arg1	vicinity					761:768	the vicinity	757:768	the vicinity of the catalytic sites	757:791	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	1	27	theme	functionalized	311:324	arg1	chitosan					326:333	functionalized chitosan	311:333	functionalized chitosan	311:333	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	1	28	theme	chitosan	219:226	arg1	chemistry					206:214	the chemistry	202:214	the chemistry of chitosan, β-cyclodextrin and ionic liquid	202:259	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	7	29	theme	complex	1188:1194	arg1	chemicals					1196:1204	more complex chemicals	1183:1204	more complex chemicals such as Xanthane	1183:1221	Notably, the developed catalyst was versatile and could promote similar condensation reactions to furnish more complex chemicals such as Xanthane.					
31926926	7	29	theme	complex	1188:1194	arg1	Xanthane					1214:1221	Xanthane	1214:1221	Xanthane	1214:1221	Notably, the developed catalyst was versatile and could promote similar condensation reactions to furnish more complex chemicals such as Xanthane.					
31926926	1	30	theme	chitosan	326:333	arg1	polymerization					287:300	polymerization	287:300	polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid	287:373	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	6	31	theme	reaction	1062:1069	arg1	runs					1071:1074	six reaction runs	1058:1074	six reaction runs	1058:1074	The results confirmed high recyclability of the catalyst up to six reaction runs.					
31926926	2	32	theme	condensation	507:518	arg1	reaction					520:527	Knoevenagel condensation reaction	495:527	Knoevenagel condensation reaction	495:527	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	2	33	from	condition	549:557	arg1	media					570:574	aqueous media	562:574	aqueous media	562:574	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	0	34	with	polymerized	24:34	arg1	cyclodextrin					41:52	cyclodextrin	41:52	cyclodextrin	41:52	Functionalized chitosan polymerized with cyclodextrin decorated ionic liquid: Metal free and biocompatible catalyst for chemical transformations.					
31926926	2	35	theme	Knoevenagel	495:505	arg1	reaction					520:527	Knoevenagel condensation reaction	495:527	Knoevenagel condensation reaction	495:527	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	1	36	from	chemistry	206:214	arg1	benefits					188:195	benefits	188:195	benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid	188:259	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	5	37	theme	recyclability	941:953	arg1	effects					892:898	The effects	888:898	The effects of the reaction variables as well as the recyclability of the catalyst	888:969	The effects of the reaction variables as well as the recyclability of the catalyst were also investigated.					
31926926	3	38	from	presence	602:609	arg1	structure					634:642	the structure	630:642	the structure of the catalyst	630:658	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	3	39	theme	hydrophobic	732:742	arg1	substrate					744:752	the hydrophobic substrate	728:752	the hydrophobic substrate in the vicinity of the catalytic sites	728:791	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	4	40	dep	Chitosan	794:801	arg1	liquid					813:818	liquid	813:818	liquid	813:818	Chitosan and ionic liquid, on the other hand, could activate the substrates via two pathways.					
31926926	1	41	theme	β-cyclodextrin	229:242	arg1	chemistry					206:214	the chemistry	202:214	the chemistry of chitosan, β-cyclodextrin and ionic liquid	202:259	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	4	42	theme	other	828:832	arg1	hand					834:837	the other hand	824:837	the other hand	824:837	Chitosan and ionic liquid, on the other hand, could activate the substrates via two pathways.					
31926926	1	43	theme	novel	148:152	arg1	composite					173:181	A novel metal -free triple composite	146:181	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid	146:259	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	0	44	theme	Metal	78:82	arg1	catalyst					107:114	Metal free and biocompatible catalyst	78:114	Metal free and biocompatible catalyst for chemical transformations	78:143	Functionalized chitosan polymerized with cyclodextrin decorated ionic liquid: Metal free and biocompatible catalyst for chemical transformations.					
31926926	3	45	theme	catalyst	651:658	arg1	structure					634:642	the structure	630:642	the structure of the catalyst	630:658	It was believed that the presence of cyclodextrin in the structure of the catalyst could facilitate the reaction in aqueous media through transferring the hydrophobic substrate in the vicinity of the catalytic sites.					
31926926	1	46	theme	metal	154:158	arg1	composite					173:181	A novel metal -free triple composite	146:181	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid	146:259	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	6	47	theme	up	1052:1053	arg1	recyclability					1022:1034	high recyclability	1017:1034	high recyclability of the catalyst up to six reaction runs	1017:1074	The results confirmed high recyclability of the catalyst up to six reaction runs.					
31926926	2	48	theme	heterogeneous	458:470	arg1	compound					390:397	The resulting compound	376:397	The resulting compound	376:397	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	2	48	theme	heterogeneous	458:470	arg1	catalyst					472:479	a heterogeneous catalyst	456:479	a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media	456:574	The resulting compound, CS-CD-IL, was characterized and successfully applied as a heterogeneous catalyst for promoting Knoevenagel condensation reaction under mild reaction condition in aqueous media.					
31926926	1	49	theme	-free	160:164	arg1	composite					173:181	A novel metal -free triple composite	146:181	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid	146:259	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
31926926	1	50	theme	liquid	254:259	arg1	chemistry					206:214	the chemistry	202:214	the chemistry of chitosan, β-cyclodextrin and ionic liquid	202:259	A novel metal -free triple composite that benefits from the chemistry of chitosan, β-cyclodextrin and ionic liquid has been prepared through polymerization of vinyl functionalized chitosan and cyclodextrin decorated ionic liquid.					
34248947	6	0	theme	negative	1045:1052	arg1	group					1054:1058	the AMA-M2 negative group	1034:1058	the AMA-M2 negative group	1034:1058	For each PBC subgroup, glycan levels of IgG mannose and galactose were decreased in AMA-M2 positive PBC patients compared to the AMA-M2 negative group.					
34248947	13	1	theme	new	1766:1768	arg1	identification					1780:1793	new biomarker identification	1766:1793	new biomarker identification	1766:1793	Altered levels of glycosylation may be related to the occurrence and development of the disease, which could provide a direction for new biomarker identification.					
34248947	10	2	theme	PBC	1385:1387	arg1	subgroups					1389:1397	the above PBC subgroups	1375:1397	the above PBC subgroups	1375:1397	Although the difference in overall sialic acid level was not observed using lectin blot, all results among the above PBC subgroups were consistent with the results of the technique.					
34248947	2	3	theme	glycosylation	273:285	arg1	profile					287:293	the glycosylation profile	269:293	the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology	269:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	5	4	theme	glycan	840:845	arg1	level					847:851	the specific glycan level	827:851	the specific glycan level of serum IgG sialic acid in PBC patients	827:892	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	4	5	theme	PBC	644:646	arg1	subgroups					648:656	PBC subgroups	644:656	PBC subgroups positive for various autoantibodies	644:692	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	2	6	theme	PBC	311:313	arg1	patients					315:322	PBC patients	311:322	PBC patients using high-throughput lectin microarrays technology	311:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	13	7	dep	occurrence	1687:1696	arg1	the					1683:1685	the	1683:1685	the	1683:1685	Altered levels of glycosylation may be related to the occurrence and development of the disease, which could provide a direction for new biomarker identification.					
34248947	12	8	theme	different	1576:1584	arg1	autoantibody					1586:1597	different autoantibody	1576:1597	different autoantibody	1576:1597	PBC patients positive for different autoantibody exhibits distinct glycan profile.					
34248947	5	9	dep	Results	747:753	arg1	revealed					783:790	revealed	783:790	revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased	783:906	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	4	10	theme	control	611:617	arg1	groups					619:624	control groups	611:624	control groups	611:624	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	2	11	theme	IgG	304:306	arg1	profile					287:293	the glycosylation profile	269:293	the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology	269:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	6	12	theme	PBC	918:920	arg1	subgroup					922:929	each PBC subgroup	913:929	each PBC subgroup	913:929	For each PBC subgroup, glycan levels of IgG mannose and galactose were decreased in AMA-M2 positive PBC patients compared to the AMA-M2 negative group.					
34248947	13	13	theme	disease	1721:1727	arg1	development					1702:1712	development	1702:1712	development	1702:1712	Altered levels of glycosylation may be related to the occurrence and development of the disease, which could provide a direction for new biomarker identification.					
34248947	13	13	theme	disease	1721:1727	arg1	occurrence					1687:1696	occurrence	1687:1696	occurrence	1687:1696	Altered levels of glycosylation may be related to the occurrence and development of the disease, which could provide a direction for new biomarker identification.					
34248947	1	14	theme	autoantibody	214:225	arg1	detection					227:235	autoantibody detection	214:235	autoantibody detection	214:235	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	12	15	theme	PBC	1550:1552	arg1	patients					1554:1561	PBC patients	1550:1561	PBC patients positive for different autoantibody	1550:1597	PBC patients positive for different autoantibody exhibits distinct glycan profile.					
34248947	5	16	theme	PBC	881:883	arg1	patients					885:892	PBC patients	881:892	PBC patients	881:892	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	2	17	from	profile	287:293	arg1	patients					315:322	PBC patients	311:322	PBC patients using high-throughput lectin microarrays technology	311:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	12	18	theme	positive	1563:1570	arg1	patients					1554:1561	PBC patients	1550:1561	PBC patients positive for different autoantibody	1550:1597	PBC patients positive for different autoantibody exhibits distinct glycan profile.					
34248947	10	19	from	difference	1281:1290	arg1	level					1315:1319	overall sialic acid level	1295:1319	overall sialic acid level	1295:1319	Although the difference in overall sialic acid level was not observed using lectin blot, all results among the above PBC subgroups were consistent with the results of the technique.					
34248947	11	20	theme	Lectin	1461:1466	arg1	technique					1508:1516	an effective and reliable technique	1482:1516	an effective and reliable technique for analyzing glycan structure	1482:1547	Conclusion Lectin microarray is an effective and reliable technique for analyzing glycan structure.					
34248947	11	20	theme	Lectin	1461:1466	arg1	microarray					1468:1477	Conclusion Lectin microarray	1450:1477	Conclusion Lectin microarray	1450:1477	Conclusion Lectin microarray is an effective and reliable technique for analyzing glycan structure.					
34248947	5	21	from	level	847:851	arg1	patients					885:892	PBC patients	881:892	PBC patients	881:892	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	4	22	theme	positive	658:665	arg1	subgroups					648:656	PBC subgroups	644:656	PBC subgroups positive for various autoantibodies	644:692	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	0	23	theme	Biliary	55:61	arg1	Patients					75:82	Primary Biliary Cholangitis Patients	47:82	Primary Biliary Cholangitis Patients	47:82	Changes of Serum IgG Glycosylation Patterns in Primary Biliary Cholangitis Patients.					
34248947	9	24	theme	ACA-positive	1245:1256	arg1	patients					1258:1265	ACA-positive patients	1245:1265	ACA-positive patients	1245:1265	IgG mannose was decreased in ACA-positive patients.					
34248947	5	25	theme	IgG	862:864	arg1	acid					873:876	serum IgG sialic acid	856:876	serum IgG sialic acid in PBC patients	856:892	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	4	26	theme	various	671:677	arg1	autoantibodies					679:692	various autoantibodies	671:692	various autoantibodies	671:692	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	11	27	theme	effective	1485:1493	arg1	technique					1508:1516	an effective and reliable technique	1482:1516	an effective and reliable technique for analyzing glycan structure	1482:1547	Conclusion Lectin microarray is an effective and reliable technique for analyzing glycan structure.					
34248947	11	27	theme	effective	1485:1493	arg1	microarray					1468:1477	Conclusion Lectin microarray	1450:1477	Conclusion Lectin microarray	1450:1477	Conclusion Lectin microarray is an effective and reliable technique for analyzing glycan structure.					
34248947	5	28	theme	Lectin	755:760	arg1	detection					773:781	Lectin microarray detection	755:781	Lectin microarray detection	755:781	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	1	29	theme	liver	158:162	arg1	disease					164:170	an autoimmune cholestatic liver disease	132:170	an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection	132:235	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	1	29	theme	liver	158:162	arg1	cholangitis					111:121	Objective Primary biliary cholangitis	85:121	Objective Primary biliary cholangitis (PBC)	85:127	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	2	30	from	patients	315:322	arg1	profile					287:293	the glycosylation profile	269:293	the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology	269:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	6	31	theme	IgG	949:951	arg1	mannose					953:959	IgG mannose	949:959	IgG mannose	949:959	For each PBC subgroup, glycan levels of IgG mannose and galactose were decreased in AMA-M2 positive PBC patients compared to the AMA-M2 negative group.					
34248947	3	32	theme	Method	377:382	arg1	microarray					391:400	Method Lectin microarray	377:400	Method Lectin microarray containing 56 lectins	377:422	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	0	33	from	Changes	0:6	arg1	Patients					75:82	Primary Biliary Cholangitis Patients	47:82	Primary Biliary Cholangitis Patients	47:82	Changes of Serum IgG Glycosylation Patterns in Primary Biliary Cholangitis Patients.					
34248947	0	34	theme	Patterns	35:42	arg1	Changes					0:6	Changes	0:6	Changes of Serum IgG Glycosylation Patterns in Primary Biliary Cholangitis Patients.	0:83	Changes of Serum IgG Glycosylation Patterns in Primary Biliary Cholangitis Patients.					
34248947	5	35	from	patients	885:892	arg1	level					847:851	the specific glycan level	827:851	the specific glycan level of serum IgG sialic acid in PBC patients	827:892	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	13	36	theme	biomarker	1770:1778	arg1	identification					1780:1793	new biomarker identification	1766:1793	new biomarker identification	1766:1793	Altered levels of glycosylation may be related to the occurrence and development of the disease, which could provide a direction for new biomarker identification.					
34248947	7	37	theme	positive	1136:1143	arg1	patients					1145:1152	anti-sp100 positive patients	1125:1152	anti-sp100 positive patients	1125:1152	IgG N-Acetylgalactosamine (GalNAc) and fucose were decreased in anti-sp100 positive patients.					
34248947	1	38	theme	Primary	95:101	arg1	disease					164:170	an autoimmune cholestatic liver disease	132:170	an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection	132:235	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	1	38	theme	Primary	95:101	arg1	PBC					124:126	PBC	124:126	PBC	124:126	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	1	38	theme	Primary	95:101	arg1	cholangitis					111:121	Objective Primary biliary cholangitis	85:121	Objective Primary biliary cholangitis (PBC)	85:127	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	4	39	theme	blot	731:734	arg1	technique					736:744	lectin blot technique	724:744	lectin blot technique	724:744	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	3	40	theme	IgG	479:481	arg1	glycosylation					483:495	serum IgG glycosylation	473:495	serum IgG glycosylation	473:495	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	0	41	theme	Serum	11:15	arg1	Patterns					35:42	Serum IgG Glycosylation Patterns	11:42	Serum IgG Glycosylation Patterns	11:42	Changes of Serum IgG Glycosylation Patterns in Primary Biliary Cholangitis Patients.					
34248947	6	42	theme	positive	1000:1007	arg1	patients					1013:1020	AMA-M2 positive PBC patients	993:1020	AMA-M2 positive PBC patients	993:1020	For each PBC subgroup, glycan levels of IgG mannose and galactose were decreased in AMA-M2 positive PBC patients compared to the AMA-M2 negative group.					
34248947	13	43	theme	glycosylation	1651:1663	arg1	levels					1641:1646	Altered levels	1633:1646	Altered levels of glycosylation	1633:1663	Altered levels of glycosylation may be related to the occurrence and development of the disease, which could provide a direction for new biomarker identification.					
34248947	0	44	theme	Glycosylation	21:33	arg1	Patterns					35:42	Serum IgG Glycosylation Patterns	11:42	Serum IgG Glycosylation Patterns	11:42	Changes of Serum IgG Glycosylation Patterns in Primary Biliary Cholangitis Patients.					
34248947	3	45	theme	PBC	503:505	arg1	patients					507:514	99 PBC patients	500:514	99 PBC patients	500:514	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	10	46	theme	sialic	1303:1308	arg1	level					1315:1319	overall sialic acid level	1295:1319	overall sialic acid level	1295:1319	Although the difference in overall sialic acid level was not observed using lectin blot, all results among the above PBC subgroups were consistent with the results of the technique.					
34248947	2	47	theme	high-throughput	330:344	arg1	technology					365:374	high-throughput lectin microarrays technology	330:374	high-throughput lectin microarrays technology	330:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	1	48	theme	autoimmune	135:144	arg1	disease					164:170	an autoimmune cholestatic liver disease	132:170	an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection	132:235	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	1	48	theme	autoimmune	135:144	arg1	cholangitis					111:121	Objective Primary biliary cholangitis	85:121	Objective Primary biliary cholangitis (PBC)	85:127	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	4	49	theme	Significant	575:585	arg1	differences					587:597	Significant differences	575:597	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies	575:692	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	3	50	theme	disease	520:526	arg1	DCs					538:540	DCs	538:540	DCs	538:540	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	3	50	theme	disease	520:526	arg1	controls					528:535	70 disease controls	517:535	70 disease controls (DCs)	517:541	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	3	51	from	expression	459:468	arg1	controls					528:535	70 disease controls	517:535	70 disease controls (DCs)	517:541	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	3	51	from	expression	459:468	arg1	patients					507:514	99 PBC patients	500:514	99 PBC patients	500:514	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	3	51	from	expression	459:468	arg1	DCs					538:540	DCs	538:540	DCs	538:540	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	3	51	from	expression	459:468	arg1	HCs					569:571	HCs	569:571	HCs	569:571	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	3	51	from	expression	459:468	arg1	controls					559:566	38 healthy controls	548:566	38 healthy controls (HCs)	548:572	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	6	52	theme	AMA-M2	1038:1043	arg1	group					1054:1058	the AMA-M2 negative group	1034:1058	the AMA-M2 negative group	1034:1058	For each PBC subgroup, glycan levels of IgG mannose and galactose were decreased in AMA-M2 positive PBC patients compared to the AMA-M2 negative group.					
34248947	7	53	theme	IgG	1061:1063	arg1	N-Acetylgalactosamine					1065:1085	IgG N-Acetylgalactosamine	1061:1085	IgG N-Acetylgalactosamine (GalNAc)	1061:1094	IgG N-Acetylgalactosamine (GalNAc) and fucose were decreased in anti-sp100 positive patients.					
34248947	7	53	theme	IgG	1061:1063	arg1	GalNAc					1088:1093	GalNAc	1088:1093	GalNAc	1088:1093	IgG N-Acetylgalactosamine (GalNAc) and fucose were decreased in anti-sp100 positive patients.					
34248947	2	54	theme	serum	298:302	arg1	IgG					304:306	serum IgG	298:306	serum IgG in PBC patients using high-throughput lectin microarrays technology	298:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	5	55	theme	sialic	866:871	arg1	acid					873:876	serum IgG sialic acid	856:876	serum IgG sialic acid in PBC patients	856:892	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	2	56	gly	glycosylation	273:285	arg1	IgG					304:306	serum IgG	298:306	serum IgG in PBC patients using high-throughput lectin microarrays technology	298:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	2	57	theme	microarrays	353:363	arg1	technology					365:374	high-throughput lectin microarrays technology	330:374	high-throughput lectin microarrays technology	330:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	11	58	theme	reliable	1499:1506	arg1	technique					1508:1516	an effective and reliable technique	1482:1516	an effective and reliable technique for analyzing glycan structure	1482:1547	Conclusion Lectin microarray is an effective and reliable technique for analyzing glycan structure.					
34248947	11	58	theme	reliable	1499:1506	arg1	microarray					1468:1477	Conclusion Lectin microarray	1450:1477	Conclusion Lectin microarray	1450:1477	Conclusion Lectin microarray is an effective and reliable technique for analyzing glycan structure.					
34248947	5	59	theme	specific	831:838	arg1	level					847:851	the specific glycan level	827:851	the specific glycan level of serum IgG sialic acid in PBC patients	827:892	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	3	60	contain	containing	402:411	arg1	microarray					391:400	Method Lectin microarray	377:400	Method Lectin microarray containing 56 lectins	377:422	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	3	60	contain	containing	402:411	arg2	lectins					416:422	56 lectins	413:422	56 lectins	413:422	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	8	61	theme	positive	1197:1204	arg1	patients					1206:1213	anti-gp210 positive patients	1186:1213	anti-gp210 positive patients	1186:1213	IgG galactose was increased in anti-gp210 positive patients.					
34248947	10	62	theme	above	1379:1383	arg1	subgroups					1389:1397	the above PBC subgroups	1375:1397	the above PBC subgroups	1375:1397	Although the difference in overall sialic acid level was not observed using lectin blot, all results among the above PBC subgroups were consistent with the results of the technique.					
34248947	3	63	theme	healthy	551:557	arg1	HCs					569:571	HCs	569:571	HCs	569:571	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	3	63	theme	healthy	551:557	arg1	controls					559:566	38 healthy controls	548:566	38 healthy controls (HCs)	548:572	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	6	64	theme	glycan	932:937	arg1	levels					939:944	glycan levels	932:944	glycan levels of IgG mannose and galactose	932:973	For each PBC subgroup, glycan levels of IgG mannose and galactose were decreased in AMA-M2 positive PBC patients compared to the AMA-M2 negative group.					
34248947	11	65	theme	glycan	1532:1537	arg1	structure					1539:1547	glycan structure	1532:1547	glycan structure	1532:1547	Conclusion Lectin microarray is an effective and reliable technique for analyzing glycan structure.					
34248947	4	66	from	groups	619:624	arg1	differences					587:597	Significant differences	575:597	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies	575:692	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	4	66	from	groups	619:624	arg1	PBC					602:604	PBC	602:604	PBC from control groups as well as across PBC subgroups positive for various autoantibodies	602:692	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	5	67	theme	HC	816:817	arg1	groups					819:824	HC groups	816:824	HC groups	816:824	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	8	68	theme	IgG	1155:1157	arg1	galactose					1159:1167	IgG galactose	1155:1167	IgG galactose	1155:1167	IgG galactose was increased in anti-gp210 positive patients.					
34248947	12	69	theme	distinct	1608:1615	arg1	profile					1624:1630	distinct glycan profile	1608:1630	distinct glycan profile	1608:1630	PBC patients positive for different autoantibody exhibits distinct glycan profile.					
34248947	5	70	theme	acid	873:876	arg1	level					847:851	the specific glycan level	827:851	the specific glycan level of serum IgG sialic acid in PBC patients	827:892	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	9	71	theme	IgG	1216:1218	arg1	mannose					1220:1226	IgG mannose	1216:1226	IgG mannose	1216:1226	IgG mannose was decreased in ACA-positive patients.					
34248947	11	72	theme	Conclusion	1450:1459	arg1	technique					1508:1516	an effective and reliable technique	1482:1516	an effective and reliable technique for analyzing glycan structure	1482:1547	Conclusion Lectin microarray is an effective and reliable technique for analyzing glycan structure.					
34248947	11	72	theme	Conclusion	1450:1459	arg1	microarray					1468:1477	Conclusion Lectin microarray	1450:1477	Conclusion Lectin microarray	1450:1477	Conclusion Lectin microarray is an effective and reliable technique for analyzing glycan structure.					
34248947	0	73	theme	Cholangitis	63:73	arg1	Patients					75:82	Primary Biliary Cholangitis Patients	47:82	Primary Biliary Cholangitis Patients	47:82	Changes of Serum IgG Glycosylation Patterns in Primary Biliary Cholangitis Patients.					
34248947	5	74	theme	serum	856:860	arg1	acid					873:876	serum IgG sialic acid	856:876	serum IgG sialic acid in PBC patients	856:892	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	6	75	theme	galactose	965:973	arg1	levels					939:944	glycan levels	932:944	glycan levels of IgG mannose and galactose	932:973	For each PBC subgroup, glycan levels of IgG mannose and galactose were decreased in AMA-M2 positive PBC patients compared to the AMA-M2 negative group.					
34248947	4	76	from	differences	587:597	arg1	groups					619:624	control groups	611:624	control groups	611:624	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	4	76	from	differences	587:597	arg1	PBC					602:604	PBC	602:604	PBC from control groups as well as across PBC subgroups positive for various autoantibodies	602:692	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	1	77	theme	cholestatic	146:156	arg1	disease					164:170	an autoimmune cholestatic liver disease	132:170	an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection	132:235	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	1	77	theme	cholestatic	146:156	arg1	cholangitis					111:121	Objective Primary biliary cholangitis	85:121	Objective Primary biliary cholangitis (PBC)	85:127	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	2	78	from	IgG	304:306	arg1	patients					315:322	PBC patients	311:322	PBC patients using high-throughput lectin microarrays technology	311:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	6	79	theme	mannose	953:959	arg1	levels					939:944	glycan levels	932:944	glycan levels of IgG mannose and galactose	932:973	For each PBC subgroup, glycan levels of IgG mannose and galactose were decreased in AMA-M2 positive PBC patients compared to the AMA-M2 negative group.					
34248947	5	80	from	acid	873:876	arg1	patients					885:892	PBC patients	881:892	PBC patients	881:892	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	3	81	theme	Lectin	384:389	arg1	microarray					391:400	Method Lectin microarray	377:400	Method Lectin microarray containing 56 lectins	377:422	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	1	82	theme	Objective	85:93	arg1	disease					164:170	an autoimmune cholestatic liver disease	132:170	an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection	132:235	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	1	82	theme	Objective	85:93	arg1	PBC					124:126	PBC	124:126	PBC	124:126	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	1	82	theme	Objective	85:93	arg1	cholangitis					111:121	Objective Primary biliary cholangitis	85:121	Objective Primary biliary cholangitis (PBC)	85:127	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	10	83	with	consistent	1404:1413	arg1	results					1424:1430	the results	1420:1430	the results of the technique	1420:1447	Although the difference in overall sialic acid level was not observed using lectin blot, all results among the above PBC subgroups were consistent with the results of the technique.					
34248947	10	84	theme	overall	1295:1301	arg1	level					1315:1319	overall sialic acid level	1295:1319	overall sialic acid level	1295:1319	Although the difference in overall sialic acid level was not observed using lectin blot, all results among the above PBC subgroups were consistent with the results of the technique.					
34248947	0	85	theme	IgG	17:19	arg1	Patterns					35:42	Serum IgG Glycosylation Patterns	11:42	Serum IgG Glycosylation Patterns	11:42	Changes of Serum IgG Glycosylation Patterns in Primary Biliary Cholangitis Patients.					
34248947	1	86	theme	biliary	103:109	arg1	disease					164:170	an autoimmune cholestatic liver disease	132:170	an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection	132:235	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	1	86	theme	biliary	103:109	arg1	PBC					124:126	PBC	124:126	PBC	124:126	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	1	86	theme	biliary	103:109	arg1	cholangitis					111:121	Objective Primary biliary cholangitis	85:121	Objective Primary biliary cholangitis (PBC)	85:127	Objective Primary biliary cholangitis (PBC) is an autoimmune cholestatic liver disease whose diagnosis is based significantly on autoantibody detection.					
34248947	12	87	theme	glycan	1617:1622	arg1	profile					1624:1630	distinct glycan profile	1608:1630	distinct glycan profile	1608:1630	PBC patients positive for different autoantibody exhibits distinct glycan profile.					
34248947	6	88	theme	PBC	1009:1011	arg1	patients					1013:1020	AMA-M2 positive PBC patients	993:1020	AMA-M2 positive PBC patients	993:1020	For each PBC subgroup, glycan levels of IgG mannose and galactose were decreased in AMA-M2 positive PBC patients compared to the AMA-M2 negative group.					
34248947	4	89	theme	lectin	724:729	arg1	technique					736:744	lectin blot technique	724:744	lectin blot technique	724:744	Significant differences in PBC from control groups as well as across PBC subgroups positive for various autoantibodies were explored and verified by lectin blot technique.					
34248947	3	90	theme	glycosylation	483:495	arg1	expression					459:468	the expression	455:468	the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs)	455:572	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	5	91	theme	microarray	762:771	arg1	detection					773:781	Lectin microarray detection	755:781	Lectin microarray detection	755:781	Results Lectin microarray detection revealed that compared to DC and HC groups, the specific glycan level of serum IgG sialic acid in PBC patients was increased.					
34248947	10	92	theme	technique	1439:1447	arg1	results					1424:1430	the results	1420:1430	the results of the technique	1420:1447	Although the difference in overall sialic acid level was not observed using lectin blot, all results among the above PBC subgroups were consistent with the results of the technique.					
34248947	3	93	theme	serum	473:477	arg1	glycosylation					483:495	serum IgG glycosylation	473:495	serum IgG glycosylation	473:495	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	0	94	theme	Primary	47:53	arg1	Patients					75:82	Primary Biliary Cholangitis Patients	47:82	Primary Biliary Cholangitis Patients	47:82	Changes of Serum IgG Glycosylation Patterns in Primary Biliary Cholangitis Patients.					
34248947	3	95	used	used	428:431	arg2	microarray					391:400	Method Lectin microarray	377:400	Method Lectin microarray containing 56 lectins	377:422	Method Lectin microarray containing 56 lectins was used to detect and analyze the expression of serum IgG glycosylation in 99 PBC patients, 70 disease controls (DCs), and 38 healthy controls (HCs).					
34248947	10	96	theme	acid	1310:1313	arg1	level					1315:1319	overall sialic acid level	1295:1319	overall sialic acid level	1295:1319	Although the difference in overall sialic acid level was not observed using lectin blot, all results among the above PBC subgroups were consistent with the results of the technique.					
34248947	2	97	theme	lectin	346:351	arg1	technology					365:374	high-throughput lectin microarrays technology	330:374	high-throughput lectin microarrays technology	330:374	This study aims to investigate the glycosylation profile of serum IgG in PBC patients using high-throughput lectin microarrays technology.					
34248947	13	98	theme	Altered	1633:1639	arg1	levels					1641:1646	Altered levels	1633:1646	Altered levels of glycosylation	1633:1663	Altered levels of glycosylation may be related to the occurrence and development of the disease, which could provide a direction for new biomarker identification.					
34248947	10	99	theme	lectin	1344:1349	arg1	blot					1351:1354	lectin blot	1344:1354	lectin blot	1344:1354	Although the difference in overall sialic acid level was not observed using lectin blot, all results among the above PBC subgroups were consistent with the results of the technique.					
32475593	7	0	theme	monosaccharide	1117:1130	arg1	analysis					1144:1151	monosaccharide composition analysis	1117:1151	monosaccharide composition analysis of complex, acidic and basic polysaccharides	1117:1196	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	4	1	theme	loss	704:707	arg1	ratio					709:713	The hydrolysis loss ratio	689:713	The hydrolysis loss ratio for monosaccharide standards	689:742	The hydrolysis loss ratio for monosaccharide standards were also measured.					
32475593	7	2	theme	composition	1132:1142	arg1	analysis					1144:1151	monosaccharide composition analysis	1117:1151	monosaccharide composition analysis of complex, acidic and basic polysaccharides	1117:1196	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	1	3	theme	structural	177:186	arg1	characterization					188:203	structural characterization	177:203	structural characterization of the polysaccharides	177:226	Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides.					
32475593	7	4	from	conclusion	1051:1060	arg1	suitable					1104:1111	suitable	1104:1111	suitable	1104:1111	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	3	5	theme	complex	550:556	arg1	polysaccharides					576:590	complex, basic and acidic polysaccharides	550:590	complex, basic and acidic polysaccharides	550:590	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	5	6	theme	HPLC	954:957	arg1	analysis					959:966	HPLC analysis	954:966	HPLC analysis	954:966	Using this assay, the hydrolysis plus 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of the monosaccharide products could be accomplished in 90 min with the RSD values less than 5 % based on HPLC analysis.					
32475593	3	7	theme	acidic	569:574	arg1	polysaccharides					576:590	complex, basic and acidic polysaccharides	550:590	complex, basic and acidic polysaccharides	550:590	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	2	8	used	used	271:274	arg2	we					268:269	we	268:269	we	268:269	To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min.					
32475593	5	9	dep	values	924:929	arg1	%					943:943	less than 5 %	931:943	the RSD values less than 5 % based on HPLC analysis	916:966	Using this assay, the hydrolysis plus 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of the monosaccharide products could be accomplished in 90 min with the RSD values less than 5 % based on HPLC analysis.					
32475593	5	10	theme	RSD	920:922	arg1	values					924:929	the RSD values	916:929	the RSD values less than 5 % based on HPLC analysis	916:966	Using this assay, the hydrolysis plus 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of the monosaccharide products could be accomplished in 90 min with the RSD values less than 5 % based on HPLC analysis.					
32475593	2	11	theme	reaction	295:302	arg1	instrument					310:319	a polymerase chain reaction (PCR) instrument	276:319	a polymerase chain reaction (PCR) instrument	276:319	To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min.					
32475593	7	12	theme	basic	1176:1180	arg1	polysaccharides					1182:1196	complex, acidic and basic polysaccharides	1156:1196	complex, acidic and basic polysaccharides	1156:1196	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	7	13	theme	instrument-based	1067:1082	arg1	assay					1095:1099	PCR instrument-based hydrolysis assay	1063:1099	PCR instrument-based hydrolysis assay	1063:1099	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	1	14	theme	Monosaccharide	93:106	arg1	step					164:167	the first step	154:167	the first step towards structural characterization of the polysaccharides	154:226	Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides.					
32475593	1	14	theme	Monosaccharide	93:106	arg1	analysis					120:127	Monosaccharide composition analysis	93:127	Monosaccharide composition analysis after acid hydrolysis	93:149	Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides.					
32475593	5	15	theme	PMP	834:836	arg1	labeling					839:846	1-phenyl-3-methyl-5-pyrazolone (PMP) labeling	802:846	1-phenyl-3-methyl-5-pyrazolone (PMP) labeling	802:846	Using this assay, the hydrolysis plus 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of the monosaccharide products could be accomplished in 90 min with the RSD values less than 5 % based on HPLC analysis.					
32475593	5	16	dep	hydrolysis	786:795	arg1	the					782:784	the	782:784	the	782:784	Using this assay, the hydrolysis plus 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of the monosaccharide products could be accomplished in 90 min with the RSD values less than 5 % based on HPLC analysis.					
32475593	3	17	theme	polysaccharides	576:590	arg1	representatives					531:545	representatives	531:545	representatives of complex, basic and acidic polysaccharides	531:590	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	17	theme	polysaccharides	576:590	arg1	chitosan					456:463	chitosan	456:463	chitosan	456:463	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	17	theme	polysaccharides	576:590	arg1	glycol					479:484	propylene glycol	469:484	propylene glycol	469:484	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	17	theme	polysaccharides	576:590	arg1	Fucoidan					446:453	Fucoidan	446:453	Fucoidan	446:453	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	1	18	theme	polysaccharides	212:226	arg1	characterization					188:203	structural characterization	177:203	structural characterization of the polysaccharides	177:226	Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides.					
32475593	7	19	theme	acidic	1165:1170	arg1	polysaccharides					1182:1196	complex, acidic and basic polysaccharides	1156:1196	complex, acidic and basic polysaccharides	1156:1196	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	3	20	theme	basic	559:563	arg1	polysaccharides					576:590	complex, basic and acidic polysaccharides	550:590	complex, basic and acidic polysaccharides	550:590	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	1	21	theme	composition	108:118	arg1	step					164:167	the first step	154:167	the first step towards structural characterization of the polysaccharides	154:226	Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides.					
32475593	1	21	theme	composition	108:118	arg1	analysis					120:127	Monosaccharide composition analysis	93:127	Monosaccharide composition analysis after acid hydrolysis	93:149	Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides.					
32475593	7	22	theme	PCR	1063:1065	arg1	assay					1095:1099	PCR instrument-based hydrolysis assay	1063:1099	PCR instrument-based hydrolysis assay	1063:1099	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	3	23	theme	orthogonal	657:666	arg1	34					672:673	34	672:673	34	672:673	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	23	theme	orthogonal	657:666	arg1	L9					668:669	the orthogonal L9	653:669	the orthogonal L9 (34) experiments	653:686	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	4	24	theme	hydrolysis	693:702	arg1	ratio					709:713	The hydrolysis loss ratio	689:713	The hydrolysis loss ratio for monosaccharide standards	689:742	The hydrolysis loss ratio for monosaccharide standards were also measured.					
32475593	3	25	theme	alginate	486:493	arg1	PSS					511:513	PSS	511:513	PSS	511:513	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	25	theme	alginate	486:493	arg1	sulfate					502:508	alginate sodium sulfate	486:508	alginate sodium sulfate (PSS)	486:514	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	7	26	theme	complex	1156:1162	arg1	polysaccharides					1182:1196	complex, acidic and basic polysaccharides	1156:1196	complex, acidic and basic polysaccharides	1156:1196	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	3	27	theme	L9	668:669	arg1	experiments					676:686	the orthogonal L9 (34) experiments	653:686	the orthogonal L9 (34) experiments	653:686	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	5	28	theme	monosaccharide	855:868	arg1	products					870:877	the monosaccharide products	851:877	the monosaccharide products	851:877	Using this assay, the hydrolysis plus 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of the monosaccharide products could be accomplished in 90 min with the RSD values less than 5 % based on HPLC analysis.					
32475593	7	29	from	suitable	1104:1111	arg1	conclusion					1051:1060	conclusion	1051:1060	conclusion	1051:1060	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	3	30	theme	hydrolytic	608:617	arg1	conditions					619:628	the hydrolytic conditions	604:628	the hydrolytic conditions	604:628	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	31	theme	propylene	469:477	arg1	representatives					531:545	representatives	531:545	representatives of complex, basic and acidic polysaccharides	531:590	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	31	theme	propylene	469:477	arg1	chitosan					456:463	chitosan	456:463	chitosan	456:463	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	31	theme	propylene	469:477	arg1	glycol					479:484	propylene glycol	469:484	propylene glycol	469:484	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	31	theme	propylene	469:477	arg1	Fucoidan					446:453	Fucoidan	446:453	Fucoidan	446:453	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	6	32	theme	coupled	1027:1033	arg1	analysis					1038:1045	HPLC coupled MS analysis	1022:1045	HPLC coupled MS analysis	1022:1045	We further confirmed the reliability of the assay by HPLC coupled MS analysis.					
32475593	1	33	theme	acid	135:138	arg1	hydrolysis					140:149	acid hydrolysis	135:149	acid hydrolysis	135:149	Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides.					
32475593	5	34	theme	1-phenyl-3-methyl-5-pyrazolone	802:831	arg1	labeling					839:846	1-phenyl-3-methyl-5-pyrazolone (PMP) labeling	802:846	1-phenyl-3-methyl-5-pyrazolone (PMP) labeling	802:846	Using this assay, the hydrolysis plus 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of the monosaccharide products could be accomplished in 90 min with the RSD values less than 5 % based on HPLC analysis.					
32475593	2	35	theme	hydrolytic	246:255	arg1	procedure					257:265	the hydrolytic procedure	242:265	the hydrolytic procedure	242:265	To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min.					
32475593	2	36	theme	chain	289:293	arg1	PCR					305:307	PCR	305:307	PCR	305:307	To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min.					
32475593	2	36	theme	chain	289:293	arg1	reaction					295:302	polymerase chain reaction	278:302	a polymerase chain reaction (PCR) instrument	276:319	To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min.					
32475593	3	37	dep	Fucoidan	446:453	arg1	PSS					511:513	PSS	511:513	PSS	511:513	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	37	dep	Fucoidan	446:453	arg1	sulfate					502:508	alginate sodium sulfate	486:508	alginate sodium sulfate (PSS)	486:514	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	38	theme	sodium	495:500	arg1	PSS					511:513	PSS	511:513	PSS	511:513	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	3	38	theme	sodium	495:500	arg1	sulfate					502:508	alginate sodium sulfate	486:508	alginate sodium sulfate (PSS)	486:514	Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (34) experiments.					
32475593	6	39	theme	HPLC	1022:1025	arg1	analysis					1038:1045	HPLC coupled MS analysis	1022:1045	HPLC coupled MS analysis	1022:1045	We further confirmed the reliability of the assay by HPLC coupled MS analysis.					
32475593	2	40	theme	polymerase	278:287	arg1	PCR					305:307	PCR	305:307	PCR	305:307	To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min.					
32475593	2	40	theme	polymerase	278:287	arg1	reaction					295:302	polymerase chain reaction	278:302	a polymerase chain reaction (PCR) instrument	276:319	To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min.					
32475593	4	41	theme	monosaccharide	719:732	arg1	standards					734:742	monosaccharide standards	719:742	monosaccharide standards	719:742	The hydrolysis loss ratio for monosaccharide standards were also measured.					
32475593	7	42	theme	hydrolysis	1084:1093	arg1	assay					1095:1099	PCR instrument-based hydrolysis assay	1063:1099	PCR instrument-based hydrolysis assay	1063:1099	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	0	43	theme	composition	71:81	arg1	analyses					83:90	monosaccharide composition analyses	56:90	monosaccharide composition analyses	56:90	Using a PCR instrument to hydrolyze polysaccharides for monosaccharide composition analyses.					
32475593	5	44	theme	products	870:877	arg1	hydrolysis					786:795	hydrolysis	786:795	hydrolysis	786:795	Using this assay, the hydrolysis plus 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of the monosaccharide products could be accomplished in 90 min with the RSD values less than 5 % based on HPLC analysis.					
32475593	2	45	dep	96	405:406	arg1	to					402:403	to	402:403	to	402:403	To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min.					
32475593	2	46	from	samples	408:414	arg1	products					385:392	monosaccharide products	370:392	monosaccharide products from up to 96 samples	370:414	To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min.					
32475593	1	47	theme	first	158:162	arg1	step					164:167	the first step	154:167	the first step towards structural characterization of the polysaccharides	154:226	Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides.					
32475593	1	47	theme	first	158:162	arg1	analysis					120:127	Monosaccharide composition analysis	93:127	Monosaccharide composition analysis after acid hydrolysis	93:149	Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides.					
32475593	6	48	theme	assay	1013:1017	arg1	reliability					994:1004	the reliability	990:1004	the reliability of the assay by HPLC coupled MS analysis	990:1045	We further confirmed the reliability of the assay by HPLC coupled MS analysis.					
32475593	0	49	theme	monosaccharide	56:69	arg1	analyses					83:90	monosaccharide composition analyses	56:90	monosaccharide composition analyses	56:90	Using a PCR instrument to hydrolyze polysaccharides for monosaccharide composition analyses.					
32475593	0	50	theme	PCR	8:10	arg1	instrument					12:21	a PCR instrument	6:21	a PCR instrument to hydrolyze polysaccharides for monosaccharide composition analyses	6:90	Using a PCR instrument to hydrolyze polysaccharides for monosaccharide composition analyses.					
32475593	2	51	theme	monosaccharide	370:383	arg1	products					385:392	monosaccharide products	370:392	monosaccharide products from up to 96 samples	370:414	To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min.					
32475593	7	52	theme	polysaccharides	1182:1196	arg1	analysis					1144:1151	monosaccharide composition analysis	1117:1151	monosaccharide composition analysis of complex, acidic and basic polysaccharides	1117:1196	In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.					
32475593	6	53	theme	MS	1035:1036	arg1	analysis					1038:1045	HPLC coupled MS analysis	1022:1045	HPLC coupled MS analysis	1022:1045	We further confirmed the reliability of the assay by HPLC coupled MS analysis.					
33357849	3	0	from	ryegrass	907:914	arg1	hemicelluloses					889:902	the original hemicelluloses	876:902	the original hemicelluloses in ryegrass	876:914	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
33357849	3	0	from	ryegrass	907:914	arg1	%					871:871	more than 90 %	858:871	more than 90 % of the original hemicelluloses in ryegrass	858:914	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
33357849	5	1	theme	new	1304:1306	arg1	insights					1308:1315	new insights	1304:1315	new insights into the collaborative utilization of hemicelluloses and cellulose in ryegrass	1304:1394	These results provided new insights into the collaborative utilization of hemicelluloses and cellulose in ryegrass.					
33357849	1	2	from	ryegrass	331:338	arg1	hemicelluloses					301:314	hemicelluloses	301:314	hemicelluloses from perennial ryegrass	301:338	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	2	3	theme	exchange	732:739	arg1	chromatography					741:754	high-performance anion exchange chromatography	709:754	high-performance anion exchange chromatography	709:754	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	2	4	theme	nuclear	802:808	arg1	resonance					819:827	nuclear magnetic resonance	802:827	nuclear magnetic resonance spectra	802:835	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	2	5	theme	chemical	413:420	arg1	composition					422:432	chemical composition	413:432	chemical composition	413:432	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	3	6	theme	ʟ-arabino-	1028:1037	arg1	-ᴅ-xylans					1062:1070	ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans	1028:1070	ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans	1028:1070	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
33357849	0	7	theme	enzymatic	109:117	arg1	hydrolysis					119:128	enzymatic hydrolysis	109:128	enzymatic hydrolysis of cellulose	109:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	4	8	dep	%	1278:1278	arg1	to					1270:1271	to	1270:1271	to	1270:1271	In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1 %.					
33357849	2	9	theme	anion	726:730	arg1	chromatography					741:754	high-performance anion exchange chromatography	709:754	high-performance anion exchange chromatography	709:754	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	3	10	from	%	871:871	arg1	ryegrass					907:914	ryegrass	907:914	ryegrass	907:914	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
33357849	1	11	theme	perennial	321:329	arg1	ryegrass					331:338	perennial ryegrass	321:338	perennial ryegrass	321:338	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	2	12	theme	high-performance	709:724	arg1	chromatography					741:754	high-performance anion exchange chromatography	709:754	high-performance anion exchange chromatography	709:754	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	3	13	theme	hemicellulosic	970:983	arg1	fractions					985:993	all hemicellulosic fractions	966:993	all hemicellulosic fractions obtained	966:1002	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
33357849	1	14	theme	hydrothermal	192:203	arg1	pretreatments					205:217	coupling ultrasonic and hydrothermal pretreatments	168:217	pretreatments	205:217	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	0	15	theme	hydrolysis	119:128	arg1	improvement					94:104	improvement	94:104	improvement of enzymatic hydrolysis of cellulose	94:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	0	15	theme	hydrolysis	119:128	arg1	ryegrass					34:41	perennial ryegrass	24:41	perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose	24:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	0	15	theme	hydrolysis	119:128	arg1	characterization					55:70	Structural characterization	44:70	Structural characterization of hemicelluloses	44:88	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	2	16	theme	water-soluble	452:464	arg1	hemicelluloses					485:498	water-soluble and alkali-soluble hemicelluloses	452:498	water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose	452:640	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	2	17	dep	Fourier	757:763	arg1	transform					765:773	transform	765:773	transform infrared spectroscopy	765:795	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	3	18	theme	original	880:887	arg1	hemicelluloses					889:902	the original hemicelluloses	876:902	the original hemicelluloses in ryegrass	876:914	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
33357849	3	19	from	hemicelluloses	889:902	arg1	ryegrass					907:914	ryegrass	907:914	ryegrass	907:914	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
33357849	2	20	theme	enzymatic	597:605	arg1	efficiency					618:627	the enzymatic hydrolysis efficiency	593:627	the enzymatic hydrolysis efficiency of cellulose	593:640	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	4	21	theme	cellulose	1212:1220	arg1	rate					1243:1246	the cellulose enzymatic hydrolysis rate	1208:1246	the cellulose enzymatic hydrolysis rate of ryegrass	1208:1258	In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1 %.					
33357849	3	22	theme	hemicelluloses	889:902	arg1	hemicelluloses					889:902	the original hemicelluloses	876:902	the original hemicelluloses in ryegrass	876:914	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
33357849	3	22	theme	hemicelluloses	889:902	arg1	%					871:871	more than 90 %	858:871	more than 90 % of the original hemicelluloses in ryegrass	858:914	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
33357849	2	23	theme	resonance	819:827	arg1	spectra					829:835	nuclear magnetic resonance spectra	802:835	nuclear magnetic resonance spectra	802:835	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	1	24	theme	sequential	224:233	arg1	post-extractions					242:257	sequential alkali post-extractions	224:257	sequential alkali post-extractions	224:257	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	0	25	theme	perennial	24:32	arg1	improvement					94:104	improvement	94:104	improvement of enzymatic hydrolysis of cellulose	94:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	0	25	theme	perennial	24:32	arg1	ryegrass					34:41	perennial ryegrass	24:41	perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose	24:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	0	25	theme	perennial	24:32	arg1	characterization					55:70	Structural characterization	44:70	Structural characterization of hemicelluloses	44:88	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	4	26	theme	effective	1127:1135	arg1	removal					1137:1143	the effective removal	1123:1143	the effective removal of amorphous hemicelluloses	1123:1171	In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1 %.					
33357849	2	27	theme	magnetic	810:817	arg1	resonance					819:827	nuclear magnetic resonance	802:827	nuclear magnetic resonance spectra	802:835	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	1	28	dep	treatment	158:166	arg1	ultrasonic					177:186	ultrasonic	177:186	ultrasonic	177:186	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	1	28	dep	treatment	158:166	arg1	pretreatments					205:217	coupling ultrasonic and hydrothermal pretreatments	168:217	pretreatments	205:217	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	1	29	theme	alkali	235:240	arg1	post-extractions					242:257	sequential alkali post-extractions	224:257	sequential alkali post-extractions	224:257	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	2	30	dep	transform	765:773	arg1	infrared					775:782	infrared	775:782	transform infrared spectroscopy	765:795	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	0	31	theme	cellulose	133:141	arg1	hydrolysis					119:128	enzymatic hydrolysis	109:128	enzymatic hydrolysis of cellulose	109:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	4	32	theme	amorphous	1148:1156	arg1	hemicelluloses					1158:1171	amorphous hemicelluloses	1148:1171	amorphous hemicelluloses	1148:1171	In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1 %.					
33357849	1	33	theme	enzymatic	356:364	arg1	efficiency					377:386	the enzymatic hydrolysis efficiency	352:386	the enzymatic hydrolysis efficiency of cellulose	352:399	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	2	34	theme	hemicelluloses	485:498	arg1	yield					406:410	The yield	402:410	The yield	402:410	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	2	34	theme	hemicelluloses	485:498	arg1	composition					422:432	chemical composition	413:432	chemical composition	413:432	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	2	34	theme	hemicelluloses	485:498	arg1	structure					439:447	structure	439:447	structure	439:447	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	0	35	theme	ryegrass	34:41	arg1	treatment					11:19	treatment	11:19	treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose	11:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	1	36	theme	hydrolysis	366:375	arg1	efficiency					377:386	the enzymatic hydrolysis efficiency	352:386	the enzymatic hydrolysis efficiency of cellulose	352:399	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	2	37	theme	hydrothermal	518:529	arg1	supernatant					531:541	the hydrothermal supernatant	514:541	the hydrothermal supernatant	514:541	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	2	38	theme	hydrolysis	607:616	arg1	efficiency					618:627	the enzymatic hydrolysis efficiency	593:627	the enzymatic hydrolysis efficiency of cellulose	593:640	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	2	39	theme	pretreated	562:571	arg1	ryegrass					573:580	hydrothermally pretreated ryegrass	547:580	hydrothermally pretreated ryegrass	547:580	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	2	40	theme	cellulose	632:640	arg1	supernatant					531:541	the hydrothermal supernatant	514:541	the hydrothermal supernatant	514:541	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	2	40	theme	cellulose	632:640	arg1	efficiency					618:627	the enzymatic hydrolysis efficiency	593:627	the enzymatic hydrolysis efficiency of cellulose	593:640	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	2	40	theme	cellulose	632:640	arg1	ryegrass					573:580	hydrothermally pretreated ryegrass	547:580	hydrothermally pretreated ryegrass	547:580	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	0	41	theme	Structural	44:53	arg1	ryegrass					34:41	perennial ryegrass	24:41	perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose	24:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	0	41	theme	Structural	44:53	arg1	characterization					55:70	Structural characterization	44:70	Structural characterization of hemicelluloses	44:88	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	5	42	theme	hemicelluloses	1355:1368	arg1	utilization					1340:1350	the collaborative utilization	1322:1350	the collaborative utilization of hemicelluloses and cellulose in ryegrass	1322:1394	These results provided new insights into the collaborative utilization of hemicelluloses and cellulose in ryegrass.					
33357849	0	43	dep	ryegrass	34:41	arg1	improvement					94:104	improvement	94:104	improvement of enzymatic hydrolysis of cellulose	94:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	0	43	dep	ryegrass	34:41	arg1	ryegrass					34:41	perennial ryegrass	24:41	perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose	24:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	0	43	dep	ryegrass	34:41	arg1	characterization					55:70	Structural characterization	44:70	Structural characterization of hemicelluloses	44:88	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	1	44	with	treatment	158:166	arg1	post-extractions					242:257	sequential alkali post-extractions	224:257	sequential alkali post-extractions	224:257	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	1	45	theme	cellulose	391:399	arg1	efficiency					377:386	the enzymatic hydrolysis efficiency	352:386	the enzymatic hydrolysis efficiency of cellulose	352:399	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	2	46	theme	permeation	683:692	arg1	chromatograph					694:706	gel permeation chromatograph	679:706	gel permeation chromatograph	679:706	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	5	47	from	utilization	1340:1350	arg1	ryegrass					1387:1394	ryegrass	1387:1394	ryegrass	1387:1394	These results provided new insights into the collaborative utilization of hemicelluloses and cellulose in ryegrass.					
33357849	4	48	theme	hemicelluloses	1158:1171	arg1	lignin					1177:1182	lignin	1177:1182	lignin	1177:1182	In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1 %.					
33357849	4	48	theme	hemicelluloses	1158:1171	arg1	removal					1137:1143	the effective removal	1123:1143	the effective removal of amorphous hemicelluloses	1123:1171	In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1 %.					
33357849	2	49	theme	gel	679:681	arg1	chromatograph					694:706	gel permeation chromatograph	679:706	gel permeation chromatograph	679:706	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	1	50	theme	integrated	147:156	arg1	treatment					158:166	An integrated treatment	144:166	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions	144:257	An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose.					
33357849	0	51	theme	hemicelluloses	75:88	arg1	improvement					94:104	improvement	94:104	improvement of enzymatic hydrolysis of cellulose	94:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	0	51	theme	hemicelluloses	75:88	arg1	ryegrass					34:41	perennial ryegrass	24:41	perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose	24:141	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	0	51	theme	hemicelluloses	75:88	arg1	characterization					55:70	Structural characterization	44:70	Structural characterization of hemicelluloses	44:88	Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.					
33357849	2	52	theme	alkali-soluble	470:483	arg1	hemicelluloses					485:498	water-soluble and alkali-soluble hemicelluloses	452:498	water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose	452:640	The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra.					
33357849	5	53	theme	collaborative	1326:1338	arg1	utilization					1340:1350	the collaborative utilization	1322:1350	the collaborative utilization of hemicelluloses and cellulose in ryegrass	1322:1394	These results provided new insights into the collaborative utilization of hemicelluloses and cellulose in ryegrass.					
33357849	4	54	theme	ryegrass	1251:1258	arg1	rate					1243:1246	the cellulose enzymatic hydrolysis rate	1208:1246	the cellulose enzymatic hydrolysis rate of ryegrass	1208:1258	In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1 %.					
33357849	5	55	theme	cellulose	1374:1382	arg1	utilization					1340:1350	the collaborative utilization	1322:1350	the collaborative utilization of hemicelluloses and cellulose in ryegrass	1322:1394	These results provided new insights into the collaborative utilization of hemicelluloses and cellulose in ryegrass.					
33357849	4	56	theme	hydrolysis	1232:1241	arg1	rate					1243:1246	the cellulose enzymatic hydrolysis rate	1208:1246	the cellulose enzymatic hydrolysis rate of ryegrass	1208:1258	In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1 %.					
33357849	4	57	theme	enzymatic	1222:1230	arg1	rate					1243:1246	the cellulose enzymatic hydrolysis rate	1208:1246	the cellulose enzymatic hydrolysis rate of ryegrass	1208:1258	In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1 %.					
33357849	3	58	theme	4-O-methyl-ᴅ-glucurono	1039:1060	arg1	-ᴅ-xylans					1062:1070	ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans	1028:1070	ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans	1028:1070	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
33357849	3	59	theme	integrated	941:950	arg1	treatment					952:960	the integrated treatment	937:960	the integrated treatment	937:960	Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans.					
34334021	4	0	theme	structural	829:838	arg1	systems					629:635	lever systems	623:635	lever systems for locomotion	623:650	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	4	0	theme	structural	829:838	arg1	colours					840:846	structural colours	829:846	structural colours	829:846	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	1	1	theme	materials	137:145	arg1	materials					137:145	materials	137:145	materials differing in composition and processing to provide the desired functionality	137:222	Engineered systems are typically based on a large variety of materials differing in composition and processing to provide the desired functionality.					
34334021	1	1	theme	materials	137:145	arg1	variety					126:132	a large variety	118:132	a large variety of materials differing in composition and processing to provide the desired functionality	118:222	Engineered systems are typically based on a large variety of materials differing in composition and processing to provide the desired functionality.					
34334021	6	2	theme	theme	1101:1105	arg1	issue					1107:1111	the theme issue	1097:1111	the theme issue	1097:1111	This article is part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'.					
34334021	2	3	theme	minimal	325:331	arg1	changes					347:353	minimal compositional changes	325:353	minimal compositional changes	325:353	Nature, however, has evolved materials that are used for a wide range of functional challenges with minimal compositional changes.					
34334021	4	4	theme	interlocking	735:746	arg1	structures					759:768	auxiliary structures	749:768	auxiliary structures for the transmission	749:789	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	6	5	theme	emerging	1177:1184	arg1	part					1200:1203	part 1	1200:1205	part 1	1200:1205	This article is part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'.					
34334021	6	5	theme	emerging	1177:1184	arg1	technologies					1186:1197	emerging technologies	1177:1197	emerging technologies (part 1)	1177:1206	This article is part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'.					
34334021	3	6	theme	protein	493:499	arg1	combination					470:480	a combination	468:480	a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals	468:561	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	2	7	used	used	273:276	arg2	materials					254:262	materials	254:262	materials that are used for a wide range of functional challenges with minimal compositional changes	254:353	Nature, however, has evolved materials that are used for a wide range of functional challenges with minimal compositional changes.					
34334021	3	8	theme	small	512:516	arg1	cross-linkers					537:549	organic cross-linkers	529:549	organic cross-linkers	529:549	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	8	theme	small	512:516	arg1	minerals					554:561	minerals	554:561	minerals	554:561	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	8	theme	small	512:516	arg1	amounts					518:524	small amounts	512:524	small amounts	512:524	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	4	9	theme	piercing	713:720	arg1	structures					759:768	auxiliary structures	749:768	auxiliary structures for the transmission	749:789	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	6	10	theme	advanced	1154:1161	arg1	materials					1163:1171	Bio-derived and bioinspired sustainable advanced materials	1114:1171	part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'	1089:1207	This article is part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'.					
34334021	2	11	theme	compositional	333:345	arg1	changes					347:353	minimal compositional changes	325:353	minimal compositional changes	325:353	Nature, however, has evolved materials that are used for a wide range of functional challenges with minimal compositional changes.					
34334021	4	12	theme	adverse	669:675	arg1	influences					691:700	adverse environmental influences	669:700	adverse environmental influences	669:700	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	6	13	dep	part	1089:1092	arg1	materials					1163:1171	Bio-derived and bioinspired sustainable advanced materials	1114:1171	part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'	1089:1207	This article is part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'.					
34334021	3	14	theme	chitin	485:490	arg1	combination					470:480	a combination	468:480	a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals	468:561	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	15	theme	other	406:410	arg1	crustaceans					443:453	crustaceans	443:453	crustaceans	443:453	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	15	theme	other	406:410	arg1	arthropods					412:421	other arthropods	406:421	other arthropods such as insects and crustaceans	406:453	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	15	theme	other	406:410	arg1	insects					431:437	insects	431:437	insects	431:437	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	4	16	theme	cutting	723:729	arg1	structures					759:768	auxiliary structures	749:768	auxiliary structures for the transmission	749:789	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	3	17	theme	arthropods	412:421	arg1	cuticle					372:378	The exoskeletal cuticle	356:378	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans,	356:454	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	0	18	theme	spider	4:9	arg1	cuticle					11:17	The spider cuticle	0:17	The spider cuticle: a remarkable material toolbox for functional diversity.	0:74	The spider cuticle: a remarkable material toolbox for functional diversity.					
34334021	5	19	theme	functions	1062:1070	arg1	diversity					1049:1057	a diversity	1047:1057	a diversity of functions	1047:1070	This paper illustrates the 'design space' of a single type of composite with varying internal architecture and its remarkable capability to serve a diversity of functions.					
34334021	4	20	theme	information	816:826	arg1	filtering					795:803	filtering	795:803	filtering of sensory information	795:826	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	4	20	theme	information	816:826	arg1	structures					759:768	auxiliary structures	749:768	auxiliary structures for the transmission	749:789	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	1	21	theme	Engineered	76:85	arg1	systems					87:93	Engineered systems	76:93	Engineered systems	76:93	Engineered systems are typically based on a large variety of materials differing in composition and processing to provide the desired functionality.					
34334021	4	22	theme	lever	623:627	arg1	tools					703:707	tools	703:707	tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information	703:826	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	4	22	theme	lever	623:627	arg1	systems					629:635	lever systems	623:635	lever systems for locomotion	623:650	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	4	22	theme	lever	623:627	arg1	colours					840:846	structural colours	829:846	structural colours	829:846	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	4	22	theme	lever	623:627	arg1	lenses					861:866	transparent lenses	849:866	transparent lenses	849:866	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	4	22	theme	lever	623:627	arg1	protection					653:662	protection	653:662	protection from adverse environmental influences	653:700	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	6	23	theme	sustainable	1142:1152	arg1	materials					1163:1171	Bio-derived and bioinspired sustainable advanced materials	1114:1171	part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'	1089:1207	This article is part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'.					
34334021	4	24	theme	sensory	808:814	arg1	information					816:826	sensory information	808:826	sensory information	808:826	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	2	25	theme	wide	284:287	arg1	range					289:293	a wide range	282:293	a wide range of functional challenges with minimal compositional changes	282:353	Nature, however, has evolved materials that are used for a wide range of functional challenges with minimal compositional changes.					
34334021	5	26	theme	single	948:953	arg1	type					955:958	a single type	946:958	a single type of composite	946:971	This paper illustrates the 'design space' of a single type of composite with varying internal architecture and its remarkable capability to serve a diversity of functions.					
34334021	4	27	from	influences	691:700	arg1	systems					629:635	lever systems	623:635	lever systems for locomotion	623:650	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	4	27	from	influences	691:700	arg1	protection					653:662	protection	653:662	protection from adverse environmental influences	653:700	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	3	28	theme	water	502:506	arg1	combination					470:480	a combination	468:480	a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals	468:561	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	29	theme	amounts	518:524	arg1	combination					470:480	a combination	468:480	a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals	468:561	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	5	30	theme	remarkable	1016:1025	arg1	capability					1027:1036	its remarkable capability	1012:1036	its remarkable capability	1012:1036	This paper illustrates the 'design space' of a single type of composite with varying internal architecture and its remarkable capability to serve a diversity of functions.					
34334021	0	31	theme	remarkable	22:31	arg1	toolbox					42:48	a remarkable material toolbox	20:48	The spider cuticle: a remarkable material toolbox for functional diversity.	0:74	The spider cuticle: a remarkable material toolbox for functional diversity.					
34334021	5	32	theme	type	955:958	arg1	space					936:940	the 'design space'	924:941	the 'design space' of a single type of composite	924:971	This paper illustrates the 'design space' of a single type of composite with varying internal architecture and its remarkable capability to serve a diversity of functions.					
34334021	3	33	theme	cross-linkers	537:549	arg1	cross-linkers					537:549	organic cross-linkers	529:549	organic cross-linkers	529:549	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	33	theme	cross-linkers	537:549	arg1	water					502:506	water	502:506	water	502:506	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	33	theme	cross-linkers	537:549	arg1	minerals					554:561	minerals	554:561	minerals	554:561	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	33	theme	cross-linkers	537:549	arg1	amounts					518:524	small amounts	512:524	small amounts	512:524	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	33	theme	cross-linkers	537:549	arg1	chitin					485:490	chitin	485:490	chitin	485:490	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	33	theme	cross-linkers	537:549	arg1	protein					493:499	protein	493:499	protein	493:499	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	6	34	theme	bioinspired	1130:1140	arg1	materials					1163:1171	Bio-derived and bioinspired sustainable advanced materials	1114:1171	part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'	1089:1207	This article is part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'.					
34334021	4	35	theme	mechanical	589:598	arg1	structures					608:617	mechanical support structures	589:617	mechanical support structures	589:617	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	5	36	theme	composite	963:971	arg1	type					955:958	a single type	946:958	a single type of composite	946:971	This paper illustrates the 'design space' of a single type of composite with varying internal architecture and its remarkable capability to serve a diversity of functions.					
34334021	1	37	theme	desired	202:208	arg1	functionality					210:222	the desired functionality	198:222	the desired functionality	198:222	Engineered systems are typically based on a large variety of materials differing in composition and processing to provide the desired functionality.					
34334021	3	38	theme	spiders	383:389	arg1	cuticle					372:378	The exoskeletal cuticle	356:378	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans,	356:454	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	2	39	with	challenges	309:318	arg1	changes					347:353	minimal compositional changes	325:353	minimal compositional changes	325:353	Nature, however, has evolved materials that are used for a wide range of functional challenges with minimal compositional changes.					
34334021	5	40	theme	design	929:934	arg1	space					936:940	the 'design space'	924:941	the 'design space' of a single type of composite	924:971	This paper illustrates the 'design space' of a single type of composite with varying internal architecture and its remarkable capability to serve a diversity of functions.					
34334021	0	41	theme	material	33:40	arg1	toolbox					42:48	a remarkable material toolbox	20:48	The spider cuticle: a remarkable material toolbox for functional diversity.	0:74	The spider cuticle: a remarkable material toolbox for functional diversity.					
34334021	6	42	theme	Bio-derived	1114:1124	arg1	materials					1163:1171	Bio-derived and bioinspired sustainable advanced materials	1114:1171	part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'	1089:1207	This article is part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'.					
34334021	4	43	theme	transparent	849:859	arg1	systems					629:635	lever systems	623:635	lever systems for locomotion	623:650	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	4	43	theme	transparent	849:859	arg1	lenses					861:866	transparent lenses	849:866	transparent lenses	849:866	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	5	44	theme	internal	986:993	arg1	architecture					995:1006	internal architecture	986:1006	internal architecture	986:1006	This paper illustrates the 'design space' of a single type of composite with varying internal architecture and its remarkable capability to serve a diversity of functions.					
34334021	3	45	theme	organic	529:535	arg1	cross-linkers					537:549	organic cross-linkers	529:549	organic cross-linkers	529:549	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	2	46	theme	challenges	309:318	arg1	range					289:293	a wide range	282:293	a wide range of functional challenges with minimal compositional changes	282:353	Nature, however, has evolved materials that are used for a wide range of functional challenges with minimal compositional changes.					
34334021	4	47	theme	auxiliary	749:757	arg1	structures					759:768	auxiliary structures	749:768	auxiliary structures for the transmission	749:789	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	4	48	theme	light	872:876	arg1	manipulation					878:889	light manipulation	872:889	light manipulation	872:889	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	2	49	theme	functional	298:307	arg1	challenges					309:318	functional challenges	298:318	functional challenges with minimal compositional changes	298:353	Nature, however, has evolved materials that are used for a wide range of functional challenges with minimal compositional changes.					
34334021	6	50	theme	issue	1107:1111	arg1	part					1089:1092	part	1089:1092	part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'	1089:1207	This article is part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'.					
34334021	0	51	dep	cuticle	11:17	arg1	toolbox					42:48	a remarkable material toolbox	20:48	The spider cuticle: a remarkable material toolbox for functional diversity.	0:74	The spider cuticle: a remarkable material toolbox for functional diversity.					
34334021	1	52	theme	large	120:124	arg1	materials					137:145	materials	137:145	materials differing in composition and processing to provide the desired functionality	137:222	Engineered systems are typically based on a large variety of materials differing in composition and processing to provide the desired functionality.					
34334021	1	52	theme	large	120:124	arg1	variety					126:132	a large variety	118:132	a large variety of materials differing in composition and processing to provide the desired functionality	118:222	Engineered systems are typically based on a large variety of materials differing in composition and processing to provide the desired functionality.					
34334021	4	53	theme	environmental	677:689	arg1	influences					691:700	adverse environmental influences	669:700	adverse environmental influences	669:700	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	0	54	theme	functional	54:63	arg1	diversity					65:73	functional diversity	54:73	functional diversity	54:73	The spider cuticle: a remarkable material toolbox for functional diversity.					
34334021	3	55	theme	minerals	554:561	arg1	cross-linkers					537:549	organic cross-linkers	529:549	organic cross-linkers	529:549	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	55	theme	minerals	554:561	arg1	water					502:506	water	502:506	water	502:506	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	55	theme	minerals	554:561	arg1	minerals					554:561	minerals	554:561	minerals	554:561	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	55	theme	minerals	554:561	arg1	amounts					518:524	small amounts	512:524	small amounts	512:524	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	55	theme	minerals	554:561	arg1	chitin					485:490	chitin	485:490	chitin	485:490	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	3	55	theme	minerals	554:561	arg1	protein					493:499	protein	493:499	protein	493:499	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	4	56	theme	support	600:606	arg1	structures					608:617	mechanical support structures	589:617	mechanical support structures	589:617	Spiders use it to obtain mechanical support structures and lever systems for locomotion, protection from adverse environmental influences, tools for piercing, cutting and interlocking, auxiliary structures for the transmission and filtering of sensory information, structural colours, transparent lenses for light manipulation and more.					
34334021	3	57	theme	exoskeletal	360:370	arg1	cuticle					372:378	The exoskeletal cuticle	356:378	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans,	356:454	The exoskeletal cuticle of spiders, as well as of other arthropods such as insects and crustaceans, is based on a combination of chitin, protein, water and small amounts of organic cross-linkers or minerals.					
34334021	6	58	link	Bio-derived	1114:1124	arg1	materials					1163:1171	Bio-derived and bioinspired sustainable advanced materials	1114:1171	part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'	1089:1207	This article is part of the theme issue 'Bio-derived and bioinspired sustainable advanced materials for emerging technologies (part 1)'.					
32135259	3	0	dep	infrared	388:395	arg1	FTIR					398:401	FTIR	398:401	FTIR	398:401	Fourier transform infrared (FTIR) spectroscopy provided the evidence about removal of hemicellulose and lignin.					
32135259	1	1	theme	plant	151:155	arg1	fiber					164:168	the plant dunchi fiber	147:168	the plant dunchi fiber	147:168	In the current study, cellulose was extracted from the plant dunchi fiber by using an ecofriendly method followed by preparation of nanocellulose.					
32135259	9	2	theme	TGA	970:972	arg1	curve					974:978	The TGA curve	966:978	The TGA curve	966:978	The TGA curve showed the thermal degradation pattern of the cellulosic material and it was closed to the thermal behavior of pure cellulose.					
32135259	4	3	theme	fibers	542:547	arg1	surface					515:521	the surface	511:521	the surface of lignocellulosic fibers	511:547	The morphological changes in the surface of lignocellulosic fibers were studied through scanning electron microscopy (SEM).					
32135259	11	4	theme	aspect	1294:1299	arg1	ratio					1301:1305	an average aspect ratio	1283:1305	an average aspect ratio of 10.45 ± 3.44	1283:1321	Transmission electron microscopy (TEM) showed the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44.					
32135259	1	5	theme	dunchi	157:162	arg1	fiber					164:168	the plant dunchi fiber	147:168	the plant dunchi fiber	147:168	In the current study, cellulose was extracted from the plant dunchi fiber by using an ecofriendly method followed by preparation of nanocellulose.					
32135259	5	6	theme	crystallinity	662:674	arg1	degree					652:657	the degree	648:657	the degree of crystallinity of extracted cellulosic material	648:707	X-ray Diffraction (XRD) analysis measured the degree of crystallinity of extracted cellulosic material.					
32135259	4	7	theme	lignocellulosic	526:540	arg1	fibers					542:547	lignocellulosic fibers	526:547	lignocellulosic fibers	526:547	The morphological changes in the surface of lignocellulosic fibers were studied through scanning electron microscopy (SEM).					
32135259	5	8	theme	extracted	679:687	arg1	material					700:707	extracted cellulosic material	679:707	extracted cellulosic material	679:707	X-ray Diffraction (XRD) analysis measured the degree of crystallinity of extracted cellulosic material.					
32135259	2	9	theme	chlorine-free	290:302	arg1	bleaching					304:312	chlorine-free bleaching	290:312	chlorine-free bleaching	290:312	The procedure involved an alkali treatment and chlorine-free bleaching for removal of lignin and hemicelluloses from material.					
32135259	0	10	from	fiber	59:63	arg1	extraction					23:32	Environmentally benign extraction	0:32	Environmentally benign extraction of cellulose from dunchi fiber for nanocellulose fabrication.	0:94	Environmentally benign extraction of cellulose from dunchi fiber for nanocellulose fabrication.					
32135259	5	11	theme	cellulosic	689:698	arg1	material					700:707	extracted cellulosic material	679:707	extracted cellulosic material	679:707	X-ray Diffraction (XRD) analysis measured the degree of crystallinity of extracted cellulosic material.					
32135259	4	12	theme	morphological	486:498	arg1	changes					500:506	The morphological changes	482:506	The morphological changes in the surface of lignocellulosic fibers	482:547	The morphological changes in the surface of lignocellulosic fibers were studied through scanning electron microscopy (SEM).					
32135259	12	13	theme	renewable	1384:1392	arg1	source					1394:1399	a renewable source	1382:1399	a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials	1382:1511	In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials.					
32135259	7	14	theme	Crystal	778:784	arg1	thickness					786:794	Crystal thickness	778:794	Crystal thickness	778:794	Crystal thickness was determined by Scherrer equation and its value was found to be 40.07 Å.					
32135259	11	15	theme	nanocellulose	1264:1276	arg1	existence					1251:1259	the existence	1247:1259	the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44	1247:1321	Transmission electron microscopy (TEM) showed the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44.					
32135259	2	16	theme	alkali	269:274	arg1	treatment					276:284	alkali treatment	269:284	alkali treatment	269:284	The procedure involved an alkali treatment and chlorine-free bleaching for removal of lignin and hemicelluloses from material.					
32135259	9	17	theme	thermal	1071:1077	arg1	behavior					1079:1086	the thermal behavior	1067:1086	the thermal behavior of pure cellulose	1067:1104	The TGA curve showed the thermal degradation pattern of the cellulosic material and it was closed to the thermal behavior of pure cellulose.					
32135259	3	18	theme	hemicellulose	456:468	arg1	removal					445:451	removal	445:451	removal of hemicellulose and lignin	445:479	Fourier transform infrared (FTIR) spectroscopy provided the evidence about removal of hemicellulose and lignin.					
32135259	0	19	theme	benign	16:21	arg1	extraction					23:32	Environmentally benign extraction	0:32	Environmentally benign extraction of cellulose from dunchi fiber for nanocellulose fabrication.	0:94	Environmentally benign extraction of cellulose from dunchi fiber for nanocellulose fabrication.					
32135259	4	20	from	changes	500:506	arg1	surface					515:521	the surface	511:521	the surface of lignocellulosic fibers	511:547	The morphological changes in the surface of lignocellulosic fibers were studied through scanning electron microscopy (SEM).					
32135259	2	21	from	material	360:367	arg1	removal					318:324	removal	318:324	removal of lignin and hemicelluloses from material	318:367	The procedure involved an alkali treatment and chlorine-free bleaching for removal of lignin and hemicelluloses from material.					
32135259	11	22	theme	Transmission	1201:1212	arg1	TEM					1235:1237	TEM	1235:1237	TEM	1235:1237	Transmission electron microscopy (TEM) showed the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44.					
32135259	11	22	theme	Transmission	1201:1212	arg1	microscopy					1223:1232	Transmission electron microscopy	1201:1232	Transmission electron microscopy (TEM)	1201:1238	Transmission electron microscopy (TEM) showed the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44.					
32135259	6	23	theme	Segal	719:723	arg1	method					725:730	Segal method	719:730	Segal method	719:730	By using Segal method, the degree of crystallinity was found 66.7%.					
32135259	11	24	with	existence	1251:1259	arg1	ratio					1301:1305	an average aspect ratio	1283:1305	an average aspect ratio of 10.45 ± 3.44	1283:1321	Transmission electron microscopy (TEM) showed the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44.					
32135259	0	25	theme	Environmentally	0:14	arg1	extraction					23:32	Environmentally benign extraction	0:32	Environmentally benign extraction of cellulose from dunchi fiber for nanocellulose fabrication.	0:94	Environmentally benign extraction of cellulose from dunchi fiber for nanocellulose fabrication.					
32135259	12	26	from	materials	1503:1511	arg1	range					1468:1472	a wide range	1461:1472	a wide range of applications in composite materials	1461:1511	In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials.					
32135259	5	27	theme	X-ray	606:610	arg1	XRD					625:627	XRD	625:627	XRD	625:627	X-ray Diffraction (XRD) analysis measured the degree of crystallinity of extracted cellulosic material.					
32135259	5	27	theme	X-ray	606:610	arg1	Diffraction					612:622	X-ray Diffraction	606:622	X-ray Diffraction (XRD) analysis	606:637	X-ray Diffraction (XRD) analysis measured the degree of crystallinity of extracted cellulosic material.					
32135259	11	28	theme	electron	1214:1221	arg1	TEM					1235:1237	TEM	1235:1237	TEM	1235:1237	Transmission electron microscopy (TEM) showed the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44.					
32135259	11	28	theme	electron	1214:1221	arg1	microscopy					1223:1232	Transmission electron microscopy	1201:1232	Transmission electron microscopy (TEM)	1201:1238	Transmission electron microscopy (TEM) showed the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44.					
32135259	11	29	theme	average	1286:1292	arg1	ratio					1301:1305	an average aspect ratio	1283:1305	an average aspect ratio of 10.45 ± 3.44	1283:1321	Transmission electron microscopy (TEM) showed the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44.					
32135259	3	30	theme	infrared	388:395	arg1	spectroscopy					404:415	infrared (FTIR) spectroscopy	388:415	infrared (FTIR) spectroscopy	388:415	Fourier transform infrared (FTIR) spectroscopy provided the evidence about removal of hemicellulose and lignin.					
32135259	4	31	theme	electron	579:586	arg1	microscopy					588:597	scanning electron microscopy	570:597	scanning electron microscopy (SEM)	570:603	The morphological changes in the surface of lignocellulosic fibers were studied through scanning electron microscopy (SEM).					
32135259	4	31	theme	electron	579:586	arg1	SEM					600:602	SEM	600:602	SEM	600:602	The morphological changes in the surface of lignocellulosic fibers were studied through scanning electron microscopy (SEM).					
32135259	5	32	theme	Diffraction	612:622	arg1	analysis					630:637	X-ray Diffraction (XRD) analysis	606:637	X-ray Diffraction (XRD) analysis	606:637	X-ray Diffraction (XRD) analysis measured the degree of crystallinity of extracted cellulosic material.					
32135259	1	33	theme	current	103:109	arg1	study					111:115	the current study	99:115	the current study	99:115	In the current study, cellulose was extracted from the plant dunchi fiber by using an ecofriendly method followed by preparation of nanocellulose.					
32135259	9	34	theme	pure	1091:1094	arg1	cellulose					1096:1104	pure cellulose	1091:1104	pure cellulose	1091:1104	The TGA curve showed the thermal degradation pattern of the cellulosic material and it was closed to the thermal behavior of pure cellulose.					
32135259	1	35	theme	ecofriendly	182:192	arg1	method					194:199	an ecofriendly method	179:199	an ecofriendly method followed by preparation of nanocellulose	179:240	In the current study, cellulose was extracted from the plant dunchi fiber by using an ecofriendly method followed by preparation of nanocellulose.					
32135259	12	36	theme	applications	1477:1488	arg1	range					1468:1472	a wide range	1461:1472	a wide range of applications in composite materials	1461:1511	In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials.					
32135259	9	37	theme	cellulose	1096:1104	arg1	behavior					1079:1086	the thermal behavior	1067:1086	the thermal behavior of pure cellulose	1067:1104	The TGA curve showed the thermal degradation pattern of the cellulosic material and it was closed to the thermal behavior of pure cellulose.					
32135259	12	38	from	range	1468:1472	arg1	materials					1503:1511	composite materials	1493:1511	composite materials	1493:1511	In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials.					
32135259	7	39	theme	Scherrer	814:821	arg1	equation					823:830	Scherrer equation	814:830	Scherrer equation	814:830	Crystal thickness was determined by Scherrer equation and its value was found to be 40.07 Å.					
32135259	12	40	theme	dunchi	1339:1344	arg1	fiber					1346:1350	dunchi fiber	1339:1350	dunchi fiber	1339:1350	In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials.					
32135259	0	41	theme	cellulose	37:45	arg1	extraction					23:32	Environmentally benign extraction	0:32	Environmentally benign extraction of cellulose from dunchi fiber for nanocellulose fabrication.	0:94	Environmentally benign extraction of cellulose from dunchi fiber for nanocellulose fabrication.					
32135259	9	42	theme	thermal	991:997	arg1	pattern					1011:1017	the thermal degradation pattern	987:1017	the thermal degradation pattern of the cellulosic material	987:1044	The TGA curve showed the thermal degradation pattern of the cellulosic material and it was closed to the thermal behavior of pure cellulose.					
32135259	12	43	theme	composite	1493:1501	arg1	materials					1503:1511	composite materials	1493:1511	composite materials	1493:1511	In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials.					
32135259	12	44	from	applications	1477:1488	arg1	materials					1503:1511	composite materials	1493:1511	composite materials	1493:1511	In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials.					
32135259	8	45	theme	commercial	921:930	arg1	MCC					960:962	MCC	960:962	MCC	960:962	The values were closed to the values observed for commercial microcrystalline cellulose (MCC).					
32135259	8	45	theme	commercial	921:930	arg1	cellulose					949:957	commercial microcrystalline cellulose	921:957	commercial microcrystalline cellulose (MCC)	921:963	The values were closed to the values observed for commercial microcrystalline cellulose (MCC).					
32135259	3	46	theme	lignin	474:479	arg1	removal					445:451	removal	445:451	removal of hemicellulose and lignin	445:479	Fourier transform infrared (FTIR) spectroscopy provided the evidence about removal of hemicellulose and lignin.					
32135259	9	47	theme	degradation	999:1009	arg1	pattern					1011:1017	the thermal degradation pattern	987:1017	the thermal degradation pattern of the cellulosic material	987:1044	The TGA curve showed the thermal degradation pattern of the cellulosic material and it was closed to the thermal behavior of pure cellulose.					
32135259	10	48	theme	acid	1146:1149	arg1	hydrolysis					1151:1160	acid hydrolysis	1146:1160	acid hydrolysis from the obtained cellulosic material	1146:1198	Finally, nanocellulose was produced by acid hydrolysis from the obtained cellulosic material.					
32135259	5	49	theme	material	700:707	arg1	crystallinity					662:674	crystallinity	662:674	crystallinity of extracted cellulosic material	662:707	X-ray Diffraction (XRD) analysis measured the degree of crystallinity of extracted cellulosic material.					
32135259	0	50	theme	dunchi	52:57	arg1	fiber					59:63	dunchi fiber	52:63	dunchi fiber for nanocellulose fabrication	52:93	Environmentally benign extraction of cellulose from dunchi fiber for nanocellulose fabrication.					
32135259	4	51	theme	scanning	570:577	arg1	microscopy					588:597	scanning electron microscopy	570:597	scanning electron microscopy (SEM)	570:603	The morphological changes in the surface of lignocellulosic fibers were studied through scanning electron microscopy (SEM).					
32135259	4	51	theme	scanning	570:577	arg1	SEM					600:602	SEM	600:602	SEM	600:602	The morphological changes in the surface of lignocellulosic fibers were studied through scanning electron microscopy (SEM).					
32135259	10	52	from	material	1191:1198	arg1	hydrolysis					1151:1160	acid hydrolysis	1146:1160	acid hydrolysis from the obtained cellulosic material	1146:1198	Finally, nanocellulose was produced by acid hydrolysis from the obtained cellulosic material.					
32135259	12	53	contain	has	1352:1354	arg1	fiber					1346:1350	dunchi fiber	1339:1350	dunchi fiber	1339:1350	In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials.					
32135259	12	53	contain	has	1352:1354	arg2	potential					1358:1366	a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials	1356:1511	a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials	1356:1511	In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials.					
32135259	11	54	theme	10.45 ± 3.44	1310:1321	arg1	ratio					1301:1305	an average aspect ratio	1283:1305	an average aspect ratio of 10.45 ± 3.44	1283:1321	Transmission electron microscopy (TEM) showed the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44.					
32135259	2	55	theme	hemicelluloses	340:353	arg1	removal					318:324	removal	318:324	removal of lignin and hemicelluloses from material	318:367	The procedure involved an alkali treatment and chlorine-free bleaching for removal of lignin and hemicelluloses from material.					
32135259	3	56	dep	Fourier	370:376	arg1	transform					378:386	transform	378:386	transform infrared (FTIR) spectroscopy	378:415	Fourier transform infrared (FTIR) spectroscopy provided the evidence about removal of hemicellulose and lignin.					
32135259	8	57	theme	microcrystalline	932:947	arg1	MCC					960:962	MCC	960:962	MCC	960:962	The values were closed to the values observed for commercial microcrystalline cellulose (MCC).					
32135259	8	57	theme	microcrystalline	932:947	arg1	cellulose					949:957	commercial microcrystalline cellulose	921:957	commercial microcrystalline cellulose (MCC)	921:963	The values were closed to the values observed for commercial microcrystalline cellulose (MCC).					
32135259	10	58	theme	cellulosic	1180:1189	arg1	material					1191:1198	the obtained cellulosic material	1167:1198	the obtained cellulosic material	1167:1198	Finally, nanocellulose was produced by acid hydrolysis from the obtained cellulosic material.					
32135259	2	59	theme	lignin	329:334	arg1	removal					318:324	removal	318:324	removal of lignin and hemicelluloses from material	318:367	The procedure involved an alkali treatment and chlorine-free bleaching for removal of lignin and hemicelluloses from material.					
32135259	9	60	theme	cellulosic	1026:1035	arg1	material					1037:1044	the cellulosic material	1022:1044	the cellulosic material	1022:1044	The TGA curve showed the thermal degradation pattern of the cellulosic material and it was closed to the thermal behavior of pure cellulose.					
32135259	10	61	theme	obtained	1171:1178	arg1	material					1191:1198	the obtained cellulosic material	1167:1198	the obtained cellulosic material	1167:1198	Finally, nanocellulose was produced by acid hydrolysis from the obtained cellulosic material.					
32135259	0	62	theme	nanocellulose	69:81	arg1	fabrication					83:93	nanocellulose fabrication	69:93	nanocellulose fabrication	69:93	Environmentally benign extraction of cellulose from dunchi fiber for nanocellulose fabrication.					
32135259	12	63	theme	wide	1463:1466	arg1	range					1468:1472	a wide range	1461:1472	a wide range of applications in composite materials	1461:1511	In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials.					
32135259	6	64	theme	crystallinity	747:759	arg1	degree					737:742	the degree	733:742	the degree of crystallinity	733:759	By using Segal method, the degree of crystallinity was found 66.7%.					
32135259	2	65	dep	treatment	276:284	arg1	an					266:267	an	266:267	an	266:267	The procedure involved an alkali treatment and chlorine-free bleaching for removal of lignin and hemicelluloses from material.					
32135259	9	66	theme	material	1037:1044	arg1	pattern					1011:1017	the thermal degradation pattern	987:1017	the thermal degradation pattern of the cellulosic material	987:1044	The TGA curve showed the thermal degradation pattern of the cellulosic material and it was closed to the thermal behavior of pure cellulose.					
32135259	1	67	theme	nanocellulose	228:240	arg1	preparation					213:223	preparation	213:223	preparation of nanocellulose	213:240	In the current study, cellulose was extracted from the plant dunchi fiber by using an ecofriendly method followed by preparation of nanocellulose.					
32169446	4	0	dep	β-d-Glcp-	676:684	arg1	→4,6					693:696	→4,6	693:696	→4,6	693:696	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	4	0	dep	β-d-Glcp-	676:684	arg1	1→					686:687	1→	686:687	1→	686:687	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	9	1	theme	polysaccharide	1094:1107	arg1	HAW1-2					1109:1114	polysaccharide HAW1-2	1094:1114	polysaccharide HAW1-2	1094:1114	These results imply that polysaccharide HAW1-2 may be useful for human by modulating intestinal bacteria and producing short chain fatty acids.					
32169446	1	2	theme	ethanol	255:261	arg1	extraction					243:252	hot-water extraction	233:252	hot-water extraction	233:252	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	1	2	theme	ethanol	255:261	arg1	precipitation					263:275	ethanol precipitation	255:275	ethanol precipitation	255:275	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	4	3	theme	Methylation	541:551	arg1	analysis					553:560	Methylation analysis	541:560	Methylation analysis	541:560	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	2	4	theme	homogenous	366:375	arg1	polysaccharide					377:390	a homogenous polysaccharide	364:390	a homogenous polysaccharide with a molecular weight (Mw) of 8.94 kDa	364:431	HAW1-2 was proved as a homogenous polysaccharide with a molecular weight (Mw) of 8.94 kDa.					
32169446	0	5	from	Isolation	0:8	arg1	pinnatifida					76:86	pinnatifida	76:86	pinnatifida	76:86	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	0	5	from	Isolation	0:8	arg1	microbiota					115:124	gut microbiota	111:124	gut microbiota	111:124	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	4	6	dep	contained	598:606	arg1	1→					709:710	1→	709:710	1→	709:710	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	1	7	theme	DEAE-cellulose	278:291	arg1	extraction					243:252	hot-water extraction	233:252	hot-water extraction	233:252	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	1	7	theme	DEAE-cellulose	278:291	arg1	exchange					299:306	DEAE-cellulose anion exchange and gel permeation chromatography	278:340	exchange	299:306	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	4	8	theme	-β-d-Galp-	629:638	arg1	-β-d-Glcp-					698:707	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	-β-d-Glcp-	698:707	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	5	9	theme	thetaiotamicron	803:817	arg1	growth					781:786	the growth	777:786	the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp	777:930	Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp.					
32169446	0	10	theme	gut	111:113	arg1	microbiota					115:124	gut microbiota	111:124	gut microbiota	111:124	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	4	11	theme	1→	622:623	arg1	-β-d-Glcp-					698:707	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	-β-d-Glcp-	698:707	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	8	12	theme	acetic	998:1003	arg1	acids					1005:1009	more acetic acids	993:1009	more acetic acids	993:1009	generated more acetic acids and propionic acids while BL only generated acetic acids.					
32169446	3	13	theme	Monosaccharide	434:447	arg1	results					461:467	Monosaccharide composition results	434:467	Monosaccharide composition results	434:467	Monosaccharide composition results indicated that HAW1-2 was composed of arabinose, galactose and glucose.					
32169446	1	14	theme	anion	293:297	arg1	extraction					243:252	hot-water extraction	233:252	hot-water extraction	233:252	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	1	14	theme	anion	293:297	arg1	exchange					299:306	DEAE-cellulose anion exchange and gel permeation chromatography	278:340	exchange	299:306	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	4	15	contain	contained	598:606	arg1	HAW1-2					591:596	HAW1-2	591:596	HAW1-2	591:596	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	4	15	contain	contained	598:606	arg2	-β-d-Glcp-					698:707	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	-β-d-Glcp-	698:707	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	2	16	theme	molecular	399:407	arg1	Mw					417:418	Mw	417:418	Mw	417:418	HAW1-2 was proved as a homogenous polysaccharide with a molecular weight (Mw) of 8.94 kDa.					
32169446	2	16	theme	molecular	399:407	arg1	weight					409:414	a molecular weight	397:414	a molecular weight (Mw) of 8.94 kDa	397:431	HAW1-2 was proved as a homogenous polysaccharide with a molecular weight (Mw) of 8.94 kDa.					
32169446	0	17	theme	structure	14:22	arg1	characterization					24:39	structure characterization	14:39	structure characterization	14:39	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	9	18	theme	intestinal	1154:1163	arg1	bacteria					1165:1172	intestinal bacteria	1154:1172	intestinal bacteria	1154:1172	These results imply that polysaccharide HAW1-2 may be useful for human by modulating intestinal bacteria and producing short chain fatty acids.					
32169446	4	19	theme	-α-L-Araf-	661:670	arg1	-β-d-Glcp-					698:707	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	-β-d-Glcp-	698:707	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	2	20	theme	8.94 kDa	424:431	arg1	Mw					417:418	Mw	417:418	Mw	417:418	HAW1-2 was proved as a homogenous polysaccharide with a molecular weight (Mw) of 8.94 kDa.					
32169446	2	20	theme	8.94 kDa	424:431	arg1	weight					409:414	a molecular weight	397:414	a molecular weight (Mw) of 8.94 kDa	397:431	HAW1-2 was proved as a homogenous polysaccharide with a molecular weight (Mw) of 8.94 kDa.					
32169446	5	21	theme	Bioactivity	713:723	arg1	test					725:728	Bioactivity test	713:728	Bioactivity test	713:728	Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp.					
32169446	1	22	theme	gel	312:314	arg1	extraction					243:252	hot-water extraction	233:252	hot-water extraction	233:252	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	1	22	theme	gel	312:314	arg1	chromatography					327:340	DEAE-cellulose anion exchange and gel permeation chromatography	278:340	chromatography	327:340	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	4	23	theme	1→	640:641	arg1	-β-d-Glcp-					698:707	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	-β-d-Glcp-	698:707	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	3	24	theme	composition	449:459	arg1	results					461:467	Monosaccharide composition results	434:467	Monosaccharide composition results	434:467	Monosaccharide composition results indicated that HAW1-2 was composed of arabinose, galactose and glucose.					
32169446	1	25	theme	Crataegus	208:216	arg1	pinnatifida					218:228	Crataegus pinnatifida	208:228	Crataegus pinnatifida	208:228	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	5	26	with	competition	895:905	arg1	spp					928:930	the Bacteroides spp	912:930	the Bacteroides spp	912:930	Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp.					
32169446	1	27	theme	permeation	316:325	arg1	extraction					243:252	hot-water extraction	233:252	hot-water extraction	233:252	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	1	27	theme	permeation	316:325	arg1	chromatography					327:340	DEAE-cellulose anion exchange and gel permeation chromatography	278:340	chromatography	327:340	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	8	28	theme	propionic	1015:1023	arg1	acids					1025:1029	propionic acids	1015:1029	propionic acids	1015:1029	generated more acetic acids and propionic acids while BL only generated acetic acids.					
32169446	0	29	from	pinnatifida	76:86	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	0	29	from	pinnatifida	76:86	arg1	characterization					24:39	structure characterization	14:39	structure characterization	14:39	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	0	29	from	pinnatifida	76:86	arg1	polysaccharide					46:59	a polysaccharide	44:59	a polysaccharide from Crataegus pinnatifida	44:86	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	0	29	from	pinnatifida	76:86	arg1	bioactivity					96:106	its bioactivity	92:106	its bioactivity on gut microbiota	92:124	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	5	30	theme	Bacteroides	791:801	arg1	ovatus					837:842	Bacteroides ovatus	825:842	Bacteroides ovatus (BO)	825:847	Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp.					
32169446	5	30	theme	Bacteroides	791:801	arg1	longum					869:874	Bifidobacterium longum	853:874	Bifidobacterium longum (BL)	853:879	Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp.					
32169446	5	30	theme	Bacteroides	791:801	arg1	BT					820:821	BT	820:821	BT	820:821	Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp.					
32169446	5	30	theme	Bacteroides	791:801	arg1	thetaiotamicron					803:817	Bacteroides thetaiotamicron	791:817	Bacteroides thetaiotamicron (BT)	791:822	Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp.					
32169446	1	31	theme	pinnatifida	218:228	arg1	fruit					199:203	fruit	199:203	fruit of Crataegus pinnatifida	199:228	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	0	32	theme	polysaccharide	46:59	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	0	32	theme	polysaccharide	46:59	arg1	characterization					24:39	structure characterization	14:39	structure characterization	14:39	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	0	32	theme	polysaccharide	46:59	arg1	bioactivity					96:106	its bioactivity	92:106	its bioactivity on gut microbiota	92:124	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	9	33	theme	short	1188:1192	arg1	acids					1206:1210	short chain fatty acids	1188:1210	short chain fatty acids	1188:1210	These results imply that polysaccharide HAW1-2 may be useful for human by modulating intestinal bacteria and producing short chain fatty acids.					
32169446	2	34	with	polysaccharide	377:390	arg1	Mw					417:418	Mw	417:418	Mw	417:418	HAW1-2 was proved as a homogenous polysaccharide with a molecular weight (Mw) of 8.94 kDa.					
32169446	2	34	with	polysaccharide	377:390	arg1	weight					409:414	a molecular weight	397:414	a molecular weight (Mw) of 8.94 kDa	397:431	HAW1-2 was proved as a homogenous polysaccharide with a molecular weight (Mw) of 8.94 kDa.					
32169446	4	35	theme	1→	654:655	arg1	-β-d-Glcp-					698:707	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	-β-d-Glcp-	698:707	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	4	36	theme	NMR	566:568	arg1	spectrum					570:577	NMR spectrum	566:577	NMR spectrum	566:577	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	4	37	dep	-β-d-Glcp-	698:707	arg1	β-d-Glcp-					676:684	β-d-Glcp-	676:684	β-d-Glcp-(1→ and →4,6)	676:697	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	4	37	dep	-β-d-Glcp-	698:707	arg1	→5					658:659	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	→5	658:659	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	4	37	dep	-β-d-Glcp-	698:707	arg1	→4					626:627	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	→4	626:627	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	4	37	dep	-β-d-Glcp-	698:707	arg1	α-L-Araf-					644:652	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	α-L-Araf-	644:652	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	9	38	theme	chain	1194:1198	arg1	acids					1206:1210	short chain fatty acids	1188:1210	short chain fatty acids	1188:1210	These results imply that polysaccharide HAW1-2 may be useful for human by modulating intestinal bacteria and producing short chain fatty acids.					
32169446	1	39	theme	glucose	129:135	arg1	HAW1-2					161:166	A glucose rich heteroglycan named HAW1-2	127:166	A glucose rich heteroglycan named HAW1-2	127:166	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	9	40	theme	fatty	1200:1204	arg1	acids					1206:1210	short chain fatty acids	1188:1210	short chain fatty acids	1188:1210	These results imply that polysaccharide HAW1-2 may be useful for human by modulating intestinal bacteria and producing short chain fatty acids.					
32169446	1	41	theme	rich	137:140	arg1	HAW1-2					161:166	A glucose rich heteroglycan named HAW1-2	127:166	A glucose rich heteroglycan named HAW1-2	127:166	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	1	42	theme	hot-water	233:241	arg1	extraction					243:252	hot-water extraction	233:252	hot-water extraction	233:252	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	1	42	theme	hot-water	233:241	arg1	precipitation					263:275	ethanol precipitation	255:275	ethanol precipitation	255:275	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	1	42	theme	hot-water	233:241	arg1	exchange					299:306	DEAE-cellulose anion exchange and gel permeation chromatography	278:340	exchange	299:306	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	1	42	theme	hot-water	233:241	arg1	chromatography					327:340	DEAE-cellulose anion exchange and gel permeation chromatography	278:340	chromatography	327:340	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	0	43	from	bioactivity	96:106	arg1	pinnatifida					76:86	pinnatifida	76:86	pinnatifida	76:86	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	0	43	from	bioactivity	96:106	arg1	microbiota					115:124	gut microbiota	111:124	gut microbiota	111:124	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	5	44	theme	Bacteroides	916:926	arg1	spp					928:930	the Bacteroides spp	912:930	the Bacteroides spp	912:930	Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp.					
32169446	1	45	theme	heteroglycan	142:153	arg1	HAW1-2					161:166	A glucose rich heteroglycan named HAW1-2	127:166	A glucose rich heteroglycan named HAW1-2	127:166	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	4	46	theme	-β-d-Glcp-	611:620	arg1	-β-d-Glcp-					698:707	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	-β-d-Glcp-	698:707	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
32169446	8	47	theme	acetic	1055:1060	arg1	acids					1062:1066	acetic acids	1055:1066	acetic acids	1055:1066	generated more acetic acids and propionic acids while BL only generated acetic acids.					
32169446	0	48	from	characterization	24:39	arg1	pinnatifida					76:86	pinnatifida	76:86	pinnatifida	76:86	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	0	48	from	characterization	24:39	arg1	microbiota					115:124	gut microbiota	111:124	gut microbiota	111:124	Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.					
32169446	1	49	theme	named	155:159	arg1	HAW1-2					161:166	A glucose rich heteroglycan named HAW1-2	127:166	A glucose rich heteroglycan named HAW1-2	127:166	A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography.					
32169446	4	50	theme	1→	672:673	arg1	-β-d-Glcp-					698:707	→4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-	608:707	-β-d-Glcp-	698:707	Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→.					
33688648	6	0	theme	S-RBD	1521:1525	arg1	variants					1527:1534	emergent SARS-CoV-2 S-RBD variants	1501:1534	emergent SARS-CoV-2 S-RBD variants	1501:1534	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	5	1	theme	proteoform-resolved	1230:1248	arg1	mapping					1250:1256	a high-resolution proteoform-resolved mapping	1212:1256	a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans	1212:1395	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	1	2	theme	cell	280:283	arg1	entry					285:289	host cell entry	275:289	host cell entry	275:289	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	4	3	theme	phase	854:858	arg1	variants					871:878	their gas phase structural variants	844:878	their gas phase structural variants	844:878	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	2	4	located	found	466:470	arg1	S-RBD					514:518	S-RBD	514:518	S-RBD	514:518	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	2	4	located	found	466:470	arg1	domain					506:511	the S protein regional-binding domain	475:511	the S protein regional-binding domain (S-RBD)	475:519	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	2	4	located	found	466:470	arg2	O-glycans					456:464	the new O-glycans	448:464	the new O-glycans found on the S protein regional-binding domain (S-RBD)	448:519	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	4	5	theme	resonance	939:947	arg1	analysis					960:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	4	6	dep	analysis	960:967	arg1	transform					915:923	transform	915:923	transform	915:923	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	6	7	from	heterogeneity	1484:1496	arg1	general					1573:1579	general	1573:1579	general	1573:1579	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	1	8	theme	glycosylated	225:236	arg1	protein					256:262	an extensively glycosylated surface spike (S) protein	210:262	an extensively glycosylated surface spike (S) protein	210:262	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	4	9	theme	FTICR	950:954	arg1	analysis					960:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	6	10	gly	O-glycoproteins	1554:1568	arg1	O-glycoproteins					1554:1568	other O-glycoproteins	1548:1568	other O-glycoproteins	1548:1568	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	1	11	theme	spike	246:250	arg1	protein					256:262	an extensively glycosylated surface spike (S) protein	210:262	an extensively glycosylated surface spike (S) protein	210:262	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	4	12	theme	Fourier	907:913	arg1	analysis					960:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	2	13	theme	glycoform	572:580	arg1	analysis					582:589	intact glycoform analysis	565:589	intact glycoform analysis	565:589	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	1	14	theme	Severe	140:145	arg1	coronavirus					174:184	Severe acute respiratory syndrome coronavirus 2	140:186	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	140:199	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	1	14	theme	Severe	140:145	arg1	SARS-CoV-2					189:198	SARS-CoV-2	189:198	SARS-CoV-2	189:198	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	4	15	theme	ion	925:927	arg1	analysis					960:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	4	16	theme	trapped	739:745	arg1	spectrometry					760:771	trapped ion mobility spectrometry	739:771	trapped ion mobility spectrometry (TIMS)	739:778	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	4	16	theme	trapped	739:745	arg1	TIMS					774:777	TIMS	774:777	TIMS	774:777	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	1	17	theme	respiratory	153:163	arg1	coronavirus					174:184	Severe acute respiratory syndrome coronavirus 2	140:186	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	140:199	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	1	17	theme	respiratory	153:163	arg1	SARS-CoV-2					189:198	SARS-CoV-2	189:198	SARS-CoV-2	189:198	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	5	18	theme	molecular	1325:1333	arg1	foundation					1335:1344	a strong molecular foundation	1316:1344	a strong molecular foundation to uncover the functional roles of their O-glycans	1316:1395	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	0	19	theme	Receptor-Binding	69:84	arg1	Domain					86:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	3	20	theme	complete	612:619	arg1	characterization					632:647	the complete structural characterization	608:647	the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS)	608:723	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	5	21	theme	glycoprotein	1291:1302	arg1	mapping					1250:1256	a high-resolution proteoform-resolved mapping	1212:1256	a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans	1212:1395	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	4	22	theme	MS	957:958	arg1	analysis					960:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	0	23	theme	Native	105:110	arg1	Spectrometry					126:137	Native Top-Down Mass Spectrometry	105:137	Native Top-Down Mass Spectrometry	105:137	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	4	24	gly	O-glycoforms	974:985	arg1	S-RBD					994:998	the S-RBD	990:998	the S-RBD	990:998	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	0	25	theme	Mass	121:124	arg1	Spectrometry					126:137	Native Top-Down Mass Spectrometry	105:137	Native Top-Down Mass Spectrometry	105:137	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	0	26	theme	SARS-CoV-2	44:53	arg1	Domain					86:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	4	27	theme	mobility	751:758	arg1	spectrometry					760:771	trapped ion mobility spectrometry	739:771	trapped ion mobility spectrometry (TIMS)	739:778	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	4	27	theme	mobility	751:758	arg1	TIMS					774:777	TIMS	774:777	TIMS	774:777	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	0	28	theme	Structural	0:9	arg1	Heterogeneity					23:35	Structural O-Glycoform Heterogeneity	0:35	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain	0:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	2	29	theme	new	452:454	arg1	O-glycans					456:464	the new O-glycans	448:464	the new O-glycans found on the S protein regional-binding domain (S-RBD)	448:519	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	6	30	theme	O-glycoform	1472:1482	arg1	heterogeneity					1484:1496	the structural O-glycoform heterogeneity	1457:1496	the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general	1457:1579	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	6	31	gly	heterogeneity	1484:1496	arg1	variants					1527:1534	emergent SARS-CoV-2 S-RBD variants	1501:1534	emergent SARS-CoV-2 S-RBD variants	1501:1534	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	6	31	gly	heterogeneity	1484:1496	arg1	O-glycoproteins					1554:1568	other O-glycoproteins	1548:1568	other O-glycoproteins	1548:1568	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	2	32	theme	protein	481:487	arg1	S-RBD					514:518	S-RBD	514:518	S-RBD	514:518	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	2	32	theme	protein	481:487	arg1	domain					506:511	the S protein regional-binding domain	475:511	the S protein regional-binding domain (S-RBD)	475:519	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	3	33	theme	proteoforms	668:678	arg1	characterization					632:647	the complete structural characterization	608:647	the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS)	608:723	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	2	34	theme	relative	426:433	arg1	abundance					435:443	relative abundance	426:443	relative abundance	426:443	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	1	35	theme	protein	301:307	arg1	glycosylation					309:321	the S protein glycosylation	295:321	the S protein glycosylation	295:321	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	4	36	theme	S-RBD	994:998	arg1	O-glycoforms					974:985	the O-glycoforms	970:985	the O-glycoforms of the S-RBD	970:998	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	3	37	theme	intact	652:657	arg1	proteoforms					668:678	intact O-glycan proteoforms	652:678	intact O-glycan proteoforms using native top-down mass spectrometry (MS)	652:723	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	0	38	theme	Spike	55:59	arg1	Domain					86:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	5	39	theme	native	1181:1186	arg1	MS					1197:1198	native top-down MS	1181:1198	native top-down MS	1181:1198	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	3	40	theme	mass	702:705	arg1	MS					721:722	MS	721:722	MS	721:722	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	3	40	theme	mass	702:705	arg1	spectrometry					707:718	native top-down mass spectrometry	686:718	native top-down mass spectrometry (MS)	686:723	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	5	41	theme	diverse	1261:1267	arg1	O-glycoforms					1269:1280	diverse O-glycoforms	1261:1280	diverse O-glycoforms	1261:1280	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	3	42	theme	native	686:691	arg1	MS					721:722	MS	721:722	MS	721:722	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	3	42	theme	native	686:691	arg1	spectrometry					707:718	native top-down mass spectrometry	686:718	native top-down mass spectrometry (MS)	686:723	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	4	43	theme	protein	804:810	arg1	conformers					812:821	the protein conformers	800:821	the protein conformers of S-RBD	800:830	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	6	44	theme	variants	1527:1534	arg1	heterogeneity					1484:1496	the structural O-glycoform heterogeneity	1457:1496	the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general	1457:1579	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	5	45	theme	high-resolution	1214:1228	arg1	mapping					1250:1256	a high-resolution proteoform-resolved mapping	1212:1256	a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans	1212:1395	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	4	46	theme	structural	860:869	arg1	variants					871:878	their gas phase structural variants	844:878	their gas phase structural variants	844:878	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	4	47	theme	relative	1072:1079	arg1	abundance					1091:1099	their relative molecular abundance	1066:1099	their relative molecular abundance	1066:1099	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	6	48	theme	SARS-CoV-2	1510:1519	arg1	variants					1527:1534	emergent SARS-CoV-2 S-RBD variants	1501:1534	emergent SARS-CoV-2 S-RBD variants	1501:1534	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	1	49	theme	host	275:278	arg1	entry					285:289	host cell entry	275:289	host cell entry	275:289	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	1	50	gly	glycosylated	225:236	arg1	protein					256:262	an extensively glycosylated surface spike (S) protein	210:262	an extensively glycosylated surface spike (S) protein	210:262	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	4	51	theme	gas	850:852	arg1	variants					871:878	their gas phase structural variants	844:878	their gas phase structural variants	844:878	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	3	52	theme	top-down	693:700	arg1	MS					721:722	MS	721:722	MS	721:722	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	3	52	theme	top-down	693:700	arg1	spectrometry					707:718	native top-down mass spectrometry	686:718	native top-down mass spectrometry (MS)	686:723	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	4	53	theme	cyclotron	929:937	arg1	analysis					960:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	4	54	theme	first	1137:1141	arg1	time					1143:1146	the first time	1133:1146	the first time	1133:1146	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	6	55	theme	O-glycoproteins	1554:1568	arg1	heterogeneity					1484:1496	the structural O-glycoform heterogeneity	1457:1496	the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general	1457:1579	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	1	56	theme	surface	238:244	arg1	protein					256:262	an extensively glycosylated surface spike (S) protein	210:262	an extensively glycosylated surface spike (S) protein	210:262	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	6	57	theme	proteoform-resolved	1403:1421	arg1	approach					1423:1430	This proteoform-resolved approach	1398:1430	This proteoform-resolved approach	1398:1430	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	4	58	theme	ultrahigh-resolution	886:905	arg1	analysis					960:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis	886:967	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	3	59	theme	structural	621:630	arg1	characterization					632:647	the complete structural characterization	608:647	the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS)	608:723	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	1	60	theme	acute	147:151	arg1	coronavirus					174:184	Severe acute respiratory syndrome coronavirus 2	140:186	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	140:199	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	1	60	theme	acute	147:151	arg1	SARS-CoV-2					189:198	SARS-CoV-2	189:198	SARS-CoV-2	189:198	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	2	61	theme	intact	565:570	arg1	analysis					582:589	intact glycoform analysis	565:589	intact glycoform analysis	565:589	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	5	62	theme	strong	1318:1323	arg1	foundation					1335:1344	a strong molecular foundation	1316:1344	a strong molecular foundation to uncover the functional roles of their O-glycans	1316:1395	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	0	63	theme	Domain	86:91	arg1	Heterogeneity					23:35	Structural O-Glycoform Heterogeneity	0:35	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain	0:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	1	64	theme	syndrome	165:172	arg1	coronavirus					174:184	Severe acute respiratory syndrome coronavirus 2	140:186	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	140:199	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	1	64	theme	syndrome	165:172	arg1	SARS-CoV-2					189:198	SARS-CoV-2	189:198	SARS-CoV-2	189:198	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	5	65	theme	S	1289:1289	arg1	glycoprotein					1291:1302	the S glycoprotein	1285:1302	the S glycoprotein	1285:1302	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	2	66	theme	regional-binding	489:504	arg1	S-RBD					514:518	S-RBD	514:518	S-RBD	514:518	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	2	66	theme	regional-binding	489:504	arg1	domain					506:511	the S protein regional-binding domain	475:511	the S protein regional-binding domain (S-RBD)	475:519	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	0	67	theme	Top-Down	112:119	arg1	Spectrometry					126:137	Native Top-Down Mass Spectrometry	105:137	Native Top-Down Mass Spectrometry	105:137	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	1	68	theme	viral	358:362	arg1	binding/function					364:379	viral binding/function	358:379	viral binding/function	358:379	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	5	69	theme	functional	1361:1370	arg1	roles					1372:1376	the functional roles	1357:1376	the functional roles of their O-glycans	1357:1395	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	1	70	theme	S	253:253	arg1	protein					256:262	an extensively glycosylated surface spike (S) protein	210:262	an extensively glycosylated surface spike (S) protein	210:262	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	0	71	theme	O-Glycoform	11:21	arg1	Heterogeneity					23:35	Structural O-Glycoform Heterogeneity	0:35	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain	0:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	2	72	theme	O-glycans	456:464	arg1	structures					411:420	structures	411:420	structures	411:420	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	2	72	theme	O-glycans	456:464	arg1	abundance					435:443	relative abundance	426:443	relative abundance	426:443	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	4	73	theme	ion	747:749	arg1	spectrometry					760:771	trapped ion mobility spectrometry	739:771	trapped ion mobility spectrometry (TIMS)	739:778	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	4	73	theme	ion	747:749	arg1	TIMS					774:777	TIMS	774:777	TIMS	774:777	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	2	74	dep	structures	411:420	arg1	the					407:409	the	407:409	the	407:409	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	3	75	theme	O-glycan	659:666	arg1	proteoforms					668:678	intact O-glycan proteoforms	652:678	intact O-glycan proteoforms using native top-down mass spectrometry (MS)	652:723	Here, we report the complete structural characterization of intact O-glycan proteoforms using native top-down mass spectrometry (MS).					
33688648	6	76	theme	structural	1461:1470	arg1	heterogeneity					1484:1496	the structural O-glycoform heterogeneity	1457:1496	the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general	1457:1579	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	1	77	theme	S	299:299	arg1	glycosylation					309:321	the S protein glycosylation	295:321	the S protein glycosylation	295:321	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation is strongly implicated in altering viral binding/function and infectivity.					
33688648	2	78	theme	S	479:479	arg1	S-RBD					514:518	S-RBD	514:518	S-RBD	514:518	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	2	78	theme	S	479:479	arg1	domain					506:511	the S protein regional-binding domain	475:511	the S protein regional-binding domain (S-RBD)	475:519	However, the structures and relative abundance of the new O-glycans found on the S protein regional-binding domain (S-RBD) remain cryptic because of the challenges in intact glycoform analysis.					
33688648	0	79	theme	Protein	61:67	arg1	Domain					86:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	0	80	gly	Heterogeneity	23:35	arg1	Domain					86:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	the SARS-CoV-2 Spike Protein Receptor-Binding Domain	40:91	Structural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Native Top-Down Mass Spectrometry.					
33688648	5	81	gly	glycoprotein	1291:1302	arg1	glycoprotein					1291:1302	the S glycoprotein	1285:1302	the S glycoprotein	1285:1302	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	4	82	theme	S-RBD	826:830	arg1	conformers					812:821	the protein conformers	800:821	the protein conformers of S-RBD	800:830	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	6	83	theme	other	1548:1552	arg1	O-glycoproteins					1554:1568	other O-glycoproteins	1548:1568	other O-glycoproteins	1548:1568	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
33688648	5	84	theme	top-down	1188:1195	arg1	MS					1197:1198	native top-down MS	1181:1198	native top-down MS	1181:1198	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	4	85	theme	molecular	1081:1089	arg1	abundance					1091:1099	their relative molecular abundance	1066:1099	their relative molecular abundance	1066:1099	By combining trapped ion mobility spectrometry (TIMS), which can separate the protein conformers of S-RBD and analyze their gas phase structural variants, with ultrahigh-resolution Fourier transform ion cyclotron resonance (FTICR) MS analysis, the O-glycoforms of the S-RBD are comprehensively characterized, so that seven O-glycoforms and their relative molecular abundance are structurally elucidated for the first time.					
33688648	5	86	theme	O-glycans	1387:1395	arg1	roles					1372:1376	the functional roles	1357:1376	the functional roles of their O-glycans	1357:1395	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	5	87	theme	O-glycoforms	1269:1280	arg1	mapping					1250:1256	a high-resolution proteoform-resolved mapping	1212:1256	a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans	1212:1395	These findings demonstrate that native top-down MS can provide a high-resolution proteoform-resolved mapping of diverse O-glycoforms of the S glycoprotein, which lays a strong molecular foundation to uncover the functional roles of their O-glycans.					
33688648	6	88	theme	emergent	1501:1508	arg1	variants					1527:1534	emergent SARS-CoV-2 S-RBD variants	1501:1534	emergent SARS-CoV-2 S-RBD variants	1501:1534	This proteoform-resolved approach can be applied to reveal the structural O-glycoform heterogeneity of emergent SARS-CoV-2 S-RBD variants, as well as other O-glycoproteins in general.					
34687018	0	0	from	Glycosylation	17:29	arg1	Diseases					34:41	Diseases	34:41	Diseases	34:41	Immunoglobulin G Glycosylation in Diseases.					
34687018	4	1	theme	several	863:869	arg1	diseases					926:933	several infectious and allo- and autoimmune antibody-dependent diseases	863:933	several infectious and allo- and autoimmune antibody-dependent diseases	863:933	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	3	2	from	pattern	488:494	arg1	patients					499:506	patients	499:506	patients	499:506	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	4	3	from	role	834:837	arg1	pathology					850:858	disease pathology	842:858	disease pathology	842:858	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	4	4	theme	direct	827:832	arg1	role					834:837	a direct role	825:837	a direct role in disease pathology	825:858	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	1	5	theme	auto-	138:142	arg1	diseases					210:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	4	6	gly	glycosylation	764:776	arg1	IgG					806:808	antigen-specific IgG	789:808	antigen-specific IgG	789:808	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	4	7	theme	disease	842:848	arg1	pathology					850:858	disease pathology	842:858	disease pathology	842:858	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	3	8	theme	changed	462:468	arg1	pattern					488:494	a changed IgG glycosylation pattern	460:494	a changed IgG glycosylation pattern in patients	460:506	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	1	9	theme	immunoglobulin	55:68	arg1	IgG					73:75	IgG	73:75	IgG	73:75	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	1	9	theme	immunoglobulin	55:68	arg1	G					70:70	immunoglobulin G	55:70	immunoglobulin G (IgG) glycosylation pattern	55:98	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	5	10	theme	given	970:974	arg1	disease					976:982	any given disease	966:982	any given disease	966:982	Depending on the specifics of any given disease, IgG glycosylation read-out might therefore in the future be developed into a useful clinical biomarker or a supplementary to currently used biomarkers.					
34687018	4	11	theme	antibody-dependent	907:924	arg1	diseases					926:933	several infectious and allo- and autoimmune antibody-dependent diseases	863:933	several infectious and allo- and autoimmune antibody-dependent diseases	863:933	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	5	12	theme	useful	1062:1067	arg1	biomarker					1078:1086	a useful clinical biomarker	1060:1086	a useful clinical biomarker	1060:1086	Depending on the specifics of any given disease, IgG glycosylation read-out might therefore in the future be developed into a useful clinical biomarker or a supplementary to currently used biomarkers.					
34687018	1	13	theme	G	70:70	arg1	pattern					92:98	immunoglobulin G (IgG) glycosylation pattern	55:98	immunoglobulin G (IgG) glycosylation pattern	55:98	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	1	14	from	Changes	44:50	arg1	pattern					92:98	immunoglobulin G (IgG) glycosylation pattern	55:98	immunoglobulin G (IgG) glycosylation pattern	55:98	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	2	15	contain	contains	233:240	arg1	chapter					225:231	This chapter	220:231	This chapter	220:231	This chapter contains an updated catalog of over 140 studies within which IgG glycosylation analysis was performed in a disease setting.					
34687018	2	15	contain	contains	233:240	arg2	catalog					253:259	an updated catalog	242:259	an updated catalog of over 140 studies within which IgG glycosylation analysis was performed in a disease setting	242:354	This chapter contains an updated catalog of over 140 studies within which IgG glycosylation analysis was performed in a disease setting.					
34687018	5	16	theme	clinical	1069:1076	arg1	biomarker					1078:1086	a useful clinical biomarker	1060:1086	a useful clinical biomarker	1060:1086	Depending on the specifics of any given disease, IgG glycosylation read-out might therefore in the future be developed into a useful clinical biomarker or a supplementary to currently used biomarkers.					
34687018	2	17	theme	updated	245:251	arg1	catalog					253:259	an updated catalog	242:259	an updated catalog of over 140 studies within which IgG glycosylation analysis was performed in a disease setting	242:354	This chapter contains an updated catalog of over 140 studies within which IgG glycosylation analysis was performed in a disease setting.					
34687018	3	18	from	effector	616:623	arg1	pathology					636:644	disease pathology	628:644	disease pathology	628:644	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	4	19	theme	IgG	806:808	arg1	profile					778:784	the glycosylation profile	760:784	the glycosylation profile of antigen-specific IgG	760:808	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	1	20	theme	alloimmune	148:157	arg1	diseases					210:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	0	21	theme	G	15:15	arg1	Glycosylation					17:29	Immunoglobulin G Glycosylation	0:29	Immunoglobulin G Glycosylation in Diseases	0:41	Immunoglobulin G Glycosylation in Diseases.					
34687018	3	22	theme	glycans	386:392	arg1	composition					367:377	the composition	363:377	the composition of IgG glycans	363:392	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	0	23	theme	Immunoglobulin	0:13	arg1	Glycosylation					17:29	Immunoglobulin G Glycosylation	0:29	Immunoglobulin G Glycosylation in Diseases	0:41	Immunoglobulin G Glycosylation in Diseases.					
34687018	2	24	theme	studies	273:279	arg1	catalog					253:259	an updated catalog	242:259	an updated catalog of over 140 studies within which IgG glycosylation analysis was performed in a disease setting	242:354	This chapter contains an updated catalog of over 140 studies within which IgG glycosylation analysis was performed in a disease setting.					
34687018	2	25	theme	disease	340:346	arg1	setting					348:354	a disease setting	338:354	a disease setting	338:354	This chapter contains an updated catalog of over 140 studies within which IgG glycosylation analysis was performed in a disease setting.					
34687018	4	26	theme	IgG	693:695	arg1	glycopattern					666:677	the glycopattern	662:677	the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background	662:757	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	4	27	theme	allo-	886:890	arg1	diseases					926:933	several infectious and allo- and autoimmune antibody-dependent diseases	863:933	several infectious and allo- and autoimmune antibody-dependent diseases	863:933	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	4	28	theme	antigen-specific	789:804	arg1	IgG					806:808	antigen-specific IgG	789:808	antigen-specific IgG	789:808	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	3	29	theme	effector	419:426	arg1	functions					428:436	its effector functions	415:436	its effector functions	415:436	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	1	30	theme	glycosylation	78:90	arg1	pattern					92:98	immunoglobulin G (IgG) glycosylation pattern	55:98	immunoglobulin G (IgG) glycosylation pattern	55:98	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	3	31	theme	glycosylation	474:486	arg1	pattern					488:494	a changed IgG glycosylation pattern	460:494	a changed IgG glycosylation pattern in patients	460:506	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	3	32	theme	functional	605:614	arg1	effector					616:623	a functional effector	603:623	a functional effector in disease pathology	603:644	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	1	33	theme	cardiometabolic	172:186	arg1	diseases					210:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	4	34	theme	autoimmune	896:905	arg1	diseases					926:933	several infectious and allo- and autoimmune antibody-dependent diseases	863:933	several infectious and allo- and autoimmune antibody-dependent diseases	863:933	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	5	35	theme	IgG	985:987	arg1	read-out					1003:1010	IgG glycosylation read-out	985:1010	IgG glycosylation read-out	985:1010	Depending on the specifics of any given disease, IgG glycosylation read-out might therefore in the future be developed into a useful clinical biomarker or a supplementary to currently used biomarkers.					
34687018	1	36	theme	infectious	160:169	arg1	diseases					210:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	3	37	theme	IgG	382:384	arg1	glycans					386:392	IgG glycans	382:392	IgG glycans	382:392	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	5	38	theme	used	1120:1123	arg1	biomarkers					1125:1134	currently used biomarkers	1110:1134	currently used biomarkers	1110:1134	Depending on the specifics of any given disease, IgG glycosylation read-out might therefore in the future be developed into a useful clinical biomarker or a supplementary to currently used biomarkers.					
34687018	4	39	theme	glycosylation	764:776	arg1	profile					778:784	the glycosylation profile	760:784	the glycosylation profile of antigen-specific IgG	760:808	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	1	40	theme	malignant	189:197	arg1	diseases					210:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	3	41	theme	disease	529:535	arg1	development					537:547	disease development	529:547	disease development	529:547	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	2	42	theme	glycosylation	298:310	arg1	analysis					312:319	IgG glycosylation analysis	294:319	IgG glycosylation analysis	294:319	This chapter contains an updated catalog of over 140 studies within which IgG glycosylation analysis was performed in a disease setting.					
34687018	4	43	theme	infectious	871:880	arg1	diseases					926:933	several infectious and allo- and autoimmune antibody-dependent diseases	863:933	several infectious and allo- and autoimmune antibody-dependent diseases	863:933	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	5	44	theme	glycosylation	989:1001	arg1	read-out					1003:1010	IgG glycosylation read-out	985:1010	IgG glycosylation read-out	985:1010	Depending on the specifics of any given disease, IgG glycosylation read-out might therefore in the future be developed into a useful clinical biomarker or a supplementary to currently used biomarkers.					
34687018	2	45	theme	IgG	294:296	arg1	analysis					312:319	IgG glycosylation analysis	294:319	IgG glycosylation analysis	294:319	This chapter contains an updated catalog of over 140 studies within which IgG glycosylation analysis was performed in a disease setting.					
34687018	4	46	theme	systemic	726:733	arg1	background					748:757	the systemic inflammatory background	722:757	the systemic inflammatory background	722:757	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	4	47	theme	inflammatory	735:746	arg1	background					748:757	the systemic inflammatory background	722:757	the systemic inflammatory background	722:757	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	4	48	theme	bulk	682:685	arg1	IgG					693:695	bulk serum IgG	682:695	bulk serum IgG	682:695	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	4	49	theme	serum	687:691	arg1	IgG					693:695	bulk serum IgG	682:695	bulk serum IgG	682:695	In contrast to the glycopattern of bulk serum IgG, which likely relates to the systemic inflammatory background, the glycosylation profile of antigen-specific IgG probably plays a direct role in disease pathology in several infectious and allo- and autoimmune antibody-dependent diseases.					
34687018	3	50	theme	disease	628:634	arg1	pathology					636:644	disease pathology	628:644	disease pathology	628:644	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	5	51	theme	disease	976:982	arg1	specifics					953:961	the specifics	949:961	the specifics of any given disease	949:982	Depending on the specifics of any given disease, IgG glycosylation read-out might therefore in the future be developed into a useful clinical biomarker or a supplementary to currently used biomarkers.					
34687018	1	52	theme	vast	124:127	arg1	array					129:133	a vast array	122:133	a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	122:217	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	1	53	theme	other	204:208	arg1	diseases					210:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	138:217	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	1	54	located	observed	110:117	arg1	array					129:133	a vast array	122:133	a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	122:217	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	1	54	located	observed	110:117	arg2	Changes					44:50	Changes	44:50	Changes in immunoglobulin G (IgG) glycosylation pattern	44:98	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
34687018	3	55	theme	IgG	470:472	arg1	pattern					488:494	a changed IgG glycosylation pattern	460:494	a changed IgG glycosylation pattern in patients	460:506	Since the composition of IgG glycans is known to modulate its effector functions, it is suggested that a changed IgG glycosylation pattern in patients might be involved in disease development and progression, representing a predisposition and/or a functional effector in disease pathology.					
34687018	1	56	theme	diseases	210:217	arg1	array					129:133	a vast array	122:133	a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases	122:217	Changes in immunoglobulin G (IgG) glycosylation pattern have been observed in a vast array of auto- and alloimmune, infectious, cardiometabolic, malignant, and other diseases.					
32088239	2	0	theme	polysaccharides	484:498	arg1	profiles					418:425	the resulting integrative profiles	392:425	the resulting integrative profiles	392:425	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	0	theme	polysaccharides	484:498	arg1	structural-fingerprinting					455:479	structural-fingerprinting	455:479	structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF)	455:683	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	1	1	theme	gas-liquid	159:168	arg1	chromatography					170:183	a sequential gas-liquid chromatography	146:183	a sequential gas-liquid chromatography	146:183	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	4	2	theme	heatmap	971:977	arg1	analysis					979:986	heatmap analysis	971:986	heatmap analysis	971:986	Similarity analysis, heatmap analysis and principal component analysis were further performed to discern three Panax species based on SNF data sets.					
32088239	0	3	theme	gas-liquid	82:91	arg1	chromatography					93:106	gas-liquid chromatography	82:106	gas-liquid chromatography	82:106	Structural-fingerprinting of polysaccharides to discern Panax species by means of gas-liquid chromatography and mass spectrometry.					
32088239	2	4	with	polysaccharides	484:498	arg1	MCF					556:558	MCF	556:558	MCF	556:558	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	4	with	polysaccharides	484:498	arg1	OCF					680:682	OCF	680:682	OCF	680:682	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	4	with	polysaccharides	484:498	arg1	fingerprinting					540:553	monosaccharide compositional fingerprinting	511:553	monosaccharide compositional fingerprinting (MCF)	511:559	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	4	with	polysaccharides	484:498	arg1	fingerprinting					664:677	oligosaccharide compositional fingerprinting	634:677	oligosaccharide compositional fingerprinting (OCF)	634:683	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	4	with	polysaccharides	484:498	arg1	degradation					568:578	Smith degradation	562:578	Smith degradation	562:578	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	1	5	theme	chromatography	170:183	arg1	route					207:211	a sequential gas-liquid chromatography and mass spectrometry route	146:211	a sequential gas-liquid chromatography and mass spectrometry route	146:211	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	4	6	theme	principal	992:1000	arg1	analysis					1012:1019	principal component analysis	992:1019	principal component analysis	992:1019	Similarity analysis, heatmap analysis and principal component analysis were further performed to discern three Panax species based on SNF data sets.					
32088239	3	7	theme	PG	812:813	arg1	similarity					798:807	the high interspecific similarity	775:807	the high interspecific similarity of PG and PQ	775:820	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	0	8	theme	mass	112:115	arg1	spectrometry					117:128	mass spectrometry	112:128	mass spectrometry	112:128	Structural-fingerprinting of polysaccharides to discern Panax species by means of gas-liquid chromatography and mass spectrometry.					
32088239	4	9	theme	SNF	1084:1086	arg1	sets					1093:1096	SNF data sets	1084:1096	SNF data sets	1084:1096	Similarity analysis, heatmap analysis and principal component analysis were further performed to discern three Panax species based on SNF data sets.					
32088239	5	10	theme	structural	1173:1182	arg1	backbones					1184:1192	the common structural backbones	1162:1192	the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates	1162:1343	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	10	theme	structural	1173:1182	arg1	1 → structures					1120:1133	1 → structures	1120:1133	1 → structures	1120:1133	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	10	theme	structural	1173:1182	arg1	-Hexp-					1113:1118	The linear →4)-Hexp-	1099:1118	The linear →4)-Hexp-	1099:1118	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	2	11	theme	compositional	650:662	arg1	OCF					680:682	OCF	680:682	OCF	680:682	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	11	theme	compositional	650:662	arg1	fingerprinting					664:677	oligosaccharide compositional fingerprinting	634:677	oligosaccharide compositional fingerprinting (OCF)	634:683	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	5	12	from	backbones	1184:1192	arg1	chains					1202:1207	side chains	1197:1207	side chains	1197:1207	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	12	from	backbones	1184:1192	arg1	regions					1219:1225	smooth regions	1212:1225	smooth regions	1212:1225	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	3	13	theme	interspecific	784:796	arg1	similarity					798:807	the high interspecific similarity	775:807	the high interspecific similarity of PG and PQ	775:820	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	2	14	theme	monosaccharide	511:524	arg1	MCF					556:558	MCF	556:558	MCF	556:558	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	14	theme	monosaccharide	511:524	arg1	fingerprinting					540:553	monosaccharide compositional fingerprinting	511:553	monosaccharide compositional fingerprinting (MCF)	511:559	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	15	theme	non-degradation	584:598	arg1	fingerprinting					600:613	non-degradation fingerprinting	584:613	non-degradation fingerprinting	584:613	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	16	theme	oligosaccharide	634:648	arg1	OCF					680:682	OCF	680:682	OCF	680:682	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	16	theme	oligosaccharide	634:648	arg1	fingerprinting					664:677	oligosaccharide compositional fingerprinting	634:677	oligosaccharide compositional fingerprinting (OCF)	634:683	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	5	17	theme	chain	1239:1243	arg1	chains					1202:1207	side chains	1197:1207	side chains	1197:1207	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	17	theme	chain	1239:1243	arg1	regions					1219:1225	smooth regions	1212:1225	smooth regions	1212:1225	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	6	18	theme	polysaccharides	1520:1534	arg1	analysis					1502:1509	analysis	1502:1509	analysis of plant polysaccharides	1502:1534	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	3	19	theme	ratio	932:936	arg1	absence					899:905	absence	899:905	absence	899:905	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	3	19	theme	ratio	932:936	arg1	presence					887:894	the presence	883:894	the presence	883:894	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	4	20	theme	Panax	1061:1065	arg1	species					1067:1073	three Panax species	1055:1073	three Panax species based on SNF data sets	1055:1096	Similarity analysis, heatmap analysis and principal component analysis were further performed to discern three Panax species based on SNF data sets.					
32088239	2	21	theme	stepped	362:368	arg1	parameters					380:389	stepped structure parameters	362:389	stepped structure parameters	362:389	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	3	22	theme	high	779:782	arg1	similarity					798:807	the high interspecific similarity	775:807	the high interspecific similarity of PG and PQ	775:820	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	6	23	theme	generalization	1472:1485	arg1	capability					1487:1496	satisfactory generalization capability	1459:1496	high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides	1425:1534	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	1	24	theme	mass	189:192	arg1	spectrometry					194:205	mass spectrometry	189:205	mass spectrometry	189:205	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	4	25	theme	Similarity	950:959	arg1	analysis					961:968	Similarity analysis	950:968	Similarity analysis	950:968	Similarity analysis, heatmap analysis and principal component analysis were further performed to discern three Panax species based on SNF data sets.					
32088239	2	26	dep	degradation	568:578	arg1	SNF					624:626	SNF	624:626	SNF	624:626	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	26	dep	degradation	568:578	arg1	SDF					616:618	SDF	616:618	SDF	616:618	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	5	27	theme	partial	1319:1325	arg1	hydrolyzates					1332:1343	partial acid hydrolyzates	1319:1343	partial acid hydrolyzates	1319:1343	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	0	28	dep	chromatography	93:106	arg1	means					73:77	means	73:77	means	73:77	Structural-fingerprinting of polysaccharides to discern Panax species by means of gas-liquid chromatography and mass spectrometry.					
32088239	6	29	theme	satisfactory	1459:1470	arg1	capability					1487:1496	satisfactory generalization capability	1459:1496	high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides	1425:1534	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	1	30	theme	spectrometry	194:205	arg1	route					207:211	a sequential gas-liquid chromatography and mass spectrometry route	146:211	a sequential gas-liquid chromatography and mass spectrometry route	146:211	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	6	31	contain	possesses	1415:1423	arg1	approach					1406:1413	the established SFP approach	1386:1413	the established SFP approach	1386:1413	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	6	31	contain	possesses	1415:1423	arg2	capability					1487:1496	satisfactory generalization capability	1459:1496	high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides	1425:1534	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	6	31	contain	possesses	1415:1423	arg2	comprehensibility					1430:1446	high comprehensibility	1425:1446	high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides	1425:1534	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	3	32	theme	visual	725:730	arg1	discrimination					732:745	visual discrimination	725:745	visual discrimination of the three species due to the high interspecific similarity of PG and PQ	725:820	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	5	33	theme	acid	1327:1330	arg1	hydrolyzates					1332:1343	partial acid hydrolyzates	1319:1343	partial acid hydrolyzates	1319:1343	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	3	34	theme	species	760:766	arg1	discrimination					732:745	visual discrimination	725:745	visual discrimination of the three species due to the high interspecific similarity of PG and PQ	725:820	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	6	35	theme	plant	1514:1518	arg1	polysaccharides					1520:1534	plant polysaccharides	1514:1534	plant polysaccharides	1514:1534	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	2	36	theme	integrative	406:416	arg1	profiles					418:425	the resulting integrative profiles	392:425	the resulting integrative profiles	392:425	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	36	theme	integrative	406:416	arg1	structural-fingerprinting					455:479	structural-fingerprinting	455:479	structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF)	455:683	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	5	37	theme	linear	1103:1108	arg1	backbones					1184:1192	the common structural backbones	1162:1192	the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates	1162:1343	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	37	theme	linear	1103:1108	arg1	1 → structures					1120:1133	1 → structures	1120:1133	1 → structures	1120:1133	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	37	theme	linear	1103:1108	arg1	-Hexp-					1113:1118	The linear →4)-Hexp-	1099:1118	The linear →4)-Hexp-	1099:1118	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	38	theme	hydrolyzates	1332:1343	arg1	characterization					1299:1314	characterization	1299:1314	characterization of partial acid hydrolyzates	1299:1343	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	0	39	theme	polysaccharides	29:43	arg1	Structural-fingerprinting					0:24	Structural-fingerprinting	0:24	Structural-fingerprinting of polysaccharides	0:43	Structural-fingerprinting of polysaccharides to discern Panax species by means of gas-liquid chromatography and mass spectrometry.					
32088239	5	40	theme	→4	1110:1111	arg1	backbones					1184:1192	the common structural backbones	1162:1192	the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates	1162:1343	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	40	theme	→4	1110:1111	arg1	1 → structures					1120:1133	1 → structures	1120:1133	1 → structures	1120:1133	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	40	theme	→4	1110:1111	arg1	-Hexp-					1113:1118	The linear →4)-Hexp-	1099:1118	The linear →4)-Hexp-	1099:1118	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	41	from	chains	1202:1207	arg1	PPN					1253:1255	PPN	1253:1255	PPN	1253:1255	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	41	from	chains	1202:1207	arg1	PPQ					1262:1264	PPQ	1262:1264	PPQ	1262:1264	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	41	from	chains	1202:1207	arg1	PPG					1248:1250	PPG	1248:1250	PPG	1248:1250	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	0	42	theme	Panax	56:60	arg1	species					62:68	Panax species	56:68	Panax species	56:68	Structural-fingerprinting of polysaccharides to discern Panax species by means of gas-liquid chromatography and mass spectrometry.					
32088239	1	43	from	ginseng	275:281	arg1	characterization					230:245	characterization	230:245	characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ)	230:334	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	5	44	theme	main	1234:1237	arg1	chain					1239:1243	the main chain	1230:1243	the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates	1230:1343	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	2	45	theme	parameters	380:389	arg1	reflection					348:357	the reflection	344:357	the reflection of stepped structure parameters	344:389	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	6	46	theme	high	1425:1428	arg1	comprehensibility					1430:1446	high comprehensibility	1425:1446	high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides	1425:1534	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	2	47	theme	structure	370:378	arg1	parameters					380:389	stepped structure parameters	362:389	stepped structure parameters	362:389	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	48	theme	compositional	526:538	arg1	MCF					556:558	MCF	556:558	MCF	556:558	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	48	theme	compositional	526:538	arg1	fingerprinting					540:553	monosaccharide compositional fingerprinting	511:553	monosaccharide compositional fingerprinting (MCF)	511:559	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	5	49	from	regions	1219:1225	arg1	PPN					1253:1255	PPN	1253:1255	PPN	1253:1255	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	49	from	regions	1219:1225	arg1	PPQ					1262:1264	PPQ	1262:1264	PPQ	1262:1264	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	49	from	regions	1219:1225	arg1	PPG					1248:1250	PPG	1248:1250	PPG	1248:1250	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	1	50	from	characterization	230:245	arg1	notoginseng					292:302	P. notoginseng	289:302	P. notoginseng (PN)	289:307	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	1	50	from	characterization	230:245	arg1	PG					284:285	PG	284:285	PG	284:285	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	1	50	from	characterization	230:245	arg1	quinquefolius					317:329	P. quinquefolius	314:329	P. quinquefolius (PQ)	314:334	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	1	50	from	characterization	230:245	arg1	ginseng					275:281	Panax ginseng	269:281	Panax ginseng (PG)	269:286	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	6	51	theme	experimental	1350:1361	arg1	results					1363:1369	The experimental results	1346:1369	The experimental results	1346:1369	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	5	52	theme	smooth	1212:1217	arg1	regions					1219:1225	smooth regions	1212:1225	smooth regions	1212:1225	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	6	53	theme	SFP	1402:1404	arg1	approach					1406:1413	the established SFP approach	1386:1413	the established SFP approach	1386:1413	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	5	54	theme	side	1197:1200	arg1	chains					1202:1207	side chains	1197:1207	side chains	1197:1207	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	55	from	PPQ	1262:1264	arg1	chains					1202:1207	side chains	1197:1207	side chains	1197:1207	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	55	from	PPQ	1262:1264	arg1	regions					1219:1225	smooth regions	1212:1225	smooth regions	1212:1225	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	2	56	theme	Smith	562:566	arg1	degradation					568:578	Smith degradation	562:578	Smith degradation	562:578	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	5	57	from	PPN	1253:1255	arg1	chains					1202:1207	side chains	1197:1207	side chains	1197:1207	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	57	from	PPN	1253:1255	arg1	regions					1219:1225	smooth regions	1212:1225	smooth regions	1212:1225	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	58	from	PPG	1248:1250	arg1	chains					1202:1207	side chains	1197:1207	side chains	1197:1207	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	58	from	PPG	1248:1250	arg1	regions					1219:1225	smooth regions	1212:1225	smooth regions	1212:1225	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	3	59	theme	Glc/Gal	941:947	arg1	ratio					932:936	the peak area ratio	918:936	the peak area ratio of Glc/Gal	918:947	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	3	59	theme	Glc/Gal	941:947	arg1	Rha					910:912	Rha	910:912	Rha	910:912	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	4	60	theme	component	1002:1010	arg1	analysis					1012:1019	principal component analysis	992:1019	principal component analysis	992:1019	Similarity analysis, heatmap analysis and principal component analysis were further performed to discern three Panax species based on SNF data sets.					
32088239	1	61	from	polysaccharides	250:264	arg1	notoginseng					292:302	P. notoginseng	289:302	P. notoginseng (PN)	289:307	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	1	61	from	polysaccharides	250:264	arg1	PG					284:285	PG	284:285	PG	284:285	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	1	61	from	polysaccharides	250:264	arg1	quinquefolius					317:329	P. quinquefolius	314:329	P. quinquefolius (PQ)	314:334	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	1	61	from	polysaccharides	250:264	arg1	ginseng					275:281	Panax ginseng	269:281	Panax ginseng (PG)	269:286	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	4	62	theme	data	1088:1091	arg1	sets					1093:1096	SNF data sets	1084:1096	SNF data sets	1084:1096	Similarity analysis, heatmap analysis and principal component analysis were further performed to discern three Panax species based on SNF data sets.					
32088239	1	63	theme	polysaccharides	250:264	arg1	characterization					230:245	characterization	230:245	characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ)	230:334	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	5	64	from	chain	1239:1243	arg1	PPN					1253:1255	PPN	1253:1255	PPN	1253:1255	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	64	from	chain	1239:1243	arg1	PPQ					1262:1264	PPQ	1262:1264	PPQ	1262:1264	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	64	from	chain	1239:1243	arg1	PPG					1248:1250	PPG	1248:1250	PPG	1248:1250	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	3	65	theme	peak	922:925	arg1	ratio					932:936	the peak area ratio	918:936	the peak area ratio of Glc/Gal	918:947	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	3	66	theme	PQ	819:820	arg1	similarity					798:807	the high interspecific similarity	775:807	the high interspecific similarity of PG and PQ	775:820	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	3	67	theme	area	927:930	arg1	ratio					932:936	the peak area ratio	918:936	the peak area ratio of Glc/Gal	918:947	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	6	68	theme	established	1390:1400	arg1	approach					1406:1413	the established SFP approach	1386:1413	the established SFP approach	1386:1413	The experimental results displayed that the established SFP approach possesses high comprehensibility as well as satisfactory generalization capability for analysis of plant polysaccharides.					
32088239	2	69	theme	resulting	396:404	arg1	profiles					418:425	the resulting integrative profiles	392:425	the resulting integrative profiles	392:425	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	2	69	theme	resulting	396:404	arg1	structural-fingerprinting					455:479	structural-fingerprinting	455:479	structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF)	455:683	Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF and SNF), and oligosaccharide compositional fingerprinting (OCF).					
32088239	3	70	theme	Rha	910:912	arg1	absence					899:905	absence	899:905	absence	899:905	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	3	70	theme	Rha	910:912	arg1	presence					887:894	the presence	883:894	the presence	883:894	The MCF, OCF and SDF did not allow for visual discrimination of the three species due to the high interspecific similarity of PG and PQ, whereas SNF could intuitively distinguish PG, PN, and PQ by the presence or absence of Rha and the peak area ratio of Glc/Gal.					
32088239	1	71	theme	sequential	148:157	arg1	chromatography					170:183	a sequential gas-liquid chromatography	146:183	a sequential gas-liquid chromatography	146:183	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	5	72	theme	common	1166:1171	arg1	backbones					1184:1192	the common structural backbones	1162:1192	the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates	1162:1343	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	72	theme	common	1166:1171	arg1	1 → structures					1120:1133	1 → structures	1120:1133	1 → structures	1120:1133	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	5	72	theme	common	1166:1171	arg1	-Hexp-					1113:1118	The linear →4)-Hexp-	1099:1118	The linear →4)-Hexp-	1099:1118	The linear →4)-Hexp-(1 → structures were clearly identified as the common structural backbones in side chains or smooth regions of the main chain in PPG, PPN, and PPQ using HILIC-UHPLC-ESI--MS/MS for characterization of partial acid hydrolyzates.					
32088239	1	73	theme	Panax	269:273	arg1	notoginseng					292:302	P. notoginseng	289:302	P. notoginseng (PN)	289:307	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	1	73	theme	Panax	269:273	arg1	PG					284:285	PG	284:285	PG	284:285	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	1	73	theme	Panax	269:273	arg1	quinquefolius					317:329	P. quinquefolius	314:329	P. quinquefolius (PQ)	314:334	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32088239	1	73	theme	Panax	269:273	arg1	ginseng					275:281	Panax ginseng	269:281	Panax ginseng (PG)	269:286	In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ).					
32603137	7	0	theme	sialic	1113:1118	arg1	residue					1125:1131	at least one sialic acid residue	1100:1131	at least one sialic acid residue	1100:1131	Importantly, all N-linked glycans detected contained at least one sialic acid residue, which are known to be labile.					
32603137	7	1	theme	N-linked	1064:1071	arg1	glycans					1073:1079	all N-linked glycans	1060:1079	all N-linked glycans detected	1060:1088	Importantly, all N-linked glycans detected contained at least one sialic acid residue, which are known to be labile.					
32603137	5	2	theme	Native	671:676	arg1	glycans					687:693	Native N-linked glycans	671:693	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin	671:756	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin were analyzed directly by IR-MALDESI in both positive and negative ionization mode.					
32603137	8	3	theme	first	1196:1200	arg1	step					1202:1205	a critical first step	1185:1205	a critical first step for N-linked glycan analysis	1185:1234	This work represents a critical first step for N-linked glycan analysis by IR-MALDESI with future efforts directed at mass spectrometry imaging.					
32603137	3	4	theme	N-linked	512:519	arg1	glycans					521:527	N-linked glycans	512:527	N-linked glycans	512:527	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	6	5	from	glycans	881:887	arg1	mode					901:904	negative mode	892:904	negative mode	892:904	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	5	6	theme	N-linked	678:685	arg1	glycans					687:693	Native N-linked glycans	671:693	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin	671:756	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin were analyzed directly by IR-MALDESI in both positive and negative ionization mode.					
32603137	2	7	theme	labile	307:312	arg1	character					314:322	labile character	307:322	labile character	307:322	Glycans are complex molecules that pose analytical challenges due to their isomeric compositions, labile character, and ionization preferences.					
32603137	6	8	theme	negative	892:899	arg1	mode					901:904	negative mode	892:904	negative mode	892:904	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	1	9	theme	Glycan	58:63	arg1	analysis					65:72	Glycan analysis	58:72	Glycan analysis by mass spectrometry	58:93	Glycan analysis by mass spectrometry has rapidly progressed due to the interest in understanding the role of glycans in disease and tumor progression.					
32603137	5	10	theme	bovine	744:749	arg1	fetuin					751:756	bovine fetuin	744:756	bovine fetuin	744:756	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin were analyzed directly by IR-MALDESI in both positive and negative ionization mode.					
32603137	3	11	theme	direct	493:498	arg1	analysis					500:507	the direct analysis	489:507	the direct analysis of N-linked glycans	489:527	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	5	12	link	N-linked	678:685	arg1	glycans					687:693	Native N-linked glycans	671:693	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin	671:756	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin were analyzed directly by IR-MALDESI in both positive and negative ionization mode.					
32603137	6	13	link	N-linked	912:919	arg1	glycans					921:927	4 N-linked glycans	910:927	4 N-linked glycans	910:927	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	8	14	theme	N-linked	1211:1218	arg1	analysis					1227:1234	N-linked glycan analysis	1211:1234	N-linked glycan analysis	1211:1234	This work represents a critical first step for N-linked glycan analysis by IR-MALDESI with future efforts directed at mass spectrometry imaging.					
32603137	5	15	theme	fetuin	751:756	arg1	cleavage					717:724	enzymatic cleavage	707:724	enzymatic cleavage (via PNGase F) of bovine fetuin	707:756	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin were analyzed directly by IR-MALDESI in both positive and negative ionization mode.					
32603137	8	16	with	IR-MALDESI	1239:1248	arg1	efforts					1262:1268	future efforts	1255:1268	future efforts directed at mass spectrometry imaging	1255:1306	This work represents a critical first step for N-linked glycan analysis by IR-MALDESI with future efforts directed at mass spectrometry imaging.					
32603137	0	17	theme	Glycans	35:41	arg1	Analysis					7:14	Direct Analysis	0:14	Direct Analysis of Native N-Linked Glycans by IR-MALDESI.	0:56	Direct Analysis of Native N-Linked Glycans by IR-MALDESI.					
32603137	0	18	theme	Direct	0:5	arg1	Analysis					7:14	Direct Analysis	0:14	Direct Analysis of Native N-Linked Glycans by IR-MALDESI.	0:56	Direct Analysis of Native N-Linked Glycans by IR-MALDESI.					
32603137	6	19	theme	N-linked	872:879	arg1	glycans					881:887	12 N-linked glycans	869:887	12 N-linked glycans in negative mode	869:904	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	4	20	gly	sialylated	651:660	arg1	glycans					662:668	sialylated glycans	651:668	sialylated glycans	651:668	The glycoprotein bovine fetuin was chosen for this analysis as its glycome is well-characterized and heavily composed of sialylated glycans.					
32603137	1	21	theme	mass	77:80	arg1	spectrometry					82:93	mass spectrometry	77:93	mass spectrometry	77:93	Glycan analysis by mass spectrometry has rapidly progressed due to the interest in understanding the role of glycans in disease and tumor progression.					
32603137	1	22	theme	glycans	167:173	arg1	role					159:162	the role	155:162	the role of glycans in disease and tumor progression	155:206	Glycan analysis by mass spectrometry has rapidly progressed due to the interest in understanding the role of glycans in disease and tumor progression.					
32603137	3	23	theme	infrared	386:393	arg1	IR-MALDESI					453:462	IR-MALDESI	453:462	IR-MALDESI	453:462	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	23	theme	infrared	386:393	arg1	ionization					441:450	infrared matrix-assisted laser desorption electrospray ionization	386:450	infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)	386:463	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	23	theme	infrared	386:393	arg1	approach					476:483	a novel approach	468:483	a novel approach for the direct analysis of N-linked glycans	468:527	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	7	24	theme	acid	1120:1123	arg1	residue					1125:1131	at least one sialic acid residue	1100:1131	at least one sialic acid residue	1100:1131	Importantly, all N-linked glycans detected contained at least one sialic acid residue, which are known to be labile.					
32603137	0	25	theme	Native	19:24	arg1	Glycans					35:41	Native N-Linked Glycans	19:41	Native N-Linked Glycans	19:41	Direct Analysis of Native N-Linked Glycans by IR-MALDESI.					
32603137	5	26	theme	negative	816:823	arg1	mode					836:839	both positive and negative ionization mode	798:839	both positive and negative ionization mode	798:839	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin were analyzed directly by IR-MALDESI in both positive and negative ionization mode.					
32603137	8	27	theme	critical	1187:1194	arg1	step					1202:1205	a critical first step	1185:1205	a critical first step for N-linked glycan analysis	1185:1234	This work represents a critical first step for N-linked glycan analysis by IR-MALDESI with future efforts directed at mass spectrometry imaging.					
32603137	3	28	theme	novel	470:474	arg1	ionization					441:450	infrared matrix-assisted laser desorption electrospray ionization	386:450	infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)	386:463	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	28	theme	novel	470:474	arg1	approach					476:483	a novel approach	468:483	a novel approach for the direct analysis of N-linked glycans	468:527	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	29	link	N-linked	512:519	arg1	glycans					521:527	N-linked glycans	512:527	N-linked glycans	512:527	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	6	30	from	increase	961:968	arg1	amount					977:982	the amount	973:982	the amount of underivatized glycans detected by other ionization sources	973:1044	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	6	30	from	increase	961:968	arg1	glycans					1001:1007	underivatized glycans	987:1007	underivatized glycans	987:1007	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	2	31	theme	isomeric	284:291	arg1	compositions					293:304	their isomeric compositions	278:304	their isomeric compositions	278:304	Glycans are complex molecules that pose analytical challenges due to their isomeric compositions, labile character, and ionization preferences.					
32603137	6	32	link	N-linked	872:879	arg1	glycans					881:887	12 N-linked glycans	869:887	12 N-linked glycans in negative mode	869:904	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	3	33	theme	desorption	417:426	arg1	IR-MALDESI					453:462	IR-MALDESI	453:462	IR-MALDESI	453:462	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	33	theme	desorption	417:426	arg1	ionization					441:450	infrared matrix-assisted laser desorption electrospray ionization	386:450	infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)	386:463	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	33	theme	desorption	417:426	arg1	approach					476:483	a novel approach	468:483	a novel approach for the direct analysis of N-linked glycans	468:527	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	5	34	theme	PNGase	731:736	arg1	F					738:738	PNGase F	731:738	PNGase F	731:738	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin were analyzed directly by IR-MALDESI in both positive and negative ionization mode.					
32603137	6	35	theme	ionization	1027:1036	arg1	sources					1038:1044	other ionization sources	1021:1044	other ionization sources	1021:1044	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	2	36	theme	complex	221:227	arg1	Glycans					209:215	Glycans	209:215	Glycans	209:215	Glycans are complex molecules that pose analytical challenges due to their isomeric compositions, labile character, and ionization preferences.					
32603137	2	36	theme	complex	221:227	arg1	molecules					229:237	complex molecules	221:237	complex molecules that pose analytical challenges due to their isomeric compositions, labile character, and ionization preferences	221:350	Glycans are complex molecules that pose analytical challenges due to their isomeric compositions, labile character, and ionization preferences.					
32603137	8	37	theme	glycan	1220:1225	arg1	analysis					1227:1234	N-linked glycan analysis	1211:1234	N-linked glycan analysis	1211:1234	This work represents a critical first step for N-linked glycan analysis by IR-MALDESI with future efforts directed at mass spectrometry imaging.					
32603137	1	38	theme	tumor	190:194	arg1	progression					196:206	tumor progression	190:206	tumor progression	190:206	Glycan analysis by mass spectrometry has rapidly progressed due to the interest in understanding the role of glycans in disease and tumor progression.					
32603137	0	39	theme	N-Linked	26:33	arg1	Glycans					35:41	Native N-Linked Glycans	19:41	Native N-Linked Glycans	19:41	Direct Analysis of Native N-Linked Glycans by IR-MALDESI.					
32603137	4	40	theme	glycoprotein	534:545	arg1	fetuin					554:559	The glycoprotein bovine fetuin	530:559	The glycoprotein bovine fetuin	530:559	The glycoprotein bovine fetuin was chosen for this analysis as its glycome is well-characterized and heavily composed of sialylated glycans.					
32603137	3	41	theme	electrospray	428:439	arg1	IR-MALDESI					453:462	IR-MALDESI	453:462	IR-MALDESI	453:462	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	41	theme	electrospray	428:439	arg1	ionization					441:450	infrared matrix-assisted laser desorption electrospray ionization	386:450	infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)	386:463	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	41	theme	electrospray	428:439	arg1	approach					476:483	a novel approach	468:483	a novel approach for the direct analysis of N-linked glycans	468:527	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	7	42	link	N-linked	1064:1071	arg1	glycans					1073:1079	all N-linked glycans	1060:1079	all N-linked glycans detected	1060:1088	Importantly, all N-linked glycans detected contained at least one sialic acid residue, which are known to be labile.					
32603137	5	43	theme	ionization	825:834	arg1	mode					836:839	both positive and negative ionization mode	798:839	both positive and negative ionization mode	798:839	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin were analyzed directly by IR-MALDESI in both positive and negative ionization mode.					
32603137	4	44	theme	sialylated	651:660	arg1	glycans					662:668	sialylated glycans	651:668	sialylated glycans	651:668	The glycoprotein bovine fetuin was chosen for this analysis as its glycome is well-characterized and heavily composed of sialylated glycans.					
32603137	3	45	theme	matrix-assisted	395:409	arg1	IR-MALDESI					453:462	IR-MALDESI	453:462	IR-MALDESI	453:462	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	45	theme	matrix-assisted	395:409	arg1	ionization					441:450	infrared matrix-assisted laser desorption electrospray ionization	386:450	infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)	386:463	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	45	theme	matrix-assisted	395:409	arg1	approach					476:483	a novel approach	468:483	a novel approach for the direct analysis of N-linked glycans	468:527	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	6	46	theme	positive	932:939	arg1	mode					941:944	positive mode	932:944	positive mode	932:944	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	5	47	theme	positive	803:810	arg1	mode					836:839	both positive and negative ionization mode	798:839	both positive and negative ionization mode	798:839	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin were analyzed directly by IR-MALDESI in both positive and negative ionization mode.					
32603137	6	48	theme	significant	949:959	arg1	increase					961:968	a significant increase	947:968	a significant increase in the amount of underivatized glycans detected by other ionization sources	947:1044	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	2	49	theme	ionization	329:338	arg1	preferences					340:350	ionization preferences	329:350	ionization preferences	329:350	Glycans are complex molecules that pose analytical challenges due to their isomeric compositions, labile character, and ionization preferences.					
32603137	3	50	theme	laser	411:415	arg1	IR-MALDESI					453:462	IR-MALDESI	453:462	IR-MALDESI	453:462	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	50	theme	laser	411:415	arg1	ionization					441:450	infrared matrix-assisted laser desorption electrospray ionization	386:450	infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)	386:463	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	3	50	theme	laser	411:415	arg1	approach					476:483	a novel approach	468:483	a novel approach for the direct analysis of N-linked glycans	468:527	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	6	51	from	glycans	921:927	arg1	mode					901:904	negative mode	892:904	negative mode	892:904	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	8	52	link	N-linked	1211:1218	arg1	analysis					1227:1234	N-linked glycan analysis	1211:1234	N-linked glycan analysis	1211:1234	This work represents a critical first step for N-linked glycan analysis by IR-MALDESI with future efforts directed at mass spectrometry imaging.					
32603137	4	53	theme	bovine	547:552	arg1	fetuin					554:559	The glycoprotein bovine fetuin	530:559	The glycoprotein bovine fetuin	530:559	The glycoprotein bovine fetuin was chosen for this analysis as its glycome is well-characterized and heavily composed of sialylated glycans.					
32603137	5	54	theme	enzymatic	707:715	arg1	cleavage					717:724	enzymatic cleavage	707:724	enzymatic cleavage (via PNGase F) of bovine fetuin	707:756	Native N-linked glycans produced by enzymatic cleavage (via PNGase F) of bovine fetuin were analyzed directly by IR-MALDESI in both positive and negative ionization mode.					
32603137	2	55	theme	analytical	249:258	arg1	challenges					260:269	analytical challenges	249:269	analytical challenges due to their isomeric compositions, labile character, and ionization preferences	249:350	Glycans are complex molecules that pose analytical challenges due to their isomeric compositions, labile character, and ionization preferences.					
32603137	8	56	theme	mass	1282:1285	arg1	spectrometry					1287:1298	mass spectrometry	1282:1298	mass spectrometry imaging	1282:1306	This work represents a critical first step for N-linked glycan analysis by IR-MALDESI with future efforts directed at mass spectrometry imaging.					
32603137	6	57	theme	other	1021:1025	arg1	sources					1038:1044	other ionization sources	1021:1044	other ionization sources	1021:1044	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	4	58	gly	glycoprotein	534:545	arg1	glycoprotein					534:545	The glycoprotein bovine fetuin	530:559	The glycoprotein bovine fetuin	530:559	The glycoprotein bovine fetuin was chosen for this analysis as its glycome is well-characterized and heavily composed of sialylated glycans.					
32603137	3	59	theme	glycans	521:527	arg1	analysis					500:507	the direct analysis	489:507	the direct analysis of N-linked glycans	489:527	This study sought to demonstrate infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) as a novel approach for the direct analysis of N-linked glycans.					
32603137	7	60	contain	contained	1090:1098	arg2	residue					1125:1131	at least one sialic acid residue	1100:1131	at least one sialic acid residue	1100:1131	Importantly, all N-linked glycans detected contained at least one sialic acid residue, which are known to be labile.					
32603137	7	60	contain	contained	1090:1098	arg1	glycans					1073:1079	all N-linked glycans	1060:1079	all N-linked glycans detected	1060:1088	Importantly, all N-linked glycans detected contained at least one sialic acid residue, which are known to be labile.					
32603137	6	61	theme	glycans	1001:1007	arg1	amount					977:982	the amount	973:982	the amount of underivatized glycans detected by other ionization sources	973:1044	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	6	61	theme	glycans	1001:1007	arg1	glycans					1001:1007	underivatized glycans	987:1007	underivatized glycans	987:1007	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	6	62	theme	N-linked	912:919	arg1	glycans					921:927	4 N-linked glycans	910:927	4 N-linked glycans	910:927	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
32603137	8	63	theme	future	1255:1260	arg1	efforts					1262:1268	future efforts	1255:1268	future efforts directed at mass spectrometry imaging	1255:1306	This work represents a critical first step for N-linked glycan analysis by IR-MALDESI with future efforts directed at mass spectrometry imaging.					
32603137	1	64	from	role	159:162	arg1	disease					178:184	disease	178:184	disease	178:184	Glycan analysis by mass spectrometry has rapidly progressed due to the interest in understanding the role of glycans in disease and tumor progression.					
32603137	1	64	from	role	159:162	arg1	progression					196:206	tumor progression	190:206	tumor progression	190:206	Glycan analysis by mass spectrometry has rapidly progressed due to the interest in understanding the role of glycans in disease and tumor progression.					
32603137	6	65	theme	underivatized	987:999	arg1	glycans					1001:1007	underivatized glycans	987:1007	underivatized glycans	987:1007	In this study, we detected 12 N-linked glycans in negative mode and 4 N-linked glycans in positive mode, a significant increase in the amount of underivatized glycans detected by other ionization sources.					
33164360	0	0	theme	polysaccharide	88:101	arg1	structures					31:40	chemical structures	22:40	chemical structures	22:40	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	0	0	theme	polysaccharide	88:101	arg1	activities					62:71	pharmacological activities	46:71	pharmacological activities	46:71	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	1	1	theme	medicinal	147:155	arg1	herb					176:179	a both medicinal and edible Chinese herb	140:179	a both medicinal and edible Chinese herb variety	140:187	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	1	1	theme	medicinal	147:155	arg1	cocos					131:135	Poria cocos	125:135	Poria cocos	125:135	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	4	2	from	progress	682:689	arg1	years					758:762	recent years	751:762	recent years	751:762	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	4	2	from	progress	682:689	arg1	activities					710:719	pharmacological activities	694:719	pharmacological activities of PCP and its derivatives	694:746	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	0	3	theme	cocos	82:86	arg1	polysaccharide					88:101	Poria cocos polysaccharide	76:101	Poria cocos polysaccharide	76:101	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	0	4	from	progress	10:17	arg1	structures					31:40	chemical structures	22:40	chemical structures	22:40	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	0	4	from	progress	10:17	arg1	activities					62:71	pharmacological activities	46:71	pharmacological activities	46:71	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	2	5	theme	substances	321:330	arg1	one					288:290	one	288:290	one	288:290	P. cocos polysaccharide(PCP) is one of the most important active substances of P. cocos.					
33164360	2	5	theme	substances	321:330	arg1	substances					321:330	the most important active substances	295:330	the most important active substances of P. cocos	295:342	P. cocos polysaccharide(PCP) is one of the most important active substances of P. cocos.					
33164360	1	6	theme	tranquilizing	241:253	arg1	effects					199:205	the effects	195:205	the effects of diuretic, spleen-enhancing and tranquilizing	195:253	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	5	7	theme	structure-activity	952:969	arg1	relationship					971:982	the structure-activity relationship	948:982	the structure-activity relationship of PCP	948:989	In addition, the current problems were discussed in this article to provide reference for further exploration of the structure-activity relationship of PCP and its further development and utilization.					
33164360	4	8	theme	derivatives	736:746	arg1	activities					710:719	pharmacological activities	694:719	pharmacological activities of PCP and its derivatives	694:746	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	4	9	theme	recent	751:756	arg1	years					758:762	recent years	751:762	recent years	751:762	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	3	10	dep	development	538:548	arg1	value					566:570	value	566:570	value	566:570	Pharmacological research shows that PCP has a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection, with a huge development and utilization value.					
33164360	3	11	contain	has	385:387	arg1	PCP					381:383	PCP	381:383	PCP	381:383	Pharmacological research shows that PCP has a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection, with a huge development and utilization value.					
33164360	3	11	contain	has	385:387	arg2	activities					418:427	pharmacological activities	402:427	pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection	402:523	Pharmacological research shows that PCP has a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection, with a huge development and utilization value.					
33164360	3	11	contain	has	385:387	arg2	variety					391:397	a variety	389:397	a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection	389:523	Pharmacological research shows that PCP has a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection, with a huge development and utilization value.					
33164360	3	12	theme	liver	508:512	arg1	protection					514:523	liver protection	508:523	liver protection	508:523	Pharmacological research shows that PCP has a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection, with a huge development and utilization value.					
33164360	4	13	from	structures	654:663	arg1	years					758:762	recent years	751:762	recent years	751:762	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	4	13	from	structures	654:663	arg1	activities					710:719	pharmacological activities	694:719	pharmacological activities of PCP and its derivatives	694:746	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	1	14	theme	edible	161:166	arg1	herb					176:179	a both medicinal and edible Chinese herb	140:179	a both medicinal and edible Chinese herb variety	140:187	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	1	14	theme	edible	161:166	arg1	cocos					131:135	Poria cocos	125:135	Poria cocos	125:135	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	0	15	theme	derivatives	111:121	arg1	structures					31:40	chemical structures	22:40	chemical structures	22:40	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	0	15	theme	derivatives	111:121	arg1	activities					62:71	pharmacological activities	46:71	pharmacological activities	46:71	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	2	16	theme	cocos	259:263	arg1	PCP					280:282	PCP	280:282	PCP	280:282	P. cocos polysaccharide(PCP) is one of the most important active substances of P. cocos.					
33164360	2	16	theme	cocos	259:263	arg1	polysaccharide					265:278	P. cocos polysaccharide	256:278	P. cocos polysaccharide(PCP)	256:283	P. cocos polysaccharide(PCP) is one of the most important active substances of P. cocos.					
33164360	1	17	theme	Chinese	168:174	arg1	herb					176:179	a both medicinal and edible Chinese herb	140:179	a both medicinal and edible Chinese herb variety	140:187	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	1	17	theme	Chinese	168:174	arg1	cocos					131:135	Poria cocos	125:135	Poria cocos	125:135	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	5	18	theme	further	925:931	arg1	exploration					933:943	further exploration	925:943	further exploration of the structure-activity relationship of PCP and its further development and utilization	925:1033	In addition, the current problems were discussed in this article to provide reference for further exploration of the structure-activity relationship of PCP and its further development and utilization.					
33164360	0	19	theme	[Research	0:8	arg1	progress					10:17	[Research progress	0:17	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives	0:121	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	4	20	theme	chemical	645:652	arg1	structures					654:663	the chemical structures	641:663	the chemical structures	641:663	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	5	21	theme	current	852:858	arg1	problems					860:867	the current problems	848:867	the current problems	848:867	In addition, the current problems were discussed in this article to provide reference for further exploration of the structure-activity relationship of PCP and its further development and utilization.					
33164360	0	22	theme	chemical	22:29	arg1	structures					31:40	chemical structures	22:40	chemical structures	22:40	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	3	23	theme	Pharmacological	345:359	arg1	research					361:368	Pharmacological research	345:368	Pharmacological research	345:368	Pharmacological research shows that PCP has a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection, with a huge development and utilization value.					
33164360	3	24	theme	huge	533:536	arg1	development					538:548	a huge development	531:548	a huge development	531:548	Pharmacological research shows that PCP has a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection, with a huge development and utilization value.					
33164360	4	25	theme	research	673:680	arg1	progress					682:689	the research progress	669:689	the research progress	669:689	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	2	26	theme	important	304:312	arg1	substances					321:330	the most important active substances	295:330	the most important active substances of P. cocos	295:342	P. cocos polysaccharide(PCP) is one of the most important active substances of P. cocos.					
33164360	1	27	dep	herb	176:179	arg1	variety					181:187	variety	181:187	a both medicinal and edible Chinese herb variety	140:187	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	3	28	theme	pharmacological	402:416	arg1	activities					418:427	pharmacological activities	402:427	pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection	402:523	Pharmacological research shows that PCP has a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection, with a huge development and utilization value.					
33164360	4	29	theme	pharmacological	694:708	arg1	activities					710:719	pharmacological activities	694:719	pharmacological activities of PCP and its derivatives	694:746	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	1	30	with	herb	176:179	arg1	effects					199:205	the effects	195:205	the effects of diuretic, spleen-enhancing and tranquilizing	195:253	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	5	31	theme	relationship	971:982	arg1	exploration					933:943	further exploration	925:943	further exploration of the structure-activity relationship of PCP and its further development and utilization	925:1033	In addition, the current problems were discussed in this article to provide reference for further exploration of the structure-activity relationship of PCP and its further development and utilization.					
33164360	2	32	theme	active	314:319	arg1	substances					321:330	the most important active substances	295:330	the most important active substances of P. cocos	295:342	P. cocos polysaccharide(PCP) is one of the most important active substances of P. cocos.					
33164360	0	33	theme	pharmacological	46:60	arg1	activities					62:71	pharmacological activities	46:71	pharmacological activities	46:71	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	4	34	theme	PCP	819:821	arg1	compositions					788:799	the chemical compositions	775:799	the chemical compositions	775:799	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	4	34	theme	PCP	819:821	arg1	activities					805:814	activities	805:814	activities	805:814	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	1	35	theme	Poria	125:129	arg1	cocos					131:135	Poria cocos	125:135	Poria cocos	125:135	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	1	35	theme	Poria	125:129	arg1	herb					176:179	a both medicinal and edible Chinese herb	140:179	a both medicinal and edible Chinese herb variety	140:187	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	5	36	theme	development	1007:1017	arg1	exploration					933:943	further exploration	925:943	further exploration of the structure-activity relationship of PCP and its further development and utilization	925:1033	In addition, the current problems were discussed in this article to provide reference for further exploration of the structure-activity relationship of PCP and its further development and utilization.					
33164360	4	37	theme	chemical	779:786	arg1	compositions					788:799	the chemical compositions	775:799	the chemical compositions	775:799	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	2	38	theme	P.	256:257	arg1	PCP					280:282	PCP	280:282	PCP	280:282	P. cocos polysaccharide(PCP) is one of the most important active substances of P. cocos.					
33164360	2	38	theme	P.	256:257	arg1	polysaccharide					265:278	P. cocos polysaccharide	256:278	P. cocos polysaccharide(PCP)	256:283	P. cocos polysaccharide(PCP) is one of the most important active substances of P. cocos.					
33164360	4	39	theme	PCP	724:726	arg1	activities					710:719	pharmacological activities	694:719	pharmacological activities of PCP and its derivatives	694:746	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	5	40	theme	PCP	987:989	arg1	relationship					971:982	the structure-activity relationship	948:982	the structure-activity relationship of PCP	948:989	In addition, the current problems were discussed in this article to provide reference for further exploration of the structure-activity relationship of PCP and its further development and utilization.					
33164360	5	40	theme	PCP	987:989	arg1	development					1007:1017	its further development	995:1017	its further development	995:1017	In addition, the current problems were discussed in this article to provide reference for further exploration of the structure-activity relationship of PCP and its further development and utilization.					
33164360	5	40	theme	PCP	987:989	arg1	utilization					1023:1033	utilization	1023:1033	utilization	1023:1033	In addition, the current problems were discussed in this article to provide reference for further exploration of the structure-activity relationship of PCP and its further development and utilization.					
33164360	1	41	theme	diuretic	210:217	arg1	effects					199:205	the effects	195:205	the effects of diuretic, spleen-enhancing and tranquilizing	195:253	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
33164360	0	42	theme	Poria	76:80	arg1	polysaccharide					88:101	Poria cocos polysaccharide	76:101	Poria cocos polysaccharide	76:101	[Research progress on chemical structures and pharmacological activities of Poria cocos polysaccharide and its derivatives].					
33164360	5	43	theme	further	999:1005	arg1	development					1007:1017	its further development	995:1017	its further development	995:1017	In addition, the current problems were discussed in this article to provide reference for further exploration of the structure-activity relationship of PCP and its further development and utilization.					
33164360	2	44	theme	cocos	338:342	arg1	substances					321:330	the most important active substances	295:330	the most important active substances of P. cocos	295:342	P. cocos polysaccharide(PCP) is one of the most important active substances of P. cocos.					
33164360	3	45	theme	activities	418:427	arg1	variety					391:397	a variety	389:397	a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection	389:523	Pharmacological research shows that PCP has a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection, with a huge development and utilization value.					
33164360	3	45	theme	activities	418:427	arg1	activities					418:427	pharmacological activities	402:427	pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection	402:523	Pharmacological research shows that PCP has a variety of pharmacological activities such as regulating immunity, anti-inflammatory, anti-oxidation, antitumor, and liver protection, with a huge development and utilization value.					
33164360	4	46	theme	relevant	591:598	arg1	literature					600:609	the relevant literature	587:609	the relevant literature	587:609	By consulting the relevant literature, we systematically summarized the chemical structures and the research progress on pharmacological activities of PCP and its derivatives in recent years to explore the chemical compositions and activities of PCP thoroughly.					
33164360	2	47	theme	P.	335:336	arg1	cocos					338:342	P. cocos	335:342	P. cocos	335:342	P. cocos polysaccharide(PCP) is one of the most important active substances of P. cocos.					
33164360	1	48	theme	spleen-enhancing	220:235	arg1	effects					199:205	the effects	195:205	the effects of diuretic, spleen-enhancing and tranquilizing	195:253	Poria cocos is a both medicinal and edible Chinese herb variety, with the effects of diuretic, spleen-enhancing and tranquilizing.					
34051261	1	0	theme	polyelectrolyte	199:213	arg1	PEC					224:226	PEC	224:226	PEC	224:226	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	1	0	theme	polyelectrolyte	199:213	arg1	complex					215:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex	149:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	149:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	2	1	theme	composite	554:562	arg1	fiber					564:568	the composite fiber	550:568	the composite fiber	550:568	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	3	2	theme	SA	693:694	arg1	ratio					684:688	the mass ratio	675:688	the mass ratio of SA to QAC	675:701	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	7	3	theme	antibacterial	1536:1548	arg1	properties					1550:1559	its mechanical, natural, and antibacterial properties	1507:1559	its mechanical, natural, and antibacterial properties	1507:1559	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	3	4	with	solution	825:832	arg1	precipitate					850:860	flocculent precipitate	839:860	flocculent precipitate	839:860	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	7	5	theme	absorbable	1646:1655	arg1	suture					1657:1662	medical absorbable suture	1638:1662	medical absorbable suture	1638:1662	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	5	6	with	solution	1038:1045	arg1	ratio					1061:1065	the mass ratio	1052:1065	the mass ratio of SA to QAC 10/1 and concentration 5.5 wt%	1052:1109	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	0	7	theme	alginate/chitosan	67:83	arg1	fibers					106:111	alginate/chitosan derivative composite fibers	67:111	alginate/chitosan derivative composite fibers	67:111	Study on polysaccharide polyelectrolyte complex and fabrication of alginate/chitosan derivative composite fibers.					
34051261	6	8	theme	EDS	1349:1351	arg1	determination					1353:1365	The FT-IR and EDS determination	1335:1365	determination	1353:1365	The FT-IR and EDS determination indicated there formed egg-box between SA and Ca2+, cross-linked network between glutaraldehyde(GA) and SA, QAC, respectively.					
34051261	4	9	from	salt	994:997	arg1	solution					1002:1009	solution	1002:1009	solution	1002:1009	Moreover, the electrical potential also depended on salt in solution.					
34051261	7	10	theme	medical	1623:1629	arg1	gauze					1631:1635	medical gauze	1623:1635	medical gauze	1623:1635	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	2	11	theme	DV-C	495:498	arg1	Rheometer					507:515	DV-C Rotary Rheometer	495:515	DV-C Rotary Rheometer	495:515	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	3	12	theme	solution	755:762	arg1	state					735:739	the state	731:739	the state of the binary solution in water	731:771	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	0	13	theme	composite	96:104	arg1	fibers					106:111	alginate/chitosan derivative composite fibers	67:111	alginate/chitosan derivative composite fibers	67:111	Study on polysaccharide polyelectrolyte complex and fabrication of alginate/chitosan derivative composite fibers.					
34051261	1	14	theme	SA/QAC	347:352	arg1	fibers					364:369	SA/QAC composite fibers	347:369	SA/QAC composite fibers	347:369	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	3	15	theme	uniform	798:804	arg1	solution					806:813	transparent uniform solution	786:813	transparent uniform solution	786:813	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	2	16	theme	single	619:624	arg1	meter					641:645	YG004 single fiber strength meter	613:645	YG004 single fiber strength meter	613:645	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	5	17	theme	breaking	1176:1183	arg1	cN·dtex-1					1199:1207	breaking strength 2.37 cN·dtex-1	1176:1207	breaking strength 2.37 cN·dtex-1	1176:1207	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	3	18	theme	flocculent	839:848	arg1	precipitate					850:860	flocculent precipitate	839:860	flocculent precipitate	839:860	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	5	19	theme	good	1241:1244	arg1	properties					1276:1285	good antibacterial and hydrophobic properties	1241:1285	good antibacterial and hydrophobic properties	1241:1285	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	5	20	theme	PEC	1034:1036	arg1	solution					1038:1045	this uniform PEC solution	1021:1045	this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%	1021:1238	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	4	21	theme	electrical	956:965	arg1	potential					967:975	the electrical potential	952:975	the electrical potential	952:975	Moreover, the electrical potential also depended on salt in solution.					
34051261	1	22	theme	cationic	294:301	arg1	polyelectrolytes					303:318	anionic and cationic polyelectrolytes	282:318	anionic and cationic polyelectrolytes	282:318	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	5	23	from	solution	1038:1045	arg1	water					1114:1118	water	1114:1118	water	1114:1118	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	5	23	from	solution	1038:1045	arg1	fibers					1138:1143	SA/QAC composite fibers	1121:1143	SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1	1121:1207	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	5	23	from	solution	1038:1045	arg1	elongation					1222:1231	breaking elongation	1213:1231	breaking elongation	1213:1231	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	1	24	theme	Sodium	114:119	arg1	blending					135:142	Sodium alginate (SA) blending	114:142	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	114:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	7	25	theme	composite	1573:1581	arg1	fiber					1583:1587	the SA/QAC composite fiber	1562:1587	the SA/QAC composite fiber	1562:1587	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	2	26	theme	strength	632:639	arg1	meter					641:645	YG004 single fiber strength meter	613:645	YG004 single fiber strength meter	613:645	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	2	27	theme	solution	421:428	arg1	charge					386:391	The potential charge	372:391	The potential charge	372:391	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	2	27	theme	solution	421:428	arg1	rheology					401:408	the rheology	397:408	the rheology of the PEC solution	397:428	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	0	28	theme	polysaccharide	9:22	arg1	complex					40:46	polysaccharide polyelectrolyte complex	9:46	polysaccharide polyelectrolyte complex	9:46	Study on polysaccharide polyelectrolyte complex and fabrication of alginate/chitosan derivative composite fibers.					
34051261	5	29	theme	concentration	1089:1101	arg1	%					1109:1109	concentration 5.5 wt%	1089:1109	concentration 5.5 wt%	1089:1109	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	6	30	theme	FT-IR	1339:1343	arg1	determination					1353:1365	The FT-IR and EDS determination	1335:1365	determination	1353:1365	The FT-IR and EDS determination indicated there formed egg-box between SA and Ca2+, cross-linked network between glutaraldehyde(GA) and SA, QAC, respectively.					
34051261	1	31	theme	polyelectrolytes	303:318	arg1	blending					270:277	the binary blending	259:277	the binary blending of anionic and cationic polyelectrolytes	259:318	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	2	32	dep	structure	522:530	arg1	the					518:520	the	518:520	the	518:520	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	2	33	theme	Particle	468:475	arg1	Analyzer					482:489	Zeta Laser Particle Size Analyzer	457:489	Zeta Laser Particle Size Analyzer	457:489	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	5	34	theme	green	1307:1311	arg1	process					1326:1332	green wet-spinning process	1307:1332	green wet-spinning process	1307:1332	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	3	35	theme	uniform	876:882	arg1	solution					884:891	uniform solution	876:891	uniform solution	876:891	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	3	36	from	state	735:739	arg1	water					767:771	water	767:771	water	767:771	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	5	37	theme	SA/QAC	1121:1126	arg1	fibers					1138:1143	SA/QAC composite fibers	1121:1143	SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1	1121:1207	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	3	38	theme	electrical	913:922	arg1	change					934:939	the electrical potential change	909:939	the electrical potential change	909:939	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	1	39	theme	ammonium	160:167	arg1	PEC					224:226	PEC	224:226	PEC	224:226	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	1	39	theme	ammonium	160:167	arg1	complex					215:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex	149:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	149:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	2	40	theme	potential	376:384	arg1	charge					386:391	The potential charge	372:391	The potential charge	372:391	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	1	41	theme	binary	263:268	arg1	blending					270:277	the binary blending	259:277	the binary blending of anionic and cationic polyelectrolytes	259:318	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	1	42	theme	QAC	179:181	arg1	PEC					224:226	PEC	224:226	PEC	224:226	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	1	42	theme	QAC	179:181	arg1	complex					215:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex	149:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	149:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	7	43	theme	tissue	1669:1674	arg1	engineering					1676:1686	tissue engineering	1669:1686	tissue engineering	1669:1686	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	3	44	theme	mass	679:682	arg1	ratio					684:688	the mass ratio	675:688	the mass ratio of SA to QAC	675:701	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	1	45	theme	polysaccharide	184:197	arg1	PEC					224:226	PEC	224:226	PEC	224:226	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	1	45	theme	polysaccharide	184:197	arg1	complex					215:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex	149:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	149:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	5	46	theme	mass	1056:1059	arg1	ratio					1061:1065	the mass ratio	1052:1065	the mass ratio of SA to QAC 10/1 and concentration 5.5 wt%	1052:1109	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	1	47	theme	complex	215:221	arg1	system					229:234	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	149:234	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	149:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	1	48	from	research	250:257	arg1	detail					323:328	detail	323:328	detail	323:328	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	7	49	theme	natural	1523:1529	arg1	properties					1550:1559	its mechanical, natural, and antibacterial properties	1507:1559	its mechanical, natural, and antibacterial properties	1507:1559	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	5	50	with	elongation	1222:1231	arg1	performances					1160:1171	excellent performances	1150:1171	excellent performances of breaking strength 2.37 cN·dtex-1	1150:1207	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	1	51	theme	SA	131:132	arg1	blending					135:142	Sodium alginate (SA) blending	114:142	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	114:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	7	52	theme	mechanical	1511:1520	arg1	properties					1550:1559	its mechanical, natural, and antibacterial properties	1507:1559	its mechanical, natural, and antibacterial properties	1507:1559	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	7	53	contain	has	1589:1591	arg2	advantages					1593:1602	advantages	1593:1602	advantages	1593:1602	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	7	53	contain	has	1589:1591	arg1	fiber					1583:1587	the SA/QAC composite fiber	1562:1587	the SA/QAC composite fiber	1562:1587	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	5	54	dep	water	1114:1118	arg1	%					1238:1238	14.11%	1233:1238	14.11%	1233:1238	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	5	55	theme	strength	1185:1192	arg1	cN·dtex-1					1199:1207	breaking strength 2.37 cN·dtex-1	1176:1207	breaking strength 2.37 cN·dtex-1	1176:1207	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	3	56	theme	transparent	786:796	arg1	solution					806:813	transparent uniform solution	786:813	transparent uniform solution	786:813	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	2	57	theme	fiber	564:568	arg1	properties					536:545	properties	536:545	properties	536:545	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	2	57	theme	fiber	564:568	arg1	structure					522:530	structure	522:530	structure	522:530	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	5	58	theme	cN·dtex-1	1199:1207	arg1	performances					1160:1171	excellent performances	1150:1171	excellent performances of breaking strength 2.37 cN·dtex-1	1150:1207	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	0	59	theme	derivative	85:94	arg1	fibers					106:111	alginate/chitosan derivative composite fibers	67:111	alginate/chitosan derivative composite fibers	67:111	Study on polysaccharide polyelectrolyte complex and fabrication of alginate/chitosan derivative composite fibers.					
34051261	1	60	theme	quaternary	149:158	arg1	PEC					224:226	PEC	224:226	PEC	224:226	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	1	60	theme	quaternary	149:158	arg1	complex					215:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex	149:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	149:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	7	61	theme	medical	1638:1644	arg1	suture					1657:1662	medical absorbable suture	1638:1662	medical absorbable suture	1638:1662	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	2	62	theme	Rotary	500:505	arg1	Rheometer					507:515	DV-C Rotary Rheometer	495:515	DV-C Rotary Rheometer	495:515	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	5	63	theme	excellent	1150:1158	arg1	performances					1160:1171	excellent performances	1150:1171	excellent performances of breaking strength 2.37 cN·dtex-1	1150:1207	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	0	64	theme	fibers	106:111	arg1	fabrication					52:62	fabrication	52:62	fabrication of alginate/chitosan derivative composite fibers	52:111	Study on polysaccharide polyelectrolyte complex and fabrication of alginate/chitosan derivative composite fibers.					
34051261	0	64	theme	fibers	106:111	arg1	complex					40:46	polysaccharide polyelectrolyte complex	9:46	polysaccharide polyelectrolyte complex	9:46	Study on polysaccharide polyelectrolyte complex and fabrication of alginate/chitosan derivative composite fibers.					
34051261	1	65	theme	chitosan	169:176	arg1	PEC					224:226	PEC	224:226	PEC	224:226	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	1	65	theme	chitosan	169:176	arg1	complex					215:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex	149:221	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	149:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	3	66	theme	turbid	818:823	arg1	solution					825:832	turbid solution	818:832	turbid solution with flocculent precipitate	818:860	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	2	67	theme	fiber	626:630	arg1	meter					641:645	YG004 single fiber strength meter	613:645	YG004 single fiber strength meter	613:645	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	1	68	theme	composite	354:362	arg1	fibers					364:369	SA/QAC composite fibers	347:369	SA/QAC composite fibers	347:369	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	3	69	dep	changed	773:779	arg1	accompanied					894:904	accompanied	894:904	accompanied by the electrical potential change	894:939	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	5	70	with	fibers	1138:1143	arg1	performances					1160:1171	excellent performances	1150:1171	excellent performances of breaking strength 2.37 cN·dtex-1	1150:1207	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	2	71	theme	YG004	613:617	arg1	meter					641:645	YG004 single fiber strength meter	613:645	YG004 single fiber strength meter	613:645	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	3	72	theme	binary	748:753	arg1	solution					755:762	the binary solution	744:762	the binary solution	744:762	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	5	73	theme	uniform	1026:1032	arg1	solution					1038:1045	this uniform PEC solution	1021:1045	this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%	1021:1238	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	1	74	with	blending	135:142	arg1	system					229:234	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	149:234	quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	149:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	7	75	theme	wound	1607:1611	arg1	dressing					1613:1620	wound dressing	1607:1620	wound dressing	1607:1620	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	5	76	theme	antibacterial	1246:1258	arg1	properties					1276:1285	good antibacterial and hydrophobic properties	1241:1285	good antibacterial and hydrophobic properties	1241:1285	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	3	77	dep	10/1	725:728	arg1	to					722:723	to	722:723	to	722:723	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	5	78	theme	breaking	1213:1220	arg1	elongation					1222:1231	breaking elongation	1213:1231	breaking elongation	1213:1231	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	0	79	theme	polyelectrolyte	24:38	arg1	complex					40:46	polysaccharide polyelectrolyte complex	9:46	polysaccharide polyelectrolyte complex	9:46	Study on polysaccharide polyelectrolyte complex and fabrication of alginate/chitosan derivative composite fibers.					
34051261	1	80	theme	anionic	282:288	arg1	polyelectrolytes					303:318	anionic and cationic polyelectrolytes	282:318	anionic and cationic polyelectrolytes	282:318	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	1	81	theme	alginate	121:128	arg1	blending					135:142	Sodium alginate (SA) blending	114:142	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system	114:234	Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers.					
34051261	2	82	theme	PEC	417:419	arg1	solution					421:428	the PEC solution	413:428	the PEC solution	413:428	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	5	83	theme	5.5 wt	1103:1108	arg1	%					1109:1109	concentration 5.5 wt%	1089:1109	concentration 5.5 wt%	1089:1109	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	7	84	theme	SA/QAC	1566:1571	arg1	fiber					1583:1587	the SA/QAC composite fiber	1562:1587	the SA/QAC composite fiber	1562:1587	Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.					
34051261	2	85	theme	Size	477:480	arg1	Analyzer					482:489	Zeta Laser Particle Size Analyzer	457:489	Zeta Laser Particle Size Analyzer	457:489	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	5	86	with	water	1114:1118	arg1	performances					1160:1171	excellent performances	1150:1171	excellent performances of breaking strength 2.37 cN·dtex-1	1150:1207	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	5	87	theme	SA	1070:1071	arg1	ratio					1061:1065	the mass ratio	1052:1065	the mass ratio of SA to QAC 10/1 and concentration 5.5 wt%	1052:1109	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	6	88	dep	formed	1383:1388	arg1	indicated					1367:1375	indicated	1367:1375	indicated	1367:1375	The FT-IR and EDS determination indicated there formed egg-box between SA and Ca2+, cross-linked network between glutaraldehyde(GA) and SA, QAC, respectively.					
34051261	2	89	theme	Laser	462:466	arg1	Analyzer					482:489	Zeta Laser Particle Size Analyzer	457:489	Zeta Laser Particle Size Analyzer	457:489	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
34051261	5	90	theme	wet-spinning	1313:1324	arg1	process					1326:1332	green wet-spinning process	1307:1332	green wet-spinning process	1307:1332	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	5	91	theme	QAC	1076:1078	arg1	10/1					1080:1083	QAC 10/1	1076:1083	QAC 10/1	1076:1083	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	5	92	theme	hydrophobic	1264:1274	arg1	properties					1276:1285	good antibacterial and hydrophobic properties	1241:1285	good antibacterial and hydrophobic properties	1241:1285	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	5	93	theme	composite	1128:1136	arg1	fibers					1138:1143	SA/QAC composite fibers	1121:1143	SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1	1121:1207	By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex-1 and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process.					
34051261	3	94	theme	potential	924:932	arg1	change					934:939	the electrical potential change	909:939	the electrical potential change	909:939	The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change.					
34051261	2	95	theme	Zeta	457:460	arg1	Analyzer					482:489	Zeta Laser Particle Size Analyzer	457:489	Zeta Laser Particle Size Analyzer	457:489	The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter.					
33143621	6	0	theme	aerogels	895:902	arg1	compositions					869:880	various compositions	861:880	various compositions of composite aerogels	861:902	In this regard, various compositions of composite aerogels have been explored by researchers to make them suitable for use in these applications.					
33143621	7	1	theme	composite	1078:1086	arg1	aerogels					1088:1095	composite aerogels	1078:1095	composite aerogels for biomedical and environmental applications	1078:1141	In the present study, an attempt has been made to briefly summarize various studies of composite aerogels for biomedical and environmental applications.					
33143621	1	2	theme	surface	251:257	arg1	chemistry					259:267	tunable surface chemistry	243:267	tunable surface chemistry	243:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	5	3	theme	ingredient	751:760	arg1	materials					762:770	the ingredient materials	747:770	the ingredient materials along with their net efficiency and cost	747:811	In designing composite aerogels for biomedical and environmental purposes, the nature of the ingredient materials along with their net efficiency and cost are important to be considered.					
33143621	5	4	theme	materials	762:770	arg1	important					817:825	important	817:825	important	817:825	In designing composite aerogels for biomedical and environmental purposes, the nature of the ingredient materials along with their net efficiency and cost are important to be considered.					
33143621	5	4	theme	materials	762:770	arg1	nature					737:742	the nature	733:742	the nature of the ingredient materials along with their net efficiency and cost	733:811	In designing composite aerogels for biomedical and environmental purposes, the nature of the ingredient materials along with their net efficiency and cost are important to be considered.					
33143621	7	5	theme	aerogels	1088:1095	arg1	studies					1067:1073	various studies	1059:1073	various studies of composite aerogels for biomedical and environmental applications	1059:1141	In the present study, an attempt has been made to briefly summarize various studies of composite aerogels for biomedical and environmental applications.					
33143621	1	6	theme	high	173:176	arg1	area					186:189	high surface area	173:189	high surface area	173:189	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	1	7	theme	chemistry	259:267	arg1	characteristics					154:168	extraordinary characteristics	140:168	extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	140:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	5	8	theme	environmental	709:721	arg1	purposes					723:730	biomedical and environmental purposes	694:730	biomedical and environmental purposes	694:730	In designing composite aerogels for biomedical and environmental purposes, the nature of the ingredient materials along with their net efficiency and cost are important to be considered.					
33143621	2	9	theme	alumina	290:296	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	3	10	theme	performance	534:544	arg1	efficiency					546:555	performance efficiency	534:555	performance efficiency	534:555	Several attempts have been made to improve the characteristics and performance efficiency of the aerogels.					
33143621	6	11	theme	various	861:867	arg1	compositions					869:880	various compositions	861:880	various compositions of composite aerogels	861:902	In this regard, various compositions of composite aerogels have been explored by researchers to make them suitable for use in these applications.					
33143621	1	12	with	density	127:133	arg1	characteristics					154:168	extraordinary characteristics	140:168	extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	140:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	5	13	theme	net	789:791	arg1	efficiency					793:802	their net efficiency	783:802	their net efficiency	783:802	In designing composite aerogels for biomedical and environmental purposes, the nature of the ingredient materials along with their net efficiency and cost are important to be considered.					
33143621	2	14	theme	silica	282:287	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	1	15	theme	surface	178:184	arg1	area					186:189	high surface area	173:189	high surface area	173:189	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	2	16	theme	potential	443:451	arg1	applications					453:464	different potential applications	433:464	different potential applications	433:464	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	4	17	theme	those	581:585	arg1	One					574:576	One	574:576	One	574:576	One of those is to fabricate composite aerogels to be used in several applications.					
33143621	4	17	theme	those	581:585	arg1	those					581:585	those	581:585	those	581:585	One of those is to fabricate composite aerogels to be used in several applications.					
33143621	1	18	theme	area	186:189	arg1	characteristics					154:168	extraordinary characteristics	140:168	extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	140:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	2	19	theme	much	415:418	arg1	interest					420:427	much interest	415:427	much interest	415:427	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	2	20	theme	metals	307:312	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	0	21	theme	Composite	0:8	arg1	Aerogels					10:17	Composite Aerogels	0:17	Composite Aerogels for Biomedical and Environmental Applications	0:63	Composite Aerogels for Biomedical and Environmental Applications.					
33143621	1	22	theme	advanced	90:97	arg1	materials					99:107	advanced materials	90:107	advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	90:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	2	23	theme	gelatin	346:352	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	0	24	theme	Biomedical	23:32	arg1	Applications					52:63	Biomedical and Environmental Applications	23:63	Biomedical and Environmental Applications	23:63	Composite Aerogels for Biomedical and Environmental Applications.					
33143621	1	25	theme	materials	99:107	arg1	Aerogels					66:73	Aerogels	66:73	Aerogels	66:73	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	1	25	theme	materials	99:107	arg1	class					81:85	a class	79:85	a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	79:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	1	26	theme	porosity	200:207	arg1	characteristics					154:168	extraordinary characteristics	140:168	extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	140:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	2	27	theme	others	393:398	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	3	28	dep	Several	467:473	arg1	attempts					475:482	attempts	475:482	attempts	475:482	Several attempts have been made to improve the characteristics and performance efficiency of the aerogels.					
33143621	2	29	theme	clay	329:332	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	4	30	theme	composite	603:611	arg1	aerogels					613:620	composite aerogels	603:620	composite aerogels	603:620	One of those is to fabricate composite aerogels to be used in several applications.					
33143621	0	31	theme	Environmental	38:50	arg1	Applications					52:63	Biomedical and Environmental Applications	23:63	Biomedical and Environmental Applications	23:63	Composite Aerogels for Biomedical and Environmental Applications.					
33143621	2	32	theme	oxides	321:326	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	5	33	theme	biomedical	694:703	arg1	purposes					723:730	biomedical and environmental purposes	694:730	biomedical and environmental purposes	694:730	In designing composite aerogels for biomedical and environmental purposes, the nature of the ingredient materials along with their net efficiency and cost are important to be considered.					
33143621	2	34	theme	polymers	375:382	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	1	35	theme	extreme	192:198	arg1	porosity					200:207	extreme porosity	192:207	extreme porosity	192:207	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	1	36	theme	lowest	210:215	arg1	conductivity					225:236	lowest thermal conductivity	210:236	lowest thermal conductivity	210:236	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	2	37	theme	different	433:441	arg1	applications					453:464	different potential applications	433:464	different potential applications	433:464	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	1	38	contain	having	109:114	arg1	materials					99:107	advanced materials	90:107	advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	90:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	1	38	contain	having	109:114	arg2	density					127:133	the lowest density	116:133	the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	116:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	7	39	theme	environmental	1116:1128	arg1	applications					1130:1141	biomedical and environmental applications	1101:1141	biomedical and environmental applications	1101:1141	In the present study, an attempt has been made to briefly summarize various studies of composite aerogels for biomedical and environmental applications.					
33143621	1	40	theme	thermal	217:223	arg1	conductivity					225:236	lowest thermal conductivity	210:236	lowest thermal conductivity	210:236	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	6	41	from	use	964:966	arg1	applications					977:988	these applications	971:988	these applications	971:988	In this regard, various compositions of composite aerogels have been explored by researchers to make them suitable for use in these applications.					
33143621	3	42	theme	aerogels	564:571	arg1	characteristics					514:528	characteristics	514:528	characteristics	514:528	Several attempts have been made to improve the characteristics and performance efficiency of the aerogels.					
33143621	3	42	theme	aerogels	564:571	arg1	efficiency					546:555	performance efficiency	534:555	performance efficiency	534:555	Several attempts have been made to improve the characteristics and performance efficiency of the aerogels.					
33143621	7	43	theme	biomedical	1101:1110	arg1	applications					1130:1141	biomedical and environmental applications	1101:1141	biomedical and environmental applications	1101:1141	In the present study, an attempt has been made to briefly summarize various studies of composite aerogels for biomedical and environmental applications.					
33143621	6	44	theme	composite	885:893	arg1	aerogels					895:902	composite aerogels	885:902	composite aerogels	885:902	In this regard, various compositions of composite aerogels have been explored by researchers to make them suitable for use in these applications.					
33143621	7	45	theme	various	1059:1065	arg1	studies					1067:1073	various studies	1059:1073	various studies of composite aerogels for biomedical and environmental applications	1059:1141	In the present study, an attempt has been made to briefly summarize various studies of composite aerogels for biomedical and environmental applications.					
33143621	4	46	used	used	628:631	arg2	those					581:585	those	581:585	those	581:585	One of those is to fabricate composite aerogels to be used in several applications.					
33143621	4	46	used	used	628:631	arg2	One					574:576	One	574:576	One	574:576	One of those is to fabricate composite aerogels to be used in several applications.					
33143621	1	47	theme	lowest	120:125	arg1	density					127:133	the lowest density	116:133	the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	116:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	7	48	theme	present	998:1004	arg1	study					1006:1010	the present study	994:1010	the present study	994:1010	In the present study, an attempt has been made to briefly summarize various studies of composite aerogels for biomedical and environmental applications.					
33143621	4	49	theme	several	636:642	arg1	applications					644:655	several applications	636:655	several applications	636:655	One of those is to fabricate composite aerogels to be used in several applications.					
33143621	2	50	theme	metal	315:319	arg1	oxides					321:326	metal oxides	315:326	metal oxides	315:326	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	2	51	theme	carbon	299:304	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	1	52	theme	conductivity	225:236	arg1	characteristics					154:168	extraordinary characteristics	140:168	extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	140:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	5	53	theme	composite	671:679	arg1	aerogels					681:688	composite aerogels	671:688	composite aerogels	671:688	In designing composite aerogels for biomedical and environmental purposes, the nature of the ingredient materials along with their net efficiency and cost are important to be considered.					
33143621	3	54	dep	characteristics	514:528	arg1	the					510:512	the	510:512	the	510:512	Several attempts have been made to improve the characteristics and performance efficiency of the aerogels.					
33143621	2	55	theme	synthetic	365:373	arg1	polymers					375:382	synthetic polymers	365:382	synthetic polymers	365:382	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	2	56	theme	many	388:391	arg1	others					393:398	many others	388:398	many others	388:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	1	57	theme	extraordinary	140:152	arg1	characteristics					154:168	extraordinary characteristics	140:168	extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry	140:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33143621	2	58	theme	chitosan	355:362	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	2	59	theme	cellulose	335:343	arg1	Aerogels					270:277	Aerogels	270:277	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others	270:398	Aerogels of silica, alumina, carbon, metals, metal oxides, clay, cellulose, gelatin, chitosan, synthetic polymers and many others have attracted much interest for different potential applications.					
33143621	1	60	theme	tunable	243:249	arg1	chemistry					259:267	tunable surface chemistry	243:267	tunable surface chemistry	243:267	Aerogels are a class of advanced materials having the lowest density with extraordinary characteristics of high surface area, extreme porosity, lowest thermal conductivity, and tunable surface chemistry.					
33910735	2	0	theme	low	354:356	arg1	efficiency					358:367	low efficiency	354:367	low efficiency	354:367	However, the conventional strategies result in poor composite effect with low efficiency.					
33910735	3	1	from	nanoparticles	515:527	arg1	matrix					544:549	BNC matrix	540:549	BNC matrix	540:549	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	1	2	theme	advantageous	238:249	arg1	properties					251:260	the advantageous properties	234:260	the advantageous properties of each material	234:277	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material.					
33910735	0	3	theme	nanocellulose	88:100	arg1	conduits					102:109	bacterial nanocellulose conduits	78:109	bacterial nanocellulose conduits	78:109	A novel approach for efficient fabrication of chitosan nanoparticles-embedded bacterial nanocellulose conduits.					
33910735	6	4	dep	adhesion	1003:1010	arg1	the					999:1001	the	999:1001	the	999:1001	The CSNPs-BNC composites could promote the adhesion and proliferation of Schwann cells, and demonstrate good biocompatibility both in vitro and in vivo.					
33910735	5	5	contain	had	880:882	arg1	composites					869:878	The CSNPs-BNC composites	855:878	The CSNPs-BNC composites	855:878	The CSNPs-BNC composites had excellent antibacterial activity to support potential clinical application.					
33910735	5	5	contain	had	880:882	arg2	activity					908:915	excellent antibacterial activity	884:915	excellent antibacterial activity	884:915	The CSNPs-BNC composites had excellent antibacterial activity to support potential clinical application.					
33910735	5	6	theme	antibacterial	894:906	arg1	activity					908:915	excellent antibacterial activity	884:915	excellent antibacterial activity	884:915	The CSNPs-BNC composites had excellent antibacterial activity to support potential clinical application.					
33910735	4	7	theme	crosslinking	752:763	arg1	agents					765:770	crosslinking agents	752:770	crosslinking agents	752:770	This composite method is simple and efficient, without introducing dispersants and crosslinking agents, while retaining the mechanical properties and native 3D network structure of BNC.					
33910735	6	8	theme	cells	1041:1045	arg1	proliferation					1016:1028	proliferation	1016:1028	proliferation	1016:1028	The CSNPs-BNC composites could promote the adhesion and proliferation of Schwann cells, and demonstrate good biocompatibility both in vitro and in vivo.					
33910735	6	8	theme	cells	1041:1045	arg1	adhesion					1003:1010	adhesion	1003:1010	adhesion	1003:1010	The CSNPs-BNC composites could promote the adhesion and proliferation of Schwann cells, and demonstrate good biocompatibility both in vitro and in vivo.					
33910735	3	9	used	utilized	436:443	arg2	network					417:423	the three-dimensional fibrillar network	385:423	the three-dimensional fibrillar network of BNC	385:430	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	3	9	used	utilized	436:443	arg2	template					450:457	a template	448:457	a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	448:666	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	5	10	theme	clinical	938:945	arg1	application					947:957	potential clinical application	928:957	potential clinical application	928:957	The CSNPs-BNC composites had excellent antibacterial activity to support potential clinical application.					
33910735	7	11	theme	promising	1164:1172	arg1	candidate					1174:1182	a promising candidate	1162:1182	a promising candidate for biomedical applications	1162:1210	The results indicated that CSNPs-BNC can provide a promising candidate for biomedical applications.					
33910735	6	12	theme	Schwann	1033:1039	arg1	cells					1041:1045	Schwann cells	1033:1045	Schwann cells	1033:1045	The CSNPs-BNC composites could promote the adhesion and proliferation of Schwann cells, and demonstrate good biocompatibility both in vitro and in vivo.					
33910735	5	13	theme	CSNPs-BNC	859:867	arg1	composites					869:878	The CSNPs-BNC composites	855:878	The CSNPs-BNC composites	855:878	The CSNPs-BNC composites had excellent antibacterial activity to support potential clinical application.					
33910735	0	14	theme	novel	2:6	arg1	approach					8:15	A novel approach	0:15	A novel approach for efficient fabrication of chitosan	0:53	A novel approach for efficient fabrication of chitosan nanoparticles-embedded bacterial nanocellulose conduits.					
33910735	3	15	theme	BNC	540:542	arg1	matrix					544:549	BNC matrix	540:549	BNC matrix	540:549	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	1	16	theme	material	270:277	arg1	properties					251:260	the advantageous properties	234:260	the advantageous properties of each material	234:277	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material.					
33910735	4	17	theme	BNC	850:852	arg1	structure					837:845	native 3D network structure	819:845	native 3D network structure	819:845	This composite method is simple and efficient, without introducing dispersants and crosslinking agents, while retaining the mechanical properties and native 3D network structure of BNC.					
33910735	4	17	theme	BNC	850:852	arg1	properties					804:813	the mechanical properties	789:813	the mechanical properties	789:813	This composite method is simple and efficient, without introducing dispersants and crosslinking agents, while retaining the mechanical properties and native 3D network structure of BNC.					
33910735	3	18	theme	BNC	428:430	arg1	template					450:457	a template	448:457	a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	448:666	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	3	18	theme	BNC	428:430	arg1	network					417:423	the three-dimensional fibrillar network	385:423	the three-dimensional fibrillar network of BNC	385:430	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	7	19	theme	biomedical	1188:1197	arg1	applications					1199:1210	biomedical applications	1188:1210	biomedical applications	1188:1210	The results indicated that CSNPs-BNC can provide a promising candidate for biomedical applications.					
33910735	4	20	theme	3D	826:827	arg1	structure					837:845	native 3D network structure	819:845	native 3D network structure	819:845	This composite method is simple and efficient, without introducing dispersants and crosslinking agents, while retaining the mechanical properties and native 3D network structure of BNC.					
33910735	2	21	theme	conventional	293:304	arg1	strategies					306:315	the conventional strategies	289:315	the conventional strategies	289:315	However, the conventional strategies result in poor composite effect with low efficiency.					
33910735	4	22	theme	native	819:824	arg1	structure					837:845	native 3D network structure	819:845	native 3D network structure	819:845	This composite method is simple and efficient, without introducing dispersants and crosslinking agents, while retaining the mechanical properties and native 3D network structure of BNC.					
33910735	1	23	theme	great	191:195	arg1	interests					197:205	great interests	191:205	great interests	191:205	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material.					
33910735	3	24	theme	nanocellulose	631:643	arg1	composites					657:666	chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	589:666	chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	589:666	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	1	25	theme	chitosan	129:136	arg1	Incorporation					112:124	Incorporation	112:124	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix	112:183	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material.					
33910735	3	26	theme	nanoparticles-embedded	598:619	arg1	composites					657:666	chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	589:666	chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	589:666	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	4	27	theme	composite	674:682	arg1	method					684:689	This composite method	669:689	This composite method	669:689	This composite method is simple and efficient, without introducing dispersants and crosslinking agents, while retaining the mechanical properties and native 3D network structure of BNC.					
33910735	3	28	theme	fibrillar	407:415	arg1	template					450:457	a template	448:457	a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	448:666	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	3	28	theme	fibrillar	407:415	arg1	network					417:423	the three-dimensional fibrillar network	385:423	the three-dimensional fibrillar network of BNC	385:430	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	5	29	theme	potential	928:936	arg1	application					947:957	potential clinical application	928:957	potential clinical application	928:957	The CSNPs-BNC composites had excellent antibacterial activity to support potential clinical application.					
33910735	3	30	theme	gelation	561:568	arg1	method					570:575	ionic gelation method	555:575	ionic gelation method	555:575	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	0	31	theme	efficient	21:29	arg1	fabrication					31:41	efficient fabrication	21:41	efficient fabrication of chitosan	21:53	A novel approach for efficient fabrication of chitosan nanoparticles-embedded bacterial nanocellulose conduits.					
33910735	2	32	with	effect	342:347	arg1	efficiency					358:367	low efficiency	354:367	low efficiency	354:367	However, the conventional strategies result in poor composite effect with low efficiency.					
33910735	1	33	theme	biomedical	210:219	arg1	field					221:225	biomedical field	210:225	biomedical field due to the advantageous properties of each material	210:277	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material.					
33910735	0	34	theme	chitosan	46:53	arg1	fabrication					31:41	efficient fabrication	21:41	efficient fabrication of chitosan	21:53	A novel approach for efficient fabrication of chitosan nanoparticles-embedded bacterial nanocellulose conduits.					
33910735	5	35	theme	excellent	884:892	arg1	activity					908:915	excellent antibacterial activity	884:915	excellent antibacterial activity	884:915	The CSNPs-BNC composites had excellent antibacterial activity to support potential clinical application.					
33910735	3	36	theme	bacterial	621:629	arg1	composites					657:666	chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	589:666	chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	589:666	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	4	37	theme	network	829:835	arg1	structure					837:845	native 3D network structure	819:845	native 3D network structure	819:845	This composite method is simple and efficient, without introducing dispersants and crosslinking agents, while retaining the mechanical properties and native 3D network structure of BNC.					
33910735	3	38	theme	three-dimensional	389:405	arg1	template					450:457	a template	448:457	a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	448:666	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	3	38	theme	three-dimensional	389:405	arg1	network					417:423	the three-dimensional fibrillar network	385:423	the three-dimensional fibrillar network of BNC	385:430	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	3	39	theme	ionic	555:559	arg1	method					570:575	ionic gelation method	555:575	ionic gelation method	555:575	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	2	40	theme	composite	332:340	arg1	effect					342:347	poor composite effect	327:347	poor composite effect with low efficiency	327:367	However, the conventional strategies result in poor composite effect with low efficiency.					
33910735	1	41	from	interests	197:205	arg1	field					221:225	biomedical field	210:225	biomedical field due to the advantageous properties of each material	210:277	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material.					
33910735	2	42	theme	poor	327:330	arg1	effect					342:347	poor composite effect	327:347	poor composite effect with low efficiency	327:367	However, the conventional strategies result in poor composite effect with low efficiency.					
33910735	3	43	theme	CSNPs-BNC	646:654	arg1	composites					657:666	chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	589:666	chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	589:666	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	3	44	theme	chitosan	589:596	arg1	composites					657:666	chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	589:666	chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	589:666	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	3	45	theme	first	467:471	arg1	time					473:476	the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	463:666	the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites	463:666	In this study, the three-dimensional fibrillar network of BNC was utilized as a template for the first time to homogeneously disperse CS to form nanoparticles (CSNPs) in BNC matrix via ionic gelation method, to develop chitosan nanoparticles-embedded bacterial nanocellulose (CSNPs-BNC) composites.					
33910735	1	46	theme	due	227:229	arg1	field					221:225	biomedical field	210:225	biomedical field due to the advantageous properties of each material	210:277	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material.					
33910735	0	47	theme	bacterial	78:86	arg1	conduits					102:109	bacterial nanocellulose conduits	78:109	bacterial nanocellulose conduits	78:109	A novel approach for efficient fabrication of chitosan nanoparticles-embedded bacterial nanocellulose conduits.					
33910735	6	48	theme	good	1064:1067	arg1	biocompatibility					1069:1084	good biocompatibility	1064:1084	good biocompatibility	1064:1084	The CSNPs-BNC composites could promote the adhesion and proliferation of Schwann cells, and demonstrate good biocompatibility both in vitro and in vivo.					
33910735	1	49	theme	Bacterial	148:156	arg1	nanocellulose					158:170	Bacterial nanocellulose	148:170	Bacterial nanocellulose (BNC) matrix	148:183	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material.					
33910735	1	49	theme	Bacterial	148:156	arg1	BNC					173:175	BNC	173:175	BNC	173:175	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material.					
33910735	4	50	theme	mechanical	793:802	arg1	properties					804:813	the mechanical properties	789:813	the mechanical properties	789:813	This composite method is simple and efficient, without introducing dispersants and crosslinking agents, while retaining the mechanical properties and native 3D network structure of BNC.					
33910735	1	51	theme	nanocellulose	158:170	arg1	matrix					178:183	Bacterial nanocellulose (BNC) matrix	148:183	Bacterial nanocellulose (BNC) matrix	148:183	Incorporation of chitosan (CS) into Bacterial nanocellulose (BNC) matrix is of great interests in biomedical field due to the advantageous properties of each material.					
33910735	6	52	theme	CSNPs-BNC	964:972	arg1	composites					974:983	The CSNPs-BNC composites	960:983	The CSNPs-BNC composites	960:983	The CSNPs-BNC composites could promote the adhesion and proliferation of Schwann cells, and demonstrate good biocompatibility both in vitro and in vivo.					
33157137	0	0	theme	degraded	106:113	arg1	polysaccharides					115:129	sulfuric acid-treated stepwise degraded polysaccharides	75:129	sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01	75:156	Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01.					
33157137	1	1	theme	study	175:179	arg1	aim					163:165	The aim	159:165	The aim of this study	159:179	The aim of this study was to investigate sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1).					
33157137	0	2	theme	stepwise	97:104	arg1	polysaccharides					115:129	sulfuric acid-treated stepwise degraded polysaccharides	75:129	sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01	75:156	Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01.					
33157137	4	3	theme	In	746:747	arg1	experiments					755:765	In vitro experiments	746:765	In vitro experiments	746:765	In vitro experiments proved that the antioxidation ability of the stepwise degraded polysaccharides gradually increased (AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4).					
33157137	5	4	theme	free	1025:1028	arg1	radicals					1030:1037	free radicals	1025:1037	free radicals in zebrafish	1025:1050	An oxidative stress zebrafish model was established, which demonstrated that the ability of AIPS-3 and AIPS-4 to scavenge free radicals in zebrafish was significantly improved compared to AIPS-1.					
33157137	6	5	from	method	1154:1159	arg1	conclusion					1102:1111	conclusion	1102:1111	conclusion	1102:1111	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	4	6	theme	degraded	821:828	arg1	polysaccharides					830:844	the stepwise degraded polysaccharides	808:844	the stepwise degraded polysaccharides	808:844	In vitro experiments proved that the antioxidation ability of the stepwise degraded polysaccharides gradually increased (AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4).					
33157137	0	7	theme	polysaccharides	115:129	arg1	characterization					55:70	structural characterization	44:70	structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01	44:156	Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01.					
33157137	4	8	theme	stepwise	812:819	arg1	polysaccharides					830:844	the stepwise degraded polysaccharides	808:844	the stepwise degraded polysaccharides	808:844	In vitro experiments proved that the antioxidation ability of the stepwise degraded polysaccharides gradually increased (AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4).					
33157137	4	9	dep	In	746:747	arg1	vitro					749:753	vitro	749:753	vitro	749:753	In vitro experiments proved that the antioxidation ability of the stepwise degraded polysaccharides gradually increased (AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4).					
33157137	3	10	theme	molar	584:588	arg1	ratio					590:594	the molar ratio	580:594	the molar ratio of monosaccharide composition	580:624	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	0	11	from	characterization	55:70	arg1	nameko					145:150	nameko	145:150	nameko	145:150	Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01.					
33157137	3	12	theme	molecular	513:521	arg1	weight					523:528	molecular weight	513:528	molecular weight	513:528	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	6	13	theme	acid	1123:1126	arg1	treatment					1128:1136	sulfuric acid treatment	1114:1136	sulfuric acid treatment	1114:1136	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	6	13	theme	acid	1123:1126	arg1	method					1154:1159	an effective method	1141:1159	an effective method for improving the antioxidant activity of polysaccharides	1141:1217	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	2	14	theme	acid	409:412	arg1	treatment					414:422	sulfuric acid treatment	400:422	sulfuric acid treatment	400:422	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	3	15	theme	Structural	425:434	arg1	characterization					436:451	Structural characterization	425:451	Structural characterization	425:451	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	2	16	theme	degraded	289:296	arg1	AIPS-3					323:328	AIPS-3	323:328	AIPS-3	323:328	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	2	16	theme	degraded	289:296	arg1	AIPS-4					335:340	AIPS-4	335:340	AIPS-4	335:340	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	2	16	theme	degraded	289:296	arg1	AIPS-2					315:320	AIPS-2	315:320	AIPS-2	315:320	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	2	16	theme	degraded	289:296	arg1	polysaccharides					298:312	Three stepwise degraded polysaccharides	274:312	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4)	274:341	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	6	17	theme	antioxidant	1179:1189	arg1	activity					1191:1198	the antioxidant activity	1175:1198	the antioxidant activity of polysaccharides	1175:1217	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	2	18	theme	sulfuric	400:407	arg1	acid					409:412	sulfuric acid	400:412	sulfuric acid treatment	400:422	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	0	19	theme	antioxidant	10:20	arg1	activity					22:29	antioxidant activity	10:29	antioxidant activity	10:29	Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01.					
33157137	3	20	theme	monosaccharide	599:612	arg1	composition					614:624	monosaccharide composition	599:624	monosaccharide composition	599:624	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	6	21	from	changes	1282:1288	arg1	characteristics					1310:1324	their structural characteristics	1293:1324	their structural characteristics	1293:1324	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	1	22	theme	sulfuric	200:207	arg1	degradation					214:224	sulfuric acid degradation	200:224	sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1)	200:271	The aim of this study was to investigate sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1).					
33157137	3	23	theme	composition	614:624	arg1	ratio					590:594	the molar ratio	580:594	the molar ratio of monosaccharide composition	580:624	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	1	24	theme	acid	209:212	arg1	degradation					214:224	sulfuric acid degradation	200:224	sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1)	200:271	The aim of this study was to investigate sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1).					
33157137	5	25	theme	oxidative	906:914	arg1	model					933:937	An oxidative stress zebrafish model	903:937	An oxidative stress zebrafish model	903:937	An oxidative stress zebrafish model was established, which demonstrated that the ability of AIPS-3 and AIPS-4 to scavenge free radicals in zebrafish was significantly improved compared to AIPS-1.					
33157137	6	26	theme	structural	1299:1308	arg1	characteristics					1310:1324	their structural characteristics	1293:1324	their structural characteristics	1293:1324	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	5	27	theme	zebrafish	923:931	arg1	model					933:937	An oxidative stress zebrafish model	903:937	An oxidative stress zebrafish model	903:937	An oxidative stress zebrafish model was established, which demonstrated that the ability of AIPS-3 and AIPS-4 to scavenge free radicals in zebrafish was significantly improved compared to AIPS-1.					
33157137	4	28	theme	antioxidation	783:795	arg1	ability					797:803	the antioxidation ability	779:803	the antioxidation ability of the stepwise degraded polysaccharides	779:844	In vitro experiments proved that the antioxidation ability of the stepwise degraded polysaccharides gradually increased (AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4).					
33157137	5	29	theme	AIPS-3	995:1000	arg1	ability					984:990	the ability	980:990	the ability of AIPS-3 and AIPS-4 to scavenge free radicals in zebrafish	980:1050	An oxidative stress zebrafish model was established, which demonstrated that the ability of AIPS-3 and AIPS-4 to scavenge free radicals in zebrafish was significantly improved compared to AIPS-1.					
33157137	3	30	theme	uronic	556:561	arg1	acid					563:566	uronic acid	556:566	uronic acid	556:566	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	0	31	theme	structural	44:53	arg1	characterization					55:70	structural characterization	44:70	structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01	44:156	Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01.					
33157137	6	32	theme	increased	1224:1232	arg1	activity					1246:1253	increased antioxidant activity	1224:1253	increased antioxidant activity	1224:1253	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	4	33	dep	increased	856:864	arg1	AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4					867:899	AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4	867:899	AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4	867:899	In vitro experiments proved that the antioxidation ability of the stepwise degraded polysaccharides gradually increased (AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4).					
33157137	3	34	theme	sulfuric	465:472	arg1	acid					474:477	sulfuric acid	465:477	sulfuric acid treatment	465:487	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	3	35	theme	triple	669:674	arg1	conformation					684:695	the triple helical conformation	665:695	the triple helical conformation of polysaccharides	665:714	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	3	36	theme	major	637:641	arg1	groups					654:659	the major functional groups	633:659	the major functional groups	633:659	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	3	36	theme	major	637:641	arg1	polysaccharides					700:714	polysaccharides	700:714	polysaccharides	700:714	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	2	37	theme	treatment	414:422	arg1	strength					388:395	the strength	384:395	the strength of sulfuric acid treatment	384:422	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	3	38	theme	helical	676:682	arg1	conformation					684:695	the triple helical conformation	665:695	the triple helical conformation of polysaccharides	665:714	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	6	39	theme	polysaccharides	1203:1217	arg1	activity					1191:1198	the antioxidant activity	1175:1198	the antioxidant activity of polysaccharides	1175:1217	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	6	40	theme	antioxidant	1234:1244	arg1	activity					1246:1253	increased antioxidant activity	1224:1253	increased antioxidant activity	1224:1253	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	0	41	from	nameko	145:150	arg1	characterization					55:70	structural characterization	44:70	structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01	44:156	Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01.					
33157137	0	41	from	nameko	145:150	arg1	polysaccharides					115:129	sulfuric acid-treated stepwise degraded polysaccharides	75:129	sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01	75:156	Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01.					
33157137	5	42	from	radicals	1030:1037	arg1	zebrafish					1042:1050	zebrafish	1042:1050	zebrafish	1042:1050	An oxidative stress zebrafish model was established, which demonstrated that the ability of AIPS-3 and AIPS-4 to scavenge free radicals in zebrafish was significantly improved compared to AIPS-1.					
33157137	5	43	theme	AIPS-4	1006:1011	arg1	ability					984:990	the ability	980:990	the ability of AIPS-3 and AIPS-4 to scavenge free radicals in zebrafish	980:1050	An oxidative stress zebrafish model was established, which demonstrated that the ability of AIPS-3 and AIPS-4 to scavenge free radicals in zebrafish was significantly improved compared to AIPS-1.					
33157137	2	44	theme	stepwise	280:287	arg1	AIPS-3					323:328	AIPS-3	323:328	AIPS-3	323:328	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	2	44	theme	stepwise	280:287	arg1	AIPS-4					335:340	AIPS-4	335:340	AIPS-4	335:340	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	2	44	theme	stepwise	280:287	arg1	AIPS-2					315:320	AIPS-2	315:320	AIPS-2	315:320	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	2	44	theme	stepwise	280:287	arg1	polysaccharides					298:312	Three stepwise degraded polysaccharides	274:312	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4)	274:341	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	6	45	theme	sulfuric	1114:1121	arg1	acid					1123:1126	sulfuric acid	1114:1126	sulfuric acid treatment	1114:1136	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	1	46	theme	nameko	242:247	arg1	AIPS-1					265:270	AIPS-1	265:270	AIPS-1	265:270	The aim of this study was to investigate sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1).					
33157137	1	46	theme	nameko	242:247	arg1	polysaccharide					249:262	the Pholiota nameko polysaccharide	229:262	the Pholiota nameko polysaccharide (AIPS-1)	229:271	The aim of this study was to investigate sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1).					
33157137	4	47	theme	polysaccharides	830:844	arg1	ability					797:803	the antioxidation ability	779:803	the antioxidation ability of the stepwise degraded polysaccharides	779:844	In vitro experiments proved that the antioxidation ability of the stepwise degraded polysaccharides gradually increased (AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4).					
33157137	3	48	theme	polysaccharides	700:714	arg1	conformation					684:695	the triple helical conformation	665:695	the triple helical conformation of polysaccharides	665:714	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	3	48	theme	polysaccharides	700:714	arg1	groups					654:659	the major functional groups	633:659	the major functional groups	633:659	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	3	48	theme	polysaccharides	700:714	arg1	polysaccharides					700:714	polysaccharides	700:714	polysaccharides	700:714	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	1	49	theme	polysaccharide	249:262	arg1	degradation					214:224	sulfuric acid degradation	200:224	sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1)	200:271	The aim of this study was to investigate sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1).					
33157137	0	50	theme	acid-treated	84:95	arg1	polysaccharides					115:129	sulfuric acid-treated stepwise degraded polysaccharides	75:129	sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01	75:156	Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01.					
33157137	1	51	theme	Pholiota	233:240	arg1	AIPS-1					265:270	AIPS-1	265:270	AIPS-1	265:270	The aim of this study was to investigate sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1).					
33157137	1	51	theme	Pholiota	233:240	arg1	polysaccharide					249:262	the Pholiota nameko polysaccharide	229:262	the Pholiota nameko polysaccharide (AIPS-1)	229:271	The aim of this study was to investigate sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1).					
33157137	3	52	theme	functional	643:652	arg1	groups					654:659	the major functional groups	633:659	the major functional groups	633:659	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	3	52	theme	functional	643:652	arg1	polysaccharides					700:714	polysaccharides	700:714	polysaccharides	700:714	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	6	53	theme	effective	1144:1152	arg1	treatment					1128:1136	sulfuric acid treatment	1114:1136	sulfuric acid treatment	1114:1136	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	6	53	theme	effective	1144:1152	arg1	method					1154:1159	an effective method	1141:1159	an effective method for improving the antioxidant activity of polysaccharides	1141:1217	In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.					
33157137	2	54	dep	polysaccharides	298:312	arg1	AIPS-3					323:328	AIPS-3	323:328	AIPS-3	323:328	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	2	54	dep	polysaccharides	298:312	arg1	AIPS-4					335:340	AIPS-4	335:340	AIPS-4	335:340	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	2	54	dep	polysaccharides	298:312	arg1	AIPS-2					315:320	AIPS-2	315:320	AIPS-2	315:320	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	2	54	dep	polysaccharides	298:312	arg1	polysaccharides					298:312	Three stepwise degraded polysaccharides	274:312	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4)	274:341	Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment.					
33157137	3	55	theme	acid	474:477	arg1	treatment					479:487	sulfuric acid treatment	465:487	sulfuric acid treatment	465:487	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
33157137	0	56	theme	sulfuric	75:82	arg1	polysaccharides					115:129	sulfuric acid-treated stepwise degraded polysaccharides	75:129	sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01	75:156	Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01.					
33157137	5	57	theme	stress	916:921	arg1	model					933:937	An oxidative stress zebrafish model	903:937	An oxidative stress zebrafish model	903:937	An oxidative stress zebrafish model was established, which demonstrated that the ability of AIPS-3 and AIPS-4 to scavenge free radicals in zebrafish was significantly improved compared to AIPS-1.					
33157137	3	58	theme	acid	563:566	arg1	content					545:551	the content	541:551	the content of uronic acid	541:566	Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly.					
32874351	9	0	theme	architecture	1702:1713	arg1	description					1675:1685	This alternative description	1658:1685	This alternative description of glycans' 3D architecture	1658:1713	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	7	1	theme	local	1431:1435	arg1	environment					1445:1455	the local spatial environment	1427:1455	the local spatial environment of groups of monosaccharides	1427:1484	Our results suggest that the N-glycans' architecture can be described in terms of the local spatial environment of groups of monosaccharides.					
32874351	6	2	theme	mammalian	1235:1243	arg1	motifs					1254:1259	mammalian N-glycan motifs	1235:1259	mammalian N-glycan motifs	1235:1259	We also compare these structural motifs and combine them with mammalian N-glycan motifs to devise strategies for the control of the N-glycan 3D structure through sequence.					
32874351	4	3	theme	undetected	868:877	arg1	part					848:851	the large part	838:851	the large part unresolved and undetected	838:877	These events are inherently linked to the N-glycans' 3D architecture and dynamics, which remain for the large part unresolved and undetected because of their intrinsic structural disorder.					
32874351	9	4	theme	glycoengineering	1856:1871	arg1	design					1873:1878	glycoengineering design	1856:1878	glycoengineering design	1856:1878	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	6	5	theme	structure	1317:1325	arg1	control					1290:1296	the control	1286:1296	the control of the N-glycan 3D structure through sequence	1286:1342	We also compare these structural motifs and combine them with mammalian N-glycan motifs to devise strategies for the control of the N-glycan 3D structure through sequence.					
32874351	5	6	theme	specific	1073:1080	arg1	modifications					1082:1094	very specific modifications	1068:1094	very specific modifications found in plants and invertebrate N-glycans	1068:1137	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	7	7	theme	spatial	1437:1443	arg1	environment					1445:1455	the local spatial environment	1427:1455	the local spatial environment of groups of monosaccharides	1427:1484	Our results suggest that the N-glycans' architecture can be described in terms of the local spatial environment of groups of monosaccharides.					
32874351	1	8	theme	proteins	244:251	arg1	modifications					227:239	the most abundant and diverse post-translational modifications	178:239	the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment	178:375	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	6	9	theme	3D	1314:1315	arg1	structure					1317:1325	the N-glycan 3D structure	1301:1325	the N-glycan 3D structure	1301:1325	We also compare these structural motifs and combine them with mammalian N-glycan motifs to devise strategies for the control of the N-glycan 3D structure through sequence.					
32874351	5	10	theme	modifications	1082:1094	arg1	set					1061:1063	a set	1059:1063	a set	1059:1063	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	5	10	theme	modifications	1082:1094	arg1	immunogenic					1150:1160	immunogenic	1150:1160	immunogenic	1150:1160	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	5	10	theme	modifications	1082:1094	arg1	modifications					1082:1094	very specific modifications	1068:1094	very specific modifications found in plants and invertebrate N-glycans	1068:1137	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	1	11	with	interactions	324:335	arg1	receptors					342:350	receptors	342:350	receptors	342:350	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	1	11	with	interactions	324:335	arg1	environment					365:375	the environment	361:375	the environment	361:375	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	0	12	theme	molecular	67:75	arg1	dynamics					77:84	molecular dynamics	67:84	molecular dynamics	67:84	How and why plants and human N-glycans are different: Insight from molecular dynamics into the "glycoblocks" architecture of complex carbohydrates.					
32874351	4	13	theme	large	842:846	arg1	part					848:851	the large part	838:851	the large part unresolved and undetected	838:877	These events are inherently linked to the N-glycans' 3D architecture and dynamics, which remain for the large part unresolved and undetected because of their intrinsic structural disorder.					
32874351	9	14	with	carbohydrates	1810:1822	arg1	implications					1840:1851	important implications	1830:1851	important implications in glycoengineering design	1830:1878	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	7	15	theme	environment	1445:1455	arg1	terms					1418:1422	terms	1418:1422	terms of the local spatial environment of groups of monosaccharides	1418:1484	Our results suggest that the N-glycans' architecture can be described in terms of the local spatial environment of groups of monosaccharides.					
32874351	9	16	theme	important	1830:1838	arg1	implications					1840:1851	important implications	1830:1851	important implications in glycoengineering design	1830:1878	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	9	17	theme	alternative	1663:1673	arg1	description					1675:1685	This alternative description	1658:1685	This alternative description of glycans' 3D architecture	1658:1713	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	8	18	theme	structural/dynamic	1629:1646	arg1	nature					1572:1577	the nature	1568:1577	the nature of the residues they comprise	1568:1607	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	8	18	theme	structural/dynamic	1629:1646	arg1	features					1648:1655	structural/dynamic features	1629:1655	structural/dynamic features	1629:1655	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	2	19	theme	different	493:501	arg1	species					503:509	different species	493:509	different species	493:509	All N-glycans share a common core from which linear or branched arms stem from, with functionalization specific to different species and to the cells' health and disease state.					
32874351	9	20	theme	relationships	1785:1797	arg1	prediction					1749:1758	an easier prediction	1739:1758	an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design	1739:1878	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	5	21	theme	molecular	947:955	arg1	simulations					971:981	molecular dynamics (MD) simulations	947:981	molecular dynamics (MD) simulations	947:981	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	2	22	theme	branched	433:440	arg1	arms					442:445	linear or branched arms	423:445	linear or branched arms	423:445	All N-glycans share a common core from which linear or branched arms stem from, with functionalization specific to different species and to the cells' health and disease state.					
32874351	5	23	theme	set	1061:1063	arg1	effects					1048:1054	the effects	1044:1054	the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans	1044:1170	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	6	24	theme	structural	1195:1204	arg1	motifs					1206:1211	these structural motifs	1189:1211	these structural motifs	1189:1211	We also compare these structural motifs and combine them with mammalian N-glycan motifs to devise strategies for the control of the N-glycan 3D structure through sequence.					
32874351	5	25	theme	MD	967:968	arg1	simulations					971:981	molecular dynamics (MD) simulations	947:981	molecular dynamics (MD) simulations	947:981	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	6	26	theme	N-glycan	1245:1252	arg1	motifs					1254:1259	mammalian N-glycan motifs	1235:1259	mammalian N-glycan motifs	1235:1259	We also compare these structural motifs and combine them with mammalian N-glycan motifs to devise strategies for the control of the N-glycan 3D structure through sequence.					
32874351	5	27	theme	invertebrate	1116:1127	arg1	N-glycans					1129:1137	invertebrate N-glycans	1116:1137	invertebrate N-glycans	1116:1137	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	5	28	theme	3D	1018:1019	arg1	structure					1021:1029	N-glycans' 3D structure	1007:1029	N-glycans' 3D structure	1007:1029	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	9	29	theme	3D	1699:1700	arg1	architecture					1702:1713	glycans' 3D architecture	1690:1713	glycans' 3D architecture	1690:1713	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	5	30	located	found	1096:1100	arg1	plants					1105:1110	plants	1105:1110	plants	1105:1110	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	5	30	located	found	1096:1100	arg2	modifications					1082:1094	very specific modifications	1068:1094	very specific modifications found in plants and invertebrate N-glycans	1068:1137	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	5	30	located	found	1096:1100	arg1	N-glycans					1129:1137	invertebrate N-glycans	1116:1137	invertebrate N-glycans	1116:1137	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	3	31	dep	able	627:630	arg1	activate					669:676	activate	669:676	to activate pathways	666:685	This diversity generates a rich collection of structures, all diversely able to trigger molecular cascades and to activate pathways, which also include adverse immunogenic responses.					
32874351	3	31	dep	able	627:630	arg1	trigger					635:641	trigger	635:641	to trigger molecular cascades	632:660	This diversity generates a rich collection of structures, all diversely able to trigger molecular cascades and to activate pathways, which also include adverse immunogenic responses.					
32874351	2	32	theme	linear	423:428	arg1	arms					442:445	linear or branched arms	423:445	linear or branched arms	423:445	All N-glycans share a common core from which linear or branched arms stem from, with functionalization specific to different species and to the cells' health and disease state.					
32874351	1	33	theme	protein	268:274	arg1	folding					276:282	protein folding	268:282	protein folding	268:282	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	9	34	theme	sequence-to-structure	1763:1783	arg1	relationships					1785:1797	sequence-to-structure relationships	1763:1797	sequence-to-structure relationships	1763:1797	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	3	35	theme	adverse	707:713	arg1	responses					727:735	adverse immunogenic responses	707:735	adverse immunogenic responses	707:735	This diversity generates a rich collection of structures, all diversely able to trigger molecular cascades and to activate pathways, which also include adverse immunogenic responses.					
32874351	5	36	from	humans	1165:1170	arg1	set					1061:1063	a set	1059:1063	a set	1059:1063	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	5	36	from	humans	1165:1170	arg1	immunogenic					1150:1160	immunogenic	1150:1160	immunogenic	1150:1160	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	5	36	from	humans	1165:1170	arg1	modifications					1082:1094	very specific modifications	1068:1094	very specific modifications found in plants and invertebrate N-glycans	1068:1137	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	8	37	theme	glycoblocks	1504:1514	arg1	units					1538:1542	self-contained 3D units	1520:1542	self-contained 3D units	1520:1542	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	8	37	theme	glycoblocks	1504:1514	arg1	"					1515:1515	these "glycoblocks"	1497:1515	these "glycoblocks"	1497:1515	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	4	38	dep	architecture	794:805	arg1	the					776:778	the	776:778	the	776:778	These events are inherently linked to the N-glycans' 3D architecture and dynamics, which remain for the large part unresolved and undetected because of their intrinsic structural disorder.					
32874351	0	39	theme	human	23:27	arg1	N-glycans					29:37	human N-glycans	23:37	human N-glycans	23:37	How and why plants and human N-glycans are different: Insight from molecular dynamics into the "glycoblocks" architecture of complex carbohydrates.					
32874351	3	40	theme	immunogenic	715:725	arg1	responses					727:735	adverse immunogenic responses	707:735	adverse immunogenic responses	707:735	This diversity generates a rich collection of structures, all diversely able to trigger molecular cascades and to activate pathways, which also include adverse immunogenic responses.					
32874351	4	41	theme	structural	906:915	arg1	disorder					917:924	their intrinsic structural disorder	890:924	their intrinsic structural disorder	890:924	These events are inherently linked to the N-glycans' 3D architecture and dynamics, which remain for the large part unresolved and undetected because of their intrinsic structural disorder.					
32874351	4	42	theme	3D	791:792	arg1	architecture					794:805	N-glycans' 3D architecture	780:805	N-glycans' 3D architecture	780:805	These events are inherently linked to the N-glycans' 3D architecture and dynamics, which remain for the large part unresolved and undetected because of their intrinsic structural disorder.					
32874351	2	43	theme	common	400:405	arg1	core					407:410	a common core	398:410	a common core from which linear or branched arms stem from	398:455	All N-glycans share a common core from which linear or branched arms stem from, with functionalization specific to different species and to the cells' health and disease state.					
32874351	1	44	theme	structural	288:297	arg1	stability					299:307	structural stability	288:307	structural stability	288:307	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	0	45	theme	"	107:107	arg1	architecture					109:120	the "glycoblocks" architecture	91:120	the "glycoblocks" architecture of complex carbohydrates	91:145	How and why plants and human N-glycans are different: Insight from molecular dynamics into the "glycoblocks" architecture of complex carbohydrates.					
32874351	1	46	theme	abundant	187:194	arg1	modifications					227:239	the most abundant and diverse post-translational modifications	178:239	the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment	178:375	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	9	47	theme	complex	1802:1808	arg1	carbohydrates					1810:1822	complex carbohydrates	1802:1822	complex carbohydrates	1802:1822	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	9	48	from	implications	1840:1851	arg1	design					1873:1878	glycoengineering design	1856:1878	glycoengineering design	1856:1878	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	0	49	theme	complex	125:131	arg1	carbohydrates					133:145	complex carbohydrates	125:145	complex carbohydrates	125:145	How and why plants and human N-glycans are different: Insight from molecular dynamics into the "glycoblocks" architecture of complex carbohydrates.					
32874351	7	50	theme	monosaccharides	1470:1484	arg1	monosaccharides					1470:1484	monosaccharides	1470:1484	monosaccharides	1470:1484	Our results suggest that the N-glycans' architecture can be described in terms of the local spatial environment of groups of monosaccharides.					
32874351	7	50	theme	monosaccharides	1470:1484	arg1	groups					1460:1465	groups	1460:1465	groups of monosaccharides	1460:1484	Our results suggest that the N-glycans' architecture can be described in terms of the local spatial environment of groups of monosaccharides.					
32874351	4	51	theme	intrinsic	896:904	arg1	disorder					917:924	their intrinsic structural disorder	890:924	their intrinsic structural disorder	890:924	These events are inherently linked to the N-glycans' 3D architecture and dynamics, which remain for the large part unresolved and undetected because of their intrinsic structural disorder.					
32874351	8	52	theme	3D	1535:1536	arg1	units					1538:1542	self-contained 3D units	1520:1542	self-contained 3D units	1520:1542	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	8	52	theme	3D	1535:1536	arg1	"					1515:1515	these "glycoblocks"	1497:1515	these "glycoblocks"	1497:1515	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	4	53	theme	unresolved	853:862	arg1	part					848:851	the large part	838:851	the large part unresolved and undetected	838:877	These events are inherently linked to the N-glycans' 3D architecture and dynamics, which remain for the large part unresolved and undetected because of their intrinsic structural disorder.					
32874351	3	54	theme	molecular	643:651	arg1	cascades					653:660	molecular cascades	643:660	molecular cascades	643:660	This diversity generates a rich collection of structures, all diversely able to trigger molecular cascades and to activate pathways, which also include adverse immunogenic responses.					
32874351	8	55	theme	residues	1586:1593	arg1	linkages					1616:1623	their linkages	1610:1623	their linkages	1610:1623	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	8	55	theme	residues	1586:1593	arg1	nature					1572:1577	the nature	1568:1577	the nature of the residues they comprise	1568:1607	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	8	55	theme	residues	1586:1593	arg1	features					1648:1655	structural/dynamic features	1629:1655	structural/dynamic features	1629:1655	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	8	56	theme	self-contained	1520:1533	arg1	units					1538:1542	self-contained 3D units	1520:1542	self-contained 3D units	1520:1542	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	8	56	theme	self-contained	1520:1533	arg1	"					1515:1515	these "glycoblocks"	1497:1515	these "glycoblocks"	1497:1515	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	2	57	theme	disease	540:546	arg1	state					548:552	disease state	540:552	disease state	540:552	All N-glycans share a common core from which linear or branched arms stem from, with functionalization specific to different species and to the cells' health and disease state.					
32874351	7	58	theme	groups	1460:1465	arg1	environment					1445:1455	the local spatial environment	1427:1455	the local spatial environment of groups of monosaccharides	1427:1484	Our results suggest that the N-glycans' architecture can be described in terms of the local spatial environment of groups of monosaccharides.					
32874351	5	59	theme	dynamics	957:964	arg1	simulations					971:981	molecular dynamics (MD) simulations	947:981	molecular dynamics (MD) simulations	947:981	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	5	60	from	immunogenic	1150:1160	arg1	humans					1165:1170	humans	1165:1170	humans	1165:1170	In this work we use molecular dynamics (MD) simulations to provide insight into N-glycans' 3D structure by analysing the effects of a set of very specific modifications found in plants and invertebrate N-glycans, which are immunogenic in humans.					
32874351	0	61	theme	carbohydrates	133:145	arg1	architecture					109:120	the "glycoblocks" architecture	91:120	the "glycoblocks" architecture of complex carbohydrates	91:145	How and why plants and human N-glycans are different: Insight from molecular dynamics into the "glycoblocks" architecture of complex carbohydrates.					
32874351	0	62	from	dynamics	77:84	arg1	Insight					54:60	Insight	54:60	Insight from molecular dynamics into the "glycoblocks" architecture of complex carbohydrates	54:145	How and why plants and human N-glycans are different: Insight from molecular dynamics into the "glycoblocks" architecture of complex carbohydrates.					
32874351	3	63	theme	structures	601:610	arg1	collection					587:596	a rich collection	580:596	a rich collection of structures, all diversely able to trigger molecular cascades and to activate pathways, which also include adverse immunogenic responses	580:735	This diversity generates a rich collection of structures, all diversely able to trigger molecular cascades and to activate pathways, which also include adverse immunogenic responses.					
32874351	9	64	from	prediction	1749:1758	arg1	carbohydrates					1810:1822	complex carbohydrates	1802:1822	complex carbohydrates	1802:1822	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	6	65	theme	N-glycan	1305:1312	arg1	structure					1317:1325	the N-glycan 3D structure	1301:1325	the N-glycan 3D structure	1301:1325	We also compare these structural motifs and combine them with mammalian N-glycan motifs to devise strategies for the control of the N-glycan 3D structure through sequence.					
32874351	1	66	theme	diverse	200:206	arg1	modifications					227:239	the most abundant and diverse post-translational modifications	178:239	the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment	178:375	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	1	67	dep	proteins	244:251	arg1	mediating					314:322	mediating	314:322	mediating interactions with receptors and with the environment	314:375	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	1	67	dep	proteins	244:251	arg1	implicated					254:263	implicated	254:263	implicated in protein folding and structural stability	254:307	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	3	68	theme	rich	582:585	arg1	collection					587:596	a rich collection	580:596	a rich collection of structures, all diversely able to trigger molecular cascades and to activate pathways, which also include adverse immunogenic responses	580:735	This diversity generates a rich collection of structures, all diversely able to trigger molecular cascades and to activate pathways, which also include adverse immunogenic responses.					
32874351	1	69	theme	post-translational	208:225	arg1	modifications					227:239	the most abundant and diverse post-translational modifications	178:239	the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment	178:375	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	2	70	theme	specific	481:488	arg1	functionalization					463:479	functionalization	463:479	functionalization specific to different species and to the cells' health and disease state	463:552	All N-glycans share a common core from which linear or branched arms stem from, with functionalization specific to different species and to the cells' health and disease state.					
32874351	0	71	dep	How	0:2	arg1	Insight					54:60	Insight	54:60	Insight from molecular dynamics into the "glycoblocks" architecture of complex carbohydrates	54:145	How and why plants and human N-glycans are different: Insight from molecular dynamics into the "glycoblocks" architecture of complex carbohydrates.					
32874351	0	71	dep	How	0:2	arg1	different					43:51	different	43:51	different	43:51	How and why plants and human N-glycans are different: Insight from molecular dynamics into the "glycoblocks" architecture of complex carbohydrates.					
32874351	8	72	dep	residues	1586:1593	arg1	they					1595:1598	they	1595:1598	they	1595:1598	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	8	72	dep	residues	1586:1593	arg1	comprise					1600:1607	comprise	1600:1607	comprise	1600:1607	We define these "glycoblocks" as self-contained 3D units, uniquely identified by the nature of the residues they comprise, their linkages and structural/dynamic features.					
32874351	9	73	theme	easier	1742:1747	arg1	prediction					1749:1758	an easier prediction	1739:1758	an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design	1739:1878	This alternative description of glycans' 3D architecture can potentially lead to an easier prediction of sequence-to-structure relationships in complex carbohydrates, with important implications in glycoengineering design.					
32874351	1	74	theme	modifications	227:239	arg1	one					171:173	one	171:173	one	171:173	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	1	74	theme	modifications	227:239	arg1	N-glycosylation					152:166	The N-glycosylation	148:166	The N-glycosylation	148:166	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
32874351	1	74	theme	modifications	227:239	arg1	modifications					227:239	the most abundant and diverse post-translational modifications	178:239	the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment	178:375	The N-glycosylation is one of the most abundant and diverse post-translational modifications of proteins, implicated in protein folding and structural stability, and mediating interactions with receptors and with the environment.					
33921340	7	0	theme	cancer	1381:1386	arg1	cells					1388:1392	lung cancer cells	1376:1392	lung cancer cells	1376:1392	Our findings indicate that the oversulfation of fucoidan promotes apoptosis of lung cancer cells and the mechanism may involve the Akt/mTOR/S6 pathway.					
33921340	6	1	theme	c	1186:1186	arg1	release					1188:1194	cytochrome c release	1175:1194	cytochrome c release	1175:1194	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	1	theme	c	1186:1186	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	2	theme	SCA	975:977	arg1	properties					961:970	The anticancer properties	946:970	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells	946:1038	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	1	3	theme	Intensive	144:152	arg1	efforts					154:160	Intensive efforts	144:160	Intensive efforts	144:160	Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer.					
33921340	8	4	theme	lung	1578:1581	arg1	cancer					1583:1588	lung cancer	1578:1588	lung cancer	1578:1588	Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer.					
33921340	1	5	theme	therapy	227:233	arg1	fields					190:195	the fields	186:195	the fields of prevention, diagnosis, and therapy of lung cancer	186:248	Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer.					
33921340	0	6	theme	In	59:60	arg1	Activity					85:92	In Vitro Anti-Lung Cancer Activity	59:92	In Vitro Anti-Lung Cancer Activity	59:92	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	6	7	theme	cytochrome	1175:1184	arg1	release					1188:1194	cytochrome c release	1175:1194	cytochrome c release	1175:1194	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	7	theme	cytochrome	1175:1184	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	3	8	from	fucoidan	468:475	arg1	activity					512:519	anti-lung cancer activity	495:519	anti-lung cancer activity	495:519	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	3	8	from	fucoidan	468:475	arg1	cascades					543:550	related signaling cascades	525:550	related signaling cascades	525:550	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	5	9	dep	infrared	883:890	arg1	FTIR					893:896	FTIR	893:896	FTIR	893:896	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	0	10	theme	Cancer	78:83	arg1	Activity					85:92	In Vitro Anti-Lung Cancer Activity	59:92	In Vitro Anti-Lung Cancer Activity	59:92	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	0	11	from	Effect	0:5	arg1	Composition					31:41	Composition	31:41	Composition	31:41	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	0	11	from	Effect	0:5	arg1	Activity					85:92	In Vitro Anti-Lung Cancer Activity	59:92	In Vitro Anti-Lung Cancer Activity	59:92	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	0	11	from	Effect	0:5	arg1	Structure					44:52	Structure	44:52	Structure	44:52	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	3	12	theme	cancer	505:510	arg1	activity					512:519	anti-lung cancer activity	495:519	anti-lung cancer activity	495:519	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	3	13	theme	related	525:531	arg1	cascades					543:550	related signaling cascades	525:550	related signaling cascades	525:550	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	1	14	theme	lung	238:241	arg1	cancer					243:248	lung cancer	238:248	lung cancer	238:248	Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer.					
33921340	0	15	theme	Anti-Lung	68:76	arg1	Activity					85:92	In Vitro Anti-Lung Cancer Activity	59:92	In Vitro Anti-Lung Cancer Activity	59:92	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	7	16	theme	fucoidan	1345:1352	arg1	oversulfation					1328:1340	the oversulfation	1324:1340	the oversulfation of fucoidan	1324:1352	Our findings indicate that the oversulfation of fucoidan promotes apoptosis of lung cancer cells and the mechanism may involve the Akt/mTOR/S6 pathway.					
33921340	4	17	theme	fucoidan	702:709	arg1	derivatives					711:721	two oversulfated fucoidan derivatives	685:721	two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2)	685:741	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	4	17	theme	fucoidan	702:709	arg1	SCA-S2					735:740	SCA-S2	735:740	SCA-S2	735:740	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	4	17	theme	fucoidan	702:709	arg1	SCA-S1					724:729	SCA-S1	724:729	SCA-S1	724:729	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	6	18	theme	caspase-3	1164:1172	arg1	expression					1150:1159	expression	1150:1159	expression of caspase-3	1150:1172	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	18	theme	caspase-3	1164:1172	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	3	19	theme	fucoidan	468:475	arg1	oversulfation					451:463	oversulfation	451:463	oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades	451:550	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	1	20	theme	cancer	243:248	arg1	diagnosis					212:220	diagnosis	212:220	diagnosis	212:220	Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer.					
33921340	1	20	theme	cancer	243:248	arg1	therapy					227:233	therapy	227:233	therapy	227:233	Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer.					
33921340	1	20	theme	cancer	243:248	arg1	prevention					200:209	prevention	200:209	prevention	200:209	Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer.					
33921340	4	21	theme	native	656:661	arg1	fucoidan					663:670	a native fucoidan	654:670	a native fucoidan	654:670	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	4	21	theme	native	656:661	arg1	fucoidan					622:629	a previously developed fucoidan	599:629	a previously developed fucoidan (SCA)	599:635	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	6	22	theme	human	1007:1011	arg1	cells					1034:1038	human lung carcinoma A-549 cells	1007:1038	human lung carcinoma A-549 cells	1007:1038	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	3	23	from	effect	485:490	arg1	activity					512:519	anti-lung cancer activity	495:519	anti-lung cancer activity	495:519	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	3	23	from	effect	485:490	arg1	cascades					543:550	related signaling cascades	525:550	related signaling cascades	525:550	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	5	24	theme	nuclear	903:909	arg1	NMR					931:933	NMR	931:933	NMR	931:933	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	5	24	theme	nuclear	903:909	arg1	resonance					920:928	nuclear magnetic resonance	903:928	nuclear magnetic resonance (NMR) analyses	903:943	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	2	25	theme	sulfation	356:364	arg1	pattern					366:372	sulfation pattern	356:372	sulfation pattern	356:372	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	8	26	theme	oversulfated	1527:1538	arg1	fucoidan					1540:1547	oversulfated fucoidan	1527:1547	oversulfated fucoidan	1527:1547	Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer.					
33921340	6	27	theme	lung	1013:1016	arg1	cells					1034:1038	human lung carcinoma A-549 cells	1007:1038	human lung carcinoma A-549 cells	1007:1038	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	3	28	theme	anti-lung	495:503	arg1	activity					512:519	anti-lung cancer activity	495:519	anti-lung cancer activity	495:519	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	7	29	theme	cells	1388:1392	arg1	apoptosis					1363:1371	apoptosis	1363:1371	apoptosis of lung cancer cells	1363:1392	Our findings indicate that the oversulfation of fucoidan promotes apoptosis of lung cancer cells and the mechanism may involve the Akt/mTOR/S6 pathway.					
33921340	6	30	theme	cytotoxicity	1066:1077	arg1	expression					1150:1159	expression	1150:1159	expression of caspase-3	1150:1172	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	30	theme	cytotoxicity	1066:1077	arg1	release					1188:1194	cytochrome c release	1175:1194	cytochrome c release	1175:1194	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	30	theme	cytotoxicity	1066:1077	arg1	cycle					1085:1089	cell cycle	1080:1089	cell cycle	1080:1089	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	30	theme	cytotoxicity	1066:1077	arg1	cascades					1287:1294	the underlying signaling cascades	1262:1294	the underlying signaling cascades	1262:1294	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	30	theme	cytotoxicity	1066:1077	arg1	potential					1133:1141	mitochondrial membrane potential	1110:1141	mitochondrial membrane potential (MMP)	1110:1147	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	30	theme	cytotoxicity	1066:1077	arg1	staining					1229:1236	Annexin V/propidium iodide (PI) staining	1197:1236	Annexin V/propidium iodide (PI) staining	1197:1236	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	30	theme	cytotoxicity	1066:1077	arg1	terms					1057:1061	terms	1057:1061	terms of cytotoxicity	1057:1077	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	30	theme	cytotoxicity	1066:1077	arg1	fragmentation					1243:1255	DNA fragmentation	1239:1255	DNA fragmentation	1239:1255	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	30	theme	cytotoxicity	1066:1077	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	2	31	theme	biological	285:294	arg1	activities					296:305	biological activities	285:305	biological activities	285:305	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	2	32	theme	sulfation	345:353	arg1	weight					410:415	molecular weight	400:415	molecular weight of fucoidan	400:427	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	2	32	theme	sulfation	345:353	arg1	branches					386:393	glycosidic branches	375:393	glycosidic branches	375:393	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	2	32	theme	sulfation	345:353	arg1	pattern					366:372	sulfation pattern	356:372	sulfation pattern	356:372	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	2	32	theme	sulfation	345:353	arg1	degree					335:340	the degree	331:340	the degree of sulfation	331:353	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	2	33	theme	molecular	400:408	arg1	weight					410:415	molecular weight	400:415	molecular weight of fucoidan	400:427	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	3	34	from	oversulfation	451:463	arg1	activity					512:519	anti-lung cancer activity	495:519	anti-lung cancer activity	495:519	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	3	34	from	oversulfation	451:463	arg1	cascades					543:550	related signaling cascades	525:550	related signaling cascades	525:550	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	0	35	dep	In	59:60	arg1	Vitro					62:66	Vitro	62:66	Vitro	62:66	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	6	36	theme	PI	1225:1226	arg1	staining					1229:1236	Annexin V/propidium iodide (PI) staining	1197:1236	Annexin V/propidium iodide (PI) staining	1197:1236	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	36	theme	PI	1225:1226	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	37	theme	SCA-S1	980:985	arg1	properties					961:970	The anticancer properties	946:970	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells	946:1038	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	5	38	theme	structural	830:839	arg1	features					841:848	the characteristic structural features	811:848	the characteristic structural features of fucoidan	811:860	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	4	39	theme	oversulfated	689:700	arg1	derivatives					711:721	two oversulfated fucoidan derivatives	685:721	two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2)	685:741	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	4	39	theme	oversulfated	689:700	arg1	SCA-S2					735:740	SCA-S2	735:740	SCA-S2	735:740	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	4	39	theme	oversulfated	689:700	arg1	SCA-S1					724:729	SCA-S1	724:729	SCA-S1	724:729	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	5	40	contain	had	807:809	arg2	features					841:848	the characteristic structural features	811:848	the characteristic structural features of fucoidan	811:860	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	5	40	contain	had	807:809	arg1	SCA					744:746	SCA	744:746	SCA	744:746	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	0	41	theme	Oversulfation	10:22	arg1	Effect					0:5	Effect	0:5	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans	0:105	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	8	42	theme	in	1457:1458	arg1	research					1465:1472	Further in vivo research	1449:1472	Further in vivo research	1449:1472	Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer.					
33921340	0	43	theme	Fucoidans	97:105	arg1	Composition					31:41	Composition	31:41	Composition	31:41	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	0	43	theme	Fucoidans	97:105	arg1	Activity					85:92	In Vitro Anti-Lung Cancer Activity	59:92	In Vitro Anti-Lung Cancer Activity	59:92	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	0	43	theme	Fucoidans	97:105	arg1	Structure					44:52	Structure	44:52	Structure	44:52	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	2	44	theme	wide	271:274	arg1	range					276:280	a wide range	269:280	a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan	269:427	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	2	45	theme	glycosidic	375:384	arg1	branches					386:393	glycosidic branches	375:393	glycosidic branches	375:393	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	8	46	theme	Further	1449:1455	arg1	research					1465:1472	Further in vivo research	1449:1472	Further in vivo research	1449:1472	Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer.					
33921340	7	47	theme	Akt/mTOR/S6	1428:1438	arg1	pathway					1440:1446	the Akt/mTOR/S6 pathway	1424:1446	the Akt/mTOR/S6 pathway	1424:1446	Our findings indicate that the oversulfation of fucoidan promotes apoptosis of lung cancer cells and the mechanism may involve the Akt/mTOR/S6 pathway.					
33921340	4	48	dep	derivatives	711:721	arg1	derivatives					711:721	two oversulfated fucoidan derivatives	685:721	two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2)	685:741	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	4	48	dep	derivatives	711:721	arg1	SCA-S2					735:740	SCA-S2	735:740	SCA-S2	735:740	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	4	48	dep	derivatives	711:721	arg1	SCA-S1					724:729	SCA-S1	724:729	SCA-S1	724:729	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	4	49	theme	developed	612:620	arg1	SCA					632:634	SCA	632:634	SCA	632:634	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	4	49	theme	developed	612:620	arg1	fucoidan					663:670	a native fucoidan	654:670	a native fucoidan	654:670	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	4	49	theme	developed	612:620	arg1	fucoidan					622:629	a previously developed fucoidan	599:629	a previously developed fucoidan (SCA)	599:635	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	6	50	theme	SCA-S2	992:997	arg1	properties					961:970	The anticancer properties	946:970	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells	946:1038	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	51	theme	iodide	1217:1222	arg1	staining					1229:1236	Annexin V/propidium iodide (PI) staining	1197:1236	Annexin V/propidium iodide (PI) staining	1197:1236	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	51	theme	iodide	1217:1222	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	1	52	theme	prevention	200:209	arg1	fields					190:195	the fields	186:195	the fields of prevention, diagnosis, and therapy of lung cancer	186:248	Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer.					
33921340	2	53	theme	fucoidan	420:427	arg1	weight					410:415	molecular weight	400:415	molecular weight of fucoidan	400:427	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	2	53	theme	fucoidan	420:427	arg1	branches					386:393	glycosidic branches	375:393	glycosidic branches	375:393	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	2	53	theme	fucoidan	420:427	arg1	pattern					366:372	sulfation pattern	356:372	sulfation pattern	356:372	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	2	53	theme	fucoidan	420:427	arg1	degree					335:340	the degree	331:340	the degree of sulfation	331:353	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	6	54	theme	V/propidium	1205:1215	arg1	staining					1229:1236	Annexin V/propidium iodide (PI) staining	1197:1236	Annexin V/propidium iodide (PI) staining	1197:1236	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	54	theme	V/propidium	1205:1215	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	0	55	dep	Composition	31:41	arg1	the					27:29	the	27:29	the	27:29	Effect of Oversulfation on the Composition, Structure, and In Vitro Anti-Lung Cancer Activity of Fucoidans Extracted from Sargassum aquifolium.					
33921340	5	56	from	differences	775:785	arg1	compositions					790:801	compositions	790:801	compositions	790:801	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	3	57	theme	oversulfation	451:463	arg1	determination					434:446	The determination	430:446	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades	430:550	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	6	58	theme	signaling	1277:1285	arg1	cascades					1287:1294	the underlying signaling cascades	1262:1294	the underlying signaling cascades	1262:1294	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	59	theme	Annexin	1197:1203	arg1	staining					1229:1236	Annexin V/propidium iodide (PI) staining	1197:1236	Annexin V/propidium iodide (PI) staining	1197:1236	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	59	theme	Annexin	1197:1203	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	8	60	dep	in	1457:1458	arg1	vivo					1460:1463	vivo	1460:1463	vivo	1460:1463	Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer.					
33921340	6	61	theme	membrane	1124:1131	arg1	potential					1133:1141	mitochondrial membrane potential	1110:1141	mitochondrial membrane potential (MMP)	1110:1147	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	61	theme	membrane	1124:1131	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	61	theme	membrane	1124:1131	arg1	MMP					1144:1146	MMP	1144:1146	MMP	1144:1146	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	5	62	theme	fucoidan	853:860	arg1	features					841:848	the characteristic structural features	811:848	the characteristic structural features of fucoidan	811:860	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	3	63	theme	signaling	533:541	arg1	cascades					543:550	related signaling cascades	525:550	related signaling cascades	525:550	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	6	64	theme	cell	1080:1083	arg1	cycle					1085:1089	cell cycle	1080:1089	cell cycle	1080:1089	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	5	65	theme	resonance	920:928	arg1	analyses					936:943	nuclear magnetic resonance (NMR) analyses	903:943	nuclear magnetic resonance (NMR) analyses	903:943	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	6	66	theme	A-549	1028:1032	arg1	cells					1034:1038	human lung carcinoma A-549 cells	1007:1038	human lung carcinoma A-549 cells	1007:1038	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	67	theme	anticancer	950:959	arg1	properties					961:970	The anticancer properties	946:970	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells	946:1038	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	5	68	theme	magnetic	911:918	arg1	NMR					931:933	NMR	931:933	NMR	931:933	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	5	68	theme	magnetic	911:918	arg1	resonance					920:928	nuclear magnetic resonance	903:928	nuclear magnetic resonance (NMR) analyses	903:943	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	6	69	theme	mitochondrial	1110:1122	arg1	potential					1133:1141	mitochondrial membrane potential	1110:1141	mitochondrial membrane potential (MMP)	1110:1147	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	69	theme	mitochondrial	1110:1122	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	69	theme	mitochondrial	1110:1122	arg1	MMP					1144:1146	MMP	1144:1146	MMP	1144:1146	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	2	70	theme	activities	296:305	arg1	range					276:280	a wide range	269:280	a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan	269:427	Fucoidans exhibit a wide range of biological activities, which are dependent on the degree of sulfation, sulfation pattern, glycosidic branches, and molecular weight of fucoidan.					
33921340	5	71	dep	Fourier	865:871	arg1	transform					873:881	transform	873:881	transform infrared (FTIR)	873:897	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	1	72	theme	diagnosis	212:220	arg1	fields					190:195	the fields	186:195	the fields of prevention, diagnosis, and therapy of lung cancer	186:248	Intensive efforts have been undertaken in the fields of prevention, diagnosis, and therapy of lung cancer.					
33921340	6	73	theme	underlying	1266:1275	arg1	cascades					1287:1294	the underlying signaling cascades	1262:1294	the underlying signaling cascades	1262:1294	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	3	74	from	activity	512:519	arg1	oversulfation					451:463	oversulfation	451:463	oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades	451:550	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	5	75	theme	characteristic	815:828	arg1	features					841:848	the characteristic structural features	811:848	the characteristic structural features of fucoidan	811:860	SCA, SCA-S1, and SCA-S2 showed differences in compositions and had the characteristic structural features of fucoidan by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses.					
33921340	3	76	from	cascades	543:550	arg1	oversulfation					451:463	oversulfation	451:463	oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades	451:550	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	8	77	theme	precise	1501:1507	arg1	mechanism					1509:1517	the precise mechanism	1497:1517	the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer	1497:1588	Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer.					
33921340	6	78	theme	carcinoma	1018:1026	arg1	cells					1034:1038	human lung carcinoma A-549 cells	1007:1038	human lung carcinoma A-549 cells	1007:1038	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	7	79	theme	lung	1376:1379	arg1	cells					1388:1392	lung cancer cells	1376:1392	lung cancer cells	1376:1392	Our findings indicate that the oversulfation of fucoidan promotes apoptosis of lung cancer cells and the mechanism may involve the Akt/mTOR/S6 pathway.					
33921340	6	80	theme	Bcl-2	1092:1096	arg1	expression					1150:1159	expression	1150:1159	expression of caspase-3	1150:1172	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	80	theme	Bcl-2	1092:1096	arg1	release					1188:1194	cytochrome c release	1175:1194	cytochrome c release	1175:1194	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	80	theme	Bcl-2	1092:1096	arg1	potential					1133:1141	mitochondrial membrane potential	1110:1141	mitochondrial membrane potential (MMP)	1110:1147	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	80	theme	Bcl-2	1092:1096	arg1	staining					1229:1236	Annexin V/propidium iodide (PI) staining	1197:1236	Annexin V/propidium iodide (PI) staining	1197:1236	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	80	theme	Bcl-2	1092:1096	arg1	fragmentation					1243:1255	DNA fragmentation	1239:1255	DNA fragmentation	1239:1255	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	80	theme	Bcl-2	1092:1096	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	4	81	used	used	594:597	arg2	we					591:592	we	591:592	we	591:592	In this investigation, we used a previously developed fucoidan (SCA), which served as a native fucoidan, to generate two oversulfated fucoidan derivatives (SCA-S1 and SCA-S2).					
33921340	3	82	theme	effect	485:490	arg1	oversulfation					451:463	oversulfation	451:463	oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades	451:550	The determination of oversulfation of fucoidan and its effect on anti-lung cancer activity and related signaling cascades is challenging.					
33921340	8	83	theme	cancer	1583:1588	arg1	progression					1563:1573	the progression	1559:1573	the progression of lung cancer	1559:1588	Further in vivo research is needed to establish the precise mechanism whereby oversulfated fucoidan mitigates the progression of lung cancer.					
33921340	6	84	theme	DNA	1239:1241	arg1	fragmentation					1243:1255	DNA fragmentation	1239:1255	DNA fragmentation	1239:1255	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
33921340	6	84	theme	DNA	1239:1241	arg1	expression					1098:1107	Bcl-2 expression	1092:1107	Bcl-2 expression	1092:1107	The anticancer properties of SCA, SCA-S1, and SCA-S2 against human lung carcinoma A-549 cells were analyzed in terms of cytotoxicity, cell cycle, Bcl-2 expression, mitochondrial membrane potential (MMP), expression of caspase-3, cytochrome c release, Annexin V/propidium iodide (PI) staining, DNA fragmentation, and the underlying signaling cascades.					
32299577	2	0	theme	saliva-gastrointestinal	459:481	arg1	digestion					483:491	saliva-gastrointestinal digestion	459:491	saliva-gastrointestinal digestion (SGID)	459:498	Results showed that the digestibilities of OPS were about 5.1%, 37.5%, and 41.3% after saliva digestion (SD), saliva-gastric digestion (SGD), and saliva-gastrointestinal digestion (SGID), respectively.					
32299577	2	0	theme	saliva-gastrointestinal	459:481	arg1	SGID					494:497	SGID	494:497	SGID	494:497	Results showed that the digestibilities of OPS were about 5.1%, 37.5%, and 41.3% after saliva digestion (SD), saliva-gastric digestion (SGD), and saliva-gastrointestinal digestion (SGID), respectively.					
32299577	3	1	theme	molecular	687:695	arg1	Mw					706:707	Mw	706:707	Mw	706:707	The SGID significantly changed the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw).					
32299577	3	1	theme	molecular	687:695	arg1	weights					697:703	molecular weights	687:703	molecular weights (Mw)	687:708	The SGID significantly changed the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw).					
32299577	1	2	theme	polysaccharides	290:304	arg1	bioactivities					268:280	bioactivities	268:280	bioactivities	268:280	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	1	2	theme	polysaccharides	290:304	arg1	properties					253:262	physicochemical properties	237:262	physicochemical properties	237:262	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	1	3	theme	in	177:178	arg1	digestion					220:228	in vitro simulated saliva-gastrointestinal digestion	177:228	in vitro simulated saliva-gastrointestinal digestion	177:228	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	0	4	theme	polysaccharides	115:129	arg1	bioactivities					93:105	bioactivities	93:105	bioactivities	93:105	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides.					
32299577	0	4	theme	polysaccharides	115:129	arg1	properties					78:87	physicochemical properties	62:87	physicochemical properties	62:87	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides.					
32299577	0	5	from	Effects	0:6	arg1	bioactivities					93:105	bioactivities	93:105	bioactivities	93:105	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides.					
32299577	0	5	from	Effects	0:6	arg1	properties					78:87	physicochemical properties	62:87	physicochemical properties	62:87	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides.					
32299577	0	6	theme	okra	110:113	arg1	polysaccharides					115:129	okra polysaccharides	110:129	okra polysaccharides	110:129	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides.					
32299577	7	7	theme	OPS	1206:1208	arg1	administration					1188:1201	the oral administration	1179:1201	the oral administration of OPS	1179:1208	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	7	8	theme	valuable	1144:1151	arg1	support					1168:1174	valuable and scientific support	1144:1174	valuable and scientific support on the oral administration of OPS	1144:1208	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	7	8	theme	valuable	1144:1151	arg1	medicines					1234:1242	medicines	1234:1242	medicines	1234:1242	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	7	8	theme	valuable	1144:1151	arg1	foods					1224:1228	functional foods	1213:1228	functional foods	1213:1228	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	1	9	from	effects	166:172	arg1	bioactivities					268:280	bioactivities	268:280	bioactivities	268:280	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	1	9	from	effects	166:172	arg1	properties					253:262	physicochemical properties	237:262	physicochemical properties	237:262	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	6	10	contain	possessed	963:971	arg2	activities					1038:1047	prebiotic activities	1028:1047	prebiotic activities	1028:1047	After SGID, OPS still possessed strong antioxidant activities, binding capacities, and prebiotic activities, but the α-glucosidase inhibitory effect was obviously decreased.					
32299577	6	10	contain	possessed	963:971	arg2	activities					992:1001	strong antioxidant activities	973:1001	strong antioxidant activities	973:1001	After SGID, OPS still possessed strong antioxidant activities, binding capacities, and prebiotic activities, but the α-glucosidase inhibitory effect was obviously decreased.					
32299577	6	10	contain	possessed	963:971	arg1	OPS					953:955	OPS	953:955	OPS	953:955	After SGID, OPS still possessed strong antioxidant activities, binding capacities, and prebiotic activities, but the α-glucosidase inhibitory effect was obviously decreased.					
32299577	6	10	contain	possessed	963:971	arg2	capacities					1012:1021	binding capacities	1004:1021	binding capacities	1004:1021	After SGID, OPS still possessed strong antioxidant activities, binding capacities, and prebiotic activities, but the α-glucosidase inhibitory effect was obviously decreased.					
32299577	7	11	from	support	1168:1174	arg1	administration					1188:1201	the oral administration	1179:1201	the oral administration of OPS	1179:1208	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	6	12	theme	prebiotic	1028:1036	arg1	activities					1038:1047	prebiotic activities	1028:1047	prebiotic activities	1028:1047	After SGID, OPS still possessed strong antioxidant activities, binding capacities, and prebiotic activities, but the α-glucosidase inhibitory effect was obviously decreased.					
32299577	1	13	theme	simulated	186:194	arg1	digestion					220:228	in vitro simulated saliva-gastrointestinal digestion	177:228	in vitro simulated saliva-gastrointestinal digestion	177:228	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	4	14	theme	OPS	815:817	arg1	degradation					800:810	the degradation	796:810	the degradation of OPS during SGID	796:829	Especially, Mw changes resulted in the breakdown of glycosidic bonds during SGD, and the degradation of OPS during SGID was mainly caused by disrupting aggregates.					
32299577	2	15	theme	OPS	356:358	arg1	digestibilities					337:351	the digestibilities	333:351	the digestibilities of OPS	333:358	Results showed that the digestibilities of OPS were about 5.1%, 37.5%, and 41.3% after saliva digestion (SD), saliva-gastric digestion (SGD), and saliva-gastrointestinal digestion (SGID), respectively.					
32299577	2	15	theme	OPS	356:358	arg1	%					374:374	about 5.1%	365:374	about 5.1%	365:374	Results showed that the digestibilities of OPS were about 5.1%, 37.5%, and 41.3% after saliva digestion (SD), saliva-gastric digestion (SGD), and saliva-gastrointestinal digestion (SGID), respectively.					
32299577	1	16	theme	saliva-gastrointestinal	196:218	arg1	digestion					220:228	in vitro simulated saliva-gastrointestinal digestion	177:228	in vitro simulated saliva-gastrointestinal digestion	177:228	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	2	17	theme	saliva-gastric	423:436	arg1	SGD					449:451	SGD	449:451	SGD	449:451	Results showed that the digestibilities of OPS were about 5.1%, 37.5%, and 41.3% after saliva digestion (SD), saliva-gastric digestion (SGD), and saliva-gastrointestinal digestion (SGID), respectively.					
32299577	2	17	theme	saliva-gastric	423:436	arg1	digestion					438:446	saliva-gastric digestion	423:446	saliva-gastric digestion (SGD)	423:452	Results showed that the digestibilities of OPS were about 5.1%, 37.5%, and 41.3% after saliva digestion (SD), saliva-gastric digestion (SGD), and saliva-gastrointestinal digestion (SGID), respectively.					
32299577	1	18	dep	in	177:178	arg1	vitro					180:184	vitro	180:184	vitro	180:184	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	1	19	theme	digestion	220:228	arg1	effects					166:172	the effects	162:172	the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS)	162:310	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	0	20	theme	saliva-gastrointestinal	21:43	arg1	digestion					45:53	simulated saliva-gastrointestinal digestion	11:53	simulated saliva-gastrointestinal digestion	11:53	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides.					
32299577	4	21	theme	Mw	723:724	arg1	changes					726:732	Mw changes	723:732	Mw changes	723:732	Especially, Mw changes resulted in the breakdown of glycosidic bonds during SGD, and the degradation of OPS during SGID was mainly caused by disrupting aggregates.					
32299577	6	22	theme	binding	1004:1010	arg1	capacities					1012:1021	binding capacities	1004:1021	binding capacities	1004:1021	After SGID, OPS still possessed strong antioxidant activities, binding capacities, and prebiotic activities, but the α-glucosidase inhibitory effect was obviously decreased.					
32299577	0	23	theme	simulated	11:19	arg1	digestion					45:53	simulated saliva-gastrointestinal digestion	11:53	simulated saliva-gastrointestinal digestion	11:53	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides.					
32299577	0	24	dep	properties	78:87	arg1	the					58:60	the	58:60	the	58:60	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides.					
32299577	4	25	theme	glycosidic	763:772	arg1	bonds					774:778	glycosidic bonds	763:778	glycosidic bonds	763:778	Especially, Mw changes resulted in the breakdown of glycosidic bonds during SGD, and the degradation of OPS during SGID was mainly caused by disrupting aggregates.					
32299577	1	26	theme	physicochemical	237:251	arg1	properties					253:262	physicochemical properties	237:262	physicochemical properties	237:262	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	0	27	theme	digestion	45:53	arg1	Effects					0:6	Effects	0:6	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides	0:129	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides.					
32299577	2	28	theme	saliva	400:405	arg1	digestion					407:415	saliva digestion	400:415	saliva digestion (SD)	400:420	Results showed that the digestibilities of OPS were about 5.1%, 37.5%, and 41.3% after saliva digestion (SD), saliva-gastric digestion (SGD), and saliva-gastrointestinal digestion (SGID), respectively.					
32299577	2	28	theme	saliva	400:405	arg1	SD					418:419	SD	418:419	SD	418:419	Results showed that the digestibilities of OPS were about 5.1%, 37.5%, and 41.3% after saliva digestion (SD), saliva-gastric digestion (SGD), and saliva-gastrointestinal digestion (SGID), respectively.					
32299577	0	29	theme	physicochemical	62:76	arg1	properties					78:87	physicochemical properties	62:87	physicochemical properties	62:87	Effects of simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides.					
32299577	7	30	theme	scientific	1157:1166	arg1	support					1168:1174	valuable and scientific support	1144:1174	valuable and scientific support on the oral administration of OPS	1144:1208	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	7	30	theme	scientific	1157:1166	arg1	medicines					1234:1242	medicines	1234:1242	medicines	1234:1242	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	7	30	theme	scientific	1157:1166	arg1	foods					1224:1228	functional foods	1213:1228	functional foods	1213:1228	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	3	31	theme	total	613:617	arg1	flavonoids					619:628	total flavonoids	613:628	total flavonoids	613:628	The SGID significantly changed the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw).					
32299577	3	32	theme	OPS	580:582	arg1	properties					566:575	the physicochemical properties	546:575	the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw)	546:708	The SGID significantly changed the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw).					
32299577	3	33	theme	physicochemical	550:564	arg1	properties					566:575	the physicochemical properties	546:575	the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw)	546:708	The SGID significantly changed the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw).					
32299577	3	34	theme	rheological	659:669	arg1	properties					671:680	rheological properties	659:680	rheological properties	659:680	The SGID significantly changed the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw).					
32299577	3	35	theme	uronic	599:604	arg1	acids					606:610	total uronic acids	593:610	total uronic acids	593:610	The SGID significantly changed the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw).					
32299577	6	36	theme	inhibitory	1072:1081	arg1	effect					1083:1088	the α-glucosidase inhibitory effect	1054:1088	the α-glucosidase inhibitory effect	1054:1088	After SGID, OPS still possessed strong antioxidant activities, binding capacities, and prebiotic activities, but the α-glucosidase inhibitory effect was obviously decreased.					
32299577	5	37	theme	OPS	909:911	arg1	bioactivities					892:904	the bioactivities	888:904	the bioactivities of OPS	888:911	Furthermore, the bioactivities of OPS were also affected by SGID.					
32299577	6	38	theme	strong	973:978	arg1	activities					992:1001	strong antioxidant activities	973:1001	strong antioxidant activities	973:1001	After SGID, OPS still possessed strong antioxidant activities, binding capacities, and prebiotic activities, but the α-glucosidase inhibitory effect was obviously decreased.					
32299577	3	39	theme	monosaccharide	631:644	arg1	composition					646:656	monosaccharide composition	631:656	monosaccharide composition	631:656	The SGID significantly changed the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw).					
32299577	1	40	dep	properties	253:262	arg1	the					233:235	the	233:235	the	233:235	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	6	41	theme	α-glucosidase	1058:1070	arg1	effect					1083:1088	the α-glucosidase inhibitory effect	1054:1088	the α-glucosidase inhibitory effect	1054:1088	After SGID, OPS still possessed strong antioxidant activities, binding capacities, and prebiotic activities, but the α-glucosidase inhibitory effect was obviously decreased.					
32299577	7	42	theme	oral	1183:1186	arg1	administration					1188:1201	the oral administration	1179:1201	the oral administration of OPS	1179:1208	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	6	43	theme	antioxidant	980:990	arg1	activities					992:1001	strong antioxidant activities	973:1001	strong antioxidant activities	973:1001	After SGID, OPS still possessed strong antioxidant activities, binding capacities, and prebiotic activities, but the α-glucosidase inhibitory effect was obviously decreased.					
32299577	4	44	theme	bonds	774:778	arg1	breakdown					750:758	the breakdown	746:758	the breakdown of glycosidic bonds during SGD	746:789	Especially, Mw changes resulted in the breakdown of glycosidic bonds during SGD, and the degradation of OPS during SGID was mainly caused by disrupting aggregates.					
32299577	3	45	theme	total	593:597	arg1	acids					606:610	total uronic acids	593:610	total uronic acids	593:610	The SGID significantly changed the physicochemical properties of OPS, such as total uronic acids, total flavonoids, monosaccharide composition, rheological properties, and molecular weights (Mw).					
32299577	7	46	theme	functional	1213:1222	arg1	support					1168:1174	valuable and scientific support	1144:1174	valuable and scientific support on the oral administration of OPS	1144:1208	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	7	46	theme	functional	1213:1222	arg1	foods					1224:1228	functional foods	1213:1228	functional foods	1213:1228	Overall, results can provide valuable and scientific support on the oral administration of OPS as functional foods and medicines in the future.					
32299577	1	47	theme	okra	285:288	arg1	OPS					307:309	OPS	307:309	OPS	307:309	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32299577	1	47	theme	okra	285:288	arg1	polysaccharides					290:304	okra polysaccharides	285:304	okra polysaccharides (OPS)	285:310	This study was to investigate the effects of in vitro simulated saliva-gastrointestinal digestion on the physicochemical properties and bioactivities of okra polysaccharides (OPS).					
32004605	6	0	theme	xenograft	1203:1211	arg1	growth					1219:1224	A549 xenograft tumor growth	1198:1224	A549 xenograft tumor growth	1198:1224	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	4	1	theme	β-D-glucuronic	853:866	arg1	GlcA					874:877	GlcA	874:877	GlcA	874:877	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	1	theme	β-D-glucuronic	853:866	arg1	acid					868:871	1,4-linked β-D-glucuronic acid	842:871	1,4-linked β-D-glucuronic acid (GlcA)	842:878	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	8	2	theme	potential	1423:1431	arg1	therapeutics					1437:1448	a potential new therapeutics	1421:1448	a potential new therapeutics for the treatment of lung cancer	1421:1481	than HFSGF-L, indicating that HFSGF-H might be a leading compound for a potential new therapeutics for the treatment of lung cancer.					
32004605	4	3	from	galactofucan	616:627	arg1	HFSGF-L					569:575	HFSGF-L	569:575	HFSGF-L	569:575	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	3	from	galactofucan	616:627	arg1	branches					582:589	the branches	578:589	the branches of HFSGF	578:598	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	0	4	from	analysis	11:18	arg1	activity					125:132	its anti-lung cancer activity	104:132	its anti-lung cancer activity	104:132	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	0	4	from	analysis	11:18	arg1	fusiforme					90:98	Sargassum fusiforme	80:98	Sargassum fusiforme	80:98	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	4	5	theme	1,4-linked	842:851	arg1	GlcA					874:877	GlcA	874:877	GlcA	874:877	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	5	theme	1,4-linked	842:851	arg1	acid					868:871	1,4-linked β-D-glucuronic acid	842:871	1,4-linked β-D-glucuronic acid (GlcA)	842:878	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	1	6	theme	Sargassum	176:184	arg1	fusiforme					186:194	Sargassum fusiforme	176:194	Sargassum fusiforme	176:194	Polysaccharide (HFSGF) was purified from Sargassum fusiforme.					
32004605	4	7	from	ends	560:563	arg1	HFSGF-S					475:481	HFSGF-S	475:481	HFSGF-S	475:481	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	7	from	ends	560:563	arg1	mixture					489:495	a mixture	487:495	a mixture of sulfated galacto-fuco-oligomers	487:530	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	7	from	ends	560:563	arg1	galactofucan					616:627	a sulfated galactofucan	605:627	a sulfated galactofucan	605:627	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	0	8	theme	cancer	118:123	arg1	activity					125:132	its anti-lung cancer activity	104:132	its anti-lung cancer activity	104:132	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	4	9	theme	HFSGF	774:778	arg1	backbone					762:769	the backbone	758:769	the backbone	758:769	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	6	10	theme	HFSGF-H	1140:1146	arg1	activities					1114:1123	The anti-lung cancer activities	1093:1123	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo	1093:1232	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	4	11	theme	sulfated	607:614	arg1	galactofucan					616:627	a sulfated galactofucan	605:627	a sulfated galactofucan	605:627	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	0	12	theme	anti-lung	108:116	arg1	activity					125:132	its anti-lung cancer activity	104:132	its anti-lung cancer activity	104:132	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	4	13	theme	galacto-fuco-oligomers	509:530	arg1	HFSGF-S					475:481	HFSGF-S	475:481	HFSGF-S	475:481	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	13	theme	galacto-fuco-oligomers	509:530	arg1	mixture					489:495	a mixture	487:495	a mixture of sulfated galacto-fuco-oligomers	487:530	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	13	theme	galacto-fuco-oligomers	509:530	arg1	galactofucan					616:627	a sulfated galactofucan	605:627	a sulfated galactofucan	605:627	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	6	14	theme	lung	1162:1165	arg1	cells					1179:1183	human lung cancer A549 cells	1156:1183	human lung cancer A549 cells	1156:1183	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	6	15	theme	growth	1219:1224	arg1	activities					1114:1123	The anti-lung cancer activities	1093:1123	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo	1093:1232	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	8	16	theme	lung	1471:1474	arg1	cancer					1476:1481	lung cancer	1471:1481	lung cancer	1471:1481	than HFSGF-L, indicating that HFSGF-H might be a leading compound for a potential new therapeutics for the treatment of lung cancer.					
32004605	4	17	theme	1,3-linked	936:945	arg1	galactofucan					904:915	sulfated galactofucan	895:915	sulfated galactofucan	895:915	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	17	theme	1,3-linked	936:945	arg1	α-L-fucan					947:955	1,3-linked α-L-fucan	936:955	1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal	936:1015	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	18	theme	sulfated	500:507	arg1	galacto-fuco-oligomers					509:530	sulfated galacto-fuco-oligomers	500:530	sulfated galacto-fuco-oligomers	500:530	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	6	19	theme	tumor	1213:1217	arg1	growth					1219:1224	A549 xenograft tumor growth	1198:1224	A549 xenograft tumor growth	1198:1224	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	6	20	theme	HFSGF-L	1128:1134	arg1	activities					1114:1123	The anti-lung cancer activities	1093:1123	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo	1093:1232	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	4	21	dep	composed	785:792	arg1	branched					881:888	branched	881:888	branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal	881:1015	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	3	22	theme	nuclear	378:384	arg1	spectroscopy					405:416	nuclear magnetic resonance spectroscopy	378:416	nuclear magnetic resonance spectroscopy	378:416	Compositional analysis, mass spectrometry and nuclear magnetic resonance spectroscopy were used to elucidate the structural features of HFSGF.					
32004605	0	23	theme	sulfated	31:38	arg1	galacto-fuco-xylo-glucurono-mannan					40:73	a novel sulfated galacto-fuco-xylo-glucurono-mannan	23:73	a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity	23:132	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	7	24	theme	%	1347:1347	arg1	inhibition					1333:1342	tumor inhibition	1327:1342	tumor inhibition ~51%.	1327:1348	HFSGF-H had higher activity in vitro (IC50 ~12 mg/mL for 24 h) and in vivo (tumor inhibition ~51%.)					
32004605	4	25	link	1,2-linked	809:818	arg1	Man					833:835	Man	833:835	Man	833:835	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	25	link	1,2-linked	809:818	arg1	α-D-mannose					820:830	1,2-linked α-D-mannose	809:830	1,2-linked α-D-mannose (Man)	809:836	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	8	26	theme	new	1433:1435	arg1	therapeutics					1437:1448	a potential new therapeutics	1421:1448	a potential new therapeutics for the treatment of lung cancer	1421:1481	than HFSGF-L, indicating that HFSGF-H might be a leading compound for a potential new therapeutics for the treatment of lung cancer.					
32004605	6	27	theme	A549	1198:1201	arg1	growth					1219:1224	A549 xenograft tumor growth	1198:1224	A549 xenograft tumor growth	1198:1224	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	7	28	theme	tumor	1327:1331	arg1	inhibition					1333:1342	tumor inhibition	1327:1342	tumor inhibition ~51%.	1327:1348	HFSGF-H had higher activity in vitro (IC50 ~12 mg/mL for 24 h) and in vivo (tumor inhibition ~51%.)					
32004605	7	29	theme	higher	1263:1268	arg1	activity					1270:1277	higher activity	1263:1277	higher activity	1263:1277	HFSGF-H had higher activity in vitro (IC50 ~12 mg/mL for 24 h) and in vivo (tumor inhibition ~51%.)					
32004605	0	30	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.	0:133	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	7	31	dep	vitro	1282:1286	arg1	~12 mg/mL					1294:1302	IC50 ~12 mg/mL	1289:1302	IC50 ~12 mg/mL for 24 h	1289:1311	HFSGF-H had higher activity in vitro (IC50 ~12 mg/mL for 24 h) and in vivo (tumor inhibition ~51%.)					
32004605	4	32	theme	terminal	551:558	arg1	ends					560:563	the branches terminal ends	538:563	the branches terminal ends	538:563	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	6	33	theme	cancer	1107:1112	arg1	activities					1114:1123	The anti-lung cancer activities	1093:1123	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo	1093:1232	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	2	34	dep	components	289:298	arg1	HFSGF-L					310:316	HFSGF-L	310:316	HFSGF-L	310:316	Autohydrolysis and gel column chromatography were performed to fractionate HFSGF into three components (HFSGF-S, HFSGF-L and HFSGF-H).					
32004605	2	34	dep	components	289:298	arg1	HFSGF-S					301:307	HFSGF-S	301:307	HFSGF-S	301:307	Autohydrolysis and gel column chromatography were performed to fractionate HFSGF into three components (HFSGF-S, HFSGF-L and HFSGF-H).					
32004605	2	34	dep	components	289:298	arg1	components					289:298	three components	283:298	three components (HFSGF-S, HFSGF-L and HFSGF-H)	283:329	Autohydrolysis and gel column chromatography were performed to fractionate HFSGF into three components (HFSGF-S, HFSGF-L and HFSGF-H).					
32004605	2	34	dep	components	289:298	arg1	HFSGF-H					322:328	HFSGF-H	322:328	HFSGF-H	322:328	Autohydrolysis and gel column chromatography were performed to fractionate HFSGF into three components (HFSGF-S, HFSGF-L and HFSGF-H).					
32004605	4	35	theme	sulfated	895:902	arg1	galactofucan					904:915	sulfated galactofucan	895:915	sulfated galactofucan	895:915	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	35	theme	sulfated	895:902	arg1	α-L-fucan					947:955	1,3-linked α-L-fucan	936:955	1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal	936:1015	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	6	36	theme	A549	1174:1177	arg1	cells					1179:1183	human lung cancer A549 cells	1156:1183	human lung cancer A549 cells	1156:1183	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	6	37	theme	anti-lung	1097:1105	arg1	activities					1114:1123	The anti-lung cancer activities	1093:1123	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo	1093:1232	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	8	38	theme	leading	1400:1406	arg1	compound					1408:1415	a leading compound	1398:1415	a leading compound for a potential new therapeutics for the treatment of lung cancer	1398:1481	than HFSGF-L, indicating that HFSGF-H might be a leading compound for a potential new therapeutics for the treatment of lung cancer.					
32004605	8	38	theme	leading	1400:1406	arg1	HFSGF-H					1381:1387	HFSGF-H	1381:1387	HFSGF-H	1381:1387	than HFSGF-L, indicating that HFSGF-H might be a leading compound for a potential new therapeutics for the treatment of lung cancer.					
32004605	4	39	link	1,4-linked	842:851	arg1	GlcA					874:877	GlcA	874:877	GlcA	874:877	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	39	link	1,4-linked	842:851	arg1	acid					868:871	1,4-linked β-D-glucuronic acid	842:871	1,4-linked β-D-glucuronic acid (GlcA)	842:878	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	40	theme	HFSGF	594:598	arg1	HFSGF-L					569:575	HFSGF-L	569:575	HFSGF-L	569:575	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	40	theme	HFSGF	594:598	arg1	branches					582:589	the branches	578:589	the branches of HFSGF	578:598	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	41	from	mixture	489:495	arg1	HFSGF-L					569:575	HFSGF-L	569:575	HFSGF-L	569:575	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	41	from	mixture	489:495	arg1	branches					582:589	the branches	578:589	the branches of HFSGF	578:598	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	3	42	theme	structural	445:454	arg1	features					456:463	the structural features	441:463	the structural features of HFSGF	441:472	Compositional analysis, mass spectrometry and nuclear magnetic resonance spectroscopy were used to elucidate the structural features of HFSGF.					
32004605	4	43	theme	sulfated	920:927	arg1	fucan					929:933	sulfated fucan	920:933	sulfated fucan	920:933	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	44	dep	α-L-fucan	666:674	arg1	interspersed					707:718	interspersed	707:718	interspersed with galactose (Gal)	707:739	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	44	dep	α-L-fucan	666:674	arg1	sulfated					676:683	sulfated	676:683	sulfated at C2/4 and/or C4	676:701	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	3	45	theme	HFSGF	468:472	arg1	features					456:463	the structural features	441:463	the structural features of HFSGF	441:472	Compositional analysis, mass spectrometry and nuclear magnetic resonance spectroscopy were used to elucidate the structural features of HFSGF.					
32004605	4	46	theme	α-L-fucan	666:674	arg1	backbone					643:650	a backbone	641:650	a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal)	641:739	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	0	47	theme	novel	25:29	arg1	galacto-fuco-xylo-glucurono-mannan					40:73	a novel sulfated galacto-fuco-xylo-glucurono-mannan	23:73	a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity	23:132	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	8	48	theme	cancer	1476:1481	arg1	treatment					1458:1466	the treatment	1454:1466	the treatment of lung cancer	1454:1481	than HFSGF-L, indicating that HFSGF-H might be a leading compound for a potential new therapeutics for the treatment of lung cancer.					
32004605	3	49	used	used	423:426	arg2	analysis					346:353	Compositional analysis	332:353	Compositional analysis	332:353	Compositional analysis, mass spectrometry and nuclear magnetic resonance spectroscopy were used to elucidate the structural features of HFSGF.					
32004605	3	49	used	used	423:426	arg2	spectrometry					361:372	mass spectrometry	356:372	mass spectrometry	356:372	Compositional analysis, mass spectrometry and nuclear magnetic resonance spectroscopy were used to elucidate the structural features of HFSGF.					
32004605	3	49	used	used	423:426	arg2	spectroscopy					405:416	nuclear magnetic resonance spectroscopy	378:416	nuclear magnetic resonance spectroscopy	378:416	Compositional analysis, mass spectrometry and nuclear magnetic resonance spectroscopy were used to elucidate the structural features of HFSGF.					
32004605	0	50	from	fusiforme	90:98	arg1	galacto-fuco-xylo-glucurono-mannan					40:73	a novel sulfated galacto-fuco-xylo-glucurono-mannan	23:73	a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity	23:132	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	0	50	from	fusiforme	90:98	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.	0:133	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	4	51	theme	1,3-linked	655:664	arg1	α-L-fucan					666:674	1,3-linked α-L-fucan	655:674	1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal)	655:739	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	3	52	theme	magnetic	386:393	arg1	spectroscopy					405:416	nuclear magnetic resonance spectroscopy	378:416	nuclear magnetic resonance spectroscopy	378:416	Compositional analysis, mass spectrometry and nuclear magnetic resonance spectroscopy were used to elucidate the structural features of HFSGF.					
32004605	4	53	link	1,3-linked	936:945	arg1	galactofucan					904:915	sulfated galactofucan	895:915	sulfated galactofucan	895:915	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	53	link	1,3-linked	936:945	arg1	α-L-fucan					947:955	1,3-linked α-L-fucan	936:955	1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal	936:1015	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	5	54	theme	fucose	1023:1028	arg1	residues					1036:1043	Some fucose (Fuc) residues	1018:1043	Some fucose (Fuc) residues	1018:1043	Some fucose (Fuc) residues were also partially branched with xylose (Xyl).					
32004605	0	55	theme	galacto-fuco-xylo-glucurono-mannan	40:73	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.	0:133	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	3	56	theme	Compositional	332:344	arg1	analysis					346:353	Compositional analysis	332:353	Compositional analysis	332:353	Compositional analysis, mass spectrometry and nuclear magnetic resonance spectroscopy were used to elucidate the structural features of HFSGF.					
32004605	4	57	theme	1,2-linked	809:818	arg1	Man					833:835	Man	833:835	Man	833:835	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	57	theme	1,2-linked	809:818	arg1	α-D-mannose					820:830	1,2-linked α-D-mannose	809:830	1,2-linked α-D-mannose (Man)	809:836	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	7	58	contain	had	1259:1261	arg1	HFSGF-H					1251:1257	HFSGF-H	1251:1257	HFSGF-H	1251:1257	HFSGF-H had higher activity in vitro (IC50 ~12 mg/mL for 24 h) and in vivo (tumor inhibition ~51%.)					
32004605	7	58	contain	had	1259:1261	arg2	activity					1270:1277	higher activity	1263:1277	higher activity	1263:1277	HFSGF-H had higher activity in vitro (IC50 ~12 mg/mL for 24 h) and in vivo (tumor inhibition ~51%.)					
32004605	0	59	from	activity	125:132	arg1	galacto-fuco-xylo-glucurono-mannan					40:73	a novel sulfated galacto-fuco-xylo-glucurono-mannan	23:73	a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity	23:132	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	0	59	from	activity	125:132	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.	0:133	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	4	60	contain	containing	630:639	arg2	backbone					643:650	a backbone	641:650	a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal)	641:739	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	60	contain	containing	630:639	arg1	galactofucan					616:627	a sulfated galactofucan	605:627	a sulfated galactofucan	605:627	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	7	61	theme	~51	1344:1346	arg1	%					1347:1347	%	1347:1347	%	1347:1347	HFSGF-H had higher activity in vitro (IC50 ~12 mg/mL for 24 h) and in vivo (tumor inhibition ~51%.)					
32004605	4	62	dep	α-L-fucan	947:955	arg1	sulfated					957:964	sulfated	957:964	sulfated at C2/4 and/or C4	957:982	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	4	62	dep	α-L-fucan	947:955	arg1	interspersed					995:1006	interspersed	995:1006	partly interspersed with Gal	988:1015	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	2	63	theme	column	220:225	arg1	chromatography					227:240	gel column chromatography	216:240	gel column chromatography	216:240	Autohydrolysis and gel column chromatography were performed to fractionate HFSGF into three components (HFSGF-S, HFSGF-L and HFSGF-H).					
32004605	4	64	link	1,3-linked	655:664	arg1	α-L-fucan					666:674	1,3-linked α-L-fucan	655:674	1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal)	655:739	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	3	65	theme	mass	356:359	arg1	spectrometry					361:372	mass spectrometry	356:372	mass spectrometry	356:372	Compositional analysis, mass spectrometry and nuclear magnetic resonance spectroscopy were used to elucidate the structural features of HFSGF.					
32004605	2	66	theme	gel	216:218	arg1	chromatography					227:240	gel column chromatography	216:240	gel column chromatography	216:240	Autohydrolysis and gel column chromatography were performed to fractionate HFSGF into three components (HFSGF-S, HFSGF-L and HFSGF-H).					
32004605	6	67	theme	human	1156:1160	arg1	cells					1179:1183	human lung cancer A549 cells	1156:1183	human lung cancer A549 cells	1156:1183	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	7	68	dep	vivo	1321:1324	arg1	inhibition					1333:1342	tumor inhibition	1327:1342	tumor inhibition ~51%.	1327:1348	HFSGF-H had higher activity in vitro (IC50 ~12 mg/mL for 24 h) and in vivo (tumor inhibition ~51%.)					
32004605	0	69	theme	Sargassum	80:88	arg1	fusiforme					90:98	Sargassum fusiforme	80:98	Sargassum fusiforme	80:98	Structural analysis of a novel sulfated galacto-fuco-xylo-glucurono-mannan from Sargassum fusiforme and its anti-lung cancer activity.					
32004605	4	70	theme	branches	542:549	arg1	ends					560:563	the branches terminal ends	538:563	the branches terminal ends	538:563	HFSGF-S was a mixture of sulfated galacto-fuco-oligomers, from the branches terminal ends; in HFSGF-L, the branches of HFSGF, was a sulfated galactofucan, containing a backbone of 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and interspersed with galactose (Gal); and in HFSGF-H, the backbone of HFSGF, was composed of alternating 1,2-linked α-D-mannose (Man) and 1,4-linked β-D-glucuronic acid (GlcA), branched with sulfated galactofucan or sulfated fucan, 1,3-linked α-L-fucan sulfated at C2/4 and/or C4 and partly interspersed with Gal.					
32004605	7	71	theme	IC50	1289:1292	arg1	~12 mg/mL					1294:1302	IC50 ~12 mg/mL	1289:1302	IC50 ~12 mg/mL for 24 h	1289:1311	HFSGF-H had higher activity in vitro (IC50 ~12 mg/mL for 24 h) and in vivo (tumor inhibition ~51%.)					
32004605	6	72	theme	cancer	1167:1172	arg1	cells					1179:1183	human lung cancer A549 cells	1156:1183	human lung cancer A549 cells	1156:1183	The anti-lung cancer activities of HFSGF-L and HFSGF-H against human lung cancer A549 cells in vitro and A549 xenograft tumor growth in vivo were determined.					
32004605	3	73	theme	resonance	395:403	arg1	spectroscopy					405:416	nuclear magnetic resonance spectroscopy	378:416	nuclear magnetic resonance spectroscopy	378:416	Compositional analysis, mass spectrometry and nuclear magnetic resonance spectroscopy were used to elucidate the structural features of HFSGF.					
34202227	0	0	theme	Gut	82:84	arg1	Microbiome					86:95	a Synthetic Human Gut Microbiome	64:95	a Synthetic Human Gut Microbiome	64:95	Concentrated Raw Fibers Enhance the Fiber-Degrading Capacity of a Synthetic Human Gut Microbiome.					
34202227	4	1	dep	employed	537:544	arg1	characterized					640:652	characterized	640:652	characterized a priori for their ability to metabolize a collection of fibers in vitro	640:725	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	6	2	from	oat	980:982	arg1	fibers					963:968	-containing fibers	951:968	dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	913:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	1	3	theme	human	150:154	arg1	microbiome					160:169	the human gut microbiome	146:169	the human gut microbiome	146:169	The consumption of prebiotic fibers to modulate the human gut microbiome is a promising strategy to positively impact health.					
34202227	4	4	theme	14-member	585:593	arg1	microbiome					615:624	a 14-member synthetic human gut microbiome	583:624	a 14-member synthetic human gut microbiome (SM)	583:629	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	4	4	theme	14-member	585:593	arg1	SM					627:628	SM	627:628	SM	627:628	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	0	5	theme	Human	76:80	arg1	Microbiome					86:95	a Synthetic Human Gut Microbiome	64:95	a Synthetic Human Gut Microbiome	64:95	Concentrated Raw Fibers Enhance the Fiber-Degrading Capacity of a Synthetic Human Gut Microbiome.					
34202227	5	6	contain	contains	736:743	arg2	strains					758:764	14 different strains	745:764	14 different strains belonging to five distinct phyla	745:797	This SM contains 14 different strains belonging to five distinct phyla.					
34202227	5	6	contain	contains	736:743	arg1	SM					733:734	This SM	728:734	This SM	728:734	This SM contains 14 different strains belonging to five distinct phyla.					
34202227	2	7	from	recommendations	339:353	arg1	amount					372:377	amount	372:377	amount	372:377	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	2	7	from	recommendations	339:353	arg1	supplements					388:398	fiber supplements	382:398	fiber supplements	382:398	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	2	7	from	recommendations	339:353	arg1	source					362:367	source	362:367	source	362:367	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	1	8	theme	gut	156:158	arg1	microbiome					160:169	the human gut microbiome	146:169	the human gut microbiome	146:169	The consumption of prebiotic fibers to modulate the human gut microbiome is a promising strategy to positively impact health.					
34202227	6	9	theme	-containing	951:961	arg1	fibers					963:968	-containing fibers	951:968	dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	913:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	8	10	theme	fiber-degrading	1402:1416	arg1	activity					1439:1446	bacterial fiber-degrading α-glucosidase enzyme activity	1392:1446	bacterial fiber-degrading α-glucosidase enzyme activity	1392:1446	These results were corroborated by a general increase of bacterial fiber-degrading α-glucosidase enzyme activity.					
34202227	6	11	from	psyllium	985:992	arg1	fibers					963:968	-containing fibers	951:968	dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	913:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	4	12	theme	human	605:609	arg1	microbiome					615:624	a 14-member synthetic human gut microbiome	583:624	a 14-member synthetic human gut microbiome (SM)	583:629	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	4	12	theme	human	605:609	arg1	SM					627:628	SM	627:628	SM	627:628	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	0	13	theme	Microbiome	86:95	arg1	Capacity					52:59	the Fiber-Degrading Capacity	32:59	the Fiber-Degrading Capacity of a Synthetic Human Gut Microbiome	32:95	Concentrated Raw Fibers Enhance the Fiber-Degrading Capacity of a Synthetic Human Gut Microbiome.					
34202227	2	14	theme	microbiome	280:289	arg1	variances					316:324	its inter-individual variances	295:324	its inter-individual variances	295:324	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	2	14	theme	microbiome	280:289	arg1	complexity					262:271	the compositional complexity	244:271	the compositional complexity of the microbiome	244:289	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	2	15	dep	source	362:367	arg1	the					358:360	the	358:360	the	358:360	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	3	16	theme	in	491:492	arg1	models					499:504	tractable in vitro and in vivo models	468:504	tractable in vitro and in vivo models	468:504	This problem is further compounded by availability of tractable in vitro and in vivo models to validate certain fibers.					
34202227	6	17	theme	compositional	1018:1030	arg1	alterations					1047:1057	the compositional and functional alterations	1014:1057	the compositional and functional alterations in the SM	1014:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	6	18	theme	functional	1036:1045	arg1	alterations					1047:1057	the compositional and functional alterations	1014:1057	the compositional and functional alterations in the SM	1014:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	4	19	theme	synthetic	595:603	arg1	microbiome					615:624	a 14-member synthetic human gut microbiome	583:624	a 14-member synthetic human gut microbiome (SM)	583:629	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	4	19	theme	synthetic	595:603	arg1	SM					627:628	SM	627:628	SM	627:628	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	6	20	theme	studies	860:866	arg1	fibers					823:828	soluble purified fibers	806:828	soluble purified fibers	806:828	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	6	20	theme	studies	860:866	arg1	subject					849:855	a common subject	840:855	a common subject of studies	840:866	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	6	21	from	wheat	995:999	arg1	fibers					963:968	-containing fibers	951:968	dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	913:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	7	22	theme	CRF	1122:1124	arg1	supplementation					1126:1140	CRF supplementation	1122:1140	CRF supplementation	1122:1140	We demonstrate that, compared to a fiber-free diet, CRF supplementation increased the abundance of fiber-degraders, namely Eubacterium rectale, Roseburia intestinalis and Bacteroides ovatus and decreased the abundance of the mucin-degrader Akkermansia muciniphila.					
34202227	7	23	theme	Akkermansia	1310:1320	arg1	muciniphila					1322:1332	the mucin-degrader Akkermansia muciniphila	1291:1332	the mucin-degrader Akkermansia muciniphila	1291:1332	We demonstrate that, compared to a fiber-free diet, CRF supplementation increased the abundance of fiber-degraders, namely Eubacterium rectale, Roseburia intestinalis and Bacteroides ovatus and decreased the abundance of the mucin-degrader Akkermansia muciniphila.					
34202227	0	24	theme	Raw	13:15	arg1	Fibers					17:22	Concentrated Raw Fibers	0:22	Concentrated Raw Fibers	0:22	Concentrated Raw Fibers Enhance the Fiber-Degrading Capacity of a Synthetic Human Gut Microbiome.					
34202227	5	25	theme	different	748:756	arg1	strains					758:764	14 different strains	745:764	14 different strains belonging to five distinct phyla	745:797	This SM contains 14 different strains belonging to five distinct phyla.					
34202227	6	26	from	pea	975:977	arg1	fibers					963:968	-containing fibers	951:968	dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	913:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	2	27	theme	supplements	388:398	arg1	amount					372:377	amount	372:377	amount	372:377	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	2	27	theme	supplements	388:398	arg1	supplements					388:398	fiber supplements	382:398	fiber supplements	382:398	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	2	27	theme	supplements	388:398	arg1	source					362:367	source	362:367	source	362:367	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	0	28	theme	Concentrated	0:11	arg1	Fibers					17:22	Concentrated Raw Fibers	0:22	Concentrated Raw Fibers	0:22	Concentrated Raw Fibers Enhance the Fiber-Degrading Capacity of a Synthetic Human Gut Microbiome.					
34202227	4	29	theme	mouse	560:564	arg1	model					566:570	a gnotobiotic mouse model	546:570	a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM)	546:629	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	6	30	from	apple-on	1005:1012	arg1	fibers					963:968	-containing fibers	951:968	dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	913:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	4	31	contain	containing	572:581	arg2	SM					627:628	SM	627:628	SM	627:628	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	4	31	contain	containing	572:581	arg2	microbiome					615:624	a 14-member synthetic human gut microbiome	583:624	a 14-member synthetic human gut microbiome (SM)	583:629	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	4	31	contain	containing	572:581	arg1	model					566:570	a gnotobiotic mouse model	546:570	a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM)	546:629	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	2	32	theme	fiber	382:386	arg1	supplements					388:398	fiber supplements	382:398	fiber supplements	382:398	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	7	33	theme	fiber-free	1105:1114	arg1	diet					1116:1119	a fiber-free diet	1103:1119	a fiber-free diet	1103:1119	We demonstrate that, compared to a fiber-free diet, CRF supplementation increased the abundance of fiber-degraders, namely Eubacterium rectale, Roseburia intestinalis and Bacteroides ovatus and decreased the abundance of the mucin-degrader Akkermansia muciniphila.					
34202227	4	34	theme	gnotobiotic	548:558	arg1	model					566:570	a gnotobiotic mouse model	546:570	a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM)	546:629	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	2	35	theme	inter-individual	299:314	arg1	variances					316:324	its inter-individual variances	295:324	its inter-individual variances	295:324	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	3	36	theme	in	478:479	arg1	models					499:504	tractable in vitro and in vivo models	468:504	tractable in vitro and in vivo models	468:504	This problem is further compounded by availability of tractable in vitro and in vivo models to validate certain fibers.					
34202227	6	37	dep	pea	975:977	arg1	alterations					1047:1057	the compositional and functional alterations	1014:1057	the compositional and functional alterations in the SM	1014:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	8	38	theme	activity	1439:1446	arg1	increase					1380:1387	a general increase	1370:1387	a general increase of bacterial fiber-degrading α-glucosidase enzyme activity	1370:1446	These results were corroborated by a general increase of bacterial fiber-degrading α-glucosidase enzyme activity.					
34202227	0	39	theme	Fiber-Degrading	36:50	arg1	Capacity					52:59	the Fiber-Degrading Capacity	32:59	the Fiber-Degrading Capacity of a Synthetic Human Gut Microbiome	32:95	Concentrated Raw Fibers Enhance the Fiber-Degrading Capacity of a Synthetic Human Gut Microbiome.					
34202227	3	40	theme	models	499:504	arg1	availability					452:463	availability	452:463	availability of tractable in vitro and in vivo models to validate certain fibers	452:531	This problem is further compounded by availability of tractable in vitro and in vivo models to validate certain fibers.					
34202227	9	41	theme	CRFs	1495:1498	arg1	ability					1484:1490	the ability	1480:1490	the ability of CRFs to enhance the microbial fiber-degrading capacity	1480:1548	Overall, our results highlight the ability of CRFs to enhance the microbial fiber-degrading capacity.					
34202227	6	42	theme	common	842:847	arg1	fibers					823:828	soluble purified fibers	806:828	soluble purified fibers	806:828	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	6	42	theme	common	842:847	arg1	subject					849:855	a common subject	840:855	a common subject of studies	840:866	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	6	43	theme	fibers	938:943	arg1	effects					902:908	the effects	898:908	the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	898:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	1	44	theme	promising	176:184	arg1	strategy					186:193	a promising strategy	174:193	a promising strategy to positively impact health	174:221	The consumption of prebiotic fibers to modulate the human gut microbiome is a promising strategy to positively impact health.					
34202227	1	44	theme	promising	176:184	arg1	consumption					102:112	The consumption	98:112	The consumption of prebiotic fibers to modulate the human gut microbiome	98:169	The consumption of prebiotic fibers to modulate the human gut microbiome is a promising strategy to positively impact health.					
34202227	9	45	theme	fiber-degrading	1525:1539	arg1	capacity					1541:1548	the microbial fiber-degrading capacity	1511:1548	the microbial fiber-degrading capacity	1511:1548	Overall, our results highlight the ability of CRFs to enhance the microbial fiber-degrading capacity.					
34202227	7	46	theme	mucin-degrader	1295:1308	arg1	muciniphila					1322:1332	the mucin-degrader Akkermansia muciniphila	1291:1332	the mucin-degrader Akkermansia muciniphila	1291:1332	We demonstrate that, compared to a fiber-free diet, CRF supplementation increased the abundance of fiber-degraders, namely Eubacterium rectale, Roseburia intestinalis and Bacteroides ovatus and decreased the abundance of the mucin-degrader Akkermansia muciniphila.					
34202227	6	47	theme	raw	934:936	arg1	CRFs					946:949	CRFs	946:949	CRFs	946:949	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	6	47	theme	raw	934:936	arg1	fibers					938:943	dietary concentrated raw fibers	913:943	dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	913:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	8	48	theme	general	1372:1378	arg1	increase					1380:1387	a general increase	1370:1387	a general increase of bacterial fiber-degrading α-glucosidase enzyme activity	1370:1446	These results were corroborated by a general increase of bacterial fiber-degrading α-glucosidase enzyme activity.					
34202227	3	49	theme	tractable	468:476	arg1	models					499:504	tractable in vitro and in vivo models	468:504	tractable in vitro and in vivo models	468:504	This problem is further compounded by availability of tractable in vitro and in vivo models to validate certain fibers.					
34202227	6	50	theme	concentrated	921:932	arg1	CRFs					946:949	CRFs	946:949	CRFs	946:949	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	6	50	theme	concentrated	921:932	arg1	fibers					938:943	dietary concentrated raw fibers	913:943	dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	913:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	7	51	theme	muciniphila	1322:1332	arg1	abundance					1278:1286	the abundance	1274:1286	the abundance of the mucin-degrader Akkermansia muciniphila	1274:1332	We demonstrate that, compared to a fiber-free diet, CRF supplementation increased the abundance of fiber-degraders, namely Eubacterium rectale, Roseburia intestinalis and Bacteroides ovatus and decreased the abundance of the mucin-degrader Akkermansia muciniphila.					
34202227	8	52	theme	bacterial	1392:1400	arg1	activity					1439:1446	bacterial fiber-degrading α-glucosidase enzyme activity	1392:1446	bacterial fiber-degrading α-glucosidase enzyme activity	1392:1446	These results were corroborated by a general increase of bacterial fiber-degrading α-glucosidase enzyme activity.					
34202227	6	53	theme	dietary	913:919	arg1	CRFs					946:949	CRFs	946:949	CRFs	946:949	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	6	53	theme	dietary	913:919	arg1	fibers					938:943	dietary concentrated raw fibers	913:943	dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	913:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	1	54	theme	prebiotic	117:125	arg1	fibers					127:132	prebiotic fibers	117:132	prebiotic fibers	117:132	The consumption of prebiotic fibers to modulate the human gut microbiome is a promising strategy to positively impact health.					
34202227	4	55	theme	fibers	711:716	arg1	collection					697:706	a collection	695:706	a collection of fibers	695:716	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	6	56	dep	fibers	938:943	arg1	fibers					963:968	-containing fibers	951:968	dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM	913:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	8	57	theme	α-glucosidase	1418:1430	arg1	activity					1439:1446	bacterial fiber-degrading α-glucosidase enzyme activity	1392:1446	bacterial fiber-degrading α-glucosidase enzyme activity	1392:1446	These results were corroborated by a general increase of bacterial fiber-degrading α-glucosidase enzyme activity.					
34202227	9	58	theme	microbial	1515:1523	arg1	capacity					1541:1548	the microbial fiber-degrading capacity	1511:1548	the microbial fiber-degrading capacity	1511:1548	Overall, our results highlight the ability of CRFs to enhance the microbial fiber-degrading capacity.					
34202227	1	59	theme	fibers	127:132	arg1	strategy					186:193	a promising strategy	174:193	a promising strategy to positively impact health	174:221	The consumption of prebiotic fibers to modulate the human gut microbiome is a promising strategy to positively impact health.					
34202227	1	59	theme	fibers	127:132	arg1	consumption					102:112	The consumption	98:112	The consumption of prebiotic fibers to modulate the human gut microbiome	98:169	The consumption of prebiotic fibers to modulate the human gut microbiome is a promising strategy to positively impact health.					
34202227	6	60	theme	purified	814:821	arg1	fibers					823:828	soluble purified fibers	806:828	soluble purified fibers	806:828	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	6	60	theme	purified	814:821	arg1	subject					849:855	a common subject	840:855	a common subject of studies	840:866	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	2	61	theme	compositional	248:260	arg1	complexity					262:271	the compositional complexity	244:271	the compositional complexity of the microbiome	244:289	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	2	62	theme	generalized	327:337	arg1	recommendations					339:353	generalized recommendations	327:353	generalized recommendations on the source or amount of fiber supplements	327:398	Nevertheless, given the compositional complexity of the microbiome and its inter-individual variances, generalized recommendations on the source or amount of fiber supplements remain vague.					
34202227	4	63	theme	gut	611:613	arg1	microbiome					615:624	a 14-member synthetic human gut microbiome	583:624	a 14-member synthetic human gut microbiome (SM)	583:629	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	4	63	theme	gut	611:613	arg1	SM					627:628	SM	627:628	SM	627:628	We employed a gnotobiotic mouse model containing a 14-member synthetic human gut microbiome (SM) in vivo, characterized a priori for their ability to metabolize a collection of fibers in vitro.					
34202227	0	64	theme	Synthetic	66:74	arg1	Microbiome					86:95	a Synthetic Human Gut Microbiome	64:95	a Synthetic Human Gut Microbiome	64:95	Concentrated Raw Fibers Enhance the Fiber-Degrading Capacity of a Synthetic Human Gut Microbiome.					
34202227	6	65	theme	soluble	806:812	arg1	fibers					823:828	soluble purified fibers	806:828	soluble purified fibers	806:828	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	6	65	theme	soluble	806:812	arg1	subject					849:855	a common subject	840:855	a common subject of studies	840:866	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	3	66	dep	in	478:479	arg1	vitro					481:485	vitro	481:485	vitro	481:485	This problem is further compounded by availability of tractable in vitro and in vivo models to validate certain fibers.					
34202227	6	67	from	alterations	1047:1057	arg1	SM					1066:1067	the SM	1062:1067	the SM	1062:1067	Since soluble purified fibers have been a common subject of studies, we specifically investigated the effects of dietary concentrated raw fibers (CRFs)-containing fibers from pea, oat, psyllium, wheat and apple-on the compositional and functional alterations in the SM.					
34202227	3	68	theme	certain	518:524	arg1	fibers					526:531	certain fibers	518:531	certain fibers	518:531	This problem is further compounded by availability of tractable in vitro and in vivo models to validate certain fibers.					
34202227	3	69	dep	in	491:492	arg1	vivo					494:497	vivo	494:497	vivo	494:497	This problem is further compounded by availability of tractable in vitro and in vivo models to validate certain fibers.					
34202227	7	70	theme	fiber-degraders	1169:1183	arg1	abundance					1156:1164	the abundance	1152:1164	the abundance of fiber-degraders, namely Eubacterium rectale, Roseburia intestinalis and Bacteroides ovatus	1152:1258	We demonstrate that, compared to a fiber-free diet, CRF supplementation increased the abundance of fiber-degraders, namely Eubacterium rectale, Roseburia intestinalis and Bacteroides ovatus and decreased the abundance of the mucin-degrader Akkermansia muciniphila.					
34202227	8	71	theme	enzyme	1432:1437	arg1	activity					1439:1446	bacterial fiber-degrading α-glucosidase enzyme activity	1392:1446	bacterial fiber-degrading α-glucosidase enzyme activity	1392:1446	These results were corroborated by a general increase of bacterial fiber-degrading α-glucosidase enzyme activity.					
34202227	5	72	theme	distinct	784:791	arg1	phyla					793:797	five distinct phyla	779:797	five distinct phyla	779:797	This SM contains 14 different strains belonging to five distinct phyla.					
32651818	2	0	dep	isotypes	472:479	arg1	isotypes					472:479	its isotypes	468:479	its isotypes IgG1 and, especially, IgG2a	468:507	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	0	dep	isotypes	472:479	arg1	IgG2a					503:507	IgG2a	503:507	IgG2a	503:507	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	0	dep	isotypes	472:479	arg1	IgG1					481:484	IgG1	481:484	IgG1	481:484	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	1	1	theme	vaccine	155:161	arg1	compositions					163:174	experimental vaccine compositions	142:174	experimental vaccine compositions with ovalbumin	142:189	Adjuvant activity of fucoidans in experimental vaccine compositions with ovalbumin was studied on a mouse model.					
32651818	2	2	from	fucoidan	305:312	arg1	combination					317:327	combination	317:327	combination with polyphenols	317:344	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	3	3	theme	adjuvant	696:703	arg1	aluminum					677:684	the traditional licensed aluminum hydroxide adjuvant	652:703	the traditional licensed aluminum hydroxide adjuvant	652:703	The adjuvant effect of native and structurally modified fucoidans is slightly inferior to that of the traditional licensed aluminum hydroxide adjuvant.					
32651818	1	4	with	compositions	163:174	arg1	ovalbumin					181:189	ovalbumin	181:189	ovalbumin	181:189	Adjuvant activity of fucoidans in experimental vaccine compositions with ovalbumin was studied on a mouse model.					
32651818	4	5	from	adjuvants	797:805	arg1	vaccines					810:817	vaccines	810:817	vaccines	810:817	The results indicate the prospects of using sulfated polysaccharides from F. evanescens as adjuvants in vaccines.					
32651818	4	6	dep	F.	780:781	arg1	evanescens					783:792	F. evanescens	780:792	F. evanescens	780:792	The results indicate the prospects of using sulfated polysaccharides from F. evanescens as adjuvants in vaccines.					
32651818	0	7	from	Effect	0:5	arg1	Marine					40:45	Marine	40:45	Marine	40:45	Effect of Sulfated Polysaccharides from Marine Hydrobionts on Humoral Immune Response to Ovalbumin in Mice.					
32651818	3	8	theme	native	577:582	arg1	fucoidans					610:618	native and structurally modified fucoidans	577:618	native and structurally modified fucoidans	577:618	The adjuvant effect of native and structurally modified fucoidans is slightly inferior to that of the traditional licensed aluminum hydroxide adjuvant.					
32651818	2	9	theme	native	298:303	arg1	fucoidan					305:312	native fucoidan	298:312	native fucoidan in combination with polyphenols	298:344	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	1	10	theme	fucoidans	129:137	arg1	activity					117:124	Adjuvant activity	108:124	Adjuvant activity of fucoidans in experimental vaccine compositions with ovalbumin	108:189	Adjuvant activity of fucoidans in experimental vaccine compositions with ovalbumin was studied on a mouse model.					
32651818	4	11	from	F.	780:781	arg1	polysaccharides					759:773	sulfated polysaccharides	750:773	sulfated polysaccharides from F. evanescens	750:792	The results indicate the prospects of using sulfated polysaccharides from F. evanescens as adjuvants in vaccines.					
32651818	4	11	from	F.	780:781	arg1	adjuvants					797:805	adjuvants	797:805	adjuvants in vaccines	797:817	The results indicate the prospects of using sulfated polysaccharides from F. evanescens as adjuvants in vaccines.					
32651818	2	12	theme	multiple	403:410	arg1	productions					412:422	multiple productions	403:422	multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a,	403:508	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	13	with	combination	317:327	arg1	polyphenols					334:344	polyphenols	334:344	polyphenols	334:344	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	0	14	from	Marine	40:45	arg1	Effect					0:5	Effect	0:5	Effect of Sulfated Polysaccharides from Marine	0:45	Effect of Sulfated Polysaccharides from Marine Hydrobionts on Humoral Immune Response to Ovalbumin in Mice.					
32651818	0	14	from	Marine	40:45	arg1	Polysaccharides					19:33	Sulfated Polysaccharides	10:33	Sulfated Polysaccharides from Marine	10:45	Effect of Sulfated Polysaccharides from Marine Hydrobionts on Humoral Immune Response to Ovalbumin in Mice.					
32651818	2	15	from	product	353:359	arg1	combination					317:327	combination	317:327	combination with polyphenols	317:344	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	4	16	theme	sulfated	750:757	arg1	polysaccharides					759:773	sulfated polysaccharides	750:773	sulfated polysaccharides from F. evanescens	750:792	The results indicate the prospects of using sulfated polysaccharides from F. evanescens as adjuvants in vaccines.					
32651818	4	16	theme	sulfated	750:757	arg1	adjuvants					797:805	adjuvants	797:805	adjuvants in vaccines	797:817	The results indicate the prospects of using sulfated polysaccharides from F. evanescens as adjuvants in vaccines.					
32651818	2	17	with	Compositions	221:232	arg1	polysaccharides					248:262	sulfated polysaccharides	239:262	sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis)	239:393	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	3	18	theme	licensed	668:675	arg1	aluminum					677:684	the traditional licensed aluminum hydroxide adjuvant	652:703	the traditional licensed aluminum hydroxide adjuvant	652:703	The adjuvant effect of native and structurally modified fucoidans is slightly inferior to that of the traditional licensed aluminum hydroxide adjuvant.					
32651818	2	19	theme	hydrolysis	383:392	arg1	product					353:359	a product	351:359	a product of fucoidan enzymatic hydrolysis	351:392	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	19	theme	hydrolysis	383:392	arg1	fucoidan					305:312	native fucoidan	298:312	native fucoidan in combination with polyphenols	298:344	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	20	theme	total	457:461	arg1	IgG					463:465	total IgG	457:465	total IgG	457:465	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	1	21	from	activity	117:124	arg1	compositions					163:174	experimental vaccine compositions	142:174	experimental vaccine compositions with ovalbumin	142:189	Adjuvant activity of fucoidans in experimental vaccine compositions with ovalbumin was studied on a mouse model.					
32651818	2	22	theme	antibodies	444:453	arg1	productions					412:422	multiple productions	403:422	multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a,	403:508	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	0	23	theme	Sulfated	10:17	arg1	Polysaccharides					19:33	Sulfated Polysaccharides	10:33	Sulfated Polysaccharides from Marine	10:45	Effect of Sulfated Polysaccharides from Marine Hydrobionts on Humoral Immune Response to Ovalbumin in Mice.					
32651818	3	24	theme	adjuvant	558:565	arg1	effect					567:572	The adjuvant effect	554:572	The adjuvant effect of native and structurally modified fucoidans	554:618	The adjuvant effect of native and structurally modified fucoidans is slightly inferior to that of the traditional licensed aluminum hydroxide adjuvant.					
32651818	3	24	theme	adjuvant	558:565	arg1	inferior					632:639	inferior	632:639	inferior	632:639	The adjuvant effect of native and structurally modified fucoidans is slightly inferior to that of the traditional licensed aluminum hydroxide adjuvant.					
32651818	2	25	from	alga	275:278	arg1	polysaccharides					248:262	sulfated polysaccharides	239:262	sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis)	239:393	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	26	theme	antigen-specific	427:442	arg1	isotypes					472:479	its isotypes	468:479	its isotypes IgG1 and, especially, IgG2a	468:507	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	26	theme	antigen-specific	427:442	arg1	antibodies					444:453	antigen-specific antibodies	427:453	antigen-specific antibodies	427:453	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	27	theme	individual	532:541	arg1	ovalbumin					543:551	an individual ovalbumin	529:551	an individual ovalbumin	529:551	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	28	theme	sulfated	239:246	arg1	polysaccharides					248:262	sulfated polysaccharides	239:262	sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis)	239:393	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	1	29	theme	mouse	208:212	arg1	model					214:218	a mouse model	206:218	a mouse model	206:218	Adjuvant activity of fucoidans in experimental vaccine compositions with ovalbumin was studied on a mouse model.					
32651818	0	30	theme	Polysaccharides	19:33	arg1	Effect					0:5	Effect	0:5	Effect of Sulfated Polysaccharides from Marine	0:45	Effect of Sulfated Polysaccharides from Marine Hydrobionts on Humoral Immune Response to Ovalbumin in Mice.					
32651818	2	31	dep	antibodies	444:453	arg1	IgG					463:465	total IgG	457:465	total IgG	457:465	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	1	32	theme	Adjuvant	108:115	arg1	activity					117:124	Adjuvant activity	108:124	Adjuvant activity of fucoidans in experimental vaccine compositions with ovalbumin	108:189	Adjuvant activity of fucoidans in experimental vaccine compositions with ovalbumin was studied on a mouse model.					
32651818	2	33	dep	evanescens	286:295	arg1	fucoidan					305:312	native fucoidan	298:312	native fucoidan in combination with polyphenols	298:344	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	33	dep	evanescens	286:295	arg1	product					353:359	a product	351:359	a product of fucoidan enzymatic hydrolysis	351:392	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	2	34	with	comparison	513:522	arg1	ovalbumin					543:551	an individual ovalbumin	529:551	an individual ovalbumin	529:551	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	3	35	theme	hydroxide	686:694	arg1	aluminum					677:684	the traditional licensed aluminum hydroxide adjuvant	652:703	the traditional licensed aluminum hydroxide adjuvant	652:703	The adjuvant effect of native and structurally modified fucoidans is slightly inferior to that of the traditional licensed aluminum hydroxide adjuvant.					
32651818	3	36	theme	modified	601:608	arg1	fucoidans					610:618	native and structurally modified fucoidans	577:618	native and structurally modified fucoidans	577:618	The adjuvant effect of native and structurally modified fucoidans is slightly inferior to that of the traditional licensed aluminum hydroxide adjuvant.					
32651818	3	37	theme	traditional	656:666	arg1	aluminum					677:684	the traditional licensed aluminum hydroxide adjuvant	652:703	the traditional licensed aluminum hydroxide adjuvant	652:703	The adjuvant effect of native and structurally modified fucoidans is slightly inferior to that of the traditional licensed aluminum hydroxide adjuvant.					
32651818	2	38	theme	brown	269:273	arg1	alga					275:278	brown alga	269:278	brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis)	269:393	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	1	39	theme	experimental	142:153	arg1	compositions					163:174	experimental vaccine compositions	142:174	experimental vaccine compositions with ovalbumin	142:189	Adjuvant activity of fucoidans in experimental vaccine compositions with ovalbumin was studied on a mouse model.					
32651818	0	40	theme	Immune	70:75	arg1	Response					77:84	Humoral Immune Response	62:84	Humoral Immune Response to Ovalbumin in Mice	62:105	Effect of Sulfated Polysaccharides from Marine Hydrobionts on Humoral Immune Response to Ovalbumin in Mice.					
32651818	0	41	from	Ovalbumin	89:97	arg1	Mice					102:105	Mice	102:105	Mice	102:105	Effect of Sulfated Polysaccharides from Marine Hydrobionts on Humoral Immune Response to Ovalbumin in Mice.					
32651818	3	42	theme	fucoidans	610:618	arg1	effect					567:572	The adjuvant effect	554:572	The adjuvant effect of native and structurally modified fucoidans	554:618	The adjuvant effect of native and structurally modified fucoidans is slightly inferior to that of the traditional licensed aluminum hydroxide adjuvant.					
32651818	3	42	theme	fucoidans	610:618	arg1	inferior					632:639	inferior	632:639	inferior	632:639	The adjuvant effect of native and structurally modified fucoidans is slightly inferior to that of the traditional licensed aluminum hydroxide adjuvant.					
32651818	2	43	theme	enzymatic	373:381	arg1	hydrolysis					383:392	fucoidan enzymatic hydrolysis	364:392	fucoidan enzymatic hydrolysis	364:392	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
32651818	0	44	theme	Humoral	62:68	arg1	Response					77:84	Humoral Immune Response	62:84	Humoral Immune Response to Ovalbumin in Mice	62:105	Effect of Sulfated Polysaccharides from Marine Hydrobionts on Humoral Immune Response to Ovalbumin in Mice.					
32651818	2	45	theme	fucoidan	364:371	arg1	hydrolysis					383:392	fucoidan enzymatic hydrolysis	364:392	fucoidan enzymatic hydrolysis	364:392	Compositions with sulfated polysaccharides from brown alga Fucus evanescens (native fucoidan in combination with polyphenols, and a product of fucoidan enzymatic hydrolysis) induced multiple productions of antigen-specific antibodies - total IgG, its isotypes IgG1 and, especially, IgG2a, in comparison with an individual ovalbumin.					
34906803	3	0	theme	chemical	629:636	arg1	structure					638:646	the chemical structure	625:646	the chemical structure	625:646	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	4	1	theme	food	784:787	arg1	sample					726:731	The best XH sample	714:731	The best XH sample with optimum XG concentration of 2 g/L	714:770	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	4	1	theme	food	784:787	arg1	packaging					789:797	food packaging	784:797	food packaging	784:797	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	0	2	with	film	45:48	arg1	gum					63:65	xanthan gum	55:65	xanthan gum	55:65	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	1	3	theme	hydroxypropyl	216:228	arg1	methylcellulose					230:244	hydroxypropyl methylcellulose	216:244	hydroxypropyl methylcellulose (HPMC)	216:251	A novel film composed of xanthan gum (XG) and hydroxypropyl methylcellulose (HPMC) was prepared (XH).					
34906803	1	3	theme	hydroxypropyl	216:228	arg1	HPMC					247:250	HPMC	247:250	HPMC	247:250	A novel film composed of xanthan gum (XG) and hydroxypropyl methylcellulose (HPMC) was prepared (XH).					
34906803	5	4	theme	flavor	978:983	arg1	substances					985:994	flavor substances	978:994	flavor substances	978:994	Consequently, the release of flavor substances was also decreased.					
34906803	5	5	theme	substances	985:994	arg1	release					967:973	the release	963:973	the release of flavor substances	963:994	Consequently, the release of flavor substances was also decreased.					
34906803	6	6	theme	packaging	1136:1144	arg1	applications					1146:1157	food packaging applications	1131:1157	food packaging applications	1131:1157	Banana shelf life has qualitatively improved with XH composite film for food preservation and affirmed the uses in food packaging applications.					
34906803	0	7	theme	excellent	93:101	arg1	bio-material					118:129	an excellent food packaging bio-material	90:129	an excellent food packaging bio-material in enhancing the shelf life of banana	90:167	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	6	8	theme	XH	1066:1067	arg1	film					1079:1082	XH composite film	1066:1082	XH composite film for food preservation	1066:1104	Banana shelf life has qualitatively improved with XH composite film for food preservation and affirmed the uses in food packaging applications.					
34906803	4	9	dep	%	896:896	arg1	coating					939:945	XH coating	936:945	XH coating	936:945	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	2	10	dep	transform	312:320	arg1	infrared					322:329	infrared	322:329	transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM)	312:415	The films were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
34906803	3	11	theme	crystalline	649:659	arg1	texture					661:667	crystalline texture	649:667	crystalline texture	649:667	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	3	12	theme	transmission	481:492	arg1	WVTR					500:503	WVTR	500:503	WVTR	500:503	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	3	12	theme	transmission	481:492	arg1	rate					494:497	water vapor transmission rate	469:497	water vapor transmission rate (WVTR)	469:504	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	2	13	dep	Fourier	304:310	arg1	transform					312:320	transform	312:320	transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM)	312:415	The films were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
34906803	4	14	from	rate	848:851	arg1	banana					856:861	banana	856:861	banana	856:861	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	0	15	theme	packaging	108:116	arg1	bio-material					118:129	an excellent food packaging bio-material	90:129	an excellent food packaging bio-material in enhancing the shelf life of banana	90:167	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	4	16	theme	2 g/L	766:770	arg1	concentration					749:761	optimum XG concentration	738:761	optimum XG concentration of 2 g/L	738:770	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	3	17	contain	had	597:599	arg2	effect					615:620	a significant effect	601:620	a significant effect	601:620	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	3	17	contain	had	597:599	arg1	transmittance					428:440	The light transmittance	418:440	The light transmittance	418:440	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	0	18	theme	food	103:106	arg1	bio-material					118:129	an excellent food packaging bio-material	90:129	an excellent food packaging bio-material in enhancing the shelf life of banana	90:167	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	4	19	with	sample	726:731	arg1	concentration					749:761	optimum XG concentration	738:761	optimum XG concentration of 2 g/L	738:770	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	3	20	theme	water	469:473	arg1	WVTR					500:503	WVTR	500:503	WVTR	500:503	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	3	20	theme	water	469:473	arg1	rate					494:497	water vapor transmission rate	469:497	water vapor transmission rate (WVTR)	469:504	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	2	21	theme	scanning	382:389	arg1	microscopy					400:409	scanning electron microscopy	382:409	scanning electron microscopy (SEM)	382:415	The films were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
34906803	2	21	theme	scanning	382:389	arg1	SEM					412:414	SEM	412:414	SEM	412:414	The films were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
34906803	6	22	dep	the	1119:1121	arg1	uses					1123:1126	uses	1123:1126	uses	1123:1126	Banana shelf life has qualitatively improved with XH composite film for food preservation and affirmed the uses in food packaging applications.					
34906803	6	23	theme	Banana	1016:1021	arg1	life					1029:1032	Banana shelf life	1016:1032	Banana shelf life	1016:1032	Banana shelf life has qualitatively improved with XH composite film for food preservation and affirmed the uses in food packaging applications.					
34906803	1	24	theme	novel	172:176	arg1	film					178:181	A novel film	170:181	A novel film composed of xanthan gum (XG) and hydroxypropyl methylcellulose (HPMC)	170:251	A novel film composed of xanthan gum (XG) and hydroxypropyl methylcellulose (HPMC) was prepared (XH).					
34906803	4	25	theme	XH	907:908	arg1	coating					910:916	XH coating	907:916	XH coating	907:916	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	0	26	theme	xanthan	55:61	arg1	gum					63:65	xanthan gum	55:65	xanthan gum	55:65	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	0	27	theme	methylcellulose	29:43	arg1	film					45:48	hydroxypropyl methylcellulose film	15:48	hydroxypropyl methylcellulose film with xanthan gum	15:65	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	4	28	theme	optimum	738:744	arg1	concentration					749:761	optimum XG concentration	738:761	optimum XG concentration of 2 g/L	738:770	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	0	29	theme	hydroxypropyl	15:27	arg1	film					45:48	hydroxypropyl methylcellulose film	15:48	hydroxypropyl methylcellulose film with xanthan gum	15:65	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	4	30	theme	loss	843:846	arg1	rate					848:851	the weight loss rate	832:851	the weight loss rate on banana	832:861	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	4	30	theme	loss	843:846	arg1	able					867:870	able	867:870	able	867:870	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	3	31	theme	good	520:523	arg1	compatibility					525:537	the good compatibility	516:537	the good compatibility between XG and HPMC	516:557	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	1	32	theme	xanthan	195:201	arg1	XG					208:209	XG	208:209	XG	208:209	A novel film composed of xanthan gum (XG) and hydroxypropyl methylcellulose (HPMC) was prepared (XH).					
34906803	1	32	theme	xanthan	195:201	arg1	gum					203:205	xanthan gum	195:205	xanthan gum (XG)	195:210	A novel film composed of xanthan gum (XG) and hydroxypropyl methylcellulose (HPMC) was prepared (XH).					
34906803	0	33	theme	film	45:48	arg1	application					75:85	its application	71:85	its application as an excellent food packaging bio-material in enhancing the shelf life of banana	71:167	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	0	33	theme	film	45:48	arg1	Development					0:10	Development	0:10	Development of hydroxypropyl methylcellulose film with xanthan gum	0:65	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	3	34	theme	film	708:711	arg1	microstructure					673:686	microstructure	673:686	microstructure	673:686	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	3	34	theme	film	708:711	arg1	structure					638:646	the chemical structure	625:646	the chemical structure	625:646	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	3	34	theme	film	708:711	arg1	texture					661:667	crystalline texture	649:667	crystalline texture	649:667	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	4	35	theme	weight	836:841	arg1	rate					848:851	the weight loss rate	832:851	the weight loss rate on banana	832:861	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	4	35	theme	weight	836:841	arg1	able					867:870	able	867:870	able	867:870	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	6	36	theme	food	1088:1091	arg1	preservation					1093:1104	food preservation	1088:1104	food preservation	1088:1104	Banana shelf life has qualitatively improved with XH composite film for food preservation and affirmed the uses in food packaging applications.					
34906803	2	37	theme	X-ray	353:357	arg1	diffraction					359:369	X-ray diffraction	353:369	X-ray diffraction (XRD)	353:375	The films were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
34906803	2	37	theme	X-ray	353:357	arg1	XRD					372:374	XRD	372:374	XRD	372:374	The films were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
34906803	4	38	used	used	776:779	arg2	packaging					789:797	food packaging	784:797	food packaging	784:797	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	4	38	used	used	776:779	arg2	sample					726:731	The best XH sample	714:731	The best XH sample with optimum XG concentration of 2 g/L	714:770	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	3	39	theme	hydrogen-bond	564:576	arg1	interaction					578:588	hydrogen-bond interaction	564:588	hydrogen-bond interaction	564:588	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	2	40	theme	electron	391:398	arg1	microscopy					400:409	scanning electron microscopy	382:409	scanning electron microscopy (SEM)	382:415	The films were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
34906803	2	40	theme	electron	391:398	arg1	SEM					412:414	SEM	412:414	SEM	412:414	The films were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM).					
34906803	3	41	theme	XH	695:696	arg1	film					708:711	the XH composite film	691:711	the XH composite film	691:711	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	0	42	theme	shelf	148:152	arg1	life					154:157	the shelf life	144:157	the shelf life of banana	144:167	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	4	43	theme	XG	746:747	arg1	concentration					749:761	optimum XG concentration	738:761	optimum XG concentration of 2 g/L	738:770	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	3	44	theme	light	422:426	arg1	transmittance					428:440	The light transmittance	418:440	The light transmittance	418:440	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	3	45	theme	composite	698:706	arg1	film					708:711	the XH composite film	691:711	the XH composite film	691:711	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	0	46	theme	banana	162:167	arg1	life					154:157	the shelf life	144:157	the shelf life of banana	144:167	Development of hydroxypropyl methylcellulose film with xanthan gum and its application as an excellent food packaging bio-material in enhancing the shelf life of banana.					
34906803	3	47	theme	significant	603:613	arg1	effect					615:620	a significant effect	601:620	a significant effect	601:620	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	6	48	theme	composite	1069:1077	arg1	film					1079:1082	XH composite film	1066:1082	XH composite film for food preservation	1066:1104	Banana shelf life has qualitatively improved with XH composite film for food preservation and affirmed the uses in food packaging applications.					
34906803	4	49	theme	XH	936:937	arg1	coating					939:945	XH coating	936:945	XH coating	936:945	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	6	50	theme	shelf	1023:1027	arg1	life					1029:1032	Banana shelf life	1016:1032	Banana shelf life	1016:1032	Banana shelf life has qualitatively improved with XH composite film for food preservation and affirmed the uses in food packaging applications.					
34906803	3	51	theme	vapor	475:479	arg1	WVTR					500:503	WVTR	500:503	WVTR	500:503	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	3	51	theme	vapor	475:479	arg1	rate					494:497	water vapor transmission rate	469:497	water vapor transmission rate (WVTR)	469:504	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	3	52	theme	mechanical	443:452	arg1	properties					454:463	mechanical properties	443:463	mechanical properties	443:463	The light transmittance, mechanical properties and water vapor transmission rate (WVTR) indicated the good compatibility between XG and HPMC with hydrogen-bond interaction and XG had a significant effect on the chemical structure, crystalline texture and microstructure of the XH composite film.					
34906803	1	53	dep	prepared	257:264	arg1	XH					267:268	XH	267:268	XH	267:268	A novel film composed of xanthan gum (XG) and hydroxypropyl methylcellulose (HPMC) was prepared (XH).					
34906803	4	54	theme	XH	723:724	arg1	sample					726:731	The best XH sample	714:731	The best XH sample with optimum XG concentration of 2 g/L	714:770	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	4	54	theme	XH	723:724	arg1	packaging					789:797	food packaging	784:797	food packaging	784:797	The best XH sample with optimum XG concentration of 2 g/L was used as food packaging via coating onto banana, whereby the weight loss rate on banana was able to decreased from 25 ± 3% (without XH coating) to 16 ± 4% (with XH coating).					
34906803	6	55	theme	food	1131:1134	arg1	applications					1146:1157	food packaging applications	1131:1157	food packaging applications	1131:1157	Banana shelf life has qualitatively improved with XH composite film for food preservation and affirmed the uses in food packaging applications.					
33541638	0	0	theme	novel	69:73	arg1	glucan					75:80	a novel glucan	67:80	a novel glucan	67:80	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	2	1	theme	α-d-Glcp	361:368	arg1	residues					373:380	α-d-Glcp(1→ residues	361:380	α-d-Glcp(1→ residues	361:380	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	5	2	theme	particle	531:538	arg1	size					540:543	Its particle size	527:543	Its particle size	527:543	Its particle size was mainly concentrated at 230 nm in water.					
33541638	9	3	theme	structural	1023:1032	arg1	characteristics					1034:1048	the structural characteristics	1019:1048	the structural characteristics of sweet potato polysaccharides	1019:1080	Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.					
33541638	9	4	from	characteristics	1034:1048	arg1	foods					1108:1112	foods	1108:1112	foods	1108:1112	Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.					
33541638	9	4	from	characteristics	1034:1048	arg1	areas					1133:1137	pharmaceutical areas	1118:1137	pharmaceutical areas	1118:1137	Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.					
33541638	6	5	dep	showed	634:639	arg1	conformation					667:678	no triple-helix conformation	651:678	no triple-helix conformation	651:678	Congo red and circular dichroism experiments showed there was no triple-helix conformation.					
33541638	0	6	from	Lam	132:134	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	0	6	from	Lam	132:134	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	0	6	from	Lam	132:134	arg1	activity					55:62	in vitro antitumor activity	36:62	in vitro antitumor activity	36:62	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	2	7	from	O-3	333:335	arg1	glycosyl					297:304	→4)-α-d-Glcp(1→ glycosyl, and branching	281:319	glycosyl	297:304	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	2	7	from	O-3	333:335	arg1	branching					311:319	→4)-α-d-Glcp(1→ glycosyl, and branching	281:319	branching	311:319	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	7	8	theme	chain	834:838	arg1	length					840:845	chain length	834:845	chain length	834:845	Atomic force microscopy data suggested that its height and width ranged from 1.0 to 6.1 nm and 65 to 210 nm, respectively; its maximum ring diameter and chain length was ∼800 nm and ∼7.0 μm, respectively.					
33541638	7	9	dep	6.1 nm	765:770	arg1	to					762:763	to	762:763	to	762:763	Atomic force microscopy data suggested that its height and width ranged from 1.0 to 6.1 nm and 65 to 210 nm, respectively; its maximum ring diameter and chain length was ∼800 nm and ∼7.0 μm, respectively.					
33541638	8	10	theme	inhibitory	912:921	arg1	activities					923:932	inhibitory activities	912:932	inhibitory activities on HepG2, LOVO, and MCF-7 cells	912:964	Furthermore, it exhibited inhibitory activities on HepG2, LOVO, and MCF-7 cells.					
33541638	7	11	dep	210 nm	782:787	arg1	to					779:780	to	779:780	to	779:780	Atomic force microscopy data suggested that its height and width ranged from 1.0 to 6.1 nm and 65 to 210 nm, respectively; its maximum ring diameter and chain length was ∼800 nm and ∼7.0 μm, respectively.					
33541638	0	12	theme	glucan	75:80	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	0	12	theme	glucan	75:80	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	0	12	theme	glucan	75:80	arg1	activity					55:62	in vitro antitumor activity	36:62	in vitro antitumor activity	36:62	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	9	13	theme	sweet	1053:1057	arg1	polysaccharides					1066:1080	sweet potato polysaccharides	1053:1080	sweet potato polysaccharides	1053:1080	Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.					
33541638	6	14	theme	red	595:597	arg1	experiments					622:632	Congo red and circular dichroism experiments	589:632	Congo red and circular dichroism experiments	589:632	Congo red and circular dichroism experiments showed there was no triple-helix conformation.					
33541638	1	15	theme	purple	206:211	arg1	Lam					247:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	0	16	theme	purple	91:96	arg1	Lam					132:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	9	17	theme	potato	1059:1064	arg1	polysaccharides					1066:1080	sweet potato polysaccharides	1053:1080	sweet potato polysaccharides	1053:1080	Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.					
33541638	6	18	theme	Congo	589:593	arg1	experiments					622:632	Congo red and circular dichroism experiments	589:632	Congo red and circular dichroism experiments	589:632	Congo red and circular dichroism experiments showed there was no triple-helix conformation.					
33541638	2	19	from	positions	346:354	arg1	glycosyl					297:304	→4)-α-d-Glcp(1→ glycosyl, and branching	281:319	glycosyl	297:304	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	2	19	from	positions	346:354	arg1	branching					311:319	→4)-α-d-Glcp(1→ glycosyl, and branching	281:319	branching	311:319	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	1	20	theme	foot	192:195	arg1	tuber					197:201	the foot tuber	188:201	the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam	188:249	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	7	21	theme	maximum	808:814	arg1	diameter					821:828	its maximum ring diameter	804:828	its maximum ring diameter	804:828	Atomic force microscopy data suggested that its height and width ranged from 1.0 to 6.1 nm and 65 to 210 nm, respectively; its maximum ring diameter and chain length was ∼800 nm and ∼7.0 μm, respectively.					
33541638	2	22	theme	1→	294:295	arg1	glycosyl					297:304	→4)-α-d-Glcp(1→ glycosyl, and branching	281:319	glycosyl	297:304	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	7	23	theme	microscopy	694:703	arg1	data					705:708	Atomic force microscopy data	681:708	Atomic force microscopy data	681:708	Atomic force microscopy data suggested that its height and width ranged from 1.0 to 6.1 nm and 65 to 210 nm, respectively; its maximum ring diameter and chain length was ∼800 nm and ∼7.0 μm, respectively.					
33541638	7	24	theme	ring	816:819	arg1	diameter					821:828	its maximum ring diameter	804:828	its maximum ring diameter	804:828	Atomic force microscopy data suggested that its height and width ranged from 1.0 to 6.1 nm and 65 to 210 nm, respectively; its maximum ring diameter and chain length was ∼800 nm and ∼7.0 μm, respectively.					
33541638	1	25	theme	novel	139:143	arg1	18.3 kDa					160:167	18.3 kDa	160:167	18.3 kDa	160:167	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	1	25	theme	novel	139:143	arg1	PSPP-1					152:157	A novel glucan PSPP-1	137:157	A novel glucan PSPP-1 (18.3 kDa)	137:168	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	8	26	theme	MCF-7	954:958	arg1	cells					960:964	MCF-7 cells	954:964	MCF-7 cells	954:964	Furthermore, it exhibited inhibitory activities on HepG2, LOVO, and MCF-7 cells.					
33541638	1	27	theme	sweet	213:217	arg1	Lam					247:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	3	28	theme	amorphous	445:453	arg1	form					455:458	an amorphous form	442:458	an amorphous form	442:458	X-ray diffraction experiment showed that PSPP-1 existed as an amorphous form.					
33541638	1	29	theme	glucan	145:150	arg1	18.3 kDa					160:167	18.3 kDa	160:167	18.3 kDa	160:167	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	1	29	theme	glucan	145:150	arg1	PSPP-1					152:157	A novel glucan PSPP-1	137:157	A novel glucan PSPP-1 (18.3 kDa)	137:168	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	2	30	theme	O-2	328:330	arg1	O-3					333:335	the O-2, O-3, and O-6 positions	324:354	O-3	333:335	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	1	31	theme	potato	219:224	arg1	Lam					247:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	0	32	theme	potato	104:109	arg1	Lam					132:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	6	33	theme	triple-helix	654:665	arg1	conformation					667:678	no triple-helix conformation	651:678	no triple-helix conformation	651:678	Congo red and circular dichroism experiments showed there was no triple-helix conformation.					
33541638	7	34	theme	force	688:692	arg1	microscopy					694:703	Atomic force microscopy	681:703	Atomic force microscopy data	681:708	Atomic force microscopy data suggested that its height and width ranged from 1.0 to 6.1 nm and 65 to 210 nm, respectively; its maximum ring diameter and chain length was ∼800 nm and ∼7.0 μm, respectively.					
33541638	1	35	theme	Ipomoea	226:232	arg1	Lam					247:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	0	36	theme	sweet	98:102	arg1	Lam					132:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	4	37	theme	electron	506:513	arg1	microscopy					515:524	scanning electron microscopy	497:524	scanning electron microscopy	497:524	Its microstructure was detected via scanning electron microscopy.					
33541638	0	38	theme	in	36:37	arg1	activity					55:62	in vitro antitumor activity	36:62	in vitro antitumor activity	36:62	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	1	39	theme	Batatas	234:240	arg1	Lam					247:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	0	40	theme	Batatas	119:125	arg1	Lam					132:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	2	41	theme	-α-d-Glcp	284:292	arg1	glycosyl					297:304	→4)-α-d-Glcp(1→ glycosyl, and branching	281:319	glycosyl	297:304	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	2	42	theme	O-6	342:344	arg1	positions					346:354	the O-2, O-3, and O-6 positions	324:354	positions	346:354	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	0	43	theme	Ipomoea	111:117	arg1	Lam					132:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	8	44	from	activities	923:932	arg1	LOVO					944:947	LOVO	944:947	LOVO	944:947	Furthermore, it exhibited inhibitory activities on HepG2, LOVO, and MCF-7 cells.					
33541638	8	44	from	activities	923:932	arg1	HepG2					937:941	HepG2	937:941	HepG2	937:941	Furthermore, it exhibited inhibitory activities on HepG2, LOVO, and MCF-7 cells.					
33541638	8	44	from	activities	923:932	arg1	cells					960:964	MCF-7 cells	954:964	MCF-7 cells	954:964	Furthermore, it exhibited inhibitory activities on HepG2, LOVO, and MCF-7 cells.					
33541638	0	45	theme	antitumor	45:53	arg1	activity					55:62	in vitro antitumor activity	36:62	in vitro antitumor activity	36:62	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	1	46	theme	L.	243:244	arg1	Lam					247:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	purple sweet potato Ipomoea Batatas (L.) Lam	206:249	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	0	47	theme	L.	128:129	arg1	Lam					132:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	the purple sweet potato Ipomoea Batatas (L.) Lam	87:134	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	0	48	dep	in	36:37	arg1	vitro					39:43	vitro	39:43	vitro	39:43	Purification, characterization, and in vitro antitumor activity of a novel glucan from the purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	9	49	theme	pharmaceutical	1118:1131	arg1	areas					1133:1137	pharmaceutical areas	1118:1137	pharmaceutical areas	1118:1137	Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.					
33541638	2	50	with	O-3	333:335	arg1	residues					373:380	α-d-Glcp(1→ residues	361:380	α-d-Glcp(1→ residues	361:380	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	1	51	theme	Lam	247:249	arg1	tuber					197:201	the foot tuber	188:201	the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam	188:249	A novel glucan PSPP-1 (18.3 kDa) was purified from the foot tuber of purple sweet potato Ipomoea Batatas (L.) Lam.					
33541638	9	52	theme	polysaccharides	1066:1080	arg1	characteristics					1034:1048	the structural characteristics	1019:1048	the structural characteristics of sweet potato polysaccharides	1019:1080	Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.					
33541638	9	52	theme	polysaccharides	1066:1080	arg1	application					1093:1103	their application	1087:1103	their application in foods and pharmaceutical areas	1087:1137	Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.					
33541638	6	53	theme	circular	603:610	arg1	experiments					622:632	Congo red and circular dichroism experiments	589:632	Congo red and circular dichroism experiments	589:632	Congo red and circular dichroism experiments showed there was no triple-helix conformation.					
33541638	5	54	from	230 nm	572:577	arg1	water					582:586	water	582:586	water	582:586	Its particle size was mainly concentrated at 230 nm in water.					
33541638	2	55	theme	1→	370:371	arg1	residues					373:380	α-d-Glcp(1→ residues	361:380	α-d-Glcp(1→ residues	361:380	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	6	56	theme	dichroism	612:620	arg1	experiments					622:632	Congo red and circular dichroism experiments	589:632	Congo red and circular dichroism experiments	589:632	Congo red and circular dichroism experiments showed there was no triple-helix conformation.					
33541638	3	57	theme	X-ray	383:387	arg1	experiment					401:410	X-ray diffraction experiment	383:410	X-ray diffraction experiment	383:410	X-ray diffraction experiment showed that PSPP-1 existed as an amorphous form.					
33541638	4	58	theme	scanning	497:504	arg1	microscopy					515:524	scanning electron microscopy	497:524	scanning electron microscopy	497:524	Its microstructure was detected via scanning electron microscopy.					
33541638	2	59	with	positions	346:354	arg1	residues					373:380	α-d-Glcp(1→ residues	361:380	α-d-Glcp(1→ residues	361:380	Its backbone was composed of →4)-α-d-Glcp(1→ glycosyl, and branching at the O-2, O-3, and O-6 positions with α-d-Glcp(1→ residues.					
33541638	3	60	theme	diffraction	389:399	arg1	experiment					401:410	X-ray diffraction experiment	383:410	X-ray diffraction experiment	383:410	X-ray diffraction experiment showed that PSPP-1 existed as an amorphous form.					
33541638	9	61	from	application	1093:1103	arg1	foods					1108:1112	foods	1108:1112	foods	1108:1112	Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.					
33541638	9	61	from	application	1093:1103	arg1	areas					1133:1137	pharmaceutical areas	1118:1137	pharmaceutical areas	1118:1137	Collectively, our data are useful for understanding the structural characteristics of sweet potato polysaccharides, and their application in foods and pharmaceutical areas.					
33541638	7	62	theme	Atomic	681:686	arg1	microscopy					694:703	Atomic force microscopy	681:703	Atomic force microscopy data	681:708	Atomic force microscopy data suggested that its height and width ranged from 1.0 to 6.1 nm and 65 to 210 nm, respectively; its maximum ring diameter and chain length was ∼800 nm and ∼7.0 μm, respectively.					
33952698	7	0	theme	glycoprotein	1161:1172	arg1	type					1153:1156	type	1153:1156	type	1153:1156	The composition of these heterooligomers is dependent on the concentration of Gal-3 as well as on the concentration and type of glycoprotein.					
33952698	7	0	theme	glycoprotein	1161:1172	arg1	concentration					1135:1147	concentration	1135:1147	concentration	1135:1147	The composition of these heterooligomers is dependent on the concentration of Gal-3 as well as on the concentration and type of glycoprotein.					
33952698	11	1	theme	polymorphisms	1781:1793	arg1	role					1765:1768	the role	1761:1768	the role of proline polymorphisms (e.g., at P64) associated with many diseases	1761:1838	Our results unveil the role of proline polymorphisms (e.g., at P64) associated with many diseases and suggest that the function of glycosylated cell surface receptors is dynamically regulated by Gal-3.					
33952698	8	2	theme	disrupted	1265:1273	arg1	endomembranes					1275:1287	disrupted endomembranes	1265:1287	disrupted endomembranes	1265:1287	LLPS-like Gal-3 oligomerization/condensation was also observed on the plasma membrane and disrupted endomembranes.					
33952698	9	3	theme	Gal-3	1362:1366	arg1	LLPS					1368:1371	glycan binding-triggered Gal-3 LLPS	1337:1371	glycan binding-triggered Gal-3 LLPS (or LLPS-like)	1337:1386	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	1	4	theme	dynamic	159:165	arg1	tail					191:194	a long, aperiodic, and dynamic proline-rich N-terminal tail	136:194	a long, aperiodic, and dynamic proline-rich N-terminal tail (NT)	136:199	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	1	4	theme	dynamic	159:165	arg1	NT					197:198	NT	197:198	NT	197:198	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	9	5	theme	recognition	1486:1496	arg1	CRD					1506:1508	CRD	1506:1508	CRD	1506:1508	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	9	5	theme	recognition	1486:1496	arg1	domain					1498:1503	the carbohydrate recognition domain	1469:1503	the carbohydrate recognition domain (CRD)	1469:1509	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	11	6	theme	proline	1773:1779	arg1	polymorphisms					1781:1793	proline polymorphisms	1773:1793	proline polymorphisms (e.g., at P64) associated with many diseases	1773:1838	Our results unveil the role of proline polymorphisms (e.g., at P64) associated with many diseases and suggest that the function of glycosylated cell surface receptors is dynamically regulated by Gal-3.					
33952698	1	7	contain	has	132:134	arg1	Gal-3					125:129	Gal-3	125:129	Gal-3	125:129	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	1	7	contain	has	132:134	arg2	NT					197:198	NT	197:198	NT	197:198	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	1	7	contain	has	132:134	arg1	Galectin-3					113:122	Galectin-3	113:122	Galectin-3 (Gal-3)	113:130	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	1	7	contain	has	132:134	arg2	tail					191:194	a long, aperiodic, and dynamic proline-rich N-terminal tail	136:194	a long, aperiodic, and dynamic proline-rich N-terminal tail (NT)	136:199	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	11	8	theme	surface	1891:1897	arg1	receptors					1899:1907	glycosylated cell surface receptors	1873:1907	glycosylated cell surface receptors	1873:1907	Our results unveil the role of proline polymorphisms (e.g., at P64) associated with many diseases and suggest that the function of glycosylated cell surface receptors is dynamically regulated by Gal-3.					
33952698	9	9	theme	NT-NT	1528:1532	arg1	interactions					1534:1545	NT-NT interactions	1528:1545	NT-NT interactions	1528:1545	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	9	10	theme	lesser	1573:1578	arg1	extent					1580:1585	a lesser extent	1571:1585	a lesser extent	1571:1585	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	9	11	theme	dynamic	1408:1414	arg1	interactions					1431:1442	dynamic intermolecular interactions	1408:1442	dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face	1408:1516	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	9	12	theme	Molecular-	1290:1299	arg1	assays					1316:1321	Molecular- and cell-based assays	1290:1321	Molecular- and cell-based assays	1290:1321	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	8	13	located	observed	1229:1236	arg1	endomembranes					1275:1287	disrupted endomembranes	1265:1287	disrupted endomembranes	1265:1287	LLPS-like Gal-3 oligomerization/condensation was also observed on the plasma membrane and disrupted endomembranes.					
33952698	8	13	located	observed	1229:1236	arg2	oligomerization/condensation					1191:1218	LLPS-like Gal-3 oligomerization/condensation	1175:1218	LLPS-like Gal-3 oligomerization/condensation	1175:1218	LLPS-like Gal-3 oligomerization/condensation was also observed on the plasma membrane and disrupted endomembranes.					
33952698	8	13	located	observed	1229:1236	arg1	membrane					1252:1259	the plasma membrane	1241:1259	the plasma membrane	1241:1259	LLPS-like Gal-3 oligomerization/condensation was also observed on the plasma membrane and disrupted endomembranes.					
33952698	10	14	theme	proline	1605:1611	arg1	Mutation					1588:1595	Mutation	1588:1595	Mutation of each proline within the NT	1588:1625	Mutation of each proline within the NT differentially controls NT-CRD interactions, consequently affecting glycan binding, LLPS, and cellular activities.					
33952698	9	15	theme	intermolecular	1416:1429	arg1	interactions					1431:1442	dynamic intermolecular interactions	1408:1442	dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face	1408:1516	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	4	16	theme	cell	665:668	arg1	migration					670:678	cell migration	665:678	cell migration	665:678	Our findings show that mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A) dramatically and differentially inhibits Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination).					
33952698	1	17	theme	proline-rich	167:178	arg1	tail					191:194	a long, aperiodic, and dynamic proline-rich N-terminal tail	136:194	a long, aperiodic, and dynamic proline-rich N-terminal tail (NT)	136:199	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	1	17	theme	proline-rich	167:178	arg1	NT					197:198	NT	197:198	NT	197:198	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	8	18	theme	LLPS-like	1175:1183	arg1	oligomerization/condensation					1191:1218	LLPS-like Gal-3 oligomerization/condensation	1175:1218	LLPS-like Gal-3 oligomerization/condensation	1175:1218	LLPS-like Gal-3 oligomerization/condensation was also observed on the plasma membrane and disrupted endomembranes.					
33952698	4	19	dep	proline	526:532	arg1	P55A					552:555	P55A	552:555	P55A	552:555	Our findings show that mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A) dramatically and differentially inhibits Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination).					
33952698	4	19	dep	proline	526:532	arg1	P64A/H					564:569	P64A/H	564:569	P64A/H	564:569	Our findings show that mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A) dramatically and differentially inhibits Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination).					
33952698	4	19	dep	proline	526:532	arg1	P67A					576:579	P67A	576:579	P67A	576:579	Our findings show that mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A) dramatically and differentially inhibits Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination).					
33952698	7	20	theme	Gal-3	1111:1115	arg1	concentration					1094:1106	the concentration	1090:1106	the concentration of Gal-3	1090:1115	The composition of these heterooligomers is dependent on the concentration of Gal-3 as well as on the concentration and type of glycoprotein.					
33952698	10	21	theme	glycan	1695:1700	arg1	binding					1702:1708	glycan binding	1695:1708	glycan binding	1695:1708	Mutation of each proline within the NT differentially controls NT-CRD interactions, consequently affecting glycan binding, LLPS, and cellular activities.					
33952698	1	22	theme	N-terminal	180:189	arg1	tail					191:194	a long, aperiodic, and dynamic proline-rich N-terminal tail	136:194	a long, aperiodic, and dynamic proline-rich N-terminal tail (NT)	136:199	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	1	22	theme	N-terminal	180:189	arg1	NT					197:198	NT	197:198	NT	197:198	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	0	23	theme	N-terminal	11:20	arg1	prolines					27:34	Galectin-3 N-terminal tail prolines	0:34	Galectin-3 N-terminal tail prolines	0:34	Galectin-3 N-terminal tail prolines modulate cell activity and glycan-mediated oligomerization/phase separation.					
33952698	9	24	dep	LLPS	1368:1371	arg1	LLPS-like					1377:1385	LLPS-like	1377:1385	LLPS-like	1377:1385	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	4	25	theme	proline	526:532	arg1	mutation					503:510	mutation	503:510	mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A)	503:580	Our findings show that mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A) dramatically and differentially inhibits Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination).					
33952698	4	26	theme	Gal-3-mediated	623:636	arg1	activities					647:656	Gal-3-mediated cellular activities	623:656	Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination)	623:726	Our findings show that mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A) dramatically and differentially inhibits Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination).					
33952698	0	27	theme	Galectin-3	0:9	arg1	prolines					27:34	Galectin-3 N-terminal tail prolines	0:34	Galectin-3 N-terminal tail prolines	0:34	Galectin-3 N-terminal tail prolines modulate cell activity and glycan-mediated oligomerization/phase separation.					
33952698	2	28	with	role	217:220	arg1	prolines					250:257	its numerous prolines	237:257	its numerous prolines	237:257	The functional role of the NT with its numerous prolines has remained enigmatic since its discovery.					
33952698	4	29	dep	migration	670:678	arg1	i.e.					659:662	i.e.	659:662	i.e.	659:662	Our findings show that mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A) dramatically and differentially inhibits Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination).					
33952698	3	30	theme	first	398:402	arg1	residues					407:414	the first 68 residues	394:414	the first 68 residues	394:414	To provide some resolution to this puzzle, we individually mutated all 14 NT prolines over the first 68 residues and assessed their effects on various Gal-3-mediated functions.					
33952698	4	31	theme	single	519:524	arg1	proline					526:532	any single proline	515:532	any single proline (especially P37A, P55A, P60A, P64A/H, and P67A)	515:580	Our findings show that mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A) dramatically and differentially inhibits Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination).					
33952698	10	32	theme	cellular	1721:1728	arg1	activities					1730:1739	cellular activities	1721:1739	cellular activities	1721:1739	Mutation of each proline within the NT differentially controls NT-CRD interactions, consequently affecting glycan binding, LLPS, and cellular activities.					
33952698	11	33	theme	many	1826:1829	arg1	diseases					1831:1838	many diseases	1826:1838	many diseases	1826:1838	Our results unveil the role of proline polymorphisms (e.g., at P64) associated with many diseases and suggest that the function of glycosylated cell surface receptors is dynamically regulated by Gal-3.					
33952698	7	34	theme	heterooligomers	1058:1072	arg1	dependent					1077:1085	dependent	1077:1085	dependent	1077:1085	The composition of these heterooligomers is dependent on the concentration of Gal-3 as well as on the concentration and type of glycoprotein.					
33952698	7	34	theme	heterooligomers	1058:1072	arg1	composition					1037:1047	The composition	1033:1047	The composition of these heterooligomers	1033:1072	The composition of these heterooligomers is dependent on the concentration of Gal-3 as well as on the concentration and type of glycoprotein.					
33952698	0	35	theme	tail	22:25	arg1	prolines					27:34	Galectin-3 N-terminal tail prolines	0:34	Galectin-3 N-terminal tail prolines	0:34	Galectin-3 N-terminal tail prolines modulate cell activity and glycan-mediated oligomerization/phase separation.					
33952698	9	36	theme	glycan	1337:1342	arg1	LLPS					1368:1371	glycan binding-triggered Gal-3 LLPS	1337:1371	glycan binding-triggered Gal-3 LLPS (or LLPS-like)	1337:1386	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	7	37	gly	glycoprotein	1161:1172	arg1	glycoprotein					1161:1172	glycoprotein	1161:1172	glycoprotein	1161:1172	The composition of these heterooligomers is dependent on the concentration of Gal-3 as well as on the concentration and type of glycoprotein.					
33952698	5	38	theme	prolines	782:789	arg1	role					774:777	the role	770:777	the role of prolines in mediating Gal-3 oligomerization, a fundamental process required for these cell activities	770:882	For mechanistic insight, we investigated the role of prolines in mediating Gal-3 oligomerization, a fundamental process required for these cell activities.					
33952698	0	39	theme	cell	45:48	arg1	activity					50:57	cell activity	45:57	cell activity	45:57	Galectin-3 N-terminal tail prolines modulate cell activity and glycan-mediated oligomerization/phase separation.					
33952698	9	40	theme	binding-triggered	1344:1360	arg1	LLPS					1368:1371	glycan binding-triggered Gal-3 LLPS	1337:1371	glycan binding-triggered Gal-3 LLPS (or LLPS-like)	1337:1386	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	11	41	theme	receptors	1899:1907	arg1	function					1861:1868	the function	1857:1868	the function of glycosylated cell surface receptors	1857:1907	Our results unveil the role of proline polymorphisms (e.g., at P64) associated with many diseases and suggest that the function of glycosylated cell surface receptors is dynamically regulated by Gal-3.					
33952698	9	42	theme	Gal-3	1456:1460	arg1	NT					1462:1463	the Gal-3 NT	1452:1463	the Gal-3 NT	1452:1463	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	6	43	theme	phase	1008:1012	arg1	separation					1014:1023	liquid-liquid phase separation	994:1023	liquid-liquid phase separation (LLPS)	994:1030	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
33952698	6	43	theme	phase	1008:1012	arg1	LLPS					1026:1029	LLPS	1026:1029	LLPS	1026:1029	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
33952698	5	44	theme	Gal-3	804:808	arg1	oligomerization					810:824	Gal-3 oligomerization	804:824	Gal-3 oligomerization	804:824	For mechanistic insight, we investigated the role of prolines in mediating Gal-3 oligomerization, a fundamental process required for these cell activities.					
33952698	5	44	theme	Gal-3	804:808	arg1	process					841:847	a fundamental process	827:847	a fundamental process required for these cell activities	827:882	For mechanistic insight, we investigated the role of prolines in mediating Gal-3 oligomerization, a fundamental process required for these cell activities.					
33952698	6	45	theme	Gal-3	900:904	arg1	process					973:979	a dynamic process	963:979	a dynamic process analogous to liquid-liquid phase separation (LLPS)	963:1030	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
33952698	6	45	theme	Gal-3	900:904	arg1	oligomerization					906:920	Gal-3 oligomerization	900:920	Gal-3 oligomerization triggered by binding to glycoproteins	900:958	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
33952698	5	46	theme	mechanistic	733:743	arg1	insight					745:751	mechanistic insight	733:751	mechanistic insight	733:751	For mechanistic insight, we investigated the role of prolines in mediating Gal-3 oligomerization, a fundamental process required for these cell activities.					
33952698	9	47	theme	carbohydrate	1473:1484	arg1	CRD					1506:1508	CRD	1506:1508	CRD	1506:1508	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	9	47	theme	carbohydrate	1473:1484	arg1	domain					1498:1503	the carbohydrate recognition domain	1469:1503	the carbohydrate recognition domain (CRD)	1469:1509	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	6	48	theme	liquid-liquid	994:1006	arg1	separation					1014:1023	liquid-liquid phase separation	994:1023	liquid-liquid phase separation (LLPS)	994:1030	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
33952698	6	48	theme	liquid-liquid	994:1006	arg1	LLPS					1026:1029	LLPS	1026:1029	LLPS	1026:1029	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
33952698	8	49	theme	Gal-3	1185:1189	arg1	oligomerization/condensation					1191:1218	LLPS-like Gal-3 oligomerization/condensation	1175:1218	LLPS-like Gal-3 oligomerization/condensation	1175:1218	LLPS-like Gal-3 oligomerization/condensation was also observed on the plasma membrane and disrupted endomembranes.					
33952698	5	50	theme	fundamental	829:839	arg1	oligomerization					810:824	Gal-3 oligomerization	804:824	Gal-3 oligomerization	804:824	For mechanistic insight, we investigated the role of prolines in mediating Gal-3 oligomerization, a fundamental process required for these cell activities.					
33952698	5	50	theme	fundamental	829:839	arg1	process					841:847	a fundamental process	827:847	a fundamental process required for these cell activities	827:882	For mechanistic insight, we investigated the role of prolines in mediating Gal-3 oligomerization, a fundamental process required for these cell activities.					
33952698	7	51	dep	concentration	1135:1147	arg1	the					1131:1133	the	1131:1133	the	1131:1133	The composition of these heterooligomers is dependent on the concentration of Gal-3 as well as on the concentration and type of glycoprotein.					
33952698	9	52	theme	cell-based	1305:1314	arg1	assays					1316:1321	Molecular- and cell-based assays	1290:1321	Molecular- and cell-based assays	1290:1321	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	3	53	theme	Gal-3-mediated	454:467	arg1	functions					469:477	various Gal-3-mediated functions	446:477	various Gal-3-mediated functions	446:477	To provide some resolution to this puzzle, we individually mutated all 14 NT prolines over the first 68 residues and assessed their effects on various Gal-3-mediated functions.					
33952698	3	54	theme	NT	377:378	arg1	prolines					380:387	all 14 NT prolines	370:387	all 14 NT prolines	370:387	To provide some resolution to this puzzle, we individually mutated all 14 NT prolines over the first 68 residues and assessed their effects on various Gal-3-mediated functions.					
33952698	2	55	theme	functional	206:215	arg1	role					217:220	The functional role	202:220	The functional role of the NT with its numerous prolines	202:257	The functional role of the NT with its numerous prolines has remained enigmatic since its discovery.					
33952698	5	56	theme	cell	868:871	arg1	activities					873:882	these cell activities	862:882	these cell activities	862:882	For mechanistic insight, we investigated the role of prolines in mediating Gal-3 oligomerization, a fundamental process required for these cell activities.					
33952698	2	57	theme	numerous	241:248	arg1	prolines					250:257	its numerous prolines	237:257	its numerous prolines	237:257	The functional role of the NT with its numerous prolines has remained enigmatic since its discovery.					
33952698	6	58	gly	glycoproteins	946:958	arg1	glycoproteins					946:958	glycoproteins	946:958	glycoproteins	946:958	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
33952698	1	59	theme	long	138:141	arg1	tail					191:194	a long, aperiodic, and dynamic proline-rich N-terminal tail	136:194	a long, aperiodic, and dynamic proline-rich N-terminal tail (NT)	136:199	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	1	59	theme	long	138:141	arg1	NT					197:198	NT	197:198	NT	197:198	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	11	60	gly	glycosylated	1873:1884	arg1	receptors					1899:1907	glycosylated cell surface receptors	1873:1907	glycosylated cell surface receptors	1873:1907	Our results unveil the role of proline polymorphisms (e.g., at P64) associated with many diseases and suggest that the function of glycosylated cell surface receptors is dynamically regulated by Gal-3.					
33952698	3	61	theme	various	446:452	arg1	functions					469:477	various Gal-3-mediated functions	446:477	various Gal-3-mediated functions	446:477	To provide some resolution to this puzzle, we individually mutated all 14 NT prolines over the first 68 residues and assessed their effects on various Gal-3-mediated functions.					
33952698	6	62	theme	analogous	981:989	arg1	process					973:979	a dynamic process	963:979	a dynamic process analogous to liquid-liquid phase separation (LLPS)	963:1030	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
33952698	6	62	theme	analogous	981:989	arg1	oligomerization					906:920	Gal-3 oligomerization	900:920	Gal-3 oligomerization triggered by binding to glycoproteins	900:958	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
33952698	0	63	theme	oligomerization/phase	79:99	arg1	separation					101:110	glycan-mediated oligomerization/phase separation	63:110	glycan-mediated oligomerization/phase separation	63:110	Galectin-3 N-terminal tail prolines modulate cell activity and glycan-mediated oligomerization/phase separation.					
33952698	8	64	theme	plasma	1245:1250	arg1	membrane					1252:1259	the plasma membrane	1241:1259	the plasma membrane	1241:1259	LLPS-like Gal-3 oligomerization/condensation was also observed on the plasma membrane and disrupted endomembranes.					
33952698	9	65	dep	NT	1462:1463	arg1	F-face					1511:1516	F-face	1511:1516	F-face	1511:1516	Molecular- and cell-based assays indicate that glycan binding-triggered Gal-3 LLPS (or LLPS-like) is driven mainly by dynamic intermolecular interactions between the Gal-3 NT and the carbohydrate recognition domain (CRD) F-face, although NT-NT interactions appear to contribute to a lesser extent.					
33952698	1	66	theme	aperiodic	144:152	arg1	tail					191:194	a long, aperiodic, and dynamic proline-rich N-terminal tail	136:194	a long, aperiodic, and dynamic proline-rich N-terminal tail (NT)	136:199	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	1	66	theme	aperiodic	144:152	arg1	NT					197:198	NT	197:198	NT	197:198	Galectin-3 (Gal-3) has a long, aperiodic, and dynamic proline-rich N-terminal tail (NT).					
33952698	11	67	theme	cell	1886:1889	arg1	receptors					1899:1907	glycosylated cell surface receptors	1873:1907	glycosylated cell surface receptors	1873:1907	Our results unveil the role of proline polymorphisms (e.g., at P64) associated with many diseases and suggest that the function of glycosylated cell surface receptors is dynamically regulated by Gal-3.					
33952698	10	68	theme	NT-CRD	1651:1656	arg1	interactions					1658:1669	NT-CRD interactions	1651:1669	NT-CRD interactions	1651:1669	Mutation of each proline within the NT differentially controls NT-CRD interactions, consequently affecting glycan binding, LLPS, and cellular activities.					
33952698	0	69	theme	glycan-mediated	63:77	arg1	separation					101:110	glycan-mediated oligomerization/phase separation	63:110	glycan-mediated oligomerization/phase separation	63:110	Galectin-3 N-terminal tail prolines modulate cell activity and glycan-mediated oligomerization/phase separation.					
33952698	11	70	theme	glycosylated	1873:1884	arg1	receptors					1899:1907	glycosylated cell surface receptors	1873:1907	glycosylated cell surface receptors	1873:1907	Our results unveil the role of proline polymorphisms (e.g., at P64) associated with many diseases and suggest that the function of glycosylated cell surface receptors is dynamically regulated by Gal-3.					
33952698	2	71	theme	NT	229:230	arg1	role					217:220	The functional role	202:220	The functional role of the NT with its numerous prolines	202:257	The functional role of the NT with its numerous prolines has remained enigmatic since its discovery.					
33952698	4	72	theme	cellular	638:645	arg1	activities					647:656	Gal-3-mediated cellular activities	623:656	Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination)	623:726	Our findings show that mutation of any single proline (especially P37A, P55A, P60A, P64A/H, and P67A) dramatically and differentially inhibits Gal-3-mediated cellular activities (i.e., cell migration, activation, endocytosis, and hemagglutination).					
33952698	6	73	theme	dynamic	965:971	arg1	process					973:979	a dynamic process	963:979	a dynamic process analogous to liquid-liquid phase separation (LLPS)	963:1030	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
33952698	6	73	theme	dynamic	965:971	arg1	oligomerization					906:920	Gal-3 oligomerization	900:920	Gal-3 oligomerization triggered by binding to glycoproteins	900:958	We showed that Gal-3 oligomerization triggered by binding to glycoproteins is a dynamic process analogous to liquid-liquid phase separation (LLPS).					
32810222	6	0	theme	structural	885:894	arg1	features					896:903	several biological and structural features	862:903	several biological and structural features of 34-1-2S	862:914	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	10	1	theme	anti-HLA	1525:1532	arg1	antibodies					1534:1543	human TRALI-causing donor anti-HLA antibodies	1499:1543	human TRALI-causing donor anti-HLA antibodies	1499:1543	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	11	2	theme	superior	1634:1641	arg1	capacity					1665:1672	superior complement activation capacity	1634:1672	superior complement activation capacity	1634:1672	34-1-2S, however, displayed superior complement activation capacity, which was fully Fc dependent and not significantly dependent on Fc glycosylation.					
32810222	3	3	theme	I	504:504	arg1	mice					488:491	BALB/c mice	481:491	BALB/c mice (MHC class I haplotype H-2Kd)	481:521	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	3	3	theme	I	504:504	arg1	H-2Kd					516:520	MHC class I haplotype H-2Kd	494:520	MHC class I haplotype H-2Kd	494:520	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	6	4	theme	biological	870:879	arg1	features					896:903	several biological and structural features	862:903	several biological and structural features of 34-1-2S	862:914	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	4	5	theme	C57BL/6	567:573	arg1	H-2Kb					603:607	MHC class I haplotype H-2Kb	581:607	MHC class I haplotype H-2Kb	581:607	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	4	5	theme	C57BL/6	567:573	arg1	mice					575:578	C57BL/6 mice	567:578	C57BL/6 mice (MHC class I haplotype H-2Kb)	567:608	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	10	6	theme	human	1559:1563	arg1	antibodies					1574:1583	human anti-HLA antibodies	1559:1583	human anti-HLA antibodies from control donors	1559:1603	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	1	7	theme	deaths	214:219	arg1	cause					185:189	a leading cause	175:189	a leading cause of transfusion-related deaths	175:219	Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related deaths.					
32810222	8	8	theme	binding	1186:1192	arg1	affinity					1194:1201	binding affinity	1186:1201	binding affinity to H-2Kb	1186:1210	Regarding binding affinity to H-2Kb, only AF6-88.5.5.3 potently bound to H-2Kb, whereas 34-1-2S exhibited weak but significant cross-reactivity.					
32810222	6	9	theme	class	945:949	arg1	AF6-88.5.5.3					1017:1028	AF6-88.5.5.3	1017:1028	AF6-88.5.5.3	1017:1028	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	6	9	theme	class	945:949	arg1	antibodies					953:962	other anti-MHC class I antibodies	930:962	other anti-MHC class I antibodies	930:962	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	6	9	theme	class	945:949	arg1	SF1.1.10					1004:1011	SF1.1.10	1004:1011	SF1.1.10	1004:1011	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	4	10	theme	haplotype	593:601	arg1	H-2Kb					603:607	MHC class I haplotype H-2Kb	581:607	MHC class I haplotype H-2Kb	581:607	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	4	10	theme	haplotype	593:601	arg1	mice					575:578	C57BL/6 mice	567:578	C57BL/6 mice (MHC class I haplotype H-2Kb)	567:608	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	10	11	theme	control	1590:1596	arg1	donors					1598:1603	control donors	1590:1603	control donors	1590:1603	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	12	12	theme	complement	1947:1956	arg1	activation					1958:1967	increased Fc-mediated complement activation	1925:1967	increased Fc-mediated complement activation	1925:1967	We conclude that TRALI induction is not correlated with Fab- and Fc-binding affinities for antigen and FcγRs, respectively, nor with the composition of Fc glycans; but increased Fc-mediated complement activation is correlated with TRALI induction.					
32810222	6	13	theme	other	930:934	arg1	AF6-88.5.5.3					1017:1028	AF6-88.5.5.3	1017:1028	AF6-88.5.5.3	1017:1028	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	6	13	theme	other	930:934	arg1	antibodies					953:962	other anti-MHC class I antibodies	930:962	other anti-MHC class I antibodies	930:962	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	6	13	theme	other	930:934	arg1	SF1.1.10					1004:1011	SF1.1.10	1004:1011	SF1.1.10	1004:1011	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	2	14	theme	product	282:288	arg1	TRALI					298:302	the transfusion product trigger TRALI	266:302	the transfusion product trigger TRALI	266:302	In most cases, anti-leukocyte antibodies in the transfusion product trigger TRALI, but not all anti-leukocyte antibodies cause TRALI.					
32810222	4	15	theme	anti-MHC	634:641	arg1	AF6-88.5.5.3					660:671	anti-MHC class I antibody AF6-88.5.5.3	634:671	anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb)	634:684	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	4	15	theme	anti-MHC	634:641	arg1	anti-H-2Kb					674:683	anti-H-2Kb	674:683	anti-H-2Kb	674:683	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	5	16	theme	specific	765:772	arg1	characteristics					783:797	specific antibody characteristics	765:797	specific antibody characteristics	765:797	It remains unknown which specific antibody characteristics are responsible for eliciting TRALI.					
32810222	6	17	dep	antibodies	953:962	arg1	AF6-88.5.5.3					1017:1028	AF6-88.5.5.3	1017:1028	AF6-88.5.5.3	1017:1028	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	6	17	dep	antibodies	953:962	arg1	antibodies					953:962	other anti-MHC class I antibodies	930:962	other anti-MHC class I antibodies	930:962	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	6	17	dep	antibodies	953:962	arg1	SF1.1.10					1004:1011	SF1.1.10	1004:1011	SF1.1.10	1004:1011	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	4	18	theme	I	649:649	arg1	AF6-88.5.5.3					660:671	anti-MHC class I antibody AF6-88.5.5.3	634:671	anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb)	634:684	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	4	18	theme	I	649:649	arg1	anti-H-2Kb					674:683	anti-H-2Kb	674:683	anti-H-2Kb	674:683	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	0	19	theme	Fc-mediated	86:96	arg1	activation					109:118	increased Fc-mediated complement activation	76:118	increased Fc-mediated complement activation	76:118	Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation.					
32810222	12	20	theme	glycans	1912:1918	arg1	composition					1894:1904	the composition	1890:1904	the composition of Fc glycans	1890:1918	We conclude that TRALI induction is not correlated with Fab- and Fc-binding affinities for antigen and FcγRs, respectively, nor with the composition of Fc glycans; but increased Fc-mediated complement activation is correlated with TRALI induction.					
32810222	1	21	theme	leading	177:183	arg1	cause					185:189	a leading cause	175:189	a leading cause of transfusion-related deaths	175:219	Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related deaths.					
32810222	10	22	theme	glycosylation	1453:1465	arg1	profiles					1467:1474	Similar Fc glycosylation profiles	1442:1474	Similar Fc glycosylation profiles	1442:1474	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	0	23	theme	murine	46:51	arg1	antibody					59:66	murine TRALI antibody	46:66	murine TRALI antibody	46:66	Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation.					
32810222	3	24	theme	complex	413:419	arg1	class					427:431	the anti-major histocompatibility complex (MHC) class I	379:433	the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd)	379:463	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	10	25	theme	Similar	1442:1448	arg1	profiles					1467:1474	Similar Fc glycosylation profiles	1442:1474	Similar Fc glycosylation profiles	1442:1474	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	3	26	theme	antibody	435:442	arg1	anti-H-2Kd					453:462	anti-H-2Kd	453:462	anti-H-2Kd	453:462	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	3	26	theme	antibody	435:442	arg1	34-1-2S					444:450	the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S	379:450	the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd)	379:463	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	0	27	theme	Biological	0:9	arg1	characterization					26:41	Biological and structural characterization	0:41	Biological and structural characterization of murine TRALI antibody	0:66	Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation.					
32810222	3	28	theme	anti-major	383:392	arg1	class					427:431	the anti-major histocompatibility complex (MHC) class I	379:433	the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd)	379:463	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	0	29	theme	structural	15:24	arg1	characterization					26:41	Biological and structural characterization	0:41	Biological and structural characterization of murine TRALI antibody	0:66	Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation.					
32810222	9	30	theme	binding	1338:1344	arg1	similar					1414:1420	similar	1414:1420	similar	1414:1420	Furthermore, the binding affinity to FcγRs as well as the Fc glycan composition seemed to be similar for all antibodies.					
32810222	9	30	theme	binding	1338:1344	arg1	affinity					1346:1353	the binding affinity	1334:1353	the binding affinity to FcγRs as well as the Fc glycan composition	1334:1399	Furthermore, the binding affinity to FcγRs as well as the Fc glycan composition seemed to be similar for all antibodies.					
32810222	1	31	theme	acute	141:145	arg1	TRALI					160:164	TRALI	160:164	TRALI	160:164	Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related deaths.					
32810222	1	31	theme	acute	141:145	arg1	injury					152:157	Transfusion-related acute lung injury	121:157	Transfusion-related acute lung injury (TRALI)	121:165	Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related deaths.					
32810222	3	32	theme	class	427:431	arg1	anti-H-2Kd					453:462	anti-H-2Kd	453:462	anti-H-2Kd	453:462	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	3	32	theme	class	427:431	arg1	34-1-2S					444:450	the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S	379:450	the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd)	379:463	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	12	33	theme	Fc-binding	1822:1831	arg1	affinities					1833:1842	Fab- and Fc-binding affinities	1813:1842	Fab- and Fc-binding affinities for antigen and FcγRs, respectively	1813:1878	We conclude that TRALI induction is not correlated with Fab- and Fc-binding affinities for antigen and FcγRs, respectively, nor with the composition of Fc glycans; but increased Fc-mediated complement activation is correlated with TRALI induction.					
32810222	3	34	theme	MHC	422:424	arg1	class					427:431	the anti-major histocompatibility complex (MHC) class I	379:433	the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd)	379:463	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	10	35	theme	donor	1519:1523	arg1	antibodies					1534:1543	human TRALI-causing donor anti-HLA antibodies	1499:1543	human TRALI-causing donor anti-HLA antibodies	1499:1543	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	0	36	theme	TRALI	53:57	arg1	antibody					59:66	murine TRALI antibody	46:66	murine TRALI antibody	46:66	Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation.					
32810222	10	37	theme	human	1499:1503	arg1	antibodies					1534:1543	human TRALI-causing donor anti-HLA antibodies	1499:1543	human TRALI-causing donor anti-HLA antibodies	1499:1543	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	11	38	theme	Fc	1739:1740	arg1	glycosylation					1742:1754	Fc glycosylation	1739:1754	Fc glycosylation	1739:1754	34-1-2S, however, displayed superior complement activation capacity, which was fully Fc dependent and not significantly dependent on Fc glycosylation.					
32810222	4	39	theme	34-1-2S	731:737	arg1	dose					723:726	a high dose	716:726	a high dose of 34-1-2S	716:737	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	3	40	theme	BALB/c	481:486	arg1	mice					488:491	BALB/c mice	481:491	BALB/c mice (MHC class I haplotype H-2Kd)	481:521	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	3	40	theme	BALB/c	481:486	arg1	H-2Kd					516:520	MHC class I haplotype H-2Kd	494:520	MHC class I haplotype H-2Kd	494:520	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	3	41	dep	causes	465:470	arg1	whereas					524:530	whereas	524:530	whereas	524:530	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	3	42	theme	haplotype	506:514	arg1	mice					488:491	BALB/c mice	481:491	BALB/c mice (MHC class I haplotype H-2Kd)	481:521	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	3	42	theme	haplotype	506:514	arg1	H-2Kd					516:520	MHC class I haplotype H-2Kd	494:520	MHC class I haplotype H-2Kd	494:520	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	9	43	theme	Fc	1379:1380	arg1	composition					1389:1399	the Fc glycan composition	1375:1399	FcγRs as well as the Fc glycan composition	1358:1399	Furthermore, the binding affinity to FcγRs as well as the Fc glycan composition seemed to be similar for all antibodies.					
32810222	10	44	from	donors	1598:1603	arg1	antibodies					1574:1583	human anti-HLA antibodies	1559:1583	human anti-HLA antibodies from control donors	1559:1603	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	12	45	theme	Fab-	1813:1816	arg1	affinities					1833:1842	Fab- and Fc-binding affinities	1813:1842	Fab- and Fc-binding affinities for antigen and FcγRs, respectively	1813:1878	We conclude that TRALI induction is not correlated with Fab- and Fc-binding affinities for antigen and FcγRs, respectively, nor with the composition of Fc glycans; but increased Fc-mediated complement activation is correlated with TRALI induction.					
32810222	4	46	theme	MHC	581:583	arg1	H-2Kb					603:607	MHC class I haplotype H-2Kb	581:607	MHC class I haplotype H-2Kb	581:607	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	4	46	theme	MHC	581:583	arg1	mice					575:578	C57BL/6 mice	567:578	C57BL/6 mice (MHC class I haplotype H-2Kb)	567:608	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	7	47	theme	TRALI-causing	1084:1096	arg1	34-1-2S					1098:1104	the TRALI-causing 34-1-2S	1080:1104	the TRALI-causing 34-1-2S	1080:1104	No substantial differences were observed between the TRALI-causing 34-1-2S and the TRALI-resistant SF1.1.10 regarding binding affinity to H-2Kd.					
32810222	3	48	theme	class	498:502	arg1	mice					488:491	BALB/c mice	481:491	BALB/c mice (MHC class I haplotype H-2Kd)	481:521	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	3	48	theme	class	498:502	arg1	H-2Kd					516:520	MHC class I haplotype H-2Kd	494:520	MHC class I haplotype H-2Kd	494:520	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	11	49	dep	significantly	1712:1724	arg1	not					1708:1710	not	1708:1710	not	1708:1710	34-1-2S, however, displayed superior complement activation capacity, which was fully Fc dependent and not significantly dependent on Fc glycosylation.					
32810222	12	50	theme	TRALI	1774:1778	arg1	induction					1780:1788	TRALI induction	1774:1788	TRALI induction	1774:1788	We conclude that TRALI induction is not correlated with Fab- and Fc-binding affinities for antigen and FcγRs, respectively, nor with the composition of Fc glycans; but increased Fc-mediated complement activation is correlated with TRALI induction.					
32810222	11	51	theme	complement	1643:1652	arg1	capacity					1665:1672	superior complement activation capacity	1634:1672	superior complement activation capacity	1634:1672	34-1-2S, however, displayed superior complement activation capacity, which was fully Fc dependent and not significantly dependent on Fc glycosylation.					
32810222	6	52	theme	several	862:868	arg1	features					896:903	several biological and structural features	862:903	several biological and structural features of 34-1-2S	862:914	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	10	53	theme	anti-HLA	1565:1572	arg1	antibodies					1574:1583	human anti-HLA antibodies	1559:1583	human anti-HLA antibodies from control donors	1559:1603	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	7	54	theme	substantial	1034:1044	arg1	differences					1046:1056	No substantial differences	1031:1056	No substantial differences	1031:1056	No substantial differences were observed between the TRALI-causing 34-1-2S and the TRALI-resistant SF1.1.10 regarding binding affinity to H-2Kd.					
32810222	1	55	theme	transfusion-related	194:212	arg1	deaths					214:219	transfusion-related deaths	194:219	transfusion-related deaths	194:219	Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related deaths.					
32810222	6	56	theme	I	951:951	arg1	AF6-88.5.5.3					1017:1028	AF6-88.5.5.3	1017:1028	AF6-88.5.5.3	1017:1028	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	6	56	theme	I	951:951	arg1	antibodies					953:962	other anti-MHC class I antibodies	930:962	other anti-MHC class I antibodies	930:962	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	6	56	theme	I	951:951	arg1	SF1.1.10					1004:1011	SF1.1.10	1004:1011	SF1.1.10	1004:1011	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	2	57	theme	anti-leukocyte	317:330	arg1	antibodies					332:341	all anti-leukocyte antibodies	313:341	all anti-leukocyte antibodies	313:341	In most cases, anti-leukocyte antibodies in the transfusion product trigger TRALI, but not all anti-leukocyte antibodies cause TRALI.					
32810222	5	58	theme	antibody	774:781	arg1	characteristics					783:797	specific antibody characteristics	765:797	specific antibody characteristics	765:797	It remains unknown which specific antibody characteristics are responsible for eliciting TRALI.					
32810222	12	59	theme	TRALI	1988:1992	arg1	induction					1994:2002	TRALI induction	1988:2002	TRALI induction	1988:2002	We conclude that TRALI induction is not correlated with Fab- and Fc-binding affinities for antigen and FcγRs, respectively, nor with the composition of Fc glycans; but increased Fc-mediated complement activation is correlated with TRALI induction.					
32810222	6	60	theme	anti-MHC	936:943	arg1	AF6-88.5.5.3					1017:1028	AF6-88.5.5.3	1017:1028	AF6-88.5.5.3	1017:1028	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	6	60	theme	anti-MHC	936:943	arg1	antibodies					953:962	other anti-MHC class I antibodies	930:962	other anti-MHC class I antibodies	930:962	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	6	60	theme	anti-MHC	936:943	arg1	SF1.1.10					1004:1011	SF1.1.10	1004:1011	SF1.1.10	1004:1011	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	4	61	theme	I	591:591	arg1	H-2Kb					603:607	MHC class I haplotype H-2Kb	581:607	MHC class I haplotype H-2Kb	581:607	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	4	61	theme	I	591:591	arg1	mice					575:578	C57BL/6 mice	567:578	C57BL/6 mice (MHC class I haplotype H-2Kb)	567:608	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	12	62	theme	Fc-mediated	1935:1945	arg1	activation					1958:1967	increased Fc-mediated complement activation	1925:1967	increased Fc-mediated complement activation	1925:1967	We conclude that TRALI induction is not correlated with Fab- and Fc-binding affinities for antigen and FcγRs, respectively, nor with the composition of Fc glycans; but increased Fc-mediated complement activation is correlated with TRALI induction.					
32810222	2	63	theme	anti-leukocyte	237:250	arg1	antibodies					252:261	anti-leukocyte antibodies	237:261	anti-leukocyte antibodies in the transfusion product trigger TRALI	237:302	In most cases, anti-leukocyte antibodies in the transfusion product trigger TRALI, but not all anti-leukocyte antibodies cause TRALI.					
32810222	1	64	theme	lung	147:150	arg1	TRALI					160:164	TRALI	160:164	TRALI	160:164	Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related deaths.					
32810222	1	64	theme	lung	147:150	arg1	injury					152:157	Transfusion-related acute lung injury	121:157	Transfusion-related acute lung injury (TRALI)	121:165	Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related deaths.					
32810222	6	65	theme	34-1-2S	908:914	arg1	features					896:903	several biological and structural features	862:903	several biological and structural features of 34-1-2S	862:914	We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3.					
32810222	2	66	theme	most	225:228	arg1	cases					230:234	most cases	225:234	most cases	225:234	In most cases, anti-leukocyte antibodies in the transfusion product trigger TRALI, but not all anti-leukocyte antibodies cause TRALI.					
32810222	4	67	theme	antibody	651:658	arg1	AF6-88.5.5.3					660:671	anti-MHC class I antibody AF6-88.5.5.3	634:671	anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb)	634:684	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	4	67	theme	antibody	651:658	arg1	anti-H-2Kb					674:683	anti-H-2Kb	674:683	anti-H-2Kb	674:683	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	2	68	theme	trigger	290:296	arg1	TRALI					298:302	the transfusion product trigger TRALI	266:302	the transfusion product trigger TRALI	266:302	In most cases, anti-leukocyte antibodies in the transfusion product trigger TRALI, but not all anti-leukocyte antibodies cause TRALI.					
32810222	8	69	theme	weak	1282:1285	arg1	cross-reactivity					1303:1318	weak but significant cross-reactivity	1282:1318	weak but significant cross-reactivity	1282:1318	Regarding binding affinity to H-2Kb, only AF6-88.5.5.3 potently bound to H-2Kb, whereas 34-1-2S exhibited weak but significant cross-reactivity.					
32810222	0	70	theme	increased	76:84	arg1	activation					109:118	increased Fc-mediated complement activation	76:118	increased Fc-mediated complement activation	76:118	Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation.					
32810222	2	71	theme	transfusion	270:280	arg1	TRALI					298:302	the transfusion product trigger TRALI	266:302	the transfusion product trigger TRALI	266:302	In most cases, anti-leukocyte antibodies in the transfusion product trigger TRALI, but not all anti-leukocyte antibodies cause TRALI.					
32810222	8	72	theme	significant	1291:1301	arg1	cross-reactivity					1303:1318	weak but significant cross-reactivity	1282:1318	weak but significant cross-reactivity	1282:1318	Regarding binding affinity to H-2Kb, only AF6-88.5.5.3 potently bound to H-2Kb, whereas 34-1-2S exhibited weak but significant cross-reactivity.					
32810222	0	73	theme	complement	98:107	arg1	activation					109:118	increased Fc-mediated complement activation	76:118	increased Fc-mediated complement activation	76:118	Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation.					
32810222	4	74	theme	class	643:647	arg1	AF6-88.5.5.3					660:671	anti-MHC class I antibody AF6-88.5.5.3	634:671	anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb)	634:684	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	4	74	theme	class	643:647	arg1	anti-H-2Kb					674:683	anti-H-2Kb	674:683	anti-H-2Kb	674:683	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	12	75	theme	increased	1925:1933	arg1	activation					1958:1967	increased Fc-mediated complement activation	1925:1967	increased Fc-mediated complement activation	1925:1967	We conclude that TRALI induction is not correlated with Fab- and Fc-binding affinities for antigen and FcγRs, respectively, nor with the composition of Fc glycans; but increased Fc-mediated complement activation is correlated with TRALI induction.					
32810222	10	76	theme	Fc	1450:1451	arg1	profiles					1467:1474	Similar Fc glycosylation profiles	1442:1474	Similar Fc glycosylation profiles	1442:1474	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	4	77	theme	high	718:721	arg1	dose					723:726	a high dose	716:726	a high dose of 34-1-2S	716:737	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	12	78	theme	Fc	1909:1910	arg1	glycans					1912:1918	Fc glycans	1909:1918	Fc glycans	1909:1918	We conclude that TRALI induction is not correlated with Fab- and Fc-binding affinities for antigen and FcγRs, respectively, nor with the composition of Fc glycans; but increased Fc-mediated complement activation is correlated with TRALI induction.					
32810222	2	79	from	antibodies	252:261	arg1	TRALI					298:302	the transfusion product trigger TRALI	266:302	the transfusion product trigger TRALI	266:302	In most cases, anti-leukocyte antibodies in the transfusion product trigger TRALI, but not all anti-leukocyte antibodies cause TRALI.					
32810222	1	80	theme	Transfusion-related	121:139	arg1	TRALI					160:164	TRALI	160:164	TRALI	160:164	Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related deaths.					
32810222	1	80	theme	Transfusion-related	121:139	arg1	injury					152:157	Transfusion-related acute lung injury	121:157	Transfusion-related acute lung injury (TRALI)	121:165	Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related deaths.					
32810222	3	81	theme	histocompatibility	394:411	arg1	class					427:431	the anti-major histocompatibility complex (MHC) class I	379:433	the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd)	379:463	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	7	82	theme	TRALI-resistant	1114:1128	arg1	SF1.1.10					1130:1137	the TRALI-resistant SF1.1.10	1110:1137	the TRALI-resistant SF1.1.10 regarding binding affinity to H-2Kd	1110:1173	No substantial differences were observed between the TRALI-causing 34-1-2S and the TRALI-resistant SF1.1.10 regarding binding affinity to H-2Kd.					
32810222	0	83	theme	antibody	59:66	arg1	characterization					26:41	Biological and structural characterization	0:41	Biological and structural characterization of murine TRALI antibody	0:66	Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation.					
32810222	10	84	theme	TRALI-causing	1505:1517	arg1	antibodies					1534:1543	human TRALI-causing donor anti-HLA antibodies	1499:1543	human TRALI-causing donor anti-HLA antibodies	1499:1543	Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors.					
32810222	3	85	theme	MHC	494:496	arg1	mice					488:491	BALB/c mice	481:491	BALB/c mice (MHC class I haplotype H-2Kd)	481:521	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	3	85	theme	MHC	494:496	arg1	H-2Kd					516:520	MHC class I haplotype H-2Kd	494:520	MHC class I haplotype H-2Kd	494:520	It has been shown that the anti-major histocompatibility complex (MHC) class I antibody 34-1-2S (anti-H-2Kd) causes TRALI in BALB/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti-H-2Kd) does not.					
32810222	4	86	theme	class	585:589	arg1	H-2Kb					603:607	MHC class I haplotype H-2Kb	581:607	MHC class I haplotype H-2Kb	581:607	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	4	86	theme	class	585:589	arg1	mice					575:578	C57BL/6 mice	567:578	C57BL/6 mice (MHC class I haplotype H-2Kb)	567:608	In C57BL/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti-H-2Kb) is administered together with a high dose of 34-1-2S.					
32810222	11	87	theme	activation	1654:1663	arg1	capacity					1665:1672	superior complement activation capacity	1634:1672	superior complement activation capacity	1634:1672	34-1-2S, however, displayed superior complement activation capacity, which was fully Fc dependent and not significantly dependent on Fc glycosylation.					
32810222	7	88	theme	binding	1149:1155	arg1	affinity					1157:1164	binding affinity	1149:1164	binding affinity to H-2Kd	1149:1173	No substantial differences were observed between the TRALI-causing 34-1-2S and the TRALI-resistant SF1.1.10 regarding binding affinity to H-2Kd.					
32810222	9	89	theme	glycan	1382:1387	arg1	composition					1389:1399	the Fc glycan composition	1375:1399	FcγRs as well as the Fc glycan composition	1358:1399	Furthermore, the binding affinity to FcγRs as well as the Fc glycan composition seemed to be similar for all antibodies.					
33752337	2	0	theme	Increased	520:528	arg1	loading					530:536	Increased loading	520:536	Increased loading of the algal XRU84 into PEDOT	520:566	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
33752337	3	1	theme	storage	754:760	arg1	capacity					762:769	charge storage capacity	747:769	charge storage capacity	747:769	PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode.					
33752337	5	2	theme	rigid	1235:1239	arg1	layer					1253:1257	a rigid, dehydrated layer	1233:1257	a rigid, dehydrated layer	1233:1257	The viscoelastic properties of adsorbed protein, characterized using the ΔD/Δf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings.					
33752337	5	3	dep	rigid	1235:1239	arg1	dehydrated					1242:1251	dehydrated	1242:1251	dehydrated	1242:1251	The viscoelastic properties of adsorbed protein, characterized using the ΔD/Δf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings.					
33752337	4	4	with	microbalance	852:863	arg1	dissipation					870:880	dissipation	870:880	dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen)	870:959	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	6	5	theme	cytoskeleton	1414:1425	arg1	staining					1393:1400	fluorescent staining	1381:1400	fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h	1381:1511	Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.					
33752337	5	6	theme	protein	1143:1149	arg1	properties					1120:1129	The viscoelastic properties	1103:1129	The viscoelastic properties	1103:1129	The viscoelastic properties of adsorbed protein, characterized using the ΔD/Δf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings.					
33752337	1	7	link	algal-derived	274:286	arg1	extract					295:301	an algal-derived glycan extract	271:301	an algal-derived glycan extract	271:301	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	5	8	theme	ΔD/Δf	1176:1180	arg1	ratio					1182:1186	the ΔD/Δf ratio	1172:1186	the ΔD/Δf ratio	1172:1186	The viscoelastic properties of adsorbed protein, characterized using the ΔD/Δf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings.					
33752337	4	9	theme	protein	902:908	arg1	adsorption					910:919	protein adsorption	902:919	protein adsorption (transferrin, fibrinogen, and collagen)	902:959	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	5	10	theme	low	1273:1275	arg1	loadings					1283:1290	low XRU84 loadings	1273:1290	low XRU84 loadings	1273:1290	The viscoelastic properties of adsorbed protein, characterized using the ΔD/Δf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings.					
33752337	0	11	theme	glycan	103:108	arg1	composite					110:118	poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite	52:118	poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite	52:118	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite conducting polymer biomaterials for wound healing applications.					
33752337	2	12	theme	surface	628:634	arg1	area					636:639	a reduced surface nanoroughness and interfacial surface area	580:639	area	636:639	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
33752337	6	13	theme	Cell	1293:1296	arg1	studies					1298:1304	Cell studies	1293:1304	Cell studies using human dermal fibroblasts	1293:1335	Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.					
33752337	1	14	theme	algal-derived	274:286	arg1	extract					295:301	an algal-derived glycan extract	271:301	an algal-derived glycan extract	271:301	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	0	15	theme	-xylorhamno-uronic	84:101	arg1	composite					110:118	poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite	52:118	poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite	52:118	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite conducting polymer biomaterials for wound healing applications.					
33752337	1	16	theme	innovative	357:366	arg1	poly					218:221	the conducting polymer poly	195:221	the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract	195:301	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	16	theme	innovative	357:366	arg1	material					392:399	an innovative electrically conductive material	354:399	an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes	354:517	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	2	17	theme	interfacial	616:626	arg1	area					636:639	a reduced surface nanoroughness and interfacial surface area	580:639	area	636:639	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
33752337	6	18	theme	excellent	1350:1358	arg1	viability					1365:1373	excellent cell viability	1350:1373	excellent cell viability	1350:1373	Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.					
33752337	1	19	theme	glycan	288:293	arg1	extract					295:301	an algal-derived glycan extract	271:301	an algal-derived glycan extract	271:301	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	2	20	theme	algal	545:549	arg1	XRU84					551:555	the algal XRU84	541:555	the algal XRU84	541:555	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
33752337	3	21	theme	reduced	777:783	arg1	impedance					785:793	a reduced impedance	775:793	a reduced impedance relative to the control gold electrode	775:832	PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode.					
33752337	2	22	theme	water	665:669	arg1	angle					679:683	an increased static water contact angle	645:683	an increased static water contact angle	645:683	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
33752337	2	23	theme	contact	671:677	arg1	angle					679:683	an increased static water contact angle	645:683	an increased static water contact angle	645:683	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
33752337	6	24	theme	cell	1474:1477	arg1	adhesion					1479:1486	excellent cell adhesion	1464:1486	excellent cell adhesion	1464:1486	Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.					
33752337	4	25	theme	increased	1022:1030	arg1	loading					1038:1044	increased XRU84 loading	1022:1044	increased XRU84 loading	1022:1044	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	1	26	theme	conductive	381:390	arg1	poly					218:221	the conducting polymer poly	195:221	the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract	195:301	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	26	theme	conductive	381:390	arg1	material					392:399	an innovative electrically conductive material	354:399	an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes	354:517	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	2	27	theme	static	658:663	arg1	angle					679:683	an increased static water contact angle	645:683	an increased static water contact angle	645:683	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
33752337	1	28	theme	favorable	485:493	arg1	healing					501:507	favorable wound healing	485:507	favorable wound healing processes	485:517	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	4	29	dep	adsorption	910:919	arg1	fibrinogen					935:944	fibrinogen	935:944	fibrinogen	935:944	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	4	29	dep	adsorption	910:919	arg1	collagen					951:958	collagen	951:958	collagen	951:958	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	4	29	dep	adsorption	910:919	arg1	transferrin					922:932	transferrin	922:932	transferrin	922:932	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	4	30	theme	XRU84	1032:1036	arg1	loading					1038:1044	increased XRU84 loading	1022:1044	increased XRU84 loading	1022:1044	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	3	31	theme	relative	795:802	arg1	impedance					785:793	a reduced impedance	775:793	a reduced impedance relative to the control gold electrode	775:832	PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode.					
33752337	2	32	theme	increased	648:656	arg1	angle					679:683	an increased static water contact angle	645:683	an increased static water contact angle	645:683	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
33752337	1	33	theme	wound	495:499	arg1	healing					501:507	favorable wound healing	485:507	favorable wound healing processes	485:517	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	34	theme	conducting	199:208	arg1	PEDOT					253:257	PEDOT	253:257	PEDOT	253:257	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	34	theme	conducting	199:208	arg1	3,4-ethylenedioxythiophene					224:249	3,4-ethylenedioxythiophene	224:249	3,4-ethylenedioxythiophene	224:249	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	34	theme	conducting	199:208	arg1	glycan					334:339	Phycotrix™ [xylorhamno-uronic glycan	304:339	Phycotrix™ [xylorhamno-uronic glycan (XRU84)]	304:348	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	34	theme	conducting	199:208	arg1	poly					218:221	the conducting polymer poly	195:221	the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract	195:301	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	34	theme	conducting	199:208	arg1	material					392:399	an innovative electrically conductive material	354:399	an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes	354:517	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	0	35	theme	Fibrinogen	0:9	arg1	adsorption					38:47	Fibrinogen, collagen, and transferrin adsorption	0:47	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite	0:118	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite conducting polymer biomaterials for wound healing applications.					
33752337	4	36	theme	adsorption	910:919	arg1	study					893:897	study	893:897	study of protein adsorption (transferrin, fibrinogen, and collagen)	893:959	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	1	37	theme	healing	501:507	arg1	processes					509:517	favorable wound healing processes	485:517	favorable wound healing processes	485:517	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	3	38	theme	good	717:720	arg1	stability					733:741	good electrical stability	717:741	good electrical stability	717:741	PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode.					
33752337	1	39	theme	polymer	210:216	arg1	PEDOT					253:257	PEDOT	253:257	PEDOT	253:257	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	39	theme	polymer	210:216	arg1	3,4-ethylenedioxythiophene					224:249	3,4-ethylenedioxythiophene	224:249	3,4-ethylenedioxythiophene	224:249	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	39	theme	polymer	210:216	arg1	glycan					334:339	Phycotrix™ [xylorhamno-uronic glycan	304:339	Phycotrix™ [xylorhamno-uronic glycan (XRU84)]	304:348	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	39	theme	polymer	210:216	arg1	poly					218:221	the conducting polymer poly	195:221	the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract	195:301	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	39	theme	polymer	210:216	arg1	material					392:399	an innovative electrically conductive material	354:399	an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes	354:517	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	4	40	theme	collagen	973:980	arg1	adsorption					982:991	collagen adsorption	973:991	collagen adsorption	973:991	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	1	41	theme	processes	509:517	arg1	promotion					472:480	the promotion	468:480	the promotion of favorable wound healing processes	468:517	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	6	42	theme	excellent	1464:1472	arg1	adhesion					1479:1486	excellent cell adhesion	1464:1486	excellent cell adhesion	1464:1486	Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.					
33752337	4	43	theme	transferrin	1053:1063	arg1	adsorption					1065:1074	transferrin adsorption	1053:1074	transferrin adsorption	1053:1074	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	0	44	theme	collagen	12:19	arg1	adsorption					38:47	Fibrinogen, collagen, and transferrin adsorption	0:47	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite	0:118	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite conducting polymer biomaterials for wound healing applications.					
33752337	1	45	theme	Phycotrix™	304:313	arg1	XRU84					342:346	XRU84	342:346	XRU84	342:346	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	45	theme	Phycotrix™	304:313	arg1	poly					218:221	the conducting polymer poly	195:221	the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract	195:301	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	45	theme	Phycotrix™	304:313	arg1	glycan					334:339	Phycotrix™ [xylorhamno-uronic glycan	304:339	Phycotrix™ [xylorhamno-uronic glycan (XRU84)]	304:348	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	0	46	theme	polymer	131:137	arg1	biomaterials					139:150	polymer biomaterials	131:150	polymer biomaterials for wound healing applications	131:181	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite conducting polymer biomaterials for wound healing applications.					
33752337	1	47	theme	capable	401:407	arg1	poly					218:221	the conducting polymer poly	195:221	the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract	195:301	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	47	theme	capable	401:407	arg1	material					392:399	an innovative electrically conductive material	354:399	an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes	354:517	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	0	48	theme	transferrin	26:36	arg1	adsorption					38:47	Fibrinogen, collagen, and transferrin adsorption	0:47	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite	0:118	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite conducting polymer biomaterials for wound healing applications.					
33752337	1	49	theme	[xylorhamno-uronic	315:332	arg1	XRU84					342:346	XRU84	342:346	XRU84	342:346	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	49	theme	[xylorhamno-uronic	315:332	arg1	poly					218:221	the conducting polymer poly	195:221	the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract	195:301	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	1	49	theme	[xylorhamno-uronic	315:332	arg1	glycan					334:339	Phycotrix™ [xylorhamno-uronic glycan	304:339	Phycotrix™ [xylorhamno-uronic glycan (XRU84)]	304:348	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	0	50	theme	wound	156:160	arg1	healing					162:168	wound healing	156:168	wound healing applications	156:181	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite conducting polymer biomaterials for wound healing applications.					
33752337	6	51	theme	cell	1409:1412	arg1	cytoskeleton					1414:1425	the cell cytoskeleton	1405:1425	the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h	1405:1511	Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.					
33752337	1	52	theme	beneficial	422:431	arg1	cues					459:462	beneficial biological and electrical cues	422:462	beneficial biological and electrical cues	422:462	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	5	53	theme	viscoelastic	1107:1118	arg1	properties					1120:1129	The viscoelastic properties	1103:1129	The viscoelastic properties	1103:1129	The viscoelastic properties of adsorbed protein, characterized using the ΔD/Δf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings.					
33752337	6	54	theme	fluorescent	1381:1391	arg1	staining					1393:1400	fluorescent staining	1381:1400	fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h	1381:1511	Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.					
33752337	6	55	theme	human	1312:1316	arg1	fibroblasts					1325:1335	human dermal fibroblasts	1312:1335	human dermal fibroblasts	1312:1335	Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.					
33752337	3	56	theme	control	811:817	arg1	electrode					824:832	the control gold electrode	807:832	the control gold electrode	807:832	PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode.					
33752337	0	57	theme	healing	162:168	arg1	applications					170:181	wound healing applications	156:181	wound healing applications	156:181	Fibrinogen, collagen, and transferrin adsorption to poly(3,4-ethylenedioxythiophene)-xylorhamno-uronic glycan composite conducting polymer biomaterials for wound healing applications.					
33752337	1	58	theme	biological	433:442	arg1	cues					459:462	beneficial biological and electrical cues	422:462	beneficial biological and electrical cues	422:462	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	5	59	theme	adsorbed	1134:1141	arg1	protein					1143:1149	adsorbed protein	1134:1149	adsorbed protein	1134:1149	The viscoelastic properties of adsorbed protein, characterized using the ΔD/Δf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings.					
33752337	6	60	theme	cell	1360:1363	arg1	viability					1365:1373	excellent cell viability	1350:1373	excellent cell viability	1350:1373	Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.					
33752337	3	61	theme	gold	819:822	arg1	electrode					824:832	the control gold electrode	807:832	the control gold electrode	807:832	PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode.					
33752337	2	62	theme	surface	590:596	arg1	nanoroughness					598:610	a reduced surface nanoroughness and interfacial surface area	580:639	nanoroughness	598:610	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
33752337	3	63	theme	electrical	722:731	arg1	stability					733:741	good electrical stability	717:741	good electrical stability	717:741	PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode.					
33752337	4	64	theme	quartz	837:842	arg1	microbalance					852:863	A quartz crystal microbalance	835:863	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen)	835:959	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	6	65	theme	dermal	1318:1323	arg1	fibroblasts					1325:1335	human dermal fibroblasts	1312:1335	human dermal fibroblasts	1312:1335	Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.					
33752337	2	66	theme	reduced	582:588	arg1	nanoroughness					598:610	a reduced surface nanoroughness and interfacial surface area	580:639	nanoroughness	598:610	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
33752337	1	67	theme	electrical	448:457	arg1	cues					459:462	beneficial biological and electrical cues	422:462	beneficial biological and electrical cues	422:462	We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes.					
33752337	5	68	theme	XRU84	1277:1281	arg1	loadings					1283:1290	low XRU84 loadings	1273:1290	low XRU84 loadings	1273:1290	The viscoelastic properties of adsorbed protein, characterized using the ΔD/Δf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings.					
33752337	3	69	theme	charge	747:752	arg1	capacity					762:769	charge storage capacity	747:769	charge storage capacity	747:769	PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode.					
33752337	4	70	theme	crystal	844:850	arg1	microbalance					852:863	A quartz crystal microbalance	835:863	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen)	835:959	A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced.					
33752337	2	71	theme	XRU84	551:555	arg1	loading					530:536	Increased loading	520:536	Increased loading of the algal XRU84 into PEDOT	520:566	Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle.					
32904601	0	0	theme	Human	83:87	arg1	ACE2					89:92	Human ACE2	83:92	Human ACE2	83:92	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	1	1	theme	coronavirus	172:182	arg1	pandemic					208:215	The coronavirus disease 2019 (COVID-19) pandemic	168:215	The coronavirus disease 2019 (COVID-19) pandemic	168:215	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	4	2	gly	glycosylation	710:722	arg1	binding					759:765	the binding affinity	755:774	the binding affinity between HCoV-19 S protein and hACE2	755:810	However, glycosylation did not directly contribute to the binding affinity between HCoV-19 S protein and hACE2.					
32904601	2	3	from	glycosites	419:428	arg1	protein					451:457	HCoV-19 spike protein	437:457	the HCoV-19 spike protein (S protein)	433:469	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	2	3	from	glycosites	419:428	arg1	protein					462:468	S protein	460:468	S protein	460:468	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	1	4	theme	disease	184:190	arg1	pandemic					208:215	The coronavirus disease 2019 (COVID-19) pandemic	168:215	The coronavirus disease 2019 (COVID-19) pandemic	168:215	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	1	5	theme	biological	264:273	arg1	traits					275:280	the biological traits	260:280	the biological traits of the newly identified human coronavirus (HCoV-19) virus	260:338	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	7	6	theme	glycan	1153:1158	arg1	maps					1160:1163	The PTM and glycan maps	1141:1163	maps	1160:1163	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	0	7	theme	ACE2	89:92	arg1	Analysis					18:25	Mass Spectrometry Analysis	0:25	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2	0:92	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	2	8	theme	oligomannose	534:545	arg1	type					547:550	the oligomannose type	530:550	the oligomannose type	530:550	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	7	9	theme	hACE2	1190:1194	arg1	maps					1160:1163	The PTM and glycan maps	1141:1163	maps	1160:1163	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	2	10	theme	spectrometry	354:365	arg1	study					378:382	this mass spectrometry (MS)-based study	344:382	this mass spectrometry (MS)-based study	344:382	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	3	11	used	occupied	662:669	arg2	sites					577:581	All seven glycosylation sites	553:581	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2)	553:632	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	7	12	theme	PTM	1145:1147	arg1	maps					1160:1163	The PTM and glycan maps	1141:1163	maps	1160:1163	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	4	13	theme	HCoV-19	784:790	arg1	protein					794:800	HCoV-19 S protein	784:800	HCoV-19 S protein	784:800	However, glycosylation did not directly contribute to the binding affinity between HCoV-19 S protein and hACE2.					
32904601	6	14	theme	hACE2	1032:1036	arg1	models					1000:1005	Refined structural models	981:1005	Refined structural models of HCoV-19 S protein and hACE2	981:1036	Refined structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy structures.					
32904601	6	15	theme	Refined	981:987	arg1	models					1000:1005	Refined structural models	981:1005	Refined structural models of HCoV-19 S protein and hACE2	981:1036	Refined structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy structures.					
32904601	2	16	theme	-based	371:376	arg1	study					378:382	this mass spectrometry (MS)-based study	344:382	this mass spectrometry (MS)-based study	344:382	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	1	17	theme	2019	192:195	arg1	pandemic					208:215	The coronavirus disease 2019 (COVID-19) pandemic	168:215	The coronavirus disease 2019 (COVID-19) pandemic	168:215	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	0	18	theme	Camouflaging	102:113	arg1	Glycans					115:121	Camouflaging Glycans	102:121	Camouflaging Glycans	102:121	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	2	19	theme	spike	445:449	arg1	protein					451:457	HCoV-19 spike protein	437:457	the HCoV-19 spike protein (S protein)	433:469	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	2	19	theme	spike	445:449	arg1	protein					462:468	S protein	460:468	S protein	460:468	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	6	20	theme	published	1089:1097	arg1	structures					1129:1138	recently published cryogenic electron microscopy structures	1080:1138	recently published cryogenic electron microscopy structures	1080:1138	Refined structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy structures.					
32904601	2	21	theme	possible	410:417	arg1	glycosites					419:428	21 possible glycosites	407:428	21 possible glycosites in the HCoV-19 spike protein (S protein)	407:469	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	3	22	from	sites	577:581	arg1	enzyme					617:622	human angiotensin I converting enzyme 2	586:624	human angiotensin I converting enzyme 2 (hACE2)	586:632	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	3	22	from	sites	577:581	arg1	hACE2					627:631	hACE2	627:631	hACE2	627:631	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	7	23	theme	immune	1297:1302	arg1	response					1304:1311	the immune response	1293:1311	the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines	1293:1400	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	0	24	theme	Spectrometry	5:16	arg1	Analysis					18:25	Mass Spectrometry Analysis	0:25	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2	0:92	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	5	25	with	sites	944:948	arg1	hydroxylproline					955:969	hydroxylproline	955:969	hydroxylproline in hACE2	955:978	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	7	26	theme	HCoV-19	1168:1174	arg1	S					1176:1176	S	1176:1176	S	1176:1176	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	7	27	theme	S	1176:1176	arg1	protein					1178:1184	HCoV-19 S protein	1168:1184	HCoV-19 S protein	1168:1184	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	6	28	theme	electron	1109:1116	arg1	microscopy					1118:1127	cryogenic electron microscopy	1099:1127	recently published cryogenic electron microscopy structures	1080:1138	Refined structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy structures.					
32904601	5	29	from	sites	944:948	arg1	proteins					922:929	both proteins	917:929	both proteins	917:929	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	1	30	theme	COVID-19	198:205	arg1	pandemic					208:215	The coronavirus disease 2019 (COVID-19) pandemic	168:215	The coronavirus disease 2019 (COVID-19) pandemic	168:215	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	0	31	theme	Mass	0:3	arg1	Analysis					18:25	Mass Spectrometry Analysis	0:25	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2	0:92	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	3	32	theme	complex	682:688	arg1	N-glycans					690:698	complex N-glycans	682:698	complex N-glycans	682:698	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	4	33	theme	S	792:792	arg1	protein					794:800	HCoV-19 S protein	784:800	HCoV-19 S protein	784:800	However, glycosylation did not directly contribute to the binding affinity between HCoV-19 S protein and hACE2.					
32904601	5	34	theme	multiple	888:895	arg1	sites					908:912	multiple methylated sites	888:912	multiple methylated sites in both proteins	888:929	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	5	35	theme	Additional	813:822	arg1	PTM					857:859	PTM	857:859	PTM	857:859	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	5	35	theme	Additional	813:822	arg1	modification					843:854	Additional post-translational modification	813:854	Additional post-translational modification (PTM)	813:860	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	7	36	theme	needed	1373:1378	arg1	remedies					1380:1387	desperately needed remedies	1361:1387	desperately needed remedies	1361:1387	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	0	37	theme	Unique	127:132	arg1	Modifications					153:165	Unique Post-Translational Modifications	127:165	Unique Post-Translational Modifications	127:165	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	4	38	theme	binding	759:765	arg1	affinity					767:774	the binding affinity	755:774	the binding affinity between HCoV-19 S protein and hACE2	755:810	However, glycosylation did not directly contribute to the binding affinity between HCoV-19 S protein and hACE2.					
32904601	5	39	theme	methylated	897:906	arg1	sites					908:912	multiple methylated sites	888:912	multiple methylated sites in both proteins	888:929	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	6	40	theme	HCoV-19	1010:1016	arg1	protein					1020:1026	HCoV-19 S protein	1010:1026	HCoV-19 S protein	1010:1026	Refined structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy structures.					
32904601	6	41	theme	cryogenic	1099:1107	arg1	microscopy					1118:1127	cryogenic electron microscopy	1099:1127	recently published cryogenic electron microscopy structures	1080:1138	Refined structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy structures.					
32904601	1	42	theme	identified	295:304	arg1	virus					334:338	the newly identified human coronavirus (HCoV-19) virus	285:338	the newly identified human coronavirus (HCoV-19) virus	285:338	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	3	43	theme	I	604:604	arg1	enzyme					617:622	human angiotensin I converting enzyme 2	586:624	human angiotensin I converting enzyme 2 (hACE2)	586:632	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	3	43	theme	I	604:604	arg1	hACE2					627:631	hACE2	627:631	hACE2	627:631	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	2	44	theme	mass	349:352	arg1	spectrometry					354:365	mass spectrometry	349:365	this mass spectrometry (MS)-based study	344:382	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	2	44	theme	mass	349:352	arg1	MS					368:369	MS	368:369	MS	368:369	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	7	45	theme	host	1273:1276	arg1	attachment					1278:1287	host attachment	1273:1287	host attachment	1273:1287	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	0	46	theme	Emerging	36:43	arg1	Protein					71:77	Newly Emerging Coronavirus HCoV-19 Spike Protein	30:77	Newly Emerging Coronavirus HCoV-19 Spike Protein	30:77	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	1	47	theme	human	306:310	arg1	virus					334:338	the newly identified human coronavirus (HCoV-19) virus	285:338	the newly identified human coronavirus (HCoV-19) virus	285:338	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	3	48	theme	converting	606:615	arg1	enzyme					617:622	human angiotensin I converting enzyme 2	586:624	human angiotensin I converting enzyme 2 (hACE2)	586:632	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	3	48	theme	converting	606:615	arg1	hACE2					627:631	hACE2	627:631	hACE2	627:631	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	5	49	with	sites	908:912	arg1	hydroxylproline					955:969	hydroxylproline	955:969	hydroxylproline in hACE2	955:978	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	6	50	theme	S	1018:1018	arg1	protein					1020:1026	HCoV-19 S protein	1010:1026	HCoV-19 S protein	1010:1026	Refined structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy structures.					
32904601	2	51	theme	HCoV-19	437:443	arg1	protein					451:457	HCoV-19 spike protein	437:457	the HCoV-19 spike protein (S protein)	433:469	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	2	51	theme	HCoV-19	437:443	arg1	protein					462:468	S protein	460:468	S protein	460:468	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	1	52	theme	coronavirus	312:322	arg1	virus					334:338	the newly identified human coronavirus (HCoV-19) virus	285:338	the newly identified human coronavirus (HCoV-19) virus	285:338	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	0	53	theme	Post-Translational	134:151	arg1	Modifications					153:165	Unique Post-Translational Modifications	127:165	Unique Post-Translational Modifications	127:165	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	3	54	theme	human	586:590	arg1	enzyme					617:622	human angiotensin I converting enzyme 2	586:624	human angiotensin I converting enzyme 2 (hACE2)	586:632	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	3	54	theme	human	586:590	arg1	hACE2					627:631	hACE2	627:631	hACE2	627:631	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	7	55	theme	knowledge	1336:1344	arg1	response					1304:1311	the immune response	1293:1311	the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines	1293:1400	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	7	55	theme	knowledge	1336:1344	arg1	attachment					1278:1287	host attachment	1273:1287	host attachment	1273:1287	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	6	56	theme	protein	1020:1026	arg1	models					1000:1005	Refined structural models	981:1005	Refined structural models of HCoV-19 S protein and hACE2	981:1036	Refined structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy structures.					
32904601	0	57	theme	HCoV-19	57:63	arg1	Protein					71:77	Newly Emerging Coronavirus HCoV-19 Spike Protein	30:77	Newly Emerging Coronavirus HCoV-19 Spike Protein	30:77	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	2	58	theme	S	460:460	arg1	protein					451:457	HCoV-19 spike protein	437:457	the HCoV-19 spike protein (S protein)	433:469	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	2	58	theme	S	460:460	arg1	protein					462:468	S protein	460:468	S protein	460:468	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	0	59	theme	Coronavirus	45:55	arg1	Protein					71:77	Newly Emerging Coronavirus HCoV-19 Spike Protein	30:77	Newly Emerging Coronavirus HCoV-19 Spike Protein	30:77	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	1	60	theme	HCoV-19	325:331	arg1	virus					334:338	the newly identified human coronavirus (HCoV-19) virus	285:338	the newly identified human coronavirus (HCoV-19) virus	285:338	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	3	61	theme	angiotensin	592:602	arg1	enzyme					617:622	human angiotensin I converting enzyme 2	586:624	human angiotensin I converting enzyme 2 (hACE2)	586:632	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	3	61	theme	angiotensin	592:602	arg1	hACE2					627:631	hACE2	627:631	hACE2	627:631	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	3	62	gly	glycosylation	563:575	arg2	sites					577:581	All seven glycosylation sites	553:581	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2)	553:632	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	3	62	gly	glycosylation	563:575	arg2	seven					557:561	seven	557:561	seven	557:561	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	7	63	theme	HCoV-19	1316:1322	arg1	response					1304:1311	the immune response	1293:1311	the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines	1293:1400	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	7	63	theme	HCoV-19	1316:1322	arg1	attachment					1278:1287	host attachment	1273:1287	host attachment	1273:1287	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	5	64	theme	multiple	935:942	arg1	sites					944:948	multiple sites	935:948	multiple sites with hydroxylproline in hACE2	935:978	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	7	65	theme	structural	1215:1224	arg1	details					1226:1232	additional structural details	1204:1232	additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines	1204:1400	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	5	66	from	sites	908:912	arg1	proteins					922:929	both proteins	917:929	both proteins	917:929	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	1	67	theme	worldwide	228:236	arg1	efforts					238:244	worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus	228:338	worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus	228:338	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	6	68	theme	microscopy	1118:1127	arg1	structures					1129:1138	recently published cryogenic electron microscopy structures	1080:1138	recently published cryogenic electron microscopy structures	1080:1138	Refined structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy structures.					
32904601	2	69	gly	glycosites	419:428	arg2	glycosites					419:428	21 possible glycosites	407:428	21 possible glycosites in the HCoV-19 spike protein (S protein)	407:469	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	7	70	theme	additional	1204:1213	arg1	details					1226:1232	additional structural details	1204:1232	additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines	1204:1400	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	0	71	theme	Protein	71:77	arg1	Analysis					18:25	Mass Spectrometry Analysis	0:25	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2	0:92	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	6	72	theme	structural	989:998	arg1	models					1000:1005	Refined structural models	981:1005	Refined structural models of HCoV-19 S protein and hACE2	981:1036	Refined structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy structures.					
32904601	5	73	theme	post-translational	824:841	arg1	PTM					857:859	PTM	857:859	PTM	857:859	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	5	73	theme	post-translational	824:841	arg1	modification					843:854	Additional post-translational modification	813:854	Additional post-translational modification (PTM)	813:860	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
32904601	7	74	theme	protein	1178:1184	arg1	maps					1160:1163	The PTM and glycan maps	1141:1163	maps	1160:1163	The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines.					
32904601	2	75	used	occupied	490:497	arg2	20					472:473	20	472:473	20	472:473	In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by N-glycans, predominantly of the oligomannose type.					
32904601	0	76	theme	Spike	65:69	arg1	Protein					71:77	Newly Emerging Coronavirus HCoV-19 Spike Protein	30:77	Newly Emerging Coronavirus HCoV-19 Spike Protein	30:77	Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications.					
32904601	1	77	theme	virus	334:338	arg1	traits					275:280	the biological traits	260:280	the biological traits of the newly identified human coronavirus (HCoV-19) virus	260:338	The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus.					
32904601	3	78	theme	glycosylation	563:575	arg1	sites					577:581	All seven glycosylation sites	553:581	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2)	553:632	All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex N-glycans.					
32904601	5	79	from	hydroxylproline	955:969	arg1	hACE2					974:978	hACE2	974:978	hACE2	974:978	Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2.					
33792699	7	0	theme	bisecting-GlcNAc	1374:1389	arg1	presence					1360:1367	the presence	1356:1367	the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin	1356:1426	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	6	1	theme	stronger	1017:1024	arg1	binding					1026:1032	significantly stronger binding	1003:1032	significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells	1003:1191	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	7	2	theme	chromatography/mass	1250:1268	arg1	spectrometry					1270:1281	liquid chromatography/mass spectrometry	1243:1281	liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells	1243:1344	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	3	3	theme	mesothelial	550:560	arg1	cells					562:566	normal mesothelial cells	543:566	normal mesothelial cells	543:566	ERC/mesothelin is a glycoprotein that is highly expressed on several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells.					
33792699	4	4	theme	approved	623:630	arg1	ERC/mesothelin					663:676	no clinically approved therapeutic antibody targeting ERC/mesothelin	609:676	no clinically approved therapeutic antibody targeting ERC/mesothelin	609:676	This is a predicted reason why there is no clinically approved therapeutic antibody targeting ERC/mesothelin.					
33792699	0	5	theme	mesothelioma	88:99	arg1	cells					101:105	epithelioid mesothelioma cells	76:105	epithelioid mesothelioma cells	76:105	Bisecting-GlcNAc on Asn388 is characteristic to ERC/mesothelin expressed on epithelioid mesothelioma cells.					
33792699	7	6	theme	spectrometry	1270:1281	arg1	analysis					1283:1290	liquid chromatography/mass spectrometry analysis	1243:1290	liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells	1243:1344	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	6	7	theme	mesothelial	1215:1225	arg1	cells					1227:1231	normal mesothelial cells	1208:1231	normal mesothelial cells	1208:1231	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	6	8	theme	so-called	1101:1109	arg1	structure					1128:1136	a so-called bisecting-GlcNAc structure	1099:1136	a so-called bisecting-GlcNAc structure	1099:1136	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	0	9	from	Bisecting-GlcNAc	0:15	arg1	Asn388					20:25	Asn388	20:25	Asn388	20:25	Bisecting-GlcNAc on Asn388 is characteristic to ERC/mesothelin expressed on epithelioid mesothelioma cells.					
33792699	4	10	theme	antibody	644:651	arg1	ERC/mesothelin					663:676	no clinically approved therapeutic antibody targeting ERC/mesothelin	609:676	no clinically approved therapeutic antibody targeting ERC/mesothelin	609:676	This is a predicted reason why there is no clinically approved therapeutic antibody targeting ERC/mesothelin.					
33792699	8	11	theme	selective	1540:1548	arg1	antibody					1571:1578	a highly selective and safe therapeutic antibody	1531:1578	a highly selective and safe therapeutic antibody for epithelioid mesothelioma	1531:1607	These results suggest that this glycoproteome could serve as a potential target for the generation of a highly selective and safe therapeutic antibody for epithelioid mesothelioma.					
33792699	7	12	theme	liquid	1243:1248	arg1	spectrometry					1270:1281	liquid chromatography/mass spectrometry	1243:1281	liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells	1243:1344	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	4	13	theme	therapeutic	632:642	arg1	ERC/mesothelin					663:676	no clinically approved therapeutic antibody targeting ERC/mesothelin	609:676	no clinically approved therapeutic antibody targeting ERC/mesothelin	609:676	This is a predicted reason why there is no clinically approved therapeutic antibody targeting ERC/mesothelin.					
33792699	5	14	theme	distinct	865:872	arg1	feature					874:880	a distinct feature	863:880	a distinct feature of epithelioid mesothelioma cells	863:914	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	6	15	theme	patient	978:984	arg1	specimens					986:994	patient specimens	978:994	patient specimens	978:994	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	6	16	theme	normal	1208:1213	arg1	cells					1227:1231	normal mesothelial cells	1208:1231	normal mesothelial cells	1208:1231	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	2	17	theme	patients	320:327	arg1	poor					332:335	poor	332:335	poor	332:335	The prognosis of mesothelioma patients is poor and there is no effective molecular-targeting therapy as yet.					
33792699	2	17	theme	patients	320:327	arg1	prognosis					294:302	The prognosis	290:302	The prognosis of mesothelioma patients	290:327	The prognosis of mesothelioma patients is poor and there is no effective molecular-targeting therapy as yet.					
33792699	3	18	theme	cancers	477:483	arg1	types					468:472	several types	460:472	several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells	460:566	ERC/mesothelin is a glycoprotein that is highly expressed on several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells.					
33792699	5	19	theme	normal	818:823	arg1	cells					837:841	normal mesothelial cells	818:841	normal mesothelial cells	818:841	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	7	20	from	cells	1340:1344	arg1	ERC/mesothelin					1295:1308	ERC/mesothelin	1295:1308	ERC/mesothelin from epithelioid mesothelioma cells	1295:1344	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	5	21	theme	present	686:692	arg1	study					694:698	the present study	682:698	the present study	682:698	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	2	22	theme	mesothelioma	307:318	arg1	patients					320:327	mesothelioma patients	307:327	mesothelioma patients	307:327	The prognosis of mesothelioma patients is poor and there is no effective molecular-targeting therapy as yet.					
33792699	3	23	gly	glycoprotein	419:430	arg1	ERC/mesothelin					399:412	ERC/mesothelin	399:412	ERC/mesothelin	399:412	ERC/mesothelin is a glycoprotein that is highly expressed on several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells.					
33792699	3	23	gly	glycoprotein	419:430	arg1	glycoprotein					419:430	a glycoprotein	417:430	a glycoprotein that is highly expressed on several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells	417:566	ERC/mesothelin is a glycoprotein that is highly expressed on several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells.					
33792699	5	24	theme	mesothelial	825:835	arg1	cells					837:841	normal mesothelial cells	818:841	normal mesothelial cells	818:841	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	6	25	contain	having	1092:1097	arg2	structure					1128:1136	a so-called bisecting-GlcNAc structure	1099:1136	a so-called bisecting-GlcNAc structure	1099:1136	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	6	25	contain	having	1092:1097	arg1	N-glycans					1082:1090	complex-type N-glycans	1069:1090	complex-type N-glycans having a so-called bisecting-GlcNAc structure	1069:1136	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	6	26	from	cells	1187:1191	arg1	ERC/mesothelin					1142:1155	ERC/mesothelin	1142:1155	ERC/mesothelin from epithelioid mesothelioma cells	1142:1191	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	6	27	theme	bisecting-GlcNAc	1111:1126	arg1	structure					1128:1136	a so-called bisecting-GlcNAc structure	1099:1136	a so-called bisecting-GlcNAc structure	1099:1136	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	5	28	theme	epithelioid	885:895	arg1	cells					910:914	epithelioid mesothelioma cells	885:914	epithelioid mesothelioma cells	885:914	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	6	29	theme	complex-type	1069:1080	arg1	N-glycans					1082:1090	complex-type N-glycans	1069:1090	complex-type N-glycans having a so-called bisecting-GlcNAc structure	1069:1136	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	6	30	theme	epithelioid	1162:1172	arg1	cells					1187:1191	epithelioid mesothelioma cells	1162:1191	epithelioid mesothelioma cells	1162:1191	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	5	31	theme	cells	910:914	arg1	feature					874:880	a distinct feature	863:880	a distinct feature of epithelioid mesothelioma cells	863:914	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	5	32	theme	mesothelioma	897:908	arg1	cells					910:914	epithelioid mesothelioma cells	885:914	epithelioid mesothelioma cells	885:914	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	7	33	theme	ERC/mesothelin	1413:1426	arg1	Asn388					1403:1408	Asn388	1403:1408	Asn388 of ERC/mesothelin	1403:1426	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	6	34	theme	mesothelioma	1174:1185	arg1	cells					1187:1191	epithelioid mesothelioma cells	1162:1191	epithelioid mesothelioma cells	1162:1191	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	3	35	theme	normal	543:548	arg1	cells					562:566	normal mesothelial cells	543:566	normal mesothelial cells	543:566	ERC/mesothelin is a glycoprotein that is highly expressed on several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells.					
33792699	5	36	theme	epithelioid	781:791	arg1	mesothelioma					793:804	epithelioid mesothelioma	781:804	epithelioid mesothelioma	781:804	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	6	37	theme	ERC/mesothelin	947:960	arg1	analysis					935:942	Lectin microarray analysis	917:942	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens	917:994	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	8	38	theme	antibody	1571:1578	arg1	generation					1517:1526	the generation	1513:1526	the generation of a highly selective and safe therapeutic antibody for epithelioid mesothelioma	1513:1607	These results suggest that this glycoproteome could serve as a potential target for the generation of a highly selective and safe therapeutic antibody for epithelioid mesothelioma.					
33792699	3	39	theme	epithelioid	495:505	arg1	mesothelioma					507:518	epithelioid mesothelioma	495:518	epithelioid mesothelioma	495:518	ERC/mesothelin is a glycoprotein that is highly expressed on several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells.					
33792699	3	40	theme	several	460:466	arg1	types					468:472	several types	460:472	several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells	460:566	ERC/mesothelin is a glycoprotein that is highly expressed on several types of cancers including epithelioid mesothelioma, but also expressed on normal mesothelial cells.					
33792699	7	41	theme	epithelioid	1315:1325	arg1	cells					1340:1344	epithelioid mesothelioma cells	1315:1344	epithelioid mesothelioma cells	1315:1344	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	1	42	theme	epithelioid-type	236:251	arg1	types					229:233	three types	223:233	three types: epithelioid-type, sarcomatoid-type and biphasic-type	223:287	Mesothelioma is a highly aggressive tumour associated with asbestos exposure and is histologically classified into three types: epithelioid-type, sarcomatoid-type and biphasic-type.					
33792699	6	43	theme	lectin	1044:1049	arg1	binding					1026:1032	significantly stronger binding	1003:1032	significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells	1003:1191	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	5	44	theme	differential	720:731	arg1	glycosylation					733:745	the differential glycosylation	716:745	the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells	716:841	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	8	45	theme	epithelioid	1584:1594	arg1	mesothelioma					1596:1607	epithelioid mesothelioma	1584:1607	epithelioid mesothelioma	1584:1607	These results suggest that this glycoproteome could serve as a potential target for the generation of a highly selective and safe therapeutic antibody for epithelioid mesothelioma.					
33792699	4	46	theme	targeting	653:661	arg1	ERC/mesothelin					663:676	no clinically approved therapeutic antibody targeting ERC/mesothelin	609:676	no clinically approved therapeutic antibody targeting ERC/mesothelin	609:676	This is a predicted reason why there is no clinically approved therapeutic antibody targeting ERC/mesothelin.					
33792699	6	47	theme	PHA-E4	1037:1042	arg1	lectin					1044:1049	PHA-E4 lectin	1037:1049	PHA-E4 lectin	1037:1049	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	6	48	theme	microarray	924:933	arg1	analysis					935:942	Lectin microarray analysis	917:942	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens	917:994	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	1	49	theme	aggressive	133:142	arg1	tumour					144:149	a highly aggressive tumour	124:149	a highly aggressive tumour associated with asbestos exposure	124:183	Mesothelioma is a highly aggressive tumour associated with asbestos exposure and is histologically classified into three types: epithelioid-type, sarcomatoid-type and biphasic-type.					
33792699	1	49	theme	aggressive	133:142	arg1	Mesothelioma					108:119	Mesothelioma	108:119	Mesothelioma	108:119	Mesothelioma is a highly aggressive tumour associated with asbestos exposure and is histologically classified into three types: epithelioid-type, sarcomatoid-type and biphasic-type.					
33792699	4	50	theme	predicted	579:587	arg1	reason					589:594	a predicted reason	577:594	a predicted reason why there is no clinically approved therapeutic antibody targeting ERC/mesothelin	577:676	This is a predicted reason why there is no clinically approved therapeutic antibody targeting ERC/mesothelin.					
33792699	4	50	theme	predicted	579:587	arg1	This					569:572	This	569:572	This	569:572	This is a predicted reason why there is no clinically approved therapeutic antibody targeting ERC/mesothelin.					
33792699	6	51	theme	Lectin	917:922	arg1	analysis					935:942	Lectin microarray analysis	917:942	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens	917:994	Lectin microarray analysis of ERC/mesothelin using cells and patient specimens showed significantly stronger binding of PHA-E4 lectin, which recognizes complex-type N-glycans having a so-called bisecting-GlcNAc structure, to ERC/mesothelin from epithelioid mesothelioma cells than that from normal mesothelial cells.					
33792699	8	52	theme	therapeutic	1559:1569	arg1	antibody					1571:1578	a highly selective and safe therapeutic antibody	1531:1578	a highly selective and safe therapeutic antibody for epithelioid mesothelioma	1531:1607	These results suggest that this glycoproteome could serve as a potential target for the generation of a highly selective and safe therapeutic antibody for epithelioid mesothelioma.					
33792699	2	53	theme	molecular-targeting	363:381	arg1	therapy					383:389	no effective molecular-targeting therapy	350:389	no effective molecular-targeting therapy	350:389	The prognosis of mesothelioma patients is poor and there is no effective molecular-targeting therapy as yet.					
33792699	8	54	theme	safe	1554:1557	arg1	antibody					1571:1578	a highly selective and safe therapeutic antibody	1531:1578	a highly selective and safe therapeutic antibody for epithelioid mesothelioma	1531:1607	These results suggest that this glycoproteome could serve as a potential target for the generation of a highly selective and safe therapeutic antibody for epithelioid mesothelioma.					
33792699	5	55	theme	ERC/mesothelin	755:768	arg1	present					770:776	ERC/mesothelin present	755:776	ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells	755:841	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	2	56	theme	effective	353:361	arg1	therapy					383:389	no effective molecular-targeting therapy	350:389	no effective molecular-targeting therapy	350:389	The prognosis of mesothelioma patients is poor and there is no effective molecular-targeting therapy as yet.					
33792699	1	57	theme	sarcomatoid-type	254:269	arg1	types					229:233	three types	223:233	three types: epithelioid-type, sarcomatoid-type and biphasic-type	223:287	Mesothelioma is a highly aggressive tumour associated with asbestos exposure and is histologically classified into three types: epithelioid-type, sarcomatoid-type and biphasic-type.					
33792699	0	58	theme	epithelioid	76:86	arg1	cells					101:105	epithelioid mesothelioma cells	76:105	epithelioid mesothelioma cells	76:105	Bisecting-GlcNAc on Asn388 is characteristic to ERC/mesothelin expressed on epithelioid mesothelioma cells.					
33792699	8	59	theme	potential	1492:1500	arg1	glycoproteome					1461:1473	this glycoproteome	1456:1473	this glycoproteome	1456:1473	These results suggest that this glycoproteome could serve as a potential target for the generation of a highly selective and safe therapeutic antibody for epithelioid mesothelioma.					
33792699	8	59	theme	potential	1492:1500	arg1	target					1502:1507	a potential target	1490:1507	a potential target for the generation of a highly selective and safe therapeutic antibody for epithelioid mesothelioma	1490:1607	These results suggest that this glycoproteome could serve as a potential target for the generation of a highly selective and safe therapeutic antibody for epithelioid mesothelioma.					
33792699	5	60	from	present	770:776	arg1	cells					837:841	normal mesothelial cells	818:841	normal mesothelial cells	818:841	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	5	60	from	present	770:776	arg1	that					810:813	that	810:813	that	810:813	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	5	60	from	present	770:776	arg1	mesothelioma					793:804	epithelioid mesothelioma	781:804	epithelioid mesothelioma	781:804	In the present study, we focussed on the differential glycosylation between ERC/mesothelin present on epithelioid mesothelioma and that on normal mesothelial cells and aimed to reveal a distinct feature of epithelioid mesothelioma cells.					
33792699	7	61	from	analysis	1283:1290	arg1	ERC/mesothelin					1295:1308	ERC/mesothelin	1295:1308	ERC/mesothelin from epithelioid mesothelioma cells	1295:1344	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	7	62	theme	mesothelioma	1327:1338	arg1	cells					1340:1344	epithelioid mesothelioma cells	1315:1344	epithelioid mesothelioma cells	1315:1344	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	7	63	attach	attached	1391:1398	arg2	bisecting-GlcNAc					1374:1389	a bisecting-GlcNAc	1372:1389	a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin	1372:1426	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	7	63	attach	attached	1391:1398	arg1	Asn388					1403:1408	Asn388	1403:1408	Asn388 of ERC/mesothelin	1403:1426	Further, liquid chromatography/mass spectrometry analysis on ERC/mesothelin from epithelioid mesothelioma cells confirmed the presence of a bisecting-GlcNAc attached to Asn388 of ERC/mesothelin.					
33792699	1	64	theme	asbestos	167:174	arg1	exposure					176:183	asbestos exposure	167:183	asbestos exposure	167:183	Mesothelioma is a highly aggressive tumour associated with asbestos exposure and is histologically classified into three types: epithelioid-type, sarcomatoid-type and biphasic-type.					
33792699	1	65	theme	biphasic-type	275:287	arg1	types					229:233	three types	223:233	three types: epithelioid-type, sarcomatoid-type and biphasic-type	223:287	Mesothelioma is a highly aggressive tumour associated with asbestos exposure and is histologically classified into three types: epithelioid-type, sarcomatoid-type and biphasic-type.					
32240740	8	0	theme	notoginseng	1146:1156	arg1	application					1125:1135	the application	1121:1135	the application of Panax notoginseng	1121:1156	These results will provide basis for further investigation of structure-activity relationships of Panax notoginseng polysaccharides and be useful for the application of Panax notoginseng.					
32240740	1	1	theme	pharmacological	168:182	arg1	effects					184:190	extensive pharmacological effects	158:190	extensive pharmacological effects	158:190	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	7	2	theme	rhamnogalacturonan	932:949	arg1	domains					962:968	rhamnogalacturonan II (RG-II) domains	932:968	rhamnogalacturonan II (RG-II) domains	932:968	PNPA-1B, PNPA-2B and PNPA-3B consisted of homogalacturonan (HG) as major domains, together with different ratios of RG-I and rhamnogalacturonan II (RG-II) domains.					
32240740	8	3	theme	polysaccharides	1087:1101	arg1	relationships					1052:1064	structure-activity relationships	1033:1064	structure-activity relationships of Panax notoginseng polysaccharides	1033:1101	These results will provide basis for further investigation of structure-activity relationships of Panax notoginseng polysaccharides and be useful for the application of Panax notoginseng.					
32240740	7	4	theme	major	874:878	arg1	domains					880:886	major domains	874:886	major domains	874:886	PNPA-1B, PNPA-2B and PNPA-3B consisted of homogalacturonan (HG) as major domains, together with different ratios of RG-I and rhamnogalacturonan II (RG-II) domains.					
32240740	8	5	theme	further	1008:1014	arg1	investigation					1016:1028	further investigation	1008:1028	further investigation of structure-activity relationships of Panax notoginseng polysaccharides	1008:1101	These results will provide basis for further investigation of structure-activity relationships of Panax notoginseng polysaccharides and be useful for the application of Panax notoginseng.					
32240740	7	6	theme	RG-I	923:926	arg1	ratios					913:918	different ratios	903:918	different ratios of RG-I	903:926	PNPA-1B, PNPA-2B and PNPA-3B consisted of homogalacturonan (HG) as major domains, together with different ratios of RG-I and rhamnogalacturonan II (RG-II) domains.					
32240740	6	7	with	pectin	738:743	arg1	chains					799:804	1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	750:804	1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	750:804	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	8	8	theme	structure-activity	1033:1050	arg1	relationships					1052:1064	structure-activity relationships	1033:1064	structure-activity relationships of Panax notoginseng polysaccharides	1033:1101	These results will provide basis for further investigation of structure-activity relationships of Panax notoginseng polysaccharides and be useful for the application of Panax notoginseng.					
32240740	2	9	from	notoginseng	248:258	arg1	polysaccharides					221:235	water-soluble polysaccharides	207:235	water-soluble polysaccharides from Panax notoginseng	207:258	In this work, water-soluble polysaccharides from Panax notoginseng were isolated and fractionated.					
32240740	4	10	theme	magnetic	486:493	arg1	resonance					495:503	nuclear magnetic resonance	478:503	nuclear magnetic resonance	478:503	Monosaccharide composition, enzymatic hydrolysis, nuclear magnetic resonance and methylation analysis were combined to characterize their structures.					
32240740	1	11	theme	Panax	86:90	arg1	medicine					141:148	a widely used traditional Chinese medicine	107:148	a widely used traditional Chinese medicine	107:148	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	1	11	theme	Panax	86:90	arg1	notoginseng					92:102	Panax notoginseng	86:102	Panax notoginseng	86:102	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	4	12	theme	methylation	509:519	arg1	analysis					521:528	methylation analysis	509:528	methylation analysis	509:528	Monosaccharide composition, enzymatic hydrolysis, nuclear magnetic resonance and methylation analysis were combined to characterize their structures.					
32240740	1	13	contain	has	154:156	arg2	effects					184:190	extensive pharmacological effects	158:190	extensive pharmacological effects	158:190	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	1	13	contain	has	154:156	arg1	medicine					141:148	a widely used traditional Chinese medicine	107:148	a widely used traditional Chinese medicine	107:148	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	1	13	contain	has	154:156	arg1	notoginseng					92:102	Panax notoginseng	86:102	Panax notoginseng	86:102	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	8	14	theme	relationships	1052:1064	arg1	investigation					1016:1028	further investigation	1008:1028	further investigation of structure-activity relationships of Panax notoginseng polysaccharides	1008:1101	These results will provide basis for further investigation of structure-activity relationships of Panax notoginseng polysaccharides and be useful for the application of Panax notoginseng.					
32240740	6	15	theme	type	733:736	arg1	pectin					738:743	a typical rhamnogalacturonan I (RG-I) type pectin	695:743	a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	695:804	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	6	15	theme	type	733:736	arg1	PNPA-3A					683:689	PNPA-3A	683:689	PNPA-3A	683:689	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	0	16	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of water-soluble polysaccharides	0:51	Structural analysis of water-soluble polysaccharides isolated from Panax notoginseng.					
32240740	8	17	theme	notoginseng	1075:1085	arg1	polysaccharides					1087:1101	Panax notoginseng polysaccharides	1069:1101	Panax notoginseng polysaccharides	1069:1101	These results will provide basis for further investigation of structure-activity relationships of Panax notoginseng polysaccharides and be useful for the application of Panax notoginseng.					
32240740	0	18	theme	water-soluble	23:35	arg1	polysaccharides					37:51	water-soluble polysaccharides	23:51	water-soluble polysaccharides	23:51	Structural analysis of water-soluble polysaccharides isolated from Panax notoginseng.					
32240740	3	19	dep	fractions	345:353	arg1	PNPA-3A					392:398	PNPA-3A	392:398	PNPA-3A	392:398	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	19	dep	fractions	345:353	arg1	PNPA-1B					365:371	PNPA-1B	365:371	PNPA-1B	365:371	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	19	dep	fractions	345:353	arg1	PNPA-2A					374:380	PNPA-2A	374:380	PNPA-2A	374:380	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	19	dep	fractions	345:353	arg1	PNPA-3B					404:410	PNPA-3B	404:410	PNPA-3B	404:410	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	19	dep	fractions	345:353	arg1	fractions					345:353	six pectin fractions	334:353	six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B)	334:411	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	19	dep	fractions	345:353	arg1	PNPA-1A					356:362	PNPA-1A	356:362	PNPA-1A	356:362	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	19	dep	fractions	345:353	arg1	PNPA-2B					383:389	PNPA-2B	383:389	PNPA-2B	383:389	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	8	20	theme	Panax	1069:1073	arg1	polysaccharides					1087:1101	Panax notoginseng polysaccharides	1069:1101	Panax notoginseng polysaccharides	1069:1101	These results will provide basis for further investigation of structure-activity relationships of Panax notoginseng polysaccharides and be useful for the application of Panax notoginseng.					
32240740	6	21	theme	RG-I	727:730	arg1	pectin					738:743	a typical rhamnogalacturonan I (RG-I) type pectin	695:743	a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	695:804	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	6	21	theme	RG-I	727:730	arg1	PNPA-3A					683:689	PNPA-3A	683:689	PNPA-3A	683:689	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	8	22	theme	Panax	1140:1144	arg1	notoginseng					1146:1156	Panax notoginseng	1140:1156	Panax notoginseng	1140:1156	These results will provide basis for further investigation of structure-activity relationships of Panax notoginseng polysaccharides and be useful for the application of Panax notoginseng.					
32240740	1	23	theme	used	116:119	arg1	medicine					141:148	a widely used traditional Chinese medicine	107:148	a widely used traditional Chinese medicine	107:148	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	1	23	theme	used	116:119	arg1	notoginseng					92:102	Panax notoginseng	86:102	Panax notoginseng	86:102	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	3	24	theme	starch-like	296:306	arg1	PNPN					324:327	PNPN	324:327	PNPN	324:327	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	24	theme	starch-like	296:306	arg1	polysaccharide					308:321	One starch-like polysaccharide	292:321	One starch-like polysaccharide (PNPN)	292:328	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	4	25	theme	enzymatic	456:464	arg1	hydrolysis					466:475	enzymatic hydrolysis	456:475	enzymatic hydrolysis	456:475	Monosaccharide composition, enzymatic hydrolysis, nuclear magnetic resonance and methylation analysis were combined to characterize their structures.					
32240740	6	26	theme	I	724:724	arg1	pectin					738:743	a typical rhamnogalacturonan I (RG-I) type pectin	695:743	a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	695:804	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	6	26	theme	I	724:724	arg1	PNPA-3A					683:689	PNPA-3A	683:689	PNPA-3A	683:689	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	0	27	theme	polysaccharides	37:51	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of water-soluble polysaccharides	0:51	Structural analysis of water-soluble polysaccharides isolated from Panax notoginseng.					
32240740	4	28	theme	nuclear	478:484	arg1	resonance					495:503	nuclear magnetic resonance	478:503	nuclear magnetic resonance	478:503	Monosaccharide composition, enzymatic hydrolysis, nuclear magnetic resonance and methylation analysis were combined to characterize their structures.					
32240740	7	29	theme	different	903:911	arg1	ratios					913:918	different ratios	903:918	different ratios of RG-I	903:926	PNPA-1B, PNPA-2B and PNPA-3B consisted of homogalacturonan (HG) as major domains, together with different ratios of RG-I and rhamnogalacturonan II (RG-II) domains.					
32240740	5	30	contain	contained	605:613	arg1	PNPA-1A					578:584	PNPA-1A	578:584	PNPA-1A	578:584	PNPA-1A and PNPA-2A mainly contained 1,4-β-D-galactans, 1,5-α-L-arabinan and arabinogalactan II (AG-II).					
32240740	5	30	contain	contained	605:613	arg1	PNPA-2A					590:596	PNPA-2A	590:596	PNPA-2A	590:596	PNPA-1A and PNPA-2A mainly contained 1,4-β-D-galactans, 1,5-α-L-arabinan and arabinogalactan II (AG-II).					
32240740	5	30	contain	contained	605:613	arg2	1,4-β-D-galactans					615:631	1,4-β-D-galactans	615:631	1,4-β-D-galactans	615:631	PNPA-1A and PNPA-2A mainly contained 1,4-β-D-galactans, 1,5-α-L-arabinan and arabinogalactan II (AG-II).					
32240740	5	30	contain	contained	605:613	arg2	1,5-α-L-arabinan					634:649	1,5-α-L-arabinan	634:649	1,5-α-L-arabinan	634:649	PNPA-1A and PNPA-2A mainly contained 1,4-β-D-galactans, 1,5-α-L-arabinan and arabinogalactan II (AG-II).					
32240740	5	30	contain	contained	605:613	arg2	AG-II					675:679	AG-II	675:679	AG-II	675:679	PNPA-1A and PNPA-2A mainly contained 1,4-β-D-galactans, 1,5-α-L-arabinan and arabinogalactan II (AG-II).					
32240740	5	30	contain	contained	605:613	arg2	arabinogalactan					655:669	arabinogalactan II	655:672	arabinogalactan II (AG-II)	655:680	PNPA-1A and PNPA-2A mainly contained 1,4-β-D-galactans, 1,5-α-L-arabinan and arabinogalactan II (AG-II).					
32240740	0	31	theme	Panax	67:71	arg1	notoginseng					73:83	Panax notoginseng	67:83	Panax notoginseng	67:83	Structural analysis of water-soluble polysaccharides isolated from Panax notoginseng.					
32240740	1	32	theme	traditional	121:131	arg1	medicine					141:148	a widely used traditional Chinese medicine	107:148	a widely used traditional Chinese medicine	107:148	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	1	32	theme	traditional	121:131	arg1	notoginseng					92:102	Panax notoginseng	86:102	Panax notoginseng	86:102	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	3	33	theme	pectin	338:343	arg1	PNPA-3A					392:398	PNPA-3A	392:398	PNPA-3A	392:398	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	33	theme	pectin	338:343	arg1	PNPA-1B					365:371	PNPA-1B	365:371	PNPA-1B	365:371	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	33	theme	pectin	338:343	arg1	PNPA-2A					374:380	PNPA-2A	374:380	PNPA-2A	374:380	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	33	theme	pectin	338:343	arg1	PNPA-3B					404:410	PNPA-3B	404:410	PNPA-3B	404:410	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	33	theme	pectin	338:343	arg1	fractions					345:353	six pectin fractions	334:353	six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B)	334:411	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	33	theme	pectin	338:343	arg1	PNPA-1A					356:362	PNPA-1A	356:362	PNPA-1A	356:362	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	3	33	theme	pectin	338:343	arg1	PNPA-2B					383:389	PNPA-2B	383:389	PNPA-2B	383:389	One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained.					
32240740	4	34	theme	Monosaccharide	428:441	arg1	composition					443:453	Monosaccharide composition	428:453	Monosaccharide composition	428:453	Monosaccharide composition, enzymatic hydrolysis, nuclear magnetic resonance and methylation analysis were combined to characterize their structures.					
32240740	2	35	theme	Panax	242:246	arg1	notoginseng					248:258	Panax notoginseng	242:258	Panax notoginseng	242:258	In this work, water-soluble polysaccharides from Panax notoginseng were isolated and fractionated.					
32240740	1	36	theme	Chinese	133:139	arg1	medicine					141:148	a widely used traditional Chinese medicine	107:148	a widely used traditional Chinese medicine	107:148	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	1	36	theme	Chinese	133:139	arg1	notoginseng					92:102	Panax notoginseng	86:102	Panax notoginseng	86:102	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	6	37	theme	rhamnogalacturonan	705:722	arg1	pectin					738:743	a typical rhamnogalacturonan I (RG-I) type pectin	695:743	a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	695:804	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	6	37	theme	rhamnogalacturonan	705:722	arg1	PNPA-3A					683:689	PNPA-3A	683:689	PNPA-3A	683:689	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	6	38	theme	typical	697:703	arg1	pectin					738:743	a typical rhamnogalacturonan I (RG-I) type pectin	695:743	a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	695:804	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	6	38	theme	typical	697:703	arg1	PNPA-3A					683:689	PNPA-3A	683:689	PNPA-3A	683:689	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	2	39	theme	water-soluble	207:219	arg1	polysaccharides					221:235	water-soluble polysaccharides	207:235	water-soluble polysaccharides from Panax notoginseng	207:258	In this work, water-soluble polysaccharides from Panax notoginseng were isolated and fractionated.					
32240740	7	40	dep	together	889:896	arg1	with					898:901	with	898:901	with	898:901	PNPA-1B, PNPA-2B and PNPA-3B consisted of homogalacturonan (HG) as major domains, together with different ratios of RG-I and rhamnogalacturonan II (RG-II) domains.					
32240740	6	41	theme	side	794:797	arg1	chains					799:804	1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	750:804	1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	750:804	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	6	42	theme	1,5/1,3,5-α-L-arabinan	771:792	arg1	chains					799:804	1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	750:804	1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	750:804	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
32240740	1	43	theme	extensive	158:166	arg1	effects					184:190	extensive pharmacological effects	158:190	extensive pharmacological effects	158:190	Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects.					
32240740	6	44	theme	1,4-β-D-galactan	750:765	arg1	chains					799:804	1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	750:804	1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains	750:804	PNPA-3A was a typical rhamnogalacturonan I (RG-I) type pectin with 1,4-β-D-galactan and 1,5/1,3,5-α-L-arabinan side chains.					
33709491	0	0	theme	Binding	99:105	arg1	Profiles					107:114	Different Binding Profiles	89:114	Different Binding Profiles of Monoclonal Antibodies	89:139	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.					
33709491	5	1	gly	glycoproteins	708:720	arg1	glycoproteins					708:720	RBD glycoproteins	704:720	RBD glycoproteins with structurally defined glycans	704:754	Unfortunately, there is no viable method for preparing RBD glycoproteins with structurally defined glycans.					
33709491	4	2	theme	different	590:598	arg1	domains					613:619	different carbohydrate domains	590:619	different carbohydrate domains	590:619	Investigating the role of different carbohydrate domains is of paramount importance.					
33709491	0	3	theme	Different	89:97	arg1	Profiles					107:114	Different Binding Profiles	89:114	Different Binding Profiles of Monoclonal Antibodies	89:139	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.					
33709491	6	4	theme	glycosylated	844:855	arg1	RBDs					857:860	six glycosylated RBDs	840:860	six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343	840:921	Herein we describe a highly efficient and scalable strategy for the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343.					
33709491	1	5	attach	attaches	153:160	arg1	receptor					174:181	its host receptor	165:181	its host receptor	165:181	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	1	5	attach	attaches	153:160	arg1	enzyme					207:212	angiotensin-converting enzyme 2	184:214	angiotensin-converting enzyme 2 (ACE2)	184:221	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	1	5	attach	attaches	153:160	arg2	SARS-CoV-2					142:151	SARS-CoV-2	142:151	SARS-CoV-2	142:151	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	5	6	theme	viable	676:681	arg1	method					683:688	no viable method	673:688	no viable method for preparing RBD glycoproteins with structurally defined glycans	673:754	Unfortunately, there is no viable method for preparing RBD glycoproteins with structurally defined glycans.					
33709491	7	7	theme	protein	999:1005	arg1	engineering					1007:1017	recombinant protein engineering	987:1017	recombinant protein engineering	987:1017	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering provides a robust route to decipher carbohydrate structure-function relationships.					
33709491	3	8	theme	RBD-based	544:552	arg1	vaccines					554:561	RBD-based vaccines	544:561	RBD-based vaccines	544:561	However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.					
33709491	4	9	theme	domains	613:619	arg1	role					582:585	the role	578:585	the role of different carbohydrate domains	578:619	Investigating the role of different carbohydrate domains is of paramount importance.					
33709491	7	10	theme	carbohydrate	1055:1066	arg1	relationships					1087:1099	carbohydrate structure-function relationships	1055:1099	carbohydrate structure-function relationships	1055:1099	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering provides a robust route to decipher carbohydrate structure-function relationships.					
33709491	7	11	theme	synthesis	973:981	arg1	combination					926:936	A combination	924:936	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering	924:1017	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering provides a robust route to decipher carbohydrate structure-function relationships.					
33709491	3	12	theme	vaccines	554:561	arg1	integrity					531:539	the immunogenic integrity	515:539	the immunogenic integrity of RBD-based vaccines	515:561	However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.					
33709491	3	13	theme	incomplete	471:480	arg1	effect					497:502	an incomplete neutralization effect	468:502	an incomplete neutralization effect	468:502	However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.					
33709491	0	14	theme	Antibodies	130:139	arg1	Profiles					107:114	Different Binding Profiles	89:114	Different Binding Profiles of Monoclonal Antibodies	89:139	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.					
33709491	1	15	theme	angiotensin-converting	184:205	arg1	receptor					174:181	its host receptor	165:181	its host receptor	165:181	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	1	15	theme	angiotensin-converting	184:205	arg1	enzyme					207:212	angiotensin-converting enzyme 2	184:214	angiotensin-converting enzyme 2 (ACE2)	184:221	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	1	15	theme	angiotensin-converting	184:205	arg1	ACE2					217:220	ACE2	217:220	ACE2	217:220	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	0	16	theme	Monoclonal	119:128	arg1	Antibodies					130:139	Monoclonal Antibodies	119:139	Monoclonal Antibodies	119:139	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.					
33709491	3	17	theme	RBD	441:443	arg1	glycoforms					445:454	RBD glycoforms	441:454	RBD glycoforms	441:454	However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.					
33709491	0	18	theme	Homogeneous	10:20	arg1	Glycoforms					22:31	Synthetic Homogeneous Glycoforms	0:31	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain	0:79	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.					
33709491	2	19	gly	glycoprotein	292:303	arg1	glycoprotein					292:303	The RBD glycoprotein	284:303	The RBD glycoprotein	284:303	The RBD glycoprotein is a critical target for the development of neutralizing antibodies and vaccines against SARS-CoV-2.					
33709491	2	19	gly	glycoprotein	292:303	arg1	target					319:324	a critical target	308:324	a critical target for the development of neutralizing antibodies and vaccines against SARS-CoV-2	308:403	The RBD glycoprotein is a critical target for the development of neutralizing antibodies and vaccines against SARS-CoV-2.					
33709491	7	20	theme	peptide	965:971	arg1	synthesis					973:981	peptide synthesis	965:981	peptide synthesis	965:981	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering provides a robust route to decipher carbohydrate structure-function relationships.					
33709491	3	21	theme	high	419:422	arg1	heterogeneity					424:436	the high heterogeneity	415:436	the high heterogeneity of RBD glycoforms	415:454	However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.					
33709491	0	22	theme	Synthetic	0:8	arg1	Glycoforms					22:31	Synthetic Homogeneous Glycoforms	0:31	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain	0:79	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.					
33709491	1	23	theme	spike	269:273	arg1	protein					275:281	the spike protein	265:281	the spike protein	265:281	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	7	24	theme	modern	941:946	arg1	oligosaccharide					948:962	modern oligosaccharide	941:962	modern oligosaccharide	941:962	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering provides a robust route to decipher carbohydrate structure-function relationships.					
33709491	1	25	theme	protein	275:281	arg1	domain					249:254	the receptor-binding domain	228:254	the receptor-binding domain (RBD) of the spike protein	228:281	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	1	25	theme	protein	275:281	arg1	protein					275:281	the spike protein	265:281	the spike protein	265:281	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	1	25	theme	protein	275:281	arg1	RBD					257:259	RBD	257:259	RBD	257:259	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	6	26	theme	scalable	799:806	arg1	strategy					808:815	a highly efficient and scalable strategy	776:815	a highly efficient and scalable strategy for the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343	776:921	Herein we describe a highly efficient and scalable strategy for the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343.					
33709491	7	27	theme	recombinant	987:997	arg1	engineering					1007:1017	recombinant protein engineering	987:1017	recombinant protein engineering	987:1017	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering provides a robust route to decipher carbohydrate structure-function relationships.					
33709491	3	28	theme	immunogenic	519:529	arg1	integrity					531:539	the immunogenic integrity	515:539	the immunogenic integrity of RBD-based vaccines	515:561	However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.					
33709491	7	29	theme	structure-function	1068:1085	arg1	relationships					1087:1099	carbohydrate structure-function relationships	1055:1099	carbohydrate structure-function relationships	1055:1099	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering provides a robust route to decipher carbohydrate structure-function relationships.					
33709491	2	30	theme	RBD	288:290	arg1	glycoprotein					292:303	The RBD glycoprotein	284:303	The RBD glycoprotein	284:303	The RBD glycoprotein is a critical target for the development of neutralizing antibodies and vaccines against SARS-CoV-2.					
33709491	2	30	theme	RBD	288:290	arg1	target					319:324	a critical target	308:324	a critical target for the development of neutralizing antibodies and vaccines against SARS-CoV-2	308:403	The RBD glycoprotein is a critical target for the development of neutralizing antibodies and vaccines against SARS-CoV-2.					
33709491	6	31	gly	glycoforms	888:897	arg1	structure					878:886	defined structure glycoforms	870:897	defined structure glycoforms	870:897	Herein we describe a highly efficient and scalable strategy for the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343.					
33709491	0	32	theme	SARS-CoV-2	40:49	arg1	Domain					74:79	the SARS-CoV-2 Spike Receptor-Binding Domain	36:79	the SARS-CoV-2 Spike Receptor-Binding Domain	36:79	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.					
33709491	5	33	theme	defined	740:746	arg1	glycans					748:754	structurally defined glycans	727:754	structurally defined glycans	727:754	Unfortunately, there is no viable method for preparing RBD glycoproteins with structurally defined glycans.					
33709491	3	34	theme	glycoforms	445:454	arg1	heterogeneity					424:436	the high heterogeneity	415:436	the high heterogeneity of RBD glycoforms	415:454	However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.					
33709491	0	35	theme	Receptor-Binding	57:72	arg1	Domain					74:79	the SARS-CoV-2 Spike Receptor-Binding Domain	36:79	the SARS-CoV-2 Spike Receptor-Binding Domain	36:79	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.					
33709491	6	36	gly	glycosylated	844:855	arg1	RBDs					857:860	six glycosylated RBDs	840:860	six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343	840:921	Herein we describe a highly efficient and scalable strategy for the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343.					
33709491	6	37	theme	efficient	785:793	arg1	strategy					808:815	a highly efficient and scalable strategy	776:815	a highly efficient and scalable strategy for the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343	776:921	Herein we describe a highly efficient and scalable strategy for the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343.					
33709491	3	38	theme	neutralization	482:495	arg1	effect					497:502	an incomplete neutralization effect	468:502	an incomplete neutralization effect	468:502	However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.					
33709491	0	39	theme	Spike	51:55	arg1	Domain					74:79	the SARS-CoV-2 Spike Receptor-Binding Domain	36:79	the SARS-CoV-2 Spike Receptor-Binding Domain	36:79	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.					
33709491	6	40	theme	structure	878:886	arg1	glycoforms					888:897	defined structure glycoforms	870:897	defined structure glycoforms	870:897	Herein we describe a highly efficient and scalable strategy for the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343.					
33709491	3	41	gly	glycoforms	445:454	arg1	RBD					441:443	RBD glycoforms	441:454	RBD glycoforms	441:454	However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.					
33709491	7	42	theme	oligosaccharide	948:962	arg1	combination					926:936	A combination	924:936	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering	924:1017	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering provides a robust route to decipher carbohydrate structure-function relationships.					
33709491	5	43	theme	RBD	704:706	arg1	glycoproteins					708:720	RBD glycoproteins	704:720	RBD glycoproteins with structurally defined glycans	704:754	Unfortunately, there is no viable method for preparing RBD glycoproteins with structurally defined glycans.					
33709491	6	44	theme	defined	870:876	arg1	glycoforms					888:897	defined structure glycoforms	870:897	defined structure glycoforms	870:897	Herein we describe a highly efficient and scalable strategy for the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343.					
33709491	2	45	theme	critical	310:317	arg1	glycoprotein					292:303	The RBD glycoprotein	284:303	The RBD glycoprotein	284:303	The RBD glycoprotein is a critical target for the development of neutralizing antibodies and vaccines against SARS-CoV-2.					
33709491	2	45	theme	critical	310:317	arg1	target					319:324	a critical target	308:324	a critical target for the development of neutralizing antibodies and vaccines against SARS-CoV-2	308:403	The RBD glycoprotein is a critical target for the development of neutralizing antibodies and vaccines against SARS-CoV-2.					
33709491	1	46	theme	receptor-binding	232:247	arg1	domain					249:254	the receptor-binding domain	228:254	the receptor-binding domain (RBD) of the spike protein	228:281	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	1	46	theme	receptor-binding	232:247	arg1	protein					275:281	the spike protein	265:281	the spike protein	265:281	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	1	46	theme	receptor-binding	232:247	arg1	RBD					257:259	RBD	257:259	RBD	257:259	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	6	47	theme	RBDs	857:860	arg1	preparation					825:835	the preparation	821:835	the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343	821:921	Herein we describe a highly efficient and scalable strategy for the preparation of six glycosylated RBDs bearing defined structure glycoforms at T323, N331, and N343.					
33709491	3	48	gly	heterogeneity	424:436	arg1	glycoforms					445:454	RBD glycoforms	441:454	RBD glycoforms	441:454	However, the high heterogeneity of RBD glycoforms may lead to an incomplete neutralization effect and impact the immunogenic integrity of RBD-based vaccines.					
33709491	7	49	theme	robust	1030:1035	arg1	route					1037:1041	a robust route	1028:1041	a robust route to decipher carbohydrate structure-function relationships	1028:1099	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering provides a robust route to decipher carbohydrate structure-function relationships.					
33709491	5	50	with	glycoproteins	708:720	arg1	glycans					748:754	structurally defined glycans	727:754	structurally defined glycans	727:754	Unfortunately, there is no viable method for preparing RBD glycoproteins with structurally defined glycans.					
33709491	4	51	theme	carbohydrate	600:611	arg1	domains					613:619	different carbohydrate domains	590:619	different carbohydrate domains	590:619	Investigating the role of different carbohydrate domains is of paramount importance.					
33709491	0	52	theme	Domain	74:79	arg1	Glycoforms					22:31	Synthetic Homogeneous Glycoforms	0:31	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain	0:79	Synthetic Homogeneous Glycoforms of the SARS-CoV-2 Spike Receptor-Binding Domain Reveals Different Binding Profiles of Monoclonal Antibodies.					
33709491	7	53	theme	engineering	1007:1017	arg1	combination					926:936	A combination	924:936	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering	924:1017	A combination of modern oligosaccharide, peptide synthesis and recombinant protein engineering provides a robust route to decipher carbohydrate structure-function relationships.					
33709491	1	54	theme	host	169:172	arg1	receptor					174:181	its host receptor	165:181	its host receptor	165:181	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	1	54	theme	host	169:172	arg1	enzyme					207:212	angiotensin-converting enzyme 2	184:214	angiotensin-converting enzyme 2 (ACE2)	184:221	SARS-CoV-2 attaches to its host receptor, angiotensin-converting enzyme 2 (ACE2), via the receptor-binding domain (RBD) of the spike protein.					
33709491	4	55	theme	paramount	627:635	arg1	importance					637:646	paramount importance	627:646	paramount importance	627:646	Investigating the role of different carbohydrate domains is of paramount importance.					
34774592	0	0	theme	quercetin	87:95	arg1	delivery					97:104	quercetin delivery	87:104	quercetin delivery	87:104	Enhancing the stability of zein/fucoidan composite nanoparticles with calcium ions for quercetin delivery.					
34774592	5	1	with	polysaccharides	937:951	arg1	Ca2+					964:967	added Ca2+	958:967	added Ca2+	958:967	The composite nanosystems based on alcohol-soluble proteins and anionic polysaccharides with added Ca2+ can be potentially applied for the delivery of active substances.					
34774592	2	2	dep	concentrations	279:292	arg1	0-3.0 mM					269:276	different calcium ion (Ca2+, 0-3.0 mM) concentrations	240:292	0-3.0 mM	269:276	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	3	3	theme	hydrogen	556:563	arg1	forces					616:621	the main forces	607:621	the main forces underlying the formation of composite nanoparticles	607:673	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	3	theme	hydrogen	556:563	arg1	interactions					542:553	Electrostatic interactions	528:553	Electrostatic interactions	528:553	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	3	theme	hydrogen	556:563	arg1	bonding					565:571	hydrogen bonding	556:571	hydrogen bonding	556:571	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	3	theme	hydrogen	556:563	arg1	interactions					590:601	hydrophobic interactions	578:601	hydrophobic interactions	578:601	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	4	4	theme	composite	755:763	arg1	nanoparticles					765:777	the composite nanoparticles	751:777	the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM)	751:862	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	4	5	theme	simulated	782:790	arg1	solutions					802:810	simulated digestive solutions	782:810	simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM)	782:862	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	2	6	theme	concentrations	279:292	arg1	effects					229:235	The effects	225:235	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin	225:360	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	4	7	theme	substances	735:744	arg1	release					713:719	improved release	704:719	improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM)	704:862	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	5	8	theme	added	958:962	arg1	Ca2+					964:967	added Ca2+	958:967	added Ca2+	958:967	The composite nanosystems based on alcohol-soluble proteins and anionic polysaccharides with added Ca2+ can be potentially applied for the delivery of active substances.					
34774592	5	9	theme	active	1016:1021	arg1	substances					1023:1032	active substances	1016:1032	active substances	1016:1032	The composite nanosystems based on alcohol-soluble proteins and anionic polysaccharides with added Ca2+ can be potentially applied for the delivery of active substances.					
34774592	2	10	from	effects	229:235	arg1	stability					301:309	the stability	297:309	the stability of the composite nanosystems loaded with quercetin	297:360	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	0	11	theme	nanoparticles	51:63	arg1	stability					14:22	the stability	10:22	the stability of zein/fucoidan composite nanoparticles with calcium ions for quercetin delivery	10:104	Enhancing the stability of zein/fucoidan composite nanoparticles with calcium ions for quercetin delivery.					
34774592	5	12	theme	substances	1023:1032	arg1	delivery					1004:1011	the delivery	1000:1011	the delivery of active substances	1000:1032	The composite nanosystems based on alcohol-soluble proteins and anionic polysaccharides with added Ca2+ can be potentially applied for the delivery of active substances.					
34774592	2	13	theme	ultraviolet	444:454	arg1	irradiation					462:472	ultraviolet light irradiation	444:472	ultraviolet light irradiation	444:472	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	13	theme	ultraviolet	444:454	arg1	conditions					391:400	different conditions	381:400	different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation)	381:473	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	3	14	theme	Electrostatic	528:540	arg1	bonding					565:571	hydrogen bonding	556:571	hydrogen bonding	556:571	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	14	theme	Electrostatic	528:540	arg1	interactions					542:553	Electrostatic interactions	528:553	Electrostatic interactions	528:553	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	14	theme	Electrostatic	528:540	arg1	forces					616:621	the main forces	607:621	the main forces underlying the formation of composite nanoparticles	607:673	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	14	theme	Electrostatic	528:540	arg1	interactions					590:601	hydrophobic interactions	578:601	hydrophobic interactions	578:601	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	4	15	from	nanoparticles	765:777	arg1	release					713:719	improved release	704:719	improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM)	704:862	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	4	15	from	nanoparticles	765:777	arg1	solutions					802:810	simulated digestive solutions	782:810	simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM)	782:862	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	3	16	theme	hydrophobic	578:588	arg1	bonding					565:571	hydrogen bonding	556:571	hydrogen bonding	556:571	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	16	theme	hydrophobic	578:588	arg1	interactions					542:553	Electrostatic interactions	528:553	Electrostatic interactions	528:553	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	16	theme	hydrophobic	578:588	arg1	forces					616:621	the main forces	607:621	the main forces underlying the formation of composite nanoparticles	607:673	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	16	theme	hydrophobic	578:588	arg1	interactions					590:601	hydrophobic interactions	578:601	hydrophobic interactions	578:601	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	5	17	theme	alcohol-soluble	900:914	arg1	proteins					916:923	alcohol-soluble proteins	900:923	alcohol-soluble proteins	900:923	The composite nanosystems based on alcohol-soluble proteins and anionic polysaccharides with added Ca2+ can be potentially applied for the delivery of active substances.					
34774592	4	18	theme	digestive	792:800	arg1	solutions					802:810	simulated digestive solutions	782:810	simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM)	782:862	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	1	19	theme	antisolvent	191:201	arg1	method					217:222	the antisolvent precipitation method	187:222	the antisolvent precipitation method	187:222	In this study, zein and fucoidan-based composite nanoparticles were prepared by the antisolvent precipitation method.					
34774592	3	20	theme	main	611:614	arg1	bonding					565:571	hydrogen bonding	556:571	hydrogen bonding	556:571	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	20	theme	main	611:614	arg1	interactions					542:553	Electrostatic interactions	528:553	Electrostatic interactions	528:553	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	20	theme	main	611:614	arg1	forces					616:621	the main forces	607:621	the main forces underlying the formation of composite nanoparticles	607:673	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	3	20	theme	main	611:614	arg1	interactions					590:601	hydrophobic interactions	578:601	hydrophobic interactions	578:601	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	2	21	theme	salt	420:423	arg1	concentration					425:437	salt concentration	420:437	salt concentration	420:437	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	21	theme	salt	420:423	arg1	conditions					391:400	different conditions	381:400	different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation)	381:473	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	4	22	theme	improved	704:711	arg1	release					713:719	improved release	704:719	improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM)	704:862	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	0	23	theme	composite	41:49	arg1	nanoparticles					51:63	zein/fucoidan composite nanoparticles	27:63	zein/fucoidan composite nanoparticles	27:63	Enhancing the stability of zein/fucoidan composite nanoparticles with calcium ions for quercetin delivery.					
34774592	2	24	theme	calcium	250:256	arg1	concentrations					279:292	different calcium ion (Ca2+, 0-3.0 mM) concentrations	240:292	concentrations	279:292	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	25	theme	different	381:389	arg1	irradiation					462:472	ultraviolet light irradiation	444:472	ultraviolet light irradiation	444:472	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	25	theme	different	381:389	arg1	concentration					425:437	salt concentration	420:437	salt concentration	420:437	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	25	theme	different	381:389	arg1	pH					403:404	pH	403:404	pH	403:404	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	25	theme	different	381:389	arg1	conditions					391:400	different conditions	381:400	different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation)	381:473	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	25	theme	different	381:389	arg1	temperature					407:417	temperature	407:417	temperature	407:417	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	5	26	theme	composite	869:877	arg1	nanosystems					879:889	The composite nanosystems	865:889	The composite nanosystems based on alcohol-soluble proteins and anionic polysaccharides with added Ca2+	865:967	The composite nanosystems based on alcohol-soluble proteins and anionic polysaccharides with added Ca2+ can be potentially applied for the delivery of active substances.					
34774592	0	27	theme	zein/fucoidan	27:39	arg1	nanoparticles					51:63	zein/fucoidan composite nanoparticles	27:63	zein/fucoidan composite nanoparticles	27:63	Enhancing the stability of zein/fucoidan composite nanoparticles with calcium ions for quercetin delivery.					
34774592	3	28	theme	composite	651:659	arg1	nanoparticles					661:673	composite nanoparticles	651:673	composite nanoparticles	651:673	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	2	29	theme	different	240:248	arg1	concentrations					279:292	different calcium ion (Ca2+, 0-3.0 mM) concentrations	240:292	concentrations	279:292	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	0	30	theme	calcium	70:76	arg1	ions					78:81	calcium ions	70:81	calcium ions for quercetin delivery	70:104	Enhancing the stability of zein/fucoidan composite nanoparticles with calcium ions for quercetin delivery.					
34774592	0	31	with	stability	14:22	arg1	ions					78:81	calcium ions	70:81	calcium ions for quercetin delivery	70:104	Enhancing the stability of zein/fucoidan composite nanoparticles with calcium ions for quercetin delivery.					
34774592	4	32	theme	active	728:733	arg1	substances					735:744	the active substances	724:744	the active substances	724:744	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	2	33	theme	light	456:460	arg1	irradiation					462:472	ultraviolet light irradiation	444:472	ultraviolet light irradiation	444:472	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	33	theme	light	456:460	arg1	conditions					391:400	different conditions	381:400	different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation)	381:473	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	5	34	with	proteins	916:923	arg1	Ca2+					964:967	added Ca2+	958:967	added Ca2+	958:967	The composite nanosystems based on alcohol-soluble proteins and anionic polysaccharides with added Ca2+ can be potentially applied for the delivery of active substances.					
34774592	3	35	theme	nanoparticles	661:673	arg1	formation					638:646	the formation	634:646	the formation of composite nanoparticles	634:673	Electrostatic interactions, hydrogen bonding, and hydrophobic interactions are the main forces underlying the formation of composite nanoparticles.					
34774592	4	36	theme	Ca2+	692:695	arg1	addition					680:687	The addition	676:687	The addition of Ca2+	676:695	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	2	37	dep	conditions	391:400	arg1	irradiation					462:472	ultraviolet light irradiation	444:472	ultraviolet light irradiation	444:472	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	37	dep	conditions	391:400	arg1	concentration					425:437	salt concentration	420:437	salt concentration	420:437	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	37	dep	conditions	391:400	arg1	pH					403:404	pH	403:404	pH	403:404	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	37	dep	conditions	391:400	arg1	conditions					391:400	different conditions	381:400	different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation)	381:473	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	37	dep	conditions	391:400	arg1	temperature					407:417	temperature	407:417	temperature	407:417	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	1	38	theme	zein	122:125	arg1	nanoparticles					156:168	zein and fucoidan-based composite nanoparticles	122:168	zein and fucoidan-based composite nanoparticles	122:168	In this study, zein and fucoidan-based composite nanoparticles were prepared by the antisolvent precipitation method.					
34774592	2	39	theme	Ca2+	263:266	arg1	concentrations					279:292	different calcium ion (Ca2+, 0-3.0 mM) concentrations	240:292	concentrations	279:292	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	40	theme	composite	318:326	arg1	nanosystems					328:338	the composite nanosystems	314:338	the composite nanosystems loaded with quercetin	314:360	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	41	theme	nanosystems	328:338	arg1	stability					301:309	the stability	297:309	the stability of the composite nanosystems loaded with quercetin	297:360	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	5	42	theme	anionic	929:935	arg1	polysaccharides					937:951	anionic polysaccharides	929:951	anionic polysaccharides	929:951	The composite nanosystems based on alcohol-soluble proteins and anionic polysaccharides with added Ca2+ can be potentially applied for the delivery of active substances.					
34774592	4	43	theme	Ca2+	833:836	arg1	1.5 mM					856:861	1.5 mM	856:861	1.5 mM	856:861	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	4	43	theme	Ca2+	833:836	arg1	concentration					838:850	the Ca2+ concentration	829:850	the Ca2+ concentration	829:850	The addition of Ca2+ led to improved release of the active substances from the composite nanoparticles in simulated digestive solutions (especially when the Ca2+ concentration was 1.5 mM).					
34774592	1	44	theme	precipitation	203:215	arg1	method					217:222	the antisolvent precipitation method	187:222	the antisolvent precipitation method	187:222	In this study, zein and fucoidan-based composite nanoparticles were prepared by the antisolvent precipitation method.					
34774592	1	45	theme	fucoidan-based	131:144	arg1	nanoparticles					156:168	zein and fucoidan-based composite nanoparticles	122:168	zein and fucoidan-based composite nanoparticles	122:168	In this study, zein and fucoidan-based composite nanoparticles were prepared by the antisolvent precipitation method.					
34774592	2	46	theme	composite	484:492	arg1	nanoparticles					494:506	the composite nanoparticles	480:506	the composite nanoparticles	480:506	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	2	47	theme	ion	258:260	arg1	concentrations					279:292	different calcium ion (Ca2+, 0-3.0 mM) concentrations	240:292	concentrations	279:292	The effects of different calcium ion (Ca2+, 0-3.0 mM) concentrations on the stability of the composite nanosystems loaded with quercetin were studied under different conditions (pH, temperature, salt concentration, and ultraviolet light irradiation), and the composite nanoparticles were characterized.					
34774592	1	48	theme	composite	146:154	arg1	nanoparticles					156:168	zein and fucoidan-based composite nanoparticles	122:168	zein and fucoidan-based composite nanoparticles	122:168	In this study, zein and fucoidan-based composite nanoparticles were prepared by the antisolvent precipitation method.					
33977864	0	0	theme	compounds	81:89	arg1	properties					31:40	optical and thermal properties	11:40	optical and thermal properties of natural polymer-based hemicellulose compounds	11:89	A study on optical and thermal properties of natural polymer-based hemicellulose compounds.					
33977864	4	1	theme	composite	872:880	arg1	films					904:908	pure or composite metal-doped synthetic films	864:908	pure or composite metal-doped synthetic films	864:908	The Refractive index of these films is also found high in the visible region in comparison to pure or composite metal-doped synthetic films.					
33977864	3	2	theme	use	735:737	arg1	materials					759:767	use synthetic polymeric materials	735:767	use synthetic polymeric materials	735:767	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	2	3	theme	natural	341:347	arg1	materials					349:357	these natural materials	335:357	these natural materials	335:357	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	5	4	theme	polymeric	1057:1065	arg1	films					1067:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	0	5	theme	hemicellulose	67:79	arg1	compounds					81:89	natural polymer-based hemicellulose compounds	45:89	natural polymer-based hemicellulose compounds	45:89	A study on optical and thermal properties of natural polymer-based hemicellulose compounds.					
33977864	0	6	from	study	2:6	arg1	properties					31:40	optical and thermal properties	11:40	optical and thermal properties of natural polymer-based hemicellulose compounds	11:89	A study on optical and thermal properties of natural polymer-based hemicellulose compounds.					
33977864	5	7	theme	Urbach	911:916	arg1	Eu					926:927	Eu	926:927	Eu	926:927	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	7	theme	Urbach	911:916	arg1	energy					918:923	Urbach energy	911:923	Urbach energy (Eu)	911:928	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	8	theme	energy	918:923	arg1	films					1067:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	3	9	theme	optical	696:702	arg1	conductivity					704:715	better optical conductivity	689:715	better optical conductivity	689:715	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	2	10	theme	thermal	520:526	arg1	stability					528:536	high thermal stability	515:536	high thermal stability	515:536	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	2	11	with	transparency	501:512	arg1	absorption					579:588	minimum light absorption	565:588	minimum light absorption	565:588	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	4	12	theme	synthetic	894:902	arg1	films					904:908	pure or composite metal-doped synthetic films	864:908	pure or composite metal-doped synthetic films	864:908	The Refractive index of these films is also found high in the visible region in comparison to pure or composite metal-doped synthetic films.					
33977864	5	13	theme	natural	1049:1055	arg1	films					1067:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	2	14	theme	high	515:518	arg1	stability					528:536	high thermal stability	515:536	high thermal stability	515:536	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	3	15	theme	synthetic	739:747	arg1	materials					759:767	use synthetic polymeric materials	735:767	use synthetic polymeric materials	735:767	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	4	16	theme	metal-doped	882:892	arg1	films					904:908	pure or composite metal-doped synthetic films	864:908	pure or composite metal-doped synthetic films	864:908	The Refractive index of these films is also found high in the visible region in comparison to pure or composite metal-doped synthetic films.					
33977864	5	17	theme	wavelength	975:984	arg1	films					1067:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	3	18	theme	polymeric	749:757	arg1	materials					759:767	use synthetic polymeric materials	735:767	use synthetic polymeric materials	735:767	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	5	19	theme	hemicellulose	1029:1041	arg1	λ0					987:988	λ0	987:988	λ0	987:988	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	19	theme	hemicellulose	1029:1041	arg1	Eu					926:927	Eu	926:927	Eu	926:927	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	19	theme	hemicellulose	1029:1041	arg1	S0					1017:1018	S0	1017:1018	S0	1017:1018	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	19	theme	hemicellulose	1029:1041	arg1	energy					918:923	Urbach energy	911:923	Urbach energy (Eu)	911:928	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	19	theme	hemicellulose	1029:1041	arg1	energy					942:947	Dispersion energy	931:947	Dispersion energy (Ed)	931:952	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	19	theme	hemicellulose	1029:1041	arg1	strength					1008:1015	oscillation strength	996:1015	oscillation strength(S0) of this hemicellulose	996:1041	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	19	theme	hemicellulose	1029:1041	arg1	wavelength					975:984	Average oscillation wavelength	955:984	Average oscillation wavelength (λ0)	955:989	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	19	theme	hemicellulose	1029:1041	arg1	Ed					950:951	Ed	950:951	Ed	950:951	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	4	20	theme	films	800:804	arg1	index					785:789	The Refractive index	770:789	The Refractive index of these films	770:804	The Refractive index of these films is also found high in the visible region in comparison to pure or composite metal-doped synthetic films.					
33977864	2	21	theme	fiber	384:388	arg1	optics					390:395	fiber optics	384:395	fiber optics	384:395	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	2	22	theme	transparency	501:512	arg1	need					483:486	need	483:486	need of efficient transparency, high thermal stability and good conductivity with minimum light absorption	483:588	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	5	23	theme	oscillation	963:973	arg1	λ0					987:988	λ0	987:988	λ0	987:988	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	23	theme	oscillation	963:973	arg1	wavelength					975:984	Average oscillation wavelength	955:984	Average oscillation wavelength (λ0)	955:989	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	2	24	theme	infrastructure	439:452	arg1	applications					454:465	human transplantation infrastructure applications	417:465	human transplantation infrastructure applications	417:465	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	2	25	contain	have	359:362	arg1	materials					349:357	these natural materials	335:357	these natural materials	335:357	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	2	25	contain	have	359:362	arg2	potential					371:379	strong potential	364:379	strong potential	364:379	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	4	26	theme	pure	864:867	arg1	films					904:908	pure or composite metal-doped synthetic films	864:908	pure or composite metal-doped synthetic films	864:908	The Refractive index of these films is also found high in the visible region in comparison to pure or composite metal-doped synthetic films.					
33977864	2	27	theme	efficient	491:499	arg1	transparency					501:512	efficient transparency	491:512	efficient transparency	491:512	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	5	28	theme	Dispersion	931:940	arg1	Ed					950:951	Ed	950:951	Ed	950:951	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	28	theme	Dispersion	931:940	arg1	energy					942:947	Dispersion energy	931:947	Dispersion energy (Ed)	931:952	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	2	29	theme	transplantation	423:437	arg1	applications					454:465	human transplantation infrastructure applications	417:465	human transplantation infrastructure applications	417:465	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	1	30	theme	optical	260:266	arg1	properties					268:277	their optical properties	254:277	their optical properties	254:277	Films of husks of Plantango ovate, Cydonia oblonga, Mimosa pudica, Cochlospermum religiosum were prepared, delignified without protein and cellulose content, and their optical properties were evaluated.					
33977864	5	31	theme	energy	942:947	arg1	films					1067:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	2	32	theme	strong	364:369	arg1	potential					371:379	strong potential	364:379	strong potential	364:379	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	0	33	theme	optical	11:17	arg1	properties					31:40	optical and thermal properties	11:40	optical and thermal properties of natural polymer-based hemicellulose compounds	11:89	A study on optical and thermal properties of natural polymer-based hemicellulose compounds.					
33977864	2	34	theme	conductivity	547:558	arg1	need					483:486	need	483:486	need of efficient transparency, high thermal stability and good conductivity with minimum light absorption	483:588	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	5	35	theme	strength	1008:1015	arg1	films					1067:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	2	36	theme	UV-Vis	295:300	arg1	analysis					312:319	UV-Vis, FTIR TGA analysis	295:319	UV-Vis, FTIR TGA analysis	295:319	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	3	37	theme	natural	597:603	arg1	films					615:619	These natural polymeric films	591:619	These natural polymeric films	591:619	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	2	38	theme	good	542:545	arg1	conductivity					547:558	good conductivity	542:558	good conductivity	542:558	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	3	39	theme	optical	661:667	arg1	values					678:683	significant direct and indirect optical band gap values	629:683	significant direct and indirect optical band gap values	629:683	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	1	40	theme	husks	101:105	arg1	Films					92:96	Films	92:96	Films of husks of Plantango ovate, Cydonia oblonga, Mimosa pudica, Cochlospermum religiosum	92:182	Films of husks of Plantango ovate, Cydonia oblonga, Mimosa pudica, Cochlospermum religiosum were prepared, delignified without protein and cellulose content, and their optical properties were evaluated.					
33977864	2	41	theme	contact	398:404	arg1	lenses					406:411	contact lenses	398:411	contact lenses	398:411	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	0	42	theme	thermal	23:29	arg1	properties					31:40	optical and thermal properties	11:40	optical and thermal properties of natural polymer-based hemicellulose compounds	11:89	A study on optical and thermal properties of natural polymer-based hemicellulose compounds.					
33977864	3	43	theme	band	669:672	arg1	values					678:683	significant direct and indirect optical band gap values	629:683	significant direct and indirect optical band gap values	629:683	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	1	44	dep	Cochlospermum	159:171	arg1	religiosum					173:182	Cochlospermum religiosum	159:182	Cochlospermum religiosum	159:182	Films of husks of Plantango ovate, Cydonia oblonga, Mimosa pudica, Cochlospermum religiosum were prepared, delignified without protein and cellulose content, and their optical properties were evaluated.					
33977864	5	45	theme	such	1106:1109	arg1	materials					1119:1127	such optical materials	1106:1127	such optical materials which are green, organic, economical and compatible to human systems	1106:1196	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	2	46	theme	stability	528:536	arg1	need					483:486	need	483:486	need of efficient transparency, high thermal stability and good conductivity with minimum light absorption	483:588	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	5	47	theme	based	1043:1047	arg1	films					1067:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films	911:1071	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	2	48	theme	human	417:421	arg1	applications					454:465	human transplantation infrastructure applications	417:465	human transplantation infrastructure applications	417:465	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	4	49	from	region	840:845	arg1	high					820:823	high	820:823	high	820:823	The Refractive index of these films is also found high in the visible region in comparison to pure or composite metal-doped synthetic films.					
33977864	4	49	from	region	840:845	arg1	comparison					850:859	comparison	850:859	comparison to pure or composite metal-doped synthetic films	850:908	The Refractive index of these films is also found high in the visible region in comparison to pure or composite metal-doped synthetic films.					
33977864	4	50	theme	Refractive	774:783	arg1	index					785:789	The Refractive index	770:789	The Refractive index of these films	770:804	The Refractive index of these films is also found high in the visible region in comparison to pure or composite metal-doped synthetic films.					
33977864	5	51	theme	optical	1111:1117	arg1	materials					1119:1127	such optical materials	1106:1127	such optical materials which are green, organic, economical and compatible to human systems	1106:1196	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	3	52	theme	indirect	652:659	arg1	values					678:683	significant direct and indirect optical band gap values	629:683	significant direct and indirect optical band gap values	629:683	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	5	53	theme	oscillation	996:1006	arg1	S0					1017:1018	S0	1017:1018	S0	1017:1018	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	53	theme	oscillation	996:1006	arg1	strength					1008:1015	oscillation strength	996:1015	oscillation strength(S0) of this hemicellulose	996:1041	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	2	54	dep	UV-Vis	295:300	arg1	FTIR					303:306	FTIR	303:306	FTIR	303:306	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	2	55	theme	light	573:577	arg1	absorption					579:588	minimum light absorption	565:588	minimum light absorption	565:588	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	4	56	theme	visible	832:838	arg1	region					840:845	the visible region	828:845	the visible region in comparison to pure or composite metal-doped synthetic films	828:908	The Refractive index of these films is also found high in the visible region in comparison to pure or composite metal-doped synthetic films.					
33977864	5	57	theme	human	1184:1188	arg1	systems					1190:1196	human systems	1184:1196	human systems	1184:1196	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	2	58	theme	minimum	565:571	arg1	absorption					579:588	minimum light absorption	565:588	minimum light absorption	565:588	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	3	59	theme	better	689:694	arg1	conductivity					704:715	better optical conductivity	689:715	better optical conductivity	689:715	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	3	60	theme	significant	629:639	arg1	values					678:683	significant direct and indirect optical band gap values	629:683	significant direct and indirect optical band gap values	629:683	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	3	61	theme	polymeric	605:613	arg1	films					615:619	These natural polymeric films	591:619	These natural polymeric films	591:619	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	1	62	theme	ovate	120:124	arg1	husks					101:105	husks	101:105	husks of Plantango ovate, Cydonia oblonga, Mimosa pudica, Cochlospermum religiosum	101:182	Films of husks of Plantango ovate, Cydonia oblonga, Mimosa pudica, Cochlospermum religiosum were prepared, delignified without protein and cellulose content, and their optical properties were evaluated.					
33977864	2	63	with	stability	528:536	arg1	absorption					579:588	minimum light absorption	565:588	minimum light absorption	565:588	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	3	64	contain	possess	621:627	arg2	values					678:683	significant direct and indirect optical band gap values	629:683	significant direct and indirect optical band gap values	629:683	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	3	64	contain	possess	621:627	arg2	conductivity					704:715	better optical conductivity	689:715	better optical conductivity	689:715	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	3	64	contain	possess	621:627	arg1	films					615:619	These natural polymeric films	591:619	These natural polymeric films	591:619	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	3	65	theme	gap	674:676	arg1	values					678:683	significant direct and indirect optical band gap values	629:683	significant direct and indirect optical band gap values	629:683	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	3	66	theme	direct	641:646	arg1	values					678:683	significant direct and indirect optical band gap values	629:683	significant direct and indirect optical band gap values	629:683	These natural polymeric films possess significant direct and indirect optical band gap values and better optical conductivity than currently in use synthetic polymeric materials.					
33977864	4	67	from	high	820:823	arg1	region					840:845	the visible region	828:845	the visible region in comparison to pure or composite metal-doped synthetic films	828:908	The Refractive index of these films is also found high in the visible region in comparison to pure or composite metal-doped synthetic films.					
33977864	2	68	theme	TGA	308:310	arg1	analysis					312:319	UV-Vis, FTIR TGA analysis	295:319	UV-Vis, FTIR TGA analysis	295:319	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	2	69	with	conductivity	547:558	arg1	absorption					579:588	minimum light absorption	565:588	minimum light absorption	565:588	UV-Vis, FTIR TGA analysis revealed that these natural materials have strong potential in fiber optics, contact lenses and human transplantation infrastructure applications, where there is need of efficient transparency, high thermal stability and good conductivity with minimum light absorption.					
33977864	1	70	theme	protein	219:225	arg1	content					241:247	protein and cellulose content	219:247	content	241:247	Films of husks of Plantango ovate, Cydonia oblonga, Mimosa pudica, Cochlospermum religiosum were prepared, delignified without protein and cellulose content, and their optical properties were evaluated.					
33977864	0	71	theme	polymer-based	53:65	arg1	compounds					81:89	natural polymer-based hemicellulose compounds	45:89	natural polymer-based hemicellulose compounds	45:89	A study on optical and thermal properties of natural polymer-based hemicellulose compounds.					
33977864	5	72	theme	Average	955:961	arg1	λ0					987:988	λ0	987:988	λ0	987:988	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	5	72	theme	Average	955:961	arg1	wavelength					975:984	Average oscillation wavelength	955:984	Average oscillation wavelength (λ0)	955:989	Urbach energy (Eu), Dispersion energy (Ed), Average oscillation wavelength (λ0), and oscillation strength(S0) of this hemicellulose based natural polymeric films were found to be appropriate for such optical materials which are green, organic, economical and compatible to human systems.					
33977864	0	73	theme	natural	45:51	arg1	compounds					81:89	natural polymer-based hemicellulose compounds	45:89	natural polymer-based hemicellulose compounds	45:89	A study on optical and thermal properties of natural polymer-based hemicellulose compounds.					
33977864	1	74	dep	prepared	189:196	arg1	delignified					199:209	delignified	199:209	delignified without protein and cellulose content	199:247	Films of husks of Plantango ovate, Cydonia oblonga, Mimosa pudica, Cochlospermum religiosum were prepared, delignified without protein and cellulose content, and their optical properties were evaluated.					
33977864	1	75	theme	cellulose	231:239	arg1	content					241:247	protein and cellulose content	219:247	content	241:247	Films of husks of Plantango ovate, Cydonia oblonga, Mimosa pudica, Cochlospermum religiosum were prepared, delignified without protein and cellulose content, and their optical properties were evaluated.					
32071394	4	0	theme	spring	645:650	arg1	blooms					658:663	2009-2012 spring algal blooms	635:663	2009-2012 spring algal blooms in the southern North Sea	635:689	(class Flavobacteriia; known to include relevant polysaccharide-degraders) that were abundant during 2009-2012 spring algal blooms in the southern North Sea.					
32071394	5	1	theme	distinct	710:717	arg1	clades					732:737	six distinct Polaribacter clades	706:737	six distinct Polaribacter clades	706:737	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	9	2	theme	sulfated	1337:1344	arg1	xylan					1346:1350	sulfated xylan	1337:1350	sulfated xylan	1337:1350	Polaribacter 3-b responded late in blooms and had the capacity to utilize sulfated xylan.					
32071394	2	3	theme	microbial	358:366	arg1	composition					378:388	the microbial community composition	354:388	shaping the microbial community composition	346:388	Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood.					
32071394	10	4	theme	high	1379:1382	arg1	numbers					1384:1390	high numbers	1379:1390	high numbers of glycan degradation genes	1379:1418	Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms.					
32071394	2	5	from	role	338:341	arg1	shaping					346:352	shaping the microbial community composition	346:388	shaping the microbial community composition	346:388	Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood.					
32071394	8	6	contain	possessed	1192:1200	arg1	Polaribacter					1144:1155	Polaribacter 3-a	1144:1159	Polaribacter 3-a	1144:1159	Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential.					
32071394	8	6	contain	possessed	1192:1200	arg2	potential					1252:1260	a distinct sulfated α-glucoronomannan degradation potential	1202:1260	a distinct sulfated α-glucoronomannan degradation potential	1202:1260	Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential.					
32071394	11	7	theme	spring	1606:1611	arg1	blooms					1619:1624	North Sea spring algal blooms	1596:1624	North Sea spring algal blooms	1596:1624	These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.					
32071394	2	8	theme	complex	293:299	arg1	class					301:305	the most diverse and structurally complex class	259:305	the most diverse and structurally complex class of these substrates	259:325	Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood.					
32071394	6	9	theme	polysaccharide	977:990	arg1	niches					992:997	distinct polysaccharide niches	968:997	distinct polysaccharide niches	968:997	Four clades with distinct polysaccharide niches were dominating.					
32071394	11	10	theme	sympatric	1529:1537	arg1	clades					1552:1557	sympatric Polaribacter clades	1529:1557	sympatric Polaribacter clades	1529:1557	These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.					
32071394	1	11	theme	clades	193:198	arg1	growth					178:183	growth	178:183	growth of many clades of heterotrophic bacteria	178:224	Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria.					
32071394	8	12	from	2010	1183:1186	arg1	abundant					1166:1173	abundant	1166:1173	abundant	1166:1173	Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential.					
32071394	2	13	theme	substrates	316:325	arg1	class					301:305	the most diverse and structurally complex class	259:305	the most diverse and structurally complex class of these substrates	259:325	Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood.					
32071394	1	14	theme	heterotrophic	203:215	arg1	bacteria					217:224	heterotrophic bacteria	203:224	heterotrophic bacteria	203:224	Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria.					
32071394	11	15	theme	distinct	1566:1573	arg1	niches					1582:1587	distinct glycan niches	1566:1587	distinct glycan niches	1566:1587	These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.					
32071394	7	16	with	genomes	1084:1090	arg1	capacities					1132:1141	limited polysaccharide utilization capacities	1097:1141	limited polysaccharide utilization capacities	1097:1141	Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities.					
32071394	5	17	theme	phylogenetic	745:756	arg1	analyses					775:782	phylogenetic and phylogenomic analyses	745:782	phylogenetic and phylogenomic analyses	745:782	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	2	18	theme	Algal	227:231	arg1	polysaccharides					233:247	Algal polysaccharides	227:247	Algal polysaccharides	227:247	Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood.					
32071394	1	19	theme	organic	124:130	arg1	substrates					132:141	organic substrates	124:141	organic substrates	124:141	Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria.					
32071394	10	20	theme	degradation	1402:1412	arg1	genes					1414:1418	glycan degradation genes	1395:1418	glycan degradation genes	1395:1418	Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms.					
32071394	5	21	theme	phylogenomic	762:773	arg1	analyses					775:782	phylogenetic and phylogenomic analyses	745:782	phylogenetic and phylogenomic analyses	745:782	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	1	22	dep	Massive	104:110	arg1	releases					112:119	releases	112:119	releases	112:119	Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria.					
32071394	0	23	theme	North	73:77	arg1	blooms					96:101	North Sea spring algal blooms	73:101	North Sea spring algal blooms	73:101	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.					
32071394	7	24	theme	polysaccharide	1105:1118	arg1	capacities					1132:1141	limited polysaccharide utilization capacities	1097:1141	limited polysaccharide utilization capacities	1097:1141	Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities.					
32071394	1	25	theme	algal	157:161	arg1	blooms					163:168	marine algal blooms	150:168	marine algal blooms	150:168	Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria.					
32071394	0	26	theme	spring	83:88	arg1	blooms					96:101	North Sea spring algal blooms	73:101	North Sea spring algal blooms	73:101	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.					
32071394	7	27	theme	small	1078:1082	arg1	genomes					1084:1090	small genomes	1078:1090	small genomes with limited polysaccharide utilization capacities	1078:1141	Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities.					
32071394	10	28	theme	algae	1473:1477	arg1	blooms					1479:1484	Chattonella algae blooms	1461:1484	Chattonella algae blooms	1461:1484	Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms.					
32071394	5	29	theme	in	830:831	arg1	hybridization					838:850	fluorescence in situ hybridization	817:850	fluorescence in situ hybridization	817:850	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	0	30	theme	Polaribacter	46:57	arg1	clades					59:64	distinct Polaribacter clades	37:64	distinct Polaribacter clades	37:64	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.					
32071394	3	31	theme	utilization	452:462	arg1	capabilities					464:475	polysaccharide utilization capabilities	437:475	polysaccharide utilization capabilities	437:475	We investigated, whether polysaccharide utilization capabilities contribute to niche differentiation of Polaribacter spp.					
32071394	0	32	theme	Polysaccharide	0:13	arg1	partitioning					21:32	Polysaccharide niche partitioning	0:32	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.	0:102	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.					
32071394	9	33	contain	had	1309:1311	arg1	3-b					1276:1278	Polaribacter 3-b	1263:1278	Polaribacter 3-b	1263:1278	Polaribacter 3-b responded late in blooms and had the capacity to utilize sulfated xylan.					
32071394	9	33	contain	had	1309:1311	arg2	capacity					1317:1324	the capacity to utilize sulfated xylan	1313:1350	the capacity to utilize sulfated xylan	1313:1350	Polaribacter 3-b responded late in blooms and had the capacity to utilize sulfated xylan.					
32071394	8	34	theme	distinct	1204:1211	arg1	potential					1252:1260	a distinct sulfated α-glucoronomannan degradation potential	1202:1260	a distinct sulfated α-glucoronomannan degradation potential	1202:1260	Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential.					
32071394	7	35	theme	typical	1043:1049	arg1	responders					1057:1066	typical first responders	1043:1066	typical first responders featuring small genomes with limited polysaccharide utilization capacities	1043:1141	Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities.					
32071394	5	36	theme	gene	913:916	arg1	expression					918:927	in situ gene expression	905:927	in situ gene expression	905:927	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	8	37	theme	α-glucoronomannan	1222:1238	arg1	potential					1252:1260	a distinct sulfated α-glucoronomannan degradation potential	1202:1260	a distinct sulfated α-glucoronomannan degradation potential	1202:1260	Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential.					
32071394	6	38	theme	distinct	968:975	arg1	niches					992:997	distinct polysaccharide niches	968:997	distinct polysaccharide niches	968:997	Four clades with distinct polysaccharide niches were dominating.					
32071394	3	39	theme	niche	491:495	arg1	differentiation					497:511	niche differentiation	491:511	niche differentiation of Polaribacter spp	491:531	We investigated, whether polysaccharide utilization capabilities contribute to niche differentiation of Polaribacter spp.					
32071394	0	40	theme	distinct	37:44	arg1	clades					59:64	distinct Polaribacter clades	37:64	distinct Polaribacter clades	37:64	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.					
32071394	0	41	theme	clades	59:64	arg1	partitioning					21:32	Polysaccharide niche partitioning	0:32	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.	0:102	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.					
32071394	3	42	theme	spp	529:531	arg1	differentiation					497:511	niche differentiation	491:511	niche differentiation of Polaribacter spp	491:531	We investigated, whether polysaccharide utilization capabilities contribute to niche differentiation of Polaribacter spp.					
32071394	10	43	theme	Polaribacter	1353:1364	arg1	1-a					1366:1368	Polaribacter 1-a	1353:1368	Polaribacter 1-a	1353:1368	Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms.					
32071394	7	44	theme	Polaribacter	1016:1027	arg1	2-a					1029:1031	Polaribacter 2-a	1016:1031	Polaribacter 2-a	1016:1031	Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities.					
32071394	4	45	theme	southern	672:679	arg1	Sea					687:689	the southern North Sea	668:689	the southern North Sea	668:689	(class Flavobacteriia; known to include relevant polysaccharide-degraders) that were abundant during 2009-2012 spring algal blooms in the southern North Sea.					
32071394	11	46	theme	glycan	1575:1580	arg1	niches					1582:1587	distinct glycan niches	1566:1587	distinct glycan niches	1566:1587	These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.					
32071394	2	47	theme	community	368:376	arg1	composition					378:388	the microbial community composition	354:388	shaping the microbial community composition	346:388	Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood.					
32071394	11	48	theme	algal	1613:1617	arg1	blooms					1619:1624	North Sea spring algal blooms	1596:1624	North Sea spring algal blooms	1596:1624	These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.					
32071394	5	49	theme	Polaribacter	719:730	arg1	clades					732:737	six distinct Polaribacter clades	706:737	six distinct Polaribacter clades	706:737	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	5	50	dep	in	905:906	arg1	situ					908:911	situ	908:911	situ	908:911	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	4	51	theme	algal	652:656	arg1	blooms					658:663	2009-2012 spring algal blooms	635:663	2009-2012 spring algal blooms in the southern North Sea	635:689	(class Flavobacteriia; known to include relevant polysaccharide-degraders) that were abundant during 2009-2012 spring algal blooms in the southern North Sea.					
32071394	11	52	theme	Sea	1602:1604	arg1	blooms					1619:1624	North Sea spring algal blooms	1596:1624	North Sea spring algal blooms	1596:1624	These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.					
32071394	8	53	theme	3-a	1157:1159	arg1	Polaribacter					1144:1155	Polaribacter 3-a	1144:1159	Polaribacter 3-a	1144:1159	Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential.					
32071394	1	54	theme	many	188:191	arg1	clades					193:198	many clades	188:198	many clades of heterotrophic bacteria	188:224	Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria.					
32071394	1	55	theme	bacteria	217:224	arg1	clades					193:198	many clades	188:198	many clades of heterotrophic bacteria	188:224	Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria.					
32071394	11	56	theme	Polaribacter	1539:1550	arg1	clades					1552:1557	sympatric Polaribacter clades	1529:1557	sympatric Polaribacter clades	1529:1557	These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.					
32071394	5	57	dep	in	830:831	arg1	situ					833:836	situ	833:836	situ	833:836	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	10	58	theme	genes	1414:1418	arg1	numbers					1384:1390	high numbers	1379:1390	high numbers of glycan degradation genes	1379:1418	Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms.					
32071394	1	59	theme	substrates	132:141	arg1	Massive					104:110	Massive	104:110	Massive	104:110	Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria.					
32071394	10	60	theme	glycan	1395:1400	arg1	genes					1414:1418	glycan degradation genes	1395:1418	glycan degradation genes	1395:1418	Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms.					
32071394	0	61	theme	Sea	79:81	arg1	blooms					96:101	North Sea spring algal blooms	73:101	North Sea spring algal blooms	73:101	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.					
32071394	2	62	theme	diverse	268:274	arg1	class					301:305	the most diverse and structurally complex class	259:305	the most diverse and structurally complex class of these substrates	259:325	Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood.					
32071394	1	63	theme	marine	150:155	arg1	blooms					163:168	marine algal blooms	150:168	marine algal blooms	150:168	Massive releases of organic substrates during marine algal blooms trigger growth of many clades of heterotrophic bacteria.					
32071394	0	64	theme	algal	90:94	arg1	blooms					96:101	North Sea spring algal blooms	73:101	North Sea spring algal blooms	73:101	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.					
32071394	7	65	theme	utilization	1120:1130	arg1	capacities					1132:1141	limited polysaccharide utilization capacities	1097:1141	limited polysaccharide utilization capacities	1097:1141	Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities.					
32071394	4	66	from	blooms	658:663	arg1	Sea					687:689	the southern North Sea	668:689	the southern North Sea	668:689	(class Flavobacteriia; known to include relevant polysaccharide-degraders) that were abundant during 2009-2012 spring algal blooms in the southern North Sea.					
32071394	7	67	theme	limited	1097:1103	arg1	capacities					1132:1141	limited polysaccharide utilization capacities	1097:1141	limited polysaccharide utilization capacities	1097:1141	Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities.					
32071394	5	68	theme	fluorescence	817:828	arg1	hybridization					838:850	fluorescence in situ hybridization	817:850	fluorescence in situ hybridization	817:850	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	4	69	dep	abundant	619:626	arg1	Flavobacteriia					541:554	class Flavobacteriia	535:554	class Flavobacteriia; known to include relevant polysaccharide-degraders	535:606	(class Flavobacteriia; known to include relevant polysaccharide-degraders) that were abundant during 2009-2012 spring algal blooms in the southern North Sea.					
32071394	6	70	with	clades	956:961	arg1	niches					992:997	distinct polysaccharide niches	968:997	distinct polysaccharide niches	968:997	Four clades with distinct polysaccharide niches were dominating.					
32071394	4	71	theme	class	535:539	arg1	Flavobacteriia					541:554	class Flavobacteriia	535:554	class Flavobacteriia; known to include relevant polysaccharide-degraders	535:606	(class Flavobacteriia; known to include relevant polysaccharide-degraders) that were abundant during 2009-2012 spring algal blooms in the southern North Sea.					
32071394	10	72	theme	Chattonella	1461:1471	arg1	blooms					1479:1484	Chattonella algae blooms	1461:1484	Chattonella algae blooms	1461:1484	Polaribacter 1-a featured high numbers of glycan degradation genes and were particularly abundant following Chattonella algae blooms.					
32071394	0	73	theme	niche	15:19	arg1	partitioning					21:32	Polysaccharide niche partitioning	0:32	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.	0:102	Polysaccharide niche partitioning of distinct Polaribacter clades during North Sea spring algal blooms.					
32071394	9	74	theme	Polaribacter	1263:1274	arg1	3-b					1276:1278	Polaribacter 3-b	1263:1278	Polaribacter 3-b	1263:1278	Polaribacter 3-b responded late in blooms and had the capacity to utilize sulfated xylan.					
32071394	5	75	theme	in	905:906	arg1	expression					918:927	in situ gene expression	905:927	in situ gene expression	905:927	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	8	76	theme	degradation	1240:1250	arg1	potential					1252:1260	a distinct sulfated α-glucoronomannan degradation potential	1202:1260	a distinct sulfated α-glucoronomannan degradation potential	1202:1260	Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential.					
32071394	7	77	theme	first	1051:1055	arg1	responders					1057:1066	typical first responders	1043:1066	typical first responders featuring small genomes with limited polysaccharide utilization capacities	1043:1141	Polaribacter 2-a comprised typical first responders featuring small genomes with limited polysaccharide utilization capacities.					
32071394	2	78	dep	shaping	346:352	arg1	composition					378:388	the microbial community composition	354:388	shaping the microbial community composition	346:388	Algal polysaccharides represent the most diverse and structurally complex class of these substrates, yet their role in shaping the microbial community composition is poorly understood.					
32071394	3	79	theme	Polaribacter	516:527	arg1	spp					529:531	Polaribacter spp	516:531	Polaribacter spp	516:531	We investigated, whether polysaccharide utilization capabilities contribute to niche differentiation of Polaribacter spp.					
32071394	5	80	theme	metagenome-assembled	862:881	arg1	genomes					883:889	metagenome-assembled genomes	862:889	metagenome-assembled genomes	862:889	We identified six distinct Polaribacter clades using phylogenetic and phylogenomic analyses, quantified their abundances via fluorescence in situ hybridization, compared metagenome-assembled genomes, and assessed in situ gene expression using metaproteomics.					
32071394	3	81	theme	polysaccharide	437:450	arg1	capabilities					464:475	polysaccharide utilization capabilities	437:475	polysaccharide utilization capabilities	437:475	We investigated, whether polysaccharide utilization capabilities contribute to niche differentiation of Polaribacter spp.					
32071394	4	82	theme	relevant	574:581	arg1	polysaccharide-degraders					583:606	relevant polysaccharide-degraders	574:606	relevant polysaccharide-degraders	574:606	(class Flavobacteriia; known to include relevant polysaccharide-degraders) that were abundant during 2009-2012 spring algal blooms in the southern North Sea.					
32071394	11	83	theme	North	1596:1600	arg1	blooms					1619:1624	North Sea spring algal blooms	1596:1624	North Sea spring algal blooms	1596:1624	These results support the hypothesis that sympatric Polaribacter clades occupy distinct glycan niches during North Sea spring algal blooms.					
32071394	8	84	from	abundant	1166:1173	arg1	2010					1183:1186	2010	1183:1186	2010	1183:1186	Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential.					
32071394	4	85	theme	North	681:685	arg1	Sea					687:689	the southern North Sea	668:689	the southern North Sea	668:689	(class Flavobacteriia; known to include relevant polysaccharide-degraders) that were abundant during 2009-2012 spring algal blooms in the southern North Sea.					
32071394	8	86	dep	distinct	1204:1211	arg1	sulfated					1213:1220	sulfated	1213:1220	sulfated	1213:1220	Polaribacter 3-a were abundant only in 2010 and possessed a distinct sulfated α-glucoronomannan degradation potential.					
34968546	0	0	theme	fucans	79:84	arg1	preparation					43:53	preparation	43:53	preparation of high purity sulfated fucans	43:84	Systematic comparison of eight methods for preparation of high purity sulfated fucans extracted from the brown alga Pelvetia canaliculata.					
34968546	7	1	theme	bacterium	1093:1101	arg1	fucanivorans					1118:1129	the marine bacterium Mariniflexille fucanivorans	1082:1129	the marine bacterium Mariniflexille fucanivorans	1082:1129	Mass spectrometry analyses were done on oligosaccharides produced by the fucanase MfFcnA from the marine bacterium Mariniflexille fucanivorans.					
34968546	6	2	theme	Acidic	834:839	arg1	extractions					841:851	Acidic extractions	834:851	Acidic extractions	834:851	Acidic extractions provided higher yields compared to neutral ones, whereas the AC purification provided sulfated fucan products with the highest purity.					
34968546	4	3	theme	fucan	540:544	arg1	methods					558:564	Sulfated fucan preparation methods	531:564	Sulfated fucan preparation methods	531:564	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	0	4	theme	sulfated	70:77	arg1	fucans					79:84	high purity sulfated fucans	58:84	high purity sulfated fucans	58:84	Systematic comparison of eight methods for preparation of high purity sulfated fucans extracted from the brown alga Pelvetia canaliculata.					
34968546	7	5	theme	Mariniflexille	1103:1116	arg1	fucanivorans					1118:1129	the marine bacterium Mariniflexille fucanivorans	1082:1129	the marine bacterium Mariniflexille fucanivorans	1082:1129	Mass spectrometry analyses were done on oligosaccharides produced by the fucanase MfFcnA from the marine bacterium Mariniflexille fucanivorans.					
34968546	2	6	theme	growing	332:338	arg1	need					340:343	a growing need	330:343	a growing need to develop reliable and cost effective protocols for their preparation	330:414	To learn more on their structure and to analyze and exploit their biological activities, there is a growing need to develop reliable and cost effective protocols for their preparation.					
34968546	4	7	theme	Sulfated	531:538	arg1	fucan					540:544	Sulfated fucan	531:544	Sulfated fucan preparation methods	531:564	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	8	8	theme	unique	1150:1155	arg1	insight					1157:1163	unique insight	1150:1163	unique insight into enzyme specificity	1150:1187	This has provided unique insight into enzyme specificity and the structural characteristics of sulfated fucans.					
34968546	3	9	theme	rich	499:502	arg1	canaliculata					461:472	a brown alga Pelvetia canaliculata	439:472	a brown alga Pelvetia canaliculata (Linnaeus)	439:483	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	9	theme	rich	499:502	arg1	source					504:509	a rich source	497:509	a rich source of sulfated fucans	497:528	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	10	theme	sulfated	514:521	arg1	fucans					523:528	sulfated fucans	514:528	sulfated fucans	514:528	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	4	11	theme	cetylpyridinium	697:711	arg1	chloride					713:720	cetylpyridinium chloride	697:720	cetylpyridinium chloride (CPC)	697:726	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	4	11	theme	cetylpyridinium	697:711	arg1	CPC					723:725	CPC	723:725	CPC	723:725	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	6	12	theme	higher	862:867	arg1	yields					869:874	higher yields	862:874	higher yields	862:874	Acidic extractions provided higher yields compared to neutral ones, whereas the AC purification provided sulfated fucan products with the highest purity.					
34968546	7	13	theme	marine	1086:1091	arg1	fucanivorans					1118:1129	the marine bacterium Mariniflexille fucanivorans	1082:1129	the marine bacterium Mariniflexille fucanivorans	1082:1129	Mass spectrometry analyses were done on oligosaccharides produced by the fucanase MfFcnA from the marine bacterium Mariniflexille fucanivorans.					
34968546	4	14	theme	preparation	546:556	arg1	methods					558:564	Sulfated fucan preparation methods	531:564	Sulfated fucan preparation methods	531:564	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	0	15	theme	brown	105:109	arg1	canaliculata					125:136	the brown alga Pelvetia canaliculata	101:136	the brown alga Pelvetia canaliculata	101:136	Systematic comparison of eight methods for preparation of high purity sulfated fucans extracted from the brown alga Pelvetia canaliculata.					
34968546	2	16	theme	effective	374:382	arg1	protocols					384:392	reliable and cost effective protocols	356:392	reliable and cost effective protocols for their preparation	356:414	To learn more on their structure and to analyze and exploit their biological activities, there is a growing need to develop reliable and cost effective protocols for their preparation.					
34968546	6	17	theme	fucan	948:952	arg1	products					954:961	sulfated fucan products	939:961	sulfated fucan products with the highest purity	939:985	Acidic extractions provided higher yields compared to neutral ones, whereas the AC purification provided sulfated fucan products with the highest purity.					
34968546	8	18	theme	enzyme	1170:1175	arg1	specificity					1177:1187	enzyme specificity	1170:1187	enzyme specificity	1170:1187	This has provided unique insight into enzyme specificity and the structural characteristics of sulfated fucans.					
34968546	3	19	theme	present	424:430	arg1	study					432:436	the present study	420:436	the present study	420:436	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	5	20	theme	Final	729:733	arg1	products					735:742	Final products	729:742	Final products	729:742	Final products were compared in terms of yield, purity, monosaccharide composition and molecular weight.					
34968546	3	21	theme	fucans	523:528	arg1	canaliculata					461:472	a brown alga Pelvetia canaliculata	439:472	a brown alga Pelvetia canaliculata (Linnaeus)	439:483	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	21	theme	fucans	523:528	arg1	source					504:509	a rich source	497:509	a rich source of sulfated fucans	497:528	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	5	22	theme	weight	826:831	arg1	terms					761:765	terms	761:765	terms of yield, purity, monosaccharide composition and molecular weight	761:831	Final products were compared in terms of yield, purity, monosaccharide composition and molecular weight.					
34968546	0	23	theme	Systematic	0:9	arg1	comparison					11:20	Systematic comparison	0:20	Systematic comparison of eight methods for preparation of high purity sulfated fucans	0:84	Systematic comparison of eight methods for preparation of high purity sulfated fucans extracted from the brown alga Pelvetia canaliculata.					
34968546	6	24	theme	sulfated	939:946	arg1	products					954:961	sulfated fucan products	939:961	sulfated fucan products with the highest purity	939:985	Acidic extractions provided higher yields compared to neutral ones, whereas the AC purification provided sulfated fucan products with the highest purity.					
34968546	7	25	theme	fucanase	1061:1068	arg1	MfFcnA					1070:1075	the fucanase MfFcnA	1057:1075	the fucanase MfFcnA from the marine bacterium Mariniflexille fucanivorans	1057:1129	Mass spectrometry analyses were done on oligosaccharides produced by the fucanase MfFcnA from the marine bacterium Mariniflexille fucanivorans.					
34968546	4	26	theme	activated	636:644	arg1	AC					656:657	AC	656:657	AC	656:657	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	4	26	theme	activated	636:644	arg1	charcoal					646:653	activated charcoal	636:653	activated charcoal (AC)	636:658	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	2	27	theme	biological	298:307	arg1	activities					309:318	their biological activities	292:318	their biological activities	292:318	To learn more on their structure and to analyze and exploit their biological activities, there is a growing need to develop reliable and cost effective protocols for their preparation.					
34968546	2	28	theme	reliable	356:363	arg1	protocols					384:392	reliable and cost effective protocols	356:392	reliable and cost effective protocols for their preparation	356:414	To learn more on their structure and to analyze and exploit their biological activities, there is a growing need to develop reliable and cost effective protocols for their preparation.					
34968546	5	29	theme	yield	770:774	arg1	terms					761:765	terms	761:765	terms of yield, purity, monosaccharide composition and molecular weight	761:831	Final products were compared in terms of yield, purity, monosaccharide composition and molecular weight.					
34968546	0	30	theme	Pelvetia	116:123	arg1	canaliculata					125:136	the brown alga Pelvetia canaliculata	101:136	the brown alga Pelvetia canaliculata	101:136	Systematic comparison of eight methods for preparation of high purity sulfated fucans extracted from the brown alga Pelvetia canaliculata.					
34968546	5	31	theme	purity	777:782	arg1	terms					761:765	terms	761:765	terms of yield, purity, monosaccharide composition and molecular weight	761:831	Final products were compared in terms of yield, purity, monosaccharide composition and molecular weight.					
34968546	0	32	theme	alga	111:114	arg1	canaliculata					125:136	the brown alga Pelvetia canaliculata	101:136	the brown alga Pelvetia canaliculata	101:136	Systematic comparison of eight methods for preparation of high purity sulfated fucans extracted from the brown alga Pelvetia canaliculata.					
34968546	1	33	theme	brown	160:164	arg1	algae					166:170	brown algae	160:170	brown algae	160:170	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	3	34	theme	brown	441:445	arg1	canaliculata					461:472	a brown alga Pelvetia canaliculata	439:472	a brown alga Pelvetia canaliculata (Linnaeus)	439:483	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	34	theme	brown	441:445	arg1	source					504:509	a rich source	497:509	a rich source of sulfated fucans	497:528	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	34	theme	brown	441:445	arg1	Linnaeus					475:482	Linnaeus	475:482	Linnaeus	475:482	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	6	35	theme	AC	914:915	arg1	purification					917:928	the AC purification	910:928	the AC purification	910:928	Acidic extractions provided higher yields compared to neutral ones, whereas the AC purification provided sulfated fucan products with the highest purity.					
34968546	1	36	theme	heterogeneous	178:190	arg1	fucans					148:153	Sulfated fucans	139:153	Sulfated fucans from brown algae	139:170	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	1	36	theme	heterogeneous	178:190	arg1	molecules					221:229	biologically active molecules	201:229	biologically active molecules	201:229	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	1	36	theme	heterogeneous	178:190	arg1	group					192:196	a heterogeneous group	176:196	a heterogeneous group of biologically active molecules	176:229	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	5	37	theme	monosaccharide	785:798	arg1	composition					800:810	monosaccharide composition	785:810	monosaccharide composition	785:810	Final products were compared in terms of yield, purity, monosaccharide composition and molecular weight.					
34968546	0	38	theme	methods	31:37	arg1	comparison					11:20	Systematic comparison	0:20	Systematic comparison of eight methods for preparation of high purity sulfated fucans	0:84	Systematic comparison of eight methods for preparation of high purity sulfated fucans extracted from the brown alga Pelvetia canaliculata.					
34968546	8	39	theme	structural	1197:1206	arg1	characteristics					1208:1222	the structural characteristics	1193:1222	the structural characteristics of sulfated fucans	1193:1241	This has provided unique insight into enzyme specificity and the structural characteristics of sulfated fucans.					
34968546	1	40	theme	active	214:219	arg1	molecules					221:229	biologically active molecules	201:229	biologically active molecules	201:229	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	8	41	theme	sulfated	1227:1234	arg1	fucans					1236:1241	sulfated fucans	1227:1241	sulfated fucans	1227:1241	This has provided unique insight into enzyme specificity and the structural characteristics of sulfated fucans.					
34968546	7	42	theme	Mass	988:991	arg1	analyses					1006:1013	Mass spectrometry analyses	988:1013	Mass spectrometry analyses	988:1013	Mass spectrometry analyses were done on oligosaccharides produced by the fucanase MfFcnA from the marine bacterium Mariniflexille fucanivorans.					
34968546	7	43	from	fucanivorans	1118:1129	arg1	MfFcnA					1070:1075	the fucanase MfFcnA	1057:1075	the fucanase MfFcnA from the marine bacterium Mariniflexille fucanivorans	1057:1129	Mass spectrometry analyses were done on oligosaccharides produced by the fucanase MfFcnA from the marine bacterium Mariniflexille fucanivorans.					
34968546	4	44	with	purification	618:629	arg1	PVPP					687:690	PVPP	687:690	PVPP	687:690	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	4	44	with	purification	618:629	arg1	CPC					723:725	CPC	723:725	CPC	723:725	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	4	44	with	purification	618:629	arg1	AC					656:657	AC	656:657	AC	656:657	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	4	44	with	purification	618:629	arg1	polyvinylpolypyrrolidone					661:684	polyvinylpolypyrrolidone	661:684	polyvinylpolypyrrolidone (PVPP)	661:691	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	4	44	with	purification	618:629	arg1	chloride					713:720	cetylpyridinium chloride	697:720	cetylpyridinium chloride (CPC)	697:726	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	4	44	with	purification	618:629	arg1	charcoal					646:653	activated charcoal	636:653	activated charcoal (AC)	636:658	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	7	45	theme	spectrometry	993:1004	arg1	analyses					1006:1013	Mass spectrometry analyses	988:1013	Mass spectrometry analyses	988:1013	Mass spectrometry analyses were done on oligosaccharides produced by the fucanase MfFcnA from the marine bacterium Mariniflexille fucanivorans.					
34968546	6	46	with	products	954:961	arg1	purity					980:985	the highest purity	968:985	the highest purity	968:985	Acidic extractions provided higher yields compared to neutral ones, whereas the AC purification provided sulfated fucan products with the highest purity.					
34968546	3	47	theme	alga	447:450	arg1	canaliculata					461:472	a brown alga Pelvetia canaliculata	439:472	a brown alga Pelvetia canaliculata (Linnaeus)	439:483	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	47	theme	alga	447:450	arg1	source					504:509	a rich source	497:509	a rich source of sulfated fucans	497:528	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	47	theme	alga	447:450	arg1	Linnaeus					475:482	Linnaeus	475:482	Linnaeus	475:482	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	1	48	theme	molecules	221:229	arg1	fucans					148:153	Sulfated fucans	139:153	Sulfated fucans from brown algae	139:170	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	1	48	theme	molecules	221:229	arg1	molecules					221:229	biologically active molecules	201:229	biologically active molecules	201:229	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	1	48	theme	molecules	221:229	arg1	group					192:196	a heterogeneous group	176:196	a heterogeneous group of biologically active molecules	176:229	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	8	49	theme	fucans	1236:1241	arg1	characteristics					1208:1222	the structural characteristics	1193:1222	the structural characteristics of sulfated fucans	1193:1241	This has provided unique insight into enzyme specificity and the structural characteristics of sulfated fucans.					
34968546	8	49	theme	fucans	1236:1241	arg1	insight					1157:1163	unique insight	1150:1163	unique insight into enzyme specificity	1150:1187	This has provided unique insight into enzyme specificity and the structural characteristics of sulfated fucans.					
34968546	3	50	theme	Pelvetia	452:459	arg1	canaliculata					461:472	a brown alga Pelvetia canaliculata	439:472	a brown alga Pelvetia canaliculata (Linnaeus)	439:483	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	50	theme	Pelvetia	452:459	arg1	source					504:509	a rich source	497:509	a rich source of sulfated fucans	497:528	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	50	theme	Pelvetia	452:459	arg1	Linnaeus					475:482	Linnaeus	475:482	Linnaeus	475:482	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	0	51	theme	purity	63:68	arg1	fucans					79:84	high purity sulfated fucans	58:84	high purity sulfated fucans	58:84	Systematic comparison of eight methods for preparation of high purity sulfated fucans extracted from the brown alga Pelvetia canaliculata.					
34968546	5	52	theme	composition	800:810	arg1	terms					761:765	terms	761:765	terms of yield, purity, monosaccharide composition and molecular weight	761:831	Final products were compared in terms of yield, purity, monosaccharide composition and molecular weight.					
34968546	3	53	used	used	489:492	arg2	source					504:509	a rich source	497:509	a rich source of sulfated fucans	497:528	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	53	used	used	489:492	arg2	Linnaeus					475:482	Linnaeus	475:482	Linnaeus	475:482	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	3	53	used	used	489:492	arg2	canaliculata					461:472	a brown alga Pelvetia canaliculata	439:472	a brown alga Pelvetia canaliculata (Linnaeus)	439:483	In the present study, a brown alga Pelvetia canaliculata (Linnaeus) was used as a rich source of sulfated fucans.					
34968546	1	54	theme	Sulfated	139:146	arg1	fucans					148:153	Sulfated fucans	139:153	Sulfated fucans from brown algae	139:170	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	1	54	theme	Sulfated	139:146	arg1	molecules					221:229	biologically active molecules	201:229	biologically active molecules	201:229	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	1	54	theme	Sulfated	139:146	arg1	group					192:196	a heterogeneous group	176:196	a heterogeneous group of biologically active molecules	176:229	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	5	55	theme	molecular	816:824	arg1	weight					826:831	molecular weight	816:831	molecular weight	816:831	Final products were compared in terms of yield, purity, monosaccharide composition and molecular weight.					
34968546	4	56	theme	acidic	587:592	arg1	extractions					594:604	neutral and acidic extractions	575:604	neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC)	575:726	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	0	57	theme	high	58:61	arg1	fucans					79:84	high purity sulfated fucans	58:84	high purity sulfated fucans	58:84	Systematic comparison of eight methods for preparation of high purity sulfated fucans extracted from the brown alga Pelvetia canaliculata.					
34968546	6	58	theme	neutral	888:894	arg1	ones					896:899	neutral ones	888:899	neutral ones	888:899	Acidic extractions provided higher yields compared to neutral ones, whereas the AC purification provided sulfated fucan products with the highest purity.					
34968546	6	59	dep	provided	853:860	arg1	whereas					902:908	whereas	902:908	whereas	902:908	Acidic extractions provided higher yields compared to neutral ones, whereas the AC purification provided sulfated fucan products with the highest purity.					
34968546	1	60	from	algae	166:170	arg1	fucans					148:153	Sulfated fucans	139:153	Sulfated fucans from brown algae	139:170	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	1	60	from	algae	166:170	arg1	molecules					221:229	biologically active molecules	201:229	biologically active molecules	201:229	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	1	60	from	algae	166:170	arg1	group					192:196	a heterogeneous group	176:196	a heterogeneous group of biologically active molecules	176:229	Sulfated fucans from brown algae are a heterogeneous group of biologically active molecules.					
34968546	4	61	theme	neutral	575:581	arg1	extractions					594:604	neutral and acidic extractions	575:604	neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC)	575:726	Sulfated fucan preparation methods included neutral and acidic extractions followed by purification with activated charcoal (AC), polyvinylpolypyrrolidone (PVPP), or cetylpyridinium chloride (CPC).					
34968546	6	62	theme	highest	972:978	arg1	purity					980:985	the highest purity	968:985	the highest purity	968:985	Acidic extractions provided higher yields compared to neutral ones, whereas the AC purification provided sulfated fucan products with the highest purity.					
34355888	8	0	theme	animal	1122:1127	arg1	models					1129:1134	two animal models	1118:1134	two animal models	1118:1134	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	8	0	theme	animal	1122:1127	arg1	model					1237:1241	the burn injury mouse model	1215:1241	the burn injury mouse model	1215:1241	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	8	0	theme	animal	1122:1127	arg1	model					1205:1209	the acetaminophen (APAP)-induced acute liver injury mouse model	1147:1209	the acetaminophen (APAP)-induced acute liver injury mouse model	1147:1209	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	5	1	theme	2-diethylamino	628:641	arg1	AMQC					673:676	AMQC	673:676	AMQC	673:676	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	5	1	theme	2-diethylamino	628:641	arg1	reagent					578:584	a new labeling reagent	563:584	a new labeling reagent for HS disaccharides analysis	563:614	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	5	1	theme	2-diethylamino	628:641	arg1	quinoline-2-carboamide					649:670	6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide	617:670	6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC)	617:677	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	9	2	theme	new	1343:1345	arg1	set					1347:1349	a potential new set	1331:1349	a potential new set of biomarkers for disease diagnosis and prognosis	1331:1399	The method may uncover the roles of HS contributing to the diseases as well as provide a potential new set of biomarkers for disease diagnosis and prognosis.					
34355888	9	2	theme	new	1343:1345	arg1	biomarkers					1354:1363	biomarkers	1354:1363	biomarkers for disease diagnosis and prognosis	1354:1399	The method may uncover the roles of HS contributing to the diseases as well as provide a potential new set of biomarkers for disease diagnosis and prognosis.					
34355888	1	3	theme	animal	142:147	arg1	kingdom					149:155	the animal kingdom	138:155	the animal kingdom	138:155	Heparan sulfates (HSs) are widely expressed glycans in the animal kingdom.					
34355888	9	4	theme	biomarkers	1354:1363	arg1	set					1347:1349	a potential new set	1331:1349	a potential new set of biomarkers for disease diagnosis and prognosis	1331:1399	The method may uncover the roles of HS contributing to the diseases as well as provide a potential new set of biomarkers for disease diagnosis and prognosis.					
34355888	9	4	theme	biomarkers	1354:1363	arg1	biomarkers					1354:1363	biomarkers	1354:1363	biomarkers for disease diagnosis and prognosis	1354:1399	The method may uncover the roles of HS contributing to the diseases as well as provide a potential new set of biomarkers for disease diagnosis and prognosis.					
34355888	4	5	theme	biological	506:515	arg1	samples					517:523	biological samples	506:523	biological samples	506:523	A sensitive and reliable quantitative technique for the analysis of HS from biological samples is under development.					
34355888	8	6	theme	mouse	1199:1203	arg1	model					1205:1209	the acetaminophen (APAP)-induced acute liver injury mouse model	1147:1209	the acetaminophen (APAP)-induced acute liver injury mouse model	1147:1209	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	3	7	theme	information	346:356	arg1	HS					324:325	HS	324:325	HS	324:325	The amount of HS and its structural information are critically important for investigating the functions of HS in vivo.					
34355888	3	7	theme	information	346:356	arg1	amount					314:319	The amount	310:319	The amount of HS and its structural information	310:356	The amount of HS and its structural information are critically important for investigating the functions of HS in vivo.					
34355888	3	7	theme	information	346:356	arg1	information					346:356	its structural information	331:356	its structural information	331:356	The amount of HS and its structural information are critically important for investigating the functions of HS in vivo.					
34355888	3	7	theme	information	346:356	arg1	important					373:381	important	373:381	important	373:381	The amount of HS and its structural information are critically important for investigating the functions of HS in vivo.					
34355888	5	8	theme	ethyl	643:647	arg1	AMQC					673:676	AMQC	673:676	AMQC	673:676	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	5	8	theme	ethyl	643:647	arg1	reagent					578:584	a new labeling reagent	563:584	a new labeling reagent for HS disaccharides analysis	563:614	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	5	8	theme	ethyl	643:647	arg1	quinoline-2-carboamide					649:670	6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide	617:670	6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC)	617:677	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	7	9	theme	HS	961:962	arg1	amount					914:919	amount	914:919	amount	914:919	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	7	9	theme	HS	961:962	arg1	composition					942:952	the disaccharide composition	925:952	the disaccharide composition	925:952	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	7	9	theme	HS	961:962	arg1	HS					961:962	the HS	957:962	the HS	957:962	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	9	10	theme	disease	1369:1375	arg1	diagnosis					1377:1385	disease diagnosis	1369:1385	disease diagnosis	1369:1385	The method may uncover the roles of HS contributing to the diseases as well as provide a potential new set of biomarkers for disease diagnosis and prognosis.					
34355888	5	11	theme	HS	590:591	arg1	analysis					607:614	HS disaccharides analysis	590:614	HS disaccharides analysis	590:614	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	8	12	dep	plasma/serum	1051:1062	arg1	the					1047:1049	the	1047:1049	the	1047:1049	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	7	13	theme	internal	865:872	arg1	standards					874:882	authentic 13C-labeled HS disaccharide internal standards	827:882	authentic 13C-labeled HS disaccharide internal standards	827:882	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	4	14	from	analysis	486:493	arg1	samples					517:523	biological samples	506:523	biological samples	506:523	A sensitive and reliable quantitative technique for the analysis of HS from biological samples is under development.					
34355888	5	15	theme	disaccharides	593:605	arg1	analysis					607:614	HS disaccharides analysis	590:614	HS disaccharides analysis	590:614	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	7	16	theme	AMQC	809:812	arg1	use					802:804	The use	798:804	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards	798:882	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	3	17	theme	structural	335:344	arg1	information					346:356	its structural information	331:356	its structural information	331:356	The amount of HS and its structural information are critically important for investigating the functions of HS in vivo.					
34355888	9	18	theme	HS	1280:1281	arg1	roles					1271:1275	the roles	1267:1275	the roles of HS contributing to the diseases	1267:1310	The method may uncover the roles of HS contributing to the diseases as well as provide a potential new set of biomarkers for disease diagnosis and prognosis.					
34355888	8	19	theme	injury	1192:1197	arg1	model					1205:1209	the acetaminophen (APAP)-induced acute liver injury mouse model	1147:1209	the acetaminophen (APAP)-induced acute liver injury mouse model	1147:1209	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	1	20	theme	Heparan	83:89	arg1	glycans					127:133	widely expressed glycans	110:133	widely expressed glycans	110:133	Heparan sulfates (HSs) are widely expressed glycans in the animal kingdom.					
34355888	1	20	theme	Heparan	83:89	arg1	HSs					101:103	HSs	101:103	HSs	101:103	Heparan sulfates (HSs) are widely expressed glycans in the animal kingdom.					
34355888	1	20	theme	Heparan	83:89	arg1	sulfates					91:98	Heparan sulfates	83:98	Heparan sulfates (HSs)	83:104	Heparan sulfates (HSs) are widely expressed glycans in the animal kingdom.					
34355888	6	21	theme	AMQC-conjugated	684:698	arg1	disaccharides					700:712	The AMQC-conjugated disaccharides	680:712	The AMQC-conjugated disaccharides	680:712	The AMQC-conjugated disaccharides are analyzed by LC-MS/MS in positive mode, significantly improving the sensitivity.					
34355888	7	22	theme	single	969:974	arg1	slide					989:993	a single histological slide	967:993	a single histological slide	967:993	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	8	23	from	profile	1019:1025	arg1	tissues/organs					1068:1081	tissues/organs	1068:1081	tissues/organs	1068:1081	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	8	23	from	profile	1019:1025	arg1	plasma/serum					1051:1062	plasma/serum	1051:1062	plasma/serum	1051:1062	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	5	24	theme	6-amino-N-	617:626	arg1	AMQC					673:676	AMQC	673:676	AMQC	673:676	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	5	24	theme	6-amino-N-	617:626	arg1	reagent					578:584	a new labeling reagent	563:584	a new labeling reagent for HS disaccharides analysis	563:614	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	5	24	theme	6-amino-N-	617:626	arg1	quinoline-2-carboamide					649:670	6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide	617:670	6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC)	617:677	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	1	25	from	glycans	127:133	arg1	kingdom					149:155	the animal kingdom	138:155	the animal kingdom	138:155	Heparan sulfates (HSs) are widely expressed glycans in the animal kingdom.					
34355888	7	26	theme	disaccharide	852:863	arg1	standards					874:882	authentic 13C-labeled HS disaccharide internal standards	827:882	authentic 13C-labeled HS disaccharide internal standards	827:882	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	8	27	theme	injury	1224:1229	arg1	model					1237:1241	the burn injury mouse model	1215:1241	the burn injury mouse model	1215:1241	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	5	28	theme	new	565:567	arg1	reagent					578:584	a new labeling reagent	563:584	a new labeling reagent for HS disaccharides analysis	563:614	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	5	28	theme	new	565:567	arg1	quinoline-2-carboamide					649:670	6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide	617:670	6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC)	617:677	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	7	29	from	amount	914:919	arg1	slide					989:993	a single histological slide	967:993	a single histological slide	967:993	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	7	30	theme	authentic	827:835	arg1	standards					874:882	authentic 13C-labeled HS disaccharide internal standards	827:882	authentic 13C-labeled HS disaccharide internal standards	827:882	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	8	31	theme	burn	1219:1222	arg1	model					1237:1241	the burn injury mouse model	1215:1241	the burn injury mouse model	1215:1241	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	8	32	theme	acute	1180:1184	arg1	injury					1192:1197	the acetaminophen (APAP)-induced acute liver injury	1147:1197	the acetaminophen (APAP)-induced acute liver injury mouse model	1147:1209	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	7	33	theme	disaccharide	929:940	arg1	composition					942:952	the disaccharide composition	925:952	the disaccharide composition	925:952	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	5	34	theme	labeling	569:576	arg1	reagent					578:584	a new labeling reagent	563:584	a new labeling reagent for HS disaccharides analysis	563:614	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	5	34	theme	labeling	569:576	arg1	quinoline-2-carboamide					649:670	6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide	617:670	6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC)	617:677	Here, we report a new labeling reagent for HS disaccharides analysis, 6-amino-N-(2-diethylamino)ethyl quinoline-2-carboamide (AMQC).					
34355888	7	35	theme	13C-labeled	837:847	arg1	standards					874:882	authentic 13C-labeled HS disaccharide internal standards	827:882	authentic 13C-labeled HS disaccharide internal standards	827:882	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	8	36	theme	-induced	1171:1178	arg1	injury					1192:1197	the acetaminophen (APAP)-induced acute liver injury	1147:1197	the acetaminophen (APAP)-induced acute liver injury mouse model	1147:1209	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	2	37	theme	viral	293:297	arg1	infection					299:307	viral infection	293:307	viral infection	293:307	HS plays a role in regulating cell differentiation/proliferation, embryonic development, blood coagulation, inflammatory response, and viral infection.					
34355888	8	38	used	used	999:1002	arg2	We					996:997	We	996:997	We	996:997	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	8	39	theme	mouse	1231:1235	arg1	model					1237:1241	the burn injury mouse model	1215:1241	the burn injury mouse model	1215:1241	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	0	40	theme	Heparan	42:48	arg1	Sulfate					50:56	Heparan Sulfate	42:56	Heparan Sulfate from Biological Samples	42:80	Improving the Sensitivity for Quantifying Heparan Sulfate from Biological Samples.					
34355888	7	41	from	composition	942:952	arg1	slide					989:993	a single histological slide	967:993	a single histological slide	967:993	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	4	42	theme	sensitive	432:440	arg1	technique					468:476	A sensitive and reliable quantitative technique	430:476	A sensitive and reliable quantitative technique for the analysis of HS from biological samples	430:523	A sensitive and reliable quantitative technique for the analysis of HS from biological samples is under development.					
34355888	3	43	theme	HS	418:419	arg1	functions					405:413	the functions	401:413	the functions of HS	401:419	The amount of HS and its structural information are critically important for investigating the functions of HS in vivo.					
34355888	8	44	theme	liver	1186:1190	arg1	injury					1192:1197	the acetaminophen (APAP)-induced acute liver injury	1147:1197	the acetaminophen (APAP)-induced acute liver injury mouse model	1147:1209	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	4	45	theme	quantitative	455:466	arg1	technique					468:476	A sensitive and reliable quantitative technique	430:476	A sensitive and reliable quantitative technique for the analysis of HS from biological samples	430:523	A sensitive and reliable quantitative technique for the analysis of HS from biological samples is under development.					
34355888	2	46	theme	embryonic	224:232	arg1	development					234:244	embryonic development	224:244	embryonic development	224:244	HS plays a role in regulating cell differentiation/proliferation, embryonic development, blood coagulation, inflammatory response, and viral infection.					
34355888	8	47	theme	disease	1097:1103	arg1	prognosis					1105:1113	the disease prognosis	1093:1113	the disease prognosis	1093:1113	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	4	48	theme	reliable	446:453	arg1	technique					468:476	A sensitive and reliable quantitative technique	430:476	A sensitive and reliable quantitative technique for the analysis of HS from biological samples	430:523	A sensitive and reliable quantitative technique for the analysis of HS from biological samples is under development.					
34355888	4	49	from	samples	517:523	arg1	HS					498:499	HS	498:499	HS from biological samples	498:523	A sensitive and reliable quantitative technique for the analysis of HS from biological samples is under development.					
34355888	4	49	from	samples	517:523	arg1	analysis					486:493	the analysis	482:493	the analysis of HS from biological samples	482:523	A sensitive and reliable quantitative technique for the analysis of HS from biological samples is under development.					
34355888	2	50	theme	cell	188:191	arg1	differentiation/proliferation					193:221	cell differentiation/proliferation	188:221	cell differentiation/proliferation	188:221	HS plays a role in regulating cell differentiation/proliferation, embryonic development, blood coagulation, inflammatory response, and viral infection.					
34355888	8	51	theme	HS	1041:1042	arg1	levels					1031:1036	the levels	1027:1036	the levels of HS	1027:1042	We used this method to profile the levels of HS in the plasma/serum and tissues/organs to assist the disease prognosis in two animal models, including the acetaminophen (APAP)-induced acute liver injury mouse model and the burn injury mouse model.					
34355888	0	52	from	Samples	74:80	arg1	Sulfate					50:56	Heparan Sulfate	42:56	Heparan Sulfate from Biological Samples	42:80	Improving the Sensitivity for Quantifying Heparan Sulfate from Biological Samples.					
34355888	6	53	theme	positive	742:749	arg1	mode					751:754	positive mode	742:754	positive mode	742:754	The AMQC-conjugated disaccharides are analyzed by LC-MS/MS in positive mode, significantly improving the sensitivity.					
34355888	1	54	theme	expressed	117:125	arg1	glycans					127:133	widely expressed glycans	110:133	widely expressed glycans	110:133	Heparan sulfates (HSs) are widely expressed glycans in the animal kingdom.					
34355888	1	54	theme	expressed	117:125	arg1	sulfates					91:98	Heparan sulfates	83:98	Heparan sulfates (HSs)	83:104	Heparan sulfates (HSs) are widely expressed glycans in the animal kingdom.					
34355888	9	55	theme	potential	1333:1341	arg1	set					1347:1349	a potential new set	1331:1349	a potential new set of biomarkers for disease diagnosis and prognosis	1331:1399	The method may uncover the roles of HS contributing to the diseases as well as provide a potential new set of biomarkers for disease diagnosis and prognosis.					
34355888	9	55	theme	potential	1333:1341	arg1	biomarkers					1354:1363	biomarkers	1354:1363	biomarkers for disease diagnosis and prognosis	1354:1399	The method may uncover the roles of HS contributing to the diseases as well as provide a potential new set of biomarkers for disease diagnosis and prognosis.					
34355888	2	56	theme	inflammatory	266:277	arg1	response					279:286	inflammatory response	266:286	inflammatory response	266:286	HS plays a role in regulating cell differentiation/proliferation, embryonic development, blood coagulation, inflammatory response, and viral infection.					
34355888	7	57	dep	amount	914:919	arg1	the					910:912	the	910:912	the	910:912	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	7	58	theme	HS	849:850	arg1	standards					874:882	authentic 13C-labeled HS disaccharide internal standards	827:882	authentic 13C-labeled HS disaccharide internal standards	827:882	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34355888	3	59	theme	HS	324:325	arg1	HS					324:325	HS	324:325	HS	324:325	The amount of HS and its structural information are critically important for investigating the functions of HS in vivo.					
34355888	3	59	theme	HS	324:325	arg1	amount					314:319	The amount	310:319	The amount of HS and its structural information	310:356	The amount of HS and its structural information are critically important for investigating the functions of HS in vivo.					
34355888	3	59	theme	HS	324:325	arg1	information					346:356	its structural information	331:356	its structural information	331:356	The amount of HS and its structural information are critically important for investigating the functions of HS in vivo.					
34355888	3	59	theme	HS	324:325	arg1	important					373:381	important	373:381	important	373:381	The amount of HS and its structural information are critically important for investigating the functions of HS in vivo.					
34355888	2	60	theme	blood	247:251	arg1	coagulation					253:263	blood coagulation	247:263	blood coagulation	247:263	HS plays a role in regulating cell differentiation/proliferation, embryonic development, blood coagulation, inflammatory response, and viral infection.					
34355888	0	61	theme	Biological	63:72	arg1	Samples					74:80	Biological Samples	63:80	Biological Samples	63:80	Improving the Sensitivity for Quantifying Heparan Sulfate from Biological Samples.					
34355888	4	62	theme	HS	498:499	arg1	analysis					486:493	the analysis	482:493	the analysis of HS from biological samples	482:523	A sensitive and reliable quantitative technique for the analysis of HS from biological samples is under development.					
34355888	7	63	theme	histological	976:987	arg1	slide					989:993	a single histological slide	967:993	a single histological slide	967:993	The use of AMQC coupled with authentic 13C-labeled HS disaccharide internal standards empowered us to determine the amount and the disaccharide composition of the HS on a single histological slide.					
34085260	4	0	theme	HEK293S	866:872	arg1	line					885:888	the HEK293S GnTI- cell line	862:888	the HEK293S GnTI- cell line	862:888	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	4	0	theme	HEK293S	866:872	arg1	derivative					902:911	an HEK293S derivative	891:911	an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans	891:1015	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	1	1	theme	structure	150:158	arg1	studies					173:179	structure and function studies	150:179	structure and function studies where there is a need for milligram amounts of protein in pure form	150:247	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	1	2	theme	pure	239:242	arg1	form					244:247	pure form	239:247	pure form	239:247	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	3	3	theme	favorable	510:518	arg1	properties					520:529	several favorable properties	502:529	several favorable properties as a recombinant host including	502:561	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	4	theme	membrane	785:792	arg1	proteins					794:801	complex membrane proteins	777:801	complex membrane proteins such as GPCRs	777:815	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	4	theme	membrane	785:792	arg1	GPCRs					811:815	GPCRs	811:815	GPCRs	811:815	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	4	5	theme	HEK293S	894:900	arg1	line					885:888	the HEK293S GnTI- cell line	862:888	the HEK293S GnTI- cell line	862:888	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	4	5	theme	HEK293S	894:900	arg1	derivative					902:911	an HEK293S derivative	891:911	an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans	891:1015	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	1	6	theme	Large-scale	60:70	arg1	expression					84:93	Large-scale recombinant expression	60:93	Large-scale recombinant expression of G protein-coupled receptors (GPCRs)	60:132	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	3	7	theme	proteins	794:801	arg1	processing					763:772	processing	763:772	processing	763:772	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	7	theme	proteins	794:801	arg1	folding					751:757	correct folding	743:757	correct folding	743:757	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	8	theme	necessary	679:687	arg1	co-					689:691	the necessary co-	675:691	the necessary co-	675:691	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	1	9	theme	recombinant	72:82	arg1	expression					84:93	Large-scale recombinant expression	60:93	Large-scale recombinant expression of G protein-coupled receptors (GPCRs)	60:132	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	1	10	theme	function	164:171	arg1	studies					173:179	structure and function studies	150:179	structure and function studies where there is a need for milligram amounts of protein in pure form	150:247	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	4	11	theme	glycoproteins	955:967	arg1	production					941:950	the production	937:950	the production of glycoproteins modified homogeneously with truncated N-glycans	937:1015	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	3	12	theme	several	502:508	arg1	properties					520:529	several favorable properties	502:529	several favorable properties as a recombinant host including	502:561	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	2	13	theme	lines	353:357	arg1	construction					287:298	the construction	283:298	the construction of human embryonic kidney 293S (HEK293S) stable cell lines	283:357	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	3	14	theme	recombinant	536:546	arg1	host					548:551	a recombinant host	534:551	a recombinant host including	534:561	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	2	15	theme	cell	348:351	arg1	lines					353:357	human embryonic kidney 293S (HEK293S) stable cell lines	303:357	human embryonic kidney 293S (HEK293S) stable cell lines	303:357	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	2	16	theme	bovine	405:410	arg1	rhodopsin					412:420	bovine rhodopsin	405:420	bovine rhodopsin	405:420	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	3	17	theme	post-translational	697:714	arg1	modifications					716:728	post-translational modifications	697:728	post-translational modifications	697:728	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	2	18	theme	stable	341:346	arg1	lines					353:357	human embryonic kidney 293S (HEK293S) stable cell lines	303:357	human embryonic kidney 293S (HEK293S) stable cell lines	303:357	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	0	19	theme	Cell	28:31	arg1	Lines					33:37	Recombinant Cell Lines	16:37	Recombinant Cell Lines	16:37	Construction of Recombinant Cell Lines for GPCR Expression.					
34085260	1	20	theme	protein-coupled	100:114	arg1	GPCRs					127:131	GPCRs	127:131	GPCRs	127:131	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	1	20	theme	protein-coupled	100:114	arg1	receptors					116:124	G protein-coupled receptors	98:124	G protein-coupled receptors (GPCRs)	98:132	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	3	21	theme	HEK293S	427:433	arg1	line					440:443	The HEK293S cell line	423:443	The HEK293S cell line	423:443	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	21	theme	HEK293S	427:433	arg1	suitable					461:468	suitable	461:468	suitable	461:468	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	2	22	theme	gene	391:394	arg1	expression					373:382	inducible expression	363:382	inducible expression of the gene encoding bovine rhodopsin	363:420	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	4	23	theme	cell	880:883	arg1	line					885:888	the HEK293S GnTI- cell line	862:888	the HEK293S GnTI- cell line	862:888	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	4	23	theme	cell	880:883	arg1	derivative					902:911	an HEK293S derivative	891:911	an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans	891:1015	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	3	24	theme	handling	607:614	arg1	amounts					622:628	handling large amounts	607:628	handling large amounts of protein cargo	607:645	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	24	theme	handling	607:614	arg1	cargo					641:645	protein cargo	633:645	protein cargo	633:645	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	1	25	theme	receptors	116:124	arg1	expression					84:93	Large-scale recombinant expression	60:93	Large-scale recombinant expression of G protein-coupled receptors (GPCRs)	60:132	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	3	26	theme	cell	435:438	arg1	line					440:443	The HEK293S cell line	423:443	The HEK293S cell line	423:443	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	26	theme	cell	435:438	arg1	suitable					461:468	suitable	461:468	suitable	461:468	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	27	theme	large	616:620	arg1	amounts					622:628	handling large amounts	607:628	handling large amounts of protein cargo	607:645	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	27	theme	large	616:620	arg1	cargo					641:645	protein cargo	633:645	protein cargo	633:645	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	0	28	theme	Lines	33:37	arg1	Construction					0:11	Construction	0:11	Construction of Recombinant Cell Lines for GPCR Expression.	0:58	Construction of Recombinant Cell Lines for GPCR Expression.					
34085260	3	29	theme	correct	743:749	arg1	folding					751:757	correct folding	743:757	correct folding	743:757	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	2	30	theme	inducible	363:371	arg1	expression					373:382	inducible expression	363:382	inducible expression of the gene encoding bovine rhodopsin	363:420	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	4	31	theme	truncated	997:1005	arg1	N-glycans					1007:1015	truncated N-glycans	997:1015	truncated N-glycans	997:1015	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	4	32	used	used	928:931	arg2	line					885:888	the HEK293S GnTI- cell line	862:888	the HEK293S GnTI- cell line	862:888	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	4	32	used	used	928:931	arg2	derivative					902:911	an HEK293S derivative	891:911	an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans	891:1015	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	2	33	theme	kidney	319:324	arg1	lines					353:357	human embryonic kidney 293S (HEK293S) stable cell lines	303:357	human embryonic kidney 293S (HEK293S) stable cell lines	303:357	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	0	34	theme	GPCR	43:46	arg1	Expression					48:57	GPCR Expression	43:57	GPCR Expression	43:57	Construction of Recombinant Cell Lines for GPCR Expression.					
34085260	3	35	theme	protein	633:639	arg1	cargo					641:645	protein cargo	633:645	protein cargo	633:645	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	2	36	theme	embryonic	309:317	arg1	kidney					319:324	human embryonic kidney 293S	303:329	human embryonic kidney 293S (HEK293S) stable cell lines	303:357	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	2	36	theme	embryonic	309:317	arg1	HEK293S					332:338	HEK293S	332:338	HEK293S	332:338	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	3	37	theme	transfection	576:587	arg1	ease					568:571	its ease	564:571	its ease of transfection	564:587	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	37	theme	transfection	576:587	arg1	capacity					594:601	its capacity	590:601	its capacity for handling large amounts of protein cargo	590:645	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	37	theme	transfection	576:587	arg1	properties					520:529	several favorable properties	502:529	several favorable properties as a recombinant host including	502:561	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	37	theme	transfection	576:587	arg1	ability					656:662	its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs	652:815	its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs	652:815	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	4	38	theme	GnTI-	874:878	arg1	line					885:888	the HEK293S GnTI- cell line	862:888	the HEK293S GnTI- cell line	862:888	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	4	38	theme	GnTI-	874:878	arg1	derivative					902:911	an HEK293S derivative	891:911	an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans	891:1015	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	3	39	theme	cargo	641:645	arg1	amounts					622:628	handling large amounts	607:628	handling large amounts of protein cargo	607:645	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	39	theme	cargo	641:645	arg1	cargo					641:645	protein cargo	633:645	protein cargo	633:645	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	2	40	theme	human	303:307	arg1	kidney					319:324	human embryonic kidney 293S	303:329	human embryonic kidney 293S (HEK293S) stable cell lines	303:357	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	2	40	theme	human	303:307	arg1	HEK293S					332:338	HEK293S	332:338	HEK293S	332:338	Here we describe a procedure for the construction of human embryonic kidney 293S (HEK293S) stable cell lines for inducible expression of the gene encoding bovine rhodopsin.					
34085260	1	41	theme	milligram	207:215	arg1	protein					228:234	protein	228:234	protein	228:234	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	1	41	theme	milligram	207:215	arg1	amounts					217:223	milligram amounts	207:223	milligram amounts of protein in pure form	207:247	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	1	42	from	amounts	217:223	arg1	form					244:247	pure form	239:247	pure form	239:247	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	3	43	theme	complex	777:783	arg1	proteins					794:801	complex membrane proteins	777:801	complex membrane proteins such as GPCRs	777:815	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	3	43	theme	complex	777:783	arg1	GPCRs					811:815	GPCRs	811:815	GPCRs	811:815	The HEK293S cell line is particularly suitable for this application because of several favorable properties as a recombinant host including: its ease of transfection, its capacity for handling large amounts of protein cargo, and its ability to perform the necessary co- and post-translational modifications required for correct folding and processing of complex membrane proteins such as GPCRs.					
34085260	4	44	gly	glycoproteins	955:967	arg1	glycoproteins					955:967	glycoproteins	955:967	glycoproteins modified homogeneously with truncated N-glycans	955:1015	The procedures described here will focus on the HEK293S GnTI- cell line, an HEK293S derivative that is widely used for the production of glycoproteins modified homogeneously with truncated N-glycans.					
34085260	0	45	theme	Recombinant	16:26	arg1	Lines					33:37	Recombinant Cell Lines	16:37	Recombinant Cell Lines	16:37	Construction of Recombinant Cell Lines for GPCR Expression.					
34085260	1	46	theme	protein	228:234	arg1	protein					228:234	protein	228:234	protein	228:234	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34085260	1	46	theme	protein	228:234	arg1	amounts					217:223	milligram amounts	207:223	milligram amounts of protein in pure form	207:247	Large-scale recombinant expression of G protein-coupled receptors (GPCRs) is required for structure and function studies where there is a need for milligram amounts of protein in pure form.					
34606371	6	0	theme	EPS	969:971	arg1	samples					973:979	EPS samples	969:979	EPS samples	969:979	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	11	1	theme	ectopic	1697:1703	arg1	expression					1705:1714	the ectopic expression	1693:1714	the ectopic expression of plasmid-borne wgaE	1693:1736	Pyruvylation was restored through the ectopic expression of plasmid-borne wgaE.					
34606371	9	2	theme	Most	1466:1469	arg1	samples					1471:1477	Most samples	1466:1477	Most samples	1466:1477	Most samples were similar in composition to wild-type EPSII by CPMAS NMR analysis.					
34606371	10	3	theme	significant	1620:1630	arg1	reduction					1632:1640	a significant reduction	1618:1640	a significant reduction in pyruvylation	1618:1656	However, galactoglucan produced from a strain lacking wgaE exhibited a significant reduction in pyruvylation.					
34606371	13	4	theme	solid-state	1884:1894	arg1	detection					1900:1908	solid-state NMR detection	1884:1908	solid-state NMR detection	1884:1908	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	11	5	theme	wgaE	1733:1736	arg1	expression					1705:1714	the ectopic expression	1693:1714	the ectopic expression of plasmid-borne wgaE	1693:1736	Pyruvylation was restored through the ectopic expression of plasmid-borne wgaE.					
34606371	19	6	theme	wgaE	2658:2661	arg1	mutant					2668:2673	a wgaE gene mutant	2656:2673	a wgaE gene mutant	2656:2673	NMR analysis of EPS isolated from a wgaE gene mutant revealed a novel pyruvyltransferase that modifies galactoglucan.					
34606371	8	7	dep	pyruvyltransferase	1427:1444	arg1	SMb21322					1455:1462	SMb21322	1455:1462	SMb21322	1455:1462	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	8	7	dep	pyruvyltransferase	1427:1444	arg1	wgaE					1447:1450	wgaE	1447:1450	wgaE	1447:1450	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	13	8	theme	systematic	1837:1846	arg1	combination					1848:1858	the systematic combination	1833:1858	the systematic combination of genetic analyses and solid-state NMR detection	1833:1908	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	13	8	theme	systematic	1837:1846	arg1	means					1921:1925	a rapid means	1913:1925	a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides	1913:2004	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	7	9	theme	chemical	1137:1144	arg1	composition					1146:1156	chemical composition	1137:1156	chemical composition in complex samples	1137:1175	CPMAS NMR is uniquely suited to defining chemical composition in complex samples and enables the detection and quantification of distinct EPS functional groups.					
34606371	20	10	theme	Few	2740:2742	arg1	pyruvyltransferases					2748:2766	Few EPS pyruvyltransferases	2740:2766	Few EPS pyruvyltransferases	2740:2766	Few EPS pyruvyltransferases have been characterized.					
34606371	14	11	theme	IMPORTANCE	2007:2016	arg1	bacteria					2034:2041	IMPORTANCE Nitrogen-fixing bacteria	2007:2041	IMPORTANCE Nitrogen-fixing bacteria	2007:2041	IMPORTANCE Nitrogen-fixing bacteria are crucial for geochemical cycles and global nitrogen nutrition.					
34606371	6	12	theme	spinning	1033:1040	arg1	resonance					1079:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance	998:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR)	998:1093	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	6	12	theme	spinning	1033:1040	arg1	NMR					1090:1092	NMR	1090:1092	NMR	1090:1092	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	18	13	theme	intact	2564:2569	arg1	EPS					2571:2573	intact EPS	2564:2573	intact EPS from wild-type and mutant S. meliloti strains	2564:2619	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	8	14	theme	acyl/acetyltransferase	1337:1358	arg1	genes					1360:1364	five candidate acyl/acetyltransferase genes	1322:1364	five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016)	1322:1410	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	6	15	theme	cross-polarization	1002:1019	arg1	resonance					1079:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance	998:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR)	998:1093	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	6	15	theme	cross-polarization	1002:1019	arg1	NMR					1090:1092	NMR	1090:1092	NMR	1090:1092	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	16	16	theme	Sinorhizobium	2297:2309	arg1	meliloti					2311:2318	Sinorhizobium meliloti	2297:2318	Sinorhizobium meliloti (succinoglycan and galactoglucan)	2297:2352	Secreted exopolysaccharides (EPSs) produced by Sinorhizobium meliloti (succinoglycan and galactoglucan) play important roles in soil and plant environments.					
34606371	19	17	theme	gene	2663:2666	arg1	mutant					2668:2673	a wgaE gene mutant	2656:2673	a wgaE gene mutant	2656:2673	NMR analysis of EPS isolated from a wgaE gene mutant revealed a novel pyruvyltransferase that modifies galactoglucan.					
34606371	16	18	dep	meliloti	2311:2318	arg1	galactoglucan					2339:2351	galactoglucan	2339:2351	galactoglucan	2339:2351	Secreted exopolysaccharides (EPSs) produced by Sinorhizobium meliloti (succinoglycan and galactoglucan) play important roles in soil and plant environments.					
34606371	16	18	dep	meliloti	2311:2318	arg1	succinoglycan					2321:2333	succinoglycan	2321:2333	succinoglycan	2321:2333	Secreted exopolysaccharides (EPSs) produced by Sinorhizobium meliloti (succinoglycan and galactoglucan) play important roles in soil and plant environments.					
34606371	9	19	theme	CPMAS	1529:1533	arg1	analysis					1539:1546	CPMAS NMR analysis	1529:1546	CPMAS NMR analysis	1529:1546	Most samples were similar in composition to wild-type EPSII by CPMAS NMR analysis.					
34606371	3	20	theme	succinoglycan	604:616	arg1	absence					593:599	the absence	589:599	the absence of succinoglycan on some host plants	589:636	Galactoglucan is critical for population-based behaviors such as swarming and biofilm formation and can facilitate invasion in the absence of succinoglycan on some host plants.					
34606371	8	21	from	deletions	1309:1317	arg1	pyruvyltransferase					1427:1444	a putative pyruvyltransferase	1416:1444	a putative pyruvyltransferase (wgaE or SMb21322)	1416:1463	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	8	21	from	deletions	1309:1317	arg1	genes					1360:1364	five candidate acyl/acetyltransferase genes	1322:1364	five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016)	1322:1410	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	8	22	attach	isolated	1275:1282	arg1	strains					1296:1302	mutant strains	1289:1302	mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322)	1289:1463	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	8	22	attach	isolated	1275:1282	arg2	Galactoglucan					1257:1269	Galactoglucan	1257:1269	Galactoglucan	1257:1269	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	18	23	theme	nuclear	2520:2526	arg1	NMR					2548:2550	NMR	2548:2550	NMR	2548:2550	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	18	23	theme	nuclear	2520:2526	arg1	resonance					2537:2545	solid-state nuclear magnetic resonance	2508:2545	solid-state nuclear magnetic resonance (NMR)	2508:2551	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	3	24	from	absence	593:599	arg1	plants					631:636	some host plants	621:636	some host plants	621:636	Galactoglucan is critical for population-based behaviors such as swarming and biofilm formation and can facilitate invasion in the absence of succinoglycan on some host plants.					
34606371	0	25	theme	Nuclear	67:73	arg1	Resonance					84:92	13C Solid-State Nuclear Magnetic Resonance	51:92	13C Solid-State Nuclear Magnetic Resonance	51:92	Identification of a Novel Pyruvyltransferase Using 13C Solid-State Nuclear Magnetic Resonance To Analyze Rhizobial Exopolysaccharides.					
34606371	19	26	attach	isolated	2642:2649	arg1	mutant					2668:2673	a wgaE gene mutant	2656:2673	a wgaE gene mutant	2656:2673	NMR analysis of EPS isolated from a wgaE gene mutant revealed a novel pyruvyltransferase that modifies galactoglucan.					
34606371	19	26	attach	isolated	2642:2649	arg2	EPS					2638:2640	EPS	2638:2640	EPS isolated from a wgaE gene mutant	2638:2673	NMR analysis of EPS isolated from a wgaE gene mutant revealed a novel pyruvyltransferase that modifies galactoglucan.					
34606371	16	27	theme	important	2359:2367	arg1	roles					2369:2373	important roles	2359:2373	important roles	2359:2373	Secreted exopolysaccharides (EPSs) produced by Sinorhizobium meliloti (succinoglycan and galactoglucan) play important roles in soil and plant environments.					
34606371	13	28	theme	rhizobial	1977:1985	arg1	exopolysaccharides					1987:2004	rhizobial exopolysaccharides	1977:2004	rhizobial exopolysaccharides	1977:2004	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	5	29	theme	bacterial	950:958	arg1	strains					960:966	mutant bacterial strains	943:966	mutant bacterial strains	943:966	We devised a pipeline to identify putative pyruvyltransferase and acetyltransferase genes, construct genomic deletions in strains engineered to produce either succinoglycan or galactoglucan, and analyze EPS from mutant bacterial strains.					
34606371	6	30	theme	solid-state	1050:1060	arg1	resonance					1079:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance	998:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR)	998:1093	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	6	30	theme	solid-state	1050:1060	arg1	NMR					1090:1092	NMR	1090:1092	NMR	1090:1092	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	8	31	dep	genes	1360:1364	arg1	exoH					1373:1376	exoH	1373:1376	exoH	1373:1376	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	8	31	dep	genes	1360:1364	arg1	exoZ					1367:1370	exoZ	1367:1370	exoZ	1367:1370	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	6	32	theme	CPMAS	1043:1047	arg1	resonance					1079:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance	998:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR)	998:1093	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	6	32	theme	CPMAS	1043:1047	arg1	NMR					1090:1092	NMR	1090:1092	NMR	1090:1092	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	16	33	theme	Secreted	2250:2257	arg1	EPSs					2279:2282	EPSs	2279:2282	EPSs	2279:2282	Secreted exopolysaccharides (EPSs) produced by Sinorhizobium meliloti (succinoglycan and galactoglucan) play important roles in soil and plant environments.					
34606371	16	33	theme	Secreted	2250:2257	arg1	exopolysaccharides					2259:2276	Secreted exopolysaccharides	2250:2276	Secreted exopolysaccharides (EPSs) produced by Sinorhizobium meliloti (succinoglycan and galactoglucan)	2250:2352	Secreted exopolysaccharides (EPSs) produced by Sinorhizobium meliloti (succinoglycan and galactoglucan) play important roles in soil and plant environments.					
34606371	13	34	theme	responsible	1945:1955	arg1	genes					1939:1943	genes	1939:1943	genes responsible for modification of rhizobial exopolysaccharides	1939:2004	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	5	35	theme	putative	765:772	arg1	pyruvyltransferase					774:791	putative pyruvyltransferase and acetyltransferase genes	765:819	pyruvyltransferase	774:791	We devised a pipeline to identify putative pyruvyltransferase and acetyltransferase genes, construct genomic deletions in strains engineered to produce either succinoglycan or galactoglucan, and analyze EPS from mutant bacterial strains.					
34606371	2	36	theme	nodules	453:459	arg1	formation					419:427	the formation	415:427	the formation of nitrogen-fixing root nodules	415:459	Succinoglycan is essential for invasion of plant hosts and, thus, for the formation of nitrogen-fixing root nodules.					
34606371	17	37	theme	galactoglucan	2427:2439	arg1	biosynthesis					2411:2422	The biosynthesis	2407:2422	The biosynthesis of galactoglucan	2407:2439	The biosynthesis of galactoglucan is not as well characterized as that of succinoglycan.					
34606371	17	37	theme	galactoglucan	2427:2439	arg1	that					2473:2476	that	2473:2476	that	2473:2476	The biosynthesis of galactoglucan is not as well characterized as that of succinoglycan.					
34606371	21	38	theme	important	2847:2855	arg1	EPS					2869:2871	an important S. meliloti EPS	2844:2871	an important S. meliloti EPS	2844:2871	Our work provides insight into the biosynthesis of an important S. meliloti EPS and expands the knowledge of enzymes that modify polysaccharides.					
34606371	8	39	theme	putative	1418:1425	arg1	pyruvyltransferase					1427:1444	a putative pyruvyltransferase	1416:1444	a putative pyruvyltransferase (wgaE or SMb21322)	1416:1463	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	7	40	theme	functional	1238:1247	arg1	groups					1249:1254	distinct EPS functional groups	1225:1254	distinct EPS functional groups	1225:1254	CPMAS NMR is uniquely suited to defining chemical composition in complex samples and enables the detection and quantification of distinct EPS functional groups.					
34606371	5	41	from	strains	960:966	arg1	EPS					934:936	EPS	934:936	EPS from mutant bacterial strains	934:966	We devised a pipeline to identify putative pyruvyltransferase and acetyltransferase genes, construct genomic deletions in strains engineered to produce either succinoglycan or galactoglucan, and analyze EPS from mutant bacterial strains.					
34606371	2	42	theme	nitrogen-fixing	432:446	arg1	nodules					453:459	nitrogen-fixing root nodules	432:459	nitrogen-fixing root nodules	432:459	Succinoglycan is essential for invasion of plant hosts and, thus, for the formation of nitrogen-fixing root nodules.					
34606371	21	43	theme	meliloti	2860:2867	arg1	EPS					2869:2871	an important S. meliloti EPS	2844:2871	an important S. meliloti EPS	2844:2871	Our work provides insight into the biosynthesis of an important S. meliloti EPS and expands the knowledge of enzymes that modify polysaccharides.					
34606371	7	44	theme	distinct	1225:1232	arg1	groups					1249:1254	distinct EPS functional groups	1225:1254	distinct EPS functional groups	1225:1254	CPMAS NMR is uniquely suited to defining chemical composition in complex samples and enables the detection and quantification of distinct EPS functional groups.					
34606371	0	45	theme	Pyruvyltransferase	26:43	arg1	Identification					0:13	Identification	0:13	Identification of a Novel Pyruvyltransferase Using 13C Solid-State Nuclear Magnetic Resonance	0:92	Identification of a Novel Pyruvyltransferase Using 13C Solid-State Nuclear Magnetic Resonance To Analyze Rhizobial Exopolysaccharides.					
34606371	14	46	theme	nitrogen	2089:2096	arg1	nutrition					2098:2106	global nitrogen nutrition	2082:2106	global nitrogen nutrition	2082:2106	IMPORTANCE Nitrogen-fixing bacteria are crucial for geochemical cycles and global nitrogen nutrition.					
34606371	0	47	theme	13C	51:53	arg1	Resonance					84:92	13C Solid-State Nuclear Magnetic Resonance	51:92	13C Solid-State Nuclear Magnetic Resonance	51:92	Identification of a Novel Pyruvyltransferase Using 13C Solid-State Nuclear Magnetic Resonance To Analyze Rhizobial Exopolysaccharides.					
34606371	1	48	theme	changing	323:330	arg1	environment					332:342	a changing environment	321:342	a changing environment	321:342	The alphaproteobacterium Sinorhizobium meliloti secretes two acidic exopolysaccharides (EPSs), succinoglycan (EPSI) and galactoglucan (EPSII), which differentially enable it to adapt to a changing environment.					
34606371	18	49	theme	wild-type	2580:2588	arg1	strains					2613:2619	wild-type and mutant S. meliloti strains	2580:2619	wild-type and mutant S. meliloti strains	2580:2619	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	19	50	theme	EPS	2638:2640	arg1	analysis					2626:2633	NMR analysis	2622:2633	NMR analysis of EPS isolated from a wgaE gene mutant	2622:2673	NMR analysis of EPS isolated from a wgaE gene mutant revealed a novel pyruvyltransferase that modifies galactoglucan.					
34606371	10	51	from	reduction	1632:1640	arg1	pyruvylation					1645:1656	pyruvylation	1645:1656	pyruvylation	1645:1656	However, galactoglucan produced from a strain lacking wgaE exhibited a significant reduction in pyruvylation.					
34606371	14	52	theme	geochemical	2059:2069	arg1	cycles					2071:2076	geochemical cycles	2059:2076	geochemical cycles	2059:2076	IMPORTANCE Nitrogen-fixing bacteria are crucial for geochemical cycles and global nitrogen nutrition.					
34606371	13	53	theme	NMR	1896:1898	arg1	detection					1900:1908	solid-state NMR detection	1884:1908	solid-state NMR detection	1884:1908	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	2	54	theme	plant	388:392	arg1	hosts					394:398	plant hosts	388:398	plant hosts	388:398	Succinoglycan is essential for invasion of plant hosts and, thus, for the formation of nitrogen-fixing root nodules.					
34606371	0	55	theme	Solid-State	55:65	arg1	Resonance					84:92	13C Solid-State Nuclear Magnetic Resonance	51:92	13C Solid-State Nuclear Magnetic Resonance	51:92	Identification of a Novel Pyruvyltransferase Using 13C Solid-State Nuclear Magnetic Resonance To Analyze Rhizobial Exopolysaccharides.					
34606371	6	56	theme	magnetic	1070:1077	arg1	resonance					1079:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance	998:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR)	998:1093	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	6	56	theme	magnetic	1070:1077	arg1	NMR					1090:1092	NMR	1090:1092	NMR	1090:1092	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	11	57	theme	plasmid-borne	1719:1731	arg1	wgaE					1733:1736	plasmid-borne wgaE	1719:1736	plasmid-borne wgaE	1719:1736	Pyruvylation was restored through the ectopic expression of plasmid-borne wgaE.					
34606371	18	58	from	strains	2613:2619	arg1	EPS					2571:2573	intact EPS	2564:2573	intact EPS from wild-type and mutant S. meliloti strains	2564:2619	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	21	59	theme	enzymes	2902:2908	arg1	knowledge					2889:2897	the knowledge	2885:2897	the knowledge of enzymes that modify polysaccharides	2885:2936	Our work provides insight into the biosynthesis of an important S. meliloti EPS and expands the knowledge of enzymes that modify polysaccharides.					
34606371	13	60	theme	genetic	1863:1869	arg1	analyses					1871:1878	genetic analyses	1863:1878	genetic analyses	1863:1878	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	7	61	theme	complex	1161:1167	arg1	samples					1169:1175	complex samples	1161:1175	complex samples	1161:1175	CPMAS NMR is uniquely suited to defining chemical composition in complex samples and enables the detection and quantification of distinct EPS functional groups.					
34606371	5	62	theme	mutant	943:948	arg1	strains					960:966	mutant bacterial strains	943:966	mutant bacterial strains	943:966	We devised a pipeline to identify putative pyruvyltransferase and acetyltransferase genes, construct genomic deletions in strains engineered to produce either succinoglycan or galactoglucan, and analyze EPS from mutant bacterial strains.					
34606371	14	63	theme	Nitrogen-fixing	2018:2032	arg1	bacteria					2034:2041	IMPORTANCE Nitrogen-fixing bacteria	2007:2041	IMPORTANCE Nitrogen-fixing bacteria	2007:2041	IMPORTANCE Nitrogen-fixing bacteria are crucial for geochemical cycles and global nitrogen nutrition.					
34606371	9	64	theme	wild-type	1510:1518	arg1	EPSII					1520:1524	wild-type EPSII	1510:1524	wild-type EPSII	1510:1524	Most samples were similar in composition to wild-type EPSII by CPMAS NMR analysis.					
34606371	6	65	theme	magic-angle	1021:1031	arg1	resonance					1079:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance	998:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR)	998:1093	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	6	65	theme	magic-angle	1021:1031	arg1	NMR					1090:1092	NMR	1090:1092	NMR	1090:1092	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	8	66	theme	candidate	1327:1335	arg1	genes					1360:1364	five candidate acyl/acetyltransferase genes	1322:1364	five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016)	1322:1410	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	19	67	theme	novel	2686:2690	arg1	pyruvyltransferase					2692:2709	a novel pyruvyltransferase	2684:2709	a novel pyruvyltransferase that modifies galactoglucan	2684:2737	NMR analysis of EPS isolated from a wgaE gene mutant revealed a novel pyruvyltransferase that modifies galactoglucan.					
34606371	20	68	theme	EPS	2744:2746	arg1	pyruvyltransferases					2748:2766	Few EPS pyruvyltransferases	2740:2766	Few EPS pyruvyltransferases	2740:2766	Few EPS pyruvyltransferases have been characterized.					
34606371	6	69	theme	13C	998:1000	arg1	resonance					1079:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance	998:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR)	998:1093	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	6	69	theme	13C	998:1000	arg1	NMR					1090:1092	NMR	1090:1092	NMR	1090:1092	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	4	70	theme	galactoglucan	659:671	arg1	biosynthesis					643:654	The biosynthesis	639:654	The biosynthesis of galactoglucan	639:671	The biosynthesis of galactoglucan is not as completely understood as that of succinoglycan.					
34606371	4	70	theme	galactoglucan	659:671	arg1	that					708:711	that	708:711	that	708:711	The biosynthesis of galactoglucan is not as completely understood as that of succinoglycan.					
34606371	7	71	theme	CPMAS	1096:1100	arg1	NMR					1102:1104	CPMAS NMR	1096:1104	CPMAS NMR	1096:1104	CPMAS NMR is uniquely suited to defining chemical composition in complex samples and enables the detection and quantification of distinct EPS functional groups.					
34606371	9	72	theme	NMR	1535:1537	arg1	analysis					1539:1546	CPMAS NMR analysis	1529:1546	CPMAS NMR analysis	1529:1546	Most samples were similar in composition to wild-type EPSII by CPMAS NMR analysis.					
34606371	8	73	theme	mutant	1289:1294	arg1	strains					1296:1302	mutant strains	1289:1302	mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322)	1289:1463	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	18	74	theme	magnetic	2528:2535	arg1	NMR					2548:2550	NMR	2548:2550	NMR	2548:2550	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	18	74	theme	magnetic	2528:2535	arg1	resonance					2537:2545	solid-state nuclear magnetic resonance	2508:2545	solid-state nuclear magnetic resonance (NMR)	2508:2551	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	8	75	with	strains	1296:1302	arg1	deletions					1309:1317	deletions	1309:1317	deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322)	1309:1463	Galactoglucan was isolated from mutant strains with deletions in five candidate acyl/acetyltransferase genes (exoZ, exoH, SMb20810, SMb21188, and SMa1016) and a putative pyruvyltransferase (wgaE or SMb21322).					
34606371	0	76	theme	Magnetic	75:82	arg1	Resonance					84:92	13C Solid-State Nuclear Magnetic Resonance	51:92	13C Solid-State Nuclear Magnetic Resonance	51:92	Identification of a Novel Pyruvyltransferase Using 13C Solid-State Nuclear Magnetic Resonance To Analyze Rhizobial Exopolysaccharides.					
34606371	3	77	theme	host	626:629	arg1	plants					631:636	some host plants	621:636	some host plants	621:636	Galactoglucan is critical for population-based behaviors such as swarming and biofilm formation and can facilitate invasion in the absence of succinoglycan on some host plants.					
34606371	12	78	theme	galactoglucan	1778:1790	arg1	WgaE					1768:1771	WgaE	1768:1771	WgaE	1768:1771	Our work has thus identified WgaE as a galactoglucan pyruvyltransferase.					
34606371	12	78	theme	galactoglucan	1778:1790	arg1	pyruvyltransferase					1792:1809	a galactoglucan pyruvyltransferase	1776:1809	a galactoglucan pyruvyltransferase	1776:1809	Our work has thus identified WgaE as a galactoglucan pyruvyltransferase.					
34606371	18	79	theme	solid-state	2508:2518	arg1	NMR					2548:2550	NMR	2548:2550	NMR	2548:2550	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	18	79	theme	solid-state	2508:2518	arg1	resonance					2537:2545	solid-state nuclear magnetic resonance	2508:2545	solid-state nuclear magnetic resonance (NMR)	2508:2551	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	5	80	theme	acetyltransferase	797:813	arg1	genes					815:819	putative pyruvyltransferase and acetyltransferase genes	765:819	genes	815:819	We devised a pipeline to identify putative pyruvyltransferase and acetyltransferase genes, construct genomic deletions in strains engineered to produce either succinoglycan or galactoglucan, and analyze EPS from mutant bacterial strains.					
34606371	5	81	from	deletions	840:848	arg1	strains					853:859	strains	853:859	strains engineered to produce either succinoglycan or galactoglucan	853:919	We devised a pipeline to identify putative pyruvyltransferase and acetyltransferase genes, construct genomic deletions in strains engineered to produce either succinoglycan or galactoglucan, and analyze EPS from mutant bacterial strains.					
34606371	1	82	theme	acidic	196:201	arg1	EPSs					223:226	EPSs	223:226	EPSs	223:226	The alphaproteobacterium Sinorhizobium meliloti secretes two acidic exopolysaccharides (EPSs), succinoglycan (EPSI) and galactoglucan (EPSII), which differentially enable it to adapt to a changing environment.					
34606371	1	82	theme	acidic	196:201	arg1	galactoglucan					255:267	galactoglucan	255:267	galactoglucan (EPSII)	255:275	The alphaproteobacterium Sinorhizobium meliloti secretes two acidic exopolysaccharides (EPSs), succinoglycan (EPSI) and galactoglucan (EPSII), which differentially enable it to adapt to a changing environment.					
34606371	1	82	theme	acidic	196:201	arg1	succinoglycan					230:242	succinoglycan	230:242	succinoglycan (EPSI)	230:249	The alphaproteobacterium Sinorhizobium meliloti secretes two acidic exopolysaccharides (EPSs), succinoglycan (EPSI) and galactoglucan (EPSII), which differentially enable it to adapt to a changing environment.					
34606371	1	82	theme	acidic	196:201	arg1	exopolysaccharides					203:220	two acidic exopolysaccharides	192:220	two acidic exopolysaccharides (EPSs)	192:227	The alphaproteobacterium Sinorhizobium meliloti secretes two acidic exopolysaccharides (EPSs), succinoglycan (EPSI) and galactoglucan (EPSII), which differentially enable it to adapt to a changing environment.					
34606371	6	83	theme	nuclear	1062:1068	arg1	resonance					1079:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance	998:1087	13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR)	998:1093	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	6	83	theme	nuclear	1062:1068	arg1	NMR					1090:1092	NMR	1090:1092	NMR	1090:1092	EPS samples were examined by 13C cross-polarization magic-angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR).					
34606371	21	84	theme	EPS	2869:2871	arg1	biosynthesis					2828:2839	the biosynthesis	2824:2839	the biosynthesis of an important S. meliloti EPS	2824:2871	Our work provides insight into the biosynthesis of an important S. meliloti EPS and expands the knowledge of enzymes that modify polysaccharides.					
34606371	13	85	theme	exopolysaccharides	1987:2004	arg1	modification					1961:1972	modification	1961:1972	modification of rhizobial exopolysaccharides	1961:2004	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	0	86	theme	Rhizobial	105:113	arg1	Exopolysaccharides					115:132	Rhizobial Exopolysaccharides	105:132	Rhizobial Exopolysaccharides	105:132	Identification of a Novel Pyruvyltransferase Using 13C Solid-State Nuclear Magnetic Resonance To Analyze Rhizobial Exopolysaccharides.					
34606371	16	87	theme	plant	2387:2391	arg1	environments					2393:2404	plant environments	2387:2404	plant environments	2387:2404	Secreted exopolysaccharides (EPSs) produced by Sinorhizobium meliloti (succinoglycan and galactoglucan) play important roles in soil and plant environments.					
34606371	7	88	theme	groups	1249:1254	arg1	quantification					1207:1220	quantification	1207:1220	quantification	1207:1220	CPMAS NMR is uniquely suited to defining chemical composition in complex samples and enables the detection and quantification of distinct EPS functional groups.					
34606371	7	88	theme	groups	1249:1254	arg1	detection					1193:1201	detection	1193:1201	detection	1193:1201	CPMAS NMR is uniquely suited to defining chemical composition in complex samples and enables the detection and quantification of distinct EPS functional groups.					
34606371	7	89	dep	detection	1193:1201	arg1	the					1189:1191	the	1189:1191	the	1189:1191	CPMAS NMR is uniquely suited to defining chemical composition in complex samples and enables the detection and quantification of distinct EPS functional groups.					
34606371	7	90	theme	EPS	1234:1236	arg1	groups					1249:1254	distinct EPS functional groups	1225:1254	distinct EPS functional groups	1225:1254	CPMAS NMR is uniquely suited to defining chemical composition in complex samples and enables the detection and quantification of distinct EPS functional groups.					
34606371	2	91	theme	root	448:451	arg1	nodules					453:459	nitrogen-fixing root nodules	432:459	nitrogen-fixing root nodules	432:459	Succinoglycan is essential for invasion of plant hosts and, thus, for the formation of nitrogen-fixing root nodules.					
34606371	21	92	theme	S.	2857:2858	arg1	EPS					2869:2871	an important S. meliloti EPS	2844:2871	an important S. meliloti EPS	2844:2871	Our work provides insight into the biosynthesis of an important S. meliloti EPS and expands the knowledge of enzymes that modify polysaccharides.					
34606371	13	93	theme	rapid	1915:1919	arg1	combination					1848:1858	the systematic combination	1833:1858	the systematic combination of genetic analyses and solid-state NMR detection	1833:1908	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	13	93	theme	rapid	1915:1919	arg1	means					1921:1925	a rapid means	1913:1925	a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides	1913:2004	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	2	94	theme	hosts	394:398	arg1	invasion					376:383	invasion	376:383	invasion of plant hosts	376:398	Succinoglycan is essential for invasion of plant hosts and, thus, for the formation of nitrogen-fixing root nodules.					
34606371	0	95	theme	Novel	20:24	arg1	Pyruvyltransferase					26:43	a Novel Pyruvyltransferase	18:43	a Novel Pyruvyltransferase Using 13C Solid-State Nuclear Magnetic Resonance	18:92	Identification of a Novel Pyruvyltransferase Using 13C Solid-State Nuclear Magnetic Resonance To Analyze Rhizobial Exopolysaccharides.					
34606371	15	96	theme	root	2168:2171	arg1	nodules					2173:2179	root nodules	2168:2179	root nodules	2168:2179	Symbioses between legumes and rhizobial bacteria establish root nodules, where bacteria convert dinitrogen to ammonia for plant utilization.					
34606371	7	97	from	composition	1146:1156	arg1	samples					1169:1175	complex samples	1161:1175	complex samples	1161:1175	CPMAS NMR is uniquely suited to defining chemical composition in complex samples and enables the detection and quantification of distinct EPS functional groups.					
34606371	18	98	theme	mutant	2594:2599	arg1	strains					2613:2619	wild-type and mutant S. meliloti strains	2580:2619	wild-type and mutant S. meliloti strains	2580:2619	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	15	99	theme	rhizobial	2139:2147	arg1	bacteria					2149:2156	rhizobial bacteria	2139:2156	rhizobial bacteria	2139:2156	Symbioses between legumes and rhizobial bacteria establish root nodules, where bacteria convert dinitrogen to ammonia for plant utilization.					
34606371	5	100	theme	genomic	832:838	arg1	deletions					840:848	genomic deletions	832:848	genomic deletions in strains engineered to produce either succinoglycan or galactoglucan	832:919	We devised a pipeline to identify putative pyruvyltransferase and acetyltransferase genes, construct genomic deletions in strains engineered to produce either succinoglycan or galactoglucan, and analyze EPS from mutant bacterial strains.					
34606371	19	101	theme	NMR	2622:2624	arg1	analysis					2626:2633	NMR analysis	2622:2633	NMR analysis of EPS isolated from a wgaE gene mutant	2622:2673	NMR analysis of EPS isolated from a wgaE gene mutant revealed a novel pyruvyltransferase that modifies galactoglucan.					
34606371	14	102	theme	global	2082:2087	arg1	nutrition					2098:2106	global nitrogen nutrition	2082:2106	global nitrogen nutrition	2082:2106	IMPORTANCE Nitrogen-fixing bacteria are crucial for geochemical cycles and global nitrogen nutrition.					
34606371	15	103	theme	plant	2231:2235	arg1	utilization					2237:2247	plant utilization	2231:2247	plant utilization	2231:2247	Symbioses between legumes and rhizobial bacteria establish root nodules, where bacteria convert dinitrogen to ammonia for plant utilization.					
34606371	18	104	theme	meliloti	2604:2611	arg1	strains					2613:2619	wild-type and mutant S. meliloti strains	2580:2619	wild-type and mutant S. meliloti strains	2580:2619	We employed solid-state nuclear magnetic resonance (NMR) to examine intact EPS from wild-type and mutant S. meliloti strains.					
34606371	3	105	theme	population-based	492:507	arg1	behaviors					509:517	population-based behaviors	492:517	population-based behaviors such as swarming and biofilm formation	492:556	Galactoglucan is critical for population-based behaviors such as swarming and biofilm formation and can facilitate invasion in the absence of succinoglycan on some host plants.					
34606371	3	105	theme	population-based	492:507	arg1	swarming					527:534	swarming	527:534	swarming	527:534	Galactoglucan is critical for population-based behaviors such as swarming and biofilm formation and can facilitate invasion in the absence of succinoglycan on some host plants.					
34606371	3	105	theme	population-based	492:507	arg1	formation					548:556	biofilm formation	540:556	biofilm formation	540:556	Galactoglucan is critical for population-based behaviors such as swarming and biofilm formation and can facilitate invasion in the absence of succinoglycan on some host plants.					
34606371	13	106	theme	detection	1900:1908	arg1	combination					1848:1858	the systematic combination	1833:1858	the systematic combination of genetic analyses and solid-state NMR detection	1833:1908	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	13	106	theme	detection	1900:1908	arg1	means					1921:1925	a rapid means	1913:1925	a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides	1913:2004	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	9	107	dep	EPSII	1520:1524	arg1	composition					1495:1505	composition	1495:1505	composition	1495:1505	Most samples were similar in composition to wild-type EPSII by CPMAS NMR analysis.					
34606371	13	108	theme	analyses	1871:1878	arg1	combination					1848:1858	the systematic combination	1833:1858	the systematic combination of genetic analyses and solid-state NMR detection	1833:1908	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	13	108	theme	analyses	1871:1878	arg1	means					1921:1925	a rapid means	1913:1925	a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides	1913:2004	This exemplifies how the systematic combination of genetic analyses and solid-state NMR detection is a rapid means to identify genes responsible for modification of rhizobial exopolysaccharides.					
34606371	3	109	theme	biofilm	540:546	arg1	formation					548:556	biofilm formation	540:556	biofilm formation	540:556	Galactoglucan is critical for population-based behaviors such as swarming and biofilm formation and can facilitate invasion in the absence of succinoglycan on some host plants.					
34599988	8	0	theme	practical	1565:1573	arg1	strategy					1575:1582	a novel and practical strategy	1553:1582	a novel and practical strategy to valorize SLS for valuable polysaccharide	1553:1626	This study identified a novel and practical strategy to valorize SLS for valuable polysaccharide.					
34599988	3	1	theme	SLS	530:532	arg1	polysaccharide					534:547	SLS polysaccharide	530:547	SLS polysaccharide (SP)	530:552	In this work, SLS polysaccharide (SP) was ultrasonically extracted by optimizing the process conditions with response surface methodology.					
34599988	3	1	theme	SLS	530:532	arg1	SP					550:551	SP	550:551	SP	550:551	In this work, SLS polysaccharide (SP) was ultrasonically extracted by optimizing the process conditions with response surface methodology.					
34599988	1	2	theme	health-promoting	282:297	arg1	benefit					299:305	its nutritional and health-promoting benefit	262:305	its nutritional and health-promoting benefit	262:305	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	0	3	theme	Process	87:93	arg1	precipitation					109:121	precipitation	109:121	precipitation	109:121	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	0	3	theme	Process	87:93	arg1	characterization					135:150	structural characterization	124:150	structural characterization	124:150	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	0	3	theme	Process	87:93	arg1	optimization					95:106	substrate: Process optimization	76:106	substrate: Process optimization	76:106	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	0	3	theme	Process	87:93	arg1	activity					168:175	antioxidant activity	156:175	antioxidant activity	156:175	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	5	4	theme	D-glucuronic	1220:1231	arg1	acid					1233:1236	D-glucuronic acid	1220:1236	D-glucuronic acid	1220:1236	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	6	5	theme	surface	1371:1377	arg1	morphology					1379:1388	the surface morphology	1367:1388	the surface morphology	1367:1388	These fractions also varied in the surface morphology, where SP80 was looser and more porous than SP40 and SP60.					
34599988	5	6	theme	optimal	958:964	arg1	temperature					1001:1011	an extraction temperature	987:1011	an extraction temperature of 50 °C	987:1020	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	6	theme	optimal	958:964	arg1	ratio					1038:1042	a liquid-solid ratio	1023:1042	a liquid-solid ratio of 30 mL/g	1023:1053	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	6	theme	optimal	958:964	arg1	power					1073:1077	an ultrasonic power	1059:1077	an ultrasonic power of 120 W. SP	1059:1090	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	6	theme	optimal	958:964	arg1	conditions					966:975	the optimal conditions	954:975	the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP	954:1090	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	3	7	with	conditions	609:618	arg1	methodology					642:652	response surface methodology	625:652	response surface methodology	625:652	In this work, SLS polysaccharide (SP) was ultrasonically extracted by optimizing the process conditions with response surface methodology.					
34599988	5	8	theme	typical	1290:1296	arg1	spectrum					1307:1314	a typical infrared spectrum	1288:1314	a typical infrared spectrum for polysaccharide	1288:1333	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	9	theme	similar	1163:1169	arg1	composition					1186:1196	a similar monosaccharide composition	1161:1196	a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide	1161:1333	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	10	theme	liquid-solid	1025:1036	arg1	ratio					1038:1042	a liquid-solid ratio	1023:1042	a liquid-solid ratio of 30 mL/g	1023:1053	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	2	11	theme	mushroom	321:328	arg1	production					330:339	the mushroom production	317:339	the mushroom production	317:339	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	5	12	theme	extraction	990:999	arg1	temperature					1001:1011	an extraction temperature	987:1011	an extraction temperature of 50 °C	987:1020	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	0	13	theme	structural	124:133	arg1	characterization					135:150	structural characterization	124:150	structural characterization	124:150	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	0	13	theme	structural	124:133	arg1	optimization					95:106	substrate: Process optimization	76:106	substrate: Process optimization	76:106	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	0	14	from	Lentinus	60:67	arg1	extraction					20:29	Ultrasound-assisted extraction	0:29	Ultrasound-assisted extraction of polysaccharide from spent Lentinus	0:67	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	4	15	theme	ethanol	670:676	arg1	precipitation					678:690	gradient ethanol precipitation	661:690	gradient ethanol precipitation	661:690	Using gradient ethanol precipitation, SP was separated into SP40, SP60 and SP80, and their monosaccharide composition, structural properties, and antioxidant potential were further characterized and compared.					
34599988	7	16	theme	strongest	1488:1496	arg1	activities					1510:1519	the strongest antioxidant activities	1484:1519	the strongest antioxidant activities	1484:1519	Furthermore, SP and SP80 displayed the strongest antioxidant activities in vitro.					
34599988	5	17	theme	infrared	1298:1305	arg1	spectrum					1307:1314	a typical infrared spectrum	1288:1314	a typical infrared spectrum for polysaccharide	1288:1333	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	4	18	theme	antioxidant	801:811	arg1	potential					813:821	antioxidant potential	801:821	antioxidant potential	801:821	Using gradient ethanol precipitation, SP was separated into SP40, SP60 and SP80, and their monosaccharide composition, structural properties, and antioxidant potential were further characterized and compared.					
34599988	1	19	theme	second-most	201:211	arg1	edodes					187:192	Lentinus edodes	178:192	Lentinus edodes	178:192	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	1	19	theme	second-most	201:211	arg1	worldwide					245:253	the second-most popular and cultivated mushroom worldwide	197:253	the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit	197:305	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	8	20	theme	valuable	1604:1611	arg1	polysaccharide					1613:1626	valuable polysaccharide	1604:1626	valuable polysaccharide	1604:1626	This study identified a novel and practical strategy to valorize SLS for valuable polysaccharide.					
34599988	2	21	dep	L.	373:374	arg1	edodes					376:381	edodes	376:381	edodes	376:381	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	5	22	theme	monosaccharide	1171:1184	arg1	composition					1186:1196	a similar monosaccharide composition	1161:1196	a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide	1161:1333	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	1	23	theme	popular	213:219	arg1	edodes					187:192	Lentinus edodes	178:192	Lentinus edodes	178:192	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	1	23	theme	popular	213:219	arg1	worldwide					245:253	the second-most popular and cultivated mushroom worldwide	197:253	the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit	197:305	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	0	24	theme	Ultrasound-assisted	0:18	arg1	extraction					20:29	Ultrasound-assisted extraction	0:29	Ultrasound-assisted extraction of polysaccharide from spent Lentinus	0:67	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	8	25	theme	novel	1555:1559	arg1	strategy					1575:1582	a novel and practical strategy	1553:1582	a novel and practical strategy to valorize SLS for valuable polysaccharide	1553:1626	This study identified a novel and practical strategy to valorize SLS for valuable polysaccharide.					
34599988	3	26	theme	process	601:607	arg1	conditions					609:618	the process conditions	597:618	the process conditions with response surface methodology	597:652	In this work, SLS polysaccharide (SP) was ultrasonically extracted by optimizing the process conditions with response surface methodology.					
34599988	2	27	theme	great	459:463	arg1	challenge					465:473	a great challenge	457:473	a great challenge	457:473	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	2	28	theme	vast	351:354	arg1	SLS					394:396	SLS	394:396	SLS	394:396	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	2	28	theme	vast	351:354	arg1	substrate					383:391	spent L. edodes substrate	367:391	spent L. edodes substrate (SLS)	367:397	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	2	28	theme	vast	351:354	arg1	amounts					356:362	vast amounts	351:362	vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization	351:513	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	1	29	theme	cultivated	225:234	arg1	edodes					187:192	Lentinus edodes	178:192	Lentinus edodes	178:192	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	1	29	theme	cultivated	225:234	arg1	worldwide					245:253	the second-most popular and cultivated mushroom worldwide	197:253	the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit	197:305	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	4	30	theme	structural	774:783	arg1	properties					785:794	structural properties	774:794	structural properties	774:794	Using gradient ethanol precipitation, SP was separated into SP40, SP60 and SP80, and their monosaccharide composition, structural properties, and antioxidant potential were further characterized and compared.					
34599988	1	31	theme	mushroom	236:243	arg1	edodes					187:192	Lentinus edodes	178:192	Lentinus edodes	178:192	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	1	31	theme	mushroom	236:243	arg1	worldwide					245:253	the second-most popular and cultivated mushroom worldwide	197:253	the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit	197:305	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	0	32	theme	spent	54:58	arg1	Lentinus					60:67	spent Lentinus	54:67	spent Lentinus	54:67	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	3	33	theme	response	625:632	arg1	methodology					642:652	response surface methodology	625:652	response surface methodology	625:652	In this work, SLS polysaccharide (SP) was ultrasonically extracted by optimizing the process conditions with response surface methodology.					
34599988	5	34	theme	total	887:891	arg1	content					908:914	the total polysaccharide content	883:914	the total polysaccharide content	883:914	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	0	35	theme	antioxidant	156:166	arg1	activity					168:175	antioxidant activity	156:175	antioxidant activity	156:175	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	0	35	theme	antioxidant	156:166	arg1	optimization					95:106	substrate: Process optimization	76:106	substrate: Process optimization	76:106	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	0	36	theme	polysaccharide	34:47	arg1	extraction					20:29	Ultrasound-assisted extraction	0:29	Ultrasound-assisted extraction of polysaccharide from spent Lentinus	0:67	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	1	37	theme	due	255:257	arg1	edodes					187:192	Lentinus edodes	178:192	Lentinus edodes	178:192	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	1	37	theme	due	255:257	arg1	worldwide					245:253	the second-most popular and cultivated mushroom worldwide	197:253	the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit	197:305	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	2	38	theme	by-product	491:500	arg1	valorization					502:513	mushroom by-product valorization	482:513	mushroom by-product valorization	482:513	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	5	39	theme	D-galacturonic	1239:1252	arg1	acid					1254:1257	D-galacturonic acid	1239:1257	D-galacturonic acid	1239:1257	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	40	theme	120 W.	1082:1087	arg1	SP					1089:1090	120 W. SP	1082:1090	120 W. SP	1082:1090	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	2	41	theme	substrate	383:391	arg1	SLS					394:396	SLS	394:396	SLS	394:396	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	2	41	theme	substrate	383:391	arg1	substrate					383:391	spent L. edodes substrate	367:391	spent L. edodes substrate (SLS)	367:397	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	2	41	theme	substrate	383:391	arg1	amounts					356:362	vast amounts	351:362	vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization	351:513	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	2	42	theme	mushroom	482:489	arg1	valorization					502:513	mushroom by-product valorization	482:513	mushroom by-product valorization	482:513	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	5	43	theme	SP	1089:1090	arg1	temperature					1001:1011	an extraction temperature	987:1011	an extraction temperature of 50 °C	987:1020	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	43	theme	SP	1089:1090	arg1	ratio					1038:1042	a liquid-solid ratio	1023:1042	a liquid-solid ratio of 30 mL/g	1023:1053	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	43	theme	SP	1089:1090	arg1	power					1073:1077	an ultrasonic power	1059:1077	an ultrasonic power of 120 W. SP	1059:1090	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	44	theme	30 mL/g	1047:1053	arg1	temperature					1001:1011	an extraction temperature	987:1011	an extraction temperature of 50 °C	987:1020	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	44	theme	30 mL/g	1047:1053	arg1	ratio					1038:1042	a liquid-solid ratio	1023:1042	a liquid-solid ratio of 30 mL/g	1023:1053	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	44	theme	30 mL/g	1047:1053	arg1	power					1073:1077	an ultrasonic power	1059:1077	an ultrasonic power of 120 W. SP	1059:1090	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	3	45	theme	surface	634:640	arg1	methodology					642:652	response surface methodology	625:652	response surface methodology	625:652	In this work, SLS polysaccharide (SP) was ultrasonically extracted by optimizing the process conditions with response surface methodology.					
34599988	2	46	theme	L.	373:374	arg1	SLS					394:396	SLS	394:396	SLS	394:396	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	2	46	theme	L.	373:374	arg1	substrate					383:391	spent L. edodes substrate	367:391	spent L. edodes substrate (SLS)	367:397	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	5	47	theme	50 °C	1016:1020	arg1	temperature					1001:1011	an extraction temperature	987:1011	an extraction temperature of 50 °C	987:1020	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	47	theme	50 °C	1016:1020	arg1	ratio					1038:1042	a liquid-solid ratio	1023:1042	a liquid-solid ratio of 30 mL/g	1023:1053	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	47	theme	50 °C	1016:1020	arg1	power					1073:1077	an ultrasonic power	1059:1077	an ultrasonic power of 120 W. SP	1059:1090	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	7	48	theme	antioxidant	1498:1508	arg1	activities					1510:1519	the strongest antioxidant activities	1484:1519	the strongest antioxidant activities	1484:1519	Furthermore, SP and SP80 displayed the strongest antioxidant activities in vitro.					
34599988	5	49	theme	fractional	1100:1109	arg1	precipitations					1111:1124	its fractional precipitations	1096:1124	its fractional precipitations	1096:1124	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	2	50	theme	spent	367:371	arg1	SLS					394:396	SLS	394:396	SLS	394:396	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	2	50	theme	spent	367:371	arg1	substrate					383:391	spent L. edodes substrate	367:391	spent L. edodes substrate (SLS)	367:397	However, the mushroom production generates vast amounts of spent L. edodes substrate (SLS) that is generally discharged into the environment, posing a great challenge within mushroom by-product valorization.					
34599988	4	51	theme	monosaccharide	746:759	arg1	composition					761:771	their monosaccharide composition	740:771	their monosaccharide composition	740:771	Using gradient ethanol precipitation, SP was separated into SP40, SP60 and SP80, and their monosaccharide composition, structural properties, and antioxidant potential were further characterized and compared.					
34599988	5	52	theme	polysaccharide	893:906	arg1	content					908:914	the total polysaccharide content	883:914	the total polysaccharide content	883:914	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	5	53	dep	showed	876:881	arg1	reached					916:922	reached	916:922	showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP	876:1090	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	4	54	theme	gradient	661:668	arg1	precipitation					678:690	gradient ethanol precipitation	661:690	gradient ethanol precipitation	661:690	Using gradient ethanol precipitation, SP was separated into SP40, SP60 and SP80, and their monosaccharide composition, structural properties, and antioxidant potential were further characterized and compared.					
34599988	1	55	theme	nutritional	266:276	arg1	benefit					299:305	its nutritional and health-promoting benefit	262:305	its nutritional and health-promoting benefit	262:305	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	0	56	theme	substrate	76:84	arg1	precipitation					109:121	precipitation	109:121	precipitation	109:121	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	0	56	theme	substrate	76:84	arg1	characterization					135:150	structural characterization	124:150	structural characterization	124:150	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	0	56	theme	substrate	76:84	arg1	optimization					95:106	substrate: Process optimization	76:106	substrate: Process optimization	76:106	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	0	56	theme	substrate	76:84	arg1	activity					168:175	antioxidant activity	156:175	antioxidant activity	156:175	Ultrasound-assisted extraction of polysaccharide from spent Lentinus edodes substrate: Process optimization, precipitation, structural characterization and antioxidant activity.					
34599988	5	57	theme	ultrasonic	1062:1071	arg1	power					1073:1077	an ultrasonic power	1059:1077	an ultrasonic power of 120 W. SP	1059:1090	The results showed the total polysaccharide content reached up to 37.05 ± 0.31 mg/g under the optimal conditions including an extraction temperature of 50 °C, a liquid-solid ratio of 30 mL/g and an ultrasonic power of 120 W. SP and its fractional precipitations were heteropolysaccharides sharing a similar monosaccharide composition including L-rhamnose, D-glucuronic acid, D-galacturonic acid, d-glucose and D-xylose, and a typical infrared spectrum for polysaccharide.					
34599988	1	58	theme	Lentinus	178:185	arg1	worldwide					245:253	the second-most popular and cultivated mushroom worldwide	197:253	the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit	197:305	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
34599988	1	58	theme	Lentinus	178:185	arg1	edodes					187:192	Lentinus edodes	178:192	Lentinus edodes	178:192	Lentinus edodes is the second-most popular and cultivated mushroom worldwide due to its nutritional and health-promoting benefit.					
33898974	4	0	from	membrane	677:684	arg1	cells					697:701	fluidic cells	689:701	fluidic cells	689:701	We designed a solid-state nanopore sensor supported by a free-standing SiN membrane in fluidic cells.					
33898974	5	1	theme	hyperacetylated	832:846	arg1	modifications					865:877	hyperacetylated and unacetylated modifications	832:877	modifications	865:877	This device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/μL and discriminate xylans with hyperacetylated and unacetylated modifications.					
33898974	7	2	theme	new	1162:1164	arg1	avenue					1166:1171	a new avenue	1160:1171	a new avenue for understanding cell wall structures, and expanding polysaccharide applications	1160:1253	Combining the data for categorizing polysaccharide mixtures, our study establishes a single-molecule platform for polysaccharide analysis, opening a new avenue for understanding cell wall structures, and expanding polysaccharide applications.					
33898974	0	3	theme	polysaccharides	95:109	arg1	characterization					69:84	label-free characterization	58:84	label-free characterization of plant polysaccharides	58:109	A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides.					
33898974	7	4	theme	polysaccharide	1227:1240	arg1	applications					1242:1253	polysaccharide applications	1227:1253	polysaccharide applications	1227:1253	Combining the data for categorizing polysaccharide mixtures, our study establishes a single-molecule platform for polysaccharide analysis, opening a new avenue for understanding cell wall structures, and expanding polysaccharide applications.					
33898974	2	5	theme	polysaccharide	309:322	arg1	analysis					324:331	polysaccharide analysis	309:331	polysaccharide analysis	309:331	In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility, our knowledge of cell wall architecture and function is greatly constrained.					
33898974	4	6	theme	free-standing	659:671	arg1	membrane					677:684	a free-standing SiN membrane	657:684	a free-standing SiN membrane in fluidic cells	657:701	We designed a solid-state nanopore sensor supported by a free-standing SiN membrane in fluidic cells.					
33898974	6	7	theme	dicot	999:1003	arg1	plants					1005:1010	monocot and dicot plants	987:1010	monocot and dicot plants	987:1010	We further demonstrated the capability of this method in distinguishing arabinoxylan and glucuronoxylan in monocot and dicot plants.					
33898974	5	8	from	concentrations	770:783	arg1	xylans					760:765	cell wall polysaccharide xylans	735:765	cell wall polysaccharide xylans at concentrations as low as 5 ng/μL	735:801	This device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/μL and discriminate xylans with hyperacetylated and unacetylated modifications.					
33898974	2	9	theme	methodology	293:303	arg1	absence					269:275	the absence	265:275	the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility	265:403	In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility, our knowledge of cell wall architecture and function is greatly constrained.					
33898974	6	10	theme	monocot	987:993	arg1	plants					1005:1010	monocot and dicot plants	987:1010	monocot and dicot plants	987:1010	We further demonstrated the capability of this method in distinguishing arabinoxylan and glucuronoxylan in monocot and dicot plants.					
33898974	2	11	theme	effective	283:291	arg1	methodology					293:303	an effective methodology	280:303	an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility	280:403	In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility, our knowledge of cell wall architecture and function is greatly constrained.					
33898974	2	12	theme	wall	428:431	arg1	knowledge					410:418	our knowledge	406:418	our knowledge of cell wall architecture and function	406:457	In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility, our knowledge of cell wall architecture and function is greatly constrained.					
33898974	0	13	theme	solid-state	2:12	arg1	approach					45:52	A solid-state nanopore-based single-molecule approach	0:52	A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides	0:109	A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides.					
33898974	7	14	theme	polysaccharide	1049:1062	arg1	mixtures					1064:1071	polysaccharide mixtures	1049:1071	polysaccharide mixtures	1049:1071	Combining the data for categorizing polysaccharide mixtures, our study establishes a single-molecule platform for polysaccharide analysis, opening a new avenue for understanding cell wall structures, and expanding polysaccharide applications.					
33898974	2	15	theme	cell	423:426	arg1	wall					428:431	cell wall architecture and function	423:457	wall	428:431	In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility, our knowledge of cell wall architecture and function is greatly constrained.					
33898974	4	16	theme	solid-state	616:626	arg1	sensor					637:642	a solid-state nanopore sensor	614:642	a solid-state nanopore sensor supported by a free-standing SiN membrane in fluidic cells	614:701	We designed a solid-state nanopore sensor supported by a free-standing SiN membrane in fluidic cells.					
33898974	7	17	dep	establishes	1084:1094	arg1	opening					1152:1158	opening	1152:1158	opening	1152:1158	Combining the data for categorizing polysaccharide mixtures, our study establishes a single-molecule platform for polysaccharide analysis, opening a new avenue for understanding cell wall structures, and expanding polysaccharide applications.					
33898974	0	18	theme	single-molecule	29:43	arg1	approach					45:52	A solid-state nanopore-based single-molecule approach	0:52	A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides	0:109	A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides.					
33898974	7	19	theme	cell	1191:1194	arg1	structures					1201:1210	cell wall structures	1191:1210	cell wall structures	1191:1210	Combining the data for categorizing polysaccharide mixtures, our study establishes a single-molecule platform for polysaccharide analysis, opening a new avenue for understanding cell wall structures, and expanding polysaccharide applications.					
33898974	1	20	theme	important	132:140	arg1	Polysaccharides					112:126	Polysaccharides	112:126	Polysaccharides	112:126	Polysaccharides are important biomacromolecules existing in all plants, most of which are integrated into a fibrillar structure called the cell wall.					
33898974	1	20	theme	important	132:140	arg1	biomacromolecules					142:158	important biomacromolecules	132:158	important biomacromolecules	132:158	Polysaccharides are important biomacromolecules existing in all plants, most of which are integrated into a fibrillar structure called the cell wall.					
33898974	1	21	theme	fibrillar	220:228	arg1	structure					230:238	a fibrillar structure	218:238	a fibrillar structure called the cell wall	218:259	Polysaccharides are important biomacromolecules existing in all plants, most of which are integrated into a fibrillar structure called the cell wall.					
33898974	0	22	theme	nanopore-based	14:27	arg1	approach					45:52	A solid-state nanopore-based single-molecule approach	0:52	A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides	0:109	A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides.					
33898974	7	23	theme	wall	1196:1199	arg1	structures					1201:1210	cell wall structures	1191:1210	cell wall structures	1191:1210	Combining the data for categorizing polysaccharide mixtures, our study establishes a single-molecule platform for polysaccharide analysis, opening a new avenue for understanding cell wall structures, and expanding polysaccharide applications.					
33898974	3	24	theme	single-molecule	502:516	arg1	approach					518:525	a single-molecule approach	500:525	a single-molecule approach for identifying plant polysaccharides with acetylated modification levels	500:599	Here, we develop a single-molecule approach for identifying plant polysaccharides with acetylated modification levels.					
33898974	5	25	theme	unacetylated	852:863	arg1	modifications					865:877	hyperacetylated and unacetylated modifications	832:877	modifications	865:877	This device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/μL and discriminate xylans with hyperacetylated and unacetylated modifications.					
33898974	4	26	theme	nanopore	628:635	arg1	sensor					637:642	a solid-state nanopore sensor	614:642	a solid-state nanopore sensor supported by a free-standing SiN membrane in fluidic cells	614:701	We designed a solid-state nanopore sensor supported by a free-standing SiN membrane in fluidic cells.					
33898974	2	27	theme	function	450:457	arg1	knowledge					410:418	our knowledge	406:418	our knowledge of cell wall architecture and function	406:457	In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility, our knowledge of cell wall architecture and function is greatly constrained.					
33898974	5	28	theme	low	788:790	arg1	concentrations					770:783	concentrations	770:783	concentrations as low as 5 ng/μL	770:801	This device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/μL and discriminate xylans with hyperacetylated and unacetylated modifications.					
33898974	5	29	theme	cell	735:738	arg1	xylans					760:765	cell wall polysaccharide xylans	735:765	cell wall polysaccharide xylans at concentrations as low as 5 ng/μL	735:801	This device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/μL and discriminate xylans with hyperacetylated and unacetylated modifications.					
33898974	2	30	theme	structural	382:391	arg1	flexibility					393:403	structural flexibility	382:403	structural flexibility	382:403	In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility, our knowledge of cell wall architecture and function is greatly constrained.					
33898974	0	31	theme	label-free	58:67	arg1	characterization					69:84	label-free characterization	58:84	label-free characterization of plant polysaccharides	58:109	A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides.					
33898974	5	32	theme	wall	740:743	arg1	xylans					760:765	cell wall polysaccharide xylans	735:765	cell wall polysaccharide xylans at concentrations as low as 5 ng/μL	735:801	This device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/μL and discriminate xylans with hyperacetylated and unacetylated modifications.					
33898974	3	33	theme	plant	543:547	arg1	polysaccharides					549:563	plant polysaccharides	543:563	plant polysaccharides	543:563	Here, we develop a single-molecule approach for identifying plant polysaccharides with acetylated modification levels.					
33898974	5	34	theme	polysaccharide	745:758	arg1	xylans					760:765	cell wall polysaccharide xylans	735:765	cell wall polysaccharide xylans at concentrations as low as 5 ng/μL	735:801	This device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/μL and discriminate xylans with hyperacetylated and unacetylated modifications.					
33898974	6	35	theme	method	927:932	arg1	capability					908:917	the capability	904:917	the capability of this method	904:932	We further demonstrated the capability of this method in distinguishing arabinoxylan and glucuronoxylan in monocot and dicot plants.					
33898974	7	36	theme	polysaccharide	1127:1140	arg1	analysis					1142:1149	polysaccharide analysis	1127:1149	polysaccharide analysis	1127:1149	Combining the data for categorizing polysaccharide mixtures, our study establishes a single-molecule platform for polysaccharide analysis, opening a new avenue for understanding cell wall structures, and expanding polysaccharide applications.					
33898974	1	37	theme	cell	251:254	arg1	wall					256:259	the cell wall	247:259	the cell wall	247:259	Polysaccharides are important biomacromolecules existing in all plants, most of which are integrated into a fibrillar structure called the cell wall.					
33898974	7	38	theme	single-molecule	1098:1112	arg1	platform					1114:1121	a single-molecule platform	1096:1121	a single-molecule platform for polysaccharide analysis	1096:1149	Combining the data for categorizing polysaccharide mixtures, our study establishes a single-molecule platform for polysaccharide analysis, opening a new avenue for understanding cell wall structures, and expanding polysaccharide applications.					
33898974	2	39	theme	compositional	350:362	arg1	heterogeneity					364:376	compositional heterogeneity	350:376	compositional heterogeneity	350:376	In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility, our knowledge of cell wall architecture and function is greatly constrained.					
33898974	4	40	theme	SiN	673:675	arg1	membrane					677:684	a free-standing SiN membrane	657:684	a free-standing SiN membrane in fluidic cells	657:701	We designed a solid-state nanopore sensor supported by a free-standing SiN membrane in fluidic cells.					
33898974	0	41	theme	plant	89:93	arg1	polysaccharides					95:109	plant polysaccharides	89:109	plant polysaccharides	89:109	A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides.					
33898974	5	42	with	xylans	820:825	arg1	modifications					865:877	hyperacetylated and unacetylated modifications	832:877	modifications	865:877	This device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/μL and discriminate xylans with hyperacetylated and unacetylated modifications.					
33898974	3	43	theme	acetylated	570:579	arg1	levels					594:599	acetylated modification levels	570:599	acetylated modification levels	570:599	Here, we develop a single-molecule approach for identifying plant polysaccharides with acetylated modification levels.					
33898974	4	44	theme	fluidic	689:695	arg1	cells					697:701	fluidic cells	689:701	fluidic cells	689:701	We designed a solid-state nanopore sensor supported by a free-standing SiN membrane in fluidic cells.					
33898974	2	45	dep	wall	428:431	arg1	architecture					433:444	architecture	433:444	architecture	433:444	In the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility, our knowledge of cell wall architecture and function is greatly constrained.					
33898974	3	46	theme	modification	581:592	arg1	levels					594:599	acetylated modification levels	570:599	acetylated modification levels	570:599	Here, we develop a single-molecule approach for identifying plant polysaccharides with acetylated modification levels.					
34291950	0	0	theme	O-Polysaccharide	87:102	arg1	Unit					75:78	the All-Rare Sugar-Containing Pentasaccharide Repeating Unit	19:78	the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1)	19:158	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	1	1	theme	First	161:165	arg1	synthesis					173:181	First total synthesis	161:181	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1)	161:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	1	2	theme	total	167:171	arg1	synthesis					173:181	First total synthesis	161:181	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1)	161:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	1	3	theme	serotype	282:289	arg1	302-73					274:279	strain 302-73	267:279	Plesiomonas shigelloides strain 302-73 (serotype O1)	242:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	1	3	theme	serotype	282:289	arg1	O1					291:292	serotype O1	282:292	serotype O1	282:292	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	0	4	theme	Plesiomonas	107:117	arg1	shigelloides					119:130	Plesiomonas shigelloides Strain 302-73 (Serotype O1)	107:158	Plesiomonas shigelloides Strain 302-73 (Serotype O1)	107:158	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	3	5	theme	key	608:610	arg1	challenges					612:621	the key challenges	604:621	the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield	604:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	3	5	theme	key	608:610	arg1	nucleophilicity					519:533	The poor nucleophilicity	510:533	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations	510:598	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	3	6	from	challenges	612:621	arg1	synthesis					636:644	the total synthesis	626:644	the total synthesis	626:644	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	0	7	dep	shigelloides	119:130	arg1	302-73					139:144	Strain 302-73	132:144	Plesiomonas shigelloides Strain 302-73 (Serotype O1)	107:158	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	0	7	dep	shigelloides	119:130	arg1	O1					156:157	Serotype O1	147:157	Serotype O1	147:157	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	0	8	theme	Strain	132:137	arg1	302-73					139:144	Strain 302-73	132:144	Plesiomonas shigelloides Strain 302-73 (Serotype O1)	107:158	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	0	8	theme	Strain	132:137	arg1	O1					156:157	Serotype O1	147:157	Serotype O1	147:157	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	3	9	theme	4-OH	544:547	arg1	challenges					612:621	the key challenges	604:621	the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield	604:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	3	9	theme	4-OH	544:547	arg1	nucleophilicity					519:533	The poor nucleophilicity	510:533	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations	510:598	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	0	10	theme	shigelloides	119:130	arg1	O-Polysaccharide					87:102	the O-Polysaccharide	83:102	the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1)	83:158	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	3	11	from	yield	723:727	arg1	sequence					688:695	a longest linear sequence	671:695	a longest linear sequence of 27 steps in 3% overall yield	671:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	2	12	theme	consecutive	486:496	arg1	α-linkages					498:507	four consecutive α-linkages	481:507	four consecutive α-linkages	481:507	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	3	13	theme	total	630:634	arg1	synthesis					636:644	the total synthesis	626:644	the total synthesis	626:644	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	0	14	theme	Total	0:4	arg1	Synthesis					6:14	Total Synthesis	0:14	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).	0:159	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	1	15	theme	conjugation-ready	190:206	arg1	unit					234:237	the conjugation-ready pentasaccharide repeating unit	186:237	the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1)	186:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	2	16	theme	target	320:325	arg1	pentasaccharide					327:341	The complex target pentasaccharide	308:341	The complex target pentasaccharide	308:341	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	1	17	theme	pentasaccharide	208:222	arg1	unit					234:237	the conjugation-ready pentasaccharide repeating unit	186:237	the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1)	186:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	2	18	theme	complex	312:318	arg1	pentasaccharide					327:341	The complex target pentasaccharide	308:341	The complex target pentasaccharide	308:341	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	1	19	theme	repeating	224:232	arg1	unit					234:237	the conjugation-ready pentasaccharide repeating unit	186:237	the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1)	186:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	0	20	theme	Sugar-Containing	32:47	arg1	Unit					75:78	the All-Rare Sugar-Containing Pentasaccharide Repeating Unit	19:78	the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1)	19:158	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	3	21	theme	steps	703:707	arg1	sequence					688:695	a longest linear sequence	671:695	a longest linear sequence of 27 steps in 3% overall yield	671:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	2	22	theme	functionalized	401:414	arg1	d-bacillosamine					416:430	orthogonally functionalized d-bacillosamine	388:430	orthogonally functionalized d-bacillosamine	388:430	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	1	23	theme	unit	234:237	arg1	synthesis					173:181	First total synthesis	161:181	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1)	161:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	0	24	theme	All-Rare	23:30	arg1	Unit					75:78	the All-Rare Sugar-Containing Pentasaccharide Repeating Unit	19:78	the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1)	19:158	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	0	25	theme	Serotype	147:154	arg1	302-73					139:144	Strain 302-73	132:144	Plesiomonas shigelloides Strain 302-73 (Serotype O1)	107:158	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	0	25	theme	Serotype	147:154	arg1	O1					156:157	Serotype O1	147:157	Serotype O1	147:157	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	3	26	from	steps	703:707	arg1	yield					723:727	3% overall yield	712:727	3% overall yield	712:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	0	27	theme	Repeating	65:73	arg1	Unit					75:78	the All-Rare Sugar-Containing Pentasaccharide Repeating Unit	19:78	the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1)	19:158	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	3	28	theme	l-fucosamine	552:563	arg1	glycosylations					585:598	l-fucosamine and stereoselective glycosylations	552:598	l-fucosamine and stereoselective glycosylations	552:598	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	1	29	dep	shigelloides	254:265	arg1	302-73					274:279	strain 302-73	267:279	Plesiomonas shigelloides strain 302-73 (serotype O1)	242:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	1	29	dep	shigelloides	254:265	arg1	O1					291:292	serotype O1	282:292	serotype O1	282:292	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	2	30	theme	amino	367:371	arg1	l-fucosamine					433:444	l-fucosamine	433:444	l-fucosamine	433:444	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	2	30	theme	amino	367:371	arg1	l-pneumosamine					451:464	l-pneumosamine	451:464	l-pneumosamine	451:464	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	2	30	theme	amino	367:371	arg1	d-bacillosamine					416:430	orthogonally functionalized d-bacillosamine	388:430	orthogonally functionalized d-bacillosamine	388:430	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	2	30	theme	amino	367:371	arg1	sugars					373:378	all-rare amino sugars	358:378	all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages	358:507	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	0	31	theme	Pentasaccharide	49:63	arg1	Unit					75:78	the All-Rare Sugar-Containing Pentasaccharide Repeating Unit	19:78	the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1)	19:158	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	3	32	theme	%	713:713	arg1	yield					723:727	3% overall yield	712:727	3% overall yield	712:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	2	33	theme	all-rare	358:365	arg1	l-fucosamine					433:444	l-fucosamine	433:444	l-fucosamine	433:444	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	2	33	theme	all-rare	358:365	arg1	l-pneumosamine					451:464	l-pneumosamine	451:464	l-pneumosamine	451:464	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	2	33	theme	all-rare	358:365	arg1	d-bacillosamine					416:430	orthogonally functionalized d-bacillosamine	388:430	orthogonally functionalized d-bacillosamine	388:430	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	2	33	theme	all-rare	358:365	arg1	sugars					373:378	all-rare amino sugars	358:378	all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages	358:507	The complex target pentasaccharide is composed of all-rare amino sugars such as orthogonally functionalized d-bacillosamine, l-fucosamine, and l-pneumosamine linked through four consecutive α-linkages.					
34291950	3	34	from	sequence	688:695	arg1	yield					723:727	3% overall yield	712:727	3% overall yield	712:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	3	35	theme	overall	715:721	arg1	yield					723:727	3% overall yield	712:727	3% overall yield	712:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	3	36	theme	longest	673:679	arg1	sequence					688:695	a longest linear sequence	671:695	a longest linear sequence of 27 steps in 3% overall yield	671:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	1	37	theme	Plesiomonas	242:252	arg1	shigelloides					254:265	Plesiomonas shigelloides strain 302-73 (serotype O1)	242:293	Plesiomonas shigelloides strain 302-73 (serotype O1)	242:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	3	38	theme	linear	681:686	arg1	sequence					688:695	a longest linear sequence	671:695	a longest linear sequence of 27 steps in 3% overall yield	671:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	3	39	theme	axial	538:542	arg1	4-OH					544:547	axial 4-OH	538:547	axial 4-OH of l-fucosamine and stereoselective glycosylations	538:598	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	1	40	theme	shigelloides	254:265	arg1	unit					234:237	the conjugation-ready pentasaccharide repeating unit	186:237	the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1)	186:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	3	41	theme	3	712:712	arg1	%					713:713	%	713:713	%	713:713	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	3	42	theme	poor	514:517	arg1	challenges					612:621	the key challenges	604:621	the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield	604:727	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	3	42	theme	poor	514:517	arg1	nucleophilicity					519:533	The poor nucleophilicity	510:533	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations	510:598	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	3	43	theme	stereoselective	569:583	arg1	glycosylations					585:598	l-fucosamine and stereoselective glycosylations	552:598	l-fucosamine and stereoselective glycosylations	552:598	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
34291950	1	44	theme	strain	267:272	arg1	302-73					274:279	strain 302-73	267:279	Plesiomonas shigelloides strain 302-73 (serotype O1)	242:293	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	1	44	theme	strain	267:272	arg1	O1					291:292	serotype O1	282:292	serotype O1	282:292	First total synthesis of the conjugation-ready pentasaccharide repeating unit of Plesiomonas shigelloides strain 302-73 (serotype O1) is reported.					
34291950	0	45	theme	Unit	75:78	arg1	Synthesis					6:14	Total Synthesis	0:14	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).	0:159	Total Synthesis of the All-Rare Sugar-Containing Pentasaccharide Repeating Unit of the O-Polysaccharide of Plesiomonas shigelloides Strain 302-73 (Serotype O1).					
34291950	3	46	theme	glycosylations	585:598	arg1	4-OH					544:547	axial 4-OH	538:547	axial 4-OH of l-fucosamine and stereoselective glycosylations	538:598	The poor nucleophilicity of axial 4-OH of l-fucosamine and stereoselective glycosylations are the key challenges in the total synthesis, which was completed via a longest linear sequence of 27 steps in 3% overall yield.					
33007535	4	0	theme	high-performance	915:930	arg1	chromatography					939:952	high-performance liquid chromatography	915:952	high-performance liquid chromatography	915:952	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	6	1	theme	yellow	1392:1397	arg1	polysaccharide					1403:1416	yellow tea polysaccharide	1392:1416	yellow tea polysaccharide	1392:1416	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	4	2	theme	chromatography	939:952	arg1	analysis					998:1005	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis	911:1005	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis	911:1005	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	1	3	theme	molecular	282:290	arg1	Mw					301:302	Mw	301:302	Mw	301:302	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	1	3	theme	molecular	282:290	arg1	weights					292:298	different molecular weights	272:298	different molecular weights (Mw)	272:303	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	2	4	with	precipitation	419:431	arg1	ethanol					438:444	ethanol	438:444	ethanol at various concentrations of 30%, 50%, and 70%, respectively	438:505	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	5	5	theme	electron	1338:1345	arg1	microscopy					1347:1356	scanning electron microscopy	1329:1356	scanning electron microscopy	1329:1356	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	6	6	theme	highest	1489:1495	arg1	activity					1596:1603	superoxide and hydroxyl radicals scavenging activity	1552:1603	superoxide and hydroxyl radicals scavenging activity	1552:1603	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	6	6	theme	highest	1489:1495	arg1	levels					1497:1502	the highest levels	1485:1502	the highest levels of 2	1485:1507	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	6	6	theme	highest	1489:1495	arg1	radical					1543:1549	2-diphenyl-1-picrylhydrazyl free radical	1510:1549	2-diphenyl-1-picrylhydrazyl free radical	1510:1549	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	1	7	theme	irradiation	164:174	arg1	impact					143:148	the impact	139:148	the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw)	139:303	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	2	8	theme	tea	590:592	arg1	polysaccharide					594:607	yellow tea polysaccharide	583:607	yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U	583:646	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	7	9	theme	important	1751:1759	arg1	property					1761:1768	an important property	1748:1768	an important property for functional foods or medicines	1748:1802	These results suggest that the alteration of the spatial structure of yellow tea polysaccharide can enhance its antioxidant activity which is an important property for functional foods or medicines.					
33007535	7	9	theme	important	1751:1759	arg1	activity					1730:1737	its antioxidant activity	1714:1737	its antioxidant activity which is an important property for functional foods or medicines	1714:1802	These results suggest that the alteration of the spatial structure of yellow tea polysaccharide can enhance its antioxidant activity which is an important property for functional foods or medicines.					
33007535	4	10	theme	yellow	1048:1053	arg1	polysaccharide					1059:1072	native yellow tea polysaccharide	1041:1072	native yellow tea polysaccharide treated with ultrasound	1041:1096	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	2	11	theme	intensity	533:541	arg1	20 kHz					555:560	20 kHz	555:560	20 kHz	555:560	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	2	11	theme	intensity	533:541	arg1	ultrasound					543:552	high intensity ultrasound	528:552	high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U	528:646	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	4	12	theme	partial	1018:1024	arg1	degradation					1026:1036	a partial degradation	1016:1036	a partial degradation of native yellow tea polysaccharide treated with ultrasound	1016:1096	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	3	13	dep	5.2 kDa	736:742	arg1	to					733:734	to	733:734	to	733:734	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	1	14	theme	antioxidant	214:224	arg1	properties					226:235	antioxidant properties	214:235	antioxidant properties	214:235	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	5	15	from	changes	1167:1173	arg1	morphology					1178:1187	morphology	1178:1187	morphology	1178:1187	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	5	15	from	changes	1167:1173	arg1	breakdown					1197:1205	the breakdown	1193:1205	the breakdown of native yellow tea polysaccharide	1193:1241	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	7	16	theme	antioxidant	1718:1728	arg1	property					1761:1768	an important property	1748:1768	an important property for functional foods or medicines	1748:1802	These results suggest that the alteration of the spatial structure of yellow tea polysaccharide can enhance its antioxidant activity which is an important property for functional foods or medicines.					
33007535	7	16	theme	antioxidant	1718:1728	arg1	activity					1730:1737	its antioxidant activity	1714:1737	its antioxidant activity which is an important property for functional foods or medicines	1714:1802	These results suggest that the alteration of the spatial structure of yellow tea polysaccharide can enhance its antioxidant activity which is an important property for functional foods or medicines.					
33007535	6	17	theme	free	1538:1541	arg1	levels					1497:1502	the highest levels	1485:1502	the highest levels of 2	1485:1507	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	6	17	theme	free	1538:1541	arg1	radical					1543:1549	2-diphenyl-1-picrylhydrazyl free radical	1510:1549	2-diphenyl-1-picrylhydrazyl free radical	1510:1549	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	2	18	theme	%	491:491	arg1	concentrations					457:470	various concentrations	449:470	various concentrations of 30%, 50%, and 70%, respectively	449:505	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	4	19	theme	polysaccharide	1059:1072	arg1	degradation					1026:1036	a partial degradation	1016:1036	a partial degradation of native yellow tea polysaccharide treated with ultrasound	1016:1096	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	5	20	theme	yellow	1217:1222	arg1	polysaccharide					1228:1241	native yellow tea polysaccharide	1210:1241	native yellow tea polysaccharide	1210:1241	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	7	21	theme	polysaccharide	1687:1700	arg1	structure					1663:1671	the spatial structure	1651:1671	the spatial structure of yellow tea polysaccharide	1651:1700	These results suggest that the alteration of the spatial structure of yellow tea polysaccharide can enhance its antioxidant activity which is an important property for functional foods or medicines.					
33007535	3	22	dep	15.1 kDa	700:707	arg1	to					697:698	to	697:698	to	697:698	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	1	23	from	impact	143:148	arg1	characteristics					194:208	structural characteristics	183:208	structural characteristics	183:208	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	1	23	from	impact	143:148	arg1	properties					226:235	antioxidant properties	214:235	antioxidant properties	214:235	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	7	24	theme	yellow	1676:1681	arg1	polysaccharide					1687:1700	yellow tea polysaccharide	1676:1700	yellow tea polysaccharide	1676:1700	These results suggest that the alteration of the spatial structure of yellow tea polysaccharide can enhance its antioxidant activity which is an important property for functional foods or medicines.					
33007535	7	25	theme	structure	1663:1671	arg1	alteration					1637:1646	the alteration	1633:1646	the alteration of the spatial structure of yellow tea polysaccharide	1633:1700	These results suggest that the alteration of the spatial structure of yellow tea polysaccharide can enhance its antioxidant activity which is an important property for functional foods or medicines.					
33007535	0	26	theme	tea	104:106	arg1	polysaccharide					108:121	the yellow tea polysaccharide	93:121	the yellow tea polysaccharide	93:121	Ultrasound irradiation alters the spatial structure and improves the antioxidant activity of the yellow tea polysaccharide.					
33007535	3	27	from	15.1 kDa	700:707	arg1	Mw					671:672	Mw	671:672	Mw	671:672	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	27	from	15.1 kDa	700:707	arg1	YTPS-3N					678:684	YTPS-3N	678:684	YTPS-3N (from 37.7 to 15.1 kDa)	678:708	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	27	from	15.1 kDa	700:707	arg1	weight					663:668	The molecular weight	649:668	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa)	649:743	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	27	from	15.1 kDa	700:707	arg1	YTPS-5N					714:720	YTPS-5N	714:720	YTPS-5N (from 14.6 to 5.2 kDa)	714:743	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	0	28	theme	Ultrasound	0:9	arg1	irradiation					11:21	Ultrasound irradiation	0:21	Ultrasound irradiation	0:21	Ultrasound irradiation alters the spatial structure and improves the antioxidant activity of the yellow tea polysaccharide.					
33007535	6	29	theme	radicals	1576:1583	arg1	activity					1596:1603	superoxide and hydroxyl radicals scavenging activity	1552:1603	superoxide and hydroxyl radicals scavenging activity	1552:1603	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	6	29	theme	radicals	1576:1583	arg1	levels					1497:1502	the highest levels	1485:1502	the highest levels of 2	1485:1507	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	5	30	theme	polysaccharide	1228:1241	arg1	morphology					1178:1187	morphology	1178:1187	morphology	1178:1187	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	5	30	theme	polysaccharide	1228:1241	arg1	breakdown					1197:1205	the breakdown	1193:1205	the breakdown of native yellow tea polysaccharide	1193:1241	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	2	31	theme	%	482:482	arg1	concentrations					457:470	various concentrations	449:470	various concentrations of 30%, 50%, and 70%, respectively	449:505	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	0	32	theme	spatial	34:40	arg1	structure					42:50	the spatial structure	30:50	the spatial structure	30:50	Ultrasound irradiation alters the spatial structure and improves the antioxidant activity of the yellow tea polysaccharide.					
33007535	1	33	with	polysaccharides	251:265	arg1	Mw					301:302	Mw	301:302	Mw	301:302	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	1	33	with	polysaccharides	251:265	arg1	weights					292:298	different molecular weights	272:298	different molecular weights (Mw)	272:303	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	4	34	theme	transform-infrared	966:983	arg1	spectroscopy					985:996	Fourier transform-infrared spectroscopy	958:996	Fourier transform-infrared spectroscopy	958:996	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	3	35	from	5.2 kDa	736:742	arg1	Mw					671:672	Mw	671:672	Mw	671:672	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	35	from	5.2 kDa	736:742	arg1	YTPS-3N					678:684	YTPS-3N	678:684	YTPS-3N (from 37.7 to 15.1 kDa)	678:708	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	35	from	5.2 kDa	736:742	arg1	weight					663:668	The molecular weight	649:668	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa)	649:743	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	35	from	5.2 kDa	736:742	arg1	YTPS-5N					714:720	YTPS-5N	714:720	YTPS-5N (from 14.6 to 5.2 kDa)	714:743	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	36	theme	molecular	653:661	arg1	Mw					671:672	Mw	671:672	Mw	671:672	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	36	theme	molecular	653:661	arg1	weight					663:668	The molecular weight	649:668	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa)	649:743	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	5	37	theme	scanning	1329:1336	arg1	microscopy					1347:1356	scanning electron microscopy	1329:1356	scanning electron microscopy	1329:1356	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	2	38	theme	tea	338:340	arg1	polysaccharide					342:355	Native yellow tea polysaccharide	324:355	Native yellow tea polysaccharide	324:355	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	6	39	theme	radical	1433:1439	arg1	scavenging					1441:1450	free radical scavenging	1428:1450	free radical scavenging activity	1428:1459	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	5	40	theme	dichroism	1292:1300	arg1	spectrum					1302:1309	the circular dichroism spectrum	1279:1309	the circular dichroism spectrum	1279:1309	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	1	41	theme	yellow	240:245	arg1	polysaccharides					251:265	yellow tea polysaccharides	240:265	yellow tea polysaccharides with different molecular weights (Mw)	240:303	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	4	42	theme	liquid	932:937	arg1	chromatography					939:952	high-performance liquid chromatography	915:952	high-performance liquid chromatography	915:952	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	6	43	theme	tea	1399:1401	arg1	polysaccharide					1403:1416	yellow tea polysaccharide	1392:1416	yellow tea polysaccharide	1392:1416	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	1	44	theme	polysaccharides	251:265	arg1	characteristics					194:208	structural characteristics	183:208	structural characteristics	183:208	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	1	44	theme	polysaccharides	251:265	arg1	properties					226:235	antioxidant properties	214:235	antioxidant properties	214:235	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	7	45	theme	functional	1774:1783	arg1	foods					1785:1789	functional foods	1774:1789	functional foods	1774:1789	These results suggest that the alteration of the spatial structure of yellow tea polysaccharide can enhance its antioxidant activity which is an important property for functional foods or medicines.					
33007535	1	46	theme	different	272:280	arg1	Mw					301:302	Mw	301:302	Mw	301:302	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	1	46	theme	different	272:280	arg1	weights					292:298	different molecular weights	272:298	different molecular weights (Mw)	272:303	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	4	47	theme	native	1041:1046	arg1	polysaccharide					1059:1072	native yellow tea polysaccharide	1041:1072	native yellow tea polysaccharide treated with ultrasound	1041:1096	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	1	48	theme	ultrasound	153:162	arg1	irradiation					164:174	ultrasound irradiation	153:174	ultrasound irradiation	153:174	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	4	49	theme	tea	1055:1057	arg1	polysaccharide					1059:1072	native yellow tea polysaccharide	1041:1072	native yellow tea polysaccharide treated with ultrasound	1041:1096	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	3	50	theme	YTPS-3N	678:684	arg1	Mw					671:672	Mw	671:672	Mw	671:672	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	50	theme	YTPS-3N	678:684	arg1	weight					663:668	The molecular weight	649:668	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa)	649:743	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	2	51	theme	Native	324:329	arg1	polysaccharide					342:355	Native yellow tea polysaccharide	324:355	Native yellow tea polysaccharide	324:355	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	2	52	theme	yellow	583:588	arg1	polysaccharide					594:607	yellow tea polysaccharide	583:607	yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U	583:646	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	1	53	theme	structural	183:192	arg1	characteristics					194:208	structural characteristics	183:208	structural characteristics	183:208	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	2	54	theme	high	528:531	arg1	20 kHz					555:560	20 kHz	555:560	20 kHz	555:560	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	2	54	theme	high	528:531	arg1	ultrasound					543:552	high intensity ultrasound	528:552	high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U	528:646	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	3	55	from	weight	663:668	arg1	5.2 kDa					736:742	5.2 kDa	736:742	5.2 kDa	736:742	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	55	from	weight	663:668	arg1	15.1 kDa					700:707	15.1 kDa	700:707	15.1 kDa	700:707	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	6	56	theme	superoxide	1552:1561	arg1	activity					1596:1603	superoxide and hydroxyl radicals scavenging activity	1552:1603	superoxide and hydroxyl radicals scavenging activity	1552:1603	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	6	56	theme	superoxide	1552:1561	arg1	levels					1497:1502	the highest levels	1485:1502	the highest levels of 2	1485:1507	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	4	57	theme	monosaccharide	1110:1123	arg1	composition					1125:1135	the monosaccharide composition	1106:1135	the monosaccharide composition	1106:1135	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	3	58	theme	YTPS-5N	714:720	arg1	Mw					671:672	Mw	671:672	Mw	671:672	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	58	theme	YTPS-5N	714:720	arg1	weight					663:668	The molecular weight	649:668	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa)	649:743	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	59	theme	particle	807:814	arg1	Zavg					822:825	Zavg	822:825	Zavg	822:825	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	3	59	theme	particle	807:814	arg1	size					816:819	particle size	807:819	particle size (Zavg)	807:826	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	5	60	theme	native	1210:1215	arg1	polysaccharide					1228:1241	native yellow tea polysaccharide	1210:1241	native yellow tea polysaccharide	1210:1241	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	0	61	theme	antioxidant	69:79	arg1	activity					81:88	the antioxidant activity	65:88	the antioxidant activity of the yellow tea polysaccharide	65:121	Ultrasound irradiation alters the spatial structure and improves the antioxidant activity of the yellow tea polysaccharide.					
33007535	6	62	theme	2-diphenyl-1-picrylhydrazyl	1510:1536	arg1	levels					1497:1502	the highest levels	1485:1502	the highest levels of 2	1485:1507	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	6	62	theme	2-diphenyl-1-picrylhydrazyl	1510:1536	arg1	radical					1543:1549	2-diphenyl-1-picrylhydrazyl free radical	1510:1549	2-diphenyl-1-picrylhydrazyl free radical	1510:1549	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	2	63	with	irradiated	512:521	arg1	20 kHz					555:560	20 kHz	555:560	20 kHz	555:560	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	2	63	with	irradiated	512:521	arg1	ultrasound					543:552	high intensity ultrasound	528:552	high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U	528:646	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	2	64	from	concentrations	457:470	arg1	ethanol					438:444	ethanol	438:444	ethanol at various concentrations of 30%, 50%, and 70%, respectively	438:505	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	6	65	theme	2	1507:1507	arg1	activity					1596:1603	superoxide and hydroxyl radicals scavenging activity	1552:1603	superoxide and hydroxyl radicals scavenging activity	1552:1603	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	6	65	theme	2	1507:1507	arg1	levels					1497:1502	the highest levels	1485:1502	the highest levels of 2	1485:1507	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	6	65	theme	2	1507:1507	arg1	radical					1543:1549	2-diphenyl-1-picrylhydrazyl free radical	1510:1549	2-diphenyl-1-picrylhydrazyl free radical	1510:1549	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	3	66	theme	ultrasound	768:777	arg1	irradiation					779:789	ultrasound irradiation	768:789	ultrasound irradiation	768:789	The molecular weight (Mw) of YTPS-3N (from 37.7 to 15.1 kDa) and YTPS-5N (from 14.6 to 5.2 kDa) sharply decreased upon ultrasound irradiation, coincidentally particle size (Zavg) was also significantly reduced for YTPS-3N (40%), YTPS-5N (48%) and YTPS-7N (54%).					
33007535	0	67	theme	yellow	97:102	arg1	polysaccharide					108:121	the yellow tea polysaccharide	93:121	the yellow tea polysaccharide	93:121	Ultrasound irradiation alters the spatial structure and improves the antioxidant activity of the yellow tea polysaccharide.					
33007535	4	68	dep	chromatography	939:952	arg1	The					911:913	The	911:913	The	911:913	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	7	69	theme	tea	1683:1685	arg1	polysaccharide					1687:1700	yellow tea polysaccharide	1676:1700	yellow tea polysaccharide	1676:1700	These results suggest that the alteration of the spatial structure of yellow tea polysaccharide can enhance its antioxidant activity which is an important property for functional foods or medicines.					
33007535	0	70	theme	polysaccharide	108:121	arg1	activity					81:88	the antioxidant activity	65:88	the antioxidant activity of the yellow tea polysaccharide	65:121	Ultrasound irradiation alters the spatial structure and improves the antioxidant activity of the yellow tea polysaccharide.					
33007535	6	71	theme	scavenging	1585:1594	arg1	activity					1596:1603	superoxide and hydroxyl radicals scavenging activity	1552:1603	superoxide and hydroxyl radicals scavenging activity	1552:1603	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	6	71	theme	scavenging	1585:1594	arg1	levels					1497:1502	the highest levels	1485:1502	the highest levels of 2	1485:1507	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	7	72	theme	spatial	1655:1661	arg1	structure					1663:1671	the spatial structure	1651:1671	the spatial structure of yellow tea polysaccharide	1651:1700	These results suggest that the alteration of the spatial structure of yellow tea polysaccharide can enhance its antioxidant activity which is an important property for functional foods or medicines.					
33007535	5	73	theme	tea	1224:1226	arg1	polysaccharide					1228:1241	native yellow tea polysaccharide	1210:1241	native yellow tea polysaccharide	1210:1241	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	6	74	theme	hydroxyl	1567:1574	arg1	radicals					1576:1583	hydroxyl radicals	1567:1583	hydroxyl radicals	1567:1583	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	2	75	theme	various	449:455	arg1	concentrations					457:470	various concentrations	449:470	various concentrations of 30%, 50%, and 70%, respectively	449:505	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	5	76	theme	atomic	1312:1317	arg1	force					1319:1323	atomic force	1312:1323	atomic force	1312:1323	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	2	77	theme	%	477:477	arg1	concentrations					457:470	various concentrations	449:470	various concentrations of 30%, 50%, and 70%, respectively	449:505	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	6	78	theme	scavenging	1441:1450	arg1	consequence					1364:1374	a consequence	1362:1374	a consequence	1362:1374	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	6	78	theme	scavenging	1441:1450	arg1	activity					1452:1459	free radical scavenging activity	1428:1459	free radical scavenging activity	1428:1459	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	4	79	theme	Fourier	958:964	arg1	spectroscopy					985:996	Fourier transform-infrared spectroscopy	958:996	Fourier transform-infrared spectroscopy	958:996	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	5	80	theme	circular	1283:1290	arg1	spectrum					1302:1309	the circular dichroism spectrum	1279:1309	the circular dichroism spectrum	1279:1309	Furthermore, changes in morphology and the breakdown of native yellow tea polysaccharide upon irradiation was confirmed with the circular dichroism spectrum, atomic force and scanning electron microscopy.					
33007535	2	81	theme	yellow	331:336	arg1	polysaccharide					342:355	Native yellow tea polysaccharide	324:355	Native yellow tea polysaccharide	324:355	Native yellow tea polysaccharide containing YTPS-3N, YTPS-5N and YTPS-7N were prepared through precipitation with ethanol at various concentrations of 30%, 50%, and 70%, respectively, and irradiated with high intensity ultrasound (20 kHz) for 55 min to yield yellow tea polysaccharide including YTPS-3U, YTPS-5U and YTPS-7U.					
33007535	6	82	theme	free	1428:1431	arg1	scavenging					1441:1450	free radical scavenging	1428:1450	free radical scavenging activity	1428:1459	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	4	83	theme	spectroscopy	985:996	arg1	analysis					998:1005	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis	911:1005	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis	911:1005	The high-performance liquid chromatography and Fourier transform-infrared spectroscopy analysis revealed a partial degradation of native yellow tea polysaccharide treated with ultrasound, though the monosaccharide composition was not altered.					
33007535	1	84	dep	characteristics	194:208	arg1	the					179:181	the	179:181	the	179:181	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
33007535	6	85	theme	polysaccharide	1403:1416	arg1	irradiation					1377:1387	irradiation	1377:1387	irradiation of yellow tea polysaccharide	1377:1416	As a consequence, irradiation of yellow tea polysaccharide increased free radical scavenging activity with YTPS-7U exhibiting the highest levels of 2, 2-diphenyl-1-picrylhydrazyl free radical, superoxide and hydroxyl radicals scavenging activity.					
33007535	1	86	theme	tea	247:249	arg1	polysaccharides					251:265	yellow tea polysaccharides	240:265	yellow tea polysaccharides with different molecular weights (Mw)	240:303	In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated.					
32437801	0	0	theme	inhibitory	86:95	arg1	effects					97:103	and their inhibitory effects	76:103	effects	97:103	Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function.					
32437801	2	1	theme	acidic	475:480	arg1	fractions					482:490	two acidic fractions	471:490	two acidic fractions (WCPP-A1 and WCPP-A2)	471:512	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	1	theme	acidic	475:480	arg1	WCPP-A2					505:511	WCPP-A2	505:511	WCPP-A2	505:511	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	1	theme	acidic	475:480	arg1	WCPP-A1					493:499	WCPP-A1	493:499	WCPP-A1	493:499	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	2	theme	homogeneous	586:596	arg1	WCPP-A2b					626:633	WCPP-A2b	626:633	WCPP-A2b	626:633	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	2	theme	homogeneous	586:596	arg1	WCPP-A2a					613:620	WCPP-A2a	613:620	WCPP-A2a	613:620	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	2	theme	homogeneous	586:596	arg1	sub-fractions					598:610	two homogeneous sub-fractions	582:610	two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b)	582:634	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	1	3	theme	dietary	156:162	arg1	Pollen					129:134	Pollen	129:134	Pollen	129:134	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
32437801	1	3	theme	dietary	156:162	arg1	supplement					164:173	dietary supplement	156:173	dietary supplement used to supplement the diet in many countries	156:219	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
32437801	5	4	theme	strongest	1400:1408	arg1	effect					1421:1426	the strongest inhibitory effect	1396:1426	the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL	1396:1473	The results of galectin-3-mediated hemagglutination assay indicated that WCPP-A2a exhibited the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL.					
32437801	3	5	theme	composition	678:688	arg1	results					690:696	Monosaccharide composition results	663:696	Monosaccharide composition results	663:696	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	4	6	theme	type-II	1245:1251	arg1	chain					1274:1278	type-II arabinogalactan side chain	1245:1278	α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached	1211:1301	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	2	7	theme	Sepharose	639:647	arg1	column					655:660	Sepharose CL-6B column	639:660	Sepharose CL-6B column	639:660	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	8	theme	water-soluble	339:351	arg1	polysaccharide					353:366	the water-soluble polysaccharide	335:366	the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP)	335:413	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	4	9	theme	NMR	1068:1070	arg1	analysis					1080:1087	NMR spectra analysis	1068:1087	NMR spectra analysis	1068:1087	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	0	10	from	Analysis	0:7	arg1	Camellia					51:58	Camellia	51:58	Camellia	51:58	Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function.					
32437801	5	11	theme	MIC	1447:1449	arg1	value					1451:1455	MIC value	1447:1455	MIC value around 0.27 μg/mL	1447:1473	The results of galectin-3-mediated hemagglutination assay indicated that WCPP-A2a exhibited the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL.					
32437801	4	12	from	O-4	1169:1171	arg1	backbone					1196:1203	the backbone	1192:1203	the backbone	1192:1203	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	6	13	theme	Camellia	1521:1528	arg1	polysaccharide					1546:1559	Camellia japonica pollen polysaccharide	1521:1559	Camellia japonica pollen polysaccharide	1521:1559	These results suggested the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods.					
32437801	3	14	theme	domain	910:915	arg1	backbone					917:924	rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone	860:924	rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan	860:1019	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	5	15	theme	galectin-3-mediated	1319:1337	arg1	assay					1356:1360	galectin-3-mediated hemagglutination assay	1319:1360	galectin-3-mediated hemagglutination assay	1319:1360	The results of galectin-3-mediated hemagglutination assay indicated that WCPP-A2a exhibited the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL.					
32437801	0	16	from	Camellia	51:58	arg1	Analysis					0:7	Analysis	0:7	Analysis of the water-soluble polysaccharides from Camellia japonica	0:67	Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function.					
32437801	0	16	from	Camellia	51:58	arg1	polysaccharides					30:44	the water-soluble polysaccharides	12:44	the water-soluble polysaccharides from Camellia japonica	12:67	Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function.					
32437801	4	17	theme	pectic	1130:1135	arg1	polysaccharide					1137:1150	an RG-I-like pectic polysaccharide	1117:1150	an RG-I-like pectic polysaccharide	1117:1150	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	4	17	theme	pectic	1130:1135	arg1	WCPP-A2a					1104:1111	WCPP-A2a	1104:1111	WCPP-A2a	1104:1111	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	0	18	theme	galectin-3	108:117	arg1	function					119:126	galectin-3 function	108:126	galectin-3 function	108:126	Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function.					
32437801	5	19	theme	inhibitory	1410:1419	arg1	effect					1421:1426	the strongest inhibitory effect	1396:1426	the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL	1396:1473	The results of galectin-3-mediated hemagglutination assay indicated that WCPP-A2a exhibited the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL.					
32437801	2	20	dep	sub-fractions	598:610	arg1	WCPP-A2b					626:633	WCPP-A2b	626:633	WCPP-A2b	626:633	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	20	dep	sub-fractions	598:610	arg1	WCPP-A2a					613:620	WCPP-A2a	613:620	WCPP-A2a	613:620	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	20	dep	sub-fractions	598:610	arg1	sub-fractions					598:610	two homogeneous sub-fractions	582:610	two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b)	582:634	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	3	21	theme	pectic	888:893	arg1	backbone					917:924	rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone	860:924	rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan	860:1019	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	6	22	theme	functional	1589:1598	arg1	foods					1600:1604	functional foods	1589:1604	functional foods	1589:1604	These results suggested the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods.					
32437801	4	23	theme	primary	1026:1032	arg1	analysis					1044:1051	The primary structure analysis	1022:1051	The primary structure analysis of WCPP-A2a by NMR spectra analysis	1022:1087	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	5	24	with	effect	1421:1426	arg1	value					1451:1455	MIC value	1447:1455	MIC value around 0.27 μg/mL	1447:1473	The results of galectin-3-mediated hemagglutination assay indicated that WCPP-A2a exhibited the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL.					
32437801	3	25	theme	polysaccharide	895:908	arg1	backbone					917:924	rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone	860:924	rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan	860:1019	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	6	26	theme	polysaccharide	1546:1559	arg1	use					1514:1516	the potential use	1500:1516	the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods	1500:1604	These results suggested the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods.					
32437801	2	27	theme	neutral	441:447	arg1	WCPP-N					459:464	WCPP-N	459:464	WCPP-N	459:464	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	27	theme	neutral	441:447	arg1	fraction					449:456	one neutral fraction	437:456	one neutral fraction (WCPP-N)	437:465	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	1	28	theme	Camellia	264:271	arg1	polysaccharide					289:302	Camellia japonica pollen polysaccharide	264:302	Camellia japonica pollen polysaccharide	264:302	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
32437801	3	29	with	backbone	917:924	arg1	arabinogalactan					1005:1019	arabinogalactan	1005:1019	arabinogalactan	1005:1019	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	29	with	backbone	917:924	arg1	arabinan					978:985	arabinan	978:985	arabinan	978:985	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	29	with	backbone	917:924	arg1	galactan					988:995	galactan	988:995	galactan	988:995	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	29	with	backbone	917:924	arg1	types					946:950	some different types	931:950	some different types	931:950	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	4	30	theme	α-	1211:1212	arg1	-L-arabinan					1222:1232	α-(1 → 3,5)-L-arabinan	1211:1232	α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached	1211:1301	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	3	31	theme	different	936:944	arg1	arabinogalactan					1005:1019	arabinogalactan	1005:1019	arabinogalactan	1005:1019	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	31	theme	different	936:944	arg1	arabinan					978:985	arabinan	978:985	arabinan	978:985	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	31	theme	different	936:944	arg1	galactan					988:995	galactan	988:995	galactan	988:995	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	31	theme	different	936:944	arg1	types					946:950	some different types	931:950	some different types	931:950	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	2	32	theme	Camellia	383:390	arg1	japonica					392:399	Camellia japonica pollen	383:406	Camellia japonica pollen (WCPP)	383:413	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	32	theme	Camellia	383:390	arg1	WCPP					409:412	WCPP	409:412	WCPP	409:412	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	0	33	theme	water-soluble	16:28	arg1	polysaccharides					30:44	the water-soluble polysaccharides	12:44	the water-soluble polysaccharides from Camellia japonica	12:67	Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function.					
32437801	1	34	theme	japonica	273:280	arg1	polysaccharide					289:302	Camellia japonica pollen polysaccharide	264:302	Camellia japonica pollen polysaccharide	264:302	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
32437801	2	35	theme	CL-6B	649:653	arg1	column					655:660	Sepharose CL-6B column	639:660	Sepharose CL-6B column	639:660	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	4	36	theme	1 → 3,5	1214:1220	arg1	-L-arabinan					1222:1232	α-(1 → 3,5)-L-arabinan	1211:1232	α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached	1211:1301	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	4	37	theme	structure	1034:1042	arg1	analysis					1044:1051	The primary structure analysis	1022:1051	The primary structure analysis of WCPP-A2a by NMR spectra analysis	1022:1087	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	1	38	theme	pollen	282:287	arg1	polysaccharide					289:302	Camellia japonica pollen polysaccharide	264:302	Camellia japonica pollen polysaccharide	264:302	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
32437801	4	39	theme	side	1269:1272	arg1	chain					1274:1278	type-II arabinogalactan side chain	1245:1278	α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached	1211:1301	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	4	40	from	backbone	1196:1203	arg1	O-4					1169:1171	the O-4	1165:1171	the O-4 of Rha residues in the backbone	1165:1203	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	4	41	theme	RG-I-like	1120:1128	arg1	polysaccharide					1137:1150	an RG-I-like pectic polysaccharide	1117:1150	an RG-I-like pectic polysaccharide	1117:1150	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	4	41	theme	RG-I-like	1120:1128	arg1	WCPP-A2a					1104:1111	WCPP-A2a	1104:1111	WCPP-A2a	1104:1111	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	6	42	theme	pollen	1539:1544	arg1	polysaccharide					1546:1559	Camellia japonica pollen polysaccharide	1521:1559	Camellia japonica pollen polysaccharide	1521:1559	These results suggested the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods.					
32437801	1	43	theme	polysaccharide	289:302	arg1	activity					252:259	activity	252:259	activity	252:259	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
32437801	1	43	theme	polysaccharide	289:302	arg1	structure					238:246	primary structure	230:246	primary structure	230:246	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
32437801	2	44	theme	DEAE-cellulose	517:530	arg1	column					532:537	DEAE-cellulose column	517:537	DEAE-cellulose column	517:537	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	4	45	theme	arabinogalactan	1253:1267	arg1	chain					1274:1278	type-II arabinogalactan side chain	1245:1278	α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached	1211:1301	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	1	46	theme	many	206:209	arg1	countries					211:219	many countries	206:219	many countries	206:219	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
32437801	0	47	theme	polysaccharides	30:44	arg1	Analysis					0:7	Analysis	0:7	Analysis of the water-soluble polysaccharides from Camellia japonica	0:67	Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function.					
32437801	0	48	dep	Camellia	51:58	arg1	japonica					60:67	Camellia japonica	51:67	Camellia japonica	51:67	Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function.					
32437801	3	49	theme	chains	960:965	arg1	arabinogalactan					1005:1019	arabinogalactan	1005:1019	arabinogalactan	1005:1019	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	49	theme	chains	960:965	arg1	arabinan					978:985	arabinan	978:985	arabinan	978:985	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	49	theme	chains	960:965	arg1	galactan					988:995	galactan	988:995	galactan	988:995	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	49	theme	chains	960:965	arg1	types					946:950	some different types	931:950	some different types	931:950	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	4	50	from	residues	1180:1187	arg1	backbone					1196:1203	the backbone	1192:1203	the backbone	1192:1203	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	4	51	theme	Rha	1176:1178	arg1	residues					1180:1187	Rha residues	1176:1187	Rha residues in the backbone	1176:1203	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	1	52	from	diet	198:201	arg1	countries					211:219	many countries	206:219	many countries	206:219	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
32437801	6	53	theme	galectin3	1566:1574	arg1	inhibitor					1576:1584	a galectin3 inhibitor	1564:1584	a galectin3 inhibitor in functional foods	1564:1604	These results suggested the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods.					
32437801	3	54	theme	Monosaccharide	663:676	arg1	composition					678:688	Monosaccharide composition	663:688	Monosaccharide composition results	663:696	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	55	theme	side	955:958	arg1	chains					960:965	side chains	955:965	side chains	955:965	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	56	contain	contain	730:736	arg2	arabinogalactan					773:787	some arabinogalactan	768:787	starch-like glucan as well as some arabinogalactan	738:787	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	56	contain	contain	730:736	arg2	glucan					750:755	starch-like glucan	738:755	starch-like glucan as well as some arabinogalactan	738:787	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	3	56	contain	contain	730:736	arg1	WCPP-N					710:715	WCPP-N	710:715	WCPP-N	710:715	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	4	57	theme	spectra	1072:1078	arg1	analysis					1080:1087	NMR spectra analysis	1068:1087	NMR spectra analysis	1068:1087	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	4	58	with	polysaccharide	1137:1150	arg1	chain					1274:1278	type-II arabinogalactan side chain	1245:1278	α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached	1211:1301	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	4	58	with	polysaccharide	1137:1150	arg1	-L-arabinan					1222:1232	α-(1 → 3,5)-L-arabinan	1211:1232	α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached	1211:1301	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	2	59	dep	fractions	482:490	arg1	fractions					482:490	two acidic fractions	471:490	two acidic fractions (WCPP-A1 and WCPP-A2)	471:512	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	59	dep	fractions	482:490	arg1	WCPP-A2					505:511	WCPP-A2	505:511	WCPP-A2	505:511	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	2	59	dep	fractions	482:490	arg1	WCPP-A1					493:499	WCPP-A1	493:499	WCPP-A1	493:499	In this study, the water-soluble polysaccharide extracted from Camellia japonica pollen (WCPP) was fractionated into one neutral fraction (WCPP-N) and two acidic fractions (WCPP-A1 and WCPP-A2) by DEAE-cellulose column, and WCPP-A2 was further fractionated into two homogeneous sub-fractions (WCPP-A2a and WCPP-A2b) by Sepharose CL-6B column.					
32437801	1	60	dep	structure	238:246	arg1	the					226:228	the	226:228	the	226:228	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
32437801	4	61	theme	residues	1180:1187	arg1	O-4					1169:1171	the O-4	1165:1171	the O-4 of Rha residues in the backbone	1165:1203	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	5	62	theme	hemagglutination	1339:1354	arg1	assay					1356:1360	galectin-3-mediated hemagglutination assay	1319:1360	galectin-3-mediated hemagglutination assay	1319:1360	The results of galectin-3-mediated hemagglutination assay indicated that WCPP-A2a exhibited the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL.					
32437801	5	63	from	effect	1421:1426	arg1	galectin-3					1431:1440	galectin-3	1431:1440	galectin-3	1431:1440	The results of galectin-3-mediated hemagglutination assay indicated that WCPP-A2a exhibited the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL.					
32437801	3	64	theme	starch-like	738:748	arg1	glucan					750:755	starch-like glucan	738:755	starch-like glucan as well as some arabinogalactan	738:787	Monosaccharide composition results showed that WCPP-N might mainly contain starch-like glucan as well as some arabinogalactan, while WCPP-A1, WCPP-A2 and its sub-fractions might mainly composed of rhamnogalacturonan I (RG-I) pectic polysaccharide domain backbone with some different types of side chains, including arabinan, galactan, and/or arabinogalactan.					
32437801	0	65	from	effects	97:103	arg1	function					119:126	galectin-3 function	108:126	galectin-3 function	108:126	Analysis of the water-soluble polysaccharides from Camellia japonica pollen and their inhibitory effects on galectin-3 function.					
32437801	5	66	theme	assay	1356:1360	arg1	results					1308:1314	The results	1304:1314	The results of galectin-3-mediated hemagglutination assay	1304:1360	The results of galectin-3-mediated hemagglutination assay indicated that WCPP-A2a exhibited the strongest inhibitory effect on galectin-3 with MIC value around 0.27 μg/mL.					
32437801	6	67	theme	japonica	1530:1537	arg1	polysaccharide					1546:1559	Camellia japonica pollen polysaccharide	1521:1559	Camellia japonica pollen polysaccharide	1521:1559	These results suggested the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods.					
32437801	6	68	theme	potential	1504:1512	arg1	use					1514:1516	the potential use	1500:1516	the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods	1500:1604	These results suggested the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods.					
32437801	6	69	from	inhibitor	1576:1584	arg1	foods					1600:1604	functional foods	1589:1604	functional foods	1589:1604	These results suggested the potential use of Camellia japonica pollen polysaccharide as a galectin3 inhibitor in functional foods.					
32437801	4	70	theme	WCPP-A2a	1056:1063	arg1	analysis					1044:1051	The primary structure analysis	1022:1051	The primary structure analysis of WCPP-A2a by NMR spectra analysis	1022:1087	The primary structure analysis of WCPP-A2a by NMR spectra analysis suggested that WCPP-A2a was an RG-I-like pectic polysaccharide, branched at the O-4 of Rha residues in the backbone, with α-(1 → 3,5)-L-arabinan as well as type-II arabinogalactan side chain to which were attached.					
32437801	1	71	theme	primary	230:236	arg1	structure					238:246	primary structure	230:246	primary structure	230:246	Pollen has been defined as dietary supplement used to supplement the diet in many countries, but the primary structure and activity of Camellia japonica pollen polysaccharide remain unclear.					
33773573	5	0	dep	purified	875:882	arg1	deproteinated					885:897	deproteinated	885:897	deproteinated	885:897	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	2	1	theme	biomass	404:410	arg1	amount					379:384	a significant amount	365:384	a significant amount of cell wall-less biomass	365:410	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	2	1	theme	biomass	404:410	arg1	biomass					404:410	cell wall-less biomass	389:410	cell wall-less biomass	389:410	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	2	2	theme	growth	342:347	arg1	microplasmodia					306:319	the microplasmodia	302:319	the microplasmodia	302:319	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	2	2	theme	growth	342:347	arg1	capable					325:331	capable	325:331	capable	325:331	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	4	3	theme	several	780:786	arg1	lines					800:804	several cancer cell lines	780:804	several cancer cell lines	780:804	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	4	4	theme	microplasmodia	717:730	arg1	EPS					653:655	EPS	653:655	EPS	653:655	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	4	4	theme	microplasmodia	717:730	arg1	exopolysaccharides					633:650	the exopolysaccharides	629:650	the exopolysaccharides (EPS)	629:656	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	4	4	theme	microplasmodia	717:730	arg1	polysaccharides					676:690	intracellular polysaccharides	662:690	intracellular polysaccharides (IPS)	662:696	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	4	4	theme	microplasmodia	717:730	arg1	IPS					693:695	IPS	693:695	IPS	693:695	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	13	5	theme	monosaccharide	2261:2274	arg1	composition					2276:2286	monosaccharide composition	2261:2286	monosaccharide composition	2261:2286	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	12	6	theme	Hela	2066:2069	arg1	cells					2071:2075	Hela cells	2066:2075	Hela cells	2066:2075	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	11	7	theme	crude	1755:1759	arg1	sample					1765:1770	the crude IPS sample	1751:1770	the crude IPS sample (e.g. proteins, peptides or ion metals)	1751:1810	This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component.					
33773573	12	8	theme	cycle	1920:1924	arg1	analysis					1926:1933	Cell cycle analysis	1915:1933	Cell cycle analysis by flow cytometry	1915:1951	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	8	9	theme	purified	1233:1240	arg1	IPS					1242:1244	purified IPS	1233:1244	purified IPS	1233:1244	Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells.					
33773573	10	10	dep	%	1527:1527	arg1	34.76 ± 6.58					1515:1526	34.76 ± 6.58	1515:1526	34.76 ± 6.58	1515:1526	However, although only 34.76 ± 6.58% MCF-7 cells were viable when exposed to the crude IPS, but the partially purified IPS displayed non-toxicity to MCF-7 cells.					
33773573	13	11	theme	sulfate	2237:2243	arg1	contents					2251:2258	total sulfate group contents	2231:2258	total sulfate group contents	2231:2258	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	9	12	theme	purified	1468:1475	arg1	IPS					1477:1479	the partially purified IPS	1454:1479	the partially purified IPS (1 mg/ml)	1454:1489	Only 38.42 ± 2.84% Hela cells remained viable when treated with the partially purified IPS (1 mg/ml).					
33773573	9	12	theme	purified	1468:1475	arg1	1 mg/ml					1482:1488	1 mg/ml	1482:1488	1 mg/ml	1482:1488	Only 38.42 ± 2.84% Hela cells remained viable when treated with the partially purified IPS (1 mg/ml).					
33773573	4	13	theme	P.	701:702	arg1	microplasmodia					717:730	P. polycephalum microplasmodia	701:730	P. polycephalum microplasmodia	701:730	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	10	14	dep	cells	1535:1539	arg1	%					1527:1527	%	1527:1527	%	1527:1527	However, although only 34.76 ± 6.58% MCF-7 cells were viable when exposed to the crude IPS, but the partially purified IPS displayed non-toxicity to MCF-7 cells.					
33773573	5	15	theme	purified	875:882	arg1	EPS					900:902	partially purified (deproteinated) EPS	865:902	partially purified (deproteinated) EPS (2.95 ± 0.85 g/l)	865:920	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	5	15	theme	purified	875:882	arg1	2.95 ± 0.85 g/l					905:919	2.95 ± 0.85 g/l	905:919	2.95 ± 0.85 g/l	905:919	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	5	16	dep	RESULTS	807:813	arg1	yields					819:824	The yields	815:824	The yields of the crude EPS (4.43 ± 0.44 g/l)	815:859	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	12	17	theme	crude	2005:2009	arg1	IPS					2011:2013	the crude IPS	2001:2013	the crude IPS	2001:2013	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	2	18	theme	slime	281:285	arg1	mold					287:290	slime mold	281:290	slime mold	281:290	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	5	19	theme	respective	958:967	arg1	3.46 ± 0.36 g/l					980:994	3.46 ± 0.36 g/l	980:994	3.46 ± 0.36 g/l	980:994	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	5	19	theme	respective	958:967	arg1	IPS					975:977	the respective crude IPS	954:977	the respective crude IPS (3.46 ± 0.36 g/l)	954:995	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	9	20	dep	%	1407:1407	arg1	38.42 ± 2.84					1395:1406	38.42 ± 2.84	1395:1406	38.42 ± 2.84	1395:1406	Only 38.42 ± 2.84% Hela cells remained viable when treated with the partially purified IPS (1 mg/ml).					
33773573	4	21	theme	cell	795:798	arg1	lines					800:804	several cancer cell lines	780:804	several cancer cell lines	780:804	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	8	22	theme	Hela	1302:1305	arg1	none					1349:1352	none	1349:1352	none	1349:1352	Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells.					
33773573	8	22	theme	Hela	1302:1305	arg1	sample					1338:1343	the purified sample	1325:1343	the purified sample	1325:1343	Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells.					
33773573	8	22	theme	Hela	1302:1305	arg1	cells					1307:1311	Hela cells	1302:1311	Hela cells	1302:1311	Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells.					
33773573	0	23	theme	intracellular	79:91	arg1	polysaccharides					93:107	intracellular polysaccharides	79:107	intracellular polysaccharides	79:107	Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia.					
33773573	13	24	theme	chemical	2175:2182	arg1	properties					2184:2193	different chemical properties	2165:2193	different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights	2165:2308	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	24	theme	chemical	2175:2182	arg1	composition					2276:2286	monosaccharide composition	2261:2286	monosaccharide composition	2261:2286	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	24	theme	chemical	2175:2182	arg1	protein					2219:2225	protein	2219:2225	protein	2219:2225	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	24	theme	chemical	2175:2182	arg1	weights					2302:2308	molecular weights	2292:2308	molecular weights	2292:2308	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	24	theme	chemical	2175:2182	arg1	carbohydrate					2205:2216	carbohydrate	2205:2216	carbohydrate	2205:2216	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	6	25	theme	average	1047:1053	arg1	14,762 kDa					1096:1105	14,762 kDa	1096:1105	14,762 kDa	1096:1105	The average molecular weight of the EPS and IPS were 14,762 kDa and 1788 kDa.					
33773573	6	25	theme	average	1047:1053	arg1	weight					1065:1070	The average molecular weight	1043:1070	The average molecular weight of the EPS and IPS	1043:1089	The average molecular weight of the EPS and IPS were 14,762 kDa and 1788 kDa.					
33773573	13	26	dep	had	2161:2163	arg1	led					2317:2319	led	2317:2319	led to different cytotoxicity activities	2317:2356	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	5	27	theme	crude	833:837	arg1	4.43 ± 0.44 g/l					844:858	4.43 ± 0.44 g/l	844:858	4.43 ± 0.44 g/l	844:858	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	5	27	theme	crude	833:837	arg1	EPS					839:841	the crude EPS	829:841	the crude EPS (4.43 ± 0.44 g/l)	829:859	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	1	28	theme	remarkable	217:226	arg1	bioactivities					228:240	remarkable bioactivities	217:240	remarkable bioactivities	217:240	BACKGROUND Microbial polysaccharides have been reported to possess remarkable bioactivities.					
33773573	5	29	theme	purified	1011:1018	arg1	2.45 ± 0.36 g/l					1025:1039	2.45 ± 0.36 g/l	1025:1039	2.45 ± 0.36 g/l	1025:1039	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	5	29	theme	purified	1011:1018	arg1	IPS					1020:1022	partially purified IPS	1001:1022	partially purified IPS (2.45 ± 0.36 g/l)	1001:1040	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	8	30	theme	higher	1275:1280	arg1	cytotoxicity					1282:1293	significantly higher cytotoxicity	1261:1293	significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs	1261:1364	Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells.					
33773573	10	31	theme	purified	1602:1609	arg1	IPS					1611:1613	but the partially purified IPS	1584:1613	IPS	1611:1613	However, although only 34.76 ± 6.58% MCF-7 cells were viable when exposed to the crude IPS, but the partially purified IPS displayed non-toxicity to MCF-7 cells.					
33773573	3	32	theme	limited	430:436	arg1	understanding					438:450	a limited understanding	428:450	a limited understanding of the polysaccharides produced by microplasmodia of slime molds	428:515	There has been a limited understanding of the polysaccharides produced by microplasmodia of slime molds, including P. polycephalum.					
33773573	14	33	theme	potential	2406:2414	arg1	materials					2416:2424	potential materials	2406:2424	potential materials for further study relating to cancer treatment	2406:2471	The crude and partially purified IPSs would be potential materials for further study relating to cancer treatment.					
33773573	14	33	theme	potential	2406:2414	arg1	IPSs					2392:2395	The crude and partially purified IPSs	2359:2395	The crude and partially purified IPSs	2359:2395	The crude and partially purified IPSs would be potential materials for further study relating to cancer treatment.					
33773573	11	34	theme	purification	1820:1831	arg1	process					1833:1839	the purification process	1816:1839	the purification process	1816:1839	This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component.					
33773573	0	35	theme	Cytotoxicity	0:11	arg1	activities					13:22	Cytotoxicity activities	0:22	Cytotoxicity activities	0:22	Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia.					
33773573	7	36	dep	glucose	1200:1206	arg1	%					1214:1214	84.46%	1209:1214	84.46%	1209:1214	The major monomer of the EPS was galactose (80.22%), while that of the IPS was glucose (84.46%).					
33773573	13	37	theme	microplasmodia	2146:2159	arg1	IPS					2123:2125	IPS	2123:2125	IPS	2123:2125	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	37	theme	microplasmodia	2146:2159	arg1	EPS					2115:2117	EPS	2115:2117	EPS	2115:2117	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	37	theme	microplasmodia	2146:2159	arg1	CONCLUSIONS					2099:2109	CONCLUSIONS	2099:2109	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia	2099:2159	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	1	38	theme	BACKGROUND	150:159	arg1	polysaccharides					171:185	BACKGROUND Microbial polysaccharides	150:185	BACKGROUND Microbial polysaccharides	150:185	BACKGROUND Microbial polysaccharides have been reported to possess remarkable bioactivities.					
33773573	3	39	theme	polysaccharides	459:473	arg1	understanding					438:450	a limited understanding	428:450	a limited understanding of the polysaccharides produced by microplasmodia of slime molds	428:515	There has been a limited understanding of the polysaccharides produced by microplasmodia of slime molds, including P. polycephalum.					
33773573	2	40	theme	Physarum	243:250	arg1	polycephalum					252:263	Physarum polycephalum	243:263	Physarum polycephalum	243:263	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	2	40	theme	Physarum	243:250	arg1	species					270:276	a species	268:276	a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass	268:410	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	3	41	theme	molds	511:515	arg1	microplasmodia					487:500	microplasmodia	487:500	microplasmodia of slime molds	487:515	There has been a limited understanding of the polysaccharides produced by microplasmodia of slime molds, including P. polycephalum.					
33773573	14	42	theme	purified	2383:2390	arg1	materials					2416:2424	potential materials	2406:2424	potential materials for further study relating to cancer treatment	2406:2471	The crude and partially purified IPSs would be potential materials for further study relating to cancer treatment.					
33773573	14	42	theme	purified	2383:2390	arg1	IPSs					2392:2395	The crude and partially purified IPSs	2359:2395	The crude and partially purified IPSs	2359:2395	The crude and partially purified IPSs would be potential materials for further study relating to cancer treatment.					
33773573	2	43	theme	wall-less	394:402	arg1	biomass					404:410	cell wall-less biomass	389:410	cell wall-less biomass	389:410	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	0	44	theme	exopolysaccharides	56:73	arg1	activities					13:22	Cytotoxicity activities	0:22	Cytotoxicity activities	0:22	Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia.					
33773573	0	44	theme	exopolysaccharides	56:73	arg1	characteristics					37:51	chemical characteristics	28:51	chemical characteristics	28:51	Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia.					
33773573	13	45	theme	cytotoxicity	2334:2345	arg1	activities					2347:2356	different cytotoxicity activities	2324:2356	different cytotoxicity activities	2324:2356	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	14	46	theme	cancer	2456:2461	arg1	treatment					2463:2471	cancer treatment	2456:2471	cancer treatment	2456:2471	The crude and partially purified IPSs would be potential materials for further study relating to cancer treatment.					
33773573	4	47	theme	polycephalum	704:715	arg1	microplasmodia					717:730	P. polycephalum microplasmodia	701:730	P. polycephalum microplasmodia	701:730	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	8	48	dep	showed	1254:1259	arg1	inhibited					1366:1374	inhibited	1366:1374	showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells	1254:1387	Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells.					
33773573	11	49	dep	have	1847:1850	arg1	reduced					1881:1887	reduced	1881:1887	reduced amount of that component	1881:1912	This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component.					
33773573	11	49	dep	have	1847:1850	arg1	either					1852:1857	either	1852:1857	either	1852:1857	This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component.					
33773573	7	50	theme	EPS	1146:1148	arg1	galactose					1154:1162	galactose	1154:1162	galactose (80.22%)	1154:1171	The major monomer of the EPS was galactose (80.22%), while that of the IPS was glucose (84.46%).					
33773573	7	50	theme	EPS	1146:1148	arg1	monomer					1131:1137	The major monomer	1121:1137	The major monomer of the EPS	1121:1148	The major monomer of the EPS was galactose (80.22%), while that of the IPS was glucose (84.46%).					
33773573	7	50	theme	EPS	1146:1148	arg1	EPS					1146:1148	the EPS	1142:1148	the EPS	1142:1148	The major monomer of the EPS was galactose (80.22%), while that of the IPS was glucose (84.46%).					
33773573	14	51	theme	further	2430:2436	arg1	study					2438:2442	further study	2430:2442	further study relating to cancer treatment	2430:2471	The crude and partially purified IPSs would be potential materials for further study relating to cancer treatment.					
33773573	13	52	contain	had	2161:2163	arg1	IPS					2123:2125	IPS	2123:2125	IPS	2123:2125	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	52	contain	had	2161:2163	arg2	carbohydrate					2205:2216	carbohydrate	2205:2216	carbohydrate	2205:2216	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	52	contain	had	2161:2163	arg2	weights					2302:2308	molecular weights	2292:2308	molecular weights	2292:2308	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	52	contain	had	2161:2163	arg2	protein					2219:2225	protein	2219:2225	protein	2219:2225	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	52	contain	had	2161:2163	arg1	CONCLUSIONS					2099:2109	CONCLUSIONS	2099:2109	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia	2099:2159	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	52	contain	had	2161:2163	arg2	composition					2276:2286	monosaccharide composition	2261:2286	monosaccharide composition	2261:2286	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	52	contain	had	2161:2163	arg1	EPS					2115:2117	EPS	2115:2117	EPS	2115:2117	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	52	contain	had	2161:2163	arg2	properties					2184:2193	different chemical properties	2165:2193	different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights	2165:2308	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	10	53	theme	MCF-7	1529:1533	arg1	cells					1535:1539	only 34.76 ± 6.58% MCF-7 cells	1510:1539	only 34.76 ± 6.58% MCF-7 cells	1510:1539	However, although only 34.76 ± 6.58% MCF-7 cells were viable when exposed to the crude IPS, but the partially purified IPS displayed non-toxicity to MCF-7 cells.					
33773573	1	54	contain	possess	209:215	arg2	bioactivities					228:240	remarkable bioactivities	217:240	remarkable bioactivities	217:240	BACKGROUND Microbial polysaccharides have been reported to possess remarkable bioactivities.					
33773573	1	54	contain	possess	209:215	arg1	polysaccharides					171:185	BACKGROUND Microbial polysaccharides	150:185	BACKGROUND Microbial polysaccharides	150:185	BACKGROUND Microbial polysaccharides have been reported to possess remarkable bioactivities.					
33773573	7	55	theme	major	1125:1129	arg1	galactose					1154:1162	galactose	1154:1162	galactose (80.22%)	1154:1171	The major monomer of the EPS was galactose (80.22%), while that of the IPS was glucose (84.46%).					
33773573	7	55	theme	major	1125:1129	arg1	monomer					1131:1137	The major monomer	1121:1137	The major monomer of the EPS	1121:1148	The major monomer of the EPS was galactose (80.22%), while that of the IPS was glucose (84.46%).					
33773573	7	55	theme	major	1125:1129	arg1	EPS					1146:1148	the EPS	1142:1148	the EPS	1142:1148	The major monomer of the EPS was galactose (80.22%), while that of the IPS was glucose (84.46%).					
33773573	2	56	theme	rapid	336:340	arg1	growth					342:347	rapid growth	336:347	rapid growth	336:347	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	9	57	theme	Hela	1409:1412	arg1	cells					1414:1418	Only 38.42 ± 2.84% Hela cells	1390:1418	Only 38.42 ± 2.84% Hela cells	1390:1418	Only 38.42 ± 2.84% Hela cells remained viable when treated with the partially purified IPS (1 mg/ml).					
33773573	12	58	theme	flow	1938:1941	arg1	cytometry					1943:1951	flow cytometry	1938:1951	flow cytometry	1938:1951	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	12	59	theme	purified	2046:2053	arg1	due					2081:2083	due	2081:2083	due	2081:2083	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	12	59	theme	purified	2046:2053	arg1	IPS					2055:2057	the partially purified IPS	2032:2057	the partially purified IPS toward Hela cells	2032:2075	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	2	60	theme	significant	367:377	arg1	amount					379:384	a significant amount	365:384	a significant amount of cell wall-less biomass	365:410	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	2	60	theme	significant	367:377	arg1	biomass					404:410	cell wall-less biomass	389:410	cell wall-less biomass	389:410	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	4	61	theme	cancer	788:793	arg1	lines					800:804	several cancer cell lines	780:804	several cancer cell lines	780:804	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	11	62	theme	IPS	1761:1763	arg1	sample					1765:1770	the crude IPS sample	1751:1770	the crude IPS sample (e.g. proteins, peptides or ion metals)	1751:1810	This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component.					
33773573	9	63	dep	cells	1414:1418	arg1	%					1407:1407	%	1407:1407	%	1407:1407	Only 38.42 ± 2.84% Hela cells remained viable when treated with the partially purified IPS (1 mg/ml).					
33773573	13	64	theme	total	2231:2235	arg1	contents					2251:2258	total sulfate group contents	2231:2258	total sulfate group contents	2231:2258	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	12	65	theme	Cell	1915:1918	arg1	analysis					1926:1933	Cell cycle analysis	1915:1933	Cell cycle analysis by flow cytometry	1915:1951	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	13	66	dep	CONCLUSIONS	2099:2109	arg1	IPS					2123:2125	IPS	2123:2125	IPS	2123:2125	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	66	dep	CONCLUSIONS	2099:2109	arg1	EPS					2115:2117	EPS	2115:2117	EPS	2115:2117	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	66	dep	CONCLUSIONS	2099:2109	arg1	CONCLUSIONS					2099:2109	CONCLUSIONS	2099:2109	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia	2099:2159	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	11	67	theme	ion	1800:1802	arg1	metals					1804:1809	ion metals	1800:1809	ion metals	1800:1809	This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component.					
33773573	13	68	theme	group	2245:2249	arg1	contents					2251:2258	total sulfate group contents	2231:2258	total sulfate group contents	2231:2258	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	10	69	theme	crude	1573:1577	arg1	IPS					1579:1581	the crude IPS	1569:1581	the crude IPS	1569:1581	However, although only 34.76 ± 6.58% MCF-7 cells were viable when exposed to the crude IPS, but the partially purified IPS displayed non-toxicity to MCF-7 cells.					
33773573	2	70	theme	mold	287:290	arg1	polycephalum					252:263	Physarum polycephalum	243:263	Physarum polycephalum	243:263	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	2	70	theme	mold	287:290	arg1	species					270:276	a species	268:276	a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass	268:410	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	1	71	theme	Microbial	161:169	arg1	polysaccharides					171:185	BACKGROUND Microbial polysaccharides	150:185	BACKGROUND Microbial polysaccharides	150:185	BACKGROUND Microbial polysaccharides have been reported to possess remarkable bioactivities.					
33773573	13	72	theme	molecular	2292:2300	arg1	weights					2302:2308	molecular weights	2292:2308	molecular weights	2292:2308	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	5	73	theme	crude	969:973	arg1	3.46 ± 0.36 g/l					980:994	3.46 ± 0.36 g/l	980:994	3.46 ± 0.36 g/l	980:994	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	5	73	theme	crude	969:973	arg1	IPS					975:977	the respective crude IPS	954:977	the respective crude IPS (3.46 ± 0.36 g/l)	954:995	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	12	74	theme	IPS	2011:2013	arg1	toxicity					1989:1996	the toxicity	1985:1996	the toxicity of the crude IPS toward MCF-7	1985:2026	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	0	75	theme	polysaccharides	93:107	arg1	activities					13:22	Cytotoxicity activities	0:22	Cytotoxicity activities	0:22	Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia.					
33773573	0	75	theme	polysaccharides	93:107	arg1	characteristics					37:51	chemical characteristics	28:51	chemical characteristics	28:51	Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia.					
33773573	11	76	theme	component	1904:1912	arg1	amount					1889:1894	amount	1889:1894	amount of that component	1889:1912	This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component.					
33773573	11	76	theme	component	1904:1912	arg1	component					1904:1912	that component	1899:1912	that component	1899:1912	This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component.					
33773573	13	77	theme	different	2165:2173	arg1	properties					2184:2193	different chemical properties	2165:2193	different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights	2165:2308	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	77	theme	different	2165:2173	arg1	composition					2276:2286	monosaccharide composition	2261:2286	monosaccharide composition	2261:2286	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	77	theme	different	2165:2173	arg1	protein					2219:2225	protein	2219:2225	protein	2219:2225	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	77	theme	different	2165:2173	arg1	weights					2302:2308	molecular weights	2292:2308	molecular weights	2292:2308	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	13	77	theme	different	2165:2173	arg1	carbohydrate					2205:2216	carbohydrate	2205:2216	carbohydrate	2205:2216	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	6	78	theme	molecular	1055:1063	arg1	14,762 kDa					1096:1105	14,762 kDa	1096:1105	14,762 kDa	1096:1105	The average molecular weight of the EPS and IPS were 14,762 kDa and 1788 kDa.					
33773573	6	78	theme	molecular	1055:1063	arg1	weight					1065:1070	The average molecular weight	1043:1070	The average molecular weight of the EPS and IPS	1043:1089	The average molecular weight of the EPS and IPS were 14,762 kDa and 1788 kDa.					
33773573	4	79	theme	primary	555:561	arg1	objectives					563:572	the primary objectives	551:572	the primary objectives of this research	551:589	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	12	80	theme	toxicity	1989:1996	arg1	due					2081:2083	due	2081:2083	due	2081:2083	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	12	80	theme	toxicity	1989:1996	arg1	IPS					2055:2057	the partially purified IPS	2032:2057	the partially purified IPS toward Hela cells	2032:2075	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	12	80	theme	toxicity	1989:1996	arg1	mechanism					1972:1980	the mechanism	1968:1980	the mechanism of the toxicity of the crude IPS toward MCF-7	1968:2026	Cell cycle analysis by flow cytometry suggested that the mechanism of the toxicity of the crude IPS toward MCF-7 and the partially purified IPS toward Hela cells was due to apoptosis.					
33773573	0	81	theme	microplasmodia	134:147	arg1	exopolysaccharides					56:73	exopolysaccharides	56:73	exopolysaccharides	56:73	Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia.					
33773573	0	81	theme	microplasmodia	134:147	arg1	polysaccharides					93:107	intracellular polysaccharides	79:107	intracellular polysaccharides	79:107	Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia.					
33773573	13	82	dep	EPS	2115:2117	arg1	The					2111:2113	The	2111:2113	The	2111:2113	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	8	83	theme	purified	1329:1336	arg1	sample					1338:1343	the purified sample	1325:1343	the purified sample	1325:1343	Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells.					
33773573	8	83	theme	purified	1329:1336	arg1	cells					1307:1311	Hela cells	1302:1311	Hela cells	1302:1311	Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells.					
33773573	3	84	theme	slime	505:509	arg1	molds					511:515	slime molds	505:515	slime molds	505:515	There has been a limited understanding of the polysaccharides produced by microplasmodia of slime molds, including P. polycephalum.					
33773573	11	85	dep	proteins	1778:1785	arg1	e.g.					1773:1776	e.g.	1773:1776	e.g.	1773:1776	This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component.					
33773573	8	86	dep	crude	1223:1227	arg1	samples					1246:1252	samples	1246:1252	samples	1246:1252	Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells.					
33773573	0	87	theme	chemical	28:35	arg1	characteristics					37:51	chemical characteristics	28:51	chemical characteristics	28:51	Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia.					
33773573	6	88	theme	IPS	1087:1089	arg1	14,762 kDa					1096:1105	14,762 kDa	1096:1105	14,762 kDa	1096:1105	The average molecular weight of the EPS and IPS were 14,762 kDa and 1788 kDa.					
33773573	6	88	theme	IPS	1087:1089	arg1	weight					1065:1070	The average molecular weight	1043:1070	The average molecular weight of the EPS and IPS	1043:1089	The average molecular weight of the EPS and IPS were 14,762 kDa and 1788 kDa.					
33773573	5	89	theme	EPS	839:841	arg1	yields					819:824	The yields	815:824	The yields of the crude EPS (4.43 ± 0.44 g/l)	815:859	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	6	90	theme	EPS	1079:1081	arg1	14,762 kDa					1096:1105	14,762 kDa	1096:1105	14,762 kDa	1096:1105	The average molecular weight of the EPS and IPS were 14,762 kDa and 1788 kDa.					
33773573	6	90	theme	EPS	1079:1081	arg1	weight					1065:1070	The average molecular weight	1043:1070	The average molecular weight of the EPS and IPS	1043:1089	The average molecular weight of the EPS and IPS were 14,762 kDa and 1788 kDa.					
33773573	4	91	theme	research	582:589	arg1	objectives					563:572	the primary objectives	551:572	the primary objectives of this research	551:589	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	5	92	dep	comparable	927:936	arg1	p > 0.05					939:946	p > 0.05	939:946	p > 0.05	939:946	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	13	93	theme	different	2324:2332	arg1	activities					2347:2356	different cytotoxicity activities	2324:2356	different cytotoxicity activities	2324:2356	CONCLUSIONS The EPS and IPS of P. polycephalum microplasmodia had different chemical properties including carbohydrate, protein and total sulfate group contents, monosaccharide composition and molecular weights, which led to different cytotoxicity activities.					
33773573	5	94	with	comparable	927:936	arg1	2.45 ± 0.36 g/l					1025:1039	2.45 ± 0.36 g/l	1025:1039	2.45 ± 0.36 g/l	1025:1039	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	5	94	with	comparable	927:936	arg1	IPS					1020:1022	partially purified IPS	1001:1022	partially purified IPS (2.45 ± 0.36 g/l)	1001:1040	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	5	94	with	comparable	927:936	arg1	3.46 ± 0.36 g/l					980:994	3.46 ± 0.36 g/l	980:994	3.46 ± 0.36 g/l	980:994	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	5	94	with	comparable	927:936	arg1	IPS					975:977	the respective crude IPS	954:977	the respective crude IPS (3.46 ± 0.36 g/l)	954:995	RESULTS The yields of the crude EPS (4.43 ± 0.44 g/l) and partially purified (deproteinated) EPS (2.95 ± 0.85 g/l) were comparable (p > 0.05) with the respective crude IPS (3.46 ± 0.36 g/l) and partially purified IPS (2.45 ± 0.36 g/l).					
33773573	8	95	theme	normal	1376:1381	arg1	cells					1383:1387	normal cells	1376:1387	normal cells	1376:1387	Both crude and purified IPS samples showed significantly higher cytotoxicity toward Hela cells, especially the purified sample and none of the IPSs inhibited normal cells.					
33773573	2	96	theme	cell	389:392	arg1	biomass					404:410	cell wall-less biomass	389:410	cell wall-less biomass	389:410	Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass.					
33773573	11	97	dep	either	1852:1857	arg1	removed					1870:1876	removed	1870:1876	removed	1870:1876	This suggested that the cytotoxicity toward MCF-7 would come from some component associated with the crude IPS sample (e.g. proteins, peptides or ion metals) and the purification process would have either completely removed or reduced amount of that component.					
33773573	4	98	theme	intracellular	662:674	arg1	IPS					693:695	IPS	693:695	IPS	693:695	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	4	98	theme	intracellular	662:674	arg1	polysaccharides					676:690	intracellular polysaccharides	662:690	intracellular polysaccharides (IPS)	662:696	Thus, the primary objectives of this research were first to chemically characterize the exopolysaccharides (EPS) and intracellular polysaccharides (IPS) of P. polycephalum microplasmodia and then to evaluate their cytotoxicity against several cancer cell lines.					
33773573	14	99	theme	crude	2363:2367	arg1	materials					2416:2424	potential materials	2406:2424	potential materials for further study relating to cancer treatment	2406:2471	The crude and partially purified IPSs would be potential materials for further study relating to cancer treatment.					
33773573	14	99	theme	crude	2363:2367	arg1	IPSs					2392:2395	The crude and partially purified IPSs	2359:2395	The crude and partially purified IPSs	2359:2395	The crude and partially purified IPSs would be potential materials for further study relating to cancer treatment.					
33773573	10	100	theme	MCF-7	1641:1645	arg1	cells					1647:1651	MCF-7 cells	1641:1651	MCF-7 cells	1641:1651	However, although only 34.76 ± 6.58% MCF-7 cells were viable when exposed to the crude IPS, but the partially purified IPS displayed non-toxicity to MCF-7 cells.					
34857783	0	0	theme	seedlings	75:83	arg1	composition					52:62	cell wall composition	42:62	cell wall composition of sorghum seedlings	42:83	Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings.					
34857783	6	1	dep	-β-D-glucan	1162:1172	arg1	1 → 4					1156:1160	1 → 4	1156:1160	1 → 4	1156:1160	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	0	2	theme	sorghum	67:73	arg1	seedlings					75:83	sorghum seedlings	67:83	sorghum seedlings	67:83	Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings.					
34857783	4	3	theme	cell	601:604	arg1	walls					606:610	The cell walls	597:610	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions	597:704	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	5	4	theme	supply	859:864	arg1	level					848:852	the level	844:852	the level of N supply	844:864	We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings.					
34857783	2	5	theme	wall	458:461	arg1	components					463:472	other cell wall components	447:472	other cell wall components	447:472	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	6	6	theme	mixed	1132:1136	arg1	1 → 3					1147:1151	1 → 3	1147:1151	1 → 3	1147:1151	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	6	theme	mixed	1132:1136	arg1	linkage					1138:1144	mixed linkage	1132:1144	mixed linkage (1 → 3)	1132:1152	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	7	theme	distribution	1082:1093	arg1	alteration					1061:1070	alteration	1061:1070	alteration	1061:1070	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	7	theme	distribution	1082:1093	arg1	distribution					1082:1093	tissue distribution	1075:1093	tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan	1075:1190	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	7	theme	distribution	1082:1093	arg1	amount					1050:1055	amount	1050:1055	amount	1050:1055	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	4	8	theme	deficient	683:691	arg1	conditions					695:704	sufficient or deficient N conditions	669:704	sufficient or deficient N conditions	669:704	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	2	9	theme	cell	453:456	arg1	components					463:472	other cell wall components	447:472	other cell wall components	447:472	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	4	10	theme	immunohistochemical	801:819	arg1	methods					821:827	immunohistochemical methods	801:827	immunohistochemical methods	801:827	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	5	11	theme	wall	897:900	arg1	structure					902:910	cell wall structure	892:910	cell wall structure	892:910	We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings.					
34857783	4	12	theme	cultured	630:637	arg1	seedlings					647:655	hydroponically cultured sorghum seedlings	615:655	hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions	615:704	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	2	13	theme	other	447:451	arg1	components					463:472	other cell wall components	447:472	other cell wall components	447:472	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	8	14	theme	cell	1348:1351	arg1	properties					1358:1367	the cell wall properties	1344:1367	the cell wall properties of sorghum seedlings	1344:1388	Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.					
34857783	4	15	dep	chemical	726:733	arg1	two-dimensional					736:750	two-dimensional	736:750	two-dimensional	736:750	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	2	16	theme	N	232:232	arg1	supply					235:240	nitrogen (N) supply	222:240	nitrogen (N) supply	222:240	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	8	17	theme	wall	1353:1356	arg1	properties					1358:1367	the cell wall properties	1344:1367	the cell wall properties of sorghum seedlings	1344:1388	Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.					
34857783	6	18	theme	syringyl/guaiacyl	991:1007	arg1	ratio					1021:1025	the syringyl/guaiacyl lignin unit ratio	987:1025	the syringyl/guaiacyl lignin unit ratio	987:1025	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	19	theme	tissue	1075:1080	arg1	distribution					1082:1093	tissue distribution	1075:1093	tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan	1075:1190	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	4	20	theme	sufficient	669:678	arg1	conditions					695:704	sufficient or deficient N conditions	669:704	sufficient or deficient N conditions	669:704	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	0	21	theme	Nitrogen	0:7	arg1	deficiency					9:18	Nitrogen deficiency	0:18	Nitrogen deficiency	0:18	Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings.					
34857783	4	22	theme	gene	780:783	arg1	expression					785:794	gene expression	780:794	gene expression	780:794	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	6	23	from	decrease	975:982	arg1	ratio					1021:1025	the syringyl/guaiacyl lignin unit ratio	987:1025	the syringyl/guaiacyl lignin unit ratio	987:1025	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	23	from	decrease	975:982	arg1	alteration					1061:1070	alteration	1061:1070	alteration	1061:1070	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	23	from	decrease	975:982	arg1	distribution					1082:1093	tissue distribution	1075:1093	tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan	1075:1190	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	23	from	decrease	975:982	arg1	amount					1050:1055	amount	1050:1055	amount	1050:1055	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	1	24	theme	Sorghum	86:92	arg1	Moench					116:121	L.) Moench	112:121	L.) Moench	112:121	Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production.					
34857783	1	24	theme	Sorghum	86:92	arg1	bicolor					103:109	Sorghum [Sorghum bicolor	86:109	Sorghum [Sorghum bicolor (L.) Moench]	86:122	Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production.					
34857783	7	25	from	changes	1263:1269	arg1	expression					1279:1288	gene expression	1274:1288	gene expression	1274:1288	At least some of these cell wall alterations could be associated with changes in gene expression.					
34857783	7	26	theme	cell	1216:1219	arg1	alterations					1226:1236	these cell wall alterations	1210:1236	these cell wall alterations	1210:1236	At least some of these cell wall alterations could be associated with changes in gene expression.					
34857783	5	27	theme	sorghum	931:937	arg1	seedlings					939:947	sorghum seedlings	931:947	sorghum seedlings	931:947	We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings.					
34857783	5	28	theme	N	857:857	arg1	supply					859:864	N supply	857:864	N supply	857:864	We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings.					
34857783	2	29	theme	sorghum	264:270	arg1	growth					272:277	sorghum growth	264:277	sorghum growth	264:277	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	8	30	theme	Nitrogen	1291:1298	arg1	status					1300:1305	Nitrogen status	1291:1305	Nitrogen status	1291:1305	Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.					
34857783	2	31	theme	biomass	369:375	arg1	quality					377:383	the biomass quality	365:383	the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components	365:472	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	4	32	theme	seedlings	647:655	arg1	walls					606:610	The cell walls	597:610	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions	597:704	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	2	33	theme	sorghum	388:394	arg1	quality					377:383	the biomass quality	365:383	the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components	365:472	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	6	34	theme	hemicelluloses	1106:1119	arg1	distribution					1082:1093	tissue distribution	1075:1093	tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan	1075:1190	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	35	theme	unit	1016:1019	arg1	ratio					1021:1025	the syringyl/guaiacyl lignin unit ratio	987:1025	the syringyl/guaiacyl lignin unit ratio	987:1025	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	1	36	theme	biomass	170:176	arg1	production					185:194	biomass energy production	170:194	biomass energy production	170:194	Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production.					
34857783	5	37	theme	cell	892:895	arg1	structure					902:910	cell wall structure	892:910	cell wall structure	892:910	We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings.					
34857783	8	38	theme	factors	1326:1332	arg1	one					1315:1317	one	1315:1317	one	1315:1317	Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.					
34857783	8	38	theme	factors	1326:1332	arg1	factors					1326:1332	the factors	1322:1332	the factors affecting the cell wall properties of sorghum seedlings	1322:1388	Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.					
34857783	7	39	theme	gene	1274:1277	arg1	expression					1279:1288	gene expression	1274:1288	gene expression	1274:1288	At least some of these cell wall alterations could be associated with changes in gene expression.					
34857783	3	40	from	effects	521:527	arg1	composition					562:572	composition	562:572	composition	562:572	Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls.					
34857783	3	40	from	effects	521:527	arg1	structure					548:556	structure	548:556	structure	548:556	Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls.					
34857783	1	41	theme	energy	178:183	arg1	production					185:194	biomass energy production	170:194	biomass energy production	170:194	Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production.					
34857783	6	42	theme	lignin	1009:1014	arg1	ratio					1021:1025	the syringyl/guaiacyl lignin unit ratio	987:1025	the syringyl/guaiacyl lignin unit ratio	987:1025	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	7	43	theme	wall	1221:1224	arg1	alterations					1226:1236	these cell wall alterations	1210:1236	these cell wall alterations	1210:1236	At least some of these cell wall alterations could be associated with changes in gene expression.					
34857783	2	44	theme	N	360:360	arg1	effect					350:355	the effect	346:355	the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components	346:472	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	0	45	theme	wall	47:50	arg1	composition					52:62	cell wall composition	42:62	cell wall composition of sorghum seedlings	42:83	Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings.					
34857783	4	46	theme	magnetic	760:767	arg1	resonance					769:777	chemical, two-dimensional nuclear magnetic resonance	726:777	chemical, two-dimensional nuclear magnetic resonance	726:777	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	6	47	from	increase	1034:1041	arg1	ratio					1021:1025	the syringyl/guaiacyl lignin unit ratio	987:1025	the syringyl/guaiacyl lignin unit ratio	987:1025	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	47	from	increase	1034:1041	arg1	alteration					1061:1070	alteration	1061:1070	alteration	1061:1070	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	47	from	increase	1034:1041	arg1	distribution					1082:1093	tissue distribution	1075:1093	tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan	1075:1190	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	47	from	increase	1034:1041	arg1	amount					1050:1055	amount	1050:1055	amount	1050:1055	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	5	48	dep	structure	902:910	arg1	the					888:890	the	888:890	the	888:890	We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings.					
34857783	0	49	theme	cell	42:45	arg1	composition					52:62	cell wall composition	42:62	cell wall composition of sorghum seedlings	42:83	Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings.					
34857783	6	50	theme	several	1098:1104	arg1	linkage					1138:1144	mixed linkage	1132:1144	mixed linkage (1 → 3)	1132:1152	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	50	theme	several	1098:1104	arg1	-β-D-glucan					1162:1172	(1 → 4)-β-D-glucan	1155:1172	(1 → 4)-β-D-glucan	1155:1172	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	50	theme	several	1098:1104	arg1	arabinoxylan					1179:1190	arabinoxylan	1179:1190	arabinoxylan	1179:1190	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	50	theme	several	1098:1104	arg1	hemicelluloses					1106:1119	several hemicelluloses	1098:1119	several hemicelluloses	1098:1119	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	6	51	dep	amount	1050:1055	arg1	the					1046:1048	the	1046:1048	the	1046:1048	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	3	52	theme	cell	585:588	arg1	walls					590:594	sorghum cell walls	577:594	sorghum cell walls	577:594	Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls.					
34857783	4	53	theme	nuclear	752:758	arg1	resonance					769:777	chemical, two-dimensional nuclear magnetic resonance	726:777	chemical, two-dimensional nuclear magnetic resonance	726:777	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	1	54	theme	L.	112:113	arg1	Moench					116:121	L.) Moench	112:121	L.) Moench	112:121	Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production.					
34857783	1	54	theme	L.	112:113	arg1	bicolor					103:109	Sorghum [Sorghum bicolor	86:109	Sorghum [Sorghum bicolor (L.) Moench]	86:122	Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production.					
34857783	5	55	theme	seedlings	939:947	arg1	composition					916:926	composition	916:926	composition	916:926	We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings.					
34857783	5	55	theme	seedlings	939:947	arg1	structure					902:910	cell wall structure	892:910	cell wall structure	892:910	We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings.					
34857783	3	56	theme	walls	590:594	arg1	composition					562:572	composition	562:572	composition	562:572	Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls.					
34857783	3	56	theme	walls	590:594	arg1	structure					548:556	structure	548:556	structure	548:556	Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls.					
34857783	3	57	theme	supply	534:539	arg1	effects					521:527	the effects	517:527	the effects of N supply on the structure and composition of sorghum cell walls	517:594	Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls.					
34857783	2	58	from	effect	350:355	arg1	quality					377:383	the biomass quality	365:383	the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components	365:472	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	4	59	theme	sorghum	639:645	arg1	seedlings					647:655	hydroponically cultured sorghum seedlings	615:655	hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions	615:704	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	1	60	theme	[Sorghum	94:101	arg1	Moench					116:121	L.) Moench	112:121	L.) Moench	112:121	Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production.					
34857783	1	60	theme	[Sorghum	94:101	arg1	bicolor					103:109	Sorghum [Sorghum bicolor	86:109	Sorghum [Sorghum bicolor (L.) Moench]	86:122	Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production.					
34857783	6	61	theme	N	964:964	arg1	Limitation					950:959	Limitation	950:959	Limitation of N	950:964	Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-D-glucan, and arabinoxylan.					
34857783	4	62	theme	N	693:693	arg1	conditions					695:704	sufficient or deficient N conditions	669:704	sufficient or deficient N conditions	669:704	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	8	63	theme	sorghum	1372:1378	arg1	seedlings					1380:1388	sorghum seedlings	1372:1388	sorghum seedlings	1372:1388	Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.					
34857783	2	64	theme	nitrogen	222:229	arg1	supply					235:240	nitrogen (N) supply	222:240	nitrogen (N) supply	222:240	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	8	65	theme	seedlings	1380:1388	arg1	properties					1358:1367	the cell wall properties	1344:1367	the cell wall properties of sorghum seedlings	1344:1388	Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.					
34857783	2	66	theme	biomass	283:289	arg1	accumulation					291:302	biomass accumulation	283:302	biomass accumulation	283:302	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	3	67	dep	structure	548:556	arg1	the					544:546	the	544:546	the	544:546	Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls.					
34857783	2	68	theme	lignin	436:441	arg1	components					463:472	other cell wall components	447:472	other cell wall components	447:472	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	2	68	theme	lignin	436:441	arg1	structures					422:431	structures	422:431	structures	422:431	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	2	68	theme	lignin	436:441	arg1	contents					409:416	the contents	405:416	the contents	405:416	While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components.					
34857783	3	69	theme	sorghum	577:583	arg1	walls					590:594	sorghum cell walls	577:594	sorghum cell walls	577:594	Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls.					
34857783	4	70	theme	chemical	726:733	arg1	resonance					769:777	chemical, two-dimensional nuclear magnetic resonance	726:777	chemical, two-dimensional nuclear magnetic resonance	726:777	The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods.					
34857783	3	71	theme	N	532:532	arg1	supply					534:539	N supply	532:539	N supply	532:539	Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls.					
34919279	0	0	theme	Patinopecten	77:88	arg1	gum					145:147	scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	68:147	scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	68:147	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	4	1	theme	bonds	672:676	arg1	generation					649:658	the generation	645:658	the generation of hydrogen bonds between SMGHs and KC/XG	645:700	FTIR spectrum showed the generation of hydrogen bonds between SMGHs and KC/XG, and the carboxylic acid group of XG interacts with SMGHs.					
34919279	1	2	theme	study	172:176	arg1	objective					154:162	The objective	150:162	The objective of this study	150:176	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	3	theme	yessoensis	262:271	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	3	theme	yessoensis	262:271	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	10	4	theme	Further	1600:1606	arg1	study					1608:1612	Further study	1600:1612	Further study	1600:1612	Further study is undergoing to apply SMGHs/KC/XG to embed bioactive compounds, such as curcumin and β-carotene.					
34919279	4	5	theme	hydrogen	663:670	arg1	bonds					672:676	hydrogen bonds	663:676	hydrogen bonds	663:676	FTIR spectrum showed the generation of hydrogen bonds between SMGHs and KC/XG, and the carboxylic acid group of XG interacts with SMGHs.					
34919279	8	6	contain	have	1384:1387	arg2	potential					1389:1397	potential	1389:1397	potential	1389:1397	Because of its gelation properties, scallop male gonads have potential to be used as functional hydrogels for food.					
34919279	8	6	contain	have	1384:1387	arg1	gonads					1377:1382	scallop male gonads	1364:1382	scallop male gonads	1364:1382	Because of its gelation properties, scallop male gonads have potential to be used as functional hydrogels for food.					
34919279	9	7	theme	biological	1581:1590	arg1	fields					1592:1597	food and biological fields	1572:1597	food and biological fields	1572:1597	The SMGHs/KC/XG ternary composite gel showed excellent gel properties, which would be potentially applied in delivery system in food and biological fields.					
34919279	6	8	theme	KC	1045:1046	arg1	addition					1033:1040	addition	1033:1040	addition of KC and XG	1033:1053	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	9	9	theme	ternary	1460:1466	arg1	gel					1478:1480	The SMGHs/KC/XG ternary composite gel	1444:1480	The SMGHs/KC/XG ternary composite gel	1444:1480	The SMGHs/KC/XG ternary composite gel showed excellent gel properties, which would be potentially applied in delivery system in food and biological fields.					
34919279	7	10	theme	adductor	1307:1314	arg1	processing					1316:1325	adductor processing	1307:1325	adductor processing	1307:1325	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	9	11	theme	gel	1499:1501	arg1	properties					1503:1512	excellent gel properties	1489:1512	excellent gel properties	1489:1512	The SMGHs/KC/XG ternary composite gel showed excellent gel properties, which would be potentially applied in delivery system in food and biological fields.					
34919279	8	12	theme	male	1372:1375	arg1	gonads					1377:1382	scallop male gonads	1364:1382	scallop male gonads	1364:1382	Because of its gelation properties, scallop male gonads have potential to be used as functional hydrogels for food.					
34919279	1	13	theme	protein	274:280	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	13	theme	protein	274:280	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	0	14	theme	yessoensis	90:99	arg1	gum					145:147	scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	68:147	scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	68:147	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	4	15	theme	acid	722:725	arg1	group					727:731	the carboxylic acid group	707:731	the carboxylic acid group of XG	707:737	FTIR spectrum showed the generation of hydrogen bonds between SMGHs and KC/XG, and the carboxylic acid group of XG interacts with SMGHs.					
34919279	4	15	theme	acid	722:725	arg1	XG					736:737	XG	736:737	XG	736:737	FTIR spectrum showed the generation of hydrogen bonds between SMGHs and KC/XG, and the carboxylic acid group of XG interacts with SMGHs.					
34919279	3	16	theme	water	529:533	arg1	capacity					543:550	water holding capacity	529:550	water holding capacity	529:550	The results showed that the G' value, melting temperature, and water holding capacity of SMGHs/KC/XG were higher than those of SMGHs, SMGHs/KC, and SMGHs/XG.					
34919279	10	17	theme	embed	1652:1656	arg1	compounds					1668:1676	embed bioactive compounds	1652:1676	embed bioactive compounds	1652:1676	Further study is undergoing to apply SMGHs/KC/XG to embed bioactive compounds, such as curcumin and β-carotene.					
34919279	10	17	theme	embed	1652:1656	arg1	β-carotene					1700:1709	β-carotene	1700:1709	β-carotene	1700:1709	Further study is undergoing to apply SMGHs/KC/XG to embed bioactive compounds, such as curcumin and β-carotene.					
34919279	10	17	theme	embed	1652:1656	arg1	curcumin					1687:1694	curcumin	1687:1694	curcumin	1687:1694	Further study is undergoing to apply SMGHs/KC/XG to embed bioactive compounds, such as curcumin and β-carotene.					
34919279	1	18	theme	hydrolysates	282:293	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	18	theme	hydrolysates	282:293	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	0	19	theme	hydrolysates/κ-carrageenan/xanthan	110:143	arg1	gum					145:147	scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	68:147	scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	68:147	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	7	20	theme	yessoensis	1221:1230	arg1	byproducts					1289:1298	byproducts	1289:1298	byproducts during adductor processing	1289:1325	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	7	20	theme	yessoensis	1221:1230	arg1	gonads					1238:1243	Scallop (Patinopecten yessoensis) male gonads	1199:1243	Scallop (Patinopecten yessoensis) male gonads	1199:1243	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	6	21	theme	SMGHs	997:1001	arg1	properties					983:992	the gel and microstructural properties	955:992	properties	983:992	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	4	22	theme	XG	736:737	arg1	group					727:731	the carboxylic acid group	707:731	the carboxylic acid group of XG	707:737	FTIR spectrum showed the generation of hydrogen bonds between SMGHs and KC/XG, and the carboxylic acid group of XG interacts with SMGHs.					
34919279	4	22	theme	XG	736:737	arg1	XG					736:737	XG	736:737	XG	736:737	FTIR spectrum showed the generation of hydrogen bonds between SMGHs and KC/XG, and the carboxylic acid group of XG interacts with SMGHs.					
34919279	2	23	theme	molecular	391:399	arg1	structure					401:409	molecular structure	391:409	molecular structure	391:409	The rheological properties, moisture-distribution, molecular structure, and microstructure of SMGNs/KC/XG gels were analyzed.					
34919279	0	24	theme	protein	102:108	arg1	gum					145:147	scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	68:147	scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	68:147	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	9	25	theme	SMGHs/KC/XG	1448:1458	arg1	gel					1478:1480	The SMGHs/KC/XG ternary composite gel	1444:1480	The SMGHs/KC/XG ternary composite gel	1444:1480	The SMGHs/KC/XG ternary composite gel showed excellent gel properties, which would be potentially applied in delivery system in food and biological fields.					
34919279	9	26	from	system	1562:1567	arg1	fields					1592:1597	food and biological fields	1572:1597	food and biological fields	1572:1597	The SMGHs/KC/XG ternary composite gel showed excellent gel properties, which would be potentially applied in delivery system in food and biological fields.					
34919279	4	27	theme	FTIR	624:627	arg1	spectrum					629:636	FTIR spectrum	624:636	FTIR spectrum	624:636	FTIR spectrum showed the generation of hydrogen bonds between SMGHs and KC/XG, and the carboxylic acid group of XG interacts with SMGHs.					
34919279	9	28	theme	composite	1468:1476	arg1	gel					1478:1480	The SMGHs/KC/XG ternary composite gel	1444:1480	The SMGHs/KC/XG ternary composite gel	1444:1480	The SMGHs/KC/XG ternary composite gel showed excellent gel properties, which would be potentially applied in delivery system in food and biological fields.					
34919279	7	29	theme	Scallop	1199:1205	arg1	byproducts					1289:1298	byproducts	1289:1298	byproducts during adductor processing	1289:1325	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	7	29	theme	Scallop	1199:1205	arg1	gonads					1238:1243	Scallop (Patinopecten yessoensis) male gonads	1199:1243	Scallop (Patinopecten yessoensis) male gonads	1199:1243	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	7	30	theme	Patinopecten	1208:1219	arg1	byproducts					1289:1298	byproducts	1289:1298	byproducts during adductor processing	1289:1325	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	7	30	theme	Patinopecten	1208:1219	arg1	gonads					1238:1243	Scallop (Patinopecten yessoensis) male gonads	1199:1243	Scallop (Patinopecten yessoensis) male gonads	1199:1243	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	5	31	theme	tighter	828:834	arg1	microstructure					867:880	a tighter, smoother, and more aggregated microstructure	826:880	a tighter, smoother, and more aggregated microstructure	826:880	Moreover, the cryo-SEM results showed that SMGHs/KC/XG exhibited a tighter, smoother, and more aggregated microstructure than those of SMGHs, SMGHs/KC, and SMGHs/XG.					
34919279	2	32	theme	gels	446:449	arg1	microstructure					416:429	microstructure	416:429	microstructure	416:429	The rheological properties, moisture-distribution, molecular structure, and microstructure of SMGNs/KC/XG gels were analyzed.					
34919279	2	32	theme	gels	446:449	arg1	moisture-distribution					368:388	moisture-distribution	368:388	moisture-distribution	368:388	The rheological properties, moisture-distribution, molecular structure, and microstructure of SMGNs/KC/XG gels were analyzed.					
34919279	2	32	theme	gels	446:449	arg1	structure					401:409	molecular structure	391:409	molecular structure	391:409	The rheological properties, moisture-distribution, molecular structure, and microstructure of SMGNs/KC/XG gels were analyzed.					
34919279	2	32	theme	gels	446:449	arg1	properties					356:365	The rheological properties	340:365	The rheological properties	340:365	The rheological properties, moisture-distribution, molecular structure, and microstructure of SMGNs/KC/XG gels were analyzed.					
34919279	3	33	theme	SMGHs/KC/XG	555:565	arg1	value					497:501	the G' value	490:501	the G' value	490:501	The results showed that the G' value, melting temperature, and water holding capacity of SMGHs/KC/XG were higher than those of SMGHs, SMGHs/KC, and SMGHs/XG.					
34919279	3	33	theme	SMGHs/KC/XG	555:565	arg1	temperature					512:522	melting temperature	504:522	melting temperature	504:522	The results showed that the G' value, melting temperature, and water holding capacity of SMGHs/KC/XG were higher than those of SMGHs, SMGHs/KC, and SMGHs/XG.					
34919279	3	33	theme	SMGHs/KC/XG	555:565	arg1	capacity					543:550	water holding capacity	529:550	water holding capacity	529:550	The results showed that the G' value, melting temperature, and water holding capacity of SMGHs/KC/XG were higher than those of SMGHs, SMGHs/KC, and SMGHs/XG.					
34919279	9	34	theme	excellent	1489:1497	arg1	properties					1503:1512	excellent gel properties	1489:1512	excellent gel properties	1489:1512	The SMGHs/KC/XG ternary composite gel showed excellent gel properties, which would be potentially applied in delivery system in food and biological fields.					
34919279	2	35	theme	SMGNs/KC/XG	434:444	arg1	gels					446:449	SMGNs/KC/XG gels	434:449	SMGNs/KC/XG gels	434:449	The rheological properties, moisture-distribution, molecular structure, and microstructure of SMGNs/KC/XG gels were analyzed.					
34919279	1	36	theme	SMGHs	296:300	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	36	theme	SMGHs	296:300	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	9	37	theme	delivery	1553:1560	arg1	system					1562:1567	delivery system	1553:1567	delivery system in food and biological fields	1553:1597	The SMGHs/KC/XG ternary composite gel showed excellent gel properties, which would be potentially applied in delivery system in food and biological fields.					
34919279	3	38	theme	holding	535:541	arg1	capacity					543:550	water holding capacity	529:550	water holding capacity	529:550	The results showed that the G' value, melting temperature, and water holding capacity of SMGHs/KC/XG were higher than those of SMGHs, SMGHs/KC, and SMGHs/XG.					
34919279	0	39	theme	microstructural	13:27	arg1	properties					29:38	microstructural properties	13:38	microstructural properties	13:38	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	0	40	theme	gum	145:147	arg1	gel					61:63	ternary composite gel	43:63	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	43:147	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	10	41	theme	bioactive	1658:1666	arg1	compounds					1668:1676	embed bioactive compounds	1652:1676	embed bioactive compounds	1652:1676	Further study is undergoing to apply SMGHs/KC/XG to embed bioactive compounds, such as curcumin and β-carotene.					
34919279	10	41	theme	bioactive	1658:1666	arg1	β-carotene					1700:1709	β-carotene	1700:1709	β-carotene	1700:1709	Further study is undergoing to apply SMGHs/KC/XG to embed bioactive compounds, such as curcumin and β-carotene.					
34919279	10	41	theme	bioactive	1658:1666	arg1	curcumin					1687:1694	curcumin	1687:1694	curcumin	1687:1694	Further study is undergoing to apply SMGHs/KC/XG to embed bioactive compounds, such as curcumin and β-carotene.					
34919279	1	42	theme	ternary	215:221	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	42	theme	ternary	215:221	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	0	43	theme	ternary	43:49	arg1	gel					61:63	ternary composite gel	43:63	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	43:147	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	1	44	theme	/κ-carrageenan	302:315	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	44	theme	/κ-carrageenan	302:315	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	7	45	theme	male	1233:1236	arg1	byproducts					1289:1298	byproducts	1289:1298	byproducts during adductor processing	1289:1325	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	7	45	theme	male	1233:1236	arg1	gonads					1238:1243	Scallop (Patinopecten yessoensis) male gonads	1199:1243	Scallop (Patinopecten yessoensis) male gonads	1199:1243	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	6	46	theme	food	1125:1128	arg1	applications					1162:1173	food, pharmaceutical, and biomedical applications	1125:1173	food, pharmaceutical, and biomedical applications	1125:1173	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	1	47	theme	composite	223:231	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	47	theme	composite	223:231	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	6	48	theme	microstructural	967:981	arg1	properties					983:992	the gel and microstructural properties	955:992	properties	983:992	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	1	49	theme	gel	233:235	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	49	theme	gel	233:235	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	0	50	theme	gel	61:63	arg1	Gelation					0:7	Gelation	0:7	Gelation	0:7	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	0	50	theme	gel	61:63	arg1	properties					29:38	microstructural properties	13:38	microstructural properties	13:38	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	1	51	theme	KC	318:319	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	51	theme	KC	318:319	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	6	52	contain	has	1072:1074	arg2	potential					1076:1084	potential	1076:1084	potential	1076:1084	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	6	52	contain	has	1072:1074	arg1	SMGHs/KC/XG					1060:1070	SMGHs/KC/XG	1060:1070	SMGHs/KC/XG	1060:1070	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	6	53	theme	biomedical	1151:1160	arg1	applications					1162:1173	food, pharmaceutical, and biomedical applications	1125:1173	food, pharmaceutical, and biomedical applications	1125:1173	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	6	54	theme	XG	1052:1053	arg1	addition					1033:1040	addition	1033:1040	addition of KC and XG	1033:1053	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	5	55	theme	aggregated	856:865	arg1	microstructure					867:880	a tighter, smoother, and more aggregated microstructure	826:880	a tighter, smoother, and more aggregated microstructure	826:880	Moreover, the cryo-SEM results showed that SMGHs/KC/XG exhibited a tighter, smoother, and more aggregated microstructure than those of SMGHs, SMGHs/KC, and SMGHs/XG.					
34919279	0	56	theme	composite	51:59	arg1	gel					61:63	ternary composite gel	43:63	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	43:147	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	8	57	theme	gelation	1343:1350	arg1	properties					1352:1361	its gelation properties	1339:1361	its gelation properties	1339:1361	Because of its gelation properties, scallop male gonads have potential to be used as functional hydrogels for food.					
34919279	1	58	theme	/xanthan	321:328	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	58	theme	/xanthan	321:328	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	8	59	theme	scallop	1364:1370	arg1	gonads					1377:1382	scallop male gonads	1364:1382	scallop male gonads	1364:1382	Because of its gelation properties, scallop male gonads have potential to be used as functional hydrogels for food.					
34919279	7	60	from	rich	1249:1252	arg1	protein					1257:1263	protein	1257:1263	protein	1257:1263	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	4	61	theme	carboxylic	711:720	arg1	group					727:731	the carboxylic acid group	707:731	the carboxylic acid group of XG	707:737	FTIR spectrum showed the generation of hydrogen bonds between SMGHs and KC/XG, and the carboxylic acid group of XG interacts with SMGHs.					
34919279	4	61	theme	carboxylic	711:720	arg1	XG					736:737	XG	736:737	XG	736:737	FTIR spectrum showed the generation of hydrogen bonds between SMGHs and KC/XG, and the carboxylic acid group of XG interacts with SMGHs.					
34919279	1	62	theme	of	237:238	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	62	theme	of	237:238	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	6	63	theme	pharmaceutical	1131:1144	arg1	applications					1162:1173	food, pharmaceutical, and biomedical applications	1125:1173	food, pharmaceutical, and biomedical applications	1125:1173	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	3	64	theme	melting	504:510	arg1	temperature					512:522	melting temperature	504:522	melting temperature	504:522	The results showed that the G' value, melting temperature, and water holding capacity of SMGHs/KC/XG were higher than those of SMGHs, SMGHs/KC, and SMGHs/XG.					
34919279	1	65	theme	scallop	240:246	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	65	theme	scallop	240:246	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	0	66	theme	scallop	68:74	arg1	gum					145:147	scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	68:147	scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum	68:147	Gelation and microstructural properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates/κ-carrageenan/xanthan gum.					
34919279	8	67	theme	functional	1413:1422	arg1	hydrogels					1424:1432	functional hydrogels	1413:1432	functional hydrogels for food	1413:1441	Because of its gelation properties, scallop male gonads have potential to be used as functional hydrogels for food.					
34919279	9	68	theme	food	1572:1575	arg1	fields					1592:1597	food and biological fields	1572:1597	food and biological fields	1572:1597	The SMGHs/KC/XG ternary composite gel showed excellent gel properties, which would be potentially applied in delivery system in food and biological fields.					
34919279	7	69	from	protein	1257:1263	arg1	rich					1249:1252	rich	1249:1252	rich	1249:1252	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	5	70	theme	smoother	837:844	arg1	microstructure					867:880	a tighter, smoother, and more aggregated microstructure	826:880	a tighter, smoother, and more aggregated microstructure	826:880	Moreover, the cryo-SEM results showed that SMGHs/KC/XG exhibited a tighter, smoother, and more aggregated microstructure than those of SMGHs, SMGHs/KC, and SMGHs/XG.					
34919279	6	71	theme	functional	1100:1109	arg1	hydrogels					1111:1119	functional hydrogels	1100:1119	functional hydrogels for food, pharmaceutical, and biomedical applications	1100:1173	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	6	72	theme	gel	959:961	arg1	properties					983:992	the gel and microstructural properties	955:992	properties	983:992	These results indicate that the gel and microstructural properties of SMGHs are significantly improved by addition of KC and XG, and SMGHs/KC/XG has potential to be used as functional hydrogels for food, pharmaceutical, and biomedical applications.					
34919279	1	73	theme	gum	330:332	arg1	properties					201:210	the properties	197:210	the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	197:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	5	74	theme	cryo-SEM	775:782	arg1	results					784:790	the cryo-SEM results	771:790	the cryo-SEM results	771:790	Moreover, the cryo-SEM results showed that SMGHs/KC/XG exhibited a tighter, smoother, and more aggregated microstructure than those of SMGHs, SMGHs/KC, and SMGHs/XG.					
34919279	7	75	dep	APPLICATION	1186:1196	arg1	regarded					1277:1284	regarded	1277:1284	usually regarded as byproducts during adductor processing	1269:1325	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	7	75	dep	APPLICATION	1186:1196	arg1	rich					1249:1252	rich	1249:1252	rich	1249:1252	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	2	76	theme	rheological	344:354	arg1	properties					356:365	The rheological properties	340:365	The rheological properties	340:365	The rheological properties, moisture-distribution, molecular structure, and microstructure of SMGNs/KC/XG gels were analyzed.					
34919279	7	77	theme	PRACTICAL	1176:1184	arg1	APPLICATION					1186:1196	PRACTICAL APPLICATION	1176:1196	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.	1176:1326	PRACTICAL APPLICATION: Scallop (Patinopecten yessoensis) male gonads are rich in protein and usually regarded as byproducts during adductor processing.					
34919279	1	78	theme	Patinopecten	249:260	arg1	XG					335:336	XG	335:336	XG	335:336	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
34919279	1	78	theme	Patinopecten	249:260	arg1	gum					330:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum	215:332	ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG)	215:337	The objective of this study was to investigate the properties of ternary composite gel of scallop (Patinopecten yessoensis) protein hydrolysates (SMGHs)/κ-carrageenan (KC)/xanthan gum (XG).					
33275405	4	0	contain	have	772:775	arg1	hydrogels					707:715	tricomponent composite hydrogels	684:715	tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs	684:757	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	4	0	contain	have	772:775	arg2	performance					798:808	promising mechanical performance	777:808	promising mechanical performance	777:808	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	4	1	contain	containing	717:726	arg1	hydrogels					707:715	tricomponent composite hydrogels	684:715	tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs	684:757	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	4	1	contain	containing	717:726	arg2	ratio					739:743	a specific ratio	728:743	a specific ratio of CNCs/ChNFs	728:757	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	5	2	from	absorption	889:898	arg1	results					870:876	results	870:876	results from water absorption, rheological, and light scattering studies	870:941	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	3	3	theme	composite	511:519	arg1	hydrogels					521:529	composite hydrogels	511:529	composite hydrogels containing CNCs, ChNFs, or their mixtures	511:571	In this work, composite hydrogels containing CNCs, ChNFs, or their mixtures are produced using cyclic freeze-thaw (FT) treatments.					
33275405	0	4	theme	Nanocrystals	86:97	arg1	Mixtures					64:71	Nanofiber Mixtures	54:71	Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers	54:119	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.					
33275405	2	5	theme	composite	368:376	arg1	hydrogels					378:386	tricomponent composite hydrogels	355:386	tricomponent composite hydrogels	355:386	When used in tricomponent composite hydrogels, the opposite surface charges on CNCs and ChNFs lead to the construction of beneficial nanofiber structures.					
33275405	0	6	theme	Nanofibers	110:119	arg1	Mixtures					64:71	Nanofiber Mixtures	54:71	Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers	54:119	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.					
33275405	3	7	contain	containing	531:540	arg2	CNCs					542:545	CNCs	542:545	CNCs	542:545	In this work, composite hydrogels containing CNCs, ChNFs, or their mixtures are produced using cyclic freeze-thaw (FT) treatments.					
33275405	3	7	contain	containing	531:540	arg1	hydrogels					521:529	composite hydrogels	511:529	composite hydrogels containing CNCs, ChNFs, or their mixtures	511:571	In this work, composite hydrogels containing CNCs, ChNFs, or their mixtures are produced using cyclic freeze-thaw (FT) treatments.					
33275405	3	7	contain	containing	531:540	arg2	mixtures					564:571	their mixtures	558:571	their mixtures	558:571	In this work, composite hydrogels containing CNCs, ChNFs, or their mixtures are produced using cyclic freeze-thaw (FT) treatments.					
33275405	3	7	contain	containing	531:540	arg2	ChNFs					548:552	ChNFs	548:552	ChNFs	548:552	In this work, composite hydrogels containing CNCs, ChNFs, or their mixtures are produced using cyclic freeze-thaw (FT) treatments.					
33275405	4	8	theme	promising	777:785	arg1	performance					798:808	promising mechanical performance	777:808	promising mechanical performance	777:808	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	5	9	theme	property	989:996	arg1	improvement					998:1008	property improvement	989:1008	property improvement	989:1008	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	0	10	theme	Chitin	103:108	arg1	Nanofibers					110:119	Chitin Nanofibers	103:119	Chitin Nanofibers	103:119	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.					
33275405	5	11	theme	load	1135:1138	arg1	transfer					1140:1147	load transfer	1135:1147	load transfer	1135:1147	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	5	12	theme	light	918:922	arg1	studies					935:941	light scattering studies	918:941	light scattering studies	918:941	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	5	13	theme	water	883:887	arg1	absorption					889:898	water absorption	883:898	water absorption	883:898	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	4	14	theme	specific	730:737	arg1	ratio					739:743	a specific ratio	728:743	a specific ratio of CNCs/ChNFs	728:757	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	6	15	theme	nanofiber	1225:1233	arg1	structures					1235:1244	electrostatically driven nanofiber structures	1200:1244	electrostatically driven nanofiber structures in nanocomposites	1200:1262	Overall, these results provide insight into using electrostatically driven nanofiber structures in nanocomposites.					
33275405	1	16	theme	chitin	186:191	arg1	ChNFs					205:209	ChNFs	205:209	ChNFs	205:209	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	1	16	theme	chitin	186:191	arg1	nanofibers					193:202	chitin nanofibers	186:202	chitin nanofibers (ChNFs)	186:210	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	0	17	theme	Nanofiber	54:62	arg1	Mixtures					64:71	Nanofiber Mixtures	54:71	Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers	54:119	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.					
33275405	5	18	theme	scattering	924:933	arg1	studies					935:941	light scattering studies	918:941	light scattering studies	918:941	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	4	19	theme	mechanical	787:796	arg1	performance					798:808	promising mechanical performance	777:808	promising mechanical performance	777:808	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	1	20	theme	nanofibers	193:202	arg1	incorporation					135:147	Simultaneous incorporation	122:147	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix	122:248	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	5	21	theme	interfacial	1049:1059	arg1	interactions					1061:1072	the stronger interfacial interactions	1036:1072	the stronger interfacial interactions between CNCs and PVA	1036:1093	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	5	22	theme	CNC/ChNF	960:967	arg1	structures					969:978	the CNC/ChNF structures	956:978	the CNC/ChNF structures	956:978	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	6	23	from	structures	1235:1244	arg1	nanocomposites					1249:1262	nanocomposites	1249:1262	nanocomposites	1249:1262	Overall, these results provide insight into using electrostatically driven nanofiber structures in nanocomposites.					
33275405	2	24	theme	surface	402:408	arg1	charges					410:416	the opposite surface charges	389:416	the opposite surface charges on CNCs and ChNFs	389:434	When used in tricomponent composite hydrogels, the opposite surface charges on CNCs and ChNFs lead to the construction of beneficial nanofiber structures.					
33275405	0	25	theme	Synergistic	0:10	arg1	Reinforcement					12:24	Synergistic Reinforcement	0:24	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.	0:120	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.					
33275405	5	26	from	studies	935:941	arg1	results					870:876	results	870:876	results from water absorption, rheological, and light scattering studies	870:941	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	4	27	theme	CNCs/ChNFs	748:757	arg1	ratio					739:743	a specific ratio	728:743	a specific ratio of CNCs/ChNFs	728:757	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	2	28	theme	opposite	393:400	arg1	charges					410:416	the opposite surface charges	389:416	the opposite surface charges on CNCs and ChNFs	389:434	When used in tricomponent composite hydrogels, the opposite surface charges on CNCs and ChNFs lead to the construction of beneficial nanofiber structures.					
33275405	0	29	theme	Composite	29:37	arg1	Hydrogels					39:47	Composite Hydrogels	29:47	Composite Hydrogels	29:47	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.					
33275405	4	30	theme	other	827:831	arg1	samples					833:839	other samples	827:839	other samples	827:839	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	1	31	theme	Simultaneous	122:133	arg1	incorporation					135:147	Simultaneous incorporation	122:147	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix	122:248	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	3	32	theme	freeze-thaw	599:609	arg1	treatments					616:625	cyclic freeze-thaw (FT) treatments	592:625	cyclic freeze-thaw (FT) treatments	592:625	In this work, composite hydrogels containing CNCs, ChNFs, or their mixtures are produced using cyclic freeze-thaw (FT) treatments.					
33275405	0	33	theme	Hydrogels	39:47	arg1	Reinforcement					12:24	Synergistic Reinforcement	0:24	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.	0:120	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.					
33275405	2	34	theme	structures	485:494	arg1	construction					448:459	the construction	444:459	the construction of beneficial nanofiber structures	444:494	When used in tricomponent composite hydrogels, the opposite surface charges on CNCs and ChNFs lead to the construction of beneficial nanofiber structures.					
33275405	5	35	theme	ChNFs	1125:1129	arg1	interactions					1061:1072	the stronger interfacial interactions	1036:1072	the stronger interfacial interactions between CNCs and PVA	1036:1093	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	5	35	theme	ChNFs	1125:1129	arg1	lengths					1110:1116	the longer lengths	1099:1116	the longer lengths of the ChNFs for load transfer	1099:1147	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	1	36	theme	polyvinyl	219:227	arg1	PVA					238:240	PVA	238:240	PVA	238:240	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	1	36	theme	polyvinyl	219:227	arg1	alcohol					229:235	a polyvinyl alcohol	217:235	a polyvinyl alcohol (PVA) matrix	217:248	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	2	37	theme	nanofiber	475:483	arg1	structures					485:494	beneficial nanofiber structures	464:494	beneficial nanofiber structures	464:494	When used in tricomponent composite hydrogels, the opposite surface charges on CNCs and ChNFs lead to the construction of beneficial nanofiber structures.					
33275405	1	38	theme	composite	321:329	arg1	materials					331:339	more environmentally friendly composite materials	291:339	more environmentally friendly composite materials	291:339	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	2	39	theme	tricomponent	355:366	arg1	hydrogels					378:386	tricomponent composite hydrogels	355:386	tricomponent composite hydrogels	355:386	When used in tricomponent composite hydrogels, the opposite surface charges on CNCs and ChNFs lead to the construction of beneficial nanofiber structures.					
33275405	2	40	theme	beneficial	464:473	arg1	structures					485:494	beneficial nanofiber structures	464:494	beneficial nanofiber structures	464:494	When used in tricomponent composite hydrogels, the opposite surface charges on CNCs and ChNFs lead to the construction of beneficial nanofiber structures.					
33275405	1	41	theme	materials	331:339	arg1	customization					274:286	customization	274:286	customization of more environmentally friendly composite materials	274:339	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	3	42	theme	cyclic	592:597	arg1	treatments					616:625	cyclic freeze-thaw (FT) treatments	592:625	cyclic freeze-thaw (FT) treatments	592:625	In this work, composite hydrogels containing CNCs, ChNFs, or their mixtures are produced using cyclic freeze-thaw (FT) treatments.					
33275405	0	43	with	Reinforcement	12:24	arg1	Mixtures					64:71	Nanofiber Mixtures	54:71	Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers	54:119	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.					
33275405	2	44	from	charges	410:416	arg1	ChNFs					430:434	ChNFs	430:434	ChNFs	430:434	When used in tricomponent composite hydrogels, the opposite surface charges on CNCs and ChNFs lead to the construction of beneficial nanofiber structures.					
33275405	2	44	from	charges	410:416	arg1	CNCs					421:424	CNCs	421:424	CNCs	421:424	When used in tricomponent composite hydrogels, the opposite surface charges on CNCs and ChNFs lead to the construction of beneficial nanofiber structures.					
33275405	1	45	theme	cellulose	152:160	arg1	CNCs					176:179	CNCs	176:179	CNCs	176:179	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	1	45	theme	cellulose	152:160	arg1	nanocrystals					162:173	cellulose nanocrystals	152:173	cellulose nanocrystals (CNCs)	152:180	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	1	46	theme	alcohol	229:235	arg1	matrix					243:248	a polyvinyl alcohol (PVA) matrix	217:248	a polyvinyl alcohol (PVA) matrix	217:248	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	6	47	theme	driven	1218:1223	arg1	structures					1235:1244	electrostatically driven nanofiber structures	1200:1244	electrostatically driven nanofiber structures in nanocomposites	1200:1262	Overall, these results provide insight into using electrostatically driven nanofiber structures in nanocomposites.					
33275405	5	48	theme	longer	1103:1108	arg1	lengths					1110:1116	the longer lengths	1099:1116	the longer lengths of the ChNFs for load transfer	1099:1147	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	5	49	theme	stronger	1040:1047	arg1	interactions					1061:1072	the stronger interfacial interactions	1036:1072	the stronger interfacial interactions between CNCs and PVA	1036:1093	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	1	50	theme	nanocrystals	162:173	arg1	incorporation					135:147	Simultaneous incorporation	122:147	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix	122:248	Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials.					
33275405	0	51	theme	Cellulose	76:84	arg1	Nanocrystals					86:97	Cellulose Nanocrystals	76:97	Cellulose Nanocrystals	76:97	Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.					
33275405	4	52	theme	different	645:653	arg1	compositions					655:666	different compositions	645:666	different compositions	645:666	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	3	53	theme	FT	612:613	arg1	treatments					616:625	cyclic freeze-thaw (FT) treatments	592:625	cyclic freeze-thaw (FT) treatments	592:625	In this work, composite hydrogels containing CNCs, ChNFs, or their mixtures are produced using cyclic freeze-thaw (FT) treatments.					
33275405	5	54	from	rheological	901:911	arg1	results					870:876	results	870:876	results from water absorption, rheological, and light scattering studies	870:941	These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer.					
33275405	4	55	theme	composite	697:705	arg1	hydrogels					707:715	tricomponent composite hydrogels	684:715	tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs	684:757	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
33275405	4	56	theme	tricomponent	684:695	arg1	hydrogels					707:715	tricomponent composite hydrogels	684:715	tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs	684:757	When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples.					
32797646	2	0	theme	sugars	440:445	arg1	biosynthesis					416:427	the biosynthesis	412:427	the biosynthesis of unusual sugars	412:445	Remarkably, this virus, known as Mimivirus, has a genome that encodes for nearly 1,000 proteins, some of which are involved in the biosynthesis of unusual sugars.					
32797646	0	1	from	structure	20:28	arg1	polyphaga					77:85	Acanthamoeba polyphaga	64:85	Acanthamoeba polyphaga	64:85	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga Mimivirus.					
32797646	1	2	theme	most	144:147	arg1	events					167:172	the most paradigm-shifting events	140:172	the most paradigm-shifting events	140:172	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	1	3	theme	giant	231:235	arg1	virus					257:261	the giant double-stranded DNA virus	227:261	the giant double-stranded DNA virus that infects amoebae	227:282	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	2	4	theme	unusual	432:438	arg1	sugars					440:445	unusual sugars	432:445	unusual sugars	432:445	Remarkably, this virus, known as Mimivirus, has a genome that encodes for nearly 1,000 proteins, some of which are involved in the biosynthesis of unusual sugars.					
32797646	9	5	theme	key	1485:1487	arg1	roles					1489:1493	key roles	1485:1493	key roles	1485:1493	Site-directed mutagenesis experiments suggest that both Cys 108 and Lys 175 play key roles in catalysis.					
32797646	1	6	theme	paradigm-shifting	149:165	arg1	events					167:172	the most paradigm-shifting events	140:172	the most paradigm-shifting events	140:172	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	1	7	theme	double-stranded	237:251	arg1	virus					257:261	the giant double-stranded DNA virus	227:261	the giant double-stranded DNA virus that infects amoebae	227:282	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	7	8	theme	dehydrogenase/reductase	1207:1229	arg1	superfamily					1231:1241	the short-chain dehydrogenase/reductase superfamily	1191:1241	the short-chain dehydrogenase/reductase superfamily	1191:1241	The overall molecular architecture of L780 places it into the short-chain dehydrogenase/reductase superfamily.					
32797646	4	9	theme	frame	719:723	arg1	L780					725:728	the open-reading frame L780	702:728	the open-reading frame L780	702:728	Here we describe a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase.					
32797646	1	10	theme	DNA	253:255	arg1	virus					257:261	the giant double-stranded DNA virus	227:261	the giant double-stranded DNA virus that infects amoebae	227:282	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	4	11	theme	open-reading	706:717	arg1	L780					725:728	the open-reading frame L780	702:728	the open-reading frame L780	702:728	Here we describe a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase.					
32797646	8	12	theme	catalytic	1352:1360	arg1	efficiency					1362:1371	a higher catalytic efficiency	1343:1371	a higher catalytic efficiency with the UDP-linked substrate	1343:1401	Kinetic analyses indicate that the enzyme can function on either UDP- and dTDP-sugars but displays a higher catalytic efficiency with the UDP-linked substrate.					
32797646	7	13	theme	overall	1137:1143	arg1	architecture					1155:1166	The overall molecular architecture	1133:1166	The overall molecular architecture of L780	1133:1174	The overall molecular architecture of L780 places it into the short-chain dehydrogenase/reductase superfamily.					
32797646	8	14	theme	higher	1345:1350	arg1	efficiency					1362:1371	a higher catalytic efficiency	1343:1371	a higher catalytic efficiency with the UDP-linked substrate	1343:1401	Kinetic analyses indicate that the enzyme can function on either UDP- and dTDP-sugars but displays a higher catalytic efficiency with the UDP-linked substrate.					
32797646	1	15	theme	virus	257:261	arg1	events					167:172	the most paradigm-shifting events	140:172	the most paradigm-shifting events	140:172	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	1	15	theme	virus	257:261	arg1	one					133:135	one	133:135	one	133:135	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	1	15	theme	virus	257:261	arg1	identification					209:222	the identification	205:222	the identification of the giant double-stranded DNA virus that infects amoebae	205:282	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	10	16	theme	new	1599:1601	arg1	details					1603:1609	new details	1599:1609	new details into these fascinating enzymes	1599:1640	This structure represents the first model of a viral UDP-l-rhamnose synthase and provides new details into these fascinating enzymes.					
32797646	2	17	contain	has	329:331	arg2	genome					335:340	a genome	333:340	a genome that encodes for nearly 1,000 proteins, some of which are involved in the biosynthesis of unusual sugars	333:445	Remarkably, this virus, known as Mimivirus, has a genome that encodes for nearly 1,000 proteins, some of which are involved in the biosynthesis of unusual sugars.					
32797646	2	17	contain	has	329:331	arg1	virus					302:306	this virus	297:306	this virus	297:306	Remarkably, this virus, known as Mimivirus, has a genome that encodes for nearly 1,000 proteins, some of which are involved in the biosynthesis of unusual sugars.					
32797646	5	18	theme	/UDP-l-rhamnose	808:822	arg1	complex					832:838	the L780/NADP+ /UDP-l-rhamnose ternary complex	793:838	the L780/NADP+ /UDP-l-rhamnose ternary complex	793:838	The structure of the L780/NADP+ /UDP-l-rhamnose ternary complex was determined to 1.45 Å resolution and refined to an overall R-factor of 19.9%.					
32797646	1	19	theme	events	167:172	arg1	events					167:172	the most paradigm-shifting events	140:172	the most paradigm-shifting events	140:172	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	1	19	theme	events	167:172	arg1	one					133:135	one	133:135	one	133:135	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	1	19	theme	events	167:172	arg1	identification					209:222	the identification	205:222	the identification of the giant double-stranded DNA virus that infects amoebae	205:282	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	6	20	theme	protein	949:955	arg1	subunit					926:932	Each subunit	921:932	Each subunit of the dimeric protein	921:955	Each subunit of the dimeric protein adopts a bilobal-shaped appearance with the N-terminal domain harboring the dinucleotide-binding site and the C-terminal domain positioning the UDP-sugar into the active site.					
32797646	6	21	theme	bilobal-shaped	966:979	arg1	appearance					981:990	a bilobal-shaped appearance	964:990	a bilobal-shaped appearance with the N-terminal domain harboring the dinucleotide-binding site and the C-terminal domain positioning the UDP-sugar into the active site	964:1130	Each subunit of the dimeric protein adopts a bilobal-shaped appearance with the N-terminal domain harboring the dinucleotide-binding site and the C-terminal domain positioning the UDP-sugar into the active site.					
32797646	0	22	theme	high-resolution	4:18	arg1	structure					20:28	The high-resolution structure	0:28	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga	0:85	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga Mimivirus.					
32797646	10	23	theme	viral	1556:1560	arg1	synthase					1577:1584	a viral UDP-l-rhamnose synthase	1554:1584	a viral UDP-l-rhamnose synthase	1554:1584	This structure represents the first model of a viral UDP-l-rhamnose synthase and provides new details into these fascinating enzymes.					
32797646	1	24	from	one	133:135	arg1	century					193:199	the 21st century	184:199	the 21st century	184:199	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	3	25	theme	fibers	503:508	arg1	layer					481:485	a layer	479:485	a layer of glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose	479:602	Indeed, the virus is coated by a layer of glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose.					
32797646	4	26	theme	structural	633:642	arg1	investigation					662:674	a combined structural and enzymological investigation	622:674	a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase	622:773	Here we describe a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase.					
32797646	6	27	theme	dimeric	941:947	arg1	protein					949:955	the dimeric protein	937:955	the dimeric protein	937:955	Each subunit of the dimeric protein adopts a bilobal-shaped appearance with the N-terminal domain harboring the dinucleotide-binding site and the C-terminal domain positioning the UDP-sugar into the active site.					
32797646	0	28	dep	Mimivirus	87:95	arg1	structure					20:28	The high-resolution structure	0:28	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga	0:85	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga Mimivirus.					
32797646	4	29	theme	combined	624:631	arg1	investigation					662:674	a combined structural and enzymological investigation	622:674	a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase	622:773	Here we describe a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase.					
32797646	10	30	theme	fascinating	1622:1632	arg1	enzymes					1634:1640	these fascinating enzymes	1616:1640	these fascinating enzymes	1616:1640	This structure represents the first model of a viral UDP-l-rhamnose synthase and provides new details into these fascinating enzymes.					
32797646	9	31	theme	Site-directed	1404:1416	arg1	experiments					1430:1440	Site-directed mutagenesis experiments	1404:1440	Site-directed mutagenesis experiments	1404:1440	Site-directed mutagenesis experiments suggest that both Cys 108 and Lys 175 play key roles in catalysis.					
32797646	5	32	theme	L780/NADP+	797:806	arg1	complex					832:838	the L780/NADP+ /UDP-l-rhamnose ternary complex	793:838	the L780/NADP+ /UDP-l-rhamnose ternary complex	793:838	The structure of the L780/NADP+ /UDP-l-rhamnose ternary complex was determined to 1.45 Å resolution and refined to an overall R-factor of 19.9%.					
32797646	5	33	theme	%	918:918	arg1	R-factor					902:909	an overall R-factor	891:909	an overall R-factor of 19.9%	891:918	The structure of the L780/NADP+ /UDP-l-rhamnose ternary complex was determined to 1.45 Å resolution and refined to an overall R-factor of 19.9%.					
32797646	3	34	theme	glycosylated	490:501	arg1	fibers					503:508	glycosylated fibers	490:508	glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose	490:602	Indeed, the virus is coated by a layer of glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose.					
32797646	7	35	theme	molecular	1145:1153	arg1	architecture					1155:1166	The overall molecular architecture	1133:1166	The overall molecular architecture of L780	1133:1174	The overall molecular architecture of L780 places it into the short-chain dehydrogenase/reductase superfamily.					
32797646	1	36	theme	21st	188:191	arg1	century					193:199	the 21st century	184:199	the 21st century	184:199	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	0	37	theme	UDP-L-rhamnose	35:48	arg1	synthase					50:57	a UDP-L-rhamnose synthase	33:57	a UDP-L-rhamnose synthase from Acanthamoeba polyphaga	33:85	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga Mimivirus.					
32797646	6	38	theme	active	1120:1125	arg1	site					1127:1130	the active site	1116:1130	the active site	1116:1130	Each subunit of the dimeric protein adopts a bilobal-shaped appearance with the N-terminal domain harboring the dinucleotide-binding site and the C-terminal domain positioning the UDP-sugar into the active site.					
32797646	5	39	theme	1.45 Å	858:863	arg1	resolution					865:874	1.45 Å resolution	858:874	1.45 Å resolution	858:874	The structure of the L780/NADP+ /UDP-l-rhamnose ternary complex was determined to 1.45 Å resolution and refined to an overall R-factor of 19.9%.					
32797646	5	40	theme	ternary	824:830	arg1	complex					832:838	the L780/NADP+ /UDP-l-rhamnose ternary complex	793:838	the L780/NADP+ /UDP-l-rhamnose ternary complex	793:838	The structure of the L780/NADP+ /UDP-l-rhamnose ternary complex was determined to 1.45 Å resolution and refined to an overall R-factor of 19.9%.					
32797646	6	41	theme	C-terminal	1067:1076	arg1	domain					1078:1083	the C-terminal domain	1063:1083	the C-terminal domain positioning the UDP-sugar into the active site	1063:1130	Each subunit of the dimeric protein adopts a bilobal-shaped appearance with the N-terminal domain harboring the dinucleotide-binding site and the C-terminal domain positioning the UDP-sugar into the active site.					
32797646	3	42	contain	contain	515:521	arg2	N-acetyl-d-glucosamine					534:555	N-acetyl-d-glucosamine	534:555	N-acetyl-d-glucosamine	534:555	Indeed, the virus is coated by a layer of glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose.					
32797646	3	42	contain	contain	515:521	arg2	l-rhamnose					558:567	l-rhamnose	558:567	l-rhamnose	558:567	Indeed, the virus is coated by a layer of glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose.					
32797646	3	42	contain	contain	515:521	arg1	fibers					503:508	glycosylated fibers	490:508	glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose	490:602	Indeed, the virus is coated by a layer of glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose.					
32797646	3	42	contain	contain	515:521	arg2	d-glucose					523:531	d-glucose	523:531	d-glucose	523:531	Indeed, the virus is coated by a layer of glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose.					
32797646	3	42	contain	contain	515:521	arg2	4-amino-4,6-dideoxy-d-glucose					574:602	4-amino-4,6-dideoxy-d-glucose	574:602	4-amino-4,6-dideoxy-d-glucose	574:602	Indeed, the virus is coated by a layer of glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose.					
32797646	0	43	from	polyphaga	77:85	arg1	structure					20:28	The high-resolution structure	0:28	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga	0:85	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga Mimivirus.					
32797646	0	43	from	polyphaga	77:85	arg1	synthase					50:57	a UDP-L-rhamnose synthase	33:57	a UDP-L-rhamnose synthase from Acanthamoeba polyphaga	33:85	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga Mimivirus.					
32797646	6	44	with	appearance	981:990	arg1	domain					1078:1083	the C-terminal domain	1063:1083	the C-terminal domain positioning the UDP-sugar into the active site	1063:1130	Each subunit of the dimeric protein adopts a bilobal-shaped appearance with the N-terminal domain harboring the dinucleotide-binding site and the C-terminal domain positioning the UDP-sugar into the active site.					
32797646	6	44	with	appearance	981:990	arg1	domain					1012:1017	the N-terminal domain	997:1017	the N-terminal domain harboring the dinucleotide-binding site	997:1057	Each subunit of the dimeric protein adopts a bilobal-shaped appearance with the N-terminal domain harboring the dinucleotide-binding site and the C-terminal domain positioning the UDP-sugar into the active site.					
32797646	5	45	theme	complex	832:838	arg1	structure					780:788	The structure	776:788	The structure of the L780/NADP+ /UDP-l-rhamnose ternary complex	776:838	The structure of the L780/NADP+ /UDP-l-rhamnose ternary complex was determined to 1.45 Å resolution and refined to an overall R-factor of 19.9%.					
32797646	8	46	theme	Kinetic	1244:1250	arg1	analyses					1252:1259	Kinetic analyses	1244:1259	Kinetic analyses	1244:1259	Kinetic analyses indicate that the enzyme can function on either UDP- and dTDP-sugars but displays a higher catalytic efficiency with the UDP-linked substrate.					
32797646	10	47	theme	synthase	1577:1584	arg1	model					1545:1549	the first model	1535:1549	the first model of a viral UDP-l-rhamnose synthase	1535:1584	This structure represents the first model of a viral UDP-l-rhamnose synthase and provides new details into these fascinating enzymes.					
32797646	8	48	theme	UDP-linked	1382:1391	arg1	substrate					1393:1401	the UDP-linked substrate	1378:1401	the UDP-linked substrate	1378:1401	Kinetic analyses indicate that the enzyme can function on either UDP- and dTDP-sugars but displays a higher catalytic efficiency with the UDP-linked substrate.					
32797646	1	49	theme	virology	115:122	arg1	field					106:110	the field	102:110	the field of virology	102:122	For the field of virology, perhaps one of the most paradigm-shifting events so far in the 21st century was the identification of the giant double-stranded DNA virus that infects amoebae.					
32797646	0	50	theme	synthase	50:57	arg1	structure					20:28	The high-resolution structure	0:28	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga	0:85	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga Mimivirus.					
32797646	10	51	theme	UDP-l-rhamnose	1562:1575	arg1	synthase					1577:1584	a viral UDP-l-rhamnose synthase	1554:1584	a viral UDP-l-rhamnose synthase	1554:1584	This structure represents the first model of a viral UDP-l-rhamnose synthase and provides new details into these fascinating enzymes.					
32797646	5	52	theme	overall	894:900	arg1	R-factor					902:909	an overall R-factor	891:909	an overall R-factor of 19.9%	891:918	The structure of the L780/NADP+ /UDP-l-rhamnose ternary complex was determined to 1.45 Å resolution and refined to an overall R-factor of 19.9%.					
32797646	8	53	link	UDP-linked	1382:1391	arg1	substrate					1393:1401	the UDP-linked substrate	1378:1401	the UDP-linked substrate	1378:1401	Kinetic analyses indicate that the enzyme can function on either UDP- and dTDP-sugars but displays a higher catalytic efficiency with the UDP-linked substrate.					
32797646	4	54	theme	protein	683:689	arg1	investigation					662:674	a combined structural and enzymological investigation	622:674	a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase	622:773	Here we describe a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase.					
32797646	4	55	theme	l-rhamnose	755:764	arg1	synthase					766:773	an l-rhamnose synthase	752:773	an l-rhamnose synthase	752:773	Here we describe a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase.					
32797646	3	56	gly	glycosylated	490:501	arg1	fibers					503:508	glycosylated fibers	490:508	glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose	490:602	Indeed, the virus is coated by a layer of glycosylated fibers that contain d-glucose, N-acetyl-d-glucosamine, l-rhamnose, and 4-amino-4,6-dideoxy-d-glucose.					
32797646	9	57	theme	mutagenesis	1418:1428	arg1	experiments					1430:1440	Site-directed mutagenesis experiments	1404:1440	Site-directed mutagenesis experiments	1404:1440	Site-directed mutagenesis experiments suggest that both Cys 108 and Lys 175 play key roles in catalysis.					
32797646	6	58	theme	N-terminal	1001:1010	arg1	domain					1012:1017	the N-terminal domain	997:1017	the N-terminal domain harboring the dinucleotide-binding site	997:1057	Each subunit of the dimeric protein adopts a bilobal-shaped appearance with the N-terminal domain harboring the dinucleotide-binding site and the C-terminal domain positioning the UDP-sugar into the active site.					
32797646	10	59	theme	first	1539:1543	arg1	model					1545:1549	the first model	1535:1549	the first model of a viral UDP-l-rhamnose synthase	1535:1584	This structure represents the first model of a viral UDP-l-rhamnose synthase and provides new details into these fascinating enzymes.					
32797646	6	60	theme	dinucleotide-binding	1033:1052	arg1	site					1054:1057	the dinucleotide-binding site	1029:1057	the dinucleotide-binding site	1029:1057	Each subunit of the dimeric protein adopts a bilobal-shaped appearance with the N-terminal domain harboring the dinucleotide-binding site and the C-terminal domain positioning the UDP-sugar into the active site.					
32797646	4	61	theme	enzymological	648:660	arg1	investigation					662:674	a combined structural and enzymological investigation	622:674	a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase	622:773	Here we describe a combined structural and enzymological investigation of the protein encoded by the open-reading frame L780, which corresponds to an l-rhamnose synthase.					
32797646	8	62	with	efficiency	1362:1371	arg1	substrate					1393:1401	the UDP-linked substrate	1378:1401	the UDP-linked substrate	1378:1401	Kinetic analyses indicate that the enzyme can function on either UDP- and dTDP-sugars but displays a higher catalytic efficiency with the UDP-linked substrate.					
32797646	0	63	theme	Acanthamoeba	64:75	arg1	polyphaga					77:85	Acanthamoeba polyphaga	64:85	Acanthamoeba polyphaga	64:85	The high-resolution structure of a UDP-L-rhamnose synthase from Acanthamoeba polyphaga Mimivirus.					
32797646	7	64	theme	L780	1171:1174	arg1	architecture					1155:1166	The overall molecular architecture	1133:1166	The overall molecular architecture of L780	1133:1174	The overall molecular architecture of L780 places it into the short-chain dehydrogenase/reductase superfamily.					
32797646	7	65	theme	short-chain	1195:1205	arg1	superfamily					1231:1241	the short-chain dehydrogenase/reductase superfamily	1191:1241	the short-chain dehydrogenase/reductase superfamily	1191:1241	The overall molecular architecture of L780 places it into the short-chain dehydrogenase/reductase superfamily.					
34420748	6	0	theme	elastic	912:918	arg1	feature					920:926	their elastic feature	906:926	their elastic feature	906:926	Further, the addition of TA in CF hydrogels improved their elastic feature, inhibited inflammatory response, and promoted the formation of mature and well-organized collagen fibers.					
34420748	5	1	theme	adhesion	832:839	arg1	properties					841:850	adhesion properties	832:850	adhesion properties	832:850	Incorporating fucoidan in chitosan hydrogels greatly enhanced their swelling behavior, mechanical strength, and adhesion properties.					
34420748	8	2	theme	designed	1184:1191	arg1	hydrogels					1193:1201	the designed hydrogels	1180:1201	the designed hydrogels	1180:1201	Thus, the designed hydrogels allow the development of oral mucosa patches as a potential treatment for OIDs.					
34420748	1	3	theme	human	241:245	arg1	life					247:250	human life	241:250	human life	241:250	Oral inflammatory diseases (OIDs) are among the most common lesions in the oral cavity, affecting the quality of human life and even causing oral cancer.					
34420748	0	4	theme	mucosa	114:119	arg1	patch					121:125	an oral mucosa patch	106:125	an oral mucosa patch	106:125	Preparation of triamcinolone acetonide-loaded chitosan/fucoidan hydrogel and its potential application as an oral mucosa patch.					
34420748	7	5	theme	good	1127:1130	arg1	cytocompatibility					1132:1148	cytocompatibility	1132:1148	cytocompatibility	1132:1148	The developed composite hydrogels displayed not only good antibacterial properties but also good cytocompatibility and histocompatibility.					
34420748	2	6	dep	patches	323:329	arg1	have					337:340	have	337:340	patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation	323:476	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	1	7	theme	life	247:250	arg1	quality					230:236	the quality	226:236	the quality of human life	226:250	Oral inflammatory diseases (OIDs) are among the most common lesions in the oral cavity, affecting the quality of human life and even causing oral cancer.					
34420748	0	8	theme	oral	109:112	arg1	patch					121:125	an oral mucosa patch	106:125	an oral mucosa patch	106:125	Preparation of triamcinolone acetonide-loaded chitosan/fucoidan hydrogel and its potential application as an oral mucosa patch.					
34420748	1	9	theme	Oral	128:131	arg1	diseases					146:153	Oral inflammatory diseases	128:153	Oral inflammatory diseases (OIDs)	128:160	Oral inflammatory diseases (OIDs) are among the most common lesions in the oral cavity, affecting the quality of human life and even causing oral cancer.					
34420748	1	9	theme	Oral	128:131	arg1	OIDs					156:159	OIDs	156:159	OIDs	156:159	Oral inflammatory diseases (OIDs) are among the most common lesions in the oral cavity, affecting the quality of human life and even causing oral cancer.					
34420748	3	10	theme	triamcinolone	487:499	arg1	TA					512:513	TA	512:513	TA	512:513	Herein, triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels were prepared via chemical crosslinking.					
34420748	3	10	theme	triamcinolone	487:499	arg1	acetonide					501:509	triamcinolone acetonide	487:509	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	Herein, triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels were prepared via chemical crosslinking.					
34420748	4	11	theme	bio	647:649	arg1	properties					667:676	(bio)physicochemical properties	646:676	(bio)physicochemical properties	646:676	The macro/microscopic morphologies and (bio)physicochemical properties of composite hydrogels were investigated.					
34420748	3	12	theme	acetonide	501:509	arg1	hydrogels					556:564	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	Herein, triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels were prepared via chemical crosslinking.					
34420748	5	13	theme	mechanical	807:816	arg1	strength					818:825	mechanical strength	807:825	mechanical strength	807:825	Incorporating fucoidan in chitosan hydrogels greatly enhanced their swelling behavior, mechanical strength, and adhesion properties.					
34420748	6	14	theme	TA	878:879	arg1	addition					866:873	the addition	862:873	the addition of TA in CF hydrogels	862:895	Further, the addition of TA in CF hydrogels improved their elastic feature, inhibited inflammatory response, and promoted the formation of mature and well-organized collagen fibers.					
34420748	7	15	theme	antibacterial	1093:1105	arg1	properties					1107:1116	good antibacterial properties	1088:1116	not only good antibacterial properties but also good cytocompatibility and histocompatibility	1079:1171	The developed composite hydrogels displayed not only good antibacterial properties but also good cytocompatibility and histocompatibility.					
34420748	5	16	theme	chitosan	746:753	arg1	hydrogels					755:763	chitosan hydrogels	746:763	chitosan hydrogels	746:763	Incorporating fucoidan in chitosan hydrogels greatly enhanced their swelling behavior, mechanical strength, and adhesion properties.					
34420748	3	17	theme	chitosan/fucoidan	523:539	arg1	hydrogels					556:564	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	Herein, triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels were prepared via chemical crosslinking.					
34420748	6	18	theme	CF	884:885	arg1	hydrogels					887:895	CF hydrogels	884:895	CF hydrogels	884:895	Further, the addition of TA in CF hydrogels improved their elastic feature, inhibited inflammatory response, and promoted the formation of mature and well-organized collagen fibers.					
34420748	1	19	theme	common	181:186	arg1	lesions					188:194	the most common lesions	172:194	the most common lesions in the oral cavity	172:213	Oral inflammatory diseases (OIDs) are among the most common lesions in the oral cavity, affecting the quality of human life and even causing oral cancer.					
34420748	0	20	theme	acetonide-loaded	29:44	arg1	hydrogel					64:71	triamcinolone acetonide-loaded chitosan/fucoidan hydrogel	15:71	triamcinolone acetonide-loaded chitosan/fucoidan hydrogel	15:71	Preparation of triamcinolone acetonide-loaded chitosan/fucoidan hydrogel and its potential application as an oral mucosa patch.					
34420748	2	21	theme	mechanical	387:396	arg1	strength					398:405	poor mechanical strength	382:405	poor mechanical strength	382:405	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	2	21	theme	mechanical	387:396	arg1	limitations					347:357	some limitations	342:357	some limitations	342:357	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	0	22	theme	triamcinolone	15:27	arg1	hydrogel					64:71	triamcinolone acetonide-loaded chitosan/fucoidan hydrogel	15:71	triamcinolone acetonide-loaded chitosan/fucoidan hydrogel	15:71	Preparation of triamcinolone acetonide-loaded chitosan/fucoidan hydrogel and its potential application as an oral mucosa patch.					
34420748	1	23	theme	oral	269:272	arg1	cancer					274:279	oral cancer	269:279	oral cancer	269:279	Oral inflammatory diseases (OIDs) are among the most common lesions in the oral cavity, affecting the quality of human life and even causing oral cancer.					
34420748	7	24	theme	good	1088:1091	arg1	properties					1107:1116	good antibacterial properties	1088:1116	not only good antibacterial properties but also good cytocompatibility and histocompatibility	1079:1171	The developed composite hydrogels displayed not only good antibacterial properties but also good cytocompatibility and histocompatibility.					
34420748	3	25	theme	-loaded	515:521	arg1	hydrogels					556:564	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	Herein, triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels were prepared via chemical crosslinking.					
34420748	2	26	theme	poor	382:385	arg1	strength					398:405	poor mechanical strength	382:405	poor mechanical strength	382:405	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	2	26	theme	poor	382:385	arg1	limitations					347:357	some limitations	342:357	some limitations	342:357	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	0	27	theme	hydrogel	64:71	arg1	Preparation					0:10	Preparation	0:10	Preparation of triamcinolone acetonide-loaded chitosan/fucoidan hydrogel	0:71	Preparation of triamcinolone acetonide-loaded chitosan/fucoidan hydrogel and its potential application as an oral mucosa patch.					
34420748	0	27	theme	hydrogel	64:71	arg1	application					91:101	its potential application	77:101	its potential application as an oral mucosa patch	77:125	Preparation of triamcinolone acetonide-loaded chitosan/fucoidan hydrogel and its potential application as an oral mucosa patch.					
34420748	2	28	theme	current	303:309	arg1	mucosa					316:321	the current oral mucosa	299:321	the current oral mucosa	299:321	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	4	29	theme	macro/microscopic	611:627	arg1	morphologies					629:640	The macro/microscopic morphologies	607:640	The macro/microscopic morphologies	607:640	The macro/microscopic morphologies and (bio)physicochemical properties of composite hydrogels were investigated.					
34420748	3	30	theme	composite	546:554	arg1	hydrogels					556:564	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	Herein, triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels were prepared via chemical crosslinking.					
34420748	8	31	theme	oral	1228:1231	arg1	patches					1240:1246	oral mucosa patches	1228:1246	oral mucosa patches	1228:1246	Thus, the designed hydrogels allow the development of oral mucosa patches as a potential treatment for OIDs.					
34420748	1	32	theme	oral	203:206	arg1	cavity					208:213	the oral cavity	199:213	the oral cavity	199:213	Oral inflammatory diseases (OIDs) are among the most common lesions in the oral cavity, affecting the quality of human life and even causing oral cancer.					
34420748	0	33	theme	chitosan/fucoidan	46:62	arg1	hydrogel					64:71	triamcinolone acetonide-loaded chitosan/fucoidan hydrogel	15:71	triamcinolone acetonide-loaded chitosan/fucoidan hydrogel	15:71	Preparation of triamcinolone acetonide-loaded chitosan/fucoidan hydrogel and its potential application as an oral mucosa patch.					
34420748	5	34	theme	swelling	788:795	arg1	behavior					797:804	their swelling behavior	782:804	their swelling behavior	782:804	Incorporating fucoidan in chitosan hydrogels greatly enhanced their swelling behavior, mechanical strength, and adhesion properties.					
34420748	6	35	theme	mature	992:997	arg1	fibers					1027:1032	mature and well-organized collagen fibers	992:1032	mature and well-organized collagen fibers	992:1032	Further, the addition of TA in CF hydrogels improved their elastic feature, inhibited inflammatory response, and promoted the formation of mature and well-organized collagen fibers.					
34420748	8	36	theme	mucosa	1233:1238	arg1	patches					1240:1246	oral mucosa patches	1228:1246	oral mucosa patches	1228:1246	Thus, the designed hydrogels allow the development of oral mucosa patches as a potential treatment for OIDs.					
34420748	8	37	theme	potential	1253:1261	arg1	treatment					1263:1271	a potential treatment	1251:1271	a potential treatment for OIDs	1251:1280	Thus, the designed hydrogels allow the development of oral mucosa patches as a potential treatment for OIDs.					
34420748	8	37	theme	potential	1253:1261	arg1	development					1213:1223	the development	1209:1223	the development of oral mucosa patches	1209:1246	Thus, the designed hydrogels allow the development of oral mucosa patches as a potential treatment for OIDs.					
34420748	6	38	theme	inflammatory	939:950	arg1	response					952:959	inflammatory response	939:959	inflammatory response	939:959	Further, the addition of TA in CF hydrogels improved their elastic feature, inhibited inflammatory response, and promoted the formation of mature and well-organized collagen fibers.					
34420748	7	39	theme	composite	1049:1057	arg1	hydrogels					1059:1067	The developed composite hydrogels	1035:1067	The developed composite hydrogels	1035:1067	The developed composite hydrogels displayed not only good antibacterial properties but also good cytocompatibility and histocompatibility.					
34420748	2	40	theme	foreign	455:461	arg1	limitations					347:357	some limitations	342:357	some limitations	342:357	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	2	40	theme	foreign	455:461	arg1	sensation					468:476	foreign body sensation	455:476	foreign body sensation	455:476	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	3	41	theme	CF	542:543	arg1	hydrogels					556:564	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels	487:564	Herein, triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels were prepared via chemical crosslinking.					
34420748	2	42	theme	oral	311:314	arg1	mucosa					316:321	the current oral mucosa	299:321	the current oral mucosa	299:321	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	2	43	theme	low	427:429	arg1	adhesion					431:438	low adhesion	427:438	low adhesion to tissue	427:448	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	2	43	theme	low	427:429	arg1	limitations					347:357	some limitations	342:357	some limitations	342:357	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	8	44	theme	patches	1240:1246	arg1	development					1213:1223	the development	1209:1223	the development of oral mucosa patches	1209:1246	Thus, the designed hydrogels allow the development of oral mucosa patches as a potential treatment for OIDs.					
34420748	8	44	theme	patches	1240:1246	arg1	treatment					1263:1271	a potential treatment	1251:1271	a potential treatment for OIDs	1251:1280	Thus, the designed hydrogels allow the development of oral mucosa patches as a potential treatment for OIDs.					
34420748	1	45	theme	inflammatory	133:144	arg1	diseases					146:153	Oral inflammatory diseases	128:153	Oral inflammatory diseases (OIDs)	128:160	Oral inflammatory diseases (OIDs) are among the most common lesions in the oral cavity, affecting the quality of human life and even causing oral cancer.					
34420748	1	45	theme	inflammatory	133:144	arg1	OIDs					156:159	OIDs	156:159	OIDs	156:159	Oral inflammatory diseases (OIDs) are among the most common lesions in the oral cavity, affecting the quality of human life and even causing oral cancer.					
34420748	4	46	theme	hydrogels	691:699	arg1	properties					667:676	(bio)physicochemical properties	646:676	(bio)physicochemical properties	646:676	The macro/microscopic morphologies and (bio)physicochemical properties of composite hydrogels were investigated.					
34420748	4	46	theme	hydrogels	691:699	arg1	morphologies					629:640	The macro/microscopic morphologies	607:640	The macro/microscopic morphologies	607:640	The macro/microscopic morphologies and (bio)physicochemical properties of composite hydrogels were investigated.					
34420748	6	47	from	addition	866:873	arg1	hydrogels					887:895	CF hydrogels	884:895	CF hydrogels	884:895	Further, the addition of TA in CF hydrogels improved their elastic feature, inhibited inflammatory response, and promoted the formation of mature and well-organized collagen fibers.					
34420748	7	48	theme	developed	1039:1047	arg1	hydrogels					1059:1067	The developed composite hydrogels	1035:1067	The developed composite hydrogels	1035:1067	The developed composite hydrogels displayed not only good antibacterial properties but also good cytocompatibility and histocompatibility.					
34420748	3	49	theme	chemical	584:591	arg1	crosslinking					593:604	chemical crosslinking	584:604	chemical crosslinking	584:604	Herein, triamcinolone acetonide (TA)-loaded chitosan/fucoidan (CF) composite hydrogels were prepared via chemical crosslinking.					
34420748	6	50	theme	fibers	1027:1032	arg1	formation					979:987	the formation	975:987	the formation of mature and well-organized collagen fibers	975:1032	Further, the addition of TA in CF hydrogels improved their elastic feature, inhibited inflammatory response, and promoted the formation of mature and well-organized collagen fibers.					
34420748	2	51	theme	body	463:466	arg1	limitations					347:357	some limitations	342:357	some limitations	342:357	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	2	51	theme	body	463:466	arg1	sensation					468:476	foreign body sensation	455:476	foreign body sensation	455:476	However, most of the current oral mucosa patches still have some limitations, particularly instant, poor mechanical strength and conformability, low adhesion to tissue, and foreign body sensation.					
34420748	1	52	from	lesions	188:194	arg1	cavity					208:213	the oral cavity	199:213	the oral cavity	199:213	Oral inflammatory diseases (OIDs) are among the most common lesions in the oral cavity, affecting the quality of human life and even causing oral cancer.					
34420748	4	53	theme	physicochemical	651:665	arg1	properties					667:676	(bio)physicochemical properties	646:676	(bio)physicochemical properties	646:676	The macro/microscopic morphologies and (bio)physicochemical properties of composite hydrogels were investigated.					
34420748	6	54	theme	collagen	1018:1025	arg1	fibers					1027:1032	mature and well-organized collagen fibers	992:1032	mature and well-organized collagen fibers	992:1032	Further, the addition of TA in CF hydrogels improved their elastic feature, inhibited inflammatory response, and promoted the formation of mature and well-organized collagen fibers.					
34420748	0	55	theme	potential	81:89	arg1	application					91:101	its potential application	77:101	its potential application as an oral mucosa patch	77:125	Preparation of triamcinolone acetonide-loaded chitosan/fucoidan hydrogel and its potential application as an oral mucosa patch.					
34420748	6	56	theme	well-organized	1003:1016	arg1	fibers					1027:1032	mature and well-organized collagen fibers	992:1032	mature and well-organized collagen fibers	992:1032	Further, the addition of TA in CF hydrogels improved their elastic feature, inhibited inflammatory response, and promoted the formation of mature and well-organized collagen fibers.					
34420748	4	57	theme	composite	681:689	arg1	hydrogels					691:699	composite hydrogels	681:699	composite hydrogels	681:699	The macro/microscopic morphologies and (bio)physicochemical properties of composite hydrogels were investigated.					
31961140	6	0	theme	spectrometry	1010:1021	arg1	assay					1032:1036	a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay	952:1036	a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities	952:1153	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	6	0	theme	spectrometry	1010:1021	arg1	CUPRA-ZYME					940:949	CUPRA-ZYME	940:949	CUPRA-ZYME	940:949	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	6	1	theme	ionization	994:1003	arg1	ESI-MS					1024:1029	ESI-MS	1024:1029	ESI-MS	1024:1029	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	6	1	theme	ionization	994:1003	arg1	spectrometry					1010:1021	electrospray ionization mass spectrometry	981:1021	a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities	952:1153	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	7	2	theme	receptor	1226:1233	arg1	CUPRA					1242:1246	CUPRA	1242:1246	CUPRA	1242:1246	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	7	2	theme	receptor	1226:1233	arg1	assay					1235:1239	the recently developed competitive universal proxy receptor assay	1175:1239	the recently developed competitive universal proxy receptor assay (CUPRA)	1175:1247	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	2	3	theme	pathophysiological	284:301	arg1	processes					303:311	diverse physiological and pathophysiological processes	258:311	diverse physiological and pathophysiological processes	258:311	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	6	4	theme	quantitative	968:979	arg1	assay					1032:1036	a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay	952:1036	a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities	952:1153	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	6	4	theme	quantitative	968:979	arg1	CUPRA-ZYME					940:949	CUPRA-ZYME	940:949	CUPRA-ZYME	940:949	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	7	5	theme	universal	1210:1218	arg1	CUPRA					1242:1246	CUPRA	1242:1246	CUPRA	1242:1246	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	7	5	theme	universal	1210:1218	arg1	assay					1235:1239	the recently developed competitive universal proxy receptor assay	1175:1239	the recently developed competitive universal proxy receptor assay (CUPRA)	1175:1247	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	2	6	theme	physiological	266:278	arg1	processes					303:311	diverse physiological and pathophysiological processes	258:311	diverse physiological and pathophysiological processes	258:311	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	6	7	theme	versatile	954:962	arg1	assay					1032:1036	a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay	952:1036	a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities	952:1153	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	6	7	theme	versatile	954:962	arg1	CUPRA-ZYME					940:949	CUPRA-ZYME	940:949	CUPRA-ZYME	940:949	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	7	8	theme	developed	1188:1196	arg1	CUPRA					1242:1246	CUPRA	1242:1246	CUPRA	1242:1246	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	7	8	theme	developed	1188:1196	arg1	assay					1235:1239	the recently developed competitive universal proxy receptor assay	1175:1239	the recently developed competitive universal proxy receptor assay (CUPRA)	1175:1247	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	2	9	theme	important	319:327	arg1	applications					361:372	important industrial and biotechnological applications	319:372	important industrial and biotechnological applications	319:372	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	9	10	theme	substrates	1604:1613	arg1	libraries					1591:1599	libraries	1591:1599	libraries of substrates	1591:1613	Applications to libraries of substrates demonstrate the potential of the assay for quantitative profiling of the substrate specificities glycosidases and glycosyltransferases.					
31961140	6	11	theme	kinetic	1056:1062	arg1	parameters					1064:1073	the kinetic parameters	1052:1073	the kinetic parameters of CAZymes	1052:1084	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	3	12	theme	comprehensive	582:594	arg1	understanding					596:608	a comprehensive understanding	580:608	a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications	580:719	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	8	13	theme	kinetics	1320:1327	arg1	Measurements					1289:1300	Measurements	1289:1300	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3	1289:1435	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	8	14	theme	hydrolase	1405:1413	arg1	neuraminidase					1421:1433	the glycosyl hydrolase human neuraminidase 3	1392:1435	the glycosyl hydrolase human neuraminidase 3	1392:1435	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	10	15	theme	reactivity	1794:1803	arg1	comparison					1776:1785	the comparison	1772:1785	the comparison	1772:1785	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	10	16	theme	natural	1996:2002	arg1	substrates					2019:2028	natural glycoconjugate substrates	1996:2028	natural glycoconjugate substrates	1996:2028	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	8	17	theme	CUPRA	1332:1336	arg1	substrates					1338:1347	CUPRA substrates	1332:1347	CUPRA substrates containing ganglioside oligosaccharides	1332:1387	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	4	18	theme	sensitive	759:767	arg1	techniques					805:814	sensitive and quantitative spectrophotometric techniques	759:814	sensitive and quantitative spectrophotometric techniques	759:814	For glycosyl hydrolases a variety of sensitive and quantitative spectrophotometric techniques are available.					
31961140	3	19	theme	biological	617:626	arg1	functions					628:636	the biological functions	613:636	the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications	613:719	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	9	20	theme	assay	1648:1652	arg1	potential					1631:1639	the potential	1627:1639	the potential of the assay for quantitative profiling of the substrate specificities glycosidases and glycosyltransferases	1627:1748	Applications to libraries of substrates demonstrate the potential of the assay for quantitative profiling of the substrate specificities glycosidases and glycosyltransferases.					
31961140	9	21	dep	specificities	1698:1710	arg1	glycosidases					1712:1723	glycosidases	1712:1723	glycosidases	1712:1723	Applications to libraries of substrates demonstrate the potential of the assay for quantitative profiling of the substrate specificities glycosidases and glycosyltransferases.					
31961140	10	22	theme	CAZyme	2041:2046	arg1	activity					2048:2055	CAZyme activity	2041:2055	CAZyme activity	2041:2055	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	9	23	theme	quantitative	1658:1669	arg1	profiling					1671:1679	quantitative profiling	1658:1679	quantitative profiling of the substrate specificities glycosidases and glycosyltransferases	1658:1748	Applications to libraries of substrates demonstrate the potential of the assay for quantitative profiling of the substrate specificities glycosidases and glycosyltransferases.					
31961140	4	24	theme	quantitative	773:784	arg1	techniques					805:814	sensitive and quantitative spectrophotometric techniques	759:814	sensitive and quantitative spectrophotometric techniques	759:814	For glycosyl hydrolases a variety of sensitive and quantitative spectrophotometric techniques are available.					
31961140	10	25	theme	substrates	2019:2028	arg1	structure					1959:1967	structure	1959:1967	structure	1959:1967	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	10	25	theme	substrates	2019:2028	arg1	environment					1981:1991	protein environment	1973:1991	protein environment	1973:1991	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	6	26	theme	reaction	1098:1105	arg1	pathways					1107:1114	reaction pathways	1098:1114	reaction pathways	1098:1114	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	9	27	theme	substrate	1688:1696	arg1	specificities					1698:1710	the substrate specificities glycosidases and glycosyltransferases	1684:1748	specificities	1698:1710	Applications to libraries of substrates demonstrate the potential of the assay for quantitative profiling of the substrate specificities glycosidases and glycosyltransferases.					
31961140	8	28	theme	kinetic	1475:1481	arg1	parameters					1483:1492	kinetic parameters	1475:1492	kinetic parameters measured by CUPRA-ZYME	1475:1515	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	2	29	theme	promising	417:425	arg1	biomarkers					427:436	promising biomarkers	417:436	promising biomarkers for the diagnosis of a variety of diseases	417:479	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	9	30	theme	glycosyltransferases	1729:1748	arg1	profiling					1671:1679	quantitative profiling	1658:1679	quantitative profiling of the substrate specificities glycosidases and glycosyltransferases	1658:1748	Applications to libraries of substrates demonstrate the potential of the assay for quantitative profiling of the substrate specificities glycosidases and glycosyltransferases.					
31961140	4	31	theme	techniques	805:814	arg1	variety					748:754	a variety	746:754	a variety of sensitive and quantitative spectrophotometric techniques	746:814	For glycosyl hydrolases a variety of sensitive and quantitative spectrophotometric techniques are available.					
31961140	4	31	theme	techniques	805:814	arg1	techniques					805:814	sensitive and quantitative spectrophotometric techniques	759:814	sensitive and quantitative spectrophotometric techniques	759:814	For glycosyl hydrolases a variety of sensitive and quantitative spectrophotometric techniques are available.					
31961140	4	31	theme	techniques	805:814	arg1	available					820:828	available	820:828	available	820:828	For glycosyl hydrolases a variety of sensitive and quantitative spectrophotometric techniques are available.					
31961140	10	32	theme	substrates	1814:1823	arg1	reactivity					1794:1803	the reactivity	1790:1803	the reactivity of CUPRA substrates and glycan substrates present on glycoproteins	1790:1870	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	2	33	theme	drug	389:392	arg1	targets					394:400	important drug targets	379:400	important drug targets	379:400	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	10	34	theme	present	1847:1853	arg1	substrates					1814:1823	CUPRA substrates	1808:1823	CUPRA substrates	1808:1823	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	5	35	theme	glycosyltransferases	866:885	arg1	activity					854:861	the activity	850:861	the activity of glycosyltransferases	850:885	However, measuring the activity of glycosyltransferases is considerably more challenging.					
31961140	2	36	theme	critical	240:247	arg1	roles					249:253	critical roles	240:253	critical roles	240:253	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	2	37	theme	biotechnological	344:359	arg1	applications					361:372	important industrial and biotechnological applications	319:372	important industrial and biotechnological applications	319:372	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	1	38	theme	carbohydrates	215:227	arg1	degradation					182:192	degradation	182:192	degradation	182:192	Carbohydrate-Active enZymes (CAZymes) are involved in the synthesis, degradation, and modification of carbohydrates.					
31961140	1	38	theme	carbohydrates	215:227	arg1	modification					199:210	modification	199:210	modification	199:210	Carbohydrate-Active enZymes (CAZymes) are involved in the synthesis, degradation, and modification of carbohydrates.					
31961140	1	38	theme	carbohydrates	215:227	arg1	synthesis					171:179	synthesis	171:179	synthesis	171:179	Carbohydrate-Active enZymes (CAZymes) are involved in the synthesis, degradation, and modification of carbohydrates.					
31961140	0	39	dep	CUPRA-ZYME	0:9	arg1	Assay					15:19	An Assay	12:19	CUPRA-ZYME: An Assay for Measuring Carbohydrate-Active Enzyme Activities, Pathways, and Substrate Specificities.	0:111	CUPRA-ZYME: An Assay for Measuring Carbohydrate-Active Enzyme Activities, Pathways, and Substrate Specificities.					
31961140	2	40	theme	important	379:387	arg1	targets					394:400	important drug targets	379:400	important drug targets	379:400	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	6	41	theme	mass	1005:1008	arg1	ESI-MS					1024:1029	ESI-MS	1024:1029	ESI-MS	1024:1029	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	6	41	theme	mass	1005:1008	arg1	spectrometry					1010:1021	electrospray ionization mass spectrometry	981:1021	a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities	952:1153	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	7	42	theme	proxy	1220:1224	arg1	CUPRA					1242:1246	CUPRA	1242:1246	CUPRA	1242:1246	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	7	42	theme	proxy	1220:1224	arg1	assay					1235:1239	the recently developed competitive universal proxy receptor assay	1175:1239	the recently developed competitive universal proxy receptor assay (CUPRA)	1175:1247	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	2	43	theme	industrial	329:338	arg1	applications					361:372	important industrial and biotechnological applications	319:372	important industrial and biotechnological applications	319:372	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	6	44	theme	electrospray	981:992	arg1	ESI-MS					1024:1029	ESI-MS	1024:1029	ESI-MS	1024:1029	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	6	44	theme	electrospray	981:992	arg1	spectrometry					1010:1021	electrospray ionization mass spectrometry	981:1021	a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities	952:1153	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	7	45	theme	competitive	1198:1208	arg1	CUPRA					1242:1246	CUPRA	1242:1246	CUPRA	1242:1246	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	7	45	theme	competitive	1198:1208	arg1	assay					1235:1239	the recently developed competitive universal proxy receptor assay	1175:1239	the recently developed competitive universal proxy receptor assay (CUPRA)	1175:1247	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	3	46	theme	specificities	549:561	arg1	Measurements					482:493	Measurements	482:493	Measurements of their activities, catalytic pathway, and substrate specificities	482:561	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	0	47	theme	Carbohydrate-Active	35:53	arg1	Activities					62:71	Carbohydrate-Active Enzyme Activities	35:71	Carbohydrate-Active Enzyme Activities	35:71	CUPRA-ZYME: An Assay for Measuring Carbohydrate-Active Enzyme Activities, Pathways, and Substrate Specificities.					
31961140	10	48	dep	structure	1959:1967	arg1	the					1955:1957	the	1955:1957	the	1955:1957	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	8	49	theme	substrates	1338:1347	arg1	kinetics					1320:1327	the hydrolysis kinetics	1305:1327	the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides	1305:1387	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	10	50	theme	protein	1973:1979	arg1	environment					1981:1991	protein environment	1973:1991	protein environment	1973:1991	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	8	51	contain	containing	1349:1358	arg1	substrates					1338:1347	CUPRA substrates	1332:1347	CUPRA substrates containing ganglioside oligosaccharides	1332:1387	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	8	51	contain	containing	1349:1358	arg2	oligosaccharides					1372:1387	ganglioside oligosaccharides	1360:1387	ganglioside oligosaccharides	1360:1387	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	8	52	theme	ganglioside	1360:1370	arg1	oligosaccharides					1372:1387	ganglioside oligosaccharides	1360:1387	ganglioside oligosaccharides	1360:1387	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	3	53	dep	CAZymes	641:647	arg1	exploiting					653:662	exploiting	653:662	exploiting these enzymes for industrial and biomedical applications	653:719	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	3	54	theme	pathway	526:532	arg1	Measurements					482:493	Measurements	482:493	Measurements of their activities, catalytic pathway, and substrate specificities	482:561	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	10	55	gly	glycoproteins	1858:1870	arg1	glycoproteins					1858:1870	glycoproteins	1858:1870	glycoproteins	1858:1870	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	10	56	theme	glycoconjugate	2004:2017	arg1	substrates					2019:2028	natural glycoconjugate substrates	1996:2028	natural glycoconjugate substrates	1996:2028	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	8	57	theme	hydrolysis	1309:1318	arg1	kinetics					1320:1327	the hydrolysis kinetics	1305:1327	the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides	1305:1387	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	8	58	theme	glycosyl	1396:1403	arg1	neuraminidase					1421:1433	the glycosyl hydrolase human neuraminidase 3	1392:1435	the glycosyl hydrolase human neuraminidase 3	1392:1435	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	1	59	theme	Carbohydrate-Active	113:131	arg1	CAZymes					142:148	CAZymes	142:148	CAZymes	142:148	Carbohydrate-Active enZymes (CAZymes) are involved in the synthesis, degradation, and modification of carbohydrates.					
31961140	1	59	theme	Carbohydrate-Active	113:131	arg1	enZymes					133:139	Carbohydrate-Active enZymes	113:139	Carbohydrate-Active enZymes (CAZymes)	113:149	Carbohydrate-Active enZymes (CAZymes) are involved in the synthesis, degradation, and modification of carbohydrates.					
31961140	8	60	theme	human	1415:1419	arg1	neuraminidase					1421:1433	the glycosyl hydrolase human neuraminidase 3	1392:1435	the glycosyl hydrolase human neuraminidase 3	1392:1435	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	2	61	theme	diverse	258:264	arg1	processes					303:311	diverse physiological and pathophysiological processes	258:311	diverse physiological and pathophysiological processes	258:311	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	3	62	theme	CAZymes	641:647	arg1	functions					628:636	the biological functions	613:636	the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications	613:719	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	4	63	theme	glycosyl	726:733	arg1	hydrolases					735:744	glycosyl hydrolases	726:744	glycosyl hydrolases	726:744	For glycosyl hydrolases a variety of sensitive and quantitative spectrophotometric techniques are available.					
31961140	0	64	theme	Substrate	88:96	arg1	Specificities					98:110	Substrate Specificities	88:110	Substrate Specificities	88:110	CUPRA-ZYME: An Assay for Measuring Carbohydrate-Active Enzyme Activities, Pathways, and Substrate Specificities.					
31961140	9	65	theme	specificities	1698:1710	arg1	profiling					1671:1679	quantitative profiling	1658:1679	quantitative profiling of the substrate specificities glycosidases and glycosyltransferases	1658:1748	Applications to libraries of substrates demonstrate the potential of the assay for quantitative profiling of the substrate specificities glycosidases and glycosyltransferases.					
31961140	6	66	theme	CAZymes	1078:1084	arg1	parameters					1064:1073	the kinetic parameters	1052:1073	the kinetic parameters of CAZymes	1052:1084	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	10	67	attach	present	1847:1853	arg2	substrates					1814:1823	CUPRA substrates	1808:1823	CUPRA substrates	1808:1823	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	10	67	attach	present	1847:1853	arg1	glycoproteins					1858:1870	glycoproteins	1858:1870	glycoproteins	1858:1870	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	3	68	theme	activities	504:513	arg1	Measurements					482:493	Measurements	482:493	Measurements of their activities, catalytic pathway, and substrate specificities	482:561	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	8	69	theme	parameters	1483:1492	arg1	reliability					1460:1470	the reliability	1456:1470	the reliability of kinetic parameters measured by CUPRA-ZYME	1456:1515	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	7	70	theme	time-resolved	1267:1279	arg1	manner					1281:1286	a time-resolved manner	1265:1286	a time-resolved manner	1265:1286	The method employs the recently developed competitive universal proxy receptor assay (CUPRA), implemented in a time-resolved manner.					
31961140	2	71	theme	variety	461:467	arg1	diagnosis					446:454	the diagnosis	442:454	the diagnosis of a variety of diseases	442:479	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	4	72	theme	spectrophotometric	786:803	arg1	techniques					805:814	sensitive and quantitative spectrophotometric techniques	759:814	sensitive and quantitative spectrophotometric techniques	759:814	For glycosyl hydrolases a variety of sensitive and quantitative spectrophotometric techniques are available.					
31961140	10	73	theme	CUPRA	1808:1812	arg1	substrates					1814:1823	CUPRA substrates	1808:1823	CUPRA substrates	1808:1823	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	3	74	theme	functions	628:636	arg1	understanding					596:608	a comprehensive understanding	580:608	a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications	580:719	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	3	75	theme	industrial	682:691	arg1	applications					708:719	industrial and biomedical applications	682:719	industrial and biomedical applications	682:719	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	6	76	theme	substrate	1131:1139	arg1	specificities					1141:1153	substrate specificities	1131:1153	substrate specificities	1131:1153	Here, we introduce CUPRA-ZYME, a versatile and quantitative electrospray ionization mass spectrometry (ESI-MS) assay for measuring the kinetic parameters of CAZymes, monitoring reaction pathways, and profiling substrate specificities.					
31961140	10	77	theme	glycan	1829:1834	arg1	substrates					1836:1845	glycan substrates	1829:1845	glycan substrates	1829:1845	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	10	78	theme	substrates	1836:1845	arg1	reactivity					1794:1803	the reactivity	1790:1803	the reactivity of CUPRA substrates and glycan substrates present on glycoproteins	1790:1870	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	0	79	theme	Enzyme	55:60	arg1	Activities					62:71	Carbohydrate-Active Enzyme Activities	35:71	Carbohydrate-Active Enzyme Activities	35:71	CUPRA-ZYME: An Assay for Measuring Carbohydrate-Active Enzyme Activities, Pathways, and Substrate Specificities.					
31961140	2	80	contain	have	314:317	arg1	They					230:233	They	230:233	They	230:233	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	2	80	contain	have	314:317	arg2	applications					361:372	important industrial and biotechnological applications	319:372	important industrial and biotechnological applications	319:372	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	2	81	theme	diseases	472:479	arg1	variety					461:467	a variety	459:467	a variety of diseases	459:479	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	2	81	theme	diseases	472:479	arg1	diseases					472:479	diseases	472:479	diseases	472:479	They play critical roles in diverse physiological and pathophysiological processes, have important industrial and biotechnological applications, are important drug targets, and represent promising biomarkers for the diagnosis of a variety of diseases.					
31961140	1	82	dep	synthesis	171:179	arg1	the					167:169	the	167:169	the	167:169	Carbohydrate-Active enZymes (CAZymes) are involved in the synthesis, degradation, and modification of carbohydrates.					
31961140	3	83	theme	catalytic	516:524	arg1	pathway					526:532	catalytic pathway	516:532	catalytic pathway	516:532	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	3	84	theme	biomedical	697:706	arg1	applications					708:719	industrial and biomedical applications	682:719	industrial and biomedical applications	682:719	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
31961140	8	85	theme	catalytic	1555:1563	arg1	pathways					1565:1572	catalytic pathways	1555:1572	catalytic pathways	1555:1572	Measurements of the hydrolysis kinetics of CUPRA substrates containing ganglioside oligosaccharides by the glycosyl hydrolase human neuraminidase 3 served to validate the reliability of kinetic parameters measured by CUPRA-ZYME and highlight its use in establishing catalytic pathways.					
31961140	10	86	theme	unique	1908:1913	arg1	opportunity					1915:1925	a unique opportunity	1906:1925	a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity	1906:2055	Finally, we show how the comparison of the reactivity of CUPRA substrates and glycan substrates present on glycoproteins, measured simultaneously, affords a unique opportunity to quantitatively study how the structure and protein environment of natural glycoconjugate substrates influences CAZyme activity.					
31961140	3	87	theme	substrate	539:547	arg1	specificities					549:561	substrate specificities	539:561	substrate specificities	539:561	Measurements of their activities, catalytic pathway, and substrate specificities are essential to a comprehensive understanding of the biological functions of CAZymes and exploiting these enzymes for industrial and biomedical applications.					
33522177	4	0	from	problems	503:510	arg1	research					519:526	our research	515:526	our research	515:526	It was also pointed out that the existing problems in our research, which need to be solved urgently.					
33522177	1	1	theme	implantable	150:160	arg1	devices					170:176	implantable medical devices	150:176	implantable medical devices	150:176	It is well known that chitosan-based composites are widely used in implantable medical devices.					
33522177	0	2	from	[Advances	0:8	arg1	Research					13:20	Research	13:20	Research of Chitosan-based Composites for Implanted Medical Devices	13:79	[Advances in Research of Chitosan-based Composites for Implanted Medical Devices].					
33522177	5	3	theme	chitosan-based	632:645	arg1	composites					647:656	chitosan-based composites	632:656	chitosan-based composites	632:656	At last, the development direction and broad application prospect of chitosan-based composites were prospected.					
33522177	5	4	theme	composites	647:656	arg1	application					608:618	broad application	602:618	broad application	602:618	At last, the development direction and broad application prospect of chitosan-based composites were prospected.					
33522177	5	4	theme	composites	647:656	arg1	development					576:586	the development direction and broad application prospect	572:627	development	576:586	At last, the development direction and broad application prospect of chitosan-based composites were prospected.					
33522177	1	5	used	used	142:145	arg2	composites					120:129	chitosan-based composites	105:129	chitosan-based composites	105:129	It is well known that chitosan-based composites are widely used in implantable medical devices.					
33522177	1	6	theme	medical	162:168	arg1	devices					170:176	implantable medical devices	150:176	implantable medical devices	150:176	It is well known that chitosan-based composites are widely used in implantable medical devices.					
33522177	3	7	theme	3D	392:393	arg1	technology					404:413	3D printing technology	392:413	3D printing technology	392:413	Then, combined with the research focus, the development of 3D printing technology and chitosan-based composites was summarized.					
33522177	3	8	theme	composites	434:443	arg1	development					377:387	the development	373:387	the development of 3D printing technology and chitosan-based composites	373:443	Then, combined with the research focus, the development of 3D printing technology and chitosan-based composites was summarized.					
33522177	5	9	theme	broad	602:606	arg1	application					608:618	broad application	602:618	broad application	602:618	At last, the development direction and broad application prospect of chitosan-based composites were prospected.					
33522177	6	10	dep	we	679:680	arg1	look					682:685	look	682:685	we look	679:685	And we look forward to providing reference for relevant research.					
33522177	3	11	theme	technology	404:413	arg1	development					377:387	the development	373:387	the development of 3D printing technology and chitosan-based composites	373:443	Then, combined with the research focus, the development of 3D printing technology and chitosan-based composites was summarized.					
33522177	2	12	theme	organic	303:309	arg1	phases					325:330	inorganic, organic and composite phases	292:330	inorganic, organic and composite phases	292:330	The development of chitosan-based composite materials with different types was summarized in this paper, such as inorganic, organic and composite phases.					
33522177	3	13	theme	research	357:364	arg1	focus					366:370	the research focus	353:370	the research focus	353:370	Then, combined with the research focus, the development of 3D printing technology and chitosan-based composites was summarized.					
33522177	2	14	with	materials	223:231	arg1	types					248:252	different types	238:252	different types	238:252	The development of chitosan-based composite materials with different types was summarized in this paper, such as inorganic, organic and composite phases.					
33522177	2	15	theme	inorganic	292:300	arg1	phases					325:330	inorganic, organic and composite phases	292:330	inorganic, organic and composite phases	292:330	The development of chitosan-based composite materials with different types was summarized in this paper, such as inorganic, organic and composite phases.					
33522177	0	16	theme	Composites	40:49	arg1	Research					13:20	Research	13:20	Research of Chitosan-based Composites for Implanted Medical Devices	13:79	[Advances in Research of Chitosan-based Composites for Implanted Medical Devices].					
33522177	0	17	theme	Chitosan-based	25:38	arg1	Composites					40:49	Chitosan-based Composites	25:49	Chitosan-based Composites	25:49	[Advances in Research of Chitosan-based Composites for Implanted Medical Devices].					
33522177	3	18	theme	printing	395:402	arg1	technology					404:413	3D printing technology	392:413	3D printing technology	392:413	Then, combined with the research focus, the development of 3D printing technology and chitosan-based composites was summarized.					
33522177	2	19	theme	different	238:246	arg1	types					248:252	different types	238:252	different types	238:252	The development of chitosan-based composite materials with different types was summarized in this paper, such as inorganic, organic and composite phases.					
33522177	0	20	theme	Implanted	55:63	arg1	Devices					73:79	Implanted Medical Devices	55:79	Implanted Medical Devices	55:79	[Advances in Research of Chitosan-based Composites for Implanted Medical Devices].					
33522177	3	21	theme	chitosan-based	419:432	arg1	composites					434:443	chitosan-based composites	419:443	chitosan-based composites	419:443	Then, combined with the research focus, the development of 3D printing technology and chitosan-based composites was summarized.					
33522177	4	22	theme	existing	494:501	arg1	problems					503:510	the existing problems	490:510	the existing problems	490:510	It was also pointed out that the existing problems in our research, which need to be solved urgently.					
33522177	2	23	theme	composite	213:221	arg1	materials					223:231	chitosan-based composite materials	198:231	chitosan-based composite materials with different types	198:252	The development of chitosan-based composite materials with different types was summarized in this paper, such as inorganic, organic and composite phases.					
33522177	2	24	theme	materials	223:231	arg1	development					183:193	The development	179:193	The development of chitosan-based composite materials with different types	179:252	The development of chitosan-based composite materials with different types was summarized in this paper, such as inorganic, organic and composite phases.					
33522177	1	25	theme	chitosan-based	105:118	arg1	composites					120:129	chitosan-based composites	105:129	chitosan-based composites	105:129	It is well known that chitosan-based composites are widely used in implantable medical devices.					
33522177	2	26	theme	chitosan-based	198:211	arg1	materials					223:231	chitosan-based composite materials	198:231	chitosan-based composite materials with different types	198:252	The development of chitosan-based composite materials with different types was summarized in this paper, such as inorganic, organic and composite phases.					
33522177	5	27	dep	development	576:586	arg1	direction					588:596	direction	588:596	direction	588:596	At last, the development direction and broad application prospect of chitosan-based composites were prospected.					
33522177	5	27	dep	development	576:586	arg1	prospect					620:627	prospect	620:627	prospect	620:627	At last, the development direction and broad application prospect of chitosan-based composites were prospected.					
33522177	6	28	theme	relevant	722:729	arg1	research					731:738	relevant research	722:738	relevant research	722:738	And we look forward to providing reference for relevant research.					
33522177	2	29	theme	composite	315:323	arg1	phases					325:330	inorganic, organic and composite phases	292:330	inorganic, organic and composite phases	292:330	The development of chitosan-based composite materials with different types was summarized in this paper, such as inorganic, organic and composite phases.					
33522177	0	30	theme	Medical	65:71	arg1	Devices					73:79	Implanted Medical Devices	55:79	Implanted Medical Devices	55:79	[Advances in Research of Chitosan-based Composites for Implanted Medical Devices].					
34342521	5	0	theme	ultrasound	1125:1134	arg1	field					1118:1122	pulsed electric field	1102:1122	pulsed electric field	1102:1122	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	0	theme	ultrasound	1125:1134	arg1	treatment					1136:1144	ultrasound treatment	1125:1144	ultrasound treatment	1125:1144	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	3	1	theme	grape	493:497	arg1	polysaccharides					499:513	grape polysaccharides	493:513	grape polysaccharides	493:513	First, the composition of grape polysaccharides and their changes during grape ripening, winemaking and aging are introduced.					
34342521	0	2	theme	possible	66:73	arg1	effects					75:81	possible effects	66:81	possible effects on wine organoleptic properties	66:113	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	3	3	theme	polysaccharides	499:513	arg1	composition					478:488	the composition	474:488	the composition of grape polysaccharides	474:513	First, the composition of grape polysaccharides and their changes during grape ripening, winemaking and aging are introduced.					
34342521	3	3	theme	polysaccharides	499:513	arg1	changes					525:531	their changes	519:531	their changes during grape ripening, winemaking and aging	519:575	First, the composition of grape polysaccharides and their changes during grape ripening, winemaking and aging are introduced.					
34342521	5	4	theme	vinification	1075:1086	arg1	technologies					1088:1099	modern vinification technologies	1068:1099	modern vinification technologies (pulsed electric field, ultrasound treatment)	1068:1145	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	0	5	theme	wine	86:89	arg1	properties					104:113	wine organoleptic properties	86:113	wine organoleptic properties	86:113	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	4	6	dep	compounds	683:691	arg1	anthocyanins					705:716	anthocyanins	705:716	anthocyanins	705:716	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	4	6	dep	compounds	683:691	arg1	flavanols					694:702	flavanols	694:702	flavanols	694:702	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	4	6	dep	compounds	683:691	arg1	volatiles					722:730	volatiles	722:730	volatiles	722:730	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	5	7	from	content	906:912	arg1	practice					917:924	practice	917:924	practice	917:924	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	3	8	theme	grape	540:544	arg1	ripening					546:553	grape ripening	540:553	grape ripening	540:553	First, the composition of grape polysaccharides and their changes during grape ripening, winemaking and aging are introduced.					
34342521	5	9	theme	winemaking	960:969	arg1	methods					971:977	classical winemaking methods	950:977	classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending)	950:1065	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	10	from	control	870:876	arg1	practice					917:924	practice	917:924	practice	917:924	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	11	theme	electric	1109:1116	arg1	field					1118:1122	pulsed electric field	1102:1122	pulsed electric field	1102:1122	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	11	theme	electric	1109:1116	arg1	treatment					1136:1144	ultrasound treatment	1125:1144	ultrasound treatment	1125:1144	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	12	theme	polysaccharide	891:904	arg1	content					906:912	the grape polysaccharide content	881:912	the grape polysaccharide content in practice	881:924	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	1	13	theme	different	276:284	arg1	mechanisms					286:295	different mechanisms	276:295	different mechanisms	276:295	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	4	14	from	effects	766:772	arg1	aroma					805:809	aroma	805:809	aroma	805:809	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	4	14	from	effects	766:772	arg1	color					795:799	color	795:799	color	795:799	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	4	14	from	effects	766:772	arg1	astringency					782:792	wine astringency	777:792	wine astringency	777:792	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	1	15	theme	present	173:179	arg1	Polysaccharides					157:171	Polysaccharides	157:171	Polysaccharides present in grapes	157:189	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	0	16	theme	organoleptic	91:102	arg1	properties					104:113	wine organoleptic properties	86:113	wine organoleptic properties	86:113	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	4	17	theme	wine	777:780	arg1	astringency					782:792	wine astringency	777:792	wine astringency	777:792	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	4	18	theme	wine	663:666	arg1	compounds					683:691	wine sensory-active compounds	663:691	wine sensory-active compounds (flavanols, anthocyanins and volatiles)	663:731	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	0	19	from	changes	37:43	arg1	wines					59:63	wines	59:63	wines	59:63	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	0	19	from	changes	37:43	arg1	grapes					48:53	grapes	48:53	grapes	48:53	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	0	19	from	changes	37:43	arg1	properties					104:113	wine organoleptic properties	86:113	wine organoleptic properties	86:113	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	5	20	theme	new	1171:1173	arg1	products					1196:1203	new grape polysaccharide products	1171:1203	new grape polysaccharide products	1171:1203	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	0	21	theme	Grape	0:4	arg1	polysaccharides					6:20	Grape polysaccharides	0:20	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.	0:155	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	5	22	theme	grape	1175:1179	arg1	products					1196:1203	new grape polysaccharide products	1171:1203	new grape polysaccharide products	1171:1203	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	23	theme	modern	1068:1073	arg1	technologies					1088:1099	modern vinification technologies	1068:1099	modern vinification technologies (pulsed electric field, ultrasound treatment)	1068:1145	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	2	24	from	Studies	378:384	arg1	role					393:396	the role	389:396	the role that grape polysaccharides play in wines	389:437	Studies on the role that grape polysaccharides play in wines are reviewed in this paper.					
34342521	4	25	theme	compounds	683:691	arg1	mechanisms					623:632	different interaction mechanisms	601:632	different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles)	601:731	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	0	26	theme	compositional	23:35	arg1	changes					37:43	compositional changes	23:43	compositional changes in grapes and wines	23:63	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	5	27	theme	polysaccharide	1181:1194	arg1	products					1196:1203	new grape polysaccharide products	1171:1203	new grape polysaccharide products	1171:1203	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	28	from	practice	917:924	arg1	control					870:876	the control	866:876	the control of the grape polysaccharide content in practice	866:924	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	4	29	theme	possible	757:764	arg1	effects					766:772	the possible effects	753:772	the possible effects on wine astringency, color and aroma caused by these interactions	753:838	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	4	30	theme	grape	637:641	arg1	polysaccharides					643:657	grape polysaccharides	637:657	grape polysaccharides	637:657	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	5	31	theme	products	1196:1203	arg1	technologies					1088:1099	modern vinification technologies	1068:1099	modern vinification technologies (pulsed electric field, ultrasound treatment)	1068:1145	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	31	theme	products	1196:1203	arg1	development					1156:1166	the development	1152:1166	the development of new grape polysaccharide products	1152:1203	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	31	theme	products	1196:1203	arg1	methods					971:977	classical winemaking methods	950:977	classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending)	950:1065	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	0	32	from	effects	75:81	arg1	wines					59:63	wines	59:63	wines	59:63	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	0	32	from	effects	75:81	arg1	grapes					48:53	grapes	48:53	grapes	48:53	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	0	32	from	effects	75:81	arg1	properties					104:113	wine organoleptic properties	86:113	wine organoleptic properties	86:113	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	5	33	theme	content	906:912	arg1	control					870:876	the control	866:876	the control of the grape polysaccharide content in practice	866:924	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	1	34	theme	wine	205:208	arg1	compounds					225:233	wine sensory-active compounds	205:233	wine sensory-active compounds (polyphenols and volatile compounds)	205:270	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	35	attach	present	173:179	arg2	Polysaccharides					157:171	Polysaccharides	157:171	Polysaccharides present in grapes	157:189	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	35	attach	present	173:179	arg1	grapes					184:189	grapes	184:189	grapes	184:189	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	36	theme	sensory-active	210:223	arg1	compounds					225:233	wine sensory-active compounds	205:233	wine sensory-active compounds (polyphenols and volatile compounds)	205:270	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	4	37	theme	sensory-active	668:681	arg1	compounds					683:691	wine sensory-active compounds	663:691	wine sensory-active compounds (flavanols, anthocyanins and volatiles)	663:731	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	1	38	theme	wine	312:315	arg1	aroma					371:375	aroma	371:375	aroma	371:375	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	38	theme	wine	312:315	arg1	color					361:365	color	361:365	color	361:365	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	38	theme	wine	312:315	arg1	qualities					330:338	wine organoleptic qualities	312:338	wine organoleptic qualities such as astringency, color and aroma	312:375	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	38	theme	wine	312:315	arg1	astringency					348:358	astringency	348:358	astringency	348:358	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	0	39	theme	practical	120:128	arg1	control					130:136	practical control	120:136	practical control during winemaking	120:154	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	2	40	theme	grape	403:407	arg1	polysaccharides					409:423	grape polysaccharides	403:423	grape polysaccharides	403:423	Studies on the role that grape polysaccharides play in wines are reviewed in this paper.					
34342521	1	41	theme	organoleptic	317:328	arg1	aroma					371:375	aroma	371:375	aroma	371:375	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	41	theme	organoleptic	317:328	arg1	color					361:365	color	361:365	color	361:365	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	41	theme	organoleptic	317:328	arg1	qualities					330:338	wine organoleptic qualities	312:338	wine organoleptic qualities such as astringency, color and aroma	312:375	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	41	theme	organoleptic	317:328	arg1	astringency					348:358	astringency	348:358	astringency	348:358	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	5	42	theme	pulsed	1102:1107	arg1	field					1118:1122	pulsed electric field	1102:1122	pulsed electric field	1102:1122	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	42	theme	pulsed	1102:1107	arg1	treatment					1136:1144	ultrasound treatment	1125:1144	ultrasound treatment	1125:1144	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	43	theme	temperature	1019:1029	arg1	blending					1057:1064	blending	1057:1064	blending	1057:1064	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	43	theme	temperature	1019:1029	arg1	co-fermentation					1040:1054	co-fermentation	1040:1054	co-fermentation	1040:1054	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	43	theme	temperature	1019:1029	arg1	enzymes					1010:1016	different maceration enzymes	989:1016	different maceration enzymes	989:1016	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	43	theme	temperature	1019:1029	arg1	control					1031:1037	temperature control	1019:1037	temperature control	1019:1037	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	44	theme	maceration	999:1008	arg1	enzymes					1010:1016	different maceration enzymes	989:1016	different maceration enzymes	989:1016	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	44	theme	maceration	999:1008	arg1	control					1031:1037	temperature control	1019:1037	temperature control	1019:1037	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	45	dep	methods	971:977	arg1	applying					980:987	applying	980:987	applying different maceration enzymes, temperature control, co-fermentation, blending	980:1064	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	46	theme	classical	950:958	arg1	methods					971:977	classical winemaking methods	950:977	classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending)	950:1065	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	0	47	from	control	130:136	arg1	wines					59:63	wines	59:63	wines	59:63	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	0	47	from	control	130:136	arg1	grapes					48:53	grapes	48:53	grapes	48:53	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	0	47	from	control	130:136	arg1	properties					104:113	wine organoleptic properties	86:113	wine organoleptic properties	86:113	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	5	48	theme	different	989:997	arg1	enzymes					1010:1016	different maceration enzymes	989:1016	different maceration enzymes	989:1016	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	48	theme	different	989:997	arg1	control					1031:1037	temperature control	1019:1037	temperature control	1019:1037	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	1	49	dep	compounds	225:233	arg1	polyphenols					236:246	polyphenols	236:246	polyphenols	236:246	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	49	dep	compounds	225:233	arg1	compounds					261:269	volatile compounds	252:269	volatile compounds	252:269	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	1	50	from	grapes	184:189	arg1	present					173:179	present	173:179	present	173:179	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	5	51	theme	grape	885:889	arg1	content					906:912	the grape polysaccharide content	881:912	the grape polysaccharide content in practice	881:924	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	4	52	theme	polysaccharides	643:657	arg1	mechanisms					623:632	different interaction mechanisms	601:632	different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles)	601:731	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	0	53	dep	polysaccharides	6:20	arg1	control					130:136	practical control	120:136	practical control during winemaking	120:154	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	0	53	dep	polysaccharides	6:20	arg1	effects					75:81	possible effects	66:81	possible effects on wine organoleptic properties	66:113	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	0	53	dep	polysaccharides	6:20	arg1	changes					37:43	compositional changes	23:43	compositional changes in grapes and wines	23:63	Grape polysaccharides: compositional changes in grapes and wines, possible effects on wine organoleptic properties, and practical control during winemaking.					
34342521	4	54	theme	interaction	611:621	arg1	mechanisms					623:632	different interaction mechanisms	601:632	different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles)	601:731	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	1	55	from	present	173:179	arg1	grapes					184:189	grapes	184:189	grapes	184:189	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
34342521	4	56	theme	different	601:609	arg1	mechanisms					623:632	different interaction mechanisms	601:632	different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles)	601:731	Second, different interaction mechanisms of grape polysaccharides and wine sensory-active compounds (flavanols, anthocyanins and volatiles) are introduced, and the possible effects on wine astringency, color and aroma caused by these interactions are illustrated.					
34342521	5	57	dep	technologies	1088:1099	arg1	field					1118:1122	pulsed electric field	1102:1122	pulsed electric field	1102:1122	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	5	57	dep	technologies	1088:1099	arg1	treatment					1136:1144	ultrasound treatment	1125:1144	ultrasound treatment	1125:1144	Finally, the control of the grape polysaccharide content in practice is discussed, including classical winemaking methods (applying different maceration enzymes, temperature control, co-fermentation, blending), modern vinification technologies (pulsed electric field, ultrasound treatment), and the development of new grape polysaccharide products.					
34342521	1	58	theme	volatile	252:259	arg1	compounds					261:269	volatile compounds	252:269	volatile compounds	252:269	Polysaccharides present in grapes interact with wine sensory-active compounds (polyphenols and volatile compounds) via different mechanisms and can affect wine organoleptic qualities such as astringency, color and aroma.					
32739918	9	0	theme	CD4+	1613:1616	arg1	Teffs					1618:1622	CD4+ Teffs	1613:1622	CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase	1613:1713	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	7	1	theme	host-related	1075:1086	arg1	levels					1102:1107	host-related PD1 and IL-10 levels	1075:1107	host-related PD1 and IL-10 levels	1075:1107	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	7	1	theme	host-related	1075:1086	arg1	immunoregulators					1136:1151	the key immunoregulators	1128:1151	the key immunoregulators	1128:1151	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	9	2	theme	E.	1867:1868	arg1	metacestode					1885:1895	E. multilocularis metacestode	1867:1895	E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase	1867:1935	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	11	3	theme	enzymes	2220:2226	arg1	administration					2198:2211	therapeutical in vivo administration	2176:2211	therapeutical in vivo administration of the enzymes	2176:2226	These findings justify a continuation of these experiments upon therapeutical in vivo administration of the enzymes.					
32739918	9	4	from	mice	1846:1849	arg1	Teff					1836:1839	CD4+ Teff	1831:1839	CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase	1831:1935	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	7	5	theme	weight	1051:1056	arg1	measurement					1058:1068	wet weight measurement	1047:1068	wet weight measurement	1047:1068	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	3	6	dep	attack	528:533	arg1	or					536:537	or	536:537	or kill	536:542	This should allow the host defense to more easily recognize and attack (or kill) the parasite by immune-mediated effects.					
32739918	3	6	dep	attack	528:533	arg1	parasite					549:556	the parasite	545:556	the parasite by immune-mediated effects	545:583	This should allow the host defense to more easily recognize and attack (or kill) the parasite by immune-mediated effects.					
32739918	9	7	theme	E.	1650:1651	arg1	metacestode					1668:1678	E. multilocularis metacestode	1650:1678	E. multilocularis metacestode pretreated with β1-3-galactosidase	1650:1713	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	4	8	theme	E.	610:611	arg1	metacestodes					640:651	E. multilocularis (clone H95) metacestodes	610:651	E. multilocularis (clone H95) metacestodes	610:651	METHODS Experimentally, E. multilocularis (clone H95) metacestodes were cultured in vitro with or without addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium.					
32739918	4	8	theme	E.	610:611	arg1	H95					635:637	clone H95	629:637	clone H95	629:637	METHODS Experimentally, E. multilocularis (clone H95) metacestodes were cultured in vitro with or without addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium.					
32739918	5	9	theme	time	840:843	arg1	points					845:850	different time points	830:850	different time points	830:850	Morphological changes were subsequently measured by microscopy at different time points.					
32739918	8	10	theme	parasite	1224:1231	arg1	structure					1243:1251	the parasite vesicular structure	1220:1251	the parasite vesicular structure	1220:1251	RESULTS Our experiments demonstrated that the parasite vesicular structure can be directly destroyed by adding galactosidases into the in vitro culture system, resulting in the fact that the parasite metacestode vesicles could not anymore infect and develop in mice after this glycan digestion.					
32739918	9	11	theme	E.	1525:1526	arg1	metacestode					1543:1553	E. multilocularis metacestode	1525:1553	E. multilocularis metacestode without galactosidases	1525:1576	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	1	12	theme	glycosylated	170:181	arg1	INTRODUCTION					101:112	INTRODUCTION	101:112	INTRODUCTION The E. multilocularis laminated layer (LL)	101:155	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	1	12	theme	glycosylated	170:181	arg1	structure					193:201	a heavily glycosylated parasitic structure	160:201	a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions	160:344	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	10	13	theme	"	1993:1993	arg1	effect					1995:2000	this "aborting" effect	1979:2000	this "aborting" effect	1979:2000	DISCUSSION We raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection.					
32739918	9	14	from	mice	1629:1632	arg1	Teffs					1618:1622	CD4+ Teffs	1613:1622	CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase	1613:1713	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	7	15	theme	respective	1007:1016	arg1	load					1018:1021	the respective load	1003:1021	the respective load	1003:1021	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	1	16	theme	E.	118:119	arg1	LL					153:154	LL	153:154	LL	153:154	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	1	16	theme	E.	118:119	arg1	layer					146:150	The E. multilocularis laminated layer	114:150	INTRODUCTION The E. multilocularis laminated layer (LL)	101:155	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	2	17	theme	targeted	444:451	arg1	digestion					453:461	a targeted digestion	442:461	a targeted digestion	442:461	We elaborated an experimental design with the idea to modify the (glycan) surface of the LL by a targeted digestion.					
32739918	11	18	theme	experiments	2159:2169	arg1	continuation					2137:2148	a continuation	2135:2148	a continuation of these experiments	2135:2169	These findings justify a continuation of these experiments upon therapeutical in vivo administration of the enzymes.					
32739918	1	19	theme	laminated	136:144	arg1	LL					153:154	LL	153:154	LL	153:154	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	1	19	theme	laminated	136:144	arg1	layer					146:150	The E. multilocularis laminated layer	114:150	INTRODUCTION The E. multilocularis laminated layer (LL)	101:155	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	0	20	theme	Experimental	43:54	arg1	Concept					56:62	A New Experimental Concept	37:62	the Parasite: A New Experimental Concept	23:62	Digest the Sugar, Kill the Parasite: A New Experimental Concept in Treating Alveolar Echinococcosis.					
32739918	6	21	theme	infectious	951:960	arg1	status					962:967	infectious status	951:967	infectious status	951:967	Parasites were then recovered at day 5 and reinjected into mice for assessing their viability and infectious status.					
32739918	8	22	theme	in	1313:1314	arg1	system					1330:1335	the in vitro culture system	1309:1335	the in vitro culture system	1309:1335	RESULTS Our experiments demonstrated that the parasite vesicular structure can be directly destroyed by adding galactosidases into the in vitro culture system, resulting in the fact that the parasite metacestode vesicles could not anymore infect and develop in mice after this glycan digestion.					
32739918	1	23	theme	host	331:334	arg1	reactions					336:344	physiological and immunological host reactions	299:344	physiological and immunological host reactions	299:344	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	4	24	from	addition	692:699	arg1	medium					756:761	the medium	752:761	the medium	752:761	METHODS Experimentally, E. multilocularis (clone H95) metacestodes were cultured in vitro with or without addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium.					
32739918	11	25	dep	in	2190:2191	arg1	vivo					2193:2196	vivo	2193:2196	vivo	2193:2196	These findings justify a continuation of these experiments upon therapeutical in vivo administration of the enzymes.					
32739918	4	26	theme	α1-3,4,6-galactosidase	704:725	arg1	addition					692:699	addition	692:699	addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium	692:761	METHODS Experimentally, E. multilocularis (clone H95) metacestodes were cultured in vitro with or without addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium.					
32739918	0	27	theme	Alveolar	76:83	arg1	Echinococcosis					85:98	Alveolar Echinococcosis	76:98	Alveolar Echinococcosis	76:98	Digest the Sugar, Kill the Parasite: A New Experimental Concept in Treating Alveolar Echinococcosis.					
32739918	9	28	dep	E.	1650:1651	arg1	multilocularis					1653:1666	multilocularis	1653:1666	multilocularis	1653:1666	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	2	29	theme	glycan	413:418	arg1	surface					421:427	the (glycan) surface	408:427	the (glycan) surface of the LL	408:437	We elaborated an experimental design with the idea to modify the (glycan) surface of the LL by a targeted digestion.					
32739918	9	30	theme	IL-10	1729:1733	arg1	secretion					1735:1743	a lower IL-10 secretion	1721:1743	a lower IL-10 secretion from CD4+ Teffs	1721:1759	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	1	31	theme	larval	250:255	arg1	metacestode					264:274	metacestode	264:274	metacestode	264:274	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	1	31	theme	larval	250:255	arg1	stage					257:261	the larval stage	246:261	the larval stage (metacestode) of this parasite	246:292	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	7	32	theme	wet	1047:1049	arg1	weight					1051:1056	wet weight	1047:1056	wet weight measurement	1047:1068	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	8	33	dep	RESULTS	1178:1184	arg1	demonstrated					1202:1213	demonstrated	1202:1213	demonstrated that the parasite vesicular structure can be directly destroyed by adding galactosidases into the in vitro culture system, resulting in the fact that the parasite metacestode vesicles could not anymore infect and develop in mice after this glycan digestion	1202:1470	RESULTS Our experiments demonstrated that the parasite vesicular structure can be directly destroyed by adding galactosidases into the in vitro culture system, resulting in the fact that the parasite metacestode vesicles could not anymore infect and develop in mice after this glycan digestion.					
32739918	10	34	dep	DISCUSSION	1938:1947	arg1	raised					1952:1957	raised	1952:1957	raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection	1952:2109	DISCUSSION We raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection.					
32739918	9	35	from	Teffs	1755:1759	arg1	secretion					1735:1743	a lower IL-10 secretion	1721:1743	a lower IL-10 secretion from CD4+ Teffs	1721:1759	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	0	36	dep	Parasite	27:34	arg1	Concept					56:62	A New Experimental Concept	37:62	the Parasite: A New Experimental Concept	23:62	Digest the Sugar, Kill the Parasite: A New Experimental Concept in Treating Alveolar Echinococcosis.					
32739918	9	37	theme	expression	1803:1812	arg1	levels					1814:1819	IL-10 expression levels	1797:1819	IL-10 expression levels	1797:1819	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	3	38	theme	immune-mediated	561:575	arg1	effects					577:583	immune-mediated effects	561:583	immune-mediated effects	561:583	This should allow the host defense to more easily recognize and attack (or kill) the parasite by immune-mediated effects.					
32739918	9	39	theme	PD1	1579:1581	arg1	expression					1583:1592	PD1 expression	1579:1592	PD1 expression	1579:1592	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	8	40	theme	metacestode	1378:1388	arg1	vesicles					1390:1397	the parasite metacestode vesicles	1365:1397	the parasite metacestode vesicles	1365:1397	RESULTS Our experiments demonstrated that the parasite vesicular structure can be directly destroyed by adding galactosidases into the in vitro culture system, resulting in the fact that the parasite metacestode vesicles could not anymore infect and develop in mice after this glycan digestion.					
32739918	10	41	theme	IL-10	2038:2042	arg1	fine-tuning					2053:2063	an altered PD1 and IL-10 response fine-tuning	2019:2063	fine-tuning	2053:2063	DISCUSSION We raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection.					
32739918	11	42	theme	in	2190:2191	arg1	administration					2198:2211	therapeutical in vivo administration	2176:2211	therapeutical in vivo administration of the enzymes	2176:2226	These findings justify a continuation of these experiments upon therapeutical in vivo administration of the enzymes.					
32739918	4	43	dep	METHODS	586:592	arg1	cultured					658:665	cultured	658:665	were cultured in vitro with or without addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium	653:761	METHODS Experimentally, E. multilocularis (clone H95) metacestodes were cultured in vitro with or without addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium.					
32739918	10	44	theme	immune	2093:2098	arg1	protection					2100:2109	immune protection	2093:2109	immune protection	2093:2109	DISCUSSION We raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection.					
32739918	2	45	with	design	377:382	arg1	idea					393:396	the idea	389:396	the idea	389:396	We elaborated an experimental design with the idea to modify the (glycan) surface of the LL by a targeted digestion.					
32739918	3	46	theme	host	486:489	arg1	defense					491:497	the host defense	482:497	the host defense	482:497	This should allow the host defense to more easily recognize and attack (or kill) the parasite by immune-mediated effects.					
32739918	1	47	gly	glycosylated	170:181	arg1	INTRODUCTION					101:112	INTRODUCTION	101:112	INTRODUCTION The E. multilocularis laminated layer (LL)	101:155	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	1	47	gly	glycosylated	170:181	arg1	structure					193:201	a heavily glycosylated parasitic structure	160:201	a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions	160:344	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	2	48	theme	LL	436:437	arg1	surface					421:427	the (glycan) surface	408:427	the (glycan) surface of the LL	408:437	We elaborated an experimental design with the idea to modify the (glycan) surface of the LL by a targeted digestion.					
32739918	10	49	attach	linked	2009:2014	arg2	effect					1995:2000	this "aborting" effect	1979:2000	this "aborting" effect	1979:2000	DISCUSSION We raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection.					
32739918	10	49	attach	linked	2009:2014	arg1	fine-tuning					2053:2063	an altered PD1 and IL-10 response fine-tuning	2019:2063	fine-tuning	2053:2063	DISCUSSION We raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection.					
32739918	10	49	attach	linked	2009:2014	arg1	PD1					2030:2032	an altered PD1 and IL-10 response fine-tuning	2019:2063	PD1	2030:2032	DISCUSSION We raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection.					
32739918	5	50	theme	different	830:838	arg1	points					845:850	different time points	830:850	different time points	830:850	Morphological changes were subsequently measured by microscopy at different time points.					
32739918	9	51	dep	E.	1525:1526	arg1	multilocularis					1528:1541	multilocularis	1528:1541	multilocularis	1528:1541	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	8	52	dep	in	1313:1314	arg1	vitro					1316:1320	vitro	1316:1320	vitro	1316:1320	RESULTS Our experiments demonstrated that the parasite vesicular structure can be directly destroyed by adding galactosidases into the in vitro culture system, resulting in the fact that the parasite metacestode vesicles could not anymore infect and develop in mice after this glycan digestion.					
32739918	8	53	theme	vesicular	1233:1241	arg1	structure					1243:1251	the parasite vesicular structure	1220:1251	the parasite vesicular structure	1220:1251	RESULTS Our experiments demonstrated that the parasite vesicular structure can be directly destroyed by adding galactosidases into the in vitro culture system, resulting in the fact that the parasite metacestode vesicles could not anymore infect and develop in mice after this glycan digestion.					
32739918	10	54	theme	aborting	1985:1992	arg1	effect					1995:2000	this "aborting" effect	1979:2000	this "aborting" effect	1979:2000	DISCUSSION We raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection.					
32739918	0	55	dep	Digest	0:5	arg1	Kill					18:21	Kill	18:21	Kill the Parasite: A New Experimental Concept in Treating Alveolar Echinococcosis	18:98	Digest the Sugar, Kill the Parasite: A New Experimental Concept in Treating Alveolar Echinococcosis.					
32739918	7	56	theme	recovered	982:990	arg1	parasites					992:1000	finally recovered parasites	974:1000	finally recovered parasites	974:1000	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	4	57	theme	β1-3-galactosidase	730:747	arg1	addition					692:699	addition	692:699	addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium	692:761	METHODS Experimentally, E. multilocularis (clone H95) metacestodes were cultured in vitro with or without addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium.					
32739918	9	58	theme	lower	1723:1727	arg1	secretion					1735:1743	a lower IL-10 secretion	1721:1743	a lower IL-10 secretion from CD4+ Teffs	1721:1759	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	0	59	theme	New	39:41	arg1	Concept					56:62	A New Experimental Concept	37:62	the Parasite: A New Experimental Concept	23:62	Digest the Sugar, Kill the Parasite: A New Experimental Concept in Treating Alveolar Echinococcosis.					
32739918	3	60	theme	kill	539:542	arg1	or					536:537	or	536:537	or kill	536:542	This should allow the host defense to more easily recognize and attack (or kill) the parasite by immune-mediated effects.					
32739918	9	61	theme	CD4+	1831:1834	arg1	Teff					1836:1839	CD4+ Teff	1831:1839	CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase	1831:1935	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	8	62	theme	glycan	1455:1460	arg1	digestion					1462:1470	this glycan digestion	1450:1470	this glycan digestion	1450:1470	RESULTS Our experiments demonstrated that the parasite vesicular structure can be directly destroyed by adding galactosidases into the in vitro culture system, resulting in the fact that the parasite metacestode vesicles could not anymore infect and develop in mice after this glycan digestion.					
32739918	7	63	theme	flow	1162:1165	arg1	cytometry					1167:1175	flow cytometry	1162:1175	flow cytometry	1162:1175	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	1	64	theme	physiological	299:311	arg1	reactions					336:344	physiological and immunological host reactions	299:344	physiological and immunological host reactions	299:344	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	9	65	theme	CD4+	1750:1753	arg1	Teffs					1755:1759	CD4+ Teffs	1750:1759	CD4+ Teffs	1750:1759	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	4	66	theme	clone	629:633	arg1	metacestodes					640:651	E. multilocularis (clone H95) metacestodes	610:651	E. multilocularis (clone H95) metacestodes	610:651	METHODS Experimentally, E. multilocularis (clone H95) metacestodes were cultured in vitro with or without addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium.					
32739918	4	66	theme	clone	629:633	arg1	H95					635:637	clone H95	629:637	clone H95	629:637	METHODS Experimentally, E. multilocularis (clone H95) metacestodes were cultured in vitro with or without addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium.					
32739918	10	67	theme	altered	2022:2028	arg1	PD1					2030:2032	an altered PD1 and IL-10 response fine-tuning	2019:2063	PD1	2030:2032	DISCUSSION We raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection.					
32739918	1	68	theme	immunological	317:329	arg1	reactions					336:344	physiological and immunological host reactions	299:344	physiological and immunological host reactions	299:344	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	1	69	dep	E.	118:119	arg1	multilocularis					121:134	multilocularis	121:134	multilocularis	121:134	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	7	70	theme	key	1132:1134	arg1	levels					1102:1107	host-related PD1 and IL-10 levels	1075:1107	host-related PD1 and IL-10 levels	1075:1107	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	7	70	theme	key	1132:1134	arg1	immunoregulators					1136:1151	the key immunoregulators	1128:1151	the key immunoregulators	1128:1151	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	1	71	dep	INTRODUCTION	101:112	arg1	LL					153:154	LL	153:154	LL	153:154	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	1	71	dep	INTRODUCTION	101:112	arg1	layer					146:150	The E. multilocularis laminated layer	114:150	INTRODUCTION The E. multilocularis laminated layer (LL)	101:155	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	2	72	theme	experimental	364:375	arg1	design					377:382	an experimental design	361:382	an experimental design with the idea to modify the (glycan) surface of the LL	361:437	We elaborated an experimental design with the idea to modify the (glycan) surface of the LL by a targeted digestion.					
32739918	9	73	dep	E.	1867:1868	arg1	multilocularis					1870:1883	multilocularis	1870:1883	multilocularis	1870:1883	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	11	74	theme	therapeutical	2176:2188	arg1	administration					2198:2211	therapeutical in vivo administration	2176:2211	therapeutical in vivo administration of the enzymes	2176:2226	These findings justify a continuation of these experiments upon therapeutical in vivo administration of the enzymes.					
32739918	5	75	theme	Morphological	764:776	arg1	changes					778:784	Morphological changes	764:784	Morphological changes	764:784	Morphological changes were subsequently measured by microscopy at different time points.					
32739918	9	76	theme	PD1	1789:1791	arg1	difference					1775:1784	no difference	1772:1784	no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase	1772:1935	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	8	77	theme	culture	1322:1328	arg1	system					1330:1335	the in vitro culture system	1309:1335	the in vitro culture system	1309:1335	RESULTS Our experiments demonstrated that the parasite vesicular structure can be directly destroyed by adding galactosidases into the in vitro culture system, resulting in the fact that the parasite metacestode vesicles could not anymore infect and develop in mice after this glycan digestion.					
32739918	7	78	theme	IL-10	1096:1100	arg1	levels					1102:1107	host-related PD1 and IL-10 levels	1075:1107	host-related PD1 and IL-10 levels	1075:1107	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	7	78	theme	IL-10	1096:1100	arg1	immunoregulators					1136:1151	the key immunoregulators	1128:1151	the key immunoregulators	1128:1151	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	1	79	theme	parasitic	183:191	arg1	INTRODUCTION					101:112	INTRODUCTION	101:112	INTRODUCTION The E. multilocularis laminated layer (LL)	101:155	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	1	79	theme	parasitic	183:191	arg1	structure					193:201	a heavily glycosylated parasitic structure	160:201	a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions	160:344	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	9	80	theme	IL-10	1797:1801	arg1	levels					1814:1819	IL-10 expression levels	1797:1819	IL-10 expression levels	1797:1819	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	7	81	theme	PD1	1088:1090	arg1	levels					1102:1107	host-related PD1 and IL-10 levels	1075:1107	host-related PD1 and IL-10 levels	1075:1107	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	7	81	theme	PD1	1088:1090	arg1	immunoregulators					1136:1151	the key immunoregulators	1128:1151	the key immunoregulators	1128:1151	For finally recovered parasites, the respective load was assessed ex vivo by wet weight measurement, and host-related PD1 and IL-10 levels were determined as the key immunoregulators by using flow cytometry.					
32739918	9	82	theme	levels	1814:1819	arg1	difference					1775:1784	no difference	1772:1784	no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase	1772:1935	Moreover, when compared to the mice inoculated with E. multilocularis metacestode without galactosidases, PD1 expression was upregulated in CD4+ Teffs from mice inoculated with E. multilocularis metacestode pretreated with β1-3-galactosidase, with a lower IL-10 secretion from CD4+ Teffs; there was no difference of PD1 and IL-10 expression levels regarding CD4+ Teff from mice inoculated with E. multilocularis metacestode pretreated with α1-3,4,6-galac-tosidase.					
32739918	1	83	theme	parasite	285:292	arg1	metacestode					264:274	metacestode	264:274	metacestode	264:274	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	1	83	theme	parasite	285:292	arg1	stage					257:261	the larval stage	246:261	the larval stage (metacestode) of this parasite	246:292	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
32739918	4	84	dep	E.	610:611	arg1	multilocularis					613:626	multilocularis	613:626	multilocularis	613:626	METHODS Experimentally, E. multilocularis (clone H95) metacestodes were cultured in vitro with or without addition of α1-3,4,6-galactosidase or β1-3-galactosidase in the medium.					
32739918	10	85	theme	response	2044:2051	arg1	fine-tuning					2053:2063	an altered PD1 and IL-10 response fine-tuning	2019:2063	fine-tuning	2053:2063	DISCUSSION We raised our hypothesis that this "aborting" effect may be linked to an altered PD1 and IL-10 response fine-tuning between immunopathology and immune protection.					
32739918	8	86	theme	parasite	1369:1376	arg1	vesicles					1390:1397	the parasite metacestode vesicles	1365:1397	the parasite metacestode vesicles	1365:1397	RESULTS Our experiments demonstrated that the parasite vesicular structure can be directly destroyed by adding galactosidases into the in vitro culture system, resulting in the fact that the parasite metacestode vesicles could not anymore infect and develop in mice after this glycan digestion.					
32739918	1	87	theme	important	217:225	arg1	role					227:230	an important role	214:230	an important role	214:230	INTRODUCTION The E. multilocularis laminated layer (LL) is a heavily glycosylated parasitic structure that plays an important role in protecting the larval stage (metacestode) of this parasite from physiological and immunological host reactions.					
33385920	0	0	theme	technological	80:92	arg1	properties					94:103	technological properties	80:103	technological properties	80:103	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	2	1	theme	native	363:368	arg1	cacti					357:361	cacti native	357:368	cacti native	357:368	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil were investigated.					
33385920	8	2	from	use	1269:1271	arg1	applications					1281:1292	food applications	1276:1292	food applications	1276:1292	This study highlights the potential of using cacti as a source of functional mucilages for use in food applications.					
33385920	3	3	dep	14.76	550:554	arg1	to					547:548	to	547:548	to	547:548	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	5	4	theme	characteristic	825:838	arg1	peaks					809:813	peaks	809:813	peaks	809:813	Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium.					
33385920	2	5	theme	cacti	357:361	arg1	cladodes					345:352	seven cladodes	339:352	seven cladodes of cacti native	339:368	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil were investigated.					
33385920	7	6	theme	Foaming	1114:1120	arg1	capacity					1122:1129	Foaming capacity	1114:1129	Foaming capacity	1114:1129	Foaming capacity was above 58% with stability greater than 95%.					
33385920	1	7	contain	have	122:125	arg2	potential					136:144	the high potential	127:144	the high potential to be used as a source of mucilages with different technological functions that can be widely explored	127:247	Brazilian cacti have the high potential to be used as a source of mucilages with different technological functions that can be widely explored.					
33385920	1	7	contain	have	122:125	arg1	cacti					116:120	Brazilian cacti	106:120	Brazilian cacti	106:120	Brazilian cacti have the high potential to be used as a source of mucilages with different technological functions that can be widely explored.					
33385920	2	8	theme	technological	286:298	arg1	properties					300:309	The physicochemical, structural and technological properties	250:309	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil	250:408	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil were investigated.					
33385920	6	9	dep	water	1011:1015	arg1	capacities					1035:1044	capacities	1035:1044	capacities	1035:1044	From the results, mucilages functional properties showed good water and oil retention capacities and a high emulsification capacity (100%) with stability above 83%.					
33385920	5	10	theme	due	907:909	arg1	%					903:903	14.50-38.60%	892:903	14.50-38.60%	892:903	Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium.					
33385920	5	10	theme	due	907:909	arg1	structure					881:889	semi-crystalline structure	864:889	semi-crystalline structure (14.50-38.60%)	864:904	Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium.					
33385920	3	11	theme	high	482:485	arg1	contents					487:494	high contents	482:494	high contents of carbohydrates (39.77 to 87.68%)	482:529	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	3	12	dep	15.65	598:602	arg1	to					595:596	to	595:596	to	595:596	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	6	13	theme	functional	977:986	arg1	properties					988:997	mucilages functional properties	967:997	mucilages functional properties	967:997	From the results, mucilages functional properties showed good water and oil retention capacities and a high emulsification capacity (100%) with stability above 83%.					
33385920	2	14	theme	structural	271:280	arg1	properties					300:309	The physicochemical, structural and technological properties	250:309	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil	250:408	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil were investigated.					
33385920	0	15	from	biodiversity	34:45	arg1	Mucilages					0:8	Mucilages	0:8	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.	0:104	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	0	15	from	biodiversity	34:45	arg1	physicochemical					60:74	physicochemical	60:74	physicochemical	60:74	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	0	15	from	biodiversity	34:45	arg1	Extraction					48:57	Extraction	48:57	Extraction	48:57	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	6	16	theme	high	1052:1055	arg1	%					1085:1085	100%	1082:1085	100%	1082:1085	From the results, mucilages functional properties showed good water and oil retention capacities and a high emulsification capacity (100%) with stability above 83%.					
33385920	6	16	theme	high	1052:1055	arg1	capacity					1072:1079	a high emulsification capacity	1050:1079	a high emulsification capacity (100%)	1050:1086	From the results, mucilages functional properties showed good water and oil retention capacities and a high emulsification capacity (100%) with stability above 83%.					
33385920	3	17	theme	high	453:456	arg1	%					478:478	8.9 to 21.54%	466:478	8.9 to 21.54%	466:478	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	3	17	theme	high	453:456	arg1	yields					458:463	high yields	453:463	high yields (8.9 to 21.54%)	453:479	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	5	18	with	polysaccharides	843:857	arg1	%					903:903	14.50-38.60%	892:903	14.50-38.60%	892:903	Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium.					
33385920	5	18	with	polysaccharides	843:857	arg1	structure					881:889	semi-crystalline structure	864:889	semi-crystalline structure (14.50-38.60%)	864:904	Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium.					
33385920	4	19	theme	monosaccharide	649:662	arg1	composition					664:674	a diverse monosaccharide composition	639:674	a diverse monosaccharide composition	639:674	All species evaluated presented a diverse monosaccharide composition, mainly constituted of galactose and arabinose, suggesting the presence of arabinogalactans as the main polysaccharides.					
33385920	6	20	theme	good	1006:1009	arg1	water					1011:1015	good water	1006:1015	good water	1006:1015	From the results, mucilages functional properties showed good water and oil retention capacities and a high emulsification capacity (100%) with stability above 83%.					
33385920	4	21	theme	main	775:778	arg1	presence					739:746	the presence	735:746	the presence of arabinogalactans	735:766	All species evaluated presented a diverse monosaccharide composition, mainly constituted of galactose and arabinose, suggesting the presence of arabinogalactans as the main polysaccharides.					
33385920	4	21	theme	main	775:778	arg1	polysaccharides					780:794	the main polysaccharides	771:794	the main polysaccharides	771:794	All species evaluated presented a diverse monosaccharide composition, mainly constituted of galactose and arabinose, suggesting the presence of arabinogalactans as the main polysaccharides.					
33385920	2	22	theme	mucilages	314:322	arg1	properties					300:309	The physicochemical, structural and technological properties	250:309	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil	250:408	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil were investigated.					
33385920	7	23	with	%	1143:1143	arg1	stability					1150:1158	stability	1150:1158	stability	1150:1158	Foaming capacity was above 58% with stability greater than 95%.					
33385920	2	24	theme	semi-arid	379:387	arg1	region					389:394	the semi-arid region	375:394	the semi-arid region of the Brazil	375:408	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil were investigated.					
33385920	4	25	theme	diverse	641:647	arg1	composition					664:674	a diverse monosaccharide composition	639:674	a diverse monosaccharide composition	639:674	All species evaluated presented a diverse monosaccharide composition, mainly constituted of galactose and arabinose, suggesting the presence of arabinogalactans as the main polysaccharides.					
33385920	1	26	theme	Brazilian	106:114	arg1	cacti					116:120	Brazilian cacti	106:120	Brazilian cacti	106:120	Brazilian cacti have the high potential to be used as a source of mucilages with different technological functions that can be widely explored.					
33385920	0	27	theme	cacti	13:17	arg1	Mucilages					0:8	Mucilages	0:8	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.	0:104	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	0	27	theme	cacti	13:17	arg1	physicochemical					60:74	physicochemical	60:74	physicochemical	60:74	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	0	27	theme	cacti	13:17	arg1	Extraction					48:57	Extraction	48:57	Extraction	48:57	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	8	28	theme	functional	1244:1253	arg1	mucilages					1255:1263	functional mucilages	1244:1263	functional mucilages	1244:1263	This study highlights the potential of using cacti as a source of functional mucilages for use in food applications.					
33385920	6	29	theme	oil	1021:1023	arg1	retention					1025:1033	oil retention	1021:1033	oil retention	1021:1033	From the results, mucilages functional properties showed good water and oil retention capacities and a high emulsification capacity (100%) with stability above 83%.					
33385920	1	30	theme	different	187:195	arg1	functions					211:219	different technological functions	187:219	different technological functions	187:219	Brazilian cacti have the high potential to be used as a source of mucilages with different technological functions that can be widely explored.					
33385920	6	31	theme	mucilages	967:975	arg1	properties					988:997	mucilages functional properties	967:997	mucilages functional properties	967:997	From the results, mucilages functional properties showed good water and oil retention capacities and a high emulsification capacity (100%) with stability above 83%.					
33385920	1	32	theme	mucilages	172:180	arg1	source					162:167	a source	160:167	a source of mucilages with different technological functions that can be widely explored	160:247	Brazilian cacti have the high potential to be used as a source of mucilages with different technological functions that can be widely explored.					
33385920	1	33	theme	technological	197:209	arg1	functions					211:219	different technological functions	187:219	different technological functions	187:219	Brazilian cacti have the high potential to be used as a source of mucilages with different technological functions that can be widely explored.					
33385920	0	34	theme	Brazilian	24:32	arg1	biodiversity					34:45	Brazilian biodiversity	24:45	Brazilian biodiversity	24:45	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	8	35	theme	food	1276:1279	arg1	applications					1281:1292	food applications	1276:1292	food applications	1276:1292	This study highlights the potential of using cacti as a source of functional mucilages for use in food applications.					
33385920	2	36	theme	Brazil	403:408	arg1	region					389:394	the semi-arid region	375:394	the semi-arid region of the Brazil	375:408	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil were investigated.					
33385920	4	37	theme	arabinogalactans	751:766	arg1	presence					739:746	the presence	735:746	the presence of arabinogalactans	735:766	All species evaluated presented a diverse monosaccharide composition, mainly constituted of galactose and arabinose, suggesting the presence of arabinogalactans as the main polysaccharides.					
33385920	4	37	theme	arabinogalactans	751:766	arg1	polysaccharides					780:794	the main polysaccharides	771:794	the main polysaccharides	771:794	All species evaluated presented a diverse monosaccharide composition, mainly constituted of galactose and arabinose, suggesting the presence of arabinogalactans as the main polysaccharides.					
33385920	8	38	theme	mucilages	1255:1263	arg1	cacti					1223:1227	cacti	1223:1227	cacti	1223:1227	This study highlights the potential of using cacti as a source of functional mucilages for use in food applications.					
33385920	8	38	theme	mucilages	1255:1263	arg1	source					1234:1239	a source	1232:1239	a source of functional mucilages for use in food applications	1232:1292	This study highlights the potential of using cacti as a source of functional mucilages for use in food applications.					
33385920	5	39	theme	polysaccharides	843:857	arg1	characteristic					825:838	characteristic	825:838	characteristic	825:838	Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium.					
33385920	1	40	with	mucilages	172:180	arg1	functions					211:219	different technological functions	187:219	different technological functions	187:219	Brazilian cacti have the high potential to be used as a source of mucilages with different technological functions that can be widely explored.					
33385920	6	41	theme	emulsification	1057:1070	arg1	%					1085:1085	100%	1082:1085	100%	1082:1085	From the results, mucilages functional properties showed good water and oil retention capacities and a high emulsification capacity (100%) with stability above 83%.					
33385920	6	41	theme	emulsification	1057:1070	arg1	capacity					1072:1079	a high emulsification capacity	1050:1079	a high emulsification capacity (100%)	1050:1086	From the results, mucilages functional properties showed good water and oil retention capacities and a high emulsification capacity (100%) with stability above 83%.					
33385920	5	42	theme	calcium	940:946	arg1	concentration					923:935	the high concentration	914:935	the high concentration of calcium	914:946	Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium.					
33385920	5	43	theme	high	918:921	arg1	concentration					923:935	the high concentration	914:935	the high concentration of calcium	914:946	Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium.					
33385920	2	44	theme	physicochemical	254:268	arg1	properties					300:309	The physicochemical, structural and technological properties	250:309	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil	250:408	The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil were investigated.					
33385920	1	45	theme	high	131:134	arg1	potential					136:144	the high potential	127:144	the high potential to be used as a source of mucilages with different technological functions that can be widely explored	127:247	Brazilian cacti have the high potential to be used as a source of mucilages with different technological functions that can be widely explored.					
33385920	3	46	dep	21.54	473:477	arg1	to					470:471	to	470:471	to	470:471	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	3	47	dep	87.68	523:527	arg1	to					520:521	to	520:521	to	520:521	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	5	48	theme	semi-crystalline	864:879	arg1	%					903:903	14.50-38.60%	892:903	14.50-38.60%	892:903	Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium.					
33385920	5	48	theme	semi-crystalline	864:879	arg1	structure					881:889	semi-crystalline structure	864:889	semi-crystalline structure (14.50-38.60%)	864:904	Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium.					
33385920	0	49	dep	Mucilages	0:8	arg1	Mucilages					0:8	Mucilages	0:8	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.	0:104	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	0	49	dep	Mucilages	0:8	arg1	physicochemical					60:74	physicochemical	60:74	physicochemical	60:74	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	0	49	dep	Mucilages	0:8	arg1	properties					94:103	technological properties	80:103	technological properties	80:103	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	0	49	dep	Mucilages	0:8	arg1	Extraction					48:57	Extraction	48:57	Extraction	48:57	Mucilages of cacti from Brazilian biodiversity: Extraction, physicochemical and technological properties.					
33385920	3	50	theme	carbohydrates	499:511	arg1	contents					487:494	high contents	482:494	high contents of carbohydrates (39.77 to 87.68%)	482:529	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	3	50	theme	carbohydrates	499:511	arg1	minerals					563:570	minerals	563:570	minerals	563:570	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	3	50	theme	carbohydrates	499:511	arg1	%					603:603	2.90 to 15.65%	590:603	2.90 to 15.65%	590:603	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	3	50	theme	carbohydrates	499:511	arg1	Ca					586:587	Ca	586:587	Ca (2.90 to 15.65%)	586:604	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	3	50	theme	carbohydrates	499:511	arg1	%					478:478	8.9 to 21.54%	466:478	8.9 to 21.54%	466:478	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	3	50	theme	carbohydrates	499:511	arg1	%					555:555	4.27 to 14.76%	542:555	4.27 to 14.76%	542:555	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	3	50	theme	carbohydrates	499:511	arg1	proteins					532:539	proteins	532:539	proteins (4.27 to 14.76%)	532:556	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33385920	3	50	theme	carbohydrates	499:511	arg1	yields					458:463	high yields	453:463	high yields (8.9 to 21.54%)	453:479	The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%).					
33049835	9	0	theme	sensitize-tested	1076:1091	arg1	cells					1106:1110	sensitize-tested colon cancer cells HT-29 and HCT-116	1076:1128	sensitize-tested colon cancer cells HT-29 and HCT-116	1076:1128	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	9	0	theme	sensitize-tested	1076:1091	arg1	HCT-116					1122:1128	HCT-116	1122:1128	HCT-116	1122:1128	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	9	0	theme	sensitize-tested	1076:1091	arg1	HT-29					1112:1116	HT-29	1112:1116	HT-29	1112:1116	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	6	1	from	1,3-	747:750	arg1	β-d-galactopyranose					641:659	β-d-galactopyranose	641:659	β-d-galactopyranose with branches at C3 and C4	641:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	1	from	1,3-	747:750	arg1	residues					719:726	terminal fucose and galactose residues	689:726	residues	719:726	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	1	from	1,3-	747:750	arg1	residues					629:636	1,6-linked residues	618:636	1,6-linked residues of β-d-galactopyranose with branches at C3 and C4	618:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	1	from	1,3-	747:750	arg1	fucose					698:703	terminal fucose and galactose residues	689:726	fucose	698:703	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	1	from	1,3-	747:750	arg1	fragments					732:740	fragments	732:740	fragments	732:740	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	0	2	theme	laminarans	84:93	arg1	activity					52:59	radiosensitizing activity	35:59	radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc	35:126	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	0	2	theme	laminarans	84:93	arg1	Composition					0:10	Composition	0:10	Composition of polysaccharides	0:29	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	6	3	from	C3	678:679	arg1	branches					666:673	branches	666:673	branches at C3 and C4	666:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	9	4	theme	colon	1093:1097	arg1	cells					1106:1110	sensitize-tested colon cancer cells HT-29 and HCT-116	1076:1128	sensitize-tested colon cancer cells HT-29 and HCT-116	1076:1128	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	9	4	theme	colon	1093:1097	arg1	HCT-116					1122:1128	HCT-116	1122:1128	HCT-116	1122:1128	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	9	4	theme	colon	1093:1097	arg1	HT-29					1112:1116	HT-29	1112:1116	HT-29	1112:1116	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	0	5	from	Composition	0:10	arg1	Kloczc					121:126	Kloczc	121:126	Kloczc	121:126	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	2	6	theme	alga	231:234	arg1	basicrassa					242:251	the brown alga Tauya basicrassa	221:251	the brown alga Tauya basicrassa	221:251	Polysaccharide fractions of alginate, laminarans and fucoidans were obtained from the brown alga Tauya basicrassa.					
33049835	6	7	theme	1,6-linked	618:627	arg1	β-d-galactopyranose					641:659	β-d-galactopyranose	641:659	β-d-galactopyranose with branches at C3 and C4	641:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	7	theme	1,6-linked	618:627	arg1	residues					629:636	1,6-linked residues	618:636	1,6-linked residues of β-d-galactopyranose with branches at C3 and C4	618:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	2	8	theme	alginate	167:174	arg1	fractions					154:162	Polysaccharide fractions	139:162	Polysaccharide fractions of alginate, laminarans and fucoidans	139:200	Polysaccharide fractions of alginate, laminarans and fucoidans were obtained from the brown alga Tauya basicrassa.					
33049835	9	9	theme	cells	1106:1110	arg1	formation					1063:1071	colony formation	1056:1071	colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation	1056:1147	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	6	10	from	fucose	698:703	arg1	backbone					604:611	a backbone	602:611	a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues	602:781	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	7	11	theme	fucose	834:839	arg1	positions					813:821	positions 2 and 4 of fucose	813:839	positions 2 and 4 of fucose	813:839	Sulfate groups were found at positions 2 and 4 of fucose, and positions 2, 3 and 4 of galactose residues.					
33049835	7	11	theme	fucose	834:839	arg1	positions					846:854	positions 2, 3 and 4 of galactose residues	846:887	positions 2, 3 and 4 of galactose residues	846:887	Sulfate groups were found at positions 2 and 4 of fucose, and positions 2, 3 and 4 of galactose residues.					
33049835	8	12	with	2TbLS	959:963	arg1	sulfation					978:986	partial sulfation	970:986	partial sulfation (46 %) at C2, C4 and C6	970:1010	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	8	12	with	2TbLS	959:963	arg1	%					992:992	46 %	989:992	46 %	989:992	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	8	13	theme	derivative	948:957	arg1	2TbLS					959:963	the derivative 2TbLS	944:963	the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6	944:1010	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	6	14	theme	galactose	709:717	arg1	β-d-galactopyranose					641:659	β-d-galactopyranose	641:659	β-d-galactopyranose with branches at C3 and C4	641:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	14	theme	galactose	709:717	arg1	residues					719:726	terminal fucose and galactose residues	689:726	residues	719:726	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	0	15	from	activity	52:59	arg1	Kloczc					121:126	Kloczc	121:126	Kloczc	121:126	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	3	16	theme	alginate	264:271	arg1	Yields					254:259	Yields	254:259	Yields of alginate and laminarans	254:286	Yields of alginate and laminarans were large (19.7 % and 5.62 %, respectively), whereas the content of fucoidans (0.52 %) was not significant.					
33049835	3	17	theme	fucoidans	357:365	arg1	content					346:352	the content	342:352	the content of fucoidans (0.52 %)	342:374	Yields of alginate and laminarans were large (19.7 % and 5.62 %, respectively), whereas the content of fucoidans (0.52 %) was not significant.					
33049835	3	17	theme	fucoidans	357:365	arg1	significant					384:394	significant	384:394	significant	384:394	Yields of alginate and laminarans were large (19.7 % and 5.62 %, respectively), whereas the content of fucoidans (0.52 %) was not significant.					
33049835	2	18	theme	Polysaccharide	139:152	arg1	fractions					154:162	Polysaccharide fractions	139:162	Polysaccharide fractions of alginate, laminarans and fucoidans	139:200	Polysaccharide fractions of alginate, laminarans and fucoidans were obtained from the brown alga Tauya basicrassa.					
33049835	6	19	with	β-d-galactopyranose	641:659	arg1	branches					666:673	branches	666:673	branches at C3 and C4	666:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	20	theme	fraction	550:557	arg1	1TbF1					559:563	The fucoidan fraction 1TbF1	537:563	The fucoidan fraction 1TbF1	537:563	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	20	theme	fraction	550:557	arg1	fucogalactan					578:589	sulfated fucogalactan	569:589	sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues	569:781	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	21	from	C4	685:686	arg1	branches					666:673	branches	666:673	branches at C3 and C4	666:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	5	22	theme	low-	481:484	arg1	polysaccharides					520:534	low- and medium-sulfated heterogeneous polysaccharides	481:534	low- and medium-sulfated heterogeneous polysaccharides	481:534	Fucoidans were low- and medium-sulfated heterogeneous polysaccharides.					
33049835	7	23	theme	residues	880:887	arg1	positions					813:821	positions 2 and 4 of fucose	813:839	positions 2 and 4 of fucose	813:839	Sulfate groups were found at positions 2 and 4 of fucose, and positions 2, 3 and 4 of galactose residues.					
33049835	7	23	theme	residues	880:887	arg1	positions					846:854	positions 2, 3 and 4 of galactose residues	846:887	positions 2, 3 and 4 of galactose residues	846:887	Sulfate groups were found at positions 2 and 4 of fucose, and positions 2, 3 and 4 of galactose residues.					
33049835	7	24	located	found	804:808	arg1	positions					813:821	positions 2 and 4 of fucose	813:839	positions 2 and 4 of fucose	813:839	Sulfate groups were found at positions 2 and 4 of fucose, and positions 2, 3 and 4 of galactose residues.					
33049835	7	24	located	found	804:808	arg2	groups					792:797	Sulfate groups	784:797	Sulfate groups	784:797	Sulfate groups were found at positions 2 and 4 of fucose, and positions 2, 3 and 4 of galactose residues.					
33049835	7	24	located	found	804:808	arg1	positions					846:854	positions 2, 3 and 4 of galactose residues	846:887	positions 2, 3 and 4 of galactose residues	846:887	Sulfate groups were found at positions 2 and 4 of fucose, and positions 2, 3 and 4 of galactose residues.					
33049835	6	25	theme	terminal	689:696	arg1	fucose					698:703	terminal fucose and galactose residues	689:726	fucose	698:703	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	8	26	theme	partial	970:976	arg1	sulfation					978:986	partial sulfation	970:986	partial sulfation (46 %) at C2, C4 and C6	970:1010	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	8	26	theme	partial	970:976	arg1	%					992:992	46 %	989:992	46 %	989:992	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	6	27	theme	1,2-fucose	763:772	arg1	residues					774:781	1,2-fucose residues	763:781	1,2-fucose residues	763:781	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	9	28	theme	cancer	1099:1104	arg1	cells					1106:1110	sensitize-tested colon cancer cells HT-29 and HCT-116	1076:1128	sensitize-tested colon cancer cells HT-29 and HCT-116	1076:1128	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	9	28	theme	cancer	1099:1104	arg1	HCT-116					1122:1128	HCT-116	1122:1128	HCT-116	1122:1128	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	9	28	theme	cancer	1099:1104	arg1	HT-29					1112:1116	HT-29	1112:1116	HT-29	1112:1116	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	4	29	contain	had	421:423	arg2	structures					433:442	typical structures	425:442	typical structures for those substances	425:463	Alginate and laminarans had typical structures for those substances.					
33049835	4	29	contain	had	421:423	arg1	Alginate					397:404	Alginate	397:404	Alginate	397:404	Alginate and laminarans had typical structures for those substances.					
33049835	4	29	contain	had	421:423	arg1	laminarans					410:419	laminarans	410:419	laminarans	410:419	Alginate and laminarans had typical structures for those substances.					
33049835	0	30	theme	polysaccharides	15:29	arg1	activity					52:59	radiosensitizing activity	35:59	radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc	35:126	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	0	30	theme	polysaccharides	15:29	arg1	Composition					0:10	Composition	0:10	Composition of polysaccharides	0:29	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	2	31	theme	fucoidans	192:200	arg1	fractions					154:162	Polysaccharide fractions	139:162	Polysaccharide fractions of alginate, laminarans and fucoidans	139:200	Polysaccharide fractions of alginate, laminarans and fucoidans were obtained from the brown alga Tauya basicrassa.					
33049835	6	32	from	residues	774:781	arg1	β-d-galactopyranose					641:659	β-d-galactopyranose	641:659	β-d-galactopyranose with branches at C3 and C4	641:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	32	from	residues	774:781	arg1	residues					719:726	terminal fucose and galactose residues	689:726	residues	719:726	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	32	from	residues	774:781	arg1	residues					629:636	1,6-linked residues	618:636	1,6-linked residues of β-d-galactopyranose with branches at C3 and C4	618:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	32	from	residues	774:781	arg1	fucose					698:703	terminal fucose and galactose residues	689:726	fucose	698:703	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	32	from	residues	774:781	arg1	fragments					732:740	fragments	732:740	fragments	732:740	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	0	33	theme	radiosensitizing	35:50	arg1	activity					52:59	radiosensitizing activity	35:59	radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc	35:126	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	3	34	theme	laminarans	277:286	arg1	Yields					254:259	Yields	254:259	Yields of alginate and laminarans	254:286	Yields of alginate and laminarans were large (19.7 % and 5.62 %, respectively), whereas the content of fucoidans (0.52 %) was not significant.					
33049835	4	35	theme	typical	425:431	arg1	structures					433:442	typical structures	425:442	typical structures for those substances	425:463	Alginate and laminarans had typical structures for those substances.					
33049835	2	36	theme	Tauya	236:240	arg1	basicrassa					242:251	the brown alga Tauya basicrassa	221:251	the brown alga Tauya basicrassa	221:251	Polysaccharide fractions of alginate, laminarans and fucoidans were obtained from the brown alga Tauya basicrassa.					
33049835	6	37	contain	containing	591:600	arg1	1TbF1					559:563	The fucoidan fraction 1TbF1	537:563	The fucoidan fraction 1TbF1	537:563	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	37	contain	containing	591:600	arg1	fucogalactan					578:589	sulfated fucogalactan	569:589	sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues	569:781	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	37	contain	containing	591:600	arg2	backbone					604:611	a backbone	602:611	a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues	602:781	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	0	38	theme	native	64:69	arg1	laminarans					84:93	native and sulfated laminarans	64:93	native and sulfated laminarans from the Tаuуа basicrassa Kloczc	64:126	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	7	39	theme	Sulfate	784:790	arg1	groups					792:797	Sulfate groups	784:797	Sulfate groups	784:797	Sulfate groups were found at positions 2 and 4 of fucose, and positions 2, 3 and 4 of galactose residues.					
33049835	3	40	dep	large	293:297	arg1	%					305:305	19.7 %	300:305	19.7 %	300:305	Yields of alginate and laminarans were large (19.7 % and 5.62 %, respectively), whereas the content of fucoidans (0.52 %) was not significant.					
33049835	3	40	dep	large	293:297	arg1	%					316:316	5.62 %	311:316	5.62 %	311:316	Yields of alginate and laminarans were large (19.7 % and 5.62 %, respectively), whereas the content of fucoidans (0.52 %) was not significant.					
33049835	6	41	from	1,4-	753:756	arg1	β-d-galactopyranose					641:659	β-d-galactopyranose	641:659	β-d-galactopyranose with branches at C3 and C4	641:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	41	from	1,4-	753:756	arg1	residues					719:726	terminal fucose and galactose residues	689:726	residues	719:726	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	41	from	1,4-	753:756	arg1	residues					629:636	1,6-linked residues	618:636	1,6-linked residues of β-d-galactopyranose with branches at C3 and C4	618:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	41	from	1,4-	753:756	arg1	fucose					698:703	terminal fucose and galactose residues	689:726	fucose	698:703	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	41	from	1,4-	753:756	arg1	fragments					732:740	fragments	732:740	fragments	732:740	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	42	link	1,6-linked	618:627	arg1	β-d-galactopyranose					641:659	β-d-galactopyranose	641:659	β-d-galactopyranose with branches at C3 and C4	641:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	42	link	1,6-linked	618:627	arg1	residues					629:636	1,6-linked residues	618:636	1,6-linked residues of β-d-galactopyranose with branches at C3 and C4	618:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	0	43	from	Kloczc	121:126	arg1	laminarans					84:93	native and sulfated laminarans	64:93	native and sulfated laminarans from the Tаuуа basicrassa Kloczc	64:126	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	0	43	from	Kloczc	121:126	arg1	activity					52:59	radiosensitizing activity	35:59	radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc	35:126	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	0	43	from	Kloczc	121:126	arg1	Composition					0:10	Composition	0:10	Composition of polysaccharides	0:29	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	2	44	theme	brown	225:229	arg1	basicrassa					242:251	the brown alga Tauya basicrassa	221:251	the brown alga Tauya basicrassa	221:251	Polysaccharide fractions of alginate, laminarans and fucoidans were obtained from the brown alga Tauya basicrassa.					
33049835	1	45	theme	et	129:130	arg1	Krupn					132:136	et Krupn	129:136	et Krupn.	129:137	et Krupn.					
33049835	9	46	dep	cells	1106:1110	arg1	cells					1106:1110	sensitize-tested colon cancer cells HT-29 and HCT-116	1076:1128	sensitize-tested colon cancer cells HT-29 and HCT-116	1076:1128	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	9	46	dep	cells	1106:1110	arg1	HCT-116					1122:1128	HCT-116	1122:1128	HCT-116	1122:1128	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	9	46	dep	cells	1106:1110	arg1	HT-29					1112:1116	HT-29	1112:1116	HT-29	1112:1116	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	9	47	theme	X-ray	1133:1137	arg1	radiation					1139:1147	X-ray radiation	1133:1147	X-ray radiation	1133:1147	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	6	48	from	residues	629:636	arg1	backbone					604:611	a backbone	602:611	a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues	602:781	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	8	49	theme	Laminaran	890:898	arg1	2TbL					900:903	Laminaran 2TbL	890:903	Laminaran 2TbL	890:903	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	7	50	theme	galactose	870:878	arg1	residues					880:887	galactose residues	870:887	galactose residues	870:887	Sulfate groups were found at positions 2 and 4 of fucose, and positions 2, 3 and 4 of galactose residues.					
33049835	9	51	theme	colony	1056:1061	arg1	formation					1063:1071	colony formation	1056:1071	colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation	1056:1147	It was shown that 2TbL and 2TbLS inhibited colony formation of sensitize-tested colon cancer cells HT-29 and HCT-116 to X-ray radiation.					
33049835	8	52	from	C6	1009:1010	arg1	sulfation					978:986	partial sulfation	970:986	partial sulfation (46 %) at C2, C4 and C6	970:1010	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	8	52	from	C6	1009:1010	arg1	%					992:992	46 %	989:992	46 %	989:992	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	0	53	theme	sulfated	75:82	arg1	laminarans					84:93	native and sulfated laminarans	64:93	native and sulfated laminarans from the Tаuуа basicrassa Kloczc	64:126	Composition of polysaccharides and radiosensitizing activity of native and sulfated laminarans from the Tаuуа basicrassa Kloczc.					
33049835	2	54	theme	laminarans	177:186	arg1	fractions					154:162	Polysaccharide fractions	139:162	Polysaccharide fractions of alginate, laminarans and fucoidans	139:200	Polysaccharide fractions of alginate, laminarans and fucoidans were obtained from the brown alga Tauya basicrassa.					
33049835	5	55	theme	medium-sulfated	490:504	arg1	polysaccharides					520:534	low- and medium-sulfated heterogeneous polysaccharides	481:534	low- and medium-sulfated heterogeneous polysaccharides	481:534	Fucoidans were low- and medium-sulfated heterogeneous polysaccharides.					
33049835	6	56	from	fragments	732:740	arg1	backbone					604:611	a backbone	602:611	a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues	602:781	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	57	theme	fucoidan	541:548	arg1	1TbF1					559:563	The fucoidan fraction 1TbF1	537:563	The fucoidan fraction 1TbF1	537:563	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	57	theme	fucoidan	541:548	arg1	fucogalactan					578:589	sulfated fucogalactan	569:589	sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues	569:781	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	8	58	from	C4	1002:1003	arg1	sulfation					978:986	partial sulfation	970:986	partial sulfation (46 %) at C2, C4 and C6	970:1010	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	8	58	from	C4	1002:1003	arg1	%					992:992	46 %	989:992	46 %	989:992	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	5	59	theme	heterogeneous	506:518	arg1	polysaccharides					520:534	low- and medium-sulfated heterogeneous polysaccharides	481:534	low- and medium-sulfated heterogeneous polysaccharides	481:534	Fucoidans were low- and medium-sulfated heterogeneous polysaccharides.					
33049835	6	60	theme	sulfated	569:576	arg1	1TbF1					559:563	The fucoidan fraction 1TbF1	537:563	The fucoidan fraction 1TbF1	537:563	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	60	theme	sulfated	569:576	arg1	fucogalactan					578:589	sulfated fucogalactan	569:589	sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues	569:781	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	61	theme	β-d-galactopyranose	641:659	arg1	β-d-galactopyranose					641:659	β-d-galactopyranose	641:659	β-d-galactopyranose with branches at C3 and C4	641:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	61	theme	β-d-galactopyranose	641:659	arg1	residues					719:726	terminal fucose and galactose residues	689:726	residues	719:726	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	61	theme	β-d-galactopyranose	641:659	arg1	residues					629:636	1,6-linked residues	618:636	1,6-linked residues of β-d-galactopyranose with branches at C3 and C4	618:686	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	61	theme	β-d-galactopyranose	641:659	arg1	fucose					698:703	terminal fucose and galactose residues	689:726	fucose	698:703	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	6	61	theme	β-d-galactopyranose	641:659	arg1	fragments					732:740	fragments	732:740	fragments	732:740	The fucoidan fraction 1TbF1 was sulfated fucogalactan containing a backbone from 1,6-linked residues of β-d-galactopyranose with branches at C3 and C4, terminal fucose and galactose residues and fragments from 1,3-; 1,4-; and 1,2-fucose residues.					
33049835	8	62	from	C2	998:999	arg1	sulfation					978:986	partial sulfation	970:986	partial sulfation (46 %) at C2, C4 and C6	970:1010	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
33049835	8	62	from	C2	998:999	arg1	%					992:992	46 %	989:992	46 %	989:992	Laminaran 2TbL was subjected to a sulfation to obtain the derivative 2TbLS with partial sulfation (46 %) at C2, C4 and C6.					
32229214	3	0	attach	attached	633:640	arg2	-α-D-Glcp-					557:566	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-α-D-Glcp-	557:566	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	0	attach	attached	633:640	arg2	chains					523:528	two branched chains	510:528	two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6	510:654	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	0	attach	attached	633:640	arg2	-β-D-Manp-					619:628	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-β-D-Manp-	619:628	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	0	attach	attached	633:640	arg1	C6					645:646	C6	645:646	C6 of →2,6	645:654	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	4	1	theme	adenine-induced	756:770	arg1	rats					801:804	adenine-induced chronic kidney disease (CKD) rats	756:804	adenine-induced chronic kidney disease (CKD) rats	756:804	Orally administrated CSMP showed renal protection function in adenine-induced chronic kidney disease (CKD) rats.					
32229214	7	2	dep	mechanisms	1079:1088	arg1	behind					1090:1095	behind	1090:1095	behind these effects	1090:1109	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	6	3	theme	acetate	1010:1016	arg1	levels					1043:1048	acetate, propionate and butyrate levels	1010:1048	acetate, propionate and butyrate levels	1010:1048	CSMP increased acetate, propionate and butyrate levels both in colon and cecum.					
32229214	1	4	theme	Cephalosporium	244:257	arg1	mycelia					268:274	Cephalosporium sinensis mycelia	244:274	Cephalosporium sinensis mycelia	244:274	In this study, a new polysaccharide (CSMP, Mw = 16,685 Da) was isolated and purified from Cephalosporium sinensis mycelia.					
32229214	7	5	theme	improvement	1261:1271	arg1	expression					1205:1214	expression	1205:1214	expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio	1205:1304	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	7	6	theme	regulatory	1273:1282	arg1	Tregs					1293:1297	Tregs	1293:1297	Tregs	1293:1297	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	7	6	theme	regulatory	1273:1282	arg1	cells					1286:1290	regulatory T cells	1273:1290	regulatory T cells (Tregs) ratio	1273:1304	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	5	7	theme	Echerichia	974:983	arg1	subgroup					985:992	Echerichia subgroup	974:992	Echerichia subgroup	974:992	Further analysis demonstrated that CSMP increased relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium, and decreased Echerichia subgroup.					
32229214	3	8	contain	has	427:429	arg2	backbone					433:440	a backbone	431:440	a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→	431:691	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	8	contain	has	427:429	arg1	CSMP					422:425	CSMP	422:425	CSMP	422:425	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	7	9	theme	factor	1159:1164	arg1	level					1182:1186	the down-regulation nuclear factor kappa-B (NF-κB) level	1131:1186	the down-regulation nuclear factor kappa-B (NF-κB) level	1131:1186	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	1	10	theme	sinensis	259:266	arg1	mycelia					268:274	Cephalosporium sinensis mycelia	244:274	Cephalosporium sinensis mycelia	244:274	In this study, a new polysaccharide (CSMP, Mw = 16,685 Da) was isolated and purified from Cephalosporium sinensis mycelia.					
32229214	0	11	from	Isolation	0:8	arg1	rats					148:151	adenine-induced CKD rats	128:151	adenine-induced CKD rats	128:151	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	0	11	from	Isolation	0:8	arg1	mycelia					86:92	Cephalosporium sinensis mycelia	62:92	Cephalosporium sinensis mycelia	62:92	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	4	12	theme	kidney	780:785	arg1	CKD					796:798	CKD	796:798	CKD	796:798	Orally administrated CSMP showed renal protection function in adenine-induced chronic kidney disease (CKD) rats.					
32229214	4	12	theme	kidney	780:785	arg1	disease					787:793	chronic kidney disease	772:793	adenine-induced chronic kidney disease (CKD) rats	756:804	Orally administrated CSMP showed renal protection function in adenine-induced chronic kidney disease (CKD) rats.					
32229214	5	13	theme	group	933:937	arg1	abundance					866:874	relative abundance	857:874	relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium	857:957	Further analysis demonstrated that CSMP increased relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium, and decreased Echerichia subgroup.					
32229214	3	14	theme	branched	514:521	arg1	-α-D-Glcp-					557:566	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-α-D-Glcp-	557:566	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	14	theme	branched	514:521	arg1	-β-D-Manp-					619:628	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-β-D-Manp-	619:628	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	14	theme	branched	514:521	arg1	chains					523:528	two branched chains	510:528	two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6	510:654	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	0	15	theme	anti-nephritic	102:115	arg1	effects					117:123	its anti-nephritic effects	98:123	its anti-nephritic effects in adenine-induced CKD rats	98:151	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	4	16	from	function	744:751	arg1	rats					801:804	adenine-induced chronic kidney disease (CKD) rats	756:804	adenine-induced chronic kidney disease (CKD) rats	756:804	Orally administrated CSMP showed renal protection function in adenine-induced chronic kidney disease (CKD) rats.					
32229214	4	17	theme	chronic	772:778	arg1	CKD					796:798	CKD	796:798	CKD	796:798	Orally administrated CSMP showed renal protection function in adenine-induced chronic kidney disease (CKD) rats.					
32229214	4	17	theme	chronic	772:778	arg1	disease					787:793	chronic kidney disease	772:793	adenine-induced chronic kidney disease (CKD) rats	756:804	Orally administrated CSMP showed renal protection function in adenine-induced chronic kidney disease (CKD) rats.					
32229214	5	18	theme	genera	883:888	arg1	group					904:908	the genera Lactobacillus group	879:908	the genera Lactobacillus group	879:908	Further analysis demonstrated that CSMP increased relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium, and decreased Echerichia subgroup.					
32229214	0	19	theme	adenine-induced	128:142	arg1	rats					148:151	adenine-induced CKD rats	128:151	adenine-induced CKD rats	128:151	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	4	20	theme	renal	727:731	arg1	function					744:751	renal protection function	727:751	renal protection function in adenine-induced chronic kidney disease (CKD) rats	727:804	Orally administrated CSMP showed renal protection function in adenine-induced chronic kidney disease (CKD) rats.					
32229214	2	21	theme	composition	292:302	arg1	analysis					304:311	Monosaccharide composition analysis	277:311	Monosaccharide composition analysis	277:311	Monosaccharide composition analysis indicated that CSMP consists of mannose, glucose and galactose.					
32229214	1	22	theme	new	171:173	arg1	Mw = 16,685 Da					197:210	Mw = 16,685 Da	197:210	Mw = 16,685 Da	197:210	In this study, a new polysaccharide (CSMP, Mw = 16,685 Da) was isolated and purified from Cephalosporium sinensis mycelia.					
32229214	1	22	theme	new	171:173	arg1	polysaccharide					175:188	a new polysaccharide	169:188	a new polysaccharide (CSMP, Mw = 16,685 Da)	169:211	In this study, a new polysaccharide (CSMP, Mw = 16,685 Da) was isolated and purified from Cephalosporium sinensis mycelia.					
32229214	2	23	theme	Monosaccharide	277:290	arg1	analysis					304:311	Monosaccharide composition analysis	277:311	Monosaccharide composition analysis	277:311	Monosaccharide composition analysis indicated that CSMP consists of mannose, glucose and galactose.					
32229214	3	24	theme	detailed	379:386	arg1	analysis					399:406	A detailed structural analysis	377:406	A detailed structural analysis	377:406	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	8	25	theme	potential	1424:1432	arg1	drugs					1434:1438	the potential drugs	1420:1438	the potential drugs	1420:1438	These results indicated that CSMP could be developed as one of the potential drugs in the treatment of CKD.					
32229214	8	26	theme	drugs	1434:1438	arg1	one					1413:1415	one	1413:1415	one	1413:1415	These results indicated that CSMP could be developed as one of the potential drugs in the treatment of CKD.					
32229214	8	26	theme	drugs	1434:1438	arg1	CSMP					1386:1389	CSMP	1386:1389	CSMP	1386:1389	These results indicated that CSMP could be developed as one of the potential drugs in the treatment of CKD.					
32229214	8	26	theme	drugs	1434:1438	arg1	drugs					1434:1438	the potential drugs	1420:1438	the potential drugs	1420:1438	These results indicated that CSMP could be developed as one of the potential drugs in the treatment of CKD.					
32229214	3	27	theme	α-D-Manp-	543:551	arg1	-α-D-Glcp-					557:566	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-α-D-Glcp-	557:566	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	7	28	theme	protein-coupled	1221:1235	arg1	receptor					1237:1244	G protein-coupled receptor 41	1219:1247	G protein-coupled receptor 41 (GPR41)	1219:1255	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	7	28	theme	protein-coupled	1221:1235	arg1	GPR41					1250:1254	GPR41	1250:1254	GPR41	1250:1254	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	7	29	theme	deacetylase	1328:1338	arg1	activity					1347:1354	histone deacetylase (HDAC) activity	1320:1354	histone deacetylase (HDAC) activity	1320:1354	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	4	30	theme	protection	733:742	arg1	function					744:751	renal protection function	727:751	renal protection function in adenine-induced chronic kidney disease (CKD) rats	727:804	Orally administrated CSMP showed renal protection function in adenine-induced chronic kidney disease (CKD) rats.					
32229214	5	31	theme	Bifidobacterium	943:957	arg1	abundance					866:874	relative abundance	857:874	relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium	857:957	Further analysis demonstrated that CSMP increased relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium, and decreased Echerichia subgroup.					
32229214	7	32	theme	receptor	1237:1244	arg1	expression					1205:1214	expression	1205:1214	expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio	1205:1304	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	3	33	theme	structural	388:397	arg1	analysis					399:406	A detailed structural analysis	377:406	A detailed structural analysis	377:406	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	34	theme	-β-D-Manp-	656:665	arg1	1→					690:691	-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→	484:691	→2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→	456:691	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	7	35	theme	T	1284:1284	arg1	Tregs					1293:1297	Tregs	1293:1297	Tregs	1293:1297	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	7	35	theme	T	1284:1284	arg1	cells					1286:1290	regulatory T cells	1273:1290	regulatory T cells (Tregs) ratio	1273:1304	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	7	36	theme	cells	1286:1290	arg1	ratio					1300:1304	regulatory T cells (Tregs) ratio	1273:1304	regulatory T cells (Tregs) ratio	1273:1304	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	5	37	theme	relative	857:864	arg1	abundance					866:874	relative abundance	857:874	relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium	857:957	Further analysis demonstrated that CSMP increased relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium, and decreased Echerichia subgroup.					
32229214	0	38	theme	structural	11:20	arg1	characterization					22:37	structural characterization	11:37	structural characterization of polysaccharide from Cephalosporium sinensis mycelia	11:92	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	3	39	dep	-β-D-Manp-	619:628	arg1	1→2					615:617	1→2	615:617	1→2	615:617	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	0	40	theme	CKD	144:146	arg1	rats					148:151	adenine-induced CKD rats	128:151	adenine-induced CKD rats	128:151	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	3	41	theme	-α-D-Glcp-	589:598	arg1	-β-D-Manp-					619:628	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-β-D-Manp-	619:628	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	7	42	theme	kappa-B	1166:1172	arg1	level					1182:1186	the down-regulation nuclear factor kappa-B (NF-κB) level	1131:1186	the down-regulation nuclear factor kappa-B (NF-κB) level	1131:1186	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	0	43	theme	polysaccharide	42:55	arg1	effects					117:123	its anti-nephritic effects	98:123	its anti-nephritic effects in adenine-induced CKD rats	98:151	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	0	43	theme	polysaccharide	42:55	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	0	43	theme	polysaccharide	42:55	arg1	characterization					22:37	structural characterization	11:37	structural characterization of polysaccharide from Cephalosporium sinensis mycelia	11:92	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	3	44	theme	→2,6	651:654	arg1	C6					645:646	C6	645:646	C6 of →2,6	645:654	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	5	45	theme	Clostridium	911:921	arg1	group					933:937	Clostridium coccoides group	911:937	Clostridium coccoides group	911:937	Further analysis demonstrated that CSMP increased relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium, and decreased Echerichia subgroup.					
32229214	0	46	from	mycelia	86:92	arg1	effects					117:123	its anti-nephritic effects	98:123	its anti-nephritic effects in adenine-induced CKD rats	98:151	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	0	46	from	mycelia	86:92	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	0	46	from	mycelia	86:92	arg1	characterization					22:37	structural characterization	11:37	structural characterization of polysaccharide from Cephalosporium sinensis mycelia	11:92	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	0	46	from	mycelia	86:92	arg1	polysaccharide					42:55	polysaccharide	42:55	polysaccharide from Cephalosporium sinensis mycelia	42:92	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	5	47	theme	coccoides	923:931	arg1	group					933:937	Clostridium coccoides group	911:937	Clostridium coccoides group	911:937	Further analysis demonstrated that CSMP increased relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium, and decreased Echerichia subgroup.					
32229214	0	48	theme	Cephalosporium	62:75	arg1	mycelia					86:92	Cephalosporium sinensis mycelia	62:92	Cephalosporium sinensis mycelia	62:92	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	7	49	theme	down-regulation	1135:1149	arg1	level					1182:1186	the down-regulation nuclear factor kappa-B (NF-κB) level	1131:1186	the down-regulation nuclear factor kappa-B (NF-κB) level	1131:1186	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	3	50	theme	1→6	553:555	arg1	-α-D-Glcp-					557:566	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-α-D-Glcp-	557:566	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	51	dep	-β-D-Manp-	656:665	arg1	-α-D-Glcp-					557:566	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-α-D-Glcp-	557:566	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	51	dep	-β-D-Manp-	656:665	arg1	1→					667:668	1→	667:668	1→	667:668	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	51	dep	-β-D-Manp-	656:665	arg1	→3,6					674:677	→3,6	674:677	→3,6	674:677	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	51	dep	-β-D-Manp-	656:665	arg1	-β-D-Manp-					619:628	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-β-D-Manp-	619:628	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	51	dep	-β-D-Manp-	656:665	arg1	chains					523:528	two branched chains	510:528	two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6	510:654	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	51	dep	-β-D-Manp-	656:665	arg1	1→					495:496	1→	495:496	1→	495:496	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	4	52	theme	disease	787:793	arg1	rats					801:804	adenine-induced chronic kidney disease (CKD) rats	756:804	adenine-induced chronic kidney disease (CKD) rats	756:804	Orally administrated CSMP showed renal protection function in adenine-induced chronic kidney disease (CKD) rats.					
32229214	3	53	theme	-β-D-Galp-	604:613	arg1	-β-D-Manp-					619:628	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-β-D-Manp-	619:628	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	4	54	theme	administrated	701:713	arg1	CSMP					715:718	Orally administrated CSMP	694:718	Orally administrated CSMP	694:718	Orally administrated CSMP showed renal protection function in adenine-induced chronic kidney disease (CKD) rats.					
32229214	3	55	theme	1→4	585:587	arg1	-β-D-Manp-					619:628	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-β-D-Manp-	619:628	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	8	56	theme	CKD	1460:1462	arg1	treatment					1447:1455	the treatment	1443:1455	the treatment of CKD	1443:1462	These results indicated that CSMP could be developed as one of the potential drugs in the treatment of CKD.					
32229214	3	57	dep	-β-D-Manp-	461:470	arg1	→3,6					479:482	→3,6	479:482	→3,6	479:482	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	57	dep	-β-D-Manp-	461:470	arg1	1→					690:691	-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→	484:691	→2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→	456:691	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	57	dep	-β-D-Manp-	461:470	arg1	1→					472:473	1→	472:473	1→	472:473	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	3	58	theme	α-D-Glcp-	575:583	arg1	-β-D-Manp-					619:628	α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→	543:631	-β-D-Manp-	619:628	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
32229214	7	59	dep	receptor	1237:1244	arg1	ratio					1300:1304	regulatory T cells (Tregs) ratio	1273:1304	regulatory T cells (Tregs) ratio	1273:1304	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	5	60	theme	Further	807:813	arg1	analysis					815:822	Further analysis	807:822	Further analysis	807:822	Further analysis demonstrated that CSMP increased relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium, and decreased Echerichia subgroup.					
32229214	7	61	theme	histone	1320:1326	arg1	HDAC					1341:1344	HDAC	1341:1344	HDAC	1341:1344	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	7	61	theme	histone	1320:1326	arg1	deacetylase					1328:1338	histone deacetylase	1320:1338	histone deacetylase (HDAC) activity	1320:1354	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	0	62	from	effects	117:123	arg1	rats					148:151	adenine-induced CKD rats	128:151	adenine-induced CKD rats	128:151	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	0	62	from	effects	117:123	arg1	mycelia					86:92	Cephalosporium sinensis mycelia	62:92	Cephalosporium sinensis mycelia	62:92	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	5	63	theme	Lactobacillus	890:902	arg1	group					904:908	the genera Lactobacillus group	879:908	the genera Lactobacillus group	879:908	Further analysis demonstrated that CSMP increased relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium, and decreased Echerichia subgroup.					
32229214	6	64	theme	butyrate	1034:1041	arg1	levels					1043:1048	acetate, propionate and butyrate levels	1010:1048	acetate, propionate and butyrate levels	1010:1048	CSMP increased acetate, propionate and butyrate levels both in colon and cecum.					
32229214	5	65	theme	group	904:908	arg1	abundance					866:874	relative abundance	857:874	relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium	857:957	Further analysis demonstrated that CSMP increased relative abundance of the genera Lactobacillus group, Clostridium coccoides group and Bifidobacterium, and decreased Echerichia subgroup.					
32229214	6	66	theme	propionate	1019:1028	arg1	levels					1043:1048	acetate, propionate and butyrate levels	1010:1048	acetate, propionate and butyrate levels	1010:1048	CSMP increased acetate, propionate and butyrate levels both in colon and cecum.					
32229214	0	67	theme	sinensis	77:84	arg1	mycelia					86:92	Cephalosporium sinensis mycelia	62:92	Cephalosporium sinensis mycelia	62:92	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	0	68	from	characterization	22:37	arg1	rats					148:151	adenine-induced CKD rats	128:151	adenine-induced CKD rats	128:151	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	0	68	from	characterization	22:37	arg1	mycelia					86:92	Cephalosporium sinensis mycelia	62:92	Cephalosporium sinensis mycelia	62:92	Isolation, structural characterization of polysaccharide from Cephalosporium sinensis mycelia and its anti-nephritic effects in adenine-induced CKD rats.					
32229214	7	69	theme	NF-κB	1175:1179	arg1	level					1182:1186	the down-regulation nuclear factor kappa-B (NF-κB) level	1131:1186	the down-regulation nuclear factor kappa-B (NF-κB) level	1131:1186	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	7	70	theme	nuclear	1151:1157	arg1	level					1182:1186	the down-regulation nuclear factor kappa-B (NF-κB) level	1131:1186	the down-regulation nuclear factor kappa-B (NF-κB) level	1131:1186	The mechanisms behind these effects could be related to the down-regulation nuclear factor kappa-B (NF-κB) level by up-regulating expression of G protein-coupled receptor 41 (GPR41) and improvement regulatory T cells (Tregs) ratio by inhibiting histone deacetylase (HDAC) activity.					
32229214	3	71	theme	-β-D-Manp-	679:688	arg1	1→					690:691	-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→	484:691	→2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→	456:691	A detailed structural analysis revealed that CSMP has a backbone consisting of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→, as well as two branched chains including of α-D-Manp-(1→6)-α-D-Glcp-(1→ and α-D-Glcp-(1→4)-α-D-Glcp-(1→3)-β-D-Galp-(1→2)-β-D-Manp-(1→ attached to C6 of →2,6)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→.					
34973757	0	0	theme	immunomodulatory	79:94	arg1	activity					96:103	its immunomodulatory activity	75:103	its immunomodulatory activity	75:103	Structure identification of a polysaccharide in mushroom Lingzhi spore and its immunomodulatory activity.					
34973757	6	1	theme	1 → 6	687:691	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	1	2	theme	functional	170:179	arg1	spore					124:128	Ganoderma lucidum spore	106:128	Ganoderma lucidum spore	106:128	Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia.					
34973757	1	2	theme	functional	170:179	arg1	food					181:184	a well-known immunomodulatory functional food	140:184	a well-known immunomodulatory functional food in Asia	140:192	Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia.					
34973757	6	3	theme	1 → 3	646:650	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	0	4	from	identification	10:23	arg1	spore					65:69	mushroom Lingzhi spore	48:69	mushroom Lingzhi spore	48:69	Structure identification of a polysaccharide in mushroom Lingzhi spore and its immunomodulatory activity.					
34973757	0	4	from	identification	10:23	arg1	activity					96:103	its immunomodulatory activity	75:103	its immunomodulatory activity	75:103	Structure identification of a polysaccharide in mushroom Lingzhi spore and its immunomodulatory activity.					
34973757	4	5	theme	lucidum	427:433	arg1	GLSP-I					442:447	G. lucidum spore (GLSP-I)	424:448	G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa	424:485	In this work, the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa was isolated and purified.					
34973757	1	6	from	food	181:184	arg1	Asia					189:192	Asia	189:192	Asia	189:192	Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia.					
34973757	6	7	theme	Glc-	641:644	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	2	8	from	polysaccharides	199:213	arg1	spore					229:233	G. lucidum spore	218:233	G. lucidum spore	218:233	The polysaccharides in G. lucidum spore are responsible for the claimed immunomodulatory activity.					
34973757	7	9	theme	structural	814:823	arg1	characteristics					825:839	The structural characteristics	810:839	The structural characteristics	810:839	The structural characteristics were further identified by NMR spectra.					
34973757	6	10	dep	-Glc	717:720	arg1	Glc-					682:685	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	Glc-	682:685	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	6	11	theme	1 → 3	767:771	arg1	-Glc					773:776	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	773:776	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	4	12	theme	spore	435:439	arg1	GLSP-I					442:447	G. lucidum spore (GLSP-I)	424:448	G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa	424:485	In this work, the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa was isolated and purified.					
34973757	8	13	theme	side	960:963	arg1	chains					965:970	side chains	960:970	side chains linking at O-6	960:985	The results indicated that the backbone of GLSP-I was (1 → 3)-β-D-glucan, with side chains linking at O-6.					
34973757	8	14	with	-β-D-glucan	942:952	arg1	chains					965:970	side chains	960:970	side chains linking at O-6	960:985	The results indicated that the backbone of GLSP-I was (1 → 3)-β-D-glucan, with side chains linking at O-6.					
34973757	10	15	theme	activity	1052:1059	arg1	assay					1061:1065	The immunomodulatory activity assay	1031:1065	The immunomodulatory activity assay	1031:1065	The immunomodulatory activity assay indicated that GLSP-I could activate macrophages in a dose-dependent manner.					
34973757	8	16	theme	GLSP-I	924:929	arg1	backbone					912:919	the backbone	908:919	the backbone of GLSP-I	908:929	The results indicated that the backbone of GLSP-I was (1 → 3)-β-D-glucan, with side chains linking at O-6.					
34973757	8	16	theme	GLSP-I	924:929	arg1	-β-D-glucan					942:952	-β-D-glucan	942:952	-β-D-glucan	942:952	The results indicated that the backbone of GLSP-I was (1 → 3)-β-D-glucan, with side chains linking at O-6.					
34973757	4	17	theme	water-soluble	392:404	arg1	polysaccharide					406:419	the leading water-soluble polysaccharide	380:419	the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa	380:485	In this work, the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa was isolated and purified.					
34973757	5	18	theme	monosaccharide	518:531	arg1	composition					533:543	The monosaccharide composition	514:543	The monosaccharide composition analysed by gas chromatography	514:574	The monosaccharide composition analysed by gas chromatography indicated that GLSP-I was a glucan.					
34973757	6	19	theme	-Glc-	761:765	arg1	-Glc					773:776	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	773:776	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	4	20	theme	leading	384:390	arg1	polysaccharide					406:419	the leading water-soluble polysaccharide	380:419	the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa	380:485	In this work, the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa was isolated and purified.					
34973757	6	21	theme	-Glc	676:679	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	0	22	theme	Structure	0:8	arg1	identification					10:23	Structure identification	0:23	Structure identification of a polysaccharide in mushroom Lingzhi spore and its immunomodulatory activity.	0:104	Structure identification of a polysaccharide in mushroom Lingzhi spore and its immunomodulatory activity.					
34973757	6	23	theme	1 → 3	731:735	arg1	-Glc					773:776	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	773:776	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	4	24	from	polysaccharide	406:419	arg1	GLSP-I					442:447	G. lucidum spore (GLSP-I)	424:448	G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa	424:485	In this work, the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa was isolated and purified.					
34973757	9	25	dep	drawn	1015:1019	arg1	below					1024:1028	below	1024:1028	below	1024:1028	The proposed structure was drawn as below.					
34973757	6	26	theme	1 → 3	755:759	arg1	-Glc					773:776	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	773:776	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	6	27	theme	1 → 6	711:715	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	1	28	theme	Ganoderma	106:114	arg1	lucidum					116:122	Ganoderma lucidum	106:122	Ganoderma lucidum spore	106:128	Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia.					
34973757	6	29	theme	1 → 6	670:674	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	6	30	theme	side	618:621	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	6	30	theme	side	618:621	arg1	chains					623:628	Three side chains	612:628	Three side chains	612:628	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	6	30	theme	side	618:621	arg1	-Glc					773:776	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	773:776	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	1	31	theme	lucidum	116:122	arg1	spore					124:128	Ganoderma lucidum spore	106:128	Ganoderma lucidum spore	106:128	Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia.					
34973757	1	31	theme	lucidum	116:122	arg1	food					181:184	a well-known immunomodulatory functional food	140:184	a well-known immunomodulatory functional food in Asia	140:192	Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia.					
34973757	4	32	theme	molecular	457:465	arg1	weight					467:472	a molecular weight	455:472	a molecular weight of 128.0 kDa	455:485	In this work, the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa was isolated and purified.					
34973757	6	33	theme	-Glc-	664:668	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	9	34	theme	proposed	992:999	arg1	structure					1001:1009	The proposed structure	988:1009	The proposed structure	988:1009	The proposed structure was drawn as below.					
34973757	0	35	theme	polysaccharide	30:43	arg1	identification					10:23	Structure identification	0:23	Structure identification of a polysaccharide in mushroom Lingzhi spore and its immunomodulatory activity.	0:104	Structure identification of a polysaccharide in mushroom Lingzhi spore and its immunomodulatory activity.					
34973757	4	36	theme	128.0 kDa	477:485	arg1	weight					467:472	a molecular weight	455:472	a molecular weight of 128.0 kDa	455:485	In this work, the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa was isolated and purified.					
34973757	4	37	with	GLSP-I	442:447	arg1	weight					467:472	a molecular weight	455:472	a molecular weight of 128.0 kDa	455:485	In this work, the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa was isolated and purified.					
34973757	0	38	theme	Lingzhi	57:63	arg1	spore					65:69	mushroom Lingzhi spore	48:69	mushroom Lingzhi spore	48:69	Structure identification of a polysaccharide in mushroom Lingzhi spore and its immunomodulatory activity.					
34973757	6	39	theme	-Glc-	749:753	arg1	-Glc					773:776	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	773:776	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	6	40	theme	-Glc-	705:709	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	0	41	theme	mushroom	48:55	arg1	spore					65:69	mushroom Lingzhi spore	48:69	mushroom Lingzhi spore	48:69	Structure identification of a polysaccharide in mushroom Lingzhi spore and its immunomodulatory activity.					
34973757	3	42	theme	polysaccharides	333:347	arg1	information					318:328	the structural information	303:328	the structural information of polysaccharides	303:347	However, the structural information of polysaccharides remains unclear.					
34973757	2	43	theme	lucidum	221:227	arg1	spore					229:233	G. lucidum spore	218:233	G. lucidum spore	218:233	The polysaccharides in G. lucidum spore are responsible for the claimed immunomodulatory activity.					
34973757	6	44	theme	1 → 3	743:747	arg1	-Glc					773:776	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	773:776	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	3	45	theme	structural	307:316	arg1	information					318:328	the structural information	303:328	the structural information of polysaccharides	303:347	However, the structural information of polysaccharides remains unclear.					
34973757	2	46	theme	G.	218:219	arg1	spore					229:233	G. lucidum spore	218:233	G. lucidum spore	218:233	The polysaccharides in G. lucidum spore are responsible for the claimed immunomodulatory activity.					
34973757	4	47	theme	G.	424:425	arg1	GLSP-I					442:447	G. lucidum spore (GLSP-I)	424:448	G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa	424:485	In this work, the leading water-soluble polysaccharide in G. lucidum spore (GLSP-I) with a molecular weight of 128.0 kDa was isolated and purified.					
34973757	6	48	theme	1 → 3	658:662	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	10	49	theme	immunomodulatory	1035:1050	arg1	assay					1061:1065	The immunomodulatory activity assay	1031:1065	The immunomodulatory activity assay	1031:1065	The immunomodulatory activity assay indicated that GLSP-I could activate macrophages in a dose-dependent manner.					
34973757	6	50	theme	1 → 6	699:703	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	2	51	theme	immunomodulatory	267:282	arg1	activity					284:291	the claimed immunomodulatory activity	255:291	the claimed immunomodulatory activity	255:291	The polysaccharides in G. lucidum spore are responsible for the claimed immunomodulatory activity.					
34973757	7	52	theme	NMR	868:870	arg1	spectra					872:878	NMR spectra	868:878	NMR spectra	868:878	The structural characteristics were further identified by NMR spectra.					
34973757	6	53	theme	Glc-	726:729	arg1	-Glc					773:776	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	773:776	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	6	54	theme	-Glc-	737:741	arg1	-Glc					773:776	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	773:776	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	2	55	theme	claimed	259:265	arg1	activity					284:291	the claimed immunomodulatory activity	255:291	the claimed immunomodulatory activity	255:291	The polysaccharides in G. lucidum spore are responsible for the claimed immunomodulatory activity.					
34973757	6	56	theme	-Glc-	693:697	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	1	57	theme	well-known	142:151	arg1	spore					124:128	Ganoderma lucidum spore	106:128	Ganoderma lucidum spore	106:128	Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia.					
34973757	1	57	theme	well-known	142:151	arg1	food					181:184	a well-known immunomodulatory functional food	140:184	a well-known immunomodulatory functional food in Asia	140:192	Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia.					
34973757	6	58	theme	-Glc-	652:656	arg1	-Glc					717:720	Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc	641:776	-Glc	717:720	Three side chains, including Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 6)-Glc, Glc-(1 → 6)-Glc-(1 → 6)-Glc-(1 → 6)-Glc and Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc-(1 → 3)-Glc, were identified by UPLC-MS/MS.					
34973757	5	59	theme	gas	557:559	arg1	chromatography					561:574	gas chromatography	557:574	gas chromatography	557:574	The monosaccharide composition analysed by gas chromatography indicated that GLSP-I was a glucan.					
34973757	1	60	theme	immunomodulatory	153:168	arg1	spore					124:128	Ganoderma lucidum spore	106:128	Ganoderma lucidum spore	106:128	Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia.					
34973757	1	60	theme	immunomodulatory	153:168	arg1	food					181:184	a well-known immunomodulatory functional food	140:184	a well-known immunomodulatory functional food in Asia	140:192	Ganoderma lucidum spore serves as a well-known immunomodulatory functional food in Asia.					
34973757	10	61	theme	dose-dependent	1121:1134	arg1	manner					1136:1141	a dose-dependent manner	1119:1141	a dose-dependent manner	1119:1141	The immunomodulatory activity assay indicated that GLSP-I could activate macrophages in a dose-dependent manner.					
32479947	0	0	theme	novel	85:89	arg1	polysaccharide					91:104	a novel polysaccharide	83:104	a novel polysaccharide from Polygonatum sibiricum	83:131	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	5	1	theme	in	840:841	arg1	results					832:838	the preliminary screening results	806:838	the preliminary screening results in vitro	806:847	According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo.					
32479947	4	2	theme	phagocytic	763:772	arg1	activity					774:781	phagocytic activity	763:781	phagocytic activity	763:781	Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro.					
32479947	2	3	theme	ion-exchange	497:508	arg1	chromatography					510:523	DEAE-Sepharose Fast Flow ion-exchange chromatography	472:523	DEAE-Sepharose Fast Flow ion-exchange chromatography	472:523	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	6	4	theme	immunomodulatory	1003:1018	arg1	function					1020:1027	an immunomodulatory function	1000:1027	an immunomodulatory function	1000:1027	The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator.					
32479947	2	5	theme	polysaccharides	383:397	arg1	fractions					399:407	Four polysaccharides fractions	378:407	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum	378:453	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	5	theme	polysaccharides	383:397	arg1	PSP3					422:425	PSP3	422:425	PSP3	422:425	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	5	theme	polysaccharides	383:397	arg1	PSP2					416:419	PSP2	416:419	PSP2	416:419	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	5	theme	polysaccharides	383:397	arg1	PSP4					431:434	PSP4	431:434	PSP4	431:434	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	5	theme	polysaccharides	383:397	arg1	PSP1					410:413	PSP1	410:413	PSP1	410:413	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	1	6	theme	active	282:287	arg1	substances					289:298	the active substances	278:298	the active substances	278:298	The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application.					
32479947	0	7	from	Purification	0:11	arg1	sibiricum					123:131	Polygonatum sibiricum	111:131	Polygonatum sibiricum	111:131	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	2	8	dep	fractions	399:407	arg1	PSP4					431:434	PSP4	431:434	PSP4	431:434	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	8	dep	fractions	399:407	arg1	PSP3					422:425	PSP3	422:425	PSP3	422:425	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	8	dep	fractions	399:407	arg1	PSP2					416:419	PSP2	416:419	PSP2	416:419	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	8	dep	fractions	399:407	arg1	fractions					399:407	Four polysaccharides fractions	378:407	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum	378:453	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	8	dep	fractions	399:407	arg1	PSP1					410:413	PSP1	410:413	PSP1	410:413	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	1	9	theme	substances	289:298	arg1	relationships					261:273	the structure-activity relationships	238:273	the structure-activity relationships of the active substances	238:298	The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application.					
32479947	0	10	theme	polysaccharide	91:104	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	0	10	theme	polysaccharide	91:104	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	0	10	theme	polysaccharide	91:104	arg1	activity					71:78	in vivo immunoregulatory activity	46:78	in vivo immunoregulatory activity	46:78	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	5	11	theme	water-soluble	871:883	arg1	polysaccharides					885:899	the newly identified water-soluble polysaccharides	850:899	the newly identified water-soluble polysaccharides of PSP3	850:907	According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo.					
32479947	5	12	theme	preliminary	810:820	arg1	results					832:838	the preliminary screening results	806:838	the preliminary screening results in vitro	806:847	According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo.					
32479947	0	13	theme	in	46:47	arg1	activity					71:78	in vivo immunoregulatory activity	46:78	in vivo immunoregulatory activity	46:78	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	3	14	theme	NMR	565:567	arg1	analyses					578:585	NMR spectral analyses	565:585	NMR spectral analyses	565:585	Acid hydrolysis and FT-IR spectral and NMR spectral analyses were employed for structural analysis.					
32479947	4	15	theme	monosaccharide	697:710	arg1	composition					712:722	monosaccharide composition	697:722	monosaccharide composition	697:722	Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro.					
32479947	0	16	from	activity	71:78	arg1	sibiricum					123:131	Polygonatum sibiricum	111:131	Polygonatum sibiricum	111:131	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	4	17	theme	different	731:739	arg1	abilities					741:749	different abilities	731:749	different abilities to activate phagocytic activity in vitro	731:790	Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro.					
32479947	6	18	theme	promising	1056:1064	arg1	candidate					1066:1074	a promising candidate	1054:1074	a promising candidate as an immunomodulator	1054:1096	The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator.					
32479947	6	18	theme	promising	1056:1064	arg1	PSP3					985:988	PSP3	985:988	PSP3	985:988	The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator.					
32479947	3	19	theme	structural	605:614	arg1	analysis					616:623	structural analysis	605:623	structural analysis	605:623	Acid hydrolysis and FT-IR spectral and NMR spectral analyses were employed for structural analysis.					
32479947	5	20	dep	in	840:841	arg1	vitro					843:847	vitro	843:847	vitro	843:847	According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo.					
32479947	4	21	theme	chemical	674:681	arg1	structure					683:691	different chemical structure	664:691	different chemical structure	664:691	Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro.					
32479947	0	22	theme	structural	14:23	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	4	23	theme	different	664:672	arg1	structure					683:691	different chemical structure	664:691	different chemical structure	664:691	Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro.					
32479947	2	24	theme	Flow	492:495	arg1	chromatography					510:523	DEAE-Sepharose Fast Flow ion-exchange chromatography	472:523	DEAE-Sepharose Fast Flow ion-exchange chromatography	472:523	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	1	25	theme	optimal	320:326	arg1	fraction					328:335	the optimal fraction	316:335	the optimal fraction for further development and application	316:375	The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application.					
32479947	2	26	theme	Fast	487:490	arg1	chromatography					510:523	DEAE-Sepharose Fast Flow ion-exchange chromatography	472:523	DEAE-Sepharose Fast Flow ion-exchange chromatography	472:523	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	4	27	with	PSP	655:657	arg1	structure					683:691	different chemical structure	664:691	different chemical structure	664:691	Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro.					
32479947	4	27	with	PSP	655:657	arg1	composition					712:722	monosaccharide composition	697:722	monosaccharide composition	697:722	Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro.					
32479947	5	28	theme	further	927:933	arg1	evaluation					935:944	further evaluation	927:944	further evaluation in vivo	927:952	According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo.					
32479947	2	29	theme	DEAE-Sepharose	472:485	arg1	chromatography					510:523	DEAE-Sepharose Fast Flow ion-exchange chromatography	472:523	DEAE-Sepharose Fast Flow ion-exchange chromatography	472:523	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	1	30	attach	isolated	189:196	arg1	sibiricum					215:223	Polygonatum sibiricum	203:223	Polygonatum sibiricum	203:223	The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application.					
32479947	1	30	attach	isolated	189:196	arg2	polysaccharides					173:187	polysaccharides	173:187	polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application	173:375	The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application.					
32479947	0	31	dep	in	46:47	arg1	vivo					49:52	vivo	49:52	vivo	49:52	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	5	32	theme	identified	860:869	arg1	polysaccharides					885:899	the newly identified water-soluble polysaccharides	850:899	the newly identified water-soluble polysaccharides of PSP3	850:907	According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo.					
32479947	1	33	theme	further	341:347	arg1	development					349:359	further development	341:359	further development	341:359	The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application.					
32479947	2	34	from	sibiricum	445:453	arg1	fractions					399:407	Four polysaccharides fractions	378:407	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum	378:453	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	34	from	sibiricum	445:453	arg1	PSP3					422:425	PSP3	422:425	PSP3	422:425	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	34	from	sibiricum	445:453	arg1	PSP2					416:419	PSP2	416:419	PSP2	416:419	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	34	from	sibiricum	445:453	arg1	PSP4					431:434	PSP4	431:434	PSP4	431:434	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	2	34	from	sibiricum	445:453	arg1	PSP1					410:413	PSP1	410:413	PSP1	410:413	Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography.					
32479947	0	35	from	sibiricum	123:131	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	0	35	from	sibiricum	123:131	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	0	35	from	sibiricum	123:131	arg1	activity					71:78	in vivo immunoregulatory activity	46:78	in vivo immunoregulatory activity	46:78	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	0	35	from	sibiricum	123:131	arg1	polysaccharide					91:104	a novel polysaccharide	83:104	a novel polysaccharide from Polygonatum sibiricum	83:131	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	3	36	theme	Acid	526:529	arg1	hydrolysis					531:540	Acid hydrolysis	526:540	Acid hydrolysis	526:540	Acid hydrolysis and FT-IR spectral and NMR spectral analyses were employed for structural analysis.					
32479947	3	37	theme	spectral	569:576	arg1	analyses					578:585	NMR spectral analyses	565:585	NMR spectral analyses	565:585	Acid hydrolysis and FT-IR spectral and NMR spectral analyses were employed for structural analysis.					
32479947	1	38	theme	structure-activity	242:259	arg1	relationships					261:273	the structure-activity relationships	238:273	the structure-activity relationships of the active substances	238:298	The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application.					
32479947	5	39	theme	screening	822:830	arg1	results					832:838	the preliminary screening results	806:838	the preliminary screening results in vitro	806:847	According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo.					
32479947	3	40	theme	FT-IR	546:550	arg1	spectral					552:559	FT-IR spectral	546:559	FT-IR spectral	546:559	Acid hydrolysis and FT-IR spectral and NMR spectral analyses were employed for structural analysis.					
32479947	6	41	contain	possessed	990:998	arg2	function					1020:1027	an immunomodulatory function	1000:1027	an immunomodulatory function	1000:1027	The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator.					
32479947	6	41	contain	possessed	990:998	arg1	candidate					1066:1074	a promising candidate	1054:1074	a promising candidate as an immunomodulator	1054:1096	The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator.					
32479947	6	41	contain	possessed	990:998	arg1	PSP3					985:988	PSP3	985:988	PSP3	985:988	The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator.					
32479947	0	42	theme	immunoregulatory	54:69	arg1	activity					71:78	in vivo immunoregulatory activity	46:78	in vivo immunoregulatory activity	46:78	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	0	43	from	characterization	25:40	arg1	sibiricum					123:131	Polygonatum sibiricum	111:131	Polygonatum sibiricum	111:131	Purification, structural characterization and in vivo immunoregulatory activity of a novel polysaccharide from Polygonatum sibiricum.					
32479947	5	44	theme	PSP3	904:907	arg1	polysaccharides					885:899	the newly identified water-soluble polysaccharides	850:899	the newly identified water-soluble polysaccharides of PSP3	850:907	According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo.					
32479947	1	45	theme	study	154:158	arg1	purpose					138:144	The purpose	134:144	The purpose of this study	134:158	The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application.					
31968367	7	0	theme	adsorption	1099:1108	arg1	activity					1110:1117	high adsorption activity	1094:1117	high adsorption activity (25.06; 21.35; 20.6 g respectively)	1094:1153	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	0	theme	adsorption	1099:1108	arg1	activity					1172:1179	optimal osmotic activity	1156:1179	optimal osmotic activity (155, 171, 100%)	1156:1196	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	1	1	theme	antiviral	206:214	arg1	properties					243:252	antiviral, adsorptive and protective properties	206:252	antiviral, adsorptive and protective properties	206:252	In many cases the nasal cavity is the gateway for the infection; it necessitates the development of a drug for nasal administration with antiviral, adsorptive and protective properties.					
31968367	4	2	with	METHODS	447:453	arg1	concentrations					484:497	3, 4 and 5% concentrations	472:497	3, 4 and 5% concentrations	472:497	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	6	3	dep	results	847:853	arg1	the					818:820	the	818:820	the	818:820	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	3	dep	results	847:853	arg1	totality					822:829	totality	822:829	totality	822:829	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	4	4	theme	FMC	612:614	arg1	LKХ					607:609	LKХ	607:609	LKХ	607:609	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	4	theme	FMC	612:614	arg1	BioPolymer					616:625	FMC BioPolymer	612:625	FMC BioPolymer	612:625	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	6	5	theme	form	898:901	arg1	development					903:913	the dosage form development	887:913	the dosage form development	887:913	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	7	6	theme	nasal	1297:1301	arg1	cavity					1303:1308	the nasal cavity	1293:1308	the nasal cavity	1293:1308	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	5	7	dep	Adsorption	703:712	arg1	activity					726:733	activity	726:733	activity	726:733	Adsorption and osmotic activity, bioadhesion and thermostability of the samples were determined.					
31968367	1	8	theme	adsorptive	217:226	arg1	properties					243:252	antiviral, adsorptive and protective properties	206:252	antiviral, adsorptive and protective properties	206:252	In many cases the nasal cavity is the gateway for the infection; it necessitates the development of a drug for nasal administration with antiviral, adsorptive and protective properties.					
31968367	7	9	theme	high	1094:1097	arg1	activity					1110:1117	high adsorption activity	1094:1117	high adsorption activity (25.06; 21.35; 20.6 g respectively)	1094:1153	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	9	theme	high	1094:1097	arg1	activity					1172:1179	optimal osmotic activity	1156:1179	optimal osmotic activity (155, 171, 100%)	1156:1196	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	1	10	from	gateway	107:113	arg1	cases					77:81	many cases	72:81	many cases	72:81	In many cases the nasal cavity is the gateway for the infection; it necessitates the development of a drug for nasal administration with antiviral, adsorptive and protective properties.					
31968367	4	11	theme	xanthan	632:638	arg1	agents					541:546	the following gelling agents	519:546	the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco)	519:700	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	11	theme	xanthan	632:638	arg1	gum					640:642	xanthan gum	632:642	xanthan gum	632:642	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	9	12	theme	nasal	1501:1505	arg1	gel					1507:1509	nasal gel	1501:1509	nasal gel with an immunobiological substance	1501:1544	CONCLUSION The obtained data shows prospects of applying the samples for the development of nasal gel with an immunobiological substance.					
31968367	4	13	theme	alginate	556:563	arg1	CR8223					574:579	sodium alginate Protanal CR8223	549:579	sodium alginate Protanal CR8223	549:579	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	13	theme	alginate	556:563	arg1	agents					541:546	the following gelling agents	519:546	the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco)	519:700	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	6	14	theme	%	1050:1050	arg1	concentration					1052:1064	5% concentration	1049:1064	Xanthural 180 5% concentration	1035:1064	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	15	theme	particle	944:951	arg1	gums					938:941	xanthan gums	930:941	xanthan gums (particle size 180pm) from various manufacturers	930:990	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	15	theme	particle	944:951	arg1	size					953:956	particle size 180pm	944:962	particle size 180pm	944:962	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	0	16	theme	Biopolymer	38:47	arg1	Base					49:52	the Biopolymer Base	34:52	the Biopolymer Base for Nasal Gel	34:66	Development of the Composition of the Biopolymer Base for Nasal Gel.					
31968367	8	17	theme	selected	1341:1348	arg1	compositions					1350:1361	All selected compositions	1337:1361	All selected compositions	1337:1361	All selected compositions demonstrated good high-temperature stability.					
31968367	9	18	theme	gel	1507:1509	arg1	development					1486:1496	the development	1482:1496	the development of nasal gel with an immunobiological substance	1482:1544	CONCLUSION The obtained data shows prospects of applying the samples for the development of nasal gel with an immunobiological substance.					
31968367	4	19	theme	gelling	533:539	arg1	CR8223					574:579	sodium alginate Protanal CR8223	549:579	sodium alginate Protanal CR8223	549:579	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	19	theme	gelling	533:539	arg1	agents					541:546	the following gelling agents	519:546	the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco)	519:700	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	19	theme	gelling	533:539	arg1	LKХ					607:609	LKХ	607:609	LKХ	607:609	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	19	theme	gelling	533:539	arg1	gum					640:642	xanthan gum	632:642	xanthan gum	632:642	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	6	20	dep	concentrations	1019:1032	arg1	%					1017:1017	%	1017:1017	%	1017:1017	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	21	theme	various	970:976	arg1	manufacturers					978:990	various manufacturers	970:990	various manufacturers	970:990	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	4	22	theme	%	482:482	arg1	concentrations					484:497	3, 4 and 5% concentrations	472:497	3, 4 and 5% concentrations	472:497	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	5	23	theme	samples	775:781	arg1	thermostability					752:766	thermostability	752:766	thermostability	752:766	Adsorption and osmotic activity, bioadhesion and thermostability of the samples were determined.					
31968367	5	23	theme	samples	775:781	arg1	osmotic					718:724	osmotic	718:724	osmotic	718:724	Adsorption and osmotic activity, bioadhesion and thermostability of the samples were determined.					
31968367	5	23	theme	samples	775:781	arg1	bioadhesion					736:746	bioadhesion	736:746	bioadhesion	736:746	Adsorption and osmotic activity, bioadhesion and thermostability of the samples were determined.					
31968367	5	23	theme	samples	775:781	arg1	Adsorption					703:712	Adsorption	703:712	Adsorption	703:712	Adsorption and osmotic activity, bioadhesion and thermostability of the samples were determined.					
31968367	1	24	theme	protective	232:241	arg1	properties					243:252	antiviral, adsorptive and protective properties	206:252	antiviral, adsorptive and protective properties	206:252	In many cases the nasal cavity is the gateway for the infection; it necessitates the development of a drug for nasal administration with antiviral, adsorptive and protective properties.					
31968367	7	25	theme	long	1316:1319	arg1	period					1321:1326	a long period	1314:1326	a long period of time	1314:1334	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	1	26	theme	many	72:75	arg1	cases					77:81	many cases	72:81	many cases	72:81	In many cases the nasal cavity is the gateway for the infection; it necessitates the development of a drug for nasal administration with antiviral, adsorptive and protective properties.					
31968367	6	27	dep	Xanthural	1035:1043	arg1	concentration					1052:1064	5% concentration	1049:1064	Xanthural 180 5% concentration	1035:1064	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	0	28	theme	Composition	19:29	arg1	Development					0:10	Development	0:10	Development of the Composition of the Biopolymer Base for Nasal Gel.	0:67	Development of the Composition of the Biopolymer Base for Nasal Gel.					
31968367	4	29	dep	MATERIALS	433:441	arg1	samples					459:465	Gel samples	455:465	Gel samples	455:465	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	9	30	theme	immunobiological	1519:1534	arg1	substance					1536:1544	an immunobiological substance	1516:1544	an immunobiological substance	1516:1544	CONCLUSION The obtained data shows prospects of applying the samples for the development of nasal gel with an immunobiological substance.					
31968367	3	31	theme	future	413:418	arg1	form					427:430	the future dosage form	409:430	the future dosage form	409:430	The study is aimed at studying the properties of biopolymer gelling agents and determining the composition of the future dosage form.					
31968367	6	32	from	manufacturers	978:990	arg1	gums					938:941	xanthan gums	930:941	xanthan gums (particle size 180pm) from various manufacturers	930:990	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	32	from	manufacturers	978:990	arg1	size					953:956	particle size 180pm	944:962	particle size 180pm	944:962	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	8	33	theme	high-temperature	1381:1396	arg1	stability					1398:1406	good high-temperature stability	1376:1406	good high-temperature stability	1376:1406	All selected compositions demonstrated good high-temperature stability.					
31968367	7	34	dep	activity	1110:1117	arg1	25.06					1120:1124	25.06	1120:1124	25.06	1120:1124	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	3	35	theme	dosage	420:425	arg1	form					427:430	the future dosage form	409:430	the future dosage form	409:430	The study is aimed at studying the properties of biopolymer gelling agents and determining the composition of the future dosage form.					
31968367	3	36	theme	gelling	359:365	arg1	agents					367:372	biopolymer gelling agents	348:372	biopolymer gelling agents	348:372	The study is aimed at studying the properties of biopolymer gelling agents and determining the composition of the future dosage form.					
31968367	8	37	theme	good	1376:1379	arg1	stability					1398:1406	good high-temperature stability	1376:1406	good high-temperature stability	1376:1406	All selected compositions demonstrated good high-temperature stability.					
31968367	9	38	theme	obtained	1424:1431	arg1	data					1433:1436	The obtained data	1420:1436	The obtained data	1420:1436	CONCLUSION The obtained data shows prospects of applying the samples for the development of nasal gel with an immunobiological substance.					
31968367	7	39	dep	28.4421	1232:1238	arg1	30.2835 N					1250:1258	30.2835 N	1250:1258	30.2835 N	1250:1258	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	39	dep	28.4421	1232:1238	arg1	22.7237					1241:1247	22.7237	1241:1247	22.7237	1241:1247	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	40	theme	osmotic	1164:1170	arg1	activity					1110:1117	high adsorption activity	1094:1117	high adsorption activity (25.06; 21.35; 20.6 g respectively)	1094:1153	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	40	theme	osmotic	1164:1170	arg1	%					1195:1195	155, 171, 100%	1182:1195	155, 171, 100%	1182:1195	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	40	theme	osmotic	1164:1170	arg1	activity					1172:1179	optimal osmotic activity	1156:1179	optimal osmotic activity (155, 171, 100%)	1156:1196	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	3	41	theme	agents	367:372	arg1	properties					334:343	the properties	330:343	the properties of biopolymer gelling agents	330:372	The study is aimed at studying the properties of biopolymer gelling agents and determining the composition of the future dosage form.					
31968367	7	42	dep	25.06	1120:1124	arg1	20.6 g					1134:1139	20.6 g	1134:1139	20.6 g	1134:1139	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	42	dep	25.06	1120:1124	arg1	21.35					1127:1131	21.35	1127:1131	21.35	1127:1131	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	43	theme	time	1331:1334	arg1	period					1321:1326	a long period	1314:1326	a long period of time	1314:1334	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	44	contain	have	1202:1205	arg2	bioadhesion					1219:1229	significant bioadhesion	1207:1229	significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time	1207:1334	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	44	contain	have	1202:1205	arg1	compositions					1073:1084	These compositions	1067:1084	These compositions	1067:1084	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	9	45	with	gel	1507:1509	arg1	substance					1536:1544	an immunobiological substance	1516:1544	an immunobiological substance	1516:1544	CONCLUSION The obtained data shows prospects of applying the samples for the development of nasal gel with an immunobiological substance.					
31968367	1	46	theme	drug	171:174	arg1	development					154:164	the development	150:164	the development of a drug for nasal administration with antiviral, adsorptive and protective properties	150:252	In many cases the nasal cavity is the gateway for the infection; it necessitates the development of a drug for nasal administration with antiviral, adsorptive and protective properties.					
31968367	0	47	theme	Base	49:52	arg1	Composition					19:29	the Composition	15:29	the Composition of the Biopolymer Base for Nasal Gel	15:66	Development of the Composition of the Biopolymer Base for Nasal Gel.					
31968367	9	48	dep	CONCLUSION	1409:1418	arg1	shows					1438:1442	shows	1438:1442	shows prospects of applying the samples for the development of nasal gel with an immunobiological substance	1438:1544	CONCLUSION The obtained data shows prospects of applying the samples for the development of nasal gel with an immunobiological substance.					
31968367	6	49	theme	prospective	871:881	arg1	compositions					858:869	3 compositions	856:869	3 compositions prospective for the dosage form development	856:913	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	7	50	theme	significant	1207:1217	arg1	bioadhesion					1219:1229	significant bioadhesion	1207:1229	significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time	1207:1334	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	1	51	theme	nasal	87:91	arg1	cavity					93:98	the nasal cavity	83:98	the nasal cavity	83:98	In many cases the nasal cavity is the gateway for the infection; it necessitates the development of a drug for nasal administration with antiviral, adsorptive and protective properties.					
31968367	1	51	theme	nasal	87:91	arg1	gateway					107:113	the gateway	103:113	the gateway for the infection	103:131	In many cases the nasal cavity is the gateway for the infection; it necessitates the development of a drug for nasal administration with antiviral, adsorptive and protective properties.					
31968367	3	52	theme	biopolymer	348:357	arg1	agents					367:372	biopolymer gelling agents	348:372	biopolymer gelling agents	348:372	The study is aimed at studying the properties of biopolymer gelling agents and determining the composition of the future dosage form.					
31968367	6	53	theme	obtained	838:845	arg1	results					847:853	the obtained results	834:853	the obtained results	834:853	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	3	54	theme	form	427:430	arg1	composition					394:404	the composition	390:404	the composition of the future dosage form	390:430	The study is aimed at studying the properties of biopolymer gelling agents and determining the composition of the future dosage form.					
31968367	6	55	theme	dosage	891:896	arg1	development					903:913	the dosage form development	887:913	the dosage form development	887:913	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	7	56	theme	optimal	1156:1162	arg1	activity					1110:1117	high adsorption activity	1094:1117	high adsorption activity (25.06; 21.35; 20.6 g respectively)	1094:1153	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	56	theme	optimal	1156:1162	arg1	%					1195:1195	155, 171, 100%	1182:1195	155, 171, 100%	1182:1195	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	56	theme	optimal	1156:1162	arg1	activity					1172:1179	optimal osmotic activity	1156:1179	optimal osmotic activity (155, 171, 100%)	1156:1196	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	57	contain	possess	1086:1092	arg2	activity					1110:1117	high adsorption activity	1094:1117	high adsorption activity (25.06; 21.35; 20.6 g respectively)	1094:1153	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	57	contain	possess	1086:1092	arg1	compositions					1073:1084	These compositions	1067:1084	These compositions	1067:1084	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	7	57	contain	possess	1086:1092	arg2	activity					1172:1179	optimal osmotic activity	1156:1179	optimal osmotic activity (155, 171, 100%)	1156:1196	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	2	58	theme	dosage	286:291	arg1	form					293:296	an optimal dosage form	275:296	an optimal dosage form	275:296	Gels were chosen as an optimal dosage form.					
31968367	2	58	theme	dosage	286:291	arg1	Gels					255:258	Gels	255:258	Gels	255:258	Gels were chosen as an optimal dosage form.					
31968367	6	59	theme	xanthan	930:936	arg1	gums					938:941	xanthan gums	930:941	xanthan gums (particle size 180pm) from various manufacturers	930:990	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	59	theme	xanthan	930:936	arg1	size					953:956	particle size 180pm	944:962	particle size 180pm	944:962	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	60	theme	5	1049:1049	arg1	%					1050:1050	%	1050:1050	%	1050:1050	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	4	61	dep	agents	541:546	arg1	gum					640:642	xanthan gum	632:642	xanthan gum	632:642	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	61	dep	agents	541:546	arg1	CR8223					574:579	sodium alginate Protanal CR8223	549:579	sodium alginate Protanal CR8223	549:579	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	61	dep	agents	541:546	arg1	Xanthural					677:685	Xanthural	677:685	Xanthural	677:685	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	61	dep	agents	541:546	arg1	LKХ					607:609	LKХ	607:609	LKХ	607:609	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	61	dep	agents	541:546	arg1	agents					541:546	the following gelling agents	519:546	the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco)	519:700	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	61	dep	agents	541:546	arg1	GrinstedXanthan					644:658	GrinstedXanthan	644:658	GrinstedXanthan	644:658	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	61	dep	agents	541:546	arg1	BioPolymer					616:625	FMC BioPolymer	612:625	FMC BioPolymer	612:625	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	61	dep	agents	541:546	arg1	DuPontDanisco					661:673	DuPontDanisco	661:673	DuPontDanisco	661:673	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	61	dep	agents	541:546	arg1	Manucol					599:605	Manucol	599:605	Manucol	599:605	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	62	theme	CP	692:693	arg1	Xanthural					677:685	Xanthural	677:685	Xanthural	677:685	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	62	theme	CP	692:693	arg1	Kelco					695:699	CP Kelco	692:699	CP Kelco	692:699	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	1	63	with	administration	186:199	arg1	properties					243:252	antiviral, adsorptive and protective properties	206:252	antiviral, adsorptive and protective properties	206:252	In many cases the nasal cavity is the gateway for the infection; it necessitates the development of a drug for nasal administration with antiviral, adsorptive and protective properties.					
31968367	2	64	theme	optimal	278:284	arg1	form					293:296	an optimal dosage form	275:296	an optimal dosage form	275:296	Gels were chosen as an optimal dosage form.					
31968367	2	64	theme	optimal	278:284	arg1	Gels					255:258	Gels	255:258	Gels	255:258	Gels were chosen as an optimal dosage form.					
31968367	1	65	theme	nasal	180:184	arg1	administration					186:199	nasal administration	180:199	nasal administration with antiviral, adsorptive and protective properties	180:252	In many cases the nasal cavity is the gateway for the infection; it necessitates the development of a drug for nasal administration with antiviral, adsorptive and protective properties.					
31968367	0	66	theme	Nasal	58:62	arg1	Gel					64:66	Nasal Gel	58:66	Nasal Gel	58:66	Development of the Composition of the Biopolymer Base for Nasal Gel.					
31968367	6	67	theme	Grinsted	993:1000	arg1	Xanthural					1035:1043	Xanthural	1035:1043	Xanthural	1035:1043	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	67	theme	Grinsted	993:1000	arg1	concentrations					1019:1032	Grinsted Xanthan 3 and 4% concentrations	993:1032	Grinsted Xanthan 3 and 4% concentrations	993:1032	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	68	dep	%	1017:1017	arg1	4					1016:1016	4	1016:1016	4	1016:1016	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	68	dep	%	1017:1017	arg1	3					1010:1010	3	1010:1010	3	1010:1010	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	4	69	theme	Protanal	565:572	arg1	CR8223					574:579	sodium alginate Protanal CR8223	549:579	sodium alginate Protanal CR8223	549:579	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	69	theme	Protanal	565:572	arg1	agents					541:546	the following gelling agents	519:546	the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco)	519:700	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	6	70	theme	Xanthan	1002:1008	arg1	Xanthural					1035:1043	Xanthural	1035:1043	Xanthural	1035:1043	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	6	70	theme	Xanthan	1002:1008	arg1	concentrations					1019:1032	Grinsted Xanthan 3 and 4% concentrations	993:1032	Grinsted Xanthan 3 and 4% concentrations	993:1032	RESULTS Basing on the totality of the obtained results, 3 compositions prospective for the dosage form development were selected: xanthan gums (particle size 180pm) from various manufacturers: Grinsted Xanthan 3 and 4% concentrations, Xanthural 180 5% concentration.					
31968367	7	71	dep	bioadhesion	1219:1229	arg1	28.4421					1232:1238	28.4421	1232:1238	28.4421; 22.7237; 30.2835 N	1232:1258	These compositions possess high adsorption activity (25.06; 21.35; 20.6 g respectively), optimal osmotic activity (155, 171, 100%) and have significant bioadhesion (28.4421; 22.7237; 30.2835 N) that allows the drug to stay in the nasal cavity for a long period of time.					
31968367	4	72	theme	sodium	549:554	arg1	CR8223					574:579	sodium alginate Protanal CR8223	549:579	sodium alginate Protanal CR8223	549:579	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	72	theme	sodium	549:554	arg1	agents					541:546	the following gelling agents	519:546	the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco)	519:700	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	73	theme	following	523:531	arg1	CR8223					574:579	sodium alginate Protanal CR8223	549:579	sodium alginate Protanal CR8223	549:579	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	73	theme	following	523:531	arg1	agents					541:546	the following gelling agents	519:546	the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco)	519:700	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	73	theme	following	523:531	arg1	LKХ					607:609	LKХ	607:609	LKХ	607:609	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	73	theme	following	523:531	arg1	gum					640:642	xanthan gum	632:642	xanthan gum	632:642	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	74	theme	Gel	455:457	arg1	samples					459:465	Gel samples	455:465	Gel samples	455:465	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
31968367	4	75	with	MATERIALS	433:441	arg1	concentrations					484:497	3, 4 and 5% concentrations	472:497	3, 4 and 5% concentrations	472:497	MATERIALS AND METHODS Gel samples with 3, 4 and 5% concentrations were prepared using the following gelling agents: sodium alginate Protanal CR8223, Protanal CR8133, Manucol LKХ (FMC BioPolymer) and xanthan gum GrinstedXanthan (DuPontDanisco), Xanthural 180 (CP Kelco).					
33097931	6	0	theme	milk	1102:1105	arg1	replacer					1107:1114	their experimental milk replacer	1083:1114	their experimental milk replacer in 2 meals	1083:1125	Between 7 and 9 wk of age, groups were housed for 72 h in climate respiration chambers, and fed their experimental milk replacer in 2 meals per day, at 08:30 and 16:30.					
33097931	2	1	theme	energy	344:349	arg1	metabolism					351:360	energy metabolism	344:360	energy metabolism	344:360	Digestible maltodextrins are commonly used as a substitute for lactose in these formulas, but the effects on energy metabolism are unknown.					
33097931	11	2	theme	resting	1887:1893	arg1	rate					1905:1908	resting metabolic rate	1887:1908	resting metabolic rate in the fasting state	1887:1929	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	7	3	theme	exchanges	1236:1244	arg1	measurement					1213:1223	the continuous measurement	1198:1223	the continuous measurement of gaseous exchanges	1198:1244	Heat production data were calculated from the continuous measurement of gaseous exchanges and analyzed using general linear models in SAS.					
33097931	10	4	theme	carbohydrate	1547:1558	arg1	oxidation					1560:1568	carbohydrate oxidation	1547:1568	carbohydrate oxidation	1547:1568	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	7	5	theme	linear	1273:1278	arg1	models					1280:1285	general linear models	1265:1285	general linear models in SAS	1265:1292	Heat production data were calculated from the continuous measurement of gaseous exchanges and analyzed using general linear models in SAS.					
33097931	2	6	from	effects	333:339	arg1	metabolism					351:360	energy metabolism	344:360	energy metabolism	344:360	Digestible maltodextrins are commonly used as a substitute for lactose in these formulas, but the effects on energy metabolism are unknown.					
33097931	11	7	from	rate	1905:1908	arg1	state					1925:1929	the fasting state	1913:1929	the fasting state	1913:1929	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	6	8	theme	9	1001:1001	arg1	wk					1003:1004	7 and 9 wk	995:1004	wk	1003:1004	Between 7 and 9 wk of age, groups were housed for 72 h in climate respiration chambers, and fed their experimental milk replacer in 2 meals per day, at 08:30 and 16:30.					
33097931	1	9	theme	infant	190:195	arg1	formulas					197:204	lactose-free infant formulas	177:204	lactose-free infant formulas	177:204	BACKGROUND In recent years, lactose-free infant formulas have been increasingly used.					
33097931	6	10	theme	7	995:995	arg1	wk					1003:1004	7 and 9 wk	995:1004	wk	1003:1004	Between 7 and 9 wk of age, groups were housed for 72 h in climate respiration chambers, and fed their experimental milk replacer in 2 meals per day, at 08:30 and 16:30.					
33097931	0	11	dep	Metabolism	80:89	arg1	Study					131:135	A Calorimetric Study	116:135	Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets	73:146	Lactose and Digestible Maltodextrin in Milk Replacers Differently Affect Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets.					
33097931	0	12	theme	Calorimetric	118:129	arg1	Study					131:135	A Calorimetric Study	116:135	Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets	73:146	Lactose and Digestible Maltodextrin in Milk Replacers Differently Affect Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets.					
33097931	3	13	theme	carbohydrates	565:577	arg1	source					555:560	the only source	546:560	the only source of carbohydrates	546:577	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	3	13	theme	carbohydrates	565:577	arg1	lactose					508:514	lactose	508:514	lactose compared with maltodextrin	508:541	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	7	14	from	models	1280:1285	arg1	SAS					1290:1292	SAS	1290:1292	SAS	1290:1292	Heat production data were calculated from the continuous measurement of gaseous exchanges and analyzed using general linear models in SAS.					
33097931	6	15	theme	respiration	1053:1063	arg1	chambers					1065:1072	climate respiration chambers	1045:1072	climate respiration chambers	1045:1072	Between 7 and 9 wk of age, groups were housed for 72 h in climate respiration chambers, and fed their experimental milk replacer in 2 meals per day, at 08:30 and 16:30.					
33097931	2	16	theme	Digestible	235:244	arg1	a					281:281	a	281:281	a	281:281	Digestible maltodextrins are commonly used as a substitute for lactose in these formulas, but the effects on energy metabolism are unknown.					
33097931	2	16	theme	Digestible	235:244	arg1	maltodextrins					246:258	Digestible maltodextrins	235:258	Digestible maltodextrins	235:258	Digestible maltodextrins are commonly used as a substitute for lactose in these formulas, but the effects on energy metabolism are unknown.					
33097931	5	17	theme	age	783:785	arg1	wk					777:778	5 wk	775:778	5 wk of age	775:785	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	7	18	theme	production	1161:1170	arg1	data					1172:1175	Heat production data	1156:1175	Heat production data	1156:1175	Heat production data were calculated from the continuous measurement of gaseous exchanges and analyzed using general linear models in SAS.					
33097931	4	19	theme	milk	700:703	arg1	%					693:693	28% w/w	691:697	28% w/w	691:697	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	4	19	theme	milk	700:703	arg1	basis					712:716	milk powder basis	700:716	milk powder basis	700:716	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	10	20	theme	lactose-fed	1755:1765	arg1	piglets					1767:1773	lactose-fed piglets	1755:1773	lactose-fed piglets	1755:1773	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	5	21	theme	littermates	909:919	arg1	groups					897:902	16 groups	894:902	16 groups of 4 littermates	894:919	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	21	theme	littermates	909:919	arg1	littermates					909:919	4 littermates	907:919	4 littermates	907:919	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	22	theme	±	823:823	arg1	0.3 kg					846:851	10 ± 0.3 kg	841:851	10 ± 0.3 kg	841:851	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	22	theme	±	823:823	arg1	bodyweight					829:838	mean ± SEM bodyweight	818:838	mean ± SEM bodyweight	818:838	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	10	23	theme	morning	1799:1805	arg1	meal					1807:1810	the morning meal	1795:1810	the morning meal	1795:1810	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	10	24	theme	oxidation	1560:1568	arg1	rates					1538:1542	Net rates	1534:1542	Net rates of carbohydrate oxidation	1534:1568	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	5	25	theme	groups/milk	964:974	arg1	replacer					976:983	n = 8 groups/milk replacer	958:983	n = 8 groups/milk replacer	958:983	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	25	theme	groups/milk	964:974	arg1	pen					953:955	pen	953:955	pen (n = 8 groups/milk replacer)	953:984	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	26	theme	entire	804:809	arg1	males					811:815	4 entire males	802:815	4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg)	802:852	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	8	27	theme	RESULTS	1295:1301	arg1	rate					1321:1324	RESULTS Resting metabolic rate	1295:1324	RESULTS Resting metabolic rate	1295:1324	RESULTS Resting metabolic rate was 6% less in maltodextrin- than in lactose-fed piglets, notably before the morning meal.					
33097931	1	28	theme	recent	163:168	arg1	years					170:174	recent years	163:174	recent years	163:174	BACKGROUND In recent years, lactose-free infant formulas have been increasingly used.					
33097931	8	29	theme	metabolic	1311:1319	arg1	rate					1321:1324	RESULTS Resting metabolic rate	1295:1324	RESULTS Resting metabolic rate	1295:1324	RESULTS Resting metabolic rate was 6% less in maltodextrin- than in lactose-fed piglets, notably before the morning meal.					
33097931	0	30	theme	Substrate	95:103	arg1	Oxidation					105:113	Substrate Oxidation	95:113	Substrate Oxidation	95:113	Lactose and Digestible Maltodextrin in Milk Replacers Differently Affect Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets.					
33097931	10	31	dep	average	1578:1584	arg1	whereas					1682:1688	whereas	1682:1688	whereas	1682:1688	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	4	32	theme	age	737:739	arg1	wk					731:732	1 to 9 wk	724:732	1 to 9 wk of age (n = 4 litters/milk replacer)	724:769	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	0	33	theme	Energy	73:78	arg1	Metabolism					80:89	Energy Metabolism	73:89	Energy Metabolism	73:89	Lactose and Digestible Maltodextrin in Milk Replacers Differently Affect Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets.					
33097931	5	34	dep	males	811:815	arg1	0.3 kg					846:851	10 ± 0.3 kg	841:851	10 ± 0.3 kg	841:851	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	34	dep	males	811:815	arg1	bodyweight					829:838	mean ± SEM bodyweight	818:838	mean ± SEM bodyweight	818:838	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	35	theme	10 ±	841:844	arg1	0.3 kg					846:851	10 ± 0.3 kg	841:851	10 ± 0.3 kg	841:851	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	35	theme	10 ±	841:844	arg1	bodyweight					829:838	mean ± SEM bodyweight	818:838	mean ± SEM bodyweight	818:838	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	0	36	theme	Digestible	12:21	arg1	Maltodextrin					23:34	Digestible Maltodextrin	12:34	Digestible Maltodextrin in Milk Replacers	12:52	Lactose and Digestible Maltodextrin in Milk Replacers Differently Affect Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets.					
33097931	11	37	theme	substrate	1968:1976	arg1	profiles					1988:1995	postprandial substrate oxidation profiles	1955:1995	postprandial substrate oxidation profiles in pigs	1955:2003	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	3	38	theme	substrate	447:455	arg1	oxidation					457:465	substrate oxidation	447:465	substrate oxidation	447:465	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	8	39	theme	lactose-fed	1363:1373	arg1	piglets					1375:1381	lactose-fed piglets	1363:1381	lactose-fed piglets	1363:1381	RESULTS Resting metabolic rate was 6% less in maltodextrin- than in lactose-fed piglets, notably before the morning meal.					
33097931	0	40	from	Maltodextrin	23:34	arg1	Replacers					44:52	Milk Replacers	39:52	Milk Replacers	39:52	Lactose and Digestible Maltodextrin in Milk Replacers Differently Affect Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets.					
33097931	4	41	theme	n =	742:744	arg1	replacer					761:768	n = 4 litters/milk replacer	742:768	n = 4 litters/milk replacer	742:768	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	4	41	theme	n =	742:744	arg1	age					737:739	age	737:739	age (n = 4 litters/milk replacer)	737:769	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	10	42	theme	afternoon	1666:1674	arg1	meal					1676:1679	the afternoon meal	1662:1679	the afternoon meal	1662:1679	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	4	43	theme	litters/milk	748:759	arg1	replacer					761:768	n = 4 litters/milk replacer	742:768	n = 4 litters/milk replacer	742:768	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	4	43	theme	litters/milk	748:759	arg1	age					737:739	age	737:739	age (n = 4 litters/milk replacer)	737:769	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	10	44	theme	Net	1534:1536	arg1	rates					1538:1542	Net rates	1534:1542	Net rates of carbohydrate oxidation	1534:1568	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	6	45	from	replacer	1107:1114	arg1	meals					1121:1125	2 meals	1119:1125	2 meals	1119:1125	Between 7 and 9 wk of age, groups were housed for 72 h in climate respiration chambers, and fed their experimental milk replacer in 2 meals per day, at 08:30 and 16:30.					
33097931	9	46	theme	respiratory	1434:1444	arg1	quotient					1446:1453	The postprandial respiratory quotient	1417:1453	The postprandial respiratory quotient	1417:1453	The postprandial respiratory quotient was 13% greater in maltodextrin- than in lactose-fed piglets after both meals.					
33097931	9	46	theme	respiratory	1434:1444	arg1	greater					1463:1469	greater	1463:1469	greater	1463:1469	The postprandial respiratory quotient was 13% greater in maltodextrin- than in lactose-fed piglets after both meals.					
33097931	10	47	theme	lactose-fed	1622:1632	arg1	piglets					1634:1640	lactose-fed piglets	1622:1640	lactose-fed piglets	1622:1640	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	11	48	from	shift	1946:1950	arg1	profiles					1988:1995	postprandial substrate oxidation profiles	1955:1995	postprandial substrate oxidation profiles in pigs	1955:2003	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	10	49	theme	net	1690:1692	arg1	rates					1694:1698	net rates	1690:1698	net rates of fat oxidation	1690:1715	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	6	50	theme	experimental	1089:1100	arg1	replacer					1107:1114	their experimental milk replacer	1083:1114	their experimental milk replacer in 2 meals	1083:1125	Between 7 and 9 wk of age, groups were housed for 72 h in climate respiration chambers, and fed their experimental milk replacer in 2 meals per day, at 08:30 and 16:30.					
33097931	3	51	theme	only	550:553	arg1	source					555:560	the only source	546:560	the only source of carbohydrates	546:577	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	3	51	theme	only	550:553	arg1	lactose					508:514	lactose	508:514	lactose compared with maltodextrin	508:541	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	7	52	theme	continuous	1202:1211	arg1	measurement					1213:1223	the continuous measurement	1198:1223	the continuous measurement of gaseous exchanges	1198:1244	Heat production data were calculated from the continuous measurement of gaseous exchanges and analyzed using general linear models in SAS.					
33097931	7	53	theme	general	1265:1271	arg1	models					1280:1285	general linear models	1265:1285	general linear models in SAS	1265:1292	Heat production data were calculated from the continuous measurement of gaseous exchanges and analyzed using general linear models in SAS.					
33097931	11	54	theme	metabolic	1895:1903	arg1	rate					1905:1908	resting metabolic rate	1887:1908	resting metabolic rate in the fasting state	1887:1929	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	6	55	theme	age	1009:1011	arg1	wk					1003:1004	7 and 9 wk	995:1004	wk	1003:1004	Between 7 and 9 wk of age, groups were housed for 72 h in climate respiration chambers, and fed their experimental milk replacer in 2 meals per day, at 08:30 and 16:30.					
33097931	7	56	theme	gaseous	1228:1234	arg1	exchanges					1236:1244	gaseous exchanges	1228:1244	gaseous exchanges	1228:1244	Heat production data were calculated from the continuous measurement of gaseous exchanges and analyzed using general linear models in SAS.					
33097931	2	57	used	used	273:276	arg2	a					281:281	a	281:281	a	281:281	Digestible maltodextrins are commonly used as a substitute for lactose in these formulas, but the effects on energy metabolism are unknown.					
33097931	2	57	used	used	273:276	arg2	maltodextrins					246:258	Digestible maltodextrins	235:258	Digestible maltodextrins	235:258	Digestible maltodextrins are commonly used as a substitute for lactose in these formulas, but the effects on energy metabolism are unknown.					
33097931	10	58	theme	oxidation	1707:1715	arg1	rates					1694:1698	net rates	1690:1698	net rates of fat oxidation	1690:1715	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	1	59	theme	lactose-free	177:188	arg1	formulas					197:204	lactose-free infant formulas	177:204	lactose-free infant formulas	177:204	BACKGROUND In recent years, lactose-free infant formulas have been increasingly used.					
33097931	11	60	theme	fasting	1917:1923	arg1	state					1925:1929	the fasting state	1913:1929	the fasting state	1913:1929	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	9	61	theme	lactose-fed	1496:1506	arg1	piglets					1508:1514	lactose-fed piglets	1496:1514	lactose-fed piglets after both meals	1496:1531	The postprandial respiratory quotient was 13% greater in maltodextrin- than in lactose-fed piglets after both meals.					
33097931	12	62	theme	metabolic	2074:2082	arg1	changes					2084:2090	these metabolic changes	2068:2090	these metabolic changes	2068:2090	Further research is warranted to evaluate the consequences of these metabolic changes for body composition.					
33097931	6	63	theme	climate	1045:1051	arg1	chambers					1065:1072	climate respiration chambers	1045:1072	climate respiration chambers	1045:1072	Between 7 and 9 wk of age, groups were housed for 72 h in climate respiration chambers, and fed their experimental milk replacer in 2 meals per day, at 08:30 and 16:30.					
33097931	4	64	theme	milk	640:643	arg1	replacers					645:653	milk replacers	640:653	milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer)	640:769	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	10	65	theme	greater	1589:1595	arg1	average					1578:1584	average	1578:1584	average 5% greater in maltodextrin- than in lactose-fed piglets	1578:1640	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	5	66	theme	mean	818:821	arg1	0.3 kg					846:851	10 ± 0.3 kg	841:851	10 ± 0.3 kg	841:851	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	66	theme	mean	818:821	arg1	bodyweight					829:838	mean ± SEM bodyweight	818:838	mean ± SEM bodyweight	818:838	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	0	67	from	Lactose	0:6	arg1	Replacers					44:52	Milk Replacers	39:52	Milk Replacers	39:52	Lactose and Digestible Maltodextrin in Milk Replacers Differently Affect Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets.					
33097931	12	68	theme	body	2096:2099	arg1	composition					2101:2111	body composition	2096:2111	body composition	2096:2111	Further research is warranted to evaluate the consequences of these metabolic changes for body composition.					
33097931	5	69	theme	SEM	825:827	arg1	0.3 kg					846:851	10 ± 0.3 kg	841:851	10 ± 0.3 kg	841:851	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	69	theme	SEM	825:827	arg1	bodyweight					829:838	mean ± SEM bodyweight	818:838	mean ± SEM bodyweight	818:838	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	11	70	theme	milk	1864:1867	arg1	replacers					1869:1877	milk replacers	1864:1877	milk replacers	1864:1877	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	7	71	theme	Heat	1156:1159	arg1	data					1172:1175	Heat production data	1156:1175	Heat production data	1156:1175	Heat production data were calculated from the continuous measurement of gaseous exchanges and analyzed using general linear models in SAS.					
33097931	4	72	theme	powder	705:710	arg1	%					693:693	28% w/w	691:697	28% w/w	691:697	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	4	72	theme	powder	705:710	arg1	basis					712:716	milk powder basis	700:716	milk powder basis	700:716	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	4	73	from	litters	623:629	arg1	Tempo × Topigs					597:610	Tempo × Topigs	597:610	Tempo × Topigs	597:610	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	4	73	from	litters	623:629	arg1	Piglets					588:594	METHODS Piglets	580:594	METHODS Piglets (Tempo × Topigs 20) from 8 litters	580:629	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	8	74	theme	Resting	1303:1309	arg1	rate					1321:1324	RESULTS Resting metabolic rate	1295:1324	RESULTS Resting metabolic rate	1295:1324	RESULTS Resting metabolic rate was 6% less in maltodextrin- than in lactose-fed piglets, notably before the morning meal.					
33097931	2	75	dep	a	281:281	arg1	substitute					283:292	substitute	283:292	substitute for lactose in these formulas	283:322	Digestible maltodextrins are commonly used as a substitute for lactose in these formulas, but the effects on energy metabolism are unknown.					
33097931	4	76	dep	wk	731:732	arg1	to					726:727	to	726:727	to	726:727	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	12	77	theme	changes	2084:2090	arg1	consequences					2052:2063	the consequences	2048:2063	the consequences of these metabolic changes for body composition	2048:2111	Further research is warranted to evaluate the consequences of these metabolic changes for body composition.					
33097931	3	78	dep	OBJECTIVE	375:383	arg1	aimed					388:392	aimed	388:392	aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates	388:577	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	3	79	from	differences	410:420	arg1	metabolism					432:441	energy metabolism	425:441	energy metabolism	425:441	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	3	79	from	differences	410:420	arg1	oxidation					457:465	substrate oxidation	447:465	substrate oxidation	447:465	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	3	79	from	differences	410:420	arg1	piglets					470:476	piglets	470:476	piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates	470:577	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	12	80	theme	Further	2006:2012	arg1	research					2014:2021	Further research	2006:2021	Further research	2006:2021	Further research is warranted to evaluate the consequences of these metabolic changes for body composition.					
33097931	11	81	from	maltodextrin	1848:1859	arg1	replacers					1869:1877	milk replacers	1864:1877	milk replacers	1864:1877	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	3	82	contain	containing	497:506	arg1	replacers					487:495	milk replacers	482:495	milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates	482:577	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	3	82	contain	containing	497:506	arg2	source					555:560	the only source	546:560	the only source of carbohydrates	546:577	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	3	82	contain	containing	497:506	arg2	lactose					508:514	lactose	508:514	lactose compared with maltodextrin	508:541	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	5	83	theme	n = 8	958:962	arg1	replacer					976:983	n = 8 groups/milk replacer	958:983	n = 8 groups/milk replacer	958:983	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	5	83	theme	n = 8	958:962	arg1	pen					953:955	pen	953:955	pen (n = 8 groups/milk replacer)	953:984	At 5 wk of age, 4 females and 4 entire males (mean ± SEM bodyweight, 10 ± 0.3 kg) were selected per litter, and housed in 16 groups of 4 littermates, with 2 females and 2 males per pen (n = 8 groups/milk replacer).					
33097931	3	84	theme	energy	425:430	arg1	metabolism					432:441	energy metabolism	425:441	energy metabolism	425:441	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	9	85	theme	postprandial	1421:1432	arg1	quotient					1446:1453	The postprandial respiratory quotient	1417:1453	The postprandial respiratory quotient	1417:1453	The postprandial respiratory quotient was 13% greater in maltodextrin- than in lactose-fed piglets after both meals.					
33097931	9	85	theme	postprandial	1421:1432	arg1	greater					1463:1469	greater	1463:1469	greater	1463:1469	The postprandial respiratory quotient was 13% greater in maltodextrin- than in lactose-fed piglets after both meals.					
33097931	4	86	theme	METHODS	580:586	arg1	Tempo × Topigs					597:610	Tempo × Topigs	597:610	Tempo × Topigs	597:610	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	4	86	theme	METHODS	580:586	arg1	Piglets					588:594	METHODS Piglets	580:594	METHODS Piglets (Tempo × Topigs 20) from 8 litters	580:629	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	1	87	dep	BACKGROUND	149:158	arg1	used					229:232	used	229:232	have been increasingly used	206:232	BACKGROUND In recent years, lactose-free infant formulas have been increasingly used.					
33097931	0	88	theme	Milk	39:42	arg1	Replacers					44:52	Milk Replacers	39:52	Milk Replacers	39:52	Lactose and Digestible Maltodextrin in Milk Replacers Differently Affect Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets.					
33097931	11	89	theme	oxidation	1978:1986	arg1	profiles					1988:1995	postprandial substrate oxidation profiles	1955:1995	postprandial substrate oxidation profiles in pigs	1955:2003	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	11	90	theme	postprandial	1955:1966	arg1	profiles					1988:1995	postprandial substrate oxidation profiles	1955:1995	postprandial substrate oxidation profiles in pigs	1955:2003	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	11	91	from	profiles	1988:1995	arg1	pigs					2000:2003	pigs	2000:2003	pigs	2000:2003	CONCLUSIONS Compared with lactose, maltodextrin in milk replacers reduced resting metabolic rate in the fasting state, and induced a shift in postprandial substrate oxidation profiles in pigs.					
33097931	4	92	contain	containing	655:664	arg2	maltodextrin					677:688	maltodextrin	677:688	maltodextrin	677:688	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	4	92	contain	containing	655:664	arg2	lactose					666:672	lactose	666:672	lactose	666:672	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	4	92	contain	containing	655:664	arg1	replacers					645:653	milk replacers	640:653	milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer)	640:769	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	3	93	theme	milk	482:485	arg1	replacers					487:495	milk replacers	482:495	milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates	482:577	OBJECTIVE We aimed to evaluate the differences in energy metabolism and substrate oxidation in piglets fed milk replacers containing lactose compared with maltodextrin as the only source of carbohydrates.					
33097931	1	94	used	used	229:232	arg2	formulas					197:204	lactose-free infant formulas	177:204	lactose-free infant formulas	177:204	BACKGROUND In recent years, lactose-free infant formulas have been increasingly used.					
33097931	4	95	dep	lactose	666:672	arg1	%					693:693	28% w/w	691:697	28% w/w	691:697	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	4	95	dep	lactose	666:672	arg1	basis					712:716	milk powder basis	700:716	milk powder basis	700:716	METHODS Piglets (Tempo × Topigs 20) from 8 litters were fed milk replacers containing lactose or maltodextrin (28% w/w, milk powder basis) from 1 to 9 wk of age (n = 4 litters/milk replacer).					
33097931	0	96	from	Study	131:135	arg1	Piglets					140:146	Piglets	140:146	Piglets	140:146	Lactose and Digestible Maltodextrin in Milk Replacers Differently Affect Energy Metabolism and Substrate Oxidation: A Calorimetric Study in Piglets.					
33097931	10	97	theme	fat	1703:1705	arg1	oxidation					1707:1715	fat oxidation	1703:1715	fat oxidation	1703:1715	Net rates of carbohydrate oxidation were on average 5% greater in maltodextrin- than in lactose-fed piglets, particularly after the afternoon meal, whereas net rates of fat oxidation were 9% less in maltodextrin- than in lactose-fed piglets, particularly after the morning meal.					
33097931	8	98	theme	morning	1403:1409	arg1	meal					1411:1414	the morning meal	1399:1414	the morning meal	1399:1414	RESULTS Resting metabolic rate was 6% less in maltodextrin- than in lactose-fed piglets, notably before the morning meal.					
33142599	1	0	from	farmed	259:264	arg1	China					269:273	China	269:273	China	269:273	Oudemansiella radicata is a commercialized and nutrient-rich mushroom farmed in China.					
33142599	4	1	theme	simulated	599:607	arg1	digestion					609:617	simulated digestion	599:617	simulated digestion	599:617	Results showed that the molecular weight of ORP decreased after simulated digestion.					
33142599	0	2	theme	polysaccharides	92:106	arg1	digestion					33:41	In vitro saliva-gastrointestinal digestion	0:41	In vitro saliva-gastrointestinal digestion	0:41	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	0	2	theme	polysaccharides	92:106	arg1	fermentation					53:64	fecal fermentation	47:64	fecal fermentation of Oudemansiella radicata polysaccharides	47:106	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	3	3	from	effects	424:430	arg1	composition					450:460	gut microbiota composition	435:460	gut microbiota composition	435:460	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	7	4	theme	main	962:965	arg1	acid					913:916	acetic acid	906:916	acetic acid	906:916	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	4	theme	main	962:965	arg1	acid					929:932	propionic acid	919:932	propionic acid	919:932	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	4	theme	main	962:965	arg1	acid					948:951	n-butyric acid	938:951	n-butyric acid	938:951	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	4	theme	main	962:965	arg1	products					967:974	the main products	958:974	the main products	958:974	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	0	5	theme	digestion	119:127	arg1	profile					129:135	its digestion profile	115:135	its digestion profile	115:135	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	6	6	theme	ORP	738:740	arg1	structure					725:733	the overall structure	713:733	the overall structure of ORP	713:740	Besides, the overall structure of ORP was not damaged after digestion.					
33142599	7	7	theme	short-chain	869:879	arg1	acids					887:891	several short-chain fatty acids	861:891	several short-chain fatty acids	861:891	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	3	8	from	fermentation	396:407	arg1	composition					450:460	gut microbiota composition	435:460	gut microbiota composition	435:460	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	8	9	theme	relative	1055:1062	arg1	abundances					1064:1073	the relative abundances	1051:1073	the relative abundances of Bacteroides and Parabacteroides	1051:1108	Notably, ORP could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Parabacteroides.					
33142599	3	10	theme	simulated	481:489	arg1	digestion					491:499	a simulated digestion	479:499	a simulated digestion	479:499	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	7	11	theme	fatty	881:885	arg1	acids					887:891	several short-chain fatty acids	861:891	several short-chain fatty acids	861:891	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	12	theme	n-butyric	938:946	arg1	acid					913:916	acetic acid	906:916	acetic acid	906:916	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	12	theme	n-butyric	938:946	arg1	acid					929:932	propionic acid	919:932	propionic acid	919:932	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	12	theme	n-butyric	938:946	arg1	acid					948:951	n-butyric acid	938:951	n-butyric acid	938:951	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	12	theme	n-butyric	938:946	arg1	products					967:974	the main products	958:974	the main products	958:974	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	3	13	theme	ORP	412:414	arg1	effects					424:430	its effects	420:430	its effects on gut microbiota composition	420:460	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	3	13	theme	ORP	412:414	arg1	fermentation					396:407	fermentation	396:407	fermentation	396:407	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	3	13	theme	ORP	412:414	arg1	digestibility					378:390	digestibility	378:390	digestibility	378:390	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	6	14	theme	overall	717:723	arg1	structure					725:733	the overall structure	713:733	the overall structure of ORP	713:740	Besides, the overall structure of ORP was not damaged after digestion.					
33142599	1	15	theme	Oudemansiella	189:201	arg1	radicata					203:210	Oudemansiella radicata	189:210	Oudemansiella radicata	189:210	Oudemansiella radicata is a commercialized and nutrient-rich mushroom farmed in China.					
33142599	1	15	theme	Oudemansiella	189:201	arg1	mushroom					250:257	a commercialized and nutrient-rich mushroom	215:257	a commercialized and nutrient-rich mushroom farmed in China	215:273	Oudemansiella radicata is a commercialized and nutrient-rich mushroom farmed in China.					
33142599	2	16	theme	biological	336:345	arg1	properties					347:356	strong biological properties	329:356	strong biological properties	329:356	Polysaccharides (ORP) found in this mushroom possess strong biological properties.					
33142599	7	17	theme	several	861:867	arg1	acids					887:891	several short-chain fatty acids	861:891	several short-chain fatty acids	861:891	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	3	18	theme	gut	435:437	arg1	composition					450:460	gut microbiota composition	435:460	gut microbiota composition	435:460	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	0	19	theme	In	0:1	arg1	digestion					33:41	In vitro saliva-gastrointestinal digestion	0:41	In vitro saliva-gastrointestinal digestion	0:41	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	7	20	theme	gut	813:815	arg1	microbiota					817:826	gut microbiota	813:826	gut microbiota	813:826	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	2	21	theme	strong	329:334	arg1	properties					347:356	strong biological properties	329:356	strong biological properties	329:356	Polysaccharides (ORP) found in this mushroom possess strong biological properties.					
33142599	7	22	theme	fermentation	839:850	arg1	process					852:858	the fermentation process	835:858	the fermentation process	835:858	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	3	23	theme	microbiota	439:448	arg1	composition					450:460	gut microbiota composition	435:460	gut microbiota composition	435:460	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	5	24	theme	free	632:635	arg1	monosaccharide					637:650	no free monosaccharide	629:650	no free monosaccharide	629:650	However, no free monosaccharide was detected, indicating that ORP was indigestible.					
33142599	0	25	theme	saliva-gastrointestinal	9:31	arg1	digestion					33:41	In vitro saliva-gastrointestinal digestion	0:41	In vitro saliva-gastrointestinal digestion	0:41	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	8	26	theme	Bacteroides	1078:1088	arg1	abundances					1064:1073	the relative abundances	1051:1073	the relative abundances of Bacteroides and Parabacteroides	1051:1108	Notably, ORP could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Parabacteroides.					
33142599	9	27	theme	gut	1227:1229	arg1	health					1231:1236	gut health	1227:1236	gut health	1227:1236	These results suggest that ORP can be used as a functional food to improve health and prevent diseases by promoting gut health.					
33142599	1	28	theme	commercialized	217:230	arg1	radicata					203:210	Oudemansiella radicata	189:210	Oudemansiella radicata	189:210	Oudemansiella radicata is a commercialized and nutrient-rich mushroom farmed in China.					
33142599	1	28	theme	commercialized	217:230	arg1	mushroom					250:257	a commercialized and nutrient-rich mushroom	215:257	a commercialized and nutrient-rich mushroom farmed in China	215:273	Oudemansiella radicata is a commercialized and nutrient-rich mushroom farmed in China.					
33142599	0	29	theme	fecal	47:51	arg1	fermentation					53:64	fecal fermentation	47:64	fecal fermentation of Oudemansiella radicata polysaccharides	47:106	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	4	30	theme	ORP	579:581	arg1	weight					569:574	the molecular weight	555:574	the molecular weight of ORP	555:581	Results showed that the molecular weight of ORP decreased after simulated digestion.					
33142599	7	31	used	utilized	801:808	arg2	ORP					780:782	ORP	780:782	ORP	780:782	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	8	32	theme	Parabacteroides	1094:1108	arg1	abundances					1064:1073	the relative abundances	1051:1073	the relative abundances of Bacteroides and Parabacteroides	1051:1108	Notably, ORP could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Parabacteroides.					
33142599	1	33	theme	nutrient-rich	236:248	arg1	radicata					203:210	Oudemansiella radicata	189:210	Oudemansiella radicata	189:210	Oudemansiella radicata is a commercialized and nutrient-rich mushroom farmed in China.					
33142599	1	33	theme	nutrient-rich	236:248	arg1	mushroom					250:257	a commercialized and nutrient-rich mushroom	215:257	a commercialized and nutrient-rich mushroom farmed in China	215:273	Oudemansiella radicata is a commercialized and nutrient-rich mushroom farmed in China.					
33142599	2	34	contain	possess	321:327	arg1	Polysaccharides					276:290	Polysaccharides	276:290	Polysaccharides (ORP) found in this mushroom	276:319	Polysaccharides (ORP) found in this mushroom possess strong biological properties.					
33142599	2	34	contain	possess	321:327	arg1	ORP					293:295	ORP	293:295	ORP	293:295	Polysaccharides (ORP) found in this mushroom possess strong biological properties.					
33142599	2	34	contain	possess	321:327	arg2	properties					347:356	strong biological properties	329:356	strong biological properties	329:356	Polysaccharides (ORP) found in this mushroom possess strong biological properties.					
33142599	3	35	from	digestibility	378:390	arg1	composition					450:460	gut microbiota composition	435:460	gut microbiota composition	435:460	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	3	36	theme	fermentation	505:516	arg1	system					518:523	fermentation system	505:523	fermentation system	505:523	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	4	37	theme	molecular	559:567	arg1	weight					569:574	the molecular weight	555:574	the molecular weight of ORP	555:581	Results showed that the molecular weight of ORP decreased after simulated digestion.					
33142599	1	38	from	China	269:273	arg1	farmed					259:264	farmed	259:264	farmed	259:264	Oudemansiella radicata is a commercialized and nutrient-rich mushroom farmed in China.					
33142599	2	39	located	found	298:302	arg2	ORP					293:295	ORP	293:295	ORP	293:295	Polysaccharides (ORP) found in this mushroom possess strong biological properties.					
33142599	2	39	located	found	298:302	arg2	Polysaccharides					276:290	Polysaccharides	276:290	Polysaccharides (ORP) found in this mushroom	276:319	Polysaccharides (ORP) found in this mushroom possess strong biological properties.					
33142599	2	39	located	found	298:302	arg1	mushroom					312:319	this mushroom	307:319	this mushroom	307:319	Polysaccharides (ORP) found in this mushroom possess strong biological properties.					
33142599	0	40	theme	microbiota	177:186	arg1	modulation					155:164	the modulation	151:164	the modulation of the gut microbiota	151:186	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	0	41	from	profile	129:135	arg1	modulation					155:164	the modulation	151:164	the modulation of the gut microbiota	151:186	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	9	42	used	used	1149:1152	arg2	food					1170:1173	a functional food	1157:1173	a functional food	1157:1173	These results suggest that ORP can be used as a functional food to improve health and prevent diseases by promoting gut health.					
33142599	9	42	used	used	1149:1152	arg2	ORP					1138:1140	ORP	1138:1140	ORP	1138:1140	These results suggest that ORP can be used as a functional food to improve health and prevent diseases by promoting gut health.					
33142599	7	43	theme	propionic	919:927	arg1	acid					913:916	acetic acid	906:916	acetic acid	906:916	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	43	theme	propionic	919:927	arg1	acid					929:932	propionic acid	919:932	propionic acid	919:932	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	43	theme	propionic	919:927	arg1	acid					948:951	n-butyric acid	938:951	n-butyric acid	938:951	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	43	theme	propionic	919:927	arg1	products					967:974	the main products	958:974	the main products	958:974	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	0	44	theme	gut	173:175	arg1	microbiota					177:186	the gut microbiota	169:186	the gut microbiota	169:186	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	3	45	dep	digestibility	378:390	arg1	the					374:376	the	374:376	the	374:376	In this study, the digestibility and fermentation of ORP and its effects on gut microbiota composition were examined in a simulated digestion and fermentation system in vitro.					
33142599	7	46	theme	acetic	906:911	arg1	acid					913:916	acetic acid	906:916	acetic acid	906:916	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	46	theme	acetic	906:911	arg1	acid					929:932	propionic acid	919:932	propionic acid	919:932	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	46	theme	acetic	906:911	arg1	acid					948:951	n-butyric acid	938:951	n-butyric acid	938:951	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	7	46	theme	acetic	906:911	arg1	products					967:974	the main products	958:974	the main products	958:974	When ORP was degraded and utilized by gut microbiota during the fermentation process, several short-chain fatty acids were formed, acetic acid, propionic acid and n-butyric acid were the main products.					
33142599	1	47	theme	farmed	259:264	arg1	radicata					203:210	Oudemansiella radicata	189:210	Oudemansiella radicata	189:210	Oudemansiella radicata is a commercialized and nutrient-rich mushroom farmed in China.					
33142599	1	47	theme	farmed	259:264	arg1	mushroom					250:257	a commercialized and nutrient-rich mushroom	215:257	a commercialized and nutrient-rich mushroom farmed in China	215:273	Oudemansiella radicata is a commercialized and nutrient-rich mushroom farmed in China.					
33142599	0	48	theme	radicata	83:90	arg1	polysaccharides					92:106	Oudemansiella radicata polysaccharides	69:106	Oudemansiella radicata polysaccharides	69:106	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	0	49	theme	Oudemansiella	69:81	arg1	polysaccharides					92:106	Oudemansiella radicata polysaccharides	69:106	Oudemansiella radicata polysaccharides	69:106	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	0	50	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
33142599	9	51	theme	functional	1159:1168	arg1	ORP					1138:1140	ORP	1138:1140	ORP	1138:1140	These results suggest that ORP can be used as a functional food to improve health and prevent diseases by promoting gut health.					
33142599	9	51	theme	functional	1159:1168	arg1	food					1170:1173	a functional food	1157:1173	a functional food	1157:1173	These results suggest that ORP can be used as a functional food to improve health and prevent diseases by promoting gut health.					
33142599	0	52	from	effect	141:146	arg1	modulation					155:164	the modulation	151:164	the modulation of the gut microbiota	151:186	In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota.					
34793464	7	0	theme	polysaccharide	1514:1527	arg1	compositions					1529:1540	the polysaccharide compositions	1510:1540	the polysaccharide compositions	1510:1540	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	8	1	theme	abundant	1894:1901	arg1	Units					1931:1935	Operational Taxonomic Units	1909:1935	Operational Taxonomic Units	1909:1935	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	1	theme	abundant	1894:1901	arg1	OTUs					1903:1906	the most abundant OTUs	1885:1906	the most abundant OTUs (Operational Taxonomic Units)	1885:1936	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	2	theme	overlapping	1747:1757	arg1	OTUs					1759:1762	The overlapping OTUs	1743:1762	The overlapping OTUs across all the RPMs	1743:1782	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	3	dep	production	2022:2031	arg1	development					2059:2069	development	2059:2069	development	2059:2069	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	2	4	from	farm	319:322	arg1	RPM					283:285	RPM	283:285	RPM	283:285	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	2	4	from	farm	319:322	arg1	China					337:341	China	337:341	China	337:341	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	2	4	from	farm	319:322	arg1	resource					405:412	a promising natural bioflocculant resource	371:412	a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria	371:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	4	5	theme	comparable	1063:1072	arg1	FRs					1099:1101	FRs	1099:1101	FRs	1099:1101	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	4	5	theme	comparable	1063:1072	arg1	activities					1087:1096	comparable flocculation activities	1063:1096	comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1	1063:1159	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	3	6	theme	flocculation	543:554	arg1	activity					556:563	RPM flocculation activity	539:563	RPM flocculation activity	539:563	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	8	7	contain	contained	1854:1862	arg1	OTUs					1759:1762	The overlapping OTUs	1743:1762	The overlapping OTUs across all the RPMs	1743:1782	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	7	contain	contained	1854:1862	arg2	majority					1873:1880	the vast majority	1864:1880	the vast majority of the most abundant OTUs (Operational Taxonomic Units)	1864:1936	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	7	8	theme	similarity	1485:1494	arg1	clustering					1496:1505	The similarity clustering	1481:1505	The similarity clustering of the polysaccharide compositions	1481:1540	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	8	9	theme	Taxonomic	1921:1929	arg1	Units					1931:1935	Operational Taxonomic Units	1909:1935	Operational Taxonomic Units	1909:1935	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	9	theme	Taxonomic	1921:1929	arg1	OTUs					1903:1906	the most abundant OTUs	1885:1906	the most abundant OTUs (Operational Taxonomic Units)	1885:1936	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	6	10	theme	bacterial	1449:1457	arg1	structures					1469:1478	bacterial community structures	1449:1478	bacterial community structures	1449:1478	The geographical distribution led to certain variation in bacterial community structures.					
34793464	3	11	from	locations	824:832	arg1	survey					784:789	an extensive survey	771:789	an extensive survey of RPMs from four representative locations along the coast of China	771:857	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	3	11	from	locations	824:832	arg1	RPMs					794:797	RPMs	794:797	RPMs from four representative locations along the coast of China	794:857	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	8	12	from	sample	1843:1848	arg1	%					1808:1808	44.6-62.22%	1798:1808	44.6-62.22% of the overall sequences in each sample	1798:1848	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	12	from	sample	1843:1848	arg1	sequences					1825:1833	the overall sequences	1813:1833	the overall sequences in each sample	1813:1848	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	1	13	theme	typical	202:208	arg1	mud					187:189	Ruditapes philippinarum conglutination mud	148:189	Ruditapes philippinarum conglutination mud (RPM)	148:195	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	1	13	theme	typical	202:208	arg1	by-product					216:225	a typical waste by-product	200:225	a typical waste by-product from manila clam R. philippinarum aquaculture	200:271	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	0	14	theme	China	141:145	arg1	coast					132:136	the coast	128:136	the coast of China	128:145	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	8	15	theme	core	1957:1960	arg1	responsible					1991:2001	responsible	1991:2001	responsible	1991:2001	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	15	theme	core	1957:1960	arg1	"					1972:1972	a common "core microbiome"	1947:1972	a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development	1947:2069	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	3	16	theme	manila	586:591	arg1	clam					593:596	the manila clam	582:596	the manila clam across a wide geographical range or only the Zhoushan location	582:659	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	8	17	theme	overall	1817:1823	arg1	sequences					1825:1833	the overall sequences	1813:1833	the overall sequences in each sample	1813:1848	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	4	18	theme	fresh	1168:1172	arg1	RPMs					1174:1177	fresh RPMs	1168:1177	fresh RPMs from Zhoushan	1168:1191	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	7	19	theme	respective	1643:1652	arg1	foundation					1689:1698	the foundation	1685:1698	the foundation of the flocculation activity for all RPMs	1685:1740	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	7	19	theme	respective	1643:1652	arg1	communities					1664:1674	respective bacterial communities	1643:1674	respective bacterial communities	1643:1674	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	7	19	theme	respective	1643:1652	arg1	polysaccharides					1623:1637	polysaccharides	1623:1637	polysaccharides	1623:1637	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	4	20	theme	Frozen	980:985	arg1	samples					1001:1007	Frozen preserved RPM samples	980:1007	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang	980:1051	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	3	21	theme	wide	607:610	arg1	range					625:629	a wide geographical range	605:629	a wide geographical range	605:629	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	1	22	theme	philippinarum	158:170	arg1	by-product					216:225	a typical waste by-product	200:225	a typical waste by-product from manila clam R. philippinarum aquaculture	200:271	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	1	22	theme	philippinarum	158:170	arg1	RPM					192:194	RPM	192:194	RPM	192:194	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	1	22	theme	philippinarum	158:170	arg1	mud					187:189	Ruditapes philippinarum conglutination mud	148:189	Ruditapes philippinarum conglutination mud (RPM)	148:195	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	4	23	theme	RPM	997:999	arg1	samples					1001:1007	Frozen preserved RPM samples	980:1007	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang	980:1051	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	0	24	theme	mud	89:91	arg1	basis					61:65	basis	61:65	basis of the conglutination mud from Ruditapes philippinarum along the coast of China	61:145	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	4	25	from	Zhanjiang	1043:1051	arg1	samples					1001:1007	Frozen preserved RPM samples	980:1007	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang	980:1051	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	3	26	theme	RPMs	794:797	arg1	survey					784:789	an extensive survey	771:789	an extensive survey of RPMs from four representative locations along the coast of China	771:857	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	8	27	theme	polysaccharide	2007:2020	arg1	production					2022:2031	polysaccharide production	2007:2031	polysaccharide production	2007:2031	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	28	theme	vast	1868:1871	arg1	majority					1873:1880	the vast majority	1864:1880	the vast majority of the most abundant OTUs (Operational Taxonomic Units)	1864:1936	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	0	29	from	basis	61:65	arg1	philippinarum					108:120	Ruditapes philippinarum	98:120	Ruditapes philippinarum	98:120	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	4	30	theme	flocculation	1217:1228	arg1	activity					1230:1237	a much higher flocculation activity	1203:1237	a much higher flocculation activity of 91.34±1.18%	1203:1252	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	2	31	theme	flocculating	438:449	arg1	polysaccharides					451:465	effective flocculating polysaccharides	428:465	effective flocculating polysaccharides from the clam associated bacteria	428:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	0	32	theme	conglutination	74:87	arg1	mud					89:91	the conglutination mud	70:91	the conglutination mud from Ruditapes philippinarum along the coast of China	70:145	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	5	33	theme	similar	1338:1344	arg1	constitutions					1361:1373	similar monosaccharide constitutions	1338:1373	similar monosaccharide constitutions	1338:1373	Polysaccharide extracts from the four locations showed similar monosaccharide constitutions to some extent.					
34793464	0	34	theme	Ubiquitous	0:9	arg1	activity					24:31	Ubiquitous flocculation activity	0:31	Ubiquitous flocculation activity	0:31	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	3	35	dep	intent	510:515	arg1	explore					669:675	explore	669:675	explore the flocculation production basis	669:709	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	3	35	dep	intent	510:515	arg1	figure					520:525	figure	520:525	figure	520:525	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	3	36	theme	polysaccharide	915:928	arg1	constitution					930:941	polysaccharide constitution	915:941	polysaccharide constitution	915:941	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	7	37	from	RPMs	1601:1604	arg1	structures					1585:1594	bacterial community structures	1565:1594	bacterial community structures from RPMs	1565:1604	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	7	38	theme	community	1575:1583	arg1	structures					1585:1594	bacterial community structures	1565:1594	bacterial community structures from RPMs	1565:1604	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	7	39	theme	flocculation	1707:1718	arg1	activity					1720:1727	the flocculation activity	1703:1727	the flocculation activity	1703:1727	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	2	40	theme	clam	476:479	arg1	bacteria					492:499	the clam associated bacteria	472:499	the clam associated bacteria	472:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	3	41	theme	Zhoushan	643:650	arg1	location					652:659	only the Zhoushan location	634:659	only the Zhoushan location	634:659	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	2	42	theme	natural	383:389	arg1	RPM					283:285	RPM	283:285	RPM	283:285	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	2	42	theme	natural	383:389	arg1	resource					405:412	a promising natural bioflocculant resource	371:412	a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria	371:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	0	43	theme	flocculation	37:48	arg1	production					50:59	flocculation production	37:59	flocculation production	37:59	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	0	44	dep	basis	61:65	arg1	activity					24:31	Ubiquitous flocculation activity	0:31	Ubiquitous flocculation activity	0:31	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	0	44	dep	basis	61:65	arg1	production					50:59	flocculation production	37:59	flocculation production	37:59	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	3	45	theme	community	957:965	arg1	composition					967:977	bacterial community composition	947:977	bacterial community composition	947:977	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	3	46	from	survey	784:789	arg1	locations					824:832	four representative locations	804:832	four representative locations along the coast of China	804:857	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	4	47	dep	exhibited	1193:1201	arg1	while					1162:1166	while	1162:1166	while	1162:1166	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	7	48	theme	compositions	1529:1540	arg1	clustering					1496:1505	The similarity clustering	1481:1505	The similarity clustering of the polysaccharide compositions	1481:1540	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	4	49	theme	%	1252:1252	arg1	activity					1230:1237	a much higher flocculation activity	1203:1237	a much higher flocculation activity of 91.34±1.18%	1203:1252	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	1	50	theme	clam	239:242	arg1	aquaculture					261:271	manila clam R. philippinarum aquaculture	232:271	manila clam R. philippinarum aquaculture	232:271	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	3	51	theme	production	694:703	arg1	basis					705:709	the flocculation production basis	677:709	the flocculation production basis	677:709	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	8	52	theme	OTUs	1903:1906	arg1	majority					1873:1880	the vast majority	1864:1880	the vast majority of the most abundant OTUs (Operational Taxonomic Units)	1864:1936	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	1	53	theme	philippinarum	247:259	arg1	aquaculture					261:271	manila clam R. philippinarum aquaculture	232:271	manila clam R. philippinarum aquaculture	232:271	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	4	54	theme	flocculation	1074:1085	arg1	FRs					1099:1101	FRs	1099:1101	FRs	1099:1101	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	4	54	theme	flocculation	1074:1085	arg1	activities					1087:1096	comparable flocculation activities	1063:1096	comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1	1063:1159	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	8	55	theme	Operational	1909:1919	arg1	Units					1931:1935	Operational Taxonomic Units	1909:1935	Operational Taxonomic Units	1909:1935	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	55	theme	Operational	1909:1919	arg1	OTUs					1903:1906	the most abundant OTUs	1885:1906	the most abundant OTUs (Operational Taxonomic Units)	1885:1936	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	2	56	contain	contains	419:426	arg1	RPM					283:285	RPM	283:285	RPM	283:285	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	2	56	contain	contains	419:426	arg2	polysaccharides					451:465	effective flocculating polysaccharides	428:465	effective flocculating polysaccharides from the clam associated bacteria	428:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	2	56	contain	contains	419:426	arg1	resource					405:412	a promising natural bioflocculant resource	371:412	a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria	371:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	3	57	theme	RPM	539:541	arg1	activity					556:563	RPM flocculation activity	539:563	RPM flocculation activity	539:563	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	4	58	theme	preserved	987:995	arg1	samples					1001:1007	Frozen preserved RPM samples	980:1007	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang	980:1051	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	2	59	theme	aquaculture	307:317	arg1	farm					319:322	an aquaculture farm	304:322	an aquaculture farm in Zhoushan, China	304:341	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	8	60	from	%	1808:1808	arg1	sample					1843:1848	each sample	1838:1848	each sample	1838:1848	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	2	61	from	bacteria	492:499	arg1	polysaccharides					451:465	effective flocculating polysaccharides	428:465	effective flocculating polysaccharides from the clam associated bacteria	428:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	3	62	theme	exploitation	736:747	arg1	scope					749:753	its exploitation scope	732:753	its exploitation scope	732:753	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	6	63	from	variation	1436:1444	arg1	structures					1469:1478	bacterial community structures	1449:1478	bacterial community structures	1449:1478	The geographical distribution led to certain variation in bacterial community structures.					
34793464	1	64	theme	waste	210:214	arg1	mud					187:189	Ruditapes philippinarum conglutination mud	148:189	Ruditapes philippinarum conglutination mud (RPM)	148:195	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	1	64	theme	waste	210:214	arg1	by-product					216:225	a typical waste by-product	200:225	a typical waste by-product from manila clam R. philippinarum aquaculture	200:271	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	8	65	from	sequences	1825:1833	arg1	sample					1843:1848	each sample	1838:1848	each sample	1838:1848	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	66	theme	microbiome	1962:1971	arg1	responsible					1991:2001	responsible	1991:2001	responsible	1991:2001	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	66	theme	microbiome	1962:1971	arg1	"					1972:1972	a common "core microbiome"	1947:1972	a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development	1947:2069	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	7	67	theme	bacterial	1654:1662	arg1	foundation					1689:1698	the foundation	1685:1698	the foundation of the flocculation activity for all RPMs	1685:1740	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	7	67	theme	bacterial	1654:1662	arg1	communities					1664:1674	respective bacterial communities	1643:1674	respective bacterial communities	1643:1674	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	7	67	theme	bacterial	1654:1662	arg1	polysaccharides					1623:1637	polysaccharides	1623:1637	polysaccharides	1623:1637	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	3	68	theme	geographical	612:623	arg1	range					625:629	a wide geographical range	605:629	a wide geographical range	605:629	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	0	69	from	philippinarum	108:120	arg1	mud					89:91	the conglutination mud	70:91	the conglutination mud from Ruditapes philippinarum along the coast of China	70:145	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	0	69	from	philippinarum	108:120	arg1	basis					61:65	basis	61:65	basis of the conglutination mud from Ruditapes philippinarum along the coast of China	61:145	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	6	70	theme	geographical	1395:1406	arg1	distribution					1408:1419	The geographical distribution	1391:1419	The geographical distribution	1391:1419	The geographical distribution led to certain variation in bacterial community structures.					
34793464	8	71	theme	sequences	1825:1833	arg1	%					1808:1808	44.6-62.22%	1798:1808	44.6-62.22% of the overall sequences in each sample	1798:1848	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	71	theme	sequences	1825:1833	arg1	sequences					1825:1833	the overall sequences	1813:1833	the overall sequences in each sample	1813:1848	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	5	72	from	locations	1321:1329	arg1	extracts					1298:1305	Polysaccharide extracts	1283:1305	Polysaccharide extracts from the four locations	1283:1329	Polysaccharide extracts from the four locations showed similar monosaccharide constitutions to some extent.					
34793464	4	73	theme	g·L-1	1155:1159	arg1	dosage					1143:1148	dosage	1143:1148	dosage of 8 g·L-1	1143:1159	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	3	74	theme	extensive	774:782	arg1	survey					784:789	an extensive survey	771:789	an extensive survey of RPMs from four representative locations along the coast of China	771:857	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	1	75	theme	conglutination	172:185	arg1	by-product					216:225	a typical waste by-product	200:225	a typical waste by-product from manila clam R. philippinarum aquaculture	200:271	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	1	75	theme	conglutination	172:185	arg1	RPM					192:194	RPM	192:194	RPM	192:194	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	1	75	theme	conglutination	172:185	arg1	mud					187:189	Ruditapes philippinarum conglutination mud	148:189	Ruditapes philippinarum conglutination mud (RPM)	148:195	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	2	76	from	clam	296:299	arg1	RPM					283:285	RPM	283:285	RPM	283:285	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	2	76	from	clam	296:299	arg1	resource					405:412	a promising natural bioflocculant resource	371:412	a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria	371:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	2	77	theme	associated	481:490	arg1	bacteria					492:499	the clam associated bacteria	472:499	the clam associated bacteria	472:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	4	78	theme	higher	1210:1215	arg1	activity					1230:1237	a much higher flocculation activity	1203:1237	a much higher flocculation activity of 91.34±1.18%	1203:1252	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	3	79	theme	representative	809:822	arg1	locations					824:832	four representative locations	804:832	four representative locations along the coast of China	804:857	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	3	80	theme	China	853:857	arg1	coast					844:848	the coast	840:848	the coast of China	840:857	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	8	81	theme	common	1949:1954	arg1	responsible					1991:2001	responsible	1991:2001	responsible	1991:2001	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	81	theme	common	1949:1954	arg1	"					1972:1972	a common "core microbiome"	1947:1972	a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development	1947:2069	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	8	82	theme	flocculation	2037:2048	arg1	activity					2050:2057	flocculation activity	2037:2057	flocculation activity	2037:2057	The overlapping OTUs across all the RPMs accounted for 44.6-62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.					
34793464	3	83	theme	flocculation	892:903	arg1	activity					905:912	their flocculation activity	886:912	their flocculation activity	886:912	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	0	84	theme	flocculation	11:22	arg1	activity					24:31	Ubiquitous flocculation activity	0:31	Ubiquitous flocculation activity	0:31	Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China.					
34793464	2	85	theme	effective	428:436	arg1	polysaccharides					451:465	effective flocculating polysaccharides	428:465	effective flocculating polysaccharides from the clam associated bacteria	428:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	5	86	theme	Polysaccharide	1283:1296	arg1	extracts					1298:1305	Polysaccharide extracts	1283:1305	Polysaccharide extracts from the four locations	1283:1329	Polysaccharide extracts from the four locations showed similar monosaccharide constitutions to some extent.					
34793464	4	87	from	Weihai	1032:1037	arg1	samples					1001:1007	Frozen preserved RPM samples	980:1007	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang	980:1051	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	4	88	from	Zhoushan	1184:1191	arg1	RPMs					1174:1177	fresh RPMs	1168:1177	fresh RPMs from Zhoushan	1168:1191	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	4	89	theme	frozen	1263:1268	arg1	counterpart					1270:1280	its frozen counterpart	1259:1280	its frozen counterpart	1259:1280	Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart.					
34793464	2	90	theme	bioflocculant	391:403	arg1	RPM					283:285	RPM	283:285	RPM	283:285	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	2	90	theme	bioflocculant	391:403	arg1	resource					405:412	a promising natural bioflocculant resource	371:412	a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria	371:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	6	91	theme	certain	1428:1434	arg1	variation					1436:1444	certain variation	1428:1444	certain variation in bacterial community structures	1428:1478	The geographical distribution led to certain variation in bacterial community structures.					
34793464	7	92	theme	activity	1720:1727	arg1	foundation					1689:1698	the foundation	1685:1698	the foundation of the flocculation activity for all RPMs	1685:1740	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	7	92	theme	activity	1720:1727	arg1	communities					1664:1674	respective bacterial communities	1643:1674	respective bacterial communities	1643:1674	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	7	92	theme	activity	1720:1727	arg1	polysaccharides					1623:1637	polysaccharides	1623:1637	polysaccharides	1623:1637	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	2	93	theme	promising	373:381	arg1	RPM					283:285	RPM	283:285	RPM	283:285	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	2	93	theme	promising	373:381	arg1	resource					405:412	a promising natural bioflocculant resource	371:412	a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria	371:499	However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria.					
34793464	7	94	theme	bacterial	1565:1573	arg1	structures					1585:1594	bacterial community structures	1565:1594	bacterial community structures from RPMs	1565:1604	The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs.					
34793464	1	95	from	aquaculture	261:271	arg1	mud					187:189	Ruditapes philippinarum conglutination mud	148:189	Ruditapes philippinarum conglutination mud (RPM)	148:195	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	1	95	from	aquaculture	261:271	arg1	by-product					216:225	a typical waste by-product	200:225	a typical waste by-product from manila clam R. philippinarum aquaculture	200:271	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	3	96	theme	bacterial	947:955	arg1	composition					967:977	bacterial community composition	947:977	bacterial community composition	947:977	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	5	97	theme	monosaccharide	1346:1359	arg1	constitutions					1361:1373	similar monosaccharide constitutions	1338:1373	similar monosaccharide constitutions	1338:1373	Polysaccharide extracts from the four locations showed similar monosaccharide constitutions to some extent.					
34793464	1	98	theme	manila	232:237	arg1	aquaculture					261:271	manila clam R. philippinarum aquaculture	232:271	manila clam R. philippinarum aquaculture	232:271	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
34793464	3	99	theme	flocculation	681:692	arg1	basis					705:709	the flocculation production basis	677:709	the flocculation production basis	677:709	With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition.					
34793464	6	100	theme	community	1459:1467	arg1	structures					1469:1478	bacterial community structures	1449:1478	bacterial community structures	1449:1478	The geographical distribution led to certain variation in bacterial community structures.					
34793464	1	101	theme	R.	244:245	arg1	aquaculture					261:271	manila clam R. philippinarum aquaculture	232:271	manila clam R. philippinarum aquaculture	232:271	Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture.					
31900724	0	0	theme	ion	103:105	arg1	spectrometry					117:128	liquid chromatography-electrospray ion trap mass spectrometry	68:128	liquid chromatography-electrospray ion trap mass spectrometry	68:128	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	6	1	theme	CS/DS	917:921	arg1	chains					923:928	CS/DS chains	917:928	CS/DS chains	917:928	The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths.					
31900724	4	2	theme	high	651:654	arg1	LC-MS-ITTOF					733:743	LC-MS-ITTOF	733:743	LC-MS-ITTOF	733:743	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	2	theme	high	651:654	arg1	spectrometry					719:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	651:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	651:744	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	0	3	theme	chromatography-electrospray	75:101	arg1	spectrometry					117:128	liquid chromatography-electrospray ion trap mass spectrometry	68:128	liquid chromatography-electrospray ion trap mass spectrometry	68:128	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	0	4	from	analysis	11:18	arg1	asini					59:63	Colla corii asini	47:63	Colla corii asini	47:63	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	5	5	located	detected	798:805	arg1	fractions					831:839	the three lower salt fractions	810:839	the three lower salt fractions from anion-exchange chromatography	810:874	The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography.					
31900724	5	5	located	detected	798:805	arg2	disaccharides					779:791	CS/DS/HA disaccharides	770:791	CS/DS/HA disaccharides	770:791	The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography.					
31900724	3	6	theme	possible	464:471	arg1	characteristics					484:498	their possible structural characteristics	458:498	their possible structural characteristics in CCA	458:505	However, their possible structural characteristics in CCA are not clear.					
31900724	0	7	theme	mass	112:115	arg1	spectrometry					117:128	liquid chromatography-electrospray ion trap mass spectrometry	68:128	liquid chromatography-electrospray ion trap mass spectrometry	68:128	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	7	8	from	amount	1064:1069	arg1	CCA					1082:1084	CCA	1082:1084	CCA	1082:1084	The quantitative analysis first revealed that the amount of GAGs in CCA varied significantly in total and in each fraction.					
31900724	0	9	theme	trap	107:110	arg1	spectrometry					117:128	liquid chromatography-electrospray ion trap mass spectrometry	68:128	liquid chromatography-electrospray ion trap mass spectrometry	68:128	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	0	10	from	asini	59:63	arg1	glycosaminoglycans					23:40	glycosaminoglycans	23:40	glycosaminoglycans from Colla corii asini	23:63	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	0	10	from	asini	59:63	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.	0:129	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	2	11	theme	dermatan	272:279	arg1	DS					290:291	DS	290:291	DS	290:291	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	11	theme	dermatan	272:279	arg1	classes					344:350	structurally complex classes	323:350	structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities	323:446	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	11	theme	dermatan	272:279	arg1	sulfate					281:287	dermatan sulfate	272:287	dermatan sulfate (DS)	272:292	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	11	theme	dermatan	272:279	arg1	acid					309:312	hyaluronic acid	298:312	hyaluronic acid (HA)	298:317	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	11	theme	dermatan	272:279	arg1	sulfate					258:264	Chondroitin sulfate	246:264	Chondroitin sulfate (CS)	246:269	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	4	12	theme	chromatography-ion	675:692	arg1	LC-MS-ITTOF					733:743	LC-MS-ITTOF	733:743	LC-MS-ITTOF	733:743	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	12	theme	chromatography-ion	675:692	arg1	spectrometry					719:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	651:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	651:744	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	13	theme	liquid	668:673	arg1	LC-MS-ITTOF					733:743	LC-MS-ITTOF	733:743	LC-MS-ITTOF	733:743	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	13	theme	liquid	668:673	arg1	spectrometry					719:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	651:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	651:744	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	5	14	theme	lower	820:824	arg1	fractions					831:839	the three lower salt fractions	810:839	the three lower salt fractions from anion-exchange chromatography	810:874	The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography.					
31900724	3	15	from	characteristics	484:498	arg1	CCA					503:505	CCA	503:505	CCA	503:505	However, their possible structural characteristics in CCA are not clear.					
31900724	2	16	theme	wide	412:415	arg1	range					417:421	a wide range	410:421	a wide range of biological activities	410:446	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	6	17	theme	sulfation	881:889	arg1	patterns					891:898	The sulfation patterns	877:898	The sulfation patterns	877:898	The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths.					
31900724	2	18	theme	biological	426:435	arg1	activities					437:446	biological activities	426:446	biological activities	426:446	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	19	theme	glycosaminoglycans	355:372	arg1	sulfate					258:264	Chondroitin sulfate	246:264	Chondroitin sulfate (CS)	246:269	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	19	theme	glycosaminoglycans	355:372	arg1	classes					344:350	structurally complex classes	323:350	structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities	323:446	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	19	theme	glycosaminoglycans	355:372	arg1	sulfate					281:287	dermatan sulfate	272:287	dermatan sulfate (DS)	272:292	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	19	theme	glycosaminoglycans	355:372	arg1	acid					309:312	hyaluronic acid	298:312	hyaluronic acid (HA)	298:317	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	20	theme	activities	437:446	arg1	range					417:421	a wide range	410:421	a wide range of biological activities	410:446	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	7	21	theme	GAGs	1074:1077	arg1	GAGs					1074:1077	GAGs	1074:1077	GAGs	1074:1077	The quantitative analysis first revealed that the amount of GAGs in CCA varied significantly in total and in each fraction.					
31900724	7	21	theme	GAGs	1074:1077	arg1	amount					1064:1069	the amount	1060:1069	the amount of GAGs in CCA	1060:1084	The quantitative analysis first revealed that the amount of GAGs in CCA varied significantly in total and in each fraction.					
31900724	8	22	theme	biological	1248:1257	arg1	activities					1259:1268	the biological activities	1244:1268	the biological activities of CCA	1244:1275	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of CCA.					
31900724	0	23	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.	0:129	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	6	24	theme	HA	973:974	arg1	chains					976:981	HA chains	973:981	HA chains	973:981	The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths.					
31900724	4	25	theme	mass	714:717	arg1	LC-MS-ITTOF					733:743	LC-MS-ITTOF	733:743	LC-MS-ITTOF	733:743	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	25	theme	mass	714:717	arg1	spectrometry					719:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	651:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	651:744	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	26	theme	disaccharide	608:619	arg1	compositions					621:632	their disaccharide compositions	602:632	their disaccharide compositions	602:632	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	0	27	theme	glycosaminoglycans	23:40	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.	0:129	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	8	28	theme	structural	1149:1158	arg1	information					1160:1170	This novel structural information	1138:1170	This novel structural information	1138:1170	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of CCA.					
31900724	3	29	theme	structural	473:482	arg1	characteristics					484:498	their possible structural characteristics	458:498	their possible structural characteristics in CCA	458:505	However, their possible structural characteristics in CCA are not clear.					
31900724	0	30	theme	Colla	47:51	arg1	asini					59:63	Colla corii asini	47:63	Colla corii asini	47:63	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	4	31	theme	sensitivity	656:666	arg1	LC-MS-ITTOF					733:743	LC-MS-ITTOF	733:743	LC-MS-ITTOF	733:743	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	31	theme	sensitivity	656:666	arg1	spectrometry					719:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	651:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	651:744	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	5	32	theme	salt	826:829	arg1	fractions					831:839	the three lower salt fractions	810:839	the three lower salt fractions from anion-exchange chromatography	810:874	The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography.					
31900724	8	33	theme	polysaccharides	1225:1239	arg1	involvement					1204:1214	the possible involvement	1191:1214	the possible involvement of these polysaccharides in the biological activities of CCA	1191:1275	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of CCA.					
31900724	8	34	theme	possible	1195:1202	arg1	involvement					1204:1214	the possible involvement	1191:1214	the possible involvement of these polysaccharides in the biological activities of CCA	1191:1275	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of CCA.					
31900724	1	35	theme	traditional	203:213	arg1	medicine					236:243	a traditional animal-based Chinese medicine	201:243	a traditional animal-based Chinese medicine	201:243	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	1	35	theme	traditional	203:213	arg1	asini					143:147	Colla corii asini	131:147	Colla corii asini (CCA) made from donkey-hide	131:175	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	5	36	theme	CS/DS/HA	770:777	arg1	disaccharides					779:791	CS/DS/HA disaccharides	770:791	CS/DS/HA disaccharides	770:791	The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography.					
31900724	8	37	theme	novel	1143:1147	arg1	information					1160:1170	This novel structural information	1138:1170	This novel structural information	1138:1170	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of CCA.					
31900724	6	38	from	densities	904:912	arg1	fractions					939:947	these fractions	933:947	these fractions	933:947	The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths.					
31900724	8	39	from	involvement	1204:1214	arg1	activities					1259:1268	the biological activities	1244:1268	the biological activities of CCA	1244:1275	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of CCA.					
31900724	1	40	theme	Colla	131:135	arg1	asini					143:147	Colla corii asini	131:147	Colla corii asini (CCA) made from donkey-hide	131:175	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	1	40	theme	Colla	131:135	arg1	medicine					236:243	a traditional animal-based Chinese medicine	201:243	a traditional animal-based Chinese medicine	201:243	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	1	40	theme	Colla	131:135	arg1	CCA					150:152	CCA	150:152	CCA	150:152	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	0	41	theme	corii	53:57	arg1	asini					59:63	Colla corii asini	47:63	Colla corii asini	47:63	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	1	42	used	used	193:196	arg2	medicine					236:243	a traditional animal-based Chinese medicine	201:243	a traditional animal-based Chinese medicine	201:243	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	1	42	used	used	193:196	arg2	CCA					150:152	CCA	150:152	CCA	150:152	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	1	42	used	used	193:196	arg2	asini					143:147	Colla corii asini	131:147	Colla corii asini (CCA) made from donkey-hide	131:175	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	1	43	theme	corii	137:141	arg1	asini					143:147	Colla corii asini	131:147	Colla corii asini (CCA) made from donkey-hide	131:175	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	1	43	theme	corii	137:141	arg1	medicine					236:243	a traditional animal-based Chinese medicine	201:243	a traditional animal-based Chinese medicine	201:243	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	1	43	theme	corii	137:141	arg1	CCA					150:152	CCA	150:152	CCA	150:152	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	7	44	theme	quantitative	1018:1029	arg1	analysis					1031:1038	The quantitative analysis	1014:1038	The quantitative analysis	1014:1038	The quantitative analysis first revealed that the amount of GAGs in CCA varied significantly in total and in each fraction.					
31900724	4	45	attach	isolated	580:587	arg1	CCA					594:596	CCA	594:596	CCA	594:596	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	45	attach	isolated	580:587	arg2	fractions					541:549	GAG fractions	537:549	GAG fractions containing CS/DS and HA	537:573	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	46	theme	trap/time-of-flight	694:712	arg1	LC-MS-ITTOF					733:743	LC-MS-ITTOF	733:743	LC-MS-ITTOF	733:743	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	46	theme	trap/time-of-flight	694:712	arg1	spectrometry					719:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry	651:730	high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF)	651:744	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	1	47	theme	animal-based	215:226	arg1	medicine					236:243	a traditional animal-based Chinese medicine	201:243	a traditional animal-based Chinese medicine	201:243	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	1	47	theme	animal-based	215:226	arg1	asini					143:147	Colla corii asini	131:147	Colla corii asini (CCA) made from donkey-hide	131:175	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	5	48	from	chromatography	861:874	arg1	fractions					831:839	the three lower salt fractions	810:839	the three lower salt fractions from anion-exchange chromatography	810:874	The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography.					
31900724	2	49	theme	hyaluronic	298:307	arg1	sulfate					258:264	Chondroitin sulfate	246:264	Chondroitin sulfate (CS)	246:269	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	49	theme	hyaluronic	298:307	arg1	classes					344:350	structurally complex classes	323:350	structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities	323:446	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	49	theme	hyaluronic	298:307	arg1	HA					315:316	HA	315:316	HA	315:316	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	49	theme	hyaluronic	298:307	arg1	acid					309:312	hyaluronic acid	298:312	hyaluronic acid (HA)	298:317	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	49	theme	hyaluronic	298:307	arg1	sulfate					281:287	dermatan sulfate	272:287	dermatan sulfate (DS)	272:292	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	1	50	theme	Chinese	228:234	arg1	medicine					236:243	a traditional animal-based Chinese medicine	201:243	a traditional animal-based Chinese medicine	201:243	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	1	50	theme	Chinese	228:234	arg1	asini					143:147	Colla corii asini	131:147	Colla corii asini (CCA) made from donkey-hide	131:175	Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine.					
31900724	0	51	theme	liquid	68:73	arg1	spectrometry					117:128	liquid chromatography-electrospray ion trap mass spectrometry	68:128	liquid chromatography-electrospray ion trap mass spectrometry	68:128	Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry.					
31900724	8	52	theme	CCA	1273:1275	arg1	activities					1259:1268	the biological activities	1244:1268	the biological activities of CCA	1244:1275	This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of CCA.					
31900724	4	53	contain	containing	551:560	arg2	HA					572:573	HA	572:573	HA	572:573	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	53	contain	containing	551:560	arg2	CS/DS					562:566	CS/DS	562:566	CS/DS	562:566	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	4	53	contain	containing	551:560	arg1	fractions					541:549	GAG fractions	537:549	GAG fractions containing CS/DS and HA	537:573	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	2	54	theme	complex	336:342	arg1	sulfate					258:264	Chondroitin sulfate	246:264	Chondroitin sulfate (CS)	246:269	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	54	theme	complex	336:342	arg1	classes					344:350	structurally complex classes	323:350	structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities	323:446	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	54	theme	complex	336:342	arg1	sulfate					281:287	dermatan sulfate	272:287	dermatan sulfate (DS)	272:292	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	54	theme	complex	336:342	arg1	acid					309:312	hyaluronic acid	298:312	hyaluronic acid (HA)	298:317	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	5	55	theme	anion-exchange	846:859	arg1	chromatography					861:874	anion-exchange chromatography	846:874	anion-exchange chromatography	846:874	The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography.					
31900724	4	56	theme	GAG	537:539	arg1	fractions					541:549	GAG fractions	537:549	GAG fractions containing CS/DS and HA	537:573	In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF).					
31900724	6	57	from	patterns	891:898	arg1	fractions					939:947	these fractions	933:947	these fractions	933:947	The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths.					
31900724	6	58	theme	chains	923:928	arg1	patterns					891:898	The sulfation patterns	877:898	The sulfation patterns	877:898	The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths.					
31900724	6	58	theme	chains	923:928	arg1	densities					904:912	densities	904:912	densities	904:912	The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths.					
31900724	2	59	theme	Chondroitin	246:256	arg1	sulfate					258:264	Chondroitin sulfate	246:264	Chondroitin sulfate (CS)	246:269	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	59	theme	Chondroitin	246:256	arg1	classes					344:350	structurally complex classes	323:350	structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities	323:446	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	59	theme	Chondroitin	246:256	arg1	sulfate					281:287	dermatan sulfate	272:287	dermatan sulfate (DS)	272:292	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	59	theme	Chondroitin	246:256	arg1	acid					309:312	hyaluronic acid	298:312	hyaluronic acid (HA)	298:317	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	2	59	theme	Chondroitin	246:256	arg1	CS					267:268	CS	267:268	CS	267:268	Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities.					
31900724	6	60	theme	chain	999:1003	arg1	lengths					1005:1011	their chain lengths	993:1011	their chain lengths	993:1011	The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths.					
33320008	0	0	theme	Type	88:91	arg1	Oligosaccharides					95:110	Type I Oligosaccharides	88:110	Type I Oligosaccharides	88:110	Threshold of Thioglycoside Reactivity Difference Is Critical for Efficient Synthesis of Type I Oligosaccharides by Chemoselective Glycosylation.					
33320008	4	1	theme	aglycon	715:721	arg1	transfer					723:730	aglycon transfer	715:730	aglycon transfer	715:730	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
33320008	6	2	theme	LacNAc	1061:1066	arg1	hexasaccharides					1068:1082	LacNAc hexasaccharides	1061:1082	type I LacNAc hexasaccharides	1054:1082	Because it is challenging to measure the RRVs of tetrasaccharides, anomeric proton chemical shifts were utilized to predict the corresponding RRVs, which consequently explained the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides.					
33320008	1	3	theme	LacNAc	165:170	arg1	3GlcNAc					181:187	Galβ1 → 3GlcNAc	173:187	Galβ1 → 3GlcNAc	173:187	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	1	3	theme	LacNAc	165:170	arg1	oligosaccharides					190:205	type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	158:205	type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	158:205	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	6	4	theme	glycosylations	1018:1031	arg1	outcome					1007:1013	the outcome	1003:1013	the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides	1003:1082	Because it is challenging to measure the RRVs of tetrasaccharides, anomeric proton chemical shifts were utilized to predict the corresponding RRVs, which consequently explained the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides.					
33320008	4	5	theme	RRV	556:558	arg1	difference					560:569	the RRV difference	552:569	the RRV difference between the donors and acceptors	552:602	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
33320008	4	5	theme	RRV	556:558	arg1	more					614:617	more	614:617	more	614:617	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
33320008	7	6	theme	glycan	1134:1139	arg1	chains					1141:1146	glycan chains	1134:1146	glycan chains	1134:1146	The result supported the idea that elongation of glycan chains has to proceed from the reducing to the nonreducing end for a better yield.					
33320008	6	7	theme	tetrasaccharides	875:890	arg1	RRVs					867:870	the RRVs	863:870	the RRVs of tetrasaccharides	863:890	Because it is challenging to measure the RRVs of tetrasaccharides, anomeric proton chemical shifts were utilized to predict the corresponding RRVs, which consequently explained the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides.					
33320008	0	8	theme	Oligosaccharides	95:110	arg1	Synthesis					75:83	Efficient Synthesis	65:83	Efficient Synthesis of Type I Oligosaccharides by Chemoselective Glycosylation	65:142	Threshold of Thioglycoside Reactivity Difference Is Critical for Efficient Synthesis of Type I Oligosaccharides by Chemoselective Glycosylation.					
33320008	2	9	theme	I	289:289	arg1	oligosaccharides					298:313	type I LacNAc oligosaccharides	284:313	type I LacNAc oligosaccharides	284:313	We herein report the efficient synthesis of type I LacNAc oligosaccharides by chemoselective glycosylation.					
33320008	0	10	theme	I	93:93	arg1	Oligosaccharides					95:110	Type I Oligosaccharides	88:110	Type I Oligosaccharides	88:110	Threshold of Thioglycoside Reactivity Difference Is Critical for Efficient Synthesis of Type I Oligosaccharides by Chemoselective Glycosylation.					
33320008	6	11	theme	chemical	909:916	arg1	shifts					918:923	anomeric proton chemical shifts	893:923	anomeric proton chemical shifts	893:923	Because it is challenging to measure the RRVs of tetrasaccharides, anomeric proton chemical shifts were utilized to predict the corresponding RRVs, which consequently explained the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides.					
33320008	3	12	with	donors	432:437	arg1	RRVs					384:387	RRVs	384:387	RRVs	384:387	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	3	12	with	donors	432:437	arg1	values					376:381	16 relative reactivity values	353:381	16 relative reactivity values (RRVs)	353:388	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	2	13	theme	type	284:287	arg1	oligosaccharides					298:313	type I LacNAc oligosaccharides	284:313	type I LacNAc oligosaccharides	284:313	We herein report the efficient synthesis of type I LacNAc oligosaccharides by chemoselective glycosylation.					
33320008	0	14	theme	Chemoselective	115:128	arg1	Glycosylation					130:142	Chemoselective Glycosylation	115:142	Chemoselective Glycosylation	115:142	Threshold of Thioglycoside Reactivity Difference Is Critical for Efficient Synthesis of Type I Oligosaccharides by Chemoselective Glycosylation.					
33320008	6	15	theme	proton	902:907	arg1	shifts					918:923	anomeric proton chemical shifts	893:923	anomeric proton chemical shifts	893:923	Because it is challenging to measure the RRVs of tetrasaccharides, anomeric proton chemical shifts were utilized to predict the corresponding RRVs, which consequently explained the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides.					
33320008	4	16	theme	LacNAc	646:651	arg1	tetrasaccharides					653:668	type I LacNAc tetrasaccharides	639:668	type I LacNAc tetrasaccharides in 72-86% yields	639:685	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
33320008	1	17	theme	Galβ1	173:177	arg1	3GlcNAc					181:187	Galβ1 → 3GlcNAc	173:187	Galβ1 → 3GlcNAc	173:187	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	1	17	theme	Galβ1	173:177	arg1	oligosaccharides					190:205	type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	158:205	type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	158:205	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	3	18	theme	reactivity	365:374	arg1	RRVs					384:387	RRVs	384:387	RRVs	384:387	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	3	18	theme	reactivity	365:374	arg1	values					376:381	16 relative reactivity values	353:381	16 relative reactivity values (RRVs)	353:388	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	6	19	theme	anomeric	893:900	arg1	shifts					918:923	anomeric proton chemical shifts	893:923	anomeric proton chemical shifts	893:923	Because it is challenging to measure the RRVs of tetrasaccharides, anomeric proton chemical shifts were utilized to predict the corresponding RRVs, which consequently explained the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides.					
33320008	5	20	theme	glycans	817:823	arg1	synthesis					797:805	the synthesis	793:805	the synthesis of longer glycans	793:823	The threshold of RRV difference was further applied to plan the synthesis of longer glycans.					
33320008	3	21	with	acceptors	443:451	arg1	RRVs					384:387	RRVs	384:387	RRVs	384:387	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	3	21	with	acceptors	443:451	arg1	values					376:381	16 relative reactivity values	353:381	16 relative reactivity values (RRVs)	353:388	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	1	22	theme	→	179:179	arg1	3GlcNAc					181:187	Galβ1 → 3GlcNAc	173:187	Galβ1 → 3GlcNAc	173:187	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	1	22	theme	→	179:179	arg1	oligosaccharides					190:205	type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	158:205	type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	158:205	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	3	23	theme	measured	390:397	arg1	donors					432:437	measured thiotoluenyl-linked disaccharide donors	390:437	measured thiotoluenyl-linked disaccharide donors	390:437	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	2	24	theme	chemoselective	318:331	arg1	glycosylation					333:345	chemoselective glycosylation	318:345	chemoselective glycosylation	318:345	We herein report the efficient synthesis of type I LacNAc oligosaccharides by chemoselective glycosylation.					
33320008	3	25	theme	thiotoluenyl-linked	399:417	arg1	donors					432:437	measured thiotoluenyl-linked disaccharide donors	390:437	measured thiotoluenyl-linked disaccharide donors	390:437	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	3	26	theme	chemoselective	454:467	arg1	glycosylations					469:482	chemoselective glycosylations	454:482	chemoselective glycosylations	454:482	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	0	27	theme	Reactivity	27:36	arg1	Difference					38:47	Thioglycoside Reactivity Difference	13:47	Thioglycoside Reactivity Difference	13:47	Threshold of Thioglycoside Reactivity Difference Is Critical for Efficient Synthesis of Type I Oligosaccharides by Chemoselective Glycosylation.					
33320008	7	28	theme	chains	1141:1146	arg1	elongation					1120:1129	elongation	1120:1129	elongation of glycan chains	1120:1146	The result supported the idea that elongation of glycan chains has to proceed from the reducing to the nonreducing end for a better yield.					
33320008	2	29	theme	oligosaccharides	298:313	arg1	synthesis					271:279	the efficient synthesis	257:279	the efficient synthesis of type I LacNAc oligosaccharides by chemoselective glycosylation	257:345	We herein report the efficient synthesis of type I LacNAc oligosaccharides by chemoselective glycosylation.					
33320008	1	30	theme	oligosaccharides	190:205	arg1	Synthesis					145:153	Synthesis	145:153	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	145:205	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	0	31	theme	Thioglycoside	13:25	arg1	Reactivity					27:36	Thioglycoside Reactivity	13:36	Thioglycoside Reactivity Difference	13:47	Threshold of Thioglycoside Reactivity Difference Is Critical for Efficient Synthesis of Type I Oligosaccharides by Chemoselective Glycosylation.					
33320008	6	32	dep	type	1054:1057	arg1	hexasaccharides					1068:1082	LacNAc hexasaccharides	1061:1082	type I LacNAc hexasaccharides	1054:1082	Because it is challenging to measure the RRVs of tetrasaccharides, anomeric proton chemical shifts were utilized to predict the corresponding RRVs, which consequently explained the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides.					
33320008	3	33	theme	relative	356:363	arg1	RRVs					384:387	RRVs	384:387	RRVs	384:387	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	3	33	theme	relative	356:363	arg1	values					376:381	16 relative reactivity values	353:381	16 relative reactivity values (RRVs)	353:388	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	2	34	theme	LacNAc	291:296	arg1	oligosaccharides					298:313	type I LacNAc oligosaccharides	284:313	type I LacNAc oligosaccharides	284:313	We herein report the efficient synthesis of type I LacNAc oligosaccharides by chemoselective glycosylation.					
33320008	4	35	from	tetrasaccharides	653:668	arg1	yields					680:685	72-86% yields	673:685	72-86% yields	673:685	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
33320008	3	36	theme	optimal	512:518	arg1	conditions					520:529	optimal conditions	512:529	optimal conditions	512:529	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	7	37	theme	nonreducing	1188:1198	arg1	end					1200:1202	the nonreducing end	1184:1202	the nonreducing end for a better yield	1184:1221	The result supported the idea that elongation of glycan chains has to proceed from the reducing to the nonreducing end for a better yield.					
33320008	4	38	theme	72-86	673:677	arg1	%					678:678	%	678:678	%	678:678	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
33320008	0	39	theme	Difference	38:47	arg1	Threshold					0:8	Threshold	0:8	Threshold of Thioglycoside Reactivity Difference	0:47	Threshold of Thioglycoside Reactivity Difference Is Critical for Efficient Synthesis of Type I Oligosaccharides by Chemoselective Glycosylation.					
33320008	5	40	theme	RRV	750:752	arg1	difference					754:763	RRV difference	750:763	RRV difference	750:763	The threshold of RRV difference was further applied to plan the synthesis of longer glycans.					
33320008	6	41	theme	type	1054:1057	arg1	synthesis					1041:1049	the synthesis	1037:1049	the synthesis of type I LacNAc hexasaccharides	1037:1082	Because it is challenging to measure the RRVs of tetrasaccharides, anomeric proton chemical shifts were utilized to predict the corresponding RRVs, which consequently explained the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides.					
33320008	3	42	theme	disaccharide	419:430	arg1	donors					432:437	measured thiotoluenyl-linked disaccharide donors	390:437	measured thiotoluenyl-linked disaccharide donors	390:437	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	6	43	theme	corresponding	954:966	arg1	RRVs					968:971	the corresponding RRVs	950:971	the corresponding RRVs	950:971	Because it is challenging to measure the RRVs of tetrasaccharides, anomeric proton chemical shifts were utilized to predict the corresponding RRVs, which consequently explained the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides.					
33320008	6	44	used	utilized	930:937	arg2	shifts					918:923	anomeric proton chemical shifts	893:923	anomeric proton chemical shifts	893:923	Because it is challenging to measure the RRVs of tetrasaccharides, anomeric proton chemical shifts were utilized to predict the corresponding RRVs, which consequently explained the outcome of glycosylations for the synthesis of type I LacNAc hexasaccharides.					
33320008	5	45	theme	longer	810:815	arg1	glycans					817:823	longer glycans	810:823	longer glycans	810:823	The threshold of RRV difference was further applied to plan the synthesis of longer glycans.					
33320008	7	46	theme	better	1210:1215	arg1	yield					1217:1221	a better yield	1208:1221	a better yield	1208:1221	The result supported the idea that elongation of glycan chains has to proceed from the reducing to the nonreducing end for a better yield.					
33320008	4	47	theme	transfer	723:730	arg1	occurrence					701:710	minimal occurrence	693:710	minimal occurrence of aglycon transfer	693:730	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
33320008	2	48	theme	efficient	261:269	arg1	synthesis					271:279	the efficient synthesis	257:279	the efficient synthesis of type I LacNAc oligosaccharides by chemoselective glycosylation	257:345	We herein report the efficient synthesis of type I LacNAc oligosaccharides by chemoselective glycosylation.					
33320008	1	49	theme	low	228:230	arg1	yields					232:237	low yields	228:237	low yields	228:237	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	4	50	theme	minimal	693:699	arg1	occurrence					701:710	minimal occurrence	693:710	minimal occurrence of aglycon transfer	693:730	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
33320008	5	51	theme	difference	754:763	arg1	threshold					737:745	The threshold	733:745	The threshold of RRV difference	733:763	The threshold of RRV difference was further applied to plan the synthesis of longer glycans.					
33320008	1	52	theme	type	158:161	arg1	3GlcNAc					181:187	Galβ1 → 3GlcNAc	173:187	Galβ1 → 3GlcNAc	173:187	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	1	52	theme	type	158:161	arg1	oligosaccharides					190:205	type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	158:205	type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	158:205	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	4	53	theme	%	678:678	arg1	yields					680:685	72-86% yields	673:685	72-86% yields	673:685	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
33320008	4	54	theme	I	644:644	arg1	tetrasaccharides					653:668	type I LacNAc tetrasaccharides	639:668	type I LacNAc tetrasaccharides in 72-86% yields	639:685	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
33320008	0	55	theme	Efficient	65:73	arg1	Synthesis					75:83	Efficient Synthesis	65:83	Efficient Synthesis of Type I Oligosaccharides by Chemoselective Glycosylation	65:142	Threshold of Thioglycoside Reactivity Difference Is Critical for Efficient Synthesis of Type I Oligosaccharides by Chemoselective Glycosylation.					
33320008	3	56	link	thiotoluenyl-linked	399:417	arg1	donors					432:437	measured thiotoluenyl-linked disaccharide donors	390:437	measured thiotoluenyl-linked disaccharide donors	390:437	With 16 relative reactivity values (RRVs) measured thiotoluenyl-linked disaccharide donors and acceptors, chemoselective glycosylations were investigated to obtain optimal conditions.					
33320008	1	57	theme	I	163:163	arg1	3GlcNAc					181:187	Galβ1 → 3GlcNAc	173:187	Galβ1 → 3GlcNAc	173:187	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	1	57	theme	I	163:163	arg1	oligosaccharides					190:205	type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	158:205	type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides	158:205	Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields.					
33320008	4	58	theme	type	639:642	arg1	tetrasaccharides					653:668	type I LacNAc tetrasaccharides	639:668	type I LacNAc tetrasaccharides in 72-86% yields	639:685	In these reactions, the RRV difference between the donors and acceptors had to be more than 6311 to obtain type I LacNAc tetrasaccharides in 72-86% yields, with minimal occurrence of aglycon transfer.					
34591399	5	0	theme	Caco-2	888:893	arg1	cells					895:899	LPS-induced Caco-2 cells	876:899	LPS-induced Caco-2 cells	876:899	Anti-inflammatory activity was compared using LPS-induced Caco-2 cells and a rat model.					
34591399	4	1	theme	molar	816:820	arg1	ratios					822:827	different molar ratios	806:827	different molar ratios	806:827	wHEP-1 was composed of mannose, glucose, and galactose in a molar ratio of 1.2:16.9:1:1, whereas wHEP-2 and wHEP-3 were composed of glucose and galactose in different molar ratios.					
34591399	2	2	theme	physical	343:350	arg1	properties					352:361	their chemical and physical properties	324:361	their chemical and physical properties	324:361	This study assessed their chemical and physical properties, molecular weight, monosaccharide compositions, and anti-ulcerative colitis ability to protect Caco-2 cells from lipopolysaccharide (LPS)-induced inflammation.					
34591399	7	3	theme	alternative	1076:1086	arg1	therapies					1088:1096	alternative therapies	1076:1096	alternative therapies	1076:1096	Thus, the polysaccharide from H. erinaceus as a natural material shows potential for the development of alternative therapies.					
34591399	3	4	theme	average	551:557	arg1	5010					610:613	5010	610:613	5010	610:613	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	3	4	theme	average	551:557	arg1	weight					569:574	the average molecular weight	547:574	the average molecular weight of wHEP-1, wHEP-2, and wHEP-3	547:604	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	7	5	theme	therapies	1088:1096	arg1	development					1061:1071	the development	1057:1071	the development of alternative therapies	1057:1096	Thus, the polysaccharide from H. erinaceus as a natural material shows potential for the development of alternative therapies.					
34591399	3	6	theme	molecular	559:567	arg1	5010					610:613	5010	610:613	5010	610:613	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	3	6	theme	molecular	559:567	arg1	weight					569:574	the average molecular weight	547:574	the average molecular weight of wHEP-1, wHEP-2, and wHEP-3	547:604	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	6	7	theme	anti-inflammatory	944:960	arg1	activity					962:969	the best anti-inflammatory activity	935:969	the best anti-inflammatory activity	935:969	wHEP-1 exhibited the best anti-inflammatory activity.					
34591399	0	8	from	Activities	57:66	arg1	Mycelium					102:109	the Mycelium	98:109	the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes)	98:189	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	4	9	dep	composed	660:667	arg1	whereas					738:744	whereas	738:744	whereas	738:744	wHEP-1 was composed of mannose, glucose, and galactose in a molar ratio of 1.2:16.9:1:1, whereas wHEP-2 and wHEP-3 were composed of glucose and galactose in different molar ratios.					
34591399	4	10	from	galactose	793:801	arg1	ratios					822:827	different molar ratios	806:827	different molar ratios	806:827	wHEP-1 was composed of mannose, glucose, and galactose in a molar ratio of 1.2:16.9:1:1, whereas wHEP-2 and wHEP-3 were composed of glucose and galactose in different molar ratios.					
34591399	4	11	theme	1.2:16.9:1:1	724:735	arg1	ratio					715:719	a molar ratio	707:719	a molar ratio of 1.2:16.9:1:1	707:735	wHEP-1 was composed of mannose, glucose, and galactose in a molar ratio of 1.2:16.9:1:1, whereas wHEP-2 and wHEP-3 were composed of glucose and galactose in different molar ratios.					
34591399	2	12	theme	monosaccharide	382:395	arg1	compositions					397:408	monosaccharide compositions	382:408	monosaccharide compositions	382:408	This study assessed their chemical and physical properties, molecular weight, monosaccharide compositions, and anti-ulcerative colitis ability to protect Caco-2 cells from lipopolysaccharide (LPS)-induced inflammation.					
34591399	2	13	theme	Caco-2	458:463	arg1	cells					465:469	Caco-2 cells	458:469	Caco-2 cells	458:469	This study assessed their chemical and physical properties, molecular weight, monosaccharide compositions, and anti-ulcerative colitis ability to protect Caco-2 cells from lipopolysaccharide (LPS)-induced inflammation.					
34591399	4	14	theme	different	806:814	arg1	ratios					822:827	different molar ratios	806:827	different molar ratios	806:827	wHEP-1 was composed of mannose, glucose, and galactose in a molar ratio of 1.2:16.9:1:1, whereas wHEP-2 and wHEP-3 were composed of glucose and galactose in different molar ratios.					
34591399	3	15	theme	wHEP-1	579:584	arg1	5010					610:613	5010	610:613	5010	610:613	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	3	15	theme	wHEP-1	579:584	arg1	weight					569:574	the average molecular weight	547:574	the average molecular weight of wHEP-1, wHEP-2, and wHEP-3	547:604	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	6	16	theme	best	939:942	arg1	activity					962:969	the best anti-inflammatory activity	935:969	the best anti-inflammatory activity	935:969	wHEP-1 exhibited the best anti-inflammatory activity.					
34591399	5	17	theme	rat	907:909	arg1	model					911:915	a rat model	905:915	a rat model	905:915	Anti-inflammatory activity was compared using LPS-induced Caco-2 cells and a rat model.					
34591399	1	18	theme	major	198:202	arg1	wHEP-2					229:234	wHEP-2	229:234	wHEP-2	229:234	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	1	18	theme	major	198:202	arg1	wHEP-3					241:246	wHEP-3	241:246	wHEP-3	241:246	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	1	18	theme	major	198:202	arg1	wHEP-1					221:226	wHEP-1	221:226	wHEP-1	221:226	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	1	18	theme	major	198:202	arg1	polysaccharides					204:218	Three major polysaccharides	192:218	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3)	192:247	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	0	19	theme	Culinary-Medicinal	126:143	arg1	Mushroom					145:152	Lion's Mane Culinary-Medicinal Mushroom	114:152	Lion's Mane Culinary-Medicinal Mushroom	114:152	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	0	19	theme	Culinary-Medicinal	126:143	arg1	erinaceus					164:172	Hericium erinaceus	155:172	Hericium erinaceus (Agaricomycetes)	155:189	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	4	20	theme	molar	709:713	arg1	ratio					715:719	a molar ratio	707:719	a molar ratio of 1.2:16.9:1:1	707:735	wHEP-1 was composed of mannose, glucose, and galactose in a molar ratio of 1.2:16.9:1:1, whereas wHEP-2 and wHEP-3 were composed of glucose and galactose in different molar ratios.					
34591399	2	21	theme	colitis	431:437	arg1	ability					439:445	anti-ulcerative colitis ability	415:445	anti-ulcerative colitis ability	415:445	This study assessed their chemical and physical properties, molecular weight, monosaccharide compositions, and anti-ulcerative colitis ability to protect Caco-2 cells from lipopolysaccharide (LPS)-induced inflammation.					
34591399	0	22	theme	Mane	121:124	arg1	Mushroom					145:152	Lion's Mane Culinary-Medicinal Mushroom	114:152	Lion's Mane Culinary-Medicinal Mushroom	114:152	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	0	22	theme	Mane	121:124	arg1	erinaceus					164:172	Hericium erinaceus	155:172	Hericium erinaceus (Agaricomycetes)	155:189	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	3	23	theme	1118	626:629	arg1	Da					631:632	1118 Da	626:632	1118 Da	626:632	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	2	24	theme	anti-ulcerative	415:429	arg1	ability					439:445	anti-ulcerative colitis ability	415:445	anti-ulcerative colitis ability	415:445	This study assessed their chemical and physical properties, molecular weight, monosaccharide compositions, and anti-ulcerative colitis ability to protect Caco-2 cells from lipopolysaccharide (LPS)-induced inflammation.					
34591399	0	25	theme	Inflammatory	44:55	arg1	Activities					57:66	Anti-Intestinal Inflammatory Activities	28:66	Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes)	28:189	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	1	26	theme	Hericium	284:291	arg1	erinaceus					293:301	Hericium erinaceus	284:301	Hericium erinaceus	284:301	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	7	27	theme	natural	1020:1026	arg1	material					1028:1035	a natural material	1018:1035	a natural material	1018:1035	Thus, the polysaccharide from H. erinaceus as a natural material shows potential for the development of alternative therapies.					
34591399	5	28	theme	Anti-inflammatory	830:846	arg1	activity					848:855	Anti-inflammatory activity	830:855	Anti-inflammatory activity	830:855	Anti-inflammatory activity was compared using LPS-induced Caco-2 cells and a rat model.					
34591399	0	29	theme	Anti-Intestinal	28:42	arg1	Activities					57:66	Anti-Intestinal Inflammatory Activities	28:66	Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes)	28:189	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	1	30	theme	erinaceus	293:301	arg1	mycelium					272:279	the mycelium	268:279	the mycelium of Hericium erinaceus	268:301	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	0	31	theme	Mushroom	145:152	arg1	Mycelium					102:109	the Mycelium	98:109	the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes)	98:189	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	3	32	theme	wHEP-3	599:604	arg1	5010					610:613	5010	610:613	5010	610:613	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	3	32	theme	wHEP-3	599:604	arg1	weight					569:574	the average molecular weight	547:574	the average molecular weight of wHEP-1, wHEP-2, and wHEP-3	547:604	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	4	33	from	glucose	781:787	arg1	ratios					822:827	different molar ratios	806:827	different molar ratios	806:827	wHEP-1 was composed of mannose, glucose, and galactose in a molar ratio of 1.2:16.9:1:1, whereas wHEP-2 and wHEP-3 were composed of glucose and galactose in different molar ratios.					
34591399	3	34	theme	wHEP-2	587:592	arg1	5010					610:613	5010	610:613	5010	610:613	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	3	34	theme	wHEP-2	587:592	arg1	weight					569:574	the average molecular weight	547:574	the average molecular weight of wHEP-1, wHEP-2, and wHEP-3	547:604	The results showed that the average molecular weight of wHEP-1, wHEP-2, and wHEP-3 was 5010, 1812, and 1118 Da, respectively.					
34591399	5	35	theme	LPS-induced	876:886	arg1	cells					895:899	LPS-induced Caco-2 cells	876:899	LPS-induced Caco-2 cells	876:899	Anti-inflammatory activity was compared using LPS-induced Caco-2 cells and a rat model.					
34591399	0	36	theme	Activities	57:66	arg1	Comparison					14:23	Comparison	14:23	Comparison	14:23	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	0	36	theme	Activities	57:66	arg1	Screening					0:8	Screening	0:8	Screening	0:8	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	0	37	theme	Hericium	155:162	arg1	Mushroom					145:152	Lion's Mane Culinary-Medicinal Mushroom	114:152	Lion's Mane Culinary-Medicinal Mushroom	114:152	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	0	37	theme	Hericium	155:162	arg1	erinaceus					164:172	Hericium erinaceus	155:172	Hericium erinaceus (Agaricomycetes)	155:189	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	0	37	theme	Hericium	155:162	arg1	Agaricomycetes					175:188	Agaricomycetes	175:188	Agaricomycetes	175:188	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	2	38	theme	chemical	330:337	arg1	properties					352:361	their chemical and physical properties	324:361	their chemical and physical properties	324:361	This study assessed their chemical and physical properties, molecular weight, monosaccharide compositions, and anti-ulcerative colitis ability to protect Caco-2 cells from lipopolysaccharide (LPS)-induced inflammation.					
34591399	7	39	theme	H.	1002:1003	arg1	erinaceus					1005:1013	H. erinaceus	1002:1013	H. erinaceus	1002:1013	Thus, the polysaccharide from H. erinaceus as a natural material shows potential for the development of alternative therapies.					
34591399	7	40	from	erinaceus	1005:1013	arg1	polysaccharide					982:995	the polysaccharide	978:995	the polysaccharide from H. erinaceus as a natural material	978:1035	Thus, the polysaccharide from H. erinaceus as a natural material shows potential for the development of alternative therapies.					
34591399	0	41	theme	Polysaccharides	77:91	arg1	Activities					57:66	Anti-Intestinal Inflammatory Activities	28:66	Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes)	28:189	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	0	42	from	Mycelium	102:109	arg1	Activities					57:66	Anti-Intestinal Inflammatory Activities	28:66	Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes)	28:189	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	0	42	from	Mycelium	102:109	arg1	Polysaccharides					77:91	Three Polysaccharides	71:91	Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes)	71:189	Screening and Comparison of Anti-Intestinal Inflammatory Activities of Three Polysaccharides from the Mycelium of Lion's Mane Culinary-Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).					
34591399	1	43	dep	polysaccharides	204:218	arg1	wHEP-2					229:234	wHEP-2	229:234	wHEP-2	229:234	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	1	43	dep	polysaccharides	204:218	arg1	wHEP-3					241:246	wHEP-3	241:246	wHEP-3	241:246	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	1	43	dep	polysaccharides	204:218	arg1	wHEP-1					221:226	wHEP-1	221:226	wHEP-1	221:226	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	1	43	dep	polysaccharides	204:218	arg1	polysaccharides					204:218	Three major polysaccharides	192:218	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3)	192:247	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	2	44	theme	molecular	364:372	arg1	weight					374:379	molecular weight	364:379	molecular weight	364:379	This study assessed their chemical and physical properties, molecular weight, monosaccharide compositions, and anti-ulcerative colitis ability to protect Caco-2 cells from lipopolysaccharide (LPS)-induced inflammation.					
34591399	2	45	theme	-induced	500:507	arg1	inflammation					509:520	lipopolysaccharide (LPS)-induced inflammation	476:520	lipopolysaccharide (LPS)-induced inflammation	476:520	This study assessed their chemical and physical properties, molecular weight, monosaccharide compositions, and anti-ulcerative colitis ability to protect Caco-2 cells from lipopolysaccharide (LPS)-induced inflammation.					
34591399	1	46	attach	isolated	254:261	arg2	wHEP-2					229:234	wHEP-2	229:234	wHEP-2	229:234	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	1	46	attach	isolated	254:261	arg2	wHEP-1					221:226	wHEP-1	221:226	wHEP-1	221:226	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	1	46	attach	isolated	254:261	arg2	wHEP-3					241:246	wHEP-3	241:246	wHEP-3	241:246	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	1	46	attach	isolated	254:261	arg2	polysaccharides					204:218	Three major polysaccharides	192:218	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3)	192:247	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
34591399	1	46	attach	isolated	254:261	arg1	mycelium					272:279	the mycelium	268:279	the mycelium of Hericium erinaceus	268:301	Three major polysaccharides (wHEP-1, wHEP-2, and wHEP-3) were isolated from the mycelium of Hericium erinaceus.					
32400829	6	0	theme	gut	1337:1339	arg1	microbiota					1341:1350	the gut microbiota and mucosal barrier function	1333:1379	microbiota	1341:1350	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	3	1	theme	AF12	708:711	arg1	abundance					662:670	the relative abundance	649:670	the relative abundance of Parabacteroides, Sutterella, and AF12	649:711	At the genus level, GPOP-1 significantly increased the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium and decreased the relative abundance of Parabacteroides, Sutterella, and AF12.					
32400829	4	2	theme	microbiota	860:869	arg1	function					840:847	the metabolic function	826:847	the metabolic function of the gut microbiota	826:869	The metabolic pathway differences according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that the metabolic function of the gut microbiota could be significantly improved by GPOP-1.					
32400829	1	3	theme	study	160:164	arg1	purpose					144:150	The purpose	140:150	The purpose of this study	140:164	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	0	4	from	effects	11:17	arg1	red					56:58	red	56:58	red	56:58	Beneficial effects of sulfated polysaccharides from the red seaweed Gelidium pacificum Okamura on mice with antibiotic-associated diarrhea.					
32400829	4	5	theme	gut	856:858	arg1	microbiota					860:869	the gut microbiota	852:869	the gut microbiota	852:869	The metabolic pathway differences according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that the metabolic function of the gut microbiota could be significantly improved by GPOP-1.					
32400829	2	6	theme	natural	362:368	arg1	group					379:383	the natural recovery group	358:383	the natural recovery group	358:383	Compared with the natural recovery group, GPOP-1 increased the richness and diversity of the gut microbiome, as well as altered the composition of the gut microbiota.					
32400829	5	7	theme	butyrates	1180:1188	arg1	contents					1145:1152	the contents	1141:1152	the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs)	1141:1231	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	8	theme	inflammatory	970:981	arg1	cytokines					983:991	inflammatory cytokines	970:991	inflammatory cytokines	970:991	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	8	theme	inflammatory	970:981	arg1	interleukin-2					1054:1066	interleukin-2	1054:1066	interleukin-2 (IL-2)	1054:1073	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	8	theme	inflammatory	970:981	arg1	interleukin-1β					1027:1040	interleukin-1β	1027:1040	interleukin-1β (IL-1β)	1027:1048	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	8	theme	inflammatory	970:981	arg1	factor-α					1009:1016	tumor necrosis factor-α	994:1016	tumor necrosis factor-α (TNF-α)	994:1024	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	1	9	theme	beneficial	274:283	arg1	effects					285:291	beneficial effects	274:291	beneficial effects	274:291	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	0	10	from	red	56:58	arg1	effects					11:17	Beneficial effects	0:17	Beneficial effects of sulfated polysaccharides from the red	0:58	Beneficial effects of sulfated polysaccharides from the red seaweed Gelidium pacificum Okamura on mice with antibiotic-associated diarrhea.					
32400829	0	10	from	red	56:58	arg1	polysaccharides					31:45	sulfated polysaccharides	22:45	sulfated polysaccharides from the red	22:58	Beneficial effects of sulfated polysaccharides from the red seaweed Gelidium pacificum Okamura on mice with antibiotic-associated diarrhea.					
32400829	5	11	theme	cytokines	983:991	arg1	concentrations					952:965	the concentrations	948:965	the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2)	948:1073	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	2	12	theme	recovery	370:377	arg1	group					379:383	the natural recovery group	358:383	the natural recovery group	358:383	Compared with the natural recovery group, GPOP-1 increased the richness and diversity of the gut microbiome, as well as altered the composition of the gut microbiota.					
32400829	0	13	theme	antibiotic-associated	108:128	arg1	diarrhea					130:137	antibiotic-associated diarrhea	108:137	antibiotic-associated diarrhea	108:137	Beneficial effects of sulfated polysaccharides from the red seaweed Gelidium pacificum Okamura on mice with antibiotic-associated diarrhea.					
32400829	3	14	theme	genus	518:522	arg1	level					524:528	the genus level	514:528	the genus level	514:528	At the genus level, GPOP-1 significantly increased the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium and decreased the relative abundance of Parabacteroides, Sutterella, and AF12.					
32400829	6	15	with	mice	1290:1293	arg1	AAD					1300:1302	AAD	1300:1302	AAD	1300:1302	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	2	16	theme	microbiota	499:508	arg1	composition					476:486	the composition	472:486	the composition of the gut microbiota	472:508	Compared with the natural recovery group, GPOP-1 increased the richness and diversity of the gut microbiome, as well as altered the composition of the gut microbiota.					
32400829	5	17	theme	pathological	1091:1102	arg1	features					1104:1111	the pathological features	1087:1111	the pathological features of the cecum	1087:1124	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	4	18	theme	metabolic	830:838	arg1	function					840:847	the metabolic function	826:847	the metabolic function of the gut microbiota	826:869	The metabolic pathway differences according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that the metabolic function of the gut microbiota could be significantly improved by GPOP-1.					
32400829	5	19	theme	tumor	994:998	arg1	cytokines					983:991	inflammatory cytokines	970:991	inflammatory cytokines	970:991	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	19	theme	tumor	994:998	arg1	TNF-α					1019:1023	TNF-α	1019:1023	TNF-α	1019:1023	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	19	theme	tumor	994:998	arg1	factor-α					1009:1016	tumor necrosis factor-α	994:1016	tumor necrosis factor-α (TNF-α)	994:1024	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	20	theme	propionates	1167:1177	arg1	contents					1145:1152	the contents	1141:1152	the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs)	1141:1231	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	0	21	theme	Beneficial	0:9	arg1	effects					11:17	Beneficial effects	0:17	Beneficial effects of sulfated polysaccharides from the red	0:58	Beneficial effects of sulfated polysaccharides from the red seaweed Gelidium pacificum Okamura on mice with antibiotic-associated diarrhea.					
32400829	0	22	with	mice	98:101	arg1	diarrhea					130:137	antibiotic-associated diarrhea	108:137	antibiotic-associated diarrhea	108:137	Beneficial effects of sulfated polysaccharides from the red seaweed Gelidium pacificum Okamura on mice with antibiotic-associated diarrhea.					
32400829	3	23	theme	relative	566:573	arg1	abundance					575:583	the relative abundance	562:583	the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium	562:633	At the genus level, GPOP-1 significantly increased the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium and decreased the relative abundance of Parabacteroides, Sutterella, and AF12.					
32400829	6	24	theme	SCFAs	1494:1498	arg1	content					1483:1489	the content	1479:1489	the content of SCFAs	1479:1498	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	1	25	theme	Gelidium	193:200	arg1	polysaccharides					220:234	Gelidium pacificum Okamura polysaccharides	193:234	Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1)	193:268	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	0	26	theme	sulfated	22:29	arg1	polysaccharides					31:45	sulfated polysaccharides	22:45	sulfated polysaccharides from the red	22:58	Beneficial effects of sulfated polysaccharides from the red seaweed Gelidium pacificum Okamura on mice with antibiotic-associated diarrhea.					
32400829	2	27	dep	richness	407:414	arg1	the					403:405	the	403:405	the	403:405	Compared with the natural recovery group, GPOP-1 increased the richness and diversity of the gut microbiome, as well as altered the composition of the gut microbiota.					
32400829	1	28	with	mice	296:299	arg1	AAD					338:340	AAD	338:340	AAD	338:340	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	1	28	with	mice	296:299	arg1	diarrhea					328:335	antibiotic-associated diarrhea	306:335	antibiotic-associated diarrhea (AAD)	306:341	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	1	29	theme	pacificum	202:210	arg1	polysaccharides					220:234	Gelidium pacificum Okamura polysaccharides	193:234	Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1)	193:268	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	2	30	theme	gut	437:439	arg1	microbiome					441:450	the gut microbiome	433:450	the gut microbiome	433:450	Compared with the natural recovery group, GPOP-1 increased the richness and diversity of the gut microbiome, as well as altered the composition of the gut microbiota.					
32400829	1	31	theme	Okamura	212:218	arg1	polysaccharides					220:234	Gelidium pacificum Okamura polysaccharides	193:234	Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1)	193:268	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	1	32	theme	antibiotic-associated	306:326	arg1	AAD					338:340	AAD	338:340	AAD	338:340	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	1	32	theme	antibiotic-associated	306:326	arg1	diarrhea					328:335	antibiotic-associated diarrhea	306:335	antibiotic-associated diarrhea (AAD)	306:341	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	3	33	theme	relative	653:660	arg1	abundance					662:670	the relative abundance	649:670	the relative abundance of Parabacteroides, Sutterella, and AF12	649:711	At the genus level, GPOP-1 significantly increased the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium and decreased the relative abundance of Parabacteroides, Sutterella, and AF12.					
32400829	3	34	theme	Bacteroides	588:598	arg1	abundance					575:583	the relative abundance	562:583	the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium	562:633	At the genus level, GPOP-1 significantly increased the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium and decreased the relative abundance of Parabacteroides, Sutterella, and AF12.					
32400829	0	35	theme	polysaccharides	31:45	arg1	effects					11:17	Beneficial effects	0:17	Beneficial effects of sulfated polysaccharides from the red	0:58	Beneficial effects of sulfated polysaccharides from the red seaweed Gelidium pacificum Okamura on mice with antibiotic-associated diarrhea.					
32400829	6	36	theme	function	1372:1379	arg1	recovery					1321:1328	the recovery	1317:1328	the recovery of the gut microbiota and mucosal barrier function	1317:1379	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	2	37	theme	microbiome	441:450	arg1	diversity					420:428	diversity	420:428	diversity	420:428	Compared with the natural recovery group, GPOP-1 increased the richness and diversity of the gut microbiome, as well as altered the composition of the gut microbiota.					
32400829	2	37	theme	microbiome	441:450	arg1	richness					407:414	richness	407:414	richness	407:414	Compared with the natural recovery group, GPOP-1 increased the richness and diversity of the gut microbiome, as well as altered the composition of the gut microbiota.					
32400829	6	38	theme	barrier	1364:1370	arg1	function					1372:1379	the gut microbiota and mucosal barrier function	1333:1379	function	1372:1379	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	5	39	theme	acetates	1157:1164	arg1	contents					1145:1152	the contents	1141:1152	the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs)	1141:1231	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	6	40	theme	beneficial	1268:1277	arg1	effects					1279:1285	beneficial effects	1268:1285	beneficial effects on mice with AAD	1268:1302	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	5	41	theme	cecum	1120:1124	arg1	features					1104:1111	the pathological features	1087:1111	the pathological features of the cecum	1087:1124	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	6	42	theme	mucosal	1356:1362	arg1	function					1372:1379	the gut microbiota and mucosal barrier function	1333:1379	function	1372:1379	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	3	43	theme	Parabacteroides	675:689	arg1	abundance					662:670	the relative abundance	649:670	the relative abundance of Parabacteroides, Sutterella, and AF12	649:711	At the genus level, GPOP-1 significantly increased the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium and decreased the relative abundance of Parabacteroides, Sutterella, and AF12.					
32400829	6	44	from	effects	1279:1285	arg1	mice					1290:1293	mice	1290:1293	mice with AAD	1290:1302	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	3	45	theme	Sutterella	692:701	arg1	abundance					662:670	the relative abundance	649:670	the relative abundance of Parabacteroides, Sutterella, and AF12	649:711	At the genus level, GPOP-1 significantly increased the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium and decreased the relative abundance of Parabacteroides, Sutterella, and AF12.					
32400829	5	46	theme	necrosis	1000:1007	arg1	cytokines					983:991	inflammatory cytokines	970:991	inflammatory cytokines	970:991	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	46	theme	necrosis	1000:1007	arg1	TNF-α					1019:1023	TNF-α	1019:1023	TNF-α	1019:1023	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	46	theme	necrosis	1000:1007	arg1	factor-α					1009:1016	tumor necrosis factor-α	994:1016	tumor necrosis factor-α (TNF-α)	994:1024	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	1	47	dep	polysaccharides	220:234	arg1	GPOP-1					262:267	GPOP-1	262:267	GPOP-1	262:267	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	1	47	dep	polysaccharides	220:234	arg1	polysaccharide					246:259	sulfated polysaccharide	237:259	sulfated polysaccharide	237:259	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	4	48	theme	pathway	728:734	arg1	differences					736:746	The metabolic pathway differences	714:746	The metabolic pathway differences according to the Kyoto Encyclopedia of Genes and Genomes (KEGG)	714:810	The metabolic pathway differences according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that the metabolic function of the gut microbiota could be significantly improved by GPOP-1.					
32400829	3	49	theme	Oscillospira	601:612	arg1	abundance					575:583	the relative abundance	562:583	the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium	562:633	At the genus level, GPOP-1 significantly increased the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium and decreased the relative abundance of Parabacteroides, Sutterella, and AF12.					
32400829	1	50	theme	sulfated	237:244	arg1	GPOP-1					262:267	GPOP-1	262:267	GPOP-1	262:267	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	1	50	theme	sulfated	237:244	arg1	polysaccharide					246:259	sulfated polysaccharide	237:259	sulfated polysaccharide	237:259	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	3	51	theme	Bifidobacterium	619:633	arg1	abundance					575:583	the relative abundance	562:583	the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium	562:633	At the genus level, GPOP-1 significantly increased the relative abundance of Bacteroides, Oscillospira, and Bifidobacterium and decreased the relative abundance of Parabacteroides, Sutterella, and AF12.					
32400829	4	52	theme	metabolic	718:726	arg1	differences					736:746	The metabolic pathway differences	714:746	The metabolic pathway differences according to the Kyoto Encyclopedia of Genes and Genomes (KEGG)	714:810	The metabolic pathway differences according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that the metabolic function of the gut microbiota could be significantly improved by GPOP-1.					
32400829	6	53	theme	cytokines	1455:1463	arg1	levels					1432:1437	the levels	1428:1437	the levels of inflammatory cytokines	1428:1463	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	5	54	theme	short-chain	1201:1211	arg1	SCFAs					1226:1230	SCFAs	1226:1230	SCFAs	1226:1230	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	54	theme	short-chain	1201:1211	arg1	acids					1219:1223	total short-chain fatty acids	1195:1223	total short-chain fatty acids (SCFAs)	1195:1231	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	2	55	theme	gut	495:497	arg1	microbiota					499:508	the gut microbiota	491:508	the gut microbiota	491:508	Compared with the natural recovery group, GPOP-1 increased the richness and diversity of the gut microbiome, as well as altered the composition of the gut microbiota.					
32400829	6	56	theme	metabolic	1392:1400	arg1	disorders					1402:1410	metabolic disorders	1392:1410	metabolic disorders	1392:1410	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	1	57	contain	had	270:272	arg2	effects					285:291	beneficial effects	274:291	beneficial effects	274:291	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	1	57	contain	had	270:272	arg1	polysaccharides					220:234	Gelidium pacificum Okamura polysaccharides	193:234	Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1)	193:268	The purpose of this study was to investigate whether Gelidium pacificum Okamura polysaccharides (sulfated polysaccharide, GPOP-1) had beneficial effects on mice with antibiotic-associated diarrhea (AAD).					
32400829	6	58	theme	inflammatory	1442:1453	arg1	cytokines					1455:1463	inflammatory cytokines	1442:1463	inflammatory cytokines	1442:1463	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	5	59	theme	fatty	1213:1217	arg1	SCFAs					1226:1230	SCFAs	1226:1230	SCFAs	1226:1230	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	59	theme	fatty	1213:1217	arg1	acids					1219:1223	total short-chain fatty acids	1195:1223	total short-chain fatty acids (SCFAs)	1195:1231	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	6	60	theme	microbiota	1341:1350	arg1	recovery					1321:1328	the recovery	1317:1328	the recovery of the gut microbiota and mucosal barrier function	1317:1379	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	5	61	theme	total	1195:1199	arg1	SCFAs					1226:1230	SCFAs	1226:1230	SCFAs	1226:1230	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	5	61	theme	total	1195:1199	arg1	acids					1219:1223	total short-chain fatty acids	1195:1223	total short-chain fatty acids (SCFAs)	1195:1231	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
32400829	6	62	contain	had	1264:1266	arg2	effects					1279:1285	beneficial effects	1268:1285	beneficial effects on mice with AAD	1268:1302	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	6	62	contain	had	1264:1266	arg1	GPOP-1					1257:1262	GPOP-1	1257:1262	GPOP-1	1257:1262	Results indicated that GPOP-1 had beneficial effects on mice with AAD by promoting the recovery of the gut microbiota and mucosal barrier function, reversing metabolic disorders, downregulating the levels of inflammatory cytokines and improving the content of SCFAs.					
32400829	5	63	theme	acids	1219:1223	arg1	contents					1145:1152	the contents	1141:1152	the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs)	1141:1231	Furthermore, GPOP-1 downregulated the concentrations of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-2 (IL-2), alleviated the pathological features of the cecum, and increased the contents of acetates, propionates, butyrates, and total short-chain fatty acids (SCFAs).					
33892026	7	0	theme	coating	1008:1014	arg1	terms					999:1003	terms	999:1003	terms of coating or films	999:1023	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	7	1	theme	sterile	1194:1200	arg1	water					1202:1206	sterile water	1194:1206	sterile water	1194:1206	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	1	2	theme	emerging	146:153	arg1	products					166:173	the emerging commercial products	142:173	the emerging commercial products	142:173	Due to the convenience, fresh-cut vegetables or fruits as the emerging commercial products have attracted much attention in recent years.					
33892026	4	3	theme	PAX	492:494	arg1	film					496:499	PAX film	492:499	PAX film based on the shearing viscosity	492:531	The optimum concentrations for pectin and SA in PAX film based on the shearing viscosity were 6 g/L and 5 g/L, respectively.					
33892026	8	4	theme	PAXO	1256:1259	arg1	film					1261:1264	the as-prepared PAXO film	1240:1264	the as-prepared PAXO film	1240:1264	All the results indicated that the as-prepared PAXO film or PAXO solution could be good candidates in packaging preservation.					
33892026	8	4	theme	PAXO	1256:1259	arg1	candidates					1297:1306	good candidates	1292:1306	good candidates	1292:1306	All the results indicated that the as-prepared PAXO film or PAXO solution could be good candidates in packaging preservation.					
33892026	1	5	theme	commercial	155:164	arg1	products					166:173	the emerging commercial products	142:173	the emerging commercial products	142:173	Due to the convenience, fresh-cut vegetables or fruits as the emerging commercial products have attracted much attention in recent years.					
33892026	8	6	theme	PAXO	1269:1272	arg1	solution					1274:1281	PAXO solution	1269:1281	PAXO solution	1269:1281	All the results indicated that the as-prepared PAXO film or PAXO solution could be good candidates in packaging preservation.					
33892026	5	7	theme	CaCl2	823:827	arg1	concentration					768:780	the concentration	764:780	the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2	764:827	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	6	8	from	break	862:866	arg1	%					877:877	19.02%	872:877	19.02%	872:877	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	6	8	from	break	862:866	arg1	elongation					848:857	The corresponding elongation	830:857	The corresponding elongation at break	830:866	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	5	9	theme	glycerol	802:809	arg1	concentration					768:780	the concentration	764:780	the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2	764:827	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	5	10	theme	g/L	798:800	arg1	glycerol					802:809	18 g/L glycerol	795:809	18 g/L glycerol	795:809	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	3	11	theme	edible	332:337	arg1	PAX					355:357	PAX	355:357	PAX	355:357	In this study, one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG) was well developed.					
33892026	3	11	theme	edible	332:337	arg1	film					349:352	edible composite film	332:352	edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG)	332:422	In this study, one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG) was well developed.					
33892026	6	12	theme	corresponding	834:846	arg1	%					877:877	19.02%	872:877	19.02%	872:877	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	6	12	theme	corresponding	834:846	arg1	elongation					848:857	The corresponding elongation	830:857	The corresponding elongation at break	830:866	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	3	13	theme	novel	318:322	arg1	kind					324:327	one novel kind	314:327	one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG)	314:422	In this study, one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG) was well developed.					
33892026	5	14	theme	optimized	694:702	arg1	film					715:718	the optimized PAX (PAXO) film	690:718	the optimized PAX (PAXO) film	690:718	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	7	15	theme	food	1110:1113	arg1	preservation					1115:1126	food preservation	1110:1126	food preservation with the order	1110:1141	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	6	16	theme	transmission	899:910	arg1	rate					912:915	the water vapor transmission rate	883:915	the water vapor transmission rate	883:915	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	6	16	theme	transmission	899:910	arg1	g/					951:952	18.12 × 10-11 g/	937:952	18.12 × 10-11 g/(m2·s·pa)	937:961	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	5	17	theme	surface	633:639	arg1	methodology					641:651	the response surface methodology	620:651	the response surface methodology	620:651	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	5	18	theme	tensile	669:675	arg1	strength					677:684	the tensile strength	665:684	the tensile strength for the optimized PAX (PAXO) film	665:718	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	2	19	theme	high	261:264	arg1	quality					266:272	high quality	261:272	high quality	261:272	However, the preservation of food with high quality remains a big challenge.					
33892026	8	20	from	candidates	1297:1306	arg1	preservation					1321:1332	packaging preservation	1311:1332	packaging preservation	1311:1332	All the results indicated that the as-prepared PAXO film or PAXO solution could be good candidates in packaging preservation.					
33892026	5	21	theme	maximum	734:740	arg1	value					742:746	the maximum value	730:746	the maximum value of 29.65 MPa	730:759	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	0	22	theme	alginate/xanthan	14:29	arg1	gum					31:33	Pectin/sodium alginate/xanthan gum	0:33	Pectin/sodium alginate/xanthan gum	0:33	Pectin/sodium alginate/xanthan gum edible composite films as the fresh-cut package.					
33892026	8	23	theme	good	1292:1295	arg1	film					1261:1264	the as-prepared PAXO film	1240:1264	the as-prepared PAXO film	1240:1264	All the results indicated that the as-prepared PAXO film or PAXO solution could be good candidates in packaging preservation.					
33892026	8	23	theme	good	1292:1295	arg1	candidates					1297:1306	good candidates	1292:1306	good candidates	1292:1306	All the results indicated that the as-prepared PAXO film or PAXO solution could be good candidates in packaging preservation.					
33892026	7	24	theme	fresh-cut	1043:1051	arg1	potatoes					1053:1060	fresh-cut potatoes	1043:1060	fresh-cut potatoes	1043:1060	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	5	25	theme	XG	791:792	arg1	concentration					768:780	the concentration	764:780	the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2	764:827	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	6	26	theme	water	887:891	arg1	vapor					893:897	water vapor	887:897	the water vapor transmission rate	883:915	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	0	27	theme	Pectin/sodium	0:12	arg1	gum					31:33	Pectin/sodium alginate/xanthan gum	0:33	Pectin/sodium alginate/xanthan gum	0:33	Pectin/sodium alginate/xanthan gum edible composite films as the fresh-cut package.					
33892026	8	28	theme	packaging	1311:1319	arg1	preservation					1321:1332	packaging preservation	1311:1332	packaging preservation	1311:1332	All the results indicated that the as-prepared PAXO film or PAXO solution could be good candidates in packaging preservation.					
33892026	8	29	theme	as-prepared	1244:1254	arg1	film					1261:1264	the as-prepared PAXO film	1240:1264	the as-prepared PAXO film	1240:1264	All the results indicated that the as-prepared PAXO film or PAXO solution could be good candidates in packaging preservation.					
33892026	8	29	theme	as-prepared	1244:1254	arg1	candidates					1297:1306	good candidates	1292:1306	good candidates	1292:1306	All the results indicated that the as-prepared PAXO film or PAXO solution could be good candidates in packaging preservation.					
33892026	3	30	theme	sodium	381:386	arg1	alginate					388:395	sodium alginate	381:395	sodium alginate (SA)	381:400	In this study, one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG) was well developed.					
33892026	3	30	theme	sodium	381:386	arg1	SA					398:399	SA	398:399	SA	398:399	In this study, one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG) was well developed.					
33892026	3	31	theme	composite	339:347	arg1	PAX					355:357	PAX	355:357	PAX	355:357	In this study, one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG) was well developed.					
33892026	3	31	theme	composite	339:347	arg1	film					349:352	edible composite film	332:352	edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG)	332:422	In this study, one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG) was well developed.					
33892026	5	32	theme	PAX	704:706	arg1	film					715:718	the optimized PAX (PAXO) film	690:718	the optimized PAX (PAXO) film	690:718	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	7	33	theme	CaCl2	1159:1163	arg1	PAXO					1167:1170	CaCl2 ≈ PAXO	1159:1170	CaCl2 ≈ PAXO	1159:1170	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	5	34	theme	18	795:796	arg1	g/L					798:800	g/L	798:800	g/L	798:800	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	3	35	theme	film	349:352	arg1	kind					324:327	one novel kind	314:327	one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG)	314:422	In this study, one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG) was well developed.					
33892026	7	36	from	nanocomposites	981:994	arg1	terms					999:1003	terms	999:1003	terms of coating or films	999:1023	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	2	37	theme	big	284:286	arg1	challenge					288:296	a big challenge	282:296	a big challenge	282:296	However, the preservation of food with high quality remains a big challenge.					
33892026	4	38	from	concentrations	456:469	arg1	film					496:499	PAX film	492:499	PAX film based on the shearing viscosity	492:531	The optimum concentrations for pectin and SA in PAX film based on the shearing viscosity were 6 g/L and 5 g/L, respectively.					
33892026	5	39	theme	experimental	594:605	arg1	results					607:613	the experimental results	590:613	the experimental results from the response surface methodology	590:651	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	7	40	theme	≈	1165:1165	arg1	PAXO					1167:1170	CaCl2 ≈ PAXO	1159:1170	CaCl2 ≈ PAXO	1159:1170	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	1	41	theme	much	190:193	arg1	attention					195:203	much attention	190:203	much attention	190:203	Due to the convenience, fresh-cut vegetables or fruits as the emerging commercial products have attracted much attention in recent years.					
33892026	1	42	theme	recent	208:213	arg1	years					215:219	recent years	208:219	recent years	208:219	Due to the convenience, fresh-cut vegetables or fruits as the emerging commercial products have attracted much attention in recent years.					
33892026	0	43	theme	composite	42:50	arg1	films					52:56	edible composite films	35:56	edible composite films	35:56	Pectin/sodium alginate/xanthan gum edible composite films as the fresh-cut package.					
33892026	0	44	theme	edible	35:40	arg1	films					52:56	edible composite films	35:56	edible composite films	35:56	Pectin/sodium alginate/xanthan gum edible composite films as the fresh-cut package.					
33892026	7	45	used	used	1030:1033	arg2	nanocomposites					981:994	the nanocomposites	977:994	the nanocomposites in terms of coating or films	977:1023	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	2	46	with	preservation	235:246	arg1	quality					266:272	high quality	261:272	high quality	261:272	However, the preservation of food with high quality remains a big challenge.					
33892026	6	47	theme	18.12	937:941	arg1	m2·s·pa					954:960	m2·s·pa	954:960	m2·s·pa	954:960	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	6	47	theme	18.12	937:941	arg1	g/					951:952	18.12 × 10-11 g/	937:952	18.12 × 10-11 g/(m2·s·pa)	937:961	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	6	47	theme	18.12	937:941	arg1	rate					912:915	the water vapor transmission rate	883:915	the water vapor transmission rate	883:915	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	1	48	theme	fresh-cut	108:116	arg1	vegetables					118:127	vegetables	118:127	vegetables	118:127	Due to the convenience, fresh-cut vegetables or fruits as the emerging commercial products have attracted much attention in recent years.					
33892026	7	49	theme	PAXO	1182:1185	arg1	film					1187:1190	> PAXO film	1180:1190	> PAXO film	1180:1190	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	7	50	with	preservation	1115:1126	arg1	order					1137:1141	the order	1133:1141	the order	1133:1141	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	7	51	theme	>	1180:1180	arg1	film					1187:1190	> PAXO film	1180:1190	> PAXO film	1180:1190	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	6	52	theme	×	943:943	arg1	m2·s·pa					954:960	m2·s·pa	954:960	m2·s·pa	954:960	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	6	52	theme	×	943:943	arg1	g/					951:952	18.12 × 10-11 g/	937:952	18.12 × 10-11 g/(m2·s·pa)	937:961	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	6	52	theme	×	943:943	arg1	rate					912:915	the water vapor transmission rate	883:915	the water vapor transmission rate	883:915	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	4	53	theme	optimum	448:454	arg1	g/L					540:542	6 g/L and 5 g/L	538:552	g/L	540:542	The optimum concentrations for pectin and SA in PAX film based on the shearing viscosity were 6 g/L and 5 g/L, respectively.					
33892026	4	53	theme	optimum	448:454	arg1	concentrations					456:469	The optimum concentrations	444:469	The optimum concentrations for pectin and SA in PAX film based on the shearing viscosity	444:531	The optimum concentrations for pectin and SA in PAX film based on the shearing viscosity were 6 g/L and 5 g/L, respectively.					
33892026	7	54	theme	PAXO	1144:1147	arg1	coating					1149:1155	PAXO coating	1144:1155	PAXO coating	1144:1155	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	5	55	theme	g/L	819:821	arg1	CaCl2					823:827	20 g/L CaCl2	816:827	20 g/L CaCl2	816:827	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	3	56	theme	xanthan	407:413	arg1	XG					420:421	XG	420:421	XG	420:421	In this study, one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG) was well developed.					
33892026	3	56	theme	xanthan	407:413	arg1	gum					415:417	xanthan gum	407:417	xanthan gum (XG)	407:422	In this study, one novel kind of edible composite film (PAX) consisted of pectin, sodium alginate (SA), and xanthan gum (XG) was well developed.					
33892026	5	57	theme	response	624:631	arg1	methodology					641:651	the response surface methodology	620:651	the response surface methodology	620:651	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	5	58	theme	20	816:817	arg1	g/L					819:821	g/L	819:821	g/L	819:821	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	5	59	from	methodology	641:651	arg1	results					607:613	the experimental results	590:613	the experimental results from the response surface methodology	590:651	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	2	60	theme	food	251:254	arg1	preservation					235:246	the preservation	231:246	the preservation of food with high quality	231:272	However, the preservation of food with high quality remains a big challenge.					
33892026	5	61	theme	g/L	787:789	arg1	XG					791:792	4 g/L XG	785:792	4 g/L XG	785:792	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	5	62	theme	4	785:785	arg1	g/L					787:789	g/L	787:789	g/L	787:789	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	7	63	theme	films	1019:1023	arg1	terms					999:1003	terms	999:1003	terms of coating or films	999:1023	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	7	64	theme	different	1084:1092	arg1	efficiencies					1094:1105	different efficiencies	1084:1105	different efficiencies	1084:1105	Furthermore, the nanocomposites in terms of coating or films were used to keep fresh-cut potatoes, where they exhibited different efficiencies in food preservation with the order: PAXO coating + CaCl2 ≈ PAXO coating > PAXO film > sterile water.					
33892026	6	65	theme	vapor	893:897	arg1	rate					912:915	the water vapor transmission rate	883:915	the water vapor transmission rate	883:915	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	6	65	theme	vapor	893:897	arg1	g/					951:952	18.12 × 10-11 g/	937:952	18.12 × 10-11 g/(m2·s·pa)	937:961	The corresponding elongation at break was 19.02% and the water vapor transmission rate was evaluated to be 18.12 × 10-11 g/(m2·s·pa).					
33892026	0	66	theme	fresh-cut	65:73	arg1	package					75:81	the fresh-cut package	61:81	the fresh-cut package	61:81	Pectin/sodium alginate/xanthan gum edible composite films as the fresh-cut package.					
33892026	5	67	theme	PAXO	709:712	arg1	film					715:718	the optimized PAX (PAXO) film	690:718	the optimized PAX (PAXO) film	690:718	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	5	68	theme	MPa	757:759	arg1	value					742:746	the maximum value	730:746	the maximum value of 29.65 MPa	730:759	Upon this condition, the experimental results from the response surface methodology showed that the tensile strength for the optimized PAX (PAXO) film can reach the maximum value of 29.65 MPa at the concentration of 4 g/L XG, 18 g/L glycerol, and 20 g/L CaCl2.					
33892026	4	69	theme	shearing	514:521	arg1	viscosity					523:531	the shearing viscosity	510:531	the shearing viscosity	510:531	The optimum concentrations for pectin and SA in PAX film based on the shearing viscosity were 6 g/L and 5 g/L, respectively.					
32919571	4	0	theme	molecular	906:914	arg1	weight					916:921	decreased average molecular weight	888:921	decreased average molecular weight	888:921	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	1	1	theme	franchetii	188:197	arg1	NFP					206:208	NFP	206:208	NFP	206:208	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	1	1	theme	franchetii	188:197	arg1	Boiss					199:203	Notopterygium franchetii Boiss	174:203	Notopterygium franchetii Boiss (NFP)	174:209	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	4	2	theme	average	898:904	arg1	weight					916:921	decreased average molecular weight	888:921	decreased average molecular weight	888:921	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	6	3	used	used	1193:1196	arg2	NFP-MAATPS					1161:1170	purified NFP-MAATPS	1152:1170	purified NFP-MAATPS	1152:1170	Our results suggest that purified NFP-MAATPS could be potentially used in complementary medicines or functional foods.					
32919571	1	4	theme	response	269:276	arg1	RSM					299:301	RSM	299:301	RSM	299:301	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	1	4	theme	response	269:276	arg1	methodology					286:296	response surface methodology	269:296	response surface methodology (RSM)	269:302	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	2	5	theme	extraction	468:477	arg1	time					479:482	extraction time	468:482	extraction time of 15 min	468:492	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	4	6	theme	extracted	672:680	arg1	NFP-MAATPS					699:708	NFP-MAATPS	699:708	NFP-MAATPS	699:708	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	4	6	theme	extracted	672:680	arg1	polysaccharides					682:696	extracted polysaccharides	672:696	extracted polysaccharides (NFP-MAATPS) under the optimal conditions	672:738	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	1	7	from	Boiss	199:203	arg1	polysaccharides					153:167	polysaccharides	153:167	polysaccharides from Notopterygium franchetii Boiss (NFP)	153:209	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	1	7	from	Boiss	199:203	arg1	extraction					139:148	the extraction	135:148	the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP)	135:209	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	4	8	theme	physicochemical	642:656	arg1	properties					658:667	the physicochemical properties	638:667	the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions	638:738	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	1	9	theme	surface	278:284	arg1	RSM					299:301	RSM	299:301	RSM	299:301	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	1	9	theme	surface	278:284	arg1	methodology					286:296	response surface methodology	269:296	response surface methodology (RSM)	269:302	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	5	10	from	activity	1031:1038	arg1	embryos					1105:1111	zebrafish embryos	1095:1111	zebrafish embryos	1095:1111	Moreover, NFP-MAATPS exhibited stronger antioxidant activity in vitro and more potent anti-inflammatory activity in zebrafish embryos than NFP-HWE.					
32919571	2	11	theme	MAATPS	365:370	arg1	conditions					351:360	the optimum conditions	339:360	the optimum conditions of MAATPS	339:370	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	3	12	dep	YP	582:583	arg1	recovery					590:597	the recovery	586:597	the recovery of proteins	586:609	Under such condition, YS (the recovery of polysaccharides) was 80.57 % with 90.40 % of YP (the recovery of proteins).					
32919571	6	13	theme	functional	1228:1237	arg1	foods					1239:1243	functional foods	1228:1243	functional foods	1228:1243	Our results suggest that purified NFP-MAATPS could be potentially used in complementary medicines or functional foods.					
32919571	2	14	theme	%	417:417	arg1	time					479:482	extraction time	468:482	extraction time of 15 min	468:492	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	2	14	theme	%	417:417	arg1	concentration					397:409	ethanol concentration	389:409	ethanol concentration of 32 % (w/w)	389:423	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	2	14	theme	%	417:417	arg1	concentration					436:448	(NH4)2SO4 concentration	426:448	(NH4)2SO4 concentration of 24 % (w/w)	426:462	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	4	15	theme	extraction	865:874	arg1	efficiency					876:885	higher extraction efficiency	858:885	higher extraction efficiency	858:885	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	3	16	theme	proteins	602:609	arg1	recovery					590:597	the recovery	586:597	the recovery of proteins	586:609	Under such condition, YS (the recovery of polysaccharides) was 80.57 % with 90.40 % of YP (the recovery of proteins).					
32919571	0	17	dep	Boiss	116:120	arg1	anti-inflammation					49:65	anti-inflammation	49:65	anti-inflammation	49:65	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	0	17	dep	Boiss	116:120	arg1	extraction					10:19	Efficient extraction	0:19	Efficient extraction	0:19	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	0	17	dep	Boiss	116:120	arg1	activities					34:43	antioxidant activities	22:43	antioxidant activities	22:43	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	2	18	theme	ethanol	389:395	arg1	concentration					397:409	ethanol concentration	389:409	ethanol concentration of 32 % (w/w)	389:423	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	3	19	theme	such	501:504	arg1	condition					506:514	such condition	501:514	such condition	501:514	Under such condition, YS (the recovery of polysaccharides) was 80.57 % with 90.40 % of YP (the recovery of proteins).					
32919571	0	20	theme	Efficient	0:8	arg1	extraction					10:19	Efficient extraction	0:19	Efficient extraction	0:19	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	6	21	theme	complementary	1201:1213	arg1	medicines					1215:1223	complementary medicines	1201:1223	complementary medicines	1201:1223	Our results suggest that purified NFP-MAATPS could be potentially used in complementary medicines or functional foods.					
32919571	0	22	theme	antioxidant	22:32	arg1	activities					34:43	antioxidant activities	22:43	antioxidant activities	22:43	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	2	23	theme	min	490:492	arg1	time					479:482	extraction time	468:482	extraction time of 15 min	468:492	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	2	23	theme	min	490:492	arg1	concentration					397:409	ethanol concentration	389:409	ethanol concentration of 32 % (w/w)	389:423	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	2	23	theme	min	490:492	arg1	concentration					436:448	(NH4)2SO4 concentration	426:448	(NH4)2SO4 concentration of 24 % (w/w)	426:462	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	1	24	from	extraction	139:148	arg1	NFP					206:208	NFP	206:208	NFP	206:208	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	1	24	from	extraction	139:148	arg1	Boiss					199:203	Notopterygium franchetii Boiss	174:203	Notopterygium franchetii Boiss (NFP)	174:209	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	1	25	theme	microwave-assisted	212:229	arg1	MAATPS					257:262	microwave-assisted aqueous two phase system (MAATPS)	212:263	microwave-assisted aqueous two phase system (MAATPS)	212:263	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	4	26	theme	mole	966:969	arg1	ratios					971:976	mole ratios	966:976	mole ratios	966:976	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	5	27	dep	potent	1058:1063	arg1	anti-inflammatory					1065:1081	anti-inflammatory	1065:1081	anti-inflammatory	1065:1081	Moreover, NFP-MAATPS exhibited stronger antioxidant activity in vitro and more potent anti-inflammatory activity in zebrafish embryos than NFP-HWE.					
32919571	0	28	from	franchetii	105:114	arg1	anti-inflammation					49:65	anti-inflammation	49:65	anti-inflammation	49:65	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	0	28	from	franchetii	105:114	arg1	extraction					10:19	Efficient extraction	0:19	Efficient extraction	0:19	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	0	28	from	franchetii	105:114	arg1	activities					34:43	antioxidant activities	22:43	antioxidant activities	22:43	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	3	29	with	%	564:564	arg1	%					577:577	90.40 %	571:577	90.40 % of YP (the recovery of proteins)	571:610	Under such condition, YS (the recovery of polysaccharides) was 80.57 % with 90.40 % of YP (the recovery of proteins).					
32919571	3	29	with	%	564:564	arg1	YP					582:583	YP	582:583	YP (the recovery of proteins)	582:610	Under such condition, YS (the recovery of polysaccharides) was 80.57 % with 90.40 % of YP (the recovery of proteins).					
32919571	1	30	theme	aqueous	231:237	arg1	MAATPS					257:262	microwave-assisted aqueous two phase system (MAATPS)	212:263	microwave-assisted aqueous two phase system (MAATPS)	212:263	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	5	31	from	activity	1083:1090	arg1	embryos					1105:1111	zebrafish embryos	1095:1111	zebrafish embryos	1095:1111	Moreover, NFP-MAATPS exhibited stronger antioxidant activity in vitro and more potent anti-inflammatory activity in zebrafish embryos than NFP-HWE.					
32919571	5	32	theme	stronger	1010:1017	arg1	activity					1031:1038	stronger antioxidant activity	1010:1038	stronger antioxidant activity in vitro	1010:1047	Moreover, NFP-MAATPS exhibited stronger antioxidant activity in vitro and more potent anti-inflammatory activity in zebrafish embryos than NFP-HWE.					
32919571	2	33	theme	2SO4	431:434	arg1	concentration					436:448	(NH4)2SO4 concentration	426:448	(NH4)2SO4 concentration of 24 % (w/w)	426:462	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	4	34	theme	different	924:932	arg1	compositions					949:960	different monosaccharide compositions	924:960	different monosaccharide compositions	924:960	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	5	35	theme	antioxidant	1019:1029	arg1	activity					1031:1038	stronger antioxidant activity	1010:1038	stronger antioxidant activity in vitro	1010:1047	Moreover, NFP-MAATPS exhibited stronger antioxidant activity in vitro and more potent anti-inflammatory activity in zebrafish embryos than NFP-HWE.					
32919571	1	36	theme	phase	243:247	arg1	MAATPS					257:262	microwave-assisted aqueous two phase system (MAATPS)	212:263	microwave-assisted aqueous two phase system (MAATPS)	212:263	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	4	37	theme	monosaccharide	934:947	arg1	compositions					949:960	different monosaccharide compositions	924:960	different monosaccharide compositions	924:960	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	4	38	theme	higher	858:863	arg1	efficiency					876:885	higher extraction efficiency	858:885	higher extraction efficiency	858:885	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	4	39	theme	extraction	808:817	arg1	method					819:824	traditional extraction method	796:824	traditional extraction method (NFP-HWE)	796:834	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	4	39	theme	extraction	808:817	arg1	NFP-HWE					827:833	NFP-HWE	827:833	NFP-HWE	827:833	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	2	40	theme	%	456:456	arg1	time					479:482	extraction time	468:482	extraction time of 15 min	468:492	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	2	40	theme	%	456:456	arg1	concentration					397:409	ethanol concentration	389:409	ethanol concentration of 32 % (w/w)	389:423	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	2	40	theme	%	456:456	arg1	concentration					436:448	(NH4)2SO4 concentration	426:448	(NH4)2SO4 concentration of 24 % (w/w)	426:462	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	4	41	theme	optimal	721:727	arg1	conditions					729:738	the optimal conditions	717:738	the optimal conditions	717:738	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	2	42	theme	NH4	427:429	arg1	concentration					436:448	(NH4)2SO4 concentration	426:448	(NH4)2SO4 concentration of 24 % (w/w)	426:462	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	4	43	theme	traditional	796:806	arg1	method					819:824	traditional extraction method	796:824	traditional extraction method (NFP-HWE)	796:834	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	4	43	theme	traditional	796:806	arg1	NFP-HWE					827:833	NFP-HWE	827:833	NFP-HWE	827:833	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	5	44	theme	zebrafish	1095:1103	arg1	embryos					1105:1111	zebrafish embryos	1095:1111	zebrafish embryos	1095:1111	Moreover, NFP-MAATPS exhibited stronger antioxidant activity in vitro and more potent anti-inflammatory activity in zebrafish embryos than NFP-HWE.					
32919571	2	45	theme	optimum	343:349	arg1	conditions					351:360	the optimum conditions	339:360	the optimum conditions of MAATPS	339:370	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	3	46	theme	YP	582:583	arg1	%					577:577	90.40 %	571:577	90.40 % of YP (the recovery of proteins)	571:610	Under such condition, YS (the recovery of polysaccharides) was 80.57 % with 90.40 % of YP (the recovery of proteins).					
32919571	3	46	theme	YP	582:583	arg1	YP					582:583	YP	582:583	YP (the recovery of proteins)	582:610	Under such condition, YS (the recovery of polysaccharides) was 80.57 % with 90.40 % of YP (the recovery of proteins).					
32919571	3	47	theme	polysaccharides	537:551	arg1	recovery					525:532	the recovery	521:532	the recovery of polysaccharides	521:551	Under such condition, YS (the recovery of polysaccharides) was 80.57 % with 90.40 % of YP (the recovery of proteins).					
32919571	1	48	theme	system	249:254	arg1	MAATPS					257:262	microwave-assisted aqueous two phase system (MAATPS)	212:263	microwave-assisted aqueous two phase system (MAATPS)	212:263	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	4	49	theme	properties	658:667	arg1	analysis					626:633	analysis	626:633	analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions	626:738	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	2	50	dep	follows	380:386	arg1	time					479:482	extraction time	468:482	extraction time of 15 min	468:492	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	2	50	dep	follows	380:386	arg1	concentration					397:409	ethanol concentration	389:409	ethanol concentration of 32 % (w/w)	389:423	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	2	50	dep	follows	380:386	arg1	concentration					436:448	(NH4)2SO4 concentration	426:448	(NH4)2SO4 concentration of 24 % (w/w)	426:462	Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH4)2SO4 concentration of 24 % (w/w) and extraction time of 15 min.					
32919571	1	51	theme	polysaccharides	153:167	arg1	extraction					139:148	the extraction	135:148	the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP)	135:209	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	4	52	theme	polysaccharides	682:696	arg1	properties					658:667	the physicochemical properties	638:667	the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions	638:738	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	4	53	theme	decreased	888:896	arg1	weight					916:921	decreased average molecular weight	888:921	decreased average molecular weight	888:921	In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios.					
32919571	6	54	theme	purified	1152:1159	arg1	NFP-MAATPS					1161:1170	purified NFP-MAATPS	1152:1170	purified NFP-MAATPS	1152:1170	Our results suggest that purified NFP-MAATPS could be potentially used in complementary medicines or functional foods.					
32919571	3	55	dep	YS	517:518	arg1	recovery					525:532	the recovery	521:532	the recovery of polysaccharides	521:551	Under such condition, YS (the recovery of polysaccharides) was 80.57 % with 90.40 % of YP (the recovery of proteins).					
32919571	0	56	theme	polysaccharides	70:84	arg1	anti-inflammation					49:65	anti-inflammation	49:65	anti-inflammation	49:65	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	0	56	theme	polysaccharides	70:84	arg1	extraction					10:19	Efficient extraction	0:19	Efficient extraction	0:19	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	0	56	theme	polysaccharides	70:84	arg1	activities					34:43	antioxidant activities	22:43	antioxidant activities	22:43	Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.					
32919571	5	57	theme	potent	1058:1063	arg1	activity					1083:1090	more potent anti-inflammatory activity	1053:1090	more potent anti-inflammatory activity in zebrafish embryos	1053:1111	Moreover, NFP-MAATPS exhibited stronger antioxidant activity in vitro and more potent anti-inflammatory activity in zebrafish embryos than NFP-HWE.					
32919571	1	58	theme	Notopterygium	174:186	arg1	NFP					206:208	NFP	206:208	NFP	206:208	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32919571	1	58	theme	Notopterygium	174:186	arg1	Boiss					199:203	Notopterygium franchetii Boiss	174:203	Notopterygium franchetii Boiss (NFP)	174:209	To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed.					
32088222	1	0	theme	polysaccharides	341:355	arg1	characteristics					283:297	structure characteristics	273:297	structure characteristics	273:297	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	0	theme	polysaccharides	341:355	arg1	activity					329:336	hypoglycemic and antioxidant activity	300:336	hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems	300:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	0	theme	polysaccharides	341:355	arg1	yield					244:248	the yield	240:248	the yield	240:248	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	0	theme	polysaccharides	341:355	arg1	composition					260:270	chemical composition	251:270	chemical composition	251:270	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	0	1	theme	biological	163:172	arg1	activities					174:183	biological activities	163:183	biological activities	163:183	A comparison of a polysaccharide extracted from ginger (Zingiber officinale) stems and leaves using different methods: preparation, structure characteristics, and biological activities.					
32088222	8	2	theme	effective	1536:1544	arg1	potential					1520:1528	great potential	1514:1528	great potential	1514:1528	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	8	2	theme	effective	1536:1544	arg1	strategy					1546:1553	an effective strategy	1533:1553	an effective strategy for obtaining polysaccharides from stems	1533:1594	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	1	3	from	composition	260:270	arg1	stems					398:402	ginger (Zingiber officinale Roscoe) stems	362:402	ginger (Zingiber officinale Roscoe) stems	362:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	7	4	theme	uronic	1400:1405	arg1	content					1412:1418	its abundant uronic acid content	1387:1418	its abundant uronic acid content	1387:1418	In addition, ASE-GSLP displayed better biological activities probably due to its abundant uronic acid content, higher sulfate radical, and smaller molecular weight.					
32088222	1	5	from	activity	329:336	arg1	stems					398:402	ginger (Zingiber officinale Roscoe) stems	362:402	ginger (Zingiber officinale Roscoe) stems	362:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	5	6	theme	antioxidant	888:898	arg1	activity					900:907	antioxidant activity	888:907	antioxidant activity	888:907	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	1	7	theme	ginger	362:367	arg1	stems					398:402	ginger (Zingiber officinale Roscoe) stems	362:402	ginger (Zingiber officinale Roscoe) stems	362:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	2	8	theme	solution	566:573	arg1	extraction					575:584	alkaline solution extraction	557:584	alkaline solution extraction (ASE)	557:590	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	2	8	theme	solution	566:573	arg1	ASE					587:589	ASE	587:589	ASE	587:589	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	6	9	dep	leaves	1114:1119	arg1	obtained					1121:1128	obtained	1121:1128	leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions	1114:1191	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	5	10	theme	ASE-GSLP	968:975	arg1	capacities					954:963	the biological capacities	939:963	the biological capacities of ASE-GSLP	939:975	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	5	10	theme	ASE-GSLP	968:975	arg1	superior					982:989	superior	982:989	superior	982:989	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	7	11	theme	sulfate	1428:1434	arg1	radical					1436:1442	higher sulfate radical	1421:1442	higher sulfate radical	1421:1442	In addition, ASE-GSLP displayed better biological activities probably due to its abundant uronic acid content, higher sulfate radical, and smaller molecular weight.					
32088222	1	12	theme	chemical	251:258	arg1	composition					260:270	chemical composition	251:270	chemical composition	251:270	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	2	13	theme	alkaline	557:564	arg1	extraction					575:584	alkaline solution extraction	557:584	alkaline solution extraction (ASE)	557:590	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	2	13	theme	alkaline	557:564	arg1	ASE					587:589	ASE	587:589	ASE	587:589	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	6	14	theme	main	1293:1296	arg1	structures					1298:1307	their main structures	1287:1307	their main structures	1287:1307	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	7	15	theme	smaller	1449:1455	arg1	weight					1467:1472	smaller molecular weight	1449:1472	smaller molecular weight	1449:1472	In addition, ASE-GSLP displayed better biological activities probably due to its abundant uronic acid content, higher sulfate radical, and smaller molecular weight.					
32088222	8	16	theme	remarkable	1643:1652	arg1	bioactivity					1654:1664	the remarkable bioactivity	1639:1664	the remarkable bioactivity of its products	1639:1680	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	1	17	theme	Zingiber	370:377	arg1	ginger					362:367	ginger	362:367	ginger (Zingiber officinale Roscoe) stems	362:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	17	theme	Zingiber	370:377	arg1	Roscoe					390:395	Zingiber officinale Roscoe	370:395	Zingiber officinale Roscoe	370:395	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	18	from	characteristics	283:297	arg1	stems					398:402	ginger (Zingiber officinale Roscoe) stems	362:402	ginger (Zingiber officinale Roscoe) stems	362:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	2	19	theme	enzyme-assisted	597:611	arg1	EAE					625:627	EAE	625:627	EAE	625:627	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	2	19	theme	enzyme-assisted	597:611	arg1	extraction					613:622	enzyme-assisted extraction	597:622	enzyme-assisted extraction (EAE)	597:628	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	4	20	theme	electron	741:748	arg1	microscopy					750:759	Scanning electron microscopy	732:759	Scanning electron microscopy	732:759	Scanning electron microscopy indicated that GSLP microstructures were greatly influenced by extraction method.					
32088222	7	21	theme	better	1342:1347	arg1	activities					1360:1369	better biological activities	1342:1369	better biological activities	1342:1369	In addition, ASE-GSLP displayed better biological activities probably due to its abundant uronic acid content, higher sulfate radical, and smaller molecular weight.					
32088222	1	22	from	stems	398:402	arg1	characteristics					283:297	structure characteristics	273:297	structure characteristics	273:297	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	22	from	stems	398:402	arg1	activity					329:336	hypoglycemic and antioxidant activity	300:336	hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems	300:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	22	from	stems	398:402	arg1	polysaccharides					341:355	polysaccharides	341:355	polysaccharides from ginger (Zingiber officinale Roscoe) stems	341:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	22	from	stems	398:402	arg1	yield					244:248	the yield	240:248	the yield	240:248	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	22	from	stems	398:402	arg1	composition					260:270	chemical composition	251:270	chemical composition	251:270	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	23	theme	officinale	379:388	arg1	ginger					362:367	ginger	362:367	ginger (Zingiber officinale Roscoe) stems	362:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	1	23	theme	officinale	379:388	arg1	Roscoe					390:395	Zingiber officinale Roscoe	370:395	Zingiber officinale Roscoe	370:395	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	4	24	theme	Scanning	732:739	arg1	microscopy					750:759	Scanning electron microscopy	732:759	Scanning electron microscopy	732:759	Scanning electron microscopy indicated that GSLP microstructures were greatly influenced by extraction method.					
32088222	3	25	theme	extraction	673:682	arg1	yield					684:688	highest extraction yield	665:688	highest extraction yield	665:688	The data showed that ASE produced highest extraction yield compared to the other extraction methods.					
32088222	2	26	theme	hot	491:493	arg1	extraction					501:510	hot water extraction	491:510	hot water extraction (HWE)	491:516	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	2	26	theme	hot	491:493	arg1	HWE					513:515	HWE	513:515	HWE	513:515	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	5	27	theme	in	853:854	arg1	experiments					909:919	in vitro hypoglycemic activity and antioxidant activity experiments	853:919	in vitro hypoglycemic activity and antioxidant activity experiments	853:919	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	6	28	theme	different	1209:1217	arg1	mechanisms					1234:1243	different characteristic mechanisms	1209:1243	different characteristic mechanisms of degradation	1209:1258	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	3	29	theme	other	706:710	arg1	methods					723:729	the other extraction methods	702:729	the other extraction methods	702:729	The data showed that ASE produced highest extraction yield compared to the other extraction methods.					
32088222	8	30	from	stems	1590:1594	arg1	polysaccharides					1569:1583	polysaccharides	1569:1583	polysaccharides from stems	1569:1594	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	4	31	theme	extraction	824:833	arg1	method					835:840	extraction method	824:840	extraction method	824:840	Scanning electron microscopy indicated that GSLP microstructures were greatly influenced by extraction method.					
32088222	7	32	theme	abundant	1391:1398	arg1	content					1412:1418	its abundant uronic acid content	1387:1418	its abundant uronic acid content	1387:1418	In addition, ASE-GSLP displayed better biological activities probably due to its abundant uronic acid content, higher sulfate radical, and smaller molecular weight.					
32088222	7	33	theme	biological	1349:1358	arg1	activities					1360:1369	better biological activities	1342:1369	better biological activities	1342:1369	In addition, ASE-GSLP displayed better biological activities probably due to its abundant uronic acid content, higher sulfate radical, and smaller molecular weight.					
32088222	2	34	theme	extraction	428:437	arg1	methods					439:445	Four extraction methods	423:445	Four extraction methods	423:445	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	1	35	theme	structure	273:281	arg1	characteristics					283:297	structure characteristics	273:297	structure characteristics	273:297	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	7	36	theme	higher	1421:1426	arg1	radical					1436:1442	higher sulfate radical	1421:1442	higher sulfate radical	1421:1442	In addition, ASE-GSLP displayed better biological activities probably due to its abundant uronic acid content, higher sulfate radical, and smaller molecular weight.					
32088222	5	37	dep	in	853:854	arg1	vitro					856:860	vitro	856:860	vitro	856:860	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	5	38	theme	biological	943:952	arg1	capacities					954:963	the biological capacities	939:963	the biological capacities of ASE-GSLP	939:975	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	5	38	theme	biological	943:952	arg1	superior					982:989	superior	982:989	superior	982:989	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	6	39	with	imbued	1197:1202	arg1	mechanisms					1234:1243	different characteristic mechanisms	1209:1243	different characteristic mechanisms of degradation	1209:1258	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	0	40	theme	different	100:108	arg1	methods					110:116	different methods	100:116	different methods	100:116	A comparison of a polysaccharide extracted from ginger (Zingiber officinale) stems and leaves using different methods: preparation, structure characteristics, and biological activities.					
32088222	6	41	theme	complex	1141:1147	arg1	enzyme					1149:1154	complex enzyme	1141:1154	complex enzyme	1141:1154	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	5	42	theme	hypoglycemic	862:873	arg1	activity					875:882	hypoglycemic activity	862:882	hypoglycemic activity	862:882	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	1	43	from	yield	244:248	arg1	stems					398:402	ginger (Zingiber officinale Roscoe) stems	362:402	ginger (Zingiber officinale Roscoe) stems	362:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	5	44	theme	other	1017:1021	arg1	methods					1023:1029	the other methods	1013:1029	the other methods	1013:1029	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	8	45	contain	has	1510:1512	arg2	strategy					1546:1553	an effective strategy	1533:1553	an effective strategy for obtaining polysaccharides from stems	1533:1594	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	8	45	contain	has	1510:1512	arg1	ASE					1506:1508	ASE	1506:1508	ASE	1506:1508	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	8	45	contain	has	1510:1512	arg2	potential					1520:1528	great potential	1514:1528	great potential	1514:1528	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	0	46	theme	polysaccharide	18:31	arg1	comparison					2:11	A comparison	0:11	A comparison of a polysaccharide extracted from ginger (Zingiber officinale)	0:75	A comparison of a polysaccharide extracted from ginger (Zingiber officinale) stems and leaves using different methods: preparation, structure characteristics, and biological activities.					
32088222	1	47	theme	hypoglycemic	300:311	arg1	activity					329:336	hypoglycemic and antioxidant activity	300:336	hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems	300:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	6	48	dep	ginger	1097:1102	arg1	leaves					1114:1119	leaves	1114:1119	leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions	1114:1191	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	6	48	dep	ginger	1097:1102	arg1	stems					1104:1108	stems	1104:1108	stems	1104:1108	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	2	49	theme	water	495:499	arg1	extraction					501:510	hot water extraction	491:510	hot water extraction (HWE)	491:516	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	2	49	theme	water	495:499	arg1	HWE					513:515	HWE	513:515	HWE	513:515	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	0	50	theme	structure	132:140	arg1	characteristics					142:156	structure characteristics	132:156	structure characteristics	132:156	A comparison of a polysaccharide extracted from ginger (Zingiber officinale) stems and leaves using different methods: preparation, structure characteristics, and biological activities.					
32088222	3	51	theme	extraction	712:721	arg1	methods					723:729	the other extraction methods	702:729	the other extraction methods	702:729	The data showed that ASE produced highest extraction yield compared to the other extraction methods.					
32088222	6	52	theme	degradation	1248:1258	arg1	mechanisms					1234:1243	different characteristic mechanisms	1209:1243	different characteristic mechanisms of degradation	1209:1258	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	3	53	theme	highest	665:671	arg1	yield					684:688	highest extraction yield	665:688	highest extraction yield	665:688	The data showed that ASE produced highest extraction yield compared to the other extraction methods.					
32088222	5	54	theme	activity	900:907	arg1	experiments					909:919	in vitro hypoglycemic activity and antioxidant activity experiments	853:919	in vitro hypoglycemic activity and antioxidant activity experiments	853:919	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	8	55	theme	great	1514:1518	arg1	potential					1520:1528	great potential	1514:1528	great potential	1514:1528	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	8	55	theme	great	1514:1518	arg1	strategy					1546:1553	an effective strategy	1533:1553	an effective strategy for obtaining polysaccharides from stems	1533:1594	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	1	56	theme	antioxidant	317:327	arg1	activity					329:336	hypoglycemic and antioxidant activity	300:336	hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems	300:402	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	7	57	theme	acid	1407:1410	arg1	content					1412:1418	its abundant uronic acid content	1387:1418	its abundant uronic acid content	1387:1418	In addition, ASE-GSLP displayed better biological activities probably due to its abundant uronic acid content, higher sulfate radical, and smaller molecular weight.					
32088222	6	58	theme	structures	1298:1307	arg1	uniformity					1273:1282	the uniformity	1269:1282	the uniformity of their main structures	1269:1307	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	7	59	theme	molecular	1457:1465	arg1	weight					1467:1472	smaller molecular weight	1449:1472	smaller molecular weight	1449:1472	In addition, ASE-GSLP displayed better biological activities probably due to its abundant uronic acid content, higher sulfate radical, and smaller molecular weight.					
32088222	1	60	theme	extraction	214:223	arg1	method					225:230	extraction method	214:230	extraction method	214:230	This study investigates how extraction method impacts the yield, chemical composition, structure characteristics, hypoglycemic and antioxidant activity of polysaccharides from ginger (Zingiber officinale Roscoe) stems and leaves (GSLP).					
32088222	6	61	theme	ultrasonic-assisted	1161:1179	arg1	extractions					1181:1191	ultrasonic-assisted extractions	1161:1191	ultrasonic-assisted extractions	1161:1191	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	6	62	theme	characteristic	1219:1232	arg1	mechanisms					1234:1243	different characteristic mechanisms	1209:1243	different characteristic mechanisms of degradation	1209:1258	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	0	63	theme	Zingiber	56:63	arg1	officinale					65:74	Zingiber officinale	56:74	Zingiber officinale	56:74	A comparison of a polysaccharide extracted from ginger (Zingiber officinale) stems and leaves using different methods: preparation, structure characteristics, and biological activities.					
32088222	0	63	theme	Zingiber	56:63	arg1	ginger					48:53	ginger	48:53	ginger (Zingiber officinale)	48:75	A comparison of a polysaccharide extracted from ginger (Zingiber officinale) stems and leaves using different methods: preparation, structure characteristics, and biological activities.					
32088222	4	64	theme	GSLP	776:779	arg1	microstructures					781:795	GSLP microstructures	776:795	GSLP microstructures	776:795	Scanning electron microscopy indicated that GSLP microstructures were greatly influenced by extraction method.					
32088222	6	65	from	ginger	1097:1102	arg1	polysaccharides					1076:1090	polysaccharides	1076:1090	polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions	1076:1191	Taken together, these results indicate that polysaccharides from ginger stems and leaves obtained by alkali, complex enzyme, and ultrasonic-assisted extractions are imbued with different characteristic mechanisms of degradation, despite the uniformity of their main structures.					
32088222	2	66	theme	ultrasound-assisted	519:537	arg1	UAE					551:553	UAE	551:553	UAE	551:553	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	2	66	theme	ultrasound-assisted	519:537	arg1	extraction					539:548	ultrasound-assisted extraction	519:548	ultrasound-assisted extraction (UAE)	519:554	Four extraction methods were employed to obtain the GSLP, including hot water extraction (HWE), ultrasound-assisted extraction (UAE), alkaline solution extraction (ASE), and enzyme-assisted extraction (EAE).					
32088222	8	67	theme	products	1673:1680	arg1	yield					1629:1633	its higher yield	1618:1633	its higher yield	1618:1633	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	8	67	theme	products	1673:1680	arg1	bioactivity					1654:1664	the remarkable bioactivity	1639:1664	the remarkable bioactivity of its products	1639:1680	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
32088222	5	68	theme	activity	875:882	arg1	experiments					909:919	in vitro hypoglycemic activity and antioxidant activity experiments	853:919	in vitro hypoglycemic activity and antioxidant activity experiments	853:919	Moreover, in vitro hypoglycemic activity and antioxidant activity experiments demonstrated that the biological capacities of ASE-GSLP were superior to GSLPs extracted by the other methods.					
32088222	8	69	theme	higher	1622:1627	arg1	yield					1629:1633	its higher yield	1618:1633	its higher yield	1618:1633	Thus, it can be concluded that ASE has great potential as an effective strategy for obtaining polysaccharides from stems and leaves because of its higher yield and the remarkable bioactivity of its products.					
34806724	0	0	theme	sodium-induced	106:119	arg1	colitis					121:127	dextran sulfate sodium-induced colitis	90:127	dextran sulfate sodium-induced colitis	90:127	Polysaccharides from Sargassum fusiforme after UV/H2O2 degradation effectively ameliorate dextran sulfate sodium-induced colitis.					
34806724	0	1	theme	sulfate	98:104	arg1	colitis					121:127	dextran sulfate sodium-induced colitis	90:127	dextran sulfate sodium-induced colitis	90:127	Polysaccharides from Sargassum fusiforme after UV/H2O2 degradation effectively ameliorate dextran sulfate sodium-induced colitis.					
34806724	1	2	from	fusiforme	185:193	arg1	polysaccharides					154:168	degraded polysaccharides	145:168	degraded polysaccharides from Sargassum fusiforme (PSF-T2)	145:202	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
34806724	4	3	theme	Lachnospiraceae	831:845	arg1	abundance					800:808	the abundance	796:808	the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae	796:887	Besides, PSF-T2 promoted the production of short-chain fatty acids and modulated gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae).					
34806724	4	4	theme	gut	757:759	arg1	composition					772:782	gut microbiota composition	757:782	gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae)	757:943	Besides, PSF-T2 promoted the production of short-chain fatty acids and modulated gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae).					
34806724	4	5	dep	composition	772:782	arg1	decreasing					894:903	decreasing	894:903	decreasing Bacteroidaceae and Erysipelotrichaceae	894:942	Besides, PSF-T2 promoted the production of short-chain fatty acids and modulated gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae).					
34806724	4	5	dep	composition	772:782	arg1	increasing					785:794	increasing	785:794	increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae	785:887	Besides, PSF-T2 promoted the production of short-chain fatty acids and modulated gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae).					
34806724	6	6	theme	polysaccharides	1228:1242	arg1	preparation					1203:1213	the preparation	1199:1213	the preparation of bioactive polysaccharides by free radical degradation	1199:1270	It can provide a theoretical basis for the preparation of bioactive polysaccharides by free radical degradation.					
34806724	5	7	theme	UV/H2O2	1022:1028	arg1	degradation					1030:1040	UV/H2O2 degradation	1022:1040	UV/H2O2 degradation	1022:1040	These results suggested that polysaccharides from Sargassum fusiforme after UV/H2O2 degradation could ameliorate colitis by decreasing inflammation, protecting the intestinal barrier and modulating gut microbiota.					
34806724	1	8	theme	ameliorating	267:278	arg1	sulfate					288:294	ameliorating dextran sulfate	267:294	ameliorating dextran sulfate sodium-induced colitis	267:317	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
34806724	4	9	theme	Oscillospiraceae	848:863	arg1	abundance					800:808	the abundance	796:808	the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae	796:887	Besides, PSF-T2 promoted the production of short-chain fatty acids and modulated gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae).					
34806724	6	10	theme	radical	1252:1258	arg1	degradation					1260:1270	free radical degradation	1247:1270	free radical degradation	1247:1270	It can provide a theoretical basis for the preparation of bioactive polysaccharides by free radical degradation.					
34806724	2	11	theme	activity	490:497	arg1	index					499:503	disease activity index	482:503	disease activity index	482:503	Results showed that PSF-T2 relieved colitis symptoms, characterized by increasing the colon length and body weight, decreasing disease activity index and relieving colon damage.					
34806724	4	12	theme	microbiota	761:770	arg1	composition					772:782	gut microbiota composition	757:782	gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae)	757:943	Besides, PSF-T2 promoted the production of short-chain fatty acids and modulated gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae).					
34806724	4	13	theme	Desulfovibrionaceae	869:887	arg1	abundance					800:808	the abundance	796:808	the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae	796:887	Besides, PSF-T2 promoted the production of short-chain fatty acids and modulated gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae).					
34806724	2	14	theme	colon	519:523	arg1	damage					525:530	colon damage	519:530	colon damage	519:530	Results showed that PSF-T2 relieved colitis symptoms, characterized by increasing the colon length and body weight, decreasing disease activity index and relieving colon damage.					
34806724	4	15	theme	fatty	731:735	arg1	acids					737:741	short-chain fatty acids	719:741	short-chain fatty acids	719:741	Besides, PSF-T2 promoted the production of short-chain fatty acids and modulated gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae).					
34806724	2	16	theme	body	458:461	arg1	weight					463:468	body weight	458:468	body weight	458:468	Results showed that PSF-T2 relieved colitis symptoms, characterized by increasing the colon length and body weight, decreasing disease activity index and relieving colon damage.					
34806724	1	17	theme	dextran	280:286	arg1	sulfate					288:294	ameliorating dextran sulfate	267:294	ameliorating dextran sulfate sodium-induced colitis	267:317	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
34806724	4	18	theme	short-chain	719:729	arg1	acids					737:741	short-chain fatty acids	719:741	short-chain fatty acids	719:741	Besides, PSF-T2 promoted the production of short-chain fatty acids and modulated gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae).					
34806724	2	19	theme	colitis	391:397	arg1	symptoms					399:406	colitis symptoms	391:406	colitis symptoms	391:406	Results showed that PSF-T2 relieved colitis symptoms, characterized by increasing the colon length and body weight, decreasing disease activity index and relieving colon damage.					
34806724	1	20	theme	sulfate	288:294	arg1	colitis					311:317	ameliorating dextran sulfate sodium-induced colitis	267:317	ameliorating dextran sulfate sodium-induced colitis	267:317	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
34806724	5	21	theme	Sargassum	996:1004	arg1	fusiforme					1006:1014	Sargassum fusiforme	996:1014	Sargassum fusiforme	996:1014	These results suggested that polysaccharides from Sargassum fusiforme after UV/H2O2 degradation could ameliorate colitis by decreasing inflammation, protecting the intestinal barrier and modulating gut microbiota.					
34806724	2	22	theme	colon	441:445	arg1	length					447:452	the colon length	437:452	the colon length	437:452	Results showed that PSF-T2 relieved colitis symptoms, characterized by increasing the colon length and body weight, decreasing disease activity index and relieving colon damage.					
34806724	0	23	theme	Sargassum	21:29	arg1	fusiforme					31:39	Sargassum fusiforme	21:39	Sargassum fusiforme	21:39	Polysaccharides from Sargassum fusiforme after UV/H2O2 degradation effectively ameliorate dextran sulfate sodium-induced colitis.					
34806724	1	24	theme	sodium-induced	296:309	arg1	colitis					311:317	ameliorating dextran sulfate sodium-induced colitis	267:317	ameliorating dextran sulfate sodium-induced colitis	267:317	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
34806724	5	25	theme	gut	1144:1146	arg1	microbiota					1148:1157	gut microbiota	1144:1157	gut microbiota	1144:1157	These results suggested that polysaccharides from Sargassum fusiforme after UV/H2O2 degradation could ameliorate colitis by decreasing inflammation, protecting the intestinal barrier and modulating gut microbiota.					
34806724	4	26	theme	acids	737:741	arg1	production					705:714	the production	701:714	the production of short-chain fatty acids	701:741	Besides, PSF-T2 promoted the production of short-chain fatty acids and modulated gut microbiota composition (increasing the abundance of Lactobacillaceae, Lachnospiraceae, Oscillospiraceae and Desulfovibrionaceae, and decreasing Bacteroidaceae and Erysipelotrichaceae).					
34806724	1	27	theme	UV/H2O2	221:227	arg1	treatment					229:237	UV/H2O2 treatment	221:237	UV/H2O2 treatment	221:237	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
34806724	0	28	theme	UV/H2O2	47:53	arg1	degradation					55:65	UV/H2O2 degradation	47:65	UV/H2O2 degradation	47:65	Polysaccharides from Sargassum fusiforme after UV/H2O2 degradation effectively ameliorate dextran sulfate sodium-induced colitis.					
34806724	3	29	theme	MUC-2	650:654	arg1	expression					636:645	the expression	632:645	the expression of MUC-2, ZO-1 and occludin	632:673	In addition, PSF-T2 decreased the secretion and expression of IL-1β, IL-6 and TNF-α, and increased the expression of MUC-2, ZO-1 and occludin.					
34806724	3	30	theme	IL-6	602:605	arg1	expression					581:590	expression	581:590	expression	581:590	In addition, PSF-T2 decreased the secretion and expression of IL-1β, IL-6 and TNF-α, and increased the expression of MUC-2, ZO-1 and occludin.					
34806724	3	30	theme	IL-6	602:605	arg1	secretion					567:575	secretion	567:575	secretion	567:575	In addition, PSF-T2 decreased the secretion and expression of IL-1β, IL-6 and TNF-α, and increased the expression of MUC-2, ZO-1 and occludin.					
34806724	2	31	theme	disease	482:488	arg1	index					499:503	disease activity index	482:503	disease activity index	482:503	Results showed that PSF-T2 relieved colitis symptoms, characterized by increasing the colon length and body weight, decreasing disease activity index and relieving colon damage.					
34806724	1	32	from	effects	256:262	arg1	colitis					311:317	ameliorating dextran sulfate sodium-induced colitis	267:317	ameliorating dextran sulfate sodium-induced colitis	267:317	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
34806724	3	33	theme	IL-1β	595:599	arg1	expression					581:590	expression	581:590	expression	581:590	In addition, PSF-T2 decreased the secretion and expression of IL-1β, IL-6 and TNF-α, and increased the expression of MUC-2, ZO-1 and occludin.					
34806724	3	33	theme	IL-1β	595:599	arg1	secretion					567:575	secretion	567:575	secretion	567:575	In addition, PSF-T2 decreased the secretion and expression of IL-1β, IL-6 and TNF-α, and increased the expression of MUC-2, ZO-1 and occludin.					
34806724	6	34	theme	theoretical	1177:1187	arg1	basis					1189:1193	a theoretical basis	1175:1193	a theoretical basis for the preparation of bioactive polysaccharides by free radical degradation	1175:1270	It can provide a theoretical basis for the preparation of bioactive polysaccharides by free radical degradation.					
34806724	1	35	theme	mouse	342:346	arg1	model					348:352	a mouse model	340:352	a mouse model	340:352	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
34806724	5	36	theme	intestinal	1110:1119	arg1	barrier					1121:1127	the intestinal barrier	1106:1127	the intestinal barrier	1106:1127	These results suggested that polysaccharides from Sargassum fusiforme after UV/H2O2 degradation could ameliorate colitis by decreasing inflammation, protecting the intestinal barrier and modulating gut microbiota.					
34806724	6	37	theme	bioactive	1218:1226	arg1	polysaccharides					1228:1242	bioactive polysaccharides	1218:1242	bioactive polysaccharides	1218:1242	It can provide a theoretical basis for the preparation of bioactive polysaccharides by free radical degradation.					
34806724	1	38	theme	degraded	145:152	arg1	polysaccharides					154:168	degraded polysaccharides	145:168	degraded polysaccharides from Sargassum fusiforme (PSF-T2)	145:202	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
34806724	5	39	from	fusiforme	1006:1014	arg1	polysaccharides					975:989	polysaccharides	975:989	polysaccharides from Sargassum fusiforme after UV/H2O2 degradation	975:1040	These results suggested that polysaccharides from Sargassum fusiforme after UV/H2O2 degradation could ameliorate colitis by decreasing inflammation, protecting the intestinal barrier and modulating gut microbiota.					
34806724	6	40	theme	free	1247:1250	arg1	degradation					1260:1270	free radical degradation	1247:1270	free radical degradation	1247:1270	It can provide a theoretical basis for the preparation of bioactive polysaccharides by free radical degradation.					
34806724	0	41	theme	dextran	90:96	arg1	sulfate					98:104	dextran sulfate	90:104	dextran sulfate sodium-induced colitis	90:127	Polysaccharides from Sargassum fusiforme after UV/H2O2 degradation effectively ameliorate dextran sulfate sodium-induced colitis.					
34806724	3	42	theme	TNF-α	611:615	arg1	expression					581:590	expression	581:590	expression	581:590	In addition, PSF-T2 decreased the secretion and expression of IL-1β, IL-6 and TNF-α, and increased the expression of MUC-2, ZO-1 and occludin.					
34806724	3	42	theme	TNF-α	611:615	arg1	secretion					567:575	secretion	567:575	secretion	567:575	In addition, PSF-T2 decreased the secretion and expression of IL-1β, IL-6 and TNF-α, and increased the expression of MUC-2, ZO-1 and occludin.					
34806724	3	43	dep	secretion	567:575	arg1	the					563:565	the	563:565	the	563:565	In addition, PSF-T2 decreased the secretion and expression of IL-1β, IL-6 and TNF-α, and increased the expression of MUC-2, ZO-1 and occludin.					
34806724	0	44	from	fusiforme	31:39	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Sargassum fusiforme after UV/H2O2 degradation	0:65	Polysaccharides from Sargassum fusiforme after UV/H2O2 degradation effectively ameliorate dextran sulfate sodium-induced colitis.					
34806724	1	45	theme	Sargassum	175:183	arg1	PSF-T2					196:201	PSF-T2	196:201	PSF-T2	196:201	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
34806724	1	45	theme	Sargassum	175:183	arg1	fusiforme					185:193	Sargassum fusiforme	175:193	Sargassum fusiforme (PSF-T2)	175:202	In this study, degraded polysaccharides from Sargassum fusiforme (PSF-T2) were prepared by UV/H2O2 treatment for 2 h, and its effects on ameliorating dextran sulfate sodium-induced colitis were evaluated using a mouse model.					
33463881	12	0	theme	protein	1267:1273	arg1	content					1275:1281	protein content	1267:1281	protein content	1267:1281	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	6	1	theme	year	665:668	arg1	harvest					633:639	the harvest	629:639	the harvest of the warmer and drier year (2018)	629:675	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	1	2	theme	petrochemical	137:149	arg1	industry					151:158	the petrochemical industry	133:158	the petrochemical industry towards more sustainable and bio-based production processes for fuels and speciality chemicals	133:253	The current threats of climate change are driving attention away from the petrochemical industry towards more sustainable and bio-based production processes for fuels and speciality chemicals.					
33463881	11	3	theme	drier	1170:1174	arg1	harvest					1181:1187	the warmer and drier 2018 harvest	1155:1187	the warmer and drier 2018 harvest	1155:1187	A lignocellulose reduction of 25% was recorded in the warmer and drier 2018 harvest.					
33463881	10	4	theme	weather	1057:1063	arg1	variations					1065:1074	weather variations	1057:1074	weather variations	1057:1074	However, this composition is greatly influenced by weather variations during the oat growth phase.					
33463881	11	5	theme	2018	1176:1179	arg1	harvest					1181:1187	the warmer and drier 2018 harvest	1155:1187	the warmer and drier 2018 harvest	1155:1187	A lignocellulose reduction of 25% was recorded in the warmer and drier 2018 harvest.					
33463881	11	6	theme	%	1137:1137	arg1	reduction					1122:1130	A lignocellulose reduction	1105:1130	A lignocellulose reduction of 25%	1105:1137	A lignocellulose reduction of 25% was recorded in the warmer and drier 2018 harvest.					
33463881	7	7	used	utilized	788:795	arg2	methods					687:693	Standard methods	678:693	Standard methods	678:693	Standard methods for determination of plant chemical composition, with focus on carbohydrate composition, are utilized.					
33463881	8	8	theme	Oat	798:800	arg1	hulls					802:806	Oat hulls	798:806	Oat hulls grown in 'normal' weather conditions (2017)	798:850	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	14	9	theme	drier	1588:1592	arg1	weather					1594:1600	warmer and drier weather	1577:1600	warmer and drier weather	1577:1600	However, this characteristic is significantly reduced when grown in warmer and drier weather, which could compromise its suitability for use in a successful biorefinery.					
33463881	6	10	dep	hulls	524:528	arg1	Kerstin					531:537	Kerstin	531:537	Kerstin	531:537	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	6	10	dep	hulls	524:528	arg1	hulls					524:528	Oat hulls	520:528	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017)	520:611	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	6	10	dep	hulls	524:528	arg1	Galant					543:548	Galant	543:548	Galant	543:548	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	12	11	from	increase	1255:1262	arg1	content					1314:1320	phenolic content	1305:1320	phenolic content	1305:1320	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	12	11	from	increase	1255:1262	arg1	content					1234:1240	starch content	1227:1240	starch content	1227:1240	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	12	11	from	increase	1255:1262	arg1	content					1275:1281	protein content	1267:1281	protein content	1267:1281	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	12	12	theme	starch	1227:1232	arg1	content					1234:1240	starch content	1227:1240	starch content	1227:1240	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	10	13	theme	growth	1091:1096	arg1	phase					1098:1102	the oat growth phase	1083:1102	the oat growth phase	1083:1102	However, this composition is greatly influenced by weather variations during the oat growth phase.					
33463881	6	14	theme	Oat	520:522	arg1	Kerstin					531:537	Kerstin	531:537	Kerstin	531:537	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	6	14	theme	Oat	520:522	arg1	hulls					524:528	Oat hulls	520:528	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017)	520:611	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	6	14	theme	Oat	520:522	arg1	Galant					543:548	Galant	543:548	Galant	543:548	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	12	15	theme	four-fold	1245:1253	arg1	increase					1255:1262	a four-fold increase	1243:1262	a four-fold increase in protein content	1243:1281	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	8	16	theme	25	919:920	arg1	%					921:921	%	921:921	%	921:921	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	2	17	theme	starting	298:305	arg1	material					307:314	suitable low-cost starting material	280:314	suitable low-cost starting material	280:314	These processes require suitable low-cost starting material.					
33463881	6	18	theme	drier	659:663	arg1	2018					671:674	2018	671:674	2018	671:674	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	6	18	theme	drier	659:663	arg1	year					665:668	the warmer and drier year	644:668	the warmer and drier year (2018)	644:675	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	3	19	theme	oat	361:363	arg1	hull					365:368	the oat hull	357:368	the oat hull	357:368	One potential material assessed here is the oat hull.					
33463881	3	19	theme	oat	361:363	arg1	material					331:338	One potential material	317:338	One potential material assessed here	317:352	One potential material assessed here is the oat hull.					
33463881	6	20	dep	Kerstin	531:537	arg1	varieties					550:558	varieties	550:558	varieties	550:558	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	8	21	from	lignocellulose	864:877	arg1	rich					856:859	rich	856:859	rich	856:859	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	11	22	theme	lignocellulose	1107:1120	arg1	reduction					1122:1130	A lignocellulose reduction	1105:1130	A lignocellulose reduction of 25%	1105:1137	A lignocellulose reduction of 25% was recorded in the warmer and drier 2018 harvest.					
33463881	4	23	theme	chemical	383:390	arg1	composition					392:402	Its overall chemical composition	371:402	Its overall chemical composition	371:402	Its overall chemical composition has so far not been fully characterized.					
33463881	1	24	theme	current	67:73	arg1	threats					75:81	The current threats	63:81	The current threats of climate change	63:99	The current threats of climate change are driving attention away from the petrochemical industry towards more sustainable and bio-based production processes for fuels and speciality chemicals.					
33463881	5	25	theme	extreme	496:502	arg1	events					512:517	extreme weather events	496:517	extreme weather events	496:517	Furthermore, it is not known how it is affected by extreme weather events.					
33463881	6	26	theme	weather	583:589	arg1	years					591:595	'normal' weather years	574:595	'normal' weather years (2016 and 2017)	574:611	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	8	27	theme	%	936:936	arg1	cellulose					938:946	23% cellulose	934:946	23% cellulose	934:946	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	4	28	theme	overall	375:381	arg1	composition					392:402	Its overall chemical composition	371:402	Its overall chemical composition	371:402	Its overall chemical composition has so far not been fully characterized.					
33463881	8	29	theme	weather	826:832	arg1	2017					846:849	2017	846:849	2017	846:849	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	8	29	theme	weather	826:832	arg1	conditions					834:843	'normal' weather conditions	817:843	'normal' weather conditions (2017)	817:850	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	5	30	theme	weather	504:510	arg1	events					512:517	extreme weather events	496:517	extreme weather events	496:517	Furthermore, it is not known how it is affected by extreme weather events.					
33463881	1	31	theme	sustainable	173:183	arg1	processes					210:218	more sustainable and bio-based production processes	168:218	more sustainable and bio-based production processes for fuels and speciality chemicals	168:253	The current threats of climate change are driving attention away from the petrochemical industry towards more sustainable and bio-based production processes for fuels and speciality chemicals.					
33463881	0	32	theme	Warming	0:6	arg1	weather					8:14	Warming weather	0:14	Warming weather	0:14	Warming weather changes the chemical composition of oat hulls.					
33463881	13	33	theme	large	1399:1403	arg1	fraction					1419:1426	an exceptionally large hemicellulose fraction	1382:1426	an exceptionally large hemicellulose fraction	1382:1426	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	7	34	from	focus	749:753	arg1	composition					771:781	carbohydrate composition	758:781	carbohydrate composition	758:781	Standard methods for determination of plant chemical composition, with focus on carbohydrate composition, are utilized.					
33463881	8	35	theme	%	902:902	arg1	hemicellulose					904:916	35% hemicellulose	900:916	35% hemicellulose	900:916	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	13	36	theme	agricultural	1483:1494	arg1	by-products					1496:1506	agricultural by-products	1483:1506	agricultural by-products	1483:1506	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	12	37	from	increase	1215:1222	arg1	content					1314:1320	phenolic content	1305:1320	phenolic content	1305:1320	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	12	37	from	increase	1215:1222	arg1	content					1234:1240	starch content	1227:1240	starch content	1227:1240	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	12	37	from	increase	1215:1222	arg1	content					1275:1281	protein content	1267:1281	protein content	1267:1281	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	6	38	theme	normal	575:580	arg1	years					591:595	'normal' weather years	574:595	'normal' weather years (2016 and 2017)	574:611	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	13	39	theme	hulls	1461:1465	arg1	unique					1470:1475	unique	1470:1475	unique	1470:1475	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	13	39	theme	hulls	1461:1465	arg1	composition					1442:1452	the chemical composition	1429:1452	the chemical composition of oat hulls	1429:1465	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	8	40	theme	35	900:901	arg1	%					902:902	%	902:902	%	902:902	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	1	41	theme	climate	86:92	arg1	change					94:99	climate change	86:99	climate change	86:99	The current threats of climate change are driving attention away from the petrochemical industry towards more sustainable and bio-based production processes for fuels and speciality chemicals.					
33463881	8	42	theme	normal	818:823	arg1	2017					846:849	2017	846:849	2017	846:849	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	8	42	theme	normal	818:823	arg1	conditions					834:843	'normal' weather conditions	817:843	'normal' weather conditions (2017)	817:850	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	1	43	theme	bio-based	189:197	arg1	processes					210:218	more sustainable and bio-based production processes	168:218	more sustainable and bio-based production processes for fuels and speciality chemicals	168:253	The current threats of climate change are driving attention away from the petrochemical industry towards more sustainable and bio-based production processes for fuels and speciality chemicals.					
33463881	11	44	theme	warmer	1159:1164	arg1	harvest					1181:1187	the warmer and drier 2018 harvest	1155:1187	the warmer and drier 2018 harvest	1155:1187	A lignocellulose reduction of 25% was recorded in the warmer and drier 2018 harvest.					
33463881	7	45	theme	composition	731:741	arg1	determination					699:711	determination	699:711	determination of plant chemical composition	699:741	Standard methods for determination of plant chemical composition, with focus on carbohydrate composition, are utilized.					
33463881	1	46	theme	change	94:99	arg1	threats					75:81	The current threats	63:81	The current threats of climate change	63:99	The current threats of climate change are driving attention away from the petrochemical industry towards more sustainable and bio-based production processes for fuels and speciality chemicals.					
33463881	1	47	theme	production	199:208	arg1	processes					210:218	more sustainable and bio-based production processes	168:218	more sustainable and bio-based production processes for fuels and speciality chemicals	168:253	The current threats of climate change are driving attention away from the petrochemical industry towards more sustainable and bio-based production processes for fuels and speciality chemicals.					
33463881	12	48	theme	phenolic	1305:1312	arg1	content					1314:1320	phenolic content	1305:1320	phenolic content	1305:1320	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	13	49	theme	high	1344:1347	arg1	composition					1364:1374	its high lignocellulose composition	1340:1374	its high lignocellulose composition	1340:1374	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	7	50	theme	carbohydrate	758:769	arg1	composition					771:781	carbohydrate composition	758:781	carbohydrate composition	758:781	Standard methods for determination of plant chemical composition, with focus on carbohydrate composition, are utilized.					
33463881	0	51	theme	chemical	28:35	arg1	composition					37:47	the chemical composition	24:47	the chemical composition of oat hulls	24:60	Warming weather changes the chemical composition of oat hulls.					
33463881	7	52	theme	plant	716:720	arg1	composition					731:741	plant chemical composition	716:741	plant chemical composition	716:741	Standard methods for determination of plant chemical composition, with focus on carbohydrate composition, are utilized.					
33463881	12	53	from	decrease	1293:1300	arg1	content					1314:1320	phenolic content	1305:1320	phenolic content	1305:1320	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	12	53	from	decrease	1293:1300	arg1	content					1234:1240	starch content	1227:1240	starch content	1227:1240	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	12	53	from	decrease	1293:1300	arg1	content					1275:1281	protein content	1267:1281	protein content	1267:1281	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	13	54	theme	lignocellulose	1349:1362	arg1	composition					1364:1374	its high lignocellulose composition	1340:1374	its high lignocellulose composition	1340:1374	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	9	55	theme	major	982:986	arg1	Arabinoxylan					949:960	Arabinoxylan	949:960	Arabinoxylan	949:960	Arabinoxylan was found to be the major biopolymer (32%).					
33463881	9	55	theme	major	982:986	arg1	biopolymer					988:997	the major biopolymer	978:997	the major biopolymer (32%)	978:1003	Arabinoxylan was found to be the major biopolymer (32%).					
33463881	9	55	theme	major	982:986	arg1	%					1002:1002	32%	1000:1002	32%	1000:1002	Arabinoxylan was found to be the major biopolymer (32%).					
33463881	8	56	from	rich	856:859	arg1	lignocellulose					864:877	lignocellulose	864:877	lignocellulose (84%)	864:883	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	8	56	from	rich	856:859	arg1	%					882:882	84%	880:882	84%	880:882	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	0	57	theme	oat	52:54	arg1	hulls					56:60	oat hulls	52:60	oat hulls	52:60	Warming weather changes the chemical composition of oat hulls.					
33463881	7	58	theme	chemical	722:729	arg1	composition					731:741	plant chemical composition	716:741	plant chemical composition	716:741	Standard methods for determination of plant chemical composition, with focus on carbohydrate composition, are utilized.					
33463881	3	59	theme	potential	321:329	arg1	hull					365:368	the oat hull	357:368	the oat hull	357:368	One potential material assessed here is the oat hull.					
33463881	3	59	theme	potential	321:329	arg1	material					331:338	One potential material	317:338	One potential material assessed here	317:352	One potential material assessed here is the oat hull.					
33463881	14	60	theme	warmer	1577:1582	arg1	weather					1594:1600	warmer and drier weather	1577:1600	warmer and drier weather	1577:1600	However, this characteristic is significantly reduced when grown in warmer and drier weather, which could compromise its suitability for use in a successful biorefinery.					
33463881	6	61	dep	years	591:595	arg1	2016					598:601	2016	598:601	2016	598:601	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	6	61	dep	years	591:595	arg1	2017					607:610	2017	607:610	2017	607:610	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	12	62	theme	%	1291:1291	arg1	decrease					1293:1300	a 60% decrease	1287:1300	a 60% decrease in phenolic content	1287:1320	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	13	63	theme	oat	1457:1459	arg1	hulls					1461:1465	oat hulls	1457:1465	oat hulls	1457:1465	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	8	64	theme	%	921:921	arg1	lignin					923:928	25% lignin	919:928	25% lignin	919:928	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	10	65	theme	oat	1087:1089	arg1	phase					1098:1102	the oat growth phase	1083:1102	the oat growth phase	1083:1102	However, this composition is greatly influenced by weather variations during the oat growth phase.					
33463881	12	66	theme	60	1289:1290	arg1	%					1291:1291	%	1291:1291	%	1291:1291	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	12	67	theme	6.6-fold	1206:1213	arg1	increase					1215:1222	a 6.6-fold increase	1204:1222	a 6.6-fold increase in starch content	1204:1240	Additionally, a 6.6-fold increase in starch content, a four-fold increase in protein content and a 60% decrease in phenolic content was noted.					
33463881	2	68	theme	suitable	280:287	arg1	material					307:314	suitable low-cost starting material	280:314	suitable low-cost starting material	280:314	These processes require suitable low-cost starting material.					
33463881	13	69	theme	chemical	1433:1440	arg1	unique					1470:1475	unique	1470:1475	unique	1470:1475	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	13	69	theme	chemical	1433:1440	arg1	composition					1442:1452	the chemical composition	1429:1452	the chemical composition of oat hulls	1429:1465	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	13	70	theme	hemicellulose	1405:1417	arg1	fraction					1419:1426	an exceptionally large hemicellulose fraction	1382:1426	an exceptionally large hemicellulose fraction	1382:1426	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	6	71	theme	warmer	648:653	arg1	2018					671:674	2018	671:674	2018	671:674	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	6	71	theme	warmer	648:653	arg1	year					665:668	the warmer and drier year	644:668	the warmer and drier year (2018)	644:675	Oat hulls (Kerstin and Galant varieties) grown during 'normal' weather years (2016 and 2017) are compared to the harvest of the warmer and drier year (2018).					
33463881	8	72	theme	23	934:935	arg1	%					936:936	%	936:936	%	936:936	Oat hulls grown in 'normal' weather conditions (2017) are rich in lignocellulose (84%), consisting of 35% hemicellulose, 25% lignin and 23% cellulose.					
33463881	13	73	with	unique	1470:1475	arg1	fraction					1419:1426	an exceptionally large hemicellulose fraction	1382:1426	an exceptionally large hemicellulose fraction	1382:1426	Due to its high lignocellulose composition, with an exceptionally large hemicellulose fraction, the chemical composition of oat hulls is unique among agricultural by-products.					
33463881	14	74	theme	successful	1655:1664	arg1	biorefinery					1666:1676	a successful biorefinery	1653:1676	a successful biorefinery	1653:1676	However, this characteristic is significantly reduced when grown in warmer and drier weather, which could compromise its suitability for use in a successful biorefinery.					
33463881	1	75	theme	speciality	234:243	arg1	chemicals					245:253	speciality chemicals	234:253	speciality chemicals	234:253	The current threats of climate change are driving attention away from the petrochemical industry towards more sustainable and bio-based production processes for fuels and speciality chemicals.					
33463881	0	76	theme	hulls	56:60	arg1	composition					37:47	the chemical composition	24:47	the chemical composition of oat hulls	24:60	Warming weather changes the chemical composition of oat hulls.					
33463881	7	77	with	methods	687:693	arg1	focus					749:753	focus	749:753	focus on carbohydrate composition	749:781	Standard methods for determination of plant chemical composition, with focus on carbohydrate composition, are utilized.					
33463881	2	78	dep	suitable	280:287	arg1	low-cost					289:296	low-cost	289:296	low-cost	289:296	These processes require suitable low-cost starting material.					
33463881	7	79	theme	Standard	678:685	arg1	methods					687:693	Standard methods	678:693	Standard methods	678:693	Standard methods for determination of plant chemical composition, with focus on carbohydrate composition, are utilized.					
32651947	6	0	theme	monomer	992:998	arg1	composition					1000:1010	monomer composition	992:1010	monomer composition	992:1010	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	5	1	theme	fucoidans	899:907	arg1	range					890:894	a broad range	882:894	a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales	882:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	0	2	theme	brown	90:94	arg1	algae					96:100	brown algae	90:100	brown algae	90:100	Ion-exchange purification and structural characterization of five sulfated fucoidans from brown algae.					
32651947	7	3	theme	subsequent	1420:1429	arg1	studies					1441:1447	subsequent molecular studies	1420:1447	subsequent molecular studies	1420:1447	This study emphasizes the use of standardized ion-exchange chromatography to obtain defined fucoidans for subsequent molecular studies.					
32651947	5	4	from	orders	932:937	arg1	fucoidans					899:907	fucoidans	899:907	fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales	899:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	4	from	orders	932:937	arg1	range					890:894	a broad range	882:894	a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales	882:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	4	5	theme	ion-exchange	611:622	arg1	chromatography					624:637	ion-exchange chromatography	611:637	ion-exchange chromatography	611:637	Here, we use ion-exchange chromatography to purify different fucoidans and found a high structural diversity between fucoidans.					
32651947	7	6	theme	chromatography	1373:1386	arg1	use					1340:1342	the use	1336:1342	the use of standardized ion-exchange chromatography to obtain defined fucoidans for subsequent molecular studies	1336:1447	This study emphasizes the use of standardized ion-exchange chromatography to obtain defined fucoidans for subsequent molecular studies.					
32651947	5	7	from	range	890:894	arg1	orders					932:937	major brown algal orders	914:937	major brown algal orders including Ectocarpales, Laminariales and Fucales	914:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	7	from	range	890:894	arg1	Fucales					980:986	Fucales	980:986	Fucales	980:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	7	from	range	890:894	arg1	Laminariales					963:974	Laminariales	963:974	Laminariales	963:974	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	3	8	theme	standardized	561:572	arg1	protocols					587:595	standardized purification protocols	561:595	standardized purification protocols	561:595	Additionally, fucoidans are structurally diverse depending on species and season, urging the need for standardized purification protocols.					
32651947	2	9	theme	Standardized	269:280	arg1	work					282:285	Standardized work	269:285	Standardized work with fucoidans	269:300	Standardized work with fucoidans is required for structure-function studies, but remains challenging since available fucoidan preparations are often contaminated with other algal compounds.					
32651947	1	10	theme	potential	252:260	arg1	they					243:246	they	243:246	they	243:246	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	1	10	theme	potential	252:260	arg1	drugs					262:266	potential drugs	252:266	potential drugs	252:266	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	6	11	theme	Durvillaea	1219:1228	arg1	potatorum					1230:1238	Durvillaea potatorum	1219:1238	Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches	1219:1311	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	7	12	theme	molecular	1431:1439	arg1	studies					1441:1447	subsequent molecular studies	1420:1447	subsequent molecular studies	1420:1447	This study emphasizes the use of standardized ion-exchange chromatography to obtain defined fucoidans for subsequent molecular studies.					
32651947	6	13	theme	certain	1153:1159	arg1	fucoidans					1161:1169	certain fucoidans	1153:1169	certain fucoidans	1153:1169	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	1	14	theme	cell	177:180	arg1	wall					182:185	the cell wall	173:185	the cell wall of brown algae	173:200	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	6	15	link	α-1,3-linked	1245:1256	arg1	backbone					1265:1272	α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches	1245:1311	backbone	1265:1272	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	5	16	theme	broad	884:888	arg1	range					890:894	a broad range	882:894	a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales	882:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	4	17	theme	high	681:684	arg1	diversity					697:705	a high structural diversity	679:705	a high structural diversity between fucoidans	679:723	Here, we use ion-exchange chromatography to purify different fucoidans and found a high structural diversity between fucoidans.					
32651947	6	18	theme	fucose	1258:1263	arg1	backbone					1265:1272	α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches	1245:1311	backbone	1265:1272	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	2	19	theme	fucoidan	386:393	arg1	preparations					395:406	available fucoidan preparations	376:406	available fucoidan preparations	376:406	Standardized work with fucoidans is required for structure-function studies, but remains challenging since available fucoidan preparations are often contaminated with other algal compounds.					
32651947	0	20	theme	Ion-exchange	0:11	arg1	purification					13:24	Ion-exchange purification	0:24	Ion-exchange purification	0:24	Ion-exchange purification and structural characterization of five sulfated fucoidans from brown algae.					
32651947	6	21	theme	glucuronic	1089:1098	arg1	acid					1083:1086	galacturonic acid	1070:1086	galacturonic acid	1070:1086	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	21	theme	glucuronic	1089:1098	arg1	acid					1100:1103	glucuronic acid	1089:1103	glucuronic acid	1089:1103	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	21	theme	glucuronic	1089:1098	arg1	features					1141:1148	new structural features	1126:1148	new structural features of certain fucoidans	1126:1169	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	21	theme	glucuronic	1089:1098	arg1	O-acetylation					1109:1121	O-acetylation	1109:1121	O-acetylation	1109:1121	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	5	22	theme	algal	926:930	arg1	orders					932:937	major brown algal orders	914:937	major brown algal orders including Ectocarpales, Laminariales and Fucales	914:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	22	theme	algal	926:930	arg1	Fucales					980:986	Fucales	980:986	Fucales	980:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	22	theme	algal	926:930	arg1	Laminariales					963:974	Laminariales	963:974	Laminariales	963:974	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	6	23	theme	α-1,3-linked	1245:1256	arg1	backbone					1265:1272	α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches	1245:1311	backbone	1265:1272	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	2	24	theme	available	376:384	arg1	preparations					395:406	available fucoidan preparations	376:406	available fucoidan preparations	376:406	Standardized work with fucoidans is required for structure-function studies, but remains challenging since available fucoidan preparations are often contaminated with other algal compounds.					
32651947	0	25	theme	structural	30:39	arg1	characterization					41:56	structural characterization	30:56	structural characterization	30:56	Ion-exchange purification and structural characterization of five sulfated fucoidans from brown algae.					
32651947	7	26	theme	standardized	1347:1358	arg1	chromatography					1373:1386	standardized ion-exchange chromatography	1347:1386	standardized ion-exchange chromatography	1347:1386	This study emphasizes the use of standardized ion-exchange chromatography to obtain defined fucoidans for subsequent molecular studies.					
32651947	3	27	theme	purification	574:585	arg1	protocols					587:595	standardized purification protocols	561:595	standardized purification protocols	561:595	Additionally, fucoidans are structurally diverse depending on species and season, urging the need for standardized purification protocols.					
32651947	2	28	theme	other	436:440	arg1	compounds					448:456	other algal compounds	436:456	other algal compounds	436:456	Standardized work with fucoidans is required for structure-function studies, but remains challenging since available fucoidan preparations are often contaminated with other algal compounds.					
32651947	2	29	theme	algal	442:446	arg1	compounds					448:456	other algal compounds	436:456	other algal compounds	436:456	Standardized work with fucoidans is required for structure-function studies, but remains challenging since available fucoidan preparations are often contaminated with other algal compounds.					
32651947	0	30	from	algae	96:100	arg1	purification					13:24	Ion-exchange purification	0:24	Ion-exchange purification	0:24	Ion-exchange purification and structural characterization of five sulfated fucoidans from brown algae.					
32651947	0	30	from	algae	96:100	arg1	characterization					41:56	structural characterization	30:56	structural characterization	30:56	Ion-exchange purification and structural characterization of five sulfated fucoidans from brown algae.					
32651947	6	31	theme	structural	1130:1139	arg1	acid					1083:1086	galacturonic acid	1070:1086	galacturonic acid	1070:1086	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	31	theme	structural	1130:1139	arg1	acid					1100:1103	glucuronic acid	1089:1103	glucuronic acid	1089:1103	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	31	theme	structural	1130:1139	arg1	features					1141:1148	new structural features	1126:1148	new structural features of certain fucoidans	1126:1169	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	31	theme	structural	1130:1139	arg1	O-acetylation					1109:1121	O-acetylation	1109:1121	O-acetylation	1109:1121	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	32	theme	NMR	1034:1036	arg1	characterization					1038:1053	NMR characterization	1034:1053	NMR characterization	1034:1053	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	4	33	theme	different	649:657	arg1	fucoidans					659:667	different fucoidans	649:667	different fucoidans	649:667	Here, we use ion-exchange chromatography to purify different fucoidans and found a high structural diversity between fucoidans.					
32651947	4	34	dep	use	607:609	arg1	found					673:677	found	673:677	found a high structural diversity between fucoidans	673:723	Here, we use ion-exchange chromatography to purify different fucoidans and found a high structural diversity between fucoidans.					
32651947	4	34	dep	use	607:609	arg1	purify					642:647	purify	642:647	to purify different fucoidans	639:667	Here, we use ion-exchange chromatography to purify different fucoidans and found a high structural diversity between fucoidans.					
32651947	1	35	theme	brown	190:194	arg1	algae					196:200	brown algae	190:200	brown algae	190:200	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	5	36	theme	major	914:918	arg1	orders					932:937	major brown algal orders	914:937	major brown algal orders including Ectocarpales, Laminariales and Fucales	914:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	36	theme	major	914:918	arg1	Fucales					980:986	Fucales	980:986	Fucales	980:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	36	theme	major	914:918	arg1	Laminariales					963:974	Laminariales	963:974	Laminariales	963:974	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	4	37	theme	structural	686:695	arg1	diversity					697:705	a high structural diversity	679:705	a high structural diversity between fucoidans	679:723	Here, we use ion-exchange chromatography to purify different fucoidans and found a high structural diversity between fucoidans.					
32651947	1	38	theme	various	220:226	arg1	bioactivities					228:240	their various bioactivities	214:240	their various bioactivities	214:240	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	5	39	theme	brown	920:924	arg1	orders					932:937	major brown algal orders	914:937	major brown algal orders including Ectocarpales, Laminariales and Fucales	914:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	39	theme	brown	920:924	arg1	Fucales					980:986	Fucales	980:986	Fucales	980:986	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	39	theme	brown	920:924	arg1	Laminariales					963:974	Laminariales	963:974	Laminariales	963:974	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	0	40	theme	sulfated	66:73	arg1	fucoidans					75:83	five sulfated fucoidans	61:83	five sulfated fucoidans	61:83	Ion-exchange purification and structural characterization of five sulfated fucoidans from brown algae.					
32651947	6	41	with	potatorum	1230:1238	arg1	branches					1304:1311	α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches	1245:1311	branches	1304:1311	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	41	with	potatorum	1230:1238	arg1	β-1,6					1278:1282	α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches	1245:1311	β-1,6	1278:1282	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	41	with	potatorum	1230:1238	arg1	backbone					1265:1272	α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches	1245:1311	backbone	1265:1272	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	5	42	dep	polysaccharides	778:792	arg1	laminarin					807:815	laminarin	807:815	laminarin	807:815	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	42	dep	polysaccharides	778:792	arg1	phlorotannins					861:873	phlorotannins	861:873	phlorotannins	861:873	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	42	dep	polysaccharides	778:792	arg1	alginate					794:801	alginate	794:801	alginate	794:801	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	42	dep	polysaccharides	778:792	arg1	proteins					848:855	proteins	848:855	proteins	848:855	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	42	dep	polysaccharides	778:792	arg1	polysaccharides					778:792	the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins	774:873	polysaccharides	778:792	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	1	43	theme	diverse	119:125	arg1	Fucoidans					103:111	Fucoidans	103:111	Fucoidans	103:111	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	1	43	theme	diverse	119:125	arg1	class					127:131	a diverse class	117:131	a diverse class of sulfated polysaccharides integral to the cell wall of brown algae	117:200	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	6	44	theme	linkage	1013:1019	arg1	analysis					1021:1028	linkage analysis	1013:1028	linkage analysis	1013:1028	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	7	45	theme	defined	1398:1404	arg1	fucoidans					1406:1414	defined fucoidans	1398:1414	defined fucoidans for subsequent molecular studies	1398:1447	This study emphasizes the use of standardized ion-exchange chromatography to obtain defined fucoidans for subsequent molecular studies.					
32651947	7	46	theme	ion-exchange	1360:1371	arg1	chromatography					1373:1386	standardized ion-exchange chromatography	1347:1386	standardized ion-exchange chromatography	1347:1386	This study emphasizes the use of standardized ion-exchange chromatography to obtain defined fucoidans for subsequent molecular studies.					
32651947	5	47	theme	Ion-exchange	726:737	arg1	chromatography					739:752	Ion-exchange chromatography	726:752	Ion-exchange chromatography	726:752	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	2	48	theme	structure-function	318:335	arg1	studies					337:343	structure-function studies	318:343	structure-function studies	318:343	Standardized work with fucoidans is required for structure-function studies, but remains challenging since available fucoidan preparations are often contaminated with other algal compounds.					
32651947	6	49	theme	galacturonic	1070:1081	arg1	acid					1083:1086	galacturonic acid	1070:1086	galacturonic acid	1070:1086	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	49	theme	galacturonic	1070:1081	arg1	acid					1100:1103	glucuronic acid	1089:1103	glucuronic acid	1089:1103	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	49	theme	galacturonic	1070:1081	arg1	features					1141:1148	new structural features	1126:1148	new structural features of certain fucoidans	1126:1169	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	49	theme	galacturonic	1070:1081	arg1	O-acetylation					1109:1121	O-acetylation	1109:1121	O-acetylation	1109:1121	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	1	50	theme	sulfated	136:143	arg1	polysaccharides					145:159	sulfated polysaccharides	136:159	sulfated polysaccharides	136:159	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	6	51	theme	fucoidan	1205:1212	arg1	structure					1192:1200	a novel structure	1184:1200	a novel structure of fucoidan	1184:1212	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	5	52	theme	other	821:825	arg1	phlorotannins					861:873	phlorotannins	861:873	phlorotannins	861:873	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	52	theme	other	821:825	arg1	contaminants					827:838	other contaminants	821:838	other contaminants such as proteins and phlorotannins	821:873	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	5	52	theme	other	821:825	arg1	proteins					848:855	proteins	848:855	proteins	848:855	Ion-exchange chromatography efficiently removes the polysaccharides alginate and laminarin and other contaminants such as proteins and phlorotannins across a broad range of fucoidans from major brown algal orders including Ectocarpales, Laminariales and Fucales.					
32651947	6	53	theme	novel	1186:1190	arg1	structure					1192:1200	a novel structure	1184:1200	a novel structure of fucoidan	1184:1212	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	54	theme	galactose	1294:1302	arg1	branches					1304:1311	α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches	1245:1311	branches	1304:1311	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	1	55	theme	algae	196:200	arg1	wall					182:185	the cell wall	173:185	the cell wall of brown algae	173:200	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	1	56	theme	polysaccharides	145:159	arg1	Fucoidans					103:111	Fucoidans	103:111	Fucoidans	103:111	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	1	56	theme	polysaccharides	145:159	arg1	drugs					262:266	potential drugs	252:266	potential drugs	252:266	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	1	56	theme	polysaccharides	145:159	arg1	class					127:131	a diverse class	117:131	a diverse class of sulfated polysaccharides integral to the cell wall of brown algae	117:200	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	1	56	theme	polysaccharides	145:159	arg1	they					243:246	they	243:246	they	243:246	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	6	57	theme	fucoidans	1161:1169	arg1	acid					1083:1086	galacturonic acid	1070:1086	galacturonic acid	1070:1086	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	57	theme	fucoidans	1161:1169	arg1	acid					1100:1103	glucuronic acid	1089:1103	glucuronic acid	1089:1103	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	57	theme	fucoidans	1161:1169	arg1	features					1141:1148	new structural features	1126:1148	new structural features of certain fucoidans	1126:1169	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	57	theme	fucoidans	1161:1169	arg1	O-acetylation					1109:1121	O-acetylation	1109:1121	O-acetylation	1109:1121	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	2	58	with	work	282:285	arg1	fucoidans					292:300	fucoidans	292:300	fucoidans	292:300	Standardized work with fucoidans is required for structure-function studies, but remains challenging since available fucoidan preparations are often contaminated with other algal compounds.					
32651947	0	59	theme	fucoidans	75:83	arg1	purification					13:24	Ion-exchange purification	0:24	Ion-exchange purification	0:24	Ion-exchange purification and structural characterization of five sulfated fucoidans from brown algae.					
32651947	0	59	theme	fucoidans	75:83	arg1	characterization					41:56	structural characterization	30:56	structural characterization	30:56	Ion-exchange purification and structural characterization of five sulfated fucoidans from brown algae.					
32651947	6	60	theme	β-1,3	1288:1292	arg1	branches					1304:1311	α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches	1245:1311	branches	1304:1311	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	1	61	theme	integral	161:168	arg1	Fucoidans					103:111	Fucoidans	103:111	Fucoidans	103:111	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	1	61	theme	integral	161:168	arg1	class					127:131	a diverse class	117:131	a diverse class of sulfated polysaccharides integral to the cell wall of brown algae	117:200	Fucoidans are a diverse class of sulfated polysaccharides integral to the cell wall of brown algae, and due to their various bioactivities, they are potential drugs.					
32651947	6	62	theme	new	1126:1128	arg1	acid					1083:1086	galacturonic acid	1070:1086	galacturonic acid	1070:1086	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	62	theme	new	1126:1128	arg1	acid					1100:1103	glucuronic acid	1089:1103	glucuronic acid	1089:1103	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	62	theme	new	1126:1128	arg1	features					1141:1148	new structural features	1126:1148	new structural features of certain fucoidans	1126:1169	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
32651947	6	62	theme	new	1126:1128	arg1	O-acetylation					1109:1121	O-acetylation	1109:1121	O-acetylation	1109:1121	By monomer composition, linkage analysis and NMR characterization, we identified galacturonic acid, glucuronic acid and O-acetylation as new structural features of certain fucoidans and provided a novel structure of fucoidan from Durvillaea potatorum with α-1,3-linked fucose backbone and β-1,6 and β-1,3 galactose branches.					
33648184	3	0	theme	starch	665:670	arg1	extraction					672:681	starch extraction	665:681	starch extraction	665:681	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	6	1	theme	minor	1187:1191	arg1	mannose					1204:1210	mannose	1204:1210	mannose	1204:1210	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	6	1	theme	minor	1187:1191	arg1	amounts					1193:1199	some minor amounts	1182:1199	some minor amounts of mannose and galactose in some fractions	1182:1242	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	6	1	theme	minor	1187:1191	arg1	galactose					1216:1224	galactose	1216:1224	galactose	1216:1224	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	7	2	theme	bamboo	1355:1360	arg1	species					1362:1368	all studied bamboo species	1343:1368	all studied bamboo species	1343:1368	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	2	3	dep	portions	376:383	arg1	portions					376:383	different portions	366:383	different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	366:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	3	dep	portions	376:383	arg1	top					405:407	top	405:407	top	405:407	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	3	dep	portions	376:383	arg1	middle					394:399	middle	394:399	middle	394:399	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	3	dep	portions	376:383	arg1	bottom					386:391	bottom	386:391	bottom	386:391	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	5	4	dep	%	1047:1047	arg1	to					1040:1041	to	1040:1041	to	1040:1041	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	5	5	theme	soluble	1059:1065	arg1	ones					1067:1070	the soluble ones	1055:1070	the soluble ones	1055:1070	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	4	6	theme	great	817:821	arg1	potential					823:831	great potential	817:831	great potential for commercial extraction	817:857	The hemicellulosic polysaccharide contents (35.4-41.5%) demonstrated great potential for commercial extraction and so, we extracted them with alkali and fractionated regarding their solubility in cold-water.					
33648184	2	7	theme	polysaccharide	594:607	arg1	parameters					535:544	their color parameters	523:544	their color parameters	523:544	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	7	theme	polysaccharide	594:607	arg1	characterization					609:624	hemicellulosic polysaccharide characterization	579:624	hemicellulosic polysaccharide characterization	579:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	6	8	from	fractions	1234:1242	arg1	mannose					1204:1210	mannose	1204:1210	mannose	1204:1210	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	6	8	from	fractions	1234:1242	arg1	amounts					1193:1199	some minor amounts	1182:1199	some minor amounts of mannose and galactose in some fractions	1182:1242	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	6	8	from	fractions	1234:1242	arg1	galactose					1216:1224	galactose	1216:1224	galactose	1216:1224	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	5	9	theme	5.2	1107:1109	arg1	%					1102:1102	%	1102:1102	%	1102:1102	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	2	10	theme	fibrous	435:441	arg1	fractions					443:451	the young bamboo culm fibrous fractions	413:451	the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	413:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	11	from	portions	376:383	arg1	asper					472:476	Dendrocalamus asper	458:476	Dendrocalamus asper	458:476	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	11	from	portions	376:383	arg1	tuldoides					487:495	Bambusa tuldoides	479:495	Bambusa tuldoides	479:495	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	11	from	portions	376:383	arg1	vulgaris					504:511	B. vulgaris	501:511	B. vulgaris	501:511	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	4	12	theme	commercial	837:846	arg1	extraction					848:857	commercial extraction	837:857	commercial extraction	837:857	The hemicellulosic polysaccharide contents (35.4-41.5%) demonstrated great potential for commercial extraction and so, we extracted them with alkali and fractionated regarding their solubility in cold-water.					
33648184	2	13	theme	hemicellulosic	579:592	arg1	parameters					535:544	their color parameters	523:544	their color parameters	523:544	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	13	theme	hemicellulosic	579:592	arg1	characterization					609:624	hemicellulosic polysaccharide characterization	579:624	hemicellulosic polysaccharide characterization	579:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	6	14	from	galactose	1216:1224	arg1	fractions					1234:1242	some fractions	1229:1242	some fractions	1229:1242	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	5	15	dep	obtained	1002:1009	arg1	fractions					1076:1084	KS fractions	1073:1084	KS fractions	1073:1084	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	5	15	dep	obtained	1002:1009	arg1	yields					1087:1092	yields	1087:1092	yields from 2.3% to 5.2%	1087:1110	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	2	16	theme	culm	430:433	arg1	fractions					443:451	the young bamboo culm fibrous fractions	413:451	the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	413:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	1	17	theme	supply	290:295	arg1	fibers					297:302	supply fibers	290:302	supply fibers	290:302	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	2	18	from	tuldoides	487:495	arg1	portions					376:383	different portions	366:383	different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	366:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	18	from	tuldoides	487:495	arg1	fractions					443:451	the young bamboo culm fibrous fractions	413:451	the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	413:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	18	from	tuldoides	487:495	arg1	middle					394:399	middle	394:399	middle	394:399	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	18	from	tuldoides	487:495	arg1	top					405:407	top	405:407	top	405:407	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	18	from	tuldoides	487:495	arg1	bottom					386:391	bottom	386:391	bottom	386:391	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	1	19	theme	dietary	224:230	arg1	fiber					232:236	dietary fiber	224:236	dietary fiber	224:236	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	2	20	theme	bamboo	423:428	arg1	fractions					443:451	the young bamboo culm fibrous fractions	413:451	the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	413:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	7	21	theme	studied	1347:1353	arg1	species					1362:1368	all studied bamboo species	1343:1368	all studied bamboo species	1343:1368	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	2	22	theme	physicochemical	547:561	arg1	parameters					535:544	their color parameters	523:544	their color parameters	523:544	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	22	theme	physicochemical	547:561	arg1	composition					563:573	physicochemical composition	547:573	physicochemical composition	547:573	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	0	23	theme	hemicellulosic	82:95	arg1	fibers					105:110	hemicellulosic dietary fibers	82:110	hemicellulosic dietary fibers	82:110	Young culm of Dendrocalamus asper, Bambusa tuldoides and B. Vulgaris as source of hemicellulosic dietary fibers for the food industry.					
33648184	7	24	theme	arabinoxylans	1308:1320	arg1	presence					1277:1284	the presence	1273:1284	the presence of neutral xylans and arabinoxylans	1273:1320	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	0	25	theme	dietary	97:103	arg1	fibers					105:110	hemicellulosic dietary fibers	82:110	hemicellulosic dietary fibers	82:110	Young culm of Dendrocalamus asper, Bambusa tuldoides and B. Vulgaris as source of hemicellulosic dietary fibers for the food industry.					
33648184	5	26	from	%	1047:1047	arg1	yields					1021:1026	higher yields	1014:1026	higher yields (from 21.2% to 38.5%) than the soluble ones	1014:1070	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	5	27	theme	2.3	1099:1101	arg1	%					1102:1102	%	1102:1102	%	1102:1102	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	2	28	from	vulgaris	504:511	arg1	portions					376:383	different portions	366:383	different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	366:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	28	from	vulgaris	504:511	arg1	fractions					443:451	the young bamboo culm fibrous fractions	413:451	the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	413:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	28	from	vulgaris	504:511	arg1	middle					394:399	middle	394:399	middle	394:399	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	28	from	vulgaris	504:511	arg1	top					405:407	top	405:407	top	405:407	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	28	from	vulgaris	504:511	arg1	bottom					386:391	bottom	386:391	bottom	386:391	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	7	29	theme	neutral	1289:1295	arg1	xylans					1297:1302	neutral xylans	1289:1302	neutral xylans	1289:1302	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	0	30	theme	Young	0:4	arg1	culm					6:9	Young culm	0:9	Young culm of Dendrocalamus asper, Bambusa	0:41	Young culm of Dendrocalamus asper, Bambusa tuldoides and B. Vulgaris as source of hemicellulosic dietary fibers for the food industry.					
33648184	1	31	theme	fiber	232:236	arg1	source					214:219	a source	212:219	a source of dietary fiber	212:236	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	1	31	theme	fiber	232:236	arg1	attention					178:186	economic attention	169:186	economic attention in the food industry	169:207	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	2	32	theme	color	529:533	arg1	parameters					535:544	their color parameters	523:544	their color parameters	523:544	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	32	theme	color	529:533	arg1	composition					563:573	physicochemical composition	547:573	physicochemical composition	547:573	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	32	theme	color	529:533	arg1	characterization					609:624	hemicellulosic polysaccharide characterization	579:624	hemicellulosic polysaccharide characterization	579:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	7	33	theme	biomaterials	1464:1475	arg1	production					1422:1431	the production	1418:1431	the production of xylooligosaccharides (XOS), biomaterials and biofuels	1418:1488	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	7	34	theme	xylans	1297:1302	arg1	presence					1277:1284	the presence	1273:1284	the presence of neutral xylans and arabinoxylans	1273:1320	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	7	35	theme	detailed	1250:1257	arg1	analysis					1263:1270	a detailed NMR analysis	1248:1270	a detailed NMR analysis	1248:1270	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	2	36	theme	different	366:374	arg1	portions					376:383	different portions	366:383	different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	366:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	36	theme	different	366:374	arg1	top					405:407	top	405:407	top	405:407	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	36	theme	different	366:374	arg1	middle					394:399	middle	394:399	middle	394:399	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	36	theme	different	366:374	arg1	bottom					386:391	bottom	386:391	bottom	386:391	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	3	37	theme	total	718:722	arg1	fiber					732:736	total dietary fiber	718:736	total dietary fiber (79-89%)	718:745	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	3	37	theme	total	718:722	arg1	%					744:744	79-89%	739:744	79-89%	739:744	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	5	38	from	%	1110:1110	arg1	fractions					1076:1084	KS fractions	1073:1084	KS fractions	1073:1084	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	5	38	from	%	1110:1110	arg1	yields					1087:1092	yields	1087:1092	yields from 2.3% to 5.2%	1087:1110	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	5	39	theme	KP	983:984	arg1	polysaccharides					966:980	Insoluble polysaccharides	956:980	Insoluble polysaccharides (KP fractions)	956:995	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	5	39	theme	KP	983:984	arg1	fractions					986:994	KP fractions	983:994	KP fractions	983:994	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	3	40	theme	high	702:705	arg1	fiber					732:736	total dietary fiber	718:736	total dietary fiber (79-89%)	718:745	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	3	40	theme	high	702:705	arg1	%					744:744	79-89%	739:744	79-89%	739:744	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	3	40	theme	high	702:705	arg1	amounts					707:713	high amounts	702:713	high amounts of total dietary fiber (79-89%)	702:745	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	0	41	theme	fibers	105:110	arg1	source					72:77	source	72:77	source of hemicellulosic dietary fibers for the food industry	72:132	Young culm of Dendrocalamus asper, Bambusa tuldoides and B. Vulgaris as source of hemicellulosic dietary fibers for the food industry.					
33648184	1	42	theme	economic	169:176	arg1	source					214:219	a source	212:219	a source of dietary fiber	212:236	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	1	42	theme	economic	169:176	arg1	attention					178:186	economic attention	169:186	economic attention in the food industry	169:207	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	0	43	theme	food	120:123	arg1	industry					125:132	the food industry	116:132	the food industry	116:132	Young culm of Dendrocalamus asper, Bambusa tuldoides and B. Vulgaris as source of hemicellulosic dietary fibers for the food industry.					
33648184	4	44	theme	polysaccharide	767:780	arg1	contents					782:789	The hemicellulosic polysaccharide contents	748:789	The hemicellulosic polysaccharide contents (35.4-41.5%)	748:802	The hemicellulosic polysaccharide contents (35.4-41.5%) demonstrated great potential for commercial extraction and so, we extracted them with alkali and fractionated regarding their solubility in cold-water.					
33648184	4	44	theme	polysaccharide	767:780	arg1	%					801:801	35.4-41.5%	792:801	35.4-41.5%	792:801	The hemicellulosic polysaccharide contents (35.4-41.5%) demonstrated great potential for commercial extraction and so, we extracted them with alkali and fractionated regarding their solubility in cold-water.					
33648184	1	45	theme	young	247:251	arg1	alternative					275:285	an alternative	272:285	an alternative to supply fibers to the market	272:316	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	1	45	theme	young	247:251	arg1	culm					260:263	the young bamboo culm	243:263	the young bamboo culm	243:263	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	2	46	theme	fractions	443:451	arg1	portions					376:383	different portions	366:383	different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	366:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	46	theme	fractions	443:451	arg1	top					405:407	top	405:407	top	405:407	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	46	theme	fractions	443:451	arg1	middle					394:399	middle	394:399	middle	394:399	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	46	theme	fractions	443:451	arg1	bottom					386:391	bottom	386:391	bottom	386:391	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	7	47	theme	food	1392:1395	arg1	products					1397:1404	food products	1392:1404	food products	1392:1404	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	4	48	theme	hemicellulosic	752:765	arg1	contents					782:789	The hemicellulosic polysaccharide contents	748:789	The hemicellulosic polysaccharide contents (35.4-41.5%)	748:802	The hemicellulosic polysaccharide contents (35.4-41.5%) demonstrated great potential for commercial extraction and so, we extracted them with alkali and fractionated regarding their solubility in cold-water.					
33648184	4	48	theme	hemicellulosic	752:765	arg1	%					801:801	35.4-41.5%	792:801	35.4-41.5%	792:801	The hemicellulosic polysaccharide contents (35.4-41.5%) demonstrated great potential for commercial extraction and so, we extracted them with alkali and fractionated regarding their solubility in cold-water.					
33648184	1	49	from	attention	178:186	arg1	industry					200:207	the food industry	191:207	the food industry	191:207	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	2	50	theme	young	417:421	arg1	fractions					443:451	the young bamboo culm fibrous fractions	413:451	the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	413:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	1	51	theme	bamboo	253:258	arg1	alternative					275:285	an alternative	272:285	an alternative to supply fibers to the market	272:316	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	1	51	theme	bamboo	253:258	arg1	culm					260:263	the young bamboo culm	243:263	the young bamboo culm	243:263	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	5	52	theme	%	1038:1038	arg1	%					1047:1047	21.2% to 38.5%	1034:1047	21.2% to 38.5%	1034:1047	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	5	53	dep	%	1102:1102	arg1	to					1104:1105	to	1104:1105	to	1104:1105	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	6	54	from	amounts	1193:1199	arg1	fractions					1234:1242	some fractions	1229:1242	some fractions	1229:1242	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	0	55	theme	Bambusa	35:41	arg1	culm					6:9	Young culm	0:9	Young culm of Dendrocalamus asper, Bambusa	0:41	Young culm of Dendrocalamus asper, Bambusa tuldoides and B. Vulgaris as source of hemicellulosic dietary fibers for the food industry.					
33648184	3	56	theme	dietary	724:730	arg1	fiber					732:736	total dietary fiber	718:736	total dietary fiber (79-89%)	718:745	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	3	56	theme	dietary	724:730	arg1	%					744:744	79-89%	739:744	79-89%	739:744	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	6	57	theme	mannose	1204:1210	arg1	mannose					1204:1210	mannose	1204:1210	mannose	1204:1210	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	6	57	theme	mannose	1204:1210	arg1	amounts					1193:1199	some minor amounts	1182:1199	some minor amounts of mannose and galactose in some fractions	1182:1242	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	6	57	theme	mannose	1204:1210	arg1	galactose					1216:1224	galactose	1216:1224	galactose	1216:1224	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	3	58	theme	Fibrous	627:633	arg1	fractions					635:643	Fibrous fractions	627:643	Fibrous fractions	627:643	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	3	59	theme	fiber	732:736	arg1	fiber					732:736	total dietary fiber	718:736	total dietary fiber (79-89%)	718:745	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	3	59	theme	fiber	732:736	arg1	%					744:744	79-89%	739:744	79-89%	739:744	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	3	59	theme	fiber	732:736	arg1	amounts					707:713	high amounts	702:713	high amounts of total dietary fiber (79-89%)	702:745	Fibrous fractions were obtained after starch extraction and results showed high amounts of total dietary fiber (79-89%).					
33648184	7	60	theme	xylooligosaccharides	1436:1455	arg1	production					1422:1431	the production	1418:1431	the production of xylooligosaccharides (XOS), biomaterials and biofuels	1418:1488	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	6	61	theme	galactose	1216:1224	arg1	mannose					1204:1210	mannose	1204:1210	mannose	1204:1210	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	6	61	theme	galactose	1216:1224	arg1	amounts					1193:1199	some minor amounts	1182:1199	some minor amounts of mannose and galactose in some fractions	1182:1242	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	6	61	theme	galactose	1216:1224	arg1	galactose					1216:1224	galactose	1216:1224	galactose	1216:1224	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	5	62	theme	higher	1014:1019	arg1	yields					1021:1026	higher yields	1014:1026	higher yields (from 21.2% to 38.5%) than the soluble ones	1014:1070	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	5	63	theme	Insoluble	956:964	arg1	polysaccharides					966:980	Insoluble polysaccharides	956:980	Insoluble polysaccharides (KP fractions)	956:995	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	5	63	theme	Insoluble	956:964	arg1	fractions					986:994	KP fractions	983:994	KP fractions	983:994	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	7	64	theme	biofuels	1481:1488	arg1	production					1422:1431	the production	1418:1431	the production of xylooligosaccharides (XOS), biomaterials and biofuels	1418:1488	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	7	65	located	observed	1331:1338	arg1	analysis					1263:1270	a detailed NMR analysis	1248:1270	a detailed NMR analysis	1248:1270	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	7	65	located	observed	1331:1338	arg1	species					1362:1368	all studied bamboo species	1343:1368	all studied bamboo species	1343:1368	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	7	65	located	observed	1331:1338	arg2	presence					1277:1284	the presence	1273:1284	the presence of neutral xylans and arabinoxylans	1273:1320	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	1	66	theme	food	195:198	arg1	industry					200:207	the food industry	191:207	the food industry	191:207	Bamboo is a grass that has gained economic attention in the food industry as a source of dietary fiber, and the young bamboo culm may be an alternative to supply fibers to the market.					
33648184	7	67	used	used	1384:1387	arg2	species					1362:1368	all studied bamboo species	1343:1368	all studied bamboo species	1343:1368	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	6	68	theme	Monosaccharide	1114:1127	arg1	composition					1129:1139	Monosaccharide composition	1114:1139	Monosaccharide composition	1114:1139	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	2	69	from	asper	472:476	arg1	portions					376:383	different portions	366:383	different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	366:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	69	from	asper	472:476	arg1	fractions					443:451	the young bamboo culm fibrous fractions	413:451	the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization	413:624	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	69	from	asper	472:476	arg1	middle					394:399	middle	394:399	middle	394:399	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	69	from	asper	472:476	arg1	top					405:407	top	405:407	top	405:407	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	2	69	from	asper	472:476	arg1	bottom					386:391	bottom	386:391	bottom	386:391	The objective was to evaluate and characterize different portions (bottom, middle and top) of the young bamboo culm fibrous fractions from Dendrocalamus asper, Bambusa tuldoides and B. vulgaris regarding their color parameters, physicochemical composition and hemicellulosic polysaccharide characterization.					
33648184	6	70	from	mannose	1204:1210	arg1	fractions					1234:1242	some fractions	1229:1242	some fractions	1229:1242	Monosaccharide composition showed mainly xylose and arabinose, with some minor amounts of mannose and galactose in some fractions.					
33648184	7	71	theme	NMR	1259:1261	arg1	analysis					1263:1270	a detailed NMR analysis	1248:1270	a detailed NMR analysis	1248:1270	In a detailed NMR analysis, the presence of neutral xylans and arabinoxylans could be observed in all studied bamboo species, which can be used in food products and also in the production of xylooligosaccharides (XOS), biomaterials and biofuels.					
33648184	5	72	theme	KS	1073:1074	arg1	fractions					1076:1084	KS fractions	1073:1084	KS fractions	1073:1084	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
33648184	5	72	theme	KS	1073:1074	arg1	yields					1087:1092	yields	1087:1092	yields from 2.3% to 5.2%	1087:1110	Insoluble polysaccharides (KP fractions) were obtained in higher yields (from 21.2% to 38.5%) than the soluble ones (KS fractions, yields from 2.3% to 5.2%).					
32414435	6	0	mod	modified	932:939	arg1	acids					881:885	propionic and butyric acids	859:885	propionic and butyric acids	859:885	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	0	mod	modified	932:939	arg3	enteropathogenic					956:971	enteropathogenic	956:971	enteropathogenic	956:971	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	0	mod	modified	932:939	arg3	Faecalibacterium					1091:1106	Faecalibacterium	1091:1106	Faecalibacterium	1091:1106	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	0	mod	modified	932:939	arg3	Lactobacillus					1044:1056	Lactobacillus	1044:1056	Lactobacillus	1044:1056	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	0	mod	modified	932:939	arg3	Prevotella					1076:1085	Prevotella	1076:1085	Prevotella	1076:1085	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	0	mod	modified	932:939	arg1	composition					911:921	the gut microbiota composition	892:921	the gut microbiota composition	892:921	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	0	mod	modified	932:939	arg3	bacteria					1109:1116	inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria	946:1116	bacteria	1109:1116	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	4	1	theme	fatty	694:698	arg1	SCFAs					707:711	SCFAs	707:711	SCFAs	707:711	During the fermentation, however, the total carbohydrate kept decreasing and short-chain fatty acids (SCFAs) maintained growing.					
32414435	4	1	theme	fatty	694:698	arg1	acids					700:704	short-chain fatty acids	682:704	short-chain fatty acids (SCFAs)	682:712	During the fermentation, however, the total carbohydrate kept decreasing and short-chain fatty acids (SCFAs) maintained growing.					
32414435	6	2	theme	beneficial	1025:1034	arg1	bacteria					1109:1116	inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria	946:1116	bacteria	1109:1116	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	2	theme	beneficial	1025:1034	arg1	Faecalibacterium					1091:1106	Faecalibacterium	1091:1106	Faecalibacterium	1091:1106	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	2	theme	beneficial	1025:1034	arg1	Prevotella					1076:1085	Prevotella	1076:1085	Prevotella	1076:1085	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	2	theme	beneficial	1025:1034	arg1	Lactobacillus					1044:1056	Lactobacillus	1044:1056	Lactobacillus	1044:1056	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	2	3	theme	TSP	342:344	arg1	fermentation					326:337	fermentation	326:337	fermentation	326:337	In this study, therefore, the dynamic digestion and fermentation of TSP in vitro were evaluated with the effect on gut microbiota composition estimated by high-throughput sequencing.					
32414435	2	3	theme	TSP	342:344	arg1	digestion					312:320	digestion	312:320	digestion	312:320	In this study, therefore, the dynamic digestion and fermentation of TSP in vitro were evaluated with the effect on gut microbiota composition estimated by high-throughput sequencing.					
32414435	1	4	theme	physico-chemical	230:245	arg1	properties					262:271	its excellent physico-chemical and biological properties	216:271	its excellent physico-chemical and biological properties	216:271	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	3	5	theme	digestions	501:510	arg1	results					461:467	The results	457:467	The results of gastric and small intestinal digestions	457:510	The results of gastric and small intestinal digestions showed the molecular weight of TSP kept stable with only small production of reducing sugar.					
32414435	6	6	contain	has	1134:1136	arg2	anti-inflammation					1195:1211	anti-inflammation	1195:1211	anti-inflammation	1195:1211	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	6	contain	has	1134:1136	arg1	TSP					1130:1132	TSP	1130:1132	TSP	1130:1132	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	6	contain	has	1134:1136	arg2	anti-obesity					1178:1189	anti-obesity	1178:1189	anti-obesity	1178:1189	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	6	contain	has	1134:1136	arg2	functions					1158:1166	potential prebiotic functions	1138:1166	potential prebiotic functions including anti-obesity and anti-inflammation	1138:1211	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	0	7	theme	microbiota	107:116	arg1	changes					118:124	gut microbiota changes	103:124	gut microbiota changes in vitro	103:133	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	6	8	theme	butyric	873:879	arg1	acids					881:885	propionic and butyric acids	859:885	propionic and butyric acids	859:885	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	4	9	theme	total	643:647	arg1	kept					662:665	the total carbohydrate kept decreasing	639:676	the total carbohydrate kept decreasing	639:676	During the fermentation, however, the total carbohydrate kept decreasing and short-chain fatty acids (SCFAs) maintained growing.					
32414435	1	10	theme	biological	251:260	arg1	properties					262:271	its excellent physico-chemical and biological properties	216:271	its excellent physico-chemical and biological properties	216:271	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	0	11	theme	gut	103:105	arg1	changes					118:124	gut microbiota changes	103:124	gut microbiota changes in vitro	103:133	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	0	12	from	Indigestibility	51:65	arg1	simulations					87:97	gastrointestinal simulations	70:97	gastrointestinal simulations	70:97	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	0	12	from	Indigestibility	51:65	arg1	vitro					129:133	vitro	129:133	vitro	129:133	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	3	13	theme	TSP	543:545	arg1	weight					533:538	the molecular weight	519:538	the molecular weight of TSP	519:545	The results of gastric and small intestinal digestions showed the molecular weight of TSP kept stable with only small production of reducing sugar.					
32414435	3	14	theme	gastric	472:478	arg1	digestions					501:510	gastric and small intestinal digestions	472:510	gastric and small intestinal digestions	472:510	The results of gastric and small intestinal digestions showed the molecular weight of TSP kept stable with only small production of reducing sugar.					
32414435	6	15	theme	propionic	859:867	arg1	acids					881:885	propionic and butyric acids	859:885	propionic and butyric acids	859:885	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	5	16	theme	non-branched	803:814	arg1	SCFAs					816:820	total and non-branched SCFAs	793:820	total and non-branched SCFAs	793:820	Compared to the control (distilled water), the contents of total and non-branched SCFAs were higher after fermentation, esp.					
32414435	3	17	theme	molecular	523:531	arg1	weight					533:538	the molecular weight	519:538	the molecular weight of TSP	519:545	The results of gastric and small intestinal digestions showed the molecular weight of TSP kept stable with only small production of reducing sugar.					
32414435	1	18	theme	seed	168:171	arg1	TSP					189:191	TSP	189:191	TSP	189:191	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	1	18	theme	seed	168:171	arg1	L.					164:165	Tamarindus indica L.	146:165	Tamarindus indica L.	146:165	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	1	18	theme	seed	168:171	arg1	polysaccharide					173:186	Tamarind (Tamarindus indica L.) seed polysaccharide	136:186	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP)	136:192	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	5	19	theme	distilled	759:767	arg1	control					750:756	the control	746:756	the control (distilled water)	746:774	Compared to the control (distilled water), the contents of total and non-branched SCFAs were higher after fermentation, esp.					
32414435	5	19	theme	distilled	759:767	arg1	water					769:773	distilled water	759:773	distilled water	759:773	Compared to the control (distilled water), the contents of total and non-branched SCFAs were higher after fermentation, esp.					
32414435	3	20	with	stable	552:557	arg1	production					575:584	only small production	564:584	only small production of reducing sugar	564:602	The results of gastric and small intestinal digestions showed the molecular weight of TSP kept stable with only small production of reducing sugar.					
32414435	2	21	theme	high-throughput	429:443	arg1	sequencing					445:454	high-throughput sequencing	429:454	high-throughput sequencing	429:454	In this study, therefore, the dynamic digestion and fermentation of TSP in vitro were evaluated with the effect on gut microbiota composition estimated by high-throughput sequencing.					
32414435	6	22	theme	prebiotic	1148:1156	arg1	anti-inflammation					1195:1211	anti-inflammation	1195:1211	anti-inflammation	1195:1211	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	22	theme	prebiotic	1148:1156	arg1	anti-obesity					1178:1189	anti-obesity	1178:1189	anti-obesity	1178:1189	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	22	theme	prebiotic	1148:1156	arg1	functions					1158:1166	potential prebiotic functions	1138:1166	potential prebiotic functions including anti-obesity and anti-inflammation	1138:1211	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	2	23	dep	digestion	312:320	arg1	the					300:302	the	300:302	the	300:302	In this study, therefore, the dynamic digestion and fermentation of TSP in vitro were evaluated with the effect on gut microbiota composition estimated by high-throughput sequencing.					
32414435	0	24	theme	Dynamic	0:6	arg1	digestion					8:16	Dynamic digestion	0:16	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.	0:134	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	6	25	theme	potential	1138:1146	arg1	anti-inflammation					1195:1211	anti-inflammation	1195:1211	anti-inflammation	1195:1211	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	25	theme	potential	1138:1146	arg1	anti-obesity					1178:1189	anti-obesity	1178:1189	anti-obesity	1178:1189	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	25	theme	potential	1138:1146	arg1	functions					1158:1166	potential prebiotic functions	1138:1166	potential prebiotic functions including anti-obesity and anti-inflammation	1138:1211	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	26	theme	genera	974:979	arg1	Escherichia-Shigell					981:999	genera Escherichia-Shigell	974:999	genera Escherichia-Shigell	974:999	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	4	27	theme	carbohydrate	649:660	arg1	kept					662:665	the total carbohydrate kept decreasing	639:676	the total carbohydrate kept decreasing	639:676	During the fermentation, however, the total carbohydrate kept decreasing and short-chain fatty acids (SCFAs) maintained growing.					
32414435	0	28	theme	tamarind	21:28	arg1	polysaccharide					35:48	tamarind seed polysaccharide	21:48	tamarind seed polysaccharide	21:48	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	3	29	theme	sugar	598:602	arg1	production					575:584	only small production	564:584	only small production of reducing sugar	564:602	The results of gastric and small intestinal digestions showed the molecular weight of TSP kept stable with only small production of reducing sugar.					
32414435	6	30	theme	epithelial	1248:1257	arg1	barrier					1259:1265	intestinal epithelial barrier	1237:1265	intestinal epithelial barrier	1237:1265	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	2	31	theme	microbiota	393:402	arg1	composition					404:414	gut microbiota composition	389:414	gut microbiota composition estimated by high-throughput sequencing	389:454	In this study, therefore, the dynamic digestion and fermentation of TSP in vitro were evaluated with the effect on gut microbiota composition estimated by high-throughput sequencing.					
32414435	5	32	theme	total	793:797	arg1	SCFAs					816:820	total and non-branched SCFAs	793:820	total and non-branched SCFAs	793:820	Compared to the control (distilled water), the contents of total and non-branched SCFAs were higher after fermentation, esp.					
32414435	4	33	theme	short-chain	682:692	arg1	SCFAs					707:711	SCFAs	707:711	SCFAs	707:711	During the fermentation, however, the total carbohydrate kept decreasing and short-chain fatty acids (SCFAs) maintained growing.					
32414435	4	33	theme	short-chain	682:692	arg1	acids					700:704	short-chain fatty acids	682:704	short-chain fatty acids (SCFAs)	682:712	During the fermentation, however, the total carbohydrate kept decreasing and short-chain fatty acids (SCFAs) maintained growing.					
32414435	2	34	theme	gut	389:391	arg1	composition					404:414	gut microbiota composition	389:414	gut microbiota composition estimated by high-throughput sequencing	389:454	In this study, therefore, the dynamic digestion and fermentation of TSP in vitro were evaluated with the effect on gut microbiota composition estimated by high-throughput sequencing.					
32414435	0	35	theme	polysaccharide	35:48	arg1	digestion					8:16	Dynamic digestion	0:16	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.	0:134	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	6	36	theme	intestinal	1237:1246	arg1	barrier					1259:1265	intestinal epithelial barrier	1237:1265	intestinal epithelial barrier	1237:1265	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	37	theme	promoted	1016:1023	arg1	bacteria					1109:1116	inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria	946:1116	bacteria	1109:1116	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	37	theme	promoted	1016:1023	arg1	Faecalibacterium					1091:1106	Faecalibacterium	1091:1106	Faecalibacterium	1091:1106	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	37	theme	promoted	1016:1023	arg1	Prevotella					1076:1085	Prevotella	1076:1085	Prevotella	1076:1085	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	37	theme	promoted	1016:1023	arg1	Lactobacillus					1044:1056	Lactobacillus	1044:1056	Lactobacillus	1044:1056	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	1	38	used	used	204:207	arg2	L.					164:165	Tamarindus indica L.	146:165	Tamarindus indica L.	146:165	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	1	38	used	used	204:207	arg2	TSP					189:191	TSP	189:191	TSP	189:191	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	1	38	used	used	204:207	arg2	polysaccharide					173:186	Tamarind (Tamarindus indica L.) seed polysaccharide	136:186	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP)	136:192	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	5	39	theme	SCFAs	816:820	arg1	contents					781:788	the contents	777:788	the contents of total and non-branched SCFAs	777:820	Compared to the control (distilled water), the contents of total and non-branched SCFAs were higher after fermentation, esp.					
32414435	5	39	theme	SCFAs	816:820	arg1	higher					827:832	higher	827:832	higher	827:832	Compared to the control (distilled water), the contents of total and non-branched SCFAs were higher after fermentation, esp.					
32414435	0	40	theme	seed	30:33	arg1	polysaccharide					35:48	tamarind seed polysaccharide	21:48	tamarind seed polysaccharide	21:48	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	3	41	theme	reducing	589:596	arg1	sugar					598:602	reducing sugar	589:602	reducing sugar	589:602	The results of gastric and small intestinal digestions showed the molecular weight of TSP kept stable with only small production of reducing sugar.					
32414435	3	42	theme	intestinal	490:499	arg1	digestions					501:510	gastric and small intestinal digestions	472:510	gastric and small intestinal digestions	472:510	The results of gastric and small intestinal digestions showed the molecular weight of TSP kept stable with only small production of reducing sugar.					
32414435	3	43	theme	small	569:573	arg1	production					575:584	only small production	564:584	only small production of reducing sugar	564:602	The results of gastric and small intestinal digestions showed the molecular weight of TSP kept stable with only small production of reducing sugar.					
32414435	6	44	theme	gut	896:898	arg1	composition					911:921	the gut microbiota composition	892:921	the gut microbiota composition	892:921	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	0	45	from	changes	118:124	arg1	simulations					87:97	gastrointestinal simulations	70:97	gastrointestinal simulations	70:97	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	0	45	from	changes	118:124	arg1	vitro					129:133	vitro	129:133	vitro	129:133	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	6	46	dep	bacteria	1109:1116	arg1	Lactobacillus					1044:1056	Lactobacillus	1044:1056	Lactobacillus	1044:1056	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	46	dep	bacteria	1109:1116	arg1	Faecalibacterium					1091:1106	Faecalibacterium	1091:1106	Faecalibacterium	1091:1106	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	46	dep	bacteria	1109:1116	arg1	Prevotella					1076:1085	Prevotella	1076:1085	Prevotella	1076:1085	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	46	dep	bacteria	1109:1116	arg1	bacteria					1109:1116	inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria	946:1116	bacteria	1109:1116	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	4	47	theme	decreasing	667:676	arg1	kept					662:665	the total carbohydrate kept decreasing	639:676	the total carbohydrate kept decreasing	639:676	During the fermentation, however, the total carbohydrate kept decreasing and short-chain fatty acids (SCFAs) maintained growing.					
32414435	1	48	theme	Tamarind	136:143	arg1	TSP					189:191	TSP	189:191	TSP	189:191	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	1	48	theme	Tamarind	136:143	arg1	L.					164:165	Tamarindus indica L.	146:165	Tamarindus indica L.	146:165	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	1	48	theme	Tamarind	136:143	arg1	polysaccharide					173:186	Tamarind (Tamarindus indica L.) seed polysaccharide	136:186	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP)	136:192	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	2	49	theme	dynamic	304:310	arg1	digestion					312:320	digestion	312:320	digestion	312:320	In this study, therefore, the dynamic digestion and fermentation of TSP in vitro were evaluated with the effect on gut microbiota composition estimated by high-throughput sequencing.					
32414435	0	50	theme	gastrointestinal	70:85	arg1	simulations					87:97	gastrointestinal simulations	70:97	gastrointestinal simulations	70:97	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	0	51	dep	digestion	8:16	arg1	changes					118:124	gut microbiota changes	103:124	gut microbiota changes in vitro	103:133	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	0	51	dep	digestion	8:16	arg1	Indigestibility					51:65	Indigestibility	51:65	Indigestibility in gastrointestinal simulations	51:97	Dynamic digestion of tamarind seed polysaccharide: Indigestibility in gastrointestinal simulations and gut microbiota changes in vitro.					
32414435	2	52	from	effect	379:384	arg1	composition					404:414	gut microbiota composition	389:414	gut microbiota composition estimated by high-throughput sequencing	389:454	In this study, therefore, the dynamic digestion and fermentation of TSP in vitro were evaluated with the effect on gut microbiota composition estimated by high-throughput sequencing.					
32414435	1	53	theme	Tamarindus	146:155	arg1	L.					164:165	Tamarindus indica L.	146:165	Tamarindus indica L.	146:165	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	1	53	theme	Tamarindus	146:155	arg1	polysaccharide					173:186	Tamarind (Tamarindus indica L.) seed polysaccharide	136:186	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP)	136:192	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	6	54	dep	enteropathogenic	956:971	arg1	Dorea					1005:1009	Dorea	1005:1009	Dorea	1005:1009	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	6	54	dep	enteropathogenic	956:971	arg1	Escherichia-Shigell					981:999	genera Escherichia-Shigell	974:999	genera Escherichia-Shigell	974:999	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	1	55	theme	indica	157:162	arg1	L.					164:165	Tamarindus indica L.	146:165	Tamarindus indica L.	146:165	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	1	55	theme	indica	157:162	arg1	polysaccharide					173:186	Tamarind (Tamarindus indica L.) seed polysaccharide	136:186	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP)	136:192	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
32414435	6	56	theme	microbiota	900:909	arg1	composition					911:921	the gut microbiota composition	892:921	the gut microbiota composition	892:921	propionic and butyric acids, and the gut microbiota composition was also modified with inhibited enteropathogenic (genera Escherichia-Shigell and Dorea) and promoted beneficial (genera Lactobacillus, Parabacteroides, Prevotella and Faecalibacterium) bacteria, suggesting TSP has potential prebiotic functions including anti-obesity and anti-inflammation, as well as maintaining intestinal epithelial barrier.					
32414435	1	57	theme	excellent	220:228	arg1	properties					262:271	its excellent physico-chemical and biological properties	216:271	its excellent physico-chemical and biological properties	216:271	Tamarind (Tamarindus indica L.) seed polysaccharide (TSP) is widely used due to its excellent physico-chemical and biological properties.					
34213104	10	0	theme	pressurized	2066:2076	arg1	electrochromatography					2088:2108	pressurized capillary electrochromatography	2066:2108	pressurized capillary electrochromatography for the separation and analysis of eight neutral monosaccharides	2066:2173	A novel method based on pressurized capillary electrochromatography for the separation and analysis of eight neutral monosaccharides has been established.					
34213104	17	1	theme	proposed	3614:3621	arg1	method					3623:3628	the proposed method	3610:3628	the proposed method	3610:3628	The results show that the proposed method has a good linear relationship and good repeatability.					
34213104	18	2	from	rhamnose	3852:3859	arg1	ratio					3886:3890	the molar ratio 1.00:0.16:0.14:0.07	3876:3910	the molar ratio 1.00:0.16:0.14:0.07	3876:3910	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	9	3	theme	optimal	1596:1602	arg1	conditions					1580:1589	the actual equipment conditions	1559:1589	the actual equipment conditions	1559:1589	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	3	theme	optimal	1596:1602	arg1	conditions					1615:1624	the optimal extraction conditions	1592:1624	the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power	1592:1879	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	15	4	dep	solution	3303:3310	arg1	pH					3325:3326	pH 4.1	3325:3330	pH 4.1	3325:3330	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	15	4	dep	solution	3303:3310	arg1	v/v					3320:3322	v/v	3320:3322	v/v	3320:3322	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	15	4	dep	solution	3303:3310	arg1	18:82					3313:3317	18:82	3313:3317	18:82	3313:3317	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	9	5	theme	ultrasonic	1828:1837	arg1	time					1850:1853	ultrasonic extraction time	1828:1853	ultrasonic extraction time	1828:1853	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	5	theme	ultrasonic	1828:1837	arg1	min					1859:1861	30 min	1856:1861	30 min	1856:1861	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	7	6	from	extraction	1237:1246	arg1	Pueraria					1272:1279	Pueraria	1272:1279	Pueraria	1272:1279	In this study, two kinds of Pueraria polysaccharides were extracted by an ultrasonic-assisted method, and response surface methodology was performed to explore the conditions for ultrasonic-assisted extraction of polysaccharides from Pueraria.					
34213104	19	7	from	glucose	3981:3987	arg1	ratio					4014:4018	the molar ratio 1.00:0.70	4004:4028	the molar ratio 1.00:0.70	4004:4028	The polysaccharides of Pueraria lobata Ohwi were mainly composed of glucose and mannose in the molar ratio 1.00:0.70.					
34213104	15	8	theme	experimental	3059:3070	arg1	follows					3102:3108	follows	3102:3108	follows	3102:3108	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	15	8	theme	experimental	3059:3070	arg1	conditions					3072:3081	The experimental conditions	3055:3081	The experimental conditions	3055:3081	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	18	9	from	fucose	3866:3871	arg1	ratio					3886:3890	the molar ratio 1.00:0.16:0.14:0.07	3876:3910	the molar ratio 1.00:0.16:0.14:0.07	3876:3910	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	8	10	theme	ultrasonic	1347:1356	arg1	time					1369:1372	ultrasonic extraction time	1347:1372	ultrasonic extraction time	1347:1372	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	8	10	theme	ultrasonic	1347:1356	arg1	factors					1314:1320	four factors	1309:1320	four factors	1309:1320	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	14	11	theme	surface	2728:2734	arg1	analysis					2736:2743	response surface analysis	2719:2743	response surface analysis	2719:2743	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	18	12	theme	actual	3735:3740	arg1	samples					3742:3748	the actual samples	3731:3748	the actual samples	3731:3748	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	20	13	theme	novel	4053:4057	arg1	method					4059:4064	a novel method	4051:4064	a novel method for the rapid and efficient separation and detection of neutral monosaccharides	4051:4144	This study provides a novel method for the rapid and efficient separation and detection of neutral monosaccharides, and serves as a reference for analyzing the monosaccharide composition of Pueraria polysaccharides.					
34213104	1	14	contain	possess	182:188	arg2	activities					201:210	biological activities	190:210	biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities	190:295	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	1	14	contain	possess	182:188	arg1	polysaccharides					146:160	Pueraria polysaccharides	137:160	Pueraria polysaccharides	137:160	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	1	14	contain	possess	182:188	arg2	activities					286:295	immunity boosting activities	268:295	immunity boosting activities	268:295	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	1	14	contain	possess	182:188	arg2	bacteriostasis					220:233	bacteriostasis	220:233	bacteriostasis	220:233	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	17	15	theme	good	3636:3639	arg1	relationship					3648:3659	a good linear relationship	3634:3659	a good linear relationship	3634:3659	The results show that the proposed method has a good linear relationship and good repeatability.					
34213104	9	16	theme	Pueraria	1630:1637	arg1	polysaccharides					1639:1653	Pueraria polysaccharides	1630:1653	Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power	1630:1879	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	13	17	dep	detection	2619:2627	arg1	the					2615:2617	the	2615:2617	the	2615:2617	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	15	18	theme	derivatives	3157:3167	arg1	separation					3114:3123	the separation	3110:3123	the separation of eight neutral monosaccharide derivatives	3110:3167	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	15	18	theme	derivatives	3157:3167	arg1	phase					3347:3351	the mobile phase	3336:3351	the mobile phase	3336:3351	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	4	19	theme	ultraviolet	748:758	arg1	region					760:765	the ultraviolet region	744:765	the ultraviolet region	744:765	Direct detection of saccharides is difficult because they are strongly polar and do not show absorption in the ultraviolet region.					
34213104	14	20	from	kinds	2910:2914	arg1	polysaccharides					2881:2895	polysaccharides	2881:2895	polysaccharides from the two kinds of Pueraria	2881:2926	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	14	20	from	kinds	2910:2914	arg1	rate					2873:2876	the extraction rate	2858:2876	the extraction rate of polysaccharides from the two kinds of Pueraria	2858:2926	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	8	21	theme	ultrasonic	1375:1384	arg1	temperature					1397:1407	ultrasonic extraction temperature	1375:1407	ultrasonic extraction temperature	1375:1407	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	8	21	theme	ultrasonic	1375:1384	arg1	factors					1314:1320	four factors	1309:1320	four factors	1309:1320	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	14	22	theme	Pueraria	2919:2926	arg1	kinds					2910:2914	the two kinds	2902:2914	the two kinds of Pueraria	2902:2926	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	11	23	theme	derivatization	2283:2296	arg1	method					2298:2303	the 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization method	2237:2303	the 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization method	2237:2303	The monosaccharides were derivatized by the 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization method.					
34213104	15	24	theme	neutral	3134:3140	arg1	derivatives					3157:3167	eight neutral monosaccharide derivatives	3128:3167	eight neutral monosaccharide derivatives	3128:3167	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	8	25	theme	extraction	1439:1448	arg1	rate					1450:1453	the extraction rate	1435:1453	the extraction rate of the polysaccharides	1435:1476	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	13	26	theme	pressurized	2527:2537	arg1	method					2593:2598	the established pressurized capillary electrochromatography-ultraviolet detection method	2511:2598	the established pressurized capillary electrochromatography-ultraviolet detection method	2511:2598	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	11	27	theme	1-phenyl-3-methyl-5-pyrazolone	2241:2270	arg1	method					2298:2303	the 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization method	2237:2303	the 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization method	2237:2303	The monosaccharides were derivatized by the 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization method.					
34213104	16	28	theme	chromatography	3565:3578	arg1	method					3580:3585	the traditional liquid chromatography method	3542:3585	the traditional liquid chromatography method	3542:3585	The separation and detection speeds and the column efficiency achieved with this method were much better than those obtained with the traditional liquid chromatography method.					
34213104	15	29	theme	C18	3229:3231	arg1	column					3243:3248	a Halo-2.7 μm core-shell C18 capillary column	3204:3248	a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1)	3204:3331	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	15	30	theme	acetate	3287:3293	arg1	solution					3303:3310	acetonitrile-50 mmol/L ammonium acetate aqueous solution	3255:3310	acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1)	3255:3331	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	7	31	theme	ultrasonic-assisted	1217:1235	arg1	extraction					1237:1246	ultrasonic-assisted extraction	1217:1246	ultrasonic-assisted extraction of polysaccharides from Pueraria	1217:1279	In this study, two kinds of Pueraria polysaccharides were extracted by an ultrasonic-assisted method, and response surface methodology was performed to explore the conditions for ultrasonic-assisted extraction of polysaccharides from Pueraria.					
34213104	13	32	theme	electrochromatography-ultraviolet	2549:2581	arg1	method					2593:2598	the established pressurized capillary electrochromatography-ultraviolet detection method	2511:2598	the established pressurized capillary electrochromatography-ultraviolet detection method	2511:2598	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	15	33	theme	μm	3215:3216	arg1	column					3243:3248	a Halo-2.7 μm core-shell C18 capillary column	3204:3248	a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1)	3204:3331	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	18	34	theme	molar	3880:3884	arg1	ratio					3886:3890	the molar ratio 1.00:0.16:0.14:0.07	3876:3910	the molar ratio 1.00:0.16:0.14:0.07	3876:3910	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	8	35	theme	polysaccharides	1462:1476	arg1	rate					1450:1453	the extraction rate	1435:1453	the extraction rate of the polysaccharides	1435:1476	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	10	36	theme	monosaccharides	2159:2173	arg1	analysis					2133:2140	analysis	2133:2140	analysis	2133:2140	A novel method based on pressurized capillary electrochromatography for the separation and analysis of eight neutral monosaccharides has been established.					
34213104	10	36	theme	monosaccharides	2159:2173	arg1	separation					2118:2127	separation	2118:2127	separation	2118:2127	A novel method based on pressurized capillary electrochromatography for the separation and analysis of eight neutral monosaccharides has been established.					
34213104	7	37	theme	surface	1153:1159	arg1	methodology					1161:1171	response surface methodology	1144:1171	response surface methodology	1144:1171	In this study, two kinds of Pueraria polysaccharides were extracted by an ultrasonic-assisted method, and response surface methodology was performed to explore the conditions for ultrasonic-assisted extraction of polysaccharides from Pueraria.					
34213104	9	38	theme	extraction	1689:1698	arg1	temperature					1700:1710	ultrasonic extraction temperature	1678:1710	ultrasonic extraction temperature	1678:1710	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	38	theme	extraction	1689:1698	arg1	90℃					1713:1715	90℃	1713:1715	90℃	1713:1715	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	2	39	from	structure	319:327	arg1	polysaccharides					382:396	these polysaccharides	376:396	these polysaccharides	376:396	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	14	40	theme	polysaccharides	2881:2895	arg1	rate					2873:2876	the extraction rate	2858:2876	the extraction rate of polysaccharides from the two kinds of Pueraria	2858:2926	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	2	41	from	composition	330:340	arg1	polysaccharides					382:396	these polysaccharides	376:396	these polysaccharides	376:396	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	15	42	theme	acetonitrile-50	3255:3269	arg1	mmol/L					3271:3276	mmol/L	3271:3276	mmol/L	3271:3276	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	2	43	dep	structure	319:327	arg1	the					315:317	the	315:317	the	315:317	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	5	44	theme	liquid	835:840	arg1	methods					798:804	the commonly used methods	780:804	the commonly used methods for saccharide detection	780:829	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	5	44	theme	liquid	835:840	arg1	detection					869:877	liquid chromatography-ultraviolet detection	835:877	liquid chromatography-ultraviolet detection	835:877	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	15	45	theme	voltage	3394:3400	arg1	kV					3408:3409	an applied voltage of-20 kV	3383:3409	an applied voltage of-20 kV	3383:3409	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	10	46	dep	separation	2118:2127	arg1	the					2114:2116	the	2114:2116	the	2114:2116	A novel method based on pressurized capillary electrochromatography for the separation and analysis of eight neutral monosaccharides has been established.					
34213104	20	47	theme	efficient	4084:4092	arg1	separation					4094:4103	rapid and efficient separation	4074:4103	rapid and efficient separation	4074:4103	This study provides a novel method for the rapid and efficient separation and detection of neutral monosaccharides, and serves as a reference for analyzing the monosaccharide composition of Pueraria polysaccharides.					
34213104	0	48	theme	pressurized	91:101	arg1	electrochromatography					113:133	pressurized capillary electrochromatography	91:133	pressurized capillary electrochromatography	91:133	[High-efficiency separation and analysis of monosaccharides in Pueraria polysaccharides by pressurized capillary electrochromatography].					
34213104	8	49	from	effects	1298:1304	arg1	rate					1450:1453	the extraction rate	1435:1453	the extraction rate of the polysaccharides	1435:1476	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	9	50	theme	quadratic	1924:1932	arg1	equation					1945:1952	the multiple quadratic regression equation	1911:1952	the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides	1911:2023	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	51	dep	temperature	1700:1710	arg1	power					1875:1879	ultrasonic power	1864:1879	ultrasonic power	1864:1879	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	51	dep	temperature	1700:1710	arg1	min					1859:1861	30 min	1856:1861	30 min	1856:1861	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	51	dep	temperature	1700:1710	arg1	ratio					1788:1792	liquid-solid ratio	1775:1792	liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g	1775:1825	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	51	dep	temperature	1700:1710	arg1	time					1850:1853	ultrasonic extraction time	1828:1853	ultrasonic extraction time	1828:1853	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	51	dep	temperature	1700:1710	arg1	ratio					1731:1735	liquid-solid ratio	1718:1735	liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g	1718:1772	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	13	52	theme	polysaccharide	2680:2693	arg1	samples					2695:2701	actual Pueraria polysaccharide samples	2664:2701	actual Pueraria polysaccharide samples	2664:2701	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	15	53	from	min	3197:3199	arg1	column					3243:3248	a Halo-2.7 μm core-shell C18 capillary column	3204:3248	a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1)	3204:3331	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	12	54	theme	phase	2474:2478	arg1	ratio					2480:2484	mobile phase ratio	2467:2484	mobile phase ratio	2467:2484	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	15	55	theme	mmol/L	3271:3276	arg1	acetate					3287:3293	acetonitrile-50 mmol/L ammonium acetate	3255:3293	acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1)	3255:3331	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	12	56	theme	mobile	2467:2472	arg1	ratio					2480:2484	mobile phase ratio	2467:2484	mobile phase ratio	2467:2484	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	2	57	theme	biological	443:452	arg1	activities					454:463	biological activities	443:463	biological activities	443:463	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	7	58	theme	polysaccharides	1075:1089	arg1	kinds					1057:1061	two kinds	1053:1061	two kinds of Pueraria polysaccharides	1053:1089	In this study, two kinds of Pueraria polysaccharides were extracted by an ultrasonic-assisted method, and response surface methodology was performed to explore the conditions for ultrasonic-assisted extraction of polysaccharides from Pueraria.					
34213104	9	59	theme	Benth	1759:1763	arg1	ratio					1731:1735	liquid-solid ratio	1718:1735	liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g	1718:1772	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	14	60	theme	ultrasonic	3017:3026	arg1	power					3028:3032	ultrasonic power	3017:3032	ultrasonic power	3017:3032	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	16	61	theme	traditional	3546:3556	arg1	method					3580:3585	the traditional liquid chromatography method	3542:3585	the traditional liquid chromatography method	3542:3585	The separation and detection speeds and the column efficiency achieved with this method were much better than those obtained with the traditional liquid chromatography method.					
34213104	0	62	theme	[High-efficiency	0:15	arg1	separation					17:26	[High-efficiency separation	0:26	[High-efficiency separation	0:26	[High-efficiency separation and analysis of monosaccharides in Pueraria polysaccharides by pressurized capillary electrochromatography].					
34213104	9	63	theme	optimal	1510:1516	arg1	conditions					1518:1527	the optimal conditions	1506:1527	the optimal conditions predicted by the software	1506:1553	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	13	64	theme	actual	2664:2669	arg1	samples					2695:2701	actual Pueraria polysaccharide samples	2664:2701	actual Pueraria polysaccharide samples	2664:2701	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	14	65	theme	time	3008:3011	arg1	weak					3049:3052	weak	3049:3052	weak	3049:3052	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	14	65	theme	time	3008:3011	arg1	influence					2969:2977	the influence	2965:2977	the influence of the ultrasonic extraction time and ultrasonic power	2965:3032	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	9	66	theme	factors	1966:1972	arg1	equation					1945:1952	the multiple quadratic regression equation	1911:1952	the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides	1911:2023	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	12	67	theme	chromatographic	2439:2453	arg1	column					2455:2460	chromatographic column	2439:2460	chromatographic column	2439:2460	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	15	68	from	nm	3374:3375	arg1	detection					3357:3365	detection	3357:3365	detection at 250 nm under an applied voltage of-20 kV	3357:3409	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	6	69	theme	microseparation	1010:1024	arg1	technology					1026:1035	a high-efficiency microseparation technology	992:1035	a high-efficiency microseparation technology	992:1035	Pressurized capillary electrochromatography is a high-efficiency microseparation technology.					
34213104	6	69	theme	microseparation	1010:1024	arg1	electrochromatography					967:987	Pressurized capillary electrochromatography	945:987	Pressurized capillary electrochromatography	945:987	Pressurized capillary electrochromatography is a high-efficiency microseparation technology.					
34213104	1	70	theme	Pueraria	137:144	arg1	polysaccharides					146:160	Pueraria polysaccharides	137:160	Pueraria polysaccharides	137:160	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	14	71	theme	ultrasonic	2986:2995	arg1	time					3008:3011	the ultrasonic extraction time	2982:3011	the ultrasonic extraction time	2982:3011	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	9	72	dep	polysaccharides	1639:1653	arg1	temperature					1700:1710	ultrasonic extraction temperature	1678:1710	ultrasonic extraction temperature	1678:1710	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	72	dep	polysaccharides	1639:1653	arg1	90℃					1713:1715	90℃	1713:1715	90℃	1713:1715	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	5	73	theme	gas	880:882	arg1	detection					911:919	gas chromatography-ultraviolet detection	880:919	gas chromatography-ultraviolet detection	880:919	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	14	74	theme	extraction	2805:2814	arg1	temperature					2816:2826	ultrasonic extraction temperature	2794:2826	ultrasonic extraction temperature	2794:2826	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	15	75	theme	applied	3386:3392	arg1	kV					3408:3409	an applied voltage of-20 kV	3383:3409	an applied voltage of-20 kV	3383:3409	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	20	76	theme	neutral	4122:4128	arg1	monosaccharides					4130:4144	neutral monosaccharides	4122:4144	neutral monosaccharides	4122:4144	This study provides a novel method for the rapid and efficient separation and detection of neutral monosaccharides, and serves as a reference for analyzing the monosaccharide composition of Pueraria polysaccharides.					
34213104	3	77	theme	Pueraria	491:498	arg1	polysaccharides					500:514	Pueraria polysaccharides	491:514	Pueraria polysaccharides	491:514	Therefore, extraction of Pueraria polysaccharides and determination of the monosaccharide composition are of great significance for activity analysis and quality control.					
34213104	19	78	from	mannose	3993:3999	arg1	ratio					4014:4018	the molar ratio 1.00:0.70	4004:4028	the molar ratio 1.00:0.70	4004:4028	The polysaccharides of Pueraria lobata Ohwi were mainly composed of glucose and mannose in the molar ratio 1.00:0.70.					
34213104	13	79	theme	kinds	2655:2659	arg1	detection					2619:2627	detection	2619:2627	detection	2619:2627	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	13	79	theme	kinds	2655:2659	arg1	identification					2633:2646	identification	2633:2646	identification	2633:2646	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	9	80	theme	Pueraria	2000:2007	arg1	polysaccharides					2009:2023	Pueraria polysaccharides	2000:2023	Pueraria polysaccharides	2000:2023	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	10	81	theme	novel	2044:2048	arg1	method					2050:2055	A novel method	2042:2055	A novel method based on pressurized capillary electrochromatography for the separation and analysis of eight neutral monosaccharides	2042:2173	A novel method based on pressurized capillary electrochromatography for the separation and analysis of eight neutral monosaccharides has been established.					
34213104	3	82	theme	monosaccharide	541:554	arg1	composition					556:566	the monosaccharide composition	537:566	the monosaccharide composition	537:566	Therefore, extraction of Pueraria polysaccharides and determination of the monosaccharide composition are of great significance for activity analysis and quality control.					
34213104	9	83	theme	actual	1563:1568	arg1	conditions					1580:1589	the actual equipment conditions	1559:1589	the actual equipment conditions	1559:1589	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	83	theme	actual	1563:1568	arg1	W.					1886:1887	W.	1886:1887	W.	1886:1887	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	83	theme	actual	1563:1568	arg1	conditions					1615:1624	the optimal extraction conditions	1592:1624	the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power	1592:1879	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	6	84	theme	Pressurized	945:955	arg1	technology					1026:1035	a high-efficiency microseparation technology	992:1035	a high-efficiency microseparation technology	992:1035	Pressurized capillary electrochromatography is a high-efficiency microseparation technology.					
34213104	6	84	theme	Pressurized	945:955	arg1	electrochromatography					967:987	Pressurized capillary electrochromatography	945:987	Pressurized capillary electrochromatography	945:987	Pressurized capillary electrochromatography is a high-efficiency microseparation technology.					
34213104	9	85	theme	Ohwi	1813:1816	arg1	ratio					1788:1792	liquid-solid ratio	1775:1792	liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g	1775:1825	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	18	86	theme	Pueraria	3785:3792	arg1	Benth					3804:3808	Pueraria thomsonii Benth	3785:3808	Pueraria thomsonii Benth	3785:3808	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	1	87	dep	anti-oxidative	236:249	arg1	anti-tumor					252:261	anti-tumor	252:261	anti-tumor	252:261	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	2	88	theme	different	410:418	arg1	structures					428:437	different spatial structures	410:437	different spatial structures	410:437	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	1	89	theme	boosting	277:284	arg1	activities					286:295	immunity boosting activities	268:295	immunity boosting activities	268:295	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	5	90	theme	chromatography-ultraviolet	884:909	arg1	detection					911:919	gas chromatography-ultraviolet detection	880:919	gas chromatography-ultraviolet detection	880:919	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	20	91	theme	Pueraria	4221:4228	arg1	polysaccharides					4230:4244	Pueraria polysaccharides	4221:4244	Pueraria polysaccharides	4221:4244	This study provides a novel method for the rapid and efficient separation and detection of neutral monosaccharides, and serves as a reference for analyzing the monosaccharide composition of Pueraria polysaccharides.					
34213104	5	92	theme	chromatography-ultraviolet	842:867	arg1	methods					798:804	the commonly used methods	780:804	the commonly used methods for saccharide detection	780:829	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	5	92	theme	chromatography-ultraviolet	842:867	arg1	detection					869:877	liquid chromatography-ultraviolet detection	835:877	liquid chromatography-ultraviolet detection	835:877	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	3	93	theme	great	575:579	arg1	significance					581:592	great significance	575:592	great significance	575:592	Therefore, extraction of Pueraria polysaccharides and determination of the monosaccharide composition are of great significance for activity analysis and quality control.					
34213104	4	94	theme	saccharides	657:667	arg1	detection					644:652	Direct detection	637:652	Direct detection of saccharides	637:667	Direct detection of saccharides is difficult because they are strongly polar and do not show absorption in the ultraviolet region.					
34213104	3	95	theme	quality	620:626	arg1	control					628:634	quality control	620:634	quality control	620:634	Therefore, extraction of Pueraria polysaccharides and determination of the monosaccharide composition are of great significance for activity analysis and quality control.					
34213104	9	96	theme	extraction	1604:1613	arg1	conditions					1580:1589	the actual equipment conditions	1559:1589	the actual equipment conditions	1559:1589	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	96	theme	extraction	1604:1613	arg1	conditions					1615:1624	the optimal extraction conditions	1592:1624	the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power	1592:1879	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	8	97	theme	liquid-solid	1327:1338	arg1	ratio					1340:1344	the liquid-solid ratio	1323:1344	the liquid-solid ratio	1323:1344	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	8	97	theme	liquid-solid	1327:1338	arg1	factors					1314:1320	four factors	1309:1320	four factors	1309:1320	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	14	98	theme	analysis	2736:2743	arg1	results					2708:2714	The results	2704:2714	The results of response surface analysis	2704:2743	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	9	99	theme	extraction	1839:1848	arg1	time					1850:1853	ultrasonic extraction time	1828:1853	ultrasonic extraction time	1828:1853	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	99	theme	extraction	1839:1848	arg1	min					1859:1861	30 min	1856:1861	30 min	1856:1861	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	100	theme	liquid-solid	1718:1729	arg1	ratio					1731:1735	liquid-solid ratio	1718:1735	liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g	1718:1772	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	13	101	theme	detection	2583:2591	arg1	method					2593:2598	the established pressurized capillary electrochromatography-ultraviolet detection method	2511:2598	the established pressurized capillary electrochromatography-ultraviolet detection method	2511:2598	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	19	102	theme	molar	4008:4012	arg1	ratio					4014:4018	the molar ratio 1.00:0.70	4004:4028	the molar ratio 1.00:0.70	4004:4028	The polysaccharides of Pueraria lobata Ohwi were mainly composed of glucose and mannose in the molar ratio 1.00:0.70.					
34213104	14	103	theme	response	2719:2726	arg1	analysis					2736:2743	response surface analysis	2719:2743	response surface analysis	2719:2743	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	17	104	contain	has	3630:3632	arg2	relationship					3648:3659	a good linear relationship	3634:3659	a good linear relationship	3634:3659	The results show that the proposed method has a good linear relationship and good repeatability.					
34213104	17	104	contain	has	3630:3632	arg1	method					3623:3628	the proposed method	3610:3628	the proposed method	3610:3628	The results show that the proposed method has a good linear relationship and good repeatability.					
34213104	17	104	contain	has	3630:3632	arg2	repeatability					3670:3682	good repeatability	3665:3682	good repeatability	3665:3682	The results show that the proposed method has a good linear relationship and good repeatability.					
34213104	14	105	from	rate	2873:2876	arg1	kinds					2910:2914	the two kinds	2902:2914	the two kinds of Pueraria	2902:2926	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	8	106	theme	ultrasonic	1414:1423	arg1	power					1425:1429	ultrasonic power	1414:1429	ultrasonic power	1414:1429	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	8	106	theme	ultrasonic	1414:1423	arg1	factors					1314:1320	four factors	1309:1320	four factors	1309:1320	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	7	107	theme	polysaccharides	1251:1265	arg1	extraction					1237:1246	ultrasonic-assisted extraction	1217:1246	ultrasonic-assisted extraction of polysaccharides from Pueraria	1217:1279	In this study, two kinds of Pueraria polysaccharides were extracted by an ultrasonic-assisted method, and response surface methodology was performed to explore the conditions for ultrasonic-assisted extraction of polysaccharides from Pueraria.					
34213104	17	108	theme	linear	3641:3646	arg1	relationship					3648:3659	a good linear relationship	3634:3659	a good linear relationship	3634:3659	The results show that the proposed method has a good linear relationship and good repeatability.					
34213104	4	109	from	absorption	730:739	arg1	region					760:765	the ultraviolet region	744:765	the ultraviolet region	744:765	Direct detection of saccharides is difficult because they are strongly polar and do not show absorption in the ultraviolet region.					
34213104	8	110	theme	extraction	1358:1367	arg1	time					1369:1372	ultrasonic extraction time	1347:1372	ultrasonic extraction time	1347:1372	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	8	110	theme	extraction	1358:1367	arg1	factors					1314:1320	four factors	1309:1320	four factors	1309:1320	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	20	111	dep	separation	4094:4103	arg1	the					4070:4072	the	4070:4072	the	4070:4072	This study provides a novel method for the rapid and efficient separation and detection of neutral monosaccharides, and serves as a reference for analyzing the monosaccharide composition of Pueraria polysaccharides.					
34213104	15	112	with	column	3243:3248	arg1	solution					3303:3310	acetonitrile-50 mmol/L ammonium acetate aqueous solution	3255:3310	acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1)	3255:3331	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	14	113	contain	had	2828:2830	arg1	temperature					2816:2826	ultrasonic extraction temperature	2794:2826	ultrasonic extraction temperature	2794:2826	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	14	113	contain	had	2828:2830	arg2	influence					2845:2853	the greatest influence	2832:2853	the greatest influence	2832:2853	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	1	114	theme	anti-oxidative	236:249	arg1	bacteriostasis					220:233	bacteriostasis	220:233	bacteriostasis	220:233	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	9	115	theme	ultrasonic	1864:1873	arg1	power					1875:1879	ultrasonic power	1864:1879	ultrasonic power	1864:1879	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	15	116	theme	monosaccharide	3142:3155	arg1	derivatives					3157:3167	eight neutral monosaccharide derivatives	3128:3167	eight neutral monosaccharide derivatives	3128:3167	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	13	117	theme	capillary	2539:2547	arg1	method					2593:2598	the established pressurized capillary electrochromatography-ultraviolet detection method	2511:2598	the established pressurized capillary electrochromatography-ultraviolet detection method	2511:2598	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	8	118	theme	extraction	1386:1395	arg1	temperature					1397:1407	ultrasonic extraction temperature	1375:1407	ultrasonic extraction temperature	1375:1407	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	8	118	theme	extraction	1386:1395	arg1	factors					1314:1320	four factors	1309:1320	four factors	1309:1320	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	15	119	theme	mobile	3340:3345	arg1	separation					3114:3123	the separation	3110:3123	the separation of eight neutral monosaccharide derivatives	3110:3167	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	15	119	theme	mobile	3340:3345	arg1	phase					3347:3351	the mobile phase	3336:3351	the mobile phase	3336:3351	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	11	120	theme	pre-column	2272:2281	arg1	method					2298:2303	the 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization method	2237:2303	the 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization method	2237:2303	The monosaccharides were derivatized by the 1-phenyl-3-methyl-5-pyrazolone pre-column derivatization method.					
34213104	15	121	theme	capillary	3233:3241	arg1	column					3243:3248	a Halo-2.7 μm core-shell C18 capillary column	3204:3248	a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1)	3204:3331	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	20	122	theme	rapid	4074:4078	arg1	separation					4094:4103	rapid and efficient separation	4074:4103	rapid and efficient separation	4074:4103	This study provides a novel method for the rapid and efficient separation and detection of neutral monosaccharides, and serves as a reference for analyzing the monosaccharide composition of Pueraria polysaccharides.					
34213104	16	123	theme	column	3456:3461	arg1	better					3510:3515	better	3510:3515	better	3510:3515	The separation and detection speeds and the column efficiency achieved with this method were much better than those obtained with the traditional liquid chromatography method.					
34213104	16	123	theme	column	3456:3461	arg1	efficiency					3463:3472	the column efficiency	3452:3472	the column efficiency achieved with this method	3452:3498	The separation and detection speeds and the column efficiency achieved with this method were much better than those obtained with the traditional liquid chromatography method.					
34213104	9	124	theme	ultrasonic	1678:1687	arg1	temperature					1700:1710	ultrasonic extraction temperature	1678:1710	ultrasonic extraction temperature	1678:1710	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	124	theme	ultrasonic	1678:1687	arg1	90℃					1713:1715	90℃	1713:1715	90℃	1713:1715	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	15	125	theme	core-shell	3218:3227	arg1	column					3243:3248	a Halo-2.7 μm core-shell C18 capillary column	3204:3248	a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1)	3204:3331	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	10	126	theme	neutral	2151:2157	arg1	monosaccharides					2159:2173	eight neutral monosaccharides	2145:2173	eight neutral monosaccharides	2145:2173	A novel method based on pressurized capillary electrochromatography for the separation and analysis of eight neutral monosaccharides has been established.					
34213104	15	127	theme	of-20	3402:3406	arg1	kV					3408:3409	an applied voltage of-20 kV	3383:3409	an applied voltage of-20 kV	3383:3409	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	9	128	theme	data	1897:1900	arg1	fitting					1902:1908	data fitting	1897:1908	data fitting	1897:1908	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	12	129	theme	separation	2310:2319	arg1	conditions					2321:2330	The separation conditions	2306:2330	The separation conditions for these monosaccharides	2306:2356	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	7	130	theme	response	1144:1151	arg1	methodology					1161:1171	response surface methodology	1144:1171	response surface methodology	1144:1171	In this study, two kinds of Pueraria polysaccharides were extracted by an ultrasonic-assisted method, and response surface methodology was performed to explore the conditions for ultrasonic-assisted extraction of polysaccharides from Pueraria.					
34213104	18	131	theme	thomsonii	3794:3802	arg1	Benth					3804:3808	Pueraria thomsonii Benth	3785:3808	Pueraria thomsonii Benth	3785:3808	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	14	132	theme	extraction	2862:2871	arg1	rate					2873:2876	the extraction rate	2858:2876	the extraction rate of polysaccharides from the two kinds of Pueraria	2858:2926	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	19	133	theme	Ohwi	3952:3955	arg1	polysaccharides					3917:3931	The polysaccharides	3913:3931	The polysaccharides of Pueraria lobata Ohwi	3913:3955	The polysaccharides of Pueraria lobata Ohwi were mainly composed of glucose and mannose in the molar ratio 1.00:0.70.					
34213104	0	134	from	analysis	32:39	arg1	polysaccharides					72:86	Pueraria polysaccharides	63:86	Pueraria polysaccharides	63:86	[High-efficiency separation and analysis of monosaccharides in Pueraria polysaccharides by pressurized capillary electrochromatography].					
34213104	18	135	theme	identification	3704:3717	arg1	results					3719:3725	The separation and identification results	3685:3725	The separation and identification results for the actual samples	3685:3748	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	0	136	theme	capillary	103:111	arg1	electrochromatography					113:133	pressurized capillary electrochromatography	91:133	pressurized capillary electrochromatography	91:133	[High-efficiency separation and analysis of monosaccharides in Pueraria polysaccharides by pressurized capillary electrochromatography].					
34213104	1	137	theme	biological	190:199	arg1	bacteriostasis					220:233	bacteriostasis	220:233	bacteriostasis	220:233	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	1	137	theme	biological	190:199	arg1	activities					286:295	immunity boosting activities	268:295	immunity boosting activities	268:295	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	1	137	theme	biological	190:199	arg1	activities					201:210	biological activities	190:210	biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities	190:295	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	9	138	theme	multiple	1915:1922	arg1	equation					1945:1952	the multiple quadratic regression equation	1911:1952	the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides	1911:2023	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	0	139	from	separation	17:26	arg1	polysaccharides					72:86	Pueraria polysaccharides	63:86	Pueraria polysaccharides	63:86	[High-efficiency separation and analysis of monosaccharides in Pueraria polysaccharides by pressurized capillary electrochromatography].					
34213104	14	140	theme	experimental	2772:2783	arg1	factors					2785:2791	the four experimental factors	2763:2791	the four experimental factors	2763:2791	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	7	141	theme	ultrasonic-assisted	1112:1130	arg1	method					1132:1137	an ultrasonic-assisted method	1109:1137	an ultrasonic-assisted method	1109:1137	In this study, two kinds of Pueraria polysaccharides were extracted by an ultrasonic-assisted method, and response surface methodology was performed to explore the conditions for ultrasonic-assisted extraction of polysaccharides from Pueraria.					
34213104	13	142	theme	samples	2695:2701	arg1	kinds					2655:2659	two kinds	2651:2659	two kinds of actual Pueraria polysaccharide samples	2651:2701	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	2	143	from	amount	347:352	arg1	polysaccharides					382:396	these polysaccharides	376:396	these polysaccharides	376:396	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	14	144	theme	greatest	2836:2843	arg1	influence					2845:2853	the greatest influence	2832:2853	the greatest influence	2832:2853	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	9	145	theme	regression	1934:1943	arg1	equation					1945:1952	the multiple quadratic regression equation	1911:1952	the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides	1911:2023	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	18	146	from	mannose	3843:3849	arg1	ratio					3886:3890	the molar ratio 1.00:0.16:0.14:0.07	3876:3910	the molar ratio 1.00:0.16:0.14:0.07	3876:3910	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	12	147	theme	column	2455:2460	arg1	pH					2410:2411	buffer pH	2403:2411	buffer pH	2403:2411	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	12	147	theme	column	2455:2460	arg1	voltage					2422:2428	applied voltage	2414:2428	applied voltage	2414:2428	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	12	147	theme	column	2455:2460	arg1	concentration					2388:2400	the buffer concentration	2377:2400	the buffer concentration	2377:2400	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	12	147	theme	column	2455:2460	arg1	type					2431:2434	type	2431:2434	type of chromatographic column	2431:2460	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	12	147	theme	column	2455:2460	arg1	ratio					2480:2484	mobile phase ratio	2467:2484	mobile phase ratio	2467:2484	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	2	148	theme	monosaccharides	357:371	arg1	monosaccharides					357:371	monosaccharides	357:371	monosaccharides	357:371	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	2	148	theme	monosaccharides	357:371	arg1	composition					330:340	composition	330:340	composition	330:340	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	2	148	theme	monosaccharides	357:371	arg1	amount					347:352	amount	347:352	amount	347:352	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	2	148	theme	monosaccharides	357:371	arg1	structure					319:327	structure	319:327	structure	319:327	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	5	149	theme	saccharide	810:819	arg1	detection					821:829	saccharide detection	810:829	saccharide detection	810:829	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	20	150	theme	monosaccharide	4191:4204	arg1	composition					4206:4216	the monosaccharide composition	4187:4216	the monosaccharide composition of Pueraria polysaccharides	4187:4244	This study provides a novel method for the rapid and efficient separation and detection of neutral monosaccharides, and serves as a reference for analyzing the monosaccharide composition of Pueraria polysaccharides.					
34213104	16	151	theme	separation	3416:3425	arg1	speeds					3441:3446	The separation and detection speeds	3412:3446	The separation and detection speeds	3412:3446	The separation and detection speeds and the column efficiency achieved with this method were much better than those obtained with the traditional liquid chromatography method.					
34213104	16	151	theme	separation	3416:3425	arg1	better					3510:3515	better	3510:3515	better	3510:3515	The separation and detection speeds and the column efficiency achieved with this method were much better than those obtained with the traditional liquid chromatography method.					
34213104	14	152	theme	power	3028:3032	arg1	weak					3049:3052	weak	3049:3052	weak	3049:3052	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	14	152	theme	power	3028:3032	arg1	influence					2969:2977	the influence	2965:2977	the influence of the ultrasonic extraction time and ultrasonic power	2965:3032	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	18	153	theme	separation	3689:3698	arg1	results					3719:3725	The separation and identification results	3685:3725	The separation and identification results for the actual samples	3685:3748	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	16	154	theme	liquid	3558:3563	arg1	method					3580:3585	the traditional liquid chromatography method	3542:3585	the traditional liquid chromatography method	3542:3585	The separation and detection speeds and the column efficiency achieved with this method were much better than those obtained with the traditional liquid chromatography method.					
34213104	5	155	from	present	771:777	arg1	methods					798:804	the commonly used methods	780:804	the commonly used methods for saccharide detection	780:829	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	5	155	from	present	771:777	arg1	spectrometry					931:942	mass spectrometry	926:942	mass spectrometry	926:942	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	5	155	from	present	771:777	arg1	detection					911:919	gas chromatography-ultraviolet detection	880:919	gas chromatography-ultraviolet detection	880:919	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	5	155	from	present	771:777	arg1	detection					869:877	liquid chromatography-ultraviolet detection	835:877	liquid chromatography-ultraviolet detection	835:877	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	3	156	theme	polysaccharides	500:514	arg1	determination					520:532	determination	520:532	determination of the monosaccharide composition	520:566	Therefore, extraction of Pueraria polysaccharides and determination of the monosaccharide composition are of great significance for activity analysis and quality control.					
34213104	3	156	theme	polysaccharides	500:514	arg1	extraction					477:486	extraction	477:486	extraction of Pueraria polysaccharides	477:514	Therefore, extraction of Pueraria polysaccharides and determination of the monosaccharide composition are of great significance for activity analysis and quality control.					
34213104	16	157	theme	detection	3431:3439	arg1	speeds					3441:3446	The separation and detection speeds	3412:3446	The separation and detection speeds	3412:3446	The separation and detection speeds and the column efficiency achieved with this method were much better than those obtained with the traditional liquid chromatography method.					
34213104	16	157	theme	detection	3431:3439	arg1	better					3510:3515	better	3510:3515	better	3510:3515	The separation and detection speeds and the column efficiency achieved with this method were much better than those obtained with the traditional liquid chromatography method.					
34213104	15	158	theme	ammonium	3278:3285	arg1	acetate					3287:3293	acetonitrile-50 mmol/L ammonium acetate	3255:3293	acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1)	3255:3331	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
34213104	4	159	theme	Direct	637:642	arg1	detection					644:652	Direct detection	637:652	Direct detection of saccharides	637:667	Direct detection of saccharides is difficult because they are strongly polar and do not show absorption in the ultraviolet region.					
34213104	2	160	theme	spatial	420:426	arg1	structures					428:437	different spatial structures	410:437	different spatial structures	410:437	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	5	161	theme	mass	926:929	arg1	spectrometry					931:942	mass spectrometry	926:942	mass spectrometry	926:942	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	12	162	theme	buffer	2403:2408	arg1	pH					2410:2411	buffer pH	2403:2411	buffer pH	2403:2411	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	7	163	theme	Pueraria	1066:1073	arg1	polysaccharides					1075:1089	Pueraria polysaccharides	1066:1089	Pueraria polysaccharides	1066:1089	In this study, two kinds of Pueraria polysaccharides were extracted by an ultrasonic-assisted method, and response surface methodology was performed to explore the conditions for ultrasonic-assisted extraction of polysaccharides from Pueraria.					
34213104	13	164	theme	Pueraria	2671:2678	arg1	samples					2695:2701	actual Pueraria polysaccharide samples	2664:2701	actual Pueraria polysaccharide samples	2664:2701	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	14	165	theme	extraction	2997:3006	arg1	time					3008:3011	the ultrasonic extraction time	2982:3011	the ultrasonic extraction time	2982:3011	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	7	166	from	Pueraria	1272:1279	arg1	polysaccharides					1251:1265	polysaccharides	1251:1265	polysaccharides from Pueraria	1251:1279	In this study, two kinds of Pueraria polysaccharides were extracted by an ultrasonic-assisted method, and response surface methodology was performed to explore the conditions for ultrasonic-assisted extraction of polysaccharides from Pueraria.					
34213104	7	166	from	Pueraria	1272:1279	arg1	extraction					1237:1246	ultrasonic-assisted extraction	1217:1246	ultrasonic-assisted extraction of polysaccharides from Pueraria	1217:1279	In this study, two kinds of Pueraria polysaccharides were extracted by an ultrasonic-assisted method, and response surface methodology was performed to explore the conditions for ultrasonic-assisted extraction of polysaccharides from Pueraria.					
34213104	0	167	theme	monosaccharides	44:58	arg1	analysis					32:39	analysis	32:39	analysis	32:39	[High-efficiency separation and analysis of monosaccharides in Pueraria polysaccharides by pressurized capillary electrochromatography].					
34213104	0	167	theme	monosaccharides	44:58	arg1	separation					17:26	[High-efficiency separation	0:26	[High-efficiency separation	0:26	[High-efficiency separation and analysis of monosaccharides in Pueraria polysaccharides by pressurized capillary electrochromatography].					
34213104	6	168	theme	high-efficiency	994:1008	arg1	technology					1026:1035	a high-efficiency microseparation technology	992:1035	a high-efficiency microseparation technology	992:1035	Pressurized capillary electrochromatography is a high-efficiency microseparation technology.					
34213104	6	168	theme	high-efficiency	994:1008	arg1	electrochromatography					967:987	Pressurized capillary electrochromatography	945:987	Pressurized capillary electrochromatography	945:987	Pressurized capillary electrochromatography is a high-efficiency microseparation technology.					
34213104	9	169	theme	liquid-solid	1775:1786	arg1	ratio					1788:1792	liquid-solid ratio	1775:1792	liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g	1775:1825	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	2	170	from	variation	302:310	arg1	monosaccharides					357:371	monosaccharides	357:371	monosaccharides	357:371	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	2	170	from	variation	302:310	arg1	composition					330:340	composition	330:340	composition	330:340	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	2	170	from	variation	302:310	arg1	amount					347:352	amount	347:352	amount	347:352	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	2	170	from	variation	302:310	arg1	structure					319:327	structure	319:327	structure	319:327	The variation in the structure, composition, and amount of monosaccharides in these polysaccharides may lead to different spatial structures and biological activities.					
34213104	9	171	theme	extraction	1981:1990	arg1	rate					1992:1995	the extraction rate	1977:1995	the extraction rate of Pueraria polysaccharides	1977:2023	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	20	172	theme	monosaccharides	4130:4144	arg1	detection					4109:4117	detection	4109:4117	detection	4109:4117	This study provides a novel method for the rapid and efficient separation and detection of neutral monosaccharides, and serves as a reference for analyzing the monosaccharide composition of Pueraria polysaccharides.					
34213104	20	172	theme	monosaccharides	4130:4144	arg1	separation					4094:4103	rapid and efficient separation	4074:4103	rapid and efficient separation	4074:4103	This study provides a novel method for the rapid and efficient separation and detection of neutral monosaccharides, and serves as a reference for analyzing the monosaccharide composition of Pueraria polysaccharides.					
34213104	17	173	theme	good	3665:3668	arg1	repeatability					3670:3682	good repeatability	3665:3682	good repeatability	3665:3682	The results show that the proposed method has a good linear relationship and good repeatability.					
34213104	1	174	theme	immunity	268:275	arg1	activities					286:295	immunity boosting activities	268:295	immunity boosting activities	268:295	Pueraria polysaccharides have been proven to possess biological activities such as bacteriostasis, anti-oxidative, anti-tumor, and immunity boosting activities.					
34213104	0	175	theme	Pueraria	63:70	arg1	polysaccharides					72:86	Pueraria polysaccharides	63:86	Pueraria polysaccharides	63:86	[High-efficiency separation and analysis of monosaccharides in Pueraria polysaccharides by pressurized capillary electrochromatography].					
34213104	8	176	theme	interaction	1286:1296	arg1	effects					1298:1304	The interaction effects	1282:1304	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides	1282:1476	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	14	177	theme	ultrasonic	2794:2803	arg1	temperature					2816:2826	ultrasonic extraction temperature	2794:2826	ultrasonic extraction temperature	2794:2826	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	18	178	theme	Benth	3804:3808	arg1	polysaccharides					3766:3780	the polysaccharides	3762:3780	the polysaccharides of Pueraria thomsonii Benth	3762:3808	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	5	179	theme	used	793:796	arg1	methods					798:804	the commonly used methods	780:804	the commonly used methods for saccharide detection	780:829	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	5	179	theme	used	793:796	arg1	detection					869:877	liquid chromatography-ultraviolet detection	835:877	liquid chromatography-ultraviolet detection	835:877	At present, the commonly used methods for saccharide detection are liquid chromatography-ultraviolet detection, gas chromatography-ultraviolet detection, and mass spectrometry.					
34213104	12	180	theme	applied	2414:2420	arg1	voltage					2422:2428	applied voltage	2414:2428	applied voltage	2414:2428	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	18	181	from	glucose	3834:3840	arg1	ratio					3886:3890	the molar ratio 1.00:0.16:0.14:0.07	3876:3910	the molar ratio 1.00:0.16:0.14:0.07	3876:3910	The separation and identification results for the actual samples showed that the polysaccharides of Pueraria thomsonii Benth were mainly composed of glucose, mannose, rhamnose, and fucose in the molar ratio 1.00:0.16:0.14:0.07.					
34213104	13	182	theme	established	2515:2525	arg1	method					2593:2598	the established pressurized capillary electrochromatography-ultraviolet detection method	2511:2598	the established pressurized capillary electrochromatography-ultraviolet detection method	2511:2598	Finally, the established pressurized capillary electrochromatography-ultraviolet detection method was applied to the detection and identification of two kinds of actual Pueraria polysaccharide samples.					
34213104	6	183	theme	capillary	957:965	arg1	technology					1026:1035	a high-efficiency microseparation technology	992:1035	a high-efficiency microseparation technology	992:1035	Pressurized capillary electrochromatography is a high-efficiency microseparation technology.					
34213104	6	183	theme	capillary	957:965	arg1	electrochromatography					967:987	Pressurized capillary electrochromatography	945:987	Pressurized capillary electrochromatography	945:987	Pressurized capillary electrochromatography is a high-efficiency microseparation technology.					
34213104	9	184	from	equation	1945:1952	arg1	rate					1992:1995	the extraction rate	1977:1995	the extraction rate of Pueraria polysaccharides	1977:2023	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	185	theme	polysaccharides	2009:2023	arg1	rate					1992:1995	the extraction rate	1977:1995	the extraction rate of Pueraria polysaccharides	1977:2023	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	12	186	theme	buffer	2381:2386	arg1	concentration					2388:2400	the buffer concentration	2377:2400	the buffer concentration	2377:2400	The separation conditions for these monosaccharides were explored, and the buffer concentration, buffer pH, applied voltage, type of chromatographic column, and mobile phase ratio were optimized.					
34213104	3	187	theme	composition	556:566	arg1	determination					520:532	determination	520:532	determination of the monosaccharide composition	520:566	Therefore, extraction of Pueraria polysaccharides and determination of the monosaccharide composition are of great significance for activity analysis and quality control.					
34213104	3	187	theme	composition	556:566	arg1	extraction					477:486	extraction	477:486	extraction of Pueraria polysaccharides	477:514	Therefore, extraction of Pueraria polysaccharides and determination of the monosaccharide composition are of great significance for activity analysis and quality control.					
34213104	9	188	theme	equipment	1570:1578	arg1	conditions					1580:1589	the actual equipment conditions	1559:1589	the actual equipment conditions	1559:1589	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	188	theme	equipment	1570:1578	arg1	W.					1886:1887	W.	1886:1887	W.	1886:1887	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	9	188	theme	equipment	1570:1578	arg1	conditions					1615:1624	the optimal extraction conditions	1592:1624	the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power	1592:1879	By combining the optimal conditions predicted by the software and the actual equipment conditions, the optimal extraction conditions for Pueraria polysaccharides were chosen as follows:ultrasonic extraction temperature, 90℃; liquid-solid ratio of Pueraria thomsonii Benth, 20 mL/g; liquid-solid ratio of Pueraria lobata Ohwi, 40 mL/g; ultrasonic extraction time, 30 min; ultrasonic power, 180 W. Through data fitting, the multiple quadratic regression equation of the four factors on the extraction rate of Pueraria polysaccharides was established.					
34213104	8	189	theme	factors	1314:1320	arg1	effects					1298:1304	The interaction effects	1282:1304	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides	1282:1476	The interaction effects of four factors, the liquid-solid ratio, ultrasonic extraction time, ultrasonic extraction temperature, and ultrasonic power, on the extraction rate of the polysaccharides were analyzed.					
34213104	14	190	theme	liquid-solid	2945:2956	arg1	ratio					2958:2962	the liquid-solid ratio	2941:2962	the liquid-solid ratio	2941:2962	The results of response surface analysis showed that among the four experimental factors, ultrasonic extraction temperature had the greatest influence on the extraction rate of polysaccharides from the two kinds of Pueraria, followed by the liquid-solid ratio; the influence of the ultrasonic extraction time and ultrasonic power was relatively weak.					
34213104	20	191	theme	polysaccharides	4230:4244	arg1	composition					4206:4216	the monosaccharide composition	4187:4216	the monosaccharide composition of Pueraria polysaccharides	4187:4244	This study provides a novel method for the rapid and efficient separation and detection of neutral monosaccharides, and serves as a reference for analyzing the monosaccharide composition of Pueraria polysaccharides.					
34213104	10	192	theme	capillary	2078:2086	arg1	electrochromatography					2088:2108	pressurized capillary electrochromatography	2066:2108	pressurized capillary electrochromatography for the separation and analysis of eight neutral monosaccharides	2066:2173	A novel method based on pressurized capillary electrochromatography for the separation and analysis of eight neutral monosaccharides has been established.					
34213104	3	193	theme	activity	598:605	arg1	analysis					607:614	activity analysis	598:614	activity analysis	598:614	Therefore, extraction of Pueraria polysaccharides and determination of the monosaccharide composition are of great significance for activity analysis and quality control.					
34213104	15	194	theme	aqueous	3295:3301	arg1	solution					3303:3310	acetonitrile-50 mmol/L ammonium acetate aqueous solution	3255:3310	acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1)	3255:3331	The experimental conditions were determined as follows:the separation of eight neutral monosaccharide derivatives could be realized within 24 min on a Halo-2.7 μm core-shell C18 capillary column with acetonitrile-50 mmol/L ammonium acetate aqueous solution (18:82, v/v, pH 4.1) as the mobile phase, by detection at 250 nm under an applied voltage of-20 kV.					
32846866	6	0	theme	liquid-solid	1211:1222	arg1	conditions					1135:1144	the optimal extraction conditions	1112:1144	the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g)	1112:1239	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	0	theme	liquid-solid	1211:1222	arg1	mL/g					1235:1238	liquid-solid ratio 1.66 mL/g	1211:1238	liquid-solid ratio 1.66 mL/g	1211:1238	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	1	theme	desirability	1248:1259	arg1	value					1261:1265	a desirability value	1246:1265	a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments	1246:1377	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	9	2	theme	radical	1665:1671	arg1	damage					1673:1678	free radical damage	1660:1678	free radical damage	1660:1678	The results showed that the RAP extract exhibited a strong potential against free radical damage.					
32846866	2	3	theme	solid-liquid	525:536	arg1	ratio					538:542	solid-liquid ratio	525:542	solid-liquid ratio (1-2 g/mL)	525:553	Three parameters that affect the productivity of UAE, such as extraction temperature (50-90 °C), extraction time (10-30 min), and solid-liquid ratio (1-2 g/mL), were examined to optimize the yield of the polysaccharide percentage.					
32846866	2	3	theme	solid-liquid	525:536	arg1	g/mL					549:552	1-2 g/mL	545:552	1-2 g/mL	545:552	Three parameters that affect the productivity of UAE, such as extraction temperature (50-90 °C), extraction time (10-30 min), and solid-liquid ratio (1-2 g/mL), were examined to optimize the yield of the polysaccharide percentage.					
32846866	7	4	theme	polysaccharide	1411:1424	arg1	yield					1426:1430	polysaccharide yield	1411:1430	polysaccharide yield	1411:1430	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	7	5	theme	optimized	1448:1456	arg1	conditions					1469:1478	optimized extraction conditions	1448:1478	optimized extraction conditions with a 95.43% validity	1448:1501	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	1	6	theme	RAP	281:283	arg1	yield					286:290	Rhododendron arboreum polysaccharide (RAP) yield	243:290	Rhododendron arboreum polysaccharide (RAP) yield	243:290	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	8	7	dep	in	1508:1509	arg1	vitro					1511:1515	vitro	1511:1515	vitro	1511:1515	The in vitro antioxidant effect of polysaccharides of R. arboreum was studied.					
32846866	7	8	theme	±	1400:1400	arg1	%					1406:1406	11.09 ± 1.65%	1394:1406	11.09 ± 1.65% of polysaccharide yield	1394:1430	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	7	8	theme	±	1400:1400	arg1	yield					1426:1430	polysaccharide yield	1411:1430	polysaccharide yield	1411:1430	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	4	9	theme	regression	842:851	arg1	analysis					853:860	multiple regression analysis	833:860	multiple regression analysis	833:860	The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999).					
32846866	2	10	theme	percentage	614:623	arg1	yield					586:590	the yield	582:590	the yield of the polysaccharide percentage	582:623	Three parameters that affect the productivity of UAE, such as extraction temperature (50-90 °C), extraction time (10-30 min), and solid-liquid ratio (1-2 g/mL), were examined to optimize the yield of the polysaccharide percentage.					
32846866	6	11	theme	66.75	1170:1174	arg1	°C					1176:1177	extraction temperature 66.75 °C	1147:1177	extraction temperature 66.75 °C	1147:1177	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	11	theme	66.75	1170:1174	arg1	conditions					1135:1144	the optimal extraction conditions	1112:1144	the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g)	1112:1239	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	7	12	theme	%	1406:1406	arg1	average					1383:1389	An average	1380:1389	An average of 11.09 ± 1.65% of polysaccharide yield	1380:1430	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	4	13	theme	multiple	833:840	arg1	analysis					853:860	multiple regression analysis	833:860	multiple regression analysis	833:860	The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999).					
32846866	2	14	theme	polysaccharide	599:612	arg1	percentage					614:623	the polysaccharide percentage	595:623	the polysaccharide percentage	595:623	Three parameters that affect the productivity of UAE, such as extraction temperature (50-90 °C), extraction time (10-30 min), and solid-liquid ratio (1-2 g/mL), were examined to optimize the yield of the polysaccharide percentage.					
32846866	6	15	theme	temperature	1158:1168	arg1	°C					1176:1177	extraction temperature 66.75 °C	1147:1177	extraction temperature 66.75 °C	1147:1177	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	15	theme	temperature	1158:1168	arg1	conditions					1135:1144	the optimal extraction conditions	1112:1144	the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g)	1112:1239	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	16	theme	highest	1284:1290	arg1	%					1324:1324	11.56%	1319:1324	11.56%	1319:1324	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	16	theme	highest	1284:1290	arg1	percentage					1307:1316	polysaccharide percentage	1292:1316	polysaccharide percentage (11.56%)	1292:1325	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	1	17	theme	yield	286:290	arg1	conditions					212:221	the ultrasound-assisted extraction (UAE) conditions	171:221	the ultrasound-assisted extraction (UAE) conditions	171:221	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	1	17	theme	yield	286:290	arg1	optimization					227:238	optimization	227:238	optimization of Rhododendron arboreum polysaccharide (RAP) yield	227:290	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	0	18	theme	Antioxidant	118:128	arg1	Potential					130:138	Its Antioxidant Potential	114:138	Its Antioxidant Potential	114:138	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	1	19	theme	antioxidant	372:382	arg1	potential					384:392	its antioxidant potential	368:392	its antioxidant potential	368:392	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	4	20	theme	polynomial	796:805	arg1	models					816:821	polynomial response models	796:821	polynomial response models	796:821	The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999).					
32846866	6	21	theme	extraction	1147:1156	arg1	°C					1176:1177	extraction temperature 66.75 °C	1147:1177	extraction temperature 66.75 °C	1147:1177	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	21	theme	extraction	1147:1156	arg1	conditions					1135:1144	the optimal extraction conditions	1112:1144	the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g)	1112:1239	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	1	22	theme	ultrasound-assisted	175:193	arg1	conditions					212:221	the ultrasound-assisted extraction (UAE) conditions	171:221	the ultrasound-assisted extraction (UAE) conditions	171:221	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	4	23	theme	=	913:913	arg1	R2					910:911	R2	910:911	R2 = 0.999	910:919	The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999).					
32846866	1	24	theme	potential	384:392	arg1	evaluation					354:363	the evaluation	350:363	the evaluation of its antioxidant potential	350:392	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	1	24	theme	potential	384:392	arg1	design					339:344	a Box-Behnken response surface design	308:344	a Box-Behnken response surface design	308:344	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	8	25	theme	polysaccharides	1539:1553	arg1	effect					1529:1534	The in vitro antioxidant effect	1504:1534	The in vitro antioxidant effect of polysaccharides of R. arboreum	1504:1568	The in vitro antioxidant effect of polysaccharides of R. arboreum was studied.					
32846866	1	26	theme	extraction	195:204	arg1	conditions					212:221	the ultrasound-assisted extraction (UAE) conditions	171:221	the ultrasound-assisted extraction (UAE) conditions	171:221	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	0	27	theme	Response	12:19	arg1	Design					29:34	Box-Behnken Response Surface Design	0:34	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum	0:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	8	28	theme	antioxidant	1517:1527	arg1	effect					1529:1534	The in vitro antioxidant effect	1504:1534	The in vitro antioxidant effect of polysaccharides of R. arboreum	1504:1568	The in vitro antioxidant effect of polysaccharides of R. arboreum was studied.					
32846866	6	29	theme	ratio	1224:1228	arg1	conditions					1135:1144	the optimal extraction conditions	1112:1144	the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g)	1112:1239	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	29	theme	ratio	1224:1228	arg1	mL/g					1235:1238	liquid-solid ratio 1.66 mL/g	1211:1238	liquid-solid ratio 1.66 mL/g	1211:1238	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	7	30	with	conditions	1469:1478	arg1	validity					1494:1501	a 95.43% validity	1485:1501	a 95.43% validity	1485:1501	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	6	31	dep	conditions	1135:1144	arg1	conditions					1135:1144	the optimal extraction conditions	1112:1144	the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g)	1112:1239	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	31	dep	conditions	1135:1144	arg1	min					1202:1204	extraction time 19.72 min	1180:1204	extraction time 19.72 min	1180:1204	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	31	dep	conditions	1135:1144	arg1	°C					1176:1177	extraction temperature 66.75 °C	1147:1177	extraction temperature 66.75 °C	1147:1177	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	31	dep	conditions	1135:1144	arg1	mL/g					1235:1238	liquid-solid ratio 1.66 mL/g	1211:1238	liquid-solid ratio 1.66 mL/g	1211:1238	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	0	32	theme	Box-Behnken	0:10	arg1	Design					29:34	Box-Behnken Response Surface Design	0:34	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum	0:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	9	33	theme	free	1660:1663	arg1	damage					1673:1678	free radical damage	1660:1678	free radical damage	1660:1678	The results showed that the RAP extract exhibited a strong potential against free radical damage.					
32846866	3	34	theme	chromatographic	630:644	arg1	analysis					646:653	The chromatographic analysis	626:653	The chromatographic analysis	626:653	The chromatographic analysis revealed that the composition of monosaccharides was found to be glucose, galactose, mannose, arabinose, and fucose.					
32846866	6	35	theme	extraction	1124:1133	arg1	min					1202:1204	extraction time 19.72 min	1180:1204	extraction time 19.72 min	1180:1204	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	35	theme	extraction	1124:1133	arg1	°C					1176:1177	extraction temperature 66.75 °C	1147:1177	extraction temperature 66.75 °C	1147:1177	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	35	theme	extraction	1124:1133	arg1	conditions					1135:1144	the optimal extraction conditions	1112:1144	the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g)	1112:1239	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	35	theme	extraction	1124:1133	arg1	mL/g					1235:1238	liquid-solid ratio 1.66 mL/g	1211:1238	liquid-solid ratio 1.66 mL/g	1211:1238	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	36	theme	polysaccharide	1292:1305	arg1	%					1324:1324	11.56%	1319:1324	11.56%	1319:1324	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	36	theme	polysaccharide	1292:1305	arg1	percentage					1307:1316	polysaccharide percentage	1292:1316	polysaccharide percentage (11.56%)	1292:1325	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	1	37	theme	UAE	207:209	arg1	conditions					212:221	the ultrasound-assisted extraction (UAE) conditions	171:221	the ultrasound-assisted extraction (UAE) conditions	171:221	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	8	38	theme	R.	1558:1559	arg1	arboreum					1561:1568	R. arboreum	1558:1568	R. arboreum	1558:1568	The in vitro antioxidant effect of polysaccharides of R. arboreum was studied.					
32846866	9	39	theme	strong	1635:1640	arg1	potential					1642:1650	a strong potential	1633:1650	a strong potential against free radical damage	1633:1678	The results showed that the RAP extract exhibited a strong potential against free radical damage.					
32846866	6	40	theme	optimal	1116:1122	arg1	min					1202:1204	extraction time 19.72 min	1180:1204	extraction time 19.72 min	1180:1204	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	40	theme	optimal	1116:1122	arg1	°C					1176:1177	extraction temperature 66.75 °C	1147:1177	extraction temperature 66.75 °C	1147:1177	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	40	theme	optimal	1116:1122	arg1	conditions					1135:1144	the optimal extraction conditions	1112:1144	the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g)	1112:1239	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	40	theme	optimal	1116:1122	arg1	mL/g					1235:1238	liquid-solid ratio 1.66 mL/g	1211:1238	liquid-solid ratio 1.66 mL/g	1211:1238	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	2	41	theme	UAE	444:446	arg1	productivity					428:439	the productivity	424:439	the productivity of UAE	424:446	Three parameters that affect the productivity of UAE, such as extraction temperature (50-90 °C), extraction time (10-30 min), and solid-liquid ratio (1-2 g/mL), were examined to optimize the yield of the polysaccharide percentage.					
32846866	0	42	theme	Surface	21:27	arg1	Design					29:34	Box-Behnken Response Surface Design	0:34	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum	0:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	8	43	theme	in	1508:1509	arg1	effect					1529:1534	The in vitro antioxidant effect	1504:1534	The in vitro antioxidant effect of polysaccharides of R. arboreum	1504:1568	The in vitro antioxidant effect of polysaccharides of R. arboreum was studied.					
32846866	0	44	theme	Potential	130:138	arg1	Design					29:34	Box-Behnken Response Surface Design	0:34	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum	0:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	0	44	theme	Potential	130:138	arg1	Evaluation					100:109	the Evaluation	96:109	the Evaluation of Its Antioxidant Potential	96:138	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	7	45	theme	95.43	1487:1491	arg1	%					1492:1492	%	1492:1492	%	1492:1492	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	6	46	theme	prediction	1077:1086	arg1	tool					1088:1091	Derringer's desirability prediction tool	1052:1091	Derringer's desirability prediction tool	1052:1091	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	0	47	theme	Polysaccharide	39:52	arg1	Extraction					54:63	Polysaccharide Extraction	39:63	Polysaccharide Extraction from Rhododendron arboreum	39:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	4	48	theme	value	903:907	arg1	coefficient					874:884	a high coefficient	867:884	a high coefficient of determination value (R2 = 0.999)	867:920	The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999).					
32846866	5	49	theme	polysaccharide	1025:1038	arg1	percentage					1040:1049	polysaccharide percentage	1025:1049	polysaccharide percentage	1025:1049	The data exhibited that the extraction parameters have significant effects on the extraction yield of polysaccharide percentage.					
32846866	1	50	theme	Box-Behnken	310:320	arg1	design					339:344	a Box-Behnken response surface design	308:344	a Box-Behnken response surface design	308:344	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	4	51	theme	determination	889:901	arg1	value					903:907	determination value	889:907	determination value	889:907	The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999).					
32846866	0	52	from	arboreum	83:90	arg1	Design					29:34	Box-Behnken Response Surface Design	0:34	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum	0:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	0	52	from	arboreum	83:90	arg1	Extraction					54:63	Polysaccharide Extraction	39:63	Polysaccharide Extraction from Rhododendron arboreum	39:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	0	52	from	arboreum	83:90	arg1	Evaluation					100:109	the Evaluation	96:109	the Evaluation of Its Antioxidant Potential	96:138	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	8	53	theme	arboreum	1561:1568	arg1	polysaccharides					1539:1553	polysaccharides	1539:1553	polysaccharides of R. arboreum	1539:1568	The in vitro antioxidant effect of polysaccharides of R. arboreum was studied.					
32846866	4	54	dep	coefficient	874:884	arg1	R2					910:911	R2	910:911	R2 = 0.999	910:919	The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999).					
32846866	6	55	theme	validation	1356:1365	arg1	experiments					1367:1377	validation experiments	1356:1377	validation experiments	1356:1377	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	1	56	theme	response	322:329	arg1	design					339:344	a Box-Behnken response surface design	308:344	a Box-Behnken response surface design	308:344	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	4	57	dep	=	913:913	arg1	0.999					915:919	0.999	915:919	0.999	915:919	The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999).					
32846866	2	58	theme	extraction	492:501	arg1	time					503:506	extraction time	492:506	extraction time (10-30 min)	492:518	Three parameters that affect the productivity of UAE, such as extraction temperature (50-90 °C), extraction time (10-30 min), and solid-liquid ratio (1-2 g/mL), were examined to optimize the yield of the polysaccharide percentage.					
32846866	2	58	theme	extraction	492:501	arg1	min					515:517	10-30 min	509:517	10-30 min	509:517	Three parameters that affect the productivity of UAE, such as extraction temperature (50-90 °C), extraction time (10-30 min), and solid-liquid ratio (1-2 g/mL), were examined to optimize the yield of the polysaccharide percentage.					
32846866	5	59	theme	significant	978:988	arg1	effects					990:996	significant effects	978:996	significant effects	978:996	The data exhibited that the extraction parameters have significant effects on the extraction yield of polysaccharide percentage.					
32846866	3	60	theme	monosaccharides	688:702	arg1	composition					673:683	the composition	669:683	the composition of monosaccharides	669:702	The chromatographic analysis revealed that the composition of monosaccharides was found to be glucose, galactose, mannose, arabinose, and fucose.					
32846866	3	60	theme	monosaccharides	688:702	arg1	glucose					720:726	glucose	720:726	glucose	720:726	The chromatographic analysis revealed that the composition of monosaccharides was found to be glucose, galactose, mannose, arabinose, and fucose.					
32846866	0	61	theme	Extraction	54:63	arg1	Design					29:34	Box-Behnken Response Surface Design	0:34	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum	0:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	0	61	theme	Extraction	54:63	arg1	Evaluation					100:109	the Evaluation	96:109	the Evaluation of Its Antioxidant Potential	96:138	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	1	62	theme	surface	331:337	arg1	design					339:344	a Box-Behnken response surface design	308:344	a Box-Behnken response surface design	308:344	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	6	63	theme	1	1270:1270	arg1	value					1261:1265	a desirability value	1246:1265	a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments	1246:1377	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	7	64	theme	extraction	1458:1467	arg1	conditions					1469:1478	optimized extraction conditions	1448:1478	optimized extraction conditions with a 95.43% validity	1448:1501	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	5	65	theme	percentage	1040:1049	arg1	yield					1016:1020	the extraction yield	1001:1020	the extraction yield of polysaccharide percentage	1001:1049	The data exhibited that the extraction parameters have significant effects on the extraction yield of polysaccharide percentage.					
32846866	7	66	theme	%	1492:1492	arg1	validity					1494:1501	a 95.43% validity	1485:1501	a 95.43% validity	1485:1501	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	4	67	theme	high	869:872	arg1	coefficient					874:884	a high coefficient	867:884	a high coefficient of determination value (R2 = 0.999)	867:920	The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999).					
32846866	6	68	theme	time	1191:1194	arg1	min					1202:1204	extraction time 19.72 min	1180:1204	extraction time 19.72 min	1180:1204	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	68	theme	time	1191:1194	arg1	conditions					1135:1144	the optimal extraction conditions	1112:1144	the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g)	1112:1239	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	7	69	theme	yield	1426:1430	arg1	%					1406:1406	11.09 ± 1.65%	1394:1406	11.09 ± 1.65% of polysaccharide yield	1394:1430	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	7	69	theme	yield	1426:1430	arg1	yield					1426:1430	polysaccharide yield	1411:1430	polysaccharide yield	1411:1430	An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity.					
32846866	9	70	theme	RAP	1611:1613	arg1	extract					1615:1621	the RAP extract	1607:1621	the RAP extract	1607:1621	The results showed that the RAP extract exhibited a strong potential against free radical damage.					
32846866	5	71	theme	extraction	1005:1014	arg1	yield					1016:1020	the extraction yield	1001:1020	the extraction yield of polysaccharide percentage	1001:1049	The data exhibited that the extraction parameters have significant effects on the extraction yield of polysaccharide percentage.					
32846866	6	72	theme	extraction	1180:1189	arg1	min					1202:1204	extraction time 19.72 min	1180:1204	extraction time 19.72 min	1180:1204	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	72	theme	extraction	1180:1189	arg1	conditions					1135:1144	the optimal extraction conditions	1112:1144	the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g)	1112:1239	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	6	73	theme	desirability	1064:1075	arg1	tool					1088:1091	Derringer's desirability prediction tool	1052:1091	Derringer's desirability prediction tool	1052:1091	Derringer's desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 °C, extraction time 19.72 min, and liquid-solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments.					
32846866	5	74	contain	have	973:976	arg2	effects					990:996	significant effects	978:996	significant effects	978:996	The data exhibited that the extraction parameters have significant effects on the extraction yield of polysaccharide percentage.					
32846866	5	74	contain	have	973:976	arg1	parameters					962:971	the extraction parameters	947:971	the extraction parameters	947:971	The data exhibited that the extraction parameters have significant effects on the extraction yield of polysaccharide percentage.					
32846866	1	75	theme	Rhododendron	243:254	arg1	yield					286:290	Rhododendron arboreum polysaccharide (RAP) yield	243:290	Rhododendron arboreum polysaccharide (RAP) yield	243:290	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	0	76	from	Evaluation	100:109	arg1	arboreum					83:90	Rhododendron arboreum	70:90	Rhododendron arboreum	70:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	1	77	theme	present	148:154	arg1	investigation					156:168	the present investigation	144:168	the present investigation	144:168	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	1	78	theme	arboreum	256:263	arg1	yield					286:290	Rhododendron arboreum polysaccharide (RAP) yield	243:290	Rhododendron arboreum polysaccharide (RAP) yield	243:290	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
32846866	0	79	theme	Rhododendron	70:81	arg1	arboreum					83:90	Rhododendron arboreum	70:90	Rhododendron arboreum	70:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	0	80	from	Design	29:34	arg1	arboreum					83:90	Rhododendron arboreum	70:90	Rhododendron arboreum	70:90	Box-Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential.					
32846866	5	81	theme	extraction	951:960	arg1	parameters					962:971	the extraction parameters	947:971	the extraction parameters	947:971	The data exhibited that the extraction parameters have significant effects on the extraction yield of polysaccharide percentage.					
32846866	4	82	theme	response	807:814	arg1	models					816:821	polynomial response models	796:821	polynomial response models	796:821	The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999).					
32846866	2	83	theme	extraction	457:466	arg1	temperature					468:478	extraction temperature	457:478	extraction temperature (50-90 °C)	457:489	Three parameters that affect the productivity of UAE, such as extraction temperature (50-90 °C), extraction time (10-30 min), and solid-liquid ratio (1-2 g/mL), were examined to optimize the yield of the polysaccharide percentage.					
32846866	2	83	theme	extraction	457:466	arg1	°C					487:488	50-90 °C	481:488	50-90 °C	481:488	Three parameters that affect the productivity of UAE, such as extraction temperature (50-90 °C), extraction time (10-30 min), and solid-liquid ratio (1-2 g/mL), were examined to optimize the yield of the polysaccharide percentage.					
32846866	1	84	theme	polysaccharide	265:278	arg1	yield					286:290	Rhododendron arboreum polysaccharide (RAP) yield	243:290	Rhododendron arboreum polysaccharide (RAP) yield	243:290	In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box-Behnken response surface design and the evaluation of its antioxidant potential.					
34062454	2	0	theme	ultrasonic	456:465	arg1	extractions					467:477	ultrasonic extractions	456:477	ultrasonic extractions at different frequencies	456:502	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	3	1	theme	highest	1099:1105	arg1	%					1133:1133	76.08%	1128:1133	76.08%	1128:1133	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	1	theme	highest	1099:1105	arg1	content					1119:1125	the highest carboxylate content	1095:1125	the highest carboxylate content (76.08%)	1095:1134	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	1	2	theme	physicochemical	303:317	arg1	characteristics					319:333	the physicochemical characteristics	299:333	the physicochemical characteristics	299:333	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	1	3	theme	okra	240:243	arg1	pods					282:285	okra (Abelmoschus esculentus (L.) Moench) pods	240:285	okra (Abelmoschus esculentus (L.) Moench) pods (OPSs)	240:292	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	1	3	theme	okra	240:243	arg1	OPSs					288:291	OPSs	288:291	OPSs	288:291	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	3	4	theme	nitrite	991:997	arg1	capacities					1014:1023	nitrite ion absorption capacities	991:1023	nitrite ion absorption capacities	991:1023	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	5	theme	lowest	825:830	arg1	MWs					832:834	the lowest MWs	821:834	the lowest MWs (0.85-14.93 × 105 Da)	821:856	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	5	theme	lowest	825:830	arg1	Da					854:855	0.85-14.93 × 105 Da	837:855	0.85-14.93 × 105 Da	837:855	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	0	6	theme	different	109:117	arg1	frequencies					119:129	different frequencies	109:129	different frequencies	109:129	Physicochemical and functional characteristics of polysaccharides from okra extracted by using ultrasound at different frequencies.					
34062454	2	7	theme	monosaccharide	576:589	arg1	compositions					591:602	monosaccharide compositions	576:602	monosaccharide compositions	576:602	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	1	8	theme	OPSs	401:404	arg1	characteristics					319:333	the physicochemical characteristics	299:333	the physicochemical characteristics	299:333	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	1	8	theme	OPSs	401:404	arg1	properties					347:356	functional properties	336:356	functional properties	336:356	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	1	8	theme	OPSs	401:404	arg1	activities					383:392	in vitro biological activities	363:392	in vitro biological activities	363:392	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	1	9	theme	Abelmoschus	246:256	arg1	Moench					274:279	Abelmoschus esculentus (L.) Moench	246:279	Abelmoschus esculentus (L.) Moench	246:279	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	1	9	theme	Abelmoschus	246:256	arg1	okra					240:243	okra	240:243	okra (Abelmoschus esculentus (L.) Moench) pods (OPSs)	240:292	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	0	10	from	okra	71:74	arg1	polysaccharides					50:64	polysaccharides	50:64	polysaccharides from okra	50:74	Physicochemical and functional characteristics of polysaccharides from okra extracted by using ultrasound at different frequencies.					
34062454	0	10	from	okra	71:74	arg1	characteristics					31:45	Physicochemical and functional characteristics	0:45	Physicochemical and functional characteristics of polysaccharides from okra	0:74	Physicochemical and functional characteristics of polysaccharides from okra extracted by using ultrasound at different frequencies.					
34062454	1	11	from	pods	282:285	arg1	polysaccharides					219:233	extract polysaccharides	211:233	extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs)	211:292	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	2	12	theme	thermal	753:759	arg1	stabilities					761:771	thermal stabilities	753:771	thermal stabilities	753:771	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	1	13	theme	esculentus	258:267	arg1	Moench					274:279	Abelmoschus esculentus (L.) Moench	246:279	Abelmoschus esculentus (L.) Moench	246:279	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	1	13	theme	esculentus	258:267	arg1	okra					240:243	okra	240:243	okra (Abelmoschus esculentus (L.) Moench) pods (OPSs)	240:292	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	3	14	theme	polyphenol	870:879	arg1	content					881:887	highest polyphenol content	862:887	highest polyphenol content (7.38%)	862:895	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	14	theme	polyphenol	870:879	arg1	%					894:894	7.38%	890:894	7.38%	890:894	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	2	15	theme	different	482:490	arg1	frequencies					492:502	different frequencies	482:502	different frequencies	482:502	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	1	16	theme	extract	211:217	arg1	polysaccharides					219:233	extract polysaccharides	211:233	extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs)	211:292	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	1	17	theme	dual-frequency	165:178	arg1	ultrasounds					186:196	single- (SFU) and dual-frequency (DFU) ultrasounds	147:196	single- (SFU) and dual-frequency (DFU) ultrasounds	147:196	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	1	18	theme	functional	336:345	arg1	properties					347:356	functional properties	336:356	functional properties	336:356	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	2	19	theme	rheological	656:666	arg1	properties					668:677	rheological properties	656:677	rheological properties of the OPSs	656:689	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	20	theme	structural	729:738	arg1	features					740:747	their preliminary structural features	711:747	their preliminary structural features	711:747	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	0	21	theme	Physicochemical	0:14	arg1	characteristics					31:45	Physicochemical and functional characteristics	0:45	Physicochemical and functional characteristics of polysaccharides from okra	0:74	Physicochemical and functional characteristics of polysaccharides from okra extracted by using ultrasound at different frequencies.					
34062454	2	22	theme	molecular	605:613	arg1	weights					615:621	molecular weights	605:621	molecular weights (MWs)	605:627	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	22	theme	molecular	605:613	arg1	MWs					624:626	MWs	624:626	MWs	624:626	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	3	23	theme	absorption	1003:1012	arg1	capacities					1014:1023	nitrite ion absorption capacities	991:1023	nitrite ion absorption capacities	991:1023	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	24	with	structures	924:933	arg1	Da					854:855	0.85-14.93 × 105 Da	837:855	0.85-14.93 × 105 Da	837:855	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	24	with	structures	924:933	arg1	content					881:887	highest polyphenol content	862:887	highest polyphenol content (7.38%)	862:895	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	24	with	structures	924:933	arg1	%					894:894	7.38%	890:894	7.38%	890:894	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	24	with	structures	924:933	arg1	MWs					832:834	the lowest MWs	821:834	the lowest MWs (0.85-14.93 × 105 Da)	821:856	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	1	25	theme	L.	270:271	arg1	Moench					274:279	Abelmoschus esculentus (L.) Moench	246:279	Abelmoschus esculentus (L.) Moench	246:279	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	1	25	theme	L.	270:271	arg1	okra					240:243	okra	240:243	okra (Abelmoschus esculentus (L.) Moench) pods (OPSs)	240:292	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	2	26	theme	extraction	536:545	arg1	yields					547:552	extraction yields	536:552	extraction yields	536:552	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	3	27	dep	higher	1210:1215	arg1	capacity					1235:1242	bile acid-binding capacity	1217:1242	bile acid-binding capacity	1217:1242	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	1	28	dep	single-	147:153	arg1	SFU					156:158	SFU	156:158	SFU	156:158	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	0	29	theme	functional	20:29	arg1	characteristics					31:45	Physicochemical and functional characteristics	0:45	Physicochemical and functional characteristics of polysaccharides from okra	0:74	Physicochemical and functional characteristics of polysaccharides from okra extracted by using ultrasound at different frequencies.					
34062454	0	30	theme	polysaccharides	50:64	arg1	characteristics					31:45	Physicochemical and functional characteristics	0:45	Physicochemical and functional characteristics of polysaccharides from okra	0:74	Physicochemical and functional characteristics of polysaccharides from okra extracted by using ultrasound at different frequencies.					
34062454	3	31	with	20 kHz	1083:1088	arg1	%					1133:1133	76.08%	1128:1133	76.08%	1128:1133	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	31	with	20 kHz	1083:1088	arg1	MWs					1137:1139	MWs	1137:1139	MWs (7.28-32.83 × 105 Da)	1137:1161	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	31	with	20 kHz	1083:1088	arg1	Da					1159:1160	7.28-32.83 × 105 Da	1142:1160	7.28-32.83 × 105 Da	1142:1160	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	31	with	20 kHz	1083:1088	arg1	degree					1167:1172	degree	1167:1172	degree of esterification (30.7%)	1167:1198	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	31	with	20 kHz	1083:1088	arg1	content					1119:1125	the highest carboxylate content	1095:1125	the highest carboxylate content (76.08%)	1095:1134	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	32	from	structures	924:933	arg1	DFU					799:801	DFU	799:801	DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures	799:933	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	1	33	used	used	203:206	arg2	ultrasounds					186:196	single- (SFU) and dual-frequency (DFU) ultrasounds	147:196	single- (SFU) and dual-frequency (DFU) ultrasounds	147:196	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	3	34	theme	bile	1217:1220	arg1	capacity					1235:1242	bile acid-binding capacity	1217:1242	bile acid-binding capacity	1217:1242	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	2	35	theme	remarkable	511:520	arg1	variations					522:531	remarkable variations	511:531	remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs	511:689	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	3	36	with	40/60 kHz	806:814	arg1	Da					854:855	0.85-14.93 × 105 Da	837:855	0.85-14.93 × 105 Da	837:855	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	36	with	40/60 kHz	806:814	arg1	content					881:887	highest polyphenol content	862:887	highest polyphenol content (7.38%)	862:895	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	36	with	40/60 kHz	806:814	arg1	%					894:894	7.38%	890:894	7.38%	890:894	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	36	with	40/60 kHz	806:814	arg1	MWs					832:834	the lowest MWs	821:834	the lowest MWs (0.85-14.93 × 105 Da)	821:856	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	37	theme	acid-binding	1222:1233	arg1	capacity					1235:1242	bile acid-binding capacity	1217:1242	bile acid-binding capacity	1217:1242	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	38	theme	carboxylate	1107:1117	arg1	%					1133:1133	76.08%	1128:1133	76.08%	1128:1133	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	38	theme	carboxylate	1107:1117	arg1	content					1119:1125	the highest carboxylate content	1095:1125	the highest carboxylate content (76.08%)	1095:1134	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	1	39	theme	in	363:364	arg1	activities					383:392	in vitro biological activities	363:392	in vitro biological activities	363:392	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	2	40	theme	OPSs	686:689	arg1	compositions					591:602	monosaccharide compositions	576:602	monosaccharide compositions	576:602	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	40	theme	OPSs	686:689	arg1	yields					547:552	extraction yields	536:552	extraction yields	536:552	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	40	theme	OPSs	686:689	arg1	morphologies					638:649	surface morphologies	630:649	surface morphologies	630:649	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	40	theme	OPSs	686:689	arg1	components					564:573	chemical components	555:573	chemical components	555:573	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	40	theme	OPSs	686:689	arg1	weights					615:621	molecular weights	605:621	molecular weights (MWs)	605:627	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	40	theme	OPSs	686:689	arg1	MWs					624:626	MWs	624:626	MWs	624:626	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	40	theme	OPSs	686:689	arg1	properties					668:677	rheological properties	656:677	rheological properties of the OPSs	656:689	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	3	41	from	40/60 kHz	806:814	arg1	DFU					799:801	DFU	799:801	DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures	799:933	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	42	theme	esterification	1177:1190	arg1	%					1133:1133	76.08%	1128:1133	76.08%	1128:1133	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	42	theme	esterification	1177:1190	arg1	MWs					1137:1139	MWs	1137:1139	MWs (7.28-32.83 × 105 Da)	1137:1161	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	42	theme	esterification	1177:1190	arg1	Da					1159:1160	7.28-32.83 × 105 Da	1142:1160	7.28-32.83 × 105 Da	1142:1160	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	42	theme	esterification	1177:1190	arg1	degree					1167:1172	degree	1167:1172	degree of esterification (30.7%)	1167:1198	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	42	theme	esterification	1177:1190	arg1	content					1119:1125	the highest carboxylate content	1095:1125	the highest carboxylate content (76.08%)	1095:1134	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	2	43	theme	chemical	555:562	arg1	components					564:573	chemical components	555:573	chemical components	555:573	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	44	from	frequencies	492:502	arg1	extractions					467:477	ultrasonic extractions	456:477	ultrasonic extractions at different frequencies	456:502	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	1	45	dep	dual-frequency	165:178	arg1	DFU					181:183	DFU	181:183	DFU	181:183	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	3	46	theme	loosest	908:914	arg1	structures					924:933	loosest network structures	908:933	40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures	806:933	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	47	theme	other	1034:1038	arg1	OPSs					1040:1043	the other OPSs	1030:1043	the other OPSs	1030:1043	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	1	48	theme	biological	372:381	arg1	activities					383:392	in vitro biological activities	363:392	in vitro biological activities	363:392	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	2	49	theme	surface	630:636	arg1	morphologies					638:649	surface morphologies	630:649	surface morphologies	630:649	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	50	from	variations	522:531	arg1	compositions					591:602	monosaccharide compositions	576:602	monosaccharide compositions	576:602	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	50	from	variations	522:531	arg1	yields					547:552	extraction yields	536:552	extraction yields	536:552	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	50	from	variations	522:531	arg1	morphologies					638:649	surface morphologies	630:649	surface morphologies	630:649	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	50	from	variations	522:531	arg1	components					564:573	chemical components	555:573	chemical components	555:573	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	50	from	variations	522:531	arg1	weights					615:621	molecular weights	605:621	molecular weights (MWs)	605:627	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	50	from	variations	522:531	arg1	MWs					624:626	MWs	624:626	MWs	624:626	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	2	50	from	variations	522:531	arg1	properties					668:677	rheological properties	656:677	rheological properties of the OPSs	656:689	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	3	51	theme	network	916:922	arg1	structures					924:933	loosest network structures	908:933	40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures	806:933	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	2	52	theme	preliminary	717:727	arg1	features					740:747	their preliminary structural features	711:747	their preliminary structural features	711:747	Results showed that ultrasonic extractions at different frequencies led to remarkable variations in extraction yields, chemical components, monosaccharide compositions, molecular weights (MWs), surface morphologies, and rheological properties of the OPSs but hardly affected their preliminary structural features and thermal stabilities.					
34062454	3	53	theme	other	1253:1257	arg1	OPSs					1259:1262	the other OPSs	1249:1262	the other OPSs	1249:1262	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	1	54	dep	in	363:364	arg1	vitro					366:370	vitro	366:370	vitro	366:370	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	3	55	theme	ion	999:1001	arg1	capacities					1014:1023	nitrite ion absorption capacities	991:1023	nitrite ion absorption capacities	991:1023	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	56	theme	cholesterol	964:974	arg1	absorption					976:985	cholesterol absorption	964:985	cholesterol absorption	964:985	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	1	57	theme	single-	147:153	arg1	ultrasounds					186:196	single- (SFU) and dual-frequency (DFU) ultrasounds	147:196	single- (SFU) and dual-frequency (DFU) ultrasounds	147:196	In this study, single- (SFU) and dual-frequency (DFU) ultrasounds were used to extract polysaccharides from okra (Abelmoschus esculentus (L.) Moench) pods (OPSs), and the physicochemical characteristics, functional properties, and in vitro biological activities of the OPSs were comparatively evaluated.					
34062454	3	58	from	20 kHz	1083:1088	arg1	SFU					1076:1078	SFU	1076:1078	SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%)	1076:1198	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	0	59	from	characteristics	31:45	arg1	okra					71:74	okra	71:74	okra	71:74	Physicochemical and functional characteristics of polysaccharides from okra extracted by using ultrasound at different frequencies.					
34062454	3	60	theme	superior	942:949	arg1	antioxidant					951:961	superior antioxidant	942:961	superior antioxidant	942:961	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	61	theme	highest	862:868	arg1	content					881:887	highest polyphenol content	862:887	highest polyphenol content (7.38%)	862:895	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
34062454	3	61	theme	highest	862:868	arg1	%					894:894	7.38%	890:894	7.38%	890:894	The OPS obtained through DFU at 40/60 kHz with the lowest MWs (0.85-14.93 × 105 Da) and highest polyphenol content (7.38%) as well as loosest network structures showed superior antioxidant, cholesterol absorption and nitrite ion absorption capacities than the other OPSs, and the OPS extracted through SFU at 20 kHz with the highest carboxylate content (76.08%), MWs (7.28-32.83 × 105 Da) and degree of esterification (30.7%) exhibited higher bile acid-binding capacity than the other OPSs.					
32507188	0	0	theme	Gram-negative	100:112	arg1	bacteria					132:139	both Gram-negative and Gram-positive bacteria	95:139	both Gram-negative and Gram-positive bacteria	95:139	Drug-free and non-crosslinked chitosan scaffolds with efficient antibacterial activity against both Gram-negative and Gram-positive bacteria.					
32507188	5	1	theme	1 mg	711:714	arg1	scaffolds					719:727	1 mg CS scaffolds	711:727	1 mg CS scaffolds	711:727	We found both pathogens were completely killed by 1 mg CS scaffolds at 6 h, due largely to the CS-induced time-dependent bacteria clustering.					
32507188	7	2	theme	first	976:980	arg1	time					982:985	the first time	972:985	the first time	972:985	In conclusion, the bactericidal activity of CS scaffolds alone is reported for the first time.					
32507188	4	3	theme	Streptococcus	639:651	arg1	mutans					653:658	Gram-positive Streptococcus mutans	625:658	Gram-positive Streptococcus mutans	625:658	Herein, we fabricated the non-crosslinked CS scaffold and investigated its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans.					
32507188	5	4	theme	CS	716:717	arg1	scaffolds					719:727	1 mg CS scaffolds	711:727	1 mg CS scaffolds	711:727	We found both pathogens were completely killed by 1 mg CS scaffolds at 6 h, due largely to the CS-induced time-dependent bacteria clustering.					
32507188	8	5	theme	antibacterial	1071:1083	arg1	activity					1085:1092	great antibacterial activity	1065:1092	great antibacterial activity	1065:1092	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	4	6	theme	Gram-positive	625:637	arg1	mutans					653:658	Gram-positive Streptococcus mutans	625:658	Gram-positive Streptococcus mutans	625:658	Herein, we fabricated the non-crosslinked CS scaffold and investigated its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans.					
32507188	0	7	theme	Gram-positive	118:130	arg1	bacteria					132:139	both Gram-negative and Gram-positive bacteria	95:139	both Gram-negative and Gram-positive bacteria	95:139	Drug-free and non-crosslinked chitosan scaffolds with efficient antibacterial activity against both Gram-negative and Gram-positive bacteria.					
32507188	3	8	theme	scaffolds	411:419	arg1	activity					396:403	the intrinsic antibacterial activity	368:403	the intrinsic antibacterial activity of CS scaffolds	368:419	However, the intrinsic antibacterial activity of CS scaffolds alone has never been reported.					
32507188	0	9	link	non-crosslinked	14:28	arg1	chitosan					30:37	Drug-free and non-crosslinked chitosan	0:37	Drug-free and non-crosslinked chitosan	0:37	Drug-free and non-crosslinked chitosan scaffolds with efficient antibacterial activity against both Gram-negative and Gram-positive bacteria.					
32507188	3	10	theme	antibacterial	382:394	arg1	activity					396:403	the intrinsic antibacterial activity	368:403	the intrinsic antibacterial activity of CS scaffolds	368:419	However, the intrinsic antibacterial activity of CS scaffolds alone has never been reported.					
32507188	8	11	theme	great	1137:1141	arg1	potentials					1143:1152	great potentials	1137:1152	great potentials	1137:1152	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	4	12	theme	CS	494:495	arg1	scaffold					497:504	the non-crosslinked CS scaffold	474:504	the non-crosslinked CS scaffold	474:504	Herein, we fabricated the non-crosslinked CS scaffold and investigated its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans.					
32507188	1	13	theme	oral	192:195	arg1	health					210:215	both oral and systemic health	187:215	both oral and systemic health	187:215	Treatment of oral pathogens is important for both oral and systemic health.					
32507188	8	14	theme	non-crosslinked	1099:1113	arg1	scaffolds					1118:1126	the non-crosslinked CS scaffolds	1095:1126	the non-crosslinked CS scaffolds	1095:1126	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	2	15	theme	loading	285:291	arg1	antibiotics					293:303	loading antibiotics	285:303	loading antibiotics	285:303	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents are well documented.					
32507188	7	16	theme	CS	937:938	arg1	scaffolds					940:948	CS scaffolds	937:948	CS scaffolds	937:948	In conclusion, the bactericidal activity of CS scaffolds alone is reported for the first time.					
32507188	3	17	theme	CS	408:409	arg1	scaffolds					411:419	CS scaffolds	408:419	CS scaffolds	408:419	However, the intrinsic antibacterial activity of CS scaffolds alone has never been reported.					
32507188	6	18	theme	antibacterial	869:881	arg1	activity					883:890	no antibacterial activity	866:890	no antibacterial activity	866:890	Interestingly, β-glycerophosphate crosslinked scaffolds showed no antibacterial activity.					
32507188	7	19	theme	scaffolds	940:948	arg1	activity					925:932	the bactericidal activity	908:932	the bactericidal activity of CS scaffolds	908:948	In conclusion, the bactericidal activity of CS scaffolds alone is reported for the first time.					
32507188	1	20	theme	systemic	201:208	arg1	health					210:215	both oral and systemic health	187:215	both oral and systemic health	187:215	Treatment of oral pathogens is important for both oral and systemic health.					
32507188	0	21	theme	Drug-free	0:8	arg1	chitosan					30:37	Drug-free and non-crosslinked chitosan	0:37	Drug-free and non-crosslinked chitosan	0:37	Drug-free and non-crosslinked chitosan scaffolds with efficient antibacterial activity against both Gram-negative and Gram-positive bacteria.					
32507188	4	22	theme	Porphyromonas	596:608	arg1	gingivalis					610:619	Gram-negative Porphyromonas gingivalis	582:619	Gram-negative Porphyromonas gingivalis	582:619	Herein, we fabricated the non-crosslinked CS scaffold and investigated its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans.					
32507188	5	23	theme	bacteria	782:789	arg1	clustering					791:800	the CS-induced time-dependent bacteria clustering	752:800	the CS-induced time-dependent bacteria clustering	752:800	We found both pathogens were completely killed by 1 mg CS scaffolds at 6 h, due largely to the CS-induced time-dependent bacteria clustering.					
32507188	2	24	dep	scaffolds	268:276	arg1	either					278:283	either	278:283	either	278:283	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents are well documented.					
32507188	4	25	theme	Gram-negative	582:594	arg1	gingivalis					610:619	Gram-negative Porphyromonas gingivalis	582:619	Gram-negative Porphyromonas gingivalis	582:619	Herein, we fabricated the non-crosslinked CS scaffold and investigated its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans.					
32507188	8	26	theme	CS	1115:1116	arg1	scaffolds					1118:1126	the non-crosslinked CS scaffolds	1095:1126	the non-crosslinked CS scaffolds	1095:1126	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	2	27	theme	other	325:329	arg1	agents					331:336	other agents	325:336	other agents	325:336	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents are well documented.					
32507188	0	28	theme	non-crosslinked	14:28	arg1	chitosan					30:37	Drug-free and non-crosslinked chitosan	0:37	Drug-free and non-crosslinked chitosan	0:37	Drug-free and non-crosslinked chitosan scaffolds with efficient antibacterial activity against both Gram-negative and Gram-positive bacteria.					
32507188	8	29	theme	oral	1175:1178	arg1	diseases					1180:1187	oral diseases	1175:1187	oral diseases	1175:1187	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	5	30	theme	CS-induced	756:765	arg1	clustering					791:800	the CS-induced time-dependent bacteria clustering	752:800	the CS-induced time-dependent bacteria clustering	752:800	We found both pathogens were completely killed by 1 mg CS scaffolds at 6 h, due largely to the CS-induced time-dependent bacteria clustering.					
32507188	7	31	theme	bactericidal	912:923	arg1	activity					925:932	the bactericidal activity	908:932	the bactericidal activity of CS scaffolds	908:948	In conclusion, the bactericidal activity of CS scaffolds alone is reported for the first time.					
32507188	4	32	theme	oral	566:569	arg1	pathogens					571:579	typical oral pathogens	558:579	typical oral pathogens	558:579	Herein, we fabricated the non-crosslinked CS scaffold and investigated its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans.					
32507188	5	33	theme	time-dependent	767:780	arg1	clustering					791:800	the CS-induced time-dependent bacteria clustering	752:800	the CS-induced time-dependent bacteria clustering	752:800	We found both pathogens were completely killed by 1 mg CS scaffolds at 6 h, due largely to the CS-induced time-dependent bacteria clustering.					
32507188	4	34	theme	non-crosslinked	478:492	arg1	scaffold					497:504	the non-crosslinked CS scaffold	474:504	the non-crosslinked CS scaffold	474:504	Herein, we fabricated the non-crosslinked CS scaffold and investigated its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans.					
32507188	8	35	contain	have	1132:1135	arg2	potentials					1143:1152	great potentials	1137:1152	great potentials	1137:1152	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	8	35	contain	have	1132:1135	arg1	scaffolds					1118:1126	the non-crosslinked CS scaffolds	1095:1126	the non-crosslinked CS scaffolds	1095:1126	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	0	36	theme	antibacterial	64:76	arg1	activity					78:85	efficient antibacterial activity	54:85	efficient antibacterial activity against both Gram-negative and Gram-positive bacteria	54:139	Drug-free and non-crosslinked chitosan scaffolds with efficient antibacterial activity against both Gram-negative and Gram-positive bacteria.					
32507188	3	37	theme	intrinsic	372:380	arg1	activity					396:403	the intrinsic antibacterial activity	368:403	the intrinsic antibacterial activity of CS scaffolds	368:419	However, the intrinsic antibacterial activity of CS scaffolds alone has never been reported.					
32507188	8	38	theme	wound	1201:1205	arg1	healing					1207:1213	wound healing	1201:1213	wound healing	1201:1213	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	8	39	theme	tissue	1219:1224	arg1	engineering					1226:1236	tissue engineering	1219:1236	tissue engineering	1219:1236	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	2	40	theme	chitosan	248:255	arg1	scaffolds					268:276	chitosan (CS)-based scaffolds	248:276	chitosan (CS)-based scaffolds	248:276	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents are well documented.					
32507188	6	41	theme	crosslinked	837:847	arg1	scaffolds					849:857	β-glycerophosphate crosslinked scaffolds	818:857	β-glycerophosphate crosslinked scaffolds	818:857	Interestingly, β-glycerophosphate crosslinked scaffolds showed no antibacterial activity.					
32507188	0	42	theme	efficient	54:62	arg1	activity					78:85	efficient antibacterial activity	54:85	efficient antibacterial activity against both Gram-negative and Gram-positive bacteria	54:139	Drug-free and non-crosslinked chitosan scaffolds with efficient antibacterial activity against both Gram-negative and Gram-positive bacteria.					
32507188	8	43	theme	physical	1024:1031	arg1	stability					1033:1041	physical stability	1024:1041	physical stability	1024:1041	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	6	44	theme	β-glycerophosphate	818:835	arg1	scaffolds					849:857	β-glycerophosphate crosslinked scaffolds	818:857	β-glycerophosphate crosslinked scaffolds	818:857	Interestingly, β-glycerophosphate crosslinked scaffolds showed no antibacterial activity.					
32507188	5	45	theme	due	737:739	arg1	6 h					732:734	6 h	732:734	6 h	732:734	We found both pathogens were completely killed by 1 mg CS scaffolds at 6 h, due largely to the CS-induced time-dependent bacteria clustering.					
32507188	2	46	theme	antimicrobial	222:234	arg1	activity					236:243	The antimicrobial activity	218:243	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents	218:336	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents are well documented.					
32507188	8	47	theme	great	1065:1069	arg1	activity					1085:1092	great antibacterial activity	1065:1092	great antibacterial activity	1065:1092	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	1	48	theme	oral	155:158	arg1	pathogens					160:168	oral pathogens	155:168	oral pathogens	155:168	Treatment of oral pathogens is important for both oral and systemic health.					
32507188	2	49	with	antibiotics	293:303	arg1	agents					331:336	other agents	325:336	other agents	325:336	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents are well documented.					
32507188	2	50	theme	scaffolds	268:276	arg1	activity					236:243	The antimicrobial activity	218:243	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents	218:336	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents are well documented.					
32507188	1	51	theme	pathogens	160:168	arg1	Treatment					142:150	Treatment	142:150	Treatment of oral pathogens	142:168	Treatment of oral pathogens is important for both oral and systemic health.					
32507188	2	52	theme	-based	261:266	arg1	scaffolds					268:276	chitosan (CS)-based scaffolds	248:276	chitosan (CS)-based scaffolds	248:276	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents are well documented.					
32507188	2	53	with	compositing	308:318	arg1	agents					331:336	other agents	325:336	other agents	325:336	The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents are well documented.					
32507188	8	54	link	non-crosslinked	1099:1113	arg1	scaffolds					1118:1126	the non-crosslinked CS scaffolds	1095:1126	the non-crosslinked CS scaffolds	1095:1126	Together with the biodegradability, physical stability, biocompatibility and great antibacterial activity, the non-crosslinked CS scaffolds may have great potentials not only in treating oral diseases but also in wound healing and tissue engineering.					
32507188	4	55	theme	antibacterial	527:539	arg1	activity					541:548	its antibacterial activity	523:548	its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans	523:658	Herein, we fabricated the non-crosslinked CS scaffold and investigated its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans.					
32507188	6	56	link	crosslinked	837:847	arg1	scaffolds					849:857	β-glycerophosphate crosslinked scaffolds	818:857	β-glycerophosphate crosslinked scaffolds	818:857	Interestingly, β-glycerophosphate crosslinked scaffolds showed no antibacterial activity.					
32507188	4	57	link	non-crosslinked	478:492	arg1	scaffold					497:504	the non-crosslinked CS scaffold	474:504	the non-crosslinked CS scaffold	474:504	Herein, we fabricated the non-crosslinked CS scaffold and investigated its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans.					
32507188	4	58	theme	typical	558:564	arg1	pathogens					571:579	typical oral pathogens	558:579	typical oral pathogens	558:579	Herein, we fabricated the non-crosslinked CS scaffold and investigated its antibacterial activity against typical oral pathogens, Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans.					
32604764	4	0	theme	non-extruded	824:835	arg1	sample					837:842	the non-extruded sample	820:842	the non-extruded sample	820:842	The extrusion pretreatment can increase the extraction yield of fucoidan by approximately 4.2-fold as compared to the non-extruded sample.					
32604764	6	1	theme	lung	1003:1006	arg1	cells					1024:1028	human lung carcinoma A-549 cells	997:1028	human lung carcinoma A-549 cells	997:1028	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	8	2	theme	late	1479:1482	arg1	apoptosis					1484:1492	late apoptosis	1479:1492	late apoptosis of A-549 cells	1479:1507	SCA also showed high efficacy to induce cytochrome c release, activate caspase-9 and -3, and promote late apoptosis of A-549 cells.					
32604764	3	3	theme	SC	649:650	arg1	degradation					634:644	degradation	634:644	degradation of SC by ascorbic acid	634:667	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	6	4	theme	c	1166:1166	arg1	release					1168:1174	cytochrome c release	1155:1174	cytochrome c release	1155:1174	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	3	5	theme	SC	556:557	arg1	degradation					541:551	degradation	541:551	degradation of SC by ascorbic acid	541:574	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	6	6	theme	potential	1080:1088	arg1	loss					1049:1052	loss	1049:1052	loss of mitochondrial membrane potential (MMP)	1049:1094	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	10	7	theme	SCH-	1745:1748	arg1	apoptosis					1768:1776	SC-, SCA-, SCH-, and SCAH-induced apoptosis	1734:1776	SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells	1734:1791	Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.					
32604764	3	8	dep	SCH	578:580	arg1	degradation					583:593	degradation	583:593	degradation of SC by hydrogen peroxide	583:620	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	6	9	theme	mitochondrial	1057:1069	arg1	potential					1080:1088	mitochondrial membrane potential	1057:1088	mitochondrial membrane potential (MMP)	1057:1094	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	6	9	theme	mitochondrial	1057:1069	arg1	MMP					1091:1093	MMP	1091:1093	MMP	1091:1093	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	10	10	theme	SC-	1734:1736	arg1	apoptosis					1768:1776	SC-, SCA-, SCH-, and SCAH-induced apoptosis	1734:1776	SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells	1734:1791	Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.					
32604764	10	11	theme	A-549	1781:1785	arg1	cells					1787:1791	A-549 cells	1781:1791	A-549 cells	1781:1791	Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.					
32604764	3	12	theme	ascorbic	562:569	arg1	acid					571:574	ascorbic acid	562:574	ascorbic acid	562:574	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	0	13	theme	In	122:123	arg1	Analyses					148:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses	91:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products	91:183	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	1	14	theme	biological	213:222	arg1	functions					224:232	multiple biological functions	204:232	multiple biological functions including anti-cancer activity	204:263	Fucoidans possess multiple biological functions including anti-cancer activity.					
32604764	1	14	theme	biological	213:222	arg1	activity					256:263	anti-cancer activity	244:263	anti-cancer activity	244:263	Fucoidans possess multiple biological functions including anti-cancer activity.					
32604764	0	15	theme	Anti-Lung	131:139	arg1	Analyses					148:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses	91:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products	91:183	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	9	16	theme	therapeutic	1570:1580	arg1	potential					1582:1590	an adjuvant therapeutic potential	1558:1590	an adjuvant therapeutic potential	1558:1590	Therefore, our data suggest that SCA could have an adjuvant therapeutic potential in the treatment of lung cancer.					
32604764	6	17	theme	B-cell	1107:1112	arg1	expression					1133:1142	B-cell leukemia-2 (Bcl-2) expression	1107:1142	B-cell leukemia-2 (Bcl-2) expression	1107:1142	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	6	18	theme	active	1187:1192	arg1	caspase-9					1194:1202	active caspase-9	1187:1202	active caspase-9	1187:1202	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	3	19	theme	hydrogen	604:611	arg1	peroxide					613:620	hydrogen peroxide	604:620	hydrogen peroxide	604:620	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	0	20	theme	Sargassum	15:23	arg1	Fucoidan					38:45	Sargassum crassifolium Fucoidan	15:45	Sargassum crassifolium Fucoidan	15:45	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	0	21	dep	In	122:123	arg1	Vitro					125:129	Vitro	125:129	Vitro	125:129	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	0	22	theme	Products	176:183	arg1	Peroxide					77:84	Hydrogen Peroxide	68:84	Hydrogen Peroxide	68:84	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	0	22	theme	Products	176:183	arg1	Analyses					148:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses	91:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products	91:183	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	0	22	theme	Products	176:183	arg1	Acid					59:62	Ascorbic Acid	50:62	Ascorbic Acid	50:62	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	9	23	theme	lung	1612:1615	arg1	cancer					1617:1622	lung cancer	1612:1622	lung cancer	1612:1622	Therefore, our data suggest that SCA could have an adjuvant therapeutic potential in the treatment of lung cancer.					
32604764	4	24	theme	extrusion	710:718	arg1	pretreatment					720:731	The extrusion pretreatment	706:731	The extrusion pretreatment	706:731	The extrusion pretreatment can increase the extraction yield of fucoidan by approximately 4.2-fold as compared to the non-extruded sample.					
32604764	5	25	theme	chemical	879:886	arg1	compositions					888:899	the chemical compositions	875:899	the chemical compositions	875:899	Among SC, SCA, SCH, and SCAH, the chemical compositions varied but structural features were similar.					
32604764	6	26	theme	A-549	1244:1248	arg1	cells					1250:1254	A-549 cells	1244:1254	A-549 cells	1244:1254	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	5	27	theme	structural	912:921	arg1	features					923:930	structural features	912:930	structural features	912:930	Among SC, SCA, SCH, and SCAH, the chemical compositions varied but structural features were similar.					
32604764	8	28	theme	high	1394:1397	arg1	efficacy					1399:1406	high efficacy	1394:1406	high efficacy	1394:1406	SCA also showed high efficacy to induce cytochrome c release, activate caspase-9 and -3, and promote late apoptosis of A-549 cells.					
32604764	4	29	dep	4.2-fold	796:803	arg1	compared					808:815	compared	808:815	compared to the non-extruded sample	808:842	The extrusion pretreatment can increase the extraction yield of fucoidan by approximately 4.2-fold as compared to the non-extruded sample.					
32604764	3	30	dep	SCA	536:538	arg1	degradation					541:551	degradation	541:551	degradation of SC by ascorbic acid	541:574	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	8	31	theme	cytochrome	1418:1427	arg1	release					1431:1437	cytochrome c release	1418:1437	cytochrome c release	1418:1437	SCA also showed high efficacy to induce cytochrome c release, activate caspase-9 and -3, and promote late apoptosis of A-549 cells.					
32604764	4	32	theme	fucoidan	770:777	arg1	yield					761:765	the extraction yield	746:765	the extraction yield of fucoidan	746:777	The extrusion pretreatment can increase the extraction yield of fucoidan by approximately 4.2-fold as compared to the non-extruded sample.					
32604764	0	33	theme	Fucoidan	38:45	arg1	Degradation					0:10	Degradation	0:10	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.	0:184	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	2	34	contain	possess	323:329	arg1	fucoidans					297:305	low-molecular-weight fucoidans	276:305	low-molecular-weight fucoidans	276:305	Moreover, low-molecular-weight fucoidans are reported to possess more bioactivities than native fucoidans.					
32604764	2	34	contain	possess	323:329	arg2	bioactivities					336:348	more bioactivities	331:348	more bioactivities	331:348	Moreover, low-molecular-weight fucoidans are reported to possess more bioactivities than native fucoidans.					
32604764	10	35	theme	rapamycin	1684:1692	arg1	pathway					1711:1717	rapamycin (mTOR) signaling pathway	1684:1717	rapamycin (mTOR) signaling pathway	1684:1717	Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.					
32604764	9	36	contain	have	1553:1556	arg2	potential					1582:1590	an adjuvant therapeutic potential	1558:1590	an adjuvant therapeutic potential	1558:1590	Therefore, our data suggest that SCA could have an adjuvant therapeutic potential in the treatment of lung cancer.					
32604764	9	36	contain	have	1553:1556	arg1	SCA					1543:1545	SCA	1543:1545	SCA	1543:1545	Therefore, our data suggest that SCA could have an adjuvant therapeutic potential in the treatment of lung cancer.					
32604764	0	37	theme	Ascorbic	50:57	arg1	Acid					59:62	Ascorbic Acid	50:62	Ascorbic Acid	50:62	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	3	38	theme	SC	598:599	arg1	degradation					583:593	degradation	583:593	degradation of SC by hydrogen peroxide	583:620	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	8	39	theme	A-549	1497:1501	arg1	cells					1503:1507	A-549 cells	1497:1507	A-549 cells	1497:1507	SCA also showed high efficacy to induce cytochrome c release, activate caspase-9 and -3, and promote late apoptosis of A-549 cells.					
32604764	6	40	theme	membrane	1071:1078	arg1	potential					1080:1088	mitochondrial membrane potential	1057:1088	mitochondrial membrane potential (MMP)	1057:1094	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	6	40	theme	membrane	1071:1078	arg1	MMP					1091:1093	MMP	1091:1093	MMP	1091:1093	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	7	41	theme	Bcl-2	1360:1364	arg1	expression					1366:1375	Bcl-2 expression	1360:1375	Bcl-2 expression	1360:1375	In general, SCA was found to exhibit high cytotoxicity to A-549 cells and a strong ability to suppress Bcl-2 expression.					
32604764	10	42	theme	signaling	1701:1709	arg1	pathway					1711:1717	rapamycin (mTOR) signaling pathway	1684:1717	rapamycin (mTOR) signaling pathway	1684:1717	Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.					
32604764	1	43	theme	anti-cancer	244:254	arg1	activity					256:263	anti-cancer activity	244:263	anti-cancer activity	244:263	Fucoidans possess multiple biological functions including anti-cancer activity.					
32604764	6	44	theme	carcinoma	1008:1016	arg1	cells					1024:1028	human lung carcinoma A-549 cells	997:1028	human lung carcinoma A-549 cells	997:1028	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	0	45	theme	Degradation	164:174	arg1	Products					176:183	the Degradation Products	160:183	the Degradation Products	160:183	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	3	46	dep	SCAH	628:631	arg1	peroxide					680:687	hydrogen peroxide	671:687	hydrogen peroxide	671:687	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	3	46	dep	SCAH	628:631	arg1	degradation					634:644	degradation	634:644	degradation of SC by ascorbic acid	634:667	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	7	47	theme	strong	1333:1338	arg1	ability					1340:1346	a strong ability	1331:1346	a strong ability to suppress Bcl-2 expression	1331:1375	In general, SCA was found to exhibit high cytotoxicity to A-549 cells and a strong ability to suppress Bcl-2 expression.					
32604764	6	48	theme	human	997:1001	arg1	cells					1024:1028	human lung carcinoma A-549 cells	997:1028	human lung carcinoma A-549 cells	997:1028	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	3	49	theme	degraded	508:515	arg1	fucoidans					517:525	three degraded fucoidans	502:525	three degraded fucoidans	502:525	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	3	49	theme	degraded	508:515	arg1	SCH					578:580	SCH	578:580	SCH (degradation of SC by hydrogen peroxide)	578:621	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	3	49	theme	degraded	508:515	arg1	SCAH					628:631	SCAH	628:631	SCAH (degradation of SC by ascorbic acid + hydrogen peroxide)	628:688	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	3	49	theme	degraded	508:515	arg1	SCA					536:538	SCA	536:538	SCA (degradation of SC by ascorbic acid)	536:575	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	6	50	theme	cytochrome	1155:1164	arg1	release					1168:1174	cytochrome c release	1155:1174	cytochrome c release	1155:1174	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	6	51	from	effects	986:992	arg1	cells					1024:1028	human lung carcinoma A-549 cells	997:1028	human lung carcinoma A-549 cells	997:1028	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	7	52	theme	A-549	1315:1319	arg1	cells					1321:1325	A-549 cells	1315:1325	A-549 cells	1315:1325	In general, SCA was found to exhibit high cytotoxicity to A-549 cells and a strong ability to suppress Bcl-2 expression.					
32604764	7	53	theme	high	1294:1297	arg1	cytotoxicity					1299:1310	high cytotoxicity	1294:1310	high cytotoxicity to A-549 cells	1294:1325	In general, SCA was found to exhibit high cytotoxicity to A-549 cells and a strong ability to suppress Bcl-2 expression.					
32604764	3	54	theme	hydrogen	671:678	arg1	peroxide					680:687	hydrogen peroxide	671:687	hydrogen peroxide	671:687	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	6	55	theme	late	1226:1229	arg1	apoptosis					1231:1239	late apoptosis	1226:1239	late apoptosis of A-549 cells	1226:1254	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	2	56	theme	more	331:334	arg1	bioactivities					336:348	more bioactivities	331:348	more bioactivities	331:348	Moreover, low-molecular-weight fucoidans are reported to possess more bioactivities than native fucoidans.					
32604764	10	57	theme	SCAH-induced	1755:1766	arg1	apoptosis					1768:1776	SC-, SCA-, SCH-, and SCAH-induced apoptosis	1734:1776	SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells	1734:1791	Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.					
32604764	1	58	theme	multiple	204:211	arg1	functions					224:232	multiple biological functions	204:232	multiple biological functions including anti-cancer activity	204:263	Fucoidans possess multiple biological functions including anti-cancer activity.					
32604764	1	58	theme	multiple	204:211	arg1	activity					256:263	anti-cancer activity	244:263	anti-cancer activity	244:263	Fucoidans possess multiple biological functions including anti-cancer activity.					
32604764	6	59	theme	Bcl-2	1126:1130	arg1	expression					1133:1142	B-cell leukemia-2 (Bcl-2) expression	1107:1142	B-cell leukemia-2 (Bcl-2) expression	1107:1142	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	6	60	theme	cells	1250:1254	arg1	apoptosis					1231:1239	late apoptosis	1226:1239	late apoptosis of A-549 cells	1226:1254	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	0	61	theme	Cancer	141:146	arg1	Analyses					148:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses	91:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products	91:183	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	9	62	theme	adjuvant	1561:1568	arg1	potential					1582:1590	an adjuvant therapeutic potential	1558:1590	an adjuvant therapeutic potential	1558:1590	Therefore, our data suggest that SCA could have an adjuvant therapeutic potential in the treatment of lung cancer.					
32604764	6	63	theme	leukemia-2	1114:1123	arg1	expression					1133:1142	B-cell leukemia-2 (Bcl-2) expression	1107:1142	B-cell leukemia-2 (Bcl-2) expression	1107:1142	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	10	64	theme	cells	1787:1791	arg1	apoptosis					1768:1776	SC-, SCA-, SCH-, and SCAH-induced apoptosis	1734:1776	SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells	1734:1791	Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.					
32604764	2	65	theme	low-molecular-weight	276:295	arg1	fucoidans					297:305	low-molecular-weight fucoidans	276:305	low-molecular-weight fucoidans	276:305	Moreover, low-molecular-weight fucoidans are reported to possess more bioactivities than native fucoidans.					
32604764	0	66	theme	Hydrogen	68:75	arg1	Peroxide					77:84	Hydrogen Peroxide	68:84	Hydrogen Peroxide	68:84	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	10	67	theme	Akt/mammalian	1660:1672	arg1	target					1674:1679	the Akt/mammalian target	1656:1679	the Akt/mammalian target of rapamycin (mTOR) signaling pathway	1656:1717	Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.					
32604764	1	68	contain	possess	196:202	arg2	functions					224:232	multiple biological functions	204:232	multiple biological functions including anti-cancer activity	204:263	Fucoidans possess multiple biological functions including anti-cancer activity.					
32604764	1	68	contain	possess	196:202	arg2	activity					256:263	anti-cancer activity	244:263	anti-cancer activity	244:263	Fucoidans possess multiple biological functions including anti-cancer activity.					
32604764	1	68	contain	possess	196:202	arg1	Fucoidans					186:194	Fucoidans	186:194	Fucoidans	186:194	Fucoidans possess multiple biological functions including anti-cancer activity.					
32604764	0	69	theme	Compositional	91:103	arg1	Analyses					148:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses	91:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products	91:183	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	9	70	theme	cancer	1617:1622	arg1	treatment					1599:1607	the treatment	1595:1607	the treatment of lung cancer	1595:1622	Therefore, our data suggest that SCA could have an adjuvant therapeutic potential in the treatment of lung cancer.					
32604764	0	71	theme	Structural	106:115	arg1	Analyses					148:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses	91:155	Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products	91:183	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	3	72	theme	present	380:386	arg1	study					388:392	the present study	376:392	the present study	376:392	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	6	73	theme	apoptotic	976:984	arg1	effects					986:992	apoptotic effects	976:992	apoptotic effects on human lung carcinoma A-549 cells	976:1028	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	6	74	dep	showed	969:974	arg1	increased					1145:1153	increased	1145:1153	increased cytochrome c release	1145:1174	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	6	74	dep	showed	969:974	arg1	decreased					1097:1105	decreased	1097:1105	decreased B-cell leukemia-2 (Bcl-2) expression	1097:1142	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	6	74	dep	showed	969:974	arg1	increased					1216:1224	increased	1216:1224	increased late apoptosis of A-549 cells	1216:1254	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	6	74	dep	showed	969:974	arg1	increased					1177:1185	increased	1177:1185	increased active caspase-9 and -3	1177:1209	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	0	75	theme	crassifolium	25:36	arg1	Fucoidan					38:45	Sargassum crassifolium Fucoidan	15:45	Sargassum crassifolium Fucoidan	15:45	Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products.					
32604764	7	76	located	found	1277:1281	arg2	SCA					1269:1271	SCA	1269:1271	SCA	1269:1271	In general, SCA was found to exhibit high cytotoxicity to A-549 cells and a strong ability to suppress Bcl-2 expression.					
32604764	7	76	located	found	1277:1281	arg1	general					1260:1266	general	1260:1266	general	1260:1266	In general, SCA was found to exhibit high cytotoxicity to A-549 cells and a strong ability to suppress Bcl-2 expression.					
32604764	3	77	theme	native	397:402	arg1	fucoidan					404:411	a native fucoidan	395:411	a native fucoidan (SC)	395:416	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	3	77	theme	native	397:402	arg1	SC					414:415	SC	414:415	SC	414:415	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	8	78	theme	c	1429:1429	arg1	release					1431:1437	cytochrome c release	1418:1437	cytochrome c release	1418:1437	SCA also showed high efficacy to induce cytochrome c release, activate caspase-9 and -3, and promote late apoptosis of A-549 cells.					
32604764	3	79	theme	single-screw	474:485	arg1	extrusion					487:495	single-screw extrusion	474:495	single-screw extrusion	474:495	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	3	80	theme	ascorbic	655:662	arg1	acid					664:667	ascorbic acid	655:667	ascorbic acid	655:667	In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced.					
32604764	8	81	theme	cells	1503:1507	arg1	apoptosis					1484:1492	late apoptosis	1479:1492	late apoptosis of A-549 cells	1479:1507	SCA also showed high efficacy to induce cytochrome c release, activate caspase-9 and -3, and promote late apoptosis of A-549 cells.					
32604764	10	82	theme	pathway	1711:1717	arg1	target					1674:1679	the Akt/mammalian target	1656:1679	the Akt/mammalian target of rapamycin (mTOR) signaling pathway	1656:1717	Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.					
32604764	2	83	theme	native	355:360	arg1	fucoidans					362:370	native fucoidans	355:370	native fucoidans	355:370	Moreover, low-molecular-weight fucoidans are reported to possess more bioactivities than native fucoidans.					
32604764	6	84	theme	A-549	1018:1022	arg1	cells					1024:1028	human lung carcinoma A-549 cells	997:1028	human lung carcinoma A-549 cells	997:1028	SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells.					
32604764	10	85	theme	SCA-	1739:1742	arg1	apoptosis					1768:1776	SC-, SCA-, SCH-, and SCAH-induced apoptosis	1734:1776	SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells	1734:1791	Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells.					
32604764	4	86	theme	extraction	750:759	arg1	yield					761:765	the extraction yield	746:765	the extraction yield of fucoidan	746:777	The extrusion pretreatment can increase the extraction yield of fucoidan by approximately 4.2-fold as compared to the non-extruded sample.					
34455316	5	0	theme	pH	1096:1097	arg1	sensitivity					1099:1109	pH sensitivity	1096:1109	pH sensitivity	1096:1109	In this hydrogel system, CBSP as the backbone material, was negatively charged and conferred the hydrogel with pH sensitivity.					
34455316	4	1	theme	natural	721:727	arg1	glucomannan					729:739	natural glucomannan	721:739	natural glucomannan with diverse bioactivities and biocompatibility	721:787	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	6	2	theme	catechol	1128:1135	arg1	groups					1137:1142	catechol groups	1128:1142	catechol groups	1128:1142	The presence of catechol groups greatly enhanced the tissue adhesion and antioxidant capacities of the hydrogel.					
34455316	4	3	theme	developed	635:643	arg1	work					645:648	this developed work	630:648	this developed work	630:648	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	4	theme	DA	913:914	arg1	conjugate					917:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate	876:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties	876:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	3	5	theme	effective	553:561	arg1	strategy					563:570	a simple and effective strategy	540:570	a simple and effective strategy to develop novel multifunctional smart hydrogels	540:619	However, limitations owing to their complex compositions and mechanisms, cumbersome synthetic routes, and single function call for a simple and effective strategy to develop novel multifunctional smart hydrogels.					
34455316	1	6	theme	internal	275:282	arg1	stimuli					284:290	external and internal stimuli	262:290	external and internal stimuli	262:290	Recently, smart hydrogels have attracted much attention for their abilities to respond to subtle changes in external and internal stimuli.					
34455316	0	7	with	polysaccharide	84:97	arg1	adhesiveness					113:124	enhanced adhesiveness	104:124	enhanced adhesiveness	104:124	Development of the mussel-inspired pH-responsive hydrogel based on Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties.					
34455316	0	7	with	polysaccharide	84:97	arg1	properties					142:151	antioxidant properties	130:151	antioxidant properties	130:151	Development of the mussel-inspired pH-responsive hydrogel based on Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties.					
34455316	2	8	theme	polysaccharide-based	307:326	arg1	biomaterials					328:339	natural polysaccharide-based biomaterials	299:339	natural polysaccharide-based biomaterials	299:339	Also, natural polysaccharide-based biomaterials are more appealing for their biocompatibility and biodegradability.					
34455316	0	9	theme	enhanced	104:111	arg1	adhesiveness					113:124	enhanced adhesiveness	104:124	enhanced adhesiveness	104:124	Development of the mussel-inspired pH-responsive hydrogel based on Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties.					
34455316	2	10	theme	natural	299:305	arg1	biomaterials					328:339	natural polysaccharide-based biomaterials	299:339	natural polysaccharide-based biomaterials	299:339	Also, natural polysaccharide-based biomaterials are more appealing for their biocompatibility and biodegradability.					
34455316	3	11	theme	smart	605:609	arg1	hydrogels					611:619	novel multifunctional smart hydrogels	583:619	novel multifunctional smart hydrogels	583:619	However, limitations owing to their complex compositions and mechanisms, cumbersome synthetic routes, and single function call for a simple and effective strategy to develop novel multifunctional smart hydrogels.					
34455316	0	12	theme	antioxidant	130:140	arg1	properties					142:151	antioxidant properties	130:151	antioxidant properties	130:151	Development of the mussel-inspired pH-responsive hydrogel based on Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties.					
34455316	4	13	with	glucomannan	729:739	arg1	bioactivities					754:766	diverse bioactivities	746:766	diverse bioactivities	746:766	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	13	with	glucomannan	729:739	arg1	biocompatibility					772:787	biocompatibility	772:787	biocompatibility	772:787	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	7	14	theme	antibacterial	1364:1376	arg1	activity					1378:1385	excellent and long-lasting antibacterial activity	1337:1385	excellent and long-lasting antibacterial activity	1337:1385	Meanwhile, the highly porous structure of hydrogel allowed berberine to be encapsulated and released to exhibit excellent and long-lasting antibacterial activity.					
34455316	6	15	theme	hydrogel	1215:1222	arg1	capacities					1197:1206	antioxidant capacities	1185:1206	antioxidant capacities	1185:1206	The presence of catechol groups greatly enhanced the tissue adhesion and antioxidant capacities of the hydrogel.					
34455316	6	15	theme	hydrogel	1215:1222	arg1	adhesion					1172:1179	tissue adhesion	1165:1179	tissue adhesion	1165:1179	The presence of catechol groups greatly enhanced the tissue adhesion and antioxidant capacities of the hydrogel.					
34455316	1	16	theme	much	195:198	arg1	attention					200:208	much attention	195:208	much attention	195:208	Recently, smart hydrogels have attracted much attention for their abilities to respond to subtle changes in external and internal stimuli.					
34455316	4	17	theme	CBSP	898:901	arg1	conjugate					917:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate	876:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties	876:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	6	18	theme	antioxidant	1185:1195	arg1	capacities					1197:1206	antioxidant capacities	1185:1206	antioxidant capacities	1185:1206	The presence of catechol groups greatly enhanced the tissue adhesion and antioxidant capacities of the hydrogel.					
34455316	3	19	theme	novel	583:587	arg1	hydrogels					611:619	novel multifunctional smart hydrogels	583:619	novel multifunctional smart hydrogels	583:619	However, limitations owing to their complex compositions and mechanisms, cumbersome synthetic routes, and single function call for a simple and effective strategy to develop novel multifunctional smart hydrogels.					
34455316	7	20	theme	hydrogel	1267:1274	arg1	structure					1254:1262	the highly porous structure	1236:1262	the highly porous structure of hydrogel	1236:1274	Meanwhile, the highly porous structure of hydrogel allowed berberine to be encapsulated and released to exhibit excellent and long-lasting antibacterial activity.					
34455316	7	20	theme	hydrogel	1267:1274	arg1	Meanwhile					1225:1233	Meanwhile	1225:1233	Meanwhile	1225:1233	Meanwhile, the highly porous structure of hydrogel allowed berberine to be encapsulated and released to exhibit excellent and long-lasting antibacterial activity.					
34455316	5	21	with	hydrogel	1082:1089	arg1	sensitivity					1099:1109	pH sensitivity	1096:1109	pH sensitivity	1096:1109	In this hydrogel system, CBSP as the backbone material, was negatively charged and conferred the hydrogel with pH sensitivity.					
34455316	4	22	theme	-dopamine	903:911	arg1	conjugate					917:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate	876:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties	876:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	23	theme	multifunctional	815:829	arg1	hydrogel					831:838	a low-cost multifunctional hydrogel	804:838	a low-cost multifunctional hydrogel	804:838	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	3	24	theme	cumbersome	482:491	arg1	routes					503:508	cumbersome synthetic routes	482:508	cumbersome synthetic routes	482:508	However, limitations owing to their complex compositions and mechanisms, cumbersome synthetic routes, and single function call for a simple and effective strategy to develop novel multifunctional smart hydrogels.					
34455316	0	25	theme	mussel-inspired	19:33	arg1	hydrogel					49:56	the mussel-inspired pH-responsive hydrogel	15:56	the mussel-inspired pH-responsive hydrogel	15:56	Development of the mussel-inspired pH-responsive hydrogel based on Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties.					
34455316	4	26	theme	pH-responsive	959:971	arg1	properties					973:982	pH-responsive properties	959:982	pH-responsive properties	959:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	8	27	theme	multifunctional	1459:1473	arg1	hydrogel					1475:1482	antibacterial multifunctional hydrogel	1445:1482	antibacterial multifunctional hydrogel	1445:1482	In summary, the adhesion, antioxidant, pH-sensitive, and antibacterial multifunctional hydrogel showed massive potential in the biomedical field.					
34455316	5	28	theme	hydrogel	993:1000	arg1	system					1002:1007	this hydrogel system	988:1007	this hydrogel system	988:1007	In this hydrogel system, CBSP as the backbone material, was negatively charged and conferred the hydrogel with pH sensitivity.					
34455316	8	29	theme	antibacterial	1445:1457	arg1	hydrogel					1475:1482	antibacterial multifunctional hydrogel	1445:1482	antibacterial multifunctional hydrogel	1445:1482	In summary, the adhesion, antioxidant, pH-sensitive, and antibacterial multifunctional hydrogel showed massive potential in the biomedical field.					
34455316	3	30	theme	single	515:520	arg1	function					522:529	single function	515:529	single function	515:529	However, limitations owing to their complex compositions and mechanisms, cumbersome synthetic routes, and single function call for a simple and effective strategy to develop novel multifunctional smart hydrogels.					
34455316	8	31	theme	biomedical	1516:1525	arg1	field					1527:1531	the biomedical field	1512:1531	the biomedical field	1512:1531	In summary, the adhesion, antioxidant, pH-sensitive, and antibacterial multifunctional hydrogel showed massive potential in the biomedical field.					
34455316	4	32	theme	glucomannan	729:739	arg1	polysaccharide					689:702	Bletilla striata polysaccharide	672:702	Bletilla striata polysaccharide (BSP)	672:708	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	32	theme	glucomannan	729:739	arg1	kind					713:716	a kind	711:716	a kind of natural glucomannan with diverse bioactivities and biocompatibility	711:787	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	33	theme	striata	681:687	arg1	BSP					705:707	BSP	705:707	BSP	705:707	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	33	theme	striata	681:687	arg1	polysaccharide					689:702	Bletilla striata polysaccharide	672:702	Bletilla striata polysaccharide (BSP)	672:708	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	33	theme	striata	681:687	arg1	kind					713:716	a kind	711:716	a kind of natural glucomannan with diverse bioactivities and biocompatibility	711:787	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	0	34	theme	hydrogel	49:56	arg1	Development					0:10	Development	0:10	Development of the mussel-inspired pH-responsive hydrogel	0:56	Development of the mussel-inspired pH-responsive hydrogel based on Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties.					
34455316	4	35	theme	diverse	746:752	arg1	bioactivities					754:766	diverse bioactivities	746:766	diverse bioactivities	746:766	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	6	36	theme	tissue	1165:1170	arg1	adhesion					1172:1179	tissue adhesion	1165:1179	tissue adhesion	1165:1179	The presence of catechol groups greatly enhanced the tissue adhesion and antioxidant capacities of the hydrogel.					
34455316	4	37	theme	carboxymethylated	876:892	arg1	conjugate					917:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate	876:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties	876:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	5	38	from	charged	1056:1062	arg1	system					1002:1007	this hydrogel system	988:1007	this hydrogel system	988:1007	In this hydrogel system, CBSP as the backbone material, was negatively charged and conferred the hydrogel with pH sensitivity.					
34455316	7	39	theme	excellent	1337:1345	arg1	activity					1378:1385	excellent and long-lasting antibacterial activity	1337:1385	excellent and long-lasting antibacterial activity	1337:1385	Meanwhile, the highly porous structure of hydrogel allowed berberine to be encapsulated and released to exhibit excellent and long-lasting antibacterial activity.					
34455316	0	40	theme	pH-responsive	35:47	arg1	hydrogel					49:56	the mussel-inspired pH-responsive hydrogel	15:56	the mussel-inspired pH-responsive hydrogel	15:56	Development of the mussel-inspired pH-responsive hydrogel based on Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties.					
34455316	4	41	theme	low-cost	806:813	arg1	hydrogel					831:838	a low-cost multifunctional hydrogel	804:838	a low-cost multifunctional hydrogel	804:838	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	3	42	theme	complex	445:451	arg1	compositions					453:464	their complex compositions	439:464	their complex compositions	439:464	However, limitations owing to their complex compositions and mechanisms, cumbersome synthetic routes, and single function call for a simple and effective strategy to develop novel multifunctional smart hydrogels.					
34455316	3	43	theme	synthetic	493:501	arg1	routes					503:508	cumbersome synthetic routes	482:508	cumbersome synthetic routes	482:508	However, limitations owing to their complex compositions and mechanisms, cumbersome synthetic routes, and single function call for a simple and effective strategy to develop novel multifunctional smart hydrogels.					
34455316	4	44	theme	BSP	894:896	arg1	conjugate					917:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate	876:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties	876:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	7	45	theme	porous	1247:1252	arg1	structure					1254:1262	the highly porous structure	1236:1262	the highly porous structure of hydrogel	1236:1274	Meanwhile, the highly porous structure of hydrogel allowed berberine to be encapsulated and released to exhibit excellent and long-lasting antibacterial activity.					
34455316	7	45	theme	porous	1247:1252	arg1	Meanwhile					1225:1233	Meanwhile	1225:1233	Meanwhile	1225:1233	Meanwhile, the highly porous structure of hydrogel allowed berberine to be encapsulated and released to exhibit excellent and long-lasting antibacterial activity.					
34455316	3	46	theme	simple	542:547	arg1	strategy					563:570	a simple and effective strategy	540:570	a simple and effective strategy to develop novel multifunctional smart hydrogels	540:619	However, limitations owing to their complex compositions and mechanisms, cumbersome synthetic routes, and single function call for a simple and effective strategy to develop novel multifunctional smart hydrogels.					
34455316	8	47	theme	massive	1491:1497	arg1	potential					1499:1507	massive potential	1491:1507	massive potential	1491:1507	In summary, the adhesion, antioxidant, pH-sensitive, and antibacterial multifunctional hydrogel showed massive potential in the biomedical field.					
34455316	5	48	theme	backbone	1022:1029	arg1	material					1031:1038	the backbone material	1018:1038	the backbone material	1018:1038	In this hydrogel system, CBSP as the backbone material, was negatively charged and conferred the hydrogel with pH sensitivity.					
34455316	4	49	with	conjugate	917:925	arg1	antioxidant					942:952	antioxidant	942:952	antioxidant	942:952	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	49	with	conjugate	917:925	arg1	adhesion					932:939	adhesion	932:939	adhesion	932:939	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	49	with	conjugate	917:925	arg1	properties					973:982	pH-responsive properties	959:982	pH-responsive properties	959:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	1	50	theme	subtle	244:249	arg1	changes					251:257	subtle changes	244:257	subtle changes in external and internal stimuli	244:290	Recently, smart hydrogels have attracted much attention for their abilities to respond to subtle changes in external and internal stimuli.					
34455316	1	51	from	changes	251:257	arg1	stimuli					284:290	external and internal stimuli	262:290	external and internal stimuli	262:290	Recently, smart hydrogels have attracted much attention for their abilities to respond to subtle changes in external and internal stimuli.					
34455316	3	52	theme	multifunctional	589:603	arg1	hydrogels					611:619	novel multifunctional smart hydrogels	583:619	novel multifunctional smart hydrogels	583:619	However, limitations owing to their complex compositions and mechanisms, cumbersome synthetic routes, and single function call for a simple and effective strategy to develop novel multifunctional smart hydrogels.					
34455316	4	53	theme	conjugate	917:925	arg1	groups					866:871	the catechol groups	853:871	the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties	853:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	53	theme	conjugate	917:925	arg1	conjugate					917:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate	876:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties	876:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	0	54	theme	striata	76:82	arg1	polysaccharide					84:97	Bletilla striata polysaccharide	67:97	Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties	67:151	Development of the mussel-inspired pH-responsive hydrogel based on Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties.					
34455316	4	55	dep	achieved	654:661	arg1	fabricated					793:802	fabricated	793:802	fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties	793:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	6	56	dep	adhesion	1172:1179	arg1	the					1161:1163	the	1161:1163	the	1161:1163	The presence of catechol groups greatly enhanced the tissue adhesion and antioxidant capacities of the hydrogel.					
34455316	7	57	theme	long-lasting	1351:1362	arg1	activity					1378:1385	excellent and long-lasting antibacterial activity	1337:1385	excellent and long-lasting antibacterial activity	1337:1385	Meanwhile, the highly porous structure of hydrogel allowed berberine to be encapsulated and released to exhibit excellent and long-lasting antibacterial activity.					
34455316	1	58	theme	smart	164:168	arg1	hydrogels					170:178	smart hydrogels	164:178	smart hydrogels	164:178	Recently, smart hydrogels have attracted much attention for their abilities to respond to subtle changes in external and internal stimuli.					
34455316	5	59	from	system	1002:1007	arg1	charged					1056:1062	charged	1056:1062	charged	1056:1062	In this hydrogel system, CBSP as the backbone material, was negatively charged and conferred the hydrogel with pH sensitivity.					
34455316	4	60	theme	Bletilla	672:679	arg1	BSP					705:707	BSP	705:707	BSP	705:707	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	60	theme	Bletilla	672:679	arg1	polysaccharide					689:702	Bletilla striata polysaccharide	672:702	Bletilla striata polysaccharide (BSP)	672:708	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	60	theme	Bletilla	672:679	arg1	kind					713:716	a kind	711:716	a kind of natural glucomannan with diverse bioactivities and biocompatibility	711:787	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	0	61	theme	Bletilla	67:74	arg1	polysaccharide					84:97	Bletilla striata polysaccharide	67:97	Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties	67:151	Development of the mussel-inspired pH-responsive hydrogel based on Bletilla striata polysaccharide with enhanced adhesiveness and antioxidant properties.					
34455316	4	62	theme	catechol	857:864	arg1	groups					866:871	the catechol groups	853:871	the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties	853:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	4	62	theme	catechol	857:864	arg1	conjugate					917:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate	876:925	carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties	876:982	Herein, this developed work was achieved based on Bletilla striata polysaccharide (BSP), a kind of natural glucomannan with diverse bioactivities and biocompatibility, we fabricated a low-cost multifunctional hydrogel by oxidizing the catechol groups of carboxymethylated BSP(CBSP)-dopamine(DA) conjugate with adhesion, antioxidant, and pH-responsive properties.					
34455316	6	63	theme	groups	1137:1142	arg1	presence					1116:1123	The presence	1112:1123	The presence of catechol groups	1112:1142	The presence of catechol groups greatly enhanced the tissue adhesion and antioxidant capacities of the hydrogel.					
34455316	1	64	theme	external	262:269	arg1	stimuli					284:290	external and internal stimuli	262:290	external and internal stimuli	262:290	Recently, smart hydrogels have attracted much attention for their abilities to respond to subtle changes in external and internal stimuli.					
34740051	0	0	theme	microfibrillated	91:106	arg1	cellulose					108:116	microfibrillated cellulose	91:116	microfibrillated cellulose	91:116	A comparative investigation of gelatinized and regenerated starch composites reinforced by microfibrillated cellulose.					
34740051	1	1	theme	GS	356:357	arg1	films					360:364	traditional gelatinized starch (GS) films	324:364	traditional gelatinized starch (GS) films	324:364	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	2	2	dep	0 °C.	450:454	arg1	prepared					520:527	prepared	520:527	were prepared by dialyzing and solution-casting method	515:568	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	2	3	theme	Pure	367:370	arg1	starch					372:377	Pure starch	367:377	Pure starch	367:377	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	3	4	dep	increased	642:650	arg1	compared					672:679	compared	672:679	compared with that of GS film, respectively	672:714	Results showed that the tensile strength and elongation at break of RS increased by 44.8% and 82.4%, compared with that of GS film, respectively.					
34740051	2	5	theme	%	401:401	arg1	solution					438:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution	396:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method	396:568	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	4	6	theme	lower	817:821	arg1	crystallinity					823:835	lower crystallinity	817:835	lower crystallinity	817:835	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	4	7	theme	improved	916:923	arg1	properties					936:945	significantly improved mechanical properties	902:945	significantly improved mechanical properties	902:945	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	4	8	theme	smaller	841:847	arg1	size					863:866	smaller crystal grain size	841:866	smaller crystal grain size	841:866	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	3	9	dep	strength	603:610	arg1	the					591:593	the	591:593	the	591:593	Results showed that the tensile strength and elongation at break of RS increased by 44.8% and 82.4%, compared with that of GS film, respectively.					
34740051	3	10	from	break	630:634	arg1	strength					603:610	tensile strength	595:610	tensile strength	595:610	Results showed that the tensile strength and elongation at break of RS increased by 44.8% and 82.4%, compared with that of GS film, respectively.					
34740051	3	10	from	break	630:634	arg1	elongation					616:625	elongation	616:625	elongation	616:625	Results showed that the tensile strength and elongation at break of RS increased by 44.8% and 82.4%, compared with that of GS film, respectively.					
34740051	2	11	from	0 °C.	450:454	arg1	solution					438:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution	396:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method	396:568	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	1	12	theme	1.4-fold	278:285	arg1	improvement					287:297	a nearly 1.4-fold improvement	269:297	a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films	269:364	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	4	13	theme	amylose	800:806	arg1	content					808:814	higher amylose content	793:814	higher amylose content	793:814	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	2	14	theme	sodium	414:419	arg1	solution					438:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution	396:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method	396:568	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	5	15	theme	novel	952:956	arg1	helpful					994:1000	helpful	994:1000	helpful	994:1000	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	5	15	theme	novel	952:956	arg1	strategy					958:965	The novel strategy	948:965	The novel strategy used in this study	948:984	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	5	16	with	materials	1034:1042	arg1	biodegradability					1085:1100	biodegradability	1085:1100	biodegradability	1085:1100	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	5	16	with	materials	1034:1042	arg1	properties					1070:1079	excellent mechanical properties	1049:1079	excellent mechanical properties	1049:1079	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	1	17	theme	regenerated	191:201	arg1	RS					211:212	RS	211:212	RS	211:212	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	1	17	theme	regenerated	191:201	arg1	starch					203:208	regenerated starch	191:208	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	2	18	theme	biocomposite	481:492	arg1	films					494:498	their biocomposite films	475:498	their biocomposite films	475:498	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	4	19	theme	higher	793:798	arg1	content					808:814	higher amylose content	793:814	higher amylose content	793:814	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	1	20	theme	starch	203:208	arg1	films					258:262	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	0	21	theme	comparative	2:12	arg1	investigation					14:26	A comparative investigation	0:26	A comparative investigation of gelatinized and regenerated starch composites	0:75	A comparative investigation of gelatinized and regenerated starch composites reinforced by microfibrillated cellulose.					
34740051	2	22	theme	RS	462:463	arg1	films					465:469	RS films	462:469	RS films	462:469	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	1	23	from	improvement	287:297	arg1	strength					310:317	tensile strength	302:317	tensile strength	302:317	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	5	24	theme	sustainable	1167:1177	arg1	materials					1179:1187	sustainable materials	1167:1187	sustainable materials	1167:1187	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	5	25	theme	materials	1179:1187	arg1	development					1152:1162	the development	1148:1162	the development of sustainable materials	1148:1187	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	3	26	theme	RS	639:640	arg1	strength					603:610	tensile strength	595:610	tensile strength	595:610	Results showed that the tensile strength and elongation at break of RS increased by 44.8% and 82.4%, compared with that of GS film, respectively.					
34740051	3	26	theme	RS	639:640	arg1	elongation					616:625	elongation	616:625	elongation	616:625	Results showed that the tensile strength and elongation at break of RS increased by 44.8% and 82.4%, compared with that of GS film, respectively.					
34740051	2	27	theme	NaOH	432:435	arg1	solution					438:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution	396:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method	396:568	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	4	28	theme	composite	876:884	arg1	films					886:890	RS/MFC composite films	869:890	RS/MFC composite films	869:890	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	2	29	theme	hydroxide	421:429	arg1	solution					438:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution	396:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method	396:568	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	1	30	theme	tensile	302:308	arg1	strength					310:317	tensile strength	302:317	tensile strength	302:317	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	4	31	theme	RS/MFC	869:874	arg1	films					886:890	RS/MFC composite films	869:890	RS/MFC composite films	869:890	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	1	32	theme	/microfibrillated	214:230	arg1	films					258:262	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	5	33	theme	petrochemical	1121:1133	arg1	plastics					1135:1142	petrochemical plastics	1121:1142	petrochemical plastics for the development of sustainable materials	1121:1187	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	2	34	theme	%	412:412	arg1	solution					438:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution	396:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method	396:568	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	4	35	theme	molecular	775:783	arg1	weight					785:790	viscosity-average molecular weight	757:790	viscosity-average molecular weight	757:790	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	1	36	theme	cellulose	232:240	arg1	films					258:262	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	0	37	theme	gelatinized	31:41	arg1	composites					66:75	gelatinized and regenerated starch composites	31:75	gelatinized and regenerated starch composites	31:75	A comparative investigation of gelatinized and regenerated starch composites reinforced by microfibrillated cellulose.					
34740051	5	38	theme	mechanical	1059:1068	arg1	properties					1070:1079	excellent mechanical properties	1049:1079	excellent mechanical properties	1049:1079	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	4	39	theme	viscosity-average	757:773	arg1	weight					785:790	viscosity-average molecular weight	757:790	viscosity-average molecular weight	757:790	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	2	40	theme	urea/4 wt	403:411	arg1	solution					438:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution	396:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method	396:568	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	0	41	theme	starch	59:64	arg1	composites					66:75	gelatinized and regenerated starch composites	31:75	gelatinized and regenerated starch composites	31:75	A comparative investigation of gelatinized and regenerated starch composites reinforced by microfibrillated cellulose.					
34740051	1	42	theme	traditional	324:334	arg1	films					360:364	traditional gelatinized starch (GS) films	324:364	traditional gelatinized starch (GS) films	324:364	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	4	43	theme	grain	857:861	arg1	size					863:866	smaller crystal grain size	841:866	smaller crystal grain size	841:866	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	0	44	theme	regenerated	47:57	arg1	composites					66:75	gelatinized and regenerated starch composites	31:75	gelatinized and regenerated starch composites	31:75	A comparative investigation of gelatinized and regenerated starch composites reinforced by microfibrillated cellulose.					
34740051	1	45	theme	gelatinized	336:346	arg1	films					360:364	traditional gelatinized starch (GS) films	324:364	traditional gelatinized starch (GS) films	324:364	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	4	46	theme	crystal	849:855	arg1	size					863:866	smaller crystal grain size	841:866	smaller crystal grain size	841:866	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	1	47	theme	starch	348:353	arg1	films					360:364	traditional gelatinized starch (GS) films	324:364	traditional gelatinized starch (GS) films	324:364	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	2	48	theme	solution-casting	546:561	arg1	method					563:568	and solution-casting method	542:568	method	563:568	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	5	49	theme	regenerated	1015:1025	arg1	materials					1034:1042	regenerated starch materials	1015:1042	regenerated starch materials with excellent mechanical properties and biodegradability	1015:1100	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	5	49	theme	regenerated	1015:1025	arg1	alternatives					1105:1116	alternatives	1105:1116	alternatives to petrochemical plastics for the development of sustainable materials	1105:1187	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	4	50	dep	lower	751:755	arg1	size					863:866	smaller crystal grain size	841:866	smaller crystal grain size	841:866	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	4	50	dep	lower	751:755	arg1	crystallinity					823:835	lower crystallinity	817:835	lower crystallinity	817:835	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	4	50	dep	lower	751:755	arg1	weight					785:790	viscosity-average molecular weight	757:790	viscosity-average molecular weight	757:790	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	4	50	dep	lower	751:755	arg1	content					808:814	higher amylose content	793:814	higher amylose content	793:814	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	1	51	theme	novel	148:152	arg1	method					170:175	a novel and ecofriendly method	146:175	a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films	146:364	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	1	52	theme	MFC	243:245	arg1	films					258:262	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	5	53	theme	starch	1027:1032	arg1	materials					1034:1042	regenerated starch materials	1015:1042	regenerated starch materials with excellent mechanical properties and biodegradability	1015:1100	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	5	53	theme	starch	1027:1032	arg1	alternatives					1105:1116	alternatives	1105:1116	alternatives to petrochemical plastics for the development of sustainable materials	1105:1187	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	4	54	theme	adequate	730:737	arg1	lower					751:755	lower	751:755	lower	751:755	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	4	54	theme	adequate	730:737	arg1	dispersion					739:748	the adequate dispersion	726:748	the adequate dispersion	726:748	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
34740051	5	55	theme	excellent	1049:1057	arg1	properties					1070:1079	excellent mechanical properties	1049:1079	excellent mechanical properties	1049:1079	The novel strategy used in this study will be helpful in preparing regenerated starch materials with excellent mechanical properties and biodegradability as alternatives to petrochemical plastics for the development of sustainable materials.					
34740051	3	56	theme	GS	694:695	arg1	film					697:700	GS film	694:700	GS film	694:700	Results showed that the tensile strength and elongation at break of RS increased by 44.8% and 82.4%, compared with that of GS film, respectively.					
34740051	3	57	theme	tensile	595:601	arg1	strength					603:610	tensile strength	595:610	tensile strength	595:610	Results showed that the tensile strength and elongation at break of RS increased by 44.8% and 82.4%, compared with that of GS film, respectively.					
34740051	1	58	theme	ecofriendly	158:168	arg1	method					170:175	a novel and ecofriendly method	146:175	a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films	146:364	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	2	59	theme	14 wt	396:400	arg1	solution					438:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution	396:445	14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method	396:568	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	1	60	theme	composite	248:256	arg1	films					258:262	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	regenerated starch (RS)/microfibrillated cellulose (MFC) composite films	191:262	This research demonstrated a novel and ecofriendly method for producing regenerated starch (RS)/microfibrillated cellulose (MFC) composite films with a nearly 1.4-fold improvement in tensile strength than traditional gelatinized starch (GS) films.					
34740051	0	61	theme	composites	66:75	arg1	investigation					14:26	A comparative investigation	0:26	A comparative investigation of gelatinized and regenerated starch composites	0:75	A comparative investigation of gelatinized and regenerated starch composites reinforced by microfibrillated cellulose.					
34740051	2	62	contain	containing	500:509	arg2	MFC					511:513	MFC	511:513	MFC	511:513	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	2	62	contain	containing	500:509	arg1	films					465:469	RS films	462:469	RS films	462:469	Pure starch was dissolved in 14 wt% urea/4 wt% sodium hydroxide (NaOH) solution at 0 °C. Then, RS films and their biocomposite films containing MFC were prepared by dialyzing and solution-casting method.					
34740051	4	63	theme	mechanical	925:934	arg1	properties					936:945	significantly improved mechanical properties	902:945	significantly improved mechanical properties	902:945	Owing to the adequate dispersion, lower viscosity-average molecular weight, higher amylose content, lower crystallinity and smaller crystal grain size, RS/MFC composite films exhibited significantly improved mechanical properties.					
32961189	1	0	dep	polysaccharides	102:116	arg1	PCPS2					135:139	PCPS2	135:139	PCPS2	135:139	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	1	0	dep	polysaccharides	102:116	arg1	PCPS1					125:129	PCPS1	125:129	PCPS1	125:129	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	1	0	dep	polysaccharides	102:116	arg1	PCPW					119:122	PCPW	119:122	PCPW	119:122	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	1	0	dep	polysaccharides	102:116	arg1	polysaccharides					102:116	Three novel polysaccharides	90:116	Three novel polysaccharides (PCPW, PCPS1 and PCPS2)	90:140	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	7	1	theme	great	1127:1131	arg1	potential					1133:1141	great potential	1127:1141	great potential	1127:1141	Overall, three polysaccharides showed a good immunological activity and pose great potential as a novel food or drug additive.					
32961189	7	1	theme	great	1127:1131	arg1	food					1154:1157	a novel food	1146:1157	a novel food	1146:1157	Overall, three polysaccharides showed a good immunological activity and pose great potential as a novel food or drug additive.					
32961189	4	2	from	1	778:778	arg1	addition					716:723	addition	716:723	addition	716:723	In addition, the main linkages of glycosidic bonds of PCPS2 were 1, 4-linked-rha, 1, 4-linked-man,1, 4-linked-galA and 1, 6-linked-man.					
32961189	0	3	from	chinensis	79:87	arg1	polysaccharides					47:61	polysaccharides	47:61	polysaccharides from Potentilla chinensis	47:87	Characterization and immunological activity of polysaccharides from Potentilla chinensis.					
32961189	0	3	from	chinensis	79:87	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and immunological activity of polysaccharides from Potentilla chinensis.					
32961189	0	3	from	chinensis	79:87	arg1	activity					35:42	immunological activity	21:42	immunological activity	21:42	Characterization and immunological activity of polysaccharides from Potentilla chinensis.					
32961189	5	4	theme	splenocyte	964:973	arg1	proliferation					975:987	splenocyte proliferation	964:987	splenocyte proliferation	964:987	Immunological tests indicated that both PCPW and PCPS2 could increase NO production of RAW264.7 cells, and promote splenocyte proliferation.					
32961189	0	5	from	Characterization	0:15	arg1	chinensis					79:87	chinensis	79:87	chinensis	79:87	Characterization and immunological activity of polysaccharides from Potentilla chinensis.					
32961189	2	6	theme	PCPS1	297:301	arg1	4.45 × 103 Da					335:347	4.45 × 103 Da	335:347	4.45 × 103 Da	335:347	The molecular weights of PCPW, PCPS1 and PCPS2 were calculated to be 4.45 × 103 Da, 1.18 × 104 Da and 4.23 × 104 Da, respectively.					
32961189	2	6	theme	PCPS1	297:301	arg1	weights					280:286	The molecular weights	266:286	The molecular weights of PCPW, PCPS1 and PCPS2	266:311	The molecular weights of PCPW, PCPS1 and PCPS2 were calculated to be 4.45 × 103 Da, 1.18 × 104 Da and 4.23 × 104 Da, respectively.					
32961189	5	7	theme	cells	945:949	arg1	production					922:931	NO production	919:931	NO production of RAW264.7 cells	919:949	Immunological tests indicated that both PCPW and PCPS2 could increase NO production of RAW264.7 cells, and promote splenocyte proliferation.					
32961189	1	8	attach	isolated	147:154	arg2	PCPS1					125:129	PCPS1	125:129	PCPS1	125:129	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	1	8	attach	isolated	147:154	arg1	chinensis					172:180	chinensis	172:180	chinensis	172:180	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	1	8	attach	isolated	147:154	arg2	PCPW					119:122	PCPW	119:122	PCPW	119:122	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	1	8	attach	isolated	147:154	arg2	polysaccharides					102:116	Three novel polysaccharides	90:116	Three novel polysaccharides (PCPW, PCPS1 and PCPS2)	90:140	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	1	8	attach	isolated	147:154	arg2	PCPS2					135:139	PCPS2	135:139	PCPS2	135:139	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	7	9	theme	good	1090:1093	arg1	activity					1109:1116	a good immunological activity	1088:1116	a good immunological activity	1088:1116	Overall, three polysaccharides showed a good immunological activity and pose great potential as a novel food or drug additive.					
32961189	2	10	theme	PCPW	291:294	arg1	4.45 × 103 Da					335:347	4.45 × 103 Da	335:347	4.45 × 103 Da	335:347	The molecular weights of PCPW, PCPS1 and PCPS2 were calculated to be 4.45 × 103 Da, 1.18 × 104 Da and 4.23 × 104 Da, respectively.					
32961189	2	10	theme	PCPW	291:294	arg1	weights					280:286	The molecular weights	266:286	The molecular weights of PCPW, PCPS1 and PCPS2	266:311	The molecular weights of PCPW, PCPS1 and PCPS2 were calculated to be 4.45 × 103 Da, 1.18 × 104 Da and 4.23 × 104 Da, respectively.					
32961189	7	11	theme	immunological	1095:1107	arg1	activity					1109:1116	a good immunological activity	1088:1116	a good immunological activity	1088:1116	Overall, three polysaccharides showed a good immunological activity and pose great potential as a novel food or drug additive.					
32961189	1	12	theme	novel	96:100	arg1	PCPS2					135:139	PCPS2	135:139	PCPS2	135:139	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	1	12	theme	novel	96:100	arg1	PCPS1					125:129	PCPS1	125:129	PCPS1	125:129	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	1	12	theme	novel	96:100	arg1	PCPW					119:122	PCPW	119:122	PCPW	119:122	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	1	12	theme	novel	96:100	arg1	polysaccharides					102:116	Three novel polysaccharides	90:116	Three novel polysaccharides (PCPW, PCPS1 and PCPS2)	90:140	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	5	13	theme	Immunological	849:861	arg1	tests					863:867	Immunological tests	849:867	Immunological tests	849:867	Immunological tests indicated that both PCPW and PCPS2 could increase NO production of RAW264.7 cells, and promote splenocyte proliferation.					
32961189	5	14	theme	RAW264.7	936:943	arg1	cells					945:949	RAW264.7 cells	936:949	RAW264.7 cells	936:949	Immunological tests indicated that both PCPW and PCPS2 could increase NO production of RAW264.7 cells, and promote splenocyte proliferation.					
32961189	7	15	theme	additive	1167:1174	arg1	potential					1133:1141	great potential	1127:1141	great potential	1127:1141	Overall, three polysaccharides showed a good immunological activity and pose great potential as a novel food or drug additive.					
32961189	7	15	theme	additive	1167:1174	arg1	food					1154:1157	a novel food	1146:1157	a novel food	1146:1157	Overall, three polysaccharides showed a good immunological activity and pose great potential as a novel food or drug additive.					
32961189	4	16	theme	PCPS2	767:771	arg1	bonds					758:762	glycosidic bonds	747:762	glycosidic bonds of PCPS2	747:771	In addition, the main linkages of glycosidic bonds of PCPS2 were 1, 4-linked-rha, 1, 4-linked-man,1, 4-linked-galA and 1, 6-linked-man.					
32961189	4	17	theme	bonds	758:762	arg1	linkages					735:742	the main linkages	726:742	the main linkages of glycosidic bonds of PCPS2	726:771	In addition, the main linkages of glycosidic bonds of PCPS2 were 1, 4-linked-rha, 1, 4-linked-man,1, 4-linked-galA and 1, 6-linked-man.					
32961189	4	17	theme	bonds	758:762	arg1	1					778:778	1	778:778	1	778:778	In addition, the main linkages of glycosidic bonds of PCPS2 were 1, 4-linked-rha, 1, 4-linked-man,1, 4-linked-galA and 1, 6-linked-man.					
32961189	1	18	theme	structural	199:208	arg1	analysis					210:217	structural analysis	199:217	structural analysis	199:217	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	0	19	theme	immunological	21:33	arg1	activity					35:42	immunological activity	21:42	immunological activity	21:42	Characterization and immunological activity of polysaccharides from Potentilla chinensis.					
32961189	4	20	theme	glycosidic	747:756	arg1	bonds					758:762	glycosidic bonds	747:762	glycosidic bonds of PCPS2	747:771	In addition, the main linkages of glycosidic bonds of PCPS2 were 1, 4-linked-rha, 1, 4-linked-man,1, 4-linked-galA and 1, 6-linked-man.					
32961189	6	21	theme	NF-κB	1043:1047	arg1	activators					1029:1038	activators	1029:1038	activators of NF-κB	1029:1047	All three polysaccharides proved to be activators of NF-κB.					
32961189	0	22	theme	polysaccharides	47:61	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and immunological activity of polysaccharides from Potentilla chinensis.					
32961189	0	22	theme	polysaccharides	47:61	arg1	activity					35:42	immunological activity	21:42	immunological activity	21:42	Characterization and immunological activity of polysaccharides from Potentilla chinensis.					
32961189	7	23	theme	novel	1148:1152	arg1	potential					1133:1141	great potential	1127:1141	great potential	1127:1141	Overall, three polysaccharides showed a good immunological activity and pose great potential as a novel food or drug additive.					
32961189	7	23	theme	novel	1148:1152	arg1	food					1154:1157	a novel food	1146:1157	a novel food	1146:1157	Overall, three polysaccharides showed a good immunological activity and pose great potential as a novel food or drug additive.					
32961189	3	24	theme	monosaccharides	409:423	arg1	composition					425:435	monosaccharides composition	409:435	monosaccharides composition	409:435	Analysis of monosaccharides composition confirmed that PCPW was composed of mannose, rhamnose, glucose, galactose and arabinose, while the two acidic polysaccharides PCPS1 and PCPS2 were consisted of six monosaccharides, including mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose respectively.					
32961189	3	25	theme	galacturonic	647:658	arg1	acid					660:663	galacturonic acid	647:663	galacturonic acid	647:663	Analysis of monosaccharides composition confirmed that PCPW was composed of mannose, rhamnose, glucose, galactose and arabinose, while the two acidic polysaccharides PCPS1 and PCPS2 were consisted of six monosaccharides, including mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose respectively.					
32961189	3	26	theme	composition	425:435	arg1	Analysis					397:404	Analysis	397:404	Analysis of monosaccharides composition	397:435	Analysis of monosaccharides composition confirmed that PCPW was composed of mannose, rhamnose, glucose, galactose and arabinose, while the two acidic polysaccharides PCPS1 and PCPS2 were consisted of six monosaccharides, including mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose respectively.					
32961189	3	27	dep	polysaccharides	547:561	arg1	polysaccharides					547:561	the two acidic polysaccharides	532:561	the two acidic polysaccharides PCPS1 and PCPS2	532:577	Analysis of monosaccharides composition confirmed that PCPW was composed of mannose, rhamnose, glucose, galactose and arabinose, while the two acidic polysaccharides PCPS1 and PCPS2 were consisted of six monosaccharides, including mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose respectively.					
32961189	3	27	dep	polysaccharides	547:561	arg1	PCPS2					573:577	PCPS2	573:577	PCPS2	573:577	Analysis of monosaccharides composition confirmed that PCPW was composed of mannose, rhamnose, glucose, galactose and arabinose, while the two acidic polysaccharides PCPS1 and PCPS2 were consisted of six monosaccharides, including mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose respectively.					
32961189	3	27	dep	polysaccharides	547:561	arg1	PCPS1					563:567	PCPS1	563:567	PCPS1	563:567	Analysis of monosaccharides composition confirmed that PCPW was composed of mannose, rhamnose, glucose, galactose and arabinose, while the two acidic polysaccharides PCPS1 and PCPS2 were consisted of six monosaccharides, including mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose respectively.					
32961189	2	28	theme	PCPS2	307:311	arg1	4.45 × 103 Da					335:347	4.45 × 103 Da	335:347	4.45 × 103 Da	335:347	The molecular weights of PCPW, PCPS1 and PCPS2 were calculated to be 4.45 × 103 Da, 1.18 × 104 Da and 4.23 × 104 Da, respectively.					
32961189	2	28	theme	PCPS2	307:311	arg1	weights					280:286	The molecular weights	266:286	The molecular weights of PCPW, PCPS1 and PCPS2	266:311	The molecular weights of PCPW, PCPS1 and PCPS2 were calculated to be 4.45 × 103 Da, 1.18 × 104 Da and 4.23 × 104 Da, respectively.					
32961189	1	29	theme	spectral	228:235	arg1	methods					257:263	spectral and physicochemical methods	228:263	spectral and physicochemical methods	228:263	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	3	30	theme	acidic	540:545	arg1	polysaccharides					547:561	the two acidic polysaccharides	532:561	the two acidic polysaccharides PCPS1 and PCPS2	532:577	Analysis of monosaccharides composition confirmed that PCPW was composed of mannose, rhamnose, glucose, galactose and arabinose, while the two acidic polysaccharides PCPS1 and PCPS2 were consisted of six monosaccharides, including mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose respectively.					
32961189	3	30	theme	acidic	540:545	arg1	PCPS2					573:577	PCPS2	573:577	PCPS2	573:577	Analysis of monosaccharides composition confirmed that PCPW was composed of mannose, rhamnose, glucose, galactose and arabinose, while the two acidic polysaccharides PCPS1 and PCPS2 were consisted of six monosaccharides, including mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose respectively.					
32961189	3	30	theme	acidic	540:545	arg1	PCPS1					563:567	PCPS1	563:567	PCPS1	563:567	Analysis of monosaccharides composition confirmed that PCPW was composed of mannose, rhamnose, glucose, galactose and arabinose, while the two acidic polysaccharides PCPS1 and PCPS2 were consisted of six monosaccharides, including mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose respectively.					
32961189	2	31	theme	molecular	270:278	arg1	4.45 × 103 Da					335:347	4.45 × 103 Da	335:347	4.45 × 103 Da	335:347	The molecular weights of PCPW, PCPS1 and PCPS2 were calculated to be 4.45 × 103 Da, 1.18 × 104 Da and 4.23 × 104 Da, respectively.					
32961189	2	31	theme	molecular	270:278	arg1	weights					280:286	The molecular weights	266:286	The molecular weights of PCPW, PCPS1 and PCPS2	266:311	The molecular weights of PCPW, PCPS1 and PCPS2 were calculated to be 4.45 × 103 Da, 1.18 × 104 Da and 4.23 × 104 Da, respectively.					
32961189	5	32	theme	NO	919:920	arg1	production					922:931	NO production	919:931	NO production of RAW264.7 cells	919:949	Immunological tests indicated that both PCPW and PCPS2 could increase NO production of RAW264.7 cells, and promote splenocyte proliferation.					
32961189	4	33	theme	main	730:733	arg1	linkages					735:742	the main linkages	726:742	the main linkages of glycosidic bonds of PCPS2	726:771	In addition, the main linkages of glycosidic bonds of PCPS2 were 1, 4-linked-rha, 1, 4-linked-man,1, 4-linked-galA and 1, 6-linked-man.					
32961189	4	33	theme	main	730:733	arg1	1					778:778	1	778:778	1	778:778	In addition, the main linkages of glycosidic bonds of PCPS2 were 1, 4-linked-rha, 1, 4-linked-man,1, 4-linked-galA and 1, 6-linked-man.					
32961189	1	34	theme	physicochemical	241:255	arg1	methods					257:263	spectral and physicochemical methods	228:263	spectral and physicochemical methods	228:263	Three novel polysaccharides (PCPW, PCPS1 and PCPS2) were isolated from Potentilla chinensis and subjected to structural analysis by using spectral and physicochemical methods.					
32961189	0	35	from	activity	35:42	arg1	chinensis					79:87	chinensis	79:87	chinensis	79:87	Characterization and immunological activity of polysaccharides from Potentilla chinensis.					
32835799	6	0	theme	repeating	862:870	arg1	-α1→					983:986	-α1→	983:986	-α1→	983:986	As a result, the repeating sequences of FCS and SFII were →4)-[L-Fuc3S4S-(α1 → 3)]-D-GlcA-(β1 → 3)-D-GalNAc4S6S-(β1 → and →4-L-Fuc-(3SO3-)-α1→, respectively.					
32835799	6	0	theme	repeating	862:870	arg1	sequences					872:880	the repeating sequences	858:880	the repeating sequences of FCS and SFII	858:896	As a result, the repeating sequences of FCS and SFII were →4)-[L-Fuc3S4S-(α1 → 3)]-D-GlcA-(β1 → 3)-D-GalNAc4S6S-(β1 → and →4-L-Fuc-(3SO3-)-α1→, respectively.					
32835799	11	1	from	cucumber	1420:1427	arg1	types					1377:1381	the structure types	1363:1381	the structure types of sulfated polysaccharides from sea cucumber	1363:1427	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	11	1	from	cucumber	1420:1427	arg1	polysaccharides					1395:1409	sulfated polysaccharides	1386:1409	sulfated polysaccharides from sea cucumber	1386:1427	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	11	2	theme	sulfated	1386:1393	arg1	polysaccharides					1395:1409	sulfated polysaccharides	1386:1409	sulfated polysaccharides from sea cucumber	1386:1427	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	0	3	theme	polysaccharides	88:102	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	0	3	theme	polysaccharides	88:102	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	0	3	theme	polysaccharides	88:102	arg1	activities					60:69	anticoagulant activities	46:69	anticoagulant activities	46:69	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	7	4	theme	SFI	1028:1030	arg1	structure					1015:1023	The primary structure	1003:1023	The primary structure of SFI	1003:1030	The primary structure of SFI was →3)-L-Fuc2S4S-(α1 → 4)-L-Fuc-(α1 → 3)-L-Fuc2S-(α1 → 4)-L-Fuc-(α1→.					
32835799	7	4	theme	SFI	1028:1030	arg1	α1→					1098:1100	α1→	1098:1100	α1→	1098:1100	The primary structure of SFI was →3)-L-Fuc2S4S-(α1 → 4)-L-Fuc-(α1 → 3)-L-Fuc2S-(α1 → 4)-L-Fuc-(α1→.					
32835799	7	4	theme	SFI	1028:1030	arg1	-L-Fuc-					1090:1096	-L-Fuc-	1090:1096	-L-Fuc-	1090:1096	The primary structure of SFI was →3)-L-Fuc2S4S-(α1 → 4)-L-Fuc-(α1 → 3)-L-Fuc2S-(α1 → 4)-L-Fuc-(α1→.					
32835799	10	5	theme	potent	1248:1253	arg1	activity					1269:1276	potent anticoagulant activity	1248:1276	potent anticoagulant activity	1248:1276	FCS and AG showed potent anticoagulant activity and intrinsic factor Xase inhibition activity.					
32835799	1	6	theme	unique	199:204	arg1	structure					215:223	unique chemical structure	199:223	unique chemical structure	199:223	Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities.					
32835799	11	7	theme	structure	1367:1375	arg1	types					1377:1381	the structure types	1363:1381	the structure types of sulfated polysaccharides from sea cucumber	1363:1427	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	4	8	theme	SFI	604:606	arg1	470.6					631:635	470.6	631:635	470.6	631:635	The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively.					
32835799	4	8	theme	SFI	604:606	arg1	Mw					598:599	The Mw	594:599	The Mw of SFI, SFII, FCS and AG	594:624	The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively.					
32835799	0	9	from	Purification	0:11	arg1	cucumber					113:120	sea cucumber	109:120	sea cucumber	109:120	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	7	10	theme	primary	1007:1013	arg1	structure					1015:1023	The primary structure	1003:1023	The primary structure of SFI	1003:1030	The primary structure of SFI was →3)-L-Fuc2S4S-(α1 → 4)-L-Fuc-(α1 → 3)-L-Fuc2S-(α1 → 4)-L-Fuc-(α1→.					
32835799	7	10	theme	primary	1007:1013	arg1	α1→					1098:1100	α1→	1098:1100	α1→	1098:1100	The primary structure of SFI was →3)-L-Fuc2S4S-(α1 → 4)-L-Fuc-(α1 → 3)-L-Fuc2S-(α1 → 4)-L-Fuc-(α1→.					
32835799	7	10	theme	primary	1007:1013	arg1	-L-Fuc-					1090:1096	-L-Fuc-	1090:1096	-L-Fuc-	1090:1096	The primary structure of SFI was →3)-L-Fuc2S4S-(α1 → 4)-L-Fuc-(α1 → 3)-L-Fuc2S-(α1 → 4)-L-Fuc-(α1→.					
32835799	0	11	dep	fuscopunctata	133:145	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	0	11	dep	fuscopunctata	133:145	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	0	11	dep	fuscopunctata	133:145	arg1	activities					60:69	anticoagulant activities	46:69	anticoagulant activities	46:69	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	0	12	theme	sea	109:111	arg1	cucumber					113:120	sea cucumber	109:120	sea cucumber	109:120	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	10	13	theme	factor	1292:1297	arg1	activity					1315:1322	intrinsic factor Xase inhibition activity	1282:1322	intrinsic factor Xase inhibition activity	1282:1322	FCS and AG showed potent anticoagulant activity and intrinsic factor Xase inhibition activity.					
32835799	11	14	theme	polysaccharides	1395:1409	arg1	types					1377:1381	the structure types	1363:1381	the structure types of sulfated polysaccharides from sea cucumber	1363:1427	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	2	15	theme	body	328:331	arg1	wall					333:336	the body wall	324:336	the body wall of Holothuria fuscopunctata	324:364	In this study, four sulfated polysaccharides were purified from the body wall of Holothuria fuscopunctata by anion exchange chromatography and chemical depolymerization.					
32835799	4	16	theme	SFII	609:612	arg1	470.6					631:635	470.6	631:635	470.6	631:635	The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively.					
32835799	4	16	theme	SFII	609:612	arg1	Mw					598:599	The Mw	594:599	The Mw of SFI, SFII, FCS and AG	594:624	The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively.					
32835799	1	17	contain	possess	191:197	arg1	polysaccharides					157:171	Sulfated polysaccharides	148:171	Sulfated polysaccharides from sea cucumber	148:189	Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities.					
32835799	1	17	contain	possess	191:197	arg2	activities					248:257	various biological activities	229:257	various biological activities	229:257	Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities.					
32835799	1	17	contain	possess	191:197	arg2	structure					215:223	unique chemical structure	199:223	unique chemical structure	199:223	Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities.					
32835799	3	18	dep	fucan	463:467	arg1	SFII					475:478	SFII	475:478	SFII	475:478	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	3	18	dep	fucan	463:467	arg1	SFI					470:472	SFI	470:472	SFI	470:472	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	1	19	from	cucumber	182:189	arg1	polysaccharides					157:171	Sulfated polysaccharides	148:171	Sulfated polysaccharides from sea cucumber	148:189	Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities.					
32835799	3	20	theme	physicochemical	553:567	arg1	analyses					584:591	physicochemical and structural analyses	553:591	physicochemical and structural analyses	553:591	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	11	21	from	knowledge	1350:1358	arg1	types					1377:1381	the structure types	1363:1381	the structure types of sulfated polysaccharides from sea cucumber	1363:1427	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	1	22	theme	chemical	206:213	arg1	structure					215:223	unique chemical structure	199:223	unique chemical structure	199:223	Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities.					
32835799	10	23	theme	intrinsic	1282:1290	arg1	activity					1315:1322	intrinsic factor Xase inhibition activity	1282:1322	intrinsic factor Xase inhibition activity	1282:1322	FCS and AG showed potent anticoagulant activity and intrinsic factor Xase inhibition activity.					
32835799	3	24	theme	fucosylated	482:492	arg1	sulfate					506:512	fucosylated chondroitin sulfate	482:512	fucosylated chondroitin sulfate (FCS)	482:518	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	3	24	theme	fucosylated	482:492	arg1	They					430:433	They	430:433	They	430:433	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	3	24	theme	fucosylated	482:492	arg1	FCS					515:517	FCS	515:517	FCS	515:517	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	9	25	theme	anticoagulant	1172:1184	arg1	activities					1186:1195	Their anticoagulant activities	1166:1195	Their anticoagulant activities	1166:1195	Their anticoagulant activities were further evaluated in vitro.					
32835799	0	26	from	activities	60:69	arg1	cucumber					113:120	sea cucumber	109:120	sea cucumber	109:120	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	5	27	theme	spectroscopy	822:833	arg1	analysis					835:842	1D/2D NMR spectroscopy analysis	812:842	1D/2D NMR spectroscopy analysis	812:842	Their primary structures were clarified both by monosaccharide composition and 1D/2D NMR spectroscopy analysis.					
32835799	3	28	theme	sulfated	454:461	arg1	fucan					463:467	sulfated fucan	454:467	sulfated fucan (SFI, SFII)	454:479	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	3	28	theme	sulfated	454:461	arg1	They					430:433	They	430:433	They	430:433	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	11	29	from	illustration	1441:1452	arg1	types					1377:1381	the structure types	1363:1381	the structure types of sulfated polysaccharides from sea cucumber	1363:1427	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	2	30	theme	fuscopunctata	352:364	arg1	wall					333:336	the body wall	324:336	the body wall of Holothuria fuscopunctata	324:364	In this study, four sulfated polysaccharides were purified from the body wall of Holothuria fuscopunctata by anion exchange chromatography and chemical depolymerization.					
32835799	8	31	theme	sulfated	1107:1114	arg1	AG					1116:1117	The sulfated AG	1103:1117	The sulfated AG	1103:1117	The sulfated AG was composed of four types of monosaccharides.					
32835799	1	32	theme	various	229:235	arg1	activities					248:257	various biological activities	229:257	various biological activities	229:257	Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities.					
32835799	11	33	from	types	1377:1381	arg1	cucumber					1420:1427	sea cucumber	1416:1427	sea cucumber	1416:1427	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	10	34	theme	Xase	1299:1302	arg1	activity					1315:1322	intrinsic factor Xase inhibition activity	1282:1322	intrinsic factor Xase inhibition activity	1282:1322	FCS and AG showed potent anticoagulant activity and intrinsic factor Xase inhibition activity.					
32835799	10	35	theme	anticoagulant	1255:1267	arg1	activity					1269:1276	potent anticoagulant activity	1248:1276	potent anticoagulant activity	1248:1276	FCS and AG showed potent anticoagulant activity and intrinsic factor Xase inhibition activity.					
32835799	2	36	theme	Holothuria	341:350	arg1	fuscopunctata					352:364	Holothuria fuscopunctata	341:364	Holothuria fuscopunctata	341:364	In this study, four sulfated polysaccharides were purified from the body wall of Holothuria fuscopunctata by anion exchange chromatography and chemical depolymerization.					
32835799	11	37	theme	sea	1416:1418	arg1	cucumber					1420:1427	sea cucumber	1416:1427	sea cucumber	1416:1427	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	1	38	theme	biological	237:246	arg1	activities					248:257	various biological activities	229:257	various biological activities	229:257	Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities.					
32835799	0	39	theme	structural	14:23	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	2	40	theme	chemical	403:410	arg1	depolymerization					412:427	chemical depolymerization	403:427	chemical depolymerization	403:427	In this study, four sulfated polysaccharides were purified from the body wall of Holothuria fuscopunctata by anion exchange chromatography and chemical depolymerization.					
32835799	4	41	theme	sulfate	667:673	arg1	content					675:681	the sulfate content	663:681	the sulfate content	663:681	The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively.					
32835799	4	41	theme	sulfate	667:673	arg1	%					692:692	21.40%	687:692	21.40%	687:692	The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively.					
32835799	0	42	theme	anticoagulant	46:58	arg1	activities					60:69	anticoagulant activities	46:69	anticoagulant activities	46:69	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	3	43	theme	chondroitin	494:504	arg1	sulfate					506:512	fucosylated chondroitin sulfate	482:512	fucosylated chondroitin sulfate (FCS)	482:518	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	3	43	theme	chondroitin	494:504	arg1	They					430:433	They	430:433	They	430:433	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	3	43	theme	chondroitin	494:504	arg1	FCS					515:517	FCS	515:517	FCS	515:517	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	5	44	theme	1D/2D	812:816	arg1	analysis					835:842	1D/2D NMR spectroscopy analysis	812:842	1D/2D NMR spectroscopy analysis	812:842	Their primary structures were clarified both by monosaccharide composition and 1D/2D NMR spectroscopy analysis.					
32835799	6	45	theme	SFII	893:896	arg1	-α1→					983:986	-α1→	983:986	-α1→	983:986	As a result, the repeating sequences of FCS and SFII were →4)-[L-Fuc3S4S-(α1 → 3)]-D-GlcA-(β1 → 3)-D-GalNAc4S6S-(β1 → and →4-L-Fuc-(3SO3-)-α1→, respectively.					
32835799	6	45	theme	SFII	893:896	arg1	sequences					872:880	the repeating sequences	858:880	the repeating sequences of FCS and SFII	858:896	As a result, the repeating sequences of FCS and SFII were →4)-[L-Fuc3S4S-(α1 → 3)]-D-GlcA-(β1 → 3)-D-GalNAc4S6S-(β1 → and →4-L-Fuc-(3SO3-)-α1→, respectively.					
32835799	7	46	dep	-L-Fuc-	1090:1096	arg1	structure					1015:1023	The primary structure	1003:1023	The primary structure of SFI	1003:1030	The primary structure of SFI was →3)-L-Fuc2S4S-(α1 → 4)-L-Fuc-(α1 → 3)-L-Fuc2S-(α1 → 4)-L-Fuc-(α1→.					
32835799	7	46	dep	-L-Fuc-	1090:1096	arg1	α1→					1098:1100	α1→	1098:1100	α1→	1098:1100	The primary structure of SFI was →3)-L-Fuc2S4S-(α1 → 4)-L-Fuc-(α1 → 3)-L-Fuc2S-(α1 → 4)-L-Fuc-(α1→.					
32835799	7	46	dep	-L-Fuc-	1090:1096	arg1	-L-Fuc-					1090:1096	-L-Fuc-	1090:1096	-L-Fuc-	1090:1096	The primary structure of SFI was →3)-L-Fuc2S4S-(α1 → 4)-L-Fuc-(α1 → 3)-L-Fuc2S-(α1 → 4)-L-Fuc-(α1→.					
32835799	10	47	theme	inhibition	1304:1313	arg1	activity					1315:1322	intrinsic factor Xase inhibition activity	1282:1322	intrinsic factor Xase inhibition activity	1282:1322	FCS and AG showed potent anticoagulant activity and intrinsic factor Xase inhibition activity.					
32835799	5	48	theme	monosaccharide	781:794	arg1	composition					796:806	monosaccharide composition	781:806	monosaccharide composition	781:806	Their primary structures were clarified both by monosaccharide composition and 1D/2D NMR spectroscopy analysis.					
32835799	3	49	theme	sulfated	524:531	arg1	AG					546:547	AG	546:547	AG	546:547	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	3	49	theme	sulfated	524:531	arg1	aminoglycan					533:543	sulfated aminoglycan	524:543	sulfated aminoglycan (AG)	524:548	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	3	49	theme	sulfated	524:531	arg1	They					430:433	They	430:433	They	430:433	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	5	50	theme	primary	739:745	arg1	structures					747:756	Their primary structures	733:756	Their primary structures	733:756	Their primary structures were clarified both by monosaccharide composition and 1D/2D NMR spectroscopy analysis.					
32835799	6	51	theme	FCS	885:887	arg1	-α1→					983:986	-α1→	983:986	-α1→	983:986	As a result, the repeating sequences of FCS and SFII were →4)-[L-Fuc3S4S-(α1 → 3)]-D-GlcA-(β1 → 3)-D-GalNAc4S6S-(β1 → and →4-L-Fuc-(3SO3-)-α1→, respectively.					
32835799	6	51	theme	FCS	885:887	arg1	sequences					872:880	the repeating sequences	858:880	the repeating sequences of FCS and SFII	858:896	As a result, the repeating sequences of FCS and SFII were →4)-[L-Fuc3S4S-(α1 → 3)]-D-GlcA-(β1 → 3)-D-GalNAc4S6S-(β1 → and →4-L-Fuc-(3SO3-)-α1→, respectively.					
32835799	1	52	theme	Sulfated	148:155	arg1	polysaccharides					157:171	Sulfated polysaccharides	148:171	Sulfated polysaccharides from sea cucumber	148:189	Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities.					
32835799	11	53	theme	further	1433:1439	arg1	illustration					1441:1452	further illustration	1433:1452	further illustration of their functionality	1433:1475	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	5	54	dep	composition	796:806	arg1	both					773:776	both	773:776	both	773:776	Their primary structures were clarified both by monosaccharide composition and 1D/2D NMR spectroscopy analysis.					
32835799	3	55	gly	fucosylated	482:492	arg1	sulfate					506:512	fucosylated chondroitin sulfate	482:512	fucosylated chondroitin sulfate (FCS)	482:518	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	3	55	gly	fucosylated	482:492	arg1	They					430:433	They	430:433	They	430:433	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	3	55	gly	fucosylated	482:492	arg1	FCS					515:517	FCS	515:517	FCS	515:517	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	11	56	theme	functionality	1463:1475	arg1	illustration					1441:1452	further illustration	1433:1452	further illustration of their functionality	1433:1475	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	11	56	theme	functionality	1463:1475	arg1	knowledge					1350:1358	the knowledge	1346:1358	the knowledge on the structure types of sulfated polysaccharides from sea cucumber	1346:1427	These results expand the knowledge on the structure types of sulfated polysaccharides from sea cucumber and further illustration of their functionality.					
32835799	2	57	theme	exchange	375:382	arg1	chromatography					384:397	anion exchange chromatography	369:397	anion exchange chromatography	369:397	In this study, four sulfated polysaccharides were purified from the body wall of Holothuria fuscopunctata by anion exchange chromatography and chemical depolymerization.					
32835799	0	58	from	cucumber	113:120	arg1	polysaccharides					88:102	four sulfated polysaccharides	74:102	four sulfated polysaccharides from sea cucumber	74:120	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	0	58	from	cucumber	113:120	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	0	58	from	cucumber	113:120	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	0	58	from	cucumber	113:120	arg1	activities					60:69	anticoagulant activities	46:69	anticoagulant activities	46:69	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	3	59	theme	structural	573:582	arg1	analyses					584:591	physicochemical and structural analyses	553:591	physicochemical and structural analyses	553:591	They were identified as sulfated fucan (SFI, SFII), fucosylated chondroitin sulfate (FCS) and sulfated aminoglycan (AG) by physicochemical and structural analyses.					
32835799	2	60	theme	anion	369:373	arg1	chromatography					384:397	anion exchange chromatography	369:397	anion exchange chromatography	369:397	In this study, four sulfated polysaccharides were purified from the body wall of Holothuria fuscopunctata by anion exchange chromatography and chemical depolymerization.					
32835799	4	61	theme	FCS	615:617	arg1	470.6					631:635	470.6	631:635	470.6	631:635	The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively.					
32835799	4	61	theme	FCS	615:617	arg1	Mw					598:599	The Mw	594:599	The Mw of SFI, SFII, FCS and AG	594:624	The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively.					
32835799	0	62	theme	sulfated	79:86	arg1	polysaccharides					88:102	four sulfated polysaccharides	74:102	four sulfated polysaccharides from sea cucumber	74:120	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	1	63	theme	sea	178:180	arg1	cucumber					182:189	sea cucumber	178:189	sea cucumber	178:189	Sulfated polysaccharides from sea cucumber possess unique chemical structure and various biological activities.					
32835799	5	64	theme	NMR	818:820	arg1	analysis					835:842	1D/2D NMR spectroscopy analysis	812:842	1D/2D NMR spectroscopy analysis	812:842	Their primary structures were clarified both by monosaccharide composition and 1D/2D NMR spectroscopy analysis.					
32835799	0	65	from	characterization	25:40	arg1	cucumber					113:120	sea cucumber	109:120	sea cucumber	109:120	Purification, structural characterization and anticoagulant activities of four sulfated polysaccharides from sea cucumber Holothuria fuscopunctata.					
32835799	2	66	theme	sulfated	280:287	arg1	polysaccharides					289:303	four sulfated polysaccharides	275:303	four sulfated polysaccharides	275:303	In this study, four sulfated polysaccharides were purified from the body wall of Holothuria fuscopunctata by anion exchange chromatography and chemical depolymerization.					
32835799	8	67	theme	monosaccharides	1149:1163	arg1	types					1140:1144	four types	1135:1144	four types of monosaccharides	1135:1163	The sulfated AG was composed of four types of monosaccharides.					
32835799	4	68	theme	AG	623:624	arg1	470.6					631:635	470.6	631:635	470.6	631:635	The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively.					
32835799	4	68	theme	AG	623:624	arg1	Mw					598:599	The Mw	594:599	The Mw of SFI, SFII, FCS and AG	594:624	The Mw of SFI, SFII, FCS and AG were 470.6, 36.8, 42.6, 39.6 kDa and the sulfate content was 21.40%, 35.86%, 33.70%, 35.70%, respectively.					
34246676	6	0	dep	-l-FucS-α	853:861	arg1	%					894:894	10%	892:894	10%	892:894	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	0	dep	-l-FucS-α	853:861	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	0	dep	-l-FucS-α	853:861	arg1	Fuc4S					924:928	Fuc4S	924:928	Fuc4S	924:928	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	0	dep	-l-FucS-α	853:861	arg1	Fuc2S					898:902	Fuc2S	898:902	Fuc2S	898:902	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	0	dep	-l-FucS-α	853:861	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	0	dep	-l-FucS-α	853:861	arg1	Fuc0S					911:915	Fuc0S	911:915	Fuc0S	911:915	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	0	dep	-l-FucS-α	853:861	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	0	dep	-l-FucS-α	853:861	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	7	1	dep	report	1196:1201	arg1	obtained					1248:1255	obtained	1248:1255	were obtained from the same sea cucumber species	1243:1290	This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species.					
34246676	5	2	theme	FSII	645:648	arg1	23.2 kDa					719:726	23.2 kDa	719:726	23.2 kDa	719:726	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	2	theme	FSII	645:648	arg1	6.12 kDa					732:739	6.12 kDa	732:739	6.12 kDa	732:739	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	2	theme	FSII	645:648	arg1	weight					625:630	The molecular weight	611:630	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV	611:669	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	2	theme	FSII	645:648	arg1	81.0 kDa					699:706	81.0 kDa	699:706	81.0 kDa	699:706	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	2	theme	FSII	645:648	arg1	Mw					633:634	Mw	633:634	Mw	633:634	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	2	theme	FSII	645:648	arg1	82.0 kDa					709:716	82.0 kDa	709:716	82.0 kDa	709:716	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	7	3	theme	same	1266:1269	arg1	species					1284:1290	the same sea cucumber species	1262:1290	the same sea cucumber species	1262:1290	This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species.					
34246676	6	4	theme	α1→4	1122:1125	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	4	theme	α1→4	1122:1125	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	4	theme	α1→4	1122:1125	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	4	theme	α1→4	1122:1125	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	2	5	from	mollis	460:465	arg1	purification					363:374	the purification	359:374	the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis	359:465	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	10	6	theme	sulfation	1573:1581	arg1	factors					1612:1618	the crucial factors	1600:1618	the crucial factors for the activity	1600:1635	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	10	6	theme	sulfation	1573:1581	arg1	degree					1583:1588	sulfation degree	1573:1588	sulfation degree	1573:1588	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	10	6	theme	sulfation	1573:1581	arg1	weight					1562:1567	molecular weight	1552:1567	molecular weight	1552:1567	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	5	7	theme	molecular	615:623	arg1	23.2 kDa					719:726	23.2 kDa	719:726	23.2 kDa	719:726	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	7	theme	molecular	615:623	arg1	6.12 kDa					732:739	6.12 kDa	732:739	6.12 kDa	732:739	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	7	theme	molecular	615:623	arg1	weight					625:630	The molecular weight	611:630	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV	611:669	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	7	theme	molecular	615:623	arg1	81.0 kDa					699:706	81.0 kDa	699:706	81.0 kDa	699:706	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	7	theme	molecular	615:623	arg1	Mw					633:634	Mw	633:634	Mw	633:634	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	7	theme	molecular	615:623	arg1	82.0 kDa					709:716	82.0 kDa	709:716	82.0 kDa	709:716	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	1	8	theme	characteristic	175:188	arg1	structures					190:199	characteristic structures	175:199	characteristic structures	175:199	Fucan sulfates from echinoderm possess characteristic structures and various biological activities.					
34246676	4	9	theme	conductimetric	588:601	arg1	method					603:608	a conductimetric method	586:608	a conductimetric method	586:608	Their sulfate content was determined by a conductimetric method.					
34246676	9	10	theme	similar	1450:1456	arg1	potency					1458:1464	the similar potency	1446:1464	the similar potency	1446:1464	Among them, FSII, FSIII and FSIV showed the similar potency, while FSI owned the strongest.					
34246676	7	11	theme	first	1190:1194	arg1	This					1178:1181	This	1178:1181	This	1178:1181	This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species.					
34246676	7	11	theme	first	1190:1194	arg1	report					1196:1201	the first report	1186:1201	the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species	1186:1290	This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species.					
34246676	5	12	theme	FSIII	651:655	arg1	23.2 kDa					719:726	23.2 kDa	719:726	23.2 kDa	719:726	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	12	theme	FSIII	651:655	arg1	6.12 kDa					732:739	6.12 kDa	732:739	6.12 kDa	732:739	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	12	theme	FSIII	651:655	arg1	weight					625:630	The molecular weight	611:630	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV	611:669	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	12	theme	FSIII	651:655	arg1	81.0 kDa					699:706	81.0 kDa	699:706	81.0 kDa	699:706	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	12	theme	FSIII	651:655	arg1	Mw					633:634	Mw	633:634	Mw	633:634	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	12	theme	FSIII	651:655	arg1	82.0 kDa					709:716	82.0 kDa	709:716	82.0 kDa	709:716	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	6	13	theme	α1 → 4	1154:1159	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	13	theme	α1 → 4	1154:1159	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	13	theme	α1 → 4	1154:1159	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	13	theme	α1 → 4	1154:1159	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	10	14	theme	molecular	1552:1560	arg1	factors					1612:1618	the crucial factors	1600:1618	the crucial factors for the activity	1600:1635	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	10	14	theme	molecular	1552:1560	arg1	weight					1562:1567	molecular weight	1552:1567	molecular weight	1552:1567	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	10	14	theme	molecular	1552:1560	arg1	degree					1583:1588	sulfation degree	1573:1588	sulfation degree	1573:1588	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	0	15	theme	structural	78:87	arg1	characterization					89:104	structural characterization	78:104	structural characterization	78:104	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	0	15	theme	structural	78:87	arg1	sulfates					20:27	Five distinct fucan sulfates	0:27	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.	0:134	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	7	16	theme	sulfates	1234:1241	arg1	diversity					1215:1223	such a diversity	1208:1223	such a diversity of fucan sulfates	1208:1241	This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species.					
34246676	0	17	theme	anticoagulant	110:122	arg1	activities					124:133	anticoagulant activities	110:133	anticoagulant activities	110:133	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	1	18	contain	possess	167:173	arg2	structures					190:199	characteristic structures	175:199	characteristic structures	175:199	Fucan sulfates from echinoderm possess characteristic structures and various biological activities.					
34246676	1	18	contain	possess	167:173	arg1	sulfates					142:149	Fucan sulfates	136:149	Fucan sulfates from echinoderm	136:165	Fucan sulfates from echinoderm possess characteristic structures and various biological activities.					
34246676	1	18	contain	possess	167:173	arg2	activities					224:233	various biological activities	205:233	various biological activities	205:233	Fucan sulfates from echinoderm possess characteristic structures and various biological activities.					
34246676	1	19	from	echinoderm	156:165	arg1	sulfates					142:149	Fucan sulfates	136:149	Fucan sulfates from echinoderm	136:165	Fucan sulfates from echinoderm possess characteristic structures and various biological activities.					
34246676	2	20	theme	exclusion	326:334	arg1	chromatography					336:349	size exclusion chromatography	321:349	size exclusion chromatography	321:349	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	6	21	theme	α1 → 4	1105:1110	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	21	theme	α1 → 4	1105:1110	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	21	theme	α1 → 4	1105:1110	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	21	theme	α1 → 4	1105:1110	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	22	theme	NMR	765:767	arg1	analysis					783:790	Detailed NMR spectroscopic analysis	756:790	Detailed NMR spectroscopic analysis	756:790	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	2	23	theme	size	321:324	arg1	chromatography					336:349	size exclusion chromatography	321:349	size exclusion chromatography	321:349	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	0	24	theme	distinct	5:12	arg1	Purification					64:75	Purification	64:75	Purification	64:75	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	0	24	theme	distinct	5:12	arg1	characterization					89:104	structural characterization	78:104	structural characterization	78:104	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	0	24	theme	distinct	5:12	arg1	sulfates					20:27	Five distinct fucan sulfates	0:27	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.	0:134	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	5	25	theme	FSI	640:642	arg1	23.2 kDa					719:726	23.2 kDa	719:726	23.2 kDa	719:726	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	25	theme	FSI	640:642	arg1	6.12 kDa					732:739	6.12 kDa	732:739	6.12 kDa	732:739	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	25	theme	FSI	640:642	arg1	weight					625:630	The molecular weight	611:630	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV	611:669	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	25	theme	FSI	640:642	arg1	81.0 kDa					699:706	81.0 kDa	699:706	81.0 kDa	699:706	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	25	theme	FSI	640:642	arg1	Mw					633:634	Mw	633:634	Mw	633:634	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	25	theme	FSI	640:642	arg1	82.0 kDa					709:716	82.0 kDa	709:716	82.0 kDa	709:716	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	6	26	theme	Detailed	756:763	arg1	analysis					783:790	Detailed NMR spectroscopic analysis	756:790	Detailed NMR spectroscopic analysis	756:790	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	27	theme	-l-Fuc2S-	975:983	arg1	-l-Fuc0S/3S-					992:1003	→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→	955:1007	→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→	955:1007	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	1	28	theme	various	205:211	arg1	activities					224:233	various biological activities	205:233	various biological activities	205:233	Fucan sulfates from echinoderm possess characteristic structures and various biological activities.					
34246676	2	29	theme	Pattalus	451:458	arg1	mollis					460:465	the sea cucumber Pattalus mollis	434:465	the sea cucumber Pattalus mollis	434:465	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	6	30	theme	FSI	833:835	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	30	theme	FSI	833:835	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	30	theme	FSI	833:835	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	30	theme	FSI	833:835	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	1	31	theme	biological	213:222	arg1	activities					224:233	various biological activities	205:233	various biological activities	205:233	Fucan sulfates from echinoderm possess characteristic structures and various biological activities.					
34246676	2	32	theme	cucumber	442:449	arg1	mollis					460:465	the sea cucumber Pattalus mollis	434:465	the sea cucumber Pattalus mollis	434:465	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	10	33	theme	Structure-activity	1498:1515	arg1	relationships					1517:1529	Structure-activity relationships	1498:1529	Structure-activity relationships analysis	1498:1538	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	6	34	theme	spectroscopic	769:781	arg1	analysis					783:790	Detailed NMR spectroscopic analysis	756:790	Detailed NMR spectroscopic analysis	756:790	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	35	theme	α1 → 4	968:973	arg1	-l-Fuc0S/3S-					992:1003	→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→	955:1007	→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→	955:1007	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	3	36	theme	composition	477:487	arg1	analysis					489:496	Chemical composition analysis	468:496	Chemical composition analysis	468:496	Chemical composition analysis showed that they were all composed of l-fucose.					
34246676	2	37	theme	sea	438:440	arg1	mollis					460:465	the sea cucumber Pattalus mollis	434:465	the sea cucumber Pattalus mollis	434:465	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	7	38	theme	sea	1271:1273	arg1	species					1284:1290	the same sea cucumber species	1262:1290	the same sea cucumber species	1262:1290	This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species.					
34246676	6	39	theme	-l-Fuc2S3S-	1093:1103	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	39	theme	-l-Fuc2S3S-	1093:1103	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	39	theme	-l-Fuc2S3S-	1093:1103	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	39	theme	-l-Fuc2S3S-	1093:1103	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	40	dep	Fuc2S4S	883:889	arg1	1→					863:864	1→	863:864	1→	863:864	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	2	41	dep	sulfates	390:397	arg1	FSIV					418:421	FSIV	418:421	FSIV	418:421	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	2	41	dep	sulfates	390:397	arg1	FSIII					411:415	FSIII	411:415	FSIII	411:415	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	2	41	dep	sulfates	390:397	arg1	FSII					405:408	FSII	405:408	FSII	405:408	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	2	41	dep	sulfates	390:397	arg1	FSV					424:426	FSV	424:426	FSV	424:426	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	2	41	dep	sulfates	390:397	arg1	FSI					400:402	FSI	400:402	FSI	400:402	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	6	42	theme	FSIII	945:949	arg1	-l-Fuc0S/3S-					992:1003	→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→	955:1007	→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→	955:1007	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	0	43	theme	sea	34:36	arg1	mollis					56:61	sea cucumber Pattalus mollis	34:61	sea cucumber Pattalus mollis	34:61	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	6	44	theme	FSII	841:844	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	44	theme	FSII	841:844	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	44	theme	FSII	841:844	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	44	theme	FSII	841:844	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	45	theme	-l-Fuc2S-	1144:1152	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	45	theme	-l-Fuc2S-	1144:1152	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	45	theme	-l-Fuc2S-	1144:1152	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	45	theme	-l-Fuc2S-	1144:1152	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	46	theme	α1 → 4	1086:1091	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	46	theme	α1 → 4	1086:1091	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	46	theme	α1 → 4	1086:1091	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	46	theme	α1 → 4	1086:1091	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	7	47	theme	fucan	1228:1232	arg1	sulfates					1234:1241	fucan sulfates	1228:1241	fucan sulfates	1228:1241	This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species.					
34246676	3	48	theme	Chemical	468:475	arg1	analysis					489:496	Chemical composition analysis	468:496	Chemical composition analysis	468:496	Chemical composition analysis showed that they were all composed of l-fucose.					
34246676	0	49	theme	Pattalus	47:54	arg1	mollis					56:61	sea cucumber Pattalus mollis	34:61	sea cucumber Pattalus mollis	34:61	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	5	50	theme	FSIV	658:661	arg1	23.2 kDa					719:726	23.2 kDa	719:726	23.2 kDa	719:726	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	50	theme	FSIV	658:661	arg1	6.12 kDa					732:739	6.12 kDa	732:739	6.12 kDa	732:739	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	50	theme	FSIV	658:661	arg1	weight					625:630	The molecular weight	611:630	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV	611:669	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	50	theme	FSIV	658:661	arg1	81.0 kDa					699:706	81.0 kDa	699:706	81.0 kDa	699:706	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	50	theme	FSIV	658:661	arg1	Mw					633:634	Mw	633:634	Mw	633:634	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	50	theme	FSIV	658:661	arg1	82.0 kDa					709:716	82.0 kDa	709:716	82.0 kDa	709:716	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	0	51	from	mollis	56:61	arg1	Purification					64:75	Purification	64:75	Purification	64:75	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	0	51	from	mollis	56:61	arg1	characterization					89:104	structural characterization	78:104	structural characterization	78:104	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	0	51	from	mollis	56:61	arg1	sulfates					20:27	Five distinct fucan sulfates	0:27	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.	0:134	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	6	52	theme	-l-Fuc2S-	958:966	arg1	-l-Fuc0S/3S-					992:1003	→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→	955:1007	→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→	955:1007	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	0	53	theme	cucumber	38:45	arg1	mollis					56:61	sea cucumber Pattalus mollis	34:61	sea cucumber Pattalus mollis	34:61	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	8	54	theme	Biological	1293:1302	arg1	activity					1304:1311	Biological activity	1293:1311	Biological activity	1293:1311	Biological activity showed that FSI, FSII, FSIII and FSIV exhibited potent anticoagulant by prolonging the APTT.					
34246676	6	55	theme	-l-Fuc2S3S-	1074:1084	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	55	theme	-l-Fuc2S3S-	1074:1084	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	55	theme	-l-Fuc2S3S-	1074:1084	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	55	theme	-l-Fuc2S3S-	1074:1084	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	2	56	theme	comprehensive	244:256	arg1	chromatography					336:349	size exclusion chromatography	321:349	size exclusion chromatography	321:349	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	2	56	theme	comprehensive	244:256	arg1	enzymolysis					276:286	enzymolysis	276:286	enzymolysis	276:286	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	2	56	theme	comprehensive	244:256	arg1	chromatography					302:315	ion-exchange chromatography	289:315	ion-exchange chromatography	289:315	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	2	56	theme	comprehensive	244:256	arg1	methods					258:264	comprehensive methods	244:264	comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography	244:349	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	2	57	theme	sulfates	390:397	arg1	purification					363:374	the purification	359:374	the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis	359:465	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	4	58	theme	sulfate	552:558	arg1	content					560:566	Their sulfate content	546:566	Their sulfate content	546:566	Their sulfate content was determined by a conductimetric method.					
34246676	5	59	theme	FSV	667:669	arg1	23.2 kDa					719:726	23.2 kDa	719:726	23.2 kDa	719:726	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	59	theme	FSV	667:669	arg1	6.12 kDa					732:739	6.12 kDa	732:739	6.12 kDa	732:739	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	59	theme	FSV	667:669	arg1	weight					625:630	The molecular weight	611:630	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV	611:669	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	59	theme	FSV	667:669	arg1	81.0 kDa					699:706	81.0 kDa	699:706	81.0 kDa	699:706	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	59	theme	FSV	667:669	arg1	Mw					633:634	Mw	633:634	Mw	633:634	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	5	59	theme	FSV	667:669	arg1	82.0 kDa					709:716	82.0 kDa	709:716	82.0 kDa	709:716	The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively.					
34246676	0	60	dep	sulfates	20:27	arg1	Purification					64:75	Purification	64:75	Purification	64:75	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	0	60	dep	sulfates	20:27	arg1	characterization					89:104	structural characterization	78:104	structural characterization	78:104	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	0	60	dep	sulfates	20:27	arg1	activities					124:133	anticoagulant activities	110:133	anticoagulant activities	110:133	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	0	60	dep	sulfates	20:27	arg1	sulfates					20:27	Five distinct fucan sulfates	0:27	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.	0:134	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	1	61	theme	Fucan	136:140	arg1	sulfates					142:149	Fucan sulfates	136:149	Fucan sulfates from echinoderm	136:165	Fucan sulfates from echinoderm possess characteristic structures and various biological activities.					
34246676	10	62	theme	crucial	1604:1610	arg1	factors					1612:1618	the crucial factors	1600:1618	the crucial factors for the activity	1600:1635	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	10	62	theme	crucial	1604:1610	arg1	degree					1583:1588	sulfation degree	1573:1588	sulfation degree	1573:1588	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	10	62	theme	crucial	1604:1610	arg1	weight					1562:1567	molecular weight	1552:1567	molecular weight	1552:1567	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	6	63	dep	-l-Fuc2S-	1161:1169	arg1	α1 → 					1171:1175	α1 → 	1171:1175	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	64	theme	structural	810:819	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	64	theme	structural	810:819	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	64	theme	structural	810:819	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	64	theme	structural	810:819	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	10	65	theme	relationships	1517:1529	arg1	analysis					1531:1538	Structure-activity relationships analysis	1498:1538	Structure-activity relationships analysis	1498:1538	Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.					
34246676	8	66	theme	potent	1361:1366	arg1	anticoagulant					1368:1380	potent anticoagulant	1361:1380	potent anticoagulant	1361:1380	Biological activity showed that FSI, FSII, FSIII and FSIV exhibited potent anticoagulant by prolonging the APTT.					
34246676	2	67	theme	fucan	384:388	arg1	sulfates					390:397	five fucan sulfates	379:397	five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV)	379:427	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	2	68	theme	ion-exchange	289:300	arg1	chromatography					302:315	ion-exchange chromatography	289:315	ion-exchange chromatography	289:315	Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis.					
34246676	6	69	theme	α1 → 4	1137:1142	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	69	theme	α1 → 4	1137:1142	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	69	theme	α1 → 4	1137:1142	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	69	theme	α1 → 4	1137:1142	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	7	70	theme	cucumber	1275:1282	arg1	species					1284:1290	the same sea cucumber species	1262:1290	the same sea cucumber species	1262:1290	This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species.					
34246676	6	71	theme	-l-Fuc2S-	1127:1135	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	71	theme	-l-Fuc2S-	1127:1135	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	71	theme	-l-Fuc2S-	1127:1135	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	71	theme	-l-Fuc2S-	1127:1135	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	0	72	theme	fucan	14:18	arg1	Purification					64:75	Purification	64:75	Purification	64:75	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	0	72	theme	fucan	14:18	arg1	characterization					89:104	structural characterization	78:104	structural characterization	78:104	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	0	72	theme	fucan	14:18	arg1	sulfates					20:27	Five distinct fucan sulfates	0:27	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.	0:134	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.					
34246676	6	73	theme	-l-Fuc2S-	1112:1120	arg1	sequence					821:828	the structural sequence	806:828	the structural sequence of FSI and FSII	806:844	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	73	theme	-l-Fuc2S-	1112:1120	arg1	-l-FucS-α					853:861	-l-FucS-α	853:861	-l-FucS-α	853:861	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	73	theme	-l-Fuc2S-	1112:1120	arg1	-l-Fuc2S-					1161:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-	1071:1169	→4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 	1071:1175	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34246676	6	73	theme	-l-Fuc2S-	1112:1120	arg1	Fuc2S4S					883:889	Fuc2S4S	883:889	Fuc2S4S	883:889	Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was →3)-l-FucS-α(1→, where FucS were Fuc2S4S (10%), Fuc2S (44%), Fuc0S (10%), Fuc4S (36%), that of FSIII was →4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc0S/3S-(α1→, where Fuc0S and Fuc3S were in equal molar, and that FSIV was →4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S3S-(α1 → 4)-l-Fuc2S-(α1→4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → 4)-l-Fuc2S-(α1 → .					
34078286	0	0	theme	maize	72:76	arg1	exploitation					78:89	the maize exploitation	68:89	the maize exploitation	68:89	Elucidating the multifunctional role of the cell wall components in the maize exploitation.					
34078286	6	1	theme	wall	1331:1334	arg1	ideotype					1336:1343	no maize cell wall ideotype	1317:1343	no maize cell wall ideotype	1317:1343	CONCLUSIONS Results evidence that there is no maize cell wall ideotype among the tested for optimal performance for various uses, and maize plants should be specifically bred for each particular application.					
34078286	5	2	dep	pests	837:841	arg1	i					834:834	i	834:834	i	834:834	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	6	3	theme	cell	1326:1329	arg1	ideotype					1336:1343	no maize cell wall ideotype	1317:1343	no maize cell wall ideotype	1317:1343	CONCLUSIONS Results evidence that there is no maize cell wall ideotype among the tested for optimal performance for various uses, and maize plants should be specifically bred for each particular application.					
34078286	1	4	used	used	262:265	arg2	plant					249:253	the whole plant	239:253	the whole plant	239:253	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	6	5	theme	CONCLUSIONS	1274:1284	arg1	Results					1286:1292	CONCLUSIONS Results	1274:1292	CONCLUSIONS Results	1274:1292	CONCLUSIONS Results evidence that there is no maize cell wall ideotype among the tested for optimal performance for various uses, and maize plants should be specifically bred for each particular application.					
34078286	5	6	theme	low	913:915	arg1	content					931:937	low hemicellulose content	913:937	low hemicellulose content	913:937	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	2	7	dep	reduced	308:314	arg1	one					356:358	one	356:358	one	356:358	However, yield is reduced by pest damages, stem corn borers being one of the most important yield constraints.					
34078286	2	7	dep	reduced	308:314	arg1	constraints					388:398	the most important yield constraints	363:398	the most important yield constraints	363:398	However, yield is reduced by pest damages, stem corn borers being one of the most important yield constraints.					
34078286	5	8	theme	resistant	844:852	arg1	materials					854:862	resistant materials	844:862	resistant materials	844:862	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	9	theme	cell	966:969	arg1	walls					971:975	cell walls	966:975	cell walls with high cellulose content and high diferulate cross-linking	966:1037	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	6	10	dep	various	1390:1396	arg1	uses					1398:1401	uses	1398:1401	uses	1398:1401	CONCLUSIONS Results evidence that there is no maize cell wall ideotype among the tested for optimal performance for various uses, and maize plants should be specifically bred for each particular application.					
34078286	3	11	theme	biomass	475:481	arg1	quality					458:464	the quality	454:464	the quality of maize biomass	454:481	Overall, cell wall composition is key in determining the quality of maize biomass, as well as pest resistance.					
34078286	3	11	theme	biomass	475:481	arg1	resistance					500:509	pest resistance	495:509	pest resistance	495:509	Overall, cell wall composition is key in determining the quality of maize biomass, as well as pest resistance.					
34078286	6	12	theme	maize	1408:1412	arg1	plants					1414:1419	maize plants	1408:1419	maize plants	1408:1419	CONCLUSIONS Results evidence that there is no maize cell wall ideotype among the tested for optimal performance for various uses, and maize plants should be specifically bred for each particular application.					
34078286	3	13	theme	wall	415:418	arg1	composition					420:430	cell wall composition	410:430	cell wall composition	410:430	Overall, cell wall composition is key in determining the quality of maize biomass, as well as pest resistance.					
34078286	4	14	theme	ethanol	747:753	arg1	capacity					755:762	ethanol capacity	747:762	ethanol capacity	747:762	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	14	theme	ethanol	747:753	arg1	pests					740:744	pests	740:744	pests	740:744	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	5	15	from	subunits	1264:1271	arg1	richer					1252:1257	richer	1252:1257	richer	1252:1257	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	16	dep	highlighted	820:830	arg1	iii					1095:1097	iii	1095:1097	iii	1095:1097	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	16	dep	highlighted	820:830	arg1	ii					941:942	ii	941:942	ii	941:942	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	16	dep	highlighted	820:830	arg1	showing					958:964	showing	958:964	showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production	958:1091	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	4	17	theme	fractions	578:586	arg1	composition					544:554	the composition	540:554	the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes	540:670	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	2	18	theme	corn	338:341	arg1	borers					343:348	stem corn borers	333:348	stem corn borers	333:348	However, yield is reduced by pest damages, stem corn borers being one of the most important yield constraints.					
34078286	0	19	theme	cell	44:47	arg1	components					54:63	the cell wall components	40:63	the cell wall components	40:63	Elucidating the multifunctional role of the cell wall components in the maize exploitation.					
34078286	5	20	from	diferulates	1196:1206	arg1	poor					1160:1163	poor	1160:1163	poor	1160:1163	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	21	theme	cell	873:876	arg1	walls					878:882	cell walls	873:882	cell walls	873:882	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	2	22	theme	stem	333:336	arg1	borers					343:348	stem corn borers	333:348	stem corn borers	333:348	However, yield is reduced by pest damages, stem corn borers being one of the most important yield constraints.					
34078286	2	23	theme	yield	382:386	arg1	constraints					388:398	the most important yield constraints	363:398	the most important yield constraints	363:398	However, yield is reduced by pest damages, stem corn borers being one of the most important yield constraints.					
34078286	5	24	theme	high	982:985	arg1	content					997:1003	high cellulose content	982:1003	high cellulose content	982:1003	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	25	theme	higher	1051:1056	arg1	performance					1058:1068	higher performance	1051:1068	higher performance for ethanol production	1051:1091	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	26	from	poor	1160:1163	arg1	fibre					1186:1190	fibre	1186:1190	fibre	1186:1190	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	26	from	poor	1160:1163	arg1	diferulates					1196:1206	diferulates	1196:1206	diferulates	1196:1206	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	1	27	theme	maize	152:156	arg1	by-product					185:194	a profitable by-product	172:194	a profitable by-product for cellulosic ethanol production	172:228	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	1	27	theme	maize	152:156	arg1	stover					158:163	maize stover	152:163	maize stover	152:163	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	2	28	theme	important	372:380	arg1	constraints					388:398	the most important yield constraints	363:398	the most important yield constraints	363:398	However, yield is reduced by pest damages, stem corn borers being one of the most important yield constraints.					
34078286	1	29	theme	whole	243:247	arg1	plant					249:253	the whole plant	239:253	the whole plant	239:253	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	6	30	theme	particular	1458:1467	arg1	application					1469:1479	each particular application	1453:1479	each particular application	1453:1479	CONCLUSIONS Results evidence that there is no maize cell wall ideotype among the tested for optimal performance for various uses, and maize plants should be specifically bred for each particular application.					
34078286	4	31	theme	wall	573:576	arg1	hydroxycinnamates					626:642	hydroxycinnamates	626:642	hydroxycinnamates	626:642	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	31	theme	wall	573:576	arg1	lignin					615:620	lignin	615:620	lignin	615:620	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	31	theme	wall	573:576	arg1	hemicellulose					600:612	hemicellulose	600:612	hemicellulose	600:612	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	31	theme	wall	573:576	arg1	cellulose					589:597	cellulose	589:597	cellulose	589:597	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	31	theme	wall	573:576	arg1	fractions					578:586	the four cell wall fractions	559:586	the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates)	559:643	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	5	32	theme	low	889:891	arg1	acid					904:907	low p-coumaric acid	889:907	low p-coumaric acid	889:907	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	4	33	theme	maize	656:660	arg1	genotypes					662:670	diverse maize genotypes	648:670	diverse maize genotypes	648:670	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	5	34	dep	RESULTS	783:789	arg1	results					805:811	The following results	791:811	RESULTS The following results	783:811	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	0	35	theme	multifunctional	16:30	arg1	role					32:35	the multifunctional role	12:35	the multifunctional role of the cell wall components in the maize exploitation	12:89	Elucidating the multifunctional role of the cell wall components in the maize exploitation.					
34078286	2	36	theme	pest	319:322	arg1	damages					324:330	pest damages	319:330	pest damages	319:330	However, yield is reduced by pest damages, stem corn borers being one of the most important yield constraints.					
34078286	4	37	theme	diverse	648:654	arg1	genotypes					662:670	diverse maize genotypes	648:670	diverse maize genotypes	648:670	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	38	from	composition	544:554	arg1	genotypes					662:670	diverse maize genotypes	648:670	diverse maize genotypes	648:670	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	1	39	theme	grain	128:132	arg1	use					115:117	the use	111:117	the use of maize grain as food and feed	111:149	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	5	40	theme	ethanol	1074:1080	arg1	production					1082:1091	ethanol production	1074:1091	ethanol production	1074:1091	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	41	theme	inbred	945:950	arg1	lines					952:956	inbred lines	945:956	inbred lines	945:956	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	42	from	fibre	1186:1190	arg1	poor					1160:1163	poor	1160:1163	poor	1160:1163	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	6	43	theme	optimal	1366:1372	arg1	performance					1374:1384	optimal performance	1366:1384	optimal performance for various uses	1366:1401	CONCLUSIONS Results evidence that there is no maize cell wall ideotype among the tested for optimal performance for various uses, and maize plants should be specifically bred for each particular application.					
34078286	5	44	from	richer	1252:1257	arg1	subunits					1264:1271	G subunits	1262:1271	G subunits	1262:1271	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	4	45	theme	cell	568:571	arg1	hydroxycinnamates					626:642	hydroxycinnamates	626:642	hydroxycinnamates	626:642	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	45	theme	cell	568:571	arg1	lignin					615:620	lignin	615:620	lignin	615:620	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	45	theme	cell	568:571	arg1	hemicellulose					600:612	hemicellulose	600:612	hemicellulose	600:612	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	45	theme	cell	568:571	arg1	cellulose					589:597	cellulose	589:597	cellulose	589:597	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	45	theme	cell	568:571	arg1	fractions					578:586	the four cell wall fractions	559:586	the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates)	559:643	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	5	46	theme	diferulate	1014:1023	arg1	cross-linking					1025:1037	high diferulate cross-linking	1009:1037	high diferulate cross-linking	1009:1037	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	1	47	dep	by-product	185:194	arg1	whereas					231:237	whereas	231:237	whereas	231:237	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	5	48	theme	richer	1252:1257	arg1	composition					1240:1250	a lignin polymer composition	1223:1250	a lignin polymer composition richer in G subunits	1223:1271	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	1	49	theme	profitable	174:183	arg1	by-product					185:194	a profitable by-product	172:194	a profitable by-product for cellulosic ethanol production	172:228	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	1	49	theme	profitable	174:183	arg1	stover					158:163	maize stover	152:163	maize stover	152:163	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	0	50	theme	wall	49:52	arg1	components					54:63	the cell wall components	40:63	the cell wall components	40:63	Elucidating the multifunctional role of the cell wall components in the maize exploitation.					
34078286	5	51	theme	following	795:803	arg1	results					805:811	The following results	791:811	RESULTS The following results	783:811	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	3	52	theme	pest	495:498	arg1	resistance					500:509	pest resistance	495:509	pest resistance	495:509	Overall, cell wall composition is key in determining the quality of maize biomass, as well as pest resistance.					
34078286	2	53	theme	constraints	388:398	arg1	one					356:358	one	356:358	one	356:358	However, yield is reduced by pest damages, stem corn borers being one of the most important yield constraints.					
34078286	2	53	theme	constraints	388:398	arg1	constraints					388:398	the most important yield constraints	363:398	the most important yield constraints	363:398	However, yield is reduced by pest damages, stem corn borers being one of the most important yield constraints.					
34078286	1	54	theme	silage	271:276	arg1	production					278:287	silage production	271:287	silage production	271:287	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	5	55	theme	cellulose	987:995	arg1	content					997:1003	high cellulose content	982:1003	high cellulose content	982:1003	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	56	theme	hemicellulose	917:929	arg1	content					931:937	low hemicellulose content	913:937	low hemicellulose content	913:937	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	57	theme	G	1262:1262	arg1	subunits					1264:1271	G subunits	1262:1271	G subunits	1262:1271	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	58	theme	cell	1149:1152	arg1	walls					1154:1158	cell walls	1149:1158	cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits	1149:1271	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	59	contain	have	1144:1147	arg2	walls					1154:1158	cell walls	1149:1158	cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits	1149:1271	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	59	contain	have	1144:1147	arg1	inbreds					1104:1110	inbreds	1104:1110	inbreds with enhanced digestibility	1104:1138	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	4	60	dep	fractions	578:586	arg1	fractions					578:586	the four cell wall fractions	559:586	the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates)	559:643	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	60	dep	fractions	578:586	arg1	lignin					615:620	lignin	615:620	lignin	615:620	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	60	dep	fractions	578:586	arg1	hemicellulose					600:612	hemicellulose	600:612	hemicellulose	600:612	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	60	dep	fractions	578:586	arg1	cellulose					589:597	cellulose	589:597	cellulose	589:597	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	4	60	dep	fractions	578:586	arg1	hydroxycinnamates					626:642	hydroxycinnamates	626:642	hydroxycinnamates	626:642	This study aims to evaluate the composition of the four cell wall fractions (cellulose, hemicellulose, lignin and hydroxycinnamates) in diverse maize genotypes and to understand how this composition influences the resistance to pests, ethanol capacity and digestibility.					
34078286	3	61	theme	cell	410:413	arg1	composition					420:430	cell wall composition	410:430	cell wall composition	410:430	Overall, cell wall composition is key in determining the quality of maize biomass, as well as pest resistance.					
34078286	3	62	theme	maize	469:473	arg1	biomass					475:481	maize biomass	469:481	maize biomass	469:481	Overall, cell wall composition is key in determining the quality of maize biomass, as well as pest resistance.					
34078286	5	63	with	inbreds	1104:1110	arg1	digestibility					1126:1138	enhanced digestibility	1117:1138	enhanced digestibility	1117:1138	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	1	64	theme	maize	122:126	arg1	grain					128:132	maize grain	122:132	maize grain	122:132	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	5	65	theme	poor	1160:1163	arg1	walls					1154:1158	cell walls	1149:1158	cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits	1149:1271	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	6	66	theme	maize	1320:1324	arg1	ideotype					1336:1343	no maize cell wall ideotype	1317:1343	no maize cell wall ideotype	1317:1343	CONCLUSIONS Results evidence that there is no maize cell wall ideotype among the tested for optimal performance for various uses, and maize plants should be specifically bred for each particular application.					
34078286	5	67	theme	high	1009:1012	arg1	cross-linking					1025:1037	high diferulate cross-linking	1009:1037	high diferulate cross-linking	1009:1037	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	68	theme	lignin	1225:1230	arg1	composition					1240:1250	a lignin polymer composition	1223:1250	a lignin polymer composition richer in G subunits	1223:1271	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	1	69	dep	BACKGROUND	92:101	arg1	use					115:117	the use	111:117	the use of maize grain as food and feed	111:149	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	5	70	with	walls	971:975	arg1	content					997:1003	high cellulose content	982:1003	high cellulose content	982:1003	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	70	with	walls	971:975	arg1	cross-linking					1025:1037	high diferulate cross-linking	1009:1037	high diferulate cross-linking	1009:1037	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	1	71	theme	cellulosic	200:209	arg1	production					219:228	cellulosic ethanol production	200:228	cellulosic ethanol production	200:228	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
34078286	0	72	theme	components	54:63	arg1	role					32:35	the multifunctional role	12:35	the multifunctional role of the cell wall components in the maize exploitation	12:89	Elucidating the multifunctional role of the cell wall components in the maize exploitation.					
34078286	0	73	from	role	32:35	arg1	exploitation					78:89	the maize exploitation	68:89	the maize exploitation	68:89	Elucidating the multifunctional role of the cell wall components in the maize exploitation.					
34078286	5	74	theme	p-coumaric	893:902	arg1	acid					904:907	low p-coumaric acid	889:907	low p-coumaric acid	889:907	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	75	theme	polymer	1232:1238	arg1	composition					1240:1250	a lignin polymer composition	1223:1250	a lignin polymer composition richer in G subunits	1223:1271	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	5	76	theme	enhanced	1117:1124	arg1	digestibility					1126:1138	enhanced digestibility	1117:1138	enhanced digestibility	1117:1138	RESULTS The following results can be highlighted: (i) pests' resistant materials may show cell walls with low p-coumaric acid and low hemicellulose content; (ii) inbred lines showing cell walls with high cellulose content and high diferulate cross-linking may present higher performance for ethanol production; (iii) and inbreds with enhanced digestibility may have cell walls poor in neutral detergent fibre and diferulates, combined with a lignin polymer composition richer in G subunits.					
34078286	1	77	theme	ethanol	211:217	arg1	production					219:228	cellulosic ethanol production	200:228	cellulosic ethanol production	200:228	BACKGROUND Besides the use of maize grain as food and feed, maize stover can be a profitable by-product for cellulosic ethanol production, whereas the whole plant can be used for silage production.					
33812192	0	0	theme	red	63:65	arg1	composition					87:97	red wine polysaccharide composition	63:97	red wine polysaccharide composition	63:97	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	7	1	theme	MP	1050:1051	arg1	content					1053:1059	the MP content	1046:1059	the MP content between sonicated and control wines	1046:1095	No significant differences were obtained in the MP content between sonicated and control wines.					
33812192	3	2	theme	non-treated	446:456	arg1	wines					458:462	non-treated wines	446:462	non-treated wines	446:462	A long pomace maceration time was also tested for non-treated wines.					
33812192	6	3	dep	rhamnogalacturonans	852:870	arg1	type					872:875	type II	872:878	type II	872:878	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	6	3	dep	rhamnogalacturonans	852:870	arg1	content					889:895	PRAG content	884:895	PRAG content	884:895	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	6	3	dep	rhamnogalacturonans	852:870	arg1	rhamnogalacturonans					852:870	higher rhamnogalacturonans type II and PRAG content	845:895	higher rhamnogalacturonans type II and PRAG content	845:895	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	1	4	theme	high-power	226:235	arg1	ultrasound					237:246	high-power ultrasound	226:246	high-power ultrasound (US) at 20 and 28 kHz	226:268	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	4	5	from	rich	560:563	arg1	PRAG					593:596	PRAG	593:596	PRAG	593:596	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	4	5	from	rich	560:563	arg1	galactose					582:590	galactose	582:590	galactose (PRAG)	582:597	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	4	5	from	rich	560:563	arg1	arabinose					568:576	arabinose	568:576	arabinose	568:576	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	6	6	theme	mid	826:828	arg1	maceration					830:839	mid maceration	826:839	mid maceration	826:839	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	1	7	theme	molecular	137:145	arg1	weight					147:152	the molecular weight	133:152	the molecular weight	133:152	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	1	8	theme	ultrasound	237:246	arg1	application					211:221	the application	207:221	the application of high-power ultrasound (US) at 20 and 28 kHz	207:268	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	0	9	theme	polysaccharide	72:85	arg1	composition					87:97	red wine polysaccharide composition	63:97	red wine polysaccharide composition	63:97	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	4	10	dep	must	468:471	arg1	samples					473:479	samples	473:479	samples	473:479	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	1	11	from	28 kHz	263:268	arg1	application					211:221	the application	207:221	the application of high-power ultrasound (US) at 20 and 28 kHz	207:268	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	1	11	from	28 kHz	263:268	arg1	ultrasound					237:246	high-power ultrasound	226:246	high-power ultrasound (US) at 20 and 28 kHz	226:268	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	2	12	dep	times	321:325	arg1	mid					338:340	mid	338:340	mid	338:340	Two different pomace maceration times (short and mid) were tested for sonicated and control vinifications.					
33812192	2	12	dep	times	321:325	arg1	short					328:332	short	328:332	short	328:332	Two different pomace maceration times (short and mid) were tested for sonicated and control vinifications.					
33812192	4	13	theme	polysaccharides	544:558	arg1	weight					626:631	the average molecular weight	604:631	the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans	604:678	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	4	13	theme	polysaccharides	544:558	arg1	content					513:519	the content	509:519	the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG)	509:597	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	0	14	theme	wine	67:70	arg1	composition					87:97	red wine polysaccharide composition	63:97	red wine polysaccharide composition	63:97	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	6	15	dep	content	960:966	arg1	closer					919:924	closer	919:924	closer	919:924	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	1	16	from	effect	123:128	arg1	weight					147:152	the molecular weight	133:152	the molecular weight	133:152	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	1	16	from	effect	123:128	arg1	composition					173:183	polysaccharide composition	158:183	polysaccharide composition	158:183	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	1	16	from	effect	123:128	arg1	grapes					281:286	crushed grapes	273:286	crushed grapes	273:286	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	4	17	theme	smaller	636:642	arg1	PRAG					644:647	smaller PRAG	636:647	smaller PRAG	636:647	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	0	18	from	Effect	40:45	arg1	the					50:52	the	50:52	the	50:52	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	1	19	from	20	256:257	arg1	application					211:221	the application	207:221	the application of high-power ultrasound (US) at 20 and 28 kHz	207:268	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	1	19	from	20	256:257	arg1	ultrasound					237:246	high-power ultrasound	226:246	high-power ultrasound (US) at 20 and 28 kHz	226:268	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	6	20	theme	long	971:974	arg1	wines					995:999	long maceration control wines	971:999	long maceration control wines	971:999	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	4	21	theme	average	608:614	arg1	weight					626:631	the average molecular weight	604:631	the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans	604:678	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	7	22	dep	sonicated	1069:1077	arg1	wines					1091:1095	wines	1091:1095	wines	1091:1095	No significant differences were obtained in the MP content between sonicated and control wines.					
33812192	4	23	theme	monosaccharides	524:538	arg1	weight					626:631	the average molecular weight	604:631	the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans	604:678	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	4	23	theme	monosaccharides	524:538	arg1	content					513:519	the content	509:519	the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG)	509:597	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	5	24	theme	wine	719:722	arg1	monosaccharides					724:738	most wine monosaccharides	714:738	most wine monosaccharides	714:738	The 28 kHz had a major effect on most wine monosaccharides and grape polysaccharides.					
33812192	5	25	theme	most	714:717	arg1	monosaccharides					724:738	most wine monosaccharides	714:738	most wine monosaccharides	714:738	The 28 kHz had a major effect on most wine monosaccharides and grape polysaccharides.					
33812192	7	26	theme	significant	1005:1015	arg1	differences					1017:1027	No significant differences	1002:1027	No significant differences	1002:1027	No significant differences were obtained in the MP content between sonicated and control wines.					
33812192	1	27	theme	polysaccharide	158:171	arg1	composition					173:183	polysaccharide composition	158:183	polysaccharide composition	158:183	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	0	28	theme	Ultrasound	0:9	arg1	treatment					11:19	Ultrasound treatment	0:19	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.	0:98	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	6	29	contain	had	841:843	arg2	content					889:895	PRAG content	884:895	PRAG content	884:895	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	6	29	contain	had	841:843	arg2	rhamnogalacturonans					852:870	higher rhamnogalacturonans type II and PRAG content	845:895	higher rhamnogalacturonans type II and PRAG content	845:895	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	6	29	contain	had	841:843	arg1	wine					771:774	The wine	767:774	The wine obtained from sonicated grapes at 28 kHz and with mid maceration	767:839	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	6	29	contain	had	841:843	arg2	type					872:875	type II	872:878	type II	872:878	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	0	30	theme	crushed	24:30	arg1	grapes					32:37	crushed grapes	24:37	crushed grapes	24:37	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	2	31	dep	sonicated	359:367	arg1	vinifications					381:393	vinifications	381:393	vinifications	381:393	Two different pomace maceration times (short and mid) were tested for sonicated and control vinifications.					
33812192	4	32	theme	mannans	672:678	arg1	weight					626:631	the average molecular weight	604:631	the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans	604:678	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	4	32	theme	mannans	672:678	arg1	content					513:519	the content	509:519	the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG)	509:597	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	3	33	theme	maceration	410:419	arg1	time					421:424	A long pomace maceration time	396:424	A long pomace maceration time	396:424	A long pomace maceration time was also tested for non-treated wines.					
33812192	6	34	theme	PRAG	884:887	arg1	content					889:895	PRAG content	884:895	PRAG content	884:895	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	6	34	theme	PRAG	884:887	arg1	rhamnogalacturonans					852:870	higher rhamnogalacturonans type II and PRAG content	845:895	higher rhamnogalacturonans type II and PRAG content	845:895	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	6	35	theme	sonicated	790:798	arg1	grapes					800:805	sonicated grapes	790:805	sonicated grapes	790:805	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	0	36	dep	the	50:52	arg1	must					54:57	must	54:57	must	54:57	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	1	37	theme	musts	188:192	arg1	weight					147:152	the molecular weight	133:152	the molecular weight	133:152	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	1	37	theme	musts	188:192	arg1	composition					173:183	polysaccharide composition	158:183	polysaccharide composition	158:183	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	4	38	theme	PRAG	644:647	arg1	weight					626:631	the average molecular weight	604:631	the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans	604:678	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	4	38	theme	PRAG	644:647	arg1	content					513:519	the content	509:519	the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG)	509:597	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	4	39	from	galactose	582:590	arg1	rich					560:563	rich	560:563	rich	560:563	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	3	40	theme	long	398:401	arg1	time					421:424	A long pomace maceration time	396:424	A long pomace maceration time	396:424	A long pomace maceration time was also tested for non-treated wines.					
33812192	0	41	theme	grapes	32:37	arg1	treatment					11:19	Ultrasound treatment	0:19	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.	0:98	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	5	42	theme	major	698:702	arg1	effect					704:709	a major effect	696:709	a major effect	696:709	The 28 kHz had a major effect on most wine monosaccharides and grape polysaccharides.					
33812192	6	43	theme	polysaccharide	926:939	arg1	content					960:966	polysaccharide and monosaccharide content	926:966	content	960:966	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	1	44	theme	wines	198:202	arg1	weight					147:152	the molecular weight	133:152	the molecular weight	133:152	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	1	44	theme	wines	198:202	arg1	composition					173:183	polysaccharide composition	158:183	polysaccharide composition	158:183	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	1	45	from	application	211:221	arg1	20					256:257	20	256:257	20	256:257	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	1	45	from	application	211:221	arg1	28 kHz					263:268	28 kHz	263:268	28 kHz	263:268	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	3	46	theme	pomace	403:408	arg1	time					421:424	A long pomace maceration time	396:424	A long pomace maceration time	396:424	A long pomace maceration time was also tested for non-treated wines.					
33812192	2	47	theme	pomace	303:308	arg1	times					321:325	Two different pomace maceration times	289:325	Two different pomace maceration times (short and mid)	289:341	Two different pomace maceration times (short and mid) were tested for sonicated and control vinifications.					
33812192	1	48	theme	crushed	273:279	arg1	grapes					281:286	crushed grapes	273:286	crushed grapes	273:286	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	6	49	theme	control	987:993	arg1	wines					995:999	long maceration control wines	971:999	long maceration control wines	971:999	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	2	50	theme	maceration	310:319	arg1	times					321:325	Two different pomace maceration times	289:325	Two different pomace maceration times (short and mid)	289:341	Two different pomace maceration times (short and mid) were tested for sonicated and control vinifications.					
33812192	2	51	theme	different	293:301	arg1	times					321:325	Two different pomace maceration times	289:325	Two different pomace maceration times (short and mid)	289:341	Two different pomace maceration times (short and mid) were tested for sonicated and control vinifications.					
33812192	6	52	theme	higher	845:850	arg1	type					872:875	type II	872:878	type II	872:878	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	6	52	theme	higher	845:850	arg1	content					889:895	PRAG content	884:895	PRAG content	884:895	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	6	52	theme	higher	845:850	arg1	rhamnogalacturonans					852:870	higher rhamnogalacturonans type II and PRAG content	845:895	higher rhamnogalacturonans type II and PRAG content	845:895	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	0	53	from	composition	87:97	arg1	the					50:52	the	50:52	the	50:52	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	6	54	theme	monosaccharide	945:958	arg1	content					960:966	polysaccharide and monosaccharide content	926:966	content	960:966	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	6	55	theme	maceration	976:985	arg1	wines					995:999	long maceration control wines	971:999	long maceration control wines	971:999	The wine obtained from sonicated grapes at 28 kHz and with mid maceration had higher rhamnogalacturonans type II and PRAG content than its control, and closer polysaccharide and monosaccharide content to long maceration control wines.					
33812192	4	56	theme	mannoproteins	650:662	arg1	weight					626:631	the average molecular weight	604:631	the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans	604:678	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	4	56	theme	mannoproteins	650:662	arg1	content					513:519	the content	509:519	the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG)	509:597	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	4	57	theme	rich	560:563	arg1	monosaccharides					524:538	monosaccharides	524:538	monosaccharides	524:538	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	1	58	dep	ultrasound	237:246	arg1	US					249:250	US	249:250	US	249:250	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	0	59	dep	treatment	11:19	arg1	composition					87:97	red wine polysaccharide composition	63:97	red wine polysaccharide composition	63:97	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	0	59	dep	treatment	11:19	arg1	Effect					40:45	Effect	40:45	Effect on the must	40:57	Ultrasound treatment of crushed grapes: Effect on the must and red wine polysaccharide composition.					
33812192	5	60	theme	grape	744:748	arg1	polysaccharides					750:764	grape polysaccharides	744:764	grape polysaccharides	744:764	The 28 kHz had a major effect on most wine monosaccharides and grape polysaccharides.					
33812192	1	61	theme	application	211:221	arg1	musts					188:192	musts	188:192	musts	188:192	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	1	61	theme	application	211:221	arg1	wines					198:202	wines	198:202	wines	198:202	This paper studied the effect on the molecular weight and polysaccharide composition of musts and wines of the application of high-power ultrasound (US) at 20 and 28 kHz on crushed grapes.					
33812192	4	62	from	arabinose	568:576	arg1	rich					560:563	rich	560:563	rich	560:563	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
33812192	5	63	contain	had	692:694	arg1	28 kHz					685:690	The 28 kHz	681:690	The 28 kHz	681:690	The 28 kHz had a major effect on most wine monosaccharides and grape polysaccharides.					
33812192	5	63	contain	had	692:694	arg2	effect					704:709	a major effect	696:709	a major effect	696:709	The 28 kHz had a major effect on most wine monosaccharides and grape polysaccharides.					
33812192	4	64	theme	molecular	616:624	arg1	weight					626:631	the average molecular weight	604:631	the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans	604:678	In must samples, US significantly increased the content of monosaccharides and polysaccharides rich in arabinose and galactose (PRAG), and the average molecular weight of smaller PRAG, mannoproteins (MP) or mannans.					
34702464	5	0	used	used	564:567	arg2	CME					553:555	CME	553:555	CME	553:555	CME can be used to produce BC.					
34702464	8	1	theme	significant	936:946	arg1	changes					948:954	no significant changes	933:954	no significant changes in yields and production rates	933:985	BC production was scaled up from 30 mL to 15 L, using lab-scale experiments conditions, with no significant changes in yields and production rates, suggesting a robust process with industrial possibilities.					
34702464	1	2	dep	Worldwide	89:97	arg1	%					105:105	only 8%	99:105	Worldwide only 8% of the biomass from harvested cacao fruits	89:148	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	6	3	dep	G.	668:669	arg1	xylinus					671:677	xylinus	671:677	xylinus	671:677	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	8	4	theme	experiments	904:914	arg1	conditions					916:925	lab-scale experiments conditions	894:925	lab-scale experiments conditions	894:925	BC production was scaled up from 30 mL to 15 L, using lab-scale experiments conditions, with no significant changes in yields and production rates, suggesting a robust process with industrial possibilities.					
34702464	3	5	theme	potential	351:359	arg1	source					368:373	a potential carbon source	349:373	a potential carbon source for cellulose production	349:398	CME's composition and availability suggest a potential carbon source for cellulose production.					
34702464	8	6	from	changes	948:954	arg1	yields					959:964	yields	959:964	yields	959:964	BC production was scaled up from 30 mL to 15 L, using lab-scale experiments conditions, with no significant changes in yields and production rates, suggesting a robust process with industrial possibilities.					
34702464	8	6	from	changes	948:954	arg1	rates					981:985	production rates	970:985	production rates	970:985	BC production was scaled up from 30 mL to 15 L, using lab-scale experiments conditions, with no significant changes in yields and production rates, suggesting a robust process with industrial possibilities.					
34702464	2	7	theme	nutrient-rich	236:248	arg1	fluid					250:254	a nutrient-rich fluid	234:254	a nutrient-rich fluid	234:254	Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation.					
34702464	2	7	theme	nutrient-rich	236:248	arg1	exudate					219:225	Cacao mucilage exudate	204:225	Cacao mucilage exudate (CME)	204:231	Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation.					
34702464	8	8	theme	lab-scale	894:902	arg1	conditions					916:925	lab-scale experiments conditions	894:925	lab-scale experiments conditions	894:925	BC production was scaled up from 30 mL to 15 L, using lab-scale experiments conditions, with no significant changes in yields and production rates, suggesting a robust process with industrial possibilities.					
34702464	1	9	theme	fruits	143:148	arg1	beans					168:172	cacao beans	162:172	cacao beans	162:172	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	1	9	theme	fruits	143:148	arg1	Worldwide					89:97	Worldwide	89:97	Worldwide only 8% of the biomass from harvested cacao fruits	89:148	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	4	10	theme	Schramm	425:431	arg1	medium					433:438	the Hestrin and Schramm medium	409:438	medium	433:438	CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus.					
34702464	7	11	theme	CME	792:794	arg1	dilution					796:803	CME dilution and addition	792:816	dilution	796:803	BC production increased from 0.55 ± 0.16 g L-1 up to 13.13 ± 1.09 g L-1 after CME dilution and addition of a nitrogen source.					
34702464	0	12	from	available	43:51	arg1	exudate					80:86	residual cacao mucilage exudate	56:86	residual cacao mucilage exudate	56:86	Cellulose biosynthesis using simple sugars available in residual cacao mucilage exudate.					
34702464	6	13	theme	cellulose	696:704	arg1	yields					706:711	cellulose yields	696:711	cellulose yields	696:711	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	6	14	from	pH	621:622	arg1	CME					657:659	CME	657:659	CME	657:659	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	6	15	theme	low	617:619	arg1	pH					621:622	low pH	617:622	low pH	617:622	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	7	16	theme	BC	714:715	arg1	production					717:726	BC production	714:726	BC production	714:726	BC production increased from 0.55 ± 0.16 g L-1 up to 13.13 ± 1.09 g L-1 after CME dilution and addition of a nitrogen source.					
34702464	3	17	theme	cellulose	379:387	arg1	production					389:398	cellulose production	379:398	cellulose production	379:398	CME's composition and availability suggest a potential carbon source for cellulose production.					
34702464	0	18	theme	Cellulose	0:8	arg1	biosynthesis					10:21	Cellulose biosynthesis	0:21	Cellulose biosynthesis	0:21	Cellulose biosynthesis using simple sugars available in residual cacao mucilage exudate.					
34702464	4	19	theme	cellulose	495:503	arg1	production					510:519	bacterial cellulose (BC) production	485:519	bacterial cellulose (BC) production with Gluconacetobacter xylinus	485:550	CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus.					
34702464	8	20	theme	production	970:979	arg1	rates					981:985	production rates	970:985	production rates	970:985	BC production was scaled up from 30 mL to 15 L, using lab-scale experiments conditions, with no significant changes in yields and production rates, suggesting a robust process with industrial possibilities.					
34702464	1	21	theme	cacao	162:166	arg1	Worldwide					89:97	Worldwide	89:97	Worldwide only 8% of the biomass from harvested cacao fruits	89:148	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	1	21	theme	cacao	162:166	arg1	beans					168:172	cacao beans	162:172	cacao beans	162:172	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	0	22	theme	simple	29:34	arg1	sugars					36:41	simple sugars	29:41	simple sugars available in residual cacao mucilage exudate	29:86	Cellulose biosynthesis using simple sugars available in residual cacao mucilage exudate.					
34702464	4	23	theme	bacterial	485:493	arg1	production					510:519	bacterial cellulose (BC) production	485:519	bacterial cellulose (BC) production with Gluconacetobacter xylinus	485:550	CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus.					
34702464	6	24	from	content	608:614	arg1	CME					657:659	CME	657:659	CME	657:659	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	6	25	theme	sugar	602:606	arg1	content					608:614	the high sugar content	593:614	the high sugar content	593:614	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	4	26	theme	BC	506:507	arg1	production					510:519	bacterial cellulose (BC) production	485:519	bacterial cellulose (BC) production with Gluconacetobacter xylinus	485:550	CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus.					
34702464	6	27	theme	high	597:600	arg1	content					608:614	the high sugar content	593:614	the high sugar content	593:614	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	0	28	theme	available	43:51	arg1	sugars					36:41	simple sugars	29:41	simple sugars available in residual cacao mucilage exudate	29:86	Cellulose biosynthesis using simple sugars available in residual cacao mucilage exudate.					
34702464	8	29	theme	robust	1001:1006	arg1	process					1008:1014	a robust process	999:1014	a robust process	999:1014	BC production was scaled up from 30 mL to 15 L, using lab-scale experiments conditions, with no significant changes in yields and production rates, suggesting a robust process with industrial possibilities.					
34702464	0	30	from	exudate	80:86	arg1	available					43:51	available	43:51	available	43:51	Cellulose biosynthesis using simple sugars available in residual cacao mucilage exudate.					
34702464	2	31	theme	mucilage	210:217	arg1	fluid					250:254	a nutrient-rich fluid	234:254	a nutrient-rich fluid	234:254	Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation.					
34702464	2	31	theme	mucilage	210:217	arg1	CME					228:230	CME	228:230	CME	228:230	Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation.					
34702464	2	31	theme	mucilage	210:217	arg1	exudate					219:225	Cacao mucilage exudate	204:225	Cacao mucilage exudate (CME)	204:231	Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation.					
34702464	4	32	used	used	445:448	arg2	CME					401:403	CME	401:403	CME	401:403	CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus.					
34702464	4	32	used	used	445:448	arg2	medium					433:438	the Hestrin and Schramm medium	409:438	medium	433:438	CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus.					
34702464	4	33	theme	growth	468:473	arg1	media					475:479	growth media	468:479	growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus	468:550	CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus.					
34702464	2	34	theme	Cacao	204:208	arg1	fluid					250:254	a nutrient-rich fluid	234:254	a nutrient-rich fluid	234:254	Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation.					
34702464	2	34	theme	Cacao	204:208	arg1	CME					228:230	CME	228:230	CME	228:230	Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation.					
34702464	2	34	theme	Cacao	204:208	arg1	exudate					219:225	Cacao mucilage exudate	204:225	Cacao mucilage exudate (CME)	204:231	Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation.					
34702464	6	35	theme	G.	668:669	arg1	growth					679:684	G. xylinus growth	668:684	G. xylinus growth affecting cellulose yields	668:711	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	1	36	theme	chocolate-based	178:192	arg1	products					194:201	chocolate-based products	178:201	chocolate-based products	178:201	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	0	37	theme	residual	56:63	arg1	exudate					80:86	residual cacao mucilage exudate	56:86	residual cacao mucilage exudate	56:86	Cellulose biosynthesis using simple sugars available in residual cacao mucilage exudate.					
34702464	4	38	theme	Hestrin	413:419	arg1	medium					433:438	the Hestrin and Schramm medium	409:438	medium	433:438	CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus.					
34702464	8	39	theme	BC	840:841	arg1	production					843:852	BC production	840:852	BC production	840:852	BC production was scaled up from 30 mL to 15 L, using lab-scale experiments conditions, with no significant changes in yields and production rates, suggesting a robust process with industrial possibilities.					
34702464	3	40	theme	carbon	361:366	arg1	source					368:373	a potential carbon source	349:373	a potential carbon source for cellulose production	349:398	CME's composition and availability suggest a potential carbon source for cellulose production.					
34702464	6	41	from	sources	646:652	arg1	CME					657:659	CME	657:659	CME	657:659	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	4	42	theme	Gluconacetobacter	526:542	arg1	xylinus					544:550	Gluconacetobacter xylinus	526:550	Gluconacetobacter xylinus	526:550	CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus.					
34702464	2	43	theme	beans	286:290	arg1	fermentation					292:303	cacao beans fermentation	280:303	cacao beans fermentation	280:303	Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation.					
34702464	7	44	theme	source	832:837	arg1	addition					809:816	CME dilution and addition	792:816	addition	809:816	BC production increased from 0.55 ± 0.16 g L-1 up to 13.13 ± 1.09 g L-1 after CME dilution and addition of a nitrogen source.					
34702464	7	44	theme	source	832:837	arg1	dilution					796:803	CME dilution and addition	792:816	dilution	796:803	BC production increased from 0.55 ± 0.16 g L-1 up to 13.13 ± 1.09 g L-1 after CME dilution and addition of a nitrogen source.					
34702464	1	45	theme	biomass	114:120	arg1	fruits					143:148	the biomass from harvested cacao fruits	110:148	the biomass from harvested cacao fruits	110:148	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	8	46	theme	industrial	1021:1030	arg1	possibilities					1032:1044	industrial possibilities	1021:1044	industrial possibilities	1021:1044	BC production was scaled up from 30 mL to 15 L, using lab-scale experiments conditions, with no significant changes in yields and production rates, suggesting a robust process with industrial possibilities.					
34702464	1	47	theme	from	122:125	arg1	fruits					143:148	the biomass from harvested cacao fruits	110:148	the biomass from harvested cacao fruits	110:148	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	2	48	theme	cacao	280:284	arg1	fermentation					292:303	cacao beans fermentation	280:303	cacao beans fermentation	280:303	Cacao mucilage exudate (CME), a nutrient-rich fluid, is usually lost during cacao beans fermentation.					
34702464	0	49	theme	mucilage	71:78	arg1	exudate					80:86	residual cacao mucilage exudate	56:86	residual cacao mucilage exudate	56:86	Cellulose biosynthesis using simple sugars available in residual cacao mucilage exudate.					
34702464	4	50	with	production	510:519	arg1	xylinus					544:550	Gluconacetobacter xylinus	526:550	Gluconacetobacter xylinus	526:550	CME and the Hestrin and Schramm medium were used, and compared, as growth media for bacterial cellulose (BC) production with Gluconacetobacter xylinus.					
34702464	6	51	theme	nitrogen	637:644	arg1	sources					646:652	limited nitrogen sources	629:652	limited nitrogen sources in CME	629:659	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	1	52	theme	harvested	127:135	arg1	fruits					143:148	the biomass from harvested cacao fruits	110:148	the biomass from harvested cacao fruits	110:148	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	7	53	theme	nitrogen	823:830	arg1	source					832:837	a nitrogen source	821:837	a nitrogen source	821:837	BC production increased from 0.55 ± 0.16 g L-1 up to 13.13 ± 1.09 g L-1 after CME dilution and addition of a nitrogen source.					
34702464	0	54	theme	cacao	65:69	arg1	exudate					80:86	residual cacao mucilage exudate	56:86	residual cacao mucilage exudate	56:86	Cellulose biosynthesis using simple sugars available in residual cacao mucilage exudate.					
34702464	1	55	used	used	153:156	arg2	Worldwide					89:97	Worldwide	89:97	Worldwide only 8% of the biomass from harvested cacao fruits	89:148	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	1	55	used	used	153:156	arg2	beans					168:172	cacao beans	162:172	cacao beans	162:172	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
34702464	6	56	theme	limited	629:635	arg1	sources					646:652	limited nitrogen sources	629:652	limited nitrogen sources in CME	629:659	However, the high sugar content, low pH, and limited nitrogen sources in CME hinder G. xylinus growth affecting cellulose yields.					
34702464	1	57	theme	cacao	137:141	arg1	fruits					143:148	the biomass from harvested cacao fruits	110:148	the biomass from harvested cacao fruits	110:148	Worldwide only 8% of the biomass from harvested cacao fruits is used, as cacao beans, in chocolate-based products.					
32718596	0	0	theme	liquid	81:86	arg1	spectrometry					121:132	liquid chromatography-electrospray mass spectrometry	81:132	liquid chromatography-electrospray mass spectrometry	81:132	A new application of acetylation for analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry.					
32718596	6	1	theme	monosaccharide	1327:1340	arg1	compositions					1342:1353	the monosaccharide compositions	1323:1353	the monosaccharide compositions of plant polysaccharides	1323:1378	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	6	2	used	used	1286:1289	arg2	UHPLC-ESI-MS					1182:1193	The established UHPLC-ESI-MS and QAMS approach	1166:1211	UHPLC-ESI-MS	1182:1193	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	1	3	theme	spectrometry	206:217	arg1	method					234:239	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method	135:239	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method	135:239	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	4	4	theme	relative	951:958	arg1	factor					971:976	a relative correction factor	949:976	a relative correction factor	949:976	Furthermore, quantitative analysis of the multi-monosaccharaides by single marker (QAMS) was developed and validated using a relative correction factor.					
32718596	1	5	theme	monosaccharaides	307:322	arg1	determination					271:283	simultaneous determination	258:283	simultaneous determination of neutral and acidic monosaccharaides	258:322	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	2	6	theme	adducts	552:558	arg1	monitoring					525:534	selective ion monitoring	511:534	selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA)	511:655	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	6	theme	adducts	552:558	arg1	SIM					537:539	SIM	537:539	SIM	537:539	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	7	dep	399	591:593	arg1	Fuc					604:606	Fuc	604:606	Fuc	604:606	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	7	dep	399	591:593	arg1	Rha					596:598	Rha	596:598	Rha	596:598	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	0	8	theme	mass	116:119	arg1	spectrometry					121:132	liquid chromatography-electrospray mass spectrometry	81:132	liquid chromatography-electrospray mass spectrometry	81:132	A new application of acetylation for analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry.					
32718596	2	9	from	monitoring	525:534	arg1	m/z					563:565	m/z 385	563:569	m/z 385 (Ara, Xyl and Rib)	563:588	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	9	from	monitoring	525:534	arg1	443					610:612	443	610:612	443	610:612	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	9	from	monitoring	525:534	arg1	457					637:639	457	637:639	457	637:639	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	9	from	monitoring	525:534	arg1	399					591:593	399	591:593	399	591:593	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	3	10	theme	mobile	775:780	arg1	water					762:766	acetonitrile water	749:766	acetonitrile water	749:766	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	3	10	theme	mobile	775:780	arg1	phase					782:786	the mobile phase	771:786	the mobile phase without additional buffer salt added	771:823	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	5	11	theme	Ephedra	1150:1156	arg1	sinica					1158:1163	Ephedra sinica	1150:1163	Ephedra sinica	1150:1163	The UHPLC-ESI-MS and QAMS were compared and successfully applied to analyze the monosaccharide contents of a purified acidic heteropolysaccharide previously isolated from Ephedra sinica.					
32718596	0	12	theme	chromatography-electrospray	88:114	arg1	spectrometry					121:132	liquid chromatography-electrospray mass spectrometry	81:132	liquid chromatography-electrospray mass spectrometry	81:132	A new application of acetylation for analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry.					
32718596	6	13	theme	high	1223:1226	arg1	precision					1228:1236	high precision	1223:1236	high precision	1223:1236	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	1	14	theme	acetylation	337:347	arg1	derivatization					349:362	acetylation derivatization	337:362	acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses	337:440	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	4	15	theme	multi-monosaccharaides	868:889	arg1	analysis					852:859	quantitative analysis	839:859	quantitative analysis of the multi-monosaccharaides by single marker (QAMS)	839:913	Furthermore, quantitative analysis of the multi-monosaccharaides by single marker (QAMS) was developed and validated using a relative correction factor.					
32718596	3	16	theme	mm	709:710	arg1	C18+					699:702	a Cortecs C18+	689:702	a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm)	689:728	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	3	16	theme	mm	709:710	arg1	μm					726:727	2.1 mm × 150 mm, 1.6 μm	705:727	μm	726:727	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	6	17	theme	high-throughput	1295:1309	arg1	analysis					1311:1318	high-throughput analysis	1295:1318	high-throughput analysis of the monosaccharide compositions of plant polysaccharides	1295:1378	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	0	18	theme	new	2:4	arg1	application					6:16	A new application	0:16	A new application of acetylation for analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry.	0:133	A new application of acetylation for analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry.					
32718596	2	19	with	mode	501:504	arg1	monitoring					525:534	selective ion monitoring	511:534	selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA)	511:655	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	19	with	mode	501:504	arg1	SIM					537:539	SIM	537:539	SIM	537:539	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	6	20	theme	QAMS	1199:1202	arg1	approach					1204:1211	The established UHPLC-ESI-MS and QAMS approach	1166:1211	approach	1204:1211	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	4	21	theme	single	894:899	arg1	QAMS					909:912	QAMS	909:912	QAMS	909:912	Furthermore, quantitative analysis of the multi-monosaccharaides by single marker (QAMS) was developed and validated using a relative correction factor.					
32718596	4	21	theme	single	894:899	arg1	marker					901:906	single marker	894:906	single marker (QAMS)	894:913	Furthermore, quantitative analysis of the multi-monosaccharaides by single marker (QAMS) was developed and validated using a relative correction factor.					
32718596	3	22	theme	buffer	807:812	arg1	salt					814:817	additional buffer salt	796:817	additional buffer salt added	796:823	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	6	23	theme	polysaccharides	1364:1378	arg1	compositions					1342:1353	the monosaccharide compositions	1323:1353	the monosaccharide compositions of plant polysaccharides	1323:1378	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	5	24	theme	purified	1088:1095	arg1	heteropolysaccharide					1104:1123	a purified acidic heteropolysaccharide	1086:1123	a purified acidic heteropolysaccharide previously isolated from Ephedra sinica	1086:1163	The UHPLC-ESI-MS and QAMS were compared and successfully applied to analyze the monosaccharide contents of a purified acidic heteropolysaccharide previously isolated from Ephedra sinica.					
32718596	2	25	theme	ion	521:523	arg1	monitoring					525:534	selective ion monitoring	511:534	selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA)	511:655	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	25	theme	ion	521:523	arg1	SIM					537:539	SIM	537:539	SIM	537:539	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	5	26	theme	acidic	1097:1102	arg1	heteropolysaccharide					1104:1123	a purified acidic heteropolysaccharide	1086:1123	a purified acidic heteropolysaccharide previously isolated from Ephedra sinica	1086:1163	The UHPLC-ESI-MS and QAMS were compared and successfully applied to analyze the monosaccharide contents of a purified acidic heteropolysaccharide previously isolated from Ephedra sinica.					
32718596	2	27	theme	selective	511:519	arg1	monitoring					525:534	selective ion monitoring	511:534	selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA)	511:655	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	27	theme	selective	511:519	arg1	SIM					537:539	SIM	537:539	SIM	537:539	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	5	28	theme	heteropolysaccharide	1104:1123	arg1	contents					1074:1081	the monosaccharide contents	1055:1081	the monosaccharide contents of a purified acidic heteropolysaccharide previously isolated from Ephedra sinica	1055:1163	The UHPLC-ESI-MS and QAMS were compared and successfully applied to analyze the monosaccharide contents of a purified acidic heteropolysaccharide previously isolated from Ephedra sinica.					
32718596	2	29	theme	sodium	545:550	arg1	adducts					552:558	sodium adducts	545:558	sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA)	545:655	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	3	30	theme	Cortecs	691:697	arg1	C18+					699:702	a Cortecs C18+	689:702	a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm)	689:728	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	3	30	theme	Cortecs	691:697	arg1	μm					726:727	2.1 mm × 150 mm, 1.6 μm	705:727	μm	726:727	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	4	31	theme	quantitative	839:850	arg1	analysis					852:859	quantitative analysis	839:859	quantitative analysis of the multi-monosaccharaides by single marker (QAMS)	839:913	Furthermore, quantitative analysis of the multi-monosaccharaides by single marker (QAMS) was developed and validated using a relative correction factor.					
32718596	1	32	theme	simultaneous	258:269	arg1	determination					271:283	simultaneous determination	258:283	simultaneous determination of neutral and acidic monosaccharaides	258:322	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	6	33	contain	possesses	1213:1221	arg2	stability					1239:1247	stability	1239:1247	stability	1239:1247	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	6	33	contain	possesses	1213:1221	arg1	approach					1204:1211	The established UHPLC-ESI-MS and QAMS approach	1166:1211	approach	1204:1211	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	6	33	contain	possesses	1213:1221	arg2	precision					1228:1236	high precision	1223:1236	high precision	1223:1236	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	6	33	contain	possesses	1213:1221	arg2	repeatability					1253:1265	repeatability	1253:1265	repeatability	1253:1265	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	6	33	contain	possesses	1213:1221	arg1	UHPLC-ESI-MS					1182:1193	The established UHPLC-ESI-MS and QAMS approach	1166:1211	UHPLC-ESI-MS	1182:1193	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	2	34	theme	ionization	490:499	arg1	mode					501:504	a positive ionization mode	479:504	a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA)	479:655	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	0	35	theme	acetylation	21:31	arg1	application					6:16	A new application	0:16	A new application of acetylation for analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry.	0:133	A new application of acetylation for analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry.					
32718596	3	36	theme	×	712:712	arg1	C18+					699:702	a Cortecs C18+	689:702	a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm)	689:728	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	3	36	theme	×	712:712	arg1	μm					726:727	2.1 mm × 150 mm, 1.6 μm	705:727	μm	726:727	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	6	37	theme	established	1170:1180	arg1	UHPLC-ESI-MS					1182:1193	The established UHPLC-ESI-MS and QAMS approach	1166:1211	UHPLC-ESI-MS	1182:1193	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	2	38	theme	positive	481:488	arg1	mode					501:504	a positive ionization mode	479:504	a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA)	479:655	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	39	dep	443	610:612	arg1	Glc					615:617	Glc	615:617	Glc	615:617	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	39	dep	443	610:612	arg1	Man					628:630	Man	628:630	Man	628:630	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	39	dep	443	610:612	arg1	Gal					620:622	Gal	620:622	Gal	620:622	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	1	40	theme	gas	388:390	arg1	GC-MS					426:430	GC-MS	426:430	GC-MS	426:430	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	1	40	theme	gas	388:390	arg1	spectrometry					412:423	gas chromatography-mass spectrometry	388:423	gas chromatography-mass spectrometry (GC-MS) analyses	388:440	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	2	41	from	m/z	563:565	arg1	monitoring					525:534	selective ion monitoring	511:534	selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA)	511:655	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	41	from	m/z	563:565	arg1	adducts					552:558	sodium adducts	545:558	sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA)	545:655	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	41	from	m/z	563:565	arg1	SIM					537:539	SIM	537:539	SIM	537:539	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	6	42	theme	plant	1358:1362	arg1	polysaccharides					1364:1378	plant polysaccharides	1358:1378	plant polysaccharides	1358:1378	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	1	43	theme	chromatography-mass	392:410	arg1	GC-MS					426:430	GC-MS	426:430	GC-MS	426:430	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	1	43	theme	chromatography-mass	392:410	arg1	spectrometry					412:423	gas chromatography-mass spectrometry	388:423	gas chromatography-mass spectrometry (GC-MS) analyses	388:440	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	5	44	theme	monosaccharide	1059:1072	arg1	contents					1074:1081	the monosaccharide contents	1055:1081	the monosaccharide contents of a purified acidic heteropolysaccharide previously isolated from Ephedra sinica	1055:1163	The UHPLC-ESI-MS and QAMS were compared and successfully applied to analyze the monosaccharide contents of a purified acidic heteropolysaccharide previously isolated from Ephedra sinica.					
32718596	1	45	theme	novel	137:141	arg1	method					234:239	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method	135:239	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method	135:239	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	1	46	theme	spectrometry	412:423	arg1	analyses					433:440	gas chromatography-mass spectrometry (GC-MS) analyses	388:440	gas chromatography-mass spectrometry (GC-MS) analyses	388:440	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	3	47	theme	additional	796:805	arg1	salt					814:817	additional buffer salt	796:817	additional buffer salt added	796:823	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	1	48	theme	ultra-high-performance	143:164	arg1	UHPLC-ESI-MS					220:231	UHPLC-ESI-MS	220:231	UHPLC-ESI-MS	220:231	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	1	48	theme	ultra-high-performance	143:164	arg1	spectrometry					206:217	ultra-high-performance liquid chromatography-electrospray mass spectrometry	143:217	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method	135:239	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	2	49	dep	457	637:639	arg1	GalA					651:654	GalA	651:654	GalA	651:654	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	49	dep	457	637:639	arg1	GlcA					642:645	GlcA	642:645	GlcA	642:645	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	50	dep	m/z	563:565	arg1	Ara					572:574	Ara	572:574	Ara	572:574	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	50	dep	m/z	563:565	arg1	Rib					585:587	Rib	585:587	Rib	585:587	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	2	50	dep	m/z	563:565	arg1	Xyl					577:579	Xyl	577:579	Xyl	577:579	The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal and Man) and 457 (GlcA and GalA).					
32718596	5	51	attach	isolated	1136:1143	arg1	sinica					1158:1163	Ephedra sinica	1150:1163	Ephedra sinica	1150:1163	The UHPLC-ESI-MS and QAMS were compared and successfully applied to analyze the monosaccharide contents of a purified acidic heteropolysaccharide previously isolated from Ephedra sinica.					
32718596	5	51	attach	isolated	1136:1143	arg2	heteropolysaccharide					1104:1123	a purified acidic heteropolysaccharide	1086:1123	a purified acidic heteropolysaccharide previously isolated from Ephedra sinica	1086:1163	The UHPLC-ESI-MS and QAMS were compared and successfully applied to analyze the monosaccharide contents of a purified acidic heteropolysaccharide previously isolated from Ephedra sinica.					
32718596	1	52	theme	liquid	166:171	arg1	UHPLC-ESI-MS					220:231	UHPLC-ESI-MS	220:231	UHPLC-ESI-MS	220:231	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	1	52	theme	liquid	166:171	arg1	spectrometry					206:217	ultra-high-performance liquid chromatography-electrospray mass spectrometry	143:217	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method	135:239	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	1	53	theme	neutral	288:294	arg1	monosaccharaides					307:322	neutral and acidic monosaccharaides	288:322	neutral and acidic monosaccharaides	288:322	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	0	54	theme	heteropolysaccharides	56:76	arg1	analysis					37:44	analysis	37:44	analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry	37:132	A new application of acetylation for analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry.					
32718596	6	55	theme	compositions	1342:1353	arg1	analysis					1311:1318	high-throughput analysis	1295:1318	high-throughput analysis of the monosaccharide compositions of plant polysaccharides	1295:1378	The established UHPLC-ESI-MS and QAMS approach possesses high precision, stability and repeatability, and can be widely used for high-throughput analysis of the monosaccharide compositions of plant polysaccharides.					
32718596	1	56	theme	chromatography-electrospray	173:199	arg1	UHPLC-ESI-MS					220:231	UHPLC-ESI-MS	220:231	UHPLC-ESI-MS	220:231	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	1	56	theme	chromatography-electrospray	173:199	arg1	spectrometry					206:217	ultra-high-performance liquid chromatography-electrospray mass spectrometry	143:217	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method	135:239	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	3	57	theme	acetonitrile	749:760	arg1	water					762:766	acetonitrile water	749:766	acetonitrile water	749:766	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	3	57	theme	acetonitrile	749:760	arg1	phase					782:786	the mobile phase	771:786	the mobile phase without additional buffer salt added	771:823	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	3	58	theme	mm	718:719	arg1	C18+					699:702	a Cortecs C18+	689:702	a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm)	689:728	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	3	58	theme	mm	718:719	arg1	μm					726:727	2.1 mm × 150 mm, 1.6 μm	705:727	μm	726:727	The separation was achieved on a Cortecs C18+ (2.1 mm × 150 mm, 1.6 μm) within 8 min using acetonitrile water as the mobile phase without additional buffer salt added.					
32718596	0	59	theme	acidic	49:54	arg1	heteropolysaccharides					56:76	acidic heteropolysaccharides	49:76	acidic heteropolysaccharides	49:76	A new application of acetylation for analysis of acidic heteropolysaccharides by liquid chromatography-electrospray mass spectrometry.					
32718596	1	60	theme	mass	201:204	arg1	UHPLC-ESI-MS					220:231	UHPLC-ESI-MS	220:231	UHPLC-ESI-MS	220:231	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	1	60	theme	mass	201:204	arg1	spectrometry					206:217	ultra-high-performance liquid chromatography-electrospray mass spectrometry	143:217	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method	135:239	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
32718596	4	61	theme	correction	960:969	arg1	factor					971:976	a relative correction factor	949:976	a relative correction factor	949:976	Furthermore, quantitative analysis of the multi-monosaccharaides by single marker (QAMS) was developed and validated using a relative correction factor.					
32718596	1	62	theme	acidic	300:305	arg1	monosaccharaides					307:322	neutral and acidic monosaccharaides	288:322	neutral and acidic monosaccharaides	288:322	A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses.					
33039534	0	0	theme	soluble	85:91	arg1	carboxymethyl					114:126	okara soluble dietary fiber/sodium carboxymethyl	79:126	okara soluble dietary fiber/sodium carboxymethyl	79:126	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	1	1	theme	essential	335:343	arg1	oil					345:347	thyme essential oil	329:347	thyme essential oil (TEO)	329:353	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	1	1	theme	essential	335:343	arg1	TEO					350:352	TEO	350:352	TEO	350:352	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	9	2	theme	46.33	1326:1330	arg1	%					1331:1331	%	1331:1331	%	1331:1331	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	9	3	theme	scavenging	1307:1316	arg1	rate					1318:1321	a DPPH-free radical scavenging rate	1287:1321	a DPPH-free radical scavenging rate of 46.33% ± 0.72%	1287:1339	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	6	4	theme	SDF/CMC-Na	994:1003	arg1	film					1015:1018	SDF/CMC-Na composite film	994:1018	SDF/CMC-Na composite film	994:1018	The addition of pectin led to significantly higher mechanical and optical properties of the composite film, compared with SDF/CMC-Na composite film.					
33039534	0	5	theme	okara	79:83	arg1	carboxymethyl					114:126	okara soluble dietary fiber/sodium carboxymethyl	79:126	okara soluble dietary fiber/sodium carboxymethyl	79:126	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	6	6	theme	higher	916:921	arg1	properties					946:955	significantly higher mechanical and optical properties	902:955	significantly higher mechanical and optical properties of the composite film	902:977	The addition of pectin led to significantly higher mechanical and optical properties of the composite film, compared with SDF/CMC-Na composite film.					
33039534	10	7	theme	composite	1475:1483	arg1	films					1485:1489	SDF/pectin/CMC-Na composite films	1457:1489	SDF/pectin/CMC-Na composite films	1457:1489	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	3	8	theme	different	498:506	arg1	pectin					519:524	pectin	519:524	pectin	519:524	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	3	8	theme	different	498:506	arg1	amounts					508:514	different amounts	498:514	different amounts of pectin	498:524	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	10	9	theme	enhanced	1496:1503	arg1	properties					1537:1546	enhanced mechanical, optical and barrier properties	1496:1546	properties	1537:1546	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	6	10	dep	led	895:897	arg1	compared					980:987	compared	980:987	compared with SDF/CMC-Na composite film	980:1018	The addition of pectin led to significantly higher mechanical and optical properties of the composite film, compared with SDF/CMC-Na composite film.					
33039534	0	11	theme	fiber/sodium	101:112	arg1	carboxymethyl					114:126	okara soluble dietary fiber/sodium carboxymethyl	79:126	okara soluble dietary fiber/sodium carboxymethyl	79:126	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	0	12	from	Study	0:4	arg1	antioxidant					37:47	antioxidant	37:47	antioxidant	37:47	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	0	12	from	Study	0:4	arg1	activity					67:74	antimicrobial activity	53:74	antimicrobial activity	53:74	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	0	12	from	Study	0:4	arg1	properties					25:34	physicochemical properties	9:34	physicochemical properties	9:34	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	4	13	theme	composite	742:750	arg1	membrane					752:759	the composite membrane	738:759	the composite membrane	738:759	In general, the addition of TEO can improve the properties of the composite membrane.					
33039534	9	14	theme	radical	1299:1305	arg1	rate					1318:1321	a DPPH-free radical scavenging rate	1287:1321	a DPPH-free radical scavenging rate of 46.33% ± 0.72%	1287:1339	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	0	15	theme	dietary	93:99	arg1	carboxymethyl					114:126	okara soluble dietary fiber/sodium carboxymethyl	79:126	okara soluble dietary fiber/sodium carboxymethyl	79:126	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	6	16	theme	pectin	888:893	arg1	addition					876:883	The addition	872:883	The addition of pectin	872:893	The addition of pectin led to significantly higher mechanical and optical properties of the composite film, compared with SDF/CMC-Na composite film.					
33039534	7	17	theme	 ± 0.42	1114:1120	arg1	%					1113:1113	%	1113:1113	%	1113:1113	The tensile strength reached 21.419 ± 2.22 MPa, and the minimum transparency reduced to 88.9% ± 0.42%, with increasing pectin.					
33039534	9	18	theme	antibacterial	1348:1360	arg1	activity					1362:1369	antibacterial activity	1348:1369	antibacterial activity against E. coli and S. aureus bacteria	1348:1408	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	4	19	theme	TEO	704:706	arg1	addition					692:699	the addition	688:699	the addition of TEO	688:706	In general, the addition of TEO can improve the properties of the composite membrane.					
33039534	8	20	theme	water	1161:1165	arg1	resistance					1167:1176	the water resistance	1157:1176	the water resistance	1157:1176	Notably, the water resistance and oil resistance were enhanced.					
33039534	5	21	theme	good	830:833	arg1	interaction					835:845	good interaction	830:845	good interaction with the polymer matrix	830:869	Pectin was homogeneously distributed within the films and exhibited good interaction with the polymer matrix.					
33039534	3	22	theme	pectin	519:524	arg1	pectin					519:524	pectin	519:524	pectin	519:524	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	3	22	theme	pectin	519:524	arg1	amounts					508:514	different amounts	498:514	different amounts of pectin	498:524	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	0	23	theme	carboxymethyl	114:126	arg1	antioxidant					37:47	antioxidant	37:47	antioxidant	37:47	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	0	23	theme	carboxymethyl	114:126	arg1	activity					67:74	antimicrobial activity	53:74	antimicrobial activity	53:74	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	0	23	theme	carboxymethyl	114:126	arg1	properties					25:34	physicochemical properties	9:34	physicochemical properties	9:34	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	7	24	theme	88.9	1109:1112	arg1	%					1113:1113	%	1113:1113	%	1113:1113	The tensile strength reached 21.419 ± 2.22 MPa, and the minimum transparency reduced to 88.9% ± 0.42%, with increasing pectin.					
33039534	7	25	theme	minimum	1077:1083	arg1	transparency					1085:1096	the minimum transparency	1073:1096	the minimum transparency	1073:1096	The tensile strength reached 21.419 ± 2.22 MPa, and the minimum transparency reduced to 88.9% ± 0.42%, with increasing pectin.					
33039534	4	26	theme	membrane	752:759	arg1	properties					724:733	the properties	720:733	the properties of the composite membrane	720:759	In general, the addition of TEO can improve the properties of the composite membrane.					
33039534	5	27	theme	polymer	856:862	arg1	matrix					864:869	the polymer matrix	852:869	the polymer matrix	852:869	Pectin was homogeneously distributed within the films and exhibited good interaction with the polymer matrix.					
33039534	9	28	dep	coli	1382:1385	arg1	bacteria					1401:1408	bacteria	1401:1408	bacteria	1401:1408	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	2	29	theme	resources	472:480	arg1	utilization					451:461	the full utilization	442:461	the full utilization of waste resources	442:480	We aimed to exploit biodegradable edible films and realize the full utilization of waste resources.					
33039534	10	30	theme	SDF/pectin/CMC-Na	1457:1473	arg1	films					1485:1489	SDF/pectin/CMC-Na composite films	1457:1489	SDF/pectin/CMC-Na composite films	1457:1489	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	10	31	theme	barrier	1529:1535	arg1	properties					1537:1546	enhanced mechanical, optical and barrier properties	1496:1546	properties	1537:1546	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	1	32	theme	Active	214:219	arg1	films					228:232	Active edible films	214:232	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO)	214:353	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	9	33	theme	 ± 0.72	1332:1338	arg1	%					1331:1331	%	1331:1331	%	1331:1331	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	6	34	theme	film	974:977	arg1	properties					946:955	significantly higher mechanical and optical properties	902:955	significantly higher mechanical and optical properties of the composite film	902:977	The addition of pectin led to significantly higher mechanical and optical properties of the composite film, compared with SDF/CMC-Na composite film.					
33039534	10	35	theme	excellent	1552:1560	arg1	candidates					1562:1571	excellent candidates	1552:1571	excellent candidates for active edible packaging	1552:1599	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	10	35	theme	excellent	1552:1560	arg1	Antioxidant					1427:1437	Antioxidant	1427:1437	Antioxidant	1427:1437	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	10	35	theme	excellent	1552:1560	arg1	antibacterial					1443:1455	antibacterial	1443:1455	antibacterial	1443:1455	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	1	36	theme	edible	221:226	arg1	films					228:232	Active edible films	214:232	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO)	214:353	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	1	37	theme	sodium	286:291	arg1	CMCNa					318:322	CMCNa	318:322	CMCNa	318:322	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	1	37	theme	sodium	286:291	arg1	cellulose					307:315	sodium carboxymethyl cellulose	286:315	sodium carboxymethyl cellulose (CMCNa)	286:323	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	0	38	theme	oil	154:156	arg1	films					182:186	essential oil active edible composite films	144:186	essential oil active edible composite films incorporated with pectin	144:211	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	3	39	from	effects	487:493	arg1	properties					533:542	properties	533:542	properties	533:542	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	3	39	from	effects	487:493	arg1	characterization					559:574	structural characterization	548:574	structural characterization	548:574	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	2	40	theme	edible	417:422	arg1	films					424:428	biodegradable edible films	403:428	biodegradable edible films	403:428	We aimed to exploit biodegradable edible films and realize the full utilization of waste resources.					
33039534	0	41	theme	physicochemical	9:23	arg1	properties					25:34	physicochemical properties	9:34	physicochemical properties	9:34	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	1	42	theme	carboxymethyl	293:305	arg1	CMCNa					318:322	CMCNa	318:322	CMCNa	318:322	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	1	42	theme	carboxymethyl	293:305	arg1	cellulose					307:315	sodium carboxymethyl cellulose	286:315	sodium carboxymethyl cellulose (CMCNa)	286:323	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	0	43	theme	essential	144:152	arg1	films					182:186	essential oil active edible composite films	144:186	essential oil active edible composite films incorporated with pectin	144:211	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	9	44	theme	composite	1216:1224	arg1	films					1226:1230	The composite films	1212:1230	The composite films	1212:1230	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	3	45	theme	structural	548:557	arg1	characterization					559:574	structural characterization	548:574	structural characterization	548:574	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	2	46	theme	biodegradable	403:415	arg1	films					424:428	biodegradable edible films	403:428	biodegradable edible films	403:428	We aimed to exploit biodegradable edible films and realize the full utilization of waste resources.					
33039534	2	47	theme	waste	466:470	arg1	resources					472:480	waste resources	466:480	waste resources	466:480	We aimed to exploit biodegradable edible films and realize the full utilization of waste resources.					
33039534	0	48	theme	edible	165:170	arg1	films					182:186	essential oil active edible composite films	144:186	essential oil active edible composite films incorporated with pectin	144:211	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	3	49	theme	solution	639:646	arg1	method					668:673	a solution casting evaporation method	637:673	a solution casting evaporation method	637:673	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	3	50	theme	film	593:596	arg1	properties					533:542	properties	533:542	properties	533:542	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	3	50	theme	film	593:596	arg1	characterization					559:574	structural characterization	548:574	structural characterization	548:574	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	9	51	theme	%	1339:1339	arg1	rate					1318:1321	a DPPH-free radical scavenging rate	1287:1321	a DPPH-free radical scavenging rate of 46.33% ± 0.72%	1287:1339	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	9	52	theme	DPPH-free	1289:1297	arg1	rate					1318:1321	a DPPH-free radical scavenging rate	1287:1321	a DPPH-free radical scavenging rate of 46.33% ± 0.72%	1287:1339	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	0	53	theme	active	158:163	arg1	films					182:186	essential oil active edible composite films	144:186	essential oil active edible composite films incorporated with pectin	144:211	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	1	54	theme	okara	243:247	arg1	fiber					265:269	okara soluble dietary fiber	243:269	okara soluble dietary fiber (SDF)	243:275	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	1	54	theme	okara	243:247	arg1	SDF					272:274	SDF	272:274	SDF	272:274	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	0	55	theme	antimicrobial	53:65	arg1	activity					67:74	antimicrobial activity	53:74	antimicrobial activity	53:74	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	6	56	theme	optical	938:944	arg1	properties					946:955	significantly higher mechanical and optical properties	902:955	significantly higher mechanical and optical properties of the composite film	902:977	The addition of pectin led to significantly higher mechanical and optical properties of the composite film, compared with SDF/CMC-Na composite film.					
33039534	6	57	theme	composite	964:972	arg1	film					974:977	the composite film	960:977	the composite film	960:977	The addition of pectin led to significantly higher mechanical and optical properties of the composite film, compared with SDF/CMC-Na composite film.					
33039534	2	58	theme	full	446:449	arg1	utilization					451:461	the full utilization	442:461	the full utilization of waste resources	442:480	We aimed to exploit biodegradable edible films and realize the full utilization of waste resources.					
33039534	0	59	theme	composite	172:180	arg1	films					182:186	essential oil active edible composite films	144:186	essential oil active edible composite films incorporated with pectin	144:211	Study on physicochemical properties, antioxidant and antimicrobial activity of okara soluble dietary fiber/sodium carboxymethyl cellulose/thyme essential oil active edible composite films incorporated with pectin.					
33039534	9	60	theme	satisfactory	1247:1258	arg1	activity					1272:1279	satisfactory antioxidant activity	1247:1279	satisfactory antioxidant activity	1247:1279	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	3	61	theme	composite	583:591	arg1	film					593:596	the composite film	579:596	the composite film with or without TEO	579:616	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	10	62	theme	active	1577:1582	arg1	packaging					1591:1599	active edible packaging	1577:1599	active edible packaging	1577:1599	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	9	63	contain	possessed	1237:1245	arg2	activity					1272:1279	satisfactory antioxidant activity	1247:1279	satisfactory antioxidant activity	1247:1279	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	9	63	contain	possessed	1237:1245	arg1	films					1226:1230	The composite films	1212:1230	The composite films	1212:1230	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	6	64	theme	mechanical	923:932	arg1	properties					946:955	significantly higher mechanical and optical properties	902:955	significantly higher mechanical and optical properties of the composite film	902:977	The addition of pectin led to significantly higher mechanical and optical properties of the composite film, compared with SDF/CMC-Na composite film.					
33039534	7	65	theme	tensile	1025:1031	arg1	strength					1033:1040	The tensile strength	1021:1040	The tensile strength	1021:1040	The tensile strength reached 21.419 ± 2.22 MPa, and the minimum transparency reduced to 88.9% ± 0.42%, with increasing pectin.					
33039534	9	66	theme	antioxidant	1260:1270	arg1	activity					1272:1279	satisfactory antioxidant activity	1247:1279	satisfactory antioxidant activity	1247:1279	The composite films also possessed satisfactory antioxidant activity, with a DPPH-free radical scavenging rate of 46.33% ± 0.72%, while antibacterial activity against E. coli and S. aureus bacteria was not obvious.					
33039534	10	67	theme	edible	1584:1589	arg1	packaging					1591:1599	active edible packaging	1577:1599	active edible packaging	1577:1599	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	1	68	theme	soluble	249:255	arg1	fiber					265:269	okara soluble dietary fiber	243:269	okara soluble dietary fiber (SDF)	243:275	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	1	68	theme	soluble	249:255	arg1	SDF					272:274	SDF	272:274	SDF	272:274	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	10	69	theme	optical	1517:1523	arg1	properties					1537:1546	enhanced mechanical, optical and barrier properties	1496:1546	properties	1537:1546	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	3	70	theme	casting	648:654	arg1	method					668:673	a solution casting evaporation method	637:673	a solution casting evaporation method	637:673	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	10	71	dep	Antioxidant	1427:1437	arg1	films					1485:1489	SDF/pectin/CMC-Na composite films	1457:1489	SDF/pectin/CMC-Na composite films	1457:1489	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	3	72	dep	properties	533:542	arg1	the					529:531	the	529:531	the	529:531	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	10	73	with	Antioxidant	1427:1437	arg1	properties					1537:1546	enhanced mechanical, optical and barrier properties	1496:1546	properties	1537:1546	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	1	74	theme	dietary	257:263	arg1	fiber					265:269	okara soluble dietary fiber	243:269	okara soluble dietary fiber (SDF)	243:275	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	1	74	theme	dietary	257:263	arg1	SDF					272:274	SDF	272:274	SDF	272:274	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	10	75	theme	mechanical	1505:1514	arg1	properties					1537:1546	enhanced mechanical, optical and barrier properties	1496:1546	properties	1537:1546	Antioxidant and antibacterial SDF/pectin/CMC-Na composite films with enhanced mechanical, optical and barrier properties are excellent candidates for active edible packaging.					
33039534	3	76	theme	evaporation	656:666	arg1	method					668:673	a solution casting evaporation method	637:673	a solution casting evaporation method	637:673	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	3	77	theme	amounts	508:514	arg1	effects					487:493	The effects	483:493	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO	483:616	The effects of different amounts of pectin on the properties and structural characterization of the composite film with or without TEO were studied using a solution casting evaporation method.					
33039534	5	78	with	interaction	835:845	arg1	matrix					864:869	the polymer matrix	852:869	the polymer matrix	852:869	Pectin was homogeneously distributed within the films and exhibited good interaction with the polymer matrix.					
33039534	1	79	theme	thyme	329:333	arg1	oil					345:347	thyme essential oil	329:347	thyme essential oil (TEO)	329:353	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	1	79	theme	thyme	329:333	arg1	TEO					350:352	TEO	350:352	TEO	350:352	Active edible films based on okara soluble dietary fiber (SDF), pectin, sodium carboxymethyl cellulose (CMCNa) and thyme essential oil (TEO) were successfully prepared.					
33039534	8	80	theme	oil	1182:1184	arg1	resistance					1186:1195	oil resistance	1182:1195	oil resistance	1182:1195	Notably, the water resistance and oil resistance were enhanced.					
33039534	6	81	theme	composite	1005:1013	arg1	film					1015:1018	SDF/CMC-Na composite film	994:1018	SDF/CMC-Na composite film	994:1018	The addition of pectin led to significantly higher mechanical and optical properties of the composite film, compared with SDF/CMC-Na composite film.					
32335112	4	0	theme	xyloglucan	568:577	arg1	region					579:584	a xyloglucan region	566:584	a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues	566:670	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	4	0	theme	xyloglucan	568:577	arg1	region					521:526	an arabinan region	509:526	an arabinan region composed of 1,5-linked Araf residues	509:563	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	0	1	from	characterization	11:26	arg1	Yu					103:104	Yu	103:104	Yu	103:104	Structural characterization and immunostimulatory activity of polysaccharides from Pyrus sinkiangensis Yu.					
32335112	3	2	theme	monosaccharide	346:359	arg1	composition					361:371	monosaccharide composition	346:371	monosaccharide composition	346:371	According to the IR, NMR spectra, monosaccharide composition, and methylation analyses, PSNP-1 was mainly composed of glucose and xylose residues, which form a typical xyloglucan.					
32335112	4	3	link	1,5-linked	540:549	arg1	residues					556:563	1,5-linked Araf residues	540:563	1,5-linked Araf residues	540:563	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	2	4	theme	permeation	285:294	arg1	chromatography					296:309	gel permeation chromatography	281:309	gel permeation chromatography	281:309	by using the combined techniques of ion-exchange and gel permeation chromatography.					
32335112	2	5	theme	gel	281:283	arg1	chromatography					296:309	gel permeation chromatography	281:309	gel permeation chromatography	281:309	by using the combined techniques of ion-exchange and gel permeation chromatography.					
32335112	5	6	theme	macrophages	791:801	arg1	secretion					752:760	cytokine secretion	743:760	cytokine secretion	743:760	PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL.					
32335112	5	6	theme	macrophages	791:801	arg1	viability					716:724	cell viability	711:724	cell viability	711:724	PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL.					
32335112	5	6	theme	macrophages	791:801	arg1	release					730:736	NO release	727:736	NO release	727:736	PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL.					
32335112	5	7	theme	cell	711:714	arg1	viability					716:724	cell viability	711:724	cell viability	711:724	PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL.					
32335112	5	8	theme	RAW264.7	782:789	arg1	macrophages					791:801	RAW264.7 macrophages	782:801	RAW264.7 macrophages	782:801	PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL.					
32335112	1	9	dep	PSNP-1	136:141	arg1	PSNP-2					155:160	PSNP-2	155:160	PSNP-2	155:160	Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa) were isolated from the pulp of Pyrus sinkiangensis Yu.					
32335112	3	10	theme	glucose	430:436	arg1	residues					449:456	glucose and xylose residues	430:456	glucose and xylose residues	430:456	According to the IR, NMR spectra, monosaccharide composition, and methylation analyses, PSNP-1 was mainly composed of glucose and xylose residues, which form a typical xyloglucan.					
32335112	2	11	theme	ion-exchange	264:275	arg1	techniques					250:259	the combined techniques	237:259	the combined techniques of ion-exchange and gel permeation chromatography	237:309	by using the combined techniques of ion-exchange and gel permeation chromatography.					
32335112	3	12	theme	methylation	378:388	arg1	analyses					390:397	methylation analyses	378:397	methylation analyses	378:397	According to the IR, NMR spectra, monosaccharide composition, and methylation analyses, PSNP-1 was mainly composed of glucose and xylose residues, which form a typical xyloglucan.					
32335112	0	13	from	activity	50:57	arg1	Yu					103:104	Yu	103:104	Yu	103:104	Structural characterization and immunostimulatory activity of polysaccharides from Pyrus sinkiangensis Yu.					
32335112	0	14	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunostimulatory activity of polysaccharides from Pyrus sinkiangensis Yu.					
32335112	4	15	theme	Araf	551:554	arg1	residues					556:563	1,5-linked Araf residues	540:563	1,5-linked Araf residues	540:563	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	1	16	theme	neutral	111:117	arg1	polysaccharides					119:133	Two neutral polysaccharides	107:133	Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa)	107:171	Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa) were isolated from the pulp of Pyrus sinkiangensis Yu.					
32335112	4	17	theme	1,4-	639:642	arg1	residues					663:670	1,4-, 1,4,6-linked Glcp residues	639:670	1,4-, 1,4,6-linked Glcp residues	639:670	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	0	18	theme	immunostimulatory	32:48	arg1	activity					50:57	immunostimulatory activity	32:57	immunostimulatory activity	32:57	Structural characterization and immunostimulatory activity of polysaccharides from Pyrus sinkiangensis Yu.					
32335112	4	19	theme	1,5-linked	540:549	arg1	residues					556:563	1,5-linked Araf residues	540:563	1,5-linked Araf residues	540:563	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	1	20	attach	isolated	178:185	arg2	polysaccharides					119:133	Two neutral polysaccharides	107:133	Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa)	107:171	Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa) were isolated from the pulp of Pyrus sinkiangensis Yu.					
32335112	1	20	attach	isolated	178:185	arg1	pulp					196:199	the pulp	192:199	the pulp of Pyrus sinkiangensis Yu	192:225	Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa) were isolated from the pulp of Pyrus sinkiangensis Yu.					
32335112	2	21	theme	chromatography	296:309	arg1	techniques					250:259	the combined techniques	237:259	the combined techniques of ion-exchange and gel permeation chromatography	237:309	by using the combined techniques of ion-exchange and gel permeation chromatography.					
32335112	4	22	contain	contained	499:507	arg1	PSNP-2					492:497	PSNP-2	492:497	PSNP-2	492:497	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	4	22	contain	contained	499:507	arg2	region					521:526	an arabinan region	509:526	an arabinan region composed of 1,5-linked Araf residues	509:563	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	4	22	contain	contained	499:507	arg2	region					579:584	a xyloglucan region	566:584	a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues	566:670	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	0	23	from	Yu	103:104	arg1	polysaccharides					62:76	polysaccharides	62:76	polysaccharides from Pyrus sinkiangensis Yu	62:104	Structural characterization and immunostimulatory activity of polysaccharides from Pyrus sinkiangensis Yu.					
32335112	0	23	from	Yu	103:104	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunostimulatory activity of polysaccharides from Pyrus sinkiangensis Yu.					
32335112	0	23	from	Yu	103:104	arg1	activity					50:57	immunostimulatory activity	32:57	immunostimulatory activity	32:57	Structural characterization and immunostimulatory activity of polysaccharides from Pyrus sinkiangensis Yu.					
32335112	5	24	theme	250 μg/mL	821:829	arg1	adosage					810:816	the adosage	806:816	the adosage of 250 μg/mL	806:829	PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL.					
32335112	3	25	theme	xylose	442:447	arg1	residues					449:456	glucose and xylose residues	430:456	glucose and xylose residues	430:456	According to the IR, NMR spectra, monosaccharide composition, and methylation analyses, PSNP-1 was mainly composed of glucose and xylose residues, which form a typical xyloglucan.					
32335112	4	26	theme	Glcp	658:661	arg1	residues					663:670	1,4-, 1,4,6-linked Glcp residues	639:670	1,4-, 1,4,6-linked Glcp residues	639:670	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	6	27	theme	immunostimulatory	905:921	arg1	activity					923:930	macrophage-mediated immunostimulatory activity	885:930	macrophage-mediated immunostimulatory activity	885:930	It was suggested that PSNP-1 and PSNP-2 may increase macrophage-mediated immunostimulatory activity.					
32335112	5	28	theme	cytokine	743:750	arg1	secretion					752:760	cytokine secretion	743:760	cytokine secretion	743:760	PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL.					
32335112	4	29	theme	1,2-linked	618:627	arg1	Xylp					629:632	1,2-linked Xylp	618:632	1,2-linked Xylp	618:632	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	4	30	theme	arabinan	512:519	arg1	region					579:584	a xyloglucan region	566:584	a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues	566:670	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	4	30	theme	arabinan	512:519	arg1	region					521:526	an arabinan region	509:526	an arabinan region composed of 1,5-linked Araf residues	509:563	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	5	31	theme	NO	727:728	arg1	release					730:736	NO release	727:736	NO release	727:736	PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL.					
32335112	3	32	theme	typical	472:478	arg1	xyloglucan					480:489	a typical xyloglucan	470:489	a typical xyloglucan	470:489	According to the IR, NMR spectra, monosaccharide composition, and methylation analyses, PSNP-1 was mainly composed of glucose and xylose residues, which form a typical xyloglucan.					
32335112	0	33	theme	polysaccharides	62:76	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunostimulatory activity of polysaccharides from Pyrus sinkiangensis Yu.					
32335112	0	33	theme	polysaccharides	62:76	arg1	activity					50:57	immunostimulatory activity	32:57	immunostimulatory activity	32:57	Structural characterization and immunostimulatory activity of polysaccharides from Pyrus sinkiangensis Yu.					
32335112	5	34	dep	viability	716:724	arg1	TNF-α					772:776	TNF-α	772:776	TNF-α	772:776	PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL.					
32335112	5	34	dep	viability	716:724	arg1	IL-6					763:766	IL-6	763:766	IL-6	763:766	PSNP-1 and PSNP-2 could stimulate the cell viability, NO release, and cytokine secretion (IL-6 and TNF-α) of RAW264.7 macrophages at the adosage of 250 μg/mL.					
32335112	1	35	theme	Yu	224:225	arg1	pulp					196:199	the pulp	192:199	the pulp of Pyrus sinkiangensis Yu	192:225	Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa) were isolated from the pulp of Pyrus sinkiangensis Yu.					
32335112	4	36	link	1,2-linked	618:627	arg1	Xylp					629:632	1,2-linked Xylp	618:632	1,2-linked Xylp	618:632	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	2	37	theme	combined	241:248	arg1	techniques					250:259	the combined techniques	237:259	the combined techniques of ion-exchange and gel permeation chromatography	237:309	by using the combined techniques of ion-exchange and gel permeation chromatography.					
32335112	1	38	dep	polysaccharides	119:133	arg1	104.7 kDa					144:152	104.7 kDa	144:152	104.7 kDa	144:152	Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa) were isolated from the pulp of Pyrus sinkiangensis Yu.					
32335112	1	38	dep	polysaccharides	119:133	arg1	PSNP-1					136:141	PSNP-1	136:141	PSNP-1	136:141	Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa) were isolated from the pulp of Pyrus sinkiangensis Yu.					
32335112	1	38	dep	polysaccharides	119:133	arg1	24.5 kDa					163:170	24.5 kDa	163:170	24.5 kDa	163:170	Two neutral polysaccharides (PSNP-1, 104.7 kDa; PSNP-2, 24.5 kDa) were isolated from the pulp of Pyrus sinkiangensis Yu.					
32335112	3	39	theme	NMR	333:335	arg1	spectra					337:343	NMR spectra	333:343	NMR spectra	333:343	According to the IR, NMR spectra, monosaccharide composition, and methylation analyses, PSNP-1 was mainly composed of glucose and xylose residues, which form a typical xyloglucan.					
32335112	4	40	dep	1,4-	639:642	arg1	1,4,6-linked					645:656	1,4,6-linked	645:656	1,4,6-linked	645:656	PSNP-2 contained an arabinan region composed of 1,5-linked Araf residues, a xyloglucan region that was mainly composed of t-, 1,2-linked Xylp, and 1,4-, 1,4,6-linked Glcp residues.					
32335112	6	41	theme	macrophage-mediated	885:903	arg1	activity					923:930	macrophage-mediated immunostimulatory activity	885:930	macrophage-mediated immunostimulatory activity	885:930	It was suggested that PSNP-1 and PSNP-2 may increase macrophage-mediated immunostimulatory activity.					
33413029	8	0	theme	favored	1129:1135	arg1	region					1137:1142	the favored region	1125:1142	the favored region	1125:1142	Using RAMPAGE the Ramachandran plot was generated, which predicted the accuracy of the 3D model with 91.5% amino acid residues in the favored region.					
33413029	11	1	theme	cellobiose	1351:1360	arg1	bonding					1362:1368	cellobiose bonding	1351:1368	cellobiose bonding	1351:1368	The key amino acid residues involved in cellobiose bonding are Val88, Asp106, Asp287, Tyr255, Arg170, Glu514.					
33413029	3	2	dep	components	308:317	arg1	exoglucanase					355:366	exoglucanase	355:366	exoglucanase	355:366	There are three components of cellulases: a) endoglucanase, b) exoglucanase and c) β-glucosidase which act synergistically in cellulose bioconversion.					
33413029	3	2	dep	components	308:317	arg1	β-glucosidase					375:387	β-glucosidase	375:387	β-glucosidase	375:387	There are three components of cellulases: a) endoglucanase, b) exoglucanase and c) β-glucosidase which act synergistically in cellulose bioconversion.					
33413029	3	2	dep	components	308:317	arg1	endoglucanase					337:349	endoglucanase	337:349	endoglucanase	337:349	There are three components of cellulases: a) endoglucanase, b) exoglucanase and c) β-glucosidase which act synergistically in cellulose bioconversion.					
33413029	6	3	theme	9.23	843:846	arg1	software					848:855	Modeller 9.23 software	834:855	Modeller 9.23 software	834:855	The protein structure of MtBgl3c is unknown, therefore an attempt has been made to model 3D structure using Modeller 9.23 software.					
33413029	4	4	with	enzymes	491:497	arg1	potential					512:520	a great potential	504:520	a great potential in bioethanol production	504:545	The cellulases are the third largest industrial enzymes with a great potential in bioethanol production.					
33413029	4	5	from	potential	512:520	arg1	production					536:545	bioethanol production	525:545	bioethanol production	525:545	The cellulases are the third largest industrial enzymes with a great potential in bioethanol production.					
33413029	9	6	theme	N-glycosylation	1165:1179	arg1	sites					1181:1185	The ion binding and N-glycosylation sites	1145:1185	sites	1181:1185	The ion binding and N-glycosylation sites were also predicted.					
33413029	6	7	theme	3D	815:816	arg1	structure					818:826	model 3D structure	809:826	model 3D structure	809:826	The protein structure of MtBgl3c is unknown, therefore an attempt has been made to model 3D structure using Modeller 9.23 software.					
33413029	7	8	theme	protein	870:876	arg1	model					878:882	The MtBgl3c protein model	858:882	The MtBgl3c protein model generated	858:892	The MtBgl3c protein model generated was validated from Verify 3D and ERRAT scores of 89.37% and 71.25%, respectively derived from SAVES.					
33413029	1	9	theme	industrial	170:179	arg1	applications					181:192	industrial applications	170:192	industrial applications	170:192	Cellulases are the enzymes with diverse range of industrial applications.					
33413029	11	10	theme	amino	1319:1323	arg1	residues					1330:1337	The key amino acid residues	1311:1337	The key amino acid residues involved in cellobiose bonding	1311:1368	The key amino acid residues involved in cellobiose bonding are Val88, Asp106, Asp287, Tyr255, Arg170, Glu514.					
33413029	11	10	theme	amino	1319:1323	arg1	Asp106					1381:1386	Asp106	1381:1386	Asp106	1381:1386	The key amino acid residues involved in cellobiose bonding are Val88, Asp106, Asp287, Tyr255, Arg170, Glu514.					
33413029	8	11	theme	Ramachandran	1013:1024	arg1	plot					1026:1029	the Ramachandran plot	1009:1029	the Ramachandran plot	1009:1029	Using RAMPAGE the Ramachandran plot was generated, which predicted the accuracy of the 3D model with 91.5% amino acid residues in the favored region.					
33413029	15	12	theme	experimental	1839:1850	arg1	data					1852:1855	experimental data	1839:1855	experimental data	1839:1855	The present study provides insight into structural characteristics of the MtBgl3c which can be further validated by experimental data.					
33413029	13	13	theme	cellobiose	1506:1515	arg1	lower					1538:1542	lower	1538:1542	lower	1538:1542	The dock score of cellobiose with MtBgl3c is much lower (-6.46 kcal/mol) than that of glucose (-5.61 kcal/mol), suggesting its high affinity for cellobiose.					
33413029	13	13	theme	cellobiose	1506:1515	arg1	score					1497:1501	The dock score	1488:1501	The dock score of cellobiose with MtBgl3c	1488:1528	The dock score of cellobiose with MtBgl3c is much lower (-6.46 kcal/mol) than that of glucose (-5.61 kcal/mol), suggesting its high affinity for cellobiose.					
33413029	16	14	theme	Highlights3D	1858:1869	arg1	structure					1871:1879	Highlights3D structure	1858:1879	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila	1858:1936	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	4	15	theme	largest	472:478	arg1	cellulases					447:456	The cellulases	443:456	The cellulases	443:456	The cellulases are the third largest industrial enzymes with a great potential in bioethanol production.					
33413029	4	15	theme	largest	472:478	arg1	enzymes					491:497	the third largest industrial enzymes	462:497	the third largest industrial enzymes	462:497	The cellulases are the third largest industrial enzymes with a great potential in bioethanol production.					
33413029	2	16	theme	monomeric	229:237	arg1	units					247:251	monomeric glucose units	229:251	monomeric glucose units	229:251	Cellulases degrade cellulose into monomeric glucose units by hydrolysing β-1,4-glycosidic bonds.					
33413029	7	17	theme	%	948:948	arg1	3D					920:921	3D	920:921	3D	920:921	The MtBgl3c protein model generated was validated from Verify 3D and ERRAT scores of 89.37% and 71.25%, respectively derived from SAVES.					
33413029	7	17	theme	%	948:948	arg1	scores					933:938	ERRAT scores	927:938	ERRAT scores	927:938	The MtBgl3c protein model generated was validated from Verify 3D and ERRAT scores of 89.37% and 71.25%, respectively derived from SAVES.					
33413029	16	18	theme	glucose	2267:2273	arg1	data					2288:2291	its glucose toleranceThe data	2263:2291	its glucose toleranceThe data	2263:2291	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	13	19	with	score	1497:1501	arg1	MtBgl3c					1522:1528	MtBgl3c	1522:1528	MtBgl3c	1522:1528	The dock score of cellobiose with MtBgl3c is much lower (-6.46 kcal/mol) than that of glucose (-5.61 kcal/mol), suggesting its high affinity for cellobiose.					
33413029	2	20	theme	hydrolysing	256:266	arg1	bonds					285:289	hydrolysing β-1,4-glycosidic bonds	256:289	hydrolysing β-1,4-glycosidic bonds	256:289	Cellulases degrade cellulose into monomeric glucose units by hydrolysing β-1,4-glycosidic bonds.					
33413029	5	21	theme	thermophila	627:637	arg1	β-glucosidase					573:585	a β-glucosidase	571:585	a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c)	571:647	In this investigation, a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c) was analysed for its structural characterization using in silico approaches.					
33413029	5	22	theme	in	704:705	arg1	approaches					714:723	in silico approaches	704:723	in silico approaches	704:723	In this investigation, a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c) was analysed for its structural characterization using in silico approaches.					
33413029	4	23	theme	bioethanol	525:534	arg1	production					536:545	bioethanol production	525:545	bioethanol production	525:545	The cellulases are the third largest industrial enzymes with a great potential in bioethanol production.					
33413029	16	24	theme	higher	2346:2351	arg1	efficiencyCommunicated					2363:2384	higher catalytic efficiencyCommunicated	2346:2384	higher catalytic efficiencyCommunicated	2346:2384	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	7	25	theme	%	959:959	arg1	3D					920:921	3D	920:921	3D	920:921	The MtBgl3c protein model generated was validated from Verify 3D and ERRAT scores of 89.37% and 71.25%, respectively derived from SAVES.					
33413029	7	25	theme	%	959:959	arg1	scores					933:938	ERRAT scores	927:938	ERRAT scores	927:938	The MtBgl3c protein model generated was validated from Verify 3D and ERRAT scores of 89.37% and 71.25%, respectively derived from SAVES.					
33413029	13	26	theme	high	1615:1618	arg1	affinity					1620:1627	its high affinity	1611:1627	its high affinity for cellobiose	1611:1642	The dock score of cellobiose with MtBgl3c is much lower (-6.46 kcal/mol) than that of glucose (-5.61 kcal/mol), suggesting its high affinity for cellobiose.					
33413029	16	27	theme	confirmedDocking	2203:2218	arg1	analysis					2220:2227	confirmedDocking analysis	2203:2227	confirmedDocking analysis of MtBgl3c with glucose	2203:2251	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	0	28	theme	molecular	102:110	arg1	docking					112:118	molecular docking	102:118	molecular docking	102:118	Structural aspects of β-glucosidase of Myceliophthora thermophila (MtBgl3c) by homology modelling and molecular docking.					
33413029	3	29	theme	cellulose	418:426	arg1	bioconversion					428:440	cellulose bioconversion	418:440	cellulose bioconversion	418:440	There are three components of cellulases: a) endoglucanase, b) exoglucanase and c) β-glucosidase which act synergistically in cellulose bioconversion.					
33413029	15	30	theme	structural	1763:1772	arg1	characteristics					1774:1788	structural characteristics	1763:1788	structural characteristics of the MtBgl3c which can be further validated by experimental data	1763:1855	The present study provides insight into structural characteristics of the MtBgl3c which can be further validated by experimental data.					
33413029	6	31	theme	protein	730:736	arg1	structure					738:746	The protein structure	726:746	The protein structure of MtBgl3c	726:757	The protein structure of MtBgl3c is unknown, therefore an attempt has been made to model 3D structure using Modeller 9.23 software.					
33413029	6	31	theme	protein	730:736	arg1	unknown					762:768	unknown	762:768	unknown	762:768	The protein structure of MtBgl3c is unknown, therefore an attempt has been made to model 3D structure using Modeller 9.23 software.					
33413029	0	32	theme	Structural	0:9	arg1	aspects					11:17	Structural aspects	0:17	Structural aspects of β-glucosidase of Myceliophthora thermophila (MtBgl3c) by homology modelling and molecular docking.	0:119	Structural aspects of β-glucosidase of Myceliophthora thermophila (MtBgl3c) by homology modelling and molecular docking.					
33413029	5	33	theme	fungus	605:610	arg1	MtBgl3c					640:646	MtBgl3c	640:646	MtBgl3c	640:646	In this investigation, a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c) was analysed for its structural characterization using in silico approaches.					
33413029	5	33	theme	fungus	605:610	arg1	thermophila					627:637	a thermophilic fungus Myceliophthora thermophila	590:637	a thermophilic fungus Myceliophthora thermophila (MtBgl3c)	590:647	In this investigation, a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c) was analysed for its structural characterization using in silico approaches.					
33413029	2	34	theme	β-1,4-glycosidic	268:283	arg1	bonds					285:289	hydrolysing β-1,4-glycosidic bonds	256:289	hydrolysing β-1,4-glycosidic bonds	256:289	Cellulases degrade cellulose into monomeric glucose units by hydrolysing β-1,4-glycosidic bonds.					
33413029	15	35	theme	present	1727:1733	arg1	study					1735:1739	The present study	1723:1739	The present study	1723:1739	The present study provides insight into structural characteristics of the MtBgl3c which can be further validated by experimental data.					
33413029	12	36	theme	MtBgl3c	1463:1469	arg1	MtBgl3c					1463:1469	MtBgl3c	1463:1469	MtBgl3c	1463:1469	The catalytic conserved amino residues of MtBgl3c were identified.					
33413029	12	36	theme	MtBgl3c	1463:1469	arg1	residues					1451:1458	The catalytic conserved amino residues	1421:1458	The catalytic conserved amino residues of MtBgl3c	1421:1469	The catalytic conserved amino residues of MtBgl3c were identified.					
33413029	16	37	theme	analysesThe	1979:1989	arg1	Glu514					2046:2051	Glu514	2046:2051	Glu514	2046:2051	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	37	theme	analysesThe	1979:1989	arg1	Arg170					2035:2040	Arg170	2035:2040	Arg170	2035:2040	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	37	theme	analysesThe	1979:1989	arg1	residues					2002:2009	computational analysesThe amino acid residues	1965:2009	computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514	1965:2051	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	37	theme	analysesThe	1979:1989	arg1	Asp287					2019:2024	Asp287	2019:2024	Asp287	2019:2024	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	7	38	attach	derived	975:981	arg1	SAVES					988:992	SAVES	988:992	SAVES	988:992	The MtBgl3c protein model generated was validated from Verify 3D and ERRAT scores of 89.37% and 71.25%, respectively derived from SAVES.					
33413029	7	38	attach	derived	975:981	arg2	%					948:948	89.37%	943:948	89.37%	943:948	The MtBgl3c protein model generated was validated from Verify 3D and ERRAT scores of 89.37% and 71.25%, respectively derived from SAVES.					
33413029	10	39	theme	binding	1285:1291	arg1	sites					1293:1297	the most favorable binding sites	1266:1297	the most favorable binding sites of MtBgl3c	1266:1308	The generated model was docked with cellobiose to predict the most favorable binding sites of MtBgl3c.					
33413029	10	39	theme	binding	1285:1291	arg1	MtBgl3c					1302:1308	MtBgl3c	1302:1308	MtBgl3c	1302:1308	The generated model was docked with cellobiose to predict the most favorable binding sites of MtBgl3c.					
33413029	8	40	theme	model	1085:1089	arg1	accuracy					1066:1073	the accuracy	1062:1073	the accuracy of the 3D model	1062:1089	Using RAMPAGE the Ramachandran plot was generated, which predicted the accuracy of the 3D model with 91.5% amino acid residues in the favored region.					
33413029	8	41	theme	91.5	1096:1099	arg1	%					1100:1100	%	1100:1100	%	1100:1100	Using RAMPAGE the Ramachandran plot was generated, which predicted the accuracy of the 3D model with 91.5% amino acid residues in the favored region.					
33413029	12	42	theme	conserved	1435:1443	arg1	MtBgl3c					1463:1469	MtBgl3c	1463:1469	MtBgl3c	1463:1469	The catalytic conserved amino residues of MtBgl3c were identified.					
33413029	12	42	theme	conserved	1435:1443	arg1	residues					1451:1458	The catalytic conserved amino residues	1421:1458	The catalytic conserved amino residues of MtBgl3c	1421:1469	The catalytic conserved amino residues of MtBgl3c were identified.					
33413029	8	43	theme	amino	1102:1106	arg1	residues					1113:1120	91.5% amino acid residues	1096:1120	91.5% amino acid residues in the favored region	1096:1142	Using RAMPAGE the Ramachandran plot was generated, which predicted the accuracy of the 3D model with 91.5% amino acid residues in the favored region.					
33413029	16	44	with	interaction	2143:2153	arg1	cellobiose					2171:2180	cellobiose	2171:2180	cellobiose	2171:2180	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	45	theme	MtBgl3c	2158:2164	arg1	bindingDocking					2124:2137	substrate bindingDocking	2114:2137	substrate bindingDocking	2114:2137	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	45	theme	MtBgl3c	2158:2164	arg1	interaction					2143:2153	interaction	2143:2153	interaction of MtBgl3c with cellobiose	2143:2180	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	7	46	theme	ERRAT	927:931	arg1	scores					933:938	ERRAT scores	927:938	ERRAT scores	927:938	The MtBgl3c protein model generated was validated from Verify 3D and ERRAT scores of 89.37% and 71.25%, respectively derived from SAVES.					
33413029	16	47	theme	Myceliophthora	1911:1924	arg1	MtBgl3c					1899:1905	MtBgl3c	1899:1905	MtBgl3c	1899:1905	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	47	theme	Myceliophthora	1911:1924	arg1	β-glucosidase					1884:1896	β-glucosidase	1884:1896	β-glucosidase (MtBgl3c) of Myceliophthora thermophila	1884:1936	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	48	with	bindingDocking	2124:2137	arg1	cellobiose					2171:2180	cellobiose	2171:2180	cellobiose	2171:2180	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	49	theme	important	2096:2104	arg1	role					2106:2109	catalytically important role	2082:2109	catalytically important role	2082:2109	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	15	50	theme	MtBgl3c	1797:1803	arg1	characteristics					1774:1788	structural characteristics	1763:1788	structural characteristics of the MtBgl3c which can be further validated by experimental data	1763:1855	The present study provides insight into structural characteristics of the MtBgl3c which can be further validated by experimental data.					
33413029	5	51	theme	structural	670:679	arg1	characterization					681:696	its structural characterization	666:696	its structural characterization using in silico approaches	666:723	In this investigation, a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c) was analysed for its structural characterization using in silico approaches.					
33413029	9	52	theme	ion	1149:1151	arg1	binding					1153:1159	The ion binding and N-glycosylation sites	1145:1185	binding	1153:1159	The ion binding and N-glycosylation sites were also predicted.					
33413029	16	53	theme	catalytic	2353:2361	arg1	efficiencyCommunicated					2363:2384	higher catalytic efficiencyCommunicated	2346:2384	higher catalytic efficiencyCommunicated	2346:2384	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	6	54	theme	Modeller	834:841	arg1	software					848:855	Modeller 9.23 software	834:855	Modeller 9.23 software	834:855	The protein structure of MtBgl3c is unknown, therefore an attempt has been made to model 3D structure using Modeller 9.23 software.					
33413029	14	55	theme	MtBgl3c	1665:1671	arg1	data					1657:1660	The docking data	1645:1660	The docking data of MtBgl3c with glucose	1645:1684	The docking data of MtBgl3c with glucose illustrate its tolerance to glucose.					
33413029	8	56	dep	generated	1035:1043	arg1	Using					995:999	Using	995:999	Using RAMPAGE	995:1007	Using RAMPAGE the Ramachandran plot was generated, which predicted the accuracy of the 3D model with 91.5% amino acid residues in the favored region.					
33413029	16	57	dep	suggested	2253:2261	arg1	useful					2302:2307	useful	2302:2307	useful	2302:2307	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	11	58	theme	key	1315:1317	arg1	residues					1330:1337	The key amino acid residues	1311:1337	The key amino acid residues involved in cellobiose bonding	1311:1368	The key amino acid residues involved in cellobiose bonding are Val88, Asp106, Asp287, Tyr255, Arg170, Glu514.					
33413029	11	58	theme	key	1315:1317	arg1	Asp106					1381:1386	Asp106	1381:1386	Asp106	1381:1386	The key amino acid residues involved in cellobiose bonding are Val88, Asp106, Asp287, Tyr255, Arg170, Glu514.					
33413029	6	59	theme	model	809:813	arg1	structure					818:826	model 3D structure	809:826	model 3D structure	809:826	The protein structure of MtBgl3c is unknown, therefore an attempt has been made to model 3D structure using Modeller 9.23 software.					
33413029	1	60	theme	applications	181:192	arg1	range					161:165	diverse range	153:165	diverse range of industrial applications	153:192	Cellulases are the enzymes with diverse range of industrial applications.					
33413029	7	61	dep	3D	920:921	arg1	Verify					913:918	Verify	913:918	Verify	913:918	The MtBgl3c protein model generated was validated from Verify 3D and ERRAT scores of 89.37% and 71.25%, respectively derived from SAVES.					
33413029	7	62	theme	MtBgl3c	862:868	arg1	model					878:882	The MtBgl3c protein model	858:882	The MtBgl3c protein model generated	858:892	The MtBgl3c protein model generated was validated from Verify 3D and ERRAT scores of 89.37% and 71.25%, respectively derived from SAVES.					
33413029	9	63	gly	N-glycosylation	1165:1179	arg2	sites					1181:1185	The ion binding and N-glycosylation sites	1145:1185	sites	1181:1185	The ion binding and N-glycosylation sites were also predicted.					
33413029	4	64	theme	third	466:470	arg1	cellulases					447:456	The cellulases	443:456	The cellulases	443:456	The cellulases are the third largest industrial enzymes with a great potential in bioethanol production.					
33413029	4	64	theme	third	466:470	arg1	enzymes					491:497	the third largest industrial enzymes	462:497	the third largest industrial enzymes	462:497	The cellulases are the third largest industrial enzymes with a great potential in bioethanol production.					
33413029	14	65	theme	docking	1649:1655	arg1	data					1657:1660	The docking data	1645:1660	The docking data of MtBgl3c with glucose	1645:1684	The docking data of MtBgl3c with glucose illustrate its tolerance to glucose.					
33413029	4	66	theme	industrial	480:489	arg1	cellulases					447:456	The cellulases	443:456	The cellulases	443:456	The cellulases are the third largest industrial enzymes with a great potential in bioethanol production.					
33413029	4	66	theme	industrial	480:489	arg1	enzymes					491:497	the third largest industrial enzymes	462:497	the third largest industrial enzymes	462:497	The cellulases are the third largest industrial enzymes with a great potential in bioethanol production.					
33413029	16	67	dep	Myceliophthora	1911:1924	arg1	thermophila					1926:1936	Myceliophthora thermophila	1911:1936	Myceliophthora thermophila	1911:1936	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	11	68	theme	acid	1325:1328	arg1	residues					1330:1337	The key amino acid residues	1311:1337	The key amino acid residues involved in cellobiose bonding	1311:1368	The key amino acid residues involved in cellobiose bonding are Val88, Asp106, Asp287, Tyr255, Arg170, Glu514.					
33413029	11	68	theme	acid	1325:1328	arg1	Asp106					1381:1386	Asp106	1381:1386	Asp106	1381:1386	The key amino acid residues involved in cellobiose bonding are Val88, Asp106, Asp287, Tyr255, Arg170, Glu514.					
33413029	2	69	theme	glucose	239:245	arg1	units					247:251	monomeric glucose units	229:251	monomeric glucose units	229:251	Cellulases degrade cellulose into monomeric glucose units by hydrolysing β-1,4-glycosidic bonds.					
33413029	16	70	theme	toleranceThe	2275:2286	arg1	data					2288:2291	its glucose toleranceThe data	2263:2291	its glucose toleranceThe data	2263:2291	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	71	dep	residues	2002:2009	arg1	Glu514					2046:2051	Glu514	2046:2051	Glu514	2046:2051	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	71	dep	residues	2002:2009	arg1	Arg170					2035:2040	Arg170	2035:2040	Arg170	2035:2040	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	71	dep	residues	2002:2009	arg1	residues					2002:2009	computational analysesThe amino acid residues	1965:2009	computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514	1965:2051	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	71	dep	residues	2002:2009	arg1	Asp287					2019:2024	Asp287	2019:2024	Asp287	2019:2024	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	3	72	theme	cellulases	322:331	arg1	components					308:317	three components	302:317	three components of cellulases: a) endoglucanase, b) exoglucanase and c) β-glucosidase which act synergistically in cellulose bioconversion	302:440	There are three components of cellulases: a) endoglucanase, b) exoglucanase and c) β-glucosidase which act synergistically in cellulose bioconversion.					
33413029	10	73	theme	generated	1212:1220	arg1	model					1222:1226	The generated model	1208:1226	The generated model	1208:1226	The generated model was docked with cellobiose to predict the most favorable binding sites of MtBgl3c.					
33413029	13	74	theme	dock	1492:1495	arg1	lower					1538:1542	lower	1538:1542	lower	1538:1542	The dock score of cellobiose with MtBgl3c is much lower (-6.46 kcal/mol) than that of glucose (-5.61 kcal/mol), suggesting its high affinity for cellobiose.					
33413029	13	74	theme	dock	1492:1495	arg1	score					1497:1501	The dock score	1488:1501	The dock score of cellobiose with MtBgl3c	1488:1528	The dock score of cellobiose with MtBgl3c is much lower (-6.46 kcal/mol) than that of glucose (-5.61 kcal/mol), suggesting its high affinity for cellobiose.					
33413029	0	75	theme	homology	79:86	arg1	modelling					88:96	homology modelling	79:96	homology modelling	79:96	Structural aspects of β-glucosidase of Myceliophthora thermophila (MtBgl3c) by homology modelling and molecular docking.					
33413029	16	76	with	MtBgl3c	2232:2238	arg1	glucose					2245:2251	glucose	2245:2251	glucose	2245:2251	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	1	77	theme	diverse	153:159	arg1	range					161:165	diverse range	153:165	diverse range of industrial applications	153:192	Cellulases are the enzymes with diverse range of industrial applications.					
33413029	1	78	with	enzymes	140:146	arg1	range					161:165	diverse range	153:165	diverse range of industrial applications	153:192	Cellulases are the enzymes with diverse range of industrial applications.					
33413029	6	79	theme	MtBgl3c	751:757	arg1	structure					738:746	The protein structure	726:746	The protein structure of MtBgl3c	726:757	The protein structure of MtBgl3c is unknown, therefore an attempt has been made to model 3D structure using Modeller 9.23 software.					
33413029	6	79	theme	MtBgl3c	751:757	arg1	unknown					762:768	unknown	762:768	unknown	762:768	The protein structure of MtBgl3c is unknown, therefore an attempt has been made to model 3D structure using Modeller 9.23 software.					
33413029	10	80	theme	favorable	1275:1283	arg1	sites					1293:1297	the most favorable binding sites	1266:1297	the most favorable binding sites of MtBgl3c	1266:1308	The generated model was docked with cellobiose to predict the most favorable binding sites of MtBgl3c.					
33413029	10	80	theme	favorable	1275:1283	arg1	MtBgl3c					1302:1308	MtBgl3c	1302:1308	MtBgl3c	1302:1308	The generated model was docked with cellobiose to predict the most favorable binding sites of MtBgl3c.					
33413029	14	81	with	data	1657:1660	arg1	glucose					1678:1684	glucose	1678:1684	glucose	1678:1684	The docking data of MtBgl3c with glucose illustrate its tolerance to glucose.					
33413029	4	82	theme	great	506:510	arg1	potential					512:520	a great potential	504:520	a great potential in bioethanol production	504:545	The cellulases are the third largest industrial enzymes with a great potential in bioethanol production.					
33413029	16	83	theme	MtBgl3c	2232:2238	arg1	analysis					2220:2227	confirmedDocking analysis	2203:2227	confirmedDocking analysis of MtBgl3c with glucose	2203:2251	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	5	84	theme	thermophilic	592:603	arg1	MtBgl3c					640:646	MtBgl3c	640:646	MtBgl3c	640:646	In this investigation, a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c) was analysed for its structural characterization using in silico approaches.					
33413029	5	84	theme	thermophilic	592:603	arg1	thermophila					627:637	a thermophilic fungus Myceliophthora thermophila	590:637	a thermophilic fungus Myceliophthora thermophila (MtBgl3c)	590:647	In this investigation, a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c) was analysed for its structural characterization using in silico approaches.					
33413029	16	85	theme	computational	1965:1977	arg1	Glu514					2046:2051	Glu514	2046:2051	Glu514	2046:2051	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	85	theme	computational	1965:1977	arg1	Arg170					2035:2040	Arg170	2035:2040	Arg170	2035:2040	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	85	theme	computational	1965:1977	arg1	residues					2002:2009	computational analysesThe amino acid residues	1965:2009	computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514	1965:2051	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	85	theme	computational	1965:1977	arg1	Asp287					2019:2024	Asp287	2019:2024	Asp287	2019:2024	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	5	86	dep	in	704:705	arg1	silico					707:712	silico	707:712	silico	707:712	In this investigation, a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c) was analysed for its structural characterization using in silico approaches.					
33413029	10	87	theme	MtBgl3c	1302:1308	arg1	sites					1293:1297	the most favorable binding sites	1266:1297	the most favorable binding sites of MtBgl3c	1266:1308	The generated model was docked with cellobiose to predict the most favorable binding sites of MtBgl3c.					
33413029	10	87	theme	MtBgl3c	1302:1308	arg1	MtBgl3c					1302:1308	MtBgl3c	1302:1308	MtBgl3c	1302:1308	The generated model was docked with cellobiose to predict the most favorable binding sites of MtBgl3c.					
33413029	0	88	theme	β-glucosidase	22:34	arg1	aspects					11:17	Structural aspects	0:17	Structural aspects of β-glucosidase of Myceliophthora thermophila (MtBgl3c) by homology modelling and molecular docking.	0:119	Structural aspects of β-glucosidase of Myceliophthora thermophila (MtBgl3c) by homology modelling and molecular docking.					
33413029	8	89	theme	3D	1082:1083	arg1	model					1085:1089	the 3D model	1078:1089	the 3D model	1078:1089	Using RAMPAGE the Ramachandran plot was generated, which predicted the accuracy of the 3D model with 91.5% amino acid residues in the favored region.					
33413029	5	90	theme	Myceliophthora	612:625	arg1	MtBgl3c					640:646	MtBgl3c	640:646	MtBgl3c	640:646	In this investigation, a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c) was analysed for its structural characterization using in silico approaches.					
33413029	5	90	theme	Myceliophthora	612:625	arg1	thermophila					627:637	a thermophilic fungus Myceliophthora thermophila	590:637	a thermophilic fungus Myceliophthora thermophila (MtBgl3c)	590:647	In this investigation, a β-glucosidase of a thermophilic fungus Myceliophthora thermophila (MtBgl3c) was analysed for its structural characterization using in silico approaches.					
33413029	16	91	theme	amino	1991:1995	arg1	Glu514					2046:2051	Glu514	2046:2051	Glu514	2046:2051	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	91	theme	amino	1991:1995	arg1	Arg170					2035:2040	Arg170	2035:2040	Arg170	2035:2040	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	91	theme	amino	1991:1995	arg1	residues					2002:2009	computational analysesThe amino acid residues	1965:2009	computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514	1965:2051	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	91	theme	amino	1991:1995	arg1	Asp287					2019:2024	Asp287	2019:2024	Asp287	2019:2024	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	92	theme	acid	1997:2000	arg1	Glu514					2046:2051	Glu514	2046:2051	Glu514	2046:2051	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	92	theme	acid	1997:2000	arg1	Arg170					2035:2040	Arg170	2035:2040	Arg170	2035:2040	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	92	theme	acid	1997:2000	arg1	residues					2002:2009	computational analysesThe amino acid residues	1965:2009	computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514	1965:2051	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	92	theme	acid	1997:2000	arg1	Asp287					2019:2024	Asp287	2019:2024	Asp287	2019:2024	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	0	93	theme	Myceliophthora	39:52	arg1	β-glucosidase					22:34	β-glucosidase	22:34	β-glucosidase of Myceliophthora thermophila (MtBgl3c)	22:74	Structural aspects of β-glucosidase of Myceliophthora thermophila (MtBgl3c) by homology modelling and molecular docking.					
33413029	16	94	theme	β-glucosidase	1884:1896	arg1	structure					1871:1879	Highlights3D structure	1858:1879	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila	1858:1936	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	16	95	theme	substrate	2114:2122	arg1	bindingDocking					2124:2137	substrate bindingDocking	2114:2137	substrate bindingDocking	2114:2137	Highlights3D structure of β-glucosidase (MtBgl3c) of Myceliophthora thermophila is being proposed based on computational analysesThe amino acid residues Asp106, Asp287, Tyr255, Arg170 and Glu514 have been identified to play catalytically important role in substrate bindingDocking and interaction of MtBgl3c with cellobiose and glucose has been confirmedDocking analysis of MtBgl3c with glucose suggested its glucose toleranceThe data would be useful in engineering enzymes for attaining higher catalytic efficiencyCommunicated by Ramaswamy H. Sarma.					
33413029	8	96	theme	%	1100:1100	arg1	residues					1113:1120	91.5% amino acid residues	1096:1120	91.5% amino acid residues in the favored region	1096:1142	Using RAMPAGE the Ramachandran plot was generated, which predicted the accuracy of the 3D model with 91.5% amino acid residues in the favored region.					
33413029	8	97	from	residues	1113:1120	arg1	region					1137:1142	the favored region	1125:1142	the favored region	1125:1142	Using RAMPAGE the Ramachandran plot was generated, which predicted the accuracy of the 3D model with 91.5% amino acid residues in the favored region.					
33413029	0	98	dep	Myceliophthora	39:52	arg1	thermophila					54:64	Myceliophthora thermophila	39:64	Myceliophthora thermophila (MtBgl3c)	39:74	Structural aspects of β-glucosidase of Myceliophthora thermophila (MtBgl3c) by homology modelling and molecular docking.					
33413029	8	99	theme	acid	1108:1111	arg1	residues					1113:1120	91.5% amino acid residues	1096:1120	91.5% amino acid residues in the favored region	1096:1142	Using RAMPAGE the Ramachandran plot was generated, which predicted the accuracy of the 3D model with 91.5% amino acid residues in the favored region.					
33413029	12	100	theme	catalytic	1425:1433	arg1	MtBgl3c					1463:1469	MtBgl3c	1463:1469	MtBgl3c	1463:1469	The catalytic conserved amino residues of MtBgl3c were identified.					
33413029	12	100	theme	catalytic	1425:1433	arg1	residues					1451:1458	The catalytic conserved amino residues	1421:1458	The catalytic conserved amino residues of MtBgl3c	1421:1469	The catalytic conserved amino residues of MtBgl3c were identified.					
33413029	12	101	theme	amino	1445:1449	arg1	MtBgl3c					1463:1469	MtBgl3c	1463:1469	MtBgl3c	1463:1469	The catalytic conserved amino residues of MtBgl3c were identified.					
33413029	12	101	theme	amino	1445:1449	arg1	residues					1451:1458	The catalytic conserved amino residues	1421:1458	The catalytic conserved amino residues of MtBgl3c	1421:1469	The catalytic conserved amino residues of MtBgl3c were identified.					
33413029	13	102	dep	lower	1538:1542	arg1	-6.46 kcal/mol					1545:1558	-6.46 kcal/mol	1545:1558	-6.46 kcal/mol	1545:1558	The dock score of cellobiose with MtBgl3c is much lower (-6.46 kcal/mol) than that of glucose (-5.61 kcal/mol), suggesting its high affinity for cellobiose.					
35227466	0	0	theme	Sagittaria	89:98	arg1	polysaccharides					116:130	Sagittaria sagittifolia L. polysaccharides	89:130	Sagittaria sagittifolia L. polysaccharides	89:130	Characterization, antioxidant, antineoplastic and immune activities of selenium modified Sagittaria sagittifolia L. polysaccharides.					
35227466	3	1	theme	force	703:707	arg1	AFM					721:723	AFM	721:723	AFM	721:723	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	3	1	theme	force	703:707	arg1	microscope					709:718	atomic force microscope	696:718	atomic force microscope (AFM)	696:724	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	9	2	theme	medicine	1577:1584	arg1	industry					1586:1593	medicine industry	1577:1593	medicine industry	1577:1593	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	4	3	contain	contained	773:781	arg1	Se-PSSP					765:771	Se-PSSP	765:771	Se-PSSP	765:771	The results showed that both PSSP and Se-PSSP contained a pyranoid polysaccharide linked by α-glycosidic bonds in the main chain.					
35227466	4	3	contain	contained	773:781	arg1	PSSP					756:759	PSSP	756:759	PSSP	756:759	The results showed that both PSSP and Se-PSSP contained a pyranoid polysaccharide linked by α-glycosidic bonds in the main chain.					
35227466	4	3	contain	contained	773:781	arg2	polysaccharide					794:807	a pyranoid polysaccharide	783:807	a pyranoid polysaccharide linked by α-glycosidic bonds in the main chain	783:854	The results showed that both PSSP and Se-PSSP contained a pyranoid polysaccharide linked by α-glycosidic bonds in the main chain.					
35227466	3	4	theme	monosaccharide	511:524	arg1	composition					526:536	the monosaccharide composition	507:536	the monosaccharide composition	507:536	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	6	5	with	xylose	1008:1013	arg1	percentages					1026:1036	molar percentages	1020:1036	molar percentages of 55.82%, 14.86% and 14.35%, respectively	1020:1079	PSSP (47.12 kDa) was mainly composed of glucose, mannose and xylose with molar percentages of 55.82%, 14.86% and 14.35%, respectively.					
35227466	9	6	theme	industry	1586:1593	arg1	field					1547:1551	the field	1543:1551	the field of functional foods and medicine industry	1543:1593	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	1	7	theme	supplement	171:180	arg1	selenium					190:197	supplement organic selenium	171:197	supplement organic selenium	171:197	This study proposed an optimal way to supplement organic selenium, boost polysaccharides solubility, antioxidant, anticancer, immune responses.					
35227466	5	8	theme	three-helix	921:931	arg1	conformation					933:944	three-helix conformation	921:944	three-helix conformation	921:944	In addition, PSSP and Se-PSSP were amorphous morphology without three-helix conformation.					
35227466	0	9	theme	L.	113:114	arg1	polysaccharides					116:130	Sagittaria sagittifolia L. polysaccharides	89:130	Sagittaria sagittifolia L. polysaccharides	89:130	Characterization, antioxidant, antineoplastic and immune activities of selenium modified Sagittaria sagittifolia L. polysaccharides.					
35227466	9	10	theme	functional	1556:1565	arg1	foods					1567:1571	functional foods	1556:1571	functional foods	1556:1571	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	6	11	with	glucose	987:993	arg1	percentages					1026:1036	molar percentages	1020:1036	molar percentages of 55.82%, 14.86% and 14.35%, respectively	1020:1079	PSSP (47.12 kDa) was mainly composed of glucose, mannose and xylose with molar percentages of 55.82%, 14.86% and 14.35%, respectively.					
35227466	2	12	theme	Sagittaria	315:324	arg1	L					339:339	Sagittaria sagittifolia L	315:339	Sagittaria sagittifolia L. (PSSP)	315:347	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	2	12	theme	Sagittaria	315:324	arg1	PSSP					343:346	PSSP	343:346	PSSP	343:346	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	7	13	with	glucose	1124:1130	arg1	percentages					1165:1175	molar percentages	1159:1175	molar percentages of 26.49%, 18.76% and 18.14%, respectively	1159:1218	Se-PSSP (16.82 kDa) is mainly composed of glucose, xylose and galactose with molar percentages of 26.49%, 18.76% and 18.14%, respectively.					
35227466	0	14	theme	sagittifolia	100:111	arg1	polysaccharides					116:130	Sagittaria sagittifolia L. polysaccharides	89:130	Sagittaria sagittifolia L. polysaccharides	89:130	Characterization, antioxidant, antineoplastic and immune activities of selenium modified Sagittaria sagittifolia L. polysaccharides.					
35227466	9	15	theme	immunomodulatory	1516:1531	arg1	agents					1533:1538	immunomodulatory agents	1516:1538	immunomodulatory agents	1516:1538	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	7	16	theme	molar	1159:1163	arg1	percentages					1165:1175	molar percentages	1159:1175	molar percentages of 26.49%, 18.76% and 18.14%, respectively	1159:1218	Se-PSSP (16.82 kDa) is mainly composed of glucose, xylose and galactose with molar percentages of 26.49%, 18.76% and 18.14%, respectively.					
35227466	7	17	theme	%	1185:1185	arg1	percentages					1165:1175	molar percentages	1159:1175	molar percentages of 26.49%, 18.76% and 18.14%, respectively	1159:1218	Se-PSSP (16.82 kDa) is mainly composed of glucose, xylose and galactose with molar percentages of 26.49%, 18.76% and 18.14%, respectively.					
35227466	3	18	theme	UV-visible	566:575	arg1	spectrometry					577:588	UV-visible spectrometry	566:588	UV-visible spectrometry	566:588	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	1	19	theme	organic	182:188	arg1	selenium					190:197	supplement organic selenium	171:197	supplement organic selenium	171:197	This study proposed an optimal way to supplement organic selenium, boost polysaccharides solubility, antioxidant, anticancer, immune responses.					
35227466	5	20	from	morphology	902:911	arg1	addition					860:867	addition	860:867	addition	860:867	In addition, PSSP and Se-PSSP were amorphous morphology without three-helix conformation.					
35227466	2	21	theme	polysaccharide	288:301	arg1	fraction					303:310	A purified polysaccharide fraction	277:310	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP)	277:347	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	6	22	theme	%	1054:1054	arg1	percentages					1026:1036	molar percentages	1020:1036	molar percentages of 55.82%, 14.86% and 14.35%, respectively	1020:1079	PSSP (47.12 kDa) was mainly composed of glucose, mannose and xylose with molar percentages of 55.82%, 14.86% and 14.35%, respectively.					
35227466	2	23	theme	immune	457:462	arg1	characteristics					408:422	its characteristics	404:422	its characteristics	404:422	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	2	23	theme	immune	457:462	arg1	activities					464:473	antioxidant, antineoplastic and immune activities	425:473	antioxidant, antineoplastic and immune activities	425:473	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	4	24	from	bonds	832:836	arg1	chain					850:854	the main chain	841:854	the main chain	841:854	The results showed that both PSSP and Se-PSSP contained a pyranoid polysaccharide linked by α-glycosidic bonds in the main chain.					
35227466	4	25	theme	main	845:848	arg1	chain					850:854	the main chain	841:854	the main chain	841:854	The results showed that both PSSP and Se-PSSP contained a pyranoid polysaccharide linked by α-glycosidic bonds in the main chain.					
35227466	3	26	theme	FT-IR	591:595	arg1	spectra					597:603	FT-IR spectra	591:603	FT-IR spectra	591:603	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	6	27	theme	%	1046:1046	arg1	percentages					1026:1036	molar percentages	1020:1036	molar percentages of 55.82%, 14.86% and 14.35%, respectively	1020:1079	PSSP (47.12 kDa) was mainly composed of glucose, mannose and xylose with molar percentages of 55.82%, 14.86% and 14.35%, respectively.					
35227466	8	28	theme	antioxidant	1283:1293	arg1	activity					1295:1302	antioxidant activity	1283:1302	antioxidant activity	1283:1302	Compared with PSSP, Se-PSSP showed stronger water-solubility, antioxidant activity, cytotoxicity and immunomodulatory activity than that of PSSP.					
35227466	2	29	theme	antineoplastic	438:451	arg1	characteristics					408:422	its characteristics	404:422	its characteristics	404:422	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	2	29	theme	antineoplastic	438:451	arg1	activities					464:473	antioxidant, antineoplastic and immune activities	425:473	antioxidant, antineoplastic and immune activities	425:473	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	4	30	theme	pyranoid	785:792	arg1	polysaccharide					794:807	a pyranoid polysaccharide	783:807	a pyranoid polysaccharide linked by α-glycosidic bonds in the main chain	783:854	The results showed that both PSSP and Se-PSSP contained a pyranoid polysaccharide linked by α-glycosidic bonds in the main chain.					
35227466	7	31	with	galactose	1144:1152	arg1	percentages					1165:1175	molar percentages	1159:1175	molar percentages of 26.49%, 18.76% and 18.14%, respectively	1159:1218	Se-PSSP (16.82 kDa) is mainly composed of glucose, xylose and galactose with molar percentages of 26.49%, 18.76% and 18.14%, respectively.					
35227466	2	32	theme	L	339:339	arg1	fraction					303:310	A purified polysaccharide fraction	277:310	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP)	277:347	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	8	33	theme	immunomodulatory	1322:1337	arg1	activity					1339:1346	immunomodulatory activity	1322:1346	immunomodulatory activity	1322:1346	Compared with PSSP, Se-PSSP showed stronger water-solubility, antioxidant activity, cytotoxicity and immunomodulatory activity than that of PSSP.					
35227466	2	34	theme	antioxidant	425:435	arg1	characteristics					408:422	its characteristics	404:422	its characteristics	404:422	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	2	34	theme	antioxidant	425:435	arg1	activities					464:473	antioxidant, antineoplastic and immune activities	425:473	antioxidant, antineoplastic and immune activities	425:473	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	2	35	theme	sagittifolia	326:337	arg1	L					339:339	Sagittaria sagittifolia L	315:339	Sagittaria sagittifolia L. (PSSP)	315:347	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	2	35	theme	sagittifolia	326:337	arg1	PSSP					343:346	PSSP	343:346	PSSP	343:346	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	9	36	from	agents	1533:1538	arg1	field					1547:1551	the field	1543:1551	the field of functional foods and medicine industry	1543:1593	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	1	37	theme	solubility	222:231	arg1	responses					266:274	polysaccharides solubility, antioxidant, anticancer, immune responses	206:274	polysaccharides solubility, antioxidant, anticancer, immune responses	206:274	This study proposed an optimal way to supplement organic selenium, boost polysaccharides solubility, antioxidant, anticancer, immune responses.					
35227466	7	38	theme	%	1204:1204	arg1	percentages					1165:1175	molar percentages	1159:1175	molar percentages of 26.49%, 18.76% and 18.14%, respectively	1159:1218	Se-PSSP (16.82 kDa) is mainly composed of glucose, xylose and galactose with molar percentages of 26.49%, 18.76% and 18.14%, respectively.					
35227466	3	39	theme	scanning	657:664	arg1	microscope					675:684	scanning electron microscope	657:684	scanning electron microscope (SEM)	657:690	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	3	39	theme	scanning	657:664	arg1	SEM					687:689	SEM	687:689	SEM	687:689	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	6	40	with	mannose	996:1002	arg1	percentages					1026:1036	molar percentages	1020:1036	molar percentages of 55.82%, 14.86% and 14.35%, respectively	1020:1079	PSSP (47.12 kDa) was mainly composed of glucose, mannose and xylose with molar percentages of 55.82%, 14.86% and 14.35%, respectively.					
35227466	6	41	theme	molar	1020:1024	arg1	percentages					1026:1036	molar percentages	1020:1036	molar percentages of 55.82%, 14.86% and 14.35%, respectively	1020:1079	PSSP (47.12 kDa) was mainly composed of glucose, mannose and xylose with molar percentages of 55.82%, 14.86% and 14.35%, respectively.					
35227466	9	42	theme	foods	1567:1571	arg1	field					1547:1551	the field	1543:1551	the field of functional foods and medicine industry	1543:1593	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	7	43	with	xylose	1133:1138	arg1	percentages					1165:1175	molar percentages	1159:1175	molar percentages of 26.49%, 18.76% and 18.14%, respectively	1159:1218	Se-PSSP (16.82 kDa) is mainly composed of glucose, xylose and galactose with molar percentages of 26.49%, 18.76% and 18.14%, respectively.					
35227466	9	44	theme	promising	1409:1417	arg1	Se-supplement					1425:1437	a promising novel Se-supplement	1407:1437	a promising novel Se-supplement	1407:1437	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	9	44	theme	promising	1409:1417	arg1	Se-PSSP					1396:1402	Se-PSSP	1396:1402	Se-PSSP	1396:1402	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	4	45	theme	α-glycosidic	819:830	arg1	bonds					832:836	α-glycosidic bonds	819:836	α-glycosidic bonds in the main chain	819:854	The results showed that both PSSP and Se-PSSP contained a pyranoid polysaccharide linked by α-glycosidic bonds in the main chain.					
35227466	6	46	theme	%	1065:1065	arg1	percentages					1026:1036	molar percentages	1020:1036	molar percentages of 55.82%, 14.86% and 14.35%, respectively	1020:1079	PSSP (47.12 kDa) was mainly composed of glucose, mannose and xylose with molar percentages of 55.82%, 14.86% and 14.35%, respectively.					
35227466	9	47	theme	novel	1419:1423	arg1	Se-supplement					1425:1437	a promising novel Se-supplement	1407:1437	a promising novel Se-supplement	1407:1437	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	9	47	theme	novel	1419:1423	arg1	Se-PSSP					1396:1402	Se-PSSP	1396:1402	Se-PSSP	1396:1402	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	0	48	theme	immune	50:55	arg1	activities					57:66	immune activities	50:66	immune activities	50:66	Characterization, antioxidant, antineoplastic and immune activities of selenium modified Sagittaria sagittifolia L. polysaccharides.					
35227466	9	49	theme	excellent	1463:1471	arg1	antioxidant					1483:1493	an excellent potential antioxidant	1460:1493	an excellent potential antioxidant	1460:1493	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	3	50	theme	NMR	606:608	arg1	spectra					610:616	NMR spectra	606:616	NMR spectra	606:616	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	8	51	dep	stronger	1256:1263	arg1	activity					1339:1346	immunomodulatory activity	1322:1346	immunomodulatory activity	1322:1346	Compared with PSSP, Se-PSSP showed stronger water-solubility, antioxidant activity, cytotoxicity and immunomodulatory activity than that of PSSP.					
35227466	8	51	dep	stronger	1256:1263	arg1	water-solubility					1265:1280	water-solubility	1265:1280	water-solubility	1265:1280	Compared with PSSP, Se-PSSP showed stronger water-solubility, antioxidant activity, cytotoxicity and immunomodulatory activity than that of PSSP.					
35227466	8	51	dep	stronger	1256:1263	arg1	cytotoxicity					1305:1316	cytotoxicity	1305:1316	cytotoxicity	1305:1316	Compared with PSSP, Se-PSSP showed stronger water-solubility, antioxidant activity, cytotoxicity and immunomodulatory activity than that of PSSP.					
35227466	8	51	dep	stronger	1256:1263	arg1	activity					1295:1302	antioxidant activity	1283:1302	antioxidant activity	1283:1302	Compared with PSSP, Se-PSSP showed stronger water-solubility, antioxidant activity, cytotoxicity and immunomodulatory activity than that of PSSP.					
35227466	5	52	theme	amorphous	892:900	arg1	morphology					902:911	amorphous morphology	892:911	amorphous morphology	892:911	In addition, PSSP and Se-PSSP were amorphous morphology without three-helix conformation.					
35227466	3	53	theme	diffraction	625:635	arg1	XRD					651:653	XRD	651:653	XRD	651:653	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	3	53	theme	diffraction	625:635	arg1	spectroscopy					637:648	X-ray diffraction spectroscopy	619:648	X-ray diffraction spectroscopy (XRD)	619:654	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	2	54	theme	purified	279:286	arg1	fraction					303:310	A purified polysaccharide fraction	277:310	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP)	277:347	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	7	55	theme	%	1193:1193	arg1	percentages					1165:1175	molar percentages	1159:1175	molar percentages of 26.49%, 18.76% and 18.14%, respectively	1159:1218	Se-PSSP (16.82 kDa) is mainly composed of glucose, xylose and galactose with molar percentages of 26.49%, 18.76% and 18.14%, respectively.					
35227466	3	56	theme	electron	666:673	arg1	microscope					675:684	scanning electron microscope	657:684	scanning electron microscope (SEM)	657:690	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	3	56	theme	electron	666:673	arg1	SEM					687:689	SEM	687:689	SEM	687:689	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	1	57	dep	proposed	144:151	arg1	boost					200:204	boost	200:204	boost polysaccharides solubility, antioxidant, anticancer, immune responses	200:274	This study proposed an optimal way to supplement organic selenium, boost polysaccharides solubility, antioxidant, anticancer, immune responses.					
35227466	9	58	from	antineoplastic	1496:1509	arg1	field					1547:1551	the field	1543:1551	the field of functional foods and medicine industry	1543:1593	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	0	59	theme	selenium	71:78	arg1	antioxidant					18:28	antioxidant	18:28	antioxidant	18:28	Characterization, antioxidant, antineoplastic and immune activities of selenium modified Sagittaria sagittifolia L. polysaccharides.					
35227466	0	59	theme	selenium	71:78	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, antioxidant, antineoplastic and immune activities of selenium modified Sagittaria sagittifolia L. polysaccharides.					
35227466	0	59	theme	selenium	71:78	arg1	antineoplastic					31:44	antineoplastic	31:44	antineoplastic	31:44	Characterization, antioxidant, antineoplastic and immune activities of selenium modified Sagittaria sagittifolia L. polysaccharides.					
35227466	0	59	theme	selenium	71:78	arg1	activities					57:66	immune activities	50:66	immune activities	50:66	Characterization, antioxidant, antineoplastic and immune activities of selenium modified Sagittaria sagittifolia L. polysaccharides.					
35227466	3	60	theme	X-ray	619:623	arg1	XRD					651:653	XRD	651:653	XRD	651:653	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	3	60	theme	X-ray	619:623	arg1	spectroscopy					637:648	X-ray diffraction spectroscopy	619:648	X-ray diffraction spectroscopy (XRD)	619:654	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	9	61	from	antioxidant	1483:1493	arg1	field					1547:1551	the field	1543:1551	the field of functional foods and medicine industry	1543:1593	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	1	62	theme	optimal	156:162	arg1	way					164:166	an optimal way	153:166	an optimal way to supplement organic selenium	153:197	This study proposed an optimal way to supplement organic selenium, boost polysaccharides solubility, antioxidant, anticancer, immune responses.					
35227466	9	63	theme	potential	1473:1481	arg1	antioxidant					1483:1493	an excellent potential antioxidant	1460:1493	an excellent potential antioxidant	1460:1493	These results suggested that Se-PSSP is a promising novel Se-supplement and may be served as an excellent potential antioxidant, antineoplastic, and immunomodulatory agents in the field of functional foods and medicine industry.					
35227466	3	64	theme	atomic	696:701	arg1	AFM					721:723	AFM	721:723	AFM	721:723	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	3	64	theme	atomic	696:701	arg1	microscope					709:718	atomic force microscope	696:718	atomic force microscope (AFM)	696:724	The structure and the monosaccharide composition were determined by means of UV-visible spectrometry, FT-IR spectra, NMR spectra, X-ray diffraction spectroscopy (XRD), scanning electron microscope (SEM) and atomic force microscope (AFM).					
35227466	1	65	dep	solubility	222:231	arg1	anticancer					247:256	anticancer	247:256	anticancer	247:256	This study proposed an optimal way to supplement organic selenium, boost polysaccharides solubility, antioxidant, anticancer, immune responses.					
35227466	1	65	dep	solubility	222:231	arg1	antioxidant					234:244	antioxidant	234:244	antioxidant	234:244	This study proposed an optimal way to supplement organic selenium, boost polysaccharides solubility, antioxidant, anticancer, immune responses.					
35227466	2	66	mod	modified	366:373	arg3	Se-PSSP					390:396	Se-PSSP	390:396	Se-PSSP	390:396	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	2	66	mod	modified	366:373	arg1	fraction					303:310	A purified polysaccharide fraction	277:310	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP)	277:347	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	2	66	mod	modified	366:373	arg3	selenium					380:387	selenium	380:387	selenium (Se-PSSP)	380:397	A purified polysaccharide fraction of Sagittaria sagittifolia L. (PSSP) was successfully modified with selenium (Se-PSSP), and its characteristics, antioxidant, antineoplastic and immune activities were studied.					
35227466	1	67	theme	immune	259:264	arg1	responses					266:274	polysaccharides solubility, antioxidant, anticancer, immune responses	206:274	polysaccharides solubility, antioxidant, anticancer, immune responses	206:274	This study proposed an optimal way to supplement organic selenium, boost polysaccharides solubility, antioxidant, anticancer, immune responses.					
34792073	0	0	theme	mixed-mode	94:103	arg1	application					110:120	mixed-mode HPLC application	94:120	mixed-mode HPLC application	94:120	Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application.					
34792073	3	1	from	plates	643:648	arg1	mode					627:630	HILIC mode	621:630	HILIC mode	621:630	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	3	1	from	plates	643:648	arg1	mode					663:666	IEC mode	659:666	IEC mode	659:666	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	0	2	from	coating	15:21	arg1	silica					83:88	silica	83:88	silica	83:88	Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application.					
34792073	2	3	theme	hyaluronan	331:340	arg1	acid					342:345	hyaluronan acid	331:345	hyaluronan acid	331:345	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	2	3	theme	hyaluronan	331:340	arg1	polysaccharides					314:328	Two natural polysaccharides	302:328	Two natural polysaccharides (hyaluronan acid and chitosan)	302:359	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	0	4	theme	HPLC	105:108	arg1	application					110:120	mixed-mode HPLC application	94:120	mixed-mode HPLC application	94:120	Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application.					
34792073	3	5	theme	great	685:689	arg1	potential					691:699	great potential	685:699	great potential for separating polar and charged samples	685:740	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	2	6	theme	silica	393:398	arg1	surface					400:406	the silica surface	389:406	the silica surface	389:406	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	3	7	dep	reached	590:596	arg1	indicates					675:683	indicates	675:683	indicates great potential for separating polar and charged samples	675:740	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	3	8	theme	column	543:548	arg1	efficiency					550:559	The column efficiency	539:559	The column efficiency of the SiO2@(HA-CS)12 column	539:588	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	3	9	from	plates	605:610	arg1	mode					627:630	HILIC mode	621:630	HILIC mode	621:630	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	3	9	from	plates	605:610	arg1	mode					663:666	IEC mode	659:666	IEC mode	659:666	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	2	10	dep	polysaccharides	314:328	arg1	acid					342:345	hyaluronan acid	331:345	hyaluronan acid	331:345	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	2	10	dep	polysaccharides	314:328	arg1	chitosan					351:358	chitosan	351:358	chitosan	351:358	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	2	10	dep	polysaccharides	314:328	arg1	polysaccharides					314:328	Two natural polysaccharides	302:328	Two natural polysaccharides (hyaluronan acid and chitosan)	302:359	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	0	11	from	cross-linking	36:48	arg1	silica					83:88	silica	83:88	silica	83:88	Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application.					
34792073	3	12	theme	@	572:572	arg1	column					583:588	the SiO2@(HA-CS)12 column	564:588	the SiO2@(HA-CS)12 column	564:588	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	1	13	theme	novel	200:204	arg1	HA-CS					262:266	SiO2@(HA-CS)12	256:269	SiO2@(HA-CS)12	256:269	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	1	13	theme	novel	200:204	arg1	phase					249:253	a novel polysaccharide-silica composite stationary phase	198:253	a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12)	198:270	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	0	14	theme	Layer-by-layer	0:13	arg1	coating					15:21	Layer-by-layer coating	0:21	Layer-by-layer coating	0:21	Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application.					
34792073	2	15	theme	natural	306:312	arg1	acid					342:345	hyaluronan acid	331:345	hyaluronan acid	331:345	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	2	15	theme	natural	306:312	arg1	chitosan					351:358	chitosan	351:358	chitosan	351:358	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	2	15	theme	natural	306:312	arg1	polysaccharides					314:328	Two natural polysaccharides	302:328	Two natural polysaccharides (hyaluronan acid and chitosan)	302:359	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	1	16	theme	polysaccharide-silica	206:226	arg1	HA-CS					262:266	SiO2@(HA-CS)12	256:269	SiO2@(HA-CS)12	256:269	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	1	16	theme	polysaccharide-silica	206:226	arg1	phase					249:253	a novel polysaccharide-silica composite stationary phase	198:253	a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12)	198:270	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	0	17	theme	chemical	27:34	arg1	cross-linking					36:48	chemical cross-linking	27:48	chemical cross-linking	27:48	Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application.					
34792073	3	18	theme	polar	716:720	arg1	samples					734:740	polar and charged samples	716:740	polar and charged samples	716:740	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	2	19	theme	layer-by-layer	416:429	arg1	technique					440:448	a layer-by-layer assembly technique	414:448	a layer-by-layer assembly technique	414:448	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	2	20	theme	assembly	431:438	arg1	technique					440:448	a layer-by-layer assembly technique	414:448	a layer-by-layer assembly technique	414:448	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	3	21	theme	HILIC	621:625	arg1	mode					627:630	HILIC mode	621:630	HILIC mode	621:630	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	3	22	theme	SiO2	568:571	arg1	column					583:588	the SiO2@(HA-CS)12 column	564:588	the SiO2@(HA-CS)12 column	564:588	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	1	23	theme	stationary	238:247	arg1	HA-CS					262:266	SiO2@(HA-CS)12	256:269	SiO2@(HA-CS)12	256:269	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	1	23	theme	stationary	238:247	arg1	phase					249:253	a novel polysaccharide-silica composite stationary phase	198:253	a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12)	198:270	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	2	24	theme	polysaccharide	464:477	arg1	shell					479:483	then the polysaccharide shell	455:483	then the polysaccharide shell	455:483	Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability.					
34792073	3	25	theme	column	583:588	arg1	efficiency					550:559	The column efficiency	539:559	The column efficiency of the SiO2@(HA-CS)12 column	539:588	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	0	26	theme	polysaccharides	64:78	arg1	coating					15:21	Layer-by-layer coating	0:21	Layer-by-layer coating	0:21	Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application.					
34792073	0	26	theme	polysaccharides	64:78	arg1	cross-linking					36:48	chemical cross-linking	27:48	chemical cross-linking	27:48	Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application.					
34792073	3	27	theme	HA-CS	574:578	arg1	column					583:588	the SiO2@(HA-CS)12 column	564:588	the SiO2@(HA-CS)12 column	564:588	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	1	28	theme	facile	125:130	arg1	method					175:180	A facile, controllable and environmentally friendly method	123:180	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12)	123:270	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	0	29	theme	multilayer	53:62	arg1	polysaccharides					64:78	multilayer polysaccharides	53:78	multilayer polysaccharides	53:78	Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application.					
34792073	3	30	theme	charged	726:732	arg1	samples					734:740	polar and charged samples	716:740	polar and charged samples	716:740	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	1	31	theme	controllable	133:144	arg1	method					175:180	A facile, controllable and environmentally friendly method	123:180	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12)	123:270	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	1	32	theme	SiO2	256:259	arg1	HA-CS					262:266	SiO2@(HA-CS)12	256:269	SiO2@(HA-CS)12	256:269	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	1	32	theme	SiO2	256:259	arg1	phase					249:253	a novel polysaccharide-silica composite stationary phase	198:253	a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12)	198:270	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	1	33	theme	@	260:260	arg1	HA-CS					262:266	SiO2@(HA-CS)12	256:269	SiO2@(HA-CS)12	256:269	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	1	33	theme	@	260:260	arg1	phase					249:253	a novel polysaccharide-silica composite stationary phase	198:253	a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12)	198:270	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	1	34	theme	composite	228:236	arg1	HA-CS					262:266	SiO2@(HA-CS)12	256:269	SiO2@(HA-CS)12	256:269	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	1	34	theme	composite	228:236	arg1	phase					249:253	a novel polysaccharide-silica composite stationary phase	198:253	a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12)	198:270	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	1	35	theme	environmentally	150:164	arg1	method					175:180	A facile, controllable and environmentally friendly method	123:180	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12)	123:270	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
34792073	3	36	theme	IEC	659:661	arg1	mode					663:666	IEC mode	659:666	IEC mode	659:666	The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.					
34792073	1	37	theme	friendly	166:173	arg1	method					175:180	A facile, controllable and environmentally friendly method	123:180	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12)	123:270	A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report.					
33913112	0	0	theme	chitosan/alginate-based	75:97	arg1	composites					99:108	chitosan/alginate-based composites	75:108	chitosan/alginate-based composites	75:108	Synergistic effect of the activated carbon addition from leather wastes in chitosan/alginate-based composites.					
33913112	6	1	theme	textile	830:836	arg1	effluent					838:845	real textile effluent	825:845	real textile effluent	825:845	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	2	from	%	951:951	arg1	color					978:982	color	978:982	color	978:982	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	2	from	%	951:951	arg1	COD					943:945	COD	943:945	COD	943:945	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	2	from	%	951:951	arg1	turbidity					956:964	turbidity	956:964	turbidity	956:964	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	2	from	%	951:951	arg1	demand					935:940	the chemical oxygen demand	915:940	the chemical oxygen demand (COD)	915:946	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	4	3	theme	adsorption	634:643	arg1	capacity					645:652	the adsorption capacity	630:652	the adsorption capacity 1.2 times higher than expected	630:683	The synergy was due to the reduction in steric hindrance, with the adsorption capacity 1.2 times higher than expected.					
33913112	2	4	theme	Blue	396:399	arg1	Brilliant					386:394	the textile dye Remazol Brilliant Blue R (RBBR)	362:408	the textile dye Remazol Brilliant Blue R (RBBR)	362:408	The performance of these composites was verified through the adsorption of the textile dye Remazol Brilliant Blue R (RBBR).					
33913112	6	5	theme	real	825:828	arg1	effluent					838:845	real textile effluent	825:845	real textile effluent	825:845	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	6	theme	30	777:778	arg1	%					779:779	%	779:779	%	779:779	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	1	7	theme	tannery	265:271	arg1	ACTW					280:283	ACTW	280:283	ACTW	280:283	In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW).					
33913112	1	7	theme	tannery	265:271	arg1	waste					273:277	tannery waste	265:277	tannery waste (ACTW)	265:284	In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW).					
33913112	6	8	from	%	910:910	arg1	color					978:982	color	978:982	color	978:982	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	8	from	%	910:910	arg1	COD					943:945	COD	943:945	COD	943:945	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	8	from	%	910:910	arg1	turbidity					956:964	turbidity	956:964	turbidity	956:964	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	8	from	%	910:910	arg1	demand					935:940	the chemical oxygen demand	915:940	the chemical oxygen demand (COD)	915:946	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	9	theme	oxygen	887:892	arg1	BOD					902:904	BOD	902:904	BOD	902:904	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	9	theme	oxygen	887:892	arg1	demand					894:899	the biochemical oxygen demand	871:899	the biochemical oxygen demand (BOD)	871:905	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	3	10	dep	area	494:497	arg1	m2/g					512:515	m2/g	512:515	m2/g	512:515	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	6	11	theme	chemical	919:926	arg1	COD					943:945	COD	943:945	COD	943:945	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	11	theme	chemical	919:926	arg1	demand					935:940	the chemical oxygen demand	915:940	the chemical oxygen demand (COD)	915:946	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	1	12	theme	gelation	176:183	arg1	method					185:190	the gelation method	172:190	the gelation method	172:190	In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW).					
33913112	5	13	theme	sodium	720:725	arg1	hydroxide					727:735	sodium hydroxide	720:735	sodium hydroxide for 10 cycles	720:749	The material was regenerated with sodium hydroxide for 10 cycles.					
33913112	6	14	dep	applied	797:803	arg1	%					951:951	78%	949:951	78% in turbidity	949:964	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	14	dep	applied	797:803	arg1	%					973:973	67%	971:973	67% in color	971:982	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	14	dep	applied	797:803	arg1	%					910:910	39%	908:910	39% in the chemical oxygen demand (COD)	908:946	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	2	15	theme	Brilliant	386:394	arg1	adsorption					348:357	the adsorption	344:357	the adsorption of the textile dye Remazol Brilliant Blue R (RBBR)	344:408	The performance of these composites was verified through the adsorption of the textile dye Remazol Brilliant Blue R (RBBR).					
33913112	2	16	theme	composites	312:321	arg1	performance					291:301	The performance	287:301	The performance of these composites	287:321	The performance of these composites was verified through the adsorption of the textile dye Remazol Brilliant Blue R (RBBR).					
33913112	4	17	from	reduction	594:602	arg1	hindrance					614:622	steric hindrance	607:622	steric hindrance	607:622	The synergy was due to the reduction in steric hindrance, with the adsorption capacity 1.2 times higher than expected.					
33913112	2	18	theme	Remazol	378:384	arg1	Brilliant					386:394	the textile dye Remazol Brilliant Blue R (RBBR)	362:408	the textile dye Remazol Brilliant Blue R (RBBR)	362:408	The performance of these composites was verified through the adsorption of the textile dye Remazol Brilliant Blue R (RBBR).					
33913112	0	19	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of the activated carbon addition from leather	0:63	Synergistic effect of the activated carbon addition from leather wastes in chitosan/alginate-based composites.					
33913112	6	20	theme	biochemical	875:885	arg1	BOD					902:904	BOD	902:904	BOD	902:904	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	20	theme	biochemical	875:885	arg1	demand					894:899	the biochemical oxygen demand	871:899	the biochemical oxygen demand (BOD)	871:905	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	3	21	theme	maximum	522:528	arg1	capacity					541:548	the maximum adsorption capacity	518:548	the maximum adsorption capacity	518:548	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	3	21	theme	maximum	522:528	arg1	mg/g					561:564	300.96 mg/g	554:564	300.96 mg/g	554:564	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	2	22	theme	dye	374:376	arg1	Brilliant					386:394	the textile dye Remazol Brilliant Blue R (RBBR)	362:408	the textile dye Remazol Brilliant Blue R (RBBR)	362:408	The performance of these composites was verified through the adsorption of the textile dye Remazol Brilliant Blue R (RBBR).					
33913112	6	23	from	%	973:973	arg1	color					978:982	color	978:982	color	978:982	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	23	from	%	973:973	arg1	COD					943:945	COD	943:945	COD	943:945	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	23	from	%	973:973	arg1	turbidity					956:964	turbidity	956:964	turbidity	956:964	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	23	from	%	973:973	arg1	demand					935:940	the chemical oxygen demand	915:940	the chemical oxygen demand (COD)	915:946	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	24	theme	oxygen	928:933	arg1	COD					943:945	COD	943:945	COD	943:945	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	24	theme	oxygen	928:933	arg1	demand					935:940	the chemical oxygen demand	915:940	the chemical oxygen demand (COD)	915:946	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	4	25	dep	times	658:662	arg1	higher					664:669	higher	664:669	higher	664:669	The synergy was due to the reduction in steric hindrance, with the adsorption capacity 1.2 times higher than expected.					
33913112	6	26	from	reductions	857:866	arg1	BOD					902:904	BOD	902:904	BOD	902:904	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	26	from	reductions	857:866	arg1	demand					894:899	the biochemical oxygen demand	871:899	the biochemical oxygen demand (BOD)	871:905	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	4	27	dep	capacity	645:652	arg1	times					658:662	times	658:662	times	658:662	The synergy was due to the reduction in steric hindrance, with the adsorption capacity 1.2 times higher than expected.					
33913112	3	28	theme	specific	477:484	arg1	area					494:497	a specific surface area	475:497	a specific surface area up to 45.584 m2/g	475:515	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	0	29	theme	carbon	36:41	arg1	addition					43:50	the activated carbon addition	22:50	the activated carbon addition	22:50	Synergistic effect of the activated carbon addition from leather wastes in chitosan/alginate-based composites.					
33913112	4	30	theme	steric	607:612	arg1	hindrance					614:622	steric hindrance	607:622	steric hindrance	607:622	The synergy was due to the reduction in steric hindrance, with the adsorption capacity 1.2 times higher than expected.					
33913112	1	31	theme	different	213:221	arg1	carbon					244:249	activated carbon	234:249	activated carbon	234:249	In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW).					
33913112	1	31	theme	different	213:221	arg1	amounts					223:229	different amounts	213:229	different amounts of activated carbon produced from tannery waste (ACTW)	213:284	In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW).					
33913112	0	32	theme	activated	26:34	arg1	addition					43:50	the activated carbon addition	22:50	the activated carbon addition	22:50	Synergistic effect of the activated carbon addition from leather wastes in chitosan/alginate-based composites.					
33913112	6	33	theme	%	855:855	arg1	reductions					857:866	30% reductions	853:866	30% reductions in the biochemical oxygen demand (BOD)	853:905	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	1	34	theme	amounts	223:229	arg1	addition					201:208	the addition	197:208	the addition of different amounts of activated carbon produced from tannery waste (ACTW)	197:284	In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW).					
33913112	6	35	theme	30	853:854	arg1	%					855:855	%	855:855	%	855:855	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	0	36	from	leather	57:63	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of the activated carbon addition from leather	0:63	Synergistic effect of the activated carbon addition from leather wastes in chitosan/alginate-based composites.					
33913112	3	37	theme	adsorption	530:539	arg1	capacity					541:548	the maximum adsorption capacity	518:548	the maximum adsorption capacity	518:548	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	3	37	theme	adsorption	530:539	arg1	mg/g					561:564	300.96 mg/g	554:564	300.96 mg/g	554:564	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	3	38	theme	ACTW	464:467	arg1	addition					452:459	the addition	448:459	the addition of ACTW	448:467	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	0	39	theme	addition	43:50	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of the activated carbon addition from leather	0:63	Synergistic effect of the activated carbon addition from leather wastes in chitosan/alginate-based composites.					
33913112	3	40	theme	synergistic	413:423	arg1	effect					425:430	A synergistic effect	411:430	A synergistic effect	411:430	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	4	41	theme	1.2	654:656	arg1	times					658:662	times	658:662	times	658:662	The synergy was due to the reduction in steric hindrance, with the adsorption capacity 1.2 times higher than expected.					
33913112	6	42	theme	%	779:779	arg1	AC30					787:790	AC30	787:790	AC30	787:790	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	42	theme	%	779:779	arg1	ACTW					781:784	30% ACTW	777:784	30% ACTW (AC30)	777:791	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	1	43	theme	chitosan/alginate	125:141	arg1	composites					143:152	chitosan/alginate composites	125:152	chitosan/alginate composites	125:152	In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW).					
33913112	4	44	with	due	583:585	arg1	capacity					645:652	the adsorption capacity	630:652	the adsorption capacity 1.2 times higher than expected	630:683	The synergy was due to the reduction in steric hindrance, with the adsorption capacity 1.2 times higher than expected.					
33913112	6	45	contain	containing	766:775	arg2	AC30					787:790	AC30	787:790	AC30	787:790	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	45	contain	containing	766:775	arg2	ACTW					781:784	30% ACTW	777:784	30% ACTW (AC30)	777:791	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	6	45	contain	containing	766:775	arg1	composite					756:764	The composite	752:764	The composite containing 30% ACTW (AC30)	752:791	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	3	46	dep	45.584	505:510	arg1	to					502:503	to	502:503	to	502:503	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	1	47	theme	activated	234:242	arg1	carbon					244:249	activated carbon	234:249	activated carbon	234:249	In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW).					
33913112	3	48	with	mg/g	561:564	arg1	area					494:497	a specific surface area	475:497	a specific surface area up to 45.584 m2/g	475:515	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	3	49	dep	m2/g	512:515	arg1	45.584					505:510	45.584	505:510	45.584	505:510	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33913112	2	50	theme	textile	366:372	arg1	Brilliant					386:394	the textile dye Remazol Brilliant Blue R (RBBR)	362:408	the textile dye Remazol Brilliant Blue R (RBBR)	362:408	The performance of these composites was verified through the adsorption of the textile dye Remazol Brilliant Blue R (RBBR).					
33913112	1	51	theme	carbon	244:249	arg1	carbon					244:249	activated carbon	234:249	activated carbon	234:249	In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW).					
33913112	1	51	theme	carbon	244:249	arg1	amounts					223:229	different amounts	213:229	different amounts of activated carbon produced from tannery waste (ACTW)	213:284	In this work, chitosan/alginate composites were developed by the gelation method with the addition of different amounts of activated carbon produced from tannery waste (ACTW).					
33913112	6	52	theme	effluent	838:845	arg1	treatment					812:820	the treatment	808:820	the treatment of real textile effluent	808:845	The composite containing 30% ACTW (AC30) was applied in the treatment of real textile effluent, with 30% reductions in the biochemical oxygen demand (BOD), 39% in the chemical oxygen demand (COD), 78% in turbidity, and 67% in color.					
33913112	2	53	dep	Brilliant	386:394	arg1	RBBR					404:407	RBBR	404:407	RBBR	404:407	The performance of these composites was verified through the adsorption of the textile dye Remazol Brilliant Blue R (RBBR).					
33913112	2	53	dep	Brilliant	386:394	arg1	R					401:401	R	401:401	the textile dye Remazol Brilliant Blue R (RBBR)	362:408	The performance of these composites was verified through the adsorption of the textile dye Remazol Brilliant Blue R (RBBR).					
33913112	3	54	theme	surface	486:492	arg1	area					494:497	a specific surface area	475:497	a specific surface area up to 45.584 m2/g	475:515	A synergistic effect was observed by the addition of ACTW; with a specific surface area up to 45.584 m2/g, the maximum adsorption capacity was 300.96 mg/g.					
33113670	13	0	theme	degradation	2102:2112	arg1	process					2114:2120	the degradation process	2098:2120	the degradation process	2098:2120	And the content of some small molecules was briefly increased during the degradation process.					
33113670	5	1	from	Changes	717:723	arg1	process					800:806	the degradation process	784:806	the degradation process	784:806	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	5	1	from	Changes	717:723	arg1	EPSs					750:753	EPSs	750:753	EPSs	750:753	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	5	1	from	Changes	717:723	arg1	communities					769:779	microbial communities	759:779	microbial communities	759:779	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	8	2	theme	C3	1293:1294	arg1	changes					1248:1254	The changes	1244:1254	The changes of humic-like C1 and tryptophan-like C3 in EPSs	1244:1302	The changes of humic-like C1 and tryptophan-like C3 in EPSs were significantly correlated to the variations of microbial community composition and diversity.					
33113670	3	3	dep	cyanobacteria	580:592	arg1	bloom					594:598	bloom	594:598	bloom	594:598	A better understanding of the dynamic process not only give insight into the carbon cycling in the phycosphere, but also provide a new approach for controlling the cyanobacteria bloom.					
33113670	7	4	theme	different	1112:1120	arg1	activities					1133:1142	different hydrolytic activities	1112:1142	different hydrolytic activities	1112:1142	Size-fractionated microorganisms exhibited different hydrolytic activities when interacting with macromolecules, but they did not present different phylogenetic compositions.					
33113670	1	5	theme	different	195:203	arg1	components					213:222	different organic components	195:222	different organic components	195:222	Extracellular polymeric substances (EPSs), which composed of different organic components, play an important role in the formation of mucilaginous cyanobacterial bloom.					
33113670	10	6	theme	maters	1689:1694	arg1	changes					1670:1676	the changes	1666:1676	the changes of organic maters through the humification process	1666:1727	Betaproteobacteria, Firmicute, Alphaproteobacteria, Sphingobacteria and Actinobacter were significantly correlated with the changes of organic maters through the humification process.					
33113670	5	7	from	components	736:745	arg1	EPSs					750:753	EPSs	750:753	EPSs	750:753	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	5	7	from	components	736:745	arg1	communities					769:779	microbial communities	759:779	microbial communities	759:779	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	8	8	theme	humic-like	1259:1268	arg1	C1					1270:1271	humic-like C1	1259:1271	humic-like C1	1259:1271	The changes of humic-like C1 and tryptophan-like C3 in EPSs were significantly correlated to the variations of microbial community composition and diversity.					
33113670	6	9	theme	organic	951:957	arg1	components					959:968	organic components	951:968	organic components in the degradation process	951:995	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	5	10	theme	matrix	869:874	arg1	EEM					877:879	emission matrix (EEM) and Illumina sequencing	860:904	EEM	877:879	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	9	11	theme	Tightly-bound	1402:1414	arg1	EPSs					1416:1419	Tightly-bound EPSs	1402:1419	Tightly-bound EPSs (TB-EPSs)	1402:1429	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	9	11	theme	Tightly-bound	1402:1414	arg1	TB-EPSs					1422:1428	TB-EPSs	1422:1428	TB-EPSs	1422:1428	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	1	12	theme	Extracellular	134:146	arg1	EPSs					170:173	EPSs	170:173	EPSs	170:173	Extracellular polymeric substances (EPSs), which composed of different organic components, play an important role in the formation of mucilaginous cyanobacterial bloom.					
33113670	1	12	theme	Extracellular	134:146	arg1	substances					158:167	Extracellular polymeric substances	134:167	Extracellular polymeric substances (EPSs)	134:174	Extracellular polymeric substances (EPSs), which composed of different organic components, play an important role in the formation of mucilaginous cyanobacterial bloom.					
33113670	8	13	theme	microbial	1355:1363	arg1	composition					1375:1385	microbial community composition	1355:1385	microbial community composition	1355:1385	The changes of humic-like C1 and tryptophan-like C3 in EPSs were significantly correlated to the variations of microbial community composition and diversity.					
33113670	13	14	theme	small	2053:2057	arg1	molecules					2059:2067	some small molecules	2048:2067	some small molecules	2048:2067	And the content of some small molecules was briefly increased during the degradation process.					
33113670	11	15	theme	organized	1847:1855	arg1	community					1867:1875	a functional organized microbial community	1834:1875	a functional organized microbial community	1834:1875	Meanwhile, loosely-bound EPSs (LB-EPSs), which composed of more macromolecules, were more affiliated to a functional organized microbial community.					
33113670	5	16	from	EPSs	750:753	arg1	Changes					717:723	Changes	717:723	Changes of organic components in EPSs and microbial communities in the degradation process	717:806	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	8	17	theme	composition	1375:1385	arg1	variations					1341:1350	the variations	1337:1350	the variations of microbial community composition and diversity	1337:1399	The changes of humic-like C1 and tryptophan-like C3 in EPSs were significantly correlated to the variations of microbial community composition and diversity.					
33113670	0	18	theme	substance	80:88	arg1	degradation					96:106	extracellular polymeric substance (EPS) degradation	56:106	extracellular polymeric substance (EPS) degradation of cyanobacterial blooms	56:131	Characteristics and bacterial community dynamics during extracellular polymeric substance (EPS) degradation of cyanobacterial blooms.					
33113670	5	19	theme	Fluorescence	832:843	arg1	excitation					845:854	Fluorescence excitation	832:854	Fluorescence excitation	832:854	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	4	20	theme	particle	687:694	arg1	microorganisms					701:714	different particle size microorganisms	677:714	different particle size microorganisms	677:714	In this study, fractionated EPSs were prepared as a carbon source to enrich different particle size microorganisms.					
33113670	5	21	theme	organic	728:734	arg1	components					736:745	organic components	728:745	organic components in EPSs and microbial communities	728:779	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	6	22	theme	degradation	977:987	arg1	process					989:995	the degradation process	973:995	the degradation process	973:995	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	6	23	from	process	989:995	arg1	change					941:946	the change	937:946	the change of organic components in the degradation process that causes the microbial community to follow a certain succession law	937:1066	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	6	23	from	process	989:995	arg1	it					931:932	it	931:932	it	931:932	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	3	24	theme	dynamic	446:452	arg1	process					454:460	the dynamic process	442:460	the dynamic process	442:460	A better understanding of the dynamic process not only give insight into the carbon cycling in the phycosphere, but also provide a new approach for controlling the cyanobacteria bloom.					
33113670	0	25	theme	cyanobacterial	111:124	arg1	blooms					126:131	cyanobacterial blooms	111:131	cyanobacterial blooms	111:131	Characteristics and bacterial community dynamics during extracellular polymeric substance (EPS) degradation of cyanobacterial blooms.					
33113670	14	26	theme	microbial	2241:2249	arg1	communities					2251:2261	specialized microbial communities	2229:2261	specialized microbial communities	2229:2261	Therefore, in order to prevent algal bloom from reducing cellular aggregation by decreasing viscous EPSs, specialized microbial communities should be considered in the phycosphere.					
33113670	7	27	theme	phylogenetic	1217:1228	arg1	compositions					1230:1241	different phylogenetic compositions	1207:1241	different phylogenetic compositions	1207:1241	Size-fractionated microorganisms exhibited different hydrolytic activities when interacting with macromolecules, but they did not present different phylogenetic compositions.					
33113670	6	28	from	components	959:968	arg1	process					989:995	the degradation process	973:995	the degradation process	973:995	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	0	29	theme	bacterial	20:28	arg1	dynamics					40:47	bacterial community dynamics	20:47	bacterial community dynamics	20:47	Characteristics and bacterial community dynamics during extracellular polymeric substance (EPS) degradation of cyanobacterial blooms.					
33113670	9	30	used	utilized	1505:1512	arg2	TB-EPSs					1422:1428	TB-EPSs	1422:1428	TB-EPSs	1422:1428	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	9	30	used	utilized	1505:1512	arg2	EPSs					1416:1419	Tightly-bound EPSs	1402:1419	Tightly-bound EPSs (TB-EPSs)	1402:1429	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	6	31	theme	certain	1045:1051	arg1	law					1064:1066	a certain succession law	1043:1066	a certain succession law	1043:1066	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	3	32	theme	better	418:423	arg1	understanding					425:437	A better understanding	416:437	A better understanding of the dynamic process	416:460	A better understanding of the dynamic process not only give insight into the carbon cycling in the phycosphere, but also provide a new approach for controlling the cyanobacteria bloom.					
33113670	14	33	theme	viscous	2215:2221	arg1	EPSs					2223:2226	viscous EPSs	2215:2226	viscous EPSs	2215:2226	Therefore, in order to prevent algal bloom from reducing cellular aggregation by decreasing viscous EPSs, specialized microbial communities should be considered in the phycosphere.					
33113670	12	34	theme	LB-EPS	1943:1948	arg1	degradation					1950:1960	LB-EPS degradation	1943:1960	LB-EPS degradation	1943:1960	When Gammaproteobacteria and Betaproteobacteria were involved in LB-EPS degradation as indicators, the polysaccharide structures changed dramatically.					
33113670	11	35	theme	more	1789:1792	arg1	macromolecules					1794:1807	more macromolecules	1789:1807	more macromolecules	1789:1807	Meanwhile, loosely-bound EPSs (LB-EPSs), which composed of more macromolecules, were more affiliated to a functional organized microbial community.					
33113670	3	36	theme	carbon	493:498	arg1	cycling					500:506	the carbon cycling	489:506	the carbon cycling in the phycosphere	489:525	A better understanding of the dynamic process not only give insight into the carbon cycling in the phycosphere, but also provide a new approach for controlling the cyanobacteria bloom.					
33113670	0	37	theme	extracellular	56:68	arg1	degradation					96:106	extracellular polymeric substance (EPS) degradation	56:106	extracellular polymeric substance (EPS) degradation of cyanobacterial blooms	56:131	Characteristics and bacterial community dynamics during extracellular polymeric substance (EPS) degradation of cyanobacterial blooms.					
33113670	14	38	theme	cellular	2180:2187	arg1	aggregation					2189:2199	cellular aggregation	2180:2199	cellular aggregation	2180:2199	Therefore, in order to prevent algal bloom from reducing cellular aggregation by decreasing viscous EPSs, specialized microbial communities should be considered in the phycosphere.					
33113670	2	39	theme	microbial	333:341	arg1	coupling					353:360	microbial community coupling	333:360	microbial community coupling	333:360	However, how the phylogeny of microbial community coupling with the degradation of EPS matrixes remains unclear.					
33113670	9	40	theme	low	1446:1448	arg1	compounds					1474:1482	more low molecular single carbon compounds	1441:1482	more low molecular single carbon compounds	1441:1482	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	9	41	contain	contained	1431:1439	arg1	EPSs					1416:1419	Tightly-bound EPSs	1402:1419	Tightly-bound EPSs (TB-EPSs)	1402:1429	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	9	41	contain	contained	1431:1439	arg2	compounds					1474:1482	more low molecular single carbon compounds	1441:1482	more low molecular single carbon compounds	1441:1482	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	9	41	contain	contained	1431:1439	arg1	TB-EPSs					1422:1428	TB-EPSs	1422:1428	TB-EPSs	1422:1428	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	9	42	theme	single	1460:1465	arg1	compounds					1474:1482	more low molecular single carbon compounds	1441:1482	more low molecular single carbon compounds	1441:1482	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	0	43	theme	polymeric	70:78	arg1	EPS					91:93	EPS	91:93	EPS	91:93	Characteristics and bacterial community dynamics during extracellular polymeric substance (EPS) degradation of cyanobacterial blooms.					
33113670	0	43	theme	polymeric	70:78	arg1	substance					80:88	polymeric substance	70:88	extracellular polymeric substance (EPS) degradation of cyanobacterial blooms	56:131	Characteristics and bacterial community dynamics during extracellular polymeric substance (EPS) degradation of cyanobacterial blooms.					
33113670	8	44	from	changes	1248:1254	arg1	EPSs					1299:1302	EPSs	1299:1302	EPSs	1299:1302	The changes of humic-like C1 and tryptophan-like C3 in EPSs were significantly correlated to the variations of microbial community composition and diversity.					
33113670	10	45	theme	organic	1681:1687	arg1	maters					1689:1694	organic maters	1681:1694	organic maters	1681:1694	Betaproteobacteria, Firmicute, Alphaproteobacteria, Sphingobacteria and Actinobacter were significantly correlated with the changes of organic maters through the humification process.					
33113670	8	46	theme	diversity	1391:1399	arg1	variations					1341:1350	the variations	1337:1350	the variations of microbial community composition and diversity	1337:1399	The changes of humic-like C1 and tryptophan-like C3 in EPSs were significantly correlated to the variations of microbial community composition and diversity.					
33113670	2	47	theme	coupling	353:360	arg1	phylogeny					320:328	the phylogeny	316:328	the phylogeny of microbial community coupling with the degradation of EPS	316:388	However, how the phylogeny of microbial community coupling with the degradation of EPS matrixes remains unclear.					
33113670	7	48	theme	hydrolytic	1122:1131	arg1	activities					1133:1142	different hydrolytic activities	1112:1142	different hydrolytic activities	1112:1142	Size-fractionated microorganisms exhibited different hydrolytic activities when interacting with macromolecules, but they did not present different phylogenetic compositions.					
33113670	8	49	theme	tryptophan-like	1277:1291	arg1	C3					1293:1294	tryptophan-like C3	1277:1294	tryptophan-like C3	1277:1294	The changes of humic-like C1 and tryptophan-like C3 in EPSs were significantly correlated to the variations of microbial community composition and diversity.					
33113670	10	50	theme	humification	1708:1719	arg1	process					1721:1727	the humification process	1704:1727	the humification process	1704:1727	Betaproteobacteria, Firmicute, Alphaproteobacteria, Sphingobacteria and Actinobacter were significantly correlated with the changes of organic maters through the humification process.					
33113670	4	51	theme	carbon	653:658	arg1	EPSs					629:632	fractionated EPSs	616:632	fractionated EPSs	616:632	In this study, fractionated EPSs were prepared as a carbon source to enrich different particle size microorganisms.					
33113670	4	51	theme	carbon	653:658	arg1	source					660:665	a carbon source	651:665	a carbon source to enrich different particle size microorganisms	651:714	In this study, fractionated EPSs were prepared as a carbon source to enrich different particle size microorganisms.					
33113670	7	52	theme	Size-fractionated	1069:1085	arg1	microorganisms					1087:1100	Size-fractionated microorganisms	1069:1100	Size-fractionated microorganisms	1069:1100	Size-fractionated microorganisms exhibited different hydrolytic activities when interacting with macromolecules, but they did not present different phylogenetic compositions.					
33113670	3	53	from	cycling	500:506	arg1	phycosphere					515:525	the phycosphere	511:525	the phycosphere	511:525	A better understanding of the dynamic process not only give insight into the carbon cycling in the phycosphere, but also provide a new approach for controlling the cyanobacteria bloom.					
33113670	3	54	theme	new	547:549	arg1	approach					551:558	a new approach	545:558	a new approach for controlling the cyanobacteria bloom	545:598	A better understanding of the dynamic process not only give insight into the carbon cycling in the phycosphere, but also provide a new approach for controlling the cyanobacteria bloom.					
33113670	9	55	theme	diverse	1522:1528	arg1	microorganisms					1530:1543	more diverse microorganisms	1517:1543	more diverse microorganisms	1517:1543	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	6	56	theme	components	959:968	arg1	change					941:946	the change	937:946	the change of organic components in the degradation process that causes the microbial community to follow a certain succession law	937:1066	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	6	56	theme	components	959:968	arg1	it					931:932	it	931:932	it	931:932	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	11	57	dep	Meanwhile	1730:1738	arg1	loosely-bound					1741:1753	loosely-bound	1741:1753	loosely-bound	1741:1753	Meanwhile, loosely-bound EPSs (LB-EPSs), which composed of more macromolecules, were more affiliated to a functional organized microbial community.					
33113670	4	58	theme	fractionated	616:627	arg1	EPSs					629:632	fractionated EPSs	616:632	fractionated EPSs	616:632	In this study, fractionated EPSs were prepared as a carbon source to enrich different particle size microorganisms.					
33113670	4	58	theme	fractionated	616:627	arg1	source					660:665	a carbon source	651:665	a carbon source to enrich different particle size microorganisms	651:714	In this study, fractionated EPSs were prepared as a carbon source to enrich different particle size microorganisms.					
33113670	5	59	theme	emission	860:867	arg1	EEM					877:879	emission matrix (EEM) and Illumina sequencing	860:904	EEM	877:879	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	8	60	theme	C1	1270:1271	arg1	changes					1248:1254	The changes	1244:1254	The changes of humic-like C1 and tryptophan-like C3 in EPSs	1244:1302	The changes of humic-like C1 and tryptophan-like C3 in EPSs were significantly correlated to the variations of microbial community composition and diversity.					
33113670	4	61	theme	size	696:699	arg1	microorganisms					701:714	different particle size microorganisms	677:714	different particle size microorganisms	677:714	In this study, fractionated EPSs were prepared as a carbon source to enrich different particle size microorganisms.					
33113670	5	62	from	communities	769:779	arg1	Changes					717:723	Changes	717:723	Changes of organic components in EPSs and microbial communities in the degradation process	717:806	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	1	63	theme	polymeric	148:156	arg1	EPSs					170:173	EPSs	170:173	EPSs	170:173	Extracellular polymeric substances (EPSs), which composed of different organic components, play an important role in the formation of mucilaginous cyanobacterial bloom.					
33113670	1	63	theme	polymeric	148:156	arg1	substances					158:167	Extracellular polymeric substances	134:167	Extracellular polymeric substances (EPSs)	134:174	Extracellular polymeric substances (EPSs), which composed of different organic components, play an important role in the formation of mucilaginous cyanobacterial bloom.					
33113670	13	64	theme	molecules	2059:2067	arg1	content					2037:2043	the content	2033:2043	the content of some small molecules	2033:2067	And the content of some small molecules was briefly increased during the degradation process.					
33113670	11	65	theme	microbial	1857:1865	arg1	community					1867:1875	a functional organized microbial community	1834:1875	a functional organized microbial community	1834:1875	Meanwhile, loosely-bound EPSs (LB-EPSs), which composed of more macromolecules, were more affiliated to a functional organized microbial community.					
33113670	1	66	theme	organic	205:211	arg1	components					213:222	different organic components	195:222	different organic components	195:222	Extracellular polymeric substances (EPSs), which composed of different organic components, play an important role in the formation of mucilaginous cyanobacterial bloom.					
33113670	5	67	theme	Illumina	886:893	arg1	sequencing					895:904	emission matrix (EEM) and Illumina sequencing	860:904	sequencing	895:904	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	6	68	theme	microbial	1013:1021	arg1	community					1023:1031	the microbial community	1009:1031	the microbial community to follow a certain succession law	1009:1066	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	8	69	theme	community	1365:1373	arg1	composition					1375:1385	microbial community composition	1355:1385	microbial community composition	1355:1385	The changes of humic-like C1 and tryptophan-like C3 in EPSs were significantly correlated to the variations of microbial community composition and diversity.					
33113670	11	70	theme	functional	1836:1845	arg1	community					1867:1875	a functional organized microbial community	1834:1875	a functional organized microbial community	1834:1875	Meanwhile, loosely-bound EPSs (LB-EPSs), which composed of more macromolecules, were more affiliated to a functional organized microbial community.					
33113670	5	71	theme	microbial	759:767	arg1	communities					769:779	microbial communities	759:779	microbial communities	759:779	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	4	72	theme	different	677:685	arg1	microorganisms					701:714	different particle size microorganisms	677:714	different particle size microorganisms	677:714	In this study, fractionated EPSs were prepared as a carbon source to enrich different particle size microorganisms.					
33113670	2	73	with	phylogeny	320:328	arg1	degradation					371:381	the degradation	367:381	the degradation of EPS	367:388	However, how the phylogeny of microbial community coupling with the degradation of EPS matrixes remains unclear.					
33113670	0	74	theme	blooms	126:131	arg1	degradation					96:106	extracellular polymeric substance (EPS) degradation	56:106	extracellular polymeric substance (EPS) degradation of cyanobacterial blooms	56:131	Characteristics and bacterial community dynamics during extracellular polymeric substance (EPS) degradation of cyanobacterial blooms.					
33113670	1	75	theme	important	233:241	arg1	role					243:246	an important role	230:246	an important role	230:246	Extracellular polymeric substances (EPSs), which composed of different organic components, play an important role in the formation of mucilaginous cyanobacterial bloom.					
33113670	3	76	theme	process	454:460	arg1	understanding					425:437	A better understanding	416:437	A better understanding of the dynamic process	416:460	A better understanding of the dynamic process not only give insight into the carbon cycling in the phycosphere, but also provide a new approach for controlling the cyanobacteria bloom.					
33113670	7	77	theme	different	1207:1215	arg1	compositions					1230:1241	different phylogenetic compositions	1207:1241	different phylogenetic compositions	1207:1241	Size-fractionated microorganisms exhibited different hydrolytic activities when interacting with macromolecules, but they did not present different phylogenetic compositions.					
33113670	14	78	theme	specialized	2229:2239	arg1	communities					2251:2261	specialized microbial communities	2229:2261	specialized microbial communities	2229:2261	Therefore, in order to prevent algal bloom from reducing cellular aggregation by decreasing viscous EPSs, specialized microbial communities should be considered in the phycosphere.					
33113670	2	79	theme	EPS	386:388	arg1	degradation					371:381	the degradation	367:381	the degradation of EPS	367:388	However, how the phylogeny of microbial community coupling with the degradation of EPS matrixes remains unclear.					
33113670	0	80	theme	community	30:38	arg1	dynamics					40:47	bacterial community dynamics	20:47	bacterial community dynamics	20:47	Characteristics and bacterial community dynamics during extracellular polymeric substance (EPS) degradation of cyanobacterial blooms.					
33113670	1	81	theme	bloom	296:300	arg1	formation					255:263	the formation	251:263	the formation of mucilaginous cyanobacterial bloom	251:300	Extracellular polymeric substances (EPSs), which composed of different organic components, play an important role in the formation of mucilaginous cyanobacterial bloom.					
33113670	6	82	theme	succession	1053:1062	arg1	law					1064:1066	a certain succession law	1043:1066	a certain succession law	1043:1066	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	5	83	theme	degradation	788:798	arg1	process					800:806	the degradation process	784:806	the degradation process	784:806	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	2	84	theme	community	343:351	arg1	coupling					353:360	microbial community coupling	333:360	microbial community coupling	333:360	However, how the phylogeny of microbial community coupling with the degradation of EPS matrixes remains unclear.					
33113670	12	85	theme	polysaccharide	1981:1994	arg1	structures					1996:2005	the polysaccharide structures	1977:2005	the polysaccharide structures	1977:2005	When Gammaproteobacteria and Betaproteobacteria were involved in LB-EPS degradation as indicators, the polysaccharide structures changed dramatically.					
33113670	5	86	theme	components	736:745	arg1	Changes					717:723	Changes	717:723	Changes of organic components in EPSs and microbial communities in the degradation process	717:806	Changes of organic components in EPSs and microbial communities in the degradation process were investigated using Fluorescence excitation and emission matrix (EEM) and Illumina sequencing.					
33113670	6	87	from	change	941:946	arg1	process					989:995	the degradation process	973:995	the degradation process	973:995	The results showed that it is the change of organic components in the degradation process that causes the microbial community to follow a certain succession law.					
33113670	11	88	theme	Meanwhile	1730:1738	arg1	LB-EPSs					1761:1767	LB-EPSs	1761:1767	LB-EPSs	1761:1767	Meanwhile, loosely-bound EPSs (LB-EPSs), which composed of more macromolecules, were more affiliated to a functional organized microbial community.					
33113670	11	88	theme	Meanwhile	1730:1738	arg1	EPSs					1755:1758	Meanwhile, loosely-bound EPSs	1730:1758	Meanwhile, loosely-bound EPSs (LB-EPSs)	1730:1768	Meanwhile, loosely-bound EPSs (LB-EPSs), which composed of more macromolecules, were more affiliated to a functional organized microbial community.					
33113670	9	89	theme	molecular	1450:1458	arg1	compounds					1474:1482	more low molecular single carbon compounds	1441:1482	more low molecular single carbon compounds	1441:1482	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33113670	9	90	theme	carbon	1467:1472	arg1	compounds					1474:1482	more low molecular single carbon compounds	1441:1482	more low molecular single carbon compounds	1441:1482	Tightly-bound EPSs (TB-EPSs) contained more low molecular single carbon compounds and were more easily utilized by more diverse microorganisms.					
33677040	0	0	theme	anti-proliferative	90:107	arg1	activities					109:118	anti-proliferative activities	90:118	anti-proliferative activities	90:118	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	3	1	theme	morphological	720:732	arg1	characteristics					734:748	irregular, smooth and compact morphological characteristics	690:748	irregular, smooth and compact morphological characteristics	690:748	JRP belonged to non-crystalline substance, with irregular, smooth and compact morphological characteristics.					
33677040	2	2	theme	molecular	579:587	arg1	weight					589:594	the molecular weight	575:594	the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da	575:639	Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da.					
33677040	1	3	theme	digestion	270:278	arg1	effects					232:238	the effects	228:238	the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP)	228:416	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	5	4	theme	apoptosis	1205:1213	arg1	induction					1192:1200	induction	1192:1200	induction of apoptosis	1192:1213	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	2	5	from	glucose	462:468	arg1	ratio					533:537	a molar ratio	525:537	a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3	525:567	Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da.					
33677040	5	6	dep	accumulation	1248:1259	arg1	the					1230:1232	the	1230:1232	the	1230:1232	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	3	7	theme	non-crystalline	658:672	arg1	substance					674:682	non-crystalline substance	658:682	non-crystalline substance	658:682	JRP belonged to non-crystalline substance, with irregular, smooth and compact morphological characteristics.					
33677040	5	8	theme	HeLa	1178:1181	arg1	cells					1183:1187	HeLa cells	1178:1187	HeLa cells	1178:1187	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	0	9	from	Effect	0:5	arg1	characteristics					70:84	structural characteristics	59:84	structural characteristics	59:84	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	0	9	from	Effect	0:5	arg1	activities					109:118	anti-proliferative activities	90:118	anti-proliferative activities	90:118	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	5	10	from	result	1220:1225	arg1	phase					1291:1295	G2/M phase	1286:1295	G2/M phase	1286:1295	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	6	11	contain	possessed	1453:1461	arg1	JRP					1409:1411	JRP	1409:1411	JRP processed by gastrointestinal digestion	1409:1451	These findings suggested that JRP processed by gastrointestinal digestion possessed more potential anti-proliferative applications that need to be exploited.					
33677040	6	11	contain	possessed	1453:1461	arg2	applications					1497:1508	more potential anti-proliferative applications	1463:1508	more potential anti-proliferative applications that need to be exploited	1463:1534	These findings suggested that JRP processed by gastrointestinal digestion possessed more potential anti-proliferative applications that need to be exploited.					
33677040	5	12	theme	activities	1081:1090	arg1	measurements					1046:1057	measurements	1046:1057	measurements of anti-proliferation activities	1046:1090	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	4	13	theme	sugar	1012:1016	arg1	increase					991:998	the increase	987:998	the increase of reducing sugar in digestive juice	987:1035	Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice.					
33677040	2	14	from	ribose	471:476	arg1	ratio					533:537	a molar ratio	525:537	a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3	525:567	Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da.					
33677040	1	15	from	activities	346:355	arg1	shells					385:390	the shells	381:390	the shells of Juglans regia L. (JRP)	381:416	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	5	16	theme	excessive	1234:1242	arg1	accumulation					1248:1259	excessive ROS accumulation	1234:1259	excessive ROS accumulation	1234:1259	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	5	17	theme	G2/M	1286:1289	arg1	phase					1291:1295	G2/M phase	1286:1295	G2/M phase	1286:1295	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	0	18	theme	polysaccharides	127:141	arg1	characteristics					70:84	structural characteristics	59:84	structural characteristics	59:84	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	0	18	theme	polysaccharides	127:141	arg1	activities					109:118	anti-proliferative activities	90:118	anti-proliferative activities	90:118	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	1	19	theme	regia	403:407	arg1	shells					385:390	the shells	381:390	the shells of Juglans regia L. (JRP)	381:416	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	1	20	from	characteristics	307:321	arg1	shells					385:390	the shells	381:390	the shells of Juglans regia L. (JRP)	381:416	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	4	21	theme	physicochemical	813:827	arg1	properties					829:838	the physicochemical properties	809:838	the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology	809:939	Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice.					
33677040	4	22	theme	digestive	1021:1029	arg1	juice					1031:1035	digestive juice	1021:1035	digestive juice	1021:1035	Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice.					
33677040	2	23	from	galactose	479:487	arg1	ratio					533:537	a molar ratio	525:537	a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3	525:567	Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da.					
33677040	1	24	from	shells	385:390	arg1	characteristics					307:321	structural characteristics	296:321	structural characteristics	296:321	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	1	24	from	shells	385:390	arg1	activities					346:355	anti-proliferative activities	327:355	anti-proliferative activities	327:355	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	1	24	from	shells	385:390	arg1	polysaccharides					360:374	polysaccharides	360:374	polysaccharides from the shells of Juglans regia L. (JRP)	360:416	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	1	25	theme	present	183:189	arg1	research					191:198	The present research	179:198	The present research	179:198	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	2	26	dep	4.55 × 105 Da	627:639	arg1	to					624:625	to	624:625	to	624:625	Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da.					
33677040	6	27	dep	potential	1468:1476	arg1	anti-proliferative					1478:1495	anti-proliferative	1478:1495	anti-proliferative	1478:1495	These findings suggested that JRP processed by gastrointestinal digestion possessed more potential anti-proliferative applications that need to be exploited.					
33677040	4	28	theme	gastrointestinal	772:787	arg1	digestion					789:797	gastrointestinal digestion	772:797	gastrointestinal digestion in vitro	772:806	Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice.					
33677040	5	29	theme	cells	1183:1187	arg1	result					1220:1225	a result	1218:1225	a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP	1218:1376	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	5	29	theme	cells	1183:1187	arg1	viabilities					1163:1173	the viabilities	1159:1173	the viabilities of HeLa cells	1159:1187	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	3	30	theme	smooth	701:706	arg1	characteristics					734:748	irregular, smooth and compact morphological characteristics	690:748	irregular, smooth and compact morphological characteristics	690:748	JRP belonged to non-crystalline substance, with irregular, smooth and compact morphological characteristics.					
33677040	5	31	theme	ROS	1244:1246	arg1	accumulation					1248:1259	excessive ROS accumulation	1234:1259	excessive ROS accumulation	1234:1259	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	4	32	theme	crystalline	903:913	arg1	properties					915:924	crystalline properties	903:924	crystalline properties	903:924	Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice.					
33677040	3	33	theme	irregular	690:698	arg1	characteristics					734:748	irregular, smooth and compact morphological characteristics	690:748	irregular, smooth and compact morphological characteristics	690:748	JRP belonged to non-crystalline substance, with irregular, smooth and compact morphological characteristics.					
33677040	1	34	theme	structural	296:305	arg1	characteristics					307:321	structural characteristics	296:321	structural characteristics	296:321	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	2	35	theme	10.7:4.9:16.4:2.3:10.8:2.3	542:567	arg1	ratio					533:537	a molar ratio	525:537	a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3	525:567	Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da.					
33677040	5	36	theme	digested	1121:1128	arg1	JRP					1130:1132	the digested JRP	1117:1132	the digested JRP	1117:1132	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	5	37	theme	undigested	1363:1372	arg1	JRP					1374:1376	undigested JRP	1363:1376	undigested JRP	1363:1376	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	3	38	theme	compact	712:718	arg1	characteristics					734:748	irregular, smooth and compact morphological characteristics	690:748	irregular, smooth and compact morphological characteristics	690:748	JRP belonged to non-crystalline substance, with irregular, smooth and compact morphological characteristics.					
33677040	0	39	theme	in	10:11	arg1	digestion					46:54	in vitro simulated gastrointestinal digestion	10:54	in vitro simulated gastrointestinal digestion	10:54	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	2	40	from	mannose	490:496	arg1	ratio					533:537	a molar ratio	525:537	a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3	525:567	Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da.					
33677040	0	41	theme	gastrointestinal	29:44	arg1	digestion					46:54	in vitro simulated gastrointestinal digestion	10:54	in vitro simulated gastrointestinal digestion	10:54	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	5	42	theme	cell	1265:1268	arg1	arrest					1276:1281	cell cycle arrest	1265:1281	cell cycle arrest	1265:1281	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	4	43	theme	reducing	1003:1010	arg1	sugar					1012:1016	reducing sugar	1003:1016	reducing sugar	1003:1016	Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice.					
33677040	5	44	theme	arrest	1276:1281	arg1	result					1220:1225	a result	1218:1225	a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP	1218:1376	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	5	44	theme	arrest	1276:1281	arg1	viabilities					1163:1173	the viabilities	1159:1173	the viabilities of HeLa cells	1159:1187	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	0	45	theme	simulated	19:27	arg1	digestion					46:54	in vitro simulated gastrointestinal digestion	10:54	in vitro simulated gastrointestinal digestion	10:54	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	1	46	theme	anti-proliferative	327:344	arg1	activities					346:355	anti-proliferative activities	327:355	anti-proliferative activities	327:355	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	2	47	theme	molar	527:531	arg1	ratio					533:537	a molar ratio	525:537	a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3	525:567	Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da.					
33677040	0	48	from	characteristics	70:84	arg1	shells					152:157	the shells	148:157	the shells of Juglans regia L	148:176	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	4	49	theme	molecular	857:865	arg1	weight					867:872	molecular weight	857:872	molecular weight	857:872	Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice.					
33677040	1	50	dep	regia	403:407	arg1	L					409:409	L	409:409	Juglans regia L. (JRP)	395:416	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	6	51	theme	gastrointestinal	1426:1441	arg1	digestion					1443:1451	gastrointestinal digestion	1426:1451	gastrointestinal digestion	1426:1451	These findings suggested that JRP processed by gastrointestinal digestion possessed more potential anti-proliferative applications that need to be exploited.					
33677040	0	52	theme	regia	170:174	arg1	L					176:176	Juglans regia L	162:176	Juglans regia L	162:176	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	5	53	theme	accumulation	1248:1259	arg1	result					1220:1225	a result	1218:1225	a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP	1218:1376	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	5	53	theme	accumulation	1248:1259	arg1	viabilities					1163:1173	the viabilities	1159:1173	the viabilities of HeLa cells	1159:1187	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	4	54	theme	monosaccharide	875:888	arg1	composition					890:900	monosaccharide composition	875:900	monosaccharide composition	875:900	Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice.					
33677040	5	55	from	phase	1291:1295	arg1	accumulation					1248:1259	excessive ROS accumulation	1234:1259	excessive ROS accumulation	1234:1259	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	5	55	from	phase	1291:1295	arg1	result					1220:1225	a result	1218:1225	a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP	1218:1376	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	5	55	from	phase	1291:1295	arg1	viabilities					1163:1173	the viabilities	1159:1173	the viabilities of HeLa cells	1159:1187	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	5	55	from	phase	1291:1295	arg1	arrest					1276:1281	cell cycle arrest	1265:1281	cell cycle arrest	1265:1281	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	0	56	theme	digestion	46:54	arg1	Effect					0:5	Effect	0:5	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.	0:177	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	0	57	theme	Juglans	162:168	arg1	L					176:176	Juglans regia L	162:176	Juglans regia L	162:176	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	2	58	from	rhamnose	513:520	arg1	ratio					533:537	a molar ratio	525:537	a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3	525:567	Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da.					
33677040	2	59	from	arabinose	499:507	arg1	ratio					533:537	a molar ratio	525:537	a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3	525:567	Results suggested that JRP was composed of glucose, ribose, galactose, mannose, arabinose and rhamnose in a molar ratio of 10.7:4.9:16.4:2.3:10.8:2.3, with the molecular weight distributed from 3.21 × 105 to 4.55 × 105 Da.					
33677040	4	60	from	increase	991:998	arg1	juice					1031:1035	digestive juice	1021:1035	digestive juice	1021:1035	Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice.					
33677040	1	61	theme	polysaccharides	360:374	arg1	characteristics					307:321	structural characteristics	296:321	structural characteristics	296:321	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	1	61	theme	polysaccharides	360:374	arg1	activities					346:355	anti-proliferative activities	327:355	anti-proliferative activities	327:355	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	1	62	from	effects	232:238	arg1	characteristics					307:321	structural characteristics	296:321	structural characteristics	296:321	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	1	62	from	effects	232:238	arg1	activities					346:355	anti-proliferative activities	327:355	anti-proliferative activities	327:355	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	5	63	theme	anti-proliferation	1062:1079	arg1	activities					1081:1090	anti-proliferation activities	1062:1090	anti-proliferation activities	1062:1090	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	0	64	theme	L	176:176	arg1	shells					152:157	the shells	148:157	the shells of Juglans regia L	148:176	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	0	65	dep	in	10:11	arg1	vitro					13:17	vitro	13:17	vitro	13:17	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	5	66	theme	cycle	1270:1274	arg1	arrest					1276:1281	cell cycle arrest	1265:1281	cell cycle arrest	1265:1281	Through measurements of anti-proliferation activities, the results showed that the digested JRP could remarkably inhibit the viabilities of HeLa cells by induction of apoptosis as a result of the excessive ROS accumulation and cell cycle arrest at G2/M phase, all of which were pronouncedly stronger than the ones induced by undigested JRP.					
33677040	0	67	from	shells	152:157	arg1	characteristics					70:84	structural characteristics	59:84	structural characteristics	59:84	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	0	67	from	shells	152:157	arg1	activities					109:118	anti-proliferative activities	90:118	anti-proliferative activities	90:118	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	0	67	from	shells	152:157	arg1	polysaccharides					127:141	the polysaccharides	123:141	the polysaccharides from the shells of Juglans regia L	123:176	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	6	68	theme	potential	1468:1476	arg1	applications					1497:1508	more potential anti-proliferative applications	1463:1508	more potential anti-proliferative applications that need to be exploited	1463:1534	These findings suggested that JRP processed by gastrointestinal digestion possessed more potential anti-proliferative applications that need to be exploited.					
33677040	0	69	from	activities	109:118	arg1	shells					152:157	the shells	148:157	the shells of Juglans regia L	148:176	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	1	70	theme	simulated	243:251	arg1	digestion					270:278	simulated gastrointestinal digestion	243:278	simulated gastrointestinal digestion in vitro	243:287	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	0	71	theme	structural	59:68	arg1	characteristics					70:84	structural characteristics	59:84	structural characteristics	59:84	Effect of in vitro simulated gastrointestinal digestion on structural characteristics and anti-proliferative activities of the polysaccharides from the shells of Juglans regia L.					
33677040	1	72	dep	characteristics	307:321	arg1	the					292:294	the	292:294	the	292:294	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
33677040	4	73	theme	JRP	843:845	arg1	properties					829:838	the physicochemical properties	809:838	the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology	809:939	Nevertheless, during gastrointestinal digestion in vitro, the physicochemical properties of JRP including molecular weight, monosaccharide composition, crystalline properties and morphology were significantly changed, accompanying with the increase of reducing sugar in digestive juice.					
33677040	1	74	theme	gastrointestinal	253:268	arg1	digestion					270:278	simulated gastrointestinal digestion	243:278	simulated gastrointestinal digestion in vitro	243:287	The present research was designed to investigate the effects of simulated gastrointestinal digestion in vitro on the structural characteristics and anti-proliferative activities of polysaccharides from the shells of Juglans regia L. (JRP).					
34229017	4	0	theme	denser	701:706	arg1	structure					708:716	denser structure	701:716	denser structure	701:716	The films presented a smoother surface and denser structure than PAX.					
34229017	6	1	theme	food	1005:1008	arg1	freshness					1010:1018	protein-rich food freshness	992:1018	protein-rich food freshness	992:1018	Therefore, it has the potential to become a pH-sensitive film used in monitoring protein-rich food freshness.					
34229017	0	2	theme	pomace	91:96	arg1	extract					98:104	raspberry pomace extract	81:104	raspberry pomace extract for monitoring protein-rich food freshness	81:147	Colorimetric films based on pectin/sodium alginate/xanthan gum incorporated with raspberry pomace extract for monitoring protein-rich food freshness.					
34229017	5	3	from	pH 1-13	782:788	arg1	discoloration					765:777	an excellent discoloration	752:777	an excellent discoloration at pH 1-13	752:788	Furthermore, PAXR15 had an excellent discoloration at pH 1-13, especially at pH 5-10, the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes.					
34229017	1	4	theme	pectin/sodium	264:276	arg1	PAX					315:317	PAX	315:317	PAX	315:317	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	4	theme	pectin/sodium	264:276	arg1	film					309:312	pectin/sodium alginate/xanthan gum composite film	264:312	pectin/sodium alginate/xanthan gum composite film (PAX)	264:318	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	5	with	extracts	167:174	arg1	concentrations					197:210	different concentrations	187:210	different concentrations (0.5 g/L, 1.5 g/L and 3 g/L)	187:239	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	5	with	extracts	167:174	arg1	3 g/L					234:238	3 g/L	234:238	3 g/L	234:238	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	5	with	extracts	167:174	arg1	1.5 g/L					222:228	1.5 g/L	222:228	1.5 g/L	222:228	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	5	with	extracts	167:174	arg1	0.5 g/L					213:219	0.5 g/L	213:219	0.5 g/L	213:219	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	3	6	theme	lower	542:546	arg1	ratio					592:596	lower water vapor permeability and water swelling ratio	542:596	lower water vapor permeability and water swelling ratio	542:596	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	1	7	theme	alginate/xanthan	278:293	arg1	PAX					315:317	PAX	315:317	PAX	315:317	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	7	theme	alginate/xanthan	278:293	arg1	film					309:312	pectin/sodium alginate/xanthan gum composite film	264:312	pectin/sodium alginate/xanthan gum composite film (PAX)	264:318	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	5	8	contain	had	748:750	arg1	PAXR15					741:746	PAXR15	741:746	PAXR15	741:746	Furthermore, PAXR15 had an excellent discoloration at pH 1-13, especially at pH 5-10, the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes.					
34229017	5	8	contain	had	748:750	arg2	changes					824:830	the color changes	814:830	the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes	814:908	Furthermore, PAXR15 had an excellent discoloration at pH 1-13, especially at pH 5-10, the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes.					
34229017	5	8	contain	had	748:750	arg2	discoloration					765:777	an excellent discoloration	752:777	an excellent discoloration at pH 1-13	752:788	Furthermore, PAXR15 had an excellent discoloration at pH 1-13, especially at pH 5-10, the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes.					
34229017	3	9	theme	water	548:552	arg1	permeability					560:571	water vapor permeability	548:571	water vapor permeability	548:571	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	1	10	theme	gum	295:297	arg1	PAX					315:317	PAX	315:317	PAX	315:317	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	10	theme	gum	295:297	arg1	film					309:312	pectin/sodium alginate/xanthan gum composite film	264:312	pectin/sodium alginate/xanthan gum composite film (PAX)	264:318	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	0	11	theme	food	134:137	arg1	freshness					139:147	protein-rich food freshness	121:147	protein-rich food freshness	121:147	Colorimetric films based on pectin/sodium alginate/xanthan gum incorporated with raspberry pomace extract for monitoring protein-rich food freshness.					
34229017	0	12	theme	protein-rich	121:132	arg1	freshness					139:147	protein-rich food freshness	121:147	protein-rich food freshness	121:147	Colorimetric films based on pectin/sodium alginate/xanthan gum incorporated with raspberry pomace extract for monitoring protein-rich food freshness.					
34229017	1	13	theme	different	187:195	arg1	concentrations					197:210	different concentrations	187:210	different concentrations (0.5 g/L, 1.5 g/L and 3 g/L)	187:239	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	13	theme	different	187:195	arg1	3 g/L					234:238	3 g/L	234:238	3 g/L	234:238	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	13	theme	different	187:195	arg1	1.5 g/L					222:228	1.5 g/L	222:228	1.5 g/L	222:228	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	13	theme	different	187:195	arg1	0.5 g/L					213:219	0.5 g/L	213:219	0.5 g/L	213:219	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	0	14	theme	Colorimetric	0:11	arg1	films					13:17	Colorimetric films	0:17	Colorimetric films based on pectin/sodium alginate/xanthan gum	0:61	Colorimetric films based on pectin/sodium alginate/xanthan gum incorporated with raspberry pomace extract for monitoring protein-rich food freshness.					
34229017	1	15	theme	composite	299:307	arg1	PAX					315:317	PAX	315:317	PAX	315:317	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	15	theme	composite	299:307	arg1	film					309:312	pectin/sodium alginate/xanthan gum composite film	264:312	pectin/sodium alginate/xanthan gum composite film (PAX)	264:318	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	2	16	dep	showed	457:462	arg1	had					468:470	had	468:470	showed RPE had good compatibility with PAX	457:498	Fourier Transform Infrared and Scanning Electron Microscopy analysis showed RPE had good compatibility with PAX.					
34229017	3	17	theme	water	577:581	arg1	swelling					583:590	water swelling	577:590	water swelling	577:590	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	6	18	contain	has	925:927	arg1	it					922:923	it	922:923	it	922:923	Therefore, it has the potential to become a pH-sensitive film used in monitoring protein-rich food freshness.					
34229017	6	18	contain	has	925:927	arg2	potential					933:941	the potential to become a pH-sensitive film used in monitoring protein-rich food freshness	929:1018	the potential to become a pH-sensitive film used in monitoring protein-rich food freshness	929:1018	Therefore, it has the potential to become a pH-sensitive film used in monitoring protein-rich food freshness.					
34229017	3	19	theme	vapor	554:558	arg1	permeability					560:571	water vapor permeability	548:571	water vapor permeability	548:571	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	2	20	theme	Electron	428:435	arg1	Microscopy					437:446	Scanning Electron Microscopy	419:446	Scanning Electron Microscopy analysis	419:455	Fourier Transform Infrared and Scanning Electron Microscopy analysis showed RPE had good compatibility with PAX.					
34229017	2	21	theme	Microscopy	437:446	arg1	analysis					448:455	Scanning Electron Microscopy analysis	419:455	Scanning Electron Microscopy analysis	419:455	Fourier Transform Infrared and Scanning Electron Microscopy analysis showed RPE had good compatibility with PAX.					
34229017	6	22	theme	pH-sensitive	955:966	arg1	film					968:971	a pH-sensitive film	953:971	a pH-sensitive film used in monitoring protein-rich food freshness	953:1018	Therefore, it has the potential to become a pH-sensitive film used in monitoring protein-rich food freshness.					
34229017	3	23	theme	permeability	560:571	arg1	ratio					592:596	lower water vapor permeability and water swelling ratio	542:596	lower water vapor permeability and water swelling ratio	542:596	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	2	24	theme	Scanning	419:426	arg1	Microscopy					437:446	Scanning Electron Microscopy	419:446	Scanning Electron Microscopy analysis	419:455	Fourier Transform Infrared and Scanning Electron Microscopy analysis showed RPE had good compatibility with PAX.					
34229017	0	25	theme	alginate/xanthan	42:57	arg1	gum					59:61	pectin/sodium alginate/xanthan gum	28:61	pectin/sodium alginate/xanthan gum	28:61	Colorimetric films based on pectin/sodium alginate/xanthan gum incorporated with raspberry pomace extract for monitoring protein-rich food freshness.					
34229017	1	26	dep	films	354:358	arg1	PAXR15					368:373	PAXR15	368:373	PAXR15	368:373	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	26	dep	films	354:358	arg1	PAXR5					361:365	PAXR5	361:365	PAXR5	361:365	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	26	dep	films	354:358	arg1	films					354:358	colorimetric raspberry films	331:358	colorimetric raspberry films (PAXR5, PAXR15 and PAXR30)	331:385	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	26	dep	films	354:358	arg1	PAXR30					379:384	PAXR30	379:384	PAXR30	379:384	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	27	dep	concentrations	197:210	arg1	concentrations					197:210	different concentrations	187:210	different concentrations (0.5 g/L, 1.5 g/L and 3 g/L)	187:239	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	27	dep	concentrations	197:210	arg1	3 g/L					234:238	3 g/L	234:238	3 g/L	234:238	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	27	dep	concentrations	197:210	arg1	1.5 g/L					222:228	1.5 g/L	222:228	1.5 g/L	222:228	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	27	dep	concentrations	197:210	arg1	0.5 g/L					213:219	0.5 g/L	213:219	0.5 g/L	213:219	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	5	28	theme	PAXR15	835:840	arg1	changes					824:830	the color changes	814:830	the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes	814:908	Furthermore, PAXR15 had an excellent discoloration at pH 1-13, especially at pH 5-10, the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes.					
34229017	0	29	theme	pectin/sodium	28:40	arg1	gum					59:61	pectin/sodium alginate/xanthan gum	28:61	pectin/sodium alginate/xanthan gum	28:61	Colorimetric films based on pectin/sodium alginate/xanthan gum incorporated with raspberry pomace extract for monitoring protein-rich food freshness.					
34229017	3	30	contain	had	538:540	arg2	opacity					624:630	opacity	624:630	opacity	624:630	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	3	30	contain	had	538:540	arg1	films					532:536	the raspberry films	518:536	the raspberry films	518:536	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	3	30	contain	had	538:540	arg2	strength					614:621	higher tensile strength	599:621	higher tensile strength	599:621	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	3	30	contain	had	538:540	arg2	ratio					592:596	lower water vapor permeability and water swelling ratio	542:596	lower water vapor permeability and water swelling ratio	542:596	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	3	30	contain	had	538:540	arg2	capacity					648:655	antioxidant capacity	636:655	antioxidant capacity	636:655	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	6	31	theme	protein-rich	992:1003	arg1	freshness					1010:1018	protein-rich food freshness	992:1018	protein-rich food freshness	992:1018	Therefore, it has the potential to become a pH-sensitive film used in monitoring protein-rich food freshness.					
34229017	3	32	theme	higher	599:604	arg1	strength					614:621	higher tensile strength	599:621	higher tensile strength	599:621	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	2	33	theme	good	472:475	arg1	compatibility					477:489	good compatibility	472:489	good compatibility	472:489	Fourier Transform Infrared and Scanning Electron Microscopy analysis showed RPE had good compatibility with PAX.					
34229017	3	34	theme	antioxidant	636:646	arg1	capacity					648:655	antioxidant capacity	636:655	antioxidant capacity	636:655	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	3	35	theme	swelling	583:590	arg1	ratio					592:596	lower water vapor permeability and water swelling ratio	542:596	lower water vapor permeability and water swelling ratio	542:596	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	1	36	theme	colorimetric	331:342	arg1	PAXR15					368:373	PAXR15	368:373	PAXR15	368:373	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	36	theme	colorimetric	331:342	arg1	PAXR5					361:365	PAXR5	361:365	PAXR5	361:365	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	36	theme	colorimetric	331:342	arg1	films					354:358	colorimetric raspberry films	331:358	colorimetric raspberry films (PAXR5, PAXR15 and PAXR30)	331:385	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	36	theme	colorimetric	331:342	arg1	PAXR30					379:384	PAXR30	379:384	PAXR30	379:384	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	4	37	theme	smoother	680:687	arg1	surface					689:695	a smoother surface	678:695	a smoother surface	678:695	The films presented a smoother surface and denser structure than PAX.					
34229017	1	38	theme	Raspberry	150:158	arg1	RPE					177:179	RPE	177:179	RPE	177:179	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	38	theme	Raspberry	150:158	arg1	extracts					167:174	Raspberry pomace extracts	150:174	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L)	150:239	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	39	theme	pomace	160:165	arg1	RPE					177:179	RPE	177:179	RPE	177:179	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	39	theme	pomace	160:165	arg1	extracts					167:174	Raspberry pomace extracts	150:174	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L)	150:239	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	0	40	theme	raspberry	81:89	arg1	extract					98:104	raspberry pomace extract	81:104	raspberry pomace extract for monitoring protein-rich food freshness	81:147	Colorimetric films based on pectin/sodium alginate/xanthan gum incorporated with raspberry pomace extract for monitoring protein-rich food freshness.					
34229017	3	41	theme	tensile	606:612	arg1	strength					614:621	higher tensile strength	599:621	higher tensile strength	599:621	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
34229017	5	42	theme	naked	899:903	arg1	eyes					905:908	the naked eyes	895:908	the naked eyes	895:908	Furthermore, PAXR15 had an excellent discoloration at pH 1-13, especially at pH 5-10, the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes.					
34229017	5	43	theme	excellent	755:763	arg1	discoloration					765:777	an excellent discoloration	752:777	an excellent discoloration at pH 1-13	752:788	Furthermore, PAXR15 had an excellent discoloration at pH 1-13, especially at pH 5-10, the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes.					
34229017	1	44	theme	raspberry	344:352	arg1	PAXR15					368:373	PAXR15	368:373	PAXR15	368:373	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	44	theme	raspberry	344:352	arg1	PAXR5					361:365	PAXR5	361:365	PAXR5	361:365	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	44	theme	raspberry	344:352	arg1	films					354:358	colorimetric raspberry films	331:358	colorimetric raspberry films (PAXR5, PAXR15 and PAXR30)	331:385	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	1	44	theme	raspberry	344:352	arg1	PAXR30					379:384	PAXR30	379:384	PAXR30	379:384	Raspberry pomace extracts (RPE) with different concentrations (0.5 g/L, 1.5 g/L and 3 g/L) were incorporated into pectin/sodium alginate/xanthan gum composite film (PAX) to prepare colorimetric raspberry films (PAXR5, PAXR15 and PAXR30).					
34229017	5	45	theme	color	818:822	arg1	changes					824:830	the color changes	814:830	the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes	814:908	Furthermore, PAXR15 had an excellent discoloration at pH 1-13, especially at pH 5-10, the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes.					
34229017	5	46	from	pink-red-brown-blue-dark	847:870	arg1	changes					824:830	the color changes	814:830	the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes	814:908	Furthermore, PAXR15 had an excellent discoloration at pH 1-13, especially at pH 5-10, the color changes of PAXR15 from pink-red-brown-blue-dark green distinguished by the naked eyes.					
34229017	3	47	theme	raspberry	522:530	arg1	films					532:536	the raspberry films	518:536	the raspberry films	518:536	Compared to PAX, the raspberry films had lower water vapor permeability and water swelling ratio, higher tensile strength, opacity and antioxidant capacity.					
33802984	3	0	with	blends	532:537	arg1	κ-carrageenan					544:556	κ-carrageenan	544:556	κ-carrageenan	544:556	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	3	0	with	blends	532:537	arg1	sulfate					574:580	chondroitin sulfate	562:580	chondroitin sulfate	562:580	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	1	1	theme	natural	171:177	arg1	polymers					179:186	synthetic and natural polymers	157:186	synthetic and natural polymers	157:186	Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering.					
33802984	4	2	from	impact	618:623	arg1	properties					779:788	porosity properties	770:788	porosity properties	770:788	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	2	from	impact	618:623	arg1	morphology					700:709	their morphology	694:709	their morphology	694:709	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	2	from	impact	618:623	arg1	uptake					759:764	their mechanical, thermal, water uptake	726:764	their mechanical, thermal, water uptake	726:764	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	3	theme	mesenchymal	975:985	arg1	cells					992:996	seeded human adipose-derived mesenchymal stem cells	946:996	seeded human adipose-derived mesenchymal stem cells	946:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	1	4	theme	polymers	179:186	arg1	composites					143:152	Hybrid composites	136:152	Hybrid composites of synthetic and natural polymers	136:186	Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering.					
33802984	1	4	theme	polymers	179:186	arg1	polymers					179:186	synthetic and natural polymers	157:186	synthetic and natural polymers	157:186	Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering.					
33802984	0	5	theme	Tissue	103:108	arg1	Applications					122:133	Bone Tissue Engineering Applications	98:133	Bone Tissue Engineering Applications	98:133	The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications.					
33802984	4	6	theme	osteoinductive	818:831	arg1	potential					833:841	their osteoinductive potential	812:841	their osteoinductive potential	812:841	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	7	theme	differentiation	927:941	arg1	evaluation					872:881	the evaluation	868:881	the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells	868:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	2	8	theme	attracting	311:320	arg1	interest					328:335	attracting great interest	311:335	attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties	311:439	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	2	9	theme	great	322:326	arg1	interest					328:335	attracting great interest	311:335	attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties	311:439	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	4	10	theme	preparation	632:642	arg1	methodology					644:654	the preparation methodology	628:654	the preparation methodology	628:654	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	0	11	theme	Bone	98:101	arg1	Applications					122:133	Bone Tissue Engineering Applications	98:133	Bone Tissue Engineering Applications	98:133	The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications.					
33802984	4	12	theme	osteogenic	916:925	arg1	differentiation					927:941	osteogenic differentiation	916:941	osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells	916:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	13	dep	mechanical	732:741	arg1	thermal					744:750	thermal	744:750	thermal	744:750	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	2	14	theme	due	386:388	arg1	scaffolds					376:384	novel biomedical scaffolds	359:384	novel biomedical scaffolds due to recent reports on its osteoinductive properties	359:439	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	4	15	theme	mechanical	732:741	arg1	uptake					759:764	their mechanical, thermal, water uptake	726:764	their mechanical, thermal, water uptake	726:764	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	16	theme	adhesion	891:898	arg1	evaluation					872:881	the evaluation	868:881	the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells	868:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	17	theme	composition	679:689	arg1	impact					618:623	The impact	614:623	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties	614:788	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	3	18	theme	chondroitin	562:572	arg1	sulfate					574:580	chondroitin sulfate	562:580	chondroitin sulfate	562:580	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	2	19	theme	osteoinductive	415:428	arg1	properties					430:439	its osteoinductive properties	411:439	its osteoinductive properties	411:439	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	0	20	theme	Engineering	110:120	arg1	Applications					122:133	Bone Tissue Engineering Applications	98:133	Bone Tissue Engineering Applications	98:133	The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications.					
33802984	4	21	theme	cell	886:889	arg1	adhesion					891:898	cell adhesion	886:898	cell adhesion	886:898	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	5	22	theme	ulvan	1050:1054	arg1	ability					1039:1045	the osteoinductive ability	1020:1045	the osteoinductive ability of ulvan	1020:1054	The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.					
33802984	3	23	theme	hybrid	462:467	arg1	scaffolds					486:494	hybrid polycaprolactone scaffolds	462:494	hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate	462:580	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	0	24	theme	Marine	4:9	arg1	Ulvan					26:30	The Marine Polysaccharide Ulvan	0:30	The Marine Polysaccharide Ulvan	0:30	The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications.					
33802984	4	25	theme	viability	901:909	arg1	evaluation					872:881	the evaluation	868:881	the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells	868:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	26	theme	porosity	770:777	arg1	properties					779:788	porosity properties	770:788	porosity properties	770:788	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	5	27	from	potential	1193:1201	arg1	development					1210:1220	the development	1206:1220	the development of biomedical scaffolds	1206:1244	The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.					
33802984	5	28	theme	tissue	1255:1260	arg1	regeneration					1262:1273	bone tissue regeneration	1250:1273	bone tissue regeneration applications	1250:1286	The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.					
33802984	1	29	theme	choice	211:216	arg1	materials					198:206	materials	198:206	materials of choice for bone tissue engineering	198:244	Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering.					
33802984	5	30	theme	regeneration	1262:1273	arg1	applications					1275:1286	bone tissue regeneration applications	1250:1286	bone tissue regeneration applications	1250:1286	The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.					
33802984	4	31	theme	stem	987:990	arg1	cells					992:996	seeded human adipose-derived mesenchymal stem cells	946:996	seeded human adipose-derived mesenchymal stem cells	946:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	5	32	theme	osteoinductive	1024:1037	arg1	ability					1039:1045	the osteoinductive ability	1020:1045	the osteoinductive ability of ulvan	1020:1054	The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.					
33802984	0	33	theme	Polysaccharide	11:24	arg1	Ulvan					26:30	The Marine Polysaccharide Ulvan	0:30	The Marine Polysaccharide Ulvan	0:30	The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications.					
33802984	2	34	theme	recent	393:398	arg1	reports					400:406	recent reports	393:406	recent reports on its osteoinductive properties	393:439	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	2	35	theme	scaffolds	376:384	arg1	development					344:354	the development	340:354	the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties	340:439	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	1	36	theme	bone	222:225	arg1	engineering					234:244	bone tissue engineering	222:244	bone tissue engineering	222:244	Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering.					
33802984	0	37	theme	Potent	40:45	arg1	Capacity					62:69	Potent Osteoinductive Capacity	40:69	Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications	40:133	The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications.					
33802984	2	38	theme	novel	359:363	arg1	scaffolds					376:384	novel biomedical scaffolds	359:384	novel biomedical scaffolds due to recent reports on its osteoinductive properties	359:439	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	3	39	theme	polycaprolactone	469:484	arg1	scaffolds					486:494	hybrid polycaprolactone scaffolds	462:494	hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate	462:580	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	1	40	theme	tissue	227:232	arg1	engineering					234:244	bone tissue engineering	222:244	bone tissue engineering	222:244	Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering.					
33802984	5	41	theme	biomedical	1225:1234	arg1	scaffolds					1236:1244	biomedical scaffolds	1225:1244	biomedical scaffolds	1225:1244	The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.					
33802984	4	42	theme	cells	992:996	arg1	viability					901:909	viability	901:909	viability	901:909	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	42	theme	cells	992:996	arg1	adhesion					891:898	cell adhesion	886:898	cell adhesion	886:898	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	42	theme	cells	992:996	arg1	differentiation					927:941	osteogenic differentiation	916:941	osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells	916:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	3	43	theme	scaffolds	486:494	arg1	series					452:457	a series	450:457	a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate	450:580	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	4	44	link	adipose-derived	959:973	arg1	cells					992:996	seeded human adipose-derived mesenchymal stem cells	946:996	seeded human adipose-derived mesenchymal stem cells	946:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	45	theme	seeded	946:951	arg1	cells					992:996	seeded human adipose-derived mesenchymal stem cells	946:996	seeded human adipose-derived mesenchymal stem cells	946:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	3	46	contain	containing	496:505	arg2	ulvan					507:511	ulvan	507:511	ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate	507:580	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	3	46	contain	containing	496:505	arg1	scaffolds					486:494	hybrid polycaprolactone scaffolds	462:494	hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate	462:580	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	2	47	from	reports	400:406	arg1	properties					430:439	its osteoinductive properties	411:439	its osteoinductive properties	411:439	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	2	48	theme	biomedical	365:374	arg1	scaffolds					376:384	novel biomedical scaffolds	359:384	novel biomedical scaffolds due to recent reports on its osteoinductive properties	359:439	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	0	49	theme	Osteoinductive	47:60	arg1	Capacity					62:69	Potent Osteoinductive Capacity	40:69	Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications	40:133	The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications.					
33802984	3	50	dep	either	513:518	arg1	alone					520:524	alone	520:524	alone	520:524	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	5	51	theme	polycaprolactone	1097:1112	arg1	matrix					1114:1119	the polycaprolactone matrix	1093:1119	the polycaprolactone matrix	1093:1119	The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.					
33802984	1	52	theme	Hybrid	136:141	arg1	composites					143:152	Hybrid composites	136:152	Hybrid composites of synthetic and natural polymers	136:186	Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering.					
33802984	1	52	theme	Hybrid	136:141	arg1	polymers					179:186	synthetic and natural polymers	157:186	synthetic and natural polymers	157:186	Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering.					
33802984	5	53	theme	bone	1250:1253	arg1	regeneration					1262:1273	bone tissue regeneration	1250:1273	bone tissue regeneration applications	1250:1286	The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.					
33802984	4	54	theme	adipose-derived	959:973	arg1	cells					992:996	seeded human adipose-derived mesenchymal stem cells	946:996	seeded human adipose-derived mesenchymal stem cells	946:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	2	55	theme	sulfated	283:290	arg1	polysaccharide					292:305	a biologically active marine sulfated polysaccharide	254:305	a biologically active marine sulfated polysaccharide	254:305	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	2	55	theme	sulfated	283:290	arg1	Ulvan					247:251	Ulvan	247:251	Ulvan	247:251	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	4	56	theme	human	953:957	arg1	cells					992:996	seeded human adipose-derived mesenchymal stem cells	946:996	seeded human adipose-derived mesenchymal stem cells	946:996	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	2	57	theme	marine	276:281	arg1	polysaccharide					292:305	a biologically active marine sulfated polysaccharide	254:305	a biologically active marine sulfated polysaccharide	254:305	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	2	57	theme	marine	276:281	arg1	Ulvan					247:251	Ulvan	247:251	Ulvan	247:251	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	4	58	theme	water	753:757	arg1	uptake					759:764	their mechanical, thermal, water uptake	726:764	their mechanical, thermal, water uptake	726:764	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	4	59	theme	methodology	644:654	arg1	impact					618:623	The impact	614:623	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties	614:788	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	0	60	theme	PCL-Based	74:82	arg1	Scaffolds					84:92	PCL-Based Scaffolds	74:92	PCL-Based Scaffolds	74:92	The Marine Polysaccharide Ulvan Confers Potent Osteoinductive Capacity to PCL-Based Scaffolds for Bone Tissue Engineering Applications.					
33802984	5	61	theme	cell	1142:1145	arg1	attachment					1147:1156	cell attachment	1142:1156	cell attachment	1142:1156	The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.					
33802984	2	62	theme	active	269:274	arg1	polysaccharide					292:305	a biologically active marine sulfated polysaccharide	254:305	a biologically active marine sulfated polysaccharide	254:305	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	2	62	theme	active	269:274	arg1	Ulvan					247:251	Ulvan	247:251	Ulvan	247:251	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	1	63	theme	synthetic	157:165	arg1	polymers					179:186	synthetic and natural polymers	157:186	synthetic and natural polymers	157:186	Hybrid composites of synthetic and natural polymers represent materials of choice for bone tissue engineering.					
33802984	4	64	theme	polysaccharide	664:677	arg1	composition					679:689	the polysaccharide composition	660:689	the polysaccharide composition	660:689	The impact of the preparation methodology and the polysaccharide composition on their morphology, as well as on their mechanical, thermal, water uptake and porosity properties was determined, while their osteoinductive potential was investigated through the evaluation of cell adhesion, viability, and osteogenic differentiation of seeded human adipose-derived mesenchymal stem cells.					
33802984	3	65	dep	ulvan	507:511	arg1	either					513:518	either	513:518	either	513:518	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	3	65	dep	ulvan	507:511	arg1	blends					532:537	blends	532:537	blends with κ-carrageenan and chondroitin sulfate	532:580	Herein, a series of hybrid polycaprolactone scaffolds containing ulvan either alone or in blends with κ-carrageenan and chondroitin sulfate was prepared and characterized.					
33802984	2	66	from	interest	328:335	arg1	development					344:354	the development	340:354	the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties	340:439	Ulvan, a biologically active marine sulfated polysaccharide, is attracting great interest in the development of novel biomedical scaffolds due to recent reports on its osteoinductive properties.					
33802984	5	67	theme	scaffolds	1236:1244	arg1	development					1210:1220	the development	1206:1220	the development of biomedical scaffolds	1206:1244	The results verified the osteoinductive ability of ulvan, showing that its incorporation into the polycaprolactone matrix efficiently promoted cell attachment and viability, thus confirming its potential in the development of biomedical scaffolds for bone tissue regeneration applications.					
34508801	4	0	theme	spectrometry	887:898	arg1	technique					916:924	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique	779:924	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique	779:924	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	2	1	theme	total	332:336	arg1	ginsenoside					338:348	total ginsenoside	332:348	total ginsenoside	332:348	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	1	2	theme	cultivated	242:251	arg1	MCG					262:264	MCG	262:264	MCG	262:264	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	1	2	theme	cultivated	242:251	arg1	ginseng					253:259	mountain cultivated ginseng	233:259	mountain cultivated ginseng (MCG)	233:265	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	4	3	theme	chromatography	841:854	arg1	spectrometry					887:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry	779:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique	779:924	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	4	3	theme	chromatography	841:854	arg1	UPLC-Q-TOF-MS					901:913	UPLC-Q-TOF-MS	901:913	UPLC-Q-TOF-MS	901:913	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	2	4	from	level	323:327	arg1	tissue					357:362	GRR tissue	353:362	GRR tissue	353:362	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	1	5	theme	occurring	157:165	arg1	disease					167:173	a commonly occurring disease	146:173	a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG)	146:265	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	1	5	theme	occurring	157:165	arg1	RELEVANCE					108:116	ETHNOPHARMACOLOGICAL RELEVANCE	87:116	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR)	87:141	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	4	6	theme	time-of-flight	867:880	arg1	spectrometry					887:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry	779:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique	779:924	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	4	6	theme	time-of-flight	867:880	arg1	UPLC-Q-TOF-MS					901:913	UPLC-Q-TOF-MS	901:913	UPLC-Q-TOF-MS	901:913	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	6	7	theme	optical	1245:1251	arg1	spectrometer					1262:1273	inductively coupled plasma optical emission spectrometer	1218:1273	inductively coupled plasma optical emission spectrometer (ICP-OES)	1218:1283	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	6	7	theme	optical	1245:1251	arg1	ICP-OES					1276:1282	ICP-OES	1276:1282	ICP-OES	1276:1282	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	6	8	theme	coupled	1230:1236	arg1	spectrometer					1262:1273	inductively coupled plasma optical emission spectrometer	1218:1273	inductively coupled plasma optical emission spectrometer (ICP-OES)	1218:1283	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	6	8	theme	coupled	1230:1236	arg1	ICP-OES					1276:1282	ICP-OES	1276:1282	ICP-OES	1276:1282	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	10	9	theme	MCG	1798:1800	arg1	ginsenosides					1770:1781	ginsenosides	1770:1781	ginsenosides of 20-year-old MCG, which further establishes reference data	1770:1842	CONCLUSIONS The results suggest that GRR affects the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data and the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR.					
34508801	3	10	theme	chemical	563:570	arg1	components					572:581	the chemical components	559:581	the chemical components in the roots of rusty	559:603	AIM OF THE STUDY This research aimed to identify differences in the chemical components in the roots of rusty compared with healthy MCG harvested at 20-years of age.					
34508801	1	11	theme	farming	203:209	arg1	value					224:228	the continuous farming and economic value	188:228	the continuous farming and economic value of mountain cultivated ginseng (MCG)	188:265	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	1	12	theme	rusty	126:130	arg1	root					132:135	rusty root	126:135	rusty root (GRR)	126:141	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	1	12	theme	rusty	126:130	arg1	GRR					138:140	GRR	138:140	GRR	138:140	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	8	13	theme	ginsenosides	1479:1490	arg1	lower					1573:1577	lower	1573:1577	lower	1573:1577	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	8	13	theme	ginsenosides	1479:1490	arg1	concentrations					1442:1455	the concentrations	1438:1455	the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd	1438:1552	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	3	14	theme	STUDY	506:510	arg1	AIM					495:497	AIM	495:497	AIM OF THE STUDY	495:510	AIM OF THE STUDY This research aimed to identify differences in the chemical components in the roots of rusty compared with healthy MCG harvested at 20-years of age.					
34508801	2	15	with	MCG	445:447	arg1	age					463:465	a higher age	454:465	a higher age	454:465	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	5	16	theme	principal	952:960	arg1	constituents					962:973	the principal constituents	948:973	the principal constituents	948:973	Chemical markers and the principal constituents were then quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).					
34508801	7	17	from	difference	1319:1328	arg1	levels					1337:1342	the levels	1333:1342	the levels of total ginsenosides or total polysaccharides	1333:1389	RESULTS There was no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups.					
34508801	0	18	theme	roots	57:61	arg1	composition					28:38	the chemical composition	15:38	the chemical composition of Panax ginseng roots	15:61	Differences in the chemical composition of Panax ginseng roots infected with red rust.					
34508801	10	19	theme	metabolic	1900:1908	arg1	changes					1910:1916	metabolic changes	1900:1916	metabolic changes in ginseng resulting from GRR	1900:1946	CONCLUSIONS The results suggest that GRR affects the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data and the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR.					
34508801	3	20	dep	aimed	526:530	arg1	AIM					495:497	AIM	495:497	AIM OF THE STUDY	495:510	AIM OF THE STUDY This research aimed to identify differences in the chemical components in the roots of rusty compared with healthy MCG harvested at 20-years of age.					
34508801	2	21	from	changes	411:417	arg1	ginsenosides					395:406	individual ginsenosides	384:406	individual ginsenosides	384:406	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	5	22	theme	Chemical	927:934	arg1	markers					936:942	Chemical markers	927:942	Chemical markers	927:942	Chemical markers and the principal constituents were then quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).					
34508801	0	23	from	Differences	0:10	arg1	composition					28:38	the chemical composition	15:38	the chemical composition of Panax ginseng roots	15:61	Differences in the chemical composition of Panax ginseng roots infected with red rust.					
34508801	5	24	theme	ultra-performance	999:1015	arg1	UPLC-MS/MS					1065:1074	UPLC-MS/MS	1065:1074	UPLC-MS/MS	1065:1074	Chemical markers and the principal constituents were then quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).					
34508801	5	24	theme	ultra-performance	999:1015	arg1	spectrometry					1051:1062	ultra-performance liquid chromatography-tandem mass spectrometry	999:1062	ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)	999:1075	Chemical markers and the principal constituents were then quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).					
34508801	4	25	theme	individual	734:743	arg1	ginsenosides					745:756	individual ginsenosides	734:756	individual ginsenosides	734:756	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	5	26	theme	chromatography-tandem	1024:1044	arg1	UPLC-MS/MS					1065:1074	UPLC-MS/MS	1065:1074	UPLC-MS/MS	1065:1074	Chemical markers and the principal constituents were then quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).					
34508801	5	26	theme	chromatography-tandem	1024:1044	arg1	spectrometry					1051:1062	ultra-performance liquid chromatography-tandem mass spectrometry	999:1062	ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)	999:1075	Chemical markers and the principal constituents were then quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).					
34508801	8	27	theme	pivotal	1460:1466	arg1	Rd					1551:1552	ginsenoside Rd	1539:1552	ginsenoside Rd	1539:1552	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	8	27	theme	pivotal	1460:1466	arg1	Rc					1515:1516	ginsenoside Rc	1503:1516	ginsenoside Rc	1503:1516	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	8	27	theme	pivotal	1460:1466	arg1	Ro					1531:1532	ginsenoside Ro	1519:1532	ginsenoside Ro	1519:1532	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	8	27	theme	pivotal	1460:1466	arg1	ginsenosides					1479:1490	pivotal individual ginsenosides	1460:1490	pivotal individual ginsenosides	1460:1490	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	10	28	from	changes	1910:1916	arg1	ginseng					1921:1927	ginseng	1921:1927	ginseng resulting from GRR	1921:1946	CONCLUSIONS The results suggest that GRR affects the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data and the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR.					
34508801	7	29	theme	healthy	1413:1419	arg1	groups					1421:1426	the rusty and healthy groups	1399:1426	the rusty and healthy groups	1399:1426	RESULTS There was no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups.					
34508801	4	30	theme	healthy	713:719	arg1	roots					725:729	healthy MCG roots	713:729	healthy MCG roots	713:729	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	9	31	theme	rusty	1659:1663	arg1	group					1665:1669	the rusty group	1655:1669	the rusty group	1655:1669	In addition, concentrations of Fe and Al were higher in the rusty group compared with the healthy group.					
34508801	7	32	theme	rusty	1403:1407	arg1	groups					1421:1426	the rusty and healthy groups	1399:1426	the rusty and healthy groups	1399:1426	RESULTS There was no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups.					
34508801	4	33	theme	non-targeted	781:792	arg1	spectrometry					887:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry	779:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique	779:924	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	4	33	theme	non-targeted	781:792	arg1	UPLC-Q-TOF-MS					901:913	UPLC-Q-TOF-MS	901:913	UPLC-Q-TOF-MS	901:913	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	2	34	theme	individual	384:393	arg1	ginsenosides					395:406	individual ginsenosides	384:406	individual ginsenosides	384:406	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	6	35	theme	total	1091:1095	arg1	ginsenosides					1097:1108	total ginsenosides	1091:1108	total ginsenosides	1091:1108	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	3	36	theme	rusty	599:603	arg1	roots					590:594	the roots	586:594	the roots of rusty	586:603	AIM OF THE STUDY This research aimed to identify differences in the chemical components in the roots of rusty compared with healthy MCG harvested at 20-years of age.					
34508801	4	37	theme	ultrahigh-performance	812:832	arg1	spectrometry					887:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry	779:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique	779:924	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	4	37	theme	ultrahigh-performance	812:832	arg1	UPLC-Q-TOF-MS					901:913	UPLC-Q-TOF-MS	901:913	UPLC-Q-TOF-MS	901:913	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	10	38	theme	20-year-old	1786:1796	arg1	MCG					1798:1800	20-year-old MCG	1786:1800	20-year-old MCG	1786:1800	CONCLUSIONS The results suggest that GRR affects the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data and the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR.					
34508801	0	39	theme	Panax	43:47	arg1	roots					57:61	Panax ginseng roots	43:61	Panax ginseng roots	43:61	Differences in the chemical composition of Panax ginseng roots infected with red rust.					
34508801	2	40	from	differences	369:379	arg1	ginsenosides					395:406	individual ginsenosides	384:406	individual ginsenosides	384:406	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	7	41	theme	total	1369:1373	arg1	polysaccharides					1375:1389	total polysaccharides	1369:1389	total polysaccharides	1369:1389	RESULTS There was no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups.					
34508801	7	42	dep	RESULTS	1286:1292	arg1	was					1300:1302	was	1300:1302	was no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups	1300:1426	RESULTS There was no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups.					
34508801	8	43	theme	ginsenoside	1519:1529	arg1	Ro					1531:1532	ginsenoside Ro	1519:1532	ginsenoside Ro	1519:1532	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	10	44	theme	ginsenosides	1770:1781	arg1	basis					1852:1856	the basis	1848:1856	the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR	1848:1946	CONCLUSIONS The results suggest that GRR affects the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data and the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR.					
34508801	10	44	theme	ginsenosides	1770:1781	arg1	synthesis					1757:1765	the synthesis	1753:1765	the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data	1753:1842	CONCLUSIONS The results suggest that GRR affects the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data and the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR.					
34508801	7	45	theme	ginsenosides	1353:1364	arg1	levels					1337:1342	the levels	1333:1342	the levels of total ginsenosides or total polysaccharides	1333:1389	RESULTS There was no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups.					
34508801	2	46	theme	GRR	353:355	arg1	tissue					357:362	GRR tissue	353:362	GRR tissue	353:362	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	2	47	theme	ginsenoside	338:348	arg1	level					323:327	a generally smaller level	303:327	a generally smaller level of total ginsenoside in GRR tissue	303:362	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	1	48	theme	mountain	233:240	arg1	MCG					262:264	MCG	262:264	MCG	262:264	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	1	48	theme	mountain	233:240	arg1	ginseng					253:259	mountain cultivated ginseng	233:259	mountain cultivated ginseng (MCG)	233:265	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	8	49	theme	rusty	1586:1590	arg1	group					1592:1596	the rusty group	1582:1596	the rusty group	1582:1596	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	4	50	theme	mass	882:885	arg1	spectrometry					887:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry	779:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique	779:924	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	4	50	theme	mass	882:885	arg1	UPLC-Q-TOF-MS					901:913	UPLC-Q-TOF-MS	901:913	UPLC-Q-TOF-MS	901:913	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	6	51	theme	elemental	1142:1150	arg1	composition					1152:1162	the elemental composition	1138:1162	the elemental composition	1138:1162	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	10	52	dep	CONCLUSIONS	1704:1714	arg1	suggest					1728:1734	suggest	1728:1734	suggest that GRR affects the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data and the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR	1728:1946	CONCLUSIONS The results suggest that GRR affects the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data and the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR.					
34508801	2	53	theme	Previous	268:275	arg1	studies					277:283	Previous studies	268:283	Previous studies	268:283	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	1	54	theme	ginseng	253:259	arg1	value					224:228	the continuous farming and economic value	188:228	the continuous farming and economic value of mountain cultivated ginseng (MCG)	188:265	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	10	55	theme	reference	1829:1837	arg1	data					1839:1842	reference data	1829:1842	reference data	1829:1842	CONCLUSIONS The results suggest that GRR affects the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data and the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR.					
34508801	4	56	theme	liquid	834:839	arg1	spectrometry					887:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry	779:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique	779:924	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	4	56	theme	liquid	834:839	arg1	UPLC-Q-TOF-MS					901:913	UPLC-Q-TOF-MS	901:913	UPLC-Q-TOF-MS	901:913	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	6	57	theme	emission	1253:1260	arg1	spectrometer					1262:1273	inductively coupled plasma optical emission spectrometer	1218:1273	inductively coupled plasma optical emission spectrometer (ICP-OES)	1218:1283	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	6	57	theme	emission	1253:1260	arg1	ICP-OES					1276:1282	ICP-OES	1276:1282	ICP-OES	1276:1282	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	4	58	theme	quadrupole	856:865	arg1	spectrometry					887:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry	779:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique	779:924	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	4	58	theme	quadrupole	856:865	arg1	UPLC-Q-TOF-MS					901:913	UPLC-Q-TOF-MS	901:913	UPLC-Q-TOF-MS	901:913	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	6	59	theme	plasma	1238:1243	arg1	spectrometer					1262:1273	inductively coupled plasma optical emission spectrometer	1218:1273	inductively coupled plasma optical emission spectrometer (ICP-OES)	1218:1283	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	6	59	theme	plasma	1238:1243	arg1	ICP-OES					1276:1282	ICP-OES	1276:1282	ICP-OES	1276:1282	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	4	60	theme	metabonomic-based	794:810	arg1	spectrometry					887:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry	779:898	a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique	779:924	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	4	60	theme	metabonomic-based	794:810	arg1	UPLC-Q-TOF-MS					901:913	UPLC-Q-TOF-MS	901:913	UPLC-Q-TOF-MS	901:913	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	8	61	theme	ginsenoside	1539:1549	arg1	Rd					1551:1552	ginsenoside Rd	1539:1552	ginsenoside Rd	1539:1552	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	1	62	theme	continuous	192:201	arg1	value					224:228	the continuous farming and economic value	188:228	the continuous farming and economic value of mountain cultivated ginseng (MCG)	188:265	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	4	63	theme	MATERIALS	661:669	arg1	Differences					683:693	MATERIALS AND METHODS Differences	661:693	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides	661:756	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	9	64	theme	healthy	1689:1695	arg1	group					1697:1701	the healthy group	1685:1701	the healthy group	1685:1701	In addition, concentrations of Fe and Al were higher in the rusty group compared with the healthy group.					
34508801	2	65	with	rusty	427:431	arg1	age					463:465	a higher age	454:465	a higher age	454:465	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	4	66	theme	METHODS	675:681	arg1	Differences					683:693	MATERIALS AND METHODS Differences	661:693	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides	661:756	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	2	67	theme	smaller	315:321	arg1	level					323:327	a generally smaller level	303:327	a generally smaller level of total ginsenoside in GRR tissue	303:362	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	7	68	theme	significant	1307:1317	arg1	difference					1319:1328	no significant difference	1304:1328	no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups	1304:1426	RESULTS There was no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups.					
34508801	0	69	theme	red	77:79	arg1	rust					81:84	red rust	77:84	red rust	77:84	Differences in the chemical composition of Panax ginseng roots infected with red rust.					
34508801	2	70	theme	higher	456:461	arg1	age					463:465	a higher age	454:465	a higher age	454:465	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	9	71	theme	Fe	1630:1631	arg1	concentrations					1612:1625	concentrations	1612:1625	concentrations of Fe and Al	1612:1638	In addition, concentrations of Fe and Al were higher in the rusty group compared with the healthy group.					
34508801	4	72	theme	MCG	721:723	arg1	roots					725:729	healthy MCG roots	713:729	healthy MCG roots	713:729	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	3	73	from	components	572:581	arg1	roots					590:594	the roots	586:594	the roots of rusty	586:603	AIM OF THE STUDY This research aimed to identify differences in the chemical components in the roots of rusty compared with healthy MCG harvested at 20-years of age.					
34508801	5	74	theme	liquid	1017:1022	arg1	UPLC-MS/MS					1065:1074	UPLC-MS/MS	1065:1074	UPLC-MS/MS	1065:1074	Chemical markers and the principal constituents were then quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).					
34508801	5	74	theme	liquid	1017:1022	arg1	spectrometry					1051:1062	ultra-performance liquid chromatography-tandem mass spectrometry	999:1062	ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)	999:1075	Chemical markers and the principal constituents were then quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).					
34508801	3	75	from	differences	544:554	arg1	components					572:581	the chemical components	559:581	the chemical components in the roots of rusty	559:603	AIM OF THE STUDY This research aimed to identify differences in the chemical components in the roots of rusty compared with healthy MCG harvested at 20-years of age.					
34508801	0	76	theme	chemical	19:26	arg1	composition					28:38	the chemical composition	15:38	the chemical composition of Panax ginseng roots	15:61	Differences in the chemical composition of Panax ginseng roots infected with red rust.					
34508801	1	77	theme	ETHNOPHARMACOLOGICAL	87:106	arg1	disease					167:173	a commonly occurring disease	146:173	a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG)	146:265	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	1	77	theme	ETHNOPHARMACOLOGICAL	87:106	arg1	RELEVANCE					108:116	ETHNOPHARMACOLOGICAL RELEVANCE	87:116	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR)	87:141	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	6	78	theme	total	1111:1115	arg1	polysaccharides					1117:1131	total polysaccharides	1111:1131	total polysaccharides	1111:1131	Furthermore, total ginsenosides, total polysaccharides, and the elemental composition were evaluated separately using spectrophotometry and inductively coupled plasma optical emission spectrometer (ICP-OES).					
34508801	9	79	theme	Al	1637:1638	arg1	concentrations					1612:1625	concentrations	1612:1625	concentrations of Fe and Al	1612:1638	In addition, concentrations of Fe and Al were higher in the rusty group compared with the healthy group.					
34508801	8	80	theme	individual	1468:1477	arg1	Rd					1551:1552	ginsenoside Rd	1539:1552	ginsenoside Rd	1539:1552	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	8	80	theme	individual	1468:1477	arg1	Rc					1515:1516	ginsenoside Rc	1503:1516	ginsenoside Rc	1503:1516	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	8	80	theme	individual	1468:1477	arg1	Ro					1531:1532	ginsenoside Ro	1519:1532	ginsenoside Ro	1519:1532	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	8	80	theme	individual	1468:1477	arg1	ginsenosides					1479:1490	pivotal individual ginsenosides	1460:1490	pivotal individual ginsenosides	1460:1490	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	7	81	theme	polysaccharides	1375:1389	arg1	levels					1337:1342	the levels	1333:1342	the levels of total ginsenosides or total polysaccharides	1333:1389	RESULTS There was no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups.					
34508801	0	82	theme	ginseng	49:55	arg1	roots					57:61	Panax ginseng roots	43:61	Panax ginseng roots	43:61	Differences in the chemical composition of Panax ginseng roots infected with red rust.					
34508801	2	83	theme	healthy	437:443	arg1	MCG					445:447	healthy MCG	437:447	healthy MCG	437:447	Previous studies have demonstrated a generally smaller level of total ginsenoside in GRR tissue, but differences in individual ginsenosides or changes between rusty and healthy MCG with a higher age have not been investigated.					
34508801	8	84	theme	ginsenoside	1503:1513	arg1	Rc					1515:1516	ginsenoside Rc	1503:1516	ginsenoside Rc	1503:1516	However, the concentrations of pivotal individual ginsenosides, including ginsenoside Rc, ginsenoside Ro, and ginsenoside Rd were significantly lower in the rusty group.					
34508801	4	85	from	Differences	683:693	arg1	ginsenosides					745:756	individual ginsenosides	734:756	individual ginsenosides	734:756	MATERIALS AND METHODS Differences between rusty and healthy MCG roots in individual ginsenosides were evaluated using a non-targeted metabonomic-based ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) technique.					
34508801	5	86	theme	mass	1046:1049	arg1	UPLC-MS/MS					1065:1074	UPLC-MS/MS	1065:1074	UPLC-MS/MS	1065:1074	Chemical markers and the principal constituents were then quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).					
34508801	5	86	theme	mass	1046:1049	arg1	spectrometry					1051:1062	ultra-performance liquid chromatography-tandem mass spectrometry	999:1062	ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)	999:1075	Chemical markers and the principal constituents were then quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).					
34508801	10	87	theme	mechanisms	1881:1890	arg1	exploration					1862:1872	exploration	1862:1872	exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR	1862:1946	CONCLUSIONS The results suggest that GRR affects the synthesis of ginsenosides of 20-year-old MCG, which further establishes reference data and the basis for exploration of the mechanisms causing metabolic changes in ginseng resulting from GRR.					
34508801	7	88	theme	total	1347:1351	arg1	ginsenosides					1353:1364	total ginsenosides	1347:1364	total ginsenosides	1347:1364	RESULTS There was no significant difference in the levels of total ginsenosides or total polysaccharides between the rusty and healthy groups.					
34508801	3	89	theme	healthy	619:625	arg1	MCG					627:629	healthy MCG	619:629	healthy MCG harvested at 20-years of age	619:658	AIM OF THE STUDY This research aimed to identify differences in the chemical components in the roots of rusty compared with healthy MCG harvested at 20-years of age.					
34508801	1	90	theme	economic	215:222	arg1	value					224:228	the continuous farming and economic value	188:228	the continuous farming and economic value of mountain cultivated ginseng (MCG)	188:265	ETHNOPHARMACOLOGICAL RELEVANCE Ginseng rusty root (GRR) is a commonly occurring disease that affects the continuous farming and economic value of mountain cultivated ginseng (MCG).					
34508801	3	91	theme	age	656:658	arg1	20-years					644:651	20-years	644:651	20-years of age	644:658	AIM OF THE STUDY This research aimed to identify differences in the chemical components in the roots of rusty compared with healthy MCG harvested at 20-years of age.					
33729756	5	0	theme	interfacial	808:818	arg1	characterizations					820:836	interfacial characterizations	808:836	interfacial characterizations	808:836	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	2	1	from	application	459:469	arg1	healing					480:486	wound healing	474:486	wound healing	474:486	However, there is still a demand for developing a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing.					
33729756	7	2	theme	effective	1333:1341	arg1	material					1379:1386	an effective and easily available wound dressing material	1330:1386	an effective and easily available wound dressing material	1330:1386	These progresses suggested a prospect for the PEP-PAM hydrogel as an effective and easily available wound dressing material.					
33729756	3	3	from	prolifera	584:592	arg1	polysaccharides					550:564	water-soluble polysaccharides	536:564	water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM)	536:684	Herein, a composite hydrogel was prepared with water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM) using a one-pot method.					
33729756	0	4	theme	Facile	93:98	arg1	Transformation					100:113	A Facile Transformation	91:113	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.	0:158	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	8	5	theme	biomass	1614:1620	arg1	utilization					1572:1582	the high-value utilization	1557:1582	the high-value utilization of disastrous marine blooming biomass	1557:1620	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	2	6	theme	chemical	381:388	arg1	modifications					390:402	extra chemical modifications	375:402	extra chemical modifications	375:402	However, there is still a demand for developing a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing.					
33729756	8	7	theme	new	1543:1545	arg1	route					1547:1551	a new route	1541:1551	a new route for the high-value utilization of disastrous marine blooming biomass	1541:1620	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	0	8	theme	Marine	118:123	arg1	Blooming					125:132	Marine Blooming	118:132	Marine Blooming	118:132	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	8	9	theme	blooming	1605:1612	arg1	biomass					1614:1620	disastrous marine blooming biomass	1587:1620	disastrous marine blooming biomass	1587:1620	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	2	10	theme	dressing	300:307	arg1	hydrogel					309:316	a wound dressing hydrogel	292:316	a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing	292:486	However, there is still a demand for developing a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing.					
33729756	4	11	theme	mechanical	777:786	arg1	strength					788:795	an ultratough mechanical strength	763:795	an ultratough mechanical strength	763:795	The dual-network of this hydrogel enabled it to have an ultratough mechanical strength.					
33729756	4	12	contain	have	758:761	arg1	it					752:753	it	752:753	it	752:753	The dual-network of this hydrogel enabled it to have an ultratough mechanical strength.					
33729756	4	12	contain	have	758:761	arg2	strength					788:795	an ultratough mechanical strength	763:795	an ultratough mechanical strength	763:795	The dual-network of this hydrogel enabled it to have an ultratough mechanical strength.					
33729756	8	13	theme	dressing	1434:1441	arg1	hydrogel					1443:1450	a wound dressing hydrogel	1426:1450	a wound dressing hydrogel	1426:1450	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	2	14	theme	wound	294:298	arg1	hydrogel					309:316	a wound dressing hydrogel	292:316	a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing	292:486	However, there is still a demand for developing a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing.					
33729756	7	15	theme	dressing	1370:1377	arg1	material					1379:1386	an effective and easily available wound dressing material	1330:1386	an effective and easily available wound dressing material	1330:1386	These progresses suggested a prospect for the PEP-PAM hydrogel as an effective and easily available wound dressing material.					
33729756	4	16	theme	hydrogel	735:742	arg1	dual-network					714:725	The dual-network	710:725	The dual-network of this hydrogel	710:742	The dual-network of this hydrogel enabled it to have an ultratough mechanical strength.					
33729756	3	17	theme	Enteromorpha	571:582	arg1	PEP					595:597	PEP	595:597	PEP	595:597	Herein, a composite hydrogel was prepared with water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM) using a one-pot method.					
33729756	3	17	theme	Enteromorpha	571:582	arg1	prolifera					584:592	Enteromorpha prolifera	571:592	Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM)	571:684	Herein, a composite hydrogel was prepared with water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM) using a one-pot method.					
33729756	5	18	theme	self-healing	925:936	arg1	ability					938:944	the self-healing ability	921:944	the self-healing ability of the PEP-PAM hydrogel	921:968	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	0	19	theme	Blooming	125:132	arg1	Transformation					100:113	A Facile Transformation	91:113	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.	0:158	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	5	20	from	groups	987:992	arg1	hydrogel					1001:1008	the hydrogel	997:1008	the hydrogel	997:1008	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	0	21	theme	Wound	0:4	arg1	Hydrogel					15:22	Wound Dressing Hydrogel	0:22	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.	0:158	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	0	22	theme	Dressing	6:13	arg1	Hydrogel					15:22	Wound Dressing Hydrogel	0:22	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.	0:158	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	2	23	theme	natural	334:340	arg1	macromolecules					342:355	natural macromolecules	334:355	natural macromolecules	334:355	However, there is still a demand for developing a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing.					
33729756	2	24	theme	wound	474:478	arg1	healing					480:486	wound healing	474:486	wound healing	474:486	However, there is still a demand for developing a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing.					
33729756	8	25	theme	biomedical	1648:1657	arg1	material					1659:1666	a biomedical material	1646:1666	a biomedical material	1646:1666	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	7	26	theme	PEP-PAM	1310:1316	arg1	hydrogel					1318:1325	the PEP-PAM hydrogel	1306:1325	the PEP-PAM hydrogel as an effective and easily available wound dressing material	1306:1386	These progresses suggested a prospect for the PEP-PAM hydrogel as an effective and easily available wound dressing material.					
33729756	3	27	theme	boric	618:622	arg1	acid					624:627	boric acid	618:627	boric acid	618:627	Herein, a composite hydrogel was prepared with water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM) using a one-pot method.					
33729756	6	28	theme	cell	1155:1158	arg1	proliferation					1160:1172	cell proliferation	1155:1172	cell proliferation	1155:1172	Additionally, human epidermal growth factor-loaded PEP-PAM hydrogel promoted cell proliferation and migration in vitro and significantly accelerated wound healing in vivo on model rats.					
33729756	5	29	theme	dynamic	876:882	arg1	bonds					900:904	dynamic hydroxyl-borate bonds	876:904	dynamic hydroxyl-borate bonds	876:904	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	6	30	theme	PEP-PAM	1129:1135	arg1	hydrogel					1137:1144	human epidermal growth factor-loaded PEP-PAM hydrogel	1092:1144	human epidermal growth factor-loaded PEP-PAM hydrogel	1092:1144	Additionally, human epidermal growth factor-loaded PEP-PAM hydrogel promoted cell proliferation and migration in vitro and significantly accelerated wound healing in vivo on model rats.					
33729756	1	31	theme	wound	218:222	arg1	materials					233:241	wound dressing materials	218:241	wound dressing materials	218:241	Great endeavors have been dedicated to the development of wound dressing materials.					
33729756	4	32	theme	ultratough	766:775	arg1	strength					788:795	an ultratough mechanical strength	763:795	an ultratough mechanical strength	763:795	The dual-network of this hydrogel enabled it to have an ultratough mechanical strength.					
33729756	0	33	theme	Biomedical	139:148	arg1	Material					150:157	Biomedical Material	139:157	Biomedical Material	139:157	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	8	34	theme	natural	1487:1493	arg1	component					1520:1528	a one component	1514:1528	a one component	1514:1528	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	8	34	theme	natural	1487:1493	arg1	polysaccharides					1495:1509	natural polysaccharides	1487:1509	natural polysaccharides	1487:1509	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	3	35	theme	water-soluble	536:548	arg1	polysaccharides					550:564	water-soluble polysaccharides	536:564	water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM)	536:684	Herein, a composite hydrogel was prepared with water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM) using a one-pot method.					
33729756	1	36	theme	dressing	224:231	arg1	materials					233:241	wound dressing materials	218:241	wound dressing materials	218:241	Great endeavors have been dedicated to the development of wound dressing materials.					
33729756	0	37	theme	prolifera	40:48	arg1	Composite					80:88	Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite	27:88	Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite	27:88	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	5	38	theme	tissue	1022:1027	arg1	adhesiveness					1029:1040	tissue adhesiveness	1022:1040	tissue adhesiveness	1022:1040	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	3	39	theme	one-pot	694:700	arg1	method					702:707	a one-pot method	692:707	a one-pot method	692:707	Herein, a composite hydrogel was prepared with water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM) using a one-pot method.					
33729756	1	40	theme	materials	233:241	arg1	development					203:213	the development	199:213	the development of wound dressing materials	199:241	Great endeavors have been dedicated to the development of wound dressing materials.					
33729756	0	41	theme	Enteromorpha	27:38	arg1	Composite					80:88	Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite	27:88	Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite	27:88	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	6	42	theme	human	1092:1096	arg1	hydrogel					1137:1144	human epidermal growth factor-loaded PEP-PAM hydrogel	1092:1144	human epidermal growth factor-loaded PEP-PAM hydrogel	1092:1144	Additionally, human epidermal growth factor-loaded PEP-PAM hydrogel promoted cell proliferation and migration in vitro and significantly accelerated wound healing in vivo on model rats.					
33729756	2	43	theme	facile	423:428	arg1	transformation					430:443	a facile transformation	421:443	a facile transformation	421:443	However, there is still a demand for developing a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing.					
33729756	0	44	theme	Composite	80:88	arg1	Hydrogel					15:22	Wound Dressing Hydrogel	0:22	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.	0:158	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	5	45	theme	hydrogen	857:864	arg1	bonds					866:870	the hydrogen bonds	853:870	the hydrogen bonds	853:870	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	8	46	theme	disastrous	1587:1596	arg1	biomass					1614:1620	disastrous marine blooming biomass	1587:1620	disastrous marine blooming biomass	1587:1620	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	2	47	theme	extra	375:379	arg1	modifications					390:402	extra chemical modifications	375:402	extra chemical modifications	375:402	However, there is still a demand for developing a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing.					
33729756	0	48	theme	Polysaccharide-Polyacrylamide	50:78	arg1	Composite					80:88	Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite	27:88	Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite	27:88	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	8	49	theme	high-value	1561:1570	arg1	utilization					1572:1582	the high-value utilization	1557:1582	the high-value utilization of disastrous marine blooming biomass	1557:1620	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	5	50	theme	surface	979:985	arg1	groups					987:992	the surface groups	975:992	the surface groups on the hydrogel	975:1008	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	7	51	theme	available	1354:1362	arg1	material					1379:1386	an effective and easily available wound dressing material	1330:1386	an effective and easily available wound dressing material	1330:1386	These progresses suggested a prospect for the PEP-PAM hydrogel as an effective and easily available wound dressing material.					
33729756	5	52	theme	antioxidant	1054:1064	arg1	properties					1066:1075	natural antioxidant properties	1046:1075	natural antioxidant properties	1046:1075	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	6	53	theme	epidermal	1098:1106	arg1	hydrogel					1137:1144	human epidermal growth factor-loaded PEP-PAM hydrogel	1092:1144	human epidermal growth factor-loaded PEP-PAM hydrogel	1092:1144	Additionally, human epidermal growth factor-loaded PEP-PAM hydrogel promoted cell proliferation and migration in vitro and significantly accelerated wound healing in vivo on model rats.					
33729756	3	54	theme	composite	499:507	arg1	hydrogel					509:516	a composite hydrogel	497:516	a composite hydrogel	497:516	Herein, a composite hydrogel was prepared with water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM) using a one-pot method.					
33729756	6	55	theme	growth	1108:1113	arg1	hydrogel					1137:1144	human epidermal growth factor-loaded PEP-PAM hydrogel	1092:1144	human epidermal growth factor-loaded PEP-PAM hydrogel	1092:1144	Additionally, human epidermal growth factor-loaded PEP-PAM hydrogel promoted cell proliferation and migration in vitro and significantly accelerated wound healing in vivo on model rats.					
33729756	5	56	theme	natural	1046:1052	arg1	properties					1066:1075	natural antioxidant properties	1046:1075	natural antioxidant properties	1046:1075	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	0	57	dep	Hydrogel	15:22	arg1	Transformation					100:113	A Facile Transformation	91:113	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.	0:158	Wound Dressing Hydrogel of Enteromorpha prolifera Polysaccharide-Polyacrylamide Composite: A Facile Transformation of Marine Blooming into Biomedical Material.					
33729756	2	58	from	transformation	430:443	arg1	healing					480:486	wound healing	474:486	wound healing	474:486	However, there is still a demand for developing a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing.					
33729756	1	59	theme	Great	160:164	arg1	endeavors					166:174	Great endeavors	160:174	Great endeavors	160:174	Great endeavors have been dedicated to the development of wound dressing materials.					
33729756	8	60	theme	wound	1428:1432	arg1	hydrogel					1443:1450	a wound dressing hydrogel	1426:1450	a wound dressing hydrogel	1426:1450	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	6	61	theme	wound	1227:1231	arg1	healing					1233:1239	wound healing	1227:1239	wound healing	1227:1239	Additionally, human epidermal growth factor-loaded PEP-PAM hydrogel promoted cell proliferation and migration in vitro and significantly accelerated wound healing in vivo on model rats.					
33729756	6	62	theme	factor-loaded	1115:1127	arg1	hydrogel					1137:1144	human epidermal growth factor-loaded PEP-PAM hydrogel	1092:1144	human epidermal growth factor-loaded PEP-PAM hydrogel	1092:1144	Additionally, human epidermal growth factor-loaded PEP-PAM hydrogel promoted cell proliferation and migration in vitro and significantly accelerated wound healing in vivo on model rats.					
33729756	2	63	theme	practical	449:457	arg1	application					459:469	practical application	449:469	practical application in wound healing	449:486	However, there is still a demand for developing a wound dressing hydrogel that integrates natural macromolecules without requiring extra chemical modifications, so as to enable a facile transformation and practical application in wound healing.					
33729756	5	64	theme	PEP-PAM	953:959	arg1	hydrogel					961:968	the PEP-PAM hydrogel	949:968	the PEP-PAM hydrogel	949:968	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	6	65	theme	model	1252:1256	arg1	rats					1258:1261	model rats	1252:1261	model rats	1252:1261	Additionally, human epidermal growth factor-loaded PEP-PAM hydrogel promoted cell proliferation and migration in vitro and significantly accelerated wound healing in vivo on model rats.					
33729756	8	66	theme	marine	1598:1603	arg1	biomass					1614:1620	disastrous marine blooming biomass	1587:1620	disastrous marine blooming biomass	1587:1620	Remarkably, this work showcases that a wound dressing hydrogel can be facially developed by using natural polysaccharides as a one component and offers a new route for the high-value utilization of disastrous marine blooming biomass by transforming it into a biomedical material.					
33729756	5	67	theme	hydroxyl-borate	884:898	arg1	bonds					900:904	dynamic hydroxyl-borate bonds	876:904	dynamic hydroxyl-borate bonds	876:904	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	7	68	theme	wound	1364:1368	arg1	material					1379:1386	an effective and easily available wound dressing material	1330:1386	an effective and easily available wound dressing material	1330:1386	These progresses suggested a prospect for the PEP-PAM hydrogel as an effective and easily available wound dressing material.					
33729756	5	69	theme	hydrogel	961:968	arg1	ability					938:944	the self-healing ability	921:944	the self-healing ability of the PEP-PAM hydrogel	921:968	Moreover, interfacial characterizations reflected that the hydrogen bonds and dynamic hydroxyl-borate bonds contributed to the self-healing ability of the PEP-PAM hydrogel, and the surface groups on the hydrogel allowed for tissue adhesiveness and natural antioxidant properties.					
33729756	3	70	attach	cross-linked	648:659	arg3	PAM					681:683	PAM	681:683	PAM	681:683	Herein, a composite hydrogel was prepared with water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM) using a one-pot method.					
33729756	3	70	attach	cross-linked	648:659	arg3	polymerization					665:678	polymerization	665:678	polymerization (PAM)	665:684	Herein, a composite hydrogel was prepared with water-soluble polysaccharides from Enteromorpha prolifera (PEP) cross-linked with boric acid and polyacrylamide cross-linked via polymerization (PAM) using a one-pot method.					
33142621	7	0	theme	active	1070:1075	arg1	PKP-E-2-1					1036:1044	The PKP-E-2-1	1032:1044	The PKP-E-2-1	1032:1044	The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.					
33142621	7	0	theme	active	1070:1075	arg1	fraction					1077:1084	the most stable and active fraction	1050:1084	the most stable and active fraction	1050:1084	The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.					
33142621	6	1	theme	radical	1008:1014	arg1	tests					1025:1029	scavenging radical capacity tests	997:1029	scavenging radical capacity tests	997:1029	Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests.					
33142621	3	2	theme	periodate	490:498	arg1	HPGPC					430:434	HPGPC	430:434	HPGPC	430:434	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	3	2	theme	periodate	490:498	arg1	oxidation					500:508	periodate oxidation	490:508	periodate oxidation	490:508	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	6	3	theme	antioxidant	934:944	arg1	activities					946:955	the antioxidant activities	930:955	the antioxidant activities of the polysaccharides	930:978	Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests.					
33142621	0	4	theme	Pinus	96:100	arg1	koraiensis					102:111	Pinus koraiensis	96:111	Pinus koraiensis	96:111	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	7	5	theme	stable	1059:1064	arg1	PKP-E-2-1					1036:1044	The PKP-E-2-1	1032:1044	The PKP-E-2-1	1032:1044	The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.					
33142621	7	5	theme	stable	1059:1064	arg1	fraction					1077:1084	the most stable and active fraction	1050:1084	the most stable and active fraction	1050:1084	The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.					
33142621	2	6	theme	polysaccharide	283:296	arg1	fractions					298:306	Four novel polysaccharide fractions	272:306	Four novel polysaccharide fractions	272:306	Four novel polysaccharide fractions were obtained, which were PKP-E-1-1, -1-2, -2-1, and -2-2, respectively.					
33142621	1	7	theme	Sephadex	256:263	arg1	G-100					265:269	Sephadex G-100	256:269	Sephadex G-100	256:269	The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100.					
33142621	7	8	theme	ABTS·+	1132:1137	arg1	radicals					1139:1146	the hydroxyl and ABTS·+ radicals	1115:1146	radicals	1139:1146	The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.					
33142621	2	9	theme	novel	277:281	arg1	fractions					298:306	Four novel polysaccharide fractions	272:306	Four novel polysaccharide fractions	272:306	Four novel polysaccharide fractions were obtained, which were PKP-E-1-1, -1-2, -2-1, and -2-2, respectively.					
33142621	3	10	theme	FTIR	530:533	arg1	HPGPC					430:434	HPGPC	430:434	HPGPC	430:434	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	3	10	theme	FTIR	530:533	arg1	spectroscopy					543:554	FTIR and NMR spectroscopy	530:554	spectroscopy	543:554	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	6	11	theme	scavenging	997:1006	arg1	tests					1025:1029	scavenging radical capacity tests	997:1029	scavenging radical capacity tests	997:1029	Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests.					
33142621	3	12	theme	Smith	511:515	arg1	HPGPC					430:434	HPGPC	430:434	HPGPC	430:434	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	3	12	theme	Smith	511:515	arg1	degradation					517:527	Smith degradation	511:527	Smith degradation	511:527	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	0	13	theme	koraiensis	102:111	arg1	pinecones					83:91	pinecones	83:91	pinecones of Pinus koraiensis	83:111	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	5	14	theme	1→6	774:776	arg1	bonds					795:799	1→6 or 1→ glycosidic bonds	774:799	1→6 or 1→ glycosidic bonds	774:799	The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin.					
33142621	5	15	dep	-β-d-Man-	863:871	arg1	1→					891:892	1→	891:892	1→	891:892	The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin.					
33142621	5	15	dep	-β-d-Man-	863:871	arg1	α-d-GalpA-					880:889	α-d-GalpA-	880:889	α-d-GalpA-	880:889	The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin.					
33142621	5	15	dep	-β-d-Man-	863:871	arg1	1→					873:874	1→	873:874	1→	873:874	The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin.					
33142621	7	16	theme	hydroxyl	1119:1126	arg1	radicals					1139:1146	the hydroxyl and ABTS·+ radicals	1115:1146	radicals	1139:1146	The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.					
33142621	1	17	theme	koraiensis	180:189	arg1	pinecones					161:169	the pinecones	157:169	the pinecones of Pinus koraiensis	157:189	The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100.					
33142621	4	18	theme	helical	617:623	arg1	heteropolysaccharides					636:656	non-triple helical structured heteropolysaccharides	606:656	non-triple helical structured heteropolysaccharides	606:656	The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose.					
33142621	0	19	from	pinecones	83:91	arg1	purification					11:22	purification	11:22	purification	11:22	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	0	19	from	pinecones	83:91	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	0	19	from	pinecones	83:91	arg1	structure					25:33	structure	25:33	structure	25:33	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	0	19	from	pinecones	83:91	arg1	activity					51:58	antioxidant activity	39:58	antioxidant activity	39:58	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	3	20	theme	NMR	539:541	arg1	HPGPC					430:434	HPGPC	430:434	HPGPC	430:434	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	3	20	theme	NMR	539:541	arg1	spectroscopy					543:554	FTIR and NMR spectroscopy	530:554	spectroscopy	543:554	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	4	21	theme	non-triple	606:615	arg1	heteropolysaccharides					636:656	non-triple helical structured heteropolysaccharides	606:656	non-triple helical structured heteropolysaccharides	606:656	The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose.					
33142621	3	22	theme	structural	385:394	arg1	features					396:403	The structural features	381:403	The structural features	381:403	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	4	23	dep	showed	569:574	arg1	composed					662:669	composed	662:669	composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose	662:735	The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose.					
33142621	4	23	dep	showed	569:574	arg1	heteropolysaccharides					636:656	non-triple helical structured heteropolysaccharides	606:656	non-triple helical structured heteropolysaccharides	606:656	The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose.					
33142621	6	24	theme	capacity	1016:1023	arg1	tests					1025:1029	scavenging radical capacity tests	997:1029	scavenging radical capacity tests	997:1029	Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests.					
33142621	5	25	theme	fractions	823:831	arg1	backbone					809:816	the backbone	805:816	the backbone of 4 fractions	805:831	The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin.					
33142621	7	26	theme	respective	1095:1104	arg1	IC50					1106:1109	the respective IC50	1091:1109	the respective IC50 for the hydroxyl and ABTS·+ radicals	1091:1146	The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.					
33142621	7	26	theme	respective	1095:1104	arg1	mg/mL					1166:1170	3.0 and 23.6 mg/mL	1153:1170	3.0 and 23.6 mg/mL	1153:1170	The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.					
33142621	4	27	theme	structured	625:634	arg1	heteropolysaccharides					636:656	non-triple helical structured heteropolysaccharides	606:656	non-triple helical structured heteropolysaccharides	606:656	The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose.					
33142621	5	28	theme	1→	781:782	arg1	bonds					795:799	1→6 or 1→ glycosidic bonds	774:799	1→6 or 1→ glycosidic bonds	774:799	The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin.					
33142621	5	29	theme	glycosidic	784:793	arg1	bonds					795:799	1→6 or 1→ glycosidic bonds	774:799	1→6 or 1→ glycosidic bonds	774:799	The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin.					
33142621	3	30	theme	red	480:482	arg1	HPGPC					430:434	HPGPC	430:434	HPGPC	430:434	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	3	30	theme	red	480:482	arg1	test					484:487	Congo red test	474:487	Congo red test	474:487	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	0	31	theme	antioxidant	39:49	arg1	activity					51:58	antioxidant activity	39:58	antioxidant activity	39:58	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	5	32	attach	linked	764:769	arg3	bonds					795:799	1→6 or 1→ glycosidic bonds	774:799	1→6 or 1→ glycosidic bonds	774:799	The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin.					
33142621	5	32	attach	linked	764:769	arg2	fractions					742:750	The fractions	738:750	The fractions	738:750	The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin.					
33142621	4	33	theme	purified	582:589	arg1	fractions					591:599	the 4 purified fractions	576:599	the 4 purified fractions	576:599	The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose.					
33142621	6	34	theme	polysaccharides	964:978	arg1	activities					946:955	the antioxidant activities	930:955	the antioxidant activities of the polysaccharides	930:978	Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests.					
33142621	0	35	theme	polysaccharide	63:76	arg1	purification					11:22	purification	11:22	purification	11:22	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	0	35	theme	polysaccharide	63:76	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	0	35	theme	polysaccharide	63:76	arg1	structure					25:33	structure	25:33	structure	25:33	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	0	35	theme	polysaccharide	63:76	arg1	activity					51:58	antioxidant activity	39:58	antioxidant activity	39:58	Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis.					
33142621	3	36	theme	monosaccharide	437:450	arg1	HPGPC					430:434	HPGPC	430:434	HPGPC	430:434	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	3	36	theme	monosaccharide	437:450	arg1	analysis					464:471	monosaccharide composition analysis	437:471	monosaccharide composition analysis	437:471	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	3	37	theme	composition	452:462	arg1	HPGPC					430:434	HPGPC	430:434	HPGPC	430:434	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	3	37	theme	composition	452:462	arg1	analysis					464:471	monosaccharide composition analysis	437:471	monosaccharide composition analysis	437:471	The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy.					
33142621	1	38	theme	DEAE-52	234:240	arg1	cellulose					242:250	DEAE-52 cellulose	234:250	DEAE-52 cellulose	234:250	The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100.					
33130264	0	0	theme	polysaccharide	109:122	arg1	α-glucosidase					47:59	α-glucosidase	47:59	α-glucosidase	47:59	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	0	0	theme	polysaccharide	109:122	arg1	α-amylase					65:73	α-amylase	65:73	α-amylase	65:73	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	7	1	theme	α-glucosidase	1127:1139	arg1	activity					1152:1159	α-glucosidase inhibitory activity	1127:1159	α-glucosidase inhibitory activity	1127:1159	AWPA had effectively α-glucosidase inhibitory activity and α-amylase inhibitory activity with IC50 of 0.667 mg/mL and 1.360 mg/mL, respectively.					
33130264	7	2	theme	α-amylase	1165:1173	arg1	activity					1186:1193	α-amylase inhibitory activity	1165:1193	α-amylase inhibitory activity	1165:1193	AWPA had effectively α-glucosidase inhibitory activity and α-amylase inhibitory activity with IC50 of 0.667 mg/mL and 1.360 mg/mL, respectively.					
33130264	8	3	with	reversible	1323:1332	arg1	type					1345:1348	mixed type	1339:1348	mixed type	1339:1348	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	8	3	with	reversible	1323:1332	arg1	competition					1371:1381	competitive type competition	1354:1381	competitive type competition	1354:1381	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	0	4	theme	water-soluble	95:107	arg1	polysaccharide					109:122	alkali-extracted water-soluble polysaccharide	78:122	alkali-extracted water-soluble polysaccharide from Annona squamosa residue	78:151	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	0	5	from	characterization	11:26	arg1	α-glucosidase					47:59	α-glucosidase	47:59	α-glucosidase	47:59	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	0	5	from	characterization	11:26	arg1	α-amylase					65:73	α-amylase	65:73	α-amylase	65:73	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	9	6	theme	inhibitors	1537:1546	arg1	developments					1521:1532	the developments	1517:1532	the developments of inhibitors of α-glucosidase and α-amylase	1517:1577	The significance of manuscript was not only to avoid the waste of Annona squamosa residue, but provided alternative in the developments of inhibitors of α-glucosidase and α-amylase.					
33130264	2	7	theme	homogeneous	366:376	arg1	polysaccharide					378:391	a homogeneous polysaccharide	364:391	a homogeneous polysaccharide with molecular weight of 3.08 × 103 kDa	364:431	HPLC analysis indicated that AWPA was a homogeneous polysaccharide with molecular weight of 3.08 × 103 kDa.					
33130264	2	7	theme	homogeneous	366:376	arg1	AWPA					355:358	AWPA	355:358	AWPA	355:358	HPLC analysis indicated that AWPA was a homogeneous polysaccharide with molecular weight of 3.08 × 103 kDa.					
33130264	0	8	theme	Annona	129:134	arg1	residue					145:151	Annona squamosa residue	129:151	Annona squamosa residue	129:151	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	8	9	theme	competitive	1354:1364	arg1	competition					1371:1381	competitive type competition	1354:1381	competitive type competition	1354:1381	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	4	10	theme	-β-D-Galp	832:840	arg1	1→					862:863	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	1→	862:863	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	3	11	theme	D-galacturonic	573:586	arg1	L-arabinose					525:535	L-arabinose	525:535	L-arabinose	525:535	The monosaccharide composition of AWPA, determined by ion chromatography, was consisted of L-arabinose, D-galactose, d-glucose, D-mannose, D-galacturonic acid in a percentage of 15.58:13.48:60.14:9.02:1.78, respectively.					
33130264	3	11	theme	D-galacturonic	573:586	arg1	acid					588:591	D-galacturonic acid	573:591	D-galacturonic acid	573:591	The monosaccharide composition of AWPA, determined by ion chromatography, was consisted of L-arabinose, D-galactose, d-glucose, D-mannose, D-galacturonic acid in a percentage of 15.58:13.48:60.14:9.02:1.78, respectively.					
33130264	6	12	theme	AWPA	1054:1057	arg1	reticular					1063:1071	reticular	1063:1071	reticular	1063:1071	The microstructure of AWPA was detected by scanning electron microscopy, showing that the shape of AWPA was reticular and its structure was irregular.					
33130264	6	12	theme	AWPA	1054:1057	arg1	shape					1045:1049	the shape	1041:1049	the shape of AWPA	1041:1057	The microstructure of AWPA was detected by scanning electron microscopy, showing that the shape of AWPA was reticular and its structure was irregular.					
33130264	0	13	from	α-amylase	65:73	arg1	residue					145:151	Annona squamosa residue	129:151	Annona squamosa residue	129:151	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	5	14	theme	red	890:892	arg1	experiment					894:903	The Congo red experiment	880:903	The Congo red experiment on AWPA	880:911	The Congo red experiment on AWPA showed that there was helix conformation.					
33130264	7	15	theme	inhibitory	1175:1184	arg1	activity					1186:1193	α-amylase inhibitory activity	1165:1193	α-amylase inhibitory activity	1165:1193	AWPA had effectively α-glucosidase inhibitory activity and α-amylase inhibitory activity with IC50 of 0.667 mg/mL and 1.360 mg/mL, respectively.					
33130264	4	16	theme	α-L-Araf-	759:767	arg1	1→					862:863	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	1→	862:863	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	4	17	theme	NMR	693:695	arg1	results					659:665	The results	655:665	The results of FT-IR, methylation and NMR	655:695	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	4	18	theme	-β-D-Glcp-	812:821	arg1	1→					862:863	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	1→	862:863	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	9	19	theme	manuscript	1418:1427	arg1	significance					1402:1413	The significance	1398:1413	The significance of manuscript	1398:1427	The significance of manuscript was not only to avoid the waste of Annona squamosa residue, but provided alternative in the developments of inhibitors of α-glucosidase and α-amylase.					
33130264	4	20	theme	1→	787:788	arg1	1→					862:863	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	1→	862:863	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	4	21	theme	sugar	713:717	arg1	AWPA					730:733	AWPA	730:733	AWPA	730:733	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	4	21	theme	sugar	713:717	arg1	residue					719:725	the sugar residue	709:725	the sugar residue of AWPA	709:733	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	6	22	theme	scanning	998:1005	arg1	microscopy					1016:1025	scanning electron microscopy	998:1025	scanning electron microscopy	998:1025	The microstructure of AWPA was detected by scanning electron microscopy, showing that the shape of AWPA was reticular and its structure was irregular.					
33130264	0	23	theme	squamosa	136:143	arg1	residue					145:151	Annona squamosa residue	129:151	Annona squamosa residue	129:151	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	0	24	from	α-glucosidase	47:59	arg1	residue					145:151	Annona squamosa residue	129:151	Annona squamosa residue	129:151	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	6	25	theme	electron	1007:1014	arg1	microscopy					1016:1025	scanning electron microscopy	998:1025	scanning electron microscopy	998:1025	The microstructure of AWPA was detected by scanning electron microscopy, showing that the shape of AWPA was reticular and its structure was irregular.					
33130264	2	26	theme	3.08 × 103 kDa	418:431	arg1	weight					408:413	molecular weight	398:413	molecular weight of 3.08 × 103 kDa	398:431	HPLC analysis indicated that AWPA was a homogeneous polysaccharide with molecular weight of 3.08 × 103 kDa.					
33130264	9	27	theme	Annona	1464:1469	arg1	residue					1480:1486	Annona squamosa residue	1464:1486	Annona squamosa residue	1464:1486	The significance of manuscript was not only to avoid the waste of Annona squamosa residue, but provided alternative in the developments of inhibitors of α-glucosidase and α-amylase.					
33130264	7	28	theme	inhibitory	1141:1150	arg1	activity					1152:1159	α-glucosidase inhibitory activity	1127:1159	α-glucosidase inhibitory activity	1127:1159	AWPA had effectively α-glucosidase inhibitory activity and α-amylase inhibitory activity with IC50 of 0.667 mg/mL and 1.360 mg/mL, respectively.					
33130264	4	29	theme	1→	823:824	arg1	1→					862:863	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	1→	862:863	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	8	30	from	effects	1266:1272	arg1	α-glucosidase					1285:1297	α-glucosidase	1285:1297	α-glucosidase	1285:1297	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	8	30	from	effects	1266:1272	arg1	α-amylase					1303:1311	α-amylase	1303:1311	α-amylase	1303:1311	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	3	31	theme	AWPA	468:471	arg1	composition					453:463	The monosaccharide composition	434:463	The monosaccharide composition	434:463	The monosaccharide composition of AWPA, determined by ion chromatography, was consisted of L-arabinose, D-galactose, d-glucose, D-mannose, D-galacturonic acid in a percentage of 15.58:13.48:60.14:9.02:1.78, respectively.					
33130264	0	32	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	8	33	theme	inhibitory	1255:1264	arg1	both					1318:1321	both	1318:1321	both	1318:1321	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	8	33	theme	inhibitory	1255:1264	arg1	effects					1266:1272	The inhibitory effects	1251:1272	The inhibitory effects of AWPA on α-glucosidase and α-amylase	1251:1311	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	8	33	theme	inhibitory	1255:1264	arg1	reversible					1323:1332	reversible	1323:1332	reversible	1323:1332	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	9	34	theme	α-amylase	1569:1577	arg1	inhibitors					1537:1546	inhibitors	1537:1546	inhibitors of α-glucosidase and α-amylase	1537:1577	The significance of manuscript was not only to avoid the waste of Annona squamosa residue, but provided alternative in the developments of inhibitors of α-glucosidase and α-amylase.					
33130264	2	35	with	polysaccharide	378:391	arg1	weight					408:413	molecular weight	398:413	molecular weight of 3.08 × 103 kDa	398:431	HPLC analysis indicated that AWPA was a homogeneous polysaccharide with molecular weight of 3.08 × 103 kDa.					
33130264	7	36	theme	1.360 mg/mL	1224:1234	arg1	IC50					1200:1203	IC50	1200:1203	IC50 of 0.667 mg/mL and 1.360 mg/mL	1200:1234	AWPA had effectively α-glucosidase inhibitory activity and α-amylase inhibitory activity with IC50 of 0.667 mg/mL and 1.360 mg/mL, respectively.					
33130264	9	37	from	alternative	1502:1512	arg1	developments					1521:1532	the developments	1517:1532	the developments of inhibitors of α-glucosidase and α-amylase	1517:1577	The significance of manuscript was not only to avoid the waste of Annona squamosa residue, but provided alternative in the developments of inhibitors of α-glucosidase and α-amylase.					
33130264	4	38	theme	-α-D-Manp-	851:860	arg1	1→					862:863	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	1→	862:863	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	2	39	theme	HPLC	326:329	arg1	analysis					331:338	HPLC analysis	326:338	HPLC analysis	326:338	HPLC analysis indicated that AWPA was a homogeneous polysaccharide with molecular weight of 3.08 × 103 kDa.					
33130264	4	40	theme	methylation	677:687	arg1	results					659:665	The results	655:665	The results of FT-IR, methylation and NMR	655:695	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	1	41	theme	Sephadex	310:317	arg1	G-150					319:323	Sephadex G-150	310:323	Sephadex G-150	310:323	A novel acidic polysaccharide, named as AWPA, was extracted form Annona squamosa residue by 0.1 M NaOH alkaline solution and purified by DEAE-cellulose and Sephadex G-150.					
33130264	4	42	theme	AWPA	730:733	arg1	AWPA					730:733	AWPA	730:733	AWPA	730:733	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	4	42	theme	AWPA	730:733	arg1	residue					719:725	the sugar residue	709:725	the sugar residue of AWPA	709:733	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	9	43	theme	squamosa	1471:1478	arg1	residue					1480:1486	Annona squamosa residue	1464:1486	Annona squamosa residue	1464:1486	The significance of manuscript was not only to avoid the waste of Annona squamosa residue, but provided alternative in the developments of inhibitors of α-glucosidase and α-amylase.					
33130264	1	44	theme	Annona	219:224	arg1	residue					235:241	Annona squamosa residue	219:241	Annona squamosa residue	219:241	A novel acidic polysaccharide, named as AWPA, was extracted form Annona squamosa residue by 0.1 M NaOH alkaline solution and purified by DEAE-cellulose and Sephadex G-150.					
33130264	3	45	theme	ion	488:490	arg1	chromatography					492:505	ion chromatography	488:505	ion chromatography	488:505	The monosaccharide composition of AWPA, determined by ion chromatography, was consisted of L-arabinose, D-galactose, d-glucose, D-mannose, D-galacturonic acid in a percentage of 15.58:13.48:60.14:9.02:1.78, respectively.					
33130264	8	46	theme	mixed	1339:1343	arg1	type					1345:1348	mixed type	1339:1348	mixed type	1339:1348	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	1	47	theme	squamosa	226:233	arg1	residue					235:241	Annona squamosa residue	219:241	Annona squamosa residue	219:241	A novel acidic polysaccharide, named as AWPA, was extracted form Annona squamosa residue by 0.1 M NaOH alkaline solution and purified by DEAE-cellulose and Sephadex G-150.					
33130264	8	48	dep	reversible	1323:1332	arg1	effects					1266:1272	The inhibitory effects	1251:1272	The inhibitory effects of AWPA on α-glucosidase and α-amylase	1251:1311	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	8	48	dep	reversible	1323:1332	arg1	both					1318:1321	both	1318:1321	both	1318:1321	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	8	48	dep	reversible	1323:1332	arg1	reversible					1323:1332	reversible	1323:1332	reversible	1323:1332	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	4	49	theme	FT-IR	670:674	arg1	results					659:665	The results	655:665	The results of FT-IR, methylation and NMR	655:695	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	4	50	dep	1→	862:863	arg1	→4,6					827:830	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	→4,6	827:830	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	4	50	dep	1→	862:863	arg1	→3,6					846:849	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	→3,6	846:849	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	4	50	dep	1→	862:863	arg1	→4					791:792	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	→4	791:792	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	4	51	theme	1→	842:843	arg1	1→					862:863	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	1→	862:863	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	3	52	theme	15.58:13.48:60.14:9.02:1.78	612:638	arg1	percentage					598:607	a percentage	596:607	a percentage of 15.58:13.48:60.14:9.02:1.78, respectively	596:652	The monosaccharide composition of AWPA, determined by ion chromatography, was consisted of L-arabinose, D-galactose, d-glucose, D-mannose, D-galacturonic acid in a percentage of 15.58:13.48:60.14:9.02:1.78, respectively.					
33130264	7	53	theme	0.667 mg/mL	1208:1218	arg1	IC50					1200:1203	IC50	1200:1203	IC50 of 0.667 mg/mL and 1.360 mg/mL	1200:1234	AWPA had effectively α-glucosidase inhibitory activity and α-amylase inhibitory activity with IC50 of 0.667 mg/mL and 1.360 mg/mL, respectively.					
33130264	3	54	theme	monosaccharide	438:451	arg1	composition					453:463	The monosaccharide composition	434:463	The monosaccharide composition	434:463	The monosaccharide composition of AWPA, determined by ion chromatography, was consisted of L-arabinose, D-galactose, d-glucose, D-mannose, D-galacturonic acid in a percentage of 15.58:13.48:60.14:9.02:1.78, respectively.					
33130264	0	55	from	residue	145:151	arg1	α-glucosidase					47:59	α-glucosidase	47:59	α-glucosidase	47:59	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	0	55	from	residue	145:151	arg1	polysaccharide					109:122	alkali-extracted water-soluble polysaccharide	78:122	alkali-extracted water-soluble polysaccharide from Annona squamosa residue	78:151	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	0	55	from	residue	145:151	arg1	α-amylase					65:73	α-amylase	65:73	α-amylase	65:73	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	9	56	theme	α-glucosidase	1551:1563	arg1	inhibitors					1537:1546	inhibitors	1537:1546	inhibitors of α-glucosidase and α-amylase	1537:1577	The significance of manuscript was not only to avoid the waste of Annona squamosa residue, but provided alternative in the developments of inhibitors of α-glucosidase and α-amylase.					
33130264	8	57	theme	type	1366:1369	arg1	competition					1371:1381	competitive type competition	1354:1381	competitive type competition	1354:1381	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	5	58	theme	helix	935:939	arg1	conformation					941:952	helix conformation	935:952	helix conformation	935:952	The Congo red experiment on AWPA showed that there was helix conformation.					
33130264	1	59	theme	novel	156:160	arg1	polysaccharide					169:182	A novel acidic polysaccharide	154:182	A novel acidic polysaccharide	154:182	A novel acidic polysaccharide, named as AWPA, was extracted form Annona squamosa residue by 0.1 M NaOH alkaline solution and purified by DEAE-cellulose and Sephadex G-150.					
33130264	7	60	contain	had	1111:1113	arg2	activity					1152:1159	α-glucosidase inhibitory activity	1127:1159	α-glucosidase inhibitory activity	1127:1159	AWPA had effectively α-glucosidase inhibitory activity and α-amylase inhibitory activity with IC50 of 0.667 mg/mL and 1.360 mg/mL, respectively.					
33130264	7	60	contain	had	1111:1113	arg1	AWPA					1106:1109	AWPA	1106:1109	AWPA	1106:1109	AWPA had effectively α-glucosidase inhibitory activity and α-amylase inhibitory activity with IC50 of 0.667 mg/mL and 1.360 mg/mL, respectively.					
33130264	7	60	contain	had	1111:1113	arg2	activity					1186:1193	α-amylase inhibitory activity	1165:1193	α-amylase inhibitory activity	1165:1193	AWPA had effectively α-glucosidase inhibitory activity and α-amylase inhibitory activity with IC50 of 0.667 mg/mL and 1.360 mg/mL, respectively.					
33130264	9	61	theme	residue	1480:1486	arg1	waste					1455:1459	the waste	1451:1459	the waste of Annona squamosa residue	1451:1486	The significance of manuscript was not only to avoid the waste of Annona squamosa residue, but provided alternative in the developments of inhibitors of α-glucosidase and α-amylase.					
33130264	4	62	theme	-α-D-Glcp-	776:785	arg1	1→					862:863	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	1→	862:863	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	1	63	theme	acidic	162:167	arg1	polysaccharide					169:182	A novel acidic polysaccharide	154:182	A novel acidic polysaccharide	154:182	A novel acidic polysaccharide, named as AWPA, was extracted form Annona squamosa residue by 0.1 M NaOH alkaline solution and purified by DEAE-cellulose and Sephadex G-150.					
33130264	8	64	theme	AWPA	1277:1280	arg1	both					1318:1321	both	1318:1321	both	1318:1321	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	8	64	theme	AWPA	1277:1280	arg1	effects					1266:1272	The inhibitory effects	1251:1272	The inhibitory effects of AWPA on α-glucosidase and α-amylase	1251:1311	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	8	64	theme	AWPA	1277:1280	arg1	reversible					1323:1332	reversible	1323:1332	reversible	1323:1332	The inhibitory effects of AWPA on α-glucosidase and α-amylase were both reversible with mixed type and competitive type competition, respectively.					
33130264	1	65	theme	0.1 M	246:250	arg1	solution					266:273	0.1 M NaOH alkaline solution	246:273	0.1 M NaOH alkaline solution	246:273	A novel acidic polysaccharide, named as AWPA, was extracted form Annona squamosa residue by 0.1 M NaOH alkaline solution and purified by DEAE-cellulose and Sephadex G-150.					
33130264	0	66	theme	alkali-extracted	78:93	arg1	polysaccharide					109:122	alkali-extracted water-soluble polysaccharide	78:122	alkali-extracted water-soluble polysaccharide from Annona squamosa residue	78:151	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	4	67	theme	-β-D-Galp-	794:803	arg1	1→					862:863	α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→	759:863	1→	862:863	The results of FT-IR, methylation and NMR showed that the sugar residue of AWPA were mainly composed of α-L-Araf-(1→, →4)-α-D-Glcp-(1→, →4)-β-D-Galp-(1→, →6)-β-D-Glcp-(1→, →4,6)-β-D-Galp(1→, →3,6)-α-D-Manp-(1→, respectively.					
33130264	6	68	theme	AWPA	977:980	arg1	microstructure					959:972	The microstructure	955:972	The microstructure of AWPA	955:980	The microstructure of AWPA was detected by scanning electron microscopy, showing that the shape of AWPA was reticular and its structure was irregular.					
33130264	2	69	theme	molecular	398:406	arg1	weight					408:413	molecular weight	398:413	molecular weight of 3.08 × 103 kDa	398:431	HPLC analysis indicated that AWPA was a homogeneous polysaccharide with molecular weight of 3.08 × 103 kDa.					
33130264	1	70	theme	NaOH	252:255	arg1	solution					266:273	0.1 M NaOH alkaline solution	246:273	0.1 M NaOH alkaline solution	246:273	A novel acidic polysaccharide, named as AWPA, was extracted form Annona squamosa residue by 0.1 M NaOH alkaline solution and purified by DEAE-cellulose and Sephadex G-150.					
33130264	0	71	from	inhibitions	32:42	arg1	α-glucosidase					47:59	α-glucosidase	47:59	α-glucosidase	47:59	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	0	71	from	inhibitions	32:42	arg1	α-amylase					65:73	α-amylase	65:73	α-amylase	65:73	Structural characterization and inhibitions on α-glucosidase and α-amylase of alkali-extracted water-soluble polysaccharide from Annona squamosa residue.					
33130264	5	72	from	experiment	894:903	arg1	AWPA					908:911	AWPA	908:911	AWPA	908:911	The Congo red experiment on AWPA showed that there was helix conformation.					
33130264	1	73	theme	alkaline	257:264	arg1	solution					266:273	0.1 M NaOH alkaline solution	246:273	0.1 M NaOH alkaline solution	246:273	A novel acidic polysaccharide, named as AWPA, was extracted form Annona squamosa residue by 0.1 M NaOH alkaline solution and purified by DEAE-cellulose and Sephadex G-150.					
33429349	7	0	theme	crucial	1319:1325	arg1	insights					1327:1334	The crucial insights	1315:1334	The crucial insights from this study	1315:1350	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	7	1	dep	fields	1517:1522	arg1	parameters					1535:1544	processing parameters	1524:1544	processing parameters	1524:1544	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	7	1	dep	fields	1517:1522	arg1	properties					1554:1563	the properties	1550:1563	the properties of the colloidal systems such as the pH	1550:1603	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	1	2	theme	high	176:179	arg1	incompatibility					181:195	The high incompatibility	172:195	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides	161:254	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides require a series of modifications to develop stable microstructures effectively.					
33429349	1	3	theme	modifications	276:288	arg1	series					266:271	a series	264:271	a series of modifications	264:288	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides require a series of modifications to develop stable microstructures effectively.					
33429349	4	4	theme	390-410 kJ/kg	977:989	arg1	energy					967:972	a specific energy	956:972	a specific energy of 390-410 kJ/kg	956:989	FINDINGS Superior complex coacervated matrices were assembled at pH 4.0 and 5.7 before and after pulsed electric fields treatment at a specific energy of 390-410 kJ/kg.					
33429349	3	5	theme	dispersion	770:779	arg1	self-assembly					689:701	self-assembly	689:701	self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	689:779	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	4	6	theme	electric	927:934	arg1	fields					936:941	pulsed electric fields	920:941	pulsed electric fields	920:941	FINDINGS Superior complex coacervated matrices were assembled at pH 4.0 and 5.7 before and after pulsed electric fields treatment at a specific energy of 390-410 kJ/kg.					
33429349	6	7	theme	electric	1184:1191	arg1	intensity					1199:1207	very high electric field intensity	1174:1207	very high electric field intensity	1174:1207	Irrespective of the pH, very high electric field intensity caused excessive system perturbation leading to severe fragmentation and poor development of coacervates.					
33429349	5	8	theme	pulsed	1049:1054	arg1	alteration					1080:1089	pulsed electric fields-induced alteration	1049:1089	pulsed electric fields-induced alteration of functional group interactions	1049:1122	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	4	9	theme	coacervated	849:859	arg1	matrices					861:868	FINDINGS Superior complex coacervated matrices	823:868	FINDINGS Superior complex coacervated matrices	823:868	FINDINGS Superior complex coacervated matrices were assembled at pH 4.0 and 5.7 before and after pulsed electric fields treatment at a specific energy of 390-410 kJ/kg.					
33429349	5	10	theme	electric	1056:1063	arg1	alteration					1080:1089	pulsed electric fields-induced alteration	1049:1089	pulsed electric fields-induced alteration of functional group interactions	1049:1122	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	5	11	theme	pH-dependent	1129:1140	arg1	manner					1142:1147	a pH-dependent manner	1127:1147	a pH-dependent manner	1127:1147	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	3	12	from	impact	578:583	arg1	energy					625:630	a specific energy	614:630	a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm)	614:684	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	3	12	from	impact	578:583	arg1	self-assembly					689:701	self-assembly	689:701	self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	689:779	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	0	13	dep	-polyethylene	113:125	arg1	alcohol					105:111	vinyl alcohol	99:111	vinyl alcohol	99:111	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	0	14	theme	vinyl	99:103	arg1	alcohol					105:111	vinyl alcohol	99:111	vinyl alcohol	99:111	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	4	15	theme	FINDINGS	823:830	arg1	matrices					861:868	FINDINGS Superior complex coacervated matrices	823:868	FINDINGS Superior complex coacervated matrices	823:868	FINDINGS Superior complex coacervated matrices were assembled at pH 4.0 and 5.7 before and after pulsed electric fields treatment at a specific energy of 390-410 kJ/kg.					
33429349	2	16	theme	pulsed	394:399	arg1	fields					410:415	pulsed electric fields	394:415	pulsed electric fields processing	394:426	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	1	17	theme	stable	301:306	arg1	microstructures					308:322	stable microstructures	301:322	stable microstructures	301:322	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides require a series of modifications to develop stable microstructures effectively.					
33429349	0	18	theme	-polyethylene	113:125	arg1	composites					149:158	chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	80:158	chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	80:158	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	6	19	theme	system	1226:1231	arg1	perturbation					1233:1244	excessive system perturbation	1216:1244	excessive system perturbation leading to severe fragmentation and poor development of coacervates	1216:1312	Irrespective of the pH, very high electric field intensity caused excessive system perturbation leading to severe fragmentation and poor development of coacervates.					
33429349	5	20	theme	homogenous	1008:1017	arg1	behaviour					1019:1027	The compact and homogenous behaviour	992:1027	The compact and homogenous behaviour	992:1027	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	5	20	theme	homogenous	1008:1017	arg1	attributable					1033:1044	attributable	1033:1044	attributable	1033:1044	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	6	21	theme	high	1179:1182	arg1	intensity					1199:1207	very high electric field intensity	1174:1207	very high electric field intensity	1174:1207	Irrespective of the pH, very high electric field intensity caused excessive system perturbation leading to severe fragmentation and poor development of coacervates.					
33429349	3	22	theme	electric	595:602	arg1	fields					604:609	pulsed electric fields	588:609	pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm)	588:684	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	7	23	theme	different	1447:1455	arg1	charges					1463:1469	different local charges	1447:1469	different local charges	1447:1469	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	2	24	theme	inter/intramolecular	440:459	arg1	interactions					461:472	inter/intramolecular interactions	440:472	inter/intramolecular interactions	440:472	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	2	25	theme	nanostructured	522:535	arg1	composites					537:546	bio-based nanostructured composites	512:546	bio-based nanostructured composites	512:546	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	3	26	theme	fields	604:609	arg1	impact					578:583	the impact	574:583	the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	574:779	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	1	27	theme	bio-based	200:208	arg1	materials					210:218	bio-based materials	200:218	bio-based materials such as protein and polysaccharides	200:254	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides require a series of modifications to develop stable microstructures effectively.					
33429349	1	27	theme	bio-based	200:208	arg1	protein					228:234	protein	228:234	protein	228:234	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides require a series of modifications to develop stable microstructures effectively.					
33429349	1	27	theme	bio-based	200:208	arg1	polysaccharides					240:254	polysaccharides	240:254	polysaccharides	240:254	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides require a series of modifications to develop stable microstructures effectively.					
33429349	2	28	theme	bio-based	512:520	arg1	composites					537:546	bio-based nanostructured composites	512:546	bio-based nanostructured composites	512:546	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	3	29	theme	pulsed	588:593	arg1	fields					604:609	pulsed electric fields	588:609	pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm)	588:684	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	4	30	theme	pulsed	920:925	arg1	fields					936:941	pulsed electric fields	920:941	pulsed electric fields	920:941	FINDINGS Superior complex coacervated matrices were assembled at pH 4.0 and 5.7 before and after pulsed electric fields treatment at a specific energy of 390-410 kJ/kg.					
33429349	6	31	theme	severe	1257:1262	arg1	fragmentation					1264:1276	severe fragmentation	1257:1276	severe fragmentation	1257:1276	Irrespective of the pH, very high electric field intensity caused excessive system perturbation leading to severe fragmentation and poor development of coacervates.					
33429349	1	32	theme	materials	210:218	arg1	HYPOTHESIS					161:170	HYPOTHESIS	161:170	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides	161:254	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides require a series of modifications to develop stable microstructures effectively.					
33429349	7	33	theme	colloidal	1572:1580	arg1	pH					1602:1603	the pH	1598:1603	the pH	1598:1603	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	7	33	theme	colloidal	1572:1580	arg1	systems					1582:1588	the colloidal systems	1568:1588	the colloidal systems such as the pH	1568:1603	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	3	34	theme	60-700 kJ/kg	635:646	arg1	energy					625:630	a specific energy	614:630	a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm)	614:684	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	7	35	theme	local	1457:1461	arg1	charges					1463:1469	different local charges	1447:1469	different local charges	1447:1469	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	7	36	theme	pulsed	1501:1506	arg1	fields					1517:1522	pulsed electric fields	1501:1522	pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH	1501:1603	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	7	37	from	study	1346:1350	arg1	insights					1327:1334	The crucial insights	1315:1334	The crucial insights from this study	1315:1350	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	0	38	theme	nanostructured	134:147	arg1	composites					149:158	chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	80:158	chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	80:158	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	5	39	theme	compact	996:1002	arg1	behaviour					1019:1027	The compact and homogenous behaviour	992:1027	The compact and homogenous behaviour	992:1027	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	5	39	theme	compact	996:1002	arg1	attributable					1033:1044	attributable	1033:1044	attributable	1033:1044	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	1	40	dep	HYPOTHESIS	161:170	arg1	incompatibility					181:195	The high incompatibility	172:195	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides	161:254	HYPOTHESIS The high incompatibility of bio-based materials such as protein and polysaccharides require a series of modifications to develop stable microstructures effectively.					
33429349	5	41	theme	group	1105:1109	arg1	interactions					1111:1122	functional group interactions	1094:1122	functional group interactions	1094:1122	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	7	42	theme	systems	1428:1434	arg1	behaviour					1382:1390	the self-assembly behaviour	1364:1390	the self-assembly behaviour	1364:1390	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	7	42	theme	systems	1428:1434	arg1	integration					1396:1406	integration	1396:1406	integration	1396:1406	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	0	43	theme	pH	16:17	arg1	influence					19:27	the pH influence	12:27	the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	12:158	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	0	44	theme	glycol	127:132	arg1	composites					149:158	chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	80:158	chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	80:158	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	3	45	theme	vinyl	725:729	arg1	alcohol					731:737	vinyl alcohol	725:737	vinyl alcohol	725:737	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	6	46	theme	field	1193:1197	arg1	intensity					1199:1207	very high electric field intensity	1174:1207	very high electric field intensity	1174:1207	Irrespective of the pH, very high electric field intensity caused excessive system perturbation leading to severe fragmentation and poor development of coacervates.					
33429349	0	47	theme	pulsed	32:37	arg1	self-assembly					63:75	pulsed electric fields-induced self-assembly	32:75	pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	32:158	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	6	48	theme	excessive	1216:1224	arg1	perturbation					1233:1244	excessive system perturbation	1216:1244	excessive system perturbation leading to severe fragmentation and poor development of coacervates	1216:1312	Irrespective of the pH, very high electric field intensity caused excessive system perturbation leading to severe fragmentation and poor development of coacervates.					
33429349	5	49	from	attributable	1033:1044	arg1	manner					1142:1147	a pH-dependent manner	1127:1147	a pH-dependent manner	1127:1147	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	3	50	theme	field	658:662	arg1	strength = 1.6 kV/cm					664:683	electric field strength = 1.6 kV/cm	649:683	electric field strength = 1.6 kV/cm	649:683	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	3	50	theme	field	658:662	arg1	60-700 kJ/kg					635:646	60-700 kJ/kg	635:646	60-700 kJ/kg (electric field strength = 1.6 kV/cm)	635:684	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	5	51	from	manner	1142:1147	arg1	behaviour					1019:1027	The compact and homogenous behaviour	992:1027	The compact and homogenous behaviour	992:1027	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	5	51	from	manner	1142:1147	arg1	attributable					1033:1044	attributable	1033:1044	attributable	1033:1044	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	7	52	theme	systems	1582:1588	arg1	parameters					1535:1544	processing parameters	1524:1544	processing parameters	1524:1544	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	7	52	theme	systems	1582:1588	arg1	properties					1554:1563	the properties	1550:1563	the properties of the colloidal systems such as the pH	1550:1603	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	0	53	theme	composites	149:158	arg1	self-assembly					63:75	pulsed electric fields-induced self-assembly	32:75	pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	32:158	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	3	54	theme	zein-chitosan-poly	706:723	arg1	dispersion					770:779	zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	706:779	zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	706:779	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	5	55	theme	interactions	1111:1122	arg1	alteration					1080:1089	pulsed electric fields-induced alteration	1049:1089	pulsed electric fields-induced alteration of functional group interactions	1049:1122	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	7	56	theme	electric	1508:1515	arg1	fields					1517:1522	pulsed electric fields	1501:1522	pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH	1501:1603	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	7	57	theme	biopolymer-based	1411:1426	arg1	systems					1428:1434	biopolymer-based systems	1411:1434	biopolymer-based systems possessing different local charges	1411:1469	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	2	58	theme	composites	537:546	arg1	compatibility					475:487	compatibility	475:487	compatibility	475:487	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	2	58	theme	composites	537:546	arg1	microstructure					494:507	microstructure	494:507	microstructure of bio-based nanostructured composites	494:546	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	2	58	theme	composites	537:546	arg1	interactions					461:472	inter/intramolecular interactions	440:472	inter/intramolecular interactions	440:472	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	4	59	theme	specific	958:965	arg1	energy					967:972	a specific energy	956:972	a specific energy of 390-410 kJ/kg	956:989	FINDINGS Superior complex coacervated matrices were assembled at pH 4.0 and 5.7 before and after pulsed electric fields treatment at a specific energy of 390-410 kJ/kg.					
33429349	4	60	from	energy	967:972	arg1	fields					936:941	pulsed electric fields	920:941	pulsed electric fields	920:941	FINDINGS Superior complex coacervated matrices were assembled at pH 4.0 and 5.7 before and after pulsed electric fields treatment at a specific energy of 390-410 kJ/kg.					
33429349	4	60	from	energy	967:972	arg1	treatment					943:951	treatment	943:951	treatment at a specific energy of 390-410 kJ/kg	943:989	FINDINGS Superior complex coacervated matrices were assembled at pH 4.0 and 5.7 before and after pulsed electric fields treatment at a specific energy of 390-410 kJ/kg.					
33429349	7	61	contain	possessing	1436:1445	arg1	systems					1428:1434	biopolymer-based systems	1411:1434	biopolymer-based systems possessing different local charges	1411:1469	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	7	61	contain	possessing	1436:1445	arg2	charges					1463:1469	different local charges	1447:1469	different local charges	1447:1469	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	2	62	theme	residues	384:391	arg1	charge					366:371	charge	366:371	charge	366:371	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	2	62	theme	residues	384:391	arg1	density					354:360	density	354:360	density	354:360	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	0	63	theme	electric	39:46	arg1	self-assembly					63:75	pulsed electric fields-induced self-assembly	32:75	pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	32:158	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	6	64	theme	poor	1282:1285	arg1	development					1287:1297	poor development	1282:1297	poor development	1282:1297	Irrespective of the pH, very high electric field intensity caused excessive system perturbation leading to severe fragmentation and poor development of coacervates.					
33429349	3	65	theme	glycol	753:758	arg1	dispersion					770:779	zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	706:779	zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	706:779	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	3	66	theme	electric	649:656	arg1	strength = 1.6 kV/cm					664:683	electric field strength = 1.6 kV/cm	649:683	electric field strength = 1.6 kV/cm	649:683	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	3	66	theme	electric	649:656	arg1	60-700 kJ/kg					635:646	60-700 kJ/kg	635:646	60-700 kJ/kg (electric field strength = 1.6 kV/cm)	635:684	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	2	67	theme	surface	376:382	arg1	residues					384:391	surface residues	376:391	surface residues	376:391	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	3	68	theme	composite	760:768	arg1	dispersion					770:779	zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	706:779	zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	706:779	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	5	69	theme	fields-induced	1065:1078	arg1	alteration					1080:1089	pulsed electric fields-induced alteration	1049:1089	pulsed electric fields-induced alteration of functional group interactions	1049:1122	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	7	70	theme	processing	1524:1533	arg1	parameters					1535:1544	processing parameters	1524:1544	processing parameters	1524:1544	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	7	71	theme	self-assembly	1368:1380	arg1	behaviour					1382:1390	the self-assembly behaviour	1364:1390	the self-assembly behaviour	1364:1390	The crucial insights from this study reveal that the self-assembly behaviour and integration of biopolymer-based systems possessing different local charges can be enhanced by optimising pulsed electric fields processing parameters and the properties of the colloidal systems such as the pH.					
33429349	3	72	theme	specific	616:623	arg1	energy					625:630	a specific energy	614:630	a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm)	614:684	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	3	73	dep	zein-chitosan-poly	706:723	arg1	alcohol					731:737	vinyl alcohol	725:737	vinyl alcohol	725:737	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	4	74	theme	complex	841:847	arg1	matrices					861:868	FINDINGS Superior complex coacervated matrices	823:868	FINDINGS Superior complex coacervated matrices	823:868	FINDINGS Superior complex coacervated matrices were assembled at pH 4.0 and 5.7 before and after pulsed electric fields treatment at a specific energy of 390-410 kJ/kg.					
33429349	4	75	theme	Superior	832:839	arg1	matrices					861:868	FINDINGS Superior complex coacervated matrices	823:868	FINDINGS Superior complex coacervated matrices	823:868	FINDINGS Superior complex coacervated matrices were assembled at pH 4.0 and 5.7 before and after pulsed electric fields treatment at a specific energy of 390-410 kJ/kg.					
33429349	2	76	dep	density	354:360	arg1	the					350:352	the	350:352	the	350:352	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	3	77	theme	-polyethylene	739:751	arg1	dispersion					770:779	zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	706:779	zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	706:779	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	6	78	theme	coacervates	1302:1312	arg1	fragmentation					1264:1276	severe fragmentation	1257:1276	severe fragmentation	1257:1276	Irrespective of the pH, very high electric field intensity caused excessive system perturbation leading to severe fragmentation and poor development of coacervates.					
33429349	6	78	theme	coacervates	1302:1312	arg1	development					1287:1297	poor development	1282:1297	poor development	1282:1297	Irrespective of the pH, very high electric field intensity caused excessive system perturbation leading to severe fragmentation and poor development of coacervates.					
33429349	2	79	theme	fields	410:415	arg1	processing					417:426	pulsed electric fields processing	394:426	pulsed electric fields processing	394:426	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
33429349	3	80	from	energy	625:630	arg1	impact					578:583	the impact	574:583	the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion	574:779	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	3	80	from	energy	625:630	arg1	fields					604:609	pulsed electric fields	588:609	pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm)	588:684	EXPERIMENT In this work, the impact of pulsed electric fields at a specific energy of 60-700 kJ/kg (electric field strength = 1.6 kV/cm) on self-assembly of zein-chitosan-poly(vinyl alcohol)-polyethylene glycol composite dispersion was investigated at pH 4.0, 5.7, and 6.8.					
33429349	0	81	theme	fields-induced	48:61	arg1	self-assembly					63:75	pulsed electric fields-induced self-assembly	32:75	pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	32:158	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	0	82	from	influence	19:27	arg1	self-assembly					63:75	pulsed electric fields-induced self-assembly	32:75	pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites	32:158	Elucidating the pH influence on pulsed electric fields-induced self-assembly of chitosan-zein-poly(vinyl alcohol)-polyethylene glycol nanostructured composites.					
33429349	5	83	theme	functional	1094:1103	arg1	interactions					1111:1122	functional group interactions	1094:1122	functional group interactions	1094:1122	The compact and homogenous behaviour was attributable to pulsed electric fields-induced alteration of functional group interactions in a pH-dependent manner.					
33429349	2	84	theme	electric	401:408	arg1	fields					410:415	pulsed electric fields	394:415	pulsed electric fields processing	394:426	By modifying the density and charge of surface residues, pulsed electric fields processing can improve inter/intramolecular interactions, compatibility, and microstructure of bio-based nanostructured composites.					
34122956	4	0	theme	time	1104:1107	arg1	evidence					1109:1116	the first time evidence	1094:1116	the first time evidence	1094:1116	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	3	1	theme	homogeneous	772:782	arg1	glycopeptides					799:811	homogeneous synthetic MUC1 glycopeptides	772:811	homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis	772:964	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	4	2	theme	X-ray	971:975	arg1	structure					985:993	The X-ray crystal structure	967:993	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide	967:1079	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	5	3	with	complex	1354:1360	arg1	SN-101					1367:1372	SN-101	1367:1372	SN-101	1367:1372	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	1	4	theme	most	263:266	arg1	adenocarcinomas					268:282	most adenocarcinomas	263:282	most adenocarcinomas	263:282	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	4	5	theme	antibody	1023:1030	arg1	structure					985:993	The X-ray crystal structure	967:993	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide	967:1079	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	6	6	theme	disease-specific	1655:1670	arg1	O-glycosylation					1672:1686	disease-specific O-glycosylation	1655:1686	disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins	1655:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	1	7	theme	epithelial	189:198	arg1	cells					207:211	epithelial cancer cells	189:211	epithelial cancer cells	189:211	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	4	8	theme	hydrogen	1196:1203	arg1	bonds					1205:1209	multiple hydrogen bonds	1187:1209	multiple hydrogen bonds	1187:1209	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	1	9	theme	adenocarcinomas	268:282	arg1	feature					252:258	a common feature	243:258	a common feature of most adenocarcinomas	243:282	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	4	10	theme	antigenic	1058:1066	arg1	glycopeptide					1068:1079	the antigenic glycopeptide	1054:1079	the antigenic glycopeptide	1054:1079	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	6	11	dep	domains	1716:1722	arg1	the					1691:1693	the	1691:1693	the	1691:1693	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	6	12	from	O-glycosylation	1672:1686	arg1	domains					1716:1722	immunodominant mucin domains	1695:1722	immunodominant mucin domains	1695:1722	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	6	12	from	O-glycosylation	1672:1686	arg1	sequences					1739:1747	mucin-like sequences	1728:1747	mucin-like sequences	1728:1747	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	4	13	theme	multiple	1187:1194	arg1	bonds					1205:1209	multiple hydrogen bonds	1187:1209	multiple hydrogen bonds	1187:1209	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	4	14	theme	first	1098:1102	arg1	evidence					1109:1116	the first time evidence	1094:1116	the first time evidence	1094:1116	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	4	15	theme	crystal	977:983	arg1	structure					985:993	The X-ray crystal structure	967:993	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide	967:1079	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	6	16	theme	mucin	1710:1714	arg1	domains					1716:1722	immunodominant mucin domains	1695:1722	immunodominant mucin domains	1695:1722	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	5	17	theme	glycopeptide	1338:1349	arg1	identical					1377:1385	identical	1377:1385	identical	1377:1385	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	5	17	theme	glycopeptide	1338:1349	arg1	structure					1316:1324	the structure	1312:1324	the structure of the MUC1 glycopeptide in complex with SN-101	1312:1372	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	5	18	theme	site-specific	1454:1466	arg1	glycosylation					1468:1480	site-specific glycosylation	1454:1480	site-specific glycosylation	1454:1480	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	1	19	theme	cancer	200:205	arg1	cells					207:211	epithelial cancer cells	189:211	epithelial cancer cells	189:211	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	1	20	gly	O-glycosylation	126:140	arg1	proteins					145:152	proteins	145:152	proteins	145:152	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	1	20	gly	O-glycosylation	126:140	arg2	types					180:184	essentially all types	164:184	essentially all types	164:184	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	3	21	theme	synthetic	784:792	arg1	glycopeptides					799:811	homogeneous synthetic MUC1 glycopeptides	772:811	homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis	772:964	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	22	theme	epitope	925:931	arg1	process					842:848	the streamlined process	826:848	the streamlined process of immunization	826:864	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	22	theme	epitope	925:931	arg1	mapping					933:939	epitope mapping	925:939	epitope mapping	925:939	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	2	23	contain	having	487:492	arg2	O-glycans					519:527	cancer-relevant immature O-glycans	494:527	cancer-relevant immature O-glycans	494:527	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	2	23	contain	having	487:492	arg1	mucins					480:485	the most studied mucins	463:485	the most studied mucins having cancer-relevant immature O-glycans	463:527	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	3	24	theme	structure	905:913	arg1	process					842:848	the streamlined process	826:848	the streamlined process of immunization	826:864	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	24	theme	structure	905:913	arg1	analysis					915:922	three-dimensional structure analysis	887:922	three-dimensional structure analysis	887:922	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	4	25	theme	threonine	1267:1275	arg1	residue					1277:1283	the threonine residue	1263:1283	the threonine residue	1263:1283	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	1	26	theme	cells	207:211	arg1	types					180:184	essentially all types	164:184	essentially all types	164:184	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	0	27	theme	straightforward	2:16	arg1	approach					18:25	A straightforward approach	0:25	A straightforward approach to antibodies	0:39	A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes.					
34122956	3	28	theme	MUC1	794:797	arg1	glycopeptides					799:811	homogeneous synthetic MUC1 glycopeptides	772:811	homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis	772:964	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	6	29	theme	many	1794:1797	arg1	proteins					1799:1806	many proteins	1794:1806	many proteins	1794:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	1	30	theme	truncated	107:115	arg1	O-glycosylation					126:140	Aberrantly truncated immature O-glycosylation	96:140	Aberrantly truncated immature O-glycosylation in proteins	96:152	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	2	31	theme	anticancer	418:427	arg1	antibodies					429:438	anticancer antibodies	418:438	anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans	418:527	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	2	32	theme	studied	472:478	arg1	mucins					480:485	the most studied mucins	463:485	the most studied mucins having cancer-relevant immature O-glycans	463:527	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	3	33	theme	streamlined	830:840	arg1	analysis					957:964	biochemical analysis	945:964	biochemical analysis	945:964	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	33	theme	streamlined	830:840	arg1	screening					876:884	antibody screening	867:884	antibody screening	867:884	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	33	theme	streamlined	830:840	arg1	process					842:848	the streamlined process	826:848	the streamlined process of immunization	826:864	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	33	theme	streamlined	830:840	arg1	analysis					915:922	three-dimensional structure analysis	887:922	three-dimensional structure analysis	887:922	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	33	theme	streamlined	830:840	arg1	mapping					933:939	epitope mapping	925:939	epitope mapping	925:939	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	6	34	theme	neoepitopes	1628:1638	arg1	variety					1608:1614	a variety	1606:1614	a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins	1606:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	6	34	theme	neoepitopes	1628:1638	arg1	neoepitopes					1628:1638	"dynamic neoepitopes	1619:1638	"dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins	1619:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	5	35	gly	glycopeptide	1338:1349	arg2	glycopeptide					1338:1349	the MUC1 glycopeptide	1329:1349	the MUC1 glycopeptide	1329:1349	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	1	36	theme	immature	117:124	arg1	O-glycosylation					126:140	Aberrantly truncated immature O-glycosylation	96:140	Aberrantly truncated immature O-glycosylation in proteins	96:152	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	6	37	gly	O-glycosylation	1672:1686	arg1	domains					1716:1722	immunodominant mucin domains	1695:1722	immunodominant mucin domains	1695:1722	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	6	37	gly	O-glycosylation	1672:1686	arg1	sequences					1739:1747	mucin-like sequences	1728:1747	mucin-like sequences	1728:1747	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	3	38	gly	glycopeptides	799:811	arg2	glycopeptides					799:811	homogeneous synthetic MUC1 glycopeptides	772:811	homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis	772:964	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	4	39	attach	linked	1253:1258	arg1	residue					1277:1283	the threonine residue	1263:1283	the threonine residue	1263:1283	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	4	39	attach	linked	1253:1258	arg2	peptide					1234:1240	the proximal peptide	1221:1240	the proximal peptide	1221:1240	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	6	40	theme	proteins	1799:1806	arg1	regions					1783:1789	intrinsically disordered regions	1758:1789	intrinsically disordered regions of many proteins	1758:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	4	41	gly	glycopeptide	1068:1079	arg2	glycopeptide					1068:1079	the antigenic glycopeptide	1054:1079	the antigenic glycopeptide	1054:1079	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	4	42	theme	proximal	1225:1232	arg1	peptide					1234:1240	the proximal peptide	1221:1240	the proximal peptide	1221:1240	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	4	43	theme	essential	1158:1166	arg1	epitope					1168:1174	specifically the essential epitope	1141:1174	specifically the essential epitope	1141:1174	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	6	44	located	found	1749:1753	arg2	domains					1716:1722	immunodominant mucin domains	1695:1722	immunodominant mucin domains	1695:1722	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	6	44	located	found	1749:1753	arg1	regions					1783:1789	intrinsically disordered regions	1758:1789	intrinsically disordered regions of many proteins	1758:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	2	45	theme	peptide	599:605	arg1	region					607:612	the proximal MUC1 peptide region	581:612	the proximal MUC1 peptide region	581:612	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	2	46	theme	cancer-relevant	494:508	arg1	O-glycans					519:527	cancer-relevant immature O-glycans	494:527	cancer-relevant immature O-glycans	494:527	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	6	47	theme	new	1561:1563	arg1	class					1565:1569	a new class	1559:1569	a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins	1559:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	6	48	theme	immunodominant	1695:1708	arg1	domains					1716:1722	immunodominant mucin domains	1695:1722	immunodominant mucin domains	1695:1722	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	2	49	theme	MUC1	594:597	arg1	region					607:612	the proximal MUC1 peptide region	581:612	the proximal MUC1 peptide region	581:612	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	3	50	theme	antibodies	696:705	arg1	creation					684:691	the creation	680:691	the creation of antibodies interacting specifically with glycopeptidic neoepitopes	680:761	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	6	51	theme	antibodies	1585:1594	arg1	class					1565:1569	a new class	1559:1569	a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins	1559:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	3	52	theme	biochemical	945:955	arg1	analysis					957:964	biochemical analysis	945:964	biochemical analysis	945:964	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	52	theme	biochemical	945:955	arg1	process					842:848	the streamlined process	826:848	the streamlined process of immunization	826:864	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	2	53	theme	immature	510:517	arg1	O-glycans					519:527	cancer-relevant immature O-glycans	494:527	cancer-relevant immature O-glycans	494:527	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	2	54	theme	proximal	585:592	arg1	region					607:612	the proximal MUC1 peptide region	581:612	the proximal MUC1 peptide region	581:612	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	3	55	theme	general	647:653	arg1	strategy					655:662	a general strategy	645:662	a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis	645:964	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	6	56	theme	disordered	1772:1781	arg1	regions					1783:1789	intrinsically disordered regions	1758:1789	intrinsically disordered regions of many proteins	1758:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	0	57	theme	specific	60:67	arg1	neoepitopes					83:93	cancer specific glycopeptidic neoepitopes	53:93	cancer specific glycopeptidic neoepitopes	53:93	A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes.					
34122956	3	58	theme	antibody	867:874	arg1	screening					876:884	antibody screening	867:884	antibody screening	867:884	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	58	theme	antibody	867:874	arg1	process					842:848	the streamlined process	826:848	the streamlined process of immunization	826:864	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	0	59	theme	glycopeptidic	69:81	arg1	neoepitopes					83:93	cancer specific glycopeptidic neoepitopes	53:93	cancer specific glycopeptidic neoepitopes	53:93	A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes.					
34122956	6	60	theme	designated	1574:1583	arg1	antibodies					1585:1594	designated antibodies	1574:1594	designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins	1574:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	0	61	theme	cancer	53:58	arg1	neoepitopes					83:93	cancer specific glycopeptidic neoepitopes	53:93	cancer specific glycopeptidic neoepitopes	53:93	A straightforward approach to antibodies recognising cancer specific glycopeptidic neoepitopes.					
34122956	5	62	theme	solution	1394:1401	arg1	conformation					1430:1441	an extended conformation	1418:1441	an extended conformation induced by site-specific glycosylation	1418:1480	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	5	62	theme	solution	1394:1401	arg1	structure					1407:1415	its solution NMR structure	1390:1415	its solution NMR structure	1390:1415	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	5	63	from	structure	1316:1324	arg1	complex					1354:1360	complex	1354:1360	complex with SN-101	1354:1372	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	4	64	dep	peptide	1234:1240	arg1	both					1211:1214	both	1211:1214	both	1211:1214	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	5	65	theme	extended	1421:1428	arg1	conformation					1430:1441	an extended conformation	1418:1441	an extended conformation induced by site-specific glycosylation	1418:1480	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	5	65	theme	extended	1421:1428	arg1	structure					1407:1415	its solution NMR structure	1390:1415	its solution NMR structure	1390:1415	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	1	66	from	O-glycosylation	126:140	arg1	proteins					145:152	proteins	145:152	proteins	145:152	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	3	67	theme	immunization	853:864	arg1	analysis					957:964	biochemical analysis	945:964	biochemical analysis	945:964	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	67	theme	immunization	853:864	arg1	screening					876:884	antibody screening	867:884	antibody screening	867:884	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	67	theme	immunization	853:864	arg1	process					842:848	the streamlined process	826:848	the streamlined process of immunization	826:864	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	67	theme	immunization	853:864	arg1	analysis					915:922	three-dimensional structure analysis	887:922	three-dimensional structure analysis	887:922	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	67	theme	immunization	853:864	arg1	mapping					933:939	epitope mapping	925:939	epitope mapping	925:939	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	4	68	theme	monoclonal	1012:1021	arg1	antibody					1023:1030	the anti-MUC1 monoclonal antibody SN-101	998:1037	the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide	998:1079	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	3	69	theme	glycopeptidic	737:749	arg1	neoepitopes					751:761	glycopeptidic neoepitopes	737:761	glycopeptidic neoepitopes	737:761	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	5	70	theme	MUC1	1333:1336	arg1	glycopeptide					1338:1349	the MUC1 glycopeptide	1329:1349	the MUC1 glycopeptide	1329:1349	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	5	71	theme	NMR	1403:1405	arg1	conformation					1430:1441	an extended conformation	1418:1441	an extended conformation induced by site-specific glycosylation	1418:1480	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	5	71	theme	NMR	1403:1405	arg1	structure					1407:1415	its solution NMR structure	1390:1415	its solution NMR structure	1390:1415	Remarkably, the structure of the MUC1 glycopeptide in complex with SN-101 is identical to its solution NMR structure, an extended conformation induced by site-specific glycosylation.					
34122956	3	72	theme	three-dimensional	887:903	arg1	process					842:848	the streamlined process	826:848	the streamlined process of immunization	826:864	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	3	72	theme	three-dimensional	887:903	arg1	analysis					915:922	three-dimensional structure analysis	887:922	three-dimensional structure analysis	887:922	Here we present a general strategy that allows for the creation of antibodies interacting specifically with glycopeptidic neoepitopes by using homogeneous synthetic MUC1 glycopeptides designed for the streamlined process of immunization, antibody screening, three-dimensional structure analysis, epitope mapping and biochemical analysis.					
34122956	4	73	theme	anti-MUC1	1002:1010	arg1	antibody					1023:1030	the anti-MUC1 monoclonal antibody SN-101	998:1037	the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide	998:1079	The X-ray crystal structure of the anti-MUC1 monoclonal antibody SN-101 complexed with the antigenic glycopeptide provides for the first time evidence that SN-101 recognises specifically the essential epitope by forming multiple hydrogen bonds both with the proximal peptide and GalNAc linked to the threonine residue, concurrently.					
34122956	2	74	theme	extensive	359:367	arg1	efforts					369:375	extensive efforts	359:375	extensive efforts	359:375	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	6	75	theme	dynamic	1620:1626	arg1	neoepitopes					1628:1638	"dynamic neoepitopes	1619:1638	"dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins	1619:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	1	76	theme	common	245:250	arg1	feature					252:258	a common feature	243:258	a common feature of most adenocarcinomas	243:282	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	6	77	theme	mucin-like	1728:1737	arg1	sequences					1739:1747	mucin-like sequences	1728:1747	mucin-like sequences	1728:1747	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	2	78	theme	antibodies	429:438	arg1	development					403:413	the development	399:413	the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans	399:527	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	2	79	theme	anti-MUC1	533:541	arg1	antibody					543:550	no anti-MUC1 antibody	530:550	no anti-MUC1 antibody	530:550	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	1	80	dep	cancer	313:318	arg1	proliferation					320:332	proliferation	320:332	proliferation	320:332	Aberrantly truncated immature O-glycosylation in proteins occurs in essentially all types of epithelial cancer cells, which was demonstrated to be a common feature of most adenocarcinomas and strongly associated with cancer proliferation and metastasis.					
34122956	6	81	theme	class	1565:1569	arg1	development					1544:1554	the development	1540:1554	the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins	1540:1806	We demonstrate that this method accelerates dramatically the development of a new class of designated antibodies targeting a variety of "dynamic neoepitopes" elaborated by disease-specific O-glycosylation in the immunodominant mucin domains and mucin-like sequences found in intrinsically disordered regions of many proteins.					
34122956	2	82	theme	mucins	480:485	arg1	mucins					480:485	the most studied mucins	463:485	the most studied mucins having cancer-relevant immature O-glycans	463:527	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	2	82	theme	mucins	480:485	arg1	one					456:458	one	456:458	one	456:458	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
34122956	2	82	theme	mucins	480:485	arg1	MUC1					450:453	MUC1	450:453	MUC1	450:453	Although extensive efforts have been made toward the development of anticancer antibodies targeting MUC1, one of the most studied mucins having cancer-relevant immature O-glycans, no anti-MUC1 antibody recognises carbohydrates and the proximal MUC1 peptide region, concurrently.					
33064927	3	0	theme	human	721:725	arg1	ST6					752:754	ST6	752:754	ST6	752:754	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	3	0	theme	human	721:725	arg1	α-2,6-sialyltransferase					727:749	human α-2,6-sialyltransferase	721:749	human α-2,6-sialyltransferase (ST6)	721:755	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	4	1	theme	compound	915:922	arg1	half-life					891:899	the half-life	887:899	the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours	887:1056	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	1	2	from	mortality	204:212	arg1	model					226:230	a murine model	217:230	a murine model of the lethal calcification disorder generalized arterial calcification of infancy	217:313	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	3	3	theme	pH-dependent	517:528	arg1	recycling					539:547	pH-dependent cellular recycling	517:547	pH-dependent cellular recycling	517:547	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	1	4	theme	arterial	281:288	arg1	calcification					290:302	generalized arterial calcification	269:302	the lethal calcification disorder generalized arterial calcification of infancy	235:313	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	3	5	theme	1,3,4-O-Bu3	832:842	arg1	ManNAc					844:849	1,3,4-O-Bu3 ManNAc	832:849	1,3,4-O-Bu3 ManNAc	832:849	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	4	6	from	compound	915:922	arg1	37 hours					940:947	37 hours	940:947	37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours	940:1056	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	4	6	from	compound	915:922	arg1	rodents					927:933	rodents	927:933	rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours	927:1056	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	4	7	theme	parent	908:913	arg1	compound					915:922	the parent compound	904:922	the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours	904:1056	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	4	8	theme	optimized	1006:1014	arg1	recycling					1032:1040	optimized pH-dependent Fc recycling	1006:1040	optimized pH-dependent Fc recycling	1006:1040	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	2	9	theme	biomanufacturing	378:393	arg1	platform					395:402	a novel biomanufacturing platform	370:402	a novel biomanufacturing platform	370:402	We used protein engineering, glycan optimization, and a novel biomanufacturing platform to enhance potency by using a three-prong strategy.					
33064927	2	10	used	used	319:322	arg2	We					316:317	We	316:317	We	316:317	We used protein engineering, glycan optimization, and a novel biomanufacturing platform to enhance potency by using a three-prong strategy.					
33064927	5	11	theme	ENPP1-deficient	1298:1312	arg1	mice					1314:1317	ENPP1-deficient mice	1298:1317	ENPP1-deficient mice	1298:1317	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	5	12	theme	plasma	1257:1262	arg1	pyrophosphate					1281:1293	plasma phosphoanhydride pyrophosphate	1257:1293	plasma phosphoanhydride pyrophosphate	1257:1293	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	2	13	theme	protein	324:330	arg1	engineering					332:342	protein engineering	324:342	protein engineering	324:342	We used protein engineering, glycan optimization, and a novel biomanufacturing platform to enhance potency by using a three-prong strategy.					
33064927	3	14	theme	neonatal	582:589	arg1	receptor					591:598	the Fc neonatal receptor	575:598	the Fc neonatal receptor	575:598	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	3	15	used	used	613:616	arg2	we					610:611	we	610:611	we	610:611	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	0	16	theme	Glycosylation	84:96	arg1	Engineering					98:108	Glycosylation Engineering	84:108	Glycosylation Engineering	84:108	Improving the Pharmacodynamics and In Vivo Activity of ENPP1-Fc Through Protein and Glycosylation Engineering.					
33064927	1	17	theme	infancy	307:313	arg1	calcification					290:302	generalized arterial calcification	269:302	the lethal calcification disorder generalized arterial calcification of infancy	235:313	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	5	18	theme	10 days	1445:1451	arg1	week					1467:1470	every 10 days vs. 3 times a week	1439:1470	every 10 days vs. 3 times a week	1439:1470	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	3	19	theme	protein	552:558	arg1	engineering					560:570	protein engineering	552:570	protein engineering of the Fc neonatal receptor	552:598	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	4	20	from	half-life	891:899	arg1	rodents					927:933	rodents	927:933	rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours	927:1056	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	4	21	with	cells	1114:1118	arg1	supplementation					1144:1158	1,3,4-O-Bu3 ManNAc supplementation	1125:1158	1,3,4-O-Bu3 ManNAc supplementation	1125:1158	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	4	22	from	37 hours	940:947	arg1	compound					915:922	the parent compound	904:922	the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours	904:1056	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	4	22	from	37 hours	940:947	arg1	rodents					927:933	rodents	927:933	rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours	927:1056	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	3	23	theme	new	472:474	arg1	N-glycans					476:484	new N-glycans	472:484	new N-glycans	472:484	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	4	24	theme	Fc	1029:1030	arg1	recycling					1032:1040	optimized pH-dependent Fc recycling	1006:1040	optimized pH-dependent Fc recycling	1006:1040	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	1	25	with	replacement	118:128	arg1	ENPP1					186:190	ENPP1	186:190	ENPP1	186:190	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	1	25	with	replacement	118:128	arg1	phospodiesterase-1					166:183	ectonucleotide pyrophosphatase phospodiesterase-1	135:183	ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1)	135:191	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	3	26	theme	receptor	591:598	arg1	engineering					560:570	protein engineering	552:570	protein engineering of the Fc neonatal receptor	552:598	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	5	27	theme	drug	1207:1210	arg1	potency					1212:1218	drug potency	1207:1218	drug potency	1207:1218	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	2	28	theme	glycan	345:350	arg1	optimization					352:363	glycan optimization	345:363	glycan optimization	345:363	We used protein engineering, glycan optimization, and a novel biomanufacturing platform to enhance potency by using a three-prong strategy.					
33064927	4	29	with	~ 67 hours	952:961	arg1	N-glycan					977:984	an added N-glycan	968:984	an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours	968:1056	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	3	30	theme	terminal	778:785	arg1	sialylation					787:797	terminal sialylation	778:797	terminal sialylation	778:797	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	3	31	theme	Fc	579:580	arg1	receptor					591:598	the Fc neonatal receptor	575:598	the Fc neonatal receptor	575:598	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	5	32	dep	dose	1388:1391	arg1	week					1467:1470	every 10 days vs. 3 times a week	1439:1470	every 10 days vs. 3 times a week	1439:1470	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	1	33	theme	murine	219:224	arg1	model					226:230	a murine model	217:230	a murine model of the lethal calcification disorder generalized arterial calcification of infancy	217:313	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	5	34	theme	times	1459:1463	arg1	week					1467:1470	every 10 days vs. 3 times a week	1439:1470	every 10 days vs. 3 times a week	1439:1470	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	5	35	theme	phosphoanhydride	1264:1279	arg1	pyrophosphate					1281:1293	plasma phosphoanhydride pyrophosphate	1257:1293	plasma phosphoanhydride pyrophosphate	1257:1293	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	1	36	dep	disorder	260:267	arg1	calcification					290:302	generalized arterial calcification	269:302	the lethal calcification disorder generalized arterial calcification of infancy	235:313	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	1	37	theme	Enzyme	111:116	arg1	replacement					118:128	Enzyme replacement	111:128	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1)	111:191	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	6	38	theme	general	1515:1521	arg1	strategy					1523:1530	a general strategy	1513:1530	a general strategy to rationally optimize protein therapeutics	1513:1574	We believe these improvements represent a general strategy to rationally optimize protein therapeutics.					
33064927	5	39	theme	pyrophosphate	1281:1293	arg1	levels					1247:1252	efficacious levels	1235:1252	efficacious levels of plasma phosphoanhydride pyrophosphate	1235:1293	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	3	40	theme	first	663:667	arg1	ENPP1-Fc					679:686	first producing ENPP1-Fc	663:686	first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6)	663:755	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	5	41	theme	parent	1418:1423	arg1	compound-once					1425:1437	the parent compound-once	1414:1437	the parent compound-once	1414:1437	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	0	42	theme	In	35:36	arg1	Activity					43:50	In Vivo Activity	35:50	In Vivo Activity	35:50	Improving the Pharmacodynamics and In Vivo Activity of ENPP1-Fc Through Protein and Glycosylation Engineering.					
33064927	4	43	theme	added	971:975	arg1	N-glycan					977:984	an added N-glycan	968:984	an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours	968:1056	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	2	44	theme	three-prong	434:444	arg1	strategy					446:453	a three-prong strategy	432:453	a three-prong strategy	432:453	We used protein engineering, glycan optimization, and a novel biomanufacturing platform to enhance potency by using a three-prong strategy.					
33064927	3	45	from	ENPP1-Fc	679:686	arg1	cells					691:695	cells	691:695	cells stably transfected with human α-2,6-sialyltransferase (ST6)	691:755	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	2	46	theme	novel	372:376	arg1	platform					395:402	a novel biomanufacturing platform	370:402	a novel biomanufacturing platform	370:402	We used protein engineering, glycan optimization, and a novel biomanufacturing platform to enhance potency by using a three-prong strategy.					
33064927	5	47	theme	efficacious	1235:1245	arg1	levels					1247:1252	efficacious levels	1235:1252	efficacious levels of plasma phosphoanhydride pyrophosphate	1235:1293	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	5	48	theme	lower	1377:1381	arg1	dose					1388:1391	a 10-fold lower mass dose	1367:1391	a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week	1367:1470	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	0	49	dep	In	35:36	arg1	Vivo					38:41	Vivo	38:41	Vivo	38:41	Improving the Pharmacodynamics and In Vivo Activity of ENPP1-Fc Through Protein and Glycosylation Engineering.					
33064927	3	50	theme	cellular	530:537	arg1	recycling					539:547	pH-dependent cellular recycling	517:547	pH-dependent cellular recycling	517:547	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	4	51	theme	ManNAc	1137:1142	arg1	supplementation					1144:1158	1,3,4-O-Bu3 ManNAc supplementation	1125:1158	1,3,4-O-Bu3 ManNAc supplementation	1125:1158	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	1	52	theme	ectonucleotide	135:148	arg1	ENPP1					186:190	ENPP1	186:190	ENPP1	186:190	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	1	52	theme	ectonucleotide	135:148	arg1	phospodiesterase-1					166:183	ectonucleotide pyrophosphatase phospodiesterase-1	135:183	ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1)	135:191	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	4	53	theme	1,3,4-O-Bu3	1125:1135	arg1	supplementation					1144:1158	1,3,4-O-Bu3 ManNAc supplementation	1125:1158	1,3,4-O-Bu3 ManNAc supplementation	1125:1158	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	1	54	theme	lethal	239:244	arg1	disorder					260:267	the lethal calcification disorder	235:267	the lethal calcification disorder generalized arterial calcification of infancy	235:313	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	3	55	theme	producing	669:677	arg1	ENPP1-Fc					679:686	first producing ENPP1-Fc	663:686	first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6)	663:755	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	0	56	dep	Pharmacodynamics	14:29	arg1	the					10:12	the	10:12	the	10:12	Improving the Pharmacodynamics and In Vivo Activity of ENPP1-Fc Through Protein and Glycosylation Engineering.					
33064927	1	57	theme	pyrophosphatase	150:164	arg1	ENPP1					186:190	ENPP1	186:190	ENPP1	186:190	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	1	57	theme	pyrophosphatase	150:164	arg1	phospodiesterase-1					166:183	ectonucleotide pyrophosphatase phospodiesterase-1	135:183	ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1)	135:191	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	1	58	theme	calcification	246:258	arg1	disorder					260:267	the lethal calcification disorder	235:267	the lethal calcification disorder generalized arterial calcification of infancy	235:313	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	4	59	theme	pH-dependent	1016:1027	arg1	recycling					1032:1040	optimized pH-dependent Fc recycling	1006:1040	optimized pH-dependent Fc recycling	1006:1040	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	6	60	theme	protein	1555:1561	arg1	therapeutics					1563:1574	protein therapeutics	1555:1574	protein therapeutics	1555:1574	We believe these improvements represent a general strategy to rationally optimize protein therapeutics.					
33064927	4	61	with	~ 96 hours	990:999	arg1	recycling					1032:1040	optimized pH-dependent Fc recycling	1006:1040	optimized pH-dependent Fc recycling	1006:1040	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	5	62	theme	mass	1383:1386	arg1	dose					1388:1391	a 10-fold lower mass dose	1367:1391	a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week	1367:1470	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	1	63	theme	disorder	260:267	arg1	model					226:230	a murine model	217:230	a murine model of the lethal calcification disorder generalized arterial calcification of infancy	217:313	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
33064927	0	64	theme	ENPP1-Fc	55:62	arg1	Pharmacodynamics					14:29	Pharmacodynamics	14:29	Pharmacodynamics	14:29	Improving the Pharmacodynamics and In Vivo Activity of ENPP1-Fc Through Protein and Glycosylation Engineering.					
33064927	0	64	theme	ENPP1-Fc	55:62	arg1	Activity					43:50	In Vivo Activity	35:50	In Vivo Activity	35:50	Improving the Pharmacodynamics and In Vivo Activity of ENPP1-Fc Through Protein and Glycosylation Engineering.					
33064927	4	65	from	rodents	927:933	arg1	half-life					891:899	the half-life	887:899	the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours	887:1056	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	3	66	theme	two-step	620:627	arg1	process					629:635	a two-step process	618:635	a two-step process	618:635	First, we added new N-glycans to ENPP1; second, we optimized pH-dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two-step process to improve sialylation by first producing ENPP1-Fc in cells stably transfected with human α-2,6-sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4-O-Bu3 ManNAc.					
33064927	5	67	theme	a	1465:1465	arg1	week					1467:1470	every 10 days vs. 3 times a week	1439:1470	every 10 days vs. 3 times a week	1439:1470	The alterations were demonstrated to increase drug potency by maintaining efficacious levels of plasma phosphoanhydride pyrophosphate in ENPP1-deficient mice when the optimized biologic was administered at a 10-fold lower mass dose less frequently than the parent compound-once every 10 days vs. 3 times a week.					
33064927	4	68	theme	ST6-overexpressing	1095:1112	arg1	cells					1114:1118	ST6-overexpressing cells	1095:1118	ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation	1095:1158	These steps sequentially increased the half-life of the parent compound in rodents from 37 hours to ~ 67 hours with an added N-glycan, to ~ 96 hours with optimized pH-dependent Fc recycling, to ~ 204 hours when the therapeutic was produced in ST6-overexpressing cells with 1,3,4-O-Bu3 ManNAc supplementation.					
33064927	1	69	theme	generalized	269:279	arg1	calcification					290:302	generalized arterial calcification	269:302	the lethal calcification disorder generalized arterial calcification of infancy	235:313	Enzyme replacement with ectonucleotide pyrophosphatase phospodiesterase-1 (ENPP1) eliminates mortality in a murine model of the lethal calcification disorder generalized arterial calcification of infancy.					
32544586	7	0	link	-linked	1189:1195	arg1	chains					1261:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	2	1	theme	surface	386:392	arg1	method					394:399	response surface method	377:399	response surface method (RSM)	377:405	Extraction conditions were optimized by using response surface method (RSM) with a central composite design (CCD).					
32544586	2	1	theme	surface	386:392	arg1	RSM					402:404	RSM	402:404	RSM	402:404	Extraction conditions were optimized by using response surface method (RSM) with a central composite design (CCD).					
32544586	8	2	theme	bleaching	1481:1489	arg1	test					1491:1494	β-carotene bleaching test	1470:1494	β-carotene bleaching test	1470:1494	The antioxidant activities of the extract were evaluated from ABTS radical scavenging activity, total antioxidant capacity, metal-chelating power and β-carotene bleaching test.					
32544586	0	3	from	Optimization	0:11	arg1	species					55:61	a wild species	48:61	a wild species of Ornithogalum	48:77	Optimization of polysaccharides extraction from a wild species of Ornithogalum combining ultrasound and maceration and their anti-oxidant properties.					
32544586	3	4	theme	optimal	511:517	arg1	yield					535:539	the optimal polysaccharides yield	507:539	the optimal polysaccharides yield	507:539	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	3	4	theme	optimal	511:517	arg1	%					549:549	74.7%	545:549	74.7%	545:549	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	8	5	theme	radical	1387:1393	arg1	activity					1406:1413	ABTS radical scavenging activity	1382:1413	ABTS radical scavenging activity	1382:1413	The antioxidant activities of the extract were evaluated from ABTS radical scavenging activity, total antioxidant capacity, metal-chelating power and β-carotene bleaching test.					
32544586	8	6	theme	antioxidant	1324:1334	arg1	activities					1336:1345	The antioxidant activities	1320:1345	The antioxidant activities of the extract	1320:1360	The antioxidant activities of the extract were evaluated from ABTS radical scavenging activity, total antioxidant capacity, metal-chelating power and β-carotene bleaching test.					
32544586	0	7	from	species	55:61	arg1	Optimization					0:11	Optimization	0:11	Optimization of polysaccharides extraction from a wild species of Ornithogalum	0:77	Optimization of polysaccharides extraction from a wild species of Ornithogalum combining ultrasound and maceration and their anti-oxidant properties.					
32544586	0	7	from	species	55:61	arg1	extraction					32:41	polysaccharides extraction	16:41	polysaccharides extraction from a wild species of Ornithogalum	16:77	Optimization of polysaccharides extraction from a wild species of Ornithogalum combining ultrasound and maceration and their anti-oxidant properties.					
32544586	3	8	theme	polysaccharides	519:533	arg1	yield					535:539	the optimal polysaccharides yield	507:539	the optimal polysaccharides yield	507:539	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	3	8	theme	polysaccharides	519:533	arg1	%					549:549	74.7%	545:549	74.7%	545:549	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	7	9	with	fructo-polysaccharides	1140:1161	arg1	backbone					1170:1177	a backbone	1168:1177	a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1168:1266	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	5	10	theme	treatment	910:918	arg1	time					891:894	the time	887:894	the time of ultrasound treatment	887:918	The combination method also allows reducing the time of ultrasound treatment and thus its adverse effects on polysaccharides.					
32544586	5	10	theme	treatment	910:918	arg1	effects					941:947	its adverse effects	929:947	its adverse effects on polysaccharides	929:966	The combination method also allows reducing the time of ultrasound treatment and thus its adverse effects on polysaccharides.					
32544586	5	11	from	time	891:894	arg1	polysaccharides					952:966	polysaccharides	952:966	polysaccharides	952:966	The combination method also allows reducing the time of ultrasound treatment and thus its adverse effects on polysaccharides.					
32544586	1	12	theme	wild	188:191	arg1	species					193:199	a wild species	186:199	a wild species of Ornithogalum	186:215	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	9	13	theme	outstanding	1533:1543	arg1	activities					1557:1566	outstanding antioxidant activities	1533:1566	outstanding antioxidant activities	1533:1566	Data show that the extract presents outstanding antioxidant activities.					
32544586	3	14	theme	optimal	450:456	arg1	yield					469:473	The optimal extraction yield	446:473	The optimal extraction yield	446:473	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	3	14	theme	optimal	450:456	arg1	%					483:483	81.7%	479:483	81.7%	479:483	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	7	15	theme	-linked	1189:1195	arg1	chains					1261:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	5	16	theme	adverse	933:939	arg1	effects					941:947	its adverse effects	929:947	its adverse effects on polysaccharides	929:966	The combination method also allows reducing the time of ultrasound treatment and thus its adverse effects on polysaccharides.					
32544586	3	17	theme	combined	648:655	arg1	extraction					657:666	combined extraction	648:666	combined extraction	648:666	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	4	18	theme	extraction	761:770	arg1	yields					792:797	the extraction and polysaccharides yields	757:797	the extraction and polysaccharides yields	757:797	These results indicate that the combination method significantly improves the extraction and polysaccharides yields compared to traditional extraction methods.					
32544586	9	19	theme	antioxidant	1545:1555	arg1	activities					1557:1566	outstanding antioxidant activities	1533:1566	outstanding antioxidant activities	1533:1566	Data show that the extract presents outstanding antioxidant activities.					
32544586	8	20	theme	metal-chelating	1444:1458	arg1	power					1460:1464	metal-chelating power	1444:1464	metal-chelating power	1444:1464	The antioxidant activities of the extract were evaluated from ABTS radical scavenging activity, total antioxidant capacity, metal-chelating power and β-carotene bleaching test.					
32544586	1	21	theme	Ornithogalum	204:215	arg1	species					193:199	a wild species	186:199	a wild species of Ornithogalum	186:215	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	0	22	theme	extraction	32:41	arg1	Optimization					0:11	Optimization	0:11	Optimization of polysaccharides extraction from a wild species of Ornithogalum	0:77	Optimization of polysaccharides extraction from a wild species of Ornithogalum combining ultrasound and maceration and their anti-oxidant properties.					
32544586	1	23	theme	maceration	304:313	arg1	methods					232:238	three methods	226:238	three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound	226:328	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	1	23	theme	maceration	304:313	arg1	maceration					241:250	maceration	241:250	maceration	241:250	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	1	23	theme	maceration	304:313	arg1	extraction					273:282	ultrasound-assisted extraction	253:282	ultrasound-assisted extraction	253:282	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	1	23	theme	maceration	304:313	arg1	combination					289:299	combination	289:299	combination of maceration and ultrasound	289:328	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	8	24	theme	total	1416:1420	arg1	capacity					1434:1441	total antioxidant capacity	1416:1441	total antioxidant capacity	1416:1441	The antioxidant activities of the extract were evaluated from ABTS radical scavenging activity, total antioxidant capacity, metal-chelating power and β-carotene bleaching test.					
32544586	2	25	theme	Extraction	331:340	arg1	conditions					342:351	Extraction conditions	331:351	Extraction conditions	331:351	Extraction conditions were optimized by using response surface method (RSM) with a central composite design (CCD).					
32544586	8	26	theme	β-carotene	1470:1479	arg1	test					1491:1494	β-carotene bleaching test	1470:1494	β-carotene bleaching test	1470:1494	The antioxidant activities of the extract were evaluated from ABTS radical scavenging activity, total antioxidant capacity, metal-chelating power and β-carotene bleaching test.					
32544586	7	27	theme	chains	1261:1266	arg1	backbone					1170:1177	a backbone	1168:1177	a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1168:1266	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	0	28	theme	polysaccharides	16:30	arg1	extraction					32:41	polysaccharides extraction	16:41	polysaccharides extraction from a wild species of Ornithogalum	16:77	Optimization of polysaccharides extraction from a wild species of Ornithogalum combining ultrasound and maceration and their anti-oxidant properties.					
32544586	0	29	theme	anti-oxidant	125:136	arg1	properties					138:147	their anti-oxidant properties	119:147	their anti-oxidant properties	119:147	Optimization of polysaccharides extraction from a wild species of Ornithogalum combining ultrasound and maceration and their anti-oxidant properties.					
32544586	5	30	theme	ultrasound	899:908	arg1	treatment					910:918	ultrasound treatment	899:918	ultrasound treatment	899:918	The combination method also allows reducing the time of ultrasound treatment and thus its adverse effects on polysaccharides.					
32544586	7	31	theme	branched	1252:1259	arg1	chains					1261:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	1	32	theme	ultrasound	319:328	arg1	methods					232:238	three methods	226:238	three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound	226:328	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	1	32	theme	ultrasound	319:328	arg1	maceration					241:250	maceration	241:250	maceration	241:250	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	1	32	theme	ultrasound	319:328	arg1	extraction					273:282	ultrasound-assisted extraction	253:282	ultrasound-assisted extraction	253:282	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	1	32	theme	ultrasound	319:328	arg1	combination					289:299	combination	289:299	combination of maceration and ultrasound	289:328	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	7	33	dep	-linked	1189:1195	arg1	Fruf					1218:1221	Fruf	1218:1221	Fruf	1218:1221	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	7	33	dep	-linked	1189:1195	arg1	β-d-fructofuranosyl					1197:1215	β-d-fructofuranosyl	1197:1215	β-d-fructofuranosyl (Fruf)	1197:1222	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	7	34	theme	β-d-Fruf	1243:1250	arg1	chains					1261:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	8	35	theme	extract	1354:1360	arg1	activities					1336:1345	The antioxidant activities	1320:1345	The antioxidant activities of the extract	1320:1360	The antioxidant activities of the extract were evaluated from ABTS radical scavenging activity, total antioxidant capacity, metal-chelating power and β-carotene bleaching test.					
32544586	3	36	theme	extraction	458:467	arg1	yield					469:473	The optimal extraction yield	446:473	The optimal extraction yield	446:473	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	3	36	theme	extraction	458:467	arg1	%					483:483	81.7%	479:483	81.7%	479:483	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	2	37	theme	central	414:420	arg1	design					432:437	a central composite design	412:437	a central composite design (CCD)	412:443	Extraction conditions were optimized by using response surface method (RSM) with a central composite design (CCD).					
32544586	2	37	theme	central	414:420	arg1	CCD					440:442	CCD	440:442	CCD	440:442	Extraction conditions were optimized by using response surface method (RSM) with a central composite design (CCD).					
32544586	8	38	theme	antioxidant	1422:1432	arg1	capacity					1434:1441	total antioxidant capacity	1416:1441	total antioxidant capacity	1416:1441	The antioxidant activities of the extract were evaluated from ABTS radical scavenging activity, total antioxidant capacity, metal-chelating power and β-carotene bleaching test.					
32544586	7	39	dep	analysis	1093:1100	arg1	1H,13C					1063:1068	1H,13C	1063:1068	1H,13C	1063:1068	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	7	39	dep	analysis	1093:1100	arg1	HMBC					1077:1080	HMBC	1077:1080	HMBC	1077:1080	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	7	39	dep	analysis	1093:1100	arg1	COSY					1087:1090	COSY	1087:1090	COSY	1087:1090	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	7	39	dep	analysis	1093:1100	arg1	HSQC					1071:1074	HSQC	1071:1074	HSQC	1071:1074	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	2	40	theme	composite	422:430	arg1	design					432:437	a central composite design	412:437	a central composite design (CCD)	412:443	Extraction conditions were optimized by using response surface method (RSM) with a central composite design (CCD).					
32544586	2	40	theme	composite	422:430	arg1	CCD					440:442	CCD	440:442	CCD	440:442	Extraction conditions were optimized by using response surface method (RSM) with a central composite design (CCD).					
32544586	3	41	theme	optimum	579:585	arg1	conditions					587:596	the optimum conditions	575:596	the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction	575:666	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	4	42	theme	extraction	823:832	arg1	methods					834:840	traditional extraction methods	811:840	traditional extraction methods	811:840	These results indicate that the combination method significantly improves the extraction and polysaccharides yields compared to traditional extraction methods.					
32544586	2	43	theme	response	377:384	arg1	method					394:399	response surface method	377:399	response surface method (RSM)	377:405	Extraction conditions were optimized by using response surface method (RSM) with a central composite design (CCD).					
32544586	2	43	theme	response	377:384	arg1	RSM					402:404	RSM	402:404	RSM	402:404	Extraction conditions were optimized by using response surface method (RSM) with a central composite design (CCD).					
32544586	0	44	theme	wild	50:53	arg1	species					55:61	a wild species	48:61	a wild species of Ornithogalum	48:77	Optimization of polysaccharides extraction from a wild species of Ornithogalum combining ultrasound and maceration and their anti-oxidant properties.					
32544586	7	45	theme	NMR	1058:1060	arg1	analysis					1093:1100	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis	1054:1100	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis	1054:1100	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	3	46	theme	ultrasound-assisted	613:631	arg1	extraction					633:642	ultrasound-assisted extraction	613:642	ultrasound-assisted extraction	613:642	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	4	47	theme	traditional	811:821	arg1	methods					834:840	traditional extraction methods	811:840	traditional extraction methods	811:840	These results indicate that the combination method significantly improves the extraction and polysaccharides yields compared to traditional extraction methods.					
32544586	7	48	theme	glucose	1289:1295	arg1	residues					1310:1317	glucose and fructose residues	1289:1317	glucose and fructose residues	1289:1317	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	7	49	theme	-linked	1235:1241	arg1	chains					1261:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	7	50	theme	fructose	1301:1308	arg1	residues					1310:1317	glucose and fructose residues	1289:1317	glucose and fructose residues	1289:1317	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	3	51	theme	extraction	633:642	arg1	conditions					587:596	the optimum conditions	575:596	the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction	575:666	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	8	52	theme	ABTS	1382:1385	arg1	activity					1406:1413	ABTS radical scavenging activity	1382:1413	ABTS radical scavenging activity	1382:1413	The antioxidant activities of the extract were evaluated from ABTS radical scavenging activity, total antioxidant capacity, metal-chelating power and β-carotene bleaching test.					
32544586	4	53	theme	combination	715:725	arg1	method					727:732	the combination method	711:732	the combination method	711:732	These results indicate that the combination method significantly improves the extraction and polysaccharides yields compared to traditional extraction methods.					
32544586	6	54	theme	predicted	1036:1044	arg1	values					1046:1051	the theoretically predicted values	1018:1051	the theoretically predicted values	1018:1051	In addition, these results well corroborate with the theoretically predicted values.					
32544586	7	55	link	-linked	1235:1241	arg1	chains					1261:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	(2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains	1182:1266	The NMR (1H,13C, HSQC, HMBC, and COSY) analysis shows that the extract is composed of fructo-polysaccharides with a backbone of (2 → 6)-linked β-d-fructofuranosyl (Fruf) and (2 → 1)-linked β-d-Fruf branched chains, and terminated with glucose and fructose residues.					
32544586	3	56	theme	maceration	601:610	arg1	conditions					587:596	the optimum conditions	575:596	the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction	575:666	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	1	57	dep	methods	232:238	arg1	methods					232:238	three methods	226:238	three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound	226:328	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	1	57	dep	methods	232:238	arg1	maceration					241:250	maceration	241:250	maceration	241:250	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	1	57	dep	methods	232:238	arg1	extraction					273:282	ultrasound-assisted extraction	253:282	ultrasound-assisted extraction	253:282	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	1	57	dep	methods	232:238	arg1	combination					289:299	combination	289:299	combination of maceration and ultrasound	289:328	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	0	58	theme	Ornithogalum	66:77	arg1	species					55:61	a wild species	48:61	a wild species of Ornithogalum	48:77	Optimization of polysaccharides extraction from a wild species of Ornithogalum combining ultrasound and maceration and their anti-oxidant properties.					
32544586	5	59	theme	combination	847:857	arg1	method					859:864	The combination method	843:864	The combination method	843:864	The combination method also allows reducing the time of ultrasound treatment and thus its adverse effects on polysaccharides.					
32544586	8	60	theme	scavenging	1395:1404	arg1	activity					1406:1413	ABTS radical scavenging activity	1382:1413	ABTS radical scavenging activity	1382:1413	The antioxidant activities of the extract were evaluated from ABTS radical scavenging activity, total antioxidant capacity, metal-chelating power and β-carotene bleaching test.					
32544586	5	61	from	effects	941:947	arg1	polysaccharides					952:966	polysaccharides	952:966	polysaccharides	952:966	The combination method also allows reducing the time of ultrasound treatment and thus its adverse effects on polysaccharides.					
32544586	3	62	theme	extraction	657:666	arg1	conditions					587:596	the optimum conditions	575:596	the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction	575:666	The optimal extraction yield was 81.7%, 82.5% and 85.7%, and the optimal polysaccharides yield was 74.7%, 75.7%, and 82.8% under the optimum conditions of maceration, ultrasound-assisted extraction and combined extraction, respectively.					
32544586	4	63	theme	polysaccharides	776:790	arg1	yields					792:797	the extraction and polysaccharides yields	757:797	the extraction and polysaccharides yields	757:797	These results indicate that the combination method significantly improves the extraction and polysaccharides yields compared to traditional extraction methods.					
32544586	1	64	theme	ultrasound-assisted	253:271	arg1	extraction					273:282	ultrasound-assisted extraction	253:282	ultrasound-assisted extraction	253:282	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
32544586	1	64	theme	ultrasound-assisted	253:271	arg1	methods					232:238	three methods	226:238	three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound	226:328	Polysaccharides were extracted from a wild species of Ornithogalum by using three methods: maceration, ultrasound-assisted extraction, and combination of maceration and ultrasound.					
33836197	0	0	theme	acid	95:98	arg1	polysaccharides					100:114	three acid polysaccharides	89:114	three acid polysaccharides from the roots of Arctium lappa	89:146	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	5	1	dep	×	767:767	arg1	g/mol					789:793	× 104 g/mol	783:793	× 104 g/mol	783:793	ALP-3 and ALP-4 were mainly composed of fructose, arabinose and galactose, with average molecular weights of 9.709 × 104 and 6.790 × 104 g/mol, respectively.					
33836197	8	2	dep	structure	1353:1361	arg1	the					1349:1351	the	1349:1351	the	1349:1351	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	8	3	theme	functional	1475:1484	arg1	foods					1486:1490	functional foods	1475:1490	functional foods	1475:1490	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	6	4	theme	main	948:951	arg1	ALP-1					968:972	the main polysaccharide ALP-1	944:972	the main polysaccharide ALP-1	944:972	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	5	theme	spectrometry	850:861	arg1	spectroscopy					916:927	infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy	841:927	infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy	841:927	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	6	link	-linked	1077:1083	arg1	α-d-Glucopyranosyl					1085:1102	a terminal (1 → 2)-linked α-d-Glucopyranosyl	1059:1102	a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	1059:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	3	7	theme	molecular	488:496	arg1	weight					498:503	molecular weight	488:503	molecular weight	488:503	They differed in monosaccharide composition, molecular weight and linkage mode.					
33836197	1	8	theme	different	250:258	arg1	fractions					275:283	different polysaccharide fractions	250:283	different polysaccharide fractions from Arctium lappa L	250:304	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	6	9	link	-linked	1003:1009	arg1	n					1041:1041	n ≈ 15	1041:1046	n ≈ 15	1041:1046	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	9	link	-linked	1003:1009	arg1	backbone					1031:1038	(1 → 2)-linked β-D-Fructofuranosyl backbone	996:1038	(1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	996:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	5	10	theme	molecular	740:748	arg1	weights					750:756	average molecular weights	732:756	average molecular weights of 9.709 × 104 and 6.790 × 104 g/mol, respectively	732:807	ALP-3 and ALP-4 were mainly composed of fructose, arabinose and galactose, with average molecular weights of 9.709 × 104 and 6.790 × 104 g/mol, respectively.					
33836197	6	11	dep	-linked	1003:1009	arg1	→					999:999	1 → 2	997:1001	1 → 2	997:1001	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	1	12	theme	polysaccharide	260:273	arg1	fractions					275:283	different polysaccharide fractions	250:283	different polysaccharide fractions from Arctium lappa L	250:304	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	6	13	theme	infrared	841:848	arg1	spectrometry					850:861	infrared spectrometry	841:861	infrared spectrometry	841:861	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	8	14	from	information	1334:1344	arg1	structure					1353:1361	structure	1353:1361	structure	1353:1361	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	8	14	from	information	1334:1344	arg1	activity					1378:1385	biological activity	1367:1385	biological activity	1367:1385	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	1	15	theme	fractions	275:283	arg1	structure					210:218	structure	210:218	structure	210:218	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	1	15	theme	fractions	275:283	arg1	activities					236:245	antioxidant activities	224:245	antioxidant activities	224:245	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	3	16	theme	monosaccharide	460:473	arg1	composition					475:485	monosaccharide composition	460:485	monosaccharide composition	460:485	They differed in monosaccharide composition, molecular weight and linkage mode.					
33836197	7	17	theme	cell	1229:1232	arg1	model					1234:1238	H2O2-induced HepG2 cell model	1210:1238	H2O2-induced HepG2 cell model	1210:1238	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	3	18	theme	linkage	509:515	arg1	mode					517:520	linkage mode	509:520	linkage mode	509:520	They differed in monosaccharide composition, molecular weight and linkage mode.					
33836197	6	19	theme	β-D-Fructofuranosyl	1011:1029	arg1	n					1041:1041	n ≈ 15	1041:1046	n ≈ 15	1041:1046	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	19	theme	β-D-Fructofuranosyl	1011:1029	arg1	backbone					1031:1038	(1 → 2)-linked β-D-Fructofuranosyl backbone	996:1038	(1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	996:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	1	20	from	L	304:304	arg1	structure					210:218	structure	210:218	structure	210:218	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	1	20	from	L	304:304	arg1	activities					236:245	antioxidant activities	224:245	antioxidant activities	224:245	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	1	20	from	L	304:304	arg1	fractions					275:283	different polysaccharide fractions	250:283	different polysaccharide fractions from Arctium lappa L	250:304	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	6	21	theme	linear	980:985	arg1	chain					987:991	a linear chain	978:991	a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	978:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	22	theme	-linked	1003:1009	arg1	n					1041:1041	n ≈ 15	1041:1046	n ≈ 15	1041:1046	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	22	theme	-linked	1003:1009	arg1	backbone					1031:1038	(1 → 2)-linked β-D-Fructofuranosyl backbone	996:1038	(1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	996:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	2	23	theme	Arctium	426:432	arg1	L					440:440	Arctium lappa L	426:440	Arctium lappa L	426:440	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	4	24	theme	g/mol	631:635	arg1	weights					592:598	average molecular weights	574:598	average molecular weights of 2.676 × 103 and 2.503 × 104 g/mol	574:635	ALP-1 and ALP-2 mainly consisted of fructose, with average molecular weights of 2.676 × 103 and 2.503 × 104 g/mol, respectively.					
33836197	0	25	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	6	26	attach	linked	1049:1054	arg2	backbone					1031:1038	(1 → 2)-linked β-D-Fructofuranosyl backbone	996:1038	(1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	996:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	26	attach	linked	1049:1054	arg1	α-d-Glucopyranosyl					1085:1102	a terminal (1 → 2)-linked α-d-Glucopyranosyl	1059:1102	a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	1059:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	26	attach	linked	1049:1054	arg2	n					1041:1041	n ≈ 15	1041:1046	n ≈ 15	1041:1046	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	7	27	theme	-induced	1272:1279	arg1	model					1291:1295	ALP-1 in metronidazole [MET]-induced zebrafish model	1244:1295	ALP-1 in metronidazole [MET]-induced zebrafish model	1244:1295	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	8	28	theme	biological	1367:1376	arg1	activity					1378:1385	biological activity	1367:1385	biological activity	1367:1385	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	0	29	theme	antioxidant	32:42	arg1	activities					44:53	antioxidant activities	32:53	antioxidant activities	32:53	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	1	30	theme	Arctium	290:296	arg1	L					304:304	Arctium lappa L	290:304	Arctium lappa L	290:304	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	0	31	theme	Arctium	134:140	arg1	lappa					142:146	Arctium lappa	134:146	Arctium lappa	134:146	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	2	32	dep	fractions	352:360	arg1	ALP-1					363:367	ALP-1	363:367	ALP-1	363:367	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	32	dep	fractions	352:360	arg1	ALP-4					387:391	ALP-4	387:391	ALP-4	387:391	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	32	dep	fractions	352:360	arg1	fractions					352:360	four water-soluble polysaccharide fractions	318:360	four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4)	318:392	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	32	dep	fractions	352:360	arg1	ALP-3					377:381	ALP-3	377:381	ALP-3	377:381	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	32	dep	fractions	352:360	arg1	ALP-2					370:374	ALP-2	370:374	ALP-2	370:374	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	4	33	theme	×	609:609	arg1	g/mol					631:635	2.676 × 103 and 2.503 × 104 g/mol	603:635	2.676 × 103 and 2.503 × 104 g/mol	603:635	ALP-1 and ALP-2 mainly consisted of fructose, with average molecular weights of 2.676 × 103 and 2.503 × 104 g/mol, respectively.					
33836197	1	34	theme	lappa	298:302	arg1	L					304:304	Arctium lappa L	290:304	Arctium lappa L	290:304	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	6	35	theme	-linked	1077:1083	arg1	α-d-Glucopyranosyl					1085:1102	a terminal (1 → 2)-linked α-d-Glucopyranosyl	1059:1102	a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	1059:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	36	theme	backbone	1031:1038	arg1	chain					987:991	a linear chain	978:991	a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	978:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	2	37	theme	L	440:440	arg1	roots					417:421	the roots	413:421	the roots of Arctium lappa L	413:440	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	6	38	theme	≈	1043:1043	arg1	n					1041:1041	n ≈ 15	1041:1046	n ≈ 15	1041:1046	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	38	theme	≈	1043:1043	arg1	backbone					1031:1038	(1 → 2)-linked β-D-Fructofuranosyl backbone	996:1038	(1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	996:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	39	theme	resonance	906:914	arg1	spectroscopy					916:927	infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy	841:927	infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy	841:927	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	7	40	theme	high	1164:1167	arg1	ability					1181:1187	high antioxidant ability	1164:1187	high antioxidant ability	1164:1187	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	7	40	theme	high	1164:1167	arg1	ALP-4					1201:1205	ALP-4	1201:1205	especially ALP-4	1190:1205	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	5	41	theme	×	783:783	arg1	g/mol					789:793	× 104 g/mol	783:793	× 104 g/mol	783:793	ALP-3 and ALP-4 were mainly composed of fructose, arabinose and galactose, with average molecular weights of 9.709 × 104 and 6.790 × 104 g/mol, respectively.					
33836197	5	42	theme	average	732:738	arg1	weights					750:756	average molecular weights	732:756	average molecular weights of 9.709 × 104 and 6.790 × 104 g/mol, respectively	732:807	ALP-3 and ALP-4 were mainly composed of fructose, arabinose and galactose, with average molecular weights of 9.709 × 104 and 6.790 × 104 g/mol, respectively.					
33836197	6	43	theme	magnetic	897:904	arg1	resonance					906:914	nuclear magnetic resonance	889:914	nuclear magnetic resonance	889:914	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	0	44	theme	lappa	142:146	arg1	roots					125:129	the roots	121:129	the roots of Arctium lappa	121:146	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	1	45	dep	structure	210:218	arg1	the					206:208	the	206:208	the	206:208	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	5	46	theme	×	767:767	arg1	weights					750:756	average molecular weights	732:756	average molecular weights of 9.709 × 104 and 6.790 × 104 g/mol, respectively	732:807	ALP-3 and ALP-4 were mainly composed of fructose, arabinose and galactose, with average molecular weights of 9.709 × 104 and 6.790 × 104 g/mol, respectively.					
33836197	7	47	theme	H2O2-induced	1210:1221	arg1	model					1234:1238	H2O2-induced HepG2 cell model	1210:1238	H2O2-induced HepG2 cell model	1210:1238	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	0	48	theme	neutral	62:68	arg1	polysaccharide					70:83	one neutral polysaccharide	58:83	one neutral polysaccharide	58:83	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	6	49	theme	nuclear	889:895	arg1	resonance					906:914	nuclear magnetic resonance	889:914	nuclear magnetic resonance	889:914	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	8	50	theme	polysaccharides	1408:1422	arg1	structure					1353:1361	structure	1353:1361	structure	1353:1361	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	8	50	theme	polysaccharides	1408:1422	arg1	activity					1378:1385	biological activity	1367:1385	biological activity	1367:1385	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	7	51	theme	zebrafish	1281:1289	arg1	model					1291:1295	ALP-1 in metronidazole [MET]-induced zebrafish model	1244:1295	ALP-1 in metronidazole [MET]-induced zebrafish model	1244:1295	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	2	52	theme	fractions	352:360	arg1	total					309:313	A total	307:313	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4)	307:392	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	53	theme	lappa	434:438	arg1	L					440:440	Arctium lappa L	426:440	Arctium lappa L	426:440	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	1	54	from	activities	236:245	arg1	L					304:304	Arctium lappa L	290:304	Arctium lappa L	290:304	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	0	55	from	roots	125:129	arg1	activities					44:53	antioxidant activities	32:53	antioxidant activities	32:53	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	0	55	from	roots	125:129	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	0	55	from	roots	125:129	arg1	polysaccharides					100:114	three acid polysaccharides	89:114	three acid polysaccharides from the roots of Arctium lappa	89:146	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	8	56	theme	comparative	1322:1332	arg1	information					1334:1344	comparative information	1322:1344	comparative information on the structure and biological activity of different burdock polysaccharides	1322:1422	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	2	57	theme	polysaccharide	337:350	arg1	ALP-1					363:367	ALP-1	363:367	ALP-1	363:367	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	57	theme	polysaccharide	337:350	arg1	fractions					352:360	four water-soluble polysaccharide fractions	318:360	four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4)	318:392	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	57	theme	polysaccharide	337:350	arg1	ALP-4					387:391	ALP-4	387:391	ALP-4	387:391	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	57	theme	polysaccharide	337:350	arg1	ALP-3					377:381	ALP-3	377:381	ALP-3	377:381	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	57	theme	polysaccharide	337:350	arg1	ALP-2					370:374	ALP-2	370:374	ALP-2	370:374	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	6	58	theme	analysis	876:883	arg1	spectroscopy					916:927	infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy	841:927	infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy	841:927	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	1	59	from	structure	210:218	arg1	L					304:304	Arctium lappa L	290:304	Arctium lappa L	290:304	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	8	60	theme	burdock	1400:1406	arg1	polysaccharides					1408:1422	different burdock polysaccharides	1390:1422	different burdock polysaccharides	1390:1422	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	6	61	theme	non-reducing	1111:1122	arg1	end					1124:1126	the non-reducing end	1107:1126	the non-reducing end	1107:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	2	62	theme	water-soluble	323:335	arg1	ALP-1					363:367	ALP-1	363:367	ALP-1	363:367	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	62	theme	water-soluble	323:335	arg1	fractions					352:360	four water-soluble polysaccharide fractions	318:360	four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4)	318:392	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	62	theme	water-soluble	323:335	arg1	ALP-4					387:391	ALP-4	387:391	ALP-4	387:391	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	62	theme	water-soluble	323:335	arg1	ALP-3					377:381	ALP-3	377:381	ALP-3	377:381	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	2	62	theme	water-soluble	323:335	arg1	ALP-2					370:374	ALP-2	370:374	ALP-2	370:374	A total of four water-soluble polysaccharide fractions (ALP-1, ALP-2, ALP-3 and ALP-4) were obtained from the roots of Arctium lappa L.					
33836197	6	63	theme	terminal	1061:1068	arg1	α-d-Glucopyranosyl					1085:1102	a terminal (1 → 2)-linked α-d-Glucopyranosyl	1059:1102	a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	1059:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	4	64	theme	molecular	582:590	arg1	weights					592:598	average molecular weights	574:598	average molecular weights of 2.676 × 103 and 2.503 × 104 g/mol	574:635	ALP-1 and ALP-2 mainly consisted of fructose, with average molecular weights of 2.676 × 103 and 2.503 × 104 g/mol, respectively.					
33836197	6	65	contain	had	974:976	arg2	chain					987:991	a linear chain	978:991	a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	978:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	65	contain	had	974:976	arg1	ALP-1					968:972	the main polysaccharide ALP-1	944:972	the main polysaccharide ALP-1	944:972	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	8	66	from	potential	1446:1454	arg1	foods					1486:1490	functional foods	1475:1490	functional foods	1475:1490	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	6	67	dep	α-d-Glucopyranosyl	1085:1102	arg1	2					1075:1075	2	1075:1075	2	1075:1075	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	67	dep	α-d-Glucopyranosyl	1085:1102	arg1	1					1071:1071	1	1071:1071	1	1071:1071	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	4	68	theme	average	574:580	arg1	weights					592:598	average molecular weights	574:598	average molecular weights of 2.676 × 103 and 2.503 × 104 g/mol	574:635	ALP-1 and ALP-2 mainly consisted of fructose, with average molecular weights of 2.676 × 103 and 2.503 × 104 g/mol, respectively.					
33836197	8	69	theme	different	1390:1398	arg1	polysaccharides					1408:1422	different burdock polysaccharides	1390:1422	different burdock polysaccharides	1390:1422	These findings provided comparative information on the structure and biological activity of different burdock polysaccharides and highlighted their potential as antioxidants in functional foods.					
33836197	7	70	dep	-induced	1272:1279	arg1	ALP-1					1244:1248	ALP-1	1244:1248	ALP-1 in metronidazole [MET	1244:1270	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	7	71	theme	metronidazole	1253:1265	arg1	[MET					1267:1270	metronidazole [MET	1253:1270	metronidazole [MET	1253:1270	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	7	72	theme	HepG2	1223:1227	arg1	model					1234:1238	H2O2-induced HepG2 cell model	1210:1238	H2O2-induced HepG2 cell model	1210:1238	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	0	73	dep	L.	148:149	arg1	polysaccharides					100:114	three acid polysaccharides	89:114	three acid polysaccharides from the roots of Arctium lappa	89:146	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	0	73	dep	L.	148:149	arg1	comparison					154:163	A comparison	152:163	A comparison	152:163	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	0	73	dep	L.	148:149	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	0	73	dep	L.	148:149	arg1	activities					44:53	antioxidant activities	32:53	antioxidant activities	32:53	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	6	74	theme	polysaccharide	953:966	arg1	ALP-1					968:972	the main polysaccharide ALP-1	944:972	the main polysaccharide ALP-1	944:972	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	1	75	theme	antioxidant	224:234	arg1	activities					236:245	antioxidant activities	224:245	antioxidant activities	224:245	In this work, we comparatively analyzed the structure and antioxidant activities of different polysaccharide fractions from Arctium lappa L.					
33836197	0	76	theme	polysaccharide	70:83	arg1	activities					44:53	antioxidant activities	32:53	antioxidant activities	32:53	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	0	76	theme	polysaccharide	70:83	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	0	76	theme	polysaccharide	70:83	arg1	polysaccharides					100:114	three acid polysaccharides	89:114	three acid polysaccharides from the roots of Arctium lappa	89:146	Structural characterization and antioxidant activities of one neutral polysaccharide and three acid polysaccharides from the roots of Arctium lappa L.: A comparison.					
33836197	6	77	theme	methylation	864:874	arg1	analysis					876:883	methylation analysis	864:883	methylation analysis	864:883	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	6	78	from	end	1124:1126	arg1	α-d-Glucopyranosyl					1085:1102	a terminal (1 → 2)-linked α-d-Glucopyranosyl	1059:1102	a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end	1059:1126	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33836197	7	79	from	ALP-1	1244:1248	arg1	[MET					1267:1270	metronidazole [MET	1253:1270	metronidazole [MET	1253:1270	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	7	80	theme	antioxidant	1169:1179	arg1	ability					1181:1187	high antioxidant ability	1164:1187	high antioxidant ability	1164:1187	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	7	80	theme	antioxidant	1169:1179	arg1	ALP-4					1201:1205	ALP-4	1201:1205	especially ALP-4	1190:1205	All five polysaccharides displayed high antioxidant ability, especially ALP-4 in H2O2-induced HepG2 cell model and ALP-1 in metronidazole [MET]-induced zebrafish model.					
33836197	6	81	dep	Fourier	823:829	arg1	transform					831:839	transform	831:839	transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy	831:927	Furthermore, Fourier transform infrared spectrometry, methylation analysis and nuclear magnetic resonance spectroscopy suggested that the main polysaccharide ALP-1 had a linear chain of (1 → 2)-linked β-D-Fructofuranosyl backbone (n ≈ 15) linked to a terminal (1 → 2)-linked α-d-Glucopyranosyl at the non-reducing end.					
33278983	2	0	theme	pore	436:439	arg1	size					441:444	the pore size	432:444	the pore size of the composite membrane	432:470	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	1	1	theme	high	164:167	arg1	purity					169:174	high purity	164:174	high purity	164:174	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	4	2	theme	separation	826:835	arg1	efficiency					837:846	a separation efficiency	824:846	a separation efficiency (>95 %)	824:854	What's more, the membrane exhibited excellent oil-/water separation under gravity, recyclability, and a separation efficiency (>95 %), and it was both pH and salt resistant.					
33278983	4	2	theme	separation	826:835	arg1	%					853:853	>95 %	849:853	>95 %	849:853	What's more, the membrane exhibited excellent oil-/water separation under gravity, recyclability, and a separation efficiency (>95 %), and it was both pH and salt resistant.					
33278983	3	3	theme	oil	683:685	arg1	angle					695:699	the oil contact angle	679:699	the oil contact angle	679:699	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	1	4	with	material	150:157	arg1	purity					169:174	high purity	164:174	high purity	164:174	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	1	4	with	material	150:157	arg1	strength					198:205	robust mechanical strength	180:205	robust mechanical strength	180:205	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	1	5	theme	expensive	254:262	arg1	price					264:268	relatively expensive price	243:268	relatively expensive price	243:268	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	2	6	theme	membrane	463:470	arg1	size					441:444	the pore size	432:444	the pore size of the composite membrane	432:470	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	3	7	theme	contact	687:693	arg1	angle					695:699	the oil contact angle	679:699	the oil contact angle	679:699	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	2	8	theme	cellulose	355:363	arg1	needle-leaf					365:375	cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP)	341:402	cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP)	341:402	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	2	9	theme	bleached	377:384	arg1	pulp					392:395	bleached kraft pulp	377:395	cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP)	341:402	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	2	9	theme	bleached	377:384	arg1	NBKP					398:401	NBKP	398:401	NBKP	398:401	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	2	10	theme	composite	453:461	arg1	membrane					463:470	the composite membrane	449:470	the composite membrane	449:470	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	0	11	theme	oil/water	88:96	arg1	separation					98:107	gravity-driven oil/water separation	73:107	gravity-driven oil/water separation	73:107	Sustainable, superhydrophobic membranes based on bacterial cellulose for gravity-driven oil/water separation.					
33278983	1	12	theme	robust	180:185	arg1	strength					198:205	robust mechanical strength	180:205	robust mechanical strength	180:205	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	0	13	theme	Sustainable	0:10	arg1	membranes					30:38	Sustainable, superhydrophobic membranes	0:38	Sustainable, superhydrophobic membranes	0:38	Sustainable, superhydrophobic membranes based on bacterial cellulose for gravity-driven oil/water separation.					
33278983	1	14	theme	Bacterial	110:118	arg1	material					150:157	a substrate material	138:157	a substrate material with high purity and robust mechanical strength	138:205	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	1	14	theme	Bacterial	110:118	arg1	BC					131:132	BC	131:132	BC	131:132	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	1	14	theme	Bacterial	110:118	arg1	cellulose					120:128	Bacterial cellulose	110:128	Bacterial cellulose (BC)	110:133	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	1	15	theme	mechanical	187:196	arg1	strength					198:205	robust mechanical strength	180:205	robust mechanical strength	180:205	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	5	16	theme	separation	952:961	arg1	cycles					963:968	10 separation cycles	949:968	10 separation cycles	949:968	The membrane also remained durably hydrophobic after 10 separation cycles.					
33278983	4	17	theme	excellent	758:766	arg1	separation					779:788	excellent oil-/water separation	758:788	excellent oil-/water separation	758:788	What's more, the membrane exhibited excellent oil-/water separation under gravity, recyclability, and a separation efficiency (>95 %), and it was both pH and salt resistant.					
33278983	2	18	theme	cheaper	341:347	arg1	needle-leaf					365:375	cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP)	341:402	cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP)	341:402	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	2	19	theme	superhydrophobic/superoleophilic	479:510	arg1	membrane					512:519	a superhydrophobic/superoleophilic membrane	477:519	a superhydrophobic/superoleophilic membrane	477:519	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	3	20	theme	water	636:640	arg1	angle					650:654	a water contact angle	634:654	a water contact angle (WCA)	634:660	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	3	20	theme	water	636:640	arg1	WCA					657:659	WCA	657:659	WCA	657:659	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	3	21	theme	modified	565:572	arg1	surface					583:589	The modified membrane surface	561:589	The modified membrane surface	561:589	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	2	22	theme	plant	349:353	arg1	needle-leaf					365:375	cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP)	341:402	cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP)	341:402	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	2	23	dep	needle-leaf	365:375	arg1	pulp					392:395	bleached kraft pulp	377:395	cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP)	341:402	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	2	23	dep	needle-leaf	365:375	arg1	NBKP					398:401	NBKP	398:401	NBKP	398:401	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	3	24	theme	membrane	574:581	arg1	surface					583:589	The modified membrane surface	561:589	The modified membrane surface	561:589	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	2	25	theme	kraft	386:390	arg1	pulp					392:395	bleached kraft pulp	377:395	cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP)	341:402	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	2	25	theme	kraft	386:390	arg1	NBKP					398:401	NBKP	398:401	NBKP	398:401	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	6	26	theme	separation	979:988	arg1	methodology					990:1000	the separation methodology	975:1000	the separation methodology	975:1000	And the separation methodology is expected to be highly energy-efficient.					
33278983	6	26	theme	separation	979:988	arg1	energy-efficient					1027:1042	energy-efficient	1027:1042	energy-efficient	1027:1042	And the separation methodology is expected to be highly energy-efficient.					
33278983	0	27	theme	bacterial	49:57	arg1	cellulose					59:67	bacterial cellulose	49:67	bacterial cellulose for gravity-driven oil/water separation	49:107	Sustainable, superhydrophobic membranes based on bacterial cellulose for gravity-driven oil/water separation.					
33278983	2	28	theme	oil-/water	538:547	arg1	separation					549:558	oil-/water separation	538:558	oil-/water separation	538:558	In this study, cheaper plant cellulose needle-leaf bleached kraft pulp (NBKP) was added to BC to increase the pore size of the composite membrane, and a superhydrophobic/superoleophilic membrane was prepared for oil-/water separation.					
33278983	4	29	theme	oil-/water	768:777	arg1	separation					779:788	excellent oil-/water separation	758:788	excellent oil-/water separation	758:788	What's more, the membrane exhibited excellent oil-/water separation under gravity, recyclability, and a separation efficiency (>95 %), and it was both pH and salt resistant.					
33278983	3	30	theme	162.3°	665:670	arg1	angle					650:654	a water contact angle	634:654	a water contact angle (WCA)	634:660	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	3	30	theme	162.3°	665:670	arg1	micro-structure					614:628	a petal-like micro-structure	601:628	a petal-like micro-structure	601:628	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	3	30	theme	162.3°	665:670	arg1	WCA					657:659	WCA	657:659	WCA	657:659	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	1	31	theme	oil-/water	294:303	arg1	it					271:272	it	271:272	it	271:272	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	1	31	theme	oil-/water	294:303	arg1	membrane					316:323	an oil-/water separation membrane	291:323	an oil-/water separation membrane	291:323	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	3	32	theme	contact	642:648	arg1	angle					650:654	a water contact angle	634:654	a water contact angle (WCA)	634:660	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	3	32	theme	contact	642:648	arg1	WCA					657:659	WCA	657:659	WCA	657:659	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	3	33	theme	petal-like	603:612	arg1	micro-structure					614:628	a petal-like micro-structure	601:628	a petal-like micro-structure	601:628	The modified membrane surface displayed a petal-like micro-structure and a water contact angle (WCA) of 162.3°, while the oil contact angle was decreased to 0°.					
33278983	4	34	dep	both	868:871	arg1	pH					873:874	pH	873:874	pH	873:874	What's more, the membrane exhibited excellent oil-/water separation under gravity, recyclability, and a separation efficiency (>95 %), and it was both pH and salt resistant.					
33278983	0	35	theme	gravity-driven	73:86	arg1	separation					98:107	gravity-driven oil/water separation	73:107	gravity-driven oil/water separation	73:107	Sustainable, superhydrophobic membranes based on bacterial cellulose for gravity-driven oil/water separation.					
33278983	4	36	dep	exhibited	748:756	arg1	's					726:727	's	726:727	's	726:727	What's more, the membrane exhibited excellent oil-/water separation under gravity, recyclability, and a separation efficiency (>95 %), and it was both pH and salt resistant.					
33278983	1	37	theme	substrate	140:148	arg1	material					150:157	a substrate material	138:157	a substrate material with high purity and robust mechanical strength	138:205	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	1	37	theme	substrate	140:148	arg1	cellulose					120:128	Bacterial cellulose	110:128	Bacterial cellulose (BC)	110:133	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	1	38	theme	small	223:227	arg1	size					234:237	its small pore size	219:237	its small pore size	219:237	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	1	39	theme	separation	305:314	arg1	it					271:272	it	271:272	it	271:272	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	1	39	theme	separation	305:314	arg1	membrane					316:323	an oil-/water separation membrane	291:323	an oil-/water separation membrane	291:323	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
33278983	0	40	dep	Sustainable	0:10	arg1	superhydrophobic					13:28	superhydrophobic	13:28	superhydrophobic	13:28	Sustainable, superhydrophobic membranes based on bacterial cellulose for gravity-driven oil/water separation.					
33278983	1	41	theme	pore	229:232	arg1	size					234:237	its small pore size	219:237	its small pore size	219:237	Bacterial cellulose (BC) is a substrate material with high purity and robust mechanical strength, but due to its small pore size and relatively expensive price, it is restricted as an oil-/water separation membrane.					
34523784	7	0	gly	glycopeptide	836:847	arg2	glycopeptide					836:847	glycopeptide	836:847	glycopeptide	836:847	Enzymatic introduction of the sialic acids was readily accomplished at the level of the glycopeptide segments but even more efficiently on the refolded glycoprotein.					
34523784	8	1	theme	EPOs	954:957	arg1	recognition					925:935	Biological recognition	914:935	Biological recognition of the synthetic EPOs	914:957	Biological recognition of the synthetic EPOs was shown by formation of 1:1 complexes with recombinant EPO receptor.					
34523784	6	2	gly	glycopeptides	670:682	arg2	glycopeptides					670:682	The glycopeptides	666:682	The glycopeptides	666:682	The glycopeptides were obtained by pseudoproline-assisted Lansbury aspartylation.					
34523784	7	3	theme	sialic	778:783	arg1	acids					785:789	the sialic acids	774:789	the sialic acids	774:789	Enzymatic introduction of the sialic acids was readily accomplished at the level of the glycopeptide segments but even more efficiently on the refolded glycoprotein.					
34523784	8	4	theme	Biological	914:923	arg1	recognition					925:935	Biological recognition	914:935	Biological recognition of the synthetic EPOs	914:957	Biological recognition of the synthetic EPOs was shown by formation of 1:1 complexes with recombinant EPO receptor.					
34523784	7	5	theme	acids	785:789	arg1	introduction					758:769	Enzymatic introduction	748:769	Enzymatic introduction of the sialic acids	748:789	Enzymatic introduction of the sialic acids was readily accomplished at the level of the glycopeptide segments but even more efficiently on the refolded glycoprotein.					
34523784	4	6	theme	EPO	526:528	arg1	glycoforms					512:521	glycoforms	512:521	glycoforms of EPO with and without sialic acids	512:558	We have developed a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids.					
34523784	3	7	theme	pure	397:400	arg1	glycoforms					402:411	obtaining pure glycoforms	387:411	obtaining pure glycoforms	387:411	EPO carries three N-glycans and thus obtaining pure glycoforms provides a major challenge.					
34523784	8	8	theme	EPO	1016:1018	arg1	receptor					1020:1027	recombinant EPO receptor	1004:1027	recombinant EPO receptor	1004:1027	Biological recognition of the synthetic EPOs was shown by formation of 1:1 complexes with recombinant EPO receptor.					
34523784	4	9	theme	chemoenzymatic	485:498	arg1	approach					500:507	a robust and reproducible chemoenzymatic approach	459:507	a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids	459:558	We have developed a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids.					
34523784	4	10	gly	glycoforms	512:521	arg1	EPO					526:528	EPO	526:528	EPO	526:528	We have developed a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids.					
34523784	4	11	theme	reproducible	472:483	arg1	approach					500:507	a robust and reproducible chemoenzymatic approach	459:507	a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids	459:558	We have developed a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids.					
34523784	5	12	theme	peptide	625:631	arg1	ligation					609:616	sequential native chemical ligation	582:616	sequential native chemical ligation of two peptide and three glycopeptide segments	582:663	EPO was assembled by sequential native chemical ligation of two peptide and three glycopeptide segments.					
34523784	7	13	gly	glycoprotein	900:911	arg1	glycoprotein					900:911	the refolded glycoprotein	887:911	the refolded glycoprotein	887:911	Enzymatic introduction of the sialic acids was readily accomplished at the level of the glycopeptide segments but even more efficiently on the refolded glycoprotein.					
34523784	1	14	theme	Recombinant	83:93	arg1	erythropoietin					101:114	Recombinant human erythropoietin	83:114	Recombinant human erythropoietin (EPO)	83:120	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	1	14	theme	Recombinant	83:93	arg1	glycoprotein					146:157	the main therapeutic glycoprotein	125:157	the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients	125:215	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	1	14	theme	Recombinant	83:93	arg1	EPO					117:119	EPO	117:119	EPO	117:119	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	7	15	theme	refolded	891:898	arg1	glycoprotein					900:911	the refolded glycoprotein	887:911	the refolded glycoprotein	887:911	Enzymatic introduction of the sialic acids was readily accomplished at the level of the glycopeptide segments but even more efficiently on the refolded glycoprotein.					
34523784	3	16	theme	obtaining	387:395	arg1	glycoforms					402:411	obtaining pure glycoforms	387:411	obtaining pure glycoforms	387:411	EPO carries three N-glycans and thus obtaining pure glycoforms provides a major challenge.					
34523784	5	17	theme	glycopeptide	643:654	arg1	segments					656:663	two peptide and three glycopeptide segments	621:663	segments	656:663	EPO was assembled by sequential native chemical ligation of two peptide and three glycopeptide segments.					
34523784	5	18	theme	sequential	582:591	arg1	ligation					609:616	sequential native chemical ligation	582:616	sequential native chemical ligation of two peptide and three glycopeptide segments	582:663	EPO was assembled by sequential native chemical ligation of two peptide and three glycopeptide segments.					
34523784	1	19	theme	human	95:99	arg1	erythropoietin					101:114	Recombinant human erythropoietin	83:114	Recombinant human erythropoietin (EPO)	83:120	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	1	19	theme	human	95:99	arg1	glycoprotein					146:157	the main therapeutic glycoprotein	125:157	the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients	125:215	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	1	19	theme	human	95:99	arg1	EPO					117:119	EPO	117:119	EPO	117:119	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	1	20	theme	anemia	180:185	arg1	treatment					167:175	the treatment	163:175	the treatment of anemia in cancer and kidney patients	163:215	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	0	21	theme	Chemical	0:7	arg1	Synthesis					23:31	Chemical and Enzymatic Synthesis	0:31	Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin	0:80	Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin.					
34523784	5	22	theme	segments	656:663	arg1	ligation					609:616	sequential native chemical ligation	582:616	sequential native chemical ligation of two peptide and three glycopeptide segments	582:663	EPO was assembled by sequential native chemical ligation of two peptide and three glycopeptide segments.					
34523784	5	23	theme	native	593:598	arg1	ligation					609:616	sequential native chemical ligation	582:616	sequential native chemical ligation of two peptide and three glycopeptide segments	582:663	EPO was assembled by sequential native chemical ligation of two peptide and three glycopeptide segments.					
34523784	2	24	theme	residues	303:310	arg1	number					281:286	the number	277:286	the number of sialic acid residues regulating its circulatory half-life	277:347	The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life.					
34523784	6	25	theme	Lansbury	724:731	arg1	aspartylation					733:745	pseudoproline-assisted Lansbury aspartylation	701:745	pseudoproline-assisted Lansbury aspartylation	701:745	The glycopeptides were obtained by pseudoproline-assisted Lansbury aspartylation.					
34523784	5	26	theme	chemical	600:607	arg1	ligation					609:616	sequential native chemical ligation	582:616	sequential native chemical ligation of two peptide and three glycopeptide segments	582:663	EPO was assembled by sequential native chemical ligation of two peptide and three glycopeptide segments.					
34523784	2	27	theme	acid	298:301	arg1	residues					303:310	sialic acid residues	291:310	sialic acid residues regulating its circulatory half-life	291:347	The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life.					
34523784	6	28	theme	pseudoproline-assisted	701:722	arg1	aspartylation					733:745	pseudoproline-assisted Lansbury aspartylation	701:745	pseudoproline-assisted Lansbury aspartylation	701:745	The glycopeptides were obtained by pseudoproline-assisted Lansbury aspartylation.					
34523784	1	29	theme	cancer	190:195	arg1	patients					208:215	cancer and kidney patients	190:215	patients	208:215	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	0	30	theme	Enzymatic	13:21	arg1	Synthesis					23:31	Chemical and Enzymatic Synthesis	0:31	Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin	0:80	Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin.					
34523784	7	31	theme	glycopeptide	836:847	arg1	segments					849:856	the glycopeptide segments	832:856	the glycopeptide segments	832:856	Enzymatic introduction of the sialic acids was readily accomplished at the level of the glycopeptide segments but even more efficiently on the refolded glycoprotein.					
34523784	7	32	theme	Enzymatic	748:756	arg1	introduction					758:769	Enzymatic introduction	748:769	Enzymatic introduction of the sialic acids	748:789	Enzymatic introduction of the sialic acids was readily accomplished at the level of the glycopeptide segments but even more efficiently on the refolded glycoprotein.					
34523784	2	33	theme	sialic	291:296	arg1	residues					303:310	sialic acid residues	291:310	sialic acid residues regulating its circulatory half-life	291:347	The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life.					
34523784	0	34	theme	Sialylated	36:45	arg1	Glycoforms					47:56	Sialylated Glycoforms	36:56	Sialylated Glycoforms of Human Erythropoietin	36:80	Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin.					
34523784	7	35	theme	segments	849:856	arg1	level					823:827	the level	819:827	the level of the glycopeptide segments	819:856	Enzymatic introduction of the sialic acids was readily accomplished at the level of the glycopeptide segments but even more efficiently on the refolded glycoprotein.					
34523784	1	36	theme	kidney	201:206	arg1	patients					208:215	cancer and kidney patients	190:215	patients	208:215	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	3	37	theme	major	424:428	arg1	challenge					430:438	a major challenge	422:438	a major challenge	422:438	EPO carries three N-glycans and thus obtaining pure glycoforms provides a major challenge.					
34523784	2	38	theme	in-vivo	222:228	arg1	activity					230:237	The in-vivo activity	218:237	The in-vivo activity of EPO	218:244	The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life.					
34523784	2	38	theme	in-vivo	222:228	arg1	carbohydrate-dependent					249:270	carbohydrate-dependent	249:270	carbohydrate-dependent	249:270	The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life.					
34523784	3	39	contain	carries	354:360	arg2	N-glycans					368:376	three N-glycans	362:376	three N-glycans	362:376	EPO carries three N-glycans and thus obtaining pure glycoforms provides a major challenge.					
34523784	3	39	contain	carries	354:360	arg1	EPO					350:352	EPO	350:352	EPO	350:352	EPO carries three N-glycans and thus obtaining pure glycoforms provides a major challenge.					
34523784	5	40	gly	glycopeptide	643:654	arg2	glycopeptide					643:654	two peptide and three glycopeptide segments	621:663	glycopeptide	643:654	EPO was assembled by sequential native chemical ligation of two peptide and three glycopeptide segments.					
34523784	1	41	gly	glycoprotein	146:157	arg1	glycoprotein					146:157	the main therapeutic glycoprotein	125:157	the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients	125:215	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	1	41	gly	glycoprotein	146:157	arg1	erythropoietin					101:114	Recombinant human erythropoietin	83:114	Recombinant human erythropoietin (EPO)	83:120	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	4	42	theme	robust	461:466	arg1	approach					500:507	a robust and reproducible chemoenzymatic approach	459:507	a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids	459:558	We have developed a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids.					
34523784	0	43	theme	Glycoforms	47:56	arg1	Synthesis					23:31	Chemical and Enzymatic Synthesis	0:31	Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin	0:80	Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin.					
34523784	2	44	with	carbohydrate-dependent	249:270	arg1	number					281:286	the number	277:286	the number of sialic acid residues regulating its circulatory half-life	277:347	The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life.					
34523784	8	45	theme	complexes	989:997	arg1	formation					972:980	formation	972:980	formation of 1:1 complexes with recombinant EPO receptor	972:1027	Biological recognition of the synthetic EPOs was shown by formation of 1:1 complexes with recombinant EPO receptor.					
34523784	4	46	theme	sialic	547:552	arg1	acids					554:558	sialic acids	547:558	sialic acids	547:558	We have developed a robust and reproducible chemoenzymatic approach to glycoforms of EPO with and without sialic acids.					
34523784	8	47	theme	1:1	985:987	arg1	complexes					989:997	1:1 complexes	985:997	1:1 complexes	985:997	Biological recognition of the synthetic EPOs was shown by formation of 1:1 complexes with recombinant EPO receptor.					
34523784	2	48	theme	circulatory	327:337	arg1	half-life					339:347	its circulatory half-life	323:347	its circulatory half-life	323:347	The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life.					
34523784	8	49	theme	recombinant	1004:1014	arg1	receptor					1020:1027	recombinant EPO receptor	1004:1027	recombinant EPO receptor	1004:1027	Biological recognition of the synthetic EPOs was shown by formation of 1:1 complexes with recombinant EPO receptor.					
34523784	1	50	theme	main	129:132	arg1	glycoprotein					146:157	the main therapeutic glycoprotein	125:157	the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients	125:215	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	1	50	theme	main	129:132	arg1	erythropoietin					101:114	Recombinant human erythropoietin	83:114	Recombinant human erythropoietin (EPO)	83:120	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	8	51	theme	synthetic	944:952	arg1	EPOs					954:957	the synthetic EPOs	940:957	the synthetic EPOs	940:957	Biological recognition of the synthetic EPOs was shown by formation of 1:1 complexes with recombinant EPO receptor.					
34523784	0	52	theme	Erythropoietin	67:80	arg1	Glycoforms					47:56	Sialylated Glycoforms	36:56	Sialylated Glycoforms of Human Erythropoietin	36:80	Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin.					
34523784	2	53	theme	EPO	242:244	arg1	activity					230:237	The in-vivo activity	218:237	The in-vivo activity of EPO	218:244	The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life.					
34523784	2	53	theme	EPO	242:244	arg1	carbohydrate-dependent					249:270	carbohydrate-dependent	249:270	carbohydrate-dependent	249:270	The in-vivo activity of EPO is carbohydrate-dependent with the number of sialic acid residues regulating its circulatory half-life.					
34523784	1	54	theme	therapeutic	134:144	arg1	glycoprotein					146:157	the main therapeutic glycoprotein	125:157	the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients	125:215	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	1	54	theme	therapeutic	134:144	arg1	erythropoietin					101:114	Recombinant human erythropoietin	83:114	Recombinant human erythropoietin (EPO)	83:120	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
34523784	0	55	theme	Human	61:65	arg1	Erythropoietin					67:80	Human Erythropoietin	61:80	Human Erythropoietin	61:80	Chemical and Enzymatic Synthesis of Sialylated Glycoforms of Human Erythropoietin.					
34523784	8	56	with	formation	972:980	arg1	receptor					1020:1027	recombinant EPO receptor	1004:1027	recombinant EPO receptor	1004:1027	Biological recognition of the synthetic EPOs was shown by formation of 1:1 complexes with recombinant EPO receptor.					
34523784	1	57	from	treatment	167:175	arg1	patients					208:215	cancer and kidney patients	190:215	patients	208:215	Recombinant human erythropoietin (EPO) is the main therapeutic glycoprotein for the treatment of anemia in cancer and kidney patients.					
32423302	6	0	theme	fabric-reinforced	1584:1600	arg1	candidate					1706:1714	a good candidate	1699:1714	a good candidate for being employed as a dural substitute	1699:1755	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	6	0	theme	fabric-reinforced	1584:1600	arg1	composite					1602:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	2	1	theme	regenerated	422:432	arg1	cellulose					434:442	newly developed bilayer oxidized regenerated cellulose	389:442	newly developed bilayer oxidized regenerated cellulose	389:442	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	6	2	theme	ε-caprolactone	1561:1574	arg1	candidate					1706:1714	a good candidate	1699:1714	a good candidate for being employed as a dural substitute	1699:1755	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	6	2	theme	ε-caprolactone	1561:1574	arg1	composite					1602:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	4	3	theme	composites	1074:1083	arg1	Density					926:932	Density	926:932	Density	926:932	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	4	3	theme	composites	1074:1083	arg1	properties					943:952	tensile properties	935:952	tensile properties	935:952	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	4	3	theme	composites	1074:1083	arg1	strength					970:977	stitch tear strength	958:977	stitch tear strength	958:977	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	1	4	contain	have	191:194	arg1	dural					167:171	dural	167:171	dural	167:171	Ideally, alloplastic dural substitute should have functional properties resembling human dura mater and retain a watertight closure to prevent cerebrospinal leakage.					
32423302	1	4	contain	have	191:194	arg2	properties					207:216	functional properties	196:216	functional properties resembling human dura mater	196:244	Ideally, alloplastic dural substitute should have functional properties resembling human dura mater and retain a watertight closure to prevent cerebrospinal leakage.					
32423302	6	5	theme	knitted	1541:1547	arg1	candidate					1706:1714	a good candidate	1699:1714	a good candidate for being employed as a dural substitute	1699:1755	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	6	5	theme	knitted	1541:1547	arg1	composite					1602:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	2	6	theme	functional	323:332	arg1	properties					334:343	functional properties	323:343	functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose	323:442	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	1	7	theme	human	229:233	arg1	mater					240:244	human dura mater	229:244	human dura mater	229:244	Ideally, alloplastic dural substitute should have functional properties resembling human dura mater and retain a watertight closure to prevent cerebrospinal leakage.					
32423302	6	8	theme	regenerated	1519:1529	arg1	candidate					1706:1714	a good candidate	1699:1714	a good candidate for being employed as a dural substitute	1699:1755	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	6	8	theme	regenerated	1519:1529	arg1	composite					1602:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	4	9	dep	dural	1180:1184	arg1	substitutes					1186:1196	substitutes	1186:1196	substitutes	1186:1196	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	5	10	theme	ε-caprolactone	1319:1332	arg1	composites					1360:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	2	11	theme	dural	360:364	arg1	application					374:384	successful dural closure application	349:384	successful dural closure application of newly developed bilayer oxidized regenerated cellulose	349:442	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	2	12	theme	poly-L-lactide	663:676	arg1	patch					678:682	one synthetic poly-L-lactide patch	649:682	one synthetic poly-L-lactide patch	649:682	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	6	13	theme	oxidized	1510:1517	arg1	candidate					1706:1714	a good candidate	1699:1714	a good candidate for being employed as a dural substitute	1699:1755	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	6	13	theme	oxidized	1510:1517	arg1	composite					1602:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	5	14	theme	fabric-reinforced	1342:1358	arg1	composites					1360:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	3	15	theme	knitted	734:740	arg1	composites					795:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	1	16	theme	watertight	259:268	arg1	closure					270:276	a watertight closure	257:276	a watertight closure to prevent cerebrospinal leakage	257:309	Ideally, alloplastic dural substitute should have functional properties resembling human dura mater and retain a watertight closure to prevent cerebrospinal leakage.					
32423302	4	17	theme	cadaveric	1118:1126	arg1	mater					1133:1137	human cadaveric dura mater	1112:1137	human cadaveric dura mater than those of dense-type and porous-type dural substitutes	1112:1196	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	6	18	theme	functional	1626:1635	arg1	properties					1637:1646	better functional properties	1619:1646	better functional properties	1619:1646	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	5	19	theme	regenerated	1277:1287	arg1	composites					1360:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	3	20	theme	regenerated	712:722	arg1	composites					795:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	7	21	dep	properties	1851:1860	arg1	the					1836:1838	the	1836:1838	the	1836:1838	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	5	22	theme	knitted	1299:1305	arg1	composites					1360:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	3	23	theme	fabric-reinforced	777:793	arg1	composites					795:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	6	24	theme	good	1701:1704	arg1	composite					1602:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	6	24	theme	good	1701:1704	arg1	candidate					1706:1714	a good candidate	1699:1714	a good candidate for being employed as a dural substitute	1699:1755	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	5	25	theme	human	1396:1400	arg1	mater					1417:1421	human cadaveric dura mater	1396:1421	human cadaveric dura mater	1396:1421	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	3	26	theme	ε-caprolactone	754:767	arg1	composites					795:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	7	27	theme	dural	1889:1893	arg1	tightness					1872:1880	water tightness	1866:1880	water tightness	1866:1880	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	7	27	theme	dural	1889:1893	arg1	properties					1851:1860	functional properties	1840:1860	functional properties	1840:1860	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	5	28	theme	dura	1412:1415	arg1	mater					1417:1421	human cadaveric dura mater	1396:1421	human cadaveric dura mater	1396:1421	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	1	29	dep	dural	167:171	arg1	substitute					173:182	substitute	173:182	substitute	173:182	Ideally, alloplastic dural substitute should have functional properties resembling human dura mater and retain a watertight closure to prevent cerebrospinal leakage.					
32423302	0	30	theme	dural	126:130	arg1	substitution					132:143	dural substitution	126:143	dural substitution	126:143	Performance evaluation of bilayer oxidized regenerated cellulose/poly ε-caprolactone knitted fabric-reinforced composites for dural substitution.					
32423302	5	31	theme	Water	1199:1203	arg1	performance					1215:1225	Water tightness performance	1199:1225	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1199:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	7	32	theme	water	1866:1870	arg1	tightness					1872:1880	water tightness	1866:1880	water tightness	1866:1880	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	2	33	theme	dura	563:566	arg1	mater					568:572	human cadaveric dura mater	547:572	human cadaveric dura mater	547:572	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	2	34	theme	knitted	479:485	arg1	composites					505:514	ε-caprolactone knitted fabric-reinforced composites	464:514	ε-caprolactone knitted fabric-reinforced composites	464:514	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	0	35	theme	Performance	0:10	arg1	evaluation					12:21	Performance evaluation	0:21	Performance evaluation of bilayer oxidized	0:41	Performance evaluation of bilayer oxidized regenerated cellulose/poly ε-caprolactone knitted fabric-reinforced composites for dural substitution.					
32423302	2	36	theme	human	547:551	arg1	mater					568:572	human cadaveric dura mater	547:572	human cadaveric dura mater	547:572	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	7	37	dep	dural	1889:1893	arg1	substitutes					1895:1905	substitutes	1895:1905	substitutes	1895:1905	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	7	38	theme	functional	1840:1849	arg1	properties					1851:1860	functional properties	1840:1860	functional properties	1840:1860	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	4	39	theme	fabric/poly	1021:1031	arg1	composites					1074:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	0	40	theme	knitted	85:91	arg1	composites					111:120	ε-caprolactone knitted fabric-reinforced composites	70:120	ε-caprolactone knitted fabric-reinforced composites for dural substitution	70:143	Performance evaluation of bilayer oxidized regenerated cellulose/poly ε-caprolactone knitted fabric-reinforced composites for dural substitution.					
32423302	4	41	theme	knitted	1048:1054	arg1	composites					1074:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	2	42	theme	oxidized	413:420	arg1	cellulose					434:442	newly developed bilayer oxidized regenerated cellulose	389:442	newly developed bilayer oxidized regenerated cellulose	389:442	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	2	43	dep	dural	595:599	arg1	substitutes					601:611	substitutes	601:611	substitutes	601:611	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	5	44	theme	oxidized	1268:1275	arg1	composites					1360:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	4	45	theme	oxidized	982:989	arg1	composites					1074:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	2	46	theme	developed	395:403	arg1	cellulose					434:442	newly developed bilayer oxidized regenerated cellulose	389:442	newly developed bilayer oxidized regenerated cellulose	389:442	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	4	47	theme	cellulose	1003:1011	arg1	composites					1074:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	7	48	theme	microstructure	1792:1805	arg1	relationship					1771:1782	relationship	1771:1782	relationship	1771:1782	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	7	48	theme	microstructure	1792:1805	arg1	role					1762:1765	role	1762:1765	role	1762:1765	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	2	49	theme	cellulose	434:442	arg1	application					374:384	successful dural closure application	349:384	successful dural closure application of newly developed bilayer oxidized regenerated cellulose	349:442	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	5	50	from	performance	1215:1225	arg1	forms					1259:1263	both sutured and non-sutured forms	1230:1263	both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1230:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	6	51	theme	knitted	1576:1582	arg1	candidate					1706:1714	a good candidate	1699:1714	a good candidate for being employed as a dural substitute	1699:1755	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	6	51	theme	knitted	1576:1582	arg1	composite					1602:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	2	52	theme	successful	349:358	arg1	application					374:384	successful dural closure application	349:384	successful dural closure application of newly developed bilayer oxidized regenerated cellulose	349:442	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	6	53	theme	fabric/poly	1549:1559	arg1	candidate					1706:1714	a good candidate	1699:1714	a good candidate for being employed as a dural substitute	1699:1755	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	6	53	theme	fabric/poly	1549:1559	arg1	composite					1602:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	4	54	theme	fabric-reinforced	1056:1072	arg1	composites					1074:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	3	55	theme	dense	904:908	arg1	microstructure					910:923	the relatively dense microstructure	889:923	the relatively dense microstructure	889:923	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	6	56	theme	cellulose	1531:1539	arg1	candidate					1706:1714	a good candidate	1699:1714	a good candidate for being employed as a dural substitute	1699:1755	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	6	56	theme	cellulose	1531:1539	arg1	composite					1602:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite	1510:1610	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	1	57	theme	dura	235:238	arg1	mater					240:244	human dura mater	229:244	human dura mater	229:244	Ideally, alloplastic dural substitute should have functional properties resembling human dura mater and retain a watertight closure to prevent cerebrospinal leakage.					
32423302	7	58	from	role	1762:1765	arg1	tightness					1872:1880	water tightness	1866:1880	water tightness	1866:1880	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	7	58	from	role	1762:1765	arg1	properties					1851:1860	functional properties	1840:1860	functional properties	1840:1860	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	2	59	theme	closure	366:372	arg1	application					374:384	successful dural closure application	349:384	successful dural closure application of newly developed bilayer oxidized regenerated cellulose	349:442	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	7	60	from	relationship	1771:1782	arg1	tightness					1872:1880	water tightness	1866:1880	water tightness	1866:1880	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	7	60	from	relationship	1771:1782	arg1	properties					1851:1860	functional properties	1840:1860	functional properties	1840:1860	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	5	61	theme	knitted	1334:1340	arg1	composites					1360:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	3	62	theme	cellulose	724:732	arg1	composites					795:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	2	63	theme	synthetic	653:661	arg1	patch					678:682	one synthetic poly-L-lactide patch	649:682	one synthetic poly-L-lactide patch	649:682	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	3	64	contain	contained	815:823	arg1	composites					795:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	3	64	contain	contained	815:823	arg2	structure					835:843	a bilayer structure	825:843	a bilayer structure consisting of micropores distributed within the relatively dense microstructure	825:923	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	5	65	theme	composites	1360:1369	arg1	forms					1259:1263	both sutured and non-sutured forms	1230:1263	both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1230:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	3	66	theme	oxidized	703:710	arg1	composites					795:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	6	67	dep	dural	1661:1665	arg1	substitutes					1667:1677	substitutes	1667:1677	substitutes	1667:1677	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	4	68	theme	human	1112:1116	arg1	mater					1133:1137	human cadaveric dura mater	1112:1137	human cadaveric dura mater than those of dense-type and porous-type dural substitutes	1112:1196	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	6	69	theme	better	1619:1624	arg1	properties					1637:1646	better functional properties	1619:1646	better functional properties	1619:1646	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	5	70	theme	cellulose	1289:1297	arg1	composites					1360:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	3	71	theme	knitted	769:775	arg1	composites					795:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	4	72	theme	dura	1128:1131	arg1	mater					1133:1137	human cadaveric dura mater	1112:1137	human cadaveric dura mater than those of dense-type and porous-type dural substitutes	1112:1196	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	5	73	dep	dural	1466:1470	arg1	substitutes					1472:1482	substitutes	1472:1482	substitutes	1472:1482	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	4	74	theme	stitch	958:963	arg1	strength					970:977	stitch tear strength	958:977	stitch tear strength	958:977	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	0	75	theme	fabric-reinforced	93:109	arg1	composites					111:120	ε-caprolactone knitted fabric-reinforced composites	70:120	ε-caprolactone knitted fabric-reinforced composites for dural substitution	70:143	Performance evaluation of bilayer oxidized regenerated cellulose/poly ε-caprolactone knitted fabric-reinforced composites for dural substitution.					
32423302	5	76	theme	fabric/poly	1307:1317	arg1	composites					1360:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1268:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	3	77	theme	fabric/poly	742:752	arg1	composites					795:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	703:804	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	5	78	theme	cadaveric	1402:1410	arg1	mater					1417:1421	human cadaveric dura mater	1396:1421	human cadaveric dura mater	1396:1421	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	1	79	theme	functional	196:205	arg1	properties					207:216	functional properties	196:216	functional properties resembling human dura mater	196:244	Ideally, alloplastic dural substitute should have functional properties resembling human dura mater and retain a watertight closure to prevent cerebrospinal leakage.					
32423302	5	80	theme	tightness	1205:1213	arg1	performance					1215:1225	Water tightness performance	1199:1225	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1199:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	2	81	theme	collagen	627:634	arg1	matrices					636:643	two collagen matrices	623:643	two collagen matrices	623:643	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	4	82	theme	tear	965:968	arg1	strength					970:977	stitch tear strength	958:977	stitch tear strength	958:977	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	2	83	theme	fabric-reinforced	487:503	arg1	composites					505:514	ε-caprolactone knitted fabric-reinforced composites	464:514	ε-caprolactone knitted fabric-reinforced composites	464:514	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	2	84	theme	cadaveric	553:561	arg1	mater					568:572	human cadaveric dura mater	547:572	human cadaveric dura mater	547:572	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	2	85	theme	ε-caprolactone	464:477	arg1	composites					505:514	ε-caprolactone knitted fabric-reinforced composites	464:514	ε-caprolactone knitted fabric-reinforced composites	464:514	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	0	86	theme	bilayer	26:32	arg1	evaluation					12:21	Performance evaluation	0:21	Performance evaluation of bilayer oxidized	0:41	Performance evaluation of bilayer oxidized regenerated cellulose/poly ε-caprolactone knitted fabric-reinforced composites for dural substitution.					
32423302	4	87	theme	tensile	935:941	arg1	properties					943:952	tensile properties	935:952	tensile properties	935:952	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	5	88	theme	non-sutured	1247:1257	arg1	forms					1259:1263	both sutured and non-sutured forms	1230:1263	both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1230:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	4	89	theme	knitted	1013:1019	arg1	composites					1074:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	0	90	theme	ε-caprolactone	70:83	arg1	composites					111:120	ε-caprolactone knitted fabric-reinforced composites	70:120	ε-caprolactone knitted fabric-reinforced composites for dural substitution	70:143	Performance evaluation of bilayer oxidized regenerated cellulose/poly ε-caprolactone knitted fabric-reinforced composites for dural substitution.					
32423302	7	91	dep	role	1762:1765	arg1	The					1758:1760	The	1758:1760	The	1758:1760	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	6	92	dep	dural	1740:1744	arg1	substitute					1746:1755	substitute	1746:1755	substitute	1746:1755	This study revealed that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composite showed better functional properties than typical dural substitutes and was found to be a good candidate for being employed as a dural substitute.					
32423302	4	93	theme	ε-caprolactone	1033:1046	arg1	composites					1074:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	2	94	theme	bilayer	405:411	arg1	cellulose					434:442	newly developed bilayer oxidized regenerated cellulose	389:442	newly developed bilayer oxidized regenerated cellulose	389:442	Therefore, functional properties for successful dural closure application of newly developed bilayer oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were studied and compared with human cadaveric dura mater and three commercial dural substitutes including two collagen matrices and one synthetic poly-L-lactide patch.					
32423302	7	95	theme	materials	1823:1831	arg1	microstructure					1792:1805	microstructure	1792:1805	microstructure	1792:1805	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	7	95	theme	materials	1823:1831	arg1	type					1815:1818	the type	1811:1818	the type of materials	1811:1831	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	3	96	theme	bilayer	827:833	arg1	structure					835:843	a bilayer structure	825:843	a bilayer structure consisting of micropores distributed within the relatively dense microstructure	825:923	It was found that oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites uniquely contained a bilayer structure consisting of micropores distributed within the relatively dense microstructure.					
32423302	7	97	theme	type	1815:1818	arg1	relationship					1771:1782	relationship	1771:1782	relationship	1771:1782	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	7	97	theme	type	1815:1818	arg1	role					1762:1765	role	1762:1765	role	1762:1765	The role and relationship of both microstructure and the type of materials on the functional properties and water tightness of the dural substitutes were also elucidated.					
32423302	5	98	theme	sutured	1235:1241	arg1	forms					1259:1263	both sutured and non-sutured forms	1230:1263	both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	1230:1369	Water tightness performance in both sutured and non-sutured forms of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites was slightly inferior to human cadaveric dura mater, but still better than those of commercial dural substitutes.					
32423302	4	99	theme	regenerated	991:1001	arg1	composites					1074:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites	982:1083	Density, tensile properties and stitch tear strength of oxidized regenerated cellulose knitted fabric/poly ε-caprolactone knitted fabric-reinforced composites were found to be closed to human cadaveric dura mater than those of dense-type and porous-type dural substitutes.					
32423302	1	100	theme	cerebrospinal	289:301	arg1	leakage					303:309	cerebrospinal leakage	289:309	cerebrospinal leakage	289:309	Ideally, alloplastic dural substitute should have functional properties resembling human dura mater and retain a watertight closure to prevent cerebrospinal leakage.					
34464643	5	0	theme	functional	1274:1283	arg1	foods					1285:1289	functional foods	1274:1289	functional foods for antidiabetics	1274:1307	CSP-Zn exhibited higher antioxidant activities and inhibition effects on α-glucosidase than CSP, which suggested that it could be considered as a potential candidate for developing an ingredient of functional foods for antidiabetics.					
34464643	0	1	theme	corn	110:113	arg1	polysaccharide					120:133	corn silk polysaccharide	110:133	corn silk polysaccharide	110:133	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	2	2	theme	chemical	414:421	arg1	analysis					435:442	chemical composition analysis	414:442	chemical composition analysis	414:442	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	0	3	dep	complexation	42:53	arg1	III					37:39	III	37:39	III	37:39	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	1	4	with	complex	196:202	arg1	CSP					235:237	CSP	235:237	CSP	235:237	In this paper, Fe (III), Zn (II), and Cr (III) were used to complex with corn silk polysaccharide (CSP) by classical methods and CSP-Fe, CSP-Zn, and CSP-Cr were successfully synthesized, respectively.					
34464643	1	4	with	complex	196:202	arg1	polysaccharide					219:232	corn silk polysaccharide	209:232	corn silk polysaccharide (CSP)	209:238	In this paper, Fe (III), Zn (II), and Cr (III) were used to complex with corn silk polysaccharide (CSP) by classical methods and CSP-Fe, CSP-Zn, and CSP-Cr were successfully synthesized, respectively.					
34464643	2	5	theme	spectrometry	475:486	arg1	fourier					588:594	inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier	445:594	inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier	445:594	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	5	6	theme	antioxidant	1100:1110	arg1	activities					1112:1121	higher antioxidant activities	1093:1121	higher antioxidant activities	1093:1121	CSP-Zn exhibited higher antioxidant activities and inhibition effects on α-glucosidase than CSP, which suggested that it could be considered as a potential candidate for developing an ingredient of functional foods for antidiabetics.					
34464643	2	7	theme	coupled	455:461	arg1	spectrometry					475:486	inductive coupled plasma-mass spectrometry	445:486	inductive coupled plasma-mass spectrometry (ICP-MS)	445:495	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	7	theme	coupled	455:461	arg1	spectroscopy					574:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	7	theme	coupled	455:461	arg1	spectroscopy					527:538	ultraviolet-visible (UV-Vis) spectroscopy	498:538	ultraviolet-visible (UV-Vis) spectroscopy	498:538	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	7	theme	coupled	455:461	arg1	ICP-MS					489:494	ICP-MS	489:494	ICP-MS	489:494	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	0	8	theme	Cr	33:34	arg1	complexation					42:53	Cr (III) complexation	33:53	Cr (III) complexation	33:53	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	0	9	from	Effect	0:5	arg1	properties					78:87	physicochemical properties	62:87	physicochemical properties	62:87	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	0	9	from	Effect	0:5	arg1	bioactivities					93:105	bioactivities	93:105	bioactivities	93:105	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	0	10	theme	polysaccharide	120:133	arg1	properties					78:87	physicochemical properties	62:87	physicochemical properties	62:87	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	0	10	theme	polysaccharide	120:133	arg1	bioactivities					93:105	bioactivities	93:105	bioactivities	93:105	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	2	11	theme	X-ray	675:679	arg1	diffraction					681:691	X-ray diffraction	675:691	X-ray diffraction	675:691	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	4	12	theme	Zn	950:951	arg1	chelation					972:980	the Fe (III), Zn (II), and Cr (III) chelation	936:980	the Fe (III), Zn (II), and Cr (III) chelation	936:980	The results showed that the Fe (III), Zn (II), and Cr (III) chelation could change the morphology, conformation, thermostability, and biological activities of CSP.					
34464643	4	13	theme	CSP	1071:1073	arg1	thermostability					1025:1039	thermostability	1025:1039	thermostability	1025:1039	The results showed that the Fe (III), Zn (II), and Cr (III) chelation could change the morphology, conformation, thermostability, and biological activities of CSP.					
34464643	4	13	theme	CSP	1071:1073	arg1	conformation					1011:1022	conformation	1011:1022	conformation	1011:1022	The results showed that the Fe (III), Zn (II), and Cr (III) chelation could change the morphology, conformation, thermostability, and biological activities of CSP.					
34464643	4	13	theme	CSP	1071:1073	arg1	activities					1057:1066	biological activities	1046:1066	biological activities	1046:1066	The results showed that the Fe (III), Zn (II), and Cr (III) chelation could change the morphology, conformation, thermostability, and biological activities of CSP.					
34464643	4	13	theme	CSP	1071:1073	arg1	morphology					999:1008	morphology	999:1008	morphology	999:1008	The results showed that the Fe (III), Zn (II), and Cr (III) chelation could change the morphology, conformation, thermostability, and biological activities of CSP.					
34464643	0	14	theme	silk	115:118	arg1	polysaccharide					120:133	corn silk polysaccharide	110:133	corn silk polysaccharide	110:133	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	2	15	theme	UV-Vis	519:524	arg1	spectrometry					475:486	inductive coupled plasma-mass spectrometry	445:486	inductive coupled plasma-mass spectrometry (ICP-MS)	445:495	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	15	theme	UV-Vis	519:524	arg1	spectroscopy					527:538	ultraviolet-visible (UV-Vis) spectroscopy	498:538	ultraviolet-visible (UV-Vis) spectroscopy	498:538	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	5	16	theme	foods	1285:1289	arg1	ingredient					1260:1269	an ingredient	1257:1269	an ingredient of functional foods for antidiabetics	1257:1307	CSP-Zn exhibited higher antioxidant activities and inhibition effects on α-glucosidase than CSP, which suggested that it could be considered as a potential candidate for developing an ingredient of functional foods for antidiabetics.					
34464643	1	17	theme	corn	209:212	arg1	CSP					235:237	CSP	235:237	CSP	235:237	In this paper, Fe (III), Zn (II), and Cr (III) were used to complex with corn silk polysaccharide (CSP) by classical methods and CSP-Fe, CSP-Zn, and CSP-Cr were successfully synthesized, respectively.					
34464643	1	17	theme	corn	209:212	arg1	polysaccharide					219:232	corn silk polysaccharide	209:232	corn silk polysaccharide (CSP)	209:238	In this paper, Fe (III), Zn (II), and Cr (III) were used to complex with corn silk polysaccharide (CSP) by classical methods and CSP-Fe, CSP-Zn, and CSP-Cr were successfully synthesized, respectively.					
34464643	2	18	theme	ultraviolet-visible	498:516	arg1	spectrometry					475:486	inductive coupled plasma-mass spectrometry	445:486	inductive coupled plasma-mass spectrometry (ICP-MS)	445:495	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	18	theme	ultraviolet-visible	498:516	arg1	spectroscopy					527:538	ultraviolet-visible (UV-Vis) spectroscopy	498:538	ultraviolet-visible (UV-Vis) spectroscopy	498:538	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	4	19	theme	Fe	940:941	arg1	chelation					972:980	the Fe (III), Zn (II), and Cr (III) chelation	936:980	the Fe (III), Zn (II), and Cr (III) chelation	936:980	The results showed that the Fe (III), Zn (II), and Cr (III) chelation could change the morphology, conformation, thermostability, and biological activities of CSP.					
34464643	5	20	from	effects	1138:1144	arg1	α-glucosidase					1149:1161	α-glucosidase	1149:1161	α-glucosidase	1149:1161	CSP-Zn exhibited higher antioxidant activities and inhibition effects on α-glucosidase than CSP, which suggested that it could be considered as a potential candidate for developing an ingredient of functional foods for antidiabetics.					
34464643	2	21	theme	physicochemical	341:355	arg1	properties					357:366	The physicochemical properties	337:366	The physicochemical properties	337:366	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	1	22	theme	silk	214:217	arg1	CSP					235:237	CSP	235:237	CSP	235:237	In this paper, Fe (III), Zn (II), and Cr (III) were used to complex with corn silk polysaccharide (CSP) by classical methods and CSP-Fe, CSP-Zn, and CSP-Cr were successfully synthesized, respectively.					
34464643	1	22	theme	silk	214:217	arg1	polysaccharide					219:232	corn silk polysaccharide	209:232	corn silk polysaccharide (CSP)	209:238	In this paper, Fe (III), Zn (II), and Cr (III) were used to complex with corn silk polysaccharide (CSP) by classical methods and CSP-Fe, CSP-Zn, and CSP-Cr were successfully synthesized, respectively.					
34464643	5	23	theme	potential	1222:1230	arg1	candidate					1232:1240	a potential candidate	1220:1240	a potential candidate for developing an ingredient of functional foods for antidiabetics	1220:1307	CSP-Zn exhibited higher antioxidant activities and inhibition effects on α-glucosidase than CSP, which suggested that it could be considered as a potential candidate for developing an ingredient of functional foods for antidiabetics.					
34464643	5	23	theme	potential	1222:1230	arg1	it					1194:1195	it	1194:1195	it	1194:1195	CSP-Zn exhibited higher antioxidant activities and inhibition effects on α-glucosidase than CSP, which suggested that it could be considered as a potential candidate for developing an ingredient of functional foods for antidiabetics.					
34464643	2	24	theme	resonance	558:566	arg1	spectrometry					475:486	inductive coupled plasma-mass spectrometry	445:486	inductive coupled plasma-mass spectrometry (ICP-MS)	445:495	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	24	theme	resonance	558:566	arg1	spectroscopy					574:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	25	theme	FT-IR	616:620	arg1	spectroscopy					623:634	infrared (FT-IR) spectroscopy	606:634	infrared (FT-IR) spectroscopy	606:634	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	25	theme	FT-IR	616:620	arg1	DSC					775:777	DSC	775:777	DSC	775:777	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	25	theme	FT-IR	616:620	arg1	XRD					694:696	XRD	694:696	XRD	694:696	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	3	26	from	activities	811:820	arg1	α-glucosidase					848:860	α-glucosidase	848:860	α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr	848:895	The antioxidant activities and inhibitory effects on α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr were compared.					
34464643	2	27	theme	magnetic	549:556	arg1	spectrometry					475:486	inductive coupled plasma-mass spectrometry	445:486	inductive coupled plasma-mass spectrometry (ICP-MS)	445:495	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	27	theme	magnetic	549:556	arg1	spectroscopy					574:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	28	dep	transform	596:604	arg1	by					411:412	by	411:412	by	411:412	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	29	theme	scanning	753:760	arg1	calorimetry					762:772	differential scanning calorimetry	740:772	differential scanning calorimetry	740:772	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	5	30	theme	inhibition	1127:1136	arg1	effects					1138:1144	inhibition effects	1127:1144	inhibition effects on α-glucosidase	1127:1161	CSP-Zn exhibited higher antioxidant activities and inhibition effects on α-glucosidase than CSP, which suggested that it could be considered as a potential candidate for developing an ingredient of functional foods for antidiabetics.					
34464643	2	31	theme	nuclear	541:547	arg1	spectrometry					475:486	inductive coupled plasma-mass spectrometry	445:486	inductive coupled plasma-mass spectrometry (ICP-MS)	445:495	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	31	theme	nuclear	541:547	arg1	spectroscopy					574:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	3	32	theme	CSP-Cr	890:895	arg1	α-glucosidase					848:860	α-glucosidase	848:860	α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr	848:895	The antioxidant activities and inhibitory effects on α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr were compared.					
34464643	2	33	theme	differential	740:751	arg1	calorimetry					762:772	differential scanning calorimetry	740:772	differential scanning calorimetry	740:772	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	0	34	theme	complexation	42:53	arg1	Effect					0:5	Effect	0:5	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.	0:134	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	2	35	theme	dichroism	646:654	arg1	spectroscopy					661:672	circular dichroism (CD) spectroscopy	637:672	circular dichroism (CD) spectroscopy	637:672	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	3	36	theme	CSP	865:867	arg1	α-glucosidase					848:860	α-glucosidase	848:860	α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr	848:895	The antioxidant activities and inhibitory effects on α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr were compared.					
34464643	0	37	theme	Fe	10:11	arg1	Effect					0:5	Effect	0:5	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.	0:134	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	4	38	theme	Cr	963:964	arg1	chelation					972:980	the Fe (III), Zn (II), and Cr (III) chelation	936:980	the Fe (III), Zn (II), and Cr (III) chelation	936:980	The results showed that the Fe (III), Zn (II), and Cr (III) chelation could change the morphology, conformation, thermostability, and biological activities of CSP.					
34464643	2	39	theme	circular	637:644	arg1	spectroscopy					661:672	circular dichroism (CD) spectroscopy	637:672	circular dichroism (CD) spectroscopy	637:672	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	5	40	theme	higher	1093:1098	arg1	activities					1112:1121	higher antioxidant activities	1093:1121	higher antioxidant activities	1093:1121	CSP-Zn exhibited higher antioxidant activities and inhibition effects on α-glucosidase than CSP, which suggested that it could be considered as a potential candidate for developing an ingredient of functional foods for antidiabetics.					
34464643	3	41	theme	CSP-Zn	878:883	arg1	α-glucosidase					848:860	α-glucosidase	848:860	α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr	848:895	The antioxidant activities and inhibitory effects on α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr were compared.					
34464643	0	42	theme	Zn	20:21	arg1	Effect					0:5	Effect	0:5	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.	0:134	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	2	43	theme	inductive	445:453	arg1	spectrometry					475:486	inductive coupled plasma-mass spectrometry	445:486	inductive coupled plasma-mass spectrometry (ICP-MS)	445:495	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	43	theme	inductive	445:453	arg1	spectroscopy					574:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	43	theme	inductive	445:453	arg1	spectroscopy					527:538	ultraviolet-visible (UV-Vis) spectroscopy	498:538	ultraviolet-visible (UV-Vis) spectroscopy	498:538	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	43	theme	inductive	445:453	arg1	ICP-MS					489:494	ICP-MS	489:494	ICP-MS	489:494	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	4	44	theme	biological	1046:1055	arg1	activities					1057:1066	biological activities	1046:1066	biological activities	1046:1066	The results showed that the Fe (III), Zn (II), and Cr (III) chelation could change the morphology, conformation, thermostability, and biological activities of CSP.					
34464643	1	45	used	used	188:191	arg2	Zn					161:162	Zn (II)	161:167	Zn (II)	161:167	In this paper, Fe (III), Zn (II), and Cr (III) were used to complex with corn silk polysaccharide (CSP) by classical methods and CSP-Fe, CSP-Zn, and CSP-Cr were successfully synthesized, respectively.					
34464643	1	45	used	used	188:191	arg2	Cr					174:175	Cr (III)	174:181	Cr (III)	174:181	In this paper, Fe (III), Zn (II), and Cr (III) were used to complex with corn silk polysaccharide (CSP) by classical methods and CSP-Fe, CSP-Zn, and CSP-Cr were successfully synthesized, respectively.					
34464643	1	45	used	used	188:191	arg2	Fe					151:152	Fe (III)	151:158	Fe (III)	151:158	In this paper, Fe (III), Zn (II), and Cr (III) were used to complex with corn silk polysaccharide (CSP) by classical methods and CSP-Fe, CSP-Zn, and CSP-Cr were successfully synthesized, respectively.					
34464643	0	46	theme	physicochemical	62:76	arg1	properties					78:87	physicochemical properties	62:87	physicochemical properties	62:87	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	1	47	theme	classical	243:251	arg1	methods					253:259	classical methods	243:259	classical methods	243:259	In this paper, Fe (III), Zn (II), and Cr (III) were used to complex with corn silk polysaccharide (CSP) by classical methods and CSP-Fe, CSP-Zn, and CSP-Cr were successfully synthesized, respectively.					
34464643	2	48	theme	NMR	569:571	arg1	spectrometry					475:486	inductive coupled plasma-mass spectrometry	445:486	inductive coupled plasma-mass spectrometry (ICP-MS)	445:495	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	48	theme	NMR	569:571	arg1	spectroscopy					574:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	49	theme	plasma-mass	463:473	arg1	spectrometry					475:486	inductive coupled plasma-mass spectrometry	445:486	inductive coupled plasma-mass spectrometry (ICP-MS)	445:495	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	49	theme	plasma-mass	463:473	arg1	spectroscopy					574:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	nuclear magnetic resonance (NMR) spectroscopy	541:585	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	49	theme	plasma-mass	463:473	arg1	spectroscopy					527:538	ultraviolet-visible (UV-Vis) spectroscopy	498:538	ultraviolet-visible (UV-Vis) spectroscopy	498:538	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	49	theme	plasma-mass	463:473	arg1	ICP-MS					489:494	ICP-MS	489:494	ICP-MS	489:494	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	3	50	theme	CSP-Fe	870:875	arg1	α-glucosidase					848:860	α-glucosidase	848:860	α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr	848:895	The antioxidant activities and inhibitory effects on α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr were compared.					
34464643	0	51	dep	properties	78:87	arg1	the					58:60	the	58:60	the	58:60	Effect of Fe (III), Zn (II), and Cr (III) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide.					
34464643	2	52	theme	electron	709:716	arg1	SEM					730:732	SEM	730:732	SEM	730:732	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	52	theme	electron	709:716	arg1	microscopy					718:727	scanning electron microscopy	700:727	scanning electron microscopy (SEM)	700:733	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	53	theme	composition	423:433	arg1	analysis					435:442	chemical composition analysis	414:442	chemical composition analysis	414:442	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	5	54	from	activities	1112:1121	arg1	α-glucosidase					1149:1161	α-glucosidase	1149:1161	α-glucosidase	1149:1161	CSP-Zn exhibited higher antioxidant activities and inhibition effects on α-glucosidase than CSP, which suggested that it could be considered as a potential candidate for developing an ingredient of functional foods for antidiabetics.					
34464643	2	55	theme	infrared	606:613	arg1	spectroscopy					623:634	infrared (FT-IR) spectroscopy	606:634	infrared (FT-IR) spectroscopy	606:634	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	55	theme	infrared	606:613	arg1	DSC					775:777	DSC	775:777	DSC	775:777	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	55	theme	infrared	606:613	arg1	XRD					694:696	XRD	694:696	XRD	694:696	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	3	56	dep	activities	811:820	arg1	The					795:797	The	795:797	The	795:797	The antioxidant activities and inhibitory effects on α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr were compared.					
34464643	2	57	theme	scanning	700:707	arg1	SEM					730:732	SEM	730:732	SEM	730:732	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	2	57	theme	scanning	700:707	arg1	microscopy					718:727	scanning electron microscopy	700:727	scanning electron microscopy (SEM)	700:733	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	3	58	theme	inhibitory	826:835	arg1	effects					837:843	inhibitory effects	826:843	inhibitory effects	826:843	The antioxidant activities and inhibitory effects on α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr were compared.					
34464643	3	59	from	effects	837:843	arg1	α-glucosidase					848:860	α-glucosidase	848:860	α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr	848:895	The antioxidant activities and inhibitory effects on α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr were compared.					
34464643	2	60	theme	CD	657:658	arg1	spectroscopy					661:672	circular dichroism (CD) spectroscopy	637:672	circular dichroism (CD) spectroscopy	637:672	The physicochemical properties and structural features were characterized by chemical composition analysis, inductive coupled plasma-mass spectrometry (ICP-MS), ultraviolet-visible (UV-Vis) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC), respectively.					
34464643	3	61	theme	antioxidant	799:809	arg1	activities					811:820	antioxidant activities	799:820	antioxidant activities	799:820	The antioxidant activities and inhibitory effects on α-glucosidase of CSP, CSP-Fe, CSP-Zn, and CSP-Cr were compared.					
34464643	4	62	dep	morphology	999:1008	arg1	the					995:997	the	995:997	the	995:997	The results showed that the Fe (III), Zn (II), and Cr (III) chelation could change the morphology, conformation, thermostability, and biological activities of CSP.					
35126971	5	0	from	N370	1311:1314	arg1	N-glycosylation					1292:1306	N-glycosylation	1292:1306	N-glycosylation at N370	1292:1314	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	1	1	theme	S	200:200	arg1	architecture					203:214	the SARS-CoV-2 spike (S) architecture	178:214	the SARS-CoV-2 spike (S) architecture	178:214	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	1	2	theme	architecture	203:214	arg1	glycan					137:142	The dense glycan	127:142	The dense glycan shield	127:149	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	1	2	theme	architecture	203:214	arg1	key					217:219	key	217:219	key	217:219	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	1	2	theme	architecture	203:214	arg1	feature					167:173	an essential feature	154:173	an essential feature of the SARS-CoV-2 spike (S) architecture	154:214	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	2	3	dep	stability	453:461	arg1	point					480:484	a point	478:484	the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms	438:570	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	1	4	theme	dense	131:135	arg1	glycan					137:142	The dense glycan	127:142	The dense glycan shield	127:149	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	1	4	theme	dense	131:135	arg1	key					217:219	key	217:219	key	217:219	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	1	4	theme	dense	131:135	arg1	feature					167:173	an essential feature	154:173	an essential feature of the SARS-CoV-2 spike (S) architecture	154:214	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	4	5	theme	molecular	846:854	arg1	simulations					865:875	multi-microsecond molecular dynamics simulations	828:875	multi-microsecond molecular dynamics simulations	828:875	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	6	theme	open	1007:1010	arg1	conformation					1012:1023	the receptor binding domain (RBD) open conformation	973:1023	the receptor binding domain (RBD) open conformation	973:1023	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	6	7	theme	SARS-CoV-2	1456:1465	arg1	S					1467:1467	the SARS-CoV-2 S	1452:1467	the SARS-CoV-2 S	1452:1467	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	5	8	from	N370	1134:1137	arg1	loss					1109:1112	the loss	1105:1112	the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology,	1105:1214	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	3	9	with	intertwined	728:738	arg1	evolution					749:757	the evolution	745:757	the evolution of the protein sequence	745:781	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	0	10	theme	S	124:124	arg1	dynamics					97:104	dynamics	97:104	dynamics	97:104	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	0	10	theme	S	124:124	arg1	structure					83:91	structure	83:91	structure	83:91	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	6	11	theme	N370	1437:1440	arg1	glycan					1442:1447	the N370 glycan	1433:1447	the N370 glycan	1433:1447	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	2	12	theme	host	415:418	arg1	cell					420:423	the host cell	411:423	the host cell	411:423	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	4	13	theme	exposure	1039:1046	arg1	stability					960:968	the stability	956:968	the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility	956:1064	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	5	14	theme	SARS-CoV-2	1177:1186	arg1	topology					1206:1213	the SARS-CoV-2 S glycan shield's topology	1173:1213	the SARS-CoV-2 S glycan shield's topology	1173:1213	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	6	15	from	absence	1422:1428	arg1	S					1467:1467	the SARS-CoV-2 S	1452:1467	the SARS-CoV-2 S	1452:1467	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	4	16	theme	glycosylation	903:915	arg1	type					895:898	the type	891:898	the type of glycosylation at N234, N165 and N343	891:938	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	17	theme	accessibility	1052:1064	arg1	stability					960:968	the stability	956:968	the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility	956:1064	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	0	18	dep	structure	83:91	arg1	the					79:81	the	79:81	the	79:81	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	3	19	theme	protein	766:772	arg1	sequence					774:781	the protein sequence	762:781	the protein sequence	762:781	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	1	20	dep	key	217:219	arg1	glycan					137:142	The dense glycan	127:142	The dense glycan shield	127:149	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	1	20	dep	key	217:219	arg1	key					217:219	key	217:219	key	217:219	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	1	20	dep	key	217:219	arg1	feature					167:173	an essential feature	154:173	an essential feature of the SARS-CoV-2 spike (S) architecture	154:214	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	5	21	theme	acquired	1148:1155	arg1	modification					1157:1168	a newly acquired modification	1140:1168	a newly acquired modification in the SARS-CoV-2 S glycan shield's topology	1140:1213	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	5	21	theme	acquired	1148:1155	arg1	N370					1134:1137	N370	1134:1137	N370	1134:1137	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	0	22	theme	glycan	62:67	arg1	topology					46:53	topology	46:53	topology	46:53	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	0	22	theme	glycan	62:67	arg1	nature					35:40	nature	35:40	nature	35:40	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	1	23	theme	conformation	278:289	arg1	activation					250:259	the activation	246:259	the activation of the prefusion conformation	246:289	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	6	24	theme	binding	1490:1496	arg1	cleft					1498:1502	the RBD glycan binding cleft	1475:1502	the RBD glycan binding cleft	1475:1502	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	5	25	theme	RBD	1338:1340	arg1	conformation					1342:1353	the closed RBD conformation	1327:1353	the closed RBD conformation	1327:1353	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	2	26	theme	relative	528:535	arg1	competence					537:546	the relative competence	524:546	the relative competence of different glycoforms	524:570	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	6	27	theme	RBD	1479:1481	arg1	cleft					1498:1502	the RBD glycan binding cleft	1475:1502	the RBD glycan binding cleft	1475:1502	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	0	28	dep	glycan	62:67	arg1	shield					69:74	shield	69:74	shield	69:74	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	3	29	theme	optimal	793:799	arg1	activity					801:808	optimal activity	793:808	optimal activity	793:808	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	0	30	from	role	23:26	arg1	dynamics					97:104	dynamics	97:104	dynamics	97:104	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	0	30	from	role	23:26	arg1	structure					83:91	structure	83:91	structure	83:91	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	2	31	theme	S	368:368	arg1	glycans					370:376	the SARS-CoV-2 S glycans	353:376	the SARS-CoV-2 S glycans	353:376	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	2	32	theme	glycoforms	561:570	arg1	competence					537:546	the relative competence	524:546	the relative competence of different glycoforms	524:570	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	5	33	theme	glycan	1190:1195	arg1	topology					1206:1213	the SARS-CoV-2 S glycan shield's topology	1173:1213	the SARS-CoV-2 S glycan shield's topology	1173:1213	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	0	34	theme	spike	16:20	arg1	role					23:26	the spike: role	12:26	the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S	12:124	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	4	35	from	N343	935:938	arg1	type					895:898	the type	891:898	the type of glycosylation at N234, N165 and N343	891:938	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	35	from	N343	935:938	arg1	glycosylation					903:915	glycosylation	903:915	glycosylation at N234, N165 and N343	903:938	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	1	36	dep	glycan	137:142	arg1	shield					144:149	shield	144:149	shield	144:149	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	4	37	from	N165	926:929	arg1	type					895:898	the type	891:898	the type of glycosylation at N234, N165 and N343	891:938	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	37	from	N165	926:929	arg1	glycosylation					903:915	glycosylation	903:915	glycosylation at N234, N165 and N343	903:938	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	38	from	N234	920:923	arg1	type					895:898	the type	891:898	the type of glycosylation at N234, N165 and N343	891:938	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	38	from	N234	920:923	arg1	glycosylation					903:915	glycosylation	903:915	glycosylation at N234, N165 and N343	903:938	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	39	theme	binding	986:992	arg1	RBD					1002:1004	RBD	1002:1004	RBD	1002:1004	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	39	theme	binding	986:992	arg1	domain					994:999	the receptor binding domain	973:999	the receptor binding domain (RBD) open conformation	973:1023	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	2	40	dep	occupancy	325:333	arg1	the					321:323	the	321:323	the	321:323	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	4	41	from	type	895:898	arg1	N343					935:938	N343	935:938	N343	935:938	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	41	from	type	895:898	arg1	N165					926:929	N165	926:929	N165	926:929	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	41	from	type	895:898	arg1	N234					920:923	N234	920:923	N234	920:923	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	3	42	theme	functional	587:596	arg1	role					598:601	the functional role	583:601	the functional role of the glycan shield in the SARS-CoV-2 pathogenesis	583:653	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	4	43	from	simulations	865:875	arg1	results					815:821	Our results	811:821	Our results from multi-microsecond molecular dynamics simulations	811:875	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	5	44	theme	closed	1331:1336	arg1	conformation					1342:1353	the closed RBD conformation	1327:1353	the closed RBD conformation	1327:1353	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	3	45	theme	glycan	610:615	arg1	role					598:601	the functional role	583:601	the functional role of the glycan shield in the SARS-CoV-2 pathogenesis	583:653	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	2	46	theme	important	498:506	arg1	questions					508:516	important questions	498:516	important questions about the relative competence of different glycoforms	498:570	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	2	47	theme	structural	442:451	arg1	stability					453:461	the structural stability	438:461	the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms	438:570	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	4	48	theme	conformation	1012:1023	arg1	stability					960:968	the stability	956:968	the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility	956:1064	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	3	49	theme	SARS-CoV-2	631:640	arg1	pathogenesis					642:653	the SARS-CoV-2 pathogenesis	627:653	the SARS-CoV-2 pathogenesis	627:653	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	1	50	theme	essential	157:165	arg1	glycan					137:142	The dense glycan	127:142	The dense glycan shield	127:149	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	1	50	theme	essential	157:165	arg1	key					217:219	key	217:219	key	217:219	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	1	50	theme	essential	157:165	arg1	feature					167:173	an essential feature	154:173	an essential feature of the SARS-CoV-2 spike (S) architecture	154:214	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	5	51	from	cleft	1377:1381	arg1	surface					1394:1400	the RBD surface	1386:1400	the RBD surface	1386:1400	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	4	52	theme	multi-microsecond	828:844	arg1	simulations					865:875	multi-microsecond molecular dynamics simulations	828:875	multi-microsecond molecular dynamics simulations	828:875	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	6	53	theme	cell-surface	1537:1548	arg1	glycans					1550:1556	cell-surface glycans	1537:1556	cell-surface glycans	1537:1556	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	3	54	from	role	598:601	arg1	pathogenesis					642:653	the SARS-CoV-2 pathogenesis	627:653	the SARS-CoV-2 pathogenesis	627:653	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	0	55	theme	SARS-CoV-2	113:122	arg1	S					124:124	the SARS-CoV-2 S	109:124	the SARS-CoV-2 S	109:124	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	6	56	theme	cell	1590:1593	arg1	localization					1603:1614	host cell surface localization	1585:1614	host cell surface localization	1585:1614	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	2	57	theme	trimer	470:475	arg1	stability					453:461	the structural stability	438:461	the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms	438:570	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	3	58	gly	glycosylation	699:711	arg2	glycosylation					699:711	glycosylation	699:711	glycosylation	699:711	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	3	58	gly	glycosylation	699:711	arg2	sites					690:694	the sites	686:694	the sites of glycosylation	686:711	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	6	59	theme	glycan	1442:1447	arg1	absence					1422:1428	the absence	1418:1428	the absence of the N370 glycan in the SARS-CoV-2 S	1418:1467	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	3	60	theme	glycosylation	699:711	arg1	glycosylation					699:711	glycosylation	699:711	glycosylation	699:711	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	3	60	theme	glycosylation	699:711	arg1	sites					690:694	the sites	686:694	the sites of glycosylation	686:711	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	0	61	theme	topology	46:53	arg1	role					23:26	the spike: role	12:26	the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S	12:124	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	3	62	theme	sites	690:694	arg1	intertwined					728:738	intertwined	728:738	intertwined	728:738	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	3	62	theme	sites	690:694	arg1	evolution					673:681	the evolution	669:681	the evolution of the sites of glycosylation	669:711	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	6	63	theme	surface	1595:1601	arg1	localization					1603:1614	host cell surface localization	1585:1614	host cell surface localization	1585:1614	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	5	64	theme	specific	1368:1375	arg1	cleft					1377:1381	a specific cleft	1366:1381	a specific cleft on the RBD surface	1366:1400	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	2	65	theme	different	551:559	arg1	glycoforms					561:570	different glycoforms	551:570	different glycoforms	551:570	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	1	66	theme	prefusion	268:276	arg1	conformation					278:289	the prefusion conformation	264:289	the prefusion conformation	264:289	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	3	67	theme	sequence	774:781	arg1	evolution					749:757	the evolution	745:757	the evolution of the protein sequence	745:781	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	4	68	theme	dynamics	856:863	arg1	simulations					865:875	multi-microsecond molecular dynamics simulations	828:875	multi-microsecond molecular dynamics simulations	828:875	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	6	69	theme	host	1585:1588	arg1	localization					1603:1614	host cell surface localization	1585:1614	host cell surface localization	1585:1614	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	5	70	gly	N-glycosylation	1292:1306	arg2	N370					1311:1314	N370	1311:1314	N370	1311:1314	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	5	70	gly	N-glycosylation	1292:1306	arg1	N370					1311:1314	N370	1311:1314	N370	1311:1314	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	6	71	theme	glycan	1483:1488	arg1	cleft					1498:1502	the RBD glycan binding cleft	1475:1502	the RBD glycan binding cleft	1475:1502	We discuss how the absence of the N370 glycan in the SARS-CoV-2 S frees the RBD glycan binding cleft, which becomes available to bind cell-surface glycans, and potentially increases host cell surface localization.					
35126971	2	72	theme	cell	420:423	arg1	nature					401:406	the nature	397:406	the nature of the host cell	397:423	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	2	73	gly	occupancy	325:333	arg2	glycans					370:376	the SARS-CoV-2 S glycans	353:376	the SARS-CoV-2 S glycans	353:376	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	2	74	theme	glycans	370:376	arg1	structures					339:348	structures	339:348	structures	339:348	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	2	74	theme	glycans	370:376	arg1	occupancy					325:333	occupancy	325:333	occupancy	325:333	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	5	75	theme	S	1188:1188	arg1	topology					1206:1213	the SARS-CoV-2 S glycan shield's topology	1173:1213	the SARS-CoV-2 S glycan shield's topology	1173:1213	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	2	76	theme	SARS-CoV-2	357:366	arg1	glycans					370:376	the SARS-CoV-2 S glycans	353:376	the SARS-CoV-2 S glycans	353:376	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	5	77	theme	shield	1197:1202	arg1	topology					1206:1213	the SARS-CoV-2 S glycan shield's topology	1173:1213	the SARS-CoV-2 S glycan shield's topology	1173:1213	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	1	78	theme	immune	224:229	arg1	evasion					231:237	immune evasion	224:237	immune evasion	224:237	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	0	79	dep	nature	35:40	arg1	the					31:33	the	31:33	the	31:33	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	1	80	theme	SARS-CoV-2	182:191	arg1	architecture					203:214	the SARS-CoV-2 spike (S) architecture	178:214	the SARS-CoV-2 spike (S) architecture	178:214	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
35126971	5	81	theme	SARS-CoV-2	1253:1262	arg1	infectivity					1264:1274	the SARS-CoV-2 infectivity	1249:1274	the SARS-CoV-2 infectivity	1249:1274	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	0	82	theme	nature	35:40	arg1	role					23:26	the spike: role	12:26	the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S	12:124	Fine-tuning the spike: role of the nature and topology of the glycan shield in the structure and dynamics of the SARS-CoV-2 S.					
35126971	3	83	dep	glycan	610:615	arg1	shield					617:622	shield	617:622	shield	617:622	Moreover, the functional role of the glycan shield in the SARS-CoV-2 pathogenesis suggests that the evolution of the sites of glycosylation is potentially intertwined with the evolution of the protein sequence to affect optimal activity.					
35126971	5	84	theme	RBD	1390:1392	arg1	surface					1394:1400	the RBD surface	1386:1400	the RBD surface	1386:1400	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	4	85	theme	receptor	977:984	arg1	RBD					1002:1004	RBD	1002:1004	RBD	1002:1004	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	85	theme	receptor	977:984	arg1	domain					994:999	the receptor binding domain	973:999	the receptor binding domain (RBD) open conformation	973:1023	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	86	gly	glycosylation	903:915	arg1	N165					926:929	N165	926:929	N165	926:929	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	86	gly	glycosylation	903:915	arg2	N234					920:923	N234	920:923	N234	920:923	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	86	gly	glycosylation	903:915	arg1	N343					935:938	N343	935:938	N343	935:938	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	86	gly	glycosylation	903:915	arg2	N165					926:929	N165	926:929	N165	926:929	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	86	gly	glycosylation	903:915	arg2	N343					935:938	N343	935:938	N343	935:938	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	4	86	gly	glycosylation	903:915	arg1	N234					920:923	N234	920:923	N234	920:923	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	5	87	theme	glycosylation	1117:1129	arg1	loss					1109:1112	the loss	1105:1112	the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology,	1105:1214	Furthermore, our results suggest that the loss of glycosylation at N370, a newly acquired modification in the SARS-CoV-2 S glycan shield's topology, may have contributed to increase the SARS-CoV-2 infectivity as we find that N-glycosylation at N370 stabilizes the closed RBD conformation by binding a specific cleft on the RBD surface.					
35126971	4	88	theme	domain	994:999	arg1	conformation					1012:1023	the receptor binding domain (RBD) open conformation	973:1023	the receptor binding domain (RBD) open conformation	973:1023	Our results from multi-microsecond molecular dynamics simulations indicate that the type of glycosylation at N234, N165 and N343 greatly affects the stability of the receptor binding domain (RBD) open conformation, and thus its exposure and accessibility.					
35126971	2	89	theme	Recent	292:297	arg1	studies					299:305	Recent studies	292:305	Recent studies	292:305	Recent studies indicate that the occupancy and structures of the SARS-CoV-2 S glycans depend not only on the nature of the host cell, but also on the structural stability of the trimer; a point that raises important questions about the relative competence of different glycoforms.					
35126971	1	90	theme	spike	193:197	arg1	architecture					203:214	the SARS-CoV-2 spike (S) architecture	178:214	the SARS-CoV-2 spike (S) architecture	178:214	The dense glycan shield is an essential feature of the SARS-CoV-2 spike (S) architecture, key to immune evasion and to the activation of the prefusion conformation.					
32442575	0	0	from	characteristics	30:44	arg1	bioactivity					14:24	bioactivity	14:24	bioactivity	14:24	Comparison in bioactivity and characteristics of Ginkgo biloba seed polysaccharides from four extract pathways.					
32442575	6	1	theme	molecular	1193:1201	arg1	weight					1203:1208	molecular weight	1193:1208	molecular weight (4.2 × 105 Da)	1193:1223	The HWE-polysaccharide had the largest molecular weight (4.2 × 105 Da), reduced by the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da).					
32442575	6	1	theme	molecular	1193:1201	arg1	4.2 × 105 Da					1211:1222	4.2 × 105 Da	1211:1222	4.2 × 105 Da	1211:1222	The HWE-polysaccharide had the largest molecular weight (4.2 × 105 Da), reduced by the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da).					
32442575	1	2	theme	ultrasound-enzyme	228:244	arg1	UETE					266:269	UETE	266:269	UETE	266:269	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	2	theme	ultrasound-enzyme	228:244	arg1	extraction					254:263	ultrasound-enzyme treated extraction	228:263	ultrasound-enzyme treated extraction (UETE)	228:270	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	6	3	contain	had	1177:1179	arg1	HWE-polysaccharide					1158:1175	The HWE-polysaccharide	1154:1175	The HWE-polysaccharide	1154:1175	The HWE-polysaccharide had the largest molecular weight (4.2 × 105 Da), reduced by the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da).					
32442575	6	3	contain	had	1177:1179	arg2	4.2 × 105 Da					1211:1222	4.2 × 105 Da	1211:1222	4.2 × 105 Da	1211:1222	The HWE-polysaccharide had the largest molecular weight (4.2 × 105 Da), reduced by the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da).					
32442575	6	3	contain	had	1177:1179	arg2	weight					1203:1208	molecular weight	1193:1208	molecular weight (4.2 × 105 Da)	1193:1223	The HWE-polysaccharide had the largest molecular weight (4.2 × 105 Da), reduced by the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da).					
32442575	5	4	theme	8.25:1.00:1.53	1138:1151	arg1	ratio					1129:1133	a molar ratio	1121:1133	a molar ratio of 8.25:1.00:1.53	1121:1151	The HWE-polysaccharide mainly consisted of homogeneous mannose; the UETE-polysaccharide was primarily composed of mannose, rhamnose, and glucose in a molar ratio of 8.25:1.00:1.53.					
32442575	6	5	theme	largest	1185:1191	arg1	weight					1203:1208	molecular weight	1193:1208	molecular weight (4.2 × 105 Da)	1193:1223	The HWE-polysaccharide had the largest molecular weight (4.2 × 105 Da), reduced by the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da).					
32442575	6	5	theme	largest	1185:1191	arg1	4.2 × 105 Da					1211:1222	4.2 × 105 Da	1211:1222	4.2 × 105 Da	1211:1222	The HWE-polysaccharide had the largest molecular weight (4.2 × 105 Da), reduced by the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da).					
32442575	1	6	theme	treated	246:252	arg1	UETE					266:269	UETE	266:269	UETE	266:269	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	6	theme	treated	246:252	arg1	extraction					254:263	ultrasound-enzyme treated extraction	228:263	ultrasound-enzyme treated extraction (UETE)	228:270	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	0	7	from	Comparison	0:9	arg1	bioactivity					14:24	bioactivity	14:24	bioactivity	14:24	Comparison in bioactivity and characteristics of Ginkgo biloba seed polysaccharides from four extract pathways.					
32442575	8	8	theme	biloba	1521:1526	arg1	seeds					1528:1532	G. biloba seeds	1518:1532	G. biloba seeds	1518:1532	The UETE-polysaccharide from G. biloba seeds showed the highest bioactive activities and distinctive structural characteristics.					
32442575	1	9	theme	extraction	254:263	arg1	effects					116:122	The effects	112:122	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity	112:311	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	9	theme	extraction	254:263	arg1	characteristics					341:355	characteristics	341:355	characteristics of Ginkgo biloba seed polysaccharides	341:393	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	9	theme	extraction	254:263	arg1	activities					326:335	antioxidant activities	314:335	antioxidant activities	314:335	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	0	10	theme	extract	94:100	arg1	pathways					102:109	four extract pathways	89:109	four extract pathways	89:109	Comparison in bioactivity and characteristics of Ginkgo biloba seed polysaccharides from four extract pathways.					
32442575	4	11	theme	monosaccharide	945:958	arg1	composition					960:970	monosaccharide composition	945:970	monosaccharide composition	945:970	The four polysaccharides varied in monosaccharide composition.					
32442575	3	12	dep	ETE-polysaccharide	772:789	arg1	the					768:770	the	768:770	the	768:770	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	2	13	theme	α-glucosidase	539:551	arg1	activity					564:571	the highest α-glucosidase inhibitory activity	527:571	the highest α-glucosidase inhibitory activity	527:571	Among the four extracted polysaccharides, the UETE-polysaccharide initially exhibited the highest α-glucosidase inhibitory activity and antioxidant activities.					
32442575	1	14	theme	Ginkgo	360:365	arg1	polysaccharides					379:393	Ginkgo biloba seed polysaccharides	360:393	Ginkgo biloba seed polysaccharides	360:393	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	6	15	theme	UETE-polysaccharide	1254:1272	arg1	order					1241:1245	the order	1237:1245	the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da)	1237:1364	The HWE-polysaccharide had the largest molecular weight (4.2 × 105 Da), reduced by the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da).					
32442575	5	16	theme	homogeneous	1016:1026	arg1	mannose					1028:1034	homogeneous mannose	1016:1034	homogeneous mannose	1016:1034	The HWE-polysaccharide mainly consisted of homogeneous mannose; the UETE-polysaccharide was primarily composed of mannose, rhamnose, and glucose in a molar ratio of 8.25:1.00:1.53.					
32442575	1	17	theme	ultrasound-treated	155:172	arg1	UTE					186:188	UTE	186:188	UTE	186:188	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	17	theme	ultrasound-treated	155:172	arg1	extraction					174:183	ultrasound-treated extraction	155:183	ultrasound-treated extraction (UTE)	155:189	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	18	from	activities	326:335	arg1	activity					304:311	the α-glucosidase inhibitory activity	275:311	the α-glucosidase inhibitory activity	275:311	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	19	theme	biloba	367:372	arg1	polysaccharides					379:393	Ginkgo biloba seed polysaccharides	360:393	Ginkgo biloba seed polysaccharides	360:393	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	8	20	theme	structural	1590:1599	arg1	characteristics					1601:1615	distinctive structural characteristics	1578:1615	distinctive structural characteristics	1578:1615	The UETE-polysaccharide from G. biloba seeds showed the highest bioactive activities and distinctive structural characteristics.					
32442575	1	21	theme	extraction	174:183	arg1	effects					116:122	The effects	112:122	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity	112:311	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	21	theme	extraction	174:183	arg1	characteristics					341:355	characteristics	341:355	characteristics of Ginkgo biloba seed polysaccharides	341:393	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	21	theme	extraction	174:183	arg1	activities					326:335	antioxidant activities	314:335	antioxidant activities	314:335	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	22	theme	seed	374:377	arg1	polysaccharides					379:393	Ginkgo biloba seed polysaccharides	360:393	Ginkgo biloba seed polysaccharides	360:393	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	3	23	with	spongy	820:825	arg1	topography					847:856	smooth surface topography	832:856	smooth surface topography	832:856	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	7	24	theme	significant	1406:1416	arg1	effects					1418:1424	significant effects	1406:1424	significant effects	1406:1424	Thus, the four extract methods exerted significant effects on the bioactivity and characteristics of the polysaccharides.					
32442575	6	25	theme	UTE-polysaccharide	1331:1348	arg1	order					1241:1245	the order	1237:1245	the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da)	1237:1364	The HWE-polysaccharide had the largest molecular weight (4.2 × 105 Da), reduced by the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da).					
32442575	1	26	from	effects	116:122	arg1	activity					304:311	the α-glucosidase inhibitory activity	275:311	the α-glucosidase inhibitory activity	275:311	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	3	27	theme	pleated	735:741	arg1	shape					750:754	an irregular pleated porous shape	722:754	an irregular pleated porous shape	722:754	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	3	28	theme	smooth	832:837	arg1	topography					847:856	smooth surface topography	832:856	smooth surface topography	832:856	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	7	29	theme	polysaccharides	1472:1486	arg1	characteristics					1449:1463	characteristics	1449:1463	characteristics	1449:1463	Thus, the four extract methods exerted significant effects on the bioactivity and characteristics of the polysaccharides.					
32442575	7	29	theme	polysaccharides	1472:1486	arg1	bioactivity					1433:1443	bioactivity	1433:1443	bioactivity	1433:1443	Thus, the four extract methods exerted significant effects on the bioactivity and characteristics of the polysaccharides.					
32442575	7	30	dep	bioactivity	1433:1443	arg1	the					1429:1431	the	1429:1431	the	1429:1431	Thus, the four extract methods exerted significant effects on the bioactivity and characteristics of the polysaccharides.					
32442575	1	31	theme	polysaccharides	379:393	arg1	effects					116:122	The effects	112:122	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity	112:311	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	31	theme	polysaccharides	379:393	arg1	characteristics					341:355	characteristics	341:355	characteristics of Ginkgo biloba seed polysaccharides	341:393	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	31	theme	polysaccharides	379:393	arg1	activities					326:335	antioxidant activities	314:335	antioxidant activities	314:335	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	8	32	theme	highest	1545:1551	arg1	activities					1563:1572	the highest bioactive activities	1541:1572	the highest bioactive activities	1541:1572	The UETE-polysaccharide from G. biloba seeds showed the highest bioactive activities and distinctive structural characteristics.					
32442575	7	33	theme	extract	1382:1388	arg1	methods					1390:1396	the four extract methods	1373:1396	the four extract methods	1373:1396	Thus, the four extract methods exerted significant effects on the bioactivity and characteristics of the polysaccharides.					
32442575	2	34	theme	antioxidant	577:587	arg1	activities					589:598	antioxidant activities	577:598	antioxidant activities	577:598	Among the four extracted polysaccharides, the UETE-polysaccharide initially exhibited the highest α-glucosidase inhibitory activity and antioxidant activities.					
32442575	8	35	theme	bioactive	1553:1561	arg1	activities					1563:1572	the highest bioactive activities	1541:1572	the highest bioactive activities	1541:1572	The UETE-polysaccharide from G. biloba seeds showed the highest bioactive activities and distinctive structural characteristics.					
32442575	8	36	from	seeds	1528:1532	arg1	UETE-polysaccharide					1493:1511	The UETE-polysaccharide	1489:1511	The UETE-polysaccharide from G. biloba seeds	1489:1532	The UETE-polysaccharide from G. biloba seeds showed the highest bioactive activities and distinctive structural characteristics.					
32442575	1	37	theme	enzyme-treated	192:205	arg1	ETE					219:221	ETE	219:221	ETE	219:221	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	37	theme	enzyme-treated	192:205	arg1	extraction					207:216	enzyme-treated extraction	192:216	enzyme-treated extraction (ETE)	192:222	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	38	from	characteristics	341:355	arg1	activity					304:311	the α-glucosidase inhibitory activity	275:311	the α-glucosidase inhibitory activity	275:311	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	39	theme	α-glucosidase	279:291	arg1	activity					304:311	the α-glucosidase inhibitory activity	275:311	the α-glucosidase inhibitory activity	275:311	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	8	40	theme	distinctive	1578:1588	arg1	characteristics					1601:1615	distinctive structural characteristics	1578:1615	distinctive structural characteristics	1578:1615	The UETE-polysaccharide from G. biloba seeds showed the highest bioactive activities and distinctive structural characteristics.					
32442575	0	41	dep	Ginkgo	49:54	arg1	biloba					56:61	Ginkgo biloba	49:61	Ginkgo biloba	49:61	Comparison in bioactivity and characteristics of Ginkgo biloba seed polysaccharides from four extract pathways.					
32442575	1	42	theme	extraction	207:216	arg1	effects					116:122	The effects	112:122	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity	112:311	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	42	theme	extraction	207:216	arg1	characteristics					341:355	characteristics	341:355	characteristics of Ginkgo biloba seed polysaccharides	341:393	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	42	theme	extraction	207:216	arg1	activities					326:335	antioxidant activities	314:335	antioxidant activities	314:335	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	43	theme	inhibitory	293:302	arg1	activity					304:311	the α-glucosidase inhibitory activity	275:311	the α-glucosidase inhibitory activity	275:311	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	3	44	theme	large	633:637	arg1	number					639:644	a large number	631:644	a large number of small compact spherical structures	631:682	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	0	45	theme	Ginkgo	49:54	arg1	Comparison					0:9	Comparison	0:9	Comparison in bioactivity	0:24	Comparison in bioactivity and characteristics of Ginkgo biloba seed polysaccharides from four extract pathways.					
32442575	0	45	theme	Ginkgo	49:54	arg1	characteristics					30:44	characteristics	30:44	characteristics of Ginkgo biloba	30:61	Comparison in bioactivity and characteristics of Ginkgo biloba seed polysaccharides from four extract pathways.					
32442575	3	46	theme	surface	839:845	arg1	topography					847:856	smooth surface topography	832:856	smooth surface topography	832:856	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	2	47	theme	extracted	456:464	arg1	polysaccharides					466:480	the four extracted polysaccharides	447:480	the four extracted polysaccharides	447:480	Among the four extracted polysaccharides, the UETE-polysaccharide initially exhibited the highest α-glucosidase inhibitory activity and antioxidant activities.					
32442575	5	48	theme	molar	1123:1127	arg1	ratio					1129:1133	a molar ratio	1121:1133	a molar ratio of 8.25:1.00:1.53	1121:1151	The HWE-polysaccharide mainly consisted of homogeneous mannose; the UETE-polysaccharide was primarily composed of mannose, rhamnose, and glucose in a molar ratio of 8.25:1.00:1.53.					
32442575	3	49	theme	porous	743:748	arg1	shape					750:754	an irregular pleated porous shape	722:754	an irregular pleated porous shape	722:754	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	3	50	theme	structures	673:682	arg1	number					639:644	a large number	631:644	a large number of small compact spherical structures	631:682	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	3	51	theme	small	649:653	arg1	structures					673:682	small compact spherical structures	649:682	small compact spherical structures	649:682	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	1	52	theme	hot-water	127:135	arg1	HWE					149:151	HWE	149:151	HWE	149:151	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	52	theme	hot-water	127:135	arg1	extraction					137:146	hot-water extraction	127:146	hot-water extraction (HWE)	127:152	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	2	53	theme	highest	531:537	arg1	activity					564:571	the highest α-glucosidase inhibitory activity	527:571	the highest α-glucosidase inhibitory activity	527:571	Among the four extracted polysaccharides, the UETE-polysaccharide initially exhibited the highest α-glucosidase inhibitory activity and antioxidant activities.					
32442575	3	54	theme	scanning	874:881	arg1	SEM					904:906	SEM	904:906	SEM	904:906	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	3	54	theme	scanning	874:881	arg1	microscopy					892:901	scanning electron microscopy	874:901	scanning electron microscopy (SEM)	874:907	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	0	55	from	pathways	102:109	arg1	polysaccharides					68:82	polysaccharides	68:82	polysaccharides from four extract pathways	68:109	Comparison in bioactivity and characteristics of Ginkgo biloba seed polysaccharides from four extract pathways.					
32442575	6	56	theme	ETE-polysaccharide	1291:1308	arg1	order					1241:1245	the order	1237:1245	the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da)	1237:1364	The HWE-polysaccharide had the largest molecular weight (4.2 × 105 Da), reduced by the order of the UETE-polysaccharide (2.02 × 104 Da), ETE-polysaccharide (1.72 × 104 Da), and UTE-polysaccharide (1.34 × 104 Da).					
32442575	1	57	theme	extraction	137:146	arg1	effects					116:122	The effects	112:122	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity	112:311	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	57	theme	extraction	137:146	arg1	characteristics					341:355	characteristics	341:355	characteristics of Ginkgo biloba seed polysaccharides	341:393	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	1	57	theme	extraction	137:146	arg1	activities					326:335	antioxidant activities	314:335	antioxidant activities	314:335	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	3	58	theme	electron	883:890	arg1	SEM					904:906	SEM	904:906	SEM	904:906	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	3	58	theme	electron	883:890	arg1	microscopy					892:901	scanning electron microscopy	874:901	scanning electron microscopy (SEM)	874:907	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	3	59	theme	irregular	725:733	arg1	shape					750:754	an irregular pleated porous shape	722:754	an irregular pleated porous shape	722:754	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	3	60	theme	compact	655:661	arg1	structures					673:682	small compact spherical structures	649:682	small compact spherical structures	649:682	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	8	61	theme	G.	1518:1519	arg1	seeds					1528:1532	G. biloba seeds	1518:1532	G. biloba seeds	1518:1532	The UETE-polysaccharide from G. biloba seeds showed the highest bioactive activities and distinctive structural characteristics.					
32442575	1	62	theme	antioxidant	314:324	arg1	activities					326:335	antioxidant activities	314:335	antioxidant activities	314:335	The effects of hot-water extraction (HWE), ultrasound-treated extraction (UTE), enzyme-treated extraction (ETE) and ultrasound-enzyme treated extraction (UETE) on the α-glucosidase inhibitory activity, antioxidant activities and characteristics of Ginkgo biloba seed polysaccharides were investigated and compared in this study.					
32442575	3	63	theme	spherical	663:671	arg1	structures					673:682	small compact spherical structures	649:682	small compact spherical structures	649:682	The HWE-polysaccharide showed a large number of small compact spherical structures, and the UTE-polysaccharide exhibited an irregular pleated porous shape; meanwhile, the ETE-polysaccharide and UETE-polysaccharide were spongy with smooth surface topography, as observed by scanning electron microscopy (SEM).					
32442575	2	64	theme	inhibitory	553:562	arg1	activity					564:571	the highest α-glucosidase inhibitory activity	527:571	the highest α-glucosidase inhibitory activity	527:571	Among the four extracted polysaccharides, the UETE-polysaccharide initially exhibited the highest α-glucosidase inhibitory activity and antioxidant activities.					
33229157	5	0	theme	by-products	1055:1065	arg1	chain					1021:1025	the exploitation chain	1004:1025	the exploitation chain of A. mearnsii products and by-products	1004:1065	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	5	1	theme	stabilizing	921:931	arg1	gum					933:935	a potential stabilizing gum	909:935	a potential stabilizing gum for the food industry	909:957	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	5	1	theme	stabilizing	921:931	arg1	gum					819:821	A. mearnsii gum	807:821	A. mearnsii gum	807:821	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	3	2	theme	fibroblast	473:482	arg1	cells					484:488	fibroblast cells	473:488	fibroblast cells (Balb/3T3)	473:499	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	3	2	theme	fibroblast	473:482	arg1	Balb/3T3					491:498	Balb/3T3	491:498	Balb/3T3	491:498	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	0	3	theme	Arabic	53:58	arg1	gum					49:51	an alternative gum	34:51	an alternative gum Arabic for food stabilizer	34:78	Acacia mearnsii gum: A residue as an alternative gum Arabic for food stabilizer.					
33229157	5	4	theme	economic	980:987	arg1	development					989:999	the economic development	976:999	the economic development of the exploitation chain of A. mearnsii products and by-products	976:1065	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	3	5	theme	cells	484:488	arg1	hepatocarcinoma					505:519	hepatocarcinoma	505:519	hepatocarcinoma (HepG2)	505:527	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	3	5	theme	cells	484:488	arg1	proliferation					456:468	proliferation	456:468	proliferation	456:468	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	3	5	theme	cells	484:488	arg1	viability					442:450	viability	442:450	viability	442:450	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	3	5	theme	cells	484:488	arg1	HepG2					522:526	HepG2	522:526	HepG2	522:526	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	5	6	theme	potential	911:919	arg1	gum					933:935	a potential stabilizing gum	909:935	a potential stabilizing gum for the food industry	909:957	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	5	6	theme	potential	911:919	arg1	gum					819:821	A. mearnsii gum	807:821	A. mearnsii gum	807:821	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	2	7	theme	gum	232:234	arg1	stabilizer					315:324	a stabilizer	313:324	a stabilizer in ice cream	313:337	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	7	theme	gum	232:234	arg1	composition					251:261	the A. mearnsii gum polysaccharide composition	216:261	the A. mearnsii gum polysaccharide composition	216:261	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	7	theme	gum	232:234	arg1	cytotoxicity					268:279	its cytotoxicity	264:279	its cytotoxicity	264:279	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	7	theme	gum	232:234	arg1	effect					303:308	the technological effect	285:308	the technological effect	285:308	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	8	theme	ice	329:331	arg1	cream					333:337	ice cream	329:337	ice cream	329:337	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	3	9	dep	viability	442:450	arg1	the					438:440	the	438:440	the	438:440	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	0	10	theme	food	64:67	arg1	stabilizer					69:78	food stabilizer	64:78	food stabilizer	64:78	Acacia mearnsii gum: A residue as an alternative gum Arabic for food stabilizer.					
33229157	4	11	theme	melting	701:707	arg1	characteristics					709:723	the same melting characteristics	692:723	the same melting characteristics as the ice cream samples made with commercial gum Arabic	692:780	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	4	12	theme	more	638:641	arg1	interactions					643:654	more interactions	638:654	a more structured system (more interactions between the mixture components)	612:686	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	5	13	theme	A.	1030:1031	arg1	products					1042:1049	A. mearnsii products	1030:1049	A. mearnsii products	1030:1049	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	2	14	from	stabilizer	315:324	arg1	cream					333:337	ice cream	329:337	ice cream	329:337	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	4	15	theme	ice	732:734	arg1	samples					742:748	the ice cream samples	728:748	the ice cream samples made with commercial gum Arabic	728:780	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	4	16	dep	system	630:635	arg1	interactions					643:654	more interactions	638:654	a more structured system (more interactions between the mixture components)	612:686	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	2	17	dep	A.	220:221	arg1	mearnsii					223:230	mearnsii	223:230	mearnsii	223:230	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	0	18	theme	mearnsii	7:14	arg1	gum					16:18	Acacia mearnsii gum	0:18	Acacia mearnsii gum: A residue as an alternative gum Arabic for food stabilizer.	0:79	Acacia mearnsii gum: A residue as an alternative gum Arabic for food stabilizer.					
33229157	5	19	theme	products	1042:1049	arg1	chain					1021:1025	the exploitation chain	1004:1025	the exploitation chain of A. mearnsii products and by-products	1004:1065	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	5	20	theme	tannin	874:879	arg1	production					881:890	tannin production	874:890	tannin production	874:890	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	3	21	theme	commercial	395:404	arg1	Arabic					410:415	commercial gum Arabic	395:415	commercial gum Arabic	395:415	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	0	22	theme	Acacia	0:5	arg1	gum					16:18	Acacia mearnsii gum	0:18	Acacia mearnsii gum: A residue as an alternative gum Arabic for food stabilizer.	0:79	Acacia mearnsii gum: A residue as an alternative gum Arabic for food stabilizer.					
33229157	2	23	theme	technological	289:301	arg1	stabilizer					315:324	a stabilizer	313:324	a stabilizer in ice cream	313:337	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	23	theme	technological	289:301	arg1	composition					251:261	the A. mearnsii gum polysaccharide composition	216:261	the A. mearnsii gum polysaccharide composition	216:261	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	23	theme	technological	289:301	arg1	cytotoxicity					268:279	its cytotoxicity	264:279	its cytotoxicity	264:279	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	23	theme	technological	289:301	arg1	effect					303:308	the technological effect	285:308	the technological effect	285:308	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	3	24	theme	gum	406:408	arg1	Arabic					410:415	commercial gum Arabic	395:415	commercial gum Arabic	395:415	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	5	25	dep	A.	807:808	arg1	mearnsii					810:817	mearnsii	810:817	mearnsii	810:817	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	4	26	theme	same	696:699	arg1	characteristics					709:723	the same melting characteristics	692:723	the same melting characteristics as the ice cream samples made with commercial gum Arabic	692:780	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	5	27	theme	food	945:948	arg1	industry					950:957	the food industry	941:957	the food industry	941:957	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	1	28	theme	A.	168:169	arg1	cultivation					180:190	A. mearnsii cultivation	168:190	A. mearnsii cultivation	168:190	Acacia mearnsii gum is not commercially exploited, being characterized as residue from A. mearnsii cultivation.					
33229157	4	29	theme	Rheological	530:540	arg1	tests					542:546	Rheological tests	530:546	Rheological tests	530:546	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	1	30	theme	Acacia	81:86	arg1	gum					97:99	Acacia mearnsii gum	81:99	Acacia mearnsii gum	81:99	Acacia mearnsii gum is not commercially exploited, being characterized as residue from A. mearnsii cultivation.					
33229157	4	31	theme	structured	619:628	arg1	system					630:635	a more structured system	612:635	a more structured system (more interactions between the mixture components)	612:686	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	4	32	theme	commercial	760:769	arg1	Arabic					775:780	commercial gum Arabic	760:780	commercial gum Arabic	760:780	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	5	33	from	production	881:890	arg1	residue					861:867	an agro-industrial residue	842:867	an agro-industrial residue from tannin production for industry	842:903	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	5	33	from	production	881:890	arg1	gum					819:821	A. mearnsii gum	807:821	A. mearnsii gum	807:821	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	5	34	theme	exploitation	1008:1019	arg1	chain					1021:1025	the exploitation chain	1004:1025	the exploitation chain of A. mearnsii products and by-products	1004:1065	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	1	35	from	cultivation	180:190	arg1	residue					155:161	residue	155:161	residue from A. mearnsii cultivation	155:190	Acacia mearnsii gum is not commercially exploited, being characterized as residue from A. mearnsii cultivation.					
33229157	3	36	theme	similar	365:371	arg1	structure					382:390	a similar chemical structure	363:390	a similar chemical structure	363:390	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	5	37	theme	chain	1021:1025	arg1	development					989:999	the economic development	976:999	the economic development of the exploitation chain of A. mearnsii products and by-products	976:1065	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	4	38	dep	A.	592:593	arg1	mearnsii					595:602	mearnsii	595:602	mearnsii	595:602	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	1	39	theme	mearnsii	88:95	arg1	gum					97:99	Acacia mearnsii gum	81:99	Acacia mearnsii gum	81:99	Acacia mearnsii gum is not commercially exploited, being characterized as residue from A. mearnsii cultivation.					
33229157	3	40	theme	chemical	373:380	arg1	structure					382:390	a similar chemical structure	363:390	a similar chemical structure	363:390	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	4	41	theme	gum	771:773	arg1	Arabic					775:780	commercial gum Arabic	760:780	commercial gum Arabic	760:780	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	5	42	theme	agro-industrial	845:859	arg1	residue					861:867	an agro-industrial residue	842:867	an agro-industrial residue from tannin production for industry	842:903	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	5	42	theme	agro-industrial	845:859	arg1	gum					819:821	A. mearnsii gum	807:821	A. mearnsii gum	807:821	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	5	43	dep	A.	1030:1031	arg1	mearnsii					1033:1040	mearnsii	1033:1040	mearnsii	1033:1040	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	4	44	theme	cream	736:740	arg1	samples					742:748	the ice cream samples	728:748	the ice cream samples made with commercial gum Arabic	728:780	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	2	45	theme	polysaccharide	236:249	arg1	stabilizer					315:324	a stabilizer	313:324	a stabilizer in ice cream	313:337	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	45	theme	polysaccharide	236:249	arg1	composition					251:261	the A. mearnsii gum polysaccharide composition	216:261	the A. mearnsii gum polysaccharide composition	216:261	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	45	theme	polysaccharide	236:249	arg1	cytotoxicity					268:279	its cytotoxicity	264:279	its cytotoxicity	264:279	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	45	theme	polysaccharide	236:249	arg1	effect					303:308	the technological effect	285:308	the technological effect	285:308	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	3	46	dep	A.	340:341	arg1	mearnsii					343:350	mearnsii	343:350	mearnsii	343:350	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
33229157	4	47	contain	had	608:610	arg1	cream					568:572	the ice cream	560:572	the ice cream stabilized by the A. mearnsii gum	560:606	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	4	47	contain	had	608:610	arg2	characteristics					709:723	the same melting characteristics	692:723	the same melting characteristics as the ice cream samples made with commercial gum Arabic	692:780	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	4	47	contain	had	608:610	arg2	system					630:635	a more structured system	612:635	a more structured system (more interactions between the mixture components)	612:686	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	0	48	dep	gum	16:18	arg1	residue					23:29	A residue	21:29	Acacia mearnsii gum: A residue as an alternative gum Arabic for food stabilizer.	0:79	Acacia mearnsii gum: A residue as an alternative gum Arabic for food stabilizer.					
33229157	4	49	theme	mixture	668:674	arg1	components					676:685	the mixture components	664:685	the mixture components	664:685	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	4	50	theme	ice	564:566	arg1	cream					568:572	the ice cream	560:572	the ice cream stabilized by the A. mearnsii gum	560:606	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	1	51	dep	A.	168:169	arg1	mearnsii					171:178	mearnsii	171:178	mearnsii	171:178	Acacia mearnsii gum is not commercially exploited, being characterized as residue from A. mearnsii cultivation.					
33229157	4	52	theme	A.	592:593	arg1	gum					604:606	the A. mearnsii gum	588:606	the A. mearnsii gum	588:606	Rheological tests showed that the ice cream stabilized by the A. mearnsii gum had a more structured system (more interactions between the mixture components) and the same melting characteristics as the ice cream samples made with commercial gum Arabic.					
33229157	5	53	theme	A.	807:808	arg1	residue					861:867	an agro-industrial residue	842:867	an agro-industrial residue from tannin production for industry	842:903	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	5	53	theme	A.	807:808	arg1	gum					933:935	a potential stabilizing gum	909:935	a potential stabilizing gum for the food industry	909:957	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	5	53	theme	A.	807:808	arg1	gum					819:821	A. mearnsii gum	807:821	A. mearnsii gum	807:821	The results showed that A. mearnsii gum, which is actually an agro-industrial residue from tannin production for industry, is a potential stabilizing gum for the food industry, contributing to the economic development of the exploitation chain of A. mearnsii products and by-products.					
33229157	0	54	theme	alternative	37:47	arg1	gum					49:51	an alternative gum	34:51	an alternative gum Arabic for food stabilizer	34:78	Acacia mearnsii gum: A residue as an alternative gum Arabic for food stabilizer.					
33229157	2	55	theme	A.	220:221	arg1	stabilizer					315:324	a stabilizer	313:324	a stabilizer in ice cream	313:337	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	55	theme	A.	220:221	arg1	composition					251:261	the A. mearnsii gum polysaccharide composition	216:261	the A. mearnsii gum polysaccharide composition	216:261	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	55	theme	A.	220:221	arg1	cytotoxicity					268:279	its cytotoxicity	264:279	its cytotoxicity	264:279	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	2	55	theme	A.	220:221	arg1	effect					303:308	the technological effect	285:308	the technological effect	285:308	This work investigated the A. mearnsii gum polysaccharide composition, its cytotoxicity and the technological effect as a stabilizer in ice cream.					
33229157	3	56	theme	A.	340:341	arg1	gum					352:354	A. mearnsii gum	340:354	A. mearnsii gum	340:354	A. mearnsii gum showed a similar chemical structure to commercial gum Arabic and did not decrease the viability and proliferation of fibroblast cells (Balb/3T3) and hepatocarcinoma (HepG2).					
32358987	9	0	theme	Guggenheim-Anderson-deBoer	1645:1670	arg1	model					1678:1682	the Guggenheim-Anderson-deBoer (GAB) model	1641:1682	the Guggenheim-Anderson-deBoer (GAB) model	1641:1682	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	6	1	theme	noticeable	1059:1068	arg1	decrease					1070:1077	A noticeable decrease	1057:1077	A noticeable decrease in tensile strength of films with increasing concentration	1057:1136	A noticeable decrease in tensile strength of films with increasing concentration was determined, but films prepared with high concentration showed more flexible.					
32358987	10	2	theme	non-biodegradable	1892:1908	arg1	packaging					1918:1926	non-biodegradable plastic packaging	1892:1926	non-biodegradable plastic packaging	1892:1926	The results showed that mulberry pekmez films based alginate have a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging.					
32358987	9	3	theme	GAB	1673:1675	arg1	model					1678:1682	the Guggenheim-Anderson-deBoer (GAB) model	1641:1682	the Guggenheim-Anderson-deBoer (GAB) model	1641:1682	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	1	4	theme	different	239:247	arg1	polysaccharides					249:263	different polysaccharides	239:263	different polysaccharides	239:263	Production of composite films is an important approach to improve functionality of edible films, by combining different polysaccharides, proteins, and lipids.					
32358987	1	5	theme	films	153:157	arg1	approach					175:182	an important approach	162:182	an important approach to improve functionality of edible films	162:223	Production of composite films is an important approach to improve functionality of edible films, by combining different polysaccharides, proteins, and lipids.					
32358987	1	5	theme	films	153:157	arg1	Production					129:138	Production	129:138	Production of composite films	129:157	Production of composite films is an important approach to improve functionality of edible films, by combining different polysaccharides, proteins, and lipids.					
32358987	6	6	theme	increasing	1113:1122	arg1	concentration					1124:1136	increasing concentration	1113:1136	increasing concentration	1113:1136	A noticeable decrease in tensile strength of films with increasing concentration was determined, but films prepared with high concentration showed more flexible.					
32358987	5	7	theme	R2	1044:1045	arg1	model					1037:1041	Ostwald de Waele model	1020:1041	Ostwald de Waele model (R2 ≥ 0.943)	1020:1054	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	5	7	theme	R2	1044:1045	arg1	≥ 0.943					1047:1053	R2 ≥ 0.943	1044:1053	R2 ≥ 0.943	1044:1053	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	3	8	theme	efficient	557:565	arg1	properties					575:584	efficient barrier properties	557:584	efficient barrier properties	557:584	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	8	9	theme	reverse	1455:1461	arg1	behavior					1463:1470	reverse behavior	1455:1470	reverse behavior	1455:1470	Increasing pekmez concentration in the film matrix improved the water vapor permeability of alginate films, whereas pectin films showed reverse behavior as resulting in cracks and crack propagation within the structure.					
32358987	5	10	theme	de	1028:1029	arg1	model					1037:1041	Ostwald de Waele model	1020:1041	Ostwald de Waele model (R2 ≥ 0.943)	1020:1054	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	5	10	theme	de	1028:1029	arg1	≥ 0.943					1047:1053	R2 ≥ 0.943	1044:1053	R2 ≥ 0.943	1044:1053	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	5	11	theme	film-forming	891:902	arg1	solutions					904:912	All film-forming solutions	887:912	All film-forming solutions	887:912	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	9	12	theme	typical	1580:1586	arg1	profile					1588:1594	a typical profile	1578:1594	a typical profile of foods	1578:1603	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	2	13	theme	colloidal	401:409	arg1	properties					411:420	their unique colloidal properties	388:420	their unique colloidal properties	388:420	Carbohydrate-based composite films are most attractive, which have good film-forming ability due to their unique colloidal properties.					
32358987	0	14	theme	nigra	58:62	arg1	molasses					73:80	molasses	73:80	molasses	73:80	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	0	14	theme	nigra	58:62	arg1	films					93:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	3	15	theme	oxygen	622:627	arg1	transmission					629:640	oxygen transmission	622:640	oxygen transmission	622:640	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	9	16	dep	showed	1571:1576	arg1	contain					1605:1611	contain	1605:1611	showed a typical profile of foods contain high soluble components	1571:1635	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	0	17	theme	pekmez	65:70	arg1	molasses					73:80	molasses	73:80	molasses	73:80	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	0	17	theme	pekmez	65:70	arg1	films					93:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	4	18	theme	composite	762:770	arg1	films					772:776	composite films	762:776	composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin	762:884	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	5	19	theme	higher	955:960	arg1	stress					968:973	higher yield stress	955:973	higher yield stress	955:973	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	8	20	theme	film	1358:1361	arg1	matrix					1363:1368	the film matrix	1354:1368	the film matrix	1354:1368	Increasing pekmez concentration in the film matrix improved the water vapor permeability of alginate films, whereas pectin films showed reverse behavior as resulting in cracks and crack propagation within the structure.					
32358987	3	21	theme	Fruit	423:427	arg1	purees					429:434	Fruit purees	423:434	Fruit purees	423:434	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	0	22	theme	composite	83:91	arg1	molasses					73:80	molasses	73:80	molasses	73:80	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	0	22	theme	composite	83:91	arg1	films					93:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	9	23	theme	films	1565:1569	arg1	isotherms					1552:1560	The sorption isotherms	1539:1560	The sorption isotherms of films	1539:1569	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	4	24	theme	water	733:737	arg1	properties					748:757	physical, barrier, mechanical, thermal, and water sorption properties	689:757	properties	748:757	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	2	25	theme	film-forming	360:371	arg1	ability					373:379	good film-forming ability	355:379	good film-forming ability	355:379	Carbohydrate-based composite films are most attractive, which have good film-forming ability due to their unique colloidal properties.					
32358987	5	26	theme	shear	926:930	arg1	behavior					941:948	shear thinning behavior	926:948	shear thinning behavior	926:948	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	8	27	theme	water	1383:1387	arg1	permeability					1395:1406	the water vapor permeability	1379:1406	the water vapor permeability of alginate films	1379:1424	Increasing pekmez concentration in the film matrix improved the water vapor permeability of alginate films, whereas pectin films showed reverse behavior as resulting in cracks and crack propagation within the structure.					
32358987	3	28	theme	polysaccharide	449:462	arg1	content					464:470	high polysaccharide content	444:470	high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission	444:640	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	7	29	theme	alginate	1303:1310	arg1	films					1312:1316	alginate films	1303:1316	alginate films	1303:1316	The mechanical properties of pectin films exhibited weakened properties compared to alginate films.					
32358987	10	30	theme	physical	1845:1852	arg1	properties					1854:1863	the physical properties	1841:1863	the physical properties	1841:1863	The results showed that mulberry pekmez films based alginate have a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging.					
32358987	0	31	theme	black	36:40	arg1	molasses					73:80	molasses	73:80	molasses	73:80	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	0	31	theme	black	36:40	arg1	films					93:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	9	32	theme	foods	1599:1603	arg1	profile					1588:1594	a typical profile	1578:1594	a typical profile of foods	1578:1603	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	8	33	theme	alginate	1411:1418	arg1	films					1420:1424	alginate films	1411:1424	alginate films	1411:1424	Increasing pekmez concentration in the film matrix improved the water vapor permeability of alginate films, whereas pectin films showed reverse behavior as resulting in cracks and crack propagation within the structure.					
32358987	9	34	theme	high	1613:1616	arg1	components					1626:1635	high soluble components	1613:1635	high soluble components	1613:1635	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	7	35	theme	weakened	1271:1278	arg1	properties					1280:1289	weakened properties	1271:1289	weakened properties	1271:1289	The mechanical properties of pectin films exhibited weakened properties compared to alginate films.					
32358987	4	36	theme	different	794:802	arg1	°Brix					851:855	26, 32, and 38 °Brix	836:855	26, 32, and 38 °Brix	836:855	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	4	36	theme	different	794:802	arg1	concentrations					820:833	different mulberry pekmez concentrations	794:833	different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin	794:884	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	5	37	theme	viscosity-shear	979:993	arg1	data					1000:1003	viscosity-shear rate data	979:1003	viscosity-shear rate data	979:1003	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	7	38	theme	films	1255:1259	arg1	properties					1234:1243	The mechanical properties	1219:1243	The mechanical properties of pectin films	1219:1259	The mechanical properties of pectin films exhibited weakened properties compared to alginate films.					
32358987	8	39	theme	pectin	1435:1440	arg1	films					1442:1446	pectin films	1435:1446	pectin films	1435:1446	Increasing pekmez concentration in the film matrix improved the water vapor permeability of alginate films, whereas pectin films showed reverse behavior as resulting in cracks and crack propagation within the structure.					
32358987	3	40	theme	desirable	498:506	arg1	permeability					601:612	selective permeability	591:612	selective permeability	591:612	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	3	40	theme	desirable	498:506	arg1	properties					513:522	desirable film properties	498:522	desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability	498:612	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	3	40	theme	desirable	498:506	arg1	resistance					545:554	mechanical resistance	534:554	mechanical resistance	534:554	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	3	40	theme	desirable	498:506	arg1	properties					575:584	efficient barrier properties	557:584	efficient barrier properties	557:584	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	1	41	theme	edible	212:217	arg1	films					219:223	edible films	212:223	edible films	212:223	Production of composite films is an important approach to improve functionality of edible films, by combining different polysaccharides, proteins, and lipids.					
32358987	10	42	theme	plastic	1910:1916	arg1	packaging					1918:1926	non-biodegradable plastic packaging	1892:1926	non-biodegradable plastic packaging	1892:1926	The results showed that mulberry pekmez films based alginate have a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging.					
32358987	6	43	from	decrease	1070:1077	arg1	strength					1090:1097	tensile strength	1082:1097	tensile strength of films with increasing concentration	1082:1136	A noticeable decrease in tensile strength of films with increasing concentration was determined, but films prepared with high concentration showed more flexible.					
32358987	6	44	with	films	1102:1106	arg1	concentration					1124:1136	increasing concentration	1113:1136	increasing concentration	1113:1136	A noticeable decrease in tensile strength of films with increasing concentration was determined, but films prepared with high concentration showed more flexible.					
32358987	7	45	theme	mechanical	1223:1232	arg1	properties					1234:1243	The mechanical properties	1219:1243	The mechanical properties of pectin films	1219:1259	The mechanical properties of pectin films exhibited weakened properties compared to alginate films.					
32358987	3	46	theme	mechanical	534:543	arg1	resistance					545:554	mechanical resistance	534:554	mechanical resistance	534:554	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	2	47	theme	Carbohydrate-based	288:305	arg1	films					317:321	Carbohydrate-based composite films	288:321	Carbohydrate-based composite films	288:321	Carbohydrate-based composite films are most attractive, which have good film-forming ability due to their unique colloidal properties.					
32358987	8	48	theme	pekmez	1330:1335	arg1	concentration					1337:1349	pekmez concentration	1330:1349	pekmez concentration	1330:1349	Increasing pekmez concentration in the film matrix improved the water vapor permeability of alginate films, whereas pectin films showed reverse behavior as resulting in cracks and crack propagation within the structure.					
32358987	1	49	theme	composite	143:151	arg1	films					153:157	composite films	143:157	composite films	143:157	Production of composite films is an important approach to improve functionality of edible films, by combining different polysaccharides, proteins, and lipids.					
32358987	4	50	theme	study	663:667	arg1	purpose					647:653	The purpose	643:653	The purpose of this study	643:667	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	3	51	theme	barrier	567:573	arg1	properties					575:584	efficient barrier properties	557:584	efficient barrier properties	557:584	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	8	52	dep	improved	1370:1377	arg1	whereas					1427:1433	whereas	1427:1433	whereas	1427:1433	Increasing pekmez concentration in the film matrix improved the water vapor permeability of alginate films, whereas pectin films showed reverse behavior as resulting in cracks and crack propagation within the structure.					
32358987	9	53	theme	good	1691:1694	arg1	fit					1696:1698	a good fit	1689:1698	a good fit for all of the obtained data	1689:1727	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	10	54	dep	applications	1817:1828	arg1	depends					1830:1836	depends	1830:1836	depends on the physical properties	1830:1863	The results showed that mulberry pekmez films based alginate have a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging.					
32358987	5	55	theme	Ostwald	1020:1026	arg1	model					1037:1041	Ostwald de Waele model	1020:1041	Ostwald de Waele model (R2 ≥ 0.943)	1020:1054	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	5	55	theme	Ostwald	1020:1026	arg1	≥ 0.943					1047:1053	R2 ≥ 0.943	1044:1053	R2 ≥ 0.943	1044:1053	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	10	56	theme	pekmez	1763:1768	arg1	films					1770:1774	mulberry pekmez films	1754:1774	mulberry pekmez films based alginate	1754:1789	The results showed that mulberry pekmez films based alginate have a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging.					
32358987	1	57	theme	important	165:173	arg1	approach					175:182	an important approach	162:182	an important approach to improve functionality of edible films	162:223	Production of composite films is an important approach to improve functionality of edible films, by combining different polysaccharides, proteins, and lipids.					
32358987	1	57	theme	important	165:173	arg1	Production					129:138	Production	129:138	Production of composite films	129:157	Production of composite films is an important approach to improve functionality of edible films, by combining different polysaccharides, proteins, and lipids.					
32358987	3	58	theme	selective	591:599	arg1	permeability					601:612	selective permeability	591:612	selective permeability	591:612	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	9	59	theme	obtained	1715:1722	arg1	data					1724:1727	the obtained data	1711:1727	the obtained data	1711:1727	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	4	60	theme	mechanical	708:717	arg1	barrier					699:705	physical, barrier, mechanical, thermal, and water sorption properties	689:757	barrier	699:705	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	5	61	theme	Waele	1031:1035	arg1	model					1037:1041	Ostwald de Waele model	1020:1041	Ostwald de Waele model (R2 ≥ 0.943)	1020:1054	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	5	61	theme	Waele	1031:1035	arg1	≥ 0.943					1047:1053	R2 ≥ 0.943	1044:1053	R2 ≥ 0.943	1044:1053	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	6	62	theme	high	1178:1181	arg1	concentration					1183:1195	high concentration	1178:1195	high concentration	1178:1195	A noticeable decrease in tensile strength of films with increasing concentration was determined, but films prepared with high concentration showed more flexible.					
32358987	4	63	theme	thermal	720:726	arg1	barrier					699:705	physical, barrier, mechanical, thermal, and water sorption properties	689:757	barrier	699:705	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	10	64	theme	food	1812:1815	arg1	applications					1817:1828	food applications	1812:1828	food applications depends on the physical properties	1812:1863	The results showed that mulberry pekmez films based alginate have a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging.					
32358987	8	65	theme	crack	1499:1503	arg1	propagation					1505:1515	crack propagation	1499:1515	crack propagation	1499:1515	Increasing pekmez concentration in the film matrix improved the water vapor permeability of alginate films, whereas pectin films showed reverse behavior as resulting in cracks and crack propagation within the structure.					
32358987	4	66	theme	physical	689:696	arg1	barrier					699:705	physical, barrier, mechanical, thermal, and water sorption properties	689:757	barrier	699:705	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	9	67	theme	sorption	1543:1550	arg1	isotherms					1552:1560	The sorption isotherms	1539:1560	The sorption isotherms of films	1539:1569	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	10	68	theme	mulberry	1754:1761	arg1	films					1770:1774	mulberry pekmez films	1754:1774	mulberry pekmez films based alginate	1754:1789	The results showed that mulberry pekmez films based alginate have a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging.					
32358987	6	69	theme	tensile	1082:1088	arg1	strength					1090:1097	tensile strength	1082:1097	tensile strength of films with increasing concentration	1082:1136	A noticeable decrease in tensile strength of films with increasing concentration was determined, but films prepared with high concentration showed more flexible.					
32358987	0	70	theme	films	93:97	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	0	70	theme	films	93:97	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	5	71	theme	yield	962:966	arg1	stress					968:973	higher yield stress	955:973	higher yield stress	955:973	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	4	72	theme	films	772:776	arg1	barrier					699:705	physical, barrier, mechanical, thermal, and water sorption properties	689:757	barrier	699:705	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	4	72	theme	films	772:776	arg1	properties					748:757	physical, barrier, mechanical, thermal, and water sorption properties	689:757	properties	748:757	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	2	73	contain	have	350:353	arg1	attractive					332:341	attractive	332:341	attractive	332:341	Carbohydrate-based composite films are most attractive, which have good film-forming ability due to their unique colloidal properties.					
32358987	2	73	contain	have	350:353	arg2	ability					373:379	good film-forming ability	355:379	good film-forming ability	355:379	Carbohydrate-based composite films are most attractive, which have good film-forming ability due to their unique colloidal properties.					
32358987	10	74	contain	have	1791:1794	arg2	potential					1798:1806	a potential	1796:1806	a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging	1796:1926	The results showed that mulberry pekmez films based alginate have a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging.					
32358987	10	74	contain	have	1791:1794	arg1	films					1770:1774	mulberry pekmez films	1754:1774	mulberry pekmez films based alginate	1754:1789	The results showed that mulberry pekmez films based alginate have a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging.					
32358987	2	75	theme	good	355:358	arg1	ability					373:379	good film-forming ability	355:379	good film-forming ability	355:379	Carbohydrate-based composite films are most attractive, which have good film-forming ability due to their unique colloidal properties.					
32358987	4	76	theme	pekmez	813:818	arg1	°Brix					851:855	26, 32, and 38 °Brix	836:855	26, 32, and 38 °Brix	836:855	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	4	76	theme	pekmez	813:818	arg1	concentrations					820:833	different mulberry pekmez concentrations	794:833	different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin	794:884	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	5	77	theme	thinning	932:939	arg1	behavior					941:948	shear thinning behavior	926:948	shear thinning behavior	926:948	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	4	78	theme	sorption	739:746	arg1	properties					748:757	physical, barrier, mechanical, thermal, and water sorption properties	689:757	properties	748:757	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	2	79	theme	unique	394:399	arg1	properties					411:420	their unique colloidal properties	388:420	their unique colloidal properties	388:420	Carbohydrate-based composite films are most attractive, which have good film-forming ability due to their unique colloidal properties.					
32358987	0	80	theme	mulberry	42:49	arg1	molasses					73:80	molasses	73:80	molasses	73:80	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	0	80	theme	mulberry	42:49	arg1	films					93:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	8	81	theme	vapor	1389:1393	arg1	permeability					1395:1406	the water vapor permeability	1379:1406	the water vapor permeability of alginate films	1379:1424	Increasing pekmez concentration in the film matrix improved the water vapor permeability of alginate films, whereas pectin films showed reverse behavior as resulting in cracks and crack propagation within the structure.					
32358987	3	82	theme	high	444:447	arg1	content					464:470	high polysaccharide content	444:470	high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission	444:640	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	0	83	theme	Morus	52:56	arg1	molasses					73:80	molasses	73:80	molasses	73:80	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	0	83	theme	Morus	52:56	arg1	films					93:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	black mulberry (Morus nigra) pekmez (molasses) composite films	36:97	Development and characterization of black mulberry (Morus nigra) pekmez (molasses) composite films based on alginate and pectin.					
32358987	6	84	theme	films	1102:1106	arg1	strength					1090:1097	tensile strength	1082:1097	tensile strength of films with increasing concentration	1082:1136	A noticeable decrease in tensile strength of films with increasing concentration was determined, but films prepared with high concentration showed more flexible.					
32358987	9	85	theme	soluble	1618:1624	arg1	components					1626:1635	high soluble components	1613:1635	high soluble components	1613:1635	The sorption isotherms of films showed a typical profile of foods contain high soluble components and the Guggenheim-Anderson-deBoer (GAB) model gave a good fit for all of the obtained data.					
32358987	7	86	theme	pectin	1248:1253	arg1	films					1255:1259	pectin films	1248:1259	pectin films	1248:1259	The mechanical properties of pectin films exhibited weakened properties compared to alginate films.					
32358987	8	87	theme	films	1420:1424	arg1	permeability					1395:1406	the water vapor permeability	1379:1406	the water vapor permeability of alginate films	1379:1424	Increasing pekmez concentration in the film matrix improved the water vapor permeability of alginate films, whereas pectin films showed reverse behavior as resulting in cracks and crack propagation within the structure.					
32358987	2	88	theme	composite	307:315	arg1	films					317:321	Carbohydrate-based composite films	288:321	Carbohydrate-based composite films	288:321	Carbohydrate-based composite films are most attractive, which have good film-forming ability due to their unique colloidal properties.					
32358987	5	89	theme	rate	995:998	arg1	data					1000:1003	viscosity-shear rate data	979:1003	viscosity-shear rate data	979:1003	All film-forming solutions were showed shear thinning behavior with higher yield stress and viscosity-shear rate data were fitted to Ostwald de Waele model (R2 ≥ 0.943).					
32358987	10	90	theme	packaging	1918:1926	arg1	replacement					1877:1887	the replacement	1873:1887	the replacement of non-biodegradable plastic packaging	1873:1926	The results showed that mulberry pekmez films based alginate have a potential for food applications depends on the physical properties and for the replacement of non-biodegradable plastic packaging.					
32358987	4	91	theme	mulberry	804:811	arg1	°Brix					851:855	26, 32, and 38 °Brix	836:855	26, 32, and 38 °Brix	836:855	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	4	91	theme	mulberry	804:811	arg1	concentrations					820:833	different mulberry pekmez concentrations	794:833	different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin	794:884	The purpose of this study was to characterize physical, barrier, mechanical, thermal, and water sorption properties of composite films formulated with different mulberry pekmez concentrations (26, 32, and 38 °Brix) based on alginate or pectin.					
32358987	3	92	theme	film	508:511	arg1	permeability					601:612	selective permeability	591:612	selective permeability	591:612	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	3	92	theme	film	508:511	arg1	properties					513:522	desirable film properties	498:522	desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability	498:612	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	3	92	theme	film	508:511	arg1	resistance					545:554	mechanical resistance	534:554	mechanical resistance	534:554	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	3	92	theme	film	508:511	arg1	properties					575:584	efficient barrier properties	557:584	efficient barrier properties	557:584	Fruit purees include high polysaccharide content that plays a role to have desirable film properties including mechanical resistance, efficient barrier properties, and selective permeability against oxygen transmission.					
32358987	1	93	theme	films	219:223	arg1	functionality					195:207	functionality	195:207	functionality of edible films	195:223	Production of composite films is an important approach to improve functionality of edible films, by combining different polysaccharides, proteins, and lipids.					
34864594	0	0	theme	1H	77:78	arg1	spectroscopy					84:95	1H NMR spectroscopy	77:95	1H NMR spectroscopy	77:95	Quality control and product differentiation of LMWHs marketed in China using 1H NMR spectroscopy and chemometric tools.					
34864594	3	1	theme	LMWHs	519:523	arg1	safety					509:514	safety	509:514	safety	509:514	The Chinese drug regulatory authorities have been committed to improve the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market.					
34864594	3	1	theme	LMWHs	519:523	arg1	quality					497:503	quality	497:503	quality	497:503	The Chinese drug regulatory authorities have been committed to improve the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market.					
34864594	1	2	theme	lengths	246:252	arg1	mixture					225:231	mixture	225:231	mixture of different lengths and substitution patterns	225:278	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	3	3	theme	Chinese	372:378	arg1	authorities					396:406	The Chinese drug regulatory authorities	368:406	The Chinese drug regulatory authorities	368:406	The Chinese drug regulatory authorities have been committed to improve the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market.					
34864594	3	4	theme	current	528:534	arg1	market					544:549	current Chinese market	528:549	current Chinese market	528:549	The Chinese drug regulatory authorities have been committed to improve the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market.					
34864594	3	5	dep	quality	497:503	arg1	the					493:495	the	493:495	the	493:495	The Chinese drug regulatory authorities have been committed to improve the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market.					
34864594	1	6	theme	substitution	258:269	arg1	patterns					271:278	substitution patterns	258:278	substitution patterns	258:278	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	3	7	theme	drug	380:383	arg1	authorities					396:406	The Chinese drug regulatory authorities	368:406	The Chinese drug regulatory authorities	368:406	The Chinese drug regulatory authorities have been committed to improve the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market.					
34864594	5	8	theme	manufacture	884:894	arg1	consistency					864:874	the batch-to-batch consistency	845:874	the batch-to-batch consistency of each manufacture	845:894	The method can be used not only to monitor impurities and contaminants, but also to check the batch-to-batch consistency of each manufacture.					
34864594	1	9	theme	patterns	271:278	arg1	mixture					225:231	mixture	225:231	mixture of different lengths and substitution patterns	225:278	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	0	10	theme	chemometric	101:111	arg1	tools					113:117	chemometric tools	101:117	chemometric tools	101:117	Quality control and product differentiation of LMWHs marketed in China using 1H NMR spectroscopy and chemometric tools.					
34864594	4	11	theme	chemometric	733:743	arg1	analysis					745:752	chemometric analysis	733:752	chemometric analysis	733:752	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	2	12	theme	Structural	281:290	arg1	characterization					292:307	Structural characterization	281:307	Structural characterization	281:307	Structural characterization and quality control of LMWHs have always been challenging.					
34864594	1	13	theme	heterogeneous	162:174	arg1	heparins					141:148	Low molecular weight heparins	120:148	Low molecular weight heparins (LMWHs)	120:156	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	1	13	theme	heterogeneous	162:174	arg1	mixtures					176:183	heterogeneous mixtures	162:183	heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns	162:278	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	3	14	theme	LMWHs	468:472	arg1	standards					455:463	the supervision standards	439:463	the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market	439:549	The Chinese drug regulatory authorities have been committed to improve the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market.					
34864594	2	15	theme	LMWHs	332:336	arg1	characterization					292:307	Structural characterization	281:307	Structural characterization	281:307	Structural characterization and quality control of LMWHs have always been challenging.					
34864594	2	15	theme	LMWHs	332:336	arg1	control					321:327	quality control	313:327	quality control	313:327	Structural characterization and quality control of LMWHs have always been challenging.					
34864594	3	16	theme	supervision	443:453	arg1	standards					455:463	the supervision standards	439:463	the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market	439:549	The Chinese drug regulatory authorities have been committed to improve the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market.					
34864594	0	17	theme	Quality	0:6	arg1	control					8:14	Quality control	0:14	Quality control	0:14	Quality control and product differentiation of LMWHs marketed in China using 1H NMR spectroscopy and chemometric tools.					
34864594	4	18	theme	NMR	713:715	arg1	experiments					717:727	1H NMR experiments	710:727	1H NMR experiments	710:727	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	5	19	theme	each	879:882	arg1	manufacture					884:894	each manufacture	879:894	each manufacture	879:894	The method can be used not only to monitor impurities and contaminants, but also to check the batch-to-batch consistency of each manufacture.					
34864594	0	20	theme	product	20:26	arg1	differentiation					28:42	product differentiation	20:42	product differentiation	20:42	Quality control and product differentiation of LMWHs marketed in China using 1H NMR spectroscopy and chemometric tools.					
34864594	4	21	theme	present	559:565	arg1	paper					567:571	the present paper	555:571	the present paper	555:571	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	7	22	dep	quality	1126:1132	arg1	the					1122:1124	the	1122:1124	the	1122:1124	By using this method, the quality and safety of LMWHs marketed in China were initially assessed.					
34864594	6	23	from	manufactures	947:958	arg1	LMWHs					926:930	the biosimilar LMWHs	911:930	the biosimilar LMWHs from different manufactures	911:958	Moreover, for the biosimilar LMWHs from different manufactures, they can be differentiated and clustered according to their slightly different structural compositions originated from production process.					
34864594	2	24	theme	quality	313:319	arg1	control					321:327	quality control	313:327	quality control	313:327	Structural characterization and quality control of LMWHs have always been challenging.					
34864594	1	25	theme	glycosaminoglycan	188:204	arg1	chains					206:211	glycosaminoglycan chains	188:211	glycosaminoglycan chains composed of mixture of different lengths and substitution patterns	188:278	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	7	26	theme	LMWHs	1148:1152	arg1	safety					1138:1143	safety	1138:1143	safety	1138:1143	By using this method, the quality and safety of LMWHs marketed in China were initially assessed.					
34864594	7	26	theme	LMWHs	1148:1152	arg1	quality					1126:1132	quality	1126:1132	quality	1126:1132	By using this method, the quality and safety of LMWHs marketed in China were initially assessed.					
34864594	6	27	theme	structural	1040:1049	arg1	compositions					1051:1062	their slightly different structural compositions	1015:1062	their slightly different structural compositions originated from production process	1015:1097	Moreover, for the biosimilar LMWHs from different manufactures, they can be differentiated and clustered according to their slightly different structural compositions originated from production process.					
34864594	1	28	theme	chains	206:211	arg1	heparins					141:148	Low molecular weight heparins	120:148	Low molecular weight heparins (LMWHs)	120:156	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	1	28	theme	chains	206:211	arg1	mixtures					176:183	heterogeneous mixtures	162:183	heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns	162:278	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	6	29	theme	different	937:945	arg1	manufactures					947:958	different manufactures	937:958	different manufactures	937:958	Moreover, for the biosimilar LMWHs from different manufactures, they can be differentiated and clustered according to their slightly different structural compositions originated from production process.					
34864594	5	30	used	used	773:776	arg2	method					759:764	The method	755:764	The method	755:764	The method can be used not only to monitor impurities and contaminants, but also to check the batch-to-batch consistency of each manufacture.					
34864594	3	31	theme	regulatory	385:394	arg1	authorities					396:406	The Chinese drug regulatory authorities	368:406	The Chinese drug regulatory authorities	368:406	The Chinese drug regulatory authorities have been committed to improve the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market.					
34864594	6	32	theme	production	1080:1089	arg1	process					1091:1097	production process	1080:1097	production process	1080:1097	Moreover, for the biosimilar LMWHs from different manufactures, they can be differentiated and clustered according to their slightly different structural compositions originated from production process.					
34864594	5	33	theme	batch-to-batch	849:862	arg1	consistency					864:874	the batch-to-batch consistency	845:874	the batch-to-batch consistency of each manufacture	845:894	The method can be used not only to monitor impurities and contaminants, but also to check the batch-to-batch consistency of each manufacture.					
34864594	6	34	theme	different	1030:1038	arg1	compositions					1051:1062	their slightly different structural compositions	1015:1062	their slightly different structural compositions originated from production process	1015:1097	Moreover, for the biosimilar LMWHs from different manufactures, they can be differentiated and clustered according to their slightly different structural compositions originated from production process.					
34864594	4	35	theme	1H	710:711	arg1	experiments					717:727	1H NMR experiments	710:727	1H NMR experiments	710:727	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	0	36	theme	LMWHs	47:51	arg1	control					8:14	Quality control	0:14	Quality control	0:14	Quality control and product differentiation of LMWHs marketed in China using 1H NMR spectroscopy and chemometric tools.					
34864594	0	36	theme	LMWHs	47:51	arg1	differentiation					28:42	product differentiation	20:42	product differentiation	20:42	Quality control and product differentiation of LMWHs marketed in China using 1H NMR spectroscopy and chemometric tools.					
34864594	6	37	theme	biosimilar	915:924	arg1	LMWHs					926:930	the biosimilar LMWHs	911:930	the biosimilar LMWHs from different manufactures	911:958	Moreover, for the biosimilar LMWHs from different manufactures, they can be differentiated and clustered according to their slightly different structural compositions originated from production process.					
34864594	4	38	theme	different	670:678	arg1	manufacturers					680:692	different manufacturers	670:692	different manufacturers	670:692	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	1	39	theme	Low	120:122	arg1	heparins					141:148	Low molecular weight heparins	120:148	Low molecular weight heparins (LMWHs)	120:156	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	1	39	theme	Low	120:122	arg1	mixtures					176:183	heterogeneous mixtures	162:183	heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns	162:278	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	1	39	theme	Low	120:122	arg1	LMWHs					151:155	LMWHs	151:155	LMWHs	151:155	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	1	40	theme	molecular	124:132	arg1	heparins					141:148	Low molecular weight heparins	120:148	Low molecular weight heparins (LMWHs)	120:156	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	1	40	theme	molecular	124:132	arg1	mixtures					176:183	heterogeneous mixtures	162:183	heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns	162:278	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	1	40	theme	molecular	124:132	arg1	LMWHs					151:155	LMWHs	151:155	LMWHs	151:155	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	4	41	theme	LMWHs	600:604	arg1	batches					577:583	80 batches	574:583	80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers	574:692	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	4	42	dep	LMWHs	600:604	arg1	naldroparin					634:644	naldroparin	634:644	naldroparin	634:644	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	4	42	dep	LMWHs	600:604	arg1	enoxaparin					619:628	enoxaparin	619:628	enoxaparin	619:628	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	4	42	dep	LMWHs	600:604	arg1	dalteparin					607:616	dalteparin	607:616	dalteparin	607:616	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	4	42	dep	LMWHs	600:604	arg1	LMWHs					600:604	three types LMWHs	588:604	three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers	588:692	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	1	43	theme	weight	134:139	arg1	heparins					141:148	Low molecular weight heparins	120:148	Low molecular weight heparins (LMWHs)	120:156	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	1	43	theme	weight	134:139	arg1	mixtures					176:183	heterogeneous mixtures	162:183	heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns	162:278	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	1	43	theme	weight	134:139	arg1	LMWHs					151:155	LMWHs	151:155	LMWHs	151:155	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	4	44	theme	types	594:598	arg1	naldroparin					634:644	naldroparin	634:644	naldroparin	634:644	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	4	44	theme	types	594:598	arg1	enoxaparin					619:628	enoxaparin	619:628	enoxaparin	619:628	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	4	44	theme	types	594:598	arg1	dalteparin					607:616	dalteparin	607:616	dalteparin	607:616	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	4	44	theme	types	594:598	arg1	LMWHs					600:604	three types LMWHs	588:604	three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers	588:692	In the present paper, 80 batches of three types LMWHs (dalteparin, enoxaparin and naldroparin) marketed in China from different manufacturers were studied by 1H NMR experiments and chemometric analysis.					
34864594	1	45	theme	different	236:244	arg1	lengths					246:252	different lengths	236:252	different lengths	236:252	Low molecular weight heparins (LMWHs) are heterogeneous mixtures of glycosaminoglycan chains composed of mixture of different lengths and substitution patterns.					
34864594	3	46	theme	Chinese	536:542	arg1	market					544:549	current Chinese market	528:549	current Chinese market	528:549	The Chinese drug regulatory authorities have been committed to improve the supervision standards of LMWHs to better regulate the quality and safety of LMWHs in current Chinese market.					
34864594	0	47	theme	NMR	80:82	arg1	spectroscopy					84:95	1H NMR spectroscopy	77:95	1H NMR spectroscopy	77:95	Quality control and product differentiation of LMWHs marketed in China using 1H NMR spectroscopy and chemometric tools.					
32504785	9	0	theme	multiple	1670:1677	arg1	technique					1691:1699	the multiple fingerprint technique	1666:1699	the multiple fingerprint technique	1666:1699	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	9	0	theme	multiple	1670:1677	arg1	reliable					1705:1712	reliable	1705:1712	reliable	1705:1712	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	9	1	theme	polysaccharide-enriched	1916:1938	arg1	medicines					1947:1955	polysaccharide-enriched herbal medicines	1916:1955	polysaccharide-enriched herbal medicines	1916:1955	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	1	2	theme	traditional	241:251	arg1	medicine					268:275	a famous traditional Chinese patent medicine	232:275	a famous traditional Chinese patent medicine	232:275	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	1	2	theme	traditional	241:251	arg1	RELEVANCE					196:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP)	175:229	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	9	3	theme	medicines	1947:1955	arg1	quality					1905:1911	the quality	1901:1911	the quality of polysaccharide-enriched herbal medicines	1901:1955	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	1	4	theme	patent	261:266	arg1	medicine					268:275	a famous traditional Chinese patent medicine	232:275	a famous traditional Chinese patent medicine	232:275	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	1	4	theme	patent	261:266	arg1	RELEVANCE					196:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP)	175:229	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	7	5	theme	coefficient	1415:1425	arg1	values					1427:1432	the correlation coefficient values	1399:1432	the correlation coefficient values of multi-fingerprints	1399:1454	RESULTS The similarity evaluation of ZYPPs indicated ZYPPs from different batches showed a high similarity based on the correlation coefficient values of multi-fingerprints.					
32504785	4	6	theme	STUDY	619:623	arg1	AIM					608:610	AIM	608:610	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system	608:741	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	6	7	theme	anti-glycation	1224:1237	arg1	activities					1239:1248	anti-glycation activities	1224:1248	anti-glycation activities	1224:1248	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	3	8	theme	complicated	502:512	arg1	structure					514:522	the complicated structure	498:522	the complicated structure of ZYPPs	498:531	However, the complex compositions of ZYP and the complicated structure of ZYPPs have posed great challenges and barriers to the quality evaluation of ZYP.					
32504785	6	9	theme	antioxidant	1208:1218	arg1	structure					1123:1131	structure	1123:1131	structure	1123:1131	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	6	9	theme	antioxidant	1208:1218	arg1	activity					1148:1155	biological activity	1137:1155	biological activity	1137:1155	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	3	10	theme	great	544:548	arg1	challenges					550:559	great challenges	544:559	great challenges	544:559	However, the complex compositions of ZYP and the complicated structure of ZYPPs have posed great challenges and barriers to the quality evaluation of ZYP.					
32504785	0	11	theme	drugs	132:136	arg1	profiling					21:29	Multiple fingerprint profiling	0:29	Multiple fingerprint profiling for quality evaluation of polysaccharides	0:71	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	0	11	theme	drugs	132:136	arg1	activity					96:103	related biological activity	77:103	related biological activity	77:103	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	1	12	theme	Yutai	213:217	arg1	ZYP					226:228	ZYP	226:228	ZYP	226:228	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	1	12	theme	Yutai	213:217	arg1	Pills					219:223	Zishen Yutai Pills	206:223	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP)	175:229	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	7	13	theme	high	1374:1377	arg1	similarity					1379:1388	a high similarity	1372:1388	a high similarity based on the correlation coefficient values of multi-fingerprints	1372:1454	RESULTS The similarity evaluation of ZYPPs indicated ZYPPs from different batches showed a high similarity based on the correlation coefficient values of multi-fingerprints.					
32504785	9	14	theme	control	1759:1765	arg1	improvement					1736:1746	the improvement	1732:1746	the improvement of quality control of ZYPPs	1732:1774	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	4	15	theme	better	668:673	arg1	control					683:689	better quality control	668:689	better quality control of ZYP, a reliable and valid quality control system	668:741	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	9	16	theme	ZYPPs	1770:1774	arg1	control					1759:1765	quality control	1751:1765	quality control of ZYPPs	1751:1774	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	6	17	theme	chemical	1173:1180	arg1	characteristics					1182:1196	their chemical characteristics	1167:1196	their chemical characteristics	1167:1196	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	7	18	theme	different	1347:1355	arg1	batches					1357:1363	different batches	1347:1363	different batches	1347:1363	RESULTS The similarity evaluation of ZYPPs indicated ZYPPs from different batches showed a high similarity based on the correlation coefficient values of multi-fingerprints.					
32504785	8	19	theme	antioxidant	1497:1507	arg1	properties					1527:1536	remarkable antioxidant and antiglycation properties	1486:1536	remarkable antioxidant and antiglycation properties	1486:1536	Furthermore, ZYPPs exhibited remarkable antioxidant and antiglycation properties, which might be attributed to their molecular weights and the content of uronic acids.					
32504785	1	20	theme	recurrent	311:319	arg1	miscarriage					321:331	recurrent miscarriage	311:331	recurrent miscarriage	311:331	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	3	21	theme	quality	581:587	arg1	evaluation					589:598	the quality evaluation	577:598	the quality evaluation of ZYP	577:605	However, the complex compositions of ZYP and the complicated structure of ZYPPs have posed great challenges and barriers to the quality evaluation of ZYP.					
32504785	9	22	theme	multiple	1792:1799	arg1	technique					1813:1821	the multiple fingerprint technique	1788:1821	the multiple fingerprint technique	1788:1821	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	9	22	theme	multiple	1792:1799	arg1	strategy					1881:1888	a valid and feasible strategy	1860:1888	a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines	1860:1955	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	8	23	theme	antiglycation	1513:1525	arg1	properties					1527:1536	remarkable antioxidant and antiglycation properties	1486:1536	remarkable antioxidant and antiglycation properties	1486:1536	Furthermore, ZYPPs exhibited remarkable antioxidant and antiglycation properties, which might be attributed to their molecular weights and the content of uronic acids.					
32504785	0	24	theme	biological	85:94	arg1	activity					96:103	related biological activity	77:103	related biological activity	77:103	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	5	25	dep	HPLC	869:872	arg1	fingerprint					933:943	partial acid digested fingerprint	911:943	partial acid digested fingerprint	911:943	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	5	25	dep	HPLC	869:872	arg1	fingerprint					898:908	complete acid digested fingerprint	875:908	complete acid digested fingerprint	875:908	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	5	25	dep	HPLC	869:872	arg1	fingerprint					972:982	enzymatically digested fingerprint	949:982	enzymatically digested fingerprint	949:982	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	5	26	theme	ZYPPs	1056:1060	arg1	structure					1043:1051	the chemical structure	1030:1051	the chemical structure of ZYPPs	1030:1060	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	5	27	theme	partial	911:917	arg1	fingerprint					933:943	partial acid digested fingerprint	911:943	partial acid digested fingerprint	911:943	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	4	28	theme	quality	720:726	arg1	system					736:741	a reliable and valid quality control system	699:741	a reliable and valid quality control system	699:741	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	4	28	theme	quality	720:726	arg1	ZYP					694:696	ZYP	694:696	ZYP	694:696	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	0	29	theme	Chinese	117:123	arg1	drugs					132:136	Chinese patent drugs	117:136	Chinese patent drugs	117:136	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	9	30	dep	CONCLUSIONS	1625:1635	arg1	indicated					1651:1659	indicated	1651:1659	indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines	1651:1955	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	5	31	theme	digested	924:931	arg1	fingerprint					933:943	partial acid digested fingerprint	911:943	partial acid digested fingerprint	911:943	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	2	32	theme	theraprutic	425:435	arg1	effects					437:443	the theraprutic effects	421:443	the theraprutic effects of ZYP	421:450	Polysaccharides of ZYP (ZYPPs) play an essential role in the theraprutic effects of ZYP.					
32504785	4	33	theme	ZYP	694:696	arg1	control					683:689	better quality control	668:689	better quality control of ZYP, a reliable and valid quality control system	668:741	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	2	34	theme	ZYP	383:385	arg1	Polysaccharides					364:378	Polysaccharides	364:378	Polysaccharides of ZYP (ZYPPs)	364:393	Polysaccharides of ZYP (ZYPPs) play an essential role in the theraprutic effects of ZYP.					
32504785	0	35	theme	Multiple	0:7	arg1	profiling					21:29	Multiple fingerprint profiling	0:29	Multiple fingerprint profiling for quality evaluation of polysaccharides	0:71	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	5	36	theme	acid	884:887	arg1	fingerprint					898:908	complete acid digested fingerprint	875:908	complete acid digested fingerprint	875:908	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	2	37	theme	essential	403:411	arg1	role					413:416	an essential role	400:416	an essential role	400:416	Polysaccharides of ZYP (ZYPPs) play an essential role in the theraprutic effects of ZYP.					
32504785	6	38	theme	biological	1137:1146	arg1	activity					1148:1155	biological activity	1137:1155	biological activity	1137:1155	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	7	39	theme	similarity	1295:1304	arg1	evaluation					1306:1315	The similarity evaluation	1291:1315	The similarity evaluation of ZYPPs indicated ZYPPs from different batches	1291:1363	RESULTS The similarity evaluation of ZYPPs indicated ZYPPs from different batches showed a high similarity based on the correlation coefficient values of multi-fingerprints.					
32504785	9	40	theme	valid	1862:1866	arg1	technique					1813:1821	the multiple fingerprint technique	1788:1821	the multiple fingerprint technique	1788:1821	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	9	40	theme	valid	1862:1866	arg1	strategy					1881:1888	a valid and feasible strategy	1860:1888	a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines	1860:1955	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	0	41	theme	quality	35:41	arg1	evaluation					43:52	quality evaluation	35:52	quality evaluation of polysaccharides	35:71	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	9	42	theme	feasible	1872:1879	arg1	technique					1813:1821	the multiple fingerprint technique	1788:1821	the multiple fingerprint technique	1788:1821	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	9	42	theme	feasible	1872:1879	arg1	strategy					1881:1888	a valid and feasible strategy	1860:1888	a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines	1860:1955	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	7	43	dep	RESULTS	1283:1289	arg1	showed					1365:1370	showed	1365:1370	showed a high similarity based on the correlation coefficient values of multi-fingerprints	1365:1454	RESULTS The similarity evaluation of ZYPPs indicated ZYPPs from different batches showed a high similarity based on the correlation coefficient values of multi-fingerprints.					
32504785	8	44	theme	acids	1618:1622	arg1	weights					1584:1590	their molecular weights	1568:1590	their molecular weights	1568:1590	Furthermore, ZYPPs exhibited remarkable antioxidant and antiglycation properties, which might be attributed to their molecular weights and the content of uronic acids.					
32504785	8	44	theme	acids	1618:1622	arg1	content					1600:1606	the content	1596:1606	the content of uronic acids	1596:1622	Furthermore, ZYPPs exhibited remarkable antioxidant and antiglycation properties, which might be attributed to their molecular weights and the content of uronic acids.					
32504785	5	45	dep	MATERIALS	774:782	arg1	profile					816:822	A multi-fingerprint profile	796:822	A multi-fingerprint profile	796:822	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	3	46	theme	ZYP	490:492	arg1	structure					514:522	the complicated structure	498:522	the complicated structure of ZYPPs	498:531	However, the complex compositions of ZYP and the complicated structure of ZYPPs have posed great challenges and barriers to the quality evaluation of ZYP.					
32504785	3	46	theme	ZYP	490:492	arg1	compositions					474:485	the complex compositions	462:485	the complex compositions of ZYP	462:492	However, the complex compositions of ZYP and the complicated structure of ZYPPs have posed great challenges and barriers to the quality evaluation of ZYP.					
32504785	5	47	theme	digested	963:970	arg1	fingerprint					972:982	enzymatically digested fingerprint	949:982	enzymatically digested fingerprint	949:982	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	1	48	theme	famous	234:239	arg1	medicine					268:275	a famous traditional Chinese patent medicine	232:275	a famous traditional Chinese patent medicine	232:275	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	1	48	theme	famous	234:239	arg1	RELEVANCE					196:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP)	175:229	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	9	49	theme	fingerprint	1679:1689	arg1	technique					1691:1699	the multiple fingerprint technique	1666:1699	the multiple fingerprint technique	1666:1699	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	9	49	theme	fingerprint	1679:1689	arg1	reliable					1705:1712	reliable	1705:1712	reliable	1705:1712	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	9	50	theme	herbal	1940:1945	arg1	medicines					1947:1955	polysaccharide-enriched herbal medicines	1916:1955	polysaccharide-enriched herbal medicines	1916:1955	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	1	51	theme	Chinese	253:259	arg1	medicine					268:275	a famous traditional Chinese patent medicine	232:275	a famous traditional Chinese patent medicine	232:275	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	1	51	theme	Chinese	253:259	arg1	RELEVANCE					196:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP)	175:229	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	7	52	theme	multi-fingerprints	1437:1454	arg1	values					1427:1432	the correlation coefficient values	1399:1432	the correlation coefficient values of multi-fingerprints	1399:1454	RESULTS The similarity evaluation of ZYPPs indicated ZYPPs from different batches showed a high similarity based on the correlation coefficient values of multi-fingerprints.					
32504785	6	53	theme	activities	1239:1248	arg1	structure					1123:1131	structure	1123:1131	structure	1123:1131	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	6	53	theme	activities	1239:1248	arg1	activity					1148:1155	biological activity	1137:1155	biological activity	1137:1155	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	5	54	theme	multi-fingerprint	798:814	arg1	profile					816:822	A multi-fingerprint profile	796:822	A multi-fingerprint profile	796:822	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	1	55	theme	ETHNOPHARMACOLOGICAL	175:194	arg1	medicine					268:275	a famous traditional Chinese patent medicine	232:275	a famous traditional Chinese patent medicine	232:275	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	1	55	theme	ETHNOPHARMACOLOGICAL	175:194	arg1	RELEVANCE					196:204	ETHNOPHARMACOLOGICAL RELEVANCE	175:204	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP)	175:229	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	7	56	theme	correlation	1403:1413	arg1	values					1427:1432	the correlation coefficient values	1399:1432	the correlation coefficient values of multi-fingerprints	1399:1454	RESULTS The similarity evaluation of ZYPPs indicated ZYPPs from different batches showed a high similarity based on the correlation coefficient values of multi-fingerprints.					
32504785	3	57	theme	ZYPPs	527:531	arg1	structure					514:522	the complicated structure	498:522	the complicated structure of ZYPPs	498:531	However, the complex compositions of ZYP and the complicated structure of ZYPPs have posed great challenges and barriers to the quality evaluation of ZYP.					
32504785	3	57	theme	ZYPPs	527:531	arg1	compositions					474:485	the complex compositions	462:485	the complex compositions of ZYP	462:492	However, the complex compositions of ZYP and the complicated structure of ZYPPs have posed great challenges and barriers to the quality evaluation of ZYP.					
32504785	5	58	theme	MATERIALS	774:782	arg1	strategy					824:831	MATERIALS AND METHODS A multi-fingerprint profile strategy	774:831	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint)	774:983	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	0	59	theme	Yutai	146:150	arg1	Pills					152:156	Zishen Yutai Pills	139:156	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.	0:173	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	5	60	theme	chemical	1034:1041	arg1	structure					1043:1051	the chemical structure	1030:1051	the chemical structure of ZYPPs	1030:1060	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	5	61	theme	METHODS	788:794	arg1	strategy					824:831	MATERIALS AND METHODS A multi-fingerprint profile strategy	774:831	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint)	774:983	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	9	62	theme	quality	1751:1757	arg1	control					1759:1765	quality control	1751:1765	quality control of ZYPPs	1751:1774	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	6	63	theme	in	1199:1200	arg1	antioxidant					1208:1218	in vitro antioxidant	1199:1218	in vitro antioxidant	1199:1218	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	8	64	theme	remarkable	1486:1495	arg1	properties					1527:1536	remarkable antioxidant and antiglycation properties	1486:1536	remarkable antioxidant and antiglycation properties	1486:1536	Furthermore, ZYPPs exhibited remarkable antioxidant and antiglycation properties, which might be attributed to their molecular weights and the content of uronic acids.					
32504785	6	65	theme	characteristics	1182:1196	arg1	structure					1123:1131	structure	1123:1131	structure	1123:1131	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	6	65	theme	characteristics	1182:1196	arg1	activity					1148:1155	biological activity	1137:1155	biological activity	1137:1155	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	0	66	theme	case	163:166	arg1	study					168:172	a case study	161:172	a case study	161:172	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	4	67	theme	quality	675:681	arg1	control					683:689	better quality control	668:689	better quality control of ZYP, a reliable and valid quality control system	668:741	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	0	68	theme	related	77:83	arg1	activity					96:103	related biological activity	77:103	related biological activity	77:103	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	1	69	theme	Zishen	206:211	arg1	ZYP					226:228	ZYP	226:228	ZYP	226:228	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	1	69	theme	Zishen	206:211	arg1	Pills					219:223	Zishen Yutai Pills	206:223	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP)	175:229	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	6	70	theme	ZYPPs	1160:1164	arg1	structure					1123:1131	structure	1123:1131	structure	1123:1131	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	6	70	theme	ZYPPs	1160:1164	arg1	activity					1148:1155	biological activity	1137:1155	biological activity	1137:1155	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	1	71	theme	threatened	343:352	arg1	abortion					354:361	threatened abortion	343:361	threatened abortion	343:361	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	9	72	theme	fingerprint	1801:1811	arg1	technique					1813:1821	the multiple fingerprint technique	1788:1821	the multiple fingerprint technique	1788:1821	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	9	72	theme	fingerprint	1801:1811	arg1	strategy					1881:1888	a valid and feasible strategy	1860:1888	a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines	1860:1955	CONCLUSIONS These results indicated that the multiple fingerprint technique was reliable and effective for the improvement of quality control of ZYPPs, suggesting the multiple fingerprint technique could also be potentially applied as a valid and feasible strategy to control the quality of polysaccharide-enriched herbal medicines.					
32504785	6	73	dep	in	1199:1200	arg1	vitro					1202:1206	vitro	1202:1206	vitro	1202:1206	Besides, the purpose of revealing the relationships between structure and biological activity of ZYPPs, their chemical characteristics, in vitro antioxidant and anti-glycation activities were investigated and discussed.					
32504785	0	74	dep	profiling	21:29	arg1	analysis					105:112	analysis	105:112	analysis	105:112	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	0	74	dep	profiling	21:29	arg1	Pills					152:156	Zishen Yutai Pills	139:156	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.	0:173	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	4	75	theme	reliable	701:708	arg1	system					736:741	a reliable and valid quality control system	699:741	a reliable and valid quality control system	699:741	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	4	75	theme	reliable	701:708	arg1	ZYP					694:696	ZYP	694:696	ZYP	694:696	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	0	76	theme	patent	125:130	arg1	drugs					132:136	Chinese patent drugs	117:136	Chinese patent drugs	117:136	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	5	77	theme	acid	919:922	arg1	fingerprint					933:943	partial acid digested fingerprint	911:943	partial acid digested fingerprint	911:943	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	7	78	theme	ZYPPs	1320:1324	arg1	evaluation					1306:1315	The similarity evaluation	1291:1315	The similarity evaluation of ZYPPs indicated ZYPPs from different batches	1291:1363	RESULTS The similarity evaluation of ZYPPs indicated ZYPPs from different batches showed a high similarity based on the correlation coefficient values of multi-fingerprints.					
32504785	4	79	theme	valid	714:718	arg1	system					736:741	a reliable and valid quality control system	699:741	a reliable and valid quality control system	699:741	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	4	79	theme	valid	714:718	arg1	ZYP					694:696	ZYP	694:696	ZYP	694:696	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	0	80	theme	fingerprint	9:19	arg1	profiling					21:29	Multiple fingerprint profiling	0:29	Multiple fingerprint profiling for quality evaluation of polysaccharides	0:71	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	5	81	theme	complete	875:882	arg1	fingerprint					898:908	complete acid digested fingerprint	875:908	complete acid digested fingerprint	875:908	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	7	82	from	batches	1357:1363	arg1	ZYPPs					1336:1340	ZYPPs	1336:1340	ZYPPs from different batches	1336:1363	RESULTS The similarity evaluation of ZYPPs indicated ZYPPs from different batches showed a high similarity based on the correlation coefficient values of multi-fingerprints.					
32504785	8	83	theme	molecular	1574:1582	arg1	weights					1584:1590	their molecular weights	1568:1590	their molecular weights	1568:1590	Furthermore, ZYPPs exhibited remarkable antioxidant and antiglycation properties, which might be attributed to their molecular weights and the content of uronic acids.					
32504785	5	84	theme	digested	889:896	arg1	fingerprint					898:908	complete acid digested fingerprint	875:908	complete acid digested fingerprint	875:908	MATERIALS AND METHODS A multi-fingerprint profile strategy based on HPSEC-MALL-RID, FT-IR, and HPLC (complete acid digested fingerprint, partial acid digested fingerprint and enzymatically digested fingerprint) was established to identify and discriminate the chemical structure of ZYPPs.					
32504785	2	85	theme	ZYP	448:450	arg1	effects					437:443	the theraprutic effects	421:443	the theraprutic effects of ZYP	421:450	Polysaccharides of ZYP (ZYPPs) play an essential role in the theraprutic effects of ZYP.					
32504785	3	86	theme	ZYP	603:605	arg1	evaluation					589:598	the quality evaluation	577:598	the quality evaluation of ZYP	577:605	However, the complex compositions of ZYP and the complicated structure of ZYPPs have posed great challenges and barriers to the quality evaluation of ZYP.					
32504785	1	87	dep	RELEVANCE	196:204	arg1	ZYP					226:228	ZYP	226:228	ZYP	226:228	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	1	87	dep	RELEVANCE	196:204	arg1	Pills					219:223	Zishen Yutai Pills	206:223	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP)	175:229	ETHNOPHARMACOLOGICAL RELEVANCE Zishen Yutai Pills (ZYP), a famous traditional Chinese patent medicine, has been widely applied to avoid recurrent miscarriage and treat threatened abortion.					
32504785	0	88	theme	polysaccharides	57:71	arg1	evaluation					43:52	quality evaluation	35:52	quality evaluation of polysaccharides	35:71	Multiple fingerprint profiling for quality evaluation of polysaccharides and related biological activity analysis of Chinese patent drugs: Zishen Yutai Pills as a case study.					
32504785	8	89	theme	uronic	1611:1616	arg1	acids					1618:1622	uronic acids	1611:1622	uronic acids	1611:1622	Furthermore, ZYPPs exhibited remarkable antioxidant and antiglycation properties, which might be attributed to their molecular weights and the content of uronic acids.					
32504785	3	90	theme	complex	466:472	arg1	compositions					474:485	the complex compositions	462:485	the complex compositions of ZYP	462:492	However, the complex compositions of ZYP and the complicated structure of ZYPPs have posed great challenges and barriers to the quality evaluation of ZYP.					
32504785	4	91	theme	control	728:734	arg1	system					736:741	a reliable and valid quality control system	699:741	a reliable and valid quality control system	699:741	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32504785	4	91	theme	control	728:734	arg1	ZYP					694:696	ZYP	694:696	ZYP	694:696	AIM OF THE STUDY To identify and characterize the ZYPPs for better quality control of ZYP, a reliable and valid quality control system was established in this study.					
32172840	0	0	theme	polysaccharide	82:95	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	0	0	theme	polysaccharide	82:95	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	0	0	theme	polysaccharide	82:95	arg1	activity					59:66	antioxidant activity	47:66	antioxidant activity	47:66	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	5	1	theme	COP-W1	743:748	arg1	backbone					731:738	the backbone	727:738	the backbone of COP-W1	727:748	The results indicated that the backbone of COP-W1 was composed of (1→3)-linked-galactose, (1→3, 6)-linked-mannose, (1→4)-linked-mannose, and (1→6)-linked-mannose residues, which branched at O-3.					
32172840	5	2	dep	residues	862:869	arg1	1→6					842:844	1→6	842:844	1→6	842:844	The results indicated that the backbone of COP-W1 was composed of (1→3)-linked-galactose, (1→3, 6)-linked-mannose, (1→4)-linked-mannose, and (1→6)-linked-mannose residues, which branched at O-3.					
32172840	4	3	dep	Fourier	559:565	arg1	transform					567:575	transform	567:575	transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis	567:697	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	1	4	theme	ethanol	249:255	arg1	precipitation					257:269	ethanol precipitation	249:269	ethanol precipitation	249:269	A new polysaccharide named COP-W1 was extracted from the root of Codonopsis tangshen Oliv using hot water extraction and ethanol precipitation.					
32172840	0	5	theme	COP-W1	75:80	arg1	polysaccharide					82:95	the COP-W1 polysaccharide	71:95	the COP-W1 polysaccharide from Codonopsis	71:111	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	7	6	theme	scavenging	1148:1157	arg1	activity					1159:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1096:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL)	1096:1228	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	7	7	contain	has	1257:1259	arg2	potential					1265:1273	the potential to be used as a natural antioxidant	1261:1309	the potential to be used as a natural antioxidant	1261:1309	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	7	7	contain	has	1257:1259	arg1	COP-W1					1250:1255	COP-W1	1250:1255	COP-W1	1250:1255	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	0	8	from	Purification	0:11	arg1	Codonopsis					102:111	Codonopsis	102:111	Codonopsis	102:111	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	2	9	theme	molecular	287:295	arg1	weight					297:302	a molecular weight	285:302	a molecular weight of 2.34 × 104 Da	285:319	COP-W1, with a molecular weight of 2.34 × 104 Da, was purified using gel permeation chromatography.					
32172840	7	10	theme	half	1169:1172	arg1	IC50					1208:1211	IC50	1208:1211	IC50	1208:1211	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	7	10	theme	half	1169:1172	arg1	concentration					1193:1205	half maximal inhibition concentration	1169:1205	half maximal inhibition concentration	1169:1205	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	0	11	from	activity	59:66	arg1	Codonopsis					102:111	Codonopsis	102:111	Codonopsis	102:111	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	5	12	dep	-linked-galactose	771:787	arg1	1→3					767:769	1→3	767:769	1→3	767:769	The results indicated that the backbone of COP-W1 was composed of (1→3)-linked-galactose, (1→3, 6)-linked-mannose, (1→4)-linked-mannose, and (1→6)-linked-mannose residues, which branched at O-3.					
32172840	7	13	theme	radical	1140:1146	arg1	activity					1159:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1096:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL)	1096:1228	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	5	14	theme	-linked-mannose	846:860	arg1	residues					862:869	-linked-mannose residues	846:869	-linked-mannose residues	846:869	The results indicated that the backbone of COP-W1 was composed of (1→3)-linked-galactose, (1→3, 6)-linked-mannose, (1→4)-linked-mannose, and (1→6)-linked-mannose residues, which branched at O-3.					
32172840	4	15	theme	chromatograph-mass	612:629	arg1	GC-MS					645:649	GC-MS	645:649	GC-MS	645:649	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	4	15	theme	chromatograph-mass	612:629	arg1	spectrometry					631:642	gas chromatograph-mass spectrometry	608:642	gas chromatograph-mass spectrometry (GC-MS)	608:650	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	7	16	theme	natural	1291:1297	arg1	antioxidant					1299:1309	a natural antioxidant	1289:1309	a natural antioxidant	1289:1309	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	1	17	theme	water	228:232	arg1	extraction					234:243	hot water extraction	224:243	hot water extraction	224:243	A new polysaccharide named COP-W1 was extracted from the root of Codonopsis tangshen Oliv using hot water extraction and ethanol precipitation.					
32172840	4	18	theme	COP-W1	535:540	arg1	characterization					515:530	Structural characterization	504:530	Structural characterization of COP-W1	504:540	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	6	19	dep	-linked-galactose	953:969	arg1	1→6					949:951	1→6	949:951	1→6	949:951	The branches consisted of (1→2, 6)-linked-galactose, (1→6)-linked-galactose, and (1→4)-linked-galactose, each terminating with α-galactose.					
32172840	7	20	theme	maximal	1174:1180	arg1	IC50					1208:1211	IC50	1208:1211	IC50	1208:1211	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	7	20	theme	maximal	1174:1180	arg1	concentration					1193:1205	half maximal inhibition concentration	1169:1205	half maximal inhibition concentration	1169:1205	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	4	21	theme	nuclear	657:663	arg1	NMR					685:687	NMR	685:687	NMR	685:687	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	4	21	theme	nuclear	657:663	arg1	resonance					674:682	nuclear magnetic resonance	657:682	nuclear magnetic resonance (NMR) analysis	657:697	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	3	22	theme	monosaccharide	376:389	arg1	mannose					417:423	mannose	417:423	mannose	417:423	The monosaccharide composition of COP-W1 was mannose, rhamnose, glucose, and galactose, and its molar ratio was 20.32:1:1.27:36.13.					
32172840	3	22	theme	monosaccharide	376:389	arg1	composition					391:401	The monosaccharide composition	372:401	The monosaccharide composition of COP-W1	372:411	The monosaccharide composition of COP-W1 was mannose, rhamnose, glucose, and galactose, and its molar ratio was 20.32:1:1.27:36.13.					
32172840	7	23	dep	activity	1159:1166	arg1	IC50					1208:1211	IC50	1208:1211	IC50	1208:1211	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	7	23	dep	activity	1159:1166	arg1	concentration					1193:1205	half maximal inhibition concentration	1169:1205	half maximal inhibition concentration	1169:1205	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	1	24	theme	Codonopsis	193:202	arg1	Oliv					213:216	Codonopsis tangshen Oliv	193:216	Codonopsis tangshen Oliv	193:216	A new polysaccharide named COP-W1 was extracted from the root of Codonopsis tangshen Oliv using hot water extraction and ethanol precipitation.					
32172840	0	25	theme	structural	14:23	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	5	26	dep	-linked-mannose	820:834	arg1	1→4					816:818	1→4	816:818	1→4	816:818	The results indicated that the backbone of COP-W1 was composed of (1→3)-linked-galactose, (1→3, 6)-linked-mannose, (1→4)-linked-mannose, and (1→6)-linked-mannose residues, which branched at O-3.					
32172840	1	27	theme	tangshen	204:211	arg1	Oliv					213:216	Codonopsis tangshen Oliv	193:216	Codonopsis tangshen Oliv	193:216	A new polysaccharide named COP-W1 was extracted from the root of Codonopsis tangshen Oliv using hot water extraction and ethanol precipitation.					
32172840	3	28	theme	COP-W1	406:411	arg1	mannose					417:423	mannose	417:423	mannose	417:423	The monosaccharide composition of COP-W1 was mannose, rhamnose, glucose, and galactose, and its molar ratio was 20.32:1:1.27:36.13.					
32172840	3	28	theme	COP-W1	406:411	arg1	composition					391:401	The monosaccharide composition	372:401	The monosaccharide composition of COP-W1	372:411	The monosaccharide composition of COP-W1 was mannose, rhamnose, glucose, and galactose, and its molar ratio was 20.32:1:1.27:36.13.					
32172840	1	29	theme	Oliv	213:216	arg1	root					185:188	the root	181:188	the root of Codonopsis tangshen Oliv	181:216	A new polysaccharide named COP-W1 was extracted from the root of Codonopsis tangshen Oliv using hot water extraction and ethanol precipitation.					
32172840	4	30	theme	resonance	674:682	arg1	analysis					690:697	nuclear magnetic resonance (NMR) analysis	657:697	nuclear magnetic resonance (NMR) analysis	657:697	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	4	31	theme	Structural	504:513	arg1	characterization					515:530	Structural characterization	504:530	Structural characterization of COP-W1	504:540	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	4	32	dep	transform	567:575	arg1	infrared					577:584	infrared	577:584	transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis	567:697	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	6	33	dep	-linked-galactose	981:997	arg1	1→4					977:979	1→4	977:979	1→4	977:979	The branches consisted of (1→2, 6)-linked-galactose, (1→6)-linked-galactose, and (1→4)-linked-galactose, each terminating with α-galactose.					
32172840	7	34	theme	inhibition	1182:1191	arg1	IC50					1208:1211	IC50	1208:1211	IC50	1208:1211	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	7	34	theme	inhibition	1182:1191	arg1	concentration					1193:1205	half maximal inhibition concentration	1169:1205	half maximal inhibition concentration	1169:1205	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	0	35	theme	antioxidant	47:57	arg1	activity					59:66	antioxidant activity	47:66	antioxidant activity	47:66	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	2	36	with	COP-W1	272:277	arg1	weight					297:302	a molecular weight	285:302	a molecular weight of 2.34 × 104 Da	285:319	COP-W1, with a molecular weight of 2.34 × 104 Da, was purified using gel permeation chromatography.					
32172840	3	37	theme	molar	468:472	arg1	ratio					474:478	its molar ratio	464:478	its molar ratio	464:478	The monosaccharide composition of COP-W1 was mannose, rhamnose, glucose, and galactose, and its molar ratio was 20.32:1:1.27:36.13.					
32172840	7	38	contain	has	1092:1094	arg1	COP-W1					1085:1090	COP-W1	1085:1090	COP-W1	1085:1090	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	7	38	contain	has	1092:1094	arg2	activity					1159:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1096:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL)	1096:1228	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	1	39	theme	new	130:132	arg1	polysaccharide					134:147	A new polysaccharide	128:147	A new polysaccharide named COP-W1	128:160	A new polysaccharide named COP-W1 was extracted from the root of Codonopsis tangshen Oliv using hot water extraction and ethanol precipitation.					
32172840	1	40	theme	hot	224:226	arg1	extraction					234:243	hot water extraction	224:243	hot water extraction	224:243	A new polysaccharide named COP-W1 was extracted from the root of Codonopsis tangshen Oliv using hot water extraction and ethanol precipitation.					
32172840	7	41	theme	1,1-diphenyl-2-picrylhydrazyl	1103:1131	arg1	activity					1159:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1096:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL)	1096:1228	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	0	42	from	Codonopsis	102:111	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	0	42	from	Codonopsis	102:111	arg1	polysaccharide					82:95	the COP-W1 polysaccharide	71:95	the COP-W1 polysaccharide from Codonopsis	71:111	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	0	42	from	Codonopsis	102:111	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	0	42	from	Codonopsis	102:111	arg1	activity					59:66	antioxidant activity	47:66	antioxidant activity	47:66	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	4	43	theme	magnetic	665:672	arg1	NMR					685:687	NMR	685:687	NMR	685:687	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	4	43	theme	magnetic	665:672	arg1	resonance					674:682	nuclear magnetic resonance	657:682	nuclear magnetic resonance (NMR) analysis	657:697	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	2	44	theme	Da	318:319	arg1	weight					297:302	a molecular weight	285:302	a molecular weight of 2.34 × 104 Da	285:319	COP-W1, with a molecular weight of 2.34 × 104 Da, was purified using gel permeation chromatography.					
32172840	7	45	theme	DPPH	1134:1137	arg1	activity					1159:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1096:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL)	1096:1228	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	7	46	from	mg/mL	1223:1227	arg1	IC50					1208:1211	IC50	1208:1211	IC50	1208:1211	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	7	46	from	mg/mL	1223:1227	arg1	concentration					1193:1205	half maximal inhibition concentration	1169:1205	half maximal inhibition concentration	1169:1205	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	2	47	theme	permeation	345:354	arg1	chromatography					356:369	gel permeation chromatography	341:369	gel permeation chromatography	341:369	COP-W1, with a molecular weight of 2.34 × 104 Da, was purified using gel permeation chromatography.					
32172840	4	48	theme	gas	608:610	arg1	GC-MS					645:649	GC-MS	645:649	GC-MS	645:649	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	4	48	theme	gas	608:610	arg1	spectrometry					631:642	gas chromatograph-mass spectrometry	608:642	gas chromatograph-mass spectrometry (GC-MS)	608:650	Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis.					
32172840	0	49	from	characterization	25:40	arg1	Codonopsis					102:111	Codonopsis	102:111	Codonopsis	102:111	Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv.					
32172840	7	50	theme	antioxidant	1048:1058	arg1	assays					1060:1065	antioxidant assays	1048:1065	antioxidant assays	1048:1065	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32172840	2	51	theme	gel	341:343	arg1	chromatography					356:369	gel permeation chromatography	341:369	gel permeation chromatography	341:369	COP-W1, with a molecular weight of 2.34 × 104 Da, was purified using gel permeation chromatography.					
32172840	7	52	theme	strong	1096:1101	arg1	activity					1159:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1096:1166	strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL)	1096:1228	In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC50, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.					
32852845	6	0	theme	Atomic	899:904	arg1	spectroscopy					917:928	Atomic absorption spectroscopy	899:928	Atomic absorption spectroscopy	899:928	Atomic absorption spectroscopy was used to quantify whole blood manganese levels.					
32852845	12	1	theme	precursor	1569:1577	arg1	A2G1S1					1588:1593	the asialo-agalactosylated precursor N-glycan A2G1S1	1542:1593	the asialo-agalactosylated precursor N-glycan A2G1S1	1542:1593	N-glycome using MALDI-TOF MS identified an increase of the asialo-agalactosylated precursor N-glycan A2G1S1 and a decrease in bisected structures.					
32852845	9	2	theme	c.608T>C	1289:1296	arg1	[p.F203S					1298:1305	the previously undescribed homozygous variant c.608T>C [p.F203S]	1243:1306	the previously undescribed homozygous variant c.608T>C [p.F203S] in SLC39A8	1243:1317	In patient 1, exome sequencing identified the previously undescribed homozygous variant c.608T>C [p.F203S] in SLC39A8.					
32852845	13	3	theme	glycosylation	1727:1739	arg1	changes					1741:1747	similar but less severe glycosylation changes	1703:1747	similar but less severe glycosylation changes	1703:1747	In addition, analysis of heterozygous CDG-allele carriers identified similar but less severe glycosylation changes.					
32852845	15	4	theme	glycosylation	1972:1984	arg1	spectrum					1947:1954	a spectrum	1945:1954	a spectrum of dysregulated glycosylation	1945:1984	These results emphasize that SLC39A8-CDG presents as a spectrum of dysregulated glycosylation, and MS is an important tool for identifying deficiencies not detected by conventional methods.					
32852845	1	5	theme	metabolic	172:180	arg1	disorders					182:190	inborn metabolic disorders	165:190	inborn metabolic disorders with multiorgan presentation	165:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	6	6	theme	manganese	963:971	arg1	levels					973:978	whole blood manganese levels	951:978	whole blood manganese levels	951:978	Atomic absorption spectroscopy was used to quantify whole blood manganese levels.					
32852845	8	7	theme	resonance	1089:1097	arg1	imaging					1099:1105	Magnetic resonance imaging	1080:1105	Magnetic resonance imaging (MRI)	1080:1111	Magnetic resonance imaging (MRI) revealed a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia.					
32852845	8	7	theme	resonance	1089:1097	arg1	MRI					1108:1110	MRI	1108:1110	MRI	1108:1110	Magnetic resonance imaging (MRI) revealed a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia.					
32852845	11	8	theme	blood	1425:1429	arg1	manganese					1431:1439	whole blood manganese	1419:1439	whole blood manganese	1419:1439	Both individuals showed a reduction of whole blood manganese, though transferrin glycosylation was normal.					
32852845	13	9	theme	similar	1703:1709	arg1	changes					1741:1747	similar but less severe glycosylation changes	1703:1747	similar but less severe glycosylation changes	1703:1747	In addition, analysis of heterozygous CDG-allele carriers identified similar but less severe glycosylation changes.					
32852845	5	10	theme	Transferrin	637:647	arg1	glycosylation					649:661	Transferrin glycosylation	637:661	Transferrin glycosylation	637:661	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	9	11	theme	homozygous	1270:1279	arg1	[p.F203S					1298:1305	the previously undescribed homozygous variant c.608T>C [p.F203S]	1243:1306	the previously undescribed homozygous variant c.608T>C [p.F203S] in SLC39A8	1243:1317	In patient 1, exome sequencing identified the previously undescribed homozygous variant c.608T>C [p.F203S] in SLC39A8.					
32852845	12	12	from	decrease	1601:1608	arg1	structures					1622:1631	bisected structures	1613:1631	bisected structures	1613:1631	N-glycome using MALDI-TOF MS identified an increase of the asialo-agalactosylated precursor N-glycan A2G1S1 and a decrease in bisected structures.					
32852845	8	13	with	syndrome	1135:1142	arg1	hyperintensities					1162:1177	bilateral T2 hyperintensities	1149:1177	bilateral T2 hyperintensities of the basal ganglia	1149:1198	Magnetic resonance imaging (MRI) revealed a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia.					
32852845	11	14	theme	transferrin	1449:1459	arg1	glycosylation					1461:1473	transferrin glycosylation	1449:1473	transferrin glycosylation	1449:1473	Both individuals showed a reduction of whole blood manganese, though transferrin glycosylation was normal.					
32852845	5	15	theme	matrix-assisted	804:818	arg1	spectrometry					880:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry	804:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	804:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	15	theme	matrix-assisted	804:818	arg1	MS					894:895	MS	894:895	MS	894:895	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	2	16	theme	severe	239:244	arg1	subtype					246:252	a severe subtype	237:252	a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8	237:319	SLC39A8-CDG is a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8, reducing levels of this essential cofactor for many enzymes including glycosyltransferases.					
32852845	2	16	theme	severe	239:244	arg1	SLC39A8-CDG					222:232	SLC39A8-CDG	222:232	SLC39A8-CDG	222:232	SLC39A8-CDG is a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8, reducing levels of this essential cofactor for many enzymes including glycosyltransferases.					
32852845	15	17	theme	conventional	2060:2071	arg1	methods					2073:2079	conventional methods	2060:2079	conventional methods	2060:2079	These results emphasize that SLC39A8-CDG presents as a spectrum of dysregulated glycosylation, and MS is an important tool for identifying deficiencies not detected by conventional methods.					
32852845	14	18	theme	transferrin	1812:1822	arg1	glycosylation					1824:1836	transferrin glycosylation	1812:1836	transferrin glycosylation	1812:1836	Despite its reliance as a clinical gold standard, analysis of transferrin glycosylation cannot be categorically used to rule out SLC39A8-CDG.					
32852845	13	19	theme	carriers	1683:1690	arg1	analysis					1647:1654	analysis	1647:1654	analysis of heterozygous CDG-allele carriers	1647:1690	In addition, analysis of heterozygous CDG-allele carriers identified similar but less severe glycosylation changes.					
32852845	5	20	theme	comprehensive	765:777	arg1	analysis					789:796	comprehensive N-glycome analysis	765:796	comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	765:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	2	21	theme	biallelic	264:272	arg1	mutations					274:282	biallelic mutations	264:282	biallelic mutations in the manganese transporter SLC39A8	264:319	SLC39A8-CDG is a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8, reducing levels of this essential cofactor for many enzymes including glycosyltransferases.					
32852845	5	22	theme	flight	856:861	arg1	spectrometry					880:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry	804:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	804:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	22	theme	flight	856:861	arg1	MS					894:895	MS	894:895	MS	894:895	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	7	23	theme	neurological	1056:1067	arg1	phenotype					1069:1077	a complex neurological phenotype	1046:1077	a complex neurological phenotype	1046:1077	Both patients presented with a severe, multisystem disorder, and a complex neurological phenotype.					
32852845	1	24	theme	growing	148:154	arg1	disorders					182:190	inborn metabolic disorders	165:190	inborn metabolic disorders with multiorgan presentation	165:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	1	24	theme	growing	148:154	arg1	disorders					109:117	Congenital disorders	98:117	Congenital disorders of glycosylation (CDG)	98:140	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	1	24	theme	growing	148:154	arg1	group					156:160	a growing group	146:160	a growing group of inborn metabolic disorders with multiorgan presentation	146:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	5	25	theme	MALDI-TOF	864:872	arg1	spectrometry					880:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry	804:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	804:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	25	theme	MALDI-TOF	864:872	arg1	MS					894:895	MS	894:895	MS	894:895	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	12	26	theme	bisected	1613:1620	arg1	structures					1622:1631	bisected structures	1613:1631	bisected structures	1613:1631	N-glycome using MALDI-TOF MS identified an increase of the asialo-agalactosylated precursor N-glycan A2G1S1 and a decrease in bisected structures.					
32852845	7	27	dep	disorder	1032:1039	arg1	a					1010:1010	a	1010:1010	a	1010:1010	Both patients presented with a severe, multisystem disorder, and a complex neurological phenotype.					
32852845	5	28	theme	desorption	826:835	arg1	spectrometry					880:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry	804:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	804:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	28	theme	desorption	826:835	arg1	MS					894:895	MS	894:895	MS	894:895	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	2	29	theme	manganese	291:299	arg1	SLC39A8					313:319	the manganese transporter SLC39A8	287:319	the manganese transporter SLC39A8	287:319	SLC39A8-CDG is a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8, reducing levels of this essential cofactor for many enzymes including glycosyltransferases.					
32852845	5	30	theme	time	848:851	arg1	spectrometry					880:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry	804:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	804:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	30	theme	time	848:851	arg1	MS					894:895	MS	894:895	MS	894:895	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	7	31	theme	multisystem	1020:1030	arg1	disorder					1032:1039	multisystem disorder	1020:1039	multisystem disorder	1020:1039	Both patients presented with a severe, multisystem disorder, and a complex neurological phenotype.					
32852845	0	32	theme	SLC39A8	78:84	arg1	deficiency					86:95	SLC39A8 deficiency	78:95	SLC39A8 deficiency	78:95	N-glycome analysis detects dysglycosylation missed by conventional methods in SLC39A8 deficiency.					
32852845	0	33	theme	N-glycome	0:8	arg1	analysis					10:17	N-glycome analysis	0:17	N-glycome analysis	0:17	N-glycome analysis detects dysglycosylation missed by conventional methods in SLC39A8 deficiency.					
32852845	8	34	theme	bilateral	1149:1157	arg1	hyperintensities					1162:1177	bilateral T2 hyperintensities	1149:1177	bilateral T2 hyperintensities of the basal ganglia	1149:1198	Magnetic resonance imaging (MRI) revealed a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia.					
32852845	7	35	theme	severe	1012:1017	arg1	disorder					1032:1039	multisystem disorder	1020:1039	multisystem disorder	1020:1039	Both patients presented with a severe, multisystem disorder, and a complex neurological phenotype.					
32852845	4	36	theme	neurodevelopmental	588:605	arg1	phenotypes					607:616	severe neurodevelopmental phenotypes	581:616	severe neurodevelopmental phenotypes suggestive of CDG	581:634	Exome and Sanger sequencing were performed in two patients with severe neurodevelopmental phenotypes suggestive of CDG.					
32852845	3	37	theme	serum	498:502	arg1	transferrin					504:514	serum transferrin	498:514	serum transferrin	498:514	The current diagnostic standard for disorders of N-glycosylation is the analysis of serum transferrin.					
32852845	6	38	used	used	934:937	arg2	spectroscopy					917:928	Atomic absorption spectroscopy	899:928	Atomic absorption spectroscopy	899:928	Atomic absorption spectroscopy was used to quantify whole blood manganese levels.					
32852845	6	39	theme	blood	957:961	arg1	levels					973:978	whole blood manganese levels	951:978	whole blood manganese levels	951:978	Atomic absorption spectroscopy was used to quantify whole blood manganese levels.					
32852845	1	40	theme	glycosylation	122:134	arg1	disorders					182:190	inborn metabolic disorders	165:190	inborn metabolic disorders with multiorgan presentation	165:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	1	40	theme	glycosylation	122:134	arg1	disorders					109:117	Congenital disorders	98:117	Congenital disorders of glycosylation (CDG)	98:140	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	1	40	theme	glycosylation	122:134	arg1	group					156:160	a growing group	146:160	a growing group of inborn metabolic disorders with multiorgan presentation	146:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	2	41	from	mutations	274:282	arg1	SLC39A8					313:319	the manganese transporter SLC39A8	287:319	the manganese transporter SLC39A8	287:319	SLC39A8-CDG is a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8, reducing levels of this essential cofactor for many enzymes including glycosyltransferases.					
32852845	4	42	theme	suggestive	618:627	arg1	phenotypes					607:616	severe neurodevelopmental phenotypes	581:616	severe neurodevelopmental phenotypes suggestive of CDG	581:634	Exome and Sanger sequencing were performed in two patients with severe neurodevelopmental phenotypes suggestive of CDG.					
32852845	5	43	theme	mass	875:878	arg1	spectrometry					880:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry	804:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	804:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	43	theme	mass	875:878	arg1	MS					894:895	MS	894:895	MS	894:895	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	2	44	theme	cofactor	356:363	arg1	levels					331:336	levels	331:336	levels of this essential cofactor for many enzymes including glycosyltransferases	331:411	SLC39A8-CDG is a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8, reducing levels of this essential cofactor for many enzymes including glycosyltransferases.					
32852845	0	45	theme	conventional	54:65	arg1	methods					67:73	conventional methods	54:73	conventional methods in SLC39A8 deficiency	54:95	N-glycome analysis detects dysglycosylation missed by conventional methods in SLC39A8 deficiency.					
32852845	8	46	theme	ganglia	1192:1198	arg1	hyperintensities					1162:1177	bilateral T2 hyperintensities	1149:1177	bilateral T2 hyperintensities of the basal ganglia	1149:1198	Magnetic resonance imaging (MRI) revealed a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia.					
32852845	0	47	dep	detects	19:25	arg1	missed					44:49	missed	44:49	detects dysglycosylation missed by conventional methods in SLC39A8 deficiency	19:95	N-glycome analysis detects dysglycosylation missed by conventional methods in SLC39A8 deficiency.					
32852845	14	48	used	used	1862:1865	arg2	analysis					1800:1807	analysis	1800:1807	analysis of transferrin glycosylation	1800:1836	Despite its reliance as a clinical gold standard, analysis of transferrin glycosylation cannot be categorically used to rule out SLC39A8-CDG.					
32852845	12	49	theme	asialo-agalactosylated	1546:1567	arg1	A2G1S1					1588:1593	the asialo-agalactosylated precursor N-glycan A2G1S1	1542:1593	the asialo-agalactosylated precursor N-glycan A2G1S1	1542:1593	N-glycome using MALDI-TOF MS identified an increase of the asialo-agalactosylated precursor N-glycan A2G1S1 and a decrease in bisected structures.					
32852845	9	50	theme	undescribed	1258:1268	arg1	[p.F203S					1298:1305	the previously undescribed homozygous variant c.608T>C [p.F203S]	1243:1306	the previously undescribed homozygous variant c.608T>C [p.F203S] in SLC39A8	1243:1317	In patient 1, exome sequencing identified the previously undescribed homozygous variant c.608T>C [p.F203S] in SLC39A8.					
32852845	2	51	theme	many	369:372	arg1	glycosyltransferases					392:411	glycosyltransferases	392:411	glycosyltransferases	392:411	SLC39A8-CDG is a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8, reducing levels of this essential cofactor for many enzymes including glycosyltransferases.					
32852845	2	51	theme	many	369:372	arg1	enzymes					374:380	many enzymes	369:380	many enzymes including glycosyltransferases	369:411	SLC39A8-CDG is a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8, reducing levels of this essential cofactor for many enzymes including glycosyltransferases.					
32852845	12	52	theme	N-glycan	1579:1586	arg1	A2G1S1					1588:1593	the asialo-agalactosylated precursor N-glycan A2G1S1	1542:1593	the asialo-agalactosylated precursor N-glycan A2G1S1	1542:1593	N-glycome using MALDI-TOF MS identified an increase of the asialo-agalactosylated precursor N-glycan A2G1S1 and a decrease in bisected structures.					
32852845	4	53	theme	CDG	632:634	arg1	suggestive					618:627	suggestive	618:627	suggestive	618:627	Exome and Sanger sequencing were performed in two patients with severe neurodevelopmental phenotypes suggestive of CDG.					
32852845	9	54	theme	variant	1281:1287	arg1	[p.F203S					1298:1305	the previously undescribed homozygous variant c.608T>C [p.F203S]	1243:1306	the previously undescribed homozygous variant c.608T>C [p.F203S] in SLC39A8	1243:1317	In patient 1, exome sequencing identified the previously undescribed homozygous variant c.608T>C [p.F203S] in SLC39A8.					
32852845	2	55	theme	transporter	301:311	arg1	SLC39A8					313:319	the manganese transporter SLC39A8	287:319	the manganese transporter SLC39A8	287:319	SLC39A8-CDG is a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8, reducing levels of this essential cofactor for many enzymes including glycosyltransferases.					
32852845	12	56	from	increase	1530:1537	arg1	structures					1622:1631	bisected structures	1613:1631	bisected structures	1613:1631	N-glycome using MALDI-TOF MS identified an increase of the asialo-agalactosylated precursor N-glycan A2G1S1 and a decrease in bisected structures.					
32852845	8	57	theme	Magnetic	1080:1087	arg1	imaging					1099:1105	Magnetic resonance imaging	1080:1105	Magnetic resonance imaging (MRI)	1080:1111	Magnetic resonance imaging (MRI) revealed a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia.					
32852845	8	57	theme	Magnetic	1080:1087	arg1	MRI					1108:1110	MRI	1108:1110	MRI	1108:1110	Magnetic resonance imaging (MRI) revealed a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia.					
32852845	15	58	theme	dysregulated	1959:1970	arg1	glycosylation					1972:1984	dysregulated glycosylation	1959:1984	dysregulated glycosylation	1959:1984	These results emphasize that SLC39A8-CDG presents as a spectrum of dysregulated glycosylation, and MS is an important tool for identifying deficiencies not detected by conventional methods.					
32852845	5	59	theme	liquid	696:701	arg1	HPLC					719:722	HPLC	719:722	HPLC	719:722	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	59	theme	liquid	696:701	arg1	chromatography					703:716	high-performance liquid chromatography	679:716	high-performance liquid chromatography (HPLC)	679:723	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	1	60	theme	inborn	165:170	arg1	disorders					182:190	inborn metabolic disorders	165:190	inborn metabolic disorders with multiorgan presentation	165:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	12	61	theme	MALDI-TOF	1503:1511	arg1	MS					1513:1514	MALDI-TOF MS	1503:1514	MALDI-TOF MS	1503:1514	N-glycome using MALDI-TOF MS identified an increase of the asialo-agalactosylated precursor N-glycan A2G1S1 and a decrease in bisected structures.					
32852845	11	62	theme	whole	1419:1423	arg1	manganese					1431:1439	whole blood manganese	1419:1439	whole blood manganese	1419:1439	Both individuals showed a reduction of whole blood manganese, though transferrin glycosylation was normal.					
32852845	1	63	theme	disorders	182:190	arg1	disorders					182:190	inborn metabolic disorders	165:190	inborn metabolic disorders with multiorgan presentation	165:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	1	63	theme	disorders	182:190	arg1	disorders					109:117	Congenital disorders	98:117	Congenital disorders of glycosylation (CDG)	98:140	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	1	63	theme	disorders	182:190	arg1	group					156:160	a growing group	146:160	a growing group of inborn metabolic disorders with multiorgan presentation	146:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	5	64	theme	N-glycome	779:787	arg1	analysis					789:796	comprehensive N-glycome analysis	765:796	comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	765:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	4	65	with	patients	567:574	arg1	phenotypes					607:616	severe neurodevelopmental phenotypes	581:616	severe neurodevelopmental phenotypes suggestive of CDG	581:634	Exome and Sanger sequencing were performed in two patients with severe neurodevelopmental phenotypes suggestive of CDG.					
32852845	1	66	theme	multiorgan	197:206	arg1	presentation					208:219	multiorgan presentation	197:219	multiorgan presentation	197:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	11	67	theme	manganese	1431:1439	arg1	reduction					1406:1414	a reduction	1404:1414	a reduction of whole blood manganese	1404:1439	Both individuals showed a reduction of whole blood manganese, though transferrin glycosylation was normal.					
32852845	14	68	theme	glycosylation	1824:1836	arg1	analysis					1800:1807	analysis	1800:1807	analysis of transferrin glycosylation	1800:1836	Despite its reliance as a clinical gold standard, analysis of transferrin glycosylation cannot be categorically used to rule out SLC39A8-CDG.					
32852845	13	69	theme	CDG-allele	1672:1681	arg1	carriers					1683:1690	heterozygous CDG-allele carriers	1659:1690	heterozygous CDG-allele carriers	1659:1690	In addition, analysis of heterozygous CDG-allele carriers identified similar but less severe glycosylation changes.					
32852845	5	70	theme	of	853:854	arg1	spectrometry					880:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry	804:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	804:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	70	theme	of	853:854	arg1	MS					894:895	MS	894:895	MS	894:895	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	13	71	theme	severe	1720:1725	arg1	changes					1741:1747	similar but less severe glycosylation changes	1703:1747	similar but less severe glycosylation changes	1703:1747	In addition, analysis of heterozygous CDG-allele carriers identified similar but less severe glycosylation changes.					
32852845	9	72	from	[p.F203S	1298:1305	arg1	SLC39A8					1311:1317	SLC39A8	1311:1317	SLC39A8	1311:1317	In patient 1, exome sequencing identified the previously undescribed homozygous variant c.608T>C [p.F203S] in SLC39A8.					
32852845	5	73	theme	laser	820:824	arg1	spectrometry					880:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry	804:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	804:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	73	theme	laser	820:824	arg1	MS					894:895	MS	894:895	MS	894:895	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	8	74	theme	Leigh-like	1124:1133	arg1	syndrome					1135:1142	a Leigh-like syndrome	1122:1142	a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia	1122:1198	Magnetic resonance imaging (MRI) revealed a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia.					
32852845	7	75	theme	complex	1048:1054	arg1	phenotype					1069:1077	a complex neurological phenotype	1046:1077	a complex neurological phenotype	1046:1077	Both patients presented with a severe, multisystem disorder, and a complex neurological phenotype.					
32852845	0	76	from	methods	67:73	arg1	deficiency					86:95	SLC39A8 deficiency	78:95	SLC39A8 deficiency	78:95	N-glycome analysis detects dysglycosylation missed by conventional methods in SLC39A8 deficiency.					
32852845	5	77	theme	ionization	837:846	arg1	spectrometry					880:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry	804:891	matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS)	804:896	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	77	theme	ionization	837:846	arg1	MS					894:895	MS	894:895	MS	894:895	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	78	theme	high-performance	679:694	arg1	HPLC					719:722	HPLC	719:722	HPLC	719:722	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	5	78	theme	high-performance	679:694	arg1	chromatography					703:716	high-performance liquid chromatography	679:716	high-performance liquid chromatography (HPLC)	679:723	Transferrin glycosylation was analyzed by high-performance liquid chromatography (HPLC) and isoelectric focusing in addition to comprehensive N-glycome analysis using matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS).					
32852845	4	79	theme	Sanger	527:532	arg1	sequencing					534:543	Sanger sequencing	527:543	Sanger sequencing	527:543	Exome and Sanger sequencing were performed in two patients with severe neurodevelopmental phenotypes suggestive of CDG.					
32852845	8	80	theme	T2	1159:1160	arg1	hyperintensities					1162:1177	bilateral T2 hyperintensities	1149:1177	bilateral T2 hyperintensities of the basal ganglia	1149:1198	Magnetic resonance imaging (MRI) revealed a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia.					
32852845	1	81	theme	Congenital	98:107	arg1	disorders					182:190	inborn metabolic disorders	165:190	inborn metabolic disorders with multiorgan presentation	165:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	1	81	theme	Congenital	98:107	arg1	disorders					109:117	Congenital disorders	98:117	Congenital disorders of glycosylation (CDG)	98:140	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	1	81	theme	Congenital	98:107	arg1	group					156:160	a growing group	146:160	a growing group of inborn metabolic disorders with multiorgan presentation	146:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	3	82	theme	N-glycosylation	463:477	arg1	disorders					450:458	disorders	450:458	disorders of N-glycosylation	450:477	The current diagnostic standard for disorders of N-glycosylation is the analysis of serum transferrin.					
32852845	3	83	theme	transferrin	504:514	arg1	standard					437:444	standard	437:444	standard	437:444	The current diagnostic standard for disorders of N-glycosylation is the analysis of serum transferrin.					
32852845	3	83	theme	transferrin	504:514	arg1	analysis					486:493	the analysis	482:493	the analysis of serum transferrin	482:514	The current diagnostic standard for disorders of N-glycosylation is the analysis of serum transferrin.					
32852845	8	84	theme	basal	1186:1190	arg1	ganglia					1192:1198	the basal ganglia	1182:1198	the basal ganglia	1182:1198	Magnetic resonance imaging (MRI) revealed a Leigh-like syndrome with bilateral T2 hyperintensities of the basal ganglia.					
32852845	6	85	theme	whole	951:955	arg1	levels					973:978	whole blood manganese levels	951:978	whole blood manganese levels	951:978	Atomic absorption spectroscopy was used to quantify whole blood manganese levels.					
32852845	1	86	with	disorders	182:190	arg1	presentation					208:219	multiorgan presentation	197:219	multiorgan presentation	197:219	Congenital disorders of glycosylation (CDG) are a growing group of inborn metabolic disorders with multiorgan presentation.					
32852845	4	87	theme	severe	581:586	arg1	phenotypes					607:616	severe neurodevelopmental phenotypes	581:616	severe neurodevelopmental phenotypes suggestive of CDG	581:634	Exome and Sanger sequencing were performed in two patients with severe neurodevelopmental phenotypes suggestive of CDG.					
32852845	9	88	theme	exome	1215:1219	arg1	sequencing					1221:1230	exome sequencing	1215:1230	exome sequencing	1215:1230	In patient 1, exome sequencing identified the previously undescribed homozygous variant c.608T>C [p.F203S] in SLC39A8.					
32852845	2	89	theme	essential	346:354	arg1	cofactor					356:363	this essential cofactor	341:363	this essential cofactor for many enzymes including glycosyltransferases	341:411	SLC39A8-CDG is a severe subtype caused by biallelic mutations in the manganese transporter SLC39A8, reducing levels of this essential cofactor for many enzymes including glycosyltransferases.					
32852845	12	90	theme	A2G1S1	1588:1593	arg1	increase					1530:1537	an increase	1527:1537	an increase of the asialo-agalactosylated precursor N-glycan A2G1S1	1527:1593	N-glycome using MALDI-TOF MS identified an increase of the asialo-agalactosylated precursor N-glycan A2G1S1 and a decrease in bisected structures.					
32852845	12	90	theme	A2G1S1	1588:1593	arg1	decrease					1601:1608	a decrease	1599:1608	a decrease in bisected structures	1599:1631	N-glycome using MALDI-TOF MS identified an increase of the asialo-agalactosylated precursor N-glycan A2G1S1 and a decrease in bisected structures.					
32852845	13	91	theme	heterozygous	1659:1670	arg1	carriers					1683:1690	heterozygous CDG-allele carriers	1659:1690	heterozygous CDG-allele carriers	1659:1690	In addition, analysis of heterozygous CDG-allele carriers identified similar but less severe glycosylation changes.					
32852845	6	92	theme	absorption	906:915	arg1	spectroscopy					917:928	Atomic absorption spectroscopy	899:928	Atomic absorption spectroscopy	899:928	Atomic absorption spectroscopy was used to quantify whole blood manganese levels.					
32852845	15	93	theme	important	2000:2008	arg1	tool					2010:2013	an important tool	1997:2013	an important tool for identifying deficiencies not detected by conventional methods	1997:2079	These results emphasize that SLC39A8-CDG presents as a spectrum of dysregulated glycosylation, and MS is an important tool for identifying deficiencies not detected by conventional methods.					
32852845	15	93	theme	important	2000:2008	arg1	MS					1991:1992	MS	1991:1992	MS	1991:1992	These results emphasize that SLC39A8-CDG presents as a spectrum of dysregulated glycosylation, and MS is an important tool for identifying deficiencies not detected by conventional methods.					
34822830	6	0	theme	chelating	1207:1215	arg1	ability					1217:1223	Fe2+ chelating ability	1202:1223	Fe2+ chelating ability	1202:1223	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	5	1	dep	content	987:993	arg1	the					961:963	the	961:963	the	961:963	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	0	2	theme	polysaccharides	91:105	arg1	effects					80:86	α-glucosidase inhibitory effects	55:86	α-glucosidase inhibitory effects	55:86	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	0	2	theme	polysaccharides	91:105	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	0	2	theme	polysaccharides	91:105	arg1	activities					40:49	antioxidant activities	28:49	antioxidant activities	28:49	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	0	3	from	properties	16:25	arg1	fructus					120:126	Evodiae fructus	112:126	Evodiae fructus	112:126	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	6	4	theme	Fe2+	1202:1205	arg1	ability					1217:1223	Fe2+ chelating ability	1202:1223	Fe2+ chelating ability	1202:1223	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	8	5	theme	alkaline	1459:1466	arg1	solvents					1468:1475	alkaline solvents	1459:1475	alkaline solvents	1459:1475	Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.					
34822830	6	6	theme	antioxidant	1113:1123	arg1	DPPH					1153:1156	DPPH	1153:1156	DPPH	1153:1156	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	6	theme	antioxidant	1113:1123	arg1	activities					1125:1134	relatively stronger antioxidant activities	1093:1134	relatively stronger antioxidant activities	1093:1134	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	6	theme	antioxidant	1113:1123	arg1	ABTS					1147:1150	ABTS	1147:1150	ABTS	1147:1150	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	6	theme	antioxidant	1113:1123	arg1	ability					1217:1223	Fe2+ chelating ability	1202:1223	Fe2+ chelating ability	1202:1223	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	6	theme	antioxidant	1113:1123	arg1	effects					1254:1260	α-glucosidase inhibitory effects	1229:1260	α-glucosidase inhibitory effects	1229:1260	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	1	7	theme	water + 0.5 M	261:273	arg1	HCl					244:246	0.5 M HCl	238:246	0.5 M HCl	238:246	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	7	theme	water + 0.5 M	261:273	arg1	ERP-AK					338:343	ERP-AK	338:343	ERP-AK	338:343	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	7	theme	water + 0.5 M	261:273	arg1	ERP-W-AC					346:353	ERP-W-AC	346:353	ERP-W-AC	346:353	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	7	theme	water + 0.5 M	261:273	arg1	NaOH					297:300	water + 0.5 M NaOH	283:300	water + 0.5 M NaOH	283:300	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	7	theme	water + 0.5 M	261:273	arg1	ERP-W					323:327	ERP-W	323:327	ERP-W	323:327	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	7	theme	water + 0.5 M	261:273	arg1	water					231:235	water	231:235	water	231:235	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	7	theme	water + 0.5 M	261:273	arg1	NaOH					255:258	0.5 M NaOH	249:258	0.5 M NaOH	249:258	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	7	theme	water + 0.5 M	261:273	arg1	ERP-AC					330:335	ERP-AC	330:335	ERP-AC	330:335	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	7	theme	water + 0.5 M	261:273	arg1	ERP-W-AK					359:366	ERP-W-AK	359:366	ERP-W-AK	359:366	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	7	theme	water + 0.5 M	261:273	arg1	HCl					275:277	water + 0.5 M HCl	261:277	water + 0.5 M HCl	261:277	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	0	8	theme	Evodiae	112:118	arg1	fructus					120:126	Evodiae fructus	112:126	Evodiae fructus	112:126	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	5	9	theme	molecular	1007:1015	arg1	weight					1017:1022	molecular weight	1007:1022	molecular weight	1007:1022	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	6	10	theme	stronger	1104:1111	arg1	DPPH					1153:1156	DPPH	1153:1156	DPPH	1153:1156	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	10	theme	stronger	1104:1111	arg1	activities					1125:1134	relatively stronger antioxidant activities	1093:1134	relatively stronger antioxidant activities	1093:1134	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	10	theme	stronger	1104:1111	arg1	ABTS					1147:1150	ABTS	1147:1150	ABTS	1147:1150	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	10	theme	stronger	1104:1111	arg1	ability					1217:1223	Fe2+ chelating ability	1202:1223	Fe2+ chelating ability	1202:1223	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	10	theme	stronger	1104:1111	arg1	effects					1254:1260	α-glucosidase inhibitory effects	1229:1260	α-glucosidase inhibitory effects	1229:1260	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	11	theme	scavenging	1179:1188	arg1	activities					1190:1199	scavenging activities	1179:1199	scavenging activities	1179:1199	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	0	12	theme	α-glucosidase	55:67	arg1	effects					80:86	α-glucosidase inhibitory effects	55:86	α-glucosidase inhibitory effects	55:86	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	7	13	dep	properties	1338:1347	arg1	properties					1338:1347	their physicochemical properties	1316:1347	their physicochemical properties	1316:1347	Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight.					
34822830	7	13	dep	properties	1338:1347	arg1	composition					1375:1385	monosaccharide composition	1360:1385	monosaccharide composition	1360:1385	Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight.					
34822830	7	13	dep	properties	1338:1347	arg1	weight					1401:1406	molecular weight	1391:1406	molecular weight	1391:1406	Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight.					
34822830	3	14	theme	acidic	557:562	arg1	heteropolysaccharides					564:584	acidic heteropolysaccharides	557:584	acidic heteropolysaccharides	557:584	Physico-chemical analysis showed that they were acidic heteropolysaccharides, which had α- and β-configurations.					
34822830	8	15	theme	promising	1520:1528	arg1	antioxidants					1538:1549	promising natural antioxidants	1520:1549	promising natural antioxidants	1520:1549	Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.					
34822830	8	15	theme	promising	1520:1528	arg1	polysaccharides					1420:1434	polysaccharides	1420:1434	polysaccharides extracted by water and alkaline solvents from Evodiae fructus	1420:1496	Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.					
34822830	2	16	theme	α-glucosidase	444:456	arg1	effects					469:475	α-glucosidase inhibitory effects	444:475	α-glucosidase inhibitory effects	444:475	Their physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects were investigated and compared.					
34822830	0	17	from	activities	40:49	arg1	fructus					120:126	Evodiae fructus	112:126	Evodiae fructus	112:126	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	6	18	theme	O2-	1166:1168	arg1	radicals					1170:1177	O2- radicals	1166:1177	O2- radicals	1166:1177	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	8	19	theme	natural	1530:1536	arg1	antioxidants					1538:1549	promising natural antioxidants	1520:1549	promising natural antioxidants	1520:1549	Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.					
34822830	8	19	theme	natural	1530:1536	arg1	polysaccharides					1420:1434	polysaccharides	1420:1434	polysaccharides extracted by water and alkaline solvents from Evodiae fructus	1420:1496	Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.					
34822830	7	20	theme	monosaccharide	1360:1373	arg1	properties					1338:1347	their physicochemical properties	1316:1347	their physicochemical properties	1316:1347	Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight.					
34822830	7	20	theme	monosaccharide	1360:1373	arg1	composition					1375:1385	monosaccharide composition	1360:1385	monosaccharide composition	1360:1385	Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight.					
34822830	8	21	theme	medicine	1596:1603	arg1	industries					1605:1614	the food and medicine industries	1583:1614	the food and medicine industries	1583:1614	Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.					
34822830	0	22	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	3	23	contain	had	593:595	arg1	heteropolysaccharides					564:584	acidic heteropolysaccharides	557:584	acidic heteropolysaccharides	557:584	Physico-chemical analysis showed that they were acidic heteropolysaccharides, which had α- and β-configurations.					
34822830	3	23	contain	had	593:595	arg2	β-configurations					604:619	β-configurations	604:619	β-configurations	604:619	Physico-chemical analysis showed that they were acidic heteropolysaccharides, which had α- and β-configurations.					
34822830	3	23	contain	had	593:595	arg2	α-					597:598	α-	597:598	α-	597:598	Physico-chemical analysis showed that they were acidic heteropolysaccharides, which had α- and β-configurations.					
34822830	5	24	theme	best	823:826	arg1	stability					836:844	the best thermal stability	819:844	the best thermal stability	819:844	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	0	25	theme	antioxidant	28:38	arg1	activities					40:49	antioxidant activities	28:49	antioxidant activities	28:49	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	1	26	theme	water + 0.5 M	283:295	arg1	HCl					244:246	0.5 M HCl	238:246	0.5 M HCl	238:246	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	26	theme	water + 0.5 M	283:295	arg1	ERP-AK					338:343	ERP-AK	338:343	ERP-AK	338:343	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	26	theme	water + 0.5 M	283:295	arg1	ERP-W-AC					346:353	ERP-W-AC	346:353	ERP-W-AC	346:353	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	26	theme	water + 0.5 M	283:295	arg1	NaOH					297:300	water + 0.5 M NaOH	283:300	water + 0.5 M NaOH	283:300	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	26	theme	water + 0.5 M	283:295	arg1	ERP-W					323:327	ERP-W	323:327	ERP-W	323:327	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	26	theme	water + 0.5 M	283:295	arg1	water					231:235	water	231:235	water	231:235	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	26	theme	water + 0.5 M	283:295	arg1	NaOH					255:258	0.5 M NaOH	249:258	0.5 M NaOH	249:258	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	26	theme	water + 0.5 M	283:295	arg1	ERP-AC					330:335	ERP-AC	330:335	ERP-AC	330:335	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	26	theme	water + 0.5 M	283:295	arg1	ERP-W-AK					359:366	ERP-W-AK	359:366	ERP-W-AK	359:366	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	26	theme	water + 0.5 M	283:295	arg1	HCl					275:277	water + 0.5 M HCl	261:277	water + 0.5 M HCl	261:277	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	5	27	theme	lower	897:901	arg1	weight					913:918	lower molecular weight	897:918	lower molecular weight (83.6 and 41.6 kDa)	897:938	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	6	28	dep	DPPH	1153:1156	arg1	activities					1190:1199	scavenging activities	1179:1199	scavenging activities	1179:1199	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	2	29	theme	antioxidant	417:427	arg1	activities					429:438	antioxidant activities	417:438	antioxidant activities	417:438	Their physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects were investigated and compared.					
34822830	0	30	theme	different	141:149	arg1	solvents					151:158	different solvents	141:158	different solvents	141:158	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	5	31	theme	molecular	903:911	arg1	weight					913:918	lower molecular weight	897:918	lower molecular weight (83.6 and 41.6 kDa)	897:938	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	6	32	theme	Biological	1025:1034	arg1	evaluation					1036:1045	Biological evaluation	1025:1045	Biological evaluation	1025:1045	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	2	33	theme	physicochemical	389:403	arg1	properties					405:414	Their physicochemical properties	383:414	Their physicochemical properties	383:414	Their physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects were investigated and compared.					
34822830	5	34	contain	had	785:787	arg2	yield					801:805	the highest yield	789:805	the highest yield (24.5%)	789:813	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	34	contain	had	785:787	arg2	%					812:812	24.5%	808:812	24.5%	808:812	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	34	contain	had	785:787	arg2	stability					836:844	the best thermal stability	819:844	the best thermal stability	819:844	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	34	contain	had	785:787	arg1	ERP-AK					778:783	ERP-AK	778:783	ERP-AK	778:783	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	7	35	theme	physicochemical	1322:1336	arg1	properties					1338:1347	their physicochemical properties	1316:1347	their physicochemical properties	1316:1347	Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight.					
34822830	7	35	theme	physicochemical	1322:1336	arg1	composition					1375:1385	monosaccharide composition	1360:1385	monosaccharide composition	1360:1385	Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight.					
34822830	7	35	theme	physicochemical	1322:1336	arg1	weight					1401:1406	molecular weight	1391:1406	molecular weight	1391:1406	Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight.					
34822830	0	36	theme	inhibitory	69:78	arg1	effects					80:86	α-glucosidase inhibitory effects	55:86	α-glucosidase inhibitory effects	55:86	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	8	37	theme	α-glucosidase	1555:1567	arg1	polysaccharides					1420:1434	polysaccharides	1420:1434	polysaccharides extracted by water and alkaline solvents from Evodiae fructus	1420:1496	Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.					
34822830	8	37	theme	α-glucosidase	1555:1567	arg1	inhibitors					1569:1578	α-glucosidase inhibitors	1555:1578	α-glucosidase inhibitors	1555:1578	Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.					
34822830	6	38	theme	inhibitory	1243:1252	arg1	effects					1254:1260	α-glucosidase inhibitory effects	1229:1260	α-glucosidase inhibitory effects	1229:1260	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	0	39	from	effects	80:86	arg1	fructus					120:126	Evodiae fructus	112:126	Evodiae fructus	112:126	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	2	40	theme	inhibitory	458:467	arg1	effects					469:475	α-glucosidase inhibitory effects	444:475	α-glucosidase inhibitory effects	444:475	Their physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects were investigated and compared.					
34822830	6	41	theme	α-glucosidase	1229:1241	arg1	effects					1254:1260	α-glucosidase inhibitory effects	1229:1260	α-glucosidase inhibitory effects	1229:1260	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	5	42	theme	neutral	973:979	arg1	content					987:993	neutral sugar content	973:993	neutral sugar content (50.7%)	973:1001	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	42	theme	neutral	973:979	arg1	%					1000:1000	50.7%	996:1000	50.7%	996:1000	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	43	theme	highest	965:971	arg1	content					987:993	neutral sugar content	973:993	neutral sugar content (50.7%)	973:1001	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	43	theme	highest	965:971	arg1	%					1000:1000	50.7%	996:1000	50.7%	996:1000	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	3	44	theme	Physico-chemical	509:524	arg1	analysis					526:533	Physico-chemical analysis	509:533	Physico-chemical analysis	509:533	Physico-chemical analysis showed that they were acidic heteropolysaccharides, which had α- and β-configurations.					
34822830	5	45	theme	sugar	981:985	arg1	content					987:993	neutral sugar content	973:993	neutral sugar content (50.7%)	973:1001	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	45	theme	sugar	981:985	arg1	%					1000:1000	50.7%	996:1000	50.7%	996:1000	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	46	dep	weight	913:918	arg1	41.6 kDa					930:937	41.6 kDa	930:937	41.6 kDa	930:937	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	46	dep	weight	913:918	arg1	83.6					921:924	83.6	921:924	83.6	921:924	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	1	47	theme	0.5 M	238:242	arg1	HCl					244:246	0.5 M HCl	238:246	0.5 M HCl	238:246	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	47	theme	0.5 M	238:242	arg1	ERP-AK					338:343	ERP-AK	338:343	ERP-AK	338:343	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	47	theme	0.5 M	238:242	arg1	ERP-W-AC					346:353	ERP-W-AC	346:353	ERP-W-AC	346:353	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	47	theme	0.5 M	238:242	arg1	NaOH					297:300	water + 0.5 M NaOH	283:300	water + 0.5 M NaOH	283:300	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	47	theme	0.5 M	238:242	arg1	ERP-W					323:327	ERP-W	323:327	ERP-W	323:327	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	47	theme	0.5 M	238:242	arg1	water					231:235	water	231:235	water	231:235	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	47	theme	0.5 M	238:242	arg1	NaOH					255:258	0.5 M NaOH	249:258	0.5 M NaOH	249:258	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	47	theme	0.5 M	238:242	arg1	ERP-AC					330:335	ERP-AC	330:335	ERP-AC	330:335	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	47	theme	0.5 M	238:242	arg1	ERP-W-AK					359:366	ERP-W-AK	359:366	ERP-W-AK	359:366	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	47	theme	0.5 M	238:242	arg1	HCl					275:277	water + 0.5 M HCl	261:277	water + 0.5 M HCl	261:277	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	7	48	theme	molecular	1391:1399	arg1	properties					1338:1347	their physicochemical properties	1316:1347	their physicochemical properties	1316:1347	Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight.					
34822830	7	48	theme	molecular	1391:1399	arg1	weight					1401:1406	molecular weight	1391:1406	molecular weight	1391:1406	Moreover, these actions were considerably related to their physicochemical properties especially monosaccharide composition and molecular weight.					
34822830	0	49	from	fructus	120:126	arg1	polysaccharides					91:105	polysaccharides	91:105	polysaccharides from Evodiae fructus	91:126	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	0	49	from	fructus	120:126	arg1	effects					80:86	α-glucosidase inhibitory effects	55:86	α-glucosidase inhibitory effects	55:86	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	0	49	from	fructus	120:126	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	0	49	from	fructus	120:126	arg1	activities					40:49	antioxidant activities	28:49	antioxidant activities	28:49	Physicochemical properties, antioxidant activities and α-glucosidase inhibitory effects of polysaccharides from Evodiae fructus extracted by different solvents.					
34822830	1	50	from	fructus	205:211	arg1	polysaccharides					176:190	polysaccharides	176:190	polysaccharides from Evodiae fructus	176:211	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	5	51	theme	better	874:879	arg1	homogeneity					881:891	better homogeneity	874:891	better homogeneity	874:891	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	52	contain	possessed	951:959	arg2	%					1000:1000	50.7%	996:1000	50.7%	996:1000	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	52	contain	possessed	951:959	arg2	weight					1017:1022	molecular weight	1007:1022	molecular weight	1007:1022	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	52	contain	possessed	951:959	arg1	ERP-W					945:949	ERP-W	945:949	ERP-W	945:949	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	52	contain	possessed	951:959	arg2	content					987:993	neutral sugar content	973:993	neutral sugar content (50.7%)	973:1001	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	8	53	theme	food	1587:1590	arg1	industries					1605:1614	the food and medicine industries	1583:1614	the food and medicine industries	1583:1614	Therefore, polysaccharides extracted by water and alkaline solvents from Evodiae fructus could be developed as promising natural antioxidants and α-glucosidase inhibitors in the food and medicine industries.					
34822830	5	54	theme	thermal	828:834	arg1	stability					836:844	the best thermal stability	819:844	the best thermal stability	819:844	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	55	theme	highest	793:799	arg1	yield					801:805	the highest yield	789:805	the highest yield (24.5%)	789:813	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	5	55	theme	highest	793:799	arg1	%					812:812	24.5%	808:812	24.5%	808:812	ERP-AK had the highest yield (24.5%) and the best thermal stability, ERP-AC and ERP-W-AC showed better homogeneity and lower molecular weight (83.6 and 41.6 kDa), and ERP-W possessed the highest neutral sugar content (50.7%) and molecular weight.					
34822830	6	56	contain	had	1089:1091	arg2	ability					1217:1223	Fe2+ chelating ability	1202:1223	Fe2+ chelating ability	1202:1223	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	56	contain	had	1089:1091	arg1	ERP-W					1062:1066	ERP-W	1062:1066	ERP-W	1062:1066	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	56	contain	had	1089:1091	arg2	effects					1254:1260	α-glucosidase inhibitory effects	1229:1260	α-glucosidase inhibitory effects	1229:1260	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	56	contain	had	1089:1091	arg2	activities					1125:1134	relatively stronger antioxidant activities	1093:1134	relatively stronger antioxidant activities	1093:1134	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	56	contain	had	1089:1091	arg2	ABTS					1147:1150	ABTS	1147:1150	ABTS	1147:1150	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	56	contain	had	1089:1091	arg1	ERP-AK					1069:1074	ERP-AK	1069:1074	ERP-AK	1069:1074	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	56	contain	had	1089:1091	arg2	DPPH					1153:1156	DPPH	1153:1156	DPPH	1153:1156	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	6	56	contain	had	1089:1091	arg1	ERP-W-AK					1080:1087	ERP-W-AK	1080:1087	ERP-W-AK	1080:1087	Biological evaluation indicated that ERP-W, ERP-AK and ERP-W-AK had relatively stronger antioxidant activities, including ABTS, DPPH, OH and O2- radicals scavenging activities, Fe2+ chelating ability and α-glucosidase inhibitory effects.					
34822830	1	57	theme	0.5 M	249:253	arg1	HCl					244:246	0.5 M HCl	238:246	0.5 M HCl	238:246	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	57	theme	0.5 M	249:253	arg1	ERP-AK					338:343	ERP-AK	338:343	ERP-AK	338:343	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	57	theme	0.5 M	249:253	arg1	ERP-W-AC					346:353	ERP-W-AC	346:353	ERP-W-AC	346:353	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	57	theme	0.5 M	249:253	arg1	NaOH					297:300	water + 0.5 M NaOH	283:300	water + 0.5 M NaOH	283:300	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	57	theme	0.5 M	249:253	arg1	ERP-W					323:327	ERP-W	323:327	ERP-W	323:327	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	57	theme	0.5 M	249:253	arg1	water					231:235	water	231:235	water	231:235	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	57	theme	0.5 M	249:253	arg1	NaOH					255:258	0.5 M NaOH	249:258	0.5 M NaOH	249:258	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	57	theme	0.5 M	249:253	arg1	ERP-AC					330:335	ERP-AC	330:335	ERP-AC	330:335	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	57	theme	0.5 M	249:253	arg1	ERP-W-AK					359:366	ERP-W-AK	359:366	ERP-W-AK	359:366	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34822830	1	57	theme	0.5 M	249:253	arg1	HCl					275:277	water + 0.5 M HCl	261:277	water + 0.5 M HCl	261:277	In this study, polysaccharides from Evodiae fructus were extracted by water, 0.5 M HCl, 0.5 M NaOH, water + 0.5 M HCl and water + 0.5 M NaOH, which were named as ERP-W, ERP-AC, ERP-AK, ERP-W-AC and ERP-W-AK, respectively.					
34664596	9	0	theme	dynamic	1502:1508	arg1	changes					1510:1516	the dynamic changes	1498:1516	the dynamic changes in the gut microbiota	1498:1538	Furthermore, 16S rDNA gene sequencing was used to monitor the dynamic changes in the gut microbiota.					
34664596	6	1	theme	hematoxylin	995:1005	arg1	staining					1017:1024	hematoxylin and eosin staining	995:1024	hematoxylin and eosin staining	995:1024	Moreover, hematoxylin and eosin staining showed that AH enhanced the number of intraepithelial lymphocytes (IELs) in the small intestine.					
34664596	9	2	theme	gut	1525:1527	arg1	microbiota					1529:1538	the gut microbiota	1521:1538	the gut microbiota	1521:1538	Furthermore, 16S rDNA gene sequencing was used to monitor the dynamic changes in the gut microbiota.					
34664596	7	3	from	ratio	1150:1154	arg1	propria					1208:1214	the lamina propria	1197:1214	the lamina propria	1197:1214	An obvious increase in the ratio of IgA+ cells of AH-treatment samples in the lamina propria was also detected by immunohistochemical staining.					
34664596	6	4	theme	lymphocytes	1080:1090	arg1	number					1054:1059	the number	1050:1059	the number of intraepithelial lymphocytes (IELs) in the small intestine	1050:1120	Moreover, hematoxylin and eosin staining showed that AH enhanced the number of intraepithelial lymphocytes (IELs) in the small intestine.					
34664596	12	5	theme	intestinal	1977:1986	arg1	immunity					1996:2003	murine intestinal mucosal immunity	1970:2003	murine intestinal mucosal immunity	1970:2003	In summary, these results indicated that AH could be used as a prebiotic to enhance murine intestinal mucosal immunity and to modulate the gut microbiome.					
34664596	1	6	contain	possess	249:255	arg1	AH					190:191	AH	190:191	AH	190:191	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	6	contain	possess	249:255	arg1	polysaccharides					173:187	Alhagi honey polysaccharides	160:187	Alhagi honey polysaccharides (AH)	160:192	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	6	contain	possess	249:255	arg1	supplements					331:341	dietary supplements	323:341	dietary supplements in traditional Chinese medicine	323:373	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	6	contain	possess	249:255	arg2	activities					283:292	excellent pharmacological activities	257:292	excellent pharmacological activities	257:292	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	6	contain	possess	249:255	arg1	component					209:217	a main active component	195:217	a main active component of Alhagi honey	195:233	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	10	7	dep	diversity	1698:1706	arg1	the					1694:1696	the	1694:1696	the	1694:1696	The result revealed that AH significantly increased the indexes of Shannon and obviously decreased the indexes of Simpson, suggesting the enhancement of the diversity and richness of the intestinal microbiome.					
34664596	3	8	theme	consecutive	674:684	arg1	days					686:689	7 consecutive days	672:689	7 consecutive days	672:689	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	8	9	from	ratio	1311:1315	arg1	nodes					1337:1341	mesenteric lymph nodes	1320:1341	mesenteric lymph nodes	1320:1341	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	2	10	theme	microbiome	529:538	arg1	function					493:500	murine intestinal mucosal immune function	460:500	murine intestinal mucosal immune function	460:500	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	2	10	theme	microbiome	529:538	arg1	composition					506:516	composition	506:516	composition	506:516	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	3	11	theme	intragastric	565:576	arg1	administration					578:591	daily intragastric administration	559:591	daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days	559:689	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	5	12	theme	control	968:974	arg1	samples					976:982	the control samples	964:982	the control samples	964:982	Simultaneously, AH also significantly stimulated IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, and TNF-α cytokine secretion as compared to the control samples.					
34664596	3	13	theme	ICR	541:543	arg1	mice					545:548	ICR mice	541:548	ICR mice	541:548	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	12	14	used	used	1939:1942	arg2	AH					1927:1928	AH	1927:1928	AH	1927:1928	In summary, these results indicated that AH could be used as a prebiotic to enhance murine intestinal mucosal immunity and to modulate the gut microbiome.					
34664596	12	14	used	used	1939:1942	arg2	prebiotic					1949:1957	a prebiotic	1947:1957	a prebiotic	1947:1957	In summary, these results indicated that AH could be used as a prebiotic to enhance murine intestinal mucosal immunity and to modulate the gut microbiome.					
34664596	0	15	theme	flora	145:149	arg1	structure					121:129	the structure	117:129	the structure of intestinal flora in mice	117:157	Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice.					
34664596	11	16	theme	probiotics	1825:1834	arg1	abundance					1812:1820	the abundance	1808:1820	the abundance of probiotics	1808:1834	Moreover, AH modulated the gut microbiome via increasing the abundance of probiotics and decreasing the levels of pathogenic bacteria.					
34664596	4	17	from	increases	768:776	arg1	sIgA					809:812	sIgA	809:812	sIgA	809:812	Results indicated an improvement in the intestinal barrier function through increases in secretory immunoglobulin A (sIgA) and β-defensins.					
34664596	4	17	from	increases	768:776	arg1	β-defensins					819:829	β-defensins	819:829	β-defensins	819:829	Results indicated an improvement in the intestinal barrier function through increases in secretory immunoglobulin A (sIgA) and β-defensins.					
34664596	4	17	from	increases	768:776	arg1	A					806:806	secretory immunoglobulin A	781:806	secretory immunoglobulin A (sIgA)	781:813	Results indicated an improvement in the intestinal barrier function through increases in secretory immunoglobulin A (sIgA) and β-defensins.					
34664596	1	18	theme	active	202:207	arg1	component					209:217	a main active component	195:217	a main active component of Alhagi honey	195:233	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	18	theme	active	202:207	arg1	polysaccharides					173:187	Alhagi honey polysaccharides	160:187	Alhagi honey polysaccharides (AH)	160:192	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	8	19	theme	lymph	1331:1335	arg1	nodes					1337:1341	mesenteric lymph nodes	1320:1341	mesenteric lymph nodes	1320:1341	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	10	20	theme	Simpson	1655:1661	arg1	indexes					1644:1650	the indexes	1640:1650	the indexes of Simpson	1640:1661	The result revealed that AH significantly increased the indexes of Shannon and obviously decreased the indexes of Simpson, suggesting the enhancement of the diversity and richness of the intestinal microbiome.					
34664596	1	21	used	used	315:318	arg2	polysaccharides					173:187	Alhagi honey polysaccharides	160:187	Alhagi honey polysaccharides (AH)	160:192	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	21	used	used	315:318	arg2	supplements					331:341	dietary supplements	323:341	dietary supplements in traditional Chinese medicine	323:373	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	21	used	used	315:318	arg2	component					209:217	a main active component	195:217	a main active component of Alhagi honey	195:233	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	21	used	used	315:318	arg2	AH					190:191	AH	190:191	AH	190:191	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	6	22	theme	small	1106:1110	arg1	intestine					1112:1120	the small intestine	1102:1120	the small intestine	1102:1120	Moreover, hematoxylin and eosin staining showed that AH enhanced the number of intraepithelial lymphocytes (IELs) in the small intestine.					
34664596	7	23	theme	immunohistochemical	1237:1255	arg1	staining					1257:1264	immunohistochemical staining	1237:1264	immunohistochemical staining	1237:1264	An obvious increase in the ratio of IgA+ cells of AH-treatment samples in the lamina propria was also detected by immunohistochemical staining.					
34664596	2	24	theme	mucosal	478:484	arg1	function					493:500	murine intestinal mucosal immune function	460:500	murine intestinal mucosal immune function	460:500	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	7	25	theme	obvious	1126:1132	arg1	increase					1134:1141	An obvious increase	1123:1141	An obvious increase in the ratio of IgA+ cells of AH-treatment samples in the lamina propria	1123:1214	An obvious increase in the ratio of IgA+ cells of AH-treatment samples in the lamina propria was also detected by immunohistochemical staining.					
34664596	2	26	theme	murine	460:465	arg1	function					493:500	murine intestinal mucosal immune function	460:500	murine intestinal mucosal immune function	460:500	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	1	27	theme	dietary	323:329	arg1	polysaccharides					173:187	Alhagi honey polysaccharides	160:187	Alhagi honey polysaccharides (AH)	160:192	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	27	theme	dietary	323:329	arg1	supplements					331:341	dietary supplements	323:341	dietary supplements in traditional Chinese medicine	323:373	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	28	theme	honey	167:171	arg1	AH					190:191	AH	190:191	AH	190:191	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	28	theme	honey	167:171	arg1	polysaccharides					173:187	Alhagi honey polysaccharides	160:187	Alhagi honey polysaccharides (AH)	160:192	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	28	theme	honey	167:171	arg1	supplements					331:341	dietary supplements	323:341	dietary supplements in traditional Chinese medicine	323:373	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	28	theme	honey	167:171	arg1	component					209:217	a main active component	195:217	a main active component of Alhagi honey	195:233	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	0	29	theme	immunity	97:104	arg1	improvement					67:77	the improvement	63:77	the improvement of the intestinal immunity regulating the structure of intestinal flora in mice	63:157	Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice.					
34664596	1	30	theme	Chinese	358:364	arg1	medicine					366:373	traditional Chinese medicine	346:373	traditional Chinese medicine	346:373	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	4	31	theme	secretory	781:789	arg1	sIgA					809:812	sIgA	809:812	sIgA	809:812	Results indicated an improvement in the intestinal barrier function through increases in secretory immunoglobulin A (sIgA) and β-defensins.					
34664596	4	31	theme	secretory	781:789	arg1	A					806:806	secretory immunoglobulin A	781:806	secretory immunoglobulin A (sIgA)	781:813	Results indicated an improvement in the intestinal barrier function through increases in secretory immunoglobulin A (sIgA) and β-defensins.					
34664596	0	32	theme	Alhagi	19:24	arg1	polysaccharides					32:46	Alhagi honey polysaccharides	19:46	Alhagi honey polysaccharides	19:46	Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice.					
34664596	1	33	theme	pharmacological	267:281	arg1	activities					283:292	excellent pharmacological activities	257:292	excellent pharmacological activities	257:292	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	9	34	theme	16S	1453:1455	arg1	sequencing					1467:1476	16S rDNA gene sequencing	1453:1476	16S rDNA gene sequencing	1453:1476	Furthermore, 16S rDNA gene sequencing was used to monitor the dynamic changes in the gut microbiota.					
34664596	2	35	theme	AH	454:455	arg1	effect					444:449	the heath effect	434:449	the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome	434:538	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	8	36	theme	control	1425:1431	arg1	group					1433:1437	the control group	1421:1437	the control group	1421:1437	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	8	37	theme	CD3+	1284:1287	arg1	higher					1401:1406	higher	1401:1406	higher	1401:1406	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	8	37	theme	CD3+	1284:1287	arg1	ratio					1311:1315	the CD3+, CD4+ and CD8+ T-cell ratio	1280:1315	the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment	1280:1381	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	6	38	from	number	1054:1059	arg1	intestine					1112:1120	the small intestine	1102:1120	the small intestine	1102:1120	Moreover, hematoxylin and eosin staining showed that AH enhanced the number of intraepithelial lymphocytes (IELs) in the small intestine.					
34664596	11	39	theme	gut	1778:1780	arg1	microbiome					1782:1791	the gut microbiome	1774:1791	the gut microbiome	1774:1791	Moreover, AH modulated the gut microbiome via increasing the abundance of probiotics and decreasing the levels of pathogenic bacteria.					
34664596	1	40	from	supplements	331:341	arg1	medicine					366:373	traditional Chinese medicine	346:373	traditional Chinese medicine	346:373	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	0	41	theme	polysaccharides	32:46	arg1	Supplementation					0:14	Supplementation	0:14	Supplementation of Alhagi honey polysaccharides	0:46	Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice.					
34664596	9	42	theme	gene	1462:1465	arg1	sequencing					1467:1476	16S rDNA gene sequencing	1453:1476	16S rDNA gene sequencing	1453:1476	Furthermore, 16S rDNA gene sequencing was used to monitor the dynamic changes in the gut microbiota.					
34664596	7	43	theme	samples	1186:1192	arg1	cells					1164:1168	IgA+ cells	1159:1168	IgA+ cells of AH-treatment samples	1159:1192	An obvious increase in the ratio of IgA+ cells of AH-treatment samples in the lamina propria was also detected by immunohistochemical staining.					
34664596	3	44	theme	200	615:617	arg1	mg					619:620	mg	619:620	mg	619:620	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	5	45	theme	TNF-α	924:928	arg1	secretion					939:947	TNF-α cytokine secretion	924:947	TNF-α cytokine secretion	924:947	Simultaneously, AH also significantly stimulated IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, and TNF-α cytokine secretion as compared to the control samples.					
34664596	11	46	theme	pathogenic	1865:1874	arg1	bacteria					1876:1883	pathogenic bacteria	1865:1883	pathogenic bacteria	1865:1883	Moreover, AH modulated the gut microbiome via increasing the abundance of probiotics and decreasing the levels of pathogenic bacteria.					
34664596	7	47	theme	IgA+	1159:1162	arg1	cells					1164:1168	IgA+ cells	1159:1168	IgA+ cells of AH-treatment samples	1159:1192	An obvious increase in the ratio of IgA+ cells of AH-treatment samples in the lamina propria was also detected by immunohistochemical staining.					
34664596	3	48	theme	400	628:630	arg1	mg					632:633	mg	632:633	mg	632:633	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	4	49	theme	intestinal	732:741	arg1	function					751:758	the intestinal barrier function	728:758	the intestinal barrier function	728:758	Results indicated an improvement in the intestinal barrier function through increases in secretory immunoglobulin A (sIgA) and β-defensins.					
34664596	1	50	theme	Alhagi	222:227	arg1	honey					229:233	Alhagi honey	222:233	Alhagi honey	222:233	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	3	51	theme	mg	649:650	arg1	kg-1					652:655	800 mg kg-1	645:655	800 mg kg-1	645:655	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	3	51	theme	mg	649:650	arg1	dosages					606:612	three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1	600:655	dosages	606:612	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	12	52	theme	gut	2025:2027	arg1	microbiome					2029:2038	the gut microbiome	2021:2038	the gut microbiome	2021:2038	In summary, these results indicated that AH could be used as a prebiotic to enhance murine intestinal mucosal immunity and to modulate the gut microbiome.					
34664596	7	53	from	increase	1134:1141	arg1	ratio					1150:1154	the ratio	1146:1154	the ratio of IgA+ cells of AH-treatment samples in the lamina propria	1146:1214	An obvious increase in the ratio of IgA+ cells of AH-treatment samples in the lamina propria was also detected by immunohistochemical staining.					
34664596	11	54	theme	bacteria	1876:1883	arg1	levels					1855:1860	the levels	1851:1860	the levels of pathogenic bacteria	1851:1883	Moreover, AH modulated the gut microbiome via increasing the abundance of probiotics and decreasing the levels of pathogenic bacteria.					
34664596	8	55	dep	CD3+	1284:1287	arg1	CD8+					1299:1302	CD8+	1299:1302	CD8+	1299:1302	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	8	55	dep	CD3+	1284:1287	arg1	CD4+					1290:1293	CD4+	1290:1293	CD4+	1290:1293	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	3	56	theme	saline	661:666	arg1	administration					578:591	daily intragastric administration	559:591	daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days	559:689	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	10	57	theme	Shannon	1608:1614	arg1	indexes					1597:1603	the indexes	1593:1603	the indexes of Shannon	1593:1614	The result revealed that AH significantly increased the indexes of Shannon and obviously decreased the indexes of Simpson, suggesting the enhancement of the diversity and richness of the intestinal microbiome.					
34664596	6	58	theme	intraepithelial	1064:1078	arg1	lymphocytes					1080:1090	intraepithelial lymphocytes	1064:1090	intraepithelial lymphocytes (IELs)	1064:1097	Moreover, hematoxylin and eosin staining showed that AH enhanced the number of intraepithelial lymphocytes (IELs) in the small intestine.					
34664596	6	58	theme	intraepithelial	1064:1078	arg1	IELs					1093:1096	IELs	1093:1096	IELs	1093:1096	Moreover, hematoxylin and eosin staining showed that AH enhanced the number of intraepithelial lymphocytes (IELs) in the small intestine.					
34664596	12	59	theme	murine	1970:1975	arg1	immunity					1996:2003	murine intestinal mucosal immunity	1970:2003	murine intestinal mucosal immunity	1970:2003	In summary, these results indicated that AH could be used as a prebiotic to enhance murine intestinal mucosal immunity and to modulate the gut microbiome.					
34664596	4	60	theme	immunoglobulin	791:804	arg1	sIgA					809:812	sIgA	809:812	sIgA	809:812	Results indicated an improvement in the intestinal barrier function through increases in secretory immunoglobulin A (sIgA) and β-defensins.					
34664596	4	60	theme	immunoglobulin	791:804	arg1	A					806:806	secretory immunoglobulin A	781:806	secretory immunoglobulin A (sIgA)	781:813	Results indicated an improvement in the intestinal barrier function through increases in secretory immunoglobulin A (sIgA) and β-defensins.					
34664596	12	61	theme	mucosal	1988:1994	arg1	immunity					1996:2003	murine intestinal mucosal immunity	1970:2003	murine intestinal mucosal immunity	1970:2003	In summary, these results indicated that AH could be used as a prebiotic to enhance murine intestinal mucosal immunity and to modulate the gut microbiome.					
34664596	0	62	theme	intestinal	134:143	arg1	flora					145:149	intestinal flora	134:149	intestinal flora	134:149	Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice.					
34664596	2	63	theme	gut	525:527	arg1	microbiome					529:538	the gut microbiome	521:538	the gut microbiome	521:538	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	8	64	theme	mesenteric	1320:1329	arg1	nodes					1337:1341	mesenteric lymph nodes	1320:1341	mesenteric lymph nodes	1320:1341	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	1	65	theme	main	197:200	arg1	component					209:217	a main active component	195:217	a main active component of Alhagi honey	195:233	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	65	theme	main	197:200	arg1	polysaccharides					173:187	Alhagi honey polysaccharides	160:187	Alhagi honey polysaccharides (AH)	160:192	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	66	theme	years	392:396	arg1	thousands					379:387	thousands	379:387	thousands of years	379:396	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	3	67	theme	daily	559:563	arg1	administration					578:591	daily intragastric administration	559:591	daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days	559:689	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	0	68	from	structure	121:129	arg1	mice					154:157	mice	154:157	mice	154:157	Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice.					
34664596	10	69	theme	diversity	1698:1706	arg1	enhancement					1679:1689	the enhancement	1675:1689	the enhancement of the diversity and richness of the intestinal microbiome	1675:1748	The result revealed that AH significantly increased the indexes of Shannon and obviously decreased the indexes of Simpson, suggesting the enhancement of the diversity and richness of the intestinal microbiome.					
34664596	8	70	from	nodes	1337:1341	arg1	AH-treatment					1370:1381	the AH-treatment	1366:1381	the AH-treatment	1366:1381	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	3	71	theme	AH	596:597	arg1	administration					578:591	daily intragastric administration	559:591	daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days	559:689	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	2	72	theme	intestinal	467:476	arg1	function					493:500	murine intestinal mucosal immune function	460:500	murine intestinal mucosal immune function	460:500	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	0	73	theme	intestinal	86:95	arg1	immunity					97:104	the intestinal immunity	82:104	the intestinal immunity regulating the structure of intestinal flora in mice	82:157	Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice.					
34664596	4	74	from	improvement	713:723	arg1	function					751:758	the intestinal barrier function	728:758	the intestinal barrier function	728:758	Results indicated an improvement in the intestinal barrier function through increases in secretory immunoglobulin A (sIgA) and β-defensins.					
34664596	1	75	theme	Alhagi	160:165	arg1	AH					190:191	AH	190:191	AH	190:191	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	75	theme	Alhagi	160:165	arg1	polysaccharides					173:187	Alhagi honey polysaccharides	160:187	Alhagi honey polysaccharides (AH)	160:192	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	75	theme	Alhagi	160:165	arg1	supplements					331:341	dietary supplements	323:341	dietary supplements in traditional Chinese medicine	323:373	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	75	theme	Alhagi	160:165	arg1	component					209:217	a main active component	195:217	a main active component of Alhagi honey	195:233	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	2	76	from	effect	444:449	arg1	function					493:500	murine intestinal mucosal immune function	460:500	murine intestinal mucosal immune function	460:500	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	2	76	from	effect	444:449	arg1	composition					506:516	composition	506:516	composition	506:516	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	1	77	theme	traditional	346:356	arg1	medicine					366:373	traditional Chinese medicine	346:373	traditional Chinese medicine	346:373	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	2	78	theme	immune	486:491	arg1	function					493:500	murine intestinal mucosal immune function	460:500	murine intestinal mucosal immune function	460:500	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	7	79	theme	lamina	1201:1206	arg1	propria					1208:1214	the lamina propria	1197:1214	the lamina propria	1197:1214	An obvious increase in the ratio of IgA+ cells of AH-treatment samples in the lamina propria was also detected by immunohistochemical staining.					
34664596	10	80	theme	intestinal	1728:1737	arg1	microbiome					1739:1748	the intestinal microbiome	1724:1748	the intestinal microbiome	1724:1748	The result revealed that AH significantly increased the indexes of Shannon and obviously decreased the indexes of Simpson, suggesting the enhancement of the diversity and richness of the intestinal microbiome.					
34664596	0	81	theme	honey	26:30	arg1	polysaccharides					32:46	Alhagi honey polysaccharides	19:46	Alhagi honey polysaccharides	19:46	Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice.					
34664596	1	82	theme	excellent	257:265	arg1	activities					283:292	excellent pharmacological activities	257:292	excellent pharmacological activities	257:292	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	3	83	theme	mg	619:620	arg1	kg-1					622:625	200 mg kg-1	615:625	200 mg kg-1	615:625	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	3	83	theme	mg	619:620	arg1	dosages					606:612	three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1	600:655	dosages	606:612	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	9	84	from	changes	1510:1516	arg1	microbiota					1529:1538	the gut microbiota	1521:1538	the gut microbiota	1521:1538	Furthermore, 16S rDNA gene sequencing was used to monitor the dynamic changes in the gut microbiota.					
34664596	9	85	theme	rDNA	1457:1460	arg1	sequencing					1467:1476	16S rDNA gene sequencing	1453:1476	16S rDNA gene sequencing	1453:1476	Furthermore, 16S rDNA gene sequencing was used to monitor the dynamic changes in the gut microbiota.					
34664596	7	86	theme	AH-treatment	1173:1184	arg1	samples					1186:1192	AH-treatment samples	1173:1192	AH-treatment samples	1173:1192	An obvious increase in the ratio of IgA+ cells of AH-treatment samples in the lamina propria was also detected by immunohistochemical staining.					
34664596	10	87	theme	richness	1712:1719	arg1	enhancement					1679:1689	the enhancement	1675:1689	the enhancement of the diversity and richness of the intestinal microbiome	1675:1748	The result revealed that AH significantly increased the indexes of Shannon and obviously decreased the indexes of Simpson, suggesting the enhancement of the diversity and richness of the intestinal microbiome.					
34664596	4	88	theme	barrier	743:749	arg1	function					751:758	the intestinal barrier function	728:758	the intestinal barrier function	728:758	Results indicated an improvement in the intestinal barrier function through increases in secretory immunoglobulin A (sIgA) and β-defensins.					
34664596	5	89	theme	cytokine	930:937	arg1	secretion					939:947	TNF-α cytokine secretion	924:947	TNF-α cytokine secretion	924:947	Simultaneously, AH also significantly stimulated IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, and TNF-α cytokine secretion as compared to the control samples.					
34664596	7	90	theme	cells	1164:1168	arg1	ratio					1150:1154	the ratio	1146:1154	the ratio of IgA+ cells of AH-treatment samples in the lamina propria	1146:1214	An obvious increase in the ratio of IgA+ cells of AH-treatment samples in the lamina propria was also detected by immunohistochemical staining.					
34664596	2	91	theme	heath	438:442	arg1	effect					444:449	the heath effect	434:449	the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome	434:538	This study is aimed to investigate the heath effect of AH on murine intestinal mucosal immune function and composition of the gut microbiome.					
34664596	3	92	theme	mg	632:633	arg1	dosages					606:612	three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1	600:655	dosages	606:612	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	3	92	theme	mg	632:633	arg1	kg-1					635:638	400 mg kg-1	628:638	400 mg kg-1	628:638	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	9	93	used	used	1482:1485	arg2	sequencing					1467:1476	16S rDNA gene sequencing	1453:1476	16S rDNA gene sequencing	1453:1476	Furthermore, 16S rDNA gene sequencing was used to monitor the dynamic changes in the gut microbiota.					
34664596	10	94	theme	microbiome	1739:1748	arg1	richness					1712:1719	richness	1712:1719	richness	1712:1719	The result revealed that AH significantly increased the indexes of Shannon and obviously decreased the indexes of Simpson, suggesting the enhancement of the diversity and richness of the intestinal microbiome.					
34664596	10	94	theme	microbiome	1739:1748	arg1	diversity					1698:1706	diversity	1698:1706	diversity	1698:1706	The result revealed that AH significantly increased the indexes of Shannon and obviously decreased the indexes of Simpson, suggesting the enhancement of the diversity and richness of the intestinal microbiome.					
34664596	3	95	dep	dosages	606:612	arg1	kg-1					622:625	200 mg kg-1	615:625	200 mg kg-1	615:625	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	3	95	dep	dosages	606:612	arg1	dosages					606:612	three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1	600:655	dosages	606:612	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	3	95	dep	dosages	606:612	arg1	kg-1					652:655	800 mg kg-1	645:655	800 mg kg-1	645:655	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	3	95	dep	dosages	606:612	arg1	kg-1					635:638	400 mg kg-1	628:638	400 mg kg-1	628:638	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	6	96	theme	eosin	1011:1015	arg1	staining					1017:1024	hematoxylin and eosin staining	995:1024	hematoxylin and eosin staining	995:1024	Moreover, hematoxylin and eosin staining showed that AH enhanced the number of intraepithelial lymphocytes (IELs) in the small intestine.					
34664596	3	97	theme	800	645:647	arg1	mg					649:650	mg	649:650	mg	649:650	ICR mice received daily intragastric administration of AH (three dosages, 200 mg kg-1, 400 mg kg-1, and 800 mg kg-1) or saline for 7 consecutive days.					
34664596	8	98	theme	T-cell	1304:1309	arg1	higher					1401:1406	higher	1401:1406	higher	1401:1406	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	8	98	theme	T-cell	1304:1309	arg1	ratio					1311:1315	the CD3+, CD4+ and CD8+ T-cell ratio	1280:1315	the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment	1280:1381	In addition, the CD3+, CD4+ and CD8+ T-cell ratio in mesenteric lymph nodes and Peyer's patches in the AH-treatment was significantly higher than that in the control group.					
34664596	1	99	theme	honey	229:233	arg1	component					209:217	a main active component	195:217	a main active component of Alhagi honey	195:233	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
34664596	1	99	theme	honey	229:233	arg1	polysaccharides					173:187	Alhagi honey polysaccharides	160:187	Alhagi honey polysaccharides (AH)	160:192	Alhagi honey polysaccharides (AH), a main active component of Alhagi honey, are known to possess excellent pharmacological activities and have been widely used as dietary supplements in traditional Chinese medicine for thousands of years.					
31641758	1	0	theme	units	355:359	arg1	2					336:336	2	336:336	2	336:336	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	1	0	theme	units	355:359	arg1	units					355:359	the repeating units	341:359	the repeating units that constitute the full-length native structure	341:408	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	1	1	theme	type	269:272	arg1	polysaccharide					300:313	the type III group B Streptococcus polysaccharide	265:313	the type III group B Streptococcus polysaccharide	265:313	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	3	2	theme	full-length	713:723	arg1	polysaccharide					732:745	the full-length native polysaccharide	709:745	the full-length native polysaccharide	709:745	We show that highly glycosylated short oligosaccharide conjugates elicit functional immune responses comparable to those of the full-length native polysaccharide.					
31641758	0	3	theme	Minimal	95:101	arg1	Epitope					114:120	a Minimal Protective Epitope	93:120	a Minimal Protective Epitope	93:120	Evaluation of Immune Responses to Group B Streptococcus Type III Oligosaccharides Containing a Minimal Protective Epitope.					
31641758	3	4	theme	native	725:730	arg1	polysaccharide					732:745	the full-length native polysaccharide	709:745	the full-length native polysaccharide	709:745	We show that highly glycosylated short oligosaccharide conjugates elicit functional immune responses comparable to those of the full-length native polysaccharide.					
31641758	1	5	theme	group	278:282	arg1	polysaccharide					300:313	the type III group B Streptococcus polysaccharide	265:313	the type III group B Streptococcus polysaccharide	265:313	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	1	6	theme	B	284:284	arg1	polysaccharide					300:313	the type III group B Streptococcus polysaccharide	265:313	the type III group B Streptococcus polysaccharide	265:313	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	1	7	theme	full-length	381:391	arg1	structure					400:408	the full-length native structure	377:408	the full-length native structure	377:408	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	4	8	theme	glycoconjugate	812:825	arg1	vaccines					827:834	well-defined glycoconjugate vaccines	799:834	well-defined glycoconjugate vaccines based on short synthetic oligosaccharides	799:876	The obtained results pave the way to the design of well-defined glycoconjugate vaccines based on short synthetic oligosaccharides.					
31641758	2	9	theme	current	418:424	arg1	study					426:430	the current study	414:430	the current study	414:430	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
31641758	0	10	theme	Protective	103:112	arg1	Epitope					114:120	a Minimal Protective Epitope	93:120	a Minimal Protective Epitope	93:120	Evaluation of Immune Responses to Group B Streptococcus Type III Oligosaccharides Containing a Minimal Protective Epitope.					
31641758	1	11	theme	native	393:398	arg1	structure					400:408	the full-length native structure	377:408	the full-length native structure	377:408	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	2	12	theme	novel	481:485	arg1	vaccine					487:493	a novel vaccine	479:493	a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein	479:582	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
31641758	4	13	theme	well-defined	799:810	arg1	vaccines					827:834	well-defined glycoconjugate vaccines	799:834	well-defined glycoconjugate vaccines based on short synthetic oligosaccharides	799:876	The obtained results pave the way to the design of well-defined glycoconjugate vaccines based on short synthetic oligosaccharides.					
31641758	2	14	theme	discovery	459:467	arg1	advantage					441:449	advantage	441:449	advantage of this discovery	441:467	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
31641758	1	15	theme	Streptococcus	286:298	arg1	polysaccharide					300:313	the type III group B Streptococcus polysaccharide	265:313	the type III group B Streptococcus polysaccharide	265:313	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	1	16	theme	monoclonal	195:204	arg1	antibody					206:213	a monoclonal antibody	193:213	a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide	193:313	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	0	17	theme	Responses	21:29	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Immune Responses to Group B Streptococcus Type III Oligosaccharides	0:80	Evaluation of Immune Responses to Group B Streptococcus Type III Oligosaccharides Containing a Minimal Protective Epitope.					
31641758	4	18	theme	short	845:849	arg1	oligosaccharides					861:876	short synthetic oligosaccharides	845:876	short synthetic oligosaccharides	845:876	The obtained results pave the way to the design of well-defined glycoconjugate vaccines based on short synthetic oligosaccharides.					
31641758	3	19	gly	glycosylated	605:616	arg1	conjugates					640:649	highly glycosylated short oligosaccharide conjugates	598:649	highly glycosylated short oligosaccharide conjugates	598:649	We show that highly glycosylated short oligosaccharide conjugates elicit functional immune responses comparable to those of the full-length native polysaccharide.					
31641758	0	20	theme	Immune	14:19	arg1	Responses					21:29	Immune Responses	14:29	Immune Responses to Group B Streptococcus Type III Oligosaccharides	14:80	Evaluation of Immune Responses to Group B Streptococcus Type III Oligosaccharides Containing a Minimal Protective Epitope.					
31641758	1	21	theme	representative	215:228	arg1	antibody					206:213	a monoclonal antibody	193:213	a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide	193:313	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	0	22	theme	Group	34:38	arg1	Oligosaccharides					65:80	Group B Streptococcus Type III Oligosaccharides	34:80	Group B Streptococcus Type III Oligosaccharides	34:80	Evaluation of Immune Responses to Group B Streptococcus Type III Oligosaccharides Containing a Minimal Protective Epitope.					
31641758	2	23	theme	epitope	555:561	arg1	presentation					516:527	multivalent presentation	504:527	multivalent presentation of the identified minimal epitope on a carrier protein	504:582	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
31641758	3	24	theme	comparable	686:695	arg1	responses					676:684	functional immune responses	658:684	functional immune responses comparable to those of the full-length native polysaccharide	658:745	We show that highly glycosylated short oligosaccharide conjugates elicit functional immune responses comparable to those of the full-length native polysaccharide.					
31641758	2	25	from	protein	576:582	arg1	presentation					516:527	multivalent presentation	504:527	multivalent presentation of the identified minimal epitope on a carrier protein	504:582	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
31641758	2	26	theme	minimal	547:553	arg1	epitope					555:561	the identified minimal epitope	532:561	the identified minimal epitope on a carrier protein	532:582	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
31641758	3	27	theme	functional	658:667	arg1	responses					676:684	functional immune responses	658:684	functional immune responses comparable to those of the full-length native polysaccharide	658:745	We show that highly glycosylated short oligosaccharide conjugates elicit functional immune responses comparable to those of the full-length native polysaccharide.					
31641758	3	28	theme	glycosylated	605:616	arg1	conjugates					640:649	highly glycosylated short oligosaccharide conjugates	598:649	highly glycosylated short oligosaccharide conjugates	598:649	We show that highly glycosylated short oligosaccharide conjugates elicit functional immune responses comparable to those of the full-length native polysaccharide.					
31641758	0	29	theme	Streptococcus	42:54	arg1	Oligosaccharides					65:80	Group B Streptococcus Type III Oligosaccharides	34:80	Group B Streptococcus Type III Oligosaccharides	34:80	Evaluation of Immune Responses to Group B Streptococcus Type III Oligosaccharides Containing a Minimal Protective Epitope.					
31641758	2	30	theme	identified	536:545	arg1	epitope					555:561	the identified minimal epitope	532:561	the identified minimal epitope on a carrier protein	532:582	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
31641758	3	31	theme	immune	669:674	arg1	responses					676:684	functional immune responses	658:684	functional immune responses comparable to those of the full-length native polysaccharide	658:745	We show that highly glycosylated short oligosaccharide conjugates elicit functional immune responses comparable to those of the full-length native polysaccharide.					
31641758	4	32	theme	obtained	752:759	arg1	results					761:767	The obtained results	748:767	The obtained results	748:767	The obtained results pave the way to the design of well-defined glycoconjugate vaccines based on short synthetic oligosaccharides.					
31641758	0	33	theme	B	40:40	arg1	Oligosaccharides					65:80	Group B Streptococcus Type III Oligosaccharides	34:80	Group B Streptococcus Type III Oligosaccharides	34:80	Evaluation of Immune Responses to Group B Streptococcus Type III Oligosaccharides Containing a Minimal Protective Epitope.					
31641758	1	34	theme	Recent	123:128	arg1	studies					141:147	Recent structural studies	123:147	Recent structural studies	123:147	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	2	35	from	presentation	516:527	arg1	protein					576:582	a carrier protein	566:582	a carrier protein	566:582	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
31641758	4	36	theme	synthetic	851:859	arg1	oligosaccharides					861:876	short synthetic oligosaccharides	845:876	short synthetic oligosaccharides	845:876	The obtained results pave the way to the design of well-defined glycoconjugate vaccines based on short synthetic oligosaccharides.					
31641758	1	37	theme	structural	130:139	arg1	studies					141:147	Recent structural studies	123:147	Recent structural studies	123:147	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	1	38	theme	protective	237:246	arg1	response					248:255	the protective response	233:255	the protective response against the type III group B Streptococcus polysaccharide	233:313	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	2	39	theme	carrier	568:574	arg1	protein					576:582	a carrier protein	566:582	a carrier protein	566:582	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
31641758	4	40	theme	vaccines	827:834	arg1	design					789:794	the design	785:794	the design of well-defined glycoconjugate vaccines based on short synthetic oligosaccharides	785:876	The obtained results pave the way to the design of well-defined glycoconjugate vaccines based on short synthetic oligosaccharides.					
31641758	1	41	theme	response	248:255	arg1	representative					215:228	representative	215:228	representative	215:228	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	2	42	theme	multivalent	504:514	arg1	presentation					516:527	multivalent presentation	504:527	multivalent presentation of the identified minimal epitope on a carrier protein	504:582	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
31641758	3	43	theme	short	618:622	arg1	conjugates					640:649	highly glycosylated short oligosaccharide conjugates	598:649	highly glycosylated short oligosaccharide conjugates	598:649	We show that highly glycosylated short oligosaccharide conjugates elicit functional immune responses comparable to those of the full-length native polysaccharide.					
31641758	0	44	theme	Type	56:59	arg1	Oligosaccharides					65:80	Group B Streptococcus Type III Oligosaccharides	34:80	Group B Streptococcus Type III Oligosaccharides	34:80	Evaluation of Immune Responses to Group B Streptococcus Type III Oligosaccharides Containing a Minimal Protective Epitope.					
31641758	1	45	theme	repeating	345:353	arg1	units					355:359	the repeating units	341:359	the repeating units that constitute the full-length native structure	341:408	Recent structural studies demonstrated that the epitope recognized by a monoclonal antibody representative of the protective response against the type III group B Streptococcus polysaccharide was comprised within 2 of the repeating units that constitute the full-length native structure.					
31641758	3	46	theme	oligosaccharide	624:638	arg1	conjugates					640:649	highly glycosylated short oligosaccharide conjugates	598:649	highly glycosylated short oligosaccharide conjugates	598:649	We show that highly glycosylated short oligosaccharide conjugates elicit functional immune responses comparable to those of the full-length native polysaccharide.					
31641758	2	47	from	epitope	555:561	arg1	protein					576:582	a carrier protein	566:582	a carrier protein	566:582	In the current study, we took advantage of this discovery to design a novel vaccine based on multivalent presentation of the identified minimal epitope on a carrier protein.					
34510906	0	0	from	Effect	18:23	arg1	Formation					80:88	Formation	80:88	Formation of Polysaccharide-Based Hybrid Hydrogels	80:129	Understanding the Effect of Conformational Rigidity on Rheological Behavior and Formation of Polysaccharide-Based Hybrid Hydrogels.					
34510906	0	0	from	Effect	18:23	arg1	Behavior					67:74	Rheological Behavior	55:74	Rheological Behavior	55:74	Understanding the Effect of Conformational Rigidity on Rheological Behavior and Formation of Polysaccharide-Based Hybrid Hydrogels.					
34510906	1	1	theme	macroscopic	177:187	arg1	properties					201:210	macroscopic rheological properties	177:210	macroscopic rheological properties	177:210	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	4	2	theme	Xanthan	631:637	arg1	gum					639:641	Xanthan gum	631:641	Xanthan gum	631:641	Xanthan gum exhibits a much stronger shear thinning and more solidlike behavior compared to hyaluronic acid, owing to its network/quaternary structure.					
34510906	1	3	theme	rheological	189:199	arg1	properties					201:210	macroscopic rheological properties	177:210	macroscopic rheological properties	177:210	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	1	4	theme	hyaluronic	282:291	arg1	polysaccharides					241:255	two model polysaccharides	231:255	two model polysaccharides	231:255	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	1	4	theme	hyaluronic	282:291	arg1	acid					293:296	hyaluronic acid	282:296	hyaluronic acid	282:296	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	0	5	theme	Hybrid	114:119	arg1	Hydrogels					121:129	Polysaccharide-Based Hybrid Hydrogels	93:129	Polysaccharide-Based Hybrid Hydrogels	93:129	Understanding the Effect of Conformational Rigidity on Rheological Behavior and Formation of Polysaccharide-Based Hybrid Hydrogels.					
34510906	3	6	theme	flexible	556:563	arg1	conformation					574:585	a relatively flexible tertiary conformation	543:585	a relatively flexible tertiary conformation due to its secondary random coil structure	543:628	In comparison, hyaluronic acid possesses a relatively flexible tertiary conformation due to its secondary random coil structure.					
34510906	3	7	theme	random	608:613	arg1	structure					620:628	its secondary random coil structure	594:628	its secondary random coil structure	594:628	In comparison, hyaluronic acid possesses a relatively flexible tertiary conformation due to its secondary random coil structure.					
34510906	6	8	theme	xanthan	1062:1068	arg1	gum					1070:1072	xanthan gum	1062:1072	xanthan gum	1062:1072	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	0	9	theme	Polysaccharide-Based	93:112	arg1	Hydrogels					121:129	Polysaccharide-Based Hybrid Hydrogels	93:129	Polysaccharide-Based Hybrid Hydrogels	93:129	Understanding the Effect of Conformational Rigidity on Rheological Behavior and Formation of Polysaccharide-Based Hybrid Hydrogels.					
34510906	5	10	with	gum	882:884	arg1	resistance					898:907	better resistance	891:907	better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid	891:1019	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	5	11	theme	urea	958:961	arg1	addition					963:970	salt and/or urea addition	946:970	salt and/or urea addition	946:970	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	5	11	theme	urea	958:961	arg1	change					985:990	temperature change	973:990	temperature change	973:990	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	3	12	theme	hyaluronic	517:526	arg1	acid					528:531	hyaluronic acid	517:531	hyaluronic acid	517:531	In comparison, hyaluronic acid possesses a relatively flexible tertiary conformation due to its secondary random coil structure.					
34510906	5	13	theme	tertiary	793:800	arg1	structure					802:810	The rigid tertiary structure	783:810	The rigid tertiary structure	783:810	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	4	14	theme	network/quaternary	753:770	arg1	structure					772:780	its network/quaternary structure	749:780	its network/quaternary structure	749:780	Xanthan gum exhibits a much stronger shear thinning and more solidlike behavior compared to hyaluronic acid, owing to its network/quaternary structure.					
34510906	6	15	theme	gelation	1161:1168	arg1	possible					1194:1201	possible	1194:1201	possible	1194:1201	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	6	15	theme	gelation	1161:1168	arg1	technique					1170:1178	the vapor-induced gelation technique	1143:1178	the vapor-induced gelation technique	1143:1178	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	6	16	theme	tertiary	1243:1250	arg1	conformation					1252:1263	its flexible tertiary conformation	1230:1263	its flexible tertiary conformation under similar conditions	1230:1288	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	2	17	theme	tertiary	423:430	arg1	structures					432:441	the tertiary structures	419:441	the tertiary structures	419:441	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	0	18	theme	Hydrogels	121:129	arg1	Formation					80:88	Formation	80:88	Formation of Polysaccharide-Based Hybrid Hydrogels	80:129	Understanding the Effect of Conformational Rigidity on Rheological Behavior and Formation of Polysaccharide-Based Hybrid Hydrogels.					
34510906	0	18	theme	Hydrogels	121:129	arg1	Behavior					67:74	Rheological Behavior	55:74	Rheological Behavior	55:74	Understanding the Effect of Conformational Rigidity on Rheological Behavior and Formation of Polysaccharide-Based Hybrid Hydrogels.					
34510906	3	19	contain	possesses	533:541	arg1	acid					528:531	hyaluronic acid	517:531	hyaluronic acid	517:531	In comparison, hyaluronic acid possesses a relatively flexible tertiary conformation due to its secondary random coil structure.					
34510906	3	19	contain	possesses	533:541	arg2	conformation					574:585	a relatively flexible tertiary conformation	543:585	a relatively flexible tertiary conformation due to its secondary random coil structure	543:628	In comparison, hyaluronic acid possesses a relatively flexible tertiary conformation due to its secondary random coil structure.					
34510906	2	20	theme	Xanthan	299:305	arg1	gum					307:309	Xanthan gum	299:309	Xanthan gum	299:309	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	6	21	theme	vapor-induced	1147:1159	arg1	possible					1194:1201	possible	1194:1201	possible	1194:1201	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	6	21	theme	vapor-induced	1147:1159	arg1	technique					1170:1178	the vapor-induced gelation technique	1143:1178	the vapor-induced gelation technique	1143:1178	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	2	22	theme	network/quaternary	472:489	arg1	structure					491:499	a network/quaternary structure	470:499	a network/quaternary structure	470:499	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	2	23	theme	due	345:347	arg1	conformation					332:343	a rigid tertiary conformation	315:343	a rigid tertiary conformation due to its ordered double-helical structure	315:387	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	4	24	theme	hyaluronic	723:732	arg1	acid					734:737	hyaluronic acid	723:737	hyaluronic acid	723:737	Xanthan gum exhibits a much stronger shear thinning and more solidlike behavior compared to hyaluronic acid, owing to its network/quaternary structure.					
34510906	5	25	theme	xanthan	874:880	arg1	gum					882:884	xanthan gum	874:884	xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid	874:1019	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	5	26	dep	addition	963:970	arg1	e.g.					940:943	e.g.	940:943	e.g.	940:943	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	6	27	theme	network/quaternary	1026:1043	arg1	structure					1045:1053	The network/quaternary structure	1022:1053	The network/quaternary structure	1022:1053	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	1	28	from	importance	136:145	arg1	properties					201:210	macroscopic rheological properties	177:210	macroscopic rheological properties	177:210	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	2	29	theme	tertiary	323:330	arg1	conformation					332:343	a rigid tertiary conformation	315:343	a rigid tertiary conformation due to its ordered double-helical structure	315:387	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	5	30	theme	better	891:896	arg1	resistance					898:907	better resistance	891:907	better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid	891:1019	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	2	31	contain	has	311:313	arg2	conformation					332:343	a rigid tertiary conformation	315:343	a rigid tertiary conformation due to its ordered double-helical structure	315:387	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	2	31	contain	has	311:313	arg1	gum					307:309	Xanthan gum	299:309	Xanthan gum	299:309	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	5	32	theme	hyaluronic	1005:1014	arg1	acid					1016:1019	hyaluronic acid	1005:1019	hyaluronic acid	1005:1019	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	2	33	theme	rigid	317:321	arg1	conformation					332:343	a rigid tertiary conformation	315:343	a rigid tertiary conformation due to its ordered double-helical structure	315:387	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	1	34	theme	model	235:239	arg1	acid					293:296	hyaluronic acid	282:296	hyaluronic acid	282:296	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	1	34	theme	model	235:239	arg1	gum					274:276	xanthan gum	266:276	xanthan gum	266:276	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	1	34	theme	model	235:239	arg1	polysaccharides					241:255	two model polysaccharides	231:255	two model polysaccharides	231:255	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	0	35	theme	Rigidity	43:50	arg1	Effect					18:23	the Effect	14:23	the Effect of Conformational Rigidity on Rheological Behavior and Formation of Polysaccharide-Based Hybrid Hydrogels	14:129	Understanding the Effect of Conformational Rigidity on Rheological Behavior and Formation of Polysaccharide-Based Hybrid Hydrogels.					
34510906	5	36	theme	temperature	973:983	arg1	addition					963:970	salt and/or urea addition	946:970	salt and/or urea addition	946:970	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	5	36	theme	temperature	973:983	arg1	change					985:990	temperature change	973:990	temperature change	973:990	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	6	37	theme	electrostatic	1105:1117	arg1	interactions					1119:1130	electrostatic interactions	1105:1130	electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions	1105:1288	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	6	38	with	gels	1082:1085	arg1	chitosan					1092:1099	chitosan	1092:1099	chitosan	1092:1099	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	4	39	theme	solidlike	692:700	arg1	behavior					702:709	more solidlike behavior	687:709	more solidlike behavior	687:709	Xanthan gum exhibits a much stronger shear thinning and more solidlike behavior compared to hyaluronic acid, owing to its network/quaternary structure.					
34510906	0	40	theme	Conformational	28:41	arg1	Rigidity					43:50	Conformational Rigidity	28:50	Conformational Rigidity	28:50	Understanding the Effect of Conformational Rigidity on Rheological Behavior and Formation of Polysaccharide-Based Hybrid Hydrogels.					
34510906	5	41	theme	network/quaternary	834:851	arg1	structure					853:861	a network/quaternary structure	832:861	a network/quaternary structure	832:861	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	5	42	theme	rigid	787:791	arg1	structure					802:810	The rigid tertiary structure	783:810	The rigid tertiary structure	783:810	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	4	43	theme	shear	668:672	arg1	thinning					674:681	a much stronger shear thinning	652:681	a much stronger shear thinning	652:681	Xanthan gum exhibits a much stronger shear thinning and more solidlike behavior compared to hyaluronic acid, owing to its network/quaternary structure.					
34510906	5	44	theme	environmental	917:929	arg1	changes					931:937	environmental changes	917:937	environmental changes (e.g., salt and/or urea addition, temperature change)	917:991	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	0	45	theme	Rheological	55:65	arg1	Behavior					67:74	Rheological Behavior	55:74	Rheological Behavior	55:74	Understanding the Effect of Conformational Rigidity on Rheological Behavior and Formation of Polysaccharide-Based Hybrid Hydrogels.					
34510906	5	46	theme	salt	946:949	arg1	addition					963:970	salt and/or urea addition	946:970	salt and/or urea addition	946:970	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	5	46	theme	salt	946:949	arg1	change					985:990	temperature change	973:990	temperature change	973:990	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	2	47	theme	double-helical	364:377	arg1	structure					379:387	its ordered double-helical structure	352:387	its ordered double-helical structure	352:387	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	2	48	theme	structure	491:499	arg1	formation					457:465	the formation	453:465	the formation of a network/quaternary structure	453:499	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	6	49	theme	due	1223:1225	arg1	acid					1218:1221	hyaluronic acid	1207:1221	hyaluronic acid due to its flexible tertiary conformation under similar conditions	1207:1288	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	2	50	theme	ordered	356:362	arg1	structure					379:387	its ordered double-helical structure	352:387	its ordered double-helical structure	352:387	Xanthan gum has a rigid tertiary conformation due to its ordered double-helical structure, and the interactions between the tertiary structures result in the formation of a network/quaternary structure.					
34510906	3	51	theme	coil	615:618	arg1	structure					620:628	its secondary random coil structure	594:628	its secondary random coil structure	594:628	In comparison, hyaluronic acid possesses a relatively flexible tertiary conformation due to its secondary random coil structure.					
34510906	3	52	theme	secondary	598:606	arg1	structure					620:628	its secondary random coil structure	594:628	its secondary random coil structure	594:628	In comparison, hyaluronic acid possesses a relatively flexible tertiary conformation due to its secondary random coil structure.					
34510906	3	53	theme	due	587:589	arg1	conformation					574:585	a relatively flexible tertiary conformation	543:585	a relatively flexible tertiary conformation due to its secondary random coil structure	543:628	In comparison, hyaluronic acid possesses a relatively flexible tertiary conformation due to its secondary random coil structure.					
34510906	1	54	theme	conformational	150:163	arg1	rigidity					165:172	conformational rigidity	150:172	conformational rigidity	150:172	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	6	55	theme	hyaluronic	1207:1216	arg1	acid					1218:1221	hyaluronic acid	1207:1221	hyaluronic acid due to its flexible tertiary conformation under similar conditions	1207:1288	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	6	56	theme	flexible	1234:1241	arg1	conformation					1252:1263	its flexible tertiary conformation	1230:1263	its flexible tertiary conformation under similar conditions	1230:1288	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	1	57	theme	rigidity	165:172	arg1	importance					136:145	The importance	132:145	The importance of conformational rigidity on macroscopic rheological properties	132:210	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	1	58	theme	xanthan	266:272	arg1	gum					274:276	xanthan gum	266:276	xanthan gum	266:276	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	1	58	theme	xanthan	266:272	arg1	polysaccharides					241:255	two model polysaccharides	231:255	two model polysaccharides	231:255	The importance of conformational rigidity on macroscopic rheological properties was revealed using two model polysaccharides, namely, xanthan gum and hyaluronic acid.					
34510906	5	59	theme	structure	853:861	arg1	structure					802:810	The rigid tertiary structure	783:810	The rigid tertiary structure	783:810	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	5	59	theme	structure	853:861	arg1	presence					820:827	the presence	816:827	the presence of a network/quaternary structure	816:861	The rigid tertiary structure and the presence of a network/quaternary structure also endow xanthan gum with better resistance against environmental changes (e.g., salt and/or urea addition, temperature change) compared to hyaluronic acid.					
34510906	6	60	theme	similar	1271:1277	arg1	conditions					1279:1288	similar conditions	1271:1288	similar conditions	1271:1288	The network/quaternary structure allows xanthan gum to form gels with chitosan via electrostatic interactions when using the vapor-induced gelation technique, which is not possible for hyaluronic acid due to its flexible tertiary conformation under similar conditions.					
34510906	3	61	theme	tertiary	565:572	arg1	conformation					574:585	a relatively flexible tertiary conformation	543:585	a relatively flexible tertiary conformation due to its secondary random coil structure	543:628	In comparison, hyaluronic acid possesses a relatively flexible tertiary conformation due to its secondary random coil structure.					
34510906	4	62	theme	stronger	659:666	arg1	thinning					674:681	a much stronger shear thinning	652:681	a much stronger shear thinning	652:681	Xanthan gum exhibits a much stronger shear thinning and more solidlike behavior compared to hyaluronic acid, owing to its network/quaternary structure.					
33533562	0	0	theme	ultra-performance	95:111	arg1	spectrometry					147:158	ultra-performance liquid chromatography-tandem mass spectrometry	95:158	ultra-performance liquid chromatography-tandem mass spectrometry	95:158	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	3	1	theme	multiple	573:580	arg1	mode					602:605	multiple reaction monitoring mode	573:605	multiple reaction monitoring mode	573:605	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	4	2	dep	deoxyhexoses	673:684	arg1	fucose					697:702	fucose	697:702	fucose	697:702	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	4	2	dep	deoxyhexoses	673:684	arg1	rhamnose					687:694	rhamnose	687:694	rhamnose	687:694	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	3	3	theme	reaction	582:589	arg1	mode					602:605	multiple reaction monitoring mode	573:605	multiple reaction monitoring mode	573:605	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	0	4	theme	chromatography-tandem	120:140	arg1	spectrometry					147:158	ultra-performance liquid chromatography-tandem mass spectrometry	95:158	ultra-performance liquid chromatography-tandem mass spectrometry	95:158	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	4	5	theme	galacturonic	789:800	arg1	acid					783:786	glucuronic acid	772:786	glucuronic acid	772:786	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	4	5	theme	galacturonic	789:800	arg1	acid					802:805	galacturonic acid	789:805	galacturonic acid	789:805	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	4	6	theme	glucuronic	772:781	arg1	acid					783:786	glucuronic acid	772:786	glucuronic acid	772:786	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	4	6	theme	glucuronic	772:781	arg1	acid					802:805	galacturonic acid	789:805	galacturonic acid	789:805	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	0	7	theme	liquid	113:118	arg1	spectrometry					147:158	ultra-performance liquid chromatography-tandem mass spectrometry	95:158	ultra-performance liquid chromatography-tandem mass spectrometry	95:158	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	5	8	theme	1-phenyl-3-methyl-5-pyrazolone	1019:1048	arg1	concentrations					1050:1063	various 1-phenyl-3-methyl-5-pyrazolone concentrations	1011:1063	various 1-phenyl-3-methyl-5-pyrazolone concentrations	1011:1063	Optimization of the derivatization process, especially by using various 1-phenyl-3-methyl-5-pyrazolone concentrations, was studied.					
33533562	9	9	theme	Codium	1561:1566	arg1	fragile					1568:1574	Codium fragile	1561:1574	Codium fragile	1561:1574	Using this method, the monosaccharides comprising the polysaccharides of Codium fragile were determined to be arabinose, galactose, and glucose.					
33533562	4	10	dep	acids	765:769	arg1	acid					783:786	glucuronic acid	772:786	glucuronic acid	772:786	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	4	10	dep	acids	765:769	arg1	acid					802:805	galacturonic acid	789:805	galacturonic acid	789:805	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	2	11	theme	antioxidant	317:327	arg1	properties					351:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties.					
33533562	5	12	theme	derivatization	967:980	arg1	process					982:988	the derivatization process	963:988	the derivatization process	963:988	Optimization of the derivatization process, especially by using various 1-phenyl-3-methyl-5-pyrazolone concentrations, was studied.					
33533562	3	13	theme	liquid	482:487	arg1	spectrometry					516:527	ultra-performance liquid chromatography-tandem mass spectrometry	464:527	ultra-performance liquid chromatography-tandem mass spectrometry	464:527	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	0	14	theme	mass	142:145	arg1	spectrometry					147:158	ultra-performance liquid chromatography-tandem mass spectrometry	95:158	ultra-performance liquid chromatography-tandem mass spectrometry	95:158	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	4	15	dep	pentoses	639:646	arg1	arabinose					657:665	arabinose	657:665	arabinose	657:665	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	4	15	dep	pentoses	639:646	arg1	xylose					649:654	xylose	649:654	xylose	649:654	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	7	16	theme	relative	1234:1241	arg1	deviations					1276:1285	the relative intra-day and inter-day standard deviations	1230:1285	the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively	1230:1338	The spiked recovery was determined to be 91.1-105.7% with the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively.					
33533562	7	17	with	%	1223:1223	arg1	deviations					1276:1285	the relative intra-day and inter-day standard deviations	1230:1285	the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively	1230:1338	The spiked recovery was determined to be 91.1-105.7% with the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively.					
33533562	1	18	theme	Codiales	205:212	arg1	family					214:219	Codiales family	205:219	Codiales family	205:219	Codium fragile is a green alga belonging to Codiales family.					
33533562	7	19	theme	standard	1267:1274	arg1	deviations					1276:1285	the relative intra-day and inter-day standard deviations	1230:1285	the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively	1230:1338	The spiked recovery was determined to be 91.1-105.7% with the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively.					
33533562	7	20	theme	spiked	1176:1181	arg1	recovery					1183:1190	The spiked recovery	1172:1190	The spiked recovery	1172:1190	The spiked recovery was determined to be 91.1-105.7% with the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively.					
33533562	7	20	theme	spiked	1176:1181	arg1	%					1223:1223	91.1-105.7%	1213:1223	91.1-105.7%	1213:1223	The spiked recovery was determined to be 91.1-105.7% with the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively.					
33533562	5	21	dep	Optimization	947:958	arg1	especially					991:1000	especially	991:1000	especially	991:1000	Optimization of the derivatization process, especially by using various 1-phenyl-3-methyl-5-pyrazolone concentrations, was studied.					
33533562	3	22	theme	ultra-performance	464:480	arg1	spectrometry					516:527	ultra-performance liquid chromatography-tandem mass spectrometry	464:527	ultra-performance liquid chromatography-tandem mass spectrometry	464:527	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	0	23	theme	composition	27:37	arg1	Analysis					0:7	Analysis	0:7	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.	0:159	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	4	24	dep	hexoses	712:718	arg1	galactose					739:747	galactose	739:747	galactose	739:747	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	4	24	dep	hexoses	712:718	arg1	glucose					730:736	glucose	730:736	glucose	730:736	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	4	24	dep	hexoses	712:718	arg1	mannose					721:727	mannose	721:727	mannose	721:727	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	5	25	theme	process	982:988	arg1	Optimization					947:958	Optimization	947:958	Optimization	947:958	Optimization of the derivatization process, especially by using various 1-phenyl-3-methyl-5-pyrazolone concentrations, was studied.					
33533562	6	26	theme	squared	1131:1137	arg1	coefficient > 0.995					1151:1169	a squared correlation coefficient > 0.995	1129:1169	a squared correlation coefficient > 0.995	1129:1169	The calibration curves showed good linearity with a squared correlation coefficient > 0.995.					
33533562	0	27	theme	monosaccharide	12:25	arg1	composition					27:37	monosaccharide composition	12:37	monosaccharide composition of water-soluble polysaccharides from Codium fragile	12:90	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	2	28	theme	immunoregulatory	334:349	arg1	properties					351:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties.					
33533562	9	29	theme	fragile	1568:1574	arg1	polysaccharides					1542:1556	the polysaccharides	1538:1556	the polysaccharides of Codium fragile	1538:1574	Using this method, the monosaccharides comprising the polysaccharides of Codium fragile were determined to be arabinose, galactose, and glucose.					
33533562	0	30	theme	water-soluble	42:54	arg1	polysaccharides					56:70	water-soluble polysaccharides	42:70	water-soluble polysaccharides from Codium fragile	42:90	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	8	31	theme	limit	1368:1372	arg1	limit					1345:1349	The limit	1341:1349	The limit of detection and limit of quantification for all 10 monosaccharides	1341:1417	The limit of detection and limit of quantification for all 10 monosaccharides ranged from 0.02 to 0.10 μg/mL and 0.05 to 0.25 μg/mL, respectively.					
33533562	2	32	theme	anti-coagulation	269:284	arg1	properties					351:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties.					
33533562	6	33	theme	correlation	1139:1149	arg1	coefficient > 0.995					1151:1169	a squared correlation coefficient > 0.995	1129:1169	a squared correlation coefficient > 0.995	1129:1169	The calibration curves showed good linearity with a squared correlation coefficient > 0.995.					
33533562	3	34	theme	mass	511:514	arg1	spectrometry					516:527	ultra-performance liquid chromatography-tandem mass spectrometry	464:527	ultra-performance liquid chromatography-tandem mass spectrometry	464:527	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	3	35	theme	monosaccharides	442:456	arg1	analysis					427:434	the analysis	423:434	the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode	423:605	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	7	36	theme	intra-day	1243:1251	arg1	deviations					1276:1285	the relative intra-day and inter-day standard deviations	1230:1285	the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively	1230:1338	The spiked recovery was determined to be 91.1-105.7% with the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively.					
33533562	0	37	from	fragile	84:90	arg1	composition					27:37	monosaccharide composition	12:37	monosaccharide composition of water-soluble polysaccharides from Codium fragile	12:90	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	0	37	from	fragile	84:90	arg1	polysaccharides					56:70	water-soluble polysaccharides	42:70	water-soluble polysaccharides from Codium fragile	42:90	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	3	38	theme	rapid	406:410	arg1	method					412:417	a reliable and rapid method	391:417	a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode	391:605	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	8	39	dep	0.02	1431:1434	arg1	0.10 μg/mL					1439:1448	0.10 μg/mL	1439:1448	0.10 μg/mL	1439:1448	The limit of detection and limit of quantification for all 10 monosaccharides ranged from 0.02 to 0.10 μg/mL and 0.05 to 0.25 μg/mL, respectively.					
33533562	8	39	dep	0.02	1431:1434	arg1	to					1436:1437	to	1436:1437	to	1436:1437	The limit of detection and limit of quantification for all 10 monosaccharides ranged from 0.02 to 0.10 μg/mL and 0.05 to 0.25 μg/mL, respectively.					
33533562	8	39	dep	0.02	1431:1434	arg1	0.05					1454:1457	0.05	1454:1457	0.05	1454:1457	The limit of detection and limit of quantification for all 10 monosaccharides ranged from 0.02 to 0.10 μg/mL and 0.05 to 0.25 μg/mL, respectively.					
33533562	3	40	theme	reliable	393:400	arg1	method					412:417	a reliable and rapid method	391:417	a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode	391:605	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	2	41	theme	alga	259:262	arg1	polysaccharides					235:249	The sulfated polysaccharides	222:249	The sulfated polysaccharides of this alga	222:262	The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties.					
33533562	8	42	theme	quantification	1377:1390	arg1	detection					1354:1362	detection and limit	1354:1372	detection	1354:1362	The limit of detection and limit of quantification for all 10 monosaccharides ranged from 0.02 to 0.10 μg/mL and 0.05 to 0.25 μg/mL, respectively.					
33533562	8	42	theme	quantification	1377:1390	arg1	limit					1368:1372	detection and limit	1354:1372	limit	1368:1372	The limit of detection and limit of quantification for all 10 monosaccharides ranged from 0.02 to 0.10 μg/mL and 0.05 to 0.25 μg/mL, respectively.					
33533562	0	43	theme	polysaccharides	56:70	arg1	composition					27:37	monosaccharide composition	12:37	monosaccharide composition of water-soluble polysaccharides from Codium fragile	12:90	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	1	44	theme	Codium	161:166	arg1	fragile					168:174	Codium fragile	161:174	Codium fragile	161:174	Codium fragile is a green alga belonging to Codiales family.					
33533562	1	44	theme	Codium	161:166	arg1	alga					187:190	a green alga	179:190	a green alga belonging to Codiales family	179:219	Codium fragile is a green alga belonging to Codiales family.					
33533562	2	45	theme	anti-angiogenesis	298:314	arg1	properties					351:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties.					
33533562	3	46	theme	negative	536:543	arg1	ionization					558:567	the negative electrospray ionization	532:567	the negative electrospray ionization	532:567	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	6	47	theme	good	1109:1112	arg1	linearity					1114:1122	good linearity	1109:1122	good linearity	1109:1122	The calibration curves showed good linearity with a squared correlation coefficient > 0.995.					
33533562	2	48	theme	sulfated	226:233	arg1	polysaccharides					235:249	The sulfated polysaccharides	222:249	The sulfated polysaccharides of this alga	222:262	The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties.					
33533562	5	49	theme	various	1011:1017	arg1	concentrations					1050:1063	various 1-phenyl-3-methyl-5-pyrazolone concentrations	1011:1063	various 1-phenyl-3-methyl-5-pyrazolone concentrations	1011:1063	Optimization of the derivatization process, especially by using various 1-phenyl-3-methyl-5-pyrazolone concentrations, was studied.					
33533562	3	50	theme	electrospray	545:556	arg1	ionization					558:567	the negative electrospray ionization	532:567	the negative electrospray ionization	532:567	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	4	51	theme	hexuronic	755:763	arg1	acids					765:769	two hexuronic acids	751:769	two hexuronic acids (glucuronic acid, galacturonic acid)	751:806	Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min.					
33533562	2	52	theme	antiviral	287:295	arg1	properties					351:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties.					
33533562	8	53	theme	detection	1354:1362	arg1	limit					1345:1349	The limit	1341:1349	The limit of detection and limit of quantification for all 10 monosaccharides	1341:1417	The limit of detection and limit of quantification for all 10 monosaccharides ranged from 0.02 to 0.10 μg/mL and 0.05 to 0.25 μg/mL, respectively.					
33533562	3	54	theme	monitoring	591:600	arg1	mode					602:605	multiple reaction monitoring mode	573:605	multiple reaction monitoring mode	573:605	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	3	55	theme	chromatography-tandem	489:509	arg1	spectrometry					516:527	ultra-performance liquid chromatography-tandem mass spectrometry	464:527	ultra-performance liquid chromatography-tandem mass spectrometry	464:527	In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode.					
33533562	2	56	contain	have	264:267	arg2	properties					351:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties	269:360	The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties.					
33533562	2	56	contain	have	264:267	arg1	polysaccharides					235:249	The sulfated polysaccharides	222:249	The sulfated polysaccharides of this alga	222:262	The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties.					
33533562	0	57	theme	Codium	77:82	arg1	fragile					84:90	Codium fragile	77:90	Codium fragile	77:90	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	0	58	from	composition	27:37	arg1	fragile					84:90	Codium fragile	77:90	Codium fragile	77:90	Analysis of monosaccharide composition of water-soluble polysaccharides from Codium fragile by ultra-performance liquid chromatography-tandem mass spectrometry.					
33533562	7	59	theme	inter-day	1257:1265	arg1	deviations					1276:1285	the relative intra-day and inter-day standard deviations	1230:1285	the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively	1230:1338	The spiked recovery was determined to be 91.1-105.7% with the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively.					
33533562	1	60	theme	green	181:185	arg1	fragile					168:174	Codium fragile	161:174	Codium fragile	161:174	Codium fragile is a green alga belonging to Codiales family.					
33533562	1	60	theme	green	181:185	arg1	alga					187:190	a green alga	179:190	a green alga belonging to Codiales family	179:219	Codium fragile is a green alga belonging to Codiales family.					
33533562	6	61	theme	calibration	1083:1093	arg1	curves					1095:1100	The calibration curves	1079:1100	The calibration curves	1079:1100	The calibration curves showed good linearity with a squared correlation coefficient > 0.995.					
32439446	5	0	theme	interesting	1083:1093	arg1	activities					1133:1142	interesting antinociceptive and anti-inflammatory activities	1083:1142	interesting antinociceptive and anti-inflammatory activities	1083:1142	These results showed that the polysaccharide fraction HASP from H. albus leaves has interesting antinociceptive and anti-inflammatory activities.					
32439446	3	1	from	acid	750:753	arg1	mice					758:761	mice	758:761	mice	758:761	HASP was able to promote antinociception in formalin-induced (second phase) and on glutamate-induced nociception tests, besides reducing the number of abdominal contortions induced by acetic acid in mice.					
32439446	1	2	theme	yellow	163:168	arg1	ipê					170:172	yellow ipê	163:172	yellow ipê	163:172	Handroanthus albus, commonly known as yellow ipê, is a native and widely distributed tree in Brazil.					
32439446	4	3	theme	carrageenan-induced	962:980	arg1	model					992:996	the carrageenan-induced paw edema model	958:996	the carrageenan-induced paw edema model	958:996	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	4	4	theme	paw	982:984	arg1	model					992:996	the carrageenan-induced paw edema model	958:996	the carrageenan-induced paw edema model	958:996	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	0	5	theme	antinociceptive	75:89	arg1	activities					113:122	antinociceptive and anti-inflammatory activities	75:122	antinociceptive and anti-inflammatory activities	75:122	A polysaccharide fraction from Handroanthus albus (yellow ipê) leaves with antinociceptive and anti-inflammatory activities.					
32439446	4	6	theme	anti-edematogenic	866:882	arg1	activity					884:891	anti-edematogenic activity	866:891	anti-edematogenic activity	866:891	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	5	7	dep	leaves	1072:1077	arg1	has					1079:1081	has	1079:1081	leaves has interesting antinociceptive and anti-inflammatory activities	1072:1142	These results showed that the polysaccharide fraction HASP from H. albus leaves has interesting antinociceptive and anti-inflammatory activities.					
32439446	2	8	from	proportion	547:556	arg1	present					526:532	present	526:532	present	526:532	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	2	9	theme	other	490:494	arg1	polysaccharides					496:510	other polysaccharides	490:510	other polysaccharides	490:510	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	3	10	theme	abdominal	710:718	arg1	contortions					720:730	abdominal contortions	710:730	abdominal contortions induced by acetic acid in mice	710:761	HASP was able to promote antinociception in formalin-induced (second phase) and on glutamate-induced nociception tests, besides reducing the number of abdominal contortions induced by acetic acid in mice.					
32439446	0	11	theme	anti-inflammatory	95:111	arg1	activities					113:122	antinociceptive and anti-inflammatory activities	75:122	antinociceptive and anti-inflammatory activities	75:122	A polysaccharide fraction from Handroanthus albus (yellow ipê) leaves with antinociceptive and anti-inflammatory activities.					
32439446	0	12	from	albus	44:48	arg1	fraction					17:24	A polysaccharide fraction	0:24	A polysaccharide fraction from Handroanthus albus (yellow ipê)	0:61	A polysaccharide fraction from Handroanthus albus (yellow ipê) leaves with antinociceptive and anti-inflammatory activities.					
32439446	4	13	theme	edema	986:990	arg1	model					992:996	the carrageenan-induced paw edema model	958:996	the carrageenan-induced paw edema model	958:996	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	0	14	theme	polysaccharide	2:15	arg1	fraction					17:24	A polysaccharide fraction	0:24	A polysaccharide fraction from Handroanthus albus (yellow ipê)	0:61	A polysaccharide fraction from Handroanthus albus (yellow ipê) leaves with antinociceptive and anti-inflammatory activities.					
32439446	4	15	theme	peritoneal	837:846	arg1	cavity					848:853	the peritoneal cavity	833:853	the peritoneal cavity	833:853	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	2	16	located	present	526:532	arg2	polysaccharides					496:510	other polysaccharides	490:510	other polysaccharides	490:510	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	2	16	located	present	526:532	arg1	proportion					547:556	a smaller proportion	537:556	a smaller proportion	537:556	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	3	17	theme	acetic	743:748	arg1	acid					750:753	acetic acid	743:753	acetic acid in mice	743:761	HASP was able to promote antinociception in formalin-induced (second phase) and on glutamate-induced nociception tests, besides reducing the number of abdominal contortions induced by acetic acid in mice.					
32439446	4	18	theme	myeloperoxidase	930:944	arg1	activity					946:953	myeloperoxidase activity	930:953	myeloperoxidase activity	930:953	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	5	19	theme	fraction	1044:1051	arg1	HASP					1053:1056	the polysaccharide fraction HASP	1025:1056	the polysaccharide fraction HASP from H. albus	1025:1070	These results showed that the polysaccharide fraction HASP from H. albus leaves has interesting antinociceptive and anti-inflammatory activities.					
32439446	1	20	theme	native	180:185	arg1	albus					138:142	Handroanthus albus	125:142	Handroanthus albus	125:142	Handroanthus albus, commonly known as yellow ipê, is a native and widely distributed tree in Brazil.					
32439446	1	20	theme	native	180:185	arg1	tree					210:213	a native and widely distributed tree	178:213	a native and widely distributed tree in Brazil	178:223	Handroanthus albus, commonly known as yellow ipê, is a native and widely distributed tree in Brazil.					
32439446	3	21	theme	contortions	720:730	arg1	number					700:705	the number	696:705	the number of abdominal contortions induced by acetic acid in mice	696:761	HASP was able to promote antinociception in formalin-induced (second phase) and on glutamate-induced nociception tests, besides reducing the number of abdominal contortions induced by acetic acid in mice.					
32439446	5	22	theme	antinociceptive	1095:1109	arg1	activities					1133:1142	interesting antinociceptive and anti-inflammatory activities	1083:1142	interesting antinociceptive and anti-inflammatory activities	1083:1142	These results showed that the polysaccharide fraction HASP from H. albus leaves has interesting antinociceptive and anti-inflammatory activities.					
32439446	2	23	attach	present	526:532	arg2	polysaccharides					496:510	other polysaccharides	490:510	other polysaccharides	490:510	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	2	23	attach	present	526:532	arg1	proportion					547:556	a smaller proportion	537:556	a smaller proportion	537:556	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	4	24	theme	acid-induced	794:805	arg1	infiltration					817:828	acetic acid-induced leukocyte infiltration	787:828	acetic acid-induced leukocyte infiltration in the peritoneal cavity	787:853	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	2	25	theme	type	446:449	arg1	arabinogalactan					454:468	a type II arabinogalactan	444:468	a type II arabinogalactan	444:468	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	5	26	theme	anti-inflammatory	1115:1131	arg1	activities					1133:1142	interesting antinociceptive and anti-inflammatory activities	1083:1142	interesting antinociceptive and anti-inflammatory activities	1083:1142	These results showed that the polysaccharide fraction HASP from H. albus leaves has interesting antinociceptive and anti-inflammatory activities.					
32439446	4	27	theme	acetic	787:792	arg1	infiltration					817:828	acetic acid-induced leukocyte infiltration	787:828	acetic acid-induced leukocyte infiltration in the peritoneal cavity	787:853	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	4	28	from	infiltration	817:828	arg1	cavity					848:853	the peritoneal cavity	833:853	the peritoneal cavity	833:853	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	2	29	theme	NMR	385:387	arg1	spectroscopy					389:400	NMR spectroscopy	385:400	NMR spectroscopy	385:400	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	0	30	theme	yellow	51:56	arg1	albus					44:48	Handroanthus albus	31:48	Handroanthus albus (yellow ipê)	31:61	A polysaccharide fraction from Handroanthus albus (yellow ipê) leaves with antinociceptive and anti-inflammatory activities.					
32439446	0	30	theme	yellow	51:56	arg1	ipê					58:60	yellow ipê	51:60	yellow ipê	51:60	A polysaccharide fraction from Handroanthus albus (yellow ipê) leaves with antinociceptive and anti-inflammatory activities.					
32439446	3	31	theme	second	621:626	arg1	phase					628:632	second phase	621:632	second phase	621:632	HASP was able to promote antinociception in formalin-induced (second phase) and on glutamate-induced nociception tests, besides reducing the number of abdominal contortions induced by acetic acid in mice.					
32439446	3	31	theme	second	621:626	arg1	formalin-induced					603:618	formalin-induced	603:618	formalin-induced	603:618	HASP was able to promote antinociception in formalin-induced (second phase) and on glutamate-induced nociception tests, besides reducing the number of abdominal contortions induced by acetic acid in mice.					
32439446	1	32	from	tree	210:213	arg1	Brazil					218:223	Brazil	218:223	Brazil	218:223	Handroanthus albus, commonly known as yellow ipê, is a native and widely distributed tree in Brazil.					
32439446	2	33	theme	monosaccharide	310:323	arg1	composition					325:335	monosaccharide composition	310:335	monosaccharide composition	310:335	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	3	34	theme	glutamate-induced	642:658	arg1	tests					672:676	glutamate-induced nociception tests	642:676	glutamate-induced nociception tests	642:676	HASP was able to promote antinociception in formalin-induced (second phase) and on glutamate-induced nociception tests, besides reducing the number of abdominal contortions induced by acetic acid in mice.					
32439446	4	35	theme	leukocyte	807:815	arg1	infiltration					817:828	acetic acid-induced leukocyte infiltration	787:828	acetic acid-induced leukocyte infiltration in the peritoneal cavity	787:853	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	1	36	theme	Handroanthus	125:136	arg1	albus					138:142	Handroanthus albus	125:142	Handroanthus albus	125:142	Handroanthus albus, commonly known as yellow ipê, is a native and widely distributed tree in Brazil.					
32439446	1	36	theme	Handroanthus	125:136	arg1	tree					210:213	a native and widely distributed tree	178:213	a native and widely distributed tree in Brazil	178:223	Handroanthus albus, commonly known as yellow ipê, is a native and widely distributed tree in Brazil.					
32439446	2	37	theme	smaller	539:545	arg1	proportion					547:556	a smaller proportion	537:556	a smaller proportion	537:556	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	3	38	theme	nociception	660:670	arg1	tests					672:676	glutamate-induced nociception tests	642:676	glutamate-induced nociception tests	642:676	HASP was able to promote antinociception in formalin-induced (second phase) and on glutamate-induced nociception tests, besides reducing the number of abdominal contortions induced by acetic acid in mice.					
32439446	2	39	theme	polysaccharide	245:258	arg1	HASP					270:273	HASP	270:273	HASP	270:273	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	2	39	theme	polysaccharide	245:258	arg1	fraction					260:267	An aqueous soluble polysaccharide fraction	226:267	An aqueous soluble polysaccharide fraction (HASP)	226:274	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	2	40	dep	its	294:296	arg1	leaves					298:303	leaves	298:303	leaves	298:303	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	4	41	theme	mechanical	905:914	arg1	allodynia					916:924	mechanical allodynia	905:924	mechanical allodynia	905:924	Moreover, HASP reduced acetic acid-induced leukocyte infiltration in the peritoneal cavity and showed anti-edematogenic activity, decreasing mechanical allodynia and myeloperoxidase activity in the carrageenan-induced paw edema model.					
32439446	2	42	theme	soluble	237:243	arg1	HASP					270:273	HASP	270:273	HASP	270:273	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	2	42	theme	soluble	237:243	arg1	fraction					260:267	An aqueous soluble polysaccharide fraction	226:267	An aqueous soluble polysaccharide fraction (HASP)	226:274	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	5	43	from	H.	1063:1064	arg1	HASP					1053:1056	the polysaccharide fraction HASP	1025:1056	the polysaccharide fraction HASP from H. albus	1025:1070	These results showed that the polysaccharide fraction HASP from H. albus leaves has interesting antinociceptive and anti-inflammatory activities.					
32439446	1	44	theme	distributed	198:208	arg1	albus					138:142	Handroanthus albus	125:142	Handroanthus albus	125:142	Handroanthus albus, commonly known as yellow ipê, is a native and widely distributed tree in Brazil.					
32439446	1	44	theme	distributed	198:208	arg1	tree					210:213	a native and widely distributed tree	178:213	a native and widely distributed tree in Brazil	178:223	Handroanthus albus, commonly known as yellow ipê, is a native and widely distributed tree in Brazil.					
32439446	2	45	theme	aqueous	229:235	arg1	HASP					270:273	HASP	270:273	HASP	270:273	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	2	45	theme	aqueous	229:235	arg1	fraction					260:267	An aqueous soluble polysaccharide fraction	226:267	An aqueous soluble polysaccharide fraction (HASP)	226:274	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	2	46	theme	linkage	349:355	arg1	analysis					357:364	glycosidic linkage analysis	338:364	glycosidic linkage analysis by methylation	338:379	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	5	47	dep	H.	1063:1064	arg1	albus					1066:1070	H. albus	1063:1070	H. albus	1063:1070	These results showed that the polysaccharide fraction HASP from H. albus leaves has interesting antinociceptive and anti-inflammatory activities.					
32439446	5	48	theme	polysaccharide	1029:1042	arg1	HASP					1053:1056	the polysaccharide fraction HASP	1025:1056	the polysaccharide fraction HASP from H. albus	1025:1070	These results showed that the polysaccharide fraction HASP from H. albus leaves has interesting antinociceptive and anti-inflammatory activities.					
32439446	2	49	theme	glycosidic	338:347	arg1	analysis					357:364	glycosidic linkage analysis	338:364	glycosidic linkage analysis by methylation	338:379	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
32439446	2	50	from	present	526:532	arg1	proportion					547:556	a smaller proportion	537:556	a smaller proportion	537:556	An aqueous soluble polysaccharide fraction (HASP) was obtained from its leaves, and monosaccharide composition, glycosidic linkage analysis by methylation and NMR spectroscopy indicated that HASP is mainly composed of a type II arabinogalactan, and suggested that other polysaccharides could also be present in a smaller proportion.					
34294341	4	0	theme	CM-1	555:558	arg1	activities					541:550	immunoregulatory activities	524:550	immunoregulatory activities	524:550	The anti-breast cancer cells and immunoregulatory activities of CM-1 and CM-2 were investigated.					
34294341	4	0	theme	CM-1	555:558	arg1	cells					514:518	The anti-breast cancer cells	491:518	The anti-breast cancer cells	491:518	The anti-breast cancer cells and immunoregulatory activities of CM-1 and CM-2 were investigated.					
34294341	7	1	theme	inhibiting	1129:1138	arg1	cancer					1140:1145	potential inhibiting cancer and immunostimulatory activities	1119:1178	cancer	1140:1145	Both polysaccharides exhibited potential inhibiting cancer and immunostimulatory activities in vitro, especially CM-1.					
34294341	0	2	with	arabinoxylan	37:48	arg1	activities					129:138	potential antitumor and immunostimulatory activities	87:138	potential antitumor and immunostimulatory activities	87:138	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	5	3	from	arabinose	646:654	arg1	ratio					696:700	a molar ratio	688:700	a molar ratio of 10.78:11.53:1.00:1.70	688:725	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	5	4	theme	10.78:11.53:1.00:1.70	705:725	arg1	ratio					696:700	a molar ratio	688:700	a molar ratio of 10.78:11.53:1.00:1.70	688:725	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	0	5	theme	potential	87:95	arg1	activities					129:138	potential antitumor and immunostimulatory activities	87:138	potential antitumor and immunostimulatory activities	87:138	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	0	6	from	characterization	11:26	arg1	citron					75:80	citron	75:80	citron	75:80	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	5	7	theme	molar	690:694	arg1	ratio					696:700	a molar ratio	688:700	a molar ratio of 10.78:11.53:1.00:1.70	688:725	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	4	8	theme	cancer	507:512	arg1	cells					514:518	The anti-breast cancer cells	491:518	The anti-breast cancer cells	491:518	The anti-breast cancer cells and immunoregulatory activities of CM-1 and CM-2 were investigated.					
34294341	0	9	theme	immunostimulatory	111:127	arg1	activities					129:138	potential antitumor and immunostimulatory activities	87:138	potential antitumor and immunostimulatory activities	87:138	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	4	10	theme	CM-2	564:567	arg1	activities					541:550	immunoregulatory activities	524:550	immunoregulatory activities	524:550	The anti-breast cancer cells and immunoregulatory activities of CM-1 and CM-2 were investigated.					
34294341	4	10	theme	CM-2	564:567	arg1	cells					514:518	The anti-breast cancer cells	491:518	The anti-breast cancer cells	491:518	The anti-breast cancer cells and immunoregulatory activities of CM-1 and CM-2 were investigated.					
34294341	1	11	from	citron	190:195	arg1	polysaccharides					169:183	extract polysaccharides	161:183	extract polysaccharides from citron	161:195	This study aimed to extract polysaccharides from citron and analyze their structures and potential bioactivities.					
34294341	2	12	theme	DEAE-Sepharose	324:337	arg1	Flow					344:347	DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography	324:388	Flow	344:347	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	3	13	theme	linkage	419:425	arg1	data					435:438	Monosaccharide composition, linkage and NMR data	391:438	Monosaccharide composition, linkage and NMR data	391:438	Monosaccharide composition, linkage and NMR data were used to infer their sugar chains composition.					
34294341	4	14	theme	immunoregulatory	524:539	arg1	activities					541:550	immunoregulatory activities	524:550	immunoregulatory activities	524:550	The anti-breast cancer cells and immunoregulatory activities of CM-1 and CM-2 were investigated.					
34294341	7	15	theme	potential	1119:1127	arg1	cancer					1140:1145	potential inhibiting cancer and immunostimulatory activities	1119:1178	cancer	1140:1145	Both polysaccharides exhibited potential inhibiting cancer and immunostimulatory activities in vitro, especially CM-1.					
34294341	5	16	link	-linked	762:768	arg1	skeleton					779:786	(1 → 4)-linked β-d-Xylp skeleton	755:786	(1 → 4)-linked β-d-Xylp skeleton	755:786	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	3	17	theme	Monosaccharide	391:404	arg1	composition					406:416	Monosaccharide composition	391:416	Monosaccharide composition	391:416	Monosaccharide composition, linkage and NMR data were used to infer their sugar chains composition.					
34294341	6	18	theme	β-d-Galp	1044:1051	arg1	units					1053:1057	β-d-Galp units	1044:1057	β-d-Galp units	1044:1057	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	6	19	theme	O-2	1062:1064	arg1	positions					1077:1085	O-2 and/or O-3 positions	1062:1085	positions	1077:1085	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	6	20	theme	O-3	1073:1075	arg1	positions					1077:1085	O-2 and/or O-3 positions	1062:1085	positions	1077:1085	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	3	21	theme	composition	406:416	arg1	data					435:438	Monosaccharide composition, linkage and NMR data	391:438	Monosaccharide composition, linkage and NMR data	391:438	Monosaccharide composition, linkage and NMR data were used to infer their sugar chains composition.					
34294341	5	22	from	position	831:838	arg1	units					818:822	α-l-Araf units	809:822	α-l-Araf units at O-3 position	809:838	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	6	23	from	glucose	902:908	arg1	ratio					935:939	a molar ratio	927:939	a molar ratio of 25.46:1.45:1.00:6.57	927:963	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	2	24	theme	Sephadex	353:360	arg1	chromatography					375:388	DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography	324:388	chromatography	375:388	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	3	25	theme	sugar	465:469	arg1	chains					471:476	their sugar chains	459:476	their sugar chains composition	459:488	Monosaccharide composition, linkage and NMR data were used to infer their sugar chains composition.					
34294341	0	26	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.	0:139	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	5	27	from	glucose	677:683	arg1	ratio					696:700	a molar ratio	688:700	a molar ratio of 10.78:11.53:1.00:1.70	688:725	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	0	28	dep	potential	87:95	arg1	antitumor					97:105	antitumor	97:105	antitumor	97:105	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	3	29	theme	chains	471:476	arg1	composition					478:488	their sugar chains composition	459:488	their sugar chains composition	459:488	Monosaccharide composition, linkage and NMR data were used to infer their sugar chains composition.					
34294341	0	30	theme	novel	31:35	arg1	arabinoxylan					37:48	arabinoxylan	37:48	arabinoxylan	37:48	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	6	31	with	galactoarabinan	970:984	arg1	backbone					1020:1027	(1 → 5)-linked α-l-Araf backbone	996:1027	(1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions	996:1085	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	5	32	dep	monosubstituted	788:802	arg1	skeleton					779:786	(1 → 4)-linked β-d-Xylp skeleton	755:786	(1 → 4)-linked β-d-Xylp skeleton	755:786	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	5	33	theme	-linked	762:768	arg1	skeleton					779:786	(1 → 4)-linked β-d-Xylp skeleton	755:786	(1 → 4)-linked β-d-Xylp skeleton	755:786	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	5	34	from	xylose	657:662	arg1	ratio					696:700	a molar ratio	688:700	a molar ratio of 10.78:11.53:1.00:1.70	688:725	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	3	35	theme	NMR	431:433	arg1	data					435:438	Monosaccharide composition, linkage and NMR data	391:438	Monosaccharide composition, linkage and NMR data	391:438	Monosaccharide composition, linkage and NMR data were used to infer their sugar chains composition.					
34294341	5	36	from	mannose	665:671	arg1	ratio					696:700	a molar ratio	688:700	a molar ratio of 10.78:11.53:1.00:1.70	688:725	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	4	37	theme	anti-breast	495:505	arg1	cells					514:518	The anti-breast cancer cells	491:518	The anti-breast cancer cells	491:518	The anti-breast cancer cells and immunoregulatory activities of CM-1 and CM-2 were investigated.					
34294341	2	38	theme	Fast	339:342	arg1	Flow					344:347	DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography	324:388	Flow	344:347	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	1	39	theme	potential	230:238	arg1	bioactivities					240:252	potential bioactivities	230:252	potential bioactivities	230:252	This study aimed to extract polysaccharides from citron and analyze their structures and potential bioactivities.					
34294341	5	40	theme	α-l-Araf	809:816	arg1	units					818:822	α-l-Araf units	809:822	α-l-Araf units at O-3 position	809:838	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	8	41	theme	citron	1261:1266	arg1	polysaccharides					1268:1282	citron polysaccharides	1261:1282	citron polysaccharides	1261:1282	These results provide a basis for further research on citron polysaccharides.					
34294341	6	42	from	mannose	893:899	arg1	ratio					935:939	a molar ratio	927:939	a molar ratio of 25.46:1.45:1.00:6.57	927:963	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	0	43	theme	arabinoxylan	37:48	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.	0:139	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	2	44	dep	polysaccharides	265:279	arg1	polysaccharides					265:279	Two novel polysaccharides CM-1 and CM-2	255:293	Two novel polysaccharides CM-1 and CM-2	255:293	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	2	44	dep	polysaccharides	265:279	arg1	CM-2					290:293	CM-2	290:293	CM-2	290:293	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	2	44	dep	polysaccharides	265:279	arg1	CM-1					281:284	CM-1	281:284	CM-1	281:284	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	8	45	theme	further	1241:1247	arg1	research					1249:1256	further research	1241:1256	further research on citron polysaccharides	1241:1282	These results provide a basis for further research on citron polysaccharides.					
34294341	6	46	theme	25.46:1.45:1.00:6.57	944:963	arg1	ratio					935:939	a molar ratio	927:939	a molar ratio of 25.46:1.45:1.00:6.57	927:963	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	5	47	theme	O-3	827:829	arg1	position					831:838	O-3 position	827:838	O-3 position	827:838	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	0	48	from	citron	75:80	arg1	galactoarabinan					54:68	galactoarabinan	54:68	galactoarabinan	54:68	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	0	48	from	citron	75:80	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.	0:139	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	0	48	from	citron	75:80	arg1	arabinoxylan					37:48	arabinoxylan	37:48	arabinoxylan	37:48	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	2	49	theme	novel	259:263	arg1	polysaccharides					265:279	Two novel polysaccharides CM-1 and CM-2	255:293	Two novel polysaccharides CM-1 and CM-2	255:293	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	2	49	theme	novel	259:263	arg1	CM-2					290:293	CM-2	290:293	CM-2	290:293	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	2	49	theme	novel	259:263	arg1	CM-1					281:284	CM-1	281:284	CM-1	281:284	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	6	50	dep	backbone	1020:1027	arg1	1 → 5					997:1001	1 → 5	997:1001	1 → 5	997:1001	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	0	51	theme	galactoarabinan	54:68	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.	0:139	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	3	52	used	used	445:448	arg2	data					435:438	Monosaccharide composition, linkage and NMR data	391:438	Monosaccharide composition, linkage and NMR data	391:438	Monosaccharide composition, linkage and NMR data were used to infer their sugar chains composition.					
34294341	7	53	theme	immunostimulatory	1151:1167	arg1	activities					1169:1178	potential inhibiting cancer and immunostimulatory activities	1119:1178	activities	1169:1178	Both polysaccharides exhibited potential inhibiting cancer and immunostimulatory activities in vitro, especially CM-1.					
34294341	5	54	with	monosubstituted	788:802	arg1	units					818:822	α-l-Araf units	809:822	α-l-Araf units at O-3 position	809:838	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	8	55	from	research	1249:1256	arg1	polysaccharides					1268:1282	citron polysaccharides	1261:1282	citron polysaccharides	1261:1282	These results provide a basis for further research on citron polysaccharides.					
34294341	2	56	theme	column	368:373	arg1	chromatography					375:388	DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography	324:388	chromatography	375:388	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	6	57	link	-linked	1003:1009	arg1	backbone					1020:1027	(1 → 5)-linked α-l-Araf backbone	996:1027	(1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions	996:1085	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	2	58	theme	G-100	362:366	arg1	chromatography					375:388	DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography	324:388	chromatography	375:388	Two novel polysaccharides CM-1 and CM-2 were purified from citron by DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography.					
34294341	6	59	theme	α-l-Araf	1011:1018	arg1	backbone					1020:1027	(1 → 5)-linked α-l-Araf backbone	996:1027	(1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions	996:1085	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	5	60	theme	β-d-Xylp	770:777	arg1	skeleton					779:786	(1 → 4)-linked β-d-Xylp skeleton	755:786	(1 → 4)-linked β-d-Xylp skeleton	755:786	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
34294341	0	61	with	galactoarabinan	54:68	arg1	activities					129:138	potential antitumor and immunostimulatory activities	87:138	potential antitumor and immunostimulatory activities	87:138	Structural characterization of novel arabinoxylan and galactoarabinan from citron with potential antitumor and immunostimulatory activities.					
34294341	1	62	theme	extract	161:167	arg1	polysaccharides					169:183	extract polysaccharides	161:183	extract polysaccharides from citron	161:195	This study aimed to extract polysaccharides from citron and analyze their structures and potential bioactivities.					
34294341	6	63	theme	-linked	1003:1009	arg1	backbone					1020:1027	(1 → 5)-linked α-l-Araf backbone	996:1027	(1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions	996:1085	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	6	64	from	galactose	914:922	arg1	ratio					935:939	a molar ratio	927:939	a molar ratio of 25.46:1.45:1.00:6.57	927:963	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	6	65	theme	molar	929:933	arg1	ratio					935:939	a molar ratio	927:939	a molar ratio of 25.46:1.45:1.00:6.57	927:963	While CM-2 (Mw = 22,303 Da), composed of arabinose, mannose, glucose and galactose in a molar ratio of 25.46:1.45:1.00:6.57, was galactoarabinan (GA) with (1 → 5)-linked α-l-Araf backbone substituted by β-d-Galp units at O-2 and/or O-3 positions.					
34294341	5	66	dep	arabinoxylan	732:743	arg1	AX					746:747	AX	746:747	AX	746:747	Results indicated that CM-1 (Mw = 21,520 Da), composed of arabinose, xylose, mannose and glucose in a molar ratio of 10.78:11.53:1.00:1.70, was arabinoxylan (AX) with (1 → 4)-linked β-d-Xylp skeleton monosubstituted with α-l-Araf units at O-3 position.					
33255053	10	0	from	cells	1367:1371	arg1	composites					1384:1393	XAN/PDA composites	1376:1393	XAN/PDA composites	1376:1393	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	1	1	from	polysaccharide	135:148	arg1	industries					161:170	various industries	153:170	various industries	153:170	Xanthan gum (XAN) is a widely used polysaccharide in various industries.					
33255053	3	2	theme	B1	453:454	arg1	cells					463:467	Bacillus thuringiensis B1(2015b) cells	430:467	Bacillus thuringiensis B1(2015b) cells	430:467	The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%.					
33255053	7	3	theme	high	781:784	arg1	stability					786:794	a high stability	779:794	a high stability in a wide range of pH	779:816	XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules.					
33255053	10	4	theme	removal	1334:1340	arg1	acceleration					1247:1258	the significant acceleration	1231:1258	the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal	1231:1340	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	10	5	theme	biodegradation	1268:1281	arg1	acceleration					1247:1258	the significant acceleration	1231:1258	the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal	1231:1340	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	7	6	theme	sorption	829:836	arg1	capacity					838:845	their sorption capacity	823:845	their sorption capacity	823:845	XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules.					
33255053	0	7	theme	immobilised	75:85	arg1	biocatalyst					87:97	a novel immobilised biocatalyst	67:97	a novel immobilised biocatalyst	67:97	Xanthan gum as a carrier for bacterial cell entrapment: Developing a novel immobilised biocatalyst.					
33255053	8	8	theme	rate	918:921	arg1	Analysis					893:900	Analysis	893:900	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl	893:957	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	9	9	with	filter	1177:1182	arg1	microflora					1203:1212	autochthonous microflora	1189:1212	autochthonous microflora	1189:1212	This study also presents the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora.					
33255053	0	10	theme	novel	69:73	arg1	biocatalyst					87:97	a novel immobilised biocatalyst	67:97	a novel immobilised biocatalyst	67:97	Xanthan gum as a carrier for bacterial cell entrapment: Developing a novel immobilised biocatalyst.					
33255053	9	11	theme	naproxen	1104:1111	arg1	biodegradation					1113:1126	naproxen biodegradation	1104:1126	naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora	1104:1212	This study also presents the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora.					
33255053	8	12	theme	survival	909:916	arg1	rate					918:921	the survival rate	905:921	the survival rate during storage at 4 °C in 0.9% NaCl	905:957	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	1	13	theme	Xanthan	100:106	arg1	XAN					113:115	XAN	113:115	XAN	113:115	Xanthan gum (XAN) is a widely used polysaccharide in various industries.					
33255053	1	13	theme	Xanthan	100:106	arg1	gum					108:110	Xanthan gum	100:110	Xanthan gum (XAN)	100:116	Xanthan gum (XAN) is a widely used polysaccharide in various industries.					
33255053	1	13	theme	Xanthan	100:106	arg1	polysaccharide					135:148	a widely used polysaccharide	121:148	a widely used polysaccharide in various industries	121:170	Xanthan gum (XAN) is a widely used polysaccharide in various industries.					
33255053	10	14	theme	XAN/PDA	1376:1382	arg1	composites					1384:1393	XAN/PDA composites	1376:1393	XAN/PDA composites	1376:1393	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	7	15	theme	XAN/PDA	751:757	arg1	composites					759:768	XAN/PDA composites	751:768	XAN/PDA composites	751:768	XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules.					
33255053	1	16	theme	used	130:133	arg1	polysaccharide					135:148	a widely used polysaccharide	121:148	a widely used polysaccharide in various industries	121:170	Xanthan gum (XAN) is a widely used polysaccharide in various industries.					
33255053	1	16	theme	used	130:133	arg1	gum					108:110	Xanthan gum	100:110	Xanthan gum (XAN)	100:116	Xanthan gum (XAN) is a widely used polysaccharide in various industries.					
33255053	8	17	theme	cells	1021:1025	arg1	%					1013:1013	47%	1011:1013	47% of S5 cells entrapped in XAN/PDA	1011:1046	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	8	17	theme	cells	1021:1025	arg1	B1					997:998	B1	997:998	B1(2015b)	997:1005	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	8	17	theme	cells	1021:1025	arg1	%					992:992	98-99%	987:992	98-99% of B1(2015b)	987:1005	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	8	17	theme	cells	1021:1025	arg1	cells					1021:1025	S5 cells	1018:1025	S5 cells entrapped in XAN/PDA	1018:1046	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	8	17	theme	cells	1021:1025	arg1	2015b					1000:1004	2015b	1000:1004	2015b	1000:1004	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	10	18	from	potential	1414:1422	arg1	studies					1442:1448	bioremediation studies	1427:1448	bioremediation studies	1427:1448	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	10	18	from	potential	1414:1422	arg1	applications					1465:1476	industrial applications	1454:1476	industrial applications	1454:1476	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	6	19	theme	composites	681:690	arg1	properties					663:672	good mechanical properties	647:672	good mechanical properties of the composites	647:690	To achieve good mechanical properties of the composites, they were additionally hardened using polydopamine (PDA).					
33255053	5	20	dep	S5	582:583	arg1	which					586:590	which	586:590	which	586:590	S5, which showed a survival rate in the 93-51% range.					
33255053	10	21	from	1 mg/L	1284:1289	arg1	14 days					1294:1300	14 days	1294:1300	14 days	1294:1300	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	4	22	theme	Planococcus	566:576	arg1	sp					578:579	strain Planococcus sp	559:579	strain Planococcus sp	559:579	A negative impact of entrapment was noted for strain Planococcus sp.					
33255053	10	23	dep	biodegradation	1268:1281	arg1	1 mg/L					1284:1289	1 mg/L	1284:1289	1 mg/L in 14 days	1284:1300	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	10	24	theme	2015b	1360:1364	arg1	cells					1367:1371	the entrapped B1(2015b) cells	1343:1371	the entrapped B1(2015b) cells in XAN/PDA composites	1343:1393	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	7	25	from	stability	786:794	arg1	range					806:810	a wide range	799:810	a wide range of pH	799:816	XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules.					
33255053	9	26	theme	biodegradation	1113:1126	arg1	results					1093:1099	the results	1089:1099	the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora	1089:1212	This study also presents the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora.					
33255053	2	27	theme	cells	326:330	arg1	entrapment					302:311	the entrapment	298:311	the entrapment of bacterial cells that are able to biodegrade naproxen	298:367	Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen.					
33255053	0	28	theme	Xanthan	0:6	arg1	gum					8:10	Xanthan gum	0:10	Xanthan gum as a carrier for bacterial cell entrapment:	0:54	Xanthan gum as a carrier for bacterial cell entrapment: Developing a novel immobilised biocatalyst.					
33255053	10	29	theme	drug	1263:1266	arg1	biodegradation					1268:1281	drug biodegradation	1263:1281	drug biodegradation (1 mg/L in 14 days)	1263:1301	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	9	30	theme	trickling	1167:1175	arg1	filter					1177:1182	a trickling filter	1165:1182	a trickling filter with autochthonous microflora	1165:1212	This study also presents the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora.					
33255053	2	31	theme	bacterial	316:324	arg1	cells					326:330	bacterial cells	316:330	bacterial cells that are able to biodegrade naproxen	316:367	Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen.					
33255053	5	32	theme	survival	601:608	arg1	rate					610:613	a survival rate	599:613	a survival rate	599:613	S5, which showed a survival rate in the 93-51% range.					
33255053	10	33	theme	bioremediation	1427:1440	arg1	studies					1442:1448	bioremediation studies	1427:1448	bioremediation studies	1427:1448	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	3	34	theme	survival	491:498	arg1	rate					500:503	a survival rate	489:503	a survival rate of 99%	489:510	The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%.					
33255053	8	35	theme	S5	1018:1019	arg1	cells					1021:1025	S5 cells	1018:1025	S5 cells entrapped in XAN/PDA	1018:1046	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	3	36	theme	thuringiensis	439:451	arg1	cells					463:467	Bacillus thuringiensis B1(2015b) cells	430:467	Bacillus thuringiensis B1(2015b) cells	430:467	The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%.					
33255053	1	37	theme	various	153:159	arg1	industries					161:170	various industries	153:170	various industries	153:170	Xanthan gum (XAN) is a widely used polysaccharide in various industries.					
33255053	4	38	theme	strain	559:564	arg1	sp					578:579	strain Planococcus sp	559:579	strain Planococcus sp	559:579	A negative impact of entrapment was noted for strain Planococcus sp.					
33255053	3	39	theme	developed	374:382	arg1	neutral					418:424	neutral	418:424	neutral	418:424	The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%.					
33255053	3	39	theme	developed	374:382	arg1	procedure					384:392	The developed procedure	370:392	The developed procedure	370:392	The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%.					
33255053	8	40	from	4 °C	941:944	arg1	storage					930:936	storage	930:936	storage at 4 °C in 0.9% NaCl	930:957	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	8	41	theme	%	952:952	arg1	NaCl					954:957	0.9% NaCl	949:957	0.9% NaCl	949:957	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	6	42	theme	mechanical	652:661	arg1	properties					663:672	good mechanical properties	647:672	good mechanical properties of the composites	647:690	To achieve good mechanical properties of the composites, they were additionally hardened using polydopamine (PDA).					
33255053	10	43	theme	B1	1357:1358	arg1	cells					1367:1371	the entrapped B1(2015b) cells	1343:1371	the entrapped B1(2015b) cells in XAN/PDA composites	1343:1393	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	0	44	theme	cell	39:42	arg1	entrapment					44:53	bacterial cell entrapment	29:53	bacterial cell entrapment	29:53	Xanthan gum as a carrier for bacterial cell entrapment: Developing a novel immobilised biocatalyst.					
33255053	5	45	theme	93-51	622:626	arg1	%					627:627	%	627:627	%	627:627	S5, which showed a survival rate in the 93-51% range.					
33255053	10	46	theme	demand	1327:1332	arg1	removal					1334:1340	the chemical oxygen demand removal	1307:1340	the chemical oxygen demand removal	1307:1340	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	2	47	theme	unique	188:193	arg1	properties					195:204	its unique properties	184:204	its unique properties	184:204	Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen.					
33255053	6	48	theme	good	647:650	arg1	properties					663:672	good mechanical properties	647:672	good mechanical properties of the composites	647:690	To achieve good mechanical properties of the composites, they were additionally hardened using polydopamine (PDA).					
33255053	3	49	theme	Bacillus	430:437	arg1	cells					463:467	Bacillus thuringiensis B1(2015b) cells	430:467	Bacillus thuringiensis B1(2015b) cells	430:467	The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%.					
33255053	0	50	theme	bacterial	29:37	arg1	entrapment					44:53	bacterial cell entrapment	29:53	bacterial cell entrapment	29:53	Xanthan gum as a carrier for bacterial cell entrapment: Developing a novel immobilised biocatalyst.					
33255053	8	51	from	storage	930:936	arg1	NaCl					954:957	0.9% NaCl	949:957	0.9% NaCl	949:957	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	8	52	theme	0.9	949:951	arg1	%					952:952	%	952:952	%	952:952	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	3	53	theme	%	510:510	arg1	rate					500:503	a survival rate	489:503	a survival rate of 99%	489:510	The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%.					
33255053	7	54	theme	anionic	874:880	arg1	molecules					882:890	both cationic and anionic molecules	856:890	both cationic and anionic molecules	856:890	XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules.					
33255053	2	55	theme	gum	276:278	arg1	procedure					255:263	the procedure	251:263	the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen	251:367	Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen.					
33255053	2	56	theme	cross-linking	280:292	arg1	gum					276:278	xanthan gum cross-linking	268:292	xanthan gum cross-linking	268:292	Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen.					
33255053	10	57	theme	chemical	1311:1318	arg1	demand					1327:1332	the chemical oxygen demand	1307:1332	the chemical oxygen demand removal	1307:1340	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	7	58	theme	pH	815:816	arg1	range					806:810	a wide range	799:810	a wide range of pH	799:816	XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules.					
33255053	2	59	theme	xanthan	268:274	arg1	gum					276:278	xanthan gum cross-linking	268:292	xanthan gum cross-linking	268:292	Because of its unique properties, in this study, an attempt was made to adopt the procedure of xanthan gum cross-linking for the entrapment of bacterial cells that are able to biodegrade naproxen.					
33255053	7	60	theme	wide	801:804	arg1	range					806:810	a wide range	799:810	a wide range of pH	799:816	XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules.					
33255053	8	61	theme	B1	997:998	arg1	%					1013:1013	47%	1011:1013	47% of S5 cells entrapped in XAN/PDA	1011:1046	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	8	61	theme	B1	997:998	arg1	B1					997:998	B1	997:998	B1(2015b)	997:1005	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	8	61	theme	B1	997:998	arg1	%					992:992	98-99%	987:992	98-99% of B1(2015b)	987:1005	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	8	61	theme	B1	997:998	arg1	cells					1021:1025	S5 cells	1018:1025	S5 cells entrapped in XAN/PDA	1018:1046	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	8	61	theme	B1	997:998	arg1	2015b					1000:1004	2015b	1000:1004	2015b	1000:1004	Analysis of the survival rate during storage at 4 °C in 0.9% NaCl showed that, after 35 days, 98-99% of B1(2015b) and 47% of S5 cells entrapped in XAN/PDA remained alive.					
33255053	9	62	theme	autochthonous	1189:1201	arg1	microflora					1203:1212	autochthonous microflora	1189:1212	autochthonous microflora	1189:1212	This study also presents the results of naproxen biodegradation conducted using XAN/PDA/B1(2015b) in a trickling filter with autochthonous microflora.					
33255053	10	63	theme	significant	1235:1245	arg1	acceleration					1247:1258	the significant acceleration	1231:1258	the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal	1231:1340	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	7	64	theme	cationic	861:868	arg1	molecules					882:890	both cationic and anionic molecules	856:890	both cationic and anionic molecules	856:890	XAN/PDA composites revealed a high stability in a wide range of pH, and their sorption capacity included both cationic and anionic molecules.					
33255053	4	65	theme	negative	515:522	arg1	impact					524:529	A negative impact	513:529	A negative impact of entrapment	513:543	A negative impact of entrapment was noted for strain Planococcus sp.					
33255053	10	66	theme	entrapped	1347:1355	arg1	cells					1367:1371	the entrapped B1(2015b) cells	1343:1371	the entrapped B1(2015b) cells in XAN/PDA composites	1343:1393	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	10	67	theme	industrial	1454:1463	arg1	applications					1465:1476	industrial applications	1454:1476	industrial applications	1454:1476	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	3	68	theme	2015b	456:460	arg1	cells					463:467	Bacillus thuringiensis B1(2015b) cells	430:467	Bacillus thuringiensis B1(2015b) cells	430:467	The developed procedure proved to be completely neutral for Bacillus thuringiensis B1(2015b) cells, which demonstrated a survival rate of 99%.					
33255053	10	69	theme	oxygen	1320:1325	arg1	demand					1327:1332	the chemical oxygen demand	1307:1332	the chemical oxygen demand removal	1307:1340	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	4	70	theme	entrapment	534:543	arg1	impact					524:529	A negative impact	513:529	A negative impact of entrapment	513:543	A negative impact of entrapment was noted for strain Planococcus sp.					
33255053	10	71	theme	promising	1404:1412	arg1	potential					1414:1422	a promising potential	1402:1422	a promising potential in bioremediation studies and industrial applications	1402:1476	Hence, owing to the significant acceleration of drug biodegradation (1 mg/L in 14 days) and the chemical oxygen demand removal, the entrapped B1(2015b) cells in XAN/PDA composites showed a promising potential in bioremediation studies and industrial applications.					
33255053	5	72	theme	%	627:627	arg1	range					629:633	the 93-51% range	618:633	the 93-51% range	618:633	S5, which showed a survival rate in the 93-51% range.					
32039452	5	0	theme	core	789:792	arg1	fucosylation					794:805	plant core fucosylation	783:805	plant core fucosylation	783:805	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	6	1	gly	N-glycoproteins	1098:1112	arg1	N-glycoproteins					1098:1112	two human therapeutic N-glycoproteins	1076:1112	two human therapeutic N-glycoproteins	1076:1112	The engineering was evaluated through coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody.					
32039452	4	2	theme	CHO	739:741	arg1	line					749:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	4	3	theme	α3fucose	636:643	arg1	α3Fuc					646:650	plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes	625:697	α3Fuc	646:650	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	5	4	theme	fucosylation	794:805	arg1	knockin					772:778	directed knockin	763:778	directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT)	763:857	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	1	5	theme	eukaryotic	188:197	arg1	cells					199:203	all eukaryotic cells	184:203	all eukaryotic cells	184:203	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	4	6	theme	core	631:634	arg1	α3Fuc					646:650	plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes	625:697	α3Fuc	646:650	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	4	7	from	α3Fuc	646:650	arg1	line					749:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	8	8	gly	glycoproteins	1498:1510	arg1	glycoproteins					1498:1510	glycoproteins	1498:1510	glycoproteins with plant glycosylation	1498:1535	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	5	9	used	used	758:761	arg2	We					755:756	We	755:756	We	755:756	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	5	10	theme	CHO	973:975	arg1	cells					977:981	CHO cells	973:981	CHO cells with plant N-glycosylation capacities	973:1019	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	7	11	theme	agalactosylated	1235:1249	arg1	G0FX					1268:1271	G0FX	1268:1271	G0FX	1268:1271	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX) was demonstrated for the IgG1 produced in CHO cells.					
32039452	7	11	theme	agalactosylated	1235:1249	arg1	N-glycosylation					1251:1265	the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation	1195:1265	the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX)	1195:1272	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX) was demonstrated for the IgG1 produced in CHO cells.					
32039452	8	12	theme	mammalian	1377:1385	arg1	cells					1387:1391	mammalian cells	1377:1391	mammalian cells	1377:1391	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	5	13	theme	N-glycosylation	994:1008	arg1	capacities					1010:1019	plant N-glycosylation capacities	988:1019	plant N-glycosylation capacities	988:1019	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	5	14	theme	directed	763:770	arg1	knockin					772:778	directed knockin	763:778	directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT)	763:857	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	1	15	located	found	175:179	arg2	N-glycosylation					91:105	Protein N-glycosylation	83:105	Protein N-glycosylation	83:105	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	1	15	located	found	175:179	arg1	cells					199:203	all eukaryotic cells	184:203	all eukaryotic cells	184:203	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	1	15	located	found	175:179	arg2	modification					162:173	an essential and highly conserved posttranslational modification	110:173	an essential and highly conserved posttranslational modification found in all eukaryotic cells	110:203	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	5	16	dep	genes	824:828	arg1	genes					824:828	xylosylation genes	811:828	xylosylation genes (AtFucTA/AtFucTB and AtXylT)	811:857	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	5	16	dep	genes	824:828	arg1	AtXylT					851:856	AtXylT	851:856	AtXylT	851:856	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	5	16	dep	genes	824:828	arg1	AtFucTA/AtFucTB					831:845	AtFucTA/AtFucTB	831:845	AtFucTA/AtFucTB	831:845	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	6	17	theme	erythropoietin	1115:1128	arg1	coexpression					1060:1071	coexpression	1060:1071	coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody	1060:1173	The engineering was evaluated through coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody.					
32039452	1	18	theme	Protein	83:89	arg1	N-glycosylation					91:105	Protein N-glycosylation	83:105	Protein N-glycosylation	83:105	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	1	18	theme	Protein	83:89	arg1	modification					162:173	an essential and highly conserved posttranslational modification	110:173	an essential and highly conserved posttranslational modification found in all eukaryotic cells	110:203	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	6	19	theme	N-glycoproteins	1098:1112	arg1	coexpression					1060:1071	coexpression	1060:1071	coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody	1060:1173	The engineering was evaluated through coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody.					
32039452	5	20	theme	endogenous	884:893	arg1	genes					895:899	endogenous genes	884:899	endogenous genes	884:899	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	7	21	theme	α3Fuc/β2Xyl	1210:1220	arg1	G0FX					1268:1271	G0FX	1268:1271	G0FX	1268:1271	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX) was demonstrated for the IgG1 produced in CHO cells.					
32039452	7	21	theme	α3Fuc/β2Xyl	1210:1220	arg1	N-glycosylation					1251:1265	the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation	1195:1265	the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX)	1195:1272	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX) was demonstrated for the IgG1 produced in CHO cells.					
32039452	8	22	gly	N-glycosylation	1358:1372	arg1	cells					1387:1391	mammalian cells	1377:1391	mammalian cells	1377:1391	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	7	23	theme	bi-antennary	1222:1233	arg1	G0FX					1268:1271	G0FX	1268:1271	G0FX	1268:1271	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX) was demonstrated for the IgG1 produced in CHO cells.					
32039452	7	23	theme	bi-antennary	1222:1233	arg1	N-glycosylation					1251:1265	the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation	1195:1265	the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX)	1195:1272	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX) was demonstrated for the IgG1 produced in CHO cells.					
32039452	0	24	theme	Engineering	0:10	arg1	cells					22:26	Engineering mammalian cells	0:26	Engineering mammalian cells	0:26	Engineering mammalian cells to produce plant-specific N-glycosylation on proteins.					
32039452	5	25	theme	genes	895:899	arg1	knockout					872:879	knockout	872:879	knockout	872:879	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	0	26	theme	mammalian	12:20	arg1	cells					22:26	Engineering mammalian cells	0:26	Engineering mammalian cells	0:26	Engineering mammalian cells to produce plant-specific N-glycosylation on proteins.					
32039452	4	27	used	used	566:569	arg2	we					563:564	we	563:564	we	563:564	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	8	28	from	N-glycosylation	1358:1372	arg1	cells					1387:1391	mammalian cells	1377:1391	mammalian cells	1377:1391	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	8	29	theme	extensive	1409:1417	arg1	engineering					1433:1443	extensive cross-kingdom engineering	1409:1443	extensive cross-kingdom engineering	1409:1443	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	0	30	from	N-glycosylation	54:68	arg1	proteins					73:80	proteins	73:80	proteins	73:80	Engineering mammalian cells to produce plant-specific N-glycosylation on proteins.					
32039452	4	31	theme	Chinese	716:722	arg1	line					749:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	3	32	theme	human	431:435	arg1	use					445:447	human medical use	431:447	human medical use	431:447	These species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines.					
32039452	6	33	theme	antibody	1166:1173	arg1	coexpression					1060:1071	coexpression	1060:1071	coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody	1060:1173	The engineering was evaluated through coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody.					
32039452	4	34	from	epitopes	690:697	arg1	line					749:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	4	35	theme	mammalian	706:714	arg1	line					749:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	3	36	theme	recombinant	402:412	arg1	therapeutics					414:425	recombinant therapeutics	402:425	recombinant therapeutics	402:425	These species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines.					
32039452	8	37	theme	engineered	1454:1463	arg1	cells					1469:1473	engineered CHO cells	1454:1473	engineered CHO cells	1454:1473	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	1	38	theme	essential	113:121	arg1	modification					162:173	an essential and highly conserved posttranslational modification	110:173	an essential and highly conserved posttranslational modification found in all eukaryotic cells	110:203	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	1	38	theme	essential	113:121	arg1	N-glycosylation					91:105	Protein N-glycosylation	83:105	Protein N-glycosylation	83:105	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	3	39	theme	species-specific	503:518	arg1	glycosylation					520:532	species-specific glycosylation	503:532	species-specific glycosylation in design of vaccines	503:554	These species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines.					
32039452	3	40	theme	therapeutics	414:425	arg1	production					388:397	production	388:397	production of recombinant therapeutics	388:425	These species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines.					
32039452	4	41	theme	plant	625:629	arg1	α3Fuc					646:650	plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes	625:697	α3Fuc	646:650	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	3	42	theme	species-specific	309:324	arg1	features					326:333	These species-specific features	303:333	These species-specific features	303:333	These species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines.					
32039452	8	43	theme	cross-kingdom	1419:1431	arg1	engineering					1433:1443	extensive cross-kingdom engineering	1409:1443	extensive cross-kingdom engineering	1409:1443	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	4	44	theme	β2Xyl	667:671	arg1	epitopes					690:697	plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes	625:697	epitopes	690:697	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	0	45	theme	plant-specific	39:52	arg1	N-glycosylation					54:68	plant-specific N-glycosylation	39:68	plant-specific N-glycosylation on proteins	39:80	Engineering mammalian cells to produce plant-specific N-glycosylation on proteins.					
32039452	3	46	theme	vaccines	547:554	arg1	design					537:542	design	537:542	design of vaccines	537:554	These species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines.					
32039452	6	47	theme	G	1158:1158	arg1	antibody					1166:1173	an immunoglobulin G (IgG) antibody	1140:1173	an immunoglobulin G (IgG) antibody	1140:1173	The engineering was evaluated through coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody.					
32039452	3	48	gly	glycosylation	520:532	arg1	design					537:542	design	537:542	design of vaccines	537:554	These species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines.					
32039452	5	49	theme	core	905:908	arg1	fut8					924:927	fut8	924:927	fut8	924:927	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	5	49	theme	core	905:908	arg1	fucosylation					910:921	core fucosylation	905:921	core fucosylation (fut8)	905:928	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	5	50	theme	xylosylation	811:822	arg1	genes					824:828	xylosylation genes	811:828	xylosylation genes (AtFucTA/AtFucTB and AtXylT)	811:857	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	5	50	theme	xylosylation	811:822	arg1	AtXylT					851:856	AtXylT	851:856	AtXylT	851:856	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	5	50	theme	xylosylation	811:822	arg1	AtFucTA/AtFucTB					831:845	AtFucTA/AtFucTB	831:845	AtFucTA/AtFucTB	831:845	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	4	51	theme	reverse	571:577	arg1	engineering					593:603	reverse cross-species engineering	571:603	reverse cross-species engineering	571:603	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	7	52	theme	Full	1176:1179	arg1	conversion					1181:1190	Full conversion	1176:1190	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX)	1176:1272	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX) was demonstrated for the IgG1 produced in CHO cells.					
32039452	5	53	theme	genes	824:828	arg1	knockin					772:778	directed knockin	763:778	directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT)	763:857	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	4	54	theme	N-glycosylation	674:688	arg1	epitopes					690:697	plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes	625:697	epitopes	690:697	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	3	55	theme	medical	437:443	arg1	use					445:447	human medical use	431:447	human medical use	431:447	These species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines.					
32039452	2	56	with	N-glycans	258:266	arg1	features					293:300	distinct structural features	273:300	distinct structural features	273:300	Yeast, plants and mammalian cells, however, produce N-glycans with distinct structural features.					
32039452	5	57	theme	plant	783:787	arg1	fucosylation					794:805	plant core fucosylation	783:805	plant core fucosylation	783:805	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	8	58	theme	CHO	1465:1467	arg1	cells					1469:1473	engineered CHO cells	1454:1473	engineered CHO cells	1454:1473	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	4	59	theme	cell	744:747	arg1	line					749:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	5	60	with	cells	977:981	arg1	capacities					1010:1019	plant N-glycosylation capacities	988:1019	plant N-glycosylation capacities	988:1019	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	6	61	theme	therapeutic	1086:1096	arg1	N-glycoproteins					1098:1112	two human therapeutic N-glycoproteins	1076:1112	two human therapeutic N-glycoproteins	1076:1112	The engineering was evaluated through coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody.					
32039452	8	62	theme	plant	1517:1521	arg1	glycosylation					1523:1535	plant glycosylation	1517:1535	plant glycosylation	1517:1535	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	1	63	theme	conserved	134:142	arg1	modification					162:173	an essential and highly conserved posttranslational modification	110:173	an essential and highly conserved posttranslational modification found in all eukaryotic cells	110:203	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	1	63	theme	conserved	134:142	arg1	N-glycosylation					91:105	Protein N-glycosylation	83:105	Protein N-glycosylation	83:105	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	4	64	theme	cross-species	579:591	arg1	engineering					593:603	reverse cross-species engineering	571:603	reverse cross-species engineering	571:603	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	2	65	theme	mammalian	224:232	arg1	cells					234:238	mammalian cells	224:238	mammalian cells	224:238	Yeast, plants and mammalian cells, however, produce N-glycans with distinct structural features.					
32039452	2	66	theme	structural	282:291	arg1	features					293:300	distinct structural features	273:300	distinct structural features	273:300	Yeast, plants and mammalian cells, however, produce N-glycans with distinct structural features.					
32039452	3	67	from	glycosylation	520:532	arg1	design					537:542	design	537:542	design of vaccines	537:554	These species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines.					
32039452	1	68	theme	posttranslational	144:160	arg1	modification					162:173	an essential and highly conserved posttranslational modification	110:173	an essential and highly conserved posttranslational modification found in all eukaryotic cells	110:203	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	1	68	theme	posttranslational	144:160	arg1	N-glycosylation					91:105	Protein N-glycosylation	83:105	Protein N-glycosylation	83:105	Protein N-glycosylation is an essential and highly conserved posttranslational modification found in all eukaryotic cells.					
32039452	5	69	theme	plant	988:992	arg1	capacities					1010:1019	plant N-glycosylation capacities	988:1019	plant N-glycosylation capacities	988:1019	We used directed knockin of plant core fucosylation and xylosylation genes (AtFucTA/AtFucTB and AtXylT) and targeted knockout of endogenous genes for core fucosylation (fut8) and elongation (B4galt1), for establishing CHO cells with plant N-glycosylation capacities.					
32039452	2	70	theme	distinct	273:280	arg1	features					293:300	distinct structural features	273:300	distinct structural features	273:300	Yeast, plants and mammalian cells, however, produce N-glycans with distinct structural features.					
32039452	8	71	gly	glycosylation	1523:1535	arg1	glycoproteins					1498:1510	glycoproteins	1498:1510	glycoproteins with plant glycosylation	1498:1535	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	8	72	used	used	1482:1485	arg2	cells					1469:1473	engineered CHO cells	1454:1473	engineered CHO cells	1454:1473	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	6	73	theme	immunoglobulin	1143:1156	arg1	IgG					1161:1163	IgG	1161:1163	IgG	1161:1163	The engineering was evaluated through coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody.					
32039452	6	73	theme	immunoglobulin	1143:1156	arg1	G					1158:1158	immunoglobulin G	1143:1158	an immunoglobulin G (IgG) antibody	1140:1173	The engineering was evaluated through coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody.					
32039452	8	74	with	glycoproteins	1498:1510	arg1	glycosylation					1523:1535	plant glycosylation	1517:1535	plant glycosylation	1517:1535	These results demonstrate that N-glycosylation in mammalian cells is amenable for extensive cross-kingdom engineering and that engineered CHO cells may be used to produce glycoproteins with plant glycosylation.					
32039452	0	75	gly	N-glycosylation	54:68	arg1	proteins					73:80	proteins	73:80	proteins	73:80	Engineering mammalian cells to produce plant-specific N-glycosylation on proteins.					
32039452	6	76	theme	human	1080:1084	arg1	N-glycoproteins					1098:1112	two human therapeutic N-glycoproteins	1076:1112	two human therapeutic N-glycoproteins	1076:1112	The engineering was evaluated through coexpression of two human therapeutic N-glycoproteins, erythropoietin (EPO) and an immunoglobulin G (IgG) antibody.					
32039452	4	77	theme	ovary	732:736	arg1	line					749:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	3	78	theme	host	373:376	arg1	cells					378:382	host cells	373:382	host cells	373:382	These species-specific features not only pose challenges in selecting host cells for production of recombinant therapeutics for human medical use but also provide opportunities to explore and utilize species-specific glycosylation in design of vaccines.					
32039452	7	79	theme	plant-type	1199:1208	arg1	G0FX					1268:1271	G0FX	1268:1271	G0FX	1268:1271	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX) was demonstrated for the IgG1 produced in CHO cells.					
32039452	7	79	theme	plant-type	1199:1208	arg1	N-glycosylation					1251:1265	the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation	1195:1265	the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX)	1195:1272	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX) was demonstrated for the IgG1 produced in CHO cells.					
32039452	7	80	theme	CHO	1316:1318	arg1	cells					1320:1324	CHO cells	1316:1324	CHO cells	1316:1324	Full conversion to the plant-type α3Fuc/β2Xyl bi-antennary agalactosylated N-glycosylation (G0FX) was demonstrated for the IgG1 produced in CHO cells.					
32039452	4	81	theme	hamster	724:730	arg1	line					749:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	the mammalian Chinese hamster ovary (CHO) cell line	702:752	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32039452	4	82	theme	β2xylose	657:664	arg1	epitopes					690:697	plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes	625:697	epitopes	690:697	Here, we used reverse cross-species engineering to stably introduce plant core α3fucose (α3Fuc) and β2xylose (β2Xyl) N-glycosylation epitopes in the mammalian Chinese hamster ovary (CHO) cell line.					
32744543	7	0	theme	dietary	1042:1048	arg1	PLP-1					1019:1023	PLP-1	1019:1023	PLP-1	1019:1023	Therefore, PLP-1 could serve as a dietary supplement to ameliorate insulin resistance for diabetic patients.					
32744543	7	0	theme	dietary	1042:1048	arg1	supplement					1050:1059	a dietary supplement	1040:1059	a dietary supplement	1040:1059	Therefore, PLP-1 could serve as a dietary supplement to ameliorate insulin resistance for diabetic patients.					
32744543	0	1	with	homopolysaccharide	33:50	arg1	activity					70:77	hypoglycemic activity	57:77	hypoglycemic activity from the roots of Pueraria lobata	57:111	Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.					
32744543	4	2	theme	7.0 	648:651	arg1	ratio					639:643	a molar ratio	631:643	a molar ratio of 7.0 	631:651	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	1	3	theme	gel	249:251	arg1	chromatography					253:266	DEAE cellulose and Sephadex G-200 gel chromatography	215:266	chromatography	253:266	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	6	4	theme	insulin	983:989	arg1	cells					1001:1005	insulin resistant cells	983:1005	insulin resistant cells	983:1005	The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells.					
32744543	4	5	dep	-α-d-Glcp	593:601	arg1	→4					610:611	→4	610:611	→4	610:611	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	4	5	dep	-α-d-Glcp	593:601	arg1	1→					603:604	1→	603:604	1→	603:604	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	1	6	theme	chromatography	253:266	arg1	purification					268:279	DEAE cellulose and Sephadex G-200 gel chromatography purification	215:279	DEAE cellulose and Sephadex G-200 gel chromatography purification	215:279	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	0	7	theme	lobata	106:111	arg1	roots					88:92	the roots	84:92	the roots of Pueraria lobata	84:111	Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.					
32744543	6	8	theme	glucose	847:853	arg1	concentration					855:867	the glucose concentration	843:867	the glucose concentration	843:867	The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells.					
32744543	0	9	theme	Pueraria	97:104	arg1	lobata					106:111	Pueraria lobata	97:111	Pueraria lobata	97:111	Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.					
32744543	2	10	theme	molecular	294:302	arg1	kDa					329:331	16.2 kDa	324:331	16.2 kDa	324:331	The average molecular weight of PLP-1 was 16.2 kDa.					
32744543	2	10	theme	molecular	294:302	arg1	weight					304:309	The average molecular weight	282:309	The average molecular weight of PLP-1	282:318	The average molecular weight of PLP-1 was 16.2 kDa.					
32744543	5	11	theme	hypoglycemic	673:684	arg1	activity					686:693	the hypoglycemic activity	669:693	the hypoglycemic activity of PLP-1	669:702	Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells.					
32744543	5	12	dep	acid	733:736	arg1	cells					787:791	induced insulin resistant HepG2 cells	755:791	induced insulin resistant HepG2 cells	755:791	Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells.					
32744543	5	13	theme	HepG2	781:785	arg1	cells					787:791	induced insulin resistant HepG2 cells	755:791	induced insulin resistant HepG2 cells	755:791	Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells.					
32744543	2	14	theme	average	286:292	arg1	kDa					329:331	16.2 kDa	324:331	16.2 kDa	324:331	The average molecular weight of PLP-1 was 16.2 kDa.					
32744543	2	14	theme	average	286:292	arg1	weight					304:309	The average molecular weight	282:309	The average molecular weight of PLP-1	282:318	The average molecular weight of PLP-1 was 16.2 kDa.					
32744543	4	15	theme	physical	492:499	arg1	analysis					514:521	extensive physical and chemical analysis	482:521	extensive physical and chemical analysis	482:521	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	5	16	theme	high	742:745	arg1	glucose					747:753	high glucose	742:753	high glucose	742:753	Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells.					
32744543	4	17	theme	extensive	482:490	arg1	analysis					514:521	extensive physical and chemical analysis	482:521	extensive physical and chemical analysis	482:521	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	0	18	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.	0:112	Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.					
32744543	6	19	from	expression	942:951	arg1	cells					1001:1005	insulin resistant cells	983:1005	insulin resistant cells	983:1005	The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells.					
32744543	6	20	theme	resistant	991:999	arg1	cells					1001:1005	insulin resistant cells	983:1005	insulin resistant cells	983:1005	The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells.					
32744543	5	21	theme	induced	755:761	arg1	cells					787:791	induced insulin resistant HepG2 cells	755:791	induced insulin resistant HepG2 cells	755:791	Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells.					
32744543	2	22	theme	PLP-1	314:318	arg1	kDa					329:331	16.2 kDa	324:331	16.2 kDa	324:331	The average molecular weight of PLP-1 was 16.2 kDa.					
32744543	2	22	theme	PLP-1	314:318	arg1	weight					304:309	The average molecular weight	282:309	The average molecular weight of PLP-1	282:318	The average molecular weight of PLP-1 was 16.2 kDa.					
32744543	1	23	theme	Pueraria	196:203	arg1	lobata					205:210	Pueraria lobata	196:210	Pueraria lobata	196:210	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	7	24	theme	diabetic	1098:1105	arg1	patients					1107:1114	diabetic patients	1098:1114	diabetic patients	1098:1114	Therefore, PLP-1 could serve as a dietary supplement to ameliorate insulin resistance for diabetic patients.					
32744543	6	25	theme	AKT	913:915	arg1	expression					890:899	the expression	886:899	the expression of PI3K and AKT	886:915	The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells.					
32744543	1	26	theme	lobata	205:210	arg1	roots					187:191	the roots	183:191	the roots of Pueraria lobata	183:210	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	7	27	theme	insulin	1075:1081	arg1	resistance					1083:1092	insulin resistance	1075:1092	insulin resistance for diabetic patients	1075:1114	Therefore, PLP-1 could serve as a dietary supplement to ameliorate insulin resistance for diabetic patients.					
32744543	4	28	theme	PLP-1	561:565	arg1	backbone					549:556	the backbone	545:556	the backbone of PLP-1	545:565	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	0	29	from	roots	88:92	arg1	activity					70:77	hypoglycemic activity	57:77	hypoglycemic activity from the roots of Pueraria lobata	57:111	Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.					
32744543	6	30	theme	G6Pase	973:978	arg1	expression					942:951	the expression	938:951	the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells	938:1005	The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells.					
32744543	0	31	theme	homopolysaccharide	33:50	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.	0:112	Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.					
32744543	6	32	theme	PI3K	904:907	arg1	expression					890:899	the expression	886:899	the expression of PI3K and AKT	886:915	The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells.					
32744543	5	33	theme	palmitic	724:731	arg1	acid					733:736	palmitic acid	724:736	palmitic acid	724:736	Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells.					
32744543	4	34	dep	composed	578:585	arg1	1→					623:624	1→	623:624	1→	623:624	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	1	35	theme	DEAE	215:218	arg1	cellulose					220:228	DEAE cellulose and Sephadex G-200 gel chromatography	215:266	cellulose	220:228	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	4	36	dep	analysis	514:521	arg1	basis					473:477	basis	473:477	basis	473:477	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	4	36	dep	analysis	514:521	arg1	the					469:471	the	469:471	the	469:471	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	5	37	theme	PLP-1	698:702	arg1	activity					686:693	the hypoglycemic activity	669:693	the hypoglycemic activity of PLP-1	669:702	Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells.					
32744543	1	38	theme	water-soluble	116:128	arg1	homopolysaccharide					138:155	A water-soluble neutral homopolysaccharide	114:155	A water-soluble neutral homopolysaccharide (PLP-1)	114:163	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	1	38	theme	water-soluble	116:128	arg1	PLP-1					158:162	PLP-1	158:162	PLP-1	158:162	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	0	39	theme	hypoglycemic	57:68	arg1	activity					70:77	hypoglycemic activity	57:77	hypoglycemic activity from the roots of Pueraria lobata	57:111	Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.					
32744543	1	40	theme	neutral	130:136	arg1	homopolysaccharide					138:155	A water-soluble neutral homopolysaccharide	114:155	A water-soluble neutral homopolysaccharide (PLP-1)	114:163	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	1	40	theme	neutral	130:136	arg1	PLP-1					158:162	PLP-1	158:162	PLP-1	158:162	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	1	41	theme	cellulose	220:228	arg1	purification					268:279	DEAE cellulose and Sephadex G-200 gel chromatography purification	215:279	DEAE cellulose and Sephadex G-200 gel chromatography purification	215:279	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	4	42	theme	PLP-1	442:446	arg1	structure					429:437	The structure	425:437	The structure of PLP-1	425:446	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	4	43	theme	molar	633:637	arg1	ratio					639:643	a molar ratio	631:643	a molar ratio of 7.0 	631:651	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	6	44	theme	PCK2	963:966	arg1	expression					942:951	the expression	938:951	the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells	938:1005	The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells.					
32744543	3	45	theme	Monosaccharide	334:347	arg1	analysis					361:368	Monosaccharide composition analysis	334:368	Monosaccharide composition analysis	334:368	Monosaccharide composition analysis showed that PLP-1 was composed of glucose as a glucan.					
32744543	4	46	dep	1→	623:624	arg1	 1.0					653:656	 1.0	653:656	 1.0	653:656	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	5	47	theme	resistant	771:779	arg1	cells					787:791	induced insulin resistant HepG2 cells	755:791	induced insulin resistant HepG2 cells	755:791	Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells.					
32744543	1	48	theme	Sephadex	234:241	arg1	chromatography					253:266	DEAE cellulose and Sephadex G-200 gel chromatography	215:266	chromatography	253:266	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32744543	3	49	theme	composition	349:359	arg1	analysis					361:368	Monosaccharide composition analysis	334:368	Monosaccharide composition analysis	334:368	Monosaccharide composition analysis showed that PLP-1 was composed of glucose as a glucan.					
32744543	4	50	theme	chemical	505:512	arg1	analysis					514:521	extensive physical and chemical analysis	482:521	extensive physical and chemical analysis	482:521	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	6	51	theme	FoxO1	956:960	arg1	expression					942:951	the expression	938:951	the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells	938:1005	The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells.					
32744543	4	52	theme	-α-d-Glcp	593:601	arg1	-β-d-Glcp					613:621	→3)-α-d-Glcp(1→ and →4)-β-d-Glcp	590:621	→3)-α-d-Glcp(1→ and →4)-β-d-Glcp	590:621	The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-d-Glcp(1→ and →4)-β-d-Glcp(1→ with a molar ratio of 7.0 : 1.0.					
32744543	1	53	theme	G-200	243:247	arg1	chromatography					253:266	DEAE cellulose and Sephadex G-200 gel chromatography	215:266	chromatography	253:266	A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification.					
32992640	7	0	theme	gut	1365:1367	arg1	health					1369:1374	gut health	1365:1374	gut health (i.e., decreased lipocalin-2)	1365:1404	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	3	1	theme	PM	620:621	arg1	mg/kg					628:632	PM; 200 mg/kg	620:632	PM; 200 mg/kg	620:632	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	1	theme	PM	620:621	arg1	permethrin					608:617	permethrin	608:617	permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total)	608:860	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	2	from	treatment	753:761	arg1	study					711:715	a short-term study	698:715	a short-term study (12 days total)	698:731	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	2	from	treatment	753:761	arg1	total					726:730	12 days total	718:730	12 days total	718:730	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	5	3	theme	global	1084:1089	arg1	scale					1091:1095	a global scale	1082:1095	a global scale	1082:1095	Although PB/PM did not affect species composition on a global scale, it affected specific taxa in both short- and long-term settings.					
32992640	4	4	theme	chemicals	977:985	arg1	effects					962:968	microbiota effects	951:968	microbiota effects of GWI chemicals and acute/delayed LNFPIII administration	951:1026	Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration.					
32992640	4	5	theme	16S	869:871	arg1	sequencing					878:887	Fecal 16S rRNA sequencing	863:887	Fecal 16S rRNA sequencing	863:887	Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration.					
32992640	4	6	theme	acute/delayed	991:1003	arg1	administration					1013:1026	acute/delayed LNFPIII administration	991:1026	acute/delayed LNFPIII administration	991:1026	Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration.					
32992640	3	7	theme	LNFPIII	663:669	arg1	treatment					671:679	concurrent LNFPIII treatment	652:679	concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total)	652:731	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	7	theme	LNFPIII	663:669	arg1	μg/mouse					685:692	35 μg/mouse	682:692	35 μg/mouse	682:692	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	8	8	theme	Multiple	1592:1599	arg1	units					1623:1627	Multiple operational taxonomic units	1592:1627	Multiple operational taxonomic units	1592:1627	Multiple operational taxonomic units correlated with aberrant behavior and lipocalin-2 in PB/PM samples; LNFPIII was modulatory.					
32992640	4	9	theme	microbiota	951:960	arg1	effects					962:968	microbiota effects	951:968	microbiota effects of GWI chemicals and acute/delayed LNFPIII administration	951:1026	Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration.					
32992640	0	10	theme	Mouse	106:110	arg1	Model					112:116	a Mouse Model	104:116	a Mouse Model of Gulf War Illness	104:136	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	8	11	theme	taxonomic	1613:1621	arg1	units					1623:1627	Multiple operational taxonomic units	1592:1627	Multiple operational taxonomic units	1592:1627	Multiple operational taxonomic units correlated with aberrant behavior and lipocalin-2 in PB/PM samples; LNFPIII was modulatory.					
32992640	6	12	theme	prominent	1183:1191	arg1	effects					1203:1209	more prominent long-term effects	1178:1209	more prominent long-term effects	1178:1209	PB/PM elicited more prominent long-term effects, notably, on the abundances of bacteria belonging to Lachnospiraceae and Ruminococcaceae families and the genus Allobaculum.					
32992640	0	13	theme	War	126:128	arg1	Illness					130:136	Gulf War Illness	121:136	Gulf War Illness	121:136	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	3	14	theme	C57BL/6J	533:540	arg1	mice					542:545	Male C57BL/6J mice	528:545	Male C57BL/6J mice	528:545	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	8	15	theme	aberrant	1645:1652	arg1	behavior					1654:1661	aberrant behavior	1645:1661	aberrant behavior	1645:1661	Multiple operational taxonomic units correlated with aberrant behavior and lipocalin-2 in PB/PM samples; LNFPIII was modulatory.					
32992640	3	16	theme	delayed	737:743	arg1	2×/week					764:770	2×/week	764:770	2×/week	764:770	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	16	theme	delayed	737:743	arg1	treatment					753:761	delayed LNFPIII treatment	737:761	delayed LNFPIII treatment (2×/week)	737:771	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	2	17	theme	GWI	517:519	arg1	model					521:525	a GWI model	515:525	a GWI model	515:525	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	2	18	from	benefits	455:462	arg1	health					409:414	gut health	405:414	gut health	405:414	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	2	18	from	benefits	455:462	arg1	microbiota					426:435	fecal microbiota	420:435	fecal microbiota	420:435	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	3	19	theme	short-term	700:709	arg1	study					711:715	a short-term study	698:715	a short-term study (12 days total)	698:731	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	19	theme	short-term	700:709	arg1	total					726:730	12 days total	718:730	12 days total	718:730	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	1	20	from	influence	156:164	arg1	physiology					182:191	host (patho) physiology	169:191	host (patho) physiology	169:191	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	7	21	theme	butyrate	1453:1460	arg1	producers					1462:1470	increased butyrate producers	1443:1470	increased butyrate producers (e.g., Butyricoccus, Ruminococcous)	1443:1506	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	2	22	theme	potential	445:453	arg1	benefits					455:462	the potential benefits	441:462	the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment	441:510	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	4	23	theme	administration	1013:1026	arg1	effects					962:968	microbiota effects	951:968	microbiota effects of GWI chemicals and acute/delayed LNFPIII administration	951:1026	Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration.					
32992640	6	24	theme	bacteria	1242:1249	arg1	abundances					1228:1237	the abundances	1224:1237	the abundances of bacteria belonging to Lachnospiraceae and Ruminococcaceae families and the genus Allobaculum	1224:1333	PB/PM elicited more prominent long-term effects, notably, on the abundances of bacteria belonging to Lachnospiraceae and Ruminococcaceae families and the genus Allobaculum.					
32992640	1	25	theme	chronic	257:263	arg1	Illness					240:246	Gulf War Illness	231:246	Gulf War Illness (GWI)	231:252	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	1	25	theme	chronic	257:263	arg1	disorder					265:272	a chronic disorder	255:272	a chronic disorder featuring dysregulation of the gut-brain-immune axis	255:325	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	3	26	theme	pyridostigmine	565:578	arg1	mg/kg					597:601	PB; 0.7 mg/kg	589:601	PB; 0.7 mg/kg	589:601	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	26	theme	pyridostigmine	565:578	arg1	bromide					580:586	administered pyridostigmine bromide	552:586	administered pyridostigmine bromide (PB; 0.7 mg/kg)	552:602	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	27	theme	days	721:724	arg1	study					711:715	a short-term study	698:715	a short-term study (12 days total)	698:731	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	27	theme	days	721:724	arg1	total					726:730	12 days total	718:730	12 days total	718:730	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	0	28	theme	Gut	75:77	arg1	Microbiota					79:88	Gut Microbiota	75:88	Gut Microbiota	75:88	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	7	29	dep	Ruminococcous	1493:1505	arg1	e.g.					1473:1476	e.g.	1473:1476	e.g.	1473:1476	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	0	30	theme	Gulf	121:124	arg1	Illness					130:136	Gulf War Illness	121:136	Gulf War Illness	121:136	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	7	31	theme	positive	1544:1551	arg1	pressure					1563:1570	a positive selection pressure	1542:1570	a positive selection pressure for these bacteria	1542:1589	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	7	32	theme	GWI	1421:1423	arg1	independent					1406:1416	independent	1406:1416	independent	1406:1416	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	1	33	theme	gut-brain-immune	305:320	arg1	axis					322:325	the gut-brain-immune axis	301:325	the gut-brain-immune axis	301:325	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	4	34	theme	rRNA	873:876	arg1	sequencing					878:887	Fecal 16S rRNA sequencing	863:887	Fecal 16S rRNA sequencing	863:887	Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration.					
32992640	1	35	theme	Gulf	231:234	arg1	Illness					240:246	Gulf War Illness	231:246	Gulf War Illness (GWI)	231:252	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	1	35	theme	Gulf	231:234	arg1	disorder					265:272	a chronic disorder	255:272	a chronic disorder featuring dysregulation of the gut-brain-immune axis	255:325	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	1	35	theme	Gulf	231:234	arg1	GWI					249:251	GWI	249:251	GWI	249:251	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	2	36	theme	GWI-related	380:390	arg1	chemicals					392:400	GWI-related chemicals	380:400	GWI-related chemicals	380:400	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	0	37	theme	Beneficial	14:23	arg1	Effects					25:31	the Beneficial Effects	10:31	the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health	10:99	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	1	38	theme	Illness	240:246	arg1	context					220:226	the context	216:226	the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis	216:325	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	2	39	theme	fecal	420:424	arg1	microbiota					426:435	fecal microbiota	420:435	fecal microbiota	420:435	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	7	40	theme	decreased	1383:1391	arg1	lipocalin-2					1393:1403	decreased lipocalin-2	1383:1403	decreased lipocalin-2	1383:1403	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	4	41	theme	Fecal	863:867	arg1	sequencing					878:887	Fecal 16S rRNA sequencing	863:887	Fecal 16S rRNA sequencing	863:887	Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration.					
32992640	0	42	theme	LNFPIII	64:70	arg1	Effects					25:31	the Beneficial Effects	10:31	the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health	10:99	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	0	43	theme	Immunomodulatory	40:55	arg1	LNFPIII					64:70	the Immunomodulatory Glycan LNFPIII	36:70	the Immunomodulatory Glycan LNFPIII	36:70	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	3	44	theme	exposure	815:822	arg1	days					801:804	10 days	798:804	10 days of PB/PM exposure in a long-term study	798:843	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	7	45	theme	health	1369:1374	arg1	marker					1355:1360	a marker	1353:1360	a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous)	1353:1506	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	3	46	with	days	642:645	arg1	2×/week					764:770	2×/week	764:770	2×/week	764:770	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	46	with	days	642:645	arg1	treatment					671:679	concurrent LNFPIII treatment	652:679	concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total)	652:731	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	46	with	days	642:645	arg1	μg/mouse					685:692	35 μg/mouse	682:692	35 μg/mouse	682:692	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	46	with	days	642:645	arg1	treatment					753:761	delayed LNFPIII treatment	737:761	delayed LNFPIII treatment (2×/week)	737:771	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	2	47	from	effects	369:375	arg1	health					409:414	gut health	405:414	gut health	405:414	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	2	47	from	effects	369:375	arg1	microbiota					426:435	fecal microbiota	420:435	fecal microbiota	420:435	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	9	48	theme	GWI	1754:1756	arg1	effects					1758:1764	significant and lasting GWI effects	1730:1764	significant and lasting GWI effects occurred on specific microbiota and LNFPIII treatment	1730:1818	Overall, significant and lasting GWI effects occurred on specific microbiota and LNFPIII treatment was beneficial.					
32992640	2	49	theme	chemicals	392:400	arg1	benefits					455:462	the potential benefits	441:462	the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment	441:510	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	2	49	theme	chemicals	392:400	arg1	effects					369:375	short- and long-term effects	348:375	short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota	348:435	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	6	50	theme	Allobaculum	1323:1333	arg1	abundances					1228:1237	the abundances	1224:1237	the abundances of bacteria belonging to Lachnospiraceae and Ruminococcaceae families and the genus Allobaculum	1224:1333	PB/PM elicited more prominent long-term effects, notably, on the abundances of bacteria belonging to Lachnospiraceae and Ruminococcaceae families and the genus Allobaculum.					
32992640	3	51	theme	total	855:859	arg1	months					785:790	4 months	783:790	4 months after 10 days of PB/PM exposure in a long-term study (9 months total)	783:860	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	51	theme	total	855:859	arg1	months					848:853	9 months total	846:859	9 months total	846:859	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	9	52	theme	specific	1778:1785	arg1	microbiota					1787:1796	specific microbiota	1778:1796	specific microbiota	1778:1796	Overall, significant and lasting GWI effects occurred on specific microbiota and LNFPIII treatment was beneficial.					
32992640	9	53	theme	LNFPIII	1802:1808	arg1	treatment					1810:1818	LNFPIII treatment	1802:1818	LNFPIII treatment	1802:1818	Overall, significant and lasting GWI effects occurred on specific microbiota and LNFPIII treatment was beneficial.					
32992640	4	54	theme	LNFPIII	1005:1011	arg1	administration					1013:1026	acute/delayed LNFPIII administration	991:1026	acute/delayed LNFPIII administration	991:1026	Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration.					
32992640	1	55	theme	host	169:172	arg1	physiology					182:191	host (patho) physiology	169:191	host (patho) physiology	169:191	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	2	56	theme	treatment	502:510	arg1	benefits					455:462	the potential benefits	441:462	the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment	441:510	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	2	56	theme	treatment	502:510	arg1	effects					369:375	short- and long-term effects	348:375	short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota	348:435	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	4	57	theme	GWI	973:975	arg1	chemicals					977:985	GWI chemicals	973:985	GWI chemicals	973:985	Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration.					
32992640	8	58	from	lipocalin-2	1667:1677	arg1	samples					1688:1694	PB/PM samples	1682:1694	PB/PM samples	1682:1694	Multiple operational taxonomic units correlated with aberrant behavior and lipocalin-2 in PB/PM samples; LNFPIII was modulatory.					
32992640	3	59	theme	concurrent	652:661	arg1	treatment					671:679	concurrent LNFPIII treatment	652:679	concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total)	652:731	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	59	theme	concurrent	652:661	arg1	μg/mouse					685:692	35 μg/mouse	682:692	35 μg/mouse	682:692	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	5	60	from	composition	1067:1077	arg1	scale					1091:1095	a global scale	1082:1095	a global scale	1082:1095	Although PB/PM did not affect species composition on a global scale, it affected specific taxa in both short- and long-term settings.					
32992640	8	61	theme	operational	1601:1611	arg1	units					1623:1627	Multiple operational taxonomic units	1592:1627	Multiple operational taxonomic units	1592:1627	Multiple operational taxonomic units correlated with aberrant behavior and lipocalin-2 in PB/PM samples; LNFPIII was modulatory.					
32992640	1	62	theme	patho	175:179	arg1	physiology					182:191	host (patho) physiology	169:191	host (patho) physiology	169:191	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	2	63	theme	LNFPIII	493:499	arg1	treatment					502:510	Lacto-N-fucopentaose-III (LNFPIII) treatment	467:510	Lacto-N-fucopentaose-III (LNFPIII) treatment	467:510	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	3	64	from	days	801:804	arg1	study					839:843	a long-term study	827:843	a long-term study	827:843	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	8	65	from	behavior	1654:1661	arg1	samples					1688:1694	PB/PM samples	1682:1694	PB/PM samples	1682:1694	Multiple operational taxonomic units correlated with aberrant behavior and lipocalin-2 in PB/PM samples; LNFPIII was modulatory.					
32992640	0	66	theme	Illness	130:136	arg1	Model					112:116	a Mouse Model	104:116	a Mouse Model of Gulf War Illness	104:136	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	3	67	theme	Male	528:531	arg1	mice					542:545	Male C57BL/6J mice	528:545	Male C57BL/6J mice	528:545	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	7	68	theme	producers	1462:1470	arg1	independent					1406:1416	independent	1406:1416	independent	1406:1416	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	5	69	theme	short-	1132:1137	arg1	settings					1153:1160	both short- and long-term settings	1127:1160	both short- and long-term settings	1127:1160	Although PB/PM did not affect species composition on a global scale, it affected specific taxa in both short- and long-term settings.					
32992640	3	70	theme	LNFPIII	745:751	arg1	2×/week					764:770	2×/week	764:770	2×/week	764:770	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	70	theme	LNFPIII	745:751	arg1	treatment					753:761	delayed LNFPIII treatment	737:761	delayed LNFPIII treatment (2×/week)	737:771	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	7	71	theme	increased	1443:1451	arg1	producers					1462:1470	increased butyrate producers	1443:1470	increased butyrate producers (e.g., Butyricoccus, Ruminococcous)	1443:1506	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	5	72	theme	long-term	1143:1151	arg1	settings					1153:1160	both short- and long-term settings	1127:1160	both short- and long-term settings	1127:1160	Although PB/PM did not affect species composition on a global scale, it affected specific taxa in both short- and long-term settings.					
32992640	3	73	theme	administered	552:563	arg1	mg/kg					597:601	PB; 0.7 mg/kg	589:601	PB; 0.7 mg/kg	589:601	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	73	theme	administered	552:563	arg1	bromide					580:586	administered pyridostigmine bromide	552:586	administered pyridostigmine bromide (PB; 0.7 mg/kg)	552:602	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	9	74	theme	significant	1730:1740	arg1	effects					1758:1764	significant and lasting GWI effects	1730:1764	significant and lasting GWI effects occurred on specific microbiota and LNFPIII treatment	1730:1818	Overall, significant and lasting GWI effects occurred on specific microbiota and LNFPIII treatment was beneficial.					
32992640	0	75	from	Effects	25:31	arg1	Health					94:99	Health	94:99	Health	94:99	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	0	75	from	Effects	25:31	arg1	Microbiota					79:88	Gut Microbiota	75:88	Gut Microbiota	75:88	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	5	76	theme	specific	1110:1117	arg1	taxa					1119:1122	specific taxa	1110:1122	specific taxa	1110:1122	Although PB/PM did not affect species composition on a global scale, it affected specific taxa in both short- and long-term settings.					
32992640	8	77	theme	PB/PM	1682:1686	arg1	samples					1688:1694	PB/PM samples	1682:1694	PB/PM samples	1682:1694	Multiple operational taxonomic units correlated with aberrant behavior and lipocalin-2 in PB/PM samples; LNFPIII was modulatory.					
32992640	2	78	theme	Lacto-N-fucopentaose-III	467:490	arg1	treatment					502:510	Lacto-N-fucopentaose-III (LNFPIII) treatment	467:510	Lacto-N-fucopentaose-III (LNFPIII) treatment	467:510	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	7	79	theme	selection	1553:1561	arg1	pressure					1563:1570	a positive selection pressure	1542:1570	a positive selection pressure for these bacteria	1542:1589	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	3	80	from	study	839:843	arg1	days					801:804	10 days	798:804	10 days of PB/PM exposure in a long-term study	798:843	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	6	81	theme	genus	1317:1321	arg1	Allobaculum					1323:1333	the genus Allobaculum	1313:1333	the genus Allobaculum	1313:1333	PB/PM elicited more prominent long-term effects, notably, on the abundances of bacteria belonging to Lachnospiraceae and Ruminococcaceae families and the genus Allobaculum.					
32992640	1	82	theme	axis	322:325	arg1	dysregulation					284:296	dysregulation	284:296	dysregulation of the gut-brain-immune axis	284:325	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	6	83	theme	long-term	1193:1201	arg1	effects					1203:1209	more prominent long-term effects	1178:1209	more prominent long-term effects	1178:1209	PB/PM elicited more prominent long-term effects, notably, on the abundances of bacteria belonging to Lachnospiraceae and Ruminococcaceae families and the genus Allobaculum.					
32992640	3	84	from	treatment	671:679	arg1	study					711:715	a short-term study	698:715	a short-term study (12 days total)	698:731	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	84	from	treatment	671:679	arg1	total					726:730	12 days total	718:730	12 days total	718:730	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	1	85	theme	War	236:238	arg1	Illness					240:246	Gulf War Illness	231:246	Gulf War Illness (GWI)	231:252	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	1	85	theme	War	236:238	arg1	disorder					265:272	a chronic disorder	255:272	a chronic disorder featuring dysregulation of the gut-brain-immune axis	255:325	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	1	85	theme	War	236:238	arg1	GWI					249:251	GWI	249:251	GWI	249:251	The microbiota's influence on host (patho) physiology has gained interest in the context of Gulf War Illness (GWI), a chronic disorder featuring dysregulation of the gut-brain-immune axis.					
32992640	6	86	dep	Lachnospiraceae	1264:1278	arg1	families					1300:1307	families	1300:1307	families	1300:1307	PB/PM elicited more prominent long-term effects, notably, on the abundances of bacteria belonging to Lachnospiraceae and Ruminococcaceae families and the genus Allobaculum.					
32992640	3	87	theme	PB/PM	809:813	arg1	exposure					815:822	PB/PM exposure	809:822	PB/PM exposure in a long-term study	809:843	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	88	from	exposure	815:822	arg1	study					839:843	a long-term study	827:843	a long-term study	827:843	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	2	89	theme	long-term	359:367	arg1	effects					369:375	short- and long-term effects	348:375	short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota	348:435	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	5	90	theme	species	1059:1065	arg1	composition					1067:1077	species composition	1059:1077	species composition on a global scale	1059:1095	Although PB/PM did not affect species composition on a global scale, it affected specific taxa in both short- and long-term settings.					
32992640	7	91	theme	independent	1406:1416	arg1	marker					1355:1360	a marker	1353:1360	a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous)	1353:1506	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	7	92	theme	PB/PM-treated	1511:1523	arg1	mice					1525:1528	PB/PM-treated mice	1511:1528	PB/PM-treated mice	1511:1528	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	3	93	theme	PB	589:590	arg1	mg/kg					597:601	PB; 0.7 mg/kg	589:601	PB; 0.7 mg/kg	589:601	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	3	93	theme	PB	589:590	arg1	bromide					580:586	administered pyridostigmine bromide	552:586	administered pyridostigmine bromide (PB; 0.7 mg/kg)	552:602	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	2	94	theme	short-	348:353	arg1	effects					369:375	short- and long-term effects	348:375	short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota	348:435	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	0	95	theme	Glycan	57:62	arg1	LNFPIII					64:70	the Immunomodulatory Glycan LNFPIII	36:70	the Immunomodulatory Glycan LNFPIII	36:70	Assessing the Beneficial Effects of the Immunomodulatory Glycan LNFPIII on Gut Microbiota and Health in a Mouse Model of Gulf War Illness.					
32992640	3	96	theme	long-term	829:837	arg1	study					839:843	a long-term study	827:843	a long-term study	827:843	Male C57BL/6J mice were administered pyridostigmine bromide (PB; 0.7 mg/kg) and permethrin (PM; 200 mg/kg) for 10 days with concurrent LNFPIII treatment (35 μg/mouse) in a short-term study (12 days total) and delayed LNFPIII treatment (2×/week) beginning 4 months after 10 days of PB/PM exposure in a long-term study (9 months total).					
32992640	2	97	theme	gut	405:407	arg1	health					409:414	gut health	405:414	gut health	405:414	This study examined short- and long-term effects of GWI-related chemicals on gut health and fecal microbiota and the potential benefits of Lacto-N-fucopentaose-III (LNFPIII) treatment in a GWI model.					
32992640	4	98	theme	post-LNFPIII	918:929	arg1	treatment					931:939	post-LNFPIII treatment	918:939	post-LNFPIII treatment	918:939	Fecal 16S rRNA sequencing was performed on all samples post-LNFPIII treatment to assess microbiota effects of GWI chemicals and acute/delayed LNFPIII administration.					
32992640	7	99	dep	lipocalin-2	1393:1403	arg1	i.e.					1377:1380	i.e.	1377:1380	i.e.	1377:1380	LNFPIII improved a marker of gut health (i.e., decreased lipocalin-2) independent of GWI and, importantly, increased butyrate producers (e.g., Butyricoccus, Ruminococcous) in PB/PM-treated mice, indicating a positive selection pressure for these bacteria.					
32992640	9	100	theme	lasting	1746:1752	arg1	effects					1758:1764	significant and lasting GWI effects	1730:1764	significant and lasting GWI effects occurred on specific microbiota and LNFPIII treatment	1730:1818	Overall, significant and lasting GWI effects occurred on specific microbiota and LNFPIII treatment was beneficial.					
32578950	5	0	theme	charged	1107:1113	arg1	surface					1115:1121	a charged surface	1105:1121	a charged surface	1105:1121	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	1	1	from	influence	177:185	arg1	surfaces					231:238	charged surfaces	223:238	charged surfaces	223:238	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	1	1	from	influence	177:185	arg1	behavior					194:201	the behavior	190:201	the behavior of glycoproteins	190:218	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	4	2	attach	presence	831:838	arg2	glycans					843:849	glycans	843:849	glycans	843:849	On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin.					
32578950	4	2	attach	presence	831:838	arg1	avidin					869:874	the acetylated avidin	854:874	the acetylated avidin	854:874	On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin.					
32578950	0	3	theme	Evolution	78:86	arg1	Reaction					88:95	the Catalytic Hydrogen Evolution Reaction	55:95	the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin	55:121	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
32578950	2	4	theme	lysine	618:623	arg1	residues					638:645	lysine and arginine residues	618:645	lysine and arginine residues	618:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	4	5	theme	acetylated	858:867	arg1	avidin					869:874	the acetylated avidin	854:874	the acetylated avidin	854:874	On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin.					
32578950	0	6	theme	Avidin	100:105	arg1	Reaction					88:95	the Catalytic Hydrogen Evolution Reaction	55:95	the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin	55:121	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
32578950	0	7	from	Influence	0:8	arg1	Reaction					88:95	the Catalytic Hydrogen Evolution Reaction	55:95	the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin	55:121	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
32578950	6	8	theme	glycoprotein	1164:1175	arg1	research					1177:1184	glycoprotein research	1164:1184	glycoprotein research	1164:1184	The obtained results can be utilized in glycoprotein research.					
32578950	1	9	theme	nonglycosylated	256:270	arg1	neutravidin					296:306	its nonglycosylated and neutralized version neutravidin	252:306	its nonglycosylated and neutralized version neutravidin	252:306	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	5	10	from	differences	1071:1081	arg1	orientation					1090:1100	the orientation	1086:1100	the orientation at a charged surface	1086:1121	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	5	10	from	differences	1071:1081	arg1	surface					1115:1121	a charged surface	1105:1121	a charged surface	1105:1121	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	2	11	theme	groups	608:613	arg1	amounts					565:571	the different amounts	551:571	the different amounts of catalytically active free amino groups of lysine and arginine residues	551:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	11	theme	groups	608:613	arg1	residues					638:645	lysine and arginine residues	618:645	lysine and arginine residues	618:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	11	theme	groups	608:613	arg1	groups					608:613	catalytically active free amino groups	576:613	catalytically active free amino groups of lysine and arginine residues	576:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	0	12	theme	Neutravidin	111:121	arg1	Reaction					88:95	the Catalytic Hydrogen Evolution Reaction	55:95	the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin	55:121	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
32578950	4	13	theme	acetylated	948:957	arg1	neutravidin					959:969	the acetylated neutravidin	944:969	the acetylated neutravidin	944:969	On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin.					
32578950	1	14	theme	alternating	385:395	arg1	voltammetry					405:415	alternating current voltammetry	385:415	alternating current voltammetry combined with a mercury electrode	385:449	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	5	15	from	surface	1115:1121	arg1	orientation					1090:1100	the orientation	1086:1100	the orientation at a charged surface	1086:1121	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	5	15	from	surface	1115:1121	arg1	differences					1071:1081	differences	1071:1081	differences in the orientation at a charged surface	1071:1121	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	1	16	theme	current	397:403	arg1	voltammetry					405:415	alternating current voltammetry	385:415	alternating current voltammetry combined with a mercury electrode	385:449	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	2	17	theme	residues	638:645	arg1	residues					638:645	lysine and arginine residues	618:645	lysine and arginine residues	618:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	17	theme	residues	638:645	arg1	groups					608:613	catalytically active free amino groups	576:613	catalytically active free amino groups of lysine and arginine residues	576:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	18	theme	CPS	521:523	arg1	responses					525:533	their CPS responses	515:533	their CPS responses	515:533	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	1	19	theme	neutralized	276:286	arg1	neutravidin					296:306	its nonglycosylated and neutralized version neutravidin	252:306	its nonglycosylated and neutralized version neutravidin	252:306	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	1	20	gly	glycoproteins	206:218	arg1	glycoproteins					206:218	glycoproteins	206:218	glycoproteins	206:218	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	5	21	theme	glycans	1030:1036	arg1	composition					1011:1021	composition	1011:1021	composition	1011:1021	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	5	21	theme	glycans	1030:1036	arg1	size					1002:1005	size	1002:1005	size	1002:1005	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	5	21	theme	glycans	1030:1036	arg1	presence					992:999	presence	992:999	presence	992:999	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	1	22	theme	version	288:294	arg1	neutravidin					296:306	its nonglycosylated and neutralized version neutravidin	252:306	its nonglycosylated and neutralized version neutravidin	252:306	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	4	23	theme	chronopotentiometric	902:921	arg1	response					923:930	about 30 % higher chronopotentiometric response	884:930	about 30 % higher chronopotentiometric response	884:930	On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin.					
32578950	1	24	gly	nonglycosylated	256:270	arg1	neutravidin					296:306	its nonglycosylated and neutralized version neutravidin	252:306	its nonglycosylated and neutralized version neutravidin	252:306	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	6	25	theme	obtained	1128:1135	arg1	results					1137:1143	The obtained results	1124:1143	The obtained results	1124:1143	The obtained results can be utilized in glycoprotein research.					
32578950	6	26	used	utilized	1152:1159	arg2	results					1137:1143	The obtained results	1124:1143	The obtained results	1124:1143	The obtained results can be utilized in glycoprotein research.					
32578950	0	27	theme	Protein	13:19	arg1	Influence					0:8	Influence	0:8	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.	0:151	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
32578950	4	28	theme	higher	895:900	arg1	response					923:930	about 30 % higher chronopotentiometric response	884:930	about 30 % higher chronopotentiometric response	884:930	On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin.					
32578950	3	29	theme	proteins	667:674	arg1	Acetylation					648:658	Acetylation	648:658	Acetylation of the proteins	648:674	Acetylation of the proteins resulted in the suppression of their CPS responses by almost four times for avidin and by about 50 % for neutravidin, respectively.					
32578950	0	30	theme	Glycosylation	38:50	arg1	Influence					0:8	Influence	0:8	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.	0:151	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
32578950	2	31	theme	different	555:563	arg1	amounts					565:571	the different amounts	551:571	the different amounts of catalytically active free amino groups of lysine and arginine residues	551:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	31	theme	different	555:563	arg1	residues					638:645	lysine and arginine residues	618:645	lysine and arginine residues	618:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	31	theme	different	555:563	arg1	groups					608:613	catalytically active free amino groups	576:613	catalytically active free amino groups of lysine and arginine residues	576:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	5	32	theme	due	1064:1066	arg1	signal					1057:1062	the CPS signal	1049:1062	the CPS signal due to differences in the orientation at a charged surface	1049:1121	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	4	33	theme	other	815:819	arg1	hand					821:824	the other hand	811:824	the other hand	811:824	On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin.					
32578950	1	34	theme	glycoproteins	206:218	arg1	behavior					194:201	the behavior	190:201	the behavior of glycoproteins	190:218	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	6	35	gly	glycoprotein	1164:1175	arg1	glycoprotein					1164:1175	glycoprotein research	1164:1184	glycoprotein research	1164:1184	The obtained results can be utilized in glycoprotein research.					
32578950	0	36	theme	Electrochemical	127:141	arg1	Analysis					143:150	An Electrochemical Analysis	124:150	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.	0:151	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
32578950	5	37	dep	presence	992:999	arg1	the					988:990	the	988:990	the	988:990	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	2	38	theme	similar	487:493	arg1	size					495:498	neutravidin's and avidin's similar size	460:498	neutravidin's and avidin's similar size	460:498	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	39	theme	amino	602:606	arg1	residues					638:645	lysine and arginine residues	618:645	lysine and arginine residues	618:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	39	theme	amino	602:606	arg1	groups					608:613	catalytically active free amino groups	576:613	catalytically active free amino groups of lysine and arginine residues	576:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	4	40	from	presence	831:838	arg1	avidin					869:874	the acetylated avidin	854:874	the acetylated avidin	854:874	On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin.					
32578950	3	41	theme	CPS	713:715	arg1	responses					717:725	their CPS responses	707:725	their CPS responses	707:725	Acetylation of the proteins resulted in the suppression of their CPS responses by almost four times for avidin and by about 50 % for neutravidin, respectively.					
32578950	1	42	theme	label-free	324:333	arg1	analysis					372:379	label-free chronopotentiometric stripping (CPS) analysis	324:379	label-free chronopotentiometric stripping (CPS) analysis	324:379	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	2	43	theme	free	597:600	arg1	residues					638:645	lysine and arginine residues	618:645	lysine and arginine residues	618:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	43	theme	free	597:600	arg1	groups					608:613	catalytically active free amino groups	576:613	catalytically active free amino groups of lysine and arginine residues	576:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	1	44	theme	mercury	433:439	arg1	electrode					441:449	a mercury electrode	431:449	a mercury electrode	431:449	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	3	45	theme	responses	717:725	arg1	suppression					692:702	the suppression	688:702	the suppression of their CPS responses by almost four times for avidin and by about 50 % for neutravidin	688:791	Acetylation of the proteins resulted in the suppression of their CPS responses by almost four times for avidin and by about 50 % for neutravidin, respectively.					
32578950	1	46	theme	chronopotentiometric	335:354	arg1	CPS					367:369	CPS	367:369	CPS	367:369	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	1	46	theme	chronopotentiometric	335:354	arg1	stripping					356:364	chronopotentiometric stripping	335:364	label-free chronopotentiometric stripping (CPS) analysis	324:379	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	2	47	theme	active	590:595	arg1	residues					638:645	lysine and arginine residues	618:645	lysine and arginine residues	618:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	47	theme	active	590:595	arg1	groups					608:613	catalytically active free amino groups	576:613	catalytically active free amino groups of lysine and arginine residues	576:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	2	48	theme	arginine	629:636	arg1	residues					638:645	lysine and arginine residues	618:645	lysine and arginine residues	618:645	Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues.					
32578950	1	49	theme	charged	223:229	arg1	surfaces					231:238	charged surfaces	223:238	charged surfaces	223:238	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	4	50	theme	glycans	843:849	arg1	presence					831:838	the presence	827:838	the presence of glycans in the acetylated avidin	827:874	On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin.					
32578950	0	51	dep	Protein	13:19	arg1	Modification					21:32	Modification	21:32	Modification	21:32	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
32578950	0	52	theme	Hydrogen	69:76	arg1	Reaction					88:95	the Catalytic Hydrogen Evolution Reaction	55:95	the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin	55:121	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
32578950	5	53	theme	CPS	1053:1055	arg1	signal					1057:1062	the CPS signal	1049:1062	the CPS signal due to differences in the orientation at a charged surface	1049:1121	We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface.					
32578950	0	54	theme	Catalytic	59:67	arg1	Reaction					88:95	the Catalytic Hydrogen Evolution Reaction	55:95	the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin	55:121	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
32578950	1	55	theme	stripping	356:364	arg1	analysis					372:379	label-free chronopotentiometric stripping (CPS) analysis	324:379	label-free chronopotentiometric stripping (CPS) analysis	324:379	To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode.					
32578950	0	56	dep	Influence	0:8	arg1	Analysis					143:150	An Electrochemical Analysis	124:150	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.	0:151	Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis.					
34009670	2	0	theme	weight	461:466	arg1	degree					401:406	their degree	395:406	their degree of substitution, chemical composition, and molecular weight	395:466	Furthermore, their degree of substitution, chemical composition, and molecular weight were evaluated.					
34009670	4	1	theme	highest	677:683	arg1	ability					696:702	the highest scavenging ability	673:702	the highest scavenging ability	673:702	The phosphorylated polysaccharide (SP-P) was demonstrated the highest scavenging ability on hydroxyl radical and growth-promoting activity on Lactobacillus Bulgaricus.					
34009670	1	2	theme	Salep	227:231	arg1	SP					250:251	SP	250:251	SP	250:251	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	2	theme	Salep	227:231	arg1	polysaccharide					234:247	extract Orchis chusua D. Don (Salep) polysaccharide	197:247	extract Orchis chusua D. Don (Salep) polysaccharide (SP)	197:252	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	2	3	theme	molecular	451:459	arg1	weight					461:466	molecular weight	451:466	molecular weight	451:466	Furthermore, their degree of substitution, chemical composition, and molecular weight were evaluated.					
34009670	1	4	mod	modified	270:277	arg3	sulfation					282:290	sulfation	282:290	sulfation	282:290	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	4	mod	modified	270:277	arg1	SP					250:251	SP	250:251	SP	250:251	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	4	mod	modified	270:277	arg3	carboxymethylation					327:344	carboxymethylation	327:344	carboxymethylation	327:344	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	4	mod	modified	270:277	arg3	phosphorylation					306:320	phosphorylation	306:320	phosphorylation	306:320	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	4	mod	modified	270:277	arg3	acetylation					293:303	acetylation	293:303	acetylation	293:303	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	4	mod	modified	270:277	arg1	polysaccharide					234:247	extract Orchis chusua D. Don (Salep) polysaccharide	197:247	extract Orchis chusua D. Don (Salep) polysaccharide (SP)	197:252	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	0	5	from	Effects	0:6	arg1	activities					76:85	biological activities	65:85	biological activities	65:85	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	0	5	from	Effects	0:6	arg1	structure					51:59	structure	51:59	structure	51:59	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	7	6	theme	modified	1148:1155	arg1	polysaccharides					1157:1171	the modified polysaccharides	1144:1171	the modified polysaccharides	1144:1171	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	6	7	theme	stable	1073:1078	arg1	antioxidant					1080:1090	moderately stable antioxidant	1062:1090	moderately stable antioxidant	1062:1090	The acetylated polysaccharide (SP-A) displayed the best proliferation effects on Bifidobacterium adolescentis, whereas the sulfated polysaccharide (SP-S) maintained moderately stable antioxidant and probiotic ability.					
34009670	0	8	theme	polysaccharides	90:104	arg1	activities					76:85	biological activities	65:85	biological activities	65:85	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	0	8	theme	polysaccharides	90:104	arg1	structure					51:59	structure	51:59	structure	51:59	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	0	9	from	structure	51:59	arg1	Don					128:130	Orchis chusua D. Don	111:130	Orchis chusua D. Don	111:130	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	5	10	theme	ABTS	864:867	arg1	scavenging					877:886	ABTS radical scavenging	864:886	ABTS radical scavenging	864:886	The carboxymethylated polysaccharide (SP-C) was exhibited the strongest DPPH and ABTS radical scavenging effects.					
34009670	0	11	theme	chusua	118:123	arg1	Don					128:130	Orchis chusua D. Don	111:130	Orchis chusua D. Don	111:130	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	6	12	theme	acetylated	901:910	arg1	SP-A					928:931	SP-A	928:931	SP-A	928:931	The acetylated polysaccharide (SP-A) displayed the best proliferation effects on Bifidobacterium adolescentis, whereas the sulfated polysaccharide (SP-S) maintained moderately stable antioxidant and probiotic ability.					
34009670	6	12	theme	acetylated	901:910	arg1	polysaccharide					912:925	The acetylated polysaccharide	897:925	The acetylated polysaccharide (SP-A)	897:932	The acetylated polysaccharide (SP-A) displayed the best proliferation effects on Bifidobacterium adolescentis, whereas the sulfated polysaccharide (SP-S) maintained moderately stable antioxidant and probiotic ability.					
34009670	8	13	theme	new	1311:1313	arg1	source					1315:1320	a new source	1309:1320	a new source for the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry	1309:1427	PRACTICAL APPLICATION: This article provides a new source for the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry.					
34009670	1	14	theme	enzyme-assisted	151:165	arg1	method					178:183	an enzyme-assisted extraction method	148:183	an enzyme-assisted extraction method	148:183	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	0	15	theme	Orchis	111:116	arg1	Don					128:130	Orchis chusua D. Don	111:130	Orchis chusua D. Don	111:130	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	4	16	from	activity	745:752	arg1	Bulgaricus					771:780	Lactobacillus Bulgaricus	757:780	Lactobacillus Bulgaricus	757:780	The phosphorylated polysaccharide (SP-P) was demonstrated the highest scavenging ability on hydroxyl radical and growth-promoting activity on Lactobacillus Bulgaricus.					
34009670	8	17	theme	food	1415:1418	arg1	industry					1420:1427	the food industry	1411:1427	the food industry	1411:1427	PRACTICAL APPLICATION: This article provides a new source for the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry.					
34009670	1	18	theme	extraction	167:176	arg1	method					178:183	an enzyme-assisted extraction method	148:183	an enzyme-assisted extraction method	148:183	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	4	19	theme	phosphorylated	619:632	arg1	SP-P					650:653	SP-P	650:653	SP-P	650:653	The phosphorylated polysaccharide (SP-P) was demonstrated the highest scavenging ability on hydroxyl radical and growth-promoting activity on Lactobacillus Bulgaricus.					
34009670	4	19	theme	phosphorylated	619:632	arg1	polysaccharide					634:647	The phosphorylated polysaccharide	615:647	The phosphorylated polysaccharide (SP-P)	615:654	The phosphorylated polysaccharide (SP-P) was demonstrated the highest scavenging ability on hydroxyl radical and growth-promoting activity on Lactobacillus Bulgaricus.					
34009670	2	20	theme	substitution	411:422	arg1	degree					401:406	their degree	395:406	their degree of substitution, chemical composition, and molecular weight	395:466	Furthermore, their degree of substitution, chemical composition, and molecular weight were evaluated.					
34009670	7	21	theme	new	1209:1211	arg1	significance					1193:1204	their potential significance	1177:1204	their potential significance	1177:1204	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	7	21	theme	new	1209:1211	arg1	antioxidants					1213:1224	new antioxidants	1209:1224	new antioxidants	1209:1224	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	6	22	theme	sulfated	1020:1027	arg1	SP-S					1045:1048	SP-S	1045:1048	SP-S	1045:1048	The acetylated polysaccharide (SP-A) displayed the best proliferation effects on Bifidobacterium adolescentis, whereas the sulfated polysaccharide (SP-S) maintained moderately stable antioxidant and probiotic ability.					
34009670	6	22	theme	sulfated	1020:1027	arg1	polysaccharide					1029:1042	the sulfated polysaccharide	1016:1042	the sulfated polysaccharide (SP-S)	1016:1049	The acetylated polysaccharide (SP-A) displayed the best proliferation effects on Bifidobacterium adolescentis, whereas the sulfated polysaccharide (SP-S) maintained moderately stable antioxidant and probiotic ability.					
34009670	3	23	theme	FT-IR	550:554	arg1	spectra					556:562	FT-IR spectra	550:562	FT-IR spectra	550:562	The primary structural features were characterized by UV spectra, FT-IR spectra, Congo-red test, and scanning electron microscope.					
34009670	6	24	theme	probiotic	1096:1104	arg1	ability					1106:1112	probiotic ability	1096:1112	probiotic ability	1096:1112	The acetylated polysaccharide (SP-A) displayed the best proliferation effects on Bifidobacterium adolescentis, whereas the sulfated polysaccharide (SP-S) maintained moderately stable antioxidant and probiotic ability.					
34009670	0	25	theme	chemical	21:28	arg1	modifications					30:42	different chemical modifications	11:42	different chemical modifications	11:42	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	4	26	from	radical	716:722	arg1	Bulgaricus					771:780	Lactobacillus Bulgaricus	757:780	Lactobacillus Bulgaricus	757:780	The phosphorylated polysaccharide (SP-P) was demonstrated the highest scavenging ability on hydroxyl radical and growth-promoting activity on Lactobacillus Bulgaricus.					
34009670	3	27	theme	UV	538:539	arg1	spectra					541:547	UV spectra	538:547	UV spectra	538:547	The primary structural features were characterized by UV spectra, FT-IR spectra, Congo-red test, and scanning electron microscope.					
34009670	3	28	theme	Congo-red	565:573	arg1	test					575:578	Congo-red test	565:578	Congo-red test	565:578	The primary structural features were characterized by UV spectra, FT-IR spectra, Congo-red test, and scanning electron microscope.					
34009670	0	29	theme	different	11:19	arg1	modifications					30:42	different chemical modifications	11:42	different chemical modifications	11:42	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	6	30	theme	Bifidobacterium	978:992	arg1	adolescentis					994:1005	Bifidobacterium adolescentis	978:1005	Bifidobacterium adolescentis	978:1005	The acetylated polysaccharide (SP-A) displayed the best proliferation effects on Bifidobacterium adolescentis, whereas the sulfated polysaccharide (SP-S) maintained moderately stable antioxidant and probiotic ability.					
34009670	0	31	theme	D.	125:126	arg1	Don					128:130	Orchis chusua D. Don	111:130	Orchis chusua D. Don	111:130	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	4	32	theme	growth-promoting	728:743	arg1	activity					745:752	growth-promoting activity	728:752	growth-promoting activity	728:752	The phosphorylated polysaccharide (SP-P) was demonstrated the highest scavenging ability on hydroxyl radical and growth-promoting activity on Lactobacillus Bulgaricus.					
34009670	5	33	theme	strongest	845:853	arg1	DPPH					855:858	DPPH	855:858	DPPH	855:858	The carboxymethylated polysaccharide (SP-C) was exhibited the strongest DPPH and ABTS radical scavenging effects.					
34009670	8	34	dep	APPLICATION	1274:1284	arg1	provides					1300:1307	provides	1300:1307	provides a new source for the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry	1300:1427	PRACTICAL APPLICATION: This article provides a new source for the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry.					
34009670	0	35	from	activities	76:85	arg1	Don					128:130	Orchis chusua D. Don	111:130	Orchis chusua D. Don	111:130	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	0	36	dep	structure	51:59	arg1	the					47:49	the	47:49	the	47:49	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	1	37	theme	extract	197:203	arg1	SP					250:251	SP	250:251	SP	250:251	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	37	theme	extract	197:203	arg1	polysaccharide					234:247	extract Orchis chusua D. Don (Salep) polysaccharide	197:247	extract Orchis chusua D. Don (Salep) polysaccharide (SP)	197:252	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	0	38	theme	modifications	30:42	arg1	Effects					0:6	Effects	0:6	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don	0:130	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	5	39	theme	carboxymethylated	787:803	arg1	polysaccharide					805:818	The carboxymethylated polysaccharide	783:818	The carboxymethylated polysaccharide (SP-C)	783:825	The carboxymethylated polysaccharide (SP-C) was exhibited the strongest DPPH and ABTS radical scavenging effects.					
34009670	5	39	theme	carboxymethylated	787:803	arg1	SP-C					821:824	SP-C	821:824	SP-C	821:824	The carboxymethylated polysaccharide (SP-C) was exhibited the strongest DPPH and ABTS radical scavenging effects.					
34009670	1	40	theme	modified	356:363	arg1	polysaccharides					365:379	modified polysaccharides	356:379	modified polysaccharides	356:379	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	0	41	from	Don	128:130	arg1	activities					76:85	biological activities	65:85	biological activities	65:85	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	0	41	from	Don	128:130	arg1	polysaccharides					90:104	polysaccharides	90:104	polysaccharides from Orchis chusua D. Don	90:130	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	0	41	from	Don	128:130	arg1	structure					51:59	structure	51:59	structure	51:59	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	8	42	theme	new	1375:1377	arg1	antioxidants					1379:1390	new antioxidants	1375:1390	new antioxidants	1375:1390	PRACTICAL APPLICATION: This article provides a new source for the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry.					
34009670	1	43	theme	Orchis	205:210	arg1	SP					250:251	SP	250:251	SP	250:251	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	43	theme	Orchis	205:210	arg1	polysaccharide					234:247	extract Orchis chusua D. Don (Salep) polysaccharide	197:247	extract Orchis chusua D. Don (Salep) polysaccharide (SP)	197:252	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	2	44	theme	composition	434:444	arg1	degree					401:406	their degree	395:406	their degree of substitution, chemical composition, and molecular weight	395:466	Furthermore, their degree of substitution, chemical composition, and molecular weight were evaluated.					
34009670	6	45	theme	proliferation	953:965	arg1	effects					967:973	the best proliferation effects	944:973	the best proliferation effects on Bifidobacterium adolescentis	944:1005	The acetylated polysaccharide (SP-A) displayed the best proliferation effects on Bifidobacterium adolescentis, whereas the sulfated polysaccharide (SP-S) maintained moderately stable antioxidant and probiotic ability.					
34009670	8	46	theme	PRACTICAL	1264:1272	arg1	APPLICATION					1274:1284	PRACTICAL APPLICATION	1264:1284	PRACTICAL APPLICATION: This article provides a new source for the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry.	1264:1428	PRACTICAL APPLICATION: This article provides a new source for the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry.					
34009670	8	47	theme	polysaccharide	1345:1358	arg1	derivatives					1360:1370	polysaccharide derivatives	1345:1370	polysaccharide derivatives as new antioxidants and probiotics for the food industry	1345:1427	PRACTICAL APPLICATION: This article provides a new source for the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry.					
34009670	2	48	theme	chemical	425:432	arg1	composition					434:444	chemical composition	425:444	chemical composition	425:444	Furthermore, their degree of substitution, chemical composition, and molecular weight were evaluated.					
34009670	6	49	theme	best	948:951	arg1	effects					967:973	the best proliferation effects	944:973	the best proliferation effects on Bifidobacterium adolescentis	944:1005	The acetylated polysaccharide (SP-A) displayed the best proliferation effects on Bifidobacterium adolescentis, whereas the sulfated polysaccharide (SP-S) maintained moderately stable antioxidant and probiotic ability.					
34009670	7	50	theme	food	1249:1252	arg1	industry					1254:1261	the food industry	1245:1261	the food industry	1245:1261	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	1	51	used	used	189:192	arg2	method					178:183	an enzyme-assisted extraction method	148:183	an enzyme-assisted extraction method	148:183	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	4	52	theme	scavenging	685:694	arg1	ability					696:702	the highest scavenging ability	673:702	the highest scavenging ability	673:702	The phosphorylated polysaccharide (SP-P) was demonstrated the highest scavenging ability on hydroxyl radical and growth-promoting activity on Lactobacillus Bulgaricus.					
34009670	6	53	from	effects	967:973	arg1	adolescentis					994:1005	Bifidobacterium adolescentis	978:1005	Bifidobacterium adolescentis	978:1005	The acetylated polysaccharide (SP-A) displayed the best proliferation effects on Bifidobacterium adolescentis, whereas the sulfated polysaccharide (SP-S) maintained moderately stable antioxidant and probiotic ability.					
34009670	5	54	dep	DPPH	855:858	arg1	the					841:843	the	841:843	the	841:843	The carboxymethylated polysaccharide (SP-C) was exhibited the strongest DPPH and ABTS radical scavenging effects.					
34009670	5	54	dep	DPPH	855:858	arg1	effects					888:894	effects	888:894	effects	888:894	The carboxymethylated polysaccharide (SP-C) was exhibited the strongest DPPH and ABTS radical scavenging effects.					
34009670	3	55	theme	scanning	585:592	arg1	microscope					603:612	scanning electron microscope	585:612	scanning electron microscope	585:612	The primary structural features were characterized by UV spectra, FT-IR spectra, Congo-red test, and scanning electron microscope.					
34009670	4	56	theme	hydroxyl	707:714	arg1	radical					716:722	hydroxyl radical	707:722	hydroxyl radical	707:722	The phosphorylated polysaccharide (SP-P) was demonstrated the highest scavenging ability on hydroxyl radical and growth-promoting activity on Lactobacillus Bulgaricus.					
34009670	1	57	theme	chusua	212:217	arg1	SP					250:251	SP	250:251	SP	250:251	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	57	theme	chusua	212:217	arg1	polysaccharide					234:247	extract Orchis chusua D. Don (Salep) polysaccharide	197:247	extract Orchis chusua D. Don (Salep) polysaccharide (SP)	197:252	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	3	58	theme	electron	594:601	arg1	microscope					603:612	scanning electron microscope	585:612	scanning electron microscope	585:612	The primary structural features were characterized by UV spectra, FT-IR spectra, Congo-red test, and scanning electron microscope.					
34009670	8	59	theme	derivatives	1360:1370	arg1	development					1330:1340	the development	1326:1340	the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry	1326:1427	PRACTICAL APPLICATION: This article provides a new source for the development of polysaccharide derivatives as new antioxidants and probiotics for the food industry.					
34009670	1	60	theme	D.	219:220	arg1	SP					250:251	SP	250:251	SP	250:251	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	60	theme	D.	219:220	arg1	polysaccharide					234:247	extract Orchis chusua D. Don (Salep) polysaccharide	197:247	extract Orchis chusua D. Don (Salep) polysaccharide (SP)	197:252	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	0	61	theme	biological	65:74	arg1	activities					76:85	biological activities	65:85	biological activities	65:85	Effects of different chemical modifications on the structure and biological activities of polysaccharides from Orchis chusua D. Don.					
34009670	7	62	contain	had	1173:1175	arg1	polysaccharides					1157:1171	the modified polysaccharides	1144:1171	the modified polysaccharides	1144:1171	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	7	62	contain	had	1173:1175	arg2	probiotics					1230:1239	probiotics	1230:1239	probiotics for the food industry	1230:1261	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	7	62	contain	had	1173:1175	arg2	antioxidants					1213:1224	new antioxidants	1209:1224	new antioxidants	1209:1224	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	7	62	contain	had	1173:1175	arg2	significance					1193:1204	their potential significance	1177:1204	their potential significance	1177:1204	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	3	63	theme	primary	488:494	arg1	features					507:514	The primary structural features	484:514	The primary structural features	484:514	The primary structural features were characterized by UV spectra, FT-IR spectra, Congo-red test, and scanning electron microscope.					
34009670	5	64	theme	radical	869:875	arg1	scavenging					877:886	ABTS radical scavenging	864:886	ABTS radical scavenging	864:886	The carboxymethylated polysaccharide (SP-C) was exhibited the strongest DPPH and ABTS radical scavenging effects.					
34009670	1	65	theme	Don	222:224	arg1	SP					250:251	SP	250:251	SP	250:251	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	1	65	theme	Don	222:224	arg1	polysaccharide					234:247	extract Orchis chusua D. Don (Salep) polysaccharide	197:247	extract Orchis chusua D. Don (Salep) polysaccharide (SP)	197:252	In this study, an enzyme-assisted extraction method was used to extract Orchis chusua D. Don (Salep) polysaccharide (SP), which was then modified by sulfation, acetylation, phosphorylation, and carboxymethylation to obtain modified polysaccharides.					
34009670	7	66	theme	potential	1183:1191	arg1	probiotics					1230:1239	probiotics	1230:1239	probiotics for the food industry	1230:1261	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	7	66	theme	potential	1183:1191	arg1	significance					1193:1204	their potential significance	1177:1204	their potential significance	1177:1204	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	7	66	theme	potential	1183:1191	arg1	antioxidants					1213:1224	new antioxidants	1209:1224	new antioxidants	1209:1224	These findings indicate that the modified polysaccharides had their potential significance as new antioxidants and probiotics for the food industry.					
34009670	3	67	theme	structural	496:505	arg1	features					507:514	The primary structural features	484:514	The primary structural features	484:514	The primary structural features were characterized by UV spectra, FT-IR spectra, Congo-red test, and scanning electron microscope.					
32911204	3	0	theme	virulence	508:516	arg1	factor					518:523	an important virulence factor	495:523	an important virulence factor of Pectobacteriaceae	495:544	According to earlier works, lipopolysaccharides are regarded as an important virulence factor of Pectobacteriaceae.					
32911204	3	0	theme	virulence	508:516	arg1	lipopolysaccharides					459:477	lipopolysaccharides	459:477	lipopolysaccharides	459:477	According to earlier works, lipopolysaccharides are regarded as an important virulence factor of Pectobacteriaceae.					
32911204	7	1	theme	1→	1001:1002	arg1	OAc					1007:1009	-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc	973:1009	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.	956:1236	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	5	2	theme	chemical	786:793	arg1	disaccharide					852:863	a linear disaccharide	843:863	a linear disaccharide of the structure shown below	843:892	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	5	2	theme	chemical	786:793	arg1	unit					805:808	the chemical repeating unit	782:808	the chemical repeating unit of the polymer	782:823	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	1	3	theme	pectinolytic	275:286	arg1	bacteria					288:295	the pectinolytic bacteria	271:295	the pectinolytic bacteria isolated from water	271:315	The species Dickeya aquatica was established in 2014 after the genomic characterization of the pectinolytic bacteria isolated from water.					
32911204	5	4	theme	compositional	737:749	arg1	analyses					751:758	compositional analyses	737:758	compositional analyses	737:758	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	7	5	contain	have	1106:1109	arg1	O-polysaccharides					1022:1038	The O-polysaccharides	1018:1038	The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946	1018:1104	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	7	5	contain	have	1106:1109	arg2	structure					1123:1131	a different structure	1111:1131	a different structure	1111:1131	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	1	6	theme	bacteria	288:295	arg1	characterization					251:266	the genomic characterization	239:266	the genomic characterization of the pectinolytic bacteria isolated from water	239:315	The species Dickeya aquatica was established in 2014 after the genomic characterization of the pectinolytic bacteria isolated from water.					
32911204	7	7	theme	OAc	956:958	arg1	↓2					967:968	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%)	956:1016	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.	956:1236	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	7	7	theme	OAc	956:958	arg1	%					1015:1015	~30%	1012:1015	~30%	1012:1015	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	7	8	theme	Dickeya	1215:1221	arg1	strains					1185:1191	several strains	1177:1191	several strains of Dickeya solani and Dickeya dadantii 3937	1177:1235	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	6	9	theme	rhamnose	899:906	arg1	residue					908:914	The rhamnose residue	895:914	The rhamnose residue	895:914	The rhamnose residue was partially acetylated at O-2 or O-3.					
32911204	5	10	theme	linear	845:850	arg1	disaccharide					852:863	a linear disaccharide	843:863	a linear disaccharide of the structure shown below	843:892	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	5	10	theme	linear	845:850	arg1	unit					805:808	the chemical repeating unit	782:808	the chemical repeating unit of the polymer	782:823	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	1	11	theme	species	184:190	arg1	aquatica					200:207	The species Dickeya aquatica	180:207	The species Dickeya aquatica	180:207	The species Dickeya aquatica was established in 2014 after the genomic characterization of the pectinolytic bacteria isolated from water.					
32911204	5	12	theme	NMR	764:766	arg1	spectroscopy					768:779	NMR spectroscopy	764:779	NMR spectroscopy	764:779	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	2	13	from	symptoms	373:380	arg1	fruit					401:405	the fruit	397:405	the fruit of tomato and cucumber	397:428	It was demonstrated that D. aquatica was able to cause symptoms of soft rot on the fruit of tomato and cucumber.					
32911204	4	14	theme	acid	624:627	arg1	hydrolysis					629:638	mild acid hydrolysis	619:638	mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland)	619:722	An O-specific polysaccharide containing d-Fuc and l-Rha was obtained by mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland).					
32911204	1	15	theme	Dickeya	192:198	arg1	aquatica					200:207	The species Dickeya aquatica	180:207	The species Dickeya aquatica	180:207	The species Dickeya aquatica was established in 2014 after the genomic characterization of the pectinolytic bacteria isolated from water.					
32911204	5	16	theme	polymer	817:823	arg1	disaccharide					852:863	a linear disaccharide	843:863	a linear disaccharide of the structure shown below	843:892	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	5	16	theme	polymer	817:823	arg1	unit					805:808	the chemical repeating unit	782:808	the chemical repeating unit of the polymer	782:823	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	0	17	attach	isolated	38:45	arg2	O-polysaccharide					21:36	the O-polysaccharide	17:36	the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154	17:112	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	0	17	attach	isolated	38:45	arg1	bacterium					79:87	pectinolytic gram-negative bacterium Dickeya aquatica IFB0154	52:112	pectinolytic gram-negative bacterium Dickeya aquatica IFB0154	52:112	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	7	18	theme	↑3	1004:1005	arg1	OAc					1007:1009	-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc	973:1009	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.	956:1236	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	5	19	theme	structure	872:880	arg1	disaccharide					852:863	a linear disaccharide	843:863	a linear disaccharide of the structure shown below	843:892	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	5	19	theme	structure	872:880	arg1	unit					805:808	the chemical repeating unit	782:808	the chemical repeating unit of the polymer	782:823	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	2	20	theme	rot	390:392	arg1	symptoms					373:380	symptoms	373:380	symptoms of soft rot on the fruit of tomato and cucumber	373:428	It was demonstrated that D. aquatica was able to cause symptoms of soft rot on the fruit of tomato and cucumber.					
32911204	7	21	dep	dianthicola	1062:1072	arg1	zeae					1094:1097	zeae IPO946	1094:1104	zeae IPO946	1094:1104	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	0	22	dep	bacterium	79:87	arg1	aquatica					97:104	aquatica	97:104	aquatica	97:104	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	7	23	dep	↓2	967:968	arg1	→3					970:971	→3	970:971	→3	970:971	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	7	23	dep	↓2	967:968	arg1	have					1106:1109	have	1106:1109	have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937	1106:1235	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	7	23	dep	↓2	967:968	arg1	OAc					1007:1009	-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc	973:1009	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.	956:1236	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	2	24	theme	cucumber	421:428	arg1	fruit					401:405	the fruit	397:405	the fruit of tomato and cucumber	397:428	It was demonstrated that D. aquatica was able to cause symptoms of soft rot on the fruit of tomato and cucumber.					
32911204	7	25	dep	solani	1204:1209	arg1	3937					1232:1235	dadantii 3937	1223:1235	dadantii 3937	1223:1235	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	4	26	theme	lipopolysaccharide	647:664	arg1	hydrolysis					629:638	mild acid hydrolysis	619:638	mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland)	619:722	An O-specific polysaccharide containing d-Fuc and l-Rha was obtained by mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland).					
32911204	5	27	theme	repeating	795:803	arg1	disaccharide					852:863	a linear disaccharide	843:863	a linear disaccharide of the structure shown below	843:892	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	5	27	theme	repeating	795:803	arg1	unit					805:808	the chemical repeating unit	782:808	the chemical repeating unit of the polymer	782:823	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	0	28	theme	other	157:161	arg1	species					171:177	other Dickeya species	157:177	other Dickeya species	157:177	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	4	29	theme	aquatica	672:679	arg1	lipopolysaccharide					647:664	the lipopolysaccharide	643:664	the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland)	643:722	An O-specific polysaccharide containing d-Fuc and l-Rha was obtained by mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland).					
32911204	4	30	dep	aquatica	672:679	arg1	Dw044					697:701	strain Dw044	690:701	strain Dw044 isolated in Finland	690:721	An O-specific polysaccharide containing d-Fuc and l-Rha was obtained by mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland).					
32911204	4	31	theme	strain	690:695	arg1	Dw044					697:701	strain Dw044	690:701	strain Dw044 isolated in Finland	690:721	An O-specific polysaccharide containing d-Fuc and l-Rha was obtained by mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland).					
32911204	2	32	theme	tomato	410:415	arg1	fruit					401:405	the fruit	397:405	the fruit of tomato and cucumber	397:428	It was demonstrated that D. aquatica was able to cause symptoms of soft rot on the fruit of tomato and cucumber.					
32911204	0	33	theme	O-polysaccharide	21:36	arg1	different					117:125	different	117:125	different	117:125	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	0	33	theme	O-polysaccharide	21:36	arg1	structure					4:12	The structure	0:12	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154	0:112	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	7	34	theme	1 → 4	984:988	arg1	OAc					1007:1009	-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc	973:1009	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.	956:1236	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	7	35	theme	identical	1134:1142	arg1	structure					1123:1131	a different structure	1111:1131	a different structure	1111:1131	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	0	36	theme	pectinolytic	52:63	arg1	bacterium					79:87	pectinolytic gram-negative bacterium Dickeya aquatica IFB0154	52:112	pectinolytic gram-negative bacterium Dickeya aquatica IFB0154	52:112	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	0	37	theme	species	171:177	arg1	O-polysaccharides					136:152	the O-polysaccharides	132:152	the O-polysaccharides of other Dickeya species	132:177	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	7	38	theme	several	1177:1183	arg1	strains					1185:1191	several strains	1177:1191	several strains of Dickeya solani and Dickeya dadantii 3937	1177:1235	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	7	39	theme	dadantii	1223:1230	arg1	3937					1232:1235	dadantii 3937	1223:1235	dadantii 3937	1223:1235	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	0	40	theme	Dickeya	163:169	arg1	species					171:177	other Dickeya species	157:177	other Dickeya species	157:177	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	4	41	theme	mild	619:622	arg1	hydrolysis					629:638	mild acid hydrolysis	619:638	mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland)	619:722	An O-specific polysaccharide containing d-Fuc and l-Rha was obtained by mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland).					
32911204	7	42	theme	-α-d-Fucp-	973:982	arg1	OAc					1007:1009	-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc	973:1009	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.	956:1236	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	3	43	theme	important	498:506	arg1	factor					518:523	an important virulence factor	495:523	an important virulence factor of Pectobacteriaceae	495:544	According to earlier works, lipopolysaccharides are regarded as an important virulence factor of Pectobacteriaceae.					
32911204	3	43	theme	important	498:506	arg1	lipopolysaccharides					459:477	lipopolysaccharides	459:477	lipopolysaccharides	459:477	According to earlier works, lipopolysaccharides are regarded as an important virulence factor of Pectobacteriaceae.					
32911204	7	44	attach	isolated	1040:1047	arg1	dianthicola					1062:1072	dianthicola	1062:1072	dianthicola	1062:1072	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	7	44	attach	isolated	1040:1047	arg1	Dickeya					1086:1092	Dickeya	1086:1092	Dickeya	1086:1092	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	7	44	attach	isolated	1040:1047	arg2	O-polysaccharides					1022:1038	The O-polysaccharides	1018:1038	The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946	1018:1104	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	4	45	theme	O-specific	550:559	arg1	polysaccharide					561:574	An O-specific polysaccharide	547:574	An O-specific polysaccharide containing d-Fuc and l-Rha	547:601	An O-specific polysaccharide containing d-Fuc and l-Rha was obtained by mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland).					
32911204	7	46	theme	-α-l-Rhap-	990:999	arg1	OAc					1007:1009	-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc	973:1009	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.	956:1236	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	2	47	dep	D.	343:344	arg1	aquatica					346:353	D. aquatica	343:353	D. aquatica	343:353	It was demonstrated that D. aquatica was able to cause symptoms of soft rot on the fruit of tomato and cucumber.					
32911204	4	48	contain	containing	576:585	arg2	l-Rha					597:601	l-Rha	597:601	l-Rha	597:601	An O-specific polysaccharide containing d-Fuc and l-Rha was obtained by mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland).					
32911204	4	48	contain	containing	576:585	arg2	d-Fuc					587:591	d-Fuc	587:591	d-Fuc	587:591	An O-specific polysaccharide containing d-Fuc and l-Rha was obtained by mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland).					
32911204	4	48	contain	containing	576:585	arg1	polysaccharide					561:574	An O-specific polysaccharide	547:574	An O-specific polysaccharide containing d-Fuc and l-Rha	547:601	An O-specific polysaccharide containing d-Fuc and l-Rha was obtained by mild acid hydrolysis of the lipopolysaccharide of D. aquatica IFB0154 (strain Dw044 isolated in Finland).					
32911204	5	49	dep	analyses	751:758	arg1	means					728:732	means	728:732	means	728:732	By means of compositional analyses and NMR spectroscopy, the chemical repeating unit of the polymer was identified as a linear disaccharide of the structure shown below.					
32911204	7	50	theme	different	1113:1121	arg1	structure					1123:1131	a different structure	1111:1131	a different structure	1111:1131	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	7	51	theme	solani	1204:1209	arg1	strains					1185:1191	several strains	1177:1191	several strains of Dickeya solani and Dickeya dadantii 3937	1177:1235	OAc (~40%) ↓2 →3)-α-d-Fucp-(1 → 4)-α-l-Rhap-(1→ ↑3 OAc (~30%) The O-polysaccharides isolated from Dickeya dianthicola IFB0485 and Dickeya zeae IPO946 have a different structure, identical to that previously described for several strains of Dickeya solani and Dickeya dadantii 3937.					
32911204	1	52	theme	genomic	243:249	arg1	characterization					251:266	the genomic characterization	239:266	the genomic characterization of the pectinolytic bacteria isolated from water	239:315	The species Dickeya aquatica was established in 2014 after the genomic characterization of the pectinolytic bacteria isolated from water.					
32911204	1	53	attach	isolated	297:304	arg2	bacteria					288:295	the pectinolytic bacteria	271:295	the pectinolytic bacteria isolated from water	271:315	The species Dickeya aquatica was established in 2014 after the genomic characterization of the pectinolytic bacteria isolated from water.					
32911204	1	53	attach	isolated	297:304	arg1	water					311:315	water	311:315	water	311:315	The species Dickeya aquatica was established in 2014 after the genomic characterization of the pectinolytic bacteria isolated from water.					
32911204	2	54	theme	soft	385:388	arg1	rot					390:392	soft rot	385:392	soft rot	385:392	It was demonstrated that D. aquatica was able to cause symptoms of soft rot on the fruit of tomato and cucumber.					
32911204	3	55	theme	earlier	444:450	arg1	works					452:456	earlier works	444:456	earlier works	444:456	According to earlier works, lipopolysaccharides are regarded as an important virulence factor of Pectobacteriaceae.					
32911204	0	56	from	O-polysaccharides	136:152	arg1	different					117:125	different	117:125	different	117:125	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	0	56	from	O-polysaccharides	136:152	arg1	structure					4:12	The structure	0:12	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154	0:112	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	0	57	theme	gram-negative	65:77	arg1	bacterium					79:87	pectinolytic gram-negative bacterium Dickeya aquatica IFB0154	52:112	pectinolytic gram-negative bacterium Dickeya aquatica IFB0154	52:112	The structure of the O-polysaccharide isolated from pectinolytic gram-negative bacterium Dickeya aquatica IFB0154 is different from the O-polysaccharides of other Dickeya species.					
32911204	3	58	theme	Pectobacteriaceae	528:544	arg1	factor					518:523	an important virulence factor	495:523	an important virulence factor of Pectobacteriaceae	495:544	According to earlier works, lipopolysaccharides are regarded as an important virulence factor of Pectobacteriaceae.					
32911204	3	58	theme	Pectobacteriaceae	528:544	arg1	lipopolysaccharides					459:477	lipopolysaccharides	459:477	lipopolysaccharides	459:477	According to earlier works, lipopolysaccharides are regarded as an important virulence factor of Pectobacteriaceae.					
32070744	0	0	theme	enzyme	78:83	arg1	α-glucosidase					86:98	α-glucosidase	86:98	α-glucosidase	86:98	Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.					
32070744	0	0	theme	enzyme	78:83	arg1	Fucoidan					0:7	Fucoidan	0:7	Fucoidan from Ecklonia maxima	0:28	Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.					
32070744	0	0	theme	enzyme	78:83	arg1	inhibitor					44:52	a powerful inhibitor	33:52	a powerful inhibitor of the diabetes-related enzyme	33:83	Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.					
32070744	9	1	theme	present	1239:1245	arg1	study					1247:1251	Our present study	1235:1251	Our present study	1235:1251	Our present study demonstrated that fucoidan from E. maxima is a more powerful inhibitor compared to some standard anti-diabetic compounds and thus shows great potential for managing type 2 diabetes.					
32070744	9	2	theme	standard	1341:1348	arg1	compounds					1364:1372	some standard anti-diabetic compounds	1336:1372	some standard anti-diabetic compounds	1336:1372	Our present study demonstrated that fucoidan from E. maxima is a more powerful inhibitor compared to some standard anti-diabetic compounds and thus shows great potential for managing type 2 diabetes.					
32070744	6	3	dep	studies	725:731	arg1	XRD					749:751	XRD	749:751	XRD	749:751	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	6	3	dep	studies	725:731	arg1	NMR					741:743	NMR	741:743	NMR	741:743	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	6	3	dep	studies	725:731	arg1	FT-IR					734:738	FT-IR	734:738	FT-IR	734:738	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	6	3	dep	studies	725:731	arg1	studies					725:731	Structural studies	714:731	Structural studies (FT-IR, NMR and XRD)	714:752	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	0	4	theme	diabetes-related	61:76	arg1	enzyme					78:83	the diabetes-related enzyme	57:83	the diabetes-related enzyme	57:83	Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.					
32070744	9	5	theme	type	1418:1421	arg1	diabetes					1425:1432	type 2 diabetes	1418:1432	type 2 diabetes	1418:1432	Our present study demonstrated that fucoidan from E. maxima is a more powerful inhibitor compared to some standard anti-diabetic compounds and thus shows great potential for managing type 2 diabetes.					
32070744	5	6	theme	extracted	633:641	arg1	fucoidan					653:660	The water extracted E. maxima fucoidan	623:660	The water extracted E. maxima fucoidan	623:660	The water extracted E. maxima fucoidan had a low molecular weight of approximately 10 kDa.					
32070744	1	7	theme	Ecklonia	101:108	arg1	maxima					110:115	Ecklonia maxima	101:115	Ecklonia maxima	101:115	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	1	7	theme	Ecklonia	101:108	arg1	source					167:172	a potential source	155:172	a potential source of beneficial bioactive compounds	155:206	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	1	7	theme	Ecklonia	101:108	arg1	seaweed					143:149	an endemic South African seaweed	118:149	an endemic South African seaweed	118:149	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	5	8	theme	10 kDa	706:711	arg1	weight					682:687	a low molecular weight	666:687	a low molecular weight of approximately 10 kDa	666:711	The water extracted E. maxima fucoidan had a low molecular weight of approximately 10 kDa.					
32070744	5	9	theme	E.	643:644	arg1	fucoidan					653:660	The water extracted E. maxima fucoidan	623:660	The water extracted E. maxima fucoidan	623:660	The water extracted E. maxima fucoidan had a low molecular weight of approximately 10 kDa.					
32070744	7	10	theme	digestive	903:911	arg1	enzymes					913:919	starch digestive enzymes	896:919	starch digestive enzymes; α-amylase and α-glucosidase	896:948	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	7	10	theme	digestive	903:911	arg1	α-glucosidase					936:948	α-glucosidase	936:948	α-glucosidase	936:948	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	7	10	theme	digestive	903:911	arg1	α-amylase					922:930	α-amylase	922:930	α-amylase	922:930	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	9	11	theme	great	1389:1393	arg1	potential					1395:1403	great potential	1389:1403	great potential for managing type 2 diabetes	1389:1432	Our present study demonstrated that fucoidan from E. maxima is a more powerful inhibitor compared to some standard anti-diabetic compounds and thus shows great potential for managing type 2 diabetes.					
32070744	8	12	theme	IC50	1118:1121	arg1	range					1123:1127	an IC50 range	1115:1127	an IC50 range	1115:1127	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	8	12	theme	IC50	1118:1121	arg1	lower					1175:1179	lower	1175:1179	lower	1175:1179	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	4	13	theme	6.01 ± 0.53	600:610	arg1	%					611:611	%	611:611	%	611:611	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	3	14	theme	hot	397:399	arg1	method					418:423	the hot water extraction method	393:423	the hot water extraction method	393:423	In this study, fucoidan was extracted from E. maxima by the hot water extraction method and then characterised by colorimetric assays for sugar composition.					
32070744	3	15	theme	extraction	407:416	arg1	method					418:423	the hot water extraction method	393:423	the hot water extraction method	393:423	In this study, fucoidan was extracted from E. maxima by the hot water extraction method and then characterised by colorimetric assays for sugar composition.					
32070744	6	16	from	fucoidans	844:852	arg1	literature					857:866	literature	857:866	literature	857:866	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	6	17	theme	studied	836:842	arg1	fucoidans					844:852	previously studied fucoidans	825:852	previously studied fucoidans in literature	825:866	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	2	18	theme	sulphated	244:252	arg1	fucoidan					232:239	fucoidan	232:239	fucoidan	232:239	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	2	18	theme	sulphated	244:252	arg1	polysaccharide					254:267	a sulphated polysaccharide	242:267	a sulphated polysaccharide	242:267	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	5	19	dep	extracted	633:641	arg1	water					627:631	water	627:631	water	627:631	The water extracted E. maxima fucoidan had a low molecular weight of approximately 10 kDa.					
32070744	9	20	theme	E.	1285:1286	arg1	maxima					1288:1293	E. maxima	1285:1293	E. maxima	1285:1293	Our present study demonstrated that fucoidan from E. maxima is a more powerful inhibitor compared to some standard anti-diabetic compounds and thus shows great potential for managing type 2 diabetes.					
32070744	9	21	from	maxima	1288:1293	arg1	fucoidan					1271:1278	fucoidan	1271:1278	fucoidan from E. maxima	1271:1293	Our present study demonstrated that fucoidan from E. maxima is a more powerful inhibitor compared to some standard anti-diabetic compounds and thus shows great potential for managing type 2 diabetes.					
32070744	9	21	from	maxima	1288:1293	arg1	inhibitor					1314:1322	a more powerful inhibitor	1298:1322	a more powerful inhibitor compared to some standard anti-diabetic compounds	1298:1372	Our present study demonstrated that fucoidan from E. maxima is a more powerful inhibitor compared to some standard anti-diabetic compounds and thus shows great potential for managing type 2 diabetes.					
32070744	7	22	theme	starch	896:901	arg1	enzymes					913:919	starch digestive enzymes	896:919	starch digestive enzymes; α-amylase and α-glucosidase	896:948	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	7	22	theme	starch	896:901	arg1	α-glucosidase					936:948	α-glucosidase	936:948	α-glucosidase	936:948	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	7	22	theme	starch	896:901	arg1	α-amylase					922:930	α-amylase	922:930	α-amylase	922:930	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	2	23	theme	bioactivities	289:301	arg1	activity					327:334	anti-diabetic activity	313:334	anti-diabetic activity	313:334	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	2	23	theme	bioactivities	289:301	arg1	range					280:284	a wide range	273:284	a wide range of bioactivities including anti-diabetic activity	273:334	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	8	24	theme	E.	1034:1035	arg1	maxima					1037:1042	E. maxima	1034:1042	E. maxima	1034:1042	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	1	25	theme	potential	157:165	arg1	maxima					110:115	Ecklonia maxima	101:115	Ecklonia maxima	101:115	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	1	25	theme	potential	157:165	arg1	source					167:172	a potential source	155:172	a potential source of beneficial bioactive compounds	155:206	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	4	26	theme	%	536:536	arg1	fucoidan					538:545	6.89% fucoidan	532:545	6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate	532:620	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	3	27	theme	water	401:405	arg1	method					418:423	the hot water extraction method	393:423	the hot water extraction method	393:423	In this study, fucoidan was extracted from E. maxima by the hot water extraction method and then characterised by colorimetric assays for sugar composition.					
32070744	4	28	theme	4.45 ± 0.25	574:584	arg1	%					585:585	%	585:585	%	585:585	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	4	29	theme	6.89	532:535	arg1	%					536:536	%	536:536	%	536:536	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	5	30	theme	low	668:670	arg1	weight					682:687	a low molecular weight	666:687	a low molecular weight of approximately 10 kDa	666:711	The water extracted E. maxima fucoidan had a low molecular weight of approximately 10 kDa.					
32070744	7	31	theme	enzymes	913:919	arg1	activities					882:891	the activities	878:891	the activities	878:891	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	4	32	theme	%	611:611	arg1	sulphate					613:620	6.01 ± 0.53% sulphate	600:620	6.01 ± 0.53% sulphate	600:620	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	0	33	theme	Ecklonia	14:21	arg1	maxima					23:28	Ecklonia maxima	14:28	Ecklonia maxima	14:28	Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.					
32070744	2	34	contain	has	269:271	arg1	fucoidan					232:239	fucoidan	232:239	fucoidan	232:239	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	2	34	contain	has	269:271	arg2	range					280:284	a wide range	273:284	a wide range of bioactivities including anti-diabetic activity	273:334	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	2	34	contain	has	269:271	arg1	polysaccharide					254:267	a sulphated polysaccharide	242:267	a sulphated polysaccharide	242:267	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	2	34	contain	has	269:271	arg2	activity					327:334	anti-diabetic activity	313:334	anti-diabetic activity	313:334	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	5	35	theme	molecular	672:680	arg1	weight					682:687	a low molecular weight	666:687	a low molecular weight of approximately 10 kDa	666:711	The water extracted E. maxima fucoidan had a low molecular weight of approximately 10 kDa.					
32070744	2	36	theme	wide	275:278	arg1	activity					327:334	anti-diabetic activity	313:334	anti-diabetic activity	313:334	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	2	36	theme	wide	275:278	arg1	range					280:284	a wide range	273:284	a wide range of bioactivities including anti-diabetic activity	273:334	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	1	37	theme	beneficial	177:186	arg1	compounds					198:206	beneficial bioactive compounds	177:206	beneficial bioactive compounds	177:206	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	7	38	theme	fucoidan	1002:1009	arg1	extract					1011:1017	the E. maxima fucoidan extract	988:1017	the E. maxima fucoidan extract	988:1017	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	6	39	dep	structure	768:776	arg1	the					764:766	the	764:766	the	764:766	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	1	40	theme	bioactive	188:196	arg1	compounds					198:206	beneficial bioactive compounds	177:206	beneficial bioactive compounds	177:206	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	4	41	from	maxima	517:522	arg1	extraction					498:507	The extraction	494:507	The extraction from E. maxima	494:522	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	4	42	theme	E.	514:515	arg1	maxima					517:522	E. maxima	514:522	E. maxima	514:522	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	7	43	theme	extract	1011:1017	arg1	presence					976:983	the presence	972:983	the presence of the E. maxima fucoidan extract	972:1017	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	4	44	contain	contain	566:572	arg2	L-fucose					587:594	4.45 ± 0.25% L-fucose	574:594	4.45 ± 0.25% L-fucose	574:594	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	4	44	contain	contain	566:572	arg1	fucoidan					538:545	6.89% fucoidan	532:545	6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate	532:620	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	4	44	contain	contain	566:572	arg2	sulphate					613:620	6.01 ± 0.53% sulphate	600:620	6.01 ± 0.53% sulphate	600:620	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	1	45	theme	compounds	198:206	arg1	maxima					110:115	Ecklonia maxima	101:115	Ecklonia maxima	101:115	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	1	45	theme	compounds	198:206	arg1	source					167:172	a potential source	155:172	a potential source of beneficial bioactive compounds	155:206	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	3	46	theme	E.	380:381	arg1	maxima					383:388	E. maxima	380:388	E. maxima	380:388	In this study, fucoidan was extracted from E. maxima by the hot water extraction method and then characterised by colorimetric assays for sugar composition.					
32070744	5	47	contain	had	662:664	arg2	weight					682:687	a low molecular weight	666:687	a low molecular weight of approximately 10 kDa	666:711	The water extracted E. maxima fucoidan had a low molecular weight of approximately 10 kDa.					
32070744	5	47	contain	had	662:664	arg1	fucoidan					653:660	The water extracted E. maxima fucoidan	623:660	The water extracted E. maxima fucoidan	623:660	The water extracted E. maxima fucoidan had a low molecular weight of approximately 10 kDa.					
32070744	5	48	theme	maxima	646:651	arg1	fucoidan					653:660	The water extracted E. maxima fucoidan	623:660	The water extracted E. maxima fucoidan	623:660	The water extracted E. maxima fucoidan had a low molecular weight of approximately 10 kDa.					
32070744	0	49	theme	powerful	35:42	arg1	α-glucosidase					86:98	α-glucosidase	86:98	α-glucosidase	86:98	Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.					
32070744	0	49	theme	powerful	35:42	arg1	Fucoidan					0:7	Fucoidan	0:7	Fucoidan from Ecklonia maxima	0:28	Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.					
32070744	0	49	theme	powerful	35:42	arg1	inhibitor					44:52	a powerful inhibitor	33:52	a powerful inhibitor of the diabetes-related enzyme	33:83	Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.					
32070744	7	50	theme	maxima	995:1000	arg1	extract					1011:1017	the E. maxima fucoidan extract	988:1017	the E. maxima fucoidan extract	988:1017	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	6	51	theme	fucoidan	799:806	arg1	integrity					782:790	integrity	782:790	integrity	782:790	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	6	51	theme	fucoidan	799:806	arg1	structure					768:776	structure	768:776	structure	768:776	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	3	52	theme	sugar	475:479	arg1	composition					481:491	sugar composition	475:491	sugar composition	475:491	In this study, fucoidan was extracted from E. maxima by the hot water extraction method and then characterised by colorimetric assays for sugar composition.					
32070744	8	53	with	α-glucosidase	1096:1108	arg1	range					1123:1127	an IC50 range	1115:1127	an IC50 range	1115:1127	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	8	53	with	α-glucosidase	1096:1108	arg1	lower					1175:1179	lower	1175:1179	lower	1175:1179	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	8	54	from	maxima	1037:1042	arg1	inhibitor					1083:1091	a potent mixed-type inhibitor	1063:1091	a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose	1063:1232	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	8	54	from	maxima	1037:1042	arg1	Fucoidan					1020:1027	Fucoidan	1020:1027	Fucoidan from E. maxima	1020:1042	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	2	55	theme	anti-diabetic	313:325	arg1	activity					327:334	anti-diabetic activity	313:334	anti-diabetic activity	313:334	Among these compounds, fucoidan, a sulphated polysaccharide has a wide range of bioactivities including anti-diabetic activity.					
32070744	7	56	dep	enzymes	913:919	arg1	enzymes					913:919	starch digestive enzymes	896:919	starch digestive enzymes; α-amylase and α-glucosidase	896:948	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	7	56	dep	enzymes	913:919	arg1	α-glucosidase					936:948	α-glucosidase	936:948	α-glucosidase	936:948	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	7	56	dep	enzymes	913:919	arg1	α-amylase					922:930	α-amylase	922:930	α-amylase	922:930	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	4	57	theme	%	585:585	arg1	L-fucose					587:594	4.45 ± 0.25% L-fucose	574:594	4.45 ± 0.25% L-fucose	574:594	The extraction from E. maxima yielded 6.89% fucoidan which was found to contain 4.45 ± 0.25% L-fucose and 6.01 ± 0.53% sulphate.					
32070744	6	58	theme	Structural	714:723	arg1	XRD					749:751	XRD	749:751	XRD	749:751	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	6	58	theme	Structural	714:723	arg1	NMR					741:743	NMR	741:743	NMR	741:743	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	6	58	theme	Structural	714:723	arg1	FT-IR					734:738	FT-IR	734:738	FT-IR	734:738	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	6	58	theme	Structural	714:723	arg1	studies					725:731	Structural studies	714:731	Structural studies (FT-IR, NMR and XRD)	714:752	Structural studies (FT-IR, NMR and XRD) confirmed the structure and integrity of the fucoidan to be similar to previously studied fucoidans in literature.					
32070744	1	59	theme	endemic	121:127	arg1	maxima					110:115	Ecklonia maxima	101:115	Ecklonia maxima	101:115	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	1	59	theme	endemic	121:127	arg1	seaweed					143:149	an endemic South African seaweed	118:149	an endemic South African seaweed	118:149	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	9	60	theme	powerful	1305:1312	arg1	inhibitor					1314:1322	a more powerful inhibitor	1298:1322	a more powerful inhibitor compared to some standard anti-diabetic compounds	1298:1372	Our present study demonstrated that fucoidan from E. maxima is a more powerful inhibitor compared to some standard anti-diabetic compounds and thus shows great potential for managing type 2 diabetes.					
32070744	9	60	theme	powerful	1305:1312	arg1	fucoidan					1271:1278	fucoidan	1271:1278	fucoidan from E. maxima	1271:1293	Our present study demonstrated that fucoidan from E. maxima is a more powerful inhibitor compared to some standard anti-diabetic compounds and thus shows great potential for managing type 2 diabetes.					
32070744	3	61	theme	colorimetric	451:462	arg1	assays					464:469	colorimetric assays	451:469	colorimetric assays for sugar composition	451:491	In this study, fucoidan was extracted from E. maxima by the hot water extraction method and then characterised by colorimetric assays for sugar composition.					
32070744	8	62	theme	mixed-type	1072:1081	arg1	inhibitor					1083:1091	a potent mixed-type inhibitor	1063:1091	a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose	1063:1232	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	8	62	theme	mixed-type	1072:1081	arg1	Fucoidan					1020:1027	Fucoidan	1020:1027	Fucoidan from E. maxima	1020:1042	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	8	63	theme	acarbose	1225:1232	arg1	inhibitor					1083:1091	a potent mixed-type inhibitor	1063:1091	a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose	1063:1232	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	8	63	theme	acarbose	1225:1232	arg1	Fucoidan					1020:1027	Fucoidan	1020:1027	Fucoidan from E. maxima	1020:1042	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	7	64	theme	E.	992:993	arg1	extract					1011:1017	the E. maxima fucoidan extract	988:1017	the E. maxima fucoidan extract	988:1017	Finally, the activities of starch digestive enzymes; α-amylase and α-glucosidase, were investigated in the presence of the E. maxima fucoidan extract.					
32070744	9	65	theme	anti-diabetic	1350:1362	arg1	compounds					1364:1372	some standard anti-diabetic compounds	1336:1372	some standard anti-diabetic compounds	1336:1372	Our present study demonstrated that fucoidan from E. maxima is a more powerful inhibitor compared to some standard anti-diabetic compounds and thus shows great potential for managing type 2 diabetes.					
32070744	8	66	theme	potent	1065:1070	arg1	inhibitor					1083:1091	a potent mixed-type inhibitor	1063:1091	a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose	1063:1232	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	8	66	theme	potent	1065:1070	arg1	Fucoidan					1020:1027	Fucoidan	1020:1027	Fucoidan from E. maxima	1020:1042	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	0	67	from	maxima	23:28	arg1	Fucoidan					0:7	Fucoidan	0:7	Fucoidan from Ecklonia maxima	0:28	Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.					
32070744	0	67	from	maxima	23:28	arg1	inhibitor					44:52	a powerful inhibitor	33:52	a powerful inhibitor of the diabetes-related enzyme	33:83	Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase.					
32070744	8	68	theme	0.27-0.31 mg.ml-1	1132:1148	arg1	range					1123:1127	an IC50 range	1115:1127	an IC50 range	1115:1127	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	8	68	theme	0.27-0.31 mg.ml-1	1132:1148	arg1	lower					1175:1179	lower	1175:1179	lower	1175:1179	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32070744	1	69	theme	African	135:141	arg1	maxima					110:115	Ecklonia maxima	101:115	Ecklonia maxima	101:115	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	1	69	theme	African	135:141	arg1	seaweed					143:149	an endemic South African seaweed	118:149	an endemic South African seaweed	118:149	Ecklonia maxima, an endemic South African seaweed, is a potential source of beneficial bioactive compounds.					
32070744	8	70	theme	α-glucosidase	1096:1108	arg1	acarbose					1225:1232	α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose	1096:1232	α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose	1096:1232	Fucoidan from E. maxima was observed to be a potent mixed-type inhibitor of α-glucosidase with an IC50 range of 0.27-0.31 mg.ml-1, which was significantly lower than the commercial anti-diabetic standard, acarbose.					
32798543	0	0	theme	drug	80:83	arg1	delivery					85:92	drug delivery	80:92	drug delivery	80:92	Doubly crosslinked biodegradable hydrogels based on gellan gum and chitosan for drug delivery and wound dressing.					
32798543	2	1	theme	crosslinked	484:494	arg1	hydrogel					496:503	a doubly crosslinked hydrogel	475:503	a doubly crosslinked hydrogel	475:503	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	2	2	theme	gellan	568:573	arg1	gum					575:577	oxidized gellan gum	559:577	oxidized gellan gum (OG) (pre-crosslinked by calcium ion)	559:615	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	2	2	theme	gellan	568:573	arg1	OG					580:581	OG	580:581	OG	580:581	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	1	3	theme	release	161:167	arg1	capability					169:178	sustained drug release capability	146:178	sustained drug release capability	146:178	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	2	4	dep	microspheres	451:462	arg1	hydrogel					496:503	a doubly crosslinked hydrogel	475:503	a doubly crosslinked hydrogel	475:503	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	7	5	theme	injectable	1519:1528	arg1	scaffold					1530:1537	a promising injectable scaffold	1507:1537	a promising injectable scaffold for promoting wound regeneration and drug delivery	1507:1588	These results support the bioactive composite hydrogel can be employed as a promising injectable scaffold for promoting wound regeneration and drug delivery.					
32798543	2	6	theme	hydrogel	352:359	arg1	type					319:322	a new type	313:322	a new type of composite wound dressing hydrogel aiming at avoiding wound infection	313:394	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	7	7	dep	support	1447:1453	arg1	employed					1495:1502	employed	1495:1502	support the bioactive composite hydrogel can be employed as a promising injectable scaffold for promoting wound regeneration and drug delivery	1447:1588	These results support the bioactive composite hydrogel can be employed as a promising injectable scaffold for promoting wound regeneration and drug delivery.					
32798543	5	8	theme	hydrogel	1180:1187	arg1	performance					1155:1165	the aforementioned performance	1136:1165	the aforementioned performance of the blank hydrogel	1136:1187	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	2	9	theme	chitosan	635:642	arg1	crosslinking					543:554	Schiff-base crosslinking	531:554	Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS)	531:649	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	3	10	theme	w/w	828:830	arg1	ratio					833:837	fixed OG/calcium (w/w) ratio	810:837	fixed OG/calcium (w/w) ratio	810:837	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	1	11	theme	wound	283:287	arg1	healing					289:295	wound healing	283:295	wound healing	283:295	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	3	12	with	ratios	781:786	arg1	ratio					833:837	fixed OG/calcium (w/w) ratio	810:837	fixed OG/calcium (w/w) ratio	810:837	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	4	13	theme	gelation	939:946	arg1	time					948:951	a 139 s gelation time	931:951	a 139 s gelation time	931:951	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	4	13	theme	gelation	939:946	arg1	index					963:967	swelling index	954:967	swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus	954:1089	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	0	14	link	crosslinked	7:17	arg1	hydrogels					33:41	Doubly crosslinked biodegradable hydrogels	0:41	Doubly crosslinked biodegradable hydrogels	0:41	Doubly crosslinked biodegradable hydrogels based on gellan gum and chitosan for drug delivery and wound dressing.					
32798543	5	15	theme	sustained	1211:1219	arg1	release					1221:1227	the sustained release	1207:1227	the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine)	1207:1312	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	3	16	theme	CMCS/OG	791:797	arg1	ratios					781:786	the ratios	777:786	the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio	777:837	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	4	17	from	equilibrium	1002:1012	arg1	day					1054:1056	the seventh day	1042:1056	the seventh day	1042:1056	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	1	18	with	hydrogels	131:139	arg1	activity					198:205	antibacterial activity	184:205	antibacterial activity	184:205	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	1	18	with	hydrogels	131:139	arg1	capability					169:178	sustained drug release capability	146:178	sustained drug release capability	146:178	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	2	19	theme	carboxymethyl	621:633	arg1	CMCS					645:648	CMCS	645:648	CMCS	645:648	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	2	19	theme	carboxymethyl	621:633	arg1	chitosan					635:642	carboxymethyl chitosan	621:642	carboxymethyl chitosan (CMCS)	621:649	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	7	20	theme	wound	1553:1557	arg1	regeneration					1559:1570	wound regeneration	1553:1570	wound regeneration	1553:1570	These results support the bioactive composite hydrogel can be employed as a promising injectable scaffold for promoting wound regeneration and drug delivery.					
32798543	5	21	theme	cargoes	1232:1238	arg1	release					1221:1227	the sustained release	1207:1227	the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine)	1207:1312	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	0	22	theme	wound	98:102	arg1	dressing					104:111	wound dressing	98:111	wound dressing	98:111	Doubly crosslinked biodegradable hydrogels based on gellan gum and chitosan for drug delivery and wound dressing.					
32798543	6	23	theme	excellent	1342:1350	arg1	activity					1366:1373	the excellent antibacterial activity	1338:1373	the excellent antibacterial activity of the composite hydrogel	1338:1399	Moreover, the excellent antibacterial activity of the composite hydrogel was also demonstrated in vitro.					
32798543	4	24	theme	seventh	1046:1052	arg1	day					1054:1056	the seventh day	1042:1056	the seventh day	1042:1056	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	1	25	theme	antibacterial	184:196	arg1	activity					198:205	antibacterial activity	184:205	antibacterial activity	184:205	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	0	26	theme	crosslinked	7:17	arg1	hydrogels					33:41	Doubly crosslinked biodegradable hydrogels	0:41	Doubly crosslinked biodegradable hydrogels	0:41	Doubly crosslinked biodegradable hydrogels based on gellan gum and chitosan for drug delivery and wound dressing.					
32798543	3	27	theme	degradation	687:697	arg1	rate					699:702	degradation rate	687:702	degradation rate	687:702	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	4	28	from	rate	1034:1037	arg1	day					1054:1056	the seventh day	1042:1056	the seventh day	1042:1056	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	5	29	theme	antibacterial	1241:1253	arg1	drugs					1255:1259	antibacterial drugs	1241:1259	antibacterial drugs	1241:1259	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	5	29	theme	antibacterial	1241:1253	arg1	hydrochloride					1275:1287	tetracycline hydrochloride	1262:1287	tetracycline hydrochloride	1262:1287	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	5	29	theme	antibacterial	1241:1253	arg1	sulfadiazine					1300:1311	silver sulfadiazine	1293:1311	silver sulfadiazine	1293:1311	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	4	30	theme	139 s	933:937	arg1	time					948:951	a 139 s gelation time	931:951	a 139 s gelation time	931:951	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	4	30	theme	139 s	933:937	arg1	index					963:967	swelling index	954:967	swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus	954:1089	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	2	31	theme	calcium	604:610	arg1	ion					612:614	calcium ion	604:614	calcium ion	604:614	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	2	32	theme	new	315:317	arg1	type					319:322	a new type	313:322	a new type of composite wound dressing hydrogel aiming at avoiding wound infection	313:394	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	4	33	theme	100.5	1015:1019	arg1	%					1020:1020	%	1020:1020	%	1020:1020	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	2	34	theme	wound	380:384	arg1	infection					386:394	avoiding wound infection	371:394	avoiding wound infection	371:394	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	2	35	theme	gum	447:449	arg1	microspheres					451:462	gellan gum microspheres	440:462	gellan gum microspheres (GMs)	440:468	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	2	35	theme	gum	447:449	arg1	GMs					465:467	GMs	465:467	GMs	465:467	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	7	36	theme	drug	1576:1579	arg1	delivery					1581:1588	drug delivery	1576:1588	drug delivery	1576:1588	These results support the bioactive composite hydrogel can be employed as a promising injectable scaffold for promoting wound regeneration and drug delivery.					
32798543	2	37	theme	oxidized	559:566	arg1	gum					575:577	oxidized gellan gum	559:577	oxidized gellan gum (OG) (pre-crosslinked by calcium ion)	559:615	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	2	37	theme	oxidized	559:566	arg1	OG					580:581	OG	580:581	OG	580:581	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	2	38	theme	gellan	440:445	arg1	microspheres					451:462	gellan gum microspheres	440:462	gellan gum microspheres (GMs)	440:468	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	2	38	theme	gellan	440:445	arg1	GMs					465:467	GMs	465:467	GMs	465:467	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	3	39	theme	mechanical	708:717	arg1	properties					719:728	mechanical properties	708:728	mechanical properties of the blank hydrogel	708:750	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	2	40	theme	avoiding	371:378	arg1	infection					386:394	avoiding wound infection	371:394	avoiding wound infection	371:394	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	4	41	theme	degradation	1022:1032	arg1	rate					1034:1037	100.5% degradation rate	1015:1037	100.5% degradation rate on the seventh day	1015:1056	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	1	42	theme	great	222:226	arg1	potential					228:236	great potential	222:236	great potential	222:236	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	5	43	dep	cargoes	1232:1238	arg1	drugs					1255:1259	antibacterial drugs	1241:1259	antibacterial drugs	1241:1259	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	5	43	dep	cargoes	1232:1238	arg1	hydrochloride					1275:1287	tetracycline hydrochloride	1262:1287	tetracycline hydrochloride	1262:1287	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	5	43	dep	cargoes	1232:1238	arg1	sulfadiazine					1300:1311	silver sulfadiazine	1293:1311	silver sulfadiazine	1293:1311	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	2	44	theme	wound	337:341	arg1	hydrogel					352:359	composite wound dressing hydrogel	327:359	composite wound dressing hydrogel aiming at avoiding wound infection	327:394	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	4	45	theme	hydrogel	876:883	arg1	performance					857:867	The best overall performance	840:867	The best overall performance of the hydrogel	840:883	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	7	46	theme	bioactive	1459:1467	arg1	hydrogel					1479:1486	the bioactive composite hydrogel	1455:1486	the bioactive composite hydrogel	1455:1486	These results support the bioactive composite hydrogel can be employed as a promising injectable scaffold for promoting wound regeneration and drug delivery.					
32798543	5	47	theme	silver	1293:1298	arg1	drugs					1255:1259	antibacterial drugs	1241:1259	antibacterial drugs	1241:1259	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	5	47	theme	silver	1293:1298	arg1	sulfadiazine					1300:1311	silver sulfadiazine	1293:1311	silver sulfadiazine	1293:1311	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	4	48	theme	%	1020:1020	arg1	rate					1034:1037	100.5% degradation rate	1015:1037	100.5% degradation rate on the seventh day	1015:1056	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	5	49	theme	tetracycline	1262:1273	arg1	drugs					1255:1259	antibacterial drugs	1241:1259	antibacterial drugs	1241:1259	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	5	49	theme	tetracycline	1262:1273	arg1	hydrochloride					1275:1287	tetracycline hydrochloride	1262:1287	tetracycline hydrochloride	1262:1287	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	6	50	theme	antibacterial	1352:1364	arg1	activity					1366:1373	the excellent antibacterial activity	1338:1373	the excellent antibacterial activity of the composite hydrogel	1338:1399	Moreover, the excellent antibacterial activity of the composite hydrogel was also demonstrated in vitro.					
32798543	2	51	theme	Schiff-base	531:541	arg1	crosslinking					543:554	Schiff-base crosslinking	531:554	Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS)	531:649	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	2	52	link	crosslinked	484:494	arg1	hydrogel					496:503	a doubly crosslinked hydrogel	475:503	a doubly crosslinked hydrogel	475:503	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	5	53	theme	aforementioned	1140:1153	arg1	performance					1155:1165	the aforementioned performance	1136:1165	the aforementioned performance of the blank hydrogel	1136:1187	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	4	54	from	modulus	1083:1089	arg1	day					1054:1056	the seventh day	1042:1056	the seventh day	1042:1056	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	4	55	theme	compressive	1071:1081	arg1	modulus					1083:1089	8.8 KPa compressive modulus	1063:1089	8.8 KPa compressive modulus	1063:1089	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	2	56	theme	dressing	343:350	arg1	hydrogel					352:359	composite wound dressing hydrogel	327:359	composite wound dressing hydrogel aiming at avoiding wound infection	327:394	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	0	57	theme	gellan	52:57	arg1	gum					59:61	gellan gum	52:61	gellan gum	52:61	Doubly crosslinked biodegradable hydrogels based on gellan gum and chitosan for drug delivery and wound dressing.					
32798543	3	58	theme	blank	737:741	arg1	hydrogel					743:750	the blank hydrogel	733:750	the blank hydrogel	733:750	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	5	59	with	embedded	1104:1111	arg1	GMs					1131:1133	cargo-loaded GMs	1118:1133	cargo-loaded GMs	1118:1133	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	4	60	with	16/7	914:917	arg1	time					948:951	a 139 s gelation time	931:951	a 139 s gelation time	931:951	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	4	60	with	16/7	914:917	arg1	index					963:967	swelling index	954:967	swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus	954:1089	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	1	61	theme	Biopolymer-based	114:129	arg1	hydrogels					131:139	Biopolymer-based hydrogels	114:139	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity	114:205	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	3	62	theme	fixed	810:814	arg1	ratio					833:837	fixed OG/calcium (w/w) ratio	810:837	fixed OG/calcium (w/w) ratio	810:837	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	3	63	theme	hydrogel	743:750	arg1	time					665:668	The gelation time	652:668	The gelation time	652:668	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	3	63	theme	hydrogel	743:750	arg1	index					680:684	swelling index	671:684	swelling index	671:684	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	3	63	theme	hydrogel	743:750	arg1	rate					699:702	degradation rate	687:702	degradation rate	687:702	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	3	63	theme	hydrogel	743:750	arg1	properties					719:728	mechanical properties	708:728	mechanical properties of the blank hydrogel	708:750	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	2	64	theme	gum	575:577	arg1	crosslinking					543:554	Schiff-base crosslinking	531:554	Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS)	531:649	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	3	65	theme	gelation	656:663	arg1	time					665:668	The gelation time	652:668	The gelation time	652:668	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	5	66	theme	cargo-loaded	1118:1129	arg1	GMs					1131:1133	cargo-loaded GMs	1118:1133	cargo-loaded GMs	1118:1133	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	2	67	theme	composite	327:335	arg1	hydrogel					352:359	composite wound dressing hydrogel	327:359	composite wound dressing hydrogel aiming at avoiding wound infection	327:394	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	3	68	theme	OG/calcium	816:825	arg1	ratio					833:837	fixed OG/calcium (w/w) ratio	810:837	fixed OG/calcium (w/w) ratio	810:837	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	1	69	theme	clinical	241:248	arg1	application					250:260	clinical application	241:260	clinical application in drug delivery and wound healing	241:295	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	1	70	from	application	250:260	arg1	delivery					270:277	drug delivery	265:277	drug delivery	265:277	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	1	70	from	application	250:260	arg1	healing					289:295	wound healing	283:295	wound healing	283:295	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	4	71	theme	swelling	954:961	arg1	time					948:951	a 139 s gelation time	931:951	a 139 s gelation time	931:951	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	4	71	theme	swelling	954:961	arg1	index					963:967	swelling index	954:967	swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus	954:1089	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	7	72	theme	promising	1509:1517	arg1	scaffold					1530:1537	a promising injectable scaffold	1507:1537	a promising injectable scaffold for promoting wound regeneration and drug delivery	1507:1588	These results support the bioactive composite hydrogel can be employed as a promising injectable scaffold for promoting wound regeneration and drug delivery.					
32798543	2	73	dep	gum	575:577	arg1	pre-crosslinked					585:599	pre-crosslinked	585:599	pre-crosslinked	585:599	In this study, a new type of composite wound dressing hydrogel aiming at avoiding wound infection was developed through embedding drug loaded gellan gum microspheres (GMs) into a doubly crosslinked hydrogel, which was constructed by Schiff-base crosslinking of oxidized gellan gum (OG) (pre-crosslinked by calcium ion) and carboxymethyl chitosan (CMCS).					
32798543	5	74	theme	blank	1174:1178	arg1	hydrogel					1180:1187	the blank hydrogel	1170:1187	the blank hydrogel	1170:1187	After being embedded with cargo-loaded GMs, the aforementioned performance of the blank hydrogel was improved, and the sustained release of cargoes (antibacterial drugs, tetracycline hydrochloride and silver sulfadiazine) was observed.					
32798543	1	75	theme	sustained	146:154	arg1	capability					169:178	sustained drug release capability	146:178	sustained drug release capability	146:178	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	6	76	theme	hydrogel	1392:1399	arg1	activity					1366:1373	the excellent antibacterial activity	1338:1373	the excellent antibacterial activity of the composite hydrogel	1338:1399	Moreover, the excellent antibacterial activity of the composite hydrogel was also demonstrated in vitro.					
32798543	4	77	theme	overall	849:855	arg1	performance					857:867	The best overall performance	840:867	The best overall performance of the hydrogel	840:883	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	7	78	theme	composite	1469:1477	arg1	hydrogel					1479:1486	the bioactive composite hydrogel	1455:1486	the bioactive composite hydrogel	1455:1486	These results support the bioactive composite hydrogel can be employed as a promising injectable scaffold for promoting wound regeneration and drug delivery.					
32798543	3	79	theme	swelling	671:678	arg1	index					680:684	swelling index	671:684	swelling index	671:684	The gelation time, swelling index, degradation rate and mechanical properties of the blank hydrogel was optimized by varying the ratios of CMCS/OG (w/w) with fixed OG/calcium (w/w) ratio.					
32798543	0	80	theme	biodegradable	19:31	arg1	hydrogels					33:41	Doubly crosslinked biodegradable hydrogels	0:41	Doubly crosslinked biodegradable hydrogels	0:41	Doubly crosslinked biodegradable hydrogels based on gellan gum and chitosan for drug delivery and wound dressing.					
32798543	4	81	theme	8.8 KPa	1063:1069	arg1	modulus					1083:1089	8.8 KPa compressive modulus	1063:1089	8.8 KPa compressive modulus	1063:1089	The best overall performance of the hydrogel was obtained when CMCS/OG is 16/7 (w/w), with a 139 s gelation time, swelling index remained above 30 after swelling equilibrium, 100.5% degradation rate on the seventh day, and 8.8 KPa compressive modulus.					
32798543	1	82	theme	drug	156:159	arg1	capability					169:178	sustained drug release capability	146:178	sustained drug release capability	146:178	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
32798543	6	83	theme	composite	1382:1390	arg1	hydrogel					1392:1399	the composite hydrogel	1378:1399	the composite hydrogel	1378:1399	Moreover, the excellent antibacterial activity of the composite hydrogel was also demonstrated in vitro.					
32798543	1	84	theme	drug	265:268	arg1	delivery					270:277	drug delivery	265:277	drug delivery	265:277	Biopolymer-based hydrogels with sustained drug release capability and antibacterial activity have exhibited great potential in clinical application in drug delivery and wound healing.					
33450706	8	0	theme	composite	1312:1320	arg1	hydrogels					1322:1330	composite hydrogels	1312:1330	composite hydrogels could be potentially applied in antibacterial biological and food packaging area	1312:1411	The obtained CNFs reinforced composite hydrogels could be potentially applied in antibacterial biological and food packaging area.					
33450706	7	1	from	enhancement	1133:1143	arg1	composite					1148:1156	composite	1148:1156	composite	1148:1156	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	7	2	theme	mechanical	1104:1113	arg1	enhancement					1133:1143	an excellent mechanical and antibacterial enhancement	1091:1143	an excellent mechanical and antibacterial enhancement in composite	1091:1156	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	3	3	theme	pH	485:486	arg1	sensitivity					488:498	pH sensitivity	485:498	pH sensitivity	485:498	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	4	4	theme	reinforced	578:587	arg1	particle					589:596	normal reinforced particle	571:596	normal reinforced particle	571:596	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	0	5	theme	composite	101:109	arg1	hydrogels					111:119	chitosan-based composite hydrogels	86:119	chitosan-based composite hydrogels	86:119	Preparation and characterization of apoacynum venetum cellulose nanofibers reinforced chitosan-based composite hydrogels.					
33450706	7	6	theme	excellent	1094:1102	arg1	enhancement					1133:1143	an excellent mechanical and antibacterial enhancement	1091:1143	an excellent mechanical and antibacterial enhancement in composite	1091:1156	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	5	7	theme	%	892:892	arg1	CNFs					894:897	1.5vt% CNFs	887:897	1.5vt% CNFs	887:897	And the compressive strength increases nearly 20 % and swelling capacity achieve 140 %, when 1.5vt% CNFs added.					
33450706	5	8	dep	increases	823:831	arg1	%					843:843	%	843:843	%	843:843	And the compressive strength increases nearly 20 % and swelling capacity achieve 140 %, when 1.5vt% CNFs added.					
33450706	4	9	theme	normal	571:576	arg1	particle					589:596	normal reinforced particle	571:596	normal reinforced particle	571:596	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	3	10	from	sensitivity	488:498	arg1	capability					473:482	mechanical, swelling capability	452:482	mechanical, swelling capability	452:482	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	5	11	theme	1.5vt	887:891	arg1	%					892:892	%	892:892	%	892:892	And the compressive strength increases nearly 20 % and swelling capacity achieve 140 %, when 1.5vt% CNFs added.					
33450706	8	12	theme	biological	1378:1387	arg1	area					1408:1411	antibacterial biological and food packaging area	1364:1411	antibacterial biological and food packaging area	1364:1411	The obtained CNFs reinforced composite hydrogels could be potentially applied in antibacterial biological and food packaging area.					
33450706	3	13	theme	CNFs	381:384	arg1	effect					371:376	The effect	367:376	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial	367:516	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	4	14	theme	permanent	758:766	arg1	damage					768:773	defer permanent damage	752:773	defer permanent damage	752:773	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	7	15	theme	different	1224:1232	arg1	shapes					1234:1239	the different shapes	1220:1239	the different shapes	1220:1239	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	4	16	theme	chitosan	705:712	arg1	hydrogel					714:721	chitosan hydrogel	705:721	chitosan hydrogel	705:721	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	4	17	theme	hydrogen	642:649	arg1	bonding					651:657	abundant hydrogen bonding	633:657	abundant hydrogen bonding	633:657	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	3	18	from	antibacterial	504:516	arg1	capability					473:482	mechanical, swelling capability	452:482	mechanical, swelling capability	452:482	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	4	19	theme	interconnected	668:681	arg1	structure					690:698	interconnected porous structure	668:698	interconnected porous structure	668:698	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	5	20	theme	compressive	802:812	arg1	increases					823:831	the compressive strength increases	798:831	the compressive strength increases nearly 20 %	798:843	And the compressive strength increases nearly 20 % and swelling capacity achieve 140 %, when 1.5vt% CNFs added.					
33450706	6	21	theme	antibacterial	991:1003	arg1	rate					1005:1008	the antibacterial rate	987:1008	the antibacterial rate against E. coli and S. aureus of composite hydrogel	987:1060	Thanks to CNFs which obtained from apocynum venetum generated positive effect on the antibacterial rate against E. coli and S. aureus of composite hydrogel.					
33450706	6	22	from	effect	977:982	arg1	rate					1005:1008	the antibacterial rate	987:1008	the antibacterial rate against E. coli and S. aureus of composite hydrogel	987:1060	Thanks to CNFs which obtained from apocynum venetum generated positive effect on the antibacterial rate against E. coli and S. aureus of composite hydrogel.					
33450706	5	23	theme	strength	814:821	arg1	increases					823:831	the compressive strength increases	798:831	the compressive strength increases nearly 20 %	798:843	And the compressive strength increases nearly 20 % and swelling capacity achieve 140 %, when 1.5vt% CNFs added.					
33450706	7	24	theme	rational	1198:1205	arg1	selection					1207:1215	the rational selection	1194:1215	the rational selection of the different shapes	1194:1239	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	7	25	theme	materials	1272:1280	arg1	prospect					1181:1188	a new prospect	1175:1188	a new prospect for the rational selection of the different shapes	1175:1239	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	7	25	theme	materials	1272:1280	arg1	ratio					1252:1256	aspect ratio	1245:1256	aspect ratio of reinforced materials	1245:1280	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	8	26	theme	antibacterial	1364:1376	arg1	area					1408:1411	antibacterial biological and food packaging area	1364:1411	antibacterial biological and food packaging area	1364:1411	The obtained CNFs reinforced composite hydrogels could be potentially applied in antibacterial biological and food packaging area.					
33450706	6	27	theme	hydrogel	1053:1060	arg1	rate					1005:1008	the antibacterial rate	987:1008	the antibacterial rate against E. coli and S. aureus of composite hydrogel	987:1060	Thanks to CNFs which obtained from apocynum venetum generated positive effect on the antibacterial rate against E. coli and S. aureus of composite hydrogel.					
33450706	1	28	theme	mechanical	207:216	arg1	properties					218:227	the mechanical properties	203:227	the mechanical properties of composites	203:241	Addition of nanomaterials into polymer matrix is a promising avenue to reinforce the mechanical properties of composites.					
33450706	6	29	theme	positive	968:975	arg1	effect					977:982	positive effect	968:982	positive effect on the antibacterial rate against E. coli and S. aureus of composite hydrogel	968:1060	Thanks to CNFs which obtained from apocynum venetum generated positive effect on the antibacterial rate against E. coli and S. aureus of composite hydrogel.					
33450706	6	30	theme	composite	1043:1051	arg1	hydrogel					1053:1060	composite hydrogel	1043:1060	composite hydrogel	1043:1060	Thanks to CNFs which obtained from apocynum venetum generated positive effect on the antibacterial rate against E. coli and S. aureus of composite hydrogel.					
33450706	5	31	theme	swelling	849:856	arg1	capacity					858:865	swelling capacity	849:865	swelling capacity	849:865	And the compressive strength increases nearly 20 % and swelling capacity achieve 140 %, when 1.5vt% CNFs added.					
33450706	4	32	theme	defer	752:756	arg1	damage					768:773	defer permanent damage	752:773	defer permanent damage	752:773	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	8	33	theme	packaging	1398:1406	arg1	area					1408:1411	antibacterial biological and food packaging area	1364:1411	antibacterial biological and food packaging area	1364:1411	The obtained CNFs reinforced composite hydrogels could be potentially applied in antibacterial biological and food packaging area.					
33450706	8	34	dep	hydrogels	1322:1330	arg1	applied					1353:1359	applied	1353:1359	could be potentially applied in antibacterial biological and food packaging area	1332:1411	The obtained CNFs reinforced composite hydrogels could be potentially applied in antibacterial biological and food packaging area.					
33450706	1	35	theme	nanomaterials	134:146	arg1	Addition					122:129	Addition	122:129	Addition of nanomaterials into polymer matrix	122:166	Addition of nanomaterials into polymer matrix is a promising avenue to reinforce the mechanical properties of composites.					
33450706	1	35	theme	nanomaterials	134:146	arg1	avenue					183:188	a promising avenue	171:188	a promising avenue to reinforce the mechanical properties of composites	171:241	Addition of nanomaterials into polymer matrix is a promising avenue to reinforce the mechanical properties of composites.					
33450706	1	36	theme	composites	232:241	arg1	properties					218:227	the mechanical properties	203:227	the mechanical properties of composites	203:241	Addition of nanomaterials into polymer matrix is a promising avenue to reinforce the mechanical properties of composites.					
33450706	0	37	theme	venetum	46:52	arg1	nanofibers					64:73	apoacynum venetum cellulose nanofibers	36:73	apoacynum venetum cellulose nanofibers	36:73	Preparation and characterization of apoacynum venetum cellulose nanofibers reinforced chitosan-based composite hydrogels.					
33450706	7	38	theme	reinforced	1261:1270	arg1	materials					1272:1280	reinforced materials	1261:1280	reinforced materials	1261:1280	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	3	39	from	effect	371:376	arg1	relationship					436:447	the structure-property relationship	413:447	the structure-property relationship in mechanical, swelling capability	413:482	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	3	39	from	effect	371:376	arg1	antibacterial					504:516	antibacterial	504:516	antibacterial	504:516	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	3	39	from	effect	371:376	arg1	sensitivity					488:498	pH sensitivity	485:498	pH sensitivity	485:498	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	2	40	theme	chitosan-based	258:271	arg1	matrix					359:364	matrix	359:364	matrix	359:364	In this work, chitosan-based hydrogels reinforced by cellulose nanofibers (CNFs) have been prepared by chitosan as matrix.					
33450706	2	40	theme	chitosan-based	258:271	arg1	hydrogels					273:281	chitosan-based hydrogels	258:281	chitosan-based hydrogels reinforced by cellulose nanofibers (CNFs)	258:323	In this work, chitosan-based hydrogels reinforced by cellulose nanofibers (CNFs) have been prepared by chitosan as matrix.					
33450706	3	41	theme	structure-property	417:434	arg1	relationship					436:447	the structure-property relationship	413:447	the structure-property relationship in mechanical, swelling capability	413:482	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	0	42	theme	apoacynum	36:44	arg1	nanofibers					64:73	apoacynum venetum cellulose nanofibers	36:73	apoacynum venetum cellulose nanofibers	36:73	Preparation and characterization of apoacynum venetum cellulose nanofibers reinforced chitosan-based composite hydrogels.					
33450706	4	43	from	particle	589:596	arg1	Different					556:564	Different	556:564	Different	556:564	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	8	44	theme	food	1393:1396	arg1	area					1408:1411	antibacterial biological and food packaging area	1364:1411	antibacterial biological and food packaging area	1364:1411	The obtained CNFs reinforced composite hydrogels could be potentially applied in antibacterial biological and food packaging area.					
33450706	4	45	theme	abundant	633:640	arg1	bonding					651:657	abundant hydrogen bonding	633:657	abundant hydrogen bonding	633:657	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	0	46	theme	nanofibers	64:73	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of apoacynum venetum cellulose nanofibers reinforced chitosan-based composite hydrogels.					
33450706	0	46	theme	nanofibers	64:73	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of apoacynum venetum cellulose nanofibers reinforced chitosan-based composite hydrogels.					
33450706	6	47	dep	obtained	927:934	arg1	generated					958:966	generated	958:966	generated positive effect on the antibacterial rate against E. coli and S. aureus of composite hydrogel	958:1060	Thanks to CNFs which obtained from apocynum venetum generated positive effect on the antibacterial rate against E. coli and S. aureus of composite hydrogel.					
33450706	3	48	from	relationship	436:447	arg1	capability					473:482	mechanical, swelling capability	452:482	mechanical, swelling capability	452:482	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	3	49	theme	mechanical	452:461	arg1	capability					473:482	mechanical, swelling capability	452:482	mechanical, swelling capability	452:482	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	0	50	theme	cellulose	54:62	arg1	nanofibers					64:73	apoacynum venetum cellulose nanofibers	36:73	apoacynum venetum cellulose nanofibers	36:73	Preparation and characterization of apoacynum venetum cellulose nanofibers reinforced chitosan-based composite hydrogels.					
33450706	7	51	theme	new	1177:1179	arg1	prospect					1181:1188	a new prospect	1175:1188	a new prospect for the rational selection of the different shapes	1175:1239	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	4	52	with	CNFs	599:602	arg1	shape					623:627	special fiber shape	609:627	special fiber shape	609:627	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	4	52	with	CNFs	599:602	arg1	bonding					651:657	abundant hydrogen bonding	633:657	abundant hydrogen bonding	633:657	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	1	53	theme	polymer	153:159	arg1	matrix					161:166	polymer matrix	153:166	polymer matrix	153:166	Addition of nanomaterials into polymer matrix is a promising avenue to reinforce the mechanical properties of composites.					
33450706	2	54	theme	cellulose	297:305	arg1	CNFs					319:322	CNFs	319:322	CNFs	319:322	In this work, chitosan-based hydrogels reinforced by cellulose nanofibers (CNFs) have been prepared by chitosan as matrix.					
33450706	2	54	theme	cellulose	297:305	arg1	nanofibers					307:316	cellulose nanofibers	297:316	cellulose nanofibers (CNFs)	297:323	In this work, chitosan-based hydrogels reinforced by cellulose nanofibers (CNFs) have been prepared by chitosan as matrix.					
33450706	7	55	theme	aspect	1245:1250	arg1	ratio					1252:1256	aspect ratio	1245:1256	aspect ratio of reinforced materials	1245:1280	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	3	56	dep	CNFs	381:384	arg1	%					390:390	1vt%	387:390	1vt%	387:390	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	3	56	dep	CNFs	381:384	arg1	%					398:398	1.5vt%	393:398	1.5vt%	393:398	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	3	56	dep	CNFs	381:384	arg1	%					407:407	2vt%	404:407	2vt%	404:407	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	4	57	theme	porous	683:688	arg1	structure					690:698	interconnected porous structure	668:698	interconnected porous structure	668:698	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	8	58	theme	obtained	1287:1294	arg1	CNFs					1296:1299	The obtained CNFs	1283:1299	The obtained CNFs	1283:1299	The obtained CNFs reinforced composite hydrogels could be potentially applied in antibacterial biological and food packaging area.					
33450706	0	59	theme	chitosan-based	86:99	arg1	hydrogels					111:119	chitosan-based composite hydrogels	86:119	chitosan-based composite hydrogels	86:119	Preparation and characterization of apoacynum venetum cellulose nanofibers reinforced chitosan-based composite hydrogels.					
33450706	5	60	dep	%	843:843	arg1	20					840:841	20	840:841	20	840:841	And the compressive strength increases nearly 20 % and swelling capacity achieve 140 %, when 1.5vt% CNFs added.					
33450706	7	61	theme	shapes	1234:1239	arg1	selection					1207:1215	the rational selection	1194:1215	the rational selection of the different shapes	1194:1239	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	4	62	theme	fiber	617:621	arg1	shape					623:627	special fiber shape	609:627	special fiber shape	609:627	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33450706	7	63	theme	antibacterial	1119:1131	arg1	enhancement					1133:1143	an excellent mechanical and antibacterial enhancement	1091:1143	an excellent mechanical and antibacterial enhancement in composite	1091:1156	It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials.					
33450706	3	64	dep	mechanical	452:461	arg1	swelling					464:471	swelling	464:471	swelling	464:471	The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively.					
33450706	1	65	theme	promising	173:181	arg1	Addition					122:129	Addition	122:129	Addition of nanomaterials into polymer matrix	122:166	Addition of nanomaterials into polymer matrix is a promising avenue to reinforce the mechanical properties of composites.					
33450706	1	65	theme	promising	173:181	arg1	avenue					183:188	a promising avenue	171:188	a promising avenue to reinforce the mechanical properties of composites	171:241	Addition of nanomaterials into polymer matrix is a promising avenue to reinforce the mechanical properties of composites.					
33450706	4	66	theme	special	609:615	arg1	shape					623:627	special fiber shape	609:627	special fiber shape	609:627	Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing.					
33933541	1	0	with	polysaccharide	163:176	arg1	weight					201:206	a molecular weight	189:206	a molecular weight of 1.37 × 105 Da	189:223	A novel polysaccharide (GLP) with a molecular weight of 1.37 × 105 Da was purified from the roots of G. littoralis.					
33933541	0	1	from	activities	43:52	arg1	preparation					127:137	preparation	127:137	preparation of nano-silver	127:152	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	0	1	from	activities	43:52	arg1	littoralis					93:102	Glehnia littoralis	85:102	Glehnia littoralis	85:102	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	8	2	theme	silver	1201:1206	arg1	AgNPs					1223:1227	AgNPs	1223:1227	AgNPs	1223:1227	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	8	2	theme	silver	1201:1206	arg1	nanoparticles					1208:1220	silver nanoparticles	1201:1220	silver nanoparticles (AgNPs)	1201:1228	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	2	3	dep	linkage	419:425	arg1	4					397:397	4	397:397	4	397:397	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	4	theme	glycoside	409:417	arg1	structure					353:361	the structure	349:361	the structure of GLP	349:368	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	4	theme	glycoside	409:417	arg1	linkage					419:425	a 1 → 4)-α-D-Glcp glycoside linkage	391:425	a 1 → 4)-α-D-Glcp glycoside linkage	391:425	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	0	5	from	characterization	11:26	arg1	preparation					127:137	preparation	127:137	preparation of nano-silver	127:152	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	0	5	from	characterization	11:26	arg1	littoralis					93:102	Glehnia littoralis	85:102	Glehnia littoralis	85:102	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	3	6	theme	nano-precipitation	538:555	arg1	method					557:562	nano-precipitation method	538:562	nano-precipitation method	538:562	Then, GLP-NPs were prepared by nano-precipitation method, the particle size of GLP-NPs was 288.4 nm and PDI was 0.340.					
33933541	7	7	theme	antibacterial	1077:1089	arg1	activities					1091:1100	antibacterial activities	1077:1100	antibacterial activities	1077:1100	GLP-NPs-AgNPs had better antioxidant and antibacterial activities than GLP and GLP-NPs.					
33933541	3	8	theme	GLP-NPs	586:592	arg1	nm					604:605	288.4 nm	598:605	288.4 nm	598:605	Then, GLP-NPs were prepared by nano-precipitation method, the particle size of GLP-NPs was 288.4 nm and PDI was 0.340.					
33933541	3	8	theme	GLP-NPs	586:592	arg1	size					578:581	the particle size	565:581	the particle size of GLP-NPs	565:592	Then, GLP-NPs were prepared by nano-precipitation method, the particle size of GLP-NPs was 288.4 nm and PDI was 0.340.					
33933541	5	9	theme	X-ray	863:867	arg1	diffraction					869:879	X-ray diffraction	863:879	X-ray diffraction (XRD)	863:885	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	5	9	theme	X-ray	863:867	arg1	XRD					882:884	XRD	882:884	XRD	882:884	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	8	10	theme	nanoparticles	1208:1220	arg1	synthesis					1188:1196	the synthesis	1184:1196	the synthesis of silver nanoparticles (AgNPs)	1184:1228	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	2	11	theme	terminal	438:445	arg1	-α-D-Glcp					459:467	1→)-α-D-Glcp	456:467	1→)-α-D-Glcp	456:467	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	11	theme	terminal	438:445	arg1	group					447:451	the terminal group	434:451	the terminal group of 1→)-α-D-Glcp	434:467	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	12	dep	-α-D-Glcp	459:467	arg1	1→					456:457	1→	456:457	1→	456:457	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	6	13	with	rate	975:978	arg1	polysaccharides					1009:1023	polysaccharides	1009:1023	polysaccharides	1009:1023	The yield of GLP-NPs-AgNPs was 38.77%, the particle size was 12.5 nm and the chelation rate of silver nanoparticles with polysaccharides was 67.5%.					
33933541	6	14	theme	silver	983:988	arg1	nanoparticles					990:1002	silver nanoparticles	983:1002	silver nanoparticles	983:1002	The yield of GLP-NPs-AgNPs was 38.77%, the particle size was 12.5 nm and the chelation rate of silver nanoparticles with polysaccharides was 67.5%.					
33933541	7	15	theme	better	1054:1059	arg1	antioxidant					1061:1071	better antioxidant	1054:1071	better antioxidant	1054:1071	GLP-NPs-AgNPs had better antioxidant and antibacterial activities than GLP and GLP-NPs.					
33933541	0	16	theme	Glehnia	85:91	arg1	littoralis					93:102	Glehnia littoralis	85:102	Glehnia littoralis	85:102	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	8	17	theme	G.	1236:1237	arg1	GLP-NPs					1281:1287	GLP-NPs	1281:1287	GLP-NPs	1281:1287	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	8	17	theme	G.	1236:1237	arg1	nanoparticles					1266:1278	G. littoralis polysaccharides nanoparticles	1236:1278	G. littoralis polysaccharides nanoparticles (GLP-NPs)	1236:1288	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	8	18	theme	present	1131:1137	arg1	work					1139:1142	the present work	1127:1142	the present work	1127:1142	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	0	19	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	0	20	theme	biological	32:41	arg1	activities					43:52	biological activities	32:52	biological activities	32:52	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	1	21	theme	G.	256:257	arg1	littoralis					259:268	G. littoralis	256:268	G. littoralis	256:268	A novel polysaccharide (GLP) with a molecular weight of 1.37 × 105 Da was purified from the roots of G. littoralis.					
33933541	7	22	contain	had	1050:1052	arg1	GLP-NPs-AgNPs					1036:1048	GLP-NPs-AgNPs	1036:1048	GLP-NPs-AgNPs	1036:1048	GLP-NPs-AgNPs had better antioxidant and antibacterial activities than GLP and GLP-NPs.					
33933541	7	22	contain	had	1050:1052	arg2	activities					1091:1100	antibacterial activities	1077:1100	antibacterial activities	1077:1100	GLP-NPs-AgNPs had better antioxidant and antibacterial activities than GLP and GLP-NPs.					
33933541	7	22	contain	had	1050:1052	arg2	antioxidant					1061:1071	better antioxidant	1054:1071	better antioxidant	1054:1071	GLP-NPs-AgNPs had better antioxidant and antibacterial activities than GLP and GLP-NPs.					
33933541	2	23	theme	monosaccharide	277:290	arg1	1D					334:335	1D	334:335	1D	334:335	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	23	theme	monosaccharide	277:290	arg1	NMR					344:346	2D NMR	341:346	2D NMR	341:346	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	23	theme	monosaccharide	277:290	arg1	GC-MS					327:331	GC-MS	327:331	GC-MS	327:331	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	23	theme	monosaccharide	277:290	arg1	composition					292:302	monosaccharide composition	277:302	monosaccharide composition	277:302	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	23	theme	monosaccharide	277:290	arg1	analysis					317:324	methylation analysis	305:324	methylation analysis	305:324	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	1	24	theme	molecular	191:199	arg1	weight					201:206	a molecular weight	189:206	a molecular weight of 1.37 × 105 Da	189:223	A novel polysaccharide (GLP) with a molecular weight of 1.37 × 105 Da was purified from the roots of G. littoralis.					
33933541	5	25	theme	ultraviolet-visible	723:741	arg1	Fourier					771:777	Fourier	771:777	Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD)	771:885	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	5	25	theme	ultraviolet-visible	723:741	arg1	spectrophotometer					743:759	ultraviolet-visible spectrophotometer	723:759	ultraviolet-visible spectrophotometer (UV-Vis)	723:768	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	1	26	theme	littoralis	259:268	arg1	roots					247:251	the roots	243:251	the roots of G. littoralis	243:268	A novel polysaccharide (GLP) with a molecular weight of 1.37 × 105 Da was purified from the roots of G. littoralis.					
33933541	2	27	theme	-α-D-Glcp	459:467	arg1	-α-D-Glcp					459:467	1→)-α-D-Glcp	456:467	1→)-α-D-Glcp	456:467	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	27	theme	-α-D-Glcp	459:467	arg1	group					447:451	the terminal group	434:451	the terminal group of 1→)-α-D-Glcp	434:467	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	0	28	from	littoralis	93:102	arg1	polysaccharide					65:78	a novel polysaccharide	57:78	a novel polysaccharide from Glehnia littoralis	57:102	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	0	28	from	littoralis	93:102	arg1	application					112:122	its application	108:122	its application in preparation of nano-silver	108:152	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	0	28	from	littoralis	93:102	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	0	28	from	littoralis	93:102	arg1	activities					43:52	biological activities	32:52	biological activities	32:52	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	8	29	theme	littoralis	1239:1248	arg1	GLP-NPs					1281:1287	GLP-NPs	1281:1287	GLP-NPs	1281:1287	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	8	29	theme	littoralis	1239:1248	arg1	nanoparticles					1266:1278	G. littoralis polysaccharides nanoparticles	1236:1278	G. littoralis polysaccharides nanoparticles (GLP-NPs)	1236:1288	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	2	30	theme	-α-D-Glcp	399:407	arg1	structure					353:361	the structure	349:361	the structure of GLP	349:368	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	30	theme	-α-D-Glcp	399:407	arg1	linkage					419:425	a 1 → 4)-α-D-Glcp glycoside linkage	391:425	a 1 → 4)-α-D-Glcp glycoside linkage	391:425	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	6	31	theme	nanoparticles	990:1002	arg1	%					1033:1033	67.5%	1029:1033	67.5%	1029:1033	The yield of GLP-NPs-AgNPs was 38.77%, the particle size was 12.5 nm and the chelation rate of silver nanoparticles with polysaccharides was 67.5%.					
33933541	6	31	theme	nanoparticles	990:1002	arg1	rate					975:978	the chelation rate	961:978	the chelation rate of silver nanoparticles with polysaccharides	961:1023	The yield of GLP-NPs-AgNPs was 38.77%, the particle size was 12.5 nm and the chelation rate of silver nanoparticles with polysaccharides was 67.5%.					
33933541	8	32	theme	simple	1147:1152	arg1	approach					1171:1178	a simple and eco-friendly approach	1145:1178	a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs)	1145:1228	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	5	33	dep	transform	779:787	arg1	infrared					789:796	infrared	789:796	transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD)	779:885	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	2	34	theme	GLP	366:368	arg1	structure					353:361	the structure	349:361	the structure of GLP	349:368	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	34	theme	GLP	366:368	arg1	linkage					419:425	a 1 → 4)-α-D-Glcp glycoside linkage	391:425	a 1 → 4)-α-D-Glcp glycoside linkage	391:425	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	0	35	theme	nano-silver	142:152	arg1	preparation					127:137	preparation	127:137	preparation of nano-silver	127:152	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	6	36	theme	GLP-NPs-AgNPs	901:913	arg1	%					924:924	38.77%	919:924	38.77%	919:924	The yield of GLP-NPs-AgNPs was 38.77%, the particle size was 12.5 nm and the chelation rate of silver nanoparticles with polysaccharides was 67.5%.					
33933541	6	36	theme	GLP-NPs-AgNPs	901:913	arg1	yield					892:896	The yield	888:896	The yield of GLP-NPs-AgNPs	888:913	The yield of GLP-NPs-AgNPs was 38.77%, the particle size was 12.5 nm and the chelation rate of silver nanoparticles with polysaccharides was 67.5%.					
33933541	1	37	theme	1.37	211:214	arg1	×					216:216	×	216:216	×	216:216	A novel polysaccharide (GLP) with a molecular weight of 1.37 × 105 Da was purified from the roots of G. littoralis.					
33933541	0	38	theme	novel	59:63	arg1	polysaccharide					65:78	a novel polysaccharide	57:78	a novel polysaccharide from Glehnia littoralis	57:102	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	2	39	theme	main	487:490	arg1	chain					492:496	the main chain	483:496	the main chain	483:496	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	8	40	theme	eco-friendly	1158:1169	arg1	approach					1171:1178	a simple and eco-friendly approach	1145:1178	a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs)	1145:1228	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	2	41	theme	→	395:395	arg1	structure					353:361	the structure	349:361	the structure of GLP	349:368	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	41	theme	→	395:395	arg1	linkage					419:425	a 1 → 4)-α-D-Glcp glycoside linkage	391:425	a 1 → 4)-α-D-Glcp glycoside linkage	391:425	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	3	42	theme	particle	569:576	arg1	nm					604:605	288.4 nm	598:605	288.4 nm	598:605	Then, GLP-NPs were prepared by nano-precipitation method, the particle size of GLP-NPs was 288.4 nm and PDI was 0.340.					
33933541	3	42	theme	particle	569:576	arg1	size					578:581	the particle size	565:581	the particle size of GLP-NPs	565:592	Then, GLP-NPs were prepared by nano-precipitation method, the particle size of GLP-NPs was 288.4 nm and PDI was 0.340.					
33933541	5	43	dep	Fourier	771:777	arg1	transform					779:787	transform	779:787	transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD)	779:885	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	5	44	theme	transmission	820:831	arg1	TEM					854:856	TEM	854:856	TEM	854:856	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	5	44	theme	transmission	820:831	arg1	microscope					842:851	transmission electron microscope	820:851	transmission electron microscope (TEM)	820:857	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	0	45	from	application	112:122	arg1	preparation					127:137	preparation	127:137	preparation of nano-silver	127:152	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	0	45	from	application	112:122	arg1	littoralis					93:102	Glehnia littoralis	85:102	Glehnia littoralis	85:102	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	8	46	theme	reducing	1293:1300	arg1	agent					1302:1306	reducing agent	1293:1306	reducing agent	1293:1306	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	5	47	theme	electron	833:840	arg1	TEM					854:856	TEM	854:856	TEM	854:856	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	5	47	theme	electron	833:840	arg1	microscope					842:851	transmission electron microscope	820:851	transmission electron microscope (TEM)	820:857	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	8	48	theme	polysaccharides	1250:1264	arg1	GLP-NPs					1281:1287	GLP-NPs	1281:1287	GLP-NPs	1281:1287	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	8	48	theme	polysaccharides	1250:1264	arg1	nanoparticles					1266:1278	G. littoralis polysaccharides nanoparticles	1236:1278	G. littoralis polysaccharides nanoparticles (GLP-NPs)	1236:1288	In the present work, a simple and eco-friendly approach for the synthesis of silver nanoparticles (AgNPs) using G. littoralis polysaccharides nanoparticles (GLP-NPs) as reducing agent.					
33933541	4	49	theme	reducing	671:678	arg1	agent					680:684	reducing agent	671:684	reducing agent	671:684	GLP-NPs-AgNPs were prepared using GLP-NPs as reducing agent.					
33933541	2	50	theme	methylation	305:315	arg1	composition					292:302	monosaccharide composition	277:302	monosaccharide composition	277:302	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	50	theme	methylation	305:315	arg1	analysis					317:324	methylation analysis	305:324	methylation analysis	305:324	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	1	51	theme	105	218:220	arg1	×					216:216	×	216:216	×	216:216	A novel polysaccharide (GLP) with a molecular weight of 1.37 × 105 Da was purified from the roots of G. littoralis.					
33933541	6	52	theme	chelation	965:973	arg1	%					1033:1033	67.5%	1029:1033	67.5%	1029:1033	The yield of GLP-NPs-AgNPs was 38.77%, the particle size was 12.5 nm and the chelation rate of silver nanoparticles with polysaccharides was 67.5%.					
33933541	6	52	theme	chelation	965:973	arg1	rate					975:978	the chelation rate	961:978	the chelation rate of silver nanoparticles with polysaccharides	961:1023	The yield of GLP-NPs-AgNPs was 38.77%, the particle size was 12.5 nm and the chelation rate of silver nanoparticles with polysaccharides was 67.5%.					
33933541	5	53	dep	spectrophotometer	743:759	arg1	UV-Vis					762:767	UV-Vis	762:767	UV-Vis	762:767	GLP-NPs-AgNPs were characterized by ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM) and X-ray diffraction (XRD).					
33933541	6	54	theme	particle	931:938	arg1	size					940:943	the particle size	927:943	the particle size	927:943	The yield of GLP-NPs-AgNPs was 38.77%, the particle size was 12.5 nm and the chelation rate of silver nanoparticles with polysaccharides was 67.5%.					
33933541	6	54	theme	particle	931:938	arg1	nm					954:955	12.5 nm	949:955	12.5 nm	949:955	The yield of GLP-NPs-AgNPs was 38.77%, the particle size was 12.5 nm and the chelation rate of silver nanoparticles with polysaccharides was 67.5%.					
33933541	2	55	theme	2D	341:342	arg1	NMR					344:346	2D NMR	341:346	2D NMR	341:346	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	2	55	theme	2D	341:342	arg1	composition					292:302	monosaccharide composition	277:302	monosaccharide composition	277:302	Using monosaccharide composition, methylation analysis, GC-MS, 1D and 2D NMR, the structure of GLP was determined to be a 1 → 4)-α-D-Glcp glycoside linkage, while the terminal group of 1→)-α-D-Glcp was bonded to the main chain via O-6.					
33933541	1	56	theme	Da	222:223	arg1	weight					201:206	a molecular weight	189:206	a molecular weight of 1.37 × 105 Da	189:223	A novel polysaccharide (GLP) with a molecular weight of 1.37 × 105 Da was purified from the roots of G. littoralis.					
33933541	0	57	theme	polysaccharide	65:78	arg1	application					112:122	its application	108:122	its application in preparation of nano-silver	108:152	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	0	57	theme	polysaccharide	65:78	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	0	57	theme	polysaccharide	65:78	arg1	activities					43:52	biological activities	32:52	biological activities	32:52	Structural characterization and biological activities of a novel polysaccharide from Glehnia littoralis and its application in preparation of nano-silver.					
33933541	1	58	theme	novel	157:161	arg1	GLP					179:181	GLP	179:181	GLP	179:181	A novel polysaccharide (GLP) with a molecular weight of 1.37 × 105 Da was purified from the roots of G. littoralis.					
33933541	1	58	theme	novel	157:161	arg1	polysaccharide					163:176	A novel polysaccharide	155:176	A novel polysaccharide (GLP) with a molecular weight of 1.37 × 105 Da	155:223	A novel polysaccharide (GLP) with a molecular weight of 1.37 × 105 Da was purified from the roots of G. littoralis.					
32061702	8	0	theme	lowest	1526:1531	arg1	solubility					1533:1542	the lowest solubility	1522:1542	barrier as well as the lowest solubility	1503:1542	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	2	1	dep	glycerol	280:287	arg1	the					276:278	the	276:278	the	276:278	lactuca at different extraction conditions, by varying the glycerol or sorbitol in specific plasticizer concentration.					
32061702	4	2	theme	extraction	484:493	arg1	procedure					495:503	the extraction procedure	480:503	the extraction procedure	480:503	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	4	3	theme	stimuli-responsive	601:618	arg1	behavior					692:699	stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior	601:699	stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior	601:699	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	0	4	theme	condition	70:78	arg1	stimuli					32:38	stimuli	32:38	stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties	32:142	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	3	5	theme	molecular	365:373	arg1	weight					375:380	molecular weight	365:380	molecular weight	365:380	Chemical composition and molecular weight characteristics of ulvans were carried out by GLC and HPSEC analysis, respectively.					
32061702	4	6	theme	chemical	640:647	arg1	composition					649:659	its peculiar chemical composition	627:659	its peculiar chemical composition	627:659	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	5	7	theme	extraction	895:904	arg1	conditions					906:915	extraction conditions	895:915	extraction conditions	895:915	When using the terminology of stimuli-responsive system, we declare that the physico-chemical, barrier, optical, and mechanical characteristics of ulvan films system are strongly responsive to extraction conditions and to plasticizer type and concentration.					
32061702	0	8	theme	physico-chemical	83:98	arg1	barrier					110:116	physico-chemical, optical, barrier and mechanical properties	83:142	barrier	110:116	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	0	9	from	plasticizer	43:53	arg1	properties					133:142	physico-chemical, optical, barrier and mechanical properties	83:142	properties	133:142	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	6	10	theme	water	1034:1038	arg1	solubility					1040:1049	water solubility	1034:1049	water solubility	1034:1049	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	4	11	theme	due	620:622	arg1	behavior					692:699	stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior	601:699	stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior	601:699	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	3	12	theme	composition	349:359	arg1	characteristics					382:396	Chemical composition and molecular weight characteristics	340:396	Chemical composition and molecular weight characteristics of ulvans	340:406	Chemical composition and molecular weight characteristics of ulvans were carried out by GLC and HPSEC analysis, respectively.					
32061702	5	13	theme	films	855:859	arg1	system					861:866	ulvan films system	849:866	ulvan films system	849:866	When using the terminology of stimuli-responsive system, we declare that the physico-chemical, barrier, optical, and mechanical characteristics of ulvan films system are strongly responsive to extraction conditions and to plasticizer type and concentration.					
32061702	9	14	theme	FTIR	1549:1552	arg1	spectra					1554:1560	The FTIR spectra	1545:1560	The FTIR spectra	1545:1560	The FTIR spectra evidenced the expected outcome of the chemical interaction and miscibility kind between polysaccharides and plasticizers.					
32061702	7	15	theme	mechanical	1330:1339	arg1	properties					1341:1350	mechanical properties	1330:1350	mechanical properties	1330:1350	The films prepared with glycerol exhibited better solubility, transparency and mechanical properties, than those with sorbitol.					
32061702	0	16	theme	optical	101:107	arg1	properties					133:142	physico-chemical, optical, barrier and mechanical properties	83:142	properties	133:142	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	0	17	from	stimuli	32:38	arg1	properties					133:142	physico-chemical, optical, barrier and mechanical properties	83:142	properties	133:142	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	6	18	theme	water	1052:1056	arg1	permeability					1064:1075	water vapor permeability	1052:1075	water vapor permeability (WVP)	1052:1081	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	18	theme	water	1052:1056	arg1	WVP					1078:1080	WVP	1078:1080	WVP	1078:1080	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	7	19	dep	better	1294:1299	arg1	transparency					1313:1324	transparency	1313:1324	transparency	1313:1324	The films prepared with glycerol exhibited better solubility, transparency and mechanical properties, than those with sorbitol.					
32061702	7	19	dep	better	1294:1299	arg1	properties					1341:1350	mechanical properties	1330:1350	mechanical properties	1330:1350	The films prepared with glycerol exhibited better solubility, transparency and mechanical properties, than those with sorbitol.					
32061702	7	19	dep	better	1294:1299	arg1	solubility					1301:1310	solubility	1301:1310	solubility	1301:1310	The films prepared with glycerol exhibited better solubility, transparency and mechanical properties, than those with sorbitol.					
32061702	4	20	theme	peculiar	631:638	arg1	composition					649:659	its peculiar chemical composition	627:659	its peculiar chemical composition	627:659	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	9	21	dep	interaction	1609:1619	arg1	the					1596:1598	the	1596:1598	the	1596:1598	The FTIR spectra evidenced the expected outcome of the chemical interaction and miscibility kind between polysaccharides and plasticizers.					
32061702	3	22	theme	ulvans	401:406	arg1	characteristics					382:396	Chemical composition and molecular weight characteristics	340:396	Chemical composition and molecular weight characteristics of ulvans	340:406	Chemical composition and molecular weight characteristics of ulvans were carried out by GLC and HPSEC analysis, respectively.					
32061702	6	23	theme	films	1174:1178	arg1	transparency					1084:1095	transparency	1084:1095	transparency	1084:1095	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	23	theme	films	1174:1178	arg1	MC					1029:1030	MC	1029:1030	MC	1029:1030	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	23	theme	films	1174:1178	arg1	thickness					1000:1008	thickness	1000:1008	thickness	1000:1008	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	23	theme	films	1174:1178	arg1	permeability					1064:1075	water vapor permeability	1052:1075	water vapor permeability (WVP)	1052:1081	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	23	theme	films	1174:1178	arg1	protection					1154:1163	visible light protection	1140:1163	visible light protection	1140:1163	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	23	theme	films	1174:1178	arg1	elongation					1098:1107	elongation	1098:1107	elongation at break (EAB)	1098:1122	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	23	theme	films	1174:1178	arg1	WVP					1078:1080	WVP	1078:1080	WVP	1078:1080	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	23	theme	films	1174:1178	arg1	content					1020:1026	moisture content	1011:1026	moisture content (MC)	1011:1031	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	23	theme	films	1174:1178	arg1	solubility					1040:1049	water solubility	1034:1049	water solubility	1034:1049	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	2	24	theme	specific	304:311	arg1	concentration					325:337	specific plasticizer concentration	304:337	specific plasticizer concentration	304:337	lactuca at different extraction conditions, by varying the glycerol or sorbitol in specific plasticizer concentration.					
32061702	5	25	theme	plasticizer	924:934	arg1	type					936:939	plasticizer type	924:939	plasticizer type	924:939	When using the terminology of stimuli-responsive system, we declare that the physico-chemical, barrier, optical, and mechanical characteristics of ulvan films system are strongly responsive to extraction conditions and to plasticizer type and concentration.					
32061702	5	26	theme	system	861:866	arg1	responsive					881:890	responsive	881:890	responsive	881:890	When using the terminology of stimuli-responsive system, we declare that the physico-chemical, barrier, optical, and mechanical characteristics of ulvan films system are strongly responsive to extraction conditions and to plasticizer type and concentration.					
32061702	5	26	theme	system	861:866	arg1	physico-chemical					779:794	physico-chemical	779:794	physico-chemical	779:794	When using the terminology of stimuli-responsive system, we declare that the physico-chemical, barrier, optical, and mechanical characteristics of ulvan films system are strongly responsive to extraction conditions and to plasticizer type and concentration.					
32061702	6	27	theme	tensile	1204:1210	arg1	strength					1212:1219	their tensile strength	1198:1219	their tensile strength (TS)	1198:1224	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	27	theme	tensile	1204:1210	arg1	TS					1222:1223	TS	1222:1223	TS	1222:1223	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	28	theme	ulvan	1168:1172	arg1	films					1174:1178	ulvan films	1168:1178	ulvan films	1168:1178	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	3	29	theme	HPSEC	436:440	arg1	analysis					442:449	HPSEC analysis	436:449	HPSEC analysis	436:449	Chemical composition and molecular weight characteristics of ulvans were carried out by GLC and HPSEC analysis, respectively.					
32061702	0	30	theme	responsive	18:27	arg1	films					12:16	films	12:16	films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties	12:142	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	4	31	theme	reproducible	562:573	arg1	ulvans					538:543	ulvans	538:543	ulvans	538:543	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	4	31	theme	reproducible	562:573	arg1	materials					588:596	reproducible and suitable materials	562:596	reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior	562:699	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	6	32	from	break	1112:1116	arg1	transparency					1084:1095	transparency	1084:1095	transparency	1084:1095	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	32	from	break	1112:1116	arg1	MC					1029:1030	MC	1029:1030	MC	1029:1030	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	32	from	break	1112:1116	arg1	thickness					1000:1008	thickness	1000:1008	thickness	1000:1008	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	32	from	break	1112:1116	arg1	permeability					1064:1075	water vapor permeability	1052:1075	water vapor permeability (WVP)	1052:1081	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	32	from	break	1112:1116	arg1	protection					1154:1163	visible light protection	1140:1163	visible light protection	1140:1163	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	32	from	break	1112:1116	arg1	elongation					1098:1107	elongation	1098:1107	elongation at break (EAB)	1098:1122	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	32	from	break	1112:1116	arg1	WVP					1078:1080	WVP	1078:1080	WVP	1078:1080	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	32	from	break	1112:1116	arg1	content					1020:1026	moisture content	1011:1026	moisture content (MC)	1011:1031	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	32	from	break	1112:1116	arg1	solubility					1040:1049	water solubility	1034:1049	water solubility	1034:1049	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	9	33	theme	expected	1576:1583	arg1	outcome					1585:1591	the expected outcome	1572:1591	the expected outcome of the chemical interaction and miscibility kind between polysaccharides and plasticizers	1572:1681	The FTIR spectra evidenced the expected outcome of the chemical interaction and miscibility kind between polysaccharides and plasticizers.					
32061702	0	34	theme	barrier	110:116	arg1	properties					133:142	physico-chemical, optical, barrier and mechanical properties	83:142	properties	133:142	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	6	35	dep	increased	1180:1188	arg1	while					1191:1195	while	1191:1195	while	1191:1195	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	5	36	theme	mechanical	819:828	arg1	characteristics					830:844	mechanical characteristics	819:844	mechanical characteristics	819:844	When using the terminology of stimuli-responsive system, we declare that the physico-chemical, barrier, optical, and mechanical characteristics of ulvan films system are strongly responsive to extraction conditions and to plasticizer type and concentration.					
32061702	4	37	theme	suitable	579:586	arg1	ulvans					538:543	ulvans	538:543	ulvans	538:543	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	4	37	theme	suitable	579:586	arg1	materials					588:596	reproducible and suitable materials	562:596	reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior	562:699	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	0	38	from	condition	70:78	arg1	properties					133:142	physico-chemical, optical, barrier and mechanical properties	83:142	properties	133:142	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	4	39	theme	behavior	692:699	arg1	ulvans					538:543	ulvans	538:543	ulvans	538:543	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	4	39	theme	behavior	692:699	arg1	materials					588:596	reproducible and suitable materials	562:596	reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior	562:699	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	8	40	contain	had	1419:1421	arg2	resistance					1445:1454	moisture resistance	1436:1454	moisture resistance	1436:1454	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	8	40	contain	had	1419:1421	arg2	light					1497:1501	visible and UV light barrier as well as the lowest solubility	1482:1542	light	1497:1501	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	8	40	contain	had	1419:1421	arg2	vapor					1475:1479	more effective vapor	1460:1479	more effective vapor	1460:1479	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	8	40	contain	had	1419:1421	arg1	films					1392:1396	the films	1388:1396	the films formed with sorbitol	1388:1417	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	0	41	theme	mechanical	122:131	arg1	properties					133:142	physico-chemical, optical, barrier and mechanical properties	83:142	properties	133:142	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	6	42	theme	vapor	1058:1062	arg1	permeability					1064:1075	water vapor permeability	1052:1075	water vapor permeability (WVP)	1052:1081	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	42	theme	vapor	1058:1062	arg1	WVP					1078:1080	WVP	1078:1080	WVP	1078:1080	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	9	43	theme	kind	1637:1640	arg1	outcome					1585:1591	the expected outcome	1572:1591	the expected outcome of the chemical interaction and miscibility kind between polysaccharides and plasticizers	1572:1681	The FTIR spectra evidenced the expected outcome of the chemical interaction and miscibility kind between polysaccharides and plasticizers.					
32061702	4	44	theme	molecular	682:690	arg1	behavior					692:699	stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior	601:699	stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior	601:699	Regardless of the extraction procedure, the investigation revealed that ulvans were selected as reproducible and suitable materials of stimuli-responsive due to its peculiar chemical composition and self-aggregation molecular behavior.					
32061702	0	45	theme	plasticizer	43:53	arg1	stimuli					32:38	stimuli	32:38	stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties	32:142	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	6	46	theme	moisture	1011:1018	arg1	MC					1029:1030	MC	1029:1030	MC	1029:1030	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	46	theme	moisture	1011:1018	arg1	content					1020:1026	moisture content	1011:1026	moisture content (MC)	1011:1031	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	6	47	dep	thickness	1000:1008	arg1	UV					1133:1134	UV	1133:1134	UV	1133:1134	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	8	48	theme	effective	1465:1473	arg1	light					1497:1501	visible and UV light barrier as well as the lowest solubility	1482:1542	light	1497:1501	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	8	48	theme	effective	1465:1473	arg1	vapor					1475:1479	more effective vapor	1460:1479	more effective vapor	1460:1479	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	9	49	theme	chemical	1600:1607	arg1	interaction					1609:1619	chemical interaction	1600:1619	chemical interaction	1600:1619	The FTIR spectra evidenced the expected outcome of the chemical interaction and miscibility kind between polysaccharides and plasticizers.					
32061702	6	50	theme	light	1148:1152	arg1	protection					1154:1163	visible light protection	1140:1163	visible light protection	1140:1163	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	5	51	theme	ulvan	849:853	arg1	system					861:866	ulvan films system	849:866	ulvan films system	849:866	When using the terminology of stimuli-responsive system, we declare that the physico-chemical, barrier, optical, and mechanical characteristics of ulvan films system are strongly responsive to extraction conditions and to plasticizer type and concentration.					
32061702	8	52	theme	visible	1482:1488	arg1	light					1497:1501	visible and UV light barrier as well as the lowest solubility	1482:1542	light	1497:1501	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	8	52	theme	visible	1482:1488	arg1	vapor					1475:1479	more effective vapor	1460:1479	more effective vapor	1460:1479	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	9	53	theme	interaction	1609:1619	arg1	outcome					1585:1591	the expected outcome	1572:1591	the expected outcome of the chemical interaction and miscibility kind between polysaccharides and plasticizers	1572:1681	The FTIR spectra evidenced the expected outcome of the chemical interaction and miscibility kind between polysaccharides and plasticizers.					
32061702	6	54	theme	visible	1140:1146	arg1	protection					1154:1163	visible light protection	1140:1163	visible light protection	1140:1163	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	8	55	theme	moisture	1436:1443	arg1	resistance					1445:1454	moisture resistance	1436:1454	moisture resistance	1436:1454	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	5	56	theme	stimuli-responsive	732:749	arg1	system					751:756	stimuli-responsive system	732:756	stimuli-responsive system	732:756	When using the terminology of stimuli-responsive system, we declare that the physico-chemical, barrier, optical, and mechanical characteristics of ulvan films system are strongly responsive to extraction conditions and to plasticizer type and concentration.					
32061702	8	57	dep	light	1497:1501	arg1	barrier					1503:1509	barrier	1503:1509	barrier as well as the lowest solubility	1503:1542	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	8	57	dep	light	1497:1501	arg1	solubility					1533:1542	the lowest solubility	1522:1542	barrier as well as the lowest solubility	1503:1542	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	8	58	theme	greatest	1427:1434	arg1	resistance					1445:1454	moisture resistance	1436:1454	moisture resistance	1436:1454	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	0	59	from	properties	133:142	arg1	stimuli					32:38	stimuli	32:38	stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties	32:142	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	5	60	theme	system	751:756	arg1	terminology					717:727	the terminology	713:727	the terminology of stimuli-responsive system	713:756	When using the terminology of stimuli-responsive system, we declare that the physico-chemical, barrier, optical, and mechanical characteristics of ulvan films system are strongly responsive to extraction conditions and to plasticizer type and concentration.					
32061702	8	61	theme	UV	1494:1495	arg1	light					1497:1501	visible and UV light barrier as well as the lowest solubility	1482:1542	light	1497:1501	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	8	61	theme	UV	1494:1495	arg1	vapor					1475:1479	more effective vapor	1460:1479	more effective vapor	1460:1479	However, the films formed with sorbitol had the greatest moisture resistance and more effective vapor, visible and UV light barrier as well as the lowest solubility.					
32061702	9	62	theme	miscibility	1625:1635	arg1	kind					1637:1640	miscibility kind	1625:1640	miscibility kind	1625:1640	The FTIR spectra evidenced the expected outcome of the chemical interaction and miscibility kind between polysaccharides and plasticizers.					
32061702	2	63	theme	plasticizer	313:323	arg1	concentration					325:337	specific plasticizer concentration	304:337	specific plasticizer concentration	304:337	lactuca at different extraction conditions, by varying the glycerol or sorbitol in specific plasticizer concentration.					
32061702	2	64	from	glycerol	280:287	arg1	concentration					325:337	specific plasticizer concentration	304:337	specific plasticizer concentration	304:337	lactuca at different extraction conditions, by varying the glycerol or sorbitol in specific plasticizer concentration.					
32061702	0	65	theme	extraction	59:68	arg1	condition					70:78	extraction condition	59:78	extraction condition	59:78	Smart ulvan films responsive to stimuli of plasticizer and extraction condition in physico-chemical, optical, barrier and mechanical properties.					
32061702	2	66	theme	extraction	242:251	arg1	conditions					253:262	different extraction conditions	232:262	different extraction conditions	232:262	lactuca at different extraction conditions, by varying the glycerol or sorbitol in specific plasticizer concentration.					
32061702	6	67	theme	plasticizer	963:973	arg1	concentration					975:987	plasticizer concentration	963:987	plasticizer concentration	963:987	As plasticizer concentration increased, thickness, moisture content (MC), water solubility, water vapor permeability (WVP), transparency, elongation at break (EAB), usually UV and visible light protection of ulvan films increased, while, their tensile strength (TS) decreased significantly.					
32061702	3	68	theme	Chemical	340:347	arg1	composition					349:359	Chemical composition	340:359	Chemical composition	340:359	Chemical composition and molecular weight characteristics of ulvans were carried out by GLC and HPSEC analysis, respectively.					
32061702	2	69	theme	different	232:240	arg1	conditions					253:262	different extraction conditions	232:262	different extraction conditions	232:262	lactuca at different extraction conditions, by varying the glycerol or sorbitol in specific plasticizer concentration.					
32061702	1	70	theme	Smart	145:149	arg1	films					151:155	Smart films	145:155	Smart films	145:155	Smart films were produced with ulvan extracted from the green seaweed Ulva.					
32061702	2	71	from	sorbitol	292:299	arg1	concentration					325:337	specific plasticizer concentration	304:337	specific plasticizer concentration	304:337	lactuca at different extraction conditions, by varying the glycerol or sorbitol in specific plasticizer concentration.					
32061702	3	72	theme	weight	375:380	arg1	characteristics					382:396	Chemical composition and molecular weight characteristics	340:396	Chemical composition and molecular weight characteristics of ulvans	340:406	Chemical composition and molecular weight characteristics of ulvans were carried out by GLC and HPSEC analysis, respectively.					
32926901	6	0	theme	Phascolarctobacterium	964:984	arg1	abundance					912:920	the relative abundance	899:920	the relative abundance of Coprococcus, Adlercreutzia, Roseburia, Phascolarctobacterium	899:984	While CSP-1 increased the relative abundance of Coprococcus, Adlercreutzia, Roseburia, Phascolarctobacterium, and decreased the relative abundance of Bacteroides, Ruminococcus and Oscillospira.					
32926901	5	1	theme	genus	688:692	arg1	level					694:698	the genus level	684:698	the genus level	684:698	At the genus level, GPOP-1 increased the relative abundance of Bacteroides, Phascolarctobacterium, and decreased the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12.					
32926901	9	2	theme	fatty	1439:1443	arg1	acids					1445:1449	total short chain fatty acids	1421:1449	total short chain fatty acids	1421:1449	Furthermore, both GPOP-1 and CSP-1 significantly increased thymus and spleen indexes and total short chain fatty acids production in mice.					
32926901	6	3	theme	Ruminococcus	1040:1051	arg1	abundance					1014:1022	the relative abundance	1001:1022	the relative abundance of Bacteroides, Ruminococcus and Oscillospira	1001:1068	While CSP-1 increased the relative abundance of Coprococcus, Adlercreutzia, Roseburia, Phascolarctobacterium, and decreased the relative abundance of Bacteroides, Ruminococcus and Oscillospira.					
32926901	4	4	theme	microbiota	622:631	arg1	composition					599:609	the composition	595:609	the composition of the gut microbiota	595:631	The results showed that GPOP-1 and CSP-1 altered the composition of the gut microbiota and promoted the growth of beneficial bacteria.					
32926901	0	5	theme	prebiotic	82:90	arg1	functions					92:100	prebiotic functions	82:100	prebiotic functions	82:100	Marine polysaccharides from Gelidium pacificum Okamura and Cereus sinensis reveal prebiotic functions.					
32926901	7	6	theme	immune	1133:1138	arg1	index					1146:1150	immune organ index	1133:1150	immune organ index	1133:1150	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	2	7	link	plant-derived	265:277	arg1	GPOP-1					322:327	GPOP-1	322:327	GPOP-1	322:327	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	7	link	plant-derived	265:277	arg1	polysaccharide					306:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide	249:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1)	249:328	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	7	8	from	weight	1125:1130	arg1	mice					1208:1211	normal mice	1201:1211	normal mice	1201:1211	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	6	9	theme	Bacteroides	1027:1037	arg1	abundance					1014:1022	the relative abundance	1001:1022	the relative abundance of Bacteroides, Ruminococcus and Oscillospira	1001:1068	While CSP-1 increased the relative abundance of Coprococcus, Adlercreutzia, Roseburia, Phascolarctobacterium, and decreased the relative abundance of Bacteroides, Ruminococcus and Oscillospira.					
32926901	3	10	theme	high-throughput	518:532	arg1	sequencing					534:543	16S rRNA high-throughput sequencing	509:543	16S rRNA high-throughput sequencing	509:543	The composition and diversity of gut microbiota were evaluated using 16S rRNA high-throughput sequencing.					
32926901	4	11	theme	bacteria	671:678	arg1	growth					650:655	the growth	646:655	the growth of beneficial bacteria	646:678	The results showed that GPOP-1 and CSP-1 altered the composition of the gut microbiota and promoted the growth of beneficial bacteria.					
32926901	2	12	from	effects	238:244	arg1	mice					405:408	normal mice	398:408	normal mice	398:408	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	1	13	theme	gut	183:185	arg1	microbiota					187:196	gut microbiota	183:196	gut microbiota	183:196	Many marine polysaccharides as prebiotics can promote host health by modulating gut microbiota.					
32926901	5	14	theme	Allobaculum	848:858	arg1	abundance					807:815	the relative abundance	794:815	the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12	794:874	At the genus level, GPOP-1 increased the relative abundance of Bacteroides, Phascolarctobacterium, and decreased the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12.					
32926901	0	15	from	Okamura	47:53	arg1	polysaccharides					7:21	Marine polysaccharides	0:21	Marine polysaccharides from Gelidium pacificum Okamura and Cereus sinensis	0:73	Marine polysaccharides from Gelidium pacificum Okamura and Cereus sinensis reveal prebiotic functions.					
32926901	10	16	theme	prebiotic	1508:1516	arg1	effects					1518:1524	prebiotic effects	1508:1524	prebiotic effects	1508:1524	In summary, GPOP-1 and CSP-1 exerted prebiotic effects on normal mice.					
32926901	2	17	theme	polysaccharide	306:319	arg1	effects					238:244	the beneficial effects	223:244	the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice	223:408	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	18	theme	gut	424:426	arg1	microbiota					428:437	gut microbiota	424:437	gut microbiota	424:437	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	8	19	theme	normal	1230:1235	arg1	group					1245:1249	the normal control group	1226:1249	the normal control group	1226:1249	Compared to the normal control group, GPOP-1 decreased average weight gain while CSP-1 increased average weight gain.					
32926901	5	20	theme	Ruminococcus	820:831	arg1	abundance					807:815	the relative abundance	794:815	the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12	794:874	At the genus level, GPOP-1 increased the relative abundance of Bacteroides, Phascolarctobacterium, and decreased the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12.					
32926901	3	21	dep	composition	444:454	arg1	The					440:442	The	440:442	The	440:442	The composition and diversity of gut microbiota were evaluated using 16S rRNA high-throughput sequencing.					
32926901	2	22	theme	Cereus	356:361	arg1	CSP-1					388:392	CSP-1	388:392	CSP-1	388:392	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	22	theme	Cereus	356:361	arg1	polysaccharide					372:385	marine animal-derived Cereus sinensis polysaccharide	334:385	marine animal-derived Cereus sinensis polysaccharide (CSP-1)	334:393	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	0	23	theme	Marine	0:5	arg1	polysaccharides					7:21	Marine polysaccharides	0:21	Marine polysaccharides from Gelidium pacificum Okamura and Cereus sinensis	0:73	Marine polysaccharides from Gelidium pacificum Okamura and Cereus sinensis reveal prebiotic functions.					
32926901	7	24	theme	normal	1201:1206	arg1	mice					1208:1211	normal mice	1201:1211	normal mice	1201:1211	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	7	25	theme	organ	1140:1144	arg1	index					1146:1150	immune organ index	1133:1150	immune organ index	1133:1150	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	1	26	theme	Many	103:106	arg1	polysaccharides					115:129	Many marine polysaccharides	103:129	Many marine polysaccharides as prebiotics	103:143	Many marine polysaccharides as prebiotics can promote host health by modulating gut microbiota.					
32926901	7	27	theme	short-chain	1174:1184	arg1	acids					1192:1196	short-chain fatty acids	1174:1196	short-chain fatty acids	1174:1196	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	5	28	theme	Dorea	861:865	arg1	abundance					807:815	the relative abundance	794:815	the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12	794:874	At the genus level, GPOP-1 increased the relative abundance of Bacteroides, Phascolarctobacterium, and decreased the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12.					
32926901	2	29	theme	marine	334:339	arg1	CSP-1					388:392	CSP-1	388:392	CSP-1	388:392	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	29	theme	marine	334:339	arg1	polysaccharide					372:385	marine animal-derived Cereus sinensis polysaccharide	334:385	marine animal-derived Cereus sinensis polysaccharide (CSP-1)	334:393	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	9	30	theme	short	1427:1431	arg1	acids					1445:1449	total short chain fatty acids	1421:1449	total short chain fatty acids	1421:1449	Furthermore, both GPOP-1 and CSP-1 significantly increased thymus and spleen indexes and total short chain fatty acids production in mice.					
32926901	8	31	theme	average	1311:1317	arg1	gain					1326:1329	average weight gain	1311:1329	average weight gain	1311:1329	Compared to the normal control group, GPOP-1 decreased average weight gain while CSP-1 increased average weight gain.					
32926901	2	32	theme	animal-derived	341:354	arg1	CSP-1					388:392	CSP-1	388:392	CSP-1	388:392	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	32	theme	animal-derived	341:354	arg1	polysaccharide					372:385	marine animal-derived Cereus sinensis polysaccharide	334:385	marine animal-derived Cereus sinensis polysaccharide (CSP-1)	334:393	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	7	33	from	index	1146:1150	arg1	mice					1208:1211	normal mice	1201:1211	normal mice	1201:1211	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	3	34	theme	16S	509:511	arg1	sequencing					534:543	16S rRNA high-throughput sequencing	509:543	16S rRNA high-throughput sequencing	509:543	The composition and diversity of gut microbiota were evaluated using 16S rRNA high-throughput sequencing.					
32926901	7	35	from	changes	1075:1081	arg1	microbiota					1094:1103	the gut microbiota	1086:1103	the gut microbiota	1086:1103	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	9	36	theme	spleen	1402:1407	arg1	indexes					1409:1415	thymus and spleen indexes	1391:1415	thymus and spleen indexes	1391:1415	Furthermore, both GPOP-1 and CSP-1 significantly increased thymus and spleen indexes and total short chain fatty acids production in mice.					
32926901	10	37	theme	normal	1529:1534	arg1	mice					1536:1539	normal mice	1529:1539	normal mice	1529:1539	In summary, GPOP-1 and CSP-1 exerted prebiotic effects on normal mice.					
32926901	2	38	theme	marine	258:263	arg1	GPOP-1					322:327	GPOP-1	322:327	GPOP-1	322:327	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	38	theme	marine	258:263	arg1	polysaccharide					306:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide	249:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1)	249:328	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	8	39	theme	control	1237:1243	arg1	group					1245:1249	the normal control group	1226:1249	the normal control group	1226:1249	Compared to the normal control group, GPOP-1 decreased average weight gain while CSP-1 increased average weight gain.					
32926901	9	40	theme	thymus	1391:1396	arg1	indexes					1409:1415	thymus and spleen indexes	1391:1415	thymus and spleen indexes	1391:1415	Furthermore, both GPOP-1 and CSP-1 significantly increased thymus and spleen indexes and total short chain fatty acids production in mice.					
32926901	3	41	theme	gut	473:475	arg1	microbiota					477:486	gut microbiota	473:486	gut microbiota	473:486	The composition and diversity of gut microbiota were evaluated using 16S rRNA high-throughput sequencing.					
32926901	6	42	theme	relative	1005:1012	arg1	abundance					1014:1022	the relative abundance	1001:1022	the relative abundance of Bacteroides, Ruminococcus and Oscillospira	1001:1068	While CSP-1 increased the relative abundance of Coprococcus, Adlercreutzia, Roseburia, Phascolarctobacterium, and decreased the relative abundance of Bacteroides, Ruminococcus and Oscillospira.					
32926901	4	43	theme	gut	618:620	arg1	microbiota					622:631	the gut microbiota	614:631	the gut microbiota	614:631	The results showed that GPOP-1 and CSP-1 altered the composition of the gut microbiota and promoted the growth of beneficial bacteria.					
32926901	6	44	theme	Oscillospira	1057:1068	arg1	abundance					1014:1022	the relative abundance	1001:1022	the relative abundance of Bacteroides, Ruminococcus and Oscillospira	1001:1068	While CSP-1 increased the relative abundance of Coprococcus, Adlercreutzia, Roseburia, Phascolarctobacterium, and decreased the relative abundance of Bacteroides, Ruminococcus and Oscillospira.					
32926901	2	45	theme	purified	249:256	arg1	GPOP-1					322:327	GPOP-1	322:327	GPOP-1	322:327	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	45	theme	purified	249:256	arg1	polysaccharide					306:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide	249:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1)	249:328	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	8	46	theme	weight	1319:1324	arg1	gain					1326:1329	average weight gain	1311:1329	average weight gain	1311:1329	Compared to the normal control group, GPOP-1 decreased average weight gain while CSP-1 increased average weight gain.					
32926901	7	47	theme	fatty	1186:1190	arg1	acids					1192:1196	short-chain fatty acids	1174:1196	short-chain fatty acids	1174:1196	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	7	48	from	production	1160:1169	arg1	mice					1208:1211	normal mice	1201:1211	normal mice	1201:1211	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	5	49	theme	AF12	871:874	arg1	abundance					807:815	the relative abundance	794:815	the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12	794:874	At the genus level, GPOP-1 increased the relative abundance of Bacteroides, Phascolarctobacterium, and decreased the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12.					
32926901	0	50	from	sinensis	66:73	arg1	polysaccharides					7:21	Marine polysaccharides	0:21	Marine polysaccharides from Gelidium pacificum Okamura and Cereus sinensis	0:73	Marine polysaccharides from Gelidium pacificum Okamura and Cereus sinensis reveal prebiotic functions.					
32926901	7	51	theme	gut	1090:1092	arg1	microbiota					1094:1103	the gut microbiota	1086:1103	the gut microbiota	1086:1103	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	7	52	theme	body	1120:1123	arg1	weight					1125:1130	the body weight	1116:1130	the body weight	1116:1130	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	5	53	theme	Phascolarctobacterium	757:777	arg1	abundance					731:739	the relative abundance	718:739	the relative abundance of Bacteroides, Phascolarctobacterium	718:777	At the genus level, GPOP-1 increased the relative abundance of Bacteroides, Phascolarctobacterium, and decreased the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12.					
32926901	9	54	theme	total	1421:1425	arg1	acids					1445:1449	total short chain fatty acids	1421:1449	total short chain fatty acids	1421:1449	Furthermore, both GPOP-1 and CSP-1 significantly increased thymus and spleen indexes and total short chain fatty acids production in mice.					
32926901	9	55	from	production	1451:1460	arg1	mice					1465:1468	mice	1465:1468	mice	1465:1468	Furthermore, both GPOP-1 and CSP-1 significantly increased thymus and spleen indexes and total short chain fatty acids production in mice.					
32926901	2	56	theme	beneficial	227:236	arg1	effects					238:244	the beneficial effects	223:244	the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice	223:408	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	6	57	theme	relative	903:910	arg1	abundance					912:920	the relative abundance	899:920	the relative abundance of Coprococcus, Adlercreutzia, Roseburia, Phascolarctobacterium	899:984	While CSP-1 increased the relative abundance of Coprococcus, Adlercreutzia, Roseburia, Phascolarctobacterium, and decreased the relative abundance of Bacteroides, Ruminococcus and Oscillospira.					
32926901	2	58	link	animal-derived	341:354	arg1	CSP-1					388:392	CSP-1	388:392	CSP-1	388:392	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	58	link	animal-derived	341:354	arg1	polysaccharide					372:385	marine animal-derived Cereus sinensis polysaccharide	334:385	marine animal-derived Cereus sinensis polysaccharide (CSP-1)	334:393	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	5	59	theme	relative	722:729	arg1	abundance					731:739	the relative abundance	718:739	the relative abundance of Bacteroides, Phascolarctobacterium	718:777	At the genus level, GPOP-1 increased the relative abundance of Bacteroides, Phascolarctobacterium, and decreased the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12.					
32926901	2	60	theme	Okamura	298:304	arg1	GPOP-1					322:327	GPOP-1	322:327	GPOP-1	322:327	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	60	theme	Okamura	298:304	arg1	polysaccharide					306:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide	249:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1)	249:328	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	61	theme	polysaccharide	372:385	arg1	effects					238:244	the beneficial effects	223:244	the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice	223:408	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	1	62	theme	marine	108:113	arg1	polysaccharides					115:129	Many marine polysaccharides	103:129	Many marine polysaccharides as prebiotics	103:143	Many marine polysaccharides as prebiotics can promote host health by modulating gut microbiota.					
32926901	9	63	theme	chain	1433:1437	arg1	acids					1445:1449	total short chain fatty acids	1421:1449	total short chain fatty acids	1421:1449	Furthermore, both GPOP-1 and CSP-1 significantly increased thymus and spleen indexes and total short chain fatty acids production in mice.					
32926901	7	64	theme	acids	1192:1196	arg1	index					1146:1150	immune organ index	1133:1150	immune organ index	1133:1150	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	7	64	theme	acids	1192:1196	arg1	production					1160:1169	the production	1156:1169	the production of short-chain fatty acids in normal mice	1156:1211	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	7	64	theme	acids	1192:1196	arg1	weight					1125:1130	the body weight	1116:1130	the body weight	1116:1130	The changes in the gut microbiota may affect the body weight, immune organ index and the production of short-chain fatty acids in normal mice.					
32926901	2	65	theme	pacificum	288:296	arg1	GPOP-1					322:327	GPOP-1	322:327	GPOP-1	322:327	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	65	theme	pacificum	288:296	arg1	polysaccharide					306:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide	249:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1)	249:328	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	66	theme	sinensis	363:370	arg1	CSP-1					388:392	CSP-1	388:392	CSP-1	388:392	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	66	theme	sinensis	363:370	arg1	polysaccharide					372:385	marine animal-derived Cereus sinensis polysaccharide	334:385	marine animal-derived Cereus sinensis polysaccharide (CSP-1)	334:393	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	3	67	theme	rRNA	513:516	arg1	sequencing					534:543	16S rRNA high-throughput sequencing	509:543	16S rRNA high-throughput sequencing	509:543	The composition and diversity of gut microbiota were evaluated using 16S rRNA high-throughput sequencing.					
32926901	5	68	theme	relative	798:805	arg1	abundance					807:815	the relative abundance	794:815	the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12	794:874	At the genus level, GPOP-1 increased the relative abundance of Bacteroides, Phascolarctobacterium, and decreased the relative abundance of Ruminococcus, Helicobacter, Allobaculum, Dorea and AF12.					
32926901	2	69	theme	Gelidium	279:286	arg1	GPOP-1					322:327	GPOP-1	322:327	GPOP-1	322:327	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	69	theme	Gelidium	279:286	arg1	polysaccharide					306:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide	249:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1)	249:328	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	1	70	theme	host	157:160	arg1	health					162:167	host health	157:167	host health	157:167	Many marine polysaccharides as prebiotics can promote host health by modulating gut microbiota.					
32926901	3	71	theme	microbiota	477:486	arg1	diversity					460:468	diversity	460:468	diversity	460:468	The composition and diversity of gut microbiota were evaluated using 16S rRNA high-throughput sequencing.					
32926901	3	71	theme	microbiota	477:486	arg1	composition					444:454	composition	444:454	composition	444:454	The composition and diversity of gut microbiota were evaluated using 16S rRNA high-throughput sequencing.					
32926901	4	72	theme	beneficial	660:669	arg1	bacteria					671:678	beneficial bacteria	660:678	beneficial bacteria	660:678	The results showed that GPOP-1 and CSP-1 altered the composition of the gut microbiota and promoted the growth of beneficial bacteria.					
32926901	8	73	theme	average	1269:1275	arg1	gain					1284:1287	average weight gain	1269:1287	average weight gain	1269:1287	Compared to the normal control group, GPOP-1 decreased average weight gain while CSP-1 increased average weight gain.					
32926901	8	74	theme	weight	1277:1282	arg1	gain					1284:1287	average weight gain	1269:1287	average weight gain	1269:1287	Compared to the normal control group, GPOP-1 decreased average weight gain while CSP-1 increased average weight gain.					
32926901	2	75	theme	plant-derived	265:277	arg1	GPOP-1					322:327	GPOP-1	322:327	GPOP-1	322:327	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	75	theme	plant-derived	265:277	arg1	polysaccharide					306:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide	249:319	purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1)	249:328	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
32926901	2	76	theme	normal	398:403	arg1	mice					405:408	normal mice	398:408	normal mice	398:408	This study investigated the beneficial effects of purified marine plant-derived Gelidium pacificum Okamura polysaccharide (GPOP-1) and marine animal-derived Cereus sinensis polysaccharide (CSP-1) on normal mice by modulating gut microbiota.					
34015511	0	0	theme	Composite	105:113	arg1	Properties					65:74	Physicochemical Properties	49:74	Physicochemical Properties of Hydroxyapatite/Cornstarch Composite	49:113	Low Frequency Dielectric and Optical Behavior on Physicochemical Properties of Hydroxyapatite/Cornstarch Composite.					
34015511	0	1	from	Dielectric	14:23	arg1	Properties					65:74	Physicochemical Properties	49:74	Physicochemical Properties of Hydroxyapatite/Cornstarch Composite	49:113	Low Frequency Dielectric and Optical Behavior on Physicochemical Properties of Hydroxyapatite/Cornstarch Composite.					
34015511	0	2	theme	Hydroxyapatite/Cornstarch	79:103	arg1	Composite					105:113	Hydroxyapatite/Cornstarch Composite	79:113	Hydroxyapatite/Cornstarch Composite	79:113	Low Frequency Dielectric and Optical Behavior on Physicochemical Properties of Hydroxyapatite/Cornstarch Composite.					
34015511	1	3	theme	%	324:324	arg1	proportion					278:287	the starch proportion	267:287	the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	267:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	1	4	theme	dielectric	188:197	arg1	properties					199:208	the physicochemical, optical and dielectric properties	155:208	the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	155:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	1	5	theme	30	292:293	arg1	proportion					278:287	the starch proportion	267:287	the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	267:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	5	6	theme	hydroxyapatite	705:718	arg1	center					731:736	hydroxyapatite nucleation center	705:736	hydroxyapatite nucleation center	705:736	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	6	7	theme	interfacial	984:994	arg1	interaction					996:1006	the stronger interfacial interaction	971:1006	the stronger interfacial interaction	971:1006	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	7	8	theme	design	1212:1217	arg1	potential					1183:1191	potential	1183:1191	potential of instrumentation design	1183:1217	The relationship among the physicochemical, optical and dielectric properties of the HA/Cs composite is studied in this work for potential of instrumentation design.					
34015511	6	9	theme	due	964:966	arg1	constant					924:931	dielectric constant	913:931	dielectric constant	913:931	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	9	theme	due	964:966	arg1	factor					939:944	loss factor	934:944	loss factor	934:944	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	9	theme	due	964:966	arg1	conductivity					950:961	conductivity	950:961	conductivity	950:961	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	9	theme	due	964:966	arg1	properties					901:910	higher dielectric properties	883:910	higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure	883:1051	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	5	10	theme	nucleation	720:729	arg1	center					731:736	hydroxyapatite nucleation center	705:736	hydroxyapatite nucleation center	705:736	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	11	theme	crystallized	681:692	arg1	HA/Cs					694:698	The highly crystallized HA/Cs	670:698	The highly crystallized HA/Cs with hydroxyapatite nucleation center	670:736	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	4	12	theme	composites	658:667	arg1	crystallinity					631:643	the higher crystallinity	620:643	the higher crystallinity of the HA/Cs composites	620:667	This increment trend results in the higher crystallinity of the HA/Cs composites.					
34015511	1	13	theme	40	296:297	arg1	proportion					278:287	the starch proportion	267:287	the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	267:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	6	14	with	composite	831:839	arg1	H2C8					872:875	H2C8	872:875	H2C8	872:875	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	14	with	composite	831:839	arg1	proportion					860:869	80 wt% starch proportion	846:869	80 wt% starch proportion (H2C8)	846:876	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	5	15	theme	band	803:806	arg1	properties					759:768	low optical properties	747:768	low optical properties (diffuse reflectance and optical band gap energy)	747:818	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	15	theme	band	803:806	arg1	energy					812:817	optical band gap energy	795:817	optical band gap energy	795:817	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	6	16	theme	crystalline	1031:1041	arg1	structure					1043:1051	close-packed HA/Cs crystalline structure	1012:1051	close-packed HA/Cs crystalline structure	1012:1051	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	7	17	theme	HA/Cs	1139:1143	arg1	composite					1145:1153	the HA/Cs composite	1135:1153	the HA/Cs composite	1135:1153	The relationship among the physicochemical, optical and dielectric properties of the HA/Cs composite is studied in this work for potential of instrumentation design.					
34015511	0	18	from	Behavior	37:44	arg1	Properties					65:74	Physicochemical Properties	49:74	Physicochemical Properties of Hydroxyapatite/Cornstarch Composite	49:113	Low Frequency Dielectric and Optical Behavior on Physicochemical Properties of Hydroxyapatite/Cornstarch Composite.					
34015511	7	19	theme	composite	1145:1153	arg1	properties					1121:1130	the physicochemical, optical and dielectric properties	1077:1130	the physicochemical, optical and dielectric properties of the HA/Cs composite	1077:1153	The relationship among the physicochemical, optical and dielectric properties of the HA/Cs composite is studied in this work for potential of instrumentation design.					
34015511	5	20	theme	gap	808:810	arg1	properties					759:768	low optical properties	747:768	low optical properties (diffuse reflectance and optical band gap energy)	747:818	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	20	theme	gap	808:810	arg1	energy					812:817	optical band gap energy	795:817	optical band gap energy	795:817	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	2	21	theme	HA/Cs	357:361	arg1	composites					363:372	The HA/Cs composites	353:372	The HA/Cs composites	353:372	The HA/Cs composites have been characterized via FTIR, XRD, DRS and impedance analyzer.					
34015511	0	22	theme	Frequency	4:12	arg1	Dielectric					14:23	Low Frequency Dielectric	0:23	Low Frequency Dielectric	0:23	Low Frequency Dielectric and Optical Behavior on Physicochemical Properties of Hydroxyapatite/Cornstarch Composite.					
34015511	6	23	theme	loss	934:937	arg1	factor					939:944	loss factor	934:944	loss factor	934:944	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	23	theme	loss	934:937	arg1	properties					901:910	higher dielectric properties	883:910	higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure	883:1051	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	1	24	theme	90 wt	319:323	arg1	%					324:324	90 wt%	319:324	90 wt%	319:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	0	25	theme	Low	0:2	arg1	Dielectric					14:23	Low Frequency Dielectric	0:23	Low Frequency Dielectric	0:23	Low Frequency Dielectric and Optical Behavior on Physicochemical Properties of Hydroxyapatite/Cornstarch Composite.					
34015511	7	26	theme	physicochemical	1081:1095	arg1	properties					1121:1130	the physicochemical, optical and dielectric properties	1077:1130	the physicochemical, optical and dielectric properties of the HA/Cs composite	1077:1153	The relationship among the physicochemical, optical and dielectric properties of the HA/Cs composite is studied in this work for potential of instrumentation design.					
34015511	6	27	theme	composite	831:839	arg1	HA/Cs					825:829	The HA/Cs	821:829	The HA/Cs composite with 80 wt% starch proportion (H2C8)	821:876	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	1	28	theme	hydroxyapatite/cornstarch	217:241	arg1	composites					251:260	the hydroxyapatite/cornstarch (HA/Cs) composites	213:260	the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	213:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	1	29	theme	50	300:301	arg1	proportion					278:287	the starch proportion	267:287	the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	267:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	5	30	dep	properties	759:768	arg1	properties					759:768	low optical properties	747:768	low optical properties (diffuse reflectance and optical band gap energy)	747:818	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	30	dep	properties	759:768	arg1	energy					812:817	optical band gap energy	795:817	optical band gap energy	795:817	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	30	dep	properties	759:768	arg1	reflectance					779:789	diffuse reflectance	771:789	diffuse reflectance	771:789	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	3	31	theme	hydroxyapatite	512:525	arg1	nanoparticles					527:539	the hydroxyapatite nanoparticles	508:539	the hydroxyapatite nanoparticles	508:539	This work depicts that the strong interaction is exhibited between the hydroxyapatite nanoparticles and starch as the starch proportion increases.					
34015511	5	32	with	HA/Cs	694:698	arg1	center					731:736	hydroxyapatite nucleation center	705:736	hydroxyapatite nucleation center	705:736	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	1	33	with	composites	251:260	arg1	proportion					278:287	the starch proportion	267:287	the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	267:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	6	34	theme	starch	853:858	arg1	H2C8					872:875	H2C8	872:875	H2C8	872:875	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	34	theme	starch	853:858	arg1	proportion					860:869	80 wt% starch proportion	846:869	80 wt% starch proportion (H2C8)	846:876	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	35	theme	dielectric	890:899	arg1	constant					924:931	dielectric constant	913:931	dielectric constant	913:931	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	35	theme	dielectric	890:899	arg1	factor					939:944	loss factor	934:944	loss factor	934:944	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	35	theme	dielectric	890:899	arg1	conductivity					950:961	conductivity	950:961	conductivity	950:961	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	35	theme	dielectric	890:899	arg1	properties					901:910	higher dielectric properties	883:910	higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure	883:1051	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	1	36	theme	HA/Cs	244:248	arg1	composites					251:260	the hydroxyapatite/cornstarch (HA/Cs) composites	213:260	the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	213:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	5	37	theme	diffuse	771:777	arg1	properties					759:768	low optical properties	747:768	low optical properties (diffuse reflectance and optical band gap energy)	747:818	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	37	theme	diffuse	771:777	arg1	reflectance					779:789	diffuse reflectance	771:789	diffuse reflectance	771:789	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	1	38	theme	60	304:305	arg1	proportion					278:287	the starch proportion	267:287	the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	267:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	6	39	theme	%	851:851	arg1	H2C8					872:875	H2C8	872:875	H2C8	872:875	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	39	theme	%	851:851	arg1	proportion					860:869	80 wt% starch proportion	846:869	80 wt% starch proportion (H2C8)	846:876	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	3	40	theme	starch	559:564	arg1	increases					577:585	the starch proportion increases	555:585	the starch proportion increases	555:585	This work depicts that the strong interaction is exhibited between the hydroxyapatite nanoparticles and starch as the starch proportion increases.					
34015511	6	41	theme	HA/Cs	1025:1029	arg1	structure					1043:1051	close-packed HA/Cs crystalline structure	1012:1051	close-packed HA/Cs crystalline structure	1012:1051	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	0	42	theme	Optical	29:35	arg1	Behavior					37:44	Optical Behavior	29:44	Optical Behavior	29:44	Low Frequency Dielectric and Optical Behavior on Physicochemical Properties of Hydroxyapatite/Cornstarch Composite.					
34015511	6	43	dep	properties	901:910	arg1	constant					924:931	dielectric constant	913:931	dielectric constant	913:931	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	43	dep	properties	901:910	arg1	factor					939:944	loss factor	934:944	loss factor	934:944	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	43	dep	properties	901:910	arg1	properties					901:910	higher dielectric properties	883:910	higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure	883:1051	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	43	dep	properties	901:910	arg1	conductivity					950:961	conductivity	950:961	conductivity	950:961	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	2	44	theme	impedance	421:429	arg1	analyzer					431:438	impedance analyzer	421:438	impedance analyzer	421:438	The HA/Cs composites have been characterized via FTIR, XRD, DRS and impedance analyzer.					
34015511	6	45	theme	80 wt	846:850	arg1	H2C8					872:875	H2C8	872:875	H2C8	872:875	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	45	theme	80 wt	846:850	arg1	proportion					860:869	80 wt% starch proportion	846:869	80 wt% starch proportion (H2C8)	846:876	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	3	46	theme	proportion	566:575	arg1	increases					577:585	the starch proportion increases	555:585	the starch proportion increases	555:585	This work depicts that the strong interaction is exhibited between the hydroxyapatite nanoparticles and starch as the starch proportion increases.					
34015511	1	47	theme	composites	251:260	arg1	properties					199:208	the physicochemical, optical and dielectric properties	155:208	the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	155:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	0	48	theme	Physicochemical	49:63	arg1	Properties					65:74	Physicochemical Properties	49:74	Physicochemical Properties of Hydroxyapatite/Cornstarch Composite	49:113	Low Frequency Dielectric and Optical Behavior on Physicochemical Properties of Hydroxyapatite/Cornstarch Composite.					
34015511	1	49	theme	70	308:309	arg1	proportion					278:287	the starch proportion	267:287	the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	267:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	5	50	theme	optical	795:801	arg1	properties					759:768	low optical properties	747:768	low optical properties (diffuse reflectance and optical band gap energy)	747:818	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	50	theme	optical	795:801	arg1	energy					812:817	optical band gap energy	795:817	optical band gap energy	795:817	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	6	51	theme	dielectric	913:922	arg1	constant					924:931	dielectric constant	913:931	dielectric constant	913:931	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	51	theme	dielectric	913:922	arg1	properties					901:910	higher dielectric properties	883:910	higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure	883:1051	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	52	theme	higher	883:888	arg1	constant					924:931	dielectric constant	913:931	dielectric constant	913:931	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	52	theme	higher	883:888	arg1	factor					939:944	loss factor	934:944	loss factor	934:944	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	52	theme	higher	883:888	arg1	conductivity					950:961	conductivity	950:961	conductivity	950:961	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	6	52	theme	higher	883:888	arg1	properties					901:910	higher dielectric properties	883:910	higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure	883:1051	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	7	53	theme	optical	1098:1104	arg1	properties					1121:1130	the physicochemical, optical and dielectric properties	1077:1130	the physicochemical, optical and dielectric properties of the HA/Cs composite	1077:1153	The relationship among the physicochemical, optical and dielectric properties of the HA/Cs composite is studied in this work for potential of instrumentation design.					
34015511	7	54	theme	instrumentation	1196:1210	arg1	design					1212:1217	instrumentation design	1196:1217	instrumentation design	1196:1217	The relationship among the physicochemical, optical and dielectric properties of the HA/Cs composite is studied in this work for potential of instrumentation design.					
34015511	1	55	from	investigation	119:131	arg1	relationship					136:147	relationship	136:147	relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	136:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	1	56	theme	80	312:313	arg1	proportion					278:287	the starch proportion	267:287	the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	267:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	4	57	theme	HA/Cs	652:656	arg1	composites					658:667	the HA/Cs composites	648:667	the HA/Cs composites	648:667	This increment trend results in the higher crystallinity of the HA/Cs composites.					
34015511	1	58	theme	physicochemical	159:173	arg1	properties					199:208	the physicochemical, optical and dielectric properties	155:208	the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	155:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	5	59	theme	low	747:749	arg1	energy					812:817	optical band gap energy	795:817	optical band gap energy	795:817	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	59	theme	low	747:749	arg1	properties					759:768	low optical properties	747:768	low optical properties (diffuse reflectance and optical band gap energy)	747:818	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	59	theme	low	747:749	arg1	reflectance					779:789	diffuse reflectance	771:789	diffuse reflectance	771:789	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	6	60	theme	close-packed	1012:1023	arg1	structure					1043:1051	close-packed HA/Cs crystalline structure	1012:1051	close-packed HA/Cs crystalline structure	1012:1051	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
34015511	4	61	theme	increment	593:601	arg1	trend					603:607	This increment trend	588:607	This increment trend	588:607	This increment trend results in the higher crystallinity of the HA/Cs composites.					
34015511	3	62	theme	strong	468:473	arg1	interaction					475:485	the strong interaction	464:485	the strong interaction	464:485	This work depicts that the strong interaction is exhibited between the hydroxyapatite nanoparticles and starch as the starch proportion increases.					
34015511	4	63	theme	higher	624:629	arg1	crystallinity					631:643	the higher crystallinity	620:643	the higher crystallinity of the HA/Cs composites	620:667	This increment trend results in the higher crystallinity of the HA/Cs composites.					
34015511	1	64	theme	starch	271:276	arg1	proportion					278:287	the starch proportion	267:287	the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	267:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	5	65	theme	optical	751:757	arg1	energy					812:817	optical band gap energy	795:817	optical band gap energy	795:817	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	65	theme	optical	751:757	arg1	properties					759:768	low optical properties	747:768	low optical properties (diffuse reflectance and optical band gap energy)	747:818	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	5	65	theme	optical	751:757	arg1	reflectance					779:789	diffuse reflectance	771:789	diffuse reflectance	771:789	The highly crystallized HA/Cs with hydroxyapatite nucleation center presents low optical properties (diffuse reflectance and optical band gap energy).					
34015511	7	66	theme	dielectric	1110:1119	arg1	properties					1121:1130	the physicochemical, optical and dielectric properties	1077:1130	the physicochemical, optical and dielectric properties of the HA/Cs composite	1077:1153	The relationship among the physicochemical, optical and dielectric properties of the HA/Cs composite is studied in this work for potential of instrumentation design.					
34015511	1	67	theme	optical	176:182	arg1	properties					199:208	the physicochemical, optical and dielectric properties	155:208	the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt%	155:324	An investigation on relationship among the physicochemical, optical and dielectric properties of the hydroxyapatite/cornstarch (HA/Cs) composites with the starch proportion of 30, 40, 50, 60, 70, 80 and 90 wt% is presented in this work.					
34015511	6	68	theme	stronger	975:982	arg1	interaction					996:1006	the stronger interfacial interaction	971:1006	the stronger interfacial interaction	971:1006	The HA/Cs composite with 80 wt% starch proportion (H2C8) show higher dielectric properties (dielectric constant, loss factor and conductivity) due to the stronger interfacial interaction and close-packed HA/Cs crystalline structure.					
32424941	9	0	theme	acids	1546:1550	arg1	higher					1633:1638	higher	1633:1638	higher	1633:1638	The contents of short-chain fatty acids after TFPS supplementation, both in cecal contents and feces, were significantly higher than those of the NC group.					
32424941	9	0	theme	acids	1546:1550	arg1	contents					1516:1523	The contents	1512:1523	The contents of short-chain fatty acids	1512:1550	The contents of short-chain fatty acids after TFPS supplementation, both in cecal contents and feces, were significantly higher than those of the NC group.					
32424941	9	1	theme	TFPS	1558:1561	arg1	supplementation					1563:1577	TFPS supplementation	1558:1577	TFPS supplementation	1558:1577	The contents of short-chain fatty acids after TFPS supplementation, both in cecal contents and feces, were significantly higher than those of the NC group.					
32424941	4	2	theme	6-week-old	565:574	arg1	mice					588:591	twenty 6-week-old BALB/c male mice	558:591	twenty 6-week-old BALB/c male mice	558:591	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	6	3	theme	mRNA	1158:1161	arg1	expression					1129:1138	the expression	1125:1138	the expression of TLR4 and TNF-α mRNA	1125:1161	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	13	4	theme	functional	2063:2072	arg1	TFPS					2035:2038	TFPS	2035:2038	TFPS	2035:2038	Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.					
32424941	13	4	theme	functional	2063:2072	arg1	food					2074:2077	a functional food	2061:2077	a functional food	2061:2077	Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.					
32424941	6	5	theme	significant	1064:1074	arg1	difference					1076:1085	a significant difference	1062:1085	a significant difference between TFPS supplement	1062:1109	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	5	6	theme	jejunum	767:773	arg1	observation					752:762	Histomorphology observation	736:762	Histomorphology observation of jejunum and colons	736:784	Histomorphology observation of jejunum and colons showed that TFPS maintained the adequate gut barrier.					
32424941	7	7	theme	mice	1296:1299	arg1	feces					1287:1291	feces	1287:1291	feces of mice	1287:1299	16S rDNA amplicons sequencing was applied to measure the compositions of gut microbiota from feces of mice.					
32424941	4	8	theme	normal	632:637	arg1	group					647:651	normal control group	632:651	normal control group	632:651	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	4	8	theme	normal	632:637	arg1	group					657:661	NC group	654:661	NC group	654:661	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	2	9	theme	important	299:307	arg1	gut					283:285	the gut	279:285	the gut	279:285	However, dietary polysaccharides cannot be digested by the human alimentary tract, thus the gut is the most important location where polysaccharides play their role.					
32424941	2	9	theme	important	299:307	arg1	location					309:316	the most important location	290:316	the most important location where polysaccharides play their role	290:354	However, dietary polysaccharides cannot be digested by the human alimentary tract, thus the gut is the most important location where polysaccharides play their role.					
32424941	5	10	theme	colons	779:784	arg1	observation					752:762	Histomorphology observation	736:762	Histomorphology observation of jejunum and colons	736:784	Histomorphology observation of jejunum and colons showed that TFPS maintained the adequate gut barrier.					
32424941	12	11	theme	intestinal	1904:1913	arg1	health					1915:1920	human intestinal health	1898:1920	human intestinal health	1898:1920	PRACTICAL APPLICATION: Dietary polysaccharides improve human intestinal health.					
32424941	6	12	theme	mRNA	1046:1049	arg1	level					1051:1055	mRNA level	1046:1055	mRNA level	1046:1055	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	4	13	dep	10	726:727	arg1	each					729:732	each	729:732	each	729:732	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	8	14	theme	relative	1335:1342	arg1	abundances					1344:1353	similar relative abundances	1327:1353	similar relative abundances	1327:1353	TFPS treatment exhibited similar relative abundances in Bacteroidetes and Firmicutes; however, it decreased the relative abundance of Akkermansia and increased that of Lactobacillus compared with the NC group.					
32424941	7	15	theme	microbiota	1271:1280	arg1	compositions					1251:1262	the compositions	1247:1262	the compositions of gut microbiota from feces of mice	1247:1299	16S rDNA amplicons sequencing was applied to measure the compositions of gut microbiota from feces of mice.					
32424941	0	16	theme	BALB/c	121:126	arg1	mice					128:131	BALB/c mice	121:131	BALB/c mice	121:131	Effect of long-term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice.					
32424941	12	17	theme	Dietary	1866:1872	arg1	polysaccharides					1874:1888	Dietary polysaccharides	1866:1888	Dietary polysaccharides	1866:1888	PRACTICAL APPLICATION: Dietary polysaccharides improve human intestinal health.					
32424941	5	18	theme	adequate	818:825	arg1	barrier					831:837	the adequate gut barrier	814:837	the adequate gut barrier	814:837	Histomorphology observation of jejunum and colons showed that TFPS maintained the adequate gut barrier.					
32424941	7	19	from	feces	1287:1291	arg1	compositions					1251:1262	the compositions	1247:1262	the compositions of gut microbiota from feces of mice	1247:1299	16S rDNA amplicons sequencing was applied to measure the compositions of gut microbiota from feces of mice.					
32424941	2	20	theme	human	250:254	arg1	tract					267:271	the human alimentary tract	246:271	the human alimentary tract	246:271	However, dietary polysaccharides cannot be digested by the human alimentary tract, thus the gut is the most important location where polysaccharides play their role.					
32424941	4	21	theme	NC	654:655	arg1	group					647:651	normal control group	632:651	normal control group	632:651	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	4	21	theme	NC	654:655	arg1	group					657:661	NC group	654:661	NC group	654:661	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	1	22	theme	various	155:161	arg1	functions					180:188	various health-promoting functions	155:188	various health-promoting functions	155:188	Polysaccharides have various health-promoting functions.					
32424941	6	23	theme	junction	900:907	arg1	proteins					909:916	colonic tight junction proteins	886:916	colonic tight junction proteins	886:916	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	12	24	dep	APPLICATION	1853:1863	arg1	improve					1890:1896	improve	1890:1896	improve human intestinal health	1890:1920	PRACTICAL APPLICATION: Dietary polysaccharides improve human intestinal health.					
32424941	0	25	theme	polysaccharides	69:83	arg1	consumption					20:30	long-term consumption	10:30	long-term consumption of tea (Camellia sinensis L.) flower polysaccharides	10:83	Effect of long-term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice.					
32424941	13	26	theme	safe	1957:1960	arg1	TFPS					1951:1954	TFPS	1951:1954	TFPS	1951:1954	Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.					
32424941	13	26	theme	safe	1957:1960	arg1	components					1979:1988	safe and healthy food components	1957:1988	safe and healthy food components	1957:1988	Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.					
32424941	6	27	theme	colonic	886:892	arg1	proteins					909:916	colonic tight junction proteins	886:916	colonic tight junction proteins	886:916	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	8	28	theme	relative	1414:1421	arg1	abundance					1423:1431	the relative abundance	1410:1431	the relative abundance of Akkermansia	1410:1446	TFPS treatment exhibited similar relative abundances in Bacteroidetes and Firmicutes; however, it decreased the relative abundance of Akkermansia and increased that of Lactobacillus compared with the NC group.					
32424941	4	29	theme	TFPS	703:706	arg1	/day					695:698	/day	695:698	/day of TFPS for 13 weeks (n = 10 each)	695:733	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	4	29	theme	TFPS	703:706	arg1	200 mg/kg					672:680	200 mg/kg	672:680	200 mg/kg (body weight)	672:694	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	4	29	theme	TFPS	703:706	arg1	weight					688:693	body weight	683:693	body weight	683:693	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	13	30	theme	healthy	1966:1972	arg1	food					1974:1977	healthy food	1966:1977	healthy food	1966:1977	Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.					
32424941	9	31	dep	supplementation	1563:1577	arg1	both					1580:1583	both	1580:1583	both	1580:1583	The contents of short-chain fatty acids after TFPS supplementation, both in cecal contents and feces, were significantly higher than those of the NC group.					
32424941	6	32	dep	claudin5	984:991	arg1	2.91 ± 0.44 compared					994:1013	2.91 ± 0.44 compared	994:1013	2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01	994:1040	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	3	33	theme	intestinal	436:445	arg1	health					447:452	intestinal health	436:452	intestinal health	436:452	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study.					
32424941	0	34	theme	long-term	10:18	arg1	consumption					20:30	long-term consumption	10:30	long-term consumption of tea (Camellia sinensis L.) flower polysaccharides	10:83	Effect of long-term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice.					
32424941	7	35	theme	rDNA	1198:1201	arg1	sequencing					1213:1222	16S rDNA amplicons sequencing	1194:1222	16S rDNA amplicons sequencing	1194:1222	16S rDNA amplicons sequencing was applied to measure the compositions of gut microbiota from feces of mice.					
32424941	4	36	theme	=	724:724	arg1	n					722:722	n	722:722	n = 10 each	722:732	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	11	37	theme	adaptive	1816:1823	arg1	tolerance					1832:1840	intestinal adaptive immune tolerance	1805:1840	intestinal adaptive immune tolerance	1805:1840	These results suggest that TFPS is beneficial to intestinal health and can improve intestinal adaptive immune tolerance.					
32424941	4	38	with	TFPS	493:496	arg1	weight					517:522	the molecular weight	503:522	the molecular weight of 1,316.29 kDa	503:538	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	4	39	theme	molecular	507:515	arg1	weight					517:522	the molecular weight	503:522	the molecular weight of 1,316.29 kDa	503:538	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	8	40	theme	NC	1502:1503	arg1	group					1505:1509	the NC group	1498:1509	the NC group	1498:1509	TFPS treatment exhibited similar relative abundances in Bacteroidetes and Firmicutes; however, it decreased the relative abundance of Akkermansia and increased that of Lactobacillus compared with the NC group.					
32424941	8	41	theme	similar	1327:1333	arg1	abundances					1344:1353	similar relative abundances	1327:1353	similar relative abundances	1327:1353	TFPS treatment exhibited similar relative abundances in Bacteroidetes and Firmicutes; however, it decreased the relative abundance of Akkermansia and increased that of Lactobacillus compared with the NC group.					
32424941	11	42	theme	intestinal	1771:1780	arg1	health					1782:1787	intestinal health	1771:1787	intestinal health	1771:1787	These results suggest that TFPS is beneficial to intestinal health and can improve intestinal adaptive immune tolerance.					
32424941	4	43	dep	weeks	715:719	arg1	n					722:722	n	722:722	n = 10 each	722:732	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	9	44	theme	fatty	1540:1544	arg1	acids					1546:1550	short-chain fatty acids	1528:1550	short-chain fatty acids	1528:1550	The contents of short-chain fatty acids after TFPS supplementation, both in cecal contents and feces, were significantly higher than those of the NC group.					
32424941	3	45	from	TFPS	427:430	arg1	polysaccharides					371:385	polysaccharides	371:385	polysaccharides from tea (Camellia sinensis L.) flower (TFPS)	371:431	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study.					
32424941	3	45	from	TFPS	427:430	arg1	effect					361:366	The effect	357:366	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health	357:452	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study.					
32424941	3	46	theme	present	478:484	arg1	study					486:490	the present study	474:490	the present study	474:490	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study.					
32424941	1	47	contain	have	150:153	arg1	Polysaccharides					134:148	Polysaccharides	134:148	Polysaccharides	134:148	Polysaccharides have various health-promoting functions.					
32424941	1	47	contain	have	150:153	arg2	functions					180:188	various health-promoting functions	155:188	various health-promoting functions	155:188	Polysaccharides have various health-promoting functions.					
32424941	6	48	theme	TNF-α	1152:1156	arg1	mRNA					1158:1161	TNF-α mRNA	1152:1161	TNF-α mRNA	1152:1161	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	1	49	theme	health-promoting	163:178	arg1	functions					180:188	various health-promoting functions	155:188	various health-promoting functions	155:188	Polysaccharides have various health-promoting functions.					
32424941	4	50	theme	chow	621:624	arg1	diet					626:629	a chow diet	619:629	a chow diet (normal control group, NC group)	619:662	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	6	51	theme	TLR4	1143:1146	arg1	expression					1129:1138	the expression	1125:1138	the expression of TLR4 and TNF-α mRNA	1125:1161	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	9	52	theme	cecal	1588:1592	arg1	contents					1594:1601	cecal contents	1588:1601	cecal contents	1588:1601	The contents of short-chain fatty acids after TFPS supplementation, both in cecal contents and feces, were significantly higher than those of the NC group.					
32424941	12	53	theme	human	1898:1902	arg1	health					1915:1920	human intestinal health	1898:1920	human intestinal health	1898:1920	PRACTICAL APPLICATION: Dietary polysaccharides improve human intestinal health.					
32424941	10	54	theme	IgA	1706:1708	arg1	production					1710:1719	IgA production	1706:1719	IgA production	1706:1719	Besides, TFPS significantly increased IgA production.					
32424941	5	55	theme	Histomorphology	736:750	arg1	observation					752:762	Histomorphology observation	736:762	Histomorphology observation of jejunum and colons	736:784	Histomorphology observation of jejunum and colons showed that TFPS maintained the adequate gut barrier.					
32424941	12	56	theme	PRACTICAL	1843:1851	arg1	APPLICATION					1853:1863	PRACTICAL APPLICATION	1843:1863	PRACTICAL APPLICATION: Dietary polysaccharides improve human intestinal health.	1843:1921	PRACTICAL APPLICATION: Dietary polysaccharides improve human intestinal health.					
32424941	3	57	from	effect	361:366	arg1	TFPS					427:430	tea (Camellia sinensis L.) flower (TFPS)	392:431	tea (Camellia sinensis L.) flower (TFPS)	392:431	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study.					
32424941	3	57	from	effect	361:366	arg1	health					447:452	intestinal health	436:452	intestinal health	436:452	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study.					
32424941	6	58	theme	qPCR	840:843	arg1	analysis					845:852	qPCR analysis	840:852	qPCR analysis	840:852	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	7	59	theme	gut	1267:1269	arg1	microbiota					1271:1280	gut microbiota	1267:1280	gut microbiota	1267:1280	16S rDNA amplicons sequencing was applied to measure the compositions of gut microbiota from feces of mice.					
32424941	5	60	theme	gut	827:829	arg1	barrier					831:837	the adequate gut barrier	814:837	the adequate gut barrier	814:837	Histomorphology observation of jejunum and colons showed that TFPS maintained the adequate gut barrier.					
32424941	2	61	theme	alimentary	256:265	arg1	tract					267:271	the human alimentary tract	246:271	the human alimentary tract	246:271	However, dietary polysaccharides cannot be digested by the human alimentary tract, thus the gut is the most important location where polysaccharides play their role.					
32424941	6	62	theme	claudin1	921:928	arg1	expression					872:881	the expression	868:881	the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01)	868:1041	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	6	63	theme	TFPS	1095:1098	arg1	supplement					1100:1109	TFPS supplement	1095:1109	TFPS supplement	1095:1109	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	4	64	theme	control	639:645	arg1	group					647:651	normal control group	632:651	normal control group	632:651	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	4	64	theme	control	639:645	arg1	group					657:661	NC group	654:661	NC group	654:661	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	0	65	theme	flower	62:67	arg1	polysaccharides					69:83	tea (Camellia sinensis L.) flower polysaccharides	35:83	tea (Camellia sinensis L.) flower polysaccharides	35:83	Effect of long-term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice.					
32424941	6	66	theme	proteins	909:916	arg1	expression					872:881	the expression	868:881	the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01)	868:1041	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	9	67	theme	NC	1658:1659	arg1	group					1661:1665	the NC group	1654:1665	the NC group	1654:1665	The contents of short-chain fatty acids after TFPS supplementation, both in cecal contents and feces, were significantly higher than those of the NC group.					
32424941	13	68	theme	gut	1994:1996	arg1	health					1998:2003	gut health	1994:2003	gut health	1994:2003	Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.					
32424941	6	69	theme	tight	894:898	arg1	proteins					909:916	colonic tight junction proteins	886:916	colonic tight junction proteins	886:916	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	6	70	dep	claudin1	921:928	arg1	1.29 ± 0.15 compared					931:950	1.29 ± 0.15 compared	931:950	1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05	931:977	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	0	71	theme	intestinal	100:109	arg1	health					111:116	intestinal health	100:116	intestinal health	100:116	Effect of long-term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice.					
32424941	6	72	theme	claudin5	984:991	arg1	expression					872:881	the expression	868:881	the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01)	868:1041	qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF-α mRNA was not changed statistically.					
32424941	8	73	theme	Akkermansia	1436:1446	arg1	abundance					1423:1431	the relative abundance	1410:1431	the relative abundance of Akkermansia	1410:1446	TFPS treatment exhibited similar relative abundances in Bacteroidetes and Firmicutes; however, it decreased the relative abundance of Akkermansia and increased that of Lactobacillus compared with the NC group.					
32424941	3	74	dep	Camellia	397:404	arg1	L.					415:416	Camellia sinensis L.	397:416	Camellia sinensis L.	397:416	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study.					
32424941	4	75	theme	BALB/c	576:581	arg1	mice					588:591	twenty 6-week-old BALB/c male mice	558:591	twenty 6-week-old BALB/c male mice	558:591	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	13	76	from	effect	1941:1946	arg1	health					1998:2003	gut health	1994:2003	gut health	1994:2003	Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.					
32424941	11	77	theme	intestinal	1805:1814	arg1	tolerance					1832:1840	intestinal adaptive immune tolerance	1805:1840	intestinal adaptive immune tolerance	1805:1840	These results suggest that TFPS is beneficial to intestinal health and can improve intestinal adaptive immune tolerance.					
32424941	13	78	theme	food	1974:1977	arg1	TFPS					1951:1954	TFPS	1951:1954	TFPS	1951:1954	Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.					
32424941	13	78	theme	food	1974:1977	arg1	components					1979:1988	safe and healthy food components	1957:1988	safe and healthy food components	1957:1988	Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.					
32424941	7	79	theme	amplicons	1203:1211	arg1	sequencing					1213:1222	16S rDNA amplicons sequencing	1194:1222	16S rDNA amplicons sequencing	1194:1222	16S rDNA amplicons sequencing was applied to measure the compositions of gut microbiota from feces of mice.					
32424941	3	80	theme	tea	392:394	arg1	TFPS					427:430	tea (Camellia sinensis L.) flower (TFPS)	392:431	tea (Camellia sinensis L.) flower (TFPS)	392:431	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study.					
32424941	4	81	theme	1,316.29 kDa	527:538	arg1	weight					517:522	the molecular weight	503:522	the molecular weight of 1,316.29 kDa	503:538	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	3	82	theme	flower	419:424	arg1	TFPS					427:430	tea (Camellia sinensis L.) flower (TFPS)	392:431	tea (Camellia sinensis L.) flower (TFPS)	392:431	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study.					
32424941	0	83	theme	consumption	20:30	arg1	Effect					0:5	Effect	0:5	Effect of long-term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice.	0:132	Effect of long-term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice.					
32424941	9	84	theme	short-chain	1528:1538	arg1	acids					1546:1550	short-chain fatty acids	1528:1550	short-chain fatty acids	1528:1550	The contents of short-chain fatty acids after TFPS supplementation, both in cecal contents and feces, were significantly higher than those of the NC group.					
32424941	7	85	theme	16S	1194:1196	arg1	sequencing					1213:1222	16S rDNA amplicons sequencing	1194:1222	16S rDNA amplicons sequencing	1194:1222	16S rDNA amplicons sequencing was applied to measure the compositions of gut microbiota from feces of mice.					
32424941	3	86	theme	polysaccharides	371:385	arg1	effect					361:366	The effect	357:366	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health	357:452	The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study.					
32424941	4	87	theme	body	683:686	arg1	200 mg/kg					672:680	200 mg/kg	672:680	200 mg/kg (body weight)	672:694	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	4	87	theme	body	683:686	arg1	weight					688:693	body weight	683:693	body weight	683:693	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	0	88	theme	tea	35:37	arg1	polysaccharides					69:83	tea (Camellia sinensis L.) flower polysaccharides	35:83	tea (Camellia sinensis L.) flower polysaccharides	35:83	Effect of long-term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice.					
32424941	4	89	dep	=	724:724	arg1	10					726:727	10	726:727	10	726:727	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	0	90	dep	Camellia	40:47	arg1	L.					58:59	Camellia sinensis L.	40:59	Camellia sinensis L.	40:59	Effect of long-term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice.					
32424941	11	91	theme	immune	1825:1830	arg1	tolerance					1832:1840	intestinal adaptive immune tolerance	1805:1840	intestinal adaptive immune tolerance	1805:1840	These results suggest that TFPS is beneficial to intestinal health and can improve intestinal adaptive immune tolerance.					
32424941	13	92	theme	TFPS	1951:1954	arg1	effect					1941:1946	the effect	1937:1946	the effect of TFPS, safe and healthy food components, on gut health	1937:2003	Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.					
32424941	4	93	theme	male	583:586	arg1	mice					588:591	twenty 6-week-old BALB/c male mice	558:591	twenty 6-week-old BALB/c male mice	558:591	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	2	94	theme	dietary	200:206	arg1	polysaccharides					208:222	dietary polysaccharides	200:222	dietary polysaccharides	200:222	However, dietary polysaccharides cannot be digested by the human alimentary tract, thus the gut is the most important location where polysaccharides play their role.					
32424941	8	95	theme	TFPS	1302:1305	arg1	treatment					1307:1315	TFPS treatment	1302:1315	TFPS treatment	1302:1315	TFPS treatment exhibited similar relative abundances in Bacteroidetes and Firmicutes; however, it decreased the relative abundance of Akkermansia and increased that of Lactobacillus compared with the NC group.					
32424941	4	96	dep	diet	626:629	arg1	group					647:651	normal control group	632:651	normal control group	632:651	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32424941	4	96	dep	diet	626:629	arg1	group					657:661	NC group	654:661	NC group	654:661	TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6-week-old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each).					
32871118	0	0	theme	sanghuang	109:117	arg1	mycelia					119:125	Sanghuangporus sanghuang mycelia	94:125	Sanghuangporus sanghuang mycelia	94:125	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	3	1	with	residues	548:555	arg1	points					575:580	two branched points	562:580	two branched points at O-6	562:587	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	1	2	theme	Sephacryl	270:278	arg1	columns					286:292	Sephacryl S-100 columns	270:292	Sephacryl S-100 columns	270:292	A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns.					
32871118	0	3	theme	Sanghuangporus	94:107	arg1	mycelia					119:125	Sanghuangporus sanghuang mycelia	94:125	Sanghuangporus sanghuang mycelia	94:125	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	0	4	from	characterization	11:26	arg1	mycelia					119:125	Sanghuangporus sanghuang mycelia	94:125	Sanghuangporus sanghuang mycelia	94:125	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	6	5	theme	in	1144:1145	arg1	resistance					1161:1170	in vitro insulin resistance	1144:1170	in vitro insulin resistance of HepG2 cells	1144:1185	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
32871118	3	6	from	O-6	585:587	arg1	points					575:580	two branched points	562:580	two branched points at O-6	562:587	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	1	7	theme	S-100	280:284	arg1	columns					286:292	Sephacryl S-100 columns	270:292	Sephacryl S-100 columns	270:292	A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns.					
32871118	5	8	theme	force	996:1000	arg1	microscopy					1002:1011	atomic force microscopy	989:1011	atomic force microscopy	989:1011	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	5	9	theme	atomic	989:994	arg1	microscopy					1002:1011	atomic force microscopy	989:1011	atomic force microscopy	989:1011	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	3	10	theme	methylation	399:409	arg1	results					388:394	The results	384:394	The results of methylation and 2D-NMR analysis	384:429	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	4	11	theme	1,4-linked	670:679	arg1	α-D-Glcp					681:688	1,4-linked α-D-Glcp	670:688	1,4-linked α-D-Glcp	670:688	The two branches were composed of 1,4-linked α-D-Glcp terminated with α-D-Glcp, 1,4-linked α-D-Glcp and 1,4-linked β-Galp terminated by α-D-Glcp.					
32871118	3	12	link	1,4-linked	512:521	arg1	residues					548:555	1,4-linked α-d-glucopyranose (Glcp) residues	512:555	1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6	512:587	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	4	13	theme	1,4-linked	624:633	arg1	α-D-Glcp					635:642	1,4-linked α-D-Glcp	624:642	1,4-linked α-D-Glcp terminated with α-D-Glcp, 1,4-linked α-D-Glcp and 1,4-linked β-Galp terminated by α-D-Glcp	624:733	The two branches were composed of 1,4-linked α-D-Glcp terminated with α-D-Glcp, 1,4-linked α-D-Glcp and 1,4-linked β-Galp terminated by α-D-Glcp.					
32871118	6	14	theme	HepG2	1175:1179	arg1	cells					1181:1185	HepG2 cells	1175:1185	HepG2 cells	1175:1185	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
32871118	3	15	theme	1,4-linked	512:521	arg1	residues					548:555	1,4-linked α-d-glucopyranose (Glcp) residues	512:555	1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6	512:587	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	2	16	theme	monosaccharide	301:314	arg1	composition					316:326	Basic monosaccharide composition	295:326	Basic monosaccharide composition	295:326	Basic monosaccharide composition indicated that SSIPS1 was mainly composed of d-glucose.					
32871118	5	17	theme	6.45 nm	898:904	arg1	gyration					877:884	gyration	877:884	gyration of 3.26 and 6.45 nm	877:904	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	5	18	theme	flexible	795:802	arg1	conformation					810:821	a flexible chain conformation	793:821	a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm	793:904	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	2	19	theme	Basic	295:299	arg1	composition					316:326	Basic monosaccharide composition	295:326	Basic monosaccharide composition	295:326	Basic monosaccharide composition indicated that SSIPS1 was mainly composed of d-glucose.					
32871118	6	20	from	effects	1133:1139	arg1	resistance					1161:1170	in vitro insulin resistance	1144:1170	in vitro insulin resistance of HepG2 cells	1144:1185	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
32871118	1	21	theme	cultured	193:200	arg1	mycelia					202:208	cultured mycelia	193:208	cultured mycelia of Sanghuangporus sanghuang	193:236	A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns.					
32871118	3	22	theme	analysis	422:429	arg1	results					388:394	The results	384:394	The results of methylation and 2D-NMR analysis	384:429	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	0	23	from	activity	45:52	arg1	mycelia					119:125	Sanghuangporus sanghuang mycelia	94:125	Sanghuangporus sanghuang mycelia	94:125	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	5	24	theme	0.559 nm	968:975	arg1	chain					959:963	a single chain	950:963	a single chain of 0.559 nm observed by atomic force microscopy	950:1011	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	5	25	theme	multi-angle	909:919	arg1	scattering					933:942	multi-angle laser light scattering	909:942	multi-angle laser light scattering	909:942	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	0	26	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	5	27	from	conformation	810:821	arg1	NaNO3					830:834	0.1 NaNO3	826:834	0.1 NaNO3	826:834	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	3	28	theme	SSIPS1	473:478	arg1	linkages					461:468	the glycosidic linkages	446:468	the glycosidic linkages of SSIPS1	446:478	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	0	29	theme	hypoglycemic	32:43	arg1	activity					45:52	hypoglycemic activity	32:52	hypoglycemic activity	32:52	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	5	30	theme	chain	750:754	arg1	conformation					756:767	its chain conformation	746:767	its chain conformation	746:767	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	1	31	theme	Sanghuangporus	213:226	arg1	sanghuang					228:236	Sanghuangporus sanghuang	213:236	Sanghuangporus sanghuang	213:236	A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns.					
32871118	3	32	theme	2D-NMR	415:420	arg1	analysis					422:429	2D-NMR analysis	415:429	2D-NMR analysis	415:429	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	1	33	theme	neutral	130:136	arg1	SSIPS1					154:159	SSIPS1	154:159	SSIPS1	154:159	A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns.					
32871118	1	33	theme	neutral	130:136	arg1	polysaccharide					138:151	A neutral polysaccharide	128:151	A neutral polysaccharide (SSIPS1)	128:160	A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns.					
32871118	1	34	theme	sanghuang	228:236	arg1	mycelia					202:208	cultured mycelia	193:208	cultured mycelia of Sanghuangporus sanghuang	193:236	A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns.					
32871118	3	35	theme	glycosidic	450:459	arg1	linkages					461:468	the glycosidic linkages	446:468	the glycosidic linkages of SSIPS1	446:478	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	5	36	theme	3.26	889:892	arg1	gyration					877:884	gyration	877:884	gyration of 3.26 and 6.45 nm	877:904	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	5	37	theme	laser	921:925	arg1	scattering					933:942	multi-angle laser light scattering	909:942	multi-angle laser light scattering	909:942	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	4	38	theme	1,4-linked	694:703	arg1	β-Galp					705:710	1,4-linked β-Galp	694:710	1,4-linked β-Galp	694:710	The two branches were composed of 1,4-linked α-D-Glcp terminated with α-D-Glcp, 1,4-linked α-D-Glcp and 1,4-linked β-Galp terminated by α-D-Glcp.					
32871118	6	39	theme	hypoglycemic	1120:1131	arg1	effects					1133:1139	hypoglycemic effects	1120:1139	hypoglycemic effects on in vitro insulin resistance of HepG2 cells	1120:1185	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
32871118	0	40	theme	intracellular	60:72	arg1	polysaccharide					74:87	an intracellular polysaccharide	57:87	an intracellular polysaccharide from Sanghuangporus sanghuang mycelia	57:125	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	3	41	theme	Glcp	542:545	arg1	residues					548:555	1,4-linked α-d-glucopyranose (Glcp) residues	512:555	1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6	512:587	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	5	42	theme	hydrodynamic	843:854	arg1	radius					856:861	a hydrodynamic radius	841:861	a hydrodynamic radius	841:861	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	6	43	dep	in	1144:1145	arg1	vitro					1147:1151	vitro	1147:1151	vitro	1147:1151	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
32871118	5	44	theme	single	952:957	arg1	chain					959:963	a single chain	950:963	a single chain of 0.559 nm observed by atomic force microscopy	950:1011	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	5	45	with	conformation	810:821	arg1	radius					867:872	radius	867:872	radius of gyration of 3.26 and 6.45 nm	867:904	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	5	45	with	conformation	810:821	arg1	radius					856:861	a hydrodynamic radius	841:861	a hydrodynamic radius	841:861	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	4	46	link	1,4-linked	624:633	arg1	α-D-Glcp					635:642	1,4-linked α-D-Glcp	624:642	1,4-linked α-D-Glcp terminated with α-D-Glcp, 1,4-linked α-D-Glcp and 1,4-linked β-Galp terminated by α-D-Glcp	624:733	The two branches were composed of 1,4-linked α-D-Glcp terminated with α-D-Glcp, 1,4-linked α-D-Glcp and 1,4-linked β-Galp terminated by α-D-Glcp.					
32871118	4	47	link	1,4-linked	694:703	arg1	β-Galp					705:710	1,4-linked β-Galp	694:710	1,4-linked β-Galp	694:710	The two branches were composed of 1,4-linked α-D-Glcp terminated with α-D-Glcp, 1,4-linked α-D-Glcp and 1,4-linked β-Galp terminated by α-D-Glcp.					
32871118	3	48	theme	α-d-glucopyranose	523:539	arg1	residues					548:555	1,4-linked α-d-glucopyranose (Glcp) residues	512:555	1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6	512:587	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	6	49	theme	insulin	1153:1159	arg1	resistance					1161:1170	in vitro insulin resistance	1144:1170	in vitro insulin resistance of HepG2 cells	1144:1185	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
32871118	1	50	theme	Fast	256:259	arg1	Flow					261:264	DEAE Sepharose Fast Flow	241:264	DEAE Sepharose Fast Flow	241:264	A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns.					
32871118	5	51	theme	chain	804:808	arg1	conformation					810:821	a flexible chain conformation	793:821	a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm	793:904	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	0	52	from	mycelia	119:125	arg1	polysaccharide					74:87	an intracellular polysaccharide	57:87	an intracellular polysaccharide from Sanghuangporus sanghuang mycelia	57:125	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	0	52	from	mycelia	119:125	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	0	52	from	mycelia	119:125	arg1	activity					45:52	hypoglycemic activity	32:52	hypoglycemic activity	32:52	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	5	53	theme	gyration	877:884	arg1	radius					867:872	radius	867:872	radius of gyration of 3.26 and 6.45 nm	867:904	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	5	53	theme	gyration	877:884	arg1	radius					856:861	a hydrodynamic radius	841:861	a hydrodynamic radius	841:861	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	5	54	theme	light	927:931	arg1	scattering					933:942	multi-angle laser light scattering	909:942	multi-angle laser light scattering	909:942	Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy.					
32871118	6	55	theme	inhibitory	1052:1061	arg1	activity					1063:1070	a potential inhibitory activity	1040:1070	a potential inhibitory activity against α-amylase and α-glucosidase	1040:1106	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
32871118	0	56	theme	polysaccharide	74:87	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	0	56	theme	polysaccharide	74:87	arg1	activity					45:52	hypoglycemic activity	32:52	hypoglycemic activity	32:52	Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia.					
32871118	6	57	theme	cells	1181:1185	arg1	resistance					1161:1170	in vitro insulin resistance	1144:1170	in vitro insulin resistance of HepG2 cells	1144:1185	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
32871118	4	58	link	1,4-linked	670:679	arg1	α-D-Glcp					681:688	1,4-linked α-D-Glcp	670:688	1,4-linked α-D-Glcp	670:688	The two branches were composed of 1,4-linked α-D-Glcp terminated with α-D-Glcp, 1,4-linked α-D-Glcp and 1,4-linked β-Galp terminated by α-D-Glcp.					
32871118	3	59	theme	branched	566:573	arg1	points					575:580	two branched points	562:580	two branched points at O-6	562:587	The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6.					
32871118	6	60	contain	had	1116:1118	arg1	it					1113:1114	it	1113:1114	it	1113:1114	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
32871118	6	60	contain	had	1116:1118	arg2	effects					1133:1139	hypoglycemic effects	1120:1139	hypoglycemic effects on in vitro insulin resistance of HepG2 cells	1120:1185	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
32871118	6	61	theme	potential	1042:1050	arg1	activity					1063:1070	a potential inhibitory activity	1040:1070	a potential inhibitory activity against α-amylase and α-glucosidase	1040:1106	Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.					
33278999	7	0	dep	S	1095:1095	arg1	∼2					1092:1093	∼2	1092:1093	∼2	1092:1093	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	7	0	dep	S	1095:1095	arg1	∼1					1085:1086	∼1	1085:1086	∼1	1085:1086	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	7	1	theme	polysaccharides	1004:1018	arg1	presence					992:999	the presence	988:999	the presence of polysaccharides	988:1018	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	3	2	theme	stem	509:512	arg1	cells					514:518	pluripotent stem cells	497:518	human induced pluripotent stem cells (hiPSC)	483:526	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	3	2	theme	stem	509:512	arg1	hiPSC					521:525	hiPSC	521:525	hiPSC	521:525	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	7	3	dep	cm-1	1097:1100	arg1	S					1095:1095	S	1095:1095	S	1095:1095	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	3	4	from	cytocompatibility	410:426	arg1	terms					431:435	terms	431:435	terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC)	431:526	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	3	5	theme	cells	514:518	arg1	lines					474:478	two lines	470:478	two lines of human induced pluripotent stem cells (hiPSC)	470:526	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	8	6	theme	composites	1457:1466	arg1	application					1405:1415	wide application potential	1400:1425	wide application potential of these polysaccharide-based composites	1400:1466	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.					
33278999	5	7	theme	formed	657:662	arg1	cardiomyocytes					664:677	formed cardiomyocytes	657:677	formed cardiomyocytes	657:677	The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues.					
33278999	8	8	theme	conductivity	1317:1328	arg1	combination					1302:1312	The combination	1298:1312	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC	1298:1392	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.					
33278999	4	9	theme	clusters	590:597	arg1	formation					569:577	the cardiomyogenesis and even formation	539:577	formation	569:577	Moreover, the cardiomyogenesis and even formation of beating clusters were successfully induced on the films.					
33278999	5	10	theme	stimuli-responsive	753:770	arg1	tissues					772:778	stimuli-responsive tissues	753:778	stimuli-responsive tissues	753:778	The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues.					
33278999	5	11	theme	cardiomyocytes	664:677	arg1	proportion					643:652	The proportion	639:652	The proportion of formed cardiomyocytes	639:677	The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues.					
33278999	7	12	theme	surface	1244:1250	arg1	roughness					1252:1260	mean surface roughness	1239:1260	mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH)	1239:1295	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	4	13	theme	beating	582:588	arg1	clusters					590:597	beating clusters	582:597	beating clusters	582:597	Moreover, the cardiomyogenesis and even formation of beating clusters were successfully induced on the films.					
33278999	5	14	theme	tissues	772:778	arg1	engineering					738:748	tissue engineering	731:748	tissue engineering of stimuli-responsive tissues	731:778	The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues.					
33278999	2	15	theme	chitosan	357:364	arg1	presence					318:325	the presence	314:325	the presence of sodium hyaluronate (SH) or chitosan (CH)	314:369	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	6	16	theme	films	841:845	arg1	activity					825:832	antibacterial activity	811:832	antibacterial activity of the films against E. coli	811:861	The testing also demonstrated antibacterial activity of the films against E. coli and PANI-SH was able to reduce bacterial growth from 2 × 105 to < 1 cfu cm-2.					
33278999	8	17	theme	antibacterial	1331:1343	arg1	activity					1345:1352	antibacterial activity	1331:1352	antibacterial activity	1331:1352	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.					
33278999	3	18	theme	adhesion	440:447	arg1	terms					431:435	terms	431:435	terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC)	431:526	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	6	19	dep	1	929:929	arg1	to					924:925	to	924:925	to	924:925	The testing also demonstrated antibacterial activity of the films against E. coli and PANI-SH was able to reduce bacterial growth from 2 × 105 to < 1 cfu cm-2.					
33278999	2	20	theme	composite	150:158	arg1	films					160:164	Novel composite films	144:164	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI)	144:239	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	7	21	theme	Physicochemical	941:955	arg1	characterization					957:972	Physicochemical characterization	941:972	Physicochemical characterization	941:972	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	2	22	theme	hydrochloride	297:309	arg1	polymerization					271:284	the in-situ polymerization	259:284	the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH)	259:369	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	3	23	theme	induced	489:495	arg1	cells					514:518	pluripotent stem cells	497:518	human induced pluripotent stem cells (hiPSC)	483:526	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	3	23	theme	induced	489:495	arg1	hiPSC					521:525	hiPSC	521:525	hiPSC	521:525	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	0	24	theme	composite	11:19	arg1	films					21:25	Conducting composite films	0:25	Conducting composite films	0:25	Conducting composite films based on chitosan or sodium hyaluronate.					
33278999	2	25	theme	Novel	144:148	arg1	films					160:164	Novel composite films	144:164	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI)	144:239	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	6	26	theme	×	918:918	arg1	cm-2					935:938	2 × 105 to < 1 cfu cm-2	916:938	2 × 105 to < 1 cfu cm-2	916:938	The testing also demonstrated antibacterial activity of the films against E. coli and PANI-SH was able to reduce bacterial growth from 2 × 105 to < 1 cfu cm-2.					
33278999	0	27	theme	Conducting	0:9	arg1	films					21:25	Conducting composite films	0:25	Conducting composite films	0:25	Conducting composite films based on chitosan or sodium hyaluronate.					
33278999	4	28	theme	cardiomyogenesis	543:558	arg1	formation					569:577	the cardiomyogenesis and even formation	539:577	formation	569:577	Moreover, the cardiomyogenesis and even formation of beating clusters were successfully induced on the films.					
33278999	3	29	contain	possess	392:398	arg1	films					386:390	The composite films	372:390	The composite films	372:390	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	3	29	contain	possess	392:398	arg2	cytocompatibility					410:426	very good cytocompatibility	400:426	very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC)	400:526	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	2	30	theme	hyaluronate	337:347	arg1	presence					318:325	the presence	314:325	the presence of sodium hyaluronate (SH) or chitosan (CH)	314:369	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	2	31	from	polymerization	271:284	arg1	presence					318:325	the presence	314:325	the presence of sodium hyaluronate (SH) or chitosan (CH)	314:369	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	8	32	theme	polysaccharide-based	1436:1455	arg1	composites					1457:1466	these polysaccharide-based composites	1430:1466	these polysaccharide-based composites	1430:1466	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.					
33278999	1	33	with	cytocompatibility	83:99	arg1	cells					137:141	human induced pluripotent stem cells	106:141	human induced pluripotent stem cells	106:141	Properties and cytocompatibility with human induced pluripotent stem cells.					
33278999	5	34	theme	composites	716:725	arg1	properties					702:711	excellent properties	692:711	excellent properties of composites for tissue engineering of stimuli-responsive tissues	692:778	The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues.					
33278999	6	35	theme	antibacterial	811:823	arg1	activity					825:832	antibacterial activity	811:832	antibacterial activity of the films against E. coli	811:861	The testing also demonstrated antibacterial activity of the films against E. coli and PANI-SH was able to reduce bacterial growth from 2 × 105 to < 1 cfu cm-2.					
33278999	2	36	theme	sodium	330:335	arg1	SH					350:351	SH	350:351	SH	350:351	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	2	36	theme	sodium	330:335	arg1	hyaluronate					337:347	sodium hyaluronate	330:347	sodium hyaluronate (SH)	330:352	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	3	37	theme	composite	376:384	arg1	films					386:390	The composite films	372:390	The composite films	372:390	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	2	38	theme	conducting	211:220	arg1	PANI					235:238	PANI	235:238	PANI	235:238	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	2	38	theme	conducting	211:220	arg1	polyaniline					222:232	conducting polyaniline	211:232	conducting polyaniline (PANI)	211:239	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	1	39	theme	induced	112:118	arg1	cells					137:141	human induced pluripotent stem cells	106:141	human induced pluripotent stem cells	106:141	Properties and cytocompatibility with human induced pluripotent stem cells.					
33278999	3	40	theme	lines	474:478	arg1	adhesion					440:447	adhesion	440:447	adhesion	440:447	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	3	40	theme	lines	474:478	arg1	proliferation					453:465	proliferation	453:465	proliferation	453:465	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	1	41	with	Properties	68:77	arg1	cells					137:141	human induced pluripotent stem cells	106:141	human induced pluripotent stem cells	106:141	Properties and cytocompatibility with human induced pluripotent stem cells.					
33278999	3	42	theme	pluripotent	497:507	arg1	cells					514:518	pluripotent stem cells	497:518	human induced pluripotent stem cells (hiPSC)	483:526	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	3	42	theme	pluripotent	497:507	arg1	hiPSC					521:525	hiPSC	521:525	hiPSC	521:525	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	5	43	theme	tissue	731:736	arg1	engineering					738:748	tissue engineering	731:748	tissue engineering of stimuli-responsive tissues	731:778	The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues.					
33278999	6	44	theme	bacterial	894:902	arg1	growth					904:909	bacterial growth	894:909	bacterial growth	894:909	The testing also demonstrated antibacterial activity of the films against E. coli and PANI-SH was able to reduce bacterial growth from 2 × 105 to < 1 cfu cm-2.					
33278999	3	45	theme	proliferation	453:465	arg1	terms					431:435	terms	431:435	terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC)	431:526	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	7	46	theme	neat	1168:1171	arg1	PANI					1173:1176	neat PANI	1168:1176	neat PANI	1168:1176	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	7	47	theme	nm	1284:1285	arg1	roughness					1252:1260	mean surface roughness	1239:1260	mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH)	1239:1295	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	0	48	theme	sodium	48:53	arg1	hyaluronate					55:65	sodium hyaluronate	48:65	sodium hyaluronate	48:65	Conducting composite films based on chitosan or sodium hyaluronate.					
33278999	2	49	theme	biocompatible	176:188	arg1	polysaccharides					190:204	biocompatible polysaccharides	176:204	biocompatible polysaccharides	176:204	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	8	50	theme	wide	1400:1403	arg1	application					1405:1415	wide application potential	1400:1425	wide application potential of these polysaccharide-based composites	1400:1466	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.					
33278999	8	51	theme	activity	1345:1352	arg1	combination					1302:1312	The combination	1298:1312	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC	1298:1392	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.					
33278999	1	52	theme	pluripotent	120:130	arg1	cells					137:141	human induced pluripotent stem cells	106:141	human induced pluripotent stem cells	106:141	Properties and cytocompatibility with human induced pluripotent stem cells.					
33278999	3	53	theme	good	405:408	arg1	cytocompatibility					410:426	very good cytocompatibility	400:426	very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC)	400:526	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	7	54	with	comparison	1152:1161	arg1	PANI					1173:1176	neat PANI	1168:1176	neat PANI	1168:1176	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	5	55	theme	excellent	692:700	arg1	properties					702:711	excellent properties	692:711	excellent properties of composites for tissue engineering of stimuli-responsive tissues	692:778	The proportion of formed cardiomyocytes demonstrated excellent properties of composites for tissue engineering of stimuli-responsive tissues.					
33278999	8	56	with	combination	1302:1312	arg1	hiPSC					1388:1392	hiPSC	1388:1392	hiPSC	1388:1392	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.					
33278999	1	57	theme	stem	132:135	arg1	cells					137:141	human induced pluripotent stem cells	106:141	human induced pluripotent stem cells	106:141	Properties and cytocompatibility with human induced pluripotent stem cells.					
33278999	7	58	dep	revealed	974:981	arg1	modified					1182:1189	modified	1182:1189	modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH)	1182:1295	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	8	59	theme	potential	1417:1425	arg1	application					1405:1415	wide application potential	1400:1425	wide application potential of these polysaccharide-based composites	1400:1466	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.					
33278999	2	60	theme	aniline	289:295	arg1	hydrochloride					297:309	aniline hydrochloride	289:309	aniline hydrochloride	289:309	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	1	61	theme	human	106:110	arg1	cells					137:141	human induced pluripotent stem cells	106:141	human induced pluripotent stem cells	106:141	Properties and cytocompatibility with human induced pluripotent stem cells.					
33278999	7	62	theme	composites	1068:1077	arg1	conductivities					1046:1059	conductivities	1046:1059	conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH	1046:1124	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	7	63	with	smoother	1225:1232	arg1	roughness					1252:1260	mean surface roughness	1239:1260	mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH)	1239:1295	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	3	64	theme	human	483:487	arg1	cells					514:518	pluripotent stem cells	497:518	human induced pluripotent stem cells (hiPSC)	483:526	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	3	64	theme	human	483:487	arg1	hiPSC					521:525	hiPSC	521:525	hiPSC	521:525	The composite films possess very good cytocompatibility in terms of adhesion and proliferation of two lines of human induced pluripotent stem cells (hiPSC).					
33278999	8	65	theme	cytocompatibility	1365:1381	arg1	combination					1302:1312	The combination	1298:1312	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC	1298:1392	The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites.					
33278999	7	66	theme	mean	1239:1242	arg1	roughness					1252:1260	mean surface roughness	1239:1260	mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH)	1239:1295	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
33278999	6	67	theme	cfu	931:933	arg1	cm-2					935:938	2 × 105 to < 1 cfu cm-2	916:938	2 × 105 to < 1 cfu cm-2	916:938	The testing also demonstrated antibacterial activity of the films against E. coli and PANI-SH was able to reduce bacterial growth from 2 × 105 to < 1 cfu cm-2.					
33278999	2	68	theme	in-situ	263:269	arg1	polymerization					271:284	the in-situ polymerization	259:284	the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH)	259:369	Novel composite films combining biocompatible polysaccharides with conducting polyaniline (PANI) were prepared via the in-situ polymerization of aniline hydrochloride in the presence of sodium hyaluronate (SH) or chitosan (CH).					
33278999	7	69	theme	4	1265:1265	arg1	roughness					1252:1260	mean surface roughness	1239:1260	mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH)	1239:1295	Physicochemical characterization revealed that the presence of polysaccharides did not notably influence conductivities of the composites being ∼1 and ∼2 S cm-1 for PANI-SH and PANI-CH respectively; however, in comparison with neat PANI, it modified their topography making the films smoother with mean surface roughness of 4 (PANI-SH) and 14 nm (PANI-CH).					
31941812	9	0	from	fungi	1947:1951	arg1	galactomannans					1917:1930	galactomannans	1917:1930	galactomannans in filamentous fungi	1917:1951	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	9	0	from	fungi	1947:1951	arg1	residues					1905:1912	β-(1→5)-galactofuranosyl residues	1880:1912	β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi	1880:1951	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	9	1	theme	glycan	2050:2055	arg1	biosynthesis					2029:2040	biosynthesis	2029:2040	biosynthesis of this glycan	2029:2055	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	13	2	theme	cell	2665:2668	arg1	structure					2675:2683	the complex cell wall structure	2653:2683	the complex cell wall structure	2653:2683	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	7	3	theme	limited	1331:1337	arg1	function					1339:1346	limited function	1331:1346	limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues	1331:1408	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	3	4	theme	β-	471:472	arg1	-galactofuranosyltransferase					478:505	a β-galactofuranoside β-(1→5)-galactofuranosyltransferase	449:505	a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains	449:569	We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains.					
31941812	3	4	theme	β-	471:472	arg1	GfsA					441:444	GfsA	441:444	GfsA	441:444	We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains.					
31941812	7	5	theme	antifungal	1609:1618	arg1	agents					1620:1625	antifungal agents	1609:1625	antifungal agents	1609:1625	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	12	6	theme	-galactofuranosyl	2414:2430	arg1	residues					2432:2439	β-(1→5)-galactofuranosyl residues	2407:2439	β-(1→5)-galactofuranosyl residues	2407:2439	The data presented here indicate that β-(1→5)-galactofuranosyl residues are involved in cell growth, conidiation, polarity, and cell surface hydrophobicity.					
31941812	8	7	theme	many	1821:1824	arg1	pathogens					1843:1851	many plant and animal pathogens	1821:1851	many plant and animal pathogens	1821:1851	Pezizomycotina includes many plant and animal pathogens.					
31941812	5	8	theme	β-galactofuranoside	776:794	arg1	GfsB					736:739	GfsB	736:739	GfsB	736:739	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	5	8	theme	β-galactofuranoside	776:794	arg1	-galactofuranosyltransferases					803:831	β-galactofuranoside β-(1→5)-galactofuranosyltransferases	776:831	β-galactofuranoside β-(1→5)-galactofuranosyltransferases	776:831	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	5	8	theme	β-galactofuranoside	776:794	arg1	GfsC					745:748	GfsC	745:748	GfsC	745:748	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	7	9	theme	O-mannose-type	1277:1290	arg1	galactomannans					1292:1305	fungal-type and O-mannose-type galactomannans	1261:1305	fungal-type and O-mannose-type galactomannans	1261:1305	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	10	10	theme	formation	2075:2083	arg1	processes					2085:2093	Fungal cell wall formation processes	2058:2093	Fungal cell wall formation processes	2058:2093	Fungal cell wall formation processes are complicated, and information concerning glycosyltransferases is essential for understanding them.					
31941812	0	11	theme	Pathogen	117:124	arg1	fumigatus					138:146	the Invasive Pathogen Aspergillus fumigatus	104:146	the Invasive Pathogen Aspergillus fumigatus	104:146	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.					
31941812	13	12	theme	novel	2616:2620	arg1	insights					2622:2629	important novel insights	2606:2629	important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina	2606:2748	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	7	13	theme	cell	1532:1535	arg1	hydrophobicity					1545:1558	cell surface hydrophobicity	1532:1558	cell surface hydrophobicity	1532:1558	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	14	theme	fungal-type	1261:1271	arg1	galactomannans					1292:1305	fungal-type and O-mannose-type galactomannans	1261:1305	fungal-type and O-mannose-type galactomannans	1261:1305	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	10	15	theme	cell	2065:2068	arg1	processes					2085:2093	Fungal cell wall formation processes	2058:2093	Fungal cell wall formation processes	2058:2093	Fungal cell wall formation processes are complicated, and information concerning glycosyltransferases is essential for understanding them.					
31941812	1	16	located	localized	217:225	arg2	galactomannans					202:215	galactomannans	202:215	galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes	202:316	The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes.					
31941812	1	16	located	localized	217:225	arg1	layer					242:246	the surface layer	230:246	the surface layer	230:246	The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes.					
31941812	3	17	theme	chains	564:569	arg1	biosynthesis					523:534	the biosynthesis	519:534	the biosynthesis of β-(1→5)-galactofuranosyl chains	519:569	We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains.					
31941812	7	18	theme	immunocompromised	1648:1664	arg1	residues					1707:1714	immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues	1648:1714	immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota	1648:1794	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	19	theme	hyphal	1501:1506	arg1	structure					1518:1526	the abnormal hyphal branching structure	1488:1526	the abnormal hyphal branching structure	1488:1526	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	9	20	theme	residues	1905:1912	arg1	structure					1867:1875	the structure	1863:1875	the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi	1863:1951	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	6	21	theme	activity	1075:1082	arg1	assay					1038:1042	an established in vitro highly efficient assay	997:1042	an established in vitro highly efficient assay of galactofuranosyltransferase activity	997:1082	GfsA, GfsB, and GfsC can synthesize β-(1→5)-galactofuranosyl oligomers at up to lengths of 7, 3, and 5 galactofuranoses within an established in vitro highly efficient assay of galactofuranosyltransferase activity.					
31941812	9	22	theme	galactomannans	1917:1930	arg1	galactomannans					1917:1930	galactomannans	1917:1930	galactomannans in filamentous fungi	1917:1951	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	9	22	theme	galactomannans	1917:1930	arg1	residues					1905:1912	β-(1→5)-galactofuranosyl residues	1880:1912	β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi	1880:1951	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	13	23	theme	-galactofuranosyl	2558:2574	arg1	biosynthesis					2584:2595	β-(1→5)-galactofuranosyl residue biosynthesis	2551:2595	β-(1→5)-galactofuranosyl residue biosynthesis	2551:2595	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	9	24	theme	filamentous	1935:1945	arg1	fungi					1947:1951	filamentous fungi	1935:1951	filamentous fungi	1935:1951	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	4	25	theme	chains	645:650	arg1	biosynthesis					604:615	the biosynthesis	600:615	the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC	600:720	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	13	26	theme	biosynthesis	2584:2595	arg1	understanding					2534:2546	Our new understanding	2526:2546	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis	2526:2595	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	7	27	theme	formation	1456:1464	arg1	ability					1466:1472	conidium formation ability	1447:1472	conidium formation ability	1447:1472	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	11	28	theme	residues	2310:2317	arg1	biosynthesis					2265:2276	the biosynthesis	2261:2276	the biosynthesis of all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans	2261:2366	In this study, we showed that GfsA and GfsC are responsible for the biosynthesis of all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans.					
31941812	0	29	theme	Galactomannans	82:95	arg1	Chains					41:46	β-(1→5)-Galactofuranosyl Chains	16:46	β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans	16:95	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.					
31941812	13	30	theme	Pezizomycotina	2735:2748	arg1	members					2710:2716	the members	2706:2716	the members of the subphylum Pezizomycotina	2706:2748	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	7	31	theme	Structural	1085:1094	arg1	analyses					1096:1103	Structural analyses	1085:1103	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains	1085:1178	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	32	theme	β-	1224:1225	arg1	galactomannans					1292:1305	fungal-type and O-mannose-type galactomannans	1261:1305	fungal-type and O-mannose-type galactomannans	1261:1305	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	32	theme	β-	1224:1225	arg1	residues					1249:1256	all β-(1→5)-galactofuranosyl residues	1220:1256	all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans	1220:1305	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	11	33	theme	O-mannose-type	2338:2351	arg1	galactomannans					2353:2366	fungal-type and O-mannose-type galactomannans	2322:2366	fungal-type and O-mannose-type galactomannans	2322:2366	In this study, we showed that GfsA and GfsC are responsible for the biosynthesis of all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans.					
31941812	2	34	theme	β-	371:372	arg1	chains					405:410	β-(1→5)-/β-(1→6)-galactofuranosyl chains	371:410	β-(1→5)-/β-(1→6)-galactofuranosyl chains	371:410	Galactomannans comprise α-(1→2)-/α-(1→6)-mannan and β-(1→5)-/β-(1→6)-galactofuranosyl chains.					
31941812	13	35	theme	members	2710:2716	arg1	formation					2640:2648	the formation	2636:2648	the formation of the complex cell wall structure	2636:2683	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	13	35	theme	members	2710:2716	arg1	virulence					2693:2701	the virulence	2689:2701	the virulence of the members of the subphylum Pezizomycotina	2689:2748	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	9	36	from	galactomannans	1917:1930	arg1	fungi					1947:1951	filamentous fungi	1935:1951	filamentous fungi	1935:1951	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	2	37	theme	-galactofuranosyl	387:403	arg1	chains					405:410	β-(1→5)-/β-(1→6)-galactofuranosyl chains	371:410	β-(1→5)-/β-(1→6)-galactofuranosyl chains	371:410	Galactomannans comprise α-(1→2)-/α-(1→6)-mannan and β-(1→5)-/β-(1→6)-galactofuranosyl chains.					
31941812	11	38	theme	fungal-type	2322:2332	arg1	galactomannans					2353:2366	fungal-type and O-mannose-type galactomannans	2322:2366	fungal-type and O-mannose-type galactomannans	2322:2366	In this study, we showed that GfsA and GfsC are responsible for the biosynthesis of all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans.					
31941812	0	39	theme	β-	16:17	arg1	Chains					41:46	β-(1→5)-Galactofuranosyl Chains	16:46	β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans	16:95	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.					
31941812	1	40	theme	biological	297:306	arg1	processes					308:316	various biological processes	289:316	various biological processes	289:316	The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes.					
31941812	11	41	theme	galactomannans	2353:2366	arg1	galactomannans					2353:2366	fungal-type and O-mannose-type galactomannans	2322:2366	fungal-type and O-mannose-type galactomannans	2322:2366	In this study, we showed that GfsA and GfsC are responsible for the biosynthesis of all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans.					
31941812	11	41	theme	galactomannans	2353:2366	arg1	residues					2310:2317	all β-(1→5)-galactofuranosyl residues	2281:2317	all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans	2281:2366	In this study, we showed that GfsA and GfsC are responsible for the biosynthesis of all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans.					
31941812	7	42	theme	hyphal	1424:1429	arg1	rate					1438:1441	the hyphal growth rate	1420:1441	the hyphal growth rate	1420:1441	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	9	43	from	residues	1905:1912	arg1	fungi					1947:1951	filamentous fungi	1935:1951	filamentous fungi	1935:1951	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	2	44	theme	-/β-	378:381	arg1	chains					405:410	β-(1→5)-/β-(1→6)-galactofuranosyl chains	371:410	β-(1→5)-/β-(1→6)-galactofuranosyl chains	371:410	Galactomannans comprise α-(1→2)-/α-(1→6)-mannan and β-(1→5)-/β-(1→6)-galactofuranosyl chains.					
31941812	0	45	theme	1→5	19:21	arg1	Chains					41:46	β-(1→5)-Galactofuranosyl Chains	16:46	β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans	16:95	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.					
31941812	2	46	theme	-/α-	350:353	arg1	-mannan					359:365	α-(1→2)-/α-(1→6)-mannan	343:365	α-(1→2)-/α-(1→6)-mannan	343:365	Galactomannans comprise α-(1→2)-/α-(1→6)-mannan and β-(1→5)-/β-(1→6)-galactofuranosyl chains.					
31941812	0	47	theme	-Galactofuranosyl	23:39	arg1	Chains					41:46	β-(1→5)-Galactofuranosyl Chains	16:46	β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans	16:95	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.					
31941812	7	48	theme	-galactofuranosyl	1383:1399	arg1	residues					1401:1408	β-(1→5)-galactofuranosyl residues	1376:1408	β-(1→5)-galactofuranosyl residues	1376:1408	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	4	49	from	fumigatus	658:666	arg1	biosynthesis					604:615	the biosynthesis	600:615	the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC	600:720	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	6	50	theme	galactofuranoses	973:988	arg1	lengths					950:956	lengths	950:956	lengths of 7, 3, and 5 galactofuranoses within an established in vitro highly efficient assay of galactofuranosyltransferase activity	950:1082	GfsA, GfsB, and GfsC can synthesize β-(1→5)-galactofuranosyl oligomers at up to lengths of 7, 3, and 5 galactofuranoses within an established in vitro highly efficient assay of galactofuranosyltransferase activity.					
31941812	12	51	theme	surface	2502:2508	arg1	hydrophobicity					2510:2523	cell surface hydrophobicity	2497:2523	cell surface hydrophobicity	2497:2523	The data presented here indicate that β-(1→5)-galactofuranosyl residues are involved in cell growth, conidiation, polarity, and cell surface hydrophobicity.					
31941812	3	52	theme	1→5	474:476	arg1	-galactofuranosyltransferase					478:505	a β-galactofuranoside β-(1→5)-galactofuranosyltransferase	449:505	a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains	449:569	We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains.					
31941812	3	52	theme	1→5	474:476	arg1	GfsA					441:444	GfsA	441:444	GfsA	441:444	We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains.					
31941812	1	53	theme	cell	255:258	arg1	walls					260:264	its cell walls	251:264	its cell walls	251:264	The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes.					
31941812	12	54	theme	cell	2457:2460	arg1	growth					2462:2467	cell growth	2457:2467	cell growth	2457:2467	The data presented here indicate that β-(1→5)-galactofuranosyl residues are involved in cell growth, conidiation, polarity, and cell surface hydrophobicity.					
31941812	7	55	theme	galactomannans	1108:1121	arg1	analyses					1096:1103	Structural analyses	1085:1103	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains	1085:1178	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	2	56	theme	1→6	355:357	arg1	-mannan					359:365	α-(1→2)-/α-(1→6)-mannan	343:365	α-(1→2)-/α-(1→6)-mannan	343:365	Galactomannans comprise α-(1→2)-/α-(1→6)-mannan and β-(1→5)-/β-(1→6)-galactofuranosyl chains.					
31941812	13	57	theme	complex	2657:2663	arg1	structure					2675:2683	the complex cell wall structure	2653:2683	the complex cell wall structure	2653:2683	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	13	58	theme	residue	2576:2582	arg1	biosynthesis					2584:2595	β-(1→5)-galactofuranosyl residue biosynthesis	2551:2595	β-(1→5)-galactofuranosyl residue biosynthesis	2551:2595	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	13	59	theme	wall	2670:2673	arg1	structure					2675:2683	the complex cell wall structure	2653:2683	the complex cell wall structure	2653:2683	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	7	60	theme	-Galactofuranosyl	1689:1705	arg1	residues					1707:1714	immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues	1648:1714	immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota	1648:1794	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	6	61	theme	galactofuranosyltransferase	1047:1073	arg1	activity					1075:1082	galactofuranosyltransferase activity	1047:1082	galactofuranosyltransferase activity	1047:1082	GfsA, GfsB, and GfsC can synthesize β-(1→5)-galactofuranosyl oligomers at up to lengths of 7, 3, and 5 galactofuranoses within an established in vitro highly efficient assay of galactofuranosyltransferase activity.					
31941812	10	62	theme	Fungal	2058:2063	arg1	processes					2085:2093	Fungal cell wall formation processes	2058:2093	Fungal cell wall formation processes	2058:2093	Fungal cell wall formation processes are complicated, and information concerning glycosyltransferases is essential for understanding them.					
31941812	7	63	theme	ΔgfsABC	1164:1170	arg1	strains					1172:1178	ΔgfsABC strains	1164:1178	ΔgfsABC strains	1164:1178	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	8	64	theme	plant	1826:1830	arg1	pathogens					1843:1851	many plant and animal pathogens	1821:1851	many plant and animal pathogens	1821:1851	Pezizomycotina includes many plant and animal pathogens.					
31941812	7	65	theme	1→5	1685:1687	arg1	residues					1707:1714	immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues	1648:1714	immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota	1648:1794	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	6	66	dep	in	1012:1013	arg1	efficient					1028:1036	efficient	1028:1036	efficient	1028:1036	GfsA, GfsB, and GfsC can synthesize β-(1→5)-galactofuranosyl oligomers at up to lengths of 7, 3, and 5 galactofuranoses within an established in vitro highly efficient assay of galactofuranosyltransferase activity.					
31941812	6	66	dep	in	1012:1013	arg1	vitro					1015:1019	vitro	1015:1019	vitro	1015:1019	GfsA, GfsB, and GfsC can synthesize β-(1→5)-galactofuranosyl oligomers at up to lengths of 7, 3, and 5 galactofuranoses within an established in vitro highly efficient assay of galactofuranosyltransferase activity.					
31941812	8	67	theme	animal	1836:1841	arg1	pathogens					1843:1851	many plant and animal pathogens	1821:1851	many plant and animal pathogens	1821:1851	Pezizomycotina includes many plant and animal pathogens.					
31941812	4	68	from	biosynthesis	604:615	arg1	fumigatus					658:666	A. fumigatus	655:666	A. fumigatus	655:666	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	7	69	theme	surface	1537:1543	arg1	hydrophobicity					1545:1558	cell surface hydrophobicity	1532:1558	cell surface hydrophobicity	1532:1558	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	70	theme	β-	1682:1683	arg1	residues					1707:1714	immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues	1648:1714	immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota	1648:1794	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	4	71	theme	β-	620:621	arg1	GfsC					717:720	GfsC	717:720	GfsC	717:720	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	4	71	theme	β-	620:621	arg1	chains					645:650	β-(1→5)-galactofuranosyl chains	620:650	β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC	620:720	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	4	71	theme	β-	620:621	arg1	GfsB					708:711	GfsB	708:711	GfsB	708:711	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	0	72	theme	Aspergillus	126:136	arg1	fumigatus					138:146	the Invasive Pathogen Aspergillus fumigatus	104:146	the Invasive Pathogen Aspergillus fumigatus	104:146	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.					
31941812	13	73	theme	important	2606:2614	arg1	insights					2622:2629	important novel insights	2606:2629	important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina	2606:2748	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	7	74	theme	galactomannans	1292:1305	arg1	galactomannans					1292:1305	fungal-type and O-mannose-type galactomannans	1261:1305	fungal-type and O-mannose-type galactomannans	1261:1305	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	74	theme	galactomannans	1292:1305	arg1	residues					1249:1256	all β-(1→5)-galactofuranosyl residues	1220:1256	all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans	1220:1305	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	10	75	theme	wall	2070:2073	arg1	processes					2085:2093	Fungal cell wall formation processes	2058:2093	Fungal cell wall formation processes	2058:2093	Fungal cell wall formation processes are complicated, and information concerning glycosyltransferases is essential for understanding them.					
31941812	3	76	theme	-galactofuranosyl	546:562	arg1	chains					564:569	β-(1→5)-galactofuranosyl chains	539:569	β-(1→5)-galactofuranosyl chains	539:569	We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains.					
31941812	1	77	theme	surface	234:240	arg1	layer					242:246	the surface layer	230:246	the surface layer	230:246	The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes.					
31941812	7	78	theme	branching	1508:1516	arg1	structure					1518:1526	the abnormal hyphal branching structure	1488:1526	the abnormal hyphal branching structure	1488:1526	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	79	theme	abnormal	1492:1499	arg1	structure					1518:1526	the abnormal hyphal branching structure	1488:1526	the abnormal hyphal branching structure	1488:1526	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	9	80	theme	-galactofuranosyl	1887:1903	arg1	galactomannans					1917:1930	galactomannans	1917:1930	galactomannans in filamentous fungi	1917:1951	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	9	80	theme	-galactofuranosyl	1887:1903	arg1	residues					1905:1912	β-(1→5)-galactofuranosyl residues	1880:1912	β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi	1880:1951	Although the structure of β-(1→5)-galactofuranosyl residues of galactomannans in filamentous fungi was discovered long ago, it remains unclear which enzyme is responsible for biosynthesis of this glycan.					
31941812	5	81	theme	biochemical	836:846	arg1	analyses					860:867	biochemical and genetic analyses	836:867	biochemical and genetic analyses	836:867	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	7	82	theme	mice.IMPORTANCE	1666:1680	arg1	residues					1707:1714	immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues	1648:1714	immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota	1648:1794	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	6	83	theme	-galactofuranosyl	913:929	arg1	oligomers					931:939	β-(1→5)-galactofuranosyl oligomers	906:939	β-(1→5)-galactofuranosyl oligomers	906:939	GfsA, GfsB, and GfsC can synthesize β-(1→5)-galactofuranosyl oligomers at up to lengths of 7, 3, and 5 galactofuranoses within an established in vitro highly efficient assay of galactofuranosyltransferase activity.					
31941812	7	84	theme	-galactofuranosyl	1231:1247	arg1	galactomannans					1292:1305	fungal-type and O-mannose-type galactomannans	1261:1305	fungal-type and O-mannose-type galactomannans	1261:1305	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	84	theme	-galactofuranosyl	1231:1247	arg1	residues					1249:1256	all β-(1→5)-galactofuranosyl residues	1220:1256	all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans	1220:1305	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	12	85	theme	β-	2407:2408	arg1	residues					2432:2439	β-(1→5)-galactofuranosyl residues	2407:2439	β-(1→5)-galactofuranosyl residues	2407:2439	The data presented here indicate that β-(1→5)-galactofuranosyl residues are involved in cell growth, conidiation, polarity, and cell surface hydrophobicity.					
31941812	0	86	theme	Fungal-Type	51:61	arg1	Galactomannans					82:95	Fungal-Type and O-Mannose-Type Galactomannans	51:95	Fungal-Type and O-Mannose-Type Galactomannans	51:95	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.					
31941812	7	87	from	function	1339:1346	arg1	A.					1351:1352	A.	1351:1352	A.	1351:1352	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	5	88	theme	β-	796:797	arg1	GfsB					736:739	GfsB	736:739	GfsB	736:739	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	5	88	theme	β-	796:797	arg1	-galactofuranosyltransferases					803:831	β-galactofuranoside β-(1→5)-galactofuranosyltransferases	776:831	β-galactofuranoside β-(1→5)-galactofuranosyltransferases	776:831	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	5	88	theme	β-	796:797	arg1	GfsC					745:748	GfsC	745:748	GfsC	745:748	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	7	89	theme	1→5	1227:1229	arg1	galactomannans					1292:1305	fungal-type and O-mannose-type galactomannans	1261:1305	fungal-type and O-mannose-type galactomannans	1261:1305	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	89	theme	1→5	1227:1229	arg1	residues					1249:1256	all β-(1→5)-galactofuranosyl residues	1220:1256	all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans	1220:1305	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	4	90	theme	1→5	623:625	arg1	GfsC					717:720	GfsC	717:720	GfsC	717:720	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	4	90	theme	1→5	623:625	arg1	chains					645:650	β-(1→5)-galactofuranosyl chains	620:650	β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC	620:720	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	4	90	theme	1→5	623:625	arg1	GfsB					708:711	GfsB	708:711	GfsB	708:711	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	0	91	theme	O-Mannose-Type	67:80	arg1	Galactomannans					82:95	Fungal-Type and O-Mannose-Type Galactomannans	51:95	Fungal-Type and O-Mannose-Type Galactomannans	51:95	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.					
31941812	5	92	theme	1→5	799:801	arg1	GfsB					736:739	GfsB	736:739	GfsB	736:739	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	5	92	theme	1→5	799:801	arg1	-galactofuranosyltransferases					803:831	β-galactofuranoside β-(1→5)-galactofuranosyltransferases	776:831	β-galactofuranoside β-(1→5)-galactofuranosyltransferases	776:831	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	5	92	theme	1→5	799:801	arg1	GfsC					745:748	GfsC	745:748	GfsC	745:748	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	7	93	theme	conidium	1447:1454	arg1	ability					1466:1472	conidium formation ability	1447:1472	conidium formation ability	1447:1472	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	11	94	theme	-galactofuranosyl	2292:2308	arg1	galactomannans					2353:2366	fungal-type and O-mannose-type galactomannans	2322:2366	fungal-type and O-mannose-type galactomannans	2322:2366	In this study, we showed that GfsA and GfsC are responsible for the biosynthesis of all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans.					
31941812	11	94	theme	-galactofuranosyl	2292:2308	arg1	residues					2310:2317	all β-(1→5)-galactofuranosyl residues	2281:2317	all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans	2281:2366	In this study, we showed that GfsA and GfsC are responsible for the biosynthesis of all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans.					
31941812	4	95	theme	-galactofuranosyl	627:643	arg1	GfsC					717:720	GfsC	717:720	GfsC	717:720	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	4	95	theme	-galactofuranosyl	627:643	arg1	chains					645:650	β-(1→5)-galactofuranosyl chains	620:650	β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC	620:720	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	4	95	theme	-galactofuranosyl	627:643	arg1	GfsB					708:711	GfsB	708:711	GfsB	708:711	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	4	96	dep	chains	645:650	arg1	GfsC					717:720	GfsC	717:720	GfsC	717:720	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	4	96	dep	chains	645:650	arg1	chains					645:650	β-(1→5)-galactofuranosyl chains	620:650	β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC	620:720	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	4	96	dep	chains	645:650	arg1	GfsB					708:711	GfsB	708:711	GfsB	708:711	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	4	96	dep	chains	645:650	arg1	exist					681:685	exist	681:685	exist within A. fumigatus	681:705	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	6	97	theme	in	1012:1013	arg1	assay					1038:1042	an established in vitro highly efficient assay	997:1042	an established in vitro highly efficient assay of galactofuranosyltransferase activity	997:1082	GfsA, GfsB, and GfsC can synthesize β-(1→5)-galactofuranosyl oligomers at up to lengths of 7, 3, and 5 galactofuranoses within an established in vitro highly efficient assay of galactofuranosyltransferase activity.					
31941812	0	98	theme	Invasive	108:115	arg1	fumigatus					138:146	the Invasive Pathogen Aspergillus fumigatus	104:146	the Invasive Pathogen Aspergillus fumigatus	104:146	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.					
31941812	12	99	theme	cell	2497:2500	arg1	hydrophobicity					2510:2523	cell surface hydrophobicity	2497:2523	cell surface hydrophobicity	2497:2523	The data presented here indicate that β-(1→5)-galactofuranosyl residues are involved in cell growth, conidiation, polarity, and cell surface hydrophobicity.					
31941812	5	100	theme	genetic	852:858	arg1	analyses					860:867	biochemical and genetic analyses	836:867	biochemical and genetic analyses	836:867	We show that GfsB and GfsC, in addition to GfsA, are β-galactofuranoside β-(1→5)-galactofuranosyltransferases by biochemical and genetic analyses.					
31941812	13	101	theme	new	2530:2532	arg1	understanding					2534:2546	Our new understanding	2526:2546	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis	2526:2595	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	1	102	theme	various	289:295	arg1	processes					308:316	various biological processes	289:316	various biological processes	289:316	The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes.					
31941812	12	103	theme	1→5	2410:2412	arg1	residues					2432:2439	β-(1→5)-galactofuranosyl residues	2407:2439	β-(1→5)-galactofuranosyl residues	2407:2439	The data presented here indicate that β-(1→5)-galactofuranosyl residues are involved in cell growth, conidiation, polarity, and cell surface hydrophobicity.					
31941812	7	104	theme	growth	1431:1436	arg1	rate					1438:1441	the hyphal growth rate	1420:1441	the hyphal growth rate	1420:1441	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	1	105	contain	contains	193:200	arg1	fumigatus					183:191	The pathogenic fungus Aspergillus fumigatus	149:191	The pathogenic fungus Aspergillus fumigatus	149:191	The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes.					
31941812	1	105	contain	contains	193:200	arg2	galactomannans					202:215	galactomannans	202:215	galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes	202:316	The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes.					
31941812	0	106	theme	Chains	41:46	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.	0:147	Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus.					
31941812	7	107	dep	A.	1351:1352	arg1	fumigatus					1354:1362	A. fumigatus The loss	1351:1371	A. fumigatus The loss	1351:1371	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	107	dep	A.	1351:1352	arg1	loss					1368:1371	The loss	1364:1371	A. fumigatus The loss	1351:1371	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	108	theme	phylum	1778:1783	arg1	Ascomycota					1785:1794	the phylum Ascomycota	1774:1794	the phylum Ascomycota	1774:1794	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	6	109	theme	established	1000:1010	arg1	assay					1038:1042	an established in vitro highly efficient assay	997:1042	an established in vitro highly efficient assay of galactofuranosyltransferase activity	997:1082	GfsA, GfsB, and GfsC can synthesize β-(1→5)-galactofuranosyl oligomers at up to lengths of 7, 3, and 5 galactofuranoses within an established in vitro highly efficient assay of galactofuranosyltransferase activity.					
31941812	3	110	theme	β-galactofuranoside	451:469	arg1	-galactofuranosyltransferase					478:505	a β-galactofuranoside β-(1→5)-galactofuranosyltransferase	449:505	a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains	449:569	We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains.					
31941812	3	110	theme	β-galactofuranoside	451:469	arg1	GfsA					441:444	GfsA	441:444	GfsA	441:444	We previously revealed that GfsA is a β-galactofuranoside β-(1→5)-galactofuranosyltransferase involved in the biosynthesis of β-(1→5)-galactofuranosyl chains.					
31941812	13	111	theme	structure	2675:2683	arg1	formation					2640:2648	the formation	2636:2648	the formation of the complex cell wall structure	2636:2683	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	13	111	theme	structure	2675:2683	arg1	virulence					2693:2701	the virulence	2689:2701	the virulence of the members of the subphylum Pezizomycotina	2689:2748	Our new understanding of β-(1→5)-galactofuranosyl residue biosynthesis provides important novel insights into the formation of the complex cell wall structure and the virulence of the members of the subphylum Pezizomycotina.					
31941812	7	112	theme	residues	1401:1408	arg1	function					1339:1346	limited function	1331:1346	limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues	1331:1408	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	7	113	dep	residues	1707:1714	arg1	distributed					1727:1737	distributed	1727:1737	are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota	1716:1794	Structural analyses of galactomannans extracted from ΔgfsB, ΔgfsC, ΔgfsAC, and ΔgfsABC strains revealed that GfsA and GfsC synthesized all β-(1→5)-galactofuranosyl residues of fungal-type and O-mannose-type galactomannans and that GfsB exhibited limited function in A. fumigatus The loss of β-(1→5)-galactofuranosyl residues decreased the hyphal growth rate and conidium formation ability and increased the abnormal hyphal branching structure and cell surface hydrophobicity, but this loss is dispensable for sensitivity to antifungal agents and virulence toward immunocompromised mice.IMPORTANCE β-(1→5)-Galactofuranosyl residues are widely distributed in the subphylum Pezizomycotina of the phylum Ascomycota.					
31941812	4	114	from	chains	645:650	arg1	fumigatus					658:666	A. fumigatus	655:666	A. fumigatus	655:666	In this study, we clarified the biosynthesis of β-(1→5)-galactofuranosyl chains in A. fumigatus Two paralogs exist within A. fumigatus: GfsB and GfsC.					
31941812	1	115	theme	walls	260:264	arg1	layer					242:246	the surface layer	230:246	the surface layer	230:246	The pathogenic fungus Aspergillus fumigatus contains galactomannans localized on the surface layer of its cell walls, which are involved in various biological processes.					
33932420	4	0	theme	DSC	831:833	arg1	analysis					835:842	DSC analysis	831:842	DSC analysis	831:842	The morphological features of novel hydrogel were perceived by FTIR, XRD, FESEM, and DSC analysis.					
33932420	3	1	theme	factor	684:689	arg1	CS-U-CNC-EGF					703:714	CS-U-CNC-EGF	703:714	CS-U-CNC-EGF	703:714	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	3	1	theme	factor	684:689	arg1	drug					697:700	epidermal growth factor (EGF) drug	667:700	epidermal growth factor (EGF) drug (CS-U-CNC-EGF)	667:715	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	5	2	from	size	934:937	arg1	microstructure					911:924	the porous microstructure	900:924	the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	900:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	1	3	theme	natural	240:246	arg1	combinations					262:273	their natural and synthetic combinations	234:273	their natural and synthetic combinations like; microfibers, film, nanofibers, hydrogels, and various drugs	234:339	Several dressing materials can be used efficiently in recent times, both in their natural and synthetic combinations like; microfibers, film, nanofibers, hydrogels, and various drugs.					
33932420	9	4	theme	hydrogel	1617:1624	arg1	applications					1586:1597	possible future applications	1570:1597	possible future applications of this composite hydrogel in wound healing	1570:1641	The study's results point to possible future applications of this composite hydrogel in wound healing as a wound dressing material.					
33932420	6	5	theme	excellent	1118:1126	arg1	proliferation					1133:1145	excellent cell proliferation	1118:1145	excellent cell proliferation	1118:1145	The novel nanocomposites revealed non-toxic behavior and excellent cell proliferation.					
33932420	1	6	theme	synthetic	252:260	arg1	combinations					262:273	their natural and synthetic combinations	234:273	their natural and synthetic combinations like; microfibers, film, nanofibers, hydrogels, and various drugs	234:339	Several dressing materials can be used efficiently in recent times, both in their natural and synthetic combinations like; microfibers, film, nanofibers, hydrogels, and various drugs.					
33932420	9	7	theme	wound	1629:1633	arg1	healing					1635:1641	wound healing	1629:1641	wound healing	1629:1641	The study's results point to possible future applications of this composite hydrogel in wound healing as a wound dressing material.					
33932420	5	8	dep	μm	967:968	arg1	to					956:957	to	956:957	to	956:957	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	7	9	theme	treated	1319:1325	arg1	group					1327:1331	a 100% wound contraction treated group	1294:1331	a 100% wound contraction treated group	1294:1331	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	3	10	theme	freeze-dried	724:735	arg1	process					737:743	the freeze-dried process	720:743	the freeze-dried process	720:743	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	5	11	from	μm	967:968	arg1	microstructure					911:924	the porous microstructure	900:924	the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	900:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	9	12	theme	wound	1648:1652	arg1	material					1663:1670	a wound dressing material	1646:1670	a wound dressing material	1646:1670	The study's results point to possible future applications of this composite hydrogel in wound healing as a wound dressing material.					
33932420	6	13	theme	novel	1065:1069	arg1	nanocomposites					1071:1084	The novel nanocomposites	1061:1084	The novel nanocomposites	1061:1084	The novel nanocomposites revealed non-toxic behavior and excellent cell proliferation.					
33932420	0	14	theme	In	127:128	arg1	analysis					148:155	In vitro and in vivo analysis	127:155	In vitro and in vivo analysis	127:155	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	9	15	from	healing	1635:1641	arg1	applications					1586:1597	possible future applications	1570:1597	possible future applications of this composite hydrogel in wound healing	1570:1641	The study's results point to possible future applications of this composite hydrogel in wound healing as a wound dressing material.					
33932420	8	16	theme	controlled	1348:1357	arg1	release					1359:1365	the controlled release	1344:1365	the controlled release of EGF from CS-U-CNC-EGF hydrogels	1344:1400	Moreover, the controlled release of EGF from CS-U-CNC-EGF hydrogels showed significantly faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition.					
33932420	7	17	theme	100	1296:1298	arg1	%					1299:1299	%	1299:1299	%	1299:1299	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	3	18	theme	novel	583:587	arg1	hydrogel					604:611	novel chitosan-ulvan hydrogel	583:611	novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process	583:743	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	1	19	theme	recent	212:217	arg1	times					219:223	recent times	212:223	recent times	212:223	Several dressing materials can be used efficiently in recent times, both in their natural and synthetic combinations like; microfibers, film, nanofibers, hydrogels, and various drugs.					
33932420	5	20	theme	improved	971:978	arg1	curve					998:1002	improved mechanical stress curve	971:1002	improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	971:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	20	theme	improved	971:978	arg1	μm					967:968	237 ± 59 μm to 53 ± 16 μm	944:968	237 ± 59 μm to 53 ± 16 μm	944:968	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	21	theme	content	883:889	arg1	incorporation					849:861	The incorporation	845:861	The incorporation of the nanocrystals content	845:889	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	22	theme	stress	991:996	arg1	curve					998:1002	improved mechanical stress curve	971:1002	improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	971:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	22	theme	stress	991:996	arg1	μm					967:968	237 ± 59 μm to 53 ± 16 μm	944:968	237 ± 59 μm to 53 ± 16 μm	944:968	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	0	23	theme	epidermal	75:83	arg1	factor					92:97	epidermal growth factor	75:97	epidermal growth factor for enhanced wound healing	75:124	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	0	24	theme	hydrogel	21:28	arg1	reinforcement					30:42	Novel chitosan-ulvan hydrogel reinforcement	0:42	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing	0:124	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	0	25	dep	In	127:128	arg1	vitro					130:134	vitro	130:134	vitro	130:134	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	8	26	theme	healing	1436:1442	arg1	efficiency					1444:1453	faster-wound healing efficiency	1423:1453	faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition	1423:1538	Moreover, the controlled release of EGF from CS-U-CNC-EGF hydrogels showed significantly faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition.					
33932420	0	27	theme	enhanced	103:110	arg1	healing					118:124	enhanced wound healing	103:124	enhanced wound healing	103:124	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	5	28	theme	1.2	1021:1023	arg1	MPa					1014:1016	MPa	1014:1016	MPa	1014:1016	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	9	29	from	applications	1586:1597	arg1	healing					1635:1641	wound healing	1629:1641	wound healing	1629:1641	The study's results point to possible future applications of this composite hydrogel in wound healing as a wound dressing material.					
33932420	5	30	theme	±	948:948	arg1	curve					998:1002	improved mechanical stress curve	971:1002	improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	971:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	30	theme	±	948:948	arg1	μm					967:968	237 ± 59 μm to 53 ± 16 μm	944:968	237 ± 59 μm to 53 ± 16 μm	944:968	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	7	31	theme	wound	1266:1270	arg1	site					1272:1275	the wound site	1262:1275	the wound site for 15 days from a 100% wound contraction treated group	1262:1331	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	8	32	theme	faster	1479:1484	arg1	formation					1506:1514	considerably faster granulations tissue formation	1466:1514	considerably faster granulations tissue formation	1466:1514	Moreover, the controlled release of EGF from CS-U-CNC-EGF hydrogels showed significantly faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition.					
33932420	0	33	theme	growth	85:90	arg1	factor					92:97	epidermal growth factor	75:97	epidermal growth factor for enhanced wound healing	75:124	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	0	34	theme	Novel	0:4	arg1	reinforcement					30:42	Novel chitosan-ulvan hydrogel reinforcement	0:42	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing	0:124	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	2	35	theme	favorable	413:421	arg1	environment					423:433	a favorable environment	411:433	providing a favorable environment for wound healing	401:451	The specific characteristics, such as biocompatibility and providing a favorable environment for wound healing, make many polysaccharides pivotal as wound dressings.					
33932420	8	36	theme	tissue	1499:1504	arg1	formation					1506:1514	considerably faster granulations tissue formation	1466:1514	considerably faster granulations tissue formation	1466:1514	Moreover, the controlled release of EGF from CS-U-CNC-EGF hydrogels showed significantly faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition.					
33932420	2	37	theme	providing	401:409	arg1	environment					423:433	a favorable environment	411:433	providing a favorable environment for wound healing	401:451	The specific characteristics, such as biocompatibility and providing a favorable environment for wound healing, make many polysaccharides pivotal as wound dressings.					
33932420	5	38	from	MPa	1025:1027	arg1	curve					998:1002	improved mechanical stress curve	971:1002	improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	971:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	38	from	MPa	1025:1027	arg1	μm					967:968	237 ± 59 μm to 53 ± 16 μm	944:968	237 ± 59 μm to 53 ± 16 μm	944:968	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	39	theme	pore	929:932	arg1	size					934:937	pore size	929:937	pore size	929:937	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	7	40	theme	EGF	1246:1248	arg1	delivery					1250:1257	EGF delivery	1246:1257	EGF delivery	1246:1257	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	3	41	theme	growth	677:682	arg1	EGF					692:694	EGF	692:694	EGF	692:694	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	3	41	theme	growth	677:682	arg1	factor					684:689	epidermal growth factor	667:689	epidermal growth factor (EGF) drug (CS-U-CNC-EGF)	667:715	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	4	42	theme	novel	776:780	arg1	hydrogel					782:789	novel hydrogel	776:789	novel hydrogel	776:789	The morphological features of novel hydrogel were perceived by FTIR, XRD, FESEM, and DSC analysis.					
33932420	5	43	theme	μm	953:954	arg1	curve					998:1002	improved mechanical stress curve	971:1002	improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	971:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	43	theme	μm	953:954	arg1	μm					967:968	237 ± 59 μm to 53 ± 16 μm	944:968	237 ± 59 μm to 53 ± 16 μm	944:968	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	7	44	theme	growth	1207:1212	arg1	EGF					1222:1224	EGF	1222:1224	EGF	1222:1224	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	7	44	theme	growth	1207:1212	arg1	factor					1214:1219	the epidermal growth factor	1193:1219	the epidermal growth factor (EGF)	1193:1225	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	9	45	theme	composite	1607:1615	arg1	hydrogel					1617:1624	this composite hydrogel	1602:1624	this composite hydrogel in wound healing	1602:1641	The study's results point to possible future applications of this composite hydrogel in wound healing as a wound dressing material.					
33932420	1	46	theme	dressing	166:173	arg1	materials					175:183	Several dressing materials	158:183	Several dressing materials	158:183	Several dressing materials can be used efficiently in recent times, both in their natural and synthetic combinations like; microfibers, film, nanofibers, hydrogels, and various drugs.					
33932420	6	47	theme	non-toxic	1095:1103	arg1	behavior					1105:1112	non-toxic behavior	1095:1112	non-toxic behavior	1095:1112	The novel nanocomposites revealed non-toxic behavior and excellent cell proliferation.					
33932420	3	48	theme	polysaccharides	548:562	arg1	importance					528:537	the importance	524:537	the importance of these polysaccharides	524:562	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	9	49	theme	dressing	1654:1661	arg1	material					1663:1670	a wound dressing material	1646:1670	a wound dressing material	1646:1670	The study's results point to possible future applications of this composite hydrogel in wound healing as a wound dressing material.					
33932420	1	50	theme	Several	158:164	arg1	materials					175:183	Several dressing materials	158:183	Several dressing materials	158:183	Several dressing materials can be used efficiently in recent times, both in their natural and synthetic combinations like; microfibers, film, nanofibers, hydrogels, and various drugs.					
33932420	0	51	theme	in	140:141	arg1	analysis					148:155	In vitro and in vivo analysis	127:155	In vitro and in vivo analysis	127:155	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	5	52	theme	0.57	1009:1012	arg1	MPa					1014:1016	MPa	1014:1016	MPa	1014:1016	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	9	53	from	hydrogel	1617:1624	arg1	healing					1635:1641	wound healing	1629:1641	wound healing	1629:1641	The study's results point to possible future applications of this composite hydrogel in wound healing as a wound dressing material.					
33932420	1	54	dep	times	219:223	arg1	both					226:229	both	226:229	both	226:229	Several dressing materials can be used efficiently in recent times, both in their natural and synthetic combinations like; microfibers, film, nanofibers, hydrogels, and various drugs.					
33932420	7	55	theme	%	1299:1299	arg1	contraction					1307:1317	a 100% wound contraction	1294:1317	a 100% wound contraction treated group	1294:1331	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	5	56	theme	nanocrystals	870:881	arg1	content					883:889	the nanocrystals content	866:889	the nanocrystals content	866:889	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	8	57	theme	CS-U-CNC-EGF	1379:1390	arg1	hydrogels					1392:1400	CS-U-CNC-EGF hydrogels	1379:1400	CS-U-CNC-EGF hydrogels	1379:1400	Moreover, the controlled release of EGF from CS-U-CNC-EGF hydrogels showed significantly faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition.					
33932420	1	58	used	used	192:195	arg2	materials					175:183	Several dressing materials	158:183	Several dressing materials	158:183	Several dressing materials can be used efficiently in recent times, both in their natural and synthetic combinations like; microfibers, film, nanofibers, hydrogels, and various drugs.					
33932420	5	59	theme	mechanical	980:989	arg1	curve					998:1002	improved mechanical stress curve	971:1002	improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	971:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	59	theme	mechanical	980:989	arg1	μm					967:968	237 ± 59 μm to 53 ± 16 μm	944:968	237 ± 59 μm to 53 ± 16 μm	944:968	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	0	60	dep	analysis	148:155	arg1	reinforcement					30:42	Novel chitosan-ulvan hydrogel reinforcement	0:42	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing	0:124	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	7	61	theme	wound	1301:1305	arg1	contraction					1307:1317	a 100% wound contraction	1294:1317	a 100% wound contraction treated group	1294:1331	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	7	62	theme	sustained	1172:1180	arg1	release					1182:1188	sustained release	1172:1188	sustained release of the epidermal growth factor (EGF)	1172:1225	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	3	63	theme	chitosan-ulvan	589:602	arg1	hydrogel					604:611	novel chitosan-ulvan hydrogel	583:611	novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process	583:743	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	0	64	dep	in	140:141	arg1	vivo					143:146	vivo	143:146	vivo	143:146	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	7	65	from	group	1327:1331	arg1	days					1284:1287	15 days	1281:1287	15 days from a 100% wound contraction treated group	1281:1331	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	8	66	theme	faster-wound	1423:1434	arg1	efficiency					1444:1453	faster-wound healing efficiency	1423:1453	faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition	1423:1538	Moreover, the controlled release of EGF from CS-U-CNC-EGF hydrogels showed significantly faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition.					
33932420	6	67	theme	cell	1128:1131	arg1	proliferation					1133:1145	excellent cell proliferation	1118:1145	excellent cell proliferation	1118:1145	The novel nanocomposites revealed non-toxic behavior and excellent cell proliferation.					
33932420	8	68	from	hydrogels	1392:1400	arg1	release					1359:1365	the controlled release	1344:1365	the controlled release of EGF from CS-U-CNC-EGF hydrogels	1344:1400	Moreover, the controlled release of EGF from CS-U-CNC-EGF hydrogels showed significantly faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition.					
33932420	5	69	theme	swelling	1042:1049	arg1	behavior					1051:1058	thermal and swelling behavior	1030:1058	thermal and swelling behavior	1030:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	69	theme	swelling	1042:1049	arg1	MPa					1025:1027	0.57 MPa to 1.2 MPa	1009:1027	0.57 MPa to 1.2 MPa	1009:1027	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	1	70	theme	various	327:333	arg1	drugs					335:339	various drugs	327:339	various drugs	327:339	Several dressing materials can be used efficiently in recent times, both in their natural and synthetic combinations like; microfibers, film, nanofibers, hydrogels, and various drugs.					
33932420	2	71	theme	many	459:462	arg1	polysaccharides					464:478	many polysaccharides	459:478	many polysaccharides pivotal as wound dressings	459:505	The specific characteristics, such as biocompatibility and providing a favorable environment for wound healing, make many polysaccharides pivotal as wound dressings.					
33932420	0	72	theme	wound	112:116	arg1	healing					118:124	enhanced wound healing	103:124	enhanced wound healing	103:124	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	9	73	theme	future	1579:1584	arg1	applications					1586:1597	possible future applications	1570:1597	possible future applications of this composite hydrogel in wound healing	1570:1641	The study's results point to possible future applications of this composite hydrogel in wound healing as a wound dressing material.					
33932420	7	74	theme	contraction	1307:1317	arg1	group					1327:1331	a 100% wound contraction treated group	1294:1331	a 100% wound contraction treated group	1294:1331	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	5	75	theme	237	944:946	arg1	curve					998:1002	improved mechanical stress curve	971:1002	improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	971:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	75	theme	237	944:946	arg1	μm					967:968	237 ± 59 μm to 53 ± 16 μm	944:968	237 ± 59 μm to 53 ± 16 μm	944:968	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	2	76	theme	wound	491:495	arg1	dressings					497:505	wound dressings	491:505	wound dressings	491:505	The specific characteristics, such as biocompatibility and providing a favorable environment for wound healing, make many polysaccharides pivotal as wound dressings.					
33932420	8	77	theme	granulations	1486:1497	arg1	formation					1506:1514	considerably faster granulations tissue formation	1466:1514	considerably faster granulations tissue formation	1466:1514	Moreover, the controlled release of EGF from CS-U-CNC-EGF hydrogels showed significantly faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition.					
33932420	4	78	theme	morphological	750:762	arg1	features					764:771	The morphological features	746:771	The morphological features of novel hydrogel	746:789	The morphological features of novel hydrogel were perceived by FTIR, XRD, FESEM, and DSC analysis.					
33932420	0	79	theme	chitosan-ulvan	6:19	arg1	reinforcement					30:42	Novel chitosan-ulvan hydrogel reinforcement	0:42	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing	0:124	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	5	80	theme	thermal	1030:1036	arg1	behavior					1051:1058	thermal and swelling behavior	1030:1058	thermal and swelling behavior	1030:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	80	theme	thermal	1030:1036	arg1	MPa					1025:1027	0.57 MPa to 1.2 MPa	1009:1027	0.57 MPa to 1.2 MPa	1009:1027	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	81	theme	porous	904:909	arg1	microstructure					911:924	the porous microstructure	900:924	the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	900:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	2	82	theme	specific	346:353	arg1	environment					423:433	a favorable environment	411:433	providing a favorable environment for wound healing	401:451	The specific characteristics, such as biocompatibility and providing a favorable environment for wound healing, make many polysaccharides pivotal as wound dressings.					
33932420	2	82	theme	specific	346:353	arg1	biocompatibility					380:395	biocompatibility	380:395	biocompatibility	380:395	The specific characteristics, such as biocompatibility and providing a favorable environment for wound healing, make many polysaccharides pivotal as wound dressings.					
33932420	2	82	theme	specific	346:353	arg1	characteristics					355:369	The specific characteristics	342:369	The specific characteristics	342:369	The specific characteristics, such as biocompatibility and providing a favorable environment for wound healing, make many polysaccharides pivotal as wound dressings.					
33932420	8	83	theme	collagen	1520:1527	arg1	deposition					1529:1538	collagen deposition	1520:1538	collagen deposition	1520:1538	Moreover, the controlled release of EGF from CS-U-CNC-EGF hydrogels showed significantly faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition.					
33932420	7	84	dep	showed	1165:1170	arg1	Whereas					1148:1154	Whereas	1148:1154	Whereas	1148:1154	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	0	85	theme	cellulose	47:55	arg1	nanocrystals					57:68	cellulose nanocrystals	47:68	cellulose nanocrystals	47:68	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	5	86	theme	±	962:962	arg1	curve					998:1002	improved mechanical stress curve	971:1002	improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior	971:1058	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	5	86	theme	±	962:962	arg1	μm					967:968	237 ± 59 μm to 53 ± 16 μm	944:968	237 ± 59 μm to 53 ± 16 μm	944:968	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	3	87	theme	cellulose	629:637	arg1	CNCs					653:656	CNCs	653:656	CNCs	653:656	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	3	87	theme	cellulose	629:637	arg1	nanocrystals					639:650	cellulose nanocrystals	629:650	cellulose nanocrystals (CNCs)	629:657	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	0	88	with	reinforcement	30:42	arg1	factor					92:97	epidermal growth factor	75:97	epidermal growth factor for enhanced wound healing	75:124	Novel chitosan-ulvan hydrogel reinforcement by cellulose nanocrystals with epidermal growth factor for enhanced wound healing: In vitro and in vivo analysis.					
33932420	2	89	theme	wound	439:443	arg1	healing					445:451	wound healing	439:451	wound healing	439:451	The specific characteristics, such as biocompatibility and providing a favorable environment for wound healing, make many polysaccharides pivotal as wound dressings.					
33932420	3	90	theme	epidermal	667:675	arg1	EGF					692:694	EGF	692:694	EGF	692:694	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	3	90	theme	epidermal	667:675	arg1	factor					684:689	epidermal growth factor	667:689	epidermal growth factor (EGF) drug (CS-U-CNC-EGF)	667:715	Keeping in view the importance of these polysaccharides, we have developed novel chitosan-ulvan hydrogel incorporated by cellulose nanocrystals (CNCs) loading epidermal growth factor (EGF) drug (CS-U-CNC-EGF) by the freeze-dried process.					
33932420	8	91	theme	EGF	1370:1372	arg1	release					1359:1365	the controlled release	1344:1365	the controlled release of EGF from CS-U-CNC-EGF hydrogels	1344:1400	Moreover, the controlled release of EGF from CS-U-CNC-EGF hydrogels showed significantly faster-wound healing efficiency concerning considerably faster granulations tissue formation and collagen deposition.					
33932420	9	92	theme	possible	1570:1577	arg1	applications					1586:1597	possible future applications	1570:1597	possible future applications of this composite hydrogel in wound healing	1570:1641	The study's results point to possible future applications of this composite hydrogel in wound healing as a wound dressing material.					
33932420	7	93	theme	factor	1214:1219	arg1	release					1182:1188	sustained release	1172:1188	sustained release of the epidermal growth factor (EGF)	1172:1225	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	5	94	dep	MPa	1014:1016	arg1	to					1018:1019	to	1018:1019	to	1018:1019	The incorporation of the nanocrystals content modified the porous microstructure at pore size from 237 ± 59 μm to 53 ± 16 μm, improved mechanical stress curve from 0.57 MPa to 1.2 MPa, thermal and swelling behavior.					
33932420	7	95	theme	epidermal	1197:1205	arg1	EGF					1222:1224	EGF	1222:1224	EGF	1222:1224	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	7	95	theme	epidermal	1197:1205	arg1	factor					1214:1219	the epidermal growth factor	1193:1219	the epidermal growth factor (EGF)	1193:1225	Whereas hydrogel showed sustained release of the epidermal growth factor (EGF), thereby enhancing EGF delivery at the wound site for 15 days from a 100% wound contraction treated group.					
33932420	4	96	theme	hydrogel	782:789	arg1	features					764:771	The morphological features	746:771	The morphological features of novel hydrogel	746:789	The morphological features of novel hydrogel were perceived by FTIR, XRD, FESEM, and DSC analysis.					
33118591	4	0	theme	58.43	639:643	arg1	%					644:644	%	644:644	%	644:644	In the presence of TS, the encapsulation efficiency of resveratrol was significantly elevated from 58.43% to 85.58%.					
33118591	11	1	theme	complexes	1681:1689	arg1	applications					1638:1649	applications	1638:1649	applications	1638:1649	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	11	1	theme	complexes	1681:1689	arg1	properties					1623:1632	properties	1623:1632	properties	1623:1632	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	6	2	theme	secondary	877:885	arg1	structure					887:895	the micro-environment and secondary structure	851:895	the micro-environment and secondary structure of the protein	851:910	Multiple spectroscopic methods revealed that TS altered the micro-environment and secondary structure of the protein.					
33118591	7	3	theme	hydrophobic	929:939	arg1	effects					941:947	hydrophobic effects	929:947	hydrophobic effects	929:947	Hydrogen bonds, hydrophobic effects and electrostatic interactions contributed to the formation of complex nanoparticles.					
33118591	9	4	theme	scanning	1188:1195	arg1	microscopy					1206:1215	Field emission scanning electron microscopy	1173:1215	Field emission scanning electron microscopy (FE-SEM)	1173:1224	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	9	4	theme	scanning	1188:1195	arg1	FE-SEM					1218:1223	FE-SEM	1218:1223	FE-SEM	1218:1223	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	8	5	theme	X-ray	1079:1083	arg1	XRD					1098:1100	XRD	1098:1100	XRD	1098:1100	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	8	5	theme	X-ray	1079:1083	arg1	diffraction					1085:1095	X-ray diffraction	1079:1095	X-ray diffraction (XRD)	1079:1101	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	6	6	theme	micro-environment	855:871	arg1	structure					887:895	the micro-environment and secondary structure	851:895	the micro-environment and secondary structure of the protein	851:910	Multiple spectroscopic methods revealed that TS altered the micro-environment and secondary structure of the protein.					
33118591	1	7	theme	-tea	209:212	arg1	complex					235:241	novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex	166:241	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol.					
33118591	5	8	theme	resveratrol	688:698	arg1	stability					675:683	The environmental stability	657:683	The environmental stability of resveratrol	657:698	The environmental stability of resveratrol was improved through entrapping into the complex nanoparticles with the rise in TS proportion.					
33118591	11	9	theme	TS	1613:1614	arg1	influence					1600:1608	the influence	1596:1608	the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour	1596:1823	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	2	10	theme	functional	339:348	arg1	digestion					422:430	gastrointestinal digestion	405:430	gastrointestinal digestion of the complex nanoparticles	405:459	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	2	10	theme	functional	339:348	arg1	microstructure					362:375	microstructure	362:375	microstructure	362:375	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	2	10	theme	functional	339:348	arg1	interactions					388:399	molecular interactions	378:399	molecular interactions	378:399	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	2	10	theme	functional	339:348	arg1	attributes					350:359	the functional attributes	335:359	the functional attributes	335:359	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	1	11	theme	complex	235:241	arg1	nanoparticles					243:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol.					
33118591	1	12	theme	zein-propylene	172:185	arg1	complex					235:241	novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex	166:241	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol.					
33118591	11	13	dep	properties	1623:1632	arg1	the					1619:1621	the	1619:1621	the	1619:1621	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	11	14	theme	digestion	1805:1813	arg1	behaviour					1815:1823	digestion behaviour	1805:1823	digestion behaviour	1805:1823	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	1	15	theme	alginate	194:201	arg1	complex					235:241	novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex	166:241	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol.					
33118591	0	16	theme	physicochemical	112:126	arg1	stability					128:136	physicochemical stability	112:136	physicochemical stability	112:136	The construction of resveratrol-loaded protein-polysaccharide-tea saponin complex nanoparticles for controlling physicochemical stability and in vitro digestion.					
33118591	1	17	theme	PGA	204:206	arg1	complex					235:241	novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex	166:241	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol.					
33118591	9	18	theme	different	1286:1294	arg1	states					1308:1313	their different aggregation states	1280:1313	their different aggregation states	1280:1313	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	8	19	theme	amorphous	1123:1131	arg1	nature					1133:1138	the amorphous nature	1119:1138	the amorphous nature of the encapsulated resveratrol	1119:1170	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	9	20	theme	Field	1173:1177	arg1	microscopy					1206:1215	Field emission scanning electron microscopy	1173:1215	Field emission scanning electron microscopy (FE-SEM)	1173:1224	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	9	20	theme	Field	1173:1177	arg1	FE-SEM					1218:1223	FE-SEM	1218:1223	FE-SEM	1218:1223	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	2	21	theme	nanoparticles	447:459	arg1	attributes					350:359	the functional attributes	335:359	the functional attributes	335:359	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	2	21	theme	nanoparticles	447:459	arg1	microstructure					362:375	microstructure	362:375	microstructure	362:375	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	2	21	theme	nanoparticles	447:459	arg1	interactions					388:399	molecular interactions	378:399	molecular interactions	378:399	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	2	21	theme	nanoparticles	447:459	arg1	digestion					422:430	gastrointestinal digestion	405:430	gastrointestinal digestion of the complex nanoparticles	405:459	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	10	22	theme	complex	1386:1392	arg1	nanoparticles					1394:1406	the zein-PGA-TS complex nanoparticles	1370:1406	the zein-PGA-TS complex nanoparticles	1370:1406	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	3	23	theme	zein-PGA-TS	474:484	arg1	nanoparticles					494:506	zein-PGA-TS complex nanoparticles	474:506	zein-PGA-TS complex nanoparticles	474:506	The size of zein-PGA-TS complex nanoparticles was between 281.9 and 309.7 nm.					
33118591	8	24	theme	encapsulated	1147:1158	arg1	resveratrol					1160:1170	the encapsulated resveratrol	1143:1170	the encapsulated resveratrol	1143:1170	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	7	25	theme	complex	1012:1018	arg1	nanoparticles					1020:1032	complex nanoparticles	1012:1032	complex nanoparticles	1012:1032	Hydrogen bonds, hydrophobic effects and electrostatic interactions contributed to the formation of complex nanoparticles.					
33118591	4	26	theme	resveratrol	595:605	arg1	efficiency					581:590	the encapsulation efficiency	563:590	the encapsulation efficiency of resveratrol	563:605	In the presence of TS, the encapsulation efficiency of resveratrol was significantly elevated from 58.43% to 85.58%.					
33118591	9	27	theme	aggregation	1296:1306	arg1	states					1308:1313	their different aggregation states	1280:1313	their different aggregation states	1280:1313	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	11	28	theme	nanoparticles	1762:1774	arg1	development					1730:1740	the development	1726:1740	the development of novel food grade nanoparticles with desirable stability and digestion behaviour	1726:1823	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	0	29	theme	protein-polysaccharide-tea	39:64	arg1	complex					74:80	resveratrol-loaded protein-polysaccharide-tea saponin complex	20:80	resveratrol-loaded protein-polysaccharide-tea saponin complex	20:80	The construction of resveratrol-loaded protein-polysaccharide-tea saponin complex nanoparticles for controlling physicochemical stability and in vitro digestion.					
33118591	11	30	theme	food	1751:1754	arg1	nanoparticles					1762:1774	novel food grade nanoparticles	1745:1774	novel food grade nanoparticles with desirable stability and digestion behaviour	1745:1823	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	2	31	theme	molecular	378:386	arg1	interactions					388:399	molecular interactions	378:399	molecular interactions	378:399	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	2	31	theme	molecular	378:386	arg1	attributes					350:359	the functional attributes	335:359	the functional attributes	335:359	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	0	32	theme	complex	74:80	arg1	construction					4:15	The construction	0:15	The construction of resveratrol-loaded protein-polysaccharide-tea saponin complex	0:80	The construction of resveratrol-loaded protein-polysaccharide-tea saponin complex nanoparticles for controlling physicochemical stability and in vitro digestion.					
33118591	8	33	theme	Differential	1035:1046	arg1	calorimetry					1057:1067	Differential scanning calorimetry	1035:1067	Differential scanning calorimetry (DSC)	1035:1073	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	8	33	theme	Differential	1035:1046	arg1	DSC					1070:1072	DSC	1070:1072	DSC	1070:1072	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	9	34	theme	states	1308:1313	arg1	shape					1249:1253	the globular shape	1236:1253	the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions	1236:1357	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	6	35	theme	spectroscopic	804:816	arg1	methods					818:824	Multiple spectroscopic methods	795:824	Multiple spectroscopic methods	795:824	Multiple spectroscopic methods revealed that TS altered the micro-environment and secondary structure of the protein.					
33118591	6	36	theme	protein	904:910	arg1	structure					887:895	the micro-environment and secondary structure	851:895	the micro-environment and secondary structure of the protein	851:910	Multiple spectroscopic methods revealed that TS altered the micro-environment and secondary structure of the protein.					
33118591	8	37	theme	calorimetry	1057:1067	arg1	patterns					1103:1110	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns	1035:1110	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns	1035:1110	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	4	38	theme	85.58	649:653	arg1	%					644:644	%	644:644	%	644:644	In the presence of TS, the encapsulation efficiency of resveratrol was significantly elevated from 58.43% to 85.58%.					
33118591	11	39	theme	desirable	1781:1789	arg1	stability					1791:1799	desirable stability	1781:1799	desirable stability	1781:1799	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	10	40	dep	 1	1509:1510	arg1	%					1518:1518	23.20%	1513:1518	23.20% in the stomach	1513:1533	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	10	40	dep	 1	1509:1510	arg1	%					1544:1544	63.11%	1539:1544	63.11% in the small intestine	1539:1567	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	9	41	theme	nanoparticles	1262:1274	arg1	shape					1249:1253	the globular shape	1236:1253	the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions	1236:1357	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	5	42	from	rise	772:775	arg1	proportion					783:792	TS proportion	780:792	TS proportion	780:792	The environmental stability of resveratrol was improved through entrapping into the complex nanoparticles with the rise in TS proportion.					
33118591	7	43	theme	electrostatic	953:965	arg1	interactions					967:978	electrostatic interactions	953:978	electrostatic interactions	953:978	Hydrogen bonds, hydrophobic effects and electrostatic interactions contributed to the formation of complex nanoparticles.					
33118591	8	44	theme	diffraction	1085:1095	arg1	patterns					1103:1110	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns	1035:1110	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns	1035:1110	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	5	45	theme	environmental	661:673	arg1	stability					675:683	The environmental stability	657:683	The environmental stability of resveratrol	657:698	The environmental stability of resveratrol was improved through entrapping into the complex nanoparticles with the rise in TS proportion.					
33118591	10	46	theme	zein	1491:1494	arg1	ratio					1482:1486	the mass ratio	1473:1486	the mass ratio of zein to TS	1473:1500	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	4	47	dep	%	644:644	arg1	to					646:647	to	646:647	to	646:647	In the presence of TS, the encapsulation efficiency of resveratrol was significantly elevated from 58.43% to 85.58%.					
33118591	1	48	theme	saponin	214:220	arg1	complex					235:241	novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex	166:241	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol.					
33118591	10	49	from	%	1518:1518	arg1	intestine					1559:1567	the small intestine	1549:1567	the small intestine	1549:1567	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	10	49	from	%	1518:1518	arg1	stomach					1527:1533	the stomach	1523:1533	the stomach	1523:1533	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	7	50	theme	Hydrogen	913:920	arg1	bonds					922:926	Hydrogen bonds	913:926	Hydrogen bonds	913:926	Hydrogen bonds, hydrophobic effects and electrostatic interactions contributed to the formation of complex nanoparticles.					
33118591	1	51	theme	TS	223:224	arg1	complex					235:241	novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex	166:241	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol.					
33118591	9	52	theme	particle	1337:1344	arg1	compositions					1346:1357	the particle compositions	1333:1357	the particle compositions	1333:1357	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	11	53	with	nanoparticles	1762:1774	arg1	stability					1791:1799	desirable stability	1781:1799	desirable stability	1781:1799	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	11	53	with	nanoparticles	1762:1774	arg1	behaviour					1815:1823	digestion behaviour	1805:1823	digestion behaviour	1805:1823	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	1	54	theme	ternary	227:233	arg1	complex					235:241	novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex	166:241	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol.					
33118591	5	55	theme	TS	780:781	arg1	proportion					783:792	TS proportion	780:792	TS proportion	780:792	The environmental stability of resveratrol was improved through entrapping into the complex nanoparticles with the rise in TS proportion.					
33118591	10	56	from	%	1544:1544	arg1	intestine					1559:1567	the small intestine	1549:1567	the small intestine	1549:1567	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	10	56	from	%	1544:1544	arg1	stomach					1527:1533	the stomach	1523:1533	the stomach	1523:1533	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	5	57	theme	complex	741:747	arg1	nanoparticles					749:761	the complex nanoparticles	737:761	the complex nanoparticles	737:761	The environmental stability of resveratrol was improved through entrapping into the complex nanoparticles with the rise in TS proportion.					
33118591	1	58	theme	novel	166:170	arg1	complex					235:241	novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex	166:241	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol.					
33118591	9	59	theme	globular	1240:1247	arg1	shape					1249:1253	the globular shape	1236:1253	the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions	1236:1357	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	1	60	theme	glycol	187:192	arg1	complex					235:241	novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex	166:241	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles	162:255	The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol.					
33118591	10	61	from	release	1437:1443	arg1	intestine					1458:1466	the small intestine	1448:1466	the small intestine	1448:1466	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	10	62	theme	zein-PGA-TS	1374:1384	arg1	nanoparticles					1394:1406	the zein-PGA-TS complex nanoparticles	1370:1406	the zein-PGA-TS complex nanoparticles	1370:1406	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	10	63	dep	exhibited	1408:1416	arg1	 1					1509:1510	 1	1509:1510	 1	1509:1510	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	11	64	from	influence	1600:1608	arg1	applications					1638:1649	applications	1638:1649	applications	1638:1649	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	11	64	from	influence	1600:1608	arg1	properties					1623:1632	properties	1623:1632	properties	1623:1632	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	0	65	theme	in	142:143	arg1	digestion					151:159	in vitro digestion	142:159	in vitro digestion	142:159	The construction of resveratrol-loaded protein-polysaccharide-tea saponin complex nanoparticles for controlling physicochemical stability and in vitro digestion.					
33118591	0	66	dep	in	142:143	arg1	vitro					145:149	vitro	145:149	vitro	145:149	The construction of resveratrol-loaded protein-polysaccharide-tea saponin complex nanoparticles for controlling physicochemical stability and in vitro digestion.					
33118591	9	67	theme	emission	1179:1186	arg1	microscopy					1206:1215	Field emission scanning electron microscopy	1173:1215	Field emission scanning electron microscopy (FE-SEM)	1173:1224	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	9	67	theme	emission	1179:1186	arg1	FE-SEM					1218:1223	FE-SEM	1218:1223	FE-SEM	1218:1223	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	2	68	theme	complex	439:445	arg1	nanoparticles					447:459	the complex nanoparticles	435:459	the complex nanoparticles	435:459	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	8	69	theme	resveratrol	1160:1170	arg1	nature					1133:1138	the amorphous nature	1119:1138	the amorphous nature of the encapsulated resveratrol	1119:1170	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	9	70	theme	electron	1197:1204	arg1	microscopy					1206:1215	Field emission scanning electron microscopy	1173:1215	Field emission scanning electron microscopy (FE-SEM)	1173:1224	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	9	70	theme	electron	1197:1204	arg1	FE-SEM					1218:1223	FE-SEM	1218:1223	FE-SEM	1218:1223	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	3	71	theme	complex	486:492	arg1	nanoparticles					494:506	zein-PGA-TS complex nanoparticles	474:506	zein-PGA-TS complex nanoparticles	474:506	The size of zein-PGA-TS complex nanoparticles was between 281.9 and 309.7 nm.					
33118591	0	72	theme	resveratrol-loaded	20:37	arg1	complex					74:80	resveratrol-loaded protein-polysaccharide-tea saponin complex	20:80	resveratrol-loaded protein-polysaccharide-tea saponin complex	20:80	The construction of resveratrol-loaded protein-polysaccharide-tea saponin complex nanoparticles for controlling physicochemical stability and in vitro digestion.					
33118591	7	73	theme	nanoparticles	1020:1032	arg1	formation					999:1007	the formation	995:1007	the formation of complex nanoparticles	995:1032	Hydrogen bonds, hydrophobic effects and electrostatic interactions contributed to the formation of complex nanoparticles.					
33118591	9	74	dep	nanoparticles	1262:1274	arg1	the					1258:1260	the	1258:1260	the	1258:1260	Field emission scanning electron microscopy (FE-SEM) confirmed the globular shape of the nanoparticles and their different aggregation states were dependent on the particle compositions.					
33118591	10	75	theme	small	1553:1557	arg1	intestine					1559:1567	the small intestine	1549:1567	the small intestine	1549:1567	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	11	76	theme	grade	1756:1760	arg1	nanoparticles					1762:1774	novel food grade nanoparticles	1745:1774	novel food grade nanoparticles with desirable stability and digestion behaviour	1745:1823	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	10	77	theme	sustained	1427:1435	arg1	release					1437:1443	the best sustained release	1418:1443	the best sustained release in the small intestine	1418:1466	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	0	78	theme	saponin	66:72	arg1	complex					74:80	resveratrol-loaded protein-polysaccharide-tea saponin complex	20:80	resveratrol-loaded protein-polysaccharide-tea saponin complex	20:80	The construction of resveratrol-loaded protein-polysaccharide-tea saponin complex nanoparticles for controlling physicochemical stability and in vitro digestion.					
33118591	3	79	theme	nanoparticles	494:506	arg1	size					466:469	The size	462:469	The size of zein-PGA-TS complex nanoparticles	462:506	The size of zein-PGA-TS complex nanoparticles was between 281.9 and 309.7 nm.					
33118591	4	80	theme	encapsulation	567:579	arg1	efficiency					581:590	the encapsulation efficiency	563:590	the encapsulation efficiency of resveratrol	563:605	In the presence of TS, the encapsulation efficiency of resveratrol was significantly elevated from 58.43% to 85.58%.					
33118591	11	81	theme	novel	1745:1749	arg1	nanoparticles					1762:1774	novel food grade nanoparticles	1745:1774	novel food grade nanoparticles with desirable stability and digestion behaviour	1745:1823	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	4	82	dep	TS	559:560	arg1	the					543:545	the	543:545	the	543:545	In the presence of TS, the encapsulation efficiency of resveratrol was significantly elevated from 58.43% to 85.58%.					
33118591	4	82	dep	TS	559:560	arg1	presence					547:554	presence	547:554	presence	547:554	In the presence of TS, the encapsulation efficiency of resveratrol was significantly elevated from 58.43% to 85.58%.					
33118591	10	83	theme	small	1452:1456	arg1	intestine					1458:1466	the small intestine	1448:1466	the small intestine	1448:1466	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	10	84	theme	mass	1477:1480	arg1	ratio					1482:1486	the mass ratio	1473:1486	the mass ratio of zein to TS	1473:1500	Moreover, the zein-PGA-TS complex nanoparticles exhibited the best sustained release in the small intestine when the mass ratio of zein to TS was 5 : 1 (23.20% in the stomach and 63.11% in the small intestine).					
33118591	6	85	theme	Multiple	795:802	arg1	methods					818:824	Multiple spectroscopic methods	795:824	Multiple spectroscopic methods	795:824	Multiple spectroscopic methods revealed that TS altered the micro-environment and secondary structure of the protein.					
33118591	8	86	theme	scanning	1048:1055	arg1	calorimetry					1057:1067	Differential scanning calorimetry	1035:1067	Differential scanning calorimetry (DSC)	1035:1073	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	8	86	theme	scanning	1048:1055	arg1	DSC					1070:1072	DSC	1070:1072	DSC	1070:1072	Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) patterns showed the amorphous nature of the encapsulated resveratrol.					
33118591	11	87	theme	new	1709:1711	arg1	insight					1713:1719	a new insight	1707:1719	a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour	1707:1823	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	11	88	theme	protein-polysaccharide	1658:1679	arg1	complexes					1681:1689	the protein-polysaccharide complexes	1654:1689	the protein-polysaccharide complexes	1654:1689	These findings indicated the influence of TS on the properties and applications of the protein-polysaccharide complexes, which provided a new insight into the development of novel food grade nanoparticles with desirable stability and digestion behaviour.					
33118591	2	89	theme	gastrointestinal	405:420	arg1	attributes					350:359	the functional attributes	335:359	the functional attributes	335:359	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33118591	2	89	theme	gastrointestinal	405:420	arg1	digestion					422:430	gastrointestinal digestion	405:430	gastrointestinal digestion of the complex nanoparticles	405:459	TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles.					
33964269	11	0	theme	1,4	1559:1561	arg1	linkage					1563:1569	the 1,4 linkage	1555:1569	the 1,4 linkage between α-d-GalN and β-d-GlcA	1555:1599	Thiopeptidoglycan lyase was thus confirmed to cleave the 1,4 linkage between α-d-GalN and β-d-GlcA, regardless of the peptide moiety.					
33964269	1	1	theme	Sphaerotilus	181:192	arg1	bacterium					233:241	a filamentous sheath-forming bacterium	204:241	a filamentous sheath-forming bacterium commonly found in activated sludge	204:276	Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge.					
33964269	1	1	theme	Sphaerotilus	181:192	arg1	natans					194:199	Sphaerotilus natans	181:199	Sphaerotilus natans	181:199	Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge.					
33964269	5	2	theme	cysteine	700:707	arg1	free					692:695	free	692:695	free	692:695	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	5	2	theme	cysteine	700:707	arg1	sheath					682:687	the S. montanus sheath	666:687	the S. montanus sheath	666:687	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	0	3	theme	thiopeptidoglycan	93:109	arg1	lyase					111:115	thiopeptidoglycan lyase	93:115	thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-d-GalN and β-d-GlcA	93:178	Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-d-GalN and β-d-GlcA.					
33964269	4	4	dep	S.	570:571	arg1	natans					573:578	natans	573:578	natans	573:578	However, it exhibits heat susceptibility, which distinguishes it from the S. natans sheath.					
33964269	5	5	theme	solid-state	628:638	arg1	NMR					640:642	solid-state NMR	628:642	solid-state NMR	628:642	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	10	6	theme	[→4	1315:1317	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	3	7	theme	stream	415:420	arg1	biofilms					422:429	stream biofilms	415:429	stream biofilms	415:429	S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans.					
33964269	6	8	theme	solution-state	862:875	arg1	analysis					881:888	solution-state NMR analysis	862:888	solution-state NMR analysis	862:888	The S. montanus sheath was successfully solubilized by N-acetylation, allowing solution-state NMR analysis to determine the sugar sequence.					
33964269	8	9	theme	4-aminobenzoic	1125:1138	arg1	ester					1151:1155	4-aminobenzoic acid ethyl ester	1125:1155	4-aminobenzoic acid ethyl ester	1125:1155	The reducing ends of the enzymatic digests were labeled with 4-aminobenzoic acid ethyl ester, followed by HPLC.					
33964269	5	10	theme	NMR	640:642	arg1	analyses					644:651	chemical composition and solid-state NMR analyses	603:651	chemical composition and solid-state NMR analyses	603:651	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	3	11	theme	biofilms	422:429	arg1	ATCC-BAA-2725					371:383	S. montanus ATCC-BAA-2725	359:383	S. montanus ATCC-BAA-2725	359:383	S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans.					
33964269	3	11	theme	biofilms	422:429	arg1	member					405:410	a sheath-forming member	388:410	a sheath-forming member of stream biofilms	388:429	S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans.					
33964269	4	12	theme	heat	517:520	arg1	susceptibility					522:535	heat susceptibility	517:535	heat susceptibility	517:535	However, it exhibits heat susceptibility, which distinguishes it from the S. natans sheath.					
33964269	11	13	theme	peptide	1620:1626	arg1	moiety					1628:1633	the peptide moiety	1616:1633	the peptide moiety	1616:1633	Thiopeptidoglycan lyase was thus confirmed to cleave the 1,4 linkage between α-d-GalN and β-d-GlcA, regardless of the peptide moiety.					
33964269	10	14	theme	1→	1393:1394	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	10	15	theme	-β-d-Glc-	1334:1342	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	0	16	theme	1,4	138:140	arg1	linkage					142:148	the 1,4 linkage	134:148	the 1,4 linkage between α-d-GalN and β-d-GlcA	134:178	Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-d-GalN and β-d-GlcA.					
33964269	12	17	theme	sheath	1683:1688	arg1	staining					1667:1674	vital fluorescent staining	1649:1674	vital fluorescent staining of the sheath	1649:1688	Furthermore, vital fluorescent staining of the sheath demonstrated that elongation takes place at the tips, as with the S. natans sheath.					
33964269	8	18	theme	ethyl	1145:1149	arg1	ester					1151:1155	4-aminobenzoic acid ethyl ester	1125:1155	4-aminobenzoic acid ethyl ester	1125:1155	The reducing ends of the enzymatic digests were labeled with 4-aminobenzoic acid ethyl ester, followed by HPLC.					
33964269	8	19	theme	reducing	1068:1075	arg1	ends					1077:1080	The reducing ends	1064:1080	The reducing ends of the enzymatic digests	1064:1105	The reducing ends of the enzymatic digests were labeled with 4-aminobenzoic acid ethyl ester, followed by HPLC.					
33964269	2	20	theme	thiolic	310:316	arg1	glycoconjugate					318:331	a thiolic glycoconjugate	308:331	a thiolic glycoconjugate called thiopeptidoglycan	308:356	Its sheath is assembled from a thiolic glycoconjugate called thiopeptidoglycan.					
33964269	3	21	theme	S.	485:486	arg1	natans					488:493	S. natans	485:493	S. natans	485:493	S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans.					
33964269	8	22	theme	acid	1140:1143	arg1	ester					1151:1155	4-aminobenzoic acid ethyl ester	1125:1155	4-aminobenzoic acid ethyl ester	1125:1155	The reducing ends of the enzymatic digests were labeled with 4-aminobenzoic acid ethyl ester, followed by HPLC.					
33964269	10	23	contain	has	1270:1272	arg2	peptides					1277:1284	no peptides	1274:1284	no peptides	1274:1284	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	10	23	contain	has	1270:1272	arg1	sheath					1263:1268	the sheath	1259:1268	the sheath	1259:1268	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	10	23	contain	has	1270:1272	arg1	follows					1306:1312	follows	1306:1312	follows	1306:1312	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	10	24	theme	1→4	1330:1332	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	5	25	theme	sheath	765:770	arg1	formation					772:780	sheath formation	765:780	sheath formation	765:780	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	5	26	theme	S.	670:671	arg1	free					692:695	free	692:695	free	692:695	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	5	26	theme	S.	670:671	arg1	sheath					682:687	the S. montanus sheath	666:687	the S. montanus sheath	666:687	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	5	27	theme	chemical	603:610	arg1	composition					612:622	chemical composition	603:622	chemical composition	603:622	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	1	28	theme	filamentous	206:216	arg1	bacterium					233:241	a filamentous sheath-forming bacterium	204:241	a filamentous sheath-forming bacterium commonly found in activated sludge	204:276	Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge.					
33964269	1	28	theme	filamentous	206:216	arg1	natans					194:199	Sphaerotilus natans	181:199	Sphaerotilus natans	181:199	Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge.					
33964269	0	29	theme	Structural	0:9	arg1	determination					11:23	Structural determination	0:23	Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus	0:85	Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-d-GalN and β-d-GlcA.					
33964269	5	30	theme	montanus	673:680	arg1	free					692:695	free	692:695	free	692:695	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	5	30	theme	montanus	673:680	arg1	sheath					682:687	the S. montanus sheath	666:687	the S. montanus sheath	666:687	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	5	31	theme	composition	612:622	arg1	analyses					644:651	chemical composition and solid-state NMR analyses	603:651	chemical composition and solid-state NMR analyses	603:651	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	10	32	theme	-β-d-GalNAc-	1348:1359	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	1	33	theme	sheath-forming	218:231	arg1	bacterium					233:241	a filamentous sheath-forming bacterium	204:241	a filamentous sheath-forming bacterium commonly found in activated sludge	204:276	Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge.					
33964269	1	33	theme	sheath-forming	218:231	arg1	natans					194:199	Sphaerotilus natans	181:199	Sphaerotilus natans	181:199	Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge.					
33964269	8	34	theme	digests	1099:1105	arg1	ends					1077:1080	The reducing ends	1064:1080	The reducing ends of the enzymatic digests	1064:1105	The reducing ends of the enzymatic digests were labeled with 4-aminobenzoic acid ethyl ester, followed by HPLC.					
33964269	5	35	theme	disulfide	726:734	arg1	linkage					736:742	disulfide linkage	726:742	disulfide linkage	726:742	In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation.					
33964269	8	36	theme	enzymatic	1089:1097	arg1	digests					1099:1105	the enzymatic digests	1085:1105	the enzymatic digests	1085:1105	The reducing ends of the enzymatic digests were labeled with 4-aminobenzoic acid ethyl ester, followed by HPLC.					
33964269	7	37	theme	Paenibacillus	1037:1049	arg1	bacterium					1026:1034	the thiopeptidoglycan-assimilating bacterium	991:1034	the thiopeptidoglycan-assimilating bacterium	991:1034	The sheath was susceptible to thiopeptidoglycan lyase prepared from the thiopeptidoglycan-assimilating bacterium, Paenibacillus koleovorans.					
33964269	7	37	theme	Paenibacillus	1037:1049	arg1	koleovorans					1051:1061	Paenibacillus koleovorans	1037:1061	Paenibacillus koleovorans	1037:1061	The sheath was susceptible to thiopeptidoglycan lyase prepared from the thiopeptidoglycan-assimilating bacterium, Paenibacillus koleovorans.					
33964269	10	38	theme	1→3	1344:1346	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	12	39	theme	fluorescent	1655:1665	arg1	staining					1667:1674	vital fluorescent staining	1649:1674	vital fluorescent staining of the sheath	1649:1688	Furthermore, vital fluorescent staining of the sheath demonstrated that elongation takes place at the tips, as with the S. natans sheath.					
33964269	0	40	theme	polysaccharide	47:60	arg1	determination					11:23	Structural determination	0:23	Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus	0:85	Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-d-GalN and β-d-GlcA.					
33964269	6	41	theme	sugar	907:911	arg1	sequence					913:920	the sugar sequence	903:920	the sugar sequence	903:920	The S. montanus sheath was successfully solubilized by N-acetylation, allowing solution-state NMR analysis to determine the sugar sequence.					
33964269	0	42	theme	sheath-forming	32:45	arg1	polysaccharide					47:60	the sheath-forming polysaccharide	28:60	the sheath-forming polysaccharide of Sphaerotilus montanus	28:85	Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-d-GalN and β-d-GlcA.					
33964269	4	43	theme	S.	570:571	arg1	sheath					580:585	the S. natans sheath	566:585	the S. natans sheath	566:585	However, it exhibits heat susceptibility, which distinguishes it from the S. natans sheath.					
33964269	6	44	theme	montanus	790:797	arg1	sheath					799:804	The S. montanus sheath	783:804	The S. montanus sheath	783:804	The S. montanus sheath was successfully solubilized by N-acetylation, allowing solution-state NMR analysis to determine the sugar sequence.					
33964269	0	45	theme	Sphaerotilus	65:76	arg1	montanus					78:85	Sphaerotilus montanus	65:85	Sphaerotilus montanus	65:85	Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-d-GalN and β-d-GlcA.					
33964269	3	46	theme	S.	359:360	arg1	ATCC-BAA-2725					371:383	S. montanus ATCC-BAA-2725	359:383	S. montanus ATCC-BAA-2725	359:383	S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans.					
33964269	3	46	theme	S.	359:360	arg1	member					405:410	a sheath-forming member	388:410	a sheath-forming member of stream biofilms	388:429	S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans.					
33964269	3	47	theme	sheath-forming	390:403	arg1	ATCC-BAA-2725					371:383	S. montanus ATCC-BAA-2725	359:383	S. montanus ATCC-BAA-2725	359:383	S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans.					
33964269	3	47	theme	sheath-forming	390:403	arg1	member					405:410	a sheath-forming member	388:410	a sheath-forming member of stream biofilms	388:429	S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans.					
33964269	6	48	theme	S.	787:788	arg1	sheath					799:804	The S. montanus sheath	783:804	The S. montanus sheath	783:804	The S. montanus sheath was successfully solubilized by N-acetylation, allowing solution-state NMR analysis to determine the sugar sequence.					
33964269	3	49	theme	montanus	362:369	arg1	ATCC-BAA-2725					371:383	S. montanus ATCC-BAA-2725	359:383	S. montanus ATCC-BAA-2725	359:383	S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans.					
33964269	3	49	theme	montanus	362:369	arg1	member					405:410	a sheath-forming member	388:410	a sheath-forming member of stream biofilms	388:429	S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans.					
33964269	10	50	dep	follows	1306:1312	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	10	51	theme	1→4	1361:1363	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	7	52	theme	thiopeptidoglycan	953:969	arg1	lyase					971:975	thiopeptidoglycan lyase	953:975	thiopeptidoglycan lyase prepared from the thiopeptidoglycan-assimilating bacterium, Paenibacillus koleovorans	953:1061	The sheath was susceptible to thiopeptidoglycan lyase prepared from the thiopeptidoglycan-assimilating bacterium, Paenibacillus koleovorans.					
33964269	12	53	theme	S.	1756:1757	arg1	sheath					1766:1771	the S. natans sheath	1752:1771	the S. natans sheath	1752:1771	Furthermore, vital fluorescent staining of the sheath demonstrated that elongation takes place at the tips, as with the S. natans sheath.					
33964269	12	54	theme	vital	1649:1653	arg1	staining					1667:1674	vital fluorescent staining	1649:1674	vital fluorescent staining of the sheath	1649:1688	Furthermore, vital fluorescent staining of the sheath demonstrated that elongation takes place at the tips, as with the S. natans sheath.					
33964269	1	55	theme	activated	261:269	arg1	sludge					271:276	activated sludge	261:276	activated sludge	261:276	Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge.					
33964269	6	56	theme	NMR	877:879	arg1	analysis					881:888	solution-state NMR analysis	862:888	solution-state NMR analysis	862:888	The S. montanus sheath was successfully solubilized by N-acetylation, allowing solution-state NMR analysis to determine the sugar sequence.					
33964269	10	57	theme	-α-d-GalNAc-	1365:1376	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	10	58	theme	-α-d-GalN-	1382:1391	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	7	59	theme	thiopeptidoglycan-assimilating	995:1024	arg1	bacterium					1026:1034	the thiopeptidoglycan-assimilating bacterium	991:1034	the thiopeptidoglycan-assimilating bacterium	991:1034	The sheath was susceptible to thiopeptidoglycan lyase prepared from the thiopeptidoglycan-assimilating bacterium, Paenibacillus koleovorans.					
33964269	7	59	theme	thiopeptidoglycan-assimilating	995:1024	arg1	koleovorans					1051:1061	Paenibacillus koleovorans	1037:1061	Paenibacillus koleovorans	1037:1061	The sheath was susceptible to thiopeptidoglycan lyase prepared from the thiopeptidoglycan-assimilating bacterium, Paenibacillus koleovorans.					
33964269	12	60	theme	natans	1759:1764	arg1	sheath					1766:1771	the S. natans sheath	1752:1771	the S. natans sheath	1752:1771	Furthermore, vital fluorescent staining of the sheath demonstrated that elongation takes place at the tips, as with the S. natans sheath.					
33964269	1	61	located	found	252:256	arg2	natans					194:199	Sphaerotilus natans	181:199	Sphaerotilus natans	181:199	Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge.					
33964269	1	61	located	found	252:256	arg1	sludge					271:276	activated sludge	261:276	activated sludge	261:276	Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge.					
33964269	1	61	located	found	252:256	arg2	bacterium					233:241	a filamentous sheath-forming bacterium	204:241	a filamentous sheath-forming bacterium commonly found in activated sludge	204:276	Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge.					
33964269	11	62	theme	Thiopeptidoglycan	1502:1518	arg1	lyase					1520:1524	Thiopeptidoglycan lyase	1502:1524	Thiopeptidoglycan lyase	1502:1524	Thiopeptidoglycan lyase was thus confirmed to cleave the 1,4 linkage between α-d-GalN and β-d-GlcA, regardless of the peptide moiety.					
33964269	0	63	theme	montanus	78:85	arg1	polysaccharide					47:60	the sheath-forming polysaccharide	28:60	the sheath-forming polysaccharide of Sphaerotilus montanus	28:85	Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-d-GalN and β-d-GlcA.					
33964269	10	64	theme	1→4	1378:1380	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
33964269	10	65	theme	-β-d-GlcA-	1319:1328	arg1	n					1396:1396	[→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1315:1396	follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n	1306:1396	We found that the sheath has no peptides and is assembled as follows: [→4)-β-d-GlcA-(1→4)-β-d-Glc-(1→3)-β-d-GalNAc-(1→4)-α-d-GalNAc-(1→4)-α-d-GalN-(1→]n (β-d-Glc and α-d-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively).					
32441772	1	0	dep	wall	273:276	arg1	composition					278:288	composition	278:288	composition	278:288	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	6	1	theme	syringyl	1135:1142	arg1	units					1144:1148	syringyl units	1135:1148	syringyl units	1135:1148	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots.					
32441772	1	2	theme	abiotic	225:231	arg1	stress					233:238	abiotic stress	225:238	abiotic stress	225:238	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	7	3	theme	enzymes	1245:1251	arg1	activity					1233:1240	the activity	1229:1240	the activity of enzymes enrolled in phenylpropanoid biosynthesis	1229:1292	Salt stress also induced the expression of genes and the activity of enzymes enrolled in phenylpropanoid biosynthesis.					
32441772	7	3	theme	enzymes	1245:1251	arg1	expression					1205:1214	the expression	1201:1214	the expression of genes	1201:1223	Salt stress also induced the expression of genes and the activity of enzymes enrolled in phenylpropanoid biosynthesis.					
32441772	9	4	attach	present	1494:1500	arg2	we					1491:1492	we	1491:1492	we	1491:1492	In conclusion, we present a model for explaining cell wall remodeling in response to salinity.					
32441772	9	4	attach	present	1494:1500	arg1	conclusion					1479:1488	conclusion	1479:1488	conclusion	1479:1488	In conclusion, we present a model for explaining cell wall remodeling in response to salinity.					
32441772	4	5	theme	gel	777:779	arg1	electrophoresis					781:795	carbohydrate gel electrophoresis	764:795	carbohydrate gel electrophoresis	764:795	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	2	6	from	wall	399:402	arg1	study					374:378	an in-depth study	362:378	an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants	362:490	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	4	7	dep	extraction	665:674	arg1	The					661:663	The	661:663	The	661:663	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	8	8	theme	derivatives	1435:1445	arg1	modulation					1349:1358	the modulation	1345:1358	the modulation of phenolic profiling by salinity	1345:1392	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	8	8	theme	derivatives	1435:1445	arg1	accumulation					1402:1413	the accumulation	1398:1413	the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate	1398:1473	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	1	9	from	wall	273:276	arg1	grasses					308:314	grasses	308:314	grasses	308:314	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	6	10	from	incorporation	1118:1130	arg1	lignin					1153:1158	lignin	1153:1158	lignin of maize roots	1153:1173	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots.					
32441772	4	11	theme	2D-NMR	740:745	arg1	spectroscopy					747:758	2D-NMR spectroscopy	740:758	2D-NMR spectroscopy	740:758	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	8	12	theme	metabolite	1314:1323	arg1	profiling					1325:1333	The UHPLC-MS-based metabolite profiling	1295:1333	The UHPLC-MS-based metabolite profiling	1295:1333	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	7	13	theme	genes	1219:1223	arg1	activity					1233:1240	the activity	1229:1240	the activity of enzymes enrolled in phenylpropanoid biosynthesis	1229:1292	Salt stress also induced the expression of genes and the activity of enzymes enrolled in phenylpropanoid biosynthesis.					
32441772	7	13	theme	genes	1219:1223	arg1	expression					1205:1214	the expression	1201:1214	the expression of genes	1201:1223	Salt stress also induced the expression of genes and the activity of enzymes enrolled in phenylpropanoid biosynthesis.					
32441772	4	14	theme	salt-stressed	843:855	arg1	roots					857:861	salt-stressed roots	843:861	salt-stressed roots	843:861	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	8	15	theme	UHPLC-MS-based	1299:1312	arg1	profiling					1325:1333	The UHPLC-MS-based metabolite profiling	1295:1333	The UHPLC-MS-based metabolite profiling	1295:1333	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	3	16	theme	Cell	493:496	arg1	characterization					503:518	Cell wall characterization	493:518	Cell wall characterization	493:518	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	1	17	theme	cell	155:158	arg1	polymers					165:172	cell wall polymers	155:172	cell wall polymers	155:172	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	6	18	theme	lignin	1026:1031	arg1	content					1033:1039	lignin content	1026:1039	lignin content	1026:1039	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots.					
32441772	3	19	theme	wall	498:501	arg1	characterization					503:518	Cell wall characterization	493:518	Cell wall characterization	493:518	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	1	20	theme	wall	160:163	arg1	polymers					165:172	cell wall polymers	155:172	cell wall polymers	155:172	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	0	21	theme	wall	5:8	arg1	remodeling					10:19	Cell wall remodeling	0:19	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.	0:144	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.					
32441772	2	22	from	metabolism	429:438	arg1	study					374:378	an in-depth study	362:378	an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants	362:490	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	1	23	from	effects	245:251	arg1	wall					273:276	cell wall composition and metabolism in grasses	268:314	wall	273:276	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	1	23	from	effects	245:251	arg1	metabolism					294:303	metabolism	294:303	metabolism in grasses	294:314	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	5	24	from	feruloylation	944:956	arg1	roots					978:982	roots	978:982	roots of seedlings and plants	978:1006	Saponification and mild acid hydrolysis revealed that salinity also reduced the feruloylation of arabinoxylans in roots of seedlings and plants.					
32441772	4	25	theme	size-exclusion	709:722	arg1	chromatography					724:737	size-exclusion chromatography	709:737	size-exclusion chromatography	709:737	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	5	26	theme	mild	883:886	arg1	hydrolysis					893:902	mild acid hydrolysis	883:902	mild acid hydrolysis	883:902	Saponification and mild acid hydrolysis revealed that salinity also reduced the feruloylation of arabinoxylans in roots of seedlings and plants.					
32441772	0	27	theme	Cell	0:3	arg1	remodeling					10:19	Cell wall remodeling	0:19	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.	0:144	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.					
32441772	2	28	dep	wall	399:402	arg1	composition					404:414	composition	404:414	composition	404:414	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	5	29	theme	arabinoxylans	961:973	arg1	feruloylation					944:956	the feruloylation	940:956	the feruloylation of arabinoxylans in roots of seedlings and plants	940:1006	Saponification and mild acid hydrolysis revealed that salinity also reduced the feruloylation of arabinoxylans in roots of seedlings and plants.					
32441772	8	30	theme	ferulate	1418:1425	arg1	modulation					1349:1358	the modulation	1345:1358	the modulation of phenolic profiling by salinity	1345:1392	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	8	30	theme	ferulate	1418:1425	arg1	accumulation					1402:1413	the accumulation	1398:1413	the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate	1398:1473	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	5	31	theme	acid	888:891	arg1	hydrolysis					893:902	mild acid hydrolysis	883:902	mild acid hydrolysis	883:902	Saponification and mild acid hydrolysis revealed that salinity also reduced the feruloylation of arabinoxylans in roots of seedlings and plants.					
32441772	2	32	theme	cell	394:397	arg1	wall					399:402	cell wall composition and phenolic metabolism	394:438	wall	399:402	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	8	33	theme	phenolic	1363:1370	arg1	profiling					1372:1380	phenolic profiling	1363:1380	phenolic profiling	1363:1380	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	3	34	from	deposition	560:569	arg1	stems					654:658	stems	654:658	stems	654:658	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	3	34	from	deposition	560:569	arg1	roots					631:635	seedling roots	622:635	seedling roots	622:635	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	3	34	from	deposition	560:569	arg1	roots					644:648	plant roots	638:648	plant roots	638:648	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	2	35	theme	changes	383:389	arg1	study					374:378	an in-depth study	362:378	an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants	362:490	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	1	36	theme	salinity	256:263	arg1	effects					245:251	the effects	241:251	the effects of salinity on cell wall composition and metabolism in grasses	241:314	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	4	37	theme	carbohydrate	764:775	arg1	electrophoresis					781:795	carbohydrate gel electrophoresis	764:795	carbohydrate gel electrophoresis	764:795	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	3	38	theme	seedling	622:629	arg1	roots					631:635	seedling roots	622:635	seedling roots	622:635	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	3	39	theme	salt	534:537	arg1	stress					539:544	salt stress	534:544	salt stress	534:544	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	5	40	theme	seedlings	987:995	arg1	roots					978:982	roots	978:982	roots of seedlings and plants	978:1006	Saponification and mild acid hydrolysis revealed that salinity also reduced the feruloylation of arabinoxylans in roots of seedlings and plants.					
32441772	6	41	theme	maize	1163:1167	arg1	roots					1169:1173	maize roots	1163:1173	maize roots	1163:1173	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots.					
32441772	8	42	theme	4-O-feruloyl	1454:1465	arg1	quinate					1467:1473	4-O-feruloyl quinate	1454:1473	4-O-feruloyl quinate	1454:1473	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	9	43	theme	wall	1530:1533	arg1	remodeling					1535:1544	cell wall remodeling	1525:1544	cell wall remodeling in response to salinity	1525:1568	In conclusion, we present a model for explaining cell wall remodeling in response to salinity.					
32441772	0	44	theme	salt	27:30	arg1	stress					32:37	salt stress	27:37	salt stress	27:37	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.					
32441772	1	45	theme	cell	268:271	arg1	wall					273:276	cell wall composition and metabolism in grasses	268:314	wall	273:276	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	0	46	theme	phenolic	116:123	arg1	metabolism					125:134	phenolic metabolism	116:134	phenolic metabolism	116:134	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.					
32441772	0	47	from	changes	54:60	arg1	maize					139:143	maize	139:143	maize	139:143	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.					
32441772	0	47	from	changes	54:60	arg1	metabolism					125:134	phenolic metabolism	116:134	phenolic metabolism	116:134	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.					
32441772	0	47	from	changes	54:60	arg1	polysaccharides					65:79	polysaccharides	65:79	polysaccharides	65:79	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.					
32441772	0	47	from	changes	54:60	arg1	feruloylation					82:94	feruloylation	82:94	feruloylation	82:94	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.					
32441772	0	47	from	changes	54:60	arg1	lignification					97:109	lignification	97:109	lignification	97:109	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.					
32441772	3	48	theme	cellulose	574:582	arg1	deposition					560:569	the deposition	556:569	the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems	556:658	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	2	49	theme	in-depth	365:372	arg1	study					374:378	an in-depth study	362:378	an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants	362:490	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	1	50	from	metabolism	294:303	arg1	grasses					308:314	grasses	308:314	grasses	308:314	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	6	51	theme	nitrobenzene	1060:1071	arg1	oxidation					1073:1081	nitrobenzene oxidation	1060:1081	nitrobenzene oxidation	1060:1081	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots.					
32441772	4	52	theme	content	832:838	arg1	reduction					806:814	a reduction	804:814	a reduction of arabinoxylan content in salt-stressed roots	804:861	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	9	53	from	remodeling	1535:1544	arg1	response					1549:1556	response	1549:1556	response to salinity	1549:1568	In conclusion, we present a model for explaining cell wall remodeling in response to salinity.					
32441772	6	54	theme	roots	1169:1173	arg1	lignin					1153:1158	lignin	1153:1158	lignin of maize roots	1153:1173	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots.					
32441772	7	55	theme	Salt	1176:1179	arg1	stress					1181:1186	Salt stress	1176:1186	Salt stress	1176:1186	Salt stress also induced the expression of genes and the activity of enzymes enrolled in phenylpropanoid biosynthesis.					
32441772	4	56	theme	arabinoxylan	819:830	arg1	content					832:838	arabinoxylan content	819:838	arabinoxylan content	819:838	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	9	57	theme	cell	1525:1528	arg1	remodeling					1535:1544	cell wall remodeling	1525:1544	cell wall remodeling in response to salinity	1525:1568	In conclusion, we present a model for explaining cell wall remodeling in response to salinity.					
32441772	3	58	theme	lignin	612:617	arg1	deposition					560:569	the deposition	556:569	the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems	556:658	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	2	59	dep	maize	465:469	arg1	seedlings					471:479	seedlings	471:479	seedlings	471:479	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	8	60	dep	derivatives	1435:1445	arg1	3-					1447:1448	3-	1447:1448	3-	1447:1448	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	3	61	theme	polysaccharides	592:606	arg1	deposition					560:569	the deposition	556:569	the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems	556:658	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	4	62	from	reduction	806:814	arg1	roots					857:861	salt-stressed roots	843:861	salt-stressed roots	843:861	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	4	63	theme	arabinoxylans	692:704	arg1	analysis					680:687	analysis	680:687	analysis	680:687	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	4	63	theme	arabinoxylans	692:704	arg1	extraction					665:674	extraction	665:674	extraction	665:674	The extraction and analysis of arabinoxylans by size-exclusion chromatography, 2D-NMR spectroscopy and carbohydrate gel electrophoresis showed a reduction of arabinoxylan content in salt-stressed roots.					
32441772	5	64	theme	plants	1001:1006	arg1	roots					978:982	roots	978:982	roots of seedlings and plants	978:1006	Saponification and mild acid hydrolysis revealed that salinity also reduced the feruloylation of arabinoxylans in roots of seedlings and plants.					
32441772	0	65	dep	remodeling	10:19	arg1	Insights					40:47	Insights	40:47	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.	0:144	Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize.					
32441772	2	66	from	study	374:378	arg1	wall					399:402	cell wall composition and phenolic metabolism	394:438	wall	399:402	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	2	66	from	study	374:378	arg1	metabolism					429:438	phenolic metabolism	420:438	phenolic metabolism	420:438	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	8	67	theme	profiling	1372:1380	arg1	modulation					1349:1358	the modulation	1345:1358	the modulation of phenolic profiling by salinity	1345:1392	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	8	67	theme	profiling	1372:1380	arg1	accumulation					1402:1413	the accumulation	1398:1413	the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate	1398:1473	The UHPLC-MS-based metabolite profiling confirmed the modulation of phenolic profiling by salinity and the accumulation of ferulate and its derivatives 3- and 4-O-feruloyl quinate.					
32441772	6	68	theme	composition	1045:1055	arg1	Determination					1009:1021	Determination	1009:1021	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR	1009:1092	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots.					
32441772	3	69	theme	plant	638:642	arg1	roots					644:648	plant roots	638:648	plant roots	638:648	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	1	70	theme	important	179:187	arg1	roles					189:193	important roles	179:193	important roles	179:193	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	6	71	theme	increased	1108:1116	arg1	incorporation					1118:1130	the increased incorporation	1104:1130	the increased incorporation of syringyl units in lignin of maize roots	1104:1173	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots.					
32441772	1	72	theme	plants	215:220	arg1	tolerance					202:210	the tolerance	198:210	the tolerance of plants to abiotic stress	198:238	Although cell wall polymers play important roles in the tolerance of plants to abiotic stress, the effects of salinity on cell wall composition and metabolism in grasses remain largely unexplored.					
32441772	2	73	theme	phenolic	420:427	arg1	metabolism					429:438	phenolic metabolism	420:438	phenolic metabolism	420:438	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	3	74	theme	matrix	585:590	arg1	polysaccharides					592:606	matrix polysaccharides	585:606	matrix polysaccharides	585:606	Cell wall characterization revealed that salt stress modulated the deposition of cellulose, matrix polysaccharides and lignin in seedling roots, plant roots and stems.					
32441772	2	75	from	changes	383:389	arg1	wall					399:402	cell wall composition and phenolic metabolism	394:438	wall	399:402	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	2	75	from	changes	383:389	arg1	metabolism					429:438	phenolic metabolism	420:438	phenolic metabolism	420:438	Here, we conducted an in-depth study of changes in cell wall composition and phenolic metabolism induced upon salinity in maize seedlings and plants.					
32441772	6	76	theme	units	1144:1148	arg1	incorporation					1118:1130	the increased incorporation	1104:1130	the increased incorporation of syringyl units in lignin of maize roots	1104:1173	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots.					
32441772	7	77	theme	phenylpropanoid	1265:1279	arg1	biosynthesis					1281:1292	phenylpropanoid biosynthesis	1265:1292	phenylpropanoid biosynthesis	1265:1292	Salt stress also induced the expression of genes and the activity of enzymes enrolled in phenylpropanoid biosynthesis.					
32441772	6	78	theme	content	1033:1039	arg1	Determination					1009:1021	Determination	1009:1021	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR	1009:1092	Determination of lignin content and composition by nitrobenzene oxidation and 2D-NMR confirmed the increased incorporation of syringyl units in lignin of maize roots.					
32028238	5	0	dep	contents	763:770	arg1	measured					794:801	measured	794:801	were measured every two weeks	789:817	TC, TG contents in blood samples were measured every two weeks, and HDL-C, LDL-C was measured at 12 and 14 weeks evaluating of blood lipids regulation activity of polysaccharide.					
32028238	11	1	theme	community	2012:2020	arg1	diversity					1974:1982	diversity	1974:1982	diversity	1974:1982	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	11	1	theme	community	2012:2020	arg1	abundance					1960:1968	abundance	1960:1968	abundance	1960:1968	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	10	2	theme	rats	1671:1674	arg1	composition					1627:1637	the gut microbiota composition	1608:1637	the gut microbiota composition	1608:1637	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	10	2	theme	rats	1671:1674	arg1	diversity					1654:1662	diversity	1654:1662	diversity	1654:1662	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	10	2	theme	rats	1671:1674	arg1	abundance					1640:1648	abundance	1640:1648	abundance	1640:1648	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	12	3	theme	barrier	2146:2152	arg1	recovery					2106:2113	recovery	2106:2113	recovery of the intestinal permeability barrier	2106:2152	This promotes recovery of the intestinal permeability barrier, inhibits LPS entry into the circulation, alleviates inflammation, and prevents glucose and lipid metabolic disorders.					
32028238	10	4	theme	intestinal	1801:1810	arg1	permeability					1812:1823	intestinal permeability	1801:1823	intestinal permeability	1801:1823	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	3	5	theme	uronic	544:549	arg1	content					556:562	uronic acid content	544:562	uronic acid content	544:562	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	9	6	theme	diabetic	1542:1549	arg1	symptoms					1551:1558	diabetic symptoms	1542:1558	diabetic symptoms	1542:1558	RESULTS We found that PS and PSF improved both diabetic symptoms and lipid metabolism.					
32028238	4	7	theme	molecular	648:656	arg1	PSF					666:668	PSF	666:668	PSF	666:668	Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period.					
32028238	4	7	theme	molecular	648:656	arg1	weight					658:663	high molecular weight	643:663	high molecular weight (PSF) polysaccharides (> 100 KDa)	643:697	Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period.					
32028238	10	8	theme	gut	1612:1614	arg1	composition					1627:1637	the gut microbiota composition	1608:1637	the gut microbiota composition	1608:1637	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	11	9	theme	relative	2045:2052	arg1	abundance					2054:2062	the relative abundance	2041:2062	the relative abundance of SCFA-producing bacteria	2041:2089	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	10	10	theme	gastrointestinal	1835:1850	arg1	inflammation					1852:1863	gastrointestinal inflammation	1835:1863	gastrointestinal inflammation	1835:1863	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	3	11	from	weight	480:485	arg1	kingianum					603:611	Polygonatum kingianum	591:611	Polygonatum kingianum	591:611	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	12	12	theme	lipid	2246:2250	arg1	disorders					2262:2270	glucose and lipid metabolic disorders	2234:2270	glucose and lipid metabolic disorders	2234:2270	This promotes recovery of the intestinal permeability barrier, inhibits LPS entry into the circulation, alleviates inflammation, and prevents glucose and lipid metabolic disorders.					
32028238	2	13	theme	high-fat	317:324	arg1	diet					326:329	high-fat diet	317:329	high-fat diet (HFD) rats	317:340	Polysaccharides from Polygonatum kingianum could regulate glucose and lipid metabolism in high-fat diet (HFD) rats.					
32028238	2	13	theme	high-fat	317:324	arg1	HFD					332:334	HFD	332:334	HFD	332:334	Polysaccharides from Polygonatum kingianum could regulate glucose and lipid metabolism in high-fat diet (HFD) rats.					
32028238	8	14	theme	protein	1323:1329	arg1	ZO-1					1331:1334	tight junction protein ZO-1	1308:1334	tight junction protein ZO-1	1308:1334	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	7	15	theme	intestinal	1264:1273	arg1	tract					1275:1279	the intestinal tract	1260:1279	the intestinal tract of rats	1260:1287	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	11	16	theme	intestinal	1991:2000	arg1	community					2012:2020	the intestinal microbial community	1987:2020	the intestinal microbial community	1987:2020	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	5	17	dep	lipids	889:894	arg1	regulation					896:905	regulation	896:905	regulation	896:905	TC, TG contents in blood samples were measured every two weeks, and HDL-C, LDL-C was measured at 12 and 14 weeks evaluating of blood lipids regulation activity of polysaccharide.					
32028238	10	18	theme	HFD	1667:1669	arg1	rats					1671:1674	HFD rats	1667:1674	HFD rats	1667:1674	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	1	19	theme	OBJECTIVE	109:117	arg1	medicine					166:173	a traditional Chinese medicine	144:173	a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia	144:224	OBJECTIVE Polygonatum kingianum is a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia.					
32028238	1	19	theme	OBJECTIVE	109:117	arg1	kingianum					131:139	OBJECTIVE Polygonatum kingianum	109:139	OBJECTIVE Polygonatum kingianum	109:139	OBJECTIVE Polygonatum kingianum is a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia.					
32028238	3	20	from	content	556:562	arg1	kingianum					603:611	Polygonatum kingianum	591:611	Polygonatum kingianum	591:611	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	11	21	dep	CONCLUSION	1897:1906	arg1	kingianum					1920:1928	kingianum	1920:1928	kingianum	1920:1928	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	8	22	theme	tissue	1363:1368	arg1	detection					1375:1383	detection	1375:1383	detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway	1375:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	8	22	theme	tissue	1363:1368	arg1	expression					1294:1303	The expression	1290:1303	The expression of tight junction protein ZO-1 and occludin of intestinal tissue	1290:1368	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	3	23	from	polysaccharide	525:538	arg1	kingianum					603:611	Polygonatum kingianum	591:611	Polygonatum kingianum	591:611	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	6	24	used	used	958:961	arg2	samples					945:951	The stool samples	935:951	The stool samples	935:951	The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora.					
32028238	1	25	theme	Chinese	158:164	arg1	medicine					166:173	a traditional Chinese medicine	144:173	a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia	144:224	OBJECTIVE Polygonatum kingianum is a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia.					
32028238	1	25	theme	Chinese	158:164	arg1	kingianum					131:139	OBJECTIVE Polygonatum kingianum	109:139	OBJECTIVE Polygonatum kingianum	109:139	OBJECTIVE Polygonatum kingianum is a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia.					
32028238	3	26	theme	permeation	359:368	arg1	METHODS					343:349	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	26	theme	permeation	359:368	arg1	GPC					386:388	GPC	386:388	GPC	386:388	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	26	theme	permeation	359:368	arg1	chromatography					370:383	The gel permeation chromatography	351:383	The gel permeation chromatography (GPC)	351:389	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	7	27	theme	acid	1123:1126	arg1	content					1094:1100	The content	1090:1100	The content of short chain fatty acid (SCFAs) in faeces	1090:1144	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	11	28	theme	CONCLUSION	1897:1906	arg1	polysaccharides					1930:1944	CONCLUSION Polygonatum kingianum polysaccharides	1897:1944	CONCLUSION Polygonatum kingianum polysaccharides	1897:1944	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	5	29	theme	blood	883:887	arg1	lipids					889:894	blood lipids	883:894	blood lipids regulation activity of polysaccharide	883:932	TC, TG contents in blood samples were measured every two weeks, and HDL-C, LDL-C was measured at 12 and 14 weeks evaluating of blood lipids regulation activity of polysaccharide.					
32028238	8	30	theme	key	1392:1394	arg1	expression					1404:1413	the key protein expression	1388:1413	the key protein expression in the LPS-TLR4/NFκB signaling pathway	1388:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	3	31	theme	chemical	425:432	arg1	analysis					434:441	chemical analysis	425:441	chemical analysis	425:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	31	theme	chemical	425:432	arg1	METHODS					343:349	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	7	32	theme	chain	1111:1115	arg1	SCFAs					1129:1133	SCFAs	1129:1133	SCFAs	1129:1133	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	7	32	theme	chain	1111:1115	arg1	acid					1123:1126	short chain fatty acid	1105:1126	short chain fatty acid (SCFAs)	1105:1134	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	6	33	theme	variable	986:993	arg1	method					1014:1019	rDNA V4 highly variable region measurement method	971:1019	rDNA V4 highly variable region measurement method	971:1019	The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora.					
32028238	8	34	theme	expression	1404:1413	arg1	detection					1375:1383	detection	1375:1383	detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway	1375:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	8	34	theme	expression	1404:1413	arg1	expression					1294:1303	The expression	1290:1303	The expression of tight junction protein ZO-1 and occludin of intestinal tissue	1290:1368	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	3	35	used	used	448:451	arg2	chromatography					370:383	The gel permeation chromatography	351:383	The gel permeation chromatography (GPC)	351:389	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	35	used	used	448:451	arg2	analysis					434:441	chemical analysis	425:441	chemical analysis	425:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	35	used	used	448:451	arg2	chromatography					399:412	liquid chromatography	392:412	liquid chromatography (HPLC)	392:419	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	35	used	used	448:451	arg2	METHODS					343:349	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	5	36	theme	TG	760:761	arg1	contents					763:770	TG contents	760:770	TG contents in blood samples were measured every two weeks	760:817	TC, TG contents in blood samples were measured every two weeks, and HDL-C, LDL-C was measured at 12 and 14 weeks evaluating of blood lipids regulation activity of polysaccharide.					
32028238	12	37	theme	metabolic	2252:2260	arg1	disorders					2262:2270	glucose and lipid metabolic disorders	2234:2270	glucose and lipid metabolic disorders	2234:2270	This promotes recovery of the intestinal permeability barrier, inhibits LPS entry into the circulation, alleviates inflammation, and prevents glucose and lipid metabolic disorders.					
32028238	6	38	theme	V4	976:977	arg1	method					1014:1019	rDNA V4 highly variable region measurement method	971:1019	rDNA V4 highly variable region measurement method	971:1019	The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora.					
32028238	11	39	theme	bacteria	2082:2089	arg1	abundance					2054:2062	the relative abundance	2041:2062	the relative abundance of SCFA-producing bacteria	2041:2089	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	10	40	theme	microbiota	1616:1625	arg1	composition					1627:1637	the gut microbiota composition	1608:1637	the gut microbiota composition	1608:1637	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	2	41	from	glucose	285:291	arg1	rats					337:340	high-fat diet (HFD) rats	317:340	high-fat diet (HFD) rats	317:340	Polysaccharides from Polygonatum kingianum could regulate glucose and lipid metabolism in high-fat diet (HFD) rats.					
32028238	8	42	theme	signaling	1436:1444	arg1	pathway					1446:1452	the LPS-TLR4/NFκB signaling pathway	1418:1452	the LPS-TLR4/NFκB signaling pathway	1418:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	10	43	theme	fatty	1725:1729	arg1	SCFA					1737:1740	SCFA	1737:1740	SCFA	1737:1740	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	10	43	theme	fatty	1725:1729	arg1	acid					1731:1734	short chain fatty acid	1713:1734	short chain fatty acid (SCFA)	1713:1741	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	3	44	from	kingianum	603:611	arg1	composition					503:513	monosaccharide composition	488:513	monosaccharide composition	488:513	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	44	from	kingianum	603:611	arg1	protein					516:522	protein	516:522	protein	516:522	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	44	from	kingianum	603:611	arg1	content					556:562	uronic acid content	544:562	uronic acid content	544:562	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	44	from	kingianum	603:611	arg1	weight					480:485	the molecular weight	466:485	the molecular weight	466:485	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	44	from	kingianum	603:611	arg1	polysaccharide					525:538	polysaccharide	525:538	polysaccharide	525:538	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	44	from	kingianum	603:611	arg1	polysaccharide					571:584	the polysaccharide	567:584	the polysaccharide from Polygonatum kingianum, respectively	567:625	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	8	45	from	expression	1404:1413	arg1	pathway					1446:1452	the LPS-TLR4/NFκB signaling pathway	1418:1452	the LPS-TLR4/NFκB signaling pathway	1418:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	3	46	theme	liquid	392:397	arg1	chromatography					399:412	liquid chromatography	392:412	liquid chromatography (HPLC)	392:419	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	46	theme	liquid	392:397	arg1	METHODS					343:349	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	46	theme	liquid	392:397	arg1	HPLC					415:418	HPLC	415:418	HPLC	415:418	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	10	47	theme	short	1713:1717	arg1	SCFA					1737:1740	SCFA	1737:1740	SCFA	1737:1740	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	10	47	theme	short	1713:1717	arg1	acid					1731:1734	short chain fatty acid	1713:1734	short chain fatty acid (SCFA)	1713:1741	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	12	48	theme	intestinal	2122:2131	arg1	barrier					2146:2152	the intestinal permeability barrier	2118:2152	the intestinal permeability barrier	2118:2152	This promotes recovery of the intestinal permeability barrier, inhibits LPS entry into the circulation, alleviates inflammation, and prevents glucose and lipid metabolic disorders.					
32028238	7	49	from	effect	1207:1212	arg1	content					1240:1246	the content	1236:1246	the content of SCFAs in the intestinal tract of rats	1236:1287	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	8	50	theme	blot	1479:1482	arg1	technique					1484:1492	Western blot technique	1471:1492	Western blot technique	1471:1492	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	8	51	theme	junction	1314:1321	arg1	ZO-1					1331:1334	tight junction protein ZO-1	1308:1334	tight junction protein ZO-1	1308:1334	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	9	52	dep	RESULTS	1495:1501	arg1	found					1506:1510	found	1506:1510	found that PS and PSF improved both diabetic symptoms and lipid metabolism	1506:1579	RESULTS We found that PS and PSF improved both diabetic symptoms and lipid metabolism.					
32028238	10	53	theme	increased	1766:1774	arg1	production					1781:1790	increased SCFA production	1766:1790	increased SCFA production	1766:1790	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	8	54	from	pathway	1446:1452	arg1	detection					1375:1383	detection	1375:1383	detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway	1375:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	8	54	from	pathway	1446:1452	arg1	expression					1294:1303	The expression	1290:1303	The expression of tight junction protein ZO-1 and occludin of intestinal tissue	1290:1368	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	3	55	theme	monosaccharide	488:501	arg1	composition					503:513	monosaccharide composition	488:513	monosaccharide composition	488:513	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	6	56	theme	measurement	1002:1012	arg1	method					1014:1019	rDNA V4 highly variable region measurement method	971:1019	rDNA V4 highly variable region measurement method	971:1019	The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora.					
32028238	3	57	from	composition	503:513	arg1	kingianum					603:611	Polygonatum kingianum	591:611	Polygonatum kingianum	591:611	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	58	from	protein	516:522	arg1	kingianum					603:611	Polygonatum kingianum	591:611	Polygonatum kingianum	591:611	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	2	59	theme	lipid	297:301	arg1	metabolism					303:312	lipid metabolism	297:312	lipid metabolism	297:312	Polysaccharides from Polygonatum kingianum could regulate glucose and lipid metabolism in high-fat diet (HFD) rats.					
32028238	11	60	theme	SCFA-producing	2067:2080	arg1	bacteria					2082:2089	SCFA-producing bacteria	2067:2089	SCFA-producing bacteria	2067:2089	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	4	61	theme	high	643:646	arg1	PSF					666:668	PSF	666:668	PSF	666:668	Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period.					
32028238	4	61	theme	high	643:646	arg1	weight					658:663	high molecular weight	643:663	high molecular weight (PSF) polysaccharides (> 100 KDa)	643:697	Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period.					
32028238	9	62	theme	lipid	1564:1568	arg1	metabolism					1570:1579	lipid metabolism	1564:1579	lipid metabolism	1564:1579	RESULTS We found that PS and PSF improved both diabetic symptoms and lipid metabolism.					
32028238	6	63	theme	intestinal	1067:1076	arg1	microflora					1078:1087	the intestinal microflora	1063:1087	the intestinal microflora	1063:1087	The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora.					
32028238	4	64	theme	weight	658:663	arg1	polysaccharides					671:685	high molecular weight (PSF) polysaccharides	643:685	high molecular weight (PSF) polysaccharides (> 100 KDa)	643:697	Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period.					
32028238	4	64	theme	weight	658:663	arg1	KDa					694:696	> 100 KDa	688:696	> 100 KDa	688:696	Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period.					
32028238	3	65	theme	acid	551:554	arg1	content					556:562	uronic acid content	544:562	uronic acid content	544:562	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	5	66	theme	blood	775:779	arg1	samples					781:787	blood samples	775:787	blood samples	775:787	TC, TG contents in blood samples were measured every two weeks, and HDL-C, LDL-C was measured at 12 and 14 weeks evaluating of blood lipids regulation activity of polysaccharide.					
32028238	8	67	theme	ZO-1	1331:1334	arg1	detection					1375:1383	detection	1375:1383	detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway	1375:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	8	67	theme	ZO-1	1331:1334	arg1	expression					1294:1303	The expression	1290:1303	The expression of tight junction protein ZO-1 and occludin of intestinal tissue	1290:1368	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	7	68	theme	rats	1284:1287	arg1	tract					1275:1279	the intestinal tract	1260:1279	the intestinal tract of rats	1260:1287	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	8	69	from	expression	1294:1303	arg1	pathway					1446:1452	the LPS-TLR4/NFκB signaling pathway	1418:1452	the LPS-TLR4/NFκB signaling pathway	1418:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	8	70	theme	occludin	1340:1347	arg1	detection					1375:1383	detection	1375:1383	detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway	1375:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	8	70	theme	occludin	1340:1347	arg1	expression					1294:1303	The expression	1290:1303	The expression of tight junction protein ZO-1 and occludin of intestinal tissue	1290:1368	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	7	71	theme	fatty	1117:1121	arg1	SCFAs					1129:1133	SCFAs	1129:1133	SCFAs	1129:1133	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	7	71	theme	fatty	1117:1121	arg1	acid					1123:1126	short chain fatty acid	1105:1126	short chain fatty acid (SCFAs)	1105:1134	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	3	72	theme	polysaccharide	571:584	arg1	weight					480:485	the molecular weight	466:485	the molecular weight	466:485	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	72	theme	polysaccharide	571:584	arg1	composition					503:513	monosaccharide composition	488:513	monosaccharide composition	488:513	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	72	theme	polysaccharide	571:584	arg1	content					556:562	uronic acid content	544:562	uronic acid content	544:562	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	72	theme	polysaccharide	571:584	arg1	polysaccharide					525:538	polysaccharide	525:538	polysaccharide	525:538	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	72	theme	polysaccharide	571:584	arg1	protein					516:522	protein	516:522	protein	516:522	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	11	73	theme	microbial	2002:2010	arg1	community					2012:2020	the intestinal microbial community	1987:2020	the intestinal microbial community	1987:2020	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	8	74	theme	intestinal	1352:1361	arg1	tissue					1363:1368	intestinal tissue	1352:1368	intestinal tissue	1352:1368	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	4	75	theme	>	688:688	arg1	polysaccharides					671:685	high molecular weight (PSF) polysaccharides	643:685	high molecular weight (PSF) polysaccharides (> 100 KDa)	643:697	Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period.					
32028238	4	75	theme	>	688:688	arg1	KDa					694:696	> 100 KDa	688:696	> 100 KDa	688:696	Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period.					
32028238	1	76	theme	Polygonatum	119:129	arg1	medicine					166:173	a traditional Chinese medicine	144:173	a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia	144:224	OBJECTIVE Polygonatum kingianum is a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia.					
32028238	1	76	theme	Polygonatum	119:129	arg1	kingianum					131:139	OBJECTIVE Polygonatum kingianum	109:139	OBJECTIVE Polygonatum kingianum	109:139	OBJECTIVE Polygonatum kingianum is a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia.					
32028238	2	77	theme	diet	326:329	arg1	rats					337:340	high-fat diet (HFD) rats	317:340	high-fat diet (HFD) rats	317:340	Polysaccharides from Polygonatum kingianum could regulate glucose and lipid metabolism in high-fat diet (HFD) rats.					
32028238	7	78	theme	SCFAs	1251:1255	arg1	content					1240:1246	the content	1236:1246	the content of SCFAs in the intestinal tract of rats	1236:1287	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	10	79	theme	relative	1691:1698	arg1	abundance					1700:1708	the relative abundance	1687:1708	the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production	1687:1790	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	2	80	from	metabolism	303:312	arg1	rats					337:340	high-fat diet (HFD) rats	317:340	high-fat diet (HFD) rats	317:340	Polysaccharides from Polygonatum kingianum could regulate glucose and lipid metabolism in high-fat diet (HFD) rats.					
32028238	6	81	theme	stool	939:943	arg1	samples					945:951	The stool samples	935:951	The stool samples	935:951	The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora.					
32028238	0	82	from	kingianum	33:41	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Polygonatum kingianum	0:41	Polysaccharides from Polygonatum kingianum improve glucose and lipid metabolism in rats fed a high fat diet.					
32028238	10	83	theme	lipid	1879:1883	arg1	metabolism					1885:1894	lipid metabolism	1879:1894	lipid metabolism	1879:1894	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	1	84	theme	traditional	146:156	arg1	medicine					166:173	a traditional Chinese medicine	144:173	a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia	144:224	OBJECTIVE Polygonatum kingianum is a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia.					
32028238	1	84	theme	traditional	146:156	arg1	kingianum					131:139	OBJECTIVE Polygonatum kingianum	109:139	OBJECTIVE Polygonatum kingianum	109:139	OBJECTIVE Polygonatum kingianum is a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia.					
32028238	12	85	theme	glucose	2234:2240	arg1	disorders					2262:2270	glucose and lipid metabolic disorders	2234:2270	glucose and lipid metabolic disorders	2234:2270	This promotes recovery of the intestinal permeability barrier, inhibits LPS entry into the circulation, alleviates inflammation, and prevents glucose and lipid metabolic disorders.					
32028238	0	86	theme	high	94:97	arg1	diet					103:106	a high fat diet	92:106	a high fat diet	92:106	Polysaccharides from Polygonatum kingianum improve glucose and lipid metabolism in rats fed a high fat diet.					
32028238	8	87	theme	protein	1396:1402	arg1	expression					1404:1413	the key protein expression	1388:1413	the key protein expression in the LPS-TLR4/NFκB signaling pathway	1388:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	12	88	theme	LPS	2164:2166	arg1	entry					2168:2172	LPS entry	2164:2172	LPS entry into the circulation	2164:2193	This promotes recovery of the intestinal permeability barrier, inhibits LPS entry into the circulation, alleviates inflammation, and prevents glucose and lipid metabolic disorders.					
32028238	4	89	theme	HFD	725:727	arg1	rats					729:732	HFD rats	725:732	HFD rats	725:732	Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period.					
32028238	7	90	theme	short	1105:1109	arg1	SCFAs					1129:1133	SCFAs	1129:1133	SCFAs	1129:1133	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	7	90	theme	short	1105:1109	arg1	acid					1123:1126	short chain fatty acid	1105:1126	short chain fatty acid (SCFAs)	1105:1134	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	7	91	from	content	1240:1246	arg1	tract					1275:1279	the intestinal tract	1260:1279	the intestinal tract of rats	1260:1287	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	7	92	theme	polysaccharides	1217:1231	arg1	effect					1207:1212	the effect	1203:1212	the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats	1203:1287	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	3	93	theme	gel	355:357	arg1	METHODS					343:349	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	93	theme	gel	355:357	arg1	GPC					386:388	GPC	386:388	GPC	386:388	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	93	theme	gel	355:357	arg1	chromatography					370:383	The gel permeation chromatography	351:383	The gel permeation chromatography (GPC)	351:389	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	7	94	from	content	1094:1100	arg1	faeces					1139:1144	faeces	1139:1144	faeces	1139:1144	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	8	95	theme	LPS-TLR4/NFκB	1422:1434	arg1	pathway					1446:1452	the LPS-TLR4/NFκB signaling pathway	1418:1452	the LPS-TLR4/NFκB signaling pathway	1418:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	12	96	theme	permeability	2133:2144	arg1	barrier					2146:2152	the intestinal permeability barrier	2118:2152	the intestinal permeability barrier	2118:2152	This promotes recovery of the intestinal permeability barrier, inhibits LPS entry into the circulation, alleviates inflammation, and prevents glucose and lipid metabolic disorders.					
32028238	11	97	theme	Polygonatum	1908:1918	arg1	CONCLUSION					1897:1906	CONCLUSION	1897:1906	CONCLUSION	1897:1906	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	8	98	from	detection	1375:1383	arg1	pathway					1446:1452	the LPS-TLR4/NFκB signaling pathway	1418:1452	the LPS-TLR4/NFκB signaling pathway	1418:1452	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	2	99	from	kingianum	260:268	arg1	Polysaccharides					227:241	Polysaccharides	227:241	Polysaccharides from Polygonatum kingianum	227:268	Polysaccharides from Polygonatum kingianum could regulate glucose and lipid metabolism in high-fat diet (HFD) rats.					
32028238	6	100	theme	rDNA	971:974	arg1	method					1014:1019	rDNA V4 highly variable region measurement method	971:1019	rDNA V4 highly variable region measurement method	971:1019	The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora.					
32028238	10	101	theme	acid	1731:1734	arg1	abundance					1700:1708	the relative abundance	1687:1708	the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production	1687:1790	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	0	102	theme	glucose	51:57	arg1	metabolism					69:78	glucose and lipid metabolism	51:78	metabolism	69:78	Polysaccharides from Polygonatum kingianum improve glucose and lipid metabolism in rats fed a high fat diet.					
32028238	6	103	theme	region	995:1000	arg1	method					1014:1019	rDNA V4 highly variable region measurement method	971:1019	rDNA V4 highly variable region measurement method	971:1019	The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora.					
32028238	3	104	dep	METHODS	343:349	arg1	analysis					434:441	chemical analysis	425:441	chemical analysis	425:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	104	dep	METHODS	343:349	arg1	chromatography					370:383	The gel permeation chromatography	351:383	The gel permeation chromatography (GPC)	351:389	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	104	dep	METHODS	343:349	arg1	chromatography					399:412	liquid chromatography	392:412	liquid chromatography (HPLC)	392:419	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	104	dep	METHODS	343:349	arg1	HPLC					415:418	HPLC	415:418	HPLC	415:418	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	104	dep	METHODS	343:349	arg1	METHODS					343:349	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis	343:441	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	3	104	dep	METHODS	343:349	arg1	GPC					386:388	GPC	386:388	GPC	386:388	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	10	105	theme	chain	1719:1723	arg1	SCFA					1737:1740	SCFA	1737:1740	SCFA	1737:1740	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	10	105	theme	chain	1719:1723	arg1	acid					1731:1734	short chain fatty acid	1713:1734	short chain fatty acid (SCFA)	1713:1741	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	6	106	from	role	1037:1040	arg1	microflora					1078:1087	the intestinal microflora	1063:1087	the intestinal microflora	1063:1087	The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora.					
32028238	0	107	theme	fat	99:101	arg1	diet					103:106	a high fat diet	92:106	a high fat diet	92:106	Polysaccharides from Polygonatum kingianum improve glucose and lipid metabolism in rats fed a high fat diet.					
32028238	0	108	theme	lipid	63:67	arg1	metabolism					69:78	glucose and lipid metabolism	51:78	metabolism	69:78	Polysaccharides from Polygonatum kingianum improve glucose and lipid metabolism in rats fed a high fat diet.					
32028238	6	109	theme	polysaccharide	1045:1058	arg1	role					1037:1040	the role	1033:1040	the role of polysaccharide in the intestinal microflora	1033:1087	The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora.					
32028238	8	110	theme	Western	1471:1477	arg1	blot					1479:1482	Western blot	1471:1482	Western blot technique	1471:1492	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
32028238	7	111	theme	gas	1164:1166	arg1	chromatography					1168:1181	gas chromatography	1164:1181	gas chromatography (GC)	1164:1186	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	7	111	theme	gas	1164:1166	arg1	GC					1184:1185	GC	1184:1185	GC	1184:1185	The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats.					
32028238	4	112	theme	14-week	740:746	arg1	period					748:753	a 14-week period	738:753	a 14-week period	738:753	Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period.					
32028238	3	113	theme	molecular	470:478	arg1	weight					480:485	the molecular weight	466:485	the molecular weight	466:485	METHODS The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively.					
32028238	5	114	theme	polysaccharide	919:932	arg1	activity					907:914	activity	907:914	activity of polysaccharide	907:932	TC, TG contents in blood samples were measured every two weeks, and HDL-C, LDL-C was measured at 12 and 14 weeks evaluating of blood lipids regulation activity of polysaccharide.					
32028238	11	115	dep	abundance	1960:1968	arg1	the					1956:1958	the	1956:1958	the	1956:1958	CONCLUSION Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria.					
32028238	10	116	theme	SCFA	1776:1779	arg1	production					1781:1790	increased SCFA production	1766:1790	increased SCFA production	1766:1790	PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.					
32028238	8	117	theme	tight	1308:1312	arg1	ZO-1					1331:1334	tight junction protein ZO-1	1308:1334	tight junction protein ZO-1	1308:1334	The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NFκB signaling pathway were detected by Western blot technique.					
34436307	3	0	theme	NF-κB	699:703	arg1	pathway					715:721	the NF-κB signaling pathway	695:721	the NF-κB signaling pathway	695:721	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	2	1	theme	RAW264.7	380:387	arg1	cells					389:393	lipopolysaccharide-stimulated RAW264.7 cells	350:393	lipopolysaccharide-stimulated RAW264.7 cells	350:393	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	4	2	theme	inflammatory	1001:1012	arg1	cytokines					1014:1022	inflammatory cytokines	1001:1022	inflammatory cytokines in serum	1001:1031	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	0	3	theme	DSS-Induced	83:93	arg1	Colitis					95:101	DSS-Induced Colitis	83:101	DSS-Induced Colitis in Mice	83:109	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice.					
34436307	0	4	from	Effects	18:24	arg1	Cells					73:77	RAW264.7 Cells	64:77	RAW264.7 Cells	64:77	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice.					
34436307	0	4	from	Effects	18:24	arg1	Colitis					95:101	DSS-Induced Colitis	83:101	DSS-Induced Colitis in Mice	83:109	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice.					
34436307	4	5	from	cytokines	1014:1022	arg1	serum					1027:1031	serum	1027:1031	serum	1027:1031	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	2	6	theme	lipopolysaccharide-stimulated	350:378	arg1	cells					389:393	lipopolysaccharide-stimulated RAW264.7 cells	350:393	lipopolysaccharide-stimulated RAW264.7 cells	350:393	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	4	7	from	effect	894:899	arg1	shortening					908:917	the shortening	904:917	the shortening of the colon caused by DSS	904:944	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	6	8	theme	probiotics	1335:1344	arg1	abundance					1317:1325	an increased abundance	1304:1325	an increased abundance of some probiotics like Anaerotruncus, Lactobacillus, Desulfovibrio, Alistipe, Odoribacter, and Enterorhabdus in colon	1304:1444	Specifically, mice treated with MP showed a repaired Firmicutes/Bacteroidetes ratio and an increased abundance of some probiotics like Anaerotruncus, Lactobacillus, Desulfovibrio, Alistipe, Odoribacter, and Enterorhabdus in colon.					
34436307	6	8	theme	probiotics	1335:1344	arg1	ratio					1294:1298	a repaired Firmicutes/Bacteroidetes ratio	1258:1298	a repaired Firmicutes/Bacteroidetes ratio	1258:1298	Specifically, mice treated with MP showed a repaired Firmicutes/Bacteroidetes ratio and an increased abundance of some probiotics like Anaerotruncus, Lactobacillus, Desulfovibrio, Alistipe, Odoribacter, and Enterorhabdus in colon.					
34436307	4	9	theme	disease	850:856	arg1	index					867:871	the disease activity index	846:871	the disease activity index	846:871	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	5	10	theme	community	1134:1142	arg1	diversity					1096:1104	diversity	1096:1104	diversity	1096:1104	Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora.					
34436307	5	10	theme	community	1134:1142	arg1	richness					1083:1090	richness	1083:1090	richness	1083:1090	Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora.					
34436307	3	11	theme	RAW264.7	558:565	arg1	cells					567:571	RAW264.7 cells	558:571	RAW264.7 cells	558:571	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	7	12	theme	promising	1489:1497	arg1	MP					1475:1476	the MP	1471:1476	the MP	1471:1476	These data suggest that the MP could be a promising dietary candidate for enhancing immunity and protecting against ulcerative colitis.					
34436307	7	12	theme	promising	1489:1497	arg1	candidate					1507:1515	a promising dietary candidate	1487:1515	a promising dietary candidate for enhancing immunity and protecting against ulcerative colitis	1487:1580	These data suggest that the MP could be a promising dietary candidate for enhancing immunity and protecting against ulcerative colitis.					
34436307	3	13	theme	cells	567:571	arg1	proliferation					541:553	the proliferation	537:553	the proliferation of RAW264.7 cells	537:571	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	2	14	theme	anti-inflammatory	282:298	arg1	effect					300:305	the anti-inflammatory effect	278:305	the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells	278:393	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	5	15	theme	structural	1168:1177	arg1	composition					1179:1189	the structural composition	1164:1189	the structural composition of the intestinal flora	1164:1213	Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora.					
34436307	2	16	dep	colitis	449:455	arg1	model					457:461	model	457:461	a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice	399:469	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	3	17	theme	signaling	705:713	arg1	pathway					715:721	the NF-κB signaling pathway	695:721	the NF-κB signaling pathway	695:721	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	4	18	theme	weight	818:823	arg1	loss					825:828	the weight loss	814:828	the weight loss of mice	814:836	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	4	19	theme	barrier	970:976	arg1	integrity					978:986	intestinal barrier integrity	959:986	intestinal barrier integrity	959:986	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	3	20	theme	pathway	715:721	arg1	activation					681:690	the activation	677:690	the activation of the NF-κB signaling pathway	677:721	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	4	21	theme	mice	833:836	arg1	loss					825:828	the weight loss	814:828	the weight loss of mice	814:836	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	5	22	dep	richness	1083:1090	arg1	the					1079:1081	the	1079:1081	the	1079:1081	Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora.					
34436307	2	23	theme	polysaccharide	327:340	arg1	effect					300:305	the anti-inflammatory effect	278:305	the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells	278:393	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	2	23	theme	polysaccharide	327:340	arg1	colitis					449:455	a dextran sulfate sodium (DSS)-induced ulcerative colitis	399:455	a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice	399:469	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	6	24	theme	repaired	1260:1267	arg1	ratio					1294:1298	a repaired Firmicutes/Bacteroidetes ratio	1258:1298	a repaired Firmicutes/Bacteroidetes ratio	1258:1298	Specifically, mice treated with MP showed a repaired Firmicutes/Bacteroidetes ratio and an increased abundance of some probiotics like Anaerotruncus, Lactobacillus, Desulfovibrio, Alistipe, Odoribacter, and Enterorhabdus in colon.					
34436307	1	25	theme	critical	188:195	arg1	role					197:200	a critical role	186:200	a critical role	186:200	Considerable literature has been published on polysaccharides, which play a critical role in regulating the pathogenesis of inflammation and immunity.					
34436307	0	26	theme	Anti-Inflammatory	0:16	arg1	Effects					18:24	Anti-Inflammatory Effects	0:24	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice	0:109	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice.					
34436307	5	27	theme	microbial	1124:1132	arg1	community					1134:1142	the intestinal microbial community	1109:1142	the intestinal microbial community	1109:1142	Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora.					
34436307	4	28	from	colitis	740:746	arg1	mice					751:754	mice	751:754	mice	751:754	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	2	29	theme	coruscus	318:325	arg1	MP					343:344	MP	343:344	MP	343:344	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	2	29	theme	coruscus	318:325	arg1	polysaccharide					327:340	Mytilus coruscus polysaccharide	310:340	Mytilus coruscus polysaccharide (MP)	310:345	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	3	30	dep	cytokines	627:635	arg1	IL-10					655:659	IL-10	655:659	IL-10	655:659	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	3	30	dep	cytokines	627:635	arg1	TNF-α					638:642	TNF-α	638:642	TNF-α	638:642	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	3	30	dep	cytokines	627:635	arg1	cytokines					627:635	inflammatory cytokines	614:635	inflammatory cytokines (TNF-α, IL-6, and IL-10)	614:660	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	3	30	dep	cytokines	627:635	arg1	IL-6					645:648	IL-6	645:648	IL-6	645:648	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	0	31	theme	Mytilus	29:35	arg1	Polysaccharide					46:59	Mytilus coruscus Polysaccharide	29:59	Mytilus coruscus Polysaccharide	29:59	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice.					
34436307	4	32	theme	DSS-induced	728:738	arg1	colitis					740:746	DSS-induced colitis	728:746	DSS-induced colitis in mice	728:754	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	6	33	theme	increased	1307:1315	arg1	abundance					1317:1325	an increased abundance	1304:1325	an increased abundance of some probiotics like Anaerotruncus, Lactobacillus, Desulfovibrio, Alistipe, Odoribacter, and Enterorhabdus in colon	1304:1444	Specifically, mice treated with MP showed a repaired Firmicutes/Bacteroidetes ratio and an increased abundance of some probiotics like Anaerotruncus, Lactobacillus, Desulfovibrio, Alistipe, Odoribacter, and Enterorhabdus in colon.					
34436307	2	34	theme	Mytilus	310:316	arg1	MP					343:344	MP	343:344	MP	343:344	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	2	34	theme	Mytilus	310:316	arg1	polysaccharide					327:340	Mytilus coruscus polysaccharide	310:340	Mytilus coruscus polysaccharide (MP)	310:345	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	2	35	theme	-induced	429:436	arg1	colitis					449:455	a dextran sulfate sodium (DSS)-induced ulcerative colitis	399:455	a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice	399:469	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	2	36	theme	ulcerative	438:447	arg1	colitis					449:455	a dextran sulfate sodium (DSS)-induced ulcerative colitis	399:455	a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice	399:469	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	0	37	from	Colitis	95:101	arg1	Mice					106:109	Mice	106:109	Mice	106:109	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice.					
34436307	1	38	theme	Considerable	112:123	arg1	literature					125:134	Considerable literature	112:134	Considerable literature	112:134	Considerable literature has been published on polysaccharides, which play a critical role in regulating the pathogenesis of inflammation and immunity.					
34436307	4	39	contain	have	878:881	arg2	effect					894:899	a positive effect	883:899	a positive effect on the shortening of the colon caused by DSS	883:944	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	4	39	contain	have	878:881	arg1	MP					757:758	MP	757:758	MP	757:758	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	0	40	theme	Polysaccharide	46:59	arg1	Effects					18:24	Anti-Inflammatory Effects	0:24	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice	0:109	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice.					
34436307	7	41	theme	dietary	1499:1505	arg1	MP					1475:1476	the MP	1471:1476	the MP	1471:1476	These data suggest that the MP could be a promising dietary candidate for enhancing immunity and protecting against ulcerative colitis.					
34436307	7	41	theme	dietary	1499:1505	arg1	candidate					1507:1515	a promising dietary candidate	1487:1515	a promising dietary candidate for enhancing immunity and protecting against ulcerative colitis	1487:1580	These data suggest that the MP could be a promising dietary candidate for enhancing immunity and protecting against ulcerative colitis.					
34436307	3	42	theme	inflammatory	614:625	arg1	IL-10					655:659	IL-10	655:659	IL-10	655:659	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	3	42	theme	inflammatory	614:625	arg1	TNF-α					638:642	TNF-α	638:642	TNF-α	638:642	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	3	42	theme	inflammatory	614:625	arg1	cytokines					627:635	inflammatory cytokines	614:635	inflammatory cytokines (TNF-α, IL-6, and IL-10)	614:660	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	3	42	theme	inflammatory	614:625	arg1	IL-6					645:648	IL-6	645:648	IL-6	645:648	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	4	43	theme	positive	885:892	arg1	effect					894:899	a positive effect	883:899	a positive effect on the shortening of the colon caused by DSS	883:944	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	3	44	theme	cytokines	627:635	arg1	production					600:609	the excessive production	586:609	the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10)	586:660	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	0	45	theme	RAW264.7	64:71	arg1	Cells					73:77	RAW264.7 Cells	64:77	RAW264.7 Cells	64:77	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice.					
34436307	6	46	theme	Firmicutes/Bacteroidetes	1269:1292	arg1	ratio					1294:1298	a repaired Firmicutes/Bacteroidetes ratio	1258:1298	a repaired Firmicutes/Bacteroidetes ratio	1258:1298	Specifically, mice treated with MP showed a repaired Firmicutes/Bacteroidetes ratio and an increased abundance of some probiotics like Anaerotruncus, Lactobacillus, Desulfovibrio, Alistipe, Odoribacter, and Enterorhabdus in colon.					
34436307	0	47	from	Cells	73:77	arg1	Mice					106:109	Mice	106:109	Mice	106:109	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice.					
34436307	5	48	theme	notable	1055:1061	arg1	contribution					1063:1074	a notable contribution	1053:1074	a notable contribution to the richness and diversity of the intestinal microbial community	1053:1142	Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora.					
34436307	4	49	theme	activity	858:865	arg1	index					867:871	the disease activity index	846:871	the disease activity index	846:871	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	6	50	from	Desulfovibrio	1381:1393	arg1	colon					1440:1444	colon	1440:1444	colon	1440:1444	Specifically, mice treated with MP showed a repaired Firmicutes/Bacteroidetes ratio and an increased abundance of some probiotics like Anaerotruncus, Lactobacillus, Desulfovibrio, Alistipe, Odoribacter, and Enterorhabdus in colon.					
34436307	5	51	theme	intestinal	1113:1122	arg1	community					1134:1142	the intestinal microbial community	1109:1142	the intestinal microbial community	1109:1142	Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora.					
34436307	4	52	theme	clinical	776:783	arg1	symptoms					785:792	the clinical symptoms	772:792	the clinical symptoms of colitis	772:803	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	0	53	dep	Mytilus	29:35	arg1	coruscus					37:44	coruscus	37:44	coruscus	37:44	Anti-Inflammatory Effects of Mytilus coruscus Polysaccharide on RAW264.7 Cells and DSS-Induced Colitis in Mice.					
34436307	4	54	theme	colitis	797:803	arg1	symptoms					785:792	the clinical symptoms	772:792	the clinical symptoms of colitis	772:803	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	7	55	theme	ulcerative	1563:1572	arg1	colitis					1574:1580	ulcerative colitis	1563:1580	ulcerative colitis	1563:1580	These data suggest that the MP could be a promising dietary candidate for enhancing immunity and protecting against ulcerative colitis.					
34436307	2	56	from	effect	300:305	arg1	cells					389:393	lipopolysaccharide-stimulated RAW264.7 cells	350:393	lipopolysaccharide-stimulated RAW264.7 cells	350:393	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	2	56	from	effect	300:305	arg1	mice					466:469	mice	466:469	mice	466:469	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	1	57	theme	inflammation	236:247	arg1	pathogenesis					220:231	the pathogenesis	216:231	the pathogenesis of inflammation and immunity	216:260	Considerable literature has been published on polysaccharides, which play a critical role in regulating the pathogenesis of inflammation and immunity.					
34436307	4	58	theme	colon	926:930	arg1	shortening					908:917	the shortening	904:917	the shortening of the colon caused by DSS	904:944	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	4	59	theme	intestinal	959:968	arg1	integrity					978:986	intestinal barrier integrity	959:986	intestinal barrier integrity	959:986	For DSS-induced colitis in mice, MP can improve the clinical symptoms of colitis, inhibit the weight loss of mice, reduce the disease activity index, and have a positive effect on the shortening of the colon caused by DSS, meliorating intestinal barrier integrity and lowering inflammatory cytokines in serum.					
34436307	2	60	from	colitis	449:455	arg1	cells					389:393	lipopolysaccharide-stimulated RAW264.7 cells	350:393	lipopolysaccharide-stimulated RAW264.7 cells	350:393	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	2	60	from	colitis	449:455	arg1	mice					466:469	mice	466:469	mice	466:469	In this essay, the anti-inflammatory effect of Mytilus coruscus polysaccharide (MP) on lipopolysaccharide-stimulated RAW264.7 cells and a dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice was investigated.					
34436307	5	61	theme	intestinal	1198:1207	arg1	flora					1209:1213	the intestinal flora	1194:1213	the intestinal flora	1194:1213	Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora.					
34436307	3	62	theme	excessive	590:598	arg1	production					600:609	the excessive production	586:609	the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10)	586:660	The results showed that MP effectively promoted the proliferation of RAW264.7 cells, ameliorated the excessive production of inflammatory cytokines (TNF-α, IL-6, and IL-10), and inhibited the activation of the NF-κB signaling pathway.					
34436307	5	63	theme	flora	1209:1213	arg1	composition					1179:1189	the structural composition	1164:1189	the structural composition of the intestinal flora	1164:1213	Moreover, MP makes a notable contribution to the richness and diversity of the intestinal microbial community, and also regulates the structural composition of the intestinal flora.					
34436307	1	64	theme	immunity	253:260	arg1	pathogenesis					220:231	the pathogenesis	216:231	the pathogenesis of inflammation and immunity	216:260	Considerable literature has been published on polysaccharides, which play a critical role in regulating the pathogenesis of inflammation and immunity.					
32216024	7	0	theme	free	1448:1451	arg1	substance					1477:1485	a free extracellular polymeric substance	1446:1485	a free extracellular polymeric substance (was arbitrarily called fEPS)	1446:1515	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	4	1	theme	H.	894:895	arg1	P6-12					907:911	H. lusitanum P6-12	894:911	H. lusitanum P6-12	894:911	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	10	2	theme	H.	1998:1999	arg1	surface					2017:2023	the H. lusitanum P6-12 surface	1994:2023	the H. lusitanum P6-12 surface	1994:2023	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	8	3	theme	high-molecular-weight	1638:1658	arg1	glycoconjugate					1660:1673	high-molecular-weight glycoconjugate	1638:1673	high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%)	1638:1795	The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%).					
32216024	2	4	theme	gas-liquid	581:590	arg1	chromatography					592:605	gas-liquid chromatography	581:605	gas-liquid chromatography	581:605	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	6	5	theme	extracellular	1271:1283	arg1	EPM					1303:1305	EPM	1303:1305	EPM	1303:1305	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	5	theme	extracellular	1271:1283	arg1	matrix					1295:1300	The extracellular polymeric matrix	1267:1300	The extracellular polymeric matrix (EPM) produced by the biofilm cells	1267:1336	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	5	theme	extracellular	1271:1283	arg1	proteinaceous					1349:1361	proteinaceous	1349:1361	proteinaceous	1349:1361	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	7	6	theme	culture	1430:1436	arg1	medium					1438:1443	the biofilm culture medium	1418:1443	the biofilm culture medium	1418:1443	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	10	7	dep	H.	1998:1999	arg1	lusitanum					2001:2009	H. lusitanum	1998:2009	the H. lusitanum P6-12 surface	1994:2023	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	8	8	dep	16	1769:1770	arg1	to					1766:1767	to	1766:1767	to	1766:1767	The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%).					
32216024	3	9	theme	column	724:729	arg1	chromatography					731:744	column chromatography	724:744	column chromatography	724:744	A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum.					
32216024	4	10	theme	complex	1152:1158	arg1	glycoconjugates					1030:1044	two high-molecular-weight glycoconjugates	1004:1044	two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS)	1004:1188	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	1	11	from	swimming	265:272	arg1	which					244:248	which	244:248	which	244:248	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	2	12	theme	sodium	521:526	arg1	electrophoresis					564:578	sodium dodecyl sulphate-polyacrylamide gel electrophoresis	521:578	sodium dodecyl sulphate-polyacrylamide gel electrophoresis	521:578	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	11	13	theme	physiological	2301:2313	arg1	properties					2315:2324	the physiological properties	2297:2324	the physiological properties of the biopolymers	2297:2343	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study permit a better understanding of the physiological properties of the biopolymers, for example, in plant-microbe interactions.					
32216024	8	14	contain	containing	1627:1636	arg1	capsules					1618:1625	capsules	1618:1625	capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%)	1618:1795	The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%).					
32216024	8	14	contain	containing	1627:1636	arg2	glycoconjugate					1660:1673	high-molecular-weight glycoconjugate	1638:1673	high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%)	1638:1795	The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%).					
32216024	1	15	theme	Herbaspirillum	190:203	arg1	growth					221:226	the Herbaspirillum lusitanum P6-12 growth	186:226	the Herbaspirillum lusitanum P6-12 growth	186:226	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	4	16	theme	nature	1119:1124	arg1	glycoconjugates					1030:1044	two high-molecular-weight glycoconjugates	1004:1044	two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS)	1004:1188	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	8	17	contain	had	1614:1616	arg1	cells					1588:1592	The cells	1584:1592	The cells outside the biofilm	1584:1612	The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%).					
32216024	8	17	contain	had	1614:1616	arg2	capsules					1618:1625	capsules	1618:1625	capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%)	1618:1795	The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%).					
32216024	4	18	theme	called	1064:1069	arg1	CPS-I					1071:1075	CPS-I	1071:1075	CPS-I	1071:1075	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	1	19	theme	P6-12	215:219	arg1	growth					221:226	the Herbaspirillum lusitanum P6-12 growth	186:226	the Herbaspirillum lusitanum P6-12 growth	186:226	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	9	20	theme	secreted	1849:1856	arg1	formation					1825:1833	biofilm formation	1817:1833	biofilm formation	1817:1833	CONCLUSIONS During biofilm formation, the bacteria secreted surface biopolymers that differed from those of the planktonic cells.					
32216024	9	20	theme	secreted	1849:1856	arg1	biopolymers					1866:1876	the bacteria secreted surface biopolymers	1836:1876	the bacteria secreted surface biopolymers that differed from those of the planktonic cells	1836:1925	CONCLUSIONS During biofilm formation, the bacteria secreted surface biopolymers that differed from those of the planktonic cells.					
32216024	4	21	theme	P6-12	907:911	arg1	cells					885:889	planktonic cells	874:889	planktonic cells of H. lusitanum P6-12	874:911	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	2	22	theme	sulphate-polyacrylamide	536:558	arg1	electrophoresis					564:578	sodium dodecyl sulphate-polyacrylamide gel electrophoresis	521:578	sodium dodecyl sulphate-polyacrylamide gel electrophoresis	521:578	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	11	23	theme	properties	2315:2324	arg1	understanding					2280:2292	a better understanding	2271:2292	a better understanding of the physiological properties of the biopolymers	2271:2343	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study permit a better understanding of the physiological properties of the biopolymers, for example, in plant-microbe interactions.					
32216024	3	24	theme	planktonic	831:840	arg1	lusitanum					845:853	planktonic H. lusitanum	831:853	planktonic H. lusitanum	831:853	A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum.					
32216024	3	25	theme	high	632:635	arg1	degree					637:642	A high degree	630:642	A high degree of polymer separation and purification	630:681	A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum.					
32216024	6	26	with	proteinaceous	1349:1361	arg1	%					1409:1409	up to 3%	1402:1409	up to 3%	1402:1409	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	26	with	proteinaceous	1349:1361	arg1	carbohydrates					1387:1399	carbohydrates	1387:1399	carbohydrates (up to 3%)	1387:1410	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	26	with	proteinaceous	1349:1361	arg1	amount					1377:1382	a small amount	1369:1382	a small amount of carbohydrates (up to 3%)	1369:1410	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	1	27	theme	study	130:134	arg1	goal					117:120	The goal	113:120	The goal of this study	113:134	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	1	28	dep	AIMS	108:111	arg1	was					136:138	was	136:138	was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile	136:328	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	0	29	theme	biopolymers	20:30	arg1	Characterization					0:15	Characterization	0:15	Characterization of biopolymers	0:30	Characterization of biopolymers produced by planktonic and biofilm cells of Herbaspirillum lusitanum P6-12.					
32216024	5	30	dep	EPS	1195:1197	arg1	CPS-I					1200:1204	CPS-I	1200:1204	CPS-I	1200:1204	The EPS, CPS-I, CPS-II had different monosaccharide and lipid compositions.					
32216024	4	31	theme	called	1178:1183	arg1	EPS					1185:1187	arbitrarily called EPS	1166:1187	arbitrarily called EPS	1166:1187	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	0	32	theme	Herbaspirillum	76:89	arg1	P6-12					101:105	Herbaspirillum lusitanum P6-12	76:105	Herbaspirillum lusitanum P6-12	76:105	Characterization of biopolymers produced by planktonic and biofilm cells of Herbaspirillum lusitanum P6-12.					
32216024	4	33	theme	lusitanum	897:905	arg1	P6-12					907:911	H. lusitanum P6-12	894:911	H. lusitanum P6-12	894:911	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	2	34	from	cells	418:422	arg1	Differences					351:361	METHODS AND RESULTS Differences	331:361	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12	331:451	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	2	34	from	cells	418:422	arg1	composition					378:388	biopolymers composition	366:388	biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12	366:451	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	0	35	theme	P6-12	101:105	arg1	cells					67:71	planktonic and biofilm cells	44:71	planktonic and biofilm cells of Herbaspirillum lusitanum P6-12	44:105	Characterization of biopolymers produced by planktonic and biofilm cells of Herbaspirillum lusitanum P6-12.					
32216024	4	36	theme	exponential	966:976	arg1	phase					978:982	the exponential phase	962:982	the exponential phase of growth	962:992	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	10	37	theme	efficient	2120:2128	arg1	symbiosis					2130:2138	an efficient symbiosis	2117:2138	an efficient symbiosis	2117:2138	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	2	38	theme	RESULTS	343:349	arg1	Differences					351:361	METHODS AND RESULTS Differences	331:361	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12	331:451	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	1	39	theme	free	260:263	arg1	cells					250:254	cells	250:254	cells	250:254	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	1	39	theme	free	260:263	arg1	swimming					265:272	free swimming	260:272	free swimming	260:272	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	2	40	theme	biofilm	410:416	arg1	cells					418:422	planktonic and biofilm cells	395:422	planktonic and biofilm cells	395:422	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	3	41	from	lusitanum	845:853	arg1	biopolymers					802:812	biopolymers	802:812	biopolymers from biofilm and planktonic H. lusitanum	802:853	A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum.					
32216024	8	42	dep	glycoconjugate	1660:1673	arg1	CPSFBC					1699:1704	arbitrarily called CPSFBC	1680:1704	arbitrarily called CPSFBC	1680:1704	The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%).					
32216024	2	43	theme	planktonic	395:404	arg1	cells					418:422	planktonic and biofilm cells	395:422	planktonic and biofilm cells	395:422	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	1	44	from	sessile	322:328	arg1	modes					177:181	two modes	173:181	two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style	173:296	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	1	44	from	sessile	322:328	arg1	biopolymers					156:166	biopolymers	156:166	biopolymers	156:166	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	2	45	dep	transform	481:489	arg1	infrared					491:498	infrared	491:498	transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry	481:627	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	7	46	theme	called	1504:1509	arg1	fEPS					1511:1514	arbitrarily called fEPS	1492:1514	arbitrarily called fEPS	1492:1514	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	10	47	from	existence	2174:2182	arg1	tissues					2193:2199	plant tissues	2187:2199	plant tissues	2187:2199	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	3	48	theme	separation	655:664	arg1	degree					637:642	A high degree	630:642	A high degree of polymer separation and purification	630:681	A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum.					
32216024	1	49	dep	modes	177:181	arg1	planktonic					229:238	planktonic	229:238	planktonic	229:238	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	1	49	dep	modes	177:181	arg1	style					292:296	biofilm life style	279:296	biofilm life style	279:296	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	1	50	theme	biofilm	279:285	arg1	style					292:296	biofilm life style	279:296	biofilm life style	279:296	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	2	51	theme	lusitanum	430:438	arg1	strain					440:445	H. lusitanum strain P6-12	427:451	H. lusitanum strain P6-12	427:451	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	3	52	from	biofilm	819:825	arg1	biopolymers					802:812	biopolymers	802:812	biopolymers from biofilm and planktonic H. lusitanum	802:853	A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum.					
32216024	8	53	dep	70	1792:1793	arg1	to					1789:1790	to	1789:1790	to	1789:1790	The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%).					
32216024	6	54	theme	carbohydrates	1387:1399	arg1	%					1409:1409	up to 3%	1402:1409	up to 3%	1402:1409	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	54	theme	carbohydrates	1387:1399	arg1	carbohydrates					1387:1399	carbohydrates	1387:1399	carbohydrates (up to 3%)	1387:1410	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	54	theme	carbohydrates	1387:1399	arg1	amount					1377:1382	a small amount	1369:1382	a small amount of carbohydrates (up to 3%)	1369:1410	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	55	dep	3	1408:1408	arg1	to					1405:1406	to	1405:1406	to	1405:1406	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	10	56	theme	biopolymers	1979:1989	arg1	heterogeneity					1932:1944	The heterogeneity	1928:1944	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface	1928:2023	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	1	57	theme	growth	221:226	arg1	modes					177:181	two modes	173:181	two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style	173:296	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	7	58	theme	extracellular	1453:1465	arg1	substance					1477:1485	a free extracellular polymeric substance	1446:1485	a free extracellular polymeric substance (was arbitrarily called fEPS)	1446:1515	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	7	59	contain	contained	1535:1543	arg2	carbohydrates					1558:1570	carbohydrates	1558:1570	carbohydrates (up to 7%)	1558:1581	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	7	59	contain	contained	1535:1543	arg2	proteins					1545:1552	proteins	1545:1552	proteins	1545:1552	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	7	59	contain	contained	1535:1543	arg1	that					1530:1533	that	1530:1533	that	1530:1533	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	7	59	contain	contained	1535:1543	arg2	%					1580:1580	up to 7%	1573:1580	up to 7%	1573:1580	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	10	60	theme	polysaccharide	1953:1966	arg1	biopolymers					1979:1989	the polysaccharide containing biopolymers	1949:1989	the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface	1949:2023	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	3	61	theme	purification	670:681	arg1	degree					637:642	A high degree	630:642	A high degree of polymer separation and purification	630:681	A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum.					
32216024	2	62	theme	METHODS	331:337	arg1	Differences					351:361	METHODS AND RESULTS Differences	331:361	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12	331:451	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	6	63	theme	small	1371:1375	arg1	%					1409:1409	up to 3%	1402:1409	up to 3%	1402:1409	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	63	theme	small	1371:1375	arg1	carbohydrates					1387:1399	carbohydrates	1387:1399	carbohydrates (up to 3%)	1387:1410	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	63	theme	small	1371:1375	arg1	amount					1377:1382	a small amount	1369:1382	a small amount of carbohydrates (up to 3%)	1369:1410	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	11	64	dep	SIGNIFICANCE	2202:2213	arg1	results					2243:2249	The results	2239:2249	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study	2202:2262	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study permit a better understanding of the physiological properties of the biopolymers, for example, in plant-microbe interactions.					
32216024	11	65	theme	STUDY	2233:2237	arg1	IMPACT					2219:2224	IMPACT	2219:2224	IMPACT	2219:2224	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study permit a better understanding of the physiological properties of the biopolymers, for example, in plant-microbe interactions.					
32216024	11	65	theme	STUDY	2233:2237	arg1	SIGNIFICANCE					2202:2213	SIGNIFICANCE	2202:2213	SIGNIFICANCE	2202:2213	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study permit a better understanding of the physiological properties of the biopolymers, for example, in plant-microbe interactions.					
32216024	6	66	theme	polymeric	1285:1293	arg1	EPM					1303:1305	EPM	1303:1305	EPM	1303:1305	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	66	theme	polymeric	1285:1293	arg1	matrix					1295:1300	The extracellular polymeric matrix	1267:1300	The extracellular polymeric matrix (EPM) produced by the biofilm cells	1267:1336	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	6	66	theme	polymeric	1285:1293	arg1	proteinaceous					1349:1361	proteinaceous	1349:1361	proteinaceous	1349:1361	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	7	67	dep	substance	1477:1485	arg1	fEPS					1511:1514	arbitrarily called fEPS	1492:1514	arbitrarily called fEPS	1492:1514	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	7	68	theme	biofilm	1422:1428	arg1	medium					1438:1443	the biofilm culture medium	1418:1443	the biofilm culture medium	1418:1443	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	5	69	contain	had	1214:1216	arg2	monosaccharide					1228:1241	different monosaccharide and lipid compositions	1218:1264	monosaccharide	1228:1241	The EPS, CPS-I, CPS-II had different monosaccharide and lipid compositions.					
32216024	5	69	contain	had	1214:1216	arg1	CPS-II					1207:1212	The EPS, CPS-I, CPS-II	1191:1212	The EPS, CPS-I, CPS-II	1191:1212	The EPS, CPS-I, CPS-II had different monosaccharide and lipid compositions.					
32216024	5	69	contain	had	1214:1216	arg2	compositions					1253:1264	different monosaccharide and lipid compositions	1218:1264	compositions	1253:1264	The EPS, CPS-I, CPS-II had different monosaccharide and lipid compositions.					
32216024	4	70	theme	lipid-polysacharide	1132:1150	arg1	complex					1152:1158	a lipid-polysacharide complex	1130:1158	a lipid-polysacharide complex (were arbitrarily called EPS)	1130:1188	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	10	71	theme	plant	2081:2085	arg1	colonization					2087:2098	plant colonization	2081:2098	plant colonization	2081:2098	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	2	72	theme	H.	427:428	arg1	strain					440:445	H. lusitanum strain P6-12	427:451	H. lusitanum strain P6-12	427:451	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	10	73	theme	surface	2017:2023	arg1	biopolymers					1979:1989	the polysaccharide containing biopolymers	1949:1989	the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface	1949:2023	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	10	74	from	functions	2068:2076	arg1	adaptation					2160:2169	cell adaptation	2155:2169	cell adaptation to existence in plant tissues	2155:2199	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	10	74	from	functions	2068:2076	arg1	colonization					2087:2098	plant colonization	2081:2098	plant colonization	2081:2098	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	4	75	theme	planktonic	874:883	arg1	cells					885:889	planktonic cells	874:889	planktonic cells of H. lusitanum P6-12	874:911	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	9	76	theme	biofilm	1817:1823	arg1	formation					1825:1833	biofilm formation	1817:1833	biofilm formation	1817:1833	CONCLUSIONS During biofilm formation, the bacteria secreted surface biopolymers that differed from those of the planktonic cells.					
32216024	9	76	theme	biofilm	1817:1823	arg1	biopolymers					1866:1876	the bacteria secreted surface biopolymers	1836:1876	the bacteria secreted surface biopolymers that differed from those of the planktonic cells	1836:1925	CONCLUSIONS During biofilm formation, the bacteria secreted surface biopolymers that differed from those of the planktonic cells.					
32216024	3	77	theme	chemical	773:780	arg1	differences					782:792	the chemical differences	769:792	the chemical differences between biopolymers from biofilm and planktonic H. lusitanum	769:853	A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum.					
32216024	8	78	theme	called	1692:1697	arg1	CPSFBC					1699:1704	arbitrarily called CPSFBC	1680:1704	arbitrarily called CPSFBC	1680:1704	The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%).					
32216024	4	79	theme	liquid	934:939	arg1	medium					941:946	a liquid medium	932:946	a liquid medium to the end of the exponential phase of growth	932:992	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	1	80	dep	planktonic	229:238	arg1	cells					250:254	cells	250:254	cells	250:254	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	1	80	dep	planktonic	229:238	arg1	swimming					265:272	free swimming	260:272	free swimming	260:272	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	10	81	theme	different	2058:2066	arg1	functions					2068:2076	their different functions	2052:2076	their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues	2052:2199	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	6	82	theme	biofilm	1324:1330	arg1	cells					1332:1336	the biofilm cells	1320:1336	the biofilm cells	1320:1336	The extracellular polymeric matrix (EPM) produced by the biofilm cells was mostly proteinaceous, with a small amount of carbohydrates (up to 3%).					
32216024	9	83	theme	bacteria	1840:1847	arg1	formation					1825:1833	biofilm formation	1817:1833	biofilm formation	1817:1833	CONCLUSIONS During biofilm formation, the bacteria secreted surface biopolymers that differed from those of the planktonic cells.					
32216024	9	83	theme	bacteria	1840:1847	arg1	biopolymers					1866:1876	the bacteria secreted surface biopolymers	1836:1876	the bacteria secreted surface biopolymers that differed from those of the planktonic cells	1836:1925	CONCLUSIONS During biofilm formation, the bacteria secreted surface biopolymers that differed from those of the planktonic cells.					
32216024	2	84	theme	gel	560:562	arg1	electrophoresis					564:578	sodium dodecyl sulphate-polyacrylamide gel electrophoresis	521:578	sodium dodecyl sulphate-polyacrylamide gel electrophoresis	521:578	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	9	85	theme	surface	1858:1864	arg1	formation					1825:1833	biofilm formation	1817:1833	biofilm formation	1817:1833	CONCLUSIONS During biofilm formation, the bacteria secreted surface biopolymers that differed from those of the planktonic cells.					
32216024	9	85	theme	surface	1858:1864	arg1	biopolymers					1866:1876	the bacteria secreted surface biopolymers	1836:1876	the bacteria secreted surface biopolymers that differed from those of the planktonic cells	1836:1925	CONCLUSIONS During biofilm formation, the bacteria secreted surface biopolymers that differed from those of the planktonic cells.					
32216024	2	86	theme	dodecyl	528:534	arg1	electrophoresis					564:578	sodium dodecyl sulphate-polyacrylamide gel electrophoresis	521:578	sodium dodecyl sulphate-polyacrylamide gel electrophoresis	521:578	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	3	87	theme	H.	842:843	arg1	lusitanum					845:853	planktonic H. lusitanum	831:853	planktonic H. lusitanum	831:853	A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum.					
32216024	10	88	theme	containing	1968:1977	arg1	biopolymers					1979:1989	the polysaccharide containing biopolymers	1949:1989	the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface	1949:2023	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	4	89	theme	phase	978:982	arg1	end					955:957	the end	951:957	the end of the exponential phase of growth	951:992	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	11	90	theme	plant-microbe	2362:2374	arg1	interactions					2376:2387	plant-microbe interactions	2362:2387	plant-microbe interactions	2362:2387	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study permit a better understanding of the physiological properties of the biopolymers, for example, in plant-microbe interactions.					
32216024	11	91	theme	biopolymers	2333:2343	arg1	properties					2315:2324	the physiological properties	2297:2324	the physiological properties of the biopolymers	2297:2343	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study permit a better understanding of the physiological properties of the biopolymers, for example, in plant-microbe interactions.					
32216024	5	92	theme	lipid	1247:1251	arg1	compositions					1253:1264	different monosaccharide and lipid compositions	1218:1264	compositions	1253:1264	The EPS, CPS-I, CPS-II had different monosaccharide and lipid compositions.					
32216024	0	93	theme	lusitanum	91:99	arg1	P6-12					101:105	Herbaspirillum lusitanum P6-12	76:105	Herbaspirillum lusitanum P6-12	76:105	Characterization of biopolymers produced by planktonic and biofilm cells of Herbaspirillum lusitanum P6-12.					
32216024	4	94	theme	growth	987:992	arg1	phase					978:982	the exponential phase	962:982	the exponential phase of growth	962:992	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	10	95	theme	symbiosis	2130:2138	arg1	formation					2104:2112	formation	2104:2112	formation of an efficient symbiosis	2104:2138	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	9	96	theme	planktonic	1910:1919	arg1	cells					1921:1925	the planktonic cells	1906:1925	the planktonic cells	1906:1925	CONCLUSIONS During biofilm formation, the bacteria secreted surface biopolymers that differed from those of the planktonic cells.					
32216024	10	97	theme	P6-12	2011:2015	arg1	surface					2017:2023	the H. lusitanum P6-12 surface	1994:2023	the H. lusitanum P6-12 surface	1994:2023	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	10	98	theme	cell	2155:2158	arg1	adaptation					2160:2169	cell adaptation	2155:2169	cell adaptation to existence in plant tissues	2155:2199	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	7	99	dep	7	1579:1579	arg1	to					1576:1577	to	1576:1577	to	1576:1577	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	11	100	theme	better	2273:2278	arg1	understanding					2280:2292	a better understanding	2271:2292	a better understanding of the physiological properties of the biopolymers	2271:2343	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study permit a better understanding of the physiological properties of the biopolymers, for example, in plant-microbe interactions.					
32216024	10	101	theme	plant	2187:2191	arg1	tissues					2193:2199	plant tissues	2187:2199	plant tissues	2187:2199	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	11	102	theme	study	2258:2262	arg1	results					2243:2249	The results	2239:2249	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study	2202:2262	SIGNIFICANCE AND IMPACT OF THE STUDY The results of the study permit a better understanding of the physiological properties of the biopolymers, for example, in plant-microbe interactions.					
32216024	3	103	theme	polymer	647:653	arg1	separation					655:664	polymer separation	647:664	polymer separation	647:664	A high degree of polymer separation and purification was achieved by ultracentrifugation, and column chromatography allowed us to identify the chemical differences between biopolymers from biofilm and planktonic H. lusitanum.					
32216024	0	104	theme	planktonic	44:53	arg1	cells					67:71	planktonic and biofilm cells	44:71	planktonic and biofilm cells of Herbaspirillum lusitanum P6-12	44:105	Characterization of biopolymers produced by planktonic and biofilm cells of Herbaspirillum lusitanum P6-12.					
32216024	5	105	theme	EPS	1195:1197	arg1	CPS-II					1207:1212	The EPS, CPS-I, CPS-II	1191:1212	The EPS, CPS-I, CPS-II	1191:1212	The EPS, CPS-I, CPS-II had different monosaccharide and lipid compositions.					
32216024	10	106	gly	heterogeneity	1932:1944	arg1	biopolymers					1979:1989	the polysaccharide containing biopolymers	1949:1989	the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface	1949:2023	The heterogeneity of the polysaccharide containing biopolymers of the H. lusitanum P6-12 surface is probably conditioned by their different functions in plant colonization and formation of an efficient symbiosis, as well as in cell adaptation to existence in plant tissues.					
32216024	2	107	theme	strain	440:445	arg1	composition					378:388	biopolymers composition	366:388	biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12	366:451	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	2	108	theme	biopolymers	366:376	arg1	composition					378:388	biopolymers composition	366:388	biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12	366:451	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	0	109	theme	biofilm	59:65	arg1	cells					67:71	planktonic and biofilm cells	44:71	planktonic and biofilm cells of Herbaspirillum lusitanum P6-12	44:105	Characterization of biopolymers produced by planktonic and biofilm cells of Herbaspirillum lusitanum P6-12.					
32216024	1	110	theme	life	287:290	arg1	style					292:296	biofilm life style	279:296	biofilm life style	279:296	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	4	111	theme	high-molecular-weight	1008:1028	arg1	glycoconjugates					1030:1044	two high-molecular-weight glycoconjugates	1004:1044	two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS)	1004:1188	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	1	112	from	modes	177:181	arg1	sessile					322:328	sessile	322:328	sessile	322:328	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	1	112	from	modes	177:181	arg1	cells					312:316	the cells	308:316	the cells	308:316	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	1	112	from	modes	177:181	arg1	biopolymers					156:166	biopolymers	156:166	biopolymers	156:166	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32216024	2	113	dep	Fourier	473:479	arg1	transform					481:489	transform	481:489	transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry	481:627	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	2	114	from	Differences	351:361	arg1	cells					418:422	planktonic and biofilm cells	395:422	planktonic and biofilm cells	395:422	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	2	114	from	Differences	351:361	arg1	composition					378:388	biopolymers composition	366:388	biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12	366:451	METHODS AND RESULTS Differences in biopolymers composition from planktonic and biofilm cells of H. lusitanum strain P6-12 were analysed using Fourier transform infrared spectroscopy (FTIR), sodium dodecyl sulphate-polyacrylamide gel electrophoresis, gas-liquid chromatography and spectrophotometry.					
32216024	8	115	dep	10	1747:1748	arg1	to					1744:1745	to	1744:1745	to	1744:1745	The cells outside the biofilm had capsules containing high-molecular-weight glycoconjugate (was arbitrarily called CPSFBC ) that consisted of carbohydrates (up to 10%), proteins (up to 16%) and lipids (up to 70%).					
32216024	5	116	theme	different	1218:1226	arg1	monosaccharide					1228:1241	different monosaccharide and lipid compositions	1218:1264	monosaccharide	1228:1241	The EPS, CPS-I, CPS-II had different monosaccharide and lipid compositions.					
32216024	7	117	theme	polymeric	1467:1475	arg1	substance					1477:1485	a free extracellular polymeric substance	1446:1485	a free extracellular polymeric substance (was arbitrarily called fEPS)	1446:1515	From the biofilm culture medium, a free extracellular polymeric substance (was arbitrarily called fEPS) was obtained that contained proteins and carbohydrates (up to 7%).					
32216024	4	118	theme	lipopolysaccharide	1094:1111	arg1	nature					1119:1124	a lipopolysaccharide (LPS) nature	1092:1124	a lipopolysaccharide (LPS) nature	1092:1124	It was shown that planktonic cells of H. lusitanum P6-12 when cultivated in a liquid medium to the end of the exponential phase of growth, produced two high-molecular-weight glycoconjugates (were arbitrarily called CPS-I and CPS-II) of a lipopolysaccharide (LPS) nature and a lipid-polysacharide complex (were arbitrarily called EPS).					
32216024	1	119	dep	Herbaspirillum	190:203	arg1	lusitanum					205:213	lusitanum	205:213	lusitanum	205:213	AIMS The goal of this study was to characterize biopolymers from two modes of the Herbaspirillum lusitanum P6-12 growth: planktonic, in which cells are free swimming, and biofilm life style, in which the cells are sessile.					
32599232	1	0	with	co-precipitation	246:261	arg1	EPS					355:357	EPS	355:357	EPS	355:357	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	1	0	with	co-precipitation	246:261	arg1	exopolysaccharides					335:352	exopolysaccharides	335:352	exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris	335:404	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	3	1	theme	microscopy	501:510	arg1	results					518:524	Transmission electron microscopy (TEM) results	479:524	Transmission electron microscopy (TEM) results	479:524	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	3	2	theme	electron	492:499	arg1	microscopy					501:510	Transmission electron microscopy	479:510	Transmission electron microscopy (TEM) results	479:524	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	3	2	theme	electron	492:499	arg1	TEM					513:515	TEM	513:515	TEM	513:515	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	1	3	theme	chloride	277:284	arg1	co-precipitation					246:261	the co-precipitation	242:261	the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris	242:404	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	3	4	from	10-20 nm	631:638	arg1	size					643:646	size	643:646	size	643:646	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	7	5	theme	nanoparticles	1161:1173	arg1	composition					1115:1125	elemental composition	1105:1125	elemental composition	1105:1125	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	7	5	theme	nanoparticles	1161:1173	arg1	structure					1142:1150	electronic structure	1131:1150	electronic structure	1131:1150	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	1	6	theme	@	202:202	arg1	EPS					203:205	Fe3O4@EPS	197:205	Fe3O4@EPS	197:205	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	1	6	theme	@	202:202	arg1	particles					186:194	novel magnetic nano-composite particles	156:194	novel magnetic nano-composite particles (Fe3O4@EPS)	156:206	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	4	7	theme	Scanning	649:656	arg1	analysis					711:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	3	8	theme	Fe3O4	561:565	arg1	EPS					567:569	Fe3O4@EPS	561:569	Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size	561:646	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	6	9	theme	@	965:965	arg1	EPS					966:968	Fe3O4@EPS	960:968	Fe3O4@EPS	960:968	Fe3O4@EPS showed a highly magnetic nature at 5.0 emu/g.					
32599232	1	10	theme	microalga	377:385	arg1	vulgaris					397:404	the microalga Chlorella vulgaris	373:404	the microalga Chlorella vulgaris	373:404	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	8	11	theme	nutrient	1203:1210	arg1	removal					1212:1218	nutrient removal	1203:1218	nutrient removal from wastewater	1203:1234	Furthermore, nutrient removal from wastewater was studied.					
32599232	3	12	theme	EPS	567:569	arg1	nature					551:556	the core-shell nature	536:556	the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size	536:646	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	4	13	from	rate	815:818	arg1	ion-exchanger					796:808	the EPS polymeric ion-exchanger	778:808	the EPS polymeric ion-exchanger at a rate of 63.3% by weight	778:837	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	1	14	dep	chloride	277:284	arg1	III					272:274	III	272:274	III	272:274	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	7	15	theme	XPS	1020:1022	arg1	spectrum					1031:1038	The XPS survey spectrum	1016:1038	The XPS survey spectrum	1016:1038	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	3	16	theme	EPS	614:616	arg1	matrix					618:623	the indistinctly layered EPS matrix	589:623	the indistinctly layered EPS matrix to be 10-20 nm in size	589:646	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	5	17	theme	functional	926:935	arg1	groups					937:942	the functional groups	922:942	the functional groups present in EPS	922:957	FT-IR results demonstrated that Fe3O4 nanoparticles were successfully modified by the functional groups present in EPS.					
32599232	4	18	theme	microscopy-based	667:682	arg1	analysis					711:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	0	19	theme	vulgaris	65:72	arg1	exopolysaccharides					74:91	Chlorella vulgaris exopolysaccharides	55:91	Chlorella vulgaris exopolysaccharides immobilized in iron-magnetic nanoparticles	55:134	Non-toxic nano approach for wastewater treatment using Chlorella vulgaris exopolysaccharides immobilized in iron-magnetic nanoparticles.					
32599232	1	20	theme	nano-composite	171:184	arg1	EPS					203:205	Fe3O4@EPS	197:205	Fe3O4@EPS	197:205	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	1	20	theme	nano-composite	171:184	arg1	particles					186:194	novel magnetic nano-composite particles	156:194	novel magnetic nano-composite particles (Fe3O4@EPS)	156:206	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	4	21	theme	dispersive	691:700	arg1	analysis					711:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	0	22	theme	iron-magnetic	108:120	arg1	nanoparticles					122:134	iron-magnetic nanoparticles	108:134	iron-magnetic nanoparticles	108:134	Non-toxic nano approach for wastewater treatment using Chlorella vulgaris exopolysaccharides immobilized in iron-magnetic nanoparticles.					
32599232	9	23	theme	Fe3O4	1286:1290	arg1	EPS					1292:1294	Fe3O4@EPS	1286:1294	Fe3O4@EPS	1286:1294	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	1	24	theme	novel	156:160	arg1	EPS					203:205	Fe3O4@EPS	197:205	Fe3O4@EPS	197:205	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	1	24	theme	novel	156:160	arg1	particles					186:194	novel magnetic nano-composite particles	156:194	novel magnetic nano-composite particles (Fe3O4@EPS)	156:206	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	7	25	theme	Fe3O4	1179:1183	arg1	EPS					1185:1187	Fe3O4@EPS	1179:1187	Fe3O4@EPS	1179:1187	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	9	26	theme	EPS	1292:1294	arg1	3.5 g/L					1275:1281	3.5 g/L	1275:1281	3.5 g/L of Fe3O4@EPS	1275:1294	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	26	theme	EPS	1292:1294	arg1	13 h					1308:1311	13 h	1308:1311	13 h	1308:1311	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	26	theme	EPS	1292:1294	arg1	pH 7.0					1297:1302	pH 7.0	1297:1302	pH 7.0	1297:1302	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	26	theme	EPS	1292:1294	arg1	incubation					1316:1325	incubation	1316:1325	incubation	1316:1325	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	2	27	theme	Fe3O4	442:446	arg1	EPS					448:450	the Fe3O4@EPS	438:450	the Fe3O4@EPS	438:450	The physico-chemical nature of the Fe3O4@EPS was investigated in depth.					
32599232	1	28	dep	sulfate	300:306	arg1	II					296:297	II	296:297	II	296:297	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	0	29	theme	Non-toxic	0:8	arg1	approach					15:22	Non-toxic nano approach	0:22	Non-toxic nano approach for wastewater treatment	0:47	Non-toxic nano approach for wastewater treatment using Chlorella vulgaris exopolysaccharides immobilized in iron-magnetic nanoparticles.					
32599232	5	30	theme	present	944:950	arg1	groups					937:942	the functional groups	922:942	the functional groups present in EPS	922:957	FT-IR results demonstrated that Fe3O4 nanoparticles were successfully modified by the functional groups present in EPS.					
32599232	2	31	theme	physico-chemical	411:426	arg1	nature					428:433	The physico-chemical nature	407:433	The physico-chemical nature of the Fe3O4@EPS	407:450	The physico-chemical nature of the Fe3O4@EPS was investigated in depth.					
32599232	1	32	theme	magnetic	162:169	arg1	EPS					203:205	Fe3O4@EPS	197:205	Fe3O4@EPS	197:205	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	1	32	theme	magnetic	162:169	arg1	particles					186:194	novel magnetic nano-composite particles	156:194	novel magnetic nano-composite particles (Fe3O4@EPS)	156:206	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	7	33	from	724.1	1073:1077	arg1	peaks					1064:1068	two major peaks	1054:1068	two major peaks at 724.1 and 710.2 eV	1054:1090	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	10	34	theme	@	1434:1434	arg1	EPS					1435:1437	Fe3O4@EPS	1429:1437	Fe3O4@EPS	1429:1437	These findings demonstrate the potential of Fe3O4@EPS for removing PO43- and NH4+ in wastewater treatment plants.					
32599232	5	35	theme	FT-IR	840:844	arg1	results					846:852	FT-IR results	840:852	FT-IR results	840:852	FT-IR results demonstrated that Fe3O4 nanoparticles were successfully modified by the functional groups present in EPS.					
32599232	9	36	theme	incubation	1316:1325	arg1	3.5 g/L					1275:1281	3.5 g/L	1275:1281	3.5 g/L of Fe3O4@EPS	1275:1294	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	36	theme	incubation	1316:1325	arg1	pH 7.0					1297:1302	pH 7.0	1297:1302	pH 7.0	1297:1302	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	36	theme	incubation	1316:1325	arg1	incubation					1316:1325	incubation	1316:1325	incubation	1316:1325	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	0	37	theme	wastewater	28:37	arg1	treatment					39:47	wastewater treatment	28:47	wastewater treatment	28:47	Non-toxic nano approach for wastewater treatment using Chlorella vulgaris exopolysaccharides immobilized in iron-magnetic nanoparticles.					
32599232	2	38	theme	EPS	448:450	arg1	nature					428:433	The physico-chemical nature	407:433	The physico-chemical nature of the Fe3O4@EPS	407:450	The physico-chemical nature of the Fe3O4@EPS was investigated in depth.					
32599232	7	39	from	710.2 eV	1083:1090	arg1	peaks					1064:1068	two major peaks	1054:1068	two major peaks at 724.1 and 710.2 eV	1054:1090	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	4	40	theme	polymeric	786:794	arg1	ion-exchanger					796:808	the EPS polymeric ion-exchanger	778:808	the EPS polymeric ion-exchanger at a rate of 63.3% by weight	778:837	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	5	41	from	EPS	955:957	arg1	present					944:950	present	944:950	present	944:950	FT-IR results demonstrated that Fe3O4 nanoparticles were successfully modified by the functional groups present in EPS.					
32599232	5	42	mod	modified	910:917	arg3	groups					937:942	the functional groups	922:942	the functional groups present in EPS	922:957	FT-IR results demonstrated that Fe3O4 nanoparticles were successfully modified by the functional groups present in EPS.					
32599232	5	42	mod	modified	910:917	arg1	nanoparticles					878:890	Fe3O4 nanoparticles	872:890	Fe3O4 nanoparticles	872:890	FT-IR results demonstrated that Fe3O4 nanoparticles were successfully modified by the functional groups present in EPS.					
32599232	5	43	from	present	944:950	arg1	EPS					955:957	EPS	955:957	EPS	955:957	FT-IR results demonstrated that Fe3O4 nanoparticles were successfully modified by the functional groups present in EPS.					
32599232	0	44	theme	Chlorella	55:63	arg1	exopolysaccharides					74:91	Chlorella vulgaris exopolysaccharides	55:91	Chlorella vulgaris exopolysaccharides immobilized in iron-magnetic nanoparticles	55:134	Non-toxic nano approach for wastewater treatment using Chlorella vulgaris exopolysaccharides immobilized in iron-magnetic nanoparticles.					
32599232	10	45	theme	wastewater	1470:1479	arg1	plants					1491:1496	wastewater treatment plants	1470:1496	wastewater treatment plants	1470:1496	These findings demonstrate the potential of Fe3O4@EPS for removing PO43- and NH4+ in wastewater treatment plants.					
32599232	1	46	attach	derived	360:366	arg2	EPS					355:357	EPS	355:357	EPS	355:357	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	1	46	attach	derived	360:366	arg2	exopolysaccharides					335:352	exopolysaccharides	335:352	exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris	335:404	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	1	46	attach	derived	360:366	arg1	vulgaris					397:404	the microalga Chlorella vulgaris	373:404	the microalga Chlorella vulgaris	373:404	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	9	47	theme	NH4+were	1352:1359	arg1	NH4+were					1352:1359	NH4+were	1352:1359	NH4+were	1352:1359	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	47	theme	NH4+were	1352:1359	arg1	PO43-					1335:1339	PO43-	1335:1339	PO43-	1335:1339	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	47	theme	NH4+were	1352:1359	arg1	%					1347:1347	85%	1345:1347	85% of NH4+were	1345:1359	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	47	theme	NH4+were	1352:1359	arg1	%					1330:1330	91%	1328:1330	91% of PO43-	1328:1339	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	1	48	theme	Fe3O4	197:201	arg1	EPS					203:205	Fe3O4@EPS	197:205	Fe3O4@EPS	197:205	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	1	48	theme	Fe3O4	197:201	arg1	particles					186:194	novel magnetic nano-composite particles	156:194	novel magnetic nano-composite particles (Fe3O4@EPS)	156:206	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	4	49	theme	%	827:827	arg1	rate					815:818	a rate	813:818	a rate of 63.3% by weight	813:837	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	7	50	theme	major	1058:1062	arg1	peaks					1064:1068	two major peaks	1054:1068	two major peaks at 724.1 and 710.2 eV	1054:1090	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	7	51	theme	electronic	1131:1140	arg1	structure					1142:1150	electronic structure	1131:1150	electronic structure	1131:1150	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	3	52	theme	core-shell	540:549	arg1	nature					551:556	the core-shell nature	536:556	the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size	536:646	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	6	53	theme	Fe3O4	960:964	arg1	EPS					966:968	Fe3O4@EPS	960:968	Fe3O4@EPS	960:968	Fe3O4@EPS showed a highly magnetic nature at 5.0 emu/g.					
32599232	10	54	from	PO43-	1452:1456	arg1	plants					1491:1496	wastewater treatment plants	1470:1496	wastewater treatment plants	1470:1496	These findings demonstrate the potential of Fe3O4@EPS for removing PO43- and NH4+ in wastewater treatment plants.					
32599232	5	55	attach	present	944:950	arg2	groups					937:942	the functional groups	922:942	the functional groups present in EPS	922:957	FT-IR results demonstrated that Fe3O4 nanoparticles were successfully modified by the functional groups present in EPS.					
32599232	5	55	attach	present	944:950	arg1	EPS					955:957	EPS	955:957	EPS	955:957	FT-IR results demonstrated that Fe3O4 nanoparticles were successfully modified by the functional groups present in EPS.					
32599232	7	56	theme	survey	1024:1029	arg1	spectrum					1031:1038	The XPS survey spectrum	1016:1038	The XPS survey spectrum	1016:1038	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	1	57	theme	Chlorella	387:395	arg1	vulgaris					397:404	the microalga Chlorella vulgaris	373:404	the microalga Chlorella vulgaris	373:404	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	3	58	theme	@	566:566	arg1	EPS					567:569	Fe3O4@EPS	561:569	Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size	561:646	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	8	59	from	wastewater	1225:1234	arg1	removal					1212:1218	nutrient removal	1203:1218	nutrient removal from wastewater	1203:1234	Furthermore, nutrient removal from wastewater was studied.					
32599232	1	60	theme	current	141:147	arg1	study					149:153	The current study	137:153	The current study	137:153	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	4	61	theme	spectral	702:709	arg1	analysis					711:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	3	62	theme	layered	606:612	arg1	matrix					618:623	the indistinctly layered EPS matrix	589:623	the indistinctly layered EPS matrix to be 10-20 nm in size	589:646	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	9	63	theme	optimum	1255:1261	arg1	conditions					1263:1272	optimum conditions	1255:1272	optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation)	1255:1326	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	5	64	theme	Fe3O4	872:876	arg1	nanoparticles					878:890	Fe3O4 nanoparticles	872:890	Fe3O4 nanoparticles	872:890	FT-IR results demonstrated that Fe3O4 nanoparticles were successfully modified by the functional groups present in EPS.					
32599232	4	65	theme	electron	658:665	arg1	analysis					711:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	7	66	theme	EPS	1185:1187	arg1	composition					1115:1125	elemental composition	1105:1125	elemental composition	1105:1125	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	7	66	theme	EPS	1185:1187	arg1	structure					1142:1150	electronic structure	1131:1150	electronic structure	1131:1150	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	6	67	theme	magnetic	986:993	arg1	nature					995:1000	a highly magnetic nature	977:1000	a highly magnetic nature at 5.0 emu/g	977:1013	Fe3O4@EPS showed a highly magnetic nature at 5.0 emu/g.					
32599232	4	68	theme	energy	684:689	arg1	analysis					711:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis	649:718	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	7	69	theme	@	1184:1184	arg1	EPS					1185:1187	Fe3O4@EPS	1179:1187	Fe3O4@EPS	1179:1187	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	10	70	from	NH4+	1462:1465	arg1	plants					1491:1496	wastewater treatment plants	1470:1496	wastewater treatment plants	1470:1496	These findings demonstrate the potential of Fe3O4@EPS for removing PO43- and NH4+ in wastewater treatment plants.					
32599232	6	71	from	5.0 emu/g	1005:1013	arg1	nature					995:1000	a highly magnetic nature	977:1000	a highly magnetic nature at 5.0 emu/g	977:1013	Fe3O4@EPS showed a highly magnetic nature at 5.0 emu/g.					
32599232	9	72	theme	@	1291:1291	arg1	EPS					1292:1294	Fe3O4@EPS	1286:1294	Fe3O4@EPS	1286:1294	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	7	73	dep	composition	1115:1125	arg1	the					1101:1103	the	1101:1103	the	1101:1103	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	0	74	theme	nano	10:13	arg1	approach					15:22	Non-toxic nano approach	0:22	Non-toxic nano approach for wastewater treatment	0:47	Non-toxic nano approach for wastewater treatment using Chlorella vulgaris exopolysaccharides immobilized in iron-magnetic nanoparticles.					
32599232	4	75	theme	elemental	735:743	arg1	Fe					745:746	elemental Fe	735:746	elemental Fe	735:746	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	1	76	theme	iron	290:293	arg1	sulfate					300:306	iron (II) sulfate	290:306	iron (II) sulfate	290:306	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	9	77	dep	conditions	1263:1272	arg1	3.5 g/L					1275:1281	3.5 g/L	1275:1281	3.5 g/L of Fe3O4@EPS	1275:1294	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	77	dep	conditions	1263:1272	arg1	13 h					1308:1311	13 h	1308:1311	13 h	1308:1311	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	77	dep	conditions	1263:1272	arg1	pH 7.0					1297:1302	pH 7.0	1297:1302	pH 7.0	1297:1302	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	77	dep	conditions	1263:1272	arg1	incubation					1316:1325	incubation	1316:1325	incubation	1316:1325	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	10	78	theme	EPS	1435:1437	arg1	potential					1416:1424	the potential	1412:1424	the potential of Fe3O4@EPS for removing PO43- and NH4+ in wastewater treatment plants	1412:1496	These findings demonstrate the potential of Fe3O4@EPS for removing PO43- and NH4+ in wastewater treatment plants.					
32599232	10	79	theme	Fe3O4	1429:1433	arg1	EPS					1435:1437	Fe3O4@EPS	1429:1437	Fe3O4@EPS	1429:1437	These findings demonstrate the potential of Fe3O4@EPS for removing PO43- and NH4+ in wastewater treatment plants.					
32599232	7	80	theme	elemental	1105:1113	arg1	composition					1115:1125	elemental composition	1105:1125	elemental composition	1105:1125	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	1	81	theme	sulfate	300:306	arg1	co-precipitation					246:261	the co-precipitation	242:261	the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris	242:404	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	10	82	theme	treatment	1481:1489	arg1	plants					1491:1496	wastewater treatment plants	1470:1496	wastewater treatment plants	1470:1496	These findings demonstrate the potential of Fe3O4@EPS for removing PO43- and NH4+ in wastewater treatment plants.					
32599232	2	83	theme	@	447:447	arg1	EPS					448:450	the Fe3O4@EPS	438:450	the Fe3O4@EPS	438:450	The physico-chemical nature of the Fe3O4@EPS was investigated in depth.					
32599232	3	84	theme	Transmission	479:490	arg1	microscopy					501:510	Transmission electron microscopy	479:510	Transmission electron microscopy (TEM) results	479:524	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	3	84	theme	Transmission	479:490	arg1	TEM					513:515	TEM	513:515	TEM	513:515	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	1	85	theme	Fe3O4	309:313	arg1	nanoparticles					315:327	Fe3O4 nanoparticles	309:327	Fe3O4 nanoparticles	309:327	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	1	85	theme	Fe3O4	309:313	arg1	chloride					277:284	iron (III) chloride	266:284	iron (III) chloride	266:284	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	4	86	theme	EPS	782:784	arg1	ion-exchanger					796:808	the EPS polymeric ion-exchanger	778:808	the EPS polymeric ion-exchanger at a rate of 63.3% by weight	778:837	Scanning electron microscopy-based energy dispersive spectral analysis indicated that elemental Fe was successfully loaded on to the EPS polymeric ion-exchanger at a rate of 63.3% by weight.					
32599232	9	87	theme	PO43-	1335:1339	arg1	NH4+were					1352:1359	NH4+were	1352:1359	NH4+were	1352:1359	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	87	theme	PO43-	1335:1339	arg1	PO43-					1335:1339	PO43-	1335:1339	PO43-	1335:1339	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	87	theme	PO43-	1335:1339	arg1	%					1347:1347	85%	1345:1347	85% of NH4+were	1345:1359	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	9	87	theme	PO43-	1335:1339	arg1	%					1330:1330	91%	1328:1330	91% of PO43-	1328:1339	Under optimum conditions (3.5 g/L of Fe3O4@EPS, pH 7.0 and 13 h of incubation) 91% of PO43- and 85% of NH4+were effectively eliminated.					
32599232	3	88	from	size	643:646	arg1	10-20 nm					631:638	10-20 nm	631:638	10-20 nm	631:638	Transmission electron microscopy (TEM) results estimated the core-shell nature of Fe3O4@EPS aggregated inside the indistinctly layered EPS matrix to be 10-20 nm in size.					
32599232	7	89	theme	Fe3O4	1155:1159	arg1	nanoparticles					1161:1173	Fe3O4 nanoparticles	1155:1173	Fe3O4 nanoparticles	1155:1173	The XPS survey spectrum, which showed two major peaks at 724.1 and 710.2 eV revealed the elemental composition and electronic structure of Fe3O4 nanoparticles and Fe3O4@EPS.					
32599232	1	90	theme	iron	266:269	arg1	nanoparticles					315:327	Fe3O4 nanoparticles	309:327	Fe3O4 nanoparticles	309:327	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
32599232	1	90	theme	iron	266:269	arg1	chloride					277:284	iron (III) chloride	266:284	iron (III) chloride	266:284	The current study, novel magnetic nano-composite particles (Fe3O4@EPS) were successfully synthesized via the co-precipitation of iron (III) chloride and iron (II) sulfate (Fe3O4 nanoparticles) with exopolysaccharides (EPS) derived from the microalga Chlorella vulgaris.					
33938215	8	0	theme	hybrid	1020:1025	arg1	gel					1037:1039	the hybrid composite gel	1016:1039	the hybrid composite gel	1016:1039	Further, in vivo study demonstrates that the hybrid composite gel can be used as a therapeutic for pressure-induced ulcers.					
33938215	8	0	theme	hybrid	1020:1025	arg1	therapeutic					1058:1068	therapeutic	1058:1068	therapeutic	1058:1068	Further, in vivo study demonstrates that the hybrid composite gel can be used as a therapeutic for pressure-induced ulcers.					
33938215	1	1	theme	mechanical	177:186	arg1	properties					188:197	the mechanical properties	173:197	the mechanical properties of the resulting hydrogels	173:224	The addition of particles during the sol-to-gel conversion process generally enhances the mechanical properties of the resulting hydrogels.					
33938215	7	2	theme	60	914:915	arg1	%					916:916	%	916:916	%	916:916	The resulting hybrid gel reveals reversible compressibility up to a 60% strain and high stretchability even up to ∼400% in area.					
33938215	8	3	used	used	1048:1051	arg2	gel					1037:1039	the hybrid composite gel	1016:1039	the hybrid composite gel	1016:1039	Further, in vivo study demonstrates that the hybrid composite gel can be used as a therapeutic for pressure-induced ulcers.					
33938215	8	3	used	used	1048:1051	arg2	therapeutic					1058:1068	therapeutic	1058:1068	therapeutic	1058:1068	Further, in vivo study demonstrates that the hybrid composite gel can be used as a therapeutic for pressure-induced ulcers.					
33938215	7	4	dep	strain	918:923	arg1	up					906:907	up	906:907	up	906:907	The resulting hybrid gel reveals reversible compressibility up to a 60% strain and high stretchability even up to ∼400% in area.					
33938215	3	5	theme	natural	386:392	arg1	particles					401:409	natural porous particles	386:409	natural porous particles called diatom frustule silica	386:439	Herein, we report hydrogel-to-elastomer conversions by natural porous particles called diatom frustule silica, namely, Melosira nummuloides.					
33938215	4	6	theme	interlocking	509:520	arg1	points					522:527	mechanical interlocking points	498:527	mechanical interlocking points for polymers that are reinforced by gelation	498:572	The surface pores provide mechanical interlocking points for polymers that are reinforced by gelation.					
33938215	8	7	theme	composite	1027:1035	arg1	gel					1037:1039	the hybrid composite gel	1016:1039	the hybrid composite gel	1016:1039	Further, in vivo study demonstrates that the hybrid composite gel can be used as a therapeutic for pressure-induced ulcers.					
33938215	8	7	theme	composite	1027:1035	arg1	therapeutic					1058:1068	therapeutic	1058:1068	therapeutic	1058:1068	Further, in vivo study demonstrates that the hybrid composite gel can be used as a therapeutic for pressure-induced ulcers.					
33938215	8	8	theme	in	984:985	arg1	study					992:996	in vivo study	984:996	in vivo study	984:996	Further, in vivo study demonstrates that the hybrid composite gel can be used as a therapeutic for pressure-induced ulcers.					
33938215	7	9	theme	reversible	879:888	arg1	compressibility					890:904	reversible compressibility	879:904	reversible compressibility up to a 60% strain and high stretchability	879:947	The resulting hybrid gel reveals reversible compressibility up to a 60% strain and high stretchability even up to ∼400% in area.					
33938215	3	10	theme	hydrogel-to-elastomer	349:369	arg1	nummuloides					459:469	Melosira nummuloides	450:469	Melosira nummuloides	450:469	Herein, we report hydrogel-to-elastomer conversions by natural porous particles called diatom frustule silica, namely, Melosira nummuloides.					
33938215	3	10	theme	hydrogel-to-elastomer	349:369	arg1	conversions					371:381	hydrogel-to-elastomer conversions	349:381	hydrogel-to-elastomer conversions	349:381	Herein, we report hydrogel-to-elastomer conversions by natural porous particles called diatom frustule silica, namely, Melosira nummuloides.					
33938215	4	11	theme	mechanical	498:507	arg1	points					522:527	mechanical interlocking points	498:527	mechanical interlocking points for polymers that are reinforced by gelation	498:572	The surface pores provide mechanical interlocking points for polymers that are reinforced by gelation.					
33938215	5	12	theme	adhesion	669:676	arg1	catechol					696:703	catechol	696:703	catechol	696:703	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	5	12	theme	adhesion	669:676	arg1	moieties					678:685	water-resistant adhesion moieties	653:685	water-resistant adhesion moieties	653:685	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	5	13	theme	water-resistant	653:667	arg1	catechol					696:703	catechol	696:703	catechol	696:703	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	5	13	theme	water-resistant	653:667	arg1	moieties					678:685	water-resistant adhesion moieties	653:685	water-resistant adhesion moieties	653:685	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	5	14	theme	moieties	678:685	arg1	presence					641:648	the presence	637:648	the presence of water-resistant adhesion moieties, such as catechol	637:703	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	5	14	theme	moieties	678:685	arg1	aspect					593:598	The most critical aspect	575:598	The most critical aspect when choosing polymeric materials	575:632	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	0	15	theme	Diatom	0:5	arg1	Adhesion					52:59	Adhesion	52:59	Adhesion	52:59	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	0	15	theme	Diatom	0:5	arg1	Synergy					78:84	Interlocking Synergy	65:84	Interlocking Synergy	65:84	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	0	15	theme	Diatom	0:5	arg1	Hydrogels					41:49	Diatom Silica/Polysaccharide Elastomeric Hydrogels	0:49	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.	0:85	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	0	16	theme	Silica/Polysaccharide	7:27	arg1	Adhesion					52:59	Adhesion	52:59	Adhesion	52:59	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	0	16	theme	Silica/Polysaccharide	7:27	arg1	Synergy					78:84	Interlocking Synergy	65:84	Interlocking Synergy	65:84	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	0	16	theme	Silica/Polysaccharide	7:27	arg1	Hydrogels					41:49	Diatom Silica/Polysaccharide Elastomeric Hydrogels	0:49	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.	0:85	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	5	17	theme	polymeric	614:622	arg1	materials					624:632	polymeric materials	614:632	polymeric materials	614:632	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	1	18	theme	resulting	206:214	arg1	hydrogels					216:224	the resulting hydrogels	202:224	the resulting hydrogels	202:224	The addition of particles during the sol-to-gel conversion process generally enhances the mechanical properties of the resulting hydrogels.					
33938215	3	19	theme	diatom	418:423	arg1	silica					434:439	diatom frustule silica	418:439	diatom frustule silica	418:439	Herein, we report hydrogel-to-elastomer conversions by natural porous particles called diatom frustule silica, namely, Melosira nummuloides.					
33938215	1	20	theme	particles	103:111	arg1	addition					91:98	The addition	87:98	The addition of particles during the sol-to-gel conversion process	87:152	The addition of particles during the sol-to-gel conversion process generally enhances the mechanical properties of the resulting hydrogels.					
33938215	7	21	theme	resulting	850:858	arg1	gel					867:869	The resulting hybrid gel	846:869	The resulting hybrid gel	846:869	The resulting hybrid gel reveals reversible compressibility up to a 60% strain and high stretchability even up to ∼400% in area.					
33938215	3	22	theme	Melosira	450:457	arg1	nummuloides					459:469	Melosira nummuloides	450:469	Melosira nummuloides	450:469	Herein, we report hydrogel-to-elastomer conversions by natural porous particles called diatom frustule silica, namely, Melosira nummuloides.					
33938215	3	22	theme	Melosira	450:457	arg1	conversions					371:381	hydrogel-to-elastomer conversions	349:381	hydrogel-to-elastomer conversions	349:381	Herein, we report hydrogel-to-elastomer conversions by natural porous particles called diatom frustule silica, namely, Melosira nummuloides.					
33938215	3	23	theme	porous	394:399	arg1	particles					401:409	natural porous particles	386:409	natural porous particles called diatom frustule silica	386:439	Herein, we report hydrogel-to-elastomer conversions by natural porous particles called diatom frustule silica, namely, Melosira nummuloides.					
33938215	6	24	theme	elastomeric	812:822	arg1	hydrogel					824:831	no elastomeric hydrogel	809:831	no elastomeric hydrogel	809:831	Without catechol, no sol-to-gel conversion is observed; thus, no elastomeric hydrogel is produced.					
33938215	2	25	theme	open	316:319	arg1	question					321:328	an open question	313:328	an open question	313:328	However, the impact of the addition of porous particles during such a process remains an open question.					
33938215	7	26	theme	hybrid	860:865	arg1	gel					867:869	The resulting hybrid gel	846:869	The resulting hybrid gel	846:869	The resulting hybrid gel reveals reversible compressibility up to a 60% strain and high stretchability even up to ∼400% in area.					
33938215	0	27	theme	Elastomeric	29:39	arg1	Adhesion					52:59	Adhesion	52:59	Adhesion	52:59	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	0	27	theme	Elastomeric	29:39	arg1	Synergy					78:84	Interlocking Synergy	65:84	Interlocking Synergy	65:84	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	0	27	theme	Elastomeric	29:39	arg1	Hydrogels					41:49	Diatom Silica/Polysaccharide Elastomeric Hydrogels	0:49	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.	0:85	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	4	28	theme	surface	476:482	arg1	pores					484:488	The surface pores	472:488	The surface pores	472:488	The surface pores provide mechanical interlocking points for polymers that are reinforced by gelation.					
33938215	8	29	dep	in	984:985	arg1	vivo					987:990	vivo	987:990	vivo	987:990	Further, in vivo study demonstrates that the hybrid composite gel can be used as a therapeutic for pressure-induced ulcers.					
33938215	8	30	theme	pressure-induced	1074:1089	arg1	ulcers					1091:1096	pressure-induced ulcers	1074:1096	pressure-induced ulcers	1074:1096	Further, in vivo study demonstrates that the hybrid composite gel can be used as a therapeutic for pressure-induced ulcers.					
33938215	2	31	theme	addition	254:261	arg1	impact					240:245	the impact	236:245	the impact of the addition of porous particles during such a process	236:303	However, the impact of the addition of porous particles during such a process remains an open question.					
33938215	1	32	theme	hydrogels	216:224	arg1	properties					188:197	the mechanical properties	173:197	the mechanical properties of the resulting hydrogels	173:224	The addition of particles during the sol-to-gel conversion process generally enhances the mechanical properties of the resulting hydrogels.					
33938215	9	33	theme	100	1217:1219	arg1	%					1220:1220	%	1220:1220	%	1220:1220	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33938215	5	34	theme	most	579:582	arg1	presence					641:648	the presence	637:648	the presence of water-resistant adhesion moieties, such as catechol	637:703	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	5	34	theme	most	579:582	arg1	aspect					593:598	The most critical aspect	575:598	The most critical aspect when choosing polymeric materials	575:632	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	3	35	theme	frustule	425:432	arg1	silica					434:439	diatom frustule silica	418:439	diatom frustule silica	418:439	Herein, we report hydrogel-to-elastomer conversions by natural porous particles called diatom frustule silica, namely, Melosira nummuloides.					
33938215	9	36	theme	%	1220:1220	arg1	materials					1246:1254	100% water-based elastomeric materials	1217:1254	100% water-based elastomeric materials	1217:1254	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33938215	5	37	theme	critical	584:591	arg1	presence					641:648	the presence	637:648	the presence of water-resistant adhesion moieties, such as catechol	637:703	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	5	37	theme	critical	584:591	arg1	aspect					593:598	The most critical aspect	575:598	The most critical aspect when choosing polymeric materials	575:632	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	1	38	theme	sol-to-gel	124:133	arg1	process					146:152	the sol-to-gel conversion process	120:152	the sol-to-gel conversion process	120:152	The addition of particles during the sol-to-gel conversion process generally enhances the mechanical properties of the resulting hydrogels.					
33938215	5	39	theme	polymer	714:720	arg1	chain					722:726	a polymer chain	712:726	a polymer chain	712:726	The most critical aspect when choosing polymeric materials is the presence of water-resistant adhesion moieties, such as catechol, along a polymer chain, such as chitosan.					
33938215	9	40	theme	water-based	1222:1232	arg1	materials					1246:1254	100% water-based elastomeric materials	1217:1254	100% water-based elastomeric materials	1217:1254	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33938215	9	41	theme	adhesion	1123:1130	arg1	synergy					1103:1109	The synergy	1099:1109	The synergy of chemical adhesion and physical chain entanglement via pores	1099:1172	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33938215	1	42	theme	conversion	135:144	arg1	process					146:152	the sol-to-gel conversion process	120:152	the sol-to-gel conversion process	120:152	The addition of particles during the sol-to-gel conversion process generally enhances the mechanical properties of the resulting hydrogels.					
33938215	7	43	dep	∼400	960:963	arg1	to					957:958	to	957:958	to	957:958	The resulting hybrid gel reveals reversible compressibility up to a 60% strain and high stretchability even up to ∼400% in area.					
33938215	9	44	theme	new	1204:1206	arg1	class					1208:1212	a new class	1202:1212	a new class of 100% water-based elastomeric materials	1202:1254	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33938215	9	45	theme	elastomeric	1234:1244	arg1	materials					1246:1254	100% water-based elastomeric materials	1217:1254	100% water-based elastomeric materials	1217:1254	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33938215	0	46	dep	Hydrogels	41:49	arg1	Adhesion					52:59	Adhesion	52:59	Adhesion	52:59	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	0	46	dep	Hydrogels	41:49	arg1	Synergy					78:84	Interlocking Synergy	65:84	Interlocking Synergy	65:84	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	0	46	dep	Hydrogels	41:49	arg1	Hydrogels					41:49	Diatom Silica/Polysaccharide Elastomeric Hydrogels	0:49	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.	0:85	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	6	47	theme	sol-to-gel	768:777	arg1	conversion					779:788	no sol-to-gel conversion	765:788	no sol-to-gel conversion	765:788	Without catechol, no sol-to-gel conversion is observed; thus, no elastomeric hydrogel is produced.					
33938215	7	48	theme	high	929:932	arg1	stretchability					934:947	high stretchability	929:947	high stretchability	929:947	The resulting hybrid gel reveals reversible compressibility up to a 60% strain and high stretchability even up to ∼400% in area.					
33938215	9	49	theme	chemical	1114:1121	arg1	adhesion					1123:1130	chemical adhesion	1114:1130	chemical adhesion	1114:1130	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33938215	9	50	theme	materials	1246:1254	arg1	class					1208:1212	a new class	1202:1212	a new class of 100% water-based elastomeric materials	1202:1254	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33938215	2	51	theme	particles	273:281	arg1	addition					254:261	the addition	250:261	the addition of porous particles	250:281	However, the impact of the addition of porous particles during such a process remains an open question.					
33938215	9	52	theme	physical	1136:1143	arg1	chain					1145:1149	physical chain entanglement	1136:1162	physical chain entanglement	1136:1162	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33938215	7	53	theme	%	916:916	arg1	strain					918:923	a 60% strain	912:923	a 60% strain	912:923	The resulting hybrid gel reveals reversible compressibility up to a 60% strain and high stretchability even up to ∼400% in area.					
33938215	2	54	theme	porous	266:271	arg1	particles					273:281	porous particles	266:281	porous particles	266:281	However, the impact of the addition of porous particles during such a process remains an open question.					
33938215	9	55	theme	chain	1145:1149	arg1	synergy					1103:1109	The synergy	1099:1109	The synergy of chemical adhesion and physical chain entanglement via pores	1099:1172	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33938215	7	56	dep	%	964:964	arg1	area					969:972	area	969:972	area	969:972	The resulting hybrid gel reveals reversible compressibility up to a 60% strain and high stretchability even up to ∼400% in area.					
33938215	0	57	theme	Interlocking	65:76	arg1	Synergy					78:84	Interlocking Synergy	65:84	Interlocking Synergy	65:84	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	0	57	theme	Interlocking	65:76	arg1	Hydrogels					41:49	Diatom Silica/Polysaccharide Elastomeric Hydrogels	0:49	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.	0:85	Diatom Silica/Polysaccharide Elastomeric Hydrogels: Adhesion and Interlocking Synergy.					
33938215	9	58	theme	entanglement	1151:1162	arg1	chain					1145:1149	physical chain entanglement	1136:1162	physical chain entanglement	1136:1162	The synergy of chemical adhesion and physical chain entanglement via pores provides a way to fabricate a new class of 100% water-based elastomeric materials.					
33964661	4	0	theme	rye	673:675	arg1	flours					688:693	PA-treated rye and barley flours	662:693	PA-treated rye and barley flours	662:693	Interestingly, viscosity peaked distinctively ~75 °C in PA-treated rye and barley flours, and their isolated protein-starch systems, indicating prolamins unravelled and complexed with PA during heating.					
33964661	0	1	with	interactions	27:38	arg1	polysaccharides					75:89	non-starch polysaccharides	64:89	non-starch polysaccharides	64:89	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.					
33964661	0	1	with	interactions	27:38	arg1	proteins					51:58	grain proteins	45:58	grain proteins	45:58	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.					
33964661	1	2	theme	gluten	160:165	arg1	proteins					167:174	wheat gluten proteins	154:174	wheat gluten proteins	154:174	Proanthocyanidins (PA) cross-link wheat gluten proteins and dramatically enhance batter viscosity; PA could similarly affect related grains.					
33964661	7	3	from	effects	1130:1136	arg1	NSP					1164:1166	NSP	1164:1166	NSP	1164:1166	Thus, rye flour viscosity dramatically increased through interactive effects of PA on rye proteins and NSP, which could expand its food applications.					
33964661	7	3	from	effects	1130:1136	arg1	proteins					1151:1158	rye proteins	1147:1158	rye proteins	1147:1158	Thus, rye flour viscosity dramatically increased through interactive effects of PA on rye proteins and NSP, which could expand its food applications.					
33964661	7	4	theme	flour	1071:1075	arg1	viscosity					1077:1085	rye flour viscosity	1067:1085	rye flour viscosity	1067:1085	Thus, rye flour viscosity dramatically increased through interactive effects of PA on rye proteins and NSP, which could expand its food applications.					
33964661	2	5	theme	rye	348:350	arg1	viscosity					304:312	viscosity	304:312	viscosity	304:312	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	5	theme	rye	348:350	arg1	properties					326:335	pasting properties	318:335	pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP)	318:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	0	6	theme	system	101:106	arg1	properties					108:117	batter system properties	94:117	batter system properties	94:117	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.					
33964661	0	7	from	Impact	0:5	arg1	properties					108:117	batter system properties	94:117	batter system properties	94:117	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.					
33964661	7	8	theme	rye	1067:1069	arg1	viscosity					1077:1085	rye flour viscosity	1067:1085	rye flour viscosity	1067:1085	Thus, rye flour viscosity dramatically increased through interactive effects of PA on rye proteins and NSP, which could expand its food applications.					
33964661	4	9	theme	isolated	706:713	arg1	systems					730:736	their isolated protein-starch systems	700:736	their isolated protein-starch systems	700:736	Interestingly, viscosity peaked distinctively ~75 °C in PA-treated rye and barley flours, and their isolated protein-starch systems, indicating prolamins unravelled and complexed with PA during heating.					
33964661	0	10	theme	batter	94:99	arg1	properties					108:117	batter system properties	94:117	batter system properties	94:117	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.					
33964661	3	11	theme	RVA	531:533	arg1	viscosity					540:548	RVA peak viscosity	531:548	RVA peak viscosity	531:548	PA significantly increased batter viscosity, stability, and RVA peak viscosity in rye and barley flours (2.8× and 1.2×, respectively).					
33964661	2	12	theme	PA	291:292	arg1	effect					294:299	PA effect	291:299	PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP)	291:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	6	13	theme	barley	948:953	arg1	β-glucans					955:963	barley β-glucans	948:963	barley β-glucans	948:963	Furthermore, water-soluble rye NSP and arabinoxylans, but not barley β-glucans, significantly increased starch pasting viscosity with PA; oxidative gelation was not a factor.					
33964661	4	14	theme	PA-treated	662:671	arg1	rye					673:675	PA-treated rye	662:675	PA-treated rye	662:675	Interestingly, viscosity peaked distinctively ~75 °C in PA-treated rye and barley flours, and their isolated protein-starch systems, indicating prolamins unravelled and complexed with PA during heating.					
33964661	7	15	theme	PA	1141:1142	arg1	effects					1130:1136	interactive effects	1118:1136	interactive effects of PA on rye proteins and NSP	1118:1166	Thus, rye flour viscosity dramatically increased through interactive effects of PA on rye proteins and NSP, which could expand its food applications.					
33964661	2	16	theme	non-starch	437:446	arg1	polysaccharides					448:462	non-starch polysaccharides	437:462	non-starch polysaccharides (NSP)	437:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	16	theme	non-starch	437:446	arg1	NSP					465:467	NSP	465:467	NSP	465:467	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	5	17	theme	protein	865:871	arg1	composition					873:883	its protein composition	861:883	its protein composition	861:883	Oat was largely unaffected by PA, likely because of its protein composition.					
33964661	2	18	theme	pasting	318:324	arg1	properties					326:335	pasting properties	318:335	pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP)	318:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	3	19	dep	flours	568:573	arg1	1.2×					585:588	1.2×	585:588	1.2×	585:588	PA significantly increased batter viscosity, stability, and RVA peak viscosity in rye and barley flours (2.8× and 1.2×, respectively).					
33964661	3	19	dep	flours	568:573	arg1	2.8×					576:579	2.8×	576:579	2.8×	576:579	PA significantly increased batter viscosity, stability, and RVA peak viscosity in rye and barley flours (2.8× and 1.2×, respectively).					
33964661	7	20	theme	rye	1147:1149	arg1	proteins					1151:1158	rye proteins	1147:1158	rye proteins	1147:1158	Thus, rye flour viscosity dramatically increased through interactive effects of PA on rye proteins and NSP, which could expand its food applications.					
33964661	3	21	theme	rye	553:555	arg1	flours					568:573	rye and barley flours	553:573	rye and barley flours (2.8× and 1.2×, respectively)	553:603	PA significantly increased batter viscosity, stability, and RVA peak viscosity in rye and barley flours (2.8× and 1.2×, respectively).					
33964661	2	22	theme	flours	361:366	arg1	viscosity					304:312	viscosity	304:312	viscosity	304:312	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	22	theme	flours	361:366	arg1	properties					326:335	pasting properties	318:335	pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP)	318:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	0	23	theme	tannin	20:25	arg1	interactions					27:38	condensed tannin interactions	10:38	condensed tannin interactions with grain proteins and non-starch polysaccharides	10:89	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.					
33964661	3	24	theme	peak	535:538	arg1	viscosity					540:548	RVA peak viscosity	531:548	RVA peak viscosity	531:548	PA significantly increased batter viscosity, stability, and RVA peak viscosity in rye and barley flours (2.8× and 1.2×, respectively).					
33964661	2	25	theme	PA	403:404	arg1	interactions					406:417	PA interactions	403:417	PA interactions with proteins and non-starch polysaccharides (NSP)	403:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	26	theme	interactions	406:417	arg1	barley					340:345	barley	340:345	barley	340:345	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	26	theme	interactions	406:417	arg1	contributions					386:398	the relative contributions	373:398	the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP)	373:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	26	theme	interactions	406:417	arg1	flours					361:366	oat flours	357:366	oat flours	357:366	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	26	theme	interactions	406:417	arg1	rye					348:350	rye	348:350	rye	348:350	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	3	27	theme	batter	498:503	arg1	viscosity					505:513	batter viscosity	498:513	batter viscosity	498:513	PA significantly increased batter viscosity, stability, and RVA peak viscosity in rye and barley flours (2.8× and 1.2×, respectively).					
33964661	4	28	dep	prolamins	750:758	arg1	complexed					775:783	complexed	775:783	complexed with PA during heating	775:806	Interestingly, viscosity peaked distinctively ~75 °C in PA-treated rye and barley flours, and their isolated protein-starch systems, indicating prolamins unravelled and complexed with PA during heating.					
33964661	4	28	dep	prolamins	750:758	arg1	unravelled					760:769	unravelled	760:769	unravelled	760:769	Interestingly, viscosity peaked distinctively ~75 °C in PA-treated rye and barley flours, and their isolated protein-starch systems, indicating prolamins unravelled and complexed with PA during heating.					
33964661	7	29	theme	interactive	1118:1128	arg1	effects					1130:1136	interactive effects	1118:1136	interactive effects of PA on rye proteins and NSP	1118:1166	Thus, rye flour viscosity dramatically increased through interactive effects of PA on rye proteins and NSP, which could expand its food applications.					
33964661	1	30	theme	batter	201:206	arg1	viscosity					208:216	batter viscosity	201:216	batter viscosity	201:216	Proanthocyanidins (PA) cross-link wheat gluten proteins and dramatically enhance batter viscosity; PA could similarly affect related grains.					
33964661	2	31	theme	contributions	386:398	arg1	viscosity					304:312	viscosity	304:312	viscosity	304:312	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	31	theme	contributions	386:398	arg1	properties					326:335	pasting properties	318:335	pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP)	318:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	0	32	theme	interactions	27:38	arg1	Impact					0:5	Impact	0:5	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.	0:118	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.					
33964661	6	33	theme	starch	990:995	arg1	viscosity					1005:1013	starch pasting viscosity	990:1013	starch pasting viscosity	990:1013	Furthermore, water-soluble rye NSP and arabinoxylans, but not barley β-glucans, significantly increased starch pasting viscosity with PA; oxidative gelation was not a factor.					
33964661	2	34	theme	relative	377:384	arg1	contributions					386:398	the relative contributions	373:398	the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP)	373:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	35	with	interactions	406:417	arg1	proteins					424:431	proteins	424:431	proteins	424:431	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	35	with	interactions	406:417	arg1	polysaccharides					448:462	non-starch polysaccharides	437:462	non-starch polysaccharides (NSP)	437:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	35	with	interactions	406:417	arg1	NSP					465:467	NSP	465:467	NSP	465:467	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	4	36	theme	barley	681:686	arg1	flours					688:693	PA-treated rye and barley flours	662:693	PA-treated rye and barley flours	662:693	Interestingly, viscosity peaked distinctively ~75 °C in PA-treated rye and barley flours, and their isolated protein-starch systems, indicating prolamins unravelled and complexed with PA during heating.					
33964661	0	37	theme	grain	45:49	arg1	proteins					51:58	grain proteins	45:58	grain proteins	45:58	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.					
33964661	6	38	theme	rye	913:915	arg1	NSP					917:919	water-soluble rye NSP	899:919	water-soluble rye NSP	899:919	Furthermore, water-soluble rye NSP and arabinoxylans, but not barley β-glucans, significantly increased starch pasting viscosity with PA; oxidative gelation was not a factor.					
33964661	3	39	theme	barley	561:566	arg1	flours					568:573	rye and barley flours	553:573	rye and barley flours (2.8× and 1.2×, respectively)	553:603	PA significantly increased batter viscosity, stability, and RVA peak viscosity in rye and barley flours (2.8× and 1.2×, respectively).					
33964661	2	40	theme	barley	340:345	arg1	viscosity					304:312	viscosity	304:312	viscosity	304:312	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	40	theme	barley	340:345	arg1	properties					326:335	pasting properties	318:335	pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP)	318:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	6	41	theme	water-soluble	899:911	arg1	NSP					917:919	water-soluble rye NSP	899:919	water-soluble rye NSP	899:919	Furthermore, water-soluble rye NSP and arabinoxylans, but not barley β-glucans, significantly increased starch pasting viscosity with PA; oxidative gelation was not a factor.					
33964661	4	42	theme	protein-starch	715:728	arg1	systems					730:736	their isolated protein-starch systems	700:736	their isolated protein-starch systems	700:736	Interestingly, viscosity peaked distinctively ~75 °C in PA-treated rye and barley flours, and their isolated protein-starch systems, indicating prolamins unravelled and complexed with PA during heating.					
33964661	4	43	dep	peaked	631:636	arg1	~75 °C					652:657	~75 °C	652:657	~75 °C in PA-treated rye and barley flours, and their isolated protein-starch systems	652:736	Interestingly, viscosity peaked distinctively ~75 °C in PA-treated rye and barley flours, and their isolated protein-starch systems, indicating prolamins unravelled and complexed with PA during heating.					
33964661	2	44	from	effect	294:299	arg1	viscosity					304:312	viscosity	304:312	viscosity	304:312	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	2	44	from	effect	294:299	arg1	properties					326:335	pasting properties	318:335	pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP)	318:468	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	6	45	theme	pasting	997:1003	arg1	viscosity					1005:1013	starch pasting viscosity	990:1013	starch pasting viscosity	990:1013	Furthermore, water-soluble rye NSP and arabinoxylans, but not barley β-glucans, significantly increased starch pasting viscosity with PA; oxidative gelation was not a factor.					
33964661	0	46	theme	non-starch	64:73	arg1	polysaccharides					75:89	non-starch polysaccharides	64:89	non-starch polysaccharides	64:89	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.					
33964661	6	47	theme	oxidative	1024:1032	arg1	factor					1053:1058	a factor	1051:1058	a factor	1051:1058	Furthermore, water-soluble rye NSP and arabinoxylans, but not barley β-glucans, significantly increased starch pasting viscosity with PA; oxidative gelation was not a factor.					
33964661	6	47	theme	oxidative	1024:1032	arg1	gelation					1034:1041	oxidative gelation	1024:1041	oxidative gelation	1024:1041	Furthermore, water-soluble rye NSP and arabinoxylans, but not barley β-glucans, significantly increased starch pasting viscosity with PA; oxidative gelation was not a factor.					
33964661	2	48	theme	oat	357:359	arg1	flours					361:366	oat flours	357:366	oat flours	357:366	This study aimed to determine PA effect on viscosity and pasting properties of barley, rye, and oat flours, and the relative contributions of PA interactions with proteins and non-starch polysaccharides (NSP).					
33964661	1	49	theme	related	245:251	arg1	grains					253:258	related grains	245:258	related grains	245:258	Proanthocyanidins (PA) cross-link wheat gluten proteins and dramatically enhance batter viscosity; PA could similarly affect related grains.					
33964661	0	50	theme	condensed	10:18	arg1	interactions					27:38	condensed tannin interactions	10:38	condensed tannin interactions with grain proteins and non-starch polysaccharides	10:89	Impact of condensed tannin interactions with grain proteins and non-starch polysaccharides on batter system properties.					
33964661	1	51	theme	wheat	154:158	arg1	proteins					167:174	wheat gluten proteins	154:174	wheat gluten proteins	154:174	Proanthocyanidins (PA) cross-link wheat gluten proteins and dramatically enhance batter viscosity; PA could similarly affect related grains.					
33964661	7	52	theme	food	1192:1195	arg1	applications					1197:1208	its food applications	1188:1208	its food applications	1188:1208	Thus, rye flour viscosity dramatically increased through interactive effects of PA on rye proteins and NSP, which could expand its food applications.					
34973732	3	0	theme	cell	689:692	arg1	membrane					694:701	the cell membrane	685:701	the cell membrane (evidenced using cryo-EM)	685:727	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	4	1	theme	biological	848:857	arg1	experiments					859:869	biological experiments	848:869	biological experiments	848:869	This procedure can be applied to other bifidobacterial strains, to facilitate the easy isolation and purification for biological experiments and future application in nutraceuticals.					
34973732	1	2	theme	pathogen	174:181	arg1	inhibition					183:192	pathogen inhibition	174:192	pathogen inhibition	174:192	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	4	3	from	experiments	859:869	arg1	nutraceuticals					897:910	nutraceuticals	897:910	nutraceuticals	897:910	This procedure can be applied to other bifidobacterial strains, to facilitate the easy isolation and purification for biological experiments and future application in nutraceuticals.					
34973732	5	4	theme	specific	1124:1131	arg1	effects					1152:1158	specific structure-activity effects	1124:1158	specific structure-activity effects of bifidobacteria	1124:1176	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	3	5	theme	isolation	528:536	arg1	procedure					538:546	a straightforward isolation procedure	510:546	a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM)	510:727	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	5	6	theme	structure-activity	1133:1150	arg1	effects					1152:1158	specific structure-activity effects	1124:1158	specific structure-activity effects of bifidobacteria	1124:1176	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	3	7	theme	commercial	570:579	arg1	strains					597:603	four commercial bifidobacterial strains	565:603	four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis)	565:660	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	3	8	theme	straightforward	512:526	arg1	procedure					538:546	a straightforward isolation procedure	510:546	a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM)	510:727	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	3	9	located	localized	672:680	arg2	strains					597:603	four commercial bifidobacterial strains	565:603	four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis)	565:660	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	3	9	located	localized	672:680	arg1	membrane					694:701	the cell membrane	685:701	the cell membrane (evidenced using cryo-EM)	685:727	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	5	10	theme	isolated	1095:1102	arg1	EPS					1104:1106	the isolated EPS	1091:1106	the isolated EPS	1091:1106	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	4	11	theme	easy	812:815	arg1	isolation					817:825	isolation	817:825	isolation	817:825	This procedure can be applied to other bifidobacterial strains, to facilitate the easy isolation and purification for biological experiments and future application in nutraceuticals.					
34973732	1	12	theme	immune	198:203	arg1	system					205:210	immune system	198:210	immune system	198:210	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	3	13	dep	strains	597:603	arg1	infantis					652:659	B. infantis	649:659	B. infantis	649:659	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	3	13	dep	strains	597:603	arg1	adolescentis					609:620	B. adolescentis	606:620	B. adolescentis	606:620	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	3	13	dep	strains	597:603	arg1	bifidum					626:632	B. bifidum	623:632	B. bifidum	623:632	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	3	13	dep	strains	597:603	arg1	breve					638:642	B. breve	635:642	B. breve	635:642	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	5	14	theme	EPS	1104:1106	arg1	potential					1078:1086	the potential	1074:1086	the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria	1074:1176	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	3	15	theme	strains	597:603	arg1	EPS					558:560	EPS	558:560	EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM)	558:727	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	0	16	theme	Efficient	0:8	arg1	isolation					10:18	Efficient isolation	0:18	Efficient isolation of membrane-associated exopolysaccharides of four commercial bifidobacterial strains	0:103	Efficient isolation of membrane-associated exopolysaccharides of four commercial bifidobacterial strains.					
34973732	1	17	theme	many	128:131	arg1	system					205:210	immune system	198:210	immune system	198:210	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	1	17	theme	many	128:131	arg1	inhibition					183:192	pathogen inhibition	174:192	pathogen inhibition	174:192	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	1	17	theme	many	128:131	arg1	digestion					163:171	fiber digestion	157:171	fiber digestion	157:171	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	1	17	theme	many	128:131	arg1	effects					140:146	many health effects	128:146	many health effects	128:146	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	0	18	theme	membrane-associated	23:41	arg1	exopolysaccharides					43:60	membrane-associated exopolysaccharides	23:60	membrane-associated exopolysaccharides of four commercial bifidobacterial strains	23:103	Efficient isolation of membrane-associated exopolysaccharides of four commercial bifidobacterial strains.					
34973732	1	19	dep	digestion	163:171	arg1	maturation					212:221	maturation	212:221	maturation	212:221	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	2	20	theme	health	339:344	arg1	effects					346:352	important health effects	329:352	important health effects	329:352	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	5	21	theme	structural	941:950	arg1	differences					952:962	structural differences	941:962	structural differences in the EPS of the four bifidobacterial strains	941:1009	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	3	22	theme	bifidobacterial	581:595	arg1	strains					597:603	four commercial bifidobacterial strains	565:603	four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis)	565:660	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	2	23	theme	localization	432:443	arg1	knowledge					411:419	knowledge	411:419	knowledge of the EPS localization, which is important for efficient EPS isolation	411:491	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	5	24	theme	strains	1003:1009	arg1	EPS					971:973	the EPS	967:973	the EPS of the four bifidobacterial strains	967:1009	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	1	25	theme	health	133:138	arg1	system					205:210	immune system	198:210	immune system	198:210	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	1	25	theme	health	133:138	arg1	inhibition					183:192	pathogen inhibition	174:192	pathogen inhibition	174:192	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	1	25	theme	health	133:138	arg1	digestion					163:171	fiber digestion	157:171	fiber digestion	157:171	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	1	25	theme	health	133:138	arg1	effects					140:146	many health effects	128:146	many health effects	128:146	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	2	26	theme	EPS	428:430	arg1	important					455:463	important	455:463	important	455:463	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	2	26	theme	EPS	428:430	arg1	localization					432:443	the EPS localization	424:443	the EPS localization	424:443	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	0	27	theme	exopolysaccharides	43:60	arg1	isolation					10:18	Efficient isolation	0:18	Efficient isolation of membrane-associated exopolysaccharides of four commercial bifidobacterial strains	0:103	Efficient isolation of membrane-associated exopolysaccharides of four commercial bifidobacterial strains.					
34973732	3	28	dep	membrane	694:701	arg1	evidenced					704:712	evidenced	704:712	evidenced using cryo-EM	704:726	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	2	29	theme	EPS	479:481	arg1	isolation					483:491	efficient EPS isolation	469:491	efficient EPS isolation	469:491	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	0	30	theme	bifidobacterial	81:95	arg1	strains					97:103	four commercial bifidobacterial strains	65:103	four commercial bifidobacterial strains	65:103	Efficient isolation of membrane-associated exopolysaccharides of four commercial bifidobacterial strains.					
34973732	2	31	theme	thorough	365:372	arg1	investigation					374:386	their thorough investigation	359:386	their thorough investigation	359:386	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	2	32	theme	efficient	469:477	arg1	isolation					483:491	efficient EPS isolation	469:491	efficient EPS isolation	469:491	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	5	33	theme	bifidobacteria	1163:1176	arg1	effects					1152:1158	specific structure-activity effects	1124:1158	specific structure-activity effects of bifidobacteria	1124:1176	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	4	34	theme	future	875:880	arg1	application					882:892	future application	875:892	future application in nutraceuticals	875:910	This procedure can be applied to other bifidobacterial strains, to facilitate the easy isolation and purification for biological experiments and future application in nutraceuticals.					
34973732	5	35	theme	monosaccharide	1024:1037	arg1	composition					1039:1049	monosaccharide composition	1024:1049	monosaccharide composition	1024:1049	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	5	36	theme	composition	1039:1049	arg1	terms					1015:1019	terms	1015:1019	terms of monosaccharide composition and size	1015:1058	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	4	37	theme	bifidobacterial	769:783	arg1	strains					785:791	other bifidobacterial strains	763:791	other bifidobacterial strains	763:791	This procedure can be applied to other bifidobacterial strains, to facilitate the easy isolation and purification for biological experiments and future application in nutraceuticals.					
34973732	4	38	theme	other	763:767	arg1	strains					785:791	other bifidobacterial strains	763:791	other bifidobacterial strains	763:791	This procedure can be applied to other bifidobacterial strains, to facilitate the easy isolation and purification for biological experiments and future application in nutraceuticals.					
34973732	3	39	theme	B.	635:636	arg1	breve					638:642	B. breve	635:642	B. breve	635:642	Here we present a straightforward isolation procedure to obtain EPS of four commercial bifidobacterial strains (B. adolescentis, B. bifidum, B. breve, and B. infantis), that are localized at the cell membrane (evidenced using cryo-EM).					
34973732	2	40	theme	important	329:337	arg1	effects					346:352	important health effects	329:352	important health effects	329:352	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	1	41	theme	newborn	242:248	arg1	infant					250:255	the newborn infant	238:255	the newborn infant	238:255	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	4	42	from	application	882:892	arg1	nutraceuticals					897:910	nutraceuticals	897:910	nutraceuticals	897:910	This procedure can be applied to other bifidobacterial strains, to facilitate the easy isolation and purification for biological experiments and future application in nutraceuticals.					
34973732	1	43	theme	fiber	157:161	arg1	digestion					163:171	fiber digestion	157:171	fiber digestion	157:171	Bifidobacteria confer many health effects, such as fiber digestion, pathogen inhibition and immune system maturation, especially in the newborn infant.					
34973732	0	44	theme	strains	97:103	arg1	exopolysaccharides					43:60	membrane-associated exopolysaccharides	23:60	membrane-associated exopolysaccharides of four commercial bifidobacterial strains	23:103	Efficient isolation of membrane-associated exopolysaccharides of four commercial bifidobacterial strains.					
34973732	5	45	theme	size	1055:1058	arg1	terms					1015:1019	terms	1015:1019	terms of monosaccharide composition and size	1015:1058	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	5	46	theme	bifidobacterial	987:1001	arg1	strains					1003:1009	the four bifidobacterial strains	978:1009	the four bifidobacterial strains	978:1009	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34973732	2	47	theme	knowledge	411:419	arg1	lack					403:406	lack	403:406	lack of knowledge of the EPS localization, which is important for efficient EPS isolation	403:491	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	0	48	theme	commercial	70:79	arg1	strains					97:103	four commercial bifidobacterial strains	65:103	four commercial bifidobacterial strains	65:103	Efficient isolation of membrane-associated exopolysaccharides of four commercial bifidobacterial strains.					
34973732	2	49	theme	bifidobacterial	262:276	arg1	exopolysaccharides					278:295	The bifidobacterial exopolysaccharides	258:295	The bifidobacterial exopolysaccharides (EPS)	258:301	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	2	49	theme	bifidobacterial	262:276	arg1	EPS					298:300	EPS	298:300	EPS	298:300	The bifidobacterial exopolysaccharides (EPS) are often associated with important health effects, but their thorough investigation is hampered by lack of knowledge of the EPS localization, which is important for efficient EPS isolation.					
34973732	5	50	from	differences	952:962	arg1	EPS					971:973	the EPS	967:973	the EPS of the four bifidobacterial strains	967:1009	In addition, we demonstrate structural differences in the EPS of the four bifidobacterial strains, in terms of monosaccharide composition and size, highlighting the potential of the isolated EPS for determining specific structure-activity effects of bifidobacteria.					
34474846	5	0	theme	layers	741:746	arg1	duration					752:759	duration	752:759	duration of mineralization	752:777	By adjusting the number of the layers and duration of mineralization, the nanoscale morphology of composite fibers was optimized.					
34474846	5	0	theme	layers	741:746	arg1	number					727:732	the number	723:732	the number of the layers	723:746	By adjusting the number of the layers and duration of mineralization, the nanoscale morphology of composite fibers was optimized.					
34474846	7	1	theme	composite	1170:1178	arg1	membrane					1186:1193	this composite fiber membrane	1165:1193	this composite fiber membrane	1165:1193	In a subcutaneous implantation in mice, this composite fiber membrane enhanced in vivo ectopic osteogenesis.					
34474846	7	2	theme	fiber	1180:1184	arg1	membrane					1186:1193	this composite fiber membrane	1165:1193	this composite fiber membrane	1165:1193	In a subcutaneous implantation in mice, this composite fiber membrane enhanced in vivo ectopic osteogenesis.					
34474846	1	3	theme	osteoinductive	189:202	arg1	scaffold					215:222	osteoinductive artificial scaffold	189:222	osteoinductive artificial scaffold for bone repair and regeneration	189:255	It is of great significance to develop osteoinductive artificial scaffold for bone repair and regeneration.					
34474846	0	4	theme	bone	131:134	arg1	regeneration					136:147	bone regeneration	131:147	bone regeneration	131:147	Biofunctionalization of electrospun fiber membranes by LbL-collagen/chondroitin sulfate nanocoating followed by mineralization for bone regeneration.					
34474846	4	5	theme	apatite	690:696	arg1	deposition					698:707	apatite deposition	690:707	apatite deposition	690:707	The fibers coated with the multilayer components served as the precursor matrix to induce apatite deposition.					
34474846	6	6	dep	adhesion	1050:1057	arg1	the					1046:1048	the	1046:1048	the	1046:1048	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	8	7	theme	bone	1323:1326	arg1	matrix					1328:1333	the bone matrix	1319:1333	the bone matrix	1319:1333	Our nano-architectural scaffolds were able to mimic the composition and structure of the bone matrix to a certain extent, holding great potential for bone repair and regeneration.					
34474846	6	8	dep	collagen	865:872	arg1	the					861:863	the	861:863	the	861:863	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	1	9	theme	artificial	204:213	arg1	scaffold					215:222	osteoinductive artificial scaffold	189:222	osteoinductive artificial scaffold for bone repair and regeneration	189:255	It is of great significance to develop osteoinductive artificial scaffold for bone repair and regeneration.					
34474846	6	10	theme	composite	1003:1011	arg1	scaffolds					1013:1021	the obtained polycaprolactone-apatite composite scaffolds	965:1021	the obtained polycaprolactone-apatite composite scaffolds	965:1021	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	8	11	dep	composition	1290:1300	arg1	the					1286:1288	the	1286:1288	the	1286:1288	Our nano-architectural scaffolds were able to mimic the composition and structure of the bone matrix to a certain extent, holding great potential for bone repair and regeneration.					
34474846	6	12	theme	sulfate	890:896	arg1	bilayers					849:856	ten bilayers	845:856	ten bilayers of the collagen and chondroitin sulfate	845:896	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	2	13	theme	polycaprolactone	319:334	arg1	fibers					336:341	electrospun polycaprolactone fibers	307:341	electrospun polycaprolactone fibers	307:341	We constructed a biomimetic apatite interface on electrospun polycaprolactone fibers by combining layer-by-layer (LbL) nanocoating with mineralization to fabricate an osteoinductive artificial scaffold.					
34474846	3	14	theme	chondroitin	531:541	arg1	sulfate					543:549	anionic chondroitin sulfate	523:549	anionic chondroitin sulfate	523:549	After polydopamine modification, cationic type-І collagen and anionic chondroitin sulfate were sequentially adsorbed on the fiber surface.					
34474846	6	15	theme	polycaprolactone-apatite	978:1001	arg1	scaffolds					1013:1021	the obtained polycaprolactone-apatite composite scaffolds	965:1021	the obtained polycaprolactone-apatite composite scaffolds	965:1021	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	6	16	theme	chondroitin	878:888	arg1	sulfate					890:896	chondroitin sulfate	878:896	chondroitin sulfate	878:896	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	3	17	theme	polydopamine	467:478	arg1	modification					480:491	polydopamine modification	467:491	polydopamine modification	467:491	After polydopamine modification, cationic type-І collagen and anionic chondroitin sulfate were sequentially adsorbed on the fiber surface.					
34474846	6	18	theme	cells	1118:1122	arg1	proliferation					1060:1072	proliferation	1060:1072	proliferation	1060:1072	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	6	18	theme	cells	1118:1122	arg1	adhesion					1050:1057	adhesion	1050:1057	adhesion	1050:1057	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	6	18	theme	cells	1118:1122	arg1	differentiation					1090:1104	osteogenic differentiation	1079:1104	osteogenic differentiation	1079:1104	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	7	19	theme	in	1204:1205	arg1	osteogenesis					1220:1231	in vivo ectopic osteogenesis	1204:1231	in vivo ectopic osteogenesis	1204:1231	In a subcutaneous implantation in mice, this composite fiber membrane enhanced in vivo ectopic osteogenesis.					
34474846	2	20	from	interface	294:302	arg1	fibers					336:341	electrospun polycaprolactone fibers	307:341	electrospun polycaprolactone fibers	307:341	We constructed a biomimetic apatite interface on electrospun polycaprolactone fibers by combining layer-by-layer (LbL) nanocoating with mineralization to fabricate an osteoinductive artificial scaffold.					
34474846	1	21	theme	bone	228:231	arg1	repair					233:238	bone repair	228:238	bone repair	228:238	It is of great significance to develop osteoinductive artificial scaffold for bone repair and regeneration.					
34474846	0	22	theme	fiber	36:40	arg1	membranes					42:50	electrospun fiber membranes	24:50	electrospun fiber membranes	24:50	Biofunctionalization of electrospun fiber membranes by LbL-collagen/chondroitin sulfate nanocoating followed by mineralization for bone regeneration.					
34474846	6	23	theme	MC3T3-E1	1109:1116	arg1	cells					1118:1122	MC3T3-E1 cells	1109:1122	MC3T3-E1 cells	1109:1122	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	6	24	theme	collagen	865:872	arg1	bilayers					849:856	ten bilayers	845:856	ten bilayers of the collagen and chondroitin sulfate	845:896	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	7	25	from	implantation	1143:1154	arg1	mice					1159:1162	mice	1159:1162	mice	1159:1162	In a subcutaneous implantation in mice, this composite fiber membrane enhanced in vivo ectopic osteogenesis.					
34474846	0	26	theme	electrospun	24:34	arg1	membranes					42:50	electrospun fiber membranes	24:50	electrospun fiber membranes	24:50	Biofunctionalization of electrospun fiber membranes by LbL-collagen/chondroitin sulfate nanocoating followed by mineralization for bone regeneration.					
34474846	4	27	theme	precursor	663:671	arg1	matrix					673:678	the precursor matrix	659:678	the precursor matrix	659:678	The fibers coated with the multilayer components served as the precursor matrix to induce apatite deposition.					
34474846	4	27	theme	precursor	663:671	arg1	fibers					604:609	The fibers	600:609	The fibers coated with the multilayer components	600:647	The fibers coated with the multilayer components served as the precursor matrix to induce apatite deposition.					
34474846	7	28	theme	ectopic	1212:1218	arg1	osteogenesis					1220:1231	in vivo ectopic osteogenesis	1204:1231	in vivo ectopic osteogenesis	1204:1231	In a subcutaneous implantation in mice, this composite fiber membrane enhanced in vivo ectopic osteogenesis.					
34474846	6	29	theme	obtained	969:976	arg1	scaffolds					1013:1021	the obtained polycaprolactone-apatite composite scaffolds	965:1021	the obtained polycaprolactone-apatite composite scaffolds	965:1021	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	5	30	theme	fibers	818:823	arg1	morphology					794:803	the nanoscale morphology	780:803	the nanoscale morphology of composite fibers	780:823	By adjusting the number of the layers and duration of mineralization, the nanoscale morphology of composite fibers was optimized.					
34474846	3	31	theme	cationic	494:501	arg1	collagen					510:517	cationic type-І collagen	494:517	cationic type-І collagen	494:517	After polydopamine modification, cationic type-І collagen and anionic chondroitin sulfate were sequentially adsorbed on the fiber surface.					
34474846	5	32	theme	composite	808:816	arg1	fibers					818:823	composite fibers	808:823	composite fibers	808:823	By adjusting the number of the layers and duration of mineralization, the nanoscale morphology of composite fibers was optimized.					
34474846	2	33	theme	biomimetic	275:284	arg1	interface					294:302	a biomimetic apatite interface	273:302	a biomimetic apatite interface on electrospun polycaprolactone fibers	273:341	We constructed a biomimetic apatite interface on electrospun polycaprolactone fibers by combining layer-by-layer (LbL) nanocoating with mineralization to fabricate an osteoinductive artificial scaffold.					
34474846	8	34	theme	bone	1384:1387	arg1	repair					1389:1394	bone repair	1384:1394	bone repair	1384:1394	Our nano-architectural scaffolds were able to mimic the composition and structure of the bone matrix to a certain extent, holding great potential for bone repair and regeneration.					
34474846	3	35	theme	type-І	503:508	arg1	collagen					510:517	cationic type-І collagen	494:517	cationic type-І collagen	494:517	After polydopamine modification, cationic type-І collagen and anionic chondroitin sulfate were sequentially adsorbed on the fiber surface.					
34474846	6	36	theme	osteogenic	1079:1088	arg1	differentiation					1090:1104	osteogenic differentiation	1079:1104	osteogenic differentiation	1079:1104	When ten bilayers of the collagen and chondroitin sulfate were deposited onto the fibers followed by one day-mineralization, the obtained polycaprolactone-apatite composite scaffolds significantly promoted the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.					
34474846	0	37	theme	membranes	42:50	arg1	Biofunctionalization					0:19	Biofunctionalization	0:19	Biofunctionalization of electrospun fiber membranes by LbL-collagen/chondroitin sulfate nanocoating	0:98	Biofunctionalization of electrospun fiber membranes by LbL-collagen/chondroitin sulfate nanocoating followed by mineralization for bone regeneration.					
34474846	8	38	theme	certain	1340:1346	arg1	extent					1348:1353	a certain extent	1338:1353	a certain extent	1338:1353	Our nano-architectural scaffolds were able to mimic the composition and structure of the bone matrix to a certain extent, holding great potential for bone repair and regeneration.					
34474846	0	39	theme	sulfate	80:86	arg1	nanocoating					88:98	LbL-collagen/chondroitin sulfate nanocoating	55:98	LbL-collagen/chondroitin sulfate nanocoating	55:98	Biofunctionalization of electrospun fiber membranes by LbL-collagen/chondroitin sulfate nanocoating followed by mineralization for bone regeneration.					
34474846	5	40	theme	mineralization	764:777	arg1	duration					752:759	duration	752:759	duration of mineralization	752:777	By adjusting the number of the layers and duration of mineralization, the nanoscale morphology of composite fibers was optimized.					
34474846	5	40	theme	mineralization	764:777	arg1	number					727:732	the number	723:732	the number of the layers	723:746	By adjusting the number of the layers and duration of mineralization, the nanoscale morphology of composite fibers was optimized.					
34474846	3	41	theme	anionic	523:529	arg1	sulfate					543:549	anionic chondroitin sulfate	523:549	anionic chondroitin sulfate	523:549	After polydopamine modification, cationic type-І collagen and anionic chondroitin sulfate were sequentially adsorbed on the fiber surface.					
34474846	3	42	theme	fiber	585:589	arg1	surface					591:597	the fiber surface	581:597	the fiber surface	581:597	After polydopamine modification, cationic type-І collagen and anionic chondroitin sulfate were sequentially adsorbed on the fiber surface.					
34474846	0	43	theme	LbL-collagen/chondroitin	55:78	arg1	nanocoating					88:98	LbL-collagen/chondroitin sulfate nanocoating	55:98	LbL-collagen/chondroitin sulfate nanocoating	55:98	Biofunctionalization of electrospun fiber membranes by LbL-collagen/chondroitin sulfate nanocoating followed by mineralization for bone regeneration.					
34474846	4	44	theme	multilayer	627:636	arg1	components					638:647	the multilayer components	623:647	the multilayer components	623:647	The fibers coated with the multilayer components served as the precursor matrix to induce apatite deposition.					
34474846	8	45	theme	nano-architectural	1238:1255	arg1	scaffolds					1257:1265	Our nano-architectural scaffolds	1234:1265	Our nano-architectural scaffolds	1234:1265	Our nano-architectural scaffolds were able to mimic the composition and structure of the bone matrix to a certain extent, holding great potential for bone repair and regeneration.					
34474846	2	46	theme	electrospun	307:317	arg1	fibers					336:341	electrospun polycaprolactone fibers	307:341	electrospun polycaprolactone fibers	307:341	We constructed a biomimetic apatite interface on electrospun polycaprolactone fibers by combining layer-by-layer (LbL) nanocoating with mineralization to fabricate an osteoinductive artificial scaffold.					
34474846	5	47	theme	nanoscale	784:792	arg1	morphology					794:803	the nanoscale morphology	780:803	the nanoscale morphology of composite fibers	780:823	By adjusting the number of the layers and duration of mineralization, the nanoscale morphology of composite fibers was optimized.					
34474846	8	48	theme	matrix	1328:1333	arg1	composition					1290:1300	composition	1290:1300	composition	1290:1300	Our nano-architectural scaffolds were able to mimic the composition and structure of the bone matrix to a certain extent, holding great potential for bone repair and regeneration.					
34474846	8	48	theme	matrix	1328:1333	arg1	structure					1306:1314	structure	1306:1314	structure	1306:1314	Our nano-architectural scaffolds were able to mimic the composition and structure of the bone matrix to a certain extent, holding great potential for bone repair and regeneration.					
34474846	1	49	theme	great	159:163	arg1	significance					165:176	great significance	159:176	great significance	159:176	It is of great significance to develop osteoinductive artificial scaffold for bone repair and regeneration.					
34474846	8	50	theme	great	1364:1368	arg1	potential					1370:1378	great potential	1364:1378	great potential for bone repair and regeneration	1364:1411	Our nano-architectural scaffolds were able to mimic the composition and structure of the bone matrix to a certain extent, holding great potential for bone repair and regeneration.					
34474846	7	51	theme	subcutaneous	1130:1141	arg1	implantation					1143:1154	a subcutaneous implantation	1128:1154	a subcutaneous implantation in mice	1128:1162	In a subcutaneous implantation in mice, this composite fiber membrane enhanced in vivo ectopic osteogenesis.					
34474846	2	52	theme	artificial	440:449	arg1	scaffold					451:458	an osteoinductive artificial scaffold	422:458	an osteoinductive artificial scaffold	422:458	We constructed a biomimetic apatite interface on electrospun polycaprolactone fibers by combining layer-by-layer (LbL) nanocoating with mineralization to fabricate an osteoinductive artificial scaffold.					
34474846	7	53	dep	in	1204:1205	arg1	vivo					1207:1210	vivo	1207:1210	vivo	1207:1210	In a subcutaneous implantation in mice, this composite fiber membrane enhanced in vivo ectopic osteogenesis.					
34474846	2	54	theme	apatite	286:292	arg1	interface					294:302	a biomimetic apatite interface	273:302	a biomimetic apatite interface on electrospun polycaprolactone fibers	273:341	We constructed a biomimetic apatite interface on electrospun polycaprolactone fibers by combining layer-by-layer (LbL) nanocoating with mineralization to fabricate an osteoinductive artificial scaffold.					
34474846	2	55	theme	osteoinductive	425:438	arg1	scaffold					451:458	an osteoinductive artificial scaffold	422:458	an osteoinductive artificial scaffold	422:458	We constructed a biomimetic apatite interface on electrospun polycaprolactone fibers by combining layer-by-layer (LbL) nanocoating with mineralization to fabricate an osteoinductive artificial scaffold.					
33142624	1	0	theme	accompanying	268:279	arg1	side-effects					281:292	accompanying side-effects	268:292	accompanying side-effects	268:292	It seems quite necessary for obtaining effective substances from natural products against the diabetic nephropathic (DN) with the presently clinical problems of accompanying side-effects and lowing life qualities.					
33142624	2	1	theme	Coprinus	378:385	arg1	CMP					421:423	CMP	421:423	CMP	421:423	This work aimed to characterize the primary structure of Coprinus comatus mycelium polysaccharides (CMP) and investigate the abilities against DN in streptozotocin induced mice models.					
33142624	2	1	theme	Coprinus	378:385	arg1	polysaccharides					404:418	Coprinus comatus mycelium polysaccharides	378:418	Coprinus comatus mycelium polysaccharides (CMP)	378:424	This work aimed to characterize the primary structure of Coprinus comatus mycelium polysaccharides (CMP) and investigate the abilities against DN in streptozotocin induced mice models.					
33142624	1	2	theme	side-effects	281:292	arg1	problems					256:263	the presently clinical problems	233:263	the presently clinical problems of accompanying side-effects	233:292	It seems quite necessary for obtaining effective substances from natural products against the diabetic nephropathic (DN) with the presently clinical problems of accompanying side-effects and lowing life qualities.					
33142624	0	3	from	Characterization	0:15	arg1	comatus					98:104	Coprinus comatus	89:104	Coprinus comatus	89:104	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	4	4	theme	PTEN/PI3K/Akt	803:815	arg1	pathways					837:844	the PTEN/PI3K/Akt and Wnt-1/β-catenin pathways	799:844	the PTEN/PI3K/Akt and Wnt-1/β-catenin pathways	799:844	Besides, the western blot results suggested that CMP reversed renal injury by modulating the PTEN/PI3K/Akt and Wnt-1/β-catenin pathways.					
33142624	3	5	from	inflammation	685:696	arg1	mice					704:707	DN mice	701:707	DN mice	701:707	The results indicated that CMP could improve insulin resistance and energy metabolism, and significantly suppress dysfunction on kidney and relieve the renal oxidative stress and inflammation in DN mice.					
33142624	5	6	theme	anti-diabetic	1062:1074	arg1	effects					1089:1095	the anti-diabetic nephropathic effects	1058:1095	the anti-diabetic nephropathic effects of CMP	1058:1102	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	2	7	dep	Coprinus	378:385	arg1	comatus					387:393	comatus	387:393	comatus	387:393	This work aimed to characterize the primary structure of Coprinus comatus mycelium polysaccharides (CMP) and investigate the abilities against DN in streptozotocin induced mice models.					
33142624	5	8	theme	structure	851:859	arg1	analysis					861:868	The structure analysis	847:868	The structure analysis	847:868	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	4	9	theme	Wnt-1/β-catenin	821:835	arg1	pathways					837:844	the PTEN/PI3K/Akt and Wnt-1/β-catenin pathways	799:844	the PTEN/PI3K/Akt and Wnt-1/β-catenin pathways	799:844	Besides, the western blot results suggested that CMP reversed renal injury by modulating the PTEN/PI3K/Akt and Wnt-1/β-catenin pathways.					
33142624	6	10	theme	functional	1181:1190	arg1	foods/drugs					1192:1202	functional foods/drugs	1181:1202	functional foods/drugs on preventing diabetic nephropathy	1181:1237	The results suggested that polysaccharides form C. comatus could be used as functional foods/drugs on preventing diabetic nephropathy.					
33142624	6	10	theme	functional	1181:1190	arg1	polysaccharides					1132:1146	polysaccharides	1132:1146	polysaccharides form C. comatus	1132:1162	The results suggested that polysaccharides form C. comatus could be used as functional foods/drugs on preventing diabetic nephropathy.					
33142624	0	11	theme	mycelium	59:66	arg1	polysaccharides					68:82	mycelium polysaccharides	59:82	mycelium polysaccharides from Coprinus comatus	59:104	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	5	12	theme	proper	996:1001	arg1	weights					1013:1019	proper molecular weights	996:1019	proper molecular weights of 495.8 kDa	996:1032	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	1	13	theme	clinical	247:254	arg1	problems					256:263	the presently clinical problems	233:263	the presently clinical problems of accompanying side-effects	233:292	It seems quite necessary for obtaining effective substances from natural products against the diabetic nephropathic (DN) with the presently clinical problems of accompanying side-effects and lowing life qualities.					
33142624	2	14	theme	induced	485:491	arg1	models					498:503	streptozotocin induced mice models	470:503	streptozotocin induced mice models	470:503	This work aimed to characterize the primary structure of Coprinus comatus mycelium polysaccharides (CMP) and investigate the abilities against DN in streptozotocin induced mice models.					
33142624	0	15	from	ability	48:54	arg1	comatus					98:104	Coprinus comatus	89:104	Coprinus comatus	89:104	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	6	16	used	used	1173:1176	arg2	polysaccharides					1132:1146	polysaccharides	1132:1146	polysaccharides form C. comatus	1132:1162	The results suggested that polysaccharides form C. comatus could be used as functional foods/drugs on preventing diabetic nephropathy.					
33142624	6	16	used	used	1173:1176	arg2	foods/drugs					1192:1202	functional foods/drugs	1181:1202	functional foods/drugs on preventing diabetic nephropathy	1181:1237	The results suggested that polysaccharides form C. comatus could be used as functional foods/drugs on preventing diabetic nephropathy.					
33142624	5	17	theme	kDa	1030:1032	arg1	weights					1013:1019	proper molecular weights	996:1019	proper molecular weights of 495.8 kDa	996:1032	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	5	17	theme	kDa	1030:1032	arg1	configuration					978:990	α-pyranose configuration	967:990	α-pyranose configuration	967:990	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	5	17	theme	kDa	1030:1032	arg1	monosaccharide-compositions					925:951	the major monosaccharide-compositions	915:951	the major monosaccharide-compositions of galactose	915:964	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	3	18	from	stress	674:679	arg1	mice					704:707	DN mice	701:707	DN mice	701:707	The results indicated that CMP could improve insulin resistance and energy metabolism, and significantly suppress dysfunction on kidney and relieve the renal oxidative stress and inflammation in DN mice.					
33142624	5	19	theme	galactose	956:964	arg1	weights					1013:1019	proper molecular weights	996:1019	proper molecular weights of 495.8 kDa	996:1032	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	5	19	theme	galactose	956:964	arg1	configuration					978:990	α-pyranose configuration	967:990	α-pyranose configuration	967:990	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	5	19	theme	galactose	956:964	arg1	monosaccharide-compositions					925:951	the major monosaccharide-compositions	915:951	the major monosaccharide-compositions of galactose	915:964	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	1	20	theme	life	305:308	arg1	qualities					310:318	life qualities	305:318	life qualities	305:318	It seems quite necessary for obtaining effective substances from natural products against the diabetic nephropathic (DN) with the presently clinical problems of accompanying side-effects and lowing life qualities.					
33142624	4	21	theme	renal	772:776	arg1	injury					778:783	renal injury	772:783	renal injury	772:783	Besides, the western blot results suggested that CMP reversed renal injury by modulating the PTEN/PI3K/Akt and Wnt-1/β-catenin pathways.					
33142624	3	22	theme	DN	701:702	arg1	mice					704:707	DN mice	701:707	DN mice	701:707	The results indicated that CMP could improve insulin resistance and energy metabolism, and significantly suppress dysfunction on kidney and relieve the renal oxidative stress and inflammation in DN mice.					
33142624	0	23	theme	nephropathic	35:46	arg1	ability					48:54	anti-diabetic nephropathic ability	21:54	anti-diabetic nephropathic ability	21:54	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	0	24	theme	anti-diabetic	21:33	arg1	ability					48:54	anti-diabetic nephropathic ability	21:54	anti-diabetic nephropathic ability	21:54	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	5	25	theme	nephropathic	1076:1087	arg1	effects					1089:1095	the anti-diabetic nephropathic effects	1058:1095	the anti-diabetic nephropathic effects of CMP	1058:1102	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	4	26	theme	blot	731:734	arg1	results					736:742	the western blot results	719:742	the western blot results	719:742	Besides, the western blot results suggested that CMP reversed renal injury by modulating the PTEN/PI3K/Akt and Wnt-1/β-catenin pathways.					
33142624	3	27	theme	insulin	551:557	arg1	resistance					559:568	insulin resistance	551:568	insulin resistance	551:568	The results indicated that CMP could improve insulin resistance and energy metabolism, and significantly suppress dysfunction on kidney and relieve the renal oxidative stress and inflammation in DN mice.					
33142624	5	28	with	characterizations	892:908	arg1	weights					1013:1019	proper molecular weights	996:1019	proper molecular weights of 495.8 kDa	996:1032	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	5	28	with	characterizations	892:908	arg1	configuration					978:990	α-pyranose configuration	967:990	α-pyranose configuration	967:990	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	5	28	with	characterizations	892:908	arg1	monosaccharide-compositions					925:951	the major monosaccharide-compositions	915:951	the major monosaccharide-compositions of galactose	915:964	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	0	29	from	comatus	98:104	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	0	29	from	comatus	98:104	arg1	ability					48:54	anti-diabetic nephropathic ability	21:54	anti-diabetic nephropathic ability	21:54	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	0	29	from	comatus	98:104	arg1	polysaccharides					68:82	mycelium polysaccharides	59:82	mycelium polysaccharides from Coprinus comatus	59:104	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	1	30	dep	nephropathic	210:221	arg1	DN					224:225	DN	224:225	DN	224:225	It seems quite necessary for obtaining effective substances from natural products against the diabetic nephropathic (DN) with the presently clinical problems of accompanying side-effects and lowing life qualities.					
33142624	4	31	theme	western	723:729	arg1	results					736:742	the western blot results	719:742	the western blot results	719:742	Besides, the western blot results suggested that CMP reversed renal injury by modulating the PTEN/PI3K/Akt and Wnt-1/β-catenin pathways.					
33142624	5	32	theme	major	919:923	arg1	monosaccharide-compositions					925:951	the major monosaccharide-compositions	915:951	the major monosaccharide-compositions of galactose	915:964	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	0	33	theme	polysaccharides	68:82	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	0	33	theme	polysaccharides	68:82	arg1	ability					48:54	anti-diabetic nephropathic ability	21:54	anti-diabetic nephropathic ability	21:54	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	5	34	theme	α-pyranose	967:976	arg1	configuration					978:990	α-pyranose configuration	967:990	α-pyranose configuration	967:990	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	1	35	with	nephropathic	210:221	arg1	problems					256:263	the presently clinical problems	233:263	the presently clinical problems of accompanying side-effects	233:292	It seems quite necessary for obtaining effective substances from natural products against the diabetic nephropathic (DN) with the presently clinical problems of accompanying side-effects and lowing life qualities.					
33142624	6	36	theme	diabetic	1218:1225	arg1	nephropathy					1227:1237	diabetic nephropathy	1218:1237	diabetic nephropathy	1218:1237	The results suggested that polysaccharides form C. comatus could be used as functional foods/drugs on preventing diabetic nephropathy.					
33142624	5	37	theme	molecular	1003:1011	arg1	weights					1013:1019	proper molecular weights	996:1019	proper molecular weights of 495.8 kDa	996:1032	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	2	38	theme	mice	493:496	arg1	models					498:503	streptozotocin induced mice models	470:503	streptozotocin induced mice models	470:503	This work aimed to characterize the primary structure of Coprinus comatus mycelium polysaccharides (CMP) and investigate the abilities against DN in streptozotocin induced mice models.					
33142624	3	39	theme	oxidative	664:672	arg1	stress					674:679	the renal oxidative stress	654:679	the renal oxidative stress	654:679	The results indicated that CMP could improve insulin resistance and energy metabolism, and significantly suppress dysfunction on kidney and relieve the renal oxidative stress and inflammation in DN mice.					
33142624	1	40	theme	effective	146:154	arg1	substances					156:165	effective substances	146:165	effective substances	146:165	It seems quite necessary for obtaining effective substances from natural products against the diabetic nephropathic (DN) with the presently clinical problems of accompanying side-effects and lowing life qualities.					
33142624	3	41	theme	energy	574:579	arg1	metabolism					581:590	energy metabolism	574:590	energy metabolism	574:590	The results indicated that CMP could improve insulin resistance and energy metabolism, and significantly suppress dysfunction on kidney and relieve the renal oxidative stress and inflammation in DN mice.					
33142624	5	42	theme	CMP	1100:1102	arg1	effects					1089:1095	the anti-diabetic nephropathic effects	1058:1095	the anti-diabetic nephropathic effects of CMP	1058:1102	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	1	43	theme	natural	172:178	arg1	products					180:187	natural products	172:187	natural products against the diabetic nephropathic (DN) with the presently clinical problems of accompanying side-effects	172:292	It seems quite necessary for obtaining effective substances from natural products against the diabetic nephropathic (DN) with the presently clinical problems of accompanying side-effects and lowing life qualities.					
33142624	2	44	theme	primary	357:363	arg1	structure					365:373	the primary structure	353:373	the primary structure of Coprinus comatus mycelium polysaccharides (CMP)	353:424	This work aimed to characterize the primary structure of Coprinus comatus mycelium polysaccharides (CMP) and investigate the abilities against DN in streptozotocin induced mice models.					
33142624	0	45	theme	Coprinus	89:96	arg1	comatus					98:104	Coprinus comatus	89:104	Coprinus comatus	89:104	Characterization and anti-diabetic nephropathic ability of mycelium polysaccharides from Coprinus comatus.					
33142624	5	46	theme	typical	884:890	arg1	characterizations					892:908	the typical characterizations	880:908	the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP	880:1102	The structure analysis indicated the typical characterizations with the major monosaccharide-compositions of galactose, α-pyranose configuration and proper molecular weights of 495.8 kDa possibly contributed to the anti-diabetic nephropathic effects of CMP.					
33142624	2	47	theme	polysaccharides	404:418	arg1	structure					365:373	the primary structure	353:373	the primary structure of Coprinus comatus mycelium polysaccharides (CMP)	353:424	This work aimed to characterize the primary structure of Coprinus comatus mycelium polysaccharides (CMP) and investigate the abilities against DN in streptozotocin induced mice models.					
33142624	3	48	from	dysfunction	620:630	arg1	kidney					635:640	kidney	635:640	kidney	635:640	The results indicated that CMP could improve insulin resistance and energy metabolism, and significantly suppress dysfunction on kidney and relieve the renal oxidative stress and inflammation in DN mice.					
33142624	3	49	theme	renal	658:662	arg1	stress					674:679	the renal oxidative stress	654:679	the renal oxidative stress	654:679	The results indicated that CMP could improve insulin resistance and energy metabolism, and significantly suppress dysfunction on kidney and relieve the renal oxidative stress and inflammation in DN mice.					
33142624	2	50	theme	mycelium	395:402	arg1	CMP					421:423	CMP	421:423	CMP	421:423	This work aimed to characterize the primary structure of Coprinus comatus mycelium polysaccharides (CMP) and investigate the abilities against DN in streptozotocin induced mice models.					
33142624	2	50	theme	mycelium	395:402	arg1	polysaccharides					404:418	Coprinus comatus mycelium polysaccharides	378:418	Coprinus comatus mycelium polysaccharides (CMP)	378:424	This work aimed to characterize the primary structure of Coprinus comatus mycelium polysaccharides (CMP) and investigate the abilities against DN in streptozotocin induced mice models.					
33497195	0	0	theme	Dissociation	133:144	arg1	Spectrometry					151:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry.					
33497195	4	1	theme	CTD	784:786	arg1	fragmentation					788:800	CTD fragmentation	784:800	CTD fragmentation of the pectin mixtures in data-dependent acquisition mode	784:858	This work employed CTD fragmentation of the pectin mixtures in data-dependent acquisition mode.					
33497195	1	2	theme	acid	224:227	arg1	residues					229:236	galacturonic acid residues	211:236	galacturonic acid residues	211:236	Pectins are natural polysaccharides made from galacturonic acid residues, and they are widely used as an excipient in food and pharmaceutical industries.					
33497195	8	3	theme	CTD	1319:1321	arg1	combination					1304:1314	The combination	1300:1314	The combination of CTD in real time with UHPLC	1300:1345	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	1	4	theme	pharmaceutical	292:305	arg1	industries					307:316	food and pharmaceutical industries	283:316	food and pharmaceutical industries	283:316	Pectins are natural polysaccharides made from galacturonic acid residues, and they are widely used as an excipient in food and pharmaceutical industries.					
33497195	0	5	theme	Transfer	124:131	arg1	Spectrometry					151:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry.					
33497195	8	6	from	combination	1304:1314	arg1	time					1331:1334	real time	1326:1334	real time	1326:1334	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	7	7	theme	methyl	1240:1245	arg1	position					1253:1260	the methyl group position	1236:1260	the methyl group position along the galacturonic acid backbone	1236:1297	The Gal A isomers differed only in the methyl group position along the galacturonic acid backbone.					
33497195	3	8	theme	online	557:562	arg1	coupling					564:571	the successful online coupling	542:571	the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC)	542:687	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	7	9	theme	galacturonic	1272:1283	arg1	backbone					1290:1297	the galacturonic acid backbone	1268:1297	the galacturonic acid backbone	1268:1297	The Gal A isomers differed only in the methyl group position along the galacturonic acid backbone.					
33497195	3	10	theme	ultrahigh-performance	637:657	arg1	chromatography					666:679	ultrahigh-performance liquid chromatography	637:679	ultrahigh-performance liquid chromatography (UHPLC)	637:687	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	3	10	theme	ultrahigh-performance	637:657	arg1	UHPLC					682:686	UHPLC	682:686	UHPLC	682:686	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	3	11	theme	dissociation-mass	592:608	arg1	CTD-MS					624:629	charge transfer dissociation-mass spectrometry (CTD-MS)	576:630	charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC)	576:687	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	4	12	theme	data-dependent	828:841	arg1	mode					855:858	data-dependent acquisition mode	828:858	data-dependent acquisition mode	828:858	This work employed CTD fragmentation of the pectin mixtures in data-dependent acquisition mode.					
33497195	0	13	theme	Mass	146:149	arg1	Spectrometry					151:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry.					
33497195	3	14	theme	spectrometry	610:621	arg1	CTD-MS					624:629	charge transfer dissociation-mass spectrometry (CTD-MS)	576:630	charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC)	576:687	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	2	15	theme	methyl-esterification	333:353	arg1	solubility					509:518	the solubility	505:518	the solubility	505:518	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	15	theme	methyl-esterification	333:353	arg1	degree					323:328	The degree	319:328	The degree of methyl-esterification	319:353	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	15	theme	methyl-esterification	333:353	arg1	pattern					399:405	the linkage pattern	387:405	the linkage pattern	387:405	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	15	theme	methyl-esterification	333:353	arg1	composition					370:380	the monomeric composition	356:380	the monomeric composition	356:380	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	15	theme	methyl-esterification	333:353	arg1	factors					425:431	all important factors	411:431	all important factors	411:431	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	8	16	theme	complex	1406:1412	arg1	mixtures					1414:1421	complex mixtures	1406:1421	complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides	1406:1493	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	8	17	from	time	1331:1334	arg1	combination					1304:1314	The combination	1300:1314	The combination of CTD in real time with UHPLC	1300:1345	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	3	18	theme	charge	576:581	arg1	CTD-MS					624:629	charge transfer dissociation-mass spectrometry (CTD-MS)	576:630	charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC)	576:687	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	8	19	theme	new	1358:1360	arg1	tool					1362:1365	a new tool	1356:1365	a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides	1356:1493	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	2	20	theme	physical	452:459	arg1	properties					474:483	the physical and chemical properties	448:483	the physical and chemical properties of pectins	448:494	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	7	21	theme	group	1247:1251	arg1	position					1253:1260	the methyl group position	1236:1260	the methyl group position along the galacturonic acid backbone	1236:1297	The Gal A isomers differed only in the methyl group position along the galacturonic acid backbone.					
33497195	5	22	theme	informative	1017:1027	arg1	results					1029:1035	fewer ambiguous ions and more structurally informative results	974:1035	fewer ambiguous ions and more structurally informative results	974:1035	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	3	23	attach	derived	736:742	arg1	pectins					756:762	citrus pectins	749:762	citrus pectins	749:762	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	3	23	attach	derived	736:742	arg2	oligogalacturonans					717:734	oligogalacturonans	717:734	oligogalacturonans derived from citrus pectins	717:762	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	3	24	theme	transfer	583:590	arg1	CTD-MS					624:629	charge transfer dissociation-mass spectrometry (CTD-MS)	576:630	charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC)	576:687	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	5	25	theme	fewer	974:978	arg1	results					1029:1035	fewer ambiguous ions and more structurally informative results	974:1035	fewer ambiguous ions and more structurally informative results	974:1035	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	5	26	theme	dissociation	906:917	arg1	UHPLC-CID-MS					938:949	UHPLC-CID-MS	938:949	UHPLC-CID-MS	938:949	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	5	26	theme	dissociation	906:917	arg1	spectrometry					924:935	collision-induced dissociation mass spectrometry	888:935	collision-induced dissociation mass spectrometry (UHPLC-CID-MS)	888:950	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	6	27	theme	abundant	1084:1091	arg1	cleavages-and					1104:1116	abundant cross-ring cleavages-and	1084:1116	abundant cross-ring cleavages-and especially 1,4X, 1,5X, and 2,4X ions-which helped to identify most of the isomers	1084:1198	The developed UHPLC-CTD-MS method resulted in abundant cross-ring cleavages-and especially 1,4X, 1,5X, and 2,4X ions-which helped to identify most of the isomers.					
33497195	7	28	theme	Gal	1205:1207	arg1	isomers					1211:1217	The Gal A isomers	1201:1217	The Gal A isomers	1201:1217	The Gal A isomers differed only in the methyl group position along the galacturonic acid backbone.					
33497195	5	29	theme	ambiguous	980:988	arg1	ions					990:993	ambiguous ions	980:993	ambiguous ions	980:993	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	0	30	theme	Structural	0:9	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization of Isomeric Oligogalacturonan Mixtures	0:65	Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry.					
33497195	7	31	theme	A	1209:1209	arg1	isomers					1211:1217	The Gal A isomers	1201:1217	The Gal A isomers	1201:1217	The Gal A isomers differed only in the methyl group position along the galacturonic acid backbone.					
33497195	4	32	theme	mixtures	816:823	arg1	fragmentation					788:800	CTD fragmentation	784:800	CTD fragmentation of the pectin mixtures in data-dependent acquisition mode	784:858	This work employed CTD fragmentation of the pectin mixtures in data-dependent acquisition mode.					
33497195	0	33	theme	Isomeric	31:38	arg1	Mixtures					58:65	Isomeric Oligogalacturonan Mixtures	31:65	Isomeric Oligogalacturonan Mixtures	31:65	Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry.					
33497195	5	34	theme	mass	919:922	arg1	UHPLC-CID-MS					938:949	UHPLC-CID-MS	938:949	UHPLC-CID-MS	938:949	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	5	34	theme	mass	919:922	arg1	spectrometry					924:935	collision-induced dissociation mass spectrometry	888:935	collision-induced dissociation mass spectrometry (UHPLC-CID-MS)	888:950	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	5	35	dep	fewer	974:978	arg1	ions					990:993	ambiguous ions	980:993	ambiguous ions	980:993	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	2	36	theme	monomeric	360:368	arg1	solubility					509:518	the solubility	505:518	the solubility	505:518	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	36	theme	monomeric	360:368	arg1	degree					323:328	The degree	319:328	The degree of methyl-esterification	319:353	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	36	theme	monomeric	360:368	arg1	pattern					399:405	the linkage pattern	387:405	the linkage pattern	387:405	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	36	theme	monomeric	360:368	arg1	composition					370:380	the monomeric composition	356:380	the monomeric composition	356:380	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	36	theme	monomeric	360:368	arg1	factors					425:431	all important factors	411:431	all important factors	411:431	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	37	theme	pectins	488:494	arg1	properties					474:483	the physical and chemical properties	448:483	the physical and chemical properties of pectins	448:494	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	6	38	theme	cross-ring	1093:1102	arg1	cleavages-and					1104:1116	abundant cross-ring cleavages-and	1084:1116	abundant cross-ring cleavages-and especially 1,4X, 1,5X, and 2,4X ions-which helped to identify most of the isomers	1084:1198	The developed UHPLC-CTD-MS method resulted in abundant cross-ring cleavages-and especially 1,4X, 1,5X, and 2,4X ions-which helped to identify most of the isomers.					
33497195	8	39	theme	oligosaccharides	1478:1493	arg1	oligogalacturonans					1426:1443	oligogalacturonans	1426:1443	oligogalacturonans	1426:1443	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	8	39	theme	oligosaccharides	1478:1493	arg1	classes					1467:1473	potentially other classes	1449:1473	potentially other classes of oligosaccharides	1449:1493	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	3	40	theme	oligogalacturonans	717:734	arg1	isomers					706:712	isomers	706:712	isomers of oligogalacturonans derived from citrus pectins	706:762	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	3	41	theme	CTD-MS	624:629	arg1	coupling					564:571	the successful online coupling	542:571	the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC)	542:687	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	0	42	theme	Mixtures	58:65	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization of Isomeric Oligogalacturonan Mixtures	0:65	Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry.					
33497195	2	43	theme	linkage	391:397	arg1	solubility					509:518	the solubility	505:518	the solubility	505:518	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	43	theme	linkage	391:397	arg1	degree					323:328	The degree	319:328	The degree of methyl-esterification	319:353	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	43	theme	linkage	391:397	arg1	pattern					399:405	the linkage pattern	387:405	the linkage pattern	387:405	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	43	theme	linkage	391:397	arg1	composition					370:380	the monomeric composition	356:380	the monomeric composition	356:380	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	43	theme	linkage	391:397	arg1	factors					425:431	all important factors	411:431	all important factors	411:431	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	3	44	with	CTD-MS	624:629	arg1	chromatography					666:679	ultrahigh-performance liquid chromatography	637:679	ultrahigh-performance liquid chromatography (UHPLC)	637:687	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	3	44	with	CTD-MS	624:629	arg1	UHPLC					682:686	UHPLC	682:686	UHPLC	682:686	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	2	45	theme	chemical	465:472	arg1	properties					474:483	the physical and chemical properties	448:483	the physical and chemical properties of pectins	448:494	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	0	46	theme	Oligogalacturonan	40:56	arg1	Mixtures					58:65	Isomeric Oligogalacturonan Mixtures	31:65	Isomeric Oligogalacturonan Mixtures	31:65	Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry.					
33497195	8	47	theme	structural	1375:1384	arg1	characterization					1386:1401	the structural characterization	1371:1401	the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides	1371:1493	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	6	48	theme	2,4X	1145:1148	arg1	ions-which					1150:1159	2,4X ions-which	1145:1159	2,4X ions-which	1145:1159	The developed UHPLC-CTD-MS method resulted in abundant cross-ring cleavages-and especially 1,4X, 1,5X, and 2,4X ions-which helped to identify most of the isomers.					
33497195	0	49	theme	Ultrahigh-Performance	73:93	arg1	Spectrometry					151:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry.					
33497195	3	50	theme	successful	546:555	arg1	coupling					564:571	the successful online coupling	542:571	the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC)	542:687	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	8	51	theme	real	1326:1329	arg1	time					1331:1334	real time	1326:1334	real time	1326:1334	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	1	52	used	used	259:262	arg2	excipient					270:278	an excipient	267:278	an excipient in food and pharmaceutical industries	267:316	Pectins are natural polysaccharides made from galacturonic acid residues, and they are widely used as an excipient in food and pharmaceutical industries.					
33497195	1	52	used	used	259:262	arg2	they					243:246	they	243:246	they	243:246	Pectins are natural polysaccharides made from galacturonic acid residues, and they are widely used as an excipient in food and pharmaceutical industries.					
33497195	5	53	theme	collision-induced	888:904	arg1	UHPLC-CID-MS					938:949	UHPLC-CID-MS	938:949	UHPLC-CID-MS	938:949	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	5	53	theme	collision-induced	888:904	arg1	spectrometry					924:935	collision-induced dissociation mass spectrometry	888:935	collision-induced dissociation mass spectrometry (UHPLC-CID-MS)	888:950	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	8	54	from	CTD	1319:1321	arg1	time					1331:1334	real time	1326:1334	real time	1326:1334	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	3	55	theme	liquid	659:664	arg1	chromatography					666:679	ultrahigh-performance liquid chromatography	637:679	ultrahigh-performance liquid chromatography (UHPLC)	637:687	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	3	55	theme	liquid	659:664	arg1	UHPLC					682:686	UHPLC	682:686	UHPLC	682:686	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	8	56	with	combination	1304:1314	arg1	UHPLC					1341:1345	UHPLC	1341:1345	UHPLC	1341:1345	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	1	57	theme	natural	177:183	arg1	polysaccharides					185:199	natural polysaccharides	177:199	natural polysaccharides made from galacturonic acid residues	177:236	Pectins are natural polysaccharides made from galacturonic acid residues, and they are widely used as an excipient in food and pharmaceutical industries.					
33497195	1	57	theme	natural	177:183	arg1	Pectins					165:171	Pectins	165:171	Pectins	165:171	Pectins are natural polysaccharides made from galacturonic acid residues, and they are widely used as an excipient in food and pharmaceutical industries.					
33497195	6	58	theme	UHPLC-CTD-MS	1052:1063	arg1	method					1065:1070	The developed UHPLC-CTD-MS method	1038:1070	The developed UHPLC-CTD-MS method	1038:1070	The developed UHPLC-CTD-MS method resulted in abundant cross-ring cleavages-and especially 1,4X, 1,5X, and 2,4X ions-which helped to identify most of the isomers.					
33497195	1	59	from	excipient	270:278	arg1	industries					307:316	food and pharmaceutical industries	283:316	food and pharmaceutical industries	283:316	Pectins are natural polysaccharides made from galacturonic acid residues, and they are widely used as an excipient in food and pharmaceutical industries.					
33497195	4	60	theme	pectin	809:814	arg1	mixtures					816:823	the pectin mixtures	805:823	the pectin mixtures	805:823	This work employed CTD fragmentation of the pectin mixtures in data-dependent acquisition mode.					
33497195	4	61	theme	acquisition	843:853	arg1	mode					855:858	data-dependent acquisition mode	828:858	data-dependent acquisition mode	828:858	This work employed CTD fragmentation of the pectin mixtures in data-dependent acquisition mode.					
33497195	5	62	with	UHPLC	877:881	arg1	UHPLC-CID-MS					938:949	UHPLC-CID-MS	938:949	UHPLC-CID-MS	938:949	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	5	62	with	UHPLC	877:881	arg1	spectrometry					924:935	collision-induced dissociation mass spectrometry	888:935	collision-induced dissociation mass spectrometry (UHPLC-CID-MS)	888:950	Compared to the UHPLC with collision-induced dissociation mass spectrometry (UHPLC-CID-MS), UHPLC-CTD-MS yielded fewer ambiguous ions and more structurally informative results.					
33497195	8	63	theme	classes	1467:1473	arg1	mixtures					1414:1421	complex mixtures	1406:1421	complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides	1406:1493	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	1	64	theme	galacturonic	211:222	arg1	residues					229:236	galacturonic acid residues	211:236	galacturonic acid residues	211:236	Pectins are natural polysaccharides made from galacturonic acid residues, and they are widely used as an excipient in food and pharmaceutical industries.					
33497195	8	65	theme	other	1461:1465	arg1	classes					1467:1473	potentially other classes	1449:1473	potentially other classes of oligosaccharides	1449:1493	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	8	66	theme	mixtures	1414:1421	arg1	characterization					1386:1401	the structural characterization	1371:1401	the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides	1371:1493	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	0	67	theme	Chromatography-Charge	102:122	arg1	Spectrometry					151:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry.					
33497195	3	68	theme	citrus	749:754	arg1	pectins					756:762	citrus pectins	749:762	citrus pectins	749:762	This work focuses on the successful online coupling of charge transfer dissociation-mass spectrometry (CTD-MS) with ultrahigh-performance liquid chromatography (UHPLC) to differentiate isomers of oligogalacturonans derived from citrus pectins.					
33497195	8	69	theme	oligogalacturonans	1426:1443	arg1	mixtures					1414:1421	complex mixtures	1406:1421	complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides	1406:1493	The combination of CTD in real time with UHPLC provides a new tool for the structural characterization of complex mixtures of oligogalacturonans and potentially other classes of oligosaccharides.					
33497195	4	70	from	fragmentation	788:800	arg1	mode					855:858	data-dependent acquisition mode	828:858	data-dependent acquisition mode	828:858	This work employed CTD fragmentation of the pectin mixtures in data-dependent acquisition mode.					
33497195	1	71	theme	food	283:286	arg1	industries					307:316	food and pharmaceutical industries	283:316	food and pharmaceutical industries	283:316	Pectins are natural polysaccharides made from galacturonic acid residues, and they are widely used as an excipient in food and pharmaceutical industries.					
33497195	0	72	theme	Liquid	95:100	arg1	Spectrometry					151:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry	73:162	Structural Characterization of Isomeric Oligogalacturonan Mixtures Using Ultrahigh-Performance Liquid Chromatography-Charge Transfer Dissociation Mass Spectrometry.					
33497195	6	73	theme	developed	1042:1050	arg1	method					1065:1070	The developed UHPLC-CTD-MS method	1038:1070	The developed UHPLC-CTD-MS method	1038:1070	The developed UHPLC-CTD-MS method resulted in abundant cross-ring cleavages-and especially 1,4X, 1,5X, and 2,4X ions-which helped to identify most of the isomers.					
33497195	7	74	theme	acid	1285:1288	arg1	backbone					1290:1297	the galacturonic acid backbone	1268:1297	the galacturonic acid backbone	1268:1297	The Gal A isomers differed only in the methyl group position along the galacturonic acid backbone.					
33497195	2	75	theme	important	415:423	arg1	solubility					509:518	the solubility	505:518	the solubility	505:518	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	75	theme	important	415:423	arg1	degree					323:328	The degree	319:328	The degree of methyl-esterification	319:353	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	75	theme	important	415:423	arg1	pattern					399:405	the linkage pattern	387:405	the linkage pattern	387:405	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	75	theme	important	415:423	arg1	composition					370:380	the monomeric composition	356:380	the monomeric composition	356:380	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
33497195	2	75	theme	important	415:423	arg1	factors					425:431	all important factors	411:431	all important factors	411:431	The degree of methyl-esterification, the monomeric composition, and the linkage pattern are all important factors that influence the physical and chemical properties of pectins, such as the solubility.					
34029582	1	0	dep	polysaccharides	266:280	arg1	BPs					283:285	BPs	283:285	BPs: Hw, Ac, Al and Na	283:304	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	6	1	theme	molecular	1017:1025	arg1	weight					1027:1032	the higher molecular weight	1006:1032	the higher molecular weight of blackberry polysaccharide	1006:1061	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	6	1	theme	molecular	1017:1025	arg1	ingredient					1108:1117	a beneficial bioactive ingredient	1085:1117	a beneficial bioactive ingredient for diabetes mellitus and complications	1085:1157	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	1	2	theme	NaCl	202:205	arg1	solution					207:214	0.1 M NaOH and 0.1 M NaCl solution	181:214	solution	207:214	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	0	3	theme	physicochemical	61:75	arg1	properties					77:86	physicochemical properties	61:86	physicochemical properties	61:86	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	0	4	from	study	12:16	arg1	effect					25:30	the effect	21:30	the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides	21:138	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	1	5	theme	hot	156:158	arg1	water					160:164	hot water	156:164	hot water	156:164	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	4	6	theme	higher	724:729	arg1	weight					741:746	higher molecular weight	724:746	higher molecular weight	724:746	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	5	7	theme	acid	925:928	arg1	weight					883:888	higher molecular weight	866:888	higher molecular weight	866:888	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	5	7	theme	acid	925:928	arg1	content					901:907	higher content	894:907	higher content of galacturonic acid	894:928	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	3	8	theme	triple-helical	505:518	arg1	structure					520:528	triple-helical structure	505:528	triple-helical structure	505:528	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	5	9	theme	better	940:945	arg1	inhibition					947:956	better inhibition	940:956	better inhibition of AGEs formation	940:974	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	3	10	theme	particle	490:497	arg1	size					499:502	particle size	490:502	particle size	490:502	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	5	11	with	BPs	857:859	arg1	weight					883:888	higher molecular weight	866:888	higher molecular weight	866:888	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	5	11	with	BPs	857:859	arg1	content					901:907	higher content	894:907	higher content of galacturonic acid	894:928	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	6	12	theme	bioactive	1098:1106	arg1	weight					1027:1032	the higher molecular weight	1006:1032	the higher molecular weight of blackberry polysaccharide	1006:1061	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	6	12	theme	bioactive	1098:1106	arg1	ingredient					1108:1117	a beneficial bioactive ingredient	1085:1117	a beneficial bioactive ingredient for diabetes mellitus and complications	1085:1157	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	0	13	theme	blackberry	107:116	arg1	polysaccharides					124:138	blackberry fruit polysaccharides	107:138	blackberry fruit polysaccharides	107:138	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	1	14	dep	BPs	283:285	arg1	Al					296:297	Al	296:297	Al	296:297	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	1	14	dep	BPs	283:285	arg1	Hw					288:289	Hw	288:289	Hw	288:289	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	1	14	dep	BPs	283:285	arg1	Na					303:304	Na	303:304	Na	303:304	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	3	15	theme	monosaccharide	462:475	arg1	composition					477:487	monosaccharide composition	462:487	monosaccharide composition	462:487	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	4	16	theme	Bioactivity	630:640	arg1	assays					642:647	Bioactivity assays	630:647	Bioactivity assays	630:647	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	2	17	theme	physicochemical	312:326	arg1	properties					328:337	The physicochemical properties	308:337	The physicochemical properties	308:337	The physicochemical properties and biological activities were then investigated and compared.					
34029582	5	18	theme	higher	866:871	arg1	weight					883:888	higher molecular weight	866:888	higher molecular weight	866:888	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	4	19	theme	inhibitory	791:800	arg1	activity					802:809	stronger antioxidant and α-glucosidase inhibitory activity	752:809	activity	802:809	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	5	20	theme	anti-glycated	822:834	arg1	assay					836:840	anti-glycated assay	822:840	anti-glycated assay	822:840	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	5	21	theme	formation	966:974	arg1	inhibition					947:956	better inhibition	940:956	better inhibition of AGEs formation	940:974	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	1	22	used	used	232:235	arg2	water					160:164	hot water	156:164	hot water	156:164	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	1	22	used	used	232:235	arg2	HCl					173:175	0.1 M HCl	167:175	0.1 M HCl	167:175	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	1	22	used	used	232:235	arg2	NaOH					187:190	0.1 M NaOH and 0.1 M NaCl solution	181:214	NaOH	187:190	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	0	23	dep	properties	77:86	arg1	the					57:59	the	57:59	the	57:59	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	5	24	contain	possessed	930:938	arg2	inhibition					947:956	better inhibition	940:956	better inhibition of AGEs formation	940:974	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	5	24	contain	possessed	930:938	arg1	BPs					857:859	BPs	857:859	BPs with higher molecular weight and higher content of galacturonic acid	857:928	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	1	25	theme	0.1	167:169	arg1	M					171:171	M	171:171	M	171:171	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	0	26	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.	0:139	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	1	27	theme	M	171:171	arg1	HCl					173:175	0.1 M HCl	167:175	0.1 M HCl	167:175	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	0	28	theme	polysaccharides	124:138	arg1	bioactivity					92:102	bioactivity	92:102	bioactivity	92:102	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	0	28	theme	polysaccharides	124:138	arg1	properties					77:86	physicochemical properties	61:86	physicochemical properties	61:86	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	5	29	theme	molecular	873:881	arg1	weight					883:888	higher molecular weight	866:888	higher molecular weight	866:888	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	5	30	theme	galacturonic	912:923	arg1	acid					925:928	galacturonic acid	912:928	galacturonic acid	912:928	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	0	31	theme	fruit	118:122	arg1	polysaccharides					124:138	blackberry fruit polysaccharides	107:138	blackberry fruit polysaccharides	107:138	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	3	32	theme	molecular	444:452	arg1	weight					454:459	molecular weight	444:459	molecular weight	444:459	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	2	33	theme	biological	343:352	arg1	activities					354:363	biological activities	343:363	biological activities	343:363	The physicochemical properties and biological activities were then investigated and compared.					
34029582	1	34	dep	Hw	288:289	arg1	Ac					292:293	Ac	292:293	Ac	292:293	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	4	35	dep	antioxidant	761:771	arg1	stronger					752:759	stronger	752:759	stronger	752:759	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	6	36	theme	blackberry	1037:1046	arg1	polysaccharide					1048:1061	blackberry polysaccharide	1037:1061	blackberry polysaccharide	1037:1061	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	1	37	theme	0.1	181:183	arg1	M					185:185	M	185:185	M	185:185	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	6	38	theme	polysaccharide	1048:1061	arg1	weight					1027:1032	the higher molecular weight	1006:1032	the higher molecular weight of blackberry polysaccharide	1006:1061	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	6	38	theme	polysaccharide	1048:1061	arg1	ingredient					1108:1117	a beneficial bioactive ingredient	1085:1117	a beneficial bioactive ingredient for diabetes mellitus and complications	1085:1157	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	1	39	theme	blackberry	255:264	arg1	polysaccharides					266:280	blackberry polysaccharides	255:280	blackberry polysaccharides (BPs: Hw, Ac, Al and Na)	255:305	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	1	40	theme	M	185:185	arg1	NaOH					187:190	0.1 M NaOH and 0.1 M NaCl solution	181:214	NaOH	187:190	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	0	41	theme	solvent	46:52	arg1	effect					25:30	the effect	21:30	the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides	21:138	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	1	42	theme	polysaccharides	266:280	arg1	extraction					241:250	extraction	241:250	extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na)	241:305	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	4	43	theme	α-glucosidase	777:789	arg1	activity					802:809	stronger antioxidant and α-glucosidase inhibitory activity	752:809	activity	802:809	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	3	44	theme	extraction	426:435	arg1	yield					437:441	the extraction yield	422:441	the extraction yield	422:441	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	0	45	theme	extraction	35:44	arg1	solvent					46:52	extraction solvent	35:52	extraction solvent	35:52	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	4	46	with	polysaccharides	691:705	arg1	weight					741:746	higher molecular weight	724:746	higher molecular weight	724:746	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	3	47	theme	surface	531:537	arg1	morphology					539:548	surface morphology	531:548	surface morphology	531:548	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	0	48	from	effect	25:30	arg1	bioactivity					92:102	bioactivity	92:102	bioactivity	92:102	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	0	48	from	effect	25:30	arg1	properties					77:86	physicochemical properties	61:86	physicochemical properties	61:86	Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides.					
34029582	4	49	theme	molecular	731:739	arg1	weight					741:746	higher molecular weight	724:746	higher molecular weight	724:746	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	4	50	dep	polysaccharides	691:705	arg1	Na					715:716	Na	715:716	Na	715:716	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	4	50	dep	polysaccharides	691:705	arg1	Hw					708:709	Hw	708:709	Hw	708:709	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	4	50	dep	polysaccharides	691:705	arg1	polysaccharides					691:705	the polysaccharides	687:705	the polysaccharides (Hw and Na) with higher molecular weight	687:746	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	3	51	theme	extraction	609:618	arg1	solvents					620:627	extraction solvents	609:627	extraction solvents	609:627	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	3	52	theme	BPs	580:582	arg1	morphology					539:548	surface morphology	531:548	surface morphology	531:548	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	3	52	theme	BPs	580:582	arg1	weight					454:459	molecular weight	444:459	molecular weight	444:459	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	3	52	theme	BPs	580:582	arg1	size					499:502	particle size	490:502	particle size	490:502	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	3	52	theme	BPs	580:582	arg1	structure					520:528	triple-helical structure	505:528	triple-helical structure	505:528	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	3	52	theme	BPs	580:582	arg1	properties					566:575	rheological properties	554:575	rheological properties	554:575	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	3	52	theme	BPs	580:582	arg1	yield					437:441	the extraction yield	422:441	the extraction yield	422:441	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	3	52	theme	BPs	580:582	arg1	composition					477:487	monosaccharide composition	462:487	monosaccharide composition	462:487	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	5	53	theme	higher	894:899	arg1	content					901:907	higher content	894:907	higher content of galacturonic acid	894:928	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	6	54	theme	diabetes	1123:1130	arg1	mellitus					1132:1139	diabetes mellitus	1123:1139	diabetes mellitus	1123:1139	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	4	55	contain	had	748:750	arg1	Na					715:716	Na	715:716	Na	715:716	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	4	55	contain	had	748:750	arg2	antioxidant					761:771	antioxidant	761:771	antioxidant	761:771	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	4	55	contain	had	748:750	arg1	Hw					708:709	Hw	708:709	Hw	708:709	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	4	55	contain	had	748:750	arg1	polysaccharides					691:705	the polysaccharides	687:705	the polysaccharides (Hw and Na) with higher molecular weight	687:746	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	4	55	contain	had	748:750	arg2	activity					802:809	stronger antioxidant and α-glucosidase inhibitory activity	752:809	activity	802:809	Bioactivity assays implied that the four BPs showed that the polysaccharides (Hw and Na) with higher molecular weight had stronger antioxidant and α-glucosidase inhibitory activity.					
34029582	6	56	theme	higher	1010:1015	arg1	weight					1027:1032	the higher molecular weight	1006:1032	the higher molecular weight of blackberry polysaccharide	1006:1061	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	6	56	theme	higher	1010:1015	arg1	ingredient					1108:1117	a beneficial bioactive ingredient	1085:1117	a beneficial bioactive ingredient for diabetes mellitus and complications	1085:1157	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	3	57	theme	rheological	554:564	arg1	properties					566:575	rheological properties	554:575	rheological properties	554:575	Results showed that the extraction yield, molecular weight, monosaccharide composition, particle size, triple-helical structure, surface morphology and rheological properties of BPs were greatly affected by extraction solvents.					
34029582	6	58	theme	beneficial	1087:1096	arg1	weight					1027:1032	the higher molecular weight	1006:1032	the higher molecular weight of blackberry polysaccharide	1006:1061	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	6	58	theme	beneficial	1087:1096	arg1	ingredient					1108:1117	a beneficial bioactive ingredient	1085:1117	a beneficial bioactive ingredient for diabetes mellitus and complications	1085:1157	These results suggested that the higher molecular weight of blackberry polysaccharide could be developed as a beneficial bioactive ingredient for diabetes mellitus and complications.					
34029582	1	59	theme	0.1	196:198	arg1	M					200:200	M	200:200	M	200:200	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
34029582	5	60	theme	AGEs	961:964	arg1	formation					966:974	AGEs formation	961:974	AGEs formation	961:974	Moreover, anti-glycated assay indicated that BPs with higher molecular weight and higher content of galacturonic acid possessed better inhibition of AGEs formation.					
34029582	1	61	theme	M	200:200	arg1	solution					207:214	0.1 M NaOH and 0.1 M NaCl solution	181:214	solution	207:214	In this study, hot water, 0.1 M HCl and 0.1 M NaOH and 0.1 M NaCl solution were separately used for extraction of blackberry polysaccharides (BPs: Hw, Ac, Al and Na).					
33736278	1	0	theme	Coprinus	147:154	arg1	comatus					156:162	Coprinus comatus	147:162	Coprinus comatus	147:162	Many effects of Coprinus comatus are attributed to its polysaccharide components.					
33736278	4	1	from	influences	697:706	arg1	proliferation					742:754	the proliferation	738:754	the proliferation of lactobacillus and Bifidobacterium in vitro	738:800	At the same time, the influences of the two polysaccharides on the proliferation of lactobacillus and Bifidobacterium in vitro were compared.					
33736278	0	2	theme	polysaccharides	68:82	arg1	structures					18:27	structures	18:27	structures	18:27	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	0	2	theme	polysaccharides	68:82	arg1	effects					48:54	prebiotic-like effects	33:54	prebiotic-like effects in vitro	33:63	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	7	3	dep	group	1177:1181	arg1	group					1192:1196	group	1192:1196	group	1192:1196	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	1	4	theme	comatus	156:162	arg1	effects					136:142	Many effects	131:142	Many effects of Coprinus comatus	131:162	Many effects of Coprinus comatus are attributed to its polysaccharide components.					
33736278	3	5	theme	prepared	634:641	arg1	polysaccharides					643:657	the two prepared polysaccharides	626:657	the two prepared polysaccharides	626:657	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	7	6	from	effects	1008:1014	arg1	proliferation					1059:1071	the proliferation	1055:1071	the proliferation of the selected probiotics	1055:1098	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	3	7	theme	molecular	543:551	arg1	weights					553:559	molecular weights	543:559	molecular weights	543:559	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	0	8	theme	Coprinus	89:96	arg1	body					112:115	Coprinus comatus fruit body	89:115	Coprinus comatus fruit body	89:115	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	7	9	theme	prepared	1027:1034	arg1	polysaccharides					1036:1050	the two prepared polysaccharides	1019:1050	the two prepared polysaccharides	1019:1050	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	3	10	theme	carbohydrate	491:502	arg1	contents					504:511	The total carbohydrate contents	481:511	The total carbohydrate contents	481:511	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	4	11	theme	polysaccharides	719:733	arg1	influences					697:706	the influences	693:706	the influences of the two polysaccharides on the proliferation of lactobacillus and Bifidobacterium in vitro	693:800	At the same time, the influences of the two polysaccharides on the proliferation of lactobacillus and Bifidobacterium in vitro were compared.					
33736278	0	12	from	effects	48:54	arg1	mycelium					121:128	mycelium	121:128	mycelium	121:128	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	0	12	from	effects	48:54	arg1	body					112:115	Coprinus comatus fruit body	89:115	Coprinus comatus fruit body	89:115	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	2	13	theme	Coprinus	259:266	arg1	polysaccharides					276:290	Coprinus comatus polysaccharides	259:290	Coprinus comatus polysaccharides as the research topic	259:312	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	2	14	theme	bodies	398:403	arg1	polysaccharides					434:448	the intracellular polysaccharides	416:448	the intracellular polysaccharides of liquid fermentation (ICPs)	416:478	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	2	14	theme	bodies	398:403	arg1	polysaccharides					353:367	the polysaccharides	349:367	the polysaccharides of Coprinus comatus fruiting bodies (CBPs)	349:410	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	0	15	theme	fruit	106:110	arg1	body					112:115	Coprinus comatus fruit body	89:115	Coprinus comatus fruit body	89:115	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	0	16	dep	Coprinus	89:96	arg1	comatus					98:104	comatus	98:104	comatus	98:104	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	2	17	theme	fruiting	389:396	arg1	CBPs					406:409	CBPs	406:409	CBPs	406:409	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	2	17	theme	fruiting	389:396	arg1	bodies					398:403	Coprinus comatus fruiting bodies	372:403	Coprinus comatus fruiting bodies (CBPs)	372:410	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	5	18	theme	structural	821:830	arg1	analysis					832:839	The structural analysis	817:839	The structural analysis	817:839	The structural analysis exhibited that there were slight differences in the two prepared polysaccharides.					
33736278	7	19	theme	dose-dependent	1105:1118	arg1	manners					1120:1126	dose-dependent manners	1105:1126	dose-dependent manners	1105:1126	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	7	19	theme	dose-dependent	1105:1118	arg1	effects					1008:1014	the effects	1004:1014	the effects of the two prepared polysaccharides on the proliferation of the selected probiotics	1004:1098	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	2	20	theme	research	299:306	arg1	topic					308:312	the research topic	295:312	the research topic	295:312	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	4	21	theme	Bifidobacterium	777:791	arg1	proliferation					742:754	the proliferation	738:754	the proliferation of lactobacillus and Bifidobacterium in vitro	738:800	At the same time, the influences of the two polysaccharides on the proliferation of lactobacillus and Bifidobacterium in vitro were compared.					
33736278	2	22	theme	Coprinus	372:379	arg1	CBPs					406:409	CBPs	406:409	CBPs	406:409	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	2	22	theme	Coprinus	372:379	arg1	bodies					398:403	Coprinus comatus fruiting bodies	372:403	Coprinus comatus fruiting bodies (CBPs)	372:410	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	0	23	theme	structures	18:27	arg1	Comparison					0:9	Comparison	0:9	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.	0:129	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	7	24	theme	selected	1080:1087	arg1	probiotics					1089:1098	the selected probiotics	1076:1098	the selected probiotics	1076:1098	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	4	25	theme	lactobacillus	759:771	arg1	proliferation					742:754	the proliferation	738:754	the proliferation of lactobacillus and Bifidobacterium in vitro	738:800	At the same time, the influences of the two polysaccharides on the proliferation of lactobacillus and Bifidobacterium in vitro were compared.					
33736278	1	26	theme	polysaccharide	186:199	arg1	components					201:210	its polysaccharide components	182:210	its polysaccharide components	182:210	Many effects of Coprinus comatus are attributed to its polysaccharide components.					
33736278	7	27	theme	probiotics	1089:1098	arg1	proliferation					1059:1071	the proliferation	1055:1071	the proliferation of the selected probiotics	1055:1098	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	3	28	theme	functional	562:571	arg1	polysaccharides					643:657	the two prepared polysaccharides	626:657	the two prepared polysaccharides	626:657	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	3	28	theme	functional	562:571	arg1	groups					573:578	functional groups	562:578	functional groups	562:578	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	0	29	theme	prebiotic-like	33:46	arg1	effects					48:54	prebiotic-like effects	33:54	prebiotic-like effects in vitro	33:63	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	6	30	used	utilized	960:967	arg2	CBPs					946:949	CBPs	946:949	CBPs	946:949	However, both ICPs and CBPs could be utilized by these two strains.					
33736278	6	30	used	utilized	960:967	arg2	ICPs					937:940	ICPs	937:940	ICPs	937:940	However, both ICPs and CBPs could be utilized by these two strains.					
33736278	3	31	theme	functional	601:610	arg1	properties					612:621	functional properties	601:621	functional properties	601:621	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	4	32	theme	same	682:685	arg1	time					687:690	the same time	678:690	the same time	678:690	At the same time, the influences of the two polysaccharides on the proliferation of lactobacillus and Bifidobacterium in vitro were compared.					
33736278	5	33	theme	slight	867:872	arg1	differences					874:884	slight differences	867:884	slight differences in the two prepared polysaccharides	867:920	The structural analysis exhibited that there were slight differences in the two prepared polysaccharides.					
33736278	3	34	theme	polysaccharides	643:657	arg1	contents					504:511	The total carbohydrate contents	481:511	The total carbohydrate contents	481:511	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	3	34	theme	polysaccharides	643:657	arg1	polysaccharides					643:657	the two prepared polysaccharides	626:657	the two prepared polysaccharides	626:657	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	3	34	theme	polysaccharides	643:657	arg1	properties					612:621	functional properties	601:621	functional properties	601:621	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	3	34	theme	polysaccharides	643:657	arg1	groups					573:578	functional groups	562:578	functional groups	562:578	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	3	34	theme	polysaccharides	643:657	arg1	compositions					529:540	monosaccharide compositions	514:540	monosaccharide compositions	514:540	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	3	34	theme	polysaccharides	643:657	arg1	microstructures					581:595	microstructures	581:595	microstructures	581:595	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	3	34	theme	polysaccharides	643:657	arg1	weights					553:559	molecular weights	543:559	molecular weights	543:559	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	2	35	theme	intracellular	420:432	arg1	polysaccharides					434:448	the intracellular polysaccharides	416:448	the intracellular polysaccharides of liquid fermentation (ICPs)	416:478	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	7	36	contain	had	1198:1200	arg1	group					1177:1181	the ICPs group	1168:1181	the ICPs group	1168:1181	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	7	36	contain	had	1198:1200	arg2	P > 0.05					1229:1236	P > 0.05	1229:1236	P > 0.05	1229:1236	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	7	36	contain	had	1198:1200	arg2	difference					1217:1226	no significant difference	1202:1226	no significant difference (P > 0.05)	1202:1237	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	7	36	contain	had	1198:1200	arg1	CBPs					1187:1190	CBPs	1187:1190	CBPs	1187:1190	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	2	37	theme	fermentation	460:471	arg1	polysaccharides					434:448	the intracellular polysaccharides	416:448	the intracellular polysaccharides of liquid fermentation (ICPs)	416:478	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	2	37	theme	fermentation	460:471	arg1	polysaccharides					353:367	the polysaccharides	349:367	the polysaccharides of Coprinus comatus fruiting bodies (CBPs)	349:410	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	5	38	from	differences	874:884	arg1	polysaccharides					906:920	the two prepared polysaccharides	889:920	the two prepared polysaccharides	889:920	The structural analysis exhibited that there were slight differences in the two prepared polysaccharides.					
33736278	0	39	theme	effects	48:54	arg1	Comparison					0:9	Comparison	0:9	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.	0:129	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	0	40	from	body	112:115	arg1	polysaccharides					68:82	polysaccharides	68:82	polysaccharides from Coprinus comatus fruit body and mycelium	68:128	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	0	40	from	body	112:115	arg1	structures					18:27	structures	18:27	structures	18:27	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	0	40	from	body	112:115	arg1	effects					48:54	prebiotic-like effects	33:54	prebiotic-like effects in vitro	33:63	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	3	41	theme	total	485:489	arg1	contents					504:511	The total carbohydrate contents	481:511	The total carbohydrate contents	481:511	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	7	42	theme	ICPs	1172:1175	arg1	group					1177:1181	the ICPs group	1168:1181	the ICPs group	1168:1181	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	7	43	theme	polysaccharides	1036:1050	arg1	manners					1120:1126	dose-dependent manners	1105:1126	dose-dependent manners	1105:1126	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	7	43	theme	polysaccharides	1036:1050	arg1	effects					1008:1014	the effects	1004:1014	the effects of the two prepared polysaccharides on the proliferation of the selected probiotics	1004:1098	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	0	44	dep	structures	18:27	arg1	the					14:16	the	14:16	the	14:16	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	8	45	theme	equivalent	1296:1305	arg1	replacer					1307:1314	an equivalent replacer	1293:1314	an equivalent replacer for CBPs	1293:1323	Therefore, this work demonstrates that ICPs could be an equivalent replacer for CBPs.					
33736278	8	45	theme	equivalent	1296:1305	arg1	ICPs					1279:1282	ICPs	1279:1282	ICPs	1279:1282	Therefore, this work demonstrates that ICPs could be an equivalent replacer for CBPs.					
33736278	5	46	theme	prepared	897:904	arg1	polysaccharides					906:920	the two prepared polysaccharides	889:920	the two prepared polysaccharides	889:920	The structural analysis exhibited that there were slight differences in the two prepared polysaccharides.					
33736278	2	47	dep	Coprinus	372:379	arg1	comatus					381:387	comatus	381:387	comatus	381:387	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	1	48	theme	Many	131:134	arg1	effects					136:142	Many effects	131:142	Many effects of Coprinus comatus	131:162	Many effects of Coprinus comatus are attributed to its polysaccharide components.					
33736278	2	49	theme	liquid	453:458	arg1	fermentation					460:471	liquid fermentation	453:471	liquid fermentation (ICPs)	453:478	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	2	49	theme	liquid	453:458	arg1	ICPs					474:477	ICPs	474:477	ICPs	474:477	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	2	50	theme	article	240:246	arg1	aim					228:230	the aim	224:230	the aim of this article	224:246	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	2	51	dep	Coprinus	259:266	arg1	comatus					268:274	comatus	268:274	comatus	268:274	Therefore, the aim of this article is to take Coprinus comatus polysaccharides as the research topic to estimate the difference between the polysaccharides of Coprinus comatus fruiting bodies (CBPs) and the intracellular polysaccharides of liquid fermentation (ICPs).					
33736278	0	52	from	mycelium	121:128	arg1	polysaccharides					68:82	polysaccharides	68:82	polysaccharides from Coprinus comatus fruit body and mycelium	68:128	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	0	52	from	mycelium	121:128	arg1	structures					18:27	structures	18:27	structures	18:27	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	0	52	from	mycelium	121:128	arg1	effects					48:54	prebiotic-like effects	33:54	prebiotic-like effects in vitro	33:63	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	7	53	theme	experiment	1152:1161	arg1	scope					1139:1143	the scope	1135:1143	the scope of the experiment	1135:1161	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	0	54	from	structures	18:27	arg1	mycelium					121:128	mycelium	121:128	mycelium	121:128	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	0	54	from	structures	18:27	arg1	body					112:115	Coprinus comatus fruit body	89:115	Coprinus comatus fruit body	89:115	Comparison of the structures and prebiotic-like effects in vitro of polysaccharides from Coprinus comatus fruit body and mycelium.					
33736278	3	55	theme	monosaccharide	514:527	arg1	compositions					529:540	monosaccharide compositions	514:540	monosaccharide compositions	514:540	The total carbohydrate contents, monosaccharide compositions, molecular weights, functional groups, microstructures and functional properties of the two prepared polysaccharides were evaluated.					
33736278	7	56	theme	significant	1205:1215	arg1	P > 0.05					1229:1236	P > 0.05	1229:1236	P > 0.05	1229:1236	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
33736278	7	56	theme	significant	1205:1215	arg1	difference					1217:1226	no significant difference	1202:1226	no significant difference (P > 0.05)	1202:1237	Furthermore, the effects of the two prepared polysaccharides on the proliferation of the selected probiotics were dose-dependent manners within the scope of the experiment, and the ICPs group and CBPs group had no significant difference (P > 0.05).					
32439445	5	0	theme	composite	687:695	arg1	hydrogels					697:705	the composite hydrogels	683:705	the composite hydrogels	683:705	Frequency sweeps indicated that remarkable mechanical properties were exhibited by the composite hydrogels.					
32439445	1	1	theme	cosmetic	156:163	arg1	industry					165:172	biomedical and cosmetic industry	141:172	biomedical and cosmetic industry	141:172	Shear thinning hydrogels are majorly employed in biomedical and cosmetic industry.					
32439445	4	2	dep	thinning	547:554	arg1	the					537:539	the	537:539	the	537:539	Comprehensive rheological analysis was conducted to understand the shear thinning and self-healing behaviour of the hydrogel.					
32439445	7	3	theme	hydrogels	923:931	arg1	establishment					862:874	establishment	862:874	establishment of natural polysaccharide-based shear thinning hydrogels for possible application in various sectors	862:975	This study can, therefore, act as a pioneer for establishment of natural polysaccharide-based shear thinning hydrogels for possible application in various sectors.					
32439445	5	4	theme	remarkable	632:641	arg1	properties					654:663	remarkable mechanical properties	632:663	remarkable mechanical properties	632:663	Frequency sweeps indicated that remarkable mechanical properties were exhibited by the composite hydrogels.					
32439445	2	5	theme	chemical	285:292	arg1	toxicity					294:301	chemical toxicity	285:301	chemical toxicity	285:301	However, the application of such chemically modified hydrogels is limited due to their high gelation time and chemical toxicity.					
32439445	4	6	theme	rheological	488:498	arg1	analysis					500:507	Comprehensive rheological analysis	474:507	Comprehensive rheological analysis	474:507	Comprehensive rheological analysis was conducted to understand the shear thinning and self-healing behaviour of the hydrogel.					
32439445	5	7	theme	mechanical	643:652	arg1	properties					654:663	remarkable mechanical properties	632:663	remarkable mechanical properties	632:663	Frequency sweeps indicated that remarkable mechanical properties were exhibited by the composite hydrogels.					
32439445	6	8	theme	injectability	755:767	arg1	ease					747:750	an ease	744:750	an ease of injectability	744:767	Furthermore, the hydrogel exhibited an ease of injectability as demonstrated by strain sweep experiments.					
32439445	4	9	theme	Comprehensive	474:486	arg1	analysis					500:507	Comprehensive rheological analysis	474:507	Comprehensive rheological analysis	474:507	Comprehensive rheological analysis was conducted to understand the shear thinning and self-healing behaviour of the hydrogel.					
32439445	7	10	theme	thinning	914:921	arg1	hydrogels					923:931	natural polysaccharide-based shear thinning hydrogels	879:931	natural polysaccharide-based shear thinning hydrogels for possible application in various sectors	879:975	This study can, therefore, act as a pioneer for establishment of natural polysaccharide-based shear thinning hydrogels for possible application in various sectors.					
32439445	0	11	theme	novel	48:52	arg1	levan					69:73	a novel shear thinning levan	46:73	a novel shear thinning levan	46:73	Synthesis and rheological characterization of a novel shear thinning levan gellan hydrogel.					
32439445	2	12	theme	gelation	267:274	arg1	time					276:279	their high gelation time	256:279	their high gelation time	256:279	However, the application of such chemically modified hydrogels is limited due to their high gelation time and chemical toxicity.					
32439445	7	13	theme	natural	879:885	arg1	hydrogels					923:931	natural polysaccharide-based shear thinning hydrogels	879:931	natural polysaccharide-based shear thinning hydrogels for possible application in various sectors	879:975	This study can, therefore, act as a pioneer for establishment of natural polysaccharide-based shear thinning hydrogels for possible application in various sectors.					
32439445	3	14	theme	quick	379:383	arg1	setting					385:391	a novel quick setting	371:391	a novel quick setting	371:391	The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.					
32439445	3	14	theme	quick	379:383	arg1	hydrogel					404:411	non-toxic hydrogel	394:411	non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan	394:471	The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.					
32439445	5	15	theme	Frequency	600:608	arg1	sweeps					610:615	Frequency sweeps	600:615	Frequency sweeps	600:615	Frequency sweeps indicated that remarkable mechanical properties were exhibited by the composite hydrogels.					
32439445	7	16	theme	polysaccharide-based	887:906	arg1	hydrogels					923:931	natural polysaccharide-based shear thinning hydrogels	879:931	natural polysaccharide-based shear thinning hydrogels for possible application in various sectors	879:975	This study can, therefore, act as a pioneer for establishment of natural polysaccharide-based shear thinning hydrogels for possible application in various sectors.					
32439445	1	17	theme	Shear	92:96	arg1	hydrogels					107:115	Shear thinning hydrogels	92:115	Shear thinning hydrogels	92:115	Shear thinning hydrogels are majorly employed in biomedical and cosmetic industry.					
32439445	3	18	theme	biodegradable	425:437	arg1	polysaccharides					439:453	biodegradable polysaccharides	425:453	biodegradable polysaccharides	425:453	The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.					
32439445	3	18	theme	biodegradable	425:437	arg1	gellan					466:471	gellan	466:471	gellan	466:471	The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.					
32439445	3	18	theme	biodegradable	425:437	arg1	levan					456:460	levan	456:460	levan	456:460	The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.					
32439445	1	19	theme	thinning	98:105	arg1	hydrogels					107:115	Shear thinning hydrogels	92:115	Shear thinning hydrogels	92:115	Shear thinning hydrogels are majorly employed in biomedical and cosmetic industry.					
32439445	3	20	theme	novel	373:377	arg1	setting					385:391	a novel quick setting	371:391	a novel quick setting	371:391	The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.					
32439445	3	20	theme	novel	373:377	arg1	hydrogel					404:411	non-toxic hydrogel	394:411	non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan	394:471	The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.					
32439445	4	21	theme	hydrogel	590:597	arg1	thinning					547:554	shear thinning	541:554	shear thinning	541:554	Comprehensive rheological analysis was conducted to understand the shear thinning and self-healing behaviour of the hydrogel.					
32439445	4	21	theme	hydrogel	590:597	arg1	behaviour					573:581	self-healing behaviour	560:581	self-healing behaviour	560:581	Comprehensive rheological analysis was conducted to understand the shear thinning and self-healing behaviour of the hydrogel.					
32439445	7	22	theme	shear	908:912	arg1	hydrogels					923:931	natural polysaccharide-based shear thinning hydrogels	879:931	natural polysaccharide-based shear thinning hydrogels for possible application in various sectors	879:975	This study can, therefore, act as a pioneer for establishment of natural polysaccharide-based shear thinning hydrogels for possible application in various sectors.					
32439445	0	23	theme	shear	54:58	arg1	levan					69:73	a novel shear thinning levan	46:73	a novel shear thinning levan	46:73	Synthesis and rheological characterization of a novel shear thinning levan gellan hydrogel.					
32439445	4	24	theme	shear	541:545	arg1	thinning					547:554	shear thinning	541:554	shear thinning	541:554	Comprehensive rheological analysis was conducted to understand the shear thinning and self-healing behaviour of the hydrogel.					
32439445	2	25	theme	hydrogels	228:236	arg1	application					188:198	the application	184:198	the application of such chemically modified hydrogels	184:236	However, the application of such chemically modified hydrogels is limited due to their high gelation time and chemical toxicity.					
32439445	3	26	theme	non-toxic	394:402	arg1	setting					385:391	a novel quick setting	371:391	a novel quick setting	371:391	The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.					
32439445	3	26	theme	non-toxic	394:402	arg1	hydrogel					404:411	non-toxic hydrogel	394:411	non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan	394:471	The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.					
32439445	4	27	theme	self-healing	560:571	arg1	behaviour					573:581	self-healing behaviour	560:581	self-healing behaviour	560:581	Comprehensive rheological analysis was conducted to understand the shear thinning and self-healing behaviour of the hydrogel.					
32439445	3	28	theme	present	308:314	arg1	study					316:320	The present study	304:320	The present study	304:320	The present study attempts to address these issues by synthesizing a novel quick setting, non-toxic hydrogel composed of biodegradable polysaccharides, levan and gellan.					
32439445	2	29	theme	modified	219:226	arg1	hydrogels					228:236	such chemically modified hydrogels	203:236	such chemically modified hydrogels	203:236	However, the application of such chemically modified hydrogels is limited due to their high gelation time and chemical toxicity.					
32439445	2	30	theme	such	203:206	arg1	hydrogels					228:236	such chemically modified hydrogels	203:236	such chemically modified hydrogels	203:236	However, the application of such chemically modified hydrogels is limited due to their high gelation time and chemical toxicity.					
32439445	2	31	theme	high	262:265	arg1	time					276:279	their high gelation time	256:279	their high gelation time	256:279	However, the application of such chemically modified hydrogels is limited due to their high gelation time and chemical toxicity.					
32439445	6	32	theme	sweep	795:799	arg1	experiments					801:811	strain sweep experiments	788:811	strain sweep experiments	788:811	Furthermore, the hydrogel exhibited an ease of injectability as demonstrated by strain sweep experiments.					
32439445	7	33	from	application	946:956	arg1	sectors					969:975	various sectors	961:975	various sectors	961:975	This study can, therefore, act as a pioneer for establishment of natural polysaccharide-based shear thinning hydrogels for possible application in various sectors.					
32439445	6	34	theme	strain	788:793	arg1	experiments					801:811	strain sweep experiments	788:811	strain sweep experiments	788:811	Furthermore, the hydrogel exhibited an ease of injectability as demonstrated by strain sweep experiments.					
32439445	0	35	theme	levan	69:73	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and rheological characterization of a novel shear thinning levan gellan hydrogel.					
32439445	0	35	theme	levan	69:73	arg1	characterization					26:41	rheological characterization	14:41	rheological characterization	14:41	Synthesis and rheological characterization of a novel shear thinning levan gellan hydrogel.					
32439445	1	36	theme	biomedical	141:150	arg1	industry					165:172	biomedical and cosmetic industry	141:172	biomedical and cosmetic industry	141:172	Shear thinning hydrogels are majorly employed in biomedical and cosmetic industry.					
32439445	7	37	theme	various	961:967	arg1	sectors					969:975	various sectors	961:975	various sectors	961:975	This study can, therefore, act as a pioneer for establishment of natural polysaccharide-based shear thinning hydrogels for possible application in various sectors.					
32439445	0	38	theme	thinning	60:67	arg1	levan					69:73	a novel shear thinning levan	46:73	a novel shear thinning levan	46:73	Synthesis and rheological characterization of a novel shear thinning levan gellan hydrogel.					
32439445	0	39	theme	rheological	14:24	arg1	characterization					26:41	rheological characterization	14:41	rheological characterization	14:41	Synthesis and rheological characterization of a novel shear thinning levan gellan hydrogel.					
32439445	7	40	theme	possible	937:944	arg1	application					946:956	possible application	937:956	possible application in various sectors	937:975	This study can, therefore, act as a pioneer for establishment of natural polysaccharide-based shear thinning hydrogels for possible application in various sectors.					
34158109	9	0	link	RG-II-derived	1585:1597	arg1	oligosaccharides					1599:1614	RG-II-derived oligosaccharides	1585:1614	RG-II-derived oligosaccharides	1585:1614	We used electrospray-MS techniques to identify non-glycosyl modifications including methyl-ethers, methyl-esters, and acetyl-esters on RG-II-derived oligosaccharides.					
34158109	7	1	theme	-based	1105:1110	arg1	techniques					1112:1121	mass spectrometry (MS)-based techniques	1083:1121	mass spectrometry (MS)-based techniques	1083:1121	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	3	2	theme	recent	416:421	arg1	years					423:427	recent years	416:427	recent years	416:427	In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation.					
34158109	7	3	theme	spectrometry	1088:1099	arg1	techniques					1112:1121	mass spectrometry (MS)-based techniques	1083:1121	mass spectrometry (MS)-based techniques	1083:1121	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	10	4	theme	RG-II	1770:1774	arg1	studies					1789:1795	the RG-II dimerization studies	1766:1795	the RG-II dimerization studies performed using size-exclusion chromatography	1766:1841	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	1	5	theme	polysaccharides	167:181	arg1	BACKGROUND					124:133	BACKGROUND	124:133	BACKGROUND In plants	124:143	BACKGROUND In plants, a large diversity of polysaccharides comprise the cell wall.					
34158109	1	5	theme	polysaccharides	167:181	arg1	diversity					154:162	a large diversity	146:162	a large diversity of polysaccharides	146:181	BACKGROUND In plants, a large diversity of polysaccharides comprise the cell wall.					
34158109	12	6	theme	necessary	2021:2029	arg1	steps					2015:2019	the steps	2011:2019	the steps necessary to isolate and characterize polysaccharides using chromatographic, MS, and NMR techniques	2011:2119	In this work, we used RG-II as a model to demonstrate the steps necessary to isolate and characterize polysaccharides using chromatographic, MS, and NMR techniques.					
34158109	9	7	from	modifications	1510:1522	arg1	oligosaccharides					1599:1614	RG-II-derived oligosaccharides	1585:1614	RG-II-derived oligosaccharides	1585:1614	We used electrospray-MS techniques to identify non-glycosyl modifications including methyl-ethers, methyl-esters, and acetyl-esters on RG-II-derived oligosaccharides.					
34158109	5	8	theme	principles	715:724	arg1	workflow					703:710	a workflow	701:710	a workflow of principles relevant for the characterization of polysaccharide primary structure	701:794	Herein, we present a workflow of principles relevant for the characterization of polysaccharide primary structure using nature's most complex polysaccharide, rhamnogalacturonan-II (RG-II), as a model.					
34158109	6	9	theme	duckweed	939:946	arg1	walls					953:957	duckweed cell walls	939:957	duckweed cell walls	939:957	RESULTS We outline how to isolate RG-II from celery and duckweed cell walls and from red wine using chemical or enzymatic treatments coupled with size-exclusion chromatography.					
34158109	10	10	theme	resonance	1671:1679	arg1	1H-NMR					1695:1700	1H-NMR	1695:1700	1H-NMR	1695:1700	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	10	10	theme	resonance	1671:1679	arg1	spectroscopy					1681:1692	proton nuclear magnetic resonance spectroscopy	1647:1692	proton nuclear magnetic resonance spectroscopy (1H-NMR)	1647:1701	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	10	11	theme	nuclear	1654:1660	arg1	resonance					1671:1679	proton nuclear magnetic resonance	1647:1679	proton nuclear magnetic resonance spectroscopy (1H-NMR)	1647:1701	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	11	12	theme	structures	1896:1905	arg1	complexity					1860:1869	The complexity	1856:1869	The complexity of pectic polysaccharide structures	1856:1905	CONCLUSIONS The complexity of pectic polysaccharide structures has hampered efforts aimed at their valorization.					
34158109	13	13	theme	saccharide	2185:2194	arg1	structures					2196:2205	other saccharide structures	2179:2205	other saccharide structures	2179:2205	The principles can be applied to the characterization of other saccharide structures and will help inform researchers on how saccharide structure relates to functional properties in the future.					
34158109	7	14	theme	glycosyl	1140:1147	arg1	residue					1149:1155	the glycosyl residue and linkage compositions	1136:1180	residue	1149:1155	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	7	14	theme	glycosyl	1140:1147	arg1	RG-II					1196:1200	the intact RG-II	1185:1200	the intact RG-II	1185:1200	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	11	15	theme	pectic	1874:1879	arg1	structures					1896:1905	pectic polysaccharide structures	1874:1905	pectic polysaccharide structures	1874:1905	CONCLUSIONS The complexity of pectic polysaccharide structures has hampered efforts aimed at their valorization.					
34158109	2	16	theme	major	212:216	arg1	type					218:221	Each major type	207:221	Each major type	207:221	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	2	17	theme	polysaccharide	242:255	arg1	type					218:221	Each major type	207:221	Each major type	207:221	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	6	18	theme	enzymatic	995:1003	arg1	treatments					1005:1014	chemical or enzymatic treatments	983:1014	chemical or enzymatic treatments coupled with size-exclusion chromatography	983:1057	RESULTS We outline how to isolate RG-II from celery and duckweed cell walls and from red wine using chemical or enzymatic treatments coupled with size-exclusion chromatography.					
34158109	12	19	theme	NMR	2106:2108	arg1	techniques					2110:2119	chromatographic, MS, and NMR techniques	2081:2119	techniques	2110:2119	In this work, we used RG-II as a model to demonstrate the steps necessary to isolate and characterize polysaccharides using chromatographic, MS, and NMR techniques.					
34158109	0	20	theme	cell	70:73	arg1	walls					75:79	plant cell walls	64:79	plant cell walls	64:79	Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II.					
34158109	11	21	dep	CONCLUSIONS	1844:1854	arg1	hampered					1911:1918	hampered	1911:1918	has hampered efforts aimed at their valorization	1907:1954	CONCLUSIONS The complexity of pectic polysaccharide structures has hampered efforts aimed at their valorization.					
34158109	2	22	theme	cell	232:235	arg1	polysaccharide					242:255	plant cell wall polysaccharide	226:255	plant cell wall polysaccharide	226:255	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	6	23	theme	chemical	983:990	arg1	treatments					1005:1014	chemical or enzymatic treatments	983:1014	chemical or enzymatic treatments coupled with size-exclusion chromatography	983:1057	RESULTS We outline how to isolate RG-II from celery and duckweed cell walls and from red wine using chemical or enzymatic treatments coupled with size-exclusion chromatography.					
34158109	5	24	theme	polysaccharide	763:776	arg1	structure					786:794	polysaccharide primary structure	763:794	polysaccharide primary structure	763:794	Herein, we present a workflow of principles relevant for the characterization of polysaccharide primary structure using nature's most complex polysaccharide, rhamnogalacturonan-II (RG-II), as a model.					
34158109	8	25	theme	Wolffiella	1342:1351	arg1	repanda					1353:1359	the duckweed Wolffiella repanda	1329:1359	the duckweed Wolffiella repanda	1329:1359	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	0	26	theme	case	84:87	arg1	study					89:93	a case study	82:93	Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II.	0:122	Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II.					
34158109	11	27	dep	efforts	1920:1926	arg1	aimed					1928:1932	aimed	1928:1932	efforts aimed at their valorization	1920:1954	CONCLUSIONS The complexity of pectic polysaccharide structures has hampered efforts aimed at their valorization.					
34158109	9	28	used	used	1453:1456	arg2	We					1450:1451	We	1450:1451	We	1450:1451	We used electrospray-MS techniques to identify non-glycosyl modifications including methyl-ethers, methyl-esters, and acetyl-esters on RG-II-derived oligosaccharides.					
34158109	13	29	from	properties	2290:2299	arg1	future					2308:2313	future	2308:2313	future	2308:2313	The principles can be applied to the characterization of other saccharide structures and will help inform researchers on how saccharide structure relates to functional properties in the future.					
34158109	10	30	theme	spectroscopy	1681:1692	arg1	utility					1636:1642	the utility	1632:1642	the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography	1632:1841	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	7	31	theme	linkage	1161:1167	arg1	compositions					1169:1180	the glycosyl residue and linkage compositions	1136:1180	compositions	1169:1180	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	6	32	theme	size-exclusion	1029:1042	arg1	chromatography					1044:1057	size-exclusion chromatography	1029:1057	size-exclusion chromatography	1029:1057	RESULTS We outline how to isolate RG-II from celery and duckweed cell walls and from red wine using chemical or enzymatic treatments coupled with size-exclusion chromatography.					
34158109	5	33	theme	structure	786:794	arg1	characterization					743:758	the characterization	739:758	the characterization of polysaccharide primary structure	739:794	Herein, we present a workflow of principles relevant for the characterization of polysaccharide primary structure using nature's most complex polysaccharide, rhamnogalacturonan-II (RG-II), as a model.					
34158109	8	34	theme	chain	1405:1409	arg1	B					1411:1411	side chain B	1400:1411	side chain B	1400:1411	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	10	35	theme	RG-II	1742:1746	arg1	structure					1722:1730	the structure	1718:1730	the structure of intact RG-II	1718:1746	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	2	36	contain	has	306:308	arg2	functions					334:342	functions	334:342	functions	334:342	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	2	36	contain	has	306:308	arg1	type					218:221	Each major type	207:221	Each major type	207:221	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	2	36	contain	has	306:308	arg2	structures					319:328	distinct structures	310:328	distinct structures	310:328	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	2	37	theme	distinct	310:317	arg1	structures					319:328	distinct structures	310:328	distinct structures	310:328	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	12	38	theme	chromatographic	2081:2095	arg1	MS					2098:2099	chromatographic, MS, and NMR techniques	2081:2119	MS	2098:2099	In this work, we used RG-II as a model to demonstrate the steps necessary to isolate and characterize polysaccharides using chromatographic, MS, and NMR techniques.					
34158109	0	39	dep	Protocols	0:8	arg1	study					89:93	a case study	82:93	Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II.	0:122	Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II.					
34158109	6	40	dep	RESULTS	883:889	arg1	outline					894:900	outline	894:900	outline how to isolate RG-II from celery and duckweed cell walls and from red wine using chemical or enzymatic treatments coupled with size-exclusion chromatography	894:1057	RESULTS We outline how to isolate RG-II from celery and duckweed cell walls and from red wine using chemical or enzymatic treatments coupled with size-exclusion chromatography.					
34158109	12	41	used	used	1974:1977	arg2	we					1971:1972	we	1971:1972	we	1971:1972	In this work, we used RG-II as a model to demonstrate the steps necessary to isolate and characterize polysaccharides using chromatographic, MS, and NMR techniques.					
34158109	3	42	theme	expanding	490:498	arg1	roles					500:504	its expanding roles	486:504	its expanding roles in biomass deconstruction, food and material science, and environmental remediation	486:588	In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation.					
34158109	7	43	theme	special	1241:1247	arg1	considerations					1249:1262	special considerations	1241:1262	special considerations for labile monosaccharides	1241:1289	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	3	44	from	roles	500:504	arg1	food					533:536	food	533:536	food	533:536	In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation.					
34158109	3	44	from	roles	500:504	arg1	deconstruction					517:530	biomass deconstruction	509:530	biomass deconstruction	509:530	In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation.					
34158109	3	44	from	roles	500:504	arg1	remediation					578:588	environmental remediation	564:588	environmental remediation	564:588	In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation.					
34158109	7	45	theme	mass	1083:1086	arg1	MS					1102:1103	MS	1102:1103	MS	1102:1103	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	7	45	theme	mass	1083:1086	arg1	spectrometry					1088:1099	mass spectrometry	1083:1099	mass spectrometry (MS)-based techniques	1083:1121	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	10	46	theme	dimerization	1776:1787	arg1	studies					1789:1795	the RG-II dimerization studies	1766:1795	the RG-II dimerization studies performed using size-exclusion chromatography	1766:1841	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	4	47	theme	incomplete	643:652	arg1	knowledge					654:662	our incomplete knowledge	639:662	our incomplete knowledge of its structure	639:679	However, pectin utilization has been limited by our incomplete knowledge of its structure.					
34158109	10	48	dep	utility	1636:1642	arg1	complement					1755:1764	complement	1755:1764	to complement the RG-II dimerization studies performed using size-exclusion chromatography	1752:1841	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	10	48	dep	utility	1636:1642	arg1	investigate					1706:1716	investigate	1706:1716	to investigate the structure of intact RG-II	1703:1746	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	10	49	theme	size-exclusion	1813:1826	arg1	chromatography					1828:1841	size-exclusion chromatography	1813:1841	size-exclusion chromatography	1813:1841	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	1	50	theme	cell	196:199	arg1	wall					201:204	the cell wall	192:204	the cell wall	192:204	BACKGROUND In plants, a large diversity of polysaccharides comprise the cell wall.					
34158109	6	51	theme	cell	948:951	arg1	walls					953:957	duckweed cell walls	939:957	duckweed cell walls	939:957	RESULTS We outline how to isolate RG-II from celery and duckweed cell walls and from red wine using chemical or enzymatic treatments coupled with size-exclusion chromatography.					
34158109	10	52	theme	magnetic	1662:1669	arg1	resonance					1671:1679	proton nuclear magnetic resonance	1647:1679	proton nuclear magnetic resonance spectroscopy (1H-NMR)	1647:1701	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	13	53	theme	other	2179:2183	arg1	structures					2196:2205	other saccharide structures	2179:2205	other saccharide structures	2179:2205	The principles can be applied to the characterization of other saccharide structures and will help inform researchers on how saccharide structure relates to functional properties in the future.					
34158109	2	54	theme	plant	226:230	arg1	polysaccharide					242:255	plant cell wall polysaccharide	226:255	plant cell wall polysaccharide	226:255	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	9	55	theme	electrospray-MS	1458:1472	arg1	techniques					1474:1483	electrospray-MS techniques	1458:1483	electrospray-MS techniques	1458:1483	We used electrospray-MS techniques to identify non-glycosyl modifications including methyl-ethers, methyl-esters, and acetyl-esters on RG-II-derived oligosaccharides.					
34158109	0	56	from	walls	75:79	arg1	polysaccharides					43:57	polysaccharides	43:57	polysaccharides from plant cell walls	43:79	Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II.					
34158109	10	57	theme	proton	1647:1652	arg1	resonance					1671:1679	proton nuclear magnetic resonance	1647:1679	proton nuclear magnetic resonance spectroscopy (1H-NMR)	1647:1701	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	13	58	theme	structures	2196:2205	arg1	characterization					2159:2174	the characterization	2155:2174	the characterization of other saccharide structures	2155:2205	The principles can be applied to the characterization of other saccharide structures and will help inform researchers on how saccharide structure relates to functional properties in the future.					
34158109	3	59	theme	environmental	564:576	arg1	remediation					578:588	environmental remediation	564:588	environmental remediation	564:588	In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation.					
34158109	11	60	theme	polysaccharide	1881:1894	arg1	structures					1896:1905	pectic polysaccharide structures	1874:1905	pectic polysaccharide structures	1874:1905	CONCLUSIONS The complexity of pectic polysaccharide structures has hampered efforts aimed at their valorization.					
34158109	9	61	theme	non-glycosyl	1497:1508	arg1	acetyl-esters					1568:1580	acetyl-esters	1568:1580	acetyl-esters	1568:1580	We used electrospray-MS techniques to identify non-glycosyl modifications including methyl-ethers, methyl-esters, and acetyl-esters on RG-II-derived oligosaccharides.					
34158109	9	61	theme	non-glycosyl	1497:1508	arg1	methyl-ethers					1534:1546	methyl-ethers	1534:1546	methyl-ethers	1534:1546	We used electrospray-MS techniques to identify non-glycosyl modifications including methyl-ethers, methyl-esters, and acetyl-esters on RG-II-derived oligosaccharides.					
34158109	9	61	theme	non-glycosyl	1497:1508	arg1	modifications					1510:1522	non-glycosyl modifications	1497:1522	non-glycosyl modifications including methyl-ethers, methyl-esters, and acetyl-esters on RG-II-derived oligosaccharides	1497:1614	We used electrospray-MS techniques to identify non-glycosyl modifications including methyl-ethers, methyl-esters, and acetyl-esters on RG-II-derived oligosaccharides.					
34158109	9	61	theme	non-glycosyl	1497:1508	arg1	methyl-esters					1549:1561	methyl-esters	1549:1561	methyl-esters	1549:1561	We used electrospray-MS techniques to identify non-glycosyl modifications including methyl-ethers, methyl-esters, and acetyl-esters on RG-II-derived oligosaccharides.					
34158109	2	62	theme	wall	237:240	arg1	polysaccharide					242:255	plant cell wall polysaccharide	226:255	plant cell wall polysaccharide	226:255	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	4	63	theme	structure	671:679	arg1	knowledge					654:662	our incomplete knowledge	639:662	our incomplete knowledge of its structure	639:679	However, pectin utilization has been limited by our incomplete knowledge of its structure.					
34158109	7	64	theme	intact	1189:1194	arg1	RG-II					1196:1200	the intact RG-II	1185:1200	the intact RG-II	1185:1200	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	7	65	theme	RG-II	1196:1200	arg1	residue					1149:1155	the glycosyl residue and linkage compositions	1136:1180	residue	1149:1155	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	7	65	theme	RG-II	1196:1200	arg1	compositions					1169:1180	the glycosyl residue and linkage compositions	1136:1180	compositions	1169:1180	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	7	65	theme	RG-II	1196:1200	arg1	RG-II					1196:1200	the intact RG-II	1185:1200	the intact RG-II	1185:1200	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	1	66	theme	large	148:152	arg1	BACKGROUND					124:133	BACKGROUND	124:133	BACKGROUND In plants	124:143	BACKGROUND In plants, a large diversity of polysaccharides comprise the cell wall.					
34158109	1	66	theme	large	148:152	arg1	diversity					154:162	a large diversity	146:162	a large diversity of polysaccharides	146:181	BACKGROUND In plants, a large diversity of polysaccharides comprise the cell wall.					
34158109	6	67	theme	red	968:970	arg1	wine					972:975	red wine	968:975	red wine using chemical or enzymatic treatments coupled with size-exclusion chromatography	968:1057	RESULTS We outline how to isolate RG-II from celery and duckweed cell walls and from red wine using chemical or enzymatic treatments coupled with size-exclusion chromatography.					
34158109	8	68	theme	duckweed	1333:1340	arg1	repanda					1353:1359	the duckweed Wolffiella repanda	1329:1359	the duckweed Wolffiella repanda	1329:1359	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	5	69	theme	relevant	726:733	arg1	principles					715:724	principles	715:724	principles relevant for the characterization of polysaccharide primary structure	715:794	Herein, we present a workflow of principles relevant for the characterization of polysaccharide primary structure using nature's most complex polysaccharide, rhamnogalacturonan-II (RG-II), as a model.					
34158109	3	70	theme	pectin	430:435	arg1	valorization					437:448	pectin valorization	430:448	pectin valorization	430:448	In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation.					
34158109	9	71	theme	RG-II-derived	1585:1597	arg1	oligosaccharides					1599:1614	RG-II-derived oligosaccharides	1585:1614	RG-II-derived oligosaccharides	1585:1614	We used electrospray-MS techniques to identify non-glycosyl modifications including methyl-ethers, methyl-esters, and acetyl-esters on RG-II-derived oligosaccharides.					
34158109	8	72	theme	arabinopyranosyl	1365:1380	arg1	residue					1389:1395	the arabinopyranosyl (Arap) residue	1361:1395	the arabinopyranosyl (Arap) residue of side chain B	1361:1411	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	8	72	theme	arabinopyranosyl	1365:1380	arg1	B					1411:1411	side chain B	1400:1411	side chain B	1400:1411	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	2	73	theme	wall	363:366	arg1	mechanics					368:376	wall mechanics	363:376	wall mechanics	363:376	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	5	74	theme	complex	816:822	arg1	model					876:880	a model	874:880	a model	874:880	Herein, we present a workflow of principles relevant for the characterization of polysaccharide primary structure using nature's most complex polysaccharide, rhamnogalacturonan-II (RG-II), as a model.					
34158109	5	74	theme	complex	816:822	arg1	rhamnogalacturonan-II					840:860	rhamnogalacturonan-II	840:860	rhamnogalacturonan-II (RG-II)	840:868	Herein, we present a workflow of principles relevant for the characterization of polysaccharide primary structure using nature's most complex polysaccharide, rhamnogalacturonan-II (RG-II), as a model.					
34158109	5	74	theme	complex	816:822	arg1	polysaccharide					824:837	nature's most complex polysaccharide	802:837	nature's most complex polysaccharide	802:837	Herein, we present a workflow of principles relevant for the characterization of polysaccharide primary structure using nature's most complex polysaccharide, rhamnogalacturonan-II (RG-II), as a model.					
34158109	1	75	from	BACKGROUND	124:133	arg1	plants					138:143	plants	138:143	plants	138:143	BACKGROUND In plants, a large diversity of polysaccharides comprise the cell wall.					
34158109	3	76	dep	food	533:536	arg1	science					551:557	science	551:557	science	551:557	In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation.					
34158109	8	77	theme	Arap	1383:1386	arg1	residue					1389:1395	the arabinopyranosyl (Arap) residue	1361:1395	the arabinopyranosyl (Arap) residue of side chain B	1361:1411	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	8	77	theme	Arap	1383:1386	arg1	B					1411:1411	side chain B	1400:1411	side chain B	1400:1411	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	3	78	theme	much	464:467	arg1	attention					469:477	much attention	464:477	much attention	464:477	In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation.					
34158109	5	79	theme	primary	778:784	arg1	structure					786:794	polysaccharide primary structure	763:794	polysaccharide primary structure	763:794	Herein, we present a workflow of principles relevant for the characterization of polysaccharide primary structure using nature's most complex polysaccharide, rhamnogalacturonan-II (RG-II), as a model.					
34158109	2	80	theme	plant	392:396	arg1	morphogenesis					398:410	plant morphogenesis	392:410	plant morphogenesis	392:410	Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis.					
34158109	0	81	theme	plant	64:68	arg1	walls					75:79	plant cell walls	64:79	plant cell walls	64:79	Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II.					
34158109	8	82	theme	side	1400:1403	arg1	B					1411:1411	side chain B	1400:1411	side chain B	1400:1411	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	10	83	theme	intact	1735:1740	arg1	RG-II					1742:1746	intact RG-II	1735:1746	intact RG-II	1735:1746	We then showed the utility of proton nuclear magnetic resonance spectroscopy (1H-NMR) to investigate the structure of intact RG-II and to complement the RG-II dimerization studies performed using size-exclusion chromatography.					
34158109	13	84	theme	functional	2279:2288	arg1	properties					2290:2299	functional properties	2279:2299	functional properties in the future	2279:2313	The principles can be applied to the characterization of other saccharide structures and will help inform researchers on how saccharide structure relates to functional properties in the future.					
34158109	7	85	theme	labile	1268:1273	arg1	monosaccharides					1275:1289	labile monosaccharides	1268:1289	labile monosaccharides	1268:1289	From there, we applied mass spectrometry (MS)-based techniques to determine the glycosyl residue and linkage compositions of the intact RG-II and derived oligosaccharides including special considerations for labile monosaccharides.					
34158109	4	86	theme	pectin	600:605	arg1	utilization					607:617	pectin utilization	600:617	pectin utilization	600:617	However, pectin utilization has been limited by our incomplete knowledge of its structure.					
34158109	8	87	theme	B	1411:1411	arg1	residue					1389:1395	the arabinopyranosyl (Arap) residue	1361:1395	the arabinopyranosyl (Arap) residue of side chain B	1361:1411	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	8	87	theme	B	1411:1411	arg1	B					1411:1411	side chain B	1400:1411	side chain B	1400:1411	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	8	88	with	O-2	1431:1433	arg1	rhamnose					1440:1447	rhamnose	1440:1447	rhamnose	1440:1447	In doing so, we demonstrated that in the duckweed Wolffiella repanda the arabinopyranosyl (Arap) residue of side chain B is substituted at O-2 with rhamnose.					
34158109	3	89	theme	biomass	509:515	arg1	deconstruction					517:530	biomass deconstruction	509:530	biomass deconstruction	509:530	In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation.					
34158109	13	90	theme	saccharide	2247:2256	arg1	structure					2258:2266	saccharide structure	2247:2266	saccharide structure	2247:2266	The principles can be applied to the characterization of other saccharide structures and will help inform researchers on how saccharide structure relates to functional properties in the future.					
32321762	4	0	theme	human	765:769	arg1	brains					771:776	human brains	765:776	human brains	765:776	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	3	1	theme	sialic	599:604	arg1	type					619:622	the sialic acid linkage type	595:622	the sialic acid linkage type in the GPI side chain	595:644	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	6	2	theme	reported	1123:1130	arg1	Ser231					1100:1105	Ser231	1100:1105	Ser231	1100:1105	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	2	theme	reported	1123:1130	arg1	site					1134:1137	the previously reported ω site	1108:1137	the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain	1108:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	2	theme	reported	1123:1130	arg1	PrPC					1157:1160	Syrian hamster PrPC	1142:1160	Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain	1142:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	3	3	theme	GPI	631:633	arg1	chain					640:644	the GPI side chain	627:644	the GPI side chain	627:644	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	5	4	from	site	1017:1020	arg1	PrPC					1025:1028	PrPC	1025:1028	PrPC	1025:1028	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	1	5	theme	protein	243:249	arg1	form					225:228	the aggregated scrapie form	202:228	the aggregated scrapie form of the prion protein (PrPSc)	202:257	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	4	6	theme	human	740:744	arg1	PrPC					746:749	human PrPC	740:749	human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene	740:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	8	7	theme	sialic	1658:1663	arg1	acid					1665:1668	the sialic acid	1654:1668	the sialic acid linkage type	1654:1681	In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.					
32321762	3	8	dep	location	530:537	arg1	the					526:528	the	526:528	the	526:528	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	4	9	theme	mouse	808:812	arg1	model					814:818	a knock-in mouse model	797:818	a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene	797:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	0	10	theme	human	86:90	arg1	protein					98:104	human prion protein	86:104	human prion protein	86:104	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	6	11	theme	side	1307:1310	arg1	chain					1312:1316	the side chain	1303:1316	the side chain	1303:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	2	12	from	acid	433:436	arg1	chain					452:456	a GPI side chain	441:456	a GPI side chain	441:456	The glycosylphosphatidylinositol (GPI) anchor of PrPC is involved in prion disease pathogenesis, and especially sialic acid in a GPI side chain reportedly affects PrPC conversion.					
32321762	6	13	theme	PrPC	1157:1160	arg1	Ser231					1100:1105	Ser231	1100:1105	Ser231	1100:1105	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	13	theme	PrPC	1157:1160	arg1	site					1134:1137	the previously reported ω site	1108:1137	the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain	1108:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	13	theme	PrPC	1157:1160	arg1	PrPC					1157:1160	Syrian hamster PrPC	1142:1160	Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain	1142:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	5	14	theme	PrPC	952:955	arg1	analysis					934:941	LC-electrospray ionization-MS analysis	904:941	LC-electrospray ionization-MS analysis of human PrPC from both biological sources	904:984	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	3	15	from	type	619:622	arg1	chain					640:644	the GPI side chain	627:644	the GPI side chain	627:644	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	6	16	theme	Syrian	1142:1147	arg1	PrPC					1157:1160	Syrian hamster PrPC	1142:1160	Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain	1142:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	0	17	from	site	78:81	arg1	protein					98:104	human prion protein	86:104	human prion protein	86:104	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	8	18	theme	linkage	1670:1676	arg1	type					1678:1681	linkage type	1670:1681	the sialic acid linkage type	1654:1681	In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.					
32321762	5	19	theme	ionization-MS	920:932	arg1	analysis					934:941	LC-electrospray ionization-MS analysis	904:941	LC-electrospray ionization-MS analysis of human PrPC from both biological sources	904:984	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	8	20	theme	GPI	1563:1565	arg1	structure					1574:1582	the GPI glycan structure	1559:1582	the GPI glycan structure	1559:1582	In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.					
32321762	7	21	theme	α2,3	1523:1526	arg1	linkage					1528:1534	an α2,3 linkage	1520:1534	an α2,3 linkage	1520:1534	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	2	22	theme	GPI	443:445	arg1	chain					452:456	a GPI side chain	441:456	a GPI side chain	441:456	The glycosylphosphatidylinositol (GPI) anchor of PrPC is involved in prion disease pathogenesis, and especially sialic acid in a GPI side chain reportedly affects PrPC conversion.					
32321762	5	23	from	analysis	934:941	arg1	sources					978:984	both biological sources	962:984	both biological sources	962:984	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	1	24	theme	prion	292:296	arg1	PrPC					307:310	PrPC	307:310	PrPC	307:310	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	1	24	theme	prion	292:296	arg1	protein					298:304	cellular prion protein	283:304	the cellular prion protein (PrPC)	279:311	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	7	25	theme	α2,3	1394:1397	arg1	linkage					1399:1405	α2,3 linkage	1394:1405	α2,3 linkage from α2,6 linkage	1394:1423	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	6	26	from	anchors	1196:1202	arg1	PrPCs					1213:1217	human PrPCs	1207:1217	human PrPCs	1207:1217	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	26	from	anchors	1196:1202	arg1	brains					1249:1254	human and knock-in mouse brains	1224:1254	human and knock-in mouse brains	1224:1254	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	7	27	from	linkage	1417:1423	arg1	linkage					1399:1405	α2,3 linkage	1394:1405	α2,3 linkage from α2,6 linkage	1394:1423	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	8	28	theme	human	1587:1591	arg1	PrPC					1593:1596	human PrPC	1587:1596	human PrPC	1587:1596	In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.					
32321762	1	29	theme	transmissible	126:138	arg1	diseases					113:120	Prion diseases	107:120	Prion diseases	107:120	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	1	29	theme	transmissible	126:138	arg1	disorders					166:174	transmissible, lethal neurodegenerative disorders	126:174	transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC)	126:311	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	6	30	from	Gly229	1056:1061	arg1	PrPC					1072:1075	human PrPC	1066:1075	human PrPC	1066:1075	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	0	31	theme	GPI	63:65	arg1	site					78:81	an unpredicted GPI attachment site	48:81	an unpredicted GPI attachment site in human prion protein	48:104	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	0	31	theme	GPI	63:65	arg1	acid					23:26	sialic acid	16:26	sialic acid	16:26	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	6	32	from	%	1179:1179	arg1	PrPCs					1213:1217	human PrPCs	1207:1217	human PrPCs	1207:1217	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	7	33	theme	alkylamidation	1356:1369	arg1	method					1371:1376	a sialic acid linkage-specific alkylamidation method	1325:1376	a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage	1325:1423	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	4	34	theme	prion	839:843	arg1	gene					860:863	the mouse prion protein (Prnp) gene	829:863	the mouse prion protein (Prnp) gene	829:863	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	3	35	from	structure	543:551	arg1	PrPC					580:583	human PrPC	574:583	human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein	574:697	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	7	36	theme	side	1478:1481	arg1	chain					1483:1487	PrPC's GPI side chain	1467:1487	PrPC's GPI side chain	1467:1487	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	4	37	theme	human	887:891	arg1	gene					898:901	the human PRNP gene	883:901	the human PRNP gene	883:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	8	38	dep	acid	1665:1668	arg1	type					1678:1681	linkage type	1670:1681	the sialic acid linkage type	1654:1681	In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.					
32321762	2	39	theme	GPI	348:350	arg1	anchor					353:358	The glycosylphosphatidylinositol (GPI) anchor	314:358	The glycosylphosphatidylinositol (GPI) anchor of PrPC	314:366	The glycosylphosphatidylinositol (GPI) anchor of PrPC is involved in prion disease pathogenesis, and especially sialic acid in a GPI side chain reportedly affects PrPC conversion.					
32321762	6	40	theme	knock-in	1234:1241	arg1	mouse					1243:1247	knock-in mouse	1234:1247	knock-in mouse	1234:1247	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	7	41	from	acid	1459:1462	arg1	chain					1483:1487	PrPC's GPI side chain	1467:1487	PrPC's GPI side chain	1467:1487	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	8	42	theme	amino	1620:1624	arg1	acid					1626:1629	the ω-site amino acid	1609:1629	the ω-site amino acid for GPI attachment	1609:1648	In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.					
32321762	2	43	theme	prion	383:387	arg1	pathogenesis					397:408	prion disease pathogenesis	383:408	prion disease pathogenesis	383:408	The glycosylphosphatidylinositol (GPI) anchor of PrPC is involved in prion disease pathogenesis, and especially sialic acid in a GPI side chain reportedly affects PrPC conversion.					
32321762	6	44	theme	human	1224:1228	arg1	brains					1249:1254	human and knock-in mouse brains	1224:1254	human and knock-in mouse brains	1224:1254	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	7	45	theme	sialic	1327:1332	arg1	method					1371:1376	a sialic acid linkage-specific alkylamidation method	1325:1376	a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage	1325:1423	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	2	46	theme	sialic	426:431	arg1	acid					433:436	especially sialic acid	415:436	especially sialic acid in a GPI side chain	415:456	The glycosylphosphatidylinositol (GPI) anchor of PrPC is involved in prion disease pathogenesis, and especially sialic acid in a GPI side chain reportedly affects PrPC conversion.					
32321762	8	47	theme	GPI	1635:1637	arg1	attachment					1639:1648	GPI attachment	1635:1648	GPI attachment	1635:1648	In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.					
32321762	3	48	theme	human	574:578	arg1	PrPC					580:583	human PrPC	574:583	human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein	574:697	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	4	49	theme	GPI	715:717	arg1	structures					726:735	GPI glycan structures	715:735	GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene	715:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	3	50	from	location	530:537	arg1	PrPC					580:583	human PrPC	574:583	human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein	574:697	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	2	51	theme	PrPC	363:366	arg1	anchor					353:358	The glycosylphosphatidylinositol (GPI) anchor	314:358	The glycosylphosphatidylinositol (GPI) anchor of PrPC	314:366	The glycosylphosphatidylinositol (GPI) anchor of PrPC is involved in prion disease pathogenesis, and especially sialic acid in a GPI side chain reportedly affects PrPC conversion.					
32321762	0	52	theme	acid	23:26	arg1	site					78:81	an unpredicted GPI attachment site	48:81	an unpredicted GPI attachment site in human prion protein	48:104	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	0	52	theme	acid	23:26	arg1	linkage					5:11	linkage	5:11	linkage of sialic acid to a GPI anchor	5:42	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	0	52	theme	acid	23:26	arg1	acid					23:26	sialic acid	16:26	sialic acid	16:26	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	5	53	theme	ω	1015:1015	arg1	Gly229					1001:1006	Gly229	1001:1006	Gly229	1001:1006	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	5	53	theme	ω	1015:1015	arg1	site					1017:1020	the ω site	1011:1020	the ω site in PrPC to which GPI is attached	1011:1053	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	7	54	attach	linked	1492:1497	arg2	acid					1459:1462	N-acetylneuraminic acid	1440:1462	N-acetylneuraminic acid in PrPC's GPI side chain	1440:1487	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	7	54	attach	linked	1492:1497	arg1	galactose					1502:1510	galactose	1502:1510	galactose	1502:1510	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	3	55	theme	anchor	564:569	arg1	structure					543:551	structure	543:551	structure	543:551	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	3	55	theme	anchor	564:569	arg1	location					530:537	location	530:537	location	530:537	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	6	56	from	acid	1295:1298	arg1	chain					1312:1316	the side chain	1303:1316	the side chain	1303:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	57	from	brains	1249:1254	arg1	PrPCs					1213:1217	human PrPCs	1207:1217	human PrPCs	1207:1217	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	57	from	brains	1249:1254	arg1	anchors					1196:1202	GPI anchors	1192:1202	GPI anchors in human PrPCs from human and knock-in mouse brains, respectively	1192:1268	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	2	58	theme	glycosylphosphatidylinositol	318:345	arg1	anchor					353:358	The glycosylphosphatidylinositol (GPI) anchor	314:358	The glycosylphosphatidylinositol (GPI) anchor of PrPC	314:366	The glycosylphosphatidylinositol (GPI) anchor of PrPC is involved in prion disease pathogenesis, and especially sialic acid in a GPI side chain reportedly affects PrPC conversion.					
32321762	1	59	theme	scrapie	217:223	arg1	form					225:228	the aggregated scrapie form	202:228	the aggregated scrapie form of the prion protein (PrPSc)	202:257	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	6	60	theme	human	1207:1211	arg1	PrPCs					1213:1217	human PrPCs	1207:1217	human PrPCs	1207:1217	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	61	theme	ω	1132:1132	arg1	Ser231					1100:1105	Ser231	1100:1105	Ser231	1100:1105	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	61	theme	ω	1132:1132	arg1	site					1134:1137	the previously reported ω site	1108:1137	the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain	1108:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	61	theme	ω	1132:1132	arg1	PrPC					1157:1160	Syrian hamster PrPC	1142:1160	Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain	1142:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	3	62	dep	PrPC	580:583	arg1	determined					659:668	determined	659:668	has not been determined for any GPI-anchored protein	646:697	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	6	63	from	%	1187:1187	arg1	PrPCs					1213:1217	human PrPCs	1207:1217	human PrPCs	1207:1217	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	3	64	theme	acid	606:609	arg1	type					619:622	the sialic acid linkage type	595:622	the sialic acid linkage type in the GPI side chain	595:644	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	1	65	theme	prion	237:241	arg1	PrPSc					252:256	PrPSc	252:256	PrPSc	252:256	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	1	65	theme	prion	237:241	arg1	protein					243:249	the prion protein	233:249	the prion protein (PrPSc)	233:257	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	3	66	theme	side	635:638	arg1	chain					640:644	the GPI side chain	627:644	the GPI side chain	627:644	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	1	67	theme	neurodegenerative	148:164	arg1	diseases					113:120	Prion diseases	107:120	Prion diseases	107:120	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	1	67	theme	neurodegenerative	148:164	arg1	disorders					166:174	transmissible, lethal neurodegenerative disorders	126:174	transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC)	126:311	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	7	68	theme	N-acetylneuraminic	1440:1457	arg1	acid					1459:1462	N-acetylneuraminic acid	1440:1462	N-acetylneuraminic acid in PrPC's GPI side chain	1440:1487	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	4	69	theme	PrPC	746:749	arg1	structures					726:735	GPI glycan structures	715:735	GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene	715:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	0	70	theme	prion	92:96	arg1	protein					98:104	human prion protein	86:104	human prion protein	86:104	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	4	71	theme	knock-in	799:806	arg1	model					814:818	a knock-in mouse model	797:818	a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene	797:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	5	72	theme	human	946:950	arg1	PrPC					952:955	human PrPC	946:955	human PrPC from both biological sources	946:984	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	6	73	dep	PrPC	1157:1160	arg1	found					1165:1169	found	1165:1169	found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain	1165:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	4	74	theme	model	814:818	arg1	brains					787:792	brains	787:792	brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene	787:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	3	75	theme	GPI-anchored	678:689	arg1	protein					691:697	any GPI-anchored protein	674:697	any GPI-anchored protein	674:697	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	6	76	theme	hamster	1149:1155	arg1	PrPC					1157:1160	Syrian hamster PrPC	1142:1160	Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain	1142:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	7	77	theme	α2,6	1412:1415	arg1	linkage					1417:1423	α2,6 linkage	1412:1423	α2,6 linkage	1412:1423	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	6	78	theme	human	1066:1070	arg1	PrPC					1072:1075	human PrPC	1066:1075	human PrPC	1066:1075	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	5	79	theme	LC-electrospray	904:918	arg1	analysis					934:941	LC-electrospray ionization-MS analysis	904:941	LC-electrospray ionization-MS analysis of human PrPC from both biological sources	904:984	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	5	80	attach	attached	1046:1053	arg1	Gly229					1001:1006	Gly229	1001:1006	Gly229	1001:1006	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	5	80	attach	attached	1046:1053	arg2	GPI					1039:1041	GPI	1039:1041	GPI	1039:1041	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	5	80	attach	attached	1046:1053	arg1	site					1017:1020	the ω site	1011:1020	the ω site in PrPC to which GPI is attached	1011:1053	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	6	81	theme	anchors	1196:1202	arg1	%					1179:1179	∼41%	1176:1179	∼41%	1176:1179	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	81	theme	anchors	1196:1202	arg1	%					1187:1187	28%	1185:1187	28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively	1185:1268	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	81	theme	anchors	1196:1202	arg1	anchors					1196:1202	GPI anchors	1192:1202	GPI anchors in human PrPCs from human and knock-in mouse brains, respectively	1192:1268	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	82	from	PrPCs	1213:1217	arg1	%					1179:1179	∼41%	1176:1179	∼41%	1176:1179	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	82	from	PrPCs	1213:1217	arg1	%					1187:1187	28%	1185:1187	28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively	1185:1268	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	82	from	PrPCs	1213:1217	arg1	anchors					1196:1202	GPI anchors	1192:1202	GPI anchors in human PrPCs from human and knock-in mouse brains, respectively	1192:1268	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	1	83	theme	Prion	107:111	arg1	diseases					113:120	Prion diseases	107:120	Prion diseases	107:120	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	1	83	theme	Prion	107:111	arg1	disorders					166:174	transmissible, lethal neurodegenerative disorders	126:174	transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC)	126:311	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	4	84	theme	protein	845:851	arg1	gene					860:863	the mouse prion protein (Prnp) gene	829:863	the mouse prion protein (Prnp) gene	829:863	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	0	85	theme	unpredicted	51:61	arg1	site					78:81	an unpredicted GPI attachment site	48:81	an unpredicted GPI attachment site in human prion protein	48:104	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	0	85	theme	unpredicted	51:61	arg1	acid					23:26	sialic acid	16:26	sialic acid	16:26	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	1	86	theme	protein	298:304	arg1	conversion					265:274	conversion	265:274	conversion of the cellular prion protein (PrPC)	265:311	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	8	87	theme	glycan	1567:1572	arg1	structure					1574:1582	the GPI glycan structure	1559:1582	the GPI glycan structure	1559:1582	In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.					
32321762	4	88	theme	Prnp	854:857	arg1	gene					860:863	the mouse prion protein (Prnp) gene	829:863	the mouse prion protein (Prnp) gene	829:863	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	0	89	from	linkage	5:11	arg1	protein					98:104	human prion protein	86:104	human prion protein	86:104	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	0	90	theme	attachment	67:76	arg1	site					78:81	an unpredicted GPI attachment site	48:81	an unpredicted GPI attachment site in human prion protein	48:104	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	0	90	theme	attachment	67:76	arg1	acid					23:26	sialic acid	16:26	sialic acid	16:26	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	4	91	attach	isolated	751:758	arg1	brains					771:776	human brains	765:776	human brains	765:776	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	4	91	attach	isolated	751:758	arg1	brains					787:792	brains	787:792	brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene	787:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	4	91	attach	isolated	751:758	arg2	PrPC					746:749	human PrPC	740:749	human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene	740:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	7	92	theme	linkage-specific	1339:1354	arg1	method					1371:1376	a sialic acid linkage-specific alkylamidation method	1325:1376	a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage	1325:1423	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	5	93	theme	biological	967:976	arg1	sources					978:984	both biological sources	962:984	both biological sources	962:984	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	8	94	theme	PrPC	1593:1596	arg1	structure					1574:1582	the GPI glycan structure	1559:1582	the GPI glycan structure	1559:1582	In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.					
32321762	2	95	theme	side	447:450	arg1	chain					452:456	a GPI side chain	441:456	a GPI side chain	441:456	The glycosylphosphatidylinositol (GPI) anchor of PrPC is involved in prion disease pathogenesis, and especially sialic acid in a GPI side chain reportedly affects PrPC conversion.					
32321762	6	96	theme	mouse	1243:1247	arg1	brains					1249:1254	human and knock-in mouse brains	1224:1254	human and knock-in mouse brains	1224:1254	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	2	97	theme	disease	389:395	arg1	pathogenesis					397:408	prion disease pathogenesis	383:408	prion disease pathogenesis	383:408	The glycosylphosphatidylinositol (GPI) anchor of PrPC is involved in prion disease pathogenesis, and especially sialic acid in a GPI side chain reportedly affects PrPC conversion.					
32321762	1	98	dep	transmissible	126:138	arg1	lethal					141:146	lethal	141:146	lethal	141:146	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	0	99	theme	sialic	16:21	arg1	acid					23:26	sialic acid	16:26	sialic acid	16:26	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	6	100	contain	have	1271:1274	arg2	acid					1295:1298	N-acetylneuraminic acid	1276:1298	N-acetylneuraminic acid in the side chain	1276:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	100	contain	have	1271:1274	arg1	%					1179:1179	∼41%	1176:1179	∼41%	1176:1179	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	100	contain	have	1271:1274	arg1	%					1187:1187	28%	1185:1187	28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively	1185:1268	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	6	100	contain	have	1271:1274	arg1	anchors					1196:1202	GPI anchors	1192:1202	GPI anchors in human PrPCs from human and knock-in mouse brains, respectively	1192:1268	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	8	101	theme	ω-site	1613:1618	arg1	acid					1626:1629	the ω-site amino acid	1609:1629	the ω-site amino acid for GPI attachment	1609:1648	In summary, we report the GPI glycan structure of human PrPC, including the ω-site amino acid for GPI attachment and the sialic acid linkage type.					
32321762	7	102	theme	GPI	1474:1476	arg1	chain					1483:1487	PrPC's GPI side chain	1467:1487	PrPC's GPI side chain	1467:1487	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	4	103	theme	PRNP	893:896	arg1	gene					898:901	the human PRNP gene	883:901	the human PRNP gene	883:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	7	104	theme	acid	1334:1337	arg1	method					1371:1376	a sialic acid linkage-specific alkylamidation method	1325:1376	a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage	1325:1423	Using a sialic acid linkage-specific alkylamidation method to discriminate α2,3 linkage from α2,6 linkage, we found that N-acetylneuraminic acid in PrPC's GPI side chain is linked to galactose through an α2,3 linkage.					
32321762	3	105	theme	GPI	560:562	arg1	anchor					564:569	the GPI anchor	556:569	the GPI anchor	556:569	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	4	106	theme	mouse	833:837	arg1	gene					860:863	the mouse prion protein (Prnp) gene	829:863	the mouse prion protein (Prnp) gene	829:863	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	0	107	theme	GPI	33:35	arg1	anchor					37:42	a GPI anchor	31:42	a GPI anchor	31:42	α2,3 linkage of sialic acid to a GPI anchor and an unpredicted GPI attachment site in human prion protein.					
32321762	2	108	theme	PrPC	477:480	arg1	conversion					482:491	PrPC conversion	477:491	PrPC conversion	477:491	The glycosylphosphatidylinositol (GPI) anchor of PrPC is involved in prion disease pathogenesis, and especially sialic acid in a GPI side chain reportedly affects PrPC conversion.					
32321762	4	109	theme	glycan	719:724	arg1	structures					726:735	GPI glycan structures	715:735	GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene	715:901	Here we report GPI glycan structures of human PrPC isolated from human brains and from brains of a knock-in mouse model in which the mouse prion protein (Prnp) gene was replaced with the human PRNP gene.					
32321762	1	110	theme	cellular	283:290	arg1	PrPC					307:310	PrPC	307:310	PrPC	307:310	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	1	110	theme	cellular	283:290	arg1	protein					298:304	cellular prion protein	283:304	the cellular prion protein (PrPC)	279:311	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	6	111	theme	N-acetylneuraminic	1276:1293	arg1	acid					1295:1298	N-acetylneuraminic acid	1276:1298	N-acetylneuraminic acid in the side chain	1276:1316	Gly229 in human PrPC does not correspond to Ser231, the previously reported ω site of Syrian hamster PrPC We found that ∼41% and 28% of GPI anchors in human PrPCs from human and knock-in mouse brains, respectively, have N-acetylneuraminic acid in the side chain.					
32321762	1	112	theme	aggregated	206:215	arg1	form					225:228	the aggregated scrapie form	202:228	the aggregated scrapie form of the prion protein (PrPSc)	202:257	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32321762	3	113	theme	linkage	611:617	arg1	type					619:622	the sialic acid linkage type	595:622	the sialic acid linkage type in the GPI side chain	595:644	Thus, it is important to define the location and structure of the GPI anchor in human PrPC Moreover, the sialic acid linkage type in the GPI side chain has not been determined for any GPI-anchored protein.					
32321762	5	114	from	sources	978:984	arg1	analysis					934:941	LC-electrospray ionization-MS analysis	904:941	LC-electrospray ionization-MS analysis of human PrPC from both biological sources	904:984	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	5	114	from	sources	978:984	arg1	PrPC					952:955	human PrPC	946:955	human PrPC from both biological sources	946:984	LC-electrospray ionization-MS analysis of human PrPC from both biological sources indicated that Gly229 is the ω site in PrPC to which GPI is attached.					
32321762	1	115	theme	form	225:228	arg1	accumulation					186:197	accumulation	186:197	accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC)	186:311	Prion diseases are transmissible, lethal neurodegenerative disorders caused by accumulation of the aggregated scrapie form of the prion protein (PrPSc) after conversion of the cellular prion protein (PrPC).					
32794693	7	0	theme	walls	1533:1537	arg1	components					1508:1517	minor polysaccharide components	1487:1517	minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone	1487:1609	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	10	1	theme	chemical	2111:2118	arg1	values					2126:2131	chemical shift values	2111:2131	chemical shift values	2111:2131	In particular, the position of the arabinose residue in the arabinoxylan of the switchgrass sample was confidently assigned based on chemical shift values, which are highly sensitive to local chemical environments.					
32794693	8	2	theme	destarched	1692:1701	arg1	residues					1722:1729	destarched, alcohol-insoluble residues	1692:1729	destarched, alcohol-insoluble residues of switchgrass and poplar	1692:1755	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	8	2	theme	destarched	1692:1701	arg1	poplar					1750:1755	poplar	1750:1755	poplar	1750:1755	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	8	2	theme	destarched	1692:1701	arg1	switchgrass					1734:1744	switchgrass	1734:1744	switchgrass	1734:1744	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	8	3	theme	NMR	1776:1778	arg1	profiling					1789:1797	two-dimensional NMR spectral profiling	1760:1797	two-dimensional NMR spectral profiling	1760:1797	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	1	4	theme	mass	283:286	arg1	MS					302:303	MS	302:303	MS	302:303	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	1	4	theme	mass	283:286	arg1	spectrometry					288:299	mass spectrometry	283:299	mass spectrometry (MS)	283:304	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	7	5	theme	plant	1522:1526	arg1	walls					1533:1537	plant cell walls	1522:1537	plant cell walls that are particularly difficult to assign by NMR correlation data alone	1522:1609	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	7	6	theme	structure	1465:1473	arg1	analysis					1475:1482	NMR structure analysis	1461:1482	NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone	1461:1609	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	12	7	theme	plant	2538:2542	arg1	polysaccharides					2554:2568	insoluble plant cell wall polysaccharides	2528:2568	insoluble plant cell wall polysaccharides	2528:2568	The concepts herein developed will thus facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.					
32794693	6	8	theme	polysaccharide	1291:1304	arg1	materials					1306:1314	insoluble polysaccharide materials	1281:1314	insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies	1281:1393	This information is of especial relevance to the structure analysis of insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies.					
32794693	7	9	theme	components	1508:1517	arg1	analysis					1475:1482	NMR structure analysis	1461:1482	NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone	1461:1609	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	11	10	theme	poplar	2294:2299	arg1	samples					2301:2307	the permethylated switchgrass and poplar samples	2260:2307	the permethylated switchgrass and poplar samples	2260:2307	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	10	11	theme	chemical	2170:2177	arg1	environments					2179:2190	local chemical environments	2164:2190	local chemical environments	2164:2190	In particular, the position of the arabinose residue in the arabinoxylan of the switchgrass sample was confidently assigned based on chemical shift values, which are highly sensitive to local chemical environments.					
32794693	0	12	theme	Polysaccharides	123:137	arg1	Characterization					77:92	Characterization	77:92	Characterization of Insoluble Plant Cell Wall Polysaccharides	77:137	Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.					
32794693	4	13	theme	analytical	936:945	arg1	MS					968:969	MS	968:969	MS	968:969	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	4	13	theme	analytical	936:945	arg1	techniques					947:956	analytical techniques	936:956	analytical techniques including MS and NMR	936:977	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	4	13	theme	analytical	936:945	arg1	NMR					975:977	NMR	975:977	NMR	975:977	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	11	14	theme	relative	2324:2331	arg1	analysis					2346:2353	facile relative quantitative analysis	2317:2353	facile relative quantitative analysis of their polysaccharide composition	2317:2389	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	10	15	theme	residue	2023:2029	arg1	position					1997:2004	the position	1993:2004	the position of the arabinose residue in the arabinoxylan of the switchgrass sample	1993:2075	In particular, the position of the arabinose residue in the arabinoxylan of the switchgrass sample was confidently assigned based on chemical shift values, which are highly sensitive to local chemical environments.					
32794693	6	16	theme	NMR	1377:1379	arg1	methodologies					1381:1393	solution-state NMR methodologies	1362:1393	solution-state NMR methodologies	1362:1393	This information is of especial relevance to the structure analysis of insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies.					
32794693	5	17	theme	chemical	1089:1096	arg1	data					1104:1107	chemical shift data	1089:1107	chemical shift data	1089:1107	In this work, NMR structure analysis of 10 permethylated polysaccharide standards was undertaken to generate chemical shift data providing insights into spectral changes that result from permethylation of polysaccharide residues.					
32794693	1	18	theme	tools	235:239	arg1	chromatography					267:280	gas and liquid chromatography	252:280	chromatography	267:280	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	1	18	theme	tools	235:239	arg1	spectrometry					288:299	mass spectrometry	283:299	mass spectrometry (MS)	283:304	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	1	18	theme	tools	235:239	arg1	spectroscopy					344:355	nuclear magnetic resonance (NMR) spectroscopy	311:355	nuclear magnetic resonance (NMR) spectroscopy	311:355	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	1	18	theme	tools	235:239	arg1	variety					213:219	a variety	211:219	a variety	211:219	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	1	18	theme	tools	235:239	arg1	tools					235:239	analytical tools	224:239	analytical tools	224:239	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	11	19	theme	facile	2317:2322	arg1	analysis					2346:2353	facile relative quantitative analysis	2317:2353	facile relative quantitative analysis of their polysaccharide composition	2317:2389	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	5	20	theme	polysaccharide	1037:1050	arg1	standards					1052:1060	10 permethylated polysaccharide standards	1020:1060	10 permethylated polysaccharide standards	1020:1060	In this work, NMR structure analysis of 10 permethylated polysaccharide standards was undertaken to generate chemical shift data providing insights into spectral changes that result from permethylation of polysaccharide residues.					
32794693	3	21	theme	varying	587:593	arg1	solubilities					595:606	varying solubilities	587:606	varying solubilities	587:606	However, cell walls contain fractions of varying solubilities, and a few techniques are available that can analyze all fractions simultaneously.					
32794693	7	22	theme	chemical	1416:1423	arg1	vital					1451:1455	vital	1451:1455	vital	1451:1455	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	7	22	theme	chemical	1416:1423	arg1	data					1431:1434	The preassigned NMR chemical shift data	1396:1434	The preassigned NMR chemical shift data	1396:1434	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	1	23	theme	cell	146:149	arg1	analysis					171:178	Plant cell wall polysaccharide analysis	140:178	Plant cell wall polysaccharide analysis	140:178	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	2	24	theme	complex	508:514	arg1	matrices					516:523	the complex matrices	504:523	the complex matrices of plant cell walls	504:543	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	7	25	theme	preassigned	1400:1410	arg1	vital					1451:1455	vital	1451:1455	vital	1451:1455	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	7	25	theme	preassigned	1400:1410	arg1	data					1431:1434	The preassigned NMR chemical shift data	1396:1434	The preassigned NMR chemical shift data	1396:1434	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	0	26	theme	Data	68:71	arg1	Utility					15:21	the Utility	11:21	the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides	11:137	Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.					
32794693	5	27	theme	spectral	1133:1140	arg1	changes					1142:1148	spectral changes	1133:1148	spectral changes that result from permethylation of polysaccharide residues	1133:1207	In this work, NMR structure analysis of 10 permethylated polysaccharide standards was undertaken to generate chemical shift data providing insights into spectral changes that result from permethylation of polysaccharide residues.					
32794693	1	28	theme	polysaccharide	156:169	arg1	analysis					171:178	Plant cell wall polysaccharide analysis	140:178	Plant cell wall polysaccharide analysis	140:178	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	2	29	theme	cell	534:537	arg1	walls					539:543	plant cell walls	528:543	plant cell walls	528:543	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	6	30	theme	especial	1233:1240	arg1	relevance					1242:1250	especial relevance	1233:1250	especial relevance	1233:1250	This information is of especial relevance to the structure analysis of insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies.					
32794693	10	31	theme	switchgrass	2058:2068	arg1	sample					2070:2075	the switchgrass sample	2054:2075	the switchgrass sample	2054:2075	In particular, the position of the arabinose residue in the arabinoxylan of the switchgrass sample was confidently assigned based on chemical shift values, which are highly sensitive to local chemical environments.					
32794693	0	32	theme	Insoluble	97:105	arg1	Polysaccharides					123:137	Insoluble Plant Cell Wall Polysaccharides	97:137	Insoluble Plant Cell Wall Polysaccharides	97:137	Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.					
32794693	9	33	theme	major	1840:1844	arg1	components					1861:1870	the major polysaccharide components	1836:1870	the major polysaccharide components	1836:1870	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	12	34	theme	cell	2544:2547	arg1	polysaccharides					2554:2568	insoluble plant cell wall polysaccharides	2528:2568	insoluble plant cell wall polysaccharides	2528:2568	The concepts herein developed will thus facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.					
32794693	4	35	theme	polysaccharide	793:806	arg1	fractions					808:816	both soluble and insoluble polysaccharide fractions	766:816	both soluble and insoluble polysaccharide fractions of plant cell walls	766:836	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	0	36	theme	Cell	113:116	arg1	Polysaccharides					123:137	Insoluble Plant Cell Wall Polysaccharides	97:137	Insoluble Plant Cell Wall Polysaccharides	97:137	Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.					
32794693	2	37	theme	many	451:454	arg1	structures					479:488	the many complex polysaccharide structures	447:488	the many complex polysaccharide structures	447:488	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	11	38	theme	NMR	2245:2247	arg1	spectra					2249:2255	the 1H NMR spectra	2238:2255	the 1H NMR spectra of the permethylated switchgrass and poplar samples	2238:2307	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	11	39	theme	permethylated	2264:2276	arg1	samples					2301:2307	the permethylated switchgrass and poplar samples	2260:2307	the permethylated switchgrass and poplar samples	2260:2307	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	2	40	theme	complementary	380:392	arg1	data					394:397	complementary data	380:397	complementary data	380:397	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	5	41	theme	residues	1200:1207	arg1	permethylation					1167:1180	permethylation	1167:1180	permethylation of polysaccharide residues	1167:1207	In this work, NMR structure analysis of 10 permethylated polysaccharide standards was undertaken to generate chemical shift data providing insights into spectral changes that result from permethylation of polysaccharide residues.					
32794693	4	42	from	dissolution	751:761	arg1	chloroform					866:875	chloroform	866:875	chloroform	866:875	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	4	42	from	dissolution	751:761	arg1	acetonitrile					880:891	acetonitrile	880:891	acetonitrile	880:891	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	4	42	from	dissolution	751:761	arg1	solvents					849:856	organic solvents	841:856	organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR	841:977	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	12	43	theme	insoluble	2528:2536	arg1	polysaccharides					2554:2568	insoluble plant cell wall polysaccharides	2528:2568	insoluble plant cell wall polysaccharides	2528:2568	The concepts herein developed will thus facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.					
32794693	11	44	theme	wall	2436:2439	arg1	material					2441:2448	the cell wall material	2427:2448	the cell wall material	2427:2448	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	2	45	theme	structural	423:432	arg1	proposals					434:442	confident structural proposals	413:442	confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls	413:543	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	7	46	theme	correlation	1588:1598	arg1	data					1600:1603	NMR correlation data	1584:1603	NMR correlation data alone	1584:1609	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	3	47	contain	contain	566:572	arg2	fractions					574:582	fractions	574:582	fractions of varying solubilities	574:606	However, cell walls contain fractions of varying solubilities, and a few techniques are available that can analyze all fractions simultaneously.					
32794693	3	47	contain	contain	566:572	arg1	walls					560:564	cell walls	555:564	cell walls	555:564	However, cell walls contain fractions of varying solubilities, and a few techniques are available that can analyze all fractions simultaneously.					
32794693	0	48	theme	Permethylated	26:38	arg1	Data					68:71	Permethylated Polysaccharide Solution NMR Data	26:71	Permethylated Polysaccharide Solution NMR Data	26:71	Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.					
32794693	2	49	theme	polysaccharide	464:477	arg1	structures					479:488	the many complex polysaccharide structures	447:488	the many complex polysaccharide structures	447:488	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	8	50	theme	assigned	1621:1628	arg1	data					1645:1648	the assigned chemical shift data	1617:1648	the assigned chemical shift data	1617:1648	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	0	51	theme	Solution	55:62	arg1	Data					68:71	Permethylated Polysaccharide Solution NMR Data	26:71	Permethylated Polysaccharide Solution NMR Data	26:71	Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.					
32794693	1	52	theme	nuclear	311:317	arg1	NMR					339:341	NMR	339:341	NMR	339:341	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	1	52	theme	nuclear	311:317	arg1	resonance					328:336	nuclear magnetic resonance	311:336	nuclear magnetic resonance (NMR) spectroscopy	311:355	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	1	53	theme	resonance	328:336	arg1	spectroscopy					344:355	nuclear magnetic resonance (NMR) spectroscopy	311:355	nuclear magnetic resonance (NMR) spectroscopy	311:355	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	9	54	link	3-linked	1912:1919	arg1	switchgrass					1935:1945	switchgrass	1935:1945	switchgrass	1935:1945	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	9	54	link	3-linked	1912:1919	arg1	arabinoxylan					1921:1932	3-linked arabinoxylan	1912:1932	<5% 3-linked arabinoxylan (switchgrass)	1908:1946	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	5	55	theme	permethylated	1023:1035	arg1	standards					1052:1060	10 permethylated polysaccharide standards	1020:1060	10 permethylated polysaccharide standards	1020:1060	In this work, NMR structure analysis of 10 permethylated polysaccharide standards was undertaken to generate chemical shift data providing insights into spectral changes that result from permethylation of polysaccharide residues.					
32794693	11	56	theme	polysaccharide	2364:2377	arg1	composition					2379:2389	their polysaccharide composition	2358:2389	their polysaccharide composition	2358:2389	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	8	57	theme	shift	1639:1643	arg1	data					1645:1648	the assigned chemical shift data	1617:1648	the assigned chemical shift data	1617:1648	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	9	58	theme	3-linked	1912:1919	arg1	switchgrass					1935:1945	switchgrass	1935:1945	switchgrass	1935:1945	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	9	58	theme	3-linked	1912:1919	arg1	arabinoxylan					1921:1932	3-linked arabinoxylan	1912:1932	<5% 3-linked arabinoxylan (switchgrass)	1908:1946	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	4	59	theme	cell	827:830	arg1	walls					832:836	plant cell walls	821:836	plant cell walls	821:836	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	8	60	theme	residues	1722:1729	arg1	samples					1681:1687	the permethylated samples	1663:1687	the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar	1663:1755	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	1	61	theme	gas	252:254	arg1	chromatography					267:280	gas and liquid chromatography	252:280	chromatography	267:280	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	11	62	theme	high	2210:2213	arg1	resolution					2215:2224	the high resolution	2206:2224	the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples	2206:2307	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	5	63	theme	structure	998:1006	arg1	analysis					1008:1015	NMR structure analysis	994:1015	NMR structure analysis of 10 permethylated polysaccharide standards	994:1060	In this work, NMR structure analysis of 10 permethylated polysaccharide standards was undertaken to generate chemical shift data providing insights into spectral changes that result from permethylation of polysaccharide residues.					
32794693	8	64	theme	switchgrass	1734:1744	arg1	residues					1722:1729	destarched, alcohol-insoluble residues	1692:1729	destarched, alcohol-insoluble residues of switchgrass and poplar	1692:1755	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	8	64	theme	switchgrass	1734:1744	arg1	poplar					1750:1755	poplar	1750:1755	poplar	1750:1755	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	8	64	theme	switchgrass	1734:1744	arg1	switchgrass					1734:1744	switchgrass	1734:1744	switchgrass	1734:1744	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	1	65	theme	liquid	260:265	arg1	chromatography					267:280	gas and liquid chromatography	252:280	chromatography	267:280	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	7	66	theme	cell	1528:1531	arg1	walls					1533:1537	plant cell walls	1522:1537	plant cell walls that are particularly difficult to assign by NMR correlation data alone	1522:1609	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	10	67	theme	shift	2120:2124	arg1	values					2126:2131	chemical shift values	2111:2131	chemical shift values	2111:2131	In particular, the position of the arabinose residue in the arabinoxylan of the switchgrass sample was confidently assigned based on chemical shift values, which are highly sensitive to local chemical environments.					
32794693	8	68	theme	permethylated	1667:1679	arg1	samples					1681:1687	the permethylated samples	1663:1687	the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar	1663:1755	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	9	69	dep	components	1861:1870	arg1	addition					1824:1831	addition	1824:1831	addition	1824:1831	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	8	70	theme	poplar	1750:1755	arg1	residues					1722:1729	destarched, alcohol-insoluble residues	1692:1729	destarched, alcohol-insoluble residues of switchgrass and poplar	1692:1755	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	8	70	theme	poplar	1750:1755	arg1	poplar					1750:1755	poplar	1750:1755	poplar	1750:1755	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	8	70	theme	poplar	1750:1755	arg1	switchgrass					1734:1744	switchgrass	1734:1744	switchgrass	1734:1744	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	7	71	theme	minor	1487:1491	arg1	components					1508:1517	minor polysaccharide components	1487:1517	minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone	1487:1609	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	11	72	theme	samples	2301:2307	arg1	spectra					2249:2255	the 1H NMR spectra	2238:2255	the 1H NMR spectra of the permethylated switchgrass and poplar samples	2238:2307	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	12	73	theme	wall	2549:2552	arg1	polysaccharides					2554:2568	insoluble plant cell wall polysaccharides	2528:2568	insoluble plant cell wall polysaccharides	2528:2568	The concepts herein developed will thus facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.					
32794693	6	74	theme	materials	1306:1314	arg1	analysis					1269:1276	the structure analysis	1255:1276	the structure analysis of insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies	1255:1393	This information is of especial relevance to the structure analysis of insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies.					
32794693	8	75	theme	two-dimensional	1760:1774	arg1	profiling					1789:1797	two-dimensional NMR spectral profiling	1760:1797	two-dimensional NMR spectral profiling	1760:1797	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	7	76	theme	polysaccharide	1493:1506	arg1	components					1508:1517	minor polysaccharide components	1487:1517	minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone	1487:1609	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	6	77	theme	insoluble	1281:1289	arg1	materials					1306:1314	insoluble polysaccharide materials	1281:1314	insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies	1281:1393	This information is of especial relevance to the structure analysis of insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies.					
32794693	8	78	theme	spectral	1780:1787	arg1	profiling					1789:1797	two-dimensional NMR spectral profiling	1760:1797	two-dimensional NMR spectral profiling	1760:1797	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	7	79	theme	NMR	1461:1463	arg1	analysis					1475:1482	NMR structure analysis	1461:1482	NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone	1461:1609	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	11	80	theme	quantitative	2333:2344	arg1	analysis					2346:2353	facile relative quantitative analysis	2317:2353	facile relative quantitative analysis of their polysaccharide composition	2317:2389	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	3	81	theme	cell	555:558	arg1	walls					560:564	cell walls	555:564	cell walls	555:564	However, cell walls contain fractions of varying solubilities, and a few techniques are available that can analyze all fractions simultaneously.					
32794693	1	82	theme	variety	213:219	arg1	utilization					196:206	the utilization	192:206	the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy	192:355	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	10	83	theme	local	2164:2168	arg1	environments					2179:2190	local chemical environments	2164:2190	local chemical environments	2164:2190	In particular, the position of the arabinose residue in the arabinoxylan of the switchgrass sample was confidently assigned based on chemical shift values, which are highly sensitive to local chemical environments.					
32794693	11	84	theme	switchgrass	2278:2288	arg1	samples					2301:2307	the permethylated switchgrass and poplar samples	2260:2307	the permethylated switchgrass and poplar samples	2260:2307	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	3	85	theme	solubilities	595:606	arg1	fractions					574:582	fractions	574:582	fractions of varying solubilities	574:606	However, cell walls contain fractions of varying solubilities, and a few techniques are available that can analyze all fractions simultaneously.					
32794693	1	86	theme	analytical	224:233	arg1	tools					235:239	analytical tools	224:239	analytical tools	224:239	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	12	87	theme	cell	2588:2591	arg1	components					2598:2607	minor cell wall components	2582:2607	minor cell wall components that are especially challenging to analyze with current methods	2582:2671	The concepts herein developed will thus facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.					
32794693	2	88	theme	walls	539:543	arg1	matrices					516:523	the complex matrices	504:523	the complex matrices of plant cell walls	504:543	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	6	89	theme	structure	1259:1267	arg1	analysis					1269:1276	the structure analysis	1255:1276	the structure analysis of insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies	1255:1393	This information is of especial relevance to the structure analysis of insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies.					
32794693	1	90	theme	magnetic	319:326	arg1	NMR					339:341	NMR	339:341	NMR	339:341	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	1	90	theme	magnetic	319:326	arg1	resonance					328:336	nuclear magnetic resonance	311:336	nuclear magnetic resonance (NMR) spectroscopy	311:355	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	10	91	theme	arabinose	2013:2021	arg1	residue					2023:2029	the arabinose residue	2009:2029	the arabinose residue	2009:2029	In particular, the position of the arabinose residue in the arabinoxylan of the switchgrass sample was confidently assigned based on chemical shift values, which are highly sensitive to local chemical environments.					
32794693	6	92	theme	solution-state	1362:1375	arg1	methodologies					1381:1393	solution-state NMR methodologies	1362:1393	solution-state NMR methodologies	1362:1393	This information is of especial relevance to the structure analysis of insoluble polysaccharide materials that otherwise are not easily investigated by solution-state NMR methodologies.					
32794693	5	93	theme	shift	1098:1102	arg1	data					1104:1107	chemical shift data	1089:1107	chemical shift data	1089:1107	In this work, NMR structure analysis of 10 permethylated polysaccharide standards was undertaken to generate chemical shift data providing insights into spectral changes that result from permethylation of polysaccharide residues.					
32794693	5	94	theme	standards	1052:1060	arg1	analysis					1008:1015	NMR structure analysis	994:1015	NMR structure analysis of 10 permethylated polysaccharide standards	994:1060	In this work, NMR structure analysis of 10 permethylated polysaccharide standards was undertaken to generate chemical shift data providing insights into spectral changes that result from permethylation of polysaccharide residues.					
32794693	3	95	theme	few	615:617	arg1	available					634:642	available	634:642	available	634:642	However, cell walls contain fractions of varying solubilities, and a few techniques are available that can analyze all fractions simultaneously.					
32794693	3	95	theme	few	615:617	arg1	techniques					619:628	a few techniques	613:628	a few techniques	613:628	However, cell walls contain fractions of varying solubilities, and a few techniques are available that can analyze all fractions simultaneously.					
32794693	9	96	dep	%	1954:1954	arg1	glucomannan					1956:1966	glucomannan	1956:1966	<2% glucomannan (poplar)	1952:1975	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	9	96	dep	%	1954:1954	arg1	poplar					1969:1974	poplar	1969:1974	poplar	1969:1974	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	1	97	theme	Plant	140:144	arg1	analysis					171:178	Plant cell wall polysaccharide analysis	140:178	Plant cell wall polysaccharide analysis	140:178	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	7	98	theme	shift	1425:1429	arg1	vital					1451:1455	vital	1451:1455	vital	1451:1455	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	7	98	theme	shift	1425:1429	arg1	data					1431:1434	The preassigned NMR chemical shift data	1396:1434	The preassigned NMR chemical shift data	1396:1434	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	12	99	theme	structure	2506:2514	arg1	analysis					2516:2523	NMR structure analysis	2502:2523	NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods	2502:2671	The concepts herein developed will thus facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.					
32794693	1	100	theme	wall	151:154	arg1	analysis					171:178	Plant cell wall polysaccharide analysis	140:178	Plant cell wall polysaccharide analysis	140:178	Plant cell wall polysaccharide analysis encompasses the utilization of a variety of analytical tools, including gas and liquid chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy.					
32794693	12	101	theme	minor	2582:2586	arg1	components					2598:2607	minor cell wall components	2582:2607	minor cell wall components that are especially challenging to analyze with current methods	2582:2671	The concepts herein developed will thus facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.					
32794693	7	102	theme	NMR	1412:1414	arg1	vital					1451:1455	vital	1451:1455	vital	1451:1455	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	7	102	theme	NMR	1412:1414	arg1	data					1431:1434	The preassigned NMR chemical shift data	1396:1434	The preassigned NMR chemical shift data	1396:1434	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	2	103	theme	plant	528:532	arg1	walls					539:543	plant cell walls	528:543	plant cell walls	528:543	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	11	104	theme	1H	2242:2243	arg1	spectra					2249:2255	the 1H NMR spectra	2238:2255	the 1H NMR spectra of the permethylated switchgrass and poplar samples	2238:2307	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	10	105	theme	sample	2070:2075	arg1	arabinoxylan					2038:2049	the arabinoxylan	2034:2049	the arabinoxylan of the switchgrass sample	2034:2075	In particular, the position of the arabinose residue in the arabinoxylan of the switchgrass sample was confidently assigned based on chemical shift values, which are highly sensitive to local chemical environments.					
32794693	0	106	theme	Plant	107:111	arg1	Polysaccharides					123:137	Insoluble Plant Cell Wall Polysaccharides	97:137	Insoluble Plant Cell Wall Polysaccharides	97:137	Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.					
32794693	10	107	from	position	1997:2004	arg1	arabinoxylan					2038:2049	the arabinoxylan	2034:2049	the arabinoxylan of the switchgrass sample	2034:2075	In particular, the position of the arabinose residue in the arabinoxylan of the switchgrass sample was confidently assigned based on chemical shift values, which are highly sensitive to local chemical environments.					
32794693	4	108	theme	techniques	947:956	arg1	MS					968:969	MS	968:969	MS	968:969	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	4	108	theme	techniques	947:956	arg1	number					926:931	a number	924:931	a number of analytical techniques including MS and NMR	924:977	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	4	108	theme	techniques	947:956	arg1	NMR					975:977	NMR	975:977	NMR	975:977	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	12	109	theme	current	2657:2663	arg1	methods					2665:2671	current methods	2657:2671	current methods	2657:2671	The concepts herein developed will thus facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.					
32794693	4	110	theme	soluble	771:777	arg1	fractions					808:816	both soluble and insoluble polysaccharide fractions	766:816	both soluble and insoluble polysaccharide fractions of plant cell walls	766:836	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	0	111	theme	Wall	118:121	arg1	Polysaccharides					123:137	Insoluble Plant Cell Wall Polysaccharides	97:137	Insoluble Plant Cell Wall Polysaccharides	97:137	Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.					
32794693	9	112	dep	%	1910:1910	arg1	switchgrass					1935:1945	switchgrass	1935:1945	switchgrass	1935:1945	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	9	112	dep	%	1910:1910	arg1	arabinoxylan					1921:1932	3-linked arabinoxylan	1912:1932	<5% 3-linked arabinoxylan (switchgrass)	1908:1946	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	9	113	theme	polysaccharide	1846:1859	arg1	components					1861:1870	the major polysaccharide components	1836:1870	the major polysaccharide components	1836:1870	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	11	114	theme	material	2441:2448	arg1	milligrams					2413:2422	only a few milligrams	2402:2422	only a few milligrams of the cell wall material	2402:2448	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	4	115	theme	insoluble	783:791	arg1	fractions					808:816	both soluble and insoluble polysaccharide fractions	766:816	both soluble and insoluble polysaccharide fractions of plant cell walls	766:836	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	11	116	theme	cell	2431:2434	arg1	material					2441:2448	the cell wall material	2427:2448	the cell wall material	2427:2448	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	4	117	theme	complete	742:749	arg1	dissolution					751:761	the complete dissolution	738:761	the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR	738:977	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	9	118	theme	minor	1877:1881	arg1	%					1910:1910	<5% 3-linked arabinoxylan (switchgrass)	1908:1946	<5% 3-linked arabinoxylan (switchgrass)	1908:1946	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	9	118	theme	minor	1877:1881	arg1	polysaccharides					1883:1897	two minor polysaccharides	1873:1897	two minor polysaccharides	1873:1897	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	9	118	theme	minor	1877:1881	arg1	%					1954:1954	<2% glucomannan (poplar)	1952:1975	<2% glucomannan (poplar)	1952:1975	Thus, we identified, in addition to the major polysaccharide components, two minor polysaccharides, namely, <5% 3-linked arabinoxylan (switchgrass) and <2% glucomannan (poplar).					
32794693	12	119	theme	NMR	2502:2504	arg1	analysis					2516:2523	NMR structure analysis	2502:2523	NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods	2502:2671	The concepts herein developed will thus facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.					
32794693	2	120	theme	confident	413:421	arg1	proposals					434:442	confident structural proposals	413:442	confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls	413:543	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	0	121	theme	Polysaccharide	40:53	arg1	Data					68:71	Permethylated Polysaccharide Solution NMR Data	26:71	Permethylated Polysaccharide Solution NMR Data	26:71	Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.					
32794693	2	122	theme	structures	479:488	arg1	proposals					434:442	confident structural proposals	413:442	confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls	413:543	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	8	123	theme	chemical	1630:1637	arg1	data					1645:1648	the assigned chemical shift data	1617:1648	the assigned chemical shift data	1617:1648	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	4	124	theme	walls	832:836	arg1	fractions					808:816	both soluble and insoluble polysaccharide fractions	766:816	both soluble and insoluble polysaccharide fractions of plant cell walls	766:836	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	0	125	theme	NMR	64:66	arg1	Data					68:71	Permethylated Polysaccharide Solution NMR Data	26:71	Permethylated Polysaccharide Solution NMR Data	26:71	Evaluating the Utility of Permethylated Polysaccharide Solution NMR Data for Characterization of Insoluble Plant Cell Wall Polysaccharides.					
32794693	8	126	dep	destarched	1692:1701	arg1	alcohol-insoluble					1704:1720	alcohol-insoluble	1704:1720	alcohol-insoluble	1704:1720	With the assigned chemical shift data, we analyzed the permethylated samples of destarched, alcohol-insoluble residues of switchgrass and poplar by two-dimensional NMR spectral profiling.					
32794693	7	127	theme	NMR	1584:1586	arg1	data					1600:1603	NMR correlation data	1584:1603	NMR correlation data alone	1584:1609	The preassigned NMR chemical shift data is shown to be vital for NMR structure analysis of minor polysaccharide components of plant cell walls that are particularly difficult to assign by NMR correlation data alone.					
32794693	2	128	theme	complex	456:462	arg1	structures					479:488	the many complex polysaccharide structures	447:488	the many complex polysaccharide structures	447:488	These methods provide complementary data, which enable confident structural proposals of the many complex polysaccharide structures that exist in the complex matrices of plant cell walls.					
32794693	4	129	theme	organic	841:847	arg1	chloroform					866:875	chloroform	866:875	chloroform	866:875	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	4	129	theme	organic	841:847	arg1	acetonitrile					880:891	acetonitrile	880:891	acetonitrile	880:891	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	4	129	theme	organic	841:847	arg1	solvents					849:856	organic solvents	841:856	organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR	841:977	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	5	130	theme	polysaccharide	1185:1198	arg1	residues					1200:1207	polysaccharide residues	1185:1207	polysaccharide residues	1185:1207	In this work, NMR structure analysis of 10 permethylated polysaccharide standards was undertaken to generate chemical shift data providing insights into spectral changes that result from permethylation of polysaccharide residues.					
32794693	11	131	theme	composition	2379:2389	arg1	analysis					2346:2353	facile relative quantitative analysis	2317:2353	facile relative quantitative analysis of their polysaccharide composition	2317:2389	Furthermore, the high resolution afforded by the 1H NMR spectra of the permethylated switchgrass and poplar samples allowed facile relative quantitative analysis of their polysaccharide composition, utilizing only a few milligrams of the cell wall material.					
32794693	4	132	theme	fractions	808:816	arg1	dissolution					751:761	the complete dissolution	738:761	the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR	738:977	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
32794693	5	133	theme	NMR	994:996	arg1	analysis					1008:1015	NMR structure analysis	994:1015	NMR structure analysis of 10 permethylated polysaccharide standards	994:1060	In this work, NMR structure analysis of 10 permethylated polysaccharide standards was undertaken to generate chemical shift data providing insights into spectral changes that result from permethylation of polysaccharide residues.					
32794693	12	134	theme	wall	2593:2596	arg1	components					2598:2607	minor cell wall components	2582:2607	minor cell wall components that are especially challenging to analyze with current methods	2582:2671	The concepts herein developed will thus facilitate NMR structure analysis of insoluble plant cell wall polysaccharides, more so of minor cell wall components that are especially challenging to analyze with current methods.					
32794693	4	135	theme	plant	821:825	arg1	walls					832:836	plant cell walls	821:836	plant cell walls	821:836	We have discovered that permethylation affords the complete dissolution of both soluble and insoluble polysaccharide fractions of plant cell walls in organic solvents such as chloroform or acetonitrile, which can then be analyzed by a number of analytical techniques including MS and NMR.					
34248085	6	0	theme	molecular	438:446	arg1	size					448:451	a molecular size	436:451	a molecular size of 5.9 × 103 kDa	436:468	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	1	theme	kDa	466:468	arg1	size					448:451	a molecular size	436:451	a molecular size of 5.9 × 103 kDa	436:468	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	5	2	theme	Chroococcus	409:419	arg1	sp					421:422	Chroococcus sp	409:422	Chroococcus sp	409:422	Purified EPS from Chroococcus sp.					
34248085	8	3	theme	molecular	819:827	arg1	size					829:832	molecular size	819:832	molecular size	819:832	However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS.					
34248085	6	4	theme	glucuronic	562:571	arg1	acid					573:576	glucuronic acid	562:576	glucuronic acid	562:576	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	7	5	theme	NaCl	685:688	arg1	concentration					690:702	the NaCl concentration	681:702	the NaCl concentration in the medium	681:716	The EPS content significantly increased when the NaCl concentration in the medium was increased from 1.7 to 100 mM.					
34248085	11	6	theme	EPS	1119:1121	arg1	production					1123:1132	EPS production	1119:1132	EPS production in this organism	1119:1149	These results suggest that the Wzy-dependent pathway is potentially involved in EPS production in this organism.					
34248085	11	7	theme	Wzy-dependent	1070:1082	arg1	pathway					1084:1090	the Wzy-dependent pathway	1066:1090	the Wzy-dependent pathway	1066:1090	These results suggest that the Wzy-dependent pathway is potentially involved in EPS production in this organism.					
34248085	9	8	theme	sp	992:993	arg1	genome					970:975	the genome	966:975	the genome of Chroococcus sp	966:993	The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp.					
34248085	11	9	from	production	1123:1132	arg1	organism					1142:1149	this organism	1137:1149	this organism	1137:1149	These results suggest that the Wzy-dependent pathway is potentially involved in EPS production in this organism.					
34248085	6	10	contain	had	432:434	arg2	size					448:451	a molecular size	436:451	a molecular size of 5.9 × 103 kDa	436:468	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	10	contain	had	432:434	arg1	FPU101					425:430	FPU101	425:430	FPU101	425:430	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	2	11	theme	unicellular	169:179	arg1	cyanobacterium					181:194	A unicellular cyanobacterium	167:194	A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS)	167:250	A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated.					
34248085	9	12	theme	Chroococcus	980:990	arg1	sp					992:993	Chroococcus sp	980:993	Chroococcus sp	980:993	The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp.					
34248085	8	13	theme	high	761:764	arg1	concentrations					771:784	high NaCl concentrations	761:784	high NaCl concentrations	761:784	However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS.					
34248085	8	14	theme	chemical	837:844	arg1	composition					846:856	chemical composition	837:856	chemical composition	837:856	However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS.					
34248085	0	15	theme	Physiological	0:12	arg1	analysis					26:33	Physiological and genomic analysis	0:33	Physiological and genomic analysis of newly-isolated polysaccharide	0:66	Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp.					
34248085	4	16	theme	m-2	382:384	arg1	s-1					386:388	10 and 80 μmol m-2 s-1	367:388	10 and 80 μmol m-2 s-1	367:388	FPU101, grew between 20 and 30°C and at light intensities between 10 and 80 μmol m-2 s-1.					
34248085	4	17	theme	μmol	377:380	arg1	s-1					386:388	10 and 80 μmol m-2 s-1	367:388	10 and 80 μmol m-2 s-1	367:388	FPU101, grew between 20 and 30°C and at light intensities between 10 and 80 μmol m-2 s-1.					
34248085	1	18	theme	chemical	123:130	arg1	analysis					132:139	chemical analysis	123:139	chemical analysis	123:139	FPU101 and chemical analysis of the exopolysaccharide.					
34248085	0	19	theme	genomic	18:24	arg1	analysis					26:33	Physiological and genomic analysis	0:33	Physiological and genomic analysis of newly-isolated polysaccharide	0:66	Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp.					
34248085	7	20	from	concentration	690:702	arg1	medium					711:716	the medium	707:716	the medium	707:716	The EPS content significantly increased when the NaCl concentration in the medium was increased from 1.7 to 100 mM.					
34248085	8	21	theme	EPS	865:867	arg1	size					829:832	molecular size	819:832	molecular size	819:832	However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS.					
34248085	8	21	theme	EPS	865:867	arg1	composition					846:856	chemical composition	837:856	chemical composition	837:856	However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS.					
34248085	0	22	theme	newly-isolated	38:51	arg1	polysaccharide					53:66	newly-isolated polysaccharide	38:66	newly-isolated polysaccharide	38:66	Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp.					
34248085	3	23	theme	Chroococcus	285:295	arg1	sp					297:298	Chroococcus sp	285:298	Chroococcus sp	285:298	The isolate, named Chroococcus sp.					
34248085	2	24	theme	large	212:216	arg1	exopolysaccharide					228:244	exopolysaccharide	228:244	exopolysaccharide (EPS)	228:250	A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated.					
34248085	2	24	theme	large	212:216	arg1	amount					218:223	a large amount	210:223	a large amount of exopolysaccharide (EPS)	210:250	A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated.					
34248085	2	24	theme	large	212:216	arg1	EPS					247:249	EPS	247:249	EPS	247:249	A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated.					
34248085	6	25	from	ratio	589:593	arg1	glucose					530:536	glucose	530:536	glucose	530:536	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	25	from	ratio	589:593	arg1	acid					573:576	glucuronic acid	562:576	glucuronic acid	562:576	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	25	from	ratio	589:593	arg1	fucose					505:510	fucose	505:510	fucose	505:510	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	25	from	ratio	589:593	arg1	xylose					513:518	xylose	513:518	xylose	513:518	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	25	from	ratio	589:593	arg1	acid					552:555	galacturonic acid	539:555	galacturonic acid	539:555	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	25	from	ratio	589:593	arg1	galactose					484:492	galactose	484:492	galactose	484:492	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	25	from	ratio	589:593	arg1	rhamnose					495:502	rhamnose	495:502	rhamnose	495:502	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	25	from	ratio	589:593	arg1	mannose					521:527	mannose	521:527	mannose	521:527	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	26	theme	molar	583:587	arg1	ratio					589:593	a molar ratio	581:593	a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7	581:633	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	27	theme	103	462:464	arg1	×					460:460	×	460:460	×	460:460	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	4	28	theme	light	341:345	arg1	intensities					347:357	light intensities	341:357	light intensities between 10 and 80 μmol m-2 s-1	341:388	FPU101, grew between 20 and 30°C and at light intensities between 10 and 80 μmol m-2 s-1.					
34248085	0	29	theme	polysaccharide	53:66	arg1	analysis					26:33	Physiological and genomic analysis	0:33	Physiological and genomic analysis of newly-isolated polysaccharide	0:66	Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp.					
34248085	6	30	theme	5.9	456:458	arg1	×					460:460	×	460:460	×	460:460	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	7	31	theme	EPS	640:642	arg1	content					644:650	The EPS content	636:650	The EPS content	636:650	The EPS content significantly increased when the NaCl concentration in the medium was increased from 1.7 to 100 mM.					
34248085	6	32	theme	17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7	598:633	arg1	ratio					589:593	a molar ratio	581:593	a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7	581:633	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	8	33	dep	size	829:832	arg1	the					815:817	the	815:817	the	815:817	However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS.					
34248085	1	34	theme	exopolysaccharide	148:164	arg1	FPU101					112:117	FPU101	112:117	FPU101	112:117	FPU101 and chemical analysis of the exopolysaccharide.					
34248085	1	34	theme	exopolysaccharide	148:164	arg1	analysis					132:139	chemical analysis	123:139	chemical analysis	123:139	FPU101 and chemical analysis of the exopolysaccharide.					
34248085	8	35	theme	NaCl	766:769	arg1	concentrations					771:784	high NaCl concentrations	761:784	high NaCl concentrations	761:784	However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS.					
34248085	2	36	theme	exopolysaccharide	228:244	arg1	exopolysaccharide					228:244	exopolysaccharide	228:244	exopolysaccharide (EPS)	228:250	A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated.					
34248085	2	36	theme	exopolysaccharide	228:244	arg1	amount					218:223	a large amount	210:223	a large amount of exopolysaccharide (EPS)	210:250	A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated.					
34248085	2	36	theme	exopolysaccharide	228:244	arg1	EPS					247:249	EPS	247:249	EPS	247:249	A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated.					
34248085	9	37	dep	genes	874:878	arg1	wzy					900:902	wzy	900:902	wzy	900:902	The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp.					
34248085	9	37	dep	genes	874:878	arg1	wzb					885:887	wzb	885:887	wzb	885:887	The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp.					
34248085	9	37	dep	genes	874:878	arg1	wzc					890:892	wzc	890:892	wzc	890:892	The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp.					
34248085	9	37	dep	genes	874:878	arg1	wzz					909:911	wzz	909:911	wzz	909:911	The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp.					
34248085	9	37	dep	genes	874:878	arg1	wza					880:882	wza	880:882	wza	880:882	The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp.					
34248085	9	37	dep	genes	874:878	arg1	wzx					895:897	wzx	895:897	wzx	895:897	The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp.					
34248085	9	38	theme	EPS	934:936	arg1	synthesis					938:946	EPS synthesis	934:946	EPS synthesis	934:946	The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp.					
34248085	0	39	theme	Chroococcus	96:106	arg1	sp					108:109	cyanobacterium Chroococcus sp	81:109	cyanobacterium Chroococcus sp	81:109	Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp.					
34248085	6	40	contain	contained	474:482	arg2	acid					573:576	glucuronic acid	562:576	glucuronic acid	562:576	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	40	contain	contained	474:482	arg2	galactose					484:492	galactose	484:492	galactose	484:492	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	40	contain	contained	474:482	arg2	rhamnose					495:502	rhamnose	495:502	rhamnose	495:502	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	40	contain	contained	474:482	arg2	mannose					521:527	mannose	521:527	mannose	521:527	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	40	contain	contained	474:482	arg2	fucose					505:510	fucose	505:510	fucose	505:510	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	40	contain	contained	474:482	arg1	FPU101					425:430	FPU101	425:430	FPU101	425:430	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	40	contain	contained	474:482	arg2	glucose					530:536	glucose	530:536	glucose	530:536	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	40	contain	contained	474:482	arg2	acid					552:555	galacturonic acid	539:555	galacturonic acid	539:555	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	6	40	contain	contained	474:482	arg2	xylose					513:518	xylose	513:518	xylose	513:518	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	0	41	theme	cyanobacterium	81:94	arg1	sp					108:109	cyanobacterium Chroococcus sp	81:109	cyanobacterium Chroococcus sp	81:109	Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp.					
34248085	6	42	theme	galacturonic	539:550	arg1	acid					552:555	galacturonic acid	539:555	galacturonic acid	539:555	FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7.					
34248085	7	43	dep	100	744:746	arg1	to					741:742	to	741:742	to	741:742	The EPS content significantly increased when the NaCl concentration in the medium was increased from 1.7 to 100 mM.					
34560950	0	0	theme	seed	79:82	arg1	gum					84:86	the seed gum	75:86	the seed gum from Chinese	75:99	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	1	1	theme	yield	265:269	arg1	terms					256:260	terms	256:260	terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability	256:425	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	2	2	theme	acid	532:535	arg1	treatments					537:546	acid treatments	532:546	acid treatments	532:546	The results showed that the yield of CQSG increased to 3.9% after water extraction at 80 °C. Alkali and acid treatments promoted the conversion of neutral sugars to the uronic acid branch.					
34560950	1	3	theme	quince	140:145	arg1	CQSG					157:160	CQSG	157:160	CQSG	157:160	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	3	theme	quince	140:145	arg1	gum					152:154	Chinese quince seed gum	132:154	Chinese quince seed gum (CQSG)	132:161	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	4	theme	seed	147:150	arg1	CQSG					157:160	CQSG	157:160	CQSG	157:160	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	4	theme	seed	147:150	arg1	gum					152:154	Chinese quince seed gum	132:154	Chinese quince seed gum (CQSG)	132:161	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	5	5	theme	CQSG	1148:1151	arg1	polysaccharides					1153:1167	CQSG polysaccharides	1148:1167	CQSG polysaccharides	1148:1167	This study provides theoretical support for the potential application of CQSG polysaccharides in the food and pharmaceutical industries.					
34560950	2	6	theme	CQSG	465:468	arg1	yield					456:460	the yield	452:460	the yield of CQSG	452:468	The results showed that the yield of CQSG increased to 3.9% after water extraction at 80 °C. Alkali and acid treatments promoted the conversion of neutral sugars to the uronic acid branch.					
34560950	0	7	from	Effects	0:6	arg1	properties					61:70	structural and functional properties	35:70	structural and functional properties of the seed gum from Chinese	35:99	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	2	8	theme	sugars	583:588	arg1	conversion					561:570	the conversion	557:570	the conversion of neutral sugars to the uronic acid branch	557:614	The results showed that the yield of CQSG increased to 3.9% after water extraction at 80 °C. Alkali and acid treatments promoted the conversion of neutral sugars to the uronic acid branch.					
34560950	0	9	dep	quince	101:106	arg1	sinensis					121:128	Chaenomeles sinensis	109:128	Chaenomeles sinensis	109:128	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	3	10	theme	extraction	751:760	arg1	temperature					762:772	the extraction temperature	747:772	the extraction temperature	747:772	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	5	11	theme	polysaccharides	1153:1167	arg1	application					1133:1143	the potential application	1119:1143	the potential application of CQSG polysaccharides in the food and pharmaceutical industries	1119:1209	This study provides theoretical support for the potential application of CQSG polysaccharides in the food and pharmaceutical industries.					
34560950	0	12	theme	gum	84:86	arg1	properties					61:70	structural and functional properties	35:70	structural and functional properties of the seed gum from Chinese	35:99	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	2	13	theme	80 °C.	514:519	arg1	Alkali					521:526	80 °C. Alkali	514:526	80 °C. Alkali	514:526	The results showed that the yield of CQSG increased to 3.9% after water extraction at 80 °C. Alkali and acid treatments promoted the conversion of neutral sugars to the uronic acid branch.					
34560950	2	14	theme	neutral	575:581	arg1	sugars					583:588	neutral sugars	575:588	neutral sugars	575:588	The results showed that the yield of CQSG increased to 3.9% after water extraction at 80 °C. Alkali and acid treatments promoted the conversion of neutral sugars to the uronic acid branch.					
34560950	3	15	theme	All	794:796	arg1	solutions					803:811	80 °C. All CQSG solutions	787:811	80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior	787:899	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	3	16	dep	component	689:697	arg1	observed					735:742	observed	735:742	was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior	731:899	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	1	17	from	80 °C	231:235	arg1	conditions					205:214	water-, alkali- and acid- conditions	179:214	water-, alkali- and acid- conditions at 25, 50, and 80 °C	179:235	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	18	theme	free	395:398	arg1	ability					419:425	free radical scavenging ability	395:425	free radical scavenging ability	395:425	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	18	theme	free	395:398	arg1	yield					265:269	yield	265:269	yield	265:269	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	4	19	theme	thermal	985:991	arg1	stability					993:1001	the worst thermal stability	975:1001	the worst thermal stability	975:1001	Compared to water-extracted samples, the alkali-extracted samples showed the worst thermal stability, while the acid-treated samples showed the worst emulsifying stability.					
34560950	3	20	theme	CQSG	798:801	arg1	solutions					803:811	80 °C. All CQSG solutions	787:811	80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior	787:899	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	3	21	theme	non-Newtonian	866:878	arg1	behavior					892:899	non-Newtonian rheological behavior	866:899	non-Newtonian rheological behavior	866:899	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	1	22	theme	molecular	300:308	arg1	yield					265:269	yield	265:269	yield	265:269	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	22	theme	molecular	300:308	arg1	distribution					310:321	molecular distribution	300:321	molecular distribution	300:321	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	4	23	theme	water-extracted	914:928	arg1	samples					930:936	water-extracted samples	914:936	water-extracted samples	914:936	Compared to water-extracted samples, the alkali-extracted samples showed the worst thermal stability, while the acid-treated samples showed the worst emulsifying stability.					
34560950	1	24	theme	radical	400:406	arg1	ability					419:425	free radical scavenging ability	395:425	free radical scavenging ability	395:425	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	24	theme	radical	400:406	arg1	yield					265:269	yield	265:269	yield	265:269	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	5	25	theme	food	1176:1179	arg1	industries					1200:1209	the food and pharmaceutical industries	1172:1209	the food and pharmaceutical industries	1172:1209	This study provides theoretical support for the potential application of CQSG polysaccharides in the food and pharmaceutical industries.					
34560950	5	26	theme	theoretical	1095:1105	arg1	support					1107:1113	theoretical support	1095:1113	theoretical support for the potential application of CQSG polysaccharides in the food and pharmaceutical industries	1095:1209	This study provides theoretical support for the potential application of CQSG polysaccharides in the food and pharmaceutical industries.					
34560950	4	27	theme	alkali-extracted	943:958	arg1	samples					960:966	the alkali-extracted samples	939:966	the alkali-extracted samples	939:966	Compared to water-extracted samples, the alkali-extracted samples showed the worst thermal stability, while the acid-treated samples showed the worst emulsifying stability.					
34560950	5	28	theme	potential	1123:1131	arg1	application					1133:1143	the potential application	1119:1143	the potential application of CQSG polysaccharides in the food and pharmaceutical industries	1119:1209	This study provides theoretical support for the potential application of CQSG polysaccharides in the food and pharmaceutical industries.					
34560950	1	29	theme	scavenging	408:417	arg1	ability					419:425	free radical scavenging ability	395:425	free radical scavenging ability	395:425	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	29	theme	scavenging	408:417	arg1	yield					265:269	yield	265:269	yield	265:269	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	3	30	theme	80 °C.	787:792	arg1	solutions					803:811	80 °C. All CQSG solutions	787:811	80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior	787:899	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	2	31	theme	uronic	597:602	arg1	branch					609:614	the uronic acid branch	593:614	the uronic acid branch	593:614	The results showed that the yield of CQSG increased to 3.9% after water extraction at 80 °C. Alkali and acid treatments promoted the conversion of neutral sugars to the uronic acid branch.					
34560950	0	32	theme	conditions	21:30	arg1	Effects					0:6	Effects	0:6	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese	0:99	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	0	33	theme	isolation	11:19	arg1	conditions					21:30	isolation conditions	11:30	isolation conditions	11:30	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	3	34	theme	xylose	663:668	arg1	component					689:697	the main component	680:697	the main component (35%-40%)	680:707	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	3	34	theme	xylose	663:668	arg1	chain					670:674	the xylose chain	659:674	the xylose chain	659:674	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	1	35	from	50	223:224	arg1	conditions					205:214	water-, alkali- and acid- conditions	179:214	water-, alkali- and acid- conditions at 25, 50, and 80 °C	179:235	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	4	36	theme	worst	1046:1050	arg1	stability					1064:1072	the worst emulsifying stability	1042:1072	the worst emulsifying stability	1042:1072	Compared to water-extracted samples, the alkali-extracted samples showed the worst thermal stability, while the acid-treated samples showed the worst emulsifying stability.					
34560950	0	37	theme	structural	35:44	arg1	properties					61:70	structural and functional properties	35:70	structural and functional properties of the seed gum from Chinese	35:99	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	4	38	theme	emulsifying	1052:1062	arg1	stability					1064:1072	the worst emulsifying stability	1042:1072	the worst emulsifying stability	1042:1072	Compared to water-extracted samples, the alkali-extracted samples showed the worst thermal stability, while the acid-treated samples showed the worst emulsifying stability.					
34560950	1	39	theme	thermal	324:330	arg1	gravimetry					332:341	thermal gravimetry	324:341	thermal gravimetry	324:341	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	39	theme	thermal	324:330	arg1	yield					265:269	yield	265:269	yield	265:269	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	40	theme	water-	179:184	arg1	conditions					205:214	water-, alkali- and acid- conditions	179:214	water-, alkali- and acid- conditions at 25, 50, and 80 °C	179:235	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	41	from	25	219:220	arg1	conditions					205:214	water-, alkali- and acid- conditions	179:214	water-, alkali- and acid- conditions at 25, 50, and 80 °C	179:235	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	3	42	theme	%	702:702	arg1	%					706:706	35%-40%	700:706	35%-40%	700:706	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	3	42	theme	%	702:702	arg1	component					689:697	the main component	680:697	the main component (35%-40%)	680:707	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	0	43	theme	functional	50:59	arg1	properties					61:70	structural and functional properties	35:70	structural and functional properties of the seed gum from Chinese	35:99	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	0	44	from	properties	61:70	arg1	Chinese					93:99	Chinese	93:99	Chinese	93:99	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	3	45	theme	extraction	635:644	arg1	temperature					646:656	the extraction temperature	631:656	the extraction temperature	631:656	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	1	46	theme	monosaccharide	272:285	arg1	yield					265:269	yield	265:269	yield	265:269	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	46	theme	monosaccharide	272:285	arg1	composition					287:297	monosaccharide composition	272:297	monosaccharide composition	272:297	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	3	47	theme	main	684:687	arg1	%					706:706	35%-40%	700:706	35%-40%	700:706	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	3	47	theme	main	684:687	arg1	component					689:697	the main component	680:697	the main component (35%-40%)	680:707	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	3	47	theme	main	684:687	arg1	chain					670:674	the xylose chain	659:674	the xylose chain	659:674	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	1	48	theme	emulsifying	344:354	arg1	stability					356:364	emulsifying stability	344:364	emulsifying stability	344:364	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	48	theme	emulsifying	344:354	arg1	yield					265:269	yield	265:269	yield	265:269	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	5	49	from	application	1133:1143	arg1	industries					1200:1209	the food and pharmaceutical industries	1172:1209	the food and pharmaceutical industries	1172:1209	This study provides theoretical support for the potential application of CQSG polysaccharides in the food and pharmaceutical industries.					
34560950	3	50	theme	isolation	835:843	arg1	conditions					845:854	these isolation conditions	829:854	these isolation conditions exhibited non-Newtonian rheological behavior	829:899	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	2	51	theme	water	494:498	arg1	extraction					500:509	water extraction	494:509	water extraction	494:509	The results showed that the yield of CQSG increased to 3.9% after water extraction at 80 °C. Alkali and acid treatments promoted the conversion of neutral sugars to the uronic acid branch.					
34560950	0	52	from	Chinese	93:99	arg1	properties					61:70	structural and functional properties	35:70	structural and functional properties of the seed gum from Chinese	35:99	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	0	52	from	Chinese	93:99	arg1	gum					84:86	the seed gum	75:86	the seed gum from Chinese	75:99	Effects of isolation conditions on structural and functional properties of the seed gum from Chinese quince (Chaenomeles sinensis).					
34560950	1	53	theme	alkali-	187:193	arg1	conditions					205:214	water-, alkali- and acid- conditions	179:214	water-, alkali- and acid- conditions at 25, 50, and 80 °C	179:235	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	4	54	theme	acid-treated	1014:1025	arg1	samples					1027:1033	the acid-treated samples	1010:1033	the acid-treated samples	1010:1033	Compared to water-extracted samples, the alkali-extracted samples showed the worst thermal stability, while the acid-treated samples showed the worst emulsifying stability.					
34560950	2	55	theme	acid	604:607	arg1	branch					609:614	the uronic acid branch	593:614	the uronic acid branch	593:614	The results showed that the yield of CQSG increased to 3.9% after water extraction at 80 °C. Alkali and acid treatments promoted the conversion of neutral sugars to the uronic acid branch.					
34560950	1	56	theme	rheological	367:377	arg1	properties					379:388	rheological properties	367:388	rheological properties	367:388	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	56	theme	rheological	367:377	arg1	yield					265:269	yield	265:269	yield	265:269	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	57	theme	acid-	199:203	arg1	conditions					205:214	water-, alkali- and acid- conditions	179:214	water-, alkali- and acid- conditions at 25, 50, and 80 °C	179:235	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	3	58	theme	rheological	880:890	arg1	behavior					892:899	non-Newtonian rheological behavior	866:899	non-Newtonian rheological behavior	866:899	Regardless of the extraction temperature, the xylose chain was the main component (35%-40%); however, a reduction was observed as the extraction temperature increased to 80 °C. All CQSG solutions extracted under these isolation conditions exhibited non-Newtonian rheological behavior.					
34560950	1	59	theme	Chinese	132:138	arg1	CQSG					157:160	CQSG	157:160	CQSG	157:160	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	1	59	theme	Chinese	132:138	arg1	gum					152:154	Chinese quince seed gum	132:154	Chinese quince seed gum (CQSG)	132:161	Chinese quince seed gum (CQSG) extracted under water-, alkali- and acid- conditions at 25, 50, and 80 °C, were evaluated in terms of yield, monosaccharide composition, molecular distribution, thermal gravimetry, emulsifying stability, rheological properties, and free radical scavenging ability.					
34560950	4	60	theme	worst	979:983	arg1	stability					993:1001	the worst thermal stability	975:1001	the worst thermal stability	975:1001	Compared to water-extracted samples, the alkali-extracted samples showed the worst thermal stability, while the acid-treated samples showed the worst emulsifying stability.					
34560950	5	61	theme	pharmaceutical	1185:1198	arg1	industries					1200:1209	the food and pharmaceutical industries	1172:1209	the food and pharmaceutical industries	1172:1209	This study provides theoretical support for the potential application of CQSG polysaccharides in the food and pharmaceutical industries.					
31972440	0	0	contain	containing	69:78	arg1	tablets					61:67	matrix tablets	54:67	matrix tablets containing psyllium powder in combination with grewia polysaccharides	54:137	Mechanical and release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides.					
31972440	0	0	contain	containing	69:78	arg2	powder					89:94	psyllium powder	80:94	psyllium powder	80:94	Mechanical and release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides.					
31972440	6	1	theme	mechanical	931:940	arg1	strength					942:949	good mechanical strength	926:949	good mechanical strength (108.33 N and 95.70 N, respectively)	926:986	The results show that GG and GDS produced tablets with good mechanical strength (108.33 N and 95.70 N, respectively) while psyllium produced softer tablets (7.13 N).					
31972440	1	2	theme	tablet	259:264	arg1	matrices					266:273	tablet matrices	259:273	tablet matrices containing binary mixtures of the polysaccharide with psyllium	259:336	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	0	3	theme	psyllium	80:87	arg1	powder					89:94	psyllium powder	80:94	psyllium powder	80:94	Mechanical and release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides.					
31972440	7	4	theme	grewia	1069:1074	arg1	polysaccharides					1076:1090	psyllium and grewia polysaccharides	1056:1090	psyllium and grewia polysaccharides	1056:1090	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	10	5	from	matrices	1714:1721	arg1	release					1696:1702	a more sustained drug release	1674:1702	a more sustained drug release from such matrices	1674:1721	When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.					
31972440	10	5	from	matrices	1714:1721	arg1	reduction					1660:1668	an effective reduction	1647:1668	an effective reduction	1647:1668	When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.					
31972440	8	6	theme	psyllium	1399:1406	arg1	matrices					1408:1415	the psyllium matrices	1395:1415	the psyllium matrices	1395:1415	The results also showed that GG and GDS reduced the dissolution rate and effectively eliminated the burst release of theophylline from the psyllium matrices at both pHs.					
31972440	1	7	theme	matrices	266:273	arg1	properties					245:254	the mechanical and release properties	218:254	properties	245:254	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	3	8	theme	binary	626:631	arg1	composites					633:642	binary composites	626:642	binary composites	626:642	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	3	9	theme	bulk	551:554	arg1	index					601:605	Carr's compressibility index	578:605	Carr's compressibility index of the powders and binary composites	578:642	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	3	9	theme	bulk	551:554	arg1	densities					567:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	1	10	from	effect	182:187	arg1	properties					245:254	the mechanical and release properties	218:254	properties	245:254	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	7	11	theme	mechanical	1150:1159	arg1	strength					1161:1168	the mechanical strength	1146:1168	the mechanical strength of the matrices	1146:1184	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	7	12	theme	psyllium	1056:1063	arg1	polysaccharides					1076:1090	psyllium and grewia polysaccharides	1056:1090	psyllium and grewia polysaccharides	1056:1090	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	1	13	theme	binary	286:291	arg1	mixtures					293:300	binary mixtures	286:300	binary mixtures of the polysaccharide with psyllium	286:336	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	0	14	theme	grewia	116:121	arg1	polysaccharides					123:137	grewia polysaccharides	116:137	grewia polysaccharides	116:137	Mechanical and release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides.					
31972440	7	15	contain	containing	1212:1221	arg2	psyllium					1223:1230	psyllium	1223:1230	psyllium alone	1223:1236	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	7	15	contain	containing	1212:1221	arg1	matrices					1203:1210	matrices	1203:1210	matrices containing psyllium alone as the matrix former	1203:1257	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	7	15	contain	containing	1212:1221	arg2	former					1252:1257	former	1252:1257	former	1252:1257	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	3	16	theme	true	545:548	arg1	index					601:605	Carr's compressibility index	578:605	Carr's compressibility index of the powders and binary composites	578:642	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	3	16	theme	true	545:548	arg1	densities					567:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	5	17	theme	phosphate	844:852	arg1	pH					862:863	pH 6.8	862:867	pH 6.8	862:867	The dissolution test was carried out in 0.1 M HCl (pH 1.2) and phosphate buffer (pH 6.8).					
31972440	5	17	theme	phosphate	844:852	arg1	buffer					854:859	phosphate buffer	844:859	phosphate buffer (pH 6.8)	844:868	The dissolution test was carried out in 0.1 M HCl (pH 1.2) and phosphate buffer (pH 6.8).					
31972440	6	18	with	tablets	913:919	arg1	strength					942:949	good mechanical strength	926:949	good mechanical strength (108.33 N and 95.70 N, respectively)	926:986	The results show that GG and GDS produced tablets with good mechanical strength (108.33 N and 95.70 N, respectively) while psyllium produced softer tablets (7.13 N).					
31972440	2	19	theme	model	529:533	arg1	drug					535:538	the model drug	525:538	the model drug	525:538	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	2	19	theme	model	529:533	arg1	theophylline					509:520	theophylline	509:520	theophylline	509:520	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	10	20	theme	grewia	1612:1617	arg1	polysaccharides					1619:1633	grewia polysaccharides	1612:1633	grewia polysaccharides	1612:1633	When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.					
31972440	10	21	theme	burst	1558:1562	arg1	release					1564:1570	the burst release	1554:1570	the burst release of drug from matrices such as psyllium	1554:1609	When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.					
31972440	4	22	from	matrices	752:759	arg1	hardness					706:713	hardness	706:713	hardness	706:713	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	22	from	matrices	752:759	arg1	release					735:741	drug release	730:741	drug release	730:741	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	22	from	matrices	752:759	arg1	properties					694:703	Tablet properties	687:703	Tablet properties (hardness, porosity, and drug release from the matrices)	687:760	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	22	from	matrices	752:759	arg1	porosity					716:723	porosity	716:723	porosity	716:723	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	23	dep	properties	694:703	arg1	hardness					706:713	hardness	706:713	hardness	706:713	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	23	dep	properties	694:703	arg1	release					735:741	drug release	730:741	drug release	730:741	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	23	dep	properties	694:703	arg1	properties					694:703	Tablet properties	687:703	Tablet properties (hardness, porosity, and drug release from the matrices)	687:760	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	23	dep	properties	694:703	arg1	porosity					716:723	porosity	716:723	porosity	716:723	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	10	24	theme	drug	1575:1578	arg1	release					1564:1570	the burst release	1554:1570	the burst release of drug from matrices such as psyllium	1554:1609	When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.					
31972440	9	25	with	diffusion	1513:1521	arg1	>					1530:1530	n > 0.45	1528:1535	n > 0.45	1528:1535	The matrices of GG or GDS and the binary mixtures conform to non-Fickian anomalous diffusion with n > 0.45.					
31972440	7	26	from	combination	1041:1051	arg1	matrices					1099:1106	the matrices	1095:1106	the matrices	1095:1106	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	8	27	from	matrices	1408:1415	arg1	release					1366:1372	the burst release	1356:1372	the burst release of theophylline from the psyllium matrices	1356:1415	The results also showed that GG and GDS reduced the dissolution rate and effectively eliminated the burst release of theophylline from the psyllium matrices at both pHs.					
31972440	9	28	theme	non-Fickian	1491:1501	arg1	diffusion					1513:1521	non-Fickian anomalous diffusion	1491:1521	non-Fickian anomalous diffusion with n > 0.45	1491:1535	The matrices of GG or GDS and the binary mixtures conform to non-Fickian anomalous diffusion with n > 0.45.					
31972440	4	29	theme	Tablet	687:692	arg1	hardness					706:713	hardness	706:713	hardness	706:713	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	29	theme	Tablet	687:692	arg1	properties					694:703	Tablet properties	687:703	Tablet properties (hardness, porosity, and drug release from the matrices)	687:760	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	29	theme	Tablet	687:692	arg1	release					735:741	drug release	730:741	drug release	730:741	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	29	theme	Tablet	687:692	arg1	porosity					716:723	porosity	716:723	porosity	716:723	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	7	30	theme	significant	1122:1132	arg1	increase					1134:1141	a significant increase	1120:1141	a significant increase in the mechanical strength of the matrices	1120:1184	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	6	31	theme	good	926:929	arg1	strength					942:949	good mechanical strength	926:949	good mechanical strength (108.33 N and 95.70 N, respectively)	926:986	The results show that GG and GDS produced tablets with good mechanical strength (108.33 N and 95.70 N, respectively) while psyllium produced softer tablets (7.13 N).					
31972440	1	32	with	mixtures	293:300	arg1	psyllium					329:336	psyllium	329:336	psyllium	329:336	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	9	33	theme	anomalous	1503:1511	arg1	diffusion					1513:1521	non-Fickian anomalous diffusion	1491:1521	non-Fickian anomalous diffusion with n > 0.45	1491:1535	The matrices of GG or GDS and the binary mixtures conform to non-Fickian anomalous diffusion with n > 0.45.					
31972440	1	34	theme	grewia	192:197	arg1	polysaccharides					199:213	grewia polysaccharides	192:213	grewia polysaccharides	192:213	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	0	35	with	combination	99:109	arg1	polysaccharides					123:137	grewia polysaccharides	116:137	grewia polysaccharides	116:137	Mechanical and release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides.					
31972440	2	36	dep	polysaccharides	360:374	arg1	GG					377:378	GG	377:378	GG	377:378	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	2	36	dep	polysaccharides	360:374	arg1	GDS					384:386	GDS	384:386	GDS	384:386	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	1	37	theme	polysaccharides	199:213	arg1	effect					182:187	the effect	178:187	the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium	178:336	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	0	38	theme	release	15:21	arg1	behaviour					23:31	release behaviour	15:31	release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides	15:137	Mechanical and release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides.					
31972440	7	39	from	increase	1134:1141	arg1	strength					1161:1168	the mechanical strength	1146:1168	the mechanical strength of the matrices	1146:1184	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	8	40	theme	dissolution	1312:1322	arg1	rate					1324:1327	the dissolution rate	1308:1327	the dissolution rate	1308:1327	The results also showed that GG and GDS reduced the dissolution rate and effectively eliminated the burst release of theophylline from the psyllium matrices at both pHs.					
31972440	0	41	theme	theophylline	36:47	arg1	behaviour					23:31	release behaviour	15:31	release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides	15:137	Mechanical and release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides.					
31972440	1	42	theme	polysaccharide	309:322	arg1	mixtures					293:300	binary mixtures	286:300	binary mixtures of the polysaccharide with psyllium	286:336	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	9	43	theme	n	1528:1528	arg1	>					1530:1530	n > 0.45	1528:1535	n > 0.45	1528:1535	The matrices of GG or GDS and the binary mixtures conform to non-Fickian anomalous diffusion with n > 0.45.					
31972440	6	44	theme	softer	1012:1017	arg1	tablets					1019:1025	softer tablets	1012:1025	softer tablets (7.13 N)	1012:1034	The results show that GG and GDS produced tablets with good mechanical strength (108.33 N and 95.70 N, respectively) while psyllium produced softer tablets (7.13 N).					
31972440	6	44	theme	softer	1012:1017	arg1	7.13 N					1028:1033	7.13 N	1028:1033	7.13 N	1028:1033	The results show that GG and GDS produced tablets with good mechanical strength (108.33 N and 95.70 N, respectively) while psyllium produced softer tablets (7.13 N).					
31972440	7	45	theme	polysaccharides	1076:1090	arg1	combination					1041:1051	The combination	1037:1051	The combination of psyllium and grewia polysaccharides in the matrices	1037:1106	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	2	46	theme	tablet	482:487	arg1	matrices					489:496	tablet matrices	482:496	tablet matrices containing theophylline as the model drug	482:538	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	5	47	theme	dissolution	785:795	arg1	test					797:800	The dissolution test	781:800	The dissolution test	781:800	The dissolution test was carried out in 0.1 M HCl (pH 1.2) and phosphate buffer (pH 6.8).					
31972440	3	48	theme	composites	633:642	arg1	index					601:605	Carr's compressibility index	578:605	Carr's compressibility index of the powders and binary composites	578:642	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	3	48	theme	composites	633:642	arg1	densities					567:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	10	49	theme	such	1709:1712	arg1	matrices					1714:1721	such matrices	1709:1721	such matrices	1709:1721	When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.					
31972440	2	50	contain	containing	498:507	arg2	drug					535:538	the model drug	525:538	the model drug	525:538	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	2	50	contain	containing	498:507	arg2	theophylline					509:520	theophylline	509:520	theophylline	509:520	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	2	50	contain	containing	498:507	arg1	matrices					489:496	tablet matrices	482:496	tablet matrices containing theophylline as the model drug	482:538	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	1	51	theme	mechanical	222:231	arg1	properties					245:254	the mechanical and release properties	218:254	properties	245:254	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	0	52	theme	matrix	54:59	arg1	tablets					61:67	matrix tablets	54:67	matrix tablets containing psyllium powder in combination with grewia polysaccharides	54:137	Mechanical and release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides.					
31972440	5	53	theme	0.1 M	821:825	arg1	pH					832:833	pH 1.2	832:837	pH 1.2	832:837	The dissolution test was carried out in 0.1 M HCl (pH 1.2) and phosphate buffer (pH 6.8).					
31972440	5	53	theme	0.1 M	821:825	arg1	HCl					827:829	0.1 M HCl	821:829	0.1 M HCl (pH 1.2)	821:838	The dissolution test was carried out in 0.1 M HCl (pH 1.2) and phosphate buffer (pH 6.8).					
31972440	0	54	from	tablets	61:67	arg1	behaviour					23:31	release behaviour	15:31	release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides	15:137	Mechanical and release behaviour of theophylline from matrix tablets containing psyllium powder in combination with grewia polysaccharides.					
31972440	2	55	with	polysaccharides	431:445	arg1	psyllium					452:459	psyllium	452:459	psyllium	452:459	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	2	56	theme	polysaccharides	360:374	arg1	grades					343:348	Two grades	339:348	Two grades of grewia polysaccharides (GG and GDS)	339:387	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	10	57	theme	effective	1650:1658	arg1	reduction					1660:1668	an effective reduction	1647:1668	an effective reduction	1647:1668	When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.					
31972440	9	58	theme	binary	1464:1469	arg1	mixtures					1471:1478	the binary mixtures	1460:1478	the binary mixtures	1460:1478	The matrices of GG or GDS and the binary mixtures conform to non-Fickian anomalous diffusion with n > 0.45.					
31972440	3	59	theme	compressibility	585:599	arg1	index					601:605	Carr's compressibility index	578:605	Carr's compressibility index of the powders and binary composites	578:642	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	3	59	theme	compressibility	585:599	arg1	densities					567:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	2	60	theme	grewia	353:358	arg1	polysaccharides					360:374	grewia polysaccharides	353:374	grewia polysaccharides (GG and GDS)	353:387	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	10	61	theme	drug	1691:1694	arg1	release					1696:1702	a more sustained drug release	1674:1702	a more sustained drug release from such matrices	1674:1721	When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.					
31972440	3	62	theme	powders	614:620	arg1	index					601:605	Carr's compressibility index	578:605	Carr's compressibility index of the powders and binary composites	578:642	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	3	62	theme	powders	614:620	arg1	densities					567:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	10	63	theme	sustained	1681:1689	arg1	release					1696:1702	a more sustained drug release	1674:1702	a more sustained drug release from such matrices	1674:1721	When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.					
31972440	4	64	theme	drug	730:733	arg1	release					735:741	drug release	730:741	drug release	730:741	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	4	64	theme	drug	730:733	arg1	properties					694:703	Tablet properties	687:703	Tablet properties (hardness, porosity, and drug release from the matrices)	687:760	Tablet properties (hardness, porosity, and drug release from the matrices) were investigated.					
31972440	3	65	theme	tapped	560:565	arg1	index					601:605	Carr's compressibility index	578:605	Carr's compressibility index of the powders and binary composites	578:642	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	3	65	theme	tapped	560:565	arg1	densities					567:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities	541:575	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	3	66	theme	tablet	667:672	arg1	compression					674:684	tablet compression	667:684	tablet compression	667:684	The true, bulk and tapped densities, Carr's compressibility index of the powders and binary composites were determined before tablet compression.					
31972440	1	67	contain	containing	275:284	arg1	matrices					266:273	tablet matrices	259:273	tablet matrices containing binary mixtures of the polysaccharide with psyllium	259:336	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	1	67	contain	containing	275:284	arg2	mixtures					293:300	binary mixtures	286:300	binary mixtures of the polysaccharide with psyllium	286:336	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	9	68	theme	GG	1446:1447	arg1	matrices					1434:1441	The matrices	1430:1441	The matrices of GG or GDS	1430:1454	The matrices of GG or GDS and the binary mixtures conform to non-Fickian anomalous diffusion with n > 0.45.					
31972440	9	68	theme	GG	1446:1447	arg1	mixtures					1471:1478	the binary mixtures	1460:1478	the binary mixtures	1460:1478	The matrices of GG or GDS and the binary mixtures conform to non-Fickian anomalous diffusion with n > 0.45.					
31972440	1	69	theme	release	237:243	arg1	properties					245:254	the mechanical and release properties	218:254	properties	245:254	This study was aimed at investigating the effect of grewia polysaccharides on the mechanical and release properties of tablet matrices containing binary mixtures of the polysaccharide with psyllium.					
31972440	8	70	theme	burst	1360:1364	arg1	release					1366:1372	the burst release	1356:1372	the burst release of theophylline from the psyllium matrices	1356:1415	The results also showed that GG and GDS reduced the dissolution rate and effectively eliminated the burst release of theophylline from the psyllium matrices at both pHs.					
31972440	2	71	theme	binary	408:413	arg1	mixtures					415:422	binary mixtures	408:422	binary mixtures of the polysaccharides with psyllium	408:459	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	8	72	theme	theophylline	1377:1388	arg1	release					1366:1372	the burst release	1356:1372	the burst release of theophylline from the psyllium matrices	1356:1415	The results also showed that GG and GDS reduced the dissolution rate and effectively eliminated the burst release of theophylline from the psyllium matrices at both pHs.					
31972440	10	73	from	matrices	1585:1592	arg1	release					1564:1570	the burst release	1554:1570	the burst release of drug from matrices such as psyllium	1554:1609	When overcoming the burst release of drug from matrices such as psyllium, grewia polysaccharides may provide an effective reduction and a more sustained drug release from such matrices.					
31972440	7	74	theme	matrices	1177:1184	arg1	strength					1161:1168	the mechanical strength	1146:1168	the mechanical strength of the matrices	1146:1184	The combination of psyllium and grewia polysaccharides in the matrices resulted in a significant increase in the mechanical strength of the matrices when compared to matrices containing psyllium alone as the matrix former.					
31972440	6	75	dep	strength	942:949	arg1	95.70 N					965:971	95.70 N	965:971	95.70 N	965:971	The results show that GG and GDS produced tablets with good mechanical strength (108.33 N and 95.70 N, respectively) while psyllium produced softer tablets (7.13 N).					
31972440	6	75	dep	strength	942:949	arg1	108.33 N					952:959	108.33 N	952:959	108.33 N	952:959	The results show that GG and GDS produced tablets with good mechanical strength (108.33 N and 95.70 N, respectively) while psyllium produced softer tablets (7.13 N).					
31972440	2	76	theme	polysaccharides	431:445	arg1	mixtures					415:422	binary mixtures	408:422	binary mixtures of the polysaccharides with psyllium	408:459	Two grades of grewia polysaccharides (GG and GDS) were extracted and binary mixtures of the polysaccharides with psyllium were formulated into tablet matrices containing theophylline as the model drug.					
31972440	9	77	theme	GDS	1452:1454	arg1	matrices					1434:1441	The matrices	1430:1441	The matrices of GG or GDS	1430:1454	The matrices of GG or GDS and the binary mixtures conform to non-Fickian anomalous diffusion with n > 0.45.					
31972440	9	77	theme	GDS	1452:1454	arg1	mixtures					1471:1478	the binary mixtures	1460:1478	the binary mixtures	1460:1478	The matrices of GG or GDS and the binary mixtures conform to non-Fickian anomalous diffusion with n > 0.45.					
33205457	7	0	dep	RESULTS	878:884	arg1	showed					898:903	showed	898:903	showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2	898:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	10	1	theme	flocculation	1922:1933	arg1	stabilities					1935:1945	excellent flocculation stabilities	1912:1945	excellent flocculation stabilities	1912:1945	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	10	2	theme	excellent	1912:1920	arg1	stabilities					1935:1945	excellent flocculation stabilities	1912:1945	excellent flocculation stabilities	1912:1945	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	7	3	theme	neutralization	1130:1143	arg1	degree					1120:1125	a high degree	1113:1125	a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2	1113:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	4	theme	2	1003:1003	arg1	pH					997:998	a pH	995:998	a pH of 2 (ChN1 -F1 -pH 2)	995:1020	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	10	5	theme	-F1	1767:1769	arg1	emulsion					1777:1784	The ChN1 -F1 -pH 2 emulsion	1758:1784	The ChN1 -F1 -pH 2 emulsion	1758:1784	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	12	6	theme	microstructure	2148:2161	arg1	degree					2175:2180	the microstructure and binding degree	2144:2180	the microstructure and binding degree of the complexes	2144:2197	Furthermore, by changing the mass ratio and pH, the microstructure and binding degree of the complexes can be adjusted, thereby promoting their adsorption on the oil-water interface and improving the stability of the Pickering emulsion.					
33205457	11	7	theme	polysaccharide	2030:2043	arg1	particles					2045:2053	the oppositely charged polysaccharide particles	2007:2053	the oppositely charged polysaccharide particles (ChN-F complexes)	2007:2071	CONCLUSION The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes) reduced significantly.					
33205457	11	7	theme	polysaccharide	2030:2043	arg1	complexes					2062:2070	ChN-F complexes	2056:2070	ChN-F complexes	2056:2070	CONCLUSION The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes) reduced significantly.					
33205457	13	8	theme	Chemical	2351:2358	arg1	Industry					2360:2367	Chemical Industry	2351:2367	Chemical Industry	2351:2367	© 2020 Society of Chemical Industry.					
33205457	12	9	theme	binding	2167:2173	arg1	degree					2175:2180	the microstructure and binding degree	2144:2180	the microstructure and binding degree of the complexes	2144:2197	Furthermore, by changing the mass ratio and pH, the microstructure and binding degree of the complexes can be adjusted, thereby promoting their adsorption on the oil-water interface and improving the stability of the Pickering emulsion.					
33205457	10	10	theme	excellent	1796:1804	arg1	stability					1814:1822	excellent storage stability	1796:1822	excellent storage stability	1796:1822	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	12	11	from	adsorption	2240:2249	arg1	interface					2268:2276	the oil-water interface	2254:2276	the oil-water interface	2254:2276	Furthermore, by changing the mass ratio and pH, the microstructure and binding degree of the complexes can be adjusted, thereby promoting their adsorption on the oil-water interface and improving the stability of the Pickering emulsion.					
33205457	7	12	from	Meanwhile	1237:1245	arg1	neutralization					1130:1143	neutralization	1130:1143	neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2	1130:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	2	13	theme	unique	260:265	arg1	properties					276:285	Their unique cationic properties	254:285	Their unique cationic properties	254:285	Their unique cationic properties and antibacterial activity have generated considerable interest among researchers.					
33205457	7	14	theme	sulfate	1043:1049	arg1	content					1051:1057	the lowest sulfate content	1032:1057	the lowest sulfate content (20.1%)	1032:1065	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	14	theme	sulfate	1043:1049	arg1	%					1064:1064	20.1%	1060:1064	20.1%	1060:1064	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	15	from	group	1225:1229	arg1	Meanwhile					1237:1245	F. Meanwhile	1234:1245	F. Meanwhile	1234:1245	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	15	from	group	1225:1229	arg1	ChN					1186:1188	ChN	1186:1188	ChN	1186:1188	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	16	theme	angle	1357:1361	arg1	9.3°					1365:1368	contact angle = 9.3°	1349:1368	contact angle = 9.3°	1349:1368	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	16	theme	angle	1357:1361	arg1	wettability					1336:1346	wettability	1336:1346	wettability (contact angle = 9.3°)	1336:1369	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	12	17	theme	mass	2125:2128	arg1	ratio					2130:2134	the mass ratio	2121:2134	the mass ratio	2121:2134	Furthermore, by changing the mass ratio and pH, the microstructure and binding degree of the complexes can be adjusted, thereby promoting their adsorption on the oil-water interface and improving the stability of the Pickering emulsion.					
33205457	9	18	theme	Pickering	1599:1607	arg1	emulsion					1609:1616	the Pickering emulsion	1595:1616	the Pickering emulsion stabilized by ChN-F	1595:1636	Furthermore, the particle size of the Pickering emulsion stabilized by ChN-F was significantly reduced, and the maximum size did not exceed 10 μm; the physical and storage stability also improved.					
33205457	2	19	theme	antibacterial	291:303	arg1	activity					305:312	antibacterial activity	291:312	antibacterial activity	291:312	Their unique cationic properties and antibacterial activity have generated considerable interest among researchers.					
33205457	6	20	theme	charged	732:738	arg1	fucoidan					740:747	negatively charged fucoidan	721:747	negatively charged fucoidan	721:747	Positively charged ChN and negatively charged fucoidan (F) were therefore compounded to stabilize Pickering emulsions, and the stability of these emulsions was analyzed qualitatively.					
33205457	7	21	theme	F.	1234:1235	arg1	Meanwhile					1237:1245	F. Meanwhile	1234:1245	F. Meanwhile	1234:1245	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	0	22	theme	emulsion	23:30	arg1	stabilization					32:44	Oil-in-water Pickering emulsion stabilization	0:44	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles	0:93	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles: chitin nanocrystals/fucoidan complexes.					
33205457	1	23	theme	insoluble	177:185	arg1	nanocrystals					154:165	BACKGROUND Chitin nanocrystals	136:165	BACKGROUND Chitin nanocrystals (ChN)	136:171	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	1	23	theme	insoluble	177:185	arg1	particles					187:195	insoluble particles	177:195	insoluble particles that can be used as stabilizers for Pickering emulsions	177:251	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	1	23	theme	insoluble	177:185	arg1	stabilizers					217:227	stabilizers	217:227	stabilizers for Pickering emulsions	217:251	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	4	24	theme	further	546:552	arg1	improvement					554:564	further improvement	546:564	further improvement	546:564	Furthermore, the droplets of the emulsions stabilized by ChN are as large as 10-100 μm, and their physical stability requires further improvement.					
33205457	6	25	theme	charged	705:711	arg1	ChN					713:715	Positively charged ChN	694:715	Positively charged ChN	694:715	Positively charged ChN and negatively charged fucoidan (F) were therefore compounded to stabilize Pickering emulsions, and the stability of these emulsions was analyzed qualitatively.					
33205457	6	25	theme	charged	705:711	arg1	F					750:750	F	750:750	F	750:750	Positively charged ChN and negatively charged fucoidan (F) were therefore compounded to stabilize Pickering emulsions, and the stability of these emulsions was analyzed qualitatively.					
33205457	0	26	theme	chitin	96:101	arg1	complexes					125:133	chitin nanocrystals/fucoidan complexes	96:133	chitin nanocrystals/fucoidan complexes	96:133	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles: chitin nanocrystals/fucoidan complexes.					
33205457	7	27	theme	-F1	1011:1013	arg1	2					1003:1003	2	1003:1003	2	1003:1003	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	27	theme	-F1	1011:1013	arg1	-pH 2					1015:1019	ChN1 -F1 -pH 2	1006:1019	ChN1 -F1 -pH 2	1006:1019	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	8	28	dep	transform	1380:1388	arg1	infrared					1390:1397	infrared	1390:1397	transform infrared spectroscopy results	1380:1418	Fourier transform infrared spectroscopy results confirmed that ChN and F were effectively combined through electrostatic interaction or neutralization to produce a polyelectrolyte complex.					
33205457	1	29	used	used	209:212	arg2	nanocrystals					154:165	BACKGROUND Chitin nanocrystals	136:165	BACKGROUND Chitin nanocrystals (ChN)	136:171	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	1	29	used	used	209:212	arg2	particles					187:195	insoluble particles	177:195	insoluble particles that can be used as stabilizers for Pickering emulsions	177:251	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	1	29	used	used	209:212	arg2	stabilizers					217:227	stabilizers	217:227	stabilizers for Pickering emulsions	217:251	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	1	30	theme	Chitin	147:152	arg1	ChN					168:170	ChN	168:170	ChN	168:170	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	1	30	theme	Chitin	147:152	arg1	nanocrystals					154:165	BACKGROUND Chitin nanocrystals	136:165	BACKGROUND Chitin nanocrystals (ChN)	136:171	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	1	30	theme	Chitin	147:152	arg1	particles					187:195	insoluble particles	177:195	insoluble particles that can be used as stabilizers for Pickering emulsions	177:251	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	7	31	theme	charged	1209:1215	arg1	group					1225:1229	the negatively charged sulfate group	1194:1229	the negatively charged sulfate group in F. Meanwhile	1194:1245	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	31	theme	charged	1209:1215	arg1	-pH 2					1257:1261	ChN1 -F1 -pH 2	1248:1261	ChN1 -F1 -pH 2	1248:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	2	32	theme	considerable	329:340	arg1	interest					342:349	considerable interest	329:349	considerable interest among researchers	329:367	Their unique cationic properties and antibacterial activity have generated considerable interest among researchers.					
33205457	7	33	from	group	1177:1181	arg1	Meanwhile					1237:1245	F. Meanwhile	1234:1245	F. Meanwhile	1234:1245	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	33	from	group	1177:1181	arg1	ChN					1186:1188	ChN	1186:1188	ChN	1186:1188	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	0	34	dep	complexes	125:133	arg1	stabilization					32:44	Oil-in-water Pickering emulsion stabilization	0:44	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles	0:93	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles: chitin nanocrystals/fucoidan complexes.					
33205457	7	35	from	ChN	1186:1188	arg1	neutralization					1130:1143	neutralization	1130:1143	neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2	1130:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	0	36	theme	Oil-in-water	0:11	arg1	stabilization					32:44	Oil-in-water Pickering emulsion stabilization	0:44	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles	0:93	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles: chitin nanocrystals/fucoidan complexes.					
33205457	5	37	theme	particles	643:651	arg1	reaction					612:619	the spontaneous reaction	596:619	the spontaneous reaction of oppositely charged particles	596:651	Some studies have shown that the spontaneous reaction of oppositely charged particles can effectively stabilize the emulsions.					
33205457	7	38	theme	group	1177:1181	arg1	neutralization					1130:1143	neutralization	1130:1143	neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2	1130:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	11	39	theme	charged	2022:2028	arg1	particles					2045:2053	the oppositely charged polysaccharide particles	2007:2053	the oppositely charged polysaccharide particles (ChN-F complexes)	2007:2071	CONCLUSION The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes) reduced significantly.					
33205457	11	39	theme	charged	2022:2028	arg1	complexes					2062:2070	ChN-F complexes	2056:2070	ChN-F complexes	2056:2070	CONCLUSION The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes) reduced significantly.					
33205457	9	40	theme	emulsion	1609:1616	arg1	size					1587:1590	the particle size	1574:1590	the particle size of the Pickering emulsion stabilized by ChN-F	1574:1636	Furthermore, the particle size of the Pickering emulsion stabilized by ChN-F was significantly reduced, and the maximum size did not exceed 10 μm; the physical and storage stability also improved.					
33205457	10	41	theme	-F1	1873:1875	arg1	-pH 5					1877:1881	ChN1 -F1 -pH 5	1868:1881	ChN1 -F1 -pH 5	1868:1881	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	7	42	theme	charged	1163:1169	arg1	group					1177:1181	the positively charged amino group	1148:1181	the positively charged amino group in ChN	1148:1188	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	0	43	theme	charged	62:68	arg1	particles					85:93	oppositely charged polysaccharide particles	51:93	oppositely charged polysaccharide particles	51:93	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles: chitin nanocrystals/fucoidan complexes.					
33205457	7	44	theme	dense	1275:1279	arg1	structure					1289:1297	a dense network structure	1273:1297	a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°)	1273:1369	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	45	from	neutralization	1130:1143	arg1	Meanwhile					1237:1245	F. Meanwhile	1234:1245	F. Meanwhile	1234:1245	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	45	from	neutralization	1130:1143	arg1	ChN					1186:1188	ChN	1186:1188	ChN	1186:1188	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	10	46	theme	-F1	1892:1894	arg1	-pH 6					1896:1900	ChN1 -F1 -pH 6	1887:1900	ChN1 -F1 -pH 6	1887:1900	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	11	47	dep	CONCLUSION	1948:1957	arg1	reduced					2073:2079	reduced	2073:2079	reduced significantly	2073:2093	CONCLUSION The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes) reduced significantly.					
33205457	7	48	contain	possessed	1022:1030	arg2	-3 mV					1094:1098	-3 mV	1094:1098	-3 mV	1094:1098	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	48	contain	possessed	1022:1030	arg2	%					1064:1064	20.1%	1060:1064	20.1%	1060:1064	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	48	contain	possessed	1022:1030	arg2	content					1051:1057	the lowest sulfate content	1032:1057	the lowest sulfate content (20.1%)	1032:1065	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	48	contain	possessed	1022:1030	arg2	potential					1083:1091	almost zero potential	1071:1091	almost zero potential (-3 mV)	1071:1099	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	48	contain	possessed	1022:1030	arg1	particles					924:932	the composite particles	910:932	the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2)	910:1020	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	12	49	theme	complexes	2189:2197	arg1	degree					2175:2180	the microstructure and binding degree	2144:2180	the microstructure and binding degree of the complexes	2144:2197	Furthermore, by changing the mass ratio and pH, the microstructure and binding degree of the complexes can be adjusted, thereby promoting their adsorption on the oil-water interface and improving the stability of the Pickering emulsion.					
33205457	7	50	theme	1:1	984:986	arg1	ratio					975:979	a mass ratio	968:979	a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2)	968:1020	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	51	theme	-F1	1253:1255	arg1	group					1225:1229	the negatively charged sulfate group	1194:1229	the negatively charged sulfate group in F. Meanwhile	1194:1245	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	51	theme	-F1	1253:1255	arg1	-pH 2					1257:1261	ChN1 -F1 -pH 2	1248:1261	ChN1 -F1 -pH 2	1248:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	52	theme	sulfate	1217:1223	arg1	group					1225:1229	the negatively charged sulfate group	1194:1229	the negatively charged sulfate group in F. Meanwhile	1194:1245	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	52	theme	sulfate	1217:1223	arg1	-pH 2					1257:1261	ChN1 -F1 -pH 2	1248:1261	ChN1 -F1 -pH 2	1248:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	6	53	theme	Pickering	792:800	arg1	emulsions					802:810	Pickering emulsions	792:810	Pickering emulsions	792:810	Positively charged ChN and negatively charged fucoidan (F) were therefore compounded to stabilize Pickering emulsions, and the stability of these emulsions was analyzed qualitatively.					
33205457	9	54	theme	maximum	1673:1679	arg1	size					1681:1684	the maximum size	1669:1684	the maximum size	1669:1684	Furthermore, the particle size of the Pickering emulsion stabilized by ChN-F was significantly reduced, and the maximum size did not exceed 10 μm; the physical and storage stability also improved.					
33205457	4	55	theme	emulsions	453:461	arg1	droplets					437:444	the droplets	433:444	the droplets of the emulsions stabilized by ChN	433:479	Furthermore, the droplets of the emulsions stabilized by ChN are as large as 10-100 μm, and their physical stability requires further improvement.					
33205457	4	55	theme	emulsions	453:461	arg1	large					488:492	large	488:492	large	488:492	Furthermore, the droplets of the emulsions stabilized by ChN are as large as 10-100 μm, and their physical stability requires further improvement.					
33205457	7	56	theme	high	1115:1118	arg1	degree					1120:1125	a high degree	1113:1125	a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2	1113:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	10	57	theme	-pH 2	1771:1775	arg1	emulsion					1777:1784	The ChN1 -F1 -pH 2 emulsion	1758:1784	The ChN1 -F1 -pH 2 emulsion	1758:1784	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	10	58	theme	ChN1	1762:1765	arg1	emulsion					1777:1784	The ChN1 -F1 -pH 2 emulsion	1758:1784	The ChN1 -F1 -pH 2 emulsion	1758:1784	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	8	59	dep	Fourier	1372:1378	arg1	transform					1380:1388	transform	1380:1388	transform infrared spectroscopy results	1380:1418	Fourier transform infrared spectroscopy results confirmed that ChN and F were effectively combined through electrostatic interaction or neutralization to produce a polyelectrolyte complex.					
33205457	7	60	theme	group	1225:1229	arg1	neutralization					1130:1143	neutralization	1130:1143	neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2	1130:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	10	61	theme	storage	1806:1812	arg1	stability					1814:1822	excellent storage stability	1796:1822	excellent storage stability	1796:1822	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	6	62	theme	emulsions	840:848	arg1	stability					821:829	the stability	817:829	the stability of these emulsions	817:848	Positively charged ChN and negatively charged fucoidan (F) were therefore compounded to stabilize Pickering emulsions, and the stability of these emulsions was analyzed qualitatively.					
33205457	11	63	theme	ChN-F	2056:2060	arg1	particles					2045:2053	the oppositely charged polysaccharide particles	2007:2053	the oppositely charged polysaccharide particles (ChN-F complexes)	2007:2071	CONCLUSION The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes) reduced significantly.					
33205457	11	63	theme	ChN-F	2056:2060	arg1	complexes					2062:2070	ChN-F complexes	2056:2070	ChN-F complexes	2056:2070	CONCLUSION The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes) reduced significantly.					
33205457	7	64	theme	composite	914:922	arg1	particles					924:932	the composite particles	910:932	the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2)	910:1020	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	2	65	theme	cationic	267:274	arg1	properties					276:285	Their unique cationic properties	254:285	Their unique cationic properties	254:285	Their unique cationic properties and antibacterial activity have generated considerable interest among researchers.					
33205457	1	66	theme	Pickering	233:241	arg1	emulsions					243:251	Pickering emulsions	233:251	Pickering emulsions	233:251	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	5	67	theme	spontaneous	600:610	arg1	reaction					612:619	the spontaneous reaction	596:619	the spontaneous reaction of oppositely charged particles	596:651	Some studies have shown that the spontaneous reaction of oppositely charged particles can effectively stabilize the emulsions.					
33205457	12	68	theme	Pickering	2313:2321	arg1	emulsion					2323:2330	the Pickering emulsion	2309:2330	the Pickering emulsion	2309:2330	Furthermore, by changing the mass ratio and pH, the microstructure and binding degree of the complexes can be adjusted, thereby promoting their adsorption on the oil-water interface and improving the stability of the Pickering emulsion.					
33205457	9	69	dep	physical	1712:1719	arg1	stability					1733:1741	stability	1733:1741	stability	1733:1741	Furthermore, the particle size of the Pickering emulsion stabilized by ChN-F was significantly reduced, and the maximum size did not exceed 10 μm; the physical and storage stability also improved.					
33205457	8	70	theme	electrostatic	1479:1491	arg1	interaction					1493:1503	electrostatic interaction	1479:1503	electrostatic interaction	1479:1503	Fourier transform infrared spectroscopy results confirmed that ChN and F were effectively combined through electrostatic interaction or neutralization to produce a polyelectrolyte complex.					
33205457	7	71	from	pH	997:998	arg1	ratio					975:979	a mass ratio	968:979	a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2)	968:1020	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	71	from	pH	997:998	arg1	polysaccharides					949:963	two polysaccharides	945:963	two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2)	945:1020	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	72	theme	=	1363:1363	arg1	9.3°					1365:1368	contact angle = 9.3°	1349:1368	contact angle = 9.3°	1349:1368	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	72	theme	=	1363:1363	arg1	wettability					1336:1346	wettability	1336:1346	wettability (contact angle = 9.3°)	1336:1369	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	73	theme	lowest	1036:1041	arg1	content					1051:1057	the lowest sulfate content	1032:1057	the lowest sulfate content (20.1%)	1032:1065	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	73	theme	lowest	1036:1041	arg1	%					1064:1064	20.1%	1060:1064	20.1%	1060:1064	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	74	theme	contact	1349:1355	arg1	9.3°					1365:1368	contact angle = 9.3°	1349:1368	contact angle = 9.3°	1349:1368	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	74	theme	contact	1349:1355	arg1	wettability					1336:1346	wettability	1336:1346	wettability (contact angle = 9.3°)	1336:1369	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	0	75	theme	nanocrystals/fucoidan	103:123	arg1	complexes					125:133	chitin nanocrystals/fucoidan complexes	96:133	chitin nanocrystals/fucoidan complexes	96:133	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles: chitin nanocrystals/fucoidan complexes.					
33205457	12	76	theme	emulsion	2323:2330	arg1	stability					2296:2304	the stability	2292:2304	the stability of the Pickering emulsion	2292:2330	Furthermore, by changing the mass ratio and pH, the microstructure and binding degree of the complexes can be adjusted, thereby promoting their adsorption on the oil-water interface and improving the stability of the Pickering emulsion.					
33205457	4	77	theme	physical	518:525	arg1	stability					527:535	their physical stability	512:535	their physical stability	512:535	Furthermore, the droplets of the emulsions stabilized by ChN are as large as 10-100 μm, and their physical stability requires further improvement.					
33205457	7	78	theme	ChN1	1006:1009	arg1	2					1003:1003	2	1003:1003	2	1003:1003	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	78	theme	ChN1	1006:1009	arg1	-pH 2					1015:1019	ChN1 -F1 -pH 2	1006:1019	ChN1 -F1 -pH 2	1006:1019	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	9	79	theme	particle	1578:1585	arg1	size					1587:1590	the particle size	1574:1590	the particle size of the Pickering emulsion stabilized by ChN-F	1574:1636	Furthermore, the particle size of the Pickering emulsion stabilized by ChN-F was significantly reduced, and the maximum size did not exceed 10 μm; the physical and storage stability also improved.					
33205457	12	80	theme	oil-water	2258:2266	arg1	interface					2268:2276	the oil-water interface	2254:2276	the oil-water interface	2254:2276	Furthermore, by changing the mass ratio and pH, the microstructure and binding degree of the complexes can be adjusted, thereby promoting their adsorption on the oil-water interface and improving the stability of the Pickering emulsion.					
33205457	0	81	theme	Pickering	13:21	arg1	stabilization					32:44	Oil-in-water Pickering emulsion stabilization	0:44	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles	0:93	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles: chitin nanocrystals/fucoidan complexes.					
33205457	5	82	theme	charged	635:641	arg1	particles					643:651	oppositely charged particles	624:651	oppositely charged particles	624:651	Some studies have shown that the spontaneous reaction of oppositely charged particles can effectively stabilize the emulsions.					
33205457	8	83	theme	polyelectrolyte	1536:1550	arg1	complex					1552:1558	a polyelectrolyte complex	1534:1558	a polyelectrolyte complex	1534:1558	Fourier transform infrared spectroscopy results confirmed that ChN and F were effectively combined through electrostatic interaction or neutralization to produce a polyelectrolyte complex.					
33205457	7	84	theme	amino	1171:1175	arg1	group					1177:1181	the positively charged amino group	1148:1181	the positively charged amino group in ChN	1148:1188	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	1	85	theme	BACKGROUND	136:145	arg1	ChN					168:170	ChN	168:170	ChN	168:170	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	1	85	theme	BACKGROUND	136:145	arg1	nanocrystals					154:165	BACKGROUND Chitin nanocrystals	136:165	BACKGROUND Chitin nanocrystals (ChN)	136:171	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	1	85	theme	BACKGROUND	136:145	arg1	particles					187:195	insoluble particles	177:195	insoluble particles that can be used as stabilizers for Pickering emulsions	177:251	BACKGROUND Chitin nanocrystals (ChN) are insoluble particles that can be used as stabilizers for Pickering emulsions.					
33205457	11	86	theme	emulsion	1975:1982	arg1	droplets					1984:1991	the emulsion droplets	1971:1991	the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes)	1971:2071	CONCLUSION The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes) reduced significantly.					
33205457	7	87	theme	network	1281:1287	arg1	structure					1289:1297	a dense network structure	1273:1297	a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°)	1273:1369	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	10	88	theme	ChN1	1868:1871	arg1	-pH 5					1877:1881	ChN1 -F1 -pH 5	1868:1881	ChN1 -F1 -pH 5	1868:1881	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	0	89	with	stabilization	32:44	arg1	particles					85:93	oppositely charged polysaccharide particles	51:93	oppositely charged polysaccharide particles	51:93	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles: chitin nanocrystals/fucoidan complexes.					
33205457	0	90	theme	polysaccharide	70:83	arg1	particles					85:93	oppositely charged polysaccharide particles	51:93	oppositely charged polysaccharide particles	51:93	Oil-in-water Pickering emulsion stabilization with oppositely charged polysaccharide particles: chitin nanocrystals/fucoidan complexes.					
33205457	8	91	theme	spectroscopy	1399:1410	arg1	results					1412:1418	spectroscopy results	1399:1418	spectroscopy results	1399:1418	Fourier transform infrared spectroscopy results confirmed that ChN and F were effectively combined through electrostatic interaction or neutralization to produce a polyelectrolyte complex.					
33205457	11	92	theme	droplets	1984:1991	arg1	size					1963:1966	The size	1959:1966	The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes)	1959:2071	CONCLUSION The size of the emulsion droplets stabilized by the oppositely charged polysaccharide particles (ChN-F complexes) reduced significantly.					
33205457	10	93	theme	ChN1	1887:1890	arg1	-pH 6					1896:1900	ChN1 -F1 -pH 6	1887:1900	ChN1 -F1 -pH 6	1887:1900	The ChN1 -F1 -pH 2 emulsion presented excellent storage stability; in particular, the emulsions stabilized by ChN1 -F1 -pH 5 and ChN1 -F1 -pH 6 exhibited excellent flocculation stabilities.					
33205457	7	94	theme	ChN1	1248:1251	arg1	group					1225:1229	the negatively charged sulfate group	1194:1229	the negatively charged sulfate group in F. Meanwhile	1194:1245	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	94	theme	ChN1	1248:1251	arg1	-pH 2					1257:1261	ChN1 -F1 -pH 2	1248:1261	ChN1 -F1 -pH 2	1248:1261	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	95	from	polysaccharides	949:963	arg1	ratio					975:979	a mass ratio	968:979	a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2)	968:1020	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	95	from	polysaccharides	949:963	arg1	pH					997:998	a pH	995:998	a pH of 2 (ChN1 -F1 -pH 2)	995:1020	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
33205457	7	96	theme	mass	970:973	arg1	ratio					975:979	a mass ratio	968:979	a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2)	968:1020	RESULTS The results showed that the composite particles comprising two polysaccharides in a mass ratio of 1:1 and at a pH of 2 (ChN1 -F1 -pH 2) possessed the lowest sulfate content (20.1%) and almost zero potential (-3 mV), indicating a high degree of neutralization of the positively charged amino group in ChN and the negatively charged sulfate group in F. Meanwhile, ChN1 -F1 -pH 2 displayed a dense network structure that improved the dispersibility and wettability (contact angle = 9.3°).					
34780896	4	0	theme	scanning	573:580	arg1	Fourier					603:609	Fourier	603:609	Fourier transform infrared spectroscopy and various rheological methods	603:673	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	4	0	theme	scanning	573:580	arg1	microscopy					591:600	The scanning electron microscopy	569:600	The scanning electron microscopy	569:600	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	1	1	theme	physicochemical	147:161	arg1	properties					187:196	unique physicochemical and variable mechanical properties	140:196	unique physicochemical and variable mechanical properties	140:196	Hydrogel, as a three-dimensional material with high water content, has unique physicochemical and variable mechanical properties.					
34780896	5	2	theme	Arrhenius	877:885	arg1	models					887:892	Arrhenius models	877:892	Arrhenius models	877:892	The key rheological parameters were compared using the Power Law, Herschel-Bulkley and Arrhenius models.					
34780896	5	2	theme	Arrhenius	877:885	arg1	Law					851:853	the Power Law	841:853	the Power Law	841:853	The key rheological parameters were compared using the Power Law, Herschel-Bulkley and Arrhenius models.					
34780896	4	3	dep	transform	611:619	arg1	infrared					621:628	infrared	621:628	transform infrared spectroscopy and various rheological methods	611:673	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	4	4	dep	morphology	708:717	arg1	the					704:706	the	704:706	the	704:706	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	3	5	from	polysaccharides	470:484	arg1	engineering					416:426	the engineering	412:426	the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan	412:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	3	5	from	polysaccharides	470:484	arg1	hydrogels					447:455	novel composite hydrogels	431:455	novel composite hydrogels from natural polysaccharides salecan and curdlan	431:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	3	6	from	engineering	416:426	arg1	curdlan					498:504	curdlan	498:504	curdlan	498:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	3	6	from	engineering	416:426	arg1	salecan					486:492	salecan	486:492	salecan	486:492	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	3	6	from	engineering	416:426	arg1	polysaccharides					470:484	natural polysaccharides salecan and curdlan	462:504	natural polysaccharides salecan and curdlan	462:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	6	7	from	application	1129:1139	arg1	fields					1154:1159	different fields	1144:1159	different fields	1144:1159	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	6	8	theme	first	907:911	arg1	This					895:898	This	895:898	This	895:898	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	6	8	theme	first	907:911	arg1	study					913:917	the first study	903:917	the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields	903:1159	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	0	9	theme	Rheological	0:10	arg1	investigation					12:24	Rheological investigation	0:24	Rheological investigation of a versatile salecan/curdlan gel matrix	0:66	Rheological investigation of a versatile salecan/curdlan gel matrix.					
34780896	5	10	theme	Power	845:849	arg1	Herschel-Bulkley					856:871	Herschel-Bulkley	856:871	Herschel-Bulkley	856:871	The key rheological parameters were compared using the Power Law, Herschel-Bulkley and Arrhenius models.					
34780896	5	10	theme	Power	845:849	arg1	models					887:892	Arrhenius models	877:892	Arrhenius models	877:892	The key rheological parameters were compared using the Power Law, Herschel-Bulkley and Arrhenius models.					
34780896	5	10	theme	Power	845:849	arg1	Law					851:853	the Power Law	841:853	the Power Law	841:853	The key rheological parameters were compared using the Power Law, Herschel-Bulkley and Arrhenius models.					
34780896	6	11	theme	ideal	991:995	arg1	elasticity					997:1006	ideal elasticity	991:1006	ideal elasticity	991:1006	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	1	12	theme	variable	167:174	arg1	properties					187:196	unique physicochemical and variable mechanical properties	140:196	unique physicochemical and variable mechanical properties	140:196	Hydrogel, as a three-dimensional material with high water content, has unique physicochemical and variable mechanical properties.					
34780896	3	13	theme	natural	462:468	arg1	curdlan					498:504	curdlan	498:504	curdlan	498:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	3	13	theme	natural	462:468	arg1	salecan					486:492	salecan	486:492	salecan	486:492	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	3	13	theme	natural	462:468	arg1	polysaccharides					470:484	natural polysaccharides salecan and curdlan	462:504	natural polysaccharides salecan and curdlan	462:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	2	14	theme	viscoelastic	298:309	arg1	materials					311:319	these viscoelastic materials	292:319	these viscoelastic materials	292:319	Natural polysaccharide-based composite hydrogels are very popular within medical industry as these viscoelastic materials are non-toxic, biodegradable, bioabsorbable, and biocompatible.					
34780896	1	15	theme	mechanical	176:185	arg1	properties					187:196	unique physicochemical and variable mechanical properties	140:196	unique physicochemical and variable mechanical properties	140:196	Hydrogel, as a three-dimensional material with high water content, has unique physicochemical and variable mechanical properties.					
34780896	1	16	with	material	102:109	arg1	content					127:133	high water content	116:133	high water content	116:133	Hydrogel, as a three-dimensional material with high water content, has unique physicochemical and variable mechanical properties.					
34780896	1	17	contain	has	136:138	arg1	Hydrogel					69:76	Hydrogel	69:76	Hydrogel	69:76	Hydrogel, as a three-dimensional material with high water content, has unique physicochemical and variable mechanical properties.					
34780896	1	17	contain	has	136:138	arg2	properties					187:196	unique physicochemical and variable mechanical properties	140:196	unique physicochemical and variable mechanical properties	140:196	Hydrogel, as a three-dimensional material with high water content, has unique physicochemical and variable mechanical properties.					
34780896	4	18	theme	samples	768:774	arg1	morphology					708:717	morphology	708:717	morphology	708:717	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	4	18	theme	samples	768:774	arg1	interaction					730:740	molecular interaction	720:740	molecular interaction	720:740	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	4	18	theme	samples	768:774	arg1	behavior					752:759	flow behavior	747:759	flow behavior	747:759	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	3	19	theme	composite	437:445	arg1	hydrogels					447:455	novel composite hydrogels	431:455	novel composite hydrogels from natural polysaccharides salecan and curdlan	431:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	4	20	theme	various	647:653	arg1	methods					667:673	various rheological methods	647:673	various rheological methods	647:673	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	3	21	dep	polysaccharides	470:484	arg1	curdlan					498:504	curdlan	498:504	curdlan	498:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	3	21	dep	polysaccharides	470:484	arg1	salecan					486:492	salecan	486:492	salecan	486:492	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	3	21	dep	polysaccharides	470:484	arg1	polysaccharides					470:484	natural polysaccharides salecan and curdlan	462:504	natural polysaccharides salecan and curdlan	462:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	4	22	dep	Fourier	603:609	arg1	transform					611:619	transform	611:619	transform infrared spectroscopy and various rheological methods	611:673	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	1	23	theme	three-dimensional	84:100	arg1	material					102:109	a three-dimensional material	82:109	a three-dimensional material with high water content	82:133	Hydrogel, as a three-dimensional material with high water content, has unique physicochemical and variable mechanical properties.					
34780896	6	24	theme	rheological	1079:1089	arg1	properties					1091:1100	other rheological properties	1073:1100	other rheological properties that will pave the way for application in different fields	1073:1159	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	5	25	theme	rheological	798:808	arg1	parameters					810:819	The key rheological parameters	790:819	The key rheological parameters	790:819	The key rheological parameters were compared using the Power Law, Herschel-Bulkley and Arrhenius models.					
34780896	6	26	theme	other	1073:1077	arg1	properties					1091:1100	other rheological properties	1073:1100	other rheological properties that will pave the way for application in different fields	1073:1159	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	0	27	theme	salecan/curdlan	41:55	arg1	matrix					61:66	a versatile salecan/curdlan gel matrix	29:66	a versatile salecan/curdlan gel matrix	29:66	Rheological investigation of a versatile salecan/curdlan gel matrix.					
34780896	4	28	theme	rheological	655:665	arg1	methods					667:673	various rheological methods	647:673	various rheological methods	647:673	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	4	29	theme	flow	747:750	arg1	behavior					752:759	flow behavior	747:759	flow behavior	747:759	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	0	30	theme	versatile	31:39	arg1	matrix					61:66	a versatile salecan/curdlan gel matrix	29:66	a versatile salecan/curdlan gel matrix	29:66	Rheological investigation of a versatile salecan/curdlan gel matrix.					
34780896	3	31	theme	structural	518:527	arg1	modification					529:540	structural modification	518:540	structural modification	518:540	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	0	32	theme	matrix	61:66	arg1	investigation					12:24	Rheological investigation	0:24	Rheological investigation of a versatile salecan/curdlan gel matrix	0:66	Rheological investigation of a versatile salecan/curdlan gel matrix.					
34780896	6	33	theme	different	1144:1152	arg1	fields					1154:1159	different fields	1144:1159	different fields	1144:1159	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	1	34	theme	high	116:119	arg1	content					127:133	high water content	116:133	high water content	116:133	Hydrogel, as a three-dimensional material with high water content, has unique physicochemical and variable mechanical properties.					
34780896	0	35	theme	gel	57:59	arg1	matrix					61:66	a versatile salecan/curdlan gel matrix	29:66	a versatile salecan/curdlan gel matrix	29:66	Rheological investigation of a versatile salecan/curdlan gel matrix.					
34780896	6	36	theme	enhanced	1009:1016	arg1	thermostability					1018:1032	enhanced thermostability	1009:1032	enhanced thermostability	1009:1032	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	6	37	theme	composite	937:945	arg1	hydrogel					947:954	a novel composite hydrogel	929:954	a novel composite hydrogel made from Salecan and Curdlan	929:984	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	1	38	theme	water	121:125	arg1	content					127:133	high water content	116:133	high water content	116:133	Hydrogel, as a three-dimensional material with high water content, has unique physicochemical and variable mechanical properties.					
34780896	6	39	theme	good	1035:1038	arg1	injectability					1040:1052	good injectability	1035:1052	good injectability	1035:1052	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	2	40	theme	composite	228:236	arg1	hydrogels					238:246	Natural polysaccharide-based composite hydrogels	199:246	Natural polysaccharide-based composite hydrogels	199:246	Natural polysaccharide-based composite hydrogels are very popular within medical industry as these viscoelastic materials are non-toxic, biodegradable, bioabsorbable, and biocompatible.					
34780896	3	41	theme	chemical	546:553	arg1	crosslinking					555:566	chemical crosslinking	546:566	chemical crosslinking	546:566	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	5	42	theme	key	794:796	arg1	parameters					810:819	The key rheological parameters	790:819	The key rheological parameters	790:819	The key rheological parameters were compared using the Power Law, Herschel-Bulkley and Arrhenius models.					
34780896	2	43	theme	polysaccharide-based	207:226	arg1	hydrogels					238:246	Natural polysaccharide-based composite hydrogels	199:246	Natural polysaccharide-based composite hydrogels	199:246	Natural polysaccharide-based composite hydrogels are very popular within medical industry as these viscoelastic materials are non-toxic, biodegradable, bioabsorbable, and biocompatible.					
34780896	1	44	theme	unique	140:145	arg1	properties					187:196	unique physicochemical and variable mechanical properties	140:196	unique physicochemical and variable mechanical properties	140:196	Hydrogel, as a three-dimensional material with high water content, has unique physicochemical and variable mechanical properties.					
34780896	2	45	theme	Natural	199:205	arg1	hydrogels					238:246	Natural polysaccharide-based composite hydrogels	199:246	Natural polysaccharide-based composite hydrogels	199:246	Natural polysaccharide-based composite hydrogels are very popular within medical industry as these viscoelastic materials are non-toxic, biodegradable, bioabsorbable, and biocompatible.					
34780896	3	46	dep	modification	529:540	arg1	any					514:516	any	514:516	any	514:516	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	6	47	theme	novel	931:935	arg1	hydrogel					947:954	a novel composite hydrogel	929:954	a novel composite hydrogel made from Salecan and Curdlan	929:984	This is the first study reporting a novel composite hydrogel made from Salecan and Curdlan with ideal elasticity, enhanced thermostability, good injectability, self-recovery and other rheological properties that will pave the way for application in different fields.					
34780896	4	48	theme	molecular	720:728	arg1	interaction					730:740	molecular interaction	720:740	molecular interaction	720:740	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	3	49	theme	hydrogels	447:455	arg1	engineering					416:426	the engineering	412:426	the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan	412:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
34780896	4	50	theme	electron	582:589	arg1	Fourier					603:609	Fourier	603:609	Fourier transform infrared spectroscopy and various rheological methods	603:673	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	4	50	theme	electron	582:589	arg1	microscopy					591:600	The scanning electron microscopy	569:600	The scanning electron microscopy	569:600	The scanning electron microscopy, Fourier transform infrared spectroscopy and various rheological methods were employed to investigate the morphology, molecular interaction, and flow behavior of the samples respectively.					
34780896	2	51	theme	medical	272:278	arg1	industry					280:287	medical industry	272:287	medical industry	272:287	Natural polysaccharide-based composite hydrogels are very popular within medical industry as these viscoelastic materials are non-toxic, biodegradable, bioabsorbable, and biocompatible.					
34780896	3	52	theme	novel	431:435	arg1	hydrogels					447:455	novel composite hydrogels	431:455	novel composite hydrogels from natural polysaccharides salecan and curdlan	431:504	This research investigates the engineering of novel composite hydrogels from natural polysaccharides salecan and curdlan without any structural modification and chemical crosslinking.					
33389874	0	0	theme	TM02®	86:90	arg1	Properties					21:30	The Immunomodulating Properties	0:30	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom	0:63	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	0	0	theme	TM02®	86:90	arg1	Agaricomycetes					102:115	Agaricomycetes	102:115	Agaricomycetes	102:115	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	0	0	theme	TM02®	86:90	arg1	Cultivar					92:99	Lignosus rhinocerus TM02® Cultivar	66:99	Lignosus rhinocerus TM02® Cultivar (Agaricomycetes)	66:116	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	4	1	contain	possesses	758:766	arg2	backbone					790:797	1,6-Glcp backbone	781:797	1,6-Glcp backbone	781:797	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	1	contain	possesses	758:766	arg1	fraction					737:744	rhinoprolycan fraction	723:744	rhinoprolycan fraction (HMW & MMW)	723:756	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	1	contain	possesses	758:766	arg1	MMW					753:755	HMW & MMW	747:755	HMW & MMW	747:755	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	1	contain	possesses	758:766	arg2	1,4-Glcp					768:775	1,4-Glcp	768:775	1,4-Glcp	768:775	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	1	contain	possesses	758:766	arg2	chain					812:816	branched chain	803:816	branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp)	803:867	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	1	2	theme	Natural	163:169	arg1	compounds					171:179	Natural compounds	163:179	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes	163:266	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes have the capabilities to modulate the immune system.					
33389874	6	3	dep	in	1166:1167	arg1	vivo					1169:1172	vivo	1169:1172	vivo	1169:1172	Further in vivo studies are needed to fully understand the immunomodulatory effects of TM02®.					
33389874	5	4	theme	3.495	1058:1062	arg1	0.416					1066:1070	3.495 ± 0.416	1058:1070	MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088	1051:1093	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	5	theme	possible	1100:1107	arg1	involvement					1109:1119	possible involvement	1100:1119	possible involvement of NF-κB and MAPK signaling pathway	1100:1155	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	6	theme	±	1064:1064	arg1	0.416					1066:1070	3.495 ± 0.416	1058:1070	MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088	1051:1093	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	7	from	HMW	941:943	arg1	involvement					1109:1119	possible involvement	1100:1119	possible involvement of NF-κB and MAPK signaling pathway	1100:1155	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	7	from	HMW	941:943	arg1	upregulation					895:906	upregulation	895:906	upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088)	895:1094	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	3	8	theme	cultivar	606:613	arg1	RAW					623:625	L. rhinocerus (TM02®) cultivar treated RAW 264.7	584:631	L. rhinocerus (TM02®) cultivar treated RAW 264.7	584:631	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	5	9	theme	3.358	975:979	arg1	0.200					983:987	3.358 ± 0.200	975:987	IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200	946:987	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	3	10	theme	treated	615:621	arg1	RAW					623:625	L. rhinocerus (TM02®) cultivar treated RAW 264.7	584:631	L. rhinocerus (TM02®) cultivar treated RAW 264.7	584:631	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	5	11	from	MMW	991:993	arg1	involvement					1109:1119	possible involvement	1100:1119	possible involvement of NF-κB and MAPK signaling pathway	1100:1155	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	11	from	MMW	991:993	arg1	upregulation					895:906	upregulation	895:906	upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088)	895:1094	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	1	12	theme	immune	306:311	arg1	system					313:318	the immune system	302:318	the immune system	302:318	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes have the capabilities to modulate the immune system.					
33389874	6	13	theme	in	1166:1167	arg1	studies					1174:1180	Further in vivo studies	1158:1180	Further in vivo studies	1158:1180	Further in vivo studies are needed to fully understand the immunomodulatory effects of TM02®.					
33389874	5	14	theme	±	981:981	arg1	0.200					983:987	3.358 ± 0.200	975:987	IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200	946:987	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	4	15	theme	HMW	747:749	arg1	fraction					737:744	rhinoprolycan fraction	723:744	rhinoprolycan fraction (HMW & MMW)	723:756	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	15	theme	HMW	747:749	arg1	MMW					753:755	HMW & MMW	747:755	HMW & MMW	747:755	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	5	16	theme	IL-5	918:921	arg1	CWE					913:915	CWE	913:915	CWE (IL-5: 12.078 ± 1.225)	913:938	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	16	theme	IL-5	918:921	arg1	±					931:931	IL-5: 12.078 ± 1.225	918:937	IL-5: 12.078 ± 1.225	918:937	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	4	17	dep	chain	812:816	arg1	1,2,4,6-Glcp					855:866	1,2,4,6-Glcp	855:866	1,2,4,6-Glcp	855:866	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	17	dep	chain	812:816	arg1	1,3,6-Glcp					843:852	1,3,6-Glcp	843:852	1,3,6-Glcp	843:852	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	17	dep	chain	812:816	arg1	1,4,6-Glcp					831:840	1,4,6-Glcp	831:840	1,4,6-Glcp	831:840	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	17	dep	chain	812:816	arg1	1,3,6-Glcp					819:828	1,3,6-Glcp	819:828	1,3,6-Glcp	819:828	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	6	18	theme	Further	1158:1164	arg1	studies					1174:1180	Further in vivo studies	1158:1180	Further in vivo studies	1158:1180	Further in vivo studies are needed to fully understand the immunomodulatory effects of TM02®.					
33389874	5	19	dep	IL-6	946:949	arg1	±					958:958	7.297 ± 0.338	952:964	IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200	946:987	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	19	dep	IL-6	946:949	arg1	TIMP-1					967:972	TIMP-1	967:972	IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200	946:987	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	19	dep	IL-6	946:949	arg1	0.200					983:987	3.358 ± 0.200	975:987	IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200	946:987	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	4	20	theme	rhinoprolycan	723:735	arg1	fraction					737:744	rhinoprolycan fraction	723:744	rhinoprolycan fraction (HMW & MMW)	723:756	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	20	theme	rhinoprolycan	723:735	arg1	MMW					753:755	HMW & MMW	747:755	HMW & MMW	747:755	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	0	21	theme	Immunomodulating	4:19	arg1	Properties					21:30	The Immunomodulating Properties	0:30	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom	0:63	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	0	21	theme	Immunomodulating	4:19	arg1	Cultivar					92:99	Lignosus rhinocerus TM02® Cultivar	66:99	Lignosus rhinocerus TM02® Cultivar (Agaricomycetes)	66:116	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	0	21	theme	Immunomodulating	4:19	arg1	Composition					150:160	Its Associated Carbohydrate Composition	122:160	Its Associated Carbohydrate Composition	122:160	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	3	22	from	expression	544:553	arg1	RAW					623:625	L. rhinocerus (TM02®) cultivar treated RAW 264.7	584:631	L. rhinocerus (TM02®) cultivar treated RAW 264.7	584:631	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	4	23	theme	branched	803:810	arg1	chain					812:816	branched chain	803:816	branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp)	803:867	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	1	24	theme	Lignosus	190:197	arg1	rhinocerus					199:208	Lignosus rhinocerus	190:208	Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes	190:266	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes have the capabilities to modulate the immune system.					
33389874	5	25	theme	Cytokines	870:878	arg1	profile					880:886	Cytokines profile	870:886	Cytokines profile	870:886	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	26	theme	7.573	1081:1085	arg1	±					1087:1087	7.573 ± 0.088	1081:1093	MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088	1051:1093	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	4	27	theme	1,6-Glcp	781:788	arg1	backbone					790:797	1,6-Glcp backbone	781:797	1,6-Glcp backbone	781:797	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	2	28	theme	anti-inflammatory	348:364	arg1	properties					366:375	antitumor and anti-inflammatory properties	334:375	antitumor and anti-inflammatory properties	334:375	It possesses antitumor and anti-inflammatory properties and is commonly used in Southeast Asia and Southern China to alleviate illness.					
33389874	2	29	theme	Southern	420:427	arg1	China					429:433	Southern China	420:433	Southern China	420:433	It possesses antitumor and anti-inflammatory properties and is commonly used in Southeast Asia and Southern China to alleviate illness.					
33389874	6	30	theme	TM02®	1245:1249	arg1	effects					1234:1240	the immunomodulatory effects	1213:1240	the immunomodulatory effects of TM02®	1213:1249	Further in vivo studies are needed to fully understand the immunomodulatory effects of TM02®.					
33389874	2	31	used	used	393:396	arg2	It					321:322	It	321:322	It	321:322	It possesses antitumor and anti-inflammatory properties and is commonly used in Southeast Asia and Southern China to alleviate illness.					
33389874	0	32	dep	Lignosus	66:73	arg1	rhinocerus					75:84	rhinocerus	75:84	rhinocerus	75:84	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	3	33	theme	polysaccharides	520:534	arg1	composition					505:515	composition	505:515	composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7	505:631	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	5	34	from	LMW	1046:1048	arg1	involvement					1109:1119	possible involvement	1100:1119	possible involvement of NF-κB and MAPK signaling pathway	1100:1155	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	34	from	LMW	1046:1048	arg1	upregulation					895:906	upregulation	895:906	upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088)	895:1094	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	35	dep	MIP-2	1051:1055	arg1	TIMP-1					1073:1078	TIMP-1	1073:1078	MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088	1051:1093	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	35	dep	MIP-2	1051:1055	arg1	±					1087:1087	7.573 ± 0.088	1081:1093	MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088	1051:1093	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	35	dep	MIP-2	1051:1055	arg1	0.416					1066:1070	3.495 ± 0.416	1058:1070	MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088	1051:1093	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	2	36	theme	antitumor	334:342	arg1	properties					366:375	antitumor and anti-inflammatory properties	334:375	antitumor and anti-inflammatory properties	334:375	It possesses antitumor and anti-inflammatory properties and is commonly used in Southeast Asia and Southern China to alleviate illness.					
33389874	0	37	theme	Milk	41:44	arg1	Mushroom					56:63	Tiger Milk Medicinal Mushroom	35:63	Tiger Milk Medicinal Mushroom	35:63	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	2	38	contain	possesses	324:332	arg1	It					321:322	It	321:322	It	321:322	It possesses antitumor and anti-inflammatory properties and is commonly used in Southeast Asia and Southern China to alleviate illness.					
33389874	2	38	contain	possesses	324:332	arg2	properties					366:375	antitumor and anti-inflammatory properties	334:375	antitumor and anti-inflammatory properties	334:375	It possesses antitumor and anti-inflammatory properties and is commonly used in Southeast Asia and Southern China to alleviate illness.					
33389874	0	39	theme	Carbohydrate	137:148	arg1	Properties					21:30	The Immunomodulating Properties	0:30	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom	0:63	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	0	39	theme	Carbohydrate	137:148	arg1	Composition					150:160	Its Associated Carbohydrate Composition	122:160	Its Associated Carbohydrate Composition	122:160	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	1	40	located	found	181:185	arg2	compounds					171:179	Natural compounds	163:179	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes	163:266	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes have the capabilities to modulate the immune system.					
33389874	1	40	located	found	181:185	arg1	rhinocerus					199:208	Lignosus rhinocerus	190:208	Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes	190:266	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes have the capabilities to modulate the immune system.					
33389874	5	41	theme	IL-5	996:999	arg1	MMW					991:993	MMW	991:993	MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053)	991:1039	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	41	theme	IL-5	996:999	arg1	±					1009:1009	IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053	996:1038	IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053	996:1038	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	3	42	theme	immunomodulating	476:491	arg1	properties					493:502	its immunomodulating properties	472:502	its immunomodulating properties	472:502	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	0	43	theme	Tiger	35:39	arg1	Mushroom					56:63	Tiger Milk Medicinal Mushroom	35:63	Tiger Milk Medicinal Mushroom	35:63	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	5	44	dep	LMW	1046:1048	arg1	MIP-2					1051:1055	MIP-2	1051:1055	MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088	1051:1093	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	0	45	theme	Associated	126:135	arg1	Properties					21:30	The Immunomodulating Properties	0:30	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom	0:63	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	0	45	theme	Associated	126:135	arg1	Composition					150:160	Its Associated Carbohydrate Composition	122:160	Its Associated Carbohydrate Composition	122:160	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	5	46	theme	MAPK	1134:1137	arg1	pathway					1149:1155	NF-κB and MAPK signaling pathway	1124:1155	pathway	1149:1155	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	47	from	CWE	913:915	arg1	involvement					1109:1119	possible involvement	1100:1119	possible involvement of NF-κB and MAPK signaling pathway	1100:1155	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	47	from	CWE	913:915	arg1	upregulation					895:906	upregulation	895:906	upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088)	895:1094	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	1	48	theme	polysaccharide-protein	235:256	arg1	complexes					258:266	polysaccharide-protein complexes	235:266	polysaccharide-protein complexes	235:266	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes have the capabilities to modulate the immune system.					
33389874	0	49	theme	Mushroom	56:63	arg1	Properties					21:30	The Immunomodulating Properties	0:30	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom	0:63	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	0	49	theme	Mushroom	56:63	arg1	Cultivar					92:99	Lignosus rhinocerus TM02® Cultivar	66:99	Lignosus rhinocerus TM02® Cultivar (Agaricomycetes)	66:116	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	0	49	theme	Mushroom	56:63	arg1	Composition					150:160	Its Associated Carbohydrate Composition	122:160	Its Associated Carbohydrate Composition	122:160	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	1	50	contain	have	268:271	arg2	capabilities					277:288	the capabilities	273:288	the capabilities to modulate the immune system	273:318	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes have the capabilities to modulate the immune system.					
33389874	1	50	contain	have	268:271	arg1	compounds					171:179	Natural compounds	163:179	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes	163:266	Natural compounds found in Lignosus rhinocerus like polysaccharides and polysaccharide-protein complexes have the capabilities to modulate the immune system.					
33389874	5	51	dep	HMW	941:943	arg1	IL-6					946:949	IL-6	946:949	IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200	946:987	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	0	52	theme	Medicinal	46:54	arg1	Mushroom					56:63	Tiger Milk Medicinal Mushroom	35:63	Tiger Milk Medicinal Mushroom	35:63	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	3	53	from	polysaccharides	520:534	arg1	RAW					623:625	L. rhinocerus (TM02®) cultivar treated RAW 264.7	584:631	L. rhinocerus (TM02®) cultivar treated RAW 264.7	584:631	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	3	54	theme	cytokines/chemokines	558:577	arg1	polysaccharides					520:534	polysaccharides	520:534	polysaccharides	520:534	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	3	54	theme	cytokines/chemokines	558:577	arg1	expression					544:553	the expression	540:553	the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7	540:631	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	3	55	from	RAW	623:625	arg1	cytokines/chemokines					558:577	cytokines/chemokines	558:577	cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7	558:631	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	3	55	from	RAW	623:625	arg1	polysaccharides					520:534	polysaccharides	520:534	polysaccharides	520:534	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	3	55	from	RAW	623:625	arg1	expression					544:553	the expression	540:553	the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7	540:631	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	4	56	contain	contains	669:676	arg1	CWE					665:667	CWE	665:667	CWE	665:667	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	56	contain	contains	669:676	arg2	polysaccharides					685:699	linear polysaccharides	678:699	linear polysaccharides with 1,4-linkages	678:717	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	6	57	theme	immunomodulatory	1217:1232	arg1	effects					1234:1240	the immunomodulatory effects	1213:1240	the immunomodulatory effects of TM02®	1213:1249	Further in vivo studies are needed to fully understand the immunomodulatory effects of TM02®.					
33389874	5	58	theme	NF-κB	1124:1128	arg1	pathway					1149:1155	NF-κB and MAPK signaling pathway	1124:1155	pathway	1149:1155	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	3	59	theme	expression	544:553	arg1	composition					505:515	composition	505:515	composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7	505:631	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	5	60	theme	7.297	952:956	arg1	±					958:958	7.297 ± 0.338	952:964	IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200	946:987	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	0	61	theme	Lignosus	66:73	arg1	Properties					21:30	The Immunomodulating Properties	0:30	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom	0:63	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	0	61	theme	Lignosus	66:73	arg1	Agaricomycetes					102:115	Agaricomycetes	102:115	Agaricomycetes	102:115	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	0	61	theme	Lignosus	66:73	arg1	Cultivar					92:99	Lignosus rhinocerus TM02® Cultivar	66:99	Lignosus rhinocerus TM02® Cultivar (Agaricomycetes)	66:116	The Immunomodulating Properties of Tiger Milk Medicinal Mushroom, Lignosus rhinocerus TM02® Cultivar (Agaricomycetes) and Its Associated Carbohydrate Composition.					
33389874	4	62	with	polysaccharides	685:699	arg1	1,4-linkages					706:717	1,4-linkages	706:717	1,4-linkages	706:717	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	3	63	theme	rhinocerus	587:596	arg1	RAW					623:625	L. rhinocerus (TM02®) cultivar treated RAW 264.7	584:631	L. rhinocerus (TM02®) cultivar treated RAW 264.7	584:631	To investigate its immunomodulating properties, composition of polysaccharides and the expression of cytokines/chemokines from L. rhinocerus (TM02®) cultivar treated RAW 264.7 were explored.					
33389874	5	64	dep	±	1009:1009	arg1	TIMP-1					1018:1023	TIMP-1	1018:1023	IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053	996:1038	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	64	dep	±	1009:1009	arg1	±					1032:1032	1.747 ± 0.053	1026:1038	IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053	996:1038	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	4	65	theme	linear	678:683	arg1	polysaccharides					685:699	linear polysaccharides	678:699	linear polysaccharides with 1,4-linkages	678:717	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	66	theme	&	751:751	arg1	fraction					737:744	rhinoprolycan fraction	723:744	rhinoprolycan fraction (HMW & MMW)	723:756	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	4	66	theme	&	751:751	arg1	MMW					753:755	HMW & MMW	747:755	HMW & MMW	747:755	It was revealed, CWE contains linear polysaccharides with 1,4-linkages and rhinoprolycan fraction (HMW & MMW) possesses 1,4-Glcp and 1,6-Glcp backbone and branched chain (1,3,6-Glcp, 1,4,6-Glcp, 1,3,6-Glcp, 1,2,4,6-Glcp).					
33389874	5	67	theme	pathway	1149:1155	arg1	involvement					1109:1119	possible involvement	1100:1119	possible involvement of NF-κB and MAPK signaling pathway	1100:1155	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	67	theme	pathway	1149:1155	arg1	upregulation					895:906	upregulation	895:906	upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088)	895:1094	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
33389874	5	68	theme	signaling	1139:1147	arg1	pathway					1149:1155	NF-κB and MAPK signaling pathway	1124:1155	pathway	1149:1155	Cytokines profile showed upregulation from CWE (IL-5: 12.078 ± 1.225), HMW (IL-6: 7.297 ± 0.338; TIMP-1: 3.358 ± 0.200), MMW (IL-5: 15.412 ± 5.823; TIMP-1: 1.747 ± 0.053), and LMW (MIP-2: 3.495 ± 0.416; TIMP-1: 7.573 ± 0.088) and possible involvement of NF-κB and MAPK signaling pathway.					
34664583	0	0	theme	protective	82:91	arg1	effects					93:99	the protective effects	78:99	the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis	78:166	Gut microbiota-dependent catabolites of tryptophan play a predominant role in the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis.					
34664583	5	1	theme	gut	894:896	arg1	dysbiosis					909:917	gut microbiota dysbiosis	894:917	gut microbiota dysbiosis caused by DSS	894:931	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	1	2	theme	barrier	173:179	arg1	dysfunction					181:191	Gut barrier dysfunction	169:191	Gut barrier dysfunction	169:191	Gut barrier dysfunction is triggered by gut microbiota dysbiosis that is closely associated with ulcerative colitis.					
34664583	7	3	theme	gut	1546:1548	arg1	function					1558:1565	gut barrier function	1546:1565	gut barrier function	1546:1565	These findings highlight the protective effects of TPS against ulcerative colitis by modulating the gut microbiota and improving microbial metabolites and gut barrier function.					
34664583	2	4	theme	plant	381:385	arg1	polysaccharides					387:401	the non-digestively colon-targeted plant polysaccharides	346:401	the non-digestively colon-targeted plant polysaccharides	346:401	Recently, more attention has been devoted to the ability of the non-digestively colon-targeted plant polysaccharides to regulate the function and composition of the intestinal microbiota.					
34664583	6	5	theme	AhR	1337:1339	arg1	activation					1323:1332	the activation	1319:1332	the activation of AhR to upregulate epithelial tight junction proteins	1319:1388	Expectedly, TPS was found to exert its gut barrier functions through the activation of AhR to upregulate epithelial tight junction proteins.					
34664583	5	6	theme	acid	1123:1126	arg1	expressions					1181:1191	the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions	1075:1191	the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions	1075:1191	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	4	7	theme	TPS	660:662	arg1	administration					664:677	TPS administration	660:677	TPS administration	660:677	The results revealed that TPS administration could greatly improve the pathological phenotype, gut barrier disruption and colon inflammation in colitis mice.					
34664583	7	8	theme	microbial	1520:1528	arg1	metabolites					1530:1540	microbial metabolites	1520:1540	microbial metabolites	1520:1540	These findings highlight the protective effects of TPS against ulcerative colitis by modulating the gut microbiota and improving microbial metabolites and gut barrier function.					
34664583	2	9	theme	polysaccharides	387:401	arg1	ability					335:341	the ability	331:341	the ability of the non-digestively colon-targeted plant polysaccharides to regulate the function and composition of the intestinal microbiota	331:471	Recently, more attention has been devoted to the ability of the non-digestively colon-targeted plant polysaccharides to regulate the function and composition of the intestinal microbiota.					
34664583	4	10	from	phenotype	718:726	arg1	mice					786:789	colitis mice	778:789	colitis mice	778:789	The results revealed that TPS administration could greatly improve the pathological phenotype, gut barrier disruption and colon inflammation in colitis mice.					
34664583	0	11	theme	polysaccharides	113:127	arg1	effects					93:99	the protective effects	78:99	the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis	78:166	Gut microbiota-dependent catabolites of tryptophan play a predominant role in the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis.					
34664583	2	12	theme	colon-targeted	366:379	arg1	polysaccharides					387:401	the non-digestively colon-targeted plant polysaccharides	346:401	the non-digestively colon-targeted plant polysaccharides	346:401	Recently, more attention has been devoted to the ability of the non-digestively colon-targeted plant polysaccharides to regulate the function and composition of the intestinal microbiota.					
34664583	5	13	theme	microbiota	898:907	arg1	dysbiosis					909:917	gut microbiota dysbiosis	894:917	gut microbiota dysbiosis caused by DSS	894:931	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	3	14	theme	prophylactic	501:512	arg1	capacity					514:521	the prophylactic capacity	497:521	the prophylactic capacity of turmeric polysaccharides (TPS) to ameliorate dextran sulfate sodium (DSS)-induced gut microbiota imbalance	497:631	Here, we first studied the prophylactic capacity of turmeric polysaccharides (TPS) to ameliorate dextran sulfate sodium (DSS)-induced gut microbiota imbalance.					
34664583	5	15	theme	rRNA-based	830:839	arg1	analysis					852:859	16S rRNA-based microbiota analysis	826:859	16S rRNA-based microbiota analysis	826:859	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	0	16	theme	turmeric	104:111	arg1	polysaccharides					113:127	turmeric polysaccharides	104:127	turmeric polysaccharides	104:127	Gut microbiota-dependent catabolites of tryptophan play a predominant role in the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis.					
34664583	0	17	theme	microbiota-dependent	4:23	arg1	catabolites					25:35	Gut microbiota-dependent catabolites	0:35	Gut microbiota-dependent catabolites of tryptophan	0:49	Gut microbiota-dependent catabolites of tryptophan play a predominant role in the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis.					
34664583	6	18	theme	gut	1289:1291	arg1	functions					1301:1309	its gut barrier functions	1285:1309	its gut barrier functions	1285:1309	Expectedly, TPS was found to exert its gut barrier functions through the activation of AhR to upregulate epithelial tight junction proteins.					
34664583	7	19	theme	barrier	1550:1556	arg1	function					1558:1565	gut barrier function	1546:1565	gut barrier function	1546:1565	These findings highlight the protective effects of TPS against ulcerative colitis by modulating the gut microbiota and improving microbial metabolites and gut barrier function.					
34664583	0	20	theme	Gut	0:2	arg1	catabolites					25:35	Gut microbiota-dependent catabolites	0:35	Gut microbiota-dependent catabolites of tryptophan	0:49	Gut microbiota-dependent catabolites of tryptophan play a predominant role in the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis.					
34664583	3	21	theme	-induced	599:606	arg1	imbalance					623:631	dextran sulfate sodium (DSS)-induced gut microbiota imbalance	571:631	dextran sulfate sodium (DSS)-induced gut microbiota imbalance	571:631	Here, we first studied the prophylactic capacity of turmeric polysaccharides (TPS) to ameliorate dextran sulfate sodium (DSS)-induced gut microbiota imbalance.					
34664583	1	22	theme	gut	209:211	arg1	dysbiosis					224:232	gut microbiota dysbiosis	209:232	gut microbiota dysbiosis that is closely associated with ulcerative colitis	209:283	Gut barrier dysfunction is triggered by gut microbiota dysbiosis that is closely associated with ulcerative colitis.					
34664583	7	23	theme	TPS	1442:1444	arg1	effects					1431:1437	the protective effects	1416:1437	the protective effects of TPS against ulcerative colitis	1416:1471	These findings highlight the protective effects of TPS against ulcerative colitis by modulating the gut microbiota and improving microbial metabolites and gut barrier function.					
34664583	7	24	theme	gut	1491:1493	arg1	microbiota					1495:1504	the gut microbiota	1487:1504	the gut microbiota	1487:1504	These findings highlight the protective effects of TPS against ulcerative colitis by modulating the gut microbiota and improving microbial metabolites and gut barrier function.					
34664583	0	25	theme	DSS-induced	137:147	arg1	colitis					160:166	DSS-induced ulcerative colitis	137:166	DSS-induced ulcerative colitis	137:166	Gut microbiota-dependent catabolites of tryptophan play a predominant role in the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis.					
34664583	7	26	theme	protective	1420:1429	arg1	effects					1431:1437	the protective effects	1416:1437	the protective effects of TPS against ulcerative colitis	1416:1471	These findings highlight the protective effects of TPS against ulcerative colitis by modulating the gut microbiota and improving microbial metabolites and gut barrier function.					
34664583	6	27	theme	barrier	1293:1299	arg1	functions					1301:1309	its gut barrier functions	1285:1309	its gut barrier functions	1285:1309	Expectedly, TPS was found to exert its gut barrier functions through the activation of AhR to upregulate epithelial tight junction proteins.					
34664583	2	28	theme	microbiota	462:471	arg1	composition					432:442	composition	432:442	composition	432:442	Recently, more attention has been devoted to the ability of the non-digestively colon-targeted plant polysaccharides to regulate the function and composition of the intestinal microbiota.					
34664583	2	28	theme	microbiota	462:471	arg1	function					419:426	function	419:426	function	419:426	Recently, more attention has been devoted to the ability of the non-digestively colon-targeted plant polysaccharides to regulate the function and composition of the intestinal microbiota.					
34664583	4	29	theme	colitis	778:784	arg1	mice					786:789	colitis mice	778:789	colitis mice	778:789	The results revealed that TPS administration could greatly improve the pathological phenotype, gut barrier disruption and colon inflammation in colitis mice.					
34664583	5	30	theme	ligand	1142:1147	arg1	AhR					1176:1178	AhR	1176:1178	AhR	1176:1178	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	30	theme	ligand	1142:1147	arg1	receptor					1166:1173	its ligand aryl hydrocarbon receptor	1138:1173	its ligand aryl hydrocarbon receptor (AhR)	1138:1179	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	4	31	theme	barrier	733:739	arg1	disruption					741:750	gut barrier disruption	729:750	gut barrier disruption	729:750	The results revealed that TPS administration could greatly improve the pathological phenotype, gut barrier disruption and colon inflammation in colitis mice.					
34664583	2	32	theme	intestinal	451:460	arg1	microbiota					462:471	the intestinal microbiota	447:471	the intestinal microbiota	447:471	Recently, more attention has been devoted to the ability of the non-digestively colon-targeted plant polysaccharides to regulate the function and composition of the intestinal microbiota.					
34664583	5	33	theme	16S	826:828	arg1	analysis					852:859	16S rRNA-based microbiota analysis	826:859	16S rRNA-based microbiota analysis	826:859	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	3	34	theme	polysaccharides	535:549	arg1	capacity					514:521	the prophylactic capacity	497:521	the prophylactic capacity of turmeric polysaccharides (TPS) to ameliorate dextran sulfate sodium (DSS)-induced gut microbiota imbalance	497:631	Here, we first studied the prophylactic capacity of turmeric polysaccharides (TPS) to ameliorate dextran sulfate sodium (DSS)-induced gut microbiota imbalance.					
34664583	5	35	theme	aryl	1149:1152	arg1	AhR					1176:1178	AhR	1176:1178	AhR	1176:1178	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	35	theme	aryl	1149:1152	arg1	receptor					1166:1173	its ligand aryl hydrocarbon receptor	1138:1173	its ligand aryl hydrocarbon receptor (AhR)	1138:1179	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	36	theme	catabolite	1096:1105	arg1	IAA					1129:1131	IAA	1129:1131	IAA	1129:1131	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	36	theme	catabolite	1096:1105	arg1	acid					1123:1126	the cecal tryptophan catabolite indole-3-acetic acid	1075:1126	the cecal tryptophan catabolite indole-3-acetic acid (IAA)	1075:1132	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	4	37	theme	pathological	705:716	arg1	phenotype					718:726	the pathological phenotype	701:726	the pathological phenotype	701:726	The results revealed that TPS administration could greatly improve the pathological phenotype, gut barrier disruption and colon inflammation in colitis mice.					
34664583	0	38	theme	tryptophan	40:49	arg1	catabolites					25:35	Gut microbiota-dependent catabolites	0:35	Gut microbiota-dependent catabolites of tryptophan	0:49	Gut microbiota-dependent catabolites of tryptophan play a predominant role in the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis.					
34664583	5	39	theme	TPS	1219:1221	arg1	treatment					1223:1231	TPS treatment	1219:1231	TPS treatment	1219:1231	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	0	40	theme	ulcerative	149:158	arg1	colitis					160:166	DSS-induced ulcerative colitis	137:166	DSS-induced ulcerative colitis	137:166	Gut microbiota-dependent catabolites of tryptophan play a predominant role in the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis.					
34664583	5	41	theme	hydrocarbon	1154:1164	arg1	AhR					1176:1178	AhR	1176:1178	AhR	1176:1178	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	41	theme	hydrocarbon	1154:1164	arg1	receptor					1166:1173	its ligand aryl hydrocarbon receptor	1138:1173	its ligand aryl hydrocarbon receptor (AhR)	1138:1179	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	42	theme	indole-3-acetic	1107:1121	arg1	IAA					1129:1131	IAA	1129:1131	IAA	1129:1131	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	42	theme	indole-3-acetic	1107:1121	arg1	acid					1123:1126	the cecal tryptophan catabolite indole-3-acetic acid	1075:1126	the cecal tryptophan catabolite indole-3-acetic acid (IAA)	1075:1132	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	0	43	theme	predominant	58:68	arg1	role					70:73	a predominant role	56:73	a predominant role	56:73	Gut microbiota-dependent catabolites of tryptophan play a predominant role in the protective effects of turmeric polysaccharides against DSS-induced ulcerative colitis.					
34664583	5	44	theme	receptor	1166:1173	arg1	expressions					1181:1191	the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions	1075:1191	the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions	1075:1191	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	3	45	theme	gut	608:610	arg1	imbalance					623:631	dextran sulfate sodium (DSS)-induced gut microbiota imbalance	571:631	dextran sulfate sodium (DSS)-induced gut microbiota imbalance	571:631	Here, we first studied the prophylactic capacity of turmeric polysaccharides (TPS) to ameliorate dextran sulfate sodium (DSS)-induced gut microbiota imbalance.					
34664583	6	46	theme	tight	1366:1370	arg1	proteins					1381:1388	epithelial tight junction proteins	1355:1388	epithelial tight junction proteins	1355:1388	Expectedly, TPS was found to exert its gut barrier functions through the activation of AhR to upregulate epithelial tight junction proteins.					
34664583	3	47	theme	turmeric	526:533	arg1	TPS					552:554	TPS	552:554	TPS	552:554	Here, we first studied the prophylactic capacity of turmeric polysaccharides (TPS) to ameliorate dextran sulfate sodium (DSS)-induced gut microbiota imbalance.					
34664583	3	47	theme	turmeric	526:533	arg1	polysaccharides					535:549	turmeric polysaccharides	526:549	turmeric polysaccharides (TPS)	526:555	Here, we first studied the prophylactic capacity of turmeric polysaccharides (TPS) to ameliorate dextran sulfate sodium (DSS)-induced gut microbiota imbalance.					
34664583	6	48	theme	junction	1372:1379	arg1	proteins					1381:1388	epithelial tight junction proteins	1355:1388	epithelial tight junction proteins	1355:1388	Expectedly, TPS was found to exert its gut barrier functions through the activation of AhR to upregulate epithelial tight junction proteins.					
34664583	7	49	theme	ulcerative	1454:1463	arg1	colitis					1465:1471	ulcerative colitis	1454:1471	ulcerative colitis	1454:1471	These findings highlight the protective effects of TPS against ulcerative colitis by modulating the gut microbiota and improving microbial metabolites and gut barrier function.					
34664583	3	50	theme	microbiota	612:621	arg1	imbalance					623:631	dextran sulfate sodium (DSS)-induced gut microbiota imbalance	571:631	dextran sulfate sodium (DSS)-induced gut microbiota imbalance	571:631	Here, we first studied the prophylactic capacity of turmeric polysaccharides (TPS) to ameliorate dextran sulfate sodium (DSS)-induced gut microbiota imbalance.					
34664583	2	51	theme	non-digestively	350:364	arg1	polysaccharides					387:401	the non-digestively colon-targeted plant polysaccharides	346:401	the non-digestively colon-targeted plant polysaccharides	346:401	Recently, more attention has been devoted to the ability of the non-digestively colon-targeted plant polysaccharides to regulate the function and composition of the intestinal microbiota.					
34664583	5	52	theme	colitis	1236:1242	arg1	mice					1244:1247	colitis mice	1236:1247	colitis mice	1236:1247	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	4	53	theme	colon	756:760	arg1	inflammation					762:773	colon inflammation	756:773	colon inflammation	756:773	The results revealed that TPS administration could greatly improve the pathological phenotype, gut barrier disruption and colon inflammation in colitis mice.					
34664583	2	54	dep	function	419:426	arg1	the					415:417	the	415:417	the	415:417	Recently, more attention has been devoted to the ability of the non-digestively colon-targeted plant polysaccharides to regulate the function and composition of the intestinal microbiota.					
34664583	5	55	theme	targeted	801:808	arg1	metabolomics					810:821	targeted metabolomics	801:821	targeted metabolomics	801:821	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	4	56	from	inflammation	762:773	arg1	mice					786:789	colitis mice	778:789	colitis mice	778:789	The results revealed that TPS administration could greatly improve the pathological phenotype, gut barrier disruption and colon inflammation in colitis mice.					
34664583	6	57	theme	epithelial	1355:1364	arg1	proteins					1381:1388	epithelial tight junction proteins	1355:1388	epithelial tight junction proteins	1355:1388	Expectedly, TPS was found to exert its gut barrier functions through the activation of AhR to upregulate epithelial tight junction proteins.					
34664583	5	58	theme	probiotics	973:982	arg1	abundance					960:968	the abundance	956:968	the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice	956:1247	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	59	theme	tryptophan	1000:1009	arg1	metabolism					1011:1020	tryptophan metabolism	1000:1020	tryptophan metabolism	1000:1020	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	60	theme	cecal	1079:1083	arg1	IAA					1129:1131	IAA	1129:1131	IAA	1129:1131	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	60	theme	cecal	1079:1083	arg1	acid					1123:1126	the cecal tryptophan catabolite indole-3-acetic acid	1075:1126	the cecal tryptophan catabolite indole-3-acetic acid (IAA)	1075:1132	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	1	61	theme	microbiota	213:222	arg1	dysbiosis					224:232	gut microbiota dysbiosis	209:232	gut microbiota dysbiosis that is closely associated with ulcerative colitis	209:283	Gut barrier dysfunction is triggered by gut microbiota dysbiosis that is closely associated with ulcerative colitis.					
34664583	4	62	theme	gut	729:731	arg1	disruption					741:750	gut barrier disruption	729:750	gut barrier disruption	729:750	The results revealed that TPS administration could greatly improve the pathological phenotype, gut barrier disruption and colon inflammation in colitis mice.					
34664583	5	63	theme	microbiota	841:850	arg1	analysis					852:859	16S rRNA-based microbiota analysis	826:859	16S rRNA-based microbiota analysis	826:859	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	64	theme	tryptophan	1085:1094	arg1	IAA					1129:1131	IAA	1129:1131	IAA	1129:1131	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	5	64	theme	tryptophan	1085:1094	arg1	acid					1123:1126	the cecal tryptophan catabolite indole-3-acetic acid	1075:1126	the cecal tryptophan catabolite indole-3-acetic acid (IAA)	1075:1132	Besides, targeted metabolomics or 16S rRNA-based microbiota analysis demonstrated that TPS alleviated gut microbiota dysbiosis caused by DSS, especially increasing the abundance of probiotics associated with tryptophan metabolism, such as Lactobacillus and Clostridia-UCG-014, where the cecal tryptophan catabolite indole-3-acetic acid (IAA) and its ligand aryl hydrocarbon receptor (AhR) expressions were sharply increased by TPS treatment in colitis mice.					
34664583	1	65	theme	Gut	169:171	arg1	dysfunction					181:191	Gut barrier dysfunction	169:191	Gut barrier dysfunction	169:191	Gut barrier dysfunction is triggered by gut microbiota dysbiosis that is closely associated with ulcerative colitis.					
34664583	4	66	from	disruption	741:750	arg1	mice					786:789	colitis mice	778:789	colitis mice	778:789	The results revealed that TPS administration could greatly improve the pathological phenotype, gut barrier disruption and colon inflammation in colitis mice.					
34664583	1	67	theme	ulcerative	266:275	arg1	colitis					277:283	ulcerative colitis	266:283	ulcerative colitis	266:283	Gut barrier dysfunction is triggered by gut microbiota dysbiosis that is closely associated with ulcerative colitis.					
34973795	5	0	theme	mild	740:743	arg1	temperature					745:755	mild temperature	740:755	mild temperature	740:755	The results revealed that the suitable acidity of DESs and mild temperature effectively avoided the over-degradation of hemicelluloses.					
34973795	8	1	theme	sustainable	1144:1154	arg1	route					1156:1160	a practical and sustainable route	1128:1160	a practical and sustainable route for hemicellulose extraction in acidic condition	1128:1209	The novel and eco-friendly processing offers a practical and sustainable route for hemicellulose extraction in acidic condition.					
34973795	1	2	theme	promising	139:147	arg1	solvents					119:126	Acidic deep eutectic solvents	98:126	Acidic deep eutectic solvents (DESs)	98:133	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	1	2	theme	promising	139:147	arg1	media					149:153	promising media	139:153	promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall	139:275	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	1	3	theme	efficient	233:241	arg1	deconstruction					243:256	efficient deconstruction	233:256	efficient deconstruction of plant cell wall	233:275	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	5	4	theme	temperature	745:755	arg1	acidity					720:726	the suitable acidity	707:726	the suitable acidity of DESs and mild temperature	707:755	The results revealed that the suitable acidity of DESs and mild temperature effectively avoided the over-degradation of hemicelluloses.					
34973795	4	5	theme	xylan	674:678	arg1	solubility					660:669	the high solubility	651:669	the high solubility of xylan	651:678	Oxalic acid (OA)-based BDESs were preferred for corncob processing due to the high solubility of xylan.					
34973795	4	6	theme	Oxalic	577:582	arg1	OA					590:591	OA	590:591	OA	590:591	Oxalic acid (OA)-based BDESs were preferred for corncob processing due to the high solubility of xylan.					
34973795	4	6	theme	Oxalic	577:582	arg1	acid					584:587	Oxalic acid	577:587	Oxalic acid (OA)-based BDESs	577:604	Oxalic acid (OA)-based BDESs were preferred for corncob processing due to the high solubility of xylan.					
34973795	2	7	from	lignocellulose	317:330	arg1	extraction					301:310	hemicellulose extraction	287:310	hemicellulose extraction from lignocellulose using acidic DESs	287:348	However, hemicellulose extraction from lignocellulose using acidic DESs remains a challenge.					
34973795	0	8	theme	hemicelluloses	82:95	arg1	fractionation					65:77	mild fractionation	60:77	mild fractionation of hemicelluloses	60:95	Novel, recyclable Brønsted acidic deep eutectic solvent for mild fractionation of hemicelluloses.					
34973795	3	9	theme	mild	539:542	arg1	fractionation					544:556	mild fractionation	539:556	mild fractionation of hemicelluloses	539:574	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	1	10	theme	plant	261:265	arg1	wall					272:275	plant cell wall	261:275	plant cell wall	261:275	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	8	11	theme	acidic	1194:1199	arg1	condition					1201:1209	acidic condition	1194:1209	acidic condition	1194:1209	The novel and eco-friendly processing offers a practical and sustainable route for hemicellulose extraction in acidic condition.					
34973795	3	12	theme	common	472:477	arg1	polyols					479:485	common polyols	472:485	common polyols	472:485	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	4	13	theme	acid	584:587	arg1	BDESs					600:604	Oxalic acid (OA)-based BDESs	577:604	Oxalic acid (OA)-based BDESs	577:604	Oxalic acid (OA)-based BDESs were preferred for corncob processing due to the high solubility of xylan.					
34973795	3	14	theme	Brønsted	395:402	arg1	BDESs					417:421	BDESs	417:421	BDESs	417:421	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	3	14	theme	Brønsted	395:402	arg1	DESs					411:414	Brønsted acidic DESs	395:414	novel and green Brønsted acidic DESs (BDESs)	379:422	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	3	15	theme	acidic	404:409	arg1	BDESs					417:421	BDESs	417:421	BDESs	417:421	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	3	15	theme	acidic	404:409	arg1	DESs					411:414	Brønsted acidic DESs	395:414	novel and green Brønsted acidic DESs (BDESs)	379:422	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	5	16	theme	hemicelluloses	801:814	arg1	over-degradation					781:796	the over-degradation	777:796	the over-degradation of hemicelluloses	777:814	The results revealed that the suitable acidity of DESs and mild temperature effectively avoided the over-degradation of hemicelluloses.					
34973795	8	17	theme	eco-friendly	1097:1108	arg1	processing					1110:1119	The novel and eco-friendly processing	1083:1119	The novel and eco-friendly processing	1083:1119	The novel and eco-friendly processing offers a practical and sustainable route for hemicellulose extraction in acidic condition.					
34973795	1	18	theme	lignin	159:164	arg1	valorization					166:177	lignin valorization	159:177	lignin valorization	159:177	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	3	19	theme	novel	379:383	arg1	BDESs					417:421	BDESs	417:421	BDESs	417:421	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	3	19	theme	novel	379:383	arg1	DESs					411:414	Brønsted acidic DESs	395:414	novel and green Brønsted acidic DESs (BDESs)	379:422	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	6	20	theme	hemicelluloses	883:896	arg1	composition					830:840	chemical composition	821:840	chemical composition	821:840	The chemical composition and structural features of the recovered hemicelluloses were investigated systematically.					
34973795	6	20	theme	hemicelluloses	883:896	arg1	features					857:864	structural features	846:864	structural features	846:864	The chemical composition and structural features of the recovered hemicelluloses were investigated systematically.					
34973795	5	21	theme	DESs	731:734	arg1	acidity					720:726	the suitable acidity	707:726	the suitable acidity of DESs and mild temperature	707:755	The results revealed that the suitable acidity of DESs and mild temperature effectively avoided the over-degradation of hemicelluloses.					
34973795	3	22	theme	hemicelluloses	561:574	arg1	fractionation					544:556	mild fractionation	539:556	mild fractionation of hemicelluloses	539:574	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	4	23	theme	high	655:658	arg1	solubility					660:669	the high solubility	651:669	the high solubility of xylan	651:678	Oxalic acid (OA)-based BDESs were preferred for corncob processing due to the high solubility of xylan.					
34973795	0	24	theme	Novel	0:4	arg1	Brønsted					18:25	Novel, recyclable Brønsted	0:25	Novel, recyclable Brønsted	0:25	Novel, recyclable Brønsted acidic deep eutectic solvent for mild fractionation of hemicelluloses.					
34973795	1	25	theme	cell	267:270	arg1	wall					272:275	plant cell wall	261:275	plant cell wall	261:275	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	7	26	theme	ethylene	948:955	arg1	EG					965:966	EG	965:966	EG	965:966	Moreover, after ethylene glycol (EG)-OA BDES was recycled and reused three times, the extraction still resulted in a satisfactory hemicellulose yield.					
34973795	7	26	theme	ethylene	948:955	arg1	glycol					957:962	ethylene glycol	948:962	ethylene glycol (EG)-OA BDES	948:975	Moreover, after ethylene glycol (EG)-OA BDES was recycled and reused three times, the extraction still resulted in a satisfactory hemicellulose yield.					
34973795	1	27	theme	wall	272:275	arg1	deconstruction					243:256	efficient deconstruction	233:256	efficient deconstruction of plant cell wall	233:275	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	8	28	theme	novel	1087:1091	arg1	processing					1110:1119	The novel and eco-friendly processing	1083:1119	The novel and eco-friendly processing	1083:1119	The novel and eco-friendly processing offers a practical and sustainable route for hemicellulose extraction in acidic condition.					
34973795	7	29	theme	glycol	957:962	arg1	BDES					972:975	ethylene glycol (EG)-OA BDES	948:975	ethylene glycol (EG)-OA BDES	948:975	Moreover, after ethylene glycol (EG)-OA BDES was recycled and reused three times, the extraction still resulted in a satisfactory hemicellulose yield.					
34973795	2	30	theme	hemicellulose	287:299	arg1	extraction					301:310	hemicellulose extraction	287:310	hemicellulose extraction from lignocellulose using acidic DESs	287:348	However, hemicellulose extraction from lignocellulose using acidic DESs remains a challenge.					
34973795	1	31	theme	cellulose	183:191	arg1	conversion					193:202	cellulose conversion	183:202	cellulose conversion	183:202	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	6	32	theme	recovered	873:881	arg1	hemicelluloses					883:896	the recovered hemicelluloses	869:896	the recovered hemicelluloses	869:896	The chemical composition and structural features of the recovered hemicelluloses were investigated systematically.					
34973795	1	33	theme	deep	105:108	arg1	DESs					129:132	DESs	129:132	DESs	129:132	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	1	33	theme	deep	105:108	arg1	solvents					119:126	Acidic deep eutectic solvents	98:126	Acidic deep eutectic solvents (DESs)	98:133	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	1	33	theme	deep	105:108	arg1	media					149:153	promising media	139:153	promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall	139:275	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	7	34	theme	-OA	968:970	arg1	BDES					972:975	ethylene glycol (EG)-OA BDES	948:975	ethylene glycol (EG)-OA BDES	948:975	Moreover, after ethylene glycol (EG)-OA BDES was recycled and reused three times, the extraction still resulted in a satisfactory hemicellulose yield.					
34973795	3	35	theme	organic	454:460	arg1	acids					462:466	natural organic acids	446:466	natural organic acids	446:466	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	0	36	theme	deep	34:37	arg1	solvent					48:54	deep eutectic solvent	34:54	deep eutectic solvent for mild fractionation of hemicelluloses	34:95	Novel, recyclable Brønsted acidic deep eutectic solvent for mild fractionation of hemicelluloses.					
34973795	7	37	theme	satisfactory	1049:1060	arg1	yield					1076:1080	a satisfactory hemicellulose yield	1047:1080	a satisfactory hemicellulose yield	1047:1080	Moreover, after ethylene glycol (EG)-OA BDES was recycled and reused three times, the extraction still resulted in a satisfactory hemicellulose yield.					
34973795	7	38	theme	hemicellulose	1062:1074	arg1	yield					1076:1080	a satisfactory hemicellulose yield	1047:1080	a satisfactory hemicellulose yield	1047:1080	Moreover, after ethylene glycol (EG)-OA BDES was recycled and reused three times, the extraction still resulted in a satisfactory hemicellulose yield.					
34973795	4	39	theme	corncob	625:631	arg1	processing					633:642	corncob processing	625:642	corncob processing due to the high solubility of xylan	625:678	Oxalic acid (OA)-based BDESs were preferred for corncob processing due to the high solubility of xylan.					
34973795	3	40	theme	green	389:393	arg1	BDESs					417:421	BDESs	417:421	BDESs	417:421	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	3	40	theme	green	389:393	arg1	DESs					411:414	Brønsted acidic DESs	395:414	novel and green Brønsted acidic DESs (BDESs)	379:422	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	1	41	theme	eutectic	110:117	arg1	DESs					129:132	DESs	129:132	DESs	129:132	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	1	41	theme	eutectic	110:117	arg1	solvents					119:126	Acidic deep eutectic solvents	98:126	Acidic deep eutectic solvents (DESs)	98:133	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	1	41	theme	eutectic	110:117	arg1	media					149:153	promising media	139:153	promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall	139:275	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	0	42	theme	eutectic	39:46	arg1	solvent					48:54	deep eutectic solvent	34:54	deep eutectic solvent for mild fractionation of hemicelluloses	34:95	Novel, recyclable Brønsted acidic deep eutectic solvent for mild fractionation of hemicelluloses.					
34973795	6	43	theme	structural	846:855	arg1	features					857:864	structural features	846:864	structural features	846:864	The chemical composition and structural features of the recovered hemicelluloses were investigated systematically.					
34973795	6	44	dep	composition	830:840	arg1	The					817:819	The	817:819	The	817:819	The chemical composition and structural features of the recovered hemicelluloses were investigated systematically.					
34973795	2	45	theme	acidic	338:343	arg1	DESs					345:348	acidic DESs	338:348	acidic DESs	338:348	However, hemicellulose extraction from lignocellulose using acidic DESs remains a challenge.					
34973795	8	46	theme	practical	1130:1138	arg1	route					1156:1160	a practical and sustainable route	1128:1160	a practical and sustainable route for hemicellulose extraction in acidic condition	1128:1209	The novel and eco-friendly processing offers a practical and sustainable route for hemicellulose extraction in acidic condition.					
34973795	8	47	theme	hemicellulose	1166:1178	arg1	extraction					1180:1189	hemicellulose extraction	1166:1189	hemicellulose extraction in acidic condition	1166:1209	The novel and eco-friendly processing offers a practical and sustainable route for hemicellulose extraction in acidic condition.					
34973795	1	48	from	conversion	193:202	arg1	deconstruction					243:256	efficient deconstruction	233:256	efficient deconstruction of plant cell wall	233:275	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	1	49	theme	Acidic	98:103	arg1	DESs					129:132	DESs	129:132	DESs	129:132	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	1	49	theme	Acidic	98:103	arg1	solvents					119:126	Acidic deep eutectic solvents	98:126	Acidic deep eutectic solvents (DESs)	98:133	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	1	49	theme	Acidic	98:103	arg1	media					149:153	promising media	139:153	promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall	139:275	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	4	50	theme	-based	593:598	arg1	BDESs					600:604	Oxalic acid (OA)-based BDESs	577:604	Oxalic acid (OA)-based BDESs	577:604	Oxalic acid (OA)-based BDESs were preferred for corncob processing due to the high solubility of xylan.					
34973795	8	51	from	extraction	1180:1189	arg1	condition					1201:1209	acidic condition	1194:1209	acidic condition	1194:1209	The novel and eco-friendly processing offers a practical and sustainable route for hemicellulose extraction in acidic condition.					
34973795	1	52	from	valorization	166:177	arg1	deconstruction					243:256	efficient deconstruction	233:256	efficient deconstruction of plant cell wall	233:275	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	1	53	theme	good	217:220	arg1	ability					222:228	their good ability	211:228	their good ability	211:228	Acidic deep eutectic solvents (DESs) are promising media for lignin valorization and cellulose conversion due to their good ability in efficient deconstruction of plant cell wall.					
34973795	0	54	theme	mild	60:63	arg1	fractionation					65:77	mild fractionation	60:77	mild fractionation of hemicelluloses	60:95	Novel, recyclable Brønsted acidic deep eutectic solvent for mild fractionation of hemicelluloses.					
34973795	6	55	theme	chemical	821:828	arg1	composition					830:840	chemical composition	821:840	chemical composition	821:840	The chemical composition and structural features of the recovered hemicelluloses were investigated systematically.					
34973795	3	56	theme	natural	446:452	arg1	acids					462:466	natural organic acids	446:466	natural organic acids	446:466	Herein, novel and green Brønsted acidic DESs (BDESs) were synthesized from natural organic acids and common polyols and successively adopted to deconstruct corncob for mild fractionation of hemicelluloses.					
34973795	0	57	dep	Novel	0:4	arg1	recyclable					7:16	recyclable	7:16	recyclable	7:16	Novel, recyclable Brønsted acidic deep eutectic solvent for mild fractionation of hemicelluloses.					
34973795	5	58	theme	suitable	711:718	arg1	acidity					720:726	the suitable acidity	707:726	the suitable acidity of DESs and mild temperature	707:755	The results revealed that the suitable acidity of DESs and mild temperature effectively avoided the over-degradation of hemicelluloses.					
32356856	0	0	theme	microbiota	71:80	arg1	composition					82:92	gut microbiota composition	67:92	gut microbiota composition	67:92	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	5	1	theme	butyric	1098:1104	arg1	acid					1106:1109	butyric acid	1098:1109	butyric acid	1098:1109	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	4	2	theme	fatty	686:690	arg1	SCFA					698:701	SCFA	698:701	SCFA	698:701	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	4	2	theme	fatty	686:690	arg1	Lachnospiraceae					735:749	Lachnospiraceae	735:749	especially Lachnospiraceae	724:749	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	4	2	theme	fatty	686:690	arg1	acid					692:695	short-chain fatty acid	674:695	short-chain fatty acid (SCFA)-producing bacteria	674:721	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	0	3	theme	gut	67:69	arg1	composition					82:92	gut microbiota composition	67:92	gut microbiota composition	67:92	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	5	4	theme	acid	1106:1109	arg1	production					1055:1064	the production	1051:1064	the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice	1051:1150	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	4	5	theme	normal	764:769	arg1	mice					771:774	normal mice	764:774	normal mice	764:774	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	5	6	from	mice	1032:1035	arg1	production					987:996	the production	983:996	the production of acetic acid in the non-treated mice	983:1035	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	0	7	from	Effects	0:6	arg1	morels					37:42	wild morels	32:42	wild morels	32:42	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	0	7	from	Effects	0:6	arg1	response					54:61	immune response	47:61	immune response	47:61	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	0	7	from	Effects	0:6	arg1	composition					82:92	gut microbiota composition	67:92	gut microbiota composition	67:92	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	3	8	theme	white	530:534	arg1	cells					542:546	white blood cells	530:546	white blood cells	530:546	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	3	9	theme	lymphocytes	552:562	arg1	counts					520:525	the counts	516:525	the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice	516:620	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	5	10	theme	acid	1076:1079	arg1	production					1055:1064	the production	1051:1064	the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice	1051:1150	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	5	11	theme	valeric	956:962	arg1	acid					964:967	valeric acid	956:967	valeric acid	956:967	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	3	12	theme	blood	536:540	arg1	cells					542:546	white blood cells	530:546	white blood cells	530:546	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	5	13	from	production	987:996	arg1	mice					1032:1035	the non-treated mice	1016:1035	the non-treated mice	1016:1035	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	1	14	attach	isolated	160:167	arg1	mushrooms					174:182	mushrooms	174:182	mushrooms	174:182	Polysaccharides isolated from mushrooms have been identified as potential prebiotics that could impact gut microbiota.					
32356856	1	14	attach	isolated	160:167	arg2	Polysaccharides					144:158	Polysaccharides	144:158	Polysaccharides isolated from mushrooms	144:182	Polysaccharides isolated from mushrooms have been identified as potential prebiotics that could impact gut microbiota.					
32356856	1	14	attach	isolated	160:167	arg2	prebiotics					218:227	potential prebiotics	208:227	potential prebiotics that could impact gut microbiota	208:260	Polysaccharides isolated from mushrooms have been identified as potential prebiotics that could impact gut microbiota.					
32356856	4	15	theme	SCFA-producing	819:832	arg1	bacteria					834:841	SCFA-producing bacteria	819:841	SCFA-producing bacteria	819:841	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	5	16	theme	acid	964:967	arg1	production					942:951	the production	938:951	the production of valeric acid	938:967	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	4	17	theme	increased	654:662	arg1	levels					664:669	increased levels	654:669	increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae,	654:750	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	2	18	theme	non-treated	396:406	arg1	mice					442:445	non-treated and cyclophosphamide (CP)-treated mice	396:445	non-treated and cyclophosphamide (CP)-treated mice	396:445	In this study, a water-soluble polysaccharide (MP) extracted from wild morels was evaluated for its effects on the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice.					
32356856	0	19	theme	non-treated	97:107	arg1	mice					138:141	non-treated and cyclophosphamide-treated mice	97:141	non-treated and cyclophosphamide-treated mice	97:141	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	0	20	from	morels	37:42	arg1	polysaccharides					11:25	polysaccharides	11:25	polysaccharides from wild morels	11:42	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	0	20	from	morels	37:42	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice	0:141	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	2	21	from	effects	363:369	arg1	microbiota					382:391	the gut microbiota	374:391	the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice	374:445	In this study, a water-soluble polysaccharide (MP) extracted from wild morels was evaluated for its effects on the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice.					
32356856	2	22	theme	water-soluble	280:292	arg1	MP					310:311	MP	310:311	MP	310:311	In this study, a water-soluble polysaccharide (MP) extracted from wild morels was evaluated for its effects on the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice.					
32356856	2	22	theme	water-soluble	280:292	arg1	polysaccharide					294:307	a water-soluble polysaccharide	278:307	a water-soluble polysaccharide (MP) extracted from wild morels	278:339	In this study, a water-soluble polysaccharide (MP) extracted from wild morels was evaluated for its effects on the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice.					
32356856	5	23	theme	acid	1092:1095	arg1	production					1055:1064	the production	1051:1064	the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice	1051:1150	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	2	24	theme	wild	329:332	arg1	morels					334:339	wild morels	329:339	wild morels	329:339	In this study, a water-soluble polysaccharide (MP) extracted from wild morels was evaluated for its effects on the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice.					
32356856	3	25	dep	blood	582:586	arg1	the					567:569	the	567:569	the	567:569	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	2	26	theme	mice	442:445	arg1	microbiota					382:391	the gut microbiota	374:391	the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice	374:445	In this study, a water-soluble polysaccharide (MP) extracted from wild morels was evaluated for its effects on the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice.					
32356856	4	27	theme	bacteria	834:841	arg1	levels					791:796	increased levels	781:796	increased levels of Bacteroidetes and SCFA-producing bacteria	781:841	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	4	27	theme	bacteria	834:841	arg1	Ruminococcaceae					855:869	Ruminococcaceae	855:869	Ruminococcaceae	855:869	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	1	28	theme	potential	208:216	arg1	Polysaccharides					144:158	Polysaccharides	144:158	Polysaccharides isolated from mushrooms	144:182	Polysaccharides isolated from mushrooms have been identified as potential prebiotics that could impact gut microbiota.					
32356856	1	28	theme	potential	208:216	arg1	prebiotics					218:227	potential prebiotics	208:227	potential prebiotics that could impact gut microbiota	208:260	Polysaccharides isolated from mushrooms have been identified as potential prebiotics that could impact gut microbiota.					
32356856	2	29	theme	-treated	433:440	arg1	mice					442:445	non-treated and cyclophosphamide (CP)-treated mice	396:445	non-treated and cyclophosphamide (CP)-treated mice	396:445	In this study, a water-soluble polysaccharide (MP) extracted from wild morels was evaluated for its effects on the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice.					
32356856	5	30	theme	CP-treated	1136:1145	arg1	mice					1147:1150	the CP-treated mice	1132:1150	the CP-treated mice	1132:1150	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	5	31	theme	acetic	1069:1074	arg1	acid					1076:1079	acetic acid	1069:1079	acetic acid	1069:1079	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	3	32	theme	cells	542:546	arg1	counts					520:525	the counts	516:525	the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice	516:620	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	0	33	theme	polysaccharides	11:25	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice	0:141	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	0	34	theme	cyclophosphamide-treated	113:136	arg1	mice					138:141	non-treated and cyclophosphamide-treated mice	97:141	non-treated and cyclophosphamide-treated mice	97:141	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	4	35	theme	short-chain	674:684	arg1	SCFA					698:701	SCFA	698:701	SCFA	698:701	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	4	35	theme	short-chain	674:684	arg1	Lachnospiraceae					735:749	Lachnospiraceae	735:749	especially Lachnospiraceae	724:749	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	4	35	theme	short-chain	674:684	arg1	acid					692:695	short-chain fatty acid	674:695	short-chain fatty acid (SCFA)-producing bacteria	674:721	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	4	36	theme	acid	692:695	arg1	levels					664:669	increased levels	654:669	increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae,	654:750	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	5	37	theme	acetic	1001:1006	arg1	acid					1008:1011	acetic acid	1001:1011	acetic acid in the non-treated mice	1001:1035	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	2	38	theme	gut	378:380	arg1	microbiota					382:391	the gut microbiota	374:391	the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice	374:445	In this study, a water-soluble polysaccharide (MP) extracted from wild morels was evaluated for its effects on the gut microbiota of non-treated and cyclophosphamide (CP)-treated mice.					
32356856	0	39	theme	wild	32:35	arg1	morels					37:42	wild morels	32:42	wild morels	32:42	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	3	40	theme	mice	617:620	arg1	blood					582:586	peripheral blood	571:586	peripheral blood	571:586	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	3	40	theme	mice	617:620	arg1	spleen					592:597	spleen	592:597	spleen	592:597	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	4	41	theme	increased	781:789	arg1	levels					791:796	increased levels	781:796	increased levels of Bacteroidetes and SCFA-producing bacteria	781:841	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	4	41	theme	increased	781:789	arg1	Ruminococcaceae					855:869	Ruminococcaceae	855:869	Ruminococcaceae	855:869	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	5	42	from	production	1055:1064	arg1	mice					1147:1150	the CP-treated mice	1132:1150	the CP-treated mice	1132:1150	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	0	43	theme	immune	47:52	arg1	response					54:61	immune response	47:61	immune response	47:61	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	5	44	theme	valeric	1116:1122	arg1	acid					1124:1127	valeric acid	1116:1127	valeric acid	1116:1127	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	0	45	from	response	54:61	arg1	mice					138:141	non-treated and cyclophosphamide-treated mice	97:141	non-treated and cyclophosphamide-treated mice	97:141	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	4	46	theme	Bacteroidetes	801:813	arg1	levels					791:796	increased levels	781:796	increased levels of Bacteroidetes and SCFA-producing bacteria	781:841	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	4	46	theme	Bacteroidetes	801:813	arg1	Ruminococcaceae					855:869	Ruminococcaceae	855:869	Ruminococcaceae	855:869	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	5	47	theme	propionic	1082:1090	arg1	acid					1092:1095	propionic acid	1082:1095	propionic acid	1082:1095	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	5	48	theme	acid	1124:1127	arg1	production					1055:1064	the production	1051:1064	the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice	1051:1150	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	5	49	theme	non-treated	1020:1030	arg1	mice					1032:1035	the non-treated mice	1016:1035	the non-treated mice	1016:1035	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	3	50	theme	peripheral	571:580	arg1	blood					582:586	peripheral blood	571:586	peripheral blood	571:586	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	3	51	theme	spleen	488:493	arg1	weight					495:500	the spleen weight	484:500	the spleen weight	484:500	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	5	52	theme	acid	1008:1011	arg1	production					987:996	the production	983:996	the production of acetic acid in the non-treated mice	983:1035	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	1	53	theme	gut	247:249	arg1	microbiota					251:260	gut microbiota	247:260	gut microbiota	247:260	Polysaccharides isolated from mushrooms have been identified as potential prebiotics that could impact gut microbiota.					
32356856	4	54	theme	CP-treated	888:897	arg1	mice					899:902	the CP-treated mice	884:902	the CP-treated mice	884:902	Mice treated with MP exhibited increased levels of short-chain fatty acid (SCFA)-producing bacteria, especially Lachnospiraceae, compared to normal mice, and increased levels of Bacteroidetes and SCFA-producing bacteria, especially Ruminococcaceae, compared to the CP-treated mice.					
32356856	3	55	theme	CP-treated	606:615	arg1	mice					617:620	the CP-treated mice	602:620	the CP-treated mice	602:620	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	5	56	theme	MP	915:916	arg1	treatment					918:926	MP treatment	915:926	MP treatment	915:926	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	5	57	from	acid	1008:1011	arg1	mice					1032:1035	the non-treated mice	1016:1035	the non-treated mice	1016:1035	Moreover, MP treatment increased the production of valeric acid and decreased the production of acetic acid in the non-treated mice and increased the production of acetic acid, propionic acid, butyric acid, and valeric acid in the CP-treated mice.					
32356856	0	58	from	composition	82:92	arg1	mice					138:141	non-treated and cyclophosphamide-treated mice	97:141	non-treated and cyclophosphamide-treated mice	97:141	Effects of polysaccharides from wild morels on immune response and gut microbiota composition in non-treated and cyclophosphamide-treated mice.					
32356856	3	59	from	counts	520:525	arg1	blood					582:586	peripheral blood	571:586	peripheral blood	571:586	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
32356856	3	59	from	counts	520:525	arg1	spleen					592:597	spleen	592:597	spleen	592:597	The results showed that MP restored the spleen weight and increased the counts of white blood cells and lymphocytes in the peripheral blood and spleen of the CP-treated mice.					
34481850	3	0	theme	TiO2-N	696:701	arg1	peak					646:649	no novel absorption peak	626:649	no novel absorption peak in FTIR spectra	626:665	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	0	theme	TiO2-N	696:701	arg1	peaks					687:691	characteristic peaks	672:691	characteristic peaks of TiO2-N	672:701	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	2	1	from	properties	451:460	arg1	combination					364:374	the moisture combination	351:374	the moisture combination	351:374	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	6	2	theme	network	1041:1047	arg1	structures					1049:1058	stable and compact network structures	1022:1058	stable and compact network structures	1022:1058	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	5	3	theme	invisible	992:1000	arg1	light					1002:1006	invisible light	992:1006	invisible light	992:1006	TiO2-N and MCP positively affected anti-UV light ability of HBS films by resisting most of invisible light.					
34481850	1	4	theme	oxide	254:258	arg1	agent					232:236	the reinforcement agent	214:236	the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP)	214:315	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	2	5	theme	films	465:469	arg1	effects					322:328	The effects	318:328	The effects of TiO2-N and MCP on the moisture combination	318:374	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	2	5	theme	films	465:469	arg1	properties					389:398	rheological properties	377:398	rheological properties of film-forming solutions (FFS)	377:430	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	2	5	theme	films	465:469	arg1	properties					451:460	physiochemical properties	436:460	physiochemical properties of films	436:469	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	2	6	theme	TiO2-N	333:338	arg1	effects					322:328	The effects	318:328	The effects of TiO2-N and MCP on the moisture combination	318:374	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	2	6	theme	TiO2-N	333:338	arg1	properties					389:398	rheological properties	377:398	rheological properties of film-forming solutions (FFS)	377:430	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	2	6	theme	TiO2-N	333:338	arg1	properties					451:460	physiochemical properties	436:460	physiochemical properties of films	436:469	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	6	7	dep	%	1170:1170	arg1	to					1172:1173	to	1172:1173	to	1172:1173	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	0	8	theme	Mesona	103:108	arg1	polysaccharide					126:139	Mesona chinensis Benth polysaccharide	103:139	Mesona chinensis Benth polysaccharide	103:139	Preparation and characterization of hyacinth bean starch film incorporated with TiO2 nanoparticles and Mesona chinensis Benth polysaccharide.					
34481850	7	9	theme	metallic	1420:1427	arg1	oxide					1429:1433	metallic oxide	1420:1433	metallic oxide	1420:1433	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	6	10	theme	heat	1202:1205	arg1	resistance					1207:1216	heat resistance	1202:1216	heat resistance	1202:1216	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	3	11	theme	infrared	508:515	arg1	diffraction					544:554	X-ray diffraction	538:554	X-ray diffraction (XRD)	538:560	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	11	theme	infrared	508:515	arg1	FTIR					531:534	FTIR	531:534	FTIR	531:534	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	11	theme	infrared	508:515	arg1	spectroscopy					517:528	infrared spectroscopy	508:528	infrared spectroscopy (FTIR)	508:535	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	7	12	theme	functional	1364:1373	arg1	films					1375:1379	functional films	1364:1379	functional films	1364:1379	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	7	13	theme	guiding	1324:1330	arg1	significance					1332:1343	guiding significance	1324:1343	guiding significance	1324:1343	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	4	14	theme	films	894:898	arg1	hydrophobicity					876:889	hydrophobicity	876:889	hydrophobicity of films	876:898	Contact angle of HBS/TiO2-N/M3 film increased from 65.6° to 90.9°, which illustrated that TiO2-N and MCP effectively enhanced hydrophobicity of films.					
34481850	3	15	theme	characteristic	672:685	arg1	peaks					687:691	characteristic peaks	672:691	characteristic peaks of TiO2-N	672:701	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	7	16	theme	films	1375:1379	arg1	development					1349:1359	development	1349:1359	development of functional films and combination of polysaccharides and metallic oxide	1349:1433	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	3	17	from	peaks	687:691	arg1	spectra					659:665	FTIR spectra	654:665	FTIR spectra	654:665	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	0	18	theme	Benth	120:124	arg1	polysaccharide					126:139	Mesona chinensis Benth polysaccharide	103:139	Mesona chinensis Benth polysaccharide	103:139	Preparation and characterization of hyacinth bean starch film incorporated with TiO2 nanoparticles and Mesona chinensis Benth polysaccharide.					
34481850	7	19	theme	polysaccharides	1400:1414	arg1	combination					1385:1395	combination	1385:1395	combination of polysaccharides and metallic oxide	1385:1433	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	7	19	theme	polysaccharides	1400:1414	arg1	films					1375:1379	functional films	1364:1379	functional films	1364:1379	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	2	20	from	effects	322:328	arg1	combination					364:374	the moisture combination	351:374	the moisture combination	351:374	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	7	21	theme	HBS-based	1276:1284	arg1	films					1286:1290	a nanocomposite HBS-based films	1260:1290	a nanocomposite HBS-based films	1260:1290	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	0	22	theme	chinensis	110:118	arg1	polysaccharide					126:139	Mesona chinensis Benth polysaccharide	103:139	Mesona chinensis Benth polysaccharide	103:139	Preparation and characterization of hyacinth bean starch film incorporated with TiO2 nanoparticles and Mesona chinensis Benth polysaccharide.					
34481850	4	23	theme	Contact	750:756	arg1	angle					758:762	Contact angle	750:762	Contact angle of HBS/TiO2-N/M3 film	750:784	Contact angle of HBS/TiO2-N/M3 film increased from 65.6° to 90.9°, which illustrated that TiO2-N and MCP effectively enhanced hydrophobicity of films.					
34481850	5	24	theme	anti-UV	936:942	arg1	ability					950:956	anti-UV light ability	936:956	anti-UV light ability of HBS films	936:969	TiO2-N and MCP positively affected anti-UV light ability of HBS films by resisting most of invisible light.					
34481850	5	25	theme	HBS	961:963	arg1	films					965:969	HBS films	961:969	HBS films	961:969	TiO2-N and MCP positively affected anti-UV light ability of HBS films by resisting most of invisible light.					
34481850	6	26	theme	stable	1022:1027	arg1	structures					1049:1058	stable and compact network structures	1022:1058	stable and compact network structures	1022:1058	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	0	27	theme	TiO2	80:83	arg1	nanoparticles					85:97	TiO2 nanoparticles	80:97	TiO2 nanoparticles	80:97	Preparation and characterization of hyacinth bean starch film incorporated with TiO2 nanoparticles and Mesona chinensis Benth polysaccharide.					
34481850	3	28	dep	Fourier	490:496	arg1	transform					498:506	transform	498:506	transform infrared spectroscopy (FTIR), X-ray diffraction (XRD)	498:560	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	2	29	theme	moisture	355:362	arg1	combination					364:374	the moisture combination	351:374	the moisture combination	351:374	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	6	30	theme	28.603	1175:1180	arg1	%					1170:1170	%	1170:1170	%	1170:1170	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	6	31	theme	TiO2-N	1101:1106	arg1	effect					1091:1096	the synergistic effect	1075:1096	the synergistic effect of TiO2-N and MCP	1075:1114	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	1	32	theme	Mesona	273:278	arg1	MCP					312:314	MCP	312:314	MCP	312:314	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	1	32	theme	Mesona	273:278	arg1	polysaccharide					296:309	Mesona chinensis Benth polysaccharide	273:309	Mesona chinensis Benth polysaccharide (MCP)	273:315	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	3	33	theme	X-ray	538:542	arg1	diffraction					544:554	X-ray diffraction	538:554	X-ray diffraction (XRD)	538:560	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	33	theme	X-ray	538:542	arg1	spectroscopy					517:528	infrared spectroscopy	508:528	infrared spectroscopy (FTIR)	508:535	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	33	theme	X-ray	538:542	arg1	XRD					557:559	XRD	557:559	XRD	557:559	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	5	34	theme	films	965:969	arg1	ability					950:956	anti-UV light ability	936:956	anti-UV light ability of HBS films	936:969	TiO2-N and MCP positively affected anti-UV light ability of HBS films by resisting most of invisible light.					
34481850	1	35	theme	nanocomposite	189:201	arg1	films					203:207	nanocomposite films	189:207	nanocomposite films	189:207	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	2	36	theme	solutions	416:424	arg1	effects					322:328	The effects	318:328	The effects of TiO2-N and MCP on the moisture combination	318:374	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	2	36	theme	solutions	416:424	arg1	properties					389:398	rheological properties	377:398	rheological properties of film-forming solutions (FFS)	377:430	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	2	36	theme	solutions	416:424	arg1	properties					451:460	physiochemical properties	436:460	physiochemical properties of films	436:469	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	1	37	theme	chinensis	280:288	arg1	MCP					312:314	MCP	312:314	MCP	312:314	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	1	37	theme	chinensis	280:288	arg1	polysaccharide					296:309	Mesona chinensis Benth polysaccharide	273:309	Mesona chinensis Benth polysaccharide (MCP)	273:315	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	6	38	theme	compact	1033:1039	arg1	structures					1049:1058	stable and compact network structures	1022:1058	stable and compact network structures	1022:1058	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	3	39	contain	had	596:598	arg1	TiO2-N					581:586	TiO2-N	581:586	TiO2-N	581:586	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	39	contain	had	596:598	arg1	HBS					576:578	HBS	576:578	HBS	576:578	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	39	contain	had	596:598	arg1	MCP					592:594	MCP	592:594	MCP	592:594	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	39	contain	had	596:598	arg2	compatibility					605:617	good compatibility	600:617	good compatibility	600:617	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	40	from	peak	646:649	arg1	spectra					659:665	FTIR spectra	654:665	FTIR spectra	654:665	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	1	41	theme	Benth	290:294	arg1	MCP					312:314	MCP	312:314	MCP	312:314	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	1	41	theme	Benth	290:294	arg1	polysaccharide					296:309	Mesona chinensis Benth polysaccharide	273:309	Mesona chinensis Benth polysaccharide (MCP)	273:315	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	6	42	theme	17.123	1164:1169	arg1	%					1170:1170	%	1170:1170	%	1170:1170	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	4	43	dep	90.9°	810:814	arg1	to					807:808	to	807:808	to	807:808	Contact angle of HBS/TiO2-N/M3 film increased from 65.6° to 90.9°, which illustrated that TiO2-N and MCP effectively enhanced hydrophobicity of films.					
34481850	1	44	theme	polysaccharide	296:309	arg1	agent					232:236	the reinforcement agent	214:236	the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP)	214:315	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	7	45	theme	combination	1385:1395	arg1	development					1349:1359	development	1349:1359	development of functional films and combination of polysaccharides and metallic oxide	1349:1433	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	2	46	theme	rheological	377:387	arg1	properties					389:398	rheological properties	377:398	rheological properties of film-forming solutions (FFS)	377:430	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	0	47	theme	film	57:60	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of hyacinth bean starch film incorporated with TiO2 nanoparticles and Mesona chinensis Benth polysaccharide.					
34481850	0	47	theme	film	57:60	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of hyacinth bean starch film incorporated with TiO2 nanoparticles and Mesona chinensis Benth polysaccharide.					
34481850	3	48	located	found	708:712	arg2	peaks					687:691	characteristic peaks	672:691	characteristic peaks of TiO2-N	672:701	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	48	located	found	708:712	arg2	peak					646:649	no novel absorption peak	626:649	no novel absorption peak in FTIR spectra	626:665	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	48	located	found	708:712	arg1	patterns					721:728	XRD patterns	717:728	XRD patterns of composite films	717:747	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	49	theme	good	600:603	arg1	compatibility					605:617	good compatibility	600:617	good compatibility	600:617	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	4	50	theme	film	781:784	arg1	angle					758:762	Contact angle	750:762	Contact angle of HBS/TiO2-N/M3 film	750:784	Contact angle of HBS/TiO2-N/M3 film increased from 65.6° to 90.9°, which illustrated that TiO2-N and MCP effectively enhanced hydrophobicity of films.					
34481850	6	51	theme	synergistic	1079:1089	arg1	effect					1091:1096	the synergistic effect	1075:1096	the synergistic effect of TiO2-N and MCP	1075:1114	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	0	52	theme	starch	50:55	arg1	film					57:60	hyacinth bean starch film	36:60	hyacinth bean starch film	36:60	Preparation and characterization of hyacinth bean starch film incorporated with TiO2 nanoparticles and Mesona chinensis Benth polysaccharide.					
34481850	7	53	theme	oxide	1429:1433	arg1	combination					1385:1395	combination	1385:1395	combination of polysaccharides and metallic oxide	1385:1433	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	7	53	theme	oxide	1429:1433	arg1	films					1375:1379	functional films	1364:1379	functional films	1364:1379	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	3	54	theme	novel	629:633	arg1	peak					646:649	no novel absorption peak	626:649	no novel absorption peak in FTIR spectra	626:665	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	4	55	theme	HBS/TiO2-N/M3	767:779	arg1	film					781:784	HBS/TiO2-N/M3 film	767:784	HBS/TiO2-N/M3 film	767:784	Contact angle of HBS/TiO2-N/M3 film increased from 65.6° to 90.9°, which illustrated that TiO2-N and MCP effectively enhanced hydrophobicity of films.					
34481850	7	56	contain	had	1320:1322	arg1	films					1286:1290	a nanocomposite HBS-based films	1260:1290	a nanocomposite HBS-based films	1260:1290	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	7	56	contain	had	1320:1322	arg2	significance					1332:1343	guiding significance	1324:1343	guiding significance	1324:1343	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	3	57	theme	XRD	717:719	arg1	patterns					721:728	XRD patterns	717:728	XRD patterns of composite films	717:747	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	58	theme	absorption	635:644	arg1	peak					646:649	no novel absorption peak	626:649	no novel absorption peak in FTIR spectra	626:665	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	5	59	theme	light	944:948	arg1	ability					950:956	anti-UV light ability	936:956	anti-UV light ability of HBS films	936:969	TiO2-N and MCP positively affected anti-UV light ability of HBS films by resisting most of invisible light.					
34481850	1	60	theme	reinforcement	218:230	arg1	agent					232:236	the reinforcement agent	214:236	the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP)	214:315	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	6	61	theme	MCP	1112:1114	arg1	effect					1091:1096	the synergistic effect	1075:1096	the synergistic effect of TiO2-N and MCP	1075:1114	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	6	62	dep	formed	1065:1070	arg1	enhanced					1222:1229	enhanced	1222:1229	was enhanced clearly	1218:1237	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	6	62	dep	formed	1065:1070	arg1	increased					1149:1157	increased	1149:1157	was increased from 17.123% to 28.603% significantly	1145:1195	Furthermore, stable and compact network structures were formed by the synergistic effect of TiO2-N and MCP, thereby elongation to break was increased from 17.123% to 28.603% significantly, and heat resistance was enhanced clearly.					
34481850	2	63	from	properties	389:398	arg1	combination					364:374	the moisture combination	351:374	the moisture combination	351:374	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	1	64	used	used	173:176	arg2	HBS					164:166	HBS	164:166	HBS	164:166	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	1	64	used	used	173:176	arg2	bean					151:154	Hyacinth bean starch	142:161	Hyacinth bean starch (HBS)	142:167	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	2	65	theme	film-forming	403:414	arg1	FFS					427:429	FFS	427:429	FFS	427:429	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	2	65	theme	film-forming	403:414	arg1	solutions					416:424	film-forming solutions	403:424	film-forming solutions (FFS)	403:430	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	1	66	theme	starch	156:161	arg1	bean					151:154	Hyacinth bean starch	142:161	Hyacinth bean starch (HBS)	142:167	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	1	66	theme	starch	156:161	arg1	HBS					164:166	HBS	164:166	HBS	164:166	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	7	67	theme	nanocomposite	1262:1274	arg1	films					1286:1290	a nanocomposite HBS-based films	1260:1290	a nanocomposite HBS-based films	1260:1290	This study prepared a nanocomposite HBS-based films based TiO2-N and MCP, which had guiding significance for development of functional films and combination of polysaccharides and metallic oxide.					
34481850	3	68	theme	composite	733:741	arg1	films					743:747	composite films	733:747	composite films	733:747	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	2	69	theme	physiochemical	436:449	arg1	properties					451:460	physiochemical properties	436:460	physiochemical properties of films	436:469	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	3	70	theme	FTIR	654:657	arg1	spectra					659:665	FTIR spectra	654:665	FTIR spectra	654:665	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	3	71	theme	films	743:747	arg1	patterns					721:728	XRD patterns	717:728	XRD patterns of composite films	717:747	Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) revealed that HBS, TiO2-N and MCP had good compatibility, while no novel absorption peak in FTIR spectra, and characteristic peaks of TiO2-N were found in XRD patterns of composite films.					
34481850	2	72	theme	MCP	344:346	arg1	effects					322:328	The effects	318:328	The effects of TiO2-N and MCP on the moisture combination	318:374	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	2	72	theme	MCP	344:346	arg1	properties					389:398	rheological properties	377:398	rheological properties of film-forming solutions (FFS)	377:430	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	2	72	theme	MCP	344:346	arg1	properties					451:460	physiochemical properties	436:460	physiochemical properties of films	436:469	The effects of TiO2-N and MCP on the moisture combination, rheological properties of film-forming solutions (FFS) and physiochemical properties of films were investigated.					
34481850	1	73	theme	nanotitanium	241:252	arg1	TiO2-N					261:266	TiO2-N	261:266	TiO2-N	261:266	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34481850	1	73	theme	nanotitanium	241:252	arg1	oxide					254:258	nanotitanium oxide	241:258	nanotitanium oxide (TiO2-N)	241:267	Hyacinth bean starch (HBS) was used to prepare nanocomposite films with the reinforcement agent of nanotitanium oxide (TiO2-N) and Mesona chinensis Benth polysaccharide (MCP).					
34560153	8	0	theme	network	1512:1518	arg1	structure					1520:1528	strong and ordered gel network structure	1489:1528	strong and ordered gel network structure	1489:1528	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	6	1	theme	KBST	1097:1100	arg1	strength					1032:1039	gel strength	1028:1039	gel strength	1028:1039	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	1	theme	KBST	1097:1100	arg1	viscosity					964:972	The pasting viscosity	952:972	The pasting viscosity	952:972	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	1	theme	KBST	1097:1100	arg1	viscoelasticity					1011:1025	viscoelasticity	1011:1025	viscoelasticity	1011:1025	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	1	theme	KBST	1097:1100	arg1	stability					1055:1063	cold storage stability	1042:1063	cold storage stability	1042:1063	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	1	theme	KBST	1097:1100	arg1	degree					984:989	swelling degree	975:989	swelling degree of starch granules	975:1008	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	1	theme	KBST	1097:1100	arg1	capacity					1085:1092	water-retention capacity	1069:1092	water-retention capacity	1069:1092	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	2	theme	granules	1001:1008	arg1	strength					1032:1039	gel strength	1028:1039	gel strength	1028:1039	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	2	theme	granules	1001:1008	arg1	viscosity					964:972	The pasting viscosity	952:972	The pasting viscosity	952:972	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	2	theme	granules	1001:1008	arg1	viscoelasticity					1011:1025	viscoelasticity	1011:1025	viscoelasticity	1011:1025	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	2	theme	granules	1001:1008	arg1	stability					1055:1063	cold storage stability	1042:1063	cold storage stability	1042:1063	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	2	theme	granules	1001:1008	arg1	degree					984:989	swelling degree	975:989	swelling degree of starch granules	975:1008	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	2	theme	granules	1001:1008	arg1	capacity					1085:1092	water-retention capacity	1069:1092	water-retention capacity	1069:1092	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	8	3	theme	digestible	1346:1355	arg1	starch					1357:1362	slowly digestible starch	1339:1362	slowly digestible starch	1339:1362	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	8	4	theme	storage	1407:1413	arg1	modulus					1415:1421	the storage modulus	1403:1421	the storage modulus	1403:1421	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	1	5	theme	Auricularia	163:173	arg1	AP					206:207	AP	206:207	AP	206:207	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	1	5	theme	Auricularia	163:173	arg1	polysaccharide					190:203	Auricularia auricula-judae polysaccharide	163:203	Auricularia auricula-judae polysaccharide (AP)	163:208	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	8	6	theme	strong	1489:1494	arg1	structure					1520:1528	strong and ordered gel network structure	1489:1528	strong and ordered gel network structure	1489:1528	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	1	7	theme	excellent	274:282	arg1	property					296:303	excellent gel-forming property	274:303	excellent gel-forming property	274:303	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	0	8	from	Effects	0:6	arg1	rheology					81:88	rheology	81:88	rheology	81:88	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	0	8	from	Effects	0:6	arg1	properties					102:111	structural properties	91:111	structural properties	91:111	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	0	8	from	Effects	0:6	arg1	gelatinization					65:78	gelatinization	65:78	gelatinization	65:78	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	0	8	from	Effects	0:6	arg1	pasting					56:62	pasting	56:62	pasting	56:62	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	0	8	from	Effects	0:6	arg1	digestibility					126:138	in vitro digestibility	117:138	in vitro digestibility	117:138	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	8	9	theme	reduced	1602:1608	arg1	digestibility					1617:1629	the reduced starch digestibility	1598:1629	the reduced starch digestibility	1598:1629	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	0	10	dep	in	117:118	arg1	vitro					120:124	vitro	120:124	vitro	120:124	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	1	11	theme	gel-forming	284:294	arg1	property					296:303	excellent gel-forming property	274:303	excellent gel-forming property	274:303	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	0	12	theme	structural	91:100	arg1	properties					102:111	structural properties	91:111	structural properties	91:111	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	8	13	theme	gel	1508:1510	arg1	structure					1520:1528	strong and ordered gel network structure	1489:1528	strong and ordered gel network structure	1489:1528	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	4	14	theme	low-field	738:746	arg1	analyzer					674:681	rapid visco analyzer	662:681	rapid visco analyzer	662:681	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	14	theme	low-field	738:746	arg1	resonance					765:773	low-field nuclear magnetic resonance	738:773	low-field nuclear magnetic resonance	738:773	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	3	15	theme	starch	543:548	arg1	microstructure					485:498	microstructure	485:498	microstructure	485:498	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	3	15	theme	starch	543:548	arg1	rheology					475:482	rheology	475:482	rheology	475:482	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	3	15	theme	starch	543:548	arg1	gelatinization					459:472	gelatinization	459:472	gelatinization	459:472	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	3	15	theme	starch	543:548	arg1	pasting					450:456	pasting	450:456	pasting	450:456	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	3	15	theme	starch	543:548	arg1	digestibility					514:526	in vitro digestibility	505:526	in vitro digestibility	505:526	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	8	16	theme	resistant	1318:1326	arg1	starch					1328:1333	resistant starch	1318:1333	resistant starch	1318:1333	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	0	17	theme	in	117:118	arg1	digestibility					126:138	in vitro digestibility	117:138	in vitro digestibility	117:138	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	4	18	theme	laser	713:717	arg1	analyzer					728:735	laser particle analyzer	713:735	laser particle analyzer	713:735	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	18	theme	laser	713:717	arg1	analyzer					674:681	rapid visco analyzer	662:681	rapid visco analyzer	662:681	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	6	19	theme	cold	1042:1045	arg1	viscosity					964:972	The pasting viscosity	952:972	The pasting viscosity	952:972	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	19	theme	cold	1042:1045	arg1	stability					1055:1063	cold storage stability	1042:1063	cold storage stability	1042:1063	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	20	theme	gel	1028:1030	arg1	viscosity					964:972	The pasting viscosity	952:972	The pasting viscosity	952:972	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	20	theme	gel	1028:1030	arg1	strength					1032:1039	gel strength	1028:1039	gel strength	1028:1039	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	4	21	dep	Fourier	776:782	arg1	transform					784:792	transform	784:792	transform infrared spectroscopy and scanning electron microscopy, respectively	784:861	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	1	22	theme	auricula-judae	175:188	arg1	AP					206:207	AP	206:207	AP	206:207	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	1	22	theme	auricula-judae	175:188	arg1	polysaccharide					190:203	Auricularia auricula-judae polysaccharide	163:203	Auricularia auricula-judae polysaccharide (AP)	163:208	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	8	23	theme	structure	1520:1528	arg1	formation					1476:1484	the formation	1472:1484	the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST	1472:1576	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	8	23	theme	structure	1520:1528	arg1	responsible					1582:1592	responsible	1582:1592	responsible	1582:1592	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	3	24	theme	kidney	531:536	arg1	KBST					551:554	KBST	551:554	KBST	551:554	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	3	24	theme	kidney	531:536	arg1	starch					543:548	kidney bean starch	531:548	kidney bean starch (KBST)	531:555	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	4	25	dep	transform	784:792	arg1	infrared					794:801	infrared	794:801	transform infrared spectroscopy and scanning electron microscopy, respectively	784:861	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	7	26	theme	KBST	1176:1179	arg1	combination					1154:1164	The combination	1150:1164	The combination of AP and KBST	1150:1179	The combination of AP and KBST exhibited a higher short-range ordered and a firmer and denser structure than that of KBST alone.					
34560153	6	27	theme	pasting	956:962	arg1	strength					1032:1039	gel strength	1028:1039	gel strength	1028:1039	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	27	theme	pasting	956:962	arg1	viscosity					964:972	The pasting viscosity	952:972	The pasting viscosity	952:972	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	27	theme	pasting	956:962	arg1	viscoelasticity					1011:1025	viscoelasticity	1011:1025	viscoelasticity	1011:1025	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	27	theme	pasting	956:962	arg1	stability					1055:1063	cold storage stability	1042:1063	cold storage stability	1042:1063	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	27	theme	pasting	956:962	arg1	degree					984:989	swelling degree	975:989	swelling degree of starch granules	975:1008	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	27	theme	pasting	956:962	arg1	capacity					1085:1092	water-retention capacity	1069:1092	water-retention capacity	1069:1092	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	8	28	theme	synergistic	1533:1543	arg1	interactions					1545:1556	synergistic interactions	1533:1556	synergistic interactions between AP and KBST	1533:1576	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	4	29	theme	particle	719:726	arg1	analyzer					728:735	laser particle analyzer	713:735	laser particle analyzer	713:735	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	29	theme	particle	719:726	arg1	analyzer					674:681	rapid visco analyzer	662:681	rapid visco analyzer	662:681	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	8	30	theme	ordered	1500:1506	arg1	structure					1520:1528	strong and ordered gel network structure	1489:1528	strong and ordered gel network structure	1489:1528	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	4	31	theme	rapid	662:666	arg1	analyzer					728:735	laser particle analyzer	713:735	laser particle analyzer	713:735	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	31	theme	rapid	662:666	arg1	rheometry					684:692	rheometry	684:692	rheometry	684:692	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	31	theme	rapid	662:666	arg1	analyzer					703:710	texture analyzer	695:710	texture analyzer	695:710	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	31	theme	rapid	662:666	arg1	analyzer					674:681	rapid visco analyzer	662:681	rapid visco analyzer	662:681	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	31	theme	rapid	662:666	arg1	resonance					765:773	low-field nuclear magnetic resonance	738:773	low-field nuclear magnetic resonance	738:773	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	6	32	theme	swelling	975:982	arg1	viscosity					964:972	The pasting viscosity	952:972	The pasting viscosity	952:972	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	32	theme	swelling	975:982	arg1	degree					984:989	swelling degree	975:989	swelling degree of starch granules	975:1008	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	2	33	theme	few	337:339	arg1	researches					341:350	few researches	337:350	few researches	337:350	However, few researches focus on the interactions between AP and legume starches.					
34560153	4	34	theme	nuclear	748:754	arg1	analyzer					674:681	rapid visco analyzer	662:681	rapid visco analyzer	662:681	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	34	theme	nuclear	748:754	arg1	resonance					765:773	low-field nuclear magnetic resonance	738:773	low-field nuclear magnetic resonance	738:773	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	3	35	theme	AP	440:441	arg1	effects					429:435	the effects	425:435	the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST)	425:555	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	0	36	theme	auricula-judae	23:36	arg1	polysaccharide					38:51	Auricularia auricula-judae polysaccharide	11:51	Auricularia auricula-judae polysaccharide	11:51	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	1	37	theme	thermal	309:315	arg1	tolerance					317:325	thermal tolerance	309:325	thermal tolerance	309:325	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	6	38	theme	storage	1047:1053	arg1	viscosity					964:972	The pasting viscosity	952:972	The pasting viscosity	952:972	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	38	theme	storage	1047:1053	arg1	stability					1055:1063	cold storage stability	1042:1063	cold storage stability	1042:1063	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	0	39	theme	Auricularia	11:21	arg1	polysaccharide					38:51	Auricularia auricula-judae polysaccharide	11:51	Auricularia auricula-judae polysaccharide	11:51	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	5	40	theme	composites	940:949	arg1	digestibility					915:927	the digestibility	911:927	the digestibility of AP-KBST composites	911:949	And an in vitro method was employed to measure the digestibility of AP-KBST composites.					
34560153	3	41	theme	in	505:506	arg1	digestibility					514:526	in vitro digestibility	505:526	in vitro digestibility	505:526	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	4	42	theme	analyzer	674:681	arg1	Fourier					776:782	rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier	662:782	rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively	662:861	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	6	43	theme	AP	1132:1133	arg1	concentration					1135:1147	AP concentration	1132:1147	AP concentration	1132:1147	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	0	44	theme	kidney	143:148	arg1	starch					155:160	kidney bean starch	143:160	kidney bean starch	143:160	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	4	45	theme	mixtures	631:638	arg1	properties					609:618	The pasting, gelling and structural properties	573:618	The pasting, gelling and structural properties of AP-KBST mixtures	573:638	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	5	46	dep	in	871:872	arg1	vitro					874:878	vitro	874:878	vitro	874:878	And an in vitro method was employed to measure the digestibility of AP-KBST composites.					
34560153	1	47	theme	unique	214:219	arg1	structures					231:240	unique molecular structures	214:240	unique molecular structures	214:240	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	0	48	theme	polysaccharide	38:51	arg1	Effects					0:6	Effects	0:6	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.	0:161	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	5	49	theme	in	871:872	arg1	method					880:885	an in vitro method	868:885	an in vitro method	868:885	And an in vitro method was employed to measure the digestibility of AP-KBST composites.					
34560153	6	50	theme	starch	994:999	arg1	granules					1001:1008	starch granules	994:1008	starch granules	994:1008	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	4	51	theme	structural	598:607	arg1	properties					609:618	The pasting, gelling and structural properties	573:618	The pasting, gelling and structural properties of AP-KBST mixtures	573:638	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	1	52	theme	molecular	221:229	arg1	structures					231:240	unique molecular structures	214:240	unique molecular structures	214:240	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	0	53	theme	starch	155:160	arg1	rheology					81:88	rheology	81:88	rheology	81:88	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	0	53	theme	starch	155:160	arg1	properties					102:111	structural properties	91:111	structural properties	91:111	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	0	53	theme	starch	155:160	arg1	gelatinization					65:78	gelatinization	65:78	gelatinization	65:78	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	0	53	theme	starch	155:160	arg1	pasting					56:62	pasting	56:62	pasting	56:62	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	0	53	theme	starch	155:160	arg1	digestibility					126:138	in vitro digestibility	117:138	in vitro digestibility	117:138	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	8	54	theme	order	1441:1445	arg1	contents					1306:1313	the contents	1302:1313	the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus	1302:1421	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	8	54	theme	order	1441:1445	arg1	degree					1431:1436	the degree	1427:1436	the degree of order	1427:1445	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	4	55	theme	electron	829:836	arg1	microscopy					838:847	scanning electron microscopy	820:847	scanning electron microscopy	820:847	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	8	56	theme	starch	1357:1362	arg1	contents					1306:1313	the contents	1302:1313	the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus	1302:1421	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	8	56	theme	starch	1357:1362	arg1	degree					1431:1436	the degree	1427:1436	the degree of order	1427:1445	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	4	57	theme	texture	695:701	arg1	analyzer					703:710	texture analyzer	695:710	texture analyzer	695:710	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	57	theme	texture	695:701	arg1	analyzer					674:681	rapid visco analyzer	662:681	rapid visco analyzer	662:681	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	3	58	from	effects	429:435	arg1	microstructure					485:498	microstructure	485:498	microstructure	485:498	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	3	58	from	effects	429:435	arg1	rheology					475:482	rheology	475:482	rheology	475:482	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	3	58	from	effects	429:435	arg1	gelatinization					459:472	gelatinization	459:472	gelatinization	459:472	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	3	58	from	effects	429:435	arg1	pasting					450:456	pasting	450:456	pasting	450:456	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	3	58	from	effects	429:435	arg1	digestibility					514:526	in vitro digestibility	505:526	in vitro digestibility	505:526	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	0	59	theme	bean	150:153	arg1	starch					155:160	kidney bean starch	143:160	kidney bean starch	143:160	Effects of Auricularia auricula-judae polysaccharide on pasting, gelatinization, rheology, structural properties and in vitro digestibility of kidney bean starch.					
34560153	4	60	theme	scanning	820:827	arg1	microscopy					838:847	scanning electron microscopy	820:847	scanning electron microscopy	820:847	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	3	61	dep	in	505:506	arg1	vitro					508:512	vitro	508:512	vitro	508:512	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	8	62	theme	starch	1610:1615	arg1	digestibility					1617:1629	the reduced starch digestibility	1598:1629	the reduced starch digestibility	1598:1629	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	7	63	theme	AP	1169:1170	arg1	combination					1154:1164	The combination	1150:1164	The combination of AP and KBST	1150:1179	The combination of AP and KBST exhibited a higher short-range ordered and a firmer and denser structure than that of KBST alone.					
34560153	5	64	theme	AP-KBST	932:938	arg1	composites					940:949	AP-KBST composites	932:949	AP-KBST composites	932:949	And an in vitro method was employed to measure the digestibility of AP-KBST composites.					
34560153	3	65	dep	pasting	450:456	arg1	the					446:448	the	446:448	the	446:448	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	4	66	theme	gelling	586:592	arg1	properties					609:618	The pasting, gelling and structural properties	573:618	The pasting, gelling and structural properties of AP-KBST mixtures	573:638	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	67	theme	magnetic	756:763	arg1	analyzer					674:681	rapid visco analyzer	662:681	rapid visco analyzer	662:681	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	67	theme	magnetic	756:763	arg1	resonance					765:773	low-field nuclear magnetic resonance	738:773	low-field nuclear magnetic resonance	738:773	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	1	68	contain	has	210:212	arg2	structures					231:240	unique molecular structures	214:240	unique molecular structures	214:240	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	1	68	contain	has	210:212	arg1	AP					206:207	AP	206:207	AP	206:207	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	1	68	contain	has	210:212	arg2	bioactivities					255:267	multiple bioactivities	246:267	multiple bioactivities	246:267	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	1	68	contain	has	210:212	arg1	polysaccharide					190:203	Auricularia auricula-judae polysaccharide	163:203	Auricularia auricula-judae polysaccharide (AP)	163:208	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	3	69	theme	bean	538:541	arg1	KBST					551:554	KBST	551:554	KBST	551:554	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	3	69	theme	bean	538:541	arg1	starch					543:548	kidney bean starch	531:548	kidney bean starch (KBST)	531:555	In this study, the effects of AP on the pasting, gelatinization, rheology, microstructure, and in vitro digestibility of kidney bean starch (KBST) were evaluated.					
34560153	8	70	theme	starch	1328:1333	arg1	contents					1306:1313	the contents	1302:1313	the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus	1302:1421	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	8	70	theme	starch	1328:1333	arg1	degree					1431:1436	the degree	1427:1436	the degree of order	1427:1445	Moreover, AP increased the contents of resistant starch and slowly digestible starch, which were positively correlated with the storage modulus and the degree of order, thereby suggesting that the formation of strong and ordered gel network structure by synergistic interactions between AP and KBST was responsible for the reduced starch digestibility.					
34560153	7	71	theme	firmer	1226:1231	arg1	structure					1244:1252	and a firmer and denser structure	1220:1252	structure	1244:1252	The combination of AP and KBST exhibited a higher short-range ordered and a firmer and denser structure than that of KBST alone.					
34560153	4	72	theme	AP-KBST	623:629	arg1	mixtures					631:638	AP-KBST mixtures	623:638	AP-KBST mixtures	623:638	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	2	73	theme	legume	393:398	arg1	starches					400:407	legume starches	393:407	legume starches	393:407	However, few researches focus on the interactions between AP and legume starches.					
34560153	6	74	theme	water-retention	1069:1083	arg1	viscosity					964:972	The pasting viscosity	952:972	The pasting viscosity	952:972	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	6	74	theme	water-retention	1069:1083	arg1	capacity					1085:1092	water-retention capacity	1069:1092	water-retention capacity	1069:1092	The pasting viscosity, swelling degree of starch granules, viscoelasticity, gel strength, cold storage stability and water-retention capacity of KBST were enhanced with increasing AP concentration.					
34560153	1	75	theme	multiple	246:253	arg1	bioactivities					255:267	multiple bioactivities	246:267	multiple bioactivities	246:267	Auricularia auricula-judae polysaccharide (AP) has unique molecular structures and multiple bioactivities with excellent gel-forming property and thermal tolerance.					
34560153	4	76	theme	pasting	577:583	arg1	properties					609:618	The pasting, gelling and structural properties	573:618	The pasting, gelling and structural properties of AP-KBST mixtures	573:638	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	77	theme	visco	668:672	arg1	analyzer					728:735	laser particle analyzer	713:735	laser particle analyzer	713:735	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	77	theme	visco	668:672	arg1	rheometry					684:692	rheometry	684:692	rheometry	684:692	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	77	theme	visco	668:672	arg1	analyzer					703:710	texture analyzer	695:710	texture analyzer	695:710	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	77	theme	visco	668:672	arg1	analyzer					674:681	rapid visco analyzer	662:681	rapid visco analyzer	662:681	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	4	77	theme	visco	668:672	arg1	resonance					765:773	low-field nuclear magnetic resonance	738:773	low-field nuclear magnetic resonance	738:773	The pasting, gelling and structural properties of AP-KBST mixtures were characterized by rapid visco analyzer, rheometry, texture analyzer, laser particle analyzer, low-field nuclear magnetic resonance, Fourier transform infrared spectroscopy and scanning electron microscopy, respectively.					
34560153	7	78	theme	denser	1237:1242	arg1	structure					1244:1252	and a firmer and denser structure	1220:1252	structure	1244:1252	The combination of AP and KBST exhibited a higher short-range ordered and a firmer and denser structure than that of KBST alone.					
33426826	0	0	theme	Chinese	97:103	arg1	OCS02®					160:165	Ophiocordyceps sinensis Cultivar OCS02®	127:165	Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes)	127:179	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	0	0	theme	Chinese	97:103	arg1	Mushroom					117:124	Chinese Caterpillar Mushroom	97:124	Chinese Caterpillar Mushroom	97:124	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	7	1	theme	immunomodulatory	1341:1356	arg1	effect					1358:1363	their immunomodulatory effect	1335:1363	their immunomodulatory effect	1335:1363	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions is needed to delineate the underlying mechanism of their immunomodulatory effect both in vitro and in vivo.					
33426826	4	2	from	Polysaccharides	654:668	arg1	extract					677:683	extract	677:683	extract	677:683	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	2	from	Polysaccharides	654:668	arg1	fractions					689:697	fractions	689:697	fractions	689:697	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	6	3	theme	MIP-2	1042:1046	arg1	modulation					1016:1025	significant modulation	1004:1025	significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein	1004:1172	All treatments showed significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein.					
33426826	4	4	theme	xylose	826:831	arg1	galactose					792:800	galactose	792:800	galactose	792:800	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	4	theme	xylose	826:831	arg1	amounts					781:787	small amounts	775:787	small amounts of galactose, fucose, arabinose, and xylose	775:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	4	theme	xylose	826:831	arg1	arabinose					811:819	arabinose	811:819	arabinose	811:819	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	4	theme	xylose	826:831	arg1	xylose					826:831	xylose	826:831	xylose	826:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	4	theme	xylose	826:831	arg1	fucose					803:808	fucose	803:808	fucose	803:808	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	1	5	theme	Ophiocordyceps	182:195	arg1	sinensis					197:204	Ophiocordyceps sinensis	182:204	Ophiocordyceps sinensis (=Cordyceps sinensis)	182:226	Ophiocordyceps sinensis (=Cordyceps sinensis) has been known for its various medicinal properties, in particular immunomodulatory activities associated with its polysaccharides.					
33426826	1	5	theme	Ophiocordyceps	182:195	arg1	sinensis					218:225	=Cordyceps sinensis	207:225	=Cordyceps sinensis	207:225	Ophiocordyceps sinensis (=Cordyceps sinensis) has been known for its various medicinal properties, in particular immunomodulatory activities associated with its polysaccharides.					
33426826	0	6	theme	Mushroom	117:124	arg1	Body					89:92	the Fruiting Body	76:92	the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes)	76:179	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	6	7	theme	presence	1154:1161	arg1	ratios					1128:1133	their high ratios	1117:1133	their high ratios of glucose and the presence of protein	1117:1172	All treatments showed significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein.					
33426826	5	8	theme	murine	953:958	arg1	RAW					971:973	murine macrophage RAW 264.7	953:979	murine macrophage RAW 264.7	953:979	The immunomodulatory potential of these heteroglycans was evaluated by induction of cytokine/chemokine secretion using murine macrophage RAW 264.7.					
33426826	6	9	theme	protein	1166:1172	arg1	glucose					1138:1144	glucose	1138:1144	glucose	1138:1144	All treatments showed significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein.					
33426826	6	9	theme	protein	1166:1172	arg1	presence					1154:1161	the presence	1150:1161	the presence of protein	1150:1172	All treatments showed significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein.					
33426826	0	10	theme	Caterpillar	105:115	arg1	OCS02®					160:165	Ophiocordyceps sinensis Cultivar OCS02®	127:165	Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes)	127:179	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	0	10	theme	Caterpillar	105:115	arg1	Mushroom					117:124	Chinese Caterpillar Mushroom	97:124	Chinese Caterpillar Mushroom	97:124	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	7	11	theme	body	1254:1257	arg1	fractions					1274:1282	these fruiting body polysaccharide fractions	1239:1282	these fruiting body polysaccharide fractions	1239:1282	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions is needed to delineate the underlying mechanism of their immunomodulatory effect both in vitro and in vivo.					
33426826	5	12	theme	macrophage	960:969	arg1	RAW					971:973	murine macrophage RAW 264.7	953:979	murine macrophage RAW 264.7	953:979	The immunomodulatory potential of these heteroglycans was evaluated by induction of cytokine/chemokine secretion using murine macrophage RAW 264.7.					
33426826	7	13	theme	Further	1175:1181	arg1	investigation					1183:1195	Further investigation	1175:1195	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions	1175:1282	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions is needed to delineate the underlying mechanism of their immunomodulatory effect both in vitro and in vivo.					
33426826	3	14	theme	molecular	576:584	arg1	MW					594:595	MW	594:595	MW	594:595	Cold water extract (CWE) obtained from this fruiting body was fractionated by molecular weight (MW) into high (HMW), medium (MMW), and low (LMW) fractions.					
33426826	3	14	theme	molecular	576:584	arg1	weight					586:591	molecular weight	576:591	molecular weight (MW)	576:596	Cold water extract (CWE) obtained from this fruiting body was fractionated by molecular weight (MW) into high (HMW), medium (MMW), and low (LMW) fractions.					
33426826	1	15	theme	particular	284:293	arg1	activities					312:321	particular immunomodulatory activities	284:321	particular immunomodulatory activities associated with its polysaccharides	284:357	Ophiocordyceps sinensis (=Cordyceps sinensis) has been known for its various medicinal properties, in particular immunomodulatory activities associated with its polysaccharides.					
33426826	0	16	theme	Ophiocordyceps	127:140	arg1	OCS02®					160:165	Ophiocordyceps sinensis Cultivar OCS02®	127:165	Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes)	127:179	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	0	16	theme	Ophiocordyceps	127:140	arg1	Mushroom					117:124	Chinese Caterpillar Mushroom	97:124	Chinese Caterpillar Mushroom	97:124	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	0	16	theme	Ophiocordyceps	127:140	arg1	Ascomycetes					168:178	Ascomycetes	168:178	Ascomycetes	168:178	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	4	17	theme	arabinose	811:819	arg1	galactose					792:800	galactose	792:800	galactose	792:800	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	17	theme	arabinose	811:819	arg1	amounts					781:787	small amounts	775:787	small amounts of galactose, fucose, arabinose, and xylose	775:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	17	theme	arabinose	811:819	arg1	arabinose					811:819	arabinose	811:819	arabinose	811:819	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	17	theme	arabinose	811:819	arg1	xylose					826:831	xylose	826:831	xylose	826:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	17	theme	arabinose	811:819	arg1	fucose					803:808	fucose	803:808	fucose	803:808	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	7	18	theme	polysaccharide	1259:1272	arg1	fractions					1274:1282	these fruiting body polysaccharide fractions	1239:1282	these fruiting body polysaccharide fractions	1239:1282	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions is needed to delineate the underlying mechanism of their immunomodulatory effect both in vitro and in vivo.					
33426826	1	19	theme	=Cordyceps	207:216	arg1	sinensis					197:204	Ophiocordyceps sinensis	182:204	Ophiocordyceps sinensis (=Cordyceps sinensis)	182:226	Ophiocordyceps sinensis (=Cordyceps sinensis) has been known for its various medicinal properties, in particular immunomodulatory activities associated with its polysaccharides.					
33426826	1	19	theme	=Cordyceps	207:216	arg1	sinensis					218:225	=Cordyceps sinensis	207:225	=Cordyceps sinensis	207:225	Ophiocordyceps sinensis (=Cordyceps sinensis) has been known for its various medicinal properties, in particular immunomodulatory activities associated with its polysaccharides.					
33426826	4	20	with	mannose	762:768	arg1	galactose					792:800	galactose	792:800	galactose	792:800	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	20	with	mannose	762:768	arg1	amounts					781:787	small amounts	775:787	small amounts of galactose, fucose, arabinose, and xylose	775:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	20	with	mannose	762:768	arg1	arabinose					811:819	arabinose	811:819	arabinose	811:819	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	20	with	mannose	762:768	arg1	xylose					826:831	xylose	826:831	xylose	826:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	20	with	mannose	762:768	arg1	fucose					803:808	fucose	803:808	fucose	803:808	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	5	21	theme	heteroglycans	874:886	arg1	potential					855:863	The immunomodulatory potential	834:863	The immunomodulatory potential of these heteroglycans	834:886	The immunomodulatory potential of these heteroglycans was evaluated by induction of cytokine/chemokine secretion using murine macrophage RAW 264.7.					
33426826	0	22	theme	Immunomodulatory	0:15	arg1	Properties					17:26	Immunomodulatory Properties	0:26	Immunomodulatory Properties of Water-Soluble Polysaccharides	0:59	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	2	23	theme	OCS02®	417:422	arg1	body					388:391	the fruiting body	375:391	the fruiting body of O. sinensis cultivar OCS02®	375:422	In this study, the fruiting body of O. sinensis cultivar OCS02® was investigated for its chemical composition and monosaccharide profile.					
33426826	2	24	theme	cultivar	408:415	arg1	OCS02®					417:422	O. sinensis cultivar OCS02®	396:422	O. sinensis cultivar OCS02®	396:422	In this study, the fruiting body of O. sinensis cultivar OCS02® was investigated for its chemical composition and monosaccharide profile.					
33426826	2	25	theme	monosaccharide	474:487	arg1	profile					489:495	monosaccharide profile	474:495	monosaccharide profile	474:495	In this study, the fruiting body of O. sinensis cultivar OCS02® was investigated for its chemical composition and monosaccharide profile.					
33426826	1	26	theme	immunomodulatory	295:310	arg1	activities					312:321	particular immunomodulatory activities	284:321	particular immunomodulatory activities associated with its polysaccharides	284:357	Ophiocordyceps sinensis (=Cordyceps sinensis) has been known for its various medicinal properties, in particular immunomodulatory activities associated with its polysaccharides.					
33426826	5	27	theme	cytokine/chemokine	918:935	arg1	secretion					937:945	cytokine/chemokine secretion	918:945	cytokine/chemokine secretion using murine macrophage RAW 264.7	918:979	The immunomodulatory potential of these heteroglycans was evaluated by induction of cytokine/chemokine secretion using murine macrophage RAW 264.7.					
33426826	2	28	theme	sinensis	399:406	arg1	OCS02®					417:422	O. sinensis cultivar OCS02®	396:422	O. sinensis cultivar OCS02®	396:422	In this study, the fruiting body of O. sinensis cultivar OCS02® was investigated for its chemical composition and monosaccharide profile.					
33426826	0	29	theme	Water-Soluble	31:43	arg1	Polysaccharides					45:59	Water-Soluble Polysaccharides	31:59	Water-Soluble Polysaccharides	31:59	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	4	30	with	glucose	750:756	arg1	galactose					792:800	galactose	792:800	galactose	792:800	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	30	with	glucose	750:756	arg1	amounts					781:787	small amounts	775:787	small amounts of galactose, fucose, arabinose, and xylose	775:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	30	with	glucose	750:756	arg1	arabinose					811:819	arabinose	811:819	arabinose	811:819	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	30	with	glucose	750:756	arg1	xylose					826:831	xylose	826:831	xylose	826:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	30	with	glucose	750:756	arg1	fucose					803:808	fucose	803:808	fucose	803:808	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	0	31	theme	Cultivar	151:158	arg1	OCS02®					160:165	Ophiocordyceps sinensis Cultivar OCS02®	127:165	Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes)	127:179	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	0	31	theme	Cultivar	151:158	arg1	Mushroom					117:124	Chinese Caterpillar Mushroom	97:124	Chinese Caterpillar Mushroom	97:124	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	0	31	theme	Cultivar	151:158	arg1	Ascomycetes					168:178	Ascomycetes	168:178	Ascomycetes	168:178	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	4	32	dep	extract	677:683	arg1	the					673:675	the	673:675	the	673:675	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	6	33	theme	TIMP-1	1053:1058	arg1	modulation					1016:1025	significant modulation	1004:1025	significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein	1004:1172	All treatments showed significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein.					
33426826	2	34	theme	chemical	449:456	arg1	composition					458:468	its chemical composition	445:468	its chemical composition	445:468	In this study, the fruiting body of O. sinensis cultivar OCS02® was investigated for its chemical composition and monosaccharide profile.					
33426826	0	35	theme	sinensis	142:149	arg1	OCS02®					160:165	Ophiocordyceps sinensis Cultivar OCS02®	127:165	Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes)	127:179	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	0	35	theme	sinensis	142:149	arg1	Mushroom					117:124	Chinese Caterpillar Mushroom	97:124	Chinese Caterpillar Mushroom	97:124	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	0	35	theme	sinensis	142:149	arg1	Ascomycetes					168:178	Ascomycetes	168:178	Ascomycetes	168:178	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	6	36	theme	high	1123:1126	arg1	ratios					1128:1133	their high ratios	1117:1133	their high ratios of glucose and the presence of protein	1117:1172	All treatments showed significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein.					
33426826	2	37	theme	fruiting	379:386	arg1	body					388:391	the fruiting body	375:391	the fruiting body of O. sinensis cultivar OCS02®	375:422	In this study, the fruiting body of O. sinensis cultivar OCS02® was investigated for its chemical composition and monosaccharide profile.					
33426826	3	38	theme	fruiting	542:549	arg1	body					551:554	this fruiting body	537:554	this fruiting body	537:554	Cold water extract (CWE) obtained from this fruiting body was fractionated by molecular weight (MW) into high (HMW), medium (MMW), and low (LMW) fractions.					
33426826	4	39	theme	galactose	792:800	arg1	galactose					792:800	galactose	792:800	galactose	792:800	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	39	theme	galactose	792:800	arg1	amounts					781:787	small amounts	775:787	small amounts of galactose, fucose, arabinose, and xylose	775:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	39	theme	galactose	792:800	arg1	arabinose					811:819	arabinose	811:819	arabinose	811:819	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	39	theme	galactose	792:800	arg1	xylose					826:831	xylose	826:831	xylose	826:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	39	theme	galactose	792:800	arg1	fucose					803:808	fucose	803:808	fucose	803:808	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	5	40	theme	immunomodulatory	838:853	arg1	potential					855:863	The immunomodulatory potential	834:863	The immunomodulatory potential of these heteroglycans	834:886	The immunomodulatory potential of these heteroglycans was evaluated by induction of cytokine/chemokine secretion using murine macrophage RAW 264.7.					
33426826	6	41	theme	IL-9	1036:1039	arg1	modulation					1016:1025	significant modulation	1004:1025	significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein	1004:1172	All treatments showed significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein.					
33426826	0	42	theme	Polysaccharides	45:59	arg1	Properties					17:26	Immunomodulatory Properties	0:26	Immunomodulatory Properties of Water-Soluble Polysaccharides	0:59	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	6	43	theme	significant	1004:1014	arg1	modulation					1016:1025	significant modulation	1004:1025	significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein	1004:1172	All treatments showed significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein.					
33426826	3	44	theme	Cold	498:501	arg1	CWE					518:520	CWE	518:520	CWE	518:520	Cold water extract (CWE) obtained from this fruiting body was fractionated by molecular weight (MW) into high (HMW), medium (MMW), and low (LMW) fractions.					
33426826	3	44	theme	Cold	498:501	arg1	extract					509:515	Cold water extract	498:515	Cold water extract (CWE) obtained from this fruiting body	498:554	Cold water extract (CWE) obtained from this fruiting body was fractionated by molecular weight (MW) into high (HMW), medium (MMW), and low (LMW) fractions.					
33426826	4	45	theme	fucose	803:808	arg1	galactose					792:800	galactose	792:800	galactose	792:800	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	45	theme	fucose	803:808	arg1	amounts					781:787	small amounts	775:787	small amounts of galactose, fucose, arabinose, and xylose	775:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	45	theme	fucose	803:808	arg1	arabinose					811:819	arabinose	811:819	arabinose	811:819	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	45	theme	fucose	803:808	arg1	xylose					826:831	xylose	826:831	xylose	826:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	45	theme	fucose	803:808	arg1	fucose					803:808	fucose	803:808	fucose	803:808	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	46	contain	containing	732:741	arg1	Polysaccharides					654:668	Polysaccharides	654:668	Polysaccharides in the extract and fractions	654:697	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	46	contain	containing	732:741	arg2	mannose					762:768	mannose	762:768	mannose	762:768	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	46	contain	containing	732:741	arg1	heteroglycans					718:730	heteroglycans	718:730	heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose	718:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	46	contain	containing	732:741	arg2	glucose					750:756	glucose	750:756	glucose	750:756	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	7	47	from	investigation	1183:1195	arg1	relationship					1223:1234	the structure-function relationship	1200:1234	the structure-function relationship of these fruiting body polysaccharide fractions	1200:1282	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions is needed to delineate the underlying mechanism of their immunomodulatory effect both in vitro and in vivo.					
33426826	7	48	theme	underlying	1311:1320	arg1	mechanism					1322:1330	the underlying mechanism	1307:1330	the underlying mechanism of their immunomodulatory effect	1307:1363	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions is needed to delineate the underlying mechanism of their immunomodulatory effect both in vitro and in vivo.					
33426826	7	49	theme	effect	1358:1363	arg1	mechanism					1322:1330	the underlying mechanism	1307:1330	the underlying mechanism of their immunomodulatory effect	1307:1363	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions is needed to delineate the underlying mechanism of their immunomodulatory effect both in vitro and in vivo.					
33426826	5	50	theme	secretion	937:945	arg1	induction					905:913	induction	905:913	induction of cytokine/chemokine secretion using murine macrophage RAW 264.7	905:979	The immunomodulatory potential of these heteroglycans was evaluated by induction of cytokine/chemokine secretion using murine macrophage RAW 264.7.					
33426826	6	51	theme	IL-6	1030:1033	arg1	modulation					1016:1025	significant modulation	1004:1025	significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein	1004:1172	All treatments showed significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein.					
33426826	6	52	theme	glucose	1138:1144	arg1	ratios					1128:1133	their high ratios	1117:1133	their high ratios of glucose and the presence of protein	1117:1172	All treatments showed significant modulation of IL-6, IL-9, MIP-2, and TIMP-1, especially for CWE, HMW, and MMW, which might be due to their high ratios of glucose and the presence of protein.					
33426826	7	53	theme	structure-function	1204:1221	arg1	relationship					1223:1234	the structure-function relationship	1200:1234	the structure-function relationship of these fruiting body polysaccharide fractions	1200:1282	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions is needed to delineate the underlying mechanism of their immunomodulatory effect both in vitro and in vivo.					
33426826	7	54	theme	fractions	1274:1282	arg1	relationship					1223:1234	the structure-function relationship	1200:1234	the structure-function relationship of these fruiting body polysaccharide fractions	1200:1282	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions is needed to delineate the underlying mechanism of their immunomodulatory effect both in vitro and in vivo.					
33426826	7	55	theme	fruiting	1245:1252	arg1	fractions					1274:1282	these fruiting body polysaccharide fractions	1239:1282	these fruiting body polysaccharide fractions	1239:1282	Further investigation on the structure-function relationship of these fruiting body polysaccharide fractions is needed to delineate the underlying mechanism of their immunomodulatory effect both in vitro and in vivo.					
33426826	3	56	theme	water	503:507	arg1	CWE					518:520	CWE	518:520	CWE	518:520	Cold water extract (CWE) obtained from this fruiting body was fractionated by molecular weight (MW) into high (HMW), medium (MMW), and low (LMW) fractions.					
33426826	3	56	theme	water	503:507	arg1	extract					509:515	Cold water extract	498:515	Cold water extract (CWE) obtained from this fruiting body	498:554	Cold water extract (CWE) obtained from this fruiting body was fractionated by molecular weight (MW) into high (HMW), medium (MMW), and low (LMW) fractions.					
33426826	2	57	theme	O.	396:397	arg1	OCS02®					417:422	O. sinensis cultivar OCS02®	396:422	O. sinensis cultivar OCS02®	396:422	In this study, the fruiting body of O. sinensis cultivar OCS02® was investigated for its chemical composition and monosaccharide profile.					
33426826	3	58	dep	high	603:606	arg1	fractions					643:651	fractions	643:651	fractions	643:651	Cold water extract (CWE) obtained from this fruiting body was fractionated by molecular weight (MW) into high (HMW), medium (MMW), and low (LMW) fractions.					
33426826	1	59	theme	various	251:257	arg1	properties					269:278	its various medicinal properties	247:278	its various medicinal properties	247:278	Ophiocordyceps sinensis (=Cordyceps sinensis) has been known for its various medicinal properties, in particular immunomodulatory activities associated with its polysaccharides.					
33426826	0	60	theme	Fruiting	80:87	arg1	Body					89:92	the Fruiting Body	76:92	the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes)	76:179	Immunomodulatory Properties of Water-Soluble Polysaccharides Extracted from the Fruiting Body of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis Cultivar OCS02® (Ascomycetes).					
33426826	4	61	theme	small	775:779	arg1	galactose					792:800	galactose	792:800	galactose	792:800	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	61	theme	small	775:779	arg1	amounts					781:787	small amounts	775:787	small amounts of galactose, fucose, arabinose, and xylose	775:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	61	theme	small	775:779	arg1	arabinose					811:819	arabinose	811:819	arabinose	811:819	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	61	theme	small	775:779	arg1	xylose					826:831	xylose	826:831	xylose	826:831	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	4	61	theme	small	775:779	arg1	fucose					803:808	fucose	803:808	fucose	803:808	Polysaccharides in the extract and fractions were identified as heteroglycans containing mostly glucose and mannose with small amounts of galactose, fucose, arabinose, and xylose.					
33426826	1	62	theme	medicinal	259:267	arg1	properties					269:278	its various medicinal properties	247:278	its various medicinal properties	247:278	Ophiocordyceps sinensis (=Cordyceps sinensis) has been known for its various medicinal properties, in particular immunomodulatory activities associated with its polysaccharides.					
32404017	4	0	theme	microbiome	606:615	arg1	shifts					617:622	small intestinal microbiome shifts	589:622	small intestinal microbiome shifts	589:622	As a result, the functional significance of small intestinal microbiome shifts during infection is not well defined.					
32404017	9	1	theme	mucin-producing	1435:1449	arg1	lines					1456:1460	mucin-producing cell lines	1435:1460	mucin-producing cell lines	1435:1460	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	6	2	from	changes	930:936	arg1	response					962:969	response	962:969	response to rotavirus infection	962:992	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	6	2	from	changes	930:936	arg1	communities					947:957	ileal communities	941:957	ileal communities	941:957	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	5	3	theme	rotavirus-infected	685:702	arg1	mice					713:716	rotavirus-infected neonatal mice	685:716	rotavirus-infected neonatal mice	685:716	To address these gaps, rotavirus-infected neonatal mice were examined for changes in bacterial community dynamics, host gene expression, and tissue recovery during infection.					
32404017	6	4	theme	Profiling	837:845	arg1	communities					857:867	Profiling bacterial communities	837:867	Profiling bacterial communities using 16S rRNA sequencing	837:893	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	9	5	theme	lines	1456:1460	arg1	infection					1422:1430	Rotavirus infection	1412:1430	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs)	1412:1498	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	6	6	theme	ileal	941:945	arg1	communities					947:957	ileal communities	941:957	ileal communities	941:957	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	3	7	dep	this	354:357	arg1	Compounding					342:352	Compounding	342:352	Compounding	342:352	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	9	8	theme	human	1466:1470	arg1	HIEs					1494:1497	HIEs	1494:1497	HIEs	1494:1497	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	9	8	theme	human	1466:1470	arg1	enteroids					1483:1491	human intestinal enteroids	1466:1491	human intestinal enteroids (HIEs)	1466:1498	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	9	9	theme	enteroids	1483:1491	arg1	infection					1422:1430	Rotavirus infection	1412:1430	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs)	1412:1498	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	7	10	theme	Lactobacillus	1114:1126	arg1	species					1128:1134	Lactobacillus species	1114:1134	Lactobacillus species from the ileum	1114:1149	At 1-d post-infection, we observed a loss in Lactobacillus species from the ileum, but an increase in Bacteroides and Akkermansia, both of which exhibit mucin-digesting capabilities.					
32404017	1	11	theme	bacterial	229:237	arg1	pathogens					239:247	diarrheal-inducing bacterial pathogens	210:247	diarrheal-inducing bacterial pathogens	210:247	Multiple studies have identified changes within the gut microbiome in response to diarrheal-inducing bacterial pathogens.					
32404017	6	12	theme	significant	1003:1013	arg1	changes					1015:1021	no significant changes	1000:1021	no significant changes for other gastrointestinal (GI) compartments	1000:1066	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	7	13	from	loss	1106:1109	arg1	species					1128:1134	Lactobacillus species	1114:1134	Lactobacillus species from the ileum	1114:1149	At 1-d post-infection, we observed a loss in Lactobacillus species from the ileum, but an increase in Bacteroides and Akkermansia, both of which exhibit mucin-digesting capabilities.					
32404017	7	13	from	loss	1106:1109	arg1	ileum					1145:1149	the ileum	1141:1149	the ileum	1141:1149	At 1-d post-infection, we observed a loss in Lactobacillus species from the ileum, but an increase in Bacteroides and Akkermansia, both of which exhibit mucin-digesting capabilities.					
32404017	8	14	theme	bacterial	1273:1281	arg1	shifts					1293:1298	the bacterial community shifts	1269:1298	the bacterial community shifts	1269:1298	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	0	15	from	changes	74:80	arg1	microbiome					89:98	the microbiome	85:98	the microbiome	85:98	Rotavirus infection induces glycan availability to promote ileum-specific changes in the microbiome aiding rotavirus virulence.					
32404017	8	16	theme	goblet	1336:1341	arg1	cells					1343:1347	mucin-filled goblet cells	1323:1347	mucin-filled goblet cells in the small intestine at d 1	1323:1377	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	9	17	dep	in	1556:1557	arg1	vivo					1559:1562	vivo	1559:1562	vivo	1559:1562	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	9	18	theme	mucin	1529:1533	arg1	granules					1535:1542	stored mucin granules	1522:1542	stored mucin granules	1522:1542	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	8	19	theme	small	1356:1360	arg1	intestine					1362:1370	the small intestine	1352:1370	the small intestine at d 1	1352:1377	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	1	20	from	microbiome	184:193	arg1	response					198:205	response	198:205	response to diarrheal-inducing bacterial pathogens	198:247	Multiple studies have identified changes within the gut microbiome in response to diarrheal-inducing bacterial pathogens.					
32404017	6	21	dep	gastrointestinal	1033:1048	arg1	GI					1051:1052	GI	1051:1052	GI	1051:1052	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	8	22	with	Concomitant	1252:1262	arg1	shifts					1293:1298	the bacterial community shifts	1269:1298	the bacterial community shifts	1269:1298	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	1	23	theme	gut	180:182	arg1	microbiome					184:193	the gut microbiome	176:193	the gut microbiome in response to diarrheal-inducing bacterial pathogens	176:247	Multiple studies have identified changes within the gut microbiome in response to diarrheal-inducing bacterial pathogens.					
32404017	6	24	theme	gastrointestinal	1033:1048	arg1	compartments					1055:1066	other gastrointestinal (GI) compartments	1027:1066	other gastrointestinal (GI) compartments	1027:1066	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	3	25	theme	fecal	377:381	arg1	samples					383:389	fecal samples	377:389	fecal samples	377:389	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	5	26	from	changes	736:742	arg1	dynamics					767:774	bacterial community dynamics	747:774	bacterial community dynamics	747:774	To address these gaps, rotavirus-infected neonatal mice were examined for changes in bacterial community dynamics, host gene expression, and tissue recovery during infection.					
32404017	5	27	theme	host	777:780	arg1	expression					787:796	host gene expression	777:796	host gene expression	777:796	To address these gaps, rotavirus-infected neonatal mice were examined for changes in bacterial community dynamics, host gene expression, and tissue recovery during infection.					
32404017	10	28	theme	significant	1657:1667	arg1	degradation					1676:1686	significant glycan degradation	1657:1686	significant glycan degradation	1657:1686	In vitro, incubation of mucins with Bacteroides or Akkermansia members resulted in significant glycan degradation, which altered the binding capacity of rotavirus in silico and in vitro.					
32404017	0	29	theme	Rotavirus	0:8	arg1	infection					10:18	Rotavirus infection	0:18	Rotavirus infection	0:18	Rotavirus infection induces glycan availability to promote ileum-specific changes in the microbiome aiding rotavirus virulence.					
32404017	3	30	theme	microbiome	401:410	arg1	composition					412:422	microbiome composition	401:422	microbiome composition	401:422	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	4	31	theme	functional	562:571	arg1	significance					573:584	the functional significance	558:584	the functional significance of small intestinal microbiome shifts during infection	558:639	As a result, the functional significance of small intestinal microbiome shifts during infection is not well defined.					
32404017	4	31	theme	functional	562:571	arg1	result					550:555	a result	548:555	a result	548:555	As a result, the functional significance of small intestinal microbiome shifts during infection is not well defined.					
32404017	6	32	theme	distinct	921:928	arg1	changes					930:936	significant and distinct changes	905:936	significant and distinct changes in ileal communities in response to rotavirus infection	905:992	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	7	33	from	ileum	1145:1149	arg1	loss					1106:1109	a loss	1104:1109	a loss in Lactobacillus species from the ileum	1104:1149	At 1-d post-infection, we observed a loss in Lactobacillus species from the ileum, but an increase in Bacteroides and Akkermansia, both of which exhibit mucin-digesting capabilities.					
32404017	7	33	from	ileum	1145:1149	arg1	species					1128:1134	Lactobacillus species	1114:1134	Lactobacillus species from the ileum	1114:1149	At 1-d post-infection, we observed a loss in Lactobacillus species from the ileum, but an increase in Bacteroides and Akkermansia, both of which exhibit mucin-digesting capabilities.					
32404017	6	34	theme	significant	905:915	arg1	changes					930:936	significant and distinct changes	905:936	significant and distinct changes in ileal communities in response to rotavirus infection	905:992	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	3	35	theme	most	514:517	arg1	infections					533:542	most enteric virus infections	514:542	most enteric virus infections	514:542	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	7	36	from	increase	1159:1166	arg1	Akkermansia					1187:1197	Akkermansia	1187:1197	Akkermansia	1187:1197	At 1-d post-infection, we observed a loss in Lactobacillus species from the ileum, but an increase in Bacteroides and Akkermansia, both of which exhibit mucin-digesting capabilities.					
32404017	7	36	from	increase	1159:1166	arg1	Bacteroides					1171:1181	Bacteroides	1171:1181	Bacteroides	1171:1181	At 1-d post-infection, we observed a loss in Lactobacillus species from the ileum, but an increase in Bacteroides and Akkermansia, both of which exhibit mucin-digesting capabilities.					
32404017	6	37	theme	16S	875:877	arg1	sequencing					884:893	16S rRNA sequencing	875:893	16S rRNA sequencing	875:893	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	11	38	theme	mucin-degrading	1930:1944	arg1	microbes					1946:1953	mucin-degrading microbes	1930:1953	mucin-degrading microbes	1930:1953	Taken together, these data suggest that the response to and recovery from rotavirus-diarrhea is unique between sub-compartments of the GI tract and may be influenced by mucin-degrading microbes.					
32404017	11	39	theme	GI	1896:1897	arg1	tract					1899:1903	the GI tract	1892:1903	the GI tract	1892:1903	Taken together, these data suggest that the response to and recovery from rotavirus-diarrhea is unique between sub-compartments of the GI tract and may be influenced by mucin-degrading microbes.					
32404017	8	40	from	d	1375:1375	arg1	cells					1343:1347	mucin-filled goblet cells	1323:1347	mucin-filled goblet cells in the small intestine at d 1	1323:1377	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	8	40	from	d	1375:1375	arg1	intestine					1362:1370	the small intestine	1352:1370	the small intestine at d 1	1352:1377	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	9	41	theme	Rotavirus	1412:1420	arg1	infection					1422:1430	Rotavirus infection	1412:1430	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs)	1412:1498	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	6	42	theme	rotavirus	974:982	arg1	infection					984:992	rotavirus infection	974:992	rotavirus infection	974:992	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	3	43	theme	virus	527:531	arg1	infections					533:542	most enteric virus infections	514:542	most enteric virus infections	514:542	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	4	44	theme	intestinal	595:604	arg1	shifts					617:622	small intestinal microbiome shifts	589:622	small intestinal microbiome shifts	589:622	As a result, the functional significance of small intestinal microbiome shifts during infection is not well defined.					
32404017	6	45	theme	bacterial	847:855	arg1	communities					857:867	Profiling bacterial communities	837:867	Profiling bacterial communities using 16S rRNA sequencing	837:893	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	9	46	theme	cell	1451:1454	arg1	lines					1456:1460	mucin-producing cell lines	1435:1460	mucin-producing cell lines	1435:1460	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	11	47	from	rotavirus-diarrhea	1835:1852	arg1	recovery					1821:1828	recovery	1821:1828	recovery from rotavirus-diarrhea	1821:1852	Taken together, these data suggest that the response to and recovery from rotavirus-diarrhea is unique between sub-compartments of the GI tract and may be influenced by mucin-degrading microbes.					
32404017	4	48	theme	shifts	617:622	arg1	significance					573:584	the functional significance	558:584	the functional significance of small intestinal microbiome shifts during infection	558:639	As a result, the functional significance of small intestinal microbiome shifts during infection is not well defined.					
32404017	4	48	theme	shifts	617:622	arg1	result					550:555	a result	548:555	a result	548:555	As a result, the functional significance of small intestinal microbiome shifts during infection is not well defined.					
32404017	10	49	theme	rotavirus	1727:1735	arg1	capacity					1715:1722	the binding capacity	1703:1722	the binding capacity of rotavirus	1703:1735	In vitro, incubation of mucins with Bacteroides or Akkermansia members resulted in significant glycan degradation, which altered the binding capacity of rotavirus in silico and in vitro.					
32404017	2	50	theme	viral	304:308	arg1	pathogens					310:318	viral pathogens	304:318	viral pathogens	304:318	However, examination of the microbiome in response to viral pathogens remains understudied.					
32404017	8	51	theme	community	1283:1291	arg1	shifts					1293:1298	the bacterial community shifts	1269:1298	the bacterial community shifts	1269:1298	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	10	52	theme	Bacteroides	1610:1620	arg1	members					1637:1643	Bacteroides or Akkermansia members	1610:1643	members	1637:1643	In vitro, incubation of mucins with Bacteroides or Akkermansia members resulted in significant glycan degradation, which altered the binding capacity of rotavirus in silico and in vitro.					
32404017	9	53	theme	intestinal	1472:1481	arg1	HIEs					1494:1497	HIEs	1494:1497	HIEs	1494:1497	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	9	53	theme	intestinal	1472:1481	arg1	enteroids					1483:1491	human intestinal enteroids	1466:1491	human intestinal enteroids (HIEs)	1466:1498	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	6	54	theme	other	1027:1031	arg1	compartments					1055:1066	other gastrointestinal (GI) compartments	1027:1066	other gastrointestinal (GI) compartments	1027:1066	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	10	55	with	incubation	1584:1593	arg1	members					1637:1643	Bacteroides or Akkermansia members	1610:1643	members	1637:1643	In vitro, incubation of mucins with Bacteroides or Akkermansia members resulted in significant glycan degradation, which altered the binding capacity of rotavirus in silico and in vitro.					
32404017	10	56	theme	mucins	1598:1603	arg1	incubation					1584:1593	incubation	1584:1593	incubation of mucins with Bacteroides or Akkermansia members	1584:1643	In vitro, incubation of mucins with Bacteroides or Akkermansia members resulted in significant glycan degradation, which altered the binding capacity of rotavirus in silico and in vitro.					
32404017	1	57	theme	diarrheal-inducing	210:227	arg1	pathogens					239:247	diarrheal-inducing bacterial pathogens	210:247	diarrheal-inducing bacterial pathogens	210:247	Multiple studies have identified changes within the gut microbiome in response to diarrheal-inducing bacterial pathogens.					
32404017	10	58	theme	Akkermansia	1625:1635	arg1	members					1637:1643	Bacteroides or Akkermansia members	1610:1643	members	1637:1643	In vitro, incubation of mucins with Bacteroides or Akkermansia members resulted in significant glycan degradation, which altered the binding capacity of rotavirus in silico and in vitro.					
32404017	5	59	theme	community	757:765	arg1	dynamics					767:774	bacterial community dynamics	747:774	bacterial community dynamics	747:774	To address these gaps, rotavirus-infected neonatal mice were examined for changes in bacterial community dynamics, host gene expression, and tissue recovery during infection.					
32404017	5	60	theme	neonatal	704:711	arg1	mice					713:716	rotavirus-infected neonatal mice	685:716	rotavirus-infected neonatal mice	685:716	To address these gaps, rotavirus-infected neonatal mice were examined for changes in bacterial community dynamics, host gene expression, and tissue recovery during infection.					
32404017	7	61	theme	1-d	1072:1074	arg1	post-infection					1076:1089	1-d post-infection	1072:1089	1-d post-infection	1072:1089	At 1-d post-infection, we observed a loss in Lactobacillus species from the ileum, but an increase in Bacteroides and Akkermansia, both of which exhibit mucin-digesting capabilities.					
32404017	1	62	theme	Multiple	128:135	arg1	studies					137:143	Multiple studies	128:143	Multiple studies	128:143	Multiple studies have identified changes within the gut microbiome in response to diarrheal-inducing bacterial pathogens.					
32404017	8	63	theme	mucin-filled	1323:1334	arg1	cells					1343:1347	mucin-filled goblet cells	1323:1347	mucin-filled goblet cells in the small intestine at d 1	1323:1377	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	9	64	theme	stored	1522:1527	arg1	granules					1535:1542	stored mucin granules	1522:1542	stored mucin granules	1522:1542	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	8	65	theme	cells	1343:1347	arg1	loss					1315:1318	a loss	1313:1318	a loss of mucin-filled goblet cells in the small intestine at d 1	1313:1377	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	5	66	theme	gene	782:785	arg1	expression					787:796	host gene expression	777:796	host gene expression	777:796	To address these gaps, rotavirus-infected neonatal mice were examined for changes in bacterial community dynamics, host gene expression, and tissue recovery during infection.					
32404017	5	67	from	expression	787:796	arg1	dynamics					767:774	bacterial community dynamics	747:774	bacterial community dynamics	747:774	To address these gaps, rotavirus-infected neonatal mice were examined for changes in bacterial community dynamics, host gene expression, and tissue recovery during infection.					
32404017	2	68	theme	microbiome	278:287	arg1	examination					259:269	examination	259:269	examination of the microbiome in response to viral pathogens	259:318	However, examination of the microbiome in response to viral pathogens remains understudied.					
32404017	8	69	from	loss	1315:1318	arg1	intestine					1362:1370	the small intestine	1352:1370	the small intestine at d 1	1352:1377	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	9	70	theme	granules	1535:1542	arg1	release					1511:1517	release	1511:1517	release of stored mucin granules	1511:1542	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	3	71	theme	many	360:363	arg1	studies					365:371	many studies	360:371	many studies	360:371	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	0	72	theme	rotavirus	107:115	arg1	virulence					117:125	rotavirus virulence	107:125	rotavirus virulence	107:125	Rotavirus infection induces glycan availability to promote ileum-specific changes in the microbiome aiding rotavirus virulence.					
32404017	5	73	from	recovery	810:817	arg1	dynamics					767:774	bacterial community dynamics	747:774	bacterial community dynamics	747:774	To address these gaps, rotavirus-infected neonatal mice were examined for changes in bacterial community dynamics, host gene expression, and tissue recovery during infection.					
32404017	8	74	from	cells	1343:1347	arg1	d					1375:1375	d 1	1375:1377	d 1	1375:1377	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	8	74	from	cells	1343:1347	arg1	intestine					1362:1370	the small intestine	1352:1370	the small intestine at d 1	1352:1377	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	9	75	theme	in	1556:1557	arg1	findings					1564:1571	in vivo findings	1556:1571	in vivo findings	1556:1571	Rotavirus infection of mucin-producing cell lines and human intestinal enteroids (HIEs) stimulated release of stored mucin granules, similar to in vivo findings.					
32404017	10	76	theme	glycan	1669:1674	arg1	degradation					1676:1686	significant glycan degradation	1657:1686	significant glycan degradation	1657:1686	In vitro, incubation of mucins with Bacteroides or Akkermansia members resulted in significant glycan degradation, which altered the binding capacity of rotavirus in silico and in vitro.					
32404017	8	77	from	intestine	1362:1370	arg1	loss					1315:1318	a loss	1313:1318	a loss of mucin-filled goblet cells in the small intestine at d 1	1313:1377	Concomitant with the bacterial community shifts, we observed a loss of mucin-filled goblet cells in the small intestine at d 1, with recovery occurring by d 3.					
32404017	2	78	from	examination	259:269	arg1	response					292:299	response	292:299	response to viral pathogens	292:318	However, examination of the microbiome in response to viral pathogens remains understudied.					
32404017	0	79	theme	glycan	28:33	arg1	availability					35:46	glycan availability	28:46	glycan availability	28:46	Rotavirus infection induces glycan availability to promote ileum-specific changes in the microbiome aiding rotavirus virulence.					
32404017	4	80	theme	small	589:593	arg1	shifts					617:622	small intestinal microbiome shifts	589:622	small intestinal microbiome shifts	589:622	As a result, the functional significance of small intestinal microbiome shifts during infection is not well defined.					
32404017	3	81	theme	primary	497:503	arg1	site					505:508	the primary site	493:508	the primary site for most enteric virus infections	493:542	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	3	81	theme	primary	497:503	arg1	intestine					482:490	the small intestine	472:490	the small intestine	472:490	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	5	82	theme	tissue	803:808	arg1	recovery					810:817	tissue recovery	803:817	tissue recovery	803:817	To address these gaps, rotavirus-infected neonatal mice were examined for changes in bacterial community dynamics, host gene expression, and tissue recovery during infection.					
32404017	7	83	theme	mucin-digesting	1222:1236	arg1	capabilities					1238:1249	mucin-digesting capabilities	1222:1249	mucin-digesting capabilities	1222:1249	At 1-d post-infection, we observed a loss in Lactobacillus species from the ileum, but an increase in Bacteroides and Akkermansia, both of which exhibit mucin-digesting capabilities.					
32404017	0	84	theme	ileum-specific	59:72	arg1	changes					74:80	ileum-specific changes	59:80	ileum-specific changes in the microbiome aiding rotavirus virulence	59:125	Rotavirus infection induces glycan availability to promote ileum-specific changes in the microbiome aiding rotavirus virulence.					
32404017	5	85	theme	bacterial	747:755	arg1	dynamics					767:774	bacterial community dynamics	747:774	bacterial community dynamics	747:774	To address these gaps, rotavirus-infected neonatal mice were examined for changes in bacterial community dynamics, host gene expression, and tissue recovery during infection.					
32404017	3	86	theme	enteric	519:525	arg1	infections					533:542	most enteric virus infections	514:542	most enteric virus infections	514:542	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	6	87	theme	rRNA	879:882	arg1	sequencing					884:893	16S rRNA sequencing	875:893	16S rRNA sequencing	875:893	Profiling bacterial communities using 16S rRNA sequencing suggested significant and distinct changes in ileal communities in response to rotavirus infection, with no significant changes for other gastrointestinal (GI) compartments.					
32404017	10	88	theme	binding	1707:1713	arg1	capacity					1715:1722	the binding capacity	1703:1722	the binding capacity of rotavirus	1703:1735	In vitro, incubation of mucins with Bacteroides or Akkermansia members resulted in significant glycan degradation, which altered the binding capacity of rotavirus in silico and in vitro.					
32404017	3	89	theme	small	476:480	arg1	site					505:508	the primary site	493:508	the primary site for most enteric virus infections	493:542	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	3	89	theme	small	476:480	arg1	intestine					482:490	the small intestine	472:490	the small intestine	472:490	Compounding this, many studies use fecal samples to assess microbiome composition; which may not accurately mirror changes within the small intestine, the primary site for most enteric virus infections.					
32404017	11	90	theme	tract	1899:1903	arg1	sub-compartments					1872:1887	sub-compartments	1872:1887	sub-compartments of the GI tract	1872:1903	Taken together, these data suggest that the response to and recovery from rotavirus-diarrhea is unique between sub-compartments of the GI tract and may be influenced by mucin-degrading microbes.					
33551164	2	0	theme	same	456:459	arg1	conditions					461:470	the same conditions	452:470	the same conditions	452:470	We evaluated the EPS production capacity of 20 strains of L. paracasei under the same conditions.					
33551164	1	1	theme	source	311:316	arg1	effect					289:294	the effect	285:294	the effect of this carbon source on the production and monosaccharide composition of EPS	285:372	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	9	2	from	role	1677:1680	arg1	production					1707:1716	the production	1703:1716	the production of EPS by L. paracasei TD 062	1703:1746	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	5	3	theme	gene	825:828	arg1	cluster					830:836	an EPS synthesis gene cluster	808:836	an EPS synthesis gene cluster that could participate in transport, export, and sugar chain synthesis, and generate 6 sugar nucleotides	808:941	We identified an EPS synthesis gene cluster that could participate in transport, export, and sugar chain synthesis, and generate 6 sugar nucleotides.					
33551164	1	4	theme	Lactobacillus	237:249	arg1	062					264:266	Lactobacillus paracasei TD 062	237:266	Lactobacillus paracasei TD 062	237:266	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	7	5	theme	EPS	1251:1253	arg1	content					1225:1231	the monosaccharide content	1206:1231	the monosaccharide content of the associated EPS	1206:1253	Furthermore, use of the optimized carbon source changed the monosaccharide content of the associated EPS.					
33551164	0	6	from	effect	4:9	arg1	composition					59:69	composition	59:69	composition	59:69	The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei.					
33551164	0	6	from	effect	4:9	arg1	synthesis					45:53	synthesis	45:53	synthesis	45:53	The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei.					
33551164	6	7	theme	EPS	1002:1004	arg1	content					987:993	the sugar content	977:993	the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose)	977:1129	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	7	8	theme	monosaccharide	1210:1223	arg1	content					1225:1231	the monosaccharide content	1206:1231	the monosaccharide content of the associated EPS	1206:1253	Furthermore, use of the optimized carbon source changed the monosaccharide content of the associated EPS.					
33551164	9	9	theme	carbon	1446:1451	arg1	source					1453:1458	the carbon source	1442:1458	the carbon source provided for fermentation	1442:1484	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	1	10	theme	TD	261:262	arg1	062					264:266	Lactobacillus paracasei TD 062	237:266	Lactobacillus paracasei TD 062	237:266	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	0	11	theme	exopolysaccharides	74:91	arg1	composition					59:69	composition	59:69	composition	59:69	The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei.					
33551164	0	11	theme	exopolysaccharides	74:91	arg1	synthesis					45:53	synthesis	45:53	synthesis	45:53	The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei.					
33551164	1	12	from	effect	289:294	arg1	production					325:334	production	325:334	production	325:334	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	1	12	from	effect	289:294	arg1	composition					355:365	monosaccharide composition	340:365	monosaccharide composition	340:365	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	5	13	theme	sugar	887:891	arg1	synthesis					899:907	sugar chain synthesis	887:907	sugar chain synthesis	887:907	We identified an EPS synthesis gene cluster that could participate in transport, export, and sugar chain synthesis, and generate 6 sugar nucleotides.					
33551164	9	14	theme	important	1667:1675	arg1	role					1677:1680	an important role	1664:1680	an important role for carbon source in the production of EPS by L. paracasei TD 062	1664:1746	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	0	15	theme	Lactobacillus	105:117	arg1	paracasei					119:127	Lactobacillus paracasei	105:127	Lactobacillus paracasei	105:127	The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei.					
33551164	7	16	theme	source	1191:1196	arg1	use					1163:1165	use	1163:1165	use of the optimized carbon source	1163:1196	Furthermore, use of the optimized carbon source changed the monosaccharide content of the associated EPS.					
33551164	3	17	theme	paracasei	500:508	arg1	062					513:515	L. paracasei TD 062	497:515	L. paracasei TD 062	497:515	We further investigated L. paracasei TD 062, which showed the highest EPS-producing activity (0.609 g/L), by examining the associated biosynthesis pathways for EPS.					
33551164	1	18	theme	optimal	162:168	arg1	source					177:182	the optimal carbon source	158:182	the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062	158:266	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	1	19	theme	monosaccharide	340:353	arg1	composition					355:365	monosaccharide composition	340:365	monosaccharide composition	340:365	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	9	20	theme	EPS	1721:1723	arg1	production					1707:1716	the production	1703:1716	the production of EPS by L. paracasei TD 062	1703:1746	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	3	21	theme	TD	510:511	arg1	062					513:515	L. paracasei TD 062	497:515	L. paracasei TD 062	497:515	We further investigated L. paracasei TD 062, which showed the highest EPS-producing activity (0.609 g/L), by examining the associated biosynthesis pathways for EPS.					
33551164	8	22	theme	activity	1278:1285	arg1	measurements					1287:1298	enzyme activity measurements	1271:1298	enzyme activity measurements	1271:1298	The results of enzyme activity measurements showed significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway.					
33551164	1	23	theme	carbon	170:175	arg1	source					177:182	the optimal carbon source	158:182	the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062	158:266	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	2	24	theme	strains	422:428	arg1	capacity					407:414	the EPS production capacity	388:414	the EPS production capacity of 20 strains of L. paracasei	388:444	We evaluated the EPS production capacity of 20 strains of L. paracasei under the same conditions.					
33551164	8	25	theme	significant	1307:1317	arg1	increases					1319:1327	significant increases	1307:1327	significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway	1307:1404	The results of enzyme activity measurements showed significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway.					
33551164	9	26	theme	monosaccharides	1569:1583	arg1	composition					1550:1560	the composition	1546:1560	the composition of the monosaccharides	1546:1583	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	9	27	theme	underlying	1622:1631	arg1	pathways					1643:1650	the underlying synthesis pathways	1618:1650	the underlying synthesis pathways	1618:1650	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	9	28	theme	EPS	1523:1525	arg1	production					1509:1518	the production	1505:1518	the production of EPS	1505:1525	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	3	29	theme	highest	535:541	arg1	activity					557:564	the highest EPS-producing activity	531:564	the highest EPS-producing activity (0.609 g/L)	531:576	We further investigated L. paracasei TD 062, which showed the highest EPS-producing activity (0.609 g/L), by examining the associated biosynthesis pathways for EPS.					
33551164	3	29	theme	highest	535:541	arg1	g/L					573:575	0.609 g/L	567:575	0.609 g/L	567:575	We further investigated L. paracasei TD 062, which showed the highest EPS-producing activity (0.609 g/L), by examining the associated biosynthesis pathways for EPS.					
33551164	3	30	theme	biosynthesis	607:618	arg1	pathways					620:627	the associated biosynthesis pathways	592:627	the associated biosynthesis pathways for EPS	592:635	We further investigated L. paracasei TD 062, which showed the highest EPS-producing activity (0.609 g/L), by examining the associated biosynthesis pathways for EPS.					
33551164	0	31	theme	optimized	14:22	arg1	source					31:36	optimized carbon source	14:36	optimized carbon source	14:36	The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei.					
33551164	8	32	theme	measurements	1287:1298	arg1	results					1260:1266	The results	1256:1266	The results of enzyme activity measurements	1256:1298	The results of enzyme activity measurements showed significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway.					
33551164	5	33	theme	sugar	925:929	arg1	nucleotides					931:941	6 sugar nucleotides	923:941	6 sugar nucleotides	923:941	We identified an EPS synthesis gene cluster that could participate in transport, export, and sugar chain synthesis, and generate 6 sugar nucleotides.					
33551164	2	34	theme	production	396:405	arg1	capacity					407:414	the EPS production capacity	388:414	the EPS production capacity of 20 strains of L. paracasei	388:444	We evaluated the EPS production capacity of 20 strains of L. paracasei under the same conditions.					
33551164	5	35	theme	chain	893:897	arg1	synthesis					899:907	sugar chain synthesis	887:907	sugar chain synthesis	887:907	We identified an EPS synthesis gene cluster that could participate in transport, export, and sugar chain synthesis, and generate 6 sugar nucleotides.					
33551164	3	36	theme	associated	596:605	arg1	pathways					620:627	the associated biosynthesis pathways	592:627	the associated biosynthesis pathways for EPS	592:635	We further investigated L. paracasei TD 062, which showed the highest EPS-producing activity (0.609 g/L), by examining the associated biosynthesis pathways for EPS.					
33551164	0	37	dep	synthesis	45:53	arg1	the					41:43	the	41:43	the	41:43	The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei.					
33551164	8	38	from	increases	1319:1327	arg1	activity					1336:1343	the activity	1332:1343	the activity of 2 key enzymes involved in the glycoside synthesis pathway	1332:1404	The results of enzyme activity measurements showed significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway.					
33551164	2	39	theme	EPS	392:394	arg1	capacity					407:414	the EPS production capacity	388:414	the EPS production capacity of 20 strains of L. paracasei	388:444	We evaluated the EPS production capacity of 20 strains of L. paracasei under the same conditions.					
33551164	0	40	theme	source	31:36	arg1	effect					4:9	The effect	0:9	The effect of optimized carbon source on the synthesis and composition of exopolysaccharides	0:91	The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei.					
33551164	1	41	theme	EPS	370:372	arg1	production					325:334	production	325:334	production	325:334	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	1	41	theme	EPS	370:372	arg1	composition					355:365	monosaccharide composition	340:365	monosaccharide composition	340:365	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	4	42	theme	TD	761:762	arg1	062					764:766	L. paracasei TD 062	748:766	L. paracasei TD 062	748:766	Genomics revealed that fructose, mannose, trehalose, glucose, galactose, and lactose were carbon sources that L. paracasei TD 062 could use to produce EPS.					
33551164	6	43	theme	sugar	981:985	arg1	content					987:993	the sugar content	977:993	the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose)	977:1129	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	0	44	theme	carbon	24:29	arg1	source					31:36	optimized carbon source	14:36	optimized carbon source	14:36	The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei.					
33551164	2	45	theme	paracasei	436:444	arg1	strains					422:428	20 strains	419:428	20 strains of L. paracasei	419:444	We evaluated the EPS production capacity of 20 strains of L. paracasei under the same conditions.					
33551164	8	46	theme	key	1350:1352	arg1	enzymes					1354:1360	2 key enzymes	1348:1360	2 key enzymes involved in the glycoside synthesis pathway	1348:1404	The results of enzyme activity measurements showed significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway.					
33551164	4	47	theme	paracasei	751:759	arg1	062					764:766	L. paracasei TD 062	748:766	L. paracasei TD 062	748:766	Genomics revealed that fructose, mannose, trehalose, glucose, galactose, and lactose were carbon sources that L. paracasei TD 062 could use to produce EPS.					
33551164	9	48	theme	carbon	1686:1691	arg1	source					1693:1698	carbon source	1686:1698	carbon source	1686:1698	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	1	49	theme	exopolysaccharides	209:226	arg1	production					195:204	higher production	188:204	higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062	188:266	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	9	50	theme	L.	1728:1729	arg1	062					1744:1746	L. paracasei TD 062	1728:1746	L. paracasei TD 062	1728:1746	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	3	51	theme	EPS-producing	543:555	arg1	activity					557:564	the highest EPS-producing activity	531:564	the highest EPS-producing activity (0.609 g/L)	531:576	We further investigated L. paracasei TD 062, which showed the highest EPS-producing activity (0.609 g/L), by examining the associated biosynthesis pathways for EPS.					
33551164	3	51	theme	EPS-producing	543:555	arg1	g/L					573:575	0.609 g/L	567:575	0.609 g/L	567:575	We further investigated L. paracasei TD 062, which showed the highest EPS-producing activity (0.609 g/L), by examining the associated biosynthesis pathways for EPS.					
33551164	6	52	theme	carbon	1053:1058	arg1	source					1060:1065	the optimized carbon source	1039:1065	the optimized carbon source	1039:1065	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	9	53	theme	paracasei	1731:1739	arg1	062					1744:1746	L. paracasei TD 062	1728:1746	L. paracasei TD 062	1728:1746	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	1	54	dep	production	325:334	arg1	the					321:323	the	321:323	the	321:323	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	8	55	theme	synthesis	1388:1396	arg1	pathway					1398:1404	the glycoside synthesis pathway	1374:1404	the glycoside synthesis pathway	1374:1404	The results of enzyme activity measurements showed significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway.					
33551164	6	56	dep	using	1015:1019	arg1	galactose					1107:1115	galactose	1107:1115	galactose	1107:1115	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	6	56	dep	using	1015:1019	arg1	mannose					1078:1084	mannose	1078:1084	mannose	1078:1084	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	6	56	dep	using	1015:1019	arg1	trehalose					1087:1095	trehalose	1087:1095	trehalose	1087:1095	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	6	56	dep	using	1015:1019	arg1	lactose					1122:1128	lactose	1122:1128	lactose	1122:1128	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	6	56	dep	using	1015:1019	arg1	fructose					1068:1075	fructose	1068:1075	fructose	1068:1075	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	6	56	dep	using	1015:1019	arg1	glucose					1098:1104	glucose	1098:1104	glucose	1098:1104	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	6	57	theme	optimized	1043:1051	arg1	source					1060:1065	the optimized carbon source	1039:1065	the optimized carbon source	1039:1065	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	9	58	theme	TD	1741:1742	arg1	062					1744:1746	L. paracasei TD 062	1728:1746	L. paracasei TD 062	1728:1746	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	9	59	theme	synthesis	1633:1641	arg1	pathways					1643:1650	the underlying synthesis pathways	1618:1650	the underlying synthesis pathways	1618:1650	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
33551164	8	60	theme	enzymes	1354:1360	arg1	activity					1336:1343	the activity	1332:1343	the activity of 2 key enzymes involved in the glycoside synthesis pathway	1332:1404	The results of enzyme activity measurements showed significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway.					
33551164	7	61	theme	associated	1240:1249	arg1	EPS					1251:1253	the associated EPS	1236:1253	the associated EPS	1236:1253	Furthermore, use of the optimized carbon source changed the monosaccharide content of the associated EPS.					
33551164	8	62	theme	enzyme	1271:1276	arg1	measurements					1287:1298	enzyme activity measurements	1271:1298	enzyme activity measurements	1271:1298	The results of enzyme activity measurements showed significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway.					
33551164	6	63	theme	Experimental	944:955	arg1	results					957:963	Experimental results	944:963	Experimental results	944:963	Experimental results showed that the sugar content of the EPS produced using fermentation with the optimized carbon source (fructose, mannose, trehalose, glucose, galactose, and lactose) increased by 115%.					
33551164	1	64	dep	Lactobacillus	237:249	arg1	paracasei					251:259	paracasei	251:259	paracasei	251:259	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	4	65	theme	carbon	728:733	arg1	sources					735:741	carbon sources	728:741	carbon sources that L. paracasei TD 062 could use to produce EPS	728:791	Genomics revealed that fructose, mannose, trehalose, glucose, galactose, and lactose were carbon sources that L. paracasei TD 062 could use to produce EPS.					
33551164	8	66	theme	glycoside	1378:1386	arg1	pathway					1398:1404	the glycoside synthesis pathway	1374:1404	the glycoside synthesis pathway	1374:1404	The results of enzyme activity measurements showed significant increases in the activity of 2 key enzymes involved in the glycoside synthesis pathway.					
33551164	5	67	theme	synthesis	815:823	arg1	cluster					830:836	an EPS synthesis gene cluster	808:836	an EPS synthesis gene cluster that could participate in transport, export, and sugar chain synthesis, and generate 6 sugar nucleotides	808:941	We identified an EPS synthesis gene cluster that could participate in transport, export, and sugar chain synthesis, and generate 6 sugar nucleotides.					
33551164	7	68	theme	optimized	1174:1182	arg1	source					1191:1196	the optimized carbon source	1170:1196	the optimized carbon source	1170:1196	Furthermore, use of the optimized carbon source changed the monosaccharide content of the associated EPS.					
33551164	4	69	theme	L.	748:749	arg1	062					764:766	L. paracasei TD 062	748:766	L. paracasei TD 062	748:766	Genomics revealed that fructose, mannose, trehalose, glucose, galactose, and lactose were carbon sources that L. paracasei TD 062 could use to produce EPS.					
33551164	1	70	theme	higher	188:193	arg1	production					195:204	higher production	188:204	higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062	188:266	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	5	71	theme	EPS	811:813	arg1	cluster					830:836	an EPS synthesis gene cluster	808:836	an EPS synthesis gene cluster that could participate in transport, export, and sugar chain synthesis, and generate 6 sugar nucleotides	808:941	We identified an EPS synthesis gene cluster that could participate in transport, export, and sugar chain synthesis, and generate 6 sugar nucleotides.					
33551164	7	72	theme	carbon	1184:1189	arg1	source					1191:1196	the optimized carbon source	1170:1196	the optimized carbon source	1170:1196	Furthermore, use of the optimized carbon source changed the monosaccharide content of the associated EPS.					
33551164	1	73	theme	carbon	304:309	arg1	source					311:316	this carbon source	299:316	this carbon source	299:316	This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and to evaluate the effect of this carbon source on the production and monosaccharide composition of EPS.					
33551164	3	74	theme	L.	497:498	arg1	062					513:515	L. paracasei TD 062	497:515	L. paracasei TD 062	497:515	We further investigated L. paracasei TD 062, which showed the highest EPS-producing activity (0.609 g/L), by examining the associated biosynthesis pathways for EPS.					
33551164	9	75	theme	enzyme	1599:1604	arg1	activity					1606:1613	enzyme activity	1599:1613	enzyme activity	1599:1613	Our study revealed that optimizing the carbon source provided for fermentation not only increased the production of EPS, but also affected the composition of the monosaccharides by increasing enzyme activity in the underlying synthesis pathways, suggesting an important role for carbon source in the production of EPS by L. paracasei TD 062.					
32814191	5	0	theme	differing	724:732	arg1	ratios					734:739	differing ratios	724:739	differing ratios of sugar residues	724:757	This was shown using two alginates with differing ratios of sugar residues.					
32814191	4	1	theme	free	665:668	arg1	ions					678:681	free calcium ions	665:681	free calcium ions	665:681	The adsorption of alginate was driven by dissolution of the calcite surface through complexation of free calcium ions.					
32814191	1	2	theme	selective	142:150	arg1	adsorption					152:161	selective adsorption	142:161	selective adsorption of biomolecules found in the extracellular matrix, such as polysaccharides	142:236	Bacterial anchoring to limestone rocks is thought to occur by selective adsorption of biomolecules found in the extracellular matrix, such as polysaccharides.					
32814191	6	3	dep	surface	885:891	arg1	adsorb					932:937	adsorb	932:937	to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M)	929:1032	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	6	3	dep	surface	885:891	arg1	dissolve					896:903	dissolve	896:903	to dissolve to a greater extent	893:923	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	0	4	from	effect	4:9	arg1	adsorption					38:47	adsorption	38:47	adsorption to calcium carbonate surfaces	38:77	The effect of alginate composition on adsorption to calcium carbonate surfaces.					
32814191	6	5	theme	higher	777:782	arg1	proportion					784:793	a higher proportion	775:793	a higher proportion of guluronic acid (G)	775:815	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	6	6	theme	higher	993:998	arg1	proportion					1000:1009	a higher proportion	991:1009	a higher proportion of mannuronic acid (M)	991:1032	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	8	7	theme	polymer	1265:1271	arg1	conformation					1273:1284	polymer conformation	1265:1284	polymer conformation	1265:1284	In this work, we have shown that polysaccharide adsorption to sparingly soluble calcite interfaces is closely related to polymer conformation and affinity for free calcium ions in solution.					
32814191	6	8	theme	calcium	844:850	arg1	ions					852:855	calcium ions	844:855	calcium ions	844:855	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	8	9	theme	free	1303:1306	arg1	ions					1316:1319	free calcium ions	1303:1319	free calcium ions in solution	1303:1331	In this work, we have shown that polysaccharide adsorption to sparingly soluble calcite interfaces is closely related to polymer conformation and affinity for free calcium ions in solution.					
32814191	2	10	theme	model	281:285	arg1	polysaccharide					294:307	a model matrix polysaccharide	279:307	a model matrix polysaccharide	279:307	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	2	10	theme	model	281:285	arg1	alginate					317:324	sodium alginate	310:324	sodium alginate	310:324	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	6	11	theme	greater	910:916	arg1	extent					918:923	a greater extent	908:923	a greater extent	908:923	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	8	12	theme	calcite	1224:1230	arg1	interfaces					1232:1241	sparingly soluble calcite interfaces	1206:1241	sparingly soluble calcite interfaces	1206:1241	In this work, we have shown that polysaccharide adsorption to sparingly soluble calcite interfaces is closely related to polymer conformation and affinity for free calcium ions in solution.					
32814191	2	13	theme	calcite/water	334:346	arg1	interface					348:356	the calcite/water interface	330:356	the calcite/water interface	330:356	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	6	14	contain	have	817:820	arg1	Alginates					760:768	Alginates	760:768	Alginates with a higher proportion of guluronic acid (G)	760:815	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	6	14	contain	have	817:820	arg2	affinity					831:838	a higher affinity	822:838	a higher affinity for calcium ions	822:855	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	3	15	dep	350 Å	462:466	arg1	up					456:457	up	456:457	up	456:457	Sodium alginate was found to form highly hydrated layers extending up to 350 Å into solution at concentrations up to 2.5 ppm (the inflection point of the adsorption isotherm).					
32814191	8	16	theme	soluble	1216:1222	arg1	interfaces					1232:1241	sparingly soluble calcite interfaces	1206:1241	sparingly soluble calcite interfaces	1206:1241	In this work, we have shown that polysaccharide adsorption to sparingly soluble calcite interfaces is closely related to polymer conformation and affinity for free calcium ions in solution.					
32814191	6	17	theme	higher	824:829	arg1	affinity					831:838	a higher affinity	822:838	a higher affinity for calcium ions	822:855	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	2	18	theme	adsorbed	257:264	arg1	structure					266:274	the adsorbed structure	253:274	the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface	253:356	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	1	19	theme	biomolecules	166:177	arg1	adsorption					152:161	selective adsorption	142:161	selective adsorption of biomolecules found in the extracellular matrix, such as polysaccharides	142:236	Bacterial anchoring to limestone rocks is thought to occur by selective adsorption of biomolecules found in the extracellular matrix, such as polysaccharides.					
32814191	7	20	theme	G	1064:1064	arg1	solution					1075:1082	the high G alginate solution	1055:1082	the high G alginate solution	1055:1082	Adding magnesium to the high G alginate solution reduced dissolution of the surface and the adsorbed amount.					
32814191	7	21	theme	surface	1111:1117	arg1	dissolution					1092:1102	dissolution	1092:1102	dissolution of the surface	1092:1117	Adding magnesium to the high G alginate solution reduced dissolution of the surface and the adsorbed amount.					
32814191	7	21	theme	surface	1111:1117	arg1	surface					1111:1117	the surface	1107:1117	the surface	1107:1117	Adding magnesium to the high G alginate solution reduced dissolution of the surface and the adsorbed amount.					
32814191	7	21	theme	surface	1111:1117	arg1	amount					1136:1141	the adsorbed amount	1123:1141	the adsorbed amount	1123:1141	Adding magnesium to the high G alginate solution reduced dissolution of the surface and the adsorbed amount.					
32814191	1	22	theme	Bacterial	80:88	arg1	anchoring					90:98	Bacterial anchoring	80:98	Bacterial anchoring to limestone rocks	80:117	Bacterial anchoring to limestone rocks is thought to occur by selective adsorption of biomolecules found in the extracellular matrix, such as polysaccharides.					
32814191	4	23	theme	surface	633:639	arg1	dissolution					606:616	dissolution	606:616	dissolution of the calcite surface	606:639	The adsorption of alginate was driven by dissolution of the calcite surface through complexation of free calcium ions.					
32814191	6	24	with	Alginates	760:768	arg1	proportion					784:793	a higher proportion	775:793	a higher proportion of guluronic acid (G)	775:815	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	2	25	theme	polysaccharide	294:307	arg1	structure					266:274	the adsorbed structure	253:274	the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface	253:356	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	2	26	theme	neutron	364:370	arg1	NR					384:385	NR	384:385	NR	384:385	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	2	26	theme	neutron	364:370	arg1	reflection					372:381	neutron reflection	364:381	neutron reflection (NR)	364:386	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	0	27	theme	alginate	14:21	arg1	composition					23:33	alginate composition	14:33	alginate composition	14:33	The effect of alginate composition on adsorption to calcium carbonate surfaces.					
32814191	2	28	from	interface	348:356	arg1	structure					266:274	the adsorbed structure	253:274	the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface	253:356	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	3	29	theme	inflection	519:528	arg1	point					530:534	the inflection point	515:534	the inflection point of the adsorption isotherm	515:561	Sodium alginate was found to form highly hydrated layers extending up to 350 Å into solution at concentrations up to 2.5 ppm (the inflection point of the adsorption isotherm).					
32814191	6	30	theme	acid	1025:1028	arg1	proportion					1000:1009	a higher proportion	991:1009	a higher proportion of mannuronic acid (M)	991:1032	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	6	31	with	alginates	976:984	arg1	proportion					1000:1009	a higher proportion	991:1009	a higher proportion of mannuronic acid (M)	991:1032	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	1	32	theme	limestone	103:111	arg1	rocks					113:117	limestone rocks	103:117	limestone rocks	103:117	Bacterial anchoring to limestone rocks is thought to occur by selective adsorption of biomolecules found in the extracellular matrix, such as polysaccharides.					
32814191	8	33	from	ions	1316:1319	arg1	solution					1324:1331	solution	1324:1331	solution	1324:1331	In this work, we have shown that polysaccharide adsorption to sparingly soluble calcite interfaces is closely related to polymer conformation and affinity for free calcium ions in solution.					
32814191	1	34	theme	extracellular	192:204	arg1	matrix					206:211	the extracellular matrix	188:211	the extracellular matrix	188:211	Bacterial anchoring to limestone rocks is thought to occur by selective adsorption of biomolecules found in the extracellular matrix, such as polysaccharides.					
32814191	3	35	theme	Sodium	389:394	arg1	alginate					396:403	Sodium alginate	389:403	Sodium alginate	389:403	Sodium alginate was found to form highly hydrated layers extending up to 350 Å into solution at concentrations up to 2.5 ppm (the inflection point of the adsorption isotherm).					
32814191	4	36	theme	calcium	670:676	arg1	ions					678:681	free calcium ions	665:681	free calcium ions	665:681	The adsorption of alginate was driven by dissolution of the calcite surface through complexation of free calcium ions.					
32814191	7	37	theme	alginate	1066:1073	arg1	solution					1075:1082	the high G alginate solution	1055:1082	the high G alginate solution	1055:1082	Adding magnesium to the high G alginate solution reduced dissolution of the surface and the adsorbed amount.					
32814191	4	38	theme	calcite	625:631	arg1	surface					633:639	the calcite surface	621:639	the calcite surface	621:639	The adsorption of alginate was driven by dissolution of the calcite surface through complexation of free calcium ions.					
32814191	0	39	theme	composition	23:33	arg1	effect					4:9	The effect	0:9	The effect of alginate composition on adsorption to calcium carbonate surfaces.	0:78	The effect of alginate composition on adsorption to calcium carbonate surfaces.					
32814191	5	40	theme	sugar	744:748	arg1	residues					750:757	sugar residues	744:757	sugar residues	744:757	This was shown using two alginates with differing ratios of sugar residues.					
32814191	3	41	theme	hydrated	430:437	arg1	layers					439:444	highly hydrated layers	423:444	highly hydrated layers extending up to 350 Å into solution at concentrations up to 2.5 ppm (the inflection point of the adsorption isotherm)	423:562	Sodium alginate was found to form highly hydrated layers extending up to 350 Å into solution at concentrations up to 2.5 ppm (the inflection point of the adsorption isotherm).					
32814191	5	42	theme	residues	750:757	arg1	ratios					734:739	differing ratios	724:739	differing ratios of sugar residues	724:757	This was shown using two alginates with differing ratios of sugar residues.					
32814191	1	43	located	found	179:183	arg1	matrix					206:211	the extracellular matrix	188:211	the extracellular matrix	188:211	Bacterial anchoring to limestone rocks is thought to occur by selective adsorption of biomolecules found in the extracellular matrix, such as polysaccharides.					
32814191	1	43	located	found	179:183	arg2	biomolecules					166:177	biomolecules	166:177	biomolecules	166:177	Bacterial anchoring to limestone rocks is thought to occur by selective adsorption of biomolecules found in the extracellular matrix, such as polysaccharides.					
32814191	1	43	located	found	179:183	arg2	polysaccharides					222:236	polysaccharides	222:236	polysaccharides	222:236	Bacterial anchoring to limestone rocks is thought to occur by selective adsorption of biomolecules found in the extracellular matrix, such as polysaccharides.					
32814191	3	44	theme	adsorption	543:552	arg1	isotherm					554:561	the adsorption isotherm	539:561	the adsorption isotherm	539:561	Sodium alginate was found to form highly hydrated layers extending up to 350 Å into solution at concentrations up to 2.5 ppm (the inflection point of the adsorption isotherm).					
32814191	4	45	theme	ions	678:681	arg1	complexation					649:660	complexation	649:660	complexation of free calcium ions	649:681	The adsorption of alginate was driven by dissolution of the calcite surface through complexation of free calcium ions.					
32814191	6	46	theme	acid	808:811	arg1	proportion					784:793	a higher proportion	775:793	a higher proportion of guluronic acid (G)	775:815	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	3	47	theme	isotherm	554:561	arg1	point					530:534	the inflection point	515:534	the inflection point of the adsorption isotherm	515:561	Sodium alginate was found to form highly hydrated layers extending up to 350 Å into solution at concentrations up to 2.5 ppm (the inflection point of the adsorption isotherm).					
32814191	3	48	dep	2.5 ppm	506:512	arg1	point					530:534	the inflection point	515:534	the inflection point of the adsorption isotherm	515:561	Sodium alginate was found to form highly hydrated layers extending up to 350 Å into solution at concentrations up to 2.5 ppm (the inflection point of the adsorption isotherm).					
32814191	3	48	dep	2.5 ppm	506:512	arg1	up					500:501	up	500:501	up	500:501	Sodium alginate was found to form highly hydrated layers extending up to 350 Å into solution at concentrations up to 2.5 ppm (the inflection point of the adsorption isotherm).					
32814191	6	49	theme	guluronic	798:806	arg1	G					814:814	G	814:814	G	814:814	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	6	49	theme	guluronic	798:806	arg1	acid					808:811	guluronic acid	798:811	guluronic acid (G)	798:815	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	8	50	theme	polysaccharide	1177:1190	arg1	adsorption					1192:1201	polysaccharide adsorption	1177:1201	polysaccharide adsorption to sparingly soluble calcite interfaces	1177:1241	In this work, we have shown that polysaccharide adsorption to sparingly soluble calcite interfaces is closely related to polymer conformation and affinity for free calcium ions in solution.					
32814191	2	51	theme	matrix	287:292	arg1	polysaccharide					294:307	a model matrix polysaccharide	279:307	a model matrix polysaccharide	279:307	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	2	51	theme	matrix	287:292	arg1	alginate					317:324	sodium alginate	310:324	sodium alginate	310:324	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	8	52	theme	calcium	1308:1314	arg1	ions					1316:1319	free calcium ions	1303:1319	free calcium ions in solution	1303:1331	In this work, we have shown that polysaccharide adsorption to sparingly soluble calcite interfaces is closely related to polymer conformation and affinity for free calcium ions in solution.					
32814191	2	53	theme	sodium	310:315	arg1	polysaccharide					294:307	a model matrix polysaccharide	279:307	a model matrix polysaccharide	279:307	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	2	53	theme	sodium	310:315	arg1	alginate					317:324	sodium alginate	310:324	sodium alginate	310:324	Here we study the adsorbed structure of a model matrix polysaccharide, sodium alginate, at the calcite/water interface using neutron reflection (NR).					
32814191	0	54	theme	carbonate	60:68	arg1	surfaces					70:77	calcium carbonate surfaces	52:77	calcium carbonate surfaces	52:77	The effect of alginate composition on adsorption to calcium carbonate surfaces.					
32814191	5	55	with	alginates	709:717	arg1	ratios					734:739	differing ratios	724:739	differing ratios of sugar residues	724:757	This was shown using two alginates with differing ratios of sugar residues.					
32814191	6	56	theme	mannuronic	1014:1023	arg1	M					1031:1031	M	1031:1031	M	1031:1031	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	6	56	theme	mannuronic	1014:1023	arg1	acid					1025:1028	mannuronic acid	1014:1028	mannuronic acid (M)	1014:1032	Alginates with a higher proportion of guluronic acid (G) have a higher affinity for calcium ions and were found to cause the surface to dissolve to a greater extent and to adsorb more at the surface when compared to alginates with a higher proportion of mannuronic acid (M).					
32814191	4	57	theme	alginate	583:590	arg1	adsorption					569:578	The adsorption	565:578	The adsorption of alginate	565:590	The adsorption of alginate was driven by dissolution of the calcite surface through complexation of free calcium ions.					
32814191	0	58	theme	calcium	52:58	arg1	surfaces					70:77	calcium carbonate surfaces	52:77	calcium carbonate surfaces	52:77	The effect of alginate composition on adsorption to calcium carbonate surfaces.					
32814191	7	59	theme	high	1059:1062	arg1	solution					1075:1082	the high G alginate solution	1055:1082	the high G alginate solution	1055:1082	Adding magnesium to the high G alginate solution reduced dissolution of the surface and the adsorbed amount.					
32814191	7	60	theme	adsorbed	1127:1134	arg1	surface					1111:1117	the surface	1107:1117	the surface	1107:1117	Adding magnesium to the high G alginate solution reduced dissolution of the surface and the adsorbed amount.					
32814191	7	60	theme	adsorbed	1127:1134	arg1	amount					1136:1141	the adsorbed amount	1123:1141	the adsorbed amount	1123:1141	Adding magnesium to the high G alginate solution reduced dissolution of the surface and the adsorbed amount.					
33556214	2	0	theme	yield	607:611	arg1	increase					613:620	250 h granted highest yield increase	585:620	250 h granted highest yield increase	585:620	METHODS AND RESULTS Supplementation at 5 g l-1 combined with cultivation time of 250 h granted highest yield increase in mycelia (by 122%).					
33556214	7	1	with	stream	1095:1100	arg1	content					1116:1122	high oil content	1107:1122	high oil content	1107:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	3	2	theme	extraction	672:681	arg1	yield					683:687	extraction yield	672:687	extraction yield	672:687	The supplementation reduced extraction yield and decreased the molecular weight of the main IPS population.					
33556214	2	3	theme	granted	591:597	arg1	increase					613:620	250 h granted highest yield increase	585:620	250 h granted highest yield increase	585:620	METHODS AND RESULTS Supplementation at 5 g l-1 combined with cultivation time of 250 h granted highest yield increase in mycelia (by 122%).					
33556214	7	4	theme	side	1090:1093	arg1	stream					1095:1100	a food industry fibrous side stream	1066:1100	a food industry fibrous side stream with high oil content	1066:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	7	4	theme	side	1090:1093	arg1	cake					1058:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	10	5	theme	buckthorn	1381:1389	arg1	cake					1397:1400	Sea buckthorn press cake	1377:1400	Sea buckthorn press cake	1377:1400	Sea buckthorn press cake is a promising supplement for increasing mycelium production.					
33556214	10	5	theme	buckthorn	1381:1389	arg1	supplement					1417:1426	a promising supplement	1405:1426	a promising supplement for increasing mycelium production	1405:1461	Sea buckthorn press cake is a promising supplement for increasing mycelium production.					
33556214	1	6	theme	mycelium	259:266	arg1	yield					268:272	mycelium yield	259:272	mycelium yield of Inonotus obliquus in submerged cultivation	259:318	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	2	7	dep	combined	551:558	arg1	%					640:640	122%	637:640	122%	637:640	METHODS AND RESULTS Supplementation at 5 g l-1 combined with cultivation time of 250 h granted highest yield increase in mycelia (by 122%).					
33556214	7	8	theme	industry	1073:1080	arg1	stream					1095:1100	a food industry fibrous side stream	1066:1100	a food industry fibrous side stream with high oil content	1066:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	7	8	theme	industry	1073:1080	arg1	cake					1058:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	1	9	theme	intracellular	444:456	arg1	polysaccharides					458:472	intracellular polysaccharides	444:472	intracellular polysaccharides (IPS)	444:478	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	9	theme	intracellular	444:456	arg1	IPS					475:477	IPS	475:477	IPS	475:477	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	5	10	theme	rhamnose	860:867	arg1	content					835:841	The relative content	822:841	The relative content of arabinose and rhamnose in EPS	822:874	The relative content of arabinose and rhamnose in EPS positively correlated with dosage of the press cake.					
33556214	3	11	theme	IPS	736:738	arg1	population					740:749	the main IPS population	727:749	the main IPS population	727:749	The supplementation reduced extraction yield and decreased the molecular weight of the main IPS population.					
33556214	1	12	theme	time	192:195	arg1	influence					167:175	the influence	163:175	the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS)	163:478	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	8	13	theme	obliquus	1172:1179	arg1	mycelium					1181:1188	Inonotus obliquus mycelium	1163:1188	Inonotus obliquus mycelium	1163:1188	It increases the cultivation yield of Inonotus obliquus mycelium and influences the produced polysaccharides.					
33556214	1	14	theme	macromolecular	363:376	arg1	properties					378:387	macromolecular properties	363:387	macromolecular properties	363:387	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	15	theme	sea	201:203	arg1	cake					221:224	sea buckthorn press cake	201:224	sea buckthorn press cake (Hippophaë rhamnoides)	201:247	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	15	theme	sea	201:203	arg1	rhamnoides					237:246	Hippophaë rhamnoides	227:246	Hippophaë rhamnoides	227:246	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	0	16	from	growth	67:72	arg1	cultivation					128:138	submerged cultivation	118:138	submerged cultivation	118:138	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	7	17	theme	buckthorn	1042:1050	arg1	stream					1095:1100	a food industry fibrous side stream	1066:1100	a food industry fibrous side stream with high oil content	1066:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	7	17	theme	buckthorn	1042:1050	arg1	cake					1058:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	5	18	theme	arabinose	846:854	arg1	content					835:841	The relative content	822:841	The relative content of arabinose and rhamnose in EPS	822:874	The relative content of arabinose and rhamnose in EPS positively correlated with dosage of the press cake.					
33556214	6	19	theme	press	933:937	arg1	supplementation					944:958	The press cake supplementation	929:958	The press cake supplementation	929:958	The press cake supplementation increased the content of galacturonic acid in IPS, but not in EPS.					
33556214	7	20	theme	CONCLUSION	1027:1036	arg1	stream					1095:1100	a food industry fibrous side stream	1066:1100	a food industry fibrous side stream with high oil content	1066:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	7	20	theme	CONCLUSION	1027:1036	arg1	cake					1058:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	1	21	theme	exopolysaccharides	396:413	arg1	yield					331:335	the yield	327:335	the yield	327:335	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	21	theme	exopolysaccharides	396:413	arg1	composition					346:356	monomer composition	338:356	monomer composition	338:356	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	21	theme	exopolysaccharides	396:413	arg1	properties					378:387	macromolecular properties	363:387	macromolecular properties	363:387	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	2	22	theme	cultivation	565:575	arg1	time					577:580	cultivation time	565:580	cultivation time of 250 h granted highest yield increase	565:620	METHODS AND RESULTS Supplementation at 5 g l-1 combined with cultivation time of 250 h granted highest yield increase in mycelia (by 122%).					
33556214	10	23	theme	press	1391:1395	arg1	cake					1397:1400	Sea buckthorn press cake	1377:1400	Sea buckthorn press cake	1377:1400	Sea buckthorn press cake is a promising supplement for increasing mycelium production.					
33556214	10	23	theme	press	1391:1395	arg1	supplement					1417:1426	a promising supplement	1405:1426	a promising supplement for increasing mycelium production	1405:1461	Sea buckthorn press cake is a promising supplement for increasing mycelium production.					
33556214	11	24	theme	stream	1506:1511	arg1	utilization					1468:1478	The utilization	1464:1478	The utilization of this agricultural side stream	1464:1511	The utilization of this agricultural side stream would therefore favour circular economy.					
33556214	5	25	theme	cake	923:926	arg1	dosage					903:908	dosage	903:908	dosage of the press cake	903:926	The relative content of arabinose and rhamnose in EPS positively correlated with dosage of the press cake.					
33556214	0	26	from	polysaccharides	78:92	arg1	cultivation					128:138	submerged cultivation	118:138	submerged cultivation	118:138	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	11	27	theme	agricultural	1488:1499	arg1	stream					1506:1511	this agricultural side stream	1483:1511	this agricultural side stream	1483:1511	The utilization of this agricultural side stream would therefore favour circular economy.					
33556214	9	28	theme	STUDY	1266:1270	arg1	Mycelium					1272:1279	THE STUDY Mycelium	1262:1279	THE STUDY Mycelium	1262:1279	SIGNIFICANCE AND IMPACT OF THE STUDY Mycelium is a resource of bioactive polysaccharides, attracting the interest of nutraceutical companies.					
33556214	1	29	from	composition	346:356	arg1	Investigation					146:158	AIMS Investigation	141:158	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS)	141:478	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	30	from	influence	167:175	arg1	yield					331:335	the yield	327:335	the yield	327:335	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	30	from	influence	167:175	arg1	composition					346:356	monomer composition	338:356	monomer composition	338:356	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	30	from	influence	167:175	arg1	yield					268:272	mycelium yield	259:272	mycelium yield of Inonotus obliquus in submerged cultivation	259:318	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	30	from	influence	167:175	arg1	properties					378:387	macromolecular properties	363:387	macromolecular properties	363:387	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	11	31	theme	circular	1536:1543	arg1	economy					1545:1551	circular economy	1536:1551	circular economy	1536:1551	The utilization of this agricultural side stream would therefore favour circular economy.					
33556214	6	32	theme	acid	998:1001	arg1	content					974:980	the content	970:980	the content of galacturonic acid	970:1001	The press cake supplementation increased the content of galacturonic acid in IPS, but not in EPS.					
33556214	0	33	from	Effects	0:6	arg1	growth					67:72	mycelium growth	58:72	mycelium growth	58:72	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	0	33	from	Effects	0:6	arg1	polysaccharides					78:92	polysaccharides	78:92	polysaccharides	78:92	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	1	34	theme	monomer	338:344	arg1	composition					346:356	monomer composition	338:356	monomer composition	338:356	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	0	35	theme	Inonotus	97:104	arg1	obliquus					106:113	Inonotus obliquus	97:113	Inonotus obliquus	97:113	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	9	36	theme	bioactive	1298:1306	arg1	polysaccharides					1308:1322	bioactive polysaccharides	1298:1322	bioactive polysaccharides	1298:1322	SIGNIFICANCE AND IMPACT OF THE STUDY Mycelium is a resource of bioactive polysaccharides, attracting the interest of nutraceutical companies.					
33556214	0	37	theme	buckthorn	34:42	arg1	cake					50:53	sea buckthorn press cake	30:53	sea buckthorn press cake	30:53	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	8	38	theme	cultivation	1142:1152	arg1	yield					1154:1158	the cultivation yield	1138:1158	the cultivation yield of Inonotus obliquus mycelium	1138:1188	It increases the cultivation yield of Inonotus obliquus mycelium and influences the produced polysaccharides.					
33556214	0	39	theme	supplementation	11:25	arg1	Effects					0:6	Effects	0:6	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.	0:139	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	1	40	theme	Inonotus	277:284	arg1	obliquus					286:293	Inonotus obliquus	277:293	Inonotus obliquus	277:293	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	4	41	theme	EPS	817:819	arg1	production					782:791	production	782:791	production	782:791	The supplementation increased production and molecular weight of EPS.					
33556214	4	41	theme	EPS	817:819	arg1	weight					807:812	molecular weight	797:812	molecular weight	797:812	The supplementation increased production and molecular weight of EPS.					
33556214	0	42	theme	sea	30:32	arg1	cake					50:53	sea buckthorn press cake	30:53	sea buckthorn press cake	30:53	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	0	43	theme	press	44:48	arg1	cake					50:53	sea buckthorn press cake	30:53	sea buckthorn press cake	30:53	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	9	44	theme	companies	1366:1374	arg1	interest					1340:1347	the interest	1336:1347	the interest of nutraceutical companies	1336:1374	SIGNIFICANCE AND IMPACT OF THE STUDY Mycelium is a resource of bioactive polysaccharides, attracting the interest of nutraceutical companies.					
33556214	1	45	theme	cake	221:224	arg1	influence					167:175	the influence	163:175	the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS)	163:478	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	46	from	polysaccharides	458:472	arg1	yield					331:335	the yield	327:335	the yield	327:335	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	46	from	polysaccharides	458:472	arg1	composition					346:356	monomer composition	338:356	monomer composition	338:356	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	46	from	polysaccharides	458:472	arg1	properties					378:387	macromolecular properties	363:387	macromolecular properties	363:387	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	47	theme	Hippophaë	227:235	arg1	cake					221:224	sea buckthorn press cake	201:224	sea buckthorn press cake (Hippophaë rhamnoides)	201:247	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	47	theme	Hippophaë	227:235	arg1	rhamnoides					237:246	Hippophaë rhamnoides	227:246	Hippophaë rhamnoides	227:246	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	7	48	theme	high	1107:1110	arg1	content					1116:1122	high oil content	1107:1122	high oil content	1107:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	1	49	theme	submerged	298:306	arg1	cultivation					308:318	submerged cultivation	298:318	submerged cultivation	298:318	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	2	50	theme	highest	599:605	arg1	increase					613:620	250 h granted highest yield increase	585:620	250 h granted highest yield increase	585:620	METHODS AND RESULTS Supplementation at 5 g l-1 combined with cultivation time of 250 h granted highest yield increase in mycelia (by 122%).					
33556214	7	51	theme	fibrous	1082:1088	arg1	stream					1095:1100	a food industry fibrous side stream	1066:1100	a food industry fibrous side stream with high oil content	1066:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	7	51	theme	fibrous	1082:1088	arg1	cake					1058:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	1	52	theme	culture	426:432	arg1	media					434:438	culture media	426:438	culture media	426:438	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	7	53	theme	food	1068:1071	arg1	stream					1095:1100	a food industry fibrous side stream	1066:1100	a food industry fibrous side stream with high oil content	1066:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	7	53	theme	food	1068:1071	arg1	cake					1058:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	10	54	theme	Sea	1377:1379	arg1	cake					1397:1400	Sea buckthorn press cake	1377:1400	Sea buckthorn press cake	1377:1400	Sea buckthorn press cake is a promising supplement for increasing mycelium production.					
33556214	10	54	theme	Sea	1377:1379	arg1	supplement					1417:1426	a promising supplement	1405:1426	a promising supplement for increasing mycelium production	1405:1461	Sea buckthorn press cake is a promising supplement for increasing mycelium production.					
33556214	1	55	theme	influence	167:175	arg1	Investigation					146:158	AIMS Investigation	141:158	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS)	141:478	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	7	56	theme	oil	1112:1114	arg1	content					1116:1122	high oil content	1107:1122	high oil content	1107:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	8	57	theme	produced	1209:1216	arg1	polysaccharides					1218:1232	the produced polysaccharides	1205:1232	the produced polysaccharides	1205:1232	It increases the cultivation yield of Inonotus obliquus mycelium and influences the produced polysaccharides.					
33556214	3	58	theme	molecular	707:715	arg1	weight					717:722	the molecular weight	703:722	the molecular weight of the main IPS population	703:749	The supplementation reduced extraction yield and decreased the molecular weight of the main IPS population.					
33556214	1	59	from	yield	268:272	arg1	Investigation					146:158	AIMS Investigation	141:158	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS)	141:478	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	59	from	yield	268:272	arg1	cultivation					308:318	submerged cultivation	298:318	submerged cultivation	298:318	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	10	60	theme	promising	1407:1415	arg1	cake					1397:1400	Sea buckthorn press cake	1377:1400	Sea buckthorn press cake	1377:1400	Sea buckthorn press cake is a promising supplement for increasing mycelium production.					
33556214	10	60	theme	promising	1407:1415	arg1	supplement					1417:1426	a promising supplement	1405:1426	a promising supplement for increasing mycelium production	1405:1461	Sea buckthorn press cake is a promising supplement for increasing mycelium production.					
33556214	2	61	from	5 g l-1	543:549	arg1	Supplementation					524:538	Supplementation	524:538	Supplementation at 5 g l-1	524:549	METHODS AND RESULTS Supplementation at 5 g l-1 combined with cultivation time of 250 h granted highest yield increase in mycelia (by 122%).					
33556214	1	62	theme	cultivation	180:190	arg1	time					192:195	cultivation time	180:195	cultivation time	180:195	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	2	63	theme	increase	613:620	arg1	time					577:580	cultivation time	565:580	cultivation time of 250 h granted highest yield increase	565:620	METHODS AND RESULTS Supplementation at 5 g l-1 combined with cultivation time of 250 h granted highest yield increase in mycelia (by 122%).					
33556214	3	64	theme	population	740:749	arg1	weight					717:722	the molecular weight	703:722	the molecular weight of the main IPS population	703:749	The supplementation reduced extraction yield and decreased the molecular weight of the main IPS population.					
33556214	7	65	theme	press	1052:1056	arg1	stream					1095:1100	a food industry fibrous side stream	1066:1100	a food industry fibrous side stream with high oil content	1066:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	7	65	theme	press	1052:1056	arg1	cake					1058:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	5	66	theme	relative	826:833	arg1	content					835:841	The relative content	822:841	The relative content of arabinose and rhamnose in EPS	822:874	The relative content of arabinose and rhamnose in EPS positively correlated with dosage of the press cake.					
33556214	8	67	theme	Inonotus	1163:1170	arg1	mycelium					1181:1188	Inonotus obliquus mycelium	1163:1188	Inonotus obliquus mycelium	1163:1188	It increases the cultivation yield of Inonotus obliquus mycelium and influences the produced polysaccharides.					
33556214	3	68	theme	main	731:734	arg1	population					740:749	the main IPS population	727:749	the main IPS population	727:749	The supplementation reduced extraction yield and decreased the molecular weight of the main IPS population.					
33556214	7	69	theme	Sea	1038:1040	arg1	stream					1095:1100	a food industry fibrous side stream	1066:1100	a food industry fibrous side stream with high oil content	1066:1122	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	7	69	theme	Sea	1038:1040	arg1	cake					1058:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake	1027:1061	CONCLUSION Sea buckthorn press cake is a food industry fibrous side stream with high oil content.					
33556214	1	70	theme	buckthorn	205:213	arg1	cake					221:224	sea buckthorn press cake	201:224	sea buckthorn press cake (Hippophaë rhamnoides)	201:247	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	70	theme	buckthorn	205:213	arg1	rhamnoides					237:246	Hippophaë rhamnoides	227:246	Hippophaë rhamnoides	227:246	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	8	71	theme	mycelium	1181:1188	arg1	yield					1154:1158	the cultivation yield	1138:1158	the cultivation yield of Inonotus obliquus mycelium	1138:1188	It increases the cultivation yield of Inonotus obliquus mycelium and influences the produced polysaccharides.					
33556214	6	72	theme	cake	939:942	arg1	supplementation					944:958	The press cake supplementation	929:958	The press cake supplementation	929:958	The press cake supplementation increased the content of galacturonic acid in IPS, but not in EPS.					
33556214	0	73	theme	submerged	118:126	arg1	cultivation					128:138	submerged cultivation	118:138	submerged cultivation	118:138	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	11	74	theme	side	1501:1504	arg1	stream					1506:1511	this agricultural side stream	1483:1511	this agricultural side stream	1483:1511	The utilization of this agricultural side stream would therefore favour circular economy.					
33556214	5	75	from	content	835:841	arg1	EPS					872:874	EPS	872:874	EPS	872:874	The relative content of arabinose and rhamnose in EPS positively correlated with dosage of the press cake.					
33556214	5	76	theme	press	917:921	arg1	cake					923:926	the press cake	913:926	the press cake	913:926	The relative content of arabinose and rhamnose in EPS positively correlated with dosage of the press cake.					
33556214	0	77	theme	mycelium	58:65	arg1	growth					67:72	mycelium growth	58:72	mycelium growth	58:72	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	1	78	theme	AIMS	141:144	arg1	Investigation					146:158	AIMS Investigation	141:158	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS)	141:478	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	9	79	theme	Mycelium	1272:1279	arg1	IMPACT					1252:1257	IMPACT	1252:1257	IMPACT	1252:1257	SIGNIFICANCE AND IMPACT OF THE STUDY Mycelium is a resource of bioactive polysaccharides, attracting the interest of nutraceutical companies.					
33556214	9	79	theme	Mycelium	1272:1279	arg1	SIGNIFICANCE					1235:1246	SIGNIFICANCE	1235:1246	SIGNIFICANCE	1235:1246	SIGNIFICANCE AND IMPACT OF THE STUDY Mycelium is a resource of bioactive polysaccharides, attracting the interest of nutraceutical companies.					
33556214	9	79	theme	Mycelium	1272:1279	arg1	resource					1286:1293	a resource	1284:1293	a resource of bioactive polysaccharides	1284:1322	SIGNIFICANCE AND IMPACT OF THE STUDY Mycelium is a resource of bioactive polysaccharides, attracting the interest of nutraceutical companies.					
33556214	10	80	theme	mycelium	1443:1450	arg1	production					1452:1461	mycelium production	1443:1461	mycelium production	1443:1461	Sea buckthorn press cake is a promising supplement for increasing mycelium production.					
33556214	6	81	theme	galacturonic	985:996	arg1	acid					998:1001	galacturonic acid	985:1001	galacturonic acid	985:1001	The press cake supplementation increased the content of galacturonic acid in IPS, but not in EPS.					
33556214	0	82	theme	obliquus	106:113	arg1	growth					67:72	mycelium growth	58:72	mycelium growth	58:72	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	0	82	theme	obliquus	106:113	arg1	polysaccharides					78:92	polysaccharides	78:92	polysaccharides	78:92	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	1	83	from	Investigation	146:158	arg1	yield					331:335	the yield	327:335	the yield	327:335	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	83	from	Investigation	146:158	arg1	composition					346:356	monomer composition	338:356	monomer composition	338:356	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	83	from	Investigation	146:158	arg1	yield					268:272	mycelium yield	259:272	mycelium yield of Inonotus obliquus in submerged cultivation	259:318	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	83	from	Investigation	146:158	arg1	properties					378:387	macromolecular properties	363:387	macromolecular properties	363:387	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	9	84	theme	polysaccharides	1308:1322	arg1	IMPACT					1252:1257	IMPACT	1252:1257	IMPACT	1252:1257	SIGNIFICANCE AND IMPACT OF THE STUDY Mycelium is a resource of bioactive polysaccharides, attracting the interest of nutraceutical companies.					
33556214	9	84	theme	polysaccharides	1308:1322	arg1	SIGNIFICANCE					1235:1246	SIGNIFICANCE	1235:1246	SIGNIFICANCE	1235:1246	SIGNIFICANCE AND IMPACT OF THE STUDY Mycelium is a resource of bioactive polysaccharides, attracting the interest of nutraceutical companies.					
33556214	9	84	theme	polysaccharides	1308:1322	arg1	resource					1286:1293	a resource	1284:1293	a resource of bioactive polysaccharides	1284:1322	SIGNIFICANCE AND IMPACT OF THE STUDY Mycelium is a resource of bioactive polysaccharides, attracting the interest of nutraceutical companies.					
33556214	2	85	dep	METHODS	504:510	arg1	combined					551:558	combined	551:558	combined with cultivation time of 250 h granted highest yield increase in mycelia (by 122%)	551:641	METHODS AND RESULTS Supplementation at 5 g l-1 combined with cultivation time of 250 h granted highest yield increase in mycelia (by 122%).					
33556214	1	86	from	yield	331:335	arg1	Investigation					146:158	AIMS Investigation	141:158	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS)	141:478	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	87	theme	obliquus	286:293	arg1	yield					268:272	mycelium yield	259:272	mycelium yield of Inonotus obliquus in submerged cultivation	259:318	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	88	dep	time	192:195	arg1	dosage					249:254	dosage	249:254	dosage	249:254	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	89	from	properties	378:387	arg1	Investigation					146:158	AIMS Investigation	141:158	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS)	141:478	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	0	90	theme	cake	50:53	arg1	supplementation					11:25	supplementation	11:25	supplementation of sea buckthorn press cake	11:53	Effects of supplementation of sea buckthorn press cake on mycelium growth and polysaccharides of Inonotus obliquus in submerged cultivation.					
33556214	9	91	theme	nutraceutical	1352:1364	arg1	companies					1366:1374	nutraceutical companies	1352:1374	nutraceutical companies	1352:1374	SIGNIFICANCE AND IMPACT OF THE STUDY Mycelium is a resource of bioactive polysaccharides, attracting the interest of nutraceutical companies.					
33556214	1	92	theme	press	215:219	arg1	cake					221:224	sea buckthorn press cake	201:224	sea buckthorn press cake (Hippophaë rhamnoides)	201:247	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	92	theme	press	215:219	arg1	rhamnoides					237:246	Hippophaë rhamnoides	227:246	Hippophaë rhamnoides	227:246	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	93	from	media	434:438	arg1	yield					331:335	the yield	327:335	the yield	327:335	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	93	from	media	434:438	arg1	composition					346:356	monomer composition	338:356	monomer composition	338:356	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	1	93	from	media	434:438	arg1	properties					378:387	macromolecular properties	363:387	macromolecular properties	363:387	AIMS Investigation of the influence of cultivation time and sea buckthorn press cake (Hippophaë rhamnoides) dosage on mycelium yield of Inonotus obliquus in submerged cultivation and on the yield, monomer composition, and macromolecular properties of the exopolysaccharides (EPS) from culture media and intracellular polysaccharides (IPS) extracted from mycelia.					
33556214	4	94	theme	molecular	797:805	arg1	weight					807:812	molecular weight	797:812	molecular weight	797:812	The supplementation increased production and molecular weight of EPS.					
32538719	22	0	theme	mRNA	2178:2181	arg1	expression					2183:2192	lower mRNA expression	2172:2192	lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice	2172:2314	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	5	1	theme	cytokines	414:422	arg1	production					394:403	the production	390:403	the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues	390:516	In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.					
32538719	7	2	theme	prime	742:746	arg1	kit					781:783	prime script 1st strand cDNA synthesis kit	742:783	prime script 1st strand cDNA synthesis kit	742:783	The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit.					
32538719	12	3	dep	increased	1112:1120	arg1	Ruminococcaceae					1156:1170	Ruminococcaceae	1156:1170	Ruminococcaceae	1156:1170	increased beneficial intestinal microflora (Ruminococcaceae and Akkermansia muciniphila) and decreased the community of harmful microflora (Enterobacteriaceae, including Escherichia coli and Bacteroides sp.)					
32538719	12	3	dep	increased	1112:1120	arg1	muciniphila					1188:1198	muciniphila	1188:1198	muciniphila	1188:1198	increased beneficial intestinal microflora (Ruminococcaceae and Akkermansia muciniphila) and decreased the community of harmful microflora (Enterobacteriaceae, including Escherichia coli and Bacteroides sp.)					
32538719	22	4	theme	harmful	2365:2371	arg1	microflora					2379:2388	harmful fecal microflora	2365:2388	harmful fecal microflora	2365:2388	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	14	5	theme	mesenteric	1462:1471	arg1	node					1479:1482	mesenteric lymph node	1462:1482	mesenteric lymph node	1462:1482	Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05).					
32538719	5	6	theme	fatty	476:480	arg1	composition					487:497	fatty acid composition	476:497	fatty acid composition	476:497	In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.					
32538719	3	7	theme	health	247:252	arg1	function					254:261	optimal gut health function	235:261	optimal gut health function	235:261	has been associated with relieving inflammation and maintaining optimal gut health function.					
32538719	5	8	theme	major	408:412	arg1	cytokines					414:422	major cytokines	408:422	major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues	408:516	In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.					
32538719	20	9	dep	CONCLUSION	2002:2011	arg1	showed					2033:2038	showed	2033:2038	showed that supplementation of mice with S. sieboldii MIQ	2033:2089	CONCLUSION The present results showed that supplementation of mice with S. sieboldii MIQ.					
32538719	22	10	theme	IL-10	2260:2264	arg1	expression					2183:2192	lower mRNA expression	2172:2192	lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice	2172:2314	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	3	11	theme	optimal	235:241	arg1	function					254:261	optimal gut health function	235:261	optimal gut health function	235:261	has been associated with relieving inflammation and maintaining optimal gut health function.					
32538719	16	12	theme	control	1660:1666	arg1	P<0.05					1675:1680	P<0.05	1675:1680	P<0.05	1675:1680	group showed a higher percentage of 6:0 and 18:2n-6 compared with that in the control group (P<0.05).					
32538719	16	12	theme	control	1660:1666	arg1	group					1668:1672	the control group	1656:1672	the control group (P<0.05)	1656:1681	group showed a higher percentage of 6:0 and 18:2n-6 compared with that in the control group (P<0.05).					
32538719	5	13	theme	related	448:454	arg1	cytokines					414:422	major cytokines	408:422	major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues	408:516	In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.					
32538719	20	14	theme	sieboldii	2077:2085	arg1	MIQ					2087:2089	S. sieboldii MIQ	2074:2089	S. sieboldii MIQ	2074:2089	CONCLUSION The present results showed that supplementation of mice with S. sieboldii MIQ.					
32538719	22	15	theme	anti-inflammatory	2233:2249	arg1	IL-10					2260:2264	anti-inflammatory cytokine IL-10	2233:2264	anti-inflammatory cytokine IL-10	2233:2264	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	14	16	from	expression	1420:1429	arg1	node					1479:1482	mesenteric lymph node	1462:1482	mesenteric lymph node	1462:1482	Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05).					
32538719	6	17	theme	profiling	597:605	arg1	analysis					607:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	MATERIALS AND METHODS 16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed using EzBioCloud data base.					
32538719	19	18	theme	monounsaturated	1917:1931	arg1	acids					1968:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids	1900:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues	1900:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	14	19	theme	mRNA	1415:1418	arg1	expression					1420:1429	a significantly lower mRNA expression	1393:1429	a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node	1393:1482	Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05).					
32538719	6	20	theme	microbiome	576:585	arg1	analysis					607:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	MATERIALS AND METHODS 16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed using EzBioCloud data base.					
32538719	11	21	theme	S.	1094:1095	arg1	MIQ					1107:1109	S. sieboldii MIQ	1094:1109	S. sieboldii MIQ	1094:1109	16S rDNA sequencing analysis of fecal samples showed that supplementation with S. sieboldii MIQ.					
32538719	22	22	theme	cytokine	2214:2221	arg1	IL-6					2223:2226	pro-inflammatory cytokine IL-6	2197:2226	pro-inflammatory cytokine IL-6	2197:2226	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	6	23	theme	DNA	555:557	arg1	analysis					607:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	MATERIALS AND METHODS 16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed using EzBioCloud data base.					
32538719	7	24	theme	mesenteric	681:690	arg1	node					698:701	the mesenteric lymph node	677:701	the mesenteric lymph node	677:701	The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit.					
32538719	11	25	theme	rDNA	1019:1022	arg1	sequencing					1024:1033	16S rDNA sequencing	1015:1033	16S rDNA sequencing analysis of fecal samples	1015:1059	16S rDNA sequencing analysis of fecal samples showed that supplementation with S. sieboldii MIQ.					
32538719	8	26	theme	cDNA	830:833	arg1	samples					835:841	cDNA samples	830:841	cDNA samples using the SYBR™ Green PCR Master Mix	830:878	Quantitative real-time PCR was performed on cDNA samples using the SYBR™ Green PCR Master Mix.					
32538719	19	27	located	found	1974:1978	arg1	tissues					1993:1999	tissues	1993:1999	tissues	1993:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	19	27	located	found	1974:1978	arg2	acids					1968:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids	1900:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues	1900:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	19	27	located	found	1974:1978	arg1	feces					1983:1987	feces	1983:1987	feces	1983:1987	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	5	28	theme	several	502:508	arg1	tissues					510:516	several tissues	502:516	several tissues	502:516	In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.					
32538719	14	29	theme	lower	1409:1413	arg1	expression					1420:1429	a significantly lower mRNA expression	1393:1429	a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node	1393:1482	Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05).					
32538719	8	30	theme	Quantitative	786:797	arg1	PCR					809:811	Quantitative real-time PCR	786:811	Quantitative real-time PCR	786:811	Quantitative real-time PCR was performed on cDNA samples using the SYBR™ Green PCR Master Mix.					
32538719	19	31	theme	fatty	1962:1966	arg1	acids					1968:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids	1900:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues	1900:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	7	32	theme	total	662:666	arg1	RNA					668:670	The total RNA	658:670	The total RNA from the mesenteric lymph node	658:701	The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit.					
32538719	11	33	theme	fecal	1047:1051	arg1	samples					1053:1059	fecal samples	1047:1059	fecal samples	1047:1059	16S rDNA sequencing analysis of fecal samples showed that supplementation with S. sieboldii MIQ.					
32538719	8	34	theme	SYBR™	853:857	arg1	Mix					876:878	the SYBR™ Green PCR Master Mix	849:878	the SYBR™ Green PCR Master Mix	849:878	Quantitative real-time PCR was performed on cDNA samples using the SYBR™ Green PCR Master Mix.					
32538719	6	35	theme	data	647:650	arg1	base					652:655	EzBioCloud data base	636:655	EzBioCloud data base	636:655	MATERIALS AND METHODS 16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed using EzBioCloud data base.					
32538719	22	36	theme	supplemented	2298:2309	arg1	mice					2311:2314	supplemented mice	2298:2314	supplemented mice	2298:2314	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	8	37	theme	PCR	865:867	arg1	Mix					876:878	the SYBR™ Green PCR Master Mix	849:878	the SYBR™ Green PCR Master Mix	849:878	Quantitative real-time PCR was performed on cDNA samples using the SYBR™ Green PCR Master Mix.					
32538719	7	38	from	node	698:701	arg1	RNA					668:670	The total RNA	658:670	The total RNA from the mesenteric lymph node	658:701	The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit.					
32538719	21	39	theme	harmful	2142:2148	arg1	microflora					2150:2159	decreased harmful microflora	2132:2159	decreased harmful microflora	2132:2159	increased beneficial gut microflora and decreased harmful microflora.					
32538719	16	40	theme	6:0	1618:1620	arg1	percentage					1604:1613	a higher percentage	1595:1613	a higher percentage of 6:0 and 18:2n-6	1595:1632	group showed a higher percentage of 6:0 and 18:2n-6 compared with that in the control group (P<0.05).					
32538719	21	41	theme	increased	2092:2100	arg1	microflora					2117:2126	increased beneficial gut microflora	2092:2126	increased beneficial gut microflora	2092:2126	increased beneficial gut microflora and decreased harmful microflora.					
32538719	16	42	theme	18:2n-6	1626:1632	arg1	percentage					1604:1613	a higher percentage	1595:1613	a higher percentage of 6:0 and 18:2n-6	1595:1632	group showed a higher percentage of 6:0 and 18:2n-6 compared with that in the control group (P<0.05).					
32538719	22	43	theme	mesenteric	2273:2282	arg1	node					2290:2293	the mesenteric lymph node	2269:2293	the mesenteric lymph node of supplemented mice	2269:2314	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	21	44	theme	gut	2113:2115	arg1	microflora					2117:2126	increased beneficial gut microflora	2092:2126	increased beneficial gut microflora	2092:2126	increased beneficial gut microflora and decreased harmful microflora.					
32538719	1	45	theme	Cytokine	85:92	arg1	Expression					94:103	Cytokine Expression	85:103	Cytokine Expression	85:103	Supplementation on Modulating Gut Microflora and Cytokine Expression in Mice.					
32538719	2	46	theme	MIQ	166:168	arg1	intake					138:143	The intake	134:143	AIMS AND OBJECTIVES The intake of Stachys sieboldii MIQ.	114:169	AIMS AND OBJECTIVES The intake of Stachys sieboldii MIQ.					
32538719	15	47	theme	acid	1540:1543	arg1	composition					1545:1555	The fecal fatty acid composition	1524:1555	The fecal fatty acid composition in the S. sieboldii MIQ.	1524:1580	The fecal fatty acid composition in the S. sieboldii MIQ.					
32538719	0	48	theme	sieboldii	21:29	arg1	Effect					4:9	The Effect	0:9	The Effect of Stachy sieboldii	0:29	The Effect of Stachy sieboldii MIQ.					
32538719	17	49	theme	S.	1783:1784	arg1	MIQ					1796:1798	S. sieboldii MIQ	1783:1798	S. sieboldii MIQ	1783:1798	The percentages of 6:0 and 20:3n-6 fatty acids were also significantly higher in the intestines of S. sieboldii MIQ.					
32538719	13	50	dep	control	1355:1361	arg1	P<0.05					1364:1369	P<0.05	1364:1369	P<0.05 for all	1364:1377	in feces compared with that in the control (P<0.05 for all).					
32538719	10	51	theme	coliform	970:977	arg1	counts					948:953	significantly reduced counts	926:953	significantly reduced counts of aerobic and coliform	926:977	showed significantly reduced counts of aerobic and coliform in the feces compared with control.					
32538719	4	52	theme	sieboldii	339:347	arg1	mice					358:361	S. sieboldii MIQ.-fed mice	336:361	S. sieboldii MIQ.-fed mice	336:361	We investigated the diversity and composition of microflora in feces of S. sieboldii MIQ.-fed mice.					
32538719	15	53	theme	fecal	1528:1532	arg1	composition					1545:1555	The fecal fatty acid composition	1524:1555	The fecal fatty acid composition in the S. sieboldii MIQ.	1524:1580	The fecal fatty acid composition in the S. sieboldii MIQ.					
32538719	12	54	theme	beneficial	1122:1131	arg1	microflora					1144:1153	beneficial intestinal microflora	1122:1153	beneficial intestinal microflora	1122:1153	increased beneficial intestinal microflora (Ruminococcaceae and Akkermansia muciniphila) and decreased the community of harmful microflora (Enterobacteriaceae, including Escherichia coli and Bacteroides sp.)					
32538719	7	55	theme	synthesis	771:779	arg1	kit					781:783	prime script 1st strand cDNA synthesis kit	742:783	prime script 1st strand cDNA synthesis kit	742:783	The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit.					
32538719	17	56	theme	MIQ	1796:1798	arg1	intestines					1769:1778	the intestines	1765:1778	the intestines of S. sieboldii MIQ	1765:1798	The percentages of 6:0 and 20:3n-6 fatty acids were also significantly higher in the intestines of S. sieboldii MIQ.					
32538719	10	57	theme	aerobic	958:964	arg1	counts					948:953	significantly reduced counts	926:953	significantly reduced counts of aerobic and coliform	926:977	showed significantly reduced counts of aerobic and coliform in the feces compared with control.					
32538719	4	58	theme	mice	358:361	arg1	feces					327:331	feces	327:331	feces of S. sieboldii MIQ.-fed mice	327:361	We investigated the diversity and composition of microflora in feces of S. sieboldii MIQ.-fed mice.					
32538719	19	59	theme	saturated	1906:1914	arg1	acids					1968:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids	1900:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues	1900:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	2	60	dep	AIMS	114:117	arg1	intake					138:143	The intake	134:143	AIMS AND OBJECTIVES The intake of Stachys sieboldii MIQ.	114:169	AIMS AND OBJECTIVES The intake of Stachys sieboldii MIQ.					
32538719	7	61	theme	strand	759:764	arg1	kit					781:783	prime script 1st strand cDNA synthesis kit	742:783	prime script 1st strand cDNA synthesis kit	742:783	The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit.					
32538719	11	62	with	supplementation	1073:1087	arg1	MIQ					1107:1109	S. sieboldii MIQ	1094:1109	S. sieboldii MIQ	1094:1109	16S rDNA sequencing analysis of fecal samples showed that supplementation with S. sieboldii MIQ.					
32538719	20	63	with	supplementation	2045:2059	arg1	MIQ					2087:2089	S. sieboldii MIQ	2074:2089	S. sieboldii MIQ	2074:2089	CONCLUSION The present results showed that supplementation of mice with S. sieboldii MIQ.					
32538719	17	64	theme	20:3n-6	1711:1717	arg1	acids					1725:1729	6:0 and 20:3n-6 fatty acids	1703:1729	6:0 and 20:3n-6 fatty acids	1703:1729	The percentages of 6:0 and 20:3n-6 fatty acids were also significantly higher in the intestines of S. sieboldii MIQ.					
32538719	9	65	theme	sieboldii	904:912	arg1	MIQ					914:916	S. sieboldii MIQ	901:916	S. sieboldii MIQ	901:916	RESULTS Mice fed on S. sieboldii MIQ.					
32538719	7	66	theme	script	748:753	arg1	kit					781:783	prime script 1st strand cDNA synthesis kit	742:783	prime script 1st strand cDNA synthesis kit	742:783	The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit.					
32538719	12	67	theme	harmful	1232:1238	arg1	microflora					1240:1249	harmful microflora	1232:1249	harmful microflora	1232:1249	increased beneficial intestinal microflora (Ruminococcaceae and Akkermansia muciniphila) and decreased the community of harmful microflora (Enterobacteriaceae, including Escherichia coli and Bacteroides sp.)					
32538719	4	68	dep	diversity	284:292	arg1	the					280:282	the	280:282	the	280:282	We investigated the diversity and composition of microflora in feces of S. sieboldii MIQ.-fed mice.					
32538719	1	69	from	Microflora	70:79	arg1	Mice					108:111	Mice	108:111	Mice	108:111	Supplementation on Modulating Gut Microflora and Cytokine Expression in Mice.					
32538719	22	70	theme	lower	2172:2176	arg1	expression					2183:2192	lower mRNA expression	2172:2192	lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice	2172:2314	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	2	71	theme	Stachys	148:154	arg1	MIQ					166:168	Stachys sieboldii MIQ	148:168	Stachys sieboldii MIQ	148:168	AIMS AND OBJECTIVES The intake of Stachys sieboldii MIQ.					
32538719	14	72	theme	lymph	1473:1477	arg1	node					1479:1482	mesenteric lymph node	1462:1482	mesenteric lymph node	1462:1482	Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05).					
32538719	22	73	from	expression	2183:2192	arg1	node					2290:2293	the mesenteric lymph node	2269:2293	the mesenteric lymph node of supplemented mice	2269:2314	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	17	74	theme	fatty	1719:1723	arg1	acids					1725:1729	6:0 and 20:3n-6 fatty acids	1703:1729	6:0 and 20:3n-6 fatty acids	1703:1729	The percentages of 6:0 and 20:3n-6 fatty acids were also significantly higher in the intestines of S. sieboldii MIQ.					
32538719	15	75	theme	S.	1564:1565	arg1	MIQ					1577:1579	the S. sieboldii MIQ	1560:1579	the S. sieboldii MIQ	1560:1579	The fecal fatty acid composition in the S. sieboldii MIQ.					
32538719	5	76	theme	acid	482:485	arg1	composition					487:497	fatty acid composition	476:497	fatty acid composition	476:497	In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.					
32538719	20	77	theme	S.	2074:2075	arg1	MIQ					2087:2089	S. sieboldii MIQ	2074:2089	S. sieboldii MIQ	2074:2089	CONCLUSION The present results showed that supplementation of mice with S. sieboldii MIQ.					
32538719	15	78	from	composition	1545:1555	arg1	MIQ					1577:1579	the S. sieboldii MIQ	1560:1579	the S. sieboldii MIQ	1560:1579	The fecal fatty acid composition in the S. sieboldii MIQ.					
32538719	11	79	theme	samples	1053:1059	arg1	analysis					1035:1042	16S rDNA sequencing analysis	1015:1042	16S rDNA sequencing analysis of fecal samples	1015:1059	16S rDNA sequencing analysis of fecal samples showed that supplementation with S. sieboldii MIQ.					
32538719	22	80	theme	cytokine	2251:2258	arg1	IL-10					2260:2264	anti-inflammatory cytokine IL-10	2233:2264	anti-inflammatory cytokine IL-10	2233:2264	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	3	81	theme	gut	243:245	arg1	function					254:261	optimal gut health function	235:261	optimal gut health function	235:261	has been associated with relieving inflammation and maintaining optimal gut health function.					
32538719	17	82	theme	6:0	1703:1705	arg1	acids					1725:1729	6:0 and 20:3n-6 fatty acids	1703:1729	6:0 and 20:3n-6 fatty acids	1703:1729	The percentages of 6:0 and 20:3n-6 fatty acids were also significantly higher in the intestines of S. sieboldii MIQ.					
32538719	14	83	theme	cytokines	1434:1442	arg1	expression					1420:1429	a significantly lower mRNA expression	1393:1429	a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node	1393:1482	Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05).					
32538719	12	84	dep	including	1272:1280	arg1	Enterobacteriaceae					1252:1269	Enterobacteriaceae	1252:1269	Enterobacteriaceae	1252:1269	increased beneficial intestinal microflora (Ruminococcaceae and Akkermansia muciniphila) and decreased the community of harmful microflora (Enterobacteriaceae, including Escherichia coli and Bacteroides sp.)					
32538719	11	85	theme	sieboldii	1097:1105	arg1	MIQ					1107:1109	S. sieboldii MIQ	1094:1109	S. sieboldii MIQ	1094:1109	16S rDNA sequencing analysis of fecal samples showed that supplementation with S. sieboldii MIQ.					
32538719	22	86	theme	microflora	2379:2388	arg1	abundances					2351:2360	the lower abundances	2341:2360	the lower abundances of harmful fecal microflora	2341:2388	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	17	87	theme	acids	1725:1729	arg1	higher					1755:1760	higher	1755:1760	higher	1755:1760	The percentages of 6:0 and 20:3n-6 fatty acids were also significantly higher in the intestines of S. sieboldii MIQ.					
32538719	17	87	theme	acids	1725:1729	arg1	percentages					1688:1698	The percentages	1684:1698	The percentages of 6:0 and 20:3n-6 fatty acids	1684:1729	The percentages of 6:0 and 20:3n-6 fatty acids were also significantly higher in the intestines of S. sieboldii MIQ.					
32538719	6	88	theme	taxonomic	587:595	arg1	analysis					607:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	MATERIALS AND METHODS 16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed using EzBioCloud data base.					
32538719	20	89	theme	mice	2064:2067	arg1	supplementation					2045:2059	supplementation	2045:2059	supplementation of mice with S. sieboldii MIQ	2045:2089	CONCLUSION The present results showed that supplementation of mice with S. sieboldii MIQ.					
32538719	22	90	theme	IL-6	2223:2226	arg1	expression					2183:2192	lower mRNA expression	2172:2192	lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice	2172:2314	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	15	91	dep	S.	1564:1565	arg1	sieboldii					1567:1575	sieboldii	1567:1575	sieboldii	1567:1575	The fecal fatty acid composition in the S. sieboldii MIQ.					
32538719	6	92	theme	sequencing-based	559:574	arg1	analysis					607:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	MATERIALS AND METHODS 16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed using EzBioCloud data base.					
32538719	11	93	theme	16S	1015:1017	arg1	sequencing					1024:1033	16S rDNA sequencing	1015:1033	16S rDNA sequencing analysis of fecal samples	1015:1059	16S rDNA sequencing analysis of fecal samples showed that supplementation with S. sieboldii MIQ.					
32538719	22	94	theme	pro-inflammatory	2197:2212	arg1	IL-6					2223:2226	pro-inflammatory cytokine IL-6	2197:2226	pro-inflammatory cytokine IL-6	2197:2226	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	7	95	theme	lymph	692:696	arg1	node					698:701	the mesenteric lymph node	677:701	the mesenteric lymph node	677:701	The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit.					
32538719	22	96	theme	fecal	2373:2377	arg1	microflora					2379:2388	harmful fecal microflora	2365:2388	harmful fecal microflora	2365:2388	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	19	97	theme	polyunsaturated	1946:1960	arg1	acids					1968:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids	1900:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues	1900:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	5	98	theme	tissues	510:516	arg1	inflammation					459:470	inflammation	459:470	inflammation	459:470	In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.					
32538719	5	98	theme	tissues	510:516	arg1	composition					487:497	fatty acid composition	476:497	fatty acid composition	476:497	In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.					
32538719	22	99	theme	mice	2311:2314	arg1	node					2290:2293	the mesenteric lymph node	2269:2293	the mesenteric lymph node of supplemented mice	2269:2314	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	19	100	from	groups	1862:1867	arg1	terms					1872:1876	terms	1872:1876	terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues	1872:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	11	101	theme	sequencing	1024:1033	arg1	analysis					1035:1042	16S rDNA sequencing analysis	1015:1042	16S rDNA sequencing analysis of fecal samples	1015:1059	16S rDNA sequencing analysis of fecal samples showed that supplementation with S. sieboldii MIQ.					
32538719	21	102	theme	decreased	2132:2140	arg1	microflora					2150:2159	decreased harmful microflora	2132:2159	decreased harmful microflora	2132:2159	increased beneficial gut microflora and decreased harmful microflora.					
32538719	6	103	theme	EzBioCloud	636:645	arg1	base					652:655	EzBioCloud data base	636:655	EzBioCloud data base	636:655	MATERIALS AND METHODS 16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed using EzBioCloud data base.					
32538719	8	104	theme	real-time	799:807	arg1	PCR					809:811	Quantitative real-time PCR	786:811	Quantitative real-time PCR	786:811	Quantitative real-time PCR was performed on cDNA samples using the SYBR™ Green PCR Master Mix.					
32538719	16	105	theme	higher	1597:1602	arg1	percentage					1604:1613	a higher percentage	1595:1613	a higher percentage of 6:0 and 18:2n-6	1595:1632	group showed a higher percentage of 6:0 and 18:2n-6 compared with that in the control group (P<0.05).					
32538719	8	106	theme	Green	859:863	arg1	Mix					876:878	the SYBR™ Green PCR Master Mix	849:878	the SYBR™ Green PCR Master Mix	849:878	Quantitative real-time PCR was performed on cDNA samples using the SYBR™ Green PCR Master Mix.					
32538719	19	107	theme	acids	1968:1972	arg1	percentages					1885:1895	the percentages	1881:1895	the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues	1881:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	22	108	theme	lymph	2284:2288	arg1	node					2290:2293	the mesenteric lymph node	2269:2293	the mesenteric lymph node of supplemented mice	2269:2314	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	1	109	theme	Gut	66:68	arg1	Microflora					70:79	Gut Microflora	66:79	Gut Microflora	66:79	Supplementation on Modulating Gut Microflora and Cytokine Expression in Mice.					
32538719	8	110	theme	Master	869:874	arg1	Mix					876:878	the SYBR™ Green PCR Master Mix	849:878	the SYBR™ Green PCR Master Mix	849:878	Quantitative real-time PCR was performed on cDNA samples using the SYBR™ Green PCR Master Mix.					
32538719	21	111	theme	beneficial	2102:2111	arg1	microflora					2117:2126	increased beneficial gut microflora	2092:2126	increased beneficial gut microflora	2092:2126	increased beneficial gut microflora and decreased harmful microflora.					
32538719	5	112	dep	cytokines	414:422	arg1	-10					443:445	-10	443:445	-10	443:445	In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.					
32538719	5	112	dep	cytokines	414:422	arg1	Interleukin-6					425:437	Interleukin-6	425:437	Interleukin-6	425:437	In addition, we evaluated the production of major cytokines (Interleukin-6 and -10) related to inflammation and fatty acid composition of several tissues.					
32538719	14	113	dep	cytokines	1434:1442	arg1	cytokines					1434:1442	cytokines	1434:1442	cytokines IL-6 and IL-10	1434:1457	Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05).					
32538719	14	113	dep	cytokines	1434:1442	arg1	IL-10					1453:1457	IL-10	1453:1457	IL-10	1453:1457	Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05).					
32538719	14	113	dep	cytokines	1434:1442	arg1	IL-6					1444:1447	IL-6	1444:1447	IL-6	1444:1447	Mice showed a significantly lower mRNA expression of cytokines IL-6 and IL-10 in mesenteric lymph node compared with that in control (P<0.05).					
32538719	6	114	theme	ribosomal	545:553	arg1	analysis					607:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	ribosomal DNA sequencing-based microbiome taxonomic profiling analysis	545:614	MATERIALS AND METHODS 16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed using EzBioCloud data base.					
32538719	0	115	theme	Stachy	14:19	arg1	sieboldii					21:29	Stachy sieboldii	14:29	Stachy sieboldii	14:29	The Effect of Stachy sieboldii MIQ.					
32538719	10	116	theme	reduced	940:946	arg1	counts					948:953	significantly reduced counts	926:953	significantly reduced counts of aerobic and coliform	926:977	showed significantly reduced counts of aerobic and coliform in the feces compared with control.					
32538719	12	117	theme	Bacteroides	1303:1313	arg1	sp					1315:1316	Bacteroides sp	1303:1316	Bacteroides sp	1303:1316	increased beneficial intestinal microflora (Ruminococcaceae and Akkermansia muciniphila) and decreased the community of harmful microflora (Enterobacteriaceae, including Escherichia coli and Bacteroides sp.)					
32538719	15	118	theme	fatty	1534:1538	arg1	composition					1545:1555	The fecal fatty acid composition	1524:1555	The fecal fatty acid composition in the S. sieboldii MIQ.	1524:1580	The fecal fatty acid composition in the S. sieboldii MIQ.					
32538719	22	119	theme	lower	2345:2349	arg1	abundances					2351:2360	the lower abundances	2341:2360	the lower abundances of harmful fecal microflora	2341:2388	Moreover, lower mRNA expression of pro-inflammatory cytokine IL-6, and anti-inflammatory cytokine IL-10 in the mesenteric lymph node of supplemented mice might be associated with the lower abundances of harmful fecal microflora.					
32538719	20	120	theme	present	2017:2023	arg1	results					2025:2031	The present results	2013:2031	The present results	2013:2031	CONCLUSION The present results showed that supplementation of mice with S. sieboldii MIQ.					
32538719	9	121	theme	RESULTS	881:887	arg1	Mice					889:892	RESULTS Mice	881:892	RESULTS Mice	881:892	RESULTS Mice fed on S. sieboldii MIQ.					
32538719	2	122	theme	sieboldii	156:164	arg1	MIQ					166:168	Stachys sieboldii MIQ	148:168	Stachys sieboldii MIQ	148:168	AIMS AND OBJECTIVES The intake of Stachys sieboldii MIQ.					
32538719	12	123	theme	intestinal	1133:1142	arg1	microflora					1144:1153	beneficial intestinal microflora	1122:1153	beneficial intestinal microflora	1122:1153	increased beneficial intestinal microflora (Ruminococcaceae and Akkermansia muciniphila) and decreased the community of harmful microflora (Enterobacteriaceae, including Escherichia coli and Bacteroides sp.)					
32538719	17	124	theme	sieboldii	1786:1794	arg1	MIQ					1796:1798	S. sieboldii MIQ	1783:1798	S. sieboldii MIQ	1783:1798	The percentages of 6:0 and 20:3n-6 fatty acids were also significantly higher in the intestines of S. sieboldii MIQ.					
32538719	4	125	theme	S.	336:337	arg1	mice					358:361	S. sieboldii MIQ.-fed mice	336:361	S. sieboldii MIQ.-fed mice	336:361	We investigated the diversity and composition of microflora in feces of S. sieboldii MIQ.-fed mice.					
32538719	19	126	theme	total	1900:1904	arg1	acids					1968:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids	1900:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues	1900:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	1	127	from	Expression	94:103	arg1	Mice					108:111	Mice	108:111	Mice	108:111	Supplementation on Modulating Gut Microflora and Cytokine Expression in Mice.					
32538719	4	128	theme	MIQ.-fed	349:356	arg1	mice					358:361	S. sieboldii MIQ.-fed mice	336:361	S. sieboldii MIQ.-fed mice	336:361	We investigated the diversity and composition of microflora in feces of S. sieboldii MIQ.-fed mice.					
32538719	19	129	theme	percentages	1885:1895	arg1	terms					1872:1876	terms	1872:1876	terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues	1872:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	9	130	theme	S.	901:902	arg1	MIQ					914:916	S. sieboldii MIQ	901:916	S. sieboldii MIQ	901:916	RESULTS Mice fed on S. sieboldii MIQ.					
32538719	19	131	theme	n-6	1934:1936	arg1	acids					1968:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids	1900:1972	total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues	1900:1999	No differences were revealed between the two groups in terms of the percentages of total saturated, monounsaturated, n-6 and n-3 polyunsaturated fatty acids found in feces and tissues.					
32538719	7	132	theme	cDNA	766:769	arg1	kit					781:783	prime script 1st strand cDNA synthesis kit	742:783	prime script 1st strand cDNA synthesis kit	742:783	The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit.					
32538719	4	133	theme	microflora	313:322	arg1	composition					298:308	composition	298:308	composition	298:308	We investigated the diversity and composition of microflora in feces of S. sieboldii MIQ.-fed mice.					
32538719	4	133	theme	microflora	313:322	arg1	diversity					284:292	diversity	284:292	diversity	284:292	We investigated the diversity and composition of microflora in feces of S. sieboldii MIQ.-fed mice.					
32538719	12	134	theme	microflora	1240:1249	arg1	community					1219:1227	the community	1215:1227	the community of harmful microflora	1215:1249	increased beneficial intestinal microflora (Ruminococcaceae and Akkermansia muciniphila) and decreased the community of harmful microflora (Enterobacteriaceae, including Escherichia coli and Bacteroides sp.)					
32538719	20	135	dep	showed	2033:2038	arg1	supplementation					2045:2059	supplementation	2045:2059	supplementation of mice with S. sieboldii MIQ	2045:2089	CONCLUSION The present results showed that supplementation of mice with S. sieboldii MIQ.					
32538719	7	136	theme	1st	755:757	arg1	kit					781:783	prime script 1st strand cDNA synthesis kit	742:783	prime script 1st strand cDNA synthesis kit	742:783	The total RNA from the mesenteric lymph node was isolated and then synthesized with prime script 1st strand cDNA synthesis kit.					
32561281	0	0	theme	frondosa	98:105	arg1	growth					51:56	mycelia growth	43:56	mycelia growth	43:56	UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa.					
32561281	0	0	theme	frondosa	98:105	arg1	synthesis					77:85	polysaccharide synthesis	62:85	polysaccharide synthesis	62:85	UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa.					
32561281	4	1	theme	metabolism	998:1007	arg1	alteration					978:987	alteration	978:987	alteration of sugar metabolism	978:1007	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	4	1	theme	metabolism	998:1007	arg1	silence					966:972	gfugp silence	960:972	gfugp silence	960:972	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	2	2	theme	silencing	385:393	arg1	vectors					395:401	Two dual promoter RNA silencing vectors	363:401	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual	363:442	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively.					
32561281	1	3	theme	UDP-glucose	264:274	arg1	gene					294:297	a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp	245:303	a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein	245:332	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	0	4	theme	Grifola	90:96	arg1	frondosa					98:105	Grifola frondosa	90:105	Grifola frondosa	90:105	UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa.					
32561281	4	5	theme	sugar	992:996	arg1	metabolism					998:1007	sugar metabolism	992:1007	sugar metabolism	992:1007	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	2	6	theme	RNA	381:383	arg1	vectors					395:401	Two dual promoter RNA silencing vectors	363:401	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual	363:442	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively.					
32561281	1	7	theme	pyrophosphorylase	276:292	arg1	gene					294:297	a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp	245:303	a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein	245:332	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	3	8	with	efficiency	654:663	arg1	growth					686:691	slower mycelial growth	670:691	slower mycelial growth	670:691	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	3	8	with	efficiency	654:663	arg1	production					712:721	polysaccharide production	697:721	polysaccharide production	697:721	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	3	9	theme	promoter	607:614	arg1	sequence					616:623	gfugp promoter sequence	601:623	gfugp promoter sequence	601:623	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	4	10	theme	gfuxe	1090:1094	arg1	transcription					1097:1109	UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription	1030:1109	transcription	1097:1109	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	3	11	theme	sequence	616:623	arg1	silence					590:596	silence	590:596	silence of gfugp promoter sequence	590:623	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	4	12	theme	mycelial	804:811	arg1	compositions/percentages					776:799	The monosaccharide compositions/percentages	757:799	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides	757:835	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	5	13	dep	provide	1131:1137	arg1	would					1125:1129	would	1125:1129	would	1125:1129	Our findings would provide a reference to know the biosynthesis pathway of mushroom polysaccharides and improve their production by metabolic regulation.					
32561281	1	14	theme	mycelial	179:186	arg1	growth					188:193	mycelial growth	179:193	mycelial growth	179:193	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	4	15	theme	transcription	1097:1109	arg1	up-regulation					1013:1025	up-regulation	1013:1025	up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription	1013:1109	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	3	16	theme	gfugp	601:605	arg1	sequence					616:623	gfugp promoter sequence	601:623	gfugp promoter sequence	601:623	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	2	17	theme	pAN7-iUGP-C-dual	427:442	arg1	vectors					395:401	Two dual promoter RNA silencing vectors	363:401	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual	363:442	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively.					
32561281	0	18	theme	pyrophosphorylase	12:28	arg1	gene					30:33	UDP-glucose pyrophosphorylase gene	0:33	UDP-glucose pyrophosphorylase gene	0:33	UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa.					
32561281	3	19	theme	higher	631:636	arg1	efficiency					654:663	a higher down-regulating efficiency	629:663	a higher down-regulating efficiency with slower mycelial growth and polysaccharide production	629:721	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	4	20	theme	monosaccharide	761:774	arg1	compositions/percentages					776:799	The monosaccharide compositions/percentages	757:799	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides	757:835	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	0	21	theme	UDP-glucose	0:10	arg1	pyrophosphorylase					12:28	UDP-glucose pyrophosphorylase	0:28	UDP-glucose pyrophosphorylase gene	0:33	UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa.					
32561281	1	22	from	roles	131:135	arg1	growth					188:193	mycelial growth	179:193	mycelial growth	179:193	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	1	22	from	roles	131:135	arg1	synthesis					214:222	polysaccharide synthesis	199:222	polysaccharide synthesis	199:222	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	3	23	theme	down-regulating	638:652	arg1	efficiency					654:663	a higher down-regulating efficiency	629:663	a higher down-regulating efficiency with slower mycelial growth and polysaccharide production	629:721	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	1	24	theme	polysaccharide	199:212	arg1	synthesis					214:222	polysaccharide synthesis	199:222	polysaccharide synthesis	199:222	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	3	25	contain	had	625:627	arg2	efficiency					654:663	a higher down-regulating efficiency	629:663	a higher down-regulating efficiency with slower mycelial growth and polysaccharide production	629:721	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	3	25	contain	had	625:627	arg1	silence					590:596	silence	590:596	silence of gfugp promoter sequence	590:623	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	4	26	theme	galactose	880:888	arg1	increase					868:875	the increase	864:875	the increase of galactose and arabinose contents	864:911	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	4	27	theme	supply	950:955	arg1	block					929:933	block	929:933	block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription	929:1109	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	2	28	theme	gfugp	478:482	arg1	expression					484:493	gfugp expression	478:493	gfugp expression	478:493	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively.					
32561281	1	29	theme	53.17-kDa	316:324	arg1	protein					326:332	a 53.17-kDa protein	314:332	a 53.17-kDa protein	314:332	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	1	30	theme	potential	121:129	arg1	roles					131:135	potential roles	121:135	potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa	121:242	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	2	31	theme	functional	534:543	arg1	sequences					545:553	conserved functional sequences	524:553	conserved functional sequences	524:553	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively.					
32561281	4	32	theme	exo-polysaccharides	817:835	arg1	compositions/percentages					776:799	The monosaccharide compositions/percentages	757:799	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides	757:835	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	3	33	theme	conserved	737:745	arg1	sequence					747:754	conserved sequence	737:754	conserved sequence	737:754	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	3	34	theme	slower	670:675	arg1	growth					686:691	slower mycelial growth	670:691	slower mycelial growth	670:691	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	2	35	theme	pAN7-iUGP-P-dual	406:421	arg1	vectors					395:401	Two dual promoter RNA silencing vectors	363:401	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual	363:442	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively.					
32561281	1	36	theme	Grifola	227:233	arg1	frondosa					235:242	Grifola frondosa	227:242	Grifola frondosa	227:242	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	0	37	theme	mycelia	43:49	arg1	growth					51:56	mycelia growth	43:56	mycelia growth	43:56	UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa.					
32561281	2	38	theme	conserved	524:532	arg1	sequences					545:553	conserved functional sequences	524:553	conserved functional sequences	524:553	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively.					
32561281	3	39	theme	mycelial	677:684	arg1	growth					686:691	slower mycelial growth	670:691	slower mycelial growth	670:691	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	1	40	theme	frondosa	235:242	arg1	growth					188:193	mycelial growth	179:193	mycelial growth	179:193	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	1	40	theme	frondosa	235:242	arg1	synthesis					214:222	polysaccharide synthesis	199:222	polysaccharide synthesis	199:222	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	0	41	theme	polysaccharide	62:75	arg1	synthesis					77:85	polysaccharide synthesis	62:85	polysaccharide synthesis	62:85	UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa.					
32561281	4	42	theme	UDP-glucose-4-epimerase	1030:1052	arg1	gfuge					1055:1059	UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription	1030:1109	gfuge	1055:1059	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	5	43	theme	metabolic	1244:1252	arg1	regulation					1254:1263	metabolic regulation	1244:1263	metabolic regulation	1244:1263	Our findings would provide a reference to know the biosynthesis pathway of mushroom polysaccharides and improve their production by metabolic regulation.					
32561281	4	44	theme	gfugp	960:964	arg1	silence					966:972	gfugp silence	960:972	gfugp silence	960:972	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	2	45	theme	promoter	372:379	arg1	vectors					395:401	Two dual promoter RNA silencing vectors	363:401	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual	363:442	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively.					
32561281	4	46	theme	UDP-xylose-4-epimerase	1066:1087	arg1	transcription					1097:1109	UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription	1030:1109	transcription	1097:1109	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	5	47	theme	mushroom	1187:1194	arg1	polysaccharides					1196:1210	mushroom polysaccharides	1187:1210	mushroom polysaccharides	1187:1210	Our findings would provide a reference to know the biosynthesis pathway of mushroom polysaccharides and improve their production by metabolic regulation.					
32561281	5	48	theme	polysaccharides	1196:1210	arg1	pathway					1176:1182	the biosynthesis pathway	1159:1182	the biosynthesis pathway of mushroom polysaccharides	1159:1210	Our findings would provide a reference to know the biosynthesis pathway of mushroom polysaccharides and improve their production by metabolic regulation.					
32561281	2	49	theme	dual	367:370	arg1	vectors					395:401	Two dual promoter RNA silencing vectors	363:401	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual	363:442	Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively.					
32561281	4	50	theme	gfuge	1055:1059	arg1	up-regulation					1013:1025	up-regulation	1013:1025	up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription	1013:1109	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	5	51	theme	biosynthesis	1163:1174	arg1	pathway					1176:1182	the biosynthesis pathway	1159:1182	the biosynthesis pathway of mushroom polysaccharides	1159:1210	Our findings would provide a reference to know the biosynthesis pathway of mushroom polysaccharides and improve their production by metabolic regulation.					
32561281	3	52	theme	polysaccharide	697:710	arg1	production					712:721	polysaccharide production	697:721	polysaccharide production	697:721	Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence.					
32561281	4	53	theme	UDP-glucose	938:948	arg1	supply					950:955	UDP-glucose supply	938:955	UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription	938:1109	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	1	54	theme	UDP-glucose	140:150	arg1	UGP					171:173	UGP	171:173	UGP	171:173	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	1	54	theme	UDP-glucose	140:150	arg1	pyrophosphorylase					152:168	UDP-glucose pyrophosphorylase	140:168	UDP-glucose pyrophosphorylase (UGP)	140:174	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	4	55	theme	contents	904:911	arg1	increase					868:875	the increase	864:875	the increase of galactose and arabinose contents	864:911	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	4	56	theme	arabinose	894:902	arg1	contents					904:911	arabinose contents	894:911	arabinose contents	894:911	The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription.					
32561281	1	57	theme	pyrophosphorylase	152:168	arg1	roles					131:135	potential roles	121:135	potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa	121:242	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	1	58	theme	putative	247:254	arg1	gene					294:297	a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp	245:303	a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein	245:332	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32561281	1	59	theme	2036-bp	256:262	arg1	gene					294:297	a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp	245:303	a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein	245:332	To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated.					
32690555	1	0	theme	lipopolysaccharide	258:275	arg1	component					245:253	the O antigen component	231:253	the O antigen component of lipopolysaccharide (LPS)	231:281	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	10	1	from	biosynthesis	1712:1723	arg1	aeruginosa					1731:1740	P. aeruginosa	1728:1740	P. aeruginosa	1728:1740	The findings in this report are critical for a comprehensive understanding of O antigen biosynthesis in P. aeruginosa and provide a framework for future studies.IMPORTANCEP.					
32690555	15	2	theme	O	2497:2497	arg1	biosynthesis					2507:2518	O antigen biosynthesis	2497:2518	O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity	2497:2594	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	5	3	theme	antigens	787:794	arg1	18					773:774	18	773:774	18	773:774	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	5	3	theme	antigens	787:794	arg1	antigens					787:794	these O antigens	779:794	these O antigens	779:794	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	4	4	theme	known	692:696	arg1	structures					723:732	20 known O-specific antigen (OSA) structures	689:732	20 known O-specific antigen (OSA) structures	689:732	In the opportunistic pathogen Pseudomonas aeruginosa, there are currently 20 known O-specific antigen (OSA) structures.					
32690555	14	5	theme	horizontal	2389:2398	arg1	transfer					2405:2412	extensive horizontal gene transfer	2379:2412	extensive horizontal gene transfer	2379:2412	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	1	6	theme	bacterial	191:199	arg1	component					245:253	the O antigen component	231:253	the O antigen component of lipopolysaccharide (LPS)	231:281	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	1	6	theme	bacterial	191:199	arg1	glycans					214:220	Many bacterial cell surface glycans	186:220	Many bacterial cell surface glycans	186:220	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	4	7	theme	antigen	709:715	arg1	structures					723:732	20 known O-specific antigen (OSA) structures	689:732	20 known O-specific antigen (OSA) structures	689:732	In the opportunistic pathogen Pseudomonas aeruginosa, there are currently 20 known O-specific antigen (OSA) structures.					
32690555	3	8	theme	O	602:602	arg1	serotypes					604:612	O serotypes	602:612	O serotypes	602:612	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	1	9	theme	surface	206:212	arg1	component					245:253	the O antigen component	231:253	the O antigen component of lipopolysaccharide (LPS)	231:281	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	1	9	theme	surface	206:212	arg1	glycans					214:220	Many bacterial cell surface glycans	186:220	Many bacterial cell surface glycans	186:220	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	12	10	theme	serotype-specific	2024:2040	arg1	antigen					2044:2050	a serotype-specific O antigen	2022:2050	a serotype-specific O antigen	2022:2050	This bacterium produces a number of virulence factors, including a serotype-specific O antigen.					
32690555	6	11	theme	biosynthesis	960:971	arg1	clusters					973:980	the two unidentified O antigen biosynthesis clusters	929:980	the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures	929:1048	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	11	12	theme	opportunistic	1826:1838	arg1	pathogen					1840:1847	a problematic opportunistic pathogen	1812:1847	a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis	1812:1954	aeruginosa is a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis.					
32690555	11	12	theme	opportunistic	1826:1838	arg1	aeruginosa					1798:1807	aeruginosa	1798:1807	aeruginosa	1798:1807	aeruginosa is a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis.					
32690555	0	13	theme	Synthesis	131:139	arg1	Pathway					141:147	an ABC Transporter-Dependent Synthesis Pathway	102:147	an ABC Transporter-Dependent Synthesis Pathway	102:147	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	5	14	located	located	876:882	arg2	which					825:829	which	825:829	which	825:829	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	5	14	located	located	876:882	arg1	locus					896:900	a common locus	887:900	a common locus	887:900	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	5	14	located	located	876:882	arg2	all					818:820	all	818:820	all	818:820	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	3	15	theme	bacteria	588:595	arg1	basis					567:571	the basis	563:571	the basis of classifying bacteria into O serotypes	563:612	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	3	15	theme	bacteria	588:595	arg1	structure					519:527	specific structure	510:527	specific structure	510:527	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	3	15	theme	bacteria	588:595	arg1	composition					533:543	composition	533:543	composition	533:543	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	5	16	theme	Wzx/Wzy-dependent	842:858	arg1	pathway					860:866	the Wzx/Wzy-dependent pathway	838:866	the Wzx/Wzy-dependent pathway	838:866	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	6	17	theme	O	950:950	arg1	clusters					973:980	the two unidentified O antigen biosynthesis clusters	929:980	the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures	929:1048	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	7	18	theme	OSA	1163:1165	arg1	locus					1180:1184	the conserved OSA biosynthesis locus	1149:1184	the conserved OSA biosynthesis locus	1149:1184	Intriguingly, these clusters were found outside the conserved OSA biosynthesis locus and were likely acquired through multiple horizontal gene transfer events.					
32690555	12	19	theme	factors	2003:2009	arg1	number					1983:1988	a number	1981:1988	a number of virulence factors, including a serotype-specific O antigen	1981:2050	This bacterium produces a number of virulence factors, including a serotype-specific O antigen.					
32690555	12	19	theme	factors	2003:2009	arg1	antigen					2044:2050	a serotype-specific O antigen	2022:2050	a serotype-specific O antigen	2022:2050	This bacterium produces a number of virulence factors, including a serotype-specific O antigen.					
32690555	14	20	dep	P.	2425:2426	arg1	aeruginosa					2428:2437	aeruginosa	2428:2437	aeruginosa	2428:2437	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	15	21	from	bacteria	2537:2544	arg1	understanding					2480:2492	our understanding	2476:2492	our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity	2476:2594	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	15	22	theme	antigen	2499:2505	arg1	biosynthesis					2507:2518	O antigen biosynthesis	2497:2518	O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity	2497:2594	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	0	23	theme	Diversity	175:183	arg1	Mechanisms					153:162	Mechanisms	153:162	Mechanisms of Genetic Diversity	153:183	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	0	23	theme	Diversity	175:183	arg1	Pathway					141:147	an ABC Transporter-Dependent Synthesis Pathway	102:147	an ABC Transporter-Dependent Synthesis Pathway	102:147	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	4	24	dep	pathogen	636:643	arg1	aeruginosa					657:666	the opportunistic pathogen Pseudomonas aeruginosa	618:666	the opportunistic pathogen Pseudomonas aeruginosa	618:666	In the opportunistic pathogen Pseudomonas aeruginosa, there are currently 20 known O-specific antigen (OSA) structures.					
32690555	9	25	theme	polysaccharide	1512:1525	arg1	lengths					1533:1539	the O15 and O17 polysaccharide chain lengths	1496:1539	the O15 and O17 polysaccharide chain lengths	1496:1539	In addition, we collected evidence to show that the O15 and O17 polysaccharide chain lengths are regulated by molecular rulers with distinct and variable domain architectures.					
32690555	6	26	theme	O15	1019:1021	arg1	synthesis					1002:1010	the synthesis	998:1010	the synthesis of the O15 and the O17 OSA structures	998:1048	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	14	27	theme	specific	2332:2339	arg1	genes					2354:2358	specific biosynthesis genes	2332:2358	specific biosynthesis genes	2332:2358	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	7	28	theme	transfer	1244:1251	arg1	events					1253:1258	multiple horizontal gene transfer events	1219:1258	multiple horizontal gene transfer events	1219:1258	Intriguingly, these clusters were found outside the conserved OSA biosynthesis locus and were likely acquired through multiple horizontal gene transfer events.					
32690555	2	29	from	environment	442:452	arg1	bacteria					422:429	bacteria	422:429	bacteria from their environment	422:452	O antigens are highly diverse polysaccharides that protect bacteria from their environment and engage in important host-pathogen interactions.					
32690555	0	30	theme	Biosynthesis	76:87	arg1	Loci					89:92	the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci	18:92	the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci	18:92	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	1	31	theme	transporter-dependent	331:351	arg1	pathways					353:360	ABC transporter-dependent pathways	327:360	ABC transporter-dependent pathways	327:360	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	13	32	theme	O	2138:2138	arg1	antigens					2140:2147	the O15 and O17 O antigens	2122:2147	the O15 and O17 O antigens	2122:2147	Here, we identified and characterized the gene clusters that produce the O15 and O17 O antigens and show that they utilize a pathway for synthesis that is distinct from that of the 18 other known serotypes.					
32690555	15	33	theme	O	2576:2576	arg1	diversity					2586:2594	O antigen diversity	2576:2594	O antigen diversity	2576:2594	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	7	34	theme	multiple	1219:1226	arg1	events					1253:1258	multiple horizontal gene transfer events	1219:1258	multiple horizontal gene transfer events	1219:1258	Intriguingly, these clusters were found outside the conserved OSA biosynthesis locus and were likely acquired through multiple horizontal gene transfer events.					
32690555	5	35	theme	responsible	757:767	arg1	genes					751:755	genes	751:755	genes responsible for 18 of these O antigens	751:794	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	8	36	from	studies	1308:1314	arg1	data					1270:1273	data	1270:1273	data from knockout and overexpression studies	1270:1314	Based on data from knockout and overexpression studies, we determined that the synthesis of these O antigens follows an ABC transporter-dependent rather than a Wzx/Wzy-dependent pathway.					
32690555	9	37	theme	molecular	1558:1566	arg1	rulers					1568:1573	molecular rulers	1558:1573	molecular rulers with distinct and variable domain architectures	1558:1621	In addition, we collected evidence to show that the O15 and O17 polysaccharide chain lengths are regulated by molecular rulers with distinct and variable domain architectures.					
32690555	6	38	theme	responsible	982:992	arg1	clusters					973:980	the two unidentified O antigen biosynthesis clusters	929:980	the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures	929:1048	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	8	39	theme	antigens	1361:1368	arg1	synthesis					1340:1348	the synthesis	1336:1348	the synthesis of these O antigens	1336:1368	Based on data from knockout and overexpression studies, we determined that the synthesis of these O antigens follows an ABC transporter-dependent rather than a Wzx/Wzy-dependent pathway.					
32690555	10	40	theme	comprehensive	1671:1683	arg1	understanding					1685:1697	a comprehensive understanding	1669:1697	a comprehensive understanding of O antigen biosynthesis in P. aeruginosa	1669:1740	The findings in this report are critical for a comprehensive understanding of O antigen biosynthesis in P. aeruginosa and provide a framework for future studies.IMPORTANCEP.					
32690555	0	41	theme	ABC	105:107	arg1	Pathway					141:147	an ABC Transporter-Dependent Synthesis Pathway	102:147	an ABC Transporter-Dependent Synthesis Pathway	102:147	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	13	42	theme	gene	2095:2098	arg1	clusters					2100:2107	the gene clusters	2091:2107	the gene clusters that produce the O15 and O17 O antigens and show that they utilize a pathway for synthesis that is distinct from that of the 18 other known serotypes	2091:2257	Here, we identified and characterized the gene clusters that produce the O15 and O17 O antigens and show that they utilize a pathway for synthesis that is distinct from that of the 18 other known serotypes.					
32690555	6	43	theme	published	1063:1071	arg1	data					1095:1098	published whole-genome sequence data	1063:1098	published whole-genome sequence data	1063:1098	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	0	44	theme	Antigen	68:74	arg1	Biosynthesis					76:87	O15 O-Specific Antigen Biosynthesis	53:87	O15 O-Specific Antigen Biosynthesis	53:87	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	2	45	theme	diverse	385:391	arg1	polysaccharides					393:407	highly diverse polysaccharides	378:407	highly diverse polysaccharides that protect bacteria from their environment and engage in important host-pathogen interactions	378:503	O antigens are highly diverse polysaccharides that protect bacteria from their environment and engage in important host-pathogen interactions.					
32690555	2	45	theme	diverse	385:391	arg1	antigens					365:372	O antigens	363:372	O antigens	363:372	O antigens are highly diverse polysaccharides that protect bacteria from their environment and engage in important host-pathogen interactions.					
32690555	8	46	theme	transporter-dependent	1385:1405	arg1	ABC					1381:1383	an ABC transporter-dependent rather than a Wzx/Wzy-dependent pathway	1378:1445	an ABC transporter-dependent rather than a Wzx/Wzy-dependent pathway	1378:1445	Based on data from knockout and overexpression studies, we determined that the synthesis of these O antigens follows an ABC transporter-dependent rather than a Wzx/Wzy-dependent pathway.					
32690555	10	47	theme	antigen	1704:1710	arg1	biosynthesis					1712:1723	O antigen biosynthesis	1702:1723	O antigen biosynthesis in P. aeruginosa	1702:1740	The findings in this report are critical for a comprehensive understanding of O antigen biosynthesis in P. aeruginosa and provide a framework for future studies.IMPORTANCEP.					
32690555	7	48	theme	biosynthesis	1167:1178	arg1	locus					1180:1184	the conserved OSA biosynthesis locus	1149:1184	the conserved OSA biosynthesis locus	1149:1184	Intriguingly, these clusters were found outside the conserved OSA biosynthesis locus and were likely acquired through multiple horizontal gene transfer events.					
32690555	13	49	theme	known	2243:2247	arg1	serotypes					2249:2257	the 18 other known serotypes	2230:2257	the 18 other known serotypes	2230:2257	Here, we identified and characterized the gene clusters that produce the O15 and O17 O antigens and show that they utilize a pathway for synthesis that is distinct from that of the 18 other known serotypes.					
32690555	9	50	theme	domain	1602:1607	arg1	architectures					1609:1621	distinct and variable domain architectures	1580:1621	distinct and variable domain architectures	1580:1621	In addition, we collected evidence to show that the O15 and O17 polysaccharide chain lengths are regulated by molecular rulers with distinct and variable domain architectures.					
32690555	6	51	theme	OSA	1035:1037	arg1	structures					1039:1048	the O17 OSA structures	1027:1048	the O17 OSA structures	1027:1048	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	14	52	contain	have	2305:2308	arg2	mutations					2319:2327	acquired mutations	2310:2327	acquired mutations in specific biosynthesis genes	2310:2358	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	14	52	contain	have	2305:2308	arg1	clusters					2296:2303	these clusters	2290:2303	these clusters	2290:2303	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	7	53	theme	conserved	1153:1161	arg1	locus					1180:1184	the conserved OSA biosynthesis locus	1149:1184	the conserved OSA biosynthesis locus	1149:1184	Intriguingly, these clusters were found outside the conserved OSA biosynthesis locus and were likely acquired through multiple horizontal gene transfer events.					
32690555	2	54	theme	host-pathogen	478:490	arg1	interactions					492:503	important host-pathogen interactions	468:503	important host-pathogen interactions	468:503	O antigens are highly diverse polysaccharides that protect bacteria from their environment and engage in important host-pathogen interactions.					
32690555	0	55	theme	O-Specific	57:66	arg1	Biosynthesis					76:87	O15 O-Specific Antigen Biosynthesis	53:87	O15 O-Specific Antigen Biosynthesis	53:87	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	8	56	theme	Wzx/Wzy-dependent	1421:1437	arg1	pathway					1439:1445	a Wzx/Wzy-dependent pathway	1419:1445	an ABC transporter-dependent rather than a Wzx/Wzy-dependent pathway	1378:1445	Based on data from knockout and overexpression studies, we determined that the synthesis of these O antigens follows an ABC transporter-dependent rather than a Wzx/Wzy-dependent pathway.					
32690555	1	57	theme	so-called	305:313	arg1	Wzx/Wzy-					315:322	the so-called Wzx/Wzy-	301:322	the so-called Wzx/Wzy-	301:322	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	11	58	theme	compromised	1884:1894	arg1	defenses					1901:1908	compromised host defenses	1884:1908	compromised host defenses	1884:1908	aeruginosa is a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis.					
32690555	11	58	theme	compromised	1884:1894	arg1	those					1919:1923	those	1919:1923	those	1919:1923	aeruginosa is a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis.					
32690555	4	59	theme	opportunistic	622:634	arg1	pathogen					636:643	the opportunistic pathogen Pseudomonas aeruginosa	618:666	the opportunistic pathogen Pseudomonas aeruginosa	618:666	In the opportunistic pathogen Pseudomonas aeruginosa, there are currently 20 known O-specific antigen (OSA) structures.					
32690555	6	60	from	study	911:915	arg1	located					921:927	located	921:927	located	921:927	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	3	61	theme	O	548:548	arg1	antigens					550:557	O antigens	548:557	O antigens	548:557	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	1	62	theme	antigen	237:243	arg1	component					245:253	the O antigen component	231:253	the O antigen component of lipopolysaccharide (LPS)	231:281	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	4	63	theme	Pseudomonas	645:655	arg1	pathogen					636:643	the opportunistic pathogen Pseudomonas aeruginosa	618:666	the opportunistic pathogen Pseudomonas aeruginosa	618:666	In the opportunistic pathogen Pseudomonas aeruginosa, there are currently 20 known O-specific antigen (OSA) structures.					
32690555	5	64	theme	O	785:785	arg1	antigens					787:794	these O antigens	779:794	these O antigens	779:794	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	14	65	theme	P.	2425:2426	arg1	population					2439:2448	the P. aeruginosa population	2421:2448	the P. aeruginosa population	2421:2448	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	2	66	theme	O	363:363	arg1	polysaccharides					393:407	highly diverse polysaccharides	378:407	highly diverse polysaccharides that protect bacteria from their environment and engage in important host-pathogen interactions	378:503	O antigens are highly diverse polysaccharides that protect bacteria from their environment and engage in important host-pathogen interactions.					
32690555	2	66	theme	O	363:363	arg1	antigens					365:372	O antigens	363:372	O antigens	363:372	O antigens are highly diverse polysaccharides that protect bacteria from their environment and engage in important host-pathogen interactions.					
32690555	10	67	from	findings	1628:1635	arg1	report					1645:1650	this report	1640:1650	this report	1640:1650	The findings in this report are critical for a comprehensive understanding of O antigen biosynthesis in P. aeruginosa and provide a framework for future studies.IMPORTANCEP.					
32690555	6	68	theme	sequence	1086:1093	arg1	data					1095:1098	published whole-genome sequence data	1063:1098	published whole-genome sequence data	1063:1098	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	12	69	theme	O	2042:2042	arg1	antigen					2044:2050	a serotype-specific O antigen	2022:2050	a serotype-specific O antigen	2022:2050	This bacterium produces a number of virulence factors, including a serotype-specific O antigen.					
32690555	11	70	theme	host	1896:1899	arg1	defenses					1901:1908	compromised host defenses	1884:1908	compromised host defenses	1884:1908	aeruginosa is a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis.					
32690555	11	70	theme	host	1896:1899	arg1	those					1919:1923	those	1919:1923	those	1919:1923	aeruginosa is a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis.					
32690555	10	71	from	aeruginosa	1731:1740	arg1	understanding					1685:1697	a comprehensive understanding	1669:1697	a comprehensive understanding of O antigen biosynthesis in P. aeruginosa	1669:1740	The findings in this report are critical for a comprehensive understanding of O antigen biosynthesis in P. aeruginosa and provide a framework for future studies.IMPORTANCEP.					
32690555	14	72	theme	gene	2400:2403	arg1	transfer					2405:2412	extensive horizontal gene transfer	2379:2412	extensive horizontal gene transfer	2379:2412	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	10	73	theme	future	1770:1775	arg1	studies.IMPORTANCEP					1777:1795	future studies.IMPORTANCEP	1770:1795	future studies.IMPORTANCEP	1770:1795	The findings in this report are critical for a comprehensive understanding of O antigen biosynthesis in P. aeruginosa and provide a framework for future studies.IMPORTANCEP.					
32690555	1	74	theme	Many	186:189	arg1	component					245:253	the O antigen component	231:253	the O antigen component of lipopolysaccharide (LPS)	231:281	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	1	74	theme	Many	186:189	arg1	glycans					214:220	Many bacterial cell surface glycans	186:220	Many bacterial cell surface glycans	186:220	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	15	75	from	understanding	2480:2492	arg1	bacteria					2537:2544	Gram-negative bacteria	2523:2544	Gram-negative bacteria	2523:2544	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	15	75	from	understanding	2480:2492	arg1	mechanisms					2554:2563	the mechanisms	2550:2563	the mechanisms that drive O antigen diversity	2550:2594	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	7	76	theme	horizontal	1228:1237	arg1	events					1253:1258	multiple horizontal gene transfer events	1219:1258	multiple horizontal gene transfer events	1219:1258	Intriguingly, these clusters were found outside the conserved OSA biosynthesis locus and were likely acquired through multiple horizontal gene transfer events.					
32690555	14	77	theme	extensive	2379:2387	arg1	transfer					2405:2412	extensive horizontal gene transfer	2379:2412	extensive horizontal gene transfer	2379:2412	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	1	78	theme	cell	201:204	arg1	component					245:253	the O antigen component	231:253	the O antigen component of lipopolysaccharide (LPS)	231:281	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	1	78	theme	cell	201:204	arg1	glycans					214:220	Many bacterial cell surface glycans	186:220	Many bacterial cell surface glycans	186:220	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	4	79	theme	O-specific	698:707	arg1	OSA					718:720	OSA	718:720	OSA	718:720	In the opportunistic pathogen Pseudomonas aeruginosa, there are currently 20 known O-specific antigen (OSA) structures.					
32690555	4	79	theme	O-specific	698:707	arg1	antigen					709:715	O-specific antigen	698:715	20 known O-specific antigen (OSA) structures	689:732	In the opportunistic pathogen Pseudomonas aeruginosa, there are currently 20 known O-specific antigen (OSA) structures.					
32690555	3	80	theme	classifying	576:586	arg1	bacteria					588:595	classifying bacteria	576:595	classifying bacteria	576:595	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	6	81	theme	antigen	952:958	arg1	clusters					973:980	the two unidentified O antigen biosynthesis clusters	929:980	the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures	929:1048	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	15	82	from	biosynthesis	2507:2518	arg1	bacteria					2537:2544	Gram-negative bacteria	2523:2544	Gram-negative bacteria	2523:2544	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	15	82	from	biosynthesis	2507:2518	arg1	mechanisms					2554:2563	the mechanisms	2550:2563	the mechanisms that drive O antigen diversity	2550:2594	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	9	83	with	rulers	1568:1573	arg1	architectures					1609:1621	distinct and variable domain architectures	1580:1621	distinct and variable domain architectures	1580:1621	In addition, we collected evidence to show that the O15 and O17 polysaccharide chain lengths are regulated by molecular rulers with distinct and variable domain architectures.					
32690555	6	84	theme	unidentified	937:948	arg1	clusters					973:980	the two unidentified O antigen biosynthesis clusters	929:980	the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures	929:1048	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	8	85	theme	knockout	1280:1287	arg1	studies					1308:1314	knockout and overexpression studies	1280:1314	studies	1308:1314	Based on data from knockout and overexpression studies, we determined that the synthesis of these O antigens follows an ABC transporter-dependent rather than a Wzx/Wzy-dependent pathway.					
32690555	0	86	theme	Genetic	167:173	arg1	Diversity					175:183	Genetic Diversity	167:183	Genetic Diversity	167:183	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	15	87	theme	Gram-negative	2523:2535	arg1	bacteria					2537:2544	Gram-negative bacteria	2523:2544	Gram-negative bacteria	2523:2544	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	9	88	theme	O15	1500:1502	arg1	lengths					1533:1539	the O15 and O17 polysaccharide chain lengths	1496:1539	the O15 and O17 polysaccharide chain lengths	1496:1539	In addition, we collected evidence to show that the O15 and O17 polysaccharide chain lengths are regulated by molecular rulers with distinct and variable domain architectures.					
32690555	12	89	theme	virulence	1993:2001	arg1	factors					2003:2009	virulence factors	1993:2009	virulence factors	1993:2009	This bacterium produces a number of virulence factors, including a serotype-specific O antigen.					
32690555	12	89	theme	virulence	1993:2001	arg1	antigen					2044:2050	a serotype-specific O antigen	2022:2050	a serotype-specific O antigen	2022:2050	This bacterium produces a number of virulence factors, including a serotype-specific O antigen.					
32690555	8	90	theme	overexpression	1293:1306	arg1	studies					1308:1314	knockout and overexpression studies	1280:1314	studies	1308:1314	Based on data from knockout and overexpression studies, we determined that the synthesis of these O antigens follows an ABC transporter-dependent rather than a Wzx/Wzy-dependent pathway.					
32690555	13	91	theme	O17	2134:2136	arg1	antigens					2140:2147	the O15 and O17 O antigens	2122:2147	the O15 and O17 O antigens	2122:2147	Here, we identified and characterized the gene clusters that produce the O15 and O17 O antigens and show that they utilize a pathway for synthesis that is distinct from that of the 18 other known serotypes.					
32690555	15	92	theme	biosynthesis	2507:2518	arg1	understanding					2480:2492	our understanding	2476:2492	our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity	2476:2594	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	9	93	theme	O17	1508:1510	arg1	lengths					1533:1539	the O15 and O17 polysaccharide chain lengths	1496:1539	the O15 and O17 polysaccharide chain lengths	1496:1539	In addition, we collected evidence to show that the O15 and O17 polysaccharide chain lengths are regulated by molecular rulers with distinct and variable domain architectures.					
32690555	15	94	from	mechanisms	2554:2563	arg1	understanding					2480:2492	our understanding	2476:2492	our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity	2476:2594	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	14	95	theme	biosynthesis	2341:2352	arg1	genes					2354:2358	specific biosynthesis genes	2332:2358	specific biosynthesis genes	2332:2358	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	7	96	theme	gene	1239:1242	arg1	events					1253:1258	multiple horizontal gene transfer events	1219:1258	multiple horizontal gene transfer events	1219:1258	Intriguingly, these clusters were found outside the conserved OSA biosynthesis locus and were likely acquired through multiple horizontal gene transfer events.					
32690555	9	97	theme	chain	1527:1531	arg1	lengths					1533:1539	the O15 and O17 polysaccharide chain lengths	1496:1539	the O15 and O17 polysaccharide chain lengths	1496:1539	In addition, we collected evidence to show that the O15 and O17 polysaccharide chain lengths are regulated by molecular rulers with distinct and variable domain architectures.					
32690555	5	98	theme	common	889:894	arg1	locus					896:900	a common locus	887:900	a common locus	887:900	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	14	99	from	mutations	2319:2327	arg1	genes					2354:2358	specific biosynthesis genes	2332:2358	specific biosynthesis genes	2332:2358	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	0	100	theme	Loci	89:92	arg1	Identification					0:13	Identification	0:13	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci	0:92	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	1	101	theme	ABC	327:329	arg1	pathways					353:360	ABC transporter-dependent pathways	327:360	ABC transporter-dependent pathways	327:360	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	14	102	theme	acquired	2310:2317	arg1	mutations					2319:2327	acquired mutations	2310:2327	acquired mutations in specific biosynthesis genes	2310:2358	We also provide evidence that these clusters have acquired mutations in specific biosynthesis genes and have undergone extensive horizontal gene transfer within the P. aeruginosa population.					
32690555	11	103	theme	problematic	1814:1824	arg1	pathogen					1840:1847	a problematic opportunistic pathogen	1812:1847	a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis	1812:1954	aeruginosa is a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis.					
32690555	11	103	theme	problematic	1814:1824	arg1	aeruginosa					1798:1807	aeruginosa	1798:1807	aeruginosa	1798:1807	aeruginosa is a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis.					
32690555	5	104	theme	genes	751:755	arg1	clusters					739:746	The clusters	735:746	The clusters of genes responsible for 18 of these O antigens	735:794	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	13	105	theme	O15	2126:2128	arg1	antigens					2140:2147	the O15 and O17 O antigens	2122:2147	the O15 and O17 O antigens	2122:2147	Here, we identified and characterized the gene clusters that produce the O15 and O17 O antigens and show that they utilize a pathway for synthesis that is distinct from that of the 18 other known serotypes.					
32690555	15	106	theme	antigen	2578:2584	arg1	diversity					2586:2594	O antigen diversity	2576:2594	O antigen diversity	2576:2594	These findings expand on our understanding of O antigen biosynthesis in Gram-negative bacteria and the mechanisms that drive O antigen diversity.					
32690555	13	107	from	that	2222:2225	arg1	pathway					2178:2184	a pathway	2176:2184	a pathway for synthesis that is distinct from that of the 18 other known serotypes	2176:2257	Here, we identified and characterized the gene clusters that produce the O15 and O17 O antigens and show that they utilize a pathway for synthesis that is distinct from that of the 18 other known serotypes.					
32690555	13	107	from	that	2222:2225	arg1	distinct					2208:2215	distinct	2208:2215	distinct	2208:2215	Here, we identified and characterized the gene clusters that produce the O15 and O17 O antigens and show that they utilize a pathway for synthesis that is distinct from that of the 18 other known serotypes.					
32690555	10	108	theme	biosynthesis	1712:1723	arg1	understanding					1685:1697	a comprehensive understanding	1669:1697	a comprehensive understanding of O antigen biosynthesis in P. aeruginosa	1669:1740	The findings in this report are critical for a comprehensive understanding of O antigen biosynthesis in P. aeruginosa and provide a framework for future studies.IMPORTANCEP.					
32690555	0	109	theme	Transporter-Dependent	109:129	arg1	Pathway					141:147	an ABC Transporter-Dependent Synthesis Pathway	102:147	an ABC Transporter-Dependent Synthesis Pathway	102:147	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	5	110	from	locus	896:900	arg1	located					876:882	located	876:882	located	876:882	The clusters of genes responsible for 18 of these O antigens have been identified, all of which follow the Wzx/Wzy-dependent pathway and are located at a common locus.					
32690555	3	111	dep	structure	519:527	arg1	The					506:508	The	506:508	The	506:508	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	6	112	theme	whole-genome	1073:1084	arg1	data					1095:1098	published whole-genome sequence data	1063:1098	published whole-genome sequence data	1063:1098	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	8	113	theme	O	1359:1359	arg1	antigens					1361:1368	these O antigens	1353:1368	these O antigens	1353:1368	Based on data from knockout and overexpression studies, we determined that the synthesis of these O antigens follows an ABC transporter-dependent rather than a Wzx/Wzy-dependent pathway.					
32690555	9	114	theme	distinct	1580:1587	arg1	architectures					1609:1621	distinct and variable domain architectures	1580:1621	distinct and variable domain architectures	1580:1621	In addition, we collected evidence to show that the O15 and O17 polysaccharide chain lengths are regulated by molecular rulers with distinct and variable domain architectures.					
32690555	11	115	theme	cystic	1940:1945	arg1	fibrosis					1947:1954	cystic fibrosis	1940:1954	cystic fibrosis	1940:1954	aeruginosa is a problematic opportunistic pathogen that causes diseases in those with compromised host defenses, such as those suffering from cystic fibrosis.					
32690555	0	116	theme	Pseudomonas	22:32	arg1	O17					45:47	Pseudomonas aeruginosa O17	22:47	Pseudomonas aeruginosa O17	22:47	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	13	117	theme	other	2237:2241	arg1	serotypes					2249:2257	the 18 other known serotypes	2230:2257	the 18 other known serotypes	2230:2257	Here, we identified and characterized the gene clusters that produce the O15 and O17 O antigens and show that they utilize a pathway for synthesis that is distinct from that of the 18 other known serotypes.					
32690555	9	118	theme	variable	1593:1600	arg1	architectures					1609:1621	distinct and variable domain architectures	1580:1621	distinct and variable domain architectures	1580:1621	In addition, we collected evidence to show that the O15 and O17 polysaccharide chain lengths are regulated by molecular rulers with distinct and variable domain architectures.					
32690555	6	119	theme	structures	1039:1048	arg1	synthesis					1002:1010	the synthesis	998:1010	the synthesis of the O15 and the O17 OSA structures	998:1048	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	10	120	theme	O	1702:1702	arg1	biosynthesis					1712:1723	O antigen biosynthesis	1702:1723	O antigen biosynthesis in P. aeruginosa	1702:1740	The findings in this report are critical for a comprehensive understanding of O antigen biosynthesis in P. aeruginosa and provide a framework for future studies.IMPORTANCEP.					
32690555	0	121	theme	O17	45:47	arg1	Loci					89:92	the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci	18:92	the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci	18:92	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	6	122	theme	O17	1031:1033	arg1	structures					1039:1048	the O17 OSA structures	1027:1048	the O17 OSA structures	1027:1048	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	0	123	theme	O15	53:55	arg1	Biosynthesis					76:87	O15 O-Specific Antigen Biosynthesis	53:87	O15 O-Specific Antigen Biosynthesis	53:87	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	2	124	theme	important	468:476	arg1	interactions					492:503	important host-pathogen interactions	468:503	important host-pathogen interactions	468:503	O antigens are highly diverse polysaccharides that protect bacteria from their environment and engage in important host-pathogen interactions.					
32690555	6	125	from	located	921:927	arg1	study					911:915	this study	906:915	this study	906:915	In this study, we located the two unidentified O antigen biosynthesis clusters responsible for the synthesis of the O15 and the O17 OSA structures by analyzing published whole-genome sequence data.					
32690555	0	126	dep	Pseudomonas	22:32	arg1	aeruginosa					34:43	aeruginosa	34:43	aeruginosa	34:43	Identification of the Pseudomonas aeruginosa O17 and O15 O-Specific Antigen Biosynthesis Loci Reveals an ABC Transporter-Dependent Synthesis Pathway and Mechanisms of Genetic Diversity.					
32690555	3	127	theme	specific	510:517	arg1	structure					519:527	specific structure	510:527	specific structure	510:527	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	3	127	theme	specific	510:517	arg1	basis					567:571	the basis	563:571	the basis of classifying bacteria into O serotypes	563:612	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	3	127	theme	specific	510:517	arg1	composition					533:543	composition	533:543	composition	533:543	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	1	128	theme	O	235:235	arg1	component					245:253	the O antigen component	231:253	the O antigen component of lipopolysaccharide (LPS)	231:281	Many bacterial cell surface glycans, such as the O antigen component of lipopolysaccharide (LPS), are produced via the so-called Wzx/Wzy- or ABC transporter-dependent pathways.					
32690555	10	129	from	understanding	1685:1697	arg1	aeruginosa					1731:1740	P. aeruginosa	1728:1740	P. aeruginosa	1728:1740	The findings in this report are critical for a comprehensive understanding of O antigen biosynthesis in P. aeruginosa and provide a framework for future studies.IMPORTANCEP.					
32690555	3	130	theme	antigens	550:557	arg1	structure					519:527	specific structure	510:527	specific structure	510:527	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	3	130	theme	antigens	550:557	arg1	basis					567:571	the basis	563:571	the basis of classifying bacteria into O serotypes	563:612	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
32690555	3	130	theme	antigens	550:557	arg1	composition					533:543	composition	533:543	composition	533:543	The specific structure and composition of O antigens are the basis of classifying bacteria into O serotypes.					
33463935	1	0	dep	lucidum	303:309	arg1	WG					312:313	WG	312:313	WG	312:313	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	1	0	dep	lucidum	303:309	arg1	SG					319:320	SG	319:320	SG	319:320	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	4	1	theme	extracts	727:734	arg1	activities					706:715	activities	706:715	activities of the WG extracts	706:734	The phenolic extracts exhibited higher antioxidant and hypoglycemic activity than the polysaccharidic extracts, and activities of the WG extracts were better than those of the SG extracts.					
33463935	2	2	theme	proximate	342:350	arg1	composition					352:362	proximate composition	342:362	proximate composition	342:362	The proximate composition, microelement content, and functional compounds of the two different cultivated mushrooms were investigated.					
33463935	1	3	theme	biological	236:245	arg1	activities					247:256	biological activities	236:256	biological activities	236:256	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	5	4	theme	wood	790:793	arg1	culture					795:801	wood culture	790:801	wood culture	790:801	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	5	4	theme	wood	790:793	arg1	method					817:822	a superior method	806:822	a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities	806:915	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	4	5	theme	WG	724:725	arg1	extracts					727:734	the WG extracts	720:734	the WG extracts	720:734	The phenolic extracts exhibited higher antioxidant and hypoglycemic activity than the polysaccharidic extracts, and activities of the WG extracts were better than those of the SG extracts.					
33463935	0	6	from	Effect	0:5	arg1	Activities					73:82	Biological Activities	62:82	Biological Activities	62:82	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	0	6	from	Effect	0:5	arg1	Composition					46:56	Chemical Composition	37:56	Chemical Composition	37:56	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	0	7	theme	Lingzhi	87:93	arg1	Activities					73:82	Biological Activities	62:82	Biological Activities	62:82	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	0	7	theme	Lingzhi	87:93	arg1	Composition					46:56	Chemical Composition	37:56	Chemical Composition	37:56	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	3	8	theme	SG	563:564	arg1	polysaccharide					516:529	polysaccharide	516:529	polysaccharide	516:529	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG were also investigated.					
33463935	3	8	theme	SG	563:564	arg1	extracts					544:551	phenolic extracts	535:551	phenolic extracts of WG and SG	535:564	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG were also investigated.					
33463935	3	9	theme	polysaccharide	516:529	arg1	activities					502:511	Antioxidant and hypoglycemic activities	473:511	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG	473:564	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG were also investigated.					
33463935	1	10	theme	wood-cultured	261:273	arg1	lucidum					303:309	Ganoderma lucidum	293:309	wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively)	261:335	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	0	11	theme	Medicinal	105:113	arg1	Mushroom					115:122	Medicinal Mushroom	105:122	Medicinal Mushroom	105:122	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	5	12	theme	such	872:875	arg1	activities					906:915	such antioxidant and hypoglycemic activities	872:915	such antioxidant and hypoglycemic activities	872:915	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	0	13	theme	Reishi	98:103	arg1	Activities					73:82	Biological Activities	62:82	Biological Activities	62:82	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	0	13	theme	Reishi	98:103	arg1	Composition					46:56	Chemical Composition	37:56	Chemical Composition	37:56	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	4	14	theme	phenolic	594:601	arg1	extracts					603:610	The phenolic extracts	590:610	The phenolic extracts	590:610	The phenolic extracts exhibited higher antioxidant and hypoglycemic activity than the polysaccharidic extracts, and activities of the WG extracts were better than those of the SG extracts.					
33463935	0	15	dep	Lingzhi	87:93	arg1	Mushroom					115:122	Medicinal Mushroom	105:122	Medicinal Mushroom	105:122	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	1	16	theme	systematic	163:172	arg1	study					174:178	A systematic study	161:178	A systematic study	161:178	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	2	17	theme	mushrooms	444:452	arg1	compounds					402:410	functional compounds	391:410	functional compounds	391:410	The proximate composition, microelement content, and functional compounds of the two different cultivated mushrooms were investigated.					
33463935	2	17	theme	mushrooms	444:452	arg1	composition					352:362	proximate composition	342:362	proximate composition	342:362	The proximate composition, microelement content, and functional compounds of the two different cultivated mushrooms were investigated.					
33463935	2	17	theme	mushrooms	444:452	arg1	content					378:384	microelement content	365:384	microelement content	365:384	The proximate composition, microelement content, and functional compounds of the two different cultivated mushrooms were investigated.					
33463935	2	18	theme	cultivated	433:442	arg1	mushrooms					444:452	the two different cultivated mushrooms	415:452	the two different cultivated mushrooms	415:452	The proximate composition, microelement content, and functional compounds of the two different cultivated mushrooms were investigated.					
33463935	2	19	theme	microelement	365:376	arg1	content					378:384	microelement content	365:384	microelement content	365:384	The proximate composition, microelement content, and functional compounds of the two different cultivated mushrooms were investigated.					
33463935	0	20	theme	Substrates	19:28	arg1	Effect					0:5	Effect	0:5	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom	0:122	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	0	20	theme	Substrates	19:28	arg1	lucidum					135:141	Ganoderma lucidum	125:141	Ganoderma lucidum (Agaricomycetes)	125:158	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	1	21	theme	sack-cultured	279:291	arg1	lucidum					303:309	Ganoderma lucidum	293:309	wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively)	261:335	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	0	22	theme	Cultured	10:17	arg1	Substrates					19:28	Cultured Substrates	10:28	Cultured Substrates	10:28	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	1	23	theme	Ganoderma	293:301	arg1	lucidum					303:309	Ganoderma lucidum	293:309	wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively)	261:335	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	1	24	dep	composition	220:230	arg1	the					207:209	the	207:209	the	207:209	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	4	25	theme	SG	766:767	arg1	extracts					769:776	the SG extracts	762:776	the SG extracts	762:776	The phenolic extracts exhibited higher antioxidant and hypoglycemic activity than the polysaccharidic extracts, and activities of the WG extracts were better than those of the SG extracts.					
33463935	3	26	theme	Antioxidant	473:483	arg1	activities					502:511	Antioxidant and hypoglycemic activities	473:511	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG	473:564	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG were also investigated.					
33463935	1	27	theme	lucidum	303:309	arg1	composition					220:230	chemical composition	211:230	chemical composition	211:230	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	1	27	theme	lucidum	303:309	arg1	activities					247:256	biological activities	236:256	biological activities	236:256	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	3	28	theme	WG	556:557	arg1	polysaccharide					516:529	polysaccharide	516:529	polysaccharide	516:529	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG were also investigated.					
33463935	3	28	theme	WG	556:557	arg1	extracts					544:551	phenolic extracts	535:551	phenolic extracts of WG and SG	535:564	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG were also investigated.					
33463935	0	29	dep	Composition	46:56	arg1	the					33:35	the	33:35	the	33:35	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	5	30	theme	hypoglycemic	893:904	arg1	activities					906:915	such antioxidant and hypoglycemic activities	872:915	such antioxidant and hypoglycemic activities	872:915	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	4	31	theme	polysaccharidic	676:690	arg1	extracts					692:699	the polysaccharidic extracts	672:699	the polysaccharidic extracts	672:699	The phenolic extracts exhibited higher antioxidant and hypoglycemic activity than the polysaccharidic extracts, and activities of the WG extracts were better than those of the SG extracts.					
33463935	0	32	theme	Ganoderma	125:133	arg1	Agaricomycetes					144:157	Agaricomycetes	144:157	Agaricomycetes	144:157	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	0	32	theme	Ganoderma	125:133	arg1	Effect					0:5	Effect	0:5	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom	0:122	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	0	32	theme	Ganoderma	125:133	arg1	lucidum					135:141	Ganoderma lucidum	125:141	Ganoderma lucidum (Agaricomycetes)	125:158	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	2	33	theme	different	423:431	arg1	mushrooms					444:452	the two different cultivated mushrooms	415:452	the two different cultivated mushrooms	415:452	The proximate composition, microelement content, and functional compounds of the two different cultivated mushrooms were investigated.					
33463935	2	34	theme	functional	391:400	arg1	compounds					402:410	functional compounds	391:410	functional compounds	391:410	The proximate composition, microelement content, and functional compounds of the two different cultivated mushrooms were investigated.					
33463935	5	35	theme	activities	906:915	arg1	production					858:867	the production	854:867	the production of such antioxidant and hypoglycemic activities	854:915	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	5	36	theme	superior	808:815	arg1	culture					795:801	wood culture	790:801	wood culture	790:801	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	5	36	theme	superior	808:815	arg1	method					817:822	a superior method	806:822	a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities	806:915	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	0	37	theme	Chemical	37:44	arg1	Composition					46:56	Chemical Composition	37:56	Chemical Composition	37:56	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	5	38	theme	artificial	827:836	arg1	cultivation					838:848	artificial cultivation	827:848	artificial cultivation	827:848	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	2	39	dep	composition	352:362	arg1	The					338:340	The	338:340	The	338:340	The proximate composition, microelement content, and functional compounds of the two different cultivated mushrooms were investigated.					
33463935	3	40	theme	hypoglycemic	489:500	arg1	activities					502:511	Antioxidant and hypoglycemic activities	473:511	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG	473:564	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG were also investigated.					
33463935	1	41	theme	chemical	211:218	arg1	composition					220:230	chemical composition	211:230	chemical composition	211:230	A systematic study was carried out to compare the chemical composition and biological activities of wood-cultured and sack-cultured Ganoderma lucidum (WG and SG, respectively).					
33463935	0	42	theme	Biological	62:71	arg1	Activities					73:82	Biological Activities	62:82	Biological Activities	62:82	Effect of Cultured Substrates on the Chemical Composition and Biological Activities of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).					
33463935	4	43	dep	higher	622:627	arg1	antioxidant					629:639	antioxidant	629:639	antioxidant	629:639	The phenolic extracts exhibited higher antioxidant and hypoglycemic activity than the polysaccharidic extracts, and activities of the WG extracts were better than those of the SG extracts.					
33463935	4	43	dep	higher	622:627	arg1	hypoglycemic					645:656	hypoglycemic	645:656	hypoglycemic	645:656	The phenolic extracts exhibited higher antioxidant and hypoglycemic activity than the polysaccharidic extracts, and activities of the WG extracts were better than those of the SG extracts.					
33463935	3	44	theme	phenolic	535:542	arg1	extracts					544:551	phenolic extracts	535:551	phenolic extracts of WG and SG	535:564	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG were also investigated.					
33463935	5	45	theme	antioxidant	877:887	arg1	activities					906:915	such antioxidant and hypoglycemic activities	872:915	such antioxidant and hypoglycemic activities	872:915	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	5	46	theme	cultivation	838:848	arg1	culture					795:801	wood culture	790:801	wood culture	790:801	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	5	46	theme	cultivation	838:848	arg1	method					817:822	a superior method	806:822	a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities	806:915	Therefore, wood culture is a superior method of artificial cultivation for the production of such antioxidant and hypoglycemic activities.					
33463935	4	47	theme	higher	622:627	arg1	activity					658:665	higher antioxidant and hypoglycemic activity	622:665	higher antioxidant and hypoglycemic activity than the polysaccharidic extracts	622:699	The phenolic extracts exhibited higher antioxidant and hypoglycemic activity than the polysaccharidic extracts, and activities of the WG extracts were better than those of the SG extracts.					
33463935	3	48	theme	extracts	544:551	arg1	activities					502:511	Antioxidant and hypoglycemic activities	473:511	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG	473:564	Antioxidant and hypoglycemic activities of polysaccharide and phenolic extracts of WG and SG were also investigated.					
34159784	4	0	theme	acid	683:686	arg1	operon					688:693	the colanic acid operon	671:693	the colanic acid operon	671:693	The accumulation of each enzyme product was made possible by inactivating sequential genes involved in colanic acid biosynthesis and upregulating the colanic acid operon by inducing rcsA transcription.					
34159784	2	1	theme	colanic	348:354	arg1	acid					356:359	colanic acid	348:359	colanic acid	348:359	For nearly six decades since its discovery, the functional identities of the enzymes necessary to synthesize colanic acid have yet to be assessed in full.					
34159784	4	2	theme	enzyme	550:555	arg1	product					557:563	each enzyme product	545:563	each enzyme product	545:563	The accumulation of each enzyme product was made possible by inactivating sequential genes involved in colanic acid biosynthesis and upregulating the colanic acid operon by inducing rcsA transcription.					
34159784	5	3	theme	accumulated	762:772	arg1	materials					774:782	these accumulated materials	756:782	these accumulated materials	756:782	LC-MS analysis revealed that these accumulated materials were consistent with the well-documented composition analysis.					
34159784	6	4	theme	functional	928:937	arg1	roles					939:943	the functional roles	924:943	the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid	924:1080	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	3	5	from	step	479:482	arg1	intermediates					455:467	the lipid-linked intermediates	438:467	the lipid-linked intermediates from each step of colanic acid biosynthesis	438:511	Herein, we developed a method for detecting the lipid-linked intermediates from each step of colanic acid biosynthesis in E. coli.					
34159784	1	6	theme	outer	164:168	arg1	membrane					170:177	the outer membrane	160:177	the outer membrane of Escherichia coli	160:197	Colanic acid is a glycopolymer loosely associated with the outer membrane of Escherichia coli that plays a role in pathogen survival.					
34159784	5	7	with	consistent	789:798	arg1	analysis					837:844	the well-documented composition analysis	805:844	the well-documented composition analysis	805:844	LC-MS analysis revealed that these accumulated materials were consistent with the well-documented composition analysis.					
34159784	8	8	theme	functions	1392:1400	arg1	identification					1367:1380	the identification	1363:1380	the identification of enzyme functions during cell envelope synthesis	1363:1431	These findings provide insight into the development of methods for the identification of enzyme functions during cell envelope synthesis.					
34159784	8	9	theme	methods	1351:1357	arg1	development					1336:1346	the development	1332:1346	the development of methods for the identification of enzyme functions during cell envelope synthesis	1332:1431	These findings provide insight into the development of methods for the identification of enzyme functions during cell envelope synthesis.					
34159784	4	10	theme	rcsA	707:710	arg1	transcription					712:724	rcsA transcription	707:724	rcsA transcription	707:724	The accumulation of each enzyme product was made possible by inactivating sequential genes involved in colanic acid biosynthesis and upregulating the colanic acid operon by inducing rcsA transcription.					
34159784	4	11	theme	product	557:563	arg1	accumulation					529:540	The accumulation	525:540	The accumulation of each enzyme product	525:563	The accumulation of each enzyme product was made possible by inactivating sequential genes involved in colanic acid biosynthesis and upregulating the colanic acid operon by inducing rcsA transcription.					
34159784	5	12	theme	LC-MS	727:731	arg1	analysis					733:740	LC-MS analysis	727:740	LC-MS analysis	727:740	LC-MS analysis revealed that these accumulated materials were consistent with the well-documented composition analysis.					
34159784	3	13	theme	biosynthesis	500:511	arg1	step					479:482	each step	474:482	each step of colanic acid biosynthesis	474:511	Herein, we developed a method for detecting the lipid-linked intermediates from each step of colanic acid biosynthesis in E. coli.					
34159784	4	14	theme	acid	636:639	arg1	biosynthesis					641:652	colanic acid biosynthesis	628:652	colanic acid biosynthesis	628:652	The accumulation of each enzyme product was made possible by inactivating sequential genes involved in colanic acid biosynthesis and upregulating the colanic acid operon by inducing rcsA transcription.					
34159784	5	15	theme	well-documented	809:823	arg1	analysis					837:844	the well-documented composition analysis	805:844	the well-documented composition analysis	805:844	LC-MS analysis revealed that these accumulated materials were consistent with the well-documented composition analysis.					
34159784	4	16	theme	colanic	628:634	arg1	biosynthesis					641:652	colanic acid biosynthesis	628:652	colanic acid biosynthesis	628:652	The accumulation of each enzyme product was made possible by inactivating sequential genes involved in colanic acid biosynthesis and upregulating the colanic acid operon by inducing rcsA transcription.					
34159784	0	17	theme	Colanic	9:15	arg1	Unit					29:32	Tracking Colanic Acid Repeat Unit	0:32	Tracking Colanic Acid Repeat Unit	0:32	Tracking Colanic Acid Repeat Unit Formation from Stepwise Biosynthesis Inactivation in Escherichia coli.					
34159784	8	18	theme	enzyme	1385:1390	arg1	functions					1392:1400	enzyme functions	1385:1400	enzyme functions	1385:1400	These findings provide insight into the development of methods for the identification of enzyme functions during cell envelope synthesis.					
34159784	6	19	theme	acid	1077:1080	arg1	unit					1061:1064	unit	1061:1064	unit of colanic acid	1061:1080	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	0	20	theme	Tracking	0:7	arg1	Unit					29:32	Tracking Colanic Acid Repeat Unit	0:32	Tracking Colanic Acid Repeat Unit	0:32	Tracking Colanic Acid Repeat Unit Formation from Stepwise Biosynthesis Inactivation in Escherichia coli.					
34159784	7	21	theme	biochemical	1096:1106	arg1	evidence					1108:1115	biochemical evidence	1096:1115	biochemical evidence	1096:1115	Importantly, biochemical evidence is provided for the formation of the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK.					
34159784	1	22	theme	coli	194:197	arg1	membrane					170:177	the outer membrane	160:177	the outer membrane of Escherichia coli	160:197	Colanic acid is a glycopolymer loosely associated with the outer membrane of Escherichia coli that plays a role in pathogen survival.					
34159784	0	23	theme	Repeat	22:27	arg1	Unit					29:32	Tracking Colanic Acid Repeat Unit	0:32	Tracking Colanic Acid Repeat Unit	0:32	Tracking Colanic Acid Repeat Unit Formation from Stepwise Biosynthesis Inactivation in Escherichia coli.					
34159784	2	24	theme	functional	287:296	arg1	identities					298:307	the functional identities	283:307	the functional identities of the enzymes necessary to synthesize colanic acid	283:359	For nearly six decades since its discovery, the functional identities of the enzymes necessary to synthesize colanic acid have yet to be assessed in full.					
34159784	0	25	theme	Acid	17:20	arg1	Unit					29:32	Tracking Colanic Acid Repeat Unit	0:32	Tracking Colanic Acid Repeat Unit	0:32	Tracking Colanic Acid Repeat Unit Formation from Stepwise Biosynthesis Inactivation in Escherichia coli.					
34159784	6	26	link	lipid-linked	1022:1033	arg1	oligosaccharide					1035:1049	the lipid-linked oligosaccharide	1018:1049	the lipid-linked oligosaccharide repeating unit of colanic acid	1018:1080	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	6	27	theme	acid	896:899	arg1	bioassembly					873:883	the native bioassembly	862:883	the native bioassembly of colanic acid	862:899	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	4	28	theme	colanic	675:681	arg1	operon					688:693	the colanic acid operon	671:693	the colanic acid operon	671:693	The accumulation of each enzyme product was made possible by inactivating sequential genes involved in colanic acid biosynthesis and upregulating the colanic acid operon by inducing rcsA transcription.					
34159784	4	29	theme	sequential	599:608	arg1	genes					610:614	sequential genes	599:614	sequential genes involved in colanic acid biosynthesis	599:652	The accumulation of each enzyme product was made possible by inactivating sequential genes involved in colanic acid biosynthesis and upregulating the colanic acid operon by inducing rcsA transcription.					
34159784	7	30	theme	final	1154:1158	arg1	product					1189:1195	the final glycosylation hexasaccharide product	1150:1195	the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK	1150:1293	Importantly, biochemical evidence is provided for the formation of the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK.					
34159784	6	31	theme	colanic	888:894	arg1	acid					896:899	colanic acid	888:899	colanic acid	888:899	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	8	32	theme	envelope	1414:1421	arg1	synthesis					1423:1431	cell envelope synthesis	1409:1431	cell envelope synthesis	1409:1431	These findings provide insight into the development of methods for the identification of enzyme functions during cell envelope synthesis.					
34159784	6	33	theme	colanic	1069:1075	arg1	acid					1077:1080	colanic acid	1069:1080	colanic acid	1069:1080	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	7	34	theme	glycosylation	1160:1172	arg1	product					1189:1195	the final glycosylation hexasaccharide product	1150:1195	the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK	1150:1293	Importantly, biochemical evidence is provided for the formation of the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK.					
34159784	6	35	theme	enzymes	961:967	arg1	roles					939:943	the functional roles	924:943	the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid	924:1080	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	0	36	from	Inactivation	71:82	arg1	Formation					34:42	Formation	34:42	Formation	34:42	Tracking Colanic Acid Repeat Unit Formation from Stepwise Biosynthesis Inactivation in Escherichia coli.					
34159784	0	36	from	Inactivation	71:82	arg1	coli					99:102	Escherichia coli	87:102	Escherichia coli	87:102	Tracking Colanic Acid Repeat Unit Formation from Stepwise Biosynthesis Inactivation in Escherichia coli.					
34159784	5	37	theme	composition	825:835	arg1	analysis					837:844	the well-documented composition analysis	805:844	the well-documented composition analysis	805:844	LC-MS analysis revealed that these accumulated materials were consistent with the well-documented composition analysis.					
34159784	6	38	theme	oligosaccharide	1035:1049	arg1	formation					1005:1013	the formation	1001:1013	the formation of the lipid-linked oligosaccharide repeating unit of colanic acid	1001:1080	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	0	39	theme	Stepwise	49:56	arg1	Inactivation					71:82	Stepwise Biosynthesis Inactivation	49:82	Stepwise Biosynthesis Inactivation in Escherichia coli	49:102	Tracking Colanic Acid Repeat Unit Formation from Stepwise Biosynthesis Inactivation in Escherichia coli.					
34159784	3	40	link	lipid-linked	442:453	arg1	intermediates					455:467	the lipid-linked intermediates	438:467	the lipid-linked intermediates from each step of colanic acid biosynthesis	438:511	Herein, we developed a method for detecting the lipid-linked intermediates from each step of colanic acid biosynthesis in E. coli.					
34159784	7	41	theme	moiety	1243:1248	arg1	WcaL					1207:1210	WcaL	1207:1210	WcaL	1207:1210	Importantly, biochemical evidence is provided for the formation of the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK.					
34159784	7	41	theme	moiety	1243:1248	arg1	addition					1220:1227	the addition	1216:1227	the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK	1216:1293	Importantly, biochemical evidence is provided for the formation of the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK.					
34159784	6	42	theme	last	952:955	arg1	WcaL					970:973	WcaL	970:973	WcaL	970:973	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	6	42	theme	last	952:955	arg1	enzymes					961:967	the last two enzymes	948:967	the last two enzymes	948:967	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	6	42	theme	last	952:955	arg1	WcaK					979:982	WcaK	979:982	WcaK	979:982	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	3	43	theme	lipid-linked	442:453	arg1	intermediates					455:467	the lipid-linked intermediates	438:467	the lipid-linked intermediates from each step of colanic acid biosynthesis	438:511	Herein, we developed a method for detecting the lipid-linked intermediates from each step of colanic acid biosynthesis in E. coli.					
34159784	6	44	theme	native	866:871	arg1	bioassembly					873:883	the native bioassembly	862:883	the native bioassembly of colanic acid	862:899	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	2	45	theme	necessary	324:332	arg1	enzymes					316:322	the enzymes	312:322	the enzymes necessary to synthesize colanic acid	312:359	For nearly six decades since its discovery, the functional identities of the enzymes necessary to synthesize colanic acid have yet to be assessed in full.					
34159784	7	46	theme	hexasaccharide	1174:1187	arg1	product					1189:1195	the final glycosylation hexasaccharide product	1150:1195	the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK	1150:1293	Importantly, biochemical evidence is provided for the formation of the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK.					
34159784	8	47	theme	cell	1409:1412	arg1	synthesis					1423:1431	cell envelope synthesis	1409:1431	cell envelope synthesis	1409:1431	These findings provide insight into the development of methods for the identification of enzyme functions during cell envelope synthesis.					
34159784	2	48	theme	enzymes	316:322	arg1	identities					298:307	the functional identities	283:307	the functional identities of the enzymes necessary to synthesize colanic acid	283:359	For nearly six decades since its discovery, the functional identities of the enzymes necessary to synthesize colanic acid have yet to be assessed in full.					
34159784	1	49	theme	Colanic	105:111	arg1	glycopolymer					123:134	a glycopolymer	121:134	a glycopolymer loosely associated with the outer membrane of Escherichia coli that plays a role in pathogen survival	121:236	Colanic acid is a glycopolymer loosely associated with the outer membrane of Escherichia coli that plays a role in pathogen survival.					
34159784	1	49	theme	Colanic	105:111	arg1	acid					113:116	Colanic acid	105:116	Colanic acid	105:116	Colanic acid is a glycopolymer loosely associated with the outer membrane of Escherichia coli that plays a role in pathogen survival.					
34159784	6	50	theme	lipid-linked	1022:1033	arg1	oligosaccharide					1035:1049	the lipid-linked oligosaccharide	1018:1049	the lipid-linked oligosaccharide repeating unit of colanic acid	1018:1080	Recapitulating the native bioassembly of colanic acid enabled us to identify the functional roles of the last two enzymes, WcaL and WcaK, associated with the formation of the lipid-linked oligosaccharide repeating unit of colanic acid.					
34159784	7	51	theme	pyruvylated	1260:1270	arg1	hexasaccharide					1272:1285	a pyruvylated hexasaccharide	1258:1285	a pyruvylated hexasaccharide by WcaK	1258:1293	Importantly, biochemical evidence is provided for the formation of the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK.					
34159784	1	52	theme	pathogen	220:227	arg1	survival					229:236	pathogen survival	220:236	pathogen survival	220:236	Colanic acid is a glycopolymer loosely associated with the outer membrane of Escherichia coli that plays a role in pathogen survival.					
34159784	7	53	theme	pyruvate	1234:1241	arg1	moiety					1243:1248	a pyruvate moiety	1232:1248	a pyruvate moiety	1232:1248	Importantly, biochemical evidence is provided for the formation of the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK.					
34159784	3	54	theme	colanic	487:493	arg1	biosynthesis					500:511	colanic acid biosynthesis	487:511	colanic acid biosynthesis	487:511	Herein, we developed a method for detecting the lipid-linked intermediates from each step of colanic acid biosynthesis in E. coli.					
34159784	0	55	theme	Biosynthesis	58:69	arg1	Inactivation					71:82	Stepwise Biosynthesis Inactivation	49:82	Stepwise Biosynthesis Inactivation in Escherichia coli	49:102	Tracking Colanic Acid Repeat Unit Formation from Stepwise Biosynthesis Inactivation in Escherichia coli.					
34159784	7	56	theme	product	1189:1195	arg1	formation					1137:1145	the formation	1133:1145	the formation of the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK	1133:1293	Importantly, biochemical evidence is provided for the formation of the final glycosylation hexasaccharide product formed by WcaL and the addition of a pyruvate moiety to form a pyruvylated hexasaccharide by WcaK.					
34159784	3	57	theme	acid	495:498	arg1	biosynthesis					500:511	colanic acid biosynthesis	487:511	colanic acid biosynthesis	487:511	Herein, we developed a method for detecting the lipid-linked intermediates from each step of colanic acid biosynthesis in E. coli.					
33858566	7	0	theme	high	1108:1111	arg1	concentration					1120:1132	high enzyme concentration	1108:1132	high enzyme concentration	1108:1132	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	1	1	theme	agro-industrial	136:150	arg1	reutilisation					158:170	agro-industrial waste reutilisation	136:170	agro-industrial waste reutilisation	136:170	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	7	2	theme	xylan	945:949	arg1	conversions					951:961	High xylan conversions	940:961	High xylan conversions	940:961	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	4	3	theme	recovery	514:521	arg1	conditions					523:532	optimal recovery conditions	506:532	optimal recovery conditions	506:532	At optimal recovery conditions, the fractions were further hydrolysed to XOS using GH11 endo-xylanase, by varying time and enzyme concentration.					
33858566	5	4	theme	obtained	707:714	arg1	hemicelluloses					716:729	the obtained hemicelluloses	703:729	the obtained hemicelluloses	703:729	Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well.					
33858566	7	5	theme	h	984:984	arg1	hydrolysis					986:995	24 h hydrolysis	981:995	24 h hydrolysis	981:995	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	1	6	theme	waste	152:156	arg1	reutilisation					158:170	agro-industrial waste reutilisation	136:170	agro-industrial waste reutilisation	136:170	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	4	7	theme	optimal	506:512	arg1	conditions					523:532	optimal recovery conditions	506:532	optimal recovery conditions	506:532	At optimal recovery conditions, the fractions were further hydrolysed to XOS using GH11 endo-xylanase, by varying time and enzyme concentration.					
33858566	2	8	theme	paper	289:293	arg1	aim					277:279	The aim	273:279	The aim of this paper	273:293	The aim of this paper was to evaluate WS and PP as sources for the production of xylooligosaccharides (XOS).					
33858566	3	9	theme	delignification	462:476	arg1	parameters					448:457	varying parameters	440:457	varying parameters of delignification and alkaline extraction	440:500	Hemicelluloses from WS and PP were recovered by combining varying parameters of delignification and alkaline extraction.					
33858566	1	10	theme	pea	244:246	arg1	PP					253:254	PP	253:254	PP	253:254	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	1	10	theme	pea	244:246	arg1	pod					248:250	pea pod	244:250	pea pod (PP)	244:255	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	1	11	from	interest	124:131	arg1	reutilisation					158:170	agro-industrial waste reutilisation	136:170	agro-industrial waste reutilisation	136:170	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	4	12	theme	enzyme	626:631	arg1	concentration					633:645	enzyme concentration	626:645	enzyme concentration	626:645	At optimal recovery conditions, the fractions were further hydrolysed to XOS using GH11 endo-xylanase, by varying time and enzyme concentration.					
33858566	5	13	dep	building	732:739	arg1	PP					807:808	PP	807:808	PP	807:808	Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well.					
33858566	0	14	theme	xylooligosaccharides	80:99	arg1	production					66:75	the production	62:75	the production of xylooligosaccharides	62:99	Valorisation of walnut shell and pea pod as novel sources for the production of xylooligosaccharides.					
33858566	5	15	theme	hemicelluloses	716:729	arg1	composition					688:698	the monomeric composition	674:698	the monomeric composition of the obtained hemicelluloses	674:729	Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well.					
33858566	3	16	from	WS	402:403	arg1	Hemicelluloses					382:395	Hemicelluloses	382:395	Hemicelluloses from WS and PP	382:410	Hemicelluloses from WS and PP were recovered by combining varying parameters of delignification and alkaline extraction.					
33858566	4	17	theme	GH11	586:589	arg1	endo-xylanase					591:603	GH11 endo-xylanase	586:603	GH11 endo-xylanase	586:603	At optimal recovery conditions, the fractions were further hydrolysed to XOS using GH11 endo-xylanase, by varying time and enzyme concentration.					
33858566	6	18	dep	70	934:935	arg1	to					922:923	to	922:923	to	922:923	Delignification was essential for high recovery of total xylose from the materials, up to at least 70 %.					
33858566	7	19	theme	enzyme	1113:1118	arg1	concentration					1120:1132	high enzyme concentration	1108:1132	high enzyme concentration	1108:1132	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	1	20	theme	underutilised	173:185	arg1	shell					229:233	walnut shell	222:233	walnut shell (WS)	222:238	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	1	20	theme	underutilised	173:185	arg1	materials					203:211	underutilised lignocellulosic materials	173:211	underutilised lignocellulosic materials	173:211	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	1	20	theme	underutilised	173:185	arg1	pod					248:250	pea pod	244:250	pea pod (PP)	244:255	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	5	21	theme	arabino	755:761	arg1	glucuronoxylan					763:776	low-branched (arabino)glucuronoxylan	741:776	low-branched (arabino)glucuronoxylan	741:776	Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well.					
33858566	7	22	theme	low	1047:1049	arg1	concentration					1058:1070	low enzyme concentration	1047:1070	low enzyme concentration	1047:1070	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	3	23	from	PP	409:410	arg1	Hemicelluloses					382:395	Hemicelluloses	382:395	Hemicelluloses from WS and PP	382:410	Hemicelluloses from WS and PP were recovered by combining varying parameters of delignification and alkaline extraction.					
33858566	1	24	theme	lignocellulosic	187:201	arg1	shell					229:233	walnut shell	222:233	walnut shell (WS)	222:238	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	1	24	theme	lignocellulosic	187:201	arg1	materials					203:211	underutilised lignocellulosic materials	173:211	underutilised lignocellulosic materials	173:211	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	1	24	theme	lignocellulosic	187:201	arg1	pod					248:250	pea pod	244:250	pea pod (PP)	244:255	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	0	25	theme	shell	23:27	arg1	Valorisation					0:11	Valorisation	0:11	Valorisation of walnut shell and pea pod as novel sources for the production of xylooligosaccharides.	0:100	Valorisation of walnut shell and pea pod as novel sources for the production of xylooligosaccharides.					
33858566	3	26	theme	alkaline	482:489	arg1	extraction					491:500	alkaline extraction	482:500	alkaline extraction	482:500	Hemicelluloses from WS and PP were recovered by combining varying parameters of delignification and alkaline extraction.					
33858566	0	27	theme	walnut	16:21	arg1	shell					23:27	walnut shell	16:27	walnut shell	16:27	Valorisation of walnut shell and pea pod as novel sources for the production of xylooligosaccharides.					
33858566	6	28	theme	xylose	892:897	arg1	recovery					874:881	high recovery	869:881	high recovery of total xylose from the materials	869:916	Delignification was essential for high recovery of total xylose from the materials, up to at least 70 %.					
33858566	7	29	theme	24	981:982	arg1	h					984:984	h	984:984	h	984:984	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	3	30	theme	extraction	491:500	arg1	parameters					448:457	varying parameters	440:457	varying parameters of delignification and alkaline extraction	440:500	Hemicelluloses from WS and PP were recovered by combining varying parameters of delignification and alkaline extraction.					
33858566	0	31	theme	pea	33:35	arg1	pod					37:39	pea pod	33:39	pea pod	33:39	Valorisation of walnut shell and pea pod as novel sources for the production of xylooligosaccharides.					
33858566	6	32	theme	total	886:890	arg1	xylose					892:897	total xylose	886:897	total xylose	886:897	Delignification was essential for high recovery of total xylose from the materials, up to at least 70 %.					
33858566	7	33	theme	enzyme	1051:1056	arg1	concentration					1058:1070	low enzyme concentration	1047:1070	low enzyme concentration	1047:1070	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	6	34	dep	essential	855:863	arg1	%					937:937	up to at least 70 %	919:937	up to at least 70 %	919:937	Delignification was essential for high recovery of total xylose from the materials, up to at least 70 %.					
33858566	5	35	from	predominant	659:669	arg1	composition					688:698	the monomeric composition	674:698	the monomeric composition of the obtained hemicelluloses	674:729	Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well.					
33858566	2	36	theme	xylooligosaccharides	354:373	arg1	production					340:349	the production	336:349	the production of xylooligosaccharides (XOS)	336:379	The aim of this paper was to evaluate WS and PP as sources for the production of xylooligosaccharides (XOS).					
33858566	5	37	theme	monomeric	678:686	arg1	composition					688:698	the monomeric composition	674:698	the monomeric composition of the obtained hemicelluloses	674:729	Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well.					
33858566	5	38	theme	low-branched	741:752	arg1	glucuronoxylan					763:776	low-branched (arabino)glucuronoxylan	741:776	low-branched (arabino)glucuronoxylan	741:776	Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well.					
33858566	7	39	with	xylobiose	1093:1101	arg1	concentration					1120:1132	high enzyme concentration	1108:1132	high enzyme concentration	1108:1132	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	0	40	theme	pod	37:39	arg1	Valorisation					0:11	Valorisation	0:11	Valorisation of walnut shell and pea pod as novel sources for the production of xylooligosaccharides.	0:100	Valorisation of walnut shell and pea pod as novel sources for the production of xylooligosaccharides.					
33858566	6	41	theme	high	869:872	arg1	recovery					874:881	high recovery	869:881	high recovery of total xylose from the materials	869:916	Delignification was essential for high recovery of total xylose from the materials, up to at least 70 %.					
33858566	6	42	from	materials	908:916	arg1	recovery					874:881	high recovery	869:881	high recovery of total xylose from the materials	869:916	Delignification was essential for high recovery of total xylose from the materials, up to at least 70 %.					
33858566	7	43	dep	while	1073:1077	arg1	xylobiose					1093:1101	xylobiose	1093:1101	xylobiose	1093:1101	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	7	43	dep	while	1073:1077	arg1	xylose					1082:1087	xylose	1082:1087	xylose	1082:1087	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	1	44	theme	walnut	222:227	arg1	WS					236:237	WS	236:237	WS	236:237	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	1	44	theme	walnut	222:227	arg1	shell					229:233	walnut shell	222:233	walnut shell (WS)	222:238	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	7	45	theme	High	940:943	arg1	conversions					951:961	High xylan conversions	940:961	High xylan conversions	940:961	High xylan conversions were obtained for 24 h hydrolysis, resulting in xylobiose and xylotriose when using low enzyme concentration, while in xylose and xylobiose with high enzyme concentration.					
33858566	5	46	from	composition	688:698	arg1	predominant					659:669	predominant	659:669	predominant	659:669	Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well.					
33858566	1	47	theme	high	119:122	arg1	interest					124:131	the high interest	115:131	the high interest in agro-industrial waste reutilisation	115:170	According to the high interest in agro-industrial waste reutilisation, underutilised lignocellulosic materials, such as walnut shell (WS) and pea pod (PP), come in focus.					
33858566	5	48	attach	predominant	659:669	arg2	Xylose					648:653	Xylose	648:653	Xylose	648:653	Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well.					
33858566	5	48	attach	predominant	659:669	arg1	composition					688:698	the monomeric composition	674:698	the monomeric composition of the obtained hemicelluloses	674:729	Xylose was predominant in the monomeric composition of the obtained hemicelluloses, building low-branched (arabino)glucuronoxylan, in WS exclusively, while in PP some xyloglucan as well.					
33858566	0	49	theme	novel	44:48	arg1	sources					50:56	novel sources	44:56	novel sources for the production of xylooligosaccharides	44:99	Valorisation of walnut shell and pea pod as novel sources for the production of xylooligosaccharides.					
33858566	3	50	theme	varying	440:446	arg1	parameters					448:457	varying parameters	440:457	varying parameters of delignification and alkaline extraction	440:500	Hemicelluloses from WS and PP were recovered by combining varying parameters of delignification and alkaline extraction.					
32475574	5	0	theme	monosaccharide	812:825	arg1	monomers					862:869	the three monomers	852:869	the three monomers of rhamnose, galactose, and glucose	852:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	0	theme	monosaccharide	812:825	arg1	rhamnose					874:881	rhamnose	874:881	rhamnose	874:881	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	0	theme	monosaccharide	812:825	arg1	galactose					884:892	galactose	884:892	galactose	884:892	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	0	theme	monosaccharide	812:825	arg1	glucose					899:905	glucose	899:905	glucose	899:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	0	theme	monosaccharide	812:825	arg1	composition					827:837	The major monosaccharide composition	802:837	The major monosaccharide composition of MRPs	802:845	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	8	1	theme	antioxidant	1280:1290	arg1	functions					1310:1318	their antioxidant and antibacterial functions	1274:1318	their antioxidant and antibacterial functions	1274:1318	The anticancer activity of polysaccharides extracted under optimal MAE conditions was highly associated with their antioxidant and antibacterial functions.					
32475574	7	2	theme	MCF-7	1144:1148	arg1	cells					1158:1162	breast (MCF-7) cancer cells	1136:1162	breast (MCF-7) cancer cells	1136:1162	MRP-P1 exhibited a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells.					
32475574	1	3	theme	biological	162:171	arg1	characterization					173:188	biological characterization	162:188	biological characterization	162:188	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	4	4	theme	scanning	714:721	arg1	microscopy					732:741	scanning electron microscopy	714:741	scanning electron microscopy	714:741	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	6	5	theme	antitumor	944:952	arg1	activities					954:963	The antioxidant, antimicrobial, and antitumor activities	908:963	The antioxidant, antimicrobial, and antitumor activities	908:963	The antioxidant, antimicrobial, and antitumor activities were increased in a concentration-dependent manner (1.0-10.0 mg/mL).					
32475574	7	6	theme	breast	1136:1141	arg1	cells					1158:1162	breast (MCF-7) cancer cells	1136:1162	breast (MCF-7) cancer cells	1136:1162	MRP-P1 exhibited a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells.					
32475574	2	7	theme	highest	305:311	arg1	MAE-yield					313:321	The highest MAE-yield	301:321	The highest MAE-yield (14.47%)	301:330	The highest MAE-yield (14.47%) was optimized at 457.32 W and 75 °C for 26 min.					
32475574	2	7	theme	highest	305:311	arg1	%					329:329	14.47%	324:329	14.47%	324:329	The highest MAE-yield (14.47%) was optimized at 457.32 W and 75 °C for 26 min.					
32475574	8	8	theme	anticancer	1169:1178	arg1	activity					1180:1187	The anticancer activity	1165:1187	The anticancer activity of polysaccharides extracted under optimal MAE conditions	1165:1245	The anticancer activity of polysaccharides extracted under optimal MAE conditions was highly associated with their antioxidant and antibacterial functions.					
32475574	0	9	from	roots	70:74	arg1	extraction					19:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.	0:131	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.					
32475574	4	10	theme	electron	723:730	arg1	microscopy					732:741	scanning electron microscopy	714:741	scanning electron microscopy	714:741	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	7	11	dep	in	1060:1061	arg1	vitro					1063:1067	vitro	1063:1067	vitro	1063:1067	MRP-P1 exhibited a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells.					
32475574	3	12	theme	symmetrical	505:515	arg1	peak					524:527	a single symmetrical narrow peak	496:527	a single symmetrical narrow peak	496:527	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32475574	8	13	theme	polysaccharides	1192:1206	arg1	activity					1180:1187	The anticancer activity	1165:1187	The anticancer activity of polysaccharides extracted under optimal MAE conditions	1165:1245	The anticancer activity of polysaccharides extracted under optimal MAE conditions was highly associated with their antioxidant and antibacterial functions.					
32475574	5	14	theme	galactose	884:892	arg1	monomers					862:869	the three monomers	852:869	the three monomers of rhamnose, galactose, and glucose	852:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	14	theme	galactose	884:892	arg1	rhamnose					874:881	rhamnose	874:881	rhamnose	874:881	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	14	theme	galactose	884:892	arg1	galactose					884:892	galactose	884:892	galactose	884:892	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	14	theme	galactose	884:892	arg1	glucose					899:905	glucose	899:905	glucose	899:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	14	theme	galactose	884:892	arg1	composition					827:837	The major monosaccharide composition	802:837	The major monosaccharide composition of MRPs	802:845	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	4	15	theme	MRP-P1	678:683	arg1	MRP-P1					678:683	MRP-P1	678:683	MRP-P1	678:683	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	4	15	theme	MRP-P1	678:683	arg1	polysaccharides					637:651	polysaccharides	637:651	polysaccharides	637:651	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	4	15	theme	MRP-P1	678:683	arg1	groups					668:673	functional groups	657:673	functional groups of MRP-P1	657:683	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	7	16	theme	cancer	1151:1156	arg1	cells					1158:1162	breast (MCF-7) cancer cells	1136:1162	breast (MCF-7) cancer cells	1136:1162	MRP-P1 exhibited a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells.					
32475574	6	17	theme	antimicrobial	925:937	arg1	activities					954:963	The antioxidant, antimicrobial, and antitumor activities	908:963	The antioxidant, antimicrobial, and antitumor activities	908:963	The antioxidant, antimicrobial, and antitumor activities were increased in a concentration-dependent manner (1.0-10.0 mg/mL).					
32475574	0	18	theme	Microwave-assisted	0:17	arg1	extraction					19:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.	0:131	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.					
32475574	3	19	theme	gel-filtration	453:466	arg1	chromatographies					468:483	ion-exchange and gel-filtration chromatographies	436:483	ion-exchange and gel-filtration chromatographies	436:483	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32475574	3	20	theme	molecular	576:584	arg1	weight					586:591	a molecular weight	574:591	a molecular weight of 4.87 × 104 Da	574:608	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32475574	1	21	theme	root	205:208	arg1	MRPs					227:230	MRPs	227:230	MRPs	227:230	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	1	21	theme	root	205:208	arg1	polysaccharides					210:224	marshmallow root polysaccharides	193:224	marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE)	193:285	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	3	22	theme	extracted	384:392	arg1	polysaccharides					400:414	The extracted crude polysaccharides	380:414	The extracted crude polysaccharides	380:414	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32475574	4	23	theme	Fourier-transform	747:763	arg1	spectroscopy					774:785	Fourier-transform infrared spectroscopy	747:785	Fourier-transform infrared spectroscopy	747:785	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	8	24	theme	antibacterial	1296:1308	arg1	functions					1310:1318	their antioxidant and antibacterial functions	1274:1318	their antioxidant and antibacterial functions	1274:1318	The anticancer activity of polysaccharides extracted under optimal MAE conditions was highly associated with their antioxidant and antibacterial functions.					
32475574	3	25	theme	ion-exchange	436:447	arg1	chromatographies					468:483	ion-exchange and gel-filtration chromatographies	436:483	ion-exchange and gel-filtration chromatographies	436:483	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32475574	0	26	theme	polysaccharides	33:47	arg1	extraction					19:28	Microwave-assisted extraction	0:28	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.	0:131	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.					
32475574	1	27	dep	optimization	145:156	arg1	The					133:135	The	133:135	The	133:135	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	5	28	theme	rhamnose	874:881	arg1	monomers					862:869	the three monomers	852:869	the three monomers of rhamnose, galactose, and glucose	852:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	28	theme	rhamnose	874:881	arg1	rhamnose					874:881	rhamnose	874:881	rhamnose	874:881	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	28	theme	rhamnose	874:881	arg1	galactose					884:892	galactose	884:892	galactose	884:892	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	28	theme	rhamnose	874:881	arg1	glucose					899:905	glucose	899:905	glucose	899:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	28	theme	rhamnose	874:881	arg1	composition					827:837	The major monosaccharide composition	802:837	The major monosaccharide composition of MRPs	802:845	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	6	29	theme	antioxidant	912:922	arg1	activities					954:963	The antioxidant, antimicrobial, and antitumor activities	908:963	The antioxidant, antimicrobial, and antitumor activities	908:963	The antioxidant, antimicrobial, and antitumor activities were increased in a concentration-dependent manner (1.0-10.0 mg/mL).					
32475574	4	30	theme	polysaccharides	637:651	arg1	morphology					623:632	The surface morphology	611:632	The surface morphology of polysaccharides and functional groups of MRP-P1	611:683	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	3	31	theme	Da	607:608	arg1	weight					586:591	a molecular weight	574:591	a molecular weight of 4.87 × 104 Da	574:608	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32475574	1	32	theme	polysaccharides	210:224	arg1	optimization					145:156	process optimization	137:156	process optimization	137:156	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	1	32	theme	polysaccharides	210:224	arg1	characterization					173:188	biological characterization	162:188	biological characterization	162:188	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	4	33	theme	groups	668:673	arg1	morphology					623:632	The surface morphology	611:632	The surface morphology of polysaccharides and functional groups of MRP-P1	611:683	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	5	34	theme	glucose	899:905	arg1	monomers					862:869	the three monomers	852:869	the three monomers of rhamnose, galactose, and glucose	852:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	34	theme	glucose	899:905	arg1	rhamnose					874:881	rhamnose	874:881	rhamnose	874:881	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	34	theme	glucose	899:905	arg1	galactose					884:892	galactose	884:892	galactose	884:892	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	34	theme	glucose	899:905	arg1	glucose					899:905	glucose	899:905	glucose	899:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	34	theme	glucose	899:905	arg1	composition					827:837	The major monosaccharide composition	802:837	The major monosaccharide composition of MRPs	802:845	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	35	theme	MRPs	842:845	arg1	monomers					862:869	the three monomers	852:869	the three monomers of rhamnose, galactose, and glucose	852:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	35	theme	MRPs	842:845	arg1	rhamnose					874:881	rhamnose	874:881	rhamnose	874:881	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	35	theme	MRPs	842:845	arg1	galactose					884:892	galactose	884:892	galactose	884:892	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	35	theme	MRPs	842:845	arg1	glucose					899:905	glucose	899:905	glucose	899:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	35	theme	MRPs	842:845	arg1	composition					827:837	The major monosaccharide composition	802:837	The major monosaccharide composition of MRPs	802:845	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	8	36	theme	MAE	1232:1234	arg1	conditions					1236:1245	optimal MAE conditions	1224:1245	optimal MAE conditions	1224:1245	The anticancer activity of polysaccharides extracted under optimal MAE conditions was highly associated with their antioxidant and antibacterial functions.					
32475574	7	37	theme	antiproliferative	1069:1085	arg1	activity					1087:1094	a strong in vitro antiproliferative activity	1051:1094	a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells	1051:1162	MRP-P1 exhibited a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells.					
32475574	4	38	theme	functional	657:666	arg1	MRP-P1					678:683	MRP-P1	678:683	MRP-P1	678:683	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	4	38	theme	functional	657:666	arg1	groups					668:673	functional groups	657:673	functional groups of MRP-P1	657:683	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	3	39	theme	crude	394:398	arg1	polysaccharides					400:414	The extracted crude polysaccharides	380:414	The extracted crude polysaccharides	380:414	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32475574	6	40	theme	concentration-dependent	985:1007	arg1	1.0-10.0 mg/mL					1017:1030	1.0-10.0 mg/mL	1017:1030	1.0-10.0 mg/mL	1017:1030	The antioxidant, antimicrobial, and antitumor activities were increased in a concentration-dependent manner (1.0-10.0 mg/mL).					
32475574	6	40	theme	concentration-dependent	985:1007	arg1	manner					1009:1014	a concentration-dependent manner	983:1014	a concentration-dependent manner (1.0-10.0 mg/mL)	983:1031	The antioxidant, antimicrobial, and antitumor activities were increased in a concentration-dependent manner (1.0-10.0 mg/mL).					
32475574	3	41	theme	narrow	517:522	arg1	peak					524:527	a single symmetrical narrow peak	496:527	a single symmetrical narrow peak	496:527	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32475574	0	42	theme	marshmallow	58:68	arg1	roots					70:74	the marshmallow roots	54:74	the marshmallow roots	54:74	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.					
32475574	7	43	theme	in	1060:1061	arg1	activity					1087:1094	a strong in vitro antiproliferative activity	1051:1094	a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells	1051:1162	MRP-P1 exhibited a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells.					
32475574	3	44	theme	single	498:503	arg1	peak					524:527	a single symmetrical narrow peak	496:527	a single symmetrical narrow peak	496:527	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32475574	4	45	theme	surface	615:621	arg1	morphology					623:632	The surface morphology	611:632	The surface morphology of polysaccharides and functional groups of MRP-P1	611:683	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	1	46	theme	process	137:143	arg1	optimization					145:156	process optimization	137:156	process optimization	137:156	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	4	47	theme	infrared	765:772	arg1	spectroscopy					774:785	Fourier-transform infrared spectroscopy	747:785	Fourier-transform infrared spectroscopy	747:785	The surface morphology of polysaccharides and functional groups of MRP-P1 were determined by employing scanning electron microscopy and Fourier-transform infrared spectroscopy, respectively.					
32475574	0	48	dep	extraction	19:28	arg1	structure					105:113	structure	105:113	structure	105:113	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.					
32475574	0	48	dep	extraction	19:28	arg1	bioactivity					120:130	bioactivity	120:130	bioactivity	120:130	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.					
32475574	0	48	dep	extraction	19:28	arg1	purification					91:102	purification	91:102	purification	91:102	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.					
32475574	0	48	dep	extraction	19:28	arg1	Optimization					77:88	Optimization	77:88	Optimization	77:88	Microwave-assisted extraction of polysaccharides from the marshmallow roots: Optimization, purification, structure, and bioactivity.					
32475574	7	49	theme	strong	1053:1058	arg1	activity					1087:1094	a strong in vitro antiproliferative activity	1051:1094	a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells	1051:1162	MRP-P1 exhibited a strong in vitro antiproliferative activity against lung (A549), liver (HepG2), and breast (MCF-7) cancer cells.					
32475574	1	50	theme	marshmallow	193:203	arg1	MRPs					227:230	MRPs	227:230	MRPs	227:230	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	1	50	theme	marshmallow	193:203	arg1	polysaccharides					210:224	marshmallow root polysaccharides	193:224	marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE)	193:285	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	1	51	theme	microwave-assisted	251:268	arg1	MAE					282:284	MAE	282:284	MAE	282:284	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	1	51	theme	microwave-assisted	251:268	arg1	extraction					270:279	the microwave-assisted extraction	247:279	the microwave-assisted extraction (MAE)	247:285	The process optimization and biological characterization of marshmallow root polysaccharides (MRPs) obtained from the microwave-assisted extraction (MAE) were studied.					
32475574	5	52	theme	major	806:810	arg1	monomers					862:869	the three monomers	852:869	the three monomers of rhamnose, galactose, and glucose	852:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	52	theme	major	806:810	arg1	rhamnose					874:881	rhamnose	874:881	rhamnose	874:881	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	52	theme	major	806:810	arg1	galactose					884:892	galactose	884:892	galactose	884:892	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	52	theme	major	806:810	arg1	glucose					899:905	glucose	899:905	glucose	899:905	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	5	52	theme	major	806:810	arg1	composition					827:837	The major monosaccharide composition	802:837	The major monosaccharide composition of MRPs	802:845	The major monosaccharide composition of MRPs were the three monomers of rhamnose, galactose, and glucose.					
32475574	8	53	theme	optimal	1224:1230	arg1	conditions					1236:1245	optimal MAE conditions	1224:1245	optimal MAE conditions	1224:1245	The anticancer activity of polysaccharides extracted under optimal MAE conditions was highly associated with their antioxidant and antibacterial functions.					
32475574	3	54	theme	homogenous	540:549	arg1	MRP-P1					561:566	MRP-P1	561:566	MRP-P1	561:566	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32475574	3	54	theme	homogenous	540:549	arg1	fraction					551:558	a homogenous fraction	538:558	a homogenous fraction (MRP-P1)	538:567	The extracted crude polysaccharides were purified using ion-exchange and gel-filtration chromatographies and eluted a single symmetrical narrow peak, showing a homogenous fraction (MRP-P1) with a molecular weight of 4.87 × 104 Da.					
32755702	4	0	theme	side	703:706	arg1	rhamnose					748:755	rhamnose	748:755	rhamnose	748:755	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	4	0	theme	side	703:706	arg1	units					720:724	the side chain sugar units	699:724	the side chain sugar units such as arabinose and rhamnose of the hemicelluloses	699:777	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	4	0	theme	side	703:706	arg1	arabinose					734:742	arabinose	734:742	arabinose	734:742	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	1	1	theme	sesame	155:160	arg1	hulls					162:166	sesame hulls	155:166	sesame hulls	155:166	In this investigation, the hemicellulose fractions were isolated from sesame hulls before and after roasting at 160 °C, 180 °C, 200 °C, and 220 °C.					
32755702	5	2	theme	seed	906:909	arg1	hulls					911:915	sesame seed hulls	899:915	sesame seed hulls	899:915	That the main chains of hemicelluloses in the cell wall of sesame seed hulls did not change significantly.					
32755702	5	3	from	wall	891:894	arg1	chains					854:859	That the main chains	840:859	That the main chains of hemicelluloses in the cell wall of sesame seed hulls	840:915	That the main chains of hemicelluloses in the cell wall of sesame seed hulls did not change significantly.					
32755702	2	4	theme	hemicelluloses	266:279	arg1	properties					248:257	The structural properties	233:257	The structural properties of the hemicelluloses	233:279	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	3	5	dep	220 °C	475:480	arg1	to					472:473	to	472:473	to	472:473	Roasting at temperatures from 180 to 220 °C resulted in higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method.					
32755702	3	6	theme	sesame	553:558	arg1	hulls					560:564	sesame hulls	553:564	sesame hulls	553:564	Roasting at temperatures from 180 to 220 °C resulted in higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method.					
32755702	1	7	attach	isolated	141:148	arg1	hulls					162:166	sesame hulls	155:166	sesame hulls	155:166	In this investigation, the hemicellulose fractions were isolated from sesame hulls before and after roasting at 160 °C, 180 °C, 200 °C, and 220 °C.					
32755702	1	7	attach	isolated	141:148	arg2	fractions					126:134	the hemicellulose fractions	108:134	the hemicellulose fractions	108:134	In this investigation, the hemicellulose fractions were isolated from sesame hulls before and after roasting at 160 °C, 180 °C, 200 °C, and 220 °C.					
32755702	5	8	theme	That	840:843	arg1	chains					854:859	That the main chains	840:859	That the main chains of hemicelluloses in the cell wall of sesame seed hulls	840:915	That the main chains of hemicelluloses in the cell wall of sesame seed hulls did not change significantly.					
32755702	6	9	theme	changes	1029:1035	arg1	mechanisms					1000:1009	the mechanisms	996:1009	the mechanisms of the structural changes that occur in sesame hull hemicelluloses during roasting	996:1092	The investigation provides new information about the mechanisms of the structural changes that occur in sesame hull hemicelluloses during roasting.					
32755702	4	10	theme	hemicelluloses	670:683	arg1	analysis					649:656	average molecular weight analysis	624:656	average molecular weight analysis	624:656	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	4	10	theme	hemicelluloses	670:683	arg1	analysis					611:618	sugar analysis	605:618	sugar analysis	605:618	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	0	11	theme	sesame	41:46	arg1	hull					48:51	sesame hull	41:51	sesame hull	41:51	Elucidation of the structural changes of sesame hull hemicelluloses during roasting.					
32755702	4	12	theme	hemicelluloses	764:777	arg1	rhamnose					748:755	rhamnose	748:755	rhamnose	748:755	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	4	12	theme	hemicelluloses	764:777	arg1	arabinose					734:742	arabinose	734:742	arabinose	734:742	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	2	13	theme	weight	394:399	arg1	analysis					401:408	molecular weight analysis	384:408	molecular weight analysis	384:408	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	3	14	from	220 °C	475:480	arg1	temperatures					450:461	temperatures	450:461	temperatures from 180 to 220 °C	450:480	Roasting at temperatures from 180 to 220 °C resulted in higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method.					
32755702	2	15	theme	molecular	384:392	arg1	analysis					401:408	molecular weight analysis	384:408	molecular weight analysis	384:408	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	6	16	theme	new	974:976	arg1	information					978:988	new information	974:988	new information about the mechanisms of the structural changes that occur in sesame hull hemicelluloses during roasting	974:1092	The investigation provides new information about the mechanisms of the structural changes that occur in sesame hull hemicelluloses during roasting.					
32755702	3	17	theme	alkaline	573:580	arg1	method					582:587	the alkaline method	569:587	the alkaline method	569:587	Roasting at temperatures from 180 to 220 °C resulted in higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method.					
32755702	4	18	theme	hull	665:668	arg1	hemicelluloses					670:683	the hull hemicelluloses	661:683	the hull hemicelluloses	661:683	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	0	19	theme	structural	19:28	arg1	changes					30:36	the structural changes	15:36	the structural changes of sesame hull	15:51	Elucidation of the structural changes of sesame hull hemicelluloses during roasting.					
32755702	1	20	dep	isolated	141:148	arg1	before					168:173	before	168:173	before	168:173	In this investigation, the hemicellulose fractions were isolated from sesame hulls before and after roasting at 160 °C, 180 °C, 200 °C, and 220 °C.					
32755702	1	20	dep	isolated	141:148	arg1	roasting					185:192	roasting	185:192	roasting at 160 °C, 180 °C, 200 °C, and 220 °C	185:230	In this investigation, the hemicellulose fractions were isolated from sesame hulls before and after roasting at 160 °C, 180 °C, 200 °C, and 220 °C.					
32755702	4	21	theme	molecular	632:640	arg1	weight					642:647	average molecular weight	624:647	average molecular weight analysis	624:656	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	3	22	attach	isolated	539:546	arg2	hemicelluloses					511:524	hemicelluloses	511:524	hemicelluloses subsequently isolated from sesame hulls by the alkaline method	511:587	Roasting at temperatures from 180 to 220 °C resulted in higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method.					
32755702	3	22	attach	isolated	539:546	arg1	hulls					560:564	sesame hulls	553:564	sesame hulls	553:564	Roasting at temperatures from 180 to 220 °C resulted in higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method.					
32755702	4	23	theme	average	624:630	arg1	weight					642:647	average molecular weight	624:647	average molecular weight analysis	624:656	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	3	24	theme	higher	494:499	arg1	yields					501:506	higher yields	494:506	higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method	494:587	Roasting at temperatures from 180 to 220 °C resulted in higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method.					
32755702	4	25	theme	chain	708:712	arg1	rhamnose					748:755	rhamnose	748:755	rhamnose	748:755	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	4	25	theme	chain	708:712	arg1	units					720:724	the side chain sugar units	699:724	the side chain sugar units such as arabinose and rhamnose of the hemicelluloses	699:777	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	4	25	theme	chain	708:712	arg1	arabinose					734:742	arabinose	734:742	arabinose	734:742	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	4	26	theme	analysis	649:656	arg1	results					594:600	The results	590:600	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses	590:683	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	6	27	theme	hull	1058:1061	arg1	hemicelluloses					1063:1076	sesame hull hemicelluloses	1051:1076	sesame hull hemicelluloses	1051:1076	The investigation provides new information about the mechanisms of the structural changes that occur in sesame hull hemicelluloses during roasting.					
32755702	2	28	theme	thermal	411:417	arg1	analysis					419:426	thermal analysis	411:426	thermal analysis	411:426	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	0	29	theme	changes	30:36	arg1	Elucidation					0:10	Elucidation	0:10	Elucidation of the structural changes of sesame hull	0:51	Elucidation of the structural changes of sesame hull hemicelluloses during roasting.					
32755702	5	30	theme	hemicelluloses	864:877	arg1	chains					854:859	That the main chains	840:859	That the main chains of hemicelluloses in the cell wall of sesame seed hulls	840:915	That the main chains of hemicelluloses in the cell wall of sesame seed hulls did not change significantly.					
32755702	5	31	theme	main	849:852	arg1	chains					854:859	That the main chains	840:859	That the main chains of hemicelluloses in the cell wall of sesame seed hulls	840:915	That the main chains of hemicelluloses in the cell wall of sesame seed hulls did not change significantly.					
32755702	4	32	theme	weight	642:647	arg1	analysis					649:656	average molecular weight analysis	624:656	average molecular weight analysis	624:656	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	0	33	theme	hull	48:51	arg1	changes					30:36	the structural changes	15:36	the structural changes of sesame hull	15:51	Elucidation of the structural changes of sesame hull hemicelluloses during roasting.					
32755702	3	34	from	temperatures	450:461	arg1	Roasting					438:445	Roasting	438:445	Roasting at temperatures from 180 to 220 °C	438:480	Roasting at temperatures from 180 to 220 °C resulted in higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method.					
32755702	4	35	theme	sugar	714:718	arg1	rhamnose					748:755	rhamnose	748:755	rhamnose	748:755	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	4	35	theme	sugar	714:718	arg1	units					720:724	the side chain sugar units	699:724	the side chain sugar units such as arabinose and rhamnose of the hemicelluloses	699:777	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	4	35	theme	sugar	714:718	arg1	arabinose					734:742	arabinose	734:742	arabinose	734:742	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	2	36	theme	compositions	361:372	arg1	analysis					374:381	sugar compositions analysis	355:381	sugar compositions analysis	355:381	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	1	37	theme	hemicellulose	112:124	arg1	fractions					126:134	the hemicellulose fractions	108:134	the hemicellulose fractions	108:134	In this investigation, the hemicellulose fractions were isolated from sesame hulls before and after roasting at 160 °C, 180 °C, 200 °C, and 220 °C.					
32755702	2	38	theme	structural	237:246	arg1	properties					248:257	The structural properties	233:257	The structural properties of the hemicelluloses	233:279	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	5	39	theme	hulls	911:915	arg1	wall					891:894	the cell wall	882:894	the cell wall of sesame seed hulls	882:915	That the main chains of hemicelluloses in the cell wall of sesame seed hulls did not change significantly.					
32755702	4	40	theme	sugar	605:609	arg1	analysis					611:618	sugar analysis	605:618	sugar analysis	605:618	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
32755702	5	41	theme	sesame	899:904	arg1	hulls					911:915	sesame seed hulls	899:915	sesame seed hulls	899:915	That the main chains of hemicelluloses in the cell wall of sesame seed hulls did not change significantly.					
32755702	5	42	from	hemicelluloses	864:877	arg1	wall					891:894	the cell wall	882:894	the cell wall of sesame seed hulls	882:915	That the main chains of hemicelluloses in the cell wall of sesame seed hulls did not change significantly.					
32755702	2	43	theme	analytical	316:325	arg1	analysis					401:408	molecular weight analysis	384:408	molecular weight analysis	384:408	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	2	43	theme	analytical	316:325	arg1	NMR					433:435	NMR	433:435	NMR	433:435	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	2	43	theme	analytical	316:325	arg1	analysis					374:381	sugar compositions analysis	355:381	sugar compositions analysis	355:381	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	2	43	theme	analytical	316:325	arg1	analysis					419:426	thermal analysis	411:426	thermal analysis	411:426	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	2	43	theme	analytical	316:325	arg1	techniques					327:336	analytical techniques	316:336	analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR	316:435	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	2	43	theme	analytical	316:325	arg1	FT-IR					348:352	FT-IR	348:352	FT-IR	348:352	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	6	44	theme	structural	1018:1027	arg1	changes					1029:1035	the structural changes	1014:1035	the structural changes that occur in sesame hull hemicelluloses during roasting	1014:1092	The investigation provides new information about the mechanisms of the structural changes that occur in sesame hull hemicelluloses during roasting.					
32755702	5	45	theme	cell	886:889	arg1	wall					891:894	the cell wall	882:894	the cell wall of sesame seed hulls	882:915	That the main chains of hemicelluloses in the cell wall of sesame seed hulls did not change significantly.					
32755702	6	46	theme	sesame	1051:1056	arg1	hemicelluloses					1063:1076	sesame hull hemicelluloses	1051:1076	sesame hull hemicelluloses	1051:1076	The investigation provides new information about the mechanisms of the structural changes that occur in sesame hull hemicelluloses during roasting.					
32755702	2	47	theme	sugar	355:359	arg1	analysis					374:381	sugar compositions analysis	355:381	sugar compositions analysis	355:381	The structural properties of the hemicelluloses were comparatively investigated by analytical techniques including FT-IR, sugar compositions analysis, molecular weight analysis, thermal analysis, and NMR.					
32755702	5	48	from	chains	854:859	arg1	wall					891:894	the cell wall	882:894	the cell wall of sesame seed hulls	882:915	That the main chains of hemicelluloses in the cell wall of sesame seed hulls did not change significantly.					
32755702	3	49	theme	hemicelluloses	511:524	arg1	yields					501:506	higher yields	494:506	higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method	494:587	Roasting at temperatures from 180 to 220 °C resulted in higher yields of hemicelluloses subsequently isolated from sesame hulls by the alkaline method.					
32755702	4	50	theme	analysis	611:618	arg1	results					594:600	The results	590:600	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses	590:683	The results of sugar analysis and average molecular weight analysis of the hull hemicelluloses revealed that the side chain sugar units such as arabinose and rhamnose of the hemicelluloses are more easily degraded after roasting during the roasting.					
31975691	7	0	theme	unclassified	1325:1336	arg1	Alteromonadales					1338:1352	unclassified Alteromonadales	1325:1352	unclassified Alteromonadales	1325:1352	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
31975691	4	1	theme	bacterial	697:705	arg1	communities					707:717	bacterial communities	697:717	bacterial communities	697:717	Our results show that bacterial communities responded to the increased availability of macroalgal polysaccharides and community shift was congruent with a significant decline in nutrient concentrations.					
31975691	1	2	theme	Arctic	153:158	arg1	habitats					160:167	coastal Arctic habitats	145:167	coastal Arctic habitats	145:167	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	6	3	theme	Each	1002:1005	arg1	addition					1022:1029	Each polysaccharide addition	1002:1029	Each polysaccharide addition	1002:1029	Each polysaccharide addition incited the growth of certain distinct bacteria taxa.					
31975691	7	4	theme	AL	1231:1232	arg1	addition					1234:1241	AL addition	1231:1241	AL addition	1231:1241	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
31975691	2	5	from	detritus	358:365	arg1	region					374:379	the region	370:379	the region	370:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	2	6	dep	cover	297:301	arg1	contribute					303:312	contribute	303:312	cover contribute a significant amount of polysaccharide-rich detritus in the region	297:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	0	7	theme	macroalgal	80:89	arg1	amendments					106:115	macroalgal polysaccharide amendments	80:115	macroalgal polysaccharide amendments	80:115	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	1	8	from	abundant	133:140	arg1	habitats					160:167	coastal Arctic habitats	145:167	coastal Arctic habitats	145:167	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	1	9	from	habitats	160:167	arg1	abundant					133:140	abundant	133:140	abundant	133:140	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	2	10	from	amount	328:333	arg1	region					374:379	the region	370:379	the region	370:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	6	11	theme	bacteria	1070:1077	arg1	taxa					1079:1082	certain distinct bacteria taxa	1053:1082	certain distinct bacteria taxa	1053:1082	Each polysaccharide addition incited the growth of certain distinct bacteria taxa.					
31975691	4	12	from	decline	842:848	arg1	concentrations					862:875	nutrient concentrations	853:875	nutrient concentrations	853:875	Our results show that bacterial communities responded to the increased availability of macroalgal polysaccharides and community shift was congruent with a significant decline in nutrient concentrations.					
31975691	6	13	theme	taxa	1079:1082	arg1	growth					1043:1048	the growth	1039:1048	the growth of certain distinct bacteria taxa	1039:1082	Each polysaccharide addition incited the growth of certain distinct bacteria taxa.					
31975691	3	14	from	Svalbard	566:573	arg1	communities					535:545	bacterial communities	525:545	bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean)	525:588	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	3	14	from	Svalbard	566:573	arg1	response					513:520	the response	509:520	the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments	509:633	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	4	15	theme	community	793:801	arg1	shift					803:807	community shift	793:807	community shift	793:807	Our results show that bacterial communities responded to the increased availability of macroalgal polysaccharides and community shift was congruent with a significant decline in nutrient concentrations.					
31975691	3	16	theme	bacterial	525:533	arg1	communities					535:545	bacterial communities	525:545	bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean)	525:588	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	3	17	from	degradation	401:411	arg1	processes					488:496	biogeochemical processes	473:496	biogeochemical processes	473:496	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	5	18	theme	bacterial	893:901	arg1	taxa					903:906	Initially-rare bacterial taxa	878:906	Initially-rare bacterial taxa affiliated with Gammaproteobacteria and Bacteroidia	878:958	Initially-rare bacterial taxa affiliated with Gammaproteobacteria and Bacteroidia responded to the polysaccharide addition.					
31975691	3	19	theme	Arctic	576:581	arg1	Svalbard					566:573	Svalbard	566:573	Svalbard	566:573	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	3	19	theme	Arctic	576:581	arg1	Ocean					583:587	Arctic Ocean	576:587	Arctic Ocean	576:587	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	3	20	theme	biogeochemical	473:486	arg1	processes					488:496	biogeochemical processes	473:496	biogeochemical processes	473:496	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	0	21	theme	polysaccharide	91:104	arg1	amendments					106:115	macroalgal polysaccharide amendments	80:115	macroalgal polysaccharide amendments	80:115	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	0	22	from	Kongsfjorden	39:50	arg1	Response					0:7	Response	0:7	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.	0:116	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	0	22	from	Kongsfjorden	39:50	arg1	communities					22:32	bacterial communities	12:32	bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean)	12:75	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	7	23	theme	unclassified	1185:1196	arg1	Gammaproteobacteria					1198:1216	unclassified Gammaproteobacteria	1185:1216	unclassified Gammaproteobacteria	1185:1216	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
31975691	7	24	dep	responded	1218:1226	arg1	whereas					1244:1250	whereas	1244:1250	whereas	1244:1250	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
31975691	8	25	from	carbon	1559:1564	arg1	Svalbard					1598:1605	coastal Svalbard	1590:1605	coastal Svalbard	1590:1605	These results suggest that polysaccharides shift bacterial community composition towards copiotrophic bacterial taxa, with implications for carbon and nutrient cycling in coastal Svalbard.					
31975691	5	26	theme	polysaccharide	977:990	arg1	addition					992:999	the polysaccharide addition	973:999	the polysaccharide addition	973:999	Initially-rare bacterial taxa affiliated with Gammaproteobacteria and Bacteroidia responded to the polysaccharide addition.					
31975691	2	27	from	region	374:379	arg1	amount					328:333	a significant amount	314:333	a significant amount of polysaccharide-rich detritus in the region	314:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	2	27	from	region	374:379	arg1	detritus					358:365	polysaccharide-rich detritus	338:365	polysaccharide-rich detritus in the region	338:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	4	28	theme	macroalgal	762:771	arg1	polysaccharides					773:787	macroalgal polysaccharides	762:787	macroalgal polysaccharides	762:787	Our results show that bacterial communities responded to the increased availability of macroalgal polysaccharides and community shift was congruent with a significant decline in nutrient concentrations.					
31975691	7	29	theme	AG	1406:1407	arg1	addition					1409:1416	the AG addition	1402:1416	the AG addition	1402:1416	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
31975691	2	30	theme	macroalgal	226:235	arg1	productivity					237:248	macroalgal productivity	226:248	macroalgal productivity due to warmer temperatures	226:275	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	3	31	theme	macroalgal	416:425	arg1	polysaccharides					427:441	macroalgal polysaccharides	416:441	macroalgal polysaccharides	416:441	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	4	32	with	congruent	813:821	arg1	decline					842:848	a significant decline	828:848	a significant decline in nutrient concentrations	828:875	Our results show that bacterial communities responded to the increased availability of macroalgal polysaccharides and community shift was congruent with a significant decline in nutrient concentrations.					
31975691	4	33	theme	increased	736:744	arg1	availability					746:757	the increased availability	732:757	the increased availability of macroalgal polysaccharides	732:787	Our results show that bacterial communities responded to the increased availability of macroalgal polysaccharides and community shift was congruent with a significant decline in nutrient concentrations.					
31975691	1	34	theme	large	183:187	arg1	polysaccharides					199:213	polysaccharides	199:213	polysaccharides	199:213	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	1	34	theme	large	183:187	arg1	amount					189:194	a large amount	181:194	a large amount of polysaccharides	181:213	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	0	35	theme	communities	22:32	arg1	Response					0:7	Response	0:7	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.	0:116	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	6	36	theme	distinct	1061:1068	arg1	taxa					1079:1082	certain distinct bacteria taxa	1053:1082	certain distinct bacteria taxa	1053:1082	Each polysaccharide addition incited the growth of certain distinct bacteria taxa.					
31975691	2	37	theme	significant	316:326	arg1	amount					328:333	a significant amount	314:333	a significant amount of polysaccharide-rich detritus in the region	314:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	2	37	theme	significant	316:326	arg1	detritus					358:365	polysaccharide-rich detritus	338:365	polysaccharide-rich detritus in the region	338:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	5	38	theme	Initially-rare	878:891	arg1	taxa					903:906	Initially-rare bacterial taxa	878:906	Initially-rare bacterial taxa affiliated with Gammaproteobacteria and Bacteroidia	878:958	Initially-rare bacterial taxa affiliated with Gammaproteobacteria and Bacteroidia responded to the polysaccharide addition.					
31975691	0	39	theme	bacterial	12:20	arg1	communities					22:32	bacterial communities	12:32	bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean)	12:75	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	6	40	theme	certain	1053:1059	arg1	taxa					1079:1082	certain distinct bacteria taxa	1053:1082	certain distinct bacteria taxa	1053:1082	Each polysaccharide addition incited the growth of certain distinct bacteria taxa.					
31975691	8	41	theme	bacterial	1521:1529	arg1	taxa					1531:1534	copiotrophic bacterial taxa	1508:1534	copiotrophic bacterial taxa	1508:1534	These results suggest that polysaccharides shift bacterial community composition towards copiotrophic bacterial taxa, with implications for carbon and nutrient cycling in coastal Svalbard.					
31975691	3	42	theme	microcosm	653:661	arg1	experiment					663:672	an ex-situ microcosm experiment	642:672	an ex-situ microcosm experiment	642:672	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	4	43	theme	polysaccharides	773:787	arg1	availability					746:757	the increased availability	732:757	the increased availability of macroalgal polysaccharides	732:787	Our results show that bacterial communities responded to the increased availability of macroalgal polysaccharides and community shift was congruent with a significant decline in nutrient concentrations.					
31975691	2	44	dep	Increased	216:224	arg1	cover					297:301	cover	297:301	cover contribute a significant amount of polysaccharide-rich detritus in the region	297:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	8	45	theme	copiotrophic	1508:1519	arg1	taxa					1531:1534	copiotrophic bacterial taxa	1508:1534	copiotrophic bacterial taxa	1508:1534	These results suggest that polysaccharides shift bacterial community composition towards copiotrophic bacterial taxa, with implications for carbon and nutrient cycling in coastal Svalbard.					
31975691	8	46	theme	bacterial	1468:1476	arg1	composition					1488:1498	bacterial community composition	1468:1498	bacterial community composition towards copiotrophic bacterial taxa	1468:1534	These results suggest that polysaccharides shift bacterial community composition towards copiotrophic bacterial taxa, with implications for carbon and nutrient cycling in coastal Svalbard.					
31975691	4	47	theme	nutrient	853:860	arg1	concentrations					862:875	nutrient concentrations	853:875	nutrient concentrations	853:875	Our results show that bacterial communities responded to the increased availability of macroalgal polysaccharides and community shift was congruent with a significant decline in nutrient concentrations.					
31975691	8	48	from	cycling	1579:1585	arg1	Svalbard					1598:1605	coastal Svalbard	1590:1605	coastal Svalbard	1590:1605	These results suggest that polysaccharides shift bacterial community composition towards copiotrophic bacterial taxa, with implications for carbon and nutrient cycling in coastal Svalbard.					
31975691	1	49	theme	polysaccharides	199:213	arg1	polysaccharides					199:213	polysaccharides	199:213	polysaccharides	199:213	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	1	49	theme	polysaccharides	199:213	arg1	amount					189:194	a large amount	181:194	a large amount of polysaccharides	181:213	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	3	50	theme	potential	453:461	arg1	impact					463:468	their potential impact	447:468	their potential impact on biogeochemical processes	447:496	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	1	51	contain	contain	173:179	arg2	polysaccharides					199:213	polysaccharides	199:213	polysaccharides	199:213	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	1	51	contain	contain	173:179	arg1	Macroalgae					118:127	Macroalgae	118:127	Macroalgae	118:127	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	1	51	contain	contain	173:179	arg2	amount					189:194	a large amount	181:194	a large amount of polysaccharides	181:213	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	3	52	theme	bacterial	391:399	arg1	degradation					401:411	bacterial degradation	391:411	bacterial degradation of macroalgal polysaccharides	391:441	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	7	53	theme	control	1112:1118	arg1	CM					1132:1133	CM	1132:1133	CM	1132:1133	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
31975691	7	53	theme	control	1112:1118	arg1	microcosms					1120:1129	the un-amended control microcosms	1097:1129	the un-amended control microcosms (CM)	1097:1134	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
31975691	7	54	theme	unclassified	1291:1302	arg1	Gammaproteobacteria					1304:1322	unclassified Gammaproteobacteria	1291:1322	unclassified Gammaproteobacteria	1291:1322	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
31975691	3	55	theme	polysaccharides	427:441	arg1	impact					463:468	their potential impact	447:468	their potential impact on biogeochemical processes	447:496	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	3	55	theme	polysaccharides	427:441	arg1	degradation					401:411	bacterial degradation	391:411	bacterial degradation of macroalgal polysaccharides	391:441	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	3	56	theme	ex-situ	645:651	arg1	experiment					663:672	an ex-situ microcosm experiment	642:672	an ex-situ microcosm experiment	642:672	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	0	57	dep	Kongsfjorden	39:50	arg1	Ocean					70:74	Arctic Ocean	63:74	Arctic Ocean	63:74	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	0	57	dep	Kongsfjorden	39:50	arg1	Svalbard					53:60	Svalbard	53:60	Svalbard	53:60	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	3	58	theme	communities	535:545	arg1	response					513:520	the response	509:520	the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments	509:633	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	0	59	from	Response	0:7	arg1	Kongsfjorden					39:50	Kongsfjorden	39:50	Kongsfjorden	39:50	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	8	60	theme	nutrient	1570:1577	arg1	cycling					1579:1585	nutrient cycling	1570:1585	nutrient cycling in coastal Svalbard	1570:1605	These results suggest that polysaccharides shift bacterial community composition towards copiotrophic bacterial taxa, with implications for carbon and nutrient cycling in coastal Svalbard.					
31975691	3	61	theme	agarose	611:617	arg1	amendments					624:633	alginate (AL) and agarose (AG) amendments	593:633	amendments	624:633	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	3	61	theme	agarose	611:617	arg1	AG					620:621	AG	620:621	AG	620:621	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	8	62	theme	coastal	1590:1596	arg1	Svalbard					1598:1605	coastal Svalbard	1590:1605	coastal Svalbard	1590:1605	These results suggest that polysaccharides shift bacterial community composition towards copiotrophic bacterial taxa, with implications for carbon and nutrient cycling in coastal Svalbard.					
31975691	3	63	from	response	513:520	arg1	Svalbard					566:573	Svalbard	566:573	Svalbard	566:573	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	3	63	from	response	513:520	arg1	Ocean					583:587	Arctic Ocean	576:587	Arctic Ocean	576:587	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	2	64	theme	detritus	358:365	arg1	amount					328:333	a significant amount	314:333	a significant amount of polysaccharide-rich detritus in the region	314:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	2	64	theme	detritus	358:365	arg1	detritus					358:365	polysaccharide-rich detritus	338:365	polysaccharide-rich detritus in the region	338:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	7	65	theme	un-amended	1101:1110	arg1	CM					1132:1133	CM	1132:1133	CM	1132:1133	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
31975691	7	65	theme	un-amended	1101:1110	arg1	microcosms					1120:1129	the un-amended control microcosms	1097:1129	the un-amended control microcosms (CM)	1097:1134	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
31975691	3	66	dep	studied	501:507	arg1	study					385:389	study	385:389	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes	382:496	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	3	67	from	impact	463:468	arg1	processes					488:496	biogeochemical processes	473:496	biogeochemical processes	473:496	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	2	68	theme	polysaccharide-rich	338:356	arg1	detritus					358:365	polysaccharide-rich detritus	338:365	polysaccharide-rich detritus in the region	338:379	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	8	69	theme	community	1478:1486	arg1	composition					1488:1498	bacterial community composition	1468:1498	bacterial community composition towards copiotrophic bacterial taxa	1468:1534	These results suggest that polysaccharides shift bacterial community composition towards copiotrophic bacterial taxa, with implications for carbon and nutrient cycling in coastal Svalbard.					
31975691	0	70	theme	Arctic	63:68	arg1	Ocean					70:74	Arctic Ocean	63:74	Arctic Ocean	63:74	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	0	70	theme	Arctic	63:68	arg1	Svalbard					53:60	Svalbard	53:60	Svalbard	53:60	Response of bacterial communities from Kongsfjorden (Svalbard, Arctic Ocean) to macroalgal polysaccharide amendments.					
31975691	2	71	theme	warmer	257:262	arg1	temperatures					264:275	warmer temperatures	257:275	warmer temperatures	257:275	Increased macroalgal productivity due to warmer temperatures and reduced sea-ice cover contribute a significant amount of polysaccharide-rich detritus in the region.					
31975691	3	72	dep	alginate	593:600	arg1	AL					603:604	AL	603:604	AL	603:604	To study bacterial degradation of macroalgal polysaccharides and their potential impact on biogeochemical processes we studied the response of bacterial communities from Kongsfjorden, Svalbard (Arctic Ocean) to alginate (AL) and agarose (AG) amendments, using an ex-situ microcosm experiment.					
31975691	6	73	theme	polysaccharide	1007:1020	arg1	addition					1022:1029	Each polysaccharide addition	1002:1029	Each polysaccharide addition	1002:1029	Each polysaccharide addition incited the growth of certain distinct bacteria taxa.					
31975691	4	74	theme	significant	830:840	arg1	decline					842:848	a significant decline	828:848	a significant decline in nutrient concentrations	828:875	Our results show that bacterial communities responded to the increased availability of macroalgal polysaccharides and community shift was congruent with a significant decline in nutrient concentrations.					
31975691	1	75	theme	coastal	145:151	arg1	habitats					160:167	coastal Arctic habitats	145:167	coastal Arctic habitats	145:167	Macroalgae are abundant in coastal Arctic habitats and contain a large amount of polysaccharides.					
31975691	7	76	theme	unclassified	1359:1370	arg1	Alteromonadaceae					1372:1387	unclassified Alteromonadaceae	1359:1387	unclassified Alteromonadaceae	1359:1387	Compared to the un-amended control microcosms (CM), Polaribacter, Colwellia, Pseudoalteromonas, and unclassified Gammaproteobacteria responded to AL addition, whereas Paraglaciecola, Lentimonas, Colwellia, unclassified Gammaproteobacteria, unclassified Alteromonadales, and unclassified Alteromonadaceae responded to the AG addition.					
32244921	8	0	dep	technique	1695:1703	arg1	orders					1734:1739	approximately three or four orders	1706:1739	approximately three or four orders of magnitude faster than CRM	1706:1768	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	8	1	used	used	1776:1779	arg2	technique					1695:1703	an ultrafast Raman imaging technique	1668:1703	an ultrafast Raman imaging technique	1668:1703	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	8	1	used	used	1776:1779	arg2	SRS					1654:1656	SRS	1654:1656	SRS	1654:1656	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	6	2	theme	label-free	1239:1248	arg1	way					1250:1252	a label-free way	1237:1252	a label-free way	1237:1252	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	11	3	theme	CRM	2414:2416	arg1	method					2449:2454	a promising method	2437:2454	a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach	2437:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	11	3	theme	CRM	2414:2416	arg1	use					2407:2409	the hybrid use	2396:2409	the hybrid use of CRM, FTIRM, and SRS	2396:2432	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	7	4	theme	cell	1443:1446	arg1	wall					1448:1451	the cell wall	1439:1451	the cell wall of peach flesh	1439:1466	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	11	5	theme	simultaneous	2460:2471	arg1	visualization					2473:2485	simultaneous visualization	2460:2485	simultaneous visualization of changes in cell wall polysaccharides of peach	2460:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	10	6	with	line	2176:2179	arg1	trends					2206:2211	the whole softening trends	2186:2211	the whole softening trends	2186:2211	In general, cell wall polysaccharides decreased during softening and tended to be concentrated in the cell corner for most parenchymal cells at the late stage, but there were also some cells not in line with the whole softening trends.					
32244921	9	7	theme	Different	1835:1843	arg1	degrees					1857:1863	Different degradation degrees	1835:1863	Different degradation degrees of parenchymal cells during fruit softening	1835:1907	Different degradation degrees of parenchymal cells during fruit softening were found based on the gray-scale statistical analysis of SRS data.					
32244921	6	8	theme	peach	1222:1226	arg1	fruit					1228:1232	peach fruit	1222:1232	peach fruit	1222:1232	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	10	9	theme	softening	2196:2204	arg1	trends					2206:2211	the whole softening trends	2186:2211	the whole softening trends	2186:2211	In general, cell wall polysaccharides decreased during softening and tended to be concentrated in the cell corner for most parenchymal cells at the late stage, but there were also some cells not in line with the whole softening trends.					
32244921	11	10	theme	wall	2506:2509	arg1	polysaccharides					2511:2525	cell wall polysaccharides	2501:2525	cell wall polysaccharides of peach	2501:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	7	11	theme	spatial	1380:1386	arg1	changes					1388:1394	the spatial changes	1376:1394	the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh	1376:1466	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	6	12	theme	polysaccharides	1203:1217	arg1	distribution					1177:1188	the spatial distribution	1165:1188	the spatial distribution of cell wall polysaccharides of peach fruit	1165:1232	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	3	13	theme	spatial	567:573	arg1	distribution					575:586	the spatial distribution	563:586	the spatial distribution of cell wall polysaccharides	563:615	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	5	14	theme	°C.	848:850	arg1	measurement					861:871	20 °C. Firmness measurement	845:871	20 °C. Firmness measurement	845:871	"Zhonghuashoutao") at commercial maturity were stored at 0 °C and 20 °C. Firmness measurement and chemical analysis were performed at each storage time.					
32244921	9	15	theme	parenchymal	1868:1878	arg1	cells					1880:1884	parenchymal cells	1868:1884	parenchymal cells	1868:1884	Different degradation degrees of parenchymal cells during fruit softening were found based on the gray-scale statistical analysis of SRS data.					
32244921	4	16	theme	Nonmelting	731:740	arg1	peaches					742:748	Nonmelting peaches	731:748	Nonmelting peaches (Prunus persica L. Batsch cv.	731:778	Nonmelting peaches (Prunus persica L. Batsch cv.					
32244921	11	17	theme	polysaccharides	2307:2321	arg1	content					2266:2272	content	2266:2272	content	2266:2272	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	11	17	theme	polysaccharides	2307:2321	arg1	changes					2286:2292	spatial changes	2278:2292	spatial changes	2278:2292	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	8	18	theme	ultrafast	1671:1679	arg1	technique					1695:1703	an ultrafast Raman imaging technique	1668:1703	an ultrafast Raman imaging technique	1668:1703	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	8	18	theme	ultrafast	1671:1679	arg1	SRS					1654:1656	SRS	1654:1656	SRS	1654:1656	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	1	19	theme	postharvest	215:225	arg1	storage					227:233	the postharvest storage	211:233	the postharvest storage	211:233	Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall, affects the texture and quality of fruit.					
32244921	11	20	theme	FTIRM	2419:2423	arg1	method					2449:2454	a promising method	2437:2454	a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach	2437:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	11	20	theme	FTIRM	2419:2423	arg1	use					2407:2409	the hybrid use	2396:2409	the hybrid use of CRM, FTIRM, and SRS	2396:2432	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	6	21	theme	wall	1198:1201	arg1	polysaccharides					1203:1217	cell wall polysaccharides	1193:1217	cell wall polysaccharides	1193:1217	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	3	22	theme	wall	596:599	arg1	polysaccharides					601:615	cell wall polysaccharides	591:615	cell wall polysaccharides	591:615	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	8	23	theme	imaging	1687:1693	arg1	technique					1695:1703	an ultrafast Raman imaging technique	1668:1703	an ultrafast Raman imaging technique	1668:1703	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	8	23	theme	imaging	1687:1693	arg1	SRS					1654:1656	SRS	1654:1656	SRS	1654:1656	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	10	24	theme	whole	2190:2194	arg1	trends					2206:2211	the whole softening trends	2186:2211	the whole softening trends	2186:2211	In general, cell wall polysaccharides decreased during softening and tended to be concentrated in the cell corner for most parenchymal cells at the late stage, but there were also some cells not in line with the whole softening trends.					
32244921	0	25	from	Changes	75:81	arg1	Polysaccharides					96:110	Cell Wall Polysaccharides	86:110	Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage	86:182	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	7	26	theme	complementary	1328:1340	arg1	information					1351:1361	complementary spectral information	1328:1361	complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level	1328:1508	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	10	27	theme	parenchymal	2101:2111	arg1	cells					2113:2117	most parenchymal cells	2096:2117	most parenchymal cells	2096:2117	In general, cell wall polysaccharides decreased during softening and tended to be concentrated in the cell corner for most parenchymal cells at the late stage, but there were also some cells not in line with the whole softening trends.					
32244921	6	28	theme	postharvest	1265:1275	arg1	storage					1277:1283	the postharvest storage	1261:1283	the postharvest storage	1261:1283	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	7	29	theme	FTIRM	1313:1317	arg1	combination					1290:1300	The combination	1286:1300	The combination of CRM and FTIRM	1286:1317	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	6	30	theme	spatial	1169:1175	arg1	distribution					1177:1188	the spatial distribution	1165:1188	the spatial distribution of cell wall polysaccharides of peach fruit	1165:1232	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	8	31	theme	wall	1806:1809	arg1	measurement					1822:1832	high-throughput cell wall phenotypes measurement	1785:1832	high-throughput cell wall phenotypes measurement	1785:1832	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	3	32	theme	flesh	626:630	arg1	cells					632:636	peach flesh cells	620:636	peach flesh cells	620:636	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	6	33	theme	Raman	997:1001	arg1	techniques					969:978	three molecular imaging techniques	945:978	three molecular imaging techniques	945:978	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	33	theme	Raman	997:1001	arg1	CRM					1022:1024	CRM	1022:1024	CRM	1022:1024	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	33	theme	Raman	997:1001	arg1	microspectroscopy					1003:1019	confocal Raman microspectroscopy	988:1019	confocal Raman microspectroscopy (CRM)	988:1025	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	0	34	from	Polysaccharides	96:110	arg1	Visualization					58:70	Simultaneous Visualization	45:70	Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage	45:182	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	7	35	theme	CRM	1305:1307	arg1	combination					1290:1300	The combination	1286:1300	The combination of CRM and FTIRM	1286:1317	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	10	36	theme	late	2126:2129	arg1	stage					2131:2135	the late stage	2122:2135	the late stage	2122:2135	In general, cell wall polysaccharides decreased during softening and tended to be concentrated in the cell corner for most parenchymal cells at the late stage, but there were also some cells not in line with the whole softening trends.					
32244921	11	37	dep	content	2266:2272	arg1	the					2262:2264	the	2262:2264	the	2262:2264	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	0	38	theme	Changes	75:81	arg1	Visualization					58:70	Simultaneous Visualization	45:70	Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage	45:182	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	7	39	theme	cell	1530:1533	arg1	polysaccharides					1540:1554	the cell wall polysaccharides	1526:1554	the cell wall polysaccharides	1526:1554	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	9	40	theme	gray-scale	1933:1942	arg1	analysis					1956:1963	the gray-scale statistical analysis	1929:1963	the gray-scale statistical analysis of SRS data	1929:1975	Different degradation degrees of parenchymal cells during fruit softening were found based on the gray-scale statistical analysis of SRS data.					
32244921	6	41	from	changes	1154:1160	arg1	distribution					1177:1188	the spatial distribution	1165:1188	the spatial distribution of cell wall polysaccharides of peach fruit	1165:1232	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	41	from	changes	1154:1160	arg1	way					1250:1252	a label-free way	1237:1252	a label-free way	1237:1252	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	0	42	theme	Cell	86:89	arg1	Polysaccharides					96:110	Cell Wall Polysaccharides	86:110	Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage	86:182	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	5	43	theme	chemical	877:884	arg1	analysis					886:893	chemical analysis	877:893	chemical analysis	877:893	"Zhonghuashoutao") at commercial maturity were stored at 0 °C and 20 °C. Firmness measurement and chemical analysis were performed at each storage time.					
32244921	6	44	dep	techniques	969:978	arg1	SRS					1126:1128	SRS	1126:1128	SRS	1126:1128	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	44	dep	techniques	969:978	arg1	microscopy					1114:1123	microscopy	1114:1123	microscopy (SRS)	1114:1129	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	11	45	theme	spatial	2278:2284	arg1	changes					2286:2292	spatial changes	2278:2292	spatial changes	2278:2292	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	8	46	theme	faster	1754:1759	arg1	orders					1734:1739	approximately three or four orders	1706:1739	approximately three or four orders of magnitude faster than CRM	1706:1768	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	6	47	theme	molecular	951:959	arg1	techniques					969:978	three molecular imaging techniques	945:978	three molecular imaging techniques	945:978	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	47	theme	molecular	951:959	arg1	microspectroscopy					1003:1019	confocal Raman microspectroscopy	988:1019	confocal Raman microspectroscopy (CRM)	988:1025	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	0	48	from	Visualization	58:70	arg1	Polysaccharides					96:110	Cell Wall Polysaccharides	86:110	Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage	86:182	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	9	49	theme	SRS	1968:1970	arg1	data					1972:1975	SRS data	1968:1975	SRS data	1968:1975	Different degradation degrees of parenchymal cells during fruit softening were found based on the gray-scale statistical analysis of SRS data.					
32244921	0	50	theme	Peach	115:119	arg1	Levels					150:155	Peach at Single- and Multiple-Cell Levels	115:155	Peach at Single- and Multiple-Cell Levels during Postharvest Storage	115:182	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	1	51	theme	fruit	379:383	arg1	texture					356:362	texture	356:362	texture	356:362	Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall, affects the texture and quality of fruit.					
32244921	1	51	theme	fruit	379:383	arg1	quality					368:374	quality	368:374	quality	368:374	Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall, affects the texture and quality of fruit.					
32244921	11	52	theme	wall	2302:2305	arg1	polysaccharides					2307:2321	cell wall polysaccharides	2297:2321	cell wall polysaccharides	2297:2321	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	6	53	dep	transform	1036:1044	arg1	infrared					1046:1053	infrared	1046:1053	transform infrared microspectroscopy (FTIRM)	1036:1079	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	0	54	theme	Hybrid	0:5	arg1	Microscopies					28:39	Hybrid Label-Free Molecular Microscopies	0:39	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.	0:183	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	1	55	theme	spatial	291:297	arg1	changes					299:305	both compositional and spatial changes	268:305	both compositional and spatial changes of polysaccharides within cell wall	268:341	Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall, affects the texture and quality of fruit.					
32244921	11	56	from	polysaccharides	2511:2525	arg1	visualization					2473:2485	simultaneous visualization	2460:2485	simultaneous visualization of changes in cell wall polysaccharides of peach	2460:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	5	57	theme	storage	918:924	arg1	time					926:929	each storage time	913:929	each storage time	913:929	"Zhonghuashoutao") at commercial maturity were stored at 0 °C and 20 °C. Firmness measurement and chemical analysis were performed at each storage time.					
32244921	7	58	theme	cells	1616:1620	arg1	corner					1594:1599	the cell corner	1585:1599	the cell corner of parenchymal cells	1585:1620	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	0	59	theme	Molecular	18:26	arg1	Microscopies					28:39	Hybrid Label-Free Molecular Microscopies	0:39	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.	0:183	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	11	60	from	visualization	2473:2485	arg1	polysaccharides					2511:2525	cell wall polysaccharides	2501:2525	cell wall polysaccharides of peach	2501:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	7	61	theme	single-cell	1492:1502	arg1	level					1504:1508	the single-cell level	1488:1508	the single-cell level	1488:1508	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	11	62	theme	cell	2501:2504	arg1	polysaccharides					2511:2525	cell wall polysaccharides	2501:2525	cell wall polysaccharides of peach	2501:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	3	63	theme	single-	646:652	arg1	levels					672:677	both single- and multiple-cell levels	641:677	both single- and multiple-cell levels in a label-free way	641:697	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	2	64	theme	softening	416:424	arg1	mechanism					426:434	the fruit softening mechanism	406:434	the fruit softening mechanism	406:434	Current research on the fruit softening mechanism lacks an understanding of the overall softening at the cell level.					
32244921	11	65	from	changes	2490:2496	arg1	polysaccharides					2511:2525	cell wall polysaccharides	2501:2525	cell wall polysaccharides of peach	2501:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	2	66	from	research	394:401	arg1	mechanism					426:434	the fruit softening mechanism	406:434	the fruit softening mechanism	406:434	Current research on the fruit softening mechanism lacks an understanding of the overall softening at the cell level.					
32244921	2	67	theme	softening	474:482	arg1	understanding					445:457	an understanding	442:457	an understanding of the overall softening	442:482	Current research on the fruit softening mechanism lacks an understanding of the overall softening at the cell level.					
32244921	8	68	theme	cell	1801:1804	arg1	measurement					1822:1832	high-throughput cell wall phenotypes measurement	1785:1832	high-throughput cell wall phenotypes measurement	1785:1832	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	6	69	theme	Raman	1097:1101	arg1	scattering					1103:1112	stimulated Raman scattering	1086:1112	stimulated Raman scattering	1086:1112	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	11	70	theme	peach	2530:2534	arg1	polysaccharides					2511:2525	cell wall polysaccharides	2501:2525	cell wall polysaccharides of peach	2501:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	7	71	theme	peach	1456:1460	arg1	flesh					1462:1466	peach flesh	1456:1466	peach flesh	1456:1466	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	10	72	theme	cell	1990:1993	arg1	polysaccharides					2000:2014	cell wall polysaccharides	1990:2014	cell wall polysaccharides	1990:2014	In general, cell wall polysaccharides decreased during softening and tended to be concentrated in the cell corner for most parenchymal cells at the late stage, but there were also some cells not in line with the whole softening trends.					
32244921	0	73	from	Multiple-Cell	136:148	arg1	Peach					115:119	Peach	115:119	Peach	115:119	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	3	74	theme	multiple-cell	658:670	arg1	levels					672:677	both single- and multiple-cell levels	641:677	both single- and multiple-cell levels in a label-free way	641:697	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	0	75	from	Single-	124:130	arg1	Peach					115:119	Peach	115:119	Peach	115:119	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	3	76	from	levels	672:677	arg1	way					695:697	a label-free way	682:697	a label-free way	682:697	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	11	77	theme	changes	2490:2496	arg1	visualization					2473:2485	simultaneous visualization	2460:2485	simultaneous visualization of changes in cell wall polysaccharides of peach	2460:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	3	78	theme	label-free	684:693	arg1	way					695:697	a label-free way	682:697	a label-free way	682:697	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	8	79	theme	high-throughput	1785:1799	arg1	measurement					1822:1832	high-throughput cell wall phenotypes measurement	1785:1832	high-throughput cell wall phenotypes measurement	1785:1832	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	11	80	theme	peach	2350:2354	arg1	fruit					2356:2360	peach fruit	2350:2360	peach fruit	2350:2360	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	1	81	theme	compositional	273:285	arg1	changes					299:305	both compositional and spatial changes	268:305	both compositional and spatial changes of polysaccharides within cell wall	268:341	Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall, affects the texture and quality of fruit.					
32244921	7	82	theme	pectin	1429:1434	arg1	changes					1388:1394	the spatial changes	1376:1394	the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh	1376:1466	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	7	83	theme	hemicellulose	1410:1422	arg1	changes					1388:1394	the spatial changes	1376:1394	the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh	1376:1466	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	6	84	theme	fruit	1228:1232	arg1	distribution					1177:1188	the spatial distribution	1165:1188	the spatial distribution of cell wall polysaccharides of peach fruit	1165:1232	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	11	85	from	differences	2247:2257	arg1	content					2266:2272	content	2266:2272	content	2266:2272	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	11	85	from	differences	2247:2257	arg1	changes					2286:2292	spatial changes	2278:2292	spatial changes	2278:2292	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	7	86	theme	cellulose	1399:1407	arg1	changes					1388:1394	the spatial changes	1376:1394	the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh	1376:1466	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	9	87	theme	degradation	1845:1855	arg1	degrees					1857:1863	Different degradation degrees	1835:1863	Different degradation degrees of parenchymal cells during fruit softening	1835:1907	Different degradation degrees of parenchymal cells during fruit softening were found based on the gray-scale statistical analysis of SRS data.					
32244921	3	88	theme	postharvest	710:720	arg1	storage					722:728	the postharvest storage	706:728	the postharvest storage	706:728	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	1	89	theme	fruit	198:202	arg1	Softening					185:193	Softening	185:193	Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall,	185:342	Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall, affects the texture and quality of fruit.					
32244921	7	90	theme	cell	1589:1592	arg1	corner					1594:1599	the cell corner	1585:1599	the cell corner of parenchymal cells	1585:1620	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	8	91	theme	phenotypes	1811:1820	arg1	measurement					1822:1832	high-throughput cell wall phenotypes measurement	1785:1832	high-throughput cell wall phenotypes measurement	1785:1832	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	6	92	used	used	1136:1139	arg2	scattering					1103:1112	stimulated Raman scattering	1086:1112	stimulated Raman scattering	1086:1112	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	92	used	used	1136:1139	arg2	microspectroscopy					1003:1019	confocal Raman microspectroscopy	988:1019	confocal Raman microspectroscopy (CRM)	988:1025	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	92	used	used	1136:1139	arg2	Fourier					1028:1034	Fourier	1028:1034	Fourier transform infrared microspectroscopy (FTIRM)	1028:1079	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	92	used	used	1136:1139	arg2	techniques					969:978	three molecular imaging techniques	945:978	three molecular imaging techniques	945:978	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	9	93	theme	cells	1880:1884	arg1	degrees					1857:1863	Different degradation degrees	1835:1863	Different degradation degrees of parenchymal cells during fruit softening	1835:1907	Different degradation degrees of parenchymal cells during fruit softening were found based on the gray-scale statistical analysis of SRS data.					
32244921	0	94	theme	Levels	150:155	arg1	Polysaccharides					96:110	Cell Wall Polysaccharides	86:110	Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage	86:182	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	3	95	theme	cell	591:594	arg1	polysaccharides					601:615	cell wall polysaccharides	591:615	cell wall polysaccharides	591:615	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	6	96	theme	cell	1193:1196	arg1	polysaccharides					1203:1217	cell wall polysaccharides	1193:1217	cell wall polysaccharides	1193:1217	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	9	97	theme	fruit	1893:1897	arg1	softening					1899:1907	fruit softening	1893:1907	fruit softening	1893:1907	Different degradation degrees of parenchymal cells during fruit softening were found based on the gray-scale statistical analysis of SRS data.					
32244921	0	98	theme	Postharvest	164:174	arg1	Storage					176:182	Postharvest Storage	164:182	Postharvest Storage	164:182	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	8	99	theme	Raman	1681:1685	arg1	technique					1695:1703	an ultrafast Raman imaging technique	1668:1703	an ultrafast Raman imaging technique	1668:1703	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	8	99	theme	Raman	1681:1685	arg1	SRS					1654:1656	SRS	1654:1656	SRS	1654:1656	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	11	100	theme	softening	2373:2381	arg1	process					2383:2389	the softening process	2369:2389	the softening process	2369:2389	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	11	101	theme	SRS	2430:2432	arg1	method					2449:2454	a promising method	2437:2454	a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach	2437:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	11	101	theme	SRS	2430:2432	arg1	use					2407:2409	the hybrid use	2396:2409	the hybrid use of CRM, FTIRM, and SRS	2396:2432	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	7	102	theme	spectral	1342:1349	arg1	information					1351:1361	complementary spectral information	1328:1361	complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level	1328:1508	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	10	103	theme	most	2096:2099	arg1	cells					2113:2117	most parenchymal cells	2096:2117	most parenchymal cells	2096:2117	In general, cell wall polysaccharides decreased during softening and tended to be concentrated in the cell corner for most parenchymal cells at the late stage, but there were also some cells not in line with the whole softening trends.					
32244921	3	104	from	change	553:558	arg1	cells					632:636	peach flesh cells	620:636	peach flesh cells	620:636	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	3	104	from	change	553:558	arg1	distribution					575:586	the spatial distribution	563:586	the spatial distribution of cell wall polysaccharides	563:615	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	3	105	theme	peach	620:624	arg1	cells					632:636	peach flesh cells	620:636	peach flesh cells	620:636	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	4	106	dep	peaches	742:748	arg1	Batsch					769:774	Batsch	769:774	Batsch	769:774	Nonmelting peaches (Prunus persica L. Batsch cv.					
32244921	7	107	theme	wall	1535:1538	arg1	polysaccharides					1540:1554	the cell wall polysaccharides	1526:1554	the cell wall polysaccharides	1526:1554	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	11	108	theme	fruit	2356:2360	arg1	cells					2341:2345	parenchymal cells	2329:2345	parenchymal cells of peach fruit	2329:2360	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	10	109	theme	cell	2080:2083	arg1	corner					2085:2090	the cell corner	2076:2090	the cell corner for most parenchymal cells	2076:2117	In general, cell wall polysaccharides decreased during softening and tended to be concentrated in the cell corner for most parenchymal cells at the late stage, but there were also some cells not in line with the whole softening trends.					
32244921	3	110	theme	polysaccharides	601:615	arg1	distribution					575:586	the spatial distribution	563:586	the spatial distribution of cell wall polysaccharides	563:615	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32244921	5	111	theme	Firmness	852:859	arg1	measurement					861:871	20 °C. Firmness measurement	845:871	20 °C. Firmness measurement	845:871	"Zhonghuashoutao") at commercial maturity were stored at 0 °C and 20 °C. Firmness measurement and chemical analysis were performed at each storage time.					
32244921	7	112	from	changes	1388:1394	arg1	wall					1448:1451	the cell wall	1439:1451	the cell wall of peach flesh	1439:1466	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	6	113	theme	confocal	988:995	arg1	techniques					969:978	three molecular imaging techniques	945:978	three molecular imaging techniques	945:978	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	113	theme	confocal	988:995	arg1	CRM					1022:1024	CRM	1022:1024	CRM	1022:1024	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	113	theme	confocal	988:995	arg1	microspectroscopy					1003:1019	confocal Raman microspectroscopy	988:1019	confocal Raman microspectroscopy (CRM)	988:1025	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	0	114	theme	Wall	91:94	arg1	Polysaccharides					96:110	Cell Wall Polysaccharides	86:110	Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage	86:182	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	2	115	theme	cell	491:494	arg1	level					496:500	the cell level	487:500	the cell level	487:500	Current research on the fruit softening mechanism lacks an understanding of the overall softening at the cell level.					
32244921	9	116	theme	statistical	1944:1954	arg1	analysis					1956:1963	the gray-scale statistical analysis	1929:1963	the gray-scale statistical analysis of SRS data	1929:1975	Different degradation degrees of parenchymal cells during fruit softening were found based on the gray-scale statistical analysis of SRS data.					
32244921	6	117	theme	imaging	961:967	arg1	techniques					969:978	three molecular imaging techniques	945:978	three molecular imaging techniques	945:978	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	6	117	theme	imaging	961:967	arg1	microspectroscopy					1003:1019	confocal Raman microspectroscopy	988:1019	confocal Raman microspectroscopy (CRM)	988:1025	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	11	118	theme	hybrid	2400:2405	arg1	method					2449:2454	a promising method	2437:2454	a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach	2437:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	11	118	theme	hybrid	2400:2405	arg1	use					2407:2409	the hybrid use	2396:2409	the hybrid use of CRM, FTIRM, and SRS	2396:2432	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	5	119	from	maturity	812:819	arg1	"					795:795	"Zhonghuashoutao")	779:796	"Zhonghuashoutao") at commercial maturity	779:819	"Zhonghuashoutao") at commercial maturity were stored at 0 °C and 20 °C. Firmness measurement and chemical analysis were performed at each storage time.					
32244921	8	120	theme	magnitude	1744:1752	arg1	orders					1734:1739	approximately three or four orders	1706:1739	approximately three or four orders of magnitude faster than CRM	1706:1768	Furthermore, SRS, which is an ultrafast Raman imaging technique (approximately three or four orders of magnitude faster than CRM), was used for high-throughput cell wall phenotypes measurement.					
32244921	9	121	theme	data	1972:1975	arg1	analysis					1956:1963	the gray-scale statistical analysis	1929:1963	the gray-scale statistical analysis of SRS data	1929:1975	Different degradation degrees of parenchymal cells during fruit softening were found based on the gray-scale statistical analysis of SRS data.					
32244921	0	122	theme	Label-Free	7:16	arg1	Microscopies					28:39	Hybrid Label-Free Molecular Microscopies	0:39	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.	0:183	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	7	123	theme	late	1629:1632	arg1	stage					1634:1638	the late stage	1625:1638	the late stage	1625:1638	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	1	124	dep	texture	356:362	arg1	the					352:354	the	352:354	the	352:354	Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall, affects the texture and quality of fruit.					
32244921	6	125	dep	Fourier	1028:1034	arg1	transform					1036:1044	transform	1036:1044	transform infrared microspectroscopy (FTIRM)	1036:1079	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	2	126	theme	Current	386:392	arg1	research					394:401	Current research	386:401	Current research on the fruit softening mechanism	386:434	Current research on the fruit softening mechanism lacks an understanding of the overall softening at the cell level.					
32244921	5	127	theme	commercial	801:810	arg1	maturity					812:819	commercial maturity	801:819	commercial maturity	801:819	"Zhonghuashoutao") at commercial maturity were stored at 0 °C and 20 °C. Firmness measurement and chemical analysis were performed at each storage time.					
32244921	0	128	theme	Simultaneous	45:56	arg1	Visualization					58:70	Simultaneous Visualization	45:70	Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage	45:182	Hybrid Label-Free Molecular Microscopies for Simultaneous Visualization of Changes in Cell Wall Polysaccharides of Peach at Single- and Multiple-Cell Levels during Postharvest Storage.					
32244921	1	129	theme	polysaccharides	310:324	arg1	changes					299:305	both compositional and spatial changes	268:305	both compositional and spatial changes of polysaccharides within cell wall	268:341	Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall, affects the texture and quality of fruit.					
32244921	7	130	theme	parenchymal	1604:1614	arg1	cells					1616:1620	parenchymal cells	1604:1620	parenchymal cells	1604:1620	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	2	131	theme	fruit	410:414	arg1	mechanism					426:434	the fruit softening mechanism	406:434	the fruit softening mechanism	406:434	Current research on the fruit softening mechanism lacks an understanding of the overall softening at the cell level.					
32244921	5	132	theme	Zhonghuashoutao	780:794	arg1	"					795:795	"Zhonghuashoutao")	779:796	"Zhonghuashoutao") at commercial maturity	779:819	"Zhonghuashoutao") at commercial maturity were stored at 0 °C and 20 °C. Firmness measurement and chemical analysis were performed at each storage time.					
32244921	1	133	theme	cell	333:336	arg1	wall					338:341	cell wall	333:341	cell wall	333:341	Softening of fruit during the postharvest storage, which is mainly associated with both compositional and spatial changes of polysaccharides within cell wall, affects the texture and quality of fruit.					
32244921	2	134	theme	overall	466:472	arg1	softening					474:482	the overall softening	462:482	the overall softening	462:482	Current research on the fruit softening mechanism lacks an understanding of the overall softening at the cell level.					
32244921	11	135	theme	cell	2297:2300	arg1	polysaccharides					2307:2321	cell wall polysaccharides	2297:2321	cell wall polysaccharides	2297:2321	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	6	136	theme	stimulated	1086:1095	arg1	scattering					1103:1112	stimulated Raman scattering	1086:1112	stimulated Raman scattering	1086:1112	In addition, three molecular imaging techniques, namely confocal Raman microspectroscopy (CRM), Fourier transform infrared microspectroscopy (FTIRM), and stimulated Raman scattering microscopy (SRS) were used to visualize changes in the spatial distribution of cell wall polysaccharides of peach fruit in a label-free way during the postharvest storage.					
32244921	10	137	theme	wall	1995:1998	arg1	polysaccharides					2000:2014	cell wall polysaccharides	1990:2014	cell wall polysaccharides	1990:2014	In general, cell wall polysaccharides decreased during softening and tended to be concentrated in the cell corner for most parenchymal cells at the late stage, but there were also some cells not in line with the whole softening trends.					
32244921	11	138	theme	parenchymal	2329:2339	arg1	cells					2341:2345	parenchymal cells	2329:2345	parenchymal cells of peach fruit	2329:2360	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	7	139	theme	flesh	1462:1466	arg1	wall					1448:1451	the cell wall	1439:1451	the cell wall of peach flesh	1439:1466	The combination of CRM and FTIRM provided complementary spectral information to visualize the spatial changes of cellulose, hemicellulose, and pectin in the cell wall of peach flesh during softening at the single-cell level, and found that the cell wall polysaccharides tended to be concentrated in the cell corner of parenchymal cells at the late stage.					
32244921	11	140	theme	promising	2439:2447	arg1	method					2449:2454	a promising method	2437:2454	a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach	2437:2534	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	11	140	theme	promising	2439:2447	arg1	use					2407:2409	the hybrid use	2396:2409	the hybrid use of CRM, FTIRM, and SRS	2396:2432	The results show that there were differences in the content and spatial changes of cell wall polysaccharides among parenchymal cells of peach fruit during the softening process, and the hybrid use of CRM, FTIRM, and SRS is a promising method for simultaneous visualization of changes in cell wall polysaccharides of peach.					
32244921	3	141	theme	work	525:528	arg1	objective					507:515	The objective	503:515	The objective of this work	503:528	The objective of this work was to investigate the change in the spatial distribution of cell wall polysaccharides in peach flesh cells at both single- and multiple-cell levels in a label-free way during the postharvest storage.					
32607986	0	0	theme	Cold	72:75	arg1	Acclimation					77:87	Cold Acclimation	72:87	Cold Acclimation	72:87	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	0	1	from	Changes	12:18	arg1	Structure					55:63	Structure	55:63	Structure	55:63	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	0	1	from	Changes	12:18	arg1	Composition					39:49	Plant Cell Wall Composition	23:49	Plant Cell Wall Composition	23:49	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	6	2	theme	chromatography-mass	1093:1111	arg1	spectrometry					1113:1124	gas chromatography-mass spectrometry	1089:1124	gas chromatography-mass spectrometry (GC-MS)	1089:1132	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	6	2	theme	chromatography-mass	1093:1111	arg1	GC-MS					1127:1131	GC-MS	1127:1131	GC-MS	1127:1131	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	6	3	theme	derivatives	1169:1179	arg1	isolation					1140:1148	isolation	1140:1148	isolation of alditol acetate derivatives of the sugars	1140:1193	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	1	4	contain	has	104:106	arg1	wall					99:102	The cell wall	90:102	The cell wall	90:102	The cell wall has a crucial influence on the mechanical properties of plant cells.					
32607986	1	4	contain	has	104:106	arg2	influence					118:126	a crucial influence	108:126	a crucial influence	108:126	The cell wall has a crucial influence on the mechanical properties of plant cells.					
32607986	1	5	theme	mechanical	135:144	arg1	properties					146:155	the mechanical properties	131:155	the mechanical properties of plant cells	131:170	The cell wall has a crucial influence on the mechanical properties of plant cells.					
32607986	0	6	from	Analysis	0:7	arg1	Structure					55:63	Structure	55:63	Structure	55:63	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	0	6	from	Analysis	0:7	arg1	Composition					39:49	Plant Cell Wall Composition	23:49	Plant Cell Wall Composition	23:49	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	1	7	theme	cell	94:97	arg1	wall					99:102	The cell wall	90:102	The cell wall	90:102	The cell wall has a crucial influence on the mechanical properties of plant cells.					
32607986	5	8	from	structure	754:762	arg1	material					855:862	the total cell wall material	835:862	the total cell wall material	835:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	5	9	theme	cold	897:900	arg1	acclimation					902:912	cold acclimation	897:912	cold acclimation	897:912	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	0	10	from	Composition	39:49	arg1	Analysis					0:7	Analysis	0:7	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.	0:88	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	6	11	theme	material	950:957	arg1	properties					926:935	Structural properties	915:935	Structural properties of cell wall material	915:957	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	6	12	theme	sugars	1188:1193	arg1	derivatives					1169:1179	alditol acetate derivatives	1153:1179	alditol acetate derivatives of the sugars	1153:1193	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	4	13	theme	Arabidopsis	539:549	arg1	thaliana					551:558	Arabidopsis thaliana	539:558	Arabidopsis thaliana	539:558	In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.					
32607986	3	14	theme	many	299:302	arg1	studies					304:310	not many studies	295:310	not many studies	295:310	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	5	15	from	material	855:862	arg1	analysis					733:740	the analysis	729:740	the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material	729:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	3	16	theme	impact	380:385	arg1	tolerance					402:410	impact plant freezing tolerance	380:410	impact plant freezing tolerance	380:410	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	3	17	theme	freezing	393:400	arg1	tolerance					402:410	impact plant freezing tolerance	380:410	impact plant freezing tolerance	380:410	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	4	18	from	leaves	529:534	arg1	material					515:522	extract total cell wall material	491:522	extract total cell wall material from leaves of Arabidopsis thaliana	491:558	In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.					
32607986	5	19	from	composition	780:790	arg1	material					855:862	the total cell wall material	835:862	the total cell wall material	835:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	3	20	dep	composition	354:364	arg1	tolerance					402:410	impact plant freezing tolerance	380:410	impact plant freezing tolerance	380:410	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	3	20	dep	composition	354:364	arg1	acclimation					421:431	cold acclimation	416:431	cold acclimation	416:431	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	6	21	theme	gas	1089:1091	arg1	spectrometry					1113:1124	gas chromatography-mass spectrometry	1089:1124	gas chromatography-mass spectrometry (GC-MS)	1089:1132	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	6	21	theme	gas	1089:1091	arg1	GC-MS					1127:1131	GC-MS	1127:1131	GC-MS	1127:1131	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	5	22	theme	composition	780:790	arg1	analysis					733:740	the analysis	729:740	the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material	729:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	5	23	theme	contents	823:830	arg1	analysis					733:740	the analysis	729:740	the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material	729:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	6	24	theme	wall	945:948	arg1	material					950:957	cell wall material	940:957	cell wall material	940:957	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	2	25	theme	plants	278:283	arg1	tolerance					265:273	the freezing tolerance	252:273	the freezing tolerance of plants	252:283	It therefore has a strong impact on the freezing behavior and very likely also the freezing tolerance of plants.					
32607986	6	26	theme	monosaccharide	1059:1072	arg1	composition					1074:1084	monosaccharide composition	1059:1084	monosaccharide composition	1059:1084	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	1	27	theme	plant	160:164	arg1	cells					166:170	plant cells	160:170	plant cells	160:170	The cell wall has a crucial influence on the mechanical properties of plant cells.					
32607986	3	28	theme	cell	344:347	arg1	composition					354:364	cell wall composition	344:364	cell wall composition	344:364	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	4	29	theme	wall	510:513	arg1	material					515:522	extract total cell wall material	491:522	extract total cell wall material from leaves of Arabidopsis thaliana	491:558	In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.					
32607986	4	30	theme	crystalline	617:627	arg1	cellulose					629:637	crystalline cellulose	617:637	crystalline cellulose	617:637	In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.					
32607986	6	31	theme	acetate	1161:1167	arg1	derivatives					1169:1179	alditol acetate derivatives	1153:1179	alditol acetate derivatives of the sugars	1153:1193	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	1	32	theme	cells	166:170	arg1	properties					146:155	the mechanical properties	131:155	the mechanical properties of plant cells	131:170	The cell wall has a crucial influence on the mechanical properties of plant cells.					
32607986	4	33	theme	cell	505:508	arg1	material					515:522	extract total cell wall material	491:522	extract total cell wall material from leaves of Arabidopsis thaliana	491:558	In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.					
32607986	5	34	theme	chemical	745:752	arg1	structure					754:762	chemical structure	745:762	chemical structure	745:762	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	5	35	from	fractions	872:880	arg1	response					885:892	response	885:892	response to cold acclimation	885:912	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	0	36	theme	Changes	12:18	arg1	Analysis					0:7	Analysis	0:7	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.	0:88	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	6	37	dep	spectrometry	1031:1042	arg1	ATR-FTIR					1045:1052	ATR-FTIR	1045:1052	ATR-FTIR	1045:1052	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	2	38	theme	freezing	256:263	arg1	tolerance					265:273	the freezing tolerance	252:273	the freezing tolerance of plants	252:283	It therefore has a strong impact on the freezing behavior and very likely also the freezing tolerance of plants.					
32607986	5	39	theme	uronic	811:816	arg1	contents					823:830	cellulose and uronic acid contents	797:830	cellulose and uronic acid contents in the total cell wall material	797:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	0	40	theme	Cell	29:32	arg1	Composition					39:49	Plant Cell Wall Composition	23:49	Plant Cell Wall Composition	23:49	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	5	41	from	methods	717:723	arg1	response					885:892	response	885:892	response to cold acclimation	885:912	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	3	42	theme	plant	387:391	arg1	tolerance					402:410	impact plant freezing tolerance	380:410	impact plant freezing tolerance	380:410	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	5	43	theme	acid	818:821	arg1	contents					823:830	cellulose and uronic acid contents	797:830	cellulose and uronic acid contents in the total cell wall material	797:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	0	44	theme	Plant	23:27	arg1	Composition					39:49	Plant Cell Wall Composition	23:49	Plant Cell Wall Composition	23:49	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	6	45	theme	Structural	915:924	arg1	properties					926:935	Structural properties	915:935	Structural properties of cell wall material	915:957	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	5	46	theme	structure	754:762	arg1	analysis					733:740	the analysis	729:740	the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material	729:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	0	47	from	Structure	55:63	arg1	Analysis					0:7	Analysis	0:7	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.	0:88	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	4	48	theme	comprehensive	465:477	arg1	workflow					479:486	a comprehensive workflow	463:486	a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana	463:558	In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.					
32607986	2	49	contain	has	186:188	arg1	It					173:174	It	173:174	It	173:174	It therefore has a strong impact on the freezing behavior and very likely also the freezing tolerance of plants.					
32607986	2	49	contain	has	186:188	arg2	tolerance					265:273	the freezing tolerance	252:273	the freezing tolerance of plants	252:283	It therefore has a strong impact on the freezing behavior and very likely also the freezing tolerance of plants.					
32607986	2	49	contain	has	186:188	arg2	impact					199:204	a strong impact	190:204	a strong impact	190:204	It therefore has a strong impact on the freezing behavior and very likely also the freezing tolerance of plants.					
32607986	3	50	theme	wall	349:352	arg1	composition					354:364	cell wall composition	344:364	cell wall composition	344:364	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	5	51	theme	total	839:843	arg1	material					855:862	the total cell wall material	835:862	the total cell wall material	835:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	5	52	theme	monosaccharide	765:778	arg1	composition					780:790	monosaccharide composition	765:790	monosaccharide composition	765:790	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	6	53	theme	cell	940:943	arg1	material					950:957	cell wall material	940:957	cell wall material	940:957	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	2	54	theme	strong	192:197	arg1	impact					199:204	a strong impact	190:204	a strong impact	190:204	It therefore has a strong impact on the freezing behavior and very likely also the freezing tolerance of plants.					
32607986	5	55	from	contents	823:830	arg1	material					855:862	the total cell wall material	835:862	the total cell wall material	835:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	4	56	theme	sequential	671:680	arg1	fractionation					682:694	sequential fractionation	671:694	sequential fractionation	671:694	In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.					
32607986	0	57	theme	Wall	34:37	arg1	Composition					39:49	Plant Cell Wall Composition	23:49	Plant Cell Wall Composition	23:49	Analysis of Changes in Plant Cell Wall Composition and Structure During Cold Acclimation.					
32607986	4	58	theme	total	499:503	arg1	material					515:522	extract total cell wall material	491:522	extract total cell wall material from leaves of Arabidopsis thaliana	491:558	In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.					
32607986	5	59	from	analysis	733:740	arg1	material					855:862	the total cell wall material	835:862	the total cell wall material	835:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	6	60	theme	alditol	1153:1159	arg1	derivatives					1169:1179	alditol acetate derivatives	1153:1179	alditol acetate derivatives of the sugars	1153:1193	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	4	61	theme	extract	491:497	arg1	material					515:522	extract total cell wall material	491:522	extract total cell wall material from leaves of Arabidopsis thaliana	491:558	In this chapter, we describe a comprehensive workflow to extract total cell wall material from leaves of Arabidopsis thaliana and to separate this material into fractions enriched in crystalline cellulose, pectins, and hemicelluloses by sequential fractionation.					
32607986	5	62	theme	cell	845:848	arg1	material					855:862	the total cell wall material	835:862	the total cell wall material	835:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	6	63	theme	infrared	1022:1029	arg1	spectrometry					1031:1042	attenuated total reflectance-Fourier-transform infrared spectrometry	975:1042	attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR)	975:1053	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	3	64	dep	addressed	317:325	arg1	composition					354:364	cell wall composition	344:364	cell wall composition	344:364	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	3	64	dep	addressed	317:325	arg1	structure					370:378	structure	370:378	structure	370:378	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	1	65	theme	crucial	110:116	arg1	influence					118:126	a crucial influence	108:126	a crucial influence	108:126	The cell wall has a crucial influence on the mechanical properties of plant cells.					
32607986	6	66	theme	reflectance-Fourier-transform	992:1020	arg1	spectrometry					1031:1042	attenuated total reflectance-Fourier-transform infrared spectrometry	975:1042	attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR)	975:1053	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	3	67	theme	cold	416:419	arg1	acclimation					421:431	cold acclimation	416:431	cold acclimation	416:431	However, not many studies have addressed the question how cell wall composition and structure impact plant freezing tolerance and cold acclimation.					
32607986	2	68	theme	freezing	213:220	arg1	behavior					222:229	the freezing behavior	209:229	the freezing behavior	209:229	It therefore has a strong impact on the freezing behavior and very likely also the freezing tolerance of plants.					
32607986	5	69	theme	wall	850:853	arg1	material					855:862	the total cell wall material	835:862	the total cell wall material	835:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	6	70	theme	total	986:990	arg1	spectrometry					1031:1042	attenuated total reflectance-Fourier-transform infrared spectrometry	975:1042	attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR)	975:1053	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
32607986	5	71	theme	cellulose	797:805	arg1	contents					823:830	cellulose and uronic acid contents	797:830	cellulose and uronic acid contents in the total cell wall material	797:862	We further describe methods for the analysis of chemical structure, monosaccharide composition, and cellulose and uronic acid contents in the total cell wall material and the fractions in response to cold acclimation.					
32607986	6	72	theme	attenuated	975:984	arg1	spectrometry					1031:1042	attenuated total reflectance-Fourier-transform infrared spectrometry	975:1042	attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR)	975:1053	Structural properties of cell wall material are analyzed by attenuated total reflectance-Fourier-transform infrared spectrometry (ATR-FTIR) and monosaccharide composition by gas chromatography-mass spectrometry (GC-MS) after isolation of alditol acetate derivatives of the sugars.					
33050246	3	0	theme	l-N-acetylguluronic	680:698	arg1	acid					700:703	l-N-acetylguluronic acid	680:703	l-N-acetylguluronic acid	680:703	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	3	0	theme	l-N-acetylguluronic	680:698	arg1	residues					614:621	three residues	608:621	three residues	608:621	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	2	1	theme	skin	336:339	arg1	functions					319:327	the physical and chemical barrier functions	285:327	the physical and chemical barrier functions of the skin	285:339	It is appreciated for its ability to improve the physical and chemical barrier functions of the skin by notably increasing the keratinocyte differentiation and epidermal renewal.					
33050246	4	2	theme	-α-d-Gal-	845:853	arg1	1→					907:908	1→	907:908	1→	907:908	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	4	2	theme	-α-d-Gal-	845:853	arg1	-β-d-GlcNAc-					894:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-	820:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→]	820:909	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	3	3	theme	depth	447:451	arg1	characterization					453:468	depth characterization	447:468	depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses	447:552	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	4	4	theme	unit	835:838	arg1	1→					907:908	1→	907:908	1→	907:908	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	4	4	theme	unit	835:838	arg1	-β-d-GlcNAc-					894:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-	820:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→]	820:909	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	2	5	theme	chemical	302:309	arg1	functions					319:327	the physical and chemical barrier functions	285:327	the physical and chemical barrier functions of the skin	285:339	It is appreciated for its ability to improve the physical and chemical barrier functions of the skin by notably increasing the keratinocyte differentiation and epidermal renewal.					
33050246	2	6	theme	keratinocyte	367:378	arg1	differentiation					380:394	keratinocyte differentiation	367:394	keratinocyte differentiation	367:394	It is appreciated for its ability to improve the physical and chemical barrier functions of the skin by notably increasing the keratinocyte differentiation and epidermal renewal.					
33050246	4	7	theme	repetition	824:833	arg1	1→					907:908	1→	907:908	1→	907:908	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	4	7	theme	repetition	824:833	arg1	-β-d-GlcNAc-					894:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-	820:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→]	820:909	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	0	8	theme	Polysaccharide	17:30	arg1	Structure					0:8	Structure	0:8	Structure of the Polysaccharide	0:30	Structure of the Polysaccharide Secreted by Vibrio alginolyticus CNCM I-5035 (Epidermist 4.0TM).					
33050246	4	9	theme	1→4	890:892	arg1	1→					907:908	1→	907:908	1→	907:908	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	4	9	theme	1→4	890:892	arg1	-β-d-GlcNAc-					894:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-	820:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→]	820:909	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	2	10	theme	physical	289:296	arg1	functions					319:327	the physical and chemical barrier functions	285:327	the physical and chemical barrier functions of the skin	285:339	It is appreciated for its ability to improve the physical and chemical barrier functions of the skin by notably increasing the keratinocyte differentiation and epidermal renewal.					
33050246	3	11	dep	residues	614:621	arg1	d-Gal					637:641	d-Gal	637:641	d-Gal	637:641	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	3	11	dep	residues	614:621	arg1	d-N-acetylglucosamine					645:665	d-N-acetylglucosamine	645:665	d-N-acetylglucosamine (GlcNAc)	645:674	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	3	11	dep	residues	614:621	arg1	acid					700:703	l-N-acetylguluronic acid	680:703	l-N-acetylguluronic acid	680:703	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	3	11	dep	residues	614:621	arg1	residues					614:621	three residues	608:621	three residues	608:621	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	3	11	dep	residues	614:621	arg1	d-galactose					624:634	d-galactose	624:634	d-galactose (d-Gal)	624:642	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	3	11	dep	residues	614:621	arg1	GlcNAc					668:673	GlcNAc	668:673	GlcNAc	668:673	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	1	12	theme	Vibrio	97:102	arg1	alginolyticus					104:116	Vibrio alginolyticus	97:116	Vibrio alginolyticus (CNCM I-5035)	97:130	Vibrio alginolyticus (CNCM I-5035) secretes an exopolysaccharide used as ingredient in cosmetic industry under the trademark Epidermist 4.0TM.					
33050246	1	12	theme	Vibrio	97:102	arg1	I-5035					124:129	CNCM I-5035	119:129	CNCM I-5035	119:129	Vibrio alginolyticus (CNCM I-5035) secretes an exopolysaccharide used as ingredient in cosmetic industry under the trademark Epidermist 4.0TM.					
33050246	3	13	theme	polysaccharides	477:491	arg1	characterization					453:468	depth characterization	447:468	depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses	447:552	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	4	14	theme	polysaccharide	785:798	arg1	structure					768:776	The complete structure	755:776	The complete structure of the polysaccharide	755:798	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	3	15	theme	Composition	419:429	arg1	analyses					431:438	Composition analyses	419:438	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses	419:552	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	2	16	theme	epidermal	400:408	arg1	renewal					410:416	epidermal renewal	400:416	epidermal renewal	400:416	It is appreciated for its ability to improve the physical and chemical barrier functions of the skin by notably increasing the keratinocyte differentiation and epidermal renewal.					
33050246	4	17	theme	1→4	855:857	arg1	1→					907:908	1→	907:908	1→	907:908	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	4	17	theme	1→4	855:857	arg1	-β-d-GlcNAc-					894:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-	820:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→]	820:909	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	3	18	theme	repetition	589:598	arg1	unit					600:603	a repetition unit	587:603	a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3	587:752	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	3	19	theme	oligosaccharides	504:519	arg1	characterization					453:468	depth characterization	447:468	depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses	447:552	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	4	20	theme	-α-l-GulNAcA/α-l-3OAc-GulNAcA-	859:888	arg1	1→					907:908	1→	907:908	1→	907:908	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	4	20	theme	-α-l-GulNAcA/α-l-3OAc-GulNAcA-	859:888	arg1	-β-d-GlcNAc-					894:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-	820:905	the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→]	820:909	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	4	21	theme	complete	759:766	arg1	structure					768:776	The complete structure	755:776	The complete structure of the polysaccharide	755:798	The complete structure of the polysaccharide was resolved giving the repetition unit: [→3)-α-d-Gal-(1→4)-α-l-GulNAcA/α-l-3OAc-GulNAcA-(1→4)-β-d-GlcNAc-(1→].					
33050246	1	22	theme	trademark	212:220	arg1	4.0TM					233:237	the trademark Epidermist 4.0TM	208:237	the trademark Epidermist 4.0TM	208:237	Vibrio alginolyticus (CNCM I-5035) secretes an exopolysaccharide used as ingredient in cosmetic industry under the trademark Epidermist 4.0TM.					
33050246	3	23	from	analyses	431:438	arg1	characterization					453:468	depth characterization	447:468	depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses	447:552	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	2	24	theme	barrier	311:317	arg1	functions					319:327	the physical and chemical barrier functions	285:327	the physical and chemical barrier functions of the skin	285:339	It is appreciated for its ability to improve the physical and chemical barrier functions of the skin by notably increasing the keratinocyte differentiation and epidermal renewal.					
33050246	1	25	theme	Epidermist	222:231	arg1	4.0TM					233:237	the trademark Epidermist 4.0TM	208:237	the trademark Epidermist 4.0TM	208:237	Vibrio alginolyticus (CNCM I-5035) secretes an exopolysaccharide used as ingredient in cosmetic industry under the trademark Epidermist 4.0TM.					
33050246	3	26	theme	residues	614:621	arg1	unit					600:603	a repetition unit	587:603	a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3	587:752	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	1	27	theme	cosmetic	184:191	arg1	industry					193:200	cosmetic industry	184:200	cosmetic industry under the trademark Epidermist 4.0TM	184:237	Vibrio alginolyticus (CNCM I-5035) secretes an exopolysaccharide used as ingredient in cosmetic industry under the trademark Epidermist 4.0TM.					
33050246	3	28	theme	mild	533:536	arg1	hydrolyses					543:552	mild acid hydrolyses	533:552	mild acid hydrolyses	533:552	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
33050246	3	29	theme	acid	538:541	arg1	hydrolyses					543:552	mild acid hydrolyses	533:552	mild acid hydrolyses	533:552	Composition analyses and in depth characterization of the polysaccharides as well as oligosaccharides obtained by mild acid hydrolyses revealed that it was composed of a repetition unit of three residues: d-galactose (d-Gal), d-N-acetylglucosamine (GlcNAc) and l-N-acetylguluronic acid, of which 30% (M/M) was acetylated in position 3.					
35008746	4	0	theme	swelling	841:848	arg1	capacity					831:838	a high swelling capacity	815:838	a high swelling capacity (swelling ratio above 2000%)	815:867	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	0	theme	swelling	841:848	arg1	%					866:866	swelling ratio above 2000%	841:866	swelling ratio above 2000%	841:866	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	1	with	porous	803:808	arg1	capacity					831:838	a high swelling capacity	815:838	a high swelling capacity (swelling ratio above 2000%)	815:867	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	1	with	porous	803:808	arg1	%					866:866	swelling ratio above 2000%	841:866	swelling ratio above 2000%	841:866	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	7	2	theme	observable	1362:1371	arg1	delay					1373:1377	an observable delay	1359:1377	an observable delay in wound healing	1359:1394	All materials, except GG/Alg + NP_NSN, were cytocompatible with L929 fibroblasts and did not cause an observable delay in wound healing.					
35008746	2	3	theme	natural	320:326	arg1	polysaccharides					328:342	natural polysaccharides	320:342	natural polysaccharides	320:342	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	8	4	theme	developed	1417:1425	arg1	materials					1427:1435	the developed materials	1413:1435	the developed materials	1413:1435	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	8	4	theme	developed	1417:1425	arg1	promising					1441:1449	promising	1441:1449	promising	1441:1449	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	7	5	from	delay	1373:1377	arg1	healing					1388:1394	wound healing	1382:1394	wound healing	1382:1394	All materials, except GG/Alg + NP_NSN, were cytocompatible with L929 fibroblasts and did not cause an observable delay in wound healing.					
35008746	4	6	theme	free	710:713	arg1	NSN					715:717	free NSN	710:717	free NSN (GG + NSN and GG/Alg + NSN)	710:745	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	8	7	from	infections	1512:1521	arg1	wounds					1526:1531	wounds	1526:1531	wounds	1526:1531	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	8	8	from	application	1469:1479	arg1	wounds					1526:1531	wounds	1526:1531	wounds	1526:1531	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	4	9	theme	GG	670:671	arg1	sponges					684:690	GG and GG/Alg sponges	670:690	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN)	670:789	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	2	10	theme	antibacterial	453:465	arg1	NSN					482:484	NSN	482:484	NSN	482:484	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	2	10	theme	antibacterial	453:465	arg1	peptide-nisin					467:479	antibacterial peptide-nisin	453:479	antibacterial peptide-nisin (NSN)	453:485	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	4	11	theme	GG/Alg	677:682	arg1	sponges					684:690	GG and GG/Alg sponges	670:690	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN)	670:789	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	2	12	theme	research	260:267	arg1	aim					248:250	The aim	244:250	The aim of this research	244:267	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	8	13	theme	infections	1512:1521	arg1	application					1469:1479	wound healing application	1455:1479	wound healing application	1455:1479	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	8	13	theme	infections	1512:1521	arg1	treatment					1489:1497	the treatment	1485:1497	the treatment of bacterial infections in wounds	1485:1531	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	0	14	theme	Infected	101:108	arg1	Wounds					110:115	Infected Wounds	101:115	Infected Wounds	101:115	Composites Based on Gellan Gum, Alginate and Nisin-Enriched Lipid Nanoparticles for the Treatment of Infected Wounds.					
35008746	5	15	dep	compared	997:1004	arg1	as					994:995	as	994:995	as	994:995	Encapsulation of NSN within lipid nanoparticles significantly slowed down NSN release from GG-based samples for up to 24 h (as compared to GG + NSN).					
35008746	5	16	dep	h	991:991	arg1	compared					997:1004	compared	997:1004	compared to GG + NSN	997:1016	Encapsulation of NSN within lipid nanoparticles significantly slowed down NSN release from GG-based samples for up to 24 h (as compared to GG + NSN).					
35008746	2	17	theme	gellan	351:356	arg1	gum					358:360	gellan gum	351:360	gellan gum (GG)	351:365	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	2	17	theme	gellan	351:356	arg1	GG					363:364	GG	363:364	GG	363:364	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	5	18	theme	NSN	944:946	arg1	release					948:954	NSN release	944:954	NSN release from GG-based samples for up to 24 h (as compared to GG + NSN)	944:1017	Encapsulation of NSN within lipid nanoparticles significantly slowed down NSN release from GG-based samples for up to 24 h (as compared to GG + NSN).					
35008746	4	19	dep	NP_NSN	750:755	arg1	NP_NSN					763:768	NP_NSN	763:768	NP_NSN	763:768	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	19	dep	NP_NSN	750:755	arg1	GG					758:759	GG	758:759	GG	758:759	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	5	20	theme	GG-based	961:968	arg1	samples					970:976	GG-based samples	961:976	GG-based samples for up to 24 h (as compared to GG + NSN)	961:1017	Encapsulation of NSN within lipid nanoparticles significantly slowed down NSN release from GG-based samples for up to 24 h (as compared to GG + NSN).					
35008746	4	21	dep	NSN	715:717	arg1	NSN					725:727	NSN	725:727	NSN	725:727	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	21	dep	NSN	715:717	arg1	GG					720:721	GG	720:721	GG	720:721	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	22	theme	high	817:820	arg1	capacity					831:838	a high swelling capacity	815:838	a high swelling capacity (swelling ratio above 2000%)	815:867	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	22	theme	high	817:820	arg1	%					866:866	swelling ratio above 2000%	841:866	swelling ratio above 2000%	841:866	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	6	23	theme	Streptococcus	1084:1096	arg1	pyogenes					1098:1105	Gram-positive Streptococcus pyogenes	1070:1105	Gram-positive Streptococcus pyogenes	1070:1105	The most effective antimicrobial activity against Gram-positive Streptococcus pyogenes was observed for GG + NP_NSN, while in GG/Alg it was decreased by interactions between NSN and Alg, leading to NSN retention within the hydrogel matrix.					
35008746	3	24	with	spherical	546:554	arg1	size					581:584	an average particle size	561:584	an average particle size of around 300 nm	561:601	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	2	25	theme	composite	286:294	arg1	dressing					302:309	a composite wound dressing	284:309	a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN)	284:485	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	1	26	theme	novel	174:178	arg1	methods					180:186	novel methods	174:186	novel methods of treatment of infected wounds	174:218	Due to growing antimicrobial resistance to antibiotics, novel methods of treatment of infected wounds are being searched for.					
35008746	2	27	theme	GG	384:385	arg1	gum					358:360	gellan gum	351:360	gellan gum (GG)	351:365	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	2	27	theme	GG	384:385	arg1	mixture					373:379	a mixture	371:379	a mixture of GG and alginate (GG/Alg)	371:407	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	2	27	theme	GG	384:385	arg1	GG					363:364	GG	363:364	GG	363:364	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	6	28	theme	Gram-positive	1070:1082	arg1	pyogenes					1098:1105	Gram-positive Streptococcus pyogenes	1070:1105	Gram-positive Streptococcus pyogenes	1070:1105	The most effective antimicrobial activity against Gram-positive Streptococcus pyogenes was observed for GG + NP_NSN, while in GG/Alg it was decreased by interactions between NSN and Alg, leading to NSN retention within the hydrogel matrix.					
35008746	8	29	from	treatment	1489:1497	arg1	wounds					1526:1531	wounds	1526:1531	wounds	1526:1531	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	0	30	theme	Gellan	20:25	arg1	Gum					27:29	Gellan Gum	20:29	Gellan Gum	20:29	Composites Based on Gellan Gum, Alginate and Nisin-Enriched Lipid Nanoparticles for the Treatment of Infected Wounds.					
35008746	5	31	dep	24	988:989	arg1	to					985:986	to	985:986	to	985:986	Encapsulation of NSN within lipid nanoparticles significantly slowed down NSN release from GG-based samples for up to 24 h (as compared to GG + NSN).					
35008746	6	32	theme	effective	1029:1037	arg1	activity					1053:1060	The most effective antimicrobial activity	1020:1060	The most effective antimicrobial activity against Gram-positive Streptococcus pyogenes	1020:1105	The most effective antimicrobial activity against Gram-positive Streptococcus pyogenes was observed for GG + NP_NSN, while in GG/Alg it was decreased by interactions between NSN and Alg, leading to NSN retention within the hydrogel matrix.					
35008746	3	33	theme	NSN-loaded	488:497	arg1	NP_NSN					533:538	NP_NSN	533:538	NP_NSN	533:538	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	3	33	theme	NSN-loaded	488:497	arg1	nanoparticles					518:530	NSN-loaded stearic acid-based nanoparticles	488:530	NSN-loaded stearic acid-based nanoparticles (NP_NSN)	488:539	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	0	34	theme	Wounds	110:115	arg1	Treatment					88:96	the Treatment	84:96	the Treatment of Infected Wounds	84:115	Composites Based on Gellan Gum, Alginate and Nisin-Enriched Lipid Nanoparticles for the Treatment of Infected Wounds.					
35008746	3	35	theme	stearic	499:505	arg1	NP_NSN					533:538	NP_NSN	533:538	NP_NSN	533:538	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	3	35	theme	stearic	499:505	arg1	nanoparticles					518:530	NSN-loaded stearic acid-based nanoparticles	488:530	NSN-loaded stearic acid-based nanoparticles (NP_NSN)	488:539	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	1	36	theme	treatment	191:199	arg1	methods					180:186	novel methods	174:186	novel methods of treatment of infected wounds	174:218	Due to growing antimicrobial resistance to antibiotics, novel methods of treatment of infected wounds are being searched for.					
35008746	4	37	theme	above	856:860	arg1	capacity					831:838	a high swelling capacity	815:838	a high swelling capacity (swelling ratio above 2000%)	815:867	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	37	theme	above	856:860	arg1	%					866:866	swelling ratio above 2000%	841:866	swelling ratio above 2000%	841:866	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	7	38	with	cytocompatible	1304:1317	arg1	fibroblasts					1329:1339	L929 fibroblasts	1324:1339	L929 fibroblasts	1324:1339	All materials, except GG/Alg + NP_NSN, were cytocompatible with L929 fibroblasts and did not cause an observable delay in wound healing.					
35008746	8	39	from	wounds	1526:1531	arg1	application					1469:1479	wound healing application	1455:1479	wound healing application	1455:1479	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	8	39	from	wounds	1526:1531	arg1	treatment					1489:1497	the treatment	1485:1497	the treatment of bacterial infections in wounds	1485:1531	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	4	40	theme	ratio	850:854	arg1	capacity					831:838	a high swelling capacity	815:838	a high swelling capacity (swelling ratio above 2000%)	815:867	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	40	theme	ratio	850:854	arg1	%					866:866	swelling ratio above 2000%	841:866	swelling ratio above 2000%	841:866	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	41	contain	containing	692:701	arg1	sponges					684:690	GG and GG/Alg sponges	670:690	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN)	670:789	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	41	contain	containing	692:701	arg2	NP_NSN					750:755	NP_NSN	750:755	NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN)	750:789	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	41	contain	containing	692:701	arg2	NSN					715:717	free NSN	710:717	free NSN (GG + NSN and GG/Alg + NSN)	710:745	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	3	42	dep	500	659:661	arg1	to					656:657	to	656:657	to	656:657	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	3	43	theme	particle	572:579	arg1	size					581:584	an average particle size	561:584	an average particle size of around 300 nm	561:601	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	6	44	theme	hydrogel	1243:1250	arg1	matrix					1252:1257	the hydrogel matrix	1239:1257	the hydrogel matrix	1239:1257	The most effective antimicrobial activity against Gram-positive Streptococcus pyogenes was observed for GG + NP_NSN, while in GG/Alg it was decreased by interactions between NSN and Alg, leading to NSN retention within the hydrogel matrix.					
35008746	1	45	theme	infected	204:211	arg1	wounds					213:218	infected wounds	204:218	infected wounds	204:218	Due to growing antimicrobial resistance to antibiotics, novel methods of treatment of infected wounds are being searched for.					
35008746	6	46	theme	NSN	1218:1220	arg1	retention					1222:1230	NSN retention	1218:1230	NSN retention within the hydrogel matrix	1218:1257	The most effective antimicrobial activity against Gram-positive Streptococcus pyogenes was observed for GG + NP_NSN, while in GG/Alg it was decreased by interactions between NSN and Alg, leading to NSN retention within the hydrogel matrix.					
35008746	0	47	theme	Nisin-Enriched	45:58	arg1	Nanoparticles					66:78	Nisin-Enriched Lipid Nanoparticles	45:78	Nisin-Enriched Lipid Nanoparticles	45:78	Composites Based on Gellan Gum, Alginate and Nisin-Enriched Lipid Nanoparticles for the Treatment of Infected Wounds.					
35008746	6	48	theme	antimicrobial	1039:1051	arg1	activity					1053:1060	The most effective antimicrobial activity	1020:1060	The most effective antimicrobial activity against Gram-positive Streptococcus pyogenes	1020:1105	The most effective antimicrobial activity against Gram-positive Streptococcus pyogenes was observed for GG + NP_NSN, while in GG/Alg it was decreased by interactions between NSN and Alg, leading to NSN retention within the hydrogel matrix.					
35008746	2	49	contain	containing	410:419	arg1	polysaccharides					328:342	natural polysaccharides	320:342	natural polysaccharides	320:342	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	2	49	contain	containing	410:419	arg2	nanoparticles					427:439	lipid nanoparticles	421:439	lipid nanoparticles loaded with antibacterial peptide-nisin (NSN)	421:485	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	2	50	theme	alginate	391:398	arg1	gum					358:360	gellan gum	351:360	gellan gum (GG)	351:365	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	2	50	theme	alginate	391:398	arg1	mixture					373:379	a mixture	371:379	a mixture of GG and alginate (GG/Alg)	371:407	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	2	50	theme	alginate	391:398	arg1	GG					363:364	GG	363:364	GG	363:364	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	5	51	theme	NSN	887:889	arg1	Encapsulation					870:882	Encapsulation	870:882	Encapsulation of NSN within lipid nanoparticles	870:916	Encapsulation of NSN within lipid nanoparticles significantly slowed down NSN release from GG-based samples for up to 24 h (as compared to GG + NSN).					
35008746	4	52	theme	swelling	822:829	arg1	capacity					831:838	a high swelling capacity	815:838	a high swelling capacity (swelling ratio above 2000%)	815:867	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	4	52	theme	swelling	822:829	arg1	%					866:866	swelling ratio above 2000%	841:866	swelling ratio above 2000%	841:866	GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%).					
35008746	8	53	theme	wound	1455:1459	arg1	healing					1461:1467	wound healing	1455:1467	wound healing application	1455:1479	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	3	54	theme	acid-based	507:516	arg1	NP_NSN					533:538	NP_NSN	533:538	NP_NSN	533:538	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	3	54	theme	acid-based	507:516	arg1	nanoparticles					518:530	NSN-loaded stearic acid-based nanoparticles	488:530	NSN-loaded stearic acid-based nanoparticles (NP_NSN)	488:539	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	7	55	theme	L929	1324:1327	arg1	fibroblasts					1329:1339	L929 fibroblasts	1324:1339	L929 fibroblasts	1324:1339	All materials, except GG/Alg + NP_NSN, were cytocompatible with L929 fibroblasts and did not cause an observable delay in wound healing.					
35008746	1	56	theme	wounds	213:218	arg1	treatment					191:199	treatment	191:199	treatment of infected wounds	191:218	Due to growing antimicrobial resistance to antibiotics, novel methods of treatment of infected wounds are being searched for.					
35008746	5	57	theme	lipid	898:902	arg1	nanoparticles					904:916	lipid nanoparticles	898:916	lipid nanoparticles	898:916	Encapsulation of NSN within lipid nanoparticles significantly slowed down NSN release from GG-based samples for up to 24 h (as compared to GG + NSN).					
35008746	3	58	theme	average	564:570	arg1	size					581:584	an average particle size	561:584	an average particle size of around 300 nm	561:601	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	2	59	theme	wound	296:300	arg1	dressing					302:309	a composite wound dressing	284:309	a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN)	284:485	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	8	60	theme	bacterial	1502:1510	arg1	infections					1512:1521	bacterial infections	1502:1521	bacterial infections in wounds	1502:1531	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	0	61	theme	Lipid	60:64	arg1	Nanoparticles					66:78	Nisin-Enriched Lipid Nanoparticles	45:78	Nisin-Enriched Lipid Nanoparticles	45:78	Composites Based on Gellan Gum, Alginate and Nisin-Enriched Lipid Nanoparticles for the Treatment of Infected Wounds.					
35008746	3	62	theme	nm	600:601	arg1	size					581:584	an average particle size	561:584	an average particle size of around 300 nm	561:601	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	5	63	from	samples	970:976	arg1	release					948:954	NSN release	944:954	NSN release from GG-based samples for up to 24 h (as compared to GG + NSN)	944:1017	Encapsulation of NSN within lipid nanoparticles significantly slowed down NSN release from GG-based samples for up to 24 h (as compared to GG + NSN).					
35008746	8	64	theme	healing	1461:1467	arg1	application					1469:1479	wound healing application	1455:1479	wound healing application	1455:1479	We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.					
35008746	2	65	theme	lipid	421:425	arg1	nanoparticles					427:439	lipid nanoparticles	421:439	lipid nanoparticles loaded with antibacterial peptide-nisin (NSN)	421:485	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	3	66	theme	L929	632:635	arg1	fibroblasts					637:647	L929 fibroblasts	632:647	L929 fibroblasts for up to 500 µg/mL	632:667	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	2	67	dep	gum	358:360	arg1	i.e.					345:348	i.e.	345:348	i.e.	345:348	The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide-nisin (NSN).					
35008746	1	68	theme	antimicrobial	133:145	arg1	resistance					147:156	antimicrobial resistance	133:156	antimicrobial resistance to antibiotics	133:171	Due to growing antimicrobial resistance to antibiotics, novel methods of treatment of infected wounds are being searched for.					
35008746	3	69	with	cytocompatible	612:625	arg1	fibroblasts					637:647	L929 fibroblasts	632:647	L929 fibroblasts for up to 500 µg/mL	632:667	NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL.					
35008746	7	70	theme	wound	1382:1386	arg1	healing					1388:1394	wound healing	1382:1394	wound healing	1382:1394	All materials, except GG/Alg + NP_NSN, were cytocompatible with L929 fibroblasts and did not cause an observable delay in wound healing.					
33871776	4	0	theme	host	908:911	arg1	materials					913:921	jute, wool, cotton, and maize starch host materials	871:921	jute, wool, cotton, and maize starch host materials	871:921	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	7	1	theme	composites	1333:1342	arg1	jute/CaCl2/starch					1380:1396	jute/CaCl2/starch	1380:1396	jute/CaCl2/starch	1380:1396	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	7	1	theme	composites	1333:1342	arg1	cotton/CaCl2/starch					1403:1421	cotton/CaCl2/starch	1403:1421	cotton/CaCl2/starch	1403:1421	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	7	1	theme	composites	1333:1342	arg1	wool/CaCl2/starch					1361:1377	wool/CaCl2/starch	1361:1377	wool/CaCl2/starch	1361:1377	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	7	1	theme	composites	1333:1342	arg1	stages					1344:1349	the single hybrid composites stages	1315:1349	the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch	1315:1421	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	7	2	theme	weight	1213:1218	arg1	percentages					1220:1230	weight percentages	1213:1230	weight percentages of hybrid composite desiccant materials	1213:1270	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	3	3	theme	hybrid	547:552	arg1	material					581:588	hybrid single composite desiccant material	547:588	hybrid single composite desiccant material	547:588	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	1	4	theme	near	261:264	arg1	theme					304:308	the near future sustainable cleaner production theme to promote civilization	257:332	the near future sustainable cleaner production theme to promote civilization	257:332	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	8	5	theme	composite	1500:1508	arg1	desiccants					1510:1519	composite desiccants	1500:1519	composite desiccants increased as the wool percentage by mass	1500:1560	Results have indicated that the transient behavior of water productivity of composite desiccants increased as the wool percentage by mass in the composite has been increased.					
33871776	1	6	theme	sustainable	273:283	arg1	theme					304:308	the near future sustainable cleaner production theme to promote civilization	257:332	the near future sustainable cleaner production theme to promote civilization	257:332	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	7	7	theme	Different	1180:1188	arg1	combinations					1196:1207	Different layer combinations	1180:1207	Different layer combinations	1180:1207	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	10	8	theme	excellent	1844:1852	arg1	category					1854:1861	the excellent category	1840:1861	the excellent category of clean water	1840:1876	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	4	9	theme	maize	895:899	arg1	materials					913:921	jute, wool, cotton, and maize starch host materials	871:921	jute, wool, cotton, and maize starch host materials	871:921	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	3	10	theme	desiccant	625:633	arg1	materials					635:643	composite desiccant materials	615:643	composite desiccant materials	615:643	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	1	11	theme	low-cost	199:206	arg1	resources					216:224	available low-cost natural resources	189:224	available low-cost natural resources	189:224	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	10	12	theme	water	1872:1876	arg1	category					1854:1861	the excellent category	1840:1861	the excellent category of clean water	1840:1876	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	9	13	theme	water	1625:1629	arg1	productivity					1631:1642	water productivity	1625:1642	water productivity of both single and hybrid multi-layer composites	1625:1691	The transient behavior of water productivity of both single and hybrid multi-layer composites reached its maximum at 1:00 o'clock PM.					
33871776	10	14	theme	extracted	1748:1756	arg1	water					1758:1762	extracted water	1748:1762	extracted water	1748:1762	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	7	15	from	performance	1300:1310	arg1	jute/CaCl2/starch					1380:1396	jute/CaCl2/starch	1380:1396	jute/CaCl2/starch	1380:1396	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	7	15	from	performance	1300:1310	arg1	cotton/CaCl2/starch					1403:1421	cotton/CaCl2/starch	1403:1421	cotton/CaCl2/starch	1403:1421	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	7	15	from	performance	1300:1310	arg1	wool/CaCl2/starch					1361:1377	wool/CaCl2/starch	1361:1377	wool/CaCl2/starch	1361:1377	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	7	15	from	performance	1300:1310	arg1	stages					1344:1349	the single hybrid composites stages	1315:1349	the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch	1315:1421	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	5	16	theme	concentration	953:965	arg1	effect					928:933	The effect	924:933	The effect of CaCl2 solution concentration on the hybridization of such materials	924:1004	The effect of CaCl2 solution concentration on the hybridization of such materials was also investigated to examine and optimize their water productivity efficiency.					
33871776	4	17	theme	single	766:771	arg1	materials					793:801	Nine different single composite desiccant materials	751:801	Nine different single composite desiccant materials	751:801	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	9	18	theme	1:00	1716:1719	arg1	clock					1723:1727	1:00 o'clock	1716:1727	1:00 o'clock PM	1716:1730	The transient behavior of water productivity of both single and hybrid multi-layer composites reached its maximum at 1:00 o'clock PM.					
33871776	1	19	theme	clean	146:150	arg1	water					152:156	clean water	146:156	clean water	146:156	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	7	20	theme	hybrid	1326:1331	arg1	composites					1333:1342	the single hybrid composites	1315:1342	the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch	1315:1421	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	3	21	theme	efficient	668:676	arg1	design					678:683	an efficient design	665:683	an efficient design of a solar glass box with four glass faces and square base setup	665:748	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	9	22	theme	single	1652:1657	arg1	composites					1682:1691	both single and hybrid multi-layer composites	1647:1691	both single and hybrid multi-layer composites	1647:1691	The transient behavior of water productivity of both single and hybrid multi-layer composites reached its maximum at 1:00 o'clock PM.					
33871776	8	23	theme	transient	1456:1464	arg1	behavior					1466:1473	the transient behavior	1452:1473	the transient behavior of water productivity of composite desiccants increased as the wool percentage by mass in the composite	1452:1577	Results have indicated that the transient behavior of water productivity of composite desiccants increased as the wool percentage by mass in the composite has been increased.					
33871776	1	24	theme	solar	172:176	arg1	energy					178:183	renewable solar energy	162:183	renewable solar energy	162:183	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	9	25	theme	o'	1721:1722	arg1	clock					1723:1727	1:00 o'clock	1716:1727	1:00 o'clock PM	1716:1730	The transient behavior of water productivity of both single and hybrid multi-layer composites reached its maximum at 1:00 o'clock PM.					
33871776	0	26	theme	water	97:101	arg1	extraction					103:112	water extraction	97:112	water extraction from atmospheric air	97:133	Transient behavior of non-toxic natural and hybrid multi-layer desiccant composite materials for water extraction from atmospheric air.					
33871776	9	27	theme	composites	1682:1691	arg1	productivity					1631:1642	water productivity	1625:1642	water productivity of both single and hybrid multi-layer composites	1625:1691	The transient behavior of water productivity of both single and hybrid multi-layer composites reached its maximum at 1:00 o'clock PM.					
33871776	8	28	theme	productivity	1484:1495	arg1	behavior					1466:1473	the transient behavior	1452:1473	the transient behavior of water productivity of composite desiccants increased as the wool percentage by mass in the composite	1452:1577	Results have indicated that the transient behavior of water productivity of composite desiccants increased as the wool percentage by mass in the composite has been increased.					
33871776	2	29	theme	system	422:427	arg1	behavior					372:379	the transient behavior	358:379	the transient behavior of a solar-driven clean water extraction system from air	358:436	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	3	30	dep	materials	536:544	arg1	examined					650:657	examined	650:657	were examined using an efficient design of a solar glass box with four glass faces and square base setup	645:748	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	6	31	theme	material	1150:1157	arg1	types					1159:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types were utilized.					
33871776	7	32	theme	single	1319:1324	arg1	composites					1333:1342	the single hybrid composites	1315:1342	the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch	1315:1421	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	4	33	theme	desiccant	783:791	arg1	materials					793:801	Nine different single composite desiccant materials	751:801	Nine different single composite desiccant materials	751:801	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	2	34	theme	water	405:409	arg1	system					422:427	a solar-driven clean water extraction system	384:427	a solar-driven clean water extraction system	384:427	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	0	35	theme	Transient	0:8	arg1	behavior					10:17	Transient behavior	0:17	Transient behavior of non-toxic natural and hybrid multi-layer	0:61	Transient behavior of non-toxic natural and hybrid multi-layer desiccant composite materials for water extraction from atmospheric air.					
33871776	1	36	theme	cleaner	285:291	arg1	theme					304:308	the near future sustainable cleaner production theme to promote civilization	257:332	the near future sustainable cleaner production theme to promote civilization	257:332	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	6	37	theme	composite	1130:1138	arg1	types					1159:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types were utilized.					
33871776	2	38	theme	natural	459:465	arg1	materials					488:496	various desiccant natural and hybrid composite materials	441:496	various desiccant natural and hybrid composite materials	441:496	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	9	39	theme	clock	1723:1727	arg1	PM					1729:1730	1:00 o'clock PM	1716:1730	1:00 o'clock PM	1716:1730	The transient behavior of water productivity of both single and hybrid multi-layer composites reached its maximum at 1:00 o'clock PM.					
33871776	2	40	theme	various	441:447	arg1	materials					488:496	various desiccant natural and hybrid composite materials	441:496	various desiccant natural and hybrid composite materials	441:496	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	7	41	theme	materials	1262:1270	arg1	combinations					1196:1207	Different layer combinations	1180:1207	Different layer combinations	1180:1207	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	7	41	theme	materials	1262:1270	arg1	percentages					1220:1230	weight percentages	1213:1230	weight percentages of hybrid composite desiccant materials	1213:1270	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	5	42	theme	materials	996:1004	arg1	hybridization					974:986	the hybridization	970:986	the hybridization of such materials	970:1004	The effect of CaCl2 solution concentration on the hybridization of such materials was also investigated to examine and optimize their water productivity efficiency.					
33871776	6	43	theme	multi-layer	1105:1115	arg1	types					1159:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types were utilized.					
33871776	3	44	theme	box	702:704	arg1	design					678:683	an efficient design	665:683	an efficient design of a solar glass box with four glass faces and square base setup	665:748	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	10	45	theme	solids	1799:1804	arg1	test					1812:1815	total dissolved solids (TDS) test	1783:1815	total dissolved solids (TDS) test	1783:1815	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	0	46	theme	natural	32:38	arg1	behavior					10:17	Transient behavior	0:17	Transient behavior of non-toxic natural and hybrid multi-layer	0:61	Transient behavior of non-toxic natural and hybrid multi-layer desiccant composite materials for water extraction from atmospheric air.					
33871776	7	47	theme	composite	1242:1250	arg1	materials					1262:1270	hybrid composite desiccant materials	1235:1270	hybrid composite desiccant materials	1235:1270	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	5	48	theme	productivity	1064:1075	arg1	efficiency					1077:1086	their water productivity efficiency	1052:1086	their water productivity efficiency	1052:1086	The effect of CaCl2 solution concentration on the hybridization of such materials was also investigated to examine and optimize their water productivity efficiency.					
33871776	3	49	theme	solar	690:694	arg1	box					702:704	a solar glass box	688:704	a solar glass box with four glass faces and square base setup	688:748	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	3	50	theme	desiccant	526:534	arg1	materials					536:544	Different single composite desiccant materials	499:544	Different single composite desiccant materials	499:544	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	11	51	contain	contain	1942:1948	arg2	fibers					1963:1968	only natural fibers	1950:1968	only natural fibers	1950:1968	Water generated from the samples that contain only natural fibers and starch was the cleaner and non-toxic.					
33871776	11	51	contain	contain	1942:1948	arg2	starch					1974:1979	starch	1974:1979	starch	1974:1979	Water generated from the samples that contain only natural fibers and starch was the cleaner and non-toxic.					
33871776	11	51	contain	contain	1942:1948	arg1	samples					1929:1935	the samples	1925:1935	the samples that contain only natural fibers and starch	1925:1979	Water generated from the samples that contain only natural fibers and starch was the cleaner and non-toxic.					
33871776	4	52	theme	atmospheric	843:853	arg1	air					855:857	atmospheric air	843:857	atmospheric air considering jute, wool, cotton, and maize starch host materials	843:921	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	3	53	theme	single	509:514	arg1	materials					536:544	Different single composite desiccant materials	499:544	Different single composite desiccant materials	499:544	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	2	54	from	air	434:436	arg1	behavior					372:379	the transient behavior	358:379	the transient behavior of a solar-driven clean water extraction system from air	358:436	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	3	55	theme	single	554:559	arg1	material					581:588	hybrid single composite desiccant material	547:588	hybrid single composite desiccant material	547:588	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	8	56	from	behavior	1466:1473	arg1	composite					1569:1577	the composite	1565:1577	the composite	1565:1577	Results have indicated that the transient behavior of water productivity of composite desiccants increased as the wool percentage by mass in the composite has been increased.					
33871776	1	57	theme	issues	246:251	arg1	issues					246:251	paramount issues	236:251	paramount issues for the near future sustainable cleaner production theme to promote civilization	236:332	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	1	57	theme	issues	246:251	arg1	one					229:231	one	229:231	one	229:231	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	0	58	from	air	131:133	arg1	extraction					103:112	water extraction	97:112	water extraction from atmospheric air	97:133	Transient behavior of non-toxic natural and hybrid multi-layer desiccant composite materials for water extraction from atmospheric air.					
33871776	4	59	theme	starch	901:906	arg1	materials					913:921	jute, wool, cotton, and maize starch host materials	871:921	jute, wool, cotton, and maize starch host materials	871:921	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	3	60	theme	square	732:737	arg1	setup					744:748	square base setup	732:748	square base setup	732:748	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	7	61	theme	layer	1190:1194	arg1	combinations					1196:1207	Different layer combinations	1180:1207	Different layer combinations	1180:1207	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	10	62	theme	clean	1866:1870	arg1	water					1872:1876	clean water	1866:1876	clean water	1866:1876	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	1	63	theme	future	266:271	arg1	theme					304:308	the near future sustainable cleaner production theme to promote civilization	257:332	the near future sustainable cleaner production theme to promote civilization	257:332	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	2	64	theme	transient	362:370	arg1	behavior					372:379	the transient behavior	358:379	the transient behavior of a solar-driven clean water extraction system from air	358:436	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	3	65	theme	desiccant	571:579	arg1	material					581:588	hybrid single composite desiccant material	547:588	hybrid single composite desiccant material	547:588	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	4	66	theme	cotton	883:888	arg1	materials					913:921	jute, wool, cotton, and maize starch host materials	871:921	jute, wool, cotton, and maize starch host materials	871:921	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	3	67	theme	composite	615:623	arg1	materials					635:643	composite desiccant materials	615:643	composite desiccant materials	615:643	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	1	68	theme	available	189:197	arg1	resources					216:224	available low-cost natural resources	189:224	available low-cost natural resources	189:224	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	8	69	theme	wool	1538:1541	arg1	percentage					1543:1552	the wool percentage	1534:1552	the wool percentage	1534:1552	Results have indicated that the transient behavior of water productivity of composite desiccants increased as the wool percentage by mass in the composite has been increased.					
33871776	3	70	theme	hybrid	595:600	arg1	multi-layers					602:613	hybrid multi-layers	595:613	hybrid multi-layers	595:613	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	1	71	theme	natural	208:214	arg1	resources					216:224	available low-cost natural resources	189:224	available low-cost natural resources	189:224	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	2	72	theme	solar-driven	386:397	arg1	system					422:427	a solar-driven clean water extraction system	384:427	a solar-driven clean water extraction system	384:427	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	5	73	theme	CaCl2	938:942	arg1	concentration					953:965	CaCl2 solution concentration	938:965	CaCl2 solution concentration	938:965	The effect of CaCl2 solution concentration on the hybridization of such materials was also investigated to examine and optimize their water productivity efficiency.					
33871776	10	74	theme	water	1758:1762	arg1	suitable					1878:1885	suitable	1878:1885	suitable	1878:1885	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	10	74	theme	water	1758:1762	arg1	quality					1737:1743	The quality	1733:1743	The quality of extracted water	1733:1762	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	11	75	theme	natural	1955:1961	arg1	fibers					1963:1968	only natural fibers	1950:1968	only natural fibers	1950:1968	Water generated from the samples that contain only natural fibers and starch was the cleaner and non-toxic.					
33871776	3	76	with	box	702:704	arg1	setup					744:748	square base setup	732:748	square base setup	732:748	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	3	76	with	box	702:704	arg1	faces					722:726	four glass faces	711:726	four glass faces	711:726	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	9	77	theme	transient	1603:1611	arg1	behavior					1613:1620	The transient behavior	1599:1620	The transient behavior of water productivity of both single and hybrid multi-layer composites	1599:1691	The transient behavior of water productivity of both single and hybrid multi-layer composites reached its maximum at 1:00 o'clock PM.					
33871776	6	78	used	utilized	1170:1177	arg2	types					1159:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types were utilized.					
33871776	5	79	theme	solution	944:951	arg1	concentration					953:965	CaCl2 solution concentration	938:965	CaCl2 solution concentration	938:965	The effect of CaCl2 solution concentration on the hybridization of such materials was also investigated to examine and optimize their water productivity efficiency.					
33871776	9	80	theme	productivity	1631:1642	arg1	behavior					1613:1620	The transient behavior	1599:1620	The transient behavior of water productivity of both single and hybrid multi-layer composites	1599:1691	The transient behavior of water productivity of both single and hybrid multi-layer composites reached its maximum at 1:00 o'clock PM.					
33871776	4	81	theme	different	756:764	arg1	materials					793:801	Nine different single composite desiccant materials	751:801	Nine different single composite desiccant materials	751:801	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	0	82	theme	composite	73:81	arg1	materials					83:91	composite materials	73:91	composite materials for water extraction from atmospheric air	73:133	Transient behavior of non-toxic natural and hybrid multi-layer desiccant composite materials for water extraction from atmospheric air.					
33871776	4	83	from	air	855:857	arg1	production					827:836	water production	821:836	water production from atmospheric air considering jute, wool, cotton, and maize starch host materials	821:921	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	1	84	theme	renewable	162:170	arg1	energy					178:183	renewable solar energy	162:183	renewable solar energy	162:183	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	9	85	theme	multi-layer	1670:1680	arg1	composites					1682:1691	both single and hybrid multi-layer composites	1647:1691	both single and hybrid multi-layer composites	1647:1691	The transient behavior of water productivity of both single and hybrid multi-layer composites reached its maximum at 1:00 o'clock PM.					
33871776	0	86	theme	atmospheric	119:129	arg1	air					131:133	atmospheric air	119:133	atmospheric air	119:133	Transient behavior of non-toxic natural and hybrid multi-layer desiccant composite materials for water extraction from atmospheric air.					
33871776	5	87	from	effect	928:933	arg1	hybridization					974:986	the hybridization	970:986	the hybridization of such materials	970:1004	The effect of CaCl2 solution concentration on the hybridization of such materials was also investigated to examine and optimize their water productivity efficiency.					
33871776	4	88	theme	water	821:825	arg1	production					827:836	water production	821:836	water production from atmospheric air considering jute, wool, cotton, and maize starch host materials	821:921	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	2	89	theme	extraction	411:420	arg1	system					422:427	a solar-driven clean water extraction system	384:427	a solar-driven clean water extraction system	384:427	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	6	90	theme	desiccant	1140:1148	arg1	types					1159:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types were utilized.					
33871776	4	91	theme	composite	773:781	arg1	materials					793:801	Nine different single composite desiccant materials	751:801	Nine different single composite desiccant materials	751:801	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	2	92	theme	clean	399:403	arg1	system					422:427	a solar-driven clean water extraction system	384:427	a solar-driven clean water extraction system	384:427	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	7	93	theme	desiccant	1252:1260	arg1	materials					1262:1270	hybrid composite desiccant materials	1235:1270	hybrid composite desiccant materials	1235:1270	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	1	94	theme	production	293:302	arg1	theme					304:308	the near future sustainable cleaner production theme to promote civilization	257:332	the near future sustainable cleaner production theme to promote civilization	257:332	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	5	95	theme	such	991:994	arg1	materials					996:1004	such materials	991:1004	such materials	991:1004	The effect of CaCl2 solution concentration on the hybridization of such materials was also investigated to examine and optimize their water productivity efficiency.					
33871776	6	96	theme	starch-based	1117:1128	arg1	types					1159:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types were utilized.					
33871776	2	97	theme	desiccant	449:457	arg1	materials					488:496	various desiccant natural and hybrid composite materials	441:496	various desiccant natural and hybrid composite materials	441:496	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	3	98	theme	glass	696:700	arg1	box					702:704	a solar glass box	688:704	a solar glass box with four glass faces and square base setup	688:748	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	8	99	theme	desiccants	1510:1519	arg1	productivity					1484:1495	water productivity	1478:1495	water productivity of composite desiccants increased as the wool percentage by mass	1478:1560	Results have indicated that the transient behavior of water productivity of composite desiccants increased as the wool percentage by mass in the composite has been increased.					
33871776	3	100	theme	Different	499:507	arg1	materials					536:544	Different single composite desiccant materials	499:544	Different single composite desiccant materials	499:544	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	7	101	theme	hybrid	1235:1240	arg1	materials					1262:1270	hybrid composite desiccant materials	1235:1270	hybrid composite desiccant materials	1235:1270	Different layer combinations and weight percentages of hybrid composite desiccant materials were optimized based on the performance in the single hybrid composites stages including wool/CaCl2/starch, jute/CaCl2/starch, and cotton/CaCl2/starch.					
33871776	5	102	theme	water	1058:1062	arg1	efficiency					1077:1086	their water productivity efficiency	1052:1086	their water productivity efficiency	1052:1086	The effect of CaCl2 solution concentration on the hybridization of such materials was also investigated to examine and optimize their water productivity efficiency.					
33871776	9	103	from	PM	1729:1730	arg1	maximum					1705:1711	its maximum	1701:1711	its maximum at 1:00 o'clock PM	1701:1730	The transient behavior of water productivity of both single and hybrid multi-layer composites reached its maximum at 1:00 o'clock PM.					
33871776	6	104	theme	hybrid	1098:1103	arg1	types					1159:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types	1089:1163	Thirteen hybrid multi-layer starch-based composite desiccant material types were utilized.					
33871776	10	105	theme	total	1783:1787	arg1	solids					1799:1804	total dissolved solids	1783:1804	total dissolved solids (TDS) test	1783:1815	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	10	105	theme	total	1783:1787	arg1	TDS					1807:1809	TDS	1807:1809	TDS	1807:1809	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	4	106	theme	jute	871:874	arg1	materials					913:921	jute, wool, cotton, and maize starch host materials	871:921	jute, wool, cotton, and maize starch host materials	871:921	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	10	107	theme	dissolved	1789:1797	arg1	solids					1799:1804	total dissolved solids	1783:1804	total dissolved solids (TDS) test	1783:1815	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	10	107	theme	dissolved	1789:1797	arg1	TDS					1807:1809	TDS	1807:1809	TDS	1807:1809	The quality of extracted water was analyzed using total dissolved solids (TDS) test and found to be within the excellent category of clean water suitable for human being.					
33871776	0	108	theme	multi-layer	51:61	arg1	behavior					10:17	Transient behavior	0:17	Transient behavior of non-toxic natural and hybrid multi-layer	0:61	Transient behavior of non-toxic natural and hybrid multi-layer desiccant composite materials for water extraction from atmospheric air.					
33871776	2	109	theme	composite	478:486	arg1	materials					488:496	various desiccant natural and hybrid composite materials	441:496	various desiccant natural and hybrid composite materials	441:496	This work investigates the transient behavior of a solar-driven clean water extraction system from air by various desiccant natural and hybrid composite materials.					
33871776	3	110	theme	glass	716:720	arg1	faces					722:726	four glass faces	711:726	four glass faces	711:726	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	4	111	theme	wool	877:880	arg1	materials					913:921	jute, wool, cotton, and maize starch host materials	871:921	jute, wool, cotton, and maize starch host materials	871:921	Nine different single composite desiccant materials were compared for water production from atmospheric air considering jute, wool, cotton, and maize starch host materials.					
33871776	8	112	theme	water	1478:1482	arg1	productivity					1484:1495	water productivity	1478:1495	water productivity of composite desiccants increased as the wool percentage by mass	1478:1560	Results have indicated that the transient behavior of water productivity of composite desiccants increased as the wool percentage by mass in the composite has been increased.					
33871776	3	113	theme	composite	516:524	arg1	materials					536:544	Different single composite desiccant materials	499:544	Different single composite desiccant materials	499:544	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	1	114	theme	paramount	236:244	arg1	issues					246:251	paramount issues	236:251	paramount issues for the near future sustainable cleaner production theme to promote civilization	236:332	Producing clean water via renewable solar energy and available low-cost natural resources is one of paramount issues for the near future sustainable cleaner production theme to promote civilization.					
33871776	3	115	theme	base	739:742	arg1	setup					744:748	square base setup	732:748	square base setup	732:748	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33871776	3	116	theme	composite	561:569	arg1	material					581:588	hybrid single composite desiccant material	547:588	hybrid single composite desiccant material	547:588	Different single composite desiccant materials, hybrid single composite desiccant material, and hybrid multi-layers composite desiccant materials were examined using an efficient design of a solar glass box with four glass faces and square base setup.					
33887287	0	0	theme	physicochemical	85:99	arg1	properties					116:125	Structural, physicochemical, and bioactive properties	73:125	Structural, physicochemical, and bioactive properties	73:125	Functional polysaccharide-based film prepared from chitosan and β-acids: Structural, physicochemical, and bioactive properties.					
33887287	5	1	theme	tensile	649:655	arg1	strength					657:664	the tensile strength	645:664	the tensile strength of the films	645:677	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	1	2	theme	antioxidant	166:176	arg1	β-Acids					128:134	β-Acids	128:134	β-Acids	128:134	β-Acids are natural antibacterial and antioxidant ingredients, obtained from supercritical CO2 hop extract.					
33887287	1	2	theme	antioxidant	166:176	arg1	ingredients					178:188	natural antibacterial and antioxidant ingredients	140:188	natural antibacterial and antioxidant ingredients	140:188	β-Acids are natural antibacterial and antioxidant ingredients, obtained from supercritical CO2 hop extract.					
33887287	5	3	theme	films	673:677	arg1	strength					657:664	the tensile strength	645:664	the tensile strength of the films	645:677	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	9	4	theme	active	1235:1240	arg1	packaging					1242:1250	active packaging	1235:1250	active packaging	1235:1250	This study highlights the promising nature of composite film as a desirable alternative for active packaging.					
33887287	8	5	theme	complex	966:972	arg1	films					974:978	the complex films	962:978	the complex films	962:978	In addition, the complex films inhibited the growth of five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes).					
33887287	7	6	theme	Fickian	919:925	arg1	diffusion					927:935	Fickian diffusion	919:935	Fickian diffusion (n < 0.45)	919:946	The release of β-acids from the film followed Fickian diffusion (n < 0.45).					
33887287	0	7	dep	properties	116:125	arg1	film					32:35	Functional polysaccharide-based film	0:35	Functional polysaccharide-based film prepared from chitosan and β-acids	0:70	Functional polysaccharide-based film prepared from chitosan and β-acids: Structural, physicochemical, and bioactive properties.					
33887287	7	8	from	film	905:908	arg1	release					877:883	The release	873:883	The release of β-acids from the film	873:908	The release of β-acids from the film followed Fickian diffusion (n < 0.45).					
33887287	3	9	theme	electron	370:377	arg1	microscopy					379:388	scanning electron microscopy	361:388	scanning electron microscopy (SEM)	361:394	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	3	9	theme	electron	370:377	arg1	SEM					391:393	SEM	391:393	SEM	391:393	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	5	10	theme	Mechanical	610:619	arg1	tests					621:625	Mechanical tests	610:625	Mechanical tests	610:625	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	0	11	theme	bioactive	106:114	arg1	properties					116:125	Structural, physicochemical, and bioactive properties	73:125	Structural, physicochemical, and bioactive properties	73:125	Functional polysaccharide-based film prepared from chitosan and β-acids: Structural, physicochemical, and bioactive properties.					
33887287	6	12	theme	barrier	840:846	arg1	capability					848:857	excellent UV barrier capability	827:857	excellent UV barrier capability	827:857	Interestingly, the chitosan-based films showed excellent UV barrier capability below 400 nm.					
33887287	8	13	dep	pathogens	1020:1028	arg1	monocytogenes					1127:1139	Listeria monocytogenes	1118:1139	Listeria monocytogenes	1118:1139	In addition, the complex films inhibited the growth of five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes).					
33887287	8	13	dep	pathogens	1020:1028	arg1	coli					1043:1046	Escherichia coli	1031:1046	Escherichia coli	1031:1046	In addition, the complex films inhibited the growth of five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes).					
33887287	8	13	dep	pathogens	1020:1028	arg1	aureus					1064:1069	Staphylococcus aureus	1049:1069	Staphylococcus aureus	1049:1069	In addition, the complex films inhibited the growth of five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes).					
33887287	8	13	dep	pathogens	1020:1028	arg1	subtilis					1081:1088	Bacillus subtilis	1072:1088	Bacillus subtilis	1072:1088	In addition, the complex films inhibited the growth of five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes).					
33887287	8	13	dep	pathogens	1020:1028	arg1	enteritidis					1102:1112	Salmonella enteritidis	1091:1112	Salmonella enteritidis	1091:1112	In addition, the complex films inhibited the growth of five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes).					
33887287	7	14	theme	β-acids	888:894	arg1	release					877:883	The release	873:883	The release of β-acids from the film	873:908	The release of β-acids from the film followed Fickian diffusion (n < 0.45).					
33887287	0	15	theme	Functional	0:9	arg1	film					32:35	Functional polysaccharide-based film	0:35	Functional polysaccharide-based film prepared from chitosan and β-acids	0:70	Functional polysaccharide-based film prepared from chitosan and β-acids: Structural, physicochemical, and bioactive properties.					
33887287	5	16	theme	9.6	725:727	arg1	MPa					718:720	MPa	718:720	MPa	718:720	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	0	17	theme	polysaccharide-based	11:30	arg1	film					32:35	Functional polysaccharide-based film	0:35	Functional polysaccharide-based film prepared from chitosan and β-acids	0:70	Functional polysaccharide-based film prepared from chitosan and β-acids: Structural, physicochemical, and bioactive properties.					
33887287	6	18	theme	UV	837:838	arg1	capability					848:857	excellent UV barrier capability	827:857	excellent UV barrier capability	827:857	Interestingly, the chitosan-based films showed excellent UV barrier capability below 400 nm.					
33887287	3	19	theme	force	404:408	arg1	AFM					422:424	AFM	422:424	AFM	422:424	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	3	19	theme	force	404:408	arg1	microscope					410:419	atomic force microscope	397:419	atomic force microscope (AFM)	397:425	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	9	20	theme	composite	1189:1197	arg1	film					1199:1202	composite film	1189:1202	composite film	1189:1202	This study highlights the promising nature of composite film as a desirable alternative for active packaging.					
33887287	7	21	dep	diffusion	927:935	arg1	n					938:938	n	938:938	n < 0.45	938:945	The release of β-acids from the film followed Fickian diffusion (n < 0.45).					
33887287	1	22	theme	supercritical	205:217	arg1	extract					227:233	supercritical CO2 hop extract	205:233	supercritical CO2 hop extract	205:233	β-Acids are natural antibacterial and antioxidant ingredients, obtained from supercritical CO2 hop extract.					
33887287	5	23	theme	-0.3	772:775	arg1	%					771:771	%	771:771	%	771:771	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	5	24	theme	upward	700:705	arg1	trend					707:711	a significant upward trend	686:711	a significant upward trend (1.9 MPa to 9.6 MPa)	686:732	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	5	24	theme	upward	700:705	arg1	MPa					729:731	1.9 MPa to 9.6 MPa	714:731	1.9 MPa to 9.6 MPa	714:731	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	1	25	theme	CO2	219:221	arg1	extract					227:233	supercritical CO2 hop extract	205:233	supercritical CO2 hop extract	205:233	β-Acids are natural antibacterial and antioxidant ingredients, obtained from supercritical CO2 hop extract.					
33887287	6	26	theme	excellent	827:835	arg1	capability					848:857	excellent UV barrier capability	827:857	excellent UV barrier capability	827:857	Interestingly, the chitosan-based films showed excellent UV barrier capability below 400 nm.					
33887287	1	27	theme	hop	223:225	arg1	extract					227:233	supercritical CO2 hop extract	205:233	supercritical CO2 hop extract	205:233	β-Acids are natural antibacterial and antioxidant ingredients, obtained from supercritical CO2 hop extract.					
33887287	5	28	theme	β-acids	751:757	arg1	%					776:776	0.1%-0.3%	768:776	0.1%-0.3%	768:776	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	5	28	theme	β-acids	751:757	arg1	content					759:765	β-acids content	751:765	β-acids content (0.1%-0.3%)	751:777	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	8	29	theme	pathogens	1020:1028	arg1	growth					994:999	the growth	990:999	the growth of five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes)	990:1140	In addition, the complex films inhibited the growth of five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes).					
33887287	9	30	theme	promising	1169:1177	arg1	nature					1179:1184	the promising nature	1165:1184	the promising nature of composite film	1165:1202	This study highlights the promising nature of composite film as a desirable alternative for active packaging.					
33887287	9	30	theme	promising	1169:1177	arg1	alternative					1219:1229	a desirable alternative	1207:1229	a desirable alternative for active packaging	1207:1250	This study highlights the promising nature of composite film as a desirable alternative for active packaging.					
33887287	7	31	dep	<	940:940	arg1	0.45					942:945	0.45	942:945	0.45	942:945	The release of β-acids from the film followed Fickian diffusion (n < 0.45).					
33887287	4	32	theme	Structure	514:522	arg1	analysis					524:531	Structure analysis	514:531	Structure analysis	514:531	Structure analysis revealed that β-acids can be successfully combined with the chitosan matrix.					
33887287	5	33	theme	1.9	714:716	arg1	MPa					718:720	MPa	718:720	MPa	718:720	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	3	34	dep	Fourier	428:434	arg1	transform					436:444	transform	436:444	transform infrared spectroscopy (FTIR)	436:473	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	8	35	theme	Listeria	1118:1125	arg1	monocytogenes					1127:1139	Listeria monocytogenes	1118:1139	Listeria monocytogenes	1118:1139	In addition, the complex films inhibited the growth of five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes).					
33887287	3	36	theme	Complex	322:328	arg1	films					330:334	Complex films	322:334	Complex films	322:334	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	6	37	theme	chitosan-based	799:812	arg1	films					814:818	the chitosan-based films	795:818	the chitosan-based films	795:818	Interestingly, the chitosan-based films showed excellent UV barrier capability below 400 nm.					
33887287	3	38	dep	transform	436:444	arg1	infrared					446:453	infrared	446:453	transform infrared spectroscopy (FTIR)	436:473	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	3	39	theme	X-ray	480:484	arg1	XRD					508:510	XRD	508:510	XRD	508:510	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	3	39	theme	X-ray	480:484	arg1	analysis					498:505	X-ray diffraction analysis	480:505	X-ray diffraction analysis (XRD)	480:511	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	5	40	theme	0.1	768:770	arg1	%					771:771	%	771:771	%	771:771	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	5	41	from	increase	739:746	arg1	%					776:776	0.1%-0.3%	768:776	0.1%-0.3%	768:776	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	5	41	from	increase	739:746	arg1	content					759:765	β-acids content	751:765	β-acids content (0.1%-0.3%)	751:777	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	3	42	theme	diffraction	486:496	arg1	XRD					508:510	XRD	508:510	XRD	508:510	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	3	42	theme	diffraction	486:496	arg1	analysis					498:505	X-ray diffraction analysis	480:505	X-ray diffraction analysis (XRD)	480:511	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	5	43	dep	MPa	718:720	arg1	to					722:723	to	722:723	to	722:723	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	9	44	theme	film	1199:1202	arg1	nature					1179:1184	the promising nature	1165:1184	the promising nature of composite film	1165:1202	This study highlights the promising nature of composite film as a desirable alternative for active packaging.					
33887287	9	44	theme	film	1199:1202	arg1	alternative					1219:1229	a desirable alternative	1207:1229	a desirable alternative for active packaging	1207:1250	This study highlights the promising nature of composite film as a desirable alternative for active packaging.					
33887287	5	45	theme	significant	688:698	arg1	trend					707:711	a significant upward trend	686:711	a significant upward trend (1.9 MPa to 9.6 MPa)	686:732	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	5	45	theme	significant	688:698	arg1	MPa					729:731	1.9 MPa to 9.6 MPa	714:731	1.9 MPa to 9.6 MPa	714:731	Mechanical tests demonstrated that the tensile strength of the films showed a significant upward trend (1.9 MPa to 9.6 MPa) with increase in β-acids content (0.1%-0.3%).					
33887287	2	46	theme	casting	306:312	arg1	method					314:319	the casting method	302:319	the casting method	302:319	In this study, β-acids/chitosan complex films were prepared using the casting method.					
33887287	1	47	theme	natural	140:146	arg1	β-Acids					128:134	β-Acids	128:134	β-Acids	128:134	β-Acids are natural antibacterial and antioxidant ingredients, obtained from supercritical CO2 hop extract.					
33887287	1	47	theme	natural	140:146	arg1	ingredients					178:188	natural antibacterial and antioxidant ingredients	140:188	natural antibacterial and antioxidant ingredients	140:188	β-Acids are natural antibacterial and antioxidant ingredients, obtained from supercritical CO2 hop extract.					
33887287	4	48	theme	chitosan	593:600	arg1	matrix					602:607	the chitosan matrix	589:607	the chitosan matrix	589:607	Structure analysis revealed that β-acids can be successfully combined with the chitosan matrix.					
33887287	0	49	theme	Structural	73:82	arg1	properties					116:125	Structural, physicochemical, and bioactive properties	73:125	Structural, physicochemical, and bioactive properties	73:125	Functional polysaccharide-based film prepared from chitosan and β-acids: Structural, physicochemical, and bioactive properties.					
33887287	2	50	theme	complex	268:274	arg1	films					276:280	β-acids/chitosan complex films	251:280	β-acids/chitosan complex films	251:280	In this study, β-acids/chitosan complex films were prepared using the casting method.					
33887287	7	51	theme	<	940:940	arg1	n					938:938	n	938:938	n < 0.45	938:945	The release of β-acids from the film followed Fickian diffusion (n < 0.45).					
33887287	1	52	theme	antibacterial	148:160	arg1	β-Acids					128:134	β-Acids	128:134	β-Acids	128:134	β-Acids are natural antibacterial and antioxidant ingredients, obtained from supercritical CO2 hop extract.					
33887287	1	52	theme	antibacterial	148:160	arg1	ingredients					178:188	natural antibacterial and antioxidant ingredients	140:188	natural antibacterial and antioxidant ingredients	140:188	β-Acids are natural antibacterial and antioxidant ingredients, obtained from supercritical CO2 hop extract.					
33887287	3	53	theme	scanning	361:368	arg1	microscopy					379:388	scanning electron microscopy	361:388	scanning electron microscopy (SEM)	361:394	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	3	53	theme	scanning	361:368	arg1	SEM					391:393	SEM	391:393	SEM	391:393	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	9	54	theme	desirable	1209:1217	arg1	nature					1179:1184	the promising nature	1165:1184	the promising nature of composite film	1165:1202	This study highlights the promising nature of composite film as a desirable alternative for active packaging.					
33887287	9	54	theme	desirable	1209:1217	arg1	alternative					1219:1229	a desirable alternative	1207:1229	a desirable alternative for active packaging	1207:1250	This study highlights the promising nature of composite film as a desirable alternative for active packaging.					
33887287	2	55	theme	β-acids/chitosan	251:266	arg1	films					276:280	β-acids/chitosan complex films	251:280	β-acids/chitosan complex films	251:280	In this study, β-acids/chitosan complex films were prepared using the casting method.					
33887287	3	56	theme	atomic	397:402	arg1	AFM					422:424	AFM	422:424	AFM	422:424	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	3	56	theme	atomic	397:402	arg1	microscope					410:419	atomic force microscope	397:419	atomic force microscope (AFM)	397:425	Complex films were characterized using scanning electron microscopy (SEM), atomic force microscope (AFM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD).					
33887287	8	57	theme	food-borne	1009:1018	arg1	pathogens					1020:1028	five food-borne pathogens	1004:1028	five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes)	1004:1140	In addition, the complex films inhibited the growth of five food-borne pathogens (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella enteritidis and Listeria monocytogenes).					
33806781	2	0	theme	aerated	377:383	arg1	drinks					417:422	milk shake type drinks	401:422	milk shake type drinks	401:422	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	2	0	theme	aerated	377:383	arg1	products					385:392	aerated products	377:392	aerated products (e.g., milk shake type drinks)	377:423	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	0	1	theme	Hydrolysate	108:118	arg1	Properties					33:42	Hypotensive and Hepatoprotective Properties	0:42	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.	0:136	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.					
33806781	0	2	theme	Proteins	128:135	arg1	Hydrolysate					108:118	the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate	47:118	the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins	47:135	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.					
33806781	6	3	theme	composition	926:936	arg1	properties					908:917	the hypotensive, antioxidant and hepatoprotective properties	858:917	the hypotensive, antioxidant and hepatoprotective properties of the composition	858:936	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	0	4	theme	Whey	123:126	arg1	Proteins					128:135	Whey Proteins	123:135	Whey Proteins	123:135	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.					
33806781	5	5	theme	Polysaccharide	674:687	arg1	content					689:695	Polysaccharide content	674:695	Polysaccharide content	674:695	Polysaccharide content was selected according to foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition.					
33806781	6	6	with	rats	1023:1026	arg1	injury					1054:1059	CCl4-induced hepatic injury	1033:1059	CCl4-induced hepatic injury	1033:1059	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	5	7	theme	angiotensin-I-converting	761:784	arg1	characteristics					804:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics	723:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition	723:846	Polysaccharide content was selected according to foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition.					
33806781	6	8	theme	in	953:954	arg1	tests					961:965	in vivo tests	953:965	in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury	953:1059	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	2	9	theme	shake	406:410	arg1	drinks					417:422	milk shake type drinks	401:422	milk shake type drinks	401:422	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	2	9	theme	shake	406:410	arg1	products					385:392	aerated products	377:392	aerated products (e.g., milk shake type drinks)	377:423	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	5	10	theme	enzyme	786:791	arg1	characteristics					804:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics	723:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition	723:846	Polysaccharide content was selected according to foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition.					
33806781	2	11	used	used	350:353	arg2	agents					366:371	foaming agents	358:371	foaming agents for aerated products (e.g., milk shake type drinks)	358:423	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	2	11	used	used	350:353	arg2	WPHs					331:334	WPHs	331:334	WPHs	331:334	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	4	12	theme	polysaccharides-pumpkin	582:604	arg1	pectin					606:611	three polysaccharides-pumpkin pectin	576:611	three polysaccharides-pumpkin pectin	576:611	Here, we present a composition including WPH and three polysaccharides-pumpkin pectin, sodium alginate and ι-carrageenan-used as foam stabilizers.					
33806781	2	13	theme	milk	401:404	arg1	drinks					417:422	milk shake type drinks	401:422	milk shake type drinks	401:422	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	2	13	theme	milk	401:404	arg1	products					385:392	aerated products	377:392	aerated products (e.g., milk shake type drinks)	377:423	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	5	14	dep	foaming	723:729	arg1	organoleptic					732:743	organoleptic	732:743	organoleptic	732:743	Polysaccharide content was selected according to foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition.					
33806781	5	15	theme	inhibitory	793:802	arg1	characteristics					804:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics	723:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition	723:846	Polysaccharide content was selected according to foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition.					
33806781	6	16	with	rats	1007:1010	arg1	injury					1054:1059	CCl4-induced hepatic injury	1033:1059	CCl4-induced hepatic injury	1033:1059	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	1	17	theme	promising	191:199	arg1	sources					201:207	the most promising sources	182:207	the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others	182:328	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	0	18	theme	Hypotensive	0:10	arg1	Properties					33:42	Hypotensive and Hepatoprotective Properties	0:42	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.	0:136	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.					
33806781	6	19	theme	hepatic	1046:1052	arg1	injury					1054:1059	CCl4-induced hepatic injury	1033:1059	CCl4-induced hepatic injury	1033:1059	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	1	20	theme	sources	201:207	arg1	sources					201:207	the most promising sources	182:207	the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others	182:328	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	1	20	theme	sources	201:207	arg1	one					175:177	one	175:177	one	175:177	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	6	21	theme	CCl4-induced	1033:1044	arg1	injury					1054:1059	CCl4-induced hepatic injury	1033:1059	CCl4-induced hepatic injury	1033:1059	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	1	22	with	peptides	226:233	arg1	properties					256:265	such beneficial properties	240:265	such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others	240:328	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	3	23	theme	bitter	451:456	arg1	taste					458:462	a bitter taste	449:462	a bitter taste	449:462	However, WPH alone has a bitter taste and foamed WPH should be stabilized by additional ingredients.					
33806781	0	24	theme	Hepatoprotective	16:31	arg1	Properties					33:42	Hypotensive and Hepatoprotective Properties	0:42	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.	0:136	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.					
33806781	5	25	theme	foaming	723:729	arg1	characteristics					804:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics	723:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition	723:846	Polysaccharide content was selected according to foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition.					
33806781	1	26	theme	Whey	138:141	arg1	WPHs					165:168	WPHs	165:168	WPHs	165:168	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	1	26	theme	Whey	138:141	arg1	hydrolysates					151:162	Whey protein hydrolysates	138:162	Whey protein hydrolysates (WPHs)	138:169	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	1	27	theme	biofunctional	212:224	arg1	peptides					226:233	biofunctional peptides	212:233	biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others	212:328	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	5	28	theme	resulted	827:834	arg1	composition					836:846	the resulted composition	823:846	the resulted composition	823:846	Polysaccharide content was selected according to foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition.					
33806781	3	29	contain	has	445:447	arg1	WPH					435:437	WPH	435:437	WPH	435:437	However, WPH alone has a bitter taste and foamed WPH should be stabilized by additional ingredients.					
33806781	3	29	contain	has	445:447	arg2	taste					458:462	a bitter taste	449:462	a bitter taste	449:462	However, WPH alone has a bitter taste and foamed WPH should be stabilized by additional ingredients.					
33806781	2	30	dep	drinks	417:422	arg1	e.g.					395:398	e.g.	395:398	e.g.	395:398	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	1	31	theme	peptides	226:233	arg1	sources					201:207	the most promising sources	182:207	the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others	182:328	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	5	32	theme	composition	836:846	arg1	characteristics					804:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics	723:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition	723:846	Polysaccharide content was selected according to foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition.					
33806781	6	33	theme	Wistar	1016:1021	arg1	rats					1023:1026	Wistar rats	1016:1026	Wistar rats	1016:1026	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	2	34	theme	type	412:415	arg1	drinks					417:422	milk shake type drinks	401:422	milk shake type drinks	401:422	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	2	34	theme	type	412:415	arg1	products					385:392	aerated products	377:392	aerated products (e.g., milk shake type drinks)	377:423	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	0	35	theme	Foaming	77:83	arg1	Hydrolysate					108:118	the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate	47:118	the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins	47:135	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.					
33806781	5	36	theme	antioxidant	745:755	arg1	characteristics					804:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics	723:818	foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition	723:846	Polysaccharide content was selected according to foaming, organoleptic antioxidant and angiotensin-I-converting enzyme inhibitory characteristics of the resulted composition.					
33806781	1	37	theme	protein	143:149	arg1	WPHs					165:168	WPHs	165:168	WPHs	165:168	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	1	37	theme	protein	143:149	arg1	hydrolysates					151:162	Whey protein hydrolysates	138:162	Whey protein hydrolysates (WPHs)	138:169	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	0	38	theme	Polysaccharide-Stabilized	51:75	arg1	Hydrolysate					108:118	the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate	47:118	the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins	47:135	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.					
33806781	2	39	theme	foaming	358:364	arg1	agents					366:371	foaming agents	358:371	foaming agents for aerated products (e.g., milk shake type drinks)	358:423	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	2	39	theme	foaming	358:364	arg1	WPHs					331:334	WPHs	331:334	WPHs	331:334	WPHs also could be used as foaming agents for aerated products (e.g., milk shake type drinks).					
33806781	6	40	theme	hypertensive	994:1005	arg1	rats					1007:1010	spontaneously hypertensive rats	980:1010	spontaneously hypertensive rats	980:1010	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	6	41	theme	hepatoprotective	891:906	arg1	properties					908:917	the hypotensive, antioxidant and hepatoprotective properties	858:917	the hypotensive, antioxidant and hepatoprotective properties of the composition	858:936	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	6	42	theme	hypotensive	862:872	arg1	properties					908:917	the hypotensive, antioxidant and hepatoprotective properties	858:917	the hypotensive, antioxidant and hepatoprotective properties of the composition	858:936	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	1	43	theme	such	240:243	arg1	properties					256:265	such beneficial properties	240:265	such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others	240:328	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	3	44	theme	foamed	468:473	arg1	WPH					475:477	foamed WPH	468:477	foamed WPH	468:477	However, WPH alone has a bitter taste and foamed WPH should be stabilized by additional ingredients.					
33806781	1	45	theme	beneficial	245:254	arg1	properties					256:265	such beneficial properties	240:265	such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others	240:328	Whey protein hydrolysates (WPHs) are one of the most promising sources of biofunctional peptides with such beneficial properties as antioxidant, antihypertensive, anti-inflammatory and others.					
33806781	0	46	theme	Containing	97:106	arg1	Hydrolysate					108:118	the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate	47:118	the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins	47:135	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.					
33806781	6	47	theme	antioxidant	875:885	arg1	properties					908:917	the hypotensive, antioxidant and hepatoprotective properties	858:917	the hypotensive, antioxidant and hepatoprotective properties of the composition	858:936	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33806781	4	48	theme	foam	656:659	arg1	stabilizers					661:671	foam stabilizers	656:671	foam stabilizers	656:671	Here, we present a composition including WPH and three polysaccharides-pumpkin pectin, sodium alginate and ι-carrageenan-used as foam stabilizers.					
33806781	4	49	theme	sodium	614:619	arg1	alginate					621:628	sodium alginate	614:628	sodium alginate	614:628	Here, we present a composition including WPH and three polysaccharides-pumpkin pectin, sodium alginate and ι-carrageenan-used as foam stabilizers.					
33806781	0	50	theme	Composition	85:95	arg1	Hydrolysate					108:118	the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate	47:118	the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins	47:135	Hypotensive and Hepatoprotective Properties of the Polysaccharide-Stabilized Foaming Composition Containing Hydrolysate of Whey Proteins.					
33806781	3	51	theme	additional	503:512	arg1	ingredients					514:524	additional ingredients	503:524	additional ingredients	503:524	However, WPH alone has a bitter taste and foamed WPH should be stabilized by additional ingredients.					
33806781	6	52	dep	in	953:954	arg1	vivo					956:959	vivo	956:959	vivo	956:959	Further, the hypotensive, antioxidant and hepatoprotective properties of the composition were proved by in vivo tests performed in spontaneously hypertensive rats and Wistar rats with CCl4-induced hepatic injury.					
33387794	6	0	theme	vesicle	942:948	arg1	size					950:953	The vesicle size	938:953	The vesicle size	938:953	The vesicle size increased with the LTA molar concentration up to ∼6.5 mol%, accompanied by a broadened size distribution, and further increasing the LTA concentration led to a decrease in the vesicle size.					
33387794	4	1	theme	bacteria	631:638	arg1	membranes					640:648	Gram-positive bacteria membranes	617:648	Gram-positive bacteria membranes	617:648	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	4	2	from	varied	707:712	arg1	changes					555:561	structural changes	544:561	structural changes	544:561	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	1	3	theme	structural	255:264	arg1	component					290:298	a key structural and functional membrane component	249:298	a key structural and functional membrane component	249:298	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	1	3	theme	structural	255:264	arg1	acid					123:126	Lipoteichoic acid	110:126	Lipoteichoic acid (LTA)	110:132	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	4	4	theme	LTA	699:701	arg1	concentration					664:676	the concentration	660:676	the concentration of Bacillus Subtilis LTA	660:701	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	4	4	theme	LTA	699:701	arg1	varied					707:712	varied	707:712	varied	707:712	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	8	5	with	formation	1283:1291	arg1	addition					1324:1331	LTA addition	1320:1331	LTA addition	1320:1331	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	0	6	contain	containing	16:25	arg1	liposomes					6:14	Mixed liposomes	0:14	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA)	0:80	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA) induced structural changes.					
33387794	0	6	contain	containing	16:25	arg2	bacteria					41:48	gram-positive bacteria	27:48	gram-positive bacteria lipids: Lipoteichoic acid (LTA)	27:80	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA) induced structural changes.					
33387794	1	7	theme	polymer	156:162	arg1	amphiphile					164:173	a surface associated polymer amphiphile	135:173	a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane	135:243	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	1	7	theme	polymer	156:162	arg1	acid					123:126	Lipoteichoic acid	110:126	Lipoteichoic acid (LTA)	110:132	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	9	8	theme	LTA	1683:1685	arg1	composition					1687:1697	the LTA composition	1679:1697	the LTA composition	1679:1697	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	5	9	theme	charged	910:916	arg1	polyphosphates					922:935	the negatively charged LTA polyphosphates	895:935	the negatively charged LTA polyphosphates	895:935	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	5	10	theme	Small-angle	733:743	arg1	SANS					765:768	SANS	765:768	SANS	765:768	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	5	10	theme	Small-angle	733:743	arg1	scattering					753:762	Small-angle neutron scattering	733:762	Small-angle neutron scattering (SANS)	733:769	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	4	11	theme	Bacillus	681:688	arg1	LTA					699:701	Bacillus Subtilis LTA	681:701	Bacillus Subtilis LTA	681:701	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	5	12	theme	scattering	753:762	arg1	measurements					806:817	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements	733:817	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements	733:817	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	5	13	theme	mixed	842:846	arg1	vesicles					860:867	mixed unilamellar vesicles	842:867	mixed unilamellar vesicles	842:867	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	1	14	theme	Gram-positive	200:212	arg1	membrane					236:243	the Gram-positive bacterial cytoplasmic membrane	196:243	the Gram-positive bacterial cytoplasmic membrane	196:243	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	8	15	theme	Complementary	1230:1242	arg1	imaging					1253:1259	Complementary Cryo-TEM imaging	1230:1259	Complementary Cryo-TEM imaging	1230:1259	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	6	16	theme	molar	978:982	arg1	concentration					984:996	the LTA molar concentration	970:996	the LTA molar concentration	970:996	The vesicle size increased with the LTA molar concentration up to ∼6.5 mol%, accompanied by a broadened size distribution, and further increasing the LTA concentration led to a decrease in the vesicle size.					
33387794	7	17	with	vesicles	1200:1207	arg1	shells					1222:1227	thinner shells	1214:1227	thinner shells	1214:1227	At 80 °C, SANS analyses showed the formation of larger vesicles with thinner shells.					
33387794	9	18	theme	electrostatic	1496:1508	arg1	interactions					1510:1521	the steric and electrostatic interactions	1481:1521	the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane	1481:1714	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	8	19	theme	LTA	1320:1322	arg1	addition					1324:1331	LTA addition	1320:1331	LTA addition	1320:1331	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	6	20	theme	size	1042:1045	arg1	distribution					1047:1058	a broadened size distribution	1030:1058	a broadened size distribution	1030:1058	The vesicle size increased with the LTA molar concentration up to ∼6.5 mol%, accompanied by a broadened size distribution, and further increasing the LTA concentration led to a decrease in the vesicle size.					
33387794	4	21	theme	0-15	722:725	arg1	mol					727:729	mol	727:729	mol	727:729	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	5	22	theme	light	783:787	arg1	DLS					801:803	DLS	801:803	DLS	801:803	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	5	22	theme	light	783:787	arg1	scattering					789:798	dynamic light scattering	775:798	dynamic light scattering (DLS)	775:804	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	9	23	theme	bulky	1530:1534	arg1	molecules					1540:1548	the bulky LTA molecules	1526:1548	the bulky LTA molecules	1526:1548	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	1	24	theme	surface	137:143	arg1	amphiphile					164:173	a surface associated polymer amphiphile	135:173	a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane	135:243	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	1	24	theme	surface	137:143	arg1	acid					123:126	Lipoteichoic acid	110:126	Lipoteichoic acid (LTA)	110:132	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	9	25	theme	increased	1555:1563	arg1	fluidity					1571:1578	increased chain fluidity	1555:1578	increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane	1555:1714	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	9	26	with	interactions	1510:1521	arg1	fluidity					1571:1578	increased chain fluidity	1555:1578	increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane	1555:1714	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	2	27	dep	different	364:372	arg1	physiological					374:386	physiological	374:386	physiological	374:386	Its composition in the membrane is regulated by bacteria under different physiological conditions.					
33387794	3	28	theme	membrane	451:458	arg1	stability					471:479	the membrane structural stability	447:479	the membrane structural stability	447:479	How such LTA compositional variations modulate the membrane structural stability and integrity is poorly understood.					
33387794	7	29	theme	larger	1193:1198	arg1	vesicles					1200:1207	larger vesicles	1193:1207	larger vesicles with thinner shells	1193:1227	At 80 °C, SANS analyses showed the formation of larger vesicles with thinner shells.					
33387794	8	30	theme	spherical	1376:1384	arg1	aggregates					1386:1395	interconnected spherical aggregates	1361:1395	interconnected spherical aggregates	1361:1395	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	0	31	theme	Mixed	0:4	arg1	liposomes					6:14	Mixed liposomes	0:14	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA)	0:80	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA) induced structural changes.					
33387794	5	32	theme	unilamellar	848:858	arg1	vesicles					860:867	mixed unilamellar vesicles	842:867	mixed unilamellar vesicles	842:867	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	1	33	theme	membrane	281:288	arg1	component					290:298	a key structural and functional membrane component	249:298	a key structural and functional membrane component	249:298	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	1	33	theme	membrane	281:288	arg1	acid					123:126	Lipoteichoic acid	110:126	Lipoteichoic acid (LTA)	110:132	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	3	34	theme	compositional	413:425	arg1	variations					427:436	such LTA compositional variations	404:436	such LTA compositional variations	404:436	How such LTA compositional variations modulate the membrane structural stability and integrity is poorly understood.					
33387794	8	35	theme	size	1408:1411	arg1	presence					1349:1356	the presence	1345:1356	the presence of interconnected spherical aggregates of smaller size	1345:1411	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	8	35	theme	size	1408:1411	arg1	formation					1283:1291	vesicle formation	1275:1291	vesicle formation	1275:1291	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	8	35	theme	size	1408:1411	arg1	increase					1306:1313	the size increase	1297:1313	the size increase	1297:1313	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	7	36	theme	SANS	1155:1158	arg1	analyses					1160:1167	SANS analyses	1155:1167	SANS analyses	1155:1167	At 80 °C, SANS analyses showed the formation of larger vesicles with thinner shells.					
33387794	3	37	theme	such	404:407	arg1	variations					427:436	such LTA compositional variations	404:436	such LTA compositional variations	404:436	How such LTA compositional variations modulate the membrane structural stability and integrity is poorly understood.					
33387794	4	38	theme	structural	544:553	arg1	changes					555:561	structural changes	544:561	structural changes	544:561	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	8	39	theme	higher	1416:1421	arg1	concentrations					1427:1440	higher LTA concentrations	1416:1440	higher LTA concentrations	1416:1440	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	0	40	dep	bacteria	41:48	arg1	LTA					77:79	LTA	77:79	LTA	77:79	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA) induced structural changes.					
33387794	0	40	dep	bacteria	41:48	arg1	lipids					50:55	lipids	50:55	gram-positive bacteria lipids: Lipoteichoic acid (LTA)	27:80	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA) induced structural changes.					
33387794	0	40	dep	bacteria	41:48	arg1	acid					71:74	Lipoteichoic acid	58:74	gram-positive bacteria lipids: Lipoteichoic acid (LTA)	27:80	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA) induced structural changes.					
33387794	1	41	theme	bacterial	214:222	arg1	membrane					236:243	the Gram-positive bacterial cytoplasmic membrane	196:243	the Gram-positive bacterial cytoplasmic membrane	196:243	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	4	42	theme	membranes	640:648	arg1	composition					602:612	the lipid composition	592:612	the lipid composition of Gram-positive bacteria membranes	592:648	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	2	43	theme	different	364:372	arg1	conditions					388:397	different physiological conditions	364:397	different physiological conditions	364:397	Its composition in the membrane is regulated by bacteria under different physiological conditions.					
33387794	0	44	theme	Lipoteichoic	58:69	arg1	LTA					77:79	LTA	77:79	LTA	77:79	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA) induced structural changes.					
33387794	0	44	theme	Lipoteichoic	58:69	arg1	acid					71:74	Lipoteichoic acid	58:74	gram-positive bacteria lipids: Lipoteichoic acid (LTA)	27:80	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA) induced structural changes.					
33387794	7	45	theme	thinner	1214:1220	arg1	shells					1222:1227	thinner shells	1214:1227	thinner shells	1214:1227	At 80 °C, SANS analyses showed the formation of larger vesicles with thinner shells.					
33387794	6	46	from	decrease	1115:1122	arg1	size					1139:1142	the vesicle size	1127:1142	the vesicle size	1127:1142	The vesicle size increased with the LTA molar concentration up to ∼6.5 mol%, accompanied by a broadened size distribution, and further increasing the LTA concentration led to a decrease in the vesicle size.					
33387794	1	47	theme	key	251:253	arg1	component					290:298	a key structural and functional membrane component	249:298	a key structural and functional membrane component	249:298	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	1	47	theme	key	251:253	arg1	acid					123:126	Lipoteichoic acid	110:126	Lipoteichoic acid (LTA)	110:132	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	4	48	theme	Gram-positive	617:629	arg1	membranes					640:648	Gram-positive bacteria membranes	617:648	Gram-positive bacteria membranes	617:648	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	1	49	theme	associated	145:154	arg1	amphiphile					164:173	a surface associated polymer amphiphile	135:173	a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane	135:243	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	1	49	theme	associated	145:154	arg1	acid					123:126	Lipoteichoic acid	110:126	Lipoteichoic acid (LTA)	110:132	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	8	50	theme	Cryo-TEM	1244:1251	arg1	imaging					1253:1259	Complementary Cryo-TEM imaging	1230:1259	Complementary Cryo-TEM imaging	1230:1259	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	6	51	theme	mol	1009:1011	arg1	%					1012:1012	∼6.5 mol%	1004:1012	∼6.5 mol%	1004:1012	The vesicle size increased with the LTA molar concentration up to ∼6.5 mol%, accompanied by a broadened size distribution, and further increasing the LTA concentration led to a decrease in the vesicle size.					
33387794	5	52	theme	vesicles	860:867	arg1	formation					829:837	formation	829:837	formation of mixed unilamellar vesicles	829:867	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	9	53	theme	molecules	1540:1548	arg1	interactions					1510:1521	the steric and electrostatic interactions	1481:1521	the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane	1481:1714	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	8	54	theme	vesicle	1275:1281	arg1	formation					1283:1291	vesicle formation	1275:1291	vesicle formation	1275:1291	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	5	55	theme	neutron	745:751	arg1	SANS					765:768	SANS	765:768	SANS	765:768	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	5	55	theme	neutron	745:751	arg1	scattering					753:762	Small-angle neutron scattering	733:762	Small-angle neutron scattering (SANS)	733:769	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	7	56	theme	vesicles	1200:1207	arg1	formation					1180:1188	the formation	1176:1188	the formation of larger vesicles with thinner shells	1176:1227	At 80 °C, SANS analyses showed the formation of larger vesicles with thinner shells.					
33387794	1	57	theme	Lipoteichoic	110:121	arg1	amphiphile					164:173	a surface associated polymer amphiphile	135:173	a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane	135:243	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	1	57	theme	Lipoteichoic	110:121	arg1	component					290:298	a key structural and functional membrane component	249:298	a key structural and functional membrane component	249:298	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	1	57	theme	Lipoteichoic	110:121	arg1	LTA					129:131	LTA	129:131	LTA	129:131	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	1	57	theme	Lipoteichoic	110:121	arg1	acid					123:126	Lipoteichoic acid	110:126	Lipoteichoic acid (LTA)	110:132	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	6	58	theme	LTA	974:976	arg1	concentration					984:996	the LTA molar concentration	970:996	the LTA molar concentration	970:996	The vesicle size increased with the LTA molar concentration up to ∼6.5 mol%, accompanied by a broadened size distribution, and further increasing the LTA concentration led to a decrease in the vesicle size.					
33387794	8	59	theme	size	1301:1304	arg1	increase					1306:1313	the size increase	1297:1313	the size increase	1297:1313	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	9	60	theme	interactions	1510:1521	arg1	light					1472:1476	light	1472:1476	light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane	1472:1714	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	9	61	from	temperature	1594:1604	arg1	fluidity					1571:1578	increased chain fluidity	1555:1578	increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane	1555:1714	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	8	62	with	presence	1349:1356	arg1	addition					1324:1331	LTA addition	1320:1331	LTA addition	1320:1331	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	5	63	theme	dynamic	775:781	arg1	DLS					801:803	DLS	801:803	DLS	801:803	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	5	63	theme	dynamic	775:781	arg1	scattering					789:798	dynamic light scattering	775:798	dynamic light scattering (DLS)	775:804	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	5	64	theme	LTA	918:920	arg1	polyphosphates					922:935	the negatively charged LTA polyphosphates	895:935	the negatively charged LTA polyphosphates	895:935	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	6	65	theme	broadened	1032:1040	arg1	distribution					1047:1058	a broadened size distribution	1030:1058	a broadened size distribution	1030:1058	The vesicle size increased with the LTA molar concentration up to ∼6.5 mol%, accompanied by a broadened size distribution, and further increasing the LTA concentration led to a decrease in the vesicle size.					
33387794	5	66	theme	scattering	789:798	arg1	measurements					806:817	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements	733:817	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements	733:817	Small-angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements indicated formation of mixed unilamellar vesicles, presumably stabilized by the negatively charged LTA polyphosphates.					
33387794	9	67	theme	LTA	1536:1538	arg1	molecules					1540:1548	the bulky LTA molecules	1526:1548	the bulky LTA molecules	1526:1548	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	9	68	from	fluidity	1571:1578	arg1	membrane					1707:1714	the membrane	1703:1714	the membrane	1703:1714	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	4	69	theme	mol	727:729	arg1	%					730:730	0-15 mol%	722:730	0-15 mol%	722:730	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	0	70	theme	structural	90:99	arg1	changes					101:107	structural changes	90:107	structural changes	90:107	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA) induced structural changes.					
33387794	2	71	from	composition	305:315	arg1	membrane					324:331	the membrane	320:331	the membrane	320:331	Its composition in the membrane is regulated by bacteria under different physiological conditions.					
33387794	8	72	dep	formation	1283:1291	arg1	the					1271:1273	the	1271:1273	the	1271:1273	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	3	73	theme	structural	460:469	arg1	stability					471:479	the membrane structural stability	447:479	the membrane structural stability	447:479	How such LTA compositional variations modulate the membrane structural stability and integrity is poorly understood.					
33387794	6	74	theme	LTA	1088:1090	arg1	concentration					1092:1104	the LTA concentration	1084:1104	the LTA concentration led to a decrease in the vesicle size	1084:1142	The vesicle size increased with the LTA molar concentration up to ∼6.5 mol%, accompanied by a broadened size distribution, and further increasing the LTA concentration led to a decrease in the vesicle size.					
33387794	8	75	theme	interconnected	1361:1374	arg1	aggregates					1386:1395	interconnected spherical aggregates	1361:1395	interconnected spherical aggregates	1361:1395	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	0	76	theme	gram-positive	27:39	arg1	bacteria					41:48	gram-positive bacteria	27:48	gram-positive bacteria lipids: Lipoteichoic acid (LTA)	27:80	Mixed liposomes containing gram-positive bacteria lipids: Lipoteichoic acid (LTA) induced structural changes.					
33387794	1	77	theme	functional	270:279	arg1	component					290:298	a key structural and functional membrane component	249:298	a key structural and functional membrane component	249:298	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	1	77	theme	functional	270:279	arg1	acid					123:126	Lipoteichoic acid	110:126	Lipoteichoic acid (LTA)	110:132	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	9	78	theme	higher	1587:1592	arg1	temperature					1594:1604	the higher temperature	1583:1604	the higher temperature	1583:1604	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	4	79	theme	lipid	596:600	arg1	composition					602:612	the lipid composition	592:612	the lipid composition of Gram-positive bacteria membranes	592:648	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	9	80	theme	chain	1565:1569	arg1	fluidity					1571:1578	increased chain fluidity	1555:1578	increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane	1555:1714	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	8	81	theme	aggregates	1386:1395	arg1	presence					1349:1356	the presence	1345:1356	the presence of interconnected spherical aggregates of smaller size	1345:1411	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	8	81	theme	aggregates	1386:1395	arg1	formation					1283:1291	vesicle formation	1275:1291	vesicle formation	1275:1291	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	8	81	theme	aggregates	1386:1395	arg1	increase					1306:1313	the size increase	1297:1313	the size increase	1297:1313	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	4	82	dep	Bacillus	681:688	arg1	Subtilis					690:697	Subtilis	690:697	Subtilis	690:697	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	8	83	with	increase	1306:1313	arg1	addition					1324:1331	LTA addition	1320:1331	LTA addition	1320:1331	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	8	84	theme	smaller	1400:1406	arg1	size					1408:1411	smaller size	1400:1411	smaller size	1400:1411	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	4	85	from	changes	555:561	arg1	concentration					664:676	the concentration	660:676	the concentration of Bacillus Subtilis LTA	660:701	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	4	85	from	changes	555:561	arg1	liposomes					572:580	mixed liposomes	566:580	mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes	566:648	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	4	85	from	changes	555:561	arg1	varied					707:712	varied	707:712	varied	707:712	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	3	86	theme	LTA	409:411	arg1	variations					427:436	such LTA compositional variations	404:436	such LTA compositional variations	404:436	How such LTA compositional variations modulate the membrane structural stability and integrity is poorly understood.					
33387794	4	87	theme	mixed	566:570	arg1	liposomes					572:580	mixed liposomes	566:580	mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes	566:648	Here, we have investigated structural changes in mixed liposomes mimicking the lipid composition of Gram-positive bacteria membranes, in which the concentration of Bacillus Subtilis LTA was varied between 0-15 mol%.					
33387794	9	88	theme	molecular	1624:1632	arg1	packing					1634:1640	the molecular packing	1620:1640	the molecular packing	1620:1640	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
33387794	6	89	theme	vesicle	1131:1137	arg1	size					1139:1142	the vesicle size	1127:1142	the vesicle size	1127:1142	The vesicle size increased with the LTA molar concentration up to ∼6.5 mol%, accompanied by a broadened size distribution, and further increasing the LTA concentration led to a decrease in the vesicle size.					
33387794	8	90	theme	LTA	1423:1425	arg1	concentrations					1427:1440	higher LTA concentrations	1416:1440	higher LTA concentrations	1416:1440	Complementary Cryo-TEM imaging confirmed the vesicle formation and the size increase with LTA addition, as well as the presence of interconnected spherical aggregates of smaller size at higher LTA concentrations.					
33387794	1	91	theme	cytoplasmic	224:234	arg1	membrane					236:243	the Gram-positive bacterial cytoplasmic membrane	196:243	the Gram-positive bacterial cytoplasmic membrane	196:243	Lipoteichoic acid (LTA), a surface associated polymer amphiphile tethered directly to the Gram-positive bacterial cytoplasmic membrane, is a key structural and functional membrane component.					
33387794	9	92	theme	steric	1485:1490	arg1	interactions					1510:1521	the steric and electrostatic interactions	1481:1521	the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane	1481:1714	The results are discussed in light of the steric and electrostatic interactions of the bulky LTA molecules with increased chain fluidity at the higher temperature, which affect the molecular packing and interactions, and thus depend on the LTA composition, in the membrane.					
34757822	12	0	from	infants	2123:2129	arg1	Isolates					2099:2106	Isolates	2099:2106	Isolates from breastfed infants	2099:2129	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	9	1	theme	distinct	1667:1674	arg1	pattern					1692:1698	each strain's distinct HMO consumption pattern	1653:1698	each strain's distinct HMO consumption pattern	1653:1698	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	4	2	theme	pseudocatenulatum	590:606	arg1	difference					671:680	a difference	669:680	a difference driven by variable catabolism of fucosylated HMOs	669:730	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	2	theme	pseudocatenulatum	590:606	arg1	isolates					609:616	isolates	609:616	isolates that readily consumed HMOs	609:643	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	2	theme	pseudocatenulatum	590:606	arg1	groups					577:582	two distinct groups	564:582	two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not	564:666	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	2	theme	pseudocatenulatum	590:606	arg1	pseudocatenulatum					590:606	B. pseudocatenulatum	587:606	B. pseudocatenulatum	587:606	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	2	theme	pseudocatenulatum	590:606	arg1	those					649:653	those	649:653	those	649:653	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	7	3	theme	linkages	1147:1154	arg1	repertoire					1133:1142	the complete repertoire	1120:1142	the complete repertoire of linkages within the fucosylated HMO pool	1120:1186	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	7	3	theme	linkages	1147:1154	arg1	linkages					1147:1154	linkages	1147:1154	linkages within the fucosylated HMO pool	1147:1186	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	9	4	theme	family	1452:1457	arg1	protein					1476:1482	the family 1 solute-binding protein	1448:1482	the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80	1448:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	9	5	theme	consumption	1680:1690	arg1	pattern					1692:1698	each strain's distinct HMO consumption pattern	1653:1698	each strain's distinct HMO consumption pattern	1653:1698	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	4	6	theme	isolates	609:616	arg1	difference					671:680	a difference	669:680	a difference driven by variable catabolism of fucosylated HMOs	669:730	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	6	theme	isolates	609:616	arg1	isolates					609:616	isolates	609:616	isolates that readily consumed HMOs	609:643	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	6	theme	isolates	609:616	arg1	groups					577:582	two distinct groups	564:582	two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not	564:666	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	6	theme	isolates	609:616	arg1	pseudocatenulatum					590:606	B. pseudocatenulatum	587:606	B. pseudocatenulatum	587:606	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	6	theme	isolates	609:616	arg1	those					649:653	those	649:653	those	649:653	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	2	7	theme	gastrointestinal	298:313	arg1	tracts					315:320	the gastrointestinal tracts	294:320	the gastrointestinal tracts of adults and breastfed infants	294:352	One species, Bifidobacterium pseudocatenulatum, is found in the gastrointestinal tracts of adults and breastfed infants.					
34757822	9	8	theme	solute-binding	1461:1474	arg1	protein					1476:1482	the family 1 solute-binding protein	1448:1482	the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80	1448:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	8	9	from	activities	1239:1248	arg1	HMOs					1262:1265	HMOs	1262:1265	HMOs	1262:1265	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	8	10	with	specificities	1297:1309	arg1	enzyme					1325:1330	the GH95 enzyme	1316:1330	the GH95 enzyme preferring 1-2 fucosyl linkages	1316:1362	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	8	10	with	specificities	1297:1309	arg1	enzyme					1377:1382	the GH29 enzyme	1368:1382	the GH29 enzyme favoring 1-3 and 1-4 linkages	1368:1412	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	0	11	theme	Fucosylated	0:10	arg1	Foraging					39:46	Fucosylated Human Milk Oligosaccharide Foraging	0:46	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum	0:99	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity.					
34757822	1	12	theme	genus	193:197	arg1	Bifidobacterium					199:213	the genus Bifidobacterium	189:213	the genus Bifidobacterium	189:213	Human milk enriches members of the genus Bifidobacterium in the infant gut.					
34757822	8	13	theme	purified	1205:1212	arg1	GH29					1214:1217	the purified GH29	1201:1217	the purified GH29	1201:1217	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	8	14	theme	complementing	1276:1288	arg1	specificities					1297:1309	complementing enzyme specificities	1276:1309	complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages	1276:1412	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	4	15	from	growth	499:504	arg1	oligosaccharides					527:542	pooled human milk oligosaccharides	509:542	pooled human milk oligosaccharides (HMOs)	509:549	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	15	from	growth	499:504	arg1	HMOs					545:548	HMOs	545:548	HMOs	545:548	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	6	16	gly	fucosylated	975:985	arg1	HMOs					987:990	fucosylated HMOs	975:990	fucosylated HMOs tested	975:997	One isolate, B. pseudocatenulatum MP80, which uniquely possessed GH95 and GH29 α-fucosidases, consumed the majority of fucosylated HMOs tested.					
34757822	10	17	theme	HMO	1827:1829	arg1	pattern					1843:1849	the strain-specific fucosylated HMO utilization pattern	1795:1849	the strain-specific fucosylated HMO utilization pattern	1795:1849	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	16	18	theme	bacterial	2706:2714	arg1	colonization					2716:2727	bacterial colonization	2706:2727	bacterial colonization of the infant gut	2706:2745	Such knowledge furthers our understanding of how diet drives bacterial colonization of the infant gut.					
34757822	11	19	theme	bacterial	2005:2013	arg1	Bifidobacteriaceae					2022:2039	the bacterial family Bifidobacteriaceae	2001:2039	the bacterial family Bifidobacteriaceae	2001:2039	IMPORTANCE Often isolated from the human gut, microbes from the bacterial family Bifidobacteriaceae commonly possess genes enabling carbohydrate utilization.					
34757822	4	20	theme	those	649:653	arg1	difference					671:680	a difference	669:680	a difference driven by variable catabolism of fucosylated HMOs	669:730	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	20	theme	those	649:653	arg1	isolates					609:616	isolates	609:616	isolates that readily consumed HMOs	609:643	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	20	theme	those	649:653	arg1	groups					577:582	two distinct groups	564:582	two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not	564:666	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	20	theme	those	649:653	arg1	pseudocatenulatum					590:606	B. pseudocatenulatum	587:606	B. pseudocatenulatum	587:606	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	20	theme	those	649:653	arg1	those					649:653	those	649:653	those	649:653	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	21	gly	fucosylated	715:725	arg1	HMOs					727:730	fucosylated HMOs	715:730	fucosylated HMOs	715:730	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	10	22	theme	strain-specific	1799:1813	arg1	pattern					1843:1849	the strain-specific fucosylated HMO utilization pattern	1795:1849	the strain-specific fucosylated HMO utilization pattern	1795:1849	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	1	23	theme	infant	222:227	arg1	gut					229:231	the infant gut	218:231	the infant gut	218:231	Human milk enriches members of the genus Bifidobacterium in the infant gut.					
34757822	8	24	theme	fucosidase	1228:1237	arg1	activities					1239:1248	GH95 fucosidase activities	1223:1248	GH95 fucosidase activities directly on HMOs	1223:1265	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	6	25	theme	fucosylated	975:985	arg1	HMOs					987:990	fucosylated HMOs	975:990	fucosylated HMOs tested	975:997	One isolate, B. pseudocatenulatum MP80, which uniquely possessed GH95 and GH29 α-fucosidases, consumed the majority of fucosylated HMOs tested.					
34757822	11	26	from	Bifidobacteriaceae	2022:2039	arg1	microbes					1987:1994	microbes	1987:1994	microbes from the bacterial family Bifidobacteriaceae	1987:2039	IMPORTANCE Often isolated from the human gut, microbes from the bacterial family Bifidobacteriaceae commonly possess genes enabling carbohydrate utilization.					
34757822	6	27	contain	possessed	911:919	arg1	isolate					860:866	One isolate	856:866	One isolate	856:866	One isolate, B. pseudocatenulatum MP80, which uniquely possessed GH95 and GH29 α-fucosidases, consumed the majority of fucosylated HMOs tested.					
34757822	6	27	contain	possessed	911:919	arg1	pseudocatenulatum					872:888	pseudocatenulatum	872:888	pseudocatenulatum	872:888	One isolate, B. pseudocatenulatum MP80, which uniquely possessed GH95 and GH29 α-fucosidases, consumed the majority of fucosylated HMOs tested.					
34757822	6	27	contain	possessed	911:919	arg2	α-fucosidases					935:947	GH95 and GH29 α-fucosidases	921:947	GH95 and GH29 α-fucosidases	921:947	One isolate, B. pseudocatenulatum MP80, which uniquely possessed GH95 and GH29 α-fucosidases, consumed the majority of fucosylated HMOs tested.					
34757822	5	28	theme	pseudocatenulatum	829:845	arg1	strains					847:853	several sequenced B. pseudocatenulatum strains	808:853	several sequenced B. pseudocatenulatum strains	808:853	A conserved gene cluster for fucosylated HMO utilization was identified in several sequenced B. pseudocatenulatum strains.					
34757822	9	29	theme	gene	1524:1527	arg1	cluster					1529:1535	the fucosylated HMO gene cluster	1504:1535	the fucosylated HMO gene cluster in both SC585 and MP80	1504:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	16	30	theme	infant	2736:2741	arg1	gut					2743:2745	the infant gut	2732:2745	the infant gut	2732:2745	Such knowledge furthers our understanding of how diet drives bacterial colonization of the infant gut.					
34757822	4	31	theme	fucosylated	715:725	arg1	HMOs					727:730	fucosylated HMOs	715:730	fucosylated HMOs	715:730	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	7	32	theme	GH95	1071:1074	arg1	α-fucosidase					1076:1087	only a single GH95 α-fucosidase	1057:1087	only a single GH95 α-fucosidase	1057:1087	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	2	33	theme	One	234:236	arg1	species					238:244	One species	234:244	One species	234:244	One species, Bifidobacterium pseudocatenulatum, is found in the gastrointestinal tracts of adults and breastfed infants.					
34757822	2	33	theme	One	234:236	arg1	pseudocatenulatum					263:279	Bifidobacterium pseudocatenulatum	247:279	Bifidobacterium pseudocatenulatum	247:279	One species, Bifidobacterium pseudocatenulatum, is found in the gastrointestinal tracts of adults and breastfed infants.					
34757822	10	34	theme	competitive	1894:1904	arg1	behavior					1906:1913	competitive behavior	1894:1913	competitive behavior for HMO foraging in situ	1894:1938	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	7	35	theme	B.	1013:1014	arg1	SC585					1034:1038	B. pseudocatenulatum SC585	1013:1038	B. pseudocatenulatum SC585	1013:1038	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	1	36	theme	Human	158:162	arg1	milk					164:167	Human milk	158:167	Human milk	158:167	Human milk enriches members of the genus Bifidobacterium in the infant gut.					
34757822	5	37	theme	fucosylated	762:772	arg1	utilization					778:788	fucosylated HMO utilization	762:788	fucosylated HMO utilization	762:788	A conserved gene cluster for fucosylated HMO utilization was identified in several sequenced B. pseudocatenulatum strains.					
34757822	15	38	theme	complementary	2543:2555	arg1	α-fucosidases					2557:2569	complementary α-fucosidases	2543:2569	complementary α-fucosidases	2543:2569	In this case, the presence of complementary α-fucosidases may provide an advantage to microbes seeking residence in the infant gut.					
34757822	4	39	theme	milk	522:525	arg1	oligosaccharides					527:542	pooled human milk oligosaccharides	509:542	pooled human milk oligosaccharides (HMOs)	509:549	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	39	theme	milk	522:525	arg1	HMOs					545:548	HMOs	545:548	HMOs	545:548	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	14	40	theme	Bifidobacterium	2398:2412	arg1	isolates					2432:2439	Bifidobacterium pseudocatenulatum isolates	2398:2439	Bifidobacterium pseudocatenulatum isolates that may impact whether a microbe successfully colonizes an infant gut	2398:2510	This study identifies key gene differences between Bifidobacterium pseudocatenulatum isolates that may impact whether a microbe successfully colonizes an infant gut.					
34757822	3	41	theme	B.	370:371	arg1	strains					391:397	B. pseudocatenulatum strains	370:397	B. pseudocatenulatum strains	370:397	In this study, B. pseudocatenulatum strains were isolated and characterized to identify genetic adaptations to the breastfed infant gut.					
34757822	10	42	dep	presence	1741:1748	arg1	the					1737:1739	the	1737:1739	the	1737:1739	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	5	43	gly	fucosylated	762:772	arg1	utilization					778:788	fucosylated HMO utilization	762:788	fucosylated HMO utilization	762:788	A conserved gene cluster for fucosylated HMO utilization was identified in several sequenced B. pseudocatenulatum strains.					
34757822	5	44	theme	conserved	735:743	arg1	cluster					750:756	A conserved gene cluster	733:756	A conserved gene cluster for fucosylated HMO utilization	733:788	A conserved gene cluster for fucosylated HMO utilization was identified in several sequenced B. pseudocatenulatum strains.					
34757822	15	45	theme	infant	2633:2638	arg1	gut					2640:2642	the infant gut	2629:2642	the infant gut	2629:2642	In this case, the presence of complementary α-fucosidases may provide an advantage to microbes seeking residence in the infant gut.					
34757822	14	46	theme	key	2369:2371	arg1	differences					2378:2388	key gene differences	2369:2388	key gene differences between Bifidobacterium pseudocatenulatum isolates that may impact whether a microbe successfully colonizes an infant gut	2369:2510	This study identifies key gene differences between Bifidobacterium pseudocatenulatum isolates that may impact whether a microbe successfully colonizes an infant gut.					
34757822	0	47	theme	Hydrolase	123:131	arg1	Content					133:139	Glycosyl Hydrolase Content	114:139	Glycosyl Hydrolase Content	114:139	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity.					
34757822	4	48	theme	pooled	509:514	arg1	oligosaccharides					527:542	pooled human milk oligosaccharides	509:542	pooled human milk oligosaccharides (HMOs)	509:549	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	48	theme	pooled	509:514	arg1	HMOs					545:548	HMOs	545:548	HMOs	545:548	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	5	49	theme	B.	826:827	arg1	strains					847:853	several sequenced B. pseudocatenulatum strains	808:853	several sequenced B. pseudocatenulatum strains	808:853	A conserved gene cluster for fucosylated HMO utilization was identified in several sequenced B. pseudocatenulatum strains.					
34757822	13	50	theme	diverse	2293:2299	arg1	HMOs					2301:2304	structurally diverse HMOs	2280:2304	structurally diverse HMOs	2280:2304	However, catabolism of structurally diverse HMOs differs between bifidobacterial strains.					
34757822	9	51	theme	fucosylated	1610:1620	arg1	HMO					1622:1624	fucosylated HMO	1610:1624	fucosylated HMO	1610:1624	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	0	52	theme	Milk	18:21	arg1	Foraging					39:46	Fucosylated Human Milk Oligosaccharide Foraging	0:46	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum	0:99	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity.					
34757822	11	53	theme	carbohydrate	2073:2084	arg1	utilization					2086:2096	carbohydrate utilization	2073:2096	carbohydrate utilization	2073:2096	IMPORTANCE Often isolated from the human gut, microbes from the bacterial family Bifidobacteriaceae commonly possess genes enabling carbohydrate utilization.					
34757822	0	54	dep	Bifidobacterium	67:81	arg1	pseudocatenulatum					83:99	pseudocatenulatum	83:99	pseudocatenulatum	83:99	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity.					
34757822	3	55	theme	infant	480:485	arg1	gut					487:489	the breastfed infant gut	466:489	the breastfed infant gut	466:489	In this study, B. pseudocatenulatum strains were isolated and characterized to identify genetic adaptations to the breastfed infant gut.					
34757822	7	56	theme	HMO	1179:1181	arg1	pool					1183:1186	the fucosylated HMO pool	1163:1186	the fucosylated HMO pool	1163:1186	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	15	57	from	residence	2616:2624	arg1	gut					2640:2642	the infant gut	2629:2642	the infant gut	2629:2642	In this case, the presence of complementary α-fucosidases may provide an advantage to microbes seeking residence in the infant gut.					
34757822	0	58	theme	Bifidobacterium	67:81	arg1	Species					59:65	the Species	55:65	the Species Bifidobacterium pseudocatenulatum	55:99	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity.					
34757822	5	59	theme	several	808:814	arg1	strains					847:853	several sequenced B. pseudocatenulatum strains	808:853	several sequenced B. pseudocatenulatum strains	808:853	A conserved gene cluster for fucosylated HMO utilization was identified in several sequenced B. pseudocatenulatum strains.					
34757822	3	60	dep	B.	370:371	arg1	pseudocatenulatum					373:389	pseudocatenulatum	373:389	pseudocatenulatum	373:389	In this study, B. pseudocatenulatum strains were isolated and characterized to identify genetic adaptations to the breastfed infant gut.					
34757822	12	61	theme	breast	2244:2249	arg1	milk					2251:2254	human breast milk	2238:2254	human breast milk	2238:2254	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	10	62	theme	specific	1764:1771	arg1	α-fucosidases					1773:1785	specific α-fucosidases	1764:1785	specific α-fucosidases	1764:1785	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	16	63	theme	Such	2645:2648	arg1	knowledge					2650:2658	Such knowledge	2645:2658	Such knowledge	2645:2658	Such knowledge furthers our understanding of how diet drives bacterial colonization of the infant gut.					
34757822	2	64	located	found	285:289	arg1	tracts					315:320	the gastrointestinal tracts	294:320	the gastrointestinal tracts of adults and breastfed infants	294:352	One species, Bifidobacterium pseudocatenulatum, is found in the gastrointestinal tracts of adults and breastfed infants.					
34757822	2	64	located	found	285:289	arg2	pseudocatenulatum					263:279	Bifidobacterium pseudocatenulatum	247:279	Bifidobacterium pseudocatenulatum	247:279	One species, Bifidobacterium pseudocatenulatum, is found in the gastrointestinal tracts of adults and breastfed infants.					
34757822	2	64	located	found	285:289	arg2	species					238:244	One species	234:244	One species	234:244	One species, Bifidobacterium pseudocatenulatum, is found in the gastrointestinal tracts of adults and breastfed infants.					
34757822	12	65	theme	human	2238:2242	arg1	milk					2251:2254	human breast milk	2238:2254	human breast milk	2238:2254	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	9	66	theme	component	1484:1492	arg1	specificities					1431:1443	The HMO-binding specificities	1415:1443	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80	1415:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	9	66	theme	component	1484:1492	arg1	similar					1564:1570	similar	1564:1570	similar	1564:1570	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	11	67	contain	possess	2050:2056	arg2	genes					2058:2062	genes	2058:2062	genes enabling carbohydrate utilization	2058:2096	IMPORTANCE Often isolated from the human gut, microbes from the bacterial family Bifidobacteriaceae commonly possess genes enabling carbohydrate utilization.					
34757822	11	67	contain	possess	2050:2056	arg1	microbes					1987:1994	microbes	1987:1994	microbes from the bacterial family Bifidobacteriaceae	1987:2039	IMPORTANCE Often isolated from the human gut, microbes from the bacterial family Bifidobacteriaceae commonly possess genes enabling carbohydrate utilization.					
34757822	9	68	theme	HMO	1676:1678	arg1	pattern					1692:1698	each strain's distinct HMO consumption pattern	1653:1698	each strain's distinct HMO consumption pattern	1653:1698	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	7	69	theme	complete	1124:1131	arg1	repertoire					1133:1142	the complete repertoire	1120:1142	the complete repertoire of linkages within the fucosylated HMO pool	1120:1186	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	7	69	theme	complete	1124:1131	arg1	linkages					1147:1154	linkages	1147:1154	linkages within the fucosylated HMO pool	1147:1186	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	14	70	theme	infant	2501:2506	arg1	gut					2508:2510	an infant gut	2498:2510	an infant gut	2498:2510	This study identifies key gene differences between Bifidobacterium pseudocatenulatum isolates that may impact whether a microbe successfully colonizes an infant gut.					
34757822	10	71	theme	utilization	1831:1841	arg1	pattern					1843:1849	the strain-specific fucosylated HMO utilization pattern	1795:1849	the strain-specific fucosylated HMO utilization pattern	1795:1849	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	7	72	contain	possesses	1047:1055	arg2	α-fucosidase					1076:1087	only a single GH95 α-fucosidase	1057:1087	only a single GH95 α-fucosidase	1057:1087	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	7	72	contain	possesses	1047:1055	arg1	SC585					1034:1038	B. pseudocatenulatum SC585	1013:1038	B. pseudocatenulatum SC585	1013:1038	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	10	73	dep	in	1932:1933	arg1	situ					1935:1938	situ	1935:1938	situ	1935:1938	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	10	74	theme	α-fucosidases	1773:1785	arg1	absence					1753:1759	absence	1753:1759	absence	1753:1759	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	10	74	theme	α-fucosidases	1773:1785	arg1	presence					1741:1748	presence	1741:1748	presence	1741:1748	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	9	75	theme	protein	1476:1482	arg1	component					1484:1492	the family 1 solute-binding protein component	1448:1492	the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80	1448:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	2	76	theme	breastfed	336:344	arg1	infants					346:352	breastfed infants	336:352	breastfed infants	336:352	One species, Bifidobacterium pseudocatenulatum, is found in the gastrointestinal tracts of adults and breastfed infants.					
34757822	1	77	theme	Bifidobacterium	199:213	arg1	members					178:184	members	178:184	members of the genus Bifidobacterium	178:213	Human milk enriches members of the genus Bifidobacterium in the infant gut.					
34757822	9	78	from	cluster	1529:1535	arg1	MP80					1555:1558	MP80	1555:1558	MP80	1555:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	9	78	from	cluster	1529:1535	arg1	SC585					1545:1549	SC585	1545:1549	SC585	1545:1549	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	2	79	theme	adults	325:330	arg1	tracts					315:320	the gastrointestinal tracts	294:320	the gastrointestinal tracts of adults and breastfed infants	294:352	One species, Bifidobacterium pseudocatenulatum, is found in the gastrointestinal tracts of adults and breastfed infants.					
34757822	8	80	theme	GH29	1214:1217	arg1	activities					1239:1248	GH95 fucosidase activities	1223:1248	GH95 fucosidase activities directly on HMOs	1223:1265	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	8	80	theme	GH29	1214:1217	arg1	Analysis					1189:1196	Analysis	1189:1196	Analysis of the purified GH29	1189:1217	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	6	81	theme	HMOs	987:990	arg1	majority					963:970	the majority	959:970	the majority of fucosylated HMOs tested	959:997	One isolate, B. pseudocatenulatum MP80, which uniquely possessed GH95 and GH29 α-fucosidases, consumed the majority of fucosylated HMOs tested.					
34757822	12	82	contain	possess	2149:2155	arg2	glycans					2221:2227	glycans	2221:2227	glycans found in human breast milk	2221:2254	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	12	82	contain	possess	2149:2155	arg1	Isolates					2099:2106	Isolates	2099:2106	Isolates from breastfed infants	2099:2129	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	12	82	contain	possess	2149:2155	arg2	genes					2157:2161	genes	2157:2161	genes for the catabolism of human milk oligosaccharides	2157:2211	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	8	83	theme	enzyme	1290:1295	arg1	specificities					1297:1309	complementing enzyme specificities	1276:1309	complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages	1276:1412	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	11	84	theme	family	2015:2020	arg1	Bifidobacteriaceae					2022:2039	the bacterial family Bifidobacteriaceae	2001:2039	the bacterial family Bifidobacteriaceae	2001:2039	IMPORTANCE Often isolated from the human gut, microbes from the bacterial family Bifidobacteriaceae commonly possess genes enabling carbohydrate utilization.					
34757822	9	85	theme	HMO	1520:1522	arg1	cluster					1529:1535	the fucosylated HMO gene cluster	1504:1535	the fucosylated HMO gene cluster in both SC585 and MP80	1504:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	12	86	located	found	2229:2233	arg1	milk					2251:2254	human breast milk	2238:2254	human breast milk	2238:2254	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	12	86	located	found	2229:2233	arg2	glycans					2221:2227	glycans	2221:2227	glycans found in human breast milk	2221:2254	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	16	87	theme	gut	2743:2745	arg1	colonization					2716:2727	bacterial colonization	2706:2727	bacterial colonization of the infant gut	2706:2745	Such knowledge furthers our understanding of how diet drives bacterial colonization of the infant gut.					
34757822	12	88	theme	milk	2191:2194	arg1	oligosaccharides					2196:2211	human milk oligosaccharides	2185:2211	human milk oligosaccharides	2185:2211	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	7	89	theme	single	1064:1069	arg1	α-fucosidase					1076:1087	only a single GH95 α-fucosidase	1057:1087	only a single GH95 α-fucosidase	1057:1087	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	9	90	gly	fucosylated	1508:1518	arg1	cluster					1529:1535	the fucosylated HMO gene cluster	1504:1535	the fucosylated HMO gene cluster in both SC585 and MP80	1504:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	8	91	theme	GH95	1320:1323	arg1	enzyme					1325:1330	the GH95 enzyme	1316:1330	the GH95 enzyme preferring 1-2 fucosyl linkages	1316:1362	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	10	92	theme	fucosylated	1815:1825	arg1	pattern					1843:1849	the strain-specific fucosylated HMO utilization pattern	1795:1849	the strain-specific fucosylated HMO utilization pattern	1795:1849	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	4	93	theme	HMOs	727:730	arg1	catabolism					701:710	variable catabolism	692:710	variable catabolism of fucosylated HMOs	692:730	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	12	94	theme	human	2185:2189	arg1	oligosaccharides					2196:2211	human milk oligosaccharides	2185:2211	human milk oligosaccharides	2185:2211	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	13	95	theme	bifidobacterial	2322:2336	arg1	strains					2338:2344	bifidobacterial strains	2322:2344	bifidobacterial strains	2322:2344	However, catabolism of structurally diverse HMOs differs between bifidobacterial strains.					
34757822	8	96	theme	fucosyl	1347:1353	arg1	linkages					1355:1362	1-2 fucosyl linkages	1343:1362	1-2 fucosyl linkages	1343:1362	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	14	97	theme	pseudocatenulatum	2414:2430	arg1	isolates					2432:2439	Bifidobacterium pseudocatenulatum isolates	2398:2439	Bifidobacterium pseudocatenulatum isolates that may impact whether a microbe successfully colonizes an infant gut	2398:2510	This study identifies key gene differences between Bifidobacterium pseudocatenulatum isolates that may impact whether a microbe successfully colonizes an infant gut.					
34757822	5	98	theme	HMO	774:776	arg1	utilization					778:788	fucosylated HMO utilization	762:788	fucosylated HMO utilization	762:788	A conserved gene cluster for fucosylated HMO utilization was identified in several sequenced B. pseudocatenulatum strains.					
34757822	15	99	theme	α-fucosidases	2557:2569	arg1	presence					2531:2538	the presence	2527:2538	the presence of complementary α-fucosidases	2527:2569	In this case, the presence of complementary α-fucosidases may provide an advantage to microbes seeking residence in the infant gut.					
34757822	4	100	theme	variable	692:699	arg1	catabolism					701:710	variable catabolism	692:710	variable catabolism of fucosylated HMOs	692:730	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	0	101	theme	Glycosyl	114:121	arg1	Content					133:139	Glycosyl Hydrolase Content	114:139	Glycosyl Hydrolase Content	114:139	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity.					
34757822	12	102	dep	possess	2149:2155	arg1	HMOs					2214:2217	HMOs	2214:2217	HMOs	2214:2217	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	9	103	attach	linked	1494:1499	arg1	cluster					1529:1535	the fucosylated HMO gene cluster	1504:1535	the fucosylated HMO gene cluster in both SC585 and MP80	1504:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	9	103	attach	linked	1494:1499	arg2	component					1484:1492	the family 1 solute-binding protein component	1448:1492	the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80	1448:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	8	104	theme	GH29	1372:1375	arg1	enzyme					1377:1382	the GH29 enzyme	1368:1382	the GH29 enzyme favoring 1-3 and 1-4 linkages	1368:1412	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	14	105	theme	gene	2373:2376	arg1	differences					2378:2388	key gene differences	2369:2388	key gene differences between Bifidobacterium pseudocatenulatum isolates that may impact whether a microbe successfully colonizes an infant gut	2369:2510	This study identifies key gene differences between Bifidobacterium pseudocatenulatum isolates that may impact whether a microbe successfully colonizes an infant gut.					
34757822	5	106	theme	gene	745:748	arg1	cluster					750:756	A conserved gene cluster	733:756	A conserved gene cluster for fucosylated HMO utilization	733:788	A conserved gene cluster for fucosylated HMO utilization was identified in several sequenced B. pseudocatenulatum strains.					
34757822	9	107	theme	fucosylated	1508:1518	arg1	cluster					1529:1535	the fucosylated HMO gene cluster	1504:1535	the fucosylated HMO gene cluster in both SC585 and MP80	1504:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	9	108	theme	differential	1584:1595	arg1	factor					1643:1648	a driving factor	1633:1648	a driving factor in each strain's distinct HMO consumption pattern	1633:1698	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	9	108	theme	differential	1584:1595	arg1	transport					1597:1605	differential transport	1584:1605	differential transport of fucosylated HMO	1584:1624	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	4	109	theme	human	516:520	arg1	oligosaccharides					527:542	pooled human milk oligosaccharides	509:542	pooled human milk oligosaccharides (HMOs)	509:549	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	109	theme	human	516:520	arg1	HMOs					545:548	HMOs	545:548	HMOs	545:548	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	3	110	theme	breastfed	470:478	arg1	gut					487:489	the breastfed infant gut	466:489	the breastfed infant gut	466:489	In this study, B. pseudocatenulatum strains were isolated and characterized to identify genetic adaptations to the breastfed infant gut.					
34757822	0	111	theme	Human	12:16	arg1	Foraging					39:46	Fucosylated Human Milk Oligosaccharide Foraging	0:46	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum	0:99	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity.					
34757822	4	112	theme	distinct	568:575	arg1	difference					671:680	a difference	669:680	a difference driven by variable catabolism of fucosylated HMOs	669:730	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	112	theme	distinct	568:575	arg1	isolates					609:616	isolates	609:616	isolates that readily consumed HMOs	609:643	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	112	theme	distinct	568:575	arg1	groups					577:582	two distinct groups	564:582	two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not	564:666	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	112	theme	distinct	568:575	arg1	pseudocatenulatum					590:606	B. pseudocatenulatum	587:606	B. pseudocatenulatum	587:606	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	4	112	theme	distinct	568:575	arg1	those					649:653	those	649:653	those	649:653	During growth on pooled human milk oligosaccharides (HMOs), we observed two distinct groups of B. pseudocatenulatum, isolates that readily consumed HMOs and those that did not, a difference driven by variable catabolism of fucosylated HMOs.					
34757822	0	113	theme	Oligosaccharide	23:37	arg1	Foraging					39:46	Fucosylated Human Milk Oligosaccharide Foraging	0:46	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum	0:99	Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity.					
34757822	13	114	theme	HMOs	2301:2304	arg1	catabolism					2266:2275	catabolism	2266:2275	catabolism of structurally diverse HMOs	2266:2304	However, catabolism of structurally diverse HMOs differs between bifidobacterial strains.					
34757822	9	115	theme	HMO	1622:1624	arg1	factor					1643:1648	a driving factor	1633:1648	a driving factor in each strain's distinct HMO consumption pattern	1633:1698	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	9	115	theme	HMO	1622:1624	arg1	transport					1597:1605	differential transport	1584:1605	differential transport of fucosylated HMO	1584:1624	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	9	116	from	factor	1643:1648	arg1	pattern					1692:1698	each strain's distinct HMO consumption pattern	1653:1698	each strain's distinct HMO consumption pattern	1653:1698	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	10	117	theme	in	1932:1933	arg1	foraging					1923:1930	HMO foraging	1919:1930	HMO foraging in situ	1919:1938	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	7	118	dep	B.	1013:1014	arg1	pseudocatenulatum					1016:1032	pseudocatenulatum	1016:1032	pseudocatenulatum	1016:1032	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	7	119	theme	fucosylated	1167:1177	arg1	pool					1183:1186	the fucosylated HMO pool	1163:1186	the fucosylated HMO pool	1163:1186	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	10	120	gly	fucosylated	1815:1825	arg1	pattern					1843:1849	the strain-specific fucosylated HMO utilization pattern	1795:1849	the strain-specific fucosylated HMO utilization pattern	1795:1849	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	12	121	theme	oligosaccharides	2196:2211	arg1	catabolism					2171:2180	the catabolism	2167:2180	the catabolism of human milk oligosaccharides	2167:2211	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	3	122	theme	genetic	443:449	arg1	adaptations					451:461	genetic adaptations	443:461	genetic adaptations to the breastfed infant gut	443:489	In this study, B. pseudocatenulatum strains were isolated and characterized to identify genetic adaptations to the breastfed infant gut.					
34757822	9	123	gly	fucosylated	1610:1620	arg1	HMO					1622:1624	fucosylated HMO	1610:1624	fucosylated HMO	1610:1624	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	10	124	theme	HMO	1919:1921	arg1	foraging					1923:1930	HMO foraging	1919:1930	HMO foraging in situ	1919:1938	Taken together, these data indicate the presence or absence of specific α-fucosidases directs the strain-specific fucosylated HMO utilization pattern among bifidobacteria and likely influences competitive behavior for HMO foraging in situ.					
34757822	7	125	gly	fucosylated	1167:1177	arg1	pool					1183:1186	the fucosylated HMO pool	1163:1186	the fucosylated HMO pool	1163:1186	Furthermore, B. pseudocatenulatum SC585, which possesses only a single GH95 α-fucosidase, lacked the ability to consume the complete repertoire of linkages within the fucosylated HMO pool.					
34757822	9	126	theme	driving	1635:1641	arg1	transport					1597:1605	differential transport	1584:1605	differential transport of fucosylated HMO	1584:1624	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	9	126	theme	driving	1635:1641	arg1	factor					1643:1648	a driving factor	1633:1648	a driving factor in each strain's distinct HMO consumption pattern	1633:1698	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	8	127	from	Analysis	1189:1196	arg1	HMOs					1262:1265	HMOs	1262:1265	HMOs	1262:1265	Analysis of the purified GH29 and GH95 fucosidase activities directly on HMOs revealed complementing enzyme specificities with the GH95 enzyme preferring 1-2 fucosyl linkages and the GH29 enzyme favoring 1-3 and 1-4 linkages.					
34757822	2	128	theme	infants	346:352	arg1	tracts					315:320	the gastrointestinal tracts	294:320	the gastrointestinal tracts of adults and breastfed infants	294:352	One species, Bifidobacterium pseudocatenulatum, is found in the gastrointestinal tracts of adults and breastfed infants.					
34757822	9	129	theme	HMO-binding	1419:1429	arg1	specificities					1431:1443	The HMO-binding specificities	1415:1443	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80	1415:1558	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	9	129	theme	HMO-binding	1419:1429	arg1	similar					1564:1570	similar	1564:1570	similar	1564:1570	The HMO-binding specificities of the family 1 solute-binding protein component linked to the fucosylated HMO gene cluster in both SC585 and MP80 are similar, suggesting differential transport of fucosylated HMO is not a driving factor in each strain's distinct HMO consumption pattern.					
34757822	12	130	theme	breastfed	2113:2121	arg1	infants					2123:2129	breastfed infants	2113:2129	breastfed infants	2113:2129	Isolates from breastfed infants often grow on and possess genes for the catabolism of human milk oligosaccharides (HMOs), glycans found in human breast milk.					
34757822	11	131	theme	human	1976:1980	arg1	gut					1982:1984	the human gut	1972:1984	the human gut	1972:1984	IMPORTANCE Often isolated from the human gut, microbes from the bacterial family Bifidobacteriaceae commonly possess genes enabling carbohydrate utilization.					
34757822	5	132	theme	sequenced	816:824	arg1	strains					847:853	several sequenced B. pseudocatenulatum strains	808:853	several sequenced B. pseudocatenulatum strains	808:853	A conserved gene cluster for fucosylated HMO utilization was identified in several sequenced B. pseudocatenulatum strains.					
34716519	0	0	theme	cadmium	94:100	arg1	cations					107:113	cadmium (II) cations	94:113	cadmium (II) cations	94:113	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.					
34716519	3	1	theme	concentration	464:476	arg1	influences					437:446	The influences	433:446	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms	433:601	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	7	2	theme	cadmium	1384:1390	arg1	removal					1392:1398	cadmium removal	1384:1398	cadmium removal from both the soil and wastewater systems	1384:1440	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	4	3	theme	cadmium	657:663	arg1	capacity					645:652	The maximum adsorption capacity	622:652	The maximum adsorption capacity of cadmium onto CSA	622:672	The maximum adsorption capacity of cadmium onto CSA was significantly increased from 2264.9 to 4380.97 μmol/g when the system was changed from soil to water, respectively.					
34716519	7	4	theme	Δq	1135:1136	arg1	amounts					1188:1194	desorption amounts	1177:1194	desorption amounts	1177:1194	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	7	4	theme	Δq	1135:1136	arg1	difference					1143:1152	the difference	1139:1152	the difference between adsorption	1139:1171	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	7	4	theme	Δq	1135:1136	arg1	amounts					1124:1130	the amounts	1120:1130	the amounts of Δq	1120:1136	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	7	4	theme	Δq	1135:1136	arg1	Δq					1135:1136	Δq	1135:1136	Δq	1135:1136	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	3	5	theme	cellulose	500:508	arg1	strength					555:562	ionic strength	549:562	ionic strength	549:562	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	3	5	theme	cellulose	500:508	arg1	type					511:514	type	511:514	type of the polluted environment	511:542	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	3	5	theme	cellulose	500:508	arg1	concentration					464:476	initial Cd2+ concentration	451:476	initial Cd2+ concentration	451:476	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	3	5	theme	cellulose	500:508	arg1	presence					483:490	the presence	479:490	the presence of nano cellulose	479:508	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	6	6	theme	Langmuir	984:991	arg1	models					1008:1013	Langmuir and Freundlich models	984:1013	Langmuir and Freundlich models	984:1013	The results indicated that Langmuir and Freundlich models provided the best fit for the experimental adsorption data for CSA and CSA-N, respectively.					
34716519	1	7	theme	soil	337:340	arg1	systems					342:348	soil systems	337:348	soil systems	337:348	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	1	8	theme	green	269:273	arg1	route					275:279	a completely green route	256:279	a completely green route	256:279	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	0	9	theme	cations	107:113	arg1	removal					83:89	the efficient removal	69:89	the efficient removal of cadmium (II) cations from wastewater and soil systems	69:146	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.					
34716519	1	10	used	used	285:288	arg2	sorbents					293:300	sorbents	293:300	sorbents	293:300	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	1	10	used	used	285:288	arg2	hydrogels					226:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	6	11	theme	best	1028:1031	arg1	fit					1033:1035	the best fit	1024:1035	the best fit for the experimental adsorption data for CSA and CSA-N	1024:1090	The results indicated that Langmuir and Freundlich models provided the best fit for the experimental adsorption data for CSA and CSA-N, respectively.					
34716519	7	12	dep	efficient	1351:1359	arg1	adsorbent					1361:1369	adsorbent	1361:1369	adsorbent	1361:1369	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	4	13	theme	adsorption	634:643	arg1	capacity					645:652	The maximum adsorption capacity	622:652	The maximum adsorption capacity of cadmium onto CSA	622:672	The maximum adsorption capacity of cadmium onto CSA was significantly increased from 2264.9 to 4380.97 μmol/g when the system was changed from soil to water, respectively.					
34716519	5	14	theme	adsorption	813:822	arg1	capacity					824:831	the maximum adsorption capacity	801:831	the maximum adsorption capacity of cadmium onto CSA-N	801:853	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	5	14	theme	adsorption	813:822	arg1	same					870:873	same	870:873	same	870:873	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	0	15	from	systems	140:146	arg1	removal					83:89	the efficient removal	69:89	the efficient removal of cadmium (II) cations from wastewater and soil systems	69:146	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.					
34716519	3	16	from	influences	437:446	arg1	adsorption					567:576	adsorption	567:576	adsorption	567:576	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	3	16	from	influences	437:446	arg1	isotherms					593:601	desorption isotherms	582:601	desorption isotherms	582:601	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	3	17	theme	nano	495:498	arg1	cellulose					500:508	nano cellulose	495:508	nano cellulose	495:508	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	4	18	dep	4380.97 μmol/g	717:730	arg1	to					714:715	to	714:715	to	714:715	The maximum adsorption capacity of cadmium onto CSA was significantly increased from 2264.9 to 4380.97 μmol/g when the system was changed from soil to water, respectively.					
34716519	5	19	theme	soil	882:885	arg1	systems					902:908	the soil and wastewater systems	878:908	systems	902:908	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	1	20	theme	chitosan/sodium	164:178	arg1	hydrogels					226:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	1	20	theme	chitosan/sodium	164:178	arg1	sorbents					293:300	sorbents	293:300	sorbents	293:300	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	3	21	theme	polluted	523:530	arg1	environment					532:542	the polluted environment	519:542	the polluted environment	519:542	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	7	22	theme	feasible	1226:1233	arg1	sorbent					1235:1241	economically feasible sorbent	1213:1241	economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system	1213:1305	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	7	22	theme	feasible	1226:1233	arg1	CSA					1201:1203	the CSA	1197:1203	the CSA	1197:1203	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	1	23	theme	alginate/nano	180:192	arg1	hydrogels					226:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	1	23	theme	alginate/nano	180:192	arg1	sorbents					293:300	sorbents	293:300	sorbents	293:300	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	0	24	theme	alginate/nano	31:43	arg1	composite					55:63	chitosan/sodium alginate/nano cellulose composite	15:63	chitosan/sodium alginate/nano cellulose composite	15:63	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.					
34716519	7	25	theme	Cd2+	1277:1280	arg1	concentrations					1259:1272	high initial concentrations	1246:1272	high initial concentrations of Cd2+	1246:1280	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	5	26	from	same	870:873	arg1	systems					902:908	the soil and wastewater systems	878:908	systems	902:908	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	3	27	theme	type	511:514	arg1	influences					437:446	The influences	433:446	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms	433:601	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	1	28	theme	cellulose	194:202	arg1	hydrogels					226:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	1	28	theme	cellulose	194:202	arg1	sorbents					293:300	sorbents	293:300	sorbents	293:300	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	0	29	theme	chitosan/sodium	15:29	arg1	composite					55:63	chitosan/sodium alginate/nano cellulose composite	15:63	chitosan/sodium alginate/nano cellulose composite	15:63	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.					
34716519	1	30	theme	environmental	356:368	arg1	aspect					370:375	an environmental aspect	353:375	an environmental aspect	353:375	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	7	31	theme	desorption	1177:1186	arg1	amounts					1188:1194	desorption amounts	1177:1194	desorption amounts	1177:1194	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	7	31	theme	desorption	1177:1186	arg1	Δq					1135:1136	Δq	1135:1136	Δq	1135:1136	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	0	32	theme	composite	55:63	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.	0:147	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.					
34716519	5	33	dep	3419.5	917:922	arg1	i.e.					911:914	i.e.	911:914	i.e.	911:914	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	0	34	theme	soil	135:138	arg1	systems					140:146	soil systems	135:146	soil systems	135:146	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.					
34716519	1	35	theme	aspect	370:375	arg1	water					327:331	water	327:331	water	327:331	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	1	35	theme	aspect	370:375	arg1	systems					342:348	soil systems	337:348	soil systems	337:348	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	4	36	theme	maximum	626:632	arg1	capacity					645:652	The maximum adsorption capacity	622:652	The maximum adsorption capacity of cadmium onto CSA	622:672	The maximum adsorption capacity of cadmium onto CSA was significantly increased from 2264.9 to 4380.97 μmol/g when the system was changed from soil to water, respectively.					
34716519	1	37	theme	CSA-N	205:209	arg1	hydrogels					226:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	1	37	theme	CSA-N	205:209	arg1	sorbents					293:300	sorbents	293:300	sorbents	293:300	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	0	38	theme	cellulose	45:53	arg1	composite					55:63	chitosan/sodium alginate/nano cellulose composite	15:63	chitosan/sodium alginate/nano cellulose composite	15:63	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.					
34716519	3	39	theme	desorption	582:591	arg1	isotherms					593:601	desorption isotherms	582:601	desorption isotherms	582:601	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	3	40	theme	ionic	549:553	arg1	strength					555:562	ionic strength	549:562	ionic strength	549:562	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	5	41	theme	maximum	805:811	arg1	capacity					824:831	the maximum adsorption capacity	801:831	the maximum adsorption capacity of cadmium onto CSA-N	801:853	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	5	41	theme	maximum	805:811	arg1	same					870:873	same	870:873	same	870:873	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	7	42	from	systems	1434:1440	arg1	removal					1392:1398	cadmium removal	1384:1398	cadmium removal from both the soil and wastewater systems	1384:1440	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	3	43	theme	environment	532:542	arg1	strength					555:562	ionic strength	549:562	ionic strength	549:562	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	3	43	theme	environment	532:542	arg1	type					511:514	type	511:514	type of the polluted environment	511:542	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	3	43	theme	environment	532:542	arg1	concentration					464:476	initial Cd2+ concentration	451:476	initial Cd2+ concentration	451:476	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	3	43	theme	environment	532:542	arg1	presence					483:490	the presence	479:490	the presence of nano cellulose	479:508	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	3	44	theme	initial	451:457	arg1	concentration					464:476	initial Cd2+ concentration	451:476	initial Cd2+ concentration	451:476	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	7	45	theme	initial	1251:1257	arg1	concentrations					1259:1272	high initial concentrations	1246:1272	high initial concentrations of Cd2+	1246:1280	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	7	46	theme	wastewater	1423:1432	arg1	systems					1434:1440	both the soil and wastewater systems	1405:1440	systems	1434:1440	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	7	47	from	sorbent	1235:1241	arg1	system					1300:1305	the wastewater system	1285:1305	the wastewater system	1285:1305	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	3	48	theme	Cd2+	459:462	arg1	concentration					464:476	initial Cd2+ concentration	451:476	initial Cd2+ concentration	451:476	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	1	49	theme	Cd2+	312:315	arg1	ions					317:320	Cd2+ ions	312:320	Cd2+ ions	312:320	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	5	50	theme	cadmium	836:842	arg1	capacity					824:831	the maximum adsorption capacity	801:831	the maximum adsorption capacity of cadmium onto CSA-N	801:853	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	5	50	theme	cadmium	836:842	arg1	same					870:873	same	870:873	same	870:873	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	5	51	theme	wastewater	891:900	arg1	systems					902:908	the soil and wastewater systems	878:908	systems	902:908	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	1	52	theme	nanocomposite	212:224	arg1	hydrogels					226:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels	164:234	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	1	52	theme	nanocomposite	212:224	arg1	sorbents					293:300	sorbents	293:300	sorbents	293:300	In this study, chitosan/sodium alginate/nano cellulose (CSA-N) nanocomposite hydrogels were prepared using a completely green route and used as sorbents to adsorb Cd2+ ions from water and soil systems of an environmental aspect.					
34716519	7	53	theme	wastewater	1289:1298	arg1	system					1300:1305	the wastewater system	1285:1305	the wastewater system	1285:1305	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	6	54	theme	adsorption	1058:1067	arg1	data					1069:1072	the experimental adsorption data	1041:1072	the experimental adsorption data	1041:1072	The results indicated that Langmuir and Freundlich models provided the best fit for the experimental adsorption data for CSA and CSA-N, respectively.					
34716519	7	55	theme	high	1246:1249	arg1	concentrations					1259:1272	high initial concentrations	1246:1272	high initial concentrations of Cd2+	1246:1280	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	7	56	theme	soil	1414:1417	arg1	systems					1434:1440	both the soil and wastewater systems	1405:1440	systems	1434:1440	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	3	57	theme	strength	555:562	arg1	influences					437:446	The influences	433:446	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms	433:601	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
34716519	6	58	theme	Freundlich	997:1006	arg1	models					1008:1013	Langmuir and Freundlich models	984:1013	Langmuir and Freundlich models	984:1013	The results indicated that Langmuir and Freundlich models provided the best fit for the experimental adsorption data for CSA and CSA-N, respectively.					
34716519	6	59	theme	experimental	1045:1056	arg1	data					1069:1072	the experimental adsorption data	1041:1072	the experimental adsorption data	1041:1072	The results indicated that Langmuir and Freundlich models provided the best fit for the experimental adsorption data for CSA and CSA-N, respectively.					
34716519	5	60	from	systems	902:908	arg1	same					870:873	same	870:873	same	870:873	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	5	60	from	systems	902:908	arg1	capacity					824:831	the maximum adsorption capacity	801:831	the maximum adsorption capacity of cadmium onto CSA-N	801:853	While, the maximum adsorption capacity of cadmium onto CSA-N was almost the same in the soil and wastewater systems, i.e., 3419.5 and 3230.3 µmol/g, respectively.					
34716519	0	61	from	wastewater	120:129	arg1	removal					83:89	the efficient removal	69:89	the efficient removal of cadmium (II) cations from wastewater and soil systems	69:146	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.					
34716519	0	62	theme	efficient	73:81	arg1	removal					83:89	the efficient removal	69:89	the efficient removal of cadmium (II) cations from wastewater and soil systems	69:146	Preparation of chitosan/sodium alginate/nano cellulose composite for the efficient removal of cadmium (II) cations from wastewater and soil systems.					
34716519	7	63	from	concentrations	1259:1272	arg1	sorbent					1235:1241	economically feasible sorbent	1213:1241	economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system	1213:1305	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	7	63	from	concentrations	1259:1272	arg1	CSA					1201:1203	the CSA	1197:1203	the CSA	1197:1203	By comparing the amounts of Δq, the difference between adsorption and desorption amounts, the CSA was not economically feasible sorbent at high initial concentrations of Cd2+ in the wastewater system, while, CSA-N was demonstrated to be a more efficient adsorbent than CSA for cadmium removal from both the soil and wastewater systems.					
34716519	3	64	theme	presence	483:490	arg1	influences					437:446	The influences	433:446	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms	433:601	The influences of initial Cd2+ concentration, the presence of nano cellulose, type of the polluted environment, and ionic strength on adsorption and desorption isotherms were investigated.					
33358299	5	0	with	samples	1283:1289	arg1	density					1313:1319	a overall charge density	1296:1319	a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5	1296:1353	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	5	1	theme	0.92-0.93	1324:1332	arg1	density					1313:1319	a overall charge density	1296:1319	a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5	1296:1353	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	5	2	theme	located	1136:1142	arg1	groups					1124:1129	the sulfate groups	1112:1129	the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio)	1112:1200	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	2	3	theme	variable	623:630	arg1	structure					632:640	variable structure	623:640	variable structure	623:640	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	4	4	theme	polydispersity	1053:1066	arg1	variability					1068:1078	a polydispersity variability	1051:1078	a polydispersity variability of 0.7-2.7 %	1051:1091	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	3	5	theme	animal	753:758	arg1	tissues					760:766	standardized source animal tissues	733:766	standardized source animal tissues	733:766	By using standardized source animal tissues and manufacturing procedure, highly pure CS (∼92 %) products with constant structure and characteristics were obtained.					
33358299	5	6	theme	overall	1298:1304	arg1	density					1313:1319	a overall charge density	1296:1319	a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5	1296:1353	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	2	7	with	products	609:616	arg1	structure					632:640	variable structure	623:640	variable structure	623:640	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	2	7	with	products	609:616	arg1	contaminants					657:668	biological contaminants	646:668	biological contaminants	646:668	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	6	8	theme	macro	1460:1464	arg1	molecules					1466:1474	other co-purified bio(macro)molecules	1438:1474	other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins)	1438:1572	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	8	theme	macro	1460:1464	arg1	sodium					1587:1592	sodium	1587:1592	sodium	1587:1592	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	8	theme	macro	1460:1464	arg1	calcium					1575:1581	calcium	1575:1581	calcium	1575:1581	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	4	9	theme	molecular	913:921	arg1	weight					923:928	a lower molecular weight	905:928	a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products	905:1022	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	4	10	theme	CV	1031:1032	arg1	∼2.0-7.5					1038:1045	∼2.0-7.5	1038:1045	∼2.0-7.5	1038:1045	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	4	10	theme	CV	1031:1032	arg1	%					1033:1033	a CV%	1029:1033	a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %	1029:1091	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	4	10	theme	CV	1031:1032	arg1	variability					1068:1078	a polydispersity variability	1051:1078	a polydispersity variability of 0.7-2.7 %	1051:1091	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	3	11	theme	standardized	733:744	arg1	tissues					760:766	standardized source animal tissues	733:766	standardized source animal tissues	733:766	By using standardized source animal tissues and manufacturing procedure, highly pure CS (∼92 %) products with constant structure and characteristics were obtained.					
33358299	5	12	from	located	1136:1142	arg1	position					1147:1154	position 4 and 6	1147:1162	position 4 and 6 of N-acetyl-galactosamine (4/6 ratio)	1147:1200	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	1	13	theme	sulfate	214:220	arg1	samples					227:233	chondroitin sulfate (CS) samples	202:233	chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	202:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	6	14	theme	heparan	1529:1535	arg1	acid					1488:1491	hyaluronic acid	1477:1491	hyaluronic acid	1477:1491	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	14	theme	heparan	1529:1535	arg1	sulfate					1537:1543	heparan sulfate	1529:1543	heparan sulfate	1529:1543	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	9	15	theme	norm	2029:2032	arg1	measure					2014:2020	a measure	2012:2020	a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared	2012:2215	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	6	16	theme	bio	1456:1458	arg1	molecules					1466:1474	other co-purified bio(macro)molecules	1438:1474	other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins)	1438:1572	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	16	theme	bio	1456:1458	arg1	sodium					1587:1592	sodium	1587:1592	sodium	1587:1592	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	16	theme	bio	1456:1458	arg1	calcium					1575:1581	calcium	1575:1581	calcium	1575:1581	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	2	17	theme	controlled	558:567	arg1	procedures					580:589	not controlled extractive procedures	554:589	not controlled extractive procedures	554:589	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	10	18	theme	quality	2423:2429	arg1	CS					2412:2413	CS	2412:2413	CS of high quality	2412:2429	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	9	19	theme	range	2038:2042	arg1	measure					2014:2020	a measure	2012:2020	a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared	2012:2215	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	4	20	dep	porcine	955:961	arg1	MWw					964:966	MWw	964:966	MWw of ∼26,000 Da	964:980	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	1	21	attach	derived	235:241	arg1	sources					294:300	three largely available and common biological sources	248:300	three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	248:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	21	attach	derived	235:241	arg1	bones					354:358	bovine and porcine trachea and chicken keel bones	310:358	bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	310:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	21	attach	derived	235:241	arg2	samples					227:233	chondroitin sulfate (CS) samples	202:233	chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	202:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	6	22	theme	other	1438:1442	arg1	molecules					1466:1474	other co-purified bio(macro)molecules	1438:1474	other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins)	1438:1572	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	22	theme	other	1438:1442	arg1	sodium					1587:1592	sodium	1587:1592	sodium	1587:1592	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	22	theme	other	1438:1442	arg1	calcium					1575:1581	calcium	1575:1581	calcium	1575:1581	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	2	23	theme	constant	685:692	arg1	efficacy					703:710	constant clinical efficacy	685:710	constant clinical efficacy	685:710	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	3	24	theme	CS	809:810	arg1	products					820:827	highly pure CS (∼92 %) products	797:827	highly pure CS (∼92 %) products with constant structure and characteristics	797:871	By using standardized source animal tissues and manufacturing procedure, highly pure CS (∼92 %) products with constant structure and characteristics were obtained.					
33358299	5	25	from	position	1147:1154	arg1	located					1136:1142	located	1136:1142	located	1136:1142	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	6	26	theme	content	1427:1433	arg1	absence					1603:1609	the absence	1599:1609	the absence of versican	1599:1621	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	26	theme	content	1427:1433	arg1	presence					1391:1398	the presence	1387:1398	the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium,	1387:1593	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	1	27	with	bones	354:358	arg1	aim					369:371	the aim to define their structural signatures	365:409	the aim to define their structural signatures	365:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	8	28	theme	CS	1869:1870	arg1	origin					1859:1864	the origin	1855:1864	the origin of CS	1855:1870	The observed parameters are structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process.					
33358299	6	29	theme	keratan	1494:1500	arg1	acid					1488:1491	hyaluronic acid	1477:1491	hyaluronic acid	1477:1491	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	29	theme	keratan	1494:1500	arg1	sulfate					1502:1508	keratan sulfate	1494:1508	keratan sulfate	1494:1508	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	10	30	theme	pharmaceutical/nutraceutical	2450:2477	arg1	agents					2486:2491	pharmaceutical/nutraceutical active agents	2450:2491	pharmaceutical/nutraceutical active agents	2450:2491	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	9	31	theme	CS	2060:2061	arg1	samples					2063:2069	CS samples	2060:2069	CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared	2060:2215	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	0	32	theme	origin	68:73	arg1	sulfate					49:55	terrestrial chondroitin sulfate	25:55	terrestrial chondroitin sulfate of various origin	25:73	Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process.					
33358299	9	33	theme	variation	2047:2055	arg1	range					2038:2042	range	2038:2042	range	2038:2042	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	9	33	theme	variation	2047:2055	arg1	norm					2029:2032	norm	2029:2032	norm	2029:2032	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	10	34	theme	physicochemical	2232:2246	arg1	properties					2248:2257	the physicochemical properties	2228:2257	the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension	2228:2367	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	10	34	theme	physicochemical	2232:2246	arg1	weight					2279:2284	molecular weight	2269:2284	molecular weight	2269:2284	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	10	34	theme	physicochemical	2232:2246	arg1	dimension					2359:2367	particle dimension	2350:2367	particle dimension	2350:2367	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	10	34	theme	physicochemical	2232:2246	arg1	composition					2300:2310	disaccharide composition	2287:2310	disaccharide composition	2287:2310	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	10	34	theme	physicochemical	2232:2246	arg1	presence					2313:2320	presence	2313:2320	presence of natural contaminants	2313:2344	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	5	35	theme	2.1-2.5	1347:1353	arg1	%					1342:1342	a CV%	1338:1342	a CV% of 2.1-2.5	1338:1353	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	5	35	theme	2.1-2.5	1347:1353	arg1	0.92-0.93					1324:1332	0.92-0.93	1324:1332	0.92-0.93	1324:1332	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	5	35	theme	2.1-2.5	1347:1353	arg1	2.1-2.5					1347:1353	2.1-2.5	1347:1353	2.1-2.5	1347:1353	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	4	36	theme	%	1091:1091	arg1	∼2.0-7.5					1038:1045	∼2.0-7.5	1038:1045	∼2.0-7.5	1038:1045	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	4	36	theme	%	1091:1091	arg1	variability					1068:1078	a polydispersity variability	1051:1078	a polydispersity variability of 0.7-2.7 %	1051:1091	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	1	37	theme	chicken	341:347	arg1	keel					349:352	chicken keel	341:352	chicken keel	341:352	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	38	theme	physicochemical	156:170	arg1	characteristics					172:186	physicochemical characteristics	156:186	physicochemical characteristics	156:186	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	10	39	theme	disaccharide	2287:2298	arg1	composition					2300:2310	disaccharide composition	2287:2310	disaccharide composition	2287:2310	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	2	40	theme	biological	646:655	arg1	contaminants					657:668	biological contaminants	646:668	biological contaminants	646:668	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	9	41	theme	origin	2086:2091	arg1	samples					2063:2069	CS samples	2060:2069	CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared	2060:2215	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	4	42	dep	weight	923:928	arg1	MWw					931:933	MWw	931:933	MWw of ∼21,500 Da	931:947	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	6	43	theme	hyaluronic	1477:1486	arg1	sulfate					1502:1508	keratan sulfate	1494:1508	keratan sulfate	1494:1508	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	43	theme	hyaluronic	1477:1486	arg1	acids					1554:1558	nucleic acids	1546:1558	nucleic acids	1546:1558	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	43	theme	hyaluronic	1477:1486	arg1	acid					1488:1491	hyaluronic acid	1477:1491	hyaluronic acid	1477:1491	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	43	theme	hyaluronic	1477:1486	arg1	sulfate					1520:1526	dermatan sulfate	1511:1526	dermatan sulfate	1511:1526	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	43	theme	hyaluronic	1477:1486	arg1	proteins					1564:1571	proteins	1564:1571	proteins	1564:1571	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	43	theme	hyaluronic	1477:1486	arg1	sulfate					1537:1543	heparan sulfate	1529:1543	heparan sulfate	1529:1543	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	4	44	theme	Da	946:947	arg1	MWw					931:933	MWw	931:933	MWw of ∼21,500 Da	931:947	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	0	45	theme	process	111:117	arg1	repeatability					79:91	repeatability	79:91	repeatability of the production process	79:117	Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process.					
33358299	0	45	theme	process	111:117	arg1	definition					11:20	Structural definition	0:20	Structural definition of terrestrial chondroitin sulfate of various origin	0:73	Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process.					
33358299	10	46	theme	particle	2350:2357	arg1	dimension					2359:2367	particle dimension	2350:2367	particle dimension	2350:2367	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	1	47	from	results	130:136	arg1	purity					192:197	purity	192:197	purity	192:197	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	47	from	results	130:136	arg1	structure					145:153	the structure	141:153	the structure	141:153	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	47	from	results	130:136	arg1	characteristics					172:186	physicochemical characteristics	156:186	physicochemical characteristics	156:186	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	6	48	dep	molecules	1466:1474	arg1	sulfate					1502:1508	keratan sulfate	1494:1508	keratan sulfate	1494:1508	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	48	dep	molecules	1466:1474	arg1	acids					1554:1558	nucleic acids	1546:1558	nucleic acids	1546:1558	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	48	dep	molecules	1466:1474	arg1	acid					1488:1491	hyaluronic acid	1477:1491	hyaluronic acid	1477:1491	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	48	dep	molecules	1466:1474	arg1	sulfate					1520:1526	dermatan sulfate	1511:1526	dermatan sulfate	1511:1526	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	48	dep	molecules	1466:1474	arg1	proteins					1564:1571	proteins	1564:1571	proteins	1564:1571	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	48	dep	molecules	1466:1474	arg1	sulfate					1537:1543	heparan sulfate	1529:1543	heparan sulfate	1529:1543	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	1	49	dep	bovine	310:315	arg1	trachea					329:335	trachea	329:335	trachea	329:335	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	5	50	theme	%	1342:1342	arg1	density					1313:1319	a overall charge density	1296:1319	a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5	1296:1353	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	9	51	theme	production	2121:2130	arg1	protocol					2132:2139	standardized production protocol	2108:2139	standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared	2108:2215	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	0	52	theme	Structural	0:9	arg1	definition					11:20	Structural definition	0:20	Structural definition of terrestrial chondroitin sulfate of various origin	0:73	Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process.					
33358299	1	53	theme	common	276:281	arg1	sources					294:300	three largely available and common biological sources	248:300	three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	248:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	53	theme	common	276:281	arg1	bones					354:358	bovine and porcine trachea and chicken keel bones	310:358	bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	310:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	4	54	dep	products	1015:1022	arg1	MWw					996:998	MWw	996:998	MWw of ∼35,900 Da	996:1012	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	2	55	theme	CS	425:426	arg1	CS					425:426	CS	425:426	CS produced by a manufacturer at industrial scale	425:473	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	2	55	theme	CS	425:426	arg1	lots					417:420	Many lots	412:420	Many lots of CS produced by a manufacturer at industrial scale	412:473	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	2	56	with	efficacy	703:710	arg1	structure					632:640	variable structure	623:640	variable structure	623:640	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	2	56	with	efficacy	703:710	arg1	contaminants					657:668	biological contaminants	646:668	biological contaminants	646:668	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	7	57	theme	optical	1719:1725	arg1	rotation					1727:1734	specific optical rotation	1710:1734	specific optical rotation	1710:1734	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	8	58	theme	reproducible	1914:1925	arg1	process					1941:1947	a standardized and highly reproducible manufacturing process	1888:1947	a standardized and highly reproducible manufacturing process	1888:1947	The observed parameters are structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process.					
33358299	2	59	theme	industrial	458:467	arg1	scale					469:473	industrial scale	458:473	industrial scale	458:473	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	9	60	theme	future	2150:2155	arg1	products					2192:2199	future pharmaceutical/nutraceutical final products	2150:2199	future pharmaceutical/nutraceutical final products	2150:2199	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	0	61	theme	chondroitin	37:47	arg1	sulfate					49:55	terrestrial chondroitin sulfate	25:55	terrestrial chondroitin sulfate of various origin	25:73	Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process.					
33358299	2	62	with	safety	716:721	arg1	structure					632:640	variable structure	623:640	variable structure	623:640	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	2	62	with	safety	716:721	arg1	contaminants					657:668	biological contaminants	646:668	biological contaminants	646:668	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	4	63	theme	Da	979:980	arg1	MWw					964:966	MWw	964:966	MWw of ∼26,000 Da	964:980	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	8	64	theme	observed	1777:1784	arg1	parameters					1786:1795	The observed parameters	1773:1795	The observed parameters	1773:1795	The observed parameters are structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process.					
33358299	8	64	theme	observed	1777:1784	arg1	signatures					1812:1821	structural signatures	1801:1821	structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process	1801:1947	The observed parameters are structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process.					
33358299	9	65	theme	final	2186:2190	arg1	products					2192:2199	future pharmaceutical/nutraceutical final products	2150:2199	future pharmaceutical/nutraceutical final products	2150:2199	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	7	66	theme	disaccharide	1684:1695	arg1	composition					1697:1707	disaccharide composition	1684:1707	disaccharide composition	1684:1707	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	7	67	theme	high	1635:1638	arg1	reproducibility					1640:1654	a high reproducibility	1633:1654	a high reproducibility of molecular weight values	1633:1681	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	7	68	theme	values	1676:1681	arg1	reproducibility					1640:1654	a high reproducibility	1633:1654	a high reproducibility of molecular weight values	1633:1681	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	7	68	theme	values	1676:1681	arg1	rotation					1727:1734	specific optical rotation	1710:1734	specific optical rotation	1710:1734	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	7	68	theme	values	1676:1681	arg1	dimension					1749:1757	particle dimension	1740:1757	particle dimension	1740:1757	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	7	68	theme	values	1676:1681	arg1	composition					1697:1707	disaccharide composition	1684:1707	disaccharide composition	1684:1707	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	8	69	dep	signatures	1812:1821	arg1	useful					1823:1828	useful	1823:1828	useful	1823:1828	The observed parameters are structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process.					
33358299	8	69	dep	signatures	1812:1821	arg1	obtained					1876:1883	obtained	1876:1883	obtained by a standardized and highly reproducible manufacturing process	1876:1947	The observed parameters are structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process.					
33358299	10	70	theme	contaminants	2333:2344	arg1	presence					2313:2320	presence	2313:2320	presence of natural contaminants	2313:2344	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	10	70	theme	contaminants	2333:2344	arg1	weight					2279:2284	molecular weight	2269:2284	molecular weight	2269:2284	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	10	70	theme	contaminants	2333:2344	arg1	composition					2300:2310	disaccharide composition	2287:2310	disaccharide composition	2287:2310	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	10	70	theme	contaminants	2333:2344	arg1	dimension					2359:2367	particle dimension	2350:2367	particle dimension	2350:2367	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	9	71	theme	compositional	1954:1966	arg1	profile					1968:1974	The compositional profile	1950:1974	The compositional profile determined from this study	1950:2001	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	9	72	from	range	2038:2042	arg1	samples					2063:2069	CS samples	2060:2069	CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared	2060:2215	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	5	73	theme	sulfate	1116:1122	arg1	groups					1124:1129	the sulfate groups	1112:1129	the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio)	1112:1200	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	4	74	theme	Da	1011:1012	arg1	MWw					996:998	MWw	996:998	MWw of ∼35,900 Da	996:1012	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	9	75	from	norm	2029:2032	arg1	samples					2063:2069	CS samples	2060:2069	CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared	2060:2215	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	1	76	theme	available	262:270	arg1	sources					294:300	three largely available and common biological sources	248:300	three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	248:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	76	theme	available	262:270	arg1	bones					354:358	bovine and porcine trachea and chicken keel bones	310:358	bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	310:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	2	77	theme	extractive	569:578	arg1	procedures					580:589	not controlled extractive procedures	554:589	not controlled extractive procedures	554:589	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	9	78	dep	norm	2029:2032	arg1	the					2025:2027	the	2025:2027	the	2025:2027	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	4	79	theme	variability	1068:1078	arg1	∼2.0-7.5					1038:1045	∼2.0-7.5	1038:1045	∼2.0-7.5	1038:1045	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	4	79	theme	variability	1068:1078	arg1	%					1033:1033	a CV%	1029:1033	a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %	1029:1091	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	4	79	theme	variability	1068:1078	arg1	variability					1068:1078	a polydispersity variability	1051:1078	a polydispersity variability of 0.7-2.7 %	1051:1091	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	9	80	from	samples	2063:2069	arg1	range					2038:2042	range	2038:2042	range	2038:2042	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	9	80	from	samples	2063:2069	arg1	norm					2029:2032	norm	2029:2032	norm	2029:2032	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	2	81	theme	final	603:607	arg1	products					609:616	final products	603:616	final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety	603:721	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	5	82	theme	charge	1306:1311	arg1	density					1313:1319	a overall charge density	1296:1319	a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5	1296:1353	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	1	83	theme	chondroitin	202:212	arg1	sulfate					214:220	chondroitin sulfate	202:220	chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	202:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	83	theme	chondroitin	202:212	arg1	CS					223:224	CS	223:224	CS	223:224	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	4	84	theme	lower	907:911	arg1	weight					923:928	a lower molecular weight	905:928	a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products	905:1022	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	4	85	theme	∼2.0-7.5	1038:1045	arg1	∼2.0-7.5					1038:1045	∼2.0-7.5	1038:1045	∼2.0-7.5	1038:1045	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	4	85	theme	∼2.0-7.5	1038:1045	arg1	%					1033:1033	a CV%	1029:1033	a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %	1029:1091	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	4	85	theme	∼2.0-7.5	1038:1045	arg1	variability					1068:1078	a polydispersity variability	1051:1078	a polydispersity variability of 0.7-2.7 %	1051:1091	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	3	86	theme	source	746:751	arg1	tissues					760:766	standardized source animal tissues	733:766	standardized source animal tissues	733:766	By using standardized source animal tissues and manufacturing procedure, highly pure CS (∼92 %) products with constant structure and characteristics were obtained.					
33358299	1	87	theme	structural	389:398	arg1	signatures					400:409	their structural signatures	383:409	their structural signatures	383:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	6	88	theme	co-purified	1444:1454	arg1	molecules					1466:1474	other co-purified bio(macro)molecules	1438:1474	other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins)	1438:1572	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	88	theme	co-purified	1444:1454	arg1	sodium					1587:1592	sodium	1587:1592	sodium	1587:1592	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	88	theme	co-purified	1444:1454	arg1	calcium					1575:1581	calcium	1575:1581	calcium	1575:1581	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	4	89	theme	Bovine	888:893	arg1	CS					895:896	Bovine CS	888:896	Bovine CS	888:896	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	6	90	theme	dermatan	1511:1518	arg1	acid					1488:1491	hyaluronic acid	1477:1491	hyaluronic acid	1477:1491	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	90	theme	dermatan	1511:1518	arg1	sulfate					1520:1526	dermatan sulfate	1511:1526	dermatan sulfate	1511:1526	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	2	91	theme	clinical	694:701	arg1	efficacy					703:710	constant clinical efficacy	685:710	constant clinical efficacy	685:710	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	10	92	theme	high	2418:2421	arg1	quality					2423:2429	high quality	2418:2429	high quality	2418:2429	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	9	93	from	variation	2047:2055	arg1	samples					2063:2069	CS samples	2060:2069	CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared	2060:2215	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	3	94	with	products	820:827	arg1	structure					843:851	constant structure	834:851	constant structure	834:851	By using standardized source animal tissues and manufacturing procedure, highly pure CS (∼92 %) products with constant structure and characteristics were obtained.					
33358299	3	94	with	products	820:827	arg1	characteristics					857:871	characteristics	857:871	characteristics	857:871	By using standardized source animal tissues and manufacturing procedure, highly pure CS (∼92 %) products with constant structure and characteristics were obtained.					
33358299	2	95	theme	process	543:549	arg1	reproducibility					520:534	the reproducibility	516:534	the reproducibility of the process	516:549	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	10	96	theme	CS	2412:2413	arg1	basis					2403:2407	the basis	2399:2407	the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents	2399:2491	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	6	97	theme	constant	1418:1425	arg1	content					1427:1433	a very low and constant content	1403:1433	a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium,	1403:1593	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	3	98	theme	constant	834:841	arg1	structure					843:851	constant structure	834:851	constant structure	834:851	By using standardized source animal tissues and manufacturing procedure, highly pure CS (∼92 %) products with constant structure and characteristics were obtained.					
33358299	3	99	theme	pure	804:807	arg1	CS					809:810	highly pure CS	797:810	highly pure CS (∼92 %) products with constant structure and characteristics	797:871	By using standardized source animal tissues and manufacturing procedure, highly pure CS (∼92 %) products with constant structure and characteristics were obtained.					
33358299	3	99	theme	pure	804:807	arg1	%					817:817	∼92 %	813:817	∼92 %	813:817	By using standardized source animal tissues and manufacturing procedure, highly pure CS (∼92 %) products with constant structure and characteristics were obtained.					
33358299	8	100	theme	structural	1801:1810	arg1	parameters					1786:1795	The observed parameters	1773:1795	The observed parameters	1773:1795	The observed parameters are structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process.					
33358299	8	100	theme	structural	1801:1810	arg1	signatures					1812:1821	structural signatures	1801:1821	structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process	1801:1947	The observed parameters are structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process.					
33358299	6	101	theme	low	1410:1412	arg1	content					1427:1433	a very low and constant content	1403:1433	a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium,	1403:1593	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	5	102	theme	N-acetyl-galactosamine	1167:1188	arg1	position					1147:1154	position 4 and 6	1147:1162	position 4 and 6 of N-acetyl-galactosamine (4/6 ratio)	1147:1200	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	9	103	theme	terrestrial	2074:2084	arg1	origin					2086:2091	terrestrial origin	2074:2091	terrestrial origin	2074:2091	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	0	104	theme	production	100:109	arg1	process					111:117	the production process	96:117	the production process	96:117	Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process.					
33358299	1	105	theme	keel	349:352	arg1	bones					354:358	bovine and porcine trachea and chicken keel bones	310:358	bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	310:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	7	106	theme	particle	1740:1747	arg1	dimension					1749:1757	particle dimension	1740:1757	particle dimension	1740:1757	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	8	107	theme	standardized	1890:1901	arg1	process					1941:1947	a standardized and highly reproducible manufacturing process	1888:1947	a standardized and highly reproducible manufacturing process	1888:1947	The observed parameters are structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process.					
33358299	10	108	theme	molecular	2269:2277	arg1	weight					2279:2284	molecular weight	2269:2284	molecular weight	2269:2284	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	6	109	theme	nucleic	1546:1552	arg1	acids					1554:1558	nucleic acids	1546:1558	nucleic acids	1546:1558	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	109	theme	nucleic	1546:1552	arg1	acid					1488:1491	hyaluronic acid	1477:1491	hyaluronic acid	1477:1491	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	5	110	theme	CV	1340:1341	arg1	%					1342:1342	a CV%	1338:1342	a CV% of 2.1-2.5	1338:1353	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	5	110	theme	CV	1340:1341	arg1	2.1-2.5					1347:1353	2.1-2.5	1347:1353	2.1-2.5	1347:1353	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	10	111	theme	active	2479:2484	arg1	agents					2486:2491	pharmaceutical/nutraceutical active agents	2450:2491	pharmaceutical/nutraceutical active agents	2450:2491	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	9	112	theme	standardized	2108:2119	arg1	protocol					2132:2139	standardized production protocol	2108:2139	standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared	2108:2215	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	6	113	theme	versican	1614:1621	arg1	absence					1603:1609	the absence	1599:1609	the absence of versican	1599:1621	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	6	113	theme	versican	1614:1621	arg1	presence					1391:1398	the presence	1387:1398	the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium,	1387:1593	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	2	114	from	scale	469:473	arg1	manufacturer					442:453	a manufacturer	440:453	a manufacturer at industrial scale	440:473	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	0	115	theme	terrestrial	25:35	arg1	sulfate					49:55	terrestrial chondroitin sulfate	25:55	terrestrial chondroitin sulfate of various origin	25:73	Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process.					
33358299	1	116	theme	biological	283:292	arg1	sources					294:300	three largely available and common biological sources	248:300	three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	248:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	116	theme	biological	283:292	arg1	bones					354:358	bovine and porcine trachea and chicken keel bones	310:358	bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	310:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	7	117	theme	specific	1710:1717	arg1	rotation					1727:1734	specific optical rotation	1710:1734	specific optical rotation	1710:1734	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	8	118	theme	manufacturing	1927:1939	arg1	process					1941:1947	a standardized and highly reproducible manufacturing process	1888:1947	a standardized and highly reproducible manufacturing process	1888:1947	The observed parameters are structural signatures useful to specifically identify the origin of CS and obtained by a standardized and highly reproducible manufacturing process.					
33358299	4	119	theme	chicken	987:993	arg1	products					1015:1022	porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products	955:1022	products	1015:1022	Bovine CS showed a lower molecular weight (MWw of ∼21,500 Da) than porcine (MWw of ∼26,000 Da) and chicken (MWw of ∼35,900 Da) products with a CV% of ∼2.0-7.5 and a polydispersity variability of 0.7-2.7 %.					
33358299	5	120	theme	bovine	1216:1221	arg1	CS					1223:1224	bovine CS	1216:1224	bovine CS	1216:1224	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	2	121	theme	Many	412:415	arg1	CS					425:426	CS	425:426	CS produced by a manufacturer at industrial scale	425:473	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	2	121	theme	Many	412:415	arg1	lots					417:420	Many lots	412:420	Many lots of CS produced by a manufacturer at industrial scale	412:473	Many lots of CS produced by a manufacturer at industrial scale were characterized with a view to assess the reproducibility of the process as not controlled extractive procedures may produce final products with variable structure and biological contaminants as well as not constant clinical efficacy and safety.					
33358299	0	122	theme	sulfate	49:55	arg1	repeatability					79:91	repeatability	79:91	repeatability of the production process	79:117	Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process.					
33358299	0	122	theme	sulfate	49:55	arg1	definition					11:20	Structural definition	0:20	Structural definition of terrestrial chondroitin sulfate of various origin	0:73	Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process.					
33358299	9	123	theme	pharmaceutical/nutraceutical	2157:2184	arg1	products					2192:2199	future pharmaceutical/nutraceutical final products	2150:2199	future pharmaceutical/nutraceutical final products	2150:2199	The compositional profile determined from this study provides a measure of the norm and range of variation in CS samples of terrestrial origin produced under standardized production protocol to which future pharmaceutical/nutraceutical final products can be compared.					
33358299	0	124	theme	various	60:66	arg1	origin					68:73	various origin	60:73	various origin	60:73	Structural definition of terrestrial chondroitin sulfate of various origin and repeatability of the production process.					
33358299	1	125	theme	bovine	310:315	arg1	bones					354:358	bovine and porcine trachea and chicken keel bones	310:358	bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	310:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	7	126	theme	molecular	1659:1667	arg1	values					1676:1681	molecular weight values	1659:1681	molecular weight values	1659:1681	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	10	127	theme	natural	2325:2331	arg1	contaminants					2333:2344	natural contaminants	2325:2344	natural contaminants	2325:2344	Moreover, the physicochemical properties including molecular weight, disaccharide composition, presence of natural contaminants and particle dimension were characterized to provide the basis of CS of high quality for application as pharmaceutical/nutraceutical active agents.					
33358299	5	128	theme	chicken	1275:1281	arg1	samples					1283:1289	chicken samples	1275:1289	chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5	1275:1353	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	6	129	theme	final	1360:1364	arg1	products					1366:1373	The final products	1356:1373	The final products	1356:1373	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	1	130	theme	porcine	321:327	arg1	bones					354:358	bovine and porcine trachea and chicken keel bones	310:358	bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures	310:409	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	7	131	theme	weight	1669:1674	arg1	values					1676:1681	molecular weight values	1659:1681	molecular weight values	1659:1681	Finally, a high reproducibility of molecular weight values, disaccharide composition, specific optical rotation and particle dimension was observed.					
33358299	6	132	theme	molecules	1466:1474	arg1	content					1427:1433	a very low and constant content	1403:1433	a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium,	1403:1593	The final products also showed the presence of a very low and constant content of other co-purified bio(macro)molecules (hyaluronic acid, keratan sulfate, dermatan sulfate, heparan sulfate, nucleic acids and proteins), calcium and sodium, and the absence of versican.					
33358299	5	133	theme	3.60	1244:1247	arg1	value					1235:1239	a value	1233:1239	a value of 3.60 for porcine	1233:1259	The ratio between the sulfate groups main located in position 4 and 6 of N-acetyl-galactosamine (4/6 ratio) was ∼1.70 for bovine CS versus a value of 3.60 for porcine and ∼2.70 for chicken samples with a overall charge density of 0.92-0.93 and a CV% of 2.1-2.5.					
33358299	1	134	theme	samples	227:233	arg1	purity					192:197	purity	192:197	purity	192:197	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	134	theme	samples	227:233	arg1	structure					145:153	the structure	141:153	the structure	141:153	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
33358299	1	134	theme	samples	227:233	arg1	characteristics					172:186	physicochemical characteristics	156:186	physicochemical characteristics	156:186	We report results on the structure, physicochemical characteristics and purity of chondroitin sulfate (CS) samples derived from three largely available and common biological sources such as bovine and porcine trachea and chicken keel bones with the aim to define their structural signatures.					
32943624	6	0	theme	elongation	1222:1231	arg1	increase					1206:1213	a dramatic increase	1195:1213	a dramatic increase of the elongation at break under tension	1195:1254	The wood hemicelluloses exhibit distinct biomechanical contributions, with glucomannans increasing the elastic modulus in compression, and xylans contributing to a dramatic increase of the elongation at break under tension.					
32943624	8	1	theme	lignocellulosic	1779:1793	arg1	materials					1795:1803	lignocellulosic materials	1779:1803	lignocellulosic materials with controlled assembly and tailored mechanical properties	1779:1863	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	1	2	theme	heterogeneous	124:136	arg1	polysaccharides					138:152	heterogeneous polysaccharides	124:152	heterogeneous polysaccharides with complex molecular structures	124:186	Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass.					
32943624	1	3	theme	fundamental	202:212	arg1	component					214:222	a fundamental component	200:222	a fundamental component of lignocellulosic biomass	200:249	Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass.					
32943624	5	4	dep	phase	872:876	arg1	both					859:862	both	859:862	both	859:862	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	7	5	theme	hemicellulose-coated	1533:1552	arg1	fibrils					1554:1560	hemicellulose-coated fibrils	1533:1560	hemicellulose-coated fibrils	1533:1560	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	2	6	theme	cell	353:356	arg1	walls					358:362	secondary plant cell walls	337:362	secondary plant cell walls	337:362	However, the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls remains elusive.					
32943624	5	7	theme	multiscale	971:980	arg1	architecture					982:993	the multiscale architecture	967:993	the multiscale architecture of the bacterial cellulose hydrogels	967:1030	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	2	8	theme	secondary	337:345	arg1	walls					358:362	secondary plant cell walls	337:362	secondary plant cell walls	337:362	However, the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls remains elusive.					
32943624	1	9	theme	biomass	243:249	arg1	component					214:222	a fundamental component	200:222	a fundamental component of lignocellulosic biomass	200:249	Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass.					
32943624	6	10	theme	biomechanical	1074:1086	arg1	contributions					1088:1100	distinct biomechanical contributions	1065:1100	distinct biomechanical contributions	1065:1100	The wood hemicelluloses exhibit distinct biomechanical contributions, with glucomannans increasing the elastic modulus in compression, and xylans contributing to a dramatic increase of the elongation at break under tension.					
32943624	7	11	theme	hydrogels	1487:1495	arg1	structure					1414:1422	the distinct molecular structure	1391:1422	the distinct molecular structure of wood xylans and mannans	1391:1449	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	7	11	theme	hydrogels	1487:1495	arg1	effects					1517:1523	the aggregative effects	1501:1523	the aggregative effects amongst hemicellulose-coated fibrils	1501:1560	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	7	11	theme	hydrogels	1487:1495	arg1	architecture					1467:1478	the multiphase architecture	1452:1478	the multiphase architecture of the hydrogels	1452:1495	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	8	12	contain	has	1739:1741	arg2	significance					1743:1754	significance	1743:1754	significance	1743:1754	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	8	12	contain	has	1739:1741	arg1	study					1567:1571	Our study	1563:1571	Our study	1563:1571	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	4	13	theme	prior	795:799	arg1	walls					789:793	secondary plant cell walls	768:793	secondary plant cell walls prior to lignification	768:816	These composite hydrogels can be therefore envisioned as models of secondary plant cell walls prior to lignification.					
32943624	5	14	theme	hydrogels	1022:1030	arg1	architecture					982:993	the multiscale architecture	967:993	the multiscale architecture of the bacterial cellulose hydrogels	967:1030	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	6	15	theme	wood	1037:1040	arg1	hemicelluloses					1042:1055	The wood hemicelluloses	1033:1055	The wood hemicelluloses	1033:1055	The wood hemicelluloses exhibit distinct biomechanical contributions, with glucomannans increasing the elastic modulus in compression, and xylans contributing to a dramatic increase of the elongation at break under tension.					
32943624	7	16	with	interactions	1340:1351	arg1	cellulose					1358:1366	cellulose	1358:1366	cellulose	1358:1366	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	3	17	theme	cellulose	682:690	arg1	bundles					692:698	the cellulose bundles	678:698	the cellulose bundles	678:698	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	2	18	theme	type	300:303	arg1	contribution					265:276	the contribution	261:276	the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls	261:362	However, the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls remains elusive.					
32943624	1	19	theme	complex	159:165	arg1	structures					177:186	complex molecular structures	159:186	complex molecular structures	159:186	Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass.					
32943624	8	20	theme	wood	1624:1627	arg1	xylans					1629:1634	wood xylans	1624:1634	wood xylans	1624:1634	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	7	21	theme	multiphase	1456:1465	arg1	architecture					1467:1478	the multiphase architecture	1452:1478	the multiphase architecture of the hydrogels	1452:1495	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	4	22	theme	secondary	768:776	arg1	walls					789:793	secondary plant cell walls	768:793	secondary plant cell walls prior to lignification	768:816	These composite hydrogels can be therefore envisioned as models of secondary plant cell walls prior to lignification.					
32943624	0	23	theme	fibrillar	76:84	arg1	networks					86:93	cellulose fibrillar networks	66:93	cellulose fibrillar networks	66:93	Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks.					
32943624	3	24	theme	bundles	692:698	arg1	morphology					642:651	the morphology	638:651	the morphology	638:651	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	3	24	theme	bundles	692:698	arg1	crystallinity					661:673	the crystallinity	657:673	the crystallinity of the cellulose bundles	657:698	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	4	25	theme	cell	784:787	arg1	walls					789:793	secondary plant cell walls	768:793	secondary plant cell walls prior to lignification	768:816	These composite hydrogels can be therefore envisioned as models of secondary plant cell walls prior to lignification.					
32943624	5	26	theme	rigid	866:870	arg1	phase					872:876	a rigid phase	864:876	a rigid phase having close interactions with cellulose	864:917	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	8	27	theme	xylans	1629:1634	arg1	roles					1615:1619	the specific roles	1602:1619	the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression	1602:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	5	28	theme	bacterial	1002:1010	arg1	hydrogels					1022:1030	the bacterial cellulose hydrogels	998:1030	the bacterial cellulose hydrogels	998:1030	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	3	29	dep	extracted	441:449	arg1	glucomannans					491:502	glucomannans	491:502	glucomannans	491:502	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	3	29	dep	extracted	441:449	arg1	xylans					480:485	xylans	480:485	xylans	480:485	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	7	30	theme	direct	1333:1338	arg1	interactions					1340:1351	their direct interactions	1327:1351	their direct interactions with cellulose	1327:1366	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	3	31	from	combinations	425:436	arg1	species					532:538	softwood and hardwood species	510:538	softwood and hardwood species	510:538	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	0	32	theme	cellulose	66:74	arg1	networks					86:93	cellulose fibrillar networks	66:93	cellulose fibrillar networks	66:93	Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks.					
32943624	8	33	theme	biomechanical	1660:1672	arg1	integrity					1674:1682	the biomechanical integrity	1656:1682	the biomechanical integrity of secondary cell walls in tension and compression	1656:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	8	34	from	roles	1615:1619	arg1	integrity					1674:1682	the biomechanical integrity	1656:1682	the biomechanical integrity of secondary cell walls in tension and compression	1656:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	3	35	theme	extracted	441:449	arg1	combinations					425:436	different combinations	415:436	different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks	415:567	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	0	36	theme	Wood	0:3	arg1	hemicelluloses					5:18	Wood hemicelluloses	0:18	Wood hemicelluloses	0:18	Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks.					
32943624	1	37	with	polysaccharides	138:152	arg1	structures					177:186	complex molecular structures	159:186	complex molecular structures	159:186	Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass.					
32943624	7	38	theme	mannans	1443:1449	arg1	structure					1414:1422	the distinct molecular structure	1391:1422	the distinct molecular structure of wood xylans and mannans	1391:1449	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	7	38	theme	mannans	1443:1449	arg1	effects					1517:1523	the aggregative effects	1501:1523	the aggregative effects amongst hemicellulose-coated fibrils	1501:1560	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	7	38	theme	mannans	1443:1449	arg1	architecture					1467:1478	the multiphase architecture	1452:1478	the multiphase architecture of the hydrogels	1452:1495	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	8	39	theme	cell	1697:1700	arg1	walls					1702:1706	secondary cell walls	1687:1706	secondary cell walls in tension and compression	1687:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	3	40	theme	hardwood	523:530	arg1	species					532:538	softwood and hardwood species	510:538	softwood and hardwood species	510:538	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	3	41	theme	bacterial	604:612	arg1	model					614:618	a bacterial model	602:618	a bacterial model	602:618	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	7	42	theme	xylans	1432:1437	arg1	structure					1414:1422	the distinct molecular structure	1391:1422	the distinct molecular structure of wood xylans and mannans	1391:1449	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	7	42	theme	xylans	1432:1437	arg1	effects					1517:1523	the aggregative effects	1501:1523	the aggregative effects amongst hemicellulose-coated fibrils	1501:1560	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	7	42	theme	xylans	1432:1437	arg1	architecture					1467:1478	the multiphase architecture	1452:1478	the multiphase architecture of the hydrogels	1452:1495	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	3	43	theme	softwood	510:517	arg1	species					532:538	softwood and hardwood species	510:538	softwood and hardwood species	510:538	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	0	44	theme	biomechanical	35:47	arg1	contributions					49:61	distinct biomechanical contributions	26:61	distinct biomechanical contributions to cellulose fibrillar networks	26:93	Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks.					
32943624	3	45	theme	purified	455:462	arg1	hemicelluloses					464:477	purified hemicelluloses	455:477	purified hemicelluloses	455:477	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	7	46	theme	molecular	1404:1412	arg1	structure					1414:1422	the distinct molecular structure	1391:1422	the distinct molecular structure of wood xylans and mannans	1391:1449	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	3	47	from	species	532:538	arg1	combinations					425:436	different combinations	415:436	different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks	415:567	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	8	48	theme	controlled	1810:1819	arg1	assembly					1821:1828	controlled assembly	1810:1828	controlled assembly	1810:1828	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	7	49	theme	diverging	1263:1271	arg1	effects					1273:1279	These diverging effects	1257:1279	These diverging effects	1257:1279	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	8	50	from	tension	1711:1717	arg1	integrity					1674:1682	the biomechanical integrity	1656:1682	the biomechanical integrity of secondary cell walls in tension and compression	1656:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	3	51	theme	cellulose	576:584	arg1	biosynthesis					586:597	cellulose biosynthesis	576:597	cellulose biosynthesis in a bacterial model	576:618	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	8	52	theme	mechanical	1843:1852	arg1	properties					1854:1863	tailored mechanical properties	1834:1863	tailored mechanical properties	1834:1863	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	5	53	dep	together	920:927	arg1	with					929:932	with	929:932	with	929:932	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	8	54	from	walls	1702:1706	arg1	compression					1723:1733	compression	1723:1733	compression	1723:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	8	54	from	walls	1702:1706	arg1	tension					1711:1717	tension	1711:1717	tension	1711:1717	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	3	55	from	biosynthesis	586:597	arg1	model					614:618	a bacterial model	602:618	a bacterial model	602:618	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	8	56	theme	materials	1795:1803	arg1	development					1764:1774	the development	1760:1774	the development of lignocellulosic materials with controlled assembly and tailored mechanical properties	1760:1863	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	2	57	theme	mechanical	312:321	arg1	properties					323:332	the mechanical properties	308:332	the mechanical properties of secondary plant cell walls	308:362	However, the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls remains elusive.					
32943624	8	58	from	compression	1723:1733	arg1	integrity					1674:1682	the biomechanical integrity	1656:1682	the biomechanical integrity of secondary cell walls in tension and compression	1656:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	2	59	theme	walls	358:362	arg1	properties					323:332	the mechanical properties	308:332	the mechanical properties of secondary plant cell walls	308:362	However, the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls remains elusive.					
32943624	2	60	theme	plant	347:351	arg1	walls					358:362	secondary plant cell walls	337:362	secondary plant cell walls	337:362	However, the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls remains elusive.					
32943624	1	61	theme	lignocellulosic	227:241	arg1	biomass					243:249	lignocellulosic biomass	227:249	lignocellulosic biomass	227:249	Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass.					
32943624	8	62	with	materials	1795:1803	arg1	properties					1854:1863	tailored mechanical properties	1834:1863	tailored mechanical properties	1834:1863	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	8	62	with	materials	1795:1803	arg1	assembly					1821:1828	controlled assembly	1810:1828	controlled assembly	1810:1828	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	4	63	theme	composite	707:715	arg1	hydrogels					717:725	These composite hydrogels	701:725	These composite hydrogels	701:725	These composite hydrogels can be therefore envisioned as models of secondary plant cell walls prior to lignification.					
32943624	4	63	theme	composite	707:715	arg1	models					758:763	models	758:763	models of secondary plant cell walls prior to lignification	758:816	These composite hydrogels can be therefore envisioned as models of secondary plant cell walls prior to lignification.					
32943624	5	64	theme	flexible	936:943	arg1	phase					945:949	a flexible phase	934:949	a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels	934:1030	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	6	65	theme	distinct	1065:1072	arg1	contributions					1088:1100	distinct biomechanical contributions	1065:1100	distinct biomechanical contributions	1065:1100	The wood hemicelluloses exhibit distinct biomechanical contributions, with glucomannans increasing the elastic modulus in compression, and xylans contributing to a dramatic increase of the elongation at break under tension.					
32943624	8	66	theme	specific	1606:1613	arg1	roles					1615:1619	the specific roles	1602:1619	the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression	1602:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	7	67	theme	aggregative	1505:1515	arg1	effects					1517:1523	the aggregative effects	1501:1523	the aggregative effects amongst hemicellulose-coated fibrils	1501:1560	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	5	68	theme	cellulose	1012:1020	arg1	hydrogels					1022:1030	the bacterial cellulose hydrogels	998:1030	the bacterial cellulose hydrogels	998:1030	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	4	69	theme	walls	789:793	arg1	hydrogels					717:725	These composite hydrogels	701:725	These composite hydrogels	701:725	These composite hydrogels can be therefore envisioned as models of secondary plant cell walls prior to lignification.					
32943624	4	69	theme	walls	789:793	arg1	models					758:763	models	758:763	models of secondary plant cell walls prior to lignification	758:816	These composite hydrogels can be therefore envisioned as models of secondary plant cell walls prior to lignification.					
32943624	5	70	contain	having	878:883	arg1	phase					872:876	a rigid phase	864:876	a rigid phase having close interactions with cellulose	864:917	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	5	70	contain	having	878:883	arg2	interactions					891:902	close interactions	885:902	close interactions	885:902	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	2	71	theme	hemicellulose	286:298	arg1	type					300:303	each hemicellulose type	281:303	each hemicellulose type	281:303	However, the contribution of each hemicellulose type to the mechanical properties of secondary plant cell walls remains elusive.					
32943624	1	72	theme	molecular	167:175	arg1	structures					177:186	complex molecular structures	159:186	complex molecular structures	159:186	Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass.					
32943624	1	73	theme	polysaccharides	138:152	arg1	Hemicelluloses					96:109	Hemicelluloses	96:109	Hemicelluloses	96:109	Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass.					
32943624	1	73	theme	polysaccharides	138:152	arg1	family					114:119	a family	112:119	a family of heterogeneous polysaccharides with complex molecular structures	112:186	Hemicelluloses, a family of heterogeneous polysaccharides with complex molecular structures, constitute a fundamental component of lignocellulosic biomass.					
32943624	8	74	theme	glucomannans	1640:1651	arg1	roles					1615:1619	the specific roles	1602:1619	the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression	1602:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	7	75	theme	interactions	1340:1351	arg1	nature					1317:1322	the nature	1313:1322	the nature of their direct interactions with cellulose	1313:1366	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	6	76	theme	elastic	1136:1142	arg1	modulus					1144:1150	the elastic modulus	1132:1150	the elastic modulus in compression	1132:1165	The wood hemicelluloses exhibit distinct biomechanical contributions, with glucomannans increasing the elastic modulus in compression, and xylans contributing to a dramatic increase of the elongation at break under tension.					
32943624	6	77	from	modulus	1144:1150	arg1	compression					1155:1165	compression	1155:1165	compression	1155:1165	The wood hemicelluloses exhibit distinct biomechanical contributions, with glucomannans increasing the elastic modulus in compression, and xylans contributing to a dramatic increase of the elongation at break under tension.					
32943624	4	78	theme	plant	778:782	arg1	walls					789:793	secondary plant cell walls	768:793	secondary plant cell walls prior to lignification	768:816	These composite hydrogels can be therefore envisioned as models of secondary plant cell walls prior to lignification.					
32943624	3	79	theme	different	415:423	arg1	combinations					425:436	different combinations	415:436	different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks	415:567	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	5	80	theme	incorporated	823:834	arg1	hemicelluloses					836:849	The incorporated hemicelluloses	819:849	The incorporated hemicelluloses	819:849	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	8	81	theme	secondary	1687:1695	arg1	walls					1702:1706	secondary cell walls	1687:1706	secondary cell walls in tension and compression	1687:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	6	82	theme	dramatic	1197:1204	arg1	increase					1206:1213	a dramatic increase	1195:1213	a dramatic increase of the elongation at break under tension	1195:1254	The wood hemicelluloses exhibit distinct biomechanical contributions, with glucomannans increasing the elastic modulus in compression, and xylans contributing to a dramatic increase of the elongation at break under tension.					
32943624	8	83	theme	walls	1702:1706	arg1	integrity					1674:1682	the biomechanical integrity	1656:1682	the biomechanical integrity of secondary cell walls in tension and compression	1656:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	0	84	theme	distinct	26:33	arg1	contributions					49:61	distinct biomechanical contributions	26:61	distinct biomechanical contributions to cellulose fibrillar networks	26:93	Wood hemicelluloses exert distinct biomechanical contributions to cellulose fibrillar networks.					
32943624	7	85	theme	wood	1427:1430	arg1	xylans					1432:1437	wood xylans	1427:1437	wood xylans	1427:1437	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	5	86	theme	close	885:889	arg1	interactions					891:902	close interactions	885:902	close interactions	885:902	The incorporated hemicelluloses exhibit both a rigid phase having close interactions with cellulose, together with a flexible phase contributing to the multiscale architecture of the bacterial cellulose hydrogels.					
32943624	7	87	theme	distinct	1395:1402	arg1	structure					1414:1422	the distinct molecular structure	1391:1422	the distinct molecular structure of wood xylans and mannans	1391:1449	These diverging effects cannot be explained solely from the nature of their direct interactions with cellulose, but can be related to the distinct molecular structure of wood xylans and mannans, the multiphase architecture of the hydrogels and the aggregative effects amongst hemicellulose-coated fibrils.					
32943624	3	88	theme	hemicelluloses	464:477	arg1	combinations					425:436	different combinations	415:436	different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks	415:567	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	8	89	from	integrity	1674:1682	arg1	compression					1723:1733	compression	1723:1733	compression	1723:1733	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	8	89	from	integrity	1674:1682	arg1	tension					1711:1717	tension	1711:1717	tension	1711:1717	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
32943624	3	90	theme	self-assembled	545:558	arg1	networks					560:567	self-assembled networks	545:567	self-assembled networks	545:567	Here we homogeneously incorporate different combinations of extracted and purified hemicelluloses (xylans and glucomannans) from softwood and hardwood species into self-assembled networks during cellulose biosynthesis in a bacterial model, without altering the morphology and the crystallinity of the cellulose bundles.					
32943624	8	91	theme	tailored	1834:1841	arg1	properties					1854:1863	tailored mechanical properties	1834:1863	tailored mechanical properties	1834:1863	Our study contributes to understanding the specific roles of wood xylans and glucomannans in the biomechanical integrity of secondary cell walls in tension and compression and has significance for the development of lignocellulosic materials with controlled assembly and tailored mechanical properties.					
34279501	0	0	theme	Preservation	97:108	arg1	Performances					110:121	their Egg Preservation Performances	87:121	their Egg Preservation Performances	87:121	Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances.					
34279501	1	1	theme	minerals	166:173	arg1	source					146:151	an excellent source	133:151	an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world	133:256	Eggs are an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world.					
34279501	1	1	theme	minerals	166:173	arg1	Eggs					124:127	Eggs	124:127	Eggs	124:127	Eggs are an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world.					
34279501	0	2	theme	Egg	93:95	arg1	Performances					110:121	their Egg Preservation Performances	87:121	their Egg Preservation Performances	87:121	Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances.					
34279501	8	3	theme	coating	1495:1501	arg1	precursor					1503:1511	the coating precursor	1491:1511	the coating precursor	1491:1511	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	8	4	theme	coating	1411:1417	arg1	materials					1419:1427	novel coating materials	1405:1427	novel coating materials	1405:1427	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	7	5	theme	chitosan	1227:1234	arg1	coating					1236:1242	chitosan coating	1227:1242	chitosan coating	1227:1242	The performances of chitosan coating, however, are presently limited by the existing particle species and concentration, which requires optimization in future studies.					
34279501	8	6	theme	preservation	1662:1673	arg1	field					1648:1652	the field	1644:1652	the field of food preservation	1644:1673	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	5	7	theme	eggshell	1036:1043	arg1	morphologies					1045:1056	eggshell morphologies	1036:1056	eggshell morphologies of the samples	1036:1071	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	0	8	from	Functionalization	0:16	arg1	Performances					110:121	their Egg Preservation Performances	87:121	their Egg Preservation Performances	87:121	Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances.					
34279501	0	8	from	Functionalization	0:16	arg1	Coatings					74:81	Chitosan-based Coatings	59:81	Chitosan-based Coatings	59:81	Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances.					
34279501	4	9	theme	particles	857:865	arg1	structure					819:827	structure	819:827	structure	819:827	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	4	9	theme	particles	857:865	arg1	morphology					875:884	the morphology	871:884	the morphology of coated eggshells	871:904	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	4	9	theme	particles	857:865	arg1	morphology					833:842	morphology	833:842	morphology	833:842	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	5	10	theme	Haugh	1009:1013	arg1	unit					1015:1018	Haugh unit	1009:1018	Haugh unit	1009:1018	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	3	11	theme	chitosan-based	502:515	arg1	materials					525:533	the existing chitosan-based coating materials	489:533	the existing chitosan-based coating materials	489:533	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	8	12	theme	food	1657:1660	arg1	preservation					1662:1673	food preservation	1657:1673	food preservation	1657:1673	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	8	13	theme	novel	1610:1614	arg1	coatings					1632:1639	novel multifunctional coatings	1610:1639	novel multifunctional coatings	1610:1639	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	5	14	theme	preservation	911:922	arg1	performances					924:935	The preservation performances	907:935	The preservation performances of composite coatings	907:957	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	5	15	theme	samples	1065:1071	arg1	pH					1029:1030	albumen pH	1021:1030	albumen pH	1021:1030	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	5	15	theme	samples	1065:1071	arg1	morphologies					1045:1056	eggshell morphologies	1036:1056	eggshell morphologies of the samples	1036:1071	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	5	15	theme	samples	1065:1071	arg1	parameters					984:993	various parameters	976:993	various parameters	976:993	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	5	15	theme	samples	1065:1071	arg1	loss					1003:1006	weight loss	996:1006	weight loss	996:1006	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	5	15	theme	samples	1065:1071	arg1	unit					1015:1018	Haugh unit	1009:1018	Haugh unit	1009:1018	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	4	16	theme	composite	847:855	arg1	particles					857:865	composite particles	847:865	composite particles	847:865	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	3	17	theme	coating	517:523	arg1	materials					525:533	the existing chitosan-based coating materials	489:533	the existing chitosan-based coating materials	489:533	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	4	18	used	used	799:802	arg2	images					788:793	Electron microscope (SEM) images	762:793	Electron microscope (SEM) images	762:793	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	8	19	from	Methods	1375:1381	arg1	study					1391:1395	this study	1386:1395	this study	1386:1395	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	7	20	theme	existing	1283:1290	arg1	species					1301:1307	existing particle species	1283:1307	existing particle species	1283:1307	The performances of chitosan coating, however, are presently limited by the existing particle species and concentration, which requires optimization in future studies.					
34279501	4	21	theme	Electron	762:769	arg1	SEM					783:785	SEM	783:785	SEM	783:785	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	4	21	theme	Electron	762:769	arg1	microscope					771:780	Electron microscope	762:780	Electron microscope (SEM) images	762:793	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	6	22	theme	preservation	1178:1189	arg1	period					1191:1196	the preservation period	1174:1196	the preservation period	1174:1196	Adoption of Ag/TiO2 composites contributes to a synergistic effect to chitosan, which could prolong the preservation period further.					
34279501	0	23	theme	Chitosan-based	59:72	arg1	Coatings					74:81	Chitosan-based Coatings	59:81	Chitosan-based Coatings	59:81	Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances.					
34279501	3	24	theme	existing	493:500	arg1	materials					525:533	the existing chitosan-based coating materials	489:533	the existing chitosan-based coating materials	489:533	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	1	25	theme	vitamins	180:187	arg1	source					146:151	an excellent source	133:151	an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world	133:256	Eggs are an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world.					
34279501	1	25	theme	vitamins	180:187	arg1	Eggs					124:127	Eggs	124:127	Eggs	124:127	Eggs are an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world.					
34279501	2	26	theme	egg	404:406	arg1	deterioration					408:420	egg deterioration	404:420	egg deterioration	404:420	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	3	27	dep	stability	456:464	arg1	the					452:454	the	452:454	the	452:454	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	7	28	from	optimization	1343:1354	arg1	studies					1366:1372	future studies	1359:1372	future studies	1359:1372	The performances of chitosan coating, however, are presently limited by the existing particle species and concentration, which requires optimization in future studies.					
34279501	7	29	theme	particle	1292:1299	arg1	species					1301:1307	existing particle species	1283:1307	existing particle species	1283:1307	The performances of chitosan coating, however, are presently limited by the existing particle species and concentration, which requires optimization in future studies.					
34279501	4	30	dep	structure	819:827	arg1	the					815:817	the	815:817	the	815:817	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	8	31	theme	combinative	1529:1539	arg1	effects					1541:1547	the combinative effects	1525:1547	the combinative effects of the nanoparticle	1525:1567	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	0	32	theme	Dioxide	37:43	arg1	Composites					45:54	Silver/Titanium Dioxide Composites	21:54	Silver/Titanium Dioxide Composites	21:54	Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances.					
34279501	3	33	theme	egg	746:748	arg1	life					756:759	the egg shelf life	742:759	the egg shelf life	742:759	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	4	34	theme	eggshells	896:904	arg1	structure					819:827	structure	819:827	structure	819:827	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	4	34	theme	eggshells	896:904	arg1	morphology					875:884	the morphology	871:884	the morphology of coated eggshells	871:904	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	4	34	theme	eggshells	896:904	arg1	morphology					833:842	morphology	833:842	morphology	833:842	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	7	35	theme	future	1359:1364	arg1	studies					1366:1372	future studies	1359:1372	future studies	1359:1372	The performances of chitosan coating, however, are presently limited by the existing particle species and concentration, which requires optimization in future studies.					
34279501	3	36	theme	materials	525:533	arg1	sterilizability					470:484	sterilizability	470:484	sterilizability	470:484	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	3	36	theme	materials	525:533	arg1	stability					456:464	stability	456:464	stability	456:464	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	5	37	theme	coatings	950:957	arg1	performances					924:935	The preservation performances	907:935	The preservation performances of composite coatings	907:957	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	0	38	theme	Silver/Titanium	21:35	arg1	Composites					45:54	Silver/Titanium Dioxide Composites	21:54	Silver/Titanium Dioxide Composites	21:54	Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances.					
34279501	4	39	theme	coated	889:894	arg1	eggshells					896:904	coated eggshells	889:904	coated eggshells	889:904	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	3	40	theme	dioxide	645:651	arg1	composites					663:672	silver/titanium dioxide (Ag/TiO2) composites	629:672	silver/titanium dioxide (Ag/TiO2) composites	629:672	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	5	41	theme	composite	940:948	arg1	coatings					950:957	composite coatings	940:957	composite coatings	940:957	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	1	42	theme	proteins	156:163	arg1	source					146:151	an excellent source	133:151	an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world	133:256	Eggs are an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world.					
34279501	1	42	theme	proteins	156:163	arg1	Eggs					124:127	Eggs	124:127	Eggs	124:127	Eggs are an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world.					
34279501	2	43	theme	loss	435:438	arg1	acceleration					388:399	the acceleration	384:399	the acceleration of egg deterioration and economic loss	384:438	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	7	44	theme	coating	1236:1242	arg1	performances					1211:1222	The performances	1207:1222	The performances of chitosan coating	1207:1242	The performances of chitosan coating, however, are presently limited by the existing particle species and concentration, which requires optimization in future studies.					
34279501	0	45	theme	Composites	45:54	arg1	Functionalization					0:16	Functionalization	0:16	Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances.	0:122	Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances.					
34279501	8	46	theme	nanoparticle	1556:1567	arg1	effects					1541:1547	the combinative effects	1525:1547	the combinative effects of the nanoparticle	1525:1567	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	2	47	theme	economic	426:433	arg1	loss					435:438	economic loss	426:438	economic loss	426:438	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	7	48	dep	species	1301:1307	arg1	the					1279:1281	the	1279:1281	the	1279:1281	The performances of chitosan coating, however, are presently limited by the existing particle species and concentration, which requires optimization in future studies.					
34279501	2	49	theme	CO2	366:368	arg1	escape					356:361	the escape	352:361	the escape of CO2	352:368	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	2	49	theme	CO2	366:368	arg1	loss					331:334	the loss	327:334	the loss of moisture	327:346	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	3	50	theme	silver/titanium	629:643	arg1	dioxide					645:651	silver/titanium dioxide	629:651	silver/titanium dioxide (Ag/TiO2) composites	629:672	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	3	50	theme	silver/titanium	629:643	arg1	Ag/TiO2					654:660	Ag/TiO2	654:660	Ag/TiO2	654:660	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	3	51	theme	novel	550:554	arg1	nano-composites					572:586	novel multifunctional nano-composites	550:586	novel multifunctional nano-composites for anti-bacterial and egg preservation	550:626	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	2	52	theme	deterioration	408:420	arg1	acceleration					388:399	the acceleration	384:399	the acceleration of egg deterioration and economic loss	384:438	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	3	53	theme	shelf	750:754	arg1	life					756:759	the egg shelf life	742:759	the egg shelf life	742:759	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	2	54	dep	micro-pores	263:273	arg1	The					259:261	The	259:261	The	259:261	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	8	55	theme	novel	1405:1409	arg1	materials					1419:1427	novel coating materials	1405:1427	novel coating materials	1405:1427	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	3	56	theme	multifunctional	556:570	arg1	nano-composites					572:586	novel multifunctional nano-composites	550:586	novel multifunctional nano-composites for anti-bacterial and egg preservation	550:626	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	1	57	theme	excellent	136:144	arg1	source					146:151	an excellent source	133:151	an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world	133:256	Eggs are an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world.					
34279501	1	57	theme	excellent	136:144	arg1	Eggs					124:127	Eggs	124:127	Eggs	124:127	Eggs are an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world.					
34279501	2	58	from	micro-pores	263:273	arg1	eggshells					299:307	the eggshells	295:307	the eggshells	295:307	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	3	59	theme	life	756:759	arg1	extension					729:737	the extension	725:737	the extension of the egg shelf life	725:759	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	5	60	theme	albumen	1021:1027	arg1	pH					1029:1030	albumen pH	1021:1030	albumen pH	1021:1030	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	3	61	theme	egg	611:613	arg1	preservation					615:626	anti-bacterial and egg preservation	592:626	anti-bacterial and egg preservation	592:626	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	6	62	theme	composites	1094:1103	arg1	Adoption					1074:1081	Adoption	1074:1081	Adoption of Ag/TiO2 composites	1074:1103	Adoption of Ag/TiO2 composites contributes to a synergistic effect to chitosan, which could prolong the preservation period further.					
34279501	6	63	theme	synergistic	1122:1132	arg1	effect					1134:1139	a synergistic effect	1120:1139	a synergistic effect	1120:1139	Adoption of Ag/TiO2 composites contributes to a synergistic effect to chitosan, which could prolong the preservation period further.					
34279501	8	64	theme	specific	1463:1470	arg1	nanoparticles					1472:1484	specific nanoparticles	1463:1484	specific nanoparticles	1463:1484	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	4	65	theme	microscope	771:780	arg1	images					788:793	Electron microscope (SEM) images	762:793	Electron microscope (SEM) images	762:793	Electron microscope (SEM) images are used to analyze the structure and morphology of composite particles and the morphology of coated eggshells.					
34279501	6	66	theme	Ag/TiO2	1086:1092	arg1	composites					1094:1103	Ag/TiO2 composites	1086:1103	Ag/TiO2 composites	1086:1103	Adoption of Ag/TiO2 composites contributes to a synergistic effect to chitosan, which could prolong the preservation period further.					
34279501	2	67	from	micro-cracks	279:290	arg1	eggshells					299:307	the eggshells	295:307	the eggshells	295:307	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	1	68	theme	daily	228:232	arg1	all					239:241	daily diet all	228:241	daily diet all over the world	228:256	Eggs are an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world.					
34279501	3	69	theme	anti-bacterial	592:605	arg1	preservation					615:626	anti-bacterial and egg preservation	592:626	anti-bacterial and egg preservation	592:626	To enhance the stability and sterilizability of the existing chitosan-based coating materials and to develop novel multifunctional nano-composites for anti-bacterial and egg preservation, silver/titanium dioxide (Ag/TiO2) composites are synthesized and applied to modify chitosan for the extension of the egg shelf life.					
34279501	2	70	theme	moisture	339:346	arg1	escape					356:361	the escape	352:361	the escape of CO2	352:368	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	2	70	theme	moisture	339:346	arg1	loss					331:334	the loss	327:334	the loss of moisture	327:346	The micro-pores and micro-cracks on the eggshells, however, lead to the loss of moisture and the escape of CO2, resulting in the acceleration of egg deterioration and economic loss.					
34279501	8	71	theme	multifunctional	1616:1630	arg1	coatings					1632:1639	novel multifunctional coatings	1610:1639	novel multifunctional coatings	1610:1639	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
34279501	1	72	theme	diet	234:237	arg1	all					239:241	daily diet all	228:241	daily diet all over the world	228:256	Eggs are an excellent source of proteins, minerals, and vitamins, which have been popularly consumed in daily diet all over the world.					
34279501	5	73	theme	various	976:982	arg1	parameters					984:993	various parameters	976:993	various parameters	976:993	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	5	74	theme	weight	996:1001	arg1	loss					1003:1006	weight loss	996:1006	weight loss	996:1006	The preservation performances of composite coatings are evaluated by various parameters: weight loss, Haugh unit, albumen pH and eggshell morphologies of the samples.					
34279501	8	75	dep	precursor	1503:1511	arg1	achieve					1517:1523	achieve	1517:1523	to achieve the combinative effects of the nanoparticle	1514:1567	Methods in this study examine novel coating materials, which could be created by adding specific nanoparticles into the coating precursor, to achieve the combinative effects of the nanoparticle and the precursor, as well as to prepare novel multifunctional coatings in the field of food preservation.					
33711367	6	0	theme	microscopy	925:934	arg1	images					936:941	transmission electron microscopy images	903:941	transmission electron microscopy images	903:941	The CNFs produced had fibre diameters between 10 and 80 nm and transmission electron microscopy images showed that the CNFs were highly entangled hence forming a web like structure.					
33711367	5	1	theme	packaging	653:661	arg1	applications					663:674	food packaging applications	648:674	food packaging applications	648:674	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	8	2	theme	thermal	1265:1271	arg1	properties					1273:1282	the thermal properties	1261:1282	the thermal properties of the films	1261:1295	The incorporation of CNFs improved the WVP of the films but did not show any significant effect on the thermal properties of the films.					
33711367	0	3	theme	barrier	84:90	arg1	properties					92:101	barrier properties	84:101	barrier properties	84:101	Cellulose nanofibrils reinforced xylan-alginate composites: Mechanical, thermal and barrier properties.					
33711367	6	4	theme	electron	916:923	arg1	microscopy					925:934	transmission electron microscopy	903:934	transmission electron microscopy images	903:941	The CNFs produced had fibre diameters between 10 and 80 nm and transmission electron microscopy images showed that the CNFs were highly entangled hence forming a web like structure.					
33711367	7	5	theme	Young	1132:1136	arg1	modulus					1140:1146	Young's modulus	1132:1146	Young's modulus	1132:1146	It was found that the incorporation of CNFs into the xylan-alginate matrix increased the tensile strength and Young's modulus of the films.					
33711367	1	6	theme	effective	151:159	arg1	nanofibrils					114:124	Cellulose nanofibrils	104:124	Cellulose nanofibrils (CNFs)	104:131	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	1	6	theme	effective	151:159	arg1	material					175:182	an effective reinforcement material	148:182	an effective reinforcement material for biopolymer films intended for food packaging applications	148:244	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	6	7	theme	transmission	903:914	arg1	microscopy					925:934	transmission electron microscopy	903:934	transmission electron microscopy images	903:941	The CNFs produced had fibre diameters between 10 and 80 nm and transmission electron microscopy images showed that the CNFs were highly entangled hence forming a web like structure.					
33711367	7	8	theme	tensile	1111:1117	arg1	strength					1119:1126	tensile strength	1111:1126	tensile strength	1111:1126	It was found that the incorporation of CNFs into the xylan-alginate matrix increased the tensile strength and Young's modulus of the films.					
33711367	7	9	dep	strength	1119:1126	arg1	the					1107:1109	the	1107:1109	the	1107:1109	It was found that the incorporation of CNFs into the xylan-alginate matrix increased the tensile strength and Young's modulus of the films.					
33711367	5	10	theme	CNFs	612:615	arg1	films					638:642	the CNFs reinforced composite films	608:642	the CNFs reinforced composite films	608:642	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	6	11	contain	had	858:860	arg1	CNFs					844:847	The CNFs	840:847	The CNFs produced	840:856	The CNFs produced had fibre diameters between 10 and 80 nm and transmission electron microscopy images showed that the CNFs were highly entangled hence forming a web like structure.					
33711367	6	11	contain	had	858:860	arg2	diameters					868:876	fibre diameters	862:876	fibre diameters between 10 and 80 nm	862:897	The CNFs produced had fibre diameters between 10 and 80 nm and transmission electron microscopy images showed that the CNFs were highly entangled hence forming a web like structure.					
33711367	5	12	theme	composite	628:636	arg1	films					638:642	the CNFs reinforced composite films	608:642	the CNFs reinforced composite films	608:642	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	5	13	theme	reinforced	617:626	arg1	films					638:642	the CNFs reinforced composite films	608:642	the CNFs reinforced composite films	608:642	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	1	14	theme	reinforcement	161:173	arg1	nanofibrils					114:124	Cellulose nanofibrils	104:124	Cellulose nanofibrils (CNFs)	104:131	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	1	14	theme	reinforcement	161:173	arg1	material					175:182	an effective reinforcement material	148:182	an effective reinforcement material for biopolymer films intended for food packaging applications	148:244	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	5	15	theme	films	638:642	arg1	suitability					593:603	The suitability	589:603	The suitability of the CNFs reinforced composite films for food packaging applications	589:674	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	1	16	used	used	140:143	arg2	CNFs					127:130	CNFs	127:130	CNFs	127:130	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	1	16	used	used	140:143	arg2	nanofibrils					114:124	Cellulose nanofibrils	104:124	Cellulose nanofibrils (CNFs)	104:131	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	1	16	used	used	140:143	arg2	material					175:182	an effective reinforcement material	148:182	an effective reinforcement material for biopolymer films intended for food packaging applications	148:244	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	8	17	theme	CNFs	1183:1186	arg1	incorporation					1166:1178	The incorporation	1162:1178	The incorporation of CNFs	1162:1186	The incorporation of CNFs improved the WVP of the films but did not show any significant effect on the thermal properties of the films.					
33711367	7	18	theme	xylan-alginate	1075:1088	arg1	matrix					1090:1095	the xylan-alginate matrix	1071:1095	the xylan-alginate matrix	1071:1095	It was found that the incorporation of CNFs into the xylan-alginate matrix increased the tensile strength and Young's modulus of the films.					
33711367	3	19	theme	stalk	429:433	arg1	residues					441:448	maize stalk waste residues	423:448	maize stalk waste residues	423:448	CNFs was produced from maize stalk waste residues through a combination of chemical and mechanical treatment.					
33711367	0	20	theme	Cellulose	0:8	arg1	nanofibrils					10:20	Cellulose nanofibrils	0:20	Cellulose nanofibrils	0:20	Cellulose nanofibrils reinforced xylan-alginate composites: Mechanical, thermal and barrier properties.					
33711367	5	21	theme	food	648:651	arg1	applications					663:674	food packaging applications	648:674	food packaging applications	648:674	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	7	22	theme	CNFs	1061:1064	arg1	incorporation					1044:1056	the incorporation	1040:1056	the incorporation of CNFs into the xylan-alginate matrix	1040:1095	It was found that the incorporation of CNFs into the xylan-alginate matrix increased the tensile strength and Young's modulus of the films.					
33711367	2	23	theme	barrier	303:309	arg1	properties					311:320	the mechanical and barrier properties	284:320	properties	311:320	The aim of this study was to improve the mechanical and barrier properties of xylan-alginate films by incorporating CNFs into the xylan-alginate matrix.					
33711367	3	24	theme	waste	435:439	arg1	residues					441:448	maize stalk waste residues	423:448	maize stalk waste residues	423:448	CNFs was produced from maize stalk waste residues through a combination of chemical and mechanical treatment.					
33711367	0	25	theme	xylan-alginate	33:46	arg1	composites					48:57	xylan-alginate composites	33:57	xylan-alginate composites: Mechanical, thermal and barrier properties	33:101	Cellulose nanofibrils reinforced xylan-alginate composites: Mechanical, thermal and barrier properties.					
33711367	8	26	theme	significant	1239:1249	arg1	effect					1251:1256	any significant effect	1235:1256	any significant effect on the thermal properties of the films	1235:1295	The incorporation of CNFs improved the WVP of the films but did not show any significant effect on the thermal properties of the films.					
33711367	1	27	theme	Cellulose	104:112	arg1	CNFs					127:130	CNFs	127:130	CNFs	127:130	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	1	27	theme	Cellulose	104:112	arg1	nanofibrils					114:124	Cellulose nanofibrils	104:124	Cellulose nanofibrils (CNFs)	104:131	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	1	27	theme	Cellulose	104:112	arg1	material					175:182	an effective reinforcement material	148:182	an effective reinforcement material for biopolymer films intended for food packaging applications	148:244	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	5	28	theme	thermal	720:726	arg1	properties					740:749	the mechanical, thermal and optical properties	704:749	the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films	704:837	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	2	29	theme	mechanical	288:297	arg1	properties					311:320	the mechanical and barrier properties	284:320	properties	311:320	The aim of this study was to improve the mechanical and barrier properties of xylan-alginate films by incorporating CNFs into the xylan-alginate matrix.					
33711367	3	30	theme	chemical	475:482	arg1	treatment					499:507	chemical and mechanical treatment	475:507	chemical and mechanical treatment	475:507	CNFs was produced from maize stalk waste residues through a combination of chemical and mechanical treatment.					
33711367	2	31	theme	xylan-alginate	377:390	arg1	matrix					392:397	the xylan-alginate matrix	373:397	the xylan-alginate matrix	373:397	The aim of this study was to improve the mechanical and barrier properties of xylan-alginate films by incorporating CNFs into the xylan-alginate matrix.					
33711367	3	32	theme	maize	423:427	arg1	residues					441:448	maize stalk waste residues	423:448	maize stalk waste residues	423:448	CNFs was produced from maize stalk waste residues through a combination of chemical and mechanical treatment.					
33711367	5	33	theme	optical	732:738	arg1	properties					740:749	the mechanical, thermal and optical properties	704:749	the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films	704:837	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	6	34	theme	fibre	862:866	arg1	diameters					868:876	fibre diameters	862:876	fibre diameters between 10 and 80 nm	862:897	The CNFs produced had fibre diameters between 10 and 80 nm and transmission electron microscopy images showed that the CNFs were highly entangled hence forming a web like structure.					
33711367	7	35	theme	films	1155:1159	arg1	strength					1119:1126	tensile strength	1111:1126	tensile strength	1111:1126	It was found that the incorporation of CNFs into the xylan-alginate matrix increased the tensile strength and Young's modulus of the films.					
33711367	7	35	theme	films	1155:1159	arg1	modulus					1140:1146	Young's modulus	1132:1146	Young's modulus	1132:1146	It was found that the incorporation of CNFs into the xylan-alginate matrix increased the tensile strength and Young's modulus of the films.					
33711367	1	36	theme	food	218:221	arg1	applications					233:244	food packaging applications	218:244	food packaging applications	218:244	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	5	37	theme	water	800:804	arg1	permeability					813:824	water vapour permeability	800:824	water vapour permeability	800:824	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	1	38	theme	biopolymer	188:197	arg1	films					199:203	biopolymer films	188:203	biopolymer films intended for food packaging applications	188:244	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	3	39	theme	treatment	499:507	arg1	combination					460:470	a combination	458:470	a combination of chemical and mechanical treatment	458:507	CNFs was produced from maize stalk waste residues through a combination of chemical and mechanical treatment.					
33711367	5	40	theme	vapour	806:811	arg1	permeability					813:824	water vapour permeability	800:824	water vapour permeability	800:824	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	0	41	dep	composites	48:57	arg1	thermal					72:78	thermal	72:78	thermal	72:78	Cellulose nanofibrils reinforced xylan-alginate composites: Mechanical, thermal and barrier properties.					
33711367	0	41	dep	composites	48:57	arg1	properties					92:101	barrier properties	84:101	barrier properties	84:101	Cellulose nanofibrils reinforced xylan-alginate composites: Mechanical, thermal and barrier properties.					
33711367	0	41	dep	composites	48:57	arg1	Mechanical					60:69	Mechanical	60:69	Mechanical	60:69	Cellulose nanofibrils reinforced xylan-alginate composites: Mechanical, thermal and barrier properties.					
33711367	2	42	theme	films	340:344	arg1	properties					311:320	the mechanical and barrier properties	284:320	properties	311:320	The aim of this study was to improve the mechanical and barrier properties of xylan-alginate films by incorporating CNFs into the xylan-alginate matrix.					
33711367	4	43	theme	xylan-alginate	545:558	arg1	matrix					560:565	the xylan-alginate matrix	541:565	the xylan-alginate matrix between 1 and 10 wt%	541:586	The CNFs was incorporated into the xylan-alginate matrix between 1 and 10 wt%.					
33711367	2	44	theme	study	263:267	arg1	aim					251:253	The aim	247:253	The aim of this study	247:267	The aim of this study was to improve the mechanical and barrier properties of xylan-alginate films by incorporating CNFs into the xylan-alginate matrix.					
33711367	2	45	theme	xylan-alginate	325:338	arg1	films					340:344	xylan-alginate films	325:344	xylan-alginate films	325:344	The aim of this study was to improve the mechanical and barrier properties of xylan-alginate films by incorporating CNFs into the xylan-alginate matrix.					
33711367	1	46	theme	packaging	223:231	arg1	applications					233:244	food packaging applications	218:244	food packaging applications	218:244	Cellulose nanofibrils (CNFs) can be used as an effective reinforcement material for biopolymer films intended for food packaging applications.					
33711367	5	47	theme	mechanical	708:717	arg1	properties					740:749	the mechanical, thermal and optical properties	704:749	the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films	704:837	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	3	48	theme	mechanical	488:497	arg1	treatment					499:507	chemical and mechanical treatment	475:507	chemical and mechanical treatment	475:507	CNFs was produced from maize stalk waste residues through a combination of chemical and mechanical treatment.					
33711367	8	49	theme	films	1212:1216	arg1	WVP					1201:1203	the WVP	1197:1203	the WVP of the films	1197:1216	The incorporation of CNFs improved the WVP of the films but did not show any significant effect on the thermal properties of the films.					
33711367	8	50	from	effect	1251:1256	arg1	properties					1273:1282	the thermal properties	1261:1282	the thermal properties of the films	1261:1295	The incorporation of CNFs improved the WVP of the films but did not show any significant effect on the thermal properties of the films.					
33711367	8	51	theme	films	1291:1295	arg1	properties					1273:1282	the thermal properties	1261:1282	the thermal properties of the films	1261:1295	The incorporation of CNFs improved the WVP of the films but did not show any significant effect on the thermal properties of the films.					
33711367	5	52	theme	moisture	766:773	arg1	sorption					775:782	the moisture sorption	762:782	the moisture sorption	762:782	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	5	53	theme	films	833:837	arg1	properties					740:749	the mechanical, thermal and optical properties	704:749	the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films	704:837	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	5	53	theme	films	833:837	arg1	sorption					775:782	the moisture sorption	762:782	the moisture sorption	762:782	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	5	53	theme	films	833:837	arg1	solubility					785:794	solubility	785:794	solubility	785:794	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
33711367	5	53	theme	films	833:837	arg1	permeability					813:824	water vapour permeability	800:824	water vapour permeability	800:824	The suitability of the CNFs reinforced composite films for food packaging applications was investigated by testing the mechanical, thermal and optical properties as well as the moisture sorption, solubility and water vapour permeability of the films.					
32635318	6	0	theme	triple	1007:1012	arg1	bonds					1022:1026	triple dynamic bonds	1007:1026	triple dynamic bonds	1007:1026	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	7	1	theme	satisfying	1224:1233	arg1	cytocompatibility					1235:1251	satisfying cytocompatibility	1224:1251	satisfying cytocompatibility of the CMC-OSA-DTP hydrogel	1224:1279	In addition, the CCK-8 and live/dead assays demonstrated satisfying cytocompatibility of the CMC-OSA-DTP hydrogel in vitro.					
32635318	5	2	theme	polymers	802:809	arg1	OSA					864:866	OSA	864:866	OSA	864:866	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	2	theme	polymers	802:809	arg1	DTP					919:921	DTP	919:921	DTP	919:921	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	2	theme	polymers	802:809	arg1	kinds					762:766	Two kinds	758:766	Two kinds of natural polysaccharide derived polymers	758:809	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	2	theme	polymers	802:809	arg1	alginate					854:861	oxidized alginate	845:861	oxidized alginate (OSA)	845:867	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	2	theme	polymers	802:809	arg1	dihydrazide					906:916	3,3'-dithiopropionic acid dihydrazide	880:916	3,3'-dithiopropionic acid dihydrazide (DTP)	880:922	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	8	3	theme	applications	1467:1478	arg1	variety					1445:1451	a variety	1443:1451	a variety of biomedical applications	1443:1478	Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.					
32635318	8	3	theme	applications	1467:1478	arg1	applications					1467:1478	biomedical applications	1456:1478	biomedical applications	1456:1478	Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.					
32635318	5	4	theme	oxidized	845:852	arg1	OSA					864:866	OSA	864:866	OSA	864:866	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	4	theme	oxidized	845:852	arg1	alginate					854:861	oxidized alginate	845:861	oxidized alginate (OSA)	845:867	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	6	5	theme	stimuli-responsive	1091:1108	arg1	property					1110:1117	smart redox and pH stimuli-responsive property	1072:1117	property	1110:1117	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	0	6	theme	Dynamic	73:79	arg1	Bonds					81:85	Triple Dynamic Bonds	66:85	Triple Dynamic Bonds	66:85	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds.					
32635318	8	7	theme	easy-fabricated	1332:1346	arg1	hydrogel					1369:1376	this easy-fabricated and multi-functional hydrogel	1327:1376	this easy-fabricated and multi-functional hydrogel	1327:1376	Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.					
32635318	1	8	theme	clinical	172:179	arg1	applications					181:192	clinical applications	172:192	clinical applications	172:192	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	6	9	theme	pH	1088:1089	arg1	property					1110:1117	smart redox and pH stimuli-responsive property	1072:1117	property	1110:1117	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	5	10	dep	derived	794:800	arg1	polysaccharide					779:792	natural polysaccharide	771:792	natural polysaccharide	771:792	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	3	11	theme	growing	593:599	arg1	development					469:479	The development	465:479	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties	465:586	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	3	11	theme	growing	593:599	arg1	urgency					601:607	a growing urgency	591:607	a growing urgency toward biomedical applications	591:638	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	4	12	theme	simple	675:680	arg1	approach					696:703	a simple and effective approach	673:703	a simple and effective approach to fabricate multi-functional CMC-OSA-DTP hydrogels	673:755	In this contribution, we report a simple and effective approach to fabricate multi-functional CMC-OSA-DTP hydrogels.					
32635318	5	13	theme	carboxymethyl	812:824	arg1	CMC					836:838	CMC	836:838	CMC	836:838	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	13	theme	carboxymethyl	812:824	arg1	chitosan					826:833	carboxymethyl chitosan	812:833	carboxymethyl chitosan (CMC)	812:839	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	8	14	from	biomaterial	1428:1438	arg1	variety					1445:1451	a variety	1443:1451	a variety of biomedical applications	1443:1478	Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.					
32635318	8	14	from	biomaterial	1428:1438	arg1	applications					1467:1478	biomedical applications	1456:1478	biomedical applications	1456:1478	Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.					
32635318	8	15	theme	injectable	1417:1426	arg1	biomaterial					1428:1438	an injectable biomaterial	1414:1438	an injectable biomaterial in a variety of biomedical applications	1414:1478	Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.					
32635318	5	16	link	derived	794:800	arg1	polymers					802:809	natural polysaccharide derived polymers	771:809	natural polysaccharide derived polymers	771:809	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	3	17	theme	biocompatibility	536:551	arg1	combination					521:531	a combination	519:531	a combination of biocompatibility and adequate functional properties	519:586	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	2	18	theme	injectable	317:326	arg1	hydrogels					328:336	natural biomaterials-based injectable hydrogels	290:336	natural biomaterials-based injectable hydrogels with desirable nontoxicity	290:363	Nevertheless, natural biomaterials-based injectable hydrogels with desirable nontoxicity are suffering from limited functions, failing to fulfill the requirements of clinical biomaterials.					
32635318	3	19	theme	novel	484:488	arg1	biomaterials					501:512	novel injectable biomaterials	484:512	novel injectable biomaterials with a combination of biocompatibility and adequate functional properties	484:586	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	2	20	theme	biomaterials	451:462	arg1	requirements					426:437	the requirements	422:437	the requirements of clinical biomaterials	422:462	Nevertheless, natural biomaterials-based injectable hydrogels with desirable nontoxicity are suffering from limited functions, failing to fulfill the requirements of clinical biomaterials.					
32635318	5	21	theme	acid	901:904	arg1	DTP					919:921	DTP	919:921	DTP	919:921	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	21	theme	acid	901:904	arg1	dihydrazide					906:916	3,3'-dithiopropionic acid dihydrazide	880:916	3,3'-dithiopropionic acid dihydrazide (DTP)	880:922	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	7	22	theme	live/dead	1194:1202	arg1	assays					1204:1209	the CCK-8 and live/dead assays	1180:1209	the CCK-8 and live/dead assays	1180:1209	In addition, the CCK-8 and live/dead assays demonstrated satisfying cytocompatibility of the CMC-OSA-DTP hydrogel in vitro.					
32635318	2	23	theme	biomaterials-based	298:315	arg1	hydrogels					328:336	natural biomaterials-based injectable hydrogels	290:336	natural biomaterials-based injectable hydrogels with desirable nontoxicity	290:363	Nevertheless, natural biomaterials-based injectable hydrogels with desirable nontoxicity are suffering from limited functions, failing to fulfill the requirements of clinical biomaterials.					
32635318	2	24	theme	clinical	442:449	arg1	biomaterials					451:462	clinical biomaterials	442:462	clinical biomaterials	442:462	Nevertheless, natural biomaterials-based injectable hydrogels with desirable nontoxicity are suffering from limited functions, failing to fulfill the requirements of clinical biomaterials.					
32635318	0	25	theme	Biocompatible	2:14	arg1	Hydrogel					52:59	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel	0:59	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds	0:85	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds.					
32635318	6	26	theme	CMC-OSA-DTP	1041:1051	arg1	hydrogel					1053:1060	this unique CMC-OSA-DTP hydrogel	1029:1060	this unique CMC-OSA-DTP hydrogel	1029:1060	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	5	27	dep	along	869:873	arg1	with					875:878	with	875:878	with	875:878	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	1	28	theme	due	195:197	arg1	applications					181:192	clinical applications	172:192	clinical applications	172:192	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	6	29	theme	smart	1072:1076	arg1	redox					1078:1082	smart redox and pH stimuli-responsive property	1072:1117	redox	1078:1082	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	6	29	theme	smart	1072:1076	arg1	injectability					1120:1132	injectability	1120:1132	injectability	1120:1132	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	7	30	theme	CCK-8	1184:1188	arg1	assays					1204:1209	the CCK-8 and live/dead assays	1180:1209	the CCK-8 and live/dead assays	1180:1209	In addition, the CCK-8 and live/dead assays demonstrated satisfying cytocompatibility of the CMC-OSA-DTP hydrogel in vitro.					
32635318	1	31	theme	Injectable	88:97	arg1	hydrogels					99:107	Injectable hydrogels	88:107	Injectable hydrogels	88:107	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	8	32	theme	great	1395:1399	arg1	potential					1401:1409	the great potential	1391:1409	the great potential as an injectable biomaterial in a variety of biomedical applications	1391:1478	Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.					
32635318	3	33	theme	injectable	490:499	arg1	biomaterials					501:512	novel injectable biomaterials	484:512	novel injectable biomaterials with a combination of biocompatibility and adequate functional properties	484:586	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	0	34	theme	Stimuli-Responsive	17:34	arg1	Hydrogel					52:59	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel	0:59	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds	0:85	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds.					
32635318	8	35	theme	biomedical	1456:1465	arg1	applications					1467:1478	biomedical applications	1456:1478	biomedical applications	1456:1478	Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.					
32635318	4	36	theme	multi-functional	718:733	arg1	hydrogels					747:755	multi-functional CMC-OSA-DTP hydrogels	718:755	multi-functional CMC-OSA-DTP hydrogels	718:755	In this contribution, we report a simple and effective approach to fabricate multi-functional CMC-OSA-DTP hydrogels.					
32635318	8	37	theme	attractive	1304:1313	arg1	properties					1315:1324	its attractive properties	1300:1324	its attractive properties	1300:1324	Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.					
32635318	5	38	used	utilized	929:936	arg2	DTP					919:921	DTP	919:921	DTP	919:921	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	38	used	utilized	929:936	arg2	CMC					836:838	CMC	836:838	CMC	836:838	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	38	used	utilized	929:936	arg2	kinds					762:766	Two kinds	758:766	Two kinds of natural polysaccharide derived polymers	758:809	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	38	used	utilized	929:936	arg2	chitosan					826:833	carboxymethyl chitosan	812:833	carboxymethyl chitosan (CMC)	812:839	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	38	used	utilized	929:936	arg2	alginate					854:861	oxidized alginate	845:861	oxidized alginate (OSA)	845:867	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	38	used	utilized	929:936	arg2	OSA					864:866	OSA	864:866	OSA	864:866	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	38	used	utilized	929:936	arg2	dihydrazide					906:916	3,3'-dithiopropionic acid dihydrazide	880:916	3,3'-dithiopropionic acid dihydrazide (DTP)	880:922	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	2	39	theme	desirable	343:351	arg1	nontoxicity					353:363	desirable nontoxicity	343:363	desirable nontoxicity	343:363	Nevertheless, natural biomaterials-based injectable hydrogels with desirable nontoxicity are suffering from limited functions, failing to fulfill the requirements of clinical biomaterials.					
32635318	7	40	theme	hydrogel	1272:1279	arg1	cytocompatibility					1235:1251	satisfying cytocompatibility	1224:1251	satisfying cytocompatibility of the CMC-OSA-DTP hydrogel	1224:1279	In addition, the CCK-8 and live/dead assays demonstrated satisfying cytocompatibility of the CMC-OSA-DTP hydrogel in vitro.					
32635318	1	41	theme	minimum	208:214	arg1	approach					236:243	their minimum invasive implanting approach	202:243	their minimum invasive implanting approach	202:243	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	7	42	theme	CMC-OSA-DTP	1260:1270	arg1	hydrogel					1272:1279	the CMC-OSA-DTP hydrogel	1256:1279	the CMC-OSA-DTP hydrogel	1256:1279	In addition, the CCK-8 and live/dead assays demonstrated satisfying cytocompatibility of the CMC-OSA-DTP hydrogel in vitro.					
32635318	3	43	theme	properties	577:586	arg1	combination					521:531	a combination	519:531	a combination of biocompatibility and adequate functional properties	519:586	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	1	44	theme	invasive	216:223	arg1	approach					236:243	their minimum invasive implanting approach	202:243	their minimum invasive implanting approach	202:243	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	3	45	theme	biomaterials	501:512	arg1	development					469:479	The development	465:479	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties	465:586	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	3	45	theme	biomaterials	501:512	arg1	urgency					601:607	a growing urgency	591:607	a growing urgency toward biomedical applications	591:638	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	6	46	theme	unique	1034:1039	arg1	hydrogel					1053:1060	this unique CMC-OSA-DTP hydrogel	1029:1060	this unique CMC-OSA-DTP hydrogel	1029:1060	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	5	47	theme	covalent	965:972	arg1	bonds					974:978	three dynamic covalent bonds	951:978	three dynamic covalent bonds	951:978	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	6	48	theme	self-healing	1145:1156	arg1	ability					1158:1164	self-healing ability	1145:1164	smart redox and pH stimuli-responsive property, injectability as well as self-healing ability	1072:1164	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	1	49	theme	implanting	225:234	arg1	approach					236:243	their minimum invasive implanting approach	202:243	their minimum invasive implanting approach	202:243	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	5	50	theme	3,3'-dithiopropionic	880:899	arg1	DTP					919:921	DTP	919:921	DTP	919:921	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	50	theme	3,3'-dithiopropionic	880:899	arg1	dihydrazide					906:916	3,3'-dithiopropionic acid dihydrazide	880:916	3,3'-dithiopropionic acid dihydrazide (DTP)	880:922	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	5	51	theme	natural	771:777	arg1	polysaccharide					779:792	natural polysaccharide	771:792	natural polysaccharide	771:792	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	6	52	contain	possessed	1062:1070	arg1	hydrogel					1053:1060	this unique CMC-OSA-DTP hydrogel	1029:1060	this unique CMC-OSA-DTP hydrogel	1029:1060	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	6	52	contain	possessed	1062:1070	arg2	injectability					1120:1132	injectability	1120:1132	injectability	1120:1132	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	6	52	contain	possessed	1062:1070	arg2	ability					1158:1164	self-healing ability	1145:1164	smart redox and pH stimuli-responsive property, injectability as well as self-healing ability	1072:1164	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	6	52	contain	possessed	1062:1070	arg2	property					1110:1117	smart redox and pH stimuli-responsive property	1072:1117	property	1110:1117	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	6	52	contain	possessed	1062:1070	arg2	redox					1078:1082	smart redox and pH stimuli-responsive property	1072:1117	redox	1078:1082	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	5	53	theme	derived	794:800	arg1	polymers					802:809	natural polysaccharide derived polymers	771:809	natural polysaccharide derived polymers	771:809	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	3	54	theme	functional	566:575	arg1	properties					577:586	adequate functional properties	557:586	adequate functional properties	557:586	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	0	55	theme	Injectable	41:50	arg1	Hydrogel					52:59	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel	0:59	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds	0:85	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds.					
32635318	1	56	theme	growing	124:130	arg1	interests					132:140	growing interests	124:140	growing interests	124:140	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	1	56	theme	growing	124:130	arg1	biomaterials					155:166	promising biomaterials	145:166	promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance	145:273	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	6	57	theme	bonds	1022:1026	arg1	existence					994:1002	the existence	990:1002	the existence of triple dynamic bonds	990:1026	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	3	58	with	biomaterials	501:512	arg1	combination					521:531	a combination	519:531	a combination of biocompatibility and adequate functional properties	519:586	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	2	59	theme	natural	290:296	arg1	hydrogels					328:336	natural biomaterials-based injectable hydrogels	290:336	natural biomaterials-based injectable hydrogels with desirable nontoxicity	290:363	Nevertheless, natural biomaterials-based injectable hydrogels with desirable nontoxicity are suffering from limited functions, failing to fulfill the requirements of clinical biomaterials.					
32635318	2	60	theme	limited	384:390	arg1	functions					392:400	limited functions	384:400	limited functions	384:400	Nevertheless, natural biomaterials-based injectable hydrogels with desirable nontoxicity are suffering from limited functions, failing to fulfill the requirements of clinical biomaterials.					
32635318	4	61	theme	effective	686:694	arg1	approach					696:703	a simple and effective approach	673:703	a simple and effective approach to fabricate multi-functional CMC-OSA-DTP hydrogels	673:755	In this contribution, we report a simple and effective approach to fabricate multi-functional CMC-OSA-DTP hydrogels.					
32635318	3	62	theme	biomedical	616:625	arg1	applications					627:638	biomedical applications	616:638	biomedical applications	616:638	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	8	63	theme	multi-functional	1352:1367	arg1	hydrogel					1369:1376	this easy-fabricated and multi-functional hydrogel	1327:1376	this easy-fabricated and multi-functional hydrogel	1327:1376	Based on its attractive properties, this easy-fabricated and multi-functional hydrogel demonstrated the great potential as an injectable biomaterial in a variety of biomedical applications.					
32635318	0	64	with	Hydrogel	52:59	arg1	Bonds					81:85	Triple Dynamic Bonds	66:85	Triple Dynamic Bonds	66:85	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds.					
32635318	3	65	theme	adequate	557:564	arg1	properties					577:586	adequate functional properties	557:586	adequate functional properties	557:586	The development of novel injectable biomaterials with a combination of biocompatibility and adequate functional properties is a growing urgency toward biomedical applications.					
32635318	0	66	theme	Triple	66:71	arg1	Bonds					81:85	Triple Dynamic Bonds	66:85	Triple Dynamic Bonds	66:85	A Biocompatible, Stimuli-Responsive, and Injectable Hydrogel with Triple Dynamic Bonds.					
32635318	4	67	theme	CMC-OSA-DTP	735:745	arg1	hydrogels					747:755	multi-functional CMC-OSA-DTP hydrogels	718:755	multi-functional CMC-OSA-DTP hydrogels	718:755	In this contribution, we report a simple and effective approach to fabricate multi-functional CMC-OSA-DTP hydrogels.					
32635318	1	68	theme	promising	145:153	arg1	interests					132:140	growing interests	124:140	growing interests	124:140	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	1	68	theme	promising	145:153	arg1	biomaterials					155:166	promising biomaterials	145:166	promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance	145:273	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	5	69	theme	dynamic	957:963	arg1	bonds					974:978	three dynamic covalent bonds	951:978	three dynamic covalent bonds	951:978	Two kinds of natural polysaccharide derived polymers, carboxymethyl chitosan (CMC) and oxidized alginate (OSA) along with 3,3'-dithiopropionic acid dihydrazide (DTP) were utilized to introduce three dynamic covalent bonds.					
32635318	1	70	theme	easy-handling	249:261	arg1	performance					263:273	easy-handling performance	249:273	easy-handling performance	249:273	Injectable hydrogels have attracted growing interests as promising biomaterials for clinical applications, due to their minimum invasive implanting approach and easy-handling performance.					
32635318	6	71	theme	dynamic	1014:1020	arg1	bonds					1022:1026	triple dynamic bonds	1007:1026	triple dynamic bonds	1007:1026	Owing to the existence of triple dynamic bonds, this unique CMC-OSA-DTP hydrogel possessed smart redox and pH stimuli-responsive property, injectability as well as self-healing ability.					
32635318	2	72	with	hydrogels	328:336	arg1	nontoxicity					353:363	desirable nontoxicity	343:363	desirable nontoxicity	343:363	Nevertheless, natural biomaterials-based injectable hydrogels with desirable nontoxicity are suffering from limited functions, failing to fulfill the requirements of clinical biomaterials.					
34923399	5	0	theme	steam	780:784	arg1	SEP					810:812	SEP	810:812	SEP	810:812	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP) resulted in the significant increases in their antioxidant activities in vitro and in vivo.					
34923399	5	0	theme	steam	780:784	arg1	pretreatment					796:807	the steam explosion pretreatment	776:807	the steam explosion pretreatment (SEP)	776:813	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP) resulted in the significant increases in their antioxidant activities in vitro and in vivo.					
34923399	6	1	dep	Achyranthis	1057:1067	arg1	bidentatae					1069:1078	bidentatae	1069:1078	bidentatae	1069:1078	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	3	2	theme	compositional	503:515	arg1	modification					517:528	the main compositional modification	494:528	the main compositional modification of polysaccharide	494:546	The monosaccharide analysis showed the main compositional modification of polysaccharide is the increase in the proportion of galacturonic acid, galactose, and arabinose.					
34923399	6	3	theme	antioxidant	1031:1041	arg1	activities					1043:1052	antioxidant activities	1031:1052	antioxidant activities	1031:1052	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	5	4	theme	explosion	786:794	arg1	SEP					810:812	SEP	810:812	SEP	810:812	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP) resulted in the significant increases in their antioxidant activities in vitro and in vivo.					
34923399	5	4	theme	explosion	786:794	arg1	pretreatment					796:807	the steam explosion pretreatment	776:807	the steam explosion pretreatment (SEP)	776:813	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP) resulted in the significant increases in their antioxidant activities in vitro and in vivo.					
34923399	2	5	theme	polysaccharide	352:365	arg1	yield					313:317	extraction yield	302:317	extraction yield	302:317	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	2	5	theme	polysaccharide	352:365	arg1	content					335:341	uronic acid content	323:341	uronic acid content	323:341	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	3	6	from	increase	555:562	arg1	proportion					571:580	the proportion	567:580	the proportion of galacturonic acid, galactose, and arabinose	567:627	The monosaccharide analysis showed the main compositional modification of polysaccharide is the increase in the proportion of galacturonic acid, galactose, and arabinose.					
34923399	1	7	theme	polysaccharide	256:269	arg1	extraction					271:280	the polysaccharide extraction	252:280	the polysaccharide extraction	252:280	Steam explosion technology was employed for the pretreatment of Achyranthis bidentatae radix (ABR) under mild conditions, followed by the polysaccharide extraction.					
34923399	1	8	theme	Steam	118:122	arg1	technology					134:143	Steam explosion technology	118:143	Steam explosion technology	118:143	Steam explosion technology was employed for the pretreatment of Achyranthis bidentatae radix (ABR) under mild conditions, followed by the polysaccharide extraction.					
34923399	0	9	theme	antioxidant	94:104	arg1	activities					106:115	its antioxidant activities	90:115	its antioxidant activities	90:115	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.					
34923399	6	10	theme	Strong	907:912	arg1	correlations					914:925	Strong correlations	907:925	Strong correlations	907:925	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	4	11	theme	spectra	702:708	arg1	analysis					669:676	the discriminant analysis	652:676	the discriminant analysis of the FT-IR and UV-vis spectra	652:708	It is consistent with the discriminant analysis of the FT-IR and UV-vis spectra.					
34923399	6	12	theme	structural	1000:1009	arg1	characteristics					1011:1025	structural characteristics	1000:1025	structural characteristics	1000:1025	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	4	13	theme	UV-vis	695:700	arg1	spectra					702:708	the FT-IR and UV-vis spectra	681:708	spectra	702:708	It is consistent with the discriminant analysis of the FT-IR and UV-vis spectra.					
34923399	1	14	theme	explosion	124:132	arg1	technology					134:143	Steam explosion technology	118:143	Steam explosion technology	118:143	Steam explosion technology was employed for the pretreatment of Achyranthis bidentatae radix (ABR) under mild conditions, followed by the polysaccharide extraction.					
34923399	3	15	theme	monosaccharide	463:476	arg1	analysis					478:485	The monosaccharide analysis	459:485	The monosaccharide analysis	459:485	The monosaccharide analysis showed the main compositional modification of polysaccharide is the increase in the proportion of galacturonic acid, galactose, and arabinose.					
34923399	4	16	theme	discriminant	656:667	arg1	analysis					669:676	the discriminant analysis	652:676	the discriminant analysis of the FT-IR and UV-vis spectra	652:708	It is consistent with the discriminant analysis of the FT-IR and UV-vis spectra.					
34923399	0	17	theme	explosion	6:14	arg1	pretreatment					16:27	Steam explosion pretreatment	0:27	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.	0:116	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.					
34923399	3	18	theme	polysaccharide	533:546	arg1	modification					517:528	the main compositional modification	494:528	the main compositional modification of polysaccharide	494:546	The monosaccharide analysis showed the main compositional modification of polysaccharide is the increase in the proportion of galacturonic acid, galactose, and arabinose.					
34923399	2	19	theme	uronic	323:328	arg1	content					335:341	uronic acid content	323:341	uronic acid content	323:341	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	1	20	theme	radix	205:209	arg1	pretreatment					166:177	the pretreatment	162:177	the pretreatment of Achyranthis bidentatae radix (ABR) under mild conditions	162:237	Steam explosion technology was employed for the pretreatment of Achyranthis bidentatae radix (ABR) under mild conditions, followed by the polysaccharide extraction.					
34923399	0	21	theme	Steam	0:4	arg1	pretreatment					16:27	Steam explosion pretreatment	0:27	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.	0:116	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.					
34923399	3	22	theme	galacturonic	585:596	arg1	acid					598:601	galacturonic acid	585:601	galacturonic acid	585:601	The monosaccharide analysis showed the main compositional modification of polysaccharide is the increase in the proportion of galacturonic acid, galactose, and arabinose.					
34923399	6	23	from	changes	985:991	arg1	characteristics					1011:1025	structural characteristics	1000:1025	structural characteristics	1000:1025	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	6	23	from	changes	985:991	arg1	activities					1043:1052	antioxidant activities	1031:1052	antioxidant activities	1031:1052	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	5	24	theme	significant	831:841	arg1	increases					843:851	the significant increases	827:851	the significant increases in their antioxidant activities in vitro and in vivo	827:904	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP) resulted in the significant increases in their antioxidant activities in vitro and in vivo.					
34923399	5	25	from	increases	843:851	arg1	activities					874:883	their antioxidant activities	856:883	their antioxidant activities	856:883	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP) resulted in the significant increases in their antioxidant activities in vitro and in vivo.					
34923399	2	26	theme	extraction	302:311	arg1	yield					313:317	extraction yield	302:317	extraction yield	302:317	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	2	27	theme	protein	411:417	arg1	content					419:425	the protein content	407:425	the protein content induced by the steam explosion	407:456	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	0	28	theme	bidentatae	44:53	arg1	radix					55:59	Achyranthis bidentatae radix	32:59	Achyranthis bidentatae radix	32:59	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.					
34923399	5	29	theme	structural	717:726	arg1	modifications					728:740	These structural modifications	711:740	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP)	711:813	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP) resulted in the significant increases in their antioxidant activities in vitro and in vivo.					
34923399	3	30	theme	galactose	604:612	arg1	proportion					571:580	the proportion	567:580	the proportion of galacturonic acid, galactose, and arabinose	567:627	The monosaccharide analysis showed the main compositional modification of polysaccharide is the increase in the proportion of galacturonic acid, galactose, and arabinose.					
34923399	6	31	theme	polysaccharides	1086:1100	arg1	characteristics					1011:1025	structural characteristics	1000:1025	structural characteristics	1000:1025	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	6	31	theme	polysaccharides	1086:1100	arg1	activities					1043:1052	antioxidant activities	1031:1052	antioxidant activities	1031:1052	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	0	32	theme	Achyranthis	32:42	arg1	radix					55:59	Achyranthis bidentatae radix	32:59	Achyranthis bidentatae radix	32:59	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.					
34923399	1	33	theme	mild	223:226	arg1	conditions					228:237	mild conditions	223:237	mild conditions	223:237	Steam explosion technology was employed for the pretreatment of Achyranthis bidentatae radix (ABR) under mild conditions, followed by the polysaccharide extraction.					
34923399	6	34	theme	radix	1080:1084	arg1	ABPS					1103:1106	ABPS	1103:1106	ABPS	1103:1106	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	6	34	theme	radix	1080:1084	arg1	polysaccharides					1086:1100	Achyranthis bidentatae radix polysaccharides	1057:1100	Achyranthis bidentatae radix polysaccharides (ABPS)	1057:1107	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	3	35	theme	acid	598:601	arg1	proportion					571:580	the proportion	567:580	the proportion of galacturonic acid, galactose, and arabinose	567:627	The monosaccharide analysis showed the main compositional modification of polysaccharide is the increase in the proportion of galacturonic acid, galactose, and arabinose.					
34923399	2	36	theme	crude	346:350	arg1	polysaccharide					352:365	crude polysaccharide	346:365	crude polysaccharide	346:365	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	0	37	theme	radix	55:59	arg1	pretreatment					16:27	Steam explosion pretreatment	0:27	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.	0:116	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.					
34923399	6	38	theme	pretreatment	953:964	arg1	conditions					966:975	the pretreatment conditions	949:975	the pretreatment conditions	949:975	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	2	39	from	decrease	395:402	arg1	content					419:425	the protein content	407:425	the protein content induced by the steam explosion	407:456	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	3	40	theme	main	498:501	arg1	modification					517:528	the main compositional modification	494:528	the main compositional modification of polysaccharide	494:546	The monosaccharide analysis showed the main compositional modification of polysaccharide is the increase in the proportion of galacturonic acid, galactose, and arabinose.					
34923399	2	41	from	increase	286:293	arg1	yield					313:317	extraction yield	302:317	extraction yield	302:317	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	2	41	from	increase	286:293	arg1	content					335:341	uronic acid content	323:341	uronic acid content	323:341	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	4	42	theme	FT-IR	685:689	arg1	spectra					702:708	the FT-IR and UV-vis spectra	681:708	spectra	702:708	It is consistent with the discriminant analysis of the FT-IR and UV-vis spectra.					
34923399	5	43	theme	polysaccharide	751:764	arg1	modifications					728:740	These structural modifications	711:740	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP)	711:813	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP) resulted in the significant increases in their antioxidant activities in vitro and in vivo.					
34923399	6	44	from	conditions	966:975	arg1	characteristics					1011:1025	structural characteristics	1000:1025	structural characteristics	1000:1025	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	6	44	from	conditions	966:975	arg1	activities					1043:1052	antioxidant activities	1031:1052	antioxidant activities	1031:1052	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	4	45	with	consistent	636:645	arg1	analysis					669:676	the discriminant analysis	652:676	the discriminant analysis of the FT-IR and UV-vis spectra	652:708	It is consistent with the discriminant analysis of the FT-IR and UV-vis spectra.					
34923399	0	46	dep	pretreatment	16:27	arg1	activities					106:115	its antioxidant activities	90:115	its antioxidant activities	90:115	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.					
34923399	0	46	dep	pretreatment	16:27	arg1	polysaccharide					71:84	Modified polysaccharide	62:84	Modified polysaccharide	62:84	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.					
34923399	2	47	theme	acid	330:333	arg1	content					335:341	uronic acid content	323:341	uronic acid content	323:341	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	6	48	theme	Achyranthis	1057:1067	arg1	ABPS					1103:1106	ABPS	1103:1106	ABPS	1103:1106	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	6	48	theme	Achyranthis	1057:1067	arg1	polysaccharides					1086:1100	Achyranthis bidentatae radix polysaccharides	1057:1100	Achyranthis bidentatae radix polysaccharides (ABPS)	1057:1107	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	5	49	theme	antioxidant	862:872	arg1	activities					874:883	their antioxidant activities	856:883	their antioxidant activities	856:883	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP) resulted in the significant increases in their antioxidant activities in vitro and in vivo.					
34923399	2	50	dep	yield	313:317	arg1	the					298:300	the	298:300	the	298:300	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	0	51	theme	Modified	62:69	arg1	polysaccharide					71:84	Modified polysaccharide	62:84	Modified polysaccharide	62:84	Steam explosion pretreatment of Achyranthis bidentatae radix: Modified polysaccharide and its antioxidant activities.					
34923399	6	52	dep	characteristics	1011:1025	arg1	the					996:998	the	996:998	the	996:998	Strong correlations were observed between the pretreatment conditions and the changes in the structural characteristics and antioxidant activities of Achyranthis bidentatae radix polysaccharides (ABPS).					
34923399	3	53	theme	arabinose	619:627	arg1	proportion					571:580	the proportion	567:580	the proportion of galacturonic acid, galactose, and arabinose	567:627	The monosaccharide analysis showed the main compositional modification of polysaccharide is the increase in the proportion of galacturonic acid, galactose, and arabinose.					
34923399	2	54	theme	steam	442:446	arg1	explosion					448:456	the steam explosion	438:456	the steam explosion	438:456	An increase in the extraction yield and uronic acid content of crude polysaccharide were detected, along with a decrease in the protein content induced by the steam explosion.					
34923399	5	55	theme	crude	745:749	arg1	polysaccharide					751:764	crude polysaccharide	745:764	crude polysaccharide caused by the steam explosion pretreatment (SEP)	745:813	These structural modifications of crude polysaccharide caused by the steam explosion pretreatment (SEP) resulted in the significant increases in their antioxidant activities in vitro and in vivo.					
33861503	0	0	theme	Leucopaxillus	93:105	arg1	Mushroom					84:91	Edible Mushroom Leucopaxillus giganteus	77:115	Edible Mushroom Leucopaxillus giganteus	77:115	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	0	1	from	Characterization	15:30	arg1	Mushroom					84:91	Edible Mushroom Leucopaxillus giganteus	77:115	Edible Mushroom Leucopaxillus giganteus	77:115	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	0	1	from	Characterization	15:30	arg1	Mice					165:168	H22 Tumor-Bearing Mice	147:168	H22 Tumor-Bearing Mice	147:168	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	5	2	theme	mitochondria-mediated	1069:1089	arg1	pathway					1101:1107	mitochondria-mediated apoptotic pathway	1069:1107	mitochondria-mediated apoptotic pathway	1069:1107	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	5	3	theme	phase	1052:1056	arg1	arrest					1058:1063	S phase arrest	1050:1063	S phase arrest	1050:1063	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	6	4	theme	valuable	1136:1143	arg1	agent					1214:1218	an anti-hepatoma agent	1197:1218	an anti-hepatoma agent	1197:1218	Our results could provide valuable information for the potential application of LGP as an anti-hepatoma agent.					
33861503	6	4	theme	valuable	1136:1143	arg1	information					1145:1155	valuable information	1136:1155	valuable information for the potential application of LGP	1136:1192	Our results could provide valuable information for the potential application of LGP as an anti-hepatoma agent.					
33861503	2	5	theme	ion	490:492	arg1	chromatography					494:507	ion chromatography	490:507	ion chromatography	490:507	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	4	6	theme	H22	842:844	arg1	tumors					852:857	H22 solid tumors	842:857	H22 solid tumors	842:857	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	6	7	theme	LGP	1190:1192	arg1	application					1175:1185	the potential application	1161:1185	the potential application of LGP	1161:1192	Our results could provide valuable information for the potential application of LGP as an anti-hepatoma agent.					
33861503	4	8	theme	solid	846:850	arg1	tumors					852:857	H22 solid tumors	842:857	H22 solid tumors	842:857	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	5	9	theme	cell	928:931	arg1	cycle					933:937	cell cycle	928:937	cell cycle	928:937	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	1	10	theme	average	259:265	arg1	weight					277:282	the average molecular weight	255:282	the average molecular weight of 1.78×106 Da	255:297	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	5	11	theme	Annexin	900:906	arg1	staining					918:925	Annexin V-FITC/PI staining	900:925	Annexin V-FITC/PI staining	900:925	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	0	12	from	Polysaccharide	57:70	arg1	Mice					165:168	H22 Tumor-Bearing Mice	147:168	H22 Tumor-Bearing Mice	147:168	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	2	13	theme	2.568 	573:578	arg1	ratio					564:568	a molar ratio	556:568	a molar ratio of 2.568 : 1.209 : 1 : 0.853	556:597	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	5	14	theme	membrane	957:964	arg1	potential					966:974	mitochondrial membrane potential	943:974	mitochondrial membrane potential detection assay	943:990	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	0	15	from	Mice	165:168	arg1	Characterization					15:30	The Structural Characterization	0:30	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.	0:169	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	0	16	theme	Antitumor	125:133	arg1	Activity					135:142	Its Antitumor Activity	121:142	Its Antitumor Activity on H22 Tumor-Bearing Mice	121:168	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	1	17	theme	primary	363:369	arg1	structure					371:379	its primary structure	359:379	its primary structure as well as in vivo antitumor activity	359:417	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	5	18	theme	V-FITC/PI	908:916	arg1	staining					918:925	Annexin V-FITC/PI staining	900:925	Annexin V-FITC/PI staining	900:925	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	1	19	theme	present	178:184	arg1	study					186:190	the present study	174:190	the present study	174:190	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	1	20	theme	molecular	267:275	arg1	weight					277:282	the average molecular weight	255:282	the average molecular weight of 1.78×106 Da	255:297	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	5	21	theme	mitochondrial	943:955	arg1	potential					966:974	mitochondrial membrane potential	943:974	mitochondrial membrane potential detection assay	943:990	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	0	22	theme	Structural	4:13	arg1	Characterization					15:30	The Structural Characterization	0:30	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.	0:169	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	2	23	dep	ratio	564:568	arg1	 1.209 					580:586	 1.209 	580:586	 1.209 	580:586	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	2	23	dep	ratio	564:568	arg1	 0.853					592:597	 0.853	592:597	 0.853	592:597	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	2	23	dep	ratio	564:568	arg1	 1 					588:590	 1 	588:590	 1 	588:590	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	4	24	theme	antitumor	691:699	arg1	experiment					701:710	in vivo antitumor experiment	683:710	in vivo antitumor experiment	683:710	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	0	25	dep	Mushroom	84:91	arg1	giganteus					107:115	Edible Mushroom Leucopaxillus giganteus	77:115	Edible Mushroom Leucopaxillus giganteus	77:115	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	5	26	theme	cells	1040:1044	arg1	apoptosis					1023:1031	apoptosis	1023:1031	apoptosis of H22 cells	1023:1044	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	2	27	theme	monosaccharide	438:451	arg1	composition					453:463	The monosaccharide composition	434:463	The monosaccharide composition of LGP	434:470	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	2	27	theme	monosaccharide	438:451	arg1	galactose					515:523	galactose	515:523	galactose	515:523	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	5	28	theme	S	1050:1050	arg1	arrest					1058:1063	S phase arrest	1050:1063	S phase arrest	1050:1063	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	1	29	theme	novel	195:199	arg1	LGP					245:247	LGP	245:247	LGP	245:247	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	1	29	theme	novel	195:199	arg1	fraction					235:242	a novel cold water-soluble polysaccharide fraction	193:242	a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da	193:297	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	4	30	theme	in vivo	683:689	arg1	experiment					701:710	in vivo antitumor experiment	683:710	in vivo antitumor experiment	683:710	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	6	31	theme	potential	1165:1173	arg1	application					1175:1185	the potential application	1161:1185	the potential application of LGP	1161:1192	Our results could provide valuable information for the potential application of LGP as an anti-hepatoma agent.					
33861503	0	32	theme	Activity	135:142	arg1	Characterization					15:30	The Structural Characterization	0:30	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.	0:169	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	5	33	theme	H22	1036:1038	arg1	cells					1040:1044	H22 cells	1036:1044	H22 cells	1036:1044	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	4	34	theme	experiment	701:710	arg1	results					672:678	The results	668:678	The results of in vivo antitumor experiment	668:710	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	1	35	theme	cold	201:204	arg1	LGP					245:247	LGP	245:247	LGP	245:247	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	1	35	theme	cold	201:204	arg1	fraction					235:242	a novel cold water-soluble polysaccharide fraction	193:242	a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da	193:297	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	0	36	theme	Novel	37:41	arg1	Polysaccharide					57:70	a Novel Water-Soluble Polysaccharide	35:70	a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus	35:115	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	1	37	theme	Da	296:297	arg1	weight					277:282	the average molecular weight	255:282	the average molecular weight of 1.78×106 Da	255:297	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	0	38	theme	Tumor-Bearing	151:163	arg1	Mice					165:168	H22 Tumor-Bearing Mice	147:168	H22 Tumor-Bearing Mice	147:168	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	0	39	from	Mushroom	84:91	arg1	Characterization					15:30	The Structural Characterization	0:30	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.	0:169	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	0	39	from	Mushroom	84:91	arg1	Activity					135:142	Its Antitumor Activity	121:142	Its Antitumor Activity on H22 Tumor-Bearing Mice	121:168	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	0	39	from	Mushroom	84:91	arg1	Polysaccharide					57:70	a Novel Water-Soluble Polysaccharide	35:70	a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus	35:115	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	4	40	theme	excellent	779:787	arg1	activity					799:806	excellent antitumor activity	779:806	excellent antitumor activity	779:806	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	1	41	theme	water-soluble	206:218	arg1	LGP					245:247	LGP	245:247	LGP	245:247	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	1	41	theme	water-soluble	206:218	arg1	fraction					235:242	a novel cold water-soluble polysaccharide fraction	193:242	a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da	193:297	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	0	42	theme	H22	147:149	arg1	Mice					165:168	H22 Tumor-Bearing Mice	147:168	H22 Tumor-Bearing Mice	147:168	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	1	43	theme	in vivo	392:398	arg1	activity					410:417	in vivo antitumor activity	392:417	its primary structure as well as in vivo antitumor activity	359:417	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	1	44	theme	polysaccharide	220:233	arg1	LGP					245:247	LGP	245:247	LGP	245:247	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	1	44	theme	polysaccharide	220:233	arg1	fraction					235:242	a novel cold water-soluble polysaccharide fraction	193:242	a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da	193:297	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	0	45	theme	Polysaccharide	57:70	arg1	Characterization					15:30	The Structural Characterization	0:30	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.	0:169	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	1	46	theme	antitumor	400:408	arg1	activity					410:417	in vivo antitumor activity	392:417	its primary structure as well as in vivo antitumor activity	359:417	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	6	47	theme	anti-hepatoma	1200:1212	arg1	agent					1214:1218	an anti-hepatoma agent	1197:1218	an anti-hepatoma agent	1197:1218	Our results could provide valuable information for the potential application of LGP as an anti-hepatoma agent.					
33861503	6	47	theme	anti-hepatoma	1200:1212	arg1	information					1145:1155	valuable information	1136:1155	valuable information for the potential application of LGP	1136:1192	Our results could provide valuable information for the potential application of LGP as an anti-hepatoma agent.					
33861503	4	48	theme	dose-dependent	864:877	arg1	manner					879:884	a dose-dependent manner	862:884	a dose-dependent manner	862:884	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	0	49	theme	Water-Soluble	43:55	arg1	Polysaccharide					57:70	a Novel Water-Soluble Polysaccharide	35:70	a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus	35:115	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	4	50	theme	tumors	852:857	arg1	proliferation					825:837	the proliferation	821:837	the proliferation of H22 solid tumors	821:857	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	2	51	theme	LGP	468:470	arg1	composition					453:463	The monosaccharide composition	434:463	The monosaccharide composition of LGP	434:470	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	2	51	theme	LGP	468:470	arg1	galactose					515:523	galactose	515:523	galactose	515:523	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	4	52	theme	antitumor	789:797	arg1	activity					799:806	excellent antitumor activity	779:806	excellent antitumor activity	779:806	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	2	53	theme	molar	558:562	arg1	ratio					564:568	a molar ratio	556:568	a molar ratio of 2.568 : 1.209 : 1 : 0.853	556:597	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	2	54	from	galactose	515:523	arg1	ratio					564:568	a molar ratio	556:568	a molar ratio of 2.568 : 1.209 : 1 : 0.853	556:597	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	4	55	theme	immune	760:765	arg1	organs					767:772	immune organs	760:772	immune organs	760:772	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	5	56	theme	potential	966:974	arg1	assay					986:990	mitochondrial membrane potential detection assay	943:990	mitochondrial membrane potential detection assay	943:990	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	2	57	from	glucose	534:540	arg1	ratio					564:568	a molar ratio	556:568	a molar ratio of 2.568 : 1.209 : 1 : 0.853	556:597	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	0	58	theme	Edible	77:82	arg1	Mushroom					84:91	Edible Mushroom Leucopaxillus giganteus	77:115	Edible Mushroom Leucopaxillus giganteus	77:115	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33861503	2	59	from	xylose	526:531	arg1	ratio					564:568	a molar ratio	556:568	a molar ratio of 2.568 : 1.209 : 1 : 0.853	556:597	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	5	60	theme	detection	976:984	arg1	assay					986:990	mitochondrial membrane potential detection assay	943:990	mitochondrial membrane potential detection assay	943:990	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	4	61	contain	has	775:777	arg2	activity					799:806	excellent antitumor activity	779:806	excellent antitumor activity	779:806	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	4	61	contain	has	775:777	arg1	LGP					730:732	LGP	730:732	LGP	730:732	The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose-dependent manner.					
33861503	5	62	theme	apoptotic	1091:1099	arg1	pathway					1101:1107	mitochondria-mediated apoptotic pathway	1069:1107	mitochondria-mediated apoptotic pathway	1069:1107	By analyzing Annexin V-FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria-mediated apoptotic pathway.					
33861503	2	63	from	fucose	546:551	arg1	ratio					564:568	a molar ratio	556:568	a molar ratio of 2.568 : 1.209 : 1 : 0.853	556:597	The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853.					
33861503	1	64	with	fraction	235:242	arg1	weight					277:282	the average molecular weight	255:282	the average molecular weight of 1.78×106 Da	255:297	In the present study, a novel cold water-soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated.					
33861503	0	65	from	Activity	135:142	arg1	Mice					165:168	H22 Tumor-Bearing Mice	147:168	H22 Tumor-Bearing Mice	147:168	The Structural Characterization of a Novel Water-Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor-Bearing Mice.					
33103478	4	0	theme	useful	505:510	arg1	plants					512:517	useful plants	505:517	useful plants	505:517	Somaclones regenerated from callus in vitro can be a source of new raw material in useful plants.					
33103478	0	1	from	in vitro	31:38	arg1	polysaccharides					10:24	Cell wall polysaccharides	0:24	Cell wall polysaccharides from in vitro	0:38	Cell wall polysaccharides from in vitro propagated Cereus hildmannianus K. Schum.					
33103478	7	2	theme	type	929:932	arg1	arabinogalactan					936:950	type I arabinogalactan	929:950	type I arabinogalactan	929:950	The alkaline extraction of in vivo cultivated plant showed the presence of starch, type I arabinogalactan, and arabinoxylan and the somaclones showed type I arabinogalactan and arabinoxylan in both morphologies.					
33103478	3	3	theme	renewable	345:353	arg1	energy					355:360	renewable energy	345:360	renewable energy	345:360	Currently, limited water resources and increasing demand for renewable energy make cacti a biomass source for the production of biofuels.					
33103478	6	4	theme	polysaccharides	750:764	arg1	absence					731:737	the absence	727:737	the absence of soluble polysaccharides as mucilage	727:776	Somaclones aqueous extraction shows the absence of soluble polysaccharides as mucilage.					
33103478	5	5	theme	wild	679:682	arg1	plant					684:688	the wild plant	675:688	the wild plant	675:688	The objective of this work was to determine if the regenerated plants showing two different morphologies present polysaccharide composition different from the wild plant.					
33103478	3	6	theme	biomass	375:381	arg1	source					383:388	a biomass source	373:388	a biomass source for the production of biofuels	373:419	Currently, limited water resources and increasing demand for renewable energy make cacti a biomass source for the production of biofuels.					
33103478	5	7	theme	different	602:610	arg1	morphologies					612:623	two different morphologies	598:623	two different morphologies	598:623	The objective of this work was to determine if the regenerated plants showing two different morphologies present polysaccharide composition different from the wild plant.					
33103478	8	8	located	found	1006:1010	arg1	somaclones					1019:1028	the somaclones	1015:1028	the somaclones	1015:1028	Hemicelluloses found in the somaclones are not different from in vivo cultivated plants, but somaclones not almost biosynthesize mucilage and starch.					
33103478	8	8	located	found	1006:1010	arg2	Hemicelluloses					991:1004	Hemicelluloses	991:1004	Hemicelluloses found in the somaclones	991:1028	Hemicelluloses found in the somaclones are not different from in vivo cultivated plants, but somaclones not almost biosynthesize mucilage and starch.					
33103478	5	9	theme	work	542:545	arg1	objective					524:532	The objective	520:532	The objective of this work	520:545	The objective of this work was to determine if the regenerated plants showing two different morphologies present polysaccharide composition different from the wild plant.					
33103478	8	10	dep	somaclones	1084:1093	arg1	biosynthesize					1106:1118	biosynthesize	1106:1118	somaclones not almost biosynthesize mucilage and starch	1084:1138	Hemicelluloses found in the somaclones are not different from in vivo cultivated plants, but somaclones not almost biosynthesize mucilage and starch.					
33103478	4	11	theme	raw	489:491	arg1	material					493:500	new raw material	485:500	new raw material	485:500	Somaclones regenerated from callus in vitro can be a source of new raw material in useful plants.					
33103478	6	12	theme	soluble	742:748	arg1	polysaccharides					750:764	soluble polysaccharides	742:764	soluble polysaccharides as mucilage	742:776	Somaclones aqueous extraction shows the absence of soluble polysaccharides as mucilage.					
33103478	4	13	theme	new	485:487	arg1	material					493:500	new raw material	485:500	new raw material	485:500	Somaclones regenerated from callus in vitro can be a source of new raw material in useful plants.					
33103478	5	14	theme	polysaccharide	633:646	arg1	composition					648:658	polysaccharide composition	633:658	polysaccharide composition different from the wild plant	633:688	The objective of this work was to determine if the regenerated plants showing two different morphologies present polysaccharide composition different from the wild plant.					
33103478	2	15	theme	physiological	152:164	arg1	adaptation					166:175	morphological and physiological adaptation	134:175	morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species	134:281	Cereus hildmannianus is a cactus exhibiting morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species.					
33103478	8	16	theme	in vivo	1053:1059	arg1	plants					1072:1077	in vivo cultivated plants	1053:1077	in vivo cultivated plants	1053:1077	Hemicelluloses found in the somaclones are not different from in vivo cultivated plants, but somaclones not almost biosynthesize mucilage and starch.					
33103478	0	17	theme	wall	5:8	arg1	polysaccharides					10:24	Cell wall polysaccharides	0:24	Cell wall polysaccharides from in vitro	0:38	Cell wall polysaccharides from in vitro propagated Cereus hildmannianus K. Schum.					
33103478	4	18	theme	material	493:500	arg1	Somaclones					422:431	Somaclones	422:431	Somaclones regenerated from callus in vitro	422:464	Somaclones regenerated from callus in vitro can be a source of new raw material in useful plants.					
33103478	4	18	theme	material	493:500	arg1	source					475:480	a source	473:480	a source of new raw material in useful plants	473:517	Somaclones regenerated from callus in vitro can be a source of new raw material in useful plants.					
33103478	5	19	theme	different	660:668	arg1	composition					648:658	polysaccharide composition	633:658	polysaccharide composition different from the wild plant	633:688	The objective of this work was to determine if the regenerated plants showing two different morphologies present polysaccharide composition different from the wild plant.					
33103478	5	20	theme	regenerated	571:581	arg1	plants					583:588	the regenerated plants	567:588	the regenerated plants showing two different morphologies	567:623	The objective of this work was to determine if the regenerated plants showing two different morphologies present polysaccharide composition different from the wild plant.					
33103478	0	21	theme	Cell	0:3	arg1	polysaccharides					10:24	Cell wall polysaccharides	0:24	Cell wall polysaccharides from in vitro	0:38	Cell wall polysaccharides from in vitro propagated Cereus hildmannianus K. Schum.					
33103478	7	22	theme	I	867:867	arg1	arabinogalactan					869:883	type I arabinogalactan	862:883	type I arabinogalactan	862:883	The alkaline extraction of in vivo cultivated plant showed the presence of starch, type I arabinogalactan, and arabinoxylan and the somaclones showed type I arabinogalactan and arabinoxylan in both morphologies.					
33103478	7	23	theme	starch	854:859	arg1	presence					842:849	the presence	838:849	the presence of starch, type I arabinogalactan, and arabinoxylan	838:901	The alkaline extraction of in vivo cultivated plant showed the presence of starch, type I arabinogalactan, and arabinoxylan and the somaclones showed type I arabinogalactan and arabinoxylan in both morphologies.					
33103478	3	24	theme	limited	295:301	arg1	resources					309:317	limited water resources	295:317	limited water resources	295:317	Currently, limited water resources and increasing demand for renewable energy make cacti a biomass source for the production of biofuels.					
33103478	8	25	theme	cultivated	1061:1070	arg1	plants					1072:1077	in vivo cultivated plants	1053:1077	in vivo cultivated plants	1053:1077	Hemicelluloses found in the somaclones are not different from in vivo cultivated plants, but somaclones not almost biosynthesize mucilage and starch.					
33103478	0	26	dep	Schum	75:79	arg1	hildmannianus					58:70	hildmannianus	58:70	hildmannianus	58:70	Cell wall polysaccharides from in vitro propagated Cereus hildmannianus K. Schum.					
33103478	7	27	theme	arabinogalactan	869:883	arg1	presence					842:849	the presence	838:849	the presence of starch, type I arabinogalactan, and arabinoxylan	838:901	The alkaline extraction of in vivo cultivated plant showed the presence of starch, type I arabinogalactan, and arabinoxylan and the somaclones showed type I arabinogalactan and arabinoxylan in both morphologies.					
33103478	3	28	theme	water	303:307	arg1	resources					309:317	limited water resources	295:317	limited water resources	295:317	Currently, limited water resources and increasing demand for renewable energy make cacti a biomass source for the production of biofuels.					
33103478	7	29	theme	type	862:865	arg1	arabinogalactan					869:883	type I arabinogalactan	862:883	type I arabinogalactan	862:883	The alkaline extraction of in vivo cultivated plant showed the presence of starch, type I arabinogalactan, and arabinoxylan and the somaclones showed type I arabinogalactan and arabinoxylan in both morphologies.					
33103478	6	30	theme	aqueous	702:708	arg1	extraction					710:719	Somaclones aqueous extraction	691:719	Somaclones aqueous extraction	691:719	Somaclones aqueous extraction shows the absence of soluble polysaccharides as mucilage.					
33103478	6	31	theme	Somaclones	691:700	arg1	extraction					710:719	Somaclones aqueous extraction	691:719	Somaclones aqueous extraction	691:719	Somaclones aqueous extraction shows the absence of soluble polysaccharides as mucilage.					
33103478	5	32	from	plant	684:688	arg1	different					660:668	different	660:668	different	660:668	The objective of this work was to determine if the regenerated plants showing two different morphologies present polysaccharide composition different from the wild plant.					
33103478	2	33	theme	cladodes	184:191	arg1	adaptation					166:175	morphological and physiological adaptation	134:175	morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species	134:281	Cereus hildmannianus is a cactus exhibiting morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species.					
33103478	2	34	theme	unfavourable	247:258	arg1	conditions					236:245	climatic and soil conditions	218:245	conditions	236:245	Cereus hildmannianus is a cactus exhibiting morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species.					
33103478	7	35	theme	arabinoxylan	890:901	arg1	presence					842:849	the presence	838:849	the presence of starch, type I arabinogalactan, and arabinoxylan	838:901	The alkaline extraction of in vivo cultivated plant showed the presence of starch, type I arabinogalactan, and arabinoxylan and the somaclones showed type I arabinogalactan and arabinoxylan in both morphologies.					
33103478	3	36	theme	biofuels	412:419	arg1	production					398:407	the production	394:407	the production of biofuels	394:419	Currently, limited water resources and increasing demand for renewable energy make cacti a biomass source for the production of biofuels.					
33103478	7	37	theme	plant	825:829	arg1	extraction					792:801	The alkaline extraction	779:801	The alkaline extraction of in vivo cultivated plant	779:829	The alkaline extraction of in vivo cultivated plant showed the presence of starch, type I arabinogalactan, and arabinoxylan and the somaclones showed type I arabinogalactan and arabinoxylan in both morphologies.					
33103478	3	38	theme	increasing	323:332	arg1	demand					334:339	increasing demand	323:339	increasing demand for renewable energy	323:360	Currently, limited water resources and increasing demand for renewable energy make cacti a biomass source for the production of biofuels.					
33103478	4	39	from	source	475:480	arg1	plants					512:517	useful plants	505:517	useful plants	505:517	Somaclones regenerated from callus in vitro can be a source of new raw material in useful plants.					
33103478	4	40	theme	callus	450:455	arg1	in vitro					457:464	callus in vitro	450:464	callus in vitro	450:464	Somaclones regenerated from callus in vitro can be a source of new raw material in useful plants.					
33103478	2	41	theme	soil	231:234	arg1	conditions					236:245	climatic and soil conditions	218:245	conditions	236:245	Cereus hildmannianus is a cactus exhibiting morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species.					
33103478	7	42	theme	cultivated	814:823	arg1	plant					825:829	in vivo cultivated plant	806:829	in vivo cultivated plant	806:829	The alkaline extraction of in vivo cultivated plant showed the presence of starch, type I arabinogalactan, and arabinoxylan and the somaclones showed type I arabinogalactan and arabinoxylan in both morphologies.					
33103478	2	43	theme	climatic	218:225	arg1	conditions					236:245	climatic and soil conditions	218:245	conditions	236:245	Cereus hildmannianus is a cactus exhibiting morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species.					
33103478	7	44	theme	I	934:934	arg1	arabinogalactan					936:950	type I arabinogalactan	929:950	type I arabinogalactan	929:950	The alkaline extraction of in vivo cultivated plant showed the presence of starch, type I arabinogalactan, and arabinoxylan and the somaclones showed type I arabinogalactan and arabinoxylan in both morphologies.					
33103478	2	45	theme	plant	269:273	arg1	species					275:281	many plant species	264:281	many plant species	264:281	Cereus hildmannianus is a cactus exhibiting morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species.					
33103478	8	46	from	plants	1072:1077	arg1	different					1038:1046	different	1038:1046	different	1038:1046	Hemicelluloses found in the somaclones are not different from in vivo cultivated plants, but somaclones not almost biosynthesize mucilage and starch.					
33103478	2	47	theme	morphological	134:146	arg1	adaptation					166:175	morphological and physiological adaptation	134:175	morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species	134:281	Cereus hildmannianus is a cactus exhibiting morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species.					
33103478	2	48	theme	many	264:267	arg1	species					275:281	many plant species	264:281	many plant species	264:281	Cereus hildmannianus is a cactus exhibiting morphological and physiological adaptation of its cladodes which ensuring growth in climatic and soil conditions unfavourable for many plant species.					
33103478	7	49	theme	alkaline	783:790	arg1	extraction					792:801	The alkaline extraction	779:801	The alkaline extraction of in vivo cultivated plant	779:829	The alkaline extraction of in vivo cultivated plant showed the presence of starch, type I arabinogalactan, and arabinoxylan and the somaclones showed type I arabinogalactan and arabinoxylan in both morphologies.					
34885697	0	0	theme	Polysaccharide	84:97	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata.					
34885697	0	0	theme	Polysaccharide	84:97	arg1	Characterization					25:40	Structural Characterization	14:40	Structural Characterization	14:40	Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata.					
34885697	0	0	theme	Polysaccharide	84:97	arg1	Effects					65:71	Anti-Inflammatory Effects	47:71	Anti-Inflammatory Effects	47:71	Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata.					
34885697	6	1	theme	scanning	896:903	arg1	SEM					926:928	SEM	926:928	SEM	926:928	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	6	1	theme	scanning	896:903	arg1	microscopy					914:923	scanning electron microscopy	896:923	scanning electron microscopy (SEM)	896:929	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	7	2	from	secretion	1153:1161	arg1	cells					1199:1203	RAW264.7 cells	1190:1203	RAW264.7 cells	1190:1203	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	1	3	theme	structural	168:177	arg1	characteristics					179:193	structural characteristics	168:193	structural characteristics	168:193	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	0	4	theme	Novel	78:82	arg1	Polysaccharide					84:97	a Novel Polysaccharide	76:97	a Novel Polysaccharide	76:97	Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata.					
34885697	5	5	theme	-β-Glcp-	771:778	arg1	-β-Glcp-					808:815	→4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-	727:815	→4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-	727:815	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	4	6	theme	composition	553:563	arg1	analysis					565:572	HPGPC and monosaccharide composition analysis	528:572	analysis	565:572	HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc.					
34885697	7	7	theme	RAW264.7	1190:1197	arg1	cells					1199:1203	RAW264.7 cells	1190:1203	RAW264.7 cells	1190:1203	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	6	8	theme	force	942:946	arg1	AFM					960:962	AFM	960:962	AFM	960:962	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	6	8	theme	force	942:946	arg1	microscopy					948:957	atomic force microscopy	935:957	atomic force microscopy (AFM)	935:963	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	6	9	theme	Morphological	867:879	arg1	analysis					881:888	Morphological analysis	867:888	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM)	867:963	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	7	10	from	extent	1227:1232	arg1	mice					1268:1271	mice	1268:1271	mice	1268:1271	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	6	11	theme	polysaccharide	1049:1062	arg1	molecules					1036:1044	the sugar chain molecules	1020:1044	the sugar chain molecules of polysaccharide	1020:1062	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	1	12	theme	traditional	299:309	arg1	plant					329:333	a traditional Chinese medicinal plant	297:333	a traditional Chinese medicinal plant	297:333	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	1	12	theme	traditional	299:309	arg1	which					288:292	which	288:292	which	288:292	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	5	13	theme	chemical	695:702	arg1	structure					704:712	the possible chemical structure	682:712	the possible chemical structure	682:712	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	2	14	theme	Sephadex	458:465	arg1	column					472:477	a DEAE cellulose-52 and Sephadex G-75 column	434:477	column	472:477	O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column.					
34885697	6	15	contain	had	984:986	arg1	OFP					980:982	OFP	980:982	OFP	980:982	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	6	15	contain	had	984:986	arg2	structure					1005:1013	a multi-branched structure	988:1013	a multi-branched structure	988:1013	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	2	16	theme	DEAE	436:439	arg1	cellulose-52					441:452	a DEAE cellulose-52 and Sephadex G-75 column	434:477	cellulose-52	441:452	O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column.					
34885697	0	17	theme	Orostachys	113:122	arg1	fimbriata					124:132	Orostachys fimbriata	113:132	Orostachys fimbriata	113:132	Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata.					
34885697	4	18	theme	HPGPC	528:532	arg1	analysis					565:572	HPGPC and monosaccharide composition analysis	528:572	analysis	565:572	HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc.					
34885697	7	19	theme	anti-inflammatory	1110:1126	arg1	activity					1128:1135	anti-inflammatory activity	1110:1135	anti-inflammatory activity	1110:1135	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	3	20	theme	molecular	484:492	arg1	kDa					523:525	6.2 kDa	519:525	6.2 kDa	519:525	The molecular weight was determined as 6.2 kDa.					
34885697	3	20	theme	molecular	484:492	arg1	weight					494:499	The molecular weight	480:499	The molecular weight	480:499	The molecular weight was determined as 6.2 kDa.					
34885697	6	21	dep	appeared	1064:1071	arg1	aggregated					1073:1082	aggregated	1073:1082	appeared aggregated	1064:1082	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	4	22	theme	only	623:626	arg1	Glc					628:630	only Glc	623:630	only Glc	623:630	HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc.					
34885697	6	23	theme	sugar	1024:1028	arg1	molecules					1036:1044	the sugar chain molecules	1020:1044	the sugar chain molecules of polysaccharide	1020:1062	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	1	24	theme	Chinese	311:317	arg1	plant					329:333	a traditional Chinese medicinal plant	297:333	a traditional Chinese medicinal plant	297:333	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	1	24	theme	Chinese	311:317	arg1	which					288:292	which	288:292	which	288:292	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	5	25	theme	spectral	652:659	arg1	analysis					661:668	spectral analysis	652:668	spectral analysis	652:668	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	1	26	dep	characteristics	179:193	arg1	the					164:166	the	164:166	the	164:166	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	7	27	dep	exhibit	1102:1108	arg1	reducing					1140:1147	reducing	1140:1147	reducing the secretion of inflammatory factors in RAW264.7 cells	1140:1203	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	7	27	dep	exhibit	1102:1108	arg1	decreasing					1212:1221	decreasing	1212:1221	decreasing the extent of xylene-induced ear swelling in mice	1212:1271	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	1	28	theme	medicinal	319:327	arg1	plant					329:333	a traditional Chinese medicinal plant	297:333	a traditional Chinese medicinal plant	297:333	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	1	28	theme	medicinal	319:327	arg1	which					288:292	which	288:292	which	288:292	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	5	29	theme	possible	686:693	arg1	structure					704:712	the possible chemical structure	682:712	the possible chemical structure	682:712	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	2	30	theme	fimbriata	339:347	arg1	OFP					365:367	OFP	365:367	OFP	365:367	O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column.					
34885697	2	30	theme	fimbriata	339:347	arg1	polysaccharide					349:362	O. fimbriata polysaccharide	336:362	O. fimbriata polysaccharide (OFP)	336:368	O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column.					
34885697	6	31	theme	chain	1030:1034	arg1	molecules					1036:1044	the sugar chain molecules	1020:1044	the sugar chain molecules of polysaccharide	1020:1062	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	2	32	theme	G-75	467:470	arg1	column					472:477	a DEAE cellulose-52 and Sephadex G-75 column	434:477	column	472:477	O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column.					
34885697	0	33	theme	Structural	14:23	arg1	Characterization					25:40	Structural Characterization	14:40	Structural Characterization	14:40	Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata.					
34885697	6	34	theme	atomic	935:940	arg1	AFM					960:962	AFM	960:962	AFM	960:962	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	6	34	theme	atomic	935:940	arg1	microscopy					948:957	atomic force microscopy	935:957	atomic force microscopy (AFM)	935:963	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	5	35	from	1→	780:781	arg1	chain					795:799	the main chain	786:799	the main chain	786:799	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	2	36	theme	O.	336:337	arg1	OFP					365:367	OFP	365:367	OFP	365:367	O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column.					
34885697	2	36	theme	O.	336:337	arg1	polysaccharide					349:362	O. fimbriata polysaccharide	336:362	O. fimbriata polysaccharide (OFP)	336:368	O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column.					
34885697	1	37	theme	novel	231:235	arg1	polysaccharide					237:250	a novel polysaccharide	229:250	a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant	229:333	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	5	38	dep	consisted	714:722	arg1	1→					845:846	1→	845:846	1→ in the side chain	845:864	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	5	38	dep	consisted	714:722	arg1	β-Glcp-					837:843	β-Glcp-	837:843	β-Glcp-	837:843	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	7	39	from	swelling	1256:1263	arg1	mice					1268:1271	mice	1268:1271	mice	1268:1271	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	5	40	theme	→4,6	766:769	arg1	1→					739:740	1→	739:740	1→	739:740	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	5	40	theme	→4,6	766:769	arg1	quantity					754:761	a small quantity	746:761	a small quantity of →4,6	746:769	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	7	41	theme	inflammatory	1166:1177	arg1	factors					1179:1185	inflammatory factors	1166:1185	inflammatory factors	1166:1185	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	1	42	theme	polysaccharide	237:250	arg1	activity					217:224	anti-inflammatory activity	199:224	anti-inflammatory activity	199:224	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	1	42	theme	polysaccharide	237:250	arg1	characteristics					179:193	structural characteristics	168:193	structural characteristics	168:193	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	7	43	from	mice	1268:1271	arg1	extent					1227:1232	the extent	1223:1232	the extent of xylene-induced ear swelling in mice	1223:1271	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	6	44	theme	multi-branched	990:1003	arg1	structure					1005:1013	a multi-branched structure	988:1013	a multi-branched structure	988:1013	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	7	45	theme	factors	1179:1185	arg1	secretion					1153:1161	the secretion	1149:1161	the secretion of inflammatory factors in RAW264.7 cells	1149:1203	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	1	46	dep	Orostachys	266:275	arg1	fimbriata					277:285	Orostachys fimbriata, which is a traditional Chinese medicinal plant	266:333	fimbriata	277:285	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	1	46	dep	Orostachys	266:275	arg1	plant					329:333	a traditional Chinese medicinal plant	297:333	a traditional Chinese medicinal plant	297:333	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	1	46	dep	Orostachys	266:275	arg1	which					288:292	which	288:292	which	288:292	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	7	47	theme	swelling	1256:1263	arg1	extent					1227:1232	the extent	1223:1232	the extent of xylene-induced ear swelling in mice	1223:1271	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	0	48	theme	Anti-Inflammatory	47:63	arg1	Effects					65:71	Anti-Inflammatory Effects	47:71	Anti-Inflammatory Effects	47:71	Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata.					
34885697	6	49	theme	electron	905:912	arg1	SEM					926:928	SEM	926:928	SEM	926:928	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	6	49	theme	electron	905:912	arg1	microscopy					914:923	scanning electron microscopy	896:923	scanning electron microscopy (SEM)	896:929	Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated.					
34885697	5	50	dep	1→	845:846	arg1	1→					829:830	1→	829:830	1→	829:830	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	5	50	dep	1→	845:846	arg1	α-Glcp-					821:827	α-Glcp-	821:827	α-Glcp-	821:827	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	5	50	dep	1→	845:846	arg1	1→					817:818	1→	817:818	1→	817:818	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	4	51	theme	homogeneous	585:595	arg1	polysaccharide					597:610	a homogeneous polysaccharide	583:610	a homogeneous polysaccharide containing only Glc	583:630	HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc.					
34885697	5	52	dep	-β-Glcp-	771:778	arg1	1→					780:781	1→	780:781	1→	780:781	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	5	52	dep	-β-Glcp-	771:778	arg1	1→					739:740	1→	739:740	1→	739:740	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	5	52	dep	-β-Glcp-	771:778	arg1	→6					805:806	→6	805:806	→6	805:806	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	5	52	dep	-β-Glcp-	771:778	arg1	quantity					754:761	a small quantity	746:761	a small quantity of →4,6	746:769	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	1	53	theme	present	139:145	arg1	study					147:151	The present study	135:151	The present study	135:151	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	4	54	contain	containing	612:621	arg1	polysaccharide					597:610	a homogeneous polysaccharide	583:610	a homogeneous polysaccharide containing only Glc	583:630	HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc.					
34885697	4	54	contain	containing	612:621	arg2	Glc					628:630	only Glc	623:630	only Glc	623:630	HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc.					
34885697	5	55	theme	small	748:752	arg1	quantity					754:761	a small quantity	746:761	a small quantity of →4,6	746:769	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	1	56	attach	isolated	252:259	arg2	polysaccharide					237:250	a novel polysaccharide	229:250	a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant	229:333	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	1	56	attach	isolated	252:259	arg1	Orostachys					266:275	Orostachys	266:275	Orostachys	266:275	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	7	57	theme	ear	1252:1254	arg1	swelling					1256:1263	xylene-induced ear swelling	1237:1263	xylene-induced ear swelling in mice	1237:1271	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	7	58	theme	xylene-induced	1237:1250	arg1	swelling					1256:1263	xylene-induced ear swelling	1237:1263	xylene-induced ear swelling in mice	1237:1271	OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.					
34885697	5	59	theme	side	855:858	arg1	chain					860:864	the side chain	851:864	the side chain	851:864	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	1	60	theme	anti-inflammatory	199:215	arg1	activity					217:224	anti-inflammatory activity	199:224	anti-inflammatory activity	199:224	The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant.					
34885697	5	61	theme	main	790:793	arg1	chain					795:799	the main chain	786:799	the main chain	786:799	Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain.					
34885697	4	62	theme	monosaccharide	538:551	arg1	analysis					565:572	HPGPC and monosaccharide composition analysis	528:572	analysis	565:572	HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc.					
31594266	0	0	theme	composites	93:102	arg1	properties					48:57	surface mechanical properties	29:57	surface mechanical properties	29:57	Evaluating antibacterial and surface mechanical properties of chitosan modified dental resin composites.					
31594266	0	0	theme	composites	93:102	arg1	antibacterial					11:23	antibacterial	11:23	antibacterial	11:23	Evaluating antibacterial and surface mechanical properties of chitosan modified dental resin composites.					
31594266	8	1	contain	had	1273:1275	arg1	RBCs					1251:1254	The flowable RBCs	1238:1254	The flowable RBCs containing 1% CS	1238:1271	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	8	1	contain	had	1273:1275	arg2	VH					1298:1299	significantly higher VH	1277:1299	significantly higher VH	1277:1299	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	9	2	theme	0.50	1400:1403	arg1	%					1404:1404	%	1404:1404	%	1404:1404	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	1	3	theme	dental	176:181	arg1	materials					205:213	dental restorative composite materials	176:213	dental restorative composite materials	176:213	BACKGROUND The antibacterial properties are beneficial and desired for dental restorative composite materials.					
31594266	4	4	theme	microhybrid	629:639	arg1	RBCs					654:657	microhybrid and flowable RBCs	629:657	microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria	629:746	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	0	5	theme	resin	87:91	arg1	composites					93:102	chitosan modified dental resin composites	62:102	chitosan modified dental resin composites	62:102	Evaluating antibacterial and surface mechanical properties of chitosan modified dental resin composites.					
31594266	6	6	theme	Actinomyces	1063:1073	arg1	viscous					1075:1081	Actinomyces viscous	1063:1081	Actinomyces viscous	1063:1081	RESULTS The results revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous.					
31594266	9	7	theme	microhybrid	1468:1478	arg1	group					1484:1488	experimental microhybrid RBC group	1455:1488	experimental microhybrid RBC group containing 1% CS	1455:1505	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	1	8	theme	restorative	183:193	arg1	materials					205:213	dental restorative composite materials	176:213	dental restorative composite materials	176:213	BACKGROUND The antibacterial properties are beneficial and desired for dental restorative composite materials.					
31594266	1	9	dep	BACKGROUND	105:114	arg1	beneficial					149:158	beneficial	149:158	beneficial	149:158	BACKGROUND The antibacterial properties are beneficial and desired for dental restorative composite materials.					
31594266	1	9	dep	BACKGROUND	105:114	arg1	properties					134:143	The antibacterial properties	116:143	The antibacterial properties	116:143	BACKGROUND The antibacterial properties are beneficial and desired for dental restorative composite materials.					
31594266	1	9	dep	BACKGROUND	105:114	arg1	desired					164:170	desired	164:170	desired	164:170	BACKGROUND The antibacterial properties are beneficial and desired for dental restorative composite materials.					
31594266	6	10	dep	RESULTS	909:915	arg1	revealed					929:936	revealed	929:936	revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous	929:1081	RESULTS The results revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous.					
31594266	3	11	theme	study	412:416	arg1	aim					393:395	The aim	389:395	The aim of the current study	389:416	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	4	12	dep	%	691:691	arg1	1					690:690	1	690:690	1	690:690	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	4	12	dep	%	691:691	arg1	0.5					682:684	0.5	682:684	0.5	682:684	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	7	13	theme	colony	1122:1127	arg1	count					1148:1152	colony forming unit (CFU) count	1122:1152	colony forming unit (CFU) count of Actinomyces viscous	1122:1175	The direct contact test revealed that colony forming unit (CFU) count of Actinomyces viscous was comparable among the experimental and control materials.					
31594266	8	14	theme	RBC	1353:1355	arg1	groups					1357:1362	other experimental flowable RBC groups	1325:1362	other experimental flowable RBC groups	1325:1362	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	4	15	theme	flowable	645:652	arg1	RBCs					654:657	microhybrid and flowable RBCs	629:657	microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria	629:746	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	4	16	theme	diffusion	772:780	arg1	test					782:785	agar diffusion test and direct contact methods	767:812	test	782:785	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	3	17	theme	novel	550:554	arg1	CS					586:587	CS	586:587	CS	586:587	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	3	17	theme	novel	550:554	arg1	chitosan					576:583	novel antimicrobial agent chitosan	550:583	novel antimicrobial agent chitosan (CS)	550:588	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	9	18	theme	RBC	1480:1482	arg1	group					1484:1488	experimental microhybrid RBC group	1455:1488	experimental microhybrid RBC group containing 1% CS	1455:1505	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	8	19	theme	experimental	1331:1342	arg1	groups					1357:1362	other experimental flowable RBC groups	1325:1362	other experimental flowable RBC groups	1325:1362	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	9	20	theme	%	1502:1502	arg1	CS					1504:1505	1% CS	1501:1505	1% CS	1501:1505	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	8	21	contain	containing	1256:1265	arg2	CS					1270:1271	1% CS	1267:1271	1% CS	1267:1271	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	8	21	contain	containing	1256:1265	arg1	RBCs					1251:1254	The flowable RBCs	1238:1254	The flowable RBCs containing 1% CS	1238:1271	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	5	22	theme	RBCs	856:859	arg1	hardness					819:826	The hardness	815:826	The hardness of control and experimental RBCs	815:859	The hardness of control and experimental RBCs was determined by Vickers hardness (VH) tester.					
31594266	2	23	theme	mechanical	322:331	arg1	properties					333:342	their physical and mechanical properties	303:342	their physical and mechanical properties	303:342	The incorporation of various antimicrobial agents into resin composites may compromise their physical and mechanical properties hence limiting their applications.					
31594266	5	24	theme	hardness	887:894	arg1	tester					901:906	Vickers hardness (VH) tester	879:906	Vickers hardness (VH) tester	879:906	The hardness of control and experimental RBCs was determined by Vickers hardness (VH) tester.					
31594266	1	25	theme	composite	195:203	arg1	materials					205:213	dental restorative composite materials	176:213	dental restorative composite materials	176:213	BACKGROUND The antibacterial properties are beneficial and desired for dental restorative composite materials.					
31594266	3	26	theme	current	404:410	arg1	study					412:416	the current study	400:416	the current study	400:416	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	6	27	theme	growth	1018:1023	arg1	zone					1036:1039	growth inhibition zone	1018:1039	growth inhibition zone	1018:1039	RESULTS The results revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous.					
31594266	7	28	theme	direct	1088:1093	arg1	test					1103:1106	The direct contact test	1084:1106	The direct contact test	1084:1106	The direct contact test revealed that colony forming unit (CFU) count of Actinomyces viscous was comparable among the experimental and control materials.					
31594266	4	29	dep	chitosan	697:704	arg1	%					691:691	%	691:691	%	691:691	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	3	30	theme	flowable	496:503	arg1	RBCs					529:532	RBCs	529:532	RBCs	529:532	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	3	30	theme	flowable	496:503	arg1	composites					517:526	resin based composites	505:526	microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS)	480:588	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	4	31	dep	METHODS	591:597	arg1	explored					752:759	explored	752:759	was explored using agar diffusion test and direct contact methods	748:812	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	7	32	theme	contact	1095:1101	arg1	test					1103:1106	The direct contact test	1084:1106	The direct contact test	1084:1106	The direct contact test revealed that colony forming unit (CFU) count of Actinomyces viscous was comparable among the experimental and control materials.					
31594266	4	33	theme	w/w	693:695	arg1	CS					707:708	CS	707:708	CS	707:708	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	4	33	theme	w/w	693:695	arg1	chitosan					697:704	0, 0.25, 0.5 and 1% w/w chitosan	673:704	0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria	673:746	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	3	34	theme	antibacterial	437:449	arg1	activity					451:458	the antibacterial activity	433:458	the antibacterial activity	433:458	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	9	35	theme	higher	1433:1438	arg1	VH					1440:1441	significantly higher VH	1419:1441	significantly higher VH	1419:1441	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	8	36	theme	%	1268:1268	arg1	CS					1270:1271	1% CS	1267:1271	1% CS	1267:1271	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	3	37	theme	microhybrid	480:490	arg1	RBCs					529:532	RBCs	529:532	RBCs	529:532	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	3	37	theme	microhybrid	480:490	arg1	composites					517:526	resin based composites	505:526	microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS)	480:588	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	0	38	theme	surface	29:35	arg1	properties					48:57	surface mechanical properties	29:57	surface mechanical properties	29:57	Evaluating antibacterial and surface mechanical properties of chitosan modified dental resin composites.					
31594266	6	39	theme	microhybrid	981:991	arg1	RBCs					993:996	control and experimental flowable and microhybrid RBCs	943:996	control and experimental flowable and microhybrid RBCs	943:996	RESULTS The results revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous.					
31594266	3	40	dep	OBJECTIVE	379:387	arg1	is					418:419	is	418:419	is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS)	418:588	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	4	41	theme	agar	767:770	arg1	test					782:785	agar diffusion test and direct contact methods	767:812	test	782:785	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	9	42	theme	experimental	1455:1466	arg1	group					1484:1488	experimental microhybrid RBC group	1455:1488	experimental microhybrid RBC group containing 1% CS	1455:1505	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	3	43	theme	composites	517:526	arg1	activity					451:458	the antibacterial activity	433:458	the antibacterial activity	433:458	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	3	43	theme	composites	517:526	arg1	hardness					468:475	the hardness	464:475	the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS)	464:588	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	7	44	theme	viscous	1169:1175	arg1	count					1148:1152	colony forming unit (CFU) count	1122:1152	colony forming unit (CFU) count of Actinomyces viscous	1122:1175	The direct contact test revealed that colony forming unit (CFU) count of Actinomyces viscous was comparable among the experimental and control materials.					
31594266	4	45	theme	direct	791:796	arg1	methods					806:812	agar diffusion test and direct contact methods	767:812	methods	806:812	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	1	46	theme	antibacterial	120:132	arg1	beneficial					149:158	beneficial	149:158	beneficial	149:158	BACKGROUND The antibacterial properties are beneficial and desired for dental restorative composite materials.					
31594266	1	46	theme	antibacterial	120:132	arg1	properties					134:143	The antibacterial properties	116:143	The antibacterial properties	116:143	BACKGROUND The antibacterial properties are beneficial and desired for dental restorative composite materials.					
31594266	5	47	theme	control	831:837	arg1	RBCs					856:859	control and experimental RBCs	831:859	control and experimental RBCs	831:859	The hardness of control and experimental RBCs was determined by Vickers hardness (VH) tester.					
31594266	4	48	theme	antibacterial	603:615	arg1	activity					617:624	The antibacterial activity	599:624	The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria	599:746	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	6	49	theme	flowable	968:975	arg1	RBCs					993:996	control and experimental flowable and microhybrid RBCs	943:996	control and experimental flowable and microhybrid RBCs	943:996	RESULTS The results revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous.					
31594266	6	50	theme	viscous	1075:1081	arg1	growth					1053:1058	the lawn growth	1044:1058	the lawn growth of Actinomyces viscous	1044:1081	RESULTS The results revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous.					
31594266	3	51	theme	resin	505:509	arg1	RBCs					529:532	RBCs	529:532	RBCs	529:532	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	3	51	theme	resin	505:509	arg1	composites					517:526	resin based composites	505:526	microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS)	480:588	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	0	52	theme	mechanical	37:46	arg1	properties					48:57	surface mechanical properties	29:57	surface mechanical properties	29:57	Evaluating antibacterial and surface mechanical properties of chitosan modified dental resin composites.					
31594266	6	53	theme	experimental	955:966	arg1	RBCs					993:996	control and experimental flowable and microhybrid RBCs	943:996	control and experimental flowable and microhybrid RBCs	943:996	RESULTS The results revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous.					
31594266	9	54	theme	1	1501:1501	arg1	%					1502:1502	%	1502:1502	%	1502:1502	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	2	55	theme	resin	271:275	arg1	composites					277:286	resin composites	271:286	resin composites	271:286	The incorporation of various antimicrobial agents into resin composites may compromise their physical and mechanical properties hence limiting their applications.					
31594266	8	56	theme	other	1325:1329	arg1	groups					1357:1362	other experimental flowable RBC groups	1325:1362	other experimental flowable RBC groups	1325:1362	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	3	57	theme	based	511:515	arg1	RBCs					529:532	RBCs	529:532	RBCs	529:532	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	3	57	theme	based	511:515	arg1	composites					517:526	resin based composites	505:526	microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS)	480:588	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	0	58	theme	chitosan	62:69	arg1	composites					93:102	chitosan modified dental resin composites	62:102	chitosan modified dental resin composites	62:102	Evaluating antibacterial and surface mechanical properties of chitosan modified dental resin composites.					
31594266	9	59	theme	microhybrid	1369:1379	arg1	RBCs					1381:1384	The microhybrid RBCs	1365:1384	The microhybrid RBCs consisting of 0.50% CS	1365:1407	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	9	60	contain	containing	1490:1499	arg1	group					1484:1488	experimental microhybrid RBC group	1455:1488	experimental microhybrid RBC group containing 1% CS	1455:1505	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	9	60	contain	containing	1490:1499	arg2	CS					1504:1505	1% CS	1501:1505	1% CS	1501:1505	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	4	61	theme	Actinomyces	719:729	arg1	bacteria					739:746	Actinomyces viscous bacteria	719:746	Actinomyces viscous bacteria	719:746	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	6	62	theme	control	943:949	arg1	RBCs					993:996	control and experimental flowable and microhybrid RBCs	943:996	control and experimental flowable and microhybrid RBCs	943:996	RESULTS The results revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous.					
31594266	2	63	theme	antimicrobial	245:257	arg1	agents					259:264	various antimicrobial agents	237:264	various antimicrobial agents	237:264	The incorporation of various antimicrobial agents into resin composites may compromise their physical and mechanical properties hence limiting their applications.					
31594266	2	64	theme	agents	259:264	arg1	incorporation					220:232	The incorporation	216:232	The incorporation of various antimicrobial agents into resin composites	216:286	The incorporation of various antimicrobial agents into resin composites may compromise their physical and mechanical properties hence limiting their applications.					
31594266	3	65	theme	antimicrobial	556:568	arg1	CS					586:587	CS	586:587	CS	586:587	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	3	65	theme	antimicrobial	556:568	arg1	chitosan					576:583	novel antimicrobial agent chitosan	550:583	novel antimicrobial agent chitosan (CS)	550:588	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	8	66	theme	higher	1291:1296	arg1	VH					1298:1299	significantly higher VH	1277:1299	significantly higher VH	1277:1299	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	7	67	theme	Actinomyces	1157:1167	arg1	viscous					1169:1175	Actinomyces viscous	1157:1175	Actinomyces viscous	1157:1175	The direct contact test revealed that colony forming unit (CFU) count of Actinomyces viscous was comparable among the experimental and control materials.					
31594266	7	68	dep	experimental	1202:1213	arg1	materials					1227:1235	materials	1227:1235	materials	1227:1235	The direct contact test revealed that colony forming unit (CFU) count of Actinomyces viscous was comparable among the experimental and control materials.					
31594266	2	69	theme	various	237:243	arg1	agents					259:264	various antimicrobial agents	237:264	various antimicrobial agents	237:264	The incorporation of various antimicrobial agents into resin composites may compromise their physical and mechanical properties hence limiting their applications.					
31594266	2	70	theme	physical	309:316	arg1	properties					333:342	their physical and mechanical properties	303:342	their physical and mechanical properties	303:342	The incorporation of various antimicrobial agents into resin composites may compromise their physical and mechanical properties hence limiting their applications.					
31594266	8	71	theme	1	1267:1267	arg1	%					1268:1268	%	1268:1268	%	1268:1268	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	4	72	theme	contact	798:804	arg1	methods					806:812	agar diffusion test and direct contact methods	767:812	methods	806:812	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	4	73	theme	viscous	731:737	arg1	bacteria					739:746	Actinomyces viscous bacteria	719:746	Actinomyces viscous bacteria	719:746	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	8	74	theme	flowable	1344:1351	arg1	groups					1357:1362	other experimental flowable RBC groups	1325:1362	other experimental flowable RBC groups	1325:1362	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	4	75	theme	RBCs	654:657	arg1	activity					617:624	The antibacterial activity	599:624	The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria	599:746	METHODS The antibacterial activity of microhybrid and flowable RBCs modified with 0, 0.25, 0.5 and 1% w/w chitosan (CS) against Actinomyces viscous bacteria was explored using agar diffusion test and direct contact methods.					
31594266	0	76	theme	dental	80:85	arg1	composites					93:102	chitosan modified dental resin composites	62:102	chitosan modified dental resin composites	62:102	Evaluating antibacterial and surface mechanical properties of chitosan modified dental resin composites.					
31594266	6	77	theme	lawn	1048:1051	arg1	growth					1053:1058	the lawn growth	1044:1058	the lawn growth of Actinomyces viscous	1044:1081	RESULTS The results revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous.					
31594266	5	78	theme	experimental	843:854	arg1	RBCs					856:859	control and experimental RBCs	831:859	control and experimental RBCs	831:859	The hardness of control and experimental RBCs was determined by Vickers hardness (VH) tester.					
31594266	3	79	theme	agent	570:574	arg1	CS					586:587	CS	586:587	CS	586:587	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	3	79	theme	agent	570:574	arg1	chitosan					576:583	novel antimicrobial agent chitosan	550:583	novel antimicrobial agent chitosan (CS)	550:588	OBJECTIVE The aim of the current study is to evaluate the antibacterial activity and the hardness of microhybrid and flowable resin based composites (RBCs) modified using novel antimicrobial agent chitosan (CS).					
31594266	5	80	theme	Vickers	879:885	arg1	VH					897:898	VH	897:898	VH	897:898	The hardness of control and experimental RBCs was determined by Vickers hardness (VH) tester.					
31594266	5	80	theme	Vickers	879:885	arg1	hardness					887:894	Vickers hardness	879:894	Vickers hardness (VH) tester	879:906	The hardness of control and experimental RBCs was determined by Vickers hardness (VH) tester.					
31594266	9	81	theme	%	1404:1404	arg1	CS					1406:1407	0.50% CS	1400:1407	0.50% CS	1400:1407	The microhybrid RBCs consisting of 0.50% CS exhibited significantly higher VH compared to experimental microhybrid RBC group containing 1% CS.					
31594266	0	82	theme	modified	71:78	arg1	composites					93:102	chitosan modified dental resin composites	62:102	chitosan modified dental resin composites	62:102	Evaluating antibacterial and surface mechanical properties of chitosan modified dental resin composites.					
31594266	8	83	theme	flowable	1242:1249	arg1	RBCs					1251:1254	The flowable RBCs	1238:1254	The flowable RBCs containing 1% CS	1238:1271	The flowable RBCs containing 1% CS had significantly higher VH compared to control and other experimental flowable RBC groups.					
31594266	6	84	theme	inhibition	1025:1034	arg1	zone					1036:1039	growth inhibition zone	1018:1039	growth inhibition zone	1018:1039	RESULTS The results revealed that control and experimental flowable and microhybrid RBCs did not demonstrate growth inhibition zone in the lawn growth of Actinomyces viscous.					
34364620	0	0	theme	enhanced	78:85	arg1	adsorption					91:100	enhanced CO2 adsorption	78:100	enhanced CO2 adsorption	78:100	In situ growth of amino-functionalized ZIF-8 on bacterial cellulose foams for enhanced CO2 adsorption.					
34364620	0	1	from	growth	8:13	arg1	cellulose					58:66	bacterial cellulose	48:66	bacterial cellulose	48:66	In situ growth of amino-functionalized ZIF-8 on bacterial cellulose foams for enhanced CO2 adsorption.					
34364620	1	2	theme	great	147:151	arg1	potential					153:161	great potential	147:161	great potential for carbon capture	147:180	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	4	3	theme	cellulose	560:568	arg1	fibers					570:575	cellulose fibers	560:575	cellulose fibers	560:575	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	4	4	contain	have	669:672	arg1	composites					658:667	the composites	654:667	the composites	654:667	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	4	4	contain	have	669:672	arg2	compatibility					702:714	compatibility	702:714	compatibility	702:714	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	4	4	contain	have	669:672	arg2	affinity					689:696	high interface affinity	674:696	high interface affinity	674:696	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	2	5	theme	bacterial	348:356	arg1	cellulose					358:366	biodegradable bacterial cellulose	334:366	biodegradable bacterial cellulose (BC)	334:371	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	2	5	theme	bacterial	348:356	arg1	sustainable					318:328	sustainable	318:328	sustainable	318:328	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	2	5	theme	bacterial	348:356	arg1	substrate					389:397	the substrate	385:397	the substrate for ZIF growth	385:412	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	2	5	theme	bacterial	348:356	arg1	BC					369:370	BC	369:370	BC	369:370	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	1	6	theme	ZIFs	240:243	arg1	application					225:235	the practical application	211:235	the practical application of ZIFs	211:243	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	5	7	dep	1.63 mmol/g	781:791	arg1	1 bar					801:805	1 bar	801:805	1 bar	801:805	The resulting foams presented a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar).					
34364620	5	7	dep	1.63 mmol/g	781:791	arg1	25 °C					794:798	25 °C	794:798	25 °C	794:798	The resulting foams presented a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar).					
34364620	7	8	theme	new	909:911	arg1	avenue					913:918	a new avenue	907:918	a new avenue to construct ZIF/cellulose composites for gas treatment applications	907:987	This work provides a new avenue to construct ZIF/cellulose composites for gas treatment applications.					
34364620	0	9	theme	CO2	87:89	arg1	adsorption					91:100	enhanced CO2 adsorption	78:100	enhanced CO2 adsorption	78:100	In situ growth of amino-functionalized ZIF-8 on bacterial cellulose foams for enhanced CO2 adsorption.					
34364620	2	10	theme	biodegradable	334:346	arg1	cellulose					358:366	biodegradable bacterial cellulose	334:366	biodegradable bacterial cellulose (BC)	334:371	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	2	10	theme	biodegradable	334:346	arg1	sustainable					318:328	sustainable	318:328	sustainable	318:328	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	2	10	theme	biodegradable	334:346	arg1	substrate					389:397	the substrate	385:397	the substrate for ZIF growth	385:412	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	2	10	theme	biodegradable	334:346	arg1	BC					369:370	BC	369:370	BC	369:370	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	3	11	theme	growth	502:507	arg1	approach					509:516	an in situ growth approach	491:516	an in situ growth approach	491:516	Amino-functionalized ZIF-8 (ZIF-8-NH2) was prepared within BC substrate via an in situ growth approach.					
34364620	3	12	theme	Amino-functionalized	415:434	arg1	ZIF-8-NH2					443:451	ZIF-8-NH2	443:451	ZIF-8-NH2	443:451	Amino-functionalized ZIF-8 (ZIF-8-NH2) was prepared within BC substrate via an in situ growth approach.					
34364620	3	12	theme	Amino-functionalized	415:434	arg1	ZIF-8					436:440	Amino-functionalized ZIF-8	415:440	Amino-functionalized ZIF-8 (ZIF-8-NH2)	415:452	Amino-functionalized ZIF-8 (ZIF-8-NH2) was prepared within BC substrate via an in situ growth approach.					
34364620	5	13	theme	resulting	721:729	arg1	foams					731:735	The resulting foams	717:735	The resulting foams	717:735	The resulting foams presented a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar).					
34364620	5	14	theme	CO2	754:756	arg1	capacity					769:776	a high CO2 adsorption capacity	747:776	a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar)	747:806	The resulting foams presented a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar).					
34364620	3	15	theme	BC	474:475	arg1	substrate					477:485	BC substrate	474:485	BC substrate	474:485	Amino-functionalized ZIF-8 (ZIF-8-NH2) was prepared within BC substrate via an in situ growth approach.					
34364620	1	16	theme	carbon	167:172	arg1	capture					174:180	carbon capture	167:180	carbon capture	167:180	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	5	17	theme	1.63 mmol/g	781:791	arg1	capacity					769:776	a high CO2 adsorption capacity	747:776	a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar)	747:806	The resulting foams presented a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar).					
34364620	5	18	theme	high	749:752	arg1	capacity					769:776	a high CO2 adsorption capacity	747:776	a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar)	747:806	The resulting foams presented a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar).					
34364620	0	19	theme	In	0:1	arg1	growth					8:13	In situ growth	0:13	In situ growth of amino-functionalized ZIF-8 on bacterial cellulose	0:66	In situ growth of amino-functionalized ZIF-8 on bacterial cellulose foams for enhanced CO2 adsorption.					
34364620	4	20	theme	hydroxyl	632:639	arg1	groups					641:646	hydroxyl groups	632:646	hydroxyl groups	632:646	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	3	21	dep	in	494:495	arg1	situ					497:500	situ	497:500	situ	497:500	Amino-functionalized ZIF-8 (ZIF-8-NH2) was prepared within BC substrate via an in situ growth approach.					
34364620	2	22	theme	ZIF	403:405	arg1	growth					407:412	ZIF growth	403:412	ZIF growth	403:412	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	1	23	theme	convenient	267:276	arg1	materials					301:309	convenient three-dimensional bulk materials	267:309	convenient three-dimensional bulk materials	267:309	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	6	24	theme	BC	829:830	arg1	foams					832:836	ZIF-8-NH2@BC foams	819:836	ZIF-8-NH2@BC foams	819:836	Moreover, ZIF-8-NH2@BC foams are facile to be regenerated by heating at 80 °C.					
34364620	1	25	theme	Zeolitic	103:110	arg1	ZIFs					136:139	ZIFs	136:139	ZIFs	136:139	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	1	25	theme	Zeolitic	103:110	arg1	frameworks					124:133	Zeolitic imidazolate frameworks	103:133	Zeolitic imidazolate frameworks (ZIFs)	103:140	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	4	26	theme	metal	610:614	arg1	ions					623:626	metal (zinc) ions	610:626	metal (zinc) ions	610:626	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	0	27	theme	ZIF-8	39:43	arg1	growth					8:13	In situ growth	0:13	In situ growth of amino-functionalized ZIF-8 on bacterial cellulose	0:66	In situ growth of amino-functionalized ZIF-8 on bacterial cellulose foams for enhanced CO2 adsorption.					
34364620	1	28	theme	three-dimensional	278:294	arg1	materials					301:309	convenient three-dimensional bulk materials	267:309	convenient three-dimensional bulk materials	267:309	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	6	29	theme	@	828:828	arg1	foams					832:836	ZIF-8-NH2@BC foams	819:836	ZIF-8-NH2@BC foams	819:836	Moreover, ZIF-8-NH2@BC foams are facile to be regenerated by heating at 80 °C.					
34364620	1	30	theme	major	191:195	arg1	development					252:262	the development	248:262	the development of convenient three-dimensional bulk materials	248:309	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	1	30	theme	major	191:195	arg1	challenge					197:205	a major challenge	189:205	a major challenge for the practical application of ZIFs	189:243	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	0	31	theme	amino-functionalized	18:37	arg1	ZIF-8					39:43	amino-functionalized ZIF-8	18:43	amino-functionalized ZIF-8	18:43	In situ growth of amino-functionalized ZIF-8 on bacterial cellulose foams for enhanced CO2 adsorption.					
34364620	1	32	theme	bulk	296:299	arg1	materials					301:309	convenient three-dimensional bulk materials	267:309	convenient three-dimensional bulk materials	267:309	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	5	33	theme	adsorption	758:767	arg1	capacity					769:776	a high CO2 adsorption capacity	747:776	a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar)	747:806	The resulting foams presented a high CO2 adsorption capacity of 1.63 mmol/g (25 °C, 1 bar).					
34364620	6	34	theme	ZIF-8-NH2	819:827	arg1	foams					832:836	ZIF-8-NH2@BC foams	819:836	ZIF-8-NH2@BC foams	819:836	Moreover, ZIF-8-NH2@BC foams are facile to be regenerated by heating at 80 °C.					
34364620	7	35	theme	gas	962:964	arg1	applications					976:987	gas treatment applications	962:987	gas treatment applications	962:987	This work provides a new avenue to construct ZIF/cellulose composites for gas treatment applications.					
34364620	4	36	theme	zinc	617:620	arg1	ions					623:626	metal (zinc) ions	610:626	metal (zinc) ions	610:626	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	0	37	theme	bacterial	48:56	arg1	cellulose					58:66	bacterial cellulose	48:66	bacterial cellulose	48:66	In situ growth of amino-functionalized ZIF-8 on bacterial cellulose foams for enhanced CO2 adsorption.					
34364620	1	38	theme	materials	301:309	arg1	development					252:262	the development	248:262	the development of convenient three-dimensional bulk materials	248:309	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	1	38	theme	materials	301:309	arg1	challenge					197:205	a major challenge	189:205	a major challenge for the practical application of ZIFs	189:243	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	7	39	theme	treatment	966:974	arg1	applications					976:987	gas treatment applications	962:987	gas treatment applications	962:987	This work provides a new avenue to construct ZIF/cellulose composites for gas treatment applications.					
34364620	1	40	theme	imidazolate	112:122	arg1	ZIFs					136:139	ZIFs	136:139	ZIFs	136:139	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	1	40	theme	imidazolate	112:122	arg1	frameworks					124:133	Zeolitic imidazolate frameworks	103:133	Zeolitic imidazolate frameworks (ZIFs)	103:140	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	4	41	theme	ZIF	519:521	arg1	crystals					523:530	ZIF crystals	519:530	ZIF crystals	519:530	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	4	42	theme	high	674:677	arg1	affinity					689:696	high interface affinity	674:696	high interface affinity	674:696	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	4	43	theme	interface	679:687	arg1	affinity					689:696	high interface affinity	674:696	high interface affinity	674:696	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	4	44	theme	chelating	585:593	arg1	effect					595:600	the chelating effect	581:600	the chelating effect between metal (zinc) ions and hydroxyl groups	581:646	ZIF crystals were wrapped uniformly over cellulose fibers and the chelating effect between metal (zinc) ions and hydroxyl groups makes the composites have high interface affinity and compatibility.					
34364620	7	45	theme	ZIF/cellulose	933:945	arg1	composites					947:956	ZIF/cellulose composites	933:956	ZIF/cellulose composites for gas treatment applications	933:987	This work provides a new avenue to construct ZIF/cellulose composites for gas treatment applications.					
34364620	3	46	theme	in	494:495	arg1	approach					509:516	an in situ growth approach	491:516	an in situ growth approach	491:516	Amino-functionalized ZIF-8 (ZIF-8-NH2) was prepared within BC substrate via an in situ growth approach.					
34364620	1	47	theme	practical	215:223	arg1	application					225:235	the practical application	211:235	the practical application of ZIFs	211:243	Zeolitic imidazolate frameworks (ZIFs) hold great potential for carbon capture, while a major challenge for the practical application of ZIFs is the development of convenient three-dimensional bulk materials.					
34364620	0	48	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ growth of amino-functionalized ZIF-8 on bacterial cellulose foams for enhanced CO2 adsorption.					
34364620	2	49	used	used	377:380	arg2	BC					369:370	BC	369:370	BC	369:370	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	2	49	used	used	377:380	arg2	cellulose					358:366	biodegradable bacterial cellulose	334:366	biodegradable bacterial cellulose (BC)	334:371	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	2	49	used	used	377:380	arg2	substrate					389:397	the substrate	385:397	the substrate for ZIF growth	385:412	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
34364620	2	49	used	used	377:380	arg2	sustainable					318:328	sustainable	318:328	sustainable	318:328	Here, sustainable and biodegradable bacterial cellulose (BC) was used as the substrate for ZIF growth.					
31945436	2	0	theme	streptozotocin	598:611	arg1	injection					613:621	streptozotocin injection	598:621	streptozotocin injection	598:621	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	6	1	theme	chemical	1652:1659	arg1	structures					1661:1670	their different chemical structures	1636:1670	their different chemical structures	1636:1670	This study concluded that the antidiabetic effects of ANP, FVP and USP were distinctly different, which might be attributed to their different chemical structures.					
31945436	3	2	theme	alanine	935:941	arg1	ALT					961:963	ALT	961:963	ALT	961:963	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	2	theme	alanine	935:941	arg1	AST					998:1000	AST	998:1000	AST	998:1000	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	2	theme	alanine	935:941	arg1	aminotransferase					943:958	alanine aminotransferase	935:958	alanine aminotransferase (ALT)	935:964	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	3	theme	glucose	710:716	arg1	analysis					657:664	The analysis	653:664	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.	653:1227	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	1	4	theme	rheological	438:448	arg1	potential					288:296	the zeta potential	279:296	the zeta potential	279:296	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	4	theme	rheological	438:448	arg1	properties					450:459	rheological properties	438:459	rheological properties	438:459	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	3	5	theme	FVP	1147:1149	arg1	analysis					657:664	The analysis	653:664	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.	653:1227	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	6	theme	fasting	696:702	arg1	glucose					710:716	fasting blood glucose	696:716	fasting blood glucose	696:716	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	5	7	theme	kidney	1475:1480	arg1	damage					1482:1487	liver and kidney damage	1465:1487	liver and kidney damage caused by diabetes	1465:1506	Histomorphological observation further demonstrated that APs, especially FVP, could attenuate liver and kidney damage caused by diabetes.					
31945436	3	8	theme	oral	728:731	arg1	tolerance					741:749	oral glucose tolerance	728:749	oral glucose tolerance	728:749	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	2	9	theme	weight/day	555:564	arg1	dose					532:535	the dose	528:535	the dose of 400 mg/kg·body weight/day	528:564	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	4	10	theme	FVP	1245:1247	arg1	Intervention					1229:1240	Intervention	1229:1240	Intervention of FVP	1229:1247	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	3	11	dep	indicators	764:773	arg1	including					776:784	including	776:784	including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)	776:897	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	12	theme	antidiabetic	1118:1129	arg1	activities					1131:1140	obvious antidiabetic activities	1110:1140	obvious antidiabetic activities	1110:1140	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	13	theme	better	1158:1163	arg1	effects					1165:1171	better effects	1158:1171	better effects	1158:1171	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	14	theme	blood	752:756	arg1	indicators					764:773	blood lipid indicators	752:773	blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA))	752:898	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	5	15	theme	liver	1465:1469	arg1	damage					1482:1487	liver and kidney damage	1465:1487	liver and kidney damage caused by diabetes	1465:1506	Histomorphological observation further demonstrated that APs, especially FVP, could attenuate liver and kidney damage caused by diabetes.					
31945436	3	16	theme	tolerance	741:749	arg1	analysis					657:664	The analysis	653:664	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.	653:1227	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	1	17	theme	molecular	388:396	arg1	potential					288:296	the zeta potential	279:296	the zeta potential	279:296	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	17	theme	molecular	388:396	arg1	distribution					405:416	molecular weight distribution	388:416	molecular weight distribution	388:416	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	2	18	theme	high	569:572	arg1	sugar					574:578	high sugar and fat diets	569:592	sugar	574:578	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	3	19	theme	insulin	719:725	arg1	analysis					657:664	The analysis	653:664	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.	653:1227	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	20	theme	indicators	764:773	arg1	analysis					657:664	The analysis	653:664	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.	653:1227	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	0	21	theme	polysaccharides	69:83	arg1	properties					30:39	physicochemical properties	14:39	physicochemical properties	14:39	Comparison of physicochemical properties and antidiabetic effects of polysaccharides extracted from three seaweed species.					
31945436	0	21	theme	polysaccharides	69:83	arg1	effects					58:64	antidiabetic effects	45:64	antidiabetic effects	45:64	Comparison of physicochemical properties and antidiabetic effects of polysaccharides extracted from three seaweed species.					
31945436	3	22	theme	low	829:831	arg1	LDL-C					866:870	LDL-C	866:870	LDL-C	866:870	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	22	theme	low	829:831	arg1	cholesterol					853:863	low density lipoprotein cholesterol	829:863	low density lipoprotein cholesterol (LDL-C)	829:871	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	1	23	theme	holding	313:319	arg1	capacity					321:328	oil holding capacity	309:328	oil holding capacity	309:328	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	3	24	contain	possessed	1100:1108	arg2	activities					1131:1140	obvious antidiabetic activities	1110:1140	obvious antidiabetic activities	1110:1140	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	24	contain	possessed	1100:1108	arg1	APs					1096:1098	APs	1096:1098	APs	1096:1098	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	4	25	theme	bile	1267:1270	arg1	level					1283:1287	the total bile acid (TBA) level	1257:1287	the total bile acid (TBA) level	1257:1287	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	0	26	theme	seaweed	106:112	arg1	species					114:120	three seaweed species	100:120	three seaweed species	100:120	Comparison of physicochemical properties and antidiabetic effects of polysaccharides extracted from three seaweed species.					
31945436	3	27	theme	aspartate	970:978	arg1	aminotransferase					980:995	aspartate aminotransferase	970:995	aspartate aminotransferase	970:995	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	7	28	dep	relationship	1707:1718	arg1	the					1684:1686	the	1684:1686	the	1684:1686	Therefore, the structure-activity relationship and antidiabetic mechanism of APs will be our future research direction.					
31945436	2	29	theme	diabetic	638:645	arg1	rats					647:650	type 2 diabetic rats	631:650	type 2 diabetic rats	631:650	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	4	30	theme	lipoprotein	1315:1325	arg1	HDL-C					1340:1344	HDL-C	1340:1344	HDL-C	1340:1344	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	4	30	theme	lipoprotein	1315:1325	arg1	cholesterol					1327:1337	elevated high density lipoprotein cholesterol	1293:1337	elevated high density lipoprotein cholesterol (HDL-C)	1293:1345	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	7	31	theme	future	1766:1771	arg1	direction					1782:1790	our future research direction	1762:1790	our future research direction	1762:1790	Therefore, the structure-activity relationship and antidiabetic mechanism of APs will be our future research direction.					
31945436	3	32	theme	total	786:790	arg1	FFA					894:896	FFA	894:896	FFA	894:896	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	32	theme	total	786:790	arg1	TG					824:825	TG	824:825	TG	824:825	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	32	theme	total	786:790	arg1	TC					805:806	TC	805:806	TC	805:806	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	32	theme	total	786:790	arg1	triglyceride					810:821	triglyceride	810:821	triglyceride	810:821	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	32	theme	total	786:790	arg1	cholesterol					792:802	total cholesterol	786:802	total cholesterol (TC)	786:807	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	33	theme	lipoprotein	841:851	arg1	LDL-C					866:870	LDL-C	866:870	LDL-C	866:870	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	33	theme	lipoprotein	841:851	arg1	cholesterol					853:863	low density lipoprotein cholesterol	829:863	low density lipoprotein cholesterol (LDL-C)	829:871	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	6	34	theme	antidiabetic	1539:1550	arg1	different					1596:1604	different	1596:1604	different	1596:1604	This study concluded that the antidiabetic effects of ANP, FVP and USP were distinctly different, which might be attributed to their different chemical structures.					
31945436	6	34	theme	antidiabetic	1539:1550	arg1	effects					1552:1558	the antidiabetic effects	1535:1558	the antidiabetic effects of ANP, FVP and USP	1535:1578	This study concluded that the antidiabetic effects of ANP, FVP and USP were distinctly different, which might be attributed to their different chemical structures.					
31945436	7	35	theme	antidiabetic	1724:1735	arg1	mechanism					1737:1745	antidiabetic mechanism	1724:1745	antidiabetic mechanism	1724:1745	Therefore, the structure-activity relationship and antidiabetic mechanism of APs will be our future research direction.					
31945436	3	36	theme	free	877:880	arg1	acid					888:891	free fatty acid	877:891	free fatty acid	877:891	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	0	37	theme	physicochemical	14:28	arg1	properties					30:39	physicochemical properties	14:39	physicochemical properties	14:39	Comparison of physicochemical properties and antidiabetic effects of polysaccharides extracted from three seaweed species.					
31945436	7	38	theme	APs	1750:1752	arg1	relationship					1707:1718	structure-activity relationship	1688:1718	structure-activity relationship	1688:1718	Therefore, the structure-activity relationship and antidiabetic mechanism of APs will be our future research direction.					
31945436	7	38	theme	APs	1750:1752	arg1	mechanism					1737:1745	antidiabetic mechanism	1724:1745	antidiabetic mechanism	1724:1745	Therefore, the structure-activity relationship and antidiabetic mechanism of APs will be our future research direction.					
31945436	4	39	theme	high	1302:1305	arg1	lipoprotein					1315:1325	elevated high density lipoprotein	1293:1325	elevated high density lipoprotein cholesterol (HDL-C)	1293:1345	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	1	40	theme	zeta	283:286	arg1	composition					375:385	organic element composition	359:385	organic element composition	359:385	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	40	theme	zeta	283:286	arg1	potential					288:296	the zeta potential	279:296	the zeta potential	279:296	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	40	theme	zeta	283:286	arg1	microstructure					419:432	microstructure	419:432	microstructure	419:432	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	40	theme	zeta	283:286	arg1	properties					450:459	rheological properties	438:459	rheological properties	438:459	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	40	theme	zeta	283:286	arg1	composition					346:356	monosaccharide composition	331:356	monosaccharide composition	331:356	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	40	theme	zeta	283:286	arg1	distribution					405:416	molecular weight distribution	388:416	molecular weight distribution	388:416	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	3	41	theme	function	1014:1021	arg1	profiles					1023:1030	renal function profiles	1008:1030	renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN))	1008:1082	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	2	42	theme	Antidiabetic	462:473	arg1	effects					475:481	Antidiabetic effects	462:481	Antidiabetic effects of APs	462:488	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	4	43	theme	rats	1365:1368	arg1	level					1283:1287	the total bile acid (TBA) level	1257:1287	the total bile acid (TBA) level	1257:1287	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	4	43	theme	rats	1365:1368	arg1	cholesterol					1327:1337	elevated high density lipoprotein cholesterol	1293:1337	elevated high density lipoprotein cholesterol (HDL-C)	1293:1345	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	4	43	theme	rats	1365:1368	arg1	HDL-C					1340:1344	HDL-C	1340:1344	HDL-C	1340:1344	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	0	44	theme	effects	58:64	arg1	Comparison					0:9	Comparison	0:9	Comparison of physicochemical properties and antidiabetic effects of polysaccharides	0:83	Comparison of physicochemical properties and antidiabetic effects of polysaccharides extracted from three seaweed species.					
31945436	3	45	theme	weight	674:679	arg1	analysis					657:664	The analysis	653:664	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.	653:1227	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	46	theme	uric	1044:1047	arg1	BUN					1078:1080	BUN	1078:1080	BUN	1078:1080	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	46	theme	uric	1044:1047	arg1	UA					1055:1056	UA	1055:1056	UA	1055:1056	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	46	theme	uric	1044:1047	arg1	acid					1049:1052	uric acid	1044:1052	uric acid (UA)	1044:1057	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	47	theme	liver	901:905	arg1	indexes					916:922	liver function indexes	901:922	liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST))	901:1002	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	2	48	from	dose	532:535	arg1	intervention					512:523	oral intervention	507:523	oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets	507:592	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	3	49	theme	urea	1063:1066	arg1	nitrogen					1068:1075	urea nitrogen	1063:1075	urea nitrogen	1063:1075	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	6	50	theme	different	1642:1650	arg1	structures					1661:1670	their different chemical structures	1636:1670	their different chemical structures	1636:1670	This study concluded that the antidiabetic effects of ANP, FVP and USP were distinctly different, which might be attributed to their different chemical structures.					
31945436	3	51	theme	water	682:686	arg1	intake					688:693	water intake	682:693	water intake	682:693	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	2	52	theme	fat	584:586	arg1	diets					588:592	high sugar and fat diets	569:592	diets	588:592	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	3	53	theme	blood	704:708	arg1	glucose					710:716	fasting blood glucose	696:716	fasting blood glucose	696:716	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	54	theme	glucose	733:739	arg1	tolerance					741:749	oral glucose tolerance	728:749	oral glucose tolerance	728:749	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	2	55	theme	400 mg/kg·body	540:553	arg1	weight/day					555:564	400 mg/kg·body weight/day	540:564	400 mg/kg·body weight/day	540:564	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	1	56	theme	element	367:373	arg1	composition					375:385	organic element composition	359:385	organic element composition	359:385	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	56	theme	element	367:373	arg1	potential					288:296	the zeta potential	279:296	the zeta potential	279:296	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	3	57	theme	lipid	758:762	arg1	indicators					764:773	blood lipid indicators	752:773	blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA))	752:898	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	1	58	theme	algae	129:133	arg1	APs					152:154	APs	152:154	APs	152:154	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	58	theme	algae	129:133	arg1	polysaccharides					135:149	Three algae polysaccharides	123:149	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP)	123:251	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	3	59	theme	obvious	1110:1116	arg1	activities					1131:1140	obvious antidiabetic activities	1110:1140	obvious antidiabetic activities	1110:1140	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	2	60	from	intervention	512:523	arg1	sugar					574:578	high sugar and fat diets	569:592	sugar	574:578	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	2	60	from	intervention	512:523	arg1	diets					588:592	high sugar and fat diets	569:592	diets	588:592	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	4	61	dep	level	1283:1287	arg1	level					1347:1351	level	1347:1351	level	1347:1351	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	3	62	theme	FFA	1202:1204	arg1	levels					1192:1197	the levels	1188:1197	the levels of FFA, AST, ALT, UA and BUN	1188:1226	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	1	63	theme	weight	398:403	arg1	potential					288:296	the zeta potential	279:296	the zeta potential	279:296	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	63	theme	weight	398:403	arg1	distribution					405:416	molecular weight distribution	388:416	molecular weight distribution	388:416	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	4	64	theme	acid	1272:1275	arg1	level					1283:1287	the total bile acid (TBA) level	1257:1287	the total bile acid (TBA) level	1257:1287	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	6	65	theme	USP	1576:1578	arg1	different					1596:1604	different	1596:1604	different	1596:1604	This study concluded that the antidiabetic effects of ANP, FVP and USP were distinctly different, which might be attributed to their different chemical structures.					
31945436	6	65	theme	USP	1576:1578	arg1	effects					1552:1558	the antidiabetic effects	1535:1558	the antidiabetic effects of ANP, FVP and USP	1535:1578	This study concluded that the antidiabetic effects of ANP, FVP and USP were distinctly different, which might be attributed to their different chemical structures.					
31945436	3	66	theme	ALT	1212:1214	arg1	levels					1192:1197	the levels	1188:1197	the levels of FFA, AST, ALT, UA and BUN	1188:1226	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	4	67	theme	TBA	1278:1280	arg1	level					1283:1287	the total bile acid (TBA) level	1257:1287	the total bile acid (TBA) level	1257:1287	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	3	68	dep	profiles	1023:1030	arg1	comprising					1033:1042	comprising	1033:1042	comprising uric acid (UA) and urea nitrogen (BUN)	1033:1081	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	1	69	theme	oil	309:311	arg1	capacity					321:328	oil holding capacity	309:328	oil holding capacity	309:328	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	7	70	theme	structure-activity	1688:1705	arg1	relationship					1707:1718	structure-activity relationship	1688:1718	structure-activity relationship	1688:1718	Therefore, the structure-activity relationship and antidiabetic mechanism of APs will be our future research direction.					
31945436	3	71	theme	density	833:839	arg1	LDL-C					866:870	LDL-C	866:870	LDL-C	866:870	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	71	theme	density	833:839	arg1	cholesterol					853:863	low density lipoprotein cholesterol	829:863	low density lipoprotein cholesterol (LDL-C)	829:871	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	2	72	theme	APs	486:488	arg1	effects					475:481	Antidiabetic effects	462:481	Antidiabetic effects of APs	462:488	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	6	73	theme	FVP	1568:1570	arg1	different					1596:1604	different	1596:1604	different	1596:1604	This study concluded that the antidiabetic effects of ANP, FVP and USP were distinctly different, which might be attributed to their different chemical structures.					
31945436	6	73	theme	FVP	1568:1570	arg1	effects					1552:1558	the antidiabetic effects	1535:1558	the antidiabetic effects of ANP, FVP and USP	1535:1578	This study concluded that the antidiabetic effects of ANP, FVP and USP were distinctly different, which might be attributed to their different chemical structures.					
31945436	3	74	theme	AST	1207:1209	arg1	levels					1192:1197	the levels	1188:1197	the levels of FFA, AST, ALT, UA and BUN	1188:1226	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	1	75	theme	organic	359:365	arg1	composition					375:385	organic element composition	359:385	organic element composition	359:385	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	75	theme	organic	359:365	arg1	potential					288:296	the zeta potential	279:296	the zeta potential	279:296	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	7	76	theme	research	1773:1780	arg1	direction					1782:1790	our future research direction	1762:1790	our future research direction	1762:1790	Therefore, the structure-activity relationship and antidiabetic mechanism of APs will be our future research direction.					
31945436	6	77	theme	ANP	1563:1565	arg1	different					1596:1604	different	1596:1604	different	1596:1604	This study concluded that the antidiabetic effects of ANP, FVP and USP were distinctly different, which might be attributed to their different chemical structures.					
31945436	6	77	theme	ANP	1563:1565	arg1	effects					1552:1558	the antidiabetic effects	1535:1558	the antidiabetic effects of ANP, FVP and USP	1535:1578	This study concluded that the antidiabetic effects of ANP, FVP and USP were distinctly different, which might be attributed to their different chemical structures.					
31945436	3	78	theme	BUN	1224:1226	arg1	levels					1192:1197	the levels	1188:1197	the levels of FFA, AST, ALT, UA and BUN	1188:1226	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	4	79	theme	total	1261:1265	arg1	level					1283:1287	the total bile acid (TBA) level	1257:1287	the total bile acid (TBA) level	1257:1287	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	1	80	theme	monosaccharide	331:344	arg1	potential					288:296	the zeta potential	279:296	the zeta potential	279:296	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	1	80	theme	monosaccharide	331:344	arg1	composition					346:356	monosaccharide composition	331:356	monosaccharide composition	331:356	Three algae polysaccharides (APs) extracted from Ascophyllum nodosum (ANP), Fucus vesiculosus (FVP) and Undaria Pinnatifida (USP) significantly differed in the zeta potential, water and oil holding capacity, monosaccharide composition, organic element composition, molecular weight distribution, microstructure and rheological properties.					
31945436	2	81	theme	oral	507:510	arg1	intervention					512:523	oral intervention	507:523	oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets	507:592	Antidiabetic effects of APs were compared by oral intervention at the dose of 400 mg/kg·body weight/day in high sugar and fat diets and streptozotocin injection induced type 2 diabetic rats.					
31945436	3	82	theme	UA	1217:1218	arg1	levels					1192:1197	the levels	1188:1197	the levels of FFA, AST, ALT, UA and BUN	1188:1226	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	4	83	theme	density	1307:1313	arg1	lipoprotein					1315:1325	elevated high density lipoprotein	1293:1325	elevated high density lipoprotein cholesterol (HDL-C)	1293:1345	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	0	84	theme	properties	30:39	arg1	Comparison					0:9	Comparison	0:9	Comparison of physicochemical properties and antidiabetic effects of polysaccharides	0:83	Comparison of physicochemical properties and antidiabetic effects of polysaccharides extracted from three seaweed species.					
31945436	3	85	theme	fatty	882:886	arg1	acid					888:891	free fatty acid	877:891	free fatty acid	877:891	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	0	86	theme	antidiabetic	45:56	arg1	effects					58:64	antidiabetic effects	45:64	antidiabetic effects	45:64	Comparison of physicochemical properties and antidiabetic effects of polysaccharides extracted from three seaweed species.					
31945436	3	87	theme	renal	1008:1012	arg1	profiles					1023:1030	renal function profiles	1008:1030	renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN))	1008:1082	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	5	88	theme	Histomorphological	1371:1388	arg1	observation					1390:1400	Histomorphological observation	1371:1400	Histomorphological observation	1371:1400	Histomorphological observation further demonstrated that APs, especially FVP, could attenuate liver and kidney damage caused by diabetes.					
31945436	4	89	theme	elevated	1293:1300	arg1	lipoprotein					1315:1325	elevated high density lipoprotein	1293:1325	elevated high density lipoprotein cholesterol (HDL-C)	1293:1345	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	3	90	theme	body	669:672	arg1	weight					674:679	body weight	669:679	body weight	669:679	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	4	91	theme	diabetic	1356:1363	arg1	rats					1365:1368	diabetic rats	1356:1368	diabetic rats	1356:1368	Intervention of FVP reduced the total bile acid (TBA) level and elevated high density lipoprotein cholesterol (HDL-C) level of diabetic rats.					
31945436	3	92	dep	indexes	916:922	arg1	involving					925:933	involving	925:933	involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)	925:1001	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	93	theme	function	907:914	arg1	indexes					916:922	liver function indexes	901:922	liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST))	901:1002	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
31945436	3	94	theme	intake	688:693	arg1	analysis					657:664	The analysis	653:664	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.	653:1227	The analysis of body weight, water intake, fasting blood glucose, insulin, oral glucose tolerance, blood lipid indicators (including total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA)), liver function indexes (involving alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and renal function profiles (comprising uric acid (UA) and urea nitrogen (BUN)) showed that APs possessed obvious antidiabetic activities, and FVP showed better effects in controlling the levels of FFA, AST, ALT, UA and BUN.					
32439612	14	0	theme	matrix	2016:2021	arg1	removal					1997:2003	The removal	1993:2003	The removal of protein matrix	1993:2021	The removal of protein matrix induce a reduction of Young's modulus and maximum stress, leaving the chitin matrix structurally unaltered.					
32439612	8	1	theme	overall	1060:1066	arg1	folding					1076:1082	the overall correct folding	1056:1082	the overall correct folding of the proteins	1056:1098	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	7	2	theme	chitin	841:846	arg1	matrix					848:853	the chitin matrix	837:853	the chitin matrix	837:853	The data analysis showed that chemical treatments did not modify the structure of the chitin matrix.					
32439612	4	3	theme	research	369:376	arg1	theme					378:382	this research theme	364:382	this research theme	364:382	Here, this research theme is studied using the pen of Loligo vulgaris as a model material of chitin-protein composites.					
32439612	8	4	theme	proteins	1091:1098	arg1	folding					1076:1082	the overall correct folding	1056:1082	the overall correct folding of the proteins	1056:1098	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	12	5	dep	STATEMENT	1671:1679	arg1	addressed					1720:1728	addressed	1720:1728	has addressed on how proteins influence chitin-composite material's mechanical properties	1716:1804	STATEMENT OF SIGNIFICANCE: To date, no study has addressed on how proteins influence chitin-composite material's mechanical properties.					
32439612	8	6	theme	proteins	1128:1135	arg1	removal					1117:1123	the whole removal	1107:1123	(iii) the whole removal of proteins	1101:1135	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	11	7	theme	polymeric	1609:1617	arg1	composites					1619:1628	other biogenic polymeric composites	1594:1628	other biogenic polymeric composites	1594:1628	In conclusion, this knowledge can explain the physio-chemical properties of other biogenic polymeric composites and inspire the design of new materials.					
32439612	10	8	dep	links	1406:1410	arg1	avoiding					1431:1438	avoiding	1431:1438	avoiding them to slide one on the other	1431:1469	This reinforcement links the chitin fibrils avoiding them to slide one on the other and maximizing their resistance and stiffness.					
32439612	10	8	dep	links	1406:1410	arg1	maximizing					1475:1484	maximizing	1475:1484	maximizing their resistance and stiffness	1475:1515	This reinforcement links the chitin fibrils avoiding them to slide one on the other and maximizing their resistance and stiffness.					
32439612	1	9	theme	many	89:92	arg1	materials					103:111	many biogenic materials	89:111	many biogenic materials	89:111	In many biogenic materials, chitin chains are assembled in fibrils that are wrapped by a protein fold.					
32439612	11	10	theme	other	1594:1598	arg1	composites					1619:1628	other biogenic polymeric composites	1594:1628	other biogenic polymeric composites	1594:1628	In conclusion, this knowledge can explain the physio-chemical properties of other biogenic polymeric composites and inspire the design of new materials.					
32439612	16	11	theme	chitin	2389:2394	arg1	fibrils					2396:2402	crystalline chitin fibrils	2377:2402	crystalline chitin fibrils avoiding them to slide one	2377:2429	In conclusion, this research shows that the proteins act as a stiff matrix reinforced by di-sulfide bridges that link crystalline chitin fibrils avoiding them to slide one on the other.					
32439612	15	12	theme	elongation	2158:2167	arg1	measure					2135:2141	The measure	2131:2141	The measure of the maximum elongation	2131:2167	The measure of the maximum elongation shows that the chitin fibrils slide on each other only after removing the protein matrix.					
32439612	12	13	theme	chitin-composite	1756:1771	arg1	properties					1795:1804	chitin-composite material's mechanical properties	1756:1804	chitin-composite material's mechanical properties	1756:1804	STATEMENT OF SIGNIFICANCE: To date, no study has addressed on how proteins influence chitin-composite material's mechanical properties.					
32439612	4	14	theme	Loligo	412:417	arg1	vulgaris					419:426	Loligo vulgaris	412:426	Loligo vulgaris	412:426	Here, this research theme is studied using the pen of Loligo vulgaris as a model material of chitin-protein composites.					
32439612	10	15	theme	chitin	1416:1421	arg1	fibrils					1423:1429	the chitin fibrils	1412:1429	the chitin fibrils	1412:1429	This reinforcement links the chitin fibrils avoiding them to slide one on the other and maximizing their resistance and stiffness.					
32439612	13	16	theme	protein	1883:1889	arg1	bonds					1901:1905	protein disulfide bonds	1883:1905	protein disulfide bonds	1883:1905	Here we show that the Young's modulus and the maximum stress mainly rely on protein disulfide bonds, the inter-proteins ones and those controlling the folding of chitin-binding domains.					
32439612	15	17	theme	maximum	2150:2156	arg1	elongation					2158:2167	the maximum elongation	2146:2167	the maximum elongation	2146:2167	The measure of the maximum elongation shows that the chitin fibrils slide on each other only after removing the protein matrix.					
32439612	1	18	theme	chitin	114:119	arg1	chains					121:126	chitin chains	114:126	chitin chains	114:126	In many biogenic materials, chitin chains are assembled in fibrils that are wrapped by a protein fold.					
32439612	5	19	used	used	503:506	arg2	treatments					487:496	Chemical treatments	478:496	Chemical treatments	478:496	Chemical treatments were used to change the interactions involving only the proteic phase, through unfolding and/or degradation processes.					
32439612	6	20	theme	structural	631:640	arg1	parameters					657:666	Successively, structural and mechanical parameters	617:666	Successively, structural and mechanical parameters	617:666	Successively, structural and mechanical parameters were examined using spectroscopy, microscopy, X-ray diffractometry, and tensile tests.					
32439612	15	21	theme	protein	2243:2249	arg1	matrix					2251:2256	the protein matrix	2239:2256	the protein matrix	2239:2256	The measure of the maximum elongation shows that the chitin fibrils slide on each other only after removing the protein matrix.					
32439612	6	22	theme	Successively	617:628	arg1	parameters					657:666	Successively, structural and mechanical parameters	617:666	Successively, structural and mechanical parameters	617:666	Successively, structural and mechanical parameters were examined using spectroscopy, microscopy, X-ray diffractometry, and tensile tests.					
32439612	0	23	theme	natural	52:58	arg1	composite					75:83	a natural chitin-protein composite	50:83	a natural chitin-protein composite	50:83	Influence of proteins on mechanical properties of a natural chitin-protein composite.					
32439612	8	24	theme	σmax	1173:1176	arg1	increase					1194:1201	an increase	1191:1201	an increase in the maximum elongation	1191:1227	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	24	theme	σmax	1173:1176	arg1	decrease					1147:1154	a decrease	1145:1154	a decrease of E (> 90%) and σmax (> 80%)	1145:1184	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	14	25	theme	chitin	2093:2098	arg1	matrix					2100:2105	the chitin matrix	2089:2105	the chitin matrix structurally unaltered	2089:2128	The removal of protein matrix induce a reduction of Young's modulus and maximum stress, leaving the chitin matrix structurally unaltered.					
32439612	0	26	theme	composite	75:83	arg1	properties					36:45	mechanical properties	25:45	mechanical properties of a natural chitin-protein composite	25:83	Influence of proteins on mechanical properties of a natural chitin-protein composite.					
32439612	4	27	theme	composites	466:475	arg1	material					439:446	a model material	431:446	a model material of chitin-protein composites	431:475	Here, this research theme is studied using the pen of Loligo vulgaris as a model material of chitin-protein composites.					
32439612	4	27	theme	composites	466:475	arg1	pen					405:407	the pen	401:407	the pen of Loligo vulgaris	401:426	Here, this research theme is studied using the pen of Loligo vulgaris as a model material of chitin-protein composites.					
32439612	8	28	theme	>	1179:1179	arg1	%					1183:1183	> 80%	1179:1183	> 80%	1179:1183	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	28	theme	>	1179:1179	arg1	σmax					1173:1176	σmax	1173:1176	σmax (> 80%)	1173:1184	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	5	29	theme	Chemical	478:485	arg1	treatments					487:496	Chemical treatments	478:496	Chemical treatments	478:496	Chemical treatments were used to change the interactions involving only the proteic phase, through unfolding and/or degradation processes.					
32439612	15	30	theme	chitin	2184:2189	arg1	fibrils					2191:2197	the chitin fibrils	2180:2197	the chitin fibrils	2180:2197	The measure of the maximum elongation shows that the chitin fibrils slide on each other only after removing the protein matrix.					
32439612	4	31	theme	model	433:437	arg1	material					439:446	a model material	431:446	a model material of chitin-protein composites	431:475	Here, this research theme is studied using the pen of Loligo vulgaris as a model material of chitin-protein composites.					
32439612	4	31	theme	model	433:437	arg1	pen					405:407	the pen	401:407	the pen of Loligo vulgaris	401:426	Here, this research theme is studied using the pen of Loligo vulgaris as a model material of chitin-protein composites.					
32439612	8	32	theme	bonds	1010:1014	arg1	presence					984:991	the presence	980:991	the presence of the disulfide bonds	980:1014	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	9	33	theme	disulfide	1368:1376	arg1	bridges					1378:1384	disulfide bridges	1368:1384	disulfide bridges	1368:1384	These observations indicate that in the chitin matrix the proteins act as a strengthener, which efficacy is controlled by the presence of disulfide bridges.					
32439612	15	34	from	slide	2199:2203	arg1	other					2213:2217	other	2213:2217	other	2213:2217	The measure of the maximum elongation shows that the chitin fibrils slide on each other only after removing the protein matrix.					
32439612	8	35	theme	maximum	948:954	arg1	σmax					964:967	σmax	964:967	σmax	964:967	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	35	theme	maximum	948:954	arg1	stress					956:961	the maximum stress	944:961	(i) the maximum stress (σmax)	940:968	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	0	36	theme	proteins	13:20	arg1	Influence					0:8	Influence	0:8	Influence of proteins on mechanical properties of a natural chitin-protein composite.	0:84	Influence of proteins on mechanical properties of a natural chitin-protein composite.					
32439612	8	37	theme	mechanical	888:897	arg1	analysis					904:911	the mechanical test analysis	884:911	the mechanical test analysis	884:911	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	6	38	theme	mechanical	646:655	arg1	parameters					657:666	Successively, structural and mechanical parameters	617:666	Successively, structural and mechanical parameters	617:666	Successively, structural and mechanical parameters were examined using spectroscopy, microscopy, X-ray diffractometry, and tensile tests.					
32439612	0	39	theme	mechanical	25:34	arg1	properties					36:45	mechanical properties	25:45	mechanical properties of a natural chitin-protein composite	25:83	Influence of proteins on mechanical properties of a natural chitin-protein composite.					
32439612	12	40	theme	SIGNIFICANCE	1684:1695	arg1	STATEMENT					1671:1679	STATEMENT	1671:1679	STATEMENT OF SIGNIFICANCE: To date, no study has addressed on how proteins influence chitin-composite material's mechanical properties.	1671:1805	STATEMENT OF SIGNIFICANCE: To date, no study has addressed on how proteins influence chitin-composite material's mechanical properties.					
32439612	8	41	theme	maximum	1210:1216	arg1	elongation					1218:1227	the maximum elongation	1206:1227	the maximum elongation	1206:1227	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	11	42	theme	materials	1660:1668	arg1	design					1646:1651	the design	1642:1651	the design of new materials	1642:1668	In conclusion, this knowledge can explain the physio-chemical properties of other biogenic polymeric composites and inspire the design of new materials.					
32439612	8	43	theme	Young	1026:1030	arg1	E					1043:1043	E	1043:1043	E	1043:1043	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	43	theme	Young	1026:1030	arg1	modulus					1034:1040	the Young's modulus	1022:1040	the Young's modulus (E)	1022:1044	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	44	theme	following	917:925	arg1	conclusions					927:937	the following conclusions	913:937	the following conclusions	913:937	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	13	45	theme	domains	1984:1990	arg1	folding					1958:1964	the folding	1954:1964	the folding of chitin-binding domains	1954:1990	Here we show that the Young's modulus and the maximum stress mainly rely on protein disulfide bonds, the inter-proteins ones and those controlling the folding of chitin-binding domains.					
32439612	14	46	theme	protein	2008:2014	arg1	matrix					2016:2021	protein matrix	2008:2021	protein matrix	2008:2021	The removal of protein matrix induce a reduction of Young's modulus and maximum stress, leaving the chitin matrix structurally unaltered.					
32439612	2	47	theme	intra-	254:259	arg1	interactions					272:283	intra- and inter- interactions	254:283	intra- and inter- interactions among chitin and proteins	254:309	In them, the mechanical properties are supposed to be related to intra- and inter- interactions among chitin and proteins.					
32439612	7	48	theme	matrix	848:853	arg1	structure					824:832	the structure	820:832	the structure of the chitin matrix	820:853	The data analysis showed that chemical treatments did not modify the structure of the chitin matrix.					
32439612	8	49	theme	correct	1068:1074	arg1	folding					1076:1082	the overall correct folding	1056:1082	the overall correct folding of the proteins	1056:1098	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	50	theme	whole	1111:1115	arg1	removal					1117:1123	the whole removal	1107:1123	(iii) the whole removal of proteins	1101:1135	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	51	dep	allowed	861:867	arg1	derive					872:877	derive	872:877	allowed to derive from the mechanical test analysis the following conclusions	861:937	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	52	from	increase	1194:1201	arg1	elongation					1218:1227	the maximum elongation	1206:1227	the maximum elongation	1206:1227	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	12	53	theme	material	1773:1780	arg1	properties					1795:1804	chitin-composite material's mechanical properties	1756:1804	chitin-composite material's mechanical properties	1756:1804	STATEMENT OF SIGNIFICANCE: To date, no study has addressed on how proteins influence chitin-composite material's mechanical properties.					
32439612	16	54	theme	crystalline	2377:2387	arg1	fibrils					2396:2402	crystalline chitin fibrils	2377:2402	crystalline chitin fibrils avoiding them to slide one	2377:2429	In conclusion, this research shows that the proteins act as a stiff matrix reinforced by di-sulfide bridges that link crystalline chitin fibrils avoiding them to slide one on the other.					
32439612	11	55	theme	composites	1619:1628	arg1	properties					1580:1589	the physio-chemical properties	1560:1589	the physio-chemical properties of other biogenic polymeric composites	1560:1628	In conclusion, this knowledge can explain the physio-chemical properties of other biogenic polymeric composites and inspire the design of new materials.					
32439612	2	56	theme	inter-	265:270	arg1	interactions					272:283	intra- and inter- interactions	254:283	intra- and inter- interactions among chitin and proteins	254:309	In them, the mechanical properties are supposed to be related to intra- and inter- interactions among chitin and proteins.					
32439612	9	57	theme	chitin	1270:1275	arg1	matrix					1277:1282	the chitin matrix	1266:1282	the chitin matrix	1266:1282	These observations indicate that in the chitin matrix the proteins act as a strengthener, which efficacy is controlled by the presence of disulfide bridges.					
32439612	12	58	theme	mechanical	1784:1793	arg1	properties					1795:1804	chitin-composite material's mechanical properties	1756:1804	chitin-composite material's mechanical properties	1756:1804	STATEMENT OF SIGNIFICANCE: To date, no study has addressed on how proteins influence chitin-composite material's mechanical properties.					
32439612	11	59	theme	biogenic	1600:1607	arg1	composites					1619:1628	other biogenic polymeric composites	1594:1628	other biogenic polymeric composites	1594:1628	In conclusion, this knowledge can explain the physio-chemical properties of other biogenic polymeric composites and inspire the design of new materials.					
32439612	13	60	theme	inter-proteins	1912:1925	arg1	ones					1927:1930	the inter-proteins ones	1908:1930	the inter-proteins ones	1908:1930	Here we show that the Young's modulus and the maximum stress mainly rely on protein disulfide bonds, the inter-proteins ones and those controlling the folding of chitin-binding domains.					
32439612	2	61	theme	mechanical	202:211	arg1	related					243:249	related	243:249	related	243:249	In them, the mechanical properties are supposed to be related to intra- and inter- interactions among chitin and proteins.					
32439612	2	61	theme	mechanical	202:211	arg1	properties					213:222	the mechanical properties	198:222	the mechanical properties	198:222	In them, the mechanical properties are supposed to be related to intra- and inter- interactions among chitin and proteins.					
32439612	1	62	theme	biogenic	94:101	arg1	materials					103:111	many biogenic materials	89:111	many biogenic materials	89:111	In many biogenic materials, chitin chains are assembled in fibrils that are wrapped by a protein fold.					
32439612	4	63	theme	vulgaris	419:426	arg1	material					439:446	a model material	431:446	a model material of chitin-protein composites	431:475	Here, this research theme is studied using the pen of Loligo vulgaris as a model material of chitin-protein composites.					
32439612	4	63	theme	vulgaris	419:426	arg1	pen					405:407	the pen	401:407	the pen of Loligo vulgaris	401:426	Here, this research theme is studied using the pen of Loligo vulgaris as a model material of chitin-protein composites.					
32439612	0	64	from	Influence	0:8	arg1	properties					36:45	mechanical properties	25:45	mechanical properties of a natural chitin-protein composite	25:83	Influence of proteins on mechanical properties of a natural chitin-protein composite.					
32439612	8	65	theme	E	1159:1159	arg1	increase					1194:1201	an increase	1191:1201	an increase in the maximum elongation	1191:1227	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	65	theme	E	1159:1159	arg1	decrease					1147:1154	a decrease	1145:1154	a decrease of E (> 90%) and σmax (> 80%)	1145:1184	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	13	66	theme	disulfide	1891:1899	arg1	bonds					1901:1905	protein disulfide bonds	1883:1905	protein disulfide bonds	1883:1905	Here we show that the Young's modulus and the maximum stress mainly rely on protein disulfide bonds, the inter-proteins ones and those controlling the folding of chitin-binding domains.					
32439612	16	67	theme	stiff	2321:2325	arg1	matrix					2327:2332	a stiff matrix	2319:2332	a stiff matrix reinforced by di-sulfide bridges that link crystalline chitin fibrils avoiding them to slide one on the other	2319:2442	In conclusion, this research shows that the proteins act as a stiff matrix reinforced by di-sulfide bridges that link crystalline chitin fibrils avoiding them to slide one on the other.					
32439612	16	67	theme	stiff	2321:2325	arg1	proteins					2303:2310	the proteins	2299:2310	the proteins	2299:2310	In conclusion, this research shows that the proteins act as a stiff matrix reinforced by di-sulfide bridges that link crystalline chitin fibrils avoiding them to slide one on the other.					
32439612	8	68	theme	>	1162:1162	arg1	E					1159:1159	E	1159:1159	E (> 90%)	1159:1167	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	68	theme	>	1162:1162	arg1	%					1166:1166	> 90%	1162:1166	> 90%	1162:1166	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	14	69	theme	unaltered	2120:2128	arg1	matrix					2100:2105	the chitin matrix	2089:2105	the chitin matrix structurally unaltered	2089:2128	The removal of protein matrix induce a reduction of Young's modulus and maximum stress, leaving the chitin matrix structurally unaltered.					
32439612	0	70	theme	chitin-protein	60:73	arg1	composite					75:83	a natural chitin-protein composite	50:83	a natural chitin-protein composite	50:83	Influence of proteins on mechanical properties of a natural chitin-protein composite.					
32439612	1	71	theme	protein	175:181	arg1	fold					183:186	a protein fold	173:186	a protein fold	173:186	In many biogenic materials, chitin chains are assembled in fibrils that are wrapped by a protein fold.					
32439612	4	72	theme	chitin-protein	451:464	arg1	composites					466:475	chitin-protein composites	451:475	chitin-protein composites	451:475	Here, this research theme is studied using the pen of Loligo vulgaris as a model material of chitin-protein composites.					
32439612	9	73	theme	bridges	1378:1384	arg1	presence					1356:1363	the presence	1352:1363	the presence of disulfide bridges	1352:1384	These observations indicate that in the chitin matrix the proteins act as a strengthener, which efficacy is controlled by the presence of disulfide bridges.					
32439612	16	74	theme	di-sulfide	2348:2357	arg1	bridges					2359:2365	di-sulfide bridges	2348:2365	di-sulfide bridges that link crystalline chitin fibrils avoiding them to slide one on the other	2348:2442	In conclusion, this research shows that the proteins act as a stiff matrix reinforced by di-sulfide bridges that link crystalline chitin fibrils avoiding them to slide one on the other.					
32439612	11	75	theme	physio-chemical	1564:1578	arg1	properties					1580:1589	the physio-chemical properties	1560:1589	the physio-chemical properties of other biogenic polymeric composites	1560:1628	In conclusion, this knowledge can explain the physio-chemical properties of other biogenic polymeric composites and inspire the design of new materials.					
32439612	8	76	theme	disulfide	1000:1008	arg1	bonds					1010:1014	the disulfide bonds	996:1014	the disulfide bonds	996:1014	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	77	dep	relies	1046:1051	arg1	ii					1018:1019	ii	1018:1019	ii	1018:1019	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	14	78	theme	maximum	2065:2071	arg1	stress					2073:2078	maximum stress	2065:2078	maximum stress	2065:2078	The removal of protein matrix induce a reduction of Young's modulus and maximum stress, leaving the chitin matrix structurally unaltered.					
32439612	13	79	theme	maximum	1853:1859	arg1	stress					1861:1866	the maximum stress	1849:1866	the maximum stress	1849:1866	Here we show that the Young's modulus and the maximum stress mainly rely on protein disulfide bonds, the inter-proteins ones and those controlling the folding of chitin-binding domains.					
32439612	8	80	dep	stress	956:961	arg1	i					941:941	i	941:941	i	941:941	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	8	81	from	decrease	1147:1154	arg1	elongation					1218:1227	the maximum elongation	1206:1227	the maximum elongation	1206:1227	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	5	82	theme	proteic	554:560	arg1	phase					562:566	only the proteic phase	545:566	only the proteic phase	545:566	Chemical treatments were used to change the interactions involving only the proteic phase, through unfolding and/or degradation processes.					
32439612	8	83	dep	removal	1117:1123	arg1	iii					1102:1104	iii	1102:1104	iii	1102:1104	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	14	84	theme	Young	2045:2049	arg1	modulus					2053:2059	Young's modulus	2045:2059	Young's modulus	2045:2059	The removal of protein matrix induce a reduction of Young's modulus and maximum stress, leaving the chitin matrix structurally unaltered.					
32439612	7	85	theme	chemical	785:792	arg1	treatments					794:803	chemical treatments	785:803	chemical treatments	785:803	The data analysis showed that chemical treatments did not modify the structure of the chitin matrix.					
32439612	8	86	theme	test	899:902	arg1	analysis					904:911	the mechanical test analysis	884:911	the mechanical test analysis	884:911	This allowed to derive from the mechanical test analysis the following conclusions: (i) the maximum stress (σmax) relies on the presence of the disulfide bonds; (ii) the Young's modulus (E) relies on the overall correct folding of the proteins; (iii) the whole removal of proteins induces a decrease of E (> 90%) and σmax (> 80%), and an increase in the maximum elongation.					
32439612	6	87	theme	tensile	740:746	arg1	tests					748:752	tensile tests	740:752	tensile tests	740:752	Successively, structural and mechanical parameters were examined using spectroscopy, microscopy, X-ray diffractometry, and tensile tests.					
32439612	7	88	theme	data	759:762	arg1	analysis					764:771	The data analysis	755:771	The data analysis	755:771	The data analysis showed that chemical treatments did not modify the structure of the chitin matrix.					
32439612	5	89	dep	unfolding	577:585	arg1	processes					606:614	processes	606:614	processes	606:614	Chemical treatments were used to change the interactions involving only the proteic phase, through unfolding and/or degradation processes.					
32439612	11	90	theme	new	1656:1658	arg1	materials					1660:1668	new materials	1656:1668	new materials	1656:1668	In conclusion, this knowledge can explain the physio-chemical properties of other biogenic polymeric composites and inspire the design of new materials.					
32439612	13	91	theme	chitin-binding	1969:1982	arg1	domains					1984:1990	chitin-binding domains	1969:1990	chitin-binding domains	1969:1990	Here we show that the Young's modulus and the maximum stress mainly rely on protein disulfide bonds, the inter-proteins ones and those controlling the folding of chitin-binding domains.					
32439612	6	92	theme	X-ray	714:718	arg1	diffractometry					720:733	X-ray diffractometry	714:733	X-ray diffractometry	714:733	Successively, structural and mechanical parameters were examined using spectroscopy, microscopy, X-ray diffractometry, and tensile tests.					
34719741	5	0	theme	Sambaqui	801:808	arg1	soil					810:813	the Sambaqui soil	797:813	the Sambaqui soil	797:813	Using culture-dependent analysis we obtained few Streptomyces isolates from the Sambaqui soil.					
34719741	7	1	theme	carbon	1025:1030	arg1	sources					1032:1038	sole carbon sources	1020:1038	sole carbon sources	1020:1038	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	1	theme	carbon	1025:1030	arg1	polysaccharides					923:937	recalcitrant polysaccharides	910:937	recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose	910:1015	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	1	theme	carbon	1025:1030	arg1	chitin					949:954	chitin	949:954	chitin	949:954	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	1	theme	carbon	1025:1030	arg1	cellulose					1007:1015	microcrystalline cellulose	990:1015	microcrystalline cellulose	990:1015	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	1	theme	carbon	1025:1030	arg1	xylan					957:961	xylan	957:961	xylan	957:961	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	1	theme	carbon	1025:1030	arg1	carboxymethylcellulose					964:985	carboxymethylcellulose	964:985	carboxymethylcellulose	964:985	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	3	2	theme	different	501:509	arg1	layers					511:516	different layers	501:516	different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site	501:596	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	1	3	theme	biotechnological	249:264	arg1	potential					266:274	the biotechnological potential	245:274	the biotechnological potential	245:274	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	5	4	theme	culture-dependent	727:743	arg1	analysis					745:752	culture-dependent analysis	727:752	culture-dependent analysis	727:752	Using culture-dependent analysis we obtained few Streptomyces isolates from the Sambaqui soil.					
34719741	2	5	contain	had	343:345	arg2	impact					358:363	a profound impact	347:363	a profound impact	347:363	It has also had a profound impact on microbial taxonomy in the last decades.					
34719741	2	5	contain	had	343:345	arg1	It					331:332	It	331:332	It	331:332	It has also had a profound impact on microbial taxonomy in the last decades.					
34719741	6	6	theme	Streptomyces	843:854	arg1	sp					856:857	Streptomyces sp	843:857	Streptomyces sp	843:857	One of the isolates, named Streptomyces sp.					
34719741	2	7	theme	profound	349:356	arg1	impact					358:363	a profound impact	347:363	a profound impact	347:363	It has also had a profound impact on microbial taxonomy in the last decades.					
34719741	7	8	contain	containing	899:908	arg2	chitin					949:954	chitin	949:954	chitin	949:954	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	8	contain	containing	899:908	arg2	xylan					957:961	xylan	957:961	xylan	957:961	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	8	contain	containing	899:908	arg2	polysaccharides					923:937	recalcitrant polysaccharides	910:937	recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose	910:1015	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	8	contain	containing	899:908	arg2	carboxymethylcellulose					964:985	carboxymethylcellulose	964:985	carboxymethylcellulose	964:985	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	8	contain	containing	899:908	arg1	medium					892:897	minimal medium	884:897	minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources	884:1038	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	8	contain	containing	899:908	arg2	sources					1032:1038	sole carbon sources	1020:1038	sole carbon sources	1020:1038	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	8	contain	containing	899:908	arg2	cellulose					1007:1015	microcrystalline cellulose	990:1015	microcrystalline cellulose	990:1015	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	3	9	theme	shell	558:562	arg1	site					593:596	a shell mound Sambaqui archeological site	556:596	a shell mound Sambaqui archeological site	556:596	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	5	10	theme	few	766:768	arg1	isolates					783:790	few Streptomyces isolates	766:790	few Streptomyces isolates from the Sambaqui soil	766:813	Using culture-dependent analysis we obtained few Streptomyces isolates from the Sambaqui soil.					
34719741	9	11	theme	biosynthetic	1241:1252	arg1	clusters					1259:1266	biosynthetic gene clusters	1241:1266	not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites	1164:1362	The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.					
34719741	3	12	used	used	416:419	arg2	we					413:414	we	413:414	we	413:414	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	1	13	theme	prokaryotes	279:289	arg1	potential					266:274	the biotechnological potential	245:274	the biotechnological potential	245:274	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	1	13	theme	prokaryotes	279:289	arg1	diversity					231:239	diversity	231:239	diversity	231:239	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	0	14	theme	degrading	89:97	arg1	polysaccharides					112:126	Streptomyces degrading recalcitrant polysaccharides	76:126	Streptomyces degrading recalcitrant polysaccharides	76:126	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.					
34719741	6	15	theme	isolates	827:834	arg1	One					816:818	One	816:818	One	816:818	One of the isolates, named Streptomyces sp.					
34719741	6	15	theme	isolates	827:834	arg1	isolates					827:834	the isolates	823:834	the isolates	823:834	One of the isolates, named Streptomyces sp.					
34719741	3	16	theme	anthropogenic	524:536	arg1	soil					538:541	an anthropogenic soil	521:541	an anthropogenic soil collected at a shell mound Sambaqui archeological site	521:596	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	1	17	theme	traditional	168:178	arg1	techniques					193:202	traditional cell culture techniques	168:202	traditional cell culture techniques	168:202	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	0	18	theme	Streptomyces	76:87	arg1	polysaccharides					112:126	Streptomyces degrading recalcitrant polysaccharides	76:126	Streptomyces degrading recalcitrant polysaccharides	76:126	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.					
34719741	3	19	theme	soil	538:541	arg1	layers					511:516	different layers	501:516	different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site	501:596	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	1	20	theme	cell	180:183	arg1	techniques					193:202	traditional cell culture techniques	168:202	traditional cell culture techniques	168:202	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	9	21	theme	secondary	1342:1350	arg1	metabolites					1352:1362	biotechnologically interesting secondary metabolites	1311:1362	biotechnologically interesting secondary metabolites	1311:1362	The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.					
34719741	8	22	theme	free	1116:1119	arg1	supernatants					1121:1132	cell free supernatants	1111:1132	cell free supernatants	1111:1132	The activities of enzymes degrading these compounds were confirmed in cell free supernatants.					
34719741	3	23	theme	mound	564:568	arg1	site					593:596	a shell mound Sambaqui archeological site	556:596	a shell mound Sambaqui archeological site	556:596	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	9	24	theme	genome	1139:1144	arg1	sequence					1146:1153	The genome sequence	1135:1153	The genome sequence	1135:1153	The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.					
34719741	1	25	theme	culture	185:191	arg1	techniques					193:202	traditional cell culture techniques	168:202	traditional cell culture techniques	168:202	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	8	26	theme	cell	1111:1114	arg1	supernatants					1121:1132	cell free supernatants	1111:1132	cell free supernatants	1111:1132	The activities of enzymes degrading these compounds were confirmed in cell free supernatants.					
34719741	8	27	theme	enzymes	1059:1065	arg1	activities					1045:1054	The activities	1041:1054	The activities of enzymes degrading these compounds	1041:1091	The activities of enzymes degrading these compounds were confirmed in cell free supernatants.					
34719741	3	28	theme	Sambaqui	570:577	arg1	site					593:596	a shell mound Sambaqui archeological site	556:596	a shell mound Sambaqui archeological site	556:596	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	3	29	theme	metagenome	421:430	arg1	sequencing					450:459	metagenome 16S rDNA amplicon sequencing	421:459	metagenome 16S rDNA amplicon sequencing	421:459	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	1	30	theme	different	294:302	arg1	habitats					304:311	different habitats	294:311	different habitats around the world	294:328	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	0	31	theme	polysaccharides	112:126	arg1	source					66:71	a source	64:71	a source of Streptomyces degrading recalcitrant polysaccharides	64:126	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.					
34719741	7	32	theme	microcrystalline	990:1005	arg1	cellulose					1007:1015	microcrystalline cellulose	990:1015	microcrystalline cellulose	990:1015	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	0	33	theme	recalcitrant	99:110	arg1	polysaccharides					112:126	Streptomyces degrading recalcitrant polysaccharides	76:126	Streptomyces degrading recalcitrant polysaccharides	76:126	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.					
34719741	3	34	theme	layers	511:516	arg1	composition					486:496	the microbiome composition	471:496	the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site	471:596	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	1	35	theme	amplicon	140:147	arg1	sequencing					153:162	Metagenome amplicon DNA sequencing	129:162	Metagenome amplicon DNA sequencing	129:162	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	0	36	theme	mound	26:30	arg1	microbiome					4:13	The microbiome	0:13	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.	0:127	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.					
34719741	4	37	dep	phyla	651:655	arg1	Thaumarchaeota					705:718	Thaumarchaeota	705:718	Thaumarchaeota	705:718	The Samabaqui soil microbiome is mainly composed by phyla Acidobacteria, Rokubacteria, Proteobacteria and Thaumarchaeota.					
34719741	4	37	dep	phyla	651:655	arg1	Proteobacteria					686:699	Proteobacteria	686:699	Proteobacteria	686:699	The Samabaqui soil microbiome is mainly composed by phyla Acidobacteria, Rokubacteria, Proteobacteria and Thaumarchaeota.					
34719741	4	37	dep	phyla	651:655	arg1	phyla					651:655	phyla	651:655	phyla Acidobacteria, Rokubacteria, Proteobacteria and Thaumarchaeota	651:718	The Samabaqui soil microbiome is mainly composed by phyla Acidobacteria, Rokubacteria, Proteobacteria and Thaumarchaeota.					
34719741	4	37	dep	phyla	651:655	arg1	Rokubacteria					672:683	Rokubacteria	672:683	Rokubacteria	672:683	The Samabaqui soil microbiome is mainly composed by phyla Acidobacteria, Rokubacteria, Proteobacteria and Thaumarchaeota.					
34719741	4	37	dep	phyla	651:655	arg1	Acidobacteria					657:669	Acidobacteria	657:669	Acidobacteria	657:669	The Samabaqui soil microbiome is mainly composed by phyla Acidobacteria, Rokubacteria, Proteobacteria and Thaumarchaeota.					
34719741	3	38	theme	amplicon	441:448	arg1	sequencing					450:459	metagenome 16S rDNA amplicon sequencing	421:459	metagenome 16S rDNA amplicon sequencing	421:459	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	2	39	theme	last	394:397	arg1	decades					399:405	the last decades	390:405	the last decades	390:405	It has also had a profound impact on microbial taxonomy in the last decades.					
34719741	1	40	from	potential	266:274	arg1	habitats					304:311	different habitats	294:311	different habitats around the world	294:328	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	9	41	theme	gene	1254:1257	arg1	clusters					1259:1266	biosynthetic gene clusters	1241:1266	not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites	1164:1362	The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.					
34719741	0	42	theme	ancient	33:39	arg1	waste					55:59	ancient anthropogenic waste	33:59	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.	0:127	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.					
34719741	1	43	dep	diversity	231:239	arg1	the					227:229	the	227:229	the	227:229	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	3	44	theme	archeological	579:591	arg1	site					593:596	a shell mound Sambaqui archeological site	556:596	a shell mound Sambaqui archeological site	556:596	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	2	45	from	taxonomy	378:385	arg1	decades					399:405	the last decades	390:405	the last decades	390:405	It has also had a profound impact on microbial taxonomy in the last decades.					
34719741	4	46	theme	Samabaqui	603:611	arg1	microbiome					618:627	The Samabaqui soil microbiome	599:627	The Samabaqui soil microbiome	599:627	The Samabaqui soil microbiome is mainly composed by phyla Acidobacteria, Rokubacteria, Proteobacteria and Thaumarchaeota.					
34719741	3	47	theme	16S	432:434	arg1	sequencing					450:459	metagenome 16S rDNA amplicon sequencing	421:459	metagenome 16S rDNA amplicon sequencing	421:459	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	5	48	dep	obtained	757:764	arg1	Using					721:725	Using	721:725	Using culture-dependent analysis	721:752	Using culture-dependent analysis we obtained few Streptomyces isolates from the Sambaqui soil.					
34719741	5	49	from	soil	810:813	arg1	isolates					783:790	few Streptomyces isolates	766:790	few Streptomyces isolates from the Sambaqui soil	766:813	Using culture-dependent analysis we obtained few Streptomyces isolates from the Sambaqui soil.					
34719741	9	50	theme	genes	1187:1191	arg1	arsenal					1176:1182	an arsenal	1173:1182	not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites	1164:1362	The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.					
34719741	9	50	theme	genes	1187:1191	arg1	clusters					1259:1266	biosynthetic gene clusters	1241:1266	not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites	1164:1362	The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.					
34719741	9	51	theme	polysaccharides	1204:1218	arg1	degradation					1220:1230	polysaccharides degradation	1204:1230	polysaccharides degradation	1204:1230	The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.					
34719741	4	52	theme	soil	613:616	arg1	microbiome					618:627	The Samabaqui soil microbiome	599:627	The Samabaqui soil microbiome	599:627	The Samabaqui soil microbiome is mainly composed by phyla Acidobacteria, Rokubacteria, Proteobacteria and Thaumarchaeota.					
34719741	1	53	from	diversity	231:239	arg1	habitats					304:311	different habitats	294:311	different habitats around the world	294:328	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	9	54	theme	interesting	1330:1340	arg1	metabolites					1352:1362	biotechnologically interesting secondary metabolites	1311:1362	biotechnologically interesting secondary metabolites	1311:1362	The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.					
34719741	9	55	theme	related	1193:1199	arg1	genes					1187:1191	genes	1187:1191	genes related to polysaccharides degradation	1187:1230	The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.					
34719741	1	56	theme	Metagenome	129:138	arg1	sequencing					153:162	Metagenome amplicon DNA sequencing	129:162	Metagenome amplicon DNA sequencing	129:162	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	7	57	theme	recalcitrant	910:921	arg1	sources					1032:1038	sole carbon sources	1020:1038	sole carbon sources	1020:1038	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	57	theme	recalcitrant	910:921	arg1	polysaccharides					923:937	recalcitrant polysaccharides	910:937	recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose	910:1015	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	57	theme	recalcitrant	910:921	arg1	chitin					949:954	chitin	949:954	chitin	949:954	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	57	theme	recalcitrant	910:921	arg1	cellulose					1007:1015	microcrystalline cellulose	990:1015	microcrystalline cellulose	990:1015	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	57	theme	recalcitrant	910:921	arg1	xylan					957:961	xylan	957:961	xylan	957:961	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	57	theme	recalcitrant	910:921	arg1	carboxymethylcellulose					964:985	carboxymethylcellulose	964:985	carboxymethylcellulose	964:985	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	3	58	theme	rDNA	436:439	arg1	sequencing					450:459	metagenome 16S rDNA amplicon sequencing	421:459	metagenome 16S rDNA amplicon sequencing	421:459	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	7	59	theme	minimal	884:890	arg1	medium					892:897	minimal medium	884:897	minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources	884:1038	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	0	60	dep	microbiome	4:13	arg1	waste					55:59	ancient anthropogenic waste	33:59	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.	0:127	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.					
34719741	2	61	theme	microbial	368:376	arg1	taxonomy					378:385	microbial taxonomy	368:385	microbial taxonomy in the last decades	368:405	It has also had a profound impact on microbial taxonomy in the last decades.					
34719741	3	62	theme	microbiome	475:484	arg1	composition					486:496	the microbiome composition	471:496	the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site	471:596	Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site.					
34719741	0	63	theme	anthropogenic	41:53	arg1	waste					55:59	ancient anthropogenic waste	33:59	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.	0:127	The microbiome of a shell mound: ancient anthropogenic waste as a source of Streptomyces degrading recalcitrant polysaccharides.					
34719741	9	64	theme	metabolites	1352:1362	arg1	production					1297:1306	the production	1293:1306	the production of biotechnologically interesting secondary metabolites	1293:1362	The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.					
34719741	7	65	theme	sole	1020:1023	arg1	sources					1032:1038	sole carbon sources	1020:1038	sole carbon sources	1020:1038	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	65	theme	sole	1020:1023	arg1	polysaccharides					923:937	recalcitrant polysaccharides	910:937	recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose	910:1015	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	65	theme	sole	1020:1023	arg1	chitin					949:954	chitin	949:954	chitin	949:954	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	65	theme	sole	1020:1023	arg1	cellulose					1007:1015	microcrystalline cellulose	990:1015	microcrystalline cellulose	990:1015	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	65	theme	sole	1020:1023	arg1	xylan					957:961	xylan	957:961	xylan	957:961	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	7	65	theme	sole	1020:1023	arg1	carboxymethylcellulose					964:985	carboxymethylcellulose	964:985	carboxymethylcellulose	964:985	S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources.					
34719741	1	66	theme	DNA	149:151	arg1	sequencing					153:162	Metagenome amplicon DNA sequencing	129:162	Metagenome amplicon DNA sequencing	129:162	Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world.					
34719741	5	67	theme	Streptomyces	770:781	arg1	isolates					783:790	few Streptomyces isolates	766:790	few Streptomyces isolates from the Sambaqui soil	766:813	Using culture-dependent analysis we obtained few Streptomyces isolates from the Sambaqui soil.					
32479023	9	0	theme	hydroxyl	1118:1125	arg1	radical					1136:1142	hydroxyl and DPPH radical	1118:1142	radical	1136:1142	The variation also affects antioxidant and antihyperlipemia activities investigated using hydroxyl and DPPH radical scavenging assay, and in hyperlipemia mice.					
32479023	4	1	from	compositions	505:516	arg1	EPSs					521:524	EPSs	521:524	EPSs identified	521:535	The predominant carbohydrate compositions in EPSs identified were glucose and mannose.					
32479023	8	2	theme	carbon	940:945	arg1	source					947:952	carbon source	940:952	carbon source	940:952	Furthermore, thermogravimetric analysis (TGA) indicated the EPS with sucrose and glucose as carbon source showed different degradation behavior compared with the other three EPSs.					
32479023	1	3	theme	source	147:152	arg1	effects					129:135	The effects	125:135	The effects of carbon source on properties and bioactivities of exopolysaccharide (EPS) produced by Trametes ochracea	125:241	The effects of carbon source on properties and bioactivities of exopolysaccharide (EPS) produced by Trametes ochracea were investigated in this study.					
32479023	10	4	theme	radical	1257:1263	arg1	scavenging					1265:1274	DPPH radical scavenging	1252:1274	DPPH radical scavenging	1252:1274	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	8	5	theme	different	961:969	arg1	behavior					983:990	different degradation behavior	961:990	different degradation behavior	961:990	Furthermore, thermogravimetric analysis (TGA) indicated the EPS with sucrose and glucose as carbon source showed different degradation behavior compared with the other three EPSs.					
32479023	2	6	theme	carbon	345:350	arg1	sources					352:358	five different carbon sources	330:358	five different carbon sources	330:358	The results indicated that EPS production varied with five different carbon sources.					
32479023	10	7	theme	DPPH	1252:1255	arg1	scavenging					1265:1274	DPPH radical scavenging	1252:1274	DPPH radical scavenging	1252:1274	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	4	8	theme	carbohydrate	492:503	arg1	glucose					542:548	glucose	542:548	glucose	542:548	The predominant carbohydrate compositions in EPSs identified were glucose and mannose.					
32479023	4	8	theme	carbohydrate	492:503	arg1	compositions					505:516	The predominant carbohydrate compositions	476:516	The predominant carbohydrate compositions in EPSs identified	476:535	The predominant carbohydrate compositions in EPSs identified were glucose and mannose.					
32479023	8	9	dep	indicated	894:902	arg1	showed					954:959	showed	954:959	indicated the EPS with sucrose and glucose as carbon source showed different degradation behavior compared with the other three EPSs	894:1025	Furthermore, thermogravimetric analysis (TGA) indicated the EPS with sucrose and glucose as carbon source showed different degradation behavior compared with the other three EPSs.					
32479023	10	10	from	viewpoint	1232:1240	arg1	content					1363:1369	the relatively high glucose content	1335:1369	the relatively high glucose content in EPS	1335:1376	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	10	10	from	viewpoint	1232:1240	arg1	source					1216:1221	the best carbon source	1200:1221	the best carbon source from the viewpoint of OH and DPPH radical scavenging activities	1200:1285	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	10	10	from	viewpoint	1232:1240	arg1	Sucrose					1188:1194	Sucrose	1188:1194	Sucrose	1188:1194	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	10	10	from	viewpoint	1232:1240	arg1	activity					1309:1316	antihyperlipemia activity	1292:1316	antihyperlipemia activity	1292:1316	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	9	11	theme	antioxidant	1055:1065	arg1	activities					1088:1097	antioxidant and antihyperlipemia activities	1055:1097	activities	1088:1097	The variation also affects antioxidant and antihyperlipemia activities investigated using hydroxyl and DPPH radical scavenging assay, and in hyperlipemia mice.					
32479023	7	12	theme	particular	710:719	arg1	EPS					721:723	Each particular EPS	705:723	Each particular EPS	705:723	Each particular EPS possessed the specific bands at 808-809 cm-1 and 914-922 cm-1, indicating both α- and β-configurations of the sugar units.					
32479023	0	13	theme	Trametes	89:96	arg1	ochracea					98:105	Trametes ochracea	89:105	Trametes ochracea (Agaricomycetes)	89:122	Effect of Carbon Source on Properties and Bioactivities of Exopolysaccharide Produced by Trametes ochracea (Agaricomycetes).					
32479023	0	13	theme	Trametes	89:96	arg1	Agaricomycetes					108:121	Agaricomycetes	108:121	Agaricomycetes	108:121	Effect of Carbon Source on Properties and Bioactivities of Exopolysaccharide Produced by Trametes ochracea (Agaricomycetes).					
32479023	7	14	from	cm-1	782:785	arg1	bands					748:752	the specific bands	735:752	the specific bands at 808-809 cm-1 and 914-922 cm-1	735:785	Each particular EPS possessed the specific bands at 808-809 cm-1 and 914-922 cm-1, indicating both α- and β-configurations of the sugar units.					
32479023	8	15	theme	thermogravimetric	861:877	arg1	analysis					879:886	thermogravimetric analysis	861:886	thermogravimetric analysis (TGA)	861:892	Furthermore, thermogravimetric analysis (TGA) indicated the EPS with sucrose and glucose as carbon source showed different degradation behavior compared with the other three EPSs.					
32479023	8	15	theme	thermogravimetric	861:877	arg1	TGA					889:891	TGA	889:891	TGA	889:891	Furthermore, thermogravimetric analysis (TGA) indicated the EPS with sucrose and glucose as carbon source showed different degradation behavior compared with the other three EPSs.					
32479023	10	16	theme	activities	1276:1285	arg1	viewpoint					1232:1240	the viewpoint	1228:1240	the viewpoint of OH and DPPH radical scavenging activities	1228:1285	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	10	17	theme	antihyperlipemia	1292:1307	arg1	activity					1309:1316	antihyperlipemia activity	1292:1316	antihyperlipemia activity	1292:1316	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	5	18	theme	glucose	608:614	arg1	content					616:622	the highest glucose content	596:622	the highest glucose content	596:622	The EPS fermented by sucrose has the highest glucose content.					
32479023	5	19	theme	highest	600:606	arg1	content					616:622	the highest glucose content	596:622	the highest glucose content	596:622	The EPS fermented by sucrose has the highest glucose content.					
32479023	9	20	theme	antihyperlipemia	1071:1086	arg1	activities					1088:1097	antioxidant and antihyperlipemia activities	1055:1097	activities	1088:1097	The variation also affects antioxidant and antihyperlipemia activities investigated using hydroxyl and DPPH radical scavenging assay, and in hyperlipemia mice.					
32479023	10	21	theme	best	1204:1207	arg1	source					1216:1221	the best carbon source	1200:1221	the best carbon source from the viewpoint of OH and DPPH radical scavenging activities	1200:1285	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	10	21	theme	best	1204:1207	arg1	Sucrose					1188:1194	Sucrose	1188:1194	Sucrose	1188:1194	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	10	22	from	activity	1309:1316	arg1	EPS					1374:1376	EPS	1374:1376	EPS	1374:1376	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	7	23	theme	sugar	835:839	arg1	units					841:845	the sugar units	831:845	the sugar units	831:845	Each particular EPS possessed the specific bands at 808-809 cm-1 and 914-922 cm-1, indicating both α- and β-configurations of the sugar units.					
32479023	3	24	theme	suitable	421:428	arg1	source					437:442	the most suitable carbon source	412:442	the most suitable carbon source for biomass and EPS production	412:473	After a fermentation period of 8 days, sucrose was the most suitable carbon source for biomass and EPS production.					
32479023	3	24	theme	suitable	421:428	arg1	sucrose					400:406	sucrose	400:406	sucrose	400:406	After a fermentation period of 8 days, sucrose was the most suitable carbon source for biomass and EPS production.					
32479023	1	25	from	effects	129:135	arg1	bioactivities					172:184	bioactivities	172:184	bioactivities	172:184	The effects of carbon source on properties and bioactivities of exopolysaccharide (EPS) produced by Trametes ochracea were investigated in this study.					
32479023	1	25	from	effects	129:135	arg1	properties					157:166	properties	157:166	properties	157:166	The effects of carbon source on properties and bioactivities of exopolysaccharide (EPS) produced by Trametes ochracea were investigated in this study.					
32479023	7	26	theme	units	841:845	arg1	β-configurations					811:826	β-configurations	811:826	β-configurations of the sugar units	811:845	Each particular EPS possessed the specific bands at 808-809 cm-1 and 914-922 cm-1, indicating both α- and β-configurations of the sugar units.					
32479023	7	26	theme	units	841:845	arg1	α-					804:805	α-	804:805	α-	804:805	Each particular EPS possessed the specific bands at 808-809 cm-1 and 914-922 cm-1, indicating both α- and β-configurations of the sugar units.					
32479023	5	27	contain	has	592:594	arg1	EPS					567:569	The EPS	563:569	The EPS fermented by sucrose	563:590	The EPS fermented by sucrose has the highest glucose content.					
32479023	5	27	contain	has	592:594	arg2	content					616:622	the highest glucose content	596:622	the highest glucose content	596:622	The EPS fermented by sucrose has the highest glucose content.					
32479023	10	28	from	content	1363:1369	arg1	EPS					1374:1376	EPS	1374:1376	EPS	1374:1376	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	8	29	theme	other	1010:1014	arg1	EPSs					1022:1025	the other three EPSs	1006:1025	the other three EPSs	1006:1025	Furthermore, thermogravimetric analysis (TGA) indicated the EPS with sucrose and glucose as carbon source showed different degradation behavior compared with the other three EPSs.					
32479023	2	30	theme	EPS	303:305	arg1	production					307:316	EPS production	303:316	EPS production	303:316	The results indicated that EPS production varied with five different carbon sources.					
32479023	10	31	theme	glucose	1355:1361	arg1	content					1363:1369	the relatively high glucose content	1335:1369	the relatively high glucose content in EPS	1335:1376	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	0	32	theme	Source	17:22	arg1	Effect					0:5	Effect	0:5	Effect of Carbon Source on Properties and Bioactivities of Exopolysaccharide	0:75	Effect of Carbon Source on Properties and Bioactivities of Exopolysaccharide Produced by Trametes ochracea (Agaricomycetes).					
32479023	8	33	theme	degradation	971:981	arg1	behavior					983:990	different degradation behavior	961:990	different degradation behavior	961:990	Furthermore, thermogravimetric analysis (TGA) indicated the EPS with sucrose and glucose as carbon source showed different degradation behavior compared with the other three EPSs.					
32479023	6	34	from	groups	689:694	arg1	EPSs					699:702	EPSs	699:702	EPSs	699:702	Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs.					
32479023	6	35	theme	characteristic	674:687	arg1	groups					689:694	prominent characteristic groups	664:694	prominent characteristic groups in EPSs	664:702	Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs.					
32479023	1	36	theme	exopolysaccharide	189:205	arg1	bioactivities					172:184	bioactivities	172:184	bioactivities	172:184	The effects of carbon source on properties and bioactivities of exopolysaccharide (EPS) produced by Trametes ochracea were investigated in this study.					
32479023	1	36	theme	exopolysaccharide	189:205	arg1	properties					157:166	properties	157:166	properties	157:166	The effects of carbon source on properties and bioactivities of exopolysaccharide (EPS) produced by Trametes ochracea were investigated in this study.					
32479023	3	37	theme	fermentation	369:380	arg1	period					382:387	a fermentation period	367:387	a fermentation period of 8 days	367:397	After a fermentation period of 8 days, sucrose was the most suitable carbon source for biomass and EPS production.					
32479023	6	38	theme	prominent	664:672	arg1	groups					689:694	prominent characteristic groups	664:694	prominent characteristic groups in EPSs	664:702	Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs.					
32479023	9	39	theme	DPPH	1131:1134	arg1	radical					1136:1142	hydroxyl and DPPH radical	1118:1142	radical	1136:1142	The variation also affects antioxidant and antihyperlipemia activities investigated using hydroxyl and DPPH radical scavenging assay, and in hyperlipemia mice.					
32479023	3	40	theme	carbon	430:435	arg1	source					437:442	the most suitable carbon source	412:442	the most suitable carbon source for biomass and EPS production	412:473	After a fermentation period of 8 days, sucrose was the most suitable carbon source for biomass and EPS production.					
32479023	3	40	theme	carbon	430:435	arg1	sucrose					400:406	sucrose	400:406	sucrose	400:406	After a fermentation period of 8 days, sucrose was the most suitable carbon source for biomass and EPS production.					
32479023	7	41	theme	specific	739:746	arg1	bands					748:752	the specific bands	735:752	the specific bands at 808-809 cm-1 and 914-922 cm-1	735:785	Each particular EPS possessed the specific bands at 808-809 cm-1 and 914-922 cm-1, indicating both α- and β-configurations of the sugar units.					
32479023	9	42	theme	radical	1136:1142	arg1	assay					1155:1159	hydroxyl and DPPH radical scavenging assay	1118:1159	hydroxyl and DPPH radical scavenging assay	1118:1159	The variation also affects antioxidant and antihyperlipemia activities investigated using hydroxyl and DPPH radical scavenging assay, and in hyperlipemia mice.					
32479023	9	43	theme	scavenging	1144:1153	arg1	assay					1155:1159	hydroxyl and DPPH radical scavenging assay	1118:1159	hydroxyl and DPPH radical scavenging assay	1118:1159	The variation also affects antioxidant and antihyperlipemia activities investigated using hydroxyl and DPPH radical scavenging assay, and in hyperlipemia mice.					
32479023	2	44	theme	different	335:343	arg1	sources					352:358	five different carbon sources	330:358	five different carbon sources	330:358	The results indicated that EPS production varied with five different carbon sources.					
32479023	3	45	theme	days	394:397	arg1	period					382:387	a fermentation period	367:387	a fermentation period of 8 days	367:397	After a fermentation period of 8 days, sucrose was the most suitable carbon source for biomass and EPS production.					
32479023	7	46	contain	possessed	725:733	arg2	bands					748:752	the specific bands	735:752	the specific bands at 808-809 cm-1 and 914-922 cm-1	735:785	Each particular EPS possessed the specific bands at 808-809 cm-1 and 914-922 cm-1, indicating both α- and β-configurations of the sugar units.					
32479023	7	46	contain	possessed	725:733	arg1	EPS					721:723	Each particular EPS	705:723	Each particular EPS	705:723	Each particular EPS possessed the specific bands at 808-809 cm-1 and 914-922 cm-1, indicating both α- and β-configurations of the sugar units.					
32479023	10	47	theme	OH	1245:1246	arg1	activities					1276:1285	OH and DPPH radical scavenging activities	1245:1285	OH and DPPH radical scavenging activities	1245:1285	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	10	48	theme	high	1350:1353	arg1	content					1363:1369	the relatively high glucose content	1335:1369	the relatively high glucose content in EPS	1335:1376	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	6	49	theme	spectral	637:644	arg1	analysis					646:653	FT-IR spectral analysis	631:653	FT-IR spectral analysis	631:653	Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs.					
32479023	10	50	from	source	1216:1221	arg1	EPS					1374:1376	EPS	1374:1376	EPS	1374:1376	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	9	51	theme	hyperlipemia	1169:1180	arg1	mice					1182:1185	hyperlipemia mice	1169:1185	hyperlipemia mice	1169:1185	The variation also affects antioxidant and antihyperlipemia activities investigated using hydroxyl and DPPH radical scavenging assay, and in hyperlipemia mice.					
32479023	3	52	theme	EPS	460:462	arg1	production					464:473	EPS production	460:473	EPS production	460:473	After a fermentation period of 8 days, sucrose was the most suitable carbon source for biomass and EPS production.					
32479023	6	53	theme	FT-IR	631:635	arg1	analysis					646:653	FT-IR spectral analysis	631:653	FT-IR spectral analysis	631:653	Then, FT-IR spectral analysis revealed prominent characteristic groups in EPSs.					
32479023	0	54	theme	Exopolysaccharide	59:75	arg1	Properties					27:36	Properties	27:36	Properties	27:36	Effect of Carbon Source on Properties and Bioactivities of Exopolysaccharide Produced by Trametes ochracea (Agaricomycetes).					
32479023	0	54	theme	Exopolysaccharide	59:75	arg1	Bioactivities					42:54	Bioactivities	42:54	Bioactivities	42:54	Effect of Carbon Source on Properties and Bioactivities of Exopolysaccharide Produced by Trametes ochracea (Agaricomycetes).					
32479023	8	55	with	EPS	908:910	arg1	sucrose					917:923	sucrose	917:923	sucrose	917:923	Furthermore, thermogravimetric analysis (TGA) indicated the EPS with sucrose and glucose as carbon source showed different degradation behavior compared with the other three EPSs.					
32479023	8	55	with	EPS	908:910	arg1	glucose					929:935	glucose	929:935	glucose	929:935	Furthermore, thermogravimetric analysis (TGA) indicated the EPS with sucrose and glucose as carbon source showed different degradation behavior compared with the other three EPSs.					
32479023	1	56	theme	Trametes	225:232	arg1	ochracea					234:241	Trametes ochracea	225:241	Trametes ochracea	225:241	The effects of carbon source on properties and bioactivities of exopolysaccharide (EPS) produced by Trametes ochracea were investigated in this study.					
32479023	10	57	theme	carbon	1209:1214	arg1	source					1216:1221	the best carbon source	1200:1221	the best carbon source from the viewpoint of OH and DPPH radical scavenging activities	1200:1285	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	10	57	theme	carbon	1209:1214	arg1	Sucrose					1188:1194	Sucrose	1188:1194	Sucrose	1188:1194	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32479023	4	58	theme	predominant	480:490	arg1	glucose					542:548	glucose	542:548	glucose	542:548	The predominant carbohydrate compositions in EPSs identified were glucose and mannose.					
32479023	4	58	theme	predominant	480:490	arg1	compositions					505:516	The predominant carbohydrate compositions	476:516	The predominant carbohydrate compositions in EPSs identified	476:535	The predominant carbohydrate compositions in EPSs identified were glucose and mannose.					
32479023	0	59	theme	Carbon	10:15	arg1	Source					17:22	Carbon Source	10:22	Carbon Source	10:22	Effect of Carbon Source on Properties and Bioactivities of Exopolysaccharide Produced by Trametes ochracea (Agaricomycetes).					
32479023	0	60	from	Effect	0:5	arg1	Properties					27:36	Properties	27:36	Properties	27:36	Effect of Carbon Source on Properties and Bioactivities of Exopolysaccharide Produced by Trametes ochracea (Agaricomycetes).					
32479023	0	60	from	Effect	0:5	arg1	Bioactivities					42:54	Bioactivities	42:54	Bioactivities	42:54	Effect of Carbon Source on Properties and Bioactivities of Exopolysaccharide Produced by Trametes ochracea (Agaricomycetes).					
32479023	1	61	theme	carbon	140:145	arg1	source					147:152	carbon source	140:152	carbon source	140:152	The effects of carbon source on properties and bioactivities of exopolysaccharide (EPS) produced by Trametes ochracea were investigated in this study.					
32479023	10	62	theme	scavenging	1265:1274	arg1	activities					1276:1285	OH and DPPH radical scavenging activities	1245:1285	OH and DPPH radical scavenging activities	1245:1285	Sucrose was the best carbon source from the viewpoint of OH and DPPH radical scavenging activities, and antihyperlipemia activity, probably due to the relatively high glucose content in EPS.					
32220708	0	0	from	characteristics	40:54	arg1	okra					122:125	fresh okra	116:125	fresh okra (Abelmoschus esculentus (L.) Moench)	116:162	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	1	1	theme	sulfate	242:248	arg1	GNP					277:279	GNP	277:279	GNP	277:279	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	1	theme	sulfate	242:248	arg1	precipitation					262:274	gradient ammonium sulfate ((NH4)2SO4) precipitation	224:274	gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method	224:287	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	1	theme	sulfate	242:248	arg1	NH4					252:254	NH4	252:254	NH4	252:254	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	0	2	theme	polysaccharides	95:109	arg1	preparation					11:21	Innovative preparation	0:21	Innovative preparation	0:21	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	2	theme	polysaccharides	95:109	arg1	characteristics					40:54	physicochemical characteristics	24:54	physicochemical characteristics	24:54	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	2	theme	polysaccharides	95:109	arg1	properties					71:80	functional properties	60:80	functional properties	60:80	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	3	from	okra	122:125	arg1	polysaccharides					95:109	bioactive polysaccharides	85:109	bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench)	85:162	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	3	from	okra	122:125	arg1	preparation					11:21	Innovative preparation	0:21	Innovative preparation	0:21	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	3	from	okra	122:125	arg1	characteristics					40:54	physicochemical characteristics	24:54	physicochemical characteristics	24:54	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	3	from	okra	122:125	arg1	properties					71:80	functional properties	60:80	functional properties	60:80	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	4	4	theme	α-glucosidase	1109:1121	arg1	activities					1134:1143	α-amylase and α-glucosidase inhibitory activities	1095:1143	α-amylase and α-glucosidase inhibitory activities	1095:1143	Furthermore, OPS-50 exhibited stronger DPPH radical-scavenging ability, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities in vitro than OPS, OPS-30, and OPS-40.					
32220708	2	5	theme	%	704:704	arg1	saturations					679:689	saturations	679:689	saturations of 30% and 40% (NH4)2SO4, respectively	679:728	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	2	6	theme	carbohydrate	507:518	arg1	contents					554:561	higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents	479:561	higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents	479:561	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	0	7	dep	Abelmoschus	128:138	arg1	esculentus					140:149	Abelmoschus esculentus	128:149	Abelmoschus esculentus (L.) Moench	128:161	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	7	dep	Abelmoschus	128:138	arg1	Moench					156:161	Moench	156:161	Abelmoschus esculentus (L.) Moench	128:161	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	2	8	theme	molar	614:618	arg1	ratios					620:625	different molar ratios	604:625	different molar ratios	604:625	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	0	9	theme	fresh	116:120	arg1	okra					122:125	fresh okra	116:125	fresh okra (Abelmoschus esculentus (L.) Moench)	116:162	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	10	from	properties	71:80	arg1	okra					122:125	fresh okra	116:125	fresh okra (Abelmoschus esculentus (L.) Moench)	116:162	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	3	11	contain	had	738:740	arg2	lower					742:746	lower	742:746	lower	742:746	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	3	11	contain	had	738:740	arg1	OPS-50					731:736	OPS-50	731:736	OPS-50	731:736	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	3	11	contain	had	738:740	arg2	chain					814:818	more flexible chain	800:818	more flexible chain	800:818	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	3	11	contain	had	738:740	arg2	structure					851:859	relatively looser surface structure	825:859	relatively looser surface structure	825:859	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	2	12	theme	%	447:447	arg1	2SO4					454:457	50% (NH4)2SO4	445:457	50% (NH4)2SO4 after TPP	445:467	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	2	13	theme	uronic	533:538	arg1	acid					540:543	uronic acid	533:543	uronic acid (22.34%)	533:552	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	2	13	theme	uronic	533:538	arg1	%					551:551	22.34%	546:551	22.34%	546:551	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	2	14	theme	acid	540:543	arg1	contents					554:561	higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents	479:561	higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents	479:561	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	0	15	dep	okra	122:125	arg1	L.					152:153	L.	152:153	L.	152:153	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	15	dep	okra	122:125	arg1	Abelmoschus					128:138	Abelmoschus	128:138	Abelmoschus	128:138	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	2	16	theme	50	445:446	arg1	%					447:447	%	447:447	%	447:447	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	1	17	theme	separate	319:326	arg1	OPSs					355:358	OPSs	355:358	OPSs	355:358	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	17	theme	separate	319:326	arg1	polysaccharides					338:352	separate bioactive polysaccharides	319:352	separate bioactive polysaccharides (OPSs)	319:359	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	3	18	theme	decreased	939:947	arg1	viscosity					958:966	decreased apparent viscosity	939:966	decreased apparent viscosity	939:966	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	2	19	theme	same	568:571	arg1	composition					588:598	same monosaccharide composition	568:598	same monosaccharide composition	568:598	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	3	20	theme	weight-average	748:761	arg1	weight					773:778	weight-average molecular weight	748:778	lower weight-average molecular weight (3.65 × 106 g/mol)	742:797	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	3	20	theme	weight-average	748:761	arg1	g/mol					792:796	3.65 × 106 g/mol	781:796	3.65 × 106 g/mol	781:796	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	1	21	theme	bioactive	328:336	arg1	OPSs					355:358	OPSs	355:358	OPSs	355:358	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	21	theme	bioactive	328:336	arg1	polysaccharides					338:352	separate bioactive polysaccharides	319:352	separate bioactive polysaccharides (OPSs)	319:359	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	4	22	theme	inhibitory	1123:1132	arg1	activities					1134:1143	α-amylase and α-glucosidase inhibitory activities	1095:1143	α-amylase and α-glucosidase inhibitory activities	1095:1143	Furthermore, OPS-50 exhibited stronger DPPH radical-scavenging ability, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities in vitro than OPS, OPS-30, and OPS-40.					
32220708	3	23	theme	molecular	763:771	arg1	weight					773:778	weight-average molecular weight	748:778	lower weight-average molecular weight (3.65 × 106 g/mol)	742:797	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	3	23	theme	molecular	763:771	arg1	g/mol					792:796	3.65 × 106 g/mol	781:796	3.65 × 106 g/mol	781:796	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	0	24	theme	Innovative	0:9	arg1	preparation					11:21	Innovative preparation	0:21	Innovative preparation	0:21	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	24	theme	Innovative	0:9	arg1	characteristics					40:54	physicochemical characteristics	24:54	physicochemical characteristics	24:54	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	24	theme	Innovative	0:9	arg1	properties					71:80	functional properties	60:80	functional properties	60:80	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	4	25	theme	α-amylase	1095:1103	arg1	activities					1134:1143	α-amylase and α-glucosidase inhibitory activities	1095:1143	α-amylase and α-glucosidase inhibitory activities	1095:1143	Furthermore, OPS-50 exhibited stronger DPPH radical-scavenging ability, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities in vitro than OPS, OPS-30, and OPS-40.					
32220708	1	26	theme	three-phase	180:190	arg1	TPP					206:208	TPP	206:208	TPP	206:208	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	26	theme	three-phase	180:190	arg1	partitioning					192:203	three-phase partitioning	180:203	three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method	180:287	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	0	27	theme	physicochemical	24:38	arg1	preparation					11:21	Innovative preparation	0:21	Innovative preparation	0:21	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	27	theme	physicochemical	24:38	arg1	characteristics					40:54	physicochemical characteristics	24:54	physicochemical characteristics	24:54	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	1	28	theme	2SO4	256:259	arg1	GNP					277:279	GNP	277:279	GNP	277:279	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	28	theme	2SO4	256:259	arg1	precipitation					262:274	gradient ammonium sulfate ((NH4)2SO4) precipitation	224:274	gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method	224:287	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	28	theme	2SO4	256:259	arg1	NH4					252:254	NH4	252:254	NH4	252:254	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	2	29	dep	%	447:447	arg1	NH4					450:452	NH4	450:452	NH4	450:452	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	2	30	dep	%	696:696	arg1	2SO4					711:714	2SO4	711:714	2SO4	711:714	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	3	31	theme	dynamic	972:978	arg1	viscoelasticity					980:994	dynamic viscoelasticity	972:994	dynamic viscoelasticity	972:994	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	4	32	theme	stronger	1027:1034	arg1	ability					1060:1066	stronger DPPH radical-scavenging ability	1027:1066	stronger DPPH radical-scavenging ability	1027:1066	Furthermore, OPS-50 exhibited stronger DPPH radical-scavenging ability, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities in vitro than OPS, OPS-30, and OPS-40.					
32220708	1	33	theme	precipitation	262:274	arg1	method					282:287	gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method	224:287	gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method	224:287	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	2	34	theme	2SO4	454:457	arg1	saturation					431:440	a saturation	429:440	a saturation of 50% (NH4)2SO4 after TPP	429:467	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	2	35	theme	different	604:612	arg1	ratios					620:625	different molar ratios	604:625	different molar ratios	604:625	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	1	36	used	used	299:302	arg2	TPP					206:208	TPP	206:208	TPP	206:208	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	36	used	used	299:302	arg2	partitioning					192:203	three-phase partitioning	180:203	three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method	180:287	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	2	37	theme	yield	500:504	arg1	contents					554:561	higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents	479:561	higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents	479:561	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	3	38	theme	apparent	949:956	arg1	viscosity					958:966	decreased apparent viscosity	939:966	decreased apparent viscosity	939:966	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	4	39	theme	radical-scavenging	1041:1058	arg1	ability					1060:1066	stronger DPPH radical-scavenging ability	1027:1066	stronger DPPH radical-scavenging ability	1027:1066	Furthermore, OPS-50 exhibited stronger DPPH radical-scavenging ability, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities in vitro than OPS, OPS-30, and OPS-40.					
32220708	0	40	theme	functional	60:69	arg1	preparation					11:21	Innovative preparation	0:21	Innovative preparation	0:21	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	0	40	theme	functional	60:69	arg1	properties					71:80	functional properties	60:80	functional properties	60:80	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	2	41	theme	precipitation	486:498	arg1	yield					500:504	higher precipitation yield	479:504	higher precipitation yield	479:504	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	4	42	theme	antioxidant	1069:1079	arg1	capacity					1081:1088	antioxidant capacity	1069:1088	antioxidant capacity	1069:1088	Furthermore, OPS-50 exhibited stronger DPPH radical-scavenging ability, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities in vitro than OPS, OPS-30, and OPS-40.					
32220708	3	43	dep	lower	742:746	arg1	weight					773:778	weight-average molecular weight	748:778	lower weight-average molecular weight (3.65 × 106 g/mol)	742:797	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	3	43	dep	lower	742:746	arg1	g/mol					792:796	3.65 × 106 g/mol	781:796	3.65 × 106 g/mol	781:796	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	2	44	theme	higher	479:484	arg1	yield					500:504	higher precipitation yield	479:504	higher precipitation yield	479:504	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	1	45	theme	fresh	366:370	arg1	pods					377:380	fresh okra pods	366:380	fresh okra pods	366:380	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	2	46	theme	monosaccharide	573:586	arg1	composition					588:598	same monosaccharide composition	568:598	same monosaccharide composition	568:598	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	2	47	theme	%	696:696	arg1	saturations					679:689	saturations	679:689	saturations of 30% and 40% (NH4)2SO4, respectively	679:728	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	3	48	theme	flexible	805:812	arg1	chain					814:818	more flexible chain	800:818	more flexible chain	800:818	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	1	49	theme	okra	372:375	arg1	pods					377:380	fresh okra pods	366:380	fresh okra pods	366:380	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	3	50	theme	looser	836:841	arg1	structure					851:859	relatively looser surface structure	825:859	relatively looser surface structure	825:859	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	0	51	theme	bioactive	85:93	arg1	polysaccharides					95:109	bioactive polysaccharides	85:109	bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench)	85:162	Innovative preparation, physicochemical characteristics and functional properties of bioactive polysaccharides from fresh okra (Abelmoschus esculentus (L.) Moench).					
32220708	2	52	from	saturations	679:689	arg1	GNP					672:674	GNP	672:674	GNP at saturations of 30% and 40% (NH4)2SO4, respectively	672:728	Results showed that OPS-50 obtained by GNP at a saturation of 50% (NH4)2SO4 after TPP presented higher precipitation yield, carbohydrate (86.82%) and uronic acid (22.34%) contents with same monosaccharide composition and different molar ratios compared with OPS-30 and OPS-40 obtained via GNP at saturations of 30% and 40% (NH4)2SO4, respectively.					
32220708	3	53	theme	surface	843:849	arg1	structure					851:859	relatively looser surface structure	825:859	relatively looser surface structure	825:859	OPS-50 had lower weight-average molecular weight (3.65 × 106 g/mol), more flexible chain, and relatively looser surface structure compared with OPS-30, OPS-40, and the OPS obtained with TPP parallelized with decreased apparent viscosity and dynamic viscoelasticity.					
32220708	1	54	theme	gradient	224:231	arg1	GNP					277:279	GNP	277:279	GNP	277:279	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	54	theme	gradient	224:231	arg1	precipitation					262:274	gradient ammonium sulfate ((NH4)2SO4) precipitation	224:274	gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method	224:287	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	54	theme	gradient	224:231	arg1	NH4					252:254	NH4	252:254	NH4	252:254	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	55	from	pods	377:380	arg1	OPSs					355:358	OPSs	355:358	OPSs	355:358	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	55	from	pods	377:380	arg1	polysaccharides					338:352	separate bioactive polysaccharides	319:352	separate bioactive polysaccharides (OPSs)	319:359	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	55	from	pods	377:380	arg1	extract					307:313	extract	307:313	extract	307:313	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	4	56	theme	DPPH	1036:1039	arg1	ability					1060:1066	stronger DPPH radical-scavenging ability	1027:1066	stronger DPPH radical-scavenging ability	1027:1066	Furthermore, OPS-50 exhibited stronger DPPH radical-scavenging ability, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities in vitro than OPS, OPS-30, and OPS-40.					
32220708	1	57	theme	ammonium	233:240	arg1	GNP					277:279	GNP	277:279	GNP	277:279	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	57	theme	ammonium	233:240	arg1	precipitation					262:274	gradient ammonium sulfate ((NH4)2SO4) precipitation	224:274	gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method	224:287	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
32220708	1	57	theme	ammonium	233:240	arg1	NH4					252:254	NH4	252:254	NH4	252:254	In this study, three-phase partitioning (TPP) coupled with gradient ammonium sulfate ((NH4)2SO4) precipitation (GNP) method was first used to extract and separate bioactive polysaccharides (OPSs) from fresh okra pods.					
33471368	1	0	theme	Sargassum	173:181	arg1	fusiforme					183:191	Sargassum fusiforme	173:191	Sargassum fusiforme	173:191	Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme was purified by column DEAE-52 and Sephadex G-100 to yield four components, namely, ESFP1, ESFP2, ESFP3 and ESFP4.					
33471368	7	1	theme	uronic	1153:1158	arg1	acid					1160:1163	the uronic acid and sulfate contents	1149:1184	acid	1160:1163	The results revealed that the bioactivities of the polysaccharides are related to their molecular weight, and the uronic acid and sulfate contents.					
33471368	6	2	theme	immunomodulatory	838:853	arg1	effect					855:860	The immunomodulatory effect	834:860	The immunomodulatory effect of these four polysaccharides	834:890	The immunomodulatory effect of these four polysaccharides was demonstrated by their ability to promote proliferation, and to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model.					
33471368	2	3	theme	chemical	347:354	arg1	assay					368:372	chemical composition assay	347:372	chemical composition assay	347:372	These components were characterized by chemical composition assay, GC/MS, HPGPC, UV and FT-IR techniques.					
33471368	6	4	theme	RAW264.7	1023:1030	arg1	model					1032:1036	a macrophage RAW264.7 model	1010:1036	a macrophage RAW264.7 model	1010:1036	The immunomodulatory effect of these four polysaccharides was demonstrated by their ability to promote proliferation, and to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model.					
33471368	6	5	theme	macrophage	1012:1021	arg1	model					1032:1036	a macrophage RAW264.7 model	1010:1036	a macrophage RAW264.7 model	1010:1036	The immunomodulatory effect of these four polysaccharides was demonstrated by their ability to promote proliferation, and to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model.					
33471368	2	6	theme	composition	356:366	arg1	assay					368:372	chemical composition assay	347:372	chemical composition assay	347:372	These components were characterized by chemical composition assay, GC/MS, HPGPC, UV and FT-IR techniques.					
33471368	7	7	theme	molecular	1127:1135	arg1	weight					1137:1142	their molecular weight	1121:1142	their molecular weight	1121:1142	The results revealed that the bioactivities of the polysaccharides are related to their molecular weight, and the uronic acid and sulfate contents.					
33471368	4	8	theme	antioxidant	630:640	arg1	activities					642:651	good antioxidant activities	625:651	good antioxidant activities	625:651	The results suggested that all the four components possess good antioxidant activities.					
33471368	3	9	theme	scavenging	526:535	arg1	activity					537:544	their radical scavenging activity	512:544	their radical scavenging activity	512:544	The in vitro antioxidant activities of the four purified fractions were investigated by measuring their radical scavenging activity and reducing power.					
33471368	3	10	theme	in vitro	418:425	arg1	activities					439:448	The in vitro antioxidant activities	414:448	The in vitro antioxidant activities of the four purified fractions	414:479	The in vitro antioxidant activities of the four purified fractions were investigated by measuring their radical scavenging activity and reducing power.					
33471368	5	11	theme	reducing	818:825	arg1	power					827:831	the greatest ferric reducing power	798:831	the greatest ferric reducing power	798:831	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	1	12	theme	Enzymatic	102:110	arg1	hydrolysate					112:122	Enzymatic hydrolysate	102:122	Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme	102:191	Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme was purified by column DEAE-52 and Sephadex G-100 to yield four components, namely, ESFP1, ESFP2, ESFP3 and ESFP4.					
33471368	3	13	theme	antioxidant	427:437	arg1	activities					439:448	The in vitro antioxidant activities	414:448	The in vitro antioxidant activities of the four purified fractions	414:479	The in vitro antioxidant activities of the four purified fractions were investigated by measuring their radical scavenging activity and reducing power.					
33471368	7	14	theme	polysaccharides	1090:1104	arg1	bioactivities					1069:1081	the bioactivities	1065:1081	the bioactivities of the polysaccharides	1065:1104	The results revealed that the bioactivities of the polysaccharides are related to their molecular weight, and the uronic acid and sulfate contents.					
33471368	7	14	theme	polysaccharides	1090:1104	arg1	related					1110:1116	related	1110:1116	related	1110:1116	The results revealed that the bioactivities of the polysaccharides are related to their molecular weight, and the uronic acid and sulfate contents.					
33471368	1	15	theme	column	209:214	arg1	DEAE-52					216:222	column DEAE-52	209:222	column DEAE-52	209:222	Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme was purified by column DEAE-52 and Sephadex G-100 to yield four components, namely, ESFP1, ESFP2, ESFP3 and ESFP4.					
33471368	5	16	theme	strongest	697:705	arg1	radical					744:750	the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	693:750	the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	693:750	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	0	17	theme	Biological	17:26	arg1	Activities					28:37	Biological Activities	17:37	Biological Activities	17:37	Purification and Biological Activities of Enzymatically Degraded Sargassum fusiforme Polysaccharides.					
33471368	6	18	theme	NO	996:997	arg1	release					999:1005	NO release	996:1005	NO release	996:1005	The immunomodulatory effect of these four polysaccharides was demonstrated by their ability to promote proliferation, and to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model.					
33471368	5	19	theme	1,1-diphenyl-2-picrylhydrazyl	707:735	arg1	radical					744:750	the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	693:750	the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	693:750	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	1	20	theme	crude	131:135	arg1	SFP					153:155	the crude polysaccharide (SFP)	127:156	the crude polysaccharide (SFP) extracted from Sargassum fusiforme	127:191	Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme was purified by column DEAE-52 and Sephadex G-100 to yield four components, namely, ESFP1, ESFP2, ESFP3 and ESFP4.					
33471368	2	21	theme	FT-IR	396:400	arg1	techniques					402:411	FT-IR techniques	396:411	FT-IR techniques	396:411	These components were characterized by chemical composition assay, GC/MS, HPGPC, UV and FT-IR techniques.					
33471368	7	22	theme	sulfate	1169:1175	arg1	contents					1177:1184	the uronic acid and sulfate contents	1149:1184	contents	1177:1184	The results revealed that the bioactivities of the polysaccharides are related to their molecular weight, and the uronic acid and sulfate contents.					
33471368	1	23	theme	Sephadex	228:235	arg1	G-100					237:241	Sephadex G-100	228:241	Sephadex G-100	228:241	Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme was purified by column DEAE-52 and Sephadex G-100 to yield four components, namely, ESFP1, ESFP2, ESFP3 and ESFP4.					
33471368	5	24	theme	greatest	802:809	arg1	power					827:831	the greatest ferric reducing power	798:831	the greatest ferric reducing power	798:831	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	5	25	theme	DPPH	738:741	arg1	radical					744:750	the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	693:750	the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	693:750	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	5	26	theme	hydroxyl	756:763	arg1	activity					784:791	hydroxyl radical-scavenging activity	756:791	hydroxyl radical-scavenging activity	756:791	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	0	27	theme	Sargassum	65:73	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and Biological Activities of Enzymatically Degraded Sargassum fusiforme Polysaccharides.					
33471368	0	27	theme	Sargassum	65:73	arg1	Activities					28:37	Biological Activities	17:37	Biological Activities	17:37	Purification and Biological Activities of Enzymatically Degraded Sargassum fusiforme Polysaccharides.					
33471368	5	28	contain	possess	685:691	arg2	activity					784:791	hydroxyl radical-scavenging activity	756:791	hydroxyl radical-scavenging activity	756:791	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	5	28	contain	possess	685:691	arg1	ESFP1					666:670	ESFP1	666:670	ESFP1	666:670	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	5	28	contain	possess	685:691	arg2	power					827:831	the greatest ferric reducing power	798:831	the greatest ferric reducing power	798:831	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	5	28	contain	possess	685:691	arg2	radical					744:750	the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	693:750	the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	693:750	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	6	29	theme	polysaccharides	876:890	arg1	effect					855:860	The immunomodulatory effect	834:860	The immunomodulatory effect of these four polysaccharides	834:890	The immunomodulatory effect of these four polysaccharides was demonstrated by their ability to promote proliferation, and to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model.					
33471368	1	30	theme	polysaccharide	137:150	arg1	SFP					153:155	the crude polysaccharide (SFP)	127:156	the crude polysaccharide (SFP) extracted from Sargassum fusiforme	127:191	Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme was purified by column DEAE-52 and Sephadex G-100 to yield four components, namely, ESFP1, ESFP2, ESFP3 and ESFP4.					
33471368	0	31	theme	Degraded	56:63	arg1	Sargassum					65:73	Enzymatically Degraded Sargassum	42:73	Enzymatically Degraded Sargassum	42:73	Purification and Biological Activities of Enzymatically Degraded Sargassum fusiforme Polysaccharides.					
33471368	4	32	contain	possess	617:623	arg1	components					606:615	all the four components	593:615	all the four components	593:615	The results suggested that all the four components possess good antioxidant activities.					
33471368	4	32	contain	possess	617:623	arg2	activities					642:651	good antioxidant activities	625:651	good antioxidant activities	625:651	The results suggested that all the four components possess good antioxidant activities.					
33471368	1	33	theme	SFP	153:155	arg1	hydrolysate					112:122	Enzymatic hydrolysate	102:122	Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme	102:191	Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme was purified by column DEAE-52 and Sephadex G-100 to yield four components, namely, ESFP1, ESFP2, ESFP3 and ESFP4.					
33471368	6	34	dep	ability	918:924	arg1	enhance					959:965	enhance	959:965	to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model	956:1036	The immunomodulatory effect of these four polysaccharides was demonstrated by their ability to promote proliferation, and to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model.					
33471368	6	34	dep	ability	918:924	arg1	promote					929:935	promote	929:935	to promote proliferation	926:949	The immunomodulatory effect of these four polysaccharides was demonstrated by their ability to promote proliferation, and to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model.					
33471368	3	35	theme	fractions	471:479	arg1	activities					439:448	The in vitro antioxidant activities	414:448	The in vitro antioxidant activities of the four purified fractions	414:479	The in vitro antioxidant activities of the four purified fractions were investigated by measuring their radical scavenging activity and reducing power.					
33471368	3	36	theme	purified	462:469	arg1	fractions					471:479	the four purified fractions	453:479	the four purified fractions	453:479	The in vitro antioxidant activities of the four purified fractions were investigated by measuring their radical scavenging activity and reducing power.					
33471368	5	37	theme	radical-scavenging	765:782	arg1	activity					784:791	hydroxyl radical-scavenging activity	756:791	hydroxyl radical-scavenging activity	756:791	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	4	38	theme	good	625:628	arg1	activities					642:651	good antioxidant activities	625:651	good antioxidant activities	625:651	The results suggested that all the four components possess good antioxidant activities.					
33471368	5	39	theme	ferric	811:816	arg1	power					827:831	the greatest ferric reducing power	798:831	the greatest ferric reducing power	798:831	Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power.					
33471368	3	40	theme	radical	518:524	arg1	activity					537:544	their radical scavenging activity	512:544	their radical scavenging activity	512:544	The in vitro antioxidant activities of the four purified fractions were investigated by measuring their radical scavenging activity and reducing power.					
33471368	6	41	theme	phagocytic	972:981	arg1	activity					983:990	phagocytic activity	972:990	phagocytic activity	972:990	The immunomodulatory effect of these four polysaccharides was demonstrated by their ability to promote proliferation, and to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model.					
33484743	0	0	theme	cyanobacterial	68:81	arg1	EPS					83:85	cyanobacterial EPS	68:85	cyanobacterial EPS	68:85	New procedure for separation and analysis of the main components of cyanobacterial EPS.					
33484743	7	1	theme	batch	1073:1077	arg1	conditions					1093:1102	heterotrophic and mixotrophic batch and fed-batch conditions	1043:1102	heterotrophic and mixotrophic batch and fed-batch conditions	1043:1102	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days.					
33484743	1	2	theme	substances	154:163	arg1	matrix					120:125	a matrix	118:125	a matrix	118:125	Phototrophic biofilms produce a matrix of extracellular polymeric substances (EPS), which holds the cells together and functions inter alia as nutrient storage and protection layer.					
33484743	9	3	from	beginning	1477:1485	arg1	dominant					1461:1468	dominant	1461:1468	dominant	1461:1468	While the amount of proteins in the EPS increased during the cultivation period, carbohydrates and lipids were dominant in the beginning and decreased afterwards.					
33484743	1	4	theme	nutrient	231:238	arg1	storage					240:246	nutrient storage	231:246	nutrient storage	231:246	Phototrophic biofilms produce a matrix of extracellular polymeric substances (EPS), which holds the cells together and functions inter alia as nutrient storage and protection layer.					
33484743	4	5	theme	lipids	671:676	arg1	separation					572:581	separation	572:581	separation	572:581	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	4	5	theme	lipids	671:676	arg1	analysis					587:594	analysis	587:594	analysis	587:594	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	0	6	theme	EPS	83:85	arg1	components					54:63	the main components	45:63	the main components of cyanobacterial EPS	45:85	New procedure for separation and analysis of the main components of cyanobacterial EPS.					
33484743	9	7	theme	proteins	1370:1377	arg1	proteins					1370:1377	proteins	1370:1377	proteins	1370:1377	While the amount of proteins in the EPS increased during the cultivation period, carbohydrates and lipids were dominant in the beginning and decreased afterwards.					
33484743	9	7	theme	proteins	1370:1377	arg1	amount					1360:1365	the amount	1356:1365	the amount of proteins in the EPS	1356:1388	While the amount of proteins in the EPS increased during the cultivation period, carbohydrates and lipids were dominant in the beginning and decreased afterwards.					
33484743	6	8	from	composition	935:945	arg1	sample					968:973	only one sample	959:973	only one sample	959:973	Afterwards, the method was transferred to real EPS samples allowing a deeper insight in the composition of EPS from only one sample.					
33484743	6	9	theme	EPS	950:952	arg1	composition					935:945	the composition	931:945	the composition of EPS from only one sample	931:973	Afterwards, the method was transferred to real EPS samples allowing a deeper insight in the composition of EPS from only one sample.					
33484743	3	10	theme	rough	440:444	arg1	simplifications					446:460	rough simplifications	440:460	rough simplifications	440:460	Thus, rough simplifications are used and commonly one or at most two components of the EPS are examined.					
33484743	7	11	from	spec	1020:1023	arg1	EPS-extracts					995:1006	EPS-extracts	995:1006	EPS-extracts	995:1006	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days.					
33484743	7	11	from	spec	1020:1023	arg1	composition					980:990	The composition	976:990	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions,	976:1103	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days.					
33484743	4	12	theme	%	725:725	arg1	rates					705:709	recovery rates	696:709	recovery rates of nearly 100 %	696:725	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	8	13	theme	higher	1210:1215	arg1	proteins					1254:1261	proteins	1254:1261	proteins	1254:1261	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	13	theme	higher	1210:1215	arg1	lipids					1243:1248	lipids	1243:1248	lipids	1243:1248	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	13	theme	higher	1210:1215	arg1	carbohydrates					1228:1240	carbohydrates	1228:1240	carbohydrates	1228:1240	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	13	theme	higher	1210:1215	arg1	amounts					1217:1223	higher amounts	1210:1223	higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively	1210:1305	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	4	14	from	EPS	599:601	arg1	components					615:624	the main components	606:624	the main components	606:624	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	8	15	theme	mixotrophic	1179:1189	arg1	cultivation					1191:1201	mixotrophic cultivation	1179:1201	mixotrophic cultivation	1179:1201	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	7	16	theme	heterotrophic	1043:1055	arg1	conditions					1093:1102	heterotrophic and mixotrophic batch and fed-batch conditions	1043:1102	heterotrophic and mixotrophic batch and fed-batch conditions	1043:1102	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days.					
33484743	8	17	theme	fed-batch	1331:1339	arg1	culture					1341:1347	batch or fed-batch culture	1322:1347	batch or fed-batch culture	1322:1347	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	18	theme	heterotrophic	1268:1280	arg1	cultivation					1282:1292	heterotrophic cultivation	1268:1292	heterotrophic cultivation respectively	1268:1305	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	7	19	theme	fed-batch	1083:1091	arg1	conditions					1093:1102	heterotrophic and mixotrophic batch and fed-batch conditions	1043:1102	heterotrophic and mixotrophic batch and fed-batch conditions	1043:1102	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days.					
33484743	7	20	theme	cultivation	1127:1137	arg1	period					1139:1144	a cultivation period	1125:1144	a cultivation period of 14 days	1125:1155	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days.					
33484743	0	21	theme	New	0:2	arg1	procedure					4:12	New procedure	0:12	New procedure for separation and analysis of the main components of cyanobacterial EPS.	0:86	New procedure for separation and analysis of the main components of cyanobacterial EPS.					
33484743	1	22	theme	protection	252:261	arg1	layer					263:267	protection layer	252:267	protection layer	252:267	Phototrophic biofilms produce a matrix of extracellular polymeric substances (EPS), which holds the cells together and functions inter alia as nutrient storage and protection layer.					
33484743	3	23	theme	EPS	521:523	arg1	components					503:512	commonly one or at most two components	475:512	commonly one or at most two components of the EPS	475:523	Thus, rough simplifications are used and commonly one or at most two components of the EPS are examined.					
33484743	4	24	theme	EPS	599:601	arg1	polysaccharides					630:644	EPS in the main components (i) polysaccharides	599:644	EPS in the main components (i) polysaccharides	599:644	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	9	25	from	amount	1360:1365	arg1	EPS					1386:1388	the EPS	1382:1388	the EPS	1382:1388	While the amount of proteins in the EPS increased during the cultivation period, carbohydrates and lipids were dominant in the beginning and decreased afterwards.					
33484743	1	26	theme	Phototrophic	88:99	arg1	biofilms					101:108	Phototrophic biofilms	88:108	Phototrophic biofilms	88:108	Phototrophic biofilms produce a matrix of extracellular polymeric substances (EPS), which holds the cells together and functions inter alia as nutrient storage and protection layer.					
33484743	6	27	theme	deeper	913:918	arg1	insight					920:926	a deeper insight	911:926	a deeper insight	911:926	Afterwards, the method was transferred to real EPS samples allowing a deeper insight in the composition of EPS from only one sample.					
33484743	5	28	theme	real	809:812	arg1	EPS					814:816	real EPS	809:816	real EPS described in literature	809:840	The method was established with synthetic EPS, which based on the composition of real EPS described in literature.					
33484743	9	29	theme	cultivation	1411:1421	arg1	period					1423:1428	the cultivation period	1407:1428	the cultivation period	1407:1428	While the amount of proteins in the EPS increased during the cultivation period, carbohydrates and lipids were dominant in the beginning and decreased afterwards.					
33484743	8	30	theme	carbohydrates	1228:1240	arg1	proteins					1254:1261	proteins	1254:1261	proteins	1254:1261	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	30	theme	carbohydrates	1228:1240	arg1	lipids					1243:1248	lipids	1243:1248	lipids	1243:1248	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	30	theme	carbohydrates	1228:1240	arg1	carbohydrates					1228:1240	carbohydrates	1228:1240	carbohydrates	1228:1240	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	30	theme	carbohydrates	1228:1240	arg1	amounts					1217:1223	higher amounts	1210:1223	higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively	1210:1305	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	2	31	theme	hydrolysis	372:381	arg1	products					383:390	lysis and hydrolysis products	362:390	polysaccharides, proteins, lipids and nucleic acids as well as lysis and hydrolysis products which makes the composition very complex	299:431	EPS mainly consist of water, polysaccharides, proteins, lipids and nucleic acids as well as lysis and hydrolysis products which makes the composition very complex.					
33484743	7	32	theme	EPS-extracts	995:1006	arg1	composition					980:990	The composition	976:990	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions,	976:1103	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days.					
33484743	9	33	from	dominant	1461:1468	arg1	beginning					1477:1485	the beginning	1473:1485	the beginning	1473:1485	While the amount of proteins in the EPS increased during the cultivation period, carbohydrates and lipids were dominant in the beginning and decreased afterwards.					
33484743	4	34	theme	main	610:613	arg1	components					615:624	the main components	606:624	the main components	606:624	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	4	35	theme	recovery	696:703	arg1	rates					705:709	recovery rates	696:709	recovery rates of nearly 100 %	696:725	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	6	36	from	sample	968:973	arg1	EPS					950:952	EPS	950:952	EPS from only one sample	950:973	Afterwards, the method was transferred to real EPS samples allowing a deeper insight in the composition of EPS from only one sample.					
33484743	6	36	from	sample	968:973	arg1	composition					935:945	the composition	931:945	the composition of EPS from only one sample	931:973	Afterwards, the method was transferred to real EPS samples allowing a deeper insight in the composition of EPS from only one sample.					
33484743	2	37	theme	lysis	362:366	arg1	products					383:390	lysis and hydrolysis products	362:390	polysaccharides, proteins, lipids and nucleic acids as well as lysis and hydrolysis products which makes the composition very complex	299:431	EPS mainly consist of water, polysaccharides, proteins, lipids and nucleic acids as well as lysis and hydrolysis products which makes the composition very complex.					
33484743	2	38	theme	complex	425:431	arg1	composition					408:418	the composition	404:418	the composition very complex	404:431	EPS mainly consist of water, polysaccharides, proteins, lipids and nucleic acids as well as lysis and hydrolysis products which makes the composition very complex.					
33484743	5	39	theme	synthetic	760:768	arg1	EPS					770:772	synthetic EPS	760:772	synthetic EPS	760:772	The method was established with synthetic EPS, which based on the composition of real EPS described in literature.					
33484743	8	40	theme	batch	1322:1326	arg1	culture					1341:1347	batch or fed-batch culture	1322:1347	batch or fed-batch culture	1322:1347	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	4	41	dep	proteins	652:659	arg1	ii					648:649	ii	648:649	ii	648:649	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	5	42	theme	EPS	814:816	arg1	composition					794:804	the composition	790:804	the composition of real EPS described in literature	790:840	The method was established with synthetic EPS, which based on the composition of real EPS described in literature.					
33484743	8	43	theme	proteins	1254:1261	arg1	proteins					1254:1261	proteins	1254:1261	proteins	1254:1261	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	43	theme	proteins	1254:1261	arg1	lipids					1243:1248	lipids	1243:1248	lipids	1243:1248	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	43	theme	proteins	1254:1261	arg1	carbohydrates					1228:1240	carbohydrates	1228:1240	carbohydrates	1228:1240	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	43	theme	proteins	1254:1261	arg1	amounts					1217:1223	higher amounts	1210:1223	higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively	1210:1305	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	1	44	theme	extracellular	130:142	arg1	EPS					166:168	EPS	166:168	EPS	166:168	Phototrophic biofilms produce a matrix of extracellular polymeric substances (EPS), which holds the cells together and functions inter alia as nutrient storage and protection layer.					
33484743	1	44	theme	extracellular	130:142	arg1	substances					154:163	extracellular polymeric substances	130:163	extracellular polymeric substances (EPS)	130:169	Phototrophic biofilms produce a matrix of extracellular polymeric substances (EPS), which holds the cells together and functions inter alia as nutrient storage and protection layer.					
33484743	8	45	theme	lipids	1243:1248	arg1	proteins					1254:1261	proteins	1254:1261	proteins	1254:1261	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	45	theme	lipids	1243:1248	arg1	lipids					1243:1248	lipids	1243:1248	lipids	1243:1248	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	45	theme	lipids	1243:1248	arg1	carbohydrates					1228:1240	carbohydrates	1228:1240	carbohydrates	1228:1240	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	8	45	theme	lipids	1243:1248	arg1	amounts					1217:1223	higher amounts	1210:1223	higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively	1210:1305	It was observed that mixotrophic cultivation led to higher amounts of carbohydrates, lipids and proteins than heterotrophic cultivation respectively, regardless of batch or fed-batch culture.					
33484743	4	46	theme	proteins	652:659	arg1	separation					572:581	separation	572:581	separation	572:581	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	4	46	theme	proteins	652:659	arg1	analysis					587:594	analysis	587:594	analysis	587:594	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	6	47	theme	EPS	890:892	arg1	samples					894:900	real EPS samples	885:900	real EPS samples allowing a deeper insight in the composition of EPS from only one sample	885:973	Afterwards, the method was transferred to real EPS samples allowing a deeper insight in the composition of EPS from only one sample.					
33484743	3	48	used	used	466:469	arg2	simplifications					446:460	rough simplifications	440:460	rough simplifications	440:460	Thus, rough simplifications are used and commonly one or at most two components of the EPS are examined.					
33484743	4	49	theme	polysaccharides	630:644	arg1	separation					572:581	separation	572:581	separation	572:581	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	4	49	theme	polysaccharides	630:644	arg1	analysis					587:594	analysis	587:594	analysis	587:594	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	6	50	theme	real	885:888	arg1	samples					894:900	real EPS samples	885:900	real EPS samples allowing a deeper insight in the composition of EPS from only one sample	885:973	Afterwards, the method was transferred to real EPS samples allowing a deeper insight in the composition of EPS from only one sample.					
33484743	0	51	theme	components	54:63	arg1	analysis					33:40	analysis	33:40	analysis	33:40	New procedure for separation and analysis of the main components of cyanobacterial EPS.					
33484743	0	51	theme	components	54:63	arg1	separation					18:27	separation	18:27	separation	18:27	New procedure for separation and analysis of the main components of cyanobacterial EPS.					
33484743	7	52	theme	days	1152:1155	arg1	period					1139:1144	a cultivation period	1125:1144	a cultivation period of 14 days	1125:1155	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days.					
33484743	2	53	theme	nucleic	337:343	arg1	acids					345:349	nucleic acids	337:349	nucleic acids	337:349	EPS mainly consist of water, polysaccharides, proteins, lipids and nucleic acids as well as lysis and hydrolysis products which makes the composition very complex.					
33484743	4	54	theme	new	554:556	arg1	procedure					558:566	a new procedure	552:566	a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids	552:676	In this work a new procedure for separation and analysis of EPS in the main components (i) polysaccharides, (ii) proteins and (iii) lipids is presented with recovery rates of nearly 100 %.					
33484743	0	55	theme	main	49:52	arg1	components					54:63	the main components	45:63	the main components of cyanobacterial EPS	45:85	New procedure for separation and analysis of the main components of cyanobacterial EPS.					
33484743	7	56	from	composition	980:990	arg1	spec					1020:1023	spec	1020:1023	spec	1020:1023	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days.					
33484743	1	57	theme	polymeric	144:152	arg1	EPS					166:168	EPS	166:168	EPS	166:168	Phototrophic biofilms produce a matrix of extracellular polymeric substances (EPS), which holds the cells together and functions inter alia as nutrient storage and protection layer.					
33484743	1	57	theme	polymeric	144:152	arg1	substances					154:163	extracellular polymeric substances	130:163	extracellular polymeric substances (EPS)	130:169	Phototrophic biofilms produce a matrix of extracellular polymeric substances (EPS), which holds the cells together and functions inter alia as nutrient storage and protection layer.					
33484743	7	58	theme	mixotrophic	1061:1071	arg1	conditions					1093:1102	heterotrophic and mixotrophic batch and fed-batch conditions	1043:1102	heterotrophic and mixotrophic batch and fed-batch conditions	1043:1102	The composition of EPS-extracts from Nostoc spec, cultivated under heterotrophic and mixotrophic batch and fed-batch conditions, was analysed during a cultivation period of 14 days.					
34364068	0	0	theme	hemp	85:88	arg1	microcapsules					99:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	5	1	theme	oil/water	861:869	arg1	emulsion					887:894	an oil/water (O/W) composite emulsion	858:894	an oil/water (O/W) composite emulsion	858:894	Through fluorescence microscopy observation, HSO was found to be successfully embedded in the wall materials to form an oil/water (O/W) composite emulsion.					
34364068	1	2	theme	seed	243:246	arg1	oil					248:250	embedding hemp seed oil	228:250	embedding hemp seed oil	228:250	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	0	3	theme	embedded	76:83	arg1	microcapsules					99:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	6	4	theme	450 W.	1044:1049	arg1	Fourier					1051:1057	450 W. Fourier	1044:1057	450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules	1044:1300	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	6	5	theme	scanning	971:978	arg1	SEM					1001:1003	SEM	1001:1003	SEM	1001:1003	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	6	5	theme	scanning	971:978	arg1	microscopy					989:998	scanning electron microscopy	971:998	scanning electron microscopy (SEM)	971:1004	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	0	6	theme	oil	95:97	arg1	microcapsules					99:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	0	7	from	Effect	0:5	arg1	preparation					28:38	the preparation	24:38	the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	24:111	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	0	7	from	Effect	0:5	arg1	establishment					121:133	the establishment	117:133	the establishment of oxidation kinetics models	117:162	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	8	8	theme	HSO	1554:1556	arg1	microcapsules					1558:1570	HSO microcapsules	1554:1570	HSO microcapsules	1554:1570	The zero-order model of HSO microcapsules was found to have a higher degree of fit; after verification, the model can better reflect the quality changes of HSO microcapsules during storage.					
34364068	0	9	theme	seed	90:93	arg1	microcapsules					99:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	8	10	theme	fit	1477:1479	arg1	degree					1467:1472	a higher degree	1458:1472	a higher degree of fit	1458:1479	The zero-order model of HSO microcapsules was found to have a higher degree of fit; after verification, the model can better reflect the quality changes of HSO microcapsules during storage.					
34364068	4	11	theme	ultrasonic	719:728	arg1	treatment					730:738	the ultrasonic treatment	715:738	the ultrasonic treatment	715:738	The particle size of the composite emulsion after 450 W ultrasonic treatment was significantly lower than the particle size of the emulsion without the ultrasonic treatment.					
34364068	6	12	theme	ultrasonic	1118:1127	arg1	treatment					1129:1137	ultrasonic treatment	1118:1137	ultrasonic treatment	1118:1137	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	5	13	theme	O/W	872:874	arg1	emulsion					887:894	an oil/water (O/W) composite emulsion	858:894	an oil/water (O/W) composite emulsion	858:894	Through fluorescence microscopy observation, HSO was found to be successfully embedded in the wall materials to form an oil/water (O/W) composite emulsion.					
34364068	3	14	theme	ultrasonic	484:493	arg1	power					495:499	ultrasonic power	484:499	ultrasonic power	484:499	Studies have shown that ultrasonic power has a significant impact on the stability of composite emulsions.					
34364068	4	15	theme	450 W	617:621	arg1	treatment					634:642	450 W ultrasonic treatment	617:642	450 W ultrasonic treatment	617:642	The particle size of the composite emulsion after 450 W ultrasonic treatment was significantly lower than the particle size of the emulsion without the ultrasonic treatment.					
34364068	0	16	theme	microcapsules	99:111	arg1	preparation					28:38	the preparation	24:38	the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	24:111	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	0	16	theme	microcapsules	99:111	arg1	establishment					121:133	the establishment	117:133	the establishment of oxidation kinetics models	117:162	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	1	17	theme	wall	314:317	arg1	microcapsules					180:192	microcapsules	180:192	microcapsules	180:192	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	17	theme	wall	314:317	arg1	materials					319:327	wall materials	314:327	wall materials	314:327	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	2	18	dep	microstructure	368:381	arg1	the					364:366	the	364:366	the	364:366	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules was studied.					
34364068	8	19	theme	microcapsules	1426:1438	arg1	model					1413:1417	The zero-order model	1398:1417	The zero-order model of HSO microcapsules	1398:1438	The zero-order model of HSO microcapsules was found to have a higher degree of fit; after verification, the model can better reflect the quality changes of HSO microcapsules during storage.					
34364068	5	20	theme	wall	835:838	arg1	materials					840:848	the wall materials	831:848	the wall materials	831:848	Through fluorescence microscopy observation, HSO was found to be successfully embedded in the wall materials to form an oil/water (O/W) composite emulsion.					
34364068	4	21	theme	composite	592:600	arg1	emulsion					602:609	the composite emulsion	588:609	the composite emulsion	588:609	The particle size of the composite emulsion after 450 W ultrasonic treatment was significantly lower than the particle size of the emulsion without the ultrasonic treatment.					
34364068	4	22	theme	emulsion	698:705	arg1	size					686:689	the particle size	673:689	the particle size of the emulsion	673:705	The particle size of the composite emulsion after 450 W ultrasonic treatment was significantly lower than the particle size of the emulsion without the ultrasonic treatment.					
34364068	6	23	theme	embedding	1264:1272	arg1	effect					1274:1279	embedding effect	1264:1279	embedding effect	1264:1279	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	7	24	dep	HSO	1341:1343	arg1	microcapsules					1353:1365	microcapsules	1353:1365	microcapsules	1353:1365	Finally, oxidation kinetics models of HSO and HSO microcapsules were constructed and verified.					
34364068	6	25	dep	stability	1250:1258	arg1	the					1246:1248	the	1246:1248	the	1246:1248	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	6	26	theme	spherical	943:951	arg1	structure					953:961	a smooth spherical structure	934:961	a smooth spherical structure	934:961	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	6	27	dep	Fourier	1051:1057	arg1	transform					1059:1067	transform	1059:1067	transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules	1059:1300	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	6	28	theme	electron	980:987	arg1	SEM					1001:1003	SEM	1001:1003	SEM	1001:1003	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	6	28	theme	electron	980:987	arg1	microscopy					989:998	scanning electron microscopy	971:998	scanning electron microscopy (SEM)	971:1004	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	4	29	theme	ultrasonic	623:632	arg1	treatment					634:642	450 W ultrasonic treatment	617:642	450 W ultrasonic treatment	617:642	The particle size of the composite emulsion after 450 W ultrasonic treatment was significantly lower than the particle size of the emulsion without the ultrasonic treatment.					
34364068	7	30	theme	HSO	1349:1351	arg1	models					1331:1336	oxidation kinetics models	1312:1336	oxidation kinetics models of HSO and HSO microcapsules	1312:1365	Finally, oxidation kinetics models of HSO and HSO microcapsules were constructed and verified.					
34364068	6	31	theme	smooth	936:941	arg1	structure					953:961	a smooth spherical structure	934:961	a smooth spherical structure	934:961	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	3	32	theme	significant	507:517	arg1	impact					519:524	a significant impact	505:524	a significant impact on the stability of composite emulsions	505:564	Studies have shown that ultrasonic power has a significant impact on the stability of composite emulsions.					
34364068	6	33	theme	complex	1199:1205	arg1	bonding					1177:1183	covalent bonding	1168:1183	covalent bonding of the SPI-MD complex	1168:1205	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	6	34	theme	particle	1015:1022	arg1	size					1024:1027	the particle size	1011:1027	the particle size	1011:1027	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	0	35	theme	ultrasound	10:19	arg1	Effect					0:5	Effect	0:5	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.	0:163	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	1	36	theme	soy	263:265	arg1	SPI					284:286	SPI	284:286	SPI	284:286	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	36	theme	soy	263:265	arg1	isolate					275:281	soy protein isolate	263:281	soy protein isolate (SPI)	263:287	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	6	37	theme	microcapsules	1288:1300	arg1	stability					1250:1258	stability	1250:1258	stability	1250:1258	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	6	37	theme	microcapsules	1288:1300	arg1	effect					1274:1279	embedding effect	1264:1279	embedding effect	1264:1279	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	2	38	theme	composite	410:418	arg1	emulsion					420:427	the composite emulsion	406:427	the composite emulsion	406:427	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules was studied.					
34364068	7	39	theme	kinetics	1322:1329	arg1	models					1331:1336	oxidation kinetics models	1312:1336	oxidation kinetics models of HSO and HSO microcapsules	1312:1365	Finally, oxidation kinetics models of HSO and HSO microcapsules were constructed and verified.					
34364068	5	40	theme	fluorescence	749:760	arg1	microscopy					762:771	fluorescence microscopy	749:771	fluorescence microscopy observation	749:783	Through fluorescence microscopy observation, HSO was found to be successfully embedded in the wall materials to form an oil/water (O/W) composite emulsion.					
34364068	1	41	theme	protein	267:273	arg1	SPI					284:286	SPI	284:286	SPI	284:286	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	41	theme	protein	267:273	arg1	isolate					275:281	soy protein isolate	263:281	soy protein isolate (SPI)	263:287	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	0	42	theme	oxidation	138:146	arg1	models					157:162	oxidation kinetics models	138:162	oxidation kinetics models	138:162	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	4	43	theme	emulsion	602:609	arg1	lower					662:666	lower	662:666	lower	662:666	The particle size of the composite emulsion after 450 W ultrasonic treatment was significantly lower than the particle size of the emulsion without the ultrasonic treatment.					
34364068	4	43	theme	emulsion	602:609	arg1	size					580:583	The particle size	567:583	The particle size of the composite emulsion after 450 W ultrasonic treatment	567:642	The particle size of the composite emulsion after 450 W ultrasonic treatment was significantly lower than the particle size of the emulsion without the ultrasonic treatment.					
34364068	3	44	theme	composite	546:554	arg1	emulsions					556:564	composite emulsions	546:564	composite emulsions	546:564	Studies have shown that ultrasonic power has a significant impact on the stability of composite emulsions.					
34364068	2	45	theme	power	355:359	arg1	effect					334:339	The effect	330:339	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules	330:445	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules was studied.					
34364068	2	46	theme	emulsion	420:427	arg1	characteristics					387:401	characteristics	387:401	characteristics	387:401	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules was studied.					
34364068	2	46	theme	emulsion	420:427	arg1	microstructure					368:381	microstructure	368:381	microstructure	368:381	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules was studied.					
34364068	2	46	theme	emulsion	420:427	arg1	microcapsules					433:445	microcapsules	433:445	microcapsules	433:445	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules was studied.					
34364068	5	47	theme	microscopy	762:771	arg1	observation					773:783	fluorescence microscopy observation	749:783	fluorescence microscopy observation	749:783	Through fluorescence microscopy observation, HSO was found to be successfully embedded in the wall materials to form an oil/water (O/W) composite emulsion.					
34364068	8	48	theme	HSO	1422:1424	arg1	microcapsules					1426:1438	HSO microcapsules	1422:1438	HSO microcapsules	1422:1438	The zero-order model of HSO microcapsules was found to have a higher degree of fit; after verification, the model can better reflect the quality changes of HSO microcapsules during storage.					
34364068	3	49	theme	emulsions	556:564	arg1	stability					533:541	the stability	529:541	the stability of composite emulsions	529:564	Studies have shown that ultrasonic power has a significant impact on the stability of composite emulsions.					
34364068	5	50	from	embedded	819:826	arg1	materials					840:848	the wall materials	831:848	the wall materials	831:848	Through fluorescence microscopy observation, HSO was found to be successfully embedded in the wall materials to form an oil/water (O/W) composite emulsion.					
34364068	1	51	theme	spray	211:215	arg1	drying					217:222	spray drying	211:222	spray drying	211:222	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	51	theme	spray	211:215	arg1	HSO					253:255	HSO	253:255	HSO	253:255	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	0	52	theme	soy	43:45	arg1	microcapsules					99:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	6	53	theme	spray-dried	901:911	arg1	microcapsules					913:925	The spray-dried microcapsules	897:925	The spray-dried microcapsules	897:925	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	0	54	theme	models	157:162	arg1	preparation					28:38	the preparation	24:38	the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	24:111	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	0	54	theme	models	157:162	arg1	establishment					121:133	the establishment	117:133	the establishment of oxidation kinetics models	117:162	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	6	55	dep	infrared	1069:1076	arg1	FTIR					1079:1082	FTIR	1079:1082	FTIR	1079:1082	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	8	56	contain	have	1453:1456	arg2	degree					1467:1472	a higher degree	1458:1472	a higher degree of fit	1458:1479	The zero-order model of HSO microcapsules was found to have a higher degree of fit; after verification, the model can better reflect the quality changes of HSO microcapsules during storage.					
34364068	8	56	contain	have	1453:1456	arg1	model					1413:1417	The zero-order model	1398:1417	The zero-order model of HSO microcapsules	1398:1438	The zero-order model of HSO microcapsules was found to have a higher degree of fit; after verification, the model can better reflect the quality changes of HSO microcapsules during storage.					
34364068	4	57	theme	particle	677:684	arg1	size					686:689	the particle size	673:689	the particle size of the emulsion	673:705	The particle size of the composite emulsion after 450 W ultrasonic treatment was significantly lower than the particle size of the emulsion without the ultrasonic treatment.					
34364068	8	58	theme	higher	1460:1465	arg1	degree					1467:1472	a higher degree	1458:1472	a higher degree of fit	1458:1479	The zero-order model of HSO microcapsules was found to have a higher degree of fit; after verification, the model can better reflect the quality changes of HSO microcapsules during storage.					
34364068	6	59	theme	SPI-MD	1192:1197	arg1	complex					1199:1205	the SPI-MD complex	1188:1205	the SPI-MD complex	1188:1205	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	0	60	theme	kinetics	148:155	arg1	models					157:162	oxidation kinetics models	138:162	oxidation kinetics models	138:162	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	6	61	theme	certain	1212:1218	arg1	extent					1220:1225	a certain extent	1210:1225	a certain extent	1210:1225	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	8	62	theme	zero-order	1402:1411	arg1	model					1413:1417	The zero-order model	1398:1417	The zero-order model of HSO microcapsules	1398:1438	The zero-order model of HSO microcapsules was found to have a higher degree of fit; after verification, the model can better reflect the quality changes of HSO microcapsules during storage.					
34364068	3	63	contain	has	501:503	arg2	impact					519:524	a significant impact	505:524	a significant impact on the stability of composite emulsions	505:564	Studies have shown that ultrasonic power has a significant impact on the stability of composite emulsions.					
34364068	3	63	contain	has	501:503	arg1	power					495:499	ultrasonic power	484:499	ultrasonic power	484:499	Studies have shown that ultrasonic power has a significant impact on the stability of composite emulsions.					
34364068	8	64	theme	microcapsules	1558:1570	arg1	changes					1543:1549	the quality changes	1531:1549	the quality changes of HSO microcapsules	1531:1570	The zero-order model of HSO microcapsules was found to have a higher degree of fit; after verification, the model can better reflect the quality changes of HSO microcapsules during storage.					
34364068	7	65	theme	oxidation	1312:1320	arg1	models					1331:1336	oxidation kinetics models	1312:1336	oxidation kinetics models of HSO and HSO microcapsules	1312:1365	Finally, oxidation kinetics models of HSO and HSO microcapsules were constructed and verified.					
34364068	6	66	theme	bonding	1177:1183	arg1	degree					1158:1163	the degree	1154:1163	the degree of covalent bonding of the SPI-MD complex to a certain extent	1154:1225	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	1	67	with	oil	248:250	arg1	MD					307:308	MD	307:308	MD	307:308	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	67	with	oil	248:250	arg1	SPI					284:286	SPI	284:286	SPI	284:286	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	67	with	oil	248:250	arg1	maltodextrin					293:304	maltodextrin	293:304	maltodextrin (MD)	293:309	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	67	with	oil	248:250	arg1	isolate					275:281	soy protein isolate	263:281	soy protein isolate (SPI)	263:287	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	5	68	theme	composite	877:885	arg1	emulsion					887:894	an oil/water (O/W) composite emulsion	858:894	an oil/water (O/W) composite emulsion	858:894	Through fluorescence microscopy observation, HSO was found to be successfully embedded in the wall materials to form an oil/water (O/W) composite emulsion.					
34364068	3	69	from	impact	519:524	arg1	stability					533:541	the stability	529:541	the stability of composite emulsions	529:564	Studies have shown that ultrasonic power has a significant impact on the stability of composite emulsions.					
34364068	2	70	from	effect	334:339	arg1	characteristics					387:401	characteristics	387:401	characteristics	387:401	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules was studied.					
34364068	2	70	from	effect	334:339	arg1	microcapsules					433:445	microcapsules	433:445	microcapsules	433:445	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules was studied.					
34364068	2	70	from	effect	334:339	arg1	microstructure					368:381	microstructure	368:381	microstructure	368:381	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules was studied.					
34364068	6	71	theme	covalent	1168:1175	arg1	bonding					1177:1183	covalent bonding	1168:1183	covalent bonding of the SPI-MD complex	1168:1205	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	5	72	from	materials	840:848	arg1	embedded					819:826	embedded	819:826	embedded	819:826	Through fluorescence microscopy observation, HSO was found to be successfully embedded in the wall materials to form an oil/water (O/W) composite emulsion.					
34364068	7	73	theme	HSO	1341:1343	arg1	models					1331:1336	oxidation kinetics models	1312:1336	oxidation kinetics models of HSO and HSO microcapsules	1312:1365	Finally, oxidation kinetics models of HSO and HSO microcapsules were constructed and verified.					
34364068	8	74	theme	quality	1535:1541	arg1	changes					1543:1549	the quality changes	1531:1549	the quality changes of HSO microcapsules	1531:1570	The zero-order model of HSO microcapsules was found to have a higher degree of fit; after verification, the model can better reflect the quality changes of HSO microcapsules during storage.					
34364068	0	75	theme	isolate-maltodextrin	55:74	arg1	microcapsules					99:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	2	76	theme	ultrasonic	344:353	arg1	power					355:359	ultrasonic power	344:359	ultrasonic power	344:359	The effect of ultrasonic power on the microstructure and characteristics of the composite emulsion and microcapsules was studied.					
34364068	6	77	dep	spectroscopy	1085:1096	arg1	found					1107:1111	found	1107:1111	found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules	1107:1300	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	6	78	theme	infrared	1069:1076	arg1	spectroscopy					1085:1096	infrared (FTIR) spectroscopy	1069:1096	infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules	1069:1300	The spray-dried microcapsules showed a smooth spherical structure through scanning electron microscopy (SEM), and the particle size was 10.7 μm at 450 W. Fourier transform infrared (FTIR) spectroscopy analysis found that ultrasonic treatment would increase the degree of covalent bonding of the SPI-MD complex to a certain extent, thereby improving the stability and embedding effect of the microcapsules.					
34364068	1	79	theme	embedding	228:236	arg1	oil					248:250	embedding hemp seed oil	228:250	embedding hemp seed oil	228:250	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	0	80	theme	protein	47:53	arg1	microcapsules					99:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	soy protein isolate-maltodextrin embedded hemp seed oil microcapsules	43:111	Effect of ultrasound on the preparation of soy protein isolate-maltodextrin embedded hemp seed oil microcapsules and the establishment of oxidation kinetics models.					
34364068	4	81	theme	particle	571:578	arg1	lower					662:666	lower	662:666	lower	662:666	The particle size of the composite emulsion after 450 W ultrasonic treatment was significantly lower than the particle size of the emulsion without the ultrasonic treatment.					
34364068	4	81	theme	particle	571:578	arg1	size					580:583	The particle size	567:583	The particle size of the composite emulsion after 450 W ultrasonic treatment	567:642	The particle size of the composite emulsion after 450 W ultrasonic treatment was significantly lower than the particle size of the emulsion without the ultrasonic treatment.					
34364068	1	82	with	drying	217:222	arg1	MD					307:308	MD	307:308	MD	307:308	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	82	with	drying	217:222	arg1	SPI					284:286	SPI	284:286	SPI	284:286	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	82	with	drying	217:222	arg1	maltodextrin					293:304	maltodextrin	293:304	maltodextrin (MD)	293:309	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	82	with	drying	217:222	arg1	isolate					275:281	soy protein isolate	263:281	soy protein isolate (SPI)	263:287	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364068	1	83	theme	hemp	238:241	arg1	oil					248:250	embedding hemp seed oil	228:250	embedding hemp seed oil	228:250	In this study, microcapsules were prepared by spray drying and embedding hemp seed oil (HSO) with soy protein isolate (SPI) and maltodextrin (MD) as wall materials.					
34364593	0	0	theme	able	75:78	arg1	unit					70:73	the smallest active unit	50:73	the smallest active unit able to induce an oxidative burst in dicot cells	50:122	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	0	0	theme	able	75:78	arg1	acid					32:35	The non-sulfated ulvanobiuronic acid	0:35	The non-sulfated ulvanobiuronic acid of ulvans	0:45	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	7	1	theme	dimers	946:951	arg1	activity					924:931	The elicitor activity	911:931	The elicitor activity of the ulvan dimers	911:951	The elicitor activity of the ulvan dimers did not require sulfation.					
34364593	6	2	theme	oxidative	879:887	arg1	burst					889:893	an NADPH oxidase-dependent oxidative burst	852:893	an NADPH oxidase-dependent oxidative burst	852:893	Both the polymer and the oligomer fractions induced an NADPH oxidase-dependent oxidative burst in plant cells.					
34364593	1	3	theme	green	137:141	arg1	algae					143:147	green algae	137:147	green algae	137:147	Ulvans from green algae are promising compounds for plant protection because they are environmentally friendly and induce plant defense responses.					
34364593	5	4	theme	de-sulfated	774:784	arg1	dimers					792:797	mostly sulfated and de-sulfated ulvan dimers	754:797	mostly sulfated and de-sulfated ulvan dimers	754:797	The oligomeric fractions were analyzed revealing mostly sulfated and de-sulfated ulvan dimers.					
34364593	6	5	theme	oxidase-dependent	861:877	arg1	burst					889:893	an NADPH oxidase-dependent oxidative burst	852:893	an NADPH oxidase-dependent oxidative burst	852:893	Both the polymer and the oligomer fractions induced an NADPH oxidase-dependent oxidative burst in plant cells.					
34364593	1	6	theme	plant	247:251	arg1	responses					261:269	plant defense responses	247:269	plant defense responses	247:269	Ulvans from green algae are promising compounds for plant protection because they are environmentally friendly and induce plant defense responses.					
34364593	5	7	theme	ulvan	786:790	arg1	dimers					792:797	mostly sulfated and de-sulfated ulvan dimers	754:797	mostly sulfated and de-sulfated ulvan dimers	754:797	The oligomeric fractions were analyzed revealing mostly sulfated and de-sulfated ulvan dimers.					
34364593	7	8	theme	elicitor	915:922	arg1	activity					924:931	The elicitor activity	911:931	The elicitor activity of the ulvan dimers	911:951	The elicitor activity of the ulvan dimers did not require sulfation.					
34364593	1	9	theme	defense	253:259	arg1	responses					261:269	plant defense responses	247:269	plant defense responses	247:269	Ulvans from green algae are promising compounds for plant protection because they are environmentally friendly and induce plant defense responses.					
34364593	0	10	theme	oxidative	93:101	arg1	burst					103:107	an oxidative burst	90:107	an oxidative burst	90:107	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	1	11	theme	plant	177:181	arg1	protection					183:192	plant protection	177:192	plant protection	177:192	Ulvans from green algae are promising compounds for plant protection because they are environmentally friendly and induce plant defense responses.					
34364593	1	12	theme	promising	153:161	arg1	Ulvans					125:130	Ulvans	125:130	Ulvans from green algae	125:147	Ulvans from green algae are promising compounds for plant protection because they are environmentally friendly and induce plant defense responses.					
34364593	1	12	theme	promising	153:161	arg1	compounds					163:171	promising compounds	153:171	promising compounds for plant protection	153:192	Ulvans from green algae are promising compounds for plant protection because they are environmentally friendly and induce plant defense responses.					
34364593	4	13	theme	acid	618:621	arg1	hydrolysis					623:632	acid hydrolysis	618:632	acid hydrolysis	618:632	The polymer was partially depolymerized using acid hydrolysis, and the oligomers were separated using size exclusion chromatography.					
34364593	8	14	theme	important	1051:1059	arg1	step					1066:1069	an important next step	1048:1069	an important next step	1048:1069	By identifying the smallest elicitor-active unit, HexA-Rha, we took an important next step to understand how the structure influences ulvan elicitor responses.					
34364593	3	15	theme	molecular	556:564	arg1	mass					566:569	molecular mass	556:569	molecular mass	556:569	The polysaccharide from Ulva fasciata was characterized regarding its monosaccharide composition, degree of sulfation, and molecular mass.					
34364593	4	16	theme	exclusion	679:687	arg1	chromatography					689:702	size exclusion chromatography	674:702	size exclusion chromatography	674:702	The polymer was partially depolymerized using acid hydrolysis, and the oligomers were separated using size exclusion chromatography.					
34364593	0	17	theme	non-sulfated	4:15	arg1	unit					70:73	the smallest active unit	50:73	the smallest active unit able to induce an oxidative burst in dicot cells	50:122	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	0	17	theme	non-sulfated	4:15	arg1	acid					32:35	The non-sulfated ulvanobiuronic acid	0:35	The non-sulfated ulvanobiuronic acid of ulvans	0:45	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	8	18	theme	elicitor	1120:1127	arg1	responses					1129:1137	ulvan elicitor responses	1114:1137	ulvan elicitor responses	1114:1137	By identifying the smallest elicitor-active unit, HexA-Rha, we took an important next step to understand how the structure influences ulvan elicitor responses.					
34364593	9	19	theme	desulfated	1144:1153	arg1	dimer					1161:1165	The desulfated ulvan dimer	1140:1165	The desulfated ulvan dimer	1140:1165	The desulfated ulvan dimer is discussed as a promising agro-biologic for sustainable agriculture.					
34364593	9	19	theme	desulfated	1144:1153	arg1	agro-biologic					1195:1207	a promising agro-biologic	1183:1207	a promising agro-biologic for sustainable agriculture	1183:1235	The desulfated ulvan dimer is discussed as a promising agro-biologic for sustainable agriculture.					
34364593	2	20	theme	suspension-cultured	383:401	arg1	cells					403:407	suspension-cultured cells	383:407	suspension-cultured cells of three dicot species	383:430	We analyzed the structure-function relationship of ulvan polymers and oligomers for their elicitor activity in suspension-cultured cells of three dicot species.					
34364593	8	21	theme	ulvan	1114:1118	arg1	responses					1129:1137	ulvan elicitor responses	1114:1137	ulvan elicitor responses	1114:1137	By identifying the smallest elicitor-active unit, HexA-Rha, we took an important next step to understand how the structure influences ulvan elicitor responses.					
34364593	6	22	theme	oligomer	825:832	arg1	fractions					834:842	the oligomer fractions	821:842	the oligomer fractions	821:842	Both the polymer and the oligomer fractions induced an NADPH oxidase-dependent oxidative burst in plant cells.					
34364593	9	23	theme	ulvan	1155:1159	arg1	dimer					1161:1165	The desulfated ulvan dimer	1140:1165	The desulfated ulvan dimer	1140:1165	The desulfated ulvan dimer is discussed as a promising agro-biologic for sustainable agriculture.					
34364593	9	23	theme	ulvan	1155:1159	arg1	agro-biologic					1195:1207	a promising agro-biologic	1183:1207	a promising agro-biologic for sustainable agriculture	1183:1235	The desulfated ulvan dimer is discussed as a promising agro-biologic for sustainable agriculture.					
34364593	8	24	theme	next	1061:1064	arg1	step					1066:1069	an important next step	1048:1069	an important next step	1048:1069	By identifying the smallest elicitor-active unit, HexA-Rha, we took an important next step to understand how the structure influences ulvan elicitor responses.					
34364593	2	25	theme	structure-function	288:305	arg1	relationship					307:318	the structure-function relationship	284:318	the structure-function relationship of ulvan polymers and oligomers for their elicitor activity	284:378	We analyzed the structure-function relationship of ulvan polymers and oligomers for their elicitor activity in suspension-cultured cells of three dicot species.					
34364593	0	26	theme	ulvanobiuronic	17:30	arg1	unit					70:73	the smallest active unit	50:73	the smallest active unit able to induce an oxidative burst in dicot cells	50:122	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	0	26	theme	ulvanobiuronic	17:30	arg1	acid					32:35	The non-sulfated ulvanobiuronic acid	0:35	The non-sulfated ulvanobiuronic acid of ulvans	0:45	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	2	27	theme	species	424:430	arg1	cells					403:407	suspension-cultured cells	383:407	suspension-cultured cells of three dicot species	383:430	We analyzed the structure-function relationship of ulvan polymers and oligomers for their elicitor activity in suspension-cultured cells of three dicot species.					
34364593	0	28	theme	ulvans	40:45	arg1	unit					70:73	the smallest active unit	50:73	the smallest active unit able to induce an oxidative burst in dicot cells	50:122	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	0	28	theme	ulvans	40:45	arg1	acid					32:35	The non-sulfated ulvanobiuronic acid	0:35	The non-sulfated ulvanobiuronic acid of ulvans	0:45	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	5	29	theme	sulfated	761:768	arg1	dimers					792:797	mostly sulfated and de-sulfated ulvan dimers	754:797	mostly sulfated and de-sulfated ulvan dimers	754:797	The oligomeric fractions were analyzed revealing mostly sulfated and de-sulfated ulvan dimers.					
34364593	4	30	theme	size	674:677	arg1	chromatography					689:702	size exclusion chromatography	674:702	size exclusion chromatography	674:702	The polymer was partially depolymerized using acid hydrolysis, and the oligomers were separated using size exclusion chromatography.					
34364593	2	31	theme	dicot	418:422	arg1	species					424:430	three dicot species	412:430	three dicot species	412:430	We analyzed the structure-function relationship of ulvan polymers and oligomers for their elicitor activity in suspension-cultured cells of three dicot species.					
34364593	0	32	theme	dicot	112:116	arg1	cells					118:122	dicot cells	112:122	dicot cells	112:122	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	3	33	from	fasciata	462:469	arg1	polysaccharide					437:450	The polysaccharide	433:450	The polysaccharide from Ulva fasciata	433:469	The polysaccharide from Ulva fasciata was characterized regarding its monosaccharide composition, degree of sulfation, and molecular mass.					
34364593	9	34	theme	sustainable	1213:1223	arg1	agriculture					1225:1235	sustainable agriculture	1213:1235	sustainable agriculture	1213:1235	The desulfated ulvan dimer is discussed as a promising agro-biologic for sustainable agriculture.					
34364593	5	35	theme	oligomeric	709:718	arg1	fractions					720:728	The oligomeric fractions	705:728	The oligomeric fractions	705:728	The oligomeric fractions were analyzed revealing mostly sulfated and de-sulfated ulvan dimers.					
34364593	2	36	theme	oligomers	342:350	arg1	relationship					307:318	the structure-function relationship	284:318	the structure-function relationship of ulvan polymers and oligomers for their elicitor activity	284:378	We analyzed the structure-function relationship of ulvan polymers and oligomers for their elicitor activity in suspension-cultured cells of three dicot species.					
34364593	9	37	theme	promising	1185:1193	arg1	dimer					1161:1165	The desulfated ulvan dimer	1140:1165	The desulfated ulvan dimer	1140:1165	The desulfated ulvan dimer is discussed as a promising agro-biologic for sustainable agriculture.					
34364593	9	37	theme	promising	1185:1193	arg1	agro-biologic					1195:1207	a promising agro-biologic	1183:1207	a promising agro-biologic for sustainable agriculture	1183:1235	The desulfated ulvan dimer is discussed as a promising agro-biologic for sustainable agriculture.					
34364593	6	38	theme	plant	898:902	arg1	cells					904:908	plant cells	898:908	plant cells	898:908	Both the polymer and the oligomer fractions induced an NADPH oxidase-dependent oxidative burst in plant cells.					
34364593	2	39	theme	polymers	329:336	arg1	relationship					307:318	the structure-function relationship	284:318	the structure-function relationship of ulvan polymers and oligomers for their elicitor activity	284:378	We analyzed the structure-function relationship of ulvan polymers and oligomers for their elicitor activity in suspension-cultured cells of three dicot species.					
34364593	8	40	theme	elicitor-active	1008:1022	arg1	HexA-Rha					1030:1037	HexA-Rha	1030:1037	HexA-Rha	1030:1037	By identifying the smallest elicitor-active unit, HexA-Rha, we took an important next step to understand how the structure influences ulvan elicitor responses.					
34364593	8	40	theme	elicitor-active	1008:1022	arg1	unit					1024:1027	the smallest elicitor-active unit	995:1027	the smallest elicitor-active unit	995:1027	By identifying the smallest elicitor-active unit, HexA-Rha, we took an important next step to understand how the structure influences ulvan elicitor responses.					
34364593	2	41	theme	ulvan	323:327	arg1	polymers					329:336	ulvan polymers	323:336	ulvan polymers	323:336	We analyzed the structure-function relationship of ulvan polymers and oligomers for their elicitor activity in suspension-cultured cells of three dicot species.					
34364593	0	42	theme	active	63:68	arg1	unit					70:73	the smallest active unit	50:73	the smallest active unit able to induce an oxidative burst in dicot cells	50:122	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	0	42	theme	active	63:68	arg1	acid					32:35	The non-sulfated ulvanobiuronic acid	0:35	The non-sulfated ulvanobiuronic acid of ulvans	0:45	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	8	43	theme	smallest	999:1006	arg1	HexA-Rha					1030:1037	HexA-Rha	1030:1037	HexA-Rha	1030:1037	By identifying the smallest elicitor-active unit, HexA-Rha, we took an important next step to understand how the structure influences ulvan elicitor responses.					
34364593	8	43	theme	smallest	999:1006	arg1	unit					1024:1027	the smallest elicitor-active unit	995:1027	the smallest elicitor-active unit	995:1027	By identifying the smallest elicitor-active unit, HexA-Rha, we took an important next step to understand how the structure influences ulvan elicitor responses.					
34364593	3	44	theme	sulfation	541:549	arg1	composition					518:528	its monosaccharide composition	499:528	its monosaccharide composition	499:528	The polysaccharide from Ulva fasciata was characterized regarding its monosaccharide composition, degree of sulfation, and molecular mass.					
34364593	3	44	theme	sulfation	541:549	arg1	mass					566:569	molecular mass	556:569	molecular mass	556:569	The polysaccharide from Ulva fasciata was characterized regarding its monosaccharide composition, degree of sulfation, and molecular mass.					
34364593	3	44	theme	sulfation	541:549	arg1	degree					531:536	degree	531:536	degree of sulfation	531:549	The polysaccharide from Ulva fasciata was characterized regarding its monosaccharide composition, degree of sulfation, and molecular mass.					
34364593	3	45	theme	monosaccharide	503:516	arg1	composition					518:528	its monosaccharide composition	499:528	its monosaccharide composition	499:528	The polysaccharide from Ulva fasciata was characterized regarding its monosaccharide composition, degree of sulfation, and molecular mass.					
34364593	1	46	theme	environmentally	211:225	arg1	they					202:205	they	202:205	they	202:205	Ulvans from green algae are promising compounds for plant protection because they are environmentally friendly and induce plant defense responses.					
34364593	1	46	theme	environmentally	211:225	arg1	friendly					227:234	environmentally friendly	211:234	environmentally friendly	211:234	Ulvans from green algae are promising compounds for plant protection because they are environmentally friendly and induce plant defense responses.					
34364593	0	47	theme	smallest	54:61	arg1	unit					70:73	the smallest active unit	50:73	the smallest active unit able to induce an oxidative burst in dicot cells	50:122	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	0	47	theme	smallest	54:61	arg1	acid					32:35	The non-sulfated ulvanobiuronic acid	0:35	The non-sulfated ulvanobiuronic acid of ulvans	0:45	The non-sulfated ulvanobiuronic acid of ulvans is the smallest active unit able to induce an oxidative burst in dicot cells.					
34364593	1	48	from	algae	143:147	arg1	Ulvans					125:130	Ulvans	125:130	Ulvans from green algae	125:147	Ulvans from green algae are promising compounds for plant protection because they are environmentally friendly and induce plant defense responses.					
34364593	1	48	from	algae	143:147	arg1	compounds					163:171	promising compounds	153:171	promising compounds for plant protection	153:192	Ulvans from green algae are promising compounds for plant protection because they are environmentally friendly and induce plant defense responses.					
34364593	2	49	theme	elicitor	362:369	arg1	activity					371:378	their elicitor activity	356:378	their elicitor activity	356:378	We analyzed the structure-function relationship of ulvan polymers and oligomers for their elicitor activity in suspension-cultured cells of three dicot species.					
34364593	7	50	theme	ulvan	940:944	arg1	dimers					946:951	the ulvan dimers	936:951	the ulvan dimers	936:951	The elicitor activity of the ulvan dimers did not require sulfation.					
32658237	7	0	theme	correlation	1116:1126	arg1	analysis					1128:1135	The correlation analysis	1112:1135	The correlation analysis	1112:1135	The correlation analysis indicated that the protective effects of FCPS on ulcerative colitis might be highly correlated with the microbiota composition changes and the formation of SCFAs.					
32658237	1	1	theme	fig	151:153	arg1	fruit					155:159	the common fig fruit	140:159	the common fig fruit	140:159	The Ficus carica polysaccharide (FCPS) components of the common fig fruit have been demonstrated to exhibit antioxidant and immunity-enhancing activities.					
32658237	5	2	theme	FCPS	735:738	arg1	supplementation					740:754	FCPS supplementation	735:754	FCPS supplementation	735:754	FCPS supplementation significantly reformed the gut microbiome by enhancing the abundance of S24-7, Bacteroides, and Coprococus, and suppressing the abundance of Escherichia and Clostridium at the genus level.					
32658237	3	3	dep	FCPS	376:379	arg1	mg					390:391	150-300 mg	382:391	150-300 mg per kg bw	382:401	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	1	4	theme	fruit	155:159	arg1	components					126:135	The Ficus carica polysaccharide (FCPS) components	87:135	The Ficus carica polysaccharide (FCPS) components of the common fig fruit	87:159	The Ficus carica polysaccharide (FCPS) components of the common fig fruit have been demonstrated to exhibit antioxidant and immunity-enhancing activities.					
32658237	4	5	theme	FCPS	562:565	arg1	treatment					567:575	FCPS treatment	562:575	FCPS treatment	562:575	FCPS treatment protected the goblet cells, elevated the expression of tight junction protein claudin-1, and suppressed the formation of cytokines including TNF-α and IL-1β.					
32658237	3	6	theme	week	351:354	arg1	FCPS					376:379	orally administered FCPS	356:379	5 week orally administered FCPS (150-300 mg per kg bw)	349:402	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	3	7	theme	inflammatory	531:542	arg1	cells					544:548	inflammatory cells	531:548	inflammatory cells	531:548	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	8	8	theme	nutritional	1386:1396	arg1	supplementation					1349:1363	FCPS supplementation	1344:1363	FCPS supplementation	1344:1363	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	8	8	theme	nutritional	1386:1396	arg1	strategy					1398:1405	a promising nutritional strategy	1374:1405	a promising nutritional strategy for reducing inflammatory bowel disease	1374:1445	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	6	9	theme	microbial	987:995	arg1	metabolites					997:1007	beneficial microbial metabolites	976:1007	beneficial microbial metabolites	976:1007	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	9	theme	microbial	987:995	arg1	acids					1028:1032	short chain fatty acids	1010:1032	short chain fatty acids	1010:1032	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	5	10	theme	genus	932:936	arg1	level					938:942	the genus level	928:942	the genus level	928:942	FCPS supplementation significantly reformed the gut microbiome by enhancing the abundance of S24-7, Bacteroides, and Coprococus, and suppressing the abundance of Escherichia and Clostridium at the genus level.					
32658237	7	11	from	effects	1167:1173	arg1	colitis					1197:1203	ulcerative colitis	1186:1203	ulcerative colitis	1186:1203	The correlation analysis indicated that the protective effects of FCPS on ulcerative colitis might be highly correlated with the microbiota composition changes and the formation of SCFAs.					
32658237	6	12	theme	beneficial	976:985	arg1	metabolites					997:1007	beneficial microbial metabolites	976:1007	beneficial microbial metabolites	976:1007	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	12	theme	beneficial	976:985	arg1	acids					1028:1032	short chain fatty acids	1010:1032	short chain fatty acids	1010:1032	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	7	13	theme	composition	1252:1262	arg1	changes					1264:1270	the microbiota composition changes	1237:1270	the microbiota composition changes	1237:1270	The correlation analysis indicated that the protective effects of FCPS on ulcerative colitis might be highly correlated with the microbiota composition changes and the formation of SCFAs.					
32658237	3	14	theme	cells	544:548	arg1	infiltration					515:526	the infiltration	511:526	the infiltration of inflammatory cells in the gut	511:559	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	5	15	theme	Coprococus	852:861	arg1	abundance					815:823	the abundance	811:823	the abundance of S24-7, Bacteroides, and Coprococus	811:861	FCPS supplementation significantly reformed the gut microbiome by enhancing the abundance of S24-7, Bacteroides, and Coprococus, and suppressing the abundance of Escherichia and Clostridium at the genus level.					
32658237	7	16	theme	ulcerative	1186:1195	arg1	colitis					1197:1203	ulcerative colitis	1186:1203	ulcerative colitis	1186:1203	The correlation analysis indicated that the protective effects of FCPS on ulcerative colitis might be highly correlated with the microbiota composition changes and the formation of SCFAs.					
32658237	0	17	from	colitis	62:68	arg1	mice					81:84	C57BL/6 mice	73:84	C57BL/6 mice	73:84	Ficus carica polysaccharide attenuates DSS-induced ulcerative colitis in C57BL/6 mice.					
32658237	6	18	theme	FCPS-treated	1085:1096	arg1	mice					1106:1109	FCPS-treated colitis mice	1085:1109	FCPS-treated colitis mice	1085:1109	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	5	19	theme	Bacteroides	835:845	arg1	abundance					815:823	the abundance	811:823	the abundance of S24-7, Bacteroides, and Coprococus	811:861	FCPS supplementation significantly reformed the gut microbiome by enhancing the abundance of S24-7, Bacteroides, and Coprococus, and suppressing the abundance of Escherichia and Clostridium at the genus level.					
32658237	4	20	theme	protein	647:653	arg1	claudin-1					655:663	tight junction protein claudin-1	632:663	tight junction protein claudin-1	632:663	FCPS treatment protected the goblet cells, elevated the expression of tight junction protein claudin-1, and suppressed the formation of cytokines including TNF-α and IL-1β.					
32658237	3	21	theme	5	349:349	arg1	week					351:354	week	351:354	week	351:354	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	5	22	theme	Escherichia	897:907	arg1	abundance					884:892	the abundance	880:892	the abundance of Escherichia and Clostridium	880:923	FCPS supplementation significantly reformed the gut microbiome by enhancing the abundance of S24-7, Bacteroides, and Coprococus, and suppressing the abundance of Escherichia and Clostridium at the genus level.					
32658237	5	23	theme	gut	783:785	arg1	microbiome					787:796	the gut microbiome	779:796	the gut microbiome	779:796	FCPS supplementation significantly reformed the gut microbiome by enhancing the abundance of S24-7, Bacteroides, and Coprococus, and suppressing the abundance of Escherichia and Clostridium at the genus level.					
32658237	6	24	theme	fatty	1022:1026	arg1	acetate					1046:1052	acetate	1046:1052	acetate	1046:1052	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	24	theme	fatty	1022:1026	arg1	metabolites					997:1007	beneficial microbial metabolites	976:1007	beneficial microbial metabolites	976:1007	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	24	theme	fatty	1022:1026	arg1	butyrate					1058:1065	butyrate	1058:1065	butyrate	1058:1065	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	24	theme	fatty	1022:1026	arg1	acids					1028:1032	short chain fatty acids	1010:1032	short chain fatty acids	1010:1032	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	3	25	theme	DSS-induced	428:438	arg1	colitis					440:446	DSS-induced colitis	428:446	DSS-induced colitis in C57BL/6J mice	428:463	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	0	26	theme	carica	6:11	arg1	polysaccharide					13:26	Ficus carica polysaccharide	0:26	Ficus carica polysaccharide	0:26	Ficus carica polysaccharide attenuates DSS-induced ulcerative colitis in C57BL/6 mice.					
32658237	1	27	theme	Ficus	91:95	arg1	components					126:135	The Ficus carica polysaccharide (FCPS) components	87:135	The Ficus carica polysaccharide (FCPS) components of the common fig fruit	87:159	The Ficus carica polysaccharide (FCPS) components of the common fig fruit have been demonstrated to exhibit antioxidant and immunity-enhancing activities.					
32658237	7	28	theme	FCPS	1178:1181	arg1	effects					1167:1173	the protective effects	1152:1173	the protective effects of FCPS on ulcerative colitis	1152:1203	The correlation analysis indicated that the protective effects of FCPS on ulcerative colitis might be highly correlated with the microbiota composition changes and the formation of SCFAs.					
32658237	0	29	theme	Ficus	0:4	arg1	polysaccharide					13:26	Ficus carica polysaccharide	0:26	Ficus carica polysaccharide	0:26	Ficus carica polysaccharide attenuates DSS-induced ulcerative colitis in C57BL/6 mice.					
32658237	7	30	theme	microbiota	1241:1250	arg1	changes					1264:1270	the microbiota composition changes	1237:1270	the microbiota composition changes	1237:1270	The correlation analysis indicated that the protective effects of FCPS on ulcerative colitis might be highly correlated with the microbiota composition changes and the formation of SCFAs.					
32658237	1	31	theme	carica	97:102	arg1	components					126:135	The Ficus carica polysaccharide (FCPS) components	87:135	The Ficus carica polysaccharide (FCPS) components of the common fig fruit	87:159	The Ficus carica polysaccharide (FCPS) components of the common fig fruit have been demonstrated to exhibit antioxidant and immunity-enhancing activities.					
32658237	4	32	theme	claudin-1	655:663	arg1	expression					618:627	the expression	614:627	the expression of tight junction protein claudin-1	614:663	FCPS treatment protected the goblet cells, elevated the expression of tight junction protein claudin-1, and suppressed the formation of cytokines including TNF-α and IL-1β.					
32658237	3	33	theme	administered	363:374	arg1	FCPS					376:379	orally administered FCPS	356:379	5 week orally administered FCPS (150-300 mg per kg bw)	349:402	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	2	34	theme	colitis	305:311	arg1	development					313:323	the ulcerative colitis development	290:323	the ulcerative colitis development	290:323	However, it is unclear whether it could prevent the ulcerative colitis development.					
32658237	3	35	theme	C57BL/6J	451:458	arg1	mice					460:463	C57BL/6J mice	451:463	C57BL/6J mice	451:463	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	5	36	theme	S24-7	828:832	arg1	abundance					815:823	the abundance	811:823	the abundance of S24-7, Bacteroides, and Coprococus	811:861	FCPS supplementation significantly reformed the gut microbiome by enhancing the abundance of S24-7, Bacteroides, and Coprococus, and suppressing the abundance of Escherichia and Clostridium at the genus level.					
32658237	2	37	theme	ulcerative	294:303	arg1	development					313:323	the ulcerative colitis development	290:323	the ulcerative colitis development	290:323	However, it is unclear whether it could prevent the ulcerative colitis development.					
32658237	7	38	theme	protective	1156:1165	arg1	effects					1167:1173	the protective effects	1152:1173	the protective effects of FCPS on ulcerative colitis	1152:1203	The correlation analysis indicated that the protective effects of FCPS on ulcerative colitis might be highly correlated with the microbiota composition changes and the formation of SCFAs.					
32658237	1	39	theme	antioxidant	195:205	arg1	activities					230:239	antioxidant and immunity-enhancing activities	195:239	antioxidant and immunity-enhancing activities	195:239	The Ficus carica polysaccharide (FCPS) components of the common fig fruit have been demonstrated to exhibit antioxidant and immunity-enhancing activities.					
32658237	0	40	theme	ulcerative	51:60	arg1	colitis					62:68	DSS-induced ulcerative colitis	39:68	DSS-induced ulcerative colitis in C57BL/6 mice	39:84	Ficus carica polysaccharide attenuates DSS-induced ulcerative colitis in C57BL/6 mice.					
32658237	5	41	theme	Clostridium	913:923	arg1	abundance					884:892	the abundance	880:892	the abundance of Escherichia and Clostridium	880:923	FCPS supplementation significantly reformed the gut microbiome by enhancing the abundance of S24-7, Bacteroides, and Coprococus, and suppressing the abundance of Escherichia and Clostridium at the genus level.					
32658237	4	42	theme	tight	632:636	arg1	claudin-1					655:663	tight junction protein claudin-1	632:663	tight junction protein claudin-1	632:663	FCPS treatment protected the goblet cells, elevated the expression of tight junction protein claudin-1, and suppressed the formation of cytokines including TNF-α and IL-1β.					
32658237	0	43	theme	DSS-induced	39:49	arg1	colitis					62:68	DSS-induced ulcerative colitis	39:68	DSS-induced ulcerative colitis in C57BL/6 mice	39:84	Ficus carica polysaccharide attenuates DSS-induced ulcerative colitis in C57BL/6 mice.					
32658237	6	44	theme	metabolites	997:1007	arg1	formation					963:971	the formation	959:971	the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate,	959:1066	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	3	45	from	colitis	440:446	arg1	mice					460:463	C57BL/6J mice	451:463	C57BL/6J mice	451:463	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	1	46	theme	immunity-enhancing	211:228	arg1	activities					230:239	antioxidant and immunity-enhancing activities	195:239	antioxidant and immunity-enhancing activities	195:239	The Ficus carica polysaccharide (FCPS) components of the common fig fruit have been demonstrated to exhibit antioxidant and immunity-enhancing activities.					
32658237	8	47	theme	promising	1376:1384	arg1	supplementation					1349:1363	FCPS supplementation	1344:1363	FCPS supplementation	1344:1363	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	8	47	theme	promising	1376:1384	arg1	strategy					1398:1405	a promising nutritional strategy	1374:1405	a promising nutritional strategy for reducing inflammatory bowel disease	1374:1445	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	8	48	theme	essential	1473:1481	arg1	roles					1483:1487	essential roles	1473:1487	essential roles	1473:1487	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	8	49	theme	bowel	1433:1437	arg1	disease					1439:1445	inflammatory bowel disease	1420:1445	inflammatory bowel disease	1420:1445	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	1	50	theme	FCPS	120:123	arg1	components					126:135	The Ficus carica polysaccharide (FCPS) components	87:135	The Ficus carica polysaccharide (FCPS) components of the common fig fruit	87:159	The Ficus carica polysaccharide (FCPS) components of the common fig fruit have been demonstrated to exhibit antioxidant and immunity-enhancing activities.					
32658237	8	51	theme	inflammatory	1420:1431	arg1	disease					1439:1445	inflammatory bowel disease	1420:1445	inflammatory bowel disease	1420:1445	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	3	52	theme	colon	482:486	arg1	length					488:493	the colon length	478:493	the colon length	478:493	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	4	53	theme	cytokines	698:706	arg1	formation					685:693	the formation	681:693	the formation of cytokines including TNF-α and IL-1β	681:732	FCPS treatment protected the goblet cells, elevated the expression of tight junction protein claudin-1, and suppressed the formation of cytokines including TNF-α and IL-1β.					
32658237	1	54	theme	polysaccharide	104:117	arg1	components					126:135	The Ficus carica polysaccharide (FCPS) components	87:135	The Ficus carica polysaccharide (FCPS) components of the common fig fruit	87:159	The Ficus carica polysaccharide (FCPS) components of the common fig fruit have been demonstrated to exhibit antioxidant and immunity-enhancing activities.					
32658237	8	55	theme	beneficial	1508:1517	arg1	effects					1519:1525	these beneficial effects	1502:1525	these beneficial effects	1502:1525	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	6	56	theme	colitis	1098:1104	arg1	mice					1106:1109	FCPS-treated colitis mice	1085:1109	FCPS-treated colitis mice	1085:1109	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	8	57	theme	FCPS	1344:1347	arg1	supplementation					1349:1363	FCPS supplementation	1344:1363	FCPS supplementation	1344:1363	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	8	57	theme	FCPS	1344:1347	arg1	strategy					1398:1405	a promising nutritional strategy	1374:1405	a promising nutritional strategy for reducing inflammatory bowel disease	1374:1445	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	4	58	theme	goblet	591:596	arg1	cells					598:602	the goblet cells	587:602	the goblet cells	587:602	FCPS treatment protected the goblet cells, elevated the expression of tight junction protein claudin-1, and suppressed the formation of cytokines including TNF-α and IL-1β.					
32658237	8	59	theme	gut	1455:1457	arg1	microbes					1459:1466	the gut microbes	1451:1466	the gut microbes	1451:1466	In conclusion, these results indicated that FCPS supplementation could be a promising nutritional strategy for reducing inflammatory bowel disease and the gut microbes play essential roles in providing these beneficial effects.					
32658237	4	60	theme	junction	638:645	arg1	claudin-1					655:663	tight junction protein claudin-1	632:663	tight junction protein claudin-1	632:663	FCPS treatment protected the goblet cells, elevated the expression of tight junction protein claudin-1, and suppressed the formation of cytokines including TNF-α and IL-1β.					
32658237	6	61	theme	chain	1016:1020	arg1	acetate					1046:1052	acetate	1046:1052	acetate	1046:1052	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	61	theme	chain	1016:1020	arg1	metabolites					997:1007	beneficial microbial metabolites	976:1007	beneficial microbial metabolites	976:1007	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	61	theme	chain	1016:1020	arg1	butyrate					1058:1065	butyrate	1058:1065	butyrate	1058:1065	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	61	theme	chain	1016:1020	arg1	acids					1028:1032	short chain fatty acids	1010:1032	short chain fatty acids	1010:1032	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	0	62	theme	C57BL/6	73:79	arg1	mice					81:84	C57BL/6 mice	73:84	C57BL/6 mice	73:84	Ficus carica polysaccharide attenuates DSS-induced ulcerative colitis in C57BL/6 mice.					
32658237	7	63	theme	SCFAs	1293:1297	arg1	changes					1264:1270	the microbiota composition changes	1237:1270	the microbiota composition changes	1237:1270	The correlation analysis indicated that the protective effects of FCPS on ulcerative colitis might be highly correlated with the microbiota composition changes and the formation of SCFAs.					
32658237	7	63	theme	SCFAs	1293:1297	arg1	formation					1280:1288	the formation	1276:1288	the formation of SCFAs	1276:1297	The correlation analysis indicated that the protective effects of FCPS on ulcerative colitis might be highly correlated with the microbiota composition changes and the formation of SCFAs.					
32658237	1	64	theme	common	144:149	arg1	fruit					155:159	the common fig fruit	140:159	the common fig fruit	140:159	The Ficus carica polysaccharide (FCPS) components of the common fig fruit have been demonstrated to exhibit antioxidant and immunity-enhancing activities.					
32658237	3	65	from	infiltration	515:526	arg1	gut					557:559	the gut	553:559	the gut	553:559	Here, we reported that 5 week orally administered FCPS (150-300 mg per kg bw) significantly prevented DSS-induced colitis in C57BL/6J mice by improving the colon length and suppressing the infiltration of inflammatory cells in the gut.					
32658237	6	66	theme	short	1010:1014	arg1	acetate					1046:1052	acetate	1046:1052	acetate	1046:1052	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	66	theme	short	1010:1014	arg1	metabolites					997:1007	beneficial microbial metabolites	976:1007	beneficial microbial metabolites	976:1007	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	66	theme	short	1010:1014	arg1	butyrate					1058:1065	butyrate	1058:1065	butyrate	1058:1065	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
32658237	6	66	theme	short	1010:1014	arg1	acids					1028:1032	short chain fatty acids	1010:1032	short chain fatty acids	1010:1032	Consistently, the formation of beneficial microbial metabolites, short chain fatty acids, especially acetate and butyrate, were improved in FCPS-treated colitis mice.					
33207378	2	0	from	disintegration	433:446	arg1	buffer					461:466	phosphate buffer	451:466	phosphate buffer	451:466	Physicochemical properties, such as moisture, mucoadhesion, thickness, tensile strength, disintegration in phosphate buffer were determined in obtained samples of this dosage form.					
33207378	1	1	with	films	148:152	arg1	trometamine					169:179	ketorolac trometamine	159:179	ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol	159:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	0	2	with	Technologies	31:42	arg1	Trometamine					74:84	Ketorolac Trometamine	64:84	Ketorolac Trometamine	64:84	Development of Composition and Technologies of Dental Film with Ketorolac Trometamine.					
33207378	1	3	theme	films	148:152	arg1	development					126:136	the development	122:136	the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol	122:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	1	4	theme	sodium	244:249	arg1	alginates					251:259	sodium alginates	244:259	sodium alginates	244:259	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	3	5	theme	ketorolac	713:721	arg1	trometamine					723:733	ketorolac trometamine	713:733	ketorolac trometamine for use in dentistry	713:754	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	1	6	theme	alginates	251:259	arg1	groups					234:239	the groups	230:239	the groups of sodium alginates and xanthan gums	230:276	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	1	6	theme	alginates	251:259	arg1	alginates					251:259	sodium alginates	244:259	sodium alginates	244:259	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	1	6	theme	alginates	251:259	arg1	gums					273:276	xanthan gums	265:276	xanthan gums	265:276	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	3	7	theme	properties	557:566	arg1	model					607:611	commercial model	596:611	commercial model of dental film	596:626	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	3	7	theme	properties	557:566	arg1	comparison					527:536	A comparison	525:536	A comparison of physicochemical properties of experimental samples	525:590	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	1	8	from	polymers	216:223	arg1	combination					281:291	combination	281:291	combination with lightly crosslinked acrylic polymer carbopol	281:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	1	9	theme	xanthan	265:271	arg1	gums					273:276	xanthan gums	265:276	xanthan gums	265:276	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	1	10	theme	ketorolac	159:167	arg1	trometamine					169:179	ketorolac trometamine	159:179	ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol	159:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	2	11	theme	form	519:522	arg1	samples					496:502	obtained samples	487:502	obtained samples of this dosage form	487:522	Physicochemical properties, such as moisture, mucoadhesion, thickness, tensile strength, disintegration in phosphate buffer were determined in obtained samples of this dosage form.					
33207378	1	12	with	combination	281:291	arg1	carbopol					334:341	lightly crosslinked acrylic polymer carbopol	298:341	lightly crosslinked acrylic polymer carbopol	298:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	3	13	theme	physicochemical	541:555	arg1	properties					557:566	physicochemical properties	541:566	physicochemical properties of experimental samples	541:590	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	3	14	with	films	702:706	arg1	trometamine					723:733	ketorolac trometamine	713:733	ketorolac trometamine for use in dentistry	713:754	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	2	15	theme	dosage	512:517	arg1	form					519:522	this dosage form	507:522	this dosage form	507:522	Physicochemical properties, such as moisture, mucoadhesion, thickness, tensile strength, disintegration in phosphate buffer were determined in obtained samples of this dosage form.					
33207378	2	16	theme	phosphate	451:459	arg1	buffer					461:466	phosphate buffer	451:466	phosphate buffer	451:466	Physicochemical properties, such as moisture, mucoadhesion, thickness, tensile strength, disintegration in phosphate buffer were determined in obtained samples of this dosage form.					
33207378	1	17	theme	gums	273:276	arg1	groups					234:239	the groups	230:239	the groups of sodium alginates and xanthan gums	230:276	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	1	17	theme	gums	273:276	arg1	alginates					251:259	sodium alginates	244:259	sodium alginates	244:259	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	1	17	theme	gums	273:276	arg1	gums					273:276	xanthan gums	265:276	xanthan gums	265:276	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	3	18	theme	commercial	596:605	arg1	model					607:611	commercial model	596:611	commercial model of dental film	596:626	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	3	19	theme	complex	684:690	arg1	matrix					692:697	complex matrix	684:697	complex matrix of films with ketorolac trometamine for use in dentistry	684:754	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	3	20	theme	experimental	571:582	arg1	samples					584:590	experimental samples	571:590	experimental samples	571:590	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	0	21	theme	Composition	15:25	arg1	Development					0:10	Development	0:10	Development of Composition and Technologies of Dental Film with Ketorolac Trometamine.	0:85	Development of Composition and Technologies of Dental Film with Ketorolac Trometamine.					
33207378	3	22	theme	samples	584:590	arg1	properties					557:566	physicochemical properties	541:566	physicochemical properties of experimental samples	541:590	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	1	23	theme	natural	194:200	arg1	polymers					216:223	the natural biodegradable polymers	190:223	the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol	190:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	2	24	theme	obtained	487:494	arg1	samples					496:502	obtained samples	487:502	obtained samples of this dosage form	487:522	Physicochemical properties, such as moisture, mucoadhesion, thickness, tensile strength, disintegration in phosphate buffer were determined in obtained samples of this dosage form.					
33207378	2	25	theme	tensile	415:421	arg1	strength					423:430	tensile strength	415:430	tensile strength	415:430	Physicochemical properties, such as moisture, mucoadhesion, thickness, tensile strength, disintegration in phosphate buffer were determined in obtained samples of this dosage form.					
33207378	2	25	theme	tensile	415:421	arg1	moisture					380:387	moisture	380:387	moisture	380:387	Physicochemical properties, such as moisture, mucoadhesion, thickness, tensile strength, disintegration in phosphate buffer were determined in obtained samples of this dosage form.					
33207378	3	26	theme	dental	616:621	arg1	film					623:626	dental film	616:626	dental film	616:626	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	1	27	theme	biodegradable	202:214	arg1	polymers					216:223	the natural biodegradable polymers	190:223	the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol	190:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	0	28	theme	Technologies	31:42	arg1	Development					0:10	Development	0:10	Development of Composition and Technologies of Dental Film with Ketorolac Trometamine.	0:85	Development of Composition and Technologies of Dental Film with Ketorolac Trometamine.					
33207378	3	29	theme	perspective	657:667	arg1	composition					669:679	the perspective composition	653:679	the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry	653:754	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	3	30	theme	film	623:626	arg1	model					607:611	commercial model	596:611	commercial model of dental film	596:626	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	3	30	theme	film	623:626	arg1	comparison					527:536	A comparison	525:536	A comparison of physicochemical properties of experimental samples	525:590	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	0	31	theme	Film	54:57	arg1	Composition					15:25	Composition	15:25	Composition	15:25	Development of Composition and Technologies of Dental Film with Ketorolac Trometamine.					
33207378	0	31	theme	Film	54:57	arg1	Technologies					31:42	Technologies	31:42	Technologies of Dental Film with Ketorolac Trometamine	31:84	Development of Composition and Technologies of Dental Film with Ketorolac Trometamine.					
33207378	1	32	theme	crosslinked	306:316	arg1	carbopol					334:341	lightly crosslinked acrylic polymer carbopol	298:341	lightly crosslinked acrylic polymer carbopol	298:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	1	33	from	groups	234:239	arg1	polymers					216:223	the natural biodegradable polymers	190:223	the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol	190:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	0	34	with	Composition	15:25	arg1	Trometamine					74:84	Ketorolac Trometamine	64:84	Ketorolac Trometamine	64:84	Development of Composition and Technologies of Dental Film with Ketorolac Trometamine.					
33207378	0	35	theme	Dental	47:52	arg1	Film					54:57	Dental Film	47:57	Dental Film	47:57	Development of Composition and Technologies of Dental Film with Ketorolac Trometamine.					
33207378	1	36	theme	acrylic	318:324	arg1	carbopol					334:341	lightly crosslinked acrylic polymer carbopol	298:341	lightly crosslinked acrylic polymer carbopol	298:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	3	37	theme	films	702:706	arg1	matrix					692:697	complex matrix	684:697	complex matrix of films with ketorolac trometamine for use in dentistry	684:754	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	2	38	theme	Physicochemical	344:358	arg1	properties					360:369	Physicochemical properties	344:369	Physicochemical properties	344:369	Physicochemical properties, such as moisture, mucoadhesion, thickness, tensile strength, disintegration in phosphate buffer were determined in obtained samples of this dosage form.					
33207378	2	38	theme	Physicochemical	344:358	arg1	moisture					380:387	moisture	380:387	moisture	380:387	Physicochemical properties, such as moisture, mucoadhesion, thickness, tensile strength, disintegration in phosphate buffer were determined in obtained samples of this dosage form.					
33207378	1	39	theme	polymer	326:332	arg1	carbopol					334:341	lightly crosslinked acrylic polymer carbopol	298:341	lightly crosslinked acrylic polymer carbopol	298:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	1	40	link	crosslinked	306:316	arg1	carbopol					334:341	lightly crosslinked acrylic polymer carbopol	298:341	lightly crosslinked acrylic polymer carbopol	298:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	3	41	from	use	739:741	arg1	dentistry					746:754	dentistry	746:754	dentistry	746:754	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	3	42	theme	matrix	692:697	arg1	composition					669:679	the perspective composition	653:679	the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry	653:754	A comparison of physicochemical properties of experimental samples and commercial model of dental film has allowed establishing the perspective composition of complex matrix of films with ketorolac trometamine for use in dentistry.					
33207378	1	43	theme	development	126:136	arg1	results					111:117	the results	107:117	the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol	107:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
33207378	0	44	theme	Ketorolac	64:72	arg1	Trometamine					74:84	Ketorolac Trometamine	64:84	Ketorolac Trometamine	64:84	Development of Composition and Technologies of Dental Film with Ketorolac Trometamine.					
33207378	1	45	theme	dental	141:146	arg1	films					148:152	dental films	141:152	dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol	141:341	The report presents the results of the development of dental films with ketorolac trometamine based on the natural biodegradable polymers from the groups of sodium alginates and xanthan gums in combination with lightly crosslinked acrylic polymer carbopol.					
32585275	0	0	theme	essential	103:111	arg1	oil					113:115	peppermint essential oil	92:115	peppermint essential oil	92:115	Alginate/maltodextrin and alginate/shellac gum core-shell capsules for the encapsulation of peppermint essential oil.					
32585275	5	1	theme	secondary	878:886	arg1	excipient					888:896	secondary excipient	878:896	secondary excipient	878:896	Neither the type nor the amount of secondary excipient influenced wet capsule particle size (about 4 mm).					
32585275	8	2	dep	the	1481:1483	arg1	needs					1485:1489	needs	1485:1489	needs	1485:1489	In addition, the amount of maltodextrin included in the formulations affected the swelling behavior of the dried capsules in water and this leads considering maltodextrin a useful excipient to modulate swelling according to the needs of the different applications.					
32585275	0	3	theme	peppermint	92:101	arg1	oil					113:115	peppermint essential oil	92:115	peppermint essential oil	92:115	Alginate/maltodextrin and alginate/shellac gum core-shell capsules for the encapsulation of peppermint essential oil.					
32585275	6	4	theme	particle	963:970	arg1	size					972:975	particle size	963:975	particle size	963:975	After drying, particle size decreased (it was about 2 mm) and while shellac gum amount did not affect dried capsule dimensions, an increase in maltodextrin amount corresponded to an increase in their diameters.					
32585275	5	5	theme	excipient	888:896	arg1	amount					868:873	the amount	864:873	the amount of secondary excipient	864:896	Neither the type nor the amount of secondary excipient influenced wet capsule particle size (about 4 mm).					
32585275	5	5	theme	excipient	888:896	arg1	excipient					888:896	secondary excipient	878:896	secondary excipient	878:896	Neither the type nor the amount of secondary excipient influenced wet capsule particle size (about 4 mm).					
32585275	4	6	theme	qualitative/quantitative	580:603	arg1	modifications					605:617	The qualitative/quantitative modifications	576:617	The qualitative/quantitative modifications of capsule shell composition	576:646	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	4	7	dep	content	690:696	arg1	%					718:718	more than 50% w/w	706:722	more than 50% w/w	706:722	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	7	8	from	amount	1206:1211	arg1	formulations					1243:1254	the formulations	1239:1254	the formulations	1239:1254	Capsule hardness diminished by increasing the amount of secondary excipient in the formulations.					
32585275	2	9	theme	ionotropic	322:331	arg1	gelation					333:340	inverse ionotropic gelation	314:340	inverse ionotropic gelation	314:340	Core-shell systems containing peppermint essential oil have been obtained by inverse ionotropic gelation.					
32585275	8	10	theme	swelling	1339:1346	arg1	behavior					1348:1355	the swelling behavior	1335:1355	the swelling behavior of the dried capsules in water	1335:1386	In addition, the amount of maltodextrin included in the formulations affected the swelling behavior of the dried capsules in water and this leads considering maltodextrin a useful excipient to modulate swelling according to the needs of the different applications.					
32585275	4	11	theme	capsule	622:628	arg1	composition					636:646	capsule shell composition	622:646	capsule shell composition	622:646	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	0	12	theme	oil	113:115	arg1	encapsulation					75:87	the encapsulation	71:87	the encapsulation of peppermint essential oil	71:115	Alginate/maltodextrin and alginate/shellac gum core-shell capsules for the encapsulation of peppermint essential oil.					
32585275	4	13	theme	other	746:750	arg1	properties					766:775	other dried capsule properties	746:775	other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior	746:840	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	2	14	theme	inverse	314:320	arg1	gelation					333:340	inverse ionotropic gelation	314:340	inverse ionotropic gelation	314:340	Core-shell systems containing peppermint essential oil have been obtained by inverse ionotropic gelation.					
32585275	5	15	theme	particle	921:928	arg1	size					930:933	wet capsule particle size	909:933	wet capsule particle size (about 4 mm)	909:946	Neither the type nor the amount of secondary excipient influenced wet capsule particle size (about 4 mm).					
32585275	4	16	theme	limited	654:660	arg1	impact					662:667	a limited impact	652:667	a limited impact	652:667	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	3	17	theme	shellac	540:546	arg1	gum					548:550	shellac gum	540:550	shellac gum	540:550	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W) was dripped in an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations.					
32585275	4	18	theme	oil	686:688	arg1	content					690:696	the essential oil content	672:696	the essential oil content (it was more than 50% w/w)	672:723	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	8	19	theme	different	1498:1506	arg1	applications					1508:1519	the different applications	1494:1519	the different applications	1494:1519	In addition, the amount of maltodextrin included in the formulations affected the swelling behavior of the dried capsules in water and this leads considering maltodextrin a useful excipient to modulate swelling according to the needs of the different applications.					
32585275	4	20	theme	swelling	824:831	arg1	behavior					833:840	swelling behavior	824:840	swelling behavior	824:840	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	4	21	theme	capsule	758:764	arg1	properties					766:775	other dried capsule properties	746:775	other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior	746:840	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	4	22	theme	essential	676:684	arg1	content					690:696	the essential oil content	672:696	the essential oil content (it was more than 50% w/w)	672:723	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	3	23	theme	CaCl2	401:405	arg1	solution					407:414	aqueous CaCl2 solution	393:414	aqueous CaCl2 solution enriched with hydroxyethylcellulose (W)	393:454	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W) was dripped in an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations.					
32585275	3	24	dep	excipient	513:521	arg1	maltodextrin					524:535	maltodextrin	524:535	maltodextrin	524:535	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W) was dripped in an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations.					
32585275	3	24	dep	excipient	513:521	arg1	gum					548:550	shellac gum	540:550	shellac gum	540:550	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W) was dripped in an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations.					
32585275	6	25	from	increase	1131:1138	arg1	diameters					1149:1157	their diameters	1143:1157	their diameters	1143:1157	After drying, particle size decreased (it was about 2 mm) and while shellac gum amount did not affect dried capsule dimensions, an increase in maltodextrin amount corresponded to an increase in their diameters.					
32585275	6	26	theme	gum	1025:1027	arg1	amount					1029:1034	shellac gum amount	1017:1034	shellac gum amount	1017:1034	After drying, particle size decreased (it was about 2 mm) and while shellac gum amount did not affect dried capsule dimensions, an increase in maltodextrin amount corresponded to an increase in their diameters.					
32585275	6	27	theme	shellac	1017:1023	arg1	amount					1029:1034	shellac gum amount	1017:1034	shellac gum amount	1017:1034	After drying, particle size decreased (it was about 2 mm) and while shellac gum amount did not affect dried capsule dimensions, an increase in maltodextrin amount corresponded to an increase in their diameters.					
32585275	8	28	theme	dried	1364:1368	arg1	capsules					1370:1377	the dried capsules	1360:1377	the dried capsules	1360:1377	In addition, the amount of maltodextrin included in the formulations affected the swelling behavior of the dried capsules in water and this leads considering maltodextrin a useful excipient to modulate swelling according to the needs of the different applications.					
32585275	6	29	theme	maltodextrin	1092:1103	arg1	amount					1105:1110	maltodextrin amount	1092:1110	maltodextrin amount	1092:1110	After drying, particle size decreased (it was about 2 mm) and while shellac gum amount did not affect dried capsule dimensions, an increase in maltodextrin amount corresponded to an increase in their diameters.					
32585275	3	30	theme	alginate	474:481	arg1	solution					483:490	an alginate solution	471:490	an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations	471:573	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W) was dripped in an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations.					
32585275	3	31	theme	aqueous	393:399	arg1	solution					407:414	aqueous CaCl2 solution	393:414	aqueous CaCl2 solution enriched with hydroxyethylcellulose (W)	393:454	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W) was dripped in an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations.					
32585275	1	32	dep	strategy	168:175	arg1	transform					195:203	transform	195:203	to transform them in free-flowing particles	192:234	Encapsulation of essential oils represents a good strategy to protect and to transform them in free-flowing particles.					
32585275	1	32	dep	strategy	168:175	arg1	protect					180:186	protect	180:186	to protect	177:186	Encapsulation of essential oils represents a good strategy to protect and to transform them in free-flowing particles.					
32585275	1	33	theme	free-flowing	213:224	arg1	particles					226:234	free-flowing particles	213:234	free-flowing particles	213:234	Encapsulation of essential oils represents a good strategy to protect and to transform them in free-flowing particles.					
32585275	3	34	theme	secondary	503:511	arg1	excipient					513:521	a secondary excipient	501:521	a secondary excipient (maltodextrin or shellac gum)	501:551	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W) was dripped in an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations.					
32585275	2	35	theme	peppermint	267:276	arg1	oil					288:290	peppermint essential oil	267:290	peppermint essential oil	267:290	Core-shell systems containing peppermint essential oil have been obtained by inverse ionotropic gelation.					
32585275	5	36	dep	size	930:933	arg1	4 mm					942:945	4 mm	942:945	4 mm	942:945	Neither the type nor the amount of secondary excipient influenced wet capsule particle size (about 4 mm).					
32585275	0	37	theme	alginate/shellac	26:41	arg1	gum					43:45	alginate/shellac gum	26:45	alginate/shellac gum	26:45	Alginate/maltodextrin and alginate/shellac gum core-shell capsules for the encapsulation of peppermint essential oil.					
32585275	5	38	theme	wet	909:911	arg1	size					930:933	wet capsule particle size	909:933	wet capsule particle size (about 4 mm)	909:946	Neither the type nor the amount of secondary excipient influenced wet capsule particle size (about 4 mm).					
32585275	8	39	theme	capsules	1370:1377	arg1	behavior					1348:1355	the swelling behavior	1335:1355	the swelling behavior of the dried capsules in water	1335:1386	In addition, the amount of maltodextrin included in the formulations affected the swelling behavior of the dried capsules in water and this leads considering maltodextrin a useful excipient to modulate swelling according to the needs of the different applications.					
32585275	6	40	theme	capsule	1057:1063	arg1	dimensions					1065:1074	dried capsule dimensions	1051:1074	dried capsule dimensions	1051:1074	After drying, particle size decreased (it was about 2 mm) and while shellac gum amount did not affect dried capsule dimensions, an increase in maltodextrin amount corresponded to an increase in their diameters.					
32585275	1	41	theme	essential	135:143	arg1	oils					145:148	essential oils	135:148	essential oils	135:148	Encapsulation of essential oils represents a good strategy to protect and to transform them in free-flowing particles.					
32585275	3	42	theme	O/W	346:348	arg1	emulsion					350:357	An O/W emulsion	343:357	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W)	343:454	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W) was dripped in an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations.					
32585275	2	43	theme	Core-shell	237:246	arg1	systems					248:254	Core-shell systems	237:254	Core-shell systems containing peppermint essential oil	237:290	Core-shell systems containing peppermint essential oil have been obtained by inverse ionotropic gelation.					
32585275	6	44	from	increase	1080:1087	arg1	amount					1105:1110	maltodextrin amount	1092:1110	maltodextrin amount	1092:1110	After drying, particle size decreased (it was about 2 mm) and while shellac gum amount did not affect dried capsule dimensions, an increase in maltodextrin amount corresponded to an increase in their diameters.					
32585275	1	45	theme	oils	145:148	arg1	Encapsulation					118:130	Encapsulation	118:130	Encapsulation of essential oils	118:148	Encapsulation of essential oils represents a good strategy to protect and to transform them in free-flowing particles.					
32585275	7	46	theme	secondary	1216:1224	arg1	excipient					1226:1234	secondary excipient	1216:1234	secondary excipient	1216:1234	Capsule hardness diminished by increasing the amount of secondary excipient in the formulations.					
32585275	8	47	theme	useful	1430:1435	arg1	excipient					1437:1445	a useful excipient	1428:1445	a useful excipient	1428:1445	In addition, the amount of maltodextrin included in the formulations affected the swelling behavior of the dried capsules in water and this leads considering maltodextrin a useful excipient to modulate swelling according to the needs of the different applications.					
32585275	4	48	contain	had	648:650	arg1	modifications					605:617	The qualitative/quantitative modifications	576:617	The qualitative/quantitative modifications of capsule shell composition	576:646	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	4	48	contain	had	648:650	arg2	impact					662:667	a limited impact	652:667	a limited impact	652:667	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	5	49	theme	capsule	913:919	arg1	size					930:933	wet capsule particle size	909:933	wet capsule particle size (about 4 mm)	909:946	Neither the type nor the amount of secondary excipient influenced wet capsule particle size (about 4 mm).					
32585275	4	50	theme	composition	636:646	arg1	modifications					605:617	The qualitative/quantitative modifications	576:617	The qualitative/quantitative modifications of capsule shell composition	576:646	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	4	51	theme	dried	752:756	arg1	properties					766:775	other dried capsule properties	746:775	other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior	746:840	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	4	52	theme	w/w	720:722	arg1	%					718:718	more than 50% w/w	706:722	more than 50% w/w	706:722	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	8	53	theme	maltodextrin	1284:1295	arg1	amount					1274:1279	the amount	1270:1279	the amount of maltodextrin included in the formulations	1270:1324	In addition, the amount of maltodextrin included in the formulations affected the swelling behavior of the dried capsules in water and this leads considering maltodextrin a useful excipient to modulate swelling according to the needs of the different applications.					
32585275	8	53	theme	maltodextrin	1284:1295	arg1	maltodextrin					1284:1295	maltodextrin	1284:1295	maltodextrin included in the formulations	1284:1324	In addition, the amount of maltodextrin included in the formulations affected the swelling behavior of the dried capsules in water and this leads considering maltodextrin a useful excipient to modulate swelling according to the needs of the different applications.					
32585275	4	54	theme	shell	630:634	arg1	composition					636:646	capsule shell composition	622:646	capsule shell composition	622:646	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	4	55	theme	shell	794:798	arg1	thickness					800:808	shell thickness	794:808	shell thickness	794:808	The qualitative/quantitative modifications of capsule shell composition had a limited impact on the essential oil content (it was more than 50% w/w), while they affected other dried capsule properties like dimensions, shell thickness, hardness and swelling behavior.					
32585275	1	56	theme	good	163:166	arg1	strategy					168:175	a good strategy	161:175	a good strategy to protect and to transform them in free-flowing particles	161:234	Encapsulation of essential oils represents a good strategy to protect and to transform them in free-flowing particles.					
32585275	2	57	contain	containing	256:265	arg2	oil					288:290	peppermint essential oil	267:290	peppermint essential oil	267:290	Core-shell systems containing peppermint essential oil have been obtained by inverse ionotropic gelation.					
32585275	2	57	contain	containing	256:265	arg1	systems					248:254	Core-shell systems	237:254	Core-shell systems containing peppermint essential oil	237:290	Core-shell systems containing peppermint essential oil have been obtained by inverse ionotropic gelation.					
32585275	3	58	theme	essential	371:379	arg1	O					386:386	O	386:386	O	386:386	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W) was dripped in an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations.					
32585275	3	58	theme	essential	371:379	arg1	oil					381:383	essential oil	371:383	essential oil (O)	371:387	An O/W emulsion composed of essential oil (O) and aqueous CaCl2 solution enriched with hydroxyethylcellulose (W) was dripped in an alginate solution added of a secondary excipient (maltodextrin or shellac gum) at two concentrations.					
32585275	2	59	theme	essential	278:286	arg1	oil					288:290	peppermint essential oil	267:290	peppermint essential oil	267:290	Core-shell systems containing peppermint essential oil have been obtained by inverse ionotropic gelation.					
32585275	7	60	theme	excipient	1226:1234	arg1	amount					1206:1211	the amount	1202:1211	the amount of secondary excipient in the formulations	1202:1254	Capsule hardness diminished by increasing the amount of secondary excipient in the formulations.					
32585275	7	60	theme	excipient	1226:1234	arg1	excipient					1226:1234	secondary excipient	1216:1234	secondary excipient	1216:1234	Capsule hardness diminished by increasing the amount of secondary excipient in the formulations.					
32585275	6	61	theme	dried	1051:1055	arg1	dimensions					1065:1074	dried capsule dimensions	1051:1074	dried capsule dimensions	1051:1074	After drying, particle size decreased (it was about 2 mm) and while shellac gum amount did not affect dried capsule dimensions, an increase in maltodextrin amount corresponded to an increase in their diameters.					
32585275	8	62	from	behavior	1348:1355	arg1	water					1382:1386	water	1382:1386	water	1382:1386	In addition, the amount of maltodextrin included in the formulations affected the swelling behavior of the dried capsules in water and this leads considering maltodextrin a useful excipient to modulate swelling according to the needs of the different applications.					
32585275	7	63	theme	Capsule	1160:1166	arg1	hardness					1168:1175	Capsule hardness	1160:1175	Capsule hardness	1160:1175	Capsule hardness diminished by increasing the amount of secondary excipient in the formulations.					
33080265	8	0	theme	propionic	1295:1303	arg1	acid					1305:1308	propionic acid	1295:1308	propionic acid	1295:1308	The formation of SCFAs was mainly acetic acid, followed by propionic acid and butyric acid, and the pH was decreased from 6.95 to 4.70.					
33080265	7	1	theme	In	1038:1039	arg1	fermentation					1047:1058	In vitro fermentation	1038:1058	In vitro fermentation of PNPS by gut microbiota	1038:1084	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	8	2	theme	acetic	1270:1275	arg1	acid					1277:1280	mainly acetic acid	1263:1280	mainly acetic acid	1263:1280	The formation of SCFAs was mainly acetic acid, followed by propionic acid and butyric acid, and the pH was decreased from 6.95 to 4.70.					
33080265	8	2	theme	acetic	1270:1275	arg1	formation					1240:1248	The formation	1236:1248	The formation of SCFAs	1236:1257	The formation of SCFAs was mainly acetic acid, followed by propionic acid and butyric acid, and the pH was decreased from 6.95 to 4.70.					
33080265	7	3	dep	significantly	1183:1195	arg1	p < 0.05					1198:1205	p < 0.05	1198:1205	p < 0.05	1198:1205	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	0	4	theme	in	92:93	arg1	characteristics					114:128	in vitro fermentation characteristics	92:128	in vitro fermentation characteristics	92:128	Polysaccharide from Pleurotus nebrodensis: Physicochemical, structural characterization and in vitro fermentation characteristics.					
33080265	7	5	theme	treatment	1122:1130	arg1	groups					1132:1137	all treatment groups	1118:1137	all treatment groups	1118:1137	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	9	6	theme	sugar	1394:1398	arg1	content					1400:1406	the total sugar content	1384:1406	the total sugar content	1384:1406	After 72 h, the total sugar content decreased from 5.813 ± 0.87 mg/L to 0.23 ± 0.065 mg/L, and the molecular weight of PNPS decreased.					
33080265	2	7	theme	polysaccharide	368:381	arg1	behaviors					350:358	in vitro fermentation behaviors	328:358	in vitro fermentation behaviors	328:358	The structural characteristics and in vitro fermentation behaviors of this polysaccharide was investigated.					
33080265	2	7	theme	polysaccharide	368:381	arg1	characteristics					308:322	The structural characteristics	293:322	The structural characteristics	293:322	The structural characteristics and in vitro fermentation behaviors of this polysaccharide was investigated.					
33080265	4	8	theme	mannose	617:623	arg1	percentage					603:612	small percentage	597:612	small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%)	597:675	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	4	9	theme	galactose	648:656	arg1	percentage					603:612	small percentage	597:612	small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%)	597:675	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	2	10	theme	structural	297:306	arg1	characteristics					308:322	The structural characteristics	293:322	The structural characteristics	293:322	The structural characteristics and in vitro fermentation behaviors of this polysaccharide was investigated.					
33080265	0	11	theme	fermentation	101:112	arg1	characteristics					114:128	in vitro fermentation characteristics	92:128	in vitro fermentation characteristics	92:128	Polysaccharide from Pleurotus nebrodensis: Physicochemical, structural characterization and in vitro fermentation characteristics.					
33080265	4	12	theme	composition	513:523	arg1	analysis					525:532	Monosaccharide composition analysis	498:532	Monosaccharide composition analysis	498:532	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	3	13	dep	showed	431:436	arg1	%					495:495	up to 97.20 ± 1.80 wt%	474:495	up to 97.20 ± 1.80 wt%	474:495	Chemical composition analysis showed the total sugar content of PNPS was up to 97.20 ± 1.80 wt%.					
33080265	1	14	theme	ethanol	270:276	arg1	precipitation					278:290	ethanol precipitation	270:290	ethanol precipitation	270:290	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	5	15	theme	58.90 mol	797:805	arg1	4-β-D-Glcp					785:794	4-β-D-Glcp	785:794	4-β-D-Glcp (58.90 mol%)	785:807	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	5	15	theme	58.90 mol	797:805	arg1	%					806:806	58.90 mol%	797:806	58.90 mol%	797:806	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	4	16	theme	Monosaccharide	498:511	arg1	analysis					525:532	Monosaccharide composition analysis	498:532	Monosaccharide composition analysis	498:532	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	7	17	theme	total	1093:1097	arg1	production					1104:1113	total SCFA production	1093:1113	total SCFA production of all treatment groups	1093:1137	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	8	18	dep	4.70	1366:1369	arg1	to					1363:1364	to	1363:1364	to	1363:1364	The formation of SCFAs was mainly acetic acid, followed by propionic acid and butyric acid, and the pH was decreased from 6.95 to 4.70.					
33080265	4	19	theme	5.60 ± 0.74 mol	626:640	arg1	mannose					617:623	mannose	617:623	mannose (5.60 ± 0.74 mol%)	617:642	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	4	19	theme	5.60 ± 0.74 mol	626:640	arg1	%					641:641	5.60 ± 0.74 mol%	626:641	5.60 ± 0.74 mol%	626:641	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	7	20	theme	SCFA	1099:1102	arg1	production					1104:1113	total SCFA production	1093:1113	total SCFA production of all treatment groups	1093:1137	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	2	21	theme	fermentation	337:348	arg1	behaviors					350:358	in vitro fermentation behaviors	328:358	in vitro fermentation behaviors	328:358	The structural characteristics and in vitro fermentation behaviors of this polysaccharide was investigated.					
33080265	5	22	theme	other	816:820	arg1	6-α-D-Galp					851:860	6-α-D-Galp	851:860	6-α-D-Galp	851:860	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	5	22	theme	other	816:820	arg1	3,6-α-D-Manp					863:874	3,6-α-D-Manp	863:874	3,6-α-D-Manp	863:874	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	5	22	theme	other	816:820	arg1	residues					822:829	other residues	816:829	other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp	816:901	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	5	22	theme	other	816:820	arg1	α-D-Glcp					841:848	α-D-Glcp	841:848	α-D-Glcp	841:848	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	5	22	theme	other	816:820	arg1	3-β-D-Glcp					877:886	3-β-D-Glcp	877:886	3-β-D-Glcp	877:886	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	5	22	theme	other	816:820	arg1	6-α-D-Glcp					892:901	6-α-D-Glcp	892:901	6-α-D-Glcp	892:901	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	1	23	theme	5012 kDa	142:149	arg1	polysaccharide					152:165	A high Mw (5012 kDa) polysaccharide	131:165	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis	131:220	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	1	23	theme	5012 kDa	142:149	arg1	PNPS					168:171	PNPS	168:171	PNPS	168:171	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	4	24	dep	showed	534:539	arg1	contained					546:554	contained	546:554	showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected	534:694	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	9	25	theme	total	1388:1392	arg1	content					1400:1406	the total sugar content	1384:1406	the total sugar content	1384:1406	After 72 h, the total sugar content decreased from 5.813 ± 0.87 mg/L to 0.23 ± 0.065 mg/L, and the molecular weight of PNPS decreased.					
33080265	4	26	theme	small	597:601	arg1	percentage					603:612	small percentage	597:612	small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%)	597:675	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	7	27	theme	PNPS	1063:1066	arg1	fermentation					1047:1058	In vitro fermentation	1038:1058	In vitro fermentation of PNPS by gut microbiota	1038:1084	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	2	28	theme	in	328:329	arg1	behaviors					350:358	in vitro fermentation behaviors	328:358	in vitro fermentation behaviors	328:358	The structural characteristics and in vitro fermentation behaviors of this polysaccharide was investigated.					
33080265	1	29	theme	fruiting	183:190	arg1	body					192:195	the fruiting body	179:195	the fruiting body of Pleurotus nebrodensis	179:220	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	3	30	theme	Chemical	401:408	arg1	analysis					422:429	Chemical composition analysis	401:429	Chemical composition analysis	401:429	Chemical composition analysis showed the total sugar content of PNPS was up to 97.20 ± 1.80 wt%.					
33080265	6	31	theme	NMR	980:982	arg1	spectrum					984:991	NMR spectrum	980:991	NMR spectrum	980:991	Combined with results from 1D and 2D NMR spectrum, a proposed structure of PNPS was presented.					
33080265	6	32	from	2D	977:978	arg1	results					957:963	results	957:963	results from 1D and 2D NMR spectrum	957:991	Combined with results from 1D and 2D NMR spectrum, a proposed structure of PNPS was presented.					
33080265	7	33	dep	In	1038:1039	arg1	vitro					1041:1045	vitro	1041:1045	vitro	1041:1045	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	3	34	theme	composition	410:420	arg1	analysis					422:429	Chemical composition analysis	401:429	Chemical composition analysis	401:429	Chemical composition analysis showed the total sugar content of PNPS was up to 97.20 ± 1.80 wt%.					
33080265	7	35	theme	gut	1071:1073	arg1	microbiota					1075:1084	gut microbiota	1071:1084	gut microbiota	1071:1084	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	7	36	theme	fermentation	1222:1233	arg1	48 h					1214:1217	48 h	1214:1217	48 h of fermentation	1214:1233	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	4	37	theme	5.18 ± 0.33 mol	659:673	arg1	galactose					648:656	galactose	648:656	galactose (5.18 ± 0.33 mol%)	648:675	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	4	37	theme	5.18 ± 0.33 mol	659:673	arg1	%					674:674	5.18 ± 0.33 mol%	659:674	5.18 ± 0.33 mol%	659:674	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	3	38	theme	sugar	448:452	arg1	content					454:460	the total sugar content	438:460	the total sugar content of PNPS	438:468	Chemical composition analysis showed the total sugar content of PNPS was up to 97.20 ± 1.80 wt%.					
33080265	5	39	theme	methylation	740:750	arg1	analysis					730:737	the linkage pattern analysis	710:737	the linkage pattern analysis (methylation analysis)	710:760	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	5	39	theme	methylation	740:750	arg1	analysis					752:759	methylation analysis	740:759	methylation analysis	740:759	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	3	40	theme	PNPS	465:468	arg1	content					454:460	the total sugar content	438:460	the total sugar content of PNPS	438:468	Chemical composition analysis showed the total sugar content of PNPS was up to 97.20 ± 1.80 wt%.					
33080265	7	41	dep	showed	1086:1091	arg1	higher					1143:1148	higher	1143:1148	higher	1143:1148	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	4	42	theme	89.22 ± 5.70 mol	572:587	arg1	%					588:588	89.22 ± 5.70 mol%	572:588	89.22 ± 5.70 mol%	572:588	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	4	42	theme	89.22 ± 5.70 mol	572:587	arg1	glucose					563:569	glucose	563:569	mainly glucose (89.22 ± 5.70 mol%)	556:589	Monosaccharide composition analysis showed PNPS contained mainly glucose (89.22 ± 5.70 mol%) while small percentage of mannose (5.60 ± 0.74 mol%) and galactose (5.18 ± 0.33 mol%) were also detected.					
33080265	6	43	theme	proposed	996:1003	arg1	structure					1005:1013	a proposed structure	994:1013	a proposed structure of PNPS	994:1021	Combined with results from 1D and 2D NMR spectrum, a proposed structure of PNPS was presented.					
33080265	6	44	dep	1D	970:971	arg1	spectrum					984:991	NMR spectrum	980:991	NMR spectrum	980:991	Combined with results from 1D and 2D NMR spectrum, a proposed structure of PNPS was presented.					
33080265	0	45	dep	in	92:93	arg1	vitro					95:99	vitro	95:99	vitro	95:99	Polysaccharide from Pleurotus nebrodensis: Physicochemical, structural characterization and in vitro fermentation characteristics.					
33080265	1	46	theme	nebrodensis	210:220	arg1	body					192:195	the fruiting body	179:195	the fruiting body of Pleurotus nebrodensis	179:220	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	0	47	theme	structural	60:69	arg1	characterization					71:86	structural characterization	60:86	structural characterization	60:86	Polysaccharide from Pleurotus nebrodensis: Physicochemical, structural characterization and in vitro fermentation characteristics.					
33080265	8	48	theme	butyric	1314:1320	arg1	acid					1322:1325	butyric acid	1314:1325	butyric acid	1314:1325	The formation of SCFAs was mainly acetic acid, followed by propionic acid and butyric acid, and the pH was decreased from 6.95 to 4.70.					
33080265	6	49	from	1D	970:971	arg1	results					957:963	results	957:963	results from 1D and 2D NMR spectrum	957:991	Combined with results from 1D and 2D NMR spectrum, a proposed structure of PNPS was presented.					
33080265	1	50	theme	high	133:136	arg1	polysaccharide					152:165	A high Mw (5012 kDa) polysaccharide	131:165	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis	131:220	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	1	50	theme	high	133:136	arg1	PNPS					168:171	PNPS	168:171	PNPS	168:171	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	3	51	theme	total	442:446	arg1	content					454:460	the total sugar content	438:460	the total sugar content of PNPS	438:468	Chemical composition analysis showed the total sugar content of PNPS was up to 97.20 ± 1.80 wt%.					
33080265	9	52	theme	PNPS	1491:1494	arg1	weight					1481:1486	the molecular weight	1467:1486	the molecular weight of PNPS	1467:1494	After 72 h, the total sugar content decreased from 5.813 ± 0.87 mg/L to 0.23 ± 0.065 mg/L, and the molecular weight of PNPS decreased.					
33080265	1	53	from	body	192:195	arg1	polysaccharide					152:165	A high Mw (5012 kDa) polysaccharide	131:165	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis	131:220	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	1	53	from	body	192:195	arg1	PNPS					168:171	PNPS	168:171	PNPS	168:171	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	1	54	theme	Mw	138:139	arg1	polysaccharide					152:165	A high Mw (5012 kDa) polysaccharide	131:165	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis	131:220	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	1	54	theme	Mw	138:139	arg1	PNPS					168:171	PNPS	168:171	PNPS	168:171	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
33080265	7	55	theme	negative	1155:1162	arg1	NC					1179:1180	NC	1179:1180	NC	1179:1180	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	7	55	theme	negative	1155:1162	arg1	group					1172:1176	negative control group	1155:1176	negative control group (NC)	1155:1181	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	5	56	theme	linkage	714:720	arg1	analysis					730:737	the linkage pattern analysis	710:737	the linkage pattern analysis (methylation analysis)	710:760	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	5	56	theme	linkage	714:720	arg1	analysis					752:759	methylation analysis	740:759	methylation analysis	740:759	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	9	57	theme	molecular	1471:1479	arg1	weight					1481:1486	the molecular weight	1467:1486	the molecular weight of PNPS	1467:1494	After 72 h, the total sugar content decreased from 5.813 ± 0.87 mg/L to 0.23 ± 0.065 mg/L, and the molecular weight of PNPS decreased.					
33080265	6	58	with	Combined	943:950	arg1	results					957:963	results	957:963	results from 1D and 2D NMR spectrum	957:991	Combined with results from 1D and 2D NMR spectrum, a proposed structure of PNPS was presented.					
33080265	5	59	theme	pattern	722:728	arg1	analysis					730:737	the linkage pattern analysis	710:737	the linkage pattern analysis (methylation analysis)	710:760	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	5	59	theme	pattern	722:728	arg1	analysis					752:759	methylation analysis	740:759	methylation analysis	740:759	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	5	60	theme	comparable	924:933	arg1	amount					935:940	a comparable amount	922:940	a comparable amount	922:940	According to the linkage pattern analysis (methylation analysis), PNPS comprised mainly 4-β-D-Glcp (58.90 mol%), while other residues including α-D-Glcp, 6-α-D-Galp, 3,6-α-D-Manp, 3-β-D-Glcp and 6-α-D-Glcp were detected with a comparable amount.					
33080265	3	61	dep	97.20 ± 1.80 wt	480:494	arg1	to					477:478	to	477:478	to	477:478	Chemical composition analysis showed the total sugar content of PNPS was up to 97.20 ± 1.80 wt%.					
33080265	2	62	dep	in	328:329	arg1	vitro					331:335	vitro	331:335	vitro	331:335	The structural characteristics and in vitro fermentation behaviors of this polysaccharide was investigated.					
33080265	8	63	theme	SCFAs	1253:1257	arg1	acid					1277:1280	mainly acetic acid	1263:1280	mainly acetic acid	1263:1280	The formation of SCFAs was mainly acetic acid, followed by propionic acid and butyric acid, and the pH was decreased from 6.95 to 4.70.					
33080265	8	63	theme	SCFAs	1253:1257	arg1	formation					1240:1248	The formation	1236:1248	The formation of SCFAs	1236:1257	The formation of SCFAs was mainly acetic acid, followed by propionic acid and butyric acid, and the pH was decreased from 6.95 to 4.70.					
33080265	6	64	theme	PNPS	1018:1021	arg1	structure					1005:1013	a proposed structure	994:1013	a proposed structure of PNPS	994:1021	Combined with results from 1D and 2D NMR spectrum, a proposed structure of PNPS was presented.					
33080265	7	65	theme	groups	1132:1137	arg1	production					1104:1113	total SCFA production	1093:1113	total SCFA production of all treatment groups	1093:1137	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	0	66	from	nebrodensis	30:40	arg1	Polysaccharide					0:13	Polysaccharide	0:13	Polysaccharide from Pleurotus nebrodensis: Physicochemical, structural characterization and in vitro fermentation characteristics.	0:129	Polysaccharide from Pleurotus nebrodensis: Physicochemical, structural characterization and in vitro fermentation characteristics.					
33080265	0	67	dep	Polysaccharide	0:13	arg1	Physicochemical					43:57	Physicochemical	43:57	Physicochemical	43:57	Polysaccharide from Pleurotus nebrodensis: Physicochemical, structural characterization and in vitro fermentation characteristics.					
33080265	0	67	dep	Polysaccharide	0:13	arg1	characterization					71:86	structural characterization	60:86	structural characterization	60:86	Polysaccharide from Pleurotus nebrodensis: Physicochemical, structural characterization and in vitro fermentation characteristics.					
33080265	0	67	dep	Polysaccharide	0:13	arg1	characteristics					114:128	in vitro fermentation characteristics	92:128	in vitro fermentation characteristics	92:128	Polysaccharide from Pleurotus nebrodensis: Physicochemical, structural characterization and in vitro fermentation characteristics.					
33080265	7	68	theme	control	1164:1170	arg1	NC					1179:1180	NC	1179:1180	NC	1179:1180	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	7	68	theme	control	1164:1170	arg1	group					1172:1176	negative control group	1155:1176	negative control group (NC)	1155:1181	In vitro fermentation of PNPS by gut microbiota showed total SCFA production of all treatment groups was higher than negative control group (NC) significantly (p < 0.05) after 48 h of fermentation.					
33080265	1	69	theme	water	241:245	arg1	extraction					247:256	water extraction	241:256	water extraction followed by ethanol precipitation	241:290	A high Mw (5012 kDa) polysaccharide (PNPS) from the fruiting body of Pleurotus nebrodensis was isolated using water extraction followed by ethanol precipitation.					
34921890	3	0	theme	С6	703:704	arg1	lines					711:715	С6 cell lines	703:715	С6 cell lines	703:715	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	2	1	theme	animal	385:390	arg1	HeLa					424:427	HeLa	424:427	HeLa	424:427	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	1	theme	animal	385:390	arg1	Hep-2					417:421	Hep-2	417:421	Hep-2	417:421	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	1	theme	animal	385:390	arg1	A549					411:414	A549	411:414	A549	411:414	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	1	theme	animal	385:390	arg1	SPEV-2					437:442	SPEV-2	437:442	SPEV-2	437:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	1	theme	animal	385:390	arg1	lines					404:408	several human and animal cancer cell lines	367:408	several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2	367:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	2	attach	isolated	449:456	arg2	fractions					323:331	The glycoprotein and polysaccharide fractions	287:331	fractions	323:331	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	2	attach	isolated	449:456	arg1	body					526:529	fruiting body	517:529	fruiting body	517:529	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	2	attach	isolated	449:456	arg1	mycelium					504:511	basidiomycete Lentinus edodes vegetative mycelium	463:511	basidiomycete Lentinus edodes vegetative mycelium	463:511	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	8	3	theme	particular	1460:1469	arg1	tools					1576:1580	novel prospective tools	1558:1580	novel prospective tools in cancer diagnostics and therapies	1558:1616	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	8	3	theme	particular	1460:1469	arg1	preparations					1479:1490	Fungal protein and, in particular, lectin preparations	1437:1490	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium	1437:1533	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	2	4	gly	glycoprotein	291:302	arg1	glycoprotein					291:302	The glycoprotein and polysaccharide fractions	287:331	glycoprotein	291:302	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	5	5	theme	protein	838:844	arg1	composition					858:868	The protein and subunit composition	834:868	composition	858:868	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	7	6	from	mucosa	1359:1364	arg1	administration					1388:1401	peroral administration	1380:1401	peroral administration of fungal glycoprotein fractions	1380:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	7	6	from	mucosa	1359:1364	arg1	system					1288:1293	immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1281:1434	system	1288:1293	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	7	6	from	mucosa	1359:1364	arg1	absence					1314:1320	the absence	1310:1320	the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1310:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	2	7	theme	high	341:344	arg1	cytotoxicity					346:357	high cytotoxicity	341:357	high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2	341:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	1	8	theme	compounds	205:213	arg1	Detection					162:170	Detection	162:170	Detection	162:170	Detection and study of biologically active compounds seems a promising area of research in cancer diagnostics and therapies.					
34921890	1	8	theme	compounds	205:213	arg1	study					176:180	study	176:180	study	176:180	Detection and study of biologically active compounds seems a promising area of research in cancer diagnostics and therapies.					
34921890	6	9	theme	morphogenesis	1199:1211	arg1	stage					1176:1180	each stage	1171:1180	each stage of basidiomycete morphogenesis	1171:1211	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	0	10	theme	animal	136:141	arg1	lines					155:159	human and animal cancer cell lines	126:159	human and animal cancer cell lines	126:159	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	3	11	theme	water-soluble	576:588	arg1	fractions					603:611	water-soluble glycoprotein fractions	576:611	water-soluble glycoprotein fractions	576:611	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	6	12	theme	Comparative	986:996	arg1	analysis					998:1005	Comparative analysis	986:1005	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium	986:1086	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	0	13	theme	cell	150:153	arg1	lines					155:159	human and animal cancer cell lines	126:159	human and animal cancer cell lines	126:159	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	3	14	theme	significant	629:639	arg1	inhibition					650:659	70-100% inhibition	642:659	70-100% inhibition	642:659	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	7	15	from	system	1288:1293	arg1	mucosa					1359:1364	gastro-intestinal mucosa	1341:1364	gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1341:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	4	16	theme	effective	722:730	arg1	concentrations					732:745	The effective concentrations	718:745	The effective concentrations of glycoprotein fractions reducing the viability of cancer cell lines	718:815	The effective concentrations of glycoprotein fractions reducing the viability of cancer cell lines were determined.					
34921890	8	17	theme	prospective	1564:1574	arg1	tools					1576:1580	novel prospective tools	1558:1580	novel prospective tools in cancer diagnostics and therapies	1558:1616	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	8	17	theme	prospective	1564:1574	arg1	preparations					1479:1490	Fungal protein and, in particular, lectin preparations	1437:1490	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium	1437:1533	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	6	18	theme	fruiting	1059:1066	arg1	transcriptomes					1010:1023	transcriptomes	1010:1023	transcriptomes of L. edodes vegetative mycelium	1010:1056	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	6	18	theme	fruiting	1059:1066	arg1	body					1068:1071	fruiting body	1059:1071	fruiting body	1059:1071	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	2	19	theme	fruiting	517:524	arg1	body					526:529	fruiting body	517:529	fruiting body	517:529	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	7	20	from	absence	1314:1320	arg1	mucosa					1359:1364	gastro-intestinal mucosa	1341:1364	gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1341:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	0	21	theme	basidiomycete	67:79	arg1	edodes					90:95	medicinal basidiomycete Lentinus edodes	57:95	medicinal basidiomycete Lentinus edodes	57:95	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	3	22	theme	70-100	642:647	arg1	%					648:648	%	648:648	%	648:648	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	5	23	theme	galactose-specific	914:931	arg1	lectins					933:939	the highly active galactose-specific lectins	896:939	the highly active galactose-specific lectins	896:939	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	5	23	theme	galactose-specific	914:931	arg1	present					958:964	present	958:964	present	958:964	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	6	24	theme	mycelium	1049:1056	arg1	body					1068:1071	fruiting body	1059:1071	fruiting body	1059:1071	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	6	24	theme	mycelium	1049:1056	arg1	primordium					1077:1086	primordium	1077:1086	primordium	1077:1086	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	6	24	theme	mycelium	1049:1056	arg1	transcriptomes					1010:1023	transcriptomes	1010:1023	transcriptomes of L. edodes vegetative mycelium	1010:1056	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	0	25	theme	edodes	90:95	arg1	mycelium					45:52	vegetative mycelium	34:52	vegetative mycelium of medicinal basidiomycete Lentinus edodes	34:95	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	5	26	located	present	958:964	arg1	fractions					975:983	these fractions	969:983	these fractions	969:983	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	5	26	located	present	958:964	arg2	present					958:964	present	958:964	present	958:964	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	5	26	located	present	958:964	arg2	lectins					933:939	the highly active galactose-specific lectins	896:939	the highly active galactose-specific lectins	896:939	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	6	27	theme	edodes	1031:1036	arg1	mycelium					1049:1056	L. edodes vegetative mycelium	1028:1056	L. edodes vegetative mycelium	1028:1056	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	8	28	theme	protein	1444:1450	arg1	tools					1576:1580	novel prospective tools	1558:1580	novel prospective tools in cancer diagnostics and therapies	1558:1616	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	8	28	theme	protein	1444:1450	arg1	preparations					1479:1490	Fungal protein and, in particular, lectin preparations	1437:1490	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium	1437:1533	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	7	29	theme	system	1288:1293	arg1	indicators					1267:1276	some morphological indicators	1248:1276	some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1248:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	4	30	theme	cell	806:809	arg1	lines					811:815	cancer cell lines	799:815	cancer cell lines	799:815	The effective concentrations of glycoprotein fractions reducing the viability of cancer cell lines were determined.					
34921890	0	31	theme	human	126:130	arg1	lines					155:159	human and animal cancer cell lines	126:159	human and animal cancer cell lines	126:159	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	6	32	theme	carbohydrate-binding	1113:1132	arg1	lectins					1149:1155	lectins	1149:1155	lectins	1149:1155	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	6	32	theme	carbohydrate-binding	1113:1132	arg1	glycoproteins					1134:1146	carbohydrate-binding glycoproteins	1113:1146	carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis	1113:1211	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	4	33	theme	fractions	763:771	arg1	concentrations					732:745	The effective concentrations	718:745	The effective concentrations of glycoprotein fractions reducing the viability of cancer cell lines	718:815	The effective concentrations of glycoprotein fractions reducing the viability of cancer cell lines were determined.					
34921890	7	34	theme	morphological	1253:1265	arg1	indicators					1267:1276	some morphological indicators	1248:1276	some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1248:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	7	35	theme	glycoprotein	1413:1424	arg1	fractions					1426:1434	fungal glycoprotein fractions	1406:1434	fungal glycoprotein fractions	1406:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	8	36	theme	lectin	1472:1477	arg1	tools					1576:1580	novel prospective tools	1558:1580	novel prospective tools in cancer diagnostics and therapies	1558:1616	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	8	36	theme	lectin	1472:1477	arg1	preparations					1479:1490	Fungal protein and, in particular, lectin preparations	1437:1490	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium	1437:1533	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	5	37	theme	subunit	850:856	arg1	composition					858:868	The protein and subunit composition	834:868	composition	858:868	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	2	38	theme	edodes	486:491	arg1	mycelium					504:511	basidiomycete Lentinus edodes vegetative mycelium	463:511	basidiomycete Lentinus edodes vegetative mycelium	463:511	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	0	39	theme	vegetative	34:43	arg1	mycelium					45:52	vegetative mycelium	34:52	vegetative mycelium of medicinal basidiomycete Lentinus edodes	34:95	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	7	40	theme	Histological	1214:1225	arg1	examination					1227:1237	Histological examination	1214:1237	Histological examination	1214:1237	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	7	41	theme	peroral	1380:1386	arg1	administration					1388:1401	peroral administration	1380:1401	peroral administration of fungal glycoprotein fractions	1380:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	2	42	theme	basidiomycete	463:475	arg1	mycelium					504:511	basidiomycete Lentinus edodes vegetative mycelium	463:511	basidiomycete Lentinus edodes vegetative mycelium	463:511	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	3	43	theme	metabolic	664:672	arg1	activity					674:681	metabolic activity	664:681	metabolic activity of SPЕV-2, А549 and С6 cell lines	664:715	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	8	44	theme	L.	1505:1506	arg1	mycelium					1526:1533	L. еdodes vegetative mycelium	1505:1533	L. еdodes vegetative mycelium	1505:1533	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	7	45	from	administration	1388:1401	arg1	mucosa					1359:1364	gastro-intestinal mucosa	1341:1364	gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1341:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	7	45	from	administration	1388:1401	arg1	animals					1369:1375	animals	1369:1375	animals at peroral administration of fungal glycoprotein fractions	1369:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	7	46	theme	animals	1369:1375	arg1	mucosa					1359:1364	gastro-intestinal mucosa	1341:1364	gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1341:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	8	47	theme	vegetative	1515:1524	arg1	mycelium					1526:1533	L. еdodes vegetative mycelium	1505:1533	L. еdodes vegetative mycelium	1505:1533	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	2	48	theme	several	367:373	arg1	HeLa					424:427	HeLa	424:427	HeLa	424:427	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	48	theme	several	367:373	arg1	Hep-2					417:421	Hep-2	417:421	Hep-2	417:421	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	48	theme	several	367:373	arg1	A549					411:414	A549	411:414	A549	411:414	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	48	theme	several	367:373	arg1	SPEV-2					437:442	SPEV-2	437:442	SPEV-2	437:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	48	theme	several	367:373	arg1	lines					404:408	several human and animal cancer cell lines	367:408	several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2	367:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	49	theme	cancer	392:397	arg1	HeLa					424:427	HeLa	424:427	HeLa	424:427	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	49	theme	cancer	392:397	arg1	Hep-2					417:421	Hep-2	417:421	Hep-2	417:421	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	49	theme	cancer	392:397	arg1	A549					411:414	A549	411:414	A549	411:414	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	49	theme	cancer	392:397	arg1	SPEV-2					437:442	SPEV-2	437:442	SPEV-2	437:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	49	theme	cancer	392:397	arg1	lines					404:408	several human and animal cancer cell lines	367:408	several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2	367:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	5	50	attach	present	958:964	arg1	fractions					975:983	these fractions	969:983	these fractions	969:983	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	5	50	attach	present	958:964	arg2	present					958:964	present	958:964	present	958:964	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	5	50	attach	present	958:964	arg2	lectins					933:939	the highly active galactose-specific lectins	896:939	the highly active galactose-specific lectins	896:939	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	7	51	theme	toxic	1325:1329	arg1	effect					1331:1336	toxic effect	1325:1336	toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1325:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	3	52	theme	cell	706:709	arg1	lines					711:715	С6 cell lines	703:715	С6 cell lines	703:715	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	2	53	theme	polysaccharide	308:321	arg1	fractions					323:331	The glycoprotein and polysaccharide fractions	287:331	fractions	323:331	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	7	54	from	effect	1331:1336	arg1	mucosa					1359:1364	gastro-intestinal mucosa	1341:1364	gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1341:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	2	55	theme	glycoprotein	291:302	arg1	fractions					323:331	The glycoprotein and polysaccharide fractions	287:331	fractions	323:331	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	8	56	theme	еdodes	1508:1513	arg1	mycelium					1526:1533	L. еdodes vegetative mycelium	1505:1533	L. еdodes vegetative mycelium	1505:1533	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	3	57	theme	glycoprotein	590:601	arg1	fractions					603:611	water-soluble glycoprotein fractions	576:611	water-soluble glycoprotein fractions	576:611	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	1	58	theme	active	198:203	arg1	compounds					205:213	biologically active compounds	185:213	biologically active compounds	185:213	Detection and study of biologically active compounds seems a promising area of research in cancer diagnostics and therapies.					
34921890	6	59	theme	transcriptomes	1010:1023	arg1	analysis					998:1005	Comparative analysis	986:1005	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium	986:1086	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	0	60	theme	cancer	143:148	arg1	lines					155:159	human and animal cancer cell lines	126:159	human and animal cancer cell lines	126:159	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	6	61	theme	basidiomycete	1185:1197	arg1	morphogenesis					1199:1211	basidiomycete morphogenesis	1185:1211	basidiomycete morphogenesis	1185:1211	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	5	62	theme	fractions	873:881	arg1	composition					858:868	The protein and subunit composition	834:868	composition	858:868	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	8	63	theme	novel	1558:1562	arg1	tools					1576:1580	novel prospective tools	1558:1580	novel prospective tools in cancer diagnostics and therapies	1558:1616	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	8	63	theme	novel	1558:1562	arg1	preparations					1479:1490	Fungal protein and, in particular, lectin preparations	1437:1490	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium	1437:1533	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	7	64	theme	immune	1281:1286	arg1	system					1288:1293	immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1281:1434	system	1288:1293	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	1	65	from	area	233:236	arg1	therapies					276:284	therapies	276:284	therapies	276:284	Detection and study of biologically active compounds seems a promising area of research in cancer diagnostics and therapies.					
34921890	1	65	from	area	233:236	arg1	diagnostics					260:270	cancer diagnostics	253:270	cancer diagnostics	253:270	Detection and study of biologically active compounds seems a promising area of research in cancer diagnostics and therapies.					
34921890	3	66	gly	glycoprotein	590:601	arg1	glycoprotein					590:601	water-soluble glycoprotein fractions	576:611	water-soluble glycoprotein fractions	576:611	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	3	67	theme	%	648:648	arg1	inhibition					650:659	70-100% inhibition	642:659	70-100% inhibition	642:659	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	8	68	theme	cancer	1585:1590	arg1	diagnostics					1592:1602	cancer diagnostics	1585:1602	cancer diagnostics	1585:1602	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	0	69	theme	Lentinus	81:88	arg1	edodes					90:95	medicinal basidiomycete Lentinus edodes	57:95	medicinal basidiomycete Lentinus edodes	57:95	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	5	70	theme	active	907:912	arg1	lectins					933:939	the highly active galactose-specific lectins	896:939	the highly active galactose-specific lectins	896:939	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	5	70	theme	active	907:912	arg1	present					958:964	present	958:964	present	958:964	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	6	71	theme	vegetative	1038:1047	arg1	mycelium					1049:1056	L. edodes vegetative mycelium	1028:1056	L. edodes vegetative mycelium	1028:1056	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	2	72	theme	vegetative	493:502	arg1	mycelium					504:511	basidiomycete Lentinus edodes vegetative mycelium	463:511	basidiomycete Lentinus edodes vegetative mycelium	463:511	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	4	73	gly	glycoprotein	750:761	arg1	glycoprotein					750:761	glycoprotein fractions	750:771	glycoprotein fractions reducing the viability of cancer cell lines	750:815	The effective concentrations of glycoprotein fractions reducing the viability of cancer cell lines were determined.					
34921890	7	74	theme	fractions	1426:1434	arg1	administration					1388:1401	peroral administration	1380:1401	peroral administration of fungal glycoprotein fractions	1380:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	6	75	theme	L.	1028:1029	arg1	mycelium					1049:1056	L. edodes vegetative mycelium	1028:1056	L. edodes vegetative mycelium	1028:1056	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	8	76	theme	Fungal	1437:1442	arg1	protein					1444:1450	Fungal protein	1437:1450	Fungal protein	1437:1450	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	8	77	from	tools	1576:1580	arg1	therapies					1608:1616	therapies	1608:1616	therapies	1608:1616	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	8	77	from	tools	1576:1580	arg1	diagnostics					1592:1602	cancer diagnostics	1585:1602	cancer diagnostics	1585:1602	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	6	78	theme	glycoproteins	1134:1146	arg1	presence					1101:1108	the presence	1097:1108	the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis	1097:1211	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	0	79	from	mycelium	45:52	arg1	polysaccharides					13:27	polysaccharides	13:27	polysaccharides	13:27	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	0	79	from	mycelium	45:52	arg1	Proteins					0:7	Proteins	0:7	Proteins	0:7	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	4	80	theme	cancer	799:804	arg1	lines					811:815	cancer cell lines	799:815	cancer cell lines	799:815	The effective concentrations of glycoprotein fractions reducing the viability of cancer cell lines were determined.					
34921890	8	81	attach	derived	1492:1498	arg2	preparations					1479:1490	Fungal protein and, in particular, lectin preparations	1437:1490	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium	1437:1533	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	8	81	attach	derived	1492:1498	arg2	tools					1576:1580	novel prospective tools	1558:1580	novel prospective tools in cancer diagnostics and therapies	1558:1616	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	8	81	attach	derived	1492:1498	arg1	mycelium					1526:1533	L. еdodes vegetative mycelium	1505:1533	L. еdodes vegetative mycelium	1505:1533	Fungal protein and, in particular, lectin preparations derived from L. еdodes vegetative mycelium might be considered as novel prospective tools in cancer diagnostics and therapies.					
34921890	7	82	gly	glycoprotein	1413:1424	arg1	glycoprotein					1413:1424	fungal glycoprotein fractions	1406:1434	fungal glycoprotein fractions	1406:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	5	83	from	present	958:964	arg1	fractions					975:983	these fractions	969:983	these fractions	969:983	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	7	84	theme	fungal	1406:1411	arg1	fractions					1426:1434	fungal glycoprotein fractions	1406:1434	fungal glycoprotein fractions	1406:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	4	85	theme	glycoprotein	750:761	arg1	fractions					763:771	glycoprotein fractions	750:771	glycoprotein fractions reducing the viability of cancer cell lines	750:815	The effective concentrations of glycoprotein fractions reducing the viability of cancer cell lines were determined.					
34921890	2	86	dep	lines	404:408	arg1	Hep-2					417:421	Hep-2	417:421	Hep-2	417:421	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	86	dep	lines	404:408	arg1	SPEV-2					437:442	SPEV-2	437:442	SPEV-2	437:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	86	dep	lines	404:408	arg1	lines					404:408	several human and animal cancer cell lines	367:408	several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2	367:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	86	dep	lines	404:408	arg1	HeLa					424:427	HeLa	424:427	HeLa	424:427	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	86	dep	lines	404:408	arg1	С6					430:431	С6	430:431	С6	430:431	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	86	dep	lines	404:408	arg1	A549					411:414	A549	411:414	A549	411:414	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	3	87	theme	activity	674:681	arg1	inhibition					650:659	70-100% inhibition	642:659	70-100% inhibition	642:659	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	6	88	theme	specific	1158:1165	arg1	lectins					1149:1155	lectins	1149:1155	lectins	1149:1155	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	6	88	theme	specific	1158:1165	arg1	glycoproteins					1134:1146	carbohydrate-binding glycoproteins	1113:1146	carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis	1113:1211	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	1	89	theme	promising	223:231	arg1	area					233:236	a promising area	221:236	a promising area of research in cancer diagnostics and therapies	221:284	Detection and study of biologically active compounds seems a promising area of research in cancer diagnostics and therapies.					
34921890	2	90	theme	Lentinus	477:484	arg1	mycelium					504:511	basidiomycete Lentinus edodes vegetative mycelium	463:511	basidiomycete Lentinus edodes vegetative mycelium	463:511	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	0	91	theme	medicinal	57:65	arg1	edodes					90:95	medicinal basidiomycete Lentinus edodes	57:95	medicinal basidiomycete Lentinus edodes	57:95	Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.					
34921890	6	92	gly	glycoproteins	1134:1146	arg1	lectins					1149:1155	lectins	1149:1155	lectins	1149:1155	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	6	92	gly	glycoproteins	1134:1146	arg1	glycoproteins					1134:1146	carbohydrate-binding glycoproteins	1113:1146	carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis	1113:1211	Comparative analysis of transcriptomes of L. edodes vegetative mycelium, fruiting body and primordium revealed the presence of carbohydrate-binding glycoproteins (lectins) specific for each stage of basidiomycete morphogenesis.					
34921890	3	93	theme	А549	694:697	arg1	activity					674:681	metabolic activity	664:681	metabolic activity of SPЕV-2, А549 and С6 cell lines	664:715	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	2	94	theme	human	375:379	arg1	HeLa					424:427	HeLa	424:427	HeLa	424:427	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	94	theme	human	375:379	arg1	Hep-2					417:421	Hep-2	417:421	Hep-2	417:421	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	94	theme	human	375:379	arg1	A549					411:414	A549	411:414	A549	411:414	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	94	theme	human	375:379	arg1	SPEV-2					437:442	SPEV-2	437:442	SPEV-2	437:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	94	theme	human	375:379	arg1	lines					404:408	several human and animal cancer cell lines	367:408	several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2	367:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	5	95	from	fractions	975:983	arg1	lectins					933:939	the highly active galactose-specific lectins	896:939	the highly active galactose-specific lectins	896:939	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	5	95	from	fractions	975:983	arg1	present					958:964	present	958:964	present	958:964	The protein and subunit composition of fractions was studied; the highly active galactose-specific lectins were found to be present in these fractions.					
34921890	7	96	theme	gastro-intestinal	1341:1357	arg1	mucosa					1359:1364	gastro-intestinal mucosa	1341:1364	gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1341:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	7	97	dep	system	1288:1293	arg1	activation					1295:1304	activation	1295:1304	activation	1295:1304	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	3	98	theme	SPЕV-2	686:691	arg1	activity					674:681	metabolic activity	664:681	metabolic activity of SPЕV-2, А549 and С6 cell lines	664:715	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	4	99	theme	lines	811:815	arg1	viability					786:794	the viability	782:794	the viability of cancer cell lines	782:815	The effective concentrations of glycoprotein fractions reducing the viability of cancer cell lines were determined.					
34921890	1	100	theme	research	241:248	arg1	area					233:236	a promising area	221:236	a promising area of research in cancer diagnostics and therapies	221:284	Detection and study of biologically active compounds seems a promising area of research in cancer diagnostics and therapies.					
34921890	7	101	theme	effect	1331:1336	arg1	system					1288:1293	immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1281:1434	system	1288:1293	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	7	101	theme	effect	1331:1336	arg1	absence					1314:1320	the absence	1310:1320	the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1310:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	7	102	theme	absence	1314:1320	arg1	indicators					1267:1276	some morphological indicators	1248:1276	some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions	1248:1434	Histological examination revealed some morphological indicators of immune system activation and the absence of toxic effect on gastro-intestinal mucosa of animals at peroral administration of fungal glycoprotein fractions.					
34921890	3	103	theme	lines	711:715	arg1	activity					674:681	metabolic activity	664:681	metabolic activity of SPЕV-2, А549 and С6 cell lines	664:715	It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.					
34921890	2	104	theme	cell	399:402	arg1	HeLa					424:427	HeLa	424:427	HeLa	424:427	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	104	theme	cell	399:402	arg1	Hep-2					417:421	Hep-2	417:421	Hep-2	417:421	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	104	theme	cell	399:402	arg1	A549					411:414	A549	411:414	A549	411:414	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	104	theme	cell	399:402	arg1	SPEV-2					437:442	SPEV-2	437:442	SPEV-2	437:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	2	104	theme	cell	399:402	arg1	lines					404:408	several human and animal cancer cell lines	367:408	several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2	367:442	The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized.					
34921890	1	105	theme	cancer	253:258	arg1	diagnostics					260:270	cancer diagnostics	253:270	cancer diagnostics	253:270	Detection and study of biologically active compounds seems a promising area of research in cancer diagnostics and therapies.					
34044019	4	0	theme	reduced	660:666	arg1	hindrance					675:683	reduced steric hindrance	660:683	reduced steric hindrance between antibody-receptor intermolecular glycan interactions	660:744	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	9	1	theme	rational	1706:1713	arg1	mutagenesis					1715:1725	MD-guided rational mutagenesis	1696:1725	MD-guided rational mutagenesis	1696:1725	Furthermore, MD-guided rational mutagenesis enabled us to demonstrate that Fab-receptor interactions directly contribute to the modulation of antibody-dependent cellular cytotoxicity activity.					
34044019	9	2	theme	cellular	1844:1851	arg1	cytotoxicity					1853:1864	antibody-dependent cellular cytotoxicity	1825:1864	antibody-dependent cellular cytotoxicity activity	1825:1873	Furthermore, MD-guided rational mutagenesis enabled us to demonstrate that Fab-receptor interactions directly contribute to the modulation of antibody-dependent cellular cytotoxicity activity.					
34044019	4	3	theme	receptor	757:764	arg1	affinity					766:773	receptor affinity	757:773	receptor affinity	757:773	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	2	4	theme	mAb	288:290	arg1	biotherapeutics					292:306	novel mAb biotherapeutics	282:306	novel mAb biotherapeutics with tailored biological activities	282:342	Understanding the precise interactions between antibodies and their Fc receptors has led to the engineering of novel mAb biotherapeutics with tailored biological activities.					
34044019	9	5	theme	activity	1866:1873	arg1	modulation					1811:1820	the modulation	1807:1820	the modulation of antibody-dependent cellular cytotoxicity activity	1807:1873	Furthermore, MD-guided rational mutagenesis enabled us to demonstrate that Fab-receptor interactions directly contribute to the modulation of antibody-dependent cellular cytotoxicity activity.					
34044019	6	6	theme	afucosylated	1258:1269	arg1	region					1274:1279	an afucosylated Fc region	1255:1279	an afucosylated Fc region	1255:1279	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	6	7	gly	afucosylated	1258:1269	arg1	region					1274:1279	an afucosylated Fc region	1255:1279	an afucosylated Fc region	1255:1279	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	4	8	theme	glycan	726:731	arg1	interactions					733:744	antibody-receptor intermolecular glycan interactions	693:744	antibody-receptor intermolecular glycan interactions	693:744	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	2	9	theme	biological	322:331	arg1	activities					333:342	tailored biological activities	313:342	tailored biological activities	313:342	Understanding the precise interactions between antibodies and their Fc receptors has led to the engineering of novel mAb biotherapeutics with tailored biological activities.					
34044019	4	10	gly	afucosylation	618:630	arg1	region					642:647	the Fc region	635:647	the Fc region	635:647	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	6	11	theme	FcγRIIIa	1333:1340	arg1	binding					1342:1348	FcγRIIIa binding	1333:1348	FcγRIIIa binding	1333:1348	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	10	12	theme	third	1987:1991	arg1	component					1993:2001	a third component	1985:2001	a third component that influences IgG-Fc receptor biology	1985:2041	This work demonstrates that in addition to Fc-polypeptide and glycan-mediated interactions, the Fab provides a third component that influences IgG-Fc receptor biology.					
34044019	1	13	theme	innate	149:154	arg1	system					163:168	the innate immune system	145:168	the innate immune system	145:168	Binding of antibodies to their receptors is a core component of the innate immune system.					
34044019	6	14	theme	immunoglobulin	1124:1137	arg1	IgG1					1143:1146	IgG1	1143:1146	IgG1	1143:1146	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	6	14	theme	immunoglobulin	1124:1137	arg1	G1					1139:1140	immunoglobulin G1	1124:1140	native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1100:1186	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	8	15	theme	Fc	1601:1602	arg1	fucose					1604:1609	the core Fc fucose	1592:1609	the core Fc fucose in IgG1	1592:1617	The combination of these techniques provides molecular insight into the steric hindrance from the core Fc fucose in IgG1 and corroborates previously proposed Fab-receptor interactions.					
34044019	6	16	theme	fewer	1300:1304	arg1	changes					1321:1327	fewer conformational changes	1300:1327	fewer conformational changes	1300:1327	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	10	17	theme	IgG-Fc	2019:2024	arg1	biology					2035:2041	IgG-Fc receptor biology	2019:2041	IgG-Fc receptor biology	2019:2041	This work demonstrates that in addition to Fc-polypeptide and glycan-mediated interactions, the Fab provides a third component that influences IgG-Fc receptor biology.					
34044019	2	18	with	biotherapeutics	292:306	arg1	activities					333:342	tailored biological activities	313:342	tailored biological activities	313:342	Understanding the precise interactions between antibodies and their Fc receptors has led to the engineering of novel mAb biotherapeutics with tailored biological activities.					
34044019	4	19	theme	structure	568:576	arg1	analysis					578:585	Crystal structure analysis	560:585	Crystal structure analysis	560:585	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	1	20	theme	antibodies	92:101	arg1	component					132:140	a core component	125:140	a core component of the innate immune system	125:168	Binding of antibodies to their receptors is a core component of the innate immune system.					
34044019	1	20	theme	antibodies	92:101	arg1	Binding					81:87	Binding	81:87	Binding of antibodies to their receptors	81:120	Binding of antibodies to their receptors is a core component of the innate immune system.					
34044019	6	21	theme	solvent	1070:1076	arg1	accessibility					1078:1090	solvent accessibility	1070:1090	solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1070:1186	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	3	22	theme	findings	373:380	arg1	findings					373:380	the most significant findings	352:380	the most significant findings	352:380	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	3	22	theme	findings	373:380	arg1	One					345:347	One	345:347	One	345:347	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	9	23	theme	MD-guided	1696:1704	arg1	mutagenesis					1715:1725	MD-guided rational mutagenesis	1696:1725	MD-guided rational mutagenesis	1696:1725	Furthermore, MD-guided rational mutagenesis enabled us to demonstrate that Fab-receptor interactions directly contribute to the modulation of antibody-dependent cellular cytotoxicity activity.					
34044019	7	24	theme	molecular	1441:1449	arg1	mechanism					1451:1459	the molecular mechanism	1437:1459	the molecular mechanism behind the effects of afucosylation	1437:1495	In addition, we performed extensive molecular dynamics (MD) simulations to understand the molecular mechanism behind the effects of afucosylation.					
34044019	8	25	theme	Fab-receptor	1656:1667	arg1	interactions					1669:1680	previously proposed Fab-receptor interactions	1636:1680	previously proposed Fab-receptor interactions	1636:1680	The combination of these techniques provides molecular insight into the steric hindrance from the core Fc fucose in IgG1 and corroborates previously proposed Fab-receptor interactions.					
34044019	0	26	theme	Antibody-receptor	0:16	arg1	interactions					18:29	Antibody-receptor interactions	0:29	Antibody-receptor interactions	0:29	Antibody-receptor interactions mediate antibody-dependent cellular cytotoxicity.					
34044019	6	27	theme	hydroxyl	1155:1162	arg1	radical					1164:1170	hydroxyl radical	1155:1170	hydroxyl radical	1155:1170	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	3	28	theme	monoclonal	403:412	arg1	antibodies					414:423	afucosylated monoclonal antibodies	390:423	afucosylated monoclonal antibodies	390:423	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	4	29	from	afucosylation	618:630	arg1	region					642:647	the Fc region	635:647	the Fc region	635:647	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	10	30	theme	glycan-mediated	1938:1952	arg1	interactions					1954:1965	glycan-mediated interactions	1938:1965	glycan-mediated interactions	1938:1965	This work demonstrates that in addition to Fc-polypeptide and glycan-mediated interactions, the Fab provides a third component that influences IgG-Fc receptor biology.					
34044019	1	31	theme	core	127:130	arg1	component					132:140	a core component	125:140	a core component of the innate immune system	125:168	Binding of antibodies to their receptors is a core component of the innate immune system.					
34044019	1	31	theme	core	127:130	arg1	Binding					81:87	Binding	81:87	Binding of antibodies to their receptors	81:120	Binding of antibodies to their receptors is a core component of the innate immune system.					
34044019	3	32	theme	commensurate	493:504	arg1	increase					506:513	a commensurate increase	491:513	a commensurate increase in antibody-dependent cellular cytotoxicity	491:557	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	0	33	theme	cellular	58:65	arg1	cytotoxicity					67:78	antibody-dependent cellular cytotoxicity	39:78	antibody-dependent cellular cytotoxicity	39:78	Antibody-receptor interactions mediate antibody-dependent cellular cytotoxicity.					
34044019	7	34	theme	MD	1407:1408	arg1	simulations					1411:1421	extensive molecular dynamics (MD) simulations	1377:1421	extensive molecular dynamics (MD) simulations	1377:1421	In addition, we performed extensive molecular dynamics (MD) simulations to understand the molecular mechanism behind the effects of afucosylation.					
34044019	8	35	theme	techniques	1523:1532	arg1	combination					1502:1512	The combination	1498:1512	The combination of these techniques	1498:1532	The combination of these techniques provides molecular insight into the steric hindrance from the core Fc fucose in IgG1 and corroborates previously proposed Fab-receptor interactions.					
34044019	3	36	from	increase	506:513	arg1	cytotoxicity					546:557	antibody-dependent cellular cytotoxicity	518:557	antibody-dependent cellular cytotoxicity	518:557	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	9	37	theme	Fab-receptor	1758:1769	arg1	interactions					1771:1782	Fab-receptor interactions	1758:1782	Fab-receptor interactions	1758:1782	Furthermore, MD-guided rational mutagenesis enabled us to demonstrate that Fab-receptor interactions directly contribute to the modulation of antibody-dependent cellular cytotoxicity activity.					
34044019	7	38	theme	dynamics	1397:1404	arg1	simulations					1411:1421	extensive molecular dynamics (MD) simulations	1377:1421	extensive molecular dynamics (MD) simulations	1377:1421	In addition, we performed extensive molecular dynamics (MD) simulations to understand the molecular mechanism behind the effects of afucosylation.					
34044019	8	39	theme	molecular	1543:1551	arg1	insight					1553:1559	molecular insight	1543:1559	molecular insight into the steric hindrance from the core Fc fucose in IgG1	1543:1617	The combination of these techniques provides molecular insight into the steric hindrance from the core Fc fucose in IgG1 and corroborates previously proposed Fab-receptor interactions.					
34044019	3	40	theme	cellular	537:544	arg1	cytotoxicity					546:557	antibody-dependent cellular cytotoxicity	518:557	antibody-dependent cellular cytotoxicity	518:557	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	5	41	theme	Fab	916:918	arg1	region					921:926	the fragment antigen-binding (Fab) region	886:926	the fragment antigen-binding (Fab) region	886:926	In addition, recent work has shown that the fragment antigen-binding (Fab) region may directly interact with Fc receptors; however, the biological consequences of these interactions remain unclear.					
34044019	6	42	theme	solution-phase	1210:1223	arg1	evidence					1225:1232	the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding	1200:1348	the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding	1200:1348	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	4	43	theme	steric	668:673	arg1	hindrance					675:683	reduced steric hindrance	660:683	reduced steric hindrance between antibody-receptor intermolecular glycan interactions	660:744	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	6	44	gly	afucosylated	1111:1122	arg1	footprinting-MS					1172:1186	native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1100:1186	native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1100:1186	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	2	45	theme	tailored	313:320	arg1	activities					333:342	tailored biological activities	313:342	tailored biological activities	313:342	Understanding the precise interactions between antibodies and their Fc receptors has led to the engineering of novel mAb biotherapeutics with tailored biological activities.					
34044019	9	46	theme	antibody-dependent	1825:1842	arg1	cytotoxicity					1853:1864	antibody-dependent cellular cytotoxicity	1825:1864	antibody-dependent cellular cytotoxicity activity	1825:1873	Furthermore, MD-guided rational mutagenesis enabled us to demonstrate that Fab-receptor interactions directly contribute to the modulation of antibody-dependent cellular cytotoxicity activity.					
34044019	2	47	theme	biotherapeutics	292:306	arg1	engineering					267:277	the engineering	263:277	the engineering of novel mAb biotherapeutics with tailored biological activities	263:342	Understanding the precise interactions between antibodies and their Fc receptors has led to the engineering of novel mAb biotherapeutics with tailored biological activities.					
34044019	9	48	theme	cytotoxicity	1853:1864	arg1	activity					1866:1873	antibody-dependent cellular cytotoxicity activity	1825:1873	antibody-dependent cellular cytotoxicity activity	1825:1873	Furthermore, MD-guided rational mutagenesis enabled us to demonstrate that Fab-receptor interactions directly contribute to the modulation of antibody-dependent cellular cytotoxicity activity.					
34044019	6	49	theme	Fc	1271:1272	arg1	region					1274:1279	an afucosylated Fc region	1255:1279	an afucosylated Fc region	1255:1279	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	5	50	theme	Fc	955:956	arg1	receptors					958:966	Fc receptors	955:966	Fc receptors	955:966	In addition, recent work has shown that the fragment antigen-binding (Fab) region may directly interact with Fc receptors; however, the biological consequences of these interactions remain unclear.					
34044019	7	51	dep	mechanism	1451:1459	arg1	behind					1461:1466	behind	1461:1466	behind the effects of afucosylation	1461:1495	In addition, we performed extensive molecular dynamics (MD) simulations to understand the molecular mechanism behind the effects of afucosylation.					
34044019	8	52	theme	steric	1570:1575	arg1	hindrance					1577:1585	the steric hindrance	1566:1585	the steric hindrance from the core Fc fucose in IgG1	1566:1617	The combination of these techniques provides molecular insight into the steric hindrance from the core Fc fucose in IgG1 and corroborates previously proposed Fab-receptor interactions.					
34044019	4	53	theme	intermolecular	711:724	arg1	interactions					733:744	antibody-receptor intermolecular glycan interactions	693:744	antibody-receptor intermolecular glycan interactions	693:744	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	5	54	theme	biological	982:991	arg1	consequences					993:1004	the biological consequences	978:1004	the biological consequences of these interactions	978:1026	In addition, recent work has shown that the fragment antigen-binding (Fab) region may directly interact with Fc receptors; however, the biological consequences of these interactions remain unclear.					
34044019	8	55	theme	core	1596:1599	arg1	fucose					1604:1609	the core Fc fucose	1592:1609	the core Fc fucose in IgG1	1592:1617	The combination of these techniques provides molecular insight into the steric hindrance from the core Fc fucose in IgG1 and corroborates previously proposed Fab-receptor interactions.					
34044019	7	56	theme	afucosylation	1483:1495	arg1	effects					1472:1478	the effects	1468:1478	the effects of afucosylation	1468:1495	In addition, we performed extensive molecular dynamics (MD) simulations to understand the molecular mechanism behind the effects of afucosylation.					
34044019	2	57	theme	novel	282:286	arg1	biotherapeutics					292:306	novel mAb biotherapeutics	282:306	novel mAb biotherapeutics with tailored biological activities	282:342	Understanding the precise interactions between antibodies and their Fc receptors has led to the engineering of novel mAb biotherapeutics with tailored biological activities.					
34044019	6	58	theme	G1	1139:1140	arg1	footprinting-MS					1172:1186	native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1100:1186	native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1100:1186	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	6	59	theme	conformational	1306:1319	arg1	changes					1321:1327	fewer conformational changes	1300:1327	fewer conformational changes	1300:1327	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	10	60	theme	receptor	2026:2033	arg1	biology					2035:2041	IgG-Fc receptor biology	2019:2041	IgG-Fc receptor biology	2019:2041	This work demonstrates that in addition to Fc-polypeptide and glycan-mediated interactions, the Fab provides a third component that influences IgG-Fc receptor biology.					
34044019	1	61	theme	immune	156:161	arg1	system					163:168	the innate immune system	145:168	the innate immune system	145:168	Binding of antibodies to their receptors is a core component of the innate immune system.					
34044019	6	62	theme	afucosylated	1111:1122	arg1	footprinting-MS					1172:1186	native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1100:1186	native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1100:1186	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	3	63	theme	significant	361:371	arg1	findings					373:380	the most significant findings	352:380	the most significant findings	352:380	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	5	64	theme	recent	859:864	arg1	work					866:869	recent work	859:869	recent work	859:869	In addition, recent work has shown that the fragment antigen-binding (Fab) region may directly interact with Fc receptors; however, the biological consequences of these interactions remain unclear.					
34044019	6	65	theme	native	1100:1105	arg1	footprinting-MS					1172:1186	native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1100:1186	native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1100:1186	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	4	66	theme	solution-phase	785:798	arg1	data					800:803	solution-phase data	785:803	solution-phase data	785:803	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	8	67	from	fucose	1604:1609	arg1	hindrance					1577:1585	the steric hindrance	1566:1585	the steric hindrance from the core Fc fucose in IgG1	1566:1617	The combination of these techniques provides molecular insight into the steric hindrance from the core Fc fucose in IgG1 and corroborates previously proposed Fab-receptor interactions.					
34044019	8	67	from	fucose	1604:1609	arg1	IgG1					1614:1617	IgG1	1614:1617	IgG1	1614:1617	The combination of these techniques provides molecular insight into the steric hindrance from the core Fc fucose in IgG1 and corroborates previously proposed Fab-receptor interactions.					
34044019	3	68	theme	afucosylated	390:401	arg1	antibodies					414:423	afucosylated monoclonal antibodies	390:423	afucosylated monoclonal antibodies	390:423	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	8	69	theme	proposed	1647:1654	arg1	interactions					1669:1680	previously proposed Fab-receptor interactions	1636:1680	previously proposed Fab-receptor interactions	1636:1680	The combination of these techniques provides molecular insight into the steric hindrance from the core Fc fucose in IgG1 and corroborates previously proposed Fab-receptor interactions.					
34044019	7	70	theme	extensive	1377:1385	arg1	simulations					1411:1421	extensive molecular dynamics (MD) simulations	1377:1421	extensive molecular dynamics (MD) simulations	1377:1421	In addition, we performed extensive molecular dynamics (MD) simulations to understand the molecular mechanism behind the effects of afucosylation.					
34044019	4	71	theme	Crystal	560:566	arg1	analysis					578:585	Crystal structure analysis	560:585	Crystal structure analysis	560:585	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	5	72	theme	fragment	890:897	arg1	region					921:926	the fragment antigen-binding (Fab) region	886:926	the fragment antigen-binding (Fab) region	886:926	In addition, recent work has shown that the fragment antigen-binding (Fab) region may directly interact with Fc receptors; however, the biological consequences of these interactions remain unclear.					
34044019	5	73	theme	interactions	1015:1026	arg1	consequences					993:1004	the biological consequences	978:1004	the biological consequences of these interactions	978:1026	In addition, recent work has shown that the fragment antigen-binding (Fab) region may directly interact with Fc receptors; however, the biological consequences of these interactions remain unclear.					
34044019	3	74	theme	increased	437:445	arg1	affinity					447:454	increased affinity	437:454	increased affinity toward the receptor FcγRIIIa	437:483	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	0	75	theme	antibody-dependent	39:56	arg1	cytotoxicity					67:78	antibody-dependent cellular cytotoxicity	39:78	antibody-dependent cellular cytotoxicity	39:78	Antibody-receptor interactions mediate antibody-dependent cellular cytotoxicity.					
34044019	5	76	theme	antigen-binding	899:913	arg1	region					921:926	the fragment antigen-binding (Fab) region	886:926	the fragment antigen-binding (Fab) region	886:926	In addition, recent work has shown that the fragment antigen-binding (Fab) region may directly interact with Fc receptors; however, the biological consequences of these interactions remain unclear.					
34044019	3	77	gly	afucosylated	390:401	arg1	antibodies					414:423	afucosylated monoclonal antibodies	390:423	afucosylated monoclonal antibodies	390:423	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	4	78	theme	Fc	639:640	arg1	region					642:647	the Fc region	635:647	the Fc region	635:647	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34044019	3	79	theme	antibody-dependent	518:535	arg1	cytotoxicity					546:557	antibody-dependent cellular cytotoxicity	518:557	antibody-dependent cellular cytotoxicity	518:557	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	3	80	theme	receptor	467:474	arg1	FcγRIIIa					476:483	the receptor FcγRIIIa	463:483	the receptor FcγRIIIa	463:483	One of the most significant findings is that afucosylated monoclonal antibodies demonstrate increased affinity toward the receptor FcγRIIIa, with a commensurate increase in antibody-dependent cellular cytotoxicity.					
34044019	6	81	from	differences	1055:1065	arg1	accessibility					1078:1090	solvent accessibility	1070:1090	solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS	1070:1186	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	2	82	theme	precise	189:195	arg1	interactions					197:208	the precise interactions	185:208	the precise interactions between antibodies and their Fc receptors	185:250	Understanding the precise interactions between antibodies and their Fc receptors has led to the engineering of novel mAb biotherapeutics with tailored biological activities.					
34044019	7	83	theme	molecular	1387:1395	arg1	simulations					1411:1421	extensive molecular dynamics (MD) simulations	1377:1421	extensive molecular dynamics (MD) simulations	1377:1421	In addition, we performed extensive molecular dynamics (MD) simulations to understand the molecular mechanism behind the effects of afucosylation.					
34044019	2	84	theme	Fc	239:240	arg1	receptors					242:250	their Fc receptors	233:250	their Fc receptors	233:250	Understanding the precise interactions between antibodies and their Fc receptors has led to the engineering of novel mAb biotherapeutics with tailored biological activities.					
34044019	1	85	theme	system	163:168	arg1	component					132:140	a core component	125:140	a core component of the innate immune system	125:168	Binding of antibodies to their receptors is a core component of the innate immune system.					
34044019	1	85	theme	system	163:168	arg1	Binding					81:87	Binding	81:87	Binding of antibodies to their receptors	81:120	Binding of antibodies to their receptors is a core component of the innate immune system.					
34044019	6	86	theme	first	1204:1208	arg1	evidence					1225:1232	the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding	1200:1348	the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding	1200:1348	By probing differences in solvent accessibility between native and afucosylated immunoglobulin G1 (IgG1) using hydroxyl radical footprinting-MS, we provide the first solution-phase evidence that an IgG1 bearing an afucosylated Fc region appears to require fewer conformational changes for FcγRIIIa binding.					
34044019	4	87	theme	antibody-receptor	693:709	arg1	interactions					733:744	antibody-receptor intermolecular glycan interactions	693:744	antibody-receptor intermolecular glycan interactions	693:744	Crystal structure analysis has led to the hypothesis that afucosylation in the Fc region results in reduced steric hindrance between antibody-receptor intermolecular glycan interactions, enhancing receptor affinity; however, solution-phase data have yet to corroborate this hypothesis.					
34583992	0	0	theme	lectins	92:98	arg1	binding					52:58	binding	52:58	binding of soluble and virus-associated lectins to host cell glycan receptors	52:128	Glycocalyx crowding with mucin mimetics strengthens binding of soluble and virus-associated lectins to host cell glycan receptors.					
34583992	3	1	theme	blood	727:731	arg1	RBCs					740:743	RBCs	740:743	RBCs	740:743	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	3	1	theme	blood	727:731	arg1	cells					733:737	red blood cells	723:737	red blood cells (RBCs)	723:744	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	6	2	theme	techniques	1267:1276	arg1	range					1234:1238	a range	1232:1238	a range of biophysical and imaging techniques	1232:1276	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	6	3	from	enhancement	1365:1375	arg1	clustering					1387:1396	lectin clustering	1380:1396	lectin clustering	1380:1396	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	1	4	theme	cell	177:180	arg1	surfaces					182:189	epithelial cell surfaces	166:189	epithelial cell surfaces	166:189	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	2	5	from	composition	583:593	arg1	cells					605:609	living cells	598:609	living cells	598:609	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	0	6	theme	virus-associated	75:90	arg1	lectins					92:98	soluble and virus-associated lectins	63:98	soluble and virus-associated lectins	63:98	Glycocalyx crowding with mucin mimetics strengthens binding of soluble and virus-associated lectins to host cell glycan receptors.					
34583992	2	7	theme	molecular	616:624	arg1	precision					626:634	molecular precision	616:634	molecular precision	616:634	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	6	8	theme	concomitant	1353:1363	arg1	enhancement					1365:1375	concomitant enhancement	1353:1375	concomitant enhancement in lectin clustering	1353:1396	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	6	9	theme	patch	1468:1472	arg1	formation					1422:1430	the formation	1418:1430	the formation of a more extensive glycan receptor patch at the cell membrane	1418:1493	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	0	10	theme	host	103:106	arg1	receptors					120:128	host cell glycan receptors	103:128	host cell glycan receptors	103:128	Glycocalyx crowding with mucin mimetics strengthens binding of soluble and virus-associated lectins to host cell glycan receptors.					
34583992	6	11	theme	SNA	1194:1196	arg1	behavior					1198:1205	SNA behavior	1194:1205	SNA behavior	1194:1205	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	6	12	theme	behavior	1198:1205	arg1	characterization					1174:1189	Careful characterization	1166:1189	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques	1166:1276	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	6	13	theme	receptor	1459:1466	arg1	patch					1468:1472	a more extensive glycan receptor patch	1435:1472	a more extensive glycan receptor patch at the cell membrane	1435:1493	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	7	14	theme	biophysical	1566:1576	arg1	properties					1596:1605	the biophysical and biomechanical properties	1562:1605	the biophysical and biomechanical properties of mucins	1562:1615	Our findings indicate that glycan-targeting pathogens may exploit the biophysical and biomechanical properties of mucins to overcome the mucosal glycocalyx barrier.					
34583992	2	15	theme	tools	533:537	arg1	tools					533:537	tools	533:537	tools available for tailoring their structure and composition in living cells with molecular precision	533:634	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	2	15	theme	tools	533:537	arg1	repertoire					519:528	a limited repertoire	509:528	a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision	509:634	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	6	16	theme	biophysical	1243:1253	arg1	techniques					1267:1276	biophysical and imaging techniques	1243:1276	techniques	1267:1276	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	6	17	theme	glycocalyx	1337:1346	arg1	crowding					1302:1309	lectin-induced crowding	1287:1309	lectin-induced crowding	1287:1309	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	6	17	theme	glycocalyx	1337:1346	arg1	reorganization					1315:1328	reorganization	1315:1328	reorganization	1315:1328	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	0	18	theme	glycan	113:118	arg1	receptors					120:128	host cell glycan receptors	103:128	host cell glycan receptors	103:128	Glycocalyx crowding with mucin mimetics strengthens binding of soluble and virus-associated lectins to host cell glycan receptors.					
34583992	6	19	theme	lectin-induced	1287:1300	arg1	crowding					1302:1309	lectin-induced crowding	1287:1309	lectin-induced crowding	1287:1309	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	3	20	theme	endogenous	819:828	arg1	receptors					842:850	endogenous sialic acid receptors	819:850	endogenous sialic acid receptors	819:850	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	0	21	theme	cell	108:111	arg1	receptors					120:128	host cell glycan receptors	103:128	host cell glycan receptors	103:128	Glycocalyx crowding with mucin mimetics strengthens binding of soluble and virus-associated lectins to host cell glycan receptors.					
34583992	1	22	theme	target	387:392	arg1	receptors					394:402	target receptors	387:402	target receptors on host cells	387:416	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	4	23	theme	current	973:979	arg1	view					981:984	the current view	969:984	the current view of mucins as providing a protective shield against pathogens	969:1045	The glycocalyx inhibited the rate of SNA and H1N1 adhesion in a size- and density-dependent manner, consistent with the current view of mucins as providing a protective shield against pathogens.					
34583992	7	24	theme	biomechanical	1582:1594	arg1	properties					1596:1605	the biophysical and biomechanical properties	1562:1605	the biophysical and biomechanical properties of mucins	1562:1615	Our findings indicate that glycan-targeting pathogens may exploit the biophysical and biomechanical properties of mucins to overcome the mucosal glycocalyx barrier.					
34583992	1	25	from	receptors	394:402	arg1	cells					412:416	host cells	407:416	host cells	407:416	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	1	26	theme	pathogenic	199:208	arg1	threats					210:216	pathogenic threats	199:216	pathogenic threats	199:216	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	6	27	theme	glycan	1452:1457	arg1	patch					1468:1472	a more extensive glycan receptor patch	1435:1472	a more extensive glycan receptor patch at the cell membrane	1435:1493	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	6	28	theme	lectin	1380:1385	arg1	clustering					1387:1396	lectin clustering	1380:1396	lectin clustering	1380:1396	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	4	29	theme	size-	917:921	arg1	manner					945:950	a size- and density-dependent manner	915:950	a size- and density-dependent manner	915:950	The glycocalyx inhibited the rate of SNA and H1N1 adhesion in a size- and density-dependent manner, consistent with the current view of mucins as providing a protective shield against pathogens.					
34583992	0	30	theme	Glycocalyx	0:9	arg1	crowding					11:18	Glycocalyx crowding	0:18	Glycocalyx crowding with mucin mimetics	0:38	Glycocalyx crowding with mucin mimetics strengthens binding of soluble and virus-associated lectins to host cell glycan receptors.					
34583992	3	31	from	influence	764:772	arg1	lectin					777:782	lectin	777:782	lectin (SNA)	777:788	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	3	31	from	influence	764:772	arg1	SNA					785:787	SNA	785:787	SNA	785:787	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	7	32	theme	mucosal	1633:1639	arg1	barrier					1652:1658	the mucosal glycocalyx barrier	1629:1658	the mucosal glycocalyx barrier	1629:1658	Our findings indicate that glycan-targeting pathogens may exploit the biophysical and biomechanical properties of mucins to overcome the mucosal glycocalyx barrier.					
34583992	4	33	theme	mucins	989:994	arg1	view					981:984	the current view	969:984	the current view of mucins as providing a protective shield against pathogens	969:1045	The glycocalyx inhibited the rate of SNA and H1N1 adhesion in a size- and density-dependent manner, consistent with the current view of mucins as providing a protective shield against pathogens.					
34583992	6	34	theme	imaging	1259:1265	arg1	techniques					1267:1276	biophysical and imaging techniques	1243:1276	techniques	1267:1276	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	0	35	theme	mucin	25:29	arg1	mimetics					31:38	mucin mimetics	25:38	mucin mimetics	25:38	Glycocalyx crowding with mucin mimetics strengthens binding of soluble and virus-associated lectins to host cell glycan receptors.					
34583992	1	36	theme	cell	308:311	arg1	surface					313:319	the cell surface	304:319	the cell surface	304:319	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	4	37	theme	H1N1	898:901	arg1	adhesion					903:910	H1N1 adhesion	898:910	H1N1 adhesion	898:910	The glycocalyx inhibited the rate of SNA and H1N1 adhesion in a size- and density-dependent manner, consistent with the current view of mucins as providing a protective shield against pathogens.					
34583992	1	38	dep	protect	158:164	arg1	erecting					328:335	erecting	328:335	erecting a physical barrier that restricts their access to target receptors on host cells	328:416	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	1	38	dep	protect	158:164	arg1	serving					221:227	serving	221:227	serving as nonproductive decoys that capture infectious agents and clear them from the cell surface	221:319	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	5	39	theme	mimetics	1104:1111	arg1	density					1083:1089	the density	1079:1089	the density of the mucin mimetics	1079:1111	Counterintuitively, increasing the density of the mucin mimetics enhanced the retention of bound lectins and viruses.					
34583992	3	40	theme	mucins	678:683	arg1	mimetics					666:673	synthetic glycopolymer mimetics	643:673	synthetic glycopolymer mimetics of mucins	643:683	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	6	41	with	crowding	1302:1309	arg1	enhancement					1365:1375	concomitant enhancement	1353:1375	concomitant enhancement in lectin clustering	1353:1396	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	3	42	from	glycocalyx	709:718	arg1	RBCs					740:743	RBCs	740:743	RBCs	740:743	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	3	42	from	glycocalyx	709:718	arg1	cells					733:737	red blood cells	723:737	red blood cells (RBCs)	723:744	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	6	43	with	reorganization	1315:1328	arg1	enhancement					1365:1375	concomitant enhancement	1353:1375	concomitant enhancement in lectin clustering	1353:1396	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	3	44	from	adhesion	807:814	arg1	lectin					777:782	lectin	777:782	lectin (SNA)	777:788	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	3	44	from	adhesion	807:814	arg1	SNA					785:787	SNA	785:787	SNA	785:787	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	1	45	theme	host	407:410	arg1	cells					412:416	host cells	407:416	host cells	407:416	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	6	46	from	membrane	1486:1493	arg1	formation					1422:1430	the formation	1418:1430	the formation of a more extensive glycan receptor patch at the cell membrane	1418:1493	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	6	46	from	membrane	1486:1493	arg1	patch					1468:1472	a more extensive glycan receptor patch	1435:1472	a more extensive glycan receptor patch at the cell membrane	1435:1493	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	6	47	theme	cell	1481:1484	arg1	membrane					1486:1493	the cell membrane	1477:1493	the cell membrane	1477:1493	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	1	48	theme	nonproductive	232:244	arg1	barrier					348:354	a physical barrier	337:354	a physical barrier that restricts their access to target receptors on host cells	337:416	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	1	48	theme	nonproductive	232:244	arg1	decoys					246:251	nonproductive decoys	232:251	nonproductive decoys that capture infectious agents and clear them from the cell surface	232:319	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	7	49	theme	glycocalyx	1641:1650	arg1	barrier					1652:1658	the mucosal glycocalyx barrier	1629:1658	the mucosal glycocalyx barrier	1629:1658	Our findings indicate that glycan-targeting pathogens may exploit the biophysical and biomechanical properties of mucins to overcome the mucosal glycocalyx barrier.					
34583992	4	50	theme	adhesion	903:910	arg1	rate					882:885	the rate	878:885	the rate of SNA and H1N1 adhesion	878:910	The glycocalyx inhibited the rate of SNA and H1N1 adhesion in a size- and density-dependent manner, consistent with the current view of mucins as providing a protective shield against pathogens.					
34583992	2	51	from	structure	569:577	arg1	cells					605:609	living cells	598:609	living cells	598:609	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	5	52	theme	lectins	1145:1151	arg1	retention					1126:1134	the retention	1122:1134	the retention of bound lectins and viruses	1122:1163	Counterintuitively, increasing the density of the mucin mimetics enhanced the retention of bound lectins and viruses.					
34583992	2	53	theme	available	539:547	arg1	tools					533:537	tools	533:537	tools available for tailoring their structure and composition in living cells with molecular precision	533:634	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	2	54	theme	living	598:603	arg1	cells					605:609	living cells	598:609	living cells	598:609	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	5	55	theme	viruses	1157:1163	arg1	retention					1126:1134	the retention	1122:1134	the retention of bound lectins and viruses	1122:1163	Counterintuitively, increasing the density of the mucin mimetics enhanced the retention of bound lectins and viruses.					
34583992	4	56	with	consistent	953:962	arg1	view					981:984	the current view	969:984	the current view of mucins as providing a protective shield against pathogens	969:1045	The glycocalyx inhibited the rate of SNA and H1N1 adhesion in a size- and density-dependent manner, consistent with the current view of mucins as providing a protective shield against pathogens.					
34583992	6	57	theme	extensive	1442:1450	arg1	patch					1468:1472	a more extensive glycan receptor patch	1435:1472	a more extensive glycan receptor patch at the cell membrane	1435:1493	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	5	58	theme	bound	1139:1143	arg1	lectins					1145:1151	bound lectins	1139:1151	bound lectins	1139:1151	Counterintuitively, increasing the density of the mucin mimetics enhanced the retention of bound lectins and viruses.					
34583992	5	59	theme	mucin	1098:1102	arg1	mimetics					1104:1111	the mucin mimetics	1094:1111	the mucin mimetics	1094:1111	Counterintuitively, increasing the density of the mucin mimetics enhanced the retention of bound lectins and viruses.					
34583992	7	60	theme	glycan-targeting	1523:1538	arg1	pathogens					1540:1548	glycan-targeting pathogens	1523:1548	glycan-targeting pathogens	1523:1548	Our findings indicate that glycan-targeting pathogens may exploit the biophysical and biomechanical properties of mucins to overcome the mucosal glycocalyx barrier.					
34583992	0	61	with	crowding	11:18	arg1	mimetics					31:38	mucin mimetics	25:38	mucin mimetics	25:38	Glycocalyx crowding with mucin mimetics strengthens binding of soluble and virus-associated lectins to host cell glycan receptors.					
34583992	6	62	from	surface	1218:1224	arg1	characterization					1174:1189	Careful characterization	1166:1189	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques	1166:1276	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	3	63	theme	acid	837:840	arg1	receptors					842:850	endogenous sialic acid receptors	819:850	endogenous sialic acid receptors	819:850	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	1	64	theme	physical	339:346	arg1	barrier					348:354	a physical barrier	337:354	a physical barrier that restricts their access to target receptors on host cells	337:416	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	1	64	theme	physical	339:346	arg1	decoys					246:251	nonproductive decoys	232:251	nonproductive decoys that capture infectious agents and clear them from the cell surface	232:319	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	4	65	theme	SNA	890:892	arg1	rate					882:885	the rate	878:885	the rate of SNA and H1N1 adhesion	878:910	The glycocalyx inhibited the rate of SNA and H1N1 adhesion in a size- and density-dependent manner, consistent with the current view of mucins as providing a protective shield against pathogens.					
34583992	3	66	theme	mucosal	701:707	arg1	glycocalyx					709:718	the mucosal glycocalyx	697:718	the mucosal glycocalyx on red blood cells (RBCs)	697:744	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	3	67	theme	synthetic	643:651	arg1	mimetics					666:673	synthetic glycopolymer mimetics	643:673	synthetic glycopolymer mimetics of mucins	643:683	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	6	68	theme	RBC	1214:1216	arg1	surface					1218:1224	the RBC surface	1210:1224	the RBC surface using a range of biophysical and imaging techniques	1210:1276	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	1	69	theme	Membrane-associated	131:149	arg1	mucins					151:156	Membrane-associated mucins	131:156	Membrane-associated mucins	131:156	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	3	70	theme	virus	794:798	arg1	adhesion					807:814	virus (H1N1) adhesion	794:814	virus (H1N1) adhesion to endogenous sialic acid receptors	794:850	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	3	71	theme	glycopolymer	653:664	arg1	mimetics					666:673	synthetic glycopolymer mimetics	643:673	synthetic glycopolymer mimetics of mucins	643:683	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	2	72	with	structure	569:577	arg1	precision					626:634	molecular precision	616:634	molecular precision	616:634	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	2	73	theme	limited	511:517	arg1	tools					533:537	tools	533:537	tools available for tailoring their structure and composition in living cells with molecular precision	533:634	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	2	73	theme	limited	511:517	arg1	repertoire					519:528	a limited repertoire	509:528	a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision	509:634	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	6	74	from	formation	1422:1430	arg1	membrane					1486:1493	the cell membrane	1477:1493	the cell membrane	1477:1493	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	3	75	theme	sialic	830:835	arg1	receptors					842:850	endogenous sialic acid receptors	819:850	endogenous sialic acid receptors	819:850	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	1	76	theme	infectious	266:275	arg1	agents					277:282	infectious agents	266:282	infectious agents	266:282	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	0	77	theme	soluble	63:69	arg1	lectins					92:98	soluble and virus-associated lectins	63:98	soluble and virus-associated lectins	63:98	Glycocalyx crowding with mucin mimetics strengthens binding of soluble and virus-associated lectins to host cell glycan receptors.					
34583992	3	78	theme	red	723:725	arg1	RBCs					740:743	RBCs	740:743	RBCs	740:743	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	3	78	theme	red	723:725	arg1	cells					733:737	red blood cells	723:737	red blood cells (RBCs)	723:744	Using synthetic glycopolymer mimetics of mucins, we modeled the mucosal glycocalyx on red blood cells (RBCs) and evaluated its influence on lectin (SNA) and virus (H1N1) adhesion to endogenous sialic acid receptors.					
34583992	7	79	theme	mucins	1610:1615	arg1	properties					1596:1605	the biophysical and biomechanical properties	1562:1605	the biophysical and biomechanical properties of mucins	1562:1615	Our findings indicate that glycan-targeting pathogens may exploit the biophysical and biomechanical properties of mucins to overcome the mucosal glycocalyx barrier.					
34583992	6	80	theme	Careful	1166:1172	arg1	characterization					1174:1189	Careful characterization	1166:1189	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques	1166:1276	Careful characterization of SNA behavior at the RBC surface using a range of biophysical and imaging techniques revealed lectin-induced crowding and reorganization of the glycocalyx with concomitant enhancement in lectin clustering, presumably through the formation of a more extensive glycan receptor patch at the cell membrane.					
34583992	1	81	theme	epithelial	166:175	arg1	surfaces					182:189	epithelial cell surfaces	166:189	epithelial cell surfaces	166:189	Membrane-associated mucins protect epithelial cell surfaces against pathogenic threats by serving as nonproductive decoys that capture infectious agents and clear them from the cell surface and by erecting a physical barrier that restricts their access to target receptors on host cells.					
34583992	2	82	with	composition	583:593	arg1	precision					626:634	molecular precision	616:634	molecular precision	616:634	However, the mechanisms through which mucins function are still poorly defined because of a limited repertoire of tools available for tailoring their structure and composition in living cells with molecular precision.					
34583992	4	83	theme	density-dependent	927:943	arg1	manner					945:950	a size- and density-dependent manner	915:950	a size- and density-dependent manner	915:950	The glycocalyx inhibited the rate of SNA and H1N1 adhesion in a size- and density-dependent manner, consistent with the current view of mucins as providing a protective shield against pathogens.					
34000071	0	0	from	adsorption	26:35	arg1	cellulose-chitosan					62:79	cellulose-chitosan	62:79	cellulose-chitosan	62:79	Environmentally dependent adsorption of 2,4-dichlorophenol on cellulose-chitosan self-assembled composites.					
34000071	4	1	from	structure	720:728	arg1	successful					693:702	successful	693:702	successful	693:702	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	4	1	from	structure	720:728	arg1	adsorption					678:687	The adsorption	674:687	The adsorption	674:687	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	5	2	theme	hydrogel	1147:1154	arg1	structures					1156:1165	the never dried hydrogel structures	1131:1165	the never dried hydrogel structures	1131:1165	Finally, adsorbent regeneration was successfully tested for both, dry (rewetted) and never-dried states, showing improved adsorption after regeneration in the case of the never dried hydrogel structures.					
34000071	5	3	theme	structures	1156:1165	arg1	case					1123:1126	the case	1119:1126	the case of the never dried hydrogel structures	1119:1165	Finally, adsorbent regeneration was successfully tested for both, dry (rewetted) and never-dried states, showing improved adsorption after regeneration in the case of the never dried hydrogel structures.					
34000071	5	4	theme	dry	1030:1032	arg1	states					1061:1066	both, dry (rewetted) and never-dried states	1024:1066	states	1061:1066	Finally, adsorbent regeneration was successfully tested for both, dry (rewetted) and never-dried states, showing improved adsorption after regeneration in the case of the never dried hydrogel structures.					
34000071	5	5	theme	never-dried	1049:1059	arg1	states					1061:1066	both, dry (rewetted) and never-dried states	1024:1066	states	1061:1066	Finally, adsorbent regeneration was successfully tested for both, dry (rewetted) and never-dried states, showing improved adsorption after regeneration in the case of the never dried hydrogel structures.					
34000071	4	6	theme	adsorption	904:913	arg1	maximum					893:899	a maximum	891:899	a maximum of adsorption of 646 ± 50 mg/g	891:930	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	4	7	theme	adsorption	738:747	arg1	capacity					749:756	an adsorption capacity	735:756	an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm	735:803	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	4	8	from	successful	693:702	arg1	structure					720:728	the hydrogel structure	707:728	the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm	707:803	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	5	9	from	regeneration	1103:1114	arg1	case					1123:1126	the case	1119:1126	the case of the never dried hydrogel structures	1119:1165	Finally, adsorbent regeneration was successfully tested for both, dry (rewetted) and never-dried states, showing improved adsorption after regeneration in the case of the never dried hydrogel structures.					
34000071	4	10	from	solution	782:789	arg1	905 ± 71 mg/g					761:773	905 ± 71 mg/g	761:773	905 ± 71 mg/g from a solution with 16.6 ppm	761:803	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	4	10	from	solution	782:789	arg1	capacity					749:756	an adsorption capacity	735:756	an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm	735:803	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	3	11	theme	electrostatic	546:558	arg1	interaction					560:570	electrostatic interaction	546:570	electrostatic interaction	546:570	Herein it was noticed that the adsorption only occurred in acidic pH (5.5) where electrostatic interaction drove the adsorption, demonstrating the potential to tune the response under desired conditions only.					
34000071	0	12	theme	dependent	16:24	arg1	adsorption					26:35	Environmentally dependent adsorption	0:35	Environmentally dependent adsorption of 2,4-dichlorophenol on cellulose-chitosan	0:79	Environmentally dependent adsorption of 2,4-dichlorophenol on cellulose-chitosan self-assembled composites.					
34000071	1	13	theme	increasing	117:126	arg1	need					128:131	the increasing need	113:131	the increasing need for bio-based materials developed by environmentally friendly procedures	113:204	With the increasing need for bio-based materials developed by environmentally friendly procedures, this work shows a green method to develop shape-controlled structures from cellulose dissolving pulp coated by chitosan.					
34000071	4	14	with	structure	720:728	arg1	capacity					749:756	an adsorption capacity	735:756	an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm	735:803	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	2	15	theme	pH	438:439	arg1	conditions					441:450	different pH conditions	428:450	different pH conditions (5.5 and 9)	428:462	This material was then tested to adsorb a common and widespread pollutant, 2,4-dichlorophenol under different pH conditions (5.5 and 9).					
34000071	4	16	theme	905 ± 71 mg/g	761:773	arg1	capacity					749:756	an adsorption capacity	735:756	an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm	735:803	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	2	17	theme	different	428:436	arg1	conditions					441:450	different pH conditions	428:450	different pH conditions (5.5 and 9)	428:462	This material was then tested to adsorb a common and widespread pollutant, 2,4-dichlorophenol under different pH conditions (5.5 and 9).					
34000071	5	18	theme	adsorbent	973:981	arg1	regeneration					983:994	adsorbent regeneration	973:994	adsorbent regeneration	973:994	Finally, adsorbent regeneration was successfully tested for both, dry (rewetted) and never-dried states, showing improved adsorption after regeneration in the case of the never dried hydrogel structures.					
34000071	4	19	theme	16.6 ppm	945:952	arg1	solution					954:961	a similar 16.6 ppm solution	935:961	a similar 16.6 ppm solution	935:961	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	1	20	theme	bio-based	137:145	arg1	materials					147:155	bio-based materials	137:155	bio-based materials developed by environmentally friendly procedures	137:204	With the increasing need for bio-based materials developed by environmentally friendly procedures, this work shows a green method to develop shape-controlled structures from cellulose dissolving pulp coated by chitosan.					
34000071	4	21	theme	hydrogel	711:718	arg1	structure					720:728	the hydrogel structure	707:728	the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm	707:803	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	1	22	from	pulp	303:306	arg1	structures					266:275	shape-controlled structures	249:275	shape-controlled structures from cellulose dissolving pulp coated by chitosan	249:325	With the increasing need for bio-based materials developed by environmentally friendly procedures, this work shows a green method to develop shape-controlled structures from cellulose dissolving pulp coated by chitosan.					
34000071	1	23	theme	green	225:229	arg1	method					231:236	a green method	223:236	a green method to develop shape-controlled structures from cellulose dissolving pulp coated by chitosan	223:325	With the increasing need for bio-based materials developed by environmentally friendly procedures, this work shows a green method to develop shape-controlled structures from cellulose dissolving pulp coated by chitosan.					
34000071	0	24	theme	2,4-dichlorophenol	40:57	arg1	adsorption					26:35	Environmentally dependent adsorption	0:35	Environmentally dependent adsorption of 2,4-dichlorophenol on cellulose-chitosan	0:79	Environmentally dependent adsorption of 2,4-dichlorophenol on cellulose-chitosan self-assembled composites.					
34000071	4	25	with	solution	782:789	arg1	16.6 ppm					796:803	16.6 ppm	796:803	16.6 ppm	796:803	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	1	26	theme	shape-controlled	249:264	arg1	structures					266:275	shape-controlled structures	249:275	shape-controlled structures from cellulose dissolving pulp coated by chitosan	249:325	With the increasing need for bio-based materials developed by environmentally friendly procedures, this work shows a green method to develop shape-controlled structures from cellulose dissolving pulp coated by chitosan.					
34000071	4	27	theme	hydrogel	859:866	arg1	structures					868:877	dried hydrogel structures	853:877	dried hydrogel structures	853:877	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	2	28	theme	widespread	381:390	arg1	2,4-dichlorophenol					403:420	2,4-dichlorophenol	403:420	2,4-dichlorophenol	403:420	This material was then tested to adsorb a common and widespread pollutant, 2,4-dichlorophenol under different pH conditions (5.5 and 9).					
34000071	2	28	theme	widespread	381:390	arg1	pollutant					392:400	a common and widespread pollutant	368:400	a common and widespread pollutant	368:400	This material was then tested to adsorb a common and widespread pollutant, 2,4-dichlorophenol under different pH conditions (5.5 and 9).					
34000071	4	29	theme	646 ± 50 mg/g	918:930	arg1	adsorption					904:913	adsorption	904:913	adsorption of 646 ± 50 mg/g	904:930	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	4	30	theme	dried	853:857	arg1	structures					868:877	dried hydrogel structures	853:877	dried hydrogel structures	853:877	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	3	31	theme	acidic	524:529	arg1	pH					531:532	acidic pH (5.5)	524:538	acidic pH (5.5) where electrostatic interaction drove the adsorption	524:591	Herein it was noticed that the adsorption only occurred in acidic pH (5.5) where electrostatic interaction drove the adsorption, demonstrating the potential to tune the response under desired conditions only.					
34000071	4	32	with	possible	839:846	arg1	structures					868:877	dried hydrogel structures	853:877	dried hydrogel structures	853:877	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	4	33	theme	similar	937:943	arg1	solution					954:961	a similar 16.6 ppm solution	935:961	a similar 16.6 ppm solution	935:961	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	2	34	theme	common	370:375	arg1	2,4-dichlorophenol					403:420	2,4-dichlorophenol	403:420	2,4-dichlorophenol	403:420	This material was then tested to adsorb a common and widespread pollutant, 2,4-dichlorophenol under different pH conditions (5.5 and 9).					
34000071	2	34	theme	common	370:375	arg1	pollutant					392:400	a common and widespread pollutant	368:400	a common and widespread pollutant	368:400	This material was then tested to adsorb a common and widespread pollutant, 2,4-dichlorophenol under different pH conditions (5.5 and 9).					
34000071	4	35	from	capacity	749:756	arg1	solution					782:789	a solution	780:789	a solution with 16.6 ppm	780:803	The adsorption was successful in the hydrogel structure with an adsorption capacity of 905 ± 71 mg/g from a solution with 16.6 ppm; furthermore, adsorption was also possible with dried hydrogel structures, presenting a maximum of adsorption of 646 ± 50 mg/g in a similar 16.6 ppm solution.					
34000071	5	36	theme	improved	1077:1084	arg1	adsorption					1086:1095	improved adsorption	1077:1095	improved adsorption	1077:1095	Finally, adsorbent regeneration was successfully tested for both, dry (rewetted) and never-dried states, showing improved adsorption after regeneration in the case of the never dried hydrogel structures.					
34000071	3	37	theme	desired	649:655	arg1	conditions					657:666	desired conditions	649:666	desired conditions only	649:671	Herein it was noticed that the adsorption only occurred in acidic pH (5.5) where electrostatic interaction drove the adsorption, demonstrating the potential to tune the response under desired conditions only.					
34000071	1	38	theme	environmentally	170:184	arg1	procedures					195:204	environmentally friendly procedures	170:204	environmentally friendly procedures	170:204	With the increasing need for bio-based materials developed by environmentally friendly procedures, this work shows a green method to develop shape-controlled structures from cellulose dissolving pulp coated by chitosan.					
34000071	5	39	dep	dry	1030:1032	arg1	rewetted					1035:1042	rewetted	1035:1042	rewetted	1035:1042	Finally, adsorbent regeneration was successfully tested for both, dry (rewetted) and never-dried states, showing improved adsorption after regeneration in the case of the never dried hydrogel structures.					
34000071	1	40	theme	friendly	186:193	arg1	procedures					195:204	environmentally friendly procedures	170:204	environmentally friendly procedures	170:204	With the increasing need for bio-based materials developed by environmentally friendly procedures, this work shows a green method to develop shape-controlled structures from cellulose dissolving pulp coated by chitosan.					
34000071	1	41	theme	cellulose	282:290	arg1	pulp					303:306	cellulose dissolving pulp	282:306	cellulose dissolving pulp coated by chitosan	282:325	With the increasing need for bio-based materials developed by environmentally friendly procedures, this work shows a green method to develop shape-controlled structures from cellulose dissolving pulp coated by chitosan.					
34000071	5	42	theme	dried	1141:1145	arg1	structures					1156:1165	the never dried hydrogel structures	1131:1165	the never dried hydrogel structures	1131:1165	Finally, adsorbent regeneration was successfully tested for both, dry (rewetted) and never-dried states, showing improved adsorption after regeneration in the case of the never dried hydrogel structures.					
34000071	2	43	dep	conditions	441:450	arg1	9					461:461	9	461:461	9	461:461	This material was then tested to adsorb a common and widespread pollutant, 2,4-dichlorophenol under different pH conditions (5.5 and 9).					
34000071	2	43	dep	conditions	441:450	arg1	5.5					453:455	5.5	453:455	5.5	453:455	This material was then tested to adsorb a common and widespread pollutant, 2,4-dichlorophenol under different pH conditions (5.5 and 9).					
34000071	1	44	theme	dissolving	292:301	arg1	pulp					303:306	cellulose dissolving pulp	282:306	cellulose dissolving pulp coated by chitosan	282:325	With the increasing need for bio-based materials developed by environmentally friendly procedures, this work shows a green method to develop shape-controlled structures from cellulose dissolving pulp coated by chitosan.					
32086666	3	0	theme	chromatography-tandem	690:710	arg1	spectrometry					717:728	liquid chromatography-tandem mass spectrometry	683:728	a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method	664:746	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	0	theme	chromatography-tandem	690:710	arg1	LC-MS/MS					731:738	LC-MS/MS	731:738	LC-MS/MS	731:738	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	5	1	theme	cartilage	1488:1496	arg1	composition					1498:1508	cartilage composition	1488:1508	cartilage composition	1488:1508	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	2	2	theme	states	611:616	arg1	predictive					549:558	predictive	549:558	predictive	549:558	Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis.					
32086666	4	3	theme	chondrogenic	1063:1074	arg1	differentiation					1076:1090	The chondrogenic differentiation	1059:1090	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions	1059:1146	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	8	4	theme	chondrocytes	2002:2013	arg1	quality					1977:1983	the quality	1973:1983	the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions	1973:2072	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	6	5	theme	average	1633:1639	arg1	GAG					1641:1643	average GAG	1633:1643	average GAG	1633:1643	Additionally, hypoxic culture conditions resulted in significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones.					
32086666	1	6	theme	cartilage	213:221	arg1	ECM					245:247	ECM	245:247	ECM	245:247	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	6	theme	cartilage	213:221	arg1	matrix					237:242	cartilage extracellular matrix	213:242	cartilage extracellular matrix (ECM)	213:248	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	4	7	theme	aggregate	1169:1177	arg1	measurement					1184:1194	aggregate size measurement	1169:1194	aggregate size measurement	1169:1194	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	3	8	theme	comparative	761:771	arg1	study					773:777	a comparative study	759:777	a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures	759:1056	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	8	9	theme	oxygen	2040:2045	arg1	conditions					2063:2072	different oxygen tension culture conditions	2030:2072	different oxygen tension culture conditions	2030:2072	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	1	10	theme	matrix	237:242	arg1	Glycosaminoglycans					163:180	Glycosaminoglycans	163:180	Glycosaminoglycans (GAGs)	163:187	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	10	theme	matrix	237:242	arg1	components					199:208	major components	193:208	major components	193:208	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	2	11	theme	maturation	574:583	arg1	level					585:589	the tissue maturation level	563:589	the tissue maturation level	563:589	Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis.					
32086666	4	12	theme	different	1122:1130	arg1	tensions					1139:1146	different oxygen tensions	1122:1146	different oxygen tensions	1122:1146	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	8	13	theme	culture	2055:2061	arg1	conditions					2063:2072	different oxygen tension culture conditions	2030:2072	different oxygen tension culture conditions	2030:2072	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	1	14	theme	processes	400:408	arg1	mediators					374:382	signaling mediators	364:382	signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation	364:471	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	6	15	theme	normoxic	1705:1712	arg1	ones					1714:1717	the normoxic ones	1701:1717	the normoxic ones	1701:1717	Additionally, hypoxic culture conditions resulted in significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones.					
32086666	4	16	theme	expression	1215:1224	arg1	analysis					1226:1233	chondrogenic gene expression analysis	1197:1233	chondrogenic gene expression analysis	1197:1233	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	3	17	theme	human	875:879	arg1	marrow-					886:892	human bone marrow-	875:892	human bone marrow-	875:892	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	2	18	theme	sulfation	523:531	arg1	patterns					533:540	their disaccharide sulfation patterns	504:540	Specific GAG types as well as their disaccharide sulfation patterns	474:540	Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis.					
32086666	3	19	theme	changes	800:806	arg1	terms					782:786	terms	782:786	terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures	782:1056	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	6	20	theme	disaccharide	1652:1663	arg1	compositions					1676:1687	significantly different average GAG and CS disaccharide percentage compositions	1609:1687	significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones	1609:1717	Additionally, hypoxic culture conditions resulted in significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones.					
32086666	3	21	theme	synovial-derived	898:913	arg1	hBMSC/hSMSC					947:957	hBMSC/hSMSC	947:957	hBMSC/hSMSC	947:957	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	21	theme	synovial-derived	898:913	arg1	cells					940:944	synovial-derived mesenchymal stem/stromal cells	898:944	synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC)	898:958	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	4	22	theme	chondrogenic	1197:1208	arg1	analysis					1226:1233	chondrogenic gene expression analysis	1197:1233	chondrogenic gene expression analysis	1197:1233	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	0	23	theme	stem/stromal	116:127	arg1	cells					129:133	mesenchymal stem/stromal cells	104:133	mesenchymal stem/stromal cells	104:133	Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow-/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia.					
32086666	8	24	theme	differentiation/disease	1922:1944	arg1	states					1946:1951	cartilage tissue differentiation/disease states	1905:1951	cartilage tissue differentiation/disease states	1905:1951	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	4	25	theme	human	1297:1301	arg1	chondrocytes					1303:1314	human chondrocytes	1297:1314	human chondrocytes	1297:1314	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	3	26	theme	disaccharide	819:830	arg1	composition					832:842	disaccharide composition	819:842	disaccharide composition	819:842	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	5	27	theme	MSC	1522:1524	arg1	differentiation					1539:1553	MSC chondrogenic differentiation	1522:1553	MSC chondrogenic differentiation	1522:1553	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	1	28	theme	key	387:389	arg1	migration					428:436	migration	428:436	migration	428:436	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	28	theme	key	387:389	arg1	processes					400:408	key cellular processes	387:408	key cellular processes such as adhesion, migration, proliferation and differentiation	387:471	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	28	theme	key	387:389	arg1	adhesion					418:425	adhesion	418:425	adhesion	418:425	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	28	theme	key	387:389	arg1	differentiation					457:471	differentiation	457:471	differentiation	457:471	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	28	theme	key	387:389	arg1	proliferation					439:451	proliferation	439:451	proliferation	439:451	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	3	29	theme	stem/stromal	927:938	arg1	hBMSC/hSMSC					947:957	hBMSC/hSMSC	947:957	hBMSC/hSMSC	947:957	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	29	theme	stem/stromal	927:938	arg1	cells					940:944	synovial-derived mesenchymal stem/stromal cells	898:944	synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC)	898:958	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	2	30	theme	Specific	474:481	arg1	types					487:491	Specific GAG types	474:491	Specific GAG types as well as their disaccharide sulfation patterns	474:540	Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis.					
32086666	5	31	theme	relative	1423:1430	arg1	percentage					1432:1441	the average relative percentage	1411:1441	the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition,	1411:1509	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	0	32	theme	Glycosaminoglycan	0:16	arg1	remodeling					18:27	Glycosaminoglycan remodeling	0:27	Glycosaminoglycan remodeling during chondrogenic differentiation of human bone	0:77	Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow-/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia.					
32086666	3	33	theme	chondrogenic	966:977	arg1	differentiation					979:993	chondrogenic differentiation	966:993	chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures	966:1056	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	6	34	theme	GAG	1641:1643	arg1	compositions					1676:1687	significantly different average GAG and CS disaccharide percentage compositions	1609:1687	significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones	1609:1717	Additionally, hypoxic culture conditions resulted in significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones.					
32086666	1	35	theme	mechanical	324:333	arg1	resistance					340:349	mechanical load resistance	324:349	mechanical load resistance	324:349	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	3	36	from	changes	800:806	arg1	GAG					811:813	GAG	811:813	GAG	811:813	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	36	from	changes	800:806	arg1	composition					832:842	disaccharide composition	819:842	disaccharide composition	819:842	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	0	37	theme	human	68:72	arg1	bone					74:77	human bone	68:77	human bone	68:77	Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow-/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia.					
32086666	4	38	theme	hBMSC/hSMSC	1095:1105	arg1	differentiation					1076:1090	The chondrogenic differentiation	1059:1090	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions	1059:1146	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	3	39	from	GAG	811:813	arg1	terms					782:786	terms	782:786	terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures	782:1056	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	40	theme	liquid	683:688	arg1	spectrometry					717:728	liquid chromatography-tandem mass spectrometry	683:728	a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method	664:746	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	40	theme	liquid	683:688	arg1	LC-MS/MS					731:738	LC-MS/MS	731:738	LC-MS/MS	731:738	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	6	41	theme	hypoxic	1570:1576	arg1	conditions					1586:1595	hypoxic culture conditions	1570:1595	hypoxic culture conditions	1570:1595	Additionally, hypoxic culture conditions resulted in significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones.					
32086666	8	42	theme	GAG	1830:1832	arg1	profiles					1834:1841	the GAG profiles	1826:1841	the GAG profiles described here	1826:1856	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	8	43	theme	MSC-generated	1988:2000	arg1	chondrocytes					2002:2013	MSC-generated chondrocytes	1988:2013	MSC-generated chondrocytes obtained under different oxygen tension culture conditions	1988:2072	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	5	44	theme	sulfate	1458:1464	arg1	percentage					1432:1441	the average relative percentage	1411:1441	the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition,	1411:1509	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	3	45	theme	mass	712:715	arg1	spectrometry					717:728	liquid chromatography-tandem mass spectrometry	683:728	a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method	664:746	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	45	theme	mass	712:715	arg1	LC-MS/MS					731:738	LC-MS/MS	731:738	LC-MS/MS	731:738	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	1	46	theme	tissue	283:288	arg1	homeostasis					290:300	tissue homeostasis	283:300	tissue homeostasis	283:300	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	2	47	theme	disease	603:609	arg1	states					611:616	disease states	603:616	disease states such as osteoarthritis	603:639	Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis.					
32086666	2	47	theme	disease	603:609	arg1	osteoarthritis					626:639	osteoarthritis	626:639	osteoarthritis	626:639	Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis.					
32086666	3	48	theme	hypoxic	1023:1029	arg1	cultures					1049:1056	normoxic (21% O2) and hypoxic (5% O2) micromass cultures	1001:1056	normoxic (21% O2) and hypoxic (5% O2) micromass cultures	1001:1056	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	8	49	theme	different	2030:2038	arg1	conditions					2063:2072	different oxygen tension culture conditions	2030:2072	different oxygen tension culture conditions	2030:2072	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	3	50	dep	normoxic	1001:1008	arg1	%					1013:1013	21% O2	1011:1016	21% O2	1011:1016	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	51	theme	micromass	1039:1047	arg1	cultures					1049:1056	normoxic (21% O2) and hypoxic (5% O2) micromass cultures	1001:1056	normoxic (21% O2) and hypoxic (5% O2) micromass cultures	1001:1056	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	4	52	theme	size	1179:1182	arg1	measurement					1184:1194	aggregate size measurement	1169:1194	aggregate size measurement	1169:1194	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	8	53	theme	tension	2047:2053	arg1	conditions					2063:2072	different oxygen tension culture conditions	2030:2072	different oxygen tension culture conditions	2030:2072	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	5	54	theme	main	1476:1479	arg1	GAG					1481:1483	the main GAG	1472:1483	the main GAG in cartilage composition	1472:1508	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	5	54	theme	main	1476:1479	arg1	sulfate					1458:1464	chondroitin sulfate	1446:1464	chondroitin sulfate (CS)	1446:1469	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	6	55	theme	different	1623:1631	arg1	compositions					1676:1687	significantly different average GAG and CS disaccharide percentage compositions	1609:1687	significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones	1609:1717	Additionally, hypoxic culture conditions resulted in significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones.					
32086666	1	56	theme	extracellular	223:235	arg1	ECM					245:247	ECM	245:247	ECM	245:247	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	56	theme	extracellular	223:235	arg1	matrix					237:242	cartilage extracellular matrix	213:242	cartilage extracellular matrix (ECM)	213:248	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	4	57	theme	oxygen	1132:1137	arg1	tensions					1139:1146	different oxygen tensions	1122:1146	different oxygen tensions	1122:1146	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	7	58	theme	such	1729:1732	arg1	effect					1734:1739	such effect	1729:1739	such effect	1729:1739	However, such effect was considerably more evident for hBMSC-derived chondrogenic aggregates.					
32086666	2	59	theme	tissue	567:572	arg1	level					585:589	the tissue maturation level	563:589	the tissue maturation level	563:589	Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis.					
32086666	3	60	dep	hypoxic	1023:1029	arg1	%					1033:1033	5% O2	1032:1036	5% O2	1032:1036	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	61	link	synovial-derived	898:913	arg1	hBMSC/hSMSC					947:957	hBMSC/hSMSC	947:957	hBMSC/hSMSC	947:957	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	61	link	synovial-derived	898:913	arg1	cells					940:944	synovial-derived mesenchymal stem/stromal cells	898:944	synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC)	898:958	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	5	62	from	increase	1399:1406	arg1	percentage					1432:1441	the average relative percentage	1411:1441	the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition,	1411:1509	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	3	63	theme	temporal	791:798	arg1	changes					800:806	temporal changes	791:806	temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures	791:1056	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	7	64	link	hBMSC-derived	1775:1787	arg1	aggregates					1802:1811	hBMSC-derived chondrogenic aggregates	1775:1811	hBMSC-derived chondrogenic aggregates	1775:1811	However, such effect was considerably more evident for hBMSC-derived chondrogenic aggregates.					
32086666	4	65	theme	histological/immunofluorescence	1239:1269	arg1	staining					1271:1278	histological/immunofluorescence staining	1239:1278	histological/immunofluorescence staining	1239:1278	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	3	66	theme	bone	881:884	arg1	marrow-					886:892	human bone marrow-	875:892	human bone marrow-	875:892	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	2	67	theme	level	585:589	arg1	predictive					549:558	predictive	549:558	predictive	549:558	Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis.					
32086666	5	68	theme	compositional	1353:1365	arg1	analysis					1367:1374	the compositional analysis	1349:1374	the compositional analysis	1349:1374	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	0	69	theme	mesenchymal	104:114	arg1	cells					129:133	mesenchymal stem/stromal cells	104:133	mesenchymal stem/stromal cells	104:133	Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow-/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia.					
32086666	6	70	theme	percentage	1665:1674	arg1	compositions					1676:1687	significantly different average GAG and CS disaccharide percentage compositions	1609:1687	significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones	1609:1717	Additionally, hypoxic culture conditions resulted in significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones.					
32086666	2	71	theme	disaccharide	510:521	arg1	patterns					533:540	their disaccharide sulfation patterns	504:540	Specific GAG types as well as their disaccharide sulfation patterns	474:540	Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis.					
32086666	4	72	theme	gene	1210:1213	arg1	analysis					1226:1233	chondrogenic gene expression analysis	1197:1233	chondrogenic gene expression analysis	1197:1233	The chondrogenic differentiation of hBMSC/hSMSC cultured under different oxygen tensions was assessed through aggregate size measurement, chondrogenic gene expression analysis and histological/immunofluorescence staining in comparison to human chondrocytes.					
32086666	8	73	theme	cartilage	1905:1913	arg1	states					1946:1951	cartilage tissue differentiation/disease states	1905:1951	cartilage tissue differentiation/disease states	1905:1951	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	6	74	theme	CS	1649:1650	arg1	compositions					1676:1687	significantly different average GAG and CS disaccharide percentage compositions	1609:1687	significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones	1609:1717	Additionally, hypoxic culture conditions resulted in significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones.					
32086666	1	75	theme	major	193:197	arg1	Glycosaminoglycans					163:180	Glycosaminoglycans	163:180	Glycosaminoglycans (GAGs)	163:187	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	75	theme	major	193:197	arg1	components					199:208	major components	193:208	major components	193:208	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	3	76	theme	mesenchymal	915:925	arg1	hBMSC/hSMSC					947:957	hBMSC/hSMSC	947:957	hBMSC/hSMSC	947:957	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	76	theme	mesenchymal	915:925	arg1	cells					940:944	synovial-derived mesenchymal stem/stromal cells	898:944	synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC)	898:958	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	77	from	terms	782:786	arg1	GAG					811:813	GAG	811:813	GAG	811:813	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	77	from	terms	782:786	arg1	composition					832:842	disaccharide composition	819:842	disaccharide composition	819:842	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	5	78	theme	studied	1329:1335	arg1	conditions					1337:1346	all the studied conditions	1321:1346	all the studied conditions	1321:1346	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	1	79	theme	signaling	364:372	arg1	mediators					374:382	signaling mediators	364:382	signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation	364:471	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	8	80	theme	tissue	1915:1920	arg1	states					1946:1951	cartilage tissue differentiation/disease states	1905:1951	cartilage tissue differentiation/disease states	1905:1951	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	8	81	theme	states	1946:1951	arg1	prediction					1891:1900	the prediction	1887:1900	the prediction of cartilage tissue differentiation/disease states	1887:1951	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	5	82	theme	chondrogenic	1526:1537	arg1	differentiation					1539:1553	MSC chondrogenic differentiation	1522:1553	MSC chondrogenic differentiation	1522:1553	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	5	83	theme	average	1415:1421	arg1	percentage					1432:1441	the average relative percentage	1411:1441	the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition,	1411:1509	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	1	84	theme	cellular	391:398	arg1	migration					428:436	migration	428:436	migration	428:436	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	84	theme	cellular	391:398	arg1	processes					400:408	key cellular processes	387:408	key cellular processes such as adhesion, migration, proliferation and differentiation	387:471	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	84	theme	cellular	391:398	arg1	adhesion					418:425	adhesion	418:425	adhesion	418:425	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	84	theme	cellular	391:398	arg1	differentiation					457:471	differentiation	457:471	differentiation	457:471	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	1	84	theme	cellular	391:398	arg1	proliferation					439:451	proliferation	439:451	proliferation	439:451	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	3	85	from	study	773:777	arg1	terms					782:786	terms	782:786	terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures	782:1056	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	0	86	theme	chondrogenic	36:47	arg1	differentiation					49:63	chondrogenic differentiation	36:63	chondrogenic differentiation of human bone	36:77	Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow-/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia.					
32086666	7	87	theme	hBMSC-derived	1775:1787	arg1	aggregates					1802:1811	hBMSC-derived chondrogenic aggregates	1775:1811	hBMSC-derived chondrogenic aggregates	1775:1811	However, such effect was considerably more evident for hBMSC-derived chondrogenic aggregates.					
32086666	3	88	used	used	659:662	arg2	we					656:657	we	656:657	we	656:657	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	3	89	from	composition	832:842	arg1	terms					782:786	terms	782:786	terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures	782:1056	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	5	90	theme	notable	1391:1397	arg1	increase					1399:1406	a notable increase	1389:1406	a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition,	1389:1509	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	0	91	theme	bone	74:77	arg1	differentiation					49:63	chondrogenic differentiation	36:63	chondrogenic differentiation of human bone	36:77	Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow-/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia.					
32086666	1	92	theme	load	335:338	arg1	resistance					340:349	mechanical load resistance	324:349	mechanical load resistance	324:349	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
32086666	5	93	from	GAG	1481:1483	arg1	composition					1498:1508	cartilage composition	1488:1508	cartilage composition	1488:1508	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	2	94	theme	GAG	483:485	arg1	types					487:491	Specific GAG types	474:491	Specific GAG types as well as their disaccharide sulfation patterns	474:540	Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis.					
32086666	3	95	theme	sensitive	673:681	arg1	method					741:746	a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method	664:746	a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method	664:746	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	6	96	theme	culture	1578:1584	arg1	conditions					1586:1595	hypoxic culture conditions	1570:1595	hypoxic culture conditions	1570:1595	Additionally, hypoxic culture conditions resulted in significantly different average GAG and CS disaccharide percentage compositions compared to the normoxic ones.					
32086666	3	97	theme	normoxic	1001:1008	arg1	cultures					1049:1056	normoxic (21% O2) and hypoxic (5% O2) micromass cultures	1001:1056	normoxic (21% O2) and hypoxic (5% O2) micromass cultures	1001:1056	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	8	98	theme	new	1870:1872	arg1	insights					1874:1881	new insights	1870:1881	new insights for the prediction of cartilage tissue differentiation/disease states	1870:1951	In summary, the GAG profiles described here may provide new insights for the prediction of cartilage tissue differentiation/disease states and to characterize the quality of MSC-generated chondrocytes obtained under different oxygen tension culture conditions.					
32086666	7	99	theme	chondrogenic	1789:1800	arg1	aggregates					1802:1811	hBMSC-derived chondrogenic aggregates	1775:1811	hBMSC-derived chondrogenic aggregates	1775:1811	However, such effect was considerably more evident for hBMSC-derived chondrogenic aggregates.					
32086666	3	100	theme	spectrometry	717:728	arg1	method					741:746	a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method	664:746	a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method	664:746	In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in terms of temporal changes in GAG and disaccharide composition between tissues generated from human bone marrow- and synovial-derived mesenchymal stem/stromal cells (hBMSC/hSMSC) after chondrogenic differentiation under normoxic (21% O2) and hypoxic (5% O2) micromass cultures.					
32086666	5	101	theme	chondroitin	1446:1456	arg1	GAG					1481:1483	the main GAG	1472:1483	the main GAG in cartilage composition	1472:1508	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	5	101	theme	chondroitin	1446:1456	arg1	CS					1467:1468	CS	1467:1468	CS	1467:1468	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	5	101	theme	chondroitin	1446:1456	arg1	sulfate					1458:1464	chondroitin sulfate	1446:1464	chondroitin sulfate (CS)	1446:1469	For all the studied conditions, the compositional analysis demonstrated a notable increase in the average relative percentage of chondroitin sulfate (CS), the main GAG in cartilage composition, throughout MSC chondrogenic differentiation.					
32086666	1	102	theme	important	265:273	arg1	role					275:278	an important role	262:278	an important role	262:278	Glycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation.					
34802323	0	0	theme	tissue	104:109	arg1	regeneration					111:122	periodontal tissue regeneration	92:122	periodontal tissue regeneration	92:122	Porous membranes of quaternized chitosan composited with strontium-based nanobioceramic for periodontal tissue regeneration.					
34802323	1	1	theme	quaternary	182:191	arg1	ammonium					193:200	a degradable quaternary ammonium	169:200	a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity	169:298	This report demonstrates the development of a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity.					
34802323	0	2	theme	periodontal	92:102	arg1	regeneration					111:122	periodontal tissue regeneration	92:122	periodontal tissue regeneration	92:122	Porous membranes of quaternized chitosan composited with strontium-based nanobioceramic for periodontal tissue regeneration.					
34802323	7	3	contain	have	974:977	arg1	composite					924:932	The composite	920:932	The composite with 1.5 mg SA per 1 mL QC	920:959	The composite with 1.5 mg SA per 1 mL QC was found to have optimal qualities for GTR applications.					
34802323	7	3	contain	have	974:977	arg2	qualities					987:995	optimal qualities	979:995	optimal qualities	979:995	The composite with 1.5 mg SA per 1 mL QC was found to have optimal qualities for GTR applications.					
34802323	1	4	theme	ammonium	193:200	arg1	derivative					202:211	a degradable quaternary ammonium derivative	169:211	a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity	169:298	This report demonstrates the development of a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity.					
34802323	3	5	dep	degradation	497:507	arg1	vitro					513:517	in vitro	510:517	in vitro	510:517	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	4	6	theme	membranes	707:715	arg1	porosity					691:698	The porosity	687:698	The porosity of the membranes	687:715	The porosity of the membranes increased with increasing SA content and so did the tensile strength and the degradation.					
34802323	7	7	theme	1 mL	953:956	arg1	QC					958:959	1 mL QC	953:959	1 mL QC	953:959	The composite with 1.5 mg SA per 1 mL QC was found to have optimal qualities for GTR applications.					
34802323	3	8	theme	buffered	533:540	arg1	saline					542:547	phosphate buffered saline	523:547	phosphate buffered saline	523:547	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	1	9	theme	chitosan	216:223	arg1	derivative					202:211	a degradable quaternary ammonium derivative	169:211	a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity	169:298	This report demonstrates the development of a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity.					
34802323	1	10	theme	derivative	202:211	arg1	development					154:164	the development	150:164	the development of a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity	150:298	This report demonstrates the development of a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity.					
34802323	6	11	theme	cell	869:872	arg1	evaluation					882:891	The cell culture evaluation	865:891	The cell culture evaluation	865:891	The cell culture evaluation endorsed biocompatibility.					
34802323	7	12	theme	1.5 mg	939:944	arg1	SA					946:947	1.5 mg SA	939:947	1.5 mg SA	939:947	The composite with 1.5 mg SA per 1 mL QC was found to have optimal qualities for GTR applications.					
34802323	3	13	theme	phosphate	523:531	arg1	saline					542:547	phosphate buffered saline	523:547	phosphate buffered saline	523:547	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	4	14	theme	SA	743:744	arg1	content					746:752	SA content	743:752	SA content	743:752	The porosity of the membranes increased with increasing SA content and so did the tensile strength and the degradation.					
34802323	6	15	theme	culture	874:880	arg1	evaluation					882:891	The cell culture evaluation	865:891	The cell culture evaluation	865:891	The cell culture evaluation endorsed biocompatibility.					
34802323	2	16	theme	regeneration	399:410	arg1	applications					418:429	guided tissue regeneration (GTR) applications	385:429	guided tissue regeneration (GTR) applications	385:429	The material was made as porous membrane by solution casting and freeze drying, for guided tissue regeneration (GTR) applications.					
34802323	0	17	theme	Porous	0:5	arg1	membranes					7:15	Porous membranes	0:15	Porous membranes of quaternized chitosan	0:39	Porous membranes of quaternized chitosan composited with strontium-based nanobioceramic for periodontal tissue regeneration.					
34802323	7	18	with	composite	924:932	arg1	SA					946:947	1.5 mg SA	939:947	1.5 mg SA	939:947	The composite with 1.5 mg SA per 1 mL QC was found to have optimal qualities for GTR applications.					
34802323	2	19	theme	tissue	392:397	arg1	GTR					413:415	GTR	413:415	GTR	413:415	The material was made as porous membrane by solution casting and freeze drying, for guided tissue regeneration (GTR) applications.					
34802323	2	19	theme	tissue	392:397	arg1	regeneration					399:410	guided tissue regeneration	385:410	guided tissue regeneration (GTR) applications	385:429	The material was made as porous membrane by solution casting and freeze drying, for guided tissue regeneration (GTR) applications.					
34802323	0	20	theme	quaternized	20:30	arg1	chitosan					32:39	quaternized chitosan	20:39	quaternized chitosan	20:39	Porous membranes of quaternized chitosan composited with strontium-based nanobioceramic for periodontal tissue regeneration.					
34802323	2	21	theme	porous	326:331	arg1	membrane					333:340	porous membrane	326:340	porous membrane	326:340	The material was made as porous membrane by solution casting and freeze drying, for guided tissue regeneration (GTR) applications.					
34802323	2	21	theme	porous	326:331	arg1	material					305:312	The material	301:312	The material	301:312	The material was made as porous membrane by solution casting and freeze drying, for guided tissue regeneration (GTR) applications.					
34802323	3	22	theme	pull-out	478:485	arg1	strength					487:494	suture pull-out strength	471:494	suture pull-out strength	471:494	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	5	23	theme	pull-out	818:825	arg1	strength					827:834	The suture pull-out strength	807:834	The suture pull-out strength	807:834	The suture pull-out strength, however, showed a decrease.					
34802323	4	24	theme	tensile	769:775	arg1	strength					777:784	the tensile strength	765:784	the tensile strength	765:784	The porosity of the membranes increased with increasing SA content and so did the tensile strength and the degradation.					
34802323	3	25	theme	ligament	598:605	arg1	cells					607:611	human periodontal ligament cells	580:611	human periodontal ligament cells	580:611	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	0	26	theme	chitosan	32:39	arg1	membranes					7:15	Porous membranes	0:15	Porous membranes of quaternized chitosan	0:39	Porous membranes of quaternized chitosan composited with strontium-based nanobioceramic for periodontal tissue regeneration.					
34802323	5	27	theme	suture	811:816	arg1	strength					827:834	The suture pull-out strength	807:834	The suture pull-out strength	807:834	The suture pull-out strength, however, showed a decrease.					
34802323	7	28	theme	GTR	1001:1003	arg1	applications					1005:1016	GTR applications	1001:1016	GTR applications	1001:1016	The composite with 1.5 mg SA per 1 mL QC was found to have optimal qualities for GTR applications.					
34802323	3	29	theme	suture	471:476	arg1	strength					487:494	suture pull-out strength	471:494	suture pull-out strength	471:494	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	0	30	theme	strontium-based	57:71	arg1	nanobioceramic					73:86	strontium-based nanobioceramic	57:86	strontium-based nanobioceramic for periodontal tissue regeneration	57:122	Porous membranes of quaternized chitosan composited with strontium-based nanobioceramic for periodontal tissue regeneration.					
34802323	3	31	theme	tensile	453:459	arg1	strength					461:468	tensile strength	453:468	tensile strength	453:468	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	3	32	theme	human	580:584	arg1	cells					607:611	human periodontal ligament cells	580:611	human periodontal ligament cells	580:611	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	2	33	theme	freeze	366:371	arg1	drying					373:378	freeze drying	366:378	freeze drying	366:378	The material was made as porous membrane by solution casting and freeze drying, for guided tissue regeneration (GTR) applications.					
34802323	3	34	theme	SA	674:675	arg1	addition					677:684	SA addition	674:684	SA addition	674:684	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	3	35	theme	periodontal	586:596	arg1	cells					607:611	human periodontal ligament cells	580:611	human periodontal ligament cells	580:611	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	3	36	theme	addition	677:684	arg1	effect					645:650	the effect	641:650	the effect of derivatization and SA addition	641:684	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	1	37	theme	strontium-containing	246:265	arg1	SA					280:281	SA	280:281	SA	280:281	This report demonstrates the development of a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity.					
34802323	1	37	theme	strontium-containing	246:265	arg1	nanoapatite					267:277	strontium-containing nanoapatite	246:277	strontium-containing nanoapatite (SA) for bioactivity	246:298	This report demonstrates the development of a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity.					
34802323	3	38	theme	derivatization	655:668	arg1	effect					645:650	the effect	641:650	the effect of derivatization and SA addition	641:684	The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition.					
34802323	2	39	theme	solution	345:352	arg1	casting					354:360	solution casting	345:360	solution casting	345:360	The material was made as porous membrane by solution casting and freeze drying, for guided tissue regeneration (GTR) applications.					
34802323	7	40	theme	optimal	979:985	arg1	qualities					987:995	optimal qualities	979:995	optimal qualities	979:995	The composite with 1.5 mg SA per 1 mL QC was found to have optimal qualities for GTR applications.					
34802323	2	41	theme	guided	385:390	arg1	GTR					413:415	GTR	413:415	GTR	413:415	The material was made as porous membrane by solution casting and freeze drying, for guided tissue regeneration (GTR) applications.					
34802323	2	41	theme	guided	385:390	arg1	regeneration					399:410	guided tissue regeneration	385:410	guided tissue regeneration (GTR) applications	385:429	The material was made as porous membrane by solution casting and freeze drying, for guided tissue regeneration (GTR) applications.					
34802323	1	42	theme	degradable	171:180	arg1	ammonium					193:200	a degradable quaternary ammonium	169:200	a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity	169:298	This report demonstrates the development of a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity.					
33006399	4	0	from	changes	814:820	arg1	structure					835:843	the fiber structure	825:843	the fiber structure	825:843	Treatments promoted morphological changes in the fiber structure, resulting in materials with higher porosity, indicating partial removal of the noncellulosic fractions.					
33006399	2	1	theme	sodium	353:358	arg1	hydroxide					360:368	sodium hydroxide	353:368	sodium hydroxide	353:368	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	2	2	theme	one-step	480:487	arg1	processes					489:497	one-step processes	480:497	one-step processes for cellulose extraction from OB	480:530	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	5	3	theme	highest	1083:1089	arg1	contents					1101:1108	the highest cellulose contents	1079:1108	the highest cellulose contents	1079:1108	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	6	4	dep	%	1249:1249	arg1	hemicellulose					1251:1263	hemicellulose	1251:1263	0% hemicellulose	1248:1263	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	7	5	theme	chemical	1459:1466	arg1	reagents					1468:1475	chemical reagents	1459:1475	chemical reagents employed in the overall processes compared to multistep conventional processes	1459:1554	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	2	6	theme	present	282:288	arg1	study					290:294	The present study	278:294	The present study	278:294	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	4	7	with	materials	859:867	arg1	porosity					881:888	higher porosity	874:888	higher porosity	874:888	Treatments promoted morphological changes in the fiber structure, resulting in materials with higher porosity, indicating partial removal of the noncellulosic fractions.					
33006399	6	8	dep	%	1273:1273	arg1	lignin					1275:1280	lignin	1275:1280	19.0% lignin	1269:1280	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	4	9	theme	partial	902:908	arg1	removal					910:916	partial removal	902:916	partial removal of the noncellulosic fractions	902:947	Treatments promoted morphological changes in the fiber structure, resulting in materials with higher porosity, indicating partial removal of the noncellulosic fractions.					
33006399	6	10	theme	one-step	1198:1205	arg1	ACSH					1133:1136	ACSH	1133:1136	ACSH (processed by autoclaving with NaOH)	1133:1173	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	6	10	theme	one-step	1198:1205	arg1	treatment					1207:1215	the most effective one-step treatment	1179:1215	the most effective one-step treatment	1179:1215	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	7	11	with	materials	1362:1370	arg1	contents					1394:1401	higher cellulose contents	1377:1401	higher cellulose contents	1377:1401	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	7	11	with	materials	1362:1370	arg1	yields					1407:1412	yields	1407:1412	yields	1407:1412	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	3	12	theme	720-1000 g kg-1	737:751	arg1	recovery					725:732	a recovery	723:732	a recovery of 720-1000 g kg-1 of the original cellulose	723:777	RESULTS The processing yields ranged from 140 to 820 g kg-1 , with a recovery of 720-1000 g kg-1 of the original cellulose.					
33006399	5	13	with	use	954:956	arg1	autoclaving					1021:1031	autoclaving	1021:1031	autoclaving	1021:1031	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	4	14	theme	morphological	800:812	arg1	changes					814:820	morphological changes	800:820	morphological changes	800:820	Treatments promoted morphological changes in the fiber structure, resulting in materials with higher porosity, indicating partial removal of the noncellulosic fractions.					
33006399	7	15	theme	one-step	1322:1329	arg1	able					1347:1350	able	1347:1350	able	1347:1350	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	7	15	theme	one-step	1322:1329	arg1	treatments					1331:1340	The one-step treatments	1318:1340	The one-step treatments	1318:1340	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	2	16	theme	alkaline	390:397	arg1	peroxide					399:406	alkaline peroxide	390:406	alkaline peroxide	390:406	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	0	17	theme	cellulose-rich	101:114	arg1	material					116:123	a cellulose-rich material	99:123	a cellulose-rich material	99:123	Valorization of orange bagasse through one-step physical and chemical combined processes to obtain a cellulose-rich material.					
33006399	2	18	theme	thermal	637:643	arg1	stability					645:653	thermal stability	637:653	thermal stability	637:653	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	1	19	theme	agroindustrial	163:176	arg1	residue					178:184	an agroindustrial residue	160:184	an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose	160:275	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	1	19	theme	agroindustrial	163:176	arg1	bagasse					144:150	BACKGROUND Orange bagasse	126:150	BACKGROUND Orange bagasse (OB)	126:155	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	1	19	theme	agroindustrial	163:176	arg1	importance					204:213	great economic importance	189:213	great economic importance	189:213	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	2	20	theme	cellulose	503:511	arg1	extraction					513:522	cellulose extraction	503:522	cellulose extraction from OB	503:530	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	5	21	theme	combined	961:968	arg1	treatments					979:988	combined chemical treatments	961:988	combined chemical treatments (NaOH and peracetic acid)	961:1014	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	5	21	theme	combined	961:968	arg1	NaOH					991:994	NaOH	991:994	NaOH	991:994	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	5	21	theme	combined	961:968	arg1	acid					1010:1013	peracetic acid	1000:1013	peracetic acid	1000:1013	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	1	22	theme	cellulose	267:275	arg1	extraction					253:262	the extraction	249:262	the extraction of cellulose	249:275	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	3	23	theme	original	760:767	arg1	cellulose					769:777	the original cellulose	756:777	the original cellulose	756:777	RESULTS The processing yields ranged from 140 to 820 g kg-1 , with a recovery of 720-1000 g kg-1 of the original cellulose.					
33006399	5	24	theme	chemical	970:977	arg1	treatments					979:988	combined chemical treatments	961:988	combined chemical treatments (NaOH and peracetic acid)	961:1014	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	5	24	theme	chemical	970:977	arg1	NaOH					991:994	NaOH	991:994	NaOH	991:994	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	5	24	theme	chemical	970:977	arg1	acid					1010:1013	peracetic acid	1000:1013	peracetic acid	1000:1013	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	2	25	theme	peracetic	371:379	arg1	acid					381:384	peracetic acid	371:384	peracetic acid	371:384	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	3	26	theme	cellulose	769:777	arg1	720-1000 g kg-1					737:751	720-1000 g kg-1	737:751	720-1000 g kg-1 of the original cellulose	737:777	RESULTS The processing yields ranged from 140 to 820 g kg-1 , with a recovery of 720-1000 g kg-1 of the original cellulose.					
33006399	2	27	dep	chemical	343:350	arg1	ultrasonication					439:453	ultrasonication	439:453	ultrasonication	439:453	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	2	27	dep	chemical	343:350	arg1	hydroxide					360:368	sodium hydroxide	353:368	sodium hydroxide	353:368	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	2	27	dep	chemical	343:350	arg1	acid					381:384	peracetic acid	371:384	peracetic acid	371:384	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	2	27	dep	chemical	343:350	arg1	peroxide					399:406	alkaline peroxide	390:406	alkaline peroxide	390:406	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	2	27	dep	chemical	343:350	arg1	autoclaving					423:433	autoclaving	423:433	autoclaving	423:433	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	2	28	theme	physical	413:420	arg1	treatments					456:465	chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments	343:465	chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB	343:530	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	0	29	theme	orange	16:21	arg1	Valorization					0:11	Valorization	0:11	Valorization of orange	0:21	Valorization of orange bagasse through one-step physical and chemical combined processes to obtain a cellulose-rich material.					
33006399	6	30	with	treatment	1207:1215	arg1	index					1304:1308	a crystallinity index	1288:1308	a crystallinity index of 42%	1288:1315	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	5	31	theme	peracetic	1000:1008	arg1	treatments					979:988	combined chemical treatments	961:988	combined chemical treatments (NaOH and peracetic acid)	961:1014	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	5	31	theme	peracetic	1000:1008	arg1	acid					1010:1013	peracetic acid	1000:1013	peracetic acid	1000:1013	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	5	32	theme	cellulose	1091:1099	arg1	contents					1101:1108	the highest cellulose contents	1079:1108	the highest cellulose contents	1079:1108	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	6	33	dep	CONCLUSION	1111:1120	arg1	ACSH					1133:1136	ACSH	1133:1136	ACSH (processed by autoclaving with NaOH)	1133:1173	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	6	33	dep	CONCLUSION	1111:1120	arg1	treatment					1207:1215	the most effective one-step treatment	1179:1215	the most effective one-step treatment	1179:1215	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	1	34	theme	economic	195:202	arg1	importance					204:213	great economic importance	189:213	great economic importance	189:213	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	0	35	theme	one-step	39:46	arg1	processes					79:87	one-step physical and chemical combined processes	39:87	one-step physical and chemical combined processes	39:87	Valorization of orange bagasse through one-step physical and chemical combined processes to obtain a cellulose-rich material.					
33006399	6	36	theme	%	1315:1315	arg1	index					1304:1308	a crystallinity index	1288:1308	a crystallinity index of 42%	1288:1315	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	3	37	theme	processing	668:677	arg1	yields					679:684	The processing yields	664:684	The processing yields	664:684	RESULTS The processing yields ranged from 140 to 820 g kg-1 , with a recovery of 720-1000 g kg-1 of the original cellulose.					
33006399	7	38	theme	reaction	1424:1431	arg1	times					1433:1437	reaction times	1424:1437	reaction times	1424:1437	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	1	39	theme	importance	204:213	arg1	residue					178:184	an agroindustrial residue	160:184	an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose	160:275	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	1	39	theme	importance	204:213	arg1	bagasse					144:150	BACKGROUND Orange bagasse	126:150	BACKGROUND Orange bagasse (OB)	126:155	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	1	39	theme	importance	204:213	arg1	importance					204:213	great economic importance	189:213	great economic importance	189:213	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	7	40	theme	higher	1377:1382	arg1	contents					1394:1401	higher cellulose contents	1377:1401	higher cellulose contents	1377:1401	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	2	41	theme	treatments	456:465	arg1	combinations					327:338	different combinations	317:338	different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB	317:530	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	3	42	dep	820 g kg-1	705:714	arg1	to					702:703	to	702:703	to	702:703	RESULTS The processing yields ranged from 140 to 820 g kg-1 , with a recovery of 720-1000 g kg-1 of the original cellulose.					
33006399	4	43	theme	higher	874:879	arg1	porosity					881:888	higher porosity	874:888	higher porosity	874:888	Treatments promoted morphological changes in the fiber structure, resulting in materials with higher porosity, indicating partial removal of the noncellulosic fractions.					
33006399	2	44	theme	chemical	343:350	arg1	treatments					456:465	chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments	343:465	chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB	343:530	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	0	45	theme	physical	48:55	arg1	processes					79:87	one-step physical and chemical combined processes	39:87	one-step physical and chemical combined processes	39:87	Valorization of orange bagasse through one-step physical and chemical combined processes to obtain a cellulose-rich material.					
33006399	7	46	theme	overall	1493:1499	arg1	processes					1501:1509	the overall processes	1489:1509	the overall processes compared to multistep conventional processes	1489:1554	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	6	47	dep	ACSH	1133:1136	arg1	processed					1139:1147	processed	1139:1147	processed by autoclaving with NaOH	1139:1172	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	1	48	theme	BACKGROUND	126:135	arg1	residue					178:184	an agroindustrial residue	160:184	an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose	160:275	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	1	48	theme	BACKGROUND	126:135	arg1	OB					153:154	OB	153:154	OB	153:154	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	1	48	theme	BACKGROUND	126:135	arg1	bagasse					144:150	BACKGROUND Orange bagasse	126:150	BACKGROUND Orange bagasse (OB)	126:155	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	1	48	theme	BACKGROUND	126:135	arg1	importance					204:213	great economic importance	189:213	great economic importance	189:213	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	7	49	theme	conventional	1533:1544	arg1	processes					1546:1554	multistep conventional processes	1523:1554	multistep conventional processes	1523:1554	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	8	50	theme	Chemical	1575:1582	arg1	Industry					1584:1591	Chemical Industry	1575:1591	Chemical Industry	1575:1591	© 2020 Society of Chemical Industry.					
33006399	4	51	theme	fractions	939:947	arg1	removal					910:916	partial removal	902:916	partial removal of the noncellulosic fractions	902:947	Treatments promoted morphological changes in the fiber structure, resulting in materials with higher porosity, indicating partial removal of the noncellulosic fractions.					
33006399	1	52	theme	Orange	137:142	arg1	residue					178:184	an agroindustrial residue	160:184	an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose	160:275	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	1	52	theme	Orange	137:142	arg1	OB					153:154	OB	153:154	OB	153:154	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	1	52	theme	Orange	137:142	arg1	bagasse					144:150	BACKGROUND Orange bagasse	126:150	BACKGROUND Orange bagasse (OB)	126:155	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	1	52	theme	Orange	137:142	arg1	importance					204:213	great economic importance	189:213	great economic importance	189:213	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	5	53	theme	treatments	979:988	arg1	efficient					1042:1050	efficient	1042:1050	efficient	1042:1050	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	5	53	theme	treatments	979:988	arg1	use					954:956	The use	950:956	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving	950:1031	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	7	54	theme	cellulose	1384:1392	arg1	contents					1394:1401	higher cellulose contents	1377:1401	higher cellulose contents	1377:1401	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	4	55	theme	noncellulosic	925:937	arg1	fractions					939:947	the noncellulosic fractions	921:947	the noncellulosic fractions	921:947	Treatments promoted morphological changes in the fiber structure, resulting in materials with higher porosity, indicating partial removal of the noncellulosic fractions.					
33006399	2	56	theme	different	317:325	arg1	combinations					327:338	different combinations	317:338	different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB	317:530	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	3	57	dep	RESULTS	656:662	arg1	ranged					686:691	ranged	686:691	ranged	686:691	RESULTS The processing yields ranged from 140 to 820 g kg-1 , with a recovery of 720-1000 g kg-1 of the original cellulose.					
33006399	0	58	theme	combined	70:77	arg1	processes					79:87	one-step physical and chemical combined processes	39:87	one-step physical and chemical combined processes	39:87	Valorization of orange bagasse through one-step physical and chemical combined processes to obtain a cellulose-rich material.					
33006399	2	59	from	OB	529:530	arg1	extraction					513:522	cellulose extraction	503:522	cellulose extraction from OB	503:530	The present study aimed to investigate different combinations of chemical (sodium hydroxide, peracetic acid and alkaline peroxide) and physical (autoclaving and ultrasonication) treatments performed in one-step processes for cellulose extraction from OB and to characterize the materials obtained according to their composition, morphology, crystallinity and thermal stability.					
33006399	4	60	theme	fiber	829:833	arg1	structure					835:843	the fiber structure	825:843	the fiber structure	825:843	Treatments promoted morphological changes in the fiber structure, resulting in materials with higher porosity, indicating partial removal of the noncellulosic fractions.					
33006399	1	61	theme	great	189:193	arg1	importance					204:213	great economic importance	189:213	great economic importance	189:213	BACKGROUND Orange bagasse (OB) is an agroindustrial residue of great economic importance that has been little explored for the extraction of cellulose.					
33006399	5	62	dep	treatments	979:988	arg1	treatments					979:988	combined chemical treatments	961:988	combined chemical treatments (NaOH and peracetic acid)	961:1014	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	5	62	dep	treatments	979:988	arg1	NaOH					991:994	NaOH	991:994	NaOH	991:994	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	5	62	dep	treatments	979:988	arg1	acid					1010:1013	peracetic acid	1000:1013	peracetic acid	1000:1013	The use of combined chemical treatments (NaOH and peracetic acid) with autoclaving was more efficient for obtaining samples with the highest cellulose contents.					
33006399	6	63	theme	effective	1188:1196	arg1	ACSH					1133:1136	ACSH	1133:1136	ACSH (processed by autoclaving with NaOH)	1133:1173	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	6	63	theme	effective	1188:1196	arg1	treatment					1207:1215	the most effective one-step treatment	1179:1215	the most effective one-step treatment	1179:1215	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	6	64	theme	crystallinity	1290:1302	arg1	index					1304:1308	a crystallinity index	1288:1308	a crystallinity index of 42%	1288:1315	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	0	65	theme	chemical	61:68	arg1	processes					79:87	one-step physical and chemical combined processes	39:87	one-step physical and chemical combined processes	39:87	Valorization of orange bagasse through one-step physical and chemical combined processes to obtain a cellulose-rich material.					
33006399	7	66	theme	reagents	1468:1475	arg1	times					1433:1437	reaction times	1424:1437	reaction times	1424:1437	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	7	66	theme	reagents	1468:1475	arg1	quantity					1447:1454	the quantity	1443:1454	the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes	1443:1554	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33006399	6	67	dep	%	1235:1235	arg1	cellulose					1237:1245	cellulose	1237:1245	71.1% cellulose	1231:1245	CONCLUSION Therefore, ACSH (processed by autoclaving with NaOH) was the most effective one-step treatment, resulting in 71.1% cellulose, 0% hemicellulose and 19.0% lignin, with a crystallinity index of 42%.					
33006399	7	68	theme	multistep	1523:1531	arg1	processes					1546:1554	multistep conventional processes	1523:1554	multistep conventional processes	1523:1554	The one-step treatments were able to obtain materials with higher cellulose contents and yields, reducing reaction times and the quantity of chemical reagents employed in the overall processes compared to multistep conventional processes.					
33910714	4	0	theme	U.	439:440	arg1	sp					442:443	U. sp	439:443	U. sp	439:443	Using standardised procedures, we quantified, characterised, and compared ulvans from blade (U. australis, U. rigida, U. sp.					
33910714	4	0	theme	U.	439:440	arg1	U.					414:415	U.	414:415	U.	414:415	Using standardised procedures, we quantified, characterised, and compared ulvans from blade (U. australis, U. rigida, U. sp.					
33910714	8	1	attach	isolated	650:657	arg1	species					670:676	blade species	664:676	blade species	664:676	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	1	attach	isolated	650:657	arg2	Ulvan					644:648	Ulvan	644:648	Ulvan isolated from blade species	644:676	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	9	2	theme	properties	1003:1012	arg1	variability					968:978	the variability	964:978	the variability of the physicochemical properties of ulvan from different Ulva sources	964:1049	These results highlight the variability of the physicochemical properties of ulvan from different Ulva sources, and identifies a morphology-based division within the genus Ulva.					
33910714	8	3	theme	acid	723:726	arg1	content					728:734	iduronic acid content	714:734	iduronic acid content (IdoA = 7-18 mol%)	714:753	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	3	theme	acid	723:726	arg1	%					752:752	IdoA = 7-18 mol%	737:752	IdoA = 7-18 mol%	737:752	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	7	4	theme	ulvans	615:620	arg1	structure					602:610	structure	602:610	structure	602:610	There were distinct differences in composition and structure of ulvans between morphologies.					
33910714	7	4	theme	ulvans	615:620	arg1	composition					586:596	composition	586:596	composition	586:596	There were distinct differences in composition and structure of ulvans between morphologies.					
33910714	3	5	theme	Plenty	291:296	arg1	Bay					284:286	the Bay	280:286	the Bay of Plenty, Aotearoa New Zealand	280:318	Herein we characterise ulvan from Ulva species collected from the Bay of Plenty, Aotearoa New Zealand.					
33910714	9	6	theme	Ulva	1038:1041	arg1	sources					1043:1049	different Ulva sources	1028:1049	different Ulva sources	1028:1049	These results highlight the variability of the physicochemical properties of ulvan from different Ulva sources, and identifies a morphology-based division within the genus Ulva.					
33910714	8	7	theme	storage	806:812	arg1	moduli					814:819	storage moduli	806:819	storage moduli (G' = 0.1-6.6 Pa)	806:837	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	8	theme	IdoA	737:740	arg1	content					728:734	iduronic acid content	714:734	iduronic acid content (IdoA = 7-18 mol%)	714:753	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	8	theme	IdoA	737:740	arg1	%					752:752	IdoA = 7-18 mol%	737:752	IdoA = 7-18 mol%	737:752	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	4	9	dep	U.	414:415	arg1	australis					417:425	U. australis	414:425	U. australis	414:425	Using standardised procedures, we quantified, characterised, and compared ulvans from blade (U. australis, U. rigida, U. sp.					
33910714	2	10	from	rich	165:168	arg1	ulvan					211:215	the bioactive sulfated polysaccharide ulvan	173:215	the bioactive sulfated polysaccharide ulvan	173:215	Green seaweeds of the genus Ulva are rich in the bioactive sulfated polysaccharide ulvan.					
33910714	8	11	contain	had	678:680	arg2	%					752:752	IdoA = 7-18 mol%	737:752	IdoA = 7-18 mol%	737:752	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	11	contain	had	678:680	arg2	content					728:734	iduronic acid content	714:734	iduronic acid content (IdoA = 7-18 mol%)	714:753	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	11	contain	had	678:680	arg1	Ulvan					644:648	Ulvan	644:648	Ulvan isolated from blade species	644:676	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	11	contain	had	678:680	arg2	yields					689:694	higher yields	682:694	higher yields (14.0-19.3 %)	682:708	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	11	contain	had	678:680	arg2	%					707:707	14.0-19.3 %	697:707	14.0-19.3 %	697:707	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	12	theme	filamentous	844:854	arg1	species					856:862	filamentous species	844:862	filamentous species	844:862	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	6	13	dep	species	542:548	arg1	flexuosa					483:490	flexuosa	483:490	flexuosa	483:490	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	13	dep	species	542:548	arg1	compressa					496:504	compressa	496:504	compressa	496:504	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	13	dep	species	542:548	arg1	prolifera					510:518	prolifera	510:518	prolifera	510:518	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	13	dep	species	542:548	arg1	U.					525:526	U.	525:526	U.	525:526	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	13	dep	species	542:548	arg1	species					542:548	filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species	467:548	filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species	467:548	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	8	14	theme	7-18	744:747	arg1	content					728:734	iduronic acid content	714:734	iduronic acid content (IdoA = 7-18 mol%)	714:753	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	14	theme	7-18	744:747	arg1	%					752:752	IdoA = 7-18 mol%	737:752	IdoA = 7-18 mol%	737:752	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	3	15	from	species	257:263	arg1	ulvan					241:245	ulvan	241:245	ulvan from Ulva species collected from the Bay of Plenty, Aotearoa New Zealand	241:318	Herein we characterise ulvan from Ulva species collected from the Bay of Plenty, Aotearoa New Zealand.					
33910714	3	16	theme	New	308:310	arg1	Zealand					312:318	Aotearoa New Zealand	299:318	Aotearoa New Zealand	299:318	Herein we characterise ulvan from Ulva species collected from the Bay of Plenty, Aotearoa New Zealand.					
33910714	3	16	theme	New	308:310	arg1	Plenty					291:296	Plenty	291:296	Plenty	291:296	Herein we characterise ulvan from Ulva species collected from the Bay of Plenty, Aotearoa New Zealand.					
33910714	2	17	theme	Green	128:132	arg1	seaweeds					134:141	Green seaweeds	128:141	Green seaweeds of the genus Ulva	128:159	Green seaweeds of the genus Ulva are rich in the bioactive sulfated polysaccharide ulvan.					
33910714	1	18	theme	chemical	54:61	arg1	properties					75:84	the chemical and gelling properties	50:84	the chemical and gelling properties of	50:87	A comparative assessment of the chemical and gelling properties of ulvan from blade and filamentous Ulva.					
33910714	8	19	theme	=	742:742	arg1	content					728:734	iduronic acid content	714:734	iduronic acid content (IdoA = 7-18 mol%)	714:753	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	19	theme	=	742:742	arg1	%					752:752	IdoA = 7-18 mol%	737:752	IdoA = 7-18 mol%	737:752	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	9	20	dep	genus	1106:1110	arg1	Ulva					1112:1115	the genus Ulva	1102:1115	the genus Ulva	1102:1115	These results highlight the variability of the physicochemical properties of ulvan from different Ulva sources, and identifies a morphology-based division within the genus Ulva.					
33910714	2	21	from	ulvan	211:215	arg1	rich					165:168	rich	165:168	rich	165:168	Green seaweeds of the genus Ulva are rich in the bioactive sulfated polysaccharide ulvan.					
33910714	9	22	from	properties	1003:1012	arg1	sources					1043:1049	different Ulva sources	1028:1049	different Ulva sources	1028:1049	These results highlight the variability of the physicochemical properties of ulvan from different Ulva sources, and identifies a morphology-based division within the genus Ulva.					
33910714	4	23	theme	standardised	327:338	arg1	procedures					340:349	standardised procedures	327:349	standardised procedures	327:349	Using standardised procedures, we quantified, characterised, and compared ulvans from blade (U. australis, U. rigida, U. sp.					
33910714	1	24	theme	gelling	67:73	arg1	properties					75:84	the chemical and gelling properties	50:84	the chemical and gelling properties of	50:87	A comparative assessment of the chemical and gelling properties of ulvan from blade and filamentous Ulva.					
33910714	8	25	theme	mol	749:751	arg1	content					728:734	iduronic acid content	714:734	iduronic acid content (IdoA = 7-18 mol%)	714:753	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	25	theme	mol	749:751	arg1	%					752:752	IdoA = 7-18 mol%	737:752	IdoA = 7-18 mol%	737:752	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	9	26	theme	morphology-based	1069:1084	arg1	division					1086:1093	a morphology-based division	1067:1093	a morphology-based division	1067:1093	These results highlight the variability of the physicochemical properties of ulvan from different Ulva sources, and identifies a morphology-based division within the genus Ulva.					
33910714	1	27	theme	properties	75:84	arg1	assessment					36:45	A comparative assessment	22:45	A comparative assessment of the chemical and gelling properties of	22:87	A comparative assessment of the chemical and gelling properties of ulvan from blade and filamentous Ulva.					
33910714	3	28	theme	Ulva	252:255	arg1	species					257:263	Ulva species	252:263	Ulva species collected from the Bay of Plenty, Aotearoa New Zealand	252:318	Herein we characterise ulvan from Ulva species collected from the Bay of Plenty, Aotearoa New Zealand.					
33910714	8	29	theme	=	825:825	arg1	G					822:822	G'	822:823	G' = 0.1-6.6 Pa	822:836	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	30	dep	moduli	814:819	arg1	G					822:822	G'	822:823	G' = 0.1-6.6 Pa	822:836	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	2	31	theme	Ulva	156:159	arg1	seaweeds					134:141	Green seaweeds	128:141	Green seaweeds of the genus Ulva	128:159	Green seaweeds of the genus Ulva are rich in the bioactive sulfated polysaccharide ulvan.					
33910714	8	32	theme	lower	760:764	arg1	weight					776:781	lower molecular weight	760:781	lower molecular weight (Mw = 190-254 kDa)	760:800	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	33	dep	=	825:825	arg1	Pa					835:836	0.1-6.6 Pa	827:836	0.1-6.6 Pa	827:836	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	34	theme	higher	682:687	arg1	yields					689:694	higher yields	682:694	higher yields (14.0-19.3 %)	682:708	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	34	theme	higher	682:687	arg1	%					707:707	14.0-19.3 %	697:707	14.0-19.3 %	697:707	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	35	dep	weight	776:781	arg1	Mw					784:785	Mw	784:785	Mw = 190-254 kDa	784:799	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	9	36	from	sources	1043:1049	arg1	properties					1003:1012	the physicochemical properties	983:1012	the physicochemical properties of ulvan from different Ulva sources	983:1049	These results highlight the variability of the physicochemical properties of ulvan from different Ulva sources, and identifies a morphology-based division within the genus Ulva.					
33910714	9	36	from	sources	1043:1049	arg1	ulvan					1017:1021	ulvan	1017:1021	ulvan from different Ulva sources	1017:1049	These results highlight the variability of the physicochemical properties of ulvan from different Ulva sources, and identifies a morphology-based division within the genus Ulva.					
33910714	4	37	from	blade	407:411	arg1	ulvans					395:400	ulvans	395:400	ulvans from blade	395:411	Using standardised procedures, we quantified, characterised, and compared ulvans from blade (U. australis, U. rigida, U. sp.					
33910714	2	38	theme	polysaccharide	196:209	arg1	ulvan					211:215	the bioactive sulfated polysaccharide ulvan	173:215	the bioactive sulfated polysaccharide ulvan	173:215	Green seaweeds of the genus Ulva are rich in the bioactive sulfated polysaccharide ulvan.					
33910714	7	39	from	differences	571:581	arg1	structure					602:610	structure	602:610	structure	602:610	There were distinct differences in composition and structure of ulvans between morphologies.					
33910714	7	39	from	differences	571:581	arg1	composition					586:596	composition	586:596	composition	586:596	There were distinct differences in composition and structure of ulvans between morphologies.					
33910714	8	40	theme	4-7	892:894	arg1	mol					896:898	mol	896:898	mol	896:898	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	2	41	theme	sulfated	187:194	arg1	ulvan					211:215	the bioactive sulfated polysaccharide ulvan	173:215	the bioactive sulfated polysaccharide ulvan	173:215	Green seaweeds of the genus Ulva are rich in the bioactive sulfated polysaccharide ulvan.					
33910714	8	42	theme	molecular	766:774	arg1	weight					776:781	lower molecular weight	760:781	lower molecular weight (Mw = 190-254 kDa)	760:800	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	9	43	theme	physicochemical	987:1001	arg1	properties					1003:1012	the physicochemical properties	983:1012	the physicochemical properties of ulvan from different Ulva sources	983:1049	These results highlight the variability of the physicochemical properties of ulvan from different Ulva sources, and identifies a morphology-based division within the genus Ulva.					
33910714	2	44	theme	bioactive	177:185	arg1	ulvan					211:215	the bioactive sulfated polysaccharide ulvan	173:215	the bioactive sulfated polysaccharide ulvan	173:215	Green seaweeds of the genus Ulva are rich in the bioactive sulfated polysaccharide ulvan.					
33910714	9	45	theme	different	1028:1036	arg1	sources					1043:1049	different Ulva sources	1028:1049	different Ulva sources	1028:1049	These results highlight the variability of the physicochemical properties of ulvan from different Ulva sources, and identifies a morphology-based division within the genus Ulva.					
33910714	7	46	theme	distinct	562:569	arg1	differences					571:581	distinct differences	562:581	distinct differences in composition and structure of ulvans between morphologies	562:641	There were distinct differences in composition and structure of ulvans between morphologies.					
33910714	6	47	theme	Ulva	537:540	arg1	flexuosa					483:490	flexuosa	483:490	flexuosa	483:490	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	47	theme	Ulva	537:540	arg1	compressa					496:504	compressa	496:504	compressa	496:504	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	47	theme	Ulva	537:540	arg1	prolifera					510:518	prolifera	510:518	prolifera	510:518	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	47	theme	Ulva	537:540	arg1	U.					525:526	U.	525:526	U.	525:526	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	47	theme	Ulva	537:540	arg1	species					542:548	filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species	467:548	filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species	467:548	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	8	48	theme	blade	664:668	arg1	species					670:676	blade species	664:676	blade species	664:676	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	49	theme	iduronic	714:721	arg1	content					728:734	iduronic acid content	714:734	iduronic acid content (IdoA = 7-18 mol%)	714:753	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	49	theme	iduronic	714:721	arg1	%					752:752	IdoA = 7-18 mol%	737:752	IdoA = 7-18 mol%	737:752	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	6	50	theme	filamentous	467:477	arg1	flexuosa					483:490	flexuosa	483:490	flexuosa	483:490	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	50	theme	filamentous	467:477	arg1	compressa					496:504	compressa	496:504	compressa	496:504	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	50	theme	filamentous	467:477	arg1	prolifera					510:518	prolifera	510:518	prolifera	510:518	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	50	theme	filamentous	467:477	arg1	U.					525:526	U.	525:526	U.	525:526	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	6	50	theme	filamentous	467:477	arg1	species					542:548	filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species	467:548	filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species	467:548	and filamentous (U. flexuosa, U. compressa, U. prolifera, and U. ralfsii) Ulva species.					
33910714	9	51	theme	ulvan	1017:1021	arg1	properties					1003:1012	the physicochemical properties	983:1012	the physicochemical properties of ulvan from different Ulva sources	983:1049	These results highlight the variability of the physicochemical properties of ulvan from different Ulva sources, and identifies a morphology-based division within the genus Ulva.					
33910714	1	52	theme	comparative	24:34	arg1	assessment					36:45	A comparative assessment	22:45	A comparative assessment of the chemical and gelling properties of	22:87	A comparative assessment of the chemical and gelling properties of ulvan from blade and filamentous Ulva.					
33910714	8	53	theme	=	787:787	arg1	Mw					784:785	Mw	784:785	Mw = 190-254 kDa	784:799	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	8	54	dep	=	787:787	arg1	kDa					797:799	190-254 kDa	789:799	190-254 kDa	789:799	Ulvan isolated from blade species had higher yields (14.0-19.3 %) and iduronic acid content (IdoA = 7-18 mol%), and lower molecular weight (Mw = 190-254 kDa) and storage moduli (G' = 0.1-6.6 Pa) than filamentous species (yield = 7.2-14.6 %; IdoA = 4-7 mol%; Mw = 260-406 kDa; G' = 22.7-74.2 Pa).					
33910714	4	55	dep	compared	386:393	arg1	rigida					431:436	rigida	431:436	rigida	431:436	Using standardised procedures, we quantified, characterised, and compared ulvans from blade (U. australis, U. rigida, U. sp.					
33910714	4	55	dep	compared	386:393	arg1	sp					442:443	U. sp	439:443	U. sp	439:443	Using standardised procedures, we quantified, characterised, and compared ulvans from blade (U. australis, U. rigida, U. sp.					
33910714	4	55	dep	compared	386:393	arg1	U.					414:415	U.	414:415	U.	414:415	Using standardised procedures, we quantified, characterised, and compared ulvans from blade (U. australis, U. rigida, U. sp.					
33910714	3	56	theme	Aotearoa	299:306	arg1	Zealand					312:318	Aotearoa New Zealand	299:318	Aotearoa New Zealand	299:318	Herein we characterise ulvan from Ulva species collected from the Bay of Plenty, Aotearoa New Zealand.					
33910714	3	56	theme	Aotearoa	299:306	arg1	Plenty					291:296	Plenty	291:296	Plenty	291:296	Herein we characterise ulvan from Ulva species collected from the Bay of Plenty, Aotearoa New Zealand.					
33910714	5	57	theme	Ulva	453:456	arg1	sp					458:459	Ulva sp	453:459	Ulva sp	453:459	B, and Ulva sp.)					
32178281	8	0	theme	molecular	1380:1388	arg1	studies					1390:1396	future molecular studies	1373:1396	future molecular studies on this ascomycete	1373:1415	Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in D. teres, thus serving as a basis for future molecular studies on this ascomycete.					
32178281	2	1	theme	fungi	311:315	arg1	development					296:306	development	296:306	development	296:306	The cell wall is a crucial structure for the growth and development of fungi.					
32178281	2	1	theme	fungi	311:315	arg1	growth					285:290	growth	285:290	growth	285:290	The cell wall is a crucial structure for the growth and development of fungi.					
32178281	1	2	theme	important	213:221	arg1	impact					232:237	an important economic impact	210:237	an important economic impact	210:237	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	6	3	from	expression	848:857	arg1	spores					886:891	D. teres spores	877:891	D. teres spores	877:891	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	3	from	expression	848:857	arg1	media					944:948	different media	934:948	different media	934:948	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	3	from	expression	848:857	arg1	mycelium					904:911	the mycelium	900:911	the mycelium after cultivation on different media	900:948	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	3	from	expression	848:857	arg1	presence					969:976	the presence	965:976	the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB)	965:1064	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	0	4	from	Analysis	11:18	arg1	Fungus					71:76	the Plant Pathogenic Fungus	50:76	the Plant Pathogenic Fungus	50:76	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus Drechslera teres.					
32178281	6	5	theme	phytofirmans	998:1009	arg1	PsJN					1018:1021	Paraburkholderia phytofirmans strain PsJN	981:1021	Paraburkholderia phytofirmans strain PsJN	981:1021	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	5	theme	phytofirmans	998:1009	arg1	bacterium					1049:1057	a plant growth-promoting bacterium	1024:1057	a plant growth-promoting bacterium (PGPB)	1024:1064	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	1	6	theme	economic	223:230	arg1	impact					232:237	an important economic impact	210:237	an important economic impact	210:237	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	7	7	theme	higher	1111:1116	arg1	expression					1123:1132	higher gene expression	1111:1132	higher gene expression in the spores and in the mycelium	1111:1166	The targeted gene expression analysis shows higher gene expression in the spores and in the mycelium with the application of PGPB.					
32178281	5	8	theme	key	596:598	arg1	genes					600:604	key genes	596:604	key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase	596:806	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	3	9	theme	wall	343:346	arg1	structure					348:356	cell wall structure	338:356	cell wall structure	338:356	Thus, understanding cell wall structure, composition and biosynthesis can help in designing new strategies for pest management.					
32178281	7	10	theme	gene	1118:1121	arg1	expression					1123:1132	higher gene expression	1111:1132	higher gene expression in the spores and in the mycelium	1111:1166	The targeted gene expression analysis shows higher gene expression in the spores and in the mycelium with the application of PGPB.					
32178281	6	11	from	media	944:948	arg1	expression					848:857	the differential expression	831:857	the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB)	831:1064	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	12	theme	genes	868:872	arg1	expression					848:857	the differential expression	831:857	the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB)	831:1064	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	13	theme	strain	1011:1016	arg1	PsJN					1018:1021	Paraburkholderia phytofirmans strain PsJN	981:1021	Paraburkholderia phytofirmans strain PsJN	981:1021	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	13	theme	strain	1011:1016	arg1	bacterium					1049:1057	a plant growth-promoting bacterium	1024:1057	a plant growth-promoting bacterium (PGPB)	1024:1064	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	5	14	theme	mitogen-activated	775:791	arg1	kinase					801:806	a mitogen-activated protein kinase	773:806	a mitogen-activated protein kinase	773:806	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	6	15	from	mycelium	904:911	arg1	media					944:948	different media	934:948	different media	934:948	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	5	16	theme	endo/exoglucanases	750:767	arg1	synthesis/remodeling					622:641	the synthesis/remodeling	618:641	the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases	618:767	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	16	theme	endo/exoglucanases	750:767	arg1	kinase					801:806	a mitogen-activated protein kinase	773:806	a mitogen-activated protein kinase	773:806	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	2	17	theme	cell	244:247	arg1	wall					249:252	The cell wall	240:252	The cell wall	240:252	The cell wall is a crucial structure for the growth and development of fungi.					
32178281	2	17	theme	cell	244:247	arg1	structure					267:275	a crucial structure	257:275	a crucial structure for the growth and development of fungi	257:315	The cell wall is a crucial structure for the growth and development of fungi.					
32178281	8	18	theme	reference	1290:1298	arg1	genes					1300:1304	the most suitable reference genes	1272:1304	the most suitable reference genes	1272:1304	Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in D. teres, thus serving as a basis for future molecular studies on this ascomycete.					
32178281	1	19	theme	responsible	142:152	arg1	ascomycete					130:139	an ascomycete	127:139	an ascomycete	127:139	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	1	19	theme	responsible	142:152	arg1	teres					107:111	Drechslera teres	96:111	Drechslera teres (D. teres)	96:122	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	5	20	theme	glucan	721:726	arg1	synthases					728:736	mixed-linkage glucan synthases	707:736	mixed-linkage glucan synthases	707:736	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	20	theme	glucan	721:726	arg1	polysaccharides					656:670	cell wall polysaccharides	646:670	cell wall polysaccharides	646:670	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	6	21	theme	plant	1026:1030	arg1	PsJN					1018:1021	Paraburkholderia phytofirmans strain PsJN	981:1021	Paraburkholderia phytofirmans strain PsJN	981:1021	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	21	theme	plant	1026:1030	arg1	PGPB					1060:1063	PGPB	1060:1063	PGPB	1060:1063	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	21	theme	plant	1026:1030	arg1	bacterium					1049:1057	a plant growth-promoting bacterium	1024:1057	a plant growth-promoting bacterium (PGPB)	1024:1064	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	0	22	dep	teres	89:93	arg1	Analysis					11:18	Expression Analysis	0:18	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus	0:76	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus Drechslera teres.					
32178281	4	23	dep	severity	458:465	arg1	the					454:456	the	454:456	the	454:456	Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in D. teres.					
32178281	3	24	theme	cell	338:341	arg1	structure					348:356	cell wall structure	338:356	cell wall structure	338:356	Thus, understanding cell wall structure, composition and biosynthesis can help in designing new strategies for pest management.					
32178281	5	25	dep	-glucan	695:701	arg1	1,3					691:693	1,3	691:693	1,3	691:693	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	26	theme	β-	688:689	arg1	-glucan					695:701	β-(1,3)-glucan	688:701	β-(1,3)-glucan	688:701	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	26	theme	β-	688:689	arg1	polysaccharides					656:670	cell wall polysaccharides	646:670	cell wall polysaccharides	646:670	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	7	27	theme	gene	1080:1083	arg1	analysis					1096:1103	The targeted gene expression analysis	1067:1103	The targeted gene expression analysis	1067:1103	The targeted gene expression analysis shows higher gene expression in the spores and in the mycelium with the application of PGPB.					
32178281	1	28	theme	net	158:160	arg1	blotch					162:167	net blotch	158:167	net blotch	158:167	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	1	28	theme	net	158:160	arg1	disease					194:200	the most serious barley disease	170:200	the most serious barley disease causing an important economic impact	170:237	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	0	29	theme	Expression	0:9	arg1	Analysis					11:18	Expression Analysis	0:18	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus	0:76	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus Drechslera teres.					
32178281	7	30	from	expression	1123:1132	arg1	mycelium					1159:1166	the mycelium	1155:1166	the mycelium	1155:1166	The targeted gene expression analysis shows higher gene expression in the spores and in the mycelium with the application of PGPB.					
32178281	7	30	from	expression	1123:1132	arg1	spores					1141:1146	the spores	1137:1146	the spores	1137:1146	The targeted gene expression analysis shows higher gene expression in the spores and in the mycelium with the application of PGPB.					
32178281	0	31	theme	Cell	23:26	arg1	Genes					41:45	Cell Wall-Related Genes	23:45	Cell Wall-Related Genes	23:45	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus Drechslera teres.					
32178281	2	32	theme	crucial	259:265	arg1	wall					249:252	The cell wall	240:252	The cell wall	240:252	The cell wall is a crucial structure for the growth and development of fungi.					
32178281	2	32	theme	crucial	259:265	arg1	structure					267:275	a crucial structure	257:275	a crucial structure for the growth and development of fungi	257:315	The cell wall is a crucial structure for the growth and development of fungi.					
32178281	7	33	theme	targeted	1071:1078	arg1	analysis					1096:1103	The targeted gene expression analysis	1067:1103	The targeted gene expression analysis	1067:1103	The targeted gene expression analysis shows higher gene expression in the spores and in the mycelium with the application of PGPB.					
32178281	5	34	theme	cell	646:649	arg1	chitin					680:685	chitin	680:685	chitin	680:685	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	34	theme	cell	646:649	arg1	-glucan					695:701	β-(1,3)-glucan	688:701	β-(1,3)-glucan	688:701	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	34	theme	cell	646:649	arg1	synthases					728:736	mixed-linkage glucan synthases	707:736	mixed-linkage glucan synthases	707:736	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	34	theme	cell	646:649	arg1	polysaccharides					656:670	cell wall polysaccharides	646:670	cell wall polysaccharides	646:670	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	8	35	theme	suitable	1281:1288	arg1	genes					1300:1304	the most suitable reference genes	1272:1304	the most suitable reference genes	1272:1304	Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in D. teres, thus serving as a basis for future molecular studies on this ascomycete.					
32178281	4	36	theme	D.	567:568	arg1	teres					570:574	D. teres	567:574	D. teres	567:574	Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in D. teres.					
32178281	0	37	theme	Genes	41:45	arg1	Analysis					11:18	Expression Analysis	0:18	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus	0:76	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus Drechslera teres.					
32178281	8	38	dep	D.	1335:1336	arg1	teres					1338:1342	D. teres	1335:1342	D. teres	1335:1342	Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in D. teres, thus serving as a basis for future molecular studies on this ascomycete.					
32178281	5	39	theme	wall	651:654	arg1	chitin					680:685	chitin	680:685	chitin	680:685	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	39	theme	wall	651:654	arg1	-glucan					695:701	β-(1,3)-glucan	688:701	β-(1,3)-glucan	688:701	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	39	theme	wall	651:654	arg1	synthases					728:736	mixed-linkage glucan synthases	707:736	mixed-linkage glucan synthases	707:736	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	39	theme	wall	651:654	arg1	polysaccharides					656:670	cell wall polysaccharides	646:670	cell wall polysaccharides	646:670	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	7	40	theme	expression	1085:1094	arg1	analysis					1096:1103	The targeted gene expression analysis	1067:1103	The targeted gene expression analysis	1067:1103	The targeted gene expression analysis shows higher gene expression in the spores and in the mycelium with the application of PGPB.					
32178281	8	41	from	results	1324:1330	arg1	D.					1335:1336	D.	1335:1336	D.	1335:1336	Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in D. teres, thus serving as a basis for future molecular studies on this ascomycete.					
32178281	7	42	theme	PGPB	1192:1195	arg1	application					1177:1187	the application	1173:1187	the application of PGPB	1173:1195	The targeted gene expression analysis shows higher gene expression in the spores and in the mycelium with the application of PGPB.					
32178281	0	43	theme	Wall-Related	28:39	arg1	Genes					41:45	Cell Wall-Related Genes	23:45	Cell Wall-Related Genes	23:45	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus Drechslera teres.					
32178281	6	44	theme	D.	877:878	arg1	spores					886:891	D. teres spores	877:891	D. teres spores	877:891	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	4	45	theme	first	514:518	arg1	this					502:505	this	502:505	this	502:505	Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in D. teres.					
32178281	4	45	theme	first	514:518	arg1	study					520:524	the first study	510:524	the first study analyzing the cell wall-related genes in D. teres	510:574	Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in D. teres.					
32178281	6	46	dep	D.	877:878	arg1	teres					880:884	D. teres	877:884	D. teres spores	877:891	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	47	theme	Paraburkholderia	981:996	arg1	PsJN					1018:1021	Paraburkholderia phytofirmans strain PsJN	981:1021	Paraburkholderia phytofirmans strain PsJN	981:1021	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	47	theme	Paraburkholderia	981:996	arg1	bacterium					1049:1057	a plant growth-promoting bacterium	1024:1057	a plant growth-promoting bacterium (PGPB)	1024:1064	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	4	48	theme	economic	471:478	arg1	impact					480:485	economic impact	471:485	economic impact	471:485	Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in D. teres.					
32178281	3	49	theme	new	410:412	arg1	strategies					414:423	new strategies	410:423	new strategies for pest management	410:443	Thus, understanding cell wall structure, composition and biosynthesis can help in designing new strategies for pest management.					
32178281	5	50	theme	mixed-linkage	707:719	arg1	synthases					728:736	mixed-linkage glucan synthases	707:736	mixed-linkage glucan synthases	707:736	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	50	theme	mixed-linkage	707:719	arg1	polysaccharides					656:670	cell wall polysaccharides	646:670	cell wall polysaccharides	646:670	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	8	51	theme	qPCR	1319:1322	arg1	results					1324:1330	qPCR results	1319:1330	qPCR results in D. teres	1319:1342	Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in D. teres, thus serving as a basis for future molecular studies on this ascomycete.					
32178281	8	52	theme	key	1216:1218	arg1	genes					1238:1242	key cell-wall-related genes	1216:1242	key cell-wall-related genes	1216:1242	Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in D. teres, thus serving as a basis for future molecular studies on this ascomycete.					
32178281	5	53	theme	polysaccharides	656:670	arg1	synthesis/remodeling					622:641	the synthesis/remodeling	618:641	the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases	618:767	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	5	53	theme	polysaccharides	656:670	arg1	kinase					801:806	a mitogen-activated protein kinase	773:806	a mitogen-activated protein kinase	773:806	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	4	54	theme	blotch	494:499	arg1	severity					458:465	severity	458:465	severity	458:465	Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in D. teres.					
32178281	4	54	theme	blotch	494:499	arg1	impact					480:485	economic impact	471:485	economic impact	471:485	Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in D. teres.					
32178281	6	55	theme	differential	835:846	arg1	expression					848:857	the differential expression	831:857	the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB)	831:1064	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	56	theme	growth-promoting	1032:1047	arg1	PsJN					1018:1021	Paraburkholderia phytofirmans strain PsJN	981:1021	Paraburkholderia phytofirmans strain PsJN	981:1021	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	56	theme	growth-promoting	1032:1047	arg1	PGPB					1060:1063	PGPB	1060:1063	PGPB	1060:1063	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	6	56	theme	growth-promoting	1032:1047	arg1	bacterium					1049:1057	a plant growth-promoting bacterium	1024:1057	a plant growth-promoting bacterium (PGPB)	1024:1064	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	4	57	theme	net	490:492	arg1	blotch					494:499	net blotch	490:499	net blotch	490:499	Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in D. teres.					
32178281	3	58	theme	pest	429:432	arg1	management					434:443	pest management	429:443	pest management	429:443	Thus, understanding cell wall structure, composition and biosynthesis can help in designing new strategies for pest management.					
32178281	1	59	theme	Drechslera	96:105	arg1	teres					117:121	teres	117:121	teres	117:121	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	1	59	theme	Drechslera	96:105	arg1	ascomycete					130:139	an ascomycete	127:139	an ascomycete	127:139	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	1	59	theme	Drechslera	96:105	arg1	teres					107:111	Drechslera teres	96:111	Drechslera teres (D. teres)	96:122	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	1	60	theme	barley	187:192	arg1	blotch					162:167	net blotch	158:167	net blotch	158:167	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	1	60	theme	barley	187:192	arg1	disease					194:200	the most serious barley disease	170:200	the most serious barley disease causing an important economic impact	170:237	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	8	61	theme	future	1373:1378	arg1	studies					1390:1396	future molecular studies	1373:1396	future molecular studies on this ascomycete	1373:1415	Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in D. teres, thus serving as a basis for future molecular studies on this ascomycete.					
32178281	8	62	from	studies	1390:1396	arg1	ascomycete					1406:1415	this ascomycete	1401:1415	this ascomycete	1401:1415	Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in D. teres, thus serving as a basis for future molecular studies on this ascomycete.					
32178281	6	63	theme	PsJN	1018:1021	arg1	presence					969:976	the presence	965:976	the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB)	965:1064	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	4	64	theme	cell	540:543	arg1	genes					558:562	the cell wall-related genes	536:562	the cell wall-related genes	536:562	Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in D. teres.					
32178281	0	65	theme	Pathogenic	60:69	arg1	Fungus					71:76	the Plant Pathogenic Fungus	50:76	the Plant Pathogenic Fungus	50:76	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus Drechslera teres.					
32178281	1	66	theme	serious	179:185	arg1	blotch					162:167	net blotch	158:167	net blotch	158:167	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	1	66	theme	serious	179:185	arg1	disease					194:200	the most serious barley disease	170:200	the most serious barley disease causing an important economic impact	170:237	Drechslera teres (D. teres) is an ascomycete, responsible for net blotch, the most serious barley disease causing an important economic impact.					
32178281	8	67	theme	cell-wall-related	1220:1236	arg1	genes					1238:1242	key cell-wall-related genes	1216:1242	key cell-wall-related genes	1216:1242	Besides analyzing key cell-wall-related genes, this study also identifies the most suitable reference genes to normalize qPCR results in D. teres, thus serving as a basis for future molecular studies on this ascomycete.					
32178281	5	68	theme	protein	793:799	arg1	kinase					801:806	a mitogen-activated protein kinase	773:806	a mitogen-activated protein kinase	773:806	We have identified key genes involved in the synthesis/remodeling of cell wall polysaccharides, namely chitin, β-(1,3)-glucan and mixed-linkage glucan synthases, as well as endo/exoglucanases and a mitogen-activated protein kinase.					
32178281	6	69	theme	different	934:942	arg1	media					944:948	different media	934:948	different media	934:948	We have also analyzed the differential expression of these genes in D. teres spores and in the mycelium after cultivation on different media, as well as in the presence of Paraburkholderia phytofirmans strain PsJN, a plant growth-promoting bacterium (PGPB).					
32178281	0	70	theme	Plant	54:58	arg1	Fungus					71:76	the Plant Pathogenic Fungus	50:76	the Plant Pathogenic Fungus	50:76	Expression Analysis of Cell Wall-Related Genes in the Plant Pathogenic Fungus Drechslera teres.					
32178281	4	71	theme	wall-related	545:556	arg1	genes					558:562	the cell wall-related genes	536:562	the cell wall-related genes	536:562	Despite the severity and economic impact of net blotch, this is the first study analyzing the cell wall-related genes in D. teres.					
33091476	4	0	from	rate	674:677	arg1	clay					703:706	kaolin clay	696:706	kaolin clay	696:706	The flocculation rate of the U9-EPS on kaolin clay reached 87.9%, which was significantly higher than that (74.7%) of the V19-EPS while the decolorization rate of Congo Red (CR) by the U9-EPS reached 94.3%, which was significantly higher than that of CR by the V19-EPS (86.23%).					
33091476	1	1	theme	flocculating	237:248	arg1	activity					250:257	its flocculating activity	233:257	its flocculating activity	233:257	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	4	2	theme	decolorization	797:810	arg1	rate					812:815	the decolorization rate	793:815	the decolorization rate of Congo Red (CR) by the U9-EPS	793:847	The flocculation rate of the U9-EPS on kaolin clay reached 87.9%, which was significantly higher than that (74.7%) of the V19-EPS while the decolorization rate of Congo Red (CR) by the U9-EPS reached 94.3%, which was significantly higher than that of CR by the V19-EPS (86.23%).					
33091476	2	3	theme	U9	352:353	arg1	strain					355:360	The obtained U9 strain	339:360	The obtained U9 strain	339:360	The obtained U9 strain could produce 62.1 ± 1.2 g/l EPS while the V19 strain only produced 53.5 ± 1.3 g/l EPS.					
33091476	5	4	contain	had	994:996	arg1	U9-EPS					987:992	the purified bioflocculant U9-EPS	960:992	the purified bioflocculant U9-EPS	960:992	The results showed that the purified bioflocculant U9-EPS had effective flocculation of kaolin clay.					
33091476	5	4	contain	had	994:996	arg2	flocculation					1008:1019	effective flocculation	998:1019	effective flocculation of kaolin clay	998:1034	The results showed that the purified bioflocculant U9-EPS had effective flocculation of kaolin clay.					
33091476	5	5	theme	kaolin	1024:1029	arg1	clay					1031:1034	kaolin clay	1024:1034	kaolin clay	1024:1034	The results showed that the purified bioflocculant U9-EPS had effective flocculation of kaolin clay.					
33091476	5	6	theme	purified	964:971	arg1	U9-EPS					987:992	the purified bioflocculant U9-EPS	960:992	the purified bioflocculant U9-EPS	960:992	The results showed that the purified bioflocculant U9-EPS had effective flocculation of kaolin clay.					
33091476	6	7	theme	high	1057:1060	arg1	ability					1062:1068	high ability	1057:1068	high ability to flocculate the polluted river water and decolorize Congo red	1057:1132	The U9-EPS also had high ability to flocculate the polluted river water and decolorize Congo red.					
33091476	3	8	contain	contained	575:583	arg2	3.79:1:5.52					585:595	3.79:1:5.52	585:595	3.79:1:5.52	585:595	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	8	contain	contained	575:583	arg1	compositions					454:465	The compositions	450:465	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain	450:573	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	9	theme	glucuronic	496:505	arg1	acid					507:510	glucuronic acid	496:510	glucuronic acid	496:510	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	9	theme	glucuronic	496:505	arg1	monosaccharides					470:484	monosaccharides	470:484	monosaccharides (mannose, glucuronic acid and galactose)	470:525	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	10	theme	purified	534:541	arg1	EPS					543:545	the purified EPS	530:545	the purified EPS (U9-EPS) from the U9 strain	530:573	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	10	theme	purified	534:541	arg1	U9-EPS					548:553	U9-EPS	548:553	U9-EPS	548:553	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	5	11	theme	bioflocculant	973:985	arg1	U9-EPS					987:992	the purified bioflocculant U9-EPS	960:992	the purified bioflocculant U9-EPS	960:992	The results showed that the purified bioflocculant U9-EPS had effective flocculation of kaolin clay.					
33091476	1	12	theme	glucuronic	182:191	arg1	acid					193:196	glucuronic acid	182:196	glucuronic acid	182:196	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	0	13	theme	Improved	0:7	arg1	production					9:18	Improved production	0:18	Improved production of an acidic exopolysaccharide, the efficient flocculant, by Lipomyces starkeyi U9 overexpressing UDP-glucose dehydrogenase gene.	0:148	Improved production of an acidic exopolysaccharide, the efficient flocculant, by Lipomyces starkeyi U9 overexpressing UDP-glucose dehydrogenase gene.					
33091476	1	14	theme	UDP-glucose	263:273	arg1	dehydrogenase					275:287	UDP-glucose dehydrogenase	263:287	an UDP-glucose dehydrogenase gene	260:292	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	1	15	theme	acid	193:196	arg1	activity					250:257	its flocculating activity	233:257	its flocculating activity	233:257	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	1	15	theme	acid	193:196	arg1	content					171:177	content	171:177	content of glucuronic acid in the exopolysaccharide (EPS)	171:227	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	1	16	theme	dehydrogenase	275:287	arg1	gene					289:292	an UDP-glucose dehydrogenase gene	260:292	an UDP-glucose dehydrogenase gene	260:292	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	0	17	theme	dehydrogenase	130:142	arg1	gene					144:147	UDP-glucose dehydrogenase gene	118:147	UDP-glucose dehydrogenase gene	118:147	Improved production of an acidic exopolysaccharide, the efficient flocculant, by Lipomyces starkeyi U9 overexpressing UDP-glucose dehydrogenase gene.					
33091476	4	18	from	clay	703:706	arg1	rate					674:677	The flocculation rate	657:677	The flocculation rate of the U9-EPS on kaolin clay	657:706	The flocculation rate of the U9-EPS on kaolin clay reached 87.9%, which was significantly higher than that (74.7%) of the V19-EPS while the decolorization rate of Congo Red (CR) by the U9-EPS reached 94.3%, which was significantly higher than that of CR by the V19-EPS (86.23%).					
33091476	3	19	from	strain	568:573	arg1	EPS					543:545	the purified EPS	530:545	the purified EPS (U9-EPS) from the U9 strain	530:573	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	19	from	strain	568:573	arg1	U9-EPS					548:553	U9-EPS	548:553	U9-EPS	548:553	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	19	from	strain	568:573	arg1	compositions					454:465	The compositions	450:465	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain	450:573	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	20	theme	monosaccharides	470:484	arg1	compositions					454:465	The compositions	450:465	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain	450:573	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	2	21	theme	53.5 ± 1.3 g/l	430:443	arg1	EPS					445:447	53.5 ± 1.3 g/l EPS	430:447	53.5 ± 1.3 g/l EPS	430:447	The obtained U9 strain could produce 62.1 ± 1.2 g/l EPS while the V19 strain only produced 53.5 ± 1.3 g/l EPS.					
33091476	0	22	theme	UDP-glucose	118:128	arg1	dehydrogenase					130:142	UDP-glucose dehydrogenase	118:142	UDP-glucose dehydrogenase gene	118:147	Improved production of an acidic exopolysaccharide, the efficient flocculant, by Lipomyces starkeyi U9 overexpressing UDP-glucose dehydrogenase gene.					
33091476	0	23	theme	exopolysaccharide	33:49	arg1	production					9:18	Improved production	0:18	Improved production of an acidic exopolysaccharide, the efficient flocculant, by Lipomyces starkeyi U9 overexpressing UDP-glucose dehydrogenase gene.	0:148	Improved production of an acidic exopolysaccharide, the efficient flocculant, by Lipomyces starkeyi U9 overexpressing UDP-glucose dehydrogenase gene.					
33091476	2	24	theme	62.1 ± 1.2 g/l	376:389	arg1	EPS					391:393	62.1 ± 1.2 g/l EPS	376:393	62.1 ± 1.2 g/l EPS	376:393	The obtained U9 strain could produce 62.1 ± 1.2 g/l EPS while the V19 strain only produced 53.5 ± 1.3 g/l EPS.					
33091476	0	25	theme	acidic	26:31	arg1	exopolysaccharide					33:49	an acidic exopolysaccharide	23:49	an acidic exopolysaccharide	23:49	Improved production of an acidic exopolysaccharide, the efficient flocculant, by Lipomyces starkeyi U9 overexpressing UDP-glucose dehydrogenase gene.					
33091476	0	25	theme	acidic	26:31	arg1	flocculant					66:75	the efficient flocculant	52:75	the efficient flocculant	52:75	Improved production of an acidic exopolysaccharide, the efficient flocculant, by Lipomyces starkeyi U9 overexpressing UDP-glucose dehydrogenase gene.					
33091476	5	26	theme	clay	1031:1034	arg1	flocculation					1008:1019	effective flocculation	998:1019	effective flocculation of kaolin clay	998:1034	The results showed that the purified bioflocculant U9-EPS had effective flocculation of kaolin clay.					
33091476	1	27	from	activity	250:257	arg1	EPS					224:226	EPS	224:226	EPS	224:226	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	1	27	from	activity	250:257	arg1	exopolysaccharide					205:221	the exopolysaccharide	201:221	the exopolysaccharide (EPS)	201:227	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	3	28	theme	purified	616:623	arg1	V19-EPS					630:636	V19-EPS	630:636	V19-EPS	630:636	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	28	theme	purified	616:623	arg1	EPS					625:627	the purified EPS	612:627	the purified EPS (V19-EPS)	612:637	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	29	from	compositions	454:465	arg1	EPS					543:545	the purified EPS	530:545	the purified EPS (U9-EPS) from the U9 strain	530:573	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	29	from	compositions	454:465	arg1	strain					568:573	the U9 strain	561:573	the U9 strain	561:573	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	29	from	compositions	454:465	arg1	U9-EPS					548:553	U9-EPS	548:553	U9-EPS	548:553	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	2	30	theme	V19	405:407	arg1	strain					409:414	the V19 strain	401:414	the V19 strain	401:414	The obtained U9 strain could produce 62.1 ± 1.2 g/l EPS while the V19 strain only produced 53.5 ± 1.3 g/l EPS.					
33091476	3	31	theme	U9	565:566	arg1	strain					568:573	the U9 strain	561:573	the U9 strain	561:573	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	2	32	theme	obtained	343:350	arg1	strain					355:360	The obtained U9 strain	339:360	The obtained U9 strain	339:360	The obtained U9 strain could produce 62.1 ± 1.2 g/l EPS while the V19 strain only produced 53.5 ± 1.3 g/l EPS.					
33091476	1	33	theme	Lipomyces	315:323	arg1	V19					334:336	Lipomyces starkeyi V19	315:336	Lipomyces starkeyi V19	315:336	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	4	34	theme	Red	826:828	arg1	rate					812:815	the decolorization rate	793:815	the decolorization rate of Congo Red (CR) by the U9-EPS	793:847	The flocculation rate of the U9-EPS on kaolin clay reached 87.9%, which was significantly higher than that (74.7%) of the V19-EPS while the decolorization rate of Congo Red (CR) by the U9-EPS reached 94.3%, which was significantly higher than that of CR by the V19-EPS (86.23%).					
33091476	6	35	theme	river	1097:1101	arg1	water					1103:1107	the polluted river water	1084:1107	the polluted river water	1084:1107	The U9-EPS also had high ability to flocculate the polluted river water and decolorize Congo red.					
33091476	1	36	theme	starkeyi	325:332	arg1	V19					334:336	Lipomyces starkeyi V19	315:336	Lipomyces starkeyi V19	315:336	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	4	37	theme	U9-EPS	686:691	arg1	rate					674:677	The flocculation rate	657:677	The flocculation rate of the U9-EPS on kaolin clay	657:706	The flocculation rate of the U9-EPS on kaolin clay reached 87.9%, which was significantly higher than that (74.7%) of the V19-EPS while the decolorization rate of Congo Red (CR) by the U9-EPS reached 94.3%, which was significantly higher than that of CR by the V19-EPS (86.23%).					
33091476	6	38	theme	polluted	1088:1095	arg1	water					1103:1107	the polluted river water	1084:1107	the polluted river water	1084:1107	The U9-EPS also had high ability to flocculate the polluted river water and decolorize Congo red.					
33091476	4	39	theme	kaolin	696:701	arg1	clay					703:706	kaolin clay	696:706	kaolin clay	696:706	The flocculation rate of the U9-EPS on kaolin clay reached 87.9%, which was significantly higher than that (74.7%) of the V19-EPS while the decolorization rate of Congo Red (CR) by the U9-EPS reached 94.3%, which was significantly higher than that of CR by the V19-EPS (86.23%).					
33091476	4	40	from	U9-EPS	686:691	arg1	clay					703:706	kaolin clay	696:706	kaolin clay	696:706	The flocculation rate of the U9-EPS on kaolin clay reached 87.9%, which was significantly higher than that (74.7%) of the V19-EPS while the decolorization rate of Congo Red (CR) by the U9-EPS reached 94.3%, which was significantly higher than that of CR by the V19-EPS (86.23%).					
33091476	6	41	contain	had	1053:1055	arg2	ability					1062:1068	high ability	1057:1068	high ability to flocculate the polluted river water and decolorize Congo red	1057:1132	The U9-EPS also had high ability to flocculate the polluted river water and decolorize Congo red.					
33091476	6	41	contain	had	1053:1055	arg1	U9-EPS					1041:1046	The U9-EPS	1037:1046	The U9-EPS	1037:1046	The U9-EPS also had high ability to flocculate the polluted river water and decolorize Congo red.					
33091476	3	42	dep	monosaccharides	470:484	arg1	galactose					516:524	galactose	516:524	galactose	516:524	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	42	dep	monosaccharides	470:484	arg1	mannose					487:493	mannose	487:493	mannose	487:493	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	42	dep	monosaccharides	470:484	arg1	acid					507:510	glucuronic acid	496:510	glucuronic acid	496:510	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	3	42	dep	monosaccharides	470:484	arg1	monosaccharides					470:484	monosaccharides	470:484	monosaccharides (mannose, glucuronic acid and galactose)	470:525	The compositions of monosaccharides (mannose, glucuronic acid and galactose) in the purified EPS (U9-EPS) from the U9 strain contained 3.79:1:5.52 while those in the purified EPS (V19-EPS) were 3.94:1:6.29.					
33091476	4	43	theme	flocculation	661:672	arg1	rate					674:677	The flocculation rate	657:677	The flocculation rate of the U9-EPS on kaolin clay	657:706	The flocculation rate of the U9-EPS on kaolin clay reached 87.9%, which was significantly higher than that (74.7%) of the V19-EPS while the decolorization rate of Congo Red (CR) by the U9-EPS reached 94.3%, which was significantly higher than that of CR by the V19-EPS (86.23%).					
33091476	0	44	theme	efficient	56:64	arg1	exopolysaccharide					33:49	an acidic exopolysaccharide	23:49	an acidic exopolysaccharide	23:49	Improved production of an acidic exopolysaccharide, the efficient flocculant, by Lipomyces starkeyi U9 overexpressing UDP-glucose dehydrogenase gene.					
33091476	0	44	theme	efficient	56:64	arg1	flocculant					66:75	the efficient flocculant	52:75	the efficient flocculant	52:75	Improved production of an acidic exopolysaccharide, the efficient flocculant, by Lipomyces starkeyi U9 overexpressing UDP-glucose dehydrogenase gene.					
33091476	5	45	theme	effective	998:1006	arg1	flocculation					1008:1019	effective flocculation	998:1019	effective flocculation of kaolin clay	998:1034	The results showed that the purified bioflocculant U9-EPS had effective flocculation of kaolin clay.					
33091476	1	46	from	content	171:177	arg1	EPS					224:226	EPS	224:226	EPS	224:226	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
33091476	1	46	from	content	171:177	arg1	exopolysaccharide					205:221	the exopolysaccharide	201:221	the exopolysaccharide (EPS)	201:227	In order to increase content of glucuronic acid in the exopolysaccharide (EPS) and its flocculating activity, an UDP-glucose dehydrogenase gene was overexpressed in Lipomyces starkeyi V19.					
32016408	11	0	dep	pectins	1763:1769	arg1	the					1759:1761	the	1759:1761	the	1759:1761	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	7	1	theme	AZ	1192:1193	arg1	walls					1200:1204	the AZ cell walls	1188:1204	the AZ cell walls	1188:1204	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	7	1	theme	AZ	1192:1193	arg1	zones					1261:1265	tricellular junction zones	1240:1265	tricellular junction zones	1240:1265	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	7	1	theme	AZ	1192:1193	arg1	lamella					1228:1234	the middle lamella	1217:1234	the middle lamella	1217:1234	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	10	2	theme	AGPs	1644:1647	arg1	levels					1634:1639	lower levels	1628:1639	lower levels of AGPs during ripe fruit abscission	1628:1676	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	10	3	with	membrane	1588:1595	arg1	levels					1634:1639	lower levels	1628:1639	lower levels of AGPs during ripe fruit abscission	1628:1676	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	4	4	theme	pectins	589:595	arg1	localization					573:584	the localization	569:584	the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive	569:737	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	4	4	theme	pectins	589:595	arg1	wall					533:536	cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive	528:737	wall	533:536	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	2	5	theme	cell	284:287	arg1	composition					294:304	cell wall composition	284:304	cell wall composition	284:304	However, changes in cell wall composition during natural fruit abscission are poorly understood.					
32016408	10	6	with	cytoplasm	1601:1609	arg1	levels					1634:1639	lower levels	1628:1639	lower levels of AGPs during ripe fruit abscission	1628:1676	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	0	7	theme	Cell	0:3	arg1	Composition					10:20	Cell Wall Composition	0:20	Cell Wall Composition	0:20	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	5	8	theme	wall	824:827	arg1	components					829:838	cell wall components	819:838	cell wall components	819:838	To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ.					
32016408	11	9	theme	polysaccharide	1727:1740	arg1	immunolocalization					1779:1796	AGP immunolocalization	1775:1796	AGP immunolocalization	1775:1796	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	11	9	theme	polysaccharide	1727:1740	arg1	composition					1742:1752	the cell wall polysaccharide composition	1713:1752	the cell wall polysaccharide composition	1713:1752	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	11	9	theme	polysaccharide	1727:1740	arg1	pectins					1763:1769	pectins	1763:1769	pectins	1763:1769	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	2	10	theme	natural	313:319	arg1	abscission					327:336	natural fruit abscission	313:336	natural fruit abscission	313:336	However, changes in cell wall composition during natural fruit abscission are poorly understood.					
32016408	11	11	theme	cell	1717:1720	arg1	immunolocalization					1779:1796	AGP immunolocalization	1775:1796	AGP immunolocalization	1775:1796	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	11	11	theme	cell	1717:1720	arg1	composition					1742:1752	the cell wall polysaccharide composition	1713:1752	the cell wall polysaccharide composition	1713:1752	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	11	11	theme	cell	1717:1720	arg1	pectins					1763:1769	pectins	1763:1769	pectins	1763:1769	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	11	12	theme	olive	1805:1809	arg1	AZ					1817:1818	the olive fruit AZ	1801:1818	the olive fruit AZ	1801:1818	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	5	13	theme	monoclonal	794:803	arg1	antibodies					805:814	monoclonal antibodies	794:814	monoclonal antibodies to cell wall components	794:838	To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ.					
32016408	10	14	theme	cell	1570:1573	arg1	wall					1575:1578	the cell wall	1566:1578	the cell wall	1566:1578	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	11	15	theme	ripe	1874:1877	arg1	abscission					1885:1894	ripe fruit abscission	1874:1894	ripe fruit abscission	1874:1894	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	9	16	theme	galactose-rich	1441:1454	arg1	pectins					1475:1481	both galactose-rich and arabinose-rich pectins	1436:1481	both galactose-rich and arabinose-rich pectins	1436:1481	Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission.					
32016408	4	17	theme	abscission	702:711	arg1	control					713:719	fruit abscission control	696:719	fruit abscission control in 'Picual' olive	696:737	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	6	18	dep	de-esterification	1000:1016	arg1	the					996:998	the	996:998	the	996:998	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	9	19	theme	arabinose-rich	1460:1473	arg1	pectins					1475:1481	both galactose-rich and arabinose-rich pectins	1436:1481	both galactose-rich and arabinose-rich pectins	1436:1481	Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission.					
32016408	3	20	theme	natural	438:444	arg1	abscission					452:461	massive natural fruit abscission	430:461	massive natural fruit abscission	430:461	In olive (Olea europaea L.), some cultivars such as 'Picual' undergo massive natural fruit abscission after fruit ripening.					
32016408	7	21	theme	esterified	1117:1126	arg1	HGs					1164:1166	HGs	1164:1166	HGs	1164:1166	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	7	21	theme	esterified	1117:1126	arg1	homogalacturonans					1145:1161	both esterified and unesterified homogalacturonans	1112:1161	both esterified and unesterified homogalacturonans (HGs)	1112:1167	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	0	22	theme	Protein	91:97	arg1	Composition					10:20	Cell Wall Composition	0:20	Cell Wall Composition	0:20	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	0	22	theme	Protein	91:97	arg1	Immunolocalization					42:59	Ultrastructural Immunolocalization	26:59	Ultrastructural Immunolocalization	26:59	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	9	23	theme	cell	1489:1492	arg1	walls					1494:1498	AZ cell walls	1486:1498	AZ cell walls	1486:1498	Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission.					
32016408	6	24	theme	wall	914:917	arg1	extraction					934:943	Cell wall polysaccharide extraction	909:943	Cell wall polysaccharide extraction	909:943	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	8	25	theme	unesterified	1294:1305	arg1	HGs					1307:1309	unesterified HGs	1294:1309	unesterified HGs	1294:1309	Our results indicate that unesterified HGs are likely to contribute to cell separation in the olive fruit AZ.					
32016408	11	26	theme	temporal	1693:1700	arg1	profile					1702:1708	This detailed temporal profile	1679:1708	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ,	1679:1819	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	0	27	theme	Olea	106:109	arg1	Abscission					129:138	Olea europaea L. Fruit Abscission	106:138	Olea europaea L. Fruit Abscission	106:138	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	5	28	theme	olive	893:897	arg1	AZ					905:906	olive fruit AZ	893:906	olive fruit AZ	893:906	To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ.					
32016408	1	29	theme	wall	146:149	arg1	modification					151:162	Cell wall modification	141:162	Cell wall modification	141:162	Cell wall modification is integral to many plant developmental processes where cells need to separate, such as abscission.					
32016408	3	30	theme	fruit	446:450	arg1	abscission					452:461	massive natural fruit abscission	430:461	massive natural fruit abscission	430:461	In olive (Olea europaea L.), some cultivars such as 'Picual' undergo massive natural fruit abscission after fruit ripening.					
32016408	11	31	theme	new	1828:1830	arg1	insights					1832:1839	new insights	1828:1839	new insights into cell wall remodeling during ripe fruit abscission	1828:1894	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	0	32	theme	L.	120:121	arg1	Abscission					129:138	Olea europaea L. Fruit Abscission	106:138	Olea europaea L. Fruit Abscission	106:138	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	9	33	theme	fruit	1512:1516	arg1	abscission					1518:1527	ripe fruit abscission	1507:1527	ripe fruit abscission	1507:1527	Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission.					
32016408	11	34	theme	cell	1846:1849	arg1	remodeling					1856:1865	cell wall remodeling	1846:1865	cell wall remodeling during ripe fruit abscission	1846:1894	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	10	35	theme	plasma	1581:1586	arg1	membrane					1588:1595	plasma membrane	1581:1595	plasma membrane	1581:1595	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	7	36	theme	unesterified	1132:1143	arg1	HGs					1164:1166	HGs	1164:1166	HGs	1164:1166	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	7	36	theme	unesterified	1132:1143	arg1	homogalacturonans					1145:1161	both esterified and unesterified homogalacturonans	1112:1161	both esterified and unesterified homogalacturonans (HGs)	1112:1167	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	3	37	theme	Olea	371:374	arg1	L.					385:386	Olea europaea L.	371:386	Olea europaea L.	371:386	In olive (Olea europaea L.), some cultivars such as 'Picual' undergo massive natural fruit abscission after fruit ripening.					
32016408	3	37	theme	Olea	371:374	arg1	olive					364:368	olive	364:368	olive (Olea europaea L.)	364:387	In olive (Olea europaea L.), some cultivars such as 'Picual' undergo massive natural fruit abscission after fruit ripening.					
32016408	4	38	theme	Picual	725:730	arg1	olive					733:737	'Picual' olive	724:737	'Picual' olive	724:737	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	10	39	theme	cells	1617:1621	arg1	membrane					1588:1595	plasma membrane	1581:1595	plasma membrane	1581:1595	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	10	39	theme	cells	1617:1621	arg1	cytoplasm					1601:1609	cytoplasm	1601:1609	cytoplasm	1601:1609	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	10	39	theme	cells	1617:1621	arg1	wall					1575:1578	the cell wall	1566:1578	the cell wall	1566:1578	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	4	40	from	wall	533:536	arg1	zone					649:652	the abscission zone	634:652	the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive	634:737	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	4	40	from	wall	533:536	arg1	AZ					655:656	AZ	655:656	AZ	655:656	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	5	41	theme	cell	819:822	arg1	components					829:838	cell wall components	819:838	cell wall components	819:838	To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ.					
32016408	0	42	theme	Ultrastructural	26:40	arg1	Immunolocalization					42:59	Ultrastructural Immunolocalization	26:59	Ultrastructural Immunolocalization	26:59	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	9	43	from	decrease	1424:1431	arg1	walls					1494:1498	AZ cell walls	1486:1498	AZ cell walls	1486:1498	Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission.					
32016408	9	43	from	decrease	1424:1431	arg1	pectins					1475:1481	both galactose-rich and arabinose-rich pectins	1436:1481	both galactose-rich and arabinose-rich pectins	1436:1481	Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission.					
32016408	7	44	theme	tricellular	1240:1250	arg1	zones					1261:1265	tricellular junction zones	1240:1265	tricellular junction zones	1240:1265	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	3	45	theme	fruit	469:473	arg1	ripening					475:482	fruit ripening	469:482	fruit ripening	469:482	In olive (Olea europaea L.), some cultivars such as 'Picual' undergo massive natural fruit abscission after fruit ripening.					
32016408	5	46	theme	immunogold	753:762	arg1	labeling					764:771	immunogold labeling	753:771	immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20)	753:871	To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ.					
32016408	11	47	theme	wall	1851:1854	arg1	remodeling					1856:1865	cell wall remodeling	1846:1865	cell wall remodeling during ripe fruit abscission	1846:1894	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	8	48	theme	fruit	1368:1372	arg1	AZ					1374:1375	the olive fruit AZ	1358:1375	the olive fruit AZ	1358:1375	Our results indicate that unesterified HGs are likely to contribute to cell separation in the olive fruit AZ.					
32016408	10	49	theme	lower	1628:1632	arg1	levels					1634:1639	lower levels	1628:1639	lower levels of AGPs during ripe fruit abscission	1628:1676	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	6	50	theme	polysaccharides	1044:1058	arg1	degradation					1022:1032	degradation	1022:1032	degradation	1022:1032	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	6	50	theme	polysaccharides	1044:1058	arg1	de-esterification					1000:1016	de-esterification	1000:1016	de-esterification	1000:1016	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	6	51	theme	AZ	963:964	arg1	separation					971:980	the AZ cell separation	959:980	the AZ cell separation	959:980	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	6	51	theme	AZ	963:964	arg1	related					985:991	related	985:991	related	985:991	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	10	52	theme	fruit	1661:1665	arg1	abscission					1667:1676	ripe fruit abscission	1656:1676	ripe fruit abscission	1656:1676	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	1	53	theme	plant	184:188	arg1	processes					204:212	many plant developmental processes	179:212	many plant developmental processes	179:212	Cell wall modification is integral to many plant developmental processes where cells need to separate, such as abscission.					
32016408	1	53	theme	plant	184:188	arg1	abscission					252:261	abscission	252:261	abscission	252:261	Cell wall modification is integral to many plant developmental processes where cells need to separate, such as abscission.					
32016408	0	54	theme	Pectin	64:69	arg1	Composition					10:20	Cell Wall Composition	0:20	Cell Wall Composition	0:20	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	0	54	theme	Pectin	64:69	arg1	Immunolocalization					42:59	Ultrastructural Immunolocalization	26:59	Ultrastructural Immunolocalization	26:59	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	10	55	with	wall	1575:1578	arg1	levels					1634:1639	lower levels	1628:1639	lower levels of AGPs during ripe fruit abscission	1628:1676	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	4	56	theme	protein	617:623	arg1	localization					573:584	the localization	569:584	the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive	569:737	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	4	56	theme	protein	617:623	arg1	wall					533:536	cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive	528:737	wall	533:536	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	5	57	dep	suite	785:789	arg1	LM20					867:870	LM20	867:870	LM20	867:870	To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ.					
32016408	5	57	dep	suite	785:789	arg1	LM19					858:861	LM19	858:861	LM19	858:861	To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ.					
32016408	7	58	theme	cell	1195:1198	arg1	walls					1200:1204	the AZ cell walls	1188:1204	the AZ cell walls	1188:1204	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	7	58	theme	cell	1195:1198	arg1	zones					1261:1265	tricellular junction zones	1240:1265	tricellular junction zones	1240:1265	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	7	58	theme	cell	1195:1198	arg1	lamella					1228:1234	the middle lamella	1217:1234	the middle lamella	1217:1234	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	2	59	theme	wall	289:292	arg1	composition					294:304	cell wall composition	284:304	cell wall composition	284:304	However, changes in cell wall composition during natural fruit abscission are poorly understood.					
32016408	11	60	from	pectins	1763:1769	arg1	AZ					1817:1818	the olive fruit AZ	1801:1818	the olive fruit AZ	1801:1818	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	1	61	theme	developmental	190:202	arg1	processes					204:212	many plant developmental processes	179:212	many plant developmental processes	179:212	Cell wall modification is integral to many plant developmental processes where cells need to separate, such as abscission.					
32016408	1	61	theme	developmental	190:202	arg1	abscission					252:261	abscission	252:261	abscission	252:261	Cell wall modification is integral to many plant developmental processes where cells need to separate, such as abscission.					
32016408	1	62	theme	many	179:182	arg1	processes					204:212	many plant developmental processes	179:212	many plant developmental processes	179:212	Cell wall modification is integral to many plant developmental processes where cells need to separate, such as abscission.					
32016408	1	62	theme	many	179:182	arg1	abscission					252:261	abscission	252:261	abscission	252:261	Cell wall modification is integral to many plant developmental processes where cells need to separate, such as abscission.					
32016408	4	63	theme	abscission	638:647	arg1	zone					649:652	the abscission zone	634:652	the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive	634:737	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	4	63	theme	abscission	638:647	arg1	AZ					655:656	AZ	655:656	AZ	655:656	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	2	64	theme	fruit	321:325	arg1	abscission					327:336	natural fruit abscission	313:336	natural fruit abscission	313:336	However, changes in cell wall composition during natural fruit abscission are poorly understood.					
32016408	9	65	theme	immunogold	1389:1398	arg1	labeling					1400:1407	immunogold labeling	1389:1407	immunogold labeling	1389:1407	Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission.					
32016408	11	66	theme	composition	1742:1752	arg1	profile					1702:1708	This detailed temporal profile	1679:1708	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ,	1679:1819	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	11	67	theme	wall	1722:1725	arg1	immunolocalization					1779:1796	AGP immunolocalization	1775:1796	AGP immunolocalization	1775:1796	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	11	67	theme	wall	1722:1725	arg1	composition					1742:1752	the cell wall polysaccharide composition	1713:1752	the cell wall polysaccharide composition	1713:1752	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	11	67	theme	wall	1722:1725	arg1	pectins					1763:1769	pectins	1763:1769	pectins	1763:1769	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	11	68	theme	fruit	1879:1883	arg1	abscission					1885:1894	ripe fruit abscission	1874:1894	ripe fruit abscission	1874:1894	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	5	69	theme	antibodies	805:814	arg1	suite					785:789	a suite	783:789	a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20)	783:871	To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ.					
32016408	1	70	theme	Cell	141:144	arg1	modification					151:162	Cell wall modification	141:162	Cell wall modification	141:162	Cell wall modification is integral to many plant developmental processes where cells need to separate, such as abscission.					
32016408	2	71	from	changes	273:279	arg1	composition					294:304	cell wall composition	284:304	cell wall composition	284:304	However, changes in cell wall composition during natural fruit abscission are poorly understood.					
32016408	4	72	theme	fruit	696:700	arg1	control					713:719	fruit abscission control	696:719	fruit abscission control in 'Picual' olive	696:737	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	11	73	theme	detailed	1684:1691	arg1	profile					1702:1708	This detailed temporal profile	1679:1708	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ,	1679:1819	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	0	74	theme	Arabinogalactan	75:89	arg1	Protein					91:97	Arabinogalactan Protein	75:97	Arabinogalactan Protein	75:97	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	9	75	theme	AZ	1486:1487	arg1	walls					1494:1498	AZ cell walls	1486:1498	AZ cell walls	1486:1498	Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission.					
32016408	6	76	theme	polysaccharide	919:932	arg1	extraction					934:943	Cell wall polysaccharide extraction	909:943	Cell wall polysaccharide extraction	909:943	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	4	77	theme	cell	666:669	arg1	separation					671:680	cell separation	666:680	cell separation	666:680	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	0	78	theme	europaea	111:118	arg1	Abscission					129:138	Olea europaea L. Fruit Abscission	106:138	Olea europaea L. Fruit Abscission	106:138	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	8	79	theme	olive	1362:1366	arg1	AZ					1374:1375	the olive fruit AZ	1358:1375	the olive fruit AZ	1358:1375	Our results indicate that unesterified HGs are likely to contribute to cell separation in the olive fruit AZ.					
32016408	6	80	theme	Cell	909:912	arg1	extraction					934:943	Cell wall polysaccharide extraction	909:943	Cell wall polysaccharide extraction	909:943	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	0	81	theme	Fruit	123:127	arg1	Abscission					129:138	Olea europaea L. Fruit Abscission	106:138	Olea europaea L. Fruit Abscission	106:138	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	5	82	theme	fruit	899:903	arg1	AZ					905:906	olive fruit AZ	893:906	olive fruit AZ	893:906	To this end, immunogold labeling employing a suite of monoclonal antibodies to cell wall components (JIM13, LM5, LM6, LM19 and LM20) was investigated in olive fruit AZ.					
32016408	4	83	from	localization	573:584	arg1	zone					649:652	the abscission zone	634:652	the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive	634:737	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	4	83	from	localization	573:584	arg1	AZ					655:656	AZ	655:656	AZ	655:656	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	0	84	theme	Wall	5:8	arg1	Composition					10:20	Cell Wall Composition	0:20	Cell Wall Composition	0:20	Cell Wall Composition and Ultrastructural Immunolocalization of Pectin and Arabinogalactan Protein during Olea europaea L. Fruit Abscission.					
32016408	4	85	dep	wall	533:536	arg1	composition					553:563	polysaccharide composition	538:563	polysaccharide composition	538:563	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	11	86	theme	fruit	1811:1815	arg1	AZ					1817:1818	the olive fruit AZ	1801:1818	the olive fruit AZ	1801:1818	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	4	87	theme	polysaccharide	538:551	arg1	composition					553:563	polysaccharide composition	538:563	polysaccharide composition	538:563	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	11	88	from	immunolocalization	1779:1796	arg1	AZ					1817:1818	the olive fruit AZ	1801:1818	the olive fruit AZ	1801:1818	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	4	89	from	control	713:719	arg1	olive					733:737	'Picual' olive	724:737	'Picual' olive	724:737	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	4	90	from	differences	513:523	arg1	localization					573:584	the localization	569:584	the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive	569:737	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	4	90	from	differences	513:523	arg1	wall					533:536	cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive	528:737	wall	533:536	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	7	91	theme	junction	1252:1259	arg1	zones					1261:1265	tricellular junction zones	1240:1265	tricellular junction zones	1240:1265	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	3	92	theme	europaea	376:383	arg1	L.					385:386	Olea europaea L.	371:386	Olea europaea L.	371:386	In olive (Olea europaea L.), some cultivars such as 'Picual' undergo massive natural fruit abscission after fruit ripening.					
32016408	3	92	theme	europaea	376:383	arg1	olive					364:368	olive	364:368	olive (Olea europaea L.)	364:387	In olive (Olea europaea L.), some cultivars such as 'Picual' undergo massive natural fruit abscission after fruit ripening.					
32016408	9	93	theme	ripe	1507:1510	arg1	abscission					1518:1527	ripe fruit abscission	1507:1527	ripe fruit abscission	1507:1527	Similarly, immunogold labeling demonstrated a decrease in both galactose-rich and arabinose-rich pectins in AZ cell walls during ripe fruit abscission.					
32016408	10	94	theme	AZ	1614:1615	arg1	cells					1617:1621	AZ cells	1614:1621	AZ cells	1614:1621	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	6	95	theme	cell	966:969	arg1	separation					971:980	the AZ cell separation	959:980	the AZ cell separation	959:980	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	6	95	theme	cell	966:969	arg1	related					985:991	related	985:991	related	985:991	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	8	96	theme	cell	1339:1342	arg1	separation					1344:1353	cell separation	1339:1353	cell separation	1339:1353	Our results indicate that unesterified HGs are likely to contribute to cell separation in the olive fruit AZ.					
32016408	3	97	theme	massive	430:436	arg1	abscission					452:461	massive natural fruit abscission	430:461	massive natural fruit abscission	430:461	In olive (Olea europaea L.), some cultivars such as 'Picual' undergo massive natural fruit abscission after fruit ripening.					
32016408	7	98	theme	ultrastructural	1071:1085	arg1	localization					1087:1098	ultrastructural localization	1071:1098	ultrastructural localization	1071:1098	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	7	99	theme	middle	1221:1226	arg1	lamella					1228:1234	the middle lamella	1217:1234	the middle lamella	1217:1234	Moreover, ultrastructural localization showed that both esterified and unesterified homogalacturonans (HGs) localize mainly in the AZ cell walls, including the middle lamella and tricellular junction zones.					
32016408	4	100	theme	cell	528:531	arg1	wall					533:536	cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive	528:737	wall	533:536	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	4	101	theme	arabinogalactan	601:615	arg1	AGP					626:628	AGP	626:628	AGP	626:628	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	4	101	theme	arabinogalactan	601:615	arg1	protein					617:623	arabinogalactan protein	601:623	arabinogalactan protein (AGP)	601:629	This study investigates the differences in cell wall polysaccharide composition and the localization of pectins and arabinogalactan protein (AGP) in the abscission zone (AZ) during cell separation to understand fruit abscission control in 'Picual' olive.					
32016408	10	102	located	localized	1553:1561	arg1	membrane					1588:1595	plasma membrane	1581:1595	plasma membrane	1581:1595	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	10	102	located	localized	1553:1561	arg1	wall					1575:1578	the cell wall	1566:1578	the cell wall	1566:1578	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	10	102	located	localized	1553:1561	arg1	cytoplasm					1601:1609	cytoplasm	1601:1609	cytoplasm	1601:1609	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	10	102	located	localized	1553:1561	arg2	AGPs					1543:1546	AGPs	1543:1546	AGPs	1543:1546	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	10	102	located	localized	1553:1561	arg1	addition					1533:1540	addition	1533:1540	addition	1533:1540	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
32016408	11	103	theme	AGP	1775:1777	arg1	immunolocalization					1779:1796	AGP immunolocalization	1775:1796	AGP immunolocalization	1775:1796	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	11	103	theme	AGP	1775:1777	arg1	composition					1742:1752	the cell wall polysaccharide composition	1713:1752	the cell wall polysaccharide composition	1713:1752	This detailed temporal profile of the cell wall polysaccharide composition, and the pectins and AGP immunolocalization in the olive fruit AZ, offers new insights into cell wall remodeling during ripe fruit abscission.					
32016408	6	104	theme	pectic	1037:1042	arg1	polysaccharides					1044:1058	pectic polysaccharides	1037:1058	pectic polysaccharides	1037:1058	Cell wall polysaccharide extraction revealed that the AZ cell separation is related to the de-esterification and degradation of pectic polysaccharides.					
32016408	10	105	theme	ripe	1656:1659	arg1	abscission					1667:1676	ripe fruit abscission	1656:1676	ripe fruit abscission	1656:1676	In addition, AGPs were localized in the cell wall, plasma membrane and cytoplasm of AZ cells with lower levels of AGPs during ripe fruit abscission.					
33805791	6	0	theme	residues	1395:1402	arg1	sugar					1374:1378	neutral sugar and polyphenol residues	1366:1402	sugar	1374:1378	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	6	0	theme	residues	1395:1402	arg1	residues					1395:1402	neutral sugar and polyphenol residues	1366:1402	residues	1395:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	6	0	theme	residues	1395:1402	arg1	GalA					1340:1343	GalA	1340:1343	GalA	1340:1343	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	6	0	theme	residues	1395:1402	arg1	amounts					1355:1361	small amounts	1349:1361	small amounts of neutral sugar and polyphenol residues	1349:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	5	1	theme	persimmon	1133:1141	arg1	effects					1122:1128	the beneficial effects	1107:1128	the beneficial effects of persimmon	1107:1141	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	6	2	theme	kDa	1314:1316	arg1	weight					1299:1304	a molecular weight	1287:1304	a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues	1287:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	2	3	theme	high-molecular-weight	366:386	arg1	compounds					388:396	its high-molecular-weight compounds	362:396	its high-molecular-weight compounds	362:396	However, its high-molecular-weight compounds, particularly polysaccharides, have not been extensively studied.					
33805791	2	3	theme	high-molecular-weight	366:386	arg1	polysaccharides					412:426	polysaccharides	412:426	particularly polysaccharides	399:426	However, its high-molecular-weight compounds, particularly polysaccharides, have not been extensively studied.					
33805791	1	4	theme	several	238:244	arg1	effects					260:266	several physiological effects	238:266	several physiological effects	238:266	Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals.					
33805791	6	5	theme	polyphenol	1384:1393	arg1	residues					1395:1402	neutral sugar and polyphenol residues	1366:1402	residues	1395:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	7	6	theme	GalA-rich	1478:1486	arg1	fraction					1446:1453	the bioactive fraction DK-H	1432:1458	the bioactive fraction DK-H	1432:1458	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	6	theme	GalA-rich	1478:1486	arg1	likely					1463:1468	likely	1463:1468	likely	1463:1468	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	6	theme	GalA-rich	1478:1486	arg1	polysaccharide					1495:1508	a GalA-rich pectic polysaccharide	1476:1508	a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries	1476:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	1	7	theme	physiological	246:258	arg1	effects					260:266	several physiological effects	238:266	several physiological effects	238:266	Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals.					
33805791	7	8	theme	pectic	1488:1493	arg1	fraction					1446:1453	the bioactive fraction DK-H	1432:1458	the bioactive fraction DK-H	1432:1458	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	8	theme	pectic	1488:1493	arg1	likely					1463:1468	likely	1463:1468	likely	1463:1468	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	8	theme	pectic	1488:1493	arg1	polysaccharide					1495:1508	a GalA-rich pectic polysaccharide	1476:1508	a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries	1476:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	5	9	theme	antiwrinkle	985:995	arg1	properties					997:1006	antiwrinkle properties	985:1006	antiwrinkle properties	985:1006	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	6	10	theme	sugar	1374:1378	arg1	sugar					1374:1378	neutral sugar and polyphenol residues	1366:1402	sugar	1374:1378	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	6	10	theme	sugar	1374:1378	arg1	residues					1395:1402	neutral sugar and polyphenol residues	1366:1402	residues	1395:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	6	10	theme	sugar	1374:1378	arg1	GalA					1340:1343	GalA	1340:1343	GalA	1340:1343	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	6	10	theme	sugar	1374:1378	arg1	amounts					1355:1361	small amounts	1349:1361	small amounts of neutral sugar and polyphenol residues	1349:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	6	11	theme	molecular	1289:1297	arg1	weight					1299:1304	a molecular weight	1287:1304	a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues	1287:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	5	12	theme	pharmacological	913:927	arg1	properties					997:1006	antiwrinkle properties	985:1006	antiwrinkle properties	985:1006	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	5	12	theme	pharmacological	913:927	arg1	activities					929:938	significantly improved pharmacological activities	890:938	significantly improved pharmacological activities	890:938	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	5	12	theme	pharmacological	913:927	arg1	anti-inflammatory					962:978	anti-inflammatory	962:978	anti-inflammatory	962:978	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	5	12	theme	pharmacological	913:927	arg1	antioxidant					949:959	antioxidant	949:959	antioxidant	949:959	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	6	13	theme	neutral	1366:1372	arg1	sugar					1374:1378	neutral sugar and polyphenol residues	1366:1402	sugar	1374:1378	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	3	14	theme	high-molecular-weight	531:551	arg1	DK-H					573:576	DK-H	573:576	DK-H	573:576	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	14	theme	high-molecular-weight	531:551	arg1	DK-L					564:567	DK-L	564:567	DK-L	564:567	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	14	theme	high-molecular-weight	531:551	arg1	fractions					553:561	low- and high-molecular-weight fractions	522:561	low- and high-molecular-weight fractions (DK-L and DK-H, respectively)	522:591	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	15	theme	in	728:729	arg1	system					737:742	an in vitro system	725:742	an in vitro system	725:742	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	7	16	contain	containing	1510:1519	arg1	fraction					1446:1453	the bioactive fraction DK-H	1432:1458	the bioactive fraction DK-H	1432:1458	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	16	contain	containing	1510:1519	arg1	likely					1463:1468	likely	1463:1468	likely	1463:1468	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	16	contain	containing	1510:1519	arg1	polysaccharide					1495:1508	a GalA-rich pectic polysaccharide	1476:1508	a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries	1476:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	16	contain	containing	1510:1519	arg2	candidate					1581:1589	a novel candidate	1573:1589	a novel candidate in the pharmaceutical and cosmeceutical industries	1573:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	16	contain	containing	1510:1519	arg2	number					1529:1534	a small number	1521:1534	a small number	1521:1534	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	4	17	theme	neutral	793:799	arg1	sugar					801:805	neutral sugar	793:805	neutral sugar	793:805	DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L.					
33805791	6	18	theme	molecular	1175:1183	arg1	characterization					1185:1200	molecular characterization	1175:1200	molecular characterization	1175:1200	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	3	19	from	effects	634:640	arg1	anti-inflammatory					658:674	anti-inflammatory	658:674	anti-inflammatory	658:674	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	19	from	effects	634:640	arg1	antioxidant					645:655	antioxidant	645:655	antioxidant	645:655	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	19	from	effects	634:640	arg1	activities					693:702	antiwrinkle activities	681:702	antiwrinkle activities	681:702	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	1	20	theme	familiar	177:184	arg1	Persimmon					147:155	Persimmon	147:155	Persimmon (Diospyros kaki)	147:172	Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals.					
33805791	1	20	theme	familiar	177:184	arg1	worldwide					207:215	a familiar and widespread fruit worldwide	175:215	a familiar and widespread fruit worldwide	175:215	Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals.					
33805791	6	21	theme	-rich	1261:1265	arg1	polysaccharide					1267:1280	a galacturonic acid (GalA)-rich polysaccharide	1235:1280	a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues	1235:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	6	22	theme	small	1349:1353	arg1	sugar					1374:1378	neutral sugar and polyphenol residues	1366:1402	sugar	1374:1378	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	6	22	theme	small	1349:1353	arg1	residues					1395:1402	neutral sugar and polyphenol residues	1366:1402	residues	1395:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	6	22	theme	small	1349:1353	arg1	amounts					1355:1361	small amounts	1349:1361	small amounts of neutral sugar and polyphenol residues	1349:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	0	23	theme	High-Molecular-Weight	13:33	arg1	Polysaccharides					35:49	a High-Molecular-Weight Polysaccharides	11:49	a High-Molecular-Weight Polysaccharides	11:49	Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity.					
33805791	4	24	theme	higher	774:779	arg1	contents					781:788	significantly higher contents	760:788	significantly higher contents of neutral sugar, uronic acid, and polyphenols	760:835	DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L.					
33805791	4	25	theme	polyphenols	825:835	arg1	contents					781:788	significantly higher contents	760:788	significantly higher contents of neutral sugar, uronic acid, and polyphenols	760:835	DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L.					
33805791	1	26	theme	widespread	190:199	arg1	Persimmon					147:155	Persimmon	147:155	Persimmon (Diospyros kaki)	147:172	Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals.					
33805791	1	26	theme	widespread	190:199	arg1	worldwide					207:215	a familiar and widespread fruit worldwide	175:215	a familiar and widespread fruit worldwide	175:215	Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals.					
33805791	0	27	theme	Antiwrinkle	125:135	arg1	Activity					137:144	Antiwrinkle Activity	125:144	Antiwrinkle Activity	125:144	Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity.					
33805791	6	28	theme	composition	1150:1160	arg1	analysis					1162:1169	Sugar composition analysis	1144:1169	Sugar composition analysis	1144:1169	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	1	29	theme	fruit	201:205	arg1	Persimmon					147:155	Persimmon	147:155	Persimmon (Diospyros kaki)	147:172	Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals.					
33805791	1	29	theme	fruit	201:205	arg1	worldwide					207:215	a familiar and widespread fruit worldwide	175:215	a familiar and widespread fruit worldwide	175:215	Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals.					
33805791	4	30	contain	contained	750:758	arg1	DK-H					745:748	DK-H	745:748	DK-H	745:748	DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L.					
33805791	4	30	contain	contained	750:758	arg2	contents					781:788	significantly higher contents	760:788	significantly higher contents of neutral sugar, uronic acid, and polyphenols	760:835	DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L.					
33805791	5	31	theme	important	1079:1087	arg1	role					1089:1092	an important role	1076:1092	an important role	1076:1092	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	3	32	theme	ethanol	601:607	arg1	fractionation					609:621	ethanol fractionation	601:621	ethanol fractionation	601:621	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	0	33	theme	Polysaccharides	35:49	arg1	Effects					0:6	Effects	0:6	Effects of a High-Molecular-Weight Polysaccharides	0:49	Effects of a High-Molecular-Weight Polysaccharides Isolated from Korean Persimmon on the Antioxidant, Anti-Inflammatory, and Antiwrinkle Activity.					
33805791	3	34	dep	in	728:729	arg1	vitro					731:735	vitro	731:735	vitro	731:735	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	7	35	theme	novel	1575:1579	arg1	number					1529:1534	a small number	1521:1534	a small number	1521:1534	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	35	theme	novel	1575:1579	arg1	candidate					1581:1589	a novel candidate	1573:1589	a novel candidate in the pharmaceutical and cosmeceutical industries	1573:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	36	theme	polyphenol	1539:1548	arg1	residues					1550:1557	polyphenol residues	1539:1557	polyphenol residues	1539:1557	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	3	37	theme	D.	479:480	arg1	DK					496:497	DK	496:497	DK	496:497	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	37	theme	D.	479:480	arg1	extract					487:493	D. kaki extract	479:493	D. kaki extract (DK)	479:498	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	1	38	theme	active	313:318	arg1	compounds					320:328	pharmacologically active compounds	295:328	pharmacologically active compounds called phytochemicals	295:350	Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals.					
33805791	7	39	from	candidate	1581:1589	arg1	industries					1631:1640	the pharmaceutical and cosmeceutical industries	1594:1640	the pharmaceutical and cosmeceutical industries	1594:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	40	theme	residues	1550:1557	arg1	number					1529:1534	a small number	1521:1534	a small number	1521:1534	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	40	theme	residues	1550:1557	arg1	candidate					1581:1589	a novel candidate	1573:1589	a novel candidate in the pharmaceutical and cosmeceutical industries	1573:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	5	41	theme	beneficial	1111:1120	arg1	effects					1122:1128	the beneficial effects	1107:1128	the beneficial effects of persimmon	1107:1141	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	6	42	theme	Sugar	1144:1148	arg1	analysis					1162:1169	Sugar composition analysis	1144:1169	Sugar composition analysis	1144:1169	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	1	43	theme	compounds	320:328	arg1	presence					283:290	the presence	279:290	the presence of pharmacologically active compounds called phytochemicals	279:350	Persimmon (Diospyros kaki), a familiar and widespread fruit worldwide, is known to exhibit several physiological effects because of the presence of pharmacologically active compounds called phytochemicals.					
33805791	3	44	theme	antiwrinkle	681:691	arg1	activities					693:702	antiwrinkle activities	681:702	antiwrinkle activities	681:702	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	45	dep	fractions	553:561	arg1	DK-H					573:576	DK-H	573:576	DK-H	573:576	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	45	dep	fractions	553:561	arg1	DK-L					564:567	DK-L	564:567	DK-L	564:567	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	45	dep	fractions	553:561	arg1	fractions					553:561	low- and high-molecular-weight fractions	522:561	low- and high-molecular-weight fractions (DK-L and DK-H, respectively)	522:591	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	7	46	theme	cosmeceutical	1617:1629	arg1	industries					1631:1640	the pharmaceutical and cosmeceutical industries	1594:1640	the pharmaceutical and cosmeceutical industries	1594:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	6	47	theme	galacturonic	1237:1248	arg1	acid					1250:1253	galacturonic acid	1237:1253	galacturonic acid	1237:1253	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	4	48	theme	uronic	808:813	arg1	acid					815:818	uronic acid	808:818	uronic acid	808:818	DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L.					
33805791	7	49	theme	bioactive	1436:1444	arg1	fraction					1446:1453	the bioactive fraction DK-H	1432:1458	the bioactive fraction DK-H	1432:1458	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	49	theme	bioactive	1436:1444	arg1	likely					1463:1468	likely	1463:1468	likely	1463:1468	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	49	theme	bioactive	1436:1444	arg1	polysaccharide					1495:1508	a GalA-rich pectic polysaccharide	1476:1508	a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries	1476:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	4	50	theme	sugar	801:805	arg1	contents					781:788	significantly higher contents	760:788	significantly higher contents of neutral sugar, uronic acid, and polyphenols	760:835	DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L.					
33805791	7	51	theme	DK-H	1455:1458	arg1	fraction					1446:1453	the bioactive fraction DK-H	1432:1458	the bioactive fraction DK-H	1432:1458	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	51	theme	DK-H	1455:1458	arg1	likely					1463:1468	likely	1463:1468	likely	1463:1468	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	51	theme	DK-H	1455:1458	arg1	polysaccharide					1495:1508	a GalA-rich pectic polysaccharide	1476:1508	a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries	1476:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	52	theme	pharmaceutical	1598:1611	arg1	industries					1631:1640	the pharmaceutical and cosmeceutical industries	1594:1640	the pharmaceutical and cosmeceutical industries	1594:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	53	theme	small	1523:1527	arg1	number					1529:1534	a small number	1521:1534	a small number	1521:1534	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	7	53	theme	small	1523:1527	arg1	candidate					1581:1589	a novel candidate	1573:1589	a novel candidate in the pharmaceutical and cosmeceutical industries	1573:1640	These results suggest that the bioactive fraction DK-H is likely to be a GalA-rich pectic polysaccharide containing a small number of polyphenol residues, which may be a novel candidate in the pharmaceutical and cosmeceutical industries.					
33805791	3	54	theme	kaki	482:485	arg1	DK					496:497	DK	496:497	DK	496:497	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	54	theme	kaki	482:485	arg1	extract					487:493	D. kaki extract	479:493	D. kaki extract (DK)	479:498	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	55	theme	low-	522:525	arg1	DK-H					573:576	DK-H	573:576	DK-H	573:576	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	55	theme	low-	522:525	arg1	DK-L					564:567	DK-L	564:567	DK-L	564:567	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	3	55	theme	low-	522:525	arg1	fractions					553:561	low- and high-molecular-weight fractions	522:561	low- and high-molecular-weight fractions (DK-L and DK-H, respectively)	522:591	In this study, D. kaki extract (DK) was fractionated into low- and high-molecular-weight fractions (DK-L and DK-H, respectively) through ethanol fractionation, and their effects on antioxidant, anti-inflammatory, and antiwrinkle activities were investigated by an in vitro system.					
33805791	6	56	dep	-rich	1261:1265	arg1	acid					1250:1253	galacturonic acid	1237:1253	galacturonic acid	1237:1253	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33805791	4	57	theme	acid	815:818	arg1	contents					781:788	significantly higher contents	760:788	significantly higher contents of neutral sugar, uronic acid, and polyphenols	760:835	DK-H contained significantly higher contents of neutral sugar, uronic acid, and polyphenols compared to DK and DK-L.					
33805791	5	58	theme	improved	904:911	arg1	properties					997:1006	antiwrinkle properties	985:1006	antiwrinkle properties	985:1006	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	5	58	theme	improved	904:911	arg1	activities					929:938	significantly improved pharmacological activities	890:938	significantly improved pharmacological activities	890:938	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	5	58	theme	improved	904:911	arg1	anti-inflammatory					962:978	anti-inflammatory	962:978	anti-inflammatory	962:978	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	5	58	theme	improved	904:911	arg1	antioxidant					949:959	antioxidant	949:959	antioxidant	949:959	Furthermore, DK-H exhibited significantly improved pharmacological activities, such as antioxidant, anti-inflammatory, and antiwrinkle properties, compared to those of DK and DK-L, demonstrating that DK-H may play an important role in mediating the beneficial effects of persimmon.					
33805791	6	59	with	polysaccharide	1267:1280	arg1	weight					1299:1304	a molecular weight	1287:1304	a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues	1287:1402	Sugar composition analysis and molecular characterization indicated that DK-H consisted of a galacturonic acid (GalA)-rich polysaccharide with a molecular weight of >345 kDa that mainly comprised GalA and small amounts of neutral sugar and polyphenol residues.					
33892066	8	0	with	mannose	1321:1327	arg1	ratio					1362:1366	the ratio	1358:1366	the ratio of 2.9 : 6.6 : 166 : 2.6	1358:1391	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	1	theme	OSP	1259:1261	arg1	weight					1249:1254	The average molecular weight	1227:1254	RESULTS The average molecular weight of OSP	1219:1261	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	6	2	theme	column	968:973	arg1	chromatography					975:988	The DEAE-Sephadex-A25 cellulose column and Sephadex-G100 gel column chromatography	907:988	chromatography	975:988	The DEAE-Sephadex-A25 cellulose column and Sephadex-G100 gel column chromatography were employed to purify and character the intracellular OSP.					
33892066	11	3	theme	NO	1939:1940	arg1	regulation					1925:1934	the regulation	1921:1934	the regulation of NO and cytokines	1921:1954	Western blotting analysis showed that the regulation of NO and cytokines was mediated through mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways.					
33892066	6	4	theme	Sephadex-G100	950:962	arg1	chromatography					975:988	The DEAE-Sephadex-A25 cellulose column and Sephadex-G100 gel column chromatography	907:988	chromatography	975:988	The DEAE-Sephadex-A25 cellulose column and Sephadex-G100 gel column chromatography were employed to purify and character the intracellular OSP.					
33892066	10	5	theme	chain	1624:1628	arg1	reaction					1630:1637	Real-time quantitative polymerase chain reaction	1590:1637	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis	1590:1656	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	10	5	theme	chain	1624:1628	arg1	RT-qPCR					1640:1646	RT-qPCR	1640:1646	RT-qPCR	1640:1646	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	7	6	theme	macrophages	1197:1207	arg1	activation					1183:1192	the activation	1179:1192	the activation of macrophages	1179:1207	Macrophages RAW264.7 cells were employed to evaluate OSP's immunomodulatory activity and the possible mechanism responsible for the activation of macrophages in vitro.					
33892066	10	7	theme	expression	1803:1812	arg1	level					1814:1818	the mRNA expression level	1794:1818	the mRNA expression level of iNOS	1794:1826	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	9	8	theme	RAW264.7 cells	1469:1482	arg1	phagocytosis					1453:1464	The phagocytosis	1449:1464	The phagocytosis of RAW264.7 cells	1449:1482	The phagocytosis of RAW264.7 cells was improved significantly and remarkable changes were observed in the morphology with OSP-treated cells.					
33892066	3	9	from	composition	529:539	arg1	macrophage					510:519	macrophage	510:519	macrophage	510:519	However, the mechanism of polysaccharides on macrophage and the composition of polysaccharides are not known.					
33892066	1	10	theme	RELEVANCE	218:226	arg1	Cordyceps					236:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps	197:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao)	197:263	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	10	theme	RELEVANCE	218:226	arg1	XiaCao					257:262	XiaCao	257:262	XiaCao	257:262	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	10	theme	RELEVANCE	218:226	arg1	medicine					366:373	a traditional Chinese medicine	344:373	a traditional Chinese medicine	344:373	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	10	theme	RELEVANCE	218:226	arg1	complex					278:284	a parasitic complex	266:284	a parasitic complex of a fungus Ophiocordyceps sinensis	266:320	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	10	theme	RELEVANCE	218:226	arg1	caterpillar					328:338	a caterpillar	326:338	a caterpillar	326:338	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	5	11	dep	MATERIALS	809:817	arg1	performed					834:842	performed	834:842	performed a liquid-state fermentation of O. sinensis to produce mycelia	834:904	MATERIALS AND METHODS We performed a liquid-state fermentation of O. sinensis to produce mycelia.					
33892066	12	12	theme	activities	2191:2200	arg1	improvement					2159:2169	an improvement	2156:2169	an improvement of immunomodulation activities	2156:2200	CONCLUSION This study demonstrated that OSP was with a capacity to activate macrophage cells RAW264.7 for an improvement of immunomodulation activities, which was through regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways.					
33892066	2	13	contain	have	419:422	arg1	Polysaccharides					376:390	Polysaccharides	376:390	Polysaccharides extracted from O. sinensis	376:417	Polysaccharides extracted from O. sinensis have immunomodulatory effects on macrophages.					
33892066	2	13	contain	have	419:422	arg2	effects					441:447	immunomodulatory effects	424:447	immunomodulatory effects	424:447	Polysaccharides extracted from O. sinensis have immunomodulatory effects on macrophages.					
33892066	0	14	theme	signaling	177:185	arg1	pathways					187:194	the MAPK and PI3K/Akt signaling pathways	155:194	pathways	187:194	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	4	15	theme	immunomodulatory	740:755	arg1	effect					757:762	the immunomodulatory effect	736:762	the immunomodulatory effect on macrophages	736:777	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	11	16	theme	Western	1883:1889	arg1	analysis					1900:1907	Western blotting analysis	1883:1907	Western blotting analysis	1883:1907	Western blotting analysis showed that the regulation of NO and cytokines was mediated through mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways.					
33892066	11	17	theme	PI3K/Akt	2021:2028	arg1	pathways					2040:2047	PI3K/Akt signaling pathways	2021:2047	PI3K/Akt signaling pathways	2021:2047	Western blotting analysis showed that the regulation of NO and cytokines was mediated through mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways.					
33892066	0	18	from	Ascomycetes	120:130	arg1	RAW264.7 cells					136:149	RAW264.7 cells	136:149	RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways	136:194	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	0	19	theme	PI3K/Akt	168:175	arg1	pathways					187:194	the MAPK and PI3K/Akt signaling pathways	155:194	pathways	187:194	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	12	20	theme	macrophage	2126:2135	arg1	cells					2137:2141	macrophage cells	2126:2141	macrophage cells RAW264.7 for an improvement of immunomodulation activities	2126:2200	CONCLUSION This study demonstrated that OSP was with a capacity to activate macrophage cells RAW264.7 for an improvement of immunomodulation activities, which was through regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways.					
33892066	11	21	theme	mitogen-activated	1977:1993	arg1	MAPK					2011:2014	MAPK	2011:2014	MAPK	2011:2014	Western blotting analysis showed that the regulation of NO and cytokines was mediated through mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways.					
33892066	11	21	theme	mitogen-activated	1977:1993	arg1	kinase					2003:2008	mitogen-activated protein kinase	1977:2008	mitogen-activated protein kinase (MAPK)	1977:2015	Western blotting analysis showed that the regulation of NO and cytokines was mediated through mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways.					
33892066	0	22	theme	liquid	77:82	arg1	culture					84:90	liquid culture	77:90	liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways	77:194	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	4	23	dep	STUDY	582:586	arg1	We					588:589	We	588:589	We	588:589	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	7	24	theme	Macrophages	1051:1061	arg1	RAW264.7 cells					1063:1076	Macrophages RAW264.7 cells	1051:1076	Macrophages RAW264.7 cells	1051:1076	Macrophages RAW264.7 cells were employed to evaluate OSP's immunomodulatory activity and the possible mechanism responsible for the activation of macrophages in vitro.					
33892066	1	25	theme	Chinese	358:364	arg1	Cordyceps					236:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps	197:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao)	197:263	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	25	theme	Chinese	358:364	arg1	medicine					366:373	a traditional Chinese medicine	344:373	a traditional Chinese medicine	344:373	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	9	26	with	morphology	1555:1564	arg1	cells					1583:1587	OSP-treated cells	1571:1587	OSP-treated cells	1571:1587	The phagocytosis of RAW264.7 cells was improved significantly and remarkable changes were observed in the morphology with OSP-treated cells.					
33892066	8	27	theme	2.6	1389:1391	arg1	ratio					1362:1366	the ratio	1358:1366	the ratio of 2.9 : 6.6 : 166 : 2.6	1358:1391	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	0	28	theme	sinensis	110:117	arg1	Ascomycetes					120:130	Ophiocordyceps sinensis (Ascomycetes)	95:131	Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways	95:194	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	12	29	dep	cells	2137:2141	arg1	RAW264.7					2143:2150	RAW264.7	2143:2150	macrophage cells RAW264.7 for an improvement of immunomodulation activities	2126:2200	CONCLUSION This study demonstrated that OSP was with a capacity to activate macrophage cells RAW264.7 for an improvement of immunomodulation activities, which was through regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways.					
33892066	10	30	theme	mRNA	1711:1714	arg1	expression					1716:1725	the mRNA expression	1707:1725	the mRNA expression of pro-inflammatory and anti-inflammatory cytokines	1707:1777	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	4	31	from	composition	612:622	arg1	O.					680:681	O.	680:681	O.	680:681	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	12	32	dep	CONCLUSION	2050:2059	arg1	demonstrated					2072:2083	demonstrated	2072:2083	demonstrated that OSP was with a capacity to activate macrophage cells RAW264.7 for an improvement of immunomodulation activities, which was through regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways	2072:2297	CONCLUSION This study demonstrated that OSP was with a capacity to activate macrophage cells RAW264.7 for an improvement of immunomodulation activities, which was through regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways.					
33892066	8	33	theme	trace	1401:1405	arg1	arabinose					1425:1433	arabinose	1425:1433	arabinose	1425:1433	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	33	theme	trace	1401:1405	arg1	fucose					1417:1422	fucose	1417:1422	fucose	1417:1422	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	33	theme	trace	1401:1405	arg1	amount					1407:1412	a trace amount	1399:1412	a trace amount of fucose, arabinose and rhamnose	1399:1446	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	33	theme	trace	1401:1405	arg1	rhamnose					1439:1446	rhamnose	1439:1446	rhamnose	1439:1446	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	0	34	theme	Immunostimulatory	0:16	arg1	effects					18:24	Immunostimulatory effects	0:24	Immunostimulatory effects of the intracellular polysaccharides	0:61	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	1	35	theme	fungus	291:296	arg1	sinensis					313:320	a fungus Ophiocordyceps sinensis	289:320	a fungus Ophiocordyceps sinensis	289:320	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	4	36	theme	underlying	787:796	arg1	mechanism					798:806	its underlying mechanism	783:806	its underlying mechanism	783:806	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	5	37	theme	O.	875:876	arg1	sinensis					878:885	O. sinensis	875:885	O. sinensis	875:885	MATERIALS AND METHODS We performed a liquid-state fermentation of O. sinensis to produce mycelia.					
33892066	1	38	theme	sinensis	313:320	arg1	Cordyceps					236:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps	197:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao)	197:263	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	38	theme	sinensis	313:320	arg1	complex					278:284	a parasitic complex	266:284	a parasitic complex of a fungus Ophiocordyceps sinensis	266:320	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	38	theme	sinensis	313:320	arg1	caterpillar					328:338	a caterpillar	326:338	a caterpillar	326:338	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	6	39	theme	cellulose	929:937	arg1	column					939:944	The DEAE-Sephadex-A25 cellulose column and Sephadex-G100 gel column chromatography	907:988	column	939:944	The DEAE-Sephadex-A25 cellulose column and Sephadex-G100 gel column chromatography were employed to purify and character the intracellular OSP.					
33892066	12	40	theme	PI3K/Akt	2271:2278	arg1	pathways					2290:2297	PI3K/Akt signaling pathways	2271:2297	PI3K/Akt signaling pathways	2271:2297	CONCLUSION This study demonstrated that OSP was with a capacity to activate macrophage cells RAW264.7 for an improvement of immunomodulation activities, which was through regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways.					
33892066	0	41	theme	intracellular	33:45	arg1	polysaccharides					47:61	the intracellular polysaccharides	29:61	the intracellular polysaccharides	29:61	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	3	42	from	polysaccharides	491:505	arg1	macrophage					510:519	macrophage	510:519	macrophage	510:519	However, the mechanism of polysaccharides on macrophage and the composition of polysaccharides are not known.					
33892066	4	43	dep	O.	680:681	arg1	mycelia					692:698	O. sinensis mycelia	680:698	O. sinensis mycelia (designed as OSP)	680:716	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	4	43	dep	O.	680:681	arg1	designed					701:708	designed	701:708	designed as OSP	701:715	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	12	44	theme	mediators	2248:2256	arg1	regulation					2221:2230	regulation	2221:2230	regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways	2221:2297	CONCLUSION This study demonstrated that OSP was with a capacity to activate macrophage cells RAW264.7 for an improvement of immunomodulation activities, which was through regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways.					
33892066	3	45	from	macrophage	510:519	arg1	composition					529:539	the composition	525:539	the composition of polysaccharides	525:558	However, the mechanism of polysaccharides on macrophage and the composition of polysaccharides are not known.					
33892066	3	45	from	macrophage	510:519	arg1	mechanism					478:486	the mechanism	474:486	the mechanism of polysaccharides on macrophage	474:519	However, the mechanism of polysaccharides on macrophage and the composition of polysaccharides are not known.					
33892066	10	46	theme	cytokines	1769:1777	arg1	expression					1716:1725	the mRNA expression	1707:1725	the mRNA expression of pro-inflammatory and anti-inflammatory cytokines	1707:1777	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	4	47	from	effect	757:762	arg1	macrophages					767:777	macrophages	767:777	macrophages	767:777	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	10	48	contain	had	1680:1682	arg2	ability					1687:1693	an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells	1684:1880	an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells	1684:1880	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	10	48	contain	had	1680:1682	arg1	OSP					1676:1678	OSP	1676:1678	OSP	1676:1678	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	10	49	theme	polymerase	1613:1622	arg1	reaction					1630:1637	Real-time quantitative polymerase chain reaction	1590:1637	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis	1590:1656	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	10	49	theme	polymerase	1613:1622	arg1	RT-qPCR					1640:1646	RT-qPCR	1640:1646	RT-qPCR	1640:1646	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	3	50	theme	polysaccharides	491:505	arg1	composition					529:539	the composition	525:539	the composition of polysaccharides	525:558	However, the mechanism of polysaccharides on macrophage and the composition of polysaccharides are not known.					
33892066	3	50	theme	polysaccharides	491:505	arg1	mechanism					478:486	the mechanism	474:486	the mechanism of polysaccharides on macrophage	474:519	However, the mechanism of polysaccharides on macrophage and the composition of polysaccharides are not known.					
33892066	10	51	theme	mRNA	1798:1801	arg1	level					1814:1818	the mRNA expression level	1794:1818	the mRNA expression level of iNOS	1794:1826	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	4	52	theme	polysaccharides	659:673	arg1	structure					628:636	structure	628:636	structure	628:636	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	4	52	theme	polysaccharides	659:673	arg1	composition					612:622	composition	612:622	composition	612:622	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	10	53	theme	Real-time	1590:1598	arg1	reaction					1630:1637	Real-time quantitative polymerase chain reaction	1590:1637	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis	1590:1656	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	10	53	theme	Real-time	1590:1598	arg1	RT-qPCR					1640:1646	RT-qPCR	1640:1646	RT-qPCR	1640:1646	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	3	54	from	mechanism	478:486	arg1	macrophage					510:519	macrophage	510:519	macrophage	510:519	However, the mechanism of polysaccharides on macrophage and the composition of polysaccharides are not known.					
33892066	3	55	theme	polysaccharides	544:558	arg1	composition					529:539	the composition	525:539	the composition of polysaccharides	525:558	However, the mechanism of polysaccharides on macrophage and the composition of polysaccharides are not known.					
33892066	3	55	theme	polysaccharides	544:558	arg1	mechanism					478:486	the mechanism	474:486	the mechanism of polysaccharides on macrophage	474:519	However, the mechanism of polysaccharides on macrophage and the composition of polysaccharides are not known.					
33892066	1	56	theme	parasitic	268:276	arg1	complex					278:284	a parasitic complex	266:284	a parasitic complex of a fungus Ophiocordyceps sinensis	266:320	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	56	theme	parasitic	268:276	arg1	Cordyceps					236:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps	197:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao)	197:263	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	8	57	theme	molecular	1239:1247	arg1	weight					1249:1254	The average molecular weight	1227:1254	RESULTS The average molecular weight of OSP	1219:1261	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	10	58	dep	ability	1687:1693	arg1	induce					1787:1792	induce	1787:1792	to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells	1784:1880	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	10	58	dep	ability	1687:1693	arg1	regulate					1698:1705	regulate	1698:1705	to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines	1695:1777	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	10	59	theme	iNOS	1823:1826	arg1	level					1814:1818	the mRNA expression level	1794:1818	the mRNA expression level of iNOS	1794:1826	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	6	60	theme	gel	964:966	arg1	chromatography					975:988	The DEAE-Sephadex-A25 cellulose column and Sephadex-G100 gel column chromatography	907:988	chromatography	975:988	The DEAE-Sephadex-A25 cellulose column and Sephadex-G100 gel column chromatography were employed to purify and character the intracellular OSP.					
33892066	10	61	theme	reaction	1630:1637	arg1	analysis					1649:1656	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis	1590:1656	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis	1590:1656	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	8	62	with	galactose	1343:1351	arg1	ratio					1362:1366	the ratio	1358:1366	the ratio of 2.9 : 6.6 : 166 : 2.6	1358:1391	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	63	dep	RESULTS	1219:1225	arg1	weight					1249:1254	The average molecular weight	1227:1254	RESULTS The average molecular weight of OSP	1219:1261	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	10	64	theme	dependent	1847:1855	arg1	manner					1857:1862	a concentration dependent manner	1831:1862	a concentration dependent manner	1831:1862	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	1	65	theme	ETHNOPHARMACOLOGICAL	197:216	arg1	Cordyceps					236:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps	197:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao)	197:263	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	65	theme	ETHNOPHARMACOLOGICAL	197:216	arg1	XiaCao					257:262	XiaCao	257:262	XiaCao	257:262	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	65	theme	ETHNOPHARMACOLOGICAL	197:216	arg1	medicine					366:373	a traditional Chinese medicine	344:373	a traditional Chinese medicine	344:373	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	65	theme	ETHNOPHARMACOLOGICAL	197:216	arg1	complex					278:284	a parasitic complex	266:284	a parasitic complex of a fungus Ophiocordyceps sinensis	266:320	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	65	theme	ETHNOPHARMACOLOGICAL	197:216	arg1	caterpillar					328:338	a caterpillar	326:338	a caterpillar	326:338	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	7	66	theme	responsible	1163:1173	arg1	mechanism					1153:1161	the possible mechanism	1140:1161	the possible mechanism responsible for the activation of macrophages	1140:1207	Macrophages RAW264.7 cells were employed to evaluate OSP's immunomodulatory activity and the possible mechanism responsible for the activation of macrophages in vitro.					
33892066	12	67	theme	signaling	2280:2288	arg1	pathways					2290:2297	PI3K/Akt signaling pathways	2271:2297	PI3K/Akt signaling pathways	2271:2297	CONCLUSION This study demonstrated that OSP was with a capacity to activate macrophage cells RAW264.7 for an improvement of immunomodulation activities, which was through regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways.					
33892066	1	68	theme	Chinese	228:234	arg1	Cordyceps					236:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps	197:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao)	197:263	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	68	theme	Chinese	228:234	arg1	XiaCao					257:262	XiaCao	257:262	XiaCao	257:262	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	68	theme	Chinese	228:234	arg1	medicine					366:373	a traditional Chinese medicine	344:373	a traditional Chinese medicine	344:373	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	68	theme	Chinese	228:234	arg1	complex					278:284	a parasitic complex	266:284	a parasitic complex of a fungus Ophiocordyceps sinensis	266:320	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	68	theme	Chinese	228:234	arg1	caterpillar					328:338	a caterpillar	326:338	a caterpillar	326:338	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	6	69	theme	intracellular	1032:1044	arg1	OSP					1046:1048	the intracellular OSP	1028:1048	the intracellular OSP	1028:1048	The DEAE-Sephadex-A25 cellulose column and Sephadex-G100 gel column chromatography were employed to purify and character the intracellular OSP.					
33892066	12	70	theme	immunomodulation	2174:2189	arg1	activities					2191:2200	immunomodulation activities	2174:2200	immunomodulation activities	2174:2200	CONCLUSION This study demonstrated that OSP was with a capacity to activate macrophage cells RAW264.7 for an improvement of immunomodulation activities, which was through regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways.					
33892066	7	71	theme	possible	1144:1151	arg1	mechanism					1153:1161	the possible mechanism	1140:1161	the possible mechanism responsible for the activation of macrophages	1140:1207	Macrophages RAW264.7 cells were employed to evaluate OSP's immunomodulatory activity and the possible mechanism responsible for the activation of macrophages in vitro.					
33892066	11	72	theme	signaling	2030:2038	arg1	pathways					2040:2047	PI3K/Akt signaling pathways	2021:2047	PI3K/Akt signaling pathways	2021:2047	Western blotting analysis showed that the regulation of NO and cytokines was mediated through mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways.					
33892066	4	73	from	O.	680:681	arg1	structure					628:636	structure	628:636	structure	628:636	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	4	73	from	O.	680:681	arg1	composition					612:622	composition	612:622	composition	612:622	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	4	73	from	O.	680:681	arg1	polysaccharides					659:673	the intracellular polysaccharides	641:673	the intracellular polysaccharides from O. sinensis mycelia (designed as OSP)	641:716	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	9	74	theme	remarkable	1515:1524	arg1	changes					1526:1532	remarkable changes	1515:1532	remarkable changes	1515:1532	The phagocytosis of RAW264.7 cells was improved significantly and remarkable changes were observed in the morphology with OSP-treated cells.					
33892066	0	75	from	culture	84:90	arg1	RAW264.7 cells					136:149	RAW264.7 cells	136:149	RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways	136:194	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	9	76	located	observed	1539:1546	arg2	changes					1526:1532	remarkable changes	1515:1532	remarkable changes	1515:1532	The phagocytosis of RAW264.7 cells was improved significantly and remarkable changes were observed in the morphology with OSP-treated cells.					
33892066	9	76	located	observed	1539:1546	arg1	morphology					1555:1564	the morphology	1551:1564	the morphology with OSP-treated cells	1551:1587	The phagocytosis of RAW264.7 cells was improved significantly and remarkable changes were observed in the morphology with OSP-treated cells.					
33892066	11	77	theme	blotting	1891:1898	arg1	analysis					1900:1907	Western blotting analysis	1883:1907	Western blotting analysis	1883:1907	Western blotting analysis showed that the regulation of NO and cytokines was mediated through mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways.					
33892066	7	78	theme	immunomodulatory	1110:1125	arg1	activity					1127:1134	OSP's immunomodulatory activity	1104:1134	OSP's immunomodulatory activity	1104:1134	Macrophages RAW264.7 cells were employed to evaluate OSP's immunomodulatory activity and the possible mechanism responsible for the activation of macrophages in vitro.					
33892066	12	79	theme	inflammatory	2235:2246	arg1	mediators					2248:2256	inflammatory mediators	2235:2256	inflammatory mediators	2235:2256	CONCLUSION This study demonstrated that OSP was with a capacity to activate macrophage cells RAW264.7 for an improvement of immunomodulation activities, which was through regulation of inflammatory mediators via MAPK and PI3K/Akt signaling pathways.					
33892066	8	80	with	xylose	1313:1318	arg1	arabinose					1425:1433	arabinose	1425:1433	arabinose	1425:1433	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	80	with	xylose	1313:1318	arg1	fucose					1417:1422	fucose	1417:1422	fucose	1417:1422	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	80	with	xylose	1313:1318	arg1	amount					1407:1412	a trace amount	1399:1412	a trace amount of fucose, arabinose and rhamnose	1399:1446	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	80	with	xylose	1313:1318	arg1	rhamnose					1439:1446	rhamnose	1439:1446	rhamnose	1439:1446	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	5	81	theme	sinensis	878:885	arg1	fermentation					859:870	a liquid-state fermentation	844:870	a liquid-state fermentation of O. sinensis	844:885	MATERIALS AND METHODS We performed a liquid-state fermentation of O. sinensis to produce mycelia.					
33892066	4	82	from	structure	628:636	arg1	O.					680:681	O.	680:681	O.	680:681	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	0	83	theme	Ophiocordyceps	95:108	arg1	Ascomycetes					120:130	Ophiocordyceps sinensis (Ascomycetes)	95:131	Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways	95:194	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	1	84	theme	traditional	346:356	arg1	Cordyceps					236:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps	197:244	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao)	197:263	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	1	84	theme	traditional	346:356	arg1	medicine					366:373	a traditional Chinese medicine	344:373	a traditional Chinese medicine	344:373	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	9	85	theme	OSP-treated	1571:1581	arg1	cells					1583:1587	OSP-treated cells	1571:1587	OSP-treated cells	1571:1587	The phagocytosis of RAW264.7 cells was improved significantly and remarkable changes were observed in the morphology with OSP-treated cells.					
33892066	2	86	dep	O.	407:408	arg1	sinensis					410:417	O. sinensis	407:417	O. sinensis	407:417	Polysaccharides extracted from O. sinensis have immunomodulatory effects on macrophages.					
33892066	4	87	from	mechanism	798:806	arg1	macrophages					767:777	macrophages	767:777	macrophages	767:777	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	11	88	theme	protein	1995:2001	arg1	MAPK					2011:2014	MAPK	2011:2014	MAPK	2011:2014	Western blotting analysis showed that the regulation of NO and cytokines was mediated through mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways.					
33892066	11	88	theme	protein	1995:2001	arg1	kinase					2003:2008	mitogen-activated protein kinase	1977:2008	mitogen-activated protein kinase (MAPK)	1977:2015	Western blotting analysis showed that the regulation of NO and cytokines was mediated through mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways.					
33892066	5	89	theme	liquid-state	846:857	arg1	fermentation					859:870	a liquid-state fermentation	844:870	a liquid-state fermentation of O. sinensis	844:885	MATERIALS AND METHODS We performed a liquid-state fermentation of O. sinensis to produce mycelia.					
33892066	11	90	theme	cytokines	1946:1954	arg1	regulation					1925:1934	the regulation	1921:1934	the regulation of NO and cytokines	1921:1954	Western blotting analysis showed that the regulation of NO and cytokines was mediated through mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling pathways.					
33892066	1	91	theme	Ophiocordyceps	298:311	arg1	sinensis					313:320	a fungus Ophiocordyceps sinensis	289:320	a fungus Ophiocordyceps sinensis	289:320	ETHNOPHARMACOLOGICAL RELEVANCE Chinese Cordyceps (DongChong XiaCao), a parasitic complex of a fungus Ophiocordyceps sinensis and a caterpillar, is a traditional Chinese medicine.					
33892066	10	92	theme	pro-inflammatory	1730:1745	arg1	cytokines					1769:1777	pro-inflammatory and anti-inflammatory cytokines	1730:1777	cytokines	1769:1777	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	0	93	theme	polysaccharides	47:61	arg1	effects					18:24	Immunostimulatory effects	0:24	Immunostimulatory effects of the intracellular polysaccharides	0:61	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	2	94	theme	immunomodulatory	424:439	arg1	effects					441:447	immunomodulatory effects	424:447	immunomodulatory effects	424:447	Polysaccharides extracted from O. sinensis have immunomodulatory effects on macrophages.					
33892066	8	95	theme	fucose	1417:1422	arg1	arabinose					1425:1433	arabinose	1425:1433	arabinose	1425:1433	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	95	theme	fucose	1417:1422	arg1	fucose					1417:1422	fucose	1417:1422	fucose	1417:1422	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	95	theme	fucose	1417:1422	arg1	amount					1407:1412	a trace amount	1399:1412	a trace amount of fucose, arabinose and rhamnose	1399:1446	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	95	theme	fucose	1417:1422	arg1	rhamnose					1439:1446	rhamnose	1439:1446	rhamnose	1439:1446	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	0	96	theme	Ascomycetes	120:130	arg1	culture					84:90	liquid culture	77:90	liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways	77:194	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	4	97	theme	STUDY	582:586	arg1	AIM					575:577	AIM	575:577	AIM OF STUDY We	575:589	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	8	98	theme	arabinose	1425:1433	arg1	arabinose					1425:1433	arabinose	1425:1433	arabinose	1425:1433	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	98	theme	arabinose	1425:1433	arg1	fucose					1417:1422	fucose	1417:1422	fucose	1417:1422	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	98	theme	arabinose	1425:1433	arg1	amount					1407:1412	a trace amount	1399:1412	a trace amount of fucose, arabinose and rhamnose	1399:1446	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	98	theme	arabinose	1425:1433	arg1	rhamnose					1439:1446	rhamnose	1439:1446	rhamnose	1439:1446	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	4	99	theme	intracellular	645:657	arg1	polysaccharides					659:673	the intracellular polysaccharides	641:673	the intracellular polysaccharides from O. sinensis mycelia (designed as OSP)	641:716	AIM OF STUDY We aimed to investigate composition and structure of the intracellular polysaccharides from O. sinensis mycelia (designed as OSP), and evaluate its the immunomodulatory effect on macrophages and its underlying mechanism.					
33892066	8	100	theme	rhamnose	1439:1446	arg1	arabinose					1425:1433	arabinose	1425:1433	arabinose	1425:1433	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	100	theme	rhamnose	1439:1446	arg1	fucose					1417:1422	fucose	1417:1422	fucose	1417:1422	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	100	theme	rhamnose	1439:1446	arg1	amount					1407:1412	a trace amount	1399:1412	a trace amount of fucose, arabinose and rhamnose	1399:1446	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	8	100	theme	rhamnose	1439:1446	arg1	rhamnose					1439:1446	rhamnose	1439:1446	rhamnose	1439:1446	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	10	101	theme	anti-inflammatory	1751:1767	arg1	cytokines					1769:1777	pro-inflammatory and anti-inflammatory cytokines	1730:1777	cytokines	1769:1777	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	8	102	theme	average	1231:1237	arg1	weight					1249:1254	The average molecular weight	1227:1254	RESULTS The average molecular weight of OSP	1219:1261	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
33892066	0	103	from	RAW264.7 cells	136:149	arg1	culture					84:90	liquid culture	77:90	liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways	77:194	Immunostimulatory effects of the intracellular polysaccharides isolated from liquid culture of Ophiocordyceps sinensis (Ascomycetes) on RAW264.7 cells via the MAPK and PI3K/Akt signaling pathways.					
33892066	10	104	theme	quantitative	1600:1611	arg1	reaction					1630:1637	Real-time quantitative polymerase chain reaction	1590:1637	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis	1590:1656	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	10	104	theme	quantitative	1600:1611	arg1	RT-qPCR					1640:1646	RT-qPCR	1640:1646	RT-qPCR	1640:1646	Real-time quantitative polymerase chain reaction (RT-qPCR) analysis demonstrated that OSP had an ability to regulate the mRNA expression of pro-inflammatory and anti-inflammatory cytokines, and to induce the mRNA expression level of iNOS in a concentration dependent manner in RAW264.7 cells.					
33892066	8	105	with	glucose	1330:1336	arg1	ratio					1362:1366	the ratio	1358:1366	the ratio of 2.9 : 6.6 : 166 : 2.6	1358:1391	RESULTS The average molecular weight of OSP was distributed at 27,972 Da, OSP was composed of xylose, mannose, glucose, and galactose with the ratio of 2.9 : 6.6 : 166 : 2.6, with a trace amount of fucose, arabinose and rhamnose.					
32946934	0	0	theme	oxidative	91:99	arg1	stress					101:106	cellular oxidative stress	82:106	cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus	82:164	Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus.					
32946934	5	1	theme	compositive	909:919	arg1	mechanism					939:947	a compositive oxidation defense mechanism	907:947	a compositive oxidation defense mechanism than CP	907:955	P-CP provided a more significant protective effect against hydrogen peroxide-induced oxidative stress in RAW264.7 cells by a compositive oxidation defense mechanism than CP.					
32946934	6	2	theme	antioxidant	1036:1046	arg1	SOD					1077:1079	SOD	1077:1079	SOD	1077:1079	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	6	2	theme	antioxidant	1036:1046	arg1	dismutase					1066:1074	antioxidant enzyme superoxide dismutase	1036:1074	antioxidant enzyme superoxide dismutase (SOD)	1036:1080	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	0	3	dep	Cyclocarya	146:155	arg1	paliurus					157:164	Cyclocarya paliurus	146:164	Cyclocarya paliurus	146:164	Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus.					
32946934	4	4	theme	excellent	690:698	arg1	capacities					711:720	excellent scavenging capacities	690:720	excellent scavenging capacities	690:720	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP) showed excellent scavenging capacities against DPPH and hydroxyl radical in a dose-dependent manner.					
32946934	0	5	theme	cellular	82:89	arg1	stress					101:106	cellular oxidative stress	82:106	cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus	82:164	Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus.					
32946934	2	6	theme	phosphorylated	411:424	arg1	derivatives					426:436	phosphorylated derivatives	411:436	phosphorylated derivatives	411:436	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	1	7	theme	sodium	275:280	arg1	tripolyphosphate					282:297	sodium tripolyphosphate	275:297	sodium tripolyphosphate	275:297	In this study, a phosphorylated derivative of Cyclocarya paliurus polysaccharide (CP) was synthesized using sodium tripolyphosphate and sodium trimetaphosphate.					
32946934	5	8	theme	oxidative	869:877	arg1	stress					879:884	hydrogen peroxide-induced oxidative stress	843:884	hydrogen peroxide-induced oxidative stress in RAW264.7 cells	843:902	P-CP provided a more significant protective effect against hydrogen peroxide-induced oxidative stress in RAW264.7 cells by a compositive oxidation defense mechanism than CP.					
32946934	3	9	from	structure	609:617	arg1	composition					580:590	chemical composition	571:590	chemical composition	571:590	Results showed that phosphorylated modification caused a significant change in chemical composition and the apparent structure of CP.					
32946934	2	10	theme	Antioxidant	328:338	arg1	activities					340:349	Antioxidant activities	328:349	Antioxidant activities	328:349	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	6	11	theme	dismutase	1066:1074	arg1	contents					1024:1031	the contents	1020:1031	the contents of antioxidant enzyme superoxide dismutase (SOD)	1020:1080	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	6	12	theme	damaged	1140:1146	arg1	cells					1148:1152	oxidative damaged cells	1130:1152	oxidative damaged cells	1130:1152	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	6	13	theme	malondialdehyde	1097:1111	arg1	content					1119:1125	malondialdehyde (MDA) content	1097:1125	malondialdehyde (MDA) content in oxidative damaged cells	1097:1152	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	2	14	theme	derivatives	426:436	arg1	activities					340:349	Antioxidant activities	328:349	Antioxidant activities	328:349	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	2	14	theme	derivatives	426:436	arg1	effects					370:376	cytoprotective effects	355:376	cytoprotective effects	355:376	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	4	15	theme	scavenging	700:709	arg1	capacities					711:720	excellent scavenging capacities	690:720	excellent scavenging capacities	690:720	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP) showed excellent scavenging capacities against DPPH and hydroxyl radical in a dose-dependent manner.					
32946934	0	16	theme	polysaccharide	126:139	arg1	stress					101:106	cellular oxidative stress	82:106	cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus	82:164	Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus.					
32946934	6	17	theme	superoxide	1055:1064	arg1	SOD					1077:1079	SOD	1077:1079	SOD	1077:1079	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	6	17	theme	superoxide	1055:1064	arg1	dismutase					1066:1074	antioxidant enzyme superoxide dismutase	1036:1074	antioxidant enzyme superoxide dismutase (SOD)	1036:1080	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	0	18	theme	phosphorylated	111:124	arg1	polysaccharide					126:139	phosphorylated polysaccharide	111:139	phosphorylated polysaccharide from Cyclocarya paliurus	111:164	Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus.					
32946934	6	19	theme	enzyme	1048:1053	arg1	SOD					1077:1079	SOD	1077:1079	SOD	1077:1079	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	6	19	theme	enzyme	1048:1053	arg1	dismutase					1066:1074	antioxidant enzyme superoxide dismutase	1036:1074	antioxidant enzyme superoxide dismutase (SOD)	1036:1080	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	5	20	theme	defense	931:937	arg1	mechanism					939:947	a compositive oxidation defense mechanism	907:947	a compositive oxidation defense mechanism than CP	907:955	P-CP provided a more significant protective effect against hydrogen peroxide-induced oxidative stress in RAW264.7 cells by a compositive oxidation defense mechanism than CP.					
32946934	6	21	theme	scavenging	1170:1179	arg1	ROS					1181:1183	scavenging ROS	1170:1183	scavenging ROS	1170:1183	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	1	22	theme	phosphorylated	184:197	arg1	derivative					199:208	a phosphorylated derivative	182:208	a phosphorylated derivative of Cyclocarya paliurus polysaccharide (CP)	182:251	In this study, a phosphorylated derivative of Cyclocarya paliurus polysaccharide (CP) was synthesized using sodium tripolyphosphate and sodium trimetaphosphate.					
32946934	5	23	theme	oxidation	921:929	arg1	mechanism					939:947	a compositive oxidation defense mechanism	907:947	a compositive oxidation defense mechanism than CP	907:955	P-CP provided a more significant protective effect against hydrogen peroxide-induced oxidative stress in RAW264.7 cells by a compositive oxidation defense mechanism than CP.					
32946934	6	24	theme	oxidative	1130:1138	arg1	cells					1148:1152	oxidative damaged cells	1130:1152	oxidative damaged cells	1130:1152	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	6	25	theme	antioxidant	1333:1343	arg1	activity					1345:1352	the intracellular antioxidant activity	1315:1352	the intracellular antioxidant activity	1315:1352	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	2	26	theme	various	466:472	arg1	systems					483:489	various in vitro systems	466:489	various in vitro systems	466:489	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	1	27	theme	sodium	303:308	arg1	trimetaphosphate					310:325	sodium trimetaphosphate	303:325	sodium trimetaphosphate	303:325	In this study, a phosphorylated derivative of Cyclocarya paliurus polysaccharide (CP) was synthesized using sodium tripolyphosphate and sodium trimetaphosphate.					
32946934	6	28	theme	oxidative	976:984	arg1	stress					986:991	oxidative stress	976:991	oxidative stress	976:991	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	2	29	theme	cytoprotective	355:368	arg1	effects					370:376	cytoprotective effects	355:376	cytoprotective effects	355:376	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	3	30	theme	phosphorylated	512:525	arg1	modification					527:538	phosphorylated modification	512:538	phosphorylated modification	512:538	Results showed that phosphorylated modification caused a significant change in chemical composition and the apparent structure of CP.					
32946934	4	31	theme	polysaccharide	661:674	arg1	P-CP					677:680	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP)	626:681	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP)	626:681	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP) showed excellent scavenging capacities against DPPH and hydroxyl radical in a dose-dependent manner.					
32946934	6	32	from	content	1119:1125	arg1	cells					1148:1152	oxidative damaged cells	1130:1152	oxidative damaged cells	1130:1152	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	7	33	theme	CP	1528:1529	arg1	development					1497:1507	development	1497:1507	development	1497:1507	This study suggested that phosphorylated modification is an effective method to improve the antioxidant activities of CP, so as to expand the development and application of CP.					
32946934	7	33	theme	CP	1528:1529	arg1	application					1513:1523	application	1513:1523	application	1513:1523	This study suggested that phosphorylated modification is an effective method to improve the antioxidant activities of CP, so as to expand the development and application of CP.					
32946934	1	34	theme	Cyclocarya	213:222	arg1	CP					249:250	CP	249:250	CP	249:250	In this study, a phosphorylated derivative of Cyclocarya paliurus polysaccharide (CP) was synthesized using sodium tripolyphosphate and sodium trimetaphosphate.					
32946934	1	34	theme	Cyclocarya	213:222	arg1	polysaccharide					233:246	Cyclocarya paliurus polysaccharide	213:246	Cyclocarya paliurus polysaccharide (CP)	213:251	In this study, a phosphorylated derivative of Cyclocarya paliurus polysaccharide (CP) was synthesized using sodium tripolyphosphate and sodium trimetaphosphate.					
32946934	6	35	theme	intracellular	1319:1331	arg1	activity					1345:1352	the intracellular antioxidant activity	1315:1352	the intracellular antioxidant activity	1315:1352	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	6	36	theme	apoptosis	1243:1251	arg1	capacity					1253:1260	apoptosis capacity	1243:1260	apoptosis capacity	1243:1260	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	1	37	theme	paliurus	224:231	arg1	CP					249:250	CP	249:250	CP	249:250	In this study, a phosphorylated derivative of Cyclocarya paliurus polysaccharide (CP) was synthesized using sodium tripolyphosphate and sodium trimetaphosphate.					
32946934	1	37	theme	paliurus	224:231	arg1	polysaccharide					233:246	Cyclocarya paliurus polysaccharide	213:246	Cyclocarya paliurus polysaccharide (CP)	213:251	In this study, a phosphorylated derivative of Cyclocarya paliurus polysaccharide (CP) was synthesized using sodium tripolyphosphate and sodium trimetaphosphate.					
32946934	3	38	theme	apparent	600:607	arg1	structure					609:617	the apparent structure	596:617	the apparent structure of CP	596:623	Results showed that phosphorylated modification caused a significant change in chemical composition and the apparent structure of CP.					
32946934	4	39	theme	dose-dependent	761:774	arg1	manner					776:781	a dose-dependent manner	759:781	a dose-dependent manner	759:781	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP) showed excellent scavenging capacities against DPPH and hydroxyl radical in a dose-dependent manner.					
32946934	6	40	theme	cycle	1214:1218	arg1	distribution					1220:1231	cell cycle distribution	1209:1231	cell cycle distribution	1209:1231	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	1	41	theme	polysaccharide	233:246	arg1	derivative					199:208	a phosphorylated derivative	182:208	a phosphorylated derivative of Cyclocarya paliurus polysaccharide (CP)	182:251	In this study, a phosphorylated derivative of Cyclocarya paliurus polysaccharide (CP) was synthesized using sodium tripolyphosphate and sodium trimetaphosphate.					
32946934	0	42	theme	antioxidant	31:41	arg1	activity					43:50	antioxidant activity	31:50	antioxidant activity	31:50	Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus.					
32946934	4	43	theme	Phosphorylated	626:639	arg1	P-CP					677:680	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP)	626:681	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP)	626:681	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP) showed excellent scavenging capacities against DPPH and hydroxyl radical in a dose-dependent manner.					
32946934	5	44	theme	peroxide-induced	852:867	arg1	stress					879:884	hydrogen peroxide-induced oxidative stress	843:884	hydrogen peroxide-induced oxidative stress in RAW264.7 cells	843:902	P-CP provided a more significant protective effect against hydrogen peroxide-induced oxidative stress in RAW264.7 cells by a compositive oxidation defense mechanism than CP.					
32946934	4	45	theme	hydroxyl	739:746	arg1	radical					748:754	hydroxyl radical	739:754	hydroxyl radical	739:754	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP) showed excellent scavenging capacities against DPPH and hydroxyl radical in a dose-dependent manner.					
32946934	3	46	theme	significant	549:559	arg1	change					561:566	a significant change	547:566	a significant change in chemical composition	547:590	Results showed that phosphorylated modification caused a significant change in chemical composition and the apparent structure of CP.					
32946934	0	47	theme	protective	56:65	arg1	effect					67:72	protective effect	56:72	protective effect	56:72	Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus.					
32946934	7	48	theme	phosphorylated	1381:1394	arg1	modification					1396:1407	phosphorylated modification	1381:1407	phosphorylated modification	1381:1407	This study suggested that phosphorylated modification is an effective method to improve the antioxidant activities of CP, so as to expand the development and application of CP.					
32946934	7	48	theme	phosphorylated	1381:1394	arg1	method					1425:1430	an effective method	1412:1430	an effective method	1412:1430	This study suggested that phosphorylated modification is an effective method to improve the antioxidant activities of CP, so as to expand the development and application of CP.					
32946934	5	49	from	stress	879:884	arg1	RAW264.7 cells					889:902	RAW264.7 cells	889:902	RAW264.7 cells	889:902	P-CP provided a more significant protective effect against hydrogen peroxide-induced oxidative stress in RAW264.7 cells by a compositive oxidation defense mechanism than CP.					
32946934	7	50	theme	CP	1473:1474	arg1	activities					1459:1468	the antioxidant activities	1443:1468	the antioxidant activities of CP	1443:1474	This study suggested that phosphorylated modification is an effective method to improve the antioxidant activities of CP, so as to expand the development and application of CP.					
32946934	7	51	dep	method	1425:1430	arg1	improve					1435:1441	improve	1435:1441	to improve the antioxidant activities of CP	1432:1474	This study suggested that phosphorylated modification is an effective method to improve the antioxidant activities of CP, so as to expand the development and application of CP.					
32946934	4	52	theme	paliurus	652:659	arg1	P-CP					677:680	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP)	626:681	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP)	626:681	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP) showed excellent scavenging capacities against DPPH and hydroxyl radical in a dose-dependent manner.					
32946934	3	53	from	change	561:566	arg1	composition					580:590	chemical composition	571:590	chemical composition	571:590	Results showed that phosphorylated modification caused a significant change in chemical composition and the apparent structure of CP.					
32946934	2	54	theme	polysaccharide	392:405	arg1	activities					340:349	Antioxidant activities	328:349	Antioxidant activities	328:349	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	2	54	theme	polysaccharide	392:405	arg1	effects					370:376	cytoprotective effects	355:376	cytoprotective effects	355:376	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	4	55	theme	Cyclocarya	641:650	arg1	P-CP					677:680	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP)	626:681	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP)	626:681	Phosphorylated Cyclocarya paliurus polysaccharide (P-CP) showed excellent scavenging capacities against DPPH and hydroxyl radical in a dose-dependent manner.					
32946934	5	56	theme	significant	805:815	arg1	effect					828:833	a more significant protective effect	798:833	a more significant protective effect against hydrogen peroxide-induced oxidative stress in RAW264.7 cells	798:902	P-CP provided a more significant protective effect against hydrogen peroxide-induced oxidative stress in RAW264.7 cells by a compositive oxidation defense mechanism than CP.					
32946934	7	57	theme	antioxidant	1447:1457	arg1	activities					1459:1468	the antioxidant activities	1443:1468	the antioxidant activities of CP	1443:1474	This study suggested that phosphorylated modification is an effective method to improve the antioxidant activities of CP, so as to expand the development and application of CP.					
32946934	2	58	theme	unmodified	381:390	arg1	polysaccharide					392:405	unmodified polysaccharide	381:405	unmodified polysaccharide	381:405	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	5	59	theme	protective	817:826	arg1	effect					828:833	a more significant protective effect	798:833	a more significant protective effect against hydrogen peroxide-induced oxidative stress in RAW264.7 cells	798:902	P-CP provided a more significant protective effect against hydrogen peroxide-induced oxidative stress in RAW264.7 cells by a compositive oxidation defense mechanism than CP.					
32946934	6	60	theme	cell	1276:1279	arg1	viability					1281:1289	cell viability	1276:1289	cell viability	1276:1289	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	3	61	theme	chemical	571:578	arg1	composition					580:590	chemical composition	571:590	chemical composition	571:590	Results showed that phosphorylated modification caused a significant change in chemical composition and the apparent structure of CP.					
32946934	7	62	theme	effective	1415:1423	arg1	modification					1396:1407	phosphorylated modification	1381:1407	phosphorylated modification	1381:1407	This study suggested that phosphorylated modification is an effective method to improve the antioxidant activities of CP, so as to expand the development and application of CP.					
32946934	7	62	theme	effective	1415:1423	arg1	method					1425:1430	an effective method	1412:1430	an effective method	1412:1430	This study suggested that phosphorylated modification is an effective method to improve the antioxidant activities of CP, so as to expand the development and application of CP.					
32946934	0	63	from	stress	101:106	arg1	Cyclocarya					146:155	Cyclocarya	146:155	Cyclocarya	146:155	Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus.					
32946934	6	64	theme	cell	1209:1212	arg1	distribution					1220:1231	cell cycle distribution	1209:1231	cell cycle distribution	1209:1231	P-CP could reduce oxidative stress by significantly enhancing the contents of antioxidant enzyme superoxide dismutase (SOD) and decreasing malondialdehyde (MDA) content in oxidative damaged cells, in addition to scavenging ROS directly and regulating cell cycle distribution, reducing apoptosis capacity and improving cell viability, consequently achieving the intracellular antioxidant activity.					
32946934	2	65	theme	in	474:475	arg1	systems					483:489	various in vitro systems	466:489	various in vitro systems	466:489	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	0	66	from	Cyclocarya	146:155	arg1	stress					101:106	cellular oxidative stress	82:106	cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus	82:164	Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus.					
32946934	0	66	from	Cyclocarya	146:155	arg1	polysaccharide					126:139	phosphorylated polysaccharide	111:139	phosphorylated polysaccharide from Cyclocarya paliurus	111:164	Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus.					
32946934	7	67	dep	development	1497:1507	arg1	the					1493:1495	the	1493:1495	the	1493:1495	This study suggested that phosphorylated modification is an effective method to improve the antioxidant activities of CP, so as to expand the development and application of CP.					
32946934	2	68	dep	in	474:475	arg1	vitro					477:481	vitro	477:481	vitro	477:481	Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems.					
32946934	3	69	theme	CP	622:623	arg1	structure					609:617	the apparent structure	596:617	the apparent structure of CP	596:623	Results showed that phosphorylated modification caused a significant change in chemical composition and the apparent structure of CP.					
32946934	3	69	theme	CP	622:623	arg1	change					561:566	a significant change	547:566	a significant change in chemical composition	547:590	Results showed that phosphorylated modification caused a significant change in chemical composition and the apparent structure of CP.					
33483072	0	0	theme	modified	60:67	arg1	chitin					69:74	modified chitin	60:74	modified chitin	60:74	A new type of bilayer dural substitute candidate made up of modified chitin and bacterial cellulose.					
33483072	4	1	theme	dense	499:503	arg1	film					538:541	a composite polysaccharid film	512:541	a composite polysaccharid film	512:541	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	4	1	theme	dense	499:503	arg1	layer					505:509	The dense layer	495:509	The dense layer	495:509	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	5	2	theme	polysaccharid	656:668	arg1	layer					637:641	The porous layer	626:641	The porous layer	626:641	The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC.					
33483072	5	2	theme	polysaccharid	656:668	arg1	scaffold					670:677	a composite polysaccharid scaffold	644:677	a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively	644:746	The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC.					
33483072	6	3	theme	anti-leakage	907:918	arg1	test					920:923	anti-leakage test	907:923	anti-leakage test	907:923	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	6	4	theme	proliferation	949:961	arg1	terms					857:861	terms	857:861	terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment	857:984	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	6	5	theme	test	920:923	arg1	terms					857:861	terms	857:861	terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment	857:984	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	1	6	theme	timely	131:136	arg1	repair					152:157	timely and effective repair	131:157	timely and effective repair of dura mater	131:171	In the field of neurosurgery, timely and effective repair of dura mater plays an important role in stabilizing the physiological functions of the human body.					
33483072	1	7	theme	physiological	216:228	arg1	functions					230:238	the physiological functions	212:238	the physiological functions of the human body	212:256	In the field of neurosurgery, timely and effective repair of dura mater plays an important role in stabilizing the physiological functions of the human body.					
33483072	11	8	theme	peritoneal	1506:1515	arg1	environment					1517:1527	the peritoneal environment	1502:1527	the peritoneal environment of mice	1502:1535	Also, it was observed that it did not deform in the peritoneal environment of mice, and tissue inflammation was mild.					
33483072	5	9	theme	O-carboxymethyl	765:779	arg1	chitin					781:786	O-carboxymethyl chitin	765:786	O-carboxymethyl chitin (O-CMCH)	765:795	The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC.					
33483072	5	9	theme	O-carboxymethyl	765:779	arg1	O-CMCH					789:794	O-CMCH	789:794	O-CMCH	789:794	The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC.					
33483072	4	10	theme	composite	514:522	arg1	film					538:541	a composite polysaccharid film	512:541	a composite polysaccharid film	512:541	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	4	10	theme	composite	514:522	arg1	layer					505:509	The dense layer	495:509	The dense layer	495:509	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	5	11	theme	porous	630:635	arg1	layer					637:641	The porous layer	626:641	The porous layer	626:641	The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC.					
33483072	5	11	theme	porous	630:635	arg1	scaffold					670:677	a composite polysaccharid scaffold	644:677	a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively	644:746	The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC.					
33483072	7	12	dep	dural	1023:1027	arg1	substitutes					1029:1039	substitutes	1029:1039	substitutes	1029:1039	Results indicated that all prepared dural substitutes were tightly bound between layers without excessively large cavities.					
33483072	3	13	theme	tissue	474:479	arg1	regeneration					481:492	tissue regeneration	474:492	tissue regeneration	474:492	It consists of a dense layer that prevents cerebrospinal fluid leakage and a porous layer that promotes tissue regeneration.					
33483072	6	14	theme	animal	968:973	arg1	experiment					975:984	animal experiment	968:984	animal experiment	968:984	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	11	15	theme	tissue	1542:1547	arg1	inflammation					1549:1560	tissue inflammation	1542:1560	tissue inflammation	1542:1560	Also, it was observed that it did not deform in the peritoneal environment of mice, and tissue inflammation was mild.					
33483072	10	16	theme	cytotoxicity	1351:1362	arg1	effect					1364:1369	no cytotoxicity effect	1348:1369	no cytotoxicity effect on NIH/3T3 cells	1348:1386	Furthermore, no leakage was observed during testing, no cytotoxicity effect on NIH/3T3 cells, and exhibited excellent cell proliferation promoting properties.					
33483072	10	16	theme	cytotoxicity	1351:1362	arg1	testing					1339:1345	testing	1339:1345	testing	1339:1345	Furthermore, no leakage was observed during testing, no cytotoxicity effect on NIH/3T3 cells, and exhibited excellent cell proliferation promoting properties.					
33483072	2	17	theme	dural	342:346	arg1	candidate					359:367	a dural substitute candidate	340:367	a dural substitute candidate	340:367	Therefore, the aim of this study is to develop a new type of bilayer membrane as a dural substitute candidate.					
33483072	2	17	theme	dural	342:346	arg1	type					312:315	a new type	306:315	a new type of bilayer membrane	306:335	Therefore, the aim of this study is to develop a new type of bilayer membrane as a dural substitute candidate.					
33483072	6	18	theme	swelling	892:899	arg1	rate					901:904	swelling rate	892:904	swelling rate	892:904	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	2	19	theme	membrane	328:335	arg1	type					312:315	a new type	306:315	a new type of bilayer membrane	306:335	Therefore, the aim of this study is to develop a new type of bilayer membrane as a dural substitute candidate.					
33483072	2	19	theme	membrane	328:335	arg1	candidate					359:367	a dural substitute candidate	340:367	a dural substitute candidate	340:367	Therefore, the aim of this study is to develop a new type of bilayer membrane as a dural substitute candidate.					
33483072	0	20	theme	bacterial	80:88	arg1	cellulose					90:98	bacterial cellulose	80:98	bacterial cellulose	80:98	A new type of bilayer dural substitute candidate made up of modified chitin and bacterial cellulose.					
33483072	11	21	theme	mice	1532:1535	arg1	environment					1517:1527	the peritoneal environment	1502:1527	the peritoneal environment of mice	1502:1535	Also, it was observed that it did not deform in the peritoneal environment of mice, and tissue inflammation was mild.					
33483072	2	22	dep	candidate	359:367	arg1	substitute					348:357	substitute	348:357	substitute	348:357	Therefore, the aim of this study is to develop a new type of bilayer membrane as a dural substitute candidate.					
33483072	10	23	from	effect	1364:1369	arg1	cells					1382:1386	NIH/3T3 cells	1374:1386	NIH/3T3 cells	1374:1386	Furthermore, no leakage was observed during testing, no cytotoxicity effect on NIH/3T3 cells, and exhibited excellent cell proliferation promoting properties.					
33483072	1	24	theme	effective	142:150	arg1	repair					152:157	timely and effective repair	131:157	timely and effective repair of dura mater	131:171	In the field of neurosurgery, timely and effective repair of dura mater plays an important role in stabilizing the physiological functions of the human body.					
33483072	0	25	theme	new	2:4	arg1	type					6:9	A new type	0:9	A new type of bilayer dural	0:26	A new type of bilayer dural substitute candidate made up of modified chitin and bacterial cellulose.					
33483072	7	26	theme	large	1095:1099	arg1	cavities					1101:1108	excessively large cavities	1083:1108	excessively large cavities	1083:1108	Results indicated that all prepared dural substitutes were tightly bound between layers without excessively large cavities.					
33483072	6	27	theme	cytotoxicity	935:946	arg1	terms					857:861	terms	857:861	terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment	857:984	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	4	28	theme	weight	575:580	arg1	chitosan					582:589	molecular weight chitosan	565:589	molecular weight chitosan (CS)	565:594	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	4	28	theme	weight	575:580	arg1	CS					592:593	CS	592:593	CS	592:593	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	6	29	dep	in	926:927	arg1	vitro					929:933	vitro	929:933	vitro	929:933	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	6	30	theme	in	926:927	arg1	cytotoxicity					935:946	in vitro cytotoxicity	926:946	in vitro cytotoxicity	926:946	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	8	31	theme	porous	1115:1120	arg1	layer					1122:1126	The porous layer	1111:1126	The porous layer	1111:1126	The porous layer showed appropriate pore size (90～200 μm) with high porous connectivity.					
33483072	8	32	theme	appropriate	1135:1145	arg1	size					1152:1155	appropriate pore size	1135:1155	appropriate pore size (90～200 μm)	1135:1167	The porous layer showed appropriate pore size (90～200 μm) with high porous connectivity.					
33483072	8	32	theme	appropriate	1135:1145	arg1	μm					1165:1166	90～200 μm	1158:1166	90～200 μm	1158:1166	The porous layer showed appropriate pore size (90～200 μm) with high porous connectivity.					
33483072	1	33	theme	human	247:251	arg1	body					253:256	the human body	243:256	the human body	243:256	In the field of neurosurgery, timely and effective repair of dura mater plays an important role in stabilizing the physiological functions of the human body.					
33483072	8	34	theme	high	1174:1177	arg1	connectivity					1186:1197	high porous connectivity	1174:1197	high porous connectivity	1174:1197	The porous layer showed appropriate pore size (90～200 μm) with high porous connectivity.					
33483072	1	35	theme	dura	162:165	arg1	mater					167:171	dura mater	162:171	dura mater	162:171	In the field of neurosurgery, timely and effective repair of dura mater plays an important role in stabilizing the physiological functions of the human body.					
33483072	5	36	theme	citric	718:723	arg1	CA					731:732	CA	731:732	CA	731:732	The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC.					
33483072	5	36	theme	citric	718:723	arg1	acid					725:728	citric acid	718:728	citric acid (CA)	718:733	The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC.					
33483072	1	37	theme	body	253:256	arg1	functions					230:238	the physiological functions	212:238	the physiological functions of the human body	212:256	In the field of neurosurgery, timely and effective repair of dura mater plays an important role in stabilizing the physiological functions of the human body.					
33483072	8	38	theme	pore	1147:1150	arg1	size					1152:1155	appropriate pore size	1135:1155	appropriate pore size (90～200 μm)	1135:1167	The porous layer showed appropriate pore size (90～200 μm) with high porous connectivity.					
33483072	8	38	theme	pore	1147:1150	arg1	μm					1165:1166	90～200 μm	1158:1166	90～200 μm	1158:1166	The porous layer showed appropriate pore size (90～200 μm) with high porous connectivity.					
33483072	4	39	theme	bacterial	600:608	arg1	BC					621:622	BC	621:622	BC	621:622	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	4	39	theme	bacterial	600:608	arg1	cellulose					610:618	bacterial cellulose	600:618	bacterial cellulose (BC)	600:623	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	1	40	theme	mater	167:171	arg1	repair					152:157	timely and effective repair	131:157	timely and effective repair of dura mater	131:171	In the field of neurosurgery, timely and effective repair of dura mater plays an important role in stabilizing the physiological functions of the human body.					
33483072	0	41	theme	dural	22:26	arg1	bilayer					14:20	bilayer dural	14:26	bilayer dural	14:26	A new type of bilayer dural substitute candidate made up of modified chitin and bacterial cellulose.					
33483072	3	42	theme	fluid	427:431	arg1	leakage					433:439	cerebrospinal fluid leakage	413:439	cerebrospinal fluid leakage	413:439	It consists of a dense layer that prevents cerebrospinal fluid leakage and a porous layer that promotes tissue regeneration.					
33483072	9	43	dep	dural	1222:1226	arg1	substitutes					1228:1238	substitutes	1228:1238	substitutes	1228:1238	The optimized bilayer dural substitutes showed suitable swelling rate and mechanical behavior.					
33483072	4	44	theme	molecular	565:573	arg1	chitosan					582:589	molecular weight chitosan	565:589	molecular weight chitosan (CS)	565:594	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	4	44	theme	molecular	565:573	arg1	CS					592:593	CS	592:593	CS	592:593	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	0	45	theme	bilayer	14:20	arg1	type					6:9	A new type	0:9	A new type of bilayer dural	0:26	A new type of bilayer dural substitute candidate made up of modified chitin and bacterial cellulose.					
33483072	6	46	theme	SEM	866:868	arg1	terms					857:861	terms	857:861	terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment	857:984	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	6	47	dep	dural	817:821	arg1	substitutes					823:833	substitutes	823:833	substitutes	823:833	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	9	48	theme	mechanical	1274:1283	arg1	behavior					1285:1292	mechanical behavior	1274:1292	mechanical behavior	1274:1292	The optimized bilayer dural substitutes showed suitable swelling rate and mechanical behavior.					
33483072	4	49	theme	high	560:563	arg1	chitosan					582:589	molecular weight chitosan	565:589	molecular weight chitosan (CS)	565:594	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	4	49	theme	high	560:563	arg1	CS					592:593	CS	592:593	CS	592:593	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	10	50	theme	excellent	1403:1411	arg1	proliferation					1418:1430	excellent cell proliferation promoting	1403:1440	excellent cell proliferation promoting properties	1403:1451	Furthermore, no leakage was observed during testing, no cytotoxicity effect on NIH/3T3 cells, and exhibited excellent cell proliferation promoting properties.					
33483072	10	51	theme	promoting	1432:1440	arg1	proliferation					1418:1430	excellent cell proliferation promoting	1403:1440	excellent cell proliferation promoting properties	1403:1451	Furthermore, no leakage was observed during testing, no cytotoxicity effect on NIH/3T3 cells, and exhibited excellent cell proliferation promoting properties.					
33483072	10	52	theme	cell	1413:1416	arg1	proliferation					1418:1430	excellent cell proliferation promoting	1403:1440	excellent cell proliferation promoting properties	1403:1451	Furthermore, no leakage was observed during testing, no cytotoxicity effect on NIH/3T3 cells, and exhibited excellent cell proliferation promoting properties.					
33483072	3	53	theme	cerebrospinal	413:425	arg1	leakage					433:439	cerebrospinal fluid leakage	413:439	cerebrospinal fluid leakage	413:439	It consists of a dense layer that prevents cerebrospinal fluid leakage and a porous layer that promotes tissue regeneration.					
33483072	9	54	theme	suitable	1247:1254	arg1	rate					1265:1268	suitable swelling rate	1247:1268	suitable swelling rate	1247:1268	The optimized bilayer dural substitutes showed suitable swelling rate and mechanical behavior.					
33483072	10	55	theme	proliferation	1418:1430	arg1	properties					1442:1451	excellent cell proliferation promoting properties	1403:1451	excellent cell proliferation promoting properties	1403:1451	Furthermore, no leakage was observed during testing, no cytotoxicity effect on NIH/3T3 cells, and exhibited excellent cell proliferation promoting properties.					
33483072	2	56	theme	study	286:290	arg1	aim					274:276	the aim	270:276	the aim of this study	270:290	Therefore, the aim of this study is to develop a new type of bilayer membrane as a dural substitute candidate.					
33483072	9	57	theme	swelling	1256:1263	arg1	rate					1265:1268	suitable swelling rate	1247:1268	suitable swelling rate	1247:1268	The optimized bilayer dural substitutes showed suitable swelling rate and mechanical behavior.					
33483072	6	58	theme	experiment	975:984	arg1	terms					857:861	terms	857:861	terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment	857:984	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	1	59	theme	important	182:190	arg1	role					192:195	an important role	179:195	an important role	179:195	In the field of neurosurgery, timely and effective repair of dura mater plays an important role in stabilizing the physiological functions of the human body.					
33483072	8	60	theme	porous	1179:1184	arg1	connectivity					1186:1197	high porous connectivity	1174:1197	high porous connectivity	1174:1197	The porous layer showed appropriate pore size (90～200 μm) with high porous connectivity.					
33483072	3	61	theme	porous	447:452	arg1	layer					454:458	a porous layer	445:458	a porous layer that promotes tissue regeneration	445:492	It consists of a dense layer that prevents cerebrospinal fluid leakage and a porous layer that promotes tissue regeneration.					
33483072	4	62	theme	polysaccharid	524:536	arg1	film					538:541	a composite polysaccharid film	512:541	a composite polysaccharid film	512:541	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	4	62	theme	polysaccharid	524:536	arg1	layer					505:509	The dense layer	495:509	The dense layer	495:509	The dense layer, a composite polysaccharid film, was composed of high molecular weight chitosan (CS) and bacterial cellulose (BC).					
33483072	10	63	theme	NIH/3T3	1374:1380	arg1	cells					1382:1386	NIH/3T3 cells	1374:1386	NIH/3T3 cells	1374:1386	Furthermore, no leakage was observed during testing, no cytotoxicity effect on NIH/3T3 cells, and exhibited excellent cell proliferation promoting properties.					
33483072	6	64	theme	rate	901:904	arg1	terms					857:861	terms	857:861	terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment	857:984	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	3	65	theme	dense	387:391	arg1	layer					393:397	a dense layer	385:397	a dense layer that prevents cerebrospinal fluid leakage	385:439	It consists of a dense layer that prevents cerebrospinal fluid leakage and a porous layer that promotes tissue regeneration.					
33483072	2	66	theme	new	308:310	arg1	type					312:315	a new type	306:315	a new type of bilayer membrane	306:335	Therefore, the aim of this study is to develop a new type of bilayer membrane as a dural substitute candidate.					
33483072	2	66	theme	new	308:310	arg1	candidate					359:367	a dural substitute candidate	340:367	a dural substitute candidate	340:367	Therefore, the aim of this study is to develop a new type of bilayer membrane as a dural substitute candidate.					
33483072	2	67	theme	bilayer	320:326	arg1	membrane					328:335	bilayer membrane	320:335	bilayer membrane	320:335	Therefore, the aim of this study is to develop a new type of bilayer membrane as a dural substitute candidate.					
33483072	6	68	theme	behavior	882:889	arg1	terms					857:861	terms	857:861	terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment	857:984	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
33483072	5	69	theme	composite	646:654	arg1	layer					637:641	The porous layer	626:641	The porous layer	626:641	The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC.					
33483072	5	69	theme	composite	646:654	arg1	scaffold					670:677	a composite polysaccharid scaffold	644:677	a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively	644:746	The porous layer, a composite polysaccharid scaffold cross-linked by glutaraldehyde (GA) or citric acid (CA) respectively, was composed of O-carboxymethyl chitin (O-CMCH) and BC.					
33483072	1	70	theme	neurosurgery	117:128	arg1	field					108:112	the field	104:112	the field of neurosurgery	104:128	In the field of neurosurgery, timely and effective repair of dura mater plays an important role in stabilizing the physiological functions of the human body.					
33483072	6	71	theme	mechanical	871:880	arg1	behavior					882:889	mechanical behavior	871:889	mechanical behavior	871:889	The bilayer dural substitutes were characterized in terms of SEM, mechanical behavior, swelling rate, anti-leakage test, in vitro cytotoxicity, proliferation, and animal experiment.					
32029691	9	0	theme	hydrogel	1696:1703	arg1	hydrogels					1685:1693	bulk hydrogels	1680:1693	bulk hydrogels (hydrogel inks)	1680:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	9	0	theme	hydrogel	1696:1703	arg1	inks					1705:1708	hydrogel inks	1696:1708	hydrogel inks	1696:1708	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	1	1	with	matrix	260:265	arg1	properties					278:287	properties	278:287	properties similar to that of native tissue	278:320	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	3	2	theme	cell-free	532:540	arg1	ink					566:568	cell-free or cell-loaded hydrogel ink	532:568	cell-free or cell-loaded hydrogel ink	532:568	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	1	3	theme	similar	289:295	arg1	properties					278:287	properties	278:287	properties similar to that of native tissue	278:320	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	7	4	theme	shear-thinning	1360:1373	arg1	behavior					1375:1382	shear-thinning behavior	1360:1382	shear-thinning behavior of the hydrogel inks	1360:1403	The incorporation of cCNCs and XG manipulated the flow and shear-thinning behavior of the hydrogel inks, thereby improving the printing ability.					
32029691	3	5	theme	ink	566:568	arg1	printing					520:527	Extrusion 3D printing	507:527	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink	507:568	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	1	6	theme	great	187:191	arg1	challenge					193:201	a great challenge	185:201	a great challenge	185:201	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	2	7	from	ECM	378:380	arg1	regeneration					389:400	the regeneration	385:400	the regeneration of damaged tissues	385:419	Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM.					
32029691	4	8	theme	stable	716:721	arg1	inks					758:761	stable cell-free and cell-loaded hydrogel inks	716:761	stable cell-free and cell-loaded hydrogel inks	716:761	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	6	9	theme	matrix	1293:1298	arg1	behavior					1247:1254	the rheological behavior	1231:1254	the rheological behavior of the sodium alginate (SA) hydrogel matrix	1231:1298	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	2	10	theme	Polysaccharide-based	323:342	arg1	hydrogel					344:351	Polysaccharide-based hydrogel	323:351	Polysaccharide-based hydrogel	323:351	Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM.					
32029691	9	11	theme	human	1637:1641	arg1	cells					1671:1675	the human skin fibroblast (CCD-986Sk) cells	1633:1675	the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks)	1633:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	9	12	theme	qualitative	1749:1759	arg1	analyses					1783:1790	both qualitative and quantitative cell analyses	1744:1790	both qualitative and quantitative cell analyses	1744:1790	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	8	13	theme	post-printing	1494:1506	arg1	fidelity					1508:1515	post-printing fidelity	1494:1515	post-printing fidelity	1494:1515	The results showed good rheological properties, post-printing fidelity, and dynamic mechanical properties under compression of the developed hydrogel inks.					
32029691	7	14	theme	printing	1428:1435	arg1	ability					1437:1443	the printing ability	1424:1443	the printing ability	1424:1443	The incorporation of cCNCs and XG manipulated the flow and shear-thinning behavior of the hydrogel inks, thereby improving the printing ability.					
32029691	3	15	theme	desired	621:627	arg1	compositions					629:640	desired compositions	621:640	desired compositions	621:640	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	1	16	theme	artificial	235:244	arg1	ECM					268:270	ECM	268:270	ECM	268:270	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	1	16	theme	artificial	235:244	arg1	matrix					260:265	artificial extracellular matrix	235:265	artificial extracellular matrix (ECM) with properties similar to that of native tissue	235:320	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	9	17	theme	quantitative	1765:1776	arg1	analyses					1783:1790	both qualitative and quantitative cell analyses	1744:1790	both qualitative and quantitative cell analyses	1744:1790	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	6	18	theme	SA	1280:1281	arg1	matrix					1293:1298	the sodium alginate (SA) hydrogel matrix	1259:1298	the sodium alginate (SA) hydrogel matrix	1259:1298	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	10	19	theme	hydrogel	1823:1830	arg1	inks					1832:1835	SA hydrogel inks	1820:1835	SA hydrogel inks	1820:1835	The use of cCNCs and XG in SA hydrogel inks provides a primary insight for further improvement in designing 3D bioprintable hydrogel inks.					
32029691	10	20	from	use	1797:1799	arg1	inks					1832:1835	SA hydrogel inks	1820:1835	SA hydrogel inks	1820:1835	The use of cCNCs and XG in SA hydrogel inks provides a primary insight for further improvement in designing 3D bioprintable hydrogel inks.					
32029691	8	21	theme	dynamic	1522:1528	arg1	properties					1541:1550	dynamic mechanical properties	1522:1550	dynamic mechanical properties	1522:1550	The results showed good rheological properties, post-printing fidelity, and dynamic mechanical properties under compression of the developed hydrogel inks.					
32029691	6	22	theme	alginate	1270:1277	arg1	matrix					1293:1298	the sodium alginate (SA) hydrogel matrix	1259:1298	the sodium alginate (SA) hydrogel matrix	1259:1298	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	4	23	theme	physicochemical	776:790	arg1	properties					792:801	optimal physicochemical properties	768:801	optimal physicochemical properties	768:801	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	9	24	theme	skin	1643:1646	arg1	cells					1671:1675	the human skin fibroblast (CCD-986Sk) cells	1633:1675	the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks)	1633:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	5	25	theme	transmission	999:1010	arg1	microscopy					1021:1030	transmission electron microscopy	999:1030	transmission electron microscopy	999:1030	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	7	26	theme	hydrogel	1391:1398	arg1	inks					1400:1403	the hydrogel inks	1387:1403	the hydrogel inks	1387:1403	The incorporation of cCNCs and XG manipulated the flow and shear-thinning behavior of the hydrogel inks, thereby improving the printing ability.					
32029691	1	27	dep	three-dimensional	110:126	arg1	3D					129:130	3D	129:130	3D	129:130	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	0	28	theme	tissue	77:82	arg1	engineering					84:94	tissue engineering	77:94	tissue engineering	77:94	3D printable carboxylated cellulose nanocrystal-reinforced hydrogel inks for tissue engineering.					
32029691	10	29	theme	primary	1848:1854	arg1	insight					1856:1862	a primary insight	1846:1862	a primary insight for further improvement in designing 3D bioprintable hydrogel inks	1846:1929	The use of cCNCs and XG in SA hydrogel inks provides a primary insight for further improvement in designing 3D bioprintable hydrogel inks.					
32029691	1	30	theme	tissue	162:167	arg1	regeneration					169:180	complex tissue regeneration	154:180	complex tissue regeneration	154:180	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	9	31	from	viability	1620:1628	arg1	hydrogels					1685:1693	bulk hydrogels	1680:1693	bulk hydrogels (hydrogel inks)	1680:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	9	31	from	viability	1620:1628	arg1	inks					1705:1708	hydrogel inks	1696:1708	hydrogel inks	1696:1708	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	4	32	theme	hydrogel	749:756	arg1	inks					758:761	stable cell-free and cell-loaded hydrogel inks	716:761	stable cell-free and cell-loaded hydrogel inks	716:761	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	4	33	from	concern	840:846	arg1	printing					883:890	direct-write extrusion-based 3D printing	851:890	direct-write extrusion-based 3D printing	851:890	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	5	34	theme	ammonium	972:979	arg1	persulfate					981:990	ammonium persulfate	972:990	ammonium persulfate	972:990	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	9	35	theme	fibroblast	1648:1657	arg1	cells					1671:1675	the human skin fibroblast (CCD-986Sk) cells	1633:1675	the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks)	1633:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	5	36	theme	spectroscopy	1060:1071	arg1	analyses					1096:1103	transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses	999:1103	transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses	999:1103	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	9	37	from	hydrogels	1685:1693	arg1	viability					1620:1628	good viability	1615:1628	good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks)	1615:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	8	38	theme	hydrogel	1587:1594	arg1	inks					1596:1599	the developed hydrogel inks	1573:1599	the developed hydrogel inks	1573:1599	The results showed good rheological properties, post-printing fidelity, and dynamic mechanical properties under compression of the developed hydrogel inks.					
32029691	2	39	theme	damaged	405:411	arg1	tissues					413:419	damaged tissues	405:419	damaged tissues	405:419	Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM.					
32029691	0	40	theme	3D	0:1	arg1	printable					3:11	3D printable	0:11	3D printable	0:11	3D printable carboxylated cellulose nanocrystal-reinforced hydrogel inks for tissue engineering.					
32029691	5	41	theme	microscopy	1021:1030	arg1	analyses					1096:1103	transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses	999:1103	transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses	999:1103	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	5	42	theme	Fourier-transform	1033:1049	arg1	spectroscopy					1060:1071	Fourier-transform infrared spectroscopy	1033:1071	Fourier-transform infrared spectroscopy	1033:1071	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	0	43	theme	nanocrystal-reinforced	36:57	arg1	inks					68:71	cellulose nanocrystal-reinforced hydrogel inks	26:71	cellulose nanocrystal-reinforced hydrogel inks for tissue engineering	26:94	3D printable carboxylated cellulose nanocrystal-reinforced hydrogel inks for tissue engineering.					
32029691	5	44	theme	successful	1121:1130	arg1	preparation					1132:1142	their successful preparation	1115:1142	their successful preparation	1115:1142	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	4	45	with	development	701:711	arg1	biocompatibility					807:822	biocompatibility	807:822	biocompatibility	807:822	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	4	45	with	development	701:711	arg1	properties					792:801	optimal physicochemical properties	768:801	optimal physicochemical properties	768:801	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	3	46	theme	engineering	671:681	arg1	applications					683:694	tissue engineering applications	664:694	tissue engineering applications	664:694	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	3	47	theme	3D	517:518	arg1	printing					520:527	Extrusion 3D printing	507:527	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink	507:568	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	10	48	theme	further	1868:1874	arg1	improvement					1876:1886	further improvement	1868:1886	further improvement in designing 3D bioprintable hydrogel inks	1868:1929	The use of cCNCs and XG in SA hydrogel inks provides a primary insight for further improvement in designing 3D bioprintable hydrogel inks.					
32029691	9	49	theme	cells	1671:1675	arg1	viability					1620:1628	good viability	1615:1628	good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks)	1615:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	7	50	theme	XG	1332:1333	arg1	incorporation					1305:1317	The incorporation	1301:1317	The incorporation of cCNCs and XG	1301:1333	The incorporation of cCNCs and XG manipulated the flow and shear-thinning behavior of the hydrogel inks, thereby improving the printing ability.					
32029691	8	51	theme	rheological	1470:1480	arg1	properties					1482:1491	good rheological properties	1465:1491	good rheological properties	1465:1491	The results showed good rheological properties, post-printing fidelity, and dynamic mechanical properties under compression of the developed hydrogel inks.					
32029691	9	52	theme	CCD-986Sk	1660:1668	arg1	cells					1671:1675	the human skin fibroblast (CCD-986Sk) cells	1633:1675	the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks)	1633:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	2	53	theme	great	359:363	arg1	potential					365:373	great potential	359:373	great potential as ECM in the regeneration of damaged tissues or reconstruction of organs	359:447	Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM.					
32029691	1	54	theme	extracellular	246:258	arg1	ECM					268:270	ECM	268:270	ECM	268:270	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	1	54	theme	extracellular	246:258	arg1	matrix					260:265	artificial extracellular matrix	235:265	artificial extracellular matrix (ECM) with properties similar to that of native tissue	235:320	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	5	55	theme	diffraction	1084:1094	arg1	analyses					1096:1103	transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses	999:1103	transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses	999:1103	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	7	56	theme	cCNCs	1322:1326	arg1	incorporation					1305:1317	The incorporation	1301:1317	The incorporation of cCNCs and XG	1301:1333	The incorporation of cCNCs and XG manipulated the flow and shear-thinning behavior of the hydrogel inks, thereby improving the printing ability.					
32029691	10	57	theme	bioprintable	1904:1915	arg1	inks					1926:1929	3D bioprintable hydrogel inks	1901:1929	3D bioprintable hydrogel inks	1901:1929	The use of cCNCs and XG in SA hydrogel inks provides a primary insight for further improvement in designing 3D bioprintable hydrogel inks.					
32029691	4	58	theme	3D	880:881	arg1	printing					883:890	direct-write extrusion-based 3D printing	851:890	direct-write extrusion-based 3D printing	851:890	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	6	59	theme	xanthan	1189:1195	arg1	XG					1202:1203	XG	1202:1203	XG	1202:1203	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	6	59	theme	xanthan	1189:1195	arg1	-COOH					1207:1211	-COOH	1207:1211	-COOH	1207:1211	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	6	59	theme	xanthan	1189:1195	arg1	gum					1197:1199	xanthan gum	1189:1199	xanthan gum (XG) (-COOH)	1189:1212	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	10	60	theme	cCNCs	1804:1808	arg1	use					1797:1799	The use	1793:1799	The use of cCNCs and XG in SA hydrogel inks	1793:1835	The use of cCNCs and XG in SA hydrogel inks provides a primary insight for further improvement in designing 3D bioprintable hydrogel inks.					
32029691	6	61	theme	rheological	1235:1245	arg1	behavior					1247:1254	the rheological behavior	1231:1254	the rheological behavior of the sodium alginate (SA) hydrogel matrix	1231:1298	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	3	62	theme	hydrogel	557:564	arg1	ink					566:568	cell-free or cell-loaded hydrogel ink	532:568	cell-free or cell-loaded hydrogel ink	532:568	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	10	63	theme	SA	1820:1821	arg1	inks					1832:1835	SA hydrogel inks	1820:1835	SA hydrogel inks	1820:1835	The use of cCNCs and XG in SA hydrogel inks provides a primary insight for further improvement in designing 3D bioprintable hydrogel inks.					
32029691	10	64	theme	XG	1814:1815	arg1	use					1797:1799	The use	1793:1799	The use of cCNCs and XG in SA hydrogel inks	1793:1835	The use of cCNCs and XG in SA hydrogel inks provides a primary insight for further improvement in designing 3D bioprintable hydrogel inks.					
32029691	3	65	dep	compositions	629:640	arg1	the					617:619	the	617:619	the	617:619	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	3	66	theme	sophisticated	588:600	arg1	fabrication					602:612	a more sophisticated fabrication	581:612	a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications	581:694	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	5	67	theme	carboxylated	908:919	arg1	cCNCs					945:949	cCNCs	945:949	cCNCs	945:949	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	5	67	theme	carboxylated	908:919	arg1	nanocrystals					931:942	carboxylated cellulose nanocrystals	908:942	carboxylated cellulose nanocrystals (cCNCs)	908:950	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	9	68	from	cells	1671:1675	arg1	hydrogels					1685:1693	bulk hydrogels	1680:1693	bulk hydrogels (hydrogel inks)	1680:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	9	68	from	cells	1671:1675	arg1	inks					1705:1708	hydrogel inks	1696:1708	hydrogel inks	1696:1708	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	9	69	theme	cell	1778:1781	arg1	analyses					1783:1790	both qualitative and quantitative cell analyses	1744:1790	both qualitative and quantitative cell analyses	1744:1790	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	8	70	theme	mechanical	1530:1539	arg1	properties					1541:1550	dynamic mechanical properties	1522:1550	dynamic mechanical properties	1522:1550	The results showed good rheological properties, post-printing fidelity, and dynamic mechanical properties under compression of the developed hydrogel inks.					
32029691	4	71	theme	inks	758:761	arg1	development					701:711	The development	697:711	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility	697:822	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	4	71	theme	inks	758:761	arg1	concern					840:846	a major concern	832:846	a major concern in direct-write extrusion-based 3D printing	832:890	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	4	72	theme	direct-write	851:862	arg1	printing					883:890	direct-write extrusion-based 3D printing	851:890	direct-write extrusion-based 3D printing	851:890	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	3	73	theme	architectures	646:658	arg1	fabrication					602:612	a more sophisticated fabrication	581:612	a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications	581:694	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	6	74	theme	sodium	1263:1268	arg1	matrix					1293:1298	the sodium alginate (SA) hydrogel matrix	1259:1298	the sodium alginate (SA) hydrogel matrix	1259:1298	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	4	75	theme	optimal	768:774	arg1	properties					792:801	optimal physicochemical properties	768:801	optimal physicochemical properties	768:801	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	3	76	theme	compositions	629:640	arg1	fabrication					602:612	a more sophisticated fabrication	581:612	a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications	581:694	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	1	77	theme	complex	154:160	arg1	regeneration					169:180	complex tissue regeneration	154:180	complex tissue regeneration	154:180	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	7	78	theme	inks	1400:1403	arg1	flow					1351:1354	the flow	1347:1354	the flow	1347:1354	The incorporation of cCNCs and XG manipulated the flow and shear-thinning behavior of the hydrogel inks, thereby improving the printing ability.					
32029691	7	78	theme	inks	1400:1403	arg1	behavior					1375:1382	shear-thinning behavior	1360:1382	shear-thinning behavior of the hydrogel inks	1360:1403	The incorporation of cCNCs and XG manipulated the flow and shear-thinning behavior of the hydrogel inks, thereby improving the printing ability.					
32029691	4	79	theme	cell-free	723:731	arg1	inks					758:761	stable cell-free and cell-loaded hydrogel inks	716:761	stable cell-free and cell-loaded hydrogel inks	716:761	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	8	80	theme	developed	1577:1585	arg1	inks					1596:1599	the developed hydrogel inks	1573:1599	the developed hydrogel inks	1573:1599	The results showed good rheological properties, post-printing fidelity, and dynamic mechanical properties under compression of the developed hydrogel inks.					
32029691	4	81	theme	cell-loaded	737:747	arg1	inks					758:761	stable cell-free and cell-loaded hydrogel inks	716:761	stable cell-free and cell-loaded hydrogel inks	716:761	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	9	82	theme	bulk	1680:1683	arg1	hydrogels					1685:1693	bulk hydrogels	1680:1693	bulk hydrogels (hydrogel inks)	1680:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	9	82	theme	bulk	1680:1683	arg1	inks					1705:1708	hydrogel inks	1696:1708	hydrogel inks	1696:1708	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	5	83	theme	infrared	1051:1058	arg1	spectroscopy					1060:1071	Fourier-transform infrared spectroscopy	1033:1071	Fourier-transform infrared spectroscopy	1033:1071	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	8	84	theme	inks	1596:1599	arg1	compression					1558:1568	compression	1558:1568	compression of the developed hydrogel inks	1558:1599	The results showed good rheological properties, post-printing fidelity, and dynamic mechanical properties under compression of the developed hydrogel inks.					
32029691	9	85	theme	good	1615:1618	arg1	viability					1620:1628	good viability	1615:1628	good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks)	1615:1709	Furthermore, good viability of the human skin fibroblast (CCD-986Sk) cells on bulk hydrogels (hydrogel inks) was observed, as demonstrated by both qualitative and quantitative cell analyses.					
32029691	4	86	theme	major	834:838	arg1	development					701:711	The development	697:711	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility	697:822	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	4	86	theme	major	834:838	arg1	concern					840:846	a major concern	832:846	a major concern in direct-write extrusion-based 3D printing	832:890	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	2	87	theme	tissues	413:419	arg1	regeneration					389:400	the regeneration	385:400	the regeneration of damaged tissues	385:419	Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM.					
32029691	5	88	theme	electron	1012:1019	arg1	microscopy					1021:1030	transmission electron microscopy	999:1030	transmission electron microscopy	999:1030	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	6	89	theme	cCNCs	1168:1172	arg1	effect					1158:1163	the effect	1154:1163	the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH)	1154:1212	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	2	90	theme	similar	475:481	arg1	properties					464:473	properties	464:473	properties similar to those of native ECM	464:504	Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM.					
32029691	0	91	theme	cellulose	26:34	arg1	inks					68:71	cellulose nanocrystal-reinforced hydrogel inks	26:71	cellulose nanocrystal-reinforced hydrogel inks for tissue engineering	26:94	3D printable carboxylated cellulose nanocrystal-reinforced hydrogel inks for tissue engineering.					
32029691	1	92	theme	three-dimensional	110:126	arg1	microenvironment					133:148	a three-dimensional (3D) microenvironment	108:148	a three-dimensional (3D) microenvironment for complex tissue regeneration	108:180	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	6	93	theme	hydrogel	1284:1291	arg1	matrix					1293:1298	the sodium alginate (SA) hydrogel matrix	1259:1298	the sodium alginate (SA) hydrogel matrix	1259:1298	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	10	94	theme	3D	1901:1902	arg1	inks					1926:1929	3D bioprintable hydrogel inks	1901:1929	3D bioprintable hydrogel inks	1901:1929	The use of cCNCs and XG in SA hydrogel inks provides a primary insight for further improvement in designing 3D bioprintable hydrogel inks.					
32029691	2	95	theme	organs	442:447	arg1	reconstruction					424:437	reconstruction	424:437	reconstruction of organs	424:447	Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM.					
32029691	2	95	theme	organs	442:447	arg1	ECM					378:380	ECM	378:380	ECM in the regeneration of damaged tissues	378:419	Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM.					
32029691	0	96	theme	hydrogel	59:66	arg1	inks					68:71	cellulose nanocrystal-reinforced hydrogel inks	26:71	cellulose nanocrystal-reinforced hydrogel inks for tissue engineering	26:94	3D printable carboxylated cellulose nanocrystal-reinforced hydrogel inks for tissue engineering.					
32029691	1	97	theme	native	308:313	arg1	tissue					315:320	native tissue	308:320	native tissue	308:320	To achieve a three-dimensional (3D) microenvironment for complex tissue regeneration is a great challenge when developing biomaterials as artificial extracellular matrix (ECM) with properties similar to that of native tissue.					
32029691	2	98	theme	native	495:500	arg1	ECM					502:504	native ECM	495:504	native ECM	495:504	Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM.					
32029691	3	99	theme	tissue	664:669	arg1	applications					683:694	tissue engineering applications	664:694	tissue engineering applications	664:694	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	5	100	theme	x-ray	1078:1082	arg1	diffraction					1084:1094	x-ray diffraction	1078:1094	x-ray diffraction	1078:1094	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	10	101	theme	hydrogel	1917:1924	arg1	inks					1926:1929	3D bioprintable hydrogel inks	1901:1929	3D bioprintable hydrogel inks	1901:1929	The use of cCNCs and XG in SA hydrogel inks provides a primary insight for further improvement in designing 3D bioprintable hydrogel inks.					
32029691	8	102	theme	good	1465:1468	arg1	properties					1482:1491	good rheological properties	1465:1491	good rheological properties	1465:1491	The results showed good rheological properties, post-printing fidelity, and dynamic mechanical properties under compression of the developed hydrogel inks.					
32029691	3	103	theme	Extrusion	507:515	arg1	printing					520:527	Extrusion 3D printing	507:527	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink	507:568	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32029691	5	104	theme	cellulose	921:929	arg1	cCNCs					945:949	cCNCs	945:949	cCNCs	945:949	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	5	104	theme	cellulose	921:929	arg1	nanocrystals					931:942	carboxylated cellulose nanocrystals	908:942	carboxylated cellulose nanocrystals (cCNCs)	908:950	In this study, carboxylated cellulose nanocrystals (cCNCs) were prepared using ammonium persulfate, where transmission electron microscopy, Fourier-transform infrared spectroscopy, and x-ray diffraction analyses confirmed their successful preparation.					
32029691	2	105	from	reconstruction	424:437	arg1	regeneration					389:400	the regeneration	385:400	the regeneration of damaged tissues	385:419	Polysaccharide-based hydrogel shows great potential as ECM in the regeneration of damaged tissues or reconstruction of organs, demonstrating properties similar to those of native ECM.					
32029691	6	106	theme	gum	1197:1199	arg1	effect					1158:1163	the effect	1154:1163	the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH)	1154:1212	Further, the effect of cCNCs (-COOH) and/or xanthan gum (XG) (-COOH) was evaluated on the rheological behavior of the sodium alginate (SA) hydrogel matrix.					
32029691	4	107	theme	extrusion-based	864:878	arg1	printing					883:890	direct-write extrusion-based 3D printing	851:890	direct-write extrusion-based 3D printing	851:890	The development of stable cell-free and cell-loaded hydrogel inks with optimal physicochemical properties and biocompatibility is also a major concern in direct-write extrusion-based 3D printing.					
32029691	3	108	theme	cell-loaded	545:555	arg1	ink					566:568	cell-free or cell-loaded hydrogel ink	532:568	cell-free or cell-loaded hydrogel ink	532:568	Extrusion 3D printing of cell-free or cell-loaded hydrogel ink has led to a more sophisticated fabrication of the desired compositions and architectures for tissue engineering applications.					
32122493	3	0	theme	NF-κB	618:622	arg1	p-IκB-α					609:615	p-IκB-α	609:615	p-IκB-α	609:615	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	3	0	theme	NF-κB	618:622	arg1	p65					624:626	NF-κB p65	618:626	NF-κB p65	618:626	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	1	1	theme	protective	177:186	arg1	effect					188:193	the protective effect	173:193	the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice	173:348	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	6	2	theme	β-diversity	828:838	arg1	analysis					840:847	α-diversity and β-diversity analysis	812:847	analysis	840:847	α-diversity and β-diversity analysis revealed CSP improved microbial community diversity and modulated the overall structure of gut microbiota.					
32122493	2	3	theme	cytokines	386:394	arg1	secretion					396:404	cytokines secretion	386:404	cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3)	386:547	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	1	4	theme	-induced	272:279	arg1	immunosuppression					300:316	cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression	251:316	cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression	251:316	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	2	5	theme	factors	500:506	arg1	production					508:517	transcription factors production	486:517	transcription factors production	486:517	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	6	6	theme	α-diversity	812:822	arg1	analysis					840:847	α-diversity and β-diversity analysis	812:847	analysis	840:847	α-diversity and β-diversity analysis revealed CSP improved microbial community diversity and modulated the overall structure of gut microbiota.					
32122493	7	7	dep	Clostridium	1121:1131	arg1	Flexispira					1134:1143	Flexispira	1134:1143	Flexispira	1134:1143	Taxonomic composition analysis found that CSP increased the abundance of probiotics (Lactobacillus, Bifidobacterium, Bacteroides) and decreased pathogenic bacteria (Clostridium, Flexispira).					
32122493	1	8	from	effect	188:193	arg1	immunosuppression					300:316	cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression	251:316	cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression	251:316	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	1	8	from	effect	188:193	arg1	dysbiosis					332:340	microbial dysbiosis	322:340	microbial dysbiosis	322:340	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	2	9	dep	secretion	396:404	arg1	GATA-3					527:532	GATA-3	527:532	GATA-3	527:532	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	9	dep	secretion	396:404	arg1	RORγt					535:539	RORγt	535:539	RORγt	535:539	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	9	dep	secretion	396:404	arg1	production					508:517	transcription factors production	486:517	transcription factors production	486:517	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	9	dep	secretion	396:404	arg1	IL-21					475:479	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	IL-21	475:479	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	9	dep	secretion	396:404	arg1	T-bet					520:524	T-bet	520:524	T-bet	520:524	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	9	dep	secretion	396:404	arg1	Foxp3					542:546	Foxp3	542:546	Foxp3	542:546	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	1	10	theme	intestinal	281:290	arg1	immunosuppression					300:316	cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression	251:316	cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression	251:316	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	0	11	theme	cyclophosphamide-treated	103:126	arg1	mice					128:131	cyclophosphamide-treated mice	103:131	cyclophosphamide-treated mice	103:131	Cultured Cordyceps sinensis polysaccharides modulate intestinal mucosal immunity and gut microbiota in cyclophosphamide-treated mice.					
32122493	7	12	theme	Taxonomic	956:964	arg1	analysis					978:985	Taxonomic composition analysis	956:985	Taxonomic composition analysis	956:985	Taxonomic composition analysis found that CSP increased the abundance of probiotics (Lactobacillus, Bifidobacterium, Bacteroides) and decreased pathogenic bacteria (Clostridium, Flexispira).					
32122493	8	13	theme	gut	1273:1275	arg1	microbiota					1277:1286	gut microbiota	1273:1286	gut microbiota	1273:1286	These findings suggested the potential of CSP as a prebiotics to reduce side effects of Cy on intestinal mucosal immunity and gut microbiota.					
32122493	1	14	theme	mucosal	292:298	arg1	immunosuppression					300:316	cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression	251:316	cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression	251:316	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	8	15	from	effects	1224:1230	arg1	immunity					1260:1267	intestinal mucosal immunity	1241:1267	intestinal mucosal immunity	1241:1267	These findings suggested the potential of CSP as a prebiotics to reduce side effects of Cy on intestinal mucosal immunity and gut microbiota.					
32122493	8	15	from	effects	1224:1230	arg1	microbiota					1277:1286	gut microbiota	1273:1286	gut microbiota	1273:1286	These findings suggested the potential of CSP as a prebiotics to reduce side effects of Cy on intestinal mucosal immunity and gut microbiota.					
32122493	5	16	theme	rRNA	764:767	arg1	sequencing					769:778	16S rRNA sequencing	760:778	16S rRNA sequencing of fecal samples	760:795	Furthermore, 16S rRNA sequencing of fecal samples was performed.					
32122493	3	17	dep	proteins	599:606	arg1	p-IκB-α					609:615	p-IκB-α	609:615	p-IκB-α	609:615	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	3	17	dep	proteins	599:606	arg1	p65					624:626	NF-κB p65	618:626	NF-κB p65	618:626	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	1	18	from	dysbiosis	332:340	arg1	mice					345:348	mice	345:348	mice	345:348	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	1	19	theme	cultured	198:205	arg1	CSP					243:245	CSP	243:245	CSP	243:245	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	1	19	theme	cultured	198:205	arg1	polysaccharides					226:240	cultured Cordyceps sinensis polysaccharides	198:240	cultured Cordyceps sinensis polysaccharides (CSP)	198:246	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	0	20	theme	Cordyceps	9:17	arg1	polysaccharides					28:42	Cultured Cordyceps sinensis polysaccharides	0:42	Cultured Cordyceps sinensis polysaccharides	0:42	Cultured Cordyceps sinensis polysaccharides modulate intestinal mucosal immunity and gut microbiota in cyclophosphamide-treated mice.					
32122493	1	21	from	immunosuppression	300:316	arg1	mice					345:348	mice	345:348	mice	345:348	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	1	22	theme	Cordyceps	207:215	arg1	CSP					243:245	CSP	243:245	CSP	243:245	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	1	22	theme	Cordyceps	207:215	arg1	polysaccharides					226:240	cultured Cordyceps sinensis polysaccharides	198:240	cultured Cordyceps sinensis polysaccharides (CSP)	198:246	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	0	23	theme	Cultured	0:7	arg1	polysaccharides					28:42	Cultured Cordyceps sinensis polysaccharides	0:42	Cultured Cordyceps sinensis polysaccharides	0:42	Cultured Cordyceps sinensis polysaccharides modulate intestinal mucosal immunity and gut microbiota in cyclophosphamide-treated mice.					
32122493	3	24	theme	CSP	657:659	arg1	administration					661:674	CSP administration	657:674	CSP administration	657:674	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	7	25	theme	probiotics	1029:1038	arg1	abundance					1016:1024	the abundance	1012:1024	the abundance of probiotics (Lactobacillus, Bifidobacterium, Bacteroides)	1012:1084	Taxonomic composition analysis found that CSP increased the abundance of probiotics (Lactobacillus, Bifidobacterium, Bacteroides) and decreased pathogenic bacteria (Clostridium, Flexispira).					
32122493	1	26	theme	sinensis	217:224	arg1	CSP					243:245	CSP	243:245	CSP	243:245	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	1	26	theme	sinensis	217:224	arg1	polysaccharides					226:240	cultured Cordyceps sinensis polysaccharides	198:240	cultured Cordyceps sinensis polysaccharides (CSP)	198:246	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	1	27	theme	polysaccharides	226:240	arg1	effect					188:193	the protective effect	173:193	the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice	173:348	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	0	28	theme	sinensis	19:26	arg1	polysaccharides					28:42	Cultured Cordyceps sinensis polysaccharides	0:42	Cultured Cordyceps sinensis polysaccharides	0:42	Cultured Cordyceps sinensis polysaccharides modulate intestinal mucosal immunity and gut microbiota in cyclophosphamide-treated mice.					
32122493	1	29	theme	microbial	322:330	arg1	dysbiosis					332:340	microbial dysbiosis	322:340	microbial dysbiosis	322:340	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	5	30	theme	samples	789:795	arg1	sequencing					769:778	16S rRNA sequencing	760:778	16S rRNA sequencing of fecal samples	760:795	Furthermore, 16S rRNA sequencing of fecal samples was performed.					
32122493	0	31	theme	intestinal	53:62	arg1	immunity					72:79	intestinal mucosal immunity	53:79	intestinal mucosal immunity	53:79	Cultured Cordyceps sinensis polysaccharides modulate intestinal mucosal immunity and gut microbiota in cyclophosphamide-treated mice.					
32122493	6	32	theme	community	881:889	arg1	diversity					891:899	CSP improved microbial community diversity	858:899	CSP improved microbial community diversity	858:899	α-diversity and β-diversity analysis revealed CSP improved microbial community diversity and modulated the overall structure of gut microbiota.					
32122493	3	33	dep	TLRs	550:553	arg1	TLR-2					556:560	TLR-2	556:560	TLR-2	556:560	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	3	33	dep	TLRs	550:553	arg1	TLR-6					570:574	TLR-6	570:574	TLR-6	570:574	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	3	33	dep	TLRs	550:553	arg1	TLR-4					563:567	TLR-4	563:567	TLR-4	563:567	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	8	34	theme	side	1219:1222	arg1	effects					1224:1230	side effects	1219:1230	side effects of Cy on intestinal mucosal immunity and gut microbiota	1219:1286	These findings suggested the potential of CSP as a prebiotics to reduce side effects of Cy on intestinal mucosal immunity and gut microbiota.					
32122493	7	35	theme	pathogenic	1100:1109	arg1	Clostridium					1121:1131	Clostridium	1121:1131	Clostridium	1121:1131	Taxonomic composition analysis found that CSP increased the abundance of probiotics (Lactobacillus, Bifidobacterium, Bacteroides) and decreased pathogenic bacteria (Clostridium, Flexispira).					
32122493	7	35	theme	pathogenic	1100:1109	arg1	bacteria					1111:1118	pathogenic bacteria	1100:1118	pathogenic bacteria (Clostridium, Flexispira)	1100:1144	Taxonomic composition analysis found that CSP increased the abundance of probiotics (Lactobacillus, Bifidobacterium, Bacteroides) and decreased pathogenic bacteria (Clostridium, Flexispira).					
32122493	6	36	theme	microbial	871:879	arg1	community					881:889	CSP improved microbial community	858:889	CSP improved microbial community diversity	858:899	α-diversity and β-diversity analysis revealed CSP improved microbial community diversity and modulated the overall structure of gut microbiota.					
32122493	7	37	dep	probiotics	1029:1038	arg1	Bifidobacterium					1056:1070	Bifidobacterium	1056:1070	Bifidobacterium	1056:1070	Taxonomic composition analysis found that CSP increased the abundance of probiotics (Lactobacillus, Bifidobacterium, Bacteroides) and decreased pathogenic bacteria (Clostridium, Flexispira).					
32122493	7	37	dep	probiotics	1029:1038	arg1	Lactobacillus					1041:1053	Lactobacillus	1041:1053	Lactobacillus	1041:1053	Taxonomic composition analysis found that CSP increased the abundance of probiotics (Lactobacillus, Bifidobacterium, Bacteroides) and decreased pathogenic bacteria (Clostridium, Flexispira).					
32122493	7	37	dep	probiotics	1029:1038	arg1	Bacteroides					1073:1083	Bacteroides	1073:1083	Bacteroides	1073:1083	Taxonomic composition analysis found that CSP increased the abundance of probiotics (Lactobacillus, Bifidobacterium, Bacteroides) and decreased pathogenic bacteria (Clostridium, Flexispira).					
32122493	8	38	theme	intestinal	1241:1250	arg1	immunity					1260:1267	intestinal mucosal immunity	1241:1267	intestinal mucosal immunity	1241:1267	These findings suggested the potential of CSP as a prebiotics to reduce side effects of Cy on intestinal mucosal immunity and gut microbiota.					
32122493	5	39	theme	16S	760:762	arg1	sequencing					769:778	16S rRNA sequencing	760:778	16S rRNA sequencing of fecal samples	760:795	Furthermore, 16S rRNA sequencing of fecal samples was performed.					
32122493	6	40	theme	improved	862:869	arg1	community					881:889	CSP improved microbial community	858:889	CSP improved microbial community diversity	858:899	α-diversity and β-diversity analysis revealed CSP improved microbial community diversity and modulated the overall structure of gut microbiota.					
32122493	7	41	theme	composition	966:976	arg1	analysis					978:985	Taxonomic composition analysis	956:985	Taxonomic composition analysis	956:985	Taxonomic composition analysis found that CSP increased the abundance of probiotics (Lactobacillus, Bifidobacterium, Bacteroides) and decreased pathogenic bacteria (Clostridium, Flexispira).					
32122493	1	42	theme	present	138:144	arg1	study					146:150	The present study	134:150	The present study	134:150	The present study aimed to investigate the protective effect of cultured Cordyceps sinensis polysaccharides (CSP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice.					
32122493	0	43	theme	mucosal	64:70	arg1	immunity					72:79	intestinal mucosal immunity	53:79	intestinal mucosal immunity	53:79	Cultured Cordyceps sinensis polysaccharides modulate intestinal mucosal immunity and gut microbiota in cyclophosphamide-treated mice.					
32122493	6	44	theme	CSP	858:860	arg1	community					881:889	CSP improved microbial community	858:889	CSP improved microbial community diversity	858:899	α-diversity and β-diversity analysis revealed CSP improved microbial community diversity and modulated the overall structure of gut microbiota.					
32122493	3	45	theme	key	595:597	arg1	proteins					599:606	NF-κB pathway key proteins	581:606	NF-κB pathway key proteins (p-IκB-α, NF-κB p65)	581:627	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	4	46	theme	Cy	733:734	arg1	treatment					736:744	Cy treatment	733:744	Cy treatment	733:744	Moreover, CSP recovered SCFAs levels which decreased by Cy treatment.					
32122493	2	47	dep	IL-21	475:479	arg1	IL-4					421:424	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	IL-4	421:424	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	47	dep	IL-21	475:479	arg1	IL-17					440:444	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	IL-17	440:444	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	47	dep	IL-21	475:479	arg1	IL-10					447:451	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	IL-10	447:451	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	47	dep	IL-21	475:479	arg1	IL-2					469:472	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	IL-2	469:472	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	47	dep	IL-21	475:479	arg1	IL-13					427:431	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	IL-13	427:431	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	47	dep	IL-21	475:479	arg1	IL-6					434:437	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	IL-6	434:437	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	47	dep	IL-21	475:479	arg1	TGF-β3					454:459	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	TGF-β3	454:459	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	47	dep	IL-21	475:479	arg1	IFN-γ					414:418	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	IFN-γ	414:418	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	2	47	dep	IL-21	475:479	arg1	TNF-α					462:466	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	TNF-α	462:466	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	4	48	theme	SCFAs	701:705	arg1	levels					707:712	SCFAs levels	701:712	SCFAs levels which decreased by Cy treatment	701:744	Moreover, CSP recovered SCFAs levels which decreased by Cy treatment.					
32122493	5	49	theme	fecal	783:787	arg1	samples					789:795	fecal samples	783:795	fecal samples	783:795	Furthermore, 16S rRNA sequencing of fecal samples was performed.					
32122493	3	50	theme	NF-κB	581:585	arg1	proteins					599:606	NF-κB pathway key proteins	581:606	NF-κB pathway key proteins (p-IκB-α, NF-κB p65)	581:627	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	8	51	theme	mucosal	1252:1258	arg1	immunity					1260:1267	intestinal mucosal immunity	1241:1267	intestinal mucosal immunity	1241:1267	These findings suggested the potential of CSP as a prebiotics to reduce side effects of Cy on intestinal mucosal immunity and gut microbiota.					
32122493	8	52	theme	Cy	1235:1236	arg1	effects					1224:1230	side effects	1219:1230	side effects of Cy on intestinal mucosal immunity and gut microbiota	1219:1286	These findings suggested the potential of CSP as a prebiotics to reduce side effects of Cy on intestinal mucosal immunity and gut microbiota.					
32122493	0	53	theme	gut	85:87	arg1	microbiota					89:98	gut microbiota	85:98	gut microbiota	85:98	Cultured Cordyceps sinensis polysaccharides modulate intestinal mucosal immunity and gut microbiota in cyclophosphamide-treated mice.					
32122493	3	54	theme	pathway	587:593	arg1	proteins					599:606	NF-κB pathway key proteins	581:606	NF-κB pathway key proteins (p-IκB-α, NF-κB p65)	581:627	TLRs (TLR-2, TLR-4, TLR-6) and NF-κB pathway key proteins (p-IκB-α, NF-κB p65) were also upregulated after CSP administration.					
32122493	2	55	theme	IL-12	407:411	arg1	IL-21					475:479	IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21)	407:480	IL-21	475:479	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
32122493	6	56	theme	microbiota	944:953	arg1	structure					927:935	the overall structure	915:935	the overall structure of gut microbiota	915:953	α-diversity and β-diversity analysis revealed CSP improved microbial community diversity and modulated the overall structure of gut microbiota.					
32122493	6	57	theme	overall	919:925	arg1	structure					927:935	the overall structure	915:935	the overall structure of gut microbiota	915:953	α-diversity and β-diversity analysis revealed CSP improved microbial community diversity and modulated the overall structure of gut microbiota.					
32122493	6	58	theme	gut	940:942	arg1	microbiota					944:953	gut microbiota	940:953	gut microbiota	940:953	α-diversity and β-diversity analysis revealed CSP improved microbial community diversity and modulated the overall structure of gut microbiota.					
32122493	8	59	theme	CSP	1189:1191	arg1	potential					1176:1184	the potential	1172:1184	the potential of CSP as a prebiotics to reduce side effects of Cy on intestinal mucosal immunity and gut microbiota	1172:1286	These findings suggested the potential of CSP as a prebiotics to reduce side effects of Cy on intestinal mucosal immunity and gut microbiota.					
32122493	2	60	theme	transcription	486:498	arg1	factors					500:506	transcription factors	486:506	transcription factors production	486:517	Results showed that CSP stimulated cytokines secretion (IL-12, IFN-γ, IL-4, IL-13, IL-6, IL-17, IL-10, TGF-β3, TNF-α, IL-2, IL-21) and transcription factors production (T-bet, GATA-3, RORγt, Foxp3).					
35049867	6	0	theme	degradation	958:968	arg1	GLP-HV					980:985	GLP-HV	980:985	GLP-HV	980:985	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	6	0	theme	degradation	958:968	arg1	products					970:977	its degradation products	954:977	its degradation products (GLP-HV, GLP-H and GLP-V)	954:1003	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	6	0	theme	degradation	958:968	arg1	GLP-V					998:1002	GLP-V	998:1002	GLP-V	998:1002	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	6	0	theme	degradation	958:968	arg1	GLP-H					988:992	GLP-H	988:992	GLP-H	988:992	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	2	1	dep	peroxide	300:307	arg1	optimized					347:355	optimized	347:355	optimized by Box-Behnken Design (BBD)	347:383	After its degradation by hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box-Behnken Design (BBD), a new product of GLP-HV will be generated.					
35049867	2	1	dep	peroxide	300:307	arg1	combined					309:316	combined	309:316	combined with vitamin C (H2O2-Vc)	309:341	After its degradation by hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box-Behnken Design (BBD), a new product of GLP-HV will be generated.					
35049867	6	2	theme	total	923:927	arg1	content					935:941	The total sugar content	919:941	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V)	919:1003	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	6	2	theme	total	923:927	arg1	%					1022:1022	more than 97%	1010:1022	more than 97%	1010:1022	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	9	3	contain	had	1344:1346	arg2	effect					1368:1373	the best inhibition effect	1348:1373	the best inhibition effect on α-glucosidase	1348:1390	GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive.					
35049867	9	3	contain	had	1344:1346	arg1	GLP-HV					1337:1342	GLP-HV	1337:1342	GLP-HV	1337:1342	GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive.					
35049867	10	4	contain	had	1484:1486	arg2	quenching					1570:1578	dynamic quenching	1562:1578	dynamic quenching	1562:1578	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	10	4	contain	had	1484:1486	arg1	It					1481:1482	It	1481:1482	It	1481:1482	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	10	4	contain	had	1484:1486	arg2	effect					1508:1513	a certain quenching effect	1488:1513	a certain quenching effect	1488:1513	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	9	5	from	effect	1368:1373	arg1	α-glucosidase					1378:1390	α-glucosidase	1378:1390	α-glucosidase	1378:1390	GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive.					
35049867	4	6	theme	UV	645:646	arg1	spectrum					648:655	UV spectrum	645:655	UV spectrum	645:655	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	6	theme	UV	645:646	arg1	characteristics					561:575	chemical characteristics	552:575	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro)	537:718	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	9	7	theme	inhibition	1357:1366	arg1	effect					1368:1373	the best inhibition effect	1348:1373	the best inhibition effect on α-glucosidase	1348:1390	GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive.					
35049867	5	8	theme	reaction	887:894	arg1	temperature					896:906	reaction temperature	887:906	reaction temperature	887:906	The results showed that the optimal conditions for H2O2-Vc degradation were as follows: H2O2-Vc concentration was 18.7 mM, reaction time was 0.5 h, and reaction temperature was 56 °C.					
35049867	8	9	from	effect	1234:1239	arg1	α-glucosidase					1244:1256	α-glucosidase	1244:1256	α-glucosidase	1244:1256	Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively.					
35049867	7	10	theme	SEM	1089:1091	arg1	analysis					1093:1100	The SEM analysis	1085:1100	The SEM analysis	1085:1100	The SEM analysis demonstrated that H2O2-Vc made the structure loose and broken.					
35049867	2	11	theme	hydrogen	291:298	arg1	peroxide					300:307	hydrogen peroxide	291:307	hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box-Behnken Design (BBD)	291:383	After its degradation by hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box-Behnken Design (BBD), a new product of GLP-HV will be generated.					
35049867	3	12	theme	GLP-V	498:502	arg1	products					461:468	two products	457:468	two products of GLP-H (H2O2-treated) and GLP-V (Vc-treated)	457:515	While using GLP as control, two products of GLP-H (H2O2-treated) and GLP-V (Vc-treated) were also produced.					
35049867	5	13	theme	H2O2-Vc	786:792	arg1	degradation					794:804	H2O2-Vc degradation	786:804	H2O2-Vc degradation	786:804	The results showed that the optimal conditions for H2O2-Vc degradation were as follows: H2O2-Vc concentration was 18.7 mM, reaction time was 0.5 h, and reaction temperature was 56 °C.					
35049867	6	14	dep	products	970:977	arg1	GLP-HV					980:985	GLP-HV	980:985	GLP-HV	980:985	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	6	14	dep	products	970:977	arg1	products					970:977	its degradation products	954:977	its degradation products (GLP-HV, GLP-H and GLP-V)	954:1003	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	6	14	dep	products	970:977	arg1	GLP-V					998:1002	GLP-V	998:1002	GLP-V	998:1002	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	6	14	dep	products	970:977	arg1	GLP-H					988:992	GLP-H	988:992	GLP-H	988:992	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	1	15	theme	good	190:193	arg1	activities					209:218	good physiological activities	190:218	good physiological activities	190:218	Gracilaria lemaneiformis polysaccharide (GLP) exhibits good physiological activities, and it is more beneficial as it is degraded.					
35049867	8	16	contain	had	1205:1207	arg1	GLP-H					1188:1192	GLP-H	1188:1192	GLP-H	1188:1192	Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively.					
35049867	8	16	contain	had	1205:1207	arg1	GLP-V					1199:1203	GLP-V	1199:1203	GLP-V	1199:1203	Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively.					
35049867	8	16	contain	had	1205:1207	arg1	GLP-HV					1180:1185	GLP-HV	1180:1185	GLP-HV	1180:1185	Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively.					
35049867	8	16	contain	had	1205:1207	arg1	GLP					1175:1177	GLP	1175:1177	GLP	1175:1177	Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively.					
35049867	8	16	contain	had	1205:1207	arg2	effect					1234:1239	inhibition effect	1223:1239	inhibition effect on α-glucosidase	1223:1256	Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively.					
35049867	9	17	theme	best	1352:1355	arg1	effect					1368:1373	the best inhibition effect	1348:1373	the best inhibition effect on α-glucosidase	1348:1390	GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive.					
35049867	9	18	theme	dose-dependent	1397:1410	arg1	manner					1412:1417	a dose-dependent manner	1395:1417	a dose-dependent manner	1395:1417	GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive.					
35049867	1	19	theme	physiological	195:207	arg1	activities					209:218	good physiological activities	190:218	good physiological activities	190:218	Gracilaria lemaneiformis polysaccharide (GLP) exhibits good physiological activities, and it is more beneficial as it is degraded.					
35049867	4	20	theme	hypolipidemic	687:699	arg1	activity					701:708	hypolipidemic activity	687:708	hypolipidemic activity	687:708	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	20	theme	hypolipidemic	687:699	arg1	characteristics					561:575	chemical characteristics	552:575	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro)	537:718	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	21	theme	monosaccharide	617:630	arg1	composition					632:642	monosaccharide composition	617:642	monosaccharide composition	617:642	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	21	theme	monosaccharide	617:630	arg1	characteristics					561:575	chemical characteristics	552:575	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro)	537:718	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	2	22	theme	vitamin	323:329	arg1	H2O2-Vc					334:340	H2O2-Vc	334:340	H2O2-Vc	334:340	After its degradation by hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box-Behnken Design (BBD), a new product of GLP-HV will be generated.					
35049867	2	22	theme	vitamin	323:329	arg1	C					331:331	vitamin C	323:331	vitamin C (H2O2-Vc)	323:341	After its degradation by hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box-Behnken Design (BBD), a new product of GLP-HV will be generated.					
35049867	5	23	theme	H2O2-Vc	823:829	arg1	concentration					831:843	H2O2-Vc concentration	823:843	H2O2-Vc concentration	823:843	The results showed that the optimal conditions for H2O2-Vc degradation were as follows: H2O2-Vc concentration was 18.7 mM, reaction time was 0.5 h, and reaction temperature was 56 °C.					
35049867	5	24	theme	optimal	763:769	arg1	conditions					771:780	the optimal conditions	759:780	the optimal conditions for H2O2-Vc degradation	759:804	The results showed that the optimal conditions for H2O2-Vc degradation were as follows: H2O2-Vc concentration was 18.7 mM, reaction time was 0.5 h, and reaction temperature was 56 °C.					
35049867	8	25	theme	IC50	1269:1272	arg1	value					1274:1278	their IC50 value	1263:1278	their IC50 value	1263:1278	Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively.					
35049867	3	26	dep	GLP-H	473:477	arg1	H2O2-treated					480:491	H2O2-treated	480:491	H2O2-treated	480:491	While using GLP as control, two products of GLP-H (H2O2-treated) and GLP-V (Vc-treated) were also produced.					
35049867	4	27	theme	sugar	584:588	arg1	content					590:596	total sugar content	578:596	total sugar content	578:596	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	27	theme	sugar	584:588	arg1	characteristics					561:575	chemical characteristics	552:575	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro)	537:718	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	2	28	theme	GLP-HV	403:408	arg1	product					392:398	a new product	386:398	a new product of GLP-HV	386:408	After its degradation by hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box-Behnken Design (BBD), a new product of GLP-HV will be generated.					
35049867	8	29	theme	inhibition	1223:1232	arg1	effect					1234:1239	inhibition effect	1223:1239	inhibition effect on α-glucosidase	1223:1256	Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively.					
35049867	4	30	theme	total	578:582	arg1	content					590:596	total sugar content	578:596	total sugar content	578:596	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	30	theme	total	578:582	arg1	characteristics					561:575	chemical characteristics	552:575	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro)	537:718	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	0	31	theme	α-glucosidase	40:52	arg1	Activity					54:61	α-glucosidase Activity	40:61	α-glucosidase Activity	40:61	Optimized Degradation and Inhibition of α-glucosidase Activity by Gracilaria lemaneiformis Polysaccharide and Its Production In Vitro.					
35049867	5	32	theme	reaction	858:865	arg1	time					867:870	reaction time	858:870	reaction time	858:870	The results showed that the optimal conditions for H2O2-Vc degradation were as follows: H2O2-Vc concentration was 18.7 mM, reaction time was 0.5 h, and reaction temperature was 56 °C.					
35049867	7	33	theme	structure	1137:1145	arg1	loose					1147:1151	the structure loose	1133:1151	the structure loose	1133:1151	The SEM analysis demonstrated that H2O2-Vc made the structure loose and broken.					
35049867	2	34	theme	new	388:390	arg1	product					392:398	a new product	386:398	a new product of GLP-HV	386:408	After its degradation by hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box-Behnken Design (BBD), a new product of GLP-HV will be generated.					
35049867	6	35	theme	GLP	946:948	arg1	content					935:941	The total sugar content	919:941	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V)	919:1003	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	6	35	theme	GLP	946:948	arg1	%					1022:1022	more than 97%	1010:1022	more than 97%	1010:1022	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	9	36	theme	mixed	1434:1438	arg1	type					1440:1443	the mixed type	1430:1443	the mixed type of competitive and non-competitive	1430:1478	GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive.					
35049867	9	36	theme	mixed	1434:1438	arg1	which					1420:1424	which	1420:1424	which	1420:1424	GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive.					
35049867	10	37	theme	quenching	1498:1506	arg1	effect					1508:1513	a certain quenching effect	1488:1513	a certain quenching effect	1488:1513	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	10	37	theme	quenching	1498:1506	arg1	quenching					1570:1578	dynamic quenching	1562:1578	dynamic quenching	1562:1578	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	10	38	theme	dynamic	1562:1568	arg1	effect					1508:1513	a certain quenching effect	1488:1513	a certain quenching effect	1488:1513	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	10	38	theme	dynamic	1562:1568	arg1	quenching					1570:1578	dynamic quenching	1562:1578	dynamic quenching	1562:1578	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	4	39	theme	morphological	658:670	arg1	characteristics					561:575	chemical characteristics	552:575	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro)	537:718	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	39	theme	morphological	658:670	arg1	structure					672:680	morphological structure	658:680	morphological structure	658:680	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	0	40	theme	Activity	54:61	arg1	Inhibition					26:35	Inhibition	26:35	Inhibition of α-glucosidase Activity by Gracilaria lemaneiformis Polysaccharide and Its Production In Vitro	26:132	Optimized Degradation and Inhibition of α-glucosidase Activity by Gracilaria lemaneiformis Polysaccharide and Its Production In Vitro.					
35049867	0	40	theme	Activity	54:61	arg1	Degradation					10:20	Degradation	10:20	Degradation	10:20	Optimized Degradation and Inhibition of α-glucosidase Activity by Gracilaria lemaneiformis Polysaccharide and Its Production In Vitro.					
35049867	8	41	theme	1.923	1310:1314	arg1	mg/mL					1316:1320	1.923 mg/mL	1310:1320	1.923 mg/mL	1310:1320	Moreover, GLP, GLP-HV, GLP-H, and GLP-V had significantly inhibition effect on α-glucosidase, and their IC50 value were 3.957, 0.265, 1.651, and 1.923 mg/mL, respectively.					
35049867	1	42	theme	Gracilaria	135:144	arg1	GLP					176:178	GLP	176:178	GLP	176:178	Gracilaria lemaneiformis polysaccharide (GLP) exhibits good physiological activities, and it is more beneficial as it is degraded.					
35049867	1	42	theme	Gracilaria	135:144	arg1	polysaccharide					160:173	Gracilaria lemaneiformis polysaccharide	135:173	Gracilaria lemaneiformis polysaccharide (GLP)	135:179	Gracilaria lemaneiformis polysaccharide (GLP) exhibits good physiological activities, and it is more beneficial as it is degraded.					
35049867	4	43	dep	characteristics	561:575	arg1	structure					672:680	morphological structure	658:680	morphological structure	658:680	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	43	dep	characteristics	561:575	arg1	content					590:596	total sugar content	578:596	total sugar content	578:596	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	43	dep	characteristics	561:575	arg1	composition					632:642	monosaccharide composition	617:642	monosaccharide composition	617:642	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	43	dep	characteristics	561:575	arg1	spectrum					648:655	UV spectrum	645:655	UV spectrum	645:655	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	43	dep	characteristics	561:575	arg1	characteristics					561:575	chemical characteristics	552:575	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro)	537:718	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	43	dep	characteristics	561:575	arg1	activity					701:708	hypolipidemic activity	687:708	hypolipidemic activity	687:708	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	43	dep	characteristics	561:575	arg1	weight					609:614	molecular weight	599:614	molecular weight	599:614	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	1	44	theme	lemaneiformis	146:158	arg1	GLP					176:178	GLP	176:178	GLP	176:178	Gracilaria lemaneiformis polysaccharide (GLP) exhibits good physiological activities, and it is more beneficial as it is degraded.					
35049867	1	44	theme	lemaneiformis	146:158	arg1	polysaccharide					160:173	Gracilaria lemaneiformis polysaccharide	135:173	Gracilaria lemaneiformis polysaccharide (GLP)	135:179	Gracilaria lemaneiformis polysaccharide (GLP) exhibits good physiological activities, and it is more beneficial as it is degraded.					
35049867	4	45	theme	chemical	552:559	arg1	structure					672:680	morphological structure	658:680	morphological structure	658:680	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	45	theme	chemical	552:559	arg1	content					590:596	total sugar content	578:596	total sugar content	578:596	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	45	theme	chemical	552:559	arg1	composition					632:642	monosaccharide composition	617:642	monosaccharide composition	617:642	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	45	theme	chemical	552:559	arg1	spectrum					648:655	UV spectrum	645:655	UV spectrum	645:655	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	45	theme	chemical	552:559	arg1	characteristics					561:575	chemical characteristics	552:575	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro)	537:718	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	45	theme	chemical	552:559	arg1	activity					701:708	hypolipidemic activity	687:708	hypolipidemic activity	687:708	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	45	theme	chemical	552:559	arg1	weight					609:614	molecular weight	599:614	molecular weight	599:614	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	9	46	dep	had	1344:1346	arg1	type					1440:1443	the mixed type	1430:1443	the mixed type of competitive and non-competitive	1430:1478	GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive.					
35049867	9	46	dep	had	1344:1346	arg1	which					1420:1424	which	1420:1424	which	1420:1424	GLP-HV had the best inhibition effect on α-glucosidase in a dose-dependent manner, which was the mixed type of competitive and non-competitive.					
35049867	10	47	from	effect	1508:1513	arg1	fluorescence					1518:1529	fluorescence	1518:1529	fluorescence of α-glucosidase	1518:1546	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	3	48	dep	GLP-V	498:502	arg1	Vc-treated					505:514	Vc-treated	505:514	Vc-treated	505:514	While using GLP as control, two products of GLP-H (H2O2-treated) and GLP-V (Vc-treated) were also produced.					
35049867	10	49	theme	α-glucosidase	1534:1546	arg1	fluorescence					1518:1529	fluorescence	1518:1529	fluorescence of α-glucosidase	1518:1546	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	0	50	theme	lemaneiformis	77:89	arg1	Polysaccharide					91:104	Gracilaria lemaneiformis Polysaccharide	66:104	Gracilaria lemaneiformis Polysaccharide	66:104	Optimized Degradation and Inhibition of α-glucosidase Activity by Gracilaria lemaneiformis Polysaccharide and Its Production In Vitro.					
35049867	4	51	dep	products	543:550	arg1	structure					672:680	morphological structure	658:680	morphological structure	658:680	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	51	dep	products	543:550	arg1	content					590:596	total sugar content	578:596	total sugar content	578:596	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	51	dep	products	543:550	arg1	composition					632:642	monosaccharide composition	617:642	monosaccharide composition	617:642	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	51	dep	products	543:550	arg1	spectrum					648:655	UV spectrum	645:655	UV spectrum	645:655	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	51	dep	products	543:550	arg1	characteristics					561:575	chemical characteristics	552:575	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro)	537:718	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	51	dep	products	543:550	arg1	activity					701:708	hypolipidemic activity	687:708	hypolipidemic activity	687:708	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	51	dep	products	543:550	arg1	weight					609:614	molecular weight	599:614	molecular weight	599:614	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	6	52	theme	products	970:977	arg1	content					935:941	The total sugar content	919:941	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V)	919:1003	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	6	52	theme	products	970:977	arg1	%					1022:1022	more than 97%	1010:1022	more than 97%	1010:1022	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	3	53	theme	GLP-H	473:477	arg1	products					461:468	two products	457:468	two products of GLP-H (H2O2-treated) and GLP-V (Vc-treated)	457:515	While using GLP as control, two products of GLP-H (H2O2-treated) and GLP-V (Vc-treated) were also produced.					
35049867	4	54	theme	molecular	599:607	arg1	weight					609:614	molecular weight	599:614	molecular weight	599:614	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	4	54	theme	molecular	599:607	arg1	characteristics					561:575	chemical characteristics	552:575	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro)	537:718	These products chemical characteristics (total sugar content, molecular weight, monosaccharide composition, UV spectrum, morphological structure, and hypolipidemic activity in vitro) were assessed.					
35049867	10	55	theme	certain	1490:1496	arg1	effect					1508:1513	a certain quenching effect	1488:1513	a certain quenching effect	1488:1513	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	10	55	theme	certain	1490:1496	arg1	quenching					1570:1578	dynamic quenching	1562:1578	dynamic quenching	1562:1578	It had a certain quenching effect on fluorescence of α-glucosidase, which may be dynamic quenching.					
35049867	0	56	theme	Gracilaria	66:75	arg1	Polysaccharide					91:104	Gracilaria lemaneiformis Polysaccharide	66:104	Gracilaria lemaneiformis Polysaccharide	66:104	Optimized Degradation and Inhibition of α-glucosidase Activity by Gracilaria lemaneiformis Polysaccharide and Its Production In Vitro.					
35049867	2	57	theme	Box-Behnken	360:370	arg1	BBD					380:382	BBD	380:382	BBD	380:382	After its degradation by hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box-Behnken Design (BBD), a new product of GLP-HV will be generated.					
35049867	2	57	theme	Box-Behnken	360:370	arg1	Design					372:377	Box-Behnken Design	360:377	Box-Behnken Design (BBD)	360:383	After its degradation by hydrogen peroxide combined with vitamin C (H2O2-Vc) and optimized by Box-Behnken Design (BBD), a new product of GLP-HV will be generated.					
35049867	6	58	theme	sugar	929:933	arg1	content					935:941	The total sugar content	919:941	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V)	919:1003	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
35049867	6	58	theme	sugar	929:933	arg1	%					1022:1022	more than 97%	1010:1022	more than 97%	1010:1022	The total sugar content of GLP and its degradation products (GLP-HV, GLP-H and GLP-V) were more than 97%, and their monosaccharides are mainly glucose and galactose.					
33319171	5	0	theme	unexplored	801:810	arg1	details					812:818	hitherto unexplored details	792:818	hitherto unexplored details of the native shield architecture	792:852	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	6	1	theme	shielding	953:961	arg1	effect					963:968	the shielding effect	949:968	the shielding effect	949:968	We have also derived a measure to quantify the shielding effect over the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design.					
33319171	5	2	theme	integrated	694:703	arg1	approach					705:712	This integrated approach	689:712	This integrated approach including quantitative comparison with cryo-electron microscopy data	689:781	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	6	3	theme	relative	1029:1036	arg1	vulnerability					1038:1050	relative vulnerability	1029:1050	relative vulnerability	1029:1050	We have also derived a measure to quantify the shielding effect over the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design.					
33319171	4	4	theme	first	507:511	arg1	model					531:535	the first complete all-atom model	503:535	the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans	503:620	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	4	4	theme	first	507:511	arg1	This					495:498	This	495:498	This	495:498	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	1	5	theme	Env	153:155	arg1	protein					158:164	the HIV-1 envelope (Env) protein	133:164	the HIV-1 envelope (Env) protein	133:164	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	4	6	theme	shield	563:568	arg1	Env					546:548	HIV-1 Env	540:548	HIV-1 Env SOSIP glycan shield	540:568	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	2	7	theme	glycans	269:275	arg1	flexibility					254:264	flexibility	254:264	flexibility	254:264	However, the molecular complexity and flexibility of glycans make experimental studies a challenge.					
33319171	2	7	theme	glycans	269:275	arg1	complexity					239:248	the molecular complexity	225:248	the molecular complexity	225:248	However, the molecular complexity and flexibility of glycans make experimental studies a challenge.					
33319171	3	8	theme	glycosylated	384:395	arg1	Env					403:405	fully glycosylated HIV-1 Env	378:405	fully glycosylated HIV-1 Env with graph theory	378:423	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33319171	0	9	theme	Atomistic	84:92	arg1	Detail					94:99	Atomistic Detail	84:99	Atomistic Detail	84:99	Quantification of the Resilience and Vulnerability of HIV-1 Native Glycan Shield at Atomistic Detail.					
33319171	3	10	gly	glycosylated	384:395	arg1	Env					403:405	fully glycosylated HIV-1 Env	378:405	fully glycosylated HIV-1 Env with graph theory	378:423	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33319171	6	11	theme	resilience	1056:1065	arg1	regions					1018:1024	regions	1018:1024	regions of relative vulnerability and resilience of the shield	1018:1079	We have also derived a measure to quantify the shielding effect over the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design.					
33319171	5	12	theme	microscopy	767:776	arg1	data					778:781	cryo-electron microscopy data	753:781	cryo-electron microscopy data	753:781	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	3	13	theme	topology	485:492	arg1	features					462:469	immunologically important features	436:469	immunologically important features of the shield topology	436:492	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33319171	3	14	with	Env	403:405	arg1	theory					418:423	graph theory	412:423	graph theory	412:423	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33319171	4	15	theme	glycan	674:679	arg1	insights					658:665	physiologically relevant insights	633:665	physiologically relevant insights of the glycan shield	633:686	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	5	16	theme	shield	834:839	arg1	architecture					841:852	the native shield architecture	823:852	the native shield architecture	823:852	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	3	17	theme	Env	403:405	arg1	modeling					366:373	high-throughput atomistic modeling	340:373	high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory	340:423	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33319171	4	18	theme	oligomannose	589:600	arg1	glycans					614:620	both oligomannose and complex glycans	584:620	glycans	614:620	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	6	19	theme	immunogen	1115:1123	arg1	design					1125:1130	rational immunogen design	1106:1130	rational immunogen design	1106:1130	We have also derived a measure to quantify the shielding effect over the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design.					
33319171	5	20	theme	architecture	841:852	arg1	difference					862:871	its difference	858:871	its difference from the high-mannose glycoform	858:903	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	5	20	theme	architecture	841:852	arg1	details					812:818	hitherto unexplored details	792:818	hitherto unexplored details of the native shield architecture	792:852	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	6	21	theme	rational	1106:1113	arg1	design					1125:1130	rational immunogen design	1106:1130	rational immunogen design	1106:1130	We have also derived a measure to quantify the shielding effect over the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design.					
33319171	3	22	theme	graph	412:416	arg1	theory					418:423	graph theory	412:423	graph theory	412:423	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33319171	4	23	theme	Env	546:548	arg1	model					531:535	the first complete all-atom model	503:535	the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans	503:620	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	4	23	theme	Env	546:548	arg1	This					495:498	This	495:498	This	495:498	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	2	24	theme	experimental	282:293	arg1	studies					295:301	experimental studies	282:301	experimental studies	282:301	However, the molecular complexity and flexibility of glycans make experimental studies a challenge.					
33319171	0	25	theme	Resilience	22:31	arg1	Quantification					0:13	Quantification	0:13	Quantification of the Resilience and Vulnerability of HIV-1 Native Glycan	0:72	Quantification of the Resilience and Vulnerability of HIV-1 Native Glycan Shield at Atomistic Detail.					
33319171	4	26	theme	HIV-1	540:544	arg1	Env					546:548	HIV-1 Env	540:548	HIV-1 Env SOSIP glycan shield	540:568	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	1	27	gly	glycosylation	116:128	arg1	protein					158:164	the HIV-1 envelope (Env) protein	133:164	the HIV-1 envelope (Env) protein	133:164	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	1	28	from	glycosylation	116:128	arg1	protein					158:164	the HIV-1 envelope (Env) protein	133:164	the HIV-1 envelope (Env) protein	133:164	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	5	29	theme	native	827:832	arg1	architecture					841:852	the native shield architecture	823:852	the native shield architecture	823:852	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	1	30	theme	Dense	102:106	arg1	shield					176:181	shield	176:181	shield	176:181	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	1	30	theme	Dense	102:106	arg1	glycosylation					116:128	Dense surface glycosylation	102:128	Dense surface glycosylation on the HIV-1 envelope (Env) protein	102:164	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	5	31	with	comparison	737:746	arg1	data					778:781	cryo-electron microscopy data	753:781	cryo-electron microscopy data	753:781	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	0	32	theme	Vulnerability	37:49	arg1	Quantification					0:13	Quantification	0:13	Quantification of the Resilience and Vulnerability of HIV-1 Native Glycan	0:72	Quantification of the Resilience and Vulnerability of HIV-1 Native Glycan Shield at Atomistic Detail.					
33319171	5	33	theme	quantitative	724:735	arg1	comparison					737:746	quantitative comparison	724:746	quantitative comparison with cryo-electron microscopy data	724:781	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	5	34	theme	high-mannose	882:893	arg1	glycoform					895:903	the high-mannose glycoform	878:903	the high-mannose glycoform	878:903	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	4	35	theme	relevant	649:656	arg1	insights					658:665	physiologically relevant insights	633:665	physiologically relevant insights of the glycan shield	633:686	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	6	36	theme	shield	1074:1079	arg1	vulnerability					1038:1050	relative vulnerability	1029:1050	relative vulnerability	1029:1050	We have also derived a measure to quantify the shielding effect over the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design.					
33319171	6	36	theme	shield	1074:1079	arg1	resilience					1056:1065	resilience	1056:1065	resilience	1056:1065	We have also derived a measure to quantify the shielding effect over the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design.					
33319171	4	37	theme	all-atom	522:529	arg1	model					531:535	the first complete all-atom model	503:535	the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans	503:620	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	4	37	theme	all-atom	522:529	arg1	This					495:498	This	495:498	This	495:498	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	6	38	theme	vulnerability	1038:1050	arg1	regions					1018:1024	regions	1018:1024	regions of relative vulnerability and resilience of the shield	1018:1079	We have also derived a measure to quantify the shielding effect over the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design.					
33319171	5	39	theme	cryo-electron	753:765	arg1	microscopy					767:776	cryo-electron microscopy	753:776	cryo-electron microscopy data	753:781	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	3	40	theme	atomistic	356:364	arg1	modeling					366:373	high-throughput atomistic modeling	340:373	high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory	340:423	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33319171	3	41	theme	important	452:460	arg1	features					462:469	immunologically important features	436:469	immunologically important features of the shield topology	436:492	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33319171	0	42	theme	HIV-1	54:58	arg1	Glycan					67:72	HIV-1 Native Glycan	54:72	HIV-1 Native Glycan	54:72	Quantification of the Resilience and Vulnerability of HIV-1 Native Glycan Shield at Atomistic Detail.					
33319171	4	43	theme	complete	513:520	arg1	model					531:535	the first complete all-atom model	503:535	the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans	503:620	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	4	43	theme	complete	513:520	arg1	This					495:498	This	495:498	This	495:498	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	1	44	from	system	208:213	arg1	shield					176:181	shield	176:181	shield	176:181	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	1	44	from	system	208:213	arg1	glycosylation					116:128	Dense surface glycosylation	102:128	Dense surface glycosylation on the HIV-1 envelope (Env) protein	102:164	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	5	45	from	glycoform	895:903	arg1	difference					862:871	its difference	858:871	its difference from the high-mannose glycoform	858:903	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	5	45	from	glycoform	895:903	arg1	details					812:818	hitherto unexplored details	792:818	hitherto unexplored details of the native shield architecture	792:852	This integrated approach including quantitative comparison with cryo-electron microscopy data provides hitherto unexplored details of the native shield architecture and its difference from the high-mannose glycoform.					
33319171	1	46	theme	surface	108:114	arg1	shield					176:181	shield	176:181	shield	176:181	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	1	46	theme	surface	108:114	arg1	glycosylation					116:128	Dense surface glycosylation	102:128	Dense surface glycosylation on the HIV-1 envelope (Env) protein	102:164	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	3	47	theme	HIV-1	397:401	arg1	Env					403:405	fully glycosylated HIV-1 Env	378:405	fully glycosylated HIV-1 Env with graph theory	378:423	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33319171	6	48	theme	protein	989:995	arg1	surface					997:1003	the antigenic protein surface	975:1003	the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design	975:1130	We have also derived a measure to quantify the shielding effect over the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design.					
33319171	0	49	dep	Resilience	22:31	arg1	the					18:20	the	18:20	the	18:20	Quantification of the Resilience and Vulnerability of HIV-1 Native Glycan Shield at Atomistic Detail.					
33319171	2	50	theme	molecular	229:237	arg1	complexity					239:248	the molecular complexity	225:248	the molecular complexity	225:248	However, the molecular complexity and flexibility of glycans make experimental studies a challenge.					
33319171	1	51	theme	adaptive	192:199	arg1	system					208:213	the adaptive immune system	188:213	the adaptive immune system	188:213	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	4	52	dep	glycan	674:679	arg1	shield					681:686	shield	681:686	shield	681:686	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	3	53	theme	shield	478:483	arg1	topology					485:492	the shield topology	474:492	the shield topology	474:492	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33319171	1	54	theme	immune	201:206	arg1	system					208:213	the adaptive immune system	188:213	the adaptive immune system	188:213	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	0	55	theme	Glycan	67:72	arg1	Vulnerability					37:49	Vulnerability	37:49	Vulnerability	37:49	Quantification of the Resilience and Vulnerability of HIV-1 Native Glycan Shield at Atomistic Detail.					
33319171	0	55	theme	Glycan	67:72	arg1	Resilience					22:31	Resilience	22:31	Resilience	22:31	Quantification of the Resilience and Vulnerability of HIV-1 Native Glycan Shield at Atomistic Detail.					
33319171	1	56	theme	HIV-1	137:141	arg1	protein					158:164	the HIV-1 envelope (Env) protein	133:164	the HIV-1 envelope (Env) protein	133:164	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	0	57	theme	Native	60:65	arg1	Glycan					67:72	HIV-1 Native Glycan	54:72	HIV-1 Native Glycan	54:72	Quantification of the Resilience and Vulnerability of HIV-1 Native Glycan Shield at Atomistic Detail.					
33319171	4	58	theme	complex	606:612	arg1	glycans					614:620	both oligomannose and complex glycans	584:620	glycans	614:620	This is the first complete all-atom model of HIV-1 Env SOSIP glycan shield that includes both oligomannose and complex glycans, providing physiologically relevant insights of the glycan shield.					
33319171	1	59	theme	envelope	143:150	arg1	protein					158:164	the HIV-1 envelope (Env) protein	133:164	the HIV-1 envelope (Env) protein	133:164	Dense surface glycosylation on the HIV-1 envelope (Env) protein acts as a shield from the adaptive immune system.					
33319171	6	60	theme	antigenic	979:987	arg1	surface					997:1003	the antigenic protein surface	975:1003	the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design	975:1130	We have also derived a measure to quantify the shielding effect over the antigenic protein surface that defines regions of relative vulnerability and resilience of the shield and can be harnessed for rational immunogen design.					
33319171	3	61	theme	high-throughput	340:354	arg1	modeling					366:373	high-throughput atomistic modeling	340:373	high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory	340:423	Here we have integrated high-throughput atomistic modeling of fully glycosylated HIV-1 Env with graph theory to capture immunologically important features of the shield topology.					
33541668	6	0	theme	strain	1050:1055	arg1	sensor					1057:1062	antibacterial strain sensor	1036:1062	antibacterial strain sensor	1036:1062	Finally, the composite hydrogels are applied to the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07.					
33541668	2	1	theme	cross-linked	367:378	arg1	hydrogel					380:387	a chemically cross-linked hydrogel	354:387	a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor	354:431	In this paper, silver nanoparticles were prepared by solid-state reduction of hydroxyethyl cellulose and compounded into a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor.					
33541668	6	2	theme	sensor	1057:1062	arg1	field					1027:1031	the field	1023:1031	the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07	1023:1109	Finally, the composite hydrogels are applied to the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07.					
33541668	6	3	theme	Factor	1086:1091	arg1	field					1027:1031	the field	1023:1031	the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07	1023:1109	Finally, the composite hydrogels are applied to the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07.					
33541668	0	4	theme	strain	109:114	arg1	hydrogel					123:130	antibacterial strain sensor hydrogel	95:130	antibacterial strain sensor hydrogel	95:130	Preparation of silver nanoparticles by solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel.					
33541668	2	5	theme	solid-state	286:296	arg1	reduction					298:306	solid-state reduction	286:306	solid-state reduction of hydroxyethyl cellulose	286:332	In this paper, silver nanoparticles were prepared by solid-state reduction of hydroxyethyl cellulose and compounded into a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor.					
33541668	6	6	theme	antibacterial	1036:1048	arg1	sensor					1057:1062	antibacterial strain sensor	1036:1062	antibacterial strain sensor	1036:1062	Finally, the composite hydrogels are applied to the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07.					
33541668	6	7	theme	composite	988:996	arg1	hydrogels					998:1006	the composite hydrogels	984:1006	the composite hydrogels	984:1006	Finally, the composite hydrogels are applied to the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07.					
33541668	0	8	theme	antibacterial	95:107	arg1	hydrogel					123:130	antibacterial strain sensor hydrogel	95:130	antibacterial strain sensor hydrogel	95:130	Preparation of silver nanoparticles by solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel.					
33541668	2	9	link	cross-linked	367:378	arg1	hydrogel					380:387	a chemically cross-linked hydrogel	354:387	a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor	354:431	In this paper, silver nanoparticles were prepared by solid-state reduction of hydroxyethyl cellulose and compounded into a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor.					
33541668	4	10	theme	nanoparticles	724:736	arg1	hydrogel					748:755	silver nanoparticles composite hydrogel	717:755	silver nanoparticles composite hydrogel	717:755	Impressively, silver nanoparticles composite hydrogel could only be successfully fabricated and triggered by the AIBN.					
33541668	7	11	theme	hydrogel	1232:1239	arg1	system					1241:1246	a hydrogel system	1230:1246	a hydrogel system for antibacterial strain sensor	1230:1278	This article proposes a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor.					
33541668	5	12	theme	composite	853:861	arg1	hydrogel					863:870	the composite hydrogel	849:870	the composite hydrogel (0.12 MPa at 704.33 % strain)	849:900	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain) was significantly improved because of dynamic crosslinking point by HEC.					
33541668	3	13	theme	surface	451:457	arg1	energy					459:464	the high surface energy	442:464	the high surface energy of silver nanoparticles	442:488	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	0	14	theme	sensor	116:121	arg1	hydrogel					123:130	antibacterial strain sensor hydrogel	95:130	antibacterial strain sensor hydrogel	95:130	Preparation of silver nanoparticles by solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel.					
33541668	5	15	theme	dynamic	940:946	arg1	point					961:965	dynamic crosslinking point	940:965	dynamic crosslinking point	940:965	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain) was significantly improved because of dynamic crosslinking point by HEC.					
33541668	6	16	theme	highest	1072:1078	arg1	GF					1094:1095	GF	1094:1095	GF	1094:1095	Finally, the composite hydrogels are applied to the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07.					
33541668	6	16	theme	highest	1072:1078	arg1	Factor					1086:1091	the highest Gauge Factor	1068:1091	the highest Gauge Factor (GF) reached 4.07	1068:1109	Finally, the composite hydrogels are applied to the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07.					
33541668	2	17	theme	cellulose	324:332	arg1	reduction					298:306	solid-state reduction	286:306	solid-state reduction of hydroxyethyl cellulose	286:332	In this paper, silver nanoparticles were prepared by solid-state reduction of hydroxyethyl cellulose and compounded into a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor.					
33541668	7	18	theme	antibacterial	1252:1264	arg1	sensor					1273:1278	antibacterial strain sensor	1252:1278	antibacterial strain sensor	1252:1278	This article proposes a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor.					
33541668	2	19	theme	antibacterial	395:407	arg1	sensor					426:431	an antibacterial, flexible strain sensor	392:431	an antibacterial, flexible strain sensor	392:431	In this paper, silver nanoparticles were prepared by solid-state reduction of hydroxyethyl cellulose and compounded into a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor.					
33541668	5	20	theme	mechanical	826:835	arg1	property					837:844	The mechanical property	822:844	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain)	822:900	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain) was significantly improved because of dynamic crosslinking point by HEC.					
33541668	2	21	theme	hydroxyethyl	311:322	arg1	cellulose					324:332	hydroxyethyl cellulose	311:332	hydroxyethyl cellulose	311:332	In this paper, silver nanoparticles were prepared by solid-state reduction of hydroxyethyl cellulose and compounded into a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor.					
33541668	3	22	theme	high	446:449	arg1	energy					459:464	the high surface energy	442:464	the high surface energy of silver nanoparticles	442:488	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	5	23	from	strain	894:899	arg1	MPa					878:880	0.12 MPa	873:880	0.12 MPa	873:880	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain) was significantly improved because of dynamic crosslinking point by HEC.					
33541668	3	24	theme	ammonium	570:577	arg1	persulfate					579:588	ammonium persulfate	570:588	ammonium persulfate (APS)	570:594	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	3	24	theme	ammonium	570:577	arg1	APS					591:593	APS	591:593	APS	591:593	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	1	25	theme	biocompatible	186:198	arg1	hydrogel					200:207	the biocompatible hydrogel	182:207	the biocompatible hydrogel	182:207	As a smart wearable sensor device, the mildew of the biocompatible hydrogel limits its application.					
33541668	0	26	theme	silver	15:20	arg1	Preparation					0:10	Preparation	0:10	Preparation of silver	0:20	Preparation of silver nanoparticles by solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel.					
33541668	5	27	theme	crosslinking	948:959	arg1	point					961:965	dynamic crosslinking point	940:965	dynamic crosslinking point	940:965	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain) was significantly improved because of dynamic crosslinking point by HEC.					
33541668	3	28	theme	2,2'-azobis	643:653	arg1	AIBA					696:699	AIBA	696:699	AIBA	696:699	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	3	28	theme	2,2'-azobis	643:653	arg1	dihydrochloride					679:693	2,2'-azobis(2-methylpropionamidine) dihydrochloride	643:693	2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA)	643:700	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	1	29	theme	hydrogel	200:207	arg1	mildew					172:177	the mildew	168:177	the mildew of the biocompatible hydrogel	168:207	As a smart wearable sensor device, the mildew of the biocompatible hydrogel limits its application.					
33541668	0	30	theme	solid-state	39:49	arg1	route					57:61	solid-state redox route	39:61	solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel	39:130	Preparation of silver nanoparticles by solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel.					
33541668	7	31	dep	compounding	1208:1218	arg1	them					1220:1223	them	1220:1223	them	1220:1223	This article proposes a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor.					
33541668	0	32	from	cellulose	81:89	arg1	route					57:61	solid-state redox route	39:61	solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel	39:130	Preparation of silver nanoparticles by solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel.					
33541668	5	33	theme	704.33	885:890	arg1	%					892:892	%	892:892	%	892:892	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain) was significantly improved because of dynamic crosslinking point by HEC.					
33541668	5	34	theme	hydrogel	863:870	arg1	property					837:844	The mechanical property	822:844	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain)	822:900	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain) was significantly improved because of dynamic crosslinking point by HEC.					
33541668	6	35	theme	Gauge	1080:1084	arg1	GF					1094:1095	GF	1094:1095	GF	1094:1095	Finally, the composite hydrogels are applied to the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07.					
33541668	6	35	theme	Gauge	1080:1084	arg1	Factor					1086:1091	the highest Gauge Factor	1068:1091	the highest Gauge Factor (GF) reached 4.07	1068:1109	Finally, the composite hydrogels are applied to the field of antibacterial strain sensor and the highest Gauge Factor (GF) reached 4.07.					
33541668	7	36	theme	novel	1136:1140	arg1	strategy					1160:1167	a novel, green and simple strategy	1134:1167	a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor	1134:1278	This article proposes a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor.					
33541668	2	37	theme	strain	419:424	arg1	sensor					426:431	an antibacterial, flexible strain sensor	392:431	an antibacterial, flexible strain sensor	392:431	In this paper, silver nanoparticles were prepared by solid-state reduction of hydroxyethyl cellulose and compounded into a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor.					
33541668	5	38	theme	%	892:892	arg1	strain					894:899	704.33 % strain	885:899	704.33 % strain	885:899	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain) was significantly improved because of dynamic crosslinking point by HEC.					
33541668	4	39	theme	silver	717:722	arg1	hydrogel					748:755	silver nanoparticles composite hydrogel	717:755	silver nanoparticles composite hydrogel	717:755	Impressively, silver nanoparticles composite hydrogel could only be successfully fabricated and triggered by the AIBN.					
33541668	0	40	theme	redox	51:55	arg1	route					57:61	solid-state redox route	39:61	solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel	39:130	Preparation of silver nanoparticles by solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel.					
33541668	7	41	theme	strain	1266:1271	arg1	sensor					1273:1278	antibacterial strain sensor	1252:1278	antibacterial strain sensor	1252:1278	This article proposes a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor.					
33541668	5	42	dep	hydrogel	863:870	arg1	MPa					878:880	0.12 MPa	873:880	0.12 MPa	873:880	The mechanical property of the composite hydrogel (0.12 MPa at 704.33 % strain) was significantly improved because of dynamic crosslinking point by HEC.					
33541668	2	43	dep	antibacterial	395:407	arg1	flexible					410:417	flexible	410:417	flexible	410:417	In this paper, silver nanoparticles were prepared by solid-state reduction of hydroxyethyl cellulose and compounded into a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor.					
33541668	4	44	theme	composite	738:746	arg1	hydrogel					748:755	silver nanoparticles composite hydrogel	717:755	silver nanoparticles composite hydrogel	717:755	Impressively, silver nanoparticles composite hydrogel could only be successfully fabricated and triggered by the AIBN.					
33541668	3	45	theme	2-methylpropionamidine	655:676	arg1	AIBA					696:699	AIBA	696:699	AIBA	696:699	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	3	45	theme	2-methylpropionamidine	655:676	arg1	dihydrochloride					679:693	2,2'-azobis(2-methylpropionamidine) dihydrochloride	643:693	2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA)	643:700	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	2	46	theme	silver	248:253	arg1	nanoparticles					255:267	silver nanoparticles	248:267	silver nanoparticles	248:267	In this paper, silver nanoparticles were prepared by solid-state reduction of hydroxyethyl cellulose and compounded into a chemically cross-linked hydrogel as an antibacterial, flexible strain sensor.					
33541668	3	47	theme	free	501:504	arg1	radicals					506:513	free radicals	501:513	free radicals	501:513	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	0	48	theme	hydroxyethyl	68:79	arg1	cellulose					81:89	hydroxyethyl cellulose	68:89	hydroxyethyl cellulose	68:89	Preparation of silver nanoparticles by solid-state redox route from hydroxyethyl cellulose for antibacterial strain sensor hydrogel.					
33541668	3	49	theme	silver	469:474	arg1	nanoparticles					476:488	silver nanoparticles	469:488	silver nanoparticles	469:488	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	3	50	theme	nanoparticles	476:488	arg1	energy					459:464	the high surface energy	442:464	the high surface energy of silver nanoparticles	442:488	Because the high surface energy of silver nanoparticles can quench free radicals, we designed three initiators to synthesize hydrogels: ammonium persulfate (APS), 2,2'-Azobis(2-methylpropionitrile) (AIBN) and 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AIBA).					
33541668	1	51	theme	wearable	144:151	arg1	application					220:230	its application	216:230	its application	216:230	As a smart wearable sensor device, the mildew of the biocompatible hydrogel limits its application.					
33541668	1	51	theme	wearable	144:151	arg1	device					160:165	a smart wearable sensor device	136:165	a smart wearable sensor device	136:165	As a smart wearable sensor device, the mildew of the biocompatible hydrogel limits its application.					
33541668	7	52	theme	green	1143:1147	arg1	strategy					1160:1167	a novel, green and simple strategy	1134:1167	a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor	1134:1278	This article proposes a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor.					
33541668	1	53	theme	sensor	153:158	arg1	application					220:230	its application	216:230	its application	216:230	As a smart wearable sensor device, the mildew of the biocompatible hydrogel limits its application.					
33541668	1	53	theme	sensor	153:158	arg1	device					160:165	a smart wearable sensor device	136:165	a smart wearable sensor device	136:165	As a smart wearable sensor device, the mildew of the biocompatible hydrogel limits its application.					
33541668	7	54	theme	simple	1153:1158	arg1	strategy					1160:1167	a novel, green and simple strategy	1134:1167	a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor	1134:1278	This article proposes a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor.					
33541668	7	55	theme	silver	1183:1188	arg1	nanoparticles					1190:1202	silver nanoparticles	1183:1202	silver nanoparticles	1183:1202	This article proposes a novel, green and simple strategy for preparing silver nanoparticles and compounding them into a hydrogel system for antibacterial strain sensor.					
32194116	6	0	dep	significantly	850:862	arg1	P > .05					865:871	P > .05	865:871	P > .05	865:871	Heated GSF3 did not significantly (P > .05) decreased its immunomodulatory activity, suggesting that GSF3 is a proteopolysaccharide.					
32194116	1	1	theme	fraction	318:325	arg1	property					281:288	the property	277:288	the property of each individual isolated fraction	277:325	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	1	2	theme	guava	140:144	arg1	GSPS					167:170	GSPS	167:170	GSPS	167:170	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	1	2	theme	guava	140:144	arg1	polysaccharide					151:164	a novel guava seed polysaccharide	132:164	a novel guava seed polysaccharide (GSPS)	132:171	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	1	3	theme	6B	219:220	arg1	chromatography					237:250	Sepharose 6B gel filtration chromatography	209:250	Sepharose 6B gel filtration chromatography	209:250	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	1	4	theme	seed	146:149	arg1	GSPS					167:170	GSPS	167:170	GSPS	167:170	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	1	4	theme	seed	146:149	arg1	polysaccharide					151:164	a novel guava seed polysaccharide	132:164	a novel guava seed polysaccharide (GSPS)	132:171	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	1	5	theme	gel	222:224	arg1	chromatography					237:250	Sepharose 6B gel filtration chromatography	209:250	Sepharose 6B gel filtration chromatography	209:250	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	8	6	theme	monosaccharides	1152:1166	arg1	composition					1168:1178	monosaccharides composition	1152:1178	monosaccharides composition	1152:1178	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	3	7	theme	high	501:504	arg1	chromatography					533:546	high performance size exclusion chromatography	501:546	high performance size exclusion chromatography	501:546	Isolated GSF1, GSF2 and GSF3 were respectively subjected to high performance size exclusion chromatography; molecular weights of three polysaccharide fractions were determined.					
32194116	1	8	theme	filtration	226:235	arg1	chromatography					237:250	Sepharose 6B gel filtration chromatography	209:250	Sepharose 6B gel filtration chromatography	209:250	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	0	9	theme	immunomodulatory	78:93	arg1	activity					95:102	an immunomodulatory activity	75:102	an immunomodulatory activity	75:102	Characterization of guava (Psidium guajava Linn) seed polysaccharides with an immunomodulatory activity.					
32194116	0	10	with	polysaccharides	54:68	arg1	activity					95:102	an immunomodulatory activity	75:102	an immunomodulatory activity	75:102	Characterization of guava (Psidium guajava Linn) seed polysaccharides with an immunomodulatory activity.					
32194116	3	11	theme	performance	506:516	arg1	chromatography					533:546	high performance size exclusion chromatography	501:546	high performance size exclusion chromatography	501:546	Isolated GSF1, GSF2 and GSF3 were respectively subjected to high performance size exclusion chromatography; molecular weights of three polysaccharide fractions were determined.					
32194116	9	12	with	proteopolysaccharide	1551:1570	arg1	anti-inflammatory					1584:1600	anti-inflammatory	1584:1600	anti-inflammatory	1584:1600	Overall, we evidence that GSF3 is a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects.					
32194116	9	12	with	proteopolysaccharide	1551:1570	arg1	effects					1623:1629	immunomodulatory effects	1606:1629	immunomodulatory effects	1606:1629	Overall, we evidence that GSF3 is a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects.					
32194116	1	13	theme	polysaccharide	151:164	arg1	property					120:127	the property	116:127	the property of a novel guava seed polysaccharide (GSPS)	116:171	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	8	14	theme	composition	1168:1178	arg1	Analyses					1140:1147	Analyses	1140:1147	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).	1140:1492	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	7	15	theme	carbohydrate	1064:1075	arg1	moiety					1077:1082	carbohydrate moiety	1064:1082	carbohydrate moiety in GSF3	1064:1090	The deproteinated GSF3 markedly lost its immunomodulatory activity, suggesting that both protein and carbohydrate moiety in GSF3 are essential to its immunomodulatory function.					
32194116	9	16	theme	proteopolysaccharide	1551:1570	arg1	GSF3					1520:1523	GSF3	1520:1523	GSF3	1520:1523	Overall, we evidence that GSF3 is a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects.					
32194116	9	16	theme	proteopolysaccharide	1551:1570	arg1	weight					1544:1549	a low molecular weight	1528:1549	a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects	1528:1629	Overall, we evidence that GSF3 is a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects.					
32194116	6	17	theme	immunomodulatory	888:903	arg1	activity					905:912	its immunomodulatory activity	884:912	its immunomodulatory activity	884:912	Heated GSF3 did not significantly (P > .05) decreased its immunomodulatory activity, suggesting that GSF3 is a proteopolysaccharide.					
32194116	2	18	theme	purified	361:368	arg1	fraction					407:414	guava seed polysaccharide fraction 1	381:416	guava seed polysaccharide fraction 1 (GSF1)	381:423	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	2	18	theme	purified	361:368	arg1	GSF3					435:438	GSF3	435:438	GSF3	435:438	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	2	18	theme	purified	361:368	arg1	fractions					370:378	three purified fractions	355:378	three purified fractions	355:378	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	2	18	theme	purified	361:368	arg1	GSF2					426:429	GSF2	426:429	GSF2	426:429	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	8	19	theme	pre-column	1196:1205	arg1	chromatography					1246:1259	a pre-column derivatization high performance liquid chromatography	1194:1259	a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%)	1194:1491	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	7	20	from	moiety	1077:1082	arg1	GSF3					1087:1090	GSF3	1087:1090	GSF3	1087:1090	The deproteinated GSF3 markedly lost its immunomodulatory activity, suggesting that both protein and carbohydrate moiety in GSF3 are essential to its immunomodulatory function.					
32194116	9	21	theme	immunomodulatory	1606:1621	arg1	effects					1623:1629	immunomodulatory effects	1606:1629	immunomodulatory effects	1606:1629	Overall, we evidence that GSF3 is a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects.					
32194116	3	22	theme	size	518:521	arg1	chromatography					533:546	high performance size exclusion chromatography	501:546	high performance size exclusion chromatography	501:546	Isolated GSF1, GSF2 and GSF3 were respectively subjected to high performance size exclusion chromatography; molecular weights of three polysaccharide fractions were determined.					
32194116	5	23	theme	Th2-inclination	772:786	arg1	property					788:795	a Th2-inclination property	770:795	a Th2-inclination property	770:795	GSPS, GSF1, GSF2 and GSF3, particularly GSF3, were found to have a Th2-inclination property and anti-inflammatory potential.					
32194116	0	24	theme	guava	20:24	arg1	Characterization					0:15	Characterization	0:15	Characterization of guava (Psidium guajava Linn)	0:47	Characterization of guava (Psidium guajava Linn) seed polysaccharides with an immunomodulatory activity.					
32194116	8	25	from	Analyses	1140:1147	arg1	GSF3					1183:1186	GSF3	1183:1186	GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%)	1183:1491	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	3	26	theme	exclusion	523:531	arg1	chromatography					533:546	high performance size exclusion chromatography	501:546	high performance size exclusion chromatography	501:546	Isolated GSF1, GSF2 and GSF3 were respectively subjected to high performance size exclusion chromatography; molecular weights of three polysaccharide fractions were determined.					
32194116	8	27	theme	high	1222:1225	arg1	chromatography					1246:1259	a pre-column derivatization high performance liquid chromatography	1194:1259	a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%)	1194:1491	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	28	theme	galacturonic	1322:1333	arg1	%					1346:1346	28.13%	1341:1346	28.13%	1341:1346	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	28	theme	galacturonic	1322:1333	arg1	acid					1308:1311	glucuronic acid	1297:1311	glucuronic acid (3.28%)	1297:1319	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	28	theme	galacturonic	1322:1333	arg1	acid					1335:1338	galacturonic acid	1322:1338	galacturonic acid (28.13%)	1322:1347	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	4	29	gly	glycoproteins	690:702	arg1	glycoproteins					690:702	glycoproteins	690:702	glycoproteins	690:702	GSPS, GSF1, GSF2 and GSF3 were suggested to be proteopolysaccharides or glycoproteins.					
32194116	5	30	contain	have	765:768	arg2	potential					819:827	anti-inflammatory potential	801:827	anti-inflammatory potential	801:827	GSPS, GSF1, GSF2 and GSF3, particularly GSF3, were found to have a Th2-inclination property and anti-inflammatory potential.					
32194116	5	30	contain	have	765:768	arg1	GSF2					717:720	GSF2	717:720	GSF2	717:720	GSPS, GSF1, GSF2 and GSF3, particularly GSF3, were found to have a Th2-inclination property and anti-inflammatory potential.					
32194116	5	30	contain	have	765:768	arg1	GSF3					726:729	GSF3	726:729	GSF3	726:729	GSPS, GSF1, GSF2 and GSF3, particularly GSF3, were found to have a Th2-inclination property and anti-inflammatory potential.					
32194116	5	30	contain	have	765:768	arg1	GSPS					705:708	GSPS	705:708	GSPS	705:708	GSPS, GSF1, GSF2 and GSF3, particularly GSF3, were found to have a Th2-inclination property and anti-inflammatory potential.					
32194116	5	30	contain	have	765:768	arg2	property					788:795	a Th2-inclination property	770:795	a Th2-inclination property	770:795	GSPS, GSF1, GSF2 and GSF3, particularly GSF3, were found to have a Th2-inclination property and anti-inflammatory potential.					
32194116	5	30	contain	have	765:768	arg1	GSF1					711:714	GSF1	711:714	GSF1	711:714	GSPS, GSF1, GSF2 and GSF3, particularly GSF3, were found to have a Th2-inclination property and anti-inflammatory potential.					
32194116	2	31	theme	polysaccharide	392:405	arg1	fraction					407:414	guava seed polysaccharide fraction 1	381:416	guava seed polysaccharide fraction 1 (GSF1)	381:423	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	2	31	theme	polysaccharide	392:405	arg1	GSF1					419:422	GSF1	419:422	GSF1	419:422	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	2	31	theme	polysaccharide	392:405	arg1	fractions					370:378	three purified fractions	355:378	three purified fractions	355:378	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	2	32	theme	guava	381:385	arg1	fraction					407:414	guava seed polysaccharide fraction 1	381:416	guava seed polysaccharide fraction 1 (GSF1)	381:423	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	2	32	theme	guava	381:385	arg1	GSF1					419:422	GSF1	419:422	GSF1	419:422	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	2	32	theme	guava	381:385	arg1	fractions					370:378	three purified fractions	355:378	three purified fractions	355:378	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	8	33	theme	derivatization	1207:1220	arg1	chromatography					1246:1259	a pre-column derivatization high performance liquid chromatography	1194:1259	a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%)	1194:1491	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	ribose					1424:1429	ribose	1424:1429	ribose (1.55%)	1424:1437	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	mannose					1370:1376	mannose	1370:1376	mannose (3.96%)	1370:1384	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	glucose					1387:1393	glucose	1387:1393	glucose (22.99%)	1387:1402	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	acid					1335:1338	galacturonic acid	1322:1338	galacturonic acid (28.13%)	1322:1347	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	galactose					1350:1358	galactose	1350:1358	galactose (14.88%)	1350:1367	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	acid					1308:1311	glucuronic acid	1297:1311	glucuronic acid (3.28%)	1297:1319	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	%					1318:1318	3.28%	1314:1318	3.28%	1314:1318	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	rhamnose					1476:1483	rhamnose	1476:1483	rhamnose	1476:1483	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	xylose					1440:1445	xylose	1440:1445	xylose (14.81%)	1440:1454	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	fucose					1457:1462	fucose	1457:1462	fucose (1.68%)	1457:1470	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	8	34	theme	glucuronic	1297:1306	arg1	arabinose					1405:1413	arabinose	1405:1413	arabinose (7.31%)	1405:1421	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	2	35	theme	seed	387:390	arg1	fraction					407:414	guava seed polysaccharide fraction 1	381:416	guava seed polysaccharide fraction 1 (GSF1)	381:423	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	2	35	theme	seed	387:390	arg1	GSF1					419:422	GSF1	419:422	GSF1	419:422	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	2	35	theme	seed	387:390	arg1	fractions					370:378	three purified fractions	355:378	three purified fractions	355:378	GSPS further resolved into three purified fractions, guava seed polysaccharide fraction 1 (GSF1), GSF2 and GSF3.					
32194116	7	36	theme	immunomodulatory	1113:1128	arg1	function					1130:1137	its immunomodulatory function	1109:1137	its immunomodulatory function	1109:1137	The deproteinated GSF3 markedly lost its immunomodulatory activity, suggesting that both protein and carbohydrate moiety in GSF3 are essential to its immunomodulatory function.					
32194116	3	37	theme	molecular	549:557	arg1	weights					559:565	molecular weights	549:565	molecular weights of three polysaccharide fractions	549:599	Isolated GSF1, GSF2 and GSF3 were respectively subjected to high performance size exclusion chromatography; molecular weights of three polysaccharide fractions were determined.					
32194116	9	38	theme	molecular	1534:1542	arg1	GSF3					1520:1523	GSF3	1520:1523	GSF3	1520:1523	Overall, we evidence that GSF3 is a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects.					
32194116	9	38	theme	molecular	1534:1542	arg1	weight					1544:1549	a low molecular weight	1528:1549	a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects	1528:1629	Overall, we evidence that GSF3 is a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects.					
32194116	8	39	theme	performance	1227:1237	arg1	chromatography					1246:1259	a pre-column derivatization high performance liquid chromatography	1194:1259	a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%)	1194:1491	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	9	40	theme	low	1530:1532	arg1	GSF3					1520:1523	GSF3	1520:1523	GSF3	1520:1523	Overall, we evidence that GSF3 is a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects.					
32194116	9	40	theme	low	1530:1532	arg1	weight					1544:1549	a low molecular weight	1528:1549	a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects	1528:1629	Overall, we evidence that GSF3 is a low molecular weight proteopolysaccharide with potent anti-inflammatory and immunomodulatory effects.					
32194116	1	41	theme	individual	298:307	arg1	fraction					318:325	each individual isolated fraction	293:325	each individual isolated fraction	293:325	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	7	42	theme	immunomodulatory	1004:1019	arg1	activity					1021:1028	its immunomodulatory activity	1000:1028	its immunomodulatory activity	1000:1028	The deproteinated GSF3 markedly lost its immunomodulatory activity, suggesting that both protein and carbohydrate moiety in GSF3 are essential to its immunomodulatory function.					
32194116	3	43	theme	Isolated	441:448	arg1	GSF1					450:453	GSF1	450:453	GSF1	450:453	Isolated GSF1, GSF2 and GSF3 were respectively subjected to high performance size exclusion chromatography; molecular weights of three polysaccharide fractions were determined.					
32194116	8	44	theme	liquid	1239:1244	arg1	chromatography					1246:1259	a pre-column derivatization high performance liquid chromatography	1194:1259	a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%)	1194:1491	Analyses of monosaccharides composition in GSF3 using a pre-column derivatization high performance liquid chromatography exhibited that GSF3 was composed of glucuronic acid (3.28%), galacturonic acid (28.13%), galactose (14.88%), mannose (3.96%), glucose (22.99%), arabinose (7.31%), ribose (1.55%), xylose (14.81%), fucose (1.68%) and rhamnose (1.43%).					
32194116	6	45	theme	Heated	830:835	arg1	GSF3					837:840	Heated GSF3	830:840	Heated GSF3	830:840	Heated GSF3 did not significantly (P > .05) decreased its immunomodulatory activity, suggesting that GSF3 is a proteopolysaccharide.					
32194116	5	46	theme	anti-inflammatory	801:817	arg1	potential					819:827	anti-inflammatory potential	801:827	anti-inflammatory potential	801:827	GSPS, GSF1, GSF2 and GSF3, particularly GSF3, were found to have a Th2-inclination property and anti-inflammatory potential.					
32194116	3	47	theme	polysaccharide	576:589	arg1	fractions					591:599	three polysaccharide fractions	570:599	three polysaccharide fractions	570:599	Isolated GSF1, GSF2 and GSF3 were respectively subjected to high performance size exclusion chromatography; molecular weights of three polysaccharide fractions were determined.					
32194116	7	48	theme	deproteinated	967:979	arg1	GSF3					981:984	The deproteinated GSF3	963:984	The deproteinated GSF3	963:984	The deproteinated GSF3 markedly lost its immunomodulatory activity, suggesting that both protein and carbohydrate moiety in GSF3 are essential to its immunomodulatory function.					
32194116	7	49	from	protein	1052:1058	arg1	GSF3					1087:1090	GSF3	1087:1090	GSF3	1087:1090	The deproteinated GSF3 markedly lost its immunomodulatory activity, suggesting that both protein and carbohydrate moiety in GSF3 are essential to its immunomodulatory function.					
32194116	1	50	theme	isolated	309:316	arg1	fraction					318:325	each individual isolated fraction	293:325	each individual isolated fraction	293:325	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	3	51	theme	fractions	591:599	arg1	weights					559:565	molecular weights	549:565	molecular weights of three polysaccharide fractions	549:599	Isolated GSF1, GSF2 and GSF3 were respectively subjected to high performance size exclusion chromatography; molecular weights of three polysaccharide fractions were determined.					
32194116	1	52	theme	novel	134:138	arg1	GSPS					167:170	GSPS	167:170	GSPS	167:170	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	1	52	theme	novel	134:138	arg1	polysaccharide					151:164	a novel guava seed polysaccharide	132:164	a novel guava seed polysaccharide (GSPS)	132:171	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
32194116	1	53	theme	Sepharose	209:217	arg1	chromatography					237:250	Sepharose 6B gel filtration chromatography	209:250	Sepharose 6B gel filtration chromatography	209:250	To clarify the property of a novel guava seed polysaccharide (GSPS), GSPS was subjected to purify using Sepharose 6B gel filtration chromatography and further characterize the property of each individual isolated fraction.					
31987946	7	0	theme	polysaccharides	1265:1279	arg1	actions					1254:1260	the synergistic actions	1238:1260	the synergistic actions of polysaccharides with small molecules	1238:1300	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	5	1	theme	weight	936:941	arg1	polysaccharide					943:956	high molecular weight polysaccharide	921:956	high molecular weight polysaccharide	921:956	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	0	2	theme	gut	81:83	arg1	microbiota					85:94	the gut microbiota	77:94	the gut microbiota	77:94	Cistanche polysaccharides enhance echinacoside absorption in vivo and affect the gut microbiota.					
31987946	2	3	from	effects	466:472	arg1	microbiota					500:509	gut microbiota	496:509	gut microbiota	496:509	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	7	4	theme	microbiota	1432:1441	arg1	manipulator					1443:1453	a gut microbiota manipulator	1426:1453	a gut microbiota manipulator for health promotion	1426:1474	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	4	theme	microbiota	1432:1441	arg1	polysaccharides					1341:1355	cistanche polysaccharides	1331:1355	cistanche polysaccharides	1331:1355	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	3	5	theme	different	546:554	arg1	kinds					556:560	different kinds	546:560	different kinds of cistanche polysaccharides	546:589	Sprague-Dawley rats were fed with different kinds of cistanche polysaccharides for 20 days, on the last day, all rats were administered the echinacoside at 100 mg/kg.					
31987946	3	6	theme	polysaccharides	575:589	arg1	kinds					556:560	different kinds	546:560	different kinds of cistanche polysaccharides	546:589	Sprague-Dawley rats were fed with different kinds of cistanche polysaccharides for 20 days, on the last day, all rats were administered the echinacoside at 100 mg/kg.					
31987946	1	7	theme	compounds	285:293	arg1	kinds					276:280	these two kinds	266:280	these two kinds of compounds	266:293	The polysaccharides and phenylethanoid glycosides from Cistanche deserticola have been demonstrated with various health benefits, however the interactive effect between these two kinds of compounds in vivo are not in detail known.					
31987946	1	8	from	deserticola	162:172	arg1	polysaccharides					101:115	polysaccharides	101:115	polysaccharides	101:115	The polysaccharides and phenylethanoid glycosides from Cistanche deserticola have been demonstrated with various health benefits, however the interactive effect between these two kinds of compounds in vivo are not in detail known.					
31987946	1	8	from	deserticola	162:172	arg1	glycosides					136:145	phenylethanoid glycosides	121:145	phenylethanoid glycosides	121:145	The polysaccharides and phenylethanoid glycosides from Cistanche deserticola have been demonstrated with various health benefits, however the interactive effect between these two kinds of compounds in vivo are not in detail known.					
31987946	5	9	theme	gut	1018:1020	arg1	diversity					1033:1041	the gut microbiota diversity	1014:1041	the gut microbiota diversity	1014:1041	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	4	10	theme	parameters	749:758	arg1	difference					719:728	the difference	715:728	the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents	715:824	The results were compared mainly on the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents.					
31987946	2	11	theme	study	350:354	arg1	objective					332:340	The objective	328:340	The objective of this study	328:354	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	5	12	theme	cistanche	862:870	arg1	polysaccharides					872:886	all the cistanche polysaccharides	854:886	all the cistanche polysaccharides	854:886	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	5	12	theme	cistanche	862:870	arg1	polysaccharide					983:996	low molecular weight polysaccharide	962:996	low molecular weight polysaccharide	962:996	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	5	12	theme	cistanche	862:870	arg1	polysaccharide					943:956	high molecular weight polysaccharide	921:956	high molecular weight polysaccharide	921:956	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	5	12	theme	cistanche	862:870	arg1	polysaccharide					905:918	crude polysaccharide	899:918	crude polysaccharide	899:918	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	4	13	theme	fatty	805:809	arg1	acids					811:815	short chain fatty acids	793:815	short chain fatty acids	793:815	The results were compared mainly on the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents.					
31987946	2	14	theme	cistanche	402:410	arg1	polysaccharides					412:426	cistanche polysaccharides	402:426	cistanche polysaccharides	402:426	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	7	15	theme	synergistic	1242:1252	arg1	actions					1254:1260	the synergistic actions	1238:1260	the synergistic actions of polysaccharides with small molecules	1238:1300	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	16	theme	weight	1385:1390	arg1	polysaccharides					1341:1355	cistanche polysaccharides	1331:1355	cistanche polysaccharides	1331:1355	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	16	theme	weight	1385:1390	arg1	polysaccharides					1392:1406	particularly low molecular weight polysaccharides	1358:1406	particularly low molecular weight polysaccharides	1358:1406	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	3	17	theme	cistanche	565:573	arg1	polysaccharides					575:589	cistanche polysaccharides	565:589	cistanche polysaccharides	565:589	Sprague-Dawley rats were fed with different kinds of cistanche polysaccharides for 20 days, on the last day, all rats were administered the echinacoside at 100 mg/kg.					
31987946	1	18	theme	various	202:208	arg1	benefits					217:224	various health benefits	202:224	various health benefits	202:224	The polysaccharides and phenylethanoid glycosides from Cistanche deserticola have been demonstrated with various health benefits, however the interactive effect between these two kinds of compounds in vivo are not in detail known.					
31987946	7	19	theme	gut	1428:1430	arg1	manipulator					1443:1453	a gut microbiota manipulator	1426:1453	a gut microbiota manipulator for health promotion	1426:1474	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	19	theme	gut	1428:1430	arg1	polysaccharides					1341:1355	cistanche polysaccharides	1331:1355	cistanche polysaccharides	1331:1355	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	4	20	theme	microbiota	765:774	arg1	composition					776:786	gut microbiota composition	761:786	gut microbiota composition	761:786	The results were compared mainly on the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents.					
31987946	7	21	theme	cistanche	1331:1339	arg1	manipulator					1443:1453	a gut microbiota manipulator	1426:1453	a gut microbiota manipulator for health promotion	1426:1474	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	21	theme	cistanche	1331:1339	arg1	polysaccharides					1341:1355	cistanche polysaccharides	1331:1355	cistanche polysaccharides	1331:1355	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	21	theme	cistanche	1331:1339	arg1	polysaccharides					1392:1406	particularly low molecular weight polysaccharides	1358:1406	particularly low molecular weight polysaccharides	1358:1406	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	1	22	theme	health	210:215	arg1	benefits					217:224	various health benefits	202:224	various health benefits	202:224	The polysaccharides and phenylethanoid glycosides from Cistanche deserticola have been demonstrated with various health benefits, however the interactive effect between these two kinds of compounds in vivo are not in detail known.					
31987946	0	23	theme	Cistanche	0:8	arg1	polysaccharides					10:24	Cistanche polysaccharides	0:24	Cistanche polysaccharides	0:24	Cistanche polysaccharides enhance echinacoside absorption in vivo and affect the gut microbiota.					
31987946	5	24	theme	spp	1123:1125	arg1	growth					1102:1107	the growth	1098:1107	the growth of Prevotella spp	1098:1125	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	4	25	theme	gut	761:763	arg1	composition					776:786	gut microbiota composition	761:786	gut microbiota composition	761:786	The results were compared mainly on the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents.					
31987946	4	26	theme	short	793:797	arg1	acids					811:815	short chain fatty acids	793:815	short chain fatty acids	793:815	The results were compared mainly on the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents.					
31987946	2	27	theme	synergistic	379:389	arg1	actions					391:397	the synergistic actions	375:397	the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside	375:456	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	0	28	theme	echinacoside	34:45	arg1	absorption					47:56	echinacoside absorption	34:56	echinacoside absorption	34:56	Cistanche polysaccharides enhance echinacoside absorption in vivo and affect the gut microbiota.					
31987946	5	29	theme	weight	976:981	arg1	polysaccharide					983:996	low molecular weight polysaccharide	962:996	low molecular weight polysaccharide	962:996	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	7	30	theme	molecular	1375:1383	arg1	polysaccharides					1341:1355	cistanche polysaccharides	1331:1355	cistanche polysaccharides	1331:1355	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	30	theme	molecular	1375:1383	arg1	polysaccharides					1392:1406	particularly low molecular weight polysaccharides	1358:1406	particularly low molecular weight polysaccharides	1358:1406	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	4	31	dep	parameters	749:758	arg1	contents					817:824	contents	817:824	contents	817:824	The results were compared mainly on the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents.					
31987946	5	32	theme	microbiota	1022:1031	arg1	diversity					1033:1041	the gut microbiota diversity	1014:1041	the gut microbiota diversity	1014:1041	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	4	33	theme	composition	776:786	arg1	difference					719:728	the difference	715:728	the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents	715:824	The results were compared mainly on the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents.					
31987946	2	34	theme	phenylethanoid	433:446	arg1	glycoside					448:456	phenylethanoid glycoside	433:456	phenylethanoid glycoside	433:456	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	7	35	theme	low	1371:1373	arg1	polysaccharides					1341:1355	cistanche polysaccharides	1331:1355	cistanche polysaccharides	1331:1355	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	35	theme	low	1371:1373	arg1	polysaccharides					1392:1406	particularly low molecular weight polysaccharides	1358:1406	particularly low molecular weight polysaccharides	1358:1406	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	6	36	theme	fatty	1177:1181	arg1	acids					1183:1187	short chain fatty acids	1165:1187	short chain fatty acids	1165:1187	as well as improve the production of short chain fatty acids and the absorption of echinacoside.					
31987946	2	37	theme	gut	496:498	arg1	microbiota					500:509	gut microbiota	496:509	gut microbiota	496:509	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	7	38	used	used	1418:1421	arg2	manipulator					1443:1453	a gut microbiota manipulator	1426:1453	a gut microbiota manipulator for health promotion	1426:1474	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	38	used	used	1418:1421	arg2	polysaccharides					1341:1355	cistanche polysaccharides	1331:1355	cistanche polysaccharides	1331:1355	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	38	used	used	1418:1421	arg2	polysaccharides					1392:1406	particularly low molecular weight polysaccharides	1358:1406	particularly low molecular weight polysaccharides	1358:1406	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	7	39	theme	health	1459:1464	arg1	promotion					1466:1474	health promotion	1459:1474	health promotion	1459:1474	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	2	40	from	actions	391:397	arg1	microbiota					500:509	gut microbiota	496:509	gut microbiota	496:509	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	1	41	theme	interactive	239:249	arg1	effect					251:256	the interactive effect	235:256	the interactive effect between these two kinds of compounds in vivo	235:301	The polysaccharides and phenylethanoid glycosides from Cistanche deserticola have been demonstrated with various health benefits, however the interactive effect between these two kinds of compounds in vivo are not in detail known.					
31987946	5	42	theme	high	921:924	arg1	polysaccharide					943:956	high molecular weight polysaccharide	921:956	high molecular weight polysaccharide	921:956	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	3	43	theme	last	611:614	arg1	day					616:618	the last day	607:618	the last day	607:618	Sprague-Dawley rats were fed with different kinds of cistanche polysaccharides for 20 days, on the last day, all rats were administered the echinacoside at 100 mg/kg.					
31987946	1	44	theme	phenylethanoid	121:134	arg1	glycosides					136:145	phenylethanoid glycosides	121:145	phenylethanoid glycosides	121:145	The polysaccharides and phenylethanoid glycosides from Cistanche deserticola have been demonstrated with various health benefits, however the interactive effect between these two kinds of compounds in vivo are not in detail known.					
31987946	2	45	theme	polysaccharides	477:491	arg1	effects					466:472	the effects	462:472	the effects of polysaccharides on gut microbiota	462:509	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	2	45	theme	polysaccharides	477:491	arg1	actions					391:397	the synergistic actions	375:397	the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside	375:456	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	5	46	theme	low	962:964	arg1	polysaccharide					983:996	low molecular weight polysaccharide	962:996	low molecular weight polysaccharide	962:996	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	4	47	theme	pharmacokinetic	733:747	arg1	parameters					749:758	pharmacokinetic parameters	733:758	pharmacokinetic parameters	733:758	The results were compared mainly on the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents.					
31987946	7	48	with	polysaccharides	1265:1279	arg1	molecules					1292:1300	small molecules	1286:1300	small molecules	1286:1300	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	2	49	with	effects	466:472	arg1	glycoside					448:456	phenylethanoid glycoside	433:456	phenylethanoid glycoside	433:456	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	5	50	theme	molecular	966:974	arg1	polysaccharide					983:996	low molecular weight polysaccharide	962:996	low molecular weight polysaccharide	962:996	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	6	51	theme	short	1165:1169	arg1	acids					1183:1187	short chain fatty acids	1165:1187	short chain fatty acids	1165:1187	as well as improve the production of short chain fatty acids and the absorption of echinacoside.					
31987946	4	52	theme	chain	799:803	arg1	acids					811:815	short chain fatty acids	793:815	short chain fatty acids	793:815	The results were compared mainly on the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents.					
31987946	4	53	theme	acids	811:815	arg1	difference					719:728	the difference	715:728	the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents	715:824	The results were compared mainly on the difference of pharmacokinetic parameters, gut microbiota composition, and short chain fatty acids contents.					
31987946	3	54	theme	Sprague-Dawley	512:525	arg1	rats					527:530	Sprague-Dawley rats	512:530	Sprague-Dawley rats	512:530	Sprague-Dawley rats were fed with different kinds of cistanche polysaccharides for 20 days, on the last day, all rats were administered the echinacoside at 100 mg/kg.					
31987946	6	55	theme	acids	1183:1187	arg1	production					1151:1160	the production	1147:1160	the production of short chain fatty acids	1147:1187	as well as improve the production of short chain fatty acids and the absorption of echinacoside.					
31987946	6	55	theme	acids	1183:1187	arg1	absorption					1197:1206	the absorption	1193:1206	the absorption of echinacoside	1193:1222	as well as improve the production of short chain fatty acids and the absorption of echinacoside.					
31987946	6	56	theme	chain	1171:1175	arg1	acids					1183:1187	short chain fatty acids	1165:1187	short chain fatty acids	1165:1187	as well as improve the production of short chain fatty acids and the absorption of echinacoside.					
31987946	7	57	theme	small	1286:1290	arg1	molecules					1292:1300	small molecules	1286:1300	small molecules	1286:1300	By exploring the synergistic actions of polysaccharides with small molecules, these findings suggest that cistanche polysaccharides, particularly low molecular weight polysaccharides, could be used as a gut microbiota manipulator for health promotion.					
31987946	2	58	theme	polysaccharides	412:426	arg1	effects					466:472	the effects	462:472	the effects of polysaccharides on gut microbiota	462:509	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	2	58	theme	polysaccharides	412:426	arg1	actions					391:397	the synergistic actions	375:397	the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside	375:456	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	5	59	theme	beneficial	1053:1062	arg1	bacteria					1064:1071	beneficial bacteria	1053:1071	beneficial bacteria	1053:1071	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	6	60	theme	echinacoside	1211:1222	arg1	production					1151:1160	the production	1147:1160	the production of short chain fatty acids	1147:1187	as well as improve the production of short chain fatty acids and the absorption of echinacoside.					
31987946	6	60	theme	echinacoside	1211:1222	arg1	absorption					1197:1206	the absorption	1193:1206	the absorption of echinacoside	1193:1222	as well as improve the production of short chain fatty acids and the absorption of echinacoside.					
31987946	1	61	dep	polysaccharides	101:115	arg1	The					97:99	The	97:99	The	97:99	The polysaccharides and phenylethanoid glycosides from Cistanche deserticola have been demonstrated with various health benefits, however the interactive effect between these two kinds of compounds in vivo are not in detail known.					
31987946	5	62	theme	crude	899:903	arg1	polysaccharide					905:918	crude polysaccharide	899:918	crude polysaccharide	899:918	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
31987946	2	63	with	actions	391:397	arg1	glycoside					448:456	phenylethanoid glycoside	433:456	phenylethanoid glycoside	433:456	The objective of this study was to investigate the synergistic actions of cistanche polysaccharides with phenylethanoid glycoside and the effects of polysaccharides on gut microbiota.					
31987946	5	64	theme	molecular	926:934	arg1	polysaccharide					943:956	high molecular weight polysaccharide	921:956	high molecular weight polysaccharide	921:956	The results indicated that all the cistanche polysaccharides, including crude polysaccharide, high molecular weight polysaccharide and low molecular weight polysaccharide, could regulate the gut microbiota diversity, increase beneficial bacteria and particularly enhance the growth of Prevotella spp.					
32050450	3	0	from	°C.	698:700	arg1	optimum					676:682	a temperature optimum	662:682	a temperature optimum from 40 to 60 °C. Kinetic studies	662:716	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	3	1	theme	temperature	664:674	arg1	optimum					676:682	a temperature optimum	662:682	a temperature optimum from 40 to 60 °C. Kinetic studies	662:716	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	7	2	theme	one-step	1443:1450	arg1	synthesis					1452:1460	the one-step synthesis	1439:1460	the one-step synthesis of alkyl glycosides with more than one monosaccharide unit	1439:1519	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	0	3	theme	Structural	55:64	arg1	Characterisation					66:81	Biochemical and Structural Characterisation	39:81	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.	0:138	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.					
32050450	7	4	theme	oligomeric	1404:1413	arg1	Exg-D					1373:1377	Exg-D	1373:1377	Exg-D	1373:1377	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	7	4	theme	oligomeric	1404:1413	arg1	hydrolase					1425:1433	a novel and promising oligomeric glycoside hydrolase	1382:1433	a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit	1382:1519	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	2	5	dep	protein	524:530	arg1	respectively					533:544	respectively	533:544	respectively	533:544	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	3	6	dep	60	695:696	arg1	to					692:693	to	692:693	to	692:693	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	5	7	theme	several	1129:1135	arg1	alcohols					1145:1152	several primary alcohols	1129:1152	several primary alcohols	1129:1152	Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides.					
32050450	5	7	theme	several	1129:1135	arg1	cellobioside					1092:1103	p-nitrophenyl cellobioside	1078:1103	p-nitrophenyl cellobioside	1078:1103	Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides.					
32050450	3	8	theme	higher	748:753	arg1	affinity					755:762	a higher affinity	746:762	a higher affinity towards β-glucan	746:779	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	1	9	theme	alkyl	346:350	arg1	synthesis					362:370	alkyl glycoside synthesis	346:370	alkyl glycoside synthesis	346:370	An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.					
32050450	4	10	theme	%	995:995	arg1	coils					1030:1034	11% α-helices, 36% β-strands and 53% coils	993:1034	11% α-helices, 36% β-strands and 53% coils	993:1034	The circular dichroism profile of Exg-D showed that its secondary structure consisted of 11% α-helices, 36% β-strands and 53% coils.					
32050450	4	11	theme	α-helices	997:1005	arg1	%					995:995	11% α-helices	993:1005	11% α-helices	993:1005	The circular dichroism profile of Exg-D showed that its secondary structure consisted of 11% α-helices, 36% β-strands and 53% coils.					
32050450	1	12	theme	glycoside	352:360	arg1	synthesis					362:370	alkyl glycoside synthesis	346:370	alkyl glycoside synthesis	346:370	An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.					
32050450	4	13	theme	circular	908:915	arg1	profile					927:933	The circular dichroism profile	904:933	The circular dichroism profile of Exg-D	904:942	The circular dichroism profile of Exg-D showed that its secondary structure consisted of 11% α-helices, 36% β-strands and 53% coils.					
32050450	3	14	contain	had	857:859	arg1	lichenin					842:849	lichenin	842:849	lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1	842:901	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	3	14	contain	had	857:859	arg2	Km					863:864	a Km	861:864	a Km of 21.5 mg/mL	861:878	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	3	14	contain	had	857:859	arg2	kcat					886:889	a kcat	884:889	a kcat of 70.0 s-1	884:901	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	2	15	theme	highest	462:468	arg1	activity					470:477	highest activity	462:477	highest activity	462:477	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	6	16	theme	chromatography-mass	1313:1331	arg1	LC-MS					1347:1351	LC-MS	1347:1351	LC-MS	1347:1351	These products were identified and quantified via thin-layer chromatography (TLC) and liquid chromatography-mass spectrometry (LC-MS).					
32050450	6	16	theme	chromatography-mass	1313:1331	arg1	spectrometry					1333:1344	liquid chromatography-mass spectrometry	1306:1344	liquid chromatography-mass spectrometry (LC-MS)	1306:1352	These products were identified and quantified via thin-layer chromatography (TLC) and liquid chromatography-mass spectrometry (LC-MS).					
32050450	0	17	theme	Novel	2:6	arg1	GH5_38					30:35	GH5_38	30:35	GH5_38	30:35	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.					
32050450	0	17	theme	Novel	2:6	arg1	Exoglucanase					16:27	A Novel Dimeric Exoglucanase	0:27	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.	0:138	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.					
32050450	2	18	dep	lichenin	551:558	arg1	protein					580:586	24.5 and 23.7 U/mg protein	561:586	24.5 and 23.7 U/mg protein	561:586	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	3	19	theme	optimum	676:682	arg1	studies					710:716	a temperature optimum from 40 to 60 °C. Kinetic studies	662:716	a temperature optimum from 40 to 60 °C. Kinetic studies	662:716	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	2	20	theme	purified	377:384	arg1	Exg-D					386:390	The purified Exg-D	373:390	The purified Exg-D	373:390	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	2	21	dep	protein	580:586	arg1	respectively					589:600	respectively	589:600	respectively	589:600	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	7	22	theme	novel	1384:1388	arg1	Exg-D					1373:1377	Exg-D	1373:1377	Exg-D	1373:1377	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	7	22	theme	novel	1384:1388	arg1	hydrolase					1425:1433	a novel and promising oligomeric glycoside hydrolase	1382:1433	a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit	1382:1519	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	1	23	theme	glycoside	173:181	arg1	family					193:198	the glycoside hydrolase family	169:198	the glycoside hydrolase family 5 subfamily 38 (GH5_38)	169:222	An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.					
32050450	6	24	theme	liquid	1306:1311	arg1	LC-MS					1347:1351	LC-MS	1347:1351	LC-MS	1347:1351	These products were identified and quantified via thin-layer chromatography (TLC) and liquid chromatography-mass spectrometry (LC-MS).					
32050450	6	24	theme	liquid	1306:1311	arg1	spectrometry					1333:1344	liquid chromatography-mass spectrometry	1306:1344	liquid chromatography-mass spectrometry (LC-MS)	1306:1352	These products were identified and quantified via thin-layer chromatography (TLC) and liquid chromatography-mass spectrometry (LC-MS).					
32050450	0	25	theme	Cellobioside	116:127	arg1	Synthesis					129:137	Alkyl Cellobioside Synthesis	110:137	Alkyl Cellobioside Synthesis	110:137	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.					
32050450	3	26	theme	s-1	899:901	arg1	kcat					886:889	a kcat	884:889	a kcat of 70.0 s-1	884:901	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	3	26	theme	s-1	899:901	arg1	Km					863:864	a Km	861:864	a Km of 21.5 mg/mL	861:878	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	4	27	theme	secondary	960:968	arg1	structure					970:978	its secondary structure	956:978	its secondary structure	956:978	The circular dichroism profile of Exg-D showed that its secondary structure consisted of 11% α-helices, 36% β-strands and 53% coils.					
32050450	1	28	theme	hydrolase	183:191	arg1	family					193:198	the glycoside hydrolase family	169:198	the glycoside hydrolase family 5 subfamily 38 (GH5_38)	169:222	An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.					
32050450	2	29	theme	monomeric	420:428	arg1	forms					430:434	both dimeric and monomeric forms	403:434	both dimeric and monomeric forms in solution	403:446	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	0	30	theme	Alkyl	110:114	arg1	Synthesis					129:137	Alkyl Cellobioside Synthesis	110:137	Alkyl Cellobioside Synthesis	110:137	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.					
32050450	7	31	theme	glycoside	1415:1423	arg1	Exg-D					1373:1377	Exg-D	1373:1377	Exg-D	1373:1377	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	7	31	theme	glycoside	1415:1423	arg1	hydrolase					1425:1433	a novel and promising oligomeric glycoside hydrolase	1382:1433	a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit	1382:1519	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	3	32	theme	s-1	825:827	arg1	Km					789:790	a Km	787:790	a Km of 7.9 mg/mL	787:803	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	3	32	theme	s-1	825:827	arg1	kcat					811:814	a kcat	809:814	a kcat of 117.2 s-1	809:827	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	2	33	theme	dimeric	408:414	arg1	forms					430:434	both dimeric and monomeric forms	403:434	both dimeric and monomeric forms in solution	403:446	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	1	34	from	application	331:341	arg1	synthesis					362:370	alkyl glycoside synthesis	346:370	alkyl glycoside synthesis	346:370	An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.					
32050450	3	35	dep	7	656:656	arg1	to					653:654	to	653:654	to	653:654	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	0	36	theme	Dimeric	8:14	arg1	GH5_38					30:35	GH5_38	30:35	GH5_38	30:35	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.					
32050450	0	36	theme	Dimeric	8:14	arg1	Exoglucanase					16:27	A Novel Dimeric Exoglucanase	0:27	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.	0:138	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.					
32050450	7	37	theme	promising	1394:1402	arg1	Exg-D					1373:1377	Exg-D	1373:1377	Exg-D	1373:1377	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	7	37	theme	promising	1394:1402	arg1	hydrolase					1425:1433	a novel and promising oligomeric glycoside hydrolase	1382:1433	a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit	1382:1519	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	4	38	theme	dichroism	917:925	arg1	profile					927:933	The circular dichroism profile	904:933	The circular dichroism profile of Exg-D	904:942	The circular dichroism profile of Exg-D showed that its secondary structure consisted of 11% α-helices, 36% β-strands and 53% coils.					
32050450	4	39	theme	%	1028:1028	arg1	coils					1030:1034	11% α-helices, 36% β-strands and 53% coils	993:1034	11% α-helices, 36% β-strands and 53% coils	993:1034	The circular dichroism profile of Exg-D showed that its secondary structure consisted of 11% α-helices, 36% β-strands and 53% coils.					
32050450	3	40	theme	Kinetic	702:708	arg1	studies					710:716	a temperature optimum from 40 to 60 °C. Kinetic studies	662:716	a temperature optimum from 40 to 60 °C. Kinetic studies	662:716	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	7	41	theme	monosaccharide	1501:1514	arg1	unit					1516:1519	more than one monosaccharide unit	1487:1519	more than one monosaccharide unit	1487:1519	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	0	42	theme	Biochemical	39:49	arg1	Characterisation					66:81	Biochemical and Structural Characterisation	39:81	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.	0:138	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.					
32050450	5	43	theme	glycosyl	1110:1117	arg1	cellobioside					1092:1103	p-nitrophenyl cellobioside	1078:1103	p-nitrophenyl cellobioside	1078:1103	Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides.					
32050450	5	43	theme	glycosyl	1110:1117	arg1	donor					1119:1123	a glycosyl donor	1108:1123	a glycosyl donor	1108:1123	Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides.					
32050450	3	44	from	7	656:656	arg1	range					638:642	a broad optimum pH range	619:642	a broad optimum pH range from 5.5 to 7	619:656	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	1	45	theme	molecular	307:315	arg1	level					317:321	a molecular level	305:321	a molecular level for its application in alkyl glycoside synthesis	305:370	An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.					
32050450	3	46	theme	pH	635:636	arg1	range					638:642	a broad optimum pH range	619:642	a broad optimum pH range from 5.5 to 7	619:656	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	5	47	theme	primary	1137:1143	arg1	alcohols					1145:1152	several primary alcohols	1129:1152	several primary alcohols	1129:1152	Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides.					
32050450	5	47	theme	primary	1137:1143	arg1	cellobioside					1092:1103	p-nitrophenyl cellobioside	1078:1103	p-nitrophenyl cellobioside	1078:1103	Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides.					
32050450	3	48	contain	had	742:744	arg2	affinity					755:762	a higher affinity	746:762	a higher affinity towards β-glucan	746:779	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	3	48	contain	had	742:744	arg1	Exg-D					736:740	Exg-D	736:740	Exg-D	736:740	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	2	49	dep	β-glucan	495:502	arg1	protein					524:530	88.0 and 86.7 U/mg protein	505:530	88.0 and 86.7 U/mg protein	505:530	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	0	50	from	Application	95:105	arg1	Synthesis					129:137	Alkyl Cellobioside Synthesis	110:137	Alkyl Cellobioside Synthesis	110:137	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.					
32050450	0	51	dep	Exoglucanase	16:27	arg1	Characterisation					66:81	Biochemical and Structural Characterisation	39:81	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.	0:138	A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis.					
32050450	2	52	link	mixed-linked	482:493	arg1	β-glucan					495:502	mixed-linked β-glucan	482:502	mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively)	482:545	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	4	53	theme	Exg-D	938:942	arg1	profile					927:933	The circular dichroism profile	904:933	The circular dichroism profile of Exg-D	904:942	The circular dichroism profile of Exg-D showed that its secondary structure consisted of 11% α-helices, 36% β-strands and 53% coils.					
32050450	5	54	theme	p-nitrophenyl	1078:1090	arg1	cellobioside					1092:1103	p-nitrophenyl cellobioside	1078:1103	p-nitrophenyl cellobioside	1078:1103	Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides.					
32050450	5	54	theme	p-nitrophenyl	1078:1090	arg1	donor					1119:1123	a glycosyl donor	1108:1123	a glycosyl donor	1108:1123	Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides.					
32050450	5	54	theme	p-nitrophenyl	1078:1090	arg1	alcohols					1145:1152	several primary alcohols	1129:1152	several primary alcohols	1129:1152	Exg-D performed transglycosylation using p-nitrophenyl cellobioside as a glycosyl donor and several primary alcohols as acceptors to produce methyl-, ethyl- and propyl-cellobiosides.					
32050450	1	55	from	family	193:198	arg1	exoglucanase					143:154	An exoglucanase	140:154	An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38)	140:222	An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.					
32050450	1	55	from	family	193:198	arg1	Exg-D					157:161	Exg-D	157:161	Exg-D	157:161	An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.					
32050450	3	56	theme	broad	621:625	arg1	range					638:642	a broad optimum pH range	619:642	a broad optimum pH range from 5.5 to 7	619:656	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	4	57	theme	%	1010:1010	arg1	coils					1030:1034	11% α-helices, 36% β-strands and 53% coils	993:1034	11% α-helices, 36% β-strands and 53% coils	993:1034	The circular dichroism profile of Exg-D showed that its secondary structure consisted of 11% α-helices, 36% β-strands and 53% coils.					
32050450	6	58	theme	thin-layer	1270:1279	arg1	TLC					1297:1299	TLC	1297:1299	TLC	1297:1299	These products were identified and quantified via thin-layer chromatography (TLC) and liquid chromatography-mass spectrometry (LC-MS).					
32050450	6	58	theme	thin-layer	1270:1279	arg1	chromatography					1281:1294	thin-layer chromatography	1270:1294	thin-layer chromatography (TLC)	1270:1300	These products were identified and quantified via thin-layer chromatography (TLC) and liquid chromatography-mass spectrometry (LC-MS).					
32050450	3	59	theme	optimum	627:633	arg1	range					638:642	a broad optimum pH range	619:642	a broad optimum pH range from 5.5 to 7	619:656	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	3	60	theme	mg/mL	874:878	arg1	kcat					886:889	a kcat	884:889	a kcat of 70.0 s-1	884:901	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	3	60	theme	mg/mL	874:878	arg1	Km					863:864	a Km	861:864	a Km of 21.5 mg/mL	861:878	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	2	61	theme	mixed-linked	482:493	arg1	β-glucan					495:502	mixed-linked β-glucan	482:502	mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively)	482:545	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	2	62	from	forms	430:434	arg1	solution					439:446	solution	439:446	solution	439:446	The purified Exg-D existed in both dimeric and monomeric forms in solution, which showed highest activity on mixed-linked β-glucan (88.0 and 86.7 U/mg protein, respectively) and lichenin (24.5 and 23.7 U/mg protein, respectively).					
32050450	1	63	dep	family	193:198	arg1	subfamily					202:210	5 subfamily 38 (GH5_38)	200:222	the glycoside hydrolase family 5 subfamily 38 (GH5_38)	169:222	An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.					
32050450	7	64	with	glycosides	1471:1480	arg1	unit					1516:1519	more than one monosaccharide unit	1487:1519	more than one monosaccharide unit	1487:1519	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	7	65	theme	glycosides	1471:1480	arg1	synthesis					1452:1460	the one-step synthesis	1439:1460	the one-step synthesis of alkyl glycosides with more than one monosaccharide unit	1439:1519	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
32050450	3	66	theme	mg/mL	799:803	arg1	Km					789:790	a Km	787:790	a Km of 7.9 mg/mL	787:803	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	3	66	theme	mg/mL	799:803	arg1	kcat					811:814	a kcat	809:814	a kcat of 117.2 s-1	809:827	They displayed a broad optimum pH range from 5.5 to 7 and a temperature optimum from 40 to 60 °C. Kinetic studies demonstrated that Exg-D had a higher affinity towards β-glucan, with a Km of 7.9 mg/mL and a kcat of 117.2 s-1, compared to lichenin which had a Km of 21.5 mg/mL and a kcat of 70.0 s-1.					
32050450	7	67	theme	alkyl	1465:1469	arg1	glycosides					1471:1480	alkyl glycosides	1465:1480	alkyl glycosides with more than one monosaccharide unit	1465:1519	We concluded that Exg-D is a novel and promising oligomeric glycoside hydrolase for the one-step synthesis of alkyl glycosides with more than one monosaccharide unit.					
34051603	1	0	theme	part	162:165	arg1	composition					209:219	the monosaccharide composition	190:219	the monosaccharide composition	190:219	Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1.					
34051603	2	1	theme	polymer	343:349	arg1	chain					351:355	a main polymer chain	336:355	a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments	336:553	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	2	2	from	-β-Galp-	533:540	arg1	position					416:423	position	416:423	position of C-3	416:430	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	1	3	attach	isolated	137:144	arg1	part					162:165	the aerial part	151:165	the aerial part	151:165	Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1.					
34051603	1	3	attach	isolated	137:144	arg2	arabinogalactan					88:102	Acidic arabinogalactan	81:102	Acidic arabinogalactan with molecular weight 36 kDa	81:131	Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1.					
34051603	4	4	theme	arabinogalactan	677:691	arg1	effect					667:672	The effect	663:672	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli	663:787	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.					
34051603	2	5	from	residues	441:448	arg1	position					416:423	position	416:423	position of C-3	416:430	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	4	6	theme	associative	713:723	arg1	culture					725:731	an associative culture	710:731	an associative culture of bifidobacteria and some mono-strains of lactobacilli	710:787	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.					
34051603	2	7	theme	β-galactopyranose	379:395	arg1	residues					397:404	(1 → 6) β-galactopyranose residues	371:404	(1 → 6) β-galactopyranose residues	371:404	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	3	8	theme	C-2	658:660	arg1	position					649:656	position C-2	649:660	position C-2	649:660	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	1	9	theme	Acidic	81:86	arg1	arabinogalactan					88:102	Acidic arabinogalactan	81:102	Acidic arabinogalactan with molecular weight 36 kDa	81:131	Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1.					
34051603	1	10	theme	kuhistаnica	177:187	arg1	part					162:165	the aerial part	151:165	the aerial part	151:165	Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1.					
34051603	2	11	theme	spectral	310:317	arg1	methods					319:325	The chemical and spectral methods	293:325	The chemical and spectral methods	293:325	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	2	12	theme	1 → 6	372:376	arg1	residues					397:404	(1 → 6) β-galactopyranose residues	371:404	(1 → 6) β-galactopyranose residues	371:404	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	2	13	theme	C-3	428:430	arg1	position					416:423	position	416:423	position of C-3	416:430	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	1	14	with	arabinogalactan	88:102	arg1	weight					119:124	molecular weight 36 kDa	109:131	molecular weight 36 kDa	109:131	Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1.					
34051603	1	15	theme	molecular	109:117	arg1	weight					119:124	molecular weight 36 kDa	109:131	molecular weight 36 kDa	109:131	Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1.					
34051603	1	16	theme	monosaccharide	194:207	arg1	composition					209:219	the monosaccharide composition	190:219	the monosaccharide composition	190:219	Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1.					
34051603	1	17	theme	3.6:1	286:290	arg1	ratio					277:281	a ratio	275:281	a ratio of 3.6:1	275:290	Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1.					
34051603	3	18	theme	chain	594:598	arg1	part					577:580	a small part	569:580	a small part of the main chain	569:598	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	2	19	theme	α-arabinofuranose	453:469	arg1	residues					441:448	the residues	437:448	the residues of α-arabinofuranose and its 1,5-linked oligomers	437:498	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	2	19	theme	α-arabinofuranose	453:469	arg1	oligomers					490:498	its 1,5-linked oligomers	475:498	its 1,5-linked oligomers	475:498	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	2	19	theme	α-arabinofuranose	453:469	arg1	-β-Galp-					533:540	β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments	512:553	β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments	512:553	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	2	19	theme	α-arabinofuranose	453:469	arg1	α-arabinofuranose					453:469	α-arabinofuranose	453:469	α-arabinofuranose	453:469	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	4	20	theme	mono-strains	760:771	arg1	culture					725:731	an associative culture	710:731	an associative culture of bifidobacteria and some mono-strains of lactobacilli	710:787	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.					
34051603	3	21	contain	carries	600:606	arg2	β-Galp-					635:641	β-Galp-	635:641	β-Galp-	635:641	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	3	21	contain	carries	600:606	arg2	residues					623:630	monosaccharide residues	608:630	monosaccharide residues of β-Galp-	608:641	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	3	21	contain	carries	600:606	arg1	part					577:580	a small part	569:580	a small part of the main chain	569:598	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	3	21	contain	carries	600:606	arg3	addition					559:566	addition	559:566	addition	559:566	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	3	21	contain	carries	600:606	arg3	position					649:656	position C-2	649:660	position C-2	649:660	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	2	22	theme	β-GlcpA-4-OMe-	512:525	arg1	-β-Galp-					533:540	β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments	512:553	β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments	512:553	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	0	23	from	Kuhistаnica	68:78	arg1	prebiotic					18:26	prebiotic	18:26	prebiotic	18:26	The structure and prebiotic activity of arabinogalactan from Ferula Kuhistаnica.					
34051603	0	23	from	Kuhistаnica	68:78	arg1	structure					4:12	structure	4:12	structure	4:12	The structure and prebiotic activity of arabinogalactan from Ferula Kuhistаnica.					
34051603	2	24	dep	-β-Galp-	533:540	arg1	fragments					545:553	1→ fragments	542:553	β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments	512:553	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	4	25	theme	lactobacilli	776:787	arg1	bifidobacteria					736:749	bifidobacteria	736:749	bifidobacteria	736:749	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.					
34051603	4	25	theme	lactobacilli	776:787	arg1	mono-strains					760:771	some mono-strains	755:771	some mono-strains of lactobacilli	755:787	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.					
34051603	2	26	theme	main	338:341	arg1	chain					351:355	a main polymer chain	336:355	a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments	336:553	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	0	27	theme	arabinogalactan	40:54	arg1	prebiotic					18:26	prebiotic	18:26	prebiotic	18:26	The structure and prebiotic activity of arabinogalactan from Ferula Kuhistаnica.					
34051603	0	27	theme	arabinogalactan	40:54	arg1	structure					4:12	structure	4:12	structure	4:12	The structure and prebiotic activity of arabinogalactan from Ferula Kuhistаnica.					
34051603	2	28	theme	1→	542:543	arg1	fragments					545:553	1→ fragments	542:553	β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments	512:553	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	3	29	theme	main	589:592	arg1	chain					594:598	the main chain	585:598	the main chain	585:598	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	4	30	from	effect	667:672	arg1	growth					700:705	the growth	696:705	the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli	696:787	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.					
34051603	4	31	theme	bifidobacteria	736:749	arg1	culture					725:731	an associative culture	710:731	an associative culture of bifidobacteria and some mono-strains of lactobacilli	710:787	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.					
34051603	4	32	theme	prebiotic	850:858	arg1	activity					860:867	more effective prebiotic activity	835:867	more effective prebiotic activity	835:867	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.					
34051603	3	33	theme	β-Galp-	635:641	arg1	residues					623:630	monosaccharide residues	608:630	monosaccharide residues of β-Galp-	608:641	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	3	33	theme	β-Galp-	635:641	arg1	β-Galp-					635:641	β-Galp-	635:641	β-Galp-	635:641	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	2	34	link	1,5-linked	479:488	arg1	oligomers					490:498	its 1,5-linked oligomers	475:498	its 1,5-linked oligomers	475:498	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	2	35	theme	chemical	297:304	arg1	methods					319:325	The chemical and spectral methods	293:325	The chemical and spectral methods	293:325	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	4	36	theme	effective	840:848	arg1	activity					860:867	more effective prebiotic activity	835:867	more effective prebiotic activity	835:867	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.					
34051603	3	37	theme	monosaccharide	608:621	arg1	residues					623:630	monosaccharide residues	608:630	monosaccharide residues of β-Galp-	608:641	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	3	37	theme	monosaccharide	608:621	arg1	β-Galp-					635:641	β-Galp-	635:641	β-Galp-	635:641	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	4	38	theme	culture	725:731	arg1	growth					700:705	the growth	696:705	the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli	696:787	The effect of arabinogalactan on the growth of an associative culture of bifidobacteria and some mono-strains of lactobacilli was studied and it was shown that it exhibits more effective prebiotic activity.					
34051603	2	39	theme	oligomers	490:498	arg1	residues					441:448	the residues	437:448	the residues of α-arabinofuranose and its 1,5-linked oligomers	437:498	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	2	39	theme	oligomers	490:498	arg1	oligomers					490:498	its 1,5-linked oligomers	475:498	its 1,5-linked oligomers	475:498	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	2	39	theme	oligomers	490:498	arg1	-β-Galp-					533:540	β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments	512:553	β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments	512:553	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	2	39	theme	oligomers	490:498	arg1	α-arabinofuranose					453:469	α-arabinofuranose	453:469	α-arabinofuranose	453:469	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	0	40	dep	structure	4:12	arg1	The					0:2	The	0:2	The	0:2	The structure and prebiotic activity of arabinogalactan from Ferula Kuhistаnica.					
34051603	0	40	dep	structure	4:12	arg1	activity					28:35	activity	28:35	activity	28:35	The structure and prebiotic activity of arabinogalactan from Ferula Kuhistаnica.					
34051603	2	41	theme	1,5-linked	479:488	arg1	oligomers					490:498	its 1,5-linked oligomers	475:498	its 1,5-linked oligomers	475:498	The chemical and spectral methods revealed a main polymer chain consisting of (1 → 6) β-galactopyranose residues, where in position of C-3 were the residues of α-arabinofuranose and its 1,5-linked oligomers, as well as β-GlcpA-4-OMe-(1 → 6)-β-Galp-(1→ fragments.					
34051603	3	42	theme	small	571:575	arg1	part					577:580	a small part	569:580	a small part of the main chain	569:598	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
34051603	1	43	theme	aerial	155:160	arg1	part					162:165	the aerial part	151:165	the aerial part	151:165	Acidic arabinogalactan with molecular weight 36 kDa was isolated from the aerial part of Ferula kuhistаnica, the monosaccharide composition of which is represented by galactose and arabinose in a ratio of 3.6:1.					
34051603	3	44	dep	carries	600:606	arg1	1→					643:644	1→	643:644	1→	643:644	In addition, a small part of the main chain carries monosaccharide residues of β-Galp-(1→ in position C-2.					
33284837	8	0	theme	network	1252:1258	arg1	structure					1260:1268	a more heterogeneous connected network structure	1221:1268	a more heterogeneous connected network structure	1221:1268	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	6	1	theme	hub	920:922	arg1	nodes					924:928	the hub nodes	916:928	the hub nodes	916:928	This suggests that the hub nodes are impacted by the immune response and the resulting loss of fitness is compensated by mutations at the spoke positions.					
33284837	4	2	theme	subtype	692:698	arg1	B					700:700	156 subtype B	688:700	156 subtype B	688:700	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	4	3	theme	research	565:572	arg1	methods					593:599	research and bioinformatics methods	565:599	methods	593:599	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	8	4	theme	heterogeneous	1228:1240	arg1	structure					1260:1268	a more heterogeneous connected network structure	1221:1268	a more heterogeneous connected network structure	1221:1268	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	3	5	theme	essential	486:494	arg1	pathways					496:503	the essential pathways	482:503	the essential pathways it takes to maintain infection	482:534	Determining this pathway of coevolving mutations can assist in understanding what is different with the founder virus and the essential pathways it takes to maintain infection.					
33284837	10	6	theme	immune	1657:1662	arg1	response					1664:1671	the immune response	1653:1671	the immune response	1653:1671	Our study provides insights into the mutational pathways HIV takes to evade the immune response, and presents features more likely to establish founder infection, valuable for effective vaccine design.					
33284837	10	7	theme	vaccine	1763:1769	arg1	design					1771:1776	effective vaccine design	1753:1776	effective vaccine design	1753:1776	Our study provides insights into the mutational pathways HIV takes to evade the immune response, and presents features more likely to establish founder infection, valuable for effective vaccine design.					
33284837	10	8	theme	founder	1721:1727	arg1	infection					1729:1737	founder infection	1721:1737	founder infection	1721:1737	Our study provides insights into the mutational pathways HIV takes to evade the immune response, and presents features more likely to establish founder infection, valuable for effective vaccine design.					
33284837	4	9	dep	B	700:700	arg1	sequences					737:745	envelope (gp160) sequences	720:745	envelope (gp160) sequences	720:745	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	4	10	theme	bioinformatics	578:591	arg1	methods					593:599	research and bioinformatics methods	565:599	methods	593:599	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	4	11	theme	envelope	720:727	arg1	sequences					737:745	envelope (gp160) sequences	720:745	envelope (gp160) sequences	720:745	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	1	12	theme	Human	64:68	arg1	HIV					94:96	HIV	94:96	HIV	94:96	Human immunodeficiency virus (HIV) is subject to immune selective pressure soon after it establishes infection at the founder stage.					
33284837	1	12	theme	Human	64:68	arg1	virus					87:91	Human immunodeficiency virus	64:91	Human immunodeficiency virus (HIV)	64:97	Human immunodeficiency virus (HIV) is subject to immune selective pressure soon after it establishes infection at the founder stage.					
33284837	2	13	theme	infection	262:270	arg1	stage					253:257	the founder to chronic stage	230:257	the founder to chronic stage of infection	230:270	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	3	14	theme	founder	464:470	arg1	virus					472:476	the founder virus	460:476	the founder virus	460:476	Determining this pathway of coevolving mutations can assist in understanding what is different with the founder virus and the essential pathways it takes to maintain infection.					
33284837	7	15	theme	chronic	1074:1080	arg1	network					1084:1090	the chronic C network	1070:1090	the chronic C network	1070:1090	The major hubs in the chronic C network occur at positions 12, 137 (within the N136 glycan), and 822, and at position 306 for subtype B.					
33284837	1	16	theme	immunodeficiency	70:85	arg1	HIV					94:96	HIV	94:96	HIV	94:96	Human immunodeficiency virus (HIV) is subject to immune selective pressure soon after it establishes infection at the founder stage.					
33284837	1	16	theme	immunodeficiency	70:85	arg1	virus					87:91	Human immunodeficiency virus	64:91	Human immunodeficiency virus (HIV)	64:97	Human immunodeficiency virus (HIV) is subject to immune selective pressure soon after it establishes infection at the founder stage.					
33284837	5	17	theme	structured	863:872	arg1	networks					887:894	the less structured transmission networks	854:894	the less structured transmission networks	854:894	The chronic networks for both subtypes revealed strikingly different hub-and-spoke topologies compared to the less structured transmission networks.					
33284837	4	18	theme	founder	657:663	arg1	stages					677:682	founder and chronic stages	657:682	founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences	657:745	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	6	19	theme	resulting	974:982	arg1	loss					984:987	the resulting loss	970:987	the resulting loss of fitness	970:998	This suggests that the hub nodes are impacted by the immune response and the resulting loss of fitness is compensated by mutations at the spoke positions.					
33284837	5	20	theme	chronic	752:758	arg1	networks					760:767	The chronic networks	748:767	The chronic networks for both subtypes	748:785	The chronic networks for both subtypes revealed strikingly different hub-and-spoke topologies compared to the less structured transmission networks.					
33284837	2	21	from	embedded	328:335	arg1	sequences					349:357	envelope sequences	340:357	envelope sequences	340:357	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	8	22	theme	founder	1285:1291	arg1	subnetworks					1295:1305	founder B subnetworks	1285:1305	founder B subnetworks around positions 640 and 837	1285:1334	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	7	23	from	hubs	1062:1065	arg1	network					1084:1090	the chronic C network	1070:1090	the chronic C network	1070:1090	The major hubs in the chronic C network occur at positions 12, 137 (within the N136 glycan), and 822, and at position 306 for subtype B.					
33284837	10	24	theme	mutational	1614:1623	arg1	pathways					1625:1632	the mutational pathways	1610:1632	the mutational pathways HIV takes to evade the immune response	1610:1671	Our study provides insights into the mutational pathways HIV takes to evade the immune response, and presents features more likely to establish founder infection, valuable for effective vaccine design.					
33284837	7	25	theme	N136	1131:1134	arg1	glycan					1136:1141	the N136 glycan	1127:1141	the N136 glycan	1127:1141	The major hubs in the chronic C network occur at positions 12, 137 (within the N136 glycan), and 822, and at position 306 for subtype B.					
33284837	9	26	theme	chronic	1477:1483	arg1	B					1493:1493	chronic subtype B	1477:1493	chronic subtype B	1477:1493	Finally, we observed a differential effect of glycosylation between founder and chronic subtype B where the latter had mutational pathways significantly driven by N-glycosylation.					
33284837	10	27	theme	valuable	1740:1747	arg1	infection					1729:1737	founder infection	1721:1737	founder infection	1721:1737	Our study provides insights into the mutational pathways HIV takes to evade the immune response, and presents features more likely to establish founder infection, valuable for effective vaccine design.					
33284837	4	28	contain	have	540:543	arg1	We					537:538	We	537:538	We	537:538	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	4	28	contain	have	540:543	arg2	methods					593:599	research and bioinformatics methods	565:599	methods	593:599	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	4	28	contain	have	540:543	arg2	operations					554:563	combined operations	545:563	combined operations	545:563	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	4	29	theme	chronic	669:675	arg1	stages					677:682	founder and chronic stages	657:682	founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences	657:745	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	0	30	theme	Mutational	0:9	arg1	networks					11:18	Mutational networks	0:18	Mutational networks of escape	0:28	Mutational networks of escape from transmitted HIV-1 infection.					
33284837	6	31	from	positions	1041:1049	arg1	mutations					1018:1026	mutations	1018:1026	mutations at the spoke positions	1018:1049	This suggests that the hub nodes are impacted by the immune response and the resulting loss of fitness is compensated by mutations at the spoke positions.					
33284837	1	32	dep	it	150:151	arg1	establishes					153:163	establishes	153:163	establishes infection at the founder stage	153:194	Human immunodeficiency virus (HIV) is subject to immune selective pressure soon after it establishes infection at the founder stage.					
33284837	6	33	theme	fitness	992:998	arg1	loss					984:987	the resulting loss	970:987	the resulting loss of fitness	970:998	This suggests that the hub nodes are impacted by the immune response and the resulting loss of fitness is compensated by mutations at the spoke positions.					
33284837	5	34	theme	transmission	874:885	arg1	networks					887:894	the less structured transmission networks	854:894	the less structured transmission networks	854:894	The chronic networks for both subtypes revealed strikingly different hub-and-spoke topologies compared to the less structured transmission networks.					
33284837	0	35	theme	escape	23:28	arg1	networks					11:18	Mutational networks	0:18	Mutational networks of escape	0:28	Mutational networks of escape from transmitted HIV-1 infection.					
33284837	8	36	theme	binding	1380:1386	arg1	domains					1388:1394	CD4 and coreceptor binding domains	1361:1394	CD4 and coreceptor binding domains	1361:1394	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	7	37	theme	major	1056:1060	arg1	hubs					1062:1065	The major hubs	1052:1065	The major hubs in the chronic C network	1052:1090	The major hubs in the chronic C network occur at positions 12, 137 (within the N136 glycan), and 822, and at position 306 for subtype B.					
33284837	10	38	theme	effective	1753:1761	arg1	design					1771:1776	effective vaccine design	1753:1776	effective vaccine design	1753:1776	Our study provides insights into the mutational pathways HIV takes to evade the immune response, and presents features more likely to establish founder infection, valuable for effective vaccine design.					
33284837	3	39	dep	pathways	496:503	arg1	takes					508:512	takes	508:512	takes to maintain infection	508:534	Determining this pathway of coevolving mutations can assist in understanding what is different with the founder virus and the essential pathways it takes to maintain infection.					
33284837	1	40	theme	founder	182:188	arg1	stage					190:194	the founder stage	178:194	the founder stage	178:194	Human immunodeficiency virus (HIV) is subject to immune selective pressure soon after it establishes infection at the founder stage.					
33284837	5	41	theme	different	807:815	arg1	topologies					831:840	strikingly different hub-and-spoke topologies	796:840	strikingly different hub-and-spoke topologies	796:840	The chronic networks for both subtypes revealed strikingly different hub-and-spoke topologies compared to the less structured transmission networks.					
33284837	4	42	theme	combined	545:552	arg1	operations					554:563	combined operations	545:563	combined operations	545:563	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	8	43	theme	B	1293:1293	arg1	subnetworks					1295:1305	founder B subnetworks	1285:1305	founder B subnetworks around positions 640 and 837	1285:1334	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	2	44	theme	immune	273:278	arg1	pressure					280:287	immune pressure	273:287	immune pressure	273:287	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	9	45	theme	subtype	1485:1491	arg1	B					1493:1493	chronic subtype B	1477:1493	chronic subtype B	1477:1493	Finally, we observed a differential effect of glycosylation between founder and chronic subtype B where the latter had mutational pathways significantly driven by N-glycosylation.					
33284837	6	46	theme	spoke	1035:1039	arg1	positions					1041:1049	the spoke positions	1031:1049	the spoke positions	1031:1049	This suggests that the hub nodes are impacted by the immune response and the resulting loss of fitness is compensated by mutations at the spoke positions.					
33284837	3	47	theme	coevolving	388:397	arg1	mutations					399:407	coevolving mutations	388:407	coevolving mutations	388:407	Determining this pathway of coevolving mutations can assist in understanding what is different with the founder virus and the essential pathways it takes to maintain infection.					
33284837	6	48	theme	immune	950:955	arg1	response					957:964	the immune response	946:964	the immune response	946:964	This suggests that the hub nodes are impacted by the immune response and the resulting loss of fitness is compensated by mutations at the spoke positions.					
33284837	3	49	with	different	445:453	arg1	virus					472:476	the founder virus	460:476	the founder virus	460:476	Determining this pathway of coevolving mutations can assist in understanding what is different with the founder virus and the essential pathways it takes to maintain infection.					
33284837	3	49	with	different	445:453	arg1	pathways					496:503	the essential pathways	482:503	the essential pathways it takes to maintain infection	482:534	Determining this pathway of coevolving mutations can assist in understanding what is different with the founder virus and the essential pathways it takes to maintain infection.					
33284837	7	50	theme	subtype	1178:1184	arg1	B					1186:1186	subtype B	1178:1186	subtype B	1178:1186	The major hubs in the chronic C network occur at positions 12, 137 (within the N136 glycan), and 822, and at position 306 for subtype B.					
33284837	8	51	theme	connected	1242:1250	arg1	structure					1260:1268	a more heterogeneous connected network structure	1221:1268	a more heterogeneous connected network structure	1221:1268	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	8	52	theme	founder	1200:1206	arg1	networks					1208:1215	both founder networks	1195:1215	both founder networks	1195:1215	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	3	53	theme	mutations	399:407	arg1	pathway					377:383	this pathway	372:383	this pathway of coevolving mutations	372:407	Determining this pathway of coevolving mutations can assist in understanding what is different with the founder virus and the essential pathways it takes to maintain infection.					
33284837	8	54	contain	contained	1351:1359	arg2	domains					1388:1394	CD4 and coreceptor binding domains	1361:1394	CD4 and coreceptor binding domains	1361:1394	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	8	54	contain	contained	1351:1359	arg1	subnetworks					1295:1305	founder B subnetworks	1285:1305	founder B subnetworks around positions 640 and 837	1285:1334	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	4	55	theme	gp160	730:734	arg1	sequences					737:745	envelope (gp160) sequences	720:745	envelope (gp160) sequences	720:745	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	9	56	contain	had	1512:1514	arg2	pathways					1527:1534	mutational pathways	1516:1534	mutational pathways significantly driven by N-glycosylation	1516:1574	Finally, we observed a differential effect of glycosylation between founder and chronic subtype B where the latter had mutational pathways significantly driven by N-glycosylation.					
33284837	9	56	contain	had	1512:1514	arg1	latter					1505:1510	latter	1505:1510	latter	1505:1510	Finally, we observed a differential effect of glycosylation between founder and chronic subtype B where the latter had mutational pathways significantly driven by N-glycosylation.					
33284837	2	57	theme	individual	203:212	arg1	progresses					214:223	an individual progresses	200:223	an individual progresses from the founder to chronic stage of infection	200:270	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	2	57	theme	individual	203:212	arg1	history					298:304	a history	296:304	a history of mutations that are embedded in envelope sequences	296:357	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	2	58	from	sequences	349:357	arg1	embedded					328:335	embedded	328:335	embedded	328:335	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	0	59	theme	HIV-1	47:51	arg1	infection					53:61	HIV-1 infection	47:61	HIV-1 infection	47:61	Mutational networks of escape from transmitted HIV-1 infection.					
33284837	9	60	theme	glycosylation	1443:1455	arg1	effect					1433:1438	a differential effect	1418:1438	a differential effect of glycosylation between founder and chronic subtype B where the latter had mutational pathways significantly driven by N-glycosylation	1418:1574	Finally, we observed a differential effect of glycosylation between founder and chronic subtype B where the latter had mutational pathways significantly driven by N-glycosylation.					
33284837	7	61	theme	C	1082:1082	arg1	network					1084:1090	the chronic C network	1070:1090	the chronic C network	1070:1090	The major hubs in the chronic C network occur at positions 12, 137 (within the N136 glycan), and 822, and at position 306 for subtype B.					
33284837	2	62	theme	mutations	309:317	arg1	progresses					214:223	an individual progresses	200:223	an individual progresses from the founder to chronic stage of infection	200:270	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	2	62	theme	mutations	309:317	arg1	history					298:304	a history	296:304	a history of mutations that are embedded in envelope sequences	296:357	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	8	63	theme	coreceptor	1369:1378	arg1	domains					1388:1394	CD4 and coreceptor binding domains	1361:1394	CD4 and coreceptor binding domains	1361:1394	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	10	64	dep	pathways	1625:1632	arg1	takes					1638:1642	takes	1638:1642	takes to evade the immune response	1638:1671	Our study provides insights into the mutational pathways HIV takes to evade the immune response, and presents features more likely to establish founder infection, valuable for effective vaccine design.					
33284837	2	65	theme	chronic	245:251	arg1	stage					253:257	the founder to chronic stage	230:257	the founder to chronic stage of infection	230:270	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	1	66	theme	immune	113:118	arg1	pressure					130:137	immune selective pressure	113:137	immune selective pressure	113:137	Human immunodeficiency virus (HIV) is subject to immune selective pressure soon after it establishes infection at the founder stage.					
33284837	2	67	from	stage	253:257	arg1	progresses					214:223	an individual progresses	200:223	an individual progresses from the founder to chronic stage of infection	200:270	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	2	67	from	stage	253:257	arg1	history					298:304	a history	296:304	a history of mutations that are embedded in envelope sequences	296:357	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	8	68	contain	had	1217:1219	arg2	structure					1260:1268	a more heterogeneous connected network structure	1221:1268	a more heterogeneous connected network structure	1221:1268	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	8	68	contain	had	1217:1219	arg1	networks					1208:1215	both founder networks	1195:1215	both founder networks	1195:1215	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	8	69	theme	CD4	1361:1363	arg1	domains					1388:1394	CD4 and coreceptor binding domains	1361:1394	CD4 and coreceptor binding domains	1361:1394	While both founder networks had a more heterogeneous connected network structure, interestingly founder B subnetworks around positions 640 and 837 preferentially contained CD4 and coreceptor binding domains.					
33284837	1	70	theme	selective	120:128	arg1	pressure					130:137	immune selective pressure	113:137	immune selective pressure	113:137	Human immunodeficiency virus (HIV) is subject to immune selective pressure soon after it establishes infection at the founder stage.					
33284837	4	71	theme	subtype	710:716	arg1	C					718:718	107 subtype C	706:718	107 subtype C	706:718	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	9	72	theme	differential	1420:1431	arg1	effect					1433:1438	a differential effect	1418:1438	a differential effect of glycosylation between founder and chronic subtype B where the latter had mutational pathways significantly driven by N-glycosylation	1418:1574	Finally, we observed a differential effect of glycosylation between founder and chronic subtype B where the latter had mutational pathways significantly driven by N-glycosylation.					
33284837	4	73	theme	mutations	628:636	arg1	networks					616:623	key networks	612:623	key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences	612:745	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	2	74	theme	founder	234:240	arg1	stage					253:257	the founder to chronic stage	230:257	the founder to chronic stage of infection	230:270	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	5	75	theme	hub-and-spoke	817:829	arg1	topologies					831:840	strikingly different hub-and-spoke topologies	796:840	strikingly different hub-and-spoke topologies	796:840	The chronic networks for both subtypes revealed strikingly different hub-and-spoke topologies compared to the less structured transmission networks.					
33284837	2	76	theme	envelope	340:347	arg1	sequences					349:357	envelope sequences	340:357	envelope sequences	340:357	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
33284837	9	77	theme	mutational	1516:1525	arg1	pathways					1527:1534	mutational pathways	1516:1534	mutational pathways significantly driven by N-glycosylation	1516:1574	Finally, we observed a differential effect of glycosylation between founder and chronic subtype B where the latter had mutational pathways significantly driven by N-glycosylation.					
33284837	4	78	theme	key	612:614	arg1	networks					616:623	key networks	612:623	key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences	612:745	We have combined operations research and bioinformatics methods to extract key networks of mutations that differentiate founder and chronic stages for 156 subtype B and 107 subtype C envelope (gp160) sequences.					
33284837	10	79	theme	likely	1701:1706	arg1	features					1687:1694	features	1687:1694	features more likely to establish founder infection, valuable for effective vaccine design	1687:1776	Our study provides insights into the mutational pathways HIV takes to evade the immune response, and presents features more likely to establish founder infection, valuable for effective vaccine design.					
33284837	7	80	dep	positions	1101:1109	arg1	137					1115:1117	137	1115:1117	137	1115:1117	The major hubs in the chronic C network occur at positions 12, 137 (within the N136 glycan), and 822, and at position 306 for subtype B.					
33284837	7	80	dep	positions	1101:1109	arg1	12					1111:1112	12	1111:1112	12	1111:1112	The major hubs in the chronic C network occur at positions 12, 137 (within the N136 glycan), and 822, and at position 306 for subtype B.					
33284837	7	80	dep	positions	1101:1109	arg1	822					1149:1151	822	1149:1151	822	1149:1151	The major hubs in the chronic C network occur at positions 12, 137 (within the N136 glycan), and 822, and at position 306 for subtype B.					
33284837	2	81	dep	stage	253:257	arg1	to					242:243	to	242:243	to	242:243	As an individual progresses from the founder to chronic stage of infection, immune pressure forces a history of mutations that are embedded in envelope sequences.					
31972195	1	0	theme	water	338:342	arg1	activity					344:351	the water activity	334:351	the water activity from 0.11 to 0.86	334:369	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	1	from	range	325:329	arg1	0.86					366:369	0.86	366:369	0.86	366:369	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	6	2	theme	fruit	1006:1010	arg1	part					986:989	the edible part	975:989	the edible part of the avocado fruit	975:1010	In order to characterize the research material, chemical composition of the edible part of the avocado fruit was determined.					
31972195	5	3	theme	analyzed	886:893	arg1	blends					895:900	all analyzed blends	882:900	all analyzed blends	882:900	It was found that the Peleg model was the most adequate for representation of the sorption data of all analyzed blends.					
31972195	1	4	theme	static	266:271	arg1	method					273:278	gravimetric static method	254:278	gravimetric static method of saturated salt solutions	254:306	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	3	5	theme	square	648:653	arg1	values					655:660	root mean square values	638:660	root mean square values	638:660	The goodness of the fit evaluated on the basis of criteria such as coefficient of the determination and root mean square values.					
31972195	1	6	theme	activity	344:351	arg1	25 °C					311:315	25 °C	311:315	25 °C	311:315	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	6	theme	activity	344:351	arg1	range					325:329	the range	321:329	the range of the water activity from 0.11 to 0.86	321:369	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	6	7	theme	avocado	998:1004	arg1	fruit					1006:1010	the avocado fruit	994:1010	the avocado fruit	994:1010	In order to characterize the research material, chemical composition of the edible part of the avocado fruit was determined.					
31972195	3	8	theme	root	638:641	arg1	values					655:660	root mean square values	638:660	root mean square values	638:660	The goodness of the fit evaluated on the basis of criteria such as coefficient of the determination and root mean square values.					
31972195	4	9	theme	sigmoid	768:774	arg1	shape					776:780	sigmoid shape	768:780	sigmoid shape	768:780	Sorption isotherm of the pure avocado powder was III type while curves plotted for others powders showed sigmoid shape.					
31972195	4	10	theme	pure	688:691	arg1	powder					701:706	the pure avocado powder	684:706	the pure avocado powder	684:706	Sorption isotherm of the pure avocado powder was III type while curves plotted for others powders showed sigmoid shape.					
31972195	4	11	theme	powder	701:706	arg1	isotherm					672:679	Sorption isotherm	663:679	Sorption isotherm of the pure avocado powder	663:706	Sorption isotherm of the pure avocado powder was III type while curves plotted for others powders showed sigmoid shape.					
31972195	7	12	theme	matter	1054:1059	arg1	component					1033:1041	Main component	1028:1041	Main component of the dry matter	1028:1059	Main component of the dry matter was fat.					
31972195	4	13	theme	Sorption	663:670	arg1	isotherm					672:679	Sorption isotherm	663:679	Sorption isotherm of the pure avocado powder	663:706	Sorption isotherm of the pure avocado powder was III type while curves plotted for others powders showed sigmoid shape.					
31972195	1	14	theme	saturated	283:291	arg1	solutions					298:306	saturated salt solutions	283:306	saturated salt solutions	283:306	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	15	contain	containing	166:175	arg2	avocado					177:183	avocado	177:183	avocado	177:183	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	15	contain	containing	166:175	arg1	powders					158:164	the freeze-dried powders	141:164	the freeze-dried powders containing avocado, maltodextrin and inulin	141:208	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	15	contain	containing	166:175	arg2	inulin					203:208	inulin	203:208	inulin	203:208	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	15	contain	containing	166:175	arg2	maltodextrin					186:197	maltodextrin	186:197	maltodextrin	186:197	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	2	16	dep	BET	428:430	arg1	Anderson					475:482	Anderson	475:482	Anderson	475:482	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	16	dep	BET	428:430	arg1	deBoer					488:493	deBoer	488:493	deBoer	488:493	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	17	theme	sorption	411:418	arg1	Henderson					523:531	Henderson	523:531	Henderson	523:531	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	17	theme	sorption	411:418	arg1	Oswin					513:517	Oswin	513:517	Oswin	513:517	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	17	theme	sorption	411:418	arg1	Lewicki					504:510	Lewicki	504:510	Lewicki	504:510	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	17	theme	sorption	411:418	arg1	BET					428:430	BET	428:430	BET	428:430	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	17	theme	sorption	411:418	arg1	models					420:425	sorption models	411:425	sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson	411:531	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	5	18	theme	blends	895:900	arg1	data					874:877	the sorption data	861:877	the sorption data of all analyzed blends	861:900	It was found that the Peleg model was the most adequate for representation of the sorption data of all analyzed blends.					
31972195	1	19	from	25 °C	311:315	arg1	0.86					366:369	0.86	366:369	0.86	366:369	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	20	from	0.86	366:369	arg1	activity					344:351	the water activity	334:351	the water activity from 0.11 to 0.86	334:369	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	20	from	0.86	366:369	arg1	25 °C					311:315	25 °C	311:315	25 °C	311:315	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	20	from	0.86	366:369	arg1	range					325:329	the range	321:329	the range of the water activity from 0.11 to 0.86	321:369	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	6	21	theme	part	986:989	arg1	composition					960:970	chemical composition	951:970	chemical composition of the edible part of the avocado fruit	951:1010	In order to characterize the research material, chemical composition of the edible part of the avocado fruit was determined.					
31972195	0	22	theme	Moisture	0:7	arg1	characteristics					18:32	Moisture sorption characteristics	0:32	Moisture sorption characteristics of food powders containing	0:59	Moisture sorption characteristics of food powders containing freeze dried avocado, maltodextrin and inulin.					
31972195	1	23	theme	salt	293:296	arg1	solutions					298:306	saturated salt solutions	283:306	saturated salt solutions	283:306	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	24	with	isotherms	128:136	arg1	ratio					225:229	different ratio	215:229	different ratio	215:229	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	5	25	theme	sorption	865:872	arg1	data					874:877	the sorption data	861:877	the sorption data of all analyzed blends	861:900	It was found that the Peleg model was the most adequate for representation of the sorption data of all analyzed blends.					
31972195	5	26	theme	Peleg	805:809	arg1	model					811:815	the Peleg model	801:815	the Peleg model	801:815	It was found that the Peleg model was the most adequate for representation of the sorption data of all analyzed blends.					
31972195	5	26	theme	Peleg	805:809	arg1	adequate					830:837	adequate	830:837	adequate	830:837	It was found that the Peleg model was the most adequate for representation of the sorption data of all analyzed blends.					
31972195	1	27	theme	solutions	298:306	arg1	method					273:278	gravimetric static method	254:278	gravimetric static method of saturated salt solutions	254:306	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	7	28	theme	Main	1028:1031	arg1	component					1033:1041	Main component	1028:1041	Main component of the dry matter	1028:1059	Main component of the dry matter was fat.					
31972195	4	29	theme	avocado	693:699	arg1	powder					701:706	the pure avocado powder	684:706	the pure avocado powder	684:706	Sorption isotherm of the pure avocado powder was III type while curves plotted for others powders showed sigmoid shape.					
31972195	6	30	dep	characterize	915:926	arg1	to					912:913	to	912:913	to	912:913	In order to characterize the research material, chemical composition of the edible part of the avocado fruit was determined.					
31972195	1	31	theme	Moisture	108:115	arg1	isotherms					128:136	Moisture adsorption isotherms	108:136	Moisture adsorption isotherms	108:136	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	1	32	theme	different	215:223	arg1	ratio					225:229	different ratio	215:229	different ratio	215:229	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	0	33	theme	powders	42:48	arg1	characteristics					18:32	Moisture sorption characteristics	0:32	Moisture sorption characteristics of food powders containing	0:59	Moisture sorption characteristics of food powders containing freeze dried avocado, maltodextrin and inulin.					
31972195	0	34	dep	freeze	61:66	arg1	dried					68:72	dried	68:72	freeze dried avocado, maltodextrin and inulin	61:105	Moisture sorption characteristics of food powders containing freeze dried avocado, maltodextrin and inulin.					
31972195	6	35	theme	edible	979:984	arg1	part					986:989	the edible part	975:989	the edible part of the avocado fruit	975:1010	In order to characterize the research material, chemical composition of the edible part of the avocado fruit was determined.					
31972195	0	36	theme	food	37:40	arg1	powders					42:48	food powders	37:48	food powders containing	37:59	Moisture sorption characteristics of food powders containing freeze dried avocado, maltodextrin and inulin.					
31972195	1	37	dep	0.86	366:369	arg1	to					363:364	to	363:364	to	363:364	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	3	38	theme	fit	554:556	arg1	goodness					538:545	The goodness	534:545	The goodness of the fit	534:556	The goodness of the fit evaluated on the basis of criteria such as coefficient of the determination and root mean square values.					
31972195	6	39	theme	chemical	951:958	arg1	composition					960:970	chemical composition	951:970	chemical composition of the edible part of the avocado fruit	951:1010	In order to characterize the research material, chemical composition of the edible part of the avocado fruit was determined.					
31972195	2	40	dep	models	420:425	arg1	BET					428:430	BET	428:430	BET	428:430	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	40	dep	models	420:425	arg1	Henderson					523:531	Henderson	523:531	Henderson	523:531	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	40	dep	models	420:425	arg1	Brunauer-Emmett-Teller					433:454	Brunauer-Emmett-Teller	433:454	Brunauer-Emmett-Teller	433:454	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	40	dep	models	420:425	arg1	Lewicki					504:510	Lewicki	504:510	Lewicki	504:510	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	40	dep	models	420:425	arg1	models					420:425	sorption models	411:425	sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson	411:531	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	40	dep	models	420:425	arg1	Oswin					513:517	Oswin	513:517	Oswin	513:517	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	2	40	dep	models	420:425	arg1	GAB					458:460	GAB	458:460	GAB	458:460	The data obtained were evaluated using sorption models: BET (Brunauer-Emmett-Teller), GAB (Guggenheim, Anderson and deBoer), Peleg, Lewicki, Oswin and Henderson.					
31972195	1	41	theme	adsorption	117:126	arg1	isotherms					128:136	Moisture adsorption isotherms	108:136	Moisture adsorption isotherms	108:136	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	7	42	theme	dry	1050:1052	arg1	matter					1054:1059	the dry matter	1046:1059	the dry matter	1046:1059	Main component of the dry matter was fat.					
31972195	3	43	theme	determination	620:632	arg1	values					655:660	root mean square values	638:660	root mean square values	638:660	The goodness of the fit evaluated on the basis of criteria such as coefficient of the determination and root mean square values.					
31972195	3	43	theme	determination	620:632	arg1	coefficient					601:611	coefficient	601:611	coefficient of the determination	601:632	The goodness of the fit evaluated on the basis of criteria such as coefficient of the determination and root mean square values.					
31972195	4	44	theme	others	746:751	arg1	powders					753:759	others powders	746:759	others powders	746:759	Sorption isotherm of the pure avocado powder was III type while curves plotted for others powders showed sigmoid shape.					
31972195	5	45	theme	data	874:877	arg1	representation					843:856	representation	843:856	representation of the sorption data of all analyzed blends	843:900	It was found that the Peleg model was the most adequate for representation of the sorption data of all analyzed blends.					
31972195	1	46	theme	powders	158:164	arg1	isotherms					128:136	Moisture adsorption isotherms	108:136	Moisture adsorption isotherms	108:136	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	3	47	dep	criteria	584:591	arg1	the					571:573	the	571:573	the	571:573	The goodness of the fit evaluated on the basis of criteria such as coefficient of the determination and root mean square values.					
31972195	3	47	dep	criteria	584:591	arg1	basis					575:579	basis	575:579	basis	575:579	The goodness of the fit evaluated on the basis of criteria such as coefficient of the determination and root mean square values.					
31972195	1	48	theme	freeze-dried	145:156	arg1	powders					158:164	the freeze-dried powders	141:164	the freeze-dried powders containing avocado, maltodextrin and inulin	141:208	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
31972195	6	49	theme	research	932:939	arg1	material					941:948	the research material	928:948	the research material	928:948	In order to characterize the research material, chemical composition of the edible part of the avocado fruit was determined.					
31972195	3	50	theme	mean	643:646	arg1	values					655:660	root mean square values	638:660	root mean square values	638:660	The goodness of the fit evaluated on the basis of criteria such as coefficient of the determination and root mean square values.					
31972195	0	51	theme	sorption	9:16	arg1	characteristics					18:32	Moisture sorption characteristics	0:32	Moisture sorption characteristics of food powders containing	0:59	Moisture sorption characteristics of food powders containing freeze dried avocado, maltodextrin and inulin.					
31972195	1	52	theme	gravimetric	254:264	arg1	method					273:278	gravimetric static method	254:278	gravimetric static method of saturated salt solutions	254:306	Moisture adsorption isotherms of the freeze-dried powders containing avocado, maltodextrin and inulin with different ratio, were determined using gravimetric static method of saturated salt solutions at 25 °C and the range of the water activity from 0.11 to 0.86.					
34389393	0	0	with	polysaccharide	30:43	arg1	activity					116:123	potential immunomodulatory activity	89:123	potential immunomodulatory activity	89:123	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.					
34389393	1	1	theme	water	254:258	arg1	extraction					260:269	hot water extraction	250:269	hot water extraction	250:269	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
34389393	5	2	theme	original	976:983	arg1	macrophages					985:995	original macrophages	976:995	original macrophages	976:995	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	0	3	theme	potential	89:97	arg1	activity					116:123	potential immunomodulatory activity	89:123	potential immunomodulatory activity	89:123	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.					
34389393	0	4	from	Characterization	0:15	arg1	Duch					78:81	Duch	78:81	Duch	78:81	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.					
34389393	0	4	from	Characterization	0:15	arg1	pumpkin					50:56	pumpkin	50:56	pumpkin (Cucurbita moschata Duch)	50:82	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.					
34389393	2	5	theme	backbone	623:630	arg1	C-6					632:634	backbone C-6	623:634	backbone C-6	623:634	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	6	dep	infrared	369:376	arg1	analyses					476:483	analyses	476:483	analyses	476:483	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	7	link	linked	561:566	arg1	residues					583:590	α-1,4 linked glucopyranosyl residues	555:590	α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6	555:634	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	1	8	theme	ethanol	272:278	arg1	precipitation					280:292	ethanol precipitation	272:292	ethanol precipitation	272:292	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
34389393	0	9	from	pumpkin	50:56	arg1	Characterization					0:15	Characterization	0:15	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.	0:124	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.					
34389393	0	9	from	pumpkin	50:56	arg1	polysaccharide					30:43	a neutral polysaccharide	20:43	a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity	20:123	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.					
34389393	5	10	from	polarization	960:971	arg1	M2					1036:1037	M2	1036:1037	M2	1036:1037	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	4	11	theme	RAW	834:836	arg1	macrophages					844:854	RAW 264.7 macrophages	834:854	RAW 264.7 macrophages	834:854	In immunostimulation assays, CMDP-1a promoted the proliferation of RAW 264.7 macrophages and significantly enhanced their pinocytic and phagocytic capacity.					
34389393	1	12	theme	molecular	174:182	arg1	9.263 kDa					189:197	molecular mass 9.263 kDa	174:197	molecular mass 9.263 kDa	174:197	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
34389393	1	12	theme	molecular	174:182	arg1	CMDP-1a					165:171	CMDP-1a	165:171	CMDP-1a (molecular mass 9.263 kDa)	165:198	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
34389393	0	13	theme	immunomodulatory	99:114	arg1	activity					116:123	potential immunomodulatory activity	89:123	potential immunomodulatory activity	89:123	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.					
34389393	4	14	theme	phagocytic	903:912	arg1	capacity					914:921	their pinocytic and phagocytic capacity	883:921	their pinocytic and phagocytic capacity	883:921	In immunostimulation assays, CMDP-1a promoted the proliferation of RAW 264.7 macrophages and significantly enhanced their pinocytic and phagocytic capacity.					
34389393	1	15	theme	mass	184:187	arg1	9.263 kDa					189:197	molecular mass 9.263 kDa	174:197	molecular mass 9.263 kDa	174:197	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
34389393	1	15	theme	mass	184:187	arg1	CMDP-1a					165:171	CMDP-1a	165:171	CMDP-1a (molecular mass 9.263 kDa)	165:198	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
34389393	2	16	from	C-6	632:634	arg1	linkage					612:618	α-Glcp residue linkage	597:618	α-Glcp residue linkage at backbone C-6	597:634	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	6	17	theme	potential	1147:1155	arg1	immunomodulator					1157:1171	a potential immunomodulator	1145:1171	a potential immunomodulator for food purposes	1145:1189	These results indicated that CMDP-1a might be a potential immunomodulator for food purposes.					
34389393	6	17	theme	potential	1147:1155	arg1	CMDP-1a					1128:1134	CMDP-1a	1128:1134	CMDP-1a	1128:1134	These results indicated that CMDP-1a might be a potential immunomodulator for food purposes.					
34389393	5	18	from	conversion	1005:1014	arg1	M2					1036:1037	M2	1036:1037	M2	1036:1037	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	3	19	contain	had	725:727	arg1	CMDP-1a					717:723	CMDP-1a	717:723	CMDP-1a	717:723	Atomic force microscopy and scanning electron microscopy analyses revealed that CMDP-1a had a spherical conformation in solution.					
34389393	3	19	contain	had	725:727	arg2	conformation					741:752	a spherical conformation	729:752	a spherical conformation	729:752	Atomic force microscopy and scanning electron microscopy analyses revealed that CMDP-1a had a spherical conformation in solution.					
34389393	5	20	theme	macrophages	1019:1029	arg1	polarization					960:971	the M1 polarization	953:971	the M1 polarization of original macrophages	953:995	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	5	20	theme	macrophages	1019:1029	arg1	conversion					1005:1014	the conversion	1001:1014	the conversion of macrophages from M2 to M1	1001:1043	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	0	21	theme	neutral	22:28	arg1	polysaccharide					30:43	a neutral polysaccharide	20:43	a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity	20:123	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.					
34389393	2	22	theme	magnetic	444:451	arg1	resonance					453:461	two-dimensional nuclear magnetic resonance	420:461	two-dimensional nuclear magnetic resonance spectroscopy	420:474	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	23	theme	resonance	453:461	arg1	methylation					338:348	methylation	338:348	methylation	338:348	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	23	theme	resonance	453:461	arg1	spectroscopy					463:474	two-dimensional nuclear magnetic resonance spectroscopy	420:474	two-dimensional nuclear magnetic resonance spectroscopy	420:474	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	24	theme	glucopyranosyl	568:581	arg1	residues					583:590	α-1,4 linked glucopyranosyl residues	555:590	α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6	555:634	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	25	dep	methylation	338:348	arg1	basis					329:333	basis	329:333	basis	329:333	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	25	dep	methylation	338:348	arg1	the					325:327	the	325:327	the	325:327	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	26	theme	nuclear	436:442	arg1	resonance					453:461	two-dimensional nuclear magnetic resonance	420:461	two-dimensional nuclear magnetic resonance spectroscopy	420:474	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	27	theme	CMDP-1a	503:509	arg1	structure					490:498	the structure	486:498	the structure of CMDP-1a	486:509	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	27	theme	CMDP-1a	503:509	arg1	backbone					534:541	a backbone	532:541	a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6	532:634	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	1	28	theme	column	299:304	arg1	chromatography					306:319	column chromatography	299:319	column chromatography	299:319	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
34389393	2	29	theme	linked	561:566	arg1	residues					583:590	α-1,4 linked glucopyranosyl residues	555:590	α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6	555:634	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	3	30	dep	microscopy	650:659	arg1	analyses					694:701	analyses	694:701	analyses	694:701	Atomic force microscopy and scanning electron microscopy analyses revealed that CMDP-1a had a spherical conformation in solution.					
34389393	5	31	theme	M1/M2	1080:1084	arg1	macrophages					1086:1096	M1/M2 macrophages	1080:1096	M1/M2 macrophages	1080:1096	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	2	32	theme	two-dimensional	420:434	arg1	resonance					453:461	two-dimensional nuclear magnetic resonance	420:461	two-dimensional nuclear magnetic resonance spectroscopy	420:474	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	0	33	theme	polysaccharide	30:43	arg1	Characterization					0:15	Characterization	0:15	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.	0:124	Characterization of a neutral polysaccharide from pumpkin (Cucurbita moschata Duch) with potential immunomodulatory activity.					
34389393	2	34	theme	α-1,4	555:559	arg1	residues					583:590	α-1,4 linked glucopyranosyl residues	555:590	α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6	555:634	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	3	35	theme	scanning	665:672	arg1	microscopy					683:692	scanning electron microscopy	665:692	scanning electron microscopy	665:692	Atomic force microscopy and scanning electron microscopy analyses revealed that CMDP-1a had a spherical conformation in solution.					
34389393	5	36	theme	macrophages	1086:1096	arg1	balance					1069:1075	the balance	1065:1075	the balance of M1/M2 macrophages	1065:1096	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	5	37	theme	M1	957:958	arg1	polarization					960:971	the M1 polarization	953:971	the M1 polarization of original macrophages	953:995	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	3	38	theme	electron	674:681	arg1	microscopy					683:692	scanning electron microscopy	665:692	scanning electron microscopy	665:692	Atomic force microscopy and scanning electron microscopy analyses revealed that CMDP-1a had a spherical conformation in solution.					
34389393	5	39	theme	macrophages	985:995	arg1	polarization					960:971	the M1 polarization	953:971	the M1 polarization of original macrophages	953:995	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	5	39	theme	macrophages	985:995	arg1	conversion					1005:1014	the conversion	1001:1014	the conversion of macrophages from M2 to M1	1001:1043	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	3	40	theme	spherical	731:739	arg1	conformation					741:752	a spherical conformation	729:752	a spherical conformation	729:752	Atomic force microscopy and scanning electron microscopy analyses revealed that CMDP-1a had a spherical conformation in solution.					
34389393	2	41	theme	residue	604:610	arg1	linkage					612:618	α-Glcp residue linkage	597:618	α-Glcp residue linkage at backbone C-6	597:634	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	1	42	attach	isolated	204:211	arg2	polysaccharide					136:149	A neutral polysaccharide	126:149	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa)	126:198	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
34389393	1	42	attach	isolated	204:211	arg1	Duch					237:240	Duch	237:240	Duch	237:240	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
34389393	4	43	theme	pinocytic	889:897	arg1	capacity					914:921	their pinocytic and phagocytic capacity	883:921	their pinocytic and phagocytic capacity	883:921	In immunostimulation assays, CMDP-1a promoted the proliferation of RAW 264.7 macrophages and significantly enhanced their pinocytic and phagocytic capacity.					
34389393	2	44	theme	α-Glcp	597:602	arg1	linkage					612:618	α-Glcp residue linkage	597:618	α-Glcp residue linkage at backbone C-6	597:634	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	4	45	theme	macrophages	844:854	arg1	proliferation					817:829	the proliferation	813:829	the proliferation of RAW 264.7 macrophages	813:854	In immunostimulation assays, CMDP-1a promoted the proliferation of RAW 264.7 macrophages and significantly enhanced their pinocytic and phagocytic capacity.					
34389393	1	46	theme	neutral	128:134	arg1	polysaccharide					136:149	A neutral polysaccharide	126:149	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa)	126:198	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
34389393	5	47	from	M2	1036:1037	arg1	macrophages					1019:1029	macrophages	1019:1029	macrophages from M2	1019:1037	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	5	47	from	M2	1036:1037	arg1	polarization					960:971	the M1 polarization	953:971	the M1 polarization of original macrophages	953:995	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	5	47	from	M2	1036:1037	arg1	conversion					1005:1014	the conversion	1001:1014	the conversion of macrophages from M2 to M1	1001:1043	Furthermore, CMDP-1a induced the M1 polarization of original macrophages and the conversion of macrophages from M2 to M1, thereby modulating the balance of M1/M2 macrophages.					
34389393	2	48	with	residues	583:590	arg1	linkage					612:618	α-Glcp residue linkage	597:618	α-Glcp residue linkage at backbone C-6	597:634	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	4	49	theme	immunostimulation	770:786	arg1	assays					788:793	immunostimulation assays	770:793	immunostimulation assays	770:793	In immunostimulation assays, CMDP-1a promoted the proliferation of RAW 264.7 macrophages and significantly enhanced their pinocytic and phagocytic capacity.					
34389393	3	50	theme	Atomic	637:642	arg1	microscopy					650:659	Atomic force microscopy	637:659	Atomic force microscopy	637:659	Atomic force microscopy and scanning electron microscopy analyses revealed that CMDP-1a had a spherical conformation in solution.					
34389393	2	51	theme	monosaccharide	379:392	arg1	methylation					338:348	methylation	338:348	methylation	338:348	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	2	51	theme	monosaccharide	379:392	arg1	composition					394:404	monosaccharide composition	379:404	monosaccharide composition	379:404	On the basis of methylation, fourier-transform infrared, monosaccharide composition, and one- and two-dimensional nuclear magnetic resonance spectroscopy analyses, the structure of CMDP-1a was determined to be a backbone composed of α-1,4 linked glucopyranosyl residues with α-Glcp residue linkage at backbone C-6.					
34389393	3	52	theme	force	644:648	arg1	microscopy					650:659	Atomic force microscopy	637:659	Atomic force microscopy	637:659	Atomic force microscopy and scanning electron microscopy analyses revealed that CMDP-1a had a spherical conformation in solution.					
34389393	6	53	theme	food	1177:1180	arg1	purposes					1182:1189	food purposes	1177:1189	food purposes	1177:1189	These results indicated that CMDP-1a might be a potential immunomodulator for food purposes.					
34389393	1	54	theme	hot	250:252	arg1	extraction					260:269	hot water extraction	250:269	hot water extraction	250:269	A neutral polysaccharide designated as CMDP-1a (molecular mass 9.263 kDa) was isolated from Cucurbita moschata Duch through hot water extraction, ethanol precipitation, and column chromatography.					
33838804	1	0	theme	antioxidant	271:281	arg1	activities					283:292	antioxidant activities	271:292	antioxidant activities	271:292	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities.					
33838804	1	1	dep	polysaccharides	107:121	arg1	polysaccharides					107:121	Two pectic polysaccharides	96:121	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a)	96:145	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities.					
33838804	1	1	dep	polysaccharides	107:121	arg1	WRSP-A3a					137:144	WRSP-A3a	137:144	WRSP-A3a	137:144	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities.					
33838804	1	1	dep	polysaccharides	107:121	arg1	WRSP-A2b					124:131	WRSP-A2b	124:131	WRSP-A2b	124:131	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities.					
33838804	1	2	theme	activities	283:292	arg1	terms					232:236	terms	232:236	terms of their physical properties and antioxidant activities	232:292	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities.					
33838804	4	3	contain	contains	669:676	arg1	WRSP-A2b					660:667	WRSP-A2b	660:667	WRSP-A2b	660:667	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	4	3	contain	contains	669:676	arg2	domain					701:706	a highly branched RG-I domain	678:706	a highly branched RG-I domain	678:706	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	5	4	theme	scavenging	924:933	arg1	radicals					945:952	scavenging different radicals	924:952	scavenging different radicals	924:952	WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains.					
33838804	4	5	contain	contains	779:786	arg1	WRSP-A3a					770:777	WRSP-A3a	770:777	WRSP-A3a	770:777	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	4	5	contain	contains	779:786	arg2	domain					810:815	a small branched RG-I domain	788:815	a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains	788:870	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	5	6	theme	stronger	891:898	arg1	activity					912:919	stronger antioxidant activity	891:919	stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains	891:1047	WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains.					
33838804	5	7	theme	HG	1038:1039	arg1	domains					1041:1047	HG domains	1038:1047	HG domains	1038:1047	WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains.					
33838804	2	8	theme	2.3:2.9:1	559:567	arg1	ratios					535:540	mass ratios	530:540	mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively	530:581	Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively.					
33838804	5	9	theme	different	935:943	arg1	radicals					945:952	scavenging different radicals	924:952	scavenging different radicals	924:952	WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains.					
33838804	4	10	link	-linked	843:849	arg1	chains					865:870	β-(1→4)-linked galactan side chains	836:870	β-(1→4)-linked galactan side chains	836:870	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	4	11	theme	RG-I	696:699	arg1	domain					701:706	a highly branched RG-I domain	678:706	a highly branched RG-I domain	678:706	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	4	12	theme	-linked	743:749	arg1	arabinans					751:759	α-(1→5)-linked arabinans	736:759	α-(1→5)-linked arabinans	736:759	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	0	13	from	Tonkinensis	43:53	arg1	polysaccharides					7:21	Pectic polysaccharides	0:21	Pectic polysaccharides from Radix Sophorae Tonkinensis	0:53	Pectic polysaccharides from Radix Sophorae Tonkinensis exhibit significant antioxidant effects.					
33838804	2	14	dep	homogalacturonan	431:446	arg1	domains					516:522	domains	516:522	domains	516:522	Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively.					
33838804	6	15	theme	polysaccharide-based	1111:1130	arg1	antioxidants					1132:1143	natural polysaccharide-based antioxidants	1103:1143	natural polysaccharide-based antioxidants	1103:1143	Our results provide useful information for screening natural polysaccharide-based antioxidants from Radix Sophorae Tonkinensis.					
33838804	1	16	theme	pectic	100:105	arg1	polysaccharides					107:121	Two pectic polysaccharides	96:121	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a)	96:145	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities.					
33838804	1	16	theme	pectic	100:105	arg1	WRSP-A3a					137:144	WRSP-A3a	137:144	WRSP-A3a	137:144	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities.					
33838804	1	16	theme	pectic	100:105	arg1	WRSP-A2b					124:131	WRSP-A2b	124:131	WRSP-A2b	124:131	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities.					
33838804	5	17	theme	domains	1041:1047	arg1	content					1009:1015	its higher content	998:1015	its higher content of GalA residues and HG domains	998:1047	WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains.					
33838804	0	18	theme	Pectic	0:5	arg1	polysaccharides					7:21	Pectic polysaccharides	0:21	Pectic polysaccharides from Radix Sophorae Tonkinensis	0:53	Pectic polysaccharides from Radix Sophorae Tonkinensis exhibit significant antioxidant effects.					
33838804	2	19	theme	enzymatic	338:346	arg1	analyses					348:355	enzymatic analyses	338:355	enzymatic analyses	338:355	Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively.					
33838804	2	20	theme	mass	530:533	arg1	ratios					535:540	mass ratios	530:540	mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively	530:581	Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively.					
33838804	4	21	theme	small	790:794	arg1	domain					810:815	a small branched RG-I domain	788:815	a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains	788:870	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	5	22	theme	antioxidant	900:910	arg1	activity					912:919	stronger antioxidant activity	891:919	stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains	891:1047	WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains.					
33838804	4	23	dep	contains	669:676	arg1	whereas					762:768	whereas	762:768	whereas	762:768	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	6	24	theme	natural	1103:1109	arg1	antioxidants					1132:1143	natural polysaccharide-based antioxidants	1103:1143	natural polysaccharide-based antioxidants	1103:1143	Our results provide useful information for screening natural polysaccharide-based antioxidants from Radix Sophorae Tonkinensis.					
33838804	6	25	theme	useful	1070:1075	arg1	information					1077:1087	useful information	1070:1087	useful information for screening natural polysaccharide-based antioxidants from Radix Sophorae Tonkinensis	1070:1175	Our results provide useful information for screening natural polysaccharide-based antioxidants from Radix Sophorae Tonkinensis.					
33838804	5	26	theme	GalA	1020:1023	arg1	residues					1025:1032	GalA residues	1020:1032	GalA residues	1020:1032	WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains.					
33838804	5	27	theme	residues	1025:1032	arg1	content					1009:1015	its higher content	998:1015	its higher content of GalA residues and HG domains	998:1047	WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains.					
33838804	0	28	theme	significant	63:73	arg1	effects					87:93	significant antioxidant effects	63:93	significant antioxidant effects	63:93	Pectic polysaccharides from Radix Sophorae Tonkinensis exhibit significant antioxidant effects.					
33838804	4	29	theme	-linked	843:849	arg1	chains					865:870	β-(1→4)-linked galactan side chains	836:870	β-(1→4)-linked galactan side chains	836:870	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	4	30	theme	branched	796:803	arg1	domain					810:815	a small branched RG-I domain	788:815	a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains	788:870	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	4	31	link	-linked	743:749	arg1	arabinans					751:759	α-(1→5)-linked arabinans	736:759	α-(1→5)-linked arabinans	736:759	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	2	32	theme	0.9:1.8:1	545:553	arg1	ratios					535:540	mass ratios	530:540	mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively	530:581	Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively.					
33838804	4	33	theme	RG-I	805:808	arg1	domain					810:815	a small branched RG-I domain	788:815	a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains	788:870	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	4	34	theme	branched	687:694	arg1	domain					701:706	a highly branched RG-I domain	678:706	a highly branched RG-I domain	678:706	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	5	35	from	activity	912:919	arg1	radicals					945:952	scavenging different radicals	924:952	scavenging different radicals	924:952	WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains.					
33838804	4	36	theme	side	860:863	arg1	chains					865:870	β-(1→4)-linked galactan side chains	836:870	β-(1→4)-linked galactan side chains	836:870	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	2	37	theme	Monosaccharide	295:308	arg1	composition					310:320	Monosaccharide composition	295:320	Monosaccharide composition	295:320	Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively.					
33838804	4	38	theme	galactan	851:858	arg1	chains					865:870	β-(1→4)-linked galactan side chains	836:870	β-(1→4)-linked galactan side chains	836:870	Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains.					
33838804	5	39	theme	higher	1002:1007	arg1	content					1009:1015	its higher content	998:1015	its higher content of GalA residues and HG domains	998:1047	WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains.					
33838804	1	40	theme	physical	247:254	arg1	properties					256:265	their physical properties	241:265	their physical properties	241:265	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities.					
33838804	1	41	theme	properties	256:265	arg1	terms					232:236	terms	232:236	terms of their physical properties and antioxidant activities	232:292	Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities.					
33838804	0	42	theme	antioxidant	75:85	arg1	effects					87:93	significant antioxidant effects	63:93	significant antioxidant effects	63:93	Pectic polysaccharides from Radix Sophorae Tonkinensis exhibit significant antioxidant effects.					
33838804	3	43	theme	RG-I	588:591	arg1	domains					593:599	The RG-I domains	584:599	The RG-I domains	584:599	The RG-I domains were further purified and characterized.					
34361607	0	0	theme	Polysaccharide	72:85	arg1	Compounds					87:95	Polysaccharide Compounds	72:95	Polysaccharide Compounds	72:95	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.					
34361607	1	1	theme	monosaccharides	301:315	arg1	content					274:280	content	274:280	content	274:280	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	1	theme	monosaccharides	301:315	arg1	composition					286:296	composition	286:296	composition	286:296	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	4	2	dep	days	929:932	arg1	968.21					935:940	968.21	935:940	968.21	935:940	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	2	dep	days	929:932	arg1	1029.45					946:952	1029.45	946:952	1029.45	946:952	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	6	3	theme	wine	1413:1416	arg1	polysaccharides					1418:1432	wine polysaccharides	1413:1432	wine polysaccharides	1413:1432	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	1	4	theme	ultrasounds	157:167	arg1	application					131:141	The application	127:141	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes	127:204	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	4	theme	ultrasounds	157:167	arg1	use					214:216	the use	210:216	the use of different pomace contact times	210:250	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	5	5	theme	pectic	1130:1135	arg1	polysaccharides					1137:1151	pectic polysaccharides	1130:1151	pectic polysaccharides	1130:1151	This fact was attributed to a higher extraction in the must during the sonication process and to an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time.					
34361607	1	6	from	composition	286:296	arg1	wines					354:358	wines	354:358	wines	354:358	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	6	from	composition	286:296	arg1	musts					344:348	the musts	340:348	the musts	340:348	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	4	7	theme	medium	661:666	arg1	time					679:682	medium maceration time	661:682	medium maceration time (3 days)	661:691	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	7	theme	medium	661:666	arg1	days					687:690	3 days	685:690	3 days	685:690	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	5	8	theme	polysaccharides	1137:1151	arg1	release					1119:1125	an important release	1106:1125	an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time	1106:1238	This fact was attributed to a higher extraction in the must during the sonication process and to an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time.					
34361607	1	9	theme	different	221:229	arg1	times					246:250	different pomace contact times	221:250	different pomace contact times	221:250	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	10	theme	polysaccharides	321:335	arg1	content					274:280	content	274:280	content	274:280	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	10	theme	polysaccharides	321:335	arg1	composition					286:296	composition	286:296	composition	286:296	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	4	11	dep	that	856:859	arg1	observed					861:868	observed	861:868	observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively)	861:1006	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	1	12	theme	pomace	231:236	arg1	times					246:250	different pomace contact times	221:250	different pomace contact times	221:250	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	0	13	theme	Compounds	87:95	arg1	Extraction					58:67	the Extraction	54:67	the Extraction of Polysaccharide Compounds during Red Wine Vinification	54:124	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.					
34361607	1	14	from	content	274:280	arg1	wines					354:358	wines	354:358	wines	354:358	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	14	from	content	274:280	arg1	musts					344:348	the musts	340:348	the musts	340:348	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	4	15	theme	maceration	668:677	arg1	time					679:682	medium maceration time	661:682	medium maceration time (3 days)	661:691	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	15	theme	maceration	668:677	arg1	days					687:690	3 days	685:690	3 days	685:690	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	1	16	theme	contact	238:244	arg1	times					246:250	different pomace contact times	221:250	different pomace contact times	221:250	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	0	17	theme	Wine	108:111	arg1	Vinification					113:124	Red Wine Vinification	104:124	Red Wine Vinification	104:124	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.					
34361607	3	18	from	content	509:515	arg1	musts					563:567	the musts	559:567	the musts	559:567	The US increased the content of monosaccharides and polysaccharides in the musts by facilitating their extraction from the solid parts during maceration.					
34361607	1	19	from	changes	259:265	arg1	content					274:280	content	274:280	content	274:280	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	19	from	changes	259:265	arg1	composition					286:296	composition	286:296	composition	286:296	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	0	20	theme	Red	104:106	arg1	Vinification					113:124	Red Wine Vinification	104:124	Red Wine Vinification	104:124	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.					
34361607	4	21	theme	rhamnogalacturonan	786:803	arg1	type					805:808	rhamnogalacturonan type II	786:811	rhamnogalacturonan type II (RG-II)	786:819	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	21	theme	rhamnogalacturonan	786:803	arg1	RG-II					814:818	RG-II	814:818	RG-II	814:818	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	21	theme	rhamnogalacturonan	786:803	arg1	polysaccharides					737:751	polysaccharides	737:751	polysaccharides rich in arabinose and galactose	737:783	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	6	22	theme	certain	1395:1401	arg1	levels					1403:1408	certain levels	1395:1408	certain levels of wine polysaccharides	1395:1432	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	2	23	theme	alcoholic	464:472	arg1	fermentation					474:485	the alcoholic fermentation	460:485	the alcoholic fermentation	460:485	These differences were maintained from the moment of pressing (end of maceration) until the end of the alcoholic fermentation.					
34361607	6	24	theme	polysaccharide	1305:1318	arg1	content					1320:1326	the polysaccharide content	1301:1326	the polysaccharide content in the wines	1301:1339	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	1	25	dep	content	274:280	arg1	the					270:272	the	270:272	the	270:272	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	4	26	dep	968.21	935:940	arg1	356.81					975:980	356.81	975:980	356.81	975:980	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	26	dep	968.21	935:940	arg1	1700.50					966:972	1700.50	966:972	1700.50	966:972	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	26	dep	968.21	935:940	arg1	895.04					955:960	895.04	955:960	895.04	955:960	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	26	dep	968.21	935:940	arg1	343.95					986:991	343.95	986:991	343.95	986:991	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	1	27	from	application	131:141	arg1	kHz					180:182	28 kHz	177:182	28 kHz	177:182	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	28	dep	ultrasounds	157:167	arg1	US					170:171	US	170:171	US	170:171	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	2	29	theme	pressing	414:421	arg1	moment					404:409	the moment	400:409	the moment of pressing (end of maceration)	400:441	These differences were maintained from the moment of pressing (end of maceration) until the end of the alcoholic fermentation.					
34361607	1	30	theme	times	246:250	arg1	application					131:141	The application	127:141	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes	127:204	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	30	theme	times	246:250	arg1	use					214:216	the use	210:216	the use of different pomace contact times	210:250	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	2	31	dep	pressing	414:421	arg1	end					424:426	end	424:426	end of maceration	424:440	These differences were maintained from the moment of pressing (end of maceration) until the end of the alcoholic fermentation.					
34361607	0	32	theme	Time	46:49	arg1	Effect					0:5	Effect	0:5	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.	0:125	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.					
34361607	5	33	from	extraction	1046:1055	arg1	the					1060:1062	the	1060:1062	the	1060:1062	This fact was attributed to a higher extraction in the must during the sonication process and to an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time.					
34361607	0	34	theme	Treatment	21:29	arg1	Effect					0:5	Effect	0:5	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.	0:125	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.					
34361607	4	35	theme	control	877:883	arg1	wines					885:889	the control wines	873:889	the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively)	873:1006	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	5	36	theme	pomace	1190:1195	arg1	pressing					1164:1171	the pressing	1160:1171	the pressing of the sonicated pomace, which is reported here for the first time	1160:1238	This fact was attributed to a higher extraction in the must during the sonication process and to an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time.					
34361607	0	37	theme	Sonication	10:19	arg1	Treatment					21:29	Sonication Treatment	10:29	Sonication Treatment	10:29	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.					
34361607	3	38	theme	solid	611:615	arg1	parts					617:621	the solid parts	607:621	the solid parts	607:621	The US increased the content of monosaccharides and polysaccharides in the musts by facilitating their extraction from the solid parts during maceration.					
34361607	4	39	theme	sonicated	696:704	arg1	grapes					706:711	sonicated grapes	696:711	sonicated grapes	696:711	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	5	40	theme	sonicated	1180:1188	arg1	pomace					1190:1195	the sonicated pomace	1176:1195	the sonicated pomace	1176:1195	This fact was attributed to a higher extraction in the must during the sonication process and to an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time.					
34361607	0	41	theme	Maceration	35:44	arg1	Time					46:49	Maceration Time	35:49	Maceration Time	35:49	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.					
34361607	5	42	theme	first	1229:1233	arg1	time					1235:1238	the first time	1225:1238	the first time	1225:1238	This fact was attributed to a higher extraction in the must during the sonication process and to an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time.					
34361607	6	43	dep	useful	1279:1284	arg1	increasing					1290:1299	increasing	1290:1299	increasing the polysaccharide content in the wines	1290:1339	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	6	43	dep	useful	1279:1284	arg1	technology					1259:1268	the US technology	1252:1268	the US technology	1252:1268	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	6	43	dep	useful	1279:1284	arg1	useful					1279:1284	useful	1279:1284	useful	1279:1284	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	6	43	dep	useful	1279:1284	arg1	reducing					1348:1355	reducing	1348:1355	reducing the maceration time needed to achieve certain levels of wine polysaccharides	1348:1432	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	6	44	theme	maceration	1361:1370	arg1	time					1372:1375	the maceration time	1357:1375	the maceration time needed to achieve certain levels of wine polysaccharides	1357:1432	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	2	45	theme	maceration	431:440	arg1	end					424:426	end	424:426	end of maceration	424:440	These differences were maintained from the moment of pressing (end of maceration) until the end of the alcoholic fermentation.					
34361607	5	46	theme	sonication	1080:1089	arg1	process					1091:1097	the sonication process	1076:1097	the sonication process	1076:1097	This fact was attributed to a higher extraction in the must during the sonication process and to an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time.					
34361607	1	47	theme	crushed	191:197	arg1	grapes					199:204	the crushed grapes	187:204	the crushed grapes	187:204	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	4	48	theme	days	929:932	arg1	maceration					913:922	an extended maceration	901:922	an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively)	901:1006	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	3	49	theme	polysaccharides	540:554	arg1	content					509:515	the content	505:515	the content of monosaccharides and polysaccharides in the musts	505:567	The US increased the content of monosaccharides and polysaccharides in the musts by facilitating their extraction from the solid parts during maceration.					
34361607	4	50	from	galactose	775:783	arg1	rich					753:756	rich	753:756	rich	753:756	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	0	51	from	Effect	0:5	arg1	Extraction					58:67	the Extraction	54:67	the Extraction of Polysaccharide Compounds during Red Wine Vinification	54:124	Effect of Sonication Treatment and Maceration Time in the Extraction of Polysaccharide Compounds during Red Wine Vinification.					
34361607	5	52	theme	higher	1039:1044	arg1	extraction					1046:1055	a higher extraction	1037:1055	a higher extraction in the must during the sonication process	1037:1097	This fact was attributed to a higher extraction in the must during the sonication process and to an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time.					
34361607	1	53	from	use	214:216	arg1	kHz					180:182	28 kHz	177:182	28 kHz	177:182	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	54	from	kHz	180:182	arg1	application					131:141	The application	127:141	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes	127:204	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	54	from	kHz	180:182	arg1	ultrasounds					157:167	high-power ultrasounds	146:167	high-power ultrasounds (US) at 28 kHz	146:182	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	1	54	from	kHz	180:182	arg1	use					214:216	the use	210:216	the use of different pomace contact times	210:250	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	4	55	theme	time	679:682	arg1	application					646:656	The application	642:656	The application of medium maceration time (3 days) to sonicated grapes	642:711	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	3	56	from	parts	617:621	arg1	extraction					591:600	their extraction	585:600	their extraction from the solid parts	585:621	The US increased the content of monosaccharides and polysaccharides in the musts by facilitating their extraction from the solid parts during maceration.					
34361607	3	57	theme	monosaccharides	520:534	arg1	content					509:515	the content	505:515	the content of monosaccharides and polysaccharides in the musts	505:567	The US increased the content of monosaccharides and polysaccharides in the musts by facilitating their extraction from the solid parts during maceration.					
34361607	4	58	theme	polysaccharides	737:751	arg1	extraction					723:732	an extraction	720:732	an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP)	720:842	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	59	from	arabinose	761:769	arg1	rich					753:756	rich	753:756	rich	753:756	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	5	60	dep	the	1060:1062	arg1	must					1064:1067	must	1064:1067	must	1064:1067	This fact was attributed to a higher extraction in the must during the sonication process and to an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time.					
34361607	6	61	from	content	1320:1326	arg1	wines					1335:1339	the wines	1331:1339	the wines	1331:1339	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	6	62	theme	US	1256:1257	arg1	increasing					1290:1299	increasing	1290:1299	increasing the polysaccharide content in the wines	1290:1339	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	6	62	theme	US	1256:1257	arg1	technology					1259:1268	the US technology	1252:1268	the US technology	1252:1268	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	6	62	theme	US	1256:1257	arg1	useful					1279:1284	useful	1279:1284	useful	1279:1284	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	6	62	theme	US	1256:1257	arg1	reducing					1348:1355	reducing	1348:1355	reducing the maceration time needed to achieve certain levels of wine polysaccharides	1348:1432	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	4	63	from	rich	753:756	arg1	galactose					775:783	galactose	775:783	galactose	775:783	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	63	from	rich	753:756	arg1	arabinose					761:769	arabinose	761:769	arabinose	761:769	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	5	64	theme	important	1109:1117	arg1	release					1119:1125	an important release	1106:1125	an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time	1106:1238	This fact was attributed to a higher extraction in the must during the sonication process and to an important release of pectic polysaccharides during the pressing of the sonicated pomace, which is reported here for the first time.					
34361607	4	65	theme	extended	904:911	arg1	maceration					913:922	an extended maceration	901:922	an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively)	901:1006	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	66	theme	rich	753:756	arg1	type					805:808	rhamnogalacturonan type II	786:811	rhamnogalacturonan type II (RG-II)	786:819	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	66	theme	rich	753:756	arg1	polysaccharides					737:751	polysaccharides	737:751	polysaccharides rich in arabinose and galactose	737:783	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	4	66	theme	rich	753:756	arg1	mannoproteins					825:837	mannoproteins	825:837	mannoproteins (MP)	825:842	The application of medium maceration time (3 days) to sonicated grapes led to an extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonan type II (RG-II) and mannoproteins (MP), similar to that observed in the control wines made with an extended maceration of 7 days (968.21 vs. 1029.45; 895.04 vs. 1700.50; 356.81 vs. 343.95, respectively).					
34361607	6	67	theme	polysaccharides	1418:1432	arg1	levels					1403:1408	certain levels	1395:1408	certain levels of wine polysaccharides	1395:1432	Therefore, the US technology could be useful for increasing the polysaccharide content in the wines or for reducing the maceration time needed to achieve certain levels of wine polysaccharides.					
34361607	1	68	theme	high-power	146:155	arg1	ultrasounds					157:167	high-power ultrasounds	146:167	high-power ultrasounds (US) at 28 kHz	146:182	The application of high-power ultrasounds (US) at 28 kHz to the crushed grapes and the use of different pomace contact times caused changes in the content and composition of monosaccharides and polysaccharides in the musts and wines.					
34361607	2	69	theme	fermentation	474:485	arg1	end					453:455	the end	449:455	the end of the alcoholic fermentation	449:485	These differences were maintained from the moment of pressing (end of maceration) until the end of the alcoholic fermentation.					
34087304	6	0	theme	tissue	1053:1058	arg1	response					1029:1036	the inflammatory response	1012:1036	the inflammatory response of surrounding tissue	1012:1058	When implanted subcutaneously in rats, the hydrogels containing fucoidan inhibit the inflammatory response of surrounding tissue.					
34087304	3	1	dep	components	635:644	arg1	the					631:633	the	631:633	the	631:633	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	6	2	theme	surrounding	1041:1051	arg1	tissue					1053:1058	surrounding tissue	1041:1058	surrounding tissue	1041:1058	When implanted subcutaneously in rats, the hydrogels containing fucoidan inhibit the inflammatory response of surrounding tissue.					
34087304	4	3	theme	developed	706:714	arg1	hydrogels					716:724	the developed hydrogels	702:724	the developed hydrogels	702:724	Importantly, the developed hydrogels are mechanically robust and the maximum compressive stress is up to 28.37 ± 4.63 kPa.					
34087304	4	3	theme	developed	706:714	arg1	robust					743:748	robust	743:748	robust	743:748	Importantly, the developed hydrogels are mechanically robust and the maximum compressive stress is up to 28.37 ± 4.63 kPa.					
34087304	0	4	theme	biocompatible	120:132	arg1	evaluation					134:143	biocompatible evaluation	120:143	biocompatible evaluation	120:143	Marine polysaccharide-based composite hydrogels containing fucoidan: Preparation, physicochemical characterization, and biocompatible evaluation.					
34087304	7	5	theme	promising	1104:1112	arg1	hydrogels					1090:1098	the designed composite hydrogels	1067:1098	the designed composite hydrogels	1067:1098	Thus, the designed composite hydrogels are promising bio-scaffolds in biomedical applications.					
34087304	7	5	theme	promising	1104:1112	arg1	bio-scaffolds					1114:1126	promising bio-scaffolds	1104:1126	promising bio-scaffolds in biomedical applications	1104:1153	Thus, the designed composite hydrogels are promising bio-scaffolds in biomedical applications.					
34087304	3	6	contain	possess	538:544	arg1	hydrogels					528:536	The prepared hydrogels	515:536	The prepared hydrogels	515:536	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	3	6	contain	possess	538:544	arg2	swelling					580:587	swelling	580:587	swelling	580:587	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	3	6	contain	possess	538:544	arg2	architecture					566:577	tunable microporous architecture	546:577	tunable microporous architecture	546:577	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	3	6	contain	possess	538:544	arg2	properties					608:617	biodegradable properties	594:617	biodegradable properties	594:617	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	0	7	contain	containing	48:57	arg2	fucoidan					59:66	fucoidan	59:66	fucoidan	59:66	Marine polysaccharide-based composite hydrogels containing fucoidan: Preparation, physicochemical characterization, and biocompatible evaluation.					
34087304	0	7	contain	containing	48:57	arg1	hydrogels					38:46	Marine polysaccharide-based composite hydrogels	0:46	Marine polysaccharide-based composite hydrogels containing fucoidan	0:66	Marine polysaccharide-based composite hydrogels containing fucoidan: Preparation, physicochemical characterization, and biocompatible evaluation.					
34087304	5	8	theme	composite	829:837	arg1	hydrogels					839:847	the composite hydrogels	825:847	the composite hydrogels	825:847	Furthermore, the composite hydrogels exhibit excellent cytocompatibility, blood compatibility, and histocompatibility.					
34087304	3	9	theme	tunable	546:552	arg1	architecture					566:577	tunable microporous architecture	546:577	tunable microporous architecture	546:577	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	3	10	theme	prepared	519:526	arg1	hydrogels					528:536	The prepared hydrogels	515:536	The prepared hydrogels	515:536	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	1	11	theme	diversified	214:224	arg1	applications					237:248	diversified biomedical applications	214:248	diversified biomedical applications owing to their excellent (bio)physicochemical properties	214:305	Marine polysaccharide-based hydrogels have drawn much attention for diversified biomedical applications owing to their excellent (bio)physicochemical properties.					
34087304	0	12	theme	Marine	0:5	arg1	hydrogels					38:46	Marine polysaccharide-based composite hydrogels	0:46	Marine polysaccharide-based composite hydrogels containing fucoidan	0:66	Marine polysaccharide-based composite hydrogels containing fucoidan: Preparation, physicochemical characterization, and biocompatible evaluation.					
34087304	1	13	theme	biomedical	226:235	arg1	applications					237:248	diversified biomedical applications	214:248	diversified biomedical applications owing to their excellent (bio)physicochemical properties	214:305	Marine polysaccharide-based hydrogels have drawn much attention for diversified biomedical applications owing to their excellent (bio)physicochemical properties.					
34087304	0	14	theme	polysaccharide-based	7:26	arg1	hydrogels					38:46	Marine polysaccharide-based composite hydrogels	0:46	Marine polysaccharide-based composite hydrogels containing fucoidan	0:66	Marine polysaccharide-based composite hydrogels containing fucoidan: Preparation, physicochemical characterization, and biocompatible evaluation.					
34087304	7	15	theme	designed	1071:1078	arg1	hydrogels					1090:1098	the designed composite hydrogels	1067:1098	the designed composite hydrogels	1067:1098	Thus, the designed composite hydrogels are promising bio-scaffolds in biomedical applications.					
34087304	7	15	theme	designed	1071:1078	arg1	bio-scaffolds					1114:1126	promising bio-scaffolds	1104:1126	promising bio-scaffolds in biomedical applications	1104:1153	Thus, the designed composite hydrogels are promising bio-scaffolds in biomedical applications.					
34087304	2	16	theme	hydrogels	369:377	arg1	series					331:336	a series	329:336	a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan	329:421	In the present work, a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan are prepared via a facile chemical cross-linking approach in an alkali/urea aqueous system.					
34087304	6	17	theme	inflammatory	1016:1027	arg1	response					1029:1036	the inflammatory response	1012:1036	the inflammatory response of surrounding tissue	1012:1058	When implanted subcutaneously in rats, the hydrogels containing fucoidan inhibit the inflammatory response of surrounding tissue.					
34087304	4	18	theme	maximum	758:764	arg1	28.37 ± 4.63 kPa					794:809	28.37 ± 4.63 kPa	794:809	28.37 ± 4.63 kPa	794:809	Importantly, the developed hydrogels are mechanically robust and the maximum compressive stress is up to 28.37 ± 4.63 kPa.					
34087304	4	18	theme	maximum	758:764	arg1	stress					778:783	the maximum compressive stress	754:783	the maximum compressive stress	754:783	Importantly, the developed hydrogels are mechanically robust and the maximum compressive stress is up to 28.37 ± 4.63 kPa.					
34087304	2	19	theme	alkali/urea	487:497	arg1	system					507:512	an alkali/urea aqueous system	484:512	an alkali/urea aqueous system	484:512	In the present work, a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan are prepared via a facile chemical cross-linking approach in an alkali/urea aqueous system.					
34087304	2	20	theme	polysaccharide-based	348:367	arg1	hydrogels					369:377	marine polysaccharide-based hydrogels	341:377	marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan	341:421	In the present work, a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan are prepared via a facile chemical cross-linking approach in an alkali/urea aqueous system.					
34087304	7	21	theme	composite	1080:1088	arg1	hydrogels					1090:1098	the designed composite hydrogels	1067:1098	the designed composite hydrogels	1067:1098	Thus, the designed composite hydrogels are promising bio-scaffolds in biomedical applications.					
34087304	7	21	theme	composite	1080:1088	arg1	bio-scaffolds					1114:1126	promising bio-scaffolds	1104:1126	promising bio-scaffolds in biomedical applications	1104:1153	Thus, the designed composite hydrogels are promising bio-scaffolds in biomedical applications.					
34087304	2	22	theme	marine	341:346	arg1	hydrogels					369:377	marine polysaccharide-based hydrogels	341:377	marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan	341:421	In the present work, a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan are prepared via a facile chemical cross-linking approach in an alkali/urea aqueous system.					
34087304	3	23	theme	marine	665:670	arg1	polysaccharides					672:686	marine polysaccharides	665:686	marine polysaccharides	665:686	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	0	24	theme	composite	28:36	arg1	hydrogels					38:46	Marine polysaccharide-based composite hydrogels	0:46	Marine polysaccharide-based composite hydrogels containing fucoidan	0:66	Marine polysaccharide-based composite hydrogels containing fucoidan: Preparation, physicochemical characterization, and biocompatible evaluation.					
34087304	2	25	theme	cross-linking	458:470	arg1	approach					472:479	a facile chemical cross-linking approach	440:479	a facile chemical cross-linking approach in an alkali/urea aqueous system	440:512	In the present work, a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan are prepared via a facile chemical cross-linking approach in an alkali/urea aqueous system.					
34087304	5	26	theme	blood	886:890	arg1	compatibility					892:904	blood compatibility	886:904	blood compatibility	886:904	Furthermore, the composite hydrogels exhibit excellent cytocompatibility, blood compatibility, and histocompatibility.					
34087304	3	27	theme	microporous	554:564	arg1	architecture					566:577	tunable microporous architecture	546:577	tunable microporous architecture	546:577	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	1	28	theme	excellent	265:273	arg1	properties					296:305	their excellent (bio)physicochemical properties	259:305	their excellent (bio)physicochemical properties	259:305	Marine polysaccharide-based hydrogels have drawn much attention for diversified biomedical applications owing to their excellent (bio)physicochemical properties.					
34087304	0	29	dep	Preparation	69:79	arg1	hydrogels					38:46	Marine polysaccharide-based composite hydrogels	0:46	Marine polysaccharide-based composite hydrogels containing fucoidan	0:66	Marine polysaccharide-based composite hydrogels containing fucoidan: Preparation, physicochemical characterization, and biocompatible evaluation.					
34087304	2	30	theme	chemical	449:456	arg1	approach					472:479	a facile chemical cross-linking approach	440:479	a facile chemical cross-linking approach in an alkali/urea aqueous system	440:512	In the present work, a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan are prepared via a facile chemical cross-linking approach in an alkali/urea aqueous system.					
34087304	7	31	from	bio-scaffolds	1114:1126	arg1	applications					1142:1153	biomedical applications	1131:1153	biomedical applications	1131:1153	Thus, the designed composite hydrogels are promising bio-scaffolds in biomedical applications.					
34087304	3	32	theme	polysaccharides	672:686	arg1	proportions					650:660	proportions	650:660	proportions	650:660	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	3	32	theme	polysaccharides	672:686	arg1	components					635:644	components	635:644	components	635:644	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	2	33	theme	facile	442:447	arg1	approach					472:479	a facile chemical cross-linking approach	440:479	a facile chemical cross-linking approach in an alkali/urea aqueous system	440:512	In the present work, a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan are prepared via a facile chemical cross-linking approach in an alkali/urea aqueous system.					
34087304	5	34	theme	excellent	857:865	arg1	cytocompatibility					867:883	excellent cytocompatibility	857:883	excellent cytocompatibility	857:883	Furthermore, the composite hydrogels exhibit excellent cytocompatibility, blood compatibility, and histocompatibility.					
34087304	1	35	theme	Marine	146:151	arg1	hydrogels					174:182	Marine polysaccharide-based hydrogels	146:182	Marine polysaccharide-based hydrogels	146:182	Marine polysaccharide-based hydrogels have drawn much attention for diversified biomedical applications owing to their excellent (bio)physicochemical properties.					
34087304	1	36	theme	polysaccharide-based	153:172	arg1	hydrogels					174:182	Marine polysaccharide-based hydrogels	146:182	Marine polysaccharide-based hydrogels	146:182	Marine polysaccharide-based hydrogels have drawn much attention for diversified biomedical applications owing to their excellent (bio)physicochemical properties.					
34087304	2	37	theme	present	315:321	arg1	work					323:326	the present work	311:326	the present work	311:326	In the present work, a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan are prepared via a facile chemical cross-linking approach in an alkali/urea aqueous system.					
34087304	6	38	contain	containing	984:993	arg2	fucoidan					995:1002	fucoidan	995:1002	fucoidan	995:1002	When implanted subcutaneously in rats, the hydrogels containing fucoidan inhibit the inflammatory response of surrounding tissue.					
34087304	6	38	contain	containing	984:993	arg1	hydrogels					974:982	the hydrogels	970:982	the hydrogels containing fucoidan	970:1002	When implanted subcutaneously in rats, the hydrogels containing fucoidan inhibit the inflammatory response of surrounding tissue.					
34087304	4	39	dep	28.37 ± 4.63 kPa	794:809	arg1	up					788:789	up	788:789	up	788:789	Importantly, the developed hydrogels are mechanically robust and the maximum compressive stress is up to 28.37 ± 4.63 kPa.					
34087304	1	40	theme	bio	276:278	arg1	properties					296:305	their excellent (bio)physicochemical properties	259:305	their excellent (bio)physicochemical properties	259:305	Marine polysaccharide-based hydrogels have drawn much attention for diversified biomedical applications owing to their excellent (bio)physicochemical properties.					
34087304	3	41	theme	biodegradable	594:606	arg1	properties					608:617	biodegradable properties	594:617	biodegradable properties	594:617	The prepared hydrogels possess tunable microporous architecture, swelling, and biodegradable properties by changing the components and proportions of marine polysaccharides.					
34087304	7	42	theme	biomedical	1131:1140	arg1	applications					1142:1153	biomedical applications	1131:1153	biomedical applications	1131:1153	Thus, the designed composite hydrogels are promising bio-scaffolds in biomedical applications.					
34087304	4	43	theme	compressive	766:776	arg1	28.37 ± 4.63 kPa					794:809	28.37 ± 4.63 kPa	794:809	28.37 ± 4.63 kPa	794:809	Importantly, the developed hydrogels are mechanically robust and the maximum compressive stress is up to 28.37 ± 4.63 kPa.					
34087304	4	43	theme	compressive	766:776	arg1	stress					778:783	the maximum compressive stress	754:783	the maximum compressive stress	754:783	Importantly, the developed hydrogels are mechanically robust and the maximum compressive stress is up to 28.37 ± 4.63 kPa.					
34087304	0	44	theme	physicochemical	82:96	arg1	characterization					98:113	physicochemical characterization	82:113	physicochemical characterization	82:113	Marine polysaccharide-based composite hydrogels containing fucoidan: Preparation, physicochemical characterization, and biocompatible evaluation.					
34087304	1	45	theme	physicochemical	280:294	arg1	properties					296:305	their excellent (bio)physicochemical properties	259:305	their excellent (bio)physicochemical properties	259:305	Marine polysaccharide-based hydrogels have drawn much attention for diversified biomedical applications owing to their excellent (bio)physicochemical properties.					
34087304	2	46	theme	aqueous	499:505	arg1	system					507:512	an alkali/urea aqueous system	484:512	an alkali/urea aqueous system	484:512	In the present work, a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan are prepared via a facile chemical cross-linking approach in an alkali/urea aqueous system.					
34087304	2	47	from	approach	472:479	arg1	system					507:512	an alkali/urea aqueous system	484:512	an alkali/urea aqueous system	484:512	In the present work, a series of marine polysaccharide-based hydrogels composed of chitosan, alginate, or fucoidan are prepared via a facile chemical cross-linking approach in an alkali/urea aqueous system.					
34087304	1	48	theme	much	195:198	arg1	attention					200:208	much attention	195:208	much attention	195:208	Marine polysaccharide-based hydrogels have drawn much attention for diversified biomedical applications owing to their excellent (bio)physicochemical properties.					
32643784	0	0	theme	wheat	106:110	arg1	bran					112:115	wheat bran	106:115	wheat bran	106:115	Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.					
32643784	5	1	theme	P38	740:742	arg1	phosphorylation					721:735	the phosphorylation	717:735	the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells	717:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	6	2	with	residues	1050:1057	arg1	points					1074:1079	branching points	1064:1079	branching points at C-6	1064:1086	Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6.					
32643784	4	3	theme	various	663:669	arg1	cytokines					671:679	various cytokines	663:679	various cytokines	663:679	The WBP-F stimulates the RAW264.7 cells through the production of nitric oxide and various cytokines.					
32643784	10	4	theme	wheat	1481:1485	arg1	bran					1487:1490	wheat bran	1481:1490	wheat bran	1481:1490	The results found in this study revealed that the purified polysaccharide from wheat bran potentially enhanced the RAW264.7 cells activation.					
32643784	5	5	from	JNK	745:747	arg1	cells					777:781	RAW264.7 cells	768:781	RAW264.7 cells	768:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	8	6	contain	containing	1233:1242	arg1	bran					1228:1231	wheat bran	1222:1231	wheat bran containing numerous beneficial activities	1222:1273	It has been reported that polysaccharides of wheat bran containing numerous beneficial activities.					
32643784	8	6	contain	containing	1233:1242	arg2	activities					1264:1273	numerous beneficial activities	1244:1273	numerous beneficial activities	1244:1273	It has been reported that polysaccharides of wheat bran containing numerous beneficial activities.					
32643784	6	7	theme	4-α-D-linked	1022:1033	arg1	residues					1050:1057	4-α-D-linked glucopyranosyl residues	1022:1057	4-α-D-linked glucopyranosyl residues with branching points at C-6	1022:1086	Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6.					
32643784	6	8	theme	glucopyranosyl	1035:1048	arg1	residues					1050:1057	4-α-D-linked glucopyranosyl residues	1022:1057	4-α-D-linked glucopyranosyl residues with branching points at C-6	1022:1086	Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6.					
32643784	10	9	from	bran	1487:1490	arg1	polysaccharide					1461:1474	the purified polysaccharide	1448:1474	the purified polysaccharide from wheat bran	1448:1490	The results found in this study revealed that the purified polysaccharide from wheat bran potentially enhanced the RAW264.7 cells activation.					
32643784	7	10	theme	compounds	1166:1174	arg1	bran					1119:1122	Wheat bran	1113:1122	Wheat bran	1113:1122	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.					
32643784	7	10	theme	compounds	1166:1174	arg1	source					1139:1144	a potential source	1127:1144	a potential source of health-promoting compounds	1127:1174	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.					
32643784	11	11	theme	immunity-enhancing	1603:1620	arg1	polysaccharides					1557:1571	these polysaccharides	1551:1571	these polysaccharides	1551:1571	Hence, these polysaccharides could be utilized as a potent immunity-enhancing agent in food and pharmaceutical industries.					
32643784	11	11	theme	immunity-enhancing	1603:1620	arg1	agent					1622:1626	a potent immunity-enhancing agent	1594:1626	a potent immunity-enhancing agent in food and pharmaceutical industries	1594:1664	Hence, these polysaccharides could be utilized as a potent immunity-enhancing agent in food and pharmaceutical industries.					
32643784	5	12	theme	WBP-F	699:703	arg1	treatment					686:694	The treatment	682:694	The treatment of WBP-F	682:703	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	3	13	theme	WBP-F	513:517	arg1	g/mol					559:563	911.7 and 510.2 × 103 g/mol	537:563	911.7 and 510.2 × 103 g/mol	537:563	The molecular weight (Mw ) of WBP and WBP-F was calculated as 911.7 and 510.2 × 103 g/mol, respectively.					
32643784	3	13	theme	WBP-F	513:517	arg1	Mw					497:498	Mw	497:498	Mw	497:498	The molecular weight (Mw ) of WBP and WBP-F was calculated as 911.7 and 510.2 × 103 g/mol, respectively.					
32643784	3	13	theme	WBP-F	513:517	arg1	weight					489:494	The molecular weight	475:494	The molecular weight (Mw ) of WBP and WBP-F	475:517	The molecular weight (Mw ) of WBP and WBP-F was calculated as 911.7 and 510.2 × 103 g/mol, respectively.					
32643784	6	14	theme	branching	1064:1072	arg1	points					1074:1079	branching points	1064:1079	branching points at C-6	1064:1086	Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6.					
32643784	7	15	theme	potential	1129:1137	arg1	bran					1119:1122	Wheat bran	1113:1122	Wheat bran	1113:1122	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.					
32643784	7	15	theme	potential	1129:1137	arg1	source					1139:1144	a potential source	1127:1144	a potential source of health-promoting compounds	1127:1174	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.					
32643784	8	16	theme	beneficial	1253:1262	arg1	activities					1264:1273	numerous beneficial activities	1244:1273	numerous beneficial activities	1244:1273	It has been reported that polysaccharides of wheat bran containing numerous beneficial activities.					
32643784	10	17	theme	purified	1452:1459	arg1	polysaccharide					1461:1474	the purified polysaccharide	1448:1474	the purified polysaccharide from wheat bran	1448:1490	The results found in this study revealed that the purified polysaccharide from wheat bran potentially enhanced the RAW264.7 cells activation.					
32643784	8	18	theme	numerous	1244:1251	arg1	activities					1264:1273	numerous beneficial activities	1244:1273	numerous beneficial activities	1244:1273	It has been reported that polysaccharides of wheat bran containing numerous beneficial activities.					
32643784	2	19	theme	fraction	313:320	arg1	composition					281:291	The chemical composition	268:291	The chemical composition of WBP and purified fraction (WBP-F)	268:328	The chemical composition of WBP and purified fraction (WBP-F) mainly consists of neutral sugars (91.2 ± 1.2 and 98.7 ± 1.2%), proteins (8.6 ± 0.3 and 0.2 ± 0.1%) and uronic acids (0.7 ± 0.1 and 0.6 ± 0.1%).					
32643784	11	20	from	agent	1622:1626	arg1	industries					1655:1664	food and pharmaceutical industries	1631:1664	food and pharmaceutical industries	1631:1664	Hence, these polysaccharides could be utilized as a potent immunity-enhancing agent in food and pharmaceutical industries.					
32643784	11	21	used	utilized	1582:1589	arg2	agent					1622:1626	a potent immunity-enhancing agent	1594:1626	a potent immunity-enhancing agent in food and pharmaceutical industries	1594:1664	Hence, these polysaccharides could be utilized as a potent immunity-enhancing agent in food and pharmaceutical industries.					
32643784	11	21	used	utilized	1582:1589	arg2	polysaccharides					1557:1571	these polysaccharides	1551:1571	these polysaccharides	1551:1571	Hence, these polysaccharides could be utilized as a potent immunity-enhancing agent in food and pharmaceutical industries.					
32643784	5	22	theme	JNK	745:747	arg1	phosphorylation					721:735	the phosphorylation	717:735	the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells	717:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	2	23	theme	purified	304:311	arg1	WBP-F					323:327	WBP-F	323:327	WBP-F	323:327	The chemical composition of WBP and purified fraction (WBP-F) mainly consists of neutral sugars (91.2 ± 1.2 and 98.7 ± 1.2%), proteins (8.6 ± 0.3 and 0.2 ± 0.1%) and uronic acids (0.7 ± 0.1 and 0.6 ± 0.1%).					
32643784	2	23	theme	purified	304:311	arg1	fraction					313:320	purified fraction	304:320	purified fraction (WBP-F)	304:328	The chemical composition of WBP and purified fraction (WBP-F) mainly consists of neutral sugars (91.2 ± 1.2 and 98.7 ± 1.2%), proteins (8.6 ± 0.3 and 0.2 ± 0.1%) and uronic acids (0.7 ± 0.1 and 0.6 ± 0.1%).					
32643784	5	24	from	NF-ƘB	759:763	arg1	cells					777:781	RAW264.7 cells	768:781	RAW264.7 cells	768:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	7	25	dep	APPLICATIONS	1099:1110	arg1	bran					1119:1122	Wheat bran	1113:1122	Wheat bran	1113:1122	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.					
32643784	7	25	dep	APPLICATIONS	1099:1110	arg1	source					1139:1144	a potential source	1127:1144	a potential source of health-promoting compounds	1127:1174	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.					
32643784	5	26	theme	RAW264.7	820:827	arg1	cells					829:833	RAW264.7 cells	820:833	RAW264.7 cells	820:833	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	5	27	theme	NF-ƘB	759:763	arg1	phosphorylation					721:735	the phosphorylation	717:735	the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells	717:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	6	28	theme	WBP-F	926:930	arg1	details					915:921	the structural details	900:921	the structural details of WBP-F	900:930	Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6.					
32643784	0	29	from	bran	112:115	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.					
32643784	0	29	from	bran	112:115	arg1	elucidation					23:33	structural elucidation	12:33	structural elucidation	12:33	Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.					
32643784	0	29	from	bran	112:115	arg1	properties					57:66	immunostimulating properties	39:66	immunostimulating properties	39:66	Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.					
32643784	6	30	theme	structural	904:913	arg1	details					915:921	the structural details	900:921	the structural details of WBP-F	900:930	Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6.					
32643784	11	31	theme	food	1631:1634	arg1	industries					1655:1664	food and pharmaceutical industries	1631:1664	food and pharmaceutical industries	1631:1664	Hence, these polysaccharides could be utilized as a potent immunity-enhancing agent in food and pharmaceutical industries.					
32643784	6	32	theme	main	996:999	arg1	backbone					1001:1008	the main backbone	992:1008	the main backbone	992:1008	Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6.					
32643784	2	33	theme	uronic	434:439	arg1	acids					441:445	uronic acids	434:445	uronic acids (0.7 ± 0.1 and 0.6 ± 0.1%)	434:472	The chemical composition of WBP and purified fraction (WBP-F) mainly consists of neutral sugars (91.2 ± 1.2 and 98.7 ± 1.2%), proteins (8.6 ± 0.3 and 0.2 ± 0.1%) and uronic acids (0.7 ± 0.1 and 0.6 ± 0.1%).					
32643784	2	33	theme	uronic	434:439	arg1	%					471:471	0.7 ± 0.1 and 0.6 ± 0.1%	448:471	0.7 ± 0.1 and 0.6 ± 0.1%	448:471	The chemical composition of WBP and purified fraction (WBP-F) mainly consists of neutral sugars (91.2 ± 1.2 and 98.7 ± 1.2%), proteins (8.6 ± 0.3 and 0.2 ± 0.1%) and uronic acids (0.7 ± 0.1 and 0.6 ± 0.1%).					
32643784	9	34	theme	wheat	1295:1299	arg1	bran					1301:1304	the wheat bran	1291:1304	the wheat bran polysaccharide	1291:1319	In this study, the wheat bran polysaccharide was extracted, fractionated and investigated their immunostimulatory activities.					
32643784	1	35	theme	structural	207:216	arg1	characteristics					218:232	their structural characteristics	201:232	their structural characteristics	201:232	A water-soluble polysaccharide was extracted from wheat bran (WBP) and investigate their structural characteristics and immunostimulatory activities.					
32643784	0	36	theme	structural	12:21	arg1	elucidation					23:33	structural elucidation	12:33	structural elucidation	12:33	Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.					
32643784	9	37	theme	bran	1301:1304	arg1	polysaccharide					1306:1319	the wheat bran polysaccharide	1291:1319	the wheat bran polysaccharide	1291:1319	In this study, the wheat bran polysaccharide was extracted, fractionated and investigated their immunostimulatory activities.					
32643784	5	38	dep	NF-ƙB	861:865	arg1	pathways					877:884	pathways	877:884	pathways	877:884	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	6	39	dep	GC-MS	948:952	arg1	spectrum					962:969	spectrum	962:969	spectrum	962:969	Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6.					
32643784	1	40	theme	water-soluble	120:132	arg1	polysaccharide					134:147	A water-soluble polysaccharide	118:147	A water-soluble polysaccharide	118:147	A water-soluble polysaccharide was extracted from wheat bran (WBP) and investigate their structural characteristics and immunostimulatory activities.					
32643784	1	41	theme	wheat	168:172	arg1	bran					174:177	wheat bran	168:177	wheat bran (WBP)	168:183	A water-soluble polysaccharide was extracted from wheat bran (WBP) and investigate their structural characteristics and immunostimulatory activities.					
32643784	1	41	theme	wheat	168:172	arg1	WBP					180:182	WBP	180:182	WBP	180:182	A water-soluble polysaccharide was extracted from wheat bran (WBP) and investigate their structural characteristics and immunostimulatory activities.					
32643784	0	42	theme	immunostimulating	39:55	arg1	properties					57:66	immunostimulating properties	39:66	immunostimulating properties	39:66	Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.					
32643784	3	43	theme	molecular	479:487	arg1	g/mol					559:563	911.7 and 510.2 × 103 g/mol	537:563	911.7 and 510.2 × 103 g/mol	537:563	The molecular weight (Mw ) of WBP and WBP-F was calculated as 911.7 and 510.2 × 103 g/mol, respectively.					
32643784	3	43	theme	molecular	479:487	arg1	Mw					497:498	Mw	497:498	Mw	497:498	The molecular weight (Mw ) of WBP and WBP-F was calculated as 911.7 and 510.2 × 103 g/mol, respectively.					
32643784	3	43	theme	molecular	479:487	arg1	weight					489:494	The molecular weight	475:494	The molecular weight (Mw ) of WBP and WBP-F	475:517	The molecular weight (Mw ) of WBP and WBP-F was calculated as 911.7 and 510.2 × 103 g/mol, respectively.					
32643784	2	44	theme	chemical	272:279	arg1	composition					281:291	The chemical composition	268:291	The chemical composition of WBP and purified fraction (WBP-F)	268:328	The chemical composition of WBP and purified fraction (WBP-F) mainly consists of neutral sugars (91.2 ± 1.2 and 98.7 ± 1.2%), proteins (8.6 ± 0.3 and 0.2 ± 0.1%) and uronic acids (0.7 ± 0.1 and 0.6 ± 0.1%).					
32643784	10	45	located	found	1414:1418	arg2	results					1406:1412	The results	1402:1412	The results found in this study	1402:1432	The results found in this study revealed that the purified polysaccharide from wheat bran potentially enhanced the RAW264.7 cells activation.					
32643784	10	45	located	found	1414:1418	arg1	study					1428:1432	this study	1423:1432	this study	1423:1432	The results found in this study revealed that the purified polysaccharide from wheat bran potentially enhanced the RAW264.7 cells activation.					
32643784	7	46	theme	Wheat	1113:1117	arg1	bran					1119:1122	Wheat bran	1113:1122	Wheat bran	1113:1122	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.					
32643784	7	46	theme	Wheat	1113:1117	arg1	source					1139:1144	a potential source	1127:1144	a potential source of health-promoting compounds	1127:1174	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.					
32643784	1	47	theme	immunostimulatory	238:254	arg1	activities					256:265	immunostimulatory activities	238:265	immunostimulatory activities	238:265	A water-soluble polysaccharide was extracted from wheat bran (WBP) and investigate their structural characteristics and immunostimulatory activities.					
32643784	5	48	theme	ERK	750:752	arg1	phosphorylation					721:735	the phosphorylation	717:735	the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells	717:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	10	49	theme	RAW264.7	1517:1524	arg1	activation					1532:1541	the RAW264.7 cells activation	1513:1541	the RAW264.7 cells activation	1513:1541	The results found in this study revealed that the purified polysaccharide from wheat bran potentially enhanced the RAW264.7 cells activation.					
32643784	7	50	theme	health-promoting	1149:1164	arg1	compounds					1166:1174	health-promoting compounds	1149:1174	health-promoting compounds	1149:1174	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.					
32643784	5	51	theme	NF-ƙB	861:865	arg1	activation					847:856	the activation	843:856	the activation of NF-ƙB and MAPKs pathways	843:884	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	7	52	theme	PRACTICAL	1089:1097	arg1	APPLICATIONS					1099:1110	PRACTICAL APPLICATIONS	1089:1110	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.	1089:1175	PRACTICAL APPLICATIONS: Wheat bran is a potential source of health-promoting compounds.					
32643784	11	53	theme	pharmaceutical	1640:1653	arg1	industries					1655:1664	food and pharmaceutical industries	1631:1664	food and pharmaceutical industries	1631:1664	Hence, these polysaccharides could be utilized as a potent immunity-enhancing agent in food and pharmaceutical industries.					
32643784	5	54	from	P38	740:742	arg1	cells					777:781	RAW264.7 cells	768:781	RAW264.7 cells	768:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	5	55	from	cells	777:781	arg1	phosphorylation					721:735	the phosphorylation	717:735	the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells	717:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	4	56	theme	cytokines	671:679	arg1	production					632:641	the production	628:641	the production of nitric oxide and various cytokines	628:679	The WBP-F stimulates the RAW264.7 cells through the production of nitric oxide and various cytokines.					
32643784	6	57	link	4-α-D-linked	1022:1033	arg1	residues					1050:1057	4-α-D-linked glucopyranosyl residues	1022:1057	4-α-D-linked glucopyranosyl residues with branching points at C-6	1022:1086	Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6.					
32643784	5	58	theme	MAPKs	871:875	arg1	activation					847:856	the activation	843:856	the activation of NF-ƙB and MAPKs pathways	843:884	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	8	59	theme	bran	1228:1231	arg1	polysaccharides					1203:1217	polysaccharides	1203:1217	polysaccharides of wheat bran containing numerous beneficial activities	1203:1273	It has been reported that polysaccharides of wheat bran containing numerous beneficial activities.					
32643784	3	60	theme	WBP	505:507	arg1	g/mol					559:563	911.7 and 510.2 × 103 g/mol	537:563	911.7 and 510.2 × 103 g/mol	537:563	The molecular weight (Mw ) of WBP and WBP-F was calculated as 911.7 and 510.2 × 103 g/mol, respectively.					
32643784	3	60	theme	WBP	505:507	arg1	Mw					497:498	Mw	497:498	Mw	497:498	The molecular weight (Mw ) of WBP and WBP-F was calculated as 911.7 and 510.2 × 103 g/mol, respectively.					
32643784	3	60	theme	WBP	505:507	arg1	weight					489:494	The molecular weight	475:494	The molecular weight (Mw ) of WBP and WBP-F	475:517	The molecular weight (Mw ) of WBP and WBP-F was calculated as 911.7 and 510.2 × 103 g/mol, respectively.					
32643784	5	61	from	phosphorylation	721:735	arg1	cells					777:781	RAW264.7 cells	768:781	RAW264.7 cells	768:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	2	62	theme	WBP	296:298	arg1	composition					281:291	The chemical composition	268:291	The chemical composition of WBP and purified fraction (WBP-F)	268:328	The chemical composition of WBP and purified fraction (WBP-F) mainly consists of neutral sugars (91.2 ± 1.2 and 98.7 ± 1.2%), proteins (8.6 ± 0.3 and 0.2 ± 0.1%) and uronic acids (0.7 ± 0.1 and 0.6 ± 0.1%).					
32643784	2	63	theme	neutral	349:355	arg1	%					390:390	91.2 ± 1.2 and 98.7 ± 1.2%	365:390	91.2 ± 1.2 and 98.7 ± 1.2%	365:390	The chemical composition of WBP and purified fraction (WBP-F) mainly consists of neutral sugars (91.2 ± 1.2 and 98.7 ± 1.2%), proteins (8.6 ± 0.3 and 0.2 ± 0.1%) and uronic acids (0.7 ± 0.1 and 0.6 ± 0.1%).					
32643784	2	63	theme	neutral	349:355	arg1	sugars					357:362	neutral sugars	349:362	neutral sugars (91.2 ± 1.2 and 98.7 ± 1.2%)	349:391	The chemical composition of WBP and purified fraction (WBP-F) mainly consists of neutral sugars (91.2 ± 1.2 and 98.7 ± 1.2%), proteins (8.6 ± 0.3 and 0.2 ± 0.1%) and uronic acids (0.7 ± 0.1 and 0.6 ± 0.1%).					
32643784	0	64	theme	polysaccharides	85:99	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.					
32643784	0	64	theme	polysaccharides	85:99	arg1	elucidation					23:33	structural elucidation	12:33	structural elucidation	12:33	Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.					
32643784	0	64	theme	polysaccharides	85:99	arg1	properties					57:66	immunostimulating properties	39:66	immunostimulating properties	39:66	Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.					
32643784	4	65	theme	RAW264.7	605:612	arg1	cells					614:618	the RAW264.7 cells	601:618	the RAW264.7 cells	601:618	The WBP-F stimulates the RAW264.7 cells through the production of nitric oxide and various cytokines.					
32643784	8	66	theme	wheat	1222:1226	arg1	bran					1228:1231	wheat bran	1222:1231	wheat bran containing numerous beneficial activities	1222:1273	It has been reported that polysaccharides of wheat bran containing numerous beneficial activities.					
32643784	4	67	theme	oxide	653:657	arg1	production					632:641	the production	628:641	the production of nitric oxide and various cytokines	628:679	The WBP-F stimulates the RAW264.7 cells through the production of nitric oxide and various cytokines.					
32643784	10	68	theme	cells	1526:1530	arg1	activation					1532:1541	the RAW264.7 cells activation	1513:1541	the RAW264.7 cells activation	1513:1541	The results found in this study revealed that the purified polysaccharide from wheat bran potentially enhanced the RAW264.7 cells activation.					
32643784	0	69	theme	water-soluble	71:83	arg1	polysaccharides					85:99	water-soluble polysaccharides	71:99	water-soluble polysaccharides	71:99	Extraction, structural elucidation and immunostimulating properties of water-soluble polysaccharides from wheat bran.					
32643784	5	70	from	ERK	750:752	arg1	cells					777:781	RAW264.7 cells	768:781	RAW264.7 cells	768:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	5	71	theme	RAW264.7	768:775	arg1	cells					777:781	RAW264.7 cells	768:781	RAW264.7 cells	768:781	The treatment of WBP-F facilitated the phosphorylation of P38, JNK, ERK, and NF-ƘB in RAW264.7 cells suggesting that they might stimulate RAW264.7 cells through the activation of NF-ƙB and MAPKs pathways.					
32643784	11	72	theme	potent	1596:1601	arg1	polysaccharides					1557:1571	these polysaccharides	1551:1571	these polysaccharides	1551:1571	Hence, these polysaccharides could be utilized as a potent immunity-enhancing agent in food and pharmaceutical industries.					
32643784	11	72	theme	potent	1596:1601	arg1	agent					1622:1626	a potent immunity-enhancing agent	1594:1626	a potent immunity-enhancing agent in food and pharmaceutical industries	1594:1664	Hence, these polysaccharides could be utilized as a potent immunity-enhancing agent in food and pharmaceutical industries.					
32643784	4	73	theme	nitric	646:651	arg1	oxide					653:657	nitric oxide	646:657	nitric oxide	646:657	The WBP-F stimulates the RAW264.7 cells through the production of nitric oxide and various cytokines.					
32643784	9	74	theme	immunostimulatory	1372:1388	arg1	activities					1390:1399	their immunostimulatory activities	1366:1399	their immunostimulatory activities	1366:1399	In this study, the wheat bran polysaccharide was extracted, fractionated and investigated their immunostimulatory activities.					
32643784	6	75	from	C-6	1084:1086	arg1	points					1074:1079	branching points	1064:1079	branching points at C-6	1064:1086	Furthermore, the structural details of WBP-F were studied by GC-MS and NMR spectrum, which confirms that the main backbone consists of 4-α-D-linked glucopyranosyl residues with branching points at C-6.					
33636265	1	0	theme	structural	341:350	arg1	barrier					365:371	physical and chemical, structural, mechanical, barrier, and morphological properties	318:401	barrier	365:371	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	1	1	theme	gum	170:172	arg1	KO/SA					201:205	KO/SA	201:205	KO/SA	201:205	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	1	1	theme	gum	170:172	arg1	alginate					191:198	gum kondagogu/sodium alginate	170:198	gum kondagogu/sodium alginate (KO/SA)	170:206	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	1	2	theme	kondagogu/sodium	174:189	arg1	KO/SA					201:205	KO/SA	201:205	KO/SA	201:205	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	1	2	theme	kondagogu/sodium	174:189	arg1	alginate					191:198	gum kondagogu/sodium alginate	170:198	gum kondagogu/sodium alginate (KO/SA)	170:206	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	1	3	theme	optimum	265:271	arg1	ratio					282:286	their optimum blending ratio	259:286	their optimum blending ratio	259:286	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	0	4	theme	packaging	109:117	arg1	films					119:123	flexible packaging films	100:123	flexible packaging films	100:123	Biomacromolecule assembly based on gum kondagogu-sodium alginate composites and their expediency in flexible packaging films.					
33636265	7	5	theme	association	1369:1379	arg1	sustainable					1413:1423	sustainable	1413:1423	sustainable	1413:1423	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	5	theme	association	1369:1379	arg1	Investigation					1218:1230	Investigation	1218:1230	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association	1218:1379	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	5	theme	association	1369:1379	arg1	green					1403:1407	green	1403:1407	green	1403:1407	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	6	6	theme	biopolymer	1200:1209	arg1	films					1211:1215	the pure biopolymer films	1191:1215	the pure biopolymer films	1191:1215	The blend films also exhibited high tensile strength (up to 24 MPa) compared to the pure biopolymer films.					
33636265	1	7	theme	blending	273:280	arg1	ratio					282:286	their optimum blending ratio	259:286	their optimum blending ratio	259:286	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	0	8	theme	flexible	100:107	arg1	films					119:123	flexible packaging films	100:123	flexible packaging films	100:123	Biomacromolecule assembly based on gum kondagogu-sodium alginate composites and their expediency in flexible packaging films.					
33636265	0	9	from	composites	65:74	arg1	films					119:123	flexible packaging films	100:123	flexible packaging films	100:123	Biomacromolecule assembly based on gum kondagogu-sodium alginate composites and their expediency in flexible packaging films.					
33636265	2	10	theme	hydroxyl	624:631	arg1	carbonyl					634:641	the hydroxyl, carbonyl, and acetyl groups	620:660	carbonyl	634:641	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	5	11	theme	water	988:992	arg1	angle					1002:1006	The water contact angle	984:1006	The water contact angle of the blend films	984:1025	The water contact angle of the blend films reached up to 81°, although the value for pure biomacromolecule films was very low.					
33636265	4	12	theme	emission	755:762	arg1	FESEM					794:798	FESEM	794:798	FESEM	794:798	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	4	12	theme	emission	755:762	arg1	microscopy					782:791	Field emission scanning electron microscopy	749:791	Field emission scanning electron microscopy (FESEM)	749:799	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	7	13	theme	strength	1268:1275	arg1	sustainable					1413:1423	sustainable	1413:1423	sustainable	1413:1423	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	13	theme	strength	1268:1275	arg1	Investigation					1218:1230	Investigation	1218:1230	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association	1218:1379	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	13	theme	strength	1268:1275	arg1	green					1403:1407	green	1403:1407	green	1403:1407	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	5	14	theme	contact	994:1000	arg1	angle					1002:1006	The water contact angle	984:1006	The water contact angle of the blend films	984:1025	The water contact angle of the blend films reached up to 81°, although the value for pure biomacromolecule films was very low.					
33636265	7	15	theme	developed	1334:1342	arg1	association					1369:1379	the developed KO/SA bio-macromolecular association	1330:1379	the developed KO/SA bio-macromolecular association	1330:1379	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	1	16	theme	alginate	191:198	arg1	assembly					130:137	The assembly	126:137	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA)	126:206	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	4	17	theme	films	865:869	arg1	surface					850:856	the surface	846:856	the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness	846:981	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	4	18	theme	surface	948:954	arg1	homogeneity					956:966	the surface homogeneity	944:966	the surface homogeneity	944:966	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	2	19	theme	ATR-FTIR	477:484	arg1	analysis					487:494	spectroscopy (ATR-FTIR) analysis	463:494	spectroscopy (ATR-FTIR) analysis	463:494	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	2	20	theme	acetyl	648:653	arg1	groups					655:660	the hydroxyl, carbonyl, and acetyl groups	620:660	groups	655:660	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	7	21	theme	mechanical	1257:1266	arg1	strength					1268:1275	mechanical strength	1257:1275	mechanical strength	1257:1275	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	22	dep	green	1403:1407	arg1	films					1440:1444	food packaging films	1425:1444	food packaging films	1425:1444	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	0	23	theme	Biomacromolecule	0:15	arg1	assembly					17:24	Biomacromolecule assembly	0:24	Biomacromolecule assembly	0:24	Biomacromolecule assembly based on gum kondagogu-sodium alginate composites and their expediency in flexible packaging films.					
33636265	4	24	theme	biopolymers	874:884	arg1	films					865:869	the films	861:869	the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness	861:981	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	4	25	theme	electron	773:780	arg1	FESEM					794:798	FESEM	794:798	FESEM	794:798	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	4	25	theme	electron	773:780	arg1	microscopy					782:791	Field emission scanning electron microscopy	749:791	Field emission scanning electron microscopy (FESEM)	749:799	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	7	26	theme	permeability	1278:1289	arg1	sustainable					1413:1423	sustainable	1413:1423	sustainable	1413:1423	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	26	theme	permeability	1278:1289	arg1	Investigation					1218:1230	Investigation	1218:1230	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association	1218:1379	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	26	theme	permeability	1278:1289	arg1	green					1403:1407	green	1403:1407	green	1403:1407	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	5	27	theme	pure	1069:1072	arg1	films					1091:1095	pure biomacromolecule films	1069:1095	pure biomacromolecule films	1069:1095	The water contact angle of the blend films reached up to 81°, although the value for pure biomacromolecule films was very low.					
33636265	2	28	theme	spectroscopy	463:474	arg1	analysis					487:494	spectroscopy (ATR-FTIR) analysis	463:494	spectroscopy (ATR-FTIR) analysis	463:494	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	7	29	theme	food	1425:1428	arg1	films					1440:1444	food packaging films	1425:1444	food packaging films	1425:1444	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	6	30	theme	pure	1195:1198	arg1	films					1211:1215	the pure biopolymer films	1191:1215	the pure biopolymer films	1191:1215	The blend films also exhibited high tensile strength (up to 24 MPa) compared to the pure biopolymer films.					
33636265	6	31	theme	high	1142:1145	arg1	strength					1155:1162	high tensile strength	1142:1162	high tensile strength (up to 24 MPa)	1142:1177	The blend films also exhibited high tensile strength (up to 24 MPa) compared to the pure biopolymer films.					
33636265	7	32	theme	transparency	1292:1303	arg1	sustainable					1413:1423	sustainable	1413:1423	sustainable	1413:1423	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	32	theme	transparency	1292:1303	arg1	Investigation					1218:1230	Investigation	1218:1230	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association	1218:1379	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	32	theme	transparency	1292:1303	arg1	green					1403:1407	green	1403:1407	green	1403:1407	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	6	33	theme	tensile	1147:1153	arg1	strength					1155:1162	high tensile strength	1142:1162	high tensile strength (up to 24 MPa)	1142:1177	The blend films also exhibited high tensile strength (up to 24 MPa) compared to the pure biopolymer films.					
33636265	4	34	theme	SA	931:932	arg1	addition					919:926	the addition	915:926	the addition of SA	915:932	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	1	35	theme	morphological	378:390	arg1	properties					392:401	physical and chemical, structural, mechanical, barrier, and morphological properties	318:401	properties	392:401	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	7	36	theme	film-forming	1235:1246	arg1	ability					1248:1254	film-forming ability	1235:1254	film-forming ability	1235:1254	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	0	37	theme	kondagogu-sodium	39:54	arg1	composites					65:74	gum kondagogu-sodium alginate composites	35:74	gum kondagogu-sodium alginate composites	35:74	Biomacromolecule assembly based on gum kondagogu-sodium alginate composites and their expediency in flexible packaging films.					
33636265	1	38	dep	structural	341:350	arg1	mechanical					353:362	mechanical	353:362	mechanical	353:362	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	2	39	theme	bond	568:571	arg1	interaction					573:583	the hydrogen bond interaction	555:583	the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups	555:660	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	0	40	from	expediency	86:95	arg1	films					119:123	flexible packaging films	100:123	flexible packaging films	100:123	Biomacromolecule assembly based on gum kondagogu-sodium alginate composites and their expediency in flexible packaging films.					
33636265	7	41	theme	biodegradability	1310:1325	arg1	sustainable					1413:1423	sustainable	1413:1423	sustainable	1413:1423	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	41	theme	biodegradability	1310:1325	arg1	Investigation					1218:1230	Investigation	1218:1230	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association	1218:1379	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	41	theme	biodegradability	1310:1325	arg1	green					1403:1407	green	1403:1407	green	1403:1407	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	2	42	theme	total	419:423	arg1	reflection-Fourier					425:442	The attenuated total reflection-Fourier	404:442	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis	404:494	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	0	43	theme	gum	35:37	arg1	composites					65:74	gum kondagogu-sodium alginate composites	35:74	gum kondagogu-sodium alginate composites	35:74	Biomacromolecule assembly based on gum kondagogu-sodium alginate composites and their expediency in flexible packaging films.					
33636265	2	44	theme	hydrogen	559:566	arg1	interaction					573:583	the hydrogen bond interaction	555:583	the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups	555:660	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	3	45	theme	Structural	663:672	arg1	identification					674:687	Structural identification	663:687	Structural identification	663:687	Structural identification was performed by recording X-ray diffraction (XRD) spectra.					
33636265	2	46	theme	attenuated	408:417	arg1	reflection-Fourier					425:442	The attenuated total reflection-Fourier	404:442	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis	404:494	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	1	47	theme	physical	318:325	arg1	barrier					365:371	physical and chemical, structural, mechanical, barrier, and morphological properties	318:401	barrier	365:371	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	2	48	dep	transform	444:452	arg1	infrared					454:461	infrared	454:461	transform infrared spectroscopy (ATR-FTIR) analysis	444:494	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	4	49	theme	Field	749:753	arg1	FESEM					794:798	FESEM	794:798	FESEM	794:798	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	4	49	theme	Field	749:753	arg1	microscopy					782:791	Field emission scanning electron microscopy	749:791	Field emission scanning electron microscopy (FESEM)	749:799	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	0	50	theme	alginate	56:63	arg1	composites					65:74	gum kondagogu-sodium alginate composites	35:74	gum kondagogu-sodium alginate composites	35:74	Biomacromolecule assembly based on gum kondagogu-sodium alginate composites and their expediency in flexible packaging films.					
33636265	3	51	theme	X-ray	716:720	arg1	XRD					735:737	XRD	735:737	XRD	735:737	Structural identification was performed by recording X-ray diffraction (XRD) spectra.					
33636265	3	51	theme	X-ray	716:720	arg1	diffraction					722:732	X-ray diffraction	716:732	X-ray diffraction (XRD) spectra	716:746	Structural identification was performed by recording X-ray diffraction (XRD) spectra.					
33636265	2	52	dep	reflection-Fourier	425:442	arg1	transform					444:452	transform	444:452	transform infrared spectroscopy (ATR-FTIR) analysis	444:494	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	5	53	theme	biomacromolecule	1074:1089	arg1	films					1091:1095	pure biomacromolecule films	1069:1095	pure biomacromolecule films	1069:1095	The water contact angle of the blend films reached up to 81°, although the value for pure biomacromolecule films was very low.					
33636265	4	54	used	used	805:808	arg2	FESEM					794:798	FESEM	794:798	FESEM	794:798	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	4	54	used	used	805:808	arg2	microscopy					782:791	Field emission scanning electron microscopy	749:791	Field emission scanning electron microscopy (FESEM)	749:799	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	1	55	theme	chemical	331:338	arg1	barrier					365:371	physical and chemical, structural, mechanical, barrier, and morphological properties	318:401	barrier	365:371	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	3	56	theme	diffraction	722:732	arg1	spectra					740:746	X-ray diffraction (XRD) spectra	716:746	X-ray diffraction (XRD) spectra	716:746	Structural identification was performed by recording X-ray diffraction (XRD) spectra.					
33636265	7	57	theme	KO/SA	1344:1348	arg1	association					1369:1379	the developed KO/SA bio-macromolecular association	1330:1379	the developed KO/SA bio-macromolecular association	1330:1379	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	58	theme	packaging	1430:1438	arg1	films					1440:1444	food packaging films	1425:1444	food packaging films	1425:1444	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	4	59	theme	scanning	764:771	arg1	FESEM					794:798	FESEM	794:798	FESEM	794:798	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	4	59	theme	scanning	764:771	arg1	microscopy					782:791	Field emission scanning electron microscopy	749:791	Field emission scanning electron microscopy (FESEM)	749:799	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	2	60	theme	molecular	593:601	arg1	chains					603:608	molecular chains	593:608	molecular chains involving the hydroxyl, carbonyl, and acetyl groups	593:660	The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that both biomacromolecules are well organised due to the hydrogen bond interaction between molecular chains involving the hydroxyl, carbonyl, and acetyl groups.					
33636265	7	61	theme	bio-macromolecular	1350:1367	arg1	association					1369:1379	the developed KO/SA bio-macromolecular association	1330:1379	the developed KO/SA bio-macromolecular association	1330:1379	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	1	62	theme	bio-based	142:150	arg1	macromolecules					152:165	bio-based macromolecules	142:165	bio-based macromolecules	142:165	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	4	63	theme	conjugates	897:906	arg1	films					865:869	the films	861:869	the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness	861:981	Field emission scanning electron microscopy (FESEM) was used to identify the distinction between the surface of the films of biopolymers, and their conjugates, where the addition of SA increased the surface homogeneity and smoothness.					
33636265	6	64	theme	blend	1115:1119	arg1	films					1121:1125	The blend films	1111:1125	The blend films	1111:1125	The blend films also exhibited high tensile strength (up to 24 MPa) compared to the pure biopolymer films.					
33636265	5	65	theme	blend	1015:1019	arg1	films					1021:1025	the blend films	1011:1025	the blend films	1011:1025	The water contact angle of the blend films reached up to 81°, although the value for pure biomacromolecule films was very low.					
33636265	1	66	theme	macromolecules	152:165	arg1	assembly					130:137	The assembly	126:137	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA)	126:206	The assembly of bio-based macromolecules of gum kondagogu/sodium alginate (KO/SA) was fabricated using glycerol as a plasticiser and their optimum blending ratio was identified based on their physical and chemical, structural, mechanical, barrier, and morphological properties.					
33636265	5	67	theme	films	1021:1025	arg1	angle					1002:1006	The water contact angle	984:1006	The water contact angle of the blend films	984:1025	The water contact angle of the blend films reached up to 81°, although the value for pure biomacromolecule films was very low.					
33636265	7	68	theme	ability	1248:1254	arg1	sustainable					1413:1423	sustainable	1413:1423	sustainable	1413:1423	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	68	theme	ability	1248:1254	arg1	Investigation					1218:1230	Investigation	1218:1230	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association	1218:1379	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
33636265	7	68	theme	ability	1248:1254	arg1	green					1403:1407	green	1403:1407	green	1403:1407	Investigation of film-forming ability, mechanical strength, permeability, transparency, and biodegradability of the developed KO/SA bio-macromolecular association may be established as green and sustainable food packaging films.					
32414448	0	0	theme	response	97:104	arg1	surface					106:112	response surface	97:112	response surface for burn repair	97:128	Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair.					
32414448	6	1	theme	commercial	996:1005	arg1	film					1007:1010	a commercial film	994:1010	a commercial film (DUO DERM)	994:1021	This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM).					
32414448	6	1	theme	commercial	996:1005	arg1	DERM					1017:1020	DUO DERM	1013:1020	DUO DERM	1013:1020	This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM).					
32414448	0	2	theme	burn	118:121	arg1	repair					123:128	burn repair	118:128	burn repair	118:128	Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair.					
32414448	6	3	theme	burn	795:798	arg1	model					800:804	a skin burn model	788:804	a skin burn model	788:804	This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM).					
32414448	5	4	theme	tensile	623:629	arg1	modulus					639:645	the tensile elastic modulus	619:645	the tensile elastic modulus of the hydrogel	619:661	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	4	theme	tensile	623:629	arg1	kPa					682:684	480.43 ± 15.82 kPa	667:684	480.43 ± 15.82 kPa	667:684	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	6	5	theme	hydrogel	832:839	arg1	dressing					841:848	the hydrogel dressing	828:848	the hydrogel dressing	828:848	This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM).					
32414448	4	6	theme	hydrogel	538:545	arg1	properties					506:515	The mechanical properties	491:515	The mechanical properties	491:515	The mechanical properties and pore size of the hydrogel were optimized by response surface methodology.					
32414448	4	6	theme	hydrogel	538:545	arg1	size					526:529	pore size	521:529	pore size	521:529	The mechanical properties and pore size of the hydrogel were optimized by response surface methodology.					
32414448	5	7	theme	55.23	710:714	arg1	%					723:723	55.23 ± 2.43 %	710:723	55.23 ± 2.43 %	710:723	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	7	theme	55.23	710:714	arg1	strain					699:704	the tensile strain	687:704	the tensile strain	687:704	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	6	8	theme	skin	790:793	arg1	model					800:804	a skin burn model	788:804	a skin burn model	788:804	This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM).					
32414448	5	9	theme	90.43	748:752	arg1	μm					761:762	90.43 ± 5.57 μm	748:762	90.43 ± 5.57 μm	748:762	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	9	theme	90.43	748:752	arg1	size					739:742	the pore size	730:742	the pore size	730:742	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	2	10	theme	burn	290:293	arg1	dressing					295:302	a promising burn dressing	278:302	a promising burn dressing	278:302	The purpose of this study was to develop a promising burn dressing.					
32414448	1	11	theme	burn	195:198	arg1	healing					206:212	burn wound healing	195:212	burn wound healing	195:212	Collagen and polysaccharide materials have great advantages for burn wound healing and skin regeneration.					
32414448	7	12	theme	burn	1089:1092	arg1	dressing					1100:1107	a promising burn wound dressing	1077:1107	a promising burn wound dressing	1077:1107	Therefore, the HLC/HA/CCS hydrogel can be considered a promising burn wound dressing.					
32414448	5	13	theme	±	716:716	arg1	%					723:723	55.23 ± 2.43 %	710:723	55.23 ± 2.43 %	710:723	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	13	theme	±	716:716	arg1	strain					699:704	the tensile strain	687:704	the tensile strain	687:704	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	2	14	theme	promising	280:288	arg1	dressing					295:302	a promising burn dressing	278:302	a promising burn dressing	278:302	The purpose of this study was to develop a promising burn dressing.					
32414448	1	15	theme	wound	200:204	arg1	healing					206:212	burn wound healing	195:212	burn wound healing	195:212	Collagen and polysaccharide materials have great advantages for burn wound healing and skin regeneration.					
32414448	1	16	contain	have	169:172	arg2	advantages					180:189	great advantages	174:189	great advantages for burn wound healing and skin regeneration	174:234	Collagen and polysaccharide materials have great advantages for burn wound healing and skin regeneration.					
32414448	1	16	contain	have	169:172	arg1	materials					159:167	Collagen and polysaccharide materials	131:167	Collagen and polysaccharide materials	131:167	Collagen and polysaccharide materials have great advantages for burn wound healing and skin regeneration.					
32414448	3	17	theme	extracellular	404:416	arg1	ECM					426:428	ECM	426:428	ECM	426:428	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	17	theme	extracellular	404:416	arg1	matrix					418:423	extracellular matrix	404:423	extracellular matrix (ECM)	404:429	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	7	18	theme	HLC/HA/CCS	1039:1048	arg1	hydrogel					1050:1057	the HLC/HA/CCS hydrogel	1035:1057	the HLC/HA/CCS hydrogel	1035:1057	Therefore, the HLC/HA/CCS hydrogel can be considered a promising burn wound dressing.					
32414448	6	19	theme	wound	975:979	arg1	healing					981:987	burn wound healing	970:987	burn wound healing	970:987	This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM).					
32414448	0	20	theme	collagen	27:34	arg1	preparation					79:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair.					
32414448	6	21	theme	bacteria	876:883	arg1	infection					885:893	bacteria infection	876:893	bacteria infection	876:893	This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM).					
32414448	5	22	theme	hydrogel	654:661	arg1	modulus					639:645	the tensile elastic modulus	619:645	the tensile elastic modulus of the hydrogel	619:661	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	22	theme	hydrogel	654:661	arg1	kPa					682:684	480.43 ± 15.82 kPa	667:684	480.43 ± 15.82 kPa	667:684	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	6	23	theme	hydrogel	918:925	arg1	dressing					927:934	the hydrogel dressing	914:934	the hydrogel dressing	914:934	This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM).					
32414448	6	23	theme	hydrogel	918:925	arg1	beneficial					945:954	beneficial	945:954	beneficial	945:954	This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM).					
32414448	1	24	theme	skin	218:221	arg1	regeneration					223:234	skin regeneration	218:234	skin regeneration	218:234	Collagen and polysaccharide materials have great advantages for burn wound healing and skin regeneration.					
32414448	0	25	theme	human-like	16:25	arg1	preparation					79:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair.					
32414448	3	26	theme	glutamine	436:444	arg1	TG					460:461	TG	460:461	TG	460:461	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	26	theme	glutamine	436:444	arg1	transaminase					446:457	glutamine transaminase	436:457	glutamine transaminase (TG)	436:462	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	26	theme	glutamine	436:444	arg1	acid					323:326	Mixing hyaluronic acid	305:326	Mixing hyaluronic acid (HA)	305:331	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	26	theme	glutamine	436:444	arg1	crosslinker					478:488	a crosslinker	476:488	a crosslinker	476:488	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	26	theme	glutamine	436:444	arg1	chitosan					347:354	carboxylated chitosan	334:354	carboxylated chitosan (CCS)	334:360	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	0	27	theme	polysaccharide	46:59	arg1	preparation					79:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair.					
32414448	4	28	theme	pore	521:524	arg1	size					526:529	pore size	521:529	pore size	521:529	The mechanical properties and pore size of the hydrogel were optimized by response surface methodology.					
32414448	5	29	theme	480.43	667:672	arg1	kPa					682:684	480.43 ± 15.82 kPa	667:684	480.43 ± 15.82 kPa	667:684	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	29	theme	480.43	667:672	arg1	modulus					639:645	the tensile elastic modulus	619:645	the tensile elastic modulus of the hydrogel	619:661	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	2	30	theme	study	257:261	arg1	purpose					241:247	The purpose	237:247	The purpose of this study	237:261	The purpose of this study was to develop a promising burn dressing.					
32414448	5	31	theme	±	754:754	arg1	μm					761:762	90.43 ± 5.57 μm	748:762	90.43 ± 5.57 μm	748:762	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	31	theme	±	754:754	arg1	size					739:742	the pore size	730:742	the pore size	730:742	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	0	32	theme	composite	36:44	arg1	preparation					79:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair.					
32414448	5	33	theme	±	674:674	arg1	kPa					682:684	480.43 ± 15.82 kPa	667:684	480.43 ± 15.82 kPa	667:684	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	33	theme	±	674:674	arg1	modulus					639:645	the tensile elastic modulus	619:645	the tensile elastic modulus of the hydrogel	619:661	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	34	theme	pore	734:737	arg1	μm					761:762	90.43 ± 5.57 μm	748:762	90.43 ± 5.57 μm	748:762	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	34	theme	pore	734:737	arg1	size					739:742	the pore size	730:742	the pore size	730:742	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	0	35	theme	dressing	70:77	arg1	preparation					79:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair.					
32414448	7	36	theme	wound	1094:1098	arg1	dressing					1100:1107	a promising burn wound dressing	1077:1107	a promising burn wound dressing	1077:1107	Therefore, the HLC/HA/CCS hydrogel can be considered a promising burn wound dressing.					
32414448	5	37	theme	elastic	631:637	arg1	modulus					639:645	the tensile elastic modulus	619:645	the tensile elastic modulus of the hydrogel	619:661	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	37	theme	elastic	631:637	arg1	kPa					682:684	480.43 ± 15.82 kPa	667:684	480.43 ± 15.82 kPa	667:684	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	3	38	theme	carboxylated	334:345	arg1	acid					323:326	Mixing hyaluronic acid	305:326	Mixing hyaluronic acid (HA)	305:331	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	38	theme	carboxylated	334:345	arg1	crosslinker					478:488	a crosslinker	476:488	a crosslinker	476:488	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	38	theme	carboxylated	334:345	arg1	CCS					357:359	CCS	357:359	CCS	357:359	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	38	theme	carboxylated	334:345	arg1	transaminase					446:457	glutamine transaminase	436:457	glutamine transaminase (TG)	436:462	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	38	theme	carboxylated	334:345	arg1	chitosan					347:354	carboxylated chitosan	334:354	carboxylated chitosan (CCS)	334:360	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	1	39	theme	Collagen	131:138	arg1	materials					159:167	Collagen and polysaccharide materials	131:167	Collagen and polysaccharide materials	131:167	Collagen and polysaccharide materials have great advantages for burn wound healing and skin regeneration.					
32414448	0	40	theme	hydrogel	61:68	arg1	preparation					79:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	human-like collagen composite polysaccharide hydrogel dressing preparation	16:89	Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair.					
32414448	4	41	theme	mechanical	495:504	arg1	properties					506:515	The mechanical properties	491:515	The mechanical properties	491:515	The mechanical properties and pore size of the hydrogel were optimized by response surface methodology.					
32414448	3	42	theme	Mixing	305:310	arg1	transaminase					446:457	glutamine transaminase	436:457	glutamine transaminase (TG)	436:462	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	42	theme	Mixing	305:310	arg1	HA					329:330	HA	329:330	HA	329:330	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	42	theme	Mixing	305:310	arg1	acid					323:326	Mixing hyaluronic acid	305:326	Mixing hyaluronic acid (HA)	305:331	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	42	theme	Mixing	305:310	arg1	crosslinker					478:488	a crosslinker	476:488	a crosslinker	476:488	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	42	theme	Mixing	305:310	arg1	chitosan					347:354	carboxylated chitosan	334:354	carboxylated chitosan (CCS)	334:360	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	43	used	used	468:471	arg2	transaminase					446:457	glutamine transaminase	436:457	glutamine transaminase (TG)	436:462	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	43	used	used	468:471	arg2	TG					460:461	TG	460:461	TG	460:461	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	43	used	used	468:471	arg2	crosslinker					478:488	a crosslinker	476:488	a crosslinker	476:488	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	43	used	used	468:471	arg2	acid					323:326	Mixing hyaluronic acid	305:326	Mixing hyaluronic acid (HA)	305:331	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	43	used	used	468:471	arg2	chitosan					347:354	carboxylated chitosan	334:354	carboxylated chitosan (CCS)	334:360	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	43	used	used	468:471	arg2	HA					329:330	HA	329:330	HA	329:330	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	43	used	used	468:471	arg2	CCS					357:359	CCS	357:359	CCS	357:359	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	1	44	theme	polysaccharide	144:157	arg1	materials					159:167	Collagen and polysaccharide materials	131:167	Collagen and polysaccharide materials	131:167	Collagen and polysaccharide materials have great advantages for burn wound healing and skin regeneration.					
32414448	3	45	theme	hyaluronic	312:321	arg1	transaminase					446:457	glutamine transaminase	436:457	glutamine transaminase (TG)	436:462	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	45	theme	hyaluronic	312:321	arg1	HA					329:330	HA	329:330	HA	329:330	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	45	theme	hyaluronic	312:321	arg1	acid					323:326	Mixing hyaluronic acid	305:326	Mixing hyaluronic acid (HA)	305:331	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	45	theme	hyaluronic	312:321	arg1	crosslinker					478:488	a crosslinker	476:488	a crosslinker	476:488	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	45	theme	hyaluronic	312:321	arg1	chitosan					347:354	carboxylated chitosan	334:354	carboxylated chitosan (CCS)	334:360	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	5	46	theme	tensile	691:697	arg1	%					723:723	55.23 ± 2.43 %	710:723	55.23 ± 2.43 %	710:723	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	5	46	theme	tensile	691:697	arg1	strain					699:704	the tensile strain	687:704	the tensile strain	687:704	The results showed that the tensile elastic modulus of the hydrogel was 480.43 ± 15.82 kPa, the tensile strain was 55.23 ± 2.43 %, and the pore size was 90.43 ± 5.57 μm.					
32414448	6	47	theme	burn	970:973	arg1	healing					981:987	burn wound healing	970:987	burn wound healing	970:987	This study constructed a skin burn model and demonstrated that the hydrogel dressing could effectively prevent bacteria infection and confirmed that the hydrogel dressing was more beneficial for promoting burn wound healing than a commercial film (DUO DERM).					
32414448	4	48	theme	surface	574:580	arg1	methodology					582:592	response surface methodology	565:592	response surface methodology	565:592	The mechanical properties and pore size of the hydrogel were optimized by response surface methodology.					
32414448	0	49	theme	preparation	79:89	arg1	Optimization					0:11	Optimization	0:11	Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair.	0:129	Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair.					
32414448	7	50	theme	promising	1079:1087	arg1	dressing					1100:1107	a promising burn wound dressing	1077:1107	a promising burn wound dressing	1077:1107	Therefore, the HLC/HA/CCS hydrogel can be considered a promising burn wound dressing.					
32414448	3	51	theme	human-like	366:375	arg1	collagen					377:384	human-like collagen	366:384	human-like collagen (HLC)	366:390	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	3	51	theme	human-like	366:375	arg1	HLC					387:389	HLC	387:389	HLC	387:389	Mixing hyaluronic acid (HA), carboxylated chitosan (CCS) and human-like collagen (HLC) to simulate extracellular matrix (ECM), and glutamine transaminase (TG) was used as a crosslinker.					
32414448	1	52	theme	great	174:178	arg1	advantages					180:189	great advantages	174:189	great advantages for burn wound healing and skin regeneration	174:234	Collagen and polysaccharide materials have great advantages for burn wound healing and skin regeneration.					
32414448	4	53	theme	response	565:572	arg1	methodology					582:592	response surface methodology	565:592	response surface methodology	565:592	The mechanical properties and pore size of the hydrogel were optimized by response surface methodology.					
32988512	4	0	theme	COS	770:772	arg1	20 mg					774:778	4) COS 20 mg	767:778	4) COS 20 mg	767:778	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	0	theme	COS	770:772	arg1	groups					668:673	6 groups	666:673	6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection	666:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	3	1	from	effects	492:498	arg1	microbiome					546:555	the cecal microbiome	536:555	the cecal microbiome	536:555	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	3	1	from	effects	492:498	arg1	pathways					568:575	metabolic pathways	558:575	metabolic pathways	558:575	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	3	1	from	effects	492:498	arg1	metabolites					595:605	fermentation metabolites	582:605	fermentation metabolites of chickens	582:617	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	6	2	theme	standard	983:990	arg1	practices					1002:1010	standard husbandry practices	983:1010	standard husbandry practices	983:1010	The hatched chicks were raised for 21 D under standard husbandry practices.					
32988512	12	3	theme	COS	1901:1903	arg1	factors					1915:1921	the COS and other factors	1897:1921	factors	1915:1921	A network based on the correlation between the COS and other factors was constructed to illuminate the potential action mechanism of the COS in chicken early nutrition.					
32988512	10	4	theme	biosynthesis	1733:1744	arg1	pathways					1637:1644	the pathways	1633:1644	the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis	1633:1744	At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group.					
32988512	11	5	theme	acid	1801:1804	arg1	content					1806:1812	propionic acid content	1791:1812	propionic acid content	1791:1812	In addition, propionic acid content was higher (P < 0.05) in the COS group.					
32988512	4	6	dep	control	719:725	arg1	1					703:703	1	703:703	1	703:703	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	0	7	theme	broiler	163:169	arg1	chickens					171:178	broiler chickens	163:178	broiler chickens	163:178	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	4	8	theme	fertile	635:641	arg1	eggs					643:646	240 fertile eggs	631:646	240 fertile eggs	631:646	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	3	9	theme	cecal	540:544	arg1	microbiome					546:555	the cecal microbiome	536:555	the cecal microbiome	536:555	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	8	10	from	groups	1249:1254	arg1	day					1259:1261	day 3	1259:1263	day 3	1259:1263	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	13	11	theme	cecal	2100:2104	arg1	microbiota					2106:2115	the cecal microbiota	2096:2115	the cecal microbiota	2096:2115	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	9	12	theme	relative	1494:1501	arg1	lower					1560:1564	lower	1560:1564	lower	1560:1564	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	9	12	theme	relative	1494:1501	arg1	abundance					1503:1511	the relative abundance	1490:1511	the relative abundance of some opportunistic pathogenic bacteria	1490:1553	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	2	13	from	benefits	366:373	arg1	health					406:411	the gut health	398:411	the gut health of chickens when used in early nutrition	398:452	However, the benefits of these prebiotics on the gut health of chickens when used in early nutrition are unknown.					
32988512	8	14	theme	microbial	1178:1186	arg1	P > 0.05					1219:1226	P > 0.05	1219:1226	P > 0.05	1219:1226	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	14	theme	microbial	1178:1186	arg1	composition					1188:1198	The cecal microbial composition	1168:1198	The cecal microbial composition	1168:1198	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	14	theme	microbial	1178:1186	arg1	different					1208:1216	different	1208:1216	different	1208:1216	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	14	theme	microbial	1178:1186	arg1	P < 0.05					1284:1291	P < 0.05	1284:1291	P < 0.05	1284:1291	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	14	theme	microbial	1178:1186	arg1	different					1273:1281	different	1273:1281	different	1273:1281	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	1	15	from	supplements	270:280	arg1	industry					297:304	the poultry industry	285:304	the poultry industry for improving growth performance and immunity	285:350	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	1	16	theme	chlorella	216:224	arg1	CPS					242:244	CPS	242:244	CPS	242:244	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	1	16	theme	chlorella	216:224	arg1	polysaccharide					226:239	chlorella polysaccharide	216:239	chlorella polysaccharide (CPS)	216:245	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	1	16	theme	chlorella	216:224	arg1	chitooligosaccharide					185:204	The chitooligosaccharide	181:204	The chitooligosaccharide (COS)	181:210	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	1	16	theme	chlorella	216:224	arg1	supplements					270:280	feed supplements	265:280	feed supplements in the poultry industry for improving growth performance and immunity	265:350	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	13	17	theme	chickens	2223:2230	arg1	health					2213:2218	the gut health	2205:2218	the gut health of chickens	2205:2230	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	13	18	theme	positive	2076:2083	arg1	effects					2085:2091	positive effects	2076:2091	positive effects on the cecal microbiota, metabolic pathways, and propionic acid	2076:2155	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	9	19	theme	opportunistic	1521:1533	arg1	bacteria					1546:1553	some opportunistic pathogenic bacteria	1516:1553	some opportunistic pathogenic bacteria	1516:1553	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	13	20	dep	in	2178:2179	arg1	ovo					2181:2183	ovo	2181:2183	ovo	2181:2183	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	4	21	dep	injection	811:819	arg1	6					798:798	6	798:798	6	798:798	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	9	22	theme	bacteria	1546:1553	arg1	lower					1560:1564	lower	1560:1564	lower	1560:1564	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	9	22	theme	bacteria	1546:1553	arg1	abundance					1503:1511	the relative abundance	1490:1511	the relative abundance of some opportunistic pathogenic bacteria	1490:1553	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	0	23	from	pathways	121:128	arg1	chickens					171:178	broiler chickens	163:178	broiler chickens	163:178	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	3	24	dep	in	503:504	arg1	ovo					506:508	ovo	506:508	ovo	506:508	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	4	25	dep	=	678:678	arg1	eggs/replicate					686:699	4; 10 eggs/replicate	680:699	4; 10 eggs/replicate	680:699	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	26	theme	20 mg	805:809	arg1	groups					668:673	6 groups	666:673	6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection	666:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	26	theme	20 mg	805:809	arg1	injection					811:819	6) CPS 20 mg injection	798:819	6) CPS 20 mg injection	798:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	27	dep	20 mg	774:778	arg1	4					767:767	4	767:767	4	767:767	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	10	28	theme	functional	1615:1624	arg1	level					1626:1630	the functional level	1611:1630	the functional level	1611:1630	At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group.					
32988512	1	29	theme	poultry	289:295	arg1	industry					297:304	the poultry industry	285:304	the poultry industry for improving growth performance and immunity	285:350	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	0	30	theme	microbial	90:98	arg1	community					100:108	cecal microbial community	84:108	cecal microbial community	84:108	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	9	31	theme	species	1312:1318	arg1	level					1320:1324	the species level	1308:1324	the species level	1308:1324	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	12	32	theme	chicken	1998:2004	arg1	nutrition					2012:2020	chicken early nutrition	1998:2020	chicken early nutrition	1998:2020	A network based on the correlation between the COS and other factors was constructed to illuminate the potential action mechanism of the COS in chicken early nutrition.					
32988512	5	33	theme	eggs	906:909	arg1	sac					899:901	the amniotic sac	886:901	the amniotic sac of eggs in respective treatments	886:934	On day 12.5 of egg incubation, test substrate was injected into the amniotic sac of eggs in respective treatments.					
32988512	8	34	theme	cecal	1172:1176	arg1	P > 0.05					1219:1226	P > 0.05	1219:1226	P > 0.05	1219:1226	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	34	theme	cecal	1172:1176	arg1	composition					1188:1198	The cecal microbial composition	1168:1198	The cecal microbial composition	1168:1198	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	34	theme	cecal	1172:1176	arg1	different					1208:1216	different	1208:1216	different	1208:1216	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	34	theme	cecal	1172:1176	arg1	P < 0.05					1284:1291	P < 0.05	1284:1291	P < 0.05	1284:1291	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	34	theme	cecal	1172:1176	arg1	different					1273:1281	different	1273:1281	different	1273:1281	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	5	35	theme	respective	914:923	arg1	treatments					925:934	respective treatments	914:934	respective treatments	914:934	On day 12.5 of egg incubation, test substrate was injected into the amniotic sac of eggs in respective treatments.					
32988512	9	36	theme	polysaccharide-utilizing	1331:1354	arg1	coprocola					1412:1420	Bacteroides coprocola	1400:1420	Bacteroides coprocola	1400:1420	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	9	36	theme	polysaccharide-utilizing	1331:1354	arg1	higher					1457:1462	higher	1457:1462	higher	1457:1462	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	9	36	theme	polysaccharide-utilizing	1331:1354	arg1	salanitronis					1439:1450	Bacteroides salanitronis	1427:1450	Bacteroides salanitronis	1427:1450	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	9	36	theme	polysaccharide-utilizing	1331:1354	arg1	bacteria					1356:1363	the polysaccharide-utilizing bacteria	1327:1363	the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis	1327:1450	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	9	36	theme	polysaccharide-utilizing	1331:1354	arg1	johnsonii					1389:1397	Lactobacillus johnsonii	1375:1397	Lactobacillus johnsonii	1375:1397	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	7	37	theme	gas	1148:1150	arg1	chromatography					1152:1165	gas chromatography	1148:1165	gas chromatography	1148:1165	On day 3 and 21, cecal digesta were collected to determine microbiota by shotgun metagenomic sequencing and short-chain fatty acids by gas chromatography.					
32988512	2	38	theme	early	438:442	arg1	nutrition					444:452	early nutrition	438:452	early nutrition	438:452	However, the benefits of these prebiotics on the gut health of chickens when used in early nutrition are unknown.					
32988512	2	39	theme	prebiotics	384:393	arg1	unknown					458:464	unknown	458:464	unknown	458:464	However, the benefits of these prebiotics on the gut health of chickens when used in early nutrition are unknown.					
32988512	2	39	theme	prebiotics	384:393	arg1	benefits					366:373	the benefits	362:373	the benefits of these prebiotics on the gut health of chickens when used in early nutrition	362:452	However, the benefits of these prebiotics on the gut health of chickens when used in early nutrition are unknown.					
32988512	0	40	theme	In	0:1	arg1	supplementation					7:21	In ovo supplementation	0:21	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide	0:74	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	12	41	theme	COS	1991:1993	arg1	mechanism					1974:1982	the potential action mechanism	1953:1982	the potential action mechanism of the COS in chicken early nutrition	1953:2020	A network based on the correlation between the COS and other factors was constructed to illuminate the potential action mechanism of the COS in chicken early nutrition.					
32988512	2	42	theme	chickens	416:423	arg1	health					406:411	the gut health	398:411	the gut health of chickens when used in early nutrition	398:452	However, the benefits of these prebiotics on the gut health of chickens when used in early nutrition are unknown.					
32988512	4	43	dep	control	745:751	arg1	2					728:728	2	728:728	2	728:728	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	8	44	dep	different	1273:1281	arg1	P > 0.05					1219:1226	P > 0.05	1219:1226	P > 0.05	1219:1226	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	44	dep	different	1273:1281	arg1	composition					1188:1198	The cecal microbial composition	1168:1198	The cecal microbial composition	1168:1198	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	44	dep	different	1273:1281	arg1	different					1273:1281	different	1273:1281	different	1273:1281	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	44	dep	different	1273:1281	arg1	different					1208:1216	different	1208:1216	different	1208:1216	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	44	dep	different	1273:1281	arg1	P < 0.05					1284:1291	P < 0.05	1284:1291	P < 0.05	1284:1291	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	11	45	theme	propionic	1791:1799	arg1	content					1806:1812	propionic acid content	1791:1812	propionic acid content	1791:1812	In addition, propionic acid content was higher (P < 0.05) in the COS group.					
32988512	7	46	theme	short-chain	1121:1131	arg1	acids					1139:1143	short-chain fatty acids	1121:1143	short-chain fatty acids	1121:1143	On day 3 and 21, cecal digesta were collected to determine microbiota by shotgun metagenomic sequencing and short-chain fatty acids by gas chromatography.					
32988512	10	47	theme	gluconeogenesis	1649:1663	arg1	pathways					1637:1644	the pathways	1633:1644	the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis	1633:1744	At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group.					
32988512	0	48	theme	chitooligosaccharide	26:45	arg1	supplementation					7:21	In ovo supplementation	0:21	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide	0:74	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	5	49	theme	incubation	841:850	arg1	day					825:827	day 12.5	825:832	day 12.5 of egg incubation	825:850	On day 12.5 of egg incubation, test substrate was injected into the amniotic sac of eggs in respective treatments.					
32988512	3	50	theme	chickens	610:617	arg1	microbiome					546:555	the cecal microbiome	536:555	the cecal microbiome	536:555	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	3	50	theme	chickens	610:617	arg1	pathways					568:575	metabolic pathways	558:575	metabolic pathways	558:575	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	3	50	theme	chickens	610:617	arg1	metabolites					595:605	fermentation metabolites	582:605	fermentation metabolites of chickens	582:617	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	4	51	theme	saline	738:743	arg1	groups					668:673	6 groups	666:673	6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection	666:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	51	theme	saline	738:743	arg1	control					745:751	2) normal saline control	728:751	2) normal saline control	728:751	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	0	52	theme	chlorella	51:59	arg1	polysaccharide					61:74	chlorella polysaccharide	51:74	chlorella polysaccharide	51:74	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	1	53	theme	growth	320:325	arg1	performance					327:337	growth performance	320:337	growth performance	320:337	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	5	54	theme	test	853:856	arg1	substrate					858:866	test substrate	853:866	test substrate	853:866	On day 12.5 of egg incubation, test substrate was injected into the amniotic sac of eggs in respective treatments.					
32988512	7	55	theme	shotgun	1086:1092	arg1	sequencing					1106:1115	shotgun metagenomic sequencing	1086:1115	shotgun metagenomic sequencing	1086:1115	On day 3 and 21, cecal digesta were collected to determine microbiota by shotgun metagenomic sequencing and short-chain fatty acids by gas chromatography.					
32988512	4	56	theme	no-injection	706:717	arg1	control					719:725	1) no-injection control	703:725	1) no-injection control	703:725	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	56	theme	no-injection	706:717	arg1	groups					668:673	6 groups	666:673	6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection	666:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	0	57	from	metabolites	148:158	arg1	chickens					171:178	broiler chickens	163:178	broiler chickens	163:178	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	10	58	theme	L-isoleucine	1666:1677	arg1	degradation					1679:1689	L-isoleucine degradation	1666:1689	L-isoleucine degradation	1666:1689	At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group.					
32988512	4	59	dep	5 mg	788:791	arg1	5					781:781	5	781:781	5	781:781	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	60	dep	5 mg	761:764	arg1	3					754:754	3	754:754	3	754:754	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	0	61	dep	In	0:1	arg1	ovo					3:5	ovo	3:5	ovo	3:5	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	4	62	theme	COS	757:759	arg1	5 mg					761:764	3) COS 5 mg	754:764	3) COS 5 mg	754:764	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	62	theme	COS	757:759	arg1	groups					668:673	6 groups	666:673	6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection	666:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	13	63	theme	gut	2209:2211	arg1	health					2213:2218	the gut health	2205:2218	the gut health of chickens	2205:2230	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	10	64	theme	biosynthesis	1704:1715	arg1	pathways					1637:1644	the pathways	1633:1644	the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis	1633:1744	At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group.					
32988512	12	65	theme	action	1967:1972	arg1	mechanism					1974:1982	the potential action mechanism	1953:1982	the potential action mechanism of the COS in chicken early nutrition	1953:2020	A network based on the correlation between the COS and other factors was constructed to illuminate the potential action mechanism of the COS in chicken early nutrition.					
32988512	3	66	theme	COS	521:523	arg1	feeding					510:516	in ovo feeding	503:516	in ovo feeding of COS and CPS	503:531	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	1	67	theme	feed	265:268	arg1	chitooligosaccharide					185:204	The chitooligosaccharide	181:204	The chitooligosaccharide (COS)	181:210	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	1	67	theme	feed	265:268	arg1	polysaccharide					226:239	chlorella polysaccharide	216:239	chlorella polysaccharide (CPS)	216:245	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	1	67	theme	feed	265:268	arg1	supplements					270:280	feed supplements	265:280	feed supplements in the poultry industry for improving growth performance and immunity	265:350	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	9	68	theme	COS	1471:1473	arg1	group					1475:1479	the COS group	1467:1479	the COS group	1467:1479	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	6	69	theme	husbandry	992:1000	arg1	practices					1002:1010	standard husbandry practices	983:1010	standard husbandry practices	983:1010	The hatched chicks were raised for 21 D under standard husbandry practices.					
32988512	3	70	theme	feeding	510:516	arg1	effects					492:498	the effects	488:498	the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens	488:617	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	12	71	theme	other	1909:1913	arg1	factors					1915:1921	the COS and other factors	1897:1921	factors	1915:1921	A network based on the correlation between the COS and other factors was constructed to illuminate the potential action mechanism of the COS in chicken early nutrition.					
32988512	10	72	theme	acid	1728:1731	arg1	biosynthesis					1733:1744	fatty acid biosynthesis	1722:1744	fatty acid biosynthesis	1722:1744	At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group.					
32988512	10	73	theme	COS	1767:1769	arg1	group					1771:1775	the COS group	1763:1775	the COS group	1763:1775	At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group.					
32988512	0	74	theme	fermentation	135:146	arg1	metabolites					148:158	fermentation metabolites	135:158	fermentation metabolites	135:158	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	12	75	theme	potential	1957:1965	arg1	mechanism					1974:1982	the potential action mechanism	1953:1982	the potential action mechanism of the COS in chicken early nutrition	1953:2020	A network based on the correlation between the COS and other factors was constructed to illuminate the potential action mechanism of the COS in chicken early nutrition.					
32988512	3	76	theme	CPS	529:531	arg1	feeding					510:516	in ovo feeding	503:516	in ovo feeding of COS and CPS	503:531	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	13	77	theme	COS	2060:2062	arg1	5 mg					2064:2067	COS 5 mg	2060:2067	COS 5 mg	2060:2067	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	0	78	from	community	100:108	arg1	chickens					171:178	broiler chickens	163:178	broiler chickens	163:178	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	8	79	dep	different	1208:1216	arg1	P > 0.05					1219:1226	P > 0.05	1219:1226	P > 0.05	1219:1226	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	79	dep	different	1208:1216	arg1	composition					1188:1198	The cecal microbial composition	1168:1198	The cecal microbial composition	1168:1198	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	79	dep	different	1208:1216	arg1	different					1208:1216	different	1208:1216	different	1208:1216	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	79	dep	different	1208:1216	arg1	P < 0.05					1284:1291	P < 0.05	1284:1291	P < 0.05	1284:1291	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	8	79	dep	different	1208:1216	arg1	different					1273:1281	different	1273:1281	different	1273:1281	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	6	80	theme	hatched	941:947	arg1	chicks					949:954	The hatched chicks	937:954	The hatched chicks	937:954	The hatched chicks were raised for 21 D under standard husbandry practices.					
32988512	5	81	theme	amniotic	890:897	arg1	sac					899:901	the amniotic sac	886:901	the amniotic sac of eggs in respective treatments	886:934	On day 12.5 of egg incubation, test substrate was injected into the amniotic sac of eggs in respective treatments.					
32988512	3	82	theme	metabolic	558:566	arg1	pathways					568:575	metabolic pathways	558:575	metabolic pathways	558:575	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	12	83	from	mechanism	1974:1982	arg1	nutrition					2012:2020	chicken early nutrition	1998:2020	chicken early nutrition	1998:2020	A network based on the correlation between the COS and other factors was constructed to illuminate the potential action mechanism of the COS in chicken early nutrition.					
32988512	4	84	theme	CPS	784:786	arg1	groups					668:673	6 groups	666:673	6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection	666:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	84	theme	CPS	784:786	arg1	5 mg					788:791	5) CPS 5 mg	781:791	5) CPS 5 mg	781:791	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	5	85	from	sac	899:901	arg1	treatments					925:934	respective treatments	914:934	respective treatments	914:934	On day 12.5 of egg incubation, test substrate was injected into the amniotic sac of eggs in respective treatments.					
32988512	13	86	theme	in	2038:2039	arg1	inoculation					2045:2055	in ovo inoculation	2038:2055	in ovo inoculation of COS 5 mg	2038:2067	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	9	87	theme	pathogenic	1535:1544	arg1	bacteria					1546:1553	some opportunistic pathogenic bacteria	1516:1553	some opportunistic pathogenic bacteria	1516:1553	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	13	88	dep	showed	2069:2074	arg1	used					2170:2173	used	2170:2173	can be used as in ovo feeding to modulate the gut health of chickens	2163:2230	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	3	89	theme	fermentation	582:593	arg1	metabolites					595:605	fermentation metabolites	582:605	fermentation metabolites of chickens	582:617	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	0	90	theme	cecal	84:88	arg1	community					100:108	cecal microbial community	84:108	cecal microbial community	84:108	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	11	91	theme	COS	1843:1845	arg1	group					1847:1851	the COS group	1839:1851	the COS group	1839:1851	In addition, propionic acid content was higher (P < 0.05) in the COS group.					
32988512	11	92	dep	higher	1818:1823	arg1	P < 0.05					1826:1833	P < 0.05	1826:1833	P < 0.05	1826:1833	In addition, propionic acid content was higher (P < 0.05) in the COS group.					
32988512	4	93	theme	CPS	801:803	arg1	groups					668:673	6 groups	666:673	6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection	666:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	93	theme	CPS	801:803	arg1	injection					811:819	6) CPS 20 mg injection	798:819	6) CPS 20 mg injection	798:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	94	theme	eggs	643:646	arg1	total					622:626	A total	620:626	A total of 240 fertile eggs	620:646	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	0	95	theme	metabolic	111:119	arg1	pathways					121:128	metabolic pathways	111:128	metabolic pathways	111:128	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	9	96	theme	CPS	1584:1586	arg1	groups					1600:1605	the CPS and control groups	1580:1605	groups	1600:1605	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	13	97	theme	5 mg	2064:2067	arg1	inoculation					2045:2055	in ovo inoculation	2038:2055	in ovo inoculation of COS 5 mg	2038:2067	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	13	98	theme	in	2178:2179	arg1	feeding					2185:2191	in ovo feeding	2178:2191	in ovo feeding	2178:2191	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	9	99	theme	control	1592:1598	arg1	groups					1600:1605	the CPS and control groups	1580:1605	groups	1600:1605	At the species level, the polysaccharide-utilizing bacteria including Lactobacillus johnsonii, Bacteroides coprocola, and Bacteroides salanitronis were higher in the COS group, whereas the relative abundance of some opportunistic pathogenic bacteria were lower than those in the CPS and control groups.					
32988512	12	100	theme	early	2006:2010	arg1	nutrition					2012:2020	chicken early nutrition	1998:2020	chicken early nutrition	1998:2020	A network based on the correlation between the COS and other factors was constructed to illuminate the potential action mechanism of the COS in chicken early nutrition.					
32988512	7	101	theme	fatty	1133:1137	arg1	acids					1139:1143	short-chain fatty acids	1121:1143	short-chain fatty acids	1121:1143	On day 3 and 21, cecal digesta were collected to determine microbiota by shotgun metagenomic sequencing and short-chain fatty acids by gas chromatography.					
32988512	4	102	theme	=	678:678	arg1	n					676:676	n	676:676	n = 4; 10 eggs/replicate	676:699	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	103	dep	groups	668:673	arg1	control					719:725	1) no-injection control	703:725	1) no-injection control	703:725	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	103	dep	groups	668:673	arg1	groups					668:673	6 groups	666:673	6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection	666:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	103	dep	groups	668:673	arg1	control					745:751	2) normal saline control	728:751	2) normal saline control	728:751	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	103	dep	groups	668:673	arg1	n					676:676	n	676:676	n = 4; 10 eggs/replicate	676:699	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	103	dep	groups	668:673	arg1	5 mg					788:791	5) CPS 5 mg	781:791	5) CPS 5 mg	781:791	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	103	dep	groups	668:673	arg1	injection					811:819	6) CPS 20 mg injection	798:819	6) CPS 20 mg injection	798:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	103	dep	groups	668:673	arg1	20 mg					774:778	4) COS 20 mg	767:778	4) COS 20 mg	767:778	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	103	dep	groups	668:673	arg1	5 mg					761:764	3) COS 5 mg	754:764	3) COS 5 mg	754:764	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	13	104	dep	in	2038:2039	arg1	ovo					2041:2043	ovo	2041:2043	ovo	2041:2043	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	3	105	theme	in	503:504	arg1	feeding					510:516	in ovo feeding	503:516	in ovo feeding of COS and CPS	503:531	This study evaluated the effects of in ovo feeding of COS and CPS on the cecal microbiome, metabolic pathways, and fermentation metabolites of chickens.					
32988512	5	106	theme	egg	837:839	arg1	incubation					841:850	egg incubation	837:850	egg incubation	837:850	On day 12.5 of egg incubation, test substrate was injected into the amniotic sac of eggs in respective treatments.					
32988512	7	107	theme	metagenomic	1094:1104	arg1	sequencing					1106:1115	shotgun metagenomic sequencing	1086:1115	shotgun metagenomic sequencing	1086:1115	On day 3 and 21, cecal digesta were collected to determine microbiota by shotgun metagenomic sequencing and short-chain fatty acids by gas chromatography.					
32988512	2	108	theme	gut	402:404	arg1	health					406:411	the gut health	398:411	the gut health of chickens when used in early nutrition	398:452	However, the benefits of these prebiotics on the gut health of chickens when used in early nutrition are unknown.					
32988512	0	109	theme	polysaccharide	61:74	arg1	supplementation					7:21	In ovo supplementation	0:21	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide	0:74	In ovo supplementation of chitooligosaccharide and chlorella polysaccharide affects cecal microbial community, metabolic pathways, and fermentation metabolites in broiler chickens.					
32988512	1	110	used	used	257:260	arg2	supplements					270:280	feed supplements	265:280	feed supplements in the poultry industry for improving growth performance and immunity	265:350	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	1	110	used	used	257:260	arg2	chitooligosaccharide					185:204	The chitooligosaccharide	181:204	The chitooligosaccharide (COS)	181:210	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	1	110	used	used	257:260	arg2	polysaccharide					226:239	chlorella polysaccharide	216:239	chlorella polysaccharide (CPS)	216:245	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	1	110	used	used	257:260	arg2	CPS					242:244	CPS	242:244	CPS	242:244	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	1	110	used	used	257:260	arg2	COS					207:209	COS	207:209	COS	207:209	The chitooligosaccharide (COS) and chlorella polysaccharide (CPS) have been used as feed supplements in the poultry industry for improving growth performance and immunity.					
32988512	13	111	theme	propionic	2142:2150	arg1	acid					2152:2155	propionic acid	2142:2155	propionic acid	2142:2155	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	10	112	theme	L-histidine	1692:1702	arg1	biosynthesis					1704:1715	L-histidine biosynthesis	1692:1715	L-histidine biosynthesis	1692:1715	At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group.					
32988512	4	113	theme	normal	731:736	arg1	groups					668:673	6 groups	666:673	6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection	666:819	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	4	113	theme	normal	731:736	arg1	control					745:751	2) normal saline control	728:751	2) normal saline control	728:751	A total of 240 fertile eggs were divided into 6 groups (n = 4; 10 eggs/replicate): 1) no-injection control, 2) normal saline control, 3) COS 5 mg, 4) COS 20 mg, 5) CPS 5 mg, and 6) CPS 20 mg injection.					
32988512	10	114	theme	degradation	1679:1689	arg1	pathways					1637:1644	the pathways	1633:1644	the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis	1633:1744	At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group.					
32988512	13	115	theme	metabolic	2118:2126	arg1	pathways					2128:2135	metabolic pathways	2118:2135	metabolic pathways	2118:2135	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	7	116	theme	cecal	1030:1034	arg1	digesta					1036:1042	cecal digesta	1030:1042	cecal digesta	1030:1042	On day 3 and 21, cecal digesta were collected to determine microbiota by shotgun metagenomic sequencing and short-chain fatty acids by gas chromatography.					
32988512	10	117	theme	fatty	1722:1726	arg1	biosynthesis					1733:1744	fatty acid biosynthesis	1722:1744	fatty acid biosynthesis	1722:1744	At the functional level, the pathways of gluconeogenesis, L-isoleucine degradation, L-histidine biosynthesis, and fatty acid biosynthesis were enriched in the COS group.					
32988512	8	118	theme	treatment	1239:1247	arg1	groups					1249:1254	the treatment groups	1235:1254	the treatment groups on day 3	1235:1263	The cecal microbial composition was not different (P > 0.05) among the treatment groups on day 3 but was different (P < 0.05) on day 21.					
32988512	13	119	from	effects	2085:2091	arg1	microbiota					2106:2115	the cecal microbiota	2096:2115	the cecal microbiota	2096:2115	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	13	119	from	effects	2085:2091	arg1	pathways					2128:2135	metabolic pathways	2118:2135	metabolic pathways	2118:2135	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32988512	13	119	from	effects	2085:2091	arg1	acid					2152:2155	propionic acid	2142:2155	propionic acid	2142:2155	In conclusion, in ovo inoculation of COS 5 mg showed positive effects on the cecal microbiota, metabolic pathways, and propionic acid, thus can be used as in ovo feeding to modulate the gut health of chickens.					
32535321	6	0	theme	enhanced	1046:1053	arg1	capacities					1084:1093	the pinocytic and phagocytic capacities	1055:1093	enhanced the pinocytic and phagocytic capacities of THP-1 cells	1046:1108	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	5	1	theme	-α-l-Rhap-	849:858	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	6	2	theme	mRNA	973:976	arg1	levels					1001:1006	the mRNA and protein expression levels	969:1006	the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells	969:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	5	3	dep	-α-l-Rhap-	868:877	arg1	1 → 					879:882	1 → 	879:882	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	0	4	theme	THP-1	80:84	arg1	cells					86:90	THP-1 cells	80:90	THP-1 cells	80:90	Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression.					
32535321	1	5	theme	studies	310:316	arg1	none					298:301	none	298:301	none of the studies	298:316	Huangshui (HS), the by-product of Chinese Baijiu, has attracted considerable attention due to its nutrient and microbial composition; however, none of the studies has explored the polysaccharides in HS yet.					
32535321	5	6	theme	1 → 3,6	824:830	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	6	7	from	IL-6	1021:1024	arg1	cells					1035:1039	THP-1 cells	1029:1039	THP-1 cells	1029:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	0	8	theme	NO	100:101	arg1	production					103:112	NO production	100:112	NO production	100:112	Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression.					
32535321	5	9	theme	1 → 3,4	860:866	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	6	10	theme	immunomodulatory	1132:1147	arg1	properties					1149:1158	significant immunomodulatory properties	1120:1158	significant immunomodulatory properties of HSP-3	1120:1167	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	2	11	theme	26.40 kDa	456:464	arg1	weight					446:451	an average molecular weight	425:451	an average molecular weight of 26.40 kDa	425:464	Here, from HS, we isolated a novel polysaccharide, HSP-3, with an average molecular weight of 26.40 kDa.					
32535321	6	12	theme	IL-6	1021:1024	arg1	levels					1001:1006	the mRNA and protein expression levels	969:1006	the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells	969:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	13	theme	THP-1	1029:1033	arg1	cells					1035:1039	THP-1 cells	1029:1039	THP-1 cells	1029:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	5	14	theme	-β-d-Galp-	832:841	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	7	15	theme	active	1233:1238	arg1	component					1240:1248	an active component	1230:1248	an active component in functional foods	1230:1268	Altogether, this study suggests that HSP-3 could be used as an active component in functional foods.					
32535321	7	15	theme	active	1233:1238	arg1	HSP-3					1207:1211	HSP-3	1207:1211	HSP-3	1207:1211	Altogether, this study suggests that HSP-3 could be used as an active component in functional foods.					
32535321	6	16	theme	IL-6	947:950	arg1	production					914:923	the production	910:923	the production of ROS, NO, TNF-α and IL-6	910:950	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	5	17	theme	HSP-3	752:756	arg1	backbone					740:747	The backbone	736:747	The backbone of HSP-3	736:756	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	2	18	theme	novel	391:395	arg1	HSP-3					413:417	HSP-3	413:417	HSP-3	413:417	Here, from HS, we isolated a novel polysaccharide, HSP-3, with an average molecular weight of 26.40 kDa.					
32535321	2	18	theme	novel	391:395	arg1	polysaccharide					397:410	a novel polysaccharide	389:410	a novel polysaccharide	389:410	Here, from HS, we isolated a novel polysaccharide, HSP-3, with an average molecular weight of 26.40 kDa.					
32535321	5	19	theme	1 → 4	843:847	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	6	20	theme	pinocytic	1059:1067	arg1	capacities					1084:1093	the pinocytic and phagocytic capacities	1055:1093	enhanced the pinocytic and phagocytic capacities of THP-1 cells	1046:1108	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	3	21	theme	monosaccharide	505:518	arg1	composition					520:530	monosaccharide composition	505:530	monosaccharide composition	505:530	The structure was elucidated based on monosaccharide composition and methylation analysis, NMR, FT-IR, and AFM analysis.					
32535321	0	22	theme	Immunomodulatory	0:15	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity of a novel polysaccharide	0:50	Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression.					
32535321	6	23	from	TNF-α	1011:1015	arg1	cells					1035:1039	THP-1 cells	1029:1039	THP-1 cells	1029:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	24	theme	TNF-α	937:941	arg1	production					914:923	the production	910:923	the production of ROS, NO, TNF-α and IL-6	910:950	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	25	theme	cells	1104:1108	arg1	capacities					1084:1093	the pinocytic and phagocytic capacities	1055:1093	enhanced the pinocytic and phagocytic capacities of THP-1 cells	1046:1108	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	25	theme	cells	1104:1108	arg1	upregulation					953:964	upregulation	953:964	upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells	953:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	25	theme	cells	1104:1108	arg1	stimulation					895:905	stimulation	895:905	stimulation of the production of ROS, NO, TNF-α and IL-6	895:950	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	0	26	theme	IL-6	128:131	arg1	expression					143:152	increased IL-6 and TNF-α expression	118:152	increased IL-6 and TNF-α expression	118:152	Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression.					
32535321	5	27	theme	1 → 2,6	788:794	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	6	28	from	cells	1035:1039	arg1	levels					1001:1006	the mRNA and protein expression levels	969:1006	the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells	969:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	29	theme	THP-1	1098:1102	arg1	cells					1104:1108	THP-1 cells	1098:1108	THP-1 cells	1098:1108	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	1	30	theme	microbial	266:274	arg1	composition					276:286	microbial composition	266:286	microbial composition	266:286	Huangshui (HS), the by-product of Chinese Baijiu, has attracted considerable attention due to its nutrient and microbial composition; however, none of the studies has explored the polysaccharides in HS yet.					
32535321	0	31	theme	increased	118:126	arg1	expression					143:152	increased IL-6 and TNF-α expression	118:152	increased IL-6 and TNF-α expression	118:152	Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression.					
32535321	0	32	theme	polysaccharide	37:50	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity of a novel polysaccharide	0:50	Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression.					
32535321	7	33	used	used	1222:1225	arg2	HSP-3					1207:1211	HSP-3	1207:1211	HSP-3	1207:1211	Altogether, this study suggests that HSP-3 could be used as an active component in functional foods.					
32535321	7	33	used	used	1222:1225	arg2	component					1240:1248	an active component	1230:1248	an active component in functional foods	1230:1268	Altogether, this study suggests that HSP-3 could be used as an active component in functional foods.					
32535321	0	34	theme	TNF-α	137:141	arg1	expression					143:152	increased IL-6 and TNF-α expression	118:152	increased IL-6 and TNF-α expression	118:152	Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression.					
32535321	5	35	theme	-β-d-Galp-	813:822	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	6	36	theme	significant	1120:1130	arg1	properties					1149:1158	significant immunomodulatory properties	1120:1158	significant immunomodulatory properties of HSP-3	1120:1167	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	1	37	theme	Chinese	189:195	arg1	Baijiu					197:202	Chinese Baijiu	189:202	Chinese Baijiu	189:202	Huangshui (HS), the by-product of Chinese Baijiu, has attracted considerable attention due to its nutrient and microbial composition; however, none of the studies has explored the polysaccharides in HS yet.					
32535321	0	38	theme	novel	31:35	arg1	polysaccharide					37:50	a novel polysaccharide	29:50	a novel polysaccharide	29:50	Immunomodulatory activity of a novel polysaccharide extracted from Huangshui on THP-1 cells through NO production and increased IL-6 and TNF-α expression.					
32535321	6	39	theme	TNF-α	1011:1015	arg1	levels					1001:1006	the mRNA and protein expression levels	969:1006	the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells	969:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	5	40	theme	-β-d-Manp-	777:786	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	1	41	theme	Baijiu	197:202	arg1	Huangshui					155:163	Huangshui	155:163	Huangshui (HS)	155:168	Huangshui (HS), the by-product of Chinese Baijiu, has attracted considerable attention due to its nutrient and microbial composition; however, none of the studies has explored the polysaccharides in HS yet.					
32535321	1	41	theme	Baijiu	197:202	arg1	by-product					175:184	the by-product	171:184	the by-product of Chinese Baijiu	171:202	Huangshui (HS), the by-product of Chinese Baijiu, has attracted considerable attention due to its nutrient and microbial composition; however, none of the studies has explored the polysaccharides in HS yet.					
32535321	6	42	theme	phagocytic	1073:1082	arg1	capacities					1084:1093	the pinocytic and phagocytic capacities	1055:1093	enhanced the pinocytic and phagocytic capacities of THP-1 cells	1046:1108	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	43	theme	levels	1001:1006	arg1	capacities					1084:1093	the pinocytic and phagocytic capacities	1055:1093	enhanced the pinocytic and phagocytic capacities of THP-1 cells	1046:1108	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	43	theme	levels	1001:1006	arg1	upregulation					953:964	upregulation	953:964	upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells	953:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	43	theme	levels	1001:1006	arg1	stimulation					895:905	stimulation	895:905	stimulation of the production of ROS, NO, TNF-α and IL-6	895:950	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	3	44	theme	AFM	574:576	arg1	analysis					578:585	AFM analysis	574:585	AFM analysis	574:585	The structure was elucidated based on monosaccharide composition and methylation analysis, NMR, FT-IR, and AFM analysis.					
32535321	6	45	from	levels	1001:1006	arg1	cells					1035:1039	THP-1 cells	1029:1039	THP-1 cells	1029:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	46	theme	expression	990:999	arg1	levels					1001:1006	the mRNA and protein expression levels	969:1006	the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells	969:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	3	47	theme	methylation	536:546	arg1	analysis					548:555	methylation analysis	536:555	methylation analysis	536:555	The structure was elucidated based on monosaccharide composition and methylation analysis, NMR, FT-IR, and AFM analysis.					
32535321	6	48	theme	ROS	928:930	arg1	production					914:923	the production	910:923	the production of ROS, NO, TNF-α and IL-6	910:950	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	49	theme	protein	982:988	arg1	expression					990:999	protein expression	982:999	protein expression	982:999	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	5	50	theme	-β-d-Manp-	796:805	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	6	51	theme	NO	933:934	arg1	production					914:923	the production	910:923	the production of ROS, NO, TNF-α and IL-6	910:950	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	52	theme	production	914:923	arg1	capacities					1084:1093	the pinocytic and phagocytic capacities	1055:1093	enhanced the pinocytic and phagocytic capacities of THP-1 cells	1046:1108	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	52	theme	production	914:923	arg1	upregulation					953:964	upregulation	953:964	upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells	953:1039	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	6	52	theme	production	914:923	arg1	stimulation					895:905	stimulation	895:905	stimulation of the production of ROS, NO, TNF-α and IL-6	895:950	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	2	53	theme	molecular	436:444	arg1	weight					446:451	an average molecular weight	425:451	an average molecular weight of 26.40 kDa	425:464	Here, from HS, we isolated a novel polysaccharide, HSP-3, with an average molecular weight of 26.40 kDa.					
32535321	5	54	theme	1 → 6	807:811	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	7	55	from	component	1240:1248	arg1	foods					1264:1268	functional foods	1253:1268	functional foods	1253:1268	Altogether, this study suggests that HSP-3 could be used as an active component in functional foods.					
32535321	2	56	with	polysaccharide	397:410	arg1	weight					446:451	an average molecular weight	425:451	an average molecular weight of 26.40 kDa	425:464	Here, from HS, we isolated a novel polysaccharide, HSP-3, with an average molecular weight of 26.40 kDa.					
32535321	7	57	theme	functional	1253:1262	arg1	foods					1264:1268	functional foods	1253:1268	functional foods	1253:1268	Altogether, this study suggests that HSP-3 could be used as an active component in functional foods.					
32535321	2	58	theme	average	428:434	arg1	weight					446:451	an average molecular weight	425:451	an average molecular weight of 26.40 kDa	425:464	Here, from HS, we isolated a novel polysaccharide, HSP-3, with an average molecular weight of 26.40 kDa.					
32535321	5	59	theme	of → 2	770:775	arg1	-α-l-Rhap-					868:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-	770:877	of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → 	770:882	The backbone of HSP-3 was made up of → 2)-β-d-Manp-(1 → 2,6)-β-d-Manp-(1 → 6)-β-d-Galp-(1 → 3,6)-β-d-Galp-(1 → 4)-α-l-Rhap-(1 → 3,4)-α-l-Rhap-(1 → .					
32535321	1	60	from	polysaccharides	335:349	arg1	HS					354:355	HS	354:355	HS	354:355	Huangshui (HS), the by-product of Chinese Baijiu, has attracted considerable attention due to its nutrient and microbial composition; however, none of the studies has explored the polysaccharides in HS yet.					
32535321	6	61	theme	HSP-3	1163:1167	arg1	properties					1149:1158	significant immunomodulatory properties	1120:1158	significant immunomodulatory properties of HSP-3	1120:1167	Moreover, stimulation of the production of ROS, NO, TNF-α and IL-6, upregulation of the mRNA and protein expression levels of TNF-α and IL-6 in THP-1 cells, and enhanced the pinocytic and phagocytic capacities of THP-1 cells exhibited significant immunomodulatory properties of HSP-3.					
32535321	1	62	theme	considerable	219:230	arg1	attention					232:240	considerable attention	219:240	considerable attention	219:240	Huangshui (HS), the by-product of Chinese Baijiu, has attracted considerable attention due to its nutrient and microbial composition; however, none of the studies has explored the polysaccharides in HS yet.					
33171181	2	0	theme	optimized	480:488	arg1	medium					490:495	the optimized medium	476:495	the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride	476:605	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	5	1	theme	moderate	1139:1146	arg1	efficacy					1162:1169	moderate anticoagulant efficacy	1139:1169	moderate anticoagulant efficacy	1139:1169	Further, the isolated EPS exhibited potent antioxidant activity and moderate anticoagulant efficacy whereas there was no hemolytic and lymphocytes toxicity.					
33171181	0	2	theme	MKU	98:100	arg1	SERB2					102:106	the sponge-associated Bacillus subtilis MKU SERB2	58:106	the sponge-associated Bacillus subtilis MKU SERB2	58:106	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	3	3	theme	EPS	663:665	arg1	spectrum					642:649	infrared (FTIR) spectrum	626:649	infrared (FTIR) spectrum of purified EPS	626:665	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	1	4	theme	sponge-associated	167:183	arg1	SERB2					244:248	Bacillus subtilis MKU SERB2	222:248	Bacillus subtilis MKU SERB2	222:248	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	1	4	theme	sponge-associated	167:183	arg1	bacterium					211:219	a potential endosymbiotic bacterium	185:219	a potential endosymbiotic bacterium	185:219	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	3	5	theme	resonance	828:836	arg1	analysis					844:851	13C nuclear magnetic resonance (NMR) analysis	807:851	13C nuclear magnetic resonance (NMR) analysis	807:851	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	4	6	theme	semi-crystalline	888:903	arg1	nature					905:910	the fibrous, porous and semi-crystalline nature	864:910	the fibrous, porous and semi-crystalline nature of EPS	864:917	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	6	7	theme	food	1350:1353	arg1	industries					1374:1383	food and pharmaceutical industries	1350:1383	food and pharmaceutical industries in the future	1350:1397	Overall, our result suggests that the functional and biological properties of the EPS imply the potential applications in food and pharmaceutical industries in the future.					
33171181	5	8	theme	isolated	1084:1091	arg1	EPS					1093:1095	the isolated EPS	1080:1095	the isolated EPS	1080:1095	Further, the isolated EPS exhibited potent antioxidant activity and moderate anticoagulant efficacy whereas there was no hemolytic and lymphocytes toxicity.					
33171181	2	9	theme	sucrose	520:526	arg1	2.5 g/L					579:585	2.5 g/L	579:585	2.5 g/L of calcium chloride	579:605	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	9	theme	sucrose	520:526	arg1	3.0 g/L					555:561	3.0 g/L	555:561	3.0 g/L of peptone	555:572	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	9	theme	sucrose	520:526	arg1	3.5 g/L					529:535	3.5 g/L	529:535	3.5 g/L of yeast extract	529:552	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	9	theme	sucrose	520:526	arg1	11.5 g/L					508:515	11.5 g/L	508:515	11.5 g/L of sucrose	508:526	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	3	10	theme	carboxyl	698:705	arg1	presence					686:693	the presence	682:693	the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition	682:781	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	4	11	theme	O	1041:1041	arg1	presence					1022:1029	the presence	1018:1029	the presence of C, Na, O, N, S, and Cl	1018:1055	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	0	12	theme	biological	125:134	arg1	properties					136:145	its in-vitro biological properties	112:145	its in-vitro biological properties	112:145	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	6	13	theme	pharmaceutical	1359:1372	arg1	industries					1374:1383	food and pharmaceutical industries	1350:1383	food and pharmaceutical industries in the future	1350:1397	Overall, our result suggests that the functional and biological properties of the EPS imply the potential applications in food and pharmaceutical industries in the future.					
33171181	6	14	theme	EPS	1310:1312	arg1	properties					1292:1301	the functional and biological properties	1262:1301	the functional and biological properties of the EPS	1262:1312	Overall, our result suggests that the functional and biological properties of the EPS imply the potential applications in food and pharmaceutical industries in the future.					
33171181	0	15	theme	in-vitro	116:123	arg1	properties					136:145	its in-vitro biological properties	112:145	its in-vitro biological properties	112:145	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	4	16	theme	diffraction	957:967	arg1	analysis					975:982	SEM and X-ray powder diffraction (XRD) analysis	936:982	SEM and X-ray powder diffraction (XRD) analysis	936:982	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	4	17	theme	N	1044:1044	arg1	presence					1022:1029	the presence	1018:1029	the presence of C, Na, O, N, S, and Cl	1018:1055	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	1	18	theme	potential	187:195	arg1	SERB2					244:248	Bacillus subtilis MKU SERB2	222:248	Bacillus subtilis MKU SERB2	222:248	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	1	18	theme	potential	187:195	arg1	bacterium					211:219	a potential endosymbiotic bacterium	185:219	a potential endosymbiotic bacterium	185:219	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	5	19	theme	anticoagulant	1148:1160	arg1	efficacy					1162:1169	moderate anticoagulant efficacy	1139:1169	moderate anticoagulant efficacy	1139:1169	Further, the isolated EPS exhibited potent antioxidant activity and moderate anticoagulant efficacy whereas there was no hemolytic and lymphocytes toxicity.					
33171181	6	20	theme	functional	1266:1275	arg1	properties					1292:1301	the functional and biological properties	1262:1301	the functional and biological properties of the EPS	1262:1312	Overall, our result suggests that the functional and biological properties of the EPS imply the potential applications in food and pharmaceutical industries in the future.					
33171181	2	21	theme	chloride	598:605	arg1	2.5 g/L					579:585	2.5 g/L	579:585	2.5 g/L of calcium chloride	579:605	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	21	theme	chloride	598:605	arg1	3.0 g/L					555:561	3.0 g/L	555:561	3.0 g/L of peptone	555:572	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	21	theme	chloride	598:605	arg1	3.5 g/L					529:535	3.5 g/L	529:535	3.5 g/L of yeast extract	529:552	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	21	theme	chloride	598:605	arg1	11.5 g/L					508:515	11.5 g/L	508:515	11.5 g/L of sucrose	508:526	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	1	22	theme	endosymbiotic	197:209	arg1	SERB2					244:248	Bacillus subtilis MKU SERB2	222:248	Bacillus subtilis MKU SERB2	222:248	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	1	22	theme	endosymbiotic	197:209	arg1	bacterium					211:219	a potential endosymbiotic bacterium	185:219	a potential endosymbiotic bacterium	185:219	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	2	23	theme	EPS	443:445	arg1	yield					434:438	the highest yield	422:438	the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride	422:605	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	1	24	theme	exopolysaccharide	297:313	arg1	production					283:292	the production	279:292	the production of exopolysaccharide (EPS)	279:319	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	2	25	theme	calcium	590:596	arg1	chloride					598:605	calcium chloride	590:605	calcium chloride	590:605	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	4	26	theme	powder	950:955	arg1	diffraction					957:967	X-ray powder diffraction	944:967	X-ray powder diffraction	944:967	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	4	27	theme	C	1034:1034	arg1	presence					1022:1029	the presence	1018:1029	the presence of C, Na, O, N, S, and Cl	1018:1055	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	2	28	theme	rotatable	388:396	arg1	CCRD					406:409	CCRD	406:409	CCRD	406:409	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	28	theme	rotatable	388:396	arg1	design					398:403	The central composite rotatable design	366:403	The central composite rotatable design (CCRD)	366:410	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	6	29	theme	potential	1324:1332	arg1	applications					1334:1345	the potential applications	1320:1345	the potential applications	1320:1345	Overall, our result suggests that the functional and biological properties of the EPS imply the potential applications in food and pharmaceutical industries in the future.					
33171181	3	30	theme	amide	722:726	arg1	presence					686:693	the presence	682:693	the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition	682:781	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	2	31	theme	extract	546:552	arg1	2.5 g/L					579:585	2.5 g/L	579:585	2.5 g/L of calcium chloride	579:605	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	31	theme	extract	546:552	arg1	3.0 g/L					555:561	3.0 g/L	555:561	3.0 g/L of peptone	555:572	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	31	theme	extract	546:552	arg1	3.5 g/L					529:535	3.5 g/L	529:535	3.5 g/L of yeast extract	529:552	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	31	theme	extract	546:552	arg1	11.5 g/L					508:515	11.5 g/L	508:515	11.5 g/L of sucrose	508:526	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	4	32	dep	inferred	996:1003	arg1	demonstrated					1005:1016	demonstrated	1005:1016	inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively	996:1068	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	2	33	theme	highest	426:432	arg1	yield					434:438	the highest yield	422:438	the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride	422:605	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	3	34	theme	hydroxyl	708:715	arg1	presence					686:693	the presence	682:693	the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition	682:781	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	2	35	theme	yeast	540:544	arg1	extract					546:552	yeast extract	540:552	yeast extract	540:552	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	0	36	from	SERB2	102:106	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	0	36	from	SERB2	102:106	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	0	37	dep	Bacillus	80:87	arg1	subtilis					89:96	subtilis	89:96	subtilis	89:96	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	4	38	theme	Na	1037:1038	arg1	presence					1022:1029	the presence	1018:1029	the presence of C, Na, O, N, S, and Cl	1018:1055	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	1	39	theme	Bacillus	222:229	arg1	SERB2					244:248	Bacillus subtilis MKU SERB2	222:248	Bacillus subtilis MKU SERB2	222:248	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	1	39	theme	Bacillus	222:229	arg1	bacterium					211:219	a potential endosymbiotic bacterium	185:219	a potential endosymbiotic bacterium	185:219	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	4	40	theme	porous	877:882	arg1	nature					905:910	the fibrous, porous and semi-crystalline nature	864:910	the fibrous, porous and semi-crystalline nature of EPS	864:917	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	3	41	theme	FTIR	636:639	arg1	spectrum					642:649	infrared (FTIR) spectrum	626:649	infrared (FTIR) spectrum of purified EPS	626:665	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	0	42	theme	exopolysaccharide	35:51	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	0	42	theme	exopolysaccharide	35:51	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	6	43	theme	biological	1281:1290	arg1	properties					1292:1301	the functional and biological properties	1262:1301	the functional and biological properties of the EPS	1262:1312	Overall, our result suggests that the functional and biological properties of the EPS imply the potential applications in food and pharmaceutical industries in the future.					
33171181	4	44	theme	SEM	936:938	arg1	analysis					975:982	SEM and X-ray powder diffraction (XRD) analysis	936:982	SEM and X-ray powder diffraction (XRD) analysis	936:982	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	1	45	theme	MKU	240:242	arg1	SERB2					244:248	Bacillus subtilis MKU SERB2	222:248	Bacillus subtilis MKU SERB2	222:248	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	1	45	theme	MKU	240:242	arg1	bacterium					211:219	a potential endosymbiotic bacterium	185:219	a potential endosymbiotic bacterium	185:219	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	0	46	theme	sponge-associated	62:78	arg1	SERB2					102:106	the sponge-associated Bacillus subtilis MKU SERB2	58:106	the sponge-associated Bacillus subtilis MKU SERB2	58:106	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	2	47	theme	peptone	566:572	arg1	2.5 g/L					579:585	2.5 g/L	579:585	2.5 g/L of calcium chloride	579:605	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	47	theme	peptone	566:572	arg1	3.0 g/L					555:561	3.0 g/L	555:561	3.0 g/L of peptone	555:572	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	47	theme	peptone	566:572	arg1	3.5 g/L					529:535	3.5 g/L	529:535	3.5 g/L of yeast extract	529:552	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	47	theme	peptone	566:572	arg1	11.5 g/L					508:515	11.5 g/L	508:515	11.5 g/L of sucrose	508:526	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	3	48	theme	infrared	626:633	arg1	spectrum					642:649	infrared (FTIR) spectrum	626:649	infrared (FTIR) spectrum of purified EPS	626:665	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	4	49	theme	Cl	1054:1055	arg1	presence					1022:1029	the presence	1018:1029	the presence of C, Na, O, N, S, and Cl	1018:1055	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	4	50	theme	X-ray	944:948	arg1	diffraction					957:967	X-ray powder diffraction	944:967	X-ray powder diffraction	944:967	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	1	51	theme	response	330:337	arg1	RSM					360:362	RSM	360:362	RSM	360:362	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	1	51	theme	response	330:337	arg1	methodology					347:357	response surface methodology	330:357	response surface methodology (RSM)	330:363	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	4	52	theme	fibrous	868:874	arg1	nature					905:910	the fibrous, porous and semi-crystalline nature	864:910	the fibrous, porous and semi-crystalline nature of EPS	864:917	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	4	53	theme	EPS	915:917	arg1	nature					905:910	the fibrous, porous and semi-crystalline nature	864:910	the fibrous, porous and semi-crystalline nature of EPS	864:917	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	3	54	theme	nuclear	811:817	arg1	NMR					839:841	NMR	839:841	NMR	839:841	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	3	54	theme	nuclear	811:817	arg1	resonance					828:836	13C nuclear magnetic resonance	807:836	13C nuclear magnetic resonance (NMR) analysis	807:851	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	1	55	theme	surface	339:345	arg1	RSM					360:362	RSM	360:362	RSM	360:362	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	1	55	theme	surface	339:345	arg1	methodology					347:357	response surface methodology	330:357	response surface methodology (RSM)	330:363	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	3	56	theme	purified	654:661	arg1	EPS					663:665	purified EPS	654:665	purified EPS	654:665	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	2	57	contain	containing	497:506	arg1	medium					490:495	the optimized medium	476:495	the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride	476:605	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	57	contain	containing	497:506	arg2	3.5 g/L					529:535	3.5 g/L	529:535	3.5 g/L of yeast extract	529:552	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	57	contain	containing	497:506	arg2	3.0 g/L					555:561	3.0 g/L	555:561	3.0 g/L of peptone	555:572	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	57	contain	containing	497:506	arg2	11.5 g/L					508:515	11.5 g/L	508:515	11.5 g/L of sucrose	508:526	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	57	contain	containing	497:506	arg2	2.5 g/L					579:585	2.5 g/L	579:585	2.5 g/L of calcium chloride	579:605	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	3	58	theme	magnetic	819:826	arg1	NMR					839:841	NMR	839:841	NMR	839:841	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	3	58	theme	magnetic	819:826	arg1	resonance					828:836	13C nuclear magnetic resonance	807:836	13C nuclear magnetic resonance (NMR) analysis	807:851	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	3	59	theme	structural	760:769	arg1	composition					771:781	their structural composition	754:781	their structural composition	754:781	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	3	60	theme	functional	731:740	arg1	groups					742:747	functional groups	731:747	functional groups	731:747	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	4	61	theme	S	1047:1047	arg1	presence					1022:1029	the presence	1018:1029	the presence of C, Na, O, N, S, and Cl	1018:1055	Moreover, the fibrous, porous and semi-crystalline nature of EPS was confirmed by SEM and X-ray powder diffraction (XRD) analysis and the EDX inferred demonstrated the presence of C, Na, O, N, S, and Cl respectively.					
33171181	5	62	theme	potent	1107:1112	arg1	activity					1126:1133	potent antioxidant activity	1107:1133	potent antioxidant activity	1107:1133	Further, the isolated EPS exhibited potent antioxidant activity and moderate anticoagulant efficacy whereas there was no hemolytic and lymphocytes toxicity.					
33171181	3	63	dep	Fourier	608:614	arg1	transform					616:624	transform	616:624	transform infrared (FTIR) spectrum of purified EPS	616:665	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	6	64	from	industries	1374:1383	arg1	future					1392:1397	future	1392:1397	future	1392:1397	Overall, our result suggests that the functional and biological properties of the EPS imply the potential applications in food and pharmaceutical industries in the future.					
33171181	2	65	theme	composite	378:386	arg1	CCRD					406:409	CCRD	406:409	CCRD	406:409	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	65	theme	composite	378:386	arg1	design					398:403	The central composite rotatable design	366:403	The central composite rotatable design (CCRD)	366:410	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	3	66	theme	13C	807:809	arg1	NMR					839:841	NMR	839:841	NMR	839:841	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	3	66	theme	13C	807:809	arg1	resonance					828:836	13C nuclear magnetic resonance	807:836	13C nuclear magnetic resonance (NMR) analysis	807:851	Fourier transform infrared (FTIR) spectrum of purified EPS indicated that the presence of carboxyl, hydroxyl, and amide as functional groups, and their structural composition was confirmed by 1H and 13C nuclear magnetic resonance (NMR) analysis.					
33171181	5	67	theme	antioxidant	1114:1124	arg1	activity					1126:1133	potent antioxidant activity	1107:1133	potent antioxidant activity	1107:1133	Further, the isolated EPS exhibited potent antioxidant activity and moderate anticoagulant efficacy whereas there was no hemolytic and lymphocytes toxicity.					
33171181	0	68	theme	Bacillus	80:87	arg1	SERB2					102:106	the sponge-associated Bacillus subtilis MKU SERB2	58:106	the sponge-associated Bacillus subtilis MKU SERB2	58:106	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	1	69	dep	Bacillus	222:229	arg1	subtilis					231:238	subtilis	231:238	subtilis	231:238	In this study, the sponge-associated a potential endosymbiotic bacterium, Bacillus subtilis MKU SERB2 was identified and optimized the production of exopolysaccharide (EPS) by using response surface methodology (RSM).					
33171181	5	70	dep	hemolytic	1192:1200	arg1	toxicity					1218:1225	toxicity	1218:1225	toxicity	1218:1225	Further, the isolated EPS exhibited potent antioxidant activity and moderate anticoagulant efficacy whereas there was no hemolytic and lymphocytes toxicity.					
33171181	2	71	theme	central	370:376	arg1	CCRD					406:409	CCRD	406:409	CCRD	406:409	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	2	71	theme	central	370:376	arg1	design					398:403	The central composite rotatable design	366:403	The central composite rotatable design (CCRD)	366:410	The central composite rotatable design (CCRD) exhibited the highest yield of EPS (617.81 μg/mL) obtained from the optimized medium containing 11.5 g/L of sucrose, 3.5 g/L of yeast extract, 3.0 g/L of peptone, and 2.5 g/L of calcium chloride.					
33171181	0	72	from	properties	136:145	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33171181	0	72	from	properties	136:145	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of exopolysaccharide from the sponge-associated Bacillus subtilis MKU SERB2 and its in-vitro biological properties.					
33142651	4	0	theme	similar	526:532	arg1	groups					545:550	similar functional groups	526:550	similar functional groups	526:550	While both WSPs had similar functional groups, more fragmented particles were observed on the surface of large particles of WSP-n than WSP-p.					
33142651	2	1	theme	molecular	372:380	arg1	structures					382:391	their molecular structures	366:391	their molecular structures	366:391	This study aimed to improve understanding of these functional differences by comparing their molecular structures.					
33142651	5	2	theme	1,4-linked	823:832	arg1	residues					856:863	1,4-linked α-d-galacturonic acid residues	823:863	1,4-linked α-d-galacturonic acid residues	823:863	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	4	3	theme	large	611:615	arg1	particles					617:625	large particles	611:625	large particles of WSP-n than WSP-p	611:645	While both WSPs had similar functional groups, more fragmented particles were observed on the surface of large particles of WSP-n than WSP-p.					
33142651	1	4	from	pulp	180:183	arg1	polysaccharides					142:156	water-soluble polysaccharides	128:156	water-soluble polysaccharides from fermented carrot pulp (WSP-p)	128:191	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	5	5	theme	rhamnogalacturonan-I-type	776:800	arg1	polysaccharides					802:816	rhamnogalacturonan-I-type polysaccharides	776:816	rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues	776:863	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	6	6	theme	similar	955:961	arg1	patterns					971:978	similar linkage patterns	955:978	similar linkage patterns	955:978	1H and 13C NMR showed that they had similar linkage patterns.					
33142651	4	7	located	observed	584:591	arg2	particles					569:577	more fragmented particles	553:577	more fragmented particles	553:577	While both WSPs had similar functional groups, more fragmented particles were observed on the surface of large particles of WSP-n than WSP-p.					
33142651	4	7	located	observed	584:591	arg1	surface					600:606	the surface	596:606	the surface of large particles of WSP-n than WSP-p	596:645	While both WSPs had similar functional groups, more fragmented particles were observed on the surface of large particles of WSP-n than WSP-p.					
33142651	5	8	link	1,4-linked	823:832	arg1	residues					856:863	1,4-linked α-d-galacturonic acid residues	823:863	1,4-linked α-d-galacturonic acid residues	823:863	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	4	9	theme	particles	617:625	arg1	surface					600:606	the surface	596:606	the surface of large particles of WSP-n than WSP-p	596:645	While both WSPs had similar functional groups, more fragmented particles were observed on the surface of large particles of WSP-n than WSP-p.					
33142651	5	10	theme	Monosaccharide	648:661	arg1	composition					663:673	Monosaccharide composition	648:673	Monosaccharide composition	648:673	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	6	11	contain	had	951:953	arg2	patterns					971:978	similar linkage patterns	955:978	similar linkage patterns	955:978	1H and 13C NMR showed that they had similar linkage patterns.					
33142651	6	11	contain	had	951:953	arg1	they					946:949	they	946:949	they	946:949	1H and 13C NMR showed that they had similar linkage patterns.					
33142651	0	12	theme	pectic	50:55	arg1	polysaccharides					57:71	pectic polysaccharides	50:71	pectic polysaccharides from carrot pulp	50:88	Probiotic fermentation modifies the structures of pectic polysaccharides from carrot pulp.					
33142651	4	13	theme	WSP-n	630:634	arg1	particles					617:625	large particles	611:625	large particles of WSP-n than WSP-p	611:645	While both WSPs had similar functional groups, more fragmented particles were observed on the surface of large particles of WSP-n than WSP-p.					
33142651	0	14	from	structures	36:45	arg1	pulp					85:88	carrot pulp	78:88	carrot pulp	78:88	Probiotic fermentation modifies the structures of pectic polysaccharides from carrot pulp.					
33142651	5	15	theme	α-d-galacturonic	834:849	arg1	residues					856:863	1,4-linked α-d-galacturonic acid residues	823:863	1,4-linked α-d-galacturonic acid residues	823:863	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	1	16	theme	Previous	91:98	arg1	studies					100:106	Previous studies	91:106	Previous studies	91:106	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	0	17	theme	Probiotic	0:8	arg1	fermentation					10:21	Probiotic fermentation	0:21	Probiotic fermentation	0:21	Probiotic fermentation modifies the structures of pectic polysaccharides from carrot pulp.					
33142651	5	18	contain	containing	765:774	arg1	polysaccharides					748:762	pectic polysaccharides	741:762	pectic polysaccharides	741:762	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	5	18	contain	containing	765:774	arg2	regions					886:892	homogalacturonan regions	869:892	homogalacturonan regions with 1,4-GalpA linkages	869:916	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	5	18	contain	containing	765:774	arg2	polysaccharides					802:816	rhamnogalacturonan-I-type polysaccharides	776:816	rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues	776:863	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	5	19	theme	methylation	679:689	arg1	analysis					691:698	methylation analysis	679:698	methylation analysis	679:698	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	1	20	contain	have	193:196	arg1	polysaccharides					142:156	water-soluble polysaccharides	128:156	water-soluble polysaccharides from fermented carrot pulp (WSP-p)	128:191	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	1	20	contain	have	193:196	arg2	effects					221:227	stronger anti-diabetic effects	198:227	stronger anti-diabetic effects	198:227	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	3	21	theme	Weight-average	394:407	arg1	weights					419:425	Weight-average molecular weights	394:425	Weight-average molecular weights of WSP-p fractions	394:444	Weight-average molecular weights of WSP-p fractions were lower than those of the corresponding WSP-n fractions.					
33142651	5	22	theme	pectic	741:746	arg1	polysaccharides					748:762	pectic polysaccharides	741:762	pectic polysaccharides	741:762	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	7	23	theme	WSP	1037:1039	arg1	fermentation					1021:1032	probiotic fermentation	1011:1032	probiotic fermentation of WSP	1011:1039	These findings suggested that probiotic fermentation of WSP mainly cleaved the linkages between repeating units, and resulted in less polydisperse molecular size distributions.					
33142651	1	24	theme	anti-diabetic	207:219	arg1	effects					221:227	stronger anti-diabetic effects	198:227	stronger anti-diabetic effects	198:227	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	3	25	theme	molecular	409:417	arg1	weights					419:425	Weight-average molecular weights	394:425	Weight-average molecular weights of WSP-p fractions	394:444	Weight-average molecular weights of WSP-p fractions were lower than those of the corresponding WSP-n fractions.					
33142651	6	26	theme	linkage	963:969	arg1	patterns					971:978	similar linkage patterns	955:978	similar linkage patterns	955:978	1H and 13C NMR showed that they had similar linkage patterns.					
33142651	5	27	theme	homogalacturonan	869:884	arg1	regions					886:892	homogalacturonan regions	869:892	homogalacturonan regions with 1,4-GalpA linkages	869:916	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	4	28	contain	had	522:524	arg1	WSPs					517:520	both WSPs	512:520	both WSPs	512:520	While both WSPs had similar functional groups, more fragmented particles were observed on the surface of large particles of WSP-n than WSP-p.					
33142651	4	28	contain	had	522:524	arg2	groups					545:550	similar functional groups	526:550	similar functional groups	526:550	While both WSPs had similar functional groups, more fragmented particles were observed on the surface of large particles of WSP-n than WSP-p.					
33142651	0	29	from	pulp	85:88	arg1	structures					36:45	the structures	32:45	the structures of pectic polysaccharides from carrot pulp	32:88	Probiotic fermentation modifies the structures of pectic polysaccharides from carrot pulp.					
33142651	0	29	from	pulp	85:88	arg1	polysaccharides					57:71	pectic polysaccharides	50:71	pectic polysaccharides from carrot pulp	50:88	Probiotic fermentation modifies the structures of pectic polysaccharides from carrot pulp.					
33142651	1	30	theme	stronger	198:205	arg1	effects					221:227	stronger anti-diabetic effects	198:227	stronger anti-diabetic effects	198:227	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	3	31	theme	corresponding	475:487	arg1	fractions					495:503	the corresponding WSP-n fractions	471:503	the corresponding WSP-n fractions	471:503	Weight-average molecular weights of WSP-p fractions were lower than those of the corresponding WSP-n fractions.					
33142651	4	32	theme	fragmented	558:567	arg1	particles					569:577	more fragmented particles	553:577	more fragmented particles	553:577	While both WSPs had similar functional groups, more fragmented particles were observed on the surface of large particles of WSP-n than WSP-p.					
33142651	7	33	theme	polydisperse	1115:1126	arg1	distributions					1143:1155	less polydisperse molecular size distributions	1110:1155	less polydisperse molecular size distributions	1110:1155	These findings suggested that probiotic fermentation of WSP mainly cleaved the linkages between repeating units, and resulted in less polydisperse molecular size distributions.					
33142651	3	34	theme	WSP-n	489:493	arg1	fractions					495:503	the corresponding WSP-n fractions	471:503	the corresponding WSP-n fractions	471:503	Weight-average molecular weights of WSP-p fractions were lower than those of the corresponding WSP-n fractions.					
33142651	3	35	theme	WSP-p	430:434	arg1	fractions					436:444	WSP-p fractions	430:444	WSP-p fractions	430:444	Weight-average molecular weights of WSP-p fractions were lower than those of the corresponding WSP-n fractions.					
33142651	5	36	theme	1,4-GalpA	899:907	arg1	linkages					909:916	1,4-GalpA linkages	899:916	1,4-GalpA linkages	899:916	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	5	37	theme	acid	851:854	arg1	residues					856:863	1,4-linked α-d-galacturonic acid residues	823:863	1,4-linked α-d-galacturonic acid residues	823:863	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	7	38	theme	molecular	1128:1136	arg1	distributions					1143:1155	less polydisperse molecular size distributions	1110:1155	less polydisperse molecular size distributions	1110:1155	These findings suggested that probiotic fermentation of WSP mainly cleaved the linkages between repeating units, and resulted in less polydisperse molecular size distributions.					
33142651	3	39	theme	fractions	436:444	arg1	weights					419:425	Weight-average molecular weights	394:425	Weight-average molecular weights of WSP-p fractions	394:444	Weight-average molecular weights of WSP-p fractions were lower than those of the corresponding WSP-n fractions.					
33142651	5	40	with	polysaccharides	802:816	arg1	residues					856:863	1,4-linked α-d-galacturonic acid residues	823:863	1,4-linked α-d-galacturonic acid residues	823:863	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	5	40	with	polysaccharides	802:816	arg1	linkages					909:916	1,4-GalpA linkages	899:916	1,4-GalpA linkages	899:916	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	1	41	theme	water-soluble	128:140	arg1	polysaccharides					142:156	water-soluble polysaccharides	128:156	water-soluble polysaccharides from fermented carrot pulp (WSP-p)	128:191	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	7	42	theme	probiotic	1011:1019	arg1	fermentation					1021:1032	probiotic fermentation	1011:1032	probiotic fermentation of WSP	1011:1039	These findings suggested that probiotic fermentation of WSP mainly cleaved the linkages between repeating units, and resulted in less polydisperse molecular size distributions.					
33142651	7	43	theme	size	1138:1141	arg1	distributions					1143:1155	less polydisperse molecular size distributions	1110:1155	less polydisperse molecular size distributions	1110:1155	These findings suggested that probiotic fermentation of WSP mainly cleaved the linkages between repeating units, and resulted in less polydisperse molecular size distributions.					
33142651	7	44	theme	repeating	1077:1085	arg1	units					1087:1091	repeating units	1077:1091	repeating units	1077:1091	These findings suggested that probiotic fermentation of WSP mainly cleaved the linkages between repeating units, and resulted in less polydisperse molecular size distributions.					
33142651	2	45	theme	differences	341:351	arg1	understanding					307:319	understanding	307:319	understanding of these functional differences	307:351	This study aimed to improve understanding of these functional differences by comparing their molecular structures.					
33142651	5	46	with	regions	886:892	arg1	residues					856:863	1,4-linked α-d-galacturonic acid residues	823:863	1,4-linked α-d-galacturonic acid residues	823:863	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	5	46	with	regions	886:892	arg1	linkages					909:916	1,4-GalpA linkages	899:916	1,4-GalpA linkages	899:916	Monosaccharide composition and methylation analysis confirmed that both WSP-p and WSP-n were pectic polysaccharides, containing rhamnogalacturonan-I-type polysaccharides with 1,4-linked α-d-galacturonic acid residues and homogalacturonan regions with 1,4-GalpA linkages.					
33142651	2	47	theme	functional	330:339	arg1	differences					341:351	these functional differences	324:351	these functional differences	324:351	This study aimed to improve understanding of these functional differences by comparing their molecular structures.					
33142651	1	48	theme	un-fermented	245:256	arg1	WSP-n					271:275	WSP-n	271:275	WSP-n	271:275	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	1	48	theme	un-fermented	245:256	arg1	pulp					265:268	un-fermented carrot pulp	245:268	un-fermented carrot pulp (WSP-n)	245:276	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	0	49	theme	carrot	78:83	arg1	pulp					85:88	carrot pulp	78:88	carrot pulp	78:88	Probiotic fermentation modifies the structures of pectic polysaccharides from carrot pulp.					
33142651	6	50	theme	13C	926:928	arg1	NMR					930:932	13C NMR	926:932	13C NMR	926:932	1H and 13C NMR showed that they had similar linkage patterns.					
33142651	1	51	theme	fermented	163:171	arg1	WSP-p					186:190	WSP-p	186:190	WSP-p	186:190	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	1	51	theme	fermented	163:171	arg1	pulp					180:183	fermented carrot pulp	163:183	fermented carrot pulp (WSP-p)	163:191	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	1	52	theme	carrot	258:263	arg1	WSP-n					271:275	WSP-n	271:275	WSP-n	271:275	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	1	52	theme	carrot	258:263	arg1	pulp					265:268	un-fermented carrot pulp	245:268	un-fermented carrot pulp (WSP-n)	245:276	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	0	53	theme	polysaccharides	57:71	arg1	structures					36:45	the structures	32:45	the structures of pectic polysaccharides from carrot pulp	32:88	Probiotic fermentation modifies the structures of pectic polysaccharides from carrot pulp.					
33142651	4	54	theme	functional	534:543	arg1	groups					545:550	similar functional groups	526:550	similar functional groups	526:550	While both WSPs had similar functional groups, more fragmented particles were observed on the surface of large particles of WSP-n than WSP-p.					
33142651	1	55	theme	carrot	173:178	arg1	WSP-p					186:190	WSP-p	186:190	WSP-p	186:190	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
33142651	1	55	theme	carrot	173:178	arg1	pulp					180:183	fermented carrot pulp	163:183	fermented carrot pulp (WSP-p)	163:191	Previous studies have suggested that water-soluble polysaccharides from fermented carrot pulp (WSP-p) have stronger anti-diabetic effects than those from un-fermented carrot pulp (WSP-n).					
32304790	5	0	theme	Differential	1176:1187	arg1	scattering					1128:1137	multi-laser light scattering	1110:1137	multi-laser light scattering (SEC-MALLS)	1110:1149	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	0	theme	Differential	1176:1187	arg1	DSC					1211:1213	DSC	1211:1213	DSC	1211:1213	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	0	theme	Differential	1176:1187	arg1	Calorimetry					1198:1208	Differential Scanning Calorimetry	1176:1208	Differential Scanning Calorimetry (DSC)	1176:1214	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	3	1	theme	different	544:552	arg1	types					554:558	same or different types	536:558	same or different types	536:558	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	6	2	theme	microbial	1332:1340	arg1	composition					1388:1398	composition	1388:1398	composition	1388:1398	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	2	theme	microbial	1332:1340	arg1	exopolysaccharides					1342:1359	microbial exopolysaccharides	1332:1359	microbial exopolysaccharides	1332:1359	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	3	theme	possible	1436:1443	arg1	applications					1456:1467	possible commercial applications	1436:1467	possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry	1436:1542	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	5	4	theme	Scanning	1189:1196	arg1	scattering					1128:1137	multi-laser light scattering	1110:1137	multi-laser light scattering (SEC-MALLS)	1110:1149	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	4	theme	Scanning	1189:1196	arg1	DSC					1211:1213	DSC	1211:1213	DSC	1211:1213	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	4	theme	Scanning	1189:1196	arg1	Calorimetry					1198:1208	Differential Scanning Calorimetry	1176:1208	Differential Scanning Calorimetry (DSC)	1176:1214	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	6	5	theme	current	1303:1309	arg1	article					1311:1317	the current article	1299:1317	the current article	1299:1317	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	3	6	theme	types	554:558	arg1	units					527:531	repeated sugar units	512:531	repeated sugar units of same or different types	512:558	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	3	7	theme	protective	697:706	arg1	layer					708:712	a protective layer	695:712	a protective layer around the microbial colonies collectively known as biofilm	695:772	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	5	8	theme	Exchange	1007:1014	arg1	Chromatography					1016:1029	High Performance Anion Exchange Chromatography	984:1029	High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection	984:1064	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	8	theme	Exchange	1007:1014	arg1	Chromatography					1082:1095	Size Exclusion Chromatography	1067:1095	Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC)	1067:1214	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	9	theme	Anion	1001:1005	arg1	Chromatography					1016:1029	High Performance Anion Exchange Chromatography	984:1029	High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection	984:1064	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	9	theme	Anion	1001:1005	arg1	Chromatography					1082:1095	Size Exclusion Chromatography	1067:1095	Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC)	1067:1214	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	4	10	theme	Specific	775:782	arg1	functions					784:792	Specific functions	775:792	Specific functions of exopolysaccharides	775:814	Specific functions of exopolysaccharides depend on structural composition and habitat of a host microorganism.					
32304790	2	11	theme	microbial	295:303	arg1	exopolysaccharides					305:322	microbial exopolysaccharides	295:322	microbial exopolysaccharides	295:322	Among all the life forms microbial exopolysaccharides are produced in shorter time duration as they responsible for the microbial cell adhesion and protection during unfavorable growth conditions.					
32304790	2	11	theme	microbial	295:303	arg1	they					365:368	they	365:368	they	365:368	Among all the life forms microbial exopolysaccharides are produced in shorter time duration as they responsible for the microbial cell adhesion and protection during unfavorable growth conditions.					
32304790	5	12	theme	Thermal	1255:1261	arg1	etc					1291:1293	etc	1291:1293	etc	1291:1293	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	12	theme	Thermal	1255:1261	arg1	Analysis					1275:1282	Thermal Gravimetric Analysis	1255:1282	Thermal Gravimetric Analysis (TGA)	1255:1288	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	12	theme	Thermal	1255:1261	arg1	TGA					1285:1287	TGA	1285:1287	TGA	1285:1287	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	3	13	theme	same	536:539	arg1	types					554:558	same or different types	536:558	same or different types	536:558	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	4	14	theme	microorganism	871:883	arg1	habitat					853:859	habitat	853:859	habitat	853:859	Specific functions of exopolysaccharides depend on structural composition and habitat of a host microorganism.					
32304790	4	14	theme	microorganism	871:883	arg1	composition					837:847	composition	837:847	composition	837:847	Specific functions of exopolysaccharides depend on structural composition and habitat of a host microorganism.					
32304790	2	15	theme	growth	448:453	arg1	conditions					455:464	unfavorable growth conditions	436:464	unfavorable growth conditions	436:464	Among all the life forms microbial exopolysaccharides are produced in shorter time duration as they responsible for the microbial cell adhesion and protection during unfavorable growth conditions.					
32304790	4	16	theme	exopolysaccharides	797:814	arg1	functions					784:792	Specific functions	775:792	Specific functions of exopolysaccharides	775:814	Specific functions of exopolysaccharides depend on structural composition and habitat of a host microorganism.					
32304790	4	17	theme	host	866:869	arg1	microorganism					871:883	a host microorganism	864:883	a host microorganism	864:883	Specific functions of exopolysaccharides depend on structural composition and habitat of a host microorganism.					
32304790	2	18	theme	life	284:287	arg1	forms					289:293	all the life forms	276:293	all the life forms	276:293	Among all the life forms microbial exopolysaccharides are produced in shorter time duration as they responsible for the microbial cell adhesion and protection during unfavorable growth conditions.					
32304790	5	19	theme	various	896:902	arg1	techniques					904:913	various techniques	896:913	various techniques	896:913	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	3	20	theme	microbial	725:733	arg1	colonies					735:742	the microbial colonies	721:742	the microbial colonies collectively known as biofilm	721:772	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	6	21	theme	commercial	1445:1454	arg1	applications					1456:1467	possible commercial applications	1436:1467	possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry	1436:1542	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	2	22	theme	shorter	340:346	arg1	duration					353:360	shorter time duration	340:360	shorter time duration	340:360	Among all the life forms microbial exopolysaccharides are produced in shorter time duration as they responsible for the microbial cell adhesion and protection during unfavorable growth conditions.					
32304790	0	23	theme	Microbial	0:8	arg1	exopolysaccharides					10:27	Microbial exopolysaccharides	0:27	Microbial exopolysaccharides	0:27	Microbial exopolysaccharides: Synthesis pathways, types and their commercial applications.					
32304790	5	24	theme	High	984:987	arg1	Chromatography					1016:1029	High Performance Anion Exchange Chromatography	984:1029	High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection	984:1064	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	24	theme	High	984:987	arg1	Chromatography					1082:1095	Size Exclusion Chromatography	1067:1095	Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC)	1067:1214	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	2	25	theme	cell	400:403	arg1	adhesion					405:412	microbial cell adhesion	390:412	microbial cell adhesion	390:412	Among all the life forms microbial exopolysaccharides are produced in shorter time duration as they responsible for the microbial cell adhesion and protection during unfavorable growth conditions.					
32304790	0	26	theme	Synthesis	30:38	arg1	pathways					40:47	Synthesis pathways	30:47	Synthesis pathways	30:47	Microbial exopolysaccharides: Synthesis pathways, types and their commercial applications.					
32304790	1	27	theme	essential	111:119	arg1	metabolites					129:139	essential natural metabolites	111:139	essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions	111:267	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	27	theme	essential	111:119	arg1	Polysaccharides					91:105	Polysaccharides	91:105	Polysaccharides	91:105	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	3	28	theme	repeated	512:519	arg1	units					527:531	repeated sugar units	512:531	repeated sugar units of same or different types	512:558	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	3	29	theme	organic	655:661	arg1	compounds					677:685	organic and inorganic compounds	655:685	organic and inorganic compounds	655:685	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	1	30	theme	natural	121:127	arg1	metabolites					129:139	essential natural metabolites	111:139	essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions	111:267	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	30	theme	natural	121:127	arg1	Polysaccharides					91:105	Polysaccharides	91:105	Polysaccharides	91:105	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	31	theme	life	154:157	arg1	animals					189:195	animals	189:195	animals	189:195	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	31	theme	life	154:157	arg1	forms					159:163	all life forms	150:163	all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions	150:267	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	31	theme	life	154:157	arg1	microorganisms					173:186	microorganisms	173:186	microorganisms	173:186	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	31	theme	life	154:157	arg1	plants					201:206	plants	201:206	plants	201:206	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	32	with	plants	201:206	arg1	structures					233:242	various biochemical structures	213:242	various biochemical structures	213:242	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	32	with	plants	201:206	arg1	functions					259:267	biological functions	248:267	biological functions	248:267	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	33	theme	various	213:219	arg1	structures					233:242	various biochemical structures	213:242	various biochemical structures	213:242	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	5	34	theme	Exclusion	1072:1080	arg1	Chromatography					1082:1095	Size Exclusion Chromatography	1067:1095	Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC)	1067:1214	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	34	theme	Exclusion	1072:1080	arg1	Chromatography					1016:1029	High Performance Anion Exchange Chromatography	984:1029	High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection	984:1064	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	3	35	theme	Microbial	467:475	arg1	exopolysaccharides					477:494	Microbial exopolysaccharides	467:494	Microbial exopolysaccharides	467:494	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	2	36	theme	unfavorable	436:446	arg1	conditions					455:464	unfavorable growth conditions	436:464	unfavorable growth conditions	436:464	Among all the life forms microbial exopolysaccharides are produced in shorter time duration as they responsible for the microbial cell adhesion and protection during unfavorable growth conditions.					
32304790	6	37	theme	dairy	1472:1476	arg1	research					1499:1506	research	1499:1506	research	1499:1506	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	37	theme	dairy	1472:1476	arg1	petroleum					1525:1533	petroleum	1525:1533	petroleum	1525:1533	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	37	theme	dairy	1472:1476	arg1	products					1478:1485	dairy products	1472:1485	dairy products	1472:1485	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	37	theme	dairy	1472:1476	arg1	agriculture					1509:1519	agriculture	1509:1519	agriculture	1509:1519	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	37	theme	dairy	1472:1476	arg1	cosmetics					1488:1496	cosmetics	1488:1496	cosmetics	1488:1496	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	38	dep	exopolysaccharides	1342:1359	arg1	properties					1376:1385	physiochemical properties	1361:1385	physiochemical properties	1361:1385	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	39	theme	physiochemical	1361:1374	arg1	properties					1376:1385	physiochemical properties	1361:1385	physiochemical properties	1361:1385	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	40	dep	agriculture	1509:1519	arg1	industry					1535:1542	industry	1535:1542	industry	1535:1542	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	1	41	theme	biochemical	221:231	arg1	structures					233:242	various biochemical structures	213:242	various biochemical structures	213:242	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	42	with	animals	189:195	arg1	structures					233:242	various biochemical structures	213:242	various biochemical structures	213:242	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	42	with	animals	189:195	arg1	functions					259:267	biological functions	248:267	biological functions	248:267	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	5	43	theme	X-Ray	1151:1155	arg1	XRD					1170:1172	XRD	1170:1172	XRD	1170:1172	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	43	theme	X-Ray	1151:1155	arg1	scattering					1128:1137	multi-laser light scattering	1110:1137	multi-laser light scattering (SEC-MALLS)	1110:1149	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	43	theme	X-Ray	1151:1155	arg1	diffraction					1157:1167	X-Ray diffraction	1151:1167	X-Ray diffraction (XRD)	1151:1173	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	3	44	theme	sugar	521:525	arg1	units					527:531	repeated sugar units	512:531	repeated sugar units of same or different types	512:558	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	5	45	with	Chromatography	1016:1029	arg1	Detection					1056:1064	Pulsed Amperometric Detection	1036:1064	Pulsed Amperometric Detection	1036:1064	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	6	46	from	applications	1456:1467	arg1	research					1499:1506	research	1499:1506	research	1499:1506	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	46	from	applications	1456:1467	arg1	petroleum					1525:1533	petroleum	1525:1533	petroleum	1525:1533	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	46	from	applications	1456:1467	arg1	products					1478:1485	dairy products	1472:1485	dairy products	1472:1485	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	46	from	applications	1456:1467	arg1	agriculture					1509:1519	agriculture	1509:1519	agriculture	1509:1519	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	6	46	from	applications	1456:1467	arg1	cosmetics					1488:1496	cosmetics	1488:1496	cosmetics	1488:1496	In the current article, we reviewed microbial exopolysaccharides physiochemical properties, composition, analyzing techniques through which possible commercial applications in dairy products, cosmetics, research, agriculture and petroleum industry can be performed.					
32304790	5	47	dep	Infrared	1235:1242	arg1	FTIR					1245:1248	FTIR	1245:1248	FTIR	1245:1248	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	48	theme	multi-laser	1110:1120	arg1	scattering					1128:1137	multi-laser light scattering	1110:1137	multi-laser light scattering (SEC-MALLS)	1110:1149	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	48	theme	multi-laser	1110:1120	arg1	diffraction					1157:1167	X-Ray diffraction	1151:1167	X-Ray diffraction (XRD)	1151:1173	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	48	theme	multi-laser	1110:1120	arg1	SEC-MALLS					1140:1148	SEC-MALLS	1140:1148	SEC-MALLS	1140:1148	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	48	theme	multi-laser	1110:1120	arg1	Calorimetry					1198:1208	Differential Scanning Calorimetry	1176:1208	Differential Scanning Calorimetry (DSC)	1176:1214	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	3	49	theme	metal	617:621	arg1	ions					623:626	metal ions	617:626	metal ions	617:626	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	3	49	theme	metal	617:621	arg1	proteins					599:606	proteins	599:606	proteins	599:606	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	5	50	theme	Size	1067:1070	arg1	Chromatography					1082:1095	Size Exclusion Chromatography	1067:1095	Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC)	1067:1214	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	50	theme	Size	1067:1070	arg1	Chromatography					1016:1029	High Performance Anion Exchange Chromatography	984:1029	High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection	984:1064	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	1	51	with	microorganisms	173:186	arg1	structures					233:242	various biochemical structures	213:242	various biochemical structures	213:242	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	51	with	microorganisms	173:186	arg1	functions					259:267	biological functions	248:267	biological functions	248:267	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	5	52	theme	light	1122:1126	arg1	scattering					1128:1137	multi-laser light scattering	1110:1137	multi-laser light scattering (SEC-MALLS)	1110:1149	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	52	theme	light	1122:1126	arg1	diffraction					1157:1167	X-Ray diffraction	1151:1167	X-Ray diffraction (XRD)	1151:1173	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	52	theme	light	1122:1126	arg1	SEC-MALLS					1140:1148	SEC-MALLS	1140:1148	SEC-MALLS	1140:1148	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	52	theme	light	1122:1126	arg1	Calorimetry					1198:1208	Differential Scanning Calorimetry	1176:1208	Differential Scanning Calorimetry (DSC)	1176:1214	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	2	53	dep	adhesion	405:412	arg1	the					386:388	the	386:388	the	386:388	Among all the life forms microbial exopolysaccharides are produced in shorter time duration as they responsible for the microbial cell adhesion and protection during unfavorable growth conditions.					
32304790	5	54	theme	exopolysaccharides	957:974	arg1	composition					928:938	the composition	924:938	the composition	924:938	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	54	theme	exopolysaccharides	957:974	arg1	Analysis					1275:1282	Thermal Gravimetric Analysis	1255:1282	Thermal Gravimetric Analysis (TGA)	1255:1288	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	54	theme	exopolysaccharides	957:974	arg1	TGA					1285:1287	TGA	1285:1287	TGA	1285:1287	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	54	theme	exopolysaccharides	957:974	arg1	structure					944:952	structure	944:952	structure	944:952	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	54	theme	exopolysaccharides	957:974	arg1	etc					1291:1293	etc	1291:1293	etc	1291:1293	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	55	theme	Performance	989:999	arg1	Chromatography					1016:1029	High Performance Anion Exchange Chromatography	984:1029	High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection	984:1064	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	55	theme	Performance	989:999	arg1	Chromatography					1082:1095	Size Exclusion Chromatography	1067:1095	Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC)	1067:1214	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	56	theme	Gravimetric	1263:1273	arg1	etc					1291:1293	etc	1291:1293	etc	1291:1293	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	56	theme	Gravimetric	1263:1273	arg1	Analysis					1275:1282	Thermal Gravimetric Analysis	1255:1282	Thermal Gravimetric Analysis (TGA)	1255:1288	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	56	theme	Gravimetric	1263:1273	arg1	TGA					1285:1287	TGA	1285:1287	TGA	1285:1287	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	5	57	theme	Pulsed	1036:1041	arg1	Detection					1056:1064	Pulsed Amperometric Detection	1036:1064	Pulsed Amperometric Detection	1036:1064	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	3	58	theme	inorganic	667:675	arg1	compounds					677:685	organic and inorganic compounds	655:685	organic and inorganic compounds	655:685	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	5	59	dep	exopolysaccharides	957:974	arg1	Transform					1225:1233	Transform	1225:1233	Transform Infrared (FTIR)	1225:1249	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	2	60	theme	time	348:351	arg1	duration					353:360	shorter time duration	340:360	shorter time duration	340:360	Among all the life forms microbial exopolysaccharides are produced in shorter time duration as they responsible for the microbial cell adhesion and protection during unfavorable growth conditions.					
32304790	0	61	theme	commercial	66:75	arg1	applications					77:88	their commercial applications	60:88	their commercial applications	60:88	Microbial exopolysaccharides: Synthesis pathways, types and their commercial applications.					
32304790	3	62	theme	extracellular	629:641	arg1	DNA					643:645	extracellular DNA	629:645	extracellular DNA (eDNA)	629:652	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	3	62	theme	extracellular	629:641	arg1	proteins					599:606	proteins	599:606	proteins	599:606	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	3	62	theme	extracellular	629:641	arg1	eDNA					648:651	eDNA	648:651	eDNA	648:651	Microbial exopolysaccharides are composed of repeated sugar units of same or different types and form a complex by associating with proteins, lipids, metal ions, extracellular DNA (eDNA), organic and inorganic compounds to form a protective layer around the microbial colonies collectively known as biofilm.					
32304790	2	63	theme	microbial	390:398	arg1	adhesion					405:412	microbial cell adhesion	390:412	microbial cell adhesion	390:412	Among all the life forms microbial exopolysaccharides are produced in shorter time duration as they responsible for the microbial cell adhesion and protection during unfavorable growth conditions.					
32304790	1	64	theme	biological	248:257	arg1	functions					259:267	biological functions	248:267	biological functions	248:267	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	5	65	theme	Amperometric	1043:1054	arg1	Detection					1056:1064	Pulsed Amperometric Detection	1036:1064	Pulsed Amperometric Detection	1036:1064	There are various techniques to study the composition and structure of exopolysaccharides such as High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection, Size Exclusion Chromatography coupled with multi-laser light scattering (SEC-MALLS),X-Ray diffraction (XRD), Differential Scanning Calorimetry (DSC), Fourier Transform Infrared (FTIR) and Thermal Gravimetric Analysis (TGA), etc.					
32304790	1	66	located	found	141:145	arg1	animals					189:195	animals	189:195	animals	189:195	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	66	located	found	141:145	arg1	forms					159:163	all life forms	150:163	all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions	150:267	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	66	located	found	141:145	arg2	Polysaccharides					91:105	Polysaccharides	91:105	Polysaccharides	91:105	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	66	located	found	141:145	arg1	plants					201:206	plants	201:206	plants	201:206	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	66	located	found	141:145	arg2	metabolites					129:139	essential natural metabolites	111:139	essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions	111:267	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	1	66	located	found	141:145	arg1	microorganisms					173:186	microorganisms	173:186	microorganisms	173:186	Polysaccharides are essential natural metabolites found in all life forms such as microorganisms, animals and plants with various biochemical structures and biological functions.					
32304790	4	67	theme	structural	826:835	arg1	composition					837:847	composition	837:847	composition	837:847	Specific functions of exopolysaccharides depend on structural composition and habitat of a host microorganism.					
33940104	4	0	theme	original	945:952	arg1	polysaccharides					954:968	original polysaccharides	945:968	original polysaccharides in RAW 264.7 cells	945:987	Besides, polysaccharides after UV/H2O2 treatment for 0.5-2 h had lower toxicity than original polysaccharides in RAW 264.7 cells.					
33940104	1	1	theme	seaweed	159:165	arg1	polysaccharides					167:181	seaweed polysaccharides	159:181	seaweed polysaccharides with UV/H2O2 treatment	159:204	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	3	2	theme	polysaccharides	768:782	arg1	types					750:754	the monosaccharide types	731:754	the monosaccharide types of original polysaccharides	731:782	In addition, UV/H2O2 treatment did not change the monosaccharide types of original polysaccharides, but it could change its monosaccharide composition and surface morphology.					
33940104	0	3	theme	Physicochemical	76:90	arg1	properties					92:101	Physicochemical properties	76:101	Physicochemical properties	76:101	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme: Physicochemical properties and RAW 264.7 cells responses.					
33940104	1	4	theme	polysaccharides	344:358	arg1	responses					331:339	RAW 264.7 cells responses	315:339	RAW 264.7 cells responses	315:339	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	4	theme	polysaccharides	344:358	arg1	properties					300:309	physicochemical properties	284:309	physicochemical properties	284:309	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	5	with	polysaccharides	167:181	arg1	treatment					196:204	UV/H2O2 treatment	188:204	UV/H2O2 treatment	188:204	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	0	6	theme	RAW	107:109	arg1	responses					123:131	RAW 264.7 cells responses	107:131	RAW 264.7 cells responses	107:131	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme: Physicochemical properties and RAW 264.7 cells responses.					
33940104	0	7	from	Influence	0:8	arg1	polysaccharides					34:48	polysaccharides	34:48	polysaccharides from Sargassum fusiforme	34:73	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme: Physicochemical properties and RAW 264.7 cells responses.					
33940104	1	8	from	properties	300:309	arg1	fusiforme					375:383	Sargassum fusiforme	365:383	Sargassum fusiforme (PSF)	365:389	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	8	from	properties	300:309	arg1	PSF					386:388	PSF	386:388	PSF	386:388	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	3	9	theme	monosaccharide	809:822	arg1	composition					824:834	its monosaccharide composition	805:834	its monosaccharide composition	805:834	In addition, UV/H2O2 treatment did not change the monosaccharide types of original polysaccharides, but it could change its monosaccharide composition and surface morphology.					
33940104	5	10	with	PSF	1001:1003	arg1	treatment					1018:1026	UV/H2O2 treatment	1010:1026	UV/H2O2 treatment	1010:1026	Typically, PSF with UV/H2O2 treatment for 2 h (PSF-T2) could effectively inhibit pro-inflammatory molecules production (including NO, IL-1β, IL-6 and TNF-α), and down-regulate related genes expression (including Tlr4, Irak, Il-1β, Il-6, Il-12 and Tnf-α).					
33940104	1	11	theme	Sargassum	365:373	arg1	fusiforme					375:383	Sargassum fusiforme	365:383	Sargassum fusiforme (PSF)	365:389	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	11	theme	Sargassum	365:373	arg1	PSF					386:388	PSF	386:388	PSF	386:388	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	12	from	studies	148:154	arg1	polysaccharides					167:181	seaweed polysaccharides	159:181	seaweed polysaccharides with UV/H2O2 treatment	159:204	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	3	13	theme	original	759:766	arg1	polysaccharides					768:782	original polysaccharides	759:782	original polysaccharides	759:782	In addition, UV/H2O2 treatment did not change the monosaccharide types of original polysaccharides, but it could change its monosaccharide composition and surface morphology.					
33940104	2	14	theme	acid	592:595	arg1	contents					548:555	the contents	544:555	the contents of total sugar, protein and uronic acid	544:595	Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides.					
33940104	5	15	theme	related	1166:1172	arg1	expression					1180:1189	related genes expression	1166:1189	related genes expression (including Tlr4, Irak, Il-1β, Il-6, Il-12 and Tnf-α)	1166:1242	Typically, PSF with UV/H2O2 treatment for 2 h (PSF-T2) could effectively inhibit pro-inflammatory molecules production (including NO, IL-1β, IL-6 and TNF-α), and down-regulate related genes expression (including Tlr4, Irak, Il-1β, Il-6, Il-12 and Tnf-α).					
33940104	0	16	theme	264.7 cells	111:121	arg1	responses					123:131	RAW 264.7 cells responses	107:131	RAW 264.7 cells responses	107:131	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme: Physicochemical properties and RAW 264.7 cells responses.					
33940104	2	17	theme	uronic	585:590	arg1	acid					592:595	uronic acid	585:595	uronic acid	585:595	Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides.					
33940104	3	18	theme	surface	840:846	arg1	morphology					848:857	surface morphology	840:857	surface morphology	840:857	In addition, UV/H2O2 treatment did not change the monosaccharide types of original polysaccharides, but it could change its monosaccharide composition and surface morphology.					
33940104	1	19	theme	UV/H2O2	188:194	arg1	treatment					196:204	UV/H2O2 treatment	188:204	UV/H2O2 treatment	188:204	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	5	20	theme	pro-inflammatory	1071:1086	arg1	molecules					1088:1096	pro-inflammatory molecules	1071:1096	pro-inflammatory molecules production (including NO, IL-1β, IL-6 and TNF-α)	1071:1145	Typically, PSF with UV/H2O2 treatment for 2 h (PSF-T2) could effectively inhibit pro-inflammatory molecules production (including NO, IL-1β, IL-6 and TNF-α), and down-regulate related genes expression (including Tlr4, Irak, Il-1β, Il-6, Il-12 and Tnf-α).					
33940104	0	21	dep	properties	92:101	arg1	Influence					0:8	Influence	0:8	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme	0:73	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme: Physicochemical properties and RAW 264.7 cells responses.					
33940104	6	22	theme	better	1324:1329	arg1	activity					1349:1356	better anti-inflammatory activity	1324:1356	better anti-inflammatory activity	1324:1356	Therefore, UV/H2O2 treatment is a potential way to prepare polysaccharide with better anti-inflammatory activity.					
33940104	2	23	from	sulfate	447:453	arg1	PSF					458:460	PSF	458:460	PSF	458:460	Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides.					
33940104	1	24	theme	UV/H2O2	263:269	arg1	treatment					271:279	UV/H2O2 treatment	263:279	UV/H2O2 treatment	263:279	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	2	25	theme	protein	573:579	arg1	contents					548:555	the contents	544:555	the contents of total sugar, protein and uronic acid	544:595	Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides.					
33940104	2	26	theme	sugar	566:570	arg1	contents					548:555	the contents	544:555	the contents of total sugar, protein and uronic acid	544:595	Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides.					
33940104	0	27	theme	treatment	21:29	arg1	Influence					0:8	Influence	0:8	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme	0:73	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme: Physicochemical properties and RAW 264.7 cells responses.					
33940104	1	28	theme	treatment	271:279	arg1	effects					252:258	the effects	248:258	the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF)	248:389	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	29	from	effects	252:258	arg1	responses					331:339	RAW 264.7 cells responses	315:339	RAW 264.7 cells responses	315:339	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	29	from	effects	252:258	arg1	properties					300:309	physicochemical properties	284:309	physicochemical properties	284:309	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	30	from	fusiforme	375:383	arg1	polysaccharides					344:358	polysaccharides	344:358	polysaccharides from Sargassum fusiforme (PSF)	344:389	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	30	from	fusiforme	375:383	arg1	responses					331:339	RAW 264.7 cells responses	315:339	RAW 264.7 cells responses	315:339	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	30	from	fusiforme	375:383	arg1	properties					300:309	physicochemical properties	284:309	physicochemical properties	284:309	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	2	31	theme	UV/H2O2	467:473	arg1	treatment					475:483	UV/H2O2 treatment	467:483	UV/H2O2 treatment for 2 h	467:491	Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides.					
33940104	1	32	theme	physicochemical	284:298	arg1	properties					300:309	physicochemical properties	284:309	physicochemical properties	284:309	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	4	33	theme	UV/H2O2	891:897	arg1	treatment					899:907	UV/H2O2 treatment	891:907	UV/H2O2 treatment for 0.5-2 h	891:919	Besides, polysaccharides after UV/H2O2 treatment for 0.5-2 h had lower toxicity than original polysaccharides in RAW 264.7 cells.					
33940104	6	34	theme	anti-inflammatory	1331:1347	arg1	activity					1349:1356	better anti-inflammatory activity	1324:1356	better anti-inflammatory activity	1324:1356	Therefore, UV/H2O2 treatment is a potential way to prepare polysaccharide with better anti-inflammatory activity.					
33940104	3	35	theme	UV/H2O2	698:704	arg1	treatment					706:714	UV/H2O2 treatment	698:714	UV/H2O2 treatment	698:714	In addition, UV/H2O2 treatment did not change the monosaccharide types of original polysaccharides, but it could change its monosaccharide composition and surface morphology.					
33940104	6	36	theme	UV/H2O2	1256:1262	arg1	treatment					1264:1272	UV/H2O2 treatment	1256:1272	UV/H2O2 treatment	1256:1272	Therefore, UV/H2O2 treatment is a potential way to prepare polysaccharide with better anti-inflammatory activity.					
33940104	6	36	theme	UV/H2O2	1256:1262	arg1	way					1289:1291	a potential way	1277:1291	a potential way to prepare polysaccharide with better anti-inflammatory activity	1277:1356	Therefore, UV/H2O2 treatment is a potential way to prepare polysaccharide with better anti-inflammatory activity.					
33940104	4	37	from	polysaccharides	954:968	arg1	264.7 cells					977:987	RAW 264.7 cells	973:987	RAW 264.7 cells	973:987	Besides, polysaccharides after UV/H2O2 treatment for 0.5-2 h had lower toxicity than original polysaccharides in RAW 264.7 cells.					
33940104	0	38	theme	Sargassum	55:63	arg1	fusiforme					65:73	Sargassum fusiforme	55:73	Sargassum fusiforme	55:73	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme: Physicochemical properties and RAW 264.7 cells responses.					
33940104	6	39	theme	potential	1279:1287	arg1	treatment					1264:1272	UV/H2O2 treatment	1256:1272	UV/H2O2 treatment	1256:1272	Therefore, UV/H2O2 treatment is a potential way to prepare polysaccharide with better anti-inflammatory activity.					
33940104	6	39	theme	potential	1279:1287	arg1	way					1289:1291	a potential way	1277:1291	a potential way to prepare polysaccharide with better anti-inflammatory activity	1277:1356	Therefore, UV/H2O2 treatment is a potential way to prepare polysaccharide with better anti-inflammatory activity.					
33940104	0	40	from	fusiforme	65:73	arg1	polysaccharides					34:48	polysaccharides	34:48	polysaccharides from Sargassum fusiforme	34:73	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme: Physicochemical properties and RAW 264.7 cells responses.					
33940104	4	41	contain	had	921:923	arg1	polysaccharides					869:883	polysaccharides	869:883	polysaccharides after UV/H2O2 treatment for 0.5-2 h	869:919	Besides, polysaccharides after UV/H2O2 treatment for 0.5-2 h had lower toxicity than original polysaccharides in RAW 264.7 cells.					
33940104	4	41	contain	had	921:923	arg2	toxicity					931:938	lower toxicity	925:938	lower toxicity	925:938	Besides, polysaccharides after UV/H2O2 treatment for 0.5-2 h had lower toxicity than original polysaccharides in RAW 264.7 cells.					
33940104	4	42	theme	RAW	973:975	arg1	264.7 cells					977:987	RAW 264.7 cells	973:987	RAW 264.7 cells	973:987	Besides, polysaccharides after UV/H2O2 treatment for 0.5-2 h had lower toxicity than original polysaccharides in RAW 264.7 cells.					
33940104	1	43	theme	RAW	315:317	arg1	responses					331:339	RAW 264.7 cells responses	315:339	RAW 264.7 cells responses	315:339	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	5	44	theme	molecules	1088:1096	arg1	production					1098:1107	pro-inflammatory molecules production	1071:1107	pro-inflammatory molecules production (including NO, IL-1β, IL-6 and TNF-α)	1071:1145	Typically, PSF with UV/H2O2 treatment for 2 h (PSF-T2) could effectively inhibit pro-inflammatory molecules production (including NO, IL-1β, IL-6 and TNF-α), and down-regulate related genes expression (including Tlr4, Irak, Il-1β, Il-6, Il-12 and Tnf-α).					
33940104	4	45	theme	lower	925:929	arg1	toxicity					931:938	lower toxicity	925:938	lower toxicity	925:938	Besides, polysaccharides after UV/H2O2 treatment for 0.5-2 h had lower toxicity than original polysaccharides in RAW 264.7 cells.					
33940104	2	46	theme	total	560:564	arg1	sugar					566:570	total sugar	560:570	total sugar	560:570	Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides.					
33940104	1	47	theme	264.7 cells	319:329	arg1	responses					331:339	RAW 264.7 cells responses	315:339	RAW 264.7 cells responses	315:339	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	5	48	theme	UV/H2O2	1010:1016	arg1	treatment					1018:1026	UV/H2O2 treatment	1010:1026	UV/H2O2 treatment	1010:1026	Typically, PSF with UV/H2O2 treatment for 2 h (PSF-T2) could effectively inhibit pro-inflammatory molecules production (including NO, IL-1β, IL-6 and TNF-α), and down-regulate related genes expression (including Tlr4, Irak, Il-1β, Il-6, Il-12 and Tnf-α).					
33940104	3	49	theme	monosaccharide	735:748	arg1	types					750:754	the monosaccharide types	731:754	the monosaccharide types of original polysaccharides	731:782	In addition, UV/H2O2 treatment did not change the monosaccharide types of original polysaccharides, but it could change its monosaccharide composition and surface morphology.					
33940104	1	50	theme	few	144:146	arg1	studies					148:154	few studies	144:154	few studies on seaweed polysaccharides with UV/H2O2 treatment	144:204	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	51	theme	study	226:230	arg1	aim					214:216	the aim	210:216	the aim of this study	210:230	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	2	52	theme	original	659:666	arg1	polysaccharides					668:682	original polysaccharides	659:682	original polysaccharides	659:682	Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides.					
33940104	1	53	from	responses	331:339	arg1	fusiforme					375:383	Sargassum fusiforme	365:383	Sargassum fusiforme (PSF)	365:389	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	1	53	from	responses	331:339	arg1	PSF					386:388	PSF	386:388	PSF	386:388	There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF).					
33940104	2	54	from	sugar	437:441	arg1	PSF					458:460	PSF	458:460	PSF	458:460	Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides.					
33940104	0	55	theme	UV/H2O2	13:19	arg1	treatment					21:29	UV/H2O2 treatment	13:29	UV/H2O2 treatment	13:29	Influence of UV/H2O2 treatment on polysaccharides from Sargassum fusiforme: Physicochemical properties and RAW 264.7 cells responses.					
33940104	5	56	theme	genes	1174:1178	arg1	expression					1180:1189	related genes expression	1166:1189	related genes expression (including Tlr4, Irak, Il-1β, Il-6, Il-12 and Tnf-α)	1166:1242	Typically, PSF with UV/H2O2 treatment for 2 h (PSF-T2) could effectively inhibit pro-inflammatory molecules production (including NO, IL-1β, IL-6 and TNF-α), and down-regulate related genes expression (including Tlr4, Irak, Il-1β, Il-6, Il-12 and Tnf-α).					
33498300	0	0	theme	Virus	81:85	arg1	Protein					64:70	the Envelope Protein	51:70	the Envelope Protein of Alfuy Virus	51:85	Novel Flavivirus Attenuation Markers Identified in the Envelope Protein of Alfuy Virus.					
33498300	5	1	theme	ALFV	711:714	arg1	strains					716:722	ALFV strains	711:722	ALFV strains	711:722	Sequence comparisons between ALFV strains and structural modelling incriminated a positively-charged residue (K327) in ALFV3929 as a GAG-binding motif.					
33498300	7	2	theme	E327/E273-277	1302:1314	arg1	combination					1316:1326	the E327/E273-277 combination	1298:1326	the E327/E273-277 combination most dominant	1298:1340	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	3	3	theme	ALFV3929	437:444	arg1	binding					446:452	ALFV3929 binding	437:452	ALFV3929 binding to glycosaminoglycans (GAG), a known mechanism of flaviviruses attenuation	437:527	To further determine the mechanisms of attenuation we assessed ALFV3929 binding to glycosaminoglycans (GAG), a known mechanism of flaviviruses attenuation.					
33498300	2	4	from	protein	253:259	arg1	lack					339:342	lack	339:342	lack of glycosylation at E154-156	339:371	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	4	from	protein	253:259	arg1	region					317:322	the hinge region	307:322	the hinge region (E273-277)	307:333	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	4	from	protein	253:259	arg1	markers					210:216	markers	210:216	markers	210:216	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	6	5	theme	described	934:942	arg1	virus					953:957	a previously described chimeric virus	921:957	a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME)	921:1039	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	0	6	theme	Alfuy	75:79	arg1	Virus					81:85	Alfuy Virus	75:85	Alfuy Virus	75:85	Novel Flavivirus Attenuation Markers Identified in the Envelope Protein of Alfuy Virus.					
33498300	2	7	gly	glycosylation	347:359	arg1	E154-156					364:371	E154-156	364:371	E154-156	364:371	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	7	gly	glycosylation	347:359	arg2	E154-156					364:371	E154-156	364:371	E154-156	364:371	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	6	8	theme	MVEV	1019:1022	arg1	backbone					1007:1014	the backbone	1003:1014	the backbone of MVEV (MVEV/ALFV-prME)	1003:1039	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	7	9	theme	ALFV3929	1155:1162	arg1	E154-156					1143:1150	E154-156	1143:1150	E154-156	1143:1150	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	7	9	theme	ALFV3929	1155:1162	arg1	E273-277					1130:1137	E273-277	1130:1137	E273-277	1130:1137	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	7	9	theme	ALFV3929	1155:1162	arg1	E327					1124:1127	E327	1124:1127	E327	1124:1127	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	6	10	from	role	1054:1057	arg1	binding					1084:1090	enhanced GAG binding	1071:1090	enhanced GAG binding	1071:1090	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	6	11	theme	residue	855:861	arg1	Substitution					834:845	Substitution	834:845	Substitution	834:845	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	7	12	from	E273-277	1130:1137	arg1	residues					1112:1119	the wild type residues	1098:1119	the wild type residues at E327, E273-277 and E154-156 of ALFV3929	1098:1162	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	7	13	theme	type	1107:1110	arg1	residues					1112:1119	the wild type residues	1098:1119	the wild type residues at E327, E273-277 and E154-156 of ALFV3929	1098:1162	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	7	14	theme	wild	1102:1105	arg1	type					1107:1110	wild type	1102:1110	the wild type residues at E327, E273-277 and E154-156 of ALFV3929	1098:1162	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	5	15	from	residue	783:789	arg1	ALFV3929					801:808	ALFV3929	801:808	ALFV3929	801:808	Sequence comparisons between ALFV strains and structural modelling incriminated a positively-charged residue (K327) in ALFV3929 as a GAG-binding motif.					
33498300	6	16	theme	GAG	1080:1082	arg1	binding					1084:1090	enhanced GAG binding	1071:1090	enhanced GAG binding	1071:1090	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	2	17	theme	E	250:250	arg1	protein					253:259	the envelope (E) protein	236:259	the envelope (E) protein of the prototype strain (ALFV3929)	236:294	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	18	theme	strain	278:283	arg1	protein					253:259	the envelope (E) protein	236:259	the envelope (E) protein of the prototype strain (ALFV3929)	236:294	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	8	19	theme	new	1425:1427	arg1	insights					1429:1436	new insights	1425:1436	new insights for the rational design of attenuated flavivirus vaccines	1425:1494	These data demonstrate that attenuation of ALFV3929 is multifactorial and provide new insights for the rational design of attenuated flavivirus vaccines.					
33498300	7	20	from	E154-156	1143:1150	arg1	residues					1112:1119	the wild type residues	1098:1119	the wild type residues at E327, E273-277 and E154-156 of ALFV3929	1098:1162	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	6	21	contain	containing	959:968	arg1	virus					953:957	a previously described chimeric virus	921:957	a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME)	921:1039	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	6	21	contain	containing	959:968	arg2	genes					982:986	the prM & E genes	970:986	the prM & E genes of ALFV3929	970:998	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	5	22	theme	GAG-binding	815:825	arg1	residue					783:789	a positively-charged residue	762:789	a positively-charged residue (K327) in ALFV3929	762:808	Sequence comparisons between ALFV strains and structural modelling incriminated a positively-charged residue (K327) in ALFV3929 as a GAG-binding motif.					
33498300	5	22	theme	GAG-binding	815:825	arg1	motif					827:831	a GAG-binding motif	813:831	a GAG-binding motif	813:831	Sequence comparisons between ALFV strains and structural modelling incriminated a positively-charged residue (K327) in ALFV3929 as a GAG-binding motif.					
33498300	1	23	theme	Murray	144:149	arg1	encephalitis					158:169	Murray Valley encephalitis	144:169	the Murray Valley encephalitis virus (MVEV)	140:182	Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV).					
33498300	5	24	theme	Sequence	682:689	arg1	comparisons					691:701	Sequence comparisons	682:701	Sequence comparisons between ALFV strains and structural modelling	682:747	Sequence comparisons between ALFV strains and structural modelling incriminated a positively-charged residue (K327) in ALFV3929 as a GAG-binding motif.					
33498300	2	25	theme	envelope	240:247	arg1	protein					253:259	the envelope (E) protein	236:259	the envelope (E) protein of the prototype strain (ALFV3929)	236:294	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	26	from	markers	210:216	arg1	protein					253:259	the envelope (E) protein	236:259	the envelope (E) protein of the prototype strain (ALFV3929)	236:294	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	1	27	theme	Valley	151:156	arg1	encephalitis					158:169	Murray Valley encephalitis	144:169	the Murray Valley encephalitis virus (MVEV)	140:182	Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV).					
33498300	0	28	theme	Flavivirus	6:15	arg1	Markers					29:35	Novel Flavivirus Attenuation Markers	0:35	Novel Flavivirus Attenuation Markers	0:35	Novel Flavivirus Attenuation Markers Identified in the Envelope Protein of Alfuy Virus.					
33498300	2	29	theme	prototype	268:276	arg1	ALFV3929					286:293	ALFV3929	286:293	ALFV3929	286:293	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	29	theme	prototype	268:276	arg1	strain					278:283	the prototype strain	264:283	the prototype strain (ALFV3929)	264:294	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	6	30	theme	ALFV3929	991:998	arg1	genes					982:986	the prM & E genes	970:986	the prM & E genes of ALFV3929	970:998	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	1	31	theme	encephalitis	158:169	arg1	MVEV					178:181	MVEV	178:181	MVEV	178:181	Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV).					
33498300	1	31	theme	encephalitis	158:169	arg1	virus					171:175	the Murray Valley encephalitis virus	140:175	the Murray Valley encephalitis virus (MVEV)	140:182	Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV).					
33498300	0	32	theme	Novel	0:4	arg1	Markers					29:35	Novel Flavivirus Attenuation Markers	0:35	Novel Flavivirus Attenuation Markers	0:35	Novel Flavivirus Attenuation Markers Identified in the Envelope Protein of Alfuy Virus.					
33498300	7	33	from	E327	1124:1127	arg1	residues					1112:1119	the wild type residues	1098:1119	the wild type residues at E327, E273-277 and E154-156 of ALFV3929	1098:1162	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	2	34	from	attenuation	221:231	arg1	protein					253:259	the envelope (E) protein	236:259	the envelope (E) protein of the prototype strain (ALFV3929)	236:294	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	7	35	theme	dominant	1333:1340	arg1	combination					1316:1326	the E327/E273-277 combination	1298:1326	the E327/E273-277 combination most dominant	1298:1340	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	5	36	theme	positively-charged	764:781	arg1	K327					792:795	K327	792:795	K327	792:795	Sequence comparisons between ALFV strains and structural modelling incriminated a positively-charged residue (K327) in ALFV3929 as a GAG-binding motif.					
33498300	5	36	theme	positively-charged	764:781	arg1	residue					783:789	a positively-charged residue	762:789	a positively-charged residue (K327) in ALFV3929	762:808	Sequence comparisons between ALFV strains and structural modelling incriminated a positively-charged residue (K327) in ALFV3929 as a GAG-binding motif.					
33498300	5	36	theme	positively-charged	764:781	arg1	motif					827:831	a GAG-binding motif	813:831	a GAG-binding motif	813:831	Sequence comparisons between ALFV strains and structural modelling incriminated a positively-charged residue (K327) in ALFV3929 as a GAG-binding motif.					
33498300	7	37	theme	ALFV3929	1283:1290	arg1	attenuation					1268:1278	attenuation	1268:1278	attenuation of ALFV3929	1268:1290	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	2	38	theme	glycosylation	347:359	arg1	E273-277					325:332	E273-277	325:332	E273-277	325:332	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	38	theme	glycosylation	347:359	arg1	lack					339:342	lack	339:342	lack of glycosylation at E154-156	339:371	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	38	theme	glycosylation	347:359	arg1	region					317:322	the hinge region	307:322	the hinge region (E273-277)	307:333	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	0	39	theme	Attenuation	17:27	arg1	Markers					29:35	Novel Flavivirus Attenuation Markers	0:35	Novel Flavivirus Attenuation Markers	0:35	Novel Flavivirus Attenuation Markers Identified in the Envelope Protein of Alfuy Virus.					
33498300	8	40	theme	attenuated	1465:1474	arg1	vaccines					1487:1494	attenuated flavivirus vaccines	1465:1494	attenuated flavivirus vaccines	1465:1494	These data demonstrate that attenuation of ALFV3929 is multifactorial and provide new insights for the rational design of attenuated flavivirus vaccines.					
33498300	6	41	theme	enhanced	1071:1078	arg1	binding					1084:1090	enhanced GAG binding	1071:1090	enhanced GAG binding	1071:1090	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	3	42	theme	known	485:489	arg1	mechanism					491:499	a known mechanism	483:499	a known mechanism of flaviviruses attenuation	483:527	To further determine the mechanisms of attenuation we assessed ALFV3929 binding to glycosaminoglycans (GAG), a known mechanism of flaviviruses attenuation.					
33498300	3	42	theme	known	485:489	arg1	glycosaminoglycans					457:474	glycosaminoglycans	457:474	glycosaminoglycans (GAG)	457:480	To further determine the mechanisms of attenuation we assessed ALFV3929 binding to glycosaminoglycans (GAG), a known mechanism of flaviviruses attenuation.					
33498300	6	43	theme	E	980:980	arg1	genes					982:986	the prM & E genes	970:986	the prM & E genes of ALFV3929	970:998	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	7	44	theme	virulent	1215:1222	arg1	MVEV					1224:1227	virulent MVEV	1215:1227	virulent MVEV	1215:1227	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	6	45	theme	&	978:978	arg1	genes					982:986	the prM & E genes	970:986	the prM & E genes of ALFV3929	970:998	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	3	46	dep	flaviviruses	504:515	arg1	attenuation					517:527	attenuation	517:527	flaviviruses attenuation	504:527	To further determine the mechanisms of attenuation we assessed ALFV3929 binding to glycosaminoglycans (GAG), a known mechanism of flaviviruses attenuation.					
33498300	8	47	theme	vaccines	1487:1494	arg1	design					1455:1460	the rational design	1442:1460	the rational design of attenuated flavivirus vaccines	1442:1494	These data demonstrate that attenuation of ALFV3929 is multifactorial and provide new insights for the rational design of attenuated flavivirus vaccines.					
33498300	5	48	theme	structural	728:737	arg1	modelling					739:747	structural modelling	728:747	structural modelling	728:747	Sequence comparisons between ALFV strains and structural modelling incriminated a positively-charged residue (K327) in ALFV3929 as a GAG-binding motif.					
33498300	4	49	theme	GAG-rich	576:583	arg1	cells					585:589	GAG-rich cells	576:589	GAG-rich cells	576:589	Indeed, ALFV3929 exhibited reduced binding to GAG-rich cells in the presence of heparin; however, low-passage ALFV isolates were relatively unaffected.					
33498300	0	50	theme	Envelope	55:62	arg1	Protein					64:70	the Envelope Protein	51:70	the Envelope Protein of Alfuy Virus	51:85	Novel Flavivirus Attenuation Markers Identified in the Envelope Protein of Alfuy Virus.					
33498300	8	51	theme	flavivirus	1476:1485	arg1	vaccines					1487:1494	attenuated flavivirus vaccines	1465:1494	attenuated flavivirus vaccines	1465:1494	These data demonstrate that attenuation of ALFV3929 is multifactorial and provide new insights for the rational design of attenuated flavivirus vaccines.					
33498300	6	52	from	residue	894:900	arg1	L					911:911	L	911:911	L	911:911	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	6	52	from	residue	894:900	arg1	MVEV					905:908	MVEV	905:908	MVEV (L)	905:912	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	8	53	theme	rational	1446:1453	arg1	design					1455:1460	the rational design	1442:1460	the rational design of attenuated flavivirus vaccines	1442:1494	These data demonstrate that attenuation of ALFV3929 is multifactorial and provide new insights for the rational design of attenuated flavivirus vaccines.					
33498300	3	54	theme	flaviviruses	504:515	arg1	mechanism					491:499	a known mechanism	483:499	a known mechanism of flaviviruses attenuation	483:527	To further determine the mechanisms of attenuation we assessed ALFV3929 binding to glycosaminoglycans (GAG), a known mechanism of flaviviruses attenuation.					
33498300	3	54	theme	flaviviruses	504:515	arg1	glycosaminoglycans					457:474	glycosaminoglycans	457:474	glycosaminoglycans (GAG)	457:480	To further determine the mechanisms of attenuation we assessed ALFV3929 binding to glycosaminoglycans (GAG), a known mechanism of flaviviruses attenuation.					
33498300	2	55	from	region	317:322	arg1	E154-156					364:371	E154-156	364:371	E154-156	364:371	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	4	56	theme	ALFV	640:643	arg1	isolates					645:652	low-passage ALFV isolates	628:652	low-passage ALFV isolates	628:652	Indeed, ALFV3929 exhibited reduced binding to GAG-rich cells in the presence of heparin; however, low-passage ALFV isolates were relatively unaffected.					
33498300	6	57	theme	corresponding	870:882	arg1	residue					894:900	the corresponding uncharged residue	866:900	the corresponding uncharged residue in MVEV (L)	866:912	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	4	58	theme	low-passage	628:638	arg1	isolates					645:652	low-passage ALFV isolates	628:652	low-passage ALFV isolates	628:652	Indeed, ALFV3929 exhibited reduced binding to GAG-rich cells in the presence of heparin; however, low-passage ALFV isolates were relatively unaffected.					
33498300	6	59	theme	uncharged	884:892	arg1	residue					894:900	the corresponding uncharged residue	866:900	the corresponding uncharged residue in MVEV (L)	866:912	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	4	60	theme	heparin	610:616	arg1	presence					598:605	the presence	594:605	the presence of heparin	594:616	Indeed, ALFV3929 exhibited reduced binding to GAG-rich cells in the presence of heparin; however, low-passage ALFV isolates were relatively unaffected.					
33498300	3	61	dep	assessed	428:435	arg1	determine					385:393	determine	385:393	To further determine the mechanisms of attenuation	374:423	To further determine the mechanisms of attenuation we assessed ALFV3929 binding to glycosaminoglycans (GAG), a known mechanism of flaviviruses attenuation.					
33498300	8	62	theme	ALFV3929	1386:1393	arg1	attenuation					1371:1381	attenuation	1371:1381	attenuation of ALFV3929	1371:1393	These data demonstrate that attenuation of ALFV3929 is multifactorial and provide new insights for the rational design of attenuated flavivirus vaccines.					
33498300	2	63	from	lack	339:342	arg1	E154-156					364:371	E154-156	364:371	E154-156	364:371	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	1	64	theme	attenuated	107:116	arg1	Alfuy					88:92	Alfuy	88:92	Alfuy (ALFV)	88:99	Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV).					
33498300	1	64	theme	attenuated	107:116	arg1	flavivirus					118:127	an attenuated flavivirus	104:127	an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV)	104:182	Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV).					
33498300	6	65	theme	prM	974:976	arg1	genes					982:986	the prM & E genes	970:986	the prM & E genes of ALFV3929	970:998	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
33498300	2	66	theme	attenuation	221:231	arg1	lack					339:342	lack	339:342	lack of glycosylation at E154-156	339:371	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	66	theme	attenuation	221:231	arg1	region					317:322	the hinge region	307:322	the hinge region (E273-277)	307:333	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	66	theme	attenuation	221:231	arg1	markers					210:216	markers	210:216	markers	210:216	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	7	67	theme	corresponding	1187:1199	arg1	residues					1201:1208	the corresponding residues	1183:1208	the corresponding residues from virulent MVEV	1183:1227	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	2	68	theme	hinge	311:315	arg1	E273-277					325:332	E273-277	325:332	E273-277	325:332	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	68	theme	hinge	311:315	arg1	region					317:322	the hinge region	307:322	the hinge region (E273-277)	307:333	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	3	69	theme	attenuation	413:423	arg1	mechanisms					399:408	the mechanisms	395:408	the mechanisms of attenuation	395:423	To further determine the mechanisms of attenuation we assessed ALFV3929 binding to glycosaminoglycans (GAG), a known mechanism of flaviviruses attenuation.					
33498300	4	70	theme	reduced	557:563	arg1	binding					565:571	reduced binding	557:571	reduced binding to GAG-rich cells	557:589	Indeed, ALFV3929 exhibited reduced binding to GAG-rich cells in the presence of heparin; however, low-passage ALFV isolates were relatively unaffected.					
33498300	2	71	from	E154-156	364:371	arg1	E273-277					325:332	E273-277	325:332	E273-277	325:332	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	71	from	E154-156	364:371	arg1	lack					339:342	lack	339:342	lack of glycosylation at E154-156	339:371	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	71	from	E154-156	364:371	arg1	region					317:322	the hinge region	307:322	the hinge region (E273-277)	307:333	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	2	71	from	E154-156	364:371	arg1	glycosylation					347:359	glycosylation	347:359	glycosylation at E154-156	347:371	We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273-277) and lack of glycosylation at E154-156.					
33498300	7	72	from	MVEV	1224:1227	arg1	residues					1201:1208	the corresponding residues	1183:1208	the corresponding residues from virulent MVEV	1183:1227	When the wild type residues at E327, E273-277 and E154-156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273-277 combination most dominant.					
33498300	1	73	theme	related	129:135	arg1	Alfuy					88:92	Alfuy	88:92	Alfuy (ALFV)	88:99	Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV).					
33498300	1	73	theme	related	129:135	arg1	flavivirus					118:127	an attenuated flavivirus	104:127	an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV)	104:182	Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV).					
33498300	6	74	theme	chimeric	944:951	arg1	virus					953:957	a previously described chimeric virus	921:957	a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME)	921:1039	Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding.					
32061693	0	0	theme	stepwise	113:120	arg1	precipitation					130:142	stepwise ethanol precipitation	113:142	stepwise ethanol precipitation from Caulerpa lentillifera	113:169	Physicochemical characterization and bile acid-binding capacity of water-extract polysaccharides fractionated by stepwise ethanol precipitation from Caulerpa lentillifera.					
32061693	6	1	theme	ethanol	954:960	arg1	concentration					962:974	the ethanol concentration	950:974	the ethanol concentration	950:974	The WCLPs are high-molecular-weight sulfated heteropolysaccharides, and the ethanol concentration significantly influenced the physicochemical properties of the extract.					
32061693	1	2	theme	lentillifera	205:216	arg1	polysaccharides					218:232	water-extracted Caulerpa lentillifera polysaccharides	180:232	water-extracted Caulerpa lentillifera polysaccharides	180:232	Herein, water-extracted Caulerpa lentillifera polysaccharides were structurally characterized and their bile acid-binding capacities were investigated.					
32061693	2	3	theme	ethanol	423:429	arg1	concentrations					431:444	ethanol concentrations	423:444	ethanol concentrations of 25%, 40%, 55%, 70%, and 85%	423:475	WCLP-25, WCLP-40, WCLP-55, WCLP-70, and WCLP-85 were obtained by graded ethanol precipitation with ethanol concentrations of 25%, 40%, 55%, 70%, and 85%.					
32061693	7	4	theme	sulfate	1239:1245	arg1	contents					1247:1254	sulfate contents	1239:1254	sulfate contents	1239:1254	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	0	5	theme	ethanol	122:128	arg1	precipitation					130:142	stepwise ethanol precipitation	113:142	stepwise ethanol precipitation from Caulerpa lentillifera	113:169	Physicochemical characterization and bile acid-binding capacity of water-extract polysaccharides fractionated by stepwise ethanol precipitation from Caulerpa lentillifera.					
32061693	5	6	dep	in	819:820	arg1	vitro					822:826	vitro	822:826	vitro	822:826	The hypolipidaemic effects of the WCLPs were assessed with in vitro simulated bile acid-binding capacity experiments.					
32061693	5	6	dep	in	819:820	arg1	simulated					828:836	simulated	828:836	simulated	828:836	The hypolipidaemic effects of the WCLPs were assessed with in vitro simulated bile acid-binding capacity experiments.					
32061693	4	7	theme	electron	712:719	arg1	SEM					733:735	SEM	733:735	SEM	733:735	Their infrared spectra, thermogravimetric curves, and scanning electron microscopy (SEM) images were acquired.					
32061693	4	7	theme	electron	712:719	arg1	microscopy					721:730	scanning electron microscopy	703:730	scanning electron microscopy (SEM) images	703:743	Their infrared spectra, thermogravimetric curves, and scanning electron microscopy (SEM) images were acquired.					
32061693	5	8	theme	capacity	856:863	arg1	experiments					865:875	in vitro simulated bile acid-binding capacity experiments	819:875	in vitro simulated bile acid-binding capacity experiments	819:875	The hypolipidaemic effects of the WCLPs were assessed with in vitro simulated bile acid-binding capacity experiments.					
32061693	0	9	theme	Caulerpa	149:156	arg1	lentillifera					158:169	Caulerpa lentillifera	149:169	Caulerpa lentillifera	149:169	Physicochemical characterization and bile acid-binding capacity of water-extract polysaccharides fractionated by stepwise ethanol precipitation from Caulerpa lentillifera.					
32061693	5	10	theme	acid-binding	843:854	arg1	capacity					856:863	bile acid-binding capacity	838:863	in vitro simulated bile acid-binding capacity experiments	819:875	The hypolipidaemic effects of the WCLPs were assessed with in vitro simulated bile acid-binding capacity experiments.					
32061693	2	11	theme	%	461:461	arg1	concentrations					431:444	ethanol concentrations	423:444	ethanol concentrations of 25%, 40%, 55%, 70%, and 85%	423:475	WCLP-25, WCLP-40, WCLP-55, WCLP-70, and WCLP-85 were obtained by graded ethanol precipitation with ethanol concentrations of 25%, 40%, 55%, 70%, and 85%.					
32061693	3	12	theme	sulfate	527:533	arg1	contents					535:542	sulfate contents	527:542	sulfate contents	527:542	The total carbohydrate, protein, uronic acid and sulfate contents as well as the monosaccharide composition, molecular weight, and rheological properties were determined.					
32061693	2	13	theme	%	456:456	arg1	concentrations					431:444	ethanol concentrations	423:444	ethanol concentrations of 25%, 40%, 55%, 70%, and 85%	423:475	WCLP-25, WCLP-40, WCLP-55, WCLP-70, and WCLP-85 were obtained by graded ethanol precipitation with ethanol concentrations of 25%, 40%, 55%, 70%, and 85%.					
32061693	6	14	theme	sulfated	914:921	arg1	heteropolysaccharides					923:943	high-molecular-weight sulfated heteropolysaccharides	892:943	high-molecular-weight sulfated heteropolysaccharides	892:943	The WCLPs are high-molecular-weight sulfated heteropolysaccharides, and the ethanol concentration significantly influenced the physicochemical properties of the extract.					
32061693	6	14	theme	sulfated	914:921	arg1	WCLPs					882:886	The WCLPs	878:886	The WCLPs	878:886	The WCLPs are high-molecular-weight sulfated heteropolysaccharides, and the ethanol concentration significantly influenced the physicochemical properties of the extract.					
32061693	5	15	theme	in	819:820	arg1	experiments					865:875	in vitro simulated bile acid-binding capacity experiments	819:875	in vitro simulated bile acid-binding capacity experiments	819:875	The hypolipidaemic effects of the WCLPs were assessed with in vitro simulated bile acid-binding capacity experiments.					
32061693	6	16	theme	high-molecular-weight	892:912	arg1	heteropolysaccharides					923:943	high-molecular-weight sulfated heteropolysaccharides	892:943	high-molecular-weight sulfated heteropolysaccharides	892:943	The WCLPs are high-molecular-weight sulfated heteropolysaccharides, and the ethanol concentration significantly influenced the physicochemical properties of the extract.					
32061693	6	16	theme	high-molecular-weight	892:912	arg1	WCLPs					882:886	The WCLPs	878:886	The WCLPs	878:886	The WCLPs are high-molecular-weight sulfated heteropolysaccharides, and the ethanol concentration significantly influenced the physicochemical properties of the extract.					
32061693	7	17	theme	other	1148:1152	arg1	due					1188:1190	due	1188:1190	due	1188:1190	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	7	17	theme	other	1148:1152	arg1	samples					1166:1172	the other tested WCLP samples	1144:1172	the other tested WCLP samples	1144:1172	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	3	18	theme	monosaccharide	559:572	arg1	composition					574:584	the monosaccharide composition	555:584	the monosaccharide composition	555:584	The total carbohydrate, protein, uronic acid and sulfate contents as well as the monosaccharide composition, molecular weight, and rheological properties were determined.					
32061693	7	19	theme	sheet	1304:1308	arg1	structure					1310:1318	a larger sheet structure	1295:1318	a larger sheet structure based on SEM	1295:1331	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	0	20	theme	Physicochemical	0:14	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization and bile acid-binding capacity of water-extract polysaccharides fractionated by stepwise ethanol precipitation from Caulerpa lentillifera.					
32061693	6	21	theme	extract	1039:1045	arg1	properties					1021:1030	the physicochemical properties	1001:1030	the physicochemical properties of the extract	1001:1045	The WCLPs are high-molecular-weight sulfated heteropolysaccharides, and the ethanol concentration significantly influenced the physicochemical properties of the extract.					
32061693	7	22	theme	tested	1154:1159	arg1	due					1188:1190	due	1188:1190	due	1188:1190	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	7	22	theme	tested	1154:1159	arg1	samples					1166:1172	the other tested WCLP samples	1144:1172	the other tested WCLP samples	1144:1172	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	7	23	theme	WCLP	1161:1164	arg1	due					1188:1190	due	1188:1190	due	1188:1190	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	7	23	theme	WCLP	1161:1164	arg1	samples					1166:1172	the other tested WCLP samples	1144:1172	the other tested WCLP samples	1144:1172	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	2	24	theme	%	451:451	arg1	concentrations					431:444	ethanol concentrations	423:444	ethanol concentrations of 25%, 40%, 55%, 70%, and 85%	423:475	WCLP-25, WCLP-40, WCLP-55, WCLP-70, and WCLP-85 were obtained by graded ethanol precipitation with ethanol concentrations of 25%, 40%, 55%, 70%, and 85%.					
32061693	0	25	theme	bile	37:40	arg1	capacity					55:62	bile acid-binding capacity	37:62	bile acid-binding capacity	37:62	Physicochemical characterization and bile acid-binding capacity of water-extract polysaccharides fractionated by stepwise ethanol precipitation from Caulerpa lentillifera.					
32061693	5	26	theme	bile	838:841	arg1	capacity					856:863	bile acid-binding capacity	838:863	in vitro simulated bile acid-binding capacity experiments	819:875	The hypolipidaemic effects of the WCLPs were assessed with in vitro simulated bile acid-binding capacity experiments.					
32061693	8	27	theme	functional	1391:1400	arg1	foods					1402:1406	functional foods	1391:1406	functional foods	1391:1406	This study will broaden the sources of raw materials for functional foods and provide a reference for the scientific use of C. lentillifera.					
32061693	8	28	theme	materials	1377:1385	arg1	sources					1362:1368	the sources	1358:1368	the sources of raw materials for functional foods	1358:1406	This study will broaden the sources of raw materials for functional foods and provide a reference for the scientific use of C. lentillifera.					
32061693	8	29	theme	lentillifera	1461:1472	arg1	use					1451:1453	the scientific use	1436:1453	the scientific use of C. lentillifera	1436:1472	This study will broaden the sources of raw materials for functional foods and provide a reference for the scientific use of C. lentillifera.					
32061693	3	30	theme	uronic	511:516	arg1	acid					518:521	uronic acid	511:521	uronic acid	511:521	The total carbohydrate, protein, uronic acid and sulfate contents as well as the monosaccharide composition, molecular weight, and rheological properties were determined.					
32061693	7	31	theme	higher	1201:1206	arg1	sugar					1216:1220	their higher neutral sugar	1195:1220	their higher neutral sugar	1195:1220	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	1	32	theme	bile	276:279	arg1	capacities					294:303	their bile acid-binding capacities	270:303	their bile acid-binding capacities	270:303	Herein, water-extracted Caulerpa lentillifera polysaccharides were structurally characterized and their bile acid-binding capacities were investigated.					
32061693	4	33	theme	infrared	655:662	arg1	spectra					664:670	Their infrared spectra	649:670	Their infrared spectra	649:670	Their infrared spectra, thermogravimetric curves, and scanning electron microscopy (SEM) images were acquired.					
32061693	4	34	theme	thermogravimetric	673:689	arg1	curves					691:696	thermogravimetric curves	673:696	thermogravimetric curves	673:696	Their infrared spectra, thermogravimetric curves, and scanning electron microscopy (SEM) images were acquired.					
32061693	1	35	theme	acid-binding	281:292	arg1	capacities					294:303	their bile acid-binding capacities	270:303	their bile acid-binding capacities	270:303	Herein, water-extracted Caulerpa lentillifera polysaccharides were structurally characterized and their bile acid-binding capacities were investigated.					
32061693	0	36	theme	acid-binding	42:53	arg1	capacity					55:62	bile acid-binding capacity	37:62	bile acid-binding capacity	37:62	Physicochemical characterization and bile acid-binding capacity of water-extract polysaccharides fractionated by stepwise ethanol precipitation from Caulerpa lentillifera.					
32061693	6	37	theme	physicochemical	1005:1019	arg1	properties					1021:1030	the physicochemical properties	1001:1030	the physicochemical properties of the extract	1001:1045	The WCLPs are high-molecular-weight sulfated heteropolysaccharides, and the ethanol concentration significantly influenced the physicochemical properties of the extract.					
32061693	5	38	theme	WCLPs	794:798	arg1	effects					779:785	The hypolipidaemic effects	760:785	The hypolipidaemic effects of the WCLPs	760:798	The hypolipidaemic effects of the WCLPs were assessed with in vitro simulated bile acid-binding capacity experiments.					
32061693	2	39	theme	%	466:466	arg1	concentrations					431:444	ethanol concentrations	423:444	ethanol concentrations of 25%, 40%, 55%, 70%, and 85%	423:475	WCLP-25, WCLP-40, WCLP-55, WCLP-70, and WCLP-85 were obtained by graded ethanol precipitation with ethanol concentrations of 25%, 40%, 55%, 70%, and 85%.					
32061693	7	40	theme	WCLP-55	1084:1090	arg1	higher					1123:1128	higher	1123:1128	higher	1123:1128	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	7	40	theme	WCLP-55	1084:1090	arg1	capacities					1070:1079	The bile acid-binding capacities	1048:1079	The bile acid-binding capacities of WCLP-55 and WCLP-70	1048:1102	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	2	41	theme	%	475:475	arg1	concentrations					431:444	ethanol concentrations	423:444	ethanol concentrations of 25%, 40%, 55%, 70%, and 85%	423:475	WCLP-25, WCLP-40, WCLP-55, WCLP-70, and WCLP-85 were obtained by graded ethanol precipitation with ethanol concentrations of 25%, 40%, 55%, 70%, and 85%.					
32061693	0	42	theme	polysaccharides	81:95	arg1	capacity					55:62	bile acid-binding capacity	37:62	bile acid-binding capacity	37:62	Physicochemical characterization and bile acid-binding capacity of water-extract polysaccharides fractionated by stepwise ethanol precipitation from Caulerpa lentillifera.					
32061693	0	42	theme	polysaccharides	81:95	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization and bile acid-binding capacity of water-extract polysaccharides fractionated by stepwise ethanol precipitation from Caulerpa lentillifera.					
32061693	7	43	theme	higher	1272:1277	arg1	viscosities					1279:1289	their higher viscosities	1266:1289	their higher viscosities	1266:1289	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	3	44	theme	molecular	587:595	arg1	weight					597:602	molecular weight	587:602	molecular weight	587:602	The total carbohydrate, protein, uronic acid and sulfate contents as well as the monosaccharide composition, molecular weight, and rheological properties were determined.					
32061693	4	45	theme	microscopy	721:730	arg1	images					738:743	scanning electron microscopy (SEM) images	703:743	scanning electron microscopy (SEM) images	703:743	Their infrared spectra, thermogravimetric curves, and scanning electron microscopy (SEM) images were acquired.					
32061693	7	46	theme	neutral	1208:1214	arg1	sugar					1216:1220	their higher neutral sugar	1195:1220	their higher neutral sugar	1195:1220	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	7	47	theme	WCLP-70	1096:1102	arg1	higher					1123:1128	higher	1123:1128	higher	1123:1128	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	7	47	theme	WCLP-70	1096:1102	arg1	capacities					1070:1079	The bile acid-binding capacities	1048:1079	The bile acid-binding capacities of WCLP-55 and WCLP-70	1048:1102	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	8	48	theme	scientific	1440:1449	arg1	use					1451:1453	the scientific use	1436:1453	the scientific use of C. lentillifera	1436:1472	This study will broaden the sources of raw materials for functional foods and provide a reference for the scientific use of C. lentillifera.					
32061693	5	49	theme	hypolipidaemic	764:777	arg1	effects					779:785	The hypolipidaemic effects	760:785	The hypolipidaemic effects of the WCLPs	760:798	The hypolipidaemic effects of the WCLPs were assessed with in vitro simulated bile acid-binding capacity experiments.					
32061693	2	50	theme	ethanol	396:402	arg1	precipitation					404:416	graded ethanol precipitation	389:416	graded ethanol precipitation	389:416	WCLP-25, WCLP-40, WCLP-55, WCLP-70, and WCLP-85 were obtained by graded ethanol precipitation with ethanol concentrations of 25%, 40%, 55%, 70%, and 85%.					
32061693	4	51	theme	scanning	703:710	arg1	SEM					733:735	SEM	733:735	SEM	733:735	Their infrared spectra, thermogravimetric curves, and scanning electron microscopy (SEM) images were acquired.					
32061693	4	51	theme	scanning	703:710	arg1	microscopy					721:730	scanning electron microscopy	703:730	scanning electron microscopy (SEM) images	703:743	Their infrared spectra, thermogravimetric curves, and scanning electron microscopy (SEM) images were acquired.					
32061693	8	52	theme	raw	1373:1375	arg1	materials					1377:1385	raw materials	1373:1385	raw materials	1373:1385	This study will broaden the sources of raw materials for functional foods and provide a reference for the scientific use of C. lentillifera.					
32061693	7	53	theme	bile	1052:1055	arg1	higher					1123:1128	higher	1123:1128	higher	1123:1128	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	7	53	theme	bile	1052:1055	arg1	capacities					1070:1079	The bile acid-binding capacities	1048:1079	The bile acid-binding capacities of WCLP-55 and WCLP-70	1048:1102	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	2	54	theme	graded	389:394	arg1	precipitation					404:416	graded ethanol precipitation	389:416	graded ethanol precipitation	389:416	WCLP-25, WCLP-40, WCLP-55, WCLP-70, and WCLP-85 were obtained by graded ethanol precipitation with ethanol concentrations of 25%, 40%, 55%, 70%, and 85%.					
32061693	3	55	theme	total	482:486	arg1	carbohydrate					488:499	The total carbohydrate	478:499	The total carbohydrate	478:499	The total carbohydrate, protein, uronic acid and sulfate contents as well as the monosaccharide composition, molecular weight, and rheological properties were determined.					
32061693	7	56	theme	acid-binding	1057:1068	arg1	higher					1123:1128	higher	1123:1128	higher	1123:1128	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	7	56	theme	acid-binding	1057:1068	arg1	capacities					1070:1079	The bile acid-binding capacities	1048:1079	The bile acid-binding capacities of WCLP-55 and WCLP-70	1048:1102	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	1	57	theme	water-extracted	180:194	arg1	polysaccharides					218:232	water-extracted Caulerpa lentillifera polysaccharides	180:232	water-extracted Caulerpa lentillifera polysaccharides	180:232	Herein, water-extracted Caulerpa lentillifera polysaccharides were structurally characterized and their bile acid-binding capacities were investigated.					
32061693	0	58	theme	water-extract	67:79	arg1	polysaccharides					81:95	water-extract polysaccharides	67:95	water-extract polysaccharides	67:95	Physicochemical characterization and bile acid-binding capacity of water-extract polysaccharides fractionated by stepwise ethanol precipitation from Caulerpa lentillifera.					
32061693	3	59	theme	rheological	609:619	arg1	properties					621:630	rheological properties	609:630	rheological properties	609:630	The total carbohydrate, protein, uronic acid and sulfate contents as well as the monosaccharide composition, molecular weight, and rheological properties were determined.					
32061693	7	60	theme	larger	1297:1302	arg1	structure					1310:1318	a larger sheet structure	1295:1318	a larger sheet structure based on SEM	1295:1331	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	1	61	theme	Caulerpa	196:203	arg1	polysaccharides					218:232	water-extracted Caulerpa lentillifera polysaccharides	180:232	water-extracted Caulerpa lentillifera polysaccharides	180:232	Herein, water-extracted Caulerpa lentillifera polysaccharides were structurally characterized and their bile acid-binding capacities were investigated.					
32061693	7	62	theme	uronic	1223:1228	arg1	acid					1230:1233	uronic acid	1223:1233	uronic acid	1223:1233	The bile acid-binding capacities of WCLP-55 and WCLP-70 were significantly higher than those of the other tested WCLP samples, which may be due to their higher neutral sugar, uronic acid and sulfate contents or due to their higher viscosities and a larger sheet structure based on SEM.					
32061693	0	63	from	lentillifera	158:169	arg1	precipitation					130:142	stepwise ethanol precipitation	113:142	stepwise ethanol precipitation from Caulerpa lentillifera	113:169	Physicochemical characterization and bile acid-binding capacity of water-extract polysaccharides fractionated by stepwise ethanol precipitation from Caulerpa lentillifera.					
33621576	5	0	theme	Cerium	778:783	arg1	incorporation					804:816	Cerium oxide nanoparticle incorporation	778:816	Cerium oxide nanoparticle incorporation	778:816	Cerium oxide nanoparticle incorporation enhanced the antibacterial activity of chitosan-based films against Escherichia coli and Staphylococcus aureus.					
33621576	3	1	from	plasticizer	562:572	arg1	stage					590:594	the blending stage	577:594	the blending stage of film preparation	577:614	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	6	2	theme	good	1051:1054	arg1	strength					1067:1074	good mechanical strength	1051:1074	good mechanical strength	1051:1074	This composite film is proposed as packaging or coating material because of its flexibility, antibacterial efficacy, and good mechanical strength.					
33621576	0	3	theme	films	129:133	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films.					
33621576	0	3	theme	films	129:133	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films.					
33621576	1	4	theme	versatile	222:230	arg1	particles					250:258	versatile nano cerium oxide particles	222:258	versatile nano cerium oxide particles	222:258	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	4	5	dep	transform	665:673	arg1	infrared					675:682	infrared	675:682	transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA)	665:775	Characterizations of films were done by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA).					
33621576	1	6	dep	applications	177:188	arg1	the					143:145	the	143:145	the	143:145	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	3	7	used	used	483:486	arg2	HEC					474:476	HEC	474:476	HEC	474:476	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	3	7	used	used	483:486	arg2	Hydroxyethylcellulose					451:471	Hydroxyethylcellulose	451:471	Hydroxyethylcellulose (HEC)	451:477	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	6	8	theme	mechanical	1056:1065	arg1	strength					1067:1074	good mechanical strength	1051:1074	good mechanical strength	1051:1074	This composite film is proposed as packaging or coating material because of its flexibility, antibacterial efficacy, and good mechanical strength.					
33621576	2	9	theme	composite	350:358	arg1	films					360:364	Chitosan-based composite films	335:364	Chitosan-based composite films with and without cerium oxide nanoparticles (NPs)	335:414	Chitosan-based composite films with and without cerium oxide nanoparticles (NPs) were prepared by a casting method.					
33621576	1	10	theme	nano	232:235	arg1	particles					250:258	versatile nano cerium oxide particles	222:258	versatile nano cerium oxide particles	222:258	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	6	11	theme	composite	935:943	arg1	film					945:948	This composite film	930:948	This composite film	930:948	This composite film is proposed as packaging or coating material because of its flexibility, antibacterial efficacy, and good mechanical strength.					
33621576	6	11	theme	composite	935:943	arg1	packaging					965:973	packaging	965:973	packaging	965:973	This composite film is proposed as packaging or coating material because of its flexibility, antibacterial efficacy, and good mechanical strength.					
33621576	6	11	theme	composite	935:943	arg1	material					986:993	coating material	978:993	coating material	978:993	This composite film is proposed as packaging or coating material because of its flexibility, antibacterial efficacy, and good mechanical strength.					
33621576	5	12	theme	nanoparticle	791:802	arg1	incorporation					804:816	Cerium oxide nanoparticle incorporation	778:816	Cerium oxide nanoparticle incorporation	778:816	Cerium oxide nanoparticle incorporation enhanced the antibacterial activity of chitosan-based films against Escherichia coli and Staphylococcus aureus.					
33621576	2	13	theme	Chitosan-based	335:348	arg1	films					360:364	Chitosan-based composite films	335:364	Chitosan-based composite films with and without cerium oxide nanoparticles (NPs)	335:414	Chitosan-based composite films with and without cerium oxide nanoparticles (NPs) were prepared by a casting method.					
33621576	1	14	theme	cerium	237:242	arg1	particles					250:258	versatile nano cerium oxide particles	222:258	versatile nano cerium oxide particles	222:258	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	5	15	theme	antibacterial	831:843	arg1	activity					845:852	the antibacterial activity	827:852	the antibacterial activity of chitosan-based films against Escherichia coli and Staphylococcus aureus	827:927	Cerium oxide nanoparticle incorporation enhanced the antibacterial activity of chitosan-based films against Escherichia coli and Staphylococcus aureus.					
33621576	5	16	theme	oxide	785:789	arg1	incorporation					804:816	Cerium oxide nanoparticle incorporation	778:816	Cerium oxide nanoparticle incorporation	778:816	Cerium oxide nanoparticle incorporation enhanced the antibacterial activity of chitosan-based films against Escherichia coli and Staphylococcus aureus.					
33621576	2	17	theme	oxide	390:394	arg1	NPs					411:413	NPs	411:413	NPs	411:413	Chitosan-based composite films with and without cerium oxide nanoparticles (NPs) were prepared by a casting method.					
33621576	2	17	theme	oxide	390:394	arg1	nanoparticles					396:408	cerium oxide nanoparticles	383:408	cerium oxide nanoparticles (NPs)	383:414	Chitosan-based composite films with and without cerium oxide nanoparticles (NPs) were prepared by a casting method.					
33621576	1	18	theme	oxide	244:248	arg1	particles					250:258	versatile nano cerium oxide particles	222:258	versatile nano cerium oxide particles	222:258	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	6	19	theme	antibacterial	1023:1035	arg1	efficacy					1037:1044	antibacterial efficacy	1023:1044	antibacterial efficacy	1023:1044	This composite film is proposed as packaging or coating material because of its flexibility, antibacterial efficacy, and good mechanical strength.					
33621576	2	20	theme	cerium	383:388	arg1	NPs					411:413	NPs	411:413	NPs	411:413	Chitosan-based composite films with and without cerium oxide nanoparticles (NPs) were prepared by a casting method.					
33621576	2	20	theme	cerium	383:388	arg1	nanoparticles					396:408	cerium oxide nanoparticles	383:408	cerium oxide nanoparticles (NPs)	383:414	Chitosan-based composite films with and without cerium oxide nanoparticles (NPs) were prepared by a casting method.					
33621576	2	21	theme	casting	435:441	arg1	method					443:448	a casting method	433:448	a casting method	433:448	Chitosan-based composite films with and without cerium oxide nanoparticles (NPs) were prepared by a casting method.					
33621576	4	22	theme	films	638:642	arg1	Characterizations					617:633	Characterizations	617:633	Characterizations of films	617:642	Characterizations of films were done by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA).					
33621576	4	23	theme	mechanical	751:760	arg1	DMA					772:774	DMA	772:774	DMA	772:774	Characterizations of films were done by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA).					
33621576	4	23	theme	mechanical	751:760	arg1	analyzer					762:769	dynamic mechanical analyzer	743:769	dynamic mechanical analyzer (DMA)	743:775	Characterizations of films were done by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA).					
33621576	3	24	theme	blending	581:588	arg1	stage					590:594	the blending stage	577:594	the blending stage of film preparation	577:614	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	5	25	theme	films	872:876	arg1	activity					845:852	the antibacterial activity	827:852	the antibacterial activity of chitosan-based films against Escherichia coli and Staphylococcus aureus	827:927	Cerium oxide nanoparticle incorporation enhanced the antibacterial activity of chitosan-based films against Escherichia coli and Staphylococcus aureus.					
33621576	3	26	theme	film	599:602	arg1	preparation					604:614	film preparation	599:614	film preparation	599:614	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	0	27	theme	nano	50:53	arg1	films					129:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	Preparation and characterization of antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films.					
33621576	1	28	theme	particles	250:258	arg1	applications					177:188	biological applications	166:188	biological applications	166:188	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	1	28	theme	particles	250:258	arg1	properties					208:217	antibacterial properties	194:217	antibacterial properties	194:217	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	0	29	theme	antibacterial	36:48	arg1	films					129:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	Preparation and characterization of antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films.					
33621576	1	30	from	effects	265:271	arg1	films					310:314	chitosan-based films	295:314	chitosan-based films	295:314	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	3	31	theme	films	511:515	arg1	flexibility					496:506	the flexibility	492:506	the flexibility of films	492:515	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	0	32	theme	oxide/chitosan/hydroxyethylcellulose/polyethylene	62:110	arg1	films					129:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	Preparation and characterization of antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films.					
33621576	5	33	theme	chitosan-based	857:870	arg1	films					872:876	chitosan-based films	857:876	chitosan-based films	857:876	Cerium oxide nanoparticle incorporation enhanced the antibacterial activity of chitosan-based films against Escherichia coli and Staphylococcus aureus.					
33621576	3	34	theme	preparation	604:614	arg1	stage					590:594	the blending stage	577:594	the blending stage of film preparation	577:614	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	1	35	theme	increasing	155:164	arg1	applications					177:188	biological applications	166:188	biological applications	166:188	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	0	36	theme	cerium	55:60	arg1	films					129:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	Preparation and characterization of antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films.					
33621576	4	37	dep	Fourier	657:663	arg1	transform					665:673	transform	665:673	transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA)	665:775	Characterizations of films were done by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA).					
33621576	1	38	theme	biological	166:175	arg1	applications					177:188	biological applications	166:188	biological applications	166:188	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	6	39	theme	coating	978:984	arg1	film					945:948	This composite film	930:948	This composite film	930:948	This composite film is proposed as packaging or coating material because of its flexibility, antibacterial efficacy, and good mechanical strength.					
33621576	6	39	theme	coating	978:984	arg1	material					986:993	coating material	978:993	coating material	978:993	This composite film is proposed as packaging or coating material because of its flexibility, antibacterial efficacy, and good mechanical strength.					
33621576	3	40	theme	polyethylene	522:533	arg1	PEG					543:545	PEG	543:545	PEG	543:545	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	3	40	theme	polyethylene	522:533	arg1	glycol					535:540	polyethylene glycol	522:540	polyethylene glycol (PEG)	522:546	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	3	40	theme	polyethylene	522:533	arg1	plasticizer					562:572	a plasticizer	560:572	a plasticizer in the blending stage of film preparation	560:614	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	4	41	theme	dynamic	743:749	arg1	DMA					772:774	DMA	772:774	DMA	772:774	Characterizations of films were done by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA).					
33621576	4	41	theme	dynamic	743:749	arg1	analyzer					762:769	dynamic mechanical analyzer	743:769	dynamic mechanical analyzer (DMA)	743:775	Characterizations of films were done by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA).					
33621576	1	42	theme	particles	282:290	arg1	effects					265:271	the effects	261:271	the effects of these particles on chitosan-based films	261:314	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	0	43	theme	composite	119:127	arg1	films					129:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	Preparation and characterization of antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films.					
33621576	1	44	theme	antibacterial	194:206	arg1	properties					208:217	antibacterial properties	194:217	antibacterial properties	194:217	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
33621576	0	45	theme	glycol	112:117	arg1	films					129:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films	36:133	Preparation and characterization of antibacterial nano cerium oxide/chitosan/hydroxyethylcellulose/polyethylene glycol composite films.					
33621576	3	46	used	used	552:555	arg2	PEG					543:545	PEG	543:545	PEG	543:545	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	3	46	used	used	552:555	arg2	plasticizer					562:572	a plasticizer	560:572	a plasticizer in the blending stage of film preparation	560:614	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	3	46	used	used	552:555	arg2	glycol					535:540	polyethylene glycol	522:540	polyethylene glycol (PEG)	522:546	Hydroxyethylcellulose (HEC) was used for the flexibility of films, and polyethylene glycol (PEG) was used as a plasticizer in the blending stage of film preparation.					
33621576	4	47	theme	thermogravimetric	705:721	arg1	TGA					733:735	TGA	733:735	TGA	733:735	Characterizations of films were done by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA).					
33621576	4	47	theme	thermogravimetric	705:721	arg1	analyzer					723:730	thermogravimetric analyzer	705:730	thermogravimetric analyzer (TGA)	705:736	Characterizations of films were done by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analyzer (TGA), and dynamic mechanical analyzer (DMA).					
33621576	1	48	theme	chitosan-based	295:308	arg1	films					310:314	chitosan-based films	295:314	chitosan-based films	295:314	Due to the rapidly increasing biological applications and antibacterial properties of versatile nano cerium oxide particles, the effects of these particles on chitosan-based films were investigated.					
34823411	3	0	theme	electron	767:774	arg1	microscope					776:785	scanning electron microscope	758:785	scanning electron microscope	758:785	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	5	1	with	acid	991:994	arg1	ratios					1032:1037	molar ratios	1026:1037	molar ratios of 0.91:0.04:0.03:0.02, respectively	1026:1074	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	6	2	theme	average	1081:1087	arg1	weight					1099:1104	The average molecular weight	1077:1104	The average molecular weight of GLP80-1	1077:1115	The average molecular weight of GLP80-1 was determined as 1.63 × 104 Da.					
34823411	6	2	theme	average	1081:1087	arg1	1.63 × 104 Da					1135:1147	1.63 × 104 Da	1135:1147	1.63 × 104 Da	1135:1147	The average molecular weight of GLP80-1 was determined as 1.63 × 104 Da.					
34823411	3	3	dep	Fourier	702:708	arg1	transform					710:718	transform	710:718	transform infrared	710:727	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	8	4	theme	radical	1401:1407	arg1	scavenging					1409:1418	free radical scavenging	1396:1418	free radical scavenging effects	1396:1426	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	9	5	from	antioxidant	1613:1623	arg1	food					1675:1678	functional food	1664:1678	functional food	1664:1678	The data indicated that GLP and GLP80-1 had the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food.					
34823411	0	6	theme	Glehnia	106:112	arg1	littoralis					114:123	Glehnia littoralis	106:123	Glehnia littoralis	106:123	Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	7	7	dep	-linked-α-D-Glcp	1245:1260	arg1	1→4					1241:1243	1→4	1241:1243	1→4	1241:1243	The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.					
34823411	8	8	theme	RAW264.7	1507:1514	arg1	cells					1516:1520	RAW264.7 cells	1507:1520	RAW264.7 cells	1507:1520	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	4	9	theme	molecular	842:850	arg1	1.89 × 106					899:908	1.89 × 106	899:908	1.89 × 106	899:908	The molecular weight distributions of GLP were determined as 1.89 × 106 and 1.26 × 104 Da.					
34823411	4	9	theme	molecular	842:850	arg1	1.26 × 104 Da					914:926	1.26 × 104 Da	914:926	1.26 × 104 Da	914:926	The molecular weight distributions of GLP were determined as 1.89 × 106 and 1.26 × 104 Da.					
34823411	4	9	theme	molecular	842:850	arg1	distributions					859:871	The molecular weight distributions	838:871	The molecular weight distributions of GLP	838:878	The molecular weight distributions of GLP were determined as 1.89 × 106 and 1.26 × 104 Da.					
34823411	6	10	theme	molecular	1089:1097	arg1	weight					1099:1104	The average molecular weight	1077:1104	The average molecular weight of GLP80-1	1077:1115	The average molecular weight of GLP80-1 was determined as 1.63 × 104 Da.					
34823411	6	10	theme	molecular	1089:1097	arg1	1.63 × 104 Da					1135:1147	1.63 × 104 Da	1135:1147	1.63 × 104 Da	1135:1147	The average molecular weight of GLP80-1 was determined as 1.63 × 104 Da.					
34823411	7	11	theme	single	1269:1274	arg1	branch					1285:1290	a single α-D-Glcp branch	1267:1290	a single α-D-Glcp branch substituted at C-6	1267:1309	The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.					
34823411	3	12	theme	thermogravimetric	788:804	arg1	analysis					806:813	thermogravimetric analysis	788:813	thermogravimetric analysis	788:813	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	7	13	with	chain	1231:1235	arg1	branch					1285:1290	a single α-D-Glcp branch	1267:1290	a single α-D-Glcp branch substituted at C-6	1267:1309	The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.					
34823411	4	14	theme	GLP	876:878	arg1	1.89 × 106					899:908	1.89 × 106	899:908	1.89 × 106	899:908	The molecular weight distributions of GLP were determined as 1.89 × 106 and 1.26 × 104 Da.					
34823411	4	14	theme	GLP	876:878	arg1	1.26 × 104 Da					914:926	1.26 × 104 Da	914:926	1.26 × 104 Da	914:926	The molecular weight distributions of GLP were determined as 1.89 × 106 and 1.26 × 104 Da.					
34823411	4	14	theme	GLP	876:878	arg1	distributions					859:871	The molecular weight distributions	838:871	The molecular weight distributions of GLP	838:878	The molecular weight distributions of GLP were determined as 1.89 × 106 and 1.26 × 104 Da.					
34823411	6	15	theme	GLP80-1	1109:1115	arg1	weight					1099:1104	The average molecular weight	1077:1104	The average molecular weight of GLP80-1	1077:1115	The average molecular weight of GLP80-1 was determined as 1.63 × 104 Da.					
34823411	6	15	theme	GLP80-1	1109:1115	arg1	1.63 × 104 Da					1135:1147	1.63 × 104 Da	1135:1147	1.63 × 104 Da	1135:1147	The average molecular weight of GLP80-1 was determined as 1.63 × 104 Da.					
34823411	0	16	from	activities	58:67	arg1	roots					97:101	the roots	93:101	the roots of Glehnia littoralis	93:123	Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	5	17	with	arabinose	1011:1019	arg1	ratios					1032:1037	molar ratios	1026:1037	molar ratios of 0.91:0.04:0.03:0.02, respectively	1026:1074	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	1	18	from	elucidation	165:175	arg1	roots					251:255	the roots	247:255	the roots of Glehnia littoralis	247:277	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	3	19	theme	GLP80-1	574:580	arg1	properties					538:547	The chemical properties	525:547	The chemical properties	525:547	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	3	19	theme	GLP80-1	574:580	arg1	structure					553:561	structure	553:561	structure	553:561	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	8	20	theme	biological	1327:1336	arg1	activities					1338:1347	biological activities	1327:1347	biological activities in vitro	1327:1356	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	2	21	theme	G-100	447:451	arg1	column					457:462	Sephadex G-100 gel column	438:462	Sephadex G-100 gel column	438:462	A crude polysaccharide was extracted from the roots of G. littoralis through the ultrasonic-assisted extraction and further purified by DEAE-52 cellulose and Sephadex G-100 gel column, a major polysaccharide fraction named GLP80-1 was obtained.					
34823411	0	22	theme	littoralis	114:123	arg1	roots					97:101	the roots	93:101	the roots of Glehnia littoralis	93:123	Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	8	23	theme	free	1396:1399	arg1	scavenging					1409:1418	free radical scavenging	1396:1418	free radical scavenging effects	1396:1426	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	7	24	theme	homogenous	1195:1204	arg1	structure					1154:1162	The structure	1150:1162	The structure of GLP80-1	1150:1173	The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.					
34823411	7	24	theme	homogenous	1195:1204	arg1	glucan					1206:1211	a homogenous glucan	1193:1211	a homogenous glucan	1193:1211	The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.					
34823411	4	25	theme	weight	852:857	arg1	1.89 × 106					899:908	1.89 × 106	899:908	1.89 × 106	899:908	The molecular weight distributions of GLP were determined as 1.89 × 106 and 1.26 × 104 Da.					
34823411	4	25	theme	weight	852:857	arg1	1.26 × 104 Da					914:926	1.26 × 104 Da	914:926	1.26 × 104 Da	914:926	The molecular weight distributions of GLP were determined as 1.89 × 106 and 1.26 × 104 Da.					
34823411	4	25	theme	weight	852:857	arg1	distributions					859:871	The molecular weight distributions	838:871	The molecular weight distributions of GLP	838:878	The molecular weight distributions of GLP were determined as 1.89 × 106 and 1.26 × 104 Da.					
34823411	2	26	theme	Sephadex	438:445	arg1	column					457:462	Sephadex G-100 gel column	438:462	Sephadex G-100 gel column	438:462	A crude polysaccharide was extracted from the roots of G. littoralis through the ultrasonic-assisted extraction and further purified by DEAE-52 cellulose and Sephadex G-100 gel column, a major polysaccharide fraction named GLP80-1 was obtained.					
34823411	7	27	theme	-linked-α-D-Glcp	1245:1260	arg1	chain					1231:1235	a main chain	1224:1235	a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6	1224:1309	The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.					
34823411	5	28	with	galactose	997:1005	arg1	ratios					1032:1037	molar ratios	1026:1037	molar ratios of 0.91:0.04:0.03:0.02, respectively	1026:1074	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	5	29	theme	molar	1026:1030	arg1	ratios					1032:1037	molar ratios	1026:1037	molar ratios of 0.91:0.04:0.03:0.02, respectively	1026:1074	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	3	30	dep	along	643:647	arg1	with					649:652	with	649:652	with	649:652	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	0	31	theme	Structural	0:9	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	9	32	theme	functional	1664:1673	arg1	food					1675:1678	functional food	1664:1678	functional food	1664:1678	The data indicated that GLP and GLP80-1 had the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food.					
34823411	2	33	theme	littoralis	338:347	arg1	roots					326:330	the roots	322:330	the roots of G. littoralis	322:347	A crude polysaccharide was extracted from the roots of G. littoralis through the ultrasonic-assisted extraction and further purified by DEAE-52 cellulose and Sephadex G-100 gel column, a major polysaccharide fraction named GLP80-1 was obtained.					
34823411	8	34	theme	activities	1338:1347	arg1	results					1316:1322	The results	1312:1322	The results of biological activities in vitro	1312:1356	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	0	35	theme	anti-radical	24:35	arg1	activities					58:67	anti-radical and immunomodulatory activities	24:67	anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis	24:123	Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	3	36	theme	high	654:657	arg1	chromatography					686:699	high performance gel permeation chromatography	654:699	high performance gel permeation chromatography	654:699	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	2	37	theme	G.	335:336	arg1	littoralis					338:347	G. littoralis	335:347	G. littoralis	335:347	A crude polysaccharide was extracted from the roots of G. littoralis through the ultrasonic-assisted extraction and further purified by DEAE-52 cellulose and Sephadex G-100 gel column, a major polysaccharide fraction named GLP80-1 was obtained.					
34823411	1	38	theme	polysaccharides	226:240	arg1	activities					212:221	immunomodulatory activities	195:221	immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis	195:277	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	1	38	theme	polysaccharides	226:240	arg1	elucidation					165:175	the structural elucidation	150:175	the structural elucidation	150:175	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	1	38	theme	polysaccharides	226:240	arg1	anti-radical					178:189	anti-radical	178:189	anti-radical	178:189	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	2	39	theme	major	467:471	arg1	fraction					488:495	a major polysaccharide fraction	465:495	a major polysaccharide fraction named GLP80-1	465:509	A crude polysaccharide was extracted from the roots of G. littoralis through the ultrasonic-assisted extraction and further purified by DEAE-52 cellulose and Sephadex G-100 gel column, a major polysaccharide fraction named GLP80-1 was obtained.					
34823411	8	40	dep	in	1349:1350	arg1	vitro					1352:1356	vitro	1352:1356	vitro	1352:1356	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	0	41	from	roots	97:101	arg1	polysaccharides					72:86	polysaccharides	72:86	polysaccharides from the roots of Glehnia littoralis	72:123	Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	0	41	from	roots	97:101	arg1	activities					58:67	anti-radical and immunomodulatory activities	24:67	anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis	24:123	Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	5	42	theme	0.91:0.04:0.03:0.02	1042:1060	arg1	ratios					1032:1037	molar ratios	1026:1037	molar ratios of 0.91:0.04:0.03:0.02, respectively	1026:1074	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	9	43	theme	natural	1605:1611	arg1	antioxidant					1613:1623	novel natural antioxidant	1599:1623	novel natural antioxidant	1599:1623	The data indicated that GLP and GLP80-1 had the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food.					
34823411	3	44	theme	GLP	566:568	arg1	properties					538:547	The chemical properties	525:547	The chemical properties	525:547	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	3	44	theme	GLP	566:568	arg1	structure					553:561	structure	553:561	structure	553:561	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	0	45	theme	immunomodulatory	41:56	arg1	activities					58:67	anti-radical and immunomodulatory activities	24:67	anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis	24:123	Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	3	46	theme	X-ray	819:823	arg1	diffraction					825:835	X-ray diffraction	819:835	X-ray diffraction	819:835	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	8	47	theme	mouse	1478:1482	arg1	lymphocytes					1491:1501	mouse spleen lymphocytes	1478:1501	mouse spleen lymphocytes	1478:1501	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	5	48	theme	monosaccharide	933:946	arg1	composition					948:958	The monosaccharide composition	929:958	The monosaccharide composition of GLP	929:965	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	5	48	theme	monosaccharide	933:946	arg1	glucose					971:977	glucose	971:977	glucose	971:977	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	5	49	theme	GLP	963:965	arg1	composition					948:958	The monosaccharide composition	929:958	The monosaccharide composition of GLP	929:965	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	5	49	theme	GLP	963:965	arg1	glucose					971:977	glucose	971:977	glucose	971:977	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	7	50	theme	GLP80-1	1167:1173	arg1	structure					1154:1162	The structure	1150:1162	The structure of GLP80-1	1150:1173	The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.					
34823411	7	50	theme	GLP80-1	1167:1173	arg1	glucan					1206:1211	a homogenous glucan	1193:1211	a homogenous glucan	1193:1211	The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.					
34823411	0	51	dep	activities	58:67	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	2	52	theme	gel	453:455	arg1	column					457:462	Sephadex G-100 gel column	438:462	Sephadex G-100 gel column	438:462	A crude polysaccharide was extracted from the roots of G. littoralis through the ultrasonic-assisted extraction and further purified by DEAE-52 cellulose and Sephadex G-100 gel column, a major polysaccharide fraction named GLP80-1 was obtained.					
34823411	7	53	theme	main	1226:1229	arg1	chain					1231:1235	a main chain	1224:1235	a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6	1224:1309	The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.					
34823411	7	54	theme	α-D-Glcp	1276:1283	arg1	branch					1285:1290	a single α-D-Glcp branch	1267:1290	a single α-D-Glcp branch substituted at C-6	1267:1309	The structure of GLP80-1 was deduced to be a homogenous glucan, comprised a main chain of (1→4)-linked-α-D-Glcp with a single α-D-Glcp branch substituted at C-6.					
34823411	8	55	theme	lymphocytes	1491:1501	arg1	proliferation					1461:1473	the proliferation	1457:1473	the proliferation of mouse spleen lymphocytes and RAW264.7 cells	1457:1520	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	1	56	from	roots	251:255	arg1	activities					212:221	immunomodulatory activities	195:221	immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis	195:277	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	1	56	from	roots	251:255	arg1	elucidation					165:175	the structural elucidation	150:175	the structural elucidation	150:175	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	1	56	from	roots	251:255	arg1	polysaccharides					226:240	polysaccharides	226:240	polysaccharides from the roots of Glehnia littoralis	226:277	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	1	56	from	roots	251:255	arg1	anti-radical					178:189	anti-radical	178:189	anti-radical	178:189	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	2	57	theme	polysaccharide	473:486	arg1	fraction					488:495	a major polysaccharide fraction	465:495	a major polysaccharide fraction named GLP80-1	465:509	A crude polysaccharide was extracted from the roots of G. littoralis through the ultrasonic-assisted extraction and further purified by DEAE-52 cellulose and Sephadex G-100 gel column, a major polysaccharide fraction named GLP80-1 was obtained.					
34823411	5	58	with	glucose	971:977	arg1	ratios					1032:1037	molar ratios	1026:1037	molar ratios of 0.91:0.04:0.03:0.02, respectively	1026:1074	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	3	59	theme	nuclear	730:736	arg1	resonance					747:755	nuclear magnetic resonance	730:755	nuclear magnetic resonance	730:755	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	8	60	theme	spleen	1484:1489	arg1	lymphocytes					1491:1501	mouse spleen lymphocytes	1478:1501	mouse spleen lymphocytes	1478:1501	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	8	61	theme	cells	1516:1520	arg1	proliferation					1461:1473	the proliferation	1457:1473	the proliferation of mouse spleen lymphocytes and RAW264.7 cells	1457:1520	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	9	62	theme	novel	1599:1603	arg1	antioxidant					1613:1623	novel natural antioxidant	1599:1623	novel natural antioxidant	1599:1623	The data indicated that GLP and GLP80-1 had the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food.					
34823411	3	63	theme	magnetic	738:745	arg1	resonance					747:755	nuclear magnetic resonance	730:755	nuclear magnetic resonance	730:755	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	3	64	theme	gel	671:673	arg1	chromatography					686:699	high performance gel permeation chromatography	654:699	high performance gel permeation chromatography	654:699	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	2	65	theme	ultrasonic-assisted	361:379	arg1	extraction					381:390	the ultrasonic-assisted extraction	357:390	the ultrasonic-assisted extraction	357:390	A crude polysaccharide was extracted from the roots of G. littoralis through the ultrasonic-assisted extraction and further purified by DEAE-52 cellulose and Sephadex G-100 gel column, a major polysaccharide fraction named GLP80-1 was obtained.					
34823411	3	66	theme	acid	604:607	arg1	hydrolysis					609:618	acid hydrolysis	604:618	acid hydrolysis	604:618	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	9	67	contain	had	1563:1565	arg1	GLP80-1					1555:1561	GLP80-1	1555:1561	GLP80-1	1555:1561	The data indicated that GLP and GLP80-1 had the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food.					
34823411	9	67	contain	had	1563:1565	arg2	potential					1571:1579	the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food	1567:1678	the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food	1567:1678	The data indicated that GLP and GLP80-1 had the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food.					
34823411	9	67	contain	had	1563:1565	arg1	GLP					1547:1549	GLP	1547:1549	GLP	1547:1549	The data indicated that GLP and GLP80-1 had the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food.					
34823411	3	68	theme	permeation	675:684	arg1	chromatography					686:699	high performance gel permeation chromatography	654:699	high performance gel permeation chromatography	654:699	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	1	69	from	activities	212:221	arg1	roots					251:255	the roots	247:255	the roots of Glehnia littoralis	247:277	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	3	70	theme	chemical	529:536	arg1	properties					538:547	The chemical properties	525:547	The chemical properties	525:547	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	8	71	theme	scavenging	1409:1418	arg1	effects					1420:1426	free radical scavenging effects	1396:1426	free radical scavenging effects	1396:1426	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	1	72	theme	Glehnia	260:266	arg1	littoralis					268:277	Glehnia littoralis	260:277	Glehnia littoralis	260:277	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	5	73	theme	glucuronic	980:989	arg1	acid					991:994	glucuronic acid	980:994	glucuronic acid	980:994	The monosaccharide composition of GLP was glucose, glucuronic acid, galactose and arabinose with molar ratios of 0.91:0.04:0.03:0.02, respectively.					
34823411	2	74	theme	crude	282:286	arg1	polysaccharide					288:301	A crude polysaccharide	280:301	A crude polysaccharide	280:301	A crude polysaccharide was extracted from the roots of G. littoralis through the ultrasonic-assisted extraction and further purified by DEAE-52 cellulose and Sephadex G-100 gel column, a major polysaccharide fraction named GLP80-1 was obtained.					
34823411	8	75	theme	in	1349:1350	arg1	activities					1338:1347	biological activities	1327:1347	biological activities in vitro	1327:1356	The results of biological activities in vitro showed that GLP and GLP80-1 exhibited free radical scavenging effects, and displayed promotion for the proliferation of mouse spleen lymphocytes and RAW264.7 cells.					
34823411	1	76	theme	structural	154:163	arg1	elucidation					165:175	the structural elucidation	150:175	the structural elucidation	150:175	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	1	76	theme	structural	154:163	arg1	anti-radical					178:189	anti-radical	178:189	anti-radical	178:189	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	1	77	theme	immunomodulatory	195:210	arg1	activities					212:221	immunomodulatory activities	195:221	immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis	195:277	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	1	78	theme	littoralis	268:277	arg1	roots					251:255	the roots	247:255	the roots of Glehnia littoralis	247:277	This study investigated the structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	0	79	theme	polysaccharides	72:86	arg1	activities					58:67	anti-radical and immunomodulatory activities	24:67	anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis	24:123	Structural elucidation, anti-radical and immunomodulatory activities of polysaccharides from the roots of Glehnia littoralis.					
34823411	3	80	theme	scanning	758:765	arg1	microscope					776:785	scanning electron microscope	758:785	scanning electron microscope	758:785	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	3	81	theme	performance	659:669	arg1	chromatography					686:699	high performance gel permeation chromatography	654:699	high performance gel permeation chromatography	654:699	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	3	82	theme	methylation	621:631	arg1	analysis					633:640	methylation analysis	621:640	methylation analysis	621:640	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
34823411	9	83	from	immunomodulator	1629:1643	arg1	food					1675:1678	functional food	1664:1678	functional food	1664:1678	The data indicated that GLP and GLP80-1 had the potential to be explored as novel natural antioxidant and immunomodulator for application in functional food.					
34823411	3	84	dep	transform	710:718	arg1	infrared					720:727	infrared	720:727	transform infrared	710:727	The chemical properties and structure of GLP and GLP80-1 were characterized by acid hydrolysis, methylation analysis, along with high performance gel permeation chromatography, Fourier transform infrared, nuclear magnetic resonance, scanning electron microscope, thermogravimetric analysis and X-ray diffraction.					
33432311	0	0	theme	in	89:90	arg1	fermentation					98:109	in vitro fermentation	89:109	in vitro fermentation	89:109	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	7	1	theme	similar	1491:1497	arg1	way					1499:1501	a similar way	1489:1501	a similar way	1489:1501	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	2	2	theme	linkage	546:552	arg1	-β-glucan					564:572	mixed linkage (1,3)(1,4)-β-glucan	540:572	mixed linkage (1,3)(1,4)-β-glucan (MLG)	540:578	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	2	theme	linkage	546:552	arg1	constituents					506:517	their major constituents	494:517	their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW	494:694	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	2	theme	linkage	546:552	arg1	MLG					575:577	MLG	575:577	MLG	575:577	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	1	3	from	walls	183:187	arg1	foods					235:239	foods	235:239	foods	235:239	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	4	4	theme	16S	929:931	arg1	sequencing					952:961	16S rRNA gene amplicon sequencing	929:961	16S rRNA gene amplicon sequencing	929:961	During fermentation, samples were collected for DNA extraction and 16S rRNA gene amplicon sequencing.					
33432311	3	5	theme	in	721:722	arg1	fermentation					730:741	in vitro fermentation	721:741	in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre	721:819	These samples underwent in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre to avoid prior adaptation to substrates.					
33432311	2	6	theme	major	500:504	arg1	-β-glucan					564:572	mixed linkage (1,3)(1,4)-β-glucan	540:572	mixed linkage (1,3)(1,4)-β-glucan (MLG)	540:578	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	6	theme	major	500:504	arg1	arabinoxylan					521:532	arabinoxylan	521:532	arabinoxylan (AX)	521:537	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	6	theme	major	500:504	arg1	constituents					506:517	their major constituents	494:517	their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW	494:694	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	6	theme	major	500:504	arg1	cellulose					584:592	cellulose	584:592	cellulose	584:592	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	7	7	from	walls	1480:1484	arg1	way					1499:1501	a similar way	1489:1501	a similar way	1489:1501	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	7	7	from	walls	1480:1484	arg1	able					1399:1402	able	1399:1402	able	1399:1402	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	2	8	theme	mixed	540:544	arg1	-β-glucan					564:572	mixed linkage (1,3)(1,4)-β-glucan	540:572	mixed linkage (1,3)(1,4)-β-glucan (MLG)	540:578	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	8	theme	mixed	540:544	arg1	constituents					506:517	their major constituents	494:517	their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW	494:694	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	8	theme	mixed	540:544	arg1	MLG					575:577	MLG	575:577	MLG	575:577	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	6	9	theme	end-products	1239:1250	arg1	proportions					1200:1210	proportions	1200:1210	proportions of propionate and butyrate end-products	1200:1250	Differences in proportions of propionate and butyrate end-products were associated with differences in the relative levels of genera.					
33432311	1	10	theme	Plant	172:176	arg1	walls					183:187	Plant cell walls	172:187	Plant cell walls as well as their component polysaccharides in foods	172:239	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	4	11	theme	amplicon	943:950	arg1	sequencing					952:961	16S rRNA gene amplicon sequencing	929:961	16S rRNA gene amplicon sequencing	929:961	During fermentation, samples were collected for DNA extraction and 16S rRNA gene amplicon sequencing.					
33432311	3	12	dep	in	721:722	arg1	vitro					724:728	vitro	724:728	vitro	724:728	These samples underwent in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre to avoid prior adaptation to substrates.					
33432311	2	13	dep	-β-glucan	564:572	arg1	1,4					560:562	1,4	560:562	1,4	560:562	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	3	14	with	fermentation	730:741	arg1	inoculum					757:764	a faecal inoculum	748:764	a faecal inoculum	748:764	These samples underwent in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre to avoid prior adaptation to substrates.					
33432311	2	15	theme	equivalent	663:672	arg1	Mix					658:660	Mix	658:660	Mix	658:660	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	15	theme	equivalent	663:672	arg1	polysaccharides					641:655	polysaccharides	641:655	polysaccharides (Mix) equivalent in composition to WCW	641:694	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	3	16	theme	free	787:790	arg1	diet					782:785	a diet	780:785	a diet free of cereals and soluble-fibre	780:819	These samples underwent in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre to avoid prior adaptation to substrates.					
33432311	0	17	theme	different	119:127	arg1	levels					164:169	different short chain fatty acid end-product levels	119:169	different short chain fatty acid end-product levels	119:169	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	3	18	from	pigs	771:774	arg1	fermentation					730:741	in vitro fermentation	721:741	in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre	721:819	These samples underwent in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre to avoid prior adaptation to substrates.					
33432311	1	19	theme	cell	178:181	arg1	walls					183:187	Plant cell walls	172:187	Plant cell walls as well as their component polysaccharides in foods	172:239	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	6	20	theme	butyrate	1230:1237	arg1	end-products					1239:1250	propionate and butyrate end-products	1215:1250	propionate and butyrate end-products	1215:1250	Differences in proportions of propionate and butyrate end-products were associated with differences in the relative levels of genera.					
33432311	6	21	theme	genera	1311:1316	arg1	levels					1301:1306	the relative levels	1288:1306	the relative levels of genera	1288:1316	Differences in proportions of propionate and butyrate end-products were associated with differences in the relative levels of genera.					
33432311	2	22	theme	polysaccharides	641:655	arg1	mixture					630:636	a physical mixture	619:636	a physical mixture of polysaccharides (Mix) equivalent in composition to WCW	619:694	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	23	dep	constituents	506:517	arg1	-β-glucan					564:572	mixed linkage (1,3)(1,4)-β-glucan	540:572	mixed linkage (1,3)(1,4)-β-glucan (MLG)	540:578	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	23	dep	constituents	506:517	arg1	arabinoxylan					521:532	arabinoxylan	521:532	arabinoxylan (AX)	521:537	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	23	dep	constituents	506:517	arg1	constituents					506:517	their major constituents	494:517	their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW	494:694	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	23	dep	constituents	506:517	arg1	MLG					575:577	MLG	575:577	MLG	575:577	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	23	dep	constituents	506:517	arg1	cellulose					584:592	cellulose	584:592	cellulose	584:592	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	0	24	theme	cell	6:9	arg1	walls					11:15	Wheat cell walls	0:15	Wheat cell walls	0:15	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	3	25	theme	cereals	795:801	arg1	free					787:790	free	787:790	free	787:790	These samples underwent in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre to avoid prior adaptation to substrates.					
33432311	2	26	theme	1,3	555:557	arg1	-β-glucan					564:572	mixed linkage (1,3)(1,4)-β-glucan	540:572	mixed linkage (1,3)(1,4)-β-glucan (MLG)	540:578	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	26	theme	1,3	555:557	arg1	constituents					506:517	their major constituents	494:517	their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW	494:694	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	26	theme	1,3	555:557	arg1	MLG					575:577	MLG	575:577	MLG	575:577	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	0	27	dep	in	89:90	arg1	vitro					92:96	vitro	92:96	vitro	92:96	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	0	28	theme	Wheat	0:4	arg1	walls					11:15	Wheat cell walls	0:15	Wheat cell walls	0:15	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	3	29	theme	soluble-fibre	807:819	arg1	free					787:790	free	787:790	free	787:790	These samples underwent in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre to avoid prior adaptation to substrates.					
33432311	7	30	theme	WCW	1423:1425	arg1	polysaccharides					1427:1441	diverse WCW polysaccharides	1415:1441	diverse WCW polysaccharides	1415:1441	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	0	31	theme	chain	135:139	arg1	acid					147:150	short chain fatty acid	129:150	different short chain fatty acid end-product levels	119:169	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	7	32	from	experiment	1353:1362	arg1	able					1399:1402	able	1399:1402	able	1399:1402	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	7	32	from	experiment	1353:1362	arg1	microbes					1369:1376	the microbes	1365:1376	the microbes that flourished	1365:1392	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	1	33	theme	beneficial	281:290	arg1	microbiota					302:311	a beneficial human gut microbiota	279:311	a beneficial human gut microbiota	279:311	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	0	34	theme	short	129:133	arg1	acid					147:150	short chain fatty acid	129:150	different short chain fatty acid end-product levels	119:169	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	7	35	theme	diverse	1415:1421	arg1	polysaccharides					1427:1441	diverse WCW polysaccharides	1415:1441	diverse WCW polysaccharides	1415:1441	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	2	36	theme	flour	439:443	arg1	WCW					457:459	WCW	457:459	WCW	457:459	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	36	theme	flour	439:443	arg1	walls					450:454	wheat flour cell walls	433:454	wheat flour cell walls (WCW)	433:460	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	37	theme	wheat	433:437	arg1	WCW					457:459	WCW	457:459	WCW	457:459	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	37	theme	wheat	433:437	arg1	walls					450:454	wheat flour cell walls	433:454	wheat flour cell walls (WCW)	433:460	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	7	38	theme	fermentation	1523:1534	arg1	end-products					1536:1547	different fermentation end-products	1513:1547	different fermentation end-products	1513:1547	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	2	39	theme	cell	445:448	arg1	WCW					457:459	WCW	457:459	WCW	457:459	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	39	theme	cell	445:448	arg1	walls					450:454	wheat flour cell walls	433:454	wheat flour cell walls (WCW)	433:460	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	1	40	theme	human	292:296	arg1	microbiota					302:311	a beneficial human gut microbiota	279:311	a beneficial human gut microbiota	279:311	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	0	41	theme	acid	147:150	arg1	levels					164:169	different short chain fatty acid end-product levels	119:169	different short chain fatty acid end-product levels	119:169	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	1	42	theme	cell	366:369	arg1	wall					371:374	the cell wall	362:374	the cell wall	362:374	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	7	43	theme	different	1513:1521	arg1	end-products					1536:1547	different fermentation end-products	1513:1547	different fermentation end-products	1513:1547	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	1	44	theme	component	206:214	arg1	polysaccharides					216:230	their component polysaccharides	200:230	Plant cell walls as well as their component polysaccharides in foods	172:239	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	0	45	theme	constituent	21:31	arg1	polysaccharides					33:47	constituent polysaccharides	21:47	constituent polysaccharides	21:47	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	1	46	theme	gut	298:300	arg1	microbiota					302:311	a beneficial human gut microbiota	279:311	a beneficial human gut microbiota	279:311	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	0	47	theme	fatty	141:145	arg1	acid					147:150	short chain fatty acid	129:150	different short chain fatty acid end-product levels	119:169	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	1	48	theme	wall	371:374	arg1	architecture					346:357	the architecture	342:357	the architecture of the cell wall	342:374	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	5	49	theme	microbial	1006:1014	arg1	similar					1086:1092	similar	1086:1092	similar	1086:1092	Bioinformatics analyses revealed that the microbial communities promoted during fermentation by AX, MLG, Mix and WCW were similar at the genus level, but differed from the microbiota observed for the cellulose substrate.					
33432311	5	49	theme	microbial	1006:1014	arg1	communities					1016:1026	the microbial communities	1002:1026	the microbial communities promoted during fermentation by AX, MLG, Mix and WCW	1002:1079	Bioinformatics analyses revealed that the microbial communities promoted during fermentation by AX, MLG, Mix and WCW were similar at the genus level, but differed from the microbiota observed for the cellulose substrate.					
33432311	0	50	theme	similar	56:62	arg1	profiles					75:82	similar microbiota profiles	56:82	similar microbiota profiles	56:82	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	5	51	theme	AX	1060:1061	arg1	MLG					1064:1066	MLG	1064:1066	MLG	1064:1066	Bioinformatics analyses revealed that the microbial communities promoted during fermentation by AX, MLG, Mix and WCW were similar at the genus level, but differed from the microbiota observed for the cellulose substrate.					
33432311	7	52	from	mixtures	1453:1460	arg1	able					1399:1402	able	1399:1402	able	1399:1402	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	7	52	from	mixtures	1453:1460	arg1	microbes					1369:1376	the microbes	1365:1376	the microbes that flourished	1365:1392	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	1	53	used	utilized	248:255	arg2	polysaccharides					216:230	their component polysaccharides	200:230	Plant cell walls as well as their component polysaccharides in foods	172:239	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	1	53	used	utilized	248:255	arg2	walls					183:187	Plant cell walls	172:187	Plant cell walls as well as their component polysaccharides in foods	172:239	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	1	54	from	polysaccharides	216:230	arg1	foods					235:239	foods	235:239	foods	235:239	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	6	55	theme	relative	1292:1299	arg1	levels					1301:1306	the relative levels	1288:1306	the relative levels of genera	1288:1316	Differences in proportions of propionate and butyrate end-products were associated with differences in the relative levels of genera.					
33432311	7	56	theme	cell	1475:1478	arg1	walls					1480:1484	intact cell walls	1468:1484	intact cell walls in a similar way	1468:1501	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	0	57	theme	end-product	152:162	arg1	levels					164:169	different short chain fatty acid end-product levels	119:169	different short chain fatty acid end-product levels	119:169	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	7	58	from	able	1399:1402	arg1	mixtures					1453:1460	mixtures	1453:1460	mixtures	1453:1460	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	7	58	from	able	1399:1402	arg1	experiment					1353:1362	this experiment	1348:1362	this experiment	1348:1362	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	7	58	from	able	1399:1402	arg1	walls					1480:1484	intact cell walls	1468:1484	intact cell walls in a similar way	1468:1501	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	5	59	theme	Bioinformatics	964:977	arg1	analyses					979:986	Bioinformatics analyses	964:986	Bioinformatics analyses	964:986	Bioinformatics analyses revealed that the microbial communities promoted during fermentation by AX, MLG, Mix and WCW were similar at the genus level, but differed from the microbiota observed for the cellulose substrate.					
33432311	6	60	from	Differences	1185:1195	arg1	proportions					1200:1210	proportions	1200:1210	proportions of propionate and butyrate end-products	1200:1250	Differences in proportions of propionate and butyrate end-products were associated with differences in the relative levels of genera.					
33432311	1	61	theme	gut	391:393	arg1	population					406:415	the gut microbiota population	387:415	the gut microbiota population	387:415	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	2	62	from	equivalent	663:672	arg1	composition					677:687	composition	677:687	composition to WCW	677:694	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	3	63	theme	prior	830:834	arg1	adaptation					836:845	prior adaptation	830:845	prior adaptation to substrates	830:859	These samples underwent in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre to avoid prior adaptation to substrates.					
33432311	3	64	theme	faecal	750:755	arg1	inoculum					757:764	a faecal inoculum	748:764	a faecal inoculum	748:764	These samples underwent in vitro fermentation with a faecal inoculum from pigs fed a diet free of cereals and soluble-fibre to avoid prior adaptation to substrates.					
33432311	5	65	theme	genus	1101:1105	arg1	level					1107:1111	the genus level	1097:1111	the genus level	1097:1111	Bioinformatics analyses revealed that the microbial communities promoted during fermentation by AX, MLG, Mix and WCW were similar at the genus level, but differed from the microbiota observed for the cellulose substrate.					
33432311	5	66	from	level	1107:1111	arg1	similar					1086:1092	similar	1086:1092	similar	1086:1092	Bioinformatics analyses revealed that the microbial communities promoted during fermentation by AX, MLG, Mix and WCW were similar at the genus level, but differed from the microbiota observed for the cellulose substrate.					
33432311	5	66	from	level	1107:1111	arg1	communities					1016:1026	the microbial communities	1002:1026	the microbial communities promoted during fermentation by AX, MLG, Mix and WCW	1002:1079	Bioinformatics analyses revealed that the microbial communities promoted during fermentation by AX, MLG, Mix and WCW were similar at the genus level, but differed from the microbiota observed for the cellulose substrate.					
33432311	1	67	theme	microbiota	395:404	arg1	population					406:415	the gut microbiota population	387:415	the gut microbiota population	387:415	Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the architecture of the cell wall influences the gut microbiota population.					
33432311	6	68	theme	propionate	1215:1224	arg1	end-products					1239:1250	propionate and butyrate end-products	1215:1250	propionate and butyrate end-products	1215:1250	Differences in proportions of propionate and butyrate end-products were associated with differences in the relative levels of genera.					
33432311	7	69	theme	MLG	1589:1591	arg1	polysaccharides					1604:1618	AX (propionate) or MLG (butyrate) polysaccharides	1570:1618	polysaccharides	1604:1618	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	7	69	theme	MLG	1589:1591	arg1	butyrate					1594:1601	butyrate	1594:1601	butyrate	1594:1601	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	7	70	theme	intact	1468:1473	arg1	walls					1480:1484	intact cell walls	1468:1484	intact cell walls in a similar way	1468:1501	These findings show that, in this experiment, the microbes that flourished were able to utilize diverse WCW polysaccharides alone, in mixtures or in intact cell walls in a similar way, but that different fermentation end-products were associated with AX (propionate) or MLG (butyrate) polysaccharides.					
33432311	2	71	dep	arabinoxylan	521:532	arg1	AX					535:536	AX	535:536	AX	535:536	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	4	72	theme	gene	938:941	arg1	sequencing					952:961	16S rRNA gene amplicon sequencing	929:961	16S rRNA gene amplicon sequencing	929:961	During fermentation, samples were collected for DNA extraction and 16S rRNA gene amplicon sequencing.					
33432311	2	73	from	composition	677:687	arg1	equivalent					663:672	equivalent	663:672	equivalent	663:672	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	2	74	theme	physical	621:628	arg1	mixture					630:636	a physical mixture	619:636	a physical mixture of polysaccharides (Mix) equivalent in composition to WCW	619:694	In this study, wheat flour cell walls (WCW) were isolated and compared with their major constituents - arabinoxylan (AX), mixed linkage (1,3)(1,4)-β-glucan (MLG) and cellulose - both separately and as a physical mixture of polysaccharides (Mix) equivalent in composition to WCW.					
33432311	5	75	theme	cellulose	1164:1172	arg1	substrate					1174:1182	the cellulose substrate	1160:1182	the cellulose substrate	1160:1182	Bioinformatics analyses revealed that the microbial communities promoted during fermentation by AX, MLG, Mix and WCW were similar at the genus level, but differed from the microbiota observed for the cellulose substrate.					
33432311	0	76	theme	microbiota	64:73	arg1	profiles					75:82	similar microbiota profiles	56:82	similar microbiota profiles	56:82	Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.					
33432311	6	77	from	differences	1273:1283	arg1	levels					1301:1306	the relative levels	1288:1306	the relative levels of genera	1288:1316	Differences in proportions of propionate and butyrate end-products were associated with differences in the relative levels of genera.					
33432311	4	78	theme	DNA	910:912	arg1	extraction					914:923	DNA extraction	910:923	DNA extraction	910:923	During fermentation, samples were collected for DNA extraction and 16S rRNA gene amplicon sequencing.					
33432311	4	79	theme	rRNA	933:936	arg1	sequencing					952:961	16S rRNA gene amplicon sequencing	929:961	16S rRNA gene amplicon sequencing	929:961	During fermentation, samples were collected for DNA extraction and 16S rRNA gene amplicon sequencing.					
32977374	2	0	theme	ingested	428:435	arg1	FSP					437:439	ingested FSP	428:439	ingested FSP	428:439	FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact.					
32977374	0	1	theme	Polysaccharides	65:79	arg1	Microbiota					51:60	Human Fecal Microbiota	39:60	Human Fecal Microbiota of Polysaccharides from Flaxseed	39:93	In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed.					
32977374	4	2	theme	Megamonas	819:827	arg1	abundances					754:763	the relative abundances	741:763	the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides	741:862	FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides.					
32977374	1	3	theme	simulated	147:155	arg1	saliva					157:162	simulated saliva	147:162	simulated saliva	147:162	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	3	4	from	Changes	481:487	arg1	content					505:511	carbohydrate content	492:511	carbohydrate content	492:511	Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota.					
32977374	3	4	from	Changes	481:487	arg1	sugars					523:528	reducing sugars	514:528	reducing sugars	514:528	Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota.					
32977374	3	4	from	Changes	481:487	arg1	pH					542:543	culture pH	534:543	culture pH	534:543	Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota.					
32977374	1	5	theme	in	229:230	arg1	fermentation					238:249	in vitro fermentation	229:249	in vitro fermentation of FSP by human gut microbiota	229:280	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	5	6	theme	fatty	936:940	arg1	acids					942:946	short-chain fatty acids	924:946	short-chain fatty acids	924:946	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	5	6	theme	fatty	936:940	arg1	acids					982:986	propionic and butyric acids	960:986	propionic and butyric acids	960:986	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	4	7	dep	composition	631:641	arg1	the					627:629	the	627:629	the	627:629	FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides.					
32977374	2	8	theme	large	457:461	arg1	intestine					463:471	the large intestine intact	453:478	the large intestine intact	453:478	FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact.					
32977374	5	9	theme	acids	942:946	arg1	concentration					907:919	the concentration	903:919	the concentration of short-chain fatty acids, especially propionic and butyric acids	903:986	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	6	10	theme	functional	1043:1052	arg1	food					1054:1057	a functional food	1041:1057	a functional food that benefits gut health	1041:1082	Our results indicate that FSP might be developed as a functional food that benefits gut health.					
32977374	6	10	theme	functional	1043:1052	arg1	FSP					1015:1017	FSP	1015:1017	FSP	1015:1017	Our results indicate that FSP might be developed as a functional food that benefits gut health.					
32977374	0	11	theme	Human	39:43	arg1	Microbiota					51:60	Human Fecal Microbiota	39:60	Human Fecal Microbiota of Polysaccharides from Flaxseed	39:93	In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed.					
32977374	4	12	theme	Phascolarctobacterium	780:800	arg1	abundances					754:763	the relative abundances	741:763	the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides	741:862	FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides.					
32977374	4	13	theme	Firmicutes/Bacteroidetes	695:718	arg1	ratio					720:724	the Firmicutes/Bacteroidetes ratio	691:724	the Firmicutes/Bacteroidetes ratio	691:724	FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides.					
32977374	2	14	theme	reducing	394:401	arg1	sugars					403:408	reducing sugars	394:408	reducing sugars	394:408	FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact.					
32977374	5	15	theme	butyric	974:980	arg1	acids					942:946	short-chain fatty acids	924:946	short-chain fatty acids	924:946	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	5	15	theme	butyric	974:980	arg1	acids					982:986	propionic and butyric acids	960:986	propionic and butyric acids	960:986	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	4	16	theme	gut	664:666	arg1	microbiota					668:677	the gut microbiota	660:677	the gut microbiota	660:677	FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides.					
32977374	0	17	theme	In	0:1	arg1	Digestion					9:17	In Vitro Digestion	0:17	In Vitro Digestion	0:17	In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed.					
32977374	1	18	dep	in	229:230	arg1	vitro					232:236	vitro	232:236	vitro	232:236	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	3	19	theme	reducing	514:521	arg1	sugars					523:528	reducing sugars	514:528	reducing sugars	514:528	Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota.					
32977374	1	20	theme	FSP	254:256	arg1	fermentation					238:249	in vitro fermentation	229:249	in vitro fermentation of FSP by human gut microbiota	229:280	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	3	21	theme	carbohydrate	492:503	arg1	content					505:511	carbohydrate content	492:511	carbohydrate content	492:511	Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota.					
32977374	1	22	theme	small	177:181	arg1	intestine					183:191	small intestine	177:191	small intestine	177:191	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	4	23	theme	microbiota	668:677	arg1	structure					647:655	structure	647:655	structure	647:655	FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides.					
32977374	4	23	theme	microbiota	668:677	arg1	composition					631:641	composition	631:641	composition	631:641	FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides.					
32977374	2	24	theme	molecular	363:371	arg1	weight					373:378	molecular weight	363:378	molecular weight	363:378	FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact.					
32977374	1	25	theme	human	261:265	arg1	microbiota					271:280	human gut microbiota	261:280	human gut microbiota	261:280	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	3	26	theme	culture	534:540	arg1	pH					542:543	culture pH	534:543	culture pH	534:543	Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota.					
32977374	1	27	theme	gut	267:269	arg1	microbiota					271:280	human gut microbiota	261:280	human gut microbiota	261:280	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	2	28	theme	intact	473:478	arg1	intestine					463:471	the large intestine intact	453:478	the large intestine intact	453:478	FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact.					
32977374	5	29	theme	FSP	876:878	arg1	Meanwhile					865:873	Meanwhile	865:873	Meanwhile	865:873	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	5	29	theme	FSP	876:878	arg1	fermentation					880:891	FSP fermentation	876:891	FSP fermentation	876:891	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	0	30	theme	Fecal	45:49	arg1	Microbiota					51:60	Human Fecal Microbiota	39:60	Human Fecal Microbiota of Polysaccharides from Flaxseed	39:93	In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed.					
32977374	3	31	used	used	589:592	arg2	FSP					560:562	FSP	560:562	FSP	560:562	Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota.					
32977374	0	32	from	Microbiota	51:60	arg1	Flaxseed					86:93	Flaxseed	86:93	Flaxseed	86:93	In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed.					
32977374	1	33	dep	saliva	157:162	arg1	conditions					193:202	conditions	193:202	conditions	193:202	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	4	34	theme	Clostridium	803:813	arg1	abundances					754:763	the relative abundances	741:763	the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides	741:862	FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides.					
32977374	2	35	theme	sugars	403:408	arg1	content					383:389	content	383:389	content of reducing sugars	383:408	FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact.					
32977374	2	35	theme	sugars	403:408	arg1	weight					373:378	molecular weight	363:378	molecular weight	363:378	FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact.					
32977374	3	36	theme	gut	597:599	arg1	microbiota					601:610	gut microbiota	597:610	gut microbiota	597:610	Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota.					
32977374	1	37	theme	flaxseed	113:120	arg1	polysaccharides					122:136	flaxseed polysaccharides	113:136	flaxseed polysaccharides (FSP)	113:142	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	1	37	theme	flaxseed	113:120	arg1	FSP					139:141	FSP	139:141	FSP	139:141	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	5	38	theme	propionic	960:968	arg1	acids					942:946	short-chain fatty acids	924:946	short-chain fatty acids	924:946	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	5	38	theme	propionic	960:968	arg1	acids					982:986	propionic and butyric acids	960:986	propionic and butyric acids	960:986	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	1	39	theme	polysaccharides	122:136	arg1	digestion					100:108	The digestion	96:108	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions	96:202	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	1	40	from	digestion	100:108	arg1	gastric					165:171	gastric	165:171	gastric	165:171	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	1	40	from	digestion	100:108	arg1	intestine					183:191	small intestine	177:191	small intestine	177:191	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	1	40	from	digestion	100:108	arg1	saliva					157:162	simulated saliva	147:162	simulated saliva	147:162	The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota.					
32977374	0	41	from	Flaxseed	86:93	arg1	Polysaccharides					65:79	Polysaccharides	65:79	Polysaccharides from Flaxseed	65:93	In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed.					
32977374	0	41	from	Flaxseed	86:93	arg1	Microbiota					51:60	Human Fecal Microbiota	39:60	Human Fecal Microbiota of Polysaccharides from Flaxseed	39:93	In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed.					
32977374	4	42	theme	relative	745:752	arg1	abundances					754:763	the relative abundances	741:763	the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides	741:862	FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides.					
32977374	2	43	from	change	353:358	arg1	content					383:389	content	383:389	content of reducing sugars	383:408	FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact.					
32977374	2	43	from	change	353:358	arg1	weight					373:378	molecular weight	363:378	molecular weight	363:378	FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact.					
32977374	5	44	theme	short-chain	924:934	arg1	acids					942:946	short-chain fatty acids	924:946	short-chain fatty acids	924:946	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	5	44	theme	short-chain	924:934	arg1	acids					982:986	propionic and butyric acids	960:986	propionic and butyric acids	960:986	Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids.					
32977374	6	45	theme	gut	1073:1075	arg1	health					1077:1082	gut health	1073:1082	gut health	1073:1082	Our results indicate that FSP might be developed as a functional food that benefits gut health.					
32977374	0	46	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed.					
32977374	2	47	theme	digestive	321:329	arg1	systems					331:337	the simulated digestive systems	307:337	the simulated digestive systems	307:337	FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact.					
34478797	0	0	theme	ginseng	97:103	arg1	Rubra					105:109	radix ginseng Rubra	91:109	radix ginseng Rubra	91:109	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	11	1	theme	radix	1519:1523	arg1	Rubra					1533:1537	radix ginseng Rubra	1519:1537	radix ginseng Rubra	1519:1537	Our cumulative findings indicate that RGRP-1b obtained from radix ginseng Rubra can function as a strong immune modulator.					
34478797	3	2	theme	RGRP-1b	669:675	arg1	structure					656:664	the structure	652:664	the structure of RGRP-1b	652:675	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	1	3	theme	structure	160:168	arg1	characteristics					170:184	the structure characteristics	156:184	the structure characteristics	156:184	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	0	4	theme	radix	91:95	arg1	Rubra					105:109	radix ginseng Rubra	91:109	radix ginseng Rubra	91:109	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	0	5	from	characteristics	10:24	arg1	Rubra					105:109	radix ginseng Rubra	91:109	radix ginseng Rubra	91:109	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	7	6	theme	O-4	1043:1045	arg1	position					1047:1054	the O-4 position	1039:1054	the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp	1039:1141	Its side chains were branched at the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp.					
34478797	7	7	theme	-β-Galp-	1123:1130	arg1	6 → α-Glcp					1132:1141	-β-Galp-(6 → α-Glcp	1123:1141	1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp	1080:1141	Its side chains were branched at the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp.					
34478797	9	8	theme	IL-6	1285:1288	arg1	expressions					1270:1280	The expressions	1266:1280	The expressions of IL-6, IL-12, and TNF-α	1266:1306	The expressions of IL-6, IL-12, and TNF-α were found to be upregulated after treatment with RGRP-1b.					
34478797	10	9	theme	Huh7	1428:1431	arg1	growth					1418:1423	the growth	1414:1423	the growth of Huh7	1414:1431	RGRP-1b thus possesses the potential to arrest the growth of Huh7 through immunoregulation.					
34478797	4	10	theme	molecular	742:750	arg1	10.2 kDa					774:781	10.2 kDa	774:781	10.2 kDa	774:781	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	4	10	theme	molecular	742:750	arg1	21.3 kDa					761:768	21.3 kDa	761:768	21.3 kDa	761:768	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	4	10	theme	molecular	742:750	arg1	weights					752:758	different molecular weights	732:758	different molecular weights (21.3 kDa and 10.2 kDa, respectively)	732:796	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	1	11	from	characteristics	170:184	arg1	RGR					275:277	RGR	275:277	RGR	275:277	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	11	from	characteristics	170:184	arg1	Rubra					268:272	radix ginseng Rubra	254:272	radix ginseng Rubra (RGR)	254:278	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	6	12	theme	chain	918:922	arg1	structure					924:932	The main chain structure	909:932	The main chain structure of RGRP-1b	909:943	The main chain structure of RGRP-1b was composed of 1,4-α-Glcp, with a 1,4,6-α-Glcp branch unit.					
34478797	7	13	dep	-β-Galp-	1082:1089	arg1	1					1080:1080	1	1080:1080	1	1080:1080	Its side chains were branched at the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp.					
34478797	7	13	dep	-β-Galp-	1082:1089	arg1	-α-Araf-					1097:1104	-α-Araf-	1097:1104	1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp	1080:1141	Its side chains were branched at the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp.					
34478797	7	13	dep	-β-Galp-	1082:1089	arg1	6 → α-Glcp					1132:1141	-β-Galp-(6 → α-Glcp	1123:1141	1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp	1080:1141	Its side chains were branched at the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp.					
34478797	7	13	dep	-β-Galp-	1082:1089	arg1	4 → 1					1091:1095	4 → 1	1091:1095	4 → 1	1091:1095	Its side chains were branched at the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp.					
34478797	6	14	theme	main	913:916	arg1	structure					924:932	The main chain structure	909:932	The main chain structure of RGRP-1b	909:943	The main chain structure of RGRP-1b was composed of 1,4-α-Glcp, with a 1,4,6-α-Glcp branch unit.					
34478797	3	15	theme	emission	457:464	arg1	microscope					484:493	field emission scanning electron microscope	451:493	field emission scanning electron microscope (FESEM)	451:501	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	15	theme	emission	457:464	arg1	FESEM					496:500	FESEM	496:500	FESEM	496:500	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	16	theme	force	511:515	arg1	AFM					529:531	AFM	529:531	AFM	529:531	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	16	theme	force	511:515	arg1	microscope					517:526	atomic force microscope	504:526	atomic force microscope (AFM)	504:532	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	4	17	dep	weights	752:758	arg1	10.2 kDa					774:781	10.2 kDa	774:781	10.2 kDa	774:781	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	4	17	dep	weights	752:758	arg1	21.3 kDa					761:768	21.3 kDa	761:768	21.3 kDa	761:768	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	4	17	dep	weights	752:758	arg1	weights					752:758	different molecular weights	732:758	different molecular weights (21.3 kDa and 10.2 kDa, respectively)	732:796	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	9	18	theme	IL-12	1291:1295	arg1	expressions					1270:1280	The expressions	1266:1280	The expressions of IL-6, IL-12, and TNF-α	1266:1306	The expressions of IL-6, IL-12, and TNF-α were found to be upregulated after treatment with RGRP-1b.					
34478797	11	19	theme	ginseng	1525:1531	arg1	Rubra					1533:1537	radix ginseng Rubra	1519:1537	radix ginseng Rubra	1519:1537	Our cumulative findings indicate that RGRP-1b obtained from radix ginseng Rubra can function as a strong immune modulator.					
34478797	1	20	theme	immune	187:192	arg1	regulation					194:203	immune regulation	187:203	immune regulation	187:203	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	3	21	theme	Monosaccharide	379:392	arg1	composition					394:404	Monosaccharide composition	379:404	Monosaccharide composition	379:404	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	0	22	from	activities	47:56	arg1	Rubra					105:109	radix ginseng Rubra	91:109	radix ginseng Rubra	91:109	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	3	23	theme	Fourier-transformed	535:553	arg1	FT-IR					575:579	FT-IR	575:579	FT-IR	575:579	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	23	theme	Fourier-transformed	535:553	arg1	resonance					564:572	Fourier-transformed infrared resonance	535:572	Fourier-transformed infrared resonance (FT-IR)	535:580	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	24	theme	GC-MS	424:428	arg1	analyses					430:437	GC-MS analyses	424:437	GC-MS analyses	424:437	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	0	25	theme	Structure	0:8	arg1	characteristics					10:24	Structure characteristics	0:24	Structure characteristics	0:24	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	7	26	theme	side	1010:1013	arg1	chains					1015:1020	Its side chains	1006:1020	Its side chains	1006:1020	Its side chains were branched at the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp.					
34478797	3	27	theme	infrared	555:562	arg1	FT-IR					575:579	FT-IR	575:579	FT-IR	575:579	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	27	theme	infrared	555:562	arg1	resonance					564:572	Fourier-transformed infrared resonance	535:572	Fourier-transformed infrared resonance (FT-IR)	535:580	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	6	28	theme	1,4,6-α-Glcp	980:991	arg1	unit					1000:1003	a 1,4,6-α-Glcp branch unit	978:1003	a 1,4,6-α-Glcp branch unit	978:1003	The main chain structure of RGRP-1b was composed of 1,4-α-Glcp, with a 1,4,6-α-Glcp branch unit.					
34478797	0	29	theme	immunomodulatory	30:45	arg1	activities					47:56	immunomodulatory activities	30:56	immunomodulatory activities	30:56	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	8	30	theme	oxide	1170:1174	arg1	levels					1181:1186	the nitric oxide (NO) levels	1159:1186	the nitric oxide (NO) levels	1159:1186	The changes in the nitric oxide (NO) levels and cytotoxicity revealed that macrophages probably get activated by RGRP-1b.					
34478797	2	31	theme	RGR	299:301	arg1	RGRP					320:323	RGRP	320:323	RGRP	320:323	For this purpose, RGR polysaccharides (RGRP) were purified through DEAE and S-300 chromatography.					
34478797	2	31	theme	RGR	299:301	arg1	polysaccharides					303:317	RGR polysaccharides	299:317	RGR polysaccharides (RGRP)	299:324	For this purpose, RGR polysaccharides (RGRP) were purified through DEAE and S-300 chromatography.					
34478797	3	32	theme	field	451:455	arg1	microscope					484:493	field emission scanning electron microscope	451:493	field emission scanning electron microscope (FESEM)	451:501	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	32	theme	field	451:455	arg1	FESEM					496:500	FESEM	496:500	FESEM	496:500	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	4	33	theme	different	732:740	arg1	10.2 kDa					774:781	10.2 kDa	774:781	10.2 kDa	774:781	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	4	33	theme	different	732:740	arg1	21.3 kDa					761:768	21.3 kDa	761:768	21.3 kDa	761:768	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	4	33	theme	different	732:740	arg1	weights					752:758	different molecular weights	732:758	different molecular weights (21.3 kDa and 10.2 kDa, respectively)	732:796	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	11	34	theme	strong	1557:1562	arg1	RGRP-1b					1497:1503	RGRP-1b	1497:1503	RGRP-1b obtained from radix ginseng Rubra	1497:1537	Our cumulative findings indicate that RGRP-1b obtained from radix ginseng Rubra can function as a strong immune modulator.					
34478797	11	34	theme	strong	1557:1562	arg1	modulator					1571:1579	a strong immune modulator	1555:1579	a strong immune modulator	1555:1579	Our cumulative findings indicate that RGRP-1b obtained from radix ginseng Rubra can function as a strong immune modulator.					
34478797	1	35	theme	present	116:122	arg1	study					124:128	The present study	112:128	The present study	112:128	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	36	theme	anti-cancer	210:220	arg1	abilities					222:230	anti-cancer abilities	210:230	anti-cancer abilities	210:230	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	7	37	theme	1,4,6-α-Glcp	1059:1070	arg1	position					1047:1054	the O-4 position	1039:1054	the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp	1039:1141	Its side chains were branched at the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp.					
34478797	1	38	from	Rubra	268:272	arg1	regulation					194:203	immune regulation	187:203	immune regulation	187:203	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	38	from	Rubra	268:272	arg1	abilities					222:230	anti-cancer abilities	210:230	anti-cancer abilities	210:230	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	38	from	Rubra	268:272	arg1	characteristics					170:184	the structure characteristics	156:184	the structure characteristics	156:184	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	11	39	theme	immune	1564:1569	arg1	RGRP-1b					1497:1503	RGRP-1b	1497:1503	RGRP-1b obtained from radix ginseng Rubra	1497:1537	Our cumulative findings indicate that RGRP-1b obtained from radix ginseng Rubra can function as a strong immune modulator.					
34478797	11	39	theme	immune	1564:1569	arg1	modulator					1571:1579	a strong immune modulator	1555:1579	a strong immune modulator	1555:1579	Our cumulative findings indicate that RGRP-1b obtained from radix ginseng Rubra can function as a strong immune modulator.					
34478797	4	40	contain	possess	724:730	arg1	RGRP-1b					716:722	RGRP-1b	716:722	RGRP-1b	716:722	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	4	40	contain	possess	724:730	arg2	10.2 kDa					774:781	10.2 kDa	774:781	10.2 kDa	774:781	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	4	40	contain	possess	724:730	arg1	RGRP-1a					704:710	RGRP-1a	704:710	RGRP-1a	704:710	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	4	40	contain	possess	724:730	arg2	21.3 kDa					761:768	21.3 kDa	761:768	21.3 kDa	761:768	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	4	40	contain	possess	724:730	arg2	weights					752:758	different molecular weights	732:758	different molecular weights (21.3 kDa and 10.2 kDa, respectively)	732:796	Our results revealed that RGRP-1a and RGRP-1b possess different molecular weights (21.3 kDa and 10.2 kDa, respectively).					
34478797	3	41	theme	resonance	604:612	arg1	spectra					620:626	nuclear magnetic resonance (NMR) spectra	587:626	nuclear magnetic resonance (NMR) spectra	587:626	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	1	42	from	abilities	222:230	arg1	RGR					275:277	RGR	275:277	RGR	275:277	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	42	from	abilities	222:230	arg1	Rubra					268:272	radix ginseng Rubra	254:272	radix ginseng Rubra (RGR)	254:278	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	6	43	theme	branch	993:998	arg1	unit					1000:1003	a 1,4,6-α-Glcp branch unit	978:1003	a 1,4,6-α-Glcp branch unit	978:1003	The main chain structure of RGRP-1b was composed of 1,4-α-Glcp, with a 1,4,6-α-Glcp branch unit.					
34478797	0	44	theme	polysaccharide	63:76	arg1	RGRP-1b					78:84	a polysaccharide RGRP-1b	61:84	a polysaccharide RGRP-1b from radix ginseng Rubra	61:109	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	8	45	theme	nitric	1163:1168	arg1	oxide					1170:1174	nitric oxide	1163:1174	the nitric oxide (NO) levels	1159:1186	The changes in the nitric oxide (NO) levels and cytotoxicity revealed that macrophages probably get activated by RGRP-1b.					
34478797	8	45	theme	nitric	1163:1168	arg1	NO					1177:1178	NO	1177:1178	NO	1177:1178	The changes in the nitric oxide (NO) levels and cytotoxicity revealed that macrophages probably get activated by RGRP-1b.					
34478797	3	46	used	used	634:637	arg2	spectra					620:626	nuclear magnetic resonance (NMR) spectra	587:626	nuclear magnetic resonance (NMR) spectra	587:626	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	46	used	used	634:637	arg2	microscope					517:526	atomic force microscope	504:526	atomic force microscope (AFM)	504:532	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	46	used	used	634:637	arg2	microscope					484:493	field emission scanning electron microscope	451:493	field emission scanning electron microscope (FESEM)	451:501	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	46	used	used	634:637	arg2	AFM					529:531	AFM	529:531	AFM	529:531	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	46	used	used	634:637	arg2	FESEM					496:500	FESEM	496:500	FESEM	496:500	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	46	used	used	634:637	arg2	resonance					564:572	Fourier-transformed infrared resonance	535:572	Fourier-transformed infrared resonance (FT-IR)	535:580	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	46	used	used	634:637	arg2	composition					394:404	Monosaccharide composition	379:404	Monosaccharide composition	379:404	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	46	used	used	634:637	arg2	analyses					430:437	GC-MS analyses	424:437	GC-MS analyses	424:437	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	46	used	used	634:637	arg2	FT-IR					575:579	FT-IR	575:579	FT-IR	575:579	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	46	used	used	634:637	arg2	methylation					407:417	methylation	407:417	methylation	407:417	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	9	47	with	treatment	1343:1351	arg1	RGRP-1b					1358:1364	RGRP-1b	1358:1364	RGRP-1b	1358:1364	The expressions of IL-6, IL-12, and TNF-α were found to be upregulated after treatment with RGRP-1b.					
34478797	1	48	from	polysaccharides	235:249	arg1	RGR					275:277	RGR	275:277	RGR	275:277	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	48	from	polysaccharides	235:249	arg1	Rubra					268:272	radix ginseng Rubra	254:272	radix ginseng Rubra (RGR)	254:278	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	3	49	theme	nuclear	587:593	arg1	NMR					615:617	NMR	615:617	NMR	615:617	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	49	theme	nuclear	587:593	arg1	resonance					604:612	nuclear magnetic resonance	587:612	nuclear magnetic resonance (NMR) spectra	587:626	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	50	theme	scanning	466:473	arg1	microscope					484:493	field emission scanning electron microscope	451:493	field emission scanning electron microscope (FESEM)	451:501	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	50	theme	scanning	466:473	arg1	FESEM					496:500	FESEM	496:500	FESEM	496:500	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	1	51	theme	polysaccharides	235:249	arg1	regulation					194:203	immune regulation	187:203	immune regulation	187:203	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	51	theme	polysaccharides	235:249	arg1	abilities					222:230	anti-cancer abilities	210:230	anti-cancer abilities	210:230	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	51	theme	polysaccharides	235:249	arg1	characteristics					170:184	the structure characteristics	156:184	the structure characteristics	156:184	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	7	52	dep	-α-Araf-	1097:1104	arg1	5 → α-Araf					1106:1115	5 → α-Araf	1106:1115	5 → α-Araf	1106:1115	Its side chains were branched at the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp.					
34478797	7	52	dep	-α-Araf-	1097:1104	arg1	1					1121:1121	1	1121:1121	1	1121:1121	Its side chains were branched at the O-4 position of 1,4,6-α-Glcp, namely 1)-β-Galp-(4 → 1)-α-Araf-(5 → α-Araf and 1)-β-Galp-(6 → α-Glcp.					
34478797	3	53	theme	magnetic	595:602	arg1	NMR					615:617	NMR	615:617	NMR	615:617	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	53	theme	magnetic	595:602	arg1	resonance					604:612	nuclear magnetic resonance	587:612	nuclear magnetic resonance (NMR) spectra	587:626	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	1	54	from	regulation	194:203	arg1	RGR					275:277	RGR	275:277	RGR	275:277	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	54	from	regulation	194:203	arg1	Rubra					268:272	radix ginseng Rubra	254:272	radix ginseng Rubra (RGR)	254:278	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	3	55	theme	electron	475:482	arg1	microscope					484:493	field emission scanning electron microscope	451:493	field emission scanning electron microscope (FESEM)	451:501	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	55	theme	electron	475:482	arg1	FESEM					496:500	FESEM	496:500	FESEM	496:500	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	0	56	from	Rubra	105:109	arg1	RGRP-1b					78:84	a polysaccharide RGRP-1b	61:84	a polysaccharide RGRP-1b from radix ginseng Rubra	61:109	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	0	56	from	Rubra	105:109	arg1	characteristics					10:24	Structure characteristics	0:24	Structure characteristics	0:24	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	0	56	from	Rubra	105:109	arg1	activities					47:56	immunomodulatory activities	30:56	immunomodulatory activities	30:56	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	10	57	contain	possesses	1380:1388	arg1	RGRP-1b					1367:1373	RGRP-1b	1367:1373	RGRP-1b	1367:1373	RGRP-1b thus possesses the potential to arrest the growth of Huh7 through immunoregulation.					
34478797	10	57	contain	possesses	1380:1388	arg2	potential					1394:1402	the potential to arrest the growth of Huh7 through immunoregulation	1390:1456	the potential to arrest the growth of Huh7 through immunoregulation	1390:1456	RGRP-1b thus possesses the potential to arrest the growth of Huh7 through immunoregulation.					
34478797	8	58	from	changes	1148:1154	arg1	cytotoxicity					1192:1203	cytotoxicity	1192:1203	cytotoxicity	1192:1203	The changes in the nitric oxide (NO) levels and cytotoxicity revealed that macrophages probably get activated by RGRP-1b.					
34478797	8	58	from	changes	1148:1154	arg1	levels					1181:1186	the nitric oxide (NO) levels	1159:1186	the nitric oxide (NO) levels	1159:1186	The changes in the nitric oxide (NO) levels and cytotoxicity revealed that macrophages probably get activated by RGRP-1b.					
34478797	2	59	theme	S-300	357:361	arg1	chromatography					363:376	S-300 chromatography	357:376	S-300 chromatography	357:376	For this purpose, RGR polysaccharides (RGRP) were purified through DEAE and S-300 chromatography.					
34478797	1	60	theme	radix	254:258	arg1	RGR					275:277	RGR	275:277	RGR	275:277	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	60	theme	radix	254:258	arg1	Rubra					268:272	radix ginseng Rubra	254:272	radix ginseng Rubra (RGR)	254:278	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	0	61	theme	RGRP-1b	78:84	arg1	characteristics					10:24	Structure characteristics	0:24	Structure characteristics	0:24	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	0	61	theme	RGRP-1b	78:84	arg1	activities					47:56	immunomodulatory activities	30:56	immunomodulatory activities	30:56	Structure characteristics and immunomodulatory activities of a polysaccharide RGRP-1b from radix ginseng Rubra.					
34478797	9	62	theme	TNF-α	1302:1306	arg1	expressions					1270:1280	The expressions	1266:1280	The expressions of IL-6, IL-12, and TNF-α	1266:1306	The expressions of IL-6, IL-12, and TNF-α were found to be upregulated after treatment with RGRP-1b.					
34478797	6	63	theme	RGRP-1b	937:943	arg1	structure					924:932	The main chain structure	909:932	The main chain structure of RGRP-1b	909:943	The main chain structure of RGRP-1b was composed of 1,4-α-Glcp, with a 1,4,6-α-Glcp branch unit.					
34478797	11	64	theme	cumulative	1463:1472	arg1	findings					1474:1481	Our cumulative findings	1459:1481	Our cumulative findings	1459:1481	Our cumulative findings indicate that RGRP-1b obtained from radix ginseng Rubra can function as a strong immune modulator.					
34478797	3	65	theme	atomic	504:509	arg1	AFM					529:531	AFM	529:531	AFM	529:531	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	3	65	theme	atomic	504:509	arg1	microscope					517:526	atomic force microscope	504:526	atomic force microscope (AFM)	504:532	Monosaccharide composition, methylation, and GC-MS analyses, as well as field emission scanning electron microscope (FESEM), atomic force microscope (AFM), Fourier-transformed infrared resonance (FT-IR), and nuclear magnetic resonance (NMR) spectra, were used to establish the structure of RGRP-1b.					
34478797	1	66	theme	ginseng	260:266	arg1	RGR					275:277	RGR	275:277	RGR	275:277	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
34478797	1	66	theme	ginseng	260:266	arg1	Rubra					268:272	radix ginseng Rubra	254:272	radix ginseng Rubra (RGR)	254:278	The present study was undertaken to explore the structure characteristics, immune regulation, and anti-cancer abilities of polysaccharides in radix ginseng Rubra (RGR).					
33436169	6	0	theme	distinguishing	953:966	arg1	ratio					978:982	a distinguishing 1:1 molar ratio	951:982	a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3)	951:1238	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	1	theme	side	1021:1024	arg1	chains					1026:1031	side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3)	1021:1238	a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3)	951:1238	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	9	2	theme	potential	1665:1673	arg1	applications					1675:1686	future potential applications	1658:1686	future potential applications of AELP in the food and medical industries	1658:1729	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	8	3	theme	binding	1457:1463	arg1	affinity					1465:1472	the binding affinity	1453:1472	the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M)	1453:1514	Surface plasmon resonance showed the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M) was about 10 times stronger than that of AELP-B5 (4.588 × 10-4 M).					
33436169	1	4	theme	Mw	241:242	arg1	B6					237:238	B6	237:238	B6 (Mw, 1.56 × 104 g/mol)	237:261	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	4	theme	Mw	241:242	arg1	g/mol					256:260	Mw, 1.56 × 104 g/mol	241:260	g/mol	256:260	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	8	5	theme	10	1526:1527	arg1	times					1529:1533	times	1529:1533	times	1529:1533	Surface plasmon resonance showed the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M) was about 10 times stronger than that of AELP-B5 (4.588 × 10-4 M).					
33436169	9	6	theme	food	1703:1706	arg1	industries					1720:1729	the food and medical industries	1699:1729	the food and medical industries	1699:1729	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	0	7	theme	Aralia	124:129	arg1	elata					131:135	Aralia elata	124:135	Aralia elata	124:135	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	1	8	theme	Mw	211:212	arg1	g/mol					226:230	Mw, 4.25 × 104 g/mol	211:230	g/mol	226:230	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	8	theme	Mw	211:212	arg1	AELP-B5					202:208	AELP-B5	202:208	AELP-B5 (Mw, 4.25 × 104 g/mol)	202:231	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	6	9	theme	repeating	835:843	arg1	moiety					858:863	a repeating disaccharide moiety	833:863	a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio	833:928	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	9	10	theme	medical	1712:1718	arg1	industries					1720:1729	the food and medical industries	1699:1729	the food and medical industries	1699:1729	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	6	11	contain	possessed	787:795	arg2	moiety					858:863	a repeating disaccharide moiety	833:863	a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio	833:928	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	11	contain	possessed	787:795	arg2	→4GalA1→					797:804	→4GalA1→	797:804	→4GalA1→ as smooth regions (HG)	797:827	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	11	contain	possessed	787:795	arg1	AELP-B5					779:785	(1) AELP-B5	775:785	(1) AELP-B5	775:785	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	7	12	contain	possessed	1344:1352	arg1	AELP-B6					1336:1342	AELP-B6	1336:1342	AELP-B6	1336:1342	The CCK-8 assay illustrated that AELP-B6 possessed higher cytotoxicity against HepG2 and HT-29 than that of AELP-B5.					
33436169	7	12	contain	possessed	1344:1352	arg2	cytotoxicity					1361:1372	higher cytotoxicity	1354:1372	higher cytotoxicity	1354:1372	The CCK-8 assay illustrated that AELP-B6 possessed higher cytotoxicity against HepG2 and HT-29 than that of AELP-B5.					
33436169	0	13	from	leaves	114:119	arg1	structures					27:36	structures	27:36	structures	27:36	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	0	13	from	leaves	114:119	arg1	potentials					53:62	anti-tumor potentials	42:62	anti-tumor potentials	42:62	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	0	13	from	leaves	114:119	arg1	isolation					16:24	Ultrafiltration isolation	0:24	Ultrafiltration isolation	0:24	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	0	13	from	leaves	114:119	arg1	pectins					101:107	two arabinose- and galactose-rich pectins	67:107	two arabinose- and galactose-rich pectins from leaves of Aralia elata	67:135	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	9	14	from	AELP	1691:1694	arg1	industries					1720:1729	the food and medical industries	1699:1729	the food and medical industries	1699:1729	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	5	15	theme	Smith	651:655	arg1	degradation					657:667	Smith degradation	651:667	Smith degradation	651:667	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	9	16	from	applications	1675:1686	arg1	industries					1720:1729	the food and medical industries	1699:1729	the food and medical industries	1699:1729	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	1	17	theme	arabinose-	148:157	arg1	B6					237:238	B6	237:238	B6 (Mw, 1.56 × 104 g/mol)	237:261	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	17	theme	arabinose-	148:157	arg1	AELP-B5					202:208	AELP-B5	202:208	AELP-B5 (Mw, 4.25 × 104 g/mol)	202:231	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	17	theme	arabinose-	148:157	arg1	polysaccharides					185:199	arabinose- and galactose-rich pectic polysaccharides	148:199	Two novel arabinose- and galactose-rich pectic polysaccharides	138:199	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	8	18	dep	showed	1446:1451	arg1	times					1529:1533	times	1529:1533	times	1529:1533	Surface plasmon resonance showed the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M) was about 10 times stronger than that of AELP-B5 (4.588 × 10-4 M).					
33436169	5	19	theme	planar	539:544	arg1	structures					546:555	The planar structures	535:555	The planar structures	535:555	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	1	20	theme	galactose-rich	163:176	arg1	B6					237:238	B6	237:238	B6 (Mw, 1.56 × 104 g/mol)	237:261	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	20	theme	galactose-rich	163:176	arg1	AELP-B5					202:208	AELP-B5	202:208	AELP-B5 (Mw, 4.25 × 104 g/mol)	202:231	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	20	theme	galactose-rich	163:176	arg1	polysaccharides					185:199	arabinose- and galactose-rich pectic polysaccharides	148:199	Two novel arabinose- and galactose-rich pectic polysaccharides	138:199	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	6	21	theme	hairy	886:890	arg1	RG-I					901:904	RG-I	901:904	RG-I	901:904	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	21	theme	hairy	886:890	arg1	regions					892:898	hairy regions	886:898	hairy regions (RG-I) with a 1:5 molar ratio	886:928	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	4	22	theme	AELP-B5	443:449	arg1	backbone					411:418	The structural backbone and branched chains	396:438	backbone	411:418	The structural backbone and branched chains of AELP-B5 and B6 were preliminarily elucidated by mild acid hydrolysis with HILIC-ESI--MS/MS.					
33436169	4	22	theme	AELP-B5	443:449	arg1	chains					433:438	The structural backbone and branched chains	396:438	chains	433:438	The structural backbone and branched chains of AELP-B5 and B6 were preliminarily elucidated by mild acid hydrolysis with HILIC-ESI--MS/MS.					
33436169	8	23	dep	times	1529:1533	arg1	stronger					1535:1542	stronger	1535:1542	stronger	1535:1542	Surface plasmon resonance showed the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M) was about 10 times stronger than that of AELP-B5 (4.588 × 10-4 M).					
33436169	0	24	theme	pectins	101:107	arg1	structures					27:36	structures	27:36	structures	27:36	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	0	24	theme	pectins	101:107	arg1	potentials					53:62	anti-tumor potentials	42:62	anti-tumor potentials	42:62	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	0	24	theme	pectins	101:107	arg1	isolation					16:24	Ultrafiltration isolation	0:24	Ultrafiltration isolation	0:24	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	6	25	theme	→4GalA1→2Rha1→	868:881	arg1	→4GalA1→					797:804	→4GalA1→	797:804	→4GalA1→ as smooth regions (HG)	797:827	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	25	theme	→4GalA1→2Rha1→	868:881	arg1	moiety					858:863	a repeating disaccharide moiety	833:863	a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio	833:928	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	0	26	from	potentials	53:62	arg1	leaves					114:119	leaves	114:119	leaves	114:119	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	1	27	theme	Aralia	305:310	arg1	elata					312:316	Aralia elata	305:316	Aralia elata	305:316	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	4	28	theme	acid	496:499	arg1	hydrolysis					501:510	mild acid hydrolysis	491:510	mild acid hydrolysis with HILIC-ESI--MS/MS	491:532	The structural backbone and branched chains of AELP-B5 and B6 were preliminarily elucidated by mild acid hydrolysis with HILIC-ESI--MS/MS.					
33436169	0	29	theme	arabinose-	71:80	arg1	pectins					101:107	two arabinose- and galactose-rich pectins	67:107	two arabinose- and galactose-rich pectins from leaves of Aralia elata	67:135	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	0	30	theme	Ultrafiltration	0:14	arg1	structures					27:36	structures	27:36	structures	27:36	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	0	30	theme	Ultrafiltration	0:14	arg1	potentials					53:62	anti-tumor potentials	42:62	anti-tumor potentials	42:62	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	0	30	theme	Ultrafiltration	0:14	arg1	isolation					16:24	Ultrafiltration isolation	0:24	Ultrafiltration isolation	0:24	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	1	31	dep	the	291:293	arg1	leaves					295:300	leaves	295:300	leaves	295:300	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	6	32	dep	had	947:949	arg1	both					1240:1243	both	1240:1243	both	1240:1243	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	1	33	theme	×	250:250	arg1	B6					237:238	B6	237:238	B6 (Mw, 1.56 × 104 g/mol)	237:261	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	33	theme	×	250:250	arg1	g/mol					256:260	Mw, 1.56 × 104 g/mol	241:260	g/mol	256:260	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	6	34	theme	molar	918:922	arg1	ratio					924:928	a 1:5 molar ratio	912:928	a 1:5 molar ratio	912:928	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	9	35	theme	above	1587:1591	arg1	findings					1593:1600	The above findings	1583:1600	The above findings	1583:1600	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	1	36	dep	obtained	277:284	arg1	Miq					319:321	Miq	319:321	Miq	319:321	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	6	37	theme	1:5	914:916	arg1	ratio					924:928	a 1:5 molar ratio	912:928	a 1:5 molar ratio	912:928	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	3	38	theme	membrane	378:385	arg1	columns					387:393	ultrafiltration membrane columns	362:393	ultrafiltration membrane columns	362:393	with anion resin and sequenced ultrafiltration membrane columns.					
33436169	6	39	with	regions	892:898	arg1	ratio					924:928	a 1:5 molar ratio	912:928	a 1:5 molar ratio	912:928	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	4	40	theme	B6	455:456	arg1	backbone					411:418	The structural backbone and branched chains	396:438	backbone	411:418	The structural backbone and branched chains of AELP-B5 and B6 were preliminarily elucidated by mild acid hydrolysis with HILIC-ESI--MS/MS.					
33436169	4	40	theme	B6	455:456	arg1	chains					433:438	The structural backbone and branched chains	396:438	chains	433:438	The structural backbone and branched chains of AELP-B5 and B6 were preliminarily elucidated by mild acid hydrolysis with HILIC-ESI--MS/MS.					
33436169	9	41	theme	molecular	1612:1620	arg1	structure					1622:1630	a molecular structure	1610:1630	a molecular structure	1610:1630	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	1	42	theme	×	220:220	arg1	g/mol					226:230	Mw, 4.25 × 104 g/mol	211:230	g/mol	226:230	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	42	theme	×	220:220	arg1	AELP-B5					202:208	AELP-B5	202:208	AELP-B5 (Mw, 4.25 × 104 g/mol)	202:231	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	8	43	theme	AELP-B6	1477:1483	arg1	affinity					1465:1472	the binding affinity	1453:1472	the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M)	1453:1514	Surface plasmon resonance showed the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M) was about 10 times stronger than that of AELP-B5 (4.588 × 10-4 M).					
33436169	6	44	theme	1:1	968:970	arg1	ratio					978:982	a distinguishing 1:1 molar ratio	951:982	a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3)	951:1238	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	8	45	theme	Surface	1420:1426	arg1	resonance					1436:1444	Surface plasmon resonance	1420:1444	Surface plasmon resonance	1420:1444	Surface plasmon resonance showed the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M) was about 10 times stronger than that of AELP-B5 (4.588 × 10-4 M).					
33436169	6	46	theme	branched	1262:1269	arg1	conformations					1288:1300	highly branched and compact coil conformations	1255:1300	highly branched and compact coil conformations	1255:1300	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	47	theme	smooth	809:814	arg1	HG					825:826	HG	825:826	HG	825:826	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	47	theme	smooth	809:814	arg1	regions					816:822	smooth regions	809:822	smooth regions (HG)	809:827	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	9	48	theme	future	1658:1663	arg1	applications					1675:1686	future potential applications	1658:1686	future potential applications of AELP in the food and medical industries	1658:1729	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	4	49	with	hydrolysis	501:510	arg1	HILIC-ESI--MS/MS					517:532	HILIC-ESI--MS/MS	517:532	HILIC-ESI--MS/MS	517:532	The structural backbone and branched chains of AELP-B5 and B6 were preliminarily elucidated by mild acid hydrolysis with HILIC-ESI--MS/MS.					
33436169	6	50	theme	complex	1013:1019	arg1	chains					1026:1031	side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3)	1021:1238	a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3)	951:1238	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	1	51	theme	pectic	178:183	arg1	B6					237:238	B6	237:238	B6 (Mw, 1.56 × 104 g/mol)	237:261	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	51	theme	pectic	178:183	arg1	AELP-B5					202:208	AELP-B5	202:208	AELP-B5 (Mw, 4.25 × 104 g/mol)	202:231	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	51	theme	pectic	178:183	arg1	polysaccharides					185:199	arabinose- and galactose-rich pectic polysaccharides	148:199	Two novel arabinose- and galactose-rich pectic polysaccharides	138:199	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	9	52	theme	AELP	1691:1694	arg1	applications					1675:1686	future potential applications	1658:1686	future potential applications of AELP in the food and medical industries	1658:1729	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	6	53	theme	disaccharide	845:856	arg1	moiety					858:863	a repeating disaccharide moiety	833:863	a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio	833:928	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	5	54	theme	methylation	673:683	arg1	analysis					685:692	methylation analysis	673:692	methylation analysis	673:692	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	6	55	contain	had	947:949	arg1	AELP-B6					939:945	AELP-B6	939:945	AELP-B6	939:945	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	55	contain	had	947:949	arg2	ratio					978:982	a distinguishing 1:1 molar ratio	951:982	a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3)	951:1238	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	5	56	dep	NMR	702:704	arg1	HMBC					727:730	HMBC	727:730	HMBC	727:730	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	5	56	dep	NMR	702:704	arg1	TOCSY					749:753	TOCSY	749:753	TOCSY	749:753	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	5	56	dep	NMR	702:704	arg1	1H/13C					707:712	1H/13C	707:712	1H/13C	707:712	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	5	56	dep	NMR	702:704	arg1	DEPT					715:718	DEPT	715:718	DEPT	715:718	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	5	56	dep	NMR	702:704	arg1	COSY					733:736	COSY	733:736	COSY	733:736	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	5	56	dep	NMR	702:704	arg1	NOESY					739:743	NOESY	739:743	NOESY	739:743	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	5	56	dep	NMR	702:704	arg1	HSQC					721:724	HSQC	721:724	HSQC	721:724	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	6	57	dep	chains	1026:1031	arg1	constituted					1038:1048	constituted	1038:1048	were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6	1033:1229	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	57	dep	chains	1026:1031	arg1	3					1237:1237	3	1237:1237	3	1237:1237	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	57	dep	chains	1026:1031	arg1	2					1010:1010	2	1010:1010	2	1010:1010	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	58	theme	AELP-B5	1216:1222	arg1	RG-I					1208:1211	RG-I	1208:1211	RG-I of AELP-B5 and B6	1208:1229	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	1	59	theme	novel	142:146	arg1	B6					237:238	B6	237:238	B6 (Mw, 1.56 × 104 g/mol)	237:261	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	59	theme	novel	142:146	arg1	AELP-B5					202:208	AELP-B5	202:208	AELP-B5 (Mw, 4.25 × 104 g/mol)	202:231	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	59	theme	novel	142:146	arg1	polysaccharides					185:199	arabinose- and galactose-rich pectic polysaccharides	148:199	Two novel arabinose- and galactose-rich pectic polysaccharides	138:199	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	0	60	from	structures	27:36	arg1	leaves					114:119	leaves	114:119	leaves	114:119	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	6	61	theme	rhamnosyl	1189:1197	arg1	units					1199:1203	the rhamnosyl units	1185:1203	the rhamnosyl units in RG-I of AELP-B5 and B6	1185:1229	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	0	62	theme	galactose-rich	86:99	arg1	pectins					101:107	two arabinose- and galactose-rich pectins	67:107	two arabinose- and galactose-rich pectins from leaves of Aralia elata	67:135	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	8	63	theme	×	1572:1572	arg1	M					1579:1579	4.588 × 10-4 M	1566:1579	4.588 × 10-4 M	1566:1579	Surface plasmon resonance showed the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M) was about 10 times stronger than that of AELP-B5 (4.588 × 10-4 M).					
33436169	8	63	theme	×	1572:1572	arg1	AELP-B5					1557:1563	AELP-B5	1557:1563	AELP-B5 (4.588 × 10-4 M)	1557:1580	Surface plasmon resonance showed the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M) was about 10 times stronger than that of AELP-B5 (4.588 × 10-4 M).					
33436169	5	64	theme	spatial	561:567	arg1	configurations					569:582	spatial configurations	561:582	spatial configurations	561:582	The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for compositions, Smith degradation and methylation analysis, FT-IR, NMR (1H/13C, DEPT, HSQC, HMBC, COSY, NOESY and TOCSY) and SEC-MALLS-RID.					
33436169	4	65	theme	branched	424:431	arg1	chains					433:438	The structural backbone and branched chains	396:438	chains	433:438	The structural backbone and branched chains of AELP-B5 and B6 were preliminarily elucidated by mild acid hydrolysis with HILIC-ESI--MS/MS.					
33436169	3	66	theme	ultrafiltration	362:376	arg1	columns					387:393	ultrafiltration membrane columns	362:393	ultrafiltration membrane columns	362:393	with anion resin and sequenced ultrafiltration membrane columns.					
33436169	4	67	theme	structural	400:409	arg1	backbone					411:418	The structural backbone and branched chains	396:438	backbone	411:418	The structural backbone and branched chains of AELP-B5 and B6 were preliminarily elucidated by mild acid hydrolysis with HILIC-ESI--MS/MS.					
33436169	4	68	theme	mild	491:494	arg1	hydrolysis					501:510	mild acid hydrolysis	491:510	mild acid hydrolysis with HILIC-ESI--MS/MS	491:532	The structural backbone and branched chains of AELP-B5 and B6 were preliminarily elucidated by mild acid hydrolysis with HILIC-ESI--MS/MS.					
33436169	0	69	theme	anti-tumor	42:51	arg1	potentials					53:62	anti-tumor potentials	42:62	anti-tumor potentials	42:62	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	0	69	theme	anti-tumor	42:51	arg1	isolation					16:24	Ultrafiltration isolation	0:24	Ultrafiltration isolation	0:24	Ultrafiltration isolation, structures and anti-tumor potentials of two arabinose- and galactose-rich pectins from leaves of Aralia elata.					
33436169	1	70	theme	104	252:254	arg1	B6					237:238	B6	237:238	B6 (Mw, 1.56 × 104 g/mol)	237:261	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	70	theme	104	252:254	arg1	g/mol					256:260	Mw, 1.56 × 104 g/mol	241:260	g/mol	256:260	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	7	71	theme	higher	1354:1359	arg1	cytotoxicity					1361:1372	higher cytotoxicity	1354:1372	higher cytotoxicity	1354:1372	The CCK-8 assay illustrated that AELP-B6 possessed higher cytotoxicity against HepG2 and HT-29 than that of AELP-B5.					
33436169	6	72	theme	compact	1275:1281	arg1	conformations					1288:1300	highly branched and compact coil conformations	1255:1300	highly branched and compact coil conformations	1255:1300	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	9	73	from	industries	1720:1729	arg1	applications					1675:1686	future potential applications	1658:1686	future potential applications of AELP in the food and medical industries	1658:1729	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	3	74	theme	anion	336:340	arg1	resin					342:346	anion resin	336:346	anion resin	336:346	with anion resin and sequenced ultrafiltration membrane columns.					
33436169	6	75	theme	B6	1228:1229	arg1	RG-I					1208:1211	RG-I	1208:1211	RG-I of AELP-B5 and B6	1208:1229	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	76	from	units	1199:1203	arg1	RG-I					1208:1211	RG-I	1208:1211	RG-I of AELP-B5 and B6	1208:1229	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	9	77	theme	structure	1622:1630	arg1	basis					1648:1652	a molecular structure and bioactivity basis	1610:1652	a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries	1610:1729	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	6	78	dep	AELP-B5	779:785	arg1	1					776:776	1	776:776	1	776:776	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	79	dep	ratio	978:982	arg1	chains					1026:1031	side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3)	1021:1238	a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3)	951:1238	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	6	80	theme	coil	1283:1286	arg1	conformations					1288:1300	highly branched and compact coil conformations	1255:1300	highly branched and compact coil conformations	1255:1300	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	1	81	theme	104	222:224	arg1	g/mol					226:230	Mw, 4.25 × 104 g/mol	211:230	g/mol	226:230	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	1	81	theme	104	222:224	arg1	AELP-B5					202:208	AELP-B5	202:208	AELP-B5 (Mw, 4.25 × 104 g/mol)	202:231	Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.)					
33436169	7	82	theme	CCK-8	1307:1311	arg1	assay					1313:1317	The CCK-8 assay	1303:1317	The CCK-8 assay	1303:1317	The CCK-8 assay illustrated that AELP-B6 possessed higher cytotoxicity against HepG2 and HT-29 than that of AELP-B5.					
33436169	6	83	theme	molar	972:976	arg1	ratio					978:982	a distinguishing 1:1 molar ratio	951:982	a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3)	951:1238	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
33436169	9	84	theme	bioactivity	1636:1646	arg1	basis					1648:1652	a molecular structure and bioactivity basis	1610:1652	a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries	1610:1729	The above findings provide a molecular structure and bioactivity basis for future potential applications of AELP in the food and medical industries.					
33436169	8	85	theme	plasmon	1428:1434	arg1	resonance					1436:1444	Surface plasmon resonance	1420:1444	Surface plasmon resonance	1420:1444	Surface plasmon resonance showed the binding affinity of AELP-B6 to galectin-3 (6.488 × 10-5 M) was about 10 times stronger than that of AELP-B5 (4.588 × 10-4 M).					
33436169	6	86	dep	possessed	787:795	arg1	whereas					931:937	whereas	931:937	whereas	931:937	(1) AELP-B5 possessed →4GalA1→ as smooth regions (HG) and a repeating disaccharide moiety of →4GalA1→2Rha1→ as hairy regions (RG-I) with a 1:5 molar ratio, whereas AELP-B6 had a distinguishing 1:1 molar ratio between the HG and RG-I; (2) complex side chains were constituted of T-α-Araf, 1,3-α-Araf, 1,5-α-Araf, T-β-Galp, 1,3-β-Galp, 1,4-β-Galp, 1,6-β-Galp, 1,3,4-β-Galp and 1,3,4,6-β-Galp connected at C-4 of the rhamnosyl units in RG-I of AELP-B5 and B6; and (3) both possessed highly branched and compact coil conformations.					
34767672	4	0	located	present	831:837	arg2	glycoproteins					813:825	these special glycoproteins	799:825	these special glycoproteins	799:825	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	4	0	located	present	831:837	arg1	CGA					842:844	CGA	842:844	CGA	842:844	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	1	1	theme	plants	243:248	arg1	relatives					225:233	the closest relatives	213:233	the closest relatives of land plants	213:248	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	1	1	theme	plants	243:248	arg1	algae					182:186	Charophyte green algae	165:186	Charophyte green algae (CGA)	165:192	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	10	2	theme	glycan	1543:1548	arg1	part					1550:1553	the glycan part	1539:1553	the glycan part of βGlcY-precipitated molecules	1539:1585	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	10	3	theme	molecules	1577:1585	arg1	part					1550:1553	the glycan part	1539:1553	the glycan part of βGlcY-precipitated molecules	1539:1585	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	11	4	theme	pyranosidic	1786:1796	arg1	residues					1831:1838	pyranosidic terminal and 1,3-linked rhamnose residues	1786:1838	pyranosidic terminal and 1,3-linked rhamnose residues	1786:1838	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	1	5	from	processes	274:282	arg1	colonization					291:302	the colonization	287:302	the colonization of terrestrial habitats	287:326	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	3	6	theme	Spirogyra	558:566	arg1	walls					511:515	cell walls	506:515	cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae)	506:595	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	6	7	attach	present	990:996	arg1	wall					947:950	the cell wall	938:950	the cell wall of Nitellopsis	938:965	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	6	7	attach	present	990:996	arg2	hydroxyproline					971:984	no hydroxyproline	968:984	no hydroxyproline	968:984	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	10	8	theme	galactan	1605:1612	arg1	backbone					1614:1621	the galactan backbone	1601:1621	the galactan backbone	1601:1621	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	11	9	theme	rhamnose	1822:1829	arg1	residues					1831:1838	pyranosidic terminal and 1,3-linked rhamnose residues	1786:1838	pyranosidic terminal and 1,3-linked rhamnose residues	1786:1838	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	9	10	theme	growth	1443:1448	arg1	stages					1450:1455	generative growth stages	1432:1455	generative growth stages	1432:1455	Labelling increased strongly in generative growth stages, especially during zygospore development.					
34767672	11	11	link	1,3-linked	1811:1820	arg1	residues					1831:1838	pyranosidic terminal and 1,3-linked rhamnose residues	1786:1838	pyranosidic terminal and 1,3-linked rhamnose residues	1786:1838	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	4	12	gly	glycoproteins	813:825	arg1	glycoproteins					813:825	these special glycoproteins	799:825	these special glycoproteins	799:825	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	11	13	from	amounts	1724:1730	arg1	present					1702:1708	present	1702:1708	present	1702:1708	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	0	14	from	rhamnogalactan-protein	99:120	arg1	Zygnematophyceae					146:161	Zygnematophyceae	146:161	Zygnematophyceae	146:161	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	0	14	from	rhamnogalactan-protein	99:120	arg1	pratensis					135:143	pratensis	135:143	pratensis	135:143	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	12	15	from	S. pratensis	1933:1944	arg1	present					1922:1928	present	1922:1928	present	1922:1928	We introduce the term 'rhamnogalactan-protein' for this special AGP-modification present in S. pratensis.					
34767672	0	16	theme	evolutionary	11:22	arg1	roots					24:28	evolutionary roots	11:28	evolutionary roots of land plant arabinogalactan-proteins in charophytes	11:82	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	12	17	from	present	1922:1928	arg1	S. pratensis					1933:1944	S. pratensis	1933:1944	S. pratensis	1933:1944	We introduce the term 'rhamnogalactan-protein' for this special AGP-modification present in S. pratensis.					
34767672	11	18	from	present	1702:1708	arg1	amounts					1724:1730	small amounts	1718:1730	small amounts	1718:1730	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	6	19	from	present	990:996	arg1	wall					947:950	the cell wall	938:950	the cell wall of Nitellopsis	938:965	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	8	20	theme	non-conjugating	1281:1295	arg1	filaments					1307:1315	non-conjugating Spirogyra filaments	1281:1315	non-conjugating Spirogyra filaments	1281:1315	Putative AGPs in the cell walls of non-conjugating Spirogyra filaments, especially in the area of transverse walls, were detected by staining with βGlcY.					
34767672	12	21	theme	present	1922:1928	arg1	AGP-modification					1905:1920	this special AGP-modification	1892:1920	this special AGP-modification present in S. pratensis	1892:1944	We introduce the term 'rhamnogalactan-protein' for this special AGP-modification present in S. pratensis.					
34767672	1	22	theme	green	176:180	arg1	CGA					189:191	CGA	189:191	CGA	189:191	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	1	22	theme	green	176:180	arg1	relatives					225:233	the closest relatives	213:233	the closest relatives of land plants	213:248	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	1	22	theme	green	176:180	arg1	algae					182:186	Charophyte green algae	165:186	Charophyte green algae (CGA)	165:192	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	4	23	theme	cell	757:760	arg1	walls					762:766	all land plant cell walls	742:766	all land plant cell walls	742:766	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	6	24	located	present	990:996	arg2	hydroxyproline					971:984	no hydroxyproline	968:984	no hydroxyproline	968:984	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	6	24	located	present	990:996	arg1	wall					947:950	the cell wall	938:950	the cell wall of Nitellopsis	938:965	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	4	25	from	CGA	842:844	arg1	present					831:837	present	831:837	present	831:837	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	8	26	from	AGPs	1255:1258	arg1	walls					1272:1276	the cell walls	1263:1276	the cell walls of non-conjugating Spirogyra filaments	1263:1315	Putative AGPs in the cell walls of non-conjugating Spirogyra filaments, especially in the area of transverse walls, were detected by staining with βGlcY.					
34767672	8	26	from	AGPs	1255:1258	arg1	area					1336:1339	the area	1332:1339	the area of transverse walls	1332:1359	Putative AGPs in the cell walls of non-conjugating Spirogyra filaments, especially in the area of transverse walls, were detected by staining with βGlcY.					
34767672	6	27	theme	Nitellopsis	955:965	arg1	wall					947:950	the cell wall	938:950	the cell wall of Nitellopsis	938:965	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	10	28	theme	1,6-	1656:1659	arg1	moieties					1683:1690	1,3- 1,6- and 1,3,6-linked Galp moieties	1651:1690	1,3- 1,6- and 1,3,6-linked Galp moieties	1651:1690	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	11	29	located	present	1702:1708	arg1	amounts					1724:1730	small amounts	1718:1730	small amounts	1718:1730	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	11	29	located	present	1702:1708	arg2	Araf					1693:1696	Araf	1693:1696	Araf	1693:1696	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	2	30	theme	significant	382:392	arg1	changes					423:429	significant physiological and structural changes	382:429	significant physiological and structural changes	382:429	For the transition from water to land, plants needed significant physiological and structural changes, as well as with regard to cell wall composition.					
34767672	7	31	theme	Spirogyra	1147:1155	arg1	fraction					1135:1142	the water-soluble cell wall fraction	1107:1142	the water-soluble cell wall fraction of Spirogyra	1107:1155	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	4	32	from	present	831:837	arg1	CGA					842:844	CGA	842:844	CGA	842:844	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	3	33	from	overview	619:626	arg1	composition					641:651	cell wall composition	631:651	cell wall composition of late branching CGA	631:673	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	7	34	theme	fraction	1135:1142	arg1	precipitation					1090:1102	βGlcY precipitation	1084:1102	βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra	1084:1155	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	12	35	theme	special	1897:1903	arg1	AGP-modification					1905:1920	this special AGP-modification	1892:1920	this special AGP-modification present in S. pratensis	1892:1944	We introduce the term 'rhamnogalactan-protein' for this special AGP-modification present in S. pratensis.					
34767672	5	36	theme	AGPs	929:932	arg1	features					917:924	characteristic features	902:924	characteristic features of AGPs	902:932	Therefore, we investigated both species with regard to characteristic features of AGPs.					
34767672	4	37	theme	land	746:749	arg1	walls					762:766	all land plant cell walls	742:766	all land plant cell walls	742:766	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	7	38	theme	cell	1125:1128	arg1	fraction					1135:1142	the water-soluble cell wall fraction	1107:1142	the water-soluble cell wall fraction of Spirogyra	1107:1155	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	12	39	theme	term	1858:1861	arg1	rhamnogalactan-protein					1864:1885	the term 'rhamnogalactan-protein'	1854:1886	the term 'rhamnogalactan-protein' for this special AGP-modification present in S. pratensis	1854:1944	We introduce the term 'rhamnogalactan-protein' for this special AGP-modification present in S. pratensis.					
34767672	2	40	theme	cell	458:461	arg1	composition					468:478	cell wall composition	458:478	cell wall composition	458:478	For the transition from water to land, plants needed significant physiological and structural changes, as well as with regard to cell wall composition.					
34767672	8	41	theme	walls	1355:1359	arg1	area					1336:1339	the area	1332:1339	the area of transverse walls	1332:1359	Putative AGPs in the cell walls of non-conjugating Spirogyra filaments, especially in the area of transverse walls, were detected by staining with βGlcY.					
34767672	10	42	theme	1,3-	1651:1654	arg1	1,6-					1656:1659	1,3- 1,6-	1651:1659	1,3- 1,6-	1651:1659	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	3	43	theme	cell	631:634	arg1	composition					641:651	cell wall composition	631:651	cell wall composition of late branching CGA	631:673	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	0	44	theme	plant	38:42	arg1	arabinogalactan-proteins					44:67	land plant arabinogalactan-proteins	33:67	land plant arabinogalactan-proteins in charophytes	33:82	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	1	45	theme	terrestrial	307:317	arg1	habitats					319:326	terrestrial habitats	307:326	terrestrial habitats	307:326	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	3	46	theme	CGA	671:673	arg1	composition					641:651	cell wall composition	631:651	cell wall composition of late branching CGA	631:673	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	10	47	theme	Galp	1678:1681	arg1	moieties					1683:1690	1,3- 1,6- and 1,3,6-linked Galp moieties	1651:1690	1,3- 1,6- and 1,3,6-linked Galp moieties	1651:1690	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	10	48	theme	structure	1526:1534	arg1	Investigations					1499:1512	Investigations	1499:1512	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules	1499:1585	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	2	49	theme	physiological	394:406	arg1	changes					423:429	significant physiological and structural changes	382:429	significant physiological and structural changes	382:429	For the transition from water to land, plants needed significant physiological and structural changes, as well as with regard to cell wall composition.					
34767672	3	50	theme	cell	506:509	arg1	walls					511:515	cell walls	506:515	cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae)	506:595	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	7	51	theme	βGlcY	1084:1088	arg1	precipitation					1090:1102	βGlcY precipitation	1084:1102	βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra	1084:1155	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	0	52	dep	Search	0:5	arg1	presence					85:92	presence	85:92	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).	0:163	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	10	53	link	1,3,6-linked	1665:1676	arg1	Galp					1678:1681	1,3,6-linked Galp	1665:1681	1,3,6-linked Galp	1665:1681	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	1	54	theme	land	238:241	arg1	plants					243:248	land plants	238:248	land plants	238:248	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	10	55	with	AGPs	1641:1644	arg1	moieties					1683:1690	1,3- 1,6- and 1,3,6-linked Galp moieties	1651:1690	1,3- 1,6- and 1,3,6-linked Galp moieties	1651:1690	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	7	56	from	hydroxyproline	1197:1210	arg1	rich					1189:1192	rich	1189:1192	rich	1189:1192	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	10	57	theme	part	1550:1553	arg1	structure					1526:1534	the fine structure	1517:1534	the fine structure of the glycan part of βGlcY-precipitated molecules	1517:1585	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	11	58	attach	present	1702:1708	arg1	amounts					1724:1730	small amounts	1718:1730	small amounts	1718:1730	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	11	58	attach	present	1702:1708	arg2	Araf					1693:1696	Araf	1693:1696	Araf	1693:1696	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	2	59	from	water	353:357	arg1	transition					337:346	the transition	333:346	the transition from water to land	333:365	For the transition from water to land, plants needed significant physiological and structural changes, as well as with regard to cell wall composition.					
34767672	10	60	theme	βGlcY-precipitated	1558:1575	arg1	molecules					1577:1585	βGlcY-precipitated molecules	1558:1585	βGlcY-precipitated molecules	1558:1585	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	11	61	theme	terminal	1798:1805	arg1	residues					1831:1838	pyranosidic terminal and 1,3-linked rhamnose residues	1786:1838	pyranosidic terminal and 1,3-linked rhamnose residues	1786:1838	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	3	62	theme	Charophyceae	540:551	arg1	walls					511:515	cell walls	506:515	cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae)	506:595	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	6	63	theme	cell	942:945	arg1	wall					947:950	the cell wall	938:950	the cell wall of Nitellopsis	938:965	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	0	64	from	presence	85:92	arg1	Zygnematophyceae					146:161	Zygnematophyceae	146:161	Zygnematophyceae	146:161	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	0	64	from	presence	85:92	arg1	pratensis					135:143	pratensis	135:143	pratensis	135:143	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	3	65	dep	Spirogyra	558:566	arg1	pratensis					568:576	Spirogyra pratensis (Zygnematophyceae)	558:595	Spirogyra pratensis (Zygnematophyceae)	558:595	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	9	66	theme	generative	1432:1441	arg1	stages					1450:1455	generative growth stages	1432:1455	generative growth stages	1432:1455	Labelling increased strongly in generative growth stages, especially during zygospore development.					
34767672	0	67	from	charophytes	72:82	arg1	roots					24:28	evolutionary roots	11:28	evolutionary roots of land plant arabinogalactan-proteins in charophytes	11:82	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	8	68	theme	Putative	1246:1253	arg1	AGPs					1255:1258	Putative AGPs	1246:1258	Putative AGPs	1246:1258	Putative AGPs in the cell walls of non-conjugating Spirogyra filaments, especially in the area of transverse walls, were detected by staining with βGlcY.					
34767672	11	69	theme	1,3-linked	1811:1820	arg1	residues					1831:1838	pyranosidic terminal and 1,3-linked rhamnose residues	1786:1838	pyranosidic terminal and 1,3-linked rhamnose residues	1786:1838	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	3	70	theme	comparative	607:617	arg1	overview					619:626	a comparative overview	605:626	a comparative overview on cell wall composition of late branching CGA	605:673	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	5	71	theme	characteristic	902:915	arg1	features					917:924	characteristic features	902:924	characteristic features of AGPs	902:932	Therefore, we investigated both species with regard to characteristic features of AGPs.					
34767672	7	72	from	rich	1189:1192	arg1	hydroxyproline					1197:1210	hydroxyproline	1197:1210	hydroxyproline	1197:1210	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	8	73	theme	filaments	1307:1315	arg1	walls					1272:1276	the cell walls	1263:1276	the cell walls of non-conjugating Spirogyra filaments	1263:1315	Putative AGPs in the cell walls of non-conjugating Spirogyra filaments, especially in the area of transverse walls, were detected by staining with βGlcY.					
34767672	7	74	theme	rich	1189:1192	arg1	fraction					1180:1187	a glycoprotein fraction	1165:1187	a glycoprotein fraction rich in hydroxyproline	1165:1210	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	9	75	theme	zygospore	1476:1484	arg1	development					1486:1496	zygospore development	1476:1496	zygospore development	1476:1496	Labelling increased strongly in generative growth stages, especially during zygospore development.					
34767672	0	76	from	roots	24:28	arg1	charophytes					72:82	charophytes	72:82	charophytes	72:82	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	8	77	theme	cell	1267:1270	arg1	walls					1272:1276	the cell walls	1263:1276	the cell walls of non-conjugating Spirogyra filaments	1263:1315	Putative AGPs in the cell walls of non-conjugating Spirogyra filaments, especially in the area of transverse walls, were detected by staining with βGlcY.					
34767672	11	78	theme	small	1718:1722	arg1	amounts					1724:1730	small amounts	1718:1730	small amounts	1718:1730	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	7	79	theme	glycoprotein	1167:1178	arg1	fraction					1180:1187	a glycoprotein fraction	1165:1187	a glycoprotein fraction rich in hydroxyproline	1165:1210	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	1	80	theme	Charophyte	165:174	arg1	CGA					189:191	CGA	189:191	CGA	189:191	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	1	80	theme	Charophyte	165:174	arg1	relatives					225:233	the closest relatives	213:233	the closest relatives of land plants	213:248	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	1	80	theme	Charophyte	165:174	arg1	algae					182:186	Charophyte green algae	165:186	Charophyte green algae (CGA)	165:192	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	4	81	attach	present	831:837	arg2	glycoproteins					813:825	these special glycoproteins	799:825	these special glycoproteins	799:825	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	4	81	attach	present	831:837	arg1	CGA					842:844	CGA	842:844	CGA	842:844	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	2	82	theme	wall	463:466	arg1	composition					468:478	cell wall composition	458:478	cell wall composition	458:478	For the transition from water to land, plants needed significant physiological and structural changes, as well as with regard to cell wall composition.					
34767672	0	83	from	arabinogalactan-proteins	44:67	arg1	charophytes					72:82	charophytes	72:82	charophytes	72:82	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	4	84	theme	plant	751:755	arg1	walls					762:766	all land plant cell walls	742:766	all land plant cell walls	742:766	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	8	85	theme	Spirogyra	1297:1305	arg1	filaments					1307:1315	non-conjugating Spirogyra filaments	1281:1315	non-conjugating Spirogyra filaments	1281:1315	Putative AGPs in the cell walls of non-conjugating Spirogyra filaments, especially in the area of transverse walls, were detected by staining with βGlcY.					
34767672	11	86	theme	terminating	1740:1750	arg1	sugars					1752:1757	the terminating sugars	1736:1757	the terminating sugars	1736:1757	Araf was present only in small amounts and the terminating sugars consisted predominantly of pyranosidic terminal and 1,3-linked rhamnose residues.					
34767672	7	87	theme	wall	1130:1133	arg1	fraction					1135:1142	the water-soluble cell wall fraction	1107:1142	the water-soluble cell wall fraction of Spirogyra	1107:1155	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	6	88	theme	β-glucosyl	1035:1044	arg1	Yariv					1046:1050	the β-glucosyl Yariv's	1031:1052	the β-glucosyl Yariv's reagent (βGlcY)	1031:1068	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	3	89	theme	late	656:659	arg1	CGA					671:673	late branching CGA	656:673	late branching CGA	656:673	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	7	90	theme	water-soluble	1111:1123	arg1	fraction					1135:1142	the water-soluble cell wall fraction	1107:1142	the water-soluble cell wall fraction of Spirogyra	1107:1155	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	3	91	theme	wall	636:639	arg1	composition					641:651	cell wall composition	631:651	cell wall composition of late branching CGA	631:673	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	7	92	gly	glycoprotein	1167:1178	arg1	glycoprotein					1167:1178	a glycoprotein fraction	1165:1187	a glycoprotein fraction rich in hydroxyproline	1165:1210	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	2	93	theme	structural	412:421	arg1	changes					423:429	significant physiological and structural changes	382:429	significant physiological and structural changes	382:429	For the transition from water to land, plants needed significant physiological and structural changes, as well as with regard to cell wall composition.					
34767672	0	94	theme	land	33:36	arg1	arabinogalactan-proteins					44:67	land plant arabinogalactan-proteins	33:67	land plant arabinogalactan-proteins in charophytes	33:82	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	12	95	attach	present	1922:1928	arg1	S. pratensis					1933:1944	S. pratensis	1933:1944	S. pratensis	1933:1944	We introduce the term 'rhamnogalactan-protein' for this special AGP-modification present in S. pratensis.					
34767672	12	95	attach	present	1922:1928	arg2	AGP-modification					1905:1920	this special AGP-modification	1892:1920	this special AGP-modification present in S. pratensis	1892:1944	We introduce the term 'rhamnogalactan-protein' for this special AGP-modification present in S. pratensis.					
34767672	6	96	from	wall	947:950	arg1	present					990:996	present	990:996	present	990:996	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	8	97	theme	transverse	1344:1353	arg1	walls					1355:1359	transverse walls	1344:1359	transverse walls	1344:1359	Putative AGPs in the cell walls of non-conjugating Spirogyra filaments, especially in the area of transverse walls, were detected by staining with βGlcY.					
34767672	0	98	theme	rhamnogalactan-protein	99:120	arg1	presence					85:92	presence	85:92	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).	0:163	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	0	99	theme	arabinogalactan-proteins	44:67	arg1	roots					24:28	evolutionary roots	11:28	evolutionary roots of land plant arabinogalactan-proteins in charophytes	11:82	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	7	100	theme	AGPs	1240:1243	arg1	presence					1228:1235	the presence	1224:1235	the presence of AGPs	1224:1243	By contrast, βGlcY precipitation of the water-soluble cell wall fraction of Spirogyra yielded a glycoprotein fraction rich in hydroxyproline, indicating the presence of AGPs.					
34767672	3	101	theme	branching	661:669	arg1	CGA					671:673	late branching CGA	656:673	late branching CGA	656:673	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	4	102	theme	special	805:811	arg1	glycoproteins					813:825	these special glycoproteins	799:825	these special glycoproteins	799:825	Because arabinogalactan-proteins (AGPs) are considered common for all land plant cell walls, we were interested in whether these special glycoproteins are present in CGA.					
34767672	3	103	theme	Sequential	481:490	arg1	extraction					492:501	Sequential extraction	481:501	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae)	481:595	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
34767672	1	104	theme	habitats	319:326	arg1	colonization					291:302	the colonization	287:302	the colonization of terrestrial habitats	287:326	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	10	105	theme	1,3,6-linked	1665:1676	arg1	Galp					1678:1681	1,3,6-linked Galp	1665:1681	1,3,6-linked Galp	1665:1681	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	1	106	theme	closest	217:223	arg1	relatives					225:233	the closest relatives	213:233	the closest relatives of land plants	213:248	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	1	106	theme	closest	217:223	arg1	algae					182:186	Charophyte green algae	165:186	Charophyte green algae (CGA)	165:192	Charophyte green algae (CGA) are assigned to be the closest relatives of land plants and therefore enlighten processes in the colonization of terrestrial habitats.					
34767672	6	107	with	precipitable	1013:1024	arg1	βGlcY					1063:1067	βGlcY	1063:1067	βGlcY	1063:1067	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	6	107	with	precipitable	1013:1024	arg1	reagent					1054:1060	the β-glucosyl Yariv's reagent	1031:1060	the β-glucosyl Yariv's reagent (βGlcY)	1031:1068	In the cell wall of Nitellopsis, no hydroxyproline was present and no AGP was precipitable with the β-glucosyl Yariv's reagent (βGlcY).					
34767672	0	108	from	pratensis	135:143	arg1	presence					85:92	presence	85:92	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).	0:163	Search for evolutionary roots of land plant arabinogalactan-proteins in charophytes: presence of a rhamnogalactan-protein in Spirogyra pratensis (Zygnematophyceae).					
34767672	10	109	theme	fine	1521:1524	arg1	structure					1526:1534	the fine structure	1517:1534	the fine structure of the glycan part of βGlcY-precipitated molecules	1517:1585	Investigations of the fine structure of the glycan part of βGlcY-precipitated molecules revealed that the galactan backbone resembled that of AGPs with 1,3- 1,6- and 1,3,6-linked Galp moieties.					
34767672	3	110	theme	walls	511:515	arg1	extraction					492:501	Sequential extraction	481:501	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae)	481:595	Sequential extraction of cell walls of Nitellopsis obtusa (Charophyceae) and Spirogyra pratensis (Zygnematophyceae) offered a comparative overview on cell wall composition of late branching CGA.					
33858302	5	0	theme	chemical	679:686	arg1	composition					688:698	chemical composition	679:698	chemical composition	679:698	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	7	1	theme	zeta	925:928	arg1	potential					930:938	zeta potential	925:938	zeta potential	925:938	The FTIR and zeta potential analyses exhibited that the amino groups (-NH2) were grafted on MDE, and the surface potential of diatomite altered from -24 mV to 55 mV.					
33858302	11	2	theme	tissue	1640:1645	arg1	regeneration					1647:1658	the bone tissue regeneration	1631:1658	the bone tissue regeneration	1631:1658	The findings obtained in this study reveal that the MDE-rhBMP-2/CS composite scaffold can be potentially used to promote the bone tissue regeneration.					
33858302	10	3	theme	stem	1442:1445	arg1	cells					1447:1451	the bone mesenchymal stem cells	1421:1451	the bone mesenchymal stem cells	1421:1451	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	10	4	theme	regeneration	1487:1498	arg1	capacity					1500:1507	an optimal bone regeneration capacity	1471:1507	an optimal bone regeneration capacity	1471:1507	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	4	5	theme	diatomite	594:602	arg1	surface					583:589	the surface	579:589	the surface of diatomite via hydroxyl groups	579:622	Specifically, modified-diatomite (MDE) was synthesized by grafting polyethyleneimine (PEI) on the surface of diatomite via hydroxyl groups.					
33858302	10	6	theme	bone	1425:1428	arg1	cells					1447:1451	the bone mesenchymal stem cells	1421:1451	the bone mesenchymal stem cells	1421:1451	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	10	7	theme	cells	1447:1451	arg1	proliferation					1373:1385	proliferation	1373:1385	proliferation	1373:1385	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	10	7	theme	cells	1447:1451	arg1	differentiation					1402:1416	osteogenic differentiation	1391:1416	osteogenic differentiation	1391:1416	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	6	8	theme	activity	850:857	arg1	analyses					859:866	osteogenic activity analyses	839:866	osteogenic activity analyses	839:866	Further, the mechanical strength, drug release, cytotoxicity and osteogenic activity analyses were carried out for the scaffold material.					
33858302	0	9	theme	BMP-2	104:108	arg1	release					93:99	sustained release	83:99	sustained release of BMP-2	83:108	PEI-modified diatomite/chitosan composites as bone tissue engineering scaffold for sustained release of BMP-2.					
33858302	5	10	theme	zeta	701:704	arg1	potential					706:714	zeta potential	701:714	zeta potential	701:714	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	5	11	theme	physicochemical	629:643	arg1	composition					688:698	chemical composition	679:698	chemical composition	679:698	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	5	11	theme	physicochemical	629:643	arg1	potential					706:714	zeta potential	701:714	zeta potential	701:714	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	5	11	theme	physicochemical	629:643	arg1	behavior					732:739	adsorption behavior	721:739	adsorption behavior	721:739	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	5	11	theme	physicochemical	629:643	arg1	characteristics					645:659	The physicochemical characteristics	625:659	The physicochemical characteristics	625:659	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	11	12	theme	composite	1577:1585	arg1	scaffold					1587:1594	the MDE-rhBMP-2/CS composite scaffold	1558:1594	the MDE-rhBMP-2/CS composite scaffold	1558:1594	The findings obtained in this study reveal that the MDE-rhBMP-2/CS composite scaffold can be potentially used to promote the bone tissue regeneration.					
33858302	4	13	theme	hydroxyl	608:615	arg1	groups					617:622	hydroxyl groups	608:622	hydroxyl groups	608:622	Specifically, modified-diatomite (MDE) was synthesized by grafting polyethyleneimine (PEI) on the surface of diatomite via hydroxyl groups.					
33858302	3	14	theme	chitosan-based	365:378	arg1	scaffold					380:387	a novel chitosan-based scaffold	357:387	a novel chitosan-based scaffold	357:387	In this study, a novel chitosan-based scaffold was prepared by incorporating modified natural diatomite (DE) as filler and adsorption element.					
33858302	10	15	theme	optimal	1474:1480	arg1	capacity					1500:1507	an optimal bone regeneration capacity	1471:1507	an optimal bone regeneration capacity	1471:1507	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	1	16	theme	significant	173:183	arg1	challenge					194:202	a significant clinical challenge	171:202	a significant clinical challenge	171:202	The bone healing defects resulting from bone disease remain a significant clinical challenge.					
33858302	5	17	theme	MDE	664:666	arg1	composition					688:698	chemical composition	679:698	chemical composition	679:698	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	5	17	theme	MDE	664:666	arg1	potential					706:714	zeta potential	701:714	zeta potential	701:714	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	5	17	theme	MDE	664:666	arg1	behavior					732:739	adsorption behavior	721:739	adsorption behavior	721:739	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	5	17	theme	MDE	664:666	arg1	characteristics					645:659	The physicochemical characteristics	625:659	The physicochemical characteristics	625:659	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	9	18	theme	compressive	1205:1215	arg1	strength					1217:1224	The compressive strength	1201:1224	The compressive strength	1201:1224	The compressive strength was observed to be enhanced due to the addition of MDE.					
33858302	0	19	theme	PEI-modified	0:11	arg1	composites					32:41	PEI-modified diatomite/chitosan composites	0:41	PEI-modified diatomite/chitosan composites as bone tissue engineering scaffold for sustained release of BMP-2.	0:109	PEI-modified diatomite/chitosan composites as bone tissue engineering scaffold for sustained release of BMP-2.					
33858302	1	20	theme	clinical	185:192	arg1	challenge					194:202	a significant clinical challenge	171:202	a significant clinical challenge	171:202	The bone healing defects resulting from bone disease remain a significant clinical challenge.					
33858302	3	21	theme	modified	419:426	arg1	DE					447:448	DE	447:448	DE	447:448	In this study, a novel chitosan-based scaffold was prepared by incorporating modified natural diatomite (DE) as filler and adsorption element.					
33858302	3	21	theme	modified	419:426	arg1	diatomite					436:444	modified natural diatomite	419:444	modified natural diatomite (DE)	419:449	In this study, a novel chitosan-based scaffold was prepared by incorporating modified natural diatomite (DE) as filler and adsorption element.					
33858302	10	22	theme	mesenchymal	1430:1440	arg1	cells					1447:1451	the bone mesenchymal stem cells	1421:1451	the bone mesenchymal stem cells	1421:1451	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	7	23	dep	FTIR	916:919	arg1	analyses					940:947	analyses	940:947	analyses	940:947	The FTIR and zeta potential analyses exhibited that the amino groups (-NH2) were grafted on MDE, and the surface potential of diatomite altered from -24 mV to 55 mV.					
33858302	11	24	used	used	1615:1618	arg2	scaffold					1587:1594	the MDE-rhBMP-2/CS composite scaffold	1558:1594	the MDE-rhBMP-2/CS composite scaffold	1558:1594	The findings obtained in this study reveal that the MDE-rhBMP-2/CS composite scaffold can be potentially used to promote the bone tissue regeneration.					
33858302	3	25	theme	natural	428:434	arg1	DE					447:448	DE	447:448	DE	447:448	In this study, a novel chitosan-based scaffold was prepared by incorporating modified natural diatomite (DE) as filler and adsorption element.					
33858302	3	25	theme	natural	428:434	arg1	diatomite					436:444	modified natural diatomite	419:444	modified natural diatomite (DE)	419:449	In this study, a novel chitosan-based scaffold was prepared by incorporating modified natural diatomite (DE) as filler and adsorption element.					
33858302	10	26	theme	bone	1482:1485	arg1	capacity					1500:1507	an optimal bone regeneration capacity	1471:1507	an optimal bone regeneration capacity	1471:1507	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	6	27	theme	drug	808:811	arg1	release					813:819	drug release	808:819	drug release	808:819	Further, the mechanical strength, drug release, cytotoxicity and osteogenic activity analyses were carried out for the scaffold material.					
33858302	1	28	theme	bone	115:118	arg1	defects					128:134	The bone healing defects	111:134	The bone healing defects resulting from bone disease	111:162	The bone healing defects resulting from bone disease remain a significant clinical challenge.					
33858302	10	29	theme	composite	1295:1303	arg1	scaffold					1305:1312	the composite scaffold	1291:1312	the composite scaffold loaded with rhBMP-2	1291:1332	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	0	30	theme	tissue	51:56	arg1	scaffold					70:77	bone tissue engineering scaffold	46:77	bone tissue engineering scaffold for sustained release of BMP-2	46:108	PEI-modified diatomite/chitosan composites as bone tissue engineering scaffold for sustained release of BMP-2.					
33858302	8	31	theme	adsorption	1104:1113	arg1	capacity					1115:1122	the protein adsorption capacity	1092:1122	the protein adsorption capacity	1092:1122	Subsequently, the protein adsorption capacity and cytocompatibility of MDE were observed to be improved as compared to DE.					
33858302	2	32	theme	osteoinductive	257:270	arg1	compounds					272:280	osteoinductive compounds	257:280	osteoinductive compounds	257:280	The bone tissue engineering scaffolds combined with osteoinductive compounds represent an effective approach to overcome this challenge.					
33858302	0	33	theme	bone	46:49	arg1	scaffold					70:77	bone tissue engineering scaffold	46:77	bone tissue engineering scaffold for sustained release of BMP-2	46:108	PEI-modified diatomite/chitosan composites as bone tissue engineering scaffold for sustained release of BMP-2.					
33858302	8	34	theme	protein	1096:1102	arg1	capacity					1115:1122	the protein adsorption capacity	1092:1122	the protein adsorption capacity	1092:1122	Subsequently, the protein adsorption capacity and cytocompatibility of MDE were observed to be improved as compared to DE.					
33858302	6	35	theme	scaffold	893:900	arg1	material					902:909	the scaffold material	889:909	the scaffold material	889:909	Further, the mechanical strength, drug release, cytotoxicity and osteogenic activity analyses were carried out for the scaffold material.					
33858302	10	36	from	impact	1363:1368	arg1	proliferation					1373:1385	proliferation	1373:1385	proliferation	1373:1385	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	10	36	from	impact	1363:1368	arg1	differentiation					1402:1416	osteogenic differentiation	1391:1416	osteogenic differentiation	1391:1416	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	6	37	theme	osteogenic	839:848	arg1	analyses					859:866	osteogenic activity analyses	839:866	osteogenic activity analyses	839:866	Further, the mechanical strength, drug release, cytotoxicity and osteogenic activity analyses were carried out for the scaffold material.					
33858302	8	38	theme	MDE	1149:1151	arg1	cytocompatibility					1128:1144	cytocompatibility	1128:1144	cytocompatibility	1128:1144	Subsequently, the protein adsorption capacity and cytocompatibility of MDE were observed to be improved as compared to DE.					
33858302	8	38	theme	MDE	1149:1151	arg1	capacity					1115:1122	the protein adsorption capacity	1092:1122	the protein adsorption capacity	1092:1122	Subsequently, the protein adsorption capacity and cytocompatibility of MDE were observed to be improved as compared to DE.					
33858302	1	39	theme	healing	120:126	arg1	defects					128:134	The bone healing defects	111:134	The bone healing defects resulting from bone disease	111:162	The bone healing defects resulting from bone disease remain a significant clinical challenge.					
33858302	0	40	theme	engineering	58:68	arg1	scaffold					70:77	bone tissue engineering scaffold	46:77	bone tissue engineering scaffold for sustained release of BMP-2	46:108	PEI-modified diatomite/chitosan composites as bone tissue engineering scaffold for sustained release of BMP-2.					
33858302	10	41	theme	osteogenic	1391:1400	arg1	differentiation					1402:1416	osteogenic differentiation	1391:1416	osteogenic differentiation	1391:1416	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	2	42	theme	engineering	221:231	arg1	scaffolds					233:241	The bone tissue engineering scaffolds	205:241	The bone tissue engineering scaffolds combined with osteoinductive compounds	205:280	The bone tissue engineering scaffolds combined with osteoinductive compounds represent an effective approach to overcome this challenge.					
33858302	6	43	theme	mechanical	787:796	arg1	strength					798:805	the mechanical strength	783:805	the mechanical strength	783:805	Further, the mechanical strength, drug release, cytotoxicity and osteogenic activity analyses were carried out for the scaffold material.					
33858302	7	44	theme	amino	968:972	arg1	groups					974:979	the amino groups	964:979	the amino groups (-NH2)	964:986	The FTIR and zeta potential analyses exhibited that the amino groups (-NH2) were grafted on MDE, and the surface potential of diatomite altered from -24 mV to 55 mV.					
33858302	7	44	theme	amino	968:972	arg1	-NH2					982:985	-NH2	982:985	-NH2	982:985	The FTIR and zeta potential analyses exhibited that the amino groups (-NH2) were grafted on MDE, and the surface potential of diatomite altered from -24 mV to 55 mV.					
33858302	7	45	theme	diatomite	1038:1046	arg1	potential					1025:1033	the surface potential	1013:1033	the surface potential of diatomite	1013:1046	The FTIR and zeta potential analyses exhibited that the amino groups (-NH2) were grafted on MDE, and the surface potential of diatomite altered from -24 mV to 55 mV.					
33858302	2	46	theme	tissue	214:219	arg1	scaffolds					233:241	The bone tissue engineering scaffolds	205:241	The bone tissue engineering scaffolds combined with osteoinductive compounds	205:280	The bone tissue engineering scaffolds combined with osteoinductive compounds represent an effective approach to overcome this challenge.					
33858302	2	47	theme	effective	295:303	arg1	approach					305:312	an effective approach	292:312	an effective approach to overcome this challenge	292:339	The bone tissue engineering scaffolds combined with osteoinductive compounds represent an effective approach to overcome this challenge.					
33858302	7	48	theme	surface	1017:1023	arg1	potential					1025:1033	the surface potential	1013:1033	the surface potential of diatomite	1013:1046	The FTIR and zeta potential analyses exhibited that the amino groups (-NH2) were grafted on MDE, and the surface potential of diatomite altered from -24 mV to 55 mV.					
33858302	11	49	theme	MDE-rhBMP-2/CS	1562:1575	arg1	scaffold					1587:1594	the MDE-rhBMP-2/CS composite scaffold	1558:1594	the MDE-rhBMP-2/CS composite scaffold	1558:1594	The findings obtained in this study reveal that the MDE-rhBMP-2/CS composite scaffold can be potentially used to promote the bone tissue regeneration.					
33858302	5	50	theme	adsorption	721:730	arg1	behavior					732:739	adsorption behavior	721:739	adsorption behavior	721:739	The physicochemical characteristics of MDE, including chemical composition, zeta potential, and adsorption behavior, were investigated successively.					
33858302	2	51	theme	bone	209:212	arg1	scaffolds					233:241	The bone tissue engineering scaffolds	205:241	The bone tissue engineering scaffolds combined with osteoinductive compounds	205:280	The bone tissue engineering scaffolds combined with osteoinductive compounds represent an effective approach to overcome this challenge.					
33858302	0	52	theme	sustained	83:91	arg1	release					93:99	sustained release	83:99	sustained release of BMP-2	83:108	PEI-modified diatomite/chitosan composites as bone tissue engineering scaffold for sustained release of BMP-2.					
33858302	11	53	theme	bone	1635:1638	arg1	regeneration					1647:1658	the bone tissue regeneration	1631:1658	the bone tissue regeneration	1631:1658	The findings obtained in this study reveal that the MDE-rhBMP-2/CS composite scaffold can be potentially used to promote the bone tissue regeneration.					
33858302	1	54	theme	bone	151:154	arg1	disease					156:162	bone disease	151:162	bone disease	151:162	The bone healing defects resulting from bone disease remain a significant clinical challenge.					
33858302	3	55	theme	adsorption	465:474	arg1	element					476:482	adsorption element	465:482	adsorption element	465:482	In this study, a novel chitosan-based scaffold was prepared by incorporating modified natural diatomite (DE) as filler and adsorption element.					
33858302	3	56	theme	novel	359:363	arg1	scaffold					380:387	a novel chitosan-based scaffold	357:387	a novel chitosan-based scaffold	357:387	In this study, a novel chitosan-based scaffold was prepared by incorporating modified natural diatomite (DE) as filler and adsorption element.					
33858302	9	57	theme	MDE	1277:1279	arg1	addition					1265:1272	the addition	1261:1272	the addition of MDE	1261:1279	The compressive strength was observed to be enhanced due to the addition of MDE.					
33858302	10	58	theme	positive	1354:1361	arg1	impact					1363:1368	a more positive impact	1347:1368	a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells	1347:1451	Besides, the composite scaffold loaded with rhBMP-2 demonstrated a more positive impact on proliferation and osteogenic differentiation of the bone mesenchymal stem cells, thus, indicating an optimal bone regeneration capacity.					
33858302	0	59	theme	diatomite/chitosan	13:30	arg1	composites					32:41	PEI-modified diatomite/chitosan composites	0:41	PEI-modified diatomite/chitosan composites as bone tissue engineering scaffold for sustained release of BMP-2.	0:109	PEI-modified diatomite/chitosan composites as bone tissue engineering scaffold for sustained release of BMP-2.					
33483080	5	0	theme	corresponding	688:700	arg1	properties					741:750	The corresponding structural, thermal, and morphological properties	684:750	The corresponding structural, thermal, and morphological properties of hydrogels	684:763	The corresponding structural, thermal, and morphological properties of hydrogels were characterized.					
33483080	1	1	theme	reversible	210:219	arg1	interaction					236:246	reversible supramolecular interaction	210:246	reversible supramolecular interaction	210:246	Most structural self-healing materials were developed based on either reversible supramolecular interaction or dynamic covalent bonding.					
33483080	0	2	theme	self-healing	115:126	arg1	properties					128:137	self-healing properties	115:137	self-healing properties	115:137	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide interpenetrating network hydrogel with self-healing properties.					
33483080	5	3	theme	hydrogels	755:763	arg1	properties					741:750	The corresponding structural, thermal, and morphological properties	684:750	The corresponding structural, thermal, and morphological properties of hydrogels	684:763	The corresponding structural, thermal, and morphological properties of hydrogels were characterized.					
33483080	1	4	theme	supramolecular	221:234	arg1	interaction					236:246	reversible supramolecular interaction	210:246	reversible supramolecular interaction	210:246	Most structural self-healing materials were developed based on either reversible supramolecular interaction or dynamic covalent bonding.					
33483080	6	5	theme	reversible	789:798	arg1	transformation					800:813	The reversible transformation	785:813	The reversible transformation of the sol-gel	785:828	The reversible transformation of the sol-gel can be achieved by the irradiation upon ultraviolet light and visible light.					
33483080	6	6	theme	visible	892:898	arg1	light					900:904	visible light	892:904	visible light	892:904	The reversible transformation of the sol-gel can be achieved by the irradiation upon ultraviolet light and visible light.					
33483080	8	7	theme	%	1142:1142	arg1	elongation					1123:1132	an elongation	1120:1132	an elongation of 1140 %	1120:1142	The recovery gel elongation at 48 h healing in the dark condition was is 0.04 MPa, with an elongation of 1140 %.					
33483080	3	8	with	hydrogel	517:524	arg1	behavior					546:553	self-repairing behavior	531:553	self-repairing behavior under light irradiation	531:577	In this study, we fabricated the alginate-based cyclodextrin and polyacrylamide azobenzene highly stretchable and tough interpenetrating composite hydrogel with self-repairing behavior under light irradiation.					
33483080	9	9	theme	stretchable	1223:1233	arg1	performance					1245:1255	highly stretchable and tough performance	1216:1255	highly stretchable and tough performance	1216:1255	Therefore, this gel can achieve self-healing ability while maintaining highly stretchable and tough performance.					
33483080	4	10	theme	alginate-based	595:608	arg1	cyclodextrin					610:621	the alginate-based cyclodextrin	591:621	the alginate-based cyclodextrin	591:621	Initially, the alginate-based cyclodextrin and polyacrylamide azobenzene were designed and synthesized.					
33483080	3	11	theme	light	561:565	arg1	irradiation					567:577	light irradiation	561:577	light irradiation	561:577	In this study, we fabricated the alginate-based cyclodextrin and polyacrylamide azobenzene highly stretchable and tough interpenetrating composite hydrogel with self-repairing behavior under light irradiation.					
33483080	3	12	theme	polyacrylamide	435:448	arg1	azobenzene					450:459	polyacrylamide azobenzene	435:459	polyacrylamide azobenzene	435:459	In this study, we fabricated the alginate-based cyclodextrin and polyacrylamide azobenzene highly stretchable and tough interpenetrating composite hydrogel with self-repairing behavior under light irradiation.					
33483080	4	13	theme	polyacrylamide	627:640	arg1	azobenzene					642:651	polyacrylamide azobenzene	627:651	polyacrylamide azobenzene	627:651	Initially, the alginate-based cyclodextrin and polyacrylamide azobenzene were designed and synthesized.					
33483080	2	14	theme	high-performance	342:357	arg1	materials					359:367	high-performance materials	342:367	high-performance materials	342:367	It seems a good idea to incorporate self-healing properties into high-performance materials.					
33483080	6	15	theme	ultraviolet	870:880	arg1	light					882:886	ultraviolet light	870:886	ultraviolet light	870:886	The reversible transformation of the sol-gel can be achieved by the irradiation upon ultraviolet light and visible light.					
33483080	0	16	theme	stretchable	7:17	arg1	cyclodextrin/Azo-polyacrylamide					44:74	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide	0:74	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide	0:74	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide interpenetrating network hydrogel with self-healing properties.					
33483080	5	17	theme	morphological	727:739	arg1	properties					741:750	The corresponding structural, thermal, and morphological properties	684:750	The corresponding structural, thermal, and morphological properties of hydrogels	684:763	The corresponding structural, thermal, and morphological properties of hydrogels were characterized.					
33483080	8	18	theme	48	1063:1064	arg1	h					1066:1066	h	1066:1066	h	1066:1066	The recovery gel elongation at 48 h healing in the dark condition was is 0.04 MPa, with an elongation of 1140 %.					
33483080	1	19	theme	dynamic	251:257	arg1	bonding					268:274	dynamic covalent bonding	251:274	dynamic covalent bonding	251:274	Most structural self-healing materials were developed based on either reversible supramolecular interaction or dynamic covalent bonding.					
33483080	3	20	theme	interpenetrating	490:505	arg1	hydrogel					517:524	interpenetrating composite hydrogel	490:524	interpenetrating composite hydrogel with self-repairing behavior under light irradiation	490:577	In this study, we fabricated the alginate-based cyclodextrin and polyacrylamide azobenzene highly stretchable and tough interpenetrating composite hydrogel with self-repairing behavior under light irradiation.					
33483080	7	21	theme	host-guest	972:981	arg1	interaction					983:993	the host-guest interaction	968:993	the host-guest interaction between cyclodextrin and azobenzene	968:1029	The self-healing behavior of this composited gel is based on the host-guest interaction between cyclodextrin and azobenzene.					
33483080	9	22	theme	tough	1239:1243	arg1	performance					1245:1255	highly stretchable and tough performance	1216:1255	highly stretchable and tough performance	1216:1255	Therefore, this gel can achieve self-healing ability while maintaining highly stretchable and tough performance.					
33483080	1	23	theme	Most	140:143	arg1	materials					169:177	Most structural self-healing materials	140:177	Most structural self-healing materials	140:177	Most structural self-healing materials were developed based on either reversible supramolecular interaction or dynamic covalent bonding.					
33483080	1	24	theme	covalent	259:266	arg1	bonding					268:274	dynamic covalent bonding	251:274	dynamic covalent bonding	251:274	Most structural self-healing materials were developed based on either reversible supramolecular interaction or dynamic covalent bonding.					
33483080	3	25	theme	stretchable	468:478	arg1	cyclodextrin					418:429	alginate-based cyclodextrin	403:429	alginate-based cyclodextrin	403:429	In this study, we fabricated the alginate-based cyclodextrin and polyacrylamide azobenzene highly stretchable and tough interpenetrating composite hydrogel with self-repairing behavior under light irradiation.					
33483080	1	26	theme	structural	145:154	arg1	materials					169:177	Most structural self-healing materials	140:177	Most structural self-healing materials	140:177	Most structural self-healing materials were developed based on either reversible supramolecular interaction or dynamic covalent bonding.					
33483080	8	27	theme	0.04	1105:1108	arg1	MPa					1110:1112	MPa	1110:1112	MPa	1110:1112	The recovery gel elongation at 48 h healing in the dark condition was is 0.04 MPa, with an elongation of 1140 %.					
33483080	1	28	theme	self-healing	156:167	arg1	materials					169:177	Most structural self-healing materials	140:177	Most structural self-healing materials	140:177	Most structural self-healing materials were developed based on either reversible supramolecular interaction or dynamic covalent bonding.					
33483080	8	29	theme	dark	1083:1086	arg1	condition					1088:1096	the dark condition	1079:1096	the dark condition	1079:1096	The recovery gel elongation at 48 h healing in the dark condition was is 0.04 MPa, with an elongation of 1140 %.					
33483080	8	30	theme	gel	1045:1047	arg1	elongation					1049:1058	The recovery gel elongation	1032:1058	The recovery gel elongation at 48 h healing in the dark condition	1032:1096	The recovery gel elongation at 48 h healing in the dark condition was is 0.04 MPa, with an elongation of 1140 %.					
33483080	0	31	theme	alginate-based	29:42	arg1	cyclodextrin/Azo-polyacrylamide					44:74	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide	0:74	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide	0:74	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide interpenetrating network hydrogel with self-healing properties.					
33483080	3	32	theme	self-repairing	531:544	arg1	behavior					546:553	self-repairing behavior	531:553	self-repairing behavior under light irradiation	531:577	In this study, we fabricated the alginate-based cyclodextrin and polyacrylamide azobenzene highly stretchable and tough interpenetrating composite hydrogel with self-repairing behavior under light irradiation.					
33483080	0	33	theme	tough	23:27	arg1	cyclodextrin/Azo-polyacrylamide					44:74	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide	0:74	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide	0:74	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide interpenetrating network hydrogel with self-healing properties.					
33483080	5	34	theme	structural	702:711	arg1	properties					741:750	The corresponding structural, thermal, and morphological properties	684:750	The corresponding structural, thermal, and morphological properties of hydrogels	684:763	The corresponding structural, thermal, and morphological properties of hydrogels were characterized.					
33483080	3	35	theme	composite	507:515	arg1	hydrogel					517:524	interpenetrating composite hydrogel	490:524	interpenetrating composite hydrogel with self-repairing behavior under light irradiation	490:577	In this study, we fabricated the alginate-based cyclodextrin and polyacrylamide azobenzene highly stretchable and tough interpenetrating composite hydrogel with self-repairing behavior under light irradiation.					
33483080	2	36	theme	good	288:291	arg1	idea					293:296	a good idea	286:296	a good idea to incorporate self-healing properties into high-performance materials	286:367	It seems a good idea to incorporate self-healing properties into high-performance materials.					
33483080	3	37	dep	cyclodextrin	418:429	arg1	the					399:401	the	399:401	the	399:401	In this study, we fabricated the alginate-based cyclodextrin and polyacrylamide azobenzene highly stretchable and tough interpenetrating composite hydrogel with self-repairing behavior under light irradiation.					
33483080	7	38	theme	composited	941:950	arg1	gel					952:954	this composited gel	936:954	this composited gel	936:954	The self-healing behavior of this composited gel is based on the host-guest interaction between cyclodextrin and azobenzene.					
33483080	8	39	with	MPa	1110:1112	arg1	elongation					1123:1132	an elongation	1120:1132	an elongation of 1140 %	1120:1142	The recovery gel elongation at 48 h healing in the dark condition was is 0.04 MPa, with an elongation of 1140 %.					
33483080	5	40	theme	thermal	714:720	arg1	properties					741:750	The corresponding structural, thermal, and morphological properties	684:750	The corresponding structural, thermal, and morphological properties of hydrogels	684:763	The corresponding structural, thermal, and morphological properties of hydrogels were characterized.					
33483080	7	41	theme	gel	952:954	arg1	behavior					924:931	The self-healing behavior	907:931	The self-healing behavior of this composited gel	907:954	The self-healing behavior of this composited gel is based on the host-guest interaction between cyclodextrin and azobenzene.					
33483080	6	42	theme	sol-gel	822:828	arg1	transformation					800:813	The reversible transformation	785:813	The reversible transformation of the sol-gel	785:828	The reversible transformation of the sol-gel can be achieved by the irradiation upon ultraviolet light and visible light.					
33483080	8	43	theme	h	1066:1066	arg1	healing					1068:1074	48 h healing	1063:1074	48 h healing in the dark condition	1063:1096	The recovery gel elongation at 48 h healing in the dark condition was is 0.04 MPa, with an elongation of 1140 %.					
33483080	3	44	theme	alginate-based	403:416	arg1	cyclodextrin					418:429	alginate-based cyclodextrin	403:429	alginate-based cyclodextrin	403:429	In this study, we fabricated the alginate-based cyclodextrin and polyacrylamide azobenzene highly stretchable and tough interpenetrating composite hydrogel with self-repairing behavior under light irradiation.					
33483080	8	45	theme	recovery	1036:1043	arg1	elongation					1049:1058	The recovery gel elongation	1032:1058	The recovery gel elongation at 48 h healing in the dark condition	1032:1096	The recovery gel elongation at 48 h healing in the dark condition was is 0.04 MPa, with an elongation of 1140 %.					
33483080	2	46	theme	self-healing	313:324	arg1	properties					326:335	self-healing properties	313:335	self-healing properties	313:335	It seems a good idea to incorporate self-healing properties into high-performance materials.					
33483080	9	47	theme	self-healing	1177:1188	arg1	ability					1190:1196	self-healing ability	1177:1196	self-healing ability	1177:1196	Therefore, this gel can achieve self-healing ability while maintaining highly stretchable and tough performance.					
33483080	7	48	theme	self-healing	911:922	arg1	behavior					924:931	The self-healing behavior	907:931	The self-healing behavior of this composited gel	907:954	The self-healing behavior of this composited gel is based on the host-guest interaction between cyclodextrin and azobenzene.					
33483080	0	49	theme	network	93:99	arg1	hydrogel					101:108	network hydrogel	93:108	network hydrogel	93:108	Highly stretchable and tough alginate-based cyclodextrin/Azo-polyacrylamide interpenetrating network hydrogel with self-healing properties.					
33483080	8	50	from	healing	1068:1074	arg1	elongation					1049:1058	The recovery gel elongation	1032:1058	The recovery gel elongation at 48 h healing in the dark condition	1032:1096	The recovery gel elongation at 48 h healing in the dark condition was is 0.04 MPa, with an elongation of 1140 %.					
33483080	8	50	from	healing	1068:1074	arg1	condition					1088:1096	the dark condition	1079:1096	the dark condition	1079:1096	The recovery gel elongation at 48 h healing in the dark condition was is 0.04 MPa, with an elongation of 1140 %.					
32222220	2	0	theme	disease	476:482	arg1	progression					484:494	disease progression	476:494	disease progression	476:494	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	4	1	dep	in	907:908	arg1	vitro					910:914	vitro	910:914	vitro	910:914	Despite these advantages, the cdECM has obvious limitations to mimic versatile ECMs precisely, suggesting the need for improved in vitro modeling to clarify the functions of native ECM.					
32222220	1	2	theme	dynamic	246:252	arg1	microenvironment					254:269	a dynamic microenvironment	244:269	cells a dynamic microenvironment	238:269	An extracellular matrix (ECM) has both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment.					
32222220	0	3	theme	native	89:94	arg1	ECM					96:98	native ECM	89:98	native ECM	89:98	Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM.					
32222220	3	4	theme	promising	706:714	arg1	tool					716:719	a promising tool	704:719	a promising tool for developing more physiological in vitro model systems	704:776	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	3	4	theme	promising	706:714	arg1	attention					691:699	much attention	686:699	much attention	686:699	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	4	5	theme	improved	898:905	arg1	modeling					916:923	improved in vitro modeling	898:923	improved in vitro modeling	898:923	Despite these advantages, the cdECM has obvious limitations to mimic versatile ECMs precisely, suggesting the need for improved in vitro modeling to clarify the functions of native ECM.					
32222220	3	6	theme	cell-derived	539:550	arg1	cdECM					572:576	cdECM	572:576	cdECM	572:576	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	3	6	theme	cell-derived	539:550	arg1	ECM					567:569	a cell-derived decellularized ECM	537:569	a cell-derived decellularized ECM (cdECM)	537:577	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	4	7	theme	native	953:958	arg1	ECM					960:962	native ECM	953:962	native ECM	953:962	Despite these advantages, the cdECM has obvious limitations to mimic versatile ECMs precisely, suggesting the need for improved in vitro modeling to clarify the functions of native ECM.					
32222220	3	8	theme	physiological	741:753	arg1	systems					770:776	more physiological in vitro model systems	736:776	more physiological in vitro model systems	736:776	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	6	9	theme	physiological	1260:1272	arg1	relevancy					1274:1282	physiological relevancy	1260:1282	physiological relevancy	1260:1282	In this chapter, we summarize the studies that re-engineered the cdECM to examine the features of native ECM in-depth and to increase physiological relevancy.					
32222220	0	10	link	cell-derived	14:25	arg1	matrix					41:46	cell-derived extracellular matrix	14:46	cell-derived extracellular matrix	14:46	Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM.					
32222220	0	10	link	cell-derived	14:25	arg1	approach					57:64	a new approach	51:64	a new approach to clarify the role of native ECM	51:98	Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM.					
32222220	3	11	theme	decellularized	552:565	arg1	cdECM					572:576	cdECM	572:576	cdECM	572:576	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	3	11	theme	decellularized	552:565	arg1	ECM					567:569	a cell-derived decellularized ECM	537:569	a cell-derived decellularized ECM (cdECM)	537:577	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	2	12	theme	developmental	506:518	arg1	processes					520:528	tissue developmental processes	499:528	tissue developmental processes	499:528	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	1	13	contain	has	131:133	arg1	matrix					118:123	An extracellular matrix	101:123	An extracellular matrix (ECM)	101:129	An extracellular matrix (ECM) has both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment.					
32222220	1	13	contain	has	131:133	arg2	characteristics					172:186	both biochemical and mechanophysical characteristics	135:186	both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment	135:269	An extracellular matrix (ECM) has both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment.					
32222220	1	13	contain	has	131:133	arg1	ECM					126:128	ECM	126:128	ECM	126:128	An extracellular matrix (ECM) has both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment.					
32222220	2	14	theme	reciprocal	279:288	arg1	interactions					290:301	reciprocal interactions	279:301	reciprocal interactions with ECM	279:310	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	1	15	theme	biochemical	140:150	arg1	characteristics					172:186	both biochemical and mechanophysical characteristics	135:186	both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment	135:269	An extracellular matrix (ECM) has both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment.					
32222220	0	16	theme	ECM	96:98	arg1	role					81:84	the role	77:84	the role of native ECM	77:98	Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM.					
32222220	3	17	link	cell-derived	539:550	arg1	cdECM					572:576	cdECM	572:576	cdECM	572:576	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	3	17	link	cell-derived	539:550	arg1	ECM					567:569	a cell-derived decellularized ECM	537:569	a cell-derived decellularized ECM (cdECM)	537:577	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	0	18	theme	cell-derived	14:25	arg1	matrix					41:46	cell-derived extracellular matrix	14:46	cell-derived extracellular matrix	14:46	Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM.					
32222220	0	18	theme	cell-derived	14:25	arg1	approach					57:64	a new approach	51:64	a new approach to clarify the role of native ECM	51:98	Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM.					
32222220	5	19	with	cdECM	1002:1006	arg1	systems					1072:1078	other systems	1066:1078	other systems	1066:1078	Recent studies propose to tailor the cdECM via biochemically, biomechanically, or incorporation with other systems as a new approach to address the limitations.					
32222220	3	20	theme	in	755:756	arg1	systems					770:776	more physiological in vitro model systems	736:776	more physiological in vitro model systems	736:776	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	1	21	theme	mechanophysical	156:170	arg1	characteristics					172:186	both biochemical and mechanophysical characteristics	135:186	both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment	135:269	An extracellular matrix (ECM) has both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment.					
32222220	6	22	theme	in-depth	1235:1242	arg1	ECM					1231:1233	native ECM in-depth	1224:1242	native ECM in-depth	1224:1242	In this chapter, we summarize the studies that re-engineered the cdECM to examine the features of native ECM in-depth and to increase physiological relevancy.					
32222220	2	23	theme	pivotal	426:432	arg1	role					434:437	a pivotal role	424:437	a pivotal role	424:437	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	6	24	theme	ECM	1231:1233	arg1	features					1212:1219	the features	1208:1219	the features of native ECM in-depth	1208:1242	In this chapter, we summarize the studies that re-engineered the cdECM to examine the features of native ECM in-depth and to increase physiological relevancy.					
32222220	0	25	theme	extracellular	27:39	arg1	matrix					41:46	cell-derived extracellular matrix	14:46	cell-derived extracellular matrix	14:46	Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM.					
32222220	0	25	theme	extracellular	27:39	arg1	approach					57:64	a new approach	51:64	a new approach to clarify the role of native ECM	51:98	Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM.					
32222220	5	26	theme	Recent	965:970	arg1	studies					972:978	Recent studies	965:978	Recent studies	965:978	Recent studies propose to tailor the cdECM via biochemically, biomechanically, or incorporation with other systems as a new approach to address the limitations.					
32222220	3	27	theme	model	764:768	arg1	systems					770:776	more physiological in vitro model systems	736:776	more physiological in vitro model systems	736:776	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	1	28	dep	cells	238:242	arg1	microenvironment					254:269	a dynamic microenvironment	244:269	cells a dynamic microenvironment	238:269	An extracellular matrix (ECM) has both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment.					
32222220	3	29	theme	vivo-like	588:596	arg1	heterogeneity					612:624	vivo-like compositional heterogeneity	588:624	vivo-like compositional heterogeneity	588:624	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	1	30	theme	multiple	202:209	arg1	components					211:220	multiple components	202:220	multiple components	202:220	An extracellular matrix (ECM) has both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment.					
32222220	2	31	theme	chemical	390:397	arg1	modification					399:410	chemical modification	390:410	chemical modification	390:410	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	5	32	theme	new	1085:1087	arg1	cdECM					1002:1006	the cdECM	998:1006	the cdECM via biochemically, biomechanically, or incorporation with other systems	998:1078	Recent studies propose to tailor the cdECM via biochemically, biomechanically, or incorporation with other systems as a new approach to address the limitations.					
32222220	5	32	theme	new	1085:1087	arg1	approach					1089:1096	a new approach	1083:1096	a new approach to address the limitations	1083:1123	Recent studies propose to tailor the cdECM via biochemically, biomechanically, or incorporation with other systems as a new approach to address the limitations.					
32222220	3	33	theme	compositional	598:610	arg1	heterogeneity					612:624	vivo-like compositional heterogeneity	588:624	vivo-like compositional heterogeneity	588:624	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	2	34	theme	tissue	499:504	arg1	processes					520:528	tissue developmental processes	499:528	tissue developmental processes	499:528	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	2	35	theme	biological	450:459	arg1	progression					484:494	disease progression	476:494	disease progression	476:494	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	2	35	theme	biological	450:459	arg1	processes					520:528	tissue developmental processes	499:528	tissue developmental processes	499:528	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	2	35	theme	biological	450:459	arg1	events					461:466	various biological events	442:466	various biological events such as disease progression or tissue developmental processes	442:528	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	4	36	theme	ECM	960:962	arg1	functions					940:948	the functions	936:948	the functions of native ECM	936:962	Despite these advantages, the cdECM has obvious limitations to mimic versatile ECMs precisely, suggesting the need for improved in vitro modeling to clarify the functions of native ECM.					
32222220	4	37	theme	versatile	848:856	arg1	ECMs					858:861	versatile ECMs	848:861	versatile ECMs	848:861	Despite these advantages, the cdECM has obvious limitations to mimic versatile ECMs precisely, suggesting the need for improved in vitro modeling to clarify the functions of native ECM.					
32222220	0	38	theme	new	53:55	arg1	matrix					41:46	cell-derived extracellular matrix	14:46	cell-derived extracellular matrix	14:46	Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM.					
32222220	0	38	theme	new	53:55	arg1	approach					57:64	a new approach	51:64	a new approach to clarify the role of native ECM	51:98	Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM.					
32222220	4	39	theme	in	907:908	arg1	modeling					916:923	improved in vitro modeling	898:923	improved in vitro modeling	898:923	Despite these advantages, the cdECM has obvious limitations to mimic versatile ECMs precisely, suggesting the need for improved in vitro modeling to clarify the functions of native ECM.					
32222220	2	40	theme	various	442:448	arg1	progression					484:494	disease progression	476:494	disease progression	476:494	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	2	40	theme	various	442:448	arg1	processes					520:528	tissue developmental processes	499:528	tissue developmental processes	499:528	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	2	40	theme	various	442:448	arg1	events					461:466	various biological events	442:466	various biological events such as disease progression or tissue developmental processes	442:528	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32222220	1	41	theme	extracellular	104:116	arg1	matrix					118:123	An extracellular matrix	101:123	An extracellular matrix (ECM)	101:129	An extracellular matrix (ECM) has both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment.					
32222220	1	41	theme	extracellular	104:116	arg1	ECM					126:128	ECM	126:128	ECM	126:128	An extracellular matrix (ECM) has both biochemical and mechanophysical characteristics obtained from multiple components, which provides cells a dynamic microenvironment.					
32222220	3	42	dep	in	755:756	arg1	vitro					758:762	vitro	758:762	vitro	758:762	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	3	43	theme	interconnected	630:643	arg1	architecture					656:667	interconnected fibrillary architecture	630:667	interconnected fibrillary architecture	630:667	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	6	44	theme	native	1224:1229	arg1	ECM					1231:1233	native ECM in-depth	1224:1242	native ECM in-depth	1224:1242	In this chapter, we summarize the studies that re-engineered the cdECM to examine the features of native ECM in-depth and to increase physiological relevancy.					
32222220	4	45	contain	has	815:817	arg1	cdECM					809:813	the cdECM	805:813	the cdECM	805:813	Despite these advantages, the cdECM has obvious limitations to mimic versatile ECMs precisely, suggesting the need for improved in vitro modeling to clarify the functions of native ECM.					
32222220	4	45	contain	has	815:817	arg2	limitations					827:837	obvious limitations	819:837	obvious limitations	819:837	Despite these advantages, the cdECM has obvious limitations to mimic versatile ECMs precisely, suggesting the need for improved in vitro modeling to clarify the functions of native ECM.					
32222220	5	46	theme	other	1066:1070	arg1	systems					1072:1078	other systems	1066:1078	other systems	1066:1078	Recent studies propose to tailor the cdECM via biochemically, biomechanically, or incorporation with other systems as a new approach to address the limitations.					
32222220	3	47	theme	fibrillary	645:654	arg1	architecture					656:667	interconnected fibrillary architecture	630:667	interconnected fibrillary architecture	630:667	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	6	48	dep	cdECM	1191:1195	arg1	examine					1200:1206	examine	1200:1206	to examine the features of native ECM in-depth	1197:1242	In this chapter, we summarize the studies that re-engineered the cdECM to examine the features of native ECM in-depth and to increase physiological relevancy.					
32222220	6	48	dep	cdECM	1191:1195	arg1	increase					1251:1258	increase	1251:1258	to increase physiological relevancy	1248:1282	In this chapter, we summarize the studies that re-engineered the cdECM to examine the features of native ECM in-depth and to increase physiological relevancy.					
32222220	3	49	theme	much	686:689	arg1	tool					716:719	a promising tool	704:719	a promising tool for developing more physiological in vitro model systems	704:776	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	3	49	theme	much	686:689	arg1	attention					691:699	much attention	686:699	much attention	686:699	Since a cell-derived decellularized ECM (cdECM) holds in vivo-like compositional heterogeneity and interconnected fibrillary architecture, it has received much attention as a promising tool for developing more physiological in vitro model systems.					
32222220	4	50	theme	obvious	819:825	arg1	limitations					827:837	obvious limitations	819:837	obvious limitations	819:837	Despite these advantages, the cdECM has obvious limitations to mimic versatile ECMs precisely, suggesting the need for improved in vitro modeling to clarify the functions of native ECM.					
32222220	2	51	with	interactions	290:301	arg1	ECM					308:310	ECM	308:310	ECM	308:310	During reciprocal interactions with ECM, the cells actively remodel the matrix, including synthesis, degradation, and chemical modification, which play a pivotal role in various biological events such as disease progression or tissue developmental processes.					
32987066	7	0	theme	alcoholic	1158:1166	arg1	extract					1168:1174	the alcoholic extract	1154:1174	the alcoholic extract provided that pH 8 or higher was employed	1154:1216	Intensive formation of AgNPs occurs only with the alcoholic extract provided that pH 8 or higher was employed.					
32987066	13	1	theme	cellulosic	2228:2237	arg1	fabrics					2239:2245	cellulosic fabrics	2228:2245	cellulosic fabrics	2228:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	13	2	with	products	2076:2083	arg1	performance					2106:2116	multifunctional performance	2090:2116	multifunctional performance	2090:2116	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	10	3	theme	fabrics	1628:1634	arg1	content					1550:1556	The nitrogen content	1537:1556	The nitrogen content	1537:1556	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	10	3	theme	fabrics	1628:1634	arg1	properties					1602:1611	the mechanical properties	1587:1611	the mechanical properties of the treated fabrics	1587:1634	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	10	3	theme	fabrics	1628:1634	arg1	properties					1572:1581	the physical properties	1559:1581	the physical properties	1559:1581	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	9	4	theme	spherical	1477:1485	arg1	shape					1487:1491	spherical shape	1477:1491	spherical shape with precise distribution of the particles	1477:1534	AgNPs were characterized by spherical shape with precise distribution of the particles.					
32987066	13	5	from	composite	2202:2210	arg1	fabrics					2239:2245	cellulosic fabrics	2228:2245	cellulosic fabrics	2228:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	13	5	from	composite	2202:2210	arg1	question					2215:2222	question	2215:2222	question	2215:2222	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	3	6	theme	stabilization	550:562	arg1	actions					564:570	the reducing and stabilization actions	533:570	the reducing and stabilization actions of Moringa extracts	533:590	The latter were prepared under the reducing and stabilization actions of Moringa extracts.					
32987066	5	7	theme	size	925:928	arg1	analyser					930:937	Particle size analyser	916:937	Particle size analyser	916:937	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	6	8	theme	different	1005:1013	arg1	attributes					1024:1033	different chemical attributes	1005:1033	different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6	1005:1105	Aqueous and alcoholic Moringa extracts exhibit different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6.					
32987066	11	9	theme	antimicrobial	1848:1860	arg1	activity					1862:1869	the high antimicrobial activity	1839:1869	the high antimicrobial activity imparted by current composites to the cellulosic fabrics	1839:1926	It was as well to emphasize the high antimicrobial activity imparted by current composites to the cellulosic fabrics.					
32987066	7	10	theme	Intensive	1108:1116	arg1	formation					1118:1126	Intensive formation	1108:1126	Intensive formation of AgNPs	1108:1135	Intensive formation of AgNPs occurs only with the alcoholic extract provided that pH 8 or higher was employed.					
32987066	3	11	theme	extracts	583:590	arg1	actions					564:570	the reducing and stabilization actions	533:570	the reducing and stabilization actions of Moringa extracts	533:590	The latter were prepared under the reducing and stabilization actions of Moringa extracts.					
32987066	8	12	theme	AgNPs	1240:1244	arg1	size					1232:1235	The particle size	1219:1235	The particle size of AgNPs	1219:1244	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	4	13	theme	variable	674:681	arg1	conditions					693:702	variable formation conditions	674:702	variable formation conditions of the required composites	674:729	These Precursors are mixed to form binary or tertiary mixture formulations under variable formation conditions of the required composites.					
32987066	10	14	theme	intercalation	1680:1692	arg1	interactions					1698:1709	interactions	1698:1709	interactions of Moringa aqueous and alcoholic extracts	1698:1751	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	10	14	theme	intercalation	1680:1692	arg1	magnitudes					1666:1675	the magnitudes	1662:1675	the magnitudes of intercalation	1662:1692	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	6	15	theme	alcoholic	970:978	arg1	extracts					988:995	Aqueous and alcoholic Moringa extracts	958:995	extracts	988:995	Aqueous and alcoholic Moringa extracts exhibit different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6.					
32987066	11	16	theme	current	1883:1889	arg1	composites					1891:1900	current composites	1883:1900	current composites to the cellulosic fabrics	1883:1926	It was as well to emphasize the high antimicrobial activity imparted by current composites to the cellulosic fabrics.					
32987066	1	17	from	cellulose	265:273	arg1	form					289:292	the fabric form	278:292	the fabric form	278:292	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	6	18	theme	Aqueous	958:964	arg1	extracts					988:995	Aqueous and alcoholic Moringa extracts	958:995	extracts	988:995	Aqueous and alcoholic Moringa extracts exhibit different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6.					
32987066	1	19	from	nature	206:211	arg1	precursors					190:199	precursors	190:199	precursors from nature	190:211	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	8	20	contain	containing	1418:1427	arg2	chitosan					1429:1436	chitosan	1429:1436	chitosan	1429:1436	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	8	20	contain	containing	1418:1427	arg1	those					1412:1416	those	1412:1416	those	1412:1416	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	8	20	contain	containing	1418:1427	arg2	AgNPs					1442:1446	AgNPs	1442:1446	AgNPs	1442:1446	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	4	21	theme	composites	720:729	arg1	conditions					693:702	variable formation conditions	674:702	variable formation conditions of the required composites	674:729	These Precursors are mixed to form binary or tertiary mixture formulations under variable formation conditions of the required composites.					
32987066	13	22	from	question	2215:2222	arg1	combination					2183:2193	chemical combination	2174:2193	chemical combination of the composite in question and cellulosic fabrics	2174:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	5	23	dep	composites	736:745	arg1	The					732:734	The	732:734	The	732:734	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	6	24	dep	attributes	1024:1033	arg1	fail					1059:1062	fail	1059:1062	fail to induce formation of AgNPs at up to pH 6	1059:1105	Aqueous and alcoholic Moringa extracts exhibit different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6.					
32987066	13	25	theme	attributes	2153:2162	arg1	impact					2130:2135	a direct impact	2121:2135	a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics	2121:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	13	25	theme	attributes	2153:2162	arg1	products					2076:2083	novel cellulosic products	2059:2083	novel cellulosic products with multifunctional performance	2059:2116	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	8	26	theme	larger	1386:1391	arg1	size					1402:1405	larger particle size	1386:1405	larger particle size	1386:1405	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	0	27	theme	other	97:101	arg1	precursors					111:120	other natural precursors	97:120	other natural precursors	97:120	Multifarious cellulosic through innovation of highly sustainable composites based on Moringa and other natural precursors.					
32987066	12	28	theme	treated	1994:2000	arg1	fabrics					2002:2008	the treated fabrics	1990:2008	the treated fabrics	1990:2008	Equal emphasis was placed on UPF and easy-care properties of the treated fabrics.					
32987066	10	29	theme	aqueous	1722:1728	arg1	interactions					1698:1709	interactions	1698:1709	interactions of Moringa aqueous and alcoholic extracts	1698:1751	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	10	29	theme	aqueous	1722:1728	arg1	magnitudes					1666:1675	the magnitudes	1662:1675	the magnitudes of intercalation	1662:1692	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	13	30	theme	direct	2123:2128	arg1	impact					2130:2135	a direct impact	2121:2135	a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics	2121:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	13	30	theme	direct	2123:2128	arg1	products					2076:2083	novel cellulosic products	2059:2083	novel cellulosic products with multifunctional performance	2059:2116	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	12	31	theme	UPF	1958:1960	arg1	properties					1976:1985	UPF and easy-care properties	1958:1985	UPF and easy-care properties of the treated fabrics	1958:2008	Equal emphasis was placed on UPF and easy-care properties of the treated fabrics.					
32987066	2	32	theme	silver	472:477	arg1	AgNPs					494:498	AgNPs	494:498	AgNPs	494:498	Precursors embrace Moringa oleifera leaves aqueous and alcoholic extracts, chitosan, clay known as Kaolin and, silver nanoparticles (AgNPs).					
32987066	2	32	theme	silver	472:477	arg1	nanoparticles					479:491	silver nanoparticles	472:491	silver nanoparticles (AgNPs)	472:499	Precursors embrace Moringa oleifera leaves aqueous and alcoholic extracts, chitosan, clay known as Kaolin and, silver nanoparticles (AgNPs).					
32987066	6	33	dep	pH 6	1102:1105	arg1	to					1099:1100	to	1099:1100	to	1099:1100	Aqueous and alcoholic Moringa extracts exhibit different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6.					
32987066	12	34	theme	easy-care	1966:1974	arg1	properties					1976:1985	UPF and easy-care properties	1958:1985	UPF and easy-care properties of the treated fabrics	1958:2008	Equal emphasis was placed on UPF and easy-care properties of the treated fabrics.					
32987066	13	35	from	fabrics	2239:2245	arg1	combination					2183:2193	chemical combination	2174:2193	chemical combination of the composite in question and cellulosic fabrics	2174:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	5	36	theme	state-of-the-art	867:882	arg1	facilities					884:893	state-of-the-art facilities	867:893	traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX	838:955	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	36	theme	state-of-the-art	867:882	arg1	EDX					953:955	EDX	953:955	EDX	953:955	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	36	theme	state-of-the-art	867:882	arg1	FT-IR					905:909	FT-IR	905:909	FT-IR	905:909	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	36	theme	state-of-the-art	867:882	arg1	analyser					930:937	Particle size analyser	916:937	Particle size analyser	916:937	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	36	theme	state-of-the-art	867:882	arg1	TEM					940:942	TEM	940:942	TEM	940:942	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	36	theme	state-of-the-art	867:882	arg1	UV					912:913	UV	912:913	UV	912:913	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	36	theme	state-of-the-art	867:882	arg1	SEM					945:947	SEM	945:947	SEM	945:947	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	10	37	theme	nitrogen	1541:1548	arg1	content					1550:1556	The nitrogen content	1537:1556	The nitrogen content	1537:1556	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	10	38	theme	alcoholic	1734:1742	arg1	extracts					1744:1751	Moringa aqueous and alcoholic extracts	1714:1751	extracts	1744:1751	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	0	39	theme	sustainable	53:63	arg1	composites					65:74	highly sustainable composites	46:74	highly sustainable composites based on Moringa and other natural precursors	46:120	Multifarious cellulosic through innovation of highly sustainable composites based on Moringa and other natural precursors.					
32987066	1	40	theme	performance	337:347	arg1	properties					349:358	multifunctional performance properties	321:358	multifunctional performance properties	321:358	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	10	41	theme	physical	1563:1570	arg1	properties					1572:1581	the physical properties	1559:1581	the physical properties	1559:1581	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	1	42	dep	synthesized	230:240	arg1	applied					247:253	applied	247:253	synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties	230:358	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	11	43	theme	cellulosic	1909:1918	arg1	fabrics					1920:1926	the cellulosic fabrics	1905:1926	the cellulosic fabrics	1905:1926	It was as well to emphasize the high antimicrobial activity imparted by current composites to the cellulosic fabrics.					
32987066	13	44	from	combination	2183:2193	arg1	fabrics					2239:2245	cellulosic fabrics	2228:2245	cellulosic fabrics	2228:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	13	44	from	combination	2183:2193	arg1	question					2215:2222	question	2215:2222	question	2215:2222	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	1	45	dep	formulated	218:227	arg1	synthesized					230:240	synthesized	230:240	synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties	230:358	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	1	46	theme	cotton	258:263	arg1	cellulose					265:273	cotton cellulose	258:273	cotton cellulose in the fabric form	258:292	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	5	47	theme	traditional	838:848	arg1	tools					850:854	traditional tools	838:854	traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX	838:955	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	47	theme	traditional	838:848	arg1	EDX					953:955	EDX	953:955	EDX	953:955	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	47	theme	traditional	838:848	arg1	FT-IR					905:909	FT-IR	905:909	FT-IR	905:909	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	47	theme	traditional	838:848	arg1	analyser					930:937	Particle size analyser	916:937	Particle size analyser	916:937	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	47	theme	traditional	838:848	arg1	TEM					940:942	TEM	940:942	TEM	940:942	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	47	theme	traditional	838:848	arg1	UV					912:913	UV	912:913	UV	912:913	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	5	47	theme	traditional	838:848	arg1	SEM					945:947	SEM	945:947	SEM	945:947	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	10	48	theme	mechanical	1591:1600	arg1	properties					1602:1611	the mechanical properties	1587:1611	the mechanical properties of the treated fabrics	1587:1634	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	13	49	theme	cellulosic	2065:2074	arg1	impact					2130:2135	a direct impact	2121:2135	a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics	2121:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	13	49	theme	cellulosic	2065:2074	arg1	products					2076:2083	novel cellulosic products	2059:2083	novel cellulosic products with multifunctional performance	2059:2116	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	10	50	theme	cellulosic	1792:1801	arg1	fabric					1803:1808	the cellulosic fabric	1788:1808	the cellulosic fabric	1788:1808	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	13	51	theme	current	2024:2030	arg1	research					2032:2039	current research	2024:2039	current research	2024:2039	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	1	52	theme	fabric	282:287	arg1	form					289:292	the fabric form	278:292	the fabric form	278:292	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	10	53	theme	treated	1620:1626	arg1	fabrics					1628:1634	the treated fabrics	1616:1634	the treated fabrics	1616:1634	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	7	54	theme	AgNPs	1131:1135	arg1	formation					1118:1126	Intensive formation	1108:1126	Intensive formation of AgNPs	1108:1135	Intensive formation of AgNPs occurs only with the alcoholic extract provided that pH 8 or higher was employed.					
32987066	5	55	theme	Particle	916:923	arg1	analyser					930:937	Particle size analyser	916:937	Particle size analyser	916:937	The composites and fabrics treated thereof were submitted to characterization, analysis and testing using traditional tools as well as state-of-the-art facilities including FT-IR, UV, Particle size analyser, TEM, SEM and EDX.					
32987066	4	56	theme	formation	683:691	arg1	conditions					693:702	variable formation conditions	674:702	variable formation conditions of the required composites	674:729	These Precursors are mixed to form binary or tertiary mixture formulations under variable formation conditions of the required composites.					
32987066	9	57	theme	precise	1498:1504	arg1	distribution					1506:1517	precise distribution	1498:1517	precise distribution of the particles	1498:1534	AgNPs were characterized by spherical shape with precise distribution of the particles.					
32987066	6	58	theme	chemical	1015:1022	arg1	attributes					1024:1033	different chemical attributes	1005:1033	different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6	1005:1105	Aqueous and alcoholic Moringa extracts exhibit different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6.					
32987066	3	59	theme	Moringa	575:581	arg1	extracts					583:590	Moringa extracts	575:590	Moringa extracts	575:590	The latter were prepared under the reducing and stabilization actions of Moringa extracts.					
32987066	8	60	theme	particle	1223:1230	arg1	size					1232:1235	The particle size	1219:1235	The particle size of AgNPs	1219:1244	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	8	61	theme	chemical	1288:1295	arg1	combination					1297:1307	chemical combination	1288:1307	chemical combination of Moringa extract	1288:1326	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	13	62	theme	composite	2202:2210	arg1	combination					2183:2193	chemical combination	2174:2193	chemical combination of the composite in question and cellulosic fabrics	2174:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	9	63	theme	particles	1526:1534	arg1	distribution					1506:1517	precise distribution	1498:1517	precise distribution of the particles	1498:1534	AgNPs were characterized by spherical shape with precise distribution of the particles.					
32987066	6	64	theme	Moringa	980:986	arg1	extracts					988:995	Aqueous and alcoholic Moringa extracts	958:995	extracts	988:995	Aqueous and alcoholic Moringa extracts exhibit different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6.					
32987066	9	65	with	shape	1487:1491	arg1	distribution					1506:1517	precise distribution	1498:1517	precise distribution of the particles	1498:1534	AgNPs were characterized by spherical shape with precise distribution of the particles.					
32987066	1	66	theme	Innovative	123:132	arg1	composites					134:143	Innovative composites	123:143	Innovative composites processed using sorted out and characterized precursors from nature	123:211	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	8	67	theme	extract	1320:1326	arg1	combination					1297:1307	chemical combination	1288:1307	chemical combination of Moringa extract	1288:1326	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	8	67	theme	extract	1320:1326	arg1	extract					1370:1376	chitosan Moringa aqueous or alcoholic extract	1332:1376	extract	1370:1376	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	13	68	theme	chemical	2174:2181	arg1	combination					2183:2193	chemical combination	2174:2193	chemical combination of the composite in question and cellulosic fabrics	2174:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	6	69	dep	meanwhile	1035:1043	arg1	both					1045:1048	both	1045:1048	both	1045:1048	Aqueous and alcoholic Moringa extracts exhibit different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6.					
32987066	4	70	theme	required	711:718	arg1	composites					720:729	the required composites	707:729	the required composites	707:729	These Precursors are mixed to form binary or tertiary mixture formulations under variable formation conditions of the required composites.					
32987066	13	71	theme	multifarious	2140:2151	arg1	attributes					2153:2162	multifarious attributes	2140:2162	multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics	2140:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	8	72	theme	alcoholic	1360:1368	arg1	extract					1370:1376	chitosan Moringa aqueous or alcoholic extract	1332:1376	extract	1370:1376	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	11	73	theme	high	1843:1846	arg1	activity					1862:1869	the high antimicrobial activity	1839:1869	the high antimicrobial activity imparted by current composites to the cellulosic fabrics	1839:1926	It was as well to emphasize the high antimicrobial activity imparted by current composites to the cellulosic fabrics.					
32987066	0	74	theme	natural	103:109	arg1	precursors					111:120	other natural precursors	97:120	other natural precursors	97:120	Multifarious cellulosic through innovation of highly sustainable composites based on Moringa and other natural precursors.					
32987066	12	75	theme	fabrics	2002:2008	arg1	properties					1976:1985	UPF and easy-care properties	1958:1985	UPF and easy-care properties of the treated fabrics	1958:2008	Equal emphasis was placed on UPF and easy-care properties of the treated fabrics.					
32987066	2	76	dep	oleifera	388:395	arg1	leaves					397:402	leaves	397:402	leaves aqueous and alcoholic extracts, chitosan, clay known as Kaolin and, silver nanoparticles (AgNPs)	397:499	Precursors embrace Moringa oleifera leaves aqueous and alcoholic extracts, chitosan, clay known as Kaolin and, silver nanoparticles (AgNPs).					
32987066	1	77	dep	using	155:159	arg1	sorted					161:166	sorted	161:166	sorted out	161:170	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	1	77	dep	using	155:159	arg1	characterized					176:188	characterized	176:188	characterized precursors from nature	176:211	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	4	78	theme	mixture	647:653	arg1	formulations					655:666	binary or tertiary mixture formulations	628:666	binary or tertiary mixture formulations	628:666	These Precursors are mixed to form binary or tertiary mixture formulations under variable formation conditions of the required composites.					
32987066	8	79	theme	particle	1393:1400	arg1	size					1402:1405	larger particle size	1386:1405	larger particle size	1386:1405	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	2	80	theme	alcoholic	416:424	arg1	extracts					426:433	aqueous and alcoholic extracts	404:433	extracts	426:433	Precursors embrace Moringa oleifera leaves aqueous and alcoholic extracts, chitosan, clay known as Kaolin and, silver nanoparticles (AgNPs).					
32987066	10	81	theme	extracts	1744:1751	arg1	interactions					1698:1709	interactions	1698:1709	interactions of Moringa aqueous and alcoholic extracts	1698:1751	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	10	81	theme	extracts	1744:1751	arg1	magnitudes					1666:1675	the magnitudes	1662:1675	the magnitudes of intercalation	1662:1692	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	8	82	theme	Moringa	1312:1318	arg1	extract					1320:1326	Moringa extract	1312:1326	Moringa extract	1312:1326	The particle size of AgNPs decreases by increasing the pH indicating chemical combination of Moringa extract and chitosan Moringa aqueous or alcoholic extract exhibit larger particle size than those containing chitosan and AgNPs.					
32987066	6	83	theme	AgNPs	1087:1091	arg1	formation					1074:1082	formation	1074:1082	formation of AgNPs	1074:1091	Aqueous and alcoholic Moringa extracts exhibit different chemical attributes meanwhile both extracts fail to induce formation of AgNPs at up to pH 6.					
32987066	0	84	theme	composites	65:74	arg1	innovation					32:41	innovation	32:41	innovation of highly sustainable composites based on Moringa and other natural precursors	32:120	Multifarious cellulosic through innovation of highly sustainable composites based on Moringa and other natural precursors.					
32987066	1	85	theme	multifunctional	321:335	arg1	properties					349:358	multifunctional performance properties	321:358	multifunctional performance properties	321:358	Innovative composites processed using sorted out and characterized precursors from nature were formulated, synthesized then applied to cotton cellulose in the fabric form to confer on the cellulose multifunctional performance properties.					
32987066	13	86	theme	multifunctional	2090:2104	arg1	performance					2106:2116	multifunctional performance	2090:2116	multifunctional performance	2090:2116	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	12	87	theme	Equal	1929:1933	arg1	emphasis					1935:1942	Equal emphasis	1929:1942	Equal emphasis	1929:1942	Equal emphasis was placed on UPF and easy-care properties of the treated fabrics.					
32987066	4	88	theme	binary	628:633	arg1	formulations					655:666	binary or tertiary mixture formulations	628:666	binary or tertiary mixture formulations	628:666	These Precursors are mixed to form binary or tertiary mixture formulations under variable formation conditions of the required composites.					
32987066	10	89	dep	fabric	1803:1808	arg1	composite					1773:1781	composite	1773:1781	composite	1773:1781	The nitrogen content, the physical properties and the mechanical properties of the treated fabrics were taken to demonstrate the magnitudes of intercalation and interactions of Moringa aqueous and alcoholic extracts individually and in composite with the cellulosic fabric.					
32987066	2	90	theme	aqueous	404:410	arg1	extracts					426:433	aqueous and alcoholic extracts	404:433	extracts	426:433	Precursors embrace Moringa oleifera leaves aqueous and alcoholic extracts, chitosan, clay known as Kaolin and, silver nanoparticles (AgNPs).					
32987066	4	91	theme	tertiary	638:645	arg1	formulations					655:666	binary or tertiary mixture formulations	628:666	binary or tertiary mixture formulations	628:666	These Precursors are mixed to form binary or tertiary mixture formulations under variable formation conditions of the required composites.					
32987066	13	92	theme	novel	2059:2063	arg1	impact					2130:2135	a direct impact	2121:2135	a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics	2121:2245	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	13	92	theme	novel	2059:2063	arg1	products					2076:2083	novel cellulosic products	2059:2083	novel cellulosic products with multifunctional performance	2059:2116	To this end, current research brings into focus novel cellulosic products with multifunctional performance as a direct impact of multifarious attributes caused by chemical combination of the composite in question and cellulosic fabrics.					
32987066	3	93	theme	reducing	537:544	arg1	actions					564:570	the reducing and stabilization actions	533:570	the reducing and stabilization actions of Moringa extracts	533:590	The latter were prepared under the reducing and stabilization actions of Moringa extracts.					
33652047	0	0	theme	biotechnological	94:109	arg1	applications					111:122	their potential biotechnological applications	78:122	their potential biotechnological applications	78:122	Characterization of exopolysaccharides produced by Weissella confusa XG-3 and their potential biotechnological applications.					
33652047	4	1	theme	nuclear	649:655	arg1	resonance					666:674	nuclear magnetic resonance	649:674	nuclear magnetic resonance (NMR) analyses	649:689	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	4	1	theme	nuclear	649:655	arg1	NMR					677:679	NMR	677:679	NMR	677:679	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	6	2	theme	good	962:965	arg1	activity					986:993	good radical scavenging activity	962:993	good radical scavenging activity	962:993	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	3	3	theme	force	484:488	arg1	microscopy					490:499	atomic force microscopy	477:499	atomic force microscopy (AFM)	477:505	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	3	3	theme	force	484:488	arg1	AFM					502:504	AFM	502:504	AFM	502:504	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	0	4	theme	potential	84:92	arg1	applications					111:122	their potential biotechnological applications	78:122	their potential biotechnological applications	78:122	Characterization of exopolysaccharides produced by Weissella confusa XG-3 and their potential biotechnological applications.					
33652047	4	5	theme	-linked	734:740	arg1	EPS					711:713	XG-3 EPS	706:713	XG-3 EPS	706:713	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	4	5	theme	-linked	734:740	arg1	dextran					742:748	a linear α-(1,6)-linked dextran	718:748	a linear α-(1,6)-linked dextran	718:748	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	7	6	theme	particle	1146:1153	arg1	size					1155:1158	particle size	1146:1158	particle size	1146:1158	The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration.					
33652047	7	7	theme	absolute	1105:1112	arg1	value					1114:1118	The absolute value	1101:1118	The absolute value of the zeta potential and particle size of XG-3 dextran	1101:1174	The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration.					
33652047	5	8	theme	chain	903:907	arg1	conformation					909:920	the random coiled chain conformation	885:920	the random coiled chain conformation of XG-3 dextran	885:936	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	5	9	theme	XG-3	925:928	arg1	dextran					930:936	XG-3 dextran	925:936	XG-3 dextran	925:936	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	7	10	theme	dextran	1214:1220	arg1	concentration					1222:1234	dextran concentration	1214:1234	dextran concentration	1214:1234	The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration.					
33652047	2	11	theme	GC	311:312	arg1	data					314:317	GC data	311:317	GC data	311:317	The monosaccharide composition of XG-3 EPS was determined to include glucose according to GC data, and its molecular weight was 3.19 × 106 Da, as determined by HPLC.					
33652047	3	12	from	presence	521:528	arg1	surfaces					569:576	irregular surfaces	559:576	irregular surfaces of XG-3 EPS	559:588	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	11	13	contain	has	1473:1475	arg1	additive					1541:1548	additive	1541:1548	additive	1541:1548	These findings indicate that XG-3 dextran has unique characteristics and can be potentially applied as a food additive and an antioxidant.					
33652047	11	13	contain	has	1473:1475	arg1	antioxidant					1557:1567	antioxidant	1557:1567	antioxidant	1557:1567	These findings indicate that XG-3 dextran has unique characteristics and can be potentially applied as a food additive and an antioxidant.					
33652047	11	13	contain	has	1473:1475	arg1	dextran					1465:1471	XG-3 dextran	1460:1471	XG-3 dextran	1460:1471	These findings indicate that XG-3 dextran has unique characteristics and can be potentially applied as a food additive and an antioxidant.					
33652047	11	13	contain	has	1473:1475	arg2	characteristics					1484:1498	unique characteristics	1477:1498	unique characteristics	1477:1498	These findings indicate that XG-3 dextran has unique characteristics and can be potentially applied as a food additive and an antioxidant.					
33652047	11	14	theme	XG-3	1460:1463	arg1	additive					1541:1548	additive	1541:1548	additive	1541:1548	These findings indicate that XG-3 dextran has unique characteristics and can be potentially applied as a food additive and an antioxidant.					
33652047	11	14	theme	XG-3	1460:1463	arg1	antioxidant					1557:1567	antioxidant	1557:1567	antioxidant	1557:1567	These findings indicate that XG-3 dextran has unique characteristics and can be potentially applied as a food additive and an antioxidant.					
33652047	11	14	theme	XG-3	1460:1463	arg1	dextran					1465:1471	XG-3 dextran	1460:1471	XG-3 dextran	1460:1471	These findings indicate that XG-3 dextran has unique characteristics and can be potentially applied as a food additive and an antioxidant.					
33652047	4	15	theme	XG-3	706:709	arg1	EPS					711:713	XG-3 EPS	706:713	XG-3 EPS	706:713	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	4	15	theme	XG-3	706:709	arg1	dextran					742:748	a linear α-(1,6)-linked dextran	718:748	a linear α-(1,6)-linked dextran	718:748	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	6	16	theme	thermal	1033:1039	arg1	stability					1041:1049	high thermal stability	1028:1049	high thermal stability	1028:1049	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	5	17	theme	diffraction	757:767	arg1	data					775:778	X-ray diffraction (XRD) data	751:778	X-ray diffraction (XRD) data	751:778	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	8	18	theme	XG-3	1273:1276	arg1	dextran					1278:1284	XG-3 dextran	1273:1284	XG-3 dextran	1273:1284	The water contact angle showed that XG-3 dextran had relatively high hydrophobicity in the presence of sucrose.					
33652047	1	19	theme	Weissella	178:186	arg1	XG-3					196:199	Weissella confusa XG-3	178:199	Weissella confusa XG-3	178:199	In this study, exopolysaccharides (EPSs) produced by Weissella confusa XG-3 were characterized.					
33652047	9	20	theme	XG-3	1349:1352	arg1	dextran					1354:1360	XG-3 dextran	1349:1360	XG-3 dextran	1349:1360	XG-3 dextran stimulated the growth of Lactobacillus spp.					
33652047	5	21	theme	assay	863:867	arg1	results					838:844	the results	834:844	the results of the Congo red assay	834:867	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	5	22	theme	random	889:894	arg1	conformation					909:920	the random coiled chain conformation	885:920	the random coiled chain conformation of XG-3 dextran	885:936	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	1	23	theme	confusa	188:194	arg1	XG-3					196:199	Weissella confusa XG-3	178:199	Weissella confusa XG-3	178:199	In this study, exopolysaccharides (EPSs) produced by Weissella confusa XG-3 were characterized.					
33652047	3	24	theme	Scanning	387:394	arg1	SEM					417:419	SEM	417:419	SEM	417:419	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	3	24	theme	Scanning	387:394	arg1	microscopy					405:414	Scanning electron microscopy	387:414	Scanning electron microscopy (SEM)	387:420	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	8	25	contain	had	1286:1288	arg2	hydrophobicity					1306:1319	relatively high hydrophobicity	1290:1319	relatively high hydrophobicity	1290:1319	The water contact angle showed that XG-3 dextran had relatively high hydrophobicity in the presence of sucrose.					
33652047	8	25	contain	had	1286:1288	arg1	dextran					1278:1284	XG-3 dextran	1273:1284	XG-3 dextran	1273:1284	The water contact angle showed that XG-3 dextran had relatively high hydrophobicity in the presence of sucrose.					
33652047	6	26	theme	reducing	999:1006	arg1	power					1008:1012	reducing power	999:1012	reducing power	999:1012	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	3	27	theme	lumps	539:543	arg1	presence					521:528	the presence	517:528	the presence of round lumps and chains on irregular surfaces of XG-3 EPS	517:588	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	7	28	theme	dextran	1168:1174	arg1	size					1155:1158	particle size	1146:1158	particle size	1146:1158	The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration.					
33652047	7	28	theme	dextran	1168:1174	arg1	potential					1132:1140	zeta potential	1127:1140	zeta potential	1127:1140	The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration.					
33652047	4	29	link	-linked	734:740	arg1	EPS					711:713	XG-3 EPS	706:713	XG-3 EPS	706:713	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	4	29	link	-linked	734:740	arg1	dextran					742:748	a linear α-(1,6)-linked dextran	718:748	a linear α-(1,6)-linked dextran	718:748	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	3	30	theme	electron	396:403	arg1	SEM					417:419	SEM	417:419	SEM	417:419	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	3	30	theme	electron	396:403	arg1	microscopy					405:414	Scanning electron microscopy	387:414	Scanning electron microscopy (SEM)	387:420	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	3	31	attach	presence	521:528	arg1	surfaces					569:576	irregular surfaces	559:576	irregular surfaces of XG-3 EPS	559:588	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	3	31	attach	presence	521:528	arg2	lumps					539:543	round lumps	533:543	round lumps	533:543	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	3	31	attach	presence	521:528	arg2	chains					549:554	chains	549:554	chains	549:554	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	6	32	theme	scavenging	975:984	arg1	activity					986:993	good radical scavenging activity	962:993	good radical scavenging activity	962:993	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	5	33	theme	red	859:861	arg1	assay					863:867	the Congo red assay	849:867	the Congo red assay	849:867	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	3	34	theme	irregular	559:567	arg1	surfaces					569:576	irregular surfaces	559:576	irregular surfaces of XG-3 EPS	559:588	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	5	35	theme	X-ray	751:755	arg1	XRD					770:772	XRD	770:772	XRD	770:772	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	5	35	theme	X-ray	751:755	arg1	diffraction					757:767	X-ray diffraction	751:767	X-ray diffraction (XRD) data	751:778	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	0	36	theme	exopolysaccharides	20:37	arg1	Characterization					0:15	Characterization	0:15	Characterization of exopolysaccharides	0:37	Characterization of exopolysaccharides produced by Weissella confusa XG-3 and their potential biotechnological applications.					
33652047	9	37	theme	spp	1401:1403	arg1	growth					1377:1382	the growth	1373:1382	the growth of Lactobacillus spp	1373:1403	XG-3 dextran stimulated the growth of Lactobacillus spp.					
33652047	6	38	theme	306.8 °C	1091:1098	arg1	temperature					1071:1081	a degradation temperature	1057:1081	a degradation temperature (Td) of 306.8 °C	1057:1098	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	6	38	theme	306.8 °C	1091:1098	arg1	Td					1084:1085	Td	1084:1085	Td	1084:1085	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	3	39	theme	round	533:537	arg1	lumps					539:543	round lumps	533:543	round lumps	533:543	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	3	40	theme	branched	453:460	arg1	structure					462:470	a smooth, porous, and branched structure	431:470	a smooth, porous, and branched structure	431:470	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	0	41	theme	Weissella	51:59	arg1	XG-3					69:72	Weissella confusa XG-3	51:72	Weissella confusa XG-3	51:72	Characterization of exopolysaccharides produced by Weissella confusa XG-3 and their potential biotechnological applications.					
33652047	4	42	theme	magnetic	657:664	arg1	resonance					666:674	nuclear magnetic resonance	649:674	nuclear magnetic resonance (NMR) analyses	649:689	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	4	42	theme	magnetic	657:664	arg1	NMR					677:679	NMR	677:679	NMR	677:679	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	7	43	theme	size	1155:1158	arg1	value					1114:1118	The absolute value	1101:1118	The absolute value of the zeta potential and particle size of XG-3 dextran	1101:1174	The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration.					
33652047	2	44	theme	EPS	260:262	arg1	composition					240:250	The monosaccharide composition	221:250	The monosaccharide composition of XG-3 EPS	221:262	The monosaccharide composition of XG-3 EPS was determined to include glucose according to GC data, and its molecular weight was 3.19 × 106 Da, as determined by HPLC.					
33652047	3	45	theme	porous	441:446	arg1	structure					462:470	a smooth, porous, and branched structure	431:470	a smooth, porous, and branched structure	431:470	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	3	46	theme	smooth	433:438	arg1	structure					462:470	a smooth, porous, and branched structure	431:470	a smooth, porous, and branched structure	431:470	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	7	47	dep	potential	1132:1140	arg1	the					1123:1125	the	1123:1125	the	1123:1125	The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration.					
33652047	2	48	theme	XG-3	255:258	arg1	EPS					260:262	XG-3 EPS	255:262	XG-3 EPS	255:262	The monosaccharide composition of XG-3 EPS was determined to include glucose according to GC data, and its molecular weight was 3.19 × 106 Da, as determined by HPLC.					
33652047	8	49	theme	water	1241:1245	arg1	angle					1255:1259	The water contact angle	1237:1259	The water contact angle	1237:1259	The water contact angle showed that XG-3 dextran had relatively high hydrophobicity in the presence of sucrose.					
33652047	3	50	theme	chains	549:554	arg1	presence					521:528	the presence	517:528	the presence of round lumps and chains on irregular surfaces of XG-3 EPS	517:588	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	6	51	theme	high	1028:1031	arg1	stability					1041:1049	high thermal stability	1028:1049	high thermal stability	1028:1049	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	4	52	theme	resonance	666:674	arg1	analyses					682:689	nuclear magnetic resonance (NMR) analyses	649:689	nuclear magnetic resonance (NMR) analyses	649:689	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	0	53	theme	confusa	61:67	arg1	XG-3					69:72	Weissella confusa XG-3	51:72	Weissella confusa XG-3	51:72	Characterization of exopolysaccharides produced by Weissella confusa XG-3 and their potential biotechnological applications.					
33652047	2	54	theme	monosaccharide	225:238	arg1	composition					240:250	The monosaccharide composition	221:250	The monosaccharide composition of XG-3 EPS	221:262	The monosaccharide composition of XG-3 EPS was determined to include glucose according to GC data, and its molecular weight was 3.19 × 106 Da, as determined by HPLC.					
33652047	11	55	theme	unique	1477:1482	arg1	characteristics					1484:1498	unique characteristics	1477:1498	unique characteristics	1477:1498	These findings indicate that XG-3 dextran has unique characteristics and can be potentially applied as a food additive and an antioxidant.					
33652047	9	56	theme	Lactobacillus	1387:1399	arg1	spp					1401:1403	Lactobacillus spp	1387:1403	Lactobacillus spp	1387:1403	XG-3 dextran stimulated the growth of Lactobacillus spp.					
33652047	3	57	theme	atomic	477:482	arg1	microscopy					490:499	atomic force microscopy	477:499	atomic force microscopy (AFM)	477:505	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	3	57	theme	atomic	477:482	arg1	AFM					502:504	AFM	502:504	AFM	502:504	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	8	58	theme	sucrose	1340:1346	arg1	presence					1328:1335	the presence	1324:1335	the presence of sucrose	1324:1346	The water contact angle showed that XG-3 dextran had relatively high hydrophobicity in the presence of sucrose.					
33652047	4	59	dep	Fourier	610:616	arg1	transform					618:626	transform	618:626	transform infrared (FT-IR)	618:643	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	5	60	theme	coiled	896:901	arg1	conformation					909:920	the random coiled chain conformation	885:920	the random coiled chain conformation of XG-3 dextran	885:936	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	3	61	theme	EPS	586:588	arg1	surfaces					569:576	irregular surfaces	559:576	irregular surfaces of XG-3 EPS	559:588	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	5	62	theme	dextran	930:936	arg1	conformation					909:920	the random coiled chain conformation	885:920	the random coiled chain conformation of XG-3 dextran	885:936	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	7	63	theme	XG-3	1163:1166	arg1	dextran					1168:1174	XG-3 dextran	1163:1174	XG-3 dextran	1163:1174	The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration.					
33652047	6	64	theme	degradation	1059:1069	arg1	temperature					1071:1081	a degradation temperature	1057:1081	a degradation temperature (Td) of 306.8 °C	1057:1098	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	6	64	theme	degradation	1059:1069	arg1	Td					1084:1085	Td	1084:1085	Td	1084:1085	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	6	65	theme	XG-3	939:942	arg1	dextran					944:950	XG-3 dextran	939:950	XG-3 dextran	939:950	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	6	66	contain	possessed	1018:1026	arg1	dextran					944:950	XG-3 dextran	939:950	XG-3 dextran	939:950	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	6	66	contain	possessed	1018:1026	arg2	stability					1041:1049	high thermal stability	1028:1049	high thermal stability	1028:1049	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	4	67	dep	infrared	628:635	arg1	FT-IR					638:642	FT-IR	638:642	FT-IR	638:642	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	2	68	theme	molecular	328:336	arg1	weight					338:343	its molecular weight	324:343	its molecular weight	324:343	The monosaccharide composition of XG-3 EPS was determined to include glucose according to GC data, and its molecular weight was 3.19 × 106 Da, as determined by HPLC.					
33652047	8	69	theme	contact	1247:1253	arg1	angle					1255:1259	The water contact angle	1237:1259	The water contact angle	1237:1259	The water contact angle showed that XG-3 dextran had relatively high hydrophobicity in the presence of sucrose.					
33652047	7	70	theme	zeta	1127:1130	arg1	potential					1132:1140	zeta potential	1127:1140	zeta potential	1127:1140	The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration.					
33652047	8	71	theme	high	1301:1304	arg1	hydrophobicity					1306:1319	relatively high hydrophobicity	1290:1319	relatively high hydrophobicity	1290:1319	The water contact angle showed that XG-3 dextran had relatively high hydrophobicity in the presence of sucrose.					
33652047	4	72	dep	transform	618:626	arg1	infrared					628:635	infrared	628:635	transform infrared (FT-IR)	618:643	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	6	73	theme	radical	967:973	arg1	activity					986:993	good radical scavenging activity	962:993	good radical scavenging activity	962:993	XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C.					
33652047	5	74	theme	noncrystalline	794:807	arg1	structure					819:827	the noncrystalline amorphous structure	790:827	the noncrystalline amorphous structure	790:827	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
33652047	4	75	theme	Fourier	610:616	arg1	results					595:601	The results	591:601	The results of the Fourier transform infrared (FT-IR)	591:643	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	4	75	theme	Fourier	610:616	arg1	analyses					682:689	nuclear magnetic resonance (NMR) analyses	649:689	nuclear magnetic resonance (NMR) analyses	649:689	The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran.					
33652047	7	76	theme	potential	1132:1140	arg1	value					1114:1118	The absolute value	1101:1118	The absolute value of the zeta potential and particle size of XG-3 dextran	1101:1174	The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration.					
33652047	3	77	theme	XG-3	581:584	arg1	EPS					586:588	XG-3 EPS	581:588	XG-3 EPS	581:588	Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS.					
33652047	5	78	theme	amorphous	809:817	arg1	structure					819:827	the noncrystalline amorphous structure	790:827	the noncrystalline amorphous structure	790:827	X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran.					
34402085	6	0	theme	HEP	1034:1036	arg1	rotation					1022:1029	The specific rotation	1009:1029	The specific rotation of HEP	1009:1036	The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃.					
34402085	3	1	theme	high-performance	504:519	arg1	chromatography					536:549	high-performance gel permeation chromatography	504:549	high-performance gel permeation chromatography	504:549	The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography.					
34402085	7	2	theme	DPPH	1365:1368	arg1	radicals					1408:1415	DPPH, hydroxyl, superoxide anion, and ABTS radicals	1365:1415	DPPH, hydroxyl, superoxide anion, and ABTS radicals	1365:1415	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	10	3	dep	APPLICATIONS	1725:1736	arg1	HEP					1763:1765	HEP	1763:1765	HEP	1763:1765	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	10	3	dep	APPLICATIONS	1725:1736	arg1	polysaccharide					1747:1760	A novel polysaccharide	1739:1760	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP)	1715:1766	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	8	4	theme	scavenging	1591:1600	arg1	ability					1602:1608	the scavenging ability	1587:1608	the scavenging ability of Vitamin C (Vc)	1587:1626	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	7	5	theme	anion	1392:1396	arg1	radicals					1408:1415	DPPH, hydroxyl, superoxide anion, and ABTS radicals	1365:1415	DPPH, hydroxyl, superoxide anion, and ABTS radicals	1365:1415	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	11	6	theme	anti-oxidation	2001:2014	arg1	products					2016:2023	developing new natural anti-oxidation products	1978:2023	developing new natural anti-oxidation products	1978:2023	This research proved the potential of HEP in anti-oxidation and provided the possibility of developing new natural anti-oxidation products.					
34402085	10	7	theme	potent	1774:1779	arg1	activity					1781:1788	a potent activity	1772:1788	a potent activity	1772:1788	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	6	8	theme	HEP	1202:1204	arg1	process					1191:1197	the pyrolysis process	1177:1197	the pyrolysis process of HEP	1177:1204	The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃.					
34402085	5	9	theme	containing	835:844	arg1	α-configuration					846:860	a pyranose containing α-configuration	824:860	a pyranose containing α-configuration	824:860	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	5	9	theme	containing	835:844	arg1	HEP					816:818	HEP	816:818	HEP	816:818	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	11	10	theme	new	1989:1991	arg1	products					2016:2023	developing new natural anti-oxidation products	1978:2023	developing new natural anti-oxidation products	1978:2023	This research proved the potential of HEP in anti-oxidation and provided the possibility of developing new natural anti-oxidation products.					
34402085	7	11	theme	certain	1337:1343	arg1	ability					1345:1351	a certain ability	1335:1351	a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals	1335:1415	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	10	12	contain	had	1768:1770	arg1	APPLICATIONS					1725:1736	PRACTICAL APPLICATIONS	1715:1736	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP)	1715:1766	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	10	12	contain	had	1768:1770	arg2	activity					1781:1788	a potent activity	1772:1788	a potent activity	1772:1788	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	10	13	theme	monosaccharide	1810:1823	arg1	composition					1825:1835	its monosaccharide composition	1806:1835	its monosaccharide composition	1806:1835	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	2	14	theme	thermal	302:308	arg1	stabilities					310:320	thermal stabilities	302:320	thermal stabilities	302:320	We researched its structure and thermal stabilities, and further studied its antioxidant activities in vitro.					
34402085	8	15	theme	C	1621:1621	arg1	ability					1602:1608	the scavenging ability	1587:1608	the scavenging ability of Vitamin C (Vc)	1587:1626	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	0	16	theme	antioxidant	114:124	arg1	activities					126:135	antioxidant activities	114:135	antioxidant activities	114:135	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	4	17	dep	fucose	613:618	arg1	galactose					620:628	galactose	620:628	fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01	613:723	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	4	17	dep	fucose	613:618	arg1	Fuc					661:663	Fuc	661:663	Fuc:Gal:Glu:Man:GlcA	661:680	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	4	17	dep	fucose	613:618	arg1	mannose					638:644	mannose	638:644	fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01	613:723	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	4	17	dep	fucose	613:618	arg1	acid					655:658	gluconic acid	646:658	fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01	613:723	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	4	17	dep	fucose	613:618	arg1	glucose					630:636	glucose	630:636	fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01	613:723	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	1	18	theme	Hericium	190:197	arg1	erinaceus					199:207	Hericium erinaceus	190:207	Hericium erinaceus	190:207	A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study.					
34402085	6	19	theme	pyrolysis	1181:1189	arg1	process					1191:1197	the pyrolysis process	1177:1197	the pyrolysis process of HEP	1177:1204	The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃.					
34402085	4	20	theme	gluconic	646:653	arg1	acid					655:658	gluconic acid	646:658	fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01	613:723	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	0	21	theme	in	137:138	arg1	Preparation					48:58	Preparation	48:58	Preparation	48:58	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	1	22	from	erinaceus	199:207	arg1	HEP					180:182	HEP	180:182	HEP	180:182	A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study.					
34402085	1	22	from	erinaceus	199:207	arg1	fraction					170:177	A novel polysaccharide fraction	147:177	A novel polysaccharide fraction (HEP) from Hericium erinaceus	147:207	A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study.					
34402085	6	23	theme	analysis	1148:1155	arg1	measurement					1115:1125	measurement	1115:1125	measurement of thermogravimetric analysis for HEP	1115:1163	The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃.					
34402085	8	24	theme	%	1542:1542	arg1	%					1550:1550	91.72% ± 0.17%	1537:1550	91.72% ± 0.17%	1537:1550	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	0	25	dep	in	137:138	arg1	vitro					140:144	vitro	140:144	vitro	140:144	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	1	26	theme	polysaccharide	155:168	arg1	HEP					180:182	HEP	180:182	HEP	180:182	A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study.					
34402085	1	26	theme	polysaccharide	155:168	arg1	fraction					170:177	A novel polysaccharide fraction	147:177	A novel polysaccharide fraction (HEP) from Hericium erinaceus	147:207	A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study.					
34402085	4	27	theme	1:2.87:0.09:0.12:0.01	703:723	arg1	ratio					694:698	a molar ratio	686:698	a molar ratio of 1:2.87:0.09:0.12:0.01	686:723	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	0	28	theme	thermal	89:95	arg1	stabilities					97:107	thermal stabilities	89:107	thermal stabilities	89:107	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	6	29	theme	specific	1013:1020	arg1	rotation					1022:1029	The specific rotation	1009:1029	The specific rotation of HEP	1009:1036	The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃.					
34402085	5	30	dep	infrared	760:767	arg1	Fourier-transformed					740:758	Fourier-transformed	740:758	Fourier-transformed	740:758	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	8	31	theme	strong	1446:1451	arg1	ability					1453:1459	a strong ability	1444:1459	a strong ability to scavenge DPPH-free radicals	1444:1490	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	3	32	theme	average	450:456	arg1	weight					468:473	an average molecular weight	447:473	an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography	447:549	The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography.					
34402085	10	33	theme	PRACTICAL	1715:1723	arg1	APPLICATIONS					1725:1736	PRACTICAL APPLICATIONS	1715:1736	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP)	1715:1766	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	5	34	dep	α-configuration	846:860	arg1	branched					966:973	branched	966:973	branched	966:973	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	0	35	theme	Hericium	28:35	arg1	erinaceus					37:45	Hericium erinaceus	28:45	Hericium erinaceus	28:45	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	9	36	theme	natural	1684:1690	arg1	component					1704:1712	a natural antioxidant component	1682:1712	a natural antioxidant component	1682:1712	Therefore, it was revealed that HEP might be used as a natural antioxidant component.					
34402085	9	36	theme	natural	1684:1690	arg1	HEP					1661:1663	HEP	1661:1663	HEP	1661:1663	Therefore, it was revealed that HEP might be used as a natural antioxidant component.					
34402085	8	37	theme	DPPH-free	1473:1481	arg1	radicals					1483:1490	DPPH-free radicals	1473:1490	DPPH-free radicals	1473:1490	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	4	38	theme	Ion	552:554	arg1	chromatography					556:569	Ion chromatography	552:569	Ion chromatography	552:569	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	10	39	theme	novel	1741:1745	arg1	HEP					1763:1765	HEP	1763:1765	HEP	1763:1765	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	10	39	theme	novel	1741:1745	arg1	polysaccharide					1747:1760	A novel polysaccharide	1739:1760	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP)	1715:1766	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	4	40	dep	Fuc	661:663	arg1	Glu					669:671	Glu	669:671	Fuc:Gal:Glu:Man:GlcA	661:680	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	4	40	dep	Fuc	661:663	arg1	Gal					665:667	Gal	665:667	Fuc:Gal:Glu:Man:GlcA	661:680	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	4	40	dep	Fuc	661:663	arg1	GlcA					677:680	GlcA	677:680	GlcA	677:680	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	4	40	dep	Fuc	661:663	arg1	Man					673:675	Man	673:675	Fuc:Gal:Glu:Man:GlcA	661:680	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	3	41	theme	gel	521:523	arg1	chromatography					536:549	high-performance gel permeation chromatography	504:549	high-performance gel permeation chromatography	504:549	The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography.					
34402085	7	42	theme	ABTS	1403:1406	arg1	radicals					1408:1415	DPPH, hydroxyl, superoxide anion, and ABTS radicals	1365:1415	DPPH, hydroxyl, superoxide anion, and ABTS radicals	1365:1415	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	8	43	theme	scavenging	1509:1518	arg1	rate					1520:1523	the highest scavenging rate	1497:1523	the highest scavenging rate	1497:1523	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	2	44	theme	antioxidant	347:357	arg1	activities					359:368	its antioxidant activities	343:368	its antioxidant activities	343:368	We researched its structure and thermal stabilities, and further studied its antioxidant activities in vitro.					
34402085	11	45	theme	developing	1978:1987	arg1	products					2016:2023	developing new natural anti-oxidation products	1978:2023	developing new natural anti-oxidation products	1978:2023	This research proved the potential of HEP in anti-oxidation and provided the possibility of developing new natural anti-oxidation products.					
34402085	1	46	theme	present	255:261	arg1	study					263:267	the present study	251:267	the present study	251:267	A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study.					
34402085	7	47	theme	superoxide	1381:1390	arg1	anion					1392:1396	superoxide anion	1381:1396	superoxide anion	1381:1396	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	8	48	contain	had	1440:1442	arg1	HEP					1436:1438	HEP	1436:1438	HEP	1436:1438	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	8	48	contain	had	1440:1442	arg2	ability					1453:1459	a strong ability	1444:1459	a strong ability to scavenge DPPH-free radicals	1444:1490	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	5	49	theme	pyranose	826:833	arg1	α-configuration					846:860	a pyranose containing α-configuration	824:860	a pyranose containing α-configuration	824:860	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	5	49	theme	pyranose	826:833	arg1	HEP					816:818	HEP	816:818	HEP	816:818	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	7	50	theme	hydroxyl	1371:1378	arg1	radicals					1408:1415	DPPH, hydroxyl, superoxide anion, and ABTS radicals	1365:1415	DPPH, hydroxyl, superoxide anion, and ABTS radicals	1365:1415	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	11	51	theme	products	2016:2023	arg1	possibility					1963:1973	the possibility	1959:1973	the possibility of developing new natural anti-oxidation products	1959:2023	This research proved the potential of HEP in anti-oxidation and provided the possibility of developing new natural anti-oxidation products.					
34402085	6	52	theme	thermal	1097:1103	arg1	stability					1105:1113	the thermal stability	1093:1113	the thermal stability	1093:1113	The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃.					
34402085	11	53	theme	natural	1993:1999	arg1	products					2016:2023	developing new natural anti-oxidation products	1978:2023	developing new natural anti-oxidation products	1978:2023	This research proved the potential of HEP in anti-oxidation and provided the possibility of developing new natural anti-oxidation products.					
34402085	8	54	theme	Vitamin	1613:1619	arg1	C					1621:1621	Vitamin C	1613:1621	Vitamin C (Vc)	1613:1626	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	8	54	theme	Vitamin	1613:1619	arg1	Vc					1624:1625	Vc	1624:1625	Vc	1624:1625	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	11	55	theme	HEP	1924:1926	arg1	potential					1911:1919	the potential	1907:1919	the potential of HEP in anti-oxidation	1907:1944	This research proved the potential of HEP in anti-oxidation and provided the possibility of developing new natural anti-oxidation products.					
34402085	6	56	theme	scanning	1068:1075	arg1	calorimetry					1077:1087	the differential scanning calorimetry	1051:1087	the differential scanning calorimetry	1051:1087	The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃.					
34402085	9	57	used	used	1674:1677	arg2	HEP					1661:1663	HEP	1661:1663	HEP	1661:1663	Therefore, it was revealed that HEP might be used as a natural antioxidant component.					
34402085	9	57	used	used	1674:1677	arg2	component					1704:1712	a natural antioxidant component	1682:1712	a natural antioxidant component	1682:1712	Therefore, it was revealed that HEP might be used as a natural antioxidant component.					
34402085	10	58	theme	sugar	1838:1842	arg1	residues					1844:1851	sugar residues	1838:1851	sugar residues	1838:1851	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	3	59	with	heteropolysaccharide	420:439	arg1	weight					468:473	an average molecular weight	447:473	an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography	447:549	The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography.					
34402085	7	60	theme	anti-oxidation	1288:1301	arg1	experiments					1303:1313	In vitro anti-oxidation experiments	1279:1313	In vitro anti-oxidation experiments	1279:1313	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	10	61	theme	physicochemical	1858:1872	arg1	properties					1874:1883	physicochemical properties	1858:1883	physicochemical properties	1858:1883	PRACTICAL APPLICATIONS: A novel polysaccharide (HEP) had a potent activity possibly due to its monosaccharide composition, sugar residues, and physicochemical properties.					
34402085	0	62	theme	structural	61:70	arg1	characteristics					72:86	structural characteristics	61:86	structural characteristics	61:86	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	7	63	theme	In	1279:1280	arg1	experiments					1303:1313	In vitro anti-oxidation experiments	1279:1313	In vitro anti-oxidation experiments	1279:1313	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	8	64	theme	highest	1501:1507	arg1	rate					1520:1523	the highest scavenging rate	1497:1523	the highest scavenging rate	1497:1523	It was found that HEP had a strong ability to scavenge DPPH-free radicals, and the highest scavenging rate could reach 91.72% ± 0.17%, which was basically equivalent to the scavenging ability of Vitamin C (Vc).					
34402085	1	65	theme	novel	149:153	arg1	HEP					180:182	HEP	180:182	HEP	180:182	A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study.					
34402085	1	65	theme	novel	149:153	arg1	fraction					170:177	A novel polysaccharide fraction	147:177	A novel polysaccharide fraction (HEP) from Hericium erinaceus	147:207	A novel polysaccharide fraction (HEP) from Hericium erinaceus was successively isolated and purified in the present study.					
34402085	11	66	from	potential	1911:1919	arg1	anti-oxidation					1931:1944	anti-oxidation	1931:1944	anti-oxidation	1931:1944	This research proved the potential of HEP in anti-oxidation and provided the possibility of developing new natural anti-oxidation products.					
34402085	4	67	from	fucose	613:618	arg1	ratio					694:698	a molar ratio	686:698	a molar ratio of 1:2.87:0.09:0.12:0.01	686:723	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	0	68	dep	polysaccharide	8:21	arg1	characteristics					72:86	structural characteristics	61:86	structural characteristics	61:86	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	0	68	dep	polysaccharide	8:21	arg1	stabilities					97:107	thermal stabilities	89:107	thermal stabilities	89:107	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	0	68	dep	polysaccharide	8:21	arg1	activities					126:135	antioxidant activities	114:135	antioxidant activities	114:135	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	0	68	dep	polysaccharide	8:21	arg1	Preparation					48:58	Preparation	48:58	Preparation	48:58	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	7	69	dep	In	1279:1280	arg1	vitro					1282:1286	vitro	1282:1286	vitro	1282:1286	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	5	70	theme	-α-D-Man-	937:945	arg1	-Glc					957:960	→3,6)-α-D-Man-(1→and→1,6)-Glc	932:960	→3,6)-α-D-Man-(1→and→1,6)-Glc	932:960	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	0	71	from	erinaceus	37:45	arg1	polysaccharide					8:21	A novel polysaccharide	0:21	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.	0:145	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
34402085	5	72	theme	NMR	773:775	arg1	spectroscopy					777:788	Fourier-transformed infrared and NMR spectroscopy	740:788	Fourier-transformed infrared and NMR spectroscopy	740:788	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	5	73	theme	main	915:918	arg1	chain					920:924	the main chain	911:924	the main chain	911:924	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	3	74	theme	acid	415:418	arg1	heteropolysaccharide					420:439	an acid heteropolysaccharide	412:439	an acid heteropolysaccharide	412:439	The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography.					
34402085	3	74	theme	acid	415:418	arg1	HEP					404:406	HEP	404:406	HEP	404:406	The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography.					
34402085	7	75	contain	had	1331:1333	arg2	ability					1345:1351	a certain ability	1335:1351	a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals	1335:1415	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	7	75	contain	had	1331:1333	arg1	HEP					1327:1329	HEP	1327:1329	HEP	1327:1329	In vitro anti-oxidation experiments showed that HEP had a certain ability to scavenge DPPH, hydroxyl, superoxide anion, and ABTS radicals.					
34402085	4	76	theme	molar	688:692	arg1	ratio					694:698	a molar ratio	686:698	a molar ratio of 1:2.87:0.09:0.12:0.01	686:723	Ion chromatography indicated that HEP was mainly composed of fucose:galactose:glucose:mannose:gluconic acid (Fuc:Gal:Glu:Man:GlcA) in a molar ratio of 1:2.87:0.09:0.12:0.01.					
34402085	3	77	theme	molecular	458:466	arg1	weight					468:473	an average molecular weight	447:473	an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography	447:549	The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography.					
34402085	6	78	theme	thermogravimetric	1130:1146	arg1	analysis					1148:1155	thermogravimetric analysis	1130:1155	thermogravimetric analysis for HEP	1130:1163	The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃.					
34402085	5	79	theme	infrared	760:767	arg1	spectroscopy					777:788	Fourier-transformed infrared and NMR spectroscopy	740:788	Fourier-transformed infrared and NMR spectroscopy	740:788	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	6	80	theme	differential	1055:1066	arg1	calorimetry					1077:1087	the differential scanning calorimetry	1051:1087	the differential scanning calorimetry	1051:1087	The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃.					
34402085	9	81	theme	antioxidant	1692:1702	arg1	component					1704:1712	a natural antioxidant component	1682:1712	a natural antioxidant component	1682:1712	Therefore, it was revealed that HEP might be used as a natural antioxidant component.					
34402085	9	81	theme	antioxidant	1692:1702	arg1	HEP					1661:1663	HEP	1661:1663	HEP	1661:1663	Therefore, it was revealed that HEP might be used as a natural antioxidant component.					
34402085	3	82	theme	19.7 kDa	492:499	arg1	weight					468:473	an average molecular weight	447:473	an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography	447:549	The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography.					
34402085	5	83	theme	1→and→1,6	947:955	arg1	-Glc					957:960	→3,6)-α-D-Man-(1→and→1,6)-Glc	932:960	→3,6)-α-D-Man-(1→and→1,6)-Glc	932:960	Additionally, Fourier-transformed infrared and NMR spectroscopy further demonstrated that HEP was a pyranose containing α-configuration, mainly consisting of α-1-4-Fuc and α-1-6-Gal as the main chain, with →3,6)-α-D-Man-(1→and→1,6)-Glc was branched, with α-D-GlcpA-(1 as T-terminal.					
34402085	6	84	theme	melting	1253:1259	arg1	point					1261:1265	its melting point	1249:1265	its melting point	1249:1265	The specific rotation of HEP was +55°; by the differential scanning calorimetry and the thermal stability measurement of thermogravimetric analysis for HEP showed that the pyrolysis process of HEP was mainly divided into two processes, and its melting point was 75.93℃.					
34402085	3	85	theme	permeation	525:534	arg1	chromatography					536:549	high-performance gel permeation chromatography	504:549	high-performance gel permeation chromatography	504:549	The results showed that HEP was an acid heteropolysaccharide, with an average molecular weight of approximately 19.7 kDa by high-performance gel permeation chromatography.					
34402085	0	86	theme	novel	2:6	arg1	polysaccharide					8:21	A novel polysaccharide	0:21	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.	0:145	A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro.					
32493173	6	0	theme	resin	731:735	arg1	composites					737:746	resin composites	731:746	resin composites	731:746	The mechanical properties of resin composites improved with cellulose content from 30 wt.					
32493173	2	1	theme	analytical	347:356	arg1	techniques					358:367	analytical techniques	347:367	analytical techniques	347:367	The microwave irradiation method was used for synthesis and the materials were characterized with analytical techniques.					
32493173	1	2	theme	dental	228:233	arg1	restorations					235:246	dental restorations	228:246	dental restorations	228:246	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	8	3	dep	%	802:802	arg1	matrix					819:824	the polymer matrix	807:824	the polymer matrix	807:824	% in the polymer matrix.					
32493173	5	4	theme	cell	656:659	arg1	viability					661:669	more than 90% cell viability	642:669	more than 90% cell viability	642:669	The Alamar blue biocompatibility assay showed more than 90% cell viability for the prepared cellulose/HA.					
32493173	5	5	theme	Alamar	600:605	arg1	assay					629:633	The Alamar blue biocompatibility assay	596:633	The Alamar blue biocompatibility assay	596:633	The Alamar blue biocompatibility assay showed more than 90% cell viability for the prepared cellulose/HA.					
32493173	4	6	theme	cellulose	578:586	arg1	fibers					588:593	cellulose fibers	578:593	cellulose fibers	578:593	FTIR, XRD, SEM/EDS analysis confirmed the successful synthesis of HA on cellulose fibers.					
32493173	4	7	dep	analysis	525:532	arg1	XRD					512:514	XRD	512:514	XRD	512:514	FTIR, XRD, SEM/EDS analysis confirmed the successful synthesis of HA on cellulose fibers.					
32493173	5	8	theme	blue	607:610	arg1	assay					629:633	The Alamar blue biocompatibility assay	596:633	The Alamar blue biocompatibility assay	596:633	The Alamar blue biocompatibility assay showed more than 90% cell viability for the prepared cellulose/HA.					
32493173	5	9	theme	biocompatibility	612:627	arg1	assay					629:633	The Alamar blue biocompatibility assay	596:633	The Alamar blue biocompatibility assay	596:633	The Alamar blue biocompatibility assay showed more than 90% cell viability for the prepared cellulose/HA.					
32493173	7	10	dep	%	791:791	arg1	50 wt					796:800	50 wt	796:800	50 wt	796:800	% to 50 wt.					
32493173	2	11	theme	irradiation	263:273	arg1	method					275:280	The microwave irradiation method	249:280	The microwave irradiation method	249:280	The microwave irradiation method was used for synthesis and the materials were characterized with analytical techniques.					
32493173	3	12	theme	universal	437:445	arg1	machine					455:461	a universal testing machine	435:461	a universal testing machine	435:461	The prepared dental resin composites were mechanically tested by a universal testing machine and electrodynamic fatigue testing system.					
32493173	4	13	theme	FTIR	506:509	arg1	analysis					525:532	FTIR, XRD, SEM/EDS analysis	506:532	analysis	525:532	FTIR, XRD, SEM/EDS analysis confirmed the successful synthesis of HA on cellulose fibers.					
32493173	4	13	theme	FTIR	506:509	arg1	SEM/EDS					517:523	SEM/EDS	517:523	SEM/EDS	517:523	FTIR, XRD, SEM/EDS analysis confirmed the successful synthesis of HA on cellulose fibers.					
32493173	9	14	theme	increasing	842:851	arg1	content					870:876	the cellulose/HA content	853:876	increasing the cellulose/HA content from 40% to 50%	842:892	Substantially, increasing the cellulose/HA content from 40% to 50% improved the mechanical properties.					
32493173	1	15	from	synthesis	133:141	arg1	fibers					179:184	cellulose fibers	169:184	cellulose fibers	169:184	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	2	16	theme	microwave	253:261	arg1	method					275:280	The microwave irradiation method	249:280	The microwave irradiation method	249:280	The microwave irradiation method was used for synthesis and the materials were characterized with analytical techniques.					
32493173	6	17	theme	mechanical	706:715	arg1	properties					717:726	The mechanical properties	702:726	The mechanical properties of resin composites	702:746	The mechanical properties of resin composites improved with cellulose content from 30 wt.					
32493173	1	18	theme	cellulose	169:177	arg1	fibers					179:184	cellulose fibers	169:184	cellulose fibers	169:184	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	3	19	theme	dental	383:388	arg1	composites					396:405	The prepared dental resin composites	370:405	The prepared dental resin composites	370:405	The prepared dental resin composites were mechanically tested by a universal testing machine and electrodynamic fatigue testing system.					
32493173	10	20	theme	resin	1107:1111	arg1	composites					1113:1122	dental resin composites	1100:1122	dental resin composites	1100:1122	The results suggested that HA could be successfully synthesized on cellulose fibers using microwave irradiation and contributed to improving the mechanical properties of dental resin composites.					
32493173	9	21	theme	50	890:891	arg1	%					885:885	%	885:885	%	885:885	Substantially, increasing the cellulose/HA content from 40% to 50% improved the mechanical properties.					
32493173	3	22	theme	resin	390:394	arg1	composites					396:405	The prepared dental resin composites	370:405	The prepared dental resin composites	370:405	The prepared dental resin composites were mechanically tested by a universal testing machine and electrodynamic fatigue testing system.					
32493173	9	23	theme	cellulose/HA	857:868	arg1	content					870:876	the cellulose/HA content	853:876	increasing the cellulose/HA content from 40% to 50%	842:892	Substantially, increasing the cellulose/HA content from 40% to 50% improved the mechanical properties.					
32493173	10	24	theme	mechanical	1075:1084	arg1	properties					1086:1095	the mechanical properties	1071:1095	the mechanical properties of dental resin composites	1071:1122	The results suggested that HA could be successfully synthesized on cellulose fibers using microwave irradiation and contributed to improving the mechanical properties of dental resin composites.					
32493173	5	25	dep	%	654:654	arg1	90					652:653	90	652:653	90	652:653	The Alamar blue biocompatibility assay showed more than 90% cell viability for the prepared cellulose/HA.					
32493173	1	26	theme	study	112:116	arg1	synthesis					133:141	an in-situ synthesis	122:141	an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers	122:184	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	1	26	theme	study	112:116	arg1	aim					100:102	The aim	96:102	The aim of this study	96:116	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	3	27	theme	electrodynamic	467:480	arg1	system					498:503	electrodynamic fatigue testing system	467:503	electrodynamic fatigue testing system	467:503	The prepared dental resin composites were mechanically tested by a universal testing machine and electrodynamic fatigue testing system.					
32493173	3	28	theme	prepared	374:381	arg1	composites					396:405	The prepared dental resin composites	370:405	The prepared dental resin composites	370:405	The prepared dental resin composites were mechanically tested by a universal testing machine and electrodynamic fatigue testing system.					
32493173	9	29	from	%	892:892	arg1	content					870:876	the cellulose/HA content	853:876	increasing the cellulose/HA content from 40% to 50%	842:892	Substantially, increasing the cellulose/HA content from 40% to 50% improved the mechanical properties.					
32493173	3	30	theme	fatigue	482:488	arg1	system					498:503	electrodynamic fatigue testing system	467:503	electrodynamic fatigue testing system	467:503	The prepared dental resin composites were mechanically tested by a universal testing machine and electrodynamic fatigue testing system.					
32493173	4	31	theme	HA	572:573	arg1	synthesis					559:567	the successful synthesis	544:567	the successful synthesis of HA on cellulose fibers	544:593	FTIR, XRD, SEM/EDS analysis confirmed the successful synthesis of HA on cellulose fibers.					
32493173	9	32	theme	40	883:884	arg1	%					885:885	%	885:885	%	885:885	Substantially, increasing the cellulose/HA content from 40% to 50% improved the mechanical properties.					
32493173	0	33	theme	cellulose/hydroxyapatite	34:57	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of cellulose/hydroxyapatite based dental restorative composites.					
32493173	0	33	theme	cellulose/hydroxyapatite	34:57	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of cellulose/hydroxyapatite based dental restorative composites.					
32493173	10	34	theme	microwave	1020:1028	arg1	irradiation					1030:1040	microwave irradiation	1020:1040	microwave irradiation	1020:1040	The results suggested that HA could be successfully synthesized on cellulose fibers using microwave irradiation and contributed to improving the mechanical properties of dental resin composites.					
32493173	0	35	theme	restorative	72:82	arg1	composites					84:93	dental restorative composites	65:93	dental restorative composites	65:93	Synthesis and characterization of cellulose/hydroxyapatite based dental restorative composites.					
32493173	9	36	dep	%	885:885	arg1	to					887:888	to	887:888	to	887:888	Substantially, increasing the cellulose/HA content from 40% to 50% improved the mechanical properties.					
32493173	10	37	theme	composites	1113:1122	arg1	properties					1086:1095	the mechanical properties	1071:1095	the mechanical properties of dental resin composites	1071:1122	The results suggested that HA could be successfully synthesized on cellulose fibers using microwave irradiation and contributed to improving the mechanical properties of dental resin composites.					
32493173	0	38	theme	dental	65:70	arg1	composites					84:93	dental restorative composites	65:93	dental restorative composites	65:93	Synthesis and characterization of cellulose/hydroxyapatite based dental restorative composites.					
32493173	4	39	theme	successful	548:557	arg1	synthesis					559:567	the successful synthesis	544:567	the successful synthesis of HA on cellulose fibers	544:593	FTIR, XRD, SEM/EDS analysis confirmed the successful synthesis of HA on cellulose fibers.					
32493173	5	40	theme	prepared	679:686	arg1	cellulose/HA					688:699	the prepared cellulose/HA	675:699	the prepared cellulose/HA	675:699	The Alamar blue biocompatibility assay showed more than 90% cell viability for the prepared cellulose/HA.					
32493173	1	41	theme	in-situ	125:131	arg1	synthesis					133:141	an in-situ synthesis	122:141	an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers	122:184	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	1	41	theme	in-situ	125:131	arg1	aim					100:102	The aim	96:102	The aim of this study	96:116	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	2	42	used	used	286:289	arg2	method					275:280	The microwave irradiation method	249:280	The microwave irradiation method	249:280	The microwave irradiation method was used for synthesis and the materials were characterized with analytical techniques.					
32493173	6	43	from	30 wt	785:789	arg1	content					772:778	cellulose content	762:778	cellulose content from 30 wt	762:789	The mechanical properties of resin composites improved with cellulose content from 30 wt.					
32493173	6	44	theme	cellulose	762:770	arg1	content					772:778	cellulose content	762:778	cellulose content from 30 wt	762:789	The mechanical properties of resin composites improved with cellulose content from 30 wt.					
32493173	3	45	theme	testing	490:496	arg1	system					498:503	electrodynamic fatigue testing system	467:503	electrodynamic fatigue testing system	467:503	The prepared dental resin composites were mechanically tested by a universal testing machine and electrodynamic fatigue testing system.					
32493173	8	46	theme	polymer	811:817	arg1	matrix					819:824	the polymer matrix	807:824	the polymer matrix	807:824	% in the polymer matrix.					
32493173	1	47	theme	new	202:204	arg1	agent					218:222	a new reinforcing agent	200:222	a new reinforcing agent for dental restorations	200:246	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	10	48	theme	cellulose	997:1005	arg1	fibers					1007:1012	cellulose fibers	997:1012	cellulose fibers using microwave irradiation	997:1040	The results suggested that HA could be successfully synthesized on cellulose fibers using microwave irradiation and contributed to improving the mechanical properties of dental resin composites.					
32493173	1	49	theme	reinforcing	206:216	arg1	agent					218:222	a new reinforcing agent	200:222	a new reinforcing agent for dental restorations	200:246	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	1	50	theme	hydroxyapatite	146:159	arg1	synthesis					133:141	an in-situ synthesis	122:141	an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers	122:184	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	1	50	theme	hydroxyapatite	146:159	arg1	aim					100:102	The aim	96:102	The aim of this study	96:116	The aim of this study was an in-situ synthesis of hydroxyapatite (HA) on cellulose fibers to be used as a new reinforcing agent for dental restorations.					
32493173	3	51	theme	testing	447:453	arg1	machine					455:461	a universal testing machine	435:461	a universal testing machine	435:461	The prepared dental resin composites were mechanically tested by a universal testing machine and electrodynamic fatigue testing system.					
32493173	9	52	theme	mechanical	907:916	arg1	properties					918:927	the mechanical properties	903:927	the mechanical properties	903:927	Substantially, increasing the cellulose/HA content from 40% to 50% improved the mechanical properties.					
32493173	10	53	theme	dental	1100:1105	arg1	composites					1113:1122	dental resin composites	1100:1122	dental resin composites	1100:1122	The results suggested that HA could be successfully synthesized on cellulose fibers using microwave irradiation and contributed to improving the mechanical properties of dental resin composites.					
32493173	4	54	from	synthesis	559:567	arg1	fibers					588:593	cellulose fibers	578:593	cellulose fibers	578:593	FTIR, XRD, SEM/EDS analysis confirmed the successful synthesis of HA on cellulose fibers.					
32493173	6	55	theme	composites	737:746	arg1	properties					717:726	The mechanical properties	702:726	The mechanical properties of resin composites	702:746	The mechanical properties of resin composites improved with cellulose content from 30 wt.					
32493173	5	56	dep	viability	661:669	arg1	%					654:654	%	654:654	%	654:654	The Alamar blue biocompatibility assay showed more than 90% cell viability for the prepared cellulose/HA.					
32147470	3	0	theme	nanoparticles	524:536	arg1	IgG-					490:493	IgG-	490:493	IgG-	490:493	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	3	0	theme	nanoparticles	524:536	arg1	trapping					512:519	IgM-mediated trapping	499:519	IgM-mediated trapping	499:519	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	1	1	theme	membranes	255:263	arg1	elements					224:231	the major structural elements	203:231	the major structural elements of mucus and basement membranes	203:263	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	3	2	theme	unique	585:590	arg1	features					601:608	unique chemical features	585:608	unique chemical features	585:608	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	8	3	theme	carboxyl	1366:1373	arg1	groups					1388:1393	carboxyl and hydroxyl groups	1366:1393	groups	1388:1393	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	3	4	from	IgG-	490:493	arg1	biogels					572:578	different polysaccharide-based biogels	541:578	different polysaccharide-based biogels with unique chemical features	541:608	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	8	5	from	matrix	1421:1426	arg1	bonding					1350:1356	hydrogen bonding	1341:1356	hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix	1341:1426	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	1	6	theme	viruses	174:180	arg1	crosslinking					158:169	Ab-mediated crosslinking	146:169	Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes	146:263	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	4	7	theme	anti-PEG	722:729	arg1	IgM					731:733	anti-PEG IgM	722:733	anti-PEG IgM but not anti-PEG IgG	722:754	In agarose, composed of alternating d-galactose and 3,6-anhydro-l-galactopyranose (i.e. hydroxyl groups only), anti-PEG IgM but not anti-PEG IgG trapped PEGylated nanoparticles.					
32147470	7	8	theme	IgG-mediated	1120:1131	arg1	trapping					1133:1140	IgG-mediated trapping	1120:1140	IgG-mediated trapping in alginate	1120:1152	IgG-mediated trapping in alginate was abrogated upon removal of IgG N-glycans, whereas IgM-mediated trapping was eliminated in agarose but not alginate upon desialylation.					
32147470	2	9	theme	biological	311:320	arg1	matrices					331:338	these biological hydrogel matrices	305:338	these biological hydrogel matrices to which Ab can bind	305:359	Nevertheless, the chemical moieties in these biological hydrogel matrices to which Ab can bind remain poorly understood.					
32147470	1	10	theme	nanoparticles	186:198	arg1	crosslinking					158:169	Ab-mediated crosslinking	146:169	Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes	146:263	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	12	11	theme	hydrogen	2155:2162	arg1	bonds					2164:2168	only hydroxyl/carboxyl hydrogen bonds	2132:2168	only hydroxyl/carboxyl hydrogen bonds	2132:2168	We discovered that only hydroxyl/carboxyl hydrogen bonds (and stronger) are sufficiently strong enough to facilitate antibody-mediated trapping; weaker hydroxyl/hydroxyl bonds or hydroxyl/amine bonds fail to adequately slow particles.					
32147470	12	12	theme	weaker	2258:2263	arg1	bonds					2283:2287	weaker hydroxyl/hydroxyl bonds	2258:2287	weaker hydroxyl/hydroxyl bonds	2258:2287	We discovered that only hydroxyl/carboxyl hydrogen bonds (and stronger) are sufficiently strong enough to facilitate antibody-mediated trapping; weaker hydroxyl/hydroxyl bonds or hydroxyl/amine bonds fail to adequately slow particles.					
32147470	10	13	theme	chemical	1772:1779	arg1	moieties					1781:1788	the chemical moieties	1768:1788	the chemical moieties on biogels that are essential for facilitating trapping in biogels	1768:1855	STATEMENT OF SIGNIFICANCE: Here, we interrogated the molecular mechanism of antibody-mediated trapping to address what are the chemical moieties on biogels that are essential for facilitating trapping in biogels.					
32147470	10	13	theme	chemical	1772:1779	arg1	what					1759:1762	what	1759:1762	what	1759:1762	STATEMENT OF SIGNIFICANCE: Here, we interrogated the molecular mechanism of antibody-mediated trapping to address what are the chemical moieties on biogels that are essential for facilitating trapping in biogels.					
32147470	5	14	theme	carboxyl	890:897	arg1	groups					899:904	both hydroxyl and carboxyl groups	872:904	groups	899:904	In alginate, comprised of homopolymeric blocks of mannuronate and guluronate (i.e. both hydroxyl and carboxyl groups), both IgG and IgM trapped PEGylated nanoparticles.					
32147470	1	15	theme	structural	213:222	arg1	elements					224:231	the major structural elements	203:231	the major structural elements of mucus and basement membranes	203:263	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	6	16	dep	comprised	981:989	arg1	groups					1054:1059	both hydroxyl and primary amine groups	1022:1059	groups	1054:1059	In contrast, chitosan, comprised primarily of glucosamine (i.e. both hydroxyl and primary amine groups), did not facilitate either IgG- or IgM-mediated trapping.					
32147470	6	16	dep	comprised	981:989	arg1	hydroxyl					1027:1034	both hydroxyl and primary amine groups	1022:1059	hydroxyl	1027:1034	In contrast, chitosan, comprised primarily of glucosamine (i.e. both hydroxyl and primary amine groups), did not facilitate either IgG- or IgM-mediated trapping.					
32147470	11	17	theme	hydroxyl	2029:2036	arg1	groups					2038:2043	hydroxyl groups	2029:2043	hydroxyl groups (agarose)	2029:2053	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	11	17	theme	hydroxyl	2029:2036	arg1	agarose					2046:2052	agarose	2046:2052	agarose	2046:2052	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	12	18	theme	only	2132:2135	arg1	bonds					2164:2168	only hydroxyl/carboxyl hydrogen bonds	2132:2168	only hydroxyl/carboxyl hydrogen bonds	2132:2168	We discovered that only hydroxyl/carboxyl hydrogen bonds (and stronger) are sufficiently strong enough to facilitate antibody-mediated trapping; weaker hydroxyl/hydroxyl bonds or hydroxyl/amine bonds fail to adequately slow particles.					
32147470	9	19	theme	biological	1620:1629	arg1	applications					1631:1642	various biomedical and biological applications	1597:1642	various biomedical and biological applications	1597:1642	Our work here offers a blueprint for designing de novo hydrogels that could harness Ab-matrix interactions for various biomedical and biological applications.					
32147470	13	20	theme	infectious	2545:2554	arg1	disease					2556:2562	infectious disease	2545:2562	infectious disease	2545:2562	Our findings presents the first blueprint for how to engineer de novo biogels that are capable of harnessing antibodies to immobilize foreign entities in the biogels, for applications ranging from infectious disease to contraception to purification processes.					
32147470	4	21	dep	d-galactose	647:657	arg1	groups					708:713	hydroxyl groups	699:713	hydroxyl groups only	699:718	In agarose, composed of alternating d-galactose and 3,6-anhydro-l-galactopyranose (i.e. hydroxyl groups only), anti-PEG IgM but not anti-PEG IgG trapped PEGylated nanoparticles.					
32147470	11	22	theme	amine	2056:2060	arg1	chitosan					2070:2077	chitosan	2070:2077	chitosan	2070:2077	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	11	22	theme	amine	2056:2060	arg1	groups					2062:2067	amine groups	2056:2067	amine groups (chitosan)	2056:2078	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	2	23	from	moieties	293:300	arg1	matrices					331:338	these biological hydrogel matrices	305:338	these biological hydrogel matrices to which Ab can bind	305:359	Nevertheless, the chemical moieties in these biological hydrogel matrices to which Ab can bind remain poorly understood.					
32147470	0	24	theme	sugar-sugar	66:76	arg1	bonds					87:91	sugar-sugar hydrogen bonds	66:91	sugar-sugar hydrogen bonds	66:91	Antibody-mediated trapping in biological hydrogels is governed by sugar-sugar hydrogen bonds.					
32147470	5	25	theme	mannuronate	839:849	arg1	blocks					829:834	homopolymeric blocks	815:834	homopolymeric blocks of mannuronate and guluronate (i.e. both hydroxyl and carboxyl groups)	815:905	In alginate, comprised of homopolymeric blocks of mannuronate and guluronate (i.e. both hydroxyl and carboxyl groups), both IgG and IgM trapped PEGylated nanoparticles.					
32147470	10	26	from	trapping	1837:1844	arg1	biogels					1849:1855	biogels	1849:1855	biogels	1849:1855	STATEMENT OF SIGNIFICANCE: Here, we interrogated the molecular mechanism of antibody-mediated trapping to address what are the chemical moieties on biogels that are essential for facilitating trapping in biogels.					
32147470	6	27	theme	amine	1048:1052	arg1	groups					1054:1059	both hydroxyl and primary amine groups	1022:1059	groups	1054:1059	In contrast, chitosan, comprised primarily of glucosamine (i.e. both hydroxyl and primary amine groups), did not facilitate either IgG- or IgM-mediated trapping.					
32147470	4	28	theme	anti-PEG	743:750	arg1	IgG					752:754	anti-PEG IgG	743:754	anti-PEG IgM but not anti-PEG IgG	722:754	In agarose, composed of alternating d-galactose and 3,6-anhydro-l-galactopyranose (i.e. hydroxyl groups only), anti-PEG IgM but not anti-PEG IgG trapped PEGylated nanoparticles.					
32147470	8	29	theme	hydroxyl	1379:1386	arg1	groups					1388:1393	carboxyl and hydroxyl groups	1366:1393	groups	1388:1393	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	2	30	theme	chemical	284:291	arg1	moieties					293:300	the chemical moieties	280:300	the chemical moieties in these biological hydrogel matrices to which Ab can bind	280:359	Nevertheless, the chemical moieties in these biological hydrogel matrices to which Ab can bind remain poorly understood.					
32147470	11	31	theme	unique	1984:1989	arg1	moieties					2019:2026	unique and highly defined chemical moieties	1984:2026	unique and highly defined chemical moieties	1984:2026	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	9	32	theme	various	1597:1603	arg1	applications					1631:1642	various biomedical and biological applications	1597:1642	various biomedical and biological applications	1597:1642	Our work here offers a blueprint for designing de novo hydrogels that could harness Ab-matrix interactions for various biomedical and biological applications.					
32147470	6	33	theme	IgM-mediated	1097:1108	arg1	trapping					1110:1117	IgM-mediated trapping	1097:1117	IgM-mediated trapping	1097:1117	In contrast, chitosan, comprised primarily of glucosamine (i.e. both hydroxyl and primary amine groups), did not facilitate either IgG- or IgM-mediated trapping.					
32147470	3	34	theme	different	541:549	arg1	biogels					572:578	different polysaccharide-based biogels	541:578	different polysaccharide-based biogels with unique chemical features	541:608	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	12	35	theme	hydroxyl/hydroxyl	2265:2281	arg1	bonds					2283:2287	weaker hydroxyl/hydroxyl bonds	2258:2287	weaker hydroxyl/hydroxyl bonds	2258:2287	We discovered that only hydroxyl/carboxyl hydrogen bonds (and stronger) are sufficiently strong enough to facilitate antibody-mediated trapping; weaker hydroxyl/hydroxyl bonds or hydroxyl/amine bonds fail to adequately slow particles.					
32147470	0	36	theme	Antibody-mediated	0:16	arg1	trapping					18:25	Antibody-mediated trapping	0:25	Antibody-mediated trapping in biological hydrogels	0:49	Antibody-mediated trapping in biological hydrogels is governed by sugar-sugar hydrogen bonds.					
32147470	8	37	from	bonding	1350:1356	arg1	matrix					1421:1426	matrix	1421:1426	matrix	1421:1426	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	8	37	from	bonding	1350:1356	arg1	Ab					1414:1415	Ab	1414:1415	Ab	1414:1415	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	13	38	theme	foreign	2482:2488	arg1	entities					2490:2497	foreign entities	2482:2497	foreign entities	2482:2497	Our findings presents the first blueprint for how to engineer de novo biogels that are capable of harnessing antibodies to immobilize foreign entities in the biogels, for applications ranging from infectious disease to contraception to purification processes.					
32147470	13	39	dep	de	2410:2411	arg1	novo					2413:2416	novo	2413:2416	novo	2413:2416	Our findings presents the first blueprint for how to engineer de novo biogels that are capable of harnessing antibodies to immobilize foreign entities in the biogels, for applications ranging from infectious disease to contraception to purification processes.					
32147470	11	40	theme	chemical	2010:2017	arg1	moieties					2019:2026	unique and highly defined chemical moieties	1984:2026	unique and highly defined chemical moieties	1984:2026	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	5	41	theme	hydroxyl	877:884	arg1	groups					899:904	both hydroxyl and carboxyl groups	872:904	groups	899:904	In alginate, comprised of homopolymeric blocks of mannuronate and guluronate (i.e. both hydroxyl and carboxyl groups), both IgG and IgM trapped PEGylated nanoparticles.					
32147470	12	42	theme	antibody-mediated	2230:2246	arg1	trapping					2248:2255	antibody-mediated trapping	2230:2255	antibody-mediated trapping	2230:2255	We discovered that only hydroxyl/carboxyl hydrogen bonds (and stronger) are sufficiently strong enough to facilitate antibody-mediated trapping; weaker hydroxyl/hydroxyl bonds or hydroxyl/amine bonds fail to adequately slow particles.					
32147470	10	43	theme	antibody-mediated	1721:1737	arg1	trapping					1739:1746	antibody-mediated trapping	1721:1746	antibody-mediated trapping	1721:1746	STATEMENT OF SIGNIFICANCE: Here, we interrogated the molecular mechanism of antibody-mediated trapping to address what are the chemical moieties on biogels that are essential for facilitating trapping in biogels.					
32147470	7	44	theme	N-glycans	1188:1196	arg1	removal					1173:1179	removal	1173:1179	removal of IgG N-glycans	1173:1196	IgG-mediated trapping in alginate was abrogated upon removal of IgG N-glycans, whereas IgM-mediated trapping was eliminated in agarose but not alginate upon desialylation.					
32147470	3	45	with	biogels	572:578	arg1	features					601:608	unique chemical features	585:608	unique chemical features	585:608	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	8	46	theme	Ab-mediated	1440:1450	arg1	trapping					1452:1459	Ab-mediated trapping	1440:1459	Ab-mediated trapping of pathogens in biogels	1440:1483	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	3	47	theme	IgM-mediated	499:510	arg1	trapping					512:519	IgM-mediated trapping	499:519	IgM-mediated trapping	499:519	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	5	48	theme	guluronate	855:864	arg1	blocks					829:834	homopolymeric blocks	815:834	homopolymeric blocks of mannuronate and guluronate (i.e. both hydroxyl and carboxyl groups)	815:905	In alginate, comprised of homopolymeric blocks of mannuronate and guluronate (i.e. both hydroxyl and carboxyl groups), both IgG and IgM trapped PEGylated nanoparticles.					
32147470	5	49	dep	groups	899:904	arg1	i.e.					867:870	i.e.	867:870	i.e.	867:870	In alginate, comprised of homopolymeric blocks of mannuronate and guluronate (i.e. both hydroxyl and carboxyl groups), both IgG and IgM trapped PEGylated nanoparticles.					
32147470	11	50	theme	different	1940:1948	arg1	biogels					1971:1977	different polysaccharide-based biogels	1940:1977	different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate)	1940:2110	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	1	51	theme	mucus	236:240	arg1	elements					224:231	the major structural elements	203:231	the major structural elements of mucus and basement membranes	203:263	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	1	52	theme	basement	246:253	arg1	membranes					255:263	basement membranes	246:263	basement membranes	246:263	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	8	53	from	glycans	1398:1404	arg1	matrix					1421:1426	matrix	1421:1426	matrix	1421:1426	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	8	53	from	glycans	1398:1404	arg1	Ab					1414:1415	Ab	1414:1415	Ab	1414:1415	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	11	54	theme	carboxyl	2085:2092	arg1	alginate					2102:2109	alginate	2102:2109	alginate	2102:2109	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	11	54	theme	carboxyl	2085:2092	arg1	groups					2094:2099	carboxyl groups	2085:2099	carboxyl groups (alginate)	2085:2110	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	5	55	theme	PEGylated	933:941	arg1	nanoparticles					943:955	PEGylated nanoparticles	933:955	PEGylated nanoparticles	933:955	In alginate, comprised of homopolymeric blocks of mannuronate and guluronate (i.e. both hydroxyl and carboxyl groups), both IgG and IgM trapped PEGylated nanoparticles.					
32147470	13	56	theme	purification	2584:2595	arg1	processes					2597:2605	purification processes	2584:2605	purification processes	2584:2605	Our findings presents the first blueprint for how to engineer de novo biogels that are capable of harnessing antibodies to immobilize foreign entities in the biogels, for applications ranging from infectious disease to contraception to purification processes.					
32147470	3	57	theme	polysaccharide-based	551:570	arg1	biogels					572:578	different polysaccharide-based biogels	541:578	different polysaccharide-based biogels with unique chemical features	541:608	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	10	58	from	moieties	1781:1788	arg1	biogels					1793:1799	biogels	1793:1799	biogels that are essential for facilitating trapping in biogels	1793:1855	STATEMENT OF SIGNIFICANCE: Here, we interrogated the molecular mechanism of antibody-mediated trapping to address what are the chemical moieties on biogels that are essential for facilitating trapping in biogels.					
32147470	10	59	theme	SIGNIFICANCE	1658:1669	arg1	STATEMENT					1645:1653	STATEMENT	1645:1653	STATEMENT OF SIGNIFICANCE	1645:1669	STATEMENT OF SIGNIFICANCE: Here, we interrogated the molecular mechanism of antibody-mediated trapping to address what are the chemical moieties on biogels that are essential for facilitating trapping in biogels.					
32147470	2	60	theme	hydrogel	322:329	arg1	matrices					331:338	these biological hydrogel matrices	305:338	these biological hydrogel matrices to which Ab can bind	305:359	Nevertheless, the chemical moieties in these biological hydrogel matrices to which Ab can bind remain poorly understood.					
32147470	13	61	theme	de	2410:2411	arg1	biogels					2418:2424	de novo biogels	2410:2424	de novo biogels that are capable of harnessing antibodies to immobilize foreign entities in the biogels	2410:2512	Our findings presents the first blueprint for how to engineer de novo biogels that are capable of harnessing antibodies to immobilize foreign entities in the biogels, for applications ranging from infectious disease to contraception to purification processes.					
32147470	3	62	from	trapping	512:519	arg1	biogels					572:578	different polysaccharide-based biogels	541:578	different polysaccharide-based biogels with unique chemical features	541:608	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	3	63	theme	chemical	592:599	arg1	features					601:608	unique chemical features	585:608	unique chemical features	585:608	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	13	64	theme	first	2374:2378	arg1	blueprint					2380:2388	the first blueprint	2370:2388	the first blueprint for how to engineer de novo biogels that are capable of harnessing antibodies to immobilize foreign entities in the biogels, for applications ranging from infectious disease to contraception to purification processes	2370:2605	Our findings presents the first blueprint for how to engineer de novo biogels that are capable of harnessing antibodies to immobilize foreign entities in the biogels, for applications ranging from infectious disease to contraception to purification processes.					
32147470	12	65	theme	hydroxyl/carboxyl	2137:2153	arg1	bonds					2164:2168	only hydroxyl/carboxyl hydrogen bonds	2132:2168	only hydroxyl/carboxyl hydrogen bonds	2132:2168	We discovered that only hydroxyl/carboxyl hydrogen bonds (and stronger) are sufficiently strong enough to facilitate antibody-mediated trapping; weaker hydroxyl/hydroxyl bonds or hydroxyl/amine bonds fail to adequately slow particles.					
32147470	8	66	from	trapping	1452:1459	arg1	biogels					1477:1483	biogels	1477:1483	biogels	1477:1483	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	1	67	theme	major	207:211	arg1	elements					224:231	the major structural elements	203:231	the major structural elements of mucus and basement membranes	203:263	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	4	68	theme	hydroxyl	699:706	arg1	groups					708:713	hydroxyl groups	699:713	hydroxyl groups only	699:718	In agarose, composed of alternating d-galactose and 3,6-anhydro-l-galactopyranose (i.e. hydroxyl groups only), anti-PEG IgM but not anti-PEG IgG trapped PEGylated nanoparticles.					
32147470	11	69	theme	IgG	1903:1905	arg1	potencies					1890:1898	the potencies	1886:1898	the potencies of IgG and IgM	1886:1913	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	11	70	with	biogels	1971:1977	arg1	chitosan					2070:2077	chitosan	2070:2077	chitosan	2070:2077	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	11	70	with	biogels	1971:1977	arg1	alginate					2102:2109	alginate	2102:2109	alginate	2102:2109	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	11	70	with	biogels	1971:1977	arg1	agarose					2046:2052	agarose	2046:2052	agarose	2046:2052	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	11	70	with	biogels	1971:1977	arg1	moieties					2019:2026	unique and highly defined chemical moieties	1984:2026	unique and highly defined chemical moieties	1984:2026	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	11	70	with	biogels	1971:1977	arg1	groups					2094:2099	carboxyl groups	2085:2099	carboxyl groups (alginate)	2085:2110	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	11	70	with	biogels	1971:1977	arg1	groups					2038:2043	hydroxyl groups	2029:2043	hydroxyl groups (agarose)	2029:2053	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	11	70	with	biogels	1971:1977	arg1	groups					2062:2067	amine groups	2056:2067	amine groups (chitosan)	2056:2078	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	1	71	theme	IgM	115:117	arg1	Ab					131:132	Ab	131:132	Ab	131:132	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	1	71	theme	IgM	115:117	arg1	antibodies					119:128	IgG and IgM antibodies	107:128	IgG and IgM antibodies (Ab)	107:133	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	9	72	theme	de	1533:1534	arg1	hydrogels					1541:1549	de novo hydrogels	1533:1549	de novo hydrogels that could harness Ab-matrix interactions for various biomedical and biological applications	1533:1642	Our work here offers a blueprint for designing de novo hydrogels that could harness Ab-matrix interactions for various biomedical and biological applications.					
32147470	9	73	dep	de	1533:1534	arg1	novo					1536:1539	novo	1536:1539	novo	1536:1539	Our work here offers a blueprint for designing de novo hydrogels that could harness Ab-matrix interactions for various biomedical and biological applications.					
32147470	4	74	theme	PEGylated	764:772	arg1	nanoparticles					774:786	PEGylated nanoparticles	764:786	PEGylated nanoparticles	764:786	In agarose, composed of alternating d-galactose and 3,6-anhydro-l-galactopyranose (i.e. hydroxyl groups only), anti-PEG IgM but not anti-PEG IgG trapped PEGylated nanoparticles.					
32147470	0	75	theme	hydrogen	78:85	arg1	bonds					87:91	sugar-sugar hydrogen bonds	66:91	sugar-sugar hydrogen bonds	66:91	Antibody-mediated trapping in biological hydrogels is governed by sugar-sugar hydrogen bonds.					
32147470	6	76	dep	hydroxyl	1027:1034	arg1	i.e.					1017:1020	i.e.	1017:1020	i.e.	1017:1020	In contrast, chitosan, comprised primarily of glucosamine (i.e. both hydroxyl and primary amine groups), did not facilitate either IgG- or IgM-mediated trapping.					
32147470	8	77	theme	hydrogen	1341:1348	arg1	bonding					1350:1356	hydrogen bonding	1341:1356	hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix	1341:1426	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	5	78	theme	homopolymeric	815:827	arg1	blocks					829:834	homopolymeric blocks	815:834	homopolymeric blocks of mannuronate and guluronate (i.e. both hydroxyl and carboxyl groups)	815:905	In alginate, comprised of homopolymeric blocks of mannuronate and guluronate (i.e. both hydroxyl and carboxyl groups), both IgG and IgM trapped PEGylated nanoparticles.					
32147470	1	79	theme	Ab-mediated	146:156	arg1	crosslinking					158:169	Ab-mediated crosslinking	146:169	Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes	146:263	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	6	80	theme	primary	1040:1046	arg1	groups					1054:1059	both hydroxyl and primary amine groups	1022:1059	groups	1054:1059	In contrast, chitosan, comprised primarily of glucosamine (i.e. both hydroxyl and primary amine groups), did not facilitate either IgG- or IgM-mediated trapping.					
32147470	0	81	from	trapping	18:25	arg1	hydrogels					41:49	biological hydrogels	30:49	biological hydrogels	30:49	Antibody-mediated trapping in biological hydrogels is governed by sugar-sugar hydrogen bonds.					
32147470	9	82	theme	Ab-matrix	1570:1578	arg1	interactions					1580:1591	Ab-matrix interactions	1570:1591	Ab-matrix interactions for various biomedical and biological applications	1570:1642	Our work here offers a blueprint for designing de novo hydrogels that could harness Ab-matrix interactions for various biomedical and biological applications.					
32147470	12	83	theme	slow	2332:2335	arg1	particles					2337:2345	adequately slow particles	2321:2345	adequately slow particles	2321:2345	We discovered that only hydroxyl/carboxyl hydrogen bonds (and stronger) are sufficiently strong enough to facilitate antibody-mediated trapping; weaker hydroxyl/hydroxyl bonds or hydroxyl/amine bonds fail to adequately slow particles.					
32147470	12	84	theme	hydroxyl/amine	2292:2305	arg1	bonds					2307:2311	hydroxyl/amine bonds	2292:2311	hydroxyl/amine bonds	2292:2311	We discovered that only hydroxyl/carboxyl hydrogen bonds (and stronger) are sufficiently strong enough to facilitate antibody-mediated trapping; weaker hydroxyl/hydroxyl bonds or hydroxyl/amine bonds fail to adequately slow particles.					
32147470	10	85	theme	molecular	1698:1706	arg1	mechanism					1708:1716	the molecular mechanism	1694:1716	the molecular mechanism of antibody-mediated trapping	1694:1746	STATEMENT OF SIGNIFICANCE: Here, we interrogated the molecular mechanism of antibody-mediated trapping to address what are the chemical moieties on biogels that are essential for facilitating trapping in biogels.					
32147470	7	86	theme	IgM-mediated	1207:1218	arg1	trapping					1220:1227	IgM-mediated trapping	1207:1227	IgM-mediated trapping	1207:1227	IgG-mediated trapping in alginate was abrogated upon removal of IgG N-glycans, whereas IgM-mediated trapping was eliminated in agarose but not alginate upon desialylation.					
32147470	9	87	theme	biomedical	1605:1614	arg1	applications					1631:1642	various biomedical and biological applications	1597:1642	various biomedical and biological applications	1597:1642	Our work here offers a blueprint for designing de novo hydrogels that could harness Ab-matrix interactions for various biomedical and biological applications.					
32147470	8	88	theme	glycans	1398:1404	arg1	bonding					1350:1356	hydrogen bonding	1341:1356	hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix	1341:1426	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	0	89	theme	biological	30:39	arg1	hydrogels					41:49	biological hydrogels	30:49	biological hydrogels	30:49	Antibody-mediated trapping in biological hydrogels is governed by sugar-sugar hydrogen bonds.					
32147470	1	90	theme	IgG	107:109	arg1	Ab					131:132	Ab	131:132	Ab	131:132	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	1	90	theme	IgG	107:109	arg1	antibodies					119:128	IgG and IgM antibodies	107:128	IgG and IgM antibodies (Ab)	107:133	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	7	91	from	trapping	1133:1140	arg1	alginate					1145:1152	alginate	1145:1152	alginate	1145:1152	IgG-mediated trapping in alginate was abrogated upon removal of IgG N-glycans, whereas IgM-mediated trapping was eliminated in agarose but not alginate upon desialylation.					
32147470	10	92	theme	trapping	1739:1746	arg1	mechanism					1708:1716	the molecular mechanism	1694:1716	the molecular mechanism of antibody-mediated trapping	1694:1746	STATEMENT OF SIGNIFICANCE: Here, we interrogated the molecular mechanism of antibody-mediated trapping to address what are the chemical moieties on biogels that are essential for facilitating trapping in biogels.					
32147470	7	93	theme	IgG	1184:1186	arg1	N-glycans					1188:1196	IgG N-glycans	1184:1196	IgG N-glycans	1184:1196	IgG-mediated trapping in alginate was abrogated upon removal of IgG N-glycans, whereas IgM-mediated trapping was eliminated in agarose but not alginate upon desialylation.					
32147470	4	94	dep	groups	708:713	arg1	i.e.					694:697	i.e.	694:697	i.e.	694:697	In agarose, composed of alternating d-galactose and 3,6-anhydro-l-galactopyranose (i.e. hydroxyl groups only), anti-PEG IgM but not anti-PEG IgG trapped PEGylated nanoparticles.					
32147470	3	95	theme	Ab-matrix	438:446	arg1	interactions					448:459	Ab-matrix interactions	438:459	Ab-matrix interactions	438:459	To gain insights into the chemistries that support Ab-matrix interactions, we systematically evaluated IgG- and IgM-mediated trapping of nanoparticles in different polysaccharide-based biogels with unique chemical features.					
32147470	11	96	theme	defined	2002:2008	arg1	moieties					2019:2026	unique and highly defined chemical moieties	1984:2026	unique and highly defined chemical moieties	1984:2026	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	10	97	dep	interrogated	1681:1692	arg1	STATEMENT					1645:1653	STATEMENT	1645:1653	STATEMENT OF SIGNIFICANCE	1645:1669	STATEMENT OF SIGNIFICANCE: Here, we interrogated the molecular mechanism of antibody-mediated trapping to address what are the chemical moieties on biogels that are essential for facilitating trapping in biogels.					
32147470	11	98	theme	IgM	1911:1913	arg1	potencies					1890:1898	the potencies	1886:1898	the potencies of IgG and IgM	1886:1913	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	1	99	from	N-glycans	94:102	arg1	Ab					131:132	Ab	131:132	Ab	131:132	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	1	99	from	N-glycans	94:102	arg1	antibodies					119:128	IgG and IgM antibodies	107:128	IgG and IgM antibodies (Ab)	107:133	N-glycans on IgG and IgM antibodies (Ab) facilitate Ab-mediated crosslinking of viruses and nanoparticles to the major structural elements of mucus and basement membranes.					
32147470	8	100	theme	pathogens	1464:1472	arg1	trapping					1452:1459	Ab-mediated trapping	1440:1459	Ab-mediated trapping of pathogens in biogels	1440:1483	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32147470	11	101	theme	polysaccharide-based	1950:1969	arg1	biogels					1971:1977	different polysaccharide-based biogels	1940:1977	different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate)	1940:2110	We systematically evaluated the potencies of IgG and IgM to trap nanoparticles in different polysaccharide-based biogels with unique and highly defined chemical moieties: hydroxyl groups (agarose), amine groups (chitosan), and carboxyl groups (alginate).					
32147470	8	102	from	Ab	1414:1415	arg1	bonding					1350:1356	hydrogen bonding	1341:1356	hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix	1341:1426	These results led us to propose a model in which hydrogen bonding between carboxyl and hydroxyl groups of glycans on both Ab and matrix facilitates Ab-mediated trapping of pathogens in biogels.					
32623828	7	0	theme	bacterial	1154:1162	arg1	culture					1164:1170	bacterial culture	1154:1170	bacterial culture	1154:1170	Furthermore, GP was completely ineffective for bacterial culture under highly acidic conditions (≤pH 1·0).					
32623828	3	1	theme	plates	644:649	arg1	diameter					575:582	the diameter	571:582	the diameter of Escherichia coli K12 colonies	571:615	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	3	1	theme	plates	644:649	arg1	stress					634:639	the breaking stress	621:639	the breaking stress	621:639	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	3	2	theme	breaking	625:632	arg1	stress					634:639	the breaking stress	621:639	the breaking stress	621:639	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	1	3	theme	suitable	247:254	arg1	methods					264:270	suitable culture methods	247:270	suitable culture methods	247:270	AIMS Several acidophilic bacteria have not been cultured, primarily owing to the lack of suitable culture methods under strong acidic conditions.					
32623828	1	4	theme	Several	163:169	arg1	bacteria					183:190	Several acidophilic bacteria	163:190	Several acidophilic bacteria	163:190	AIMS Several acidophilic bacteria have not been cultured, primarily owing to the lack of suitable culture methods under strong acidic conditions.					
32623828	6	5	theme	bacterial	1080:1088	arg1	≥72 h					1099:1103	≥72 h	1099:1103	≥72 h	1099:1103	CONCLUSIONS Assessment of the strength limits of AP and GP revealed that AP is not suitable for prolonged bacterial culture (≥72 h).					
32623828	6	5	theme	bacterial	1080:1088	arg1	culture					1090:1096	prolonged bacterial culture	1070:1096	prolonged bacterial culture (≥72 h)	1070:1104	CONCLUSIONS Assessment of the strength limits of AP and GP revealed that AP is not suitable for prolonged bacterial culture (≥72 h).					
32623828	3	6	theme	gelling	682:688	arg1	agents					690:695	gelling agents	682:695	gelling agents	682:695	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	3	7	theme	lower	508:512	arg1	limit					514:518	the lower limit	504:518	the lower limit of plate strength for bacterial isolation culture	504:568	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	3	8	theme	pH	721:722	arg1	conditions					724:733	pH conditions	721:733	pH conditions	721:733	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	0	9	theme	culture	118:124	arg1	Determination					0:12	Determination	0:12	Determination of available breaking stress of agar and gellan gum plate culture methods	0:86	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	0	9	theme	culture	118:124	arg1	duration					96:103	the duration	92:103	the duration of bacterial culture	92:124	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	3	10	theme	bacterial	542:550	arg1	culture					562:568	bacterial isolation culture	542:568	bacterial isolation culture	542:568	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	5	11	with	GP	906:907	arg1	medium					944:949	a high cationic concentration medium	914:949	a high cationic concentration medium	914:949	Medium composition slightly affected AP breaking stress, although GP with a high cationic concentration medium could not be prepared.					
32623828	3	12	theme	composition	705:715	arg1	concentrations					664:677	different concentrations	654:677	different concentrations of gelling agents, medium composition and pH conditions	654:733	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	0	13	theme	strong	132:137	arg1	conditions					146:155	strong acidic conditions	132:155	strong acidic conditions	132:155	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	3	14	theme	strength	529:536	arg1	limit					514:518	the lower limit	504:518	the lower limit of plate strength for bacterial isolation culture	504:568	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	8	15	theme	suitable	1374:1381	arg1	limit					1383:1387	the suitable limit	1370:1387	the suitable limit	1370:1387	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	2	16	theme	strong	448:453	arg1	conditions					462:471	strong acidic conditions	448:471	strong acidic conditions	448:471	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	0	17	theme	gellan	55:60	arg1	methods					80:86	agar and gellan gum plate culture methods	46:86	methods	80:86	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	2	18	theme	periods	434:440	arg1	strengths					352:360	the strengths	348:360	the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods	348:440	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	8	19	theme	culture	1398:1404	arg1	methods					1406:1412	plate culture methods	1392:1412	plate culture methods	1392:1412	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	0	20	theme	plate	66:70	arg1	methods					80:86	agar and gellan gum plate culture methods	46:86	methods	80:86	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	0	21	theme	methods	80:86	arg1	stress					36:41	available breaking stress	17:41	available breaking stress of agar and gellan gum plate culture methods	17:86	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	8	22	theme	evaluation	1268:1277	arg1	method					1279:1284	Our quantitative evaluation method	1251:1284	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress	1214:1309	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	6	23	theme	limits	1013:1018	arg1	Assessment					986:995	CONCLUSIONS Assessment	974:995	CONCLUSIONS Assessment of the strength limits of AP and GP	974:1031	CONCLUSIONS Assessment of the strength limits of AP and GP revealed that AP is not suitable for prolonged bacterial culture (≥72 h).					
32623828	8	24	theme	various	1435:1441	arg1	conditions					1443:1452	various conditions	1435:1452	various conditions	1435:1452	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	2	25	theme	agar	369:372	arg1	plates					374:379	the agar plates	365:379	the agar plates (AP)	365:384	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	2	25	theme	agar	369:372	arg1	AP					382:383	AP	382:383	AP	382:383	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	1	26	theme	culture	256:262	arg1	methods					264:270	suitable culture methods	247:270	suitable culture methods	247:270	AIMS Several acidophilic bacteria have not been cultured, primarily owing to the lack of suitable culture methods under strong acidic conditions.					
32623828	5	27	theme	breaking	880:887	arg1	stress					889:894	AP breaking stress	877:894	AP breaking stress	877:894	Medium composition slightly affected AP breaking stress, although GP with a high cationic concentration medium could not be prepared.					
32623828	2	28	theme	plates	401:406	arg1	strengths					352:360	the strengths	348:360	the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods	348:440	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	8	29	theme	breaking	1295:1302	arg1	stress					1304:1309	breaking stress	1295:1309	breaking stress	1295:1309	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	2	30	theme	gellan	390:395	arg1	GP					409:410	GP	409:410	GP	409:410	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	2	30	theme	gellan	390:395	arg1	plates					401:406	gellan gum plates	390:406	gellan gum plates (GP)	390:411	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	0	31	theme	available	17:25	arg1	stress					36:41	available breaking stress	17:41	available breaking stress of agar and gellan gum plate culture methods	17:86	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	2	32	theme	optimal	418:424	arg1	periods					434:440	optimal culture periods	418:440	optimal culture periods	418:440	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	0	33	theme	stress	36:41	arg1	Determination					0:12	Determination	0:12	Determination of available breaking stress of agar and gellan gum plate culture methods	0:86	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	0	33	theme	stress	36:41	arg1	duration					96:103	the duration	92:103	the duration of bacterial culture	92:124	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	1	34	theme	acidic	285:290	arg1	conditions					292:301	strong acidic conditions	278:301	strong acidic conditions	278:301	AIMS Several acidophilic bacteria have not been cultured, primarily owing to the lack of suitable culture methods under strong acidic conditions.					
32623828	4	35	theme	available	771:779	arg1	strength					781:788	available strength	771:788	available strength of AP and GP	771:801	The lower limit of available strength of AP and GP was 19·6 and 14·8 kPa, respectively.					
32623828	0	36	theme	agar	46:49	arg1	methods					80:86	agar and gellan gum plate culture methods	46:86	methods	80:86	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	8	37	dep	state	1360:1364	arg1	the					1356:1358	the	1356:1358	the	1356:1358	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	7	38	theme	≤pH	1204:1206	arg1	conditions					1192:1201	highly acidic conditions	1178:1201	highly acidic conditions (≤pH 1·0)	1178:1211	Furthermore, GP was completely ineffective for bacterial culture under highly acidic conditions (≤pH 1·0).					
32623828	7	38	theme	≤pH	1204:1206	arg1	1·0					1208:1210	≤pH 1·0	1204:1210	≤pH 1·0	1204:1210	Furthermore, GP was completely ineffective for bacterial culture under highly acidic conditions (≤pH 1·0).					
32623828	5	39	theme	cationic	921:928	arg1	medium					944:949	a high cationic concentration medium	914:949	a high cationic concentration medium	914:949	Medium composition slightly affected AP breaking stress, although GP with a high cationic concentration medium could not be prepared.					
32623828	3	40	theme	colonies	608:615	arg1	diameter					575:582	the diameter	571:582	the diameter of Escherichia coli K12 colonies	571:615	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	3	40	theme	colonies	608:615	arg1	stress					634:639	the breaking stress	621:639	the breaking stress	621:639	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	3	41	dep	Escherichia	587:597	arg1	coli					599:602	coli	599:602	coli	599:602	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	0	42	dep	Determination	0:12	arg1	conditions					146:155	strong acidic conditions	132:155	strong acidic conditions	132:155	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	6	43	theme	GP	1030:1031	arg1	limits					1013:1018	the strength limits	1000:1018	the strength limits of AP and GP	1000:1031	CONCLUSIONS Assessment of the strength limits of AP and GP revealed that AP is not suitable for prolonged bacterial culture (≥72 h).					
32623828	8	44	from	state	1360:1364	arg1	detail					1422:1427	more detail	1417:1427	more detail	1417:1427	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	4	45	theme	GP	800:801	arg1	strength					781:788	available strength	771:788	available strength of AP and GP	771:801	The lower limit of available strength of AP and GP was 19·6 and 14·8 kPa, respectively.					
32623828	6	46	theme	AP	1023:1024	arg1	limits					1013:1018	the strength limits	1000:1018	the strength limits of AP and GP	1000:1031	CONCLUSIONS Assessment of the strength limits of AP and GP revealed that AP is not suitable for prolonged bacterial culture (≥72 h).					
32623828	1	47	dep	AIMS	158:161	arg1	cultured					206:213	cultured	206:213	cultured	206:213	AIMS Several acidophilic bacteria have not been cultured, primarily owing to the lack of suitable culture methods under strong acidic conditions.					
32623828	4	48	theme	strength	781:788	arg1	limit					762:766	The lower limit	752:766	The lower limit of available strength of AP and GP	752:801	The lower limit of available strength of AP and GP was 19·6 and 14·8 kPa, respectively.					
32623828	4	48	theme	strength	781:788	arg1	19·6					807:810	19·6	807:810	19·6	807:810	The lower limit of available strength of AP and GP was 19·6 and 14·8 kPa, respectively.					
32623828	3	49	theme	different	654:662	arg1	concentrations					664:677	different concentrations	654:677	different concentrations of gelling agents, medium composition and pH conditions	654:733	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	1	50	theme	acidophilic	171:181	arg1	bacteria					183:190	Several acidophilic bacteria	163:190	Several acidophilic bacteria	163:190	AIMS Several acidophilic bacteria have not been cultured, primarily owing to the lack of suitable culture methods under strong acidic conditions.					
32623828	4	51	theme	AP	793:794	arg1	strength					781:788	available strength	771:788	available strength of AP and GP	771:801	The lower limit of available strength of AP and GP was 19·6 and 14·8 kPa, respectively.					
32623828	6	52	theme	prolonged	1070:1078	arg1	≥72 h					1099:1103	≥72 h	1099:1103	≥72 h	1099:1103	CONCLUSIONS Assessment of the strength limits of AP and GP revealed that AP is not suitable for prolonged bacterial culture (≥72 h).					
32623828	6	52	theme	prolonged	1070:1078	arg1	culture					1090:1096	prolonged bacterial culture	1070:1096	prolonged bacterial culture (≥72 h)	1070:1104	CONCLUSIONS Assessment of the strength limits of AP and GP revealed that AP is not suitable for prolonged bacterial culture (≥72 h).					
32623828	3	53	from	concentrations	664:677	arg1	diameter					575:582	the diameter	571:582	the diameter of Escherichia coli K12 colonies	571:615	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	3	53	from	concentrations	664:677	arg1	stress					634:639	the breaking stress	621:639	the breaking stress	621:639	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	3	54	theme	medium	698:703	arg1	composition					705:715	medium composition	698:715	medium composition	698:715	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	0	55	theme	bacterial	108:116	arg1	culture					118:124	bacterial culture	108:124	bacterial culture	108:124	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	3	56	theme	agents	690:695	arg1	concentrations					664:677	different concentrations	654:677	different concentrations of gelling agents, medium composition and pH conditions	654:733	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	3	57	theme	conditions	724:733	arg1	concentrations					664:677	different concentrations	654:677	different concentrations of gelling agents, medium composition and pH conditions	654:733	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	0	58	theme	acidic	139:144	arg1	conditions					146:155	strong acidic conditions	132:155	strong acidic conditions	132:155	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	3	59	theme	plate	523:527	arg1	strength					529:536	plate strength	523:536	plate strength	523:536	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	5	60	theme	Medium	840:845	arg1	composition					847:857	Medium composition	840:857	Medium composition	840:857	Medium composition slightly affected AP breaking stress, although GP with a high cationic concentration medium could not be prepared.					
32623828	8	61	dep	SIGNIFICANCE	1214:1225	arg1	method					1279:1284	Our quantitative evaluation method	1251:1284	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress	1214:1309	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	8	62	theme	plate	1392:1396	arg1	methods					1406:1412	plate culture methods	1392:1412	plate culture methods	1392:1412	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	0	63	theme	gum	62:64	arg1	methods					80:86	agar and gellan gum plate culture methods	46:86	methods	80:86	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	3	64	theme	isolation	552:560	arg1	culture					562:568	bacterial isolation culture	542:568	bacterial isolation culture	542:568	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	8	65	theme	methods	1406:1412	arg1	limit					1383:1387	the suitable limit	1370:1387	the suitable limit	1370:1387	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	8	65	theme	methods	1406:1412	arg1	state					1360:1364	state	1360:1364	state	1360:1364	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	0	66	theme	culture	72:78	arg1	methods					80:86	agar and gellan gum plate culture methods	46:86	methods	80:86	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	2	67	theme	culture	426:432	arg1	periods					434:440	optimal culture periods	418:440	optimal culture periods	418:440	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	8	68	theme	more	1417:1420	arg1	detail					1422:1427	more detail	1417:1427	more detail	1417:1427	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	8	69	theme	STUDY	1245:1249	arg1	IMPACT					1231:1236	IMPACT	1231:1236	IMPACT	1231:1236	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	8	69	theme	STUDY	1245:1249	arg1	SIGNIFICANCE					1214:1225	SIGNIFICANCE	1214:1225	SIGNIFICANCE	1214:1225	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	8	69	theme	STUDY	1245:1249	arg1	tool					1337:1340	a potentially valuable tool	1314:1340	a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions	1314:1452	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	8	70	from	limit	1383:1387	arg1	detail					1422:1427	more detail	1417:1427	more detail	1417:1427	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	2	71	theme	acidic	455:460	arg1	conditions					462:471	strong acidic conditions	448:471	strong acidic conditions	448:471	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	8	72	theme	quantitative	1255:1266	arg1	method					1279:1284	Our quantitative evaluation method	1251:1284	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress	1214:1309	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	2	73	theme	plates	374:379	arg1	strengths					352:360	the strengths	348:360	the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods	348:440	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	5	74	theme	AP	877:878	arg1	stress					889:894	AP breaking stress	877:894	AP breaking stress	877:894	Medium composition slightly affected AP breaking stress, although GP with a high cationic concentration medium could not be prepared.					
32623828	6	75	theme	strength	1004:1011	arg1	limits					1013:1018	the strength limits	1000:1018	the strength limits of AP and GP	1000:1031	CONCLUSIONS Assessment of the strength limits of AP and GP revealed that AP is not suitable for prolonged bacterial culture (≥72 h).					
32623828	0	76	theme	breaking	27:34	arg1	stress					36:41	available breaking stress	17:41	available breaking stress of agar and gellan gum plate culture methods	17:86	Determination of available breaking stress of agar and gellan gum plate culture methods and the duration of bacterial culture under strong acidic conditions.					
32623828	1	77	theme	methods	264:270	arg1	lack					239:242	the lack	235:242	the lack of suitable culture methods	235:270	AIMS Several acidophilic bacteria have not been cultured, primarily owing to the lack of suitable culture methods under strong acidic conditions.					
32623828	2	78	theme	gum	397:399	arg1	GP					409:410	GP	409:410	GP	409:410	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	2	78	theme	gum	397:399	arg1	plates					401:406	gellan gum plates	390:406	gellan gum plates (GP)	390:411	This study aimed to quantitatively evaluate the strengths of the agar plates (AP) and gellan gum plates (GP), and optimal culture periods under strong acidic conditions.					
32623828	3	79	theme	Escherichia	587:597	arg1	colonies					608:615	Escherichia coli K12 colonies	587:615	Escherichia coli K12 colonies	587:615	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	1	80	theme	strong	278:283	arg1	conditions					292:301	strong acidic conditions	278:301	strong acidic conditions	278:301	AIMS Several acidophilic bacteria have not been cultured, primarily owing to the lack of suitable culture methods under strong acidic conditions.					
32623828	6	81	theme	CONCLUSIONS	974:984	arg1	Assessment					986:995	CONCLUSIONS Assessment	974:995	CONCLUSIONS Assessment of the strength limits of AP and GP	974:1031	CONCLUSIONS Assessment of the strength limits of AP and GP revealed that AP is not suitable for prolonged bacterial culture (≥72 h).					
32623828	4	82	theme	lower	756:760	arg1	limit					762:766	The lower limit	752:766	The lower limit of available strength of AP and GP	752:801	The lower limit of available strength of AP and GP was 19·6 and 14·8 kPa, respectively.					
32623828	4	82	theme	lower	756:760	arg1	19·6					807:810	19·6	807:810	19·6	807:810	The lower limit of available strength of AP and GP was 19·6 and 14·8 kPa, respectively.					
32623828	5	83	theme	high	916:919	arg1	medium					944:949	a high cationic concentration medium	914:949	a high cationic concentration medium	914:949	Medium composition slightly affected AP breaking stress, although GP with a high cationic concentration medium could not be prepared.					
32623828	7	84	theme	acidic	1185:1190	arg1	conditions					1192:1201	highly acidic conditions	1178:1201	highly acidic conditions (≤pH 1·0)	1178:1211	Furthermore, GP was completely ineffective for bacterial culture under highly acidic conditions (≤pH 1·0).					
32623828	7	84	theme	acidic	1185:1190	arg1	1·0					1208:1210	≤pH 1·0	1204:1210	≤pH 1·0	1204:1210	Furthermore, GP was completely ineffective for bacterial culture under highly acidic conditions (≤pH 1·0).					
32623828	3	85	theme	K12	604:606	arg1	colonies					608:615	Escherichia coli K12 colonies	587:615	Escherichia coli K12 colonies	587:615	METHODS AND RESULTS To define the lower limit of plate strength for bacterial isolation culture, the diameter of Escherichia coli K12 colonies and the breaking stress of plates at different concentrations of gelling agents, medium composition and pH conditions were determined.					
32623828	8	86	theme	valuable	1328:1335	arg1	tool					1337:1340	a potentially valuable tool	1314:1340	a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions	1314:1452	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	8	86	theme	valuable	1328:1335	arg1	SIGNIFICANCE					1214:1225	SIGNIFICANCE	1214:1225	SIGNIFICANCE	1214:1225	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	8	86	theme	valuable	1328:1335	arg1	IMPACT					1231:1236	IMPACT	1231:1236	IMPACT	1231:1236	SIGNIFICANCE AND IMPACT OF THE STUDY Our quantitative evaluation method based on breaking stress is a potentially valuable tool to understand the state and the suitable limit of plate culture methods in more detail under various conditions.					
32623828	5	87	theme	concentration	930:942	arg1	medium					944:949	a high cationic concentration medium	914:949	a high cationic concentration medium	914:949	Medium composition slightly affected AP breaking stress, although GP with a high cationic concentration medium could not be prepared.					
34056088	0	0	theme	Spike	94:98	arg1	Analysis					61:68	Glycosylation Analysis	47:68	Glycosylation Analysis of the SARS-CoV-2 Viral Spike	47:98	Assessing Antigen Structural Integrity through Glycosylation Analysis of the SARS-CoV-2 Viral Spike.					
34056088	6	1	theme	monomeric	1100:1108	arg1	subunits					1110:1117	spike monomeric subunits	1094:1117	spike monomeric subunits	1094:1117	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	2	2	theme	main	349:352	arg1	target					354:359	the main target	345:359	the main target of the humoral immune response	345:390	Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.					
34056088	5	3	gly	glycoproteins	919:931	arg1	glycoproteins					919:931	engineered spike glycoproteins	902:931	engineered spike glycoproteins	902:931	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	1	4	theme	claimed	232:238	arg1	worldwide					256:264	claimed 2 776 175 lives worldwide	232:264	claimed 2 776 175 lives worldwide	232:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	0	5	theme	Viral	88:92	arg1	Spike					94:98	the SARS-CoV-2 Viral Spike	73:98	the SARS-CoV-2 Viral Spike	73:98	Assessing Antigen Structural Integrity through Glycosylation Analysis of the SARS-CoV-2 Viral Spike.					
34056088	1	6	theme	2 776 175	240:248	arg1	worldwide					256:264	claimed 2 776 175 lives worldwide	232:264	claimed 2 776 175 lives worldwide	232:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	3	7	theme	glycoprotein	564:575	arg1	production					577:586	spike glycoprotein production	558:586	spike glycoprotein production in vivo	558:594	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	1	8	theme	lives	250:254	arg1	worldwide					256:264	claimed 2 776 175 lives worldwide	232:264	claimed 2 776 175 lives worldwide	232:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	5	9	theme	wild-type	768:776	arg1	spikes					794:799	wild-type, non-stabilized spikes	768:799	wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence	768:868	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	6	10	theme	immune	1212:1217	arg1	response					1219:1226	the resulting immune response	1198:1226	the resulting immune response	1198:1226	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	3	11	contain	carry	406:410	arg2	glycans					412:418	glycans	412:418	glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization	412:517	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	3	11	contain	carry	406:410	arg1	spikes					399:404	Viral spikes	393:404	Viral spikes	393:404	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	2	12	theme	development	275:285	arg1	efforts					287:293	Vaccine development efforts	267:293	Vaccine development efforts	267:293	Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.					
34056088	6	13	theme	protein	1026:1032	arg1	glycosylation					1034:1046	protein glycosylation	1026:1046	protein glycosylation	1026:1046	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	6	14	theme	wild-type	1142:1150	arg1	spike					1152:1156	the non-stabilized wild-type spike	1123:1156	the non-stabilized wild-type spike tested	1123:1163	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	2	15	theme	Vaccine	267:273	arg1	efforts					287:293	Vaccine development efforts	267:293	Vaccine development efforts	267:293	Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.					
34056088	2	16	theme	humoral	368:374	arg1	response					383:390	the humoral immune response	364:390	the humoral immune response	364:390	Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.					
34056088	2	17	theme	response	383:390	arg1	target					354:359	the main target	345:359	the main target of the humoral immune response	345:390	Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.					
34056088	5	18	theme	CMV	833:835	arg1	promoter					837:844	a CMV promoter	831:844	a CMV promoter	831:844	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	6	19	theme	secretion	1052:1060	arg1	patterns					1062:1069	different protein glycosylation and secretion patterns	1016:1069	different protein glycosylation and secretion patterns	1016:1069	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	6	19	theme	secretion	1052:1060	arg1	shedding					1082:1089	shedding	1082:1089	shedding of spike monomeric subunits for the non-stabilized wild-type spike tested	1082:1163	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	4	20	theme	immunogen	608:616	arg1	integrity					618:626	immunogen integrity	608:626	immunogen integrity	608:626	Therefore, immunogen integrity is important for glycoprotein-based vaccine candidates.					
34056088	1	21	theme	COVID-19	182:189	arg1	pandemic					191:198	the COVID-19 pandemic	178:198	the COVID-19 pandemic	178:198	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	0	22	theme	Antigen	10:16	arg1	Integrity					29:37	Antigen Structural Integrity	10:37	Antigen Structural Integrity	10:37	Assessing Antigen Structural Integrity through Glycosylation Analysis of the SARS-CoV-2 Viral Spike.					
34056088	3	23	theme	antigen	524:530	arg1	efficacy					532:539	antigen efficacy	524:539	antigen efficacy	524:539	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	2	24	theme	viral	308:312	arg1	glycoprotein					329:340	the viral trimeric spike glycoprotein	304:340	the viral trimeric spike glycoprotein as the main target of the humoral immune response	304:390	Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.					
34056088	1	25	theme	pandemic	191:198	arg1	pathogen					166:173	the causative pathogen	152:173	the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide	152:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	1	25	theme	pandemic	191:198	arg1	coronavirus					135:145	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	6	26	theme	different	1016:1024	arg1	patterns					1062:1069	different protein glycosylation and secretion patterns	1016:1069	different protein glycosylation and secretion patterns	1016:1069	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	6	26	theme	different	1016:1024	arg1	shedding					1082:1089	shedding	1082:1089	shedding of spike monomeric subunits for the non-stabilized wild-type spike tested	1082:1163	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	1	27	theme	Severe	101:106	arg1	pathogen					166:173	the causative pathogen	152:173	the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide	152:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	1	27	theme	Severe	101:106	arg1	coronavirus					135:145	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	5	28	theme	engineered	902:911	arg1	glycoproteins					919:931	engineered spike glycoproteins	902:931	engineered spike glycoproteins	902:931	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	5	29	theme	tPA	850:852	arg1	sequence					861:868	tPA signal sequence	850:868	tPA signal sequence	850:868	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	6	30	theme	non-stabilized	1127:1140	arg1	spike					1152:1156	the non-stabilized wild-type spike	1123:1156	the non-stabilized wild-type spike tested	1123:1163	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	1	31	theme	acute	108:112	arg1	pathogen					166:173	the causative pathogen	152:173	the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide	152:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	1	31	theme	acute	108:112	arg1	coronavirus					135:145	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	4	32	theme	glycoprotein-based	645:662	arg1	candidates					672:681	glycoprotein-based vaccine candidates	645:681	glycoprotein-based vaccine candidates	645:681	Therefore, immunogen integrity is important for glycoprotein-based vaccine candidates.					
34056088	0	33	theme	Structural	18:27	arg1	Integrity					29:37	Antigen Structural Integrity	10:37	Antigen Structural Integrity	10:37	Assessing Antigen Structural Integrity through Glycosylation Analysis of the SARS-CoV-2 Viral Spike.					
34056088	3	34	theme	immune	436:441	arg1	evasion					443:449	immune evasion	436:449	immune evasion	436:449	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	5	35	with	plasmid	818:824	arg1	sequence					861:868	tPA signal sequence	850:868	tPA signal sequence	850:868	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	5	35	with	plasmid	818:824	arg1	promoter					837:844	a CMV promoter	831:844	a CMV promoter	831:844	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	1	36	theme	respiratory	114:124	arg1	pathogen					166:173	the causative pathogen	152:173	the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide	152:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	1	36	theme	respiratory	114:124	arg1	coronavirus					135:145	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	3	37	theme	specific	464:471	arg1	epitopes					481:488	specific protein epitopes	464:488	specific protein epitopes	464:488	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	0	38	theme	Glycosylation	47:59	arg1	Analysis					61:68	Glycosylation Analysis	47:68	Glycosylation Analysis of the SARS-CoV-2 Viral Spike	47:98	Assessing Antigen Structural Integrity through Glycosylation Analysis of the SARS-CoV-2 Viral Spike.					
34056088	5	39	theme	virus-derived	746:758	arg1	spikes					760:765	virus-derived spikes	746:765	virus-derived spikes	746:765	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	6	40	contain	have	1176:1179	arg2	implications					1181:1192	implications	1181:1192	implications for the resulting immune response and vaccine design	1181:1245	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	6	40	contain	have	1176:1179	arg1	which					1166:1170	which	1166:1170	which	1166:1170	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	1	41	contain	has	228:230	arg2	worldwide					256:264	claimed 2 776 175 lives worldwide	232:264	claimed 2 776 175 lives worldwide	232:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	1	41	contain	has	228:230	arg1	pathogen					166:173	the causative pathogen	152:173	the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide	152:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	1	41	contain	has	228:230	arg1	coronavirus					135:145	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	6	42	theme	spike	1094:1098	arg1	subunits					1110:1117	spike monomeric subunits	1094:1117	spike monomeric subunits	1094:1117	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	5	43	theme	used	884:887	arg1	recombinant					889:899	commonly used recombinant	875:899	commonly used recombinant	875:899	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	6	44	theme	secretion	987:995	arg1	pathways					997:1004	their distinctive cellular secretion pathways	960:1004	their distinctive cellular secretion pathways	960:1004	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	4	45	theme	vaccine	664:670	arg1	candidates					672:681	glycoprotein-based vaccine candidates	645:681	glycoprotein-based vaccine candidates	645:681	Therefore, immunogen integrity is important for glycoprotein-based vaccine candidates.					
34056088	5	46	theme	spike	913:917	arg1	glycoproteins					919:931	engineered spike glycoproteins	902:931	engineered spike glycoproteins	902:931	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	2	47	gly	glycoprotein	329:340	arg1	glycoprotein					329:340	the viral trimeric spike glycoprotein	304:340	the viral trimeric spike glycoprotein as the main target of the humoral immune response	304:390	Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.					
34056088	1	48	theme	syndrome	126:133	arg1	pathogen					166:173	the causative pathogen	152:173	the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide	152:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	1	48	theme	syndrome	126:133	arg1	coronavirus					135:145	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	3	49	theme	Viral	393:397	arg1	spikes					399:404	Viral spikes	393:404	Viral spikes	393:404	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	6	50	theme	distinctive	966:976	arg1	pathways					997:1004	their distinctive cellular secretion pathways	960:1004	their distinctive cellular secretion pathways	960:1004	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	3	51	theme	protein	473:479	arg1	epitopes					481:488	specific protein epitopes	464:488	specific protein epitopes	464:488	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	6	52	theme	resulting	1202:1210	arg1	response					1219:1226	the resulting immune response	1198:1226	the resulting immune response	1198:1226	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	3	53	theme	antibody	495:502	arg1	neutralization					504:517	antibody neutralization	495:517	antibody neutralization	495:517	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	5	54	theme	site-specific	702:714	arg1	glycosylation					716:728	site-specific glycosylation	702:728	site-specific glycosylation	702:728	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	6	55	theme	vaccine	1232:1238	arg1	design					1240:1245	vaccine design	1232:1245	vaccine design	1232:1245	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	2	56	theme	spike	323:327	arg1	glycoprotein					329:340	the viral trimeric spike glycoprotein	304:340	the viral trimeric spike glycoprotein as the main target of the humoral immune response	304:390	Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.					
34056088	3	57	theme	spike	558:562	arg1	production					577:586	spike glycoprotein production	558:586	spike glycoprotein production in vivo	558:594	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	5	58	dep	wild-type	768:776	arg1	non-stabilized					779:792	non-stabilized	779:792	non-stabilized	779:792	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	2	59	theme	trimeric	314:321	arg1	glycoprotein					329:340	the viral trimeric spike glycoprotein	304:340	the viral trimeric spike glycoprotein as the main target of the humoral immune response	304:390	Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.					
34056088	3	60	gly	glycoprotein	564:575	arg1	glycoprotein					564:575	spike glycoprotein production	558:586	spike glycoprotein production in vivo	558:594	Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralization, and antigen efficacy is influenced by spike glycoprotein production in vivo.					
34056088	0	61	theme	SARS-CoV-2	77:86	arg1	Spike					94:98	the SARS-CoV-2 Viral Spike	73:98	the SARS-CoV-2 Viral Spike	73:98	Assessing Antigen Structural Integrity through Glycosylation Analysis of the SARS-CoV-2 Viral Spike.					
34056088	6	62	theme	subunits	1110:1117	arg1	shedding					1082:1089	shedding	1082:1089	shedding of spike monomeric subunits for the non-stabilized wild-type spike tested	1082:1163	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	5	63	link	virus-derived	746:758	arg1	spikes					760:765	virus-derived spikes	746:765	virus-derived spikes	746:765	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	2	64	theme	immune	376:381	arg1	response					383:390	the humoral immune response	364:390	the humoral immune response	364:390	Vaccine development efforts focus on the viral trimeric spike glycoprotein as the main target of the humoral immune response.					
34056088	6	65	theme	glycosylation	1034:1046	arg1	patterns					1062:1069	different protein glycosylation and secretion patterns	1016:1069	different protein glycosylation and secretion patterns	1016:1069	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	6	65	theme	glycosylation	1034:1046	arg1	shedding					1082:1089	shedding	1082:1089	shedding of spike monomeric subunits for the non-stabilized wild-type spike tested	1082:1163	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34056088	5	66	theme	signal	854:859	arg1	sequence					861:868	tPA signal sequence	850:868	tPA signal sequence	850:868	Here, we show how site-specific glycosylation differs between virus-derived spikes, wild-type, non-stabilized spikes expressed from a plasmid with a CMV promoter and tPA signal sequence, and commonly used recombinant, engineered spike glycoproteins.					
34056088	1	67	theme	causative	156:164	arg1	pathogen					166:173	the causative pathogen	152:173	the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide	152:264	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	1	67	theme	causative	156:164	arg1	coronavirus					135:145	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2	101:147	Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of March 29, 2021, has claimed 2 776 175 lives worldwide.					
34056088	6	68	theme	cellular	978:985	arg1	pathways					997:1004	their distinctive cellular secretion pathways	960:1004	their distinctive cellular secretion pathways	960:1004	Furthermore, we show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike tested, which may have implications for the resulting immune response and vaccine design.					
34925381	0	0	theme	Spike	53:57	arg1	Protein					59:65	the Spike Protein	49:65	the Spike Protein	49:65	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	5	1	theme	universal	1275:1283	arg1	vaccines					1294:1301	universal COVID-19 vaccines	1275:1301	universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants	1275:1357	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	3	2	theme	glycan-masking	784:797	arg1	Ad-S-R158N/Y160T					799:814	glycan-masking Ad-S-R158N/Y160T	784:814	glycan-masking Ad-S-R158N/Y160T	784:814	Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold, and 4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants, respectively.					
34925381	0	3	from	Masking	7:13	arg1	RBD					42:44	RBD	42:44	RBD	42:44	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	0	3	from	Masking	7:13	arg1	NTD					34:36	NTD	34:36	NTD	34:36	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	2	4	theme	antibody	586:593	arg1	titers					595:600	the neutralizing antibody titers	569:600	the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain	569:640	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	1	5	theme	vaccine	155:161	arg1	antigen					163:169	the vaccine antigen	151:169	the vaccine antigen	151:169	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	3	6	theme	Ad-S-R158N/Y160T	799:814	arg1	use					777:779	the use	773:779	the use of glycan-masking Ad-S-R158N/Y160T in the NTD	773:825	Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold, and 4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants, respectively.					
34925381	4	7	theme	2.0-fold	1059:1066	arg1	increase					1068:1075	a 3.0-fold and 2.0-fold increase	1044:1075	a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants	1044:1165	Glycan-masking of Ad-S-D428N in the RBD resulted in a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants, respectively.					
34925381	2	8	theme	neutralizing	573:584	arg1	titers					595:600	the neutralizing antibody titers	569:600	the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain	569:640	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	3	9	theme	Beta	931:934	arg1	variants					968:975	the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants	910:975	the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants	910:975	Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold, and 4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants, respectively.					
34925381	0	10	theme	Protein	59:65	arg1	RBD					42:44	RBD	42:44	RBD	42:44	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	0	10	theme	Protein	59:65	arg1	NTD					34:36	NTD	34:36	NTD	34:36	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	4	11	theme	IC-50	1084:1088	arg1	titer					1105:1109	the IC-50 neutralization titer	1080:1109	the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants	1080:1165	Glycan-masking of Ad-S-D428N in the RBD resulted in a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants, respectively.					
34925381	1	12	gly	glycosylation	241:253	arg2	motif					255:259	an additional N-linked glycosylation motif	218:259	an additional N-linked glycosylation motif	218:259	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	3	13	theme	4.6-fold	862:869	arg1	increase					871:878	4.6-fold increase	862:878	4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants	862:975	Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold, and 4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants, respectively.					
34925381	1	14	theme	B-cell	273:278	arg1	responses					280:288	B-cell responses	273:288	B-cell responses to desired epitopes	273:308	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	0	15	theme	Neutralizing	83:94	arg1	Antibodies					96:105	Broadly Neutralizing Antibodies	75:105	Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants	75:133	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	0	16	from	NTD	34:36	arg1	Masking					7:13	Glycan Masking	0:13	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein	0:65	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	2	17	theme	ancestral	625:633	arg1	strain					635:640	the Wuhan-Hu-1 ancestral strain	610:640	the Wuhan-Hu-1 ancestral strain	610:640	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	4	18	theme	Beta	1143:1146	arg1	variants					1158:1165	the Alpha (B.1.1.7) and Beta (B.1.351) variants	1119:1165	the Alpha (B.1.1.7) and Beta (B.1.351) variants	1119:1165	Glycan-masking of Ad-S-D428N in the RBD resulted in a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants, respectively.					
34925381	2	19	theme	glycan-masking	403:416	arg1	mutants					418:424	glycan-masking mutants	403:424	glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2	403:503	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	0	20	theme	Broadly	75:81	arg1	Antibodies					96:105	Broadly Neutralizing Antibodies	75:105	Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants	75:133	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	2	21	theme	Wuhan-Hu-1	614:623	arg1	strain					635:640	the Wuhan-Hu-1 ancestral strain	610:640	the Wuhan-Hu-1 ancestral strain	610:640	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	1	22	link	N-linked	232:239	arg1	motif					255:259	an additional N-linked glycosylation motif	218:259	an additional N-linked glycosylation motif	218:259	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	3	23	from	use	777:779	arg1	NTD					823:825	the NTD	819:825	the NTD	819:825	Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold, and 4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants, respectively.					
34925381	5	24	theme	antigen	1243:1249	arg1	design					1251:1256	antigen design	1243:1256	antigen design	1243:1256	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	5	25	theme	future	1323:1328	arg1	variants					1350:1357	current and future emerging SARS-CoV-2 variants	1311:1357	current and future emerging SARS-CoV-2 variants	1311:1357	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	5	26	from	Ad-S-R158N/Y160T	1211:1226	arg1	use					1186:1188	The use	1182:1188	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design	1182:1256	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	4	27	theme	3.0-fold	1046:1053	arg1	increase					1068:1075	a 3.0-fold and 2.0-fold increase	1044:1075	a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants	1044:1165	Glycan-masking of Ad-S-D428N in the RBD resulted in a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants, respectively.					
34925381	0	28	theme	Glycan	0:5	arg1	Masking					7:13	Glycan Masking	0:13	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein	0:65	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	2	29	theme	SARS-CoV-2	667:676	arg1	Delta					724:728	Delta (B.1.617.2)	724:740	Delta (B.1.617.2)	724:740	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	29	theme	SARS-CoV-2	667:676	arg1	variants					678:685	the recently emerged SARS-CoV-2 variants	646:685	the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2)	646:740	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	29	theme	SARS-CoV-2	667:676	arg1	Beta					704:707	Beta (B.1.351)	704:717	Beta (B.1.351)	704:717	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	29	theme	SARS-CoV-2	667:676	arg1	Alpha					687:691	Alpha (B.1.1.7)	687:701	Alpha (B.1.1.7)	687:701	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	5	30	from	use	1186:1188	arg1	Ad-S-D428N					1232:1241	Ad-S-D428N	1232:1241	Ad-S-D428N	1232:1241	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	5	30	from	use	1186:1188	arg1	Ad-S-R158N/Y160T					1211:1226	Ad-S-R158N/Y160T	1211:1226	Ad-S-R158N/Y160T	1211:1226	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	3	31	theme	IC-50	887:891	arg1	titer					896:900	the IC-50 NT titer	883:900	the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants	883:975	Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold, and 4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants, respectively.					
34925381	0	32	theme	Epitopes	18:25	arg1	Masking					7:13	Glycan Masking	0:13	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein	0:65	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	5	33	theme	SARS-CoV-2	1339:1348	arg1	variants					1350:1357	current and future emerging SARS-CoV-2 variants	1311:1357	current and future emerging SARS-CoV-2 variants	1311:1357	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	1	34	with	sites	207:211	arg1	motif					255:259	an additional N-linked glycosylation motif	218:259	an additional N-linked glycosylation motif	218:259	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	2	35	theme	emerged	659:665	arg1	Delta					724:728	Delta (B.1.617.2)	724:740	Delta (B.1.617.2)	724:740	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	35	theme	emerged	659:665	arg1	variants					678:685	the recently emerged SARS-CoV-2 variants	646:685	the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2)	646:740	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	35	theme	emerged	659:665	arg1	Beta					704:707	Beta (B.1.351)	704:717	Beta (B.1.351)	704:717	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	35	theme	emerged	659:665	arg1	Alpha					687:691	Alpha (B.1.1.7)	687:701	Alpha (B.1.1.7)	687:701	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	3	36	theme	NT	893:894	arg1	titer					896:900	the IC-50 NT titer	883:900	the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants	883:975	Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold, and 4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants, respectively.					
34925381	1	37	theme	undesired	187:195	arg1	sites					207:211	the undesired antigenic sites	183:211	the undesired antigenic sites with an additional N-linked glycosylation motif	183:259	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	2	38	theme	domain	444:449	arg1	mutants					418:424	glycan-masking mutants	403:424	glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2	403:503	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	1	39	theme	desired	293:299	arg1	epitopes					301:308	desired epitopes	293:308	desired epitopes	293:308	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	1	40	theme	antigenic	197:205	arg1	sites					207:211	the undesired antigenic sites	183:211	the undesired antigenic sites with an additional N-linked glycosylation motif	183:259	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	2	41	theme	antigenic	525:533	arg1	design					535:540	the antigenic design	521:540	the antigenic design of the S protein	521:557	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	1	42	theme	overall-folded	343:356	arg1	structure					358:366	the antigen's overall-folded structure	329:366	the antigen's overall-folded structure	329:366	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	5	43	from	Ad-S-D428N	1232:1241	arg1	use					1186:1188	The use	1182:1188	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design	1182:1256	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	2	44	theme	domain	478:483	arg1	mutants					418:424	glycan-masking mutants	403:424	glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2	403:503	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	0	45	theme	SARS-CoV-2	115:124	arg1	Variants					126:133	SARS-CoV-2 Variants	115:133	SARS-CoV-2 Variants	115:133	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	0	46	dep	NTD	34:36	arg1	the					30:32	the	30:32	the	30:32	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	4	47	theme	neutralization	1090:1103	arg1	titer					1105:1109	the IC-50 neutralization titer	1080:1109	the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants	1080:1165	Glycan-masking of Ad-S-D428N in the RBD resulted in a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants, respectively.					
34925381	2	48	theme	mutants	418:424	arg1	impact					393:398	the impact	389:398	the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2	389:503	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	49	theme	receptor-binding	461:476	arg1	RBD					486:488	RBD	486:488	RBD	486:488	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	49	theme	receptor-binding	461:476	arg1	SARS-CoV-2					494:503	SARS-CoV-2	494:503	SARS-CoV-2	494:503	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	49	theme	receptor-binding	461:476	arg1	domain					478:483	receptor-binding domain	461:483	receptor-binding domain (RBD) of SARS-CoV-2	461:503	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	0	50	from	RBD	42:44	arg1	Masking					7:13	Glycan Masking	0:13	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein	0:65	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	3	51	theme	Alpha	914:918	arg1	variants					968:975	the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants	910:975	the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants	910:975	Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold, and 4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants, respectively.					
34925381	5	52	theme	emerging	1330:1337	arg1	variants					1350:1357	current and future emerging SARS-CoV-2 variants	1311:1357	current and future emerging SARS-CoV-2 variants	1311:1357	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	2	53	theme	protein	551:557	arg1	design					535:540	the antigenic design	521:540	the antigenic design of the S protein	521:557	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	5	54	from	glycan-masking	1193:1206	arg1	Ad-S-D428N					1232:1241	Ad-S-D428N	1232:1241	Ad-S-D428N	1232:1241	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	5	54	from	glycan-masking	1193:1206	arg1	Ad-S-R158N/Y160T					1211:1226	Ad-S-R158N/Y160T	1211:1226	Ad-S-R158N/Y160T	1211:1226	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	3	55	theme	Delta	950:954	arg1	variants					968:975	the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants	910:975	the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants	910:975	Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold, and 4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants, respectively.					
34925381	2	56	theme	S	549:549	arg1	protein					551:557	the S protein	545:557	the S protein	545:557	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	4	57	theme	Alpha	1123:1127	arg1	variants					1158:1165	the Alpha (B.1.1.7) and Beta (B.1.351) variants	1119:1165	the Alpha (B.1.1.7) and Beta (B.1.351) variants	1119:1165	Glycan-masking of Ad-S-D428N in the RBD resulted in a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants, respectively.					
34925381	5	58	theme	current	1311:1317	arg1	variants					1350:1357	current and future emerging SARS-CoV-2 variants	1311:1357	current and future emerging SARS-CoV-2 variants	1311:1357	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	4	59	from	Glycan-masking	992:1005	arg1	RBD					1028:1030	the RBD	1024:1030	the RBD	1024:1030	Glycan-masking of Ad-S-D428N in the RBD resulted in a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants, respectively.					
34925381	5	60	dep	Ad-S-R158N/Y160T	1211:1226	arg1	design					1251:1256	antigen design	1243:1256	antigen design	1243:1256	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	5	61	theme	COVID-19	1285:1292	arg1	vaccines					1294:1301	universal COVID-19 vaccines	1275:1301	universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants	1275:1357	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	5	62	theme	glycan-masking	1193:1206	arg1	use					1186:1188	The use	1182:1188	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design	1182:1256	The use of glycan-masking in Ad-S-R158N/Y160T and Ad-S-D428N antigen design may help develop universal COVID-19 vaccines against current and future emerging SARS-CoV-2 variants.					
34925381	4	63	theme	Ad-S-D428N	1010:1019	arg1	Glycan-masking					992:1005	Glycan-masking	992:1005	Glycan-masking of Ad-S-D428N in the RBD	992:1030	Glycan-masking of Ad-S-D428N in the RBD resulted in a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants, respectively.					
34925381	1	64	theme	additional	221:230	arg1	motif					255:259	an additional N-linked glycosylation motif	218:259	an additional N-linked glycosylation motif	218:259	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	2	65	theme	SARS-CoV-2	494:503	arg1	NTD					452:454	NTD	452:454	NTD	452:454	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	65	theme	SARS-CoV-2	494:503	arg1	domain					444:449	the N-terminal domain	429:449	the N-terminal domain (NTD)	429:455	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	65	theme	SARS-CoV-2	494:503	arg1	SARS-CoV-2					494:503	SARS-CoV-2	494:503	SARS-CoV-2	494:503	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	65	theme	SARS-CoV-2	494:503	arg1	domain					478:483	receptor-binding domain	461:483	receptor-binding domain (RBD) of SARS-CoV-2	461:503	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	65	theme	SARS-CoV-2	494:503	arg1	RBD					486:488	RBD	486:488	RBD	486:488	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	4	66	from	increase	1068:1075	arg1	titer					1105:1109	the IC-50 neutralization titer	1080:1109	the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants	1080:1165	Glycan-masking of Ad-S-D428N in the RBD resulted in a 3.0-fold and 2.0-fold increase in the IC-50 neutralization titer against the Alpha (B.1.1.7) and Beta (B.1.351) variants, respectively.					
34925381	2	67	theme	N-terminal	433:442	arg1	NTD					452:454	NTD	452:454	NTD	452:454	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	67	theme	N-terminal	433:442	arg1	domain					444:449	the N-terminal domain	429:449	the N-terminal domain (NTD)	429:455	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	67	theme	N-terminal	433:442	arg1	SARS-CoV-2					494:503	SARS-CoV-2	494:503	SARS-CoV-2	494:503	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	68	dep	variants	678:685	arg1	Delta					724:728	Delta (B.1.617.2)	724:740	Delta (B.1.617.2)	724:740	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	68	dep	variants	678:685	arg1	variants					678:685	the recently emerged SARS-CoV-2 variants	646:685	the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2)	646:740	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	68	dep	variants	678:685	arg1	Beta					704:707	Beta (B.1.351)	704:717	Beta (B.1.351)	704:717	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	2	68	dep	variants	678:685	arg1	Alpha					687:691	Alpha (B.1.1.7)	687:701	Alpha (B.1.1.7)	687:701	This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2).					
34925381	1	69	theme	N-linked	232:239	arg1	motif					255:259	an additional N-linked glycosylation motif	218:259	an additional N-linked glycosylation motif	218:259	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
34925381	0	70	from	Epitopes	18:25	arg1	RBD					42:44	RBD	42:44	RBD	42:44	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	0	70	from	Epitopes	18:25	arg1	NTD					34:36	NTD	34:36	NTD	34:36	Glycan Masking of Epitopes in the NTD and RBD of the Spike Protein Elicits Broadly Neutralizing Antibodies Against SARS-CoV-2 Variants.					
34925381	3	71	from	increase	871:878	arg1	titer					896:900	the IC-50 NT titer	883:900	the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants	883:975	Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold, and 4.6-fold increase in the IC-50 NT titer against the Alpha (B.1.1.7), Beta (B.1.351) and Delta (B.1.617.2) variants, respectively.					
34925381	1	72	theme	glycosylation	241:253	arg1	motif					255:259	an additional N-linked glycosylation motif	218:259	an additional N-linked glycosylation motif	218:259	Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional N-linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure.					
32507163	0	0	theme	acetate	101:107	arg1	composites					109:118	ethylene vinyl acetate composites	86:118	ethylene vinyl acetate composites	86:118	Enzymatic polymerization derived engineered polysaccharides as reinforcing fillers of ethylene vinyl acetate composites.					
32507163	4	1	theme	acetate	549:555	arg1	co-polymer					557:566	ethylene vinyl acetate co-polymer	534:566	ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40%	534:623	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	4	1	theme	acetate	549:555	arg1	EVA					569:571	EVA	569:571	EVA	569:571	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	5	2	theme	mechanical	676:685	arg1	properties					703:712	Various mechanical and rheological properties	668:712	Various mechanical and rheological properties of the fabricated composites	668:741	Various mechanical and rheological properties of the fabricated composites were evaluated.					
32507163	7	3	theme	key	1029:1031	arg1	hardness					1104:1111	hardness	1104:1111	hardness	1104:1111	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	7	3	theme	key	1029:1031	arg1	properties					1043:1052	key composite properties	1029:1052	key composite properties	1029:1052	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	7	3	theme	key	1029:1031	arg1	modulus					1074:1080	modulus	1074:1080	modulus	1074:1080	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	7	3	theme	key	1029:1031	arg1	resistance					1088:1097	wear resistance	1083:1097	wear resistance	1083:1097	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	7	3	theme	key	1029:1031	arg1	toughness					1063:1071	toughness	1063:1071	toughness	1063:1071	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	0	4	theme	vinyl	95:99	arg1	acetate					101:107	ethylene vinyl acetate	86:107	ethylene vinyl acetate composites	86:118	Enzymatic polymerization derived engineered polysaccharides as reinforcing fillers of ethylene vinyl acetate composites.					
32507163	7	5	theme	polysaccharides	1167:1181	arg1	potential					1137:1145	the reinforcing potential	1121:1145	the reinforcing potential of these engineered polysaccharides	1121:1181	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	4	6	theme	%	615:615	arg1	content					602:608	vinyl acetate content	588:608	vinyl acetate content of 32% and 40%	588:623	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	5	7	theme	rheological	691:701	arg1	properties					703:712	Various mechanical and rheological properties	668:712	Various mechanical and rheological properties of the fabricated composites	668:741	Various mechanical and rheological properties of the fabricated composites were evaluated.					
32507163	2	8	theme	various	347:353	arg1	techniques					355:364	various techniques	347:364	various techniques	347:364	The structure and material properties of the engineered polysaccharide were characterized using various techniques.					
32507163	4	9	theme	%	623:623	arg1	content					602:608	vinyl acetate content	588:608	vinyl acetate content of 32% and 40%	588:623	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	0	10	theme	composites	109:118	arg1	polysaccharides					44:58	engineered polysaccharides	33:58	engineered polysaccharides	33:58	Enzymatic polymerization derived engineered polysaccharides as reinforcing fillers of ethylene vinyl acetate composites.					
32507163	0	10	theme	composites	109:118	arg1	fillers					75:81	reinforcing fillers	63:81	reinforcing fillers of ethylene vinyl acetate composites	63:118	Enzymatic polymerization derived engineered polysaccharides as reinforcing fillers of ethylene vinyl acetate composites.					
32507163	6	11	theme	alpha-1,3	836:844	arg1	glucan					846:851	alpha-1,3 glucan	836:851	alpha-1,3 glucan that result in various performances attributes	836:898	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes was also investigated and reported.					
32507163	3	12	theme	polysaccharide	394:407	arg1	use					371:373	The use	367:373	The use of such engineered polysaccharide as a reinforcing filler of polymers	367:443	The use of such engineered polysaccharide as a reinforcing filler of polymers was evaluated using model polymers.					
32507163	7	13	theme	reinforcing	1125:1135	arg1	potential					1137:1145	the reinforcing potential	1121:1145	the reinforcing potential of these engineered polysaccharides	1121:1181	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	4	14	theme	fabrication	647:657	arg1	process					659:665	a melt processing fabrication process	629:665	a melt processing fabrication process	629:665	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	4	15	theme	vinyl	543:547	arg1	acetate					549:555	ethylene vinyl acetate	534:555	ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40%	534:623	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	7	16	theme	EVA	983:985	arg1	composites					987:996	these EVA composites	977:996	these EVA composites	977:996	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	2	17	theme	polysaccharide	307:320	arg1	structure					255:263	structure	255:263	structure	255:263	The structure and material properties of the engineered polysaccharide were characterized using various techniques.					
32507163	2	17	theme	polysaccharide	307:320	arg1	properties					278:287	material properties	269:287	material properties	269:287	The structure and material properties of the engineered polysaccharide were characterized using various techniques.					
32507163	3	18	theme	polymers	436:443	arg1	filler					426:431	a reinforcing filler	412:431	a reinforcing filler of polymers	412:443	The use of such engineered polysaccharide as a reinforcing filler of polymers was evaluated using model polymers.					
32507163	4	19	theme	ethylene	534:541	arg1	acetate					549:555	ethylene vinyl acetate	534:555	ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40%	534:623	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	3	20	theme	such	378:381	arg1	polysaccharide					394:407	such engineered polysaccharide	378:407	such engineered polysaccharide	378:407	The use of such engineered polysaccharide as a reinforcing filler of polymers was evaluated using model polymers.					
32507163	7	21	from	incorporation	940:952	arg1	composites					987:996	these EVA composites	977:996	these EVA composites	977:996	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	0	22	theme	Enzymatic	0:8	arg1	polymerization					10:23	Enzymatic polymerization	0:23	Enzymatic polymerization	0:23	Enzymatic polymerization derived engineered polysaccharides as reinforcing fillers of ethylene vinyl acetate composites.					
32507163	5	23	theme	Various	668:674	arg1	properties					703:712	Various mechanical and rheological properties	668:712	Various mechanical and rheological properties of the fabricated composites	668:741	Various mechanical and rheological properties of the fabricated composites were evaluated.					
32507163	1	24	theme	engineered	207:216	arg1	polysaccharide					235:248	an engineered alpha-1,3 glucan polysaccharide	204:248	an engineered alpha-1,3 glucan polysaccharide	204:248	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	0	25	theme	engineered	33:42	arg1	polysaccharides					44:58	engineered polysaccharides	33:58	engineered polysaccharides	33:58	Enzymatic polymerization derived engineered polysaccharides as reinforcing fillers of ethylene vinyl acetate composites.					
32507163	0	25	theme	engineered	33:42	arg1	fillers					75:81	reinforcing fillers	63:81	reinforcing fillers of ethylene vinyl acetate composites	63:118	Enzymatic polymerization derived engineered polysaccharides as reinforcing fillers of ethylene vinyl acetate composites.					
32507163	4	26	theme	acetate	594:600	arg1	content					602:608	vinyl acetate content	588:608	vinyl acetate content of 32% and 40%	588:623	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	7	27	theme	engineered	1156:1165	arg1	polysaccharides					1167:1181	these engineered polysaccharides	1150:1181	these engineered polysaccharides	1150:1181	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	6	28	theme	resin	806:810	arg1	content					787:793	vinyl acetate content	773:793	vinyl acetate content of the EVA resin	773:810	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes was also investigated and reported.					
32507163	4	29	with	matrices	574:581	arg1	content					602:608	vinyl acetate content	588:608	vinyl acetate content of 32% and 40%	588:623	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	2	30	dep	structure	255:263	arg1	The					251:253	The	251:253	The	251:253	The structure and material properties of the engineered polysaccharide were characterized using various techniques.					
32507163	6	31	theme	vinyl	773:777	arg1	acetate					779:785	vinyl acetate	773:785	vinyl acetate content of the EVA resin	773:810	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes was also investigated and reported.					
32507163	4	32	theme	alpha-1,3	495:503	arg1	glucan					505:510	the alpha-1,3 glucan	491:510	the alpha-1,3 glucan	491:510	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	1	33	theme	novel	123:127	arg1	polymerization					165:178	controlled enzymatic polymerization	144:178	controlled enzymatic polymerization	144:178	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	1	33	theme	novel	123:127	arg1	based					137:141	A novel monomer based	121:141	A novel monomer based	121:141	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	1	34	theme	glucan	228:233	arg1	polysaccharide					235:248	an engineered alpha-1,3 glucan polysaccharide	204:248	an engineered alpha-1,3 glucan polysaccharide	204:248	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	3	35	theme	model	465:469	arg1	polymers					471:478	model polymers	465:478	model polymers	465:478	The use of such engineered polysaccharide as a reinforcing filler of polymers was evaluated using model polymers.					
32507163	2	36	theme	material	269:276	arg1	properties					278:287	material properties	269:287	material properties	269:287	The structure and material properties of the engineered polysaccharide were characterized using various techniques.					
32507163	6	37	theme	EVA	802:804	arg1	resin					806:810	the EVA resin	798:810	the EVA resin	798:810	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes was also investigated and reported.					
32507163	7	38	theme	composite	1033:1041	arg1	hardness					1104:1111	hardness	1104:1111	hardness	1104:1111	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	7	38	theme	composite	1033:1041	arg1	properties					1043:1052	key composite properties	1029:1052	key composite properties	1029:1052	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	7	38	theme	composite	1033:1041	arg1	modulus					1074:1080	modulus	1074:1080	modulus	1074:1080	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	7	38	theme	composite	1033:1041	arg1	resistance					1088:1097	wear resistance	1083:1097	wear resistance	1083:1097	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	7	38	theme	composite	1033:1041	arg1	toughness					1063:1071	toughness	1063:1071	toughness	1063:1071	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	6	39	with	interaction	819:829	arg1	glucan					846:851	alpha-1,3 glucan	836:851	alpha-1,3 glucan that result in various performances attributes	836:898	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes was also investigated and reported.					
32507163	1	40	theme	monomer	129:135	arg1	polymerization					165:178	controlled enzymatic polymerization	144:178	controlled enzymatic polymerization	144:178	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	1	40	theme	monomer	129:135	arg1	based					137:141	A novel monomer based	121:141	A novel monomer based	121:141	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	0	41	theme	reinforcing	63:73	arg1	polysaccharides					44:58	engineered polysaccharides	33:58	engineered polysaccharides	33:58	Enzymatic polymerization derived engineered polysaccharides as reinforcing fillers of ethylene vinyl acetate composites.					
32507163	0	41	theme	reinforcing	63:73	arg1	fillers					75:81	reinforcing fillers	63:81	reinforcing fillers of ethylene vinyl acetate composites	63:118	Enzymatic polymerization derived engineered polysaccharides as reinforcing fillers of ethylene vinyl acetate composites.					
32507163	4	42	theme	vinyl	588:592	arg1	acetate					594:600	vinyl acetate	588:600	vinyl acetate content of 32% and 40%	588:623	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	7	43	theme	wear	1083:1086	arg1	resistance					1088:1097	wear resistance	1083:1097	wear resistance	1083:1097	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	6	44	theme	performances	876:887	arg1	attributes					889:898	various performances attributes	868:898	various performances attributes	868:898	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes was also investigated and reported.					
32507163	7	45	theme	alpha-1,3	957:965	arg1	glucan					967:972	alpha-1,3 glucan	957:972	alpha-1,3 glucan	957:972	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	7	46	theme	properties	1043:1052	arg1	improvement					1014:1024	the improvement	1010:1024	the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides	1010:1181	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	5	47	theme	fabricated	721:730	arg1	composites					732:741	the fabricated composites	717:741	the fabricated composites	717:741	Various mechanical and rheological properties of the fabricated composites were evaluated.					
32507163	6	48	theme	various	868:874	arg1	attributes					889:898	various performances attributes	868:898	various performances attributes	868:898	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes was also investigated and reported.					
32507163	2	49	theme	engineered	296:305	arg1	polysaccharide					307:320	the engineered polysaccharide	292:320	the engineered polysaccharide	292:320	The structure and material properties of the engineered polysaccharide were characterized using various techniques.					
32507163	6	50	theme	content	787:793	arg1	effect					763:768	The effect	759:768	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes	759:898	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes was also investigated and reported.					
32507163	7	51	theme	glucan	967:972	arg1	incorporation					940:952	The incorporation	936:952	The incorporation of alpha-1,3 glucan in these EVA composites	936:996	The incorporation of alpha-1,3 glucan in these EVA composites resulted in the improvement of key composite properties, such as toughness, modulus, wear resistance, and hardness showing the reinforcing potential of these engineered polysaccharides.					
32507163	5	52	theme	composites	732:741	arg1	properties					703:712	Various mechanical and rheological properties	668:712	Various mechanical and rheological properties of the fabricated composites	668:741	Various mechanical and rheological properties of the fabricated composites were evaluated.					
32507163	6	53	theme	acetate	779:785	arg1	content					787:793	vinyl acetate content	773:793	vinyl acetate content of the EVA resin	773:810	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes was also investigated and reported.					
32507163	1	54	theme	controlled	144:153	arg1	polymerization					165:178	controlled enzymatic polymerization	144:178	controlled enzymatic polymerization	144:178	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	1	54	theme	controlled	144:153	arg1	based					137:141	A novel monomer based	121:141	A novel monomer based	121:141	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	0	55	theme	ethylene	86:93	arg1	acetate					101:107	ethylene vinyl acetate	86:107	ethylene vinyl acetate composites	86:118	Enzymatic polymerization derived engineered polysaccharides as reinforcing fillers of ethylene vinyl acetate composites.					
32507163	1	56	theme	alpha-1,3	218:226	arg1	polysaccharide					235:248	an engineered alpha-1,3 glucan polysaccharide	204:248	an engineered alpha-1,3 glucan polysaccharide	204:248	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	1	57	theme	enzymatic	155:163	arg1	polymerization					165:178	controlled enzymatic polymerization	144:178	controlled enzymatic polymerization	144:178	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	1	57	theme	enzymatic	155:163	arg1	based					137:141	A novel monomer based	121:141	A novel monomer based	121:141	A novel monomer based, controlled enzymatic polymerization was employed to produce an engineered alpha-1,3 glucan polysaccharide.					
32507163	4	58	theme	processing	636:645	arg1	process					659:665	a melt processing fabrication process	629:665	a melt processing fabrication process	629:665	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	3	59	theme	engineered	383:392	arg1	polysaccharide					394:407	such engineered polysaccharide	378:407	such engineered polysaccharide	378:407	The use of such engineered polysaccharide as a reinforcing filler of polymers was evaluated using model polymers.					
32507163	4	60	theme	co-polymer	557:566	arg1	matrices					574:581	ethylene vinyl acetate co-polymer (EVA) matrices	534:581	ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40%	534:623	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	4	61	theme	melt	631:634	arg1	process					659:665	a melt processing fabrication process	629:665	a melt processing fabrication process	629:665	For this, the alpha-1,3 glucan was incorporated into ethylene vinyl acetate co-polymer (EVA) matrices with vinyl acetate content of 32% and 40% via a melt processing fabrication process.					
32507163	6	62	from	effect	763:768	arg1	interaction					819:829	the interaction	815:829	the interaction with alpha-1,3 glucan that result in various performances attributes	815:898	The effect of vinyl acetate content of the EVA resin on the interaction with alpha-1,3 glucan that result in various performances attributes was also investigated and reported.					
32507163	3	63	theme	reinforcing	414:424	arg1	filler					426:431	a reinforcing filler	412:431	a reinforcing filler of polymers	412:443	The use of such engineered polysaccharide as a reinforcing filler of polymers was evaluated using model polymers.					
32084323	2	0	theme	physical	320:327	arg1	force					329:333	physical force	320:333	physical force	320:333	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
32084323	0	1	theme	Walls	63:67	arg1	Perception					22:31	Perception	22:31	Perception	22:31	Dynamic Construction, Perception, and Remodeling of Plant Cell Walls.					
32084323	0	1	theme	Walls	63:67	arg1	Remodeling					38:47	Remodeling	38:47	Remodeling	38:47	Dynamic Construction, Perception, and Remodeling of Plant Cell Walls.					
32084323	0	1	theme	Walls	63:67	arg1	Construction					8:19	Dynamic Construction	0:19	Dynamic Construction	0:19	Dynamic Construction, Perception, and Remodeling of Plant Cell Walls.					
32084323	3	2	theme	cell	826:829	arg1	walls					831:835	their cell walls	820:835	their cell walls	820:835	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	2	3	theme	other	254:258	arg1	biomolecules					260:271	other biomolecules	254:271	other biomolecules	254:271	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
32084323	3	4	dep	genetics	594:601	arg1	methods					680:686	methods	680:686	methods	680:686	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	5	theme	nanoscale	886:894	arg1	assembly					896:903	the nanoscale assembly	882:903	the nanoscale assembly of these remarkable structures	882:934	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	1	6	theme	durable	152:158	arg1	coverings					160:168	durable coverings	152:168	durable coverings for the delicate cells	152:191	Plant cell walls are dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase.					
32084323	2	7	theme	many	404:407	arg1	cases					409:413	many cases	404:413	many cases	404:413	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
32084323	3	8	theme	diverse	704:710	arg1	mechanisms					719:728	mechanisms	719:728	mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls	719:835	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	8	theme	diverse	704:710	arg1	set					712:714	a diverse set	702:714	a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls	702:835	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	1	9	theme	Plant	70:74	arg1	walls					81:85	Plant cell walls	70:85	Plant cell walls	70:85	Plant cell walls are dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase.					
32084323	1	9	theme	Plant	70:74	arg1	structures					99:108	dynamic structures	91:108	dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase	91:203	Plant cell walls are dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase.					
32084323	0	10	theme	Dynamic	0:6	arg1	Construction					8:19	Dynamic Construction	0:19	Dynamic Construction	0:19	Dynamic Construction, Perception, and Remodeling of Plant Cell Walls.					
32084323	1	11	theme	cell	76:79	arg1	walls					81:85	Plant cell walls	70:85	Plant cell walls	70:85	Plant cell walls are dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase.					
32084323	1	11	theme	cell	76:79	arg1	structures					99:108	dynamic structures	91:108	dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase	91:203	Plant cell walls are dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase.					
32084323	3	12	theme	plant	739:743	arg1	cells					745:749	plant cells	739:749	plant cells	739:749	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	13	theme	remarkable	914:923	arg1	structures					925:934	these remarkable structures	908:934	these remarkable structures	908:934	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	14	theme	mechanisms	719:728	arg1	mechanisms					719:728	mechanisms	719:728	mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls	719:835	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	14	theme	mechanisms	719:728	arg1	set					712:714	a diverse set	702:714	a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls	702:835	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	1	15	theme	delicate	178:185	arg1	cells					187:191	the delicate cells	174:191	the delicate cells	174:191	Plant cell walls are dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase.					
32084323	2	16	theme	developmental	501:513	arg1	transitions					515:525	developmental transitions	501:525	developmental transitions	501:525	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
32084323	3	17	theme	structural	630:639	arg1	biology					641:647	structural biology	630:647	structural biology	630:647	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	18	theme	Recent	575:580	arg1	advances					582:589	Recent advances	575:589	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods	575:686	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	19	from	advances	582:589	arg1	genetics					594:601	genetics	594:601	genetics	594:601	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	19	from	advances	582:589	arg1	characterization					663:678	physical characterization	654:678	physical characterization	654:678	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	19	from	advances	582:589	arg1	biochemistry					616:627	biochemistry	616:627	biochemistry	616:627	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	19	from	advances	582:589	arg1	biology					641:647	structural biology	630:647	structural biology	630:647	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	19	from	advances	582:589	arg1	microscopy					604:613	microscopy	604:613	microscopy	604:613	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	20	dep	composition	788:798	arg1	the					784:786	the	784:786	the	784:786	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	1	21	dep	provide	144:150	arg1	encase					198:203	encase	198:203	encase	198:203	Plant cell walls are dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase.					
32084323	3	22	theme	majestic	950:957	arg1	forms					959:963	the majestic forms	946:963	the majestic forms	946:963	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	1	23	theme	dynamic	91:97	arg1	walls					81:85	Plant cell walls	70:85	Plant cell walls	70:85	Plant cell walls are dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase.					
32084323	1	23	theme	dynamic	91:97	arg1	structures					99:108	dynamic structures	91:108	dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase	91:203	Plant cell walls are dynamic structures that are synthesized by plants to provide durable coverings for the delicate cells they encase.					
32084323	3	24	theme	structures	925:934	arg1	assembly					896:903	the nanoscale assembly	882:903	the nanoscale assembly of these remarkable structures	882:934	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	2	25	theme	large	303:307	arg1	amounts					309:315	large amounts	303:315	large amounts of physical force	303:333	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
32084323	2	25	theme	large	303:307	arg1	force					329:333	physical force	320:333	physical force	320:333	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
32084323	2	26	theme	plant	540:544	arg1	physiology					546:555	plant physiology	540:555	plant physiology	540:555	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
32084323	3	27	theme	physical	654:661	arg1	characterization					663:678	physical characterization	654:678	physical characterization	654:678	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	2	28	dep	undergo	437:443	arg1	controlled					445:454	controlled	445:454	undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction	437:572	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
32084323	3	29	theme	vital	969:973	arg1	functions					986:994	vital ecological functions	969:994	vital ecological functions achieved by plants	969:1013	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	30	theme	walls	831:835	arg1	composition					788:798	composition	788:798	composition	788:798	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	3	30	theme	walls	831:835	arg1	architecture					804:815	architecture	804:815	architecture	804:815	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	0	31	theme	Cell	58:61	arg1	Walls					63:67	Plant Cell Walls	52:67	Plant Cell Walls	52:67	Dynamic Construction, Perception, and Remodeling of Plant Cell Walls.					
32084323	3	32	theme	ecological	975:984	arg1	functions					986:994	vital ecological functions	969:994	vital ecological functions achieved by plants	969:1013	Recent advances in genetics, microscopy, biochemistry, structural biology, and physical characterization methods have revealed a diverse set of mechanisms by which plant cells dynamically monitor and regulate the composition and architecture of their cell walls, but much remains to be discovered about how the nanoscale assembly of these remarkable structures underpins the majestic forms and vital ecological functions achieved by plants.					
32084323	2	33	theme	external	349:356	arg1	attack					358:363	external attack	349:363	external attack by herbivores and pathogens	349:391	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
32084323	0	34	theme	Plant	52:56	arg1	Walls					63:67	Plant Cell Walls	52:67	Plant Cell Walls	52:67	Dynamic Construction, Perception, and Remodeling of Plant Cell Walls.					
32084323	2	35	theme	force	329:333	arg1	amounts					309:315	large amounts	303:315	large amounts of physical force	303:333	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
32084323	2	35	theme	force	329:333	arg1	force					329:333	physical force	320:333	physical force	320:333	They are made of polysaccharides, proteins, and other biomolecules and have evolved to withstand large amounts of physical force and to resist external attack by herbivores and pathogens but can in many cases expand, contract, and undergo controlled degradation and reconstruction to facilitate developmental transitions and regulate plant physiology and reproduction.					
33524890	0	0	theme	michiganensis	83:95	arg1	subsp					97:101	Clavibacter michiganensis subsp	71:101	Clavibacter michiganensis subsp	71:101	Novel galactofuranan and pyruvylated galactomannan in the cell wall of Clavibacter michiganensis subsp.					
33524890	4	1	theme	-α-D-Fucp-	492:501	arg1	trisaccharide					441:453	side trisaccharide	436:453	side trisaccharide	436:453	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	1	theme	-α-D-Fucp-	492:501	arg1	1 →					503:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	2	theme	1 → 2	486:490	arg1	trisaccharide					441:453	side trisaccharide	436:453	side trisaccharide	436:453	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	2	theme	1 → 2	486:490	arg1	1 →					503:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	7	3	theme	Clavibacter	852:862	arg1	species					831:837	plant pathogenic species	814:837	plant pathogenic species of the genus Clavibacter	814:862	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	0	4	theme	Clavibacter	71:81	arg1	subsp					97:101	Clavibacter michiganensis subsp	71:101	Clavibacter michiganensis subsp	71:101	Novel galactofuranan and pyruvylated galactomannan in the cell wall of Clavibacter michiganensis subsp.					
33524890	0	5	from	galactofuranan	6:19	arg1	wall					63:66	the cell wall	54:66	the cell wall of Clavibacter michiganensis subsp	54:101	Novel galactofuranan and pyruvylated galactomannan in the cell wall of Clavibacter michiganensis subsp.					
33524890	5	6	theme	1 → 3	624:628	arg1	1 →					641:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	6	theme	1 → 3	624:628	arg1	unit					575:578	the repeating unit	561:578	the repeating unit	561:578	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	3	7	theme	michiganensis	182:194	arg1	Ас-1403Т					200:207	michiganensis VKM Ас-1403Т	182:207	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria)	182:256	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides.					
33524890	4	8	theme	1 → 3	469:473	arg1	trisaccharide					441:453	side trisaccharide	436:453	side trisaccharide	436:453	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	8	theme	1 → 3	469:473	arg1	1 →					503:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	3	9	theme	VKM	196:198	arg1	Ас-1403Т					200:207	michiganensis VKM Ас-1403Т	182:207	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria)	182:256	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides.					
33524890	7	10	theme	plants	961:966	arg1	colonization					931:942	colonization	931:942	colonization	931:942	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	7	10	theme	plants	961:966	arg1	infection					948:956	infection	948:956	infection of plants	948:966	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	2	11	theme	Clavibacter	149:159	arg1	subsp					175:179	Clavibacter michiganensis subsp	149:179	Clavibacter michiganensis subsp	149:179	The cell wall of Clavibacter michiganensis subsp.					
33524890	0	12	theme	subsp	97:101	arg1	wall					63:66	the cell wall	54:66	the cell wall of Clavibacter michiganensis subsp	54:101	Novel galactofuranan and pyruvylated galactomannan in the cell wall of Clavibacter michiganensis subsp.					
33524890	2	13	theme	michiganensis	161:173	arg1	subsp					175:179	Clavibacter michiganensis subsp	149:179	Clavibacter michiganensis subsp	149:179	The cell wall of Clavibacter michiganensis subsp.					
33524890	3	14	theme	class	236:240	arg1	Actinobacteria					242:255	class Actinobacteria	236:255	class Actinobacteria	236:255	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides.					
33524890	3	14	theme	class	236:240	arg1	Microbacteriaceae					217:233	family Microbacteriaceae	210:233	family Microbacteriaceae	210:233	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides.					
33524890	7	15	theme	pathogenic	820:829	arg1	species					831:837	plant pathogenic species	814:837	plant pathogenic species of the genus Clavibacter	814:862	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	5	16	with	galactomannan	542:554	arg1	unit					575:578	the repeating unit	561:578	the repeating unit	561:578	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	16	with	galactomannan	542:554	arg1	1 →					641:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	7	17	theme	plant	814:818	arg1	species					831:837	plant pathogenic species	814:837	plant pathogenic species of the genus Clavibacter	814:862	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	4	18	theme	-α-L-Rhap-	475:484	arg1	trisaccharide					441:453	side trisaccharide	436:453	side trisaccharide	436:453	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	18	theme	-α-L-Rhap-	475:484	arg1	1 →					503:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	5	19	theme	1 → 3	595:599	arg1	1 →					641:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	19	theme	1 → 3	595:599	arg1	unit					575:578	the repeating unit	561:578	the repeating unit	561:578	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	4	20	theme	neutral	305:311	arg1	one					298:300	one	298:300	one	298:300	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	20	theme	neutral	305:311	arg1	galactofuranan					328:341	neutral (1 → 6) linked galactofuranan	305:341	neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	305:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	5	21	theme	-α-D-[4,6-S-Pyr	601:615	arg1	1 →					641:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	21	theme	-α-D-[4,6-S-Pyr	601:615	arg1	unit					575:578	the repeating unit	561:578	the repeating unit	561:578	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	3	22	theme	family	210:215	arg1	Actinobacteria					242:255	class Actinobacteria	236:255	class Actinobacteria	236:255	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides.					
33524890	3	22	theme	family	210:215	arg1	Microbacteriaceae					217:233	family Microbacteriaceae	210:233	family Microbacteriaceae	210:233	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides.					
33524890	6	23	theme	wall	655:658	arg1	structures					673:682	The cell wall glycopolymer structures	646:682	The cell wall glycopolymer structures	646:682	The cell wall glycopolymer structures were established by chemical and NMR spectroscopic methods.					
33524890	0	24	from	galactomannan	37:49	arg1	wall					63:66	the cell wall	54:66	the cell wall of Clavibacter michiganensis subsp	54:101	Novel galactofuranan and pyruvylated galactomannan in the cell wall of Clavibacter michiganensis subsp.					
33524890	7	25	from	data	777:780	arg1	composition					799:809	the cell wall composition	785:809	the cell wall composition of plant pathogenic species of the genus Clavibacter	785:862	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	7	26	theme	molecular	898:906	arg1	mechanisms					908:917	the molecular mechanisms	894:917	the molecular mechanisms involved in colonization and infection of plants	894:966	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	4	27	theme	1 → 6	314:318	arg1	one					298:300	one	298:300	one	298:300	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	27	theme	1 → 6	314:318	arg1	galactofuranan					328:341	neutral (1 → 6) linked galactofuranan	305:341	neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	305:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	0	28	theme	Novel	0:4	arg1	galactofuranan					6:19	Novel galactofuranan	0:19	Novel galactofuranan	0:19	Novel galactofuranan and pyruvylated galactomannan in the cell wall of Clavibacter michiganensis subsp.					
33524890	5	29	theme	-Manp-	617:622	arg1	1 →					641:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	29	theme	-Manp-	617:622	arg1	unit					575:578	the repeating unit	561:578	the repeating unit	561:578	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	7	30	theme	wall	794:797	arg1	composition					799:809	the cell wall composition	785:809	the cell wall composition of plant pathogenic species of the genus Clavibacter	785:862	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	0	31	theme	pyruvylated	25:35	arg1	galactomannan					37:49	pyruvylated galactomannan	25:49	pyruvylated galactomannan	25:49	Novel galactofuranan and pyruvylated galactomannan in the cell wall of Clavibacter michiganensis subsp.					
33524890	7	32	theme	genus	846:850	arg1	Clavibacter					852:862	the genus Clavibacter	842:862	the genus Clavibacter	842:862	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	4	33	theme	main	396:399	arg1	chain					401:405	the main chain	392:405	the main chain	392:405	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	5	34	theme	second	512:517	arg1	polymer					519:525	The second polymer	508:525	The second polymer	508:525	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	34	theme	second	512:517	arg1	galactomannan					542:554	pyruvylated galactomannan	530:554	pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	530:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	7	35	theme	cell	789:792	arg1	composition					799:809	the cell wall composition	785:809	the cell wall composition of plant pathogenic species of the genus Clavibacter	785:862	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	6	36	theme	glycopolymer	660:671	arg1	structures					673:682	The cell wall glycopolymer structures	646:682	The cell wall glycopolymer structures	646:682	The cell wall glycopolymer structures were established by chemical and NMR spectroscopic methods.					
33524890	4	37	theme	β-D-GlcpNAc-	456:467	arg1	trisaccharide					441:453	side trisaccharide	436:453	side trisaccharide	436:453	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	37	theme	β-D-GlcpNAc-	456:467	arg1	1 →					503:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	38	from	residue	381:387	arg1	one					298:300	one	298:300	one	298:300	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	38	from	residue	381:387	arg1	chain					401:405	the main chain	392:405	the main chain	392:405	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	38	from	residue	381:387	arg1	galactofuranan					328:341	neutral (1 → 6) linked galactofuranan	305:341	neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	305:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	39	link	linked	321:326	arg1	one					298:300	one	298:300	one	298:300	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	39	link	linked	321:326	arg1	galactofuranan					328:341	neutral (1 → 6) linked galactofuranan	305:341	neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	305:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	6	40	theme	cell	650:653	arg1	structures					673:682	The cell wall glycopolymer structures	646:682	The cell wall glycopolymer structures	646:682	The cell wall glycopolymer structures were established by chemical and NMR spectroscopic methods.					
33524890	7	41	theme	obtained	748:755	arg1	results					757:763	The obtained results	744:763	The obtained results	744:763	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	4	42	theme	second	358:363	arg1	residue					381:387	every second galactofuranose residue	352:387	every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	352:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	0	43	theme	cell	58:61	arg1	wall					63:66	the cell wall	54:66	the cell wall of Clavibacter michiganensis subsp	54:101	Novel galactofuranan and pyruvylated galactomannan in the cell wall of Clavibacter michiganensis subsp.					
33524890	7	44	theme	new	773:775	arg1	data					777:780	new data	773:780	new data on the cell wall composition of plant pathogenic species of the genus Clavibacter	773:862	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	2	45	theme	subsp	175:179	arg1	wall					141:144	The cell wall	132:144	The cell wall of Clavibacter michiganensis subsp.	132:180	The cell wall of Clavibacter michiganensis subsp.					
33524890	4	46	theme	side	436:439	arg1	trisaccharide					441:453	side trisaccharide	436:453	side trisaccharide	436:453	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	46	theme	side	436:439	arg1	1 →					503:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	456:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	5	47	theme	-α-D-Galp-	584:593	arg1	1 →					641:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	47	theme	-α-D-Galp-	584:593	arg1	unit					575:578	the repeating unit	561:578	the repeating unit	561:578	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	48	theme	→3	581:582	arg1	1 →					641:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	48	theme	→3	581:582	arg1	unit					575:578	the repeating unit	561:578	the repeating unit	561:578	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	49	theme	repeating	565:573	arg1	unit					575:578	the repeating unit	561:578	the repeating unit	561:578	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	49	theme	repeating	565:573	arg1	1 →					641:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	7	50	theme	species	831:837	arg1	composition					799:809	the cell wall composition	785:809	the cell wall composition of plant pathogenic species of the genus Clavibacter	785:862	The obtained results provide new data on the cell wall composition of plant pathogenic species of the genus Clavibacter and can promote understanding the molecular mechanisms involved in colonization and infection of plants.					
33524890	4	51	theme	galactofuranose	365:379	arg1	residue					381:387	every second galactofuranose residue	352:387	every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	352:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	3	52	contain	contains	258:265	arg2	polysaccharides					271:285	two polysaccharides	267:285	two polysaccharides	267:285	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides.					
33524890	3	52	contain	contains	258:265	arg1	Ас-1403Т					200:207	michiganensis VKM Ас-1403Т	182:207	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria)	182:256	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides.					
33524890	5	53	theme	pyruvylated	530:540	arg1	polymer					519:525	The second polymer	508:525	The second polymer	508:525	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	53	theme	pyruvylated	530:540	arg1	galactomannan					542:554	pyruvylated galactomannan	530:554	pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	530:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	6	54	dep	chemical	704:711	arg1	methods					735:741	methods	735:741	methods	735:741	The cell wall glycopolymer structures were established by chemical and NMR spectroscopic methods.					
33524890	4	55	theme	linked	321:326	arg1	one					298:300	one	298:300	one	298:300	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	4	55	theme	linked	321:326	arg1	galactofuranan					328:341	neutral (1 → 6) linked galactofuranan	305:341	neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →	305:505	The first one is neutral (1 → 6) linked galactofuranan in which every second galactofuranose residue in the main chain substituted at position 3 by side trisaccharide, β-D-GlcpNAc-(1 → 3)-α-L-Rhap-(1 → 2)-α-D-Fucp-(1 →.					
33524890	5	56	theme	-α-D-Manp-	630:639	arg1	1 →					641:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	→3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →	581:643	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	5	56	theme	-α-D-Manp-	630:639	arg1	unit					575:578	the repeating unit	561:578	the repeating unit	561:578	The second polymer is pyruvylated galactomannan with the repeating unit, →3)-α-D-Galp-(1 → 3)-α-D-[4,6-S-Pyr]-Manp-(1 → 3)-α-D-Manp-(1 →.					
33524890	2	57	theme	cell	136:139	arg1	wall					141:144	The cell wall	132:144	The cell wall of Clavibacter michiganensis subsp.	132:180	The cell wall of Clavibacter michiganensis subsp.					
33524890	3	58	dep	Ас-1403Т	200:207	arg1	Actinobacteria					242:255	class Actinobacteria	236:255	class Actinobacteria	236:255	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides.					
33524890	3	58	dep	Ас-1403Т	200:207	arg1	Microbacteriaceae					217:233	family Microbacteriaceae	210:233	family Microbacteriaceae	210:233	michiganensis VKM Ас-1403Т (family Microbacteriaceae, class Actinobacteria) contains two polysaccharides.					
33766368	0	0	theme	residue	88:94	arg1	algae					82:86	Dunaliella tertiolecta marine green algae	46:86	Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid)	46:144	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	2	1	theme	dominant	445:452	arg1	type					464:467	dominant cellulose type I	445:469	dominant cellulose type I polymorph	445:479	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	1	2	theme	Marine	147:152	arg1	algae					160:164	Marine green algae	147:164	Marine green algae biomass residue (ABR)	147:186	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	3	theme	tertiolecta	222:232	arg1	residue					174:180	Marine green algae biomass residue	147:180	Marine green algae biomass residue (ABR)	147:186	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	3	theme	tertiolecta	222:232	arg1	by-product					197:206	a waste by-product	189:206	a waste by-product of Dunaliella tertiolecta	189:232	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	4	theme	green	154:158	arg1	algae					160:164	Marine green algae	147:164	Marine green algae biomass residue (ABR)	147:186	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	6	5	theme	bio-composite	934:946	arg1	films					948:952	the bio-composite films	930:952	the bio-composite films	930:952	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	0	6	theme	crystallization	99:113	arg1	behaviour					115:123	crystallization behaviour	99:123	crystallization behaviour of poly(lactic acid)	99:144	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	4	7	theme	bio-filler	719:728	arg1	loadings					730:737	varying bio-filler loadings	711:737	varying bio-filler loadings	711:737	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	1	8	theme	left	235:238	arg1	residue					174:180	Marine green algae biomass residue	147:180	Marine green algae biomass residue (ABR)	147:186	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	8	theme	left	235:238	arg1	behind					240:245	left behind	235:245	left behind after the extraction of oil from the algal biomass	235:296	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	6	9	theme	spherulite	1159:1168	arg1	rate					1177:1180	spherulite growth rate	1159:1180	spherulite growth rate	1159:1180	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	4	10	theme	casting	688:694	arg1	technique					696:704	solvent casting technique	680:704	solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA	680:811	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	3	11	from	presence	561:568	arg1	ABR					602:604	the ABR	598:604	the ABR	598:604	ICP-MS elemental analysis confirmed the presence of a variety of minerals in the ABR.					
33766368	1	12	from	biomass	290:296	arg1	extraction					257:266	the extraction	253:266	the extraction of oil from the algal biomass	253:296	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	12	from	biomass	290:296	arg1	oil					271:273	oil	271:273	oil from the algal biomass	271:296	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	13	from	extraction	257:266	arg1	biomass					290:296	the algal biomass	280:296	the algal biomass	280:296	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	4	14	theme	varying	711:717	arg1	loadings					730:737	varying bio-filler loadings	711:737	varying bio-filler loadings	711:737	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	1	15	theme	algae	160:164	arg1	residue					174:180	Marine green algae biomass residue	147:180	Marine green algae biomass residue (ABR)	147:186	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	15	theme	algae	160:164	arg1	by-product					197:206	a waste by-product	189:206	a waste by-product of Dunaliella tertiolecta	189:232	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	15	theme	algae	160:164	arg1	ABR					183:185	ABR	183:185	ABR	183:185	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	15	theme	algae	160:164	arg1	behind					240:245	left behind	235:245	left behind after the extraction of oil from the algal biomass	235:296	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	4	16	theme	crystallization	780:794	arg1	behaviour					796:804	the crystallization behaviour	776:804	the crystallization behaviour of PLA	776:811	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	2	17	contain	had	412:414	arg1	CNCs					407:410	The fabricated sulphuric acid hydrolysed CNCs	366:410	The fabricated sulphuric acid hydrolysed CNCs	366:410	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	2	17	contain	had	412:414	arg2	morphology					428:437	needle-like morphology	416:437	needle-like morphology	416:437	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	1	18	theme	biomass	166:172	arg1	residue					174:180	Marine green algae biomass residue	147:180	Marine green algae biomass residue (ABR)	147:186	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	18	theme	biomass	166:172	arg1	by-product					197:206	a waste by-product	189:206	a waste by-product of Dunaliella tertiolecta	189:232	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	18	theme	biomass	166:172	arg1	ABR					183:185	ABR	183:185	ABR	183:185	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	18	theme	biomass	166:172	arg1	behind					240:245	left behind	235:245	left behind after the extraction of oil from the algal biomass	235:296	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	4	19	theme	PLA	809:811	arg1	behaviour					796:804	the crystallization behaviour	776:804	the crystallization behaviour of PLA	776:811	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	2	20	theme	type	464:467	arg1	polymorph					471:479	dominant cellulose type I polymorph	445:479	dominant cellulose type I polymorph	445:479	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	4	21	theme	ABR	614:616	arg1	/PLA					624:627	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films	607:659	/PLA	624:627	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	2	22	theme	hydrolysed	396:405	arg1	CNCs					407:410	The fabricated sulphuric acid hydrolysed CNCs	366:410	The fabricated sulphuric acid hydrolysed CNCs	366:410	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	3	23	theme	ICP-MS	521:526	arg1	analysis					538:545	ICP-MS elemental analysis	521:545	ICP-MS elemental analysis	521:545	ICP-MS elemental analysis confirmed the presence of a variety of minerals in the ABR.					
33766368	6	24	theme	films	948:952	arg1	nucleating					886:895	nucleating	886:895	nucleating	886:895	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	6	24	theme	films	948:952	arg1	behaviour					917:925	crystallization behaviour	901:925	crystallization behaviour	901:925	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	4	25	theme	Washed	607:612	arg1	/PLA					624:627	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films	607:659	/PLA	624:627	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	0	26	theme	nanocrystals	20:31	arg1	Effect					0:5	Effect	0:5	Effect of cellulose nanocrystals	0:31	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	6	27	theme	crystallization	901:915	arg1	behaviour					917:925	crystallization behaviour	901:925	crystallization behaviour	901:925	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	4	28	theme	solvent	680:686	arg1	technique					696:704	solvent casting technique	680:704	solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA	680:811	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	2	29	theme	fabricated	370:379	arg1	CNCs					407:410	The fabricated sulphuric acid hydrolysed CNCs	366:410	The fabricated sulphuric acid hydrolysed CNCs	366:410	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	0	30	theme	cellulose	10:18	arg1	nanocrystals					20:31	cellulose nanocrystals	10:31	cellulose nanocrystals	10:31	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	4	31	with	technique	696:704	arg1	loadings					730:737	varying bio-filler loadings	711:737	varying bio-filler loadings	711:737	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	0	32	theme	poly	128:131	arg1	behaviour					115:123	crystallization behaviour	99:123	crystallization behaviour of poly(lactic acid)	99:144	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	1	33	theme	cellulose	335:343	arg1	CNCs					359:362	CNCs	359:362	CNCs	359:362	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	33	theme	cellulose	335:343	arg1	nanocrystals					345:356	cellulose nanocrystals	335:356	cellulose nanocrystals (CNCs)	335:363	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	6	34	theme	cold	1079:1082	arg1	temperature					1100:1110	cold crystallization temperature	1079:1110	cold crystallization temperature	1079:1110	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	2	35	theme	needle-like	416:426	arg1	morphology					428:437	needle-like morphology	416:437	needle-like morphology	416:437	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	2	36	theme	crystallinity	492:504	arg1	index					506:510	a high crystallinity index	485:510	a high crystallinity index of 89 %	485:518	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	1	37	theme	nanocrystals	345:356	arg1	fabrication					320:330	the fabrication	316:330	the fabrication of cellulose nanocrystals (CNCs)	316:363	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	6	38	theme	better	1018:1023	arg1	effectiveness					1025:1037	better effectiveness	1018:1037	better effectiveness of CNCs	1018:1045	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	1	39	theme	oil	271:273	arg1	extraction					257:266	the extraction	253:266	the extraction of oil from the algal biomass	253:296	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	0	40	theme	lactic	133:138	arg1	poly					128:131	poly	128:131	poly(lactic acid)	128:144	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	0	40	theme	lactic	133:138	arg1	acid					140:143	lactic acid	133:143	lactic acid	133:143	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	2	41	theme	acid	391:394	arg1	CNCs					407:410	The fabricated sulphuric acid hydrolysed CNCs	366:410	The fabricated sulphuric acid hydrolysed CNCs	366:410	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	2	42	theme	high	487:490	arg1	index					506:510	a high crystallinity index	485:510	a high crystallinity index of 89 %	485:518	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	0	43	theme	tertiolecta	57:67	arg1	algae					82:86	Dunaliella tertiolecta marine green algae	46:86	Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid)	46:144	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	1	44	used	utilized	303:310	arg2	behind					240:245	left behind	235:245	left behind after the extraction of oil from the algal biomass	235:296	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	44	used	utilized	303:310	arg2	ABR					183:185	ABR	183:185	ABR	183:185	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	44	used	utilized	303:310	arg2	by-product					197:206	a waste by-product	189:206	a waste by-product of Dunaliella tertiolecta	189:232	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	44	used	utilized	303:310	arg2	residue					174:180	Marine green algae biomass residue	147:180	Marine green algae biomass residue (ABR)	147:186	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	2	45	theme	cellulose	454:462	arg1	type					464:467	dominant cellulose type I	445:469	dominant cellulose type I polymorph	445:479	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	3	46	theme	elemental	528:536	arg1	analysis					538:545	ICP-MS elemental analysis	521:545	ICP-MS elemental analysis	521:545	ICP-MS elemental analysis confirmed the presence of a variety of minerals in the ABR.					
33766368	4	47	theme	CNC/PLA	633:639	arg1	films					655:659	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films	607:659	films	655:659	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	0	48	theme	Dunaliella	46:55	arg1	algae					82:86	Dunaliella tertiolecta marine green algae	46:86	Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid)	46:144	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	3	49	theme	minerals	586:593	arg1	minerals					586:593	minerals	586:593	minerals	586:593	ICP-MS elemental analysis confirmed the presence of a variety of minerals in the ABR.					
33766368	3	49	theme	minerals	586:593	arg1	variety					575:581	a variety	573:581	a variety of minerals	573:593	ICP-MS elemental analysis confirmed the presence of a variety of minerals in the ABR.					
33766368	6	50	dep	nucleating	886:895	arg1	The					882:884	The	882:884	The	882:884	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	4	51	from	effectiveness	759:771	arg1	behaviour					796:804	the crystallization behaviour	776:804	the crystallization behaviour of PLA	776:811	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	1	52	theme	algal	284:288	arg1	biomass					290:296	the algal biomass	280:296	the algal biomass	280:296	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	2	53	theme	%	518:518	arg1	index					506:510	a high crystallinity index	485:510	a high crystallinity index of 89 %	485:518	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	2	53	theme	%	518:518	arg1	polymorph					471:479	dominant cellulose type I polymorph	445:479	dominant cellulose type I polymorph	445:479	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	6	54	theme	noteworthy	1117:1126	arg1	increment					1128:1136	noteworthy increment	1117:1136	noteworthy increment	1117:1136	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	6	55	theme	growth	1170:1175	arg1	rate					1177:1180	spherulite growth rate	1159:1180	spherulite growth rate	1159:1180	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	6	56	from	reduction	1066:1074	arg1	temperature					1100:1110	cold crystallization temperature	1079:1110	cold crystallization temperature	1079:1110	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	6	57	theme	significant	1054:1064	arg1	reduction					1066:1074	a significant reduction	1052:1074	a significant reduction in cold crystallization temperature	1052:1110	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	6	58	theme	crystallization	1084:1098	arg1	temperature					1100:1110	cold crystallization temperature	1079:1110	cold crystallization temperature	1079:1110	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	1	59	theme	waste	191:195	arg1	residue					174:180	Marine green algae biomass residue	147:180	Marine green algae biomass residue (ABR)	147:186	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	1	59	theme	waste	191:195	arg1	by-product					197:206	a waste by-product	189:206	a waste by-product of Dunaliella tertiolecta	189:232	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33766368	3	60	theme	variety	575:581	arg1	presence					561:568	the presence	557:568	the presence of a variety of minerals in the ABR	557:604	ICP-MS elemental analysis confirmed the presence of a variety of minerals in the ABR.					
33766368	4	61	theme	bio-composite	641:653	arg1	films					655:659	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films	607:659	films	655:659	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	0	62	theme	green	76:80	arg1	algae					82:86	Dunaliella tertiolecta marine green algae	46:86	Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid)	46:144	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	5	63	used	used	844:847	arg2	FTIR					821:824	FTIR	821:824	FTIR	821:824	FESEM, FTIR, XRD and TGA were used to characterize the bio-fillers.					
33766368	5	63	used	used	844:847	arg2	FESEM					814:818	FESEM	814:818	FESEM	814:818	FESEM, FTIR, XRD and TGA were used to characterize the bio-fillers.					
33766368	5	63	used	used	844:847	arg2	TGA					835:837	TGA	835:837	TGA	835:837	FESEM, FTIR, XRD and TGA were used to characterize the bio-fillers.					
33766368	5	63	used	used	844:847	arg2	XRD					827:829	XRD	827:829	XRD	827:829	FESEM, FTIR, XRD and TGA were used to characterize the bio-fillers.					
33766368	6	64	theme	POM	989:991	arg1	analysis					993:1000	POM analysis	989:1000	POM analysis	989:1000	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	4	65	theme	WABR	619:622	arg1	/PLA					624:627	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films	607:659	/PLA	624:627	Washed ABR (WABR)/PLA and CNC/PLA bio-composite films were developed via solvent casting technique with varying bio-filler loadings for comparing their effectiveness on the crystallization behaviour of PLA.					
33766368	3	66	attach	presence	561:568	arg2	variety					575:581	a variety	573:581	a variety of minerals	573:593	ICP-MS elemental analysis confirmed the presence of a variety of minerals in the ABR.					
33766368	3	66	attach	presence	561:568	arg2	minerals					586:593	minerals	586:593	minerals	586:593	ICP-MS elemental analysis confirmed the presence of a variety of minerals in the ABR.					
33766368	3	66	attach	presence	561:568	arg1	ABR					602:604	the ABR	598:604	the ABR	598:604	ICP-MS elemental analysis confirmed the presence of a variety of minerals in the ABR.					
33766368	0	67	theme	marine	69:74	arg1	algae					82:86	Dunaliella tertiolecta marine green algae	46:86	Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid)	46:144	Effect of cellulose nanocrystals derived from Dunaliella tertiolecta marine green algae residue on crystallization behaviour of poly(lactic acid).					
33766368	2	68	theme	sulphuric	381:389	arg1	CNCs					407:410	The fabricated sulphuric acid hydrolysed CNCs	366:410	The fabricated sulphuric acid hydrolysed CNCs	366:410	The fabricated sulphuric acid hydrolysed CNCs had needle-like morphology, with dominant cellulose type I polymorph and a high crystallinity index of 89 %.					
33766368	6	69	theme	CNCs	1042:1045	arg1	effectiveness					1025:1037	better effectiveness	1018:1037	better effectiveness of CNCs	1018:1045	The nucleating and crystallization behaviour of the bio-composite films were confirmed using DSC, SAXS and POM analysis which indicated better effectiveness of CNCs with a significant reduction in cold crystallization temperature, and noteworthy increment in crystallinity and spherulite growth rate.					
33766368	1	70	theme	Dunaliella	211:220	arg1	tertiolecta					222:232	Dunaliella tertiolecta	211:232	Dunaliella tertiolecta	211:232	Marine green algae biomass residue (ABR), a waste by-product of Dunaliella tertiolecta, left behind after the extraction of oil from the algal biomass, was utilized for the fabrication of cellulose nanocrystals (CNCs).					
33675828	6	0	theme	excellent	1038:1046	arg1	capacity					1057:1064	the above excellent swelling capacity	1028:1064	the above excellent swelling capacity	1028:1064	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	4	1	theme	swelling	592:599	arg1	ratio					601:605	The swelling ratio	588:605	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution	588:690	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	4	1	theme	swelling	592:599	arg1	410.8					696:700	410.8	696:700	410.8	696:700	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	5	2	theme	soil	918:921	arg1	efficiency					939:948	the soil water retention efficiency	914:948	the soil water retention efficiency	914:948	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	6	3	theme	soil	1219:1222	arg1	regulator					1224:1232	soil regulator	1219:1232	soil regulator	1219:1232	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	6	4	theme	swelling	1048:1055	arg1	capacity					1057:1064	the above excellent swelling capacity	1028:1064	the above excellent swelling capacity	1028:1064	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	4	5	theme	NaCl	678:681	arg1	solution					683:690	0.9 wt% NaCl solution	670:690	0.9 wt% NaCl solution	670:690	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	3	6	theme	hydrogen	544:551	arg1	bond					553:556	hydrogen bond	544:556	hydrogen bond	544:556	The result indicated that the SC interacted with the polymeric network by hydrogen bond or electrostatic interaction.					
33675828	2	7	dep	transform	357:365	arg1	infrared					367:374	infrared	367:374	transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM)	357:467	The obtained products were characterized in combination with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).					
33675828	2	8	with	combination	332:342	arg1	Fourier					349:355	Fourier	349:355	Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM)	349:467	The obtained products were characterized in combination with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).					
33675828	4	9	from	ratio	601:605	arg1	solution					683:690	0.9 wt% NaCl solution	670:690	0.9 wt% NaCl solution	670:690	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	4	9	from	ratio	601:605	arg1	water					660:664	distilled water	650:664	distilled water	650:664	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	5	10	theme	retention	929:937	arg1	efficiency					939:948	the soil water retention efficiency	914:948	the soil water retention efficiency	914:948	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	2	11	theme	obtained	292:299	arg1	products					301:308	The obtained products	288:308	The obtained products	288:308	The obtained products were characterized in combination with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).					
33675828	1	12	theme	SC	284:285	arg1	presence					272:279	the presence	268:279	the presence of SC	268:285	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	6	13	theme	retaining	1200:1208	arg1	agent					1210:1214	a water retaining agent	1192:1214	a water retaining agent	1192:1214	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	4	14	theme	distilled	650:658	arg1	water					660:664	distilled water	650:664	distilled water	650:664	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	5	15	theme	certain	858:864	arg1	XG-g-PAA/SC					876:886	XG-g-PAA/SC	876:886	XG-g-PAA/SC	876:886	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	5	15	theme	certain	858:864	arg1	amount					866:871	a certain amount	856:871	a certain amount of XG-g-PAA/SC	856:886	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	6	16	theme	water	1194:1198	arg1	agent					1210:1214	a water retaining agent	1192:1214	a water retaining agent	1192:1214	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	6	17	theme	above	1032:1036	arg1	capacity					1057:1064	the above excellent swelling capacity	1028:1064	the above excellent swelling capacity	1028:1064	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	5	18	theme	blank	791:795	arg1	sample					797:802	the blank sample	787:802	the blank sample (only containing soil)	787:825	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	5	18	theme	blank	791:795	arg1	soil					821:824	only containing soil	805:824	only containing soil	805:824	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	6	19	theme	promoting	1107:1115	arg1	performance					1117:1127	plant growth promoting performance	1094:1127	plant growth promoting performance	1094:1127	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	2	20	dep	Fourier	349:355	arg1	transform					357:365	transform	357:365	transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM)	357:467	The obtained products were characterized in combination with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).					
33675828	2	21	theme	thermogravimetric	397:413	arg1	analysis					415:422	thermogravimetric analysis	397:422	thermogravimetric analysis (TGA)	397:428	The obtained products were characterized in combination with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).					
33675828	2	21	theme	thermogravimetric	397:413	arg1	TGA					425:427	TGA	425:427	TGA	425:427	The obtained products were characterized in combination with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).					
33675828	3	22	theme	electrostatic	561:573	arg1	interaction					575:585	electrostatic interaction	561:585	electrostatic interaction	561:585	The result indicated that the SC interacted with the polymeric network by hydrogen bond or electrostatic interaction.					
33675828	6	23	theme	growth	1100:1105	arg1	performance					1117:1127	plant growth promoting performance	1094:1127	plant growth promoting performance	1094:1127	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	4	24	theme	polymerization	733:746	arg1	conditions					748:757	the polymerization conditions	729:757	the polymerization conditions	729:757	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	2	25	theme	scanning	434:441	arg1	SEM					464:466	SEM	464:466	SEM	464:466	The obtained products were characterized in combination with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).					
33675828	2	25	theme	scanning	434:441	arg1	microscopy					452:461	scanning electron microscopy	434:461	scanning electron microscopy (SEM)	434:467	The obtained products were characterized in combination with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).					
33675828	2	26	theme	electron	443:450	arg1	SEM					464:466	SEM	464:466	SEM	464:466	The obtained products were characterized in combination with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).					
33675828	2	26	theme	electron	443:450	arg1	microscopy					452:461	scanning electron microscopy	434:461	scanning electron microscopy (SEM)	434:467	The obtained products were characterized in combination with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM).					
33675828	1	27	theme	eco-friendly	107:118	arg1	acid/semi-coke					175:188	xanthan gum-g-polyacrylic acid/semi-coke	149:188	xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC)	149:202	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	1	27	theme	eco-friendly	107:118	arg1	composites					135:144	An eco-friendly superabsorbent composites	104:144	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC)	104:202	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	4	28	theme	%	676:676	arg1	solution					683:690	0.9 wt% NaCl solution	670:690	0.9 wt% NaCl solution	670:690	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	0	29	theme	superabsorbent	42:55	arg1	composites					57:66	eco-friendly superabsorbent composites	29:66	eco-friendly superabsorbent composites	29:66	Synthesis and application of eco-friendly superabsorbent composites based on xanthan gum and semi-coke.					
33675828	1	30	theme	polyacrylic	236:246	arg1	acid					248:251	polyacrylic acid	236:251	polyacrylic acid	236:251	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	0	31	theme	eco-friendly	29:40	arg1	composites					57:66	eco-friendly superabsorbent composites	29:66	eco-friendly superabsorbent composites	29:66	Synthesis and application of eco-friendly superabsorbent composites based on xanthan gum and semi-coke.					
33675828	5	32	located	found	838:842	arg2	it					828:829	it	828:829	it	828:829	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	5	32	located	found	838:842	arg1	addition					763:770	addition	763:770	addition	763:770	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	1	33	theme	acid	248:251	arg1	grafting					224:231	grafting	224:231	grafting of polyacrylic acid onto the XG in the presence of SC	224:285	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	1	34	from	XG	262:263	arg1	presence					272:279	the presence	268:279	the presence of SC	268:285	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	1	35	theme	superabsorbent	120:133	arg1	acid/semi-coke					175:188	xanthan gum-g-polyacrylic acid/semi-coke	149:188	xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC)	149:202	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	1	35	theme	superabsorbent	120:133	arg1	composites					135:144	An eco-friendly superabsorbent composites	104:144	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC)	104:202	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	0	36	theme	composites	57:66	arg1	application					14:24	application	14:24	application	14:24	Synthesis and application of eco-friendly superabsorbent composites based on xanthan gum and semi-coke.					
33675828	0	36	theme	composites	57:66	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and application of eco-friendly superabsorbent composites based on xanthan gum and semi-coke.					
33675828	1	37	theme	acid/semi-coke	175:188	arg1	acid/semi-coke					175:188	xanthan gum-g-polyacrylic acid/semi-coke	149:188	xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC)	149:202	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	1	37	theme	acid/semi-coke	175:188	arg1	composites					135:144	An eco-friendly superabsorbent composites	104:144	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC)	104:202	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	6	38	theme	holding	1073:1079	arg1	capacity					1081:1088	water holding capacity	1067:1088	water holding capacity	1067:1088	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	6	39	theme	plant	1238:1242	arg1	growth					1244:1249	plant growth	1238:1249	plant growth	1238:1249	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	5	40	theme	plant	997:1001	arg1	experiment					1007:1016	plant pot experiment	997:1016	plant pot experiment	997:1016	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	4	41	theme	0.9 wt	670:675	arg1	solution					683:690	0.9 wt% NaCl solution	670:690	0.9 wt% NaCl solution	670:690	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	5	42	theme	XG-g-PAA/SC	876:886	arg1	XG-g-PAA/SC					876:886	XG-g-PAA/SC	876:886	XG-g-PAA/SC	876:886	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	5	42	theme	XG-g-PAA/SC	876:886	arg1	amount					866:871	a certain amount	856:871	a certain amount of XG-g-PAA/SC	856:886	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	6	43	theme	plant	1094:1098	arg1	performance					1117:1127	plant growth promoting performance	1094:1127	plant growth promoting performance	1094:1127	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	4	44	theme	best	614:617	arg1	%					644:644	15 wt%	639:644	15 wt%	639:644	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	4	44	theme	best	614:617	arg1	XG-g-PAA/SC					626:636	the best sample XG-g-PAA/SC	610:636	the best sample XG-g-PAA/SC (15 wt%)	610:645	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	5	45	theme	pot	1003:1005	arg1	experiment					1007:1016	plant pot experiment	997:1016	plant pot experiment	997:1016	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	5	46	theme	water	923:927	arg1	efficiency					939:948	the soil water retention efficiency	914:948	the soil water retention efficiency	914:948	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	1	47	theme	xanthan	149:155	arg1	acid/semi-coke					175:188	xanthan gum-g-polyacrylic acid/semi-coke	149:188	xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC)	149:202	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	1	47	theme	xanthan	149:155	arg1	XG-g-PAA/SC					191:201	XG-g-PAA/SC	191:201	XG-g-PAA/SC	191:201	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	6	48	theme	water	1067:1071	arg1	capacity					1081:1088	water holding capacity	1067:1088	water holding capacity	1067:1088	Based on the above excellent swelling capacity, water holding capacity and plant growth promoting performance, it can be inferred that the XG-g-PAA/SC is expected to become a water retaining agent or soil regulator for plant growth.					
33675828	0	49	theme	xanthan	77:83	arg1	gum					85:87	xanthan gum	77:87	xanthan gum	77:87	Synthesis and application of eco-friendly superabsorbent composites based on xanthan gum and semi-coke.					
33675828	5	50	theme	experiment	1007:1016	arg1	results					986:992	the results	982:992	the results of plant pot experiment	982:1016	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	5	51	theme	containing	810:819	arg1	sample					797:802	the blank sample	787:802	the blank sample (only containing soil)	787:825	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	5	51	theme	containing	810:819	arg1	soil					821:824	only containing soil	805:824	only containing soil	805:824	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	1	52	theme	gum-g-polyacrylic	157:173	arg1	acid/semi-coke					175:188	xanthan gum-g-polyacrylic acid/semi-coke	149:188	xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC)	149:202	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	1	52	theme	gum-g-polyacrylic	157:173	arg1	XG-g-PAA/SC					191:201	XG-g-PAA/SC	191:201	XG-g-PAA/SC	191:201	An eco-friendly superabsorbent composites of xanthan gum-g-polyacrylic acid/semi-coke (XG-g-PAA/SC) were fabricated via grafting of polyacrylic acid onto the XG in the presence of SC.					
33675828	4	53	theme	XG-g-PAA/SC	626:636	arg1	ratio					601:605	The swelling ratio	588:605	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution	588:690	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	4	53	theme	XG-g-PAA/SC	626:636	arg1	410.8					696:700	410.8	696:700	410.8	696:700	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	5	54	theme	only	805:808	arg1	sample					797:802	the blank sample	787:802	the blank sample (only containing soil)	787:825	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	5	54	theme	only	805:808	arg1	soil					821:824	only containing soil	805:824	only containing soil	805:824	In addition, compared with the blank sample (only containing soil), it can be found that adding a certain amount of XG-g-PAA/SC can significantly improve the soil water retention efficiency, which can be further proved by the results of plant pot experiment.					
33675828	3	55	theme	polymeric	523:531	arg1	network					533:539	the polymeric network	519:539	the polymeric network	519:539	The result indicated that the SC interacted with the polymeric network by hydrogen bond or electrostatic interaction.					
33675828	4	56	theme	sample	619:624	arg1	%					644:644	15 wt%	639:644	15 wt%	639:644	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
33675828	4	56	theme	sample	619:624	arg1	XG-g-PAA/SC					626:636	the best sample XG-g-PAA/SC	610:636	the best sample XG-g-PAA/SC (15 wt%)	610:645	The swelling ratio of the best sample XG-g-PAA/SC (15 wt%) in distilled water and 0.9 wt% NaCl solution was 410.8 and 61.5 g/g by optimizing the polymerization conditions.					
32429136	0	0	from	Effect	14:19	arg1	Pretreatment					55:66	the Pretreatment	51:66	the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids	51:129	Exploring the Effect of Water Content and Anion on the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids.					
32429136	2	1	dep	lignin	385:390	arg1	the					381:383	the	381:383	the	381:383	The performance was evaluated by observing the lignin and hemicellulose removal, as well as enzymatic saccharification and lignin yield.					
32429136	5	2	theme	saccharification	1021:1036	arg1	yield					1038:1042	saccharification yield	1021:1042	saccharification yield	1021:1042	The methanesulfonate ionic liquid did not introduce significant fractionation or enhancement of saccharification yield under the conditions used.					
32429136	6	3	theme	solvent	1140:1146	arg1	acidity					1148:1154	solvent acidity	1140:1154	solvent acidity	1140:1154	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	4	4	theme	higher	868:873	arg1	contents					881:888	higher water contents	868:888	higher water contents	868:888	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	1	5	with	pretreatment	149:160	arg1	[EMim					241:245	[EMim	241:245	[EMim][OAc]	241:251	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	5	with	pretreatment	149:160	arg1	[EMim					273:277	[EMim][HSO4]	273:284	[EMim][HSO4]	273:284	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	5	with	pretreatment	149:160	arg1	liquids					232:238	three different 1-ethyl-3-methylimidazolium ionic liquids	182:238	three different 1-ethyl-3-methylimidazolium ionic liquids	182:238	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	5	with	pretreatment	149:160	arg1	[EMim					254:258	[EMim][MeSO3]	254:266	[EMim][MeSO3]	254:266	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	6	6	theme	content	1237:1243	arg1	selections					1245:1254	anion and water content selections	1221:1254	anion and water content selections for different applications	1221:1281	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	1	7	dep	[EMim	241:245	arg1	[OAc					247:250	[OAc	247:250	[OAc	247:250	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	6	8	theme	ionic	1178:1182	arg1	pretreatment					1191:1202	ionic liquid pretreatment	1178:1202	ionic liquid pretreatment	1178:1202	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	3	9	theme	ionic	573:577	arg1	delignification					597:611	the hydrogen sulfate ionic liquid performing delignification	552:611	the hydrogen sulfate ionic liquid performing delignification	552:611	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	0	10	with	Pretreatment	55:66	arg1	Liquids					123:129	Three 1-Ethyl-3-methylimidazolium Ionic Liquids	83:129	Three 1-Ethyl-3-methylimidazolium Ionic Liquids	83:129	Exploring the Effect of Water Content and Anion on the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids.					
32429136	6	11	theme	water	1161:1165	arg1	content					1167:1173	water content	1161:1173	water content	1161:1173	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	0	12	theme	Poplar	71:76	arg1	Pretreatment					55:66	the Pretreatment	51:66	the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids	51:129	Exploring the Effect of Water Content and Anion on the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids.					
32429136	1	13	theme	water	298:302	arg1	contents					304:311	water contents	298:311	water contents	298:311	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	4	14	theme	sulfate	827:833	arg1	hydrogen					818:825	the hydrogen sulfate ionic liquid	814:846	the hydrogen sulfate ionic liquid	814:846	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	2	15	theme	enzymatic	430:438	arg1	saccharification					440:455	enzymatic saccharification	430:455	enzymatic saccharification	430:455	The performance was evaluated by observing the lignin and hemicellulose removal, as well as enzymatic saccharification and lignin yield.					
32429136	6	16	theme	water	1231:1235	arg1	content					1237:1243	water content	1231:1243	water content	1231:1243	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	6	17	theme	different	1260:1268	arg1	applications					1270:1281	different applications	1260:1281	different applications	1260:1281	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	4	18	theme	recoverable	905:915	arg1	lignin					917:922	a recoverable lignin	903:922	a recoverable lignin	903:922	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	2	19	theme	hemicellulose	396:408	arg1	removal					410:416	hemicellulose removal	396:416	hemicellulose removal	396:416	The performance was evaluated by observing the lignin and hemicellulose removal, as well as enzymatic saccharification and lignin yield.					
32429136	5	20	theme	ionic	946:950	arg1	methanesulfonate					929:944	The methanesulfonate ionic liquid	925:957	The methanesulfonate ionic liquid	925:957	The methanesulfonate ionic liquid did not introduce significant fractionation or enhancement of saccharification yield under the conditions used.					
32429136	1	21	theme	poplar	165:170	arg1	wood					172:175	poplar wood	165:175	poplar wood	165:175	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	3	22	theme	other	668:672	arg1	solvents					674:681	the other solvents	664:681	the other solvents across the investigated water content range	664:725	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	6	23	theme	anion	1221:1225	arg1	selections					1245:1254	anion and water content selections	1221:1254	anion and water content selections for different applications	1221:1281	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	3	24	theme	pretreatment	492:503	arg1	mechanism					479:487	The mechanism	475:487	The mechanism of pretreatment	475:503	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	1	25	theme	wood	172:175	arg1	pretreatment					149:160	the pretreatment	145:160	the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4],	145:285	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	3	26	theme	water	707:711	arg1	content					713:719	water content	707:719	the investigated water content range	690:725	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	5	27	theme	significant	977:987	arg1	fractionation					989:1001	significant fractionation	977:1001	significant fractionation	977:1001	The methanesulfonate ionic liquid did not introduce significant fractionation or enhancement of saccharification yield under the conditions used.					
32429136	3	28	theme	content	713:719	arg1	range					721:725	the investigated water content range	690:725	the investigated water content range	690:725	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	4	29	theme	water	875:879	arg1	contents					881:888	higher water contents	868:888	higher water contents	868:888	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	6	30	theme	bonding	1131:1137	arg1	roles					1107:1111	the roles	1103:1111	the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment	1103:1202	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	3	31	theme	ionic	524:528	arg1	liquids					530:536	the ionic liquids	520:536	the ionic liquids studied	520:544	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	0	32	theme	Ionic	117:121	arg1	Liquids					123:129	Three 1-Ethyl-3-methylimidazolium Ionic Liquids	83:129	Three 1-Ethyl-3-methylimidazolium Ionic Liquids	83:129	Exploring the Effect of Water Content and Anion on the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids.					
32429136	1	33	theme	wt	323:324	arg1	%					325:325	0-40 wt%	318:325	0-40 wt%	318:325	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	6	34	theme	hydrogen	1122:1129	arg1	bonding					1131:1137	anion hydrogen bonding	1116:1137	anion hydrogen bonding	1116:1137	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	1	35	theme	different	188:196	arg1	[EMim					241:245	[EMim	241:245	[EMim][OAc]	241:251	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	35	theme	different	188:196	arg1	[EMim					273:277	[EMim][HSO4]	273:284	[EMim][HSO4]	273:284	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	35	theme	different	188:196	arg1	liquids					232:238	three different 1-ethyl-3-methylimidazolium ionic liquids	182:238	three different 1-ethyl-3-methylimidazolium ionic liquids	182:238	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	35	theme	different	188:196	arg1	[EMim					254:258	[EMim][MeSO3]	254:266	[EMim][MeSO3]	254:266	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	0	36	theme	Content	30:36	arg1	Effect					14:19	the Effect	10:19	the Effect of Water Content and Anion on the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids	10:129	Exploring the Effect of Water Content and Anion on the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids.					
32429136	3	37	theme	investigated	694:705	arg1	range					721:725	the investigated water content range	690:725	the investigated water content range	690:725	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	3	38	theme	liquid	579:584	arg1	delignification					597:611	the hydrogen sulfate ionic liquid performing delignification	552:611	the hydrogen sulfate ionic liquid performing delignification	552:611	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	0	39	theme	1-Ethyl-3-methylimidazolium	89:115	arg1	Liquids					123:129	Three 1-Ethyl-3-methylimidazolium Ionic Liquids	83:129	Three 1-Ethyl-3-methylimidazolium Ionic Liquids	83:129	Exploring the Effect of Water Content and Anion on the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids.					
32429136	1	40	theme	1-ethyl-3-methylimidazolium	198:224	arg1	[EMim					241:245	[EMim	241:245	[EMim][OAc]	241:251	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	40	theme	1-ethyl-3-methylimidazolium	198:224	arg1	[EMim					273:277	[EMim][HSO4]	273:284	[EMim][HSO4]	273:284	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	40	theme	1-ethyl-3-methylimidazolium	198:224	arg1	liquids					232:238	three different 1-ethyl-3-methylimidazolium ionic liquids	182:238	three different 1-ethyl-3-methylimidazolium ionic liquids	182:238	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	40	theme	1-ethyl-3-methylimidazolium	198:224	arg1	[EMim					254:258	[EMim][MeSO3]	254:266	[EMim][MeSO3]	254:266	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	0	41	theme	Water	24:28	arg1	Content					30:36	Water Content	24:36	Water Content	24:36	Exploring the Effect of Water Content and Anion on the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids.					
32429136	2	42	theme	lignin	461:466	arg1	yield					468:472	lignin yield	461:472	lignin yield	461:472	The performance was evaluated by observing the lignin and hemicellulose removal, as well as enzymatic saccharification and lignin yield.					
32429136	3	43	theme	hydrogen	556:563	arg1	delignification					597:611	the hydrogen sulfate ionic liquid performing delignification	552:611	the hydrogen sulfate ionic liquid performing delignification	552:611	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	4	44	theme	glucose	771:777	arg1	yield					779:783	superior glucose yield	762:783	superior glucose yield	762:783	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	6	45	from	roles	1107:1111	arg1	pretreatment					1191:1202	ionic liquid pretreatment	1178:1202	ionic liquid pretreatment	1178:1202	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	1	46	theme	ionic	226:230	arg1	[EMim					241:245	[EMim	241:245	[EMim][OAc]	241:251	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	46	theme	ionic	226:230	arg1	[EMim					273:277	[EMim][HSO4]	273:284	[EMim][HSO4]	273:284	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	46	theme	ionic	226:230	arg1	liquids					232:238	three different 1-ethyl-3-methylimidazolium ionic liquids	182:238	three different 1-ethyl-3-methylimidazolium ionic liquids	182:238	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	1	46	theme	ionic	226:230	arg1	[EMim					254:258	[EMim][MeSO3]	254:266	[EMim][MeSO3]	254:266	We report on the pretreatment of poplar wood with three different 1-ethyl-3-methylimidazolium ionic liquids, [EMim][OAc], [EMim][MeSO3], and [EMim][HSO4], at varying water contents from 0-40 wt% at 100 °C.					
32429136	0	47	theme	Anion	42:46	arg1	Effect					14:19	the Effect	10:19	the Effect of Water Content and Anion on the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids	10:129	Exploring the Effect of Water Content and Anion on the Pretreatment of Poplar with Three 1-Ethyl-3-methylimidazolium Ionic Liquids.					
32429136	3	48	theme	sulfate	565:571	arg1	delignification					597:611	the hydrogen sulfate ionic liquid performing delignification	552:611	the hydrogen sulfate ionic liquid performing delignification	552:611	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	4	49	theme	low	788:790	arg1	contents					798:805	low water contents	788:805	low water contents	788:805	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	6	50	theme	anion	1116:1120	arg1	bonding					1131:1137	anion hydrogen bonding	1116:1137	anion hydrogen bonding	1116:1137	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	4	51	theme	liquid	841:846	arg1	hydrogen					818:825	the hydrogen sulfate ionic liquid	814:846	the hydrogen sulfate ionic liquid	814:846	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	4	52	theme	water	792:796	arg1	contents					798:805	low water contents	788:805	low water contents	788:805	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	4	53	theme	ionic	835:839	arg1	hydrogen					818:825	the hydrogen sulfate ionic liquid	814:846	the hydrogen sulfate ionic liquid	814:846	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	3	54	theme	hemicellulose	617:629	arg1	hydrolysis					631:640	hemicellulose hydrolysis	617:640	hemicellulose hydrolysis	617:640	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	4	55	theme	liquid	746:751	arg1	acetate					732:738	The acetate ionic liquid	728:751	The acetate ionic liquid	728:751	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	6	56	theme	acidity	1148:1154	arg1	roles					1107:1111	the roles	1103:1111	the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment	1103:1202	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	3	57	theme	performing	586:595	arg1	delignification					597:611	the hydrogen sulfate ionic liquid performing delignification	552:611	the hydrogen sulfate ionic liquid performing delignification	552:611	The mechanism of pretreatment varied between the ionic liquids studied, with the hydrogen sulfate ionic liquid performing delignification and hemicellulose hydrolysis more effectively than the other solvents across the investigated water content range.					
32429136	4	58	theme	ionic	740:744	arg1	acetate					732:738	The acetate ionic liquid	728:751	The acetate ionic liquid	728:751	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	5	59	theme	yield	1038:1042	arg1	fractionation					989:1001	significant fractionation	977:1001	significant fractionation	977:1001	The methanesulfonate ionic liquid did not introduce significant fractionation or enhancement of saccharification yield under the conditions used.					
32429136	5	59	theme	yield	1038:1042	arg1	enhancement					1006:1016	enhancement	1006:1016	enhancement of saccharification yield	1006:1042	The methanesulfonate ionic liquid did not introduce significant fractionation or enhancement of saccharification yield under the conditions used.					
32429136	4	60	theme	superior	762:769	arg1	yield					779:783	superior glucose yield	762:783	superior glucose yield	762:783	The acetate ionic liquid produced superior glucose yield at low water contents, while the hydrogen sulfate ionic liquid performed better at higher water contents and produced a recoverable lignin.					
32429136	5	61	theme	liquid	952:957	arg1	methanesulfonate					929:944	The methanesulfonate ionic liquid	925:957	The methanesulfonate ionic liquid	925:957	The methanesulfonate ionic liquid did not introduce significant fractionation or enhancement of saccharification yield under the conditions used.					
32429136	6	62	theme	content	1167:1173	arg1	roles					1107:1111	the roles	1103:1111	the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment	1103:1202	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
32429136	6	63	theme	liquid	1184:1189	arg1	pretreatment					1191:1202	ionic liquid pretreatment	1178:1202	ionic liquid pretreatment	1178:1202	These findings help distinguish the roles of anion hydrogen bonding, solvent acidity, and water content on ionic liquid pretreatment and can aid with anion and water content selections for different applications.					
33080189	1	0	theme	fantastic	179:187	arg1	specializations					189:203	fantastic specializations	179:203	fantastic specializations	179:203	The appendicularian tunicate Oikopleura epitomizes the degree to which evolution can constrain both genome and cellular composition, while at the same time unleashing fantastic specializations.					
33080189	1	1	theme	cellular	123:130	arg1	composition					132:142	cellular composition	123:142	cellular composition	123:142	The appendicularian tunicate Oikopleura epitomizes the degree to which evolution can constrain both genome and cellular composition, while at the same time unleashing fantastic specializations.					
33080189	1	2	theme	same	158:161	arg1	time					163:166	the same time	154:166	the same time	154:166	The appendicularian tunicate Oikopleura epitomizes the degree to which evolution can constrain both genome and cellular composition, while at the same time unleashing fantastic specializations.					
33080189	1	3	theme	tunicate	32:39	arg1	Oikopleura					41:50	The appendicularian tunicate Oikopleura	12:50	The appendicularian tunicate Oikopleura	12:50	The appendicularian tunicate Oikopleura epitomizes the degree to which evolution can constrain both genome and cellular composition, while at the same time unleashing fantastic specializations.					
33080189	1	4	theme	appendicularian	16:30	arg1	Oikopleura					41:50	The appendicularian tunicate Oikopleura	12:50	The appendicularian tunicate Oikopleura	12:50	The appendicularian tunicate Oikopleura epitomizes the degree to which evolution can constrain both genome and cellular composition, while at the same time unleashing fantastic specializations.					
33278949	0	0	theme	anticoagulant	73:85	arg1	properties					87:96	anticoagulant properties	73:96	anticoagulant properties	73:96	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	1	1	dep	Ulva	318:321	arg1	lactuca					323:329	lactuca	323:329	lactuca	323:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	8	2	dep	APTT	1093:1096	arg1	time					1114:1117	clotting time	1105:1117	clotting time	1105:1117	At high concentrations, acid extracts showed the highest APTT and TT clotting time.					
33278949	6	3	theme	normal	920:925	arg1	lymphocytes					944:954	normal peripheral blood lymphocytes	920:954	normal peripheral blood lymphocytes cells (PBL)	920:966	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	6	3	theme	normal	920:925	arg1	PBL					963:965	PBL	963:965	PBL	963:965	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	4	4	theme	Chemical	542:549	arg1	analyses					569:576	Chemical and spectroscopic analyses	542:576	Chemical and spectroscopic analyses	542:576	Chemical and spectroscopic analyses demonstrated that ulvans were constituted of (1→4)-β-glucuronic acid, (1→3,4)-α-L-rhamnose-3-sulphate and (1→4)-α-xylose.					
33278949	8	5	theme	acid	1060:1063	arg1	extracts					1065:1072	acid extracts	1060:1072	acid extracts	1060:1072	At high concentrations, acid extracts showed the highest APTT and TT clotting time.					
33278949	6	6	dep	lymphocytes	944:954	arg1	cells					956:960	cells	956:960	normal peripheral blood lymphocytes cells (PBL)	920:966	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	1	7	from	seaweed	310:316	arg1	ulvan					288:292	the sulphated polysaccharide 'ulvan'	258:293	the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	258:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	1	7	from	seaweed	310:316	arg1	composition					191:201	chemical composition	182:201	chemical composition	182:201	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	1	7	from	seaweed	310:316	arg1	properties					244:253	structural, antitumor and anticoagulant properties	204:253	structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	204:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	2	8	theme	spectroscopic	457:469	arg1	1H					442:443	1H	442:443	1H	442:443	The structural features of ulvans were carried out by FTIR and by one- and two- dimensional 1H and 13C NMR spectroscopic.					
33278949	4	9	theme	1→3,4	649:653	arg1	-α-L-rhamnose-3-sulphate					655:678	(1→3,4)-α-L-rhamnose-3-sulphate	648:678	(1→3,4)-α-L-rhamnose-3-sulphate	648:678	Chemical and spectroscopic analyses demonstrated that ulvans were constituted of (1→4)-β-glucuronic acid, (1→3,4)-α-L-rhamnose-3-sulphate and (1→4)-α-xylose.					
33278949	4	9	theme	1→3,4	649:653	arg1	acid					642:645	-β-glucuronic acid	628:645	-β-glucuronic acid	628:645	Chemical and spectroscopic analyses demonstrated that ulvans were constituted of (1→4)-β-glucuronic acid, (1→3,4)-α-L-rhamnose-3-sulphate and (1→4)-α-xylose.					
33278949	6	10	theme	blood	938:942	arg1	lymphocytes					944:954	normal peripheral blood lymphocytes	920:954	normal peripheral blood lymphocytes cells (PBL)	920:966	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	6	10	theme	blood	938:942	arg1	PBL					963:965	PBL	963:965	PBL	963:965	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	0	11	from	Effect	0:5	arg1	antitumor					59:67	antitumor	59:67	antitumor	59:67	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	0	11	from	Effect	0:5	arg1	properties					87:96	anticoagulant properties	73:96	anticoagulant properties	73:96	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	0	11	from	Effect	0:5	arg1	structure					48:56	the chemical structure	35:56	the chemical structure	35:56	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	1	12	from	effect	147:152	arg1	composition					191:201	chemical composition	182:201	chemical composition	182:201	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	1	12	from	effect	147:152	arg1	properties					244:253	structural, antitumor and anticoagulant properties	204:253	structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	204:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	4	13	theme	spectroscopic	555:567	arg1	analyses					569:576	Chemical and spectroscopic analyses	542:576	Chemical and spectroscopic analyses	542:576	Chemical and spectroscopic analyses demonstrated that ulvans were constituted of (1→4)-β-glucuronic acid, (1→3,4)-α-L-rhamnose-3-sulphate and (1→4)-α-xylose.					
33278949	0	14	from	structure	48:56	arg1	lactuca					117:123	Ulva lactuca	112:123	Ulva lactuca of Tunisia coast	112:140	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	1	15	theme	sulphated	262:270	arg1	ulvan					288:292	the sulphated polysaccharide 'ulvan'	258:293	the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	258:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	9	16	from	activities	1150:1159	arg1	lactuca					1181:1187	Ulva lactuca	1176:1187	Ulva lactuca	1176:1187	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca promote their use as effective therapeutic agent.					
33278949	2	17	theme	ulvans	377:382	arg1	features					365:372	The structural features	350:372	The structural features of ulvans	350:382	The structural features of ulvans were carried out by FTIR and by one- and two- dimensional 1H and 13C NMR spectroscopic.					
33278949	0	18	theme	ulvan	101:105	arg1	antitumor					59:67	antitumor	59:67	antitumor	59:67	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	0	18	theme	ulvan	101:105	arg1	properties					87:96	anticoagulant properties	73:96	anticoagulant properties	73:96	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	0	18	theme	ulvan	101:105	arg1	structure					48:56	the chemical structure	35:56	the chemical structure	35:56	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	6	19	theme	cancer	861:866	arg1	cells					868:872	cervical cancer cells	852:872	cervical cancer cells (HeLa) (IC50 = 1000 μg/mL)	852:899	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	6	19	theme	cancer	861:866	arg1	HeLa					875:878	HeLa	875:878	HeLa	875:878	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	7	20	theme	PBL	1025:1027	arg1	cells					1029:1033	PBL cells	1025:1033	PBL cells	1025:1033	Acid extracts promoted to reduce HeLa cells and to grow PBL cells.					
33278949	9	21	from	lactuca	1181:1187	arg1	ulvans					1164:1169	ulvans	1164:1169	ulvans from Ulva lactuca	1164:1187	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca promote their use as effective therapeutic agent.					
33278949	9	21	from	lactuca	1181:1187	arg1	activities					1150:1159	Antitumoral and anticoagulant activities	1120:1159	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca	1120:1187	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca promote their use as effective therapeutic agent.					
33278949	9	22	theme	ulvans	1164:1169	arg1	activities					1150:1159	Antitumoral and anticoagulant activities	1120:1159	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca	1120:1187	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca promote their use as effective therapeutic agent.					
33278949	1	23	theme	extraction	157:166	arg1	procedures					168:177	extraction procedures	157:177	extraction procedures	157:177	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	6	24	theme	cervical	852:859	arg1	cells					868:872	cervical cancer cells	852:872	cervical cancer cells (HeLa) (IC50 = 1000 μg/mL)	852:899	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	6	24	theme	cervical	852:859	arg1	HeLa					875:878	HeLa	875:878	HeLa	875:878	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	5	25	located	observed	738:745	arg1	Mw					770:771	Mw	770:771	Mw	770:771	The extraction procedures effect were observed in chemical structure, Mw and biological activities.					
33278949	5	25	located	observed	738:745	arg2	procedures					715:724	The extraction procedures	700:724	The extraction procedures effect	700:731	The extraction procedures effect were observed in chemical structure, Mw and biological activities.					
33278949	5	25	located	observed	738:745	arg1	structure					759:767	chemical structure	750:767	chemical structure	750:767	The extraction procedures effect were observed in chemical structure, Mw and biological activities.					
33278949	5	25	located	observed	738:745	arg1	activities					788:797	biological activities	777:797	biological activities	777:797	The extraction procedures effect were observed in chemical structure, Mw and biological activities.					
33278949	3	26	theme	uronic	529:534	arg1	acid					536:539	uronic acid	529:539	uronic acid	529:539	The ulvans were mainly composed of rhamnose, xylose, and uronic acid.					
33278949	1	27	theme	procedures	168:177	arg1	effect					147:152	The effect	143:152	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	143:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	1	28	from	properties	244:253	arg1	seaweed					310:316	the green seaweed	300:316	the green seaweed Ulva lactuca	300:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	1	29	theme	polysaccharide	272:285	arg1	ulvan					288:292	the sulphated polysaccharide 'ulvan'	258:293	the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	258:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	9	30	theme	Antitumoral	1120:1130	arg1	activities					1150:1159	Antitumoral and anticoagulant activities	1120:1159	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca	1120:1187	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca promote their use as effective therapeutic agent.					
33278949	4	31	theme	-β-glucuronic	628:640	arg1	-α-L-rhamnose-3-sulphate					655:678	(1→3,4)-α-L-rhamnose-3-sulphate	648:678	(1→3,4)-α-L-rhamnose-3-sulphate	648:678	Chemical and spectroscopic analyses demonstrated that ulvans were constituted of (1→4)-β-glucuronic acid, (1→3,4)-α-L-rhamnose-3-sulphate and (1→4)-α-xylose.					
33278949	4	31	theme	-β-glucuronic	628:640	arg1	acid					642:645	-β-glucuronic acid	628:645	-β-glucuronic acid	628:645	Chemical and spectroscopic analyses demonstrated that ulvans were constituted of (1→4)-β-glucuronic acid, (1→3,4)-α-L-rhamnose-3-sulphate and (1→4)-α-xylose.					
33278949	0	32	theme	procedures	21:30	arg1	Effect					0:5	Effect	0:5	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.	0:141	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	8	33	theme	highest	1085:1091	arg1	APTT					1093:1096	APTT	1093:1096	APTT	1093:1096	At high concentrations, acid extracts showed the highest APTT and TT clotting time.					
33278949	7	34	theme	HeLa	1002:1005	arg1	cells					1007:1011	HeLa cells	1002:1011	HeLa cells	1002:1011	Acid extracts promoted to reduce HeLa cells and to grow PBL cells.					
33278949	9	35	theme	anticoagulant	1136:1148	arg1	activities					1150:1159	Antitumoral and anticoagulant activities	1120:1159	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca	1120:1187	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca promote their use as effective therapeutic agent.					
33278949	1	36	theme	chemical	182:189	arg1	composition					191:201	chemical composition	182:201	chemical composition	182:201	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	1	36	theme	chemical	182:189	arg1	properties					244:253	structural, antitumor and anticoagulant properties	204:253	structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	204:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	0	37	theme	extraction	10:19	arg1	procedures					21:30	extraction procedures	10:30	extraction procedures	10:30	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	1	38	theme	ulvan	288:292	arg1	composition					191:201	chemical composition	182:201	chemical composition	182:201	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	1	38	theme	ulvan	288:292	arg1	properties					244:253	structural, antitumor and anticoagulant properties	204:253	structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	204:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	0	39	theme	Tunisia	128:134	arg1	coast					136:140	Tunisia coast	128:140	Tunisia coast	128:140	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	6	40	theme	extract	841:847	arg1	activity					810:817	Cytotoxic activity	800:817	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL)	800:899	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	8	41	theme	clotting	1105:1112	arg1	time					1114:1117	clotting time	1105:1117	clotting time	1105:1117	At high concentrations, acid extracts showed the highest APTT and TT clotting time.					
33278949	5	42	dep	procedures	715:724	arg1	effect					726:731	effect	726:731	The extraction procedures effect	700:731	The extraction procedures effect were observed in chemical structure, Mw and biological activities.					
33278949	0	43	from	antitumor	59:67	arg1	lactuca					117:123	Ulva lactuca	112:123	Ulva lactuca of Tunisia coast	112:140	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	2	44	theme	one-	416:419	arg1	1H					442:443	1H	442:443	1H	442:443	The structural features of ulvans were carried out by FTIR and by one- and two- dimensional 1H and 13C NMR spectroscopic.					
33278949	1	45	theme	structural	204:213	arg1	composition					191:201	chemical composition	182:201	chemical composition	182:201	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	1	45	theme	structural	204:213	arg1	properties					244:253	structural, antitumor and anticoagulant properties	204:253	structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	204:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	5	46	theme	chemical	750:757	arg1	structure					759:767	chemical structure	750:767	chemical structure	750:767	The extraction procedures effect were observed in chemical structure, Mw and biological activities.					
33278949	6	47	dep	cells	868:872	arg1	IC50					882:885	IC50	882:885	IC50 = 1000 μg/mL	882:898	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	6	48	dep	=	887:887	arg1	μg/mL					894:898	1000 μg/mL	889:898	1000 μg/mL	889:898	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	8	49	theme	high	1039:1042	arg1	concentrations					1044:1057	high concentrations	1039:1057	high concentrations	1039:1057	At high concentrations, acid extracts showed the highest APTT and TT clotting time.					
33278949	0	50	theme	chemical	39:46	arg1	structure					48:56	the chemical structure	35:56	the chemical structure	35:56	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	1	51	theme	green	304:308	arg1	seaweed					310:316	the green seaweed	300:316	the green seaweed Ulva lactuca	300:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	9	52	theme	therapeutic	1220:1230	arg1	agent					1232:1236	effective therapeutic agent	1210:1236	effective therapeutic agent	1210:1236	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca promote their use as effective therapeutic agent.					
33278949	2	53	theme	dimensional	430:440	arg1	1H					442:443	1H	442:443	1H	442:443	The structural features of ulvans were carried out by FTIR and by one- and two- dimensional 1H and 13C NMR spectroscopic.					
33278949	6	54	theme	enzymatic-chemical	822:839	arg1	extract					841:847	enzymatic-chemical extract	822:847	enzymatic-chemical extract	822:847	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	6	55	theme	=	887:887	arg1	IC50					882:885	IC50	882:885	IC50 = 1000 μg/mL	882:898	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	6	56	from	activity	810:817	arg1	cells					868:872	cervical cancer cells	852:872	cervical cancer cells (HeLa) (IC50 = 1000 μg/mL)	852:899	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	6	56	from	activity	810:817	arg1	HeLa					875:878	HeLa	875:878	HeLa	875:878	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	6	57	theme	Cytotoxic	800:808	arg1	activity					810:817	Cytotoxic activity	800:817	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL)	800:899	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	4	58	dep	acid	642:645	arg1	1→4					624:626	1→4	624:626	1→4	624:626	Chemical and spectroscopic analyses demonstrated that ulvans were constituted of (1→4)-β-glucuronic acid, (1→3,4)-α-L-rhamnose-3-sulphate and (1→4)-α-xylose.					
33278949	4	59	dep	-α-xylose	689:697	arg1	1→4					685:687	1→4	685:687	1→4	685:687	Chemical and spectroscopic analyses demonstrated that ulvans were constituted of (1→4)-β-glucuronic acid, (1→3,4)-α-L-rhamnose-3-sulphate and (1→4)-α-xylose.					
33278949	7	60	theme	Acid	969:972	arg1	extracts					974:981	Acid extracts	969:981	Acid extracts	969:981	Acid extracts promoted to reduce HeLa cells and to grow PBL cells.					
33278949	1	61	theme	antitumor	216:224	arg1	composition					191:201	chemical composition	182:201	chemical composition	182:201	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	1	61	theme	antitumor	216:224	arg1	properties					244:253	structural, antitumor and anticoagulant properties	204:253	structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	204:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	5	62	theme	extraction	704:713	arg1	procedures					715:724	The extraction procedures	700:724	The extraction procedures effect	700:731	The extraction procedures effect were observed in chemical structure, Mw and biological activities.					
33278949	0	63	from	properties	87:96	arg1	lactuca					117:123	Ulva lactuca	112:123	Ulva lactuca of Tunisia coast	112:140	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	9	64	theme	effective	1210:1218	arg1	agent					1232:1236	effective therapeutic agent	1210:1236	effective therapeutic agent	1210:1236	Antitumoral and anticoagulant activities of ulvans from Ulva lactuca promote their use as effective therapeutic agent.					
33278949	0	65	from	lactuca	117:123	arg1	ulvan					101:105	ulvan	101:105	ulvan from Ulva lactuca of Tunisia coast	101:140	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	0	65	from	lactuca	117:123	arg1	antitumor					59:67	antitumor	59:67	antitumor	59:67	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	0	65	from	lactuca	117:123	arg1	properties					87:96	anticoagulant properties	73:96	anticoagulant properties	73:96	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	0	65	from	lactuca	117:123	arg1	structure					48:56	the chemical structure	35:56	the chemical structure	35:56	Effect of extraction procedures on the chemical structure, antitumor and anticoagulant properties of ulvan from Ulva lactuca of Tunisia coast.					
33278949	6	66	theme	peripheral	927:936	arg1	lymphocytes					944:954	normal peripheral blood lymphocytes	920:954	normal peripheral blood lymphocytes cells (PBL)	920:966	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	6	66	theme	peripheral	927:936	arg1	PBL					963:965	PBL	963:965	PBL	963:965	Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC50 = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL).					
33278949	1	67	theme	Ulva	318:321	arg1	seaweed					310:316	the green seaweed	300:316	the green seaweed Ulva lactuca	300:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	5	68	theme	biological	777:786	arg1	activities					788:797	biological activities	777:797	biological activities	777:797	The extraction procedures effect were observed in chemical structure, Mw and biological activities.					
33278949	2	69	theme	structural	354:363	arg1	features					365:372	The structural features	350:372	The structural features of ulvans	350:382	The structural features of ulvans were carried out by FTIR and by one- and two- dimensional 1H and 13C NMR spectroscopic.					
33278949	1	70	theme	anticoagulant	230:242	arg1	composition					191:201	chemical composition	182:201	chemical composition	182:201	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
33278949	1	70	theme	anticoagulant	230:242	arg1	properties					244:253	structural, antitumor and anticoagulant properties	204:253	structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca	204:329	The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated.					
35007000	7	0	theme	3D	1254:1255	arg1	sensor					1277:1282	the 3D printed deformation sensor	1250:1282	the 3D printed deformation sensor	1250:1282	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	2	1	theme	dry	386:388	arg1	CNF					390:392	dry CNF	386:392	dry CNF	386:392	In this work, we introduced a new solvent drying approach to acquire dry CNF from aqueous suspensions and subsequently pyrolyzed the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology.					
35007000	6	2	theme	printed	1045:1051	arg1	shielding					1088:1096	3D printed electromagnetic interference (EMI) shielding	1042:1096	3D printed electromagnetic interference (EMI) shielding	1042:1096	The composite is also suitable for applications such as 3D printed electromagnetic interference (EMI) shielding and deformation sensing.					
35007000	7	3	theme	printed	1257:1263	arg1	sensor					1277:1282	the 3D printed deformation sensor	1250:1282	the 3D printed deformation sensor	1250:1282	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	1	4	theme	aqueous	190:196	arg1	suspensions					198:208	aqueous suspensions	190:208	aqueous suspensions	190:208	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	1	5	theme	ensuing	295:301	arg1	applications					303:314	ensuing applications	295:314	ensuing applications	295:314	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	0	6	theme	Conductive	128:137	arg1	Composites					139:148	Polycaprolactone Conductive Composites	111:148	Polycaprolactone Conductive Composites	111:148	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.					
35007000	8	7	theme	fibrous	1488:1494	arg1	CCNF					1496:1499	fibrous CCNF	1488:1499	fibrous CCNF from sustainable resources	1488:1526	Herein, this study sheds light on the development of multifunctional conductive composites embedded with fibrous CCNF from sustainable resources.					
35007000	7	8	theme	shielding	1156:1164	arg1	composite					1166:1174	the 3D printed EMI shielding composite	1137:1174	the 3D printed EMI shielding composite	1137:1174	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	7	9	theme	mechanical	1359:1368	arg1	distortions					1370:1380	mechanical distortions	1359:1380	mechanical distortions	1359:1380	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	4	10	theme	due	774:776	arg1	aggregation					762:772	CB aggregation	759:772	CB aggregation due to formation of interconnected three-dimensional (3D) structures	759:841	After being further mixed with carbon black (CB), the CCNF helped to minimize CB aggregation due to formation of interconnected three-dimensional (3D) structures.					
35007000	0	11	theme	Polycaprolactone	111:126	arg1	Composites					139:148	Polycaprolactone Conductive Composites	111:148	Polycaprolactone Conductive Composites	111:148	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.					
35007000	7	12	theme	GHz	1241:1243	arg1	range					1227:1231	the frequency range	1213:1231	the frequency range of 4-26 GHz	1213:1243	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	8	13	theme	embedded	1474:1481	arg1	composites					1463:1472	multifunctional conductive composites	1436:1472	multifunctional conductive composites embedded with fibrous CCNF from sustainable resources	1436:1526	Herein, this study sheds light on the development of multifunctional conductive composites embedded with fibrous CCNF from sustainable resources.					
35007000	5	14	theme	CCNF/CB/PCL	848:858	arg1	composite					860:868	The CCNF/CB/PCL composite	844:868	The CCNF/CB/PCL composite	844:868	The CCNF/CB/PCL composite exhibited superior electrical conductivity ascribed to electrons transporting more efficiently among CB aggregates.					
35007000	2	15	theme	CNF	450:452	arg1	precursor					454:462	the CNF precursor	446:462	the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology	446:533	In this work, we introduced a new solvent drying approach to acquire dry CNF from aqueous suspensions and subsequently pyrolyzed the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology.					
35007000	5	16	theme	superior	880:887	arg1	conductivity					900:911	superior electrical conductivity	880:911	superior electrical conductivity ascribed to electrons transporting more efficiently among CB aggregates	880:983	The CCNF/CB/PCL composite exhibited superior electrical conductivity ascribed to electrons transporting more efficiently among CB aggregates.					
35007000	2	17	theme	morphology	524:533	arg1	loss					504:507	loss	504:507	loss of its fibrous morphology	504:533	In this work, we introduced a new solvent drying approach to acquire dry CNF from aqueous suspensions and subsequently pyrolyzed the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology.					
35007000	7	18	theme	EMI	1152:1154	arg1	composite					1166:1174	the 3D printed EMI shielding composite	1137:1174	the 3D printed EMI shielding composite	1137:1174	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	2	19	theme	carbonized	474:483	arg1	CCNF					490:493	CCNF	490:493	CCNF	490:493	In this work, we introduced a new solvent drying approach to acquire dry CNF from aqueous suspensions and subsequently pyrolyzed the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology.					
35007000	2	19	theme	carbonized	474:483	arg1	CNF					485:487	carbonized CNF	474:487	carbonized CNF (CCNF)	474:494	In this work, we introduced a new solvent drying approach to acquire dry CNF from aqueous suspensions and subsequently pyrolyzed the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology.					
35007000	4	20	dep	three-dimensional	809:825	arg1	3D					828:829	3D	828:829	3D	828:829	After being further mixed with carbon black (CB), the CCNF helped to minimize CB aggregation due to formation of interconnected three-dimensional (3D) structures.					
35007000	0	21	theme	Cellulose	11:19	arg1	Nanofibril					21:30	Carbonized Cellulose Nanofibril	0:30	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.	0:149	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.					
35007000	8	22	from	resources	1518:1526	arg1	CCNF					1496:1499	fibrous CCNF	1488:1499	fibrous CCNF from sustainable resources	1488:1526	Herein, this study sheds light on the development of multifunctional conductive composites embedded with fibrous CCNF from sustainable resources.					
35007000	2	23	theme	fibrous	516:522	arg1	morphology					524:533	its fibrous morphology	512:533	its fibrous morphology	512:533	In this work, we introduced a new solvent drying approach to acquire dry CNF from aqueous suspensions and subsequently pyrolyzed the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology.					
35007000	2	24	theme	new	347:349	arg1	approach					366:373	a new solvent drying approach	345:373	a new solvent drying approach	345:373	In this work, we introduced a new solvent drying approach to acquire dry CNF from aqueous suspensions and subsequently pyrolyzed the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology.					
35007000	0	25	theme	Carbonized	0:9	arg1	Nanofibril					21:30	Carbonized Cellulose Nanofibril	0:30	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.	0:149	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.					
35007000	7	26	theme	3D	1141:1142	arg1	composite					1166:1174	the 3D printed EMI shielding composite	1137:1174	the 3D printed EMI shielding composite	1137:1174	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	7	27	theme	printed	1144:1150	arg1	composite					1166:1174	the 3D printed EMI shielding composite	1137:1174	the 3D printed EMI shielding composite	1137:1174	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	2	28	theme	aqueous	399:405	arg1	suspensions					407:417	aqueous suspensions	399:417	aqueous suspensions	399:417	In this work, we introduced a new solvent drying approach to acquire dry CNF from aqueous suspensions and subsequently pyrolyzed the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology.					
35007000	7	29	theme	frequency	1217:1225	arg1	range					1227:1231	the frequency range	1213:1231	the frequency range of 4-26 GHz	1213:1243	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	8	30	with	embedded	1474:1481	arg1	CCNF					1496:1499	fibrous CCNF	1488:1499	fibrous CCNF from sustainable resources	1488:1526	Herein, this study sheds light on the development of multifunctional conductive composites embedded with fibrous CCNF from sustainable resources.					
35007000	4	31	theme	structures	832:841	arg1	formation					781:789	formation	781:789	formation of interconnected three-dimensional (3D) structures	781:841	After being further mixed with carbon black (CB), the CCNF helped to minimize CB aggregation due to formation of interconnected three-dimensional (3D) structures.					
35007000	4	32	theme	CB	759:760	arg1	aggregation					762:772	CB aggregation	759:772	CB aggregation due to formation of interconnected three-dimensional (3D) structures	759:841	After being further mixed with carbon black (CB), the CCNF helped to minimize CB aggregation due to formation of interconnected three-dimensional (3D) structures.					
35007000	1	33	theme	aggregated	225:234	arg1	morphology					243:252	aggregated fibril morphology	225:252	aggregated fibril morphology	225:252	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	7	34	theme	excellent	1294:1302	arg1	sensitivity					1304:1314	excellent sensitivity	1294:1314	excellent sensitivity	1294:1314	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	1	35	theme	fibril	236:241	arg1	morphology					243:252	aggregated fibril morphology	225:252	aggregated fibril morphology	225:252	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	0	36	theme	Fibrous	52:58	arg1	Morphology					60:69	Individualized Fibrous Morphology	37:69	Individualized Fibrous Morphology	37:69	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.					
35007000	0	37	from	Applications	95:106	arg1	Composites					139:148	Polycaprolactone Conductive Composites	111:148	Polycaprolactone Conductive Composites	111:148	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.					
35007000	8	38	theme	composites	1463:1472	arg1	development					1421:1431	the development	1417:1431	the development of multifunctional conductive composites embedded with fibrous CCNF from sustainable resources	1417:1526	Herein, this study sheds light on the development of multifunctional conductive composites embedded with fibrous CCNF from sustainable resources.					
35007000	0	39	theme	Individualized	37:50	arg1	Morphology					60:69	Individualized Fibrous Morphology	37:69	Individualized Fibrous Morphology	37:69	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.					
35007000	6	40	theme	3D	1042:1043	arg1	shielding					1088:1096	3D printed electromagnetic interference (EMI) shielding	1042:1096	3D printed electromagnetic interference (EMI) shielding	1042:1096	The composite is also suitable for applications such as 3D printed electromagnetic interference (EMI) shielding and deformation sensing.					
35007000	3	41	theme	tensile	660:666	arg1	performance					668:678	PCL composite tensile performance	646:678	PCL composite tensile performance	646:678	The fibrous CCNF was dispersed homogeneously in polycaprolactone (PCL) thermoplastic resin, greatly enhancing PCL composite tensile performance.					
35007000	8	42	theme	conductive	1452:1461	arg1	composites					1463:1472	multifunctional conductive composites	1436:1472	multifunctional conductive composites embedded with fibrous CCNF from sustainable resources	1436:1526	Herein, this study sheds light on the development of multifunctional conductive composites embedded with fibrous CCNF from sustainable resources.					
35007000	7	43	theme	EM	1197:1198	arg1	radiation					1200:1208	EM radiation	1197:1208	EM radiation	1197:1208	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	7	44	theme	deformation	1265:1275	arg1	sensor					1277:1282	the 3D printed deformation sensor	1250:1282	the 3D printed deformation sensor	1250:1282	Specifically, the 3D printed EMI shielding composite efficiently absorbed EM radiation in the frequency range of 4-26 GHz, and the 3D printed deformation sensor exhibited excellent sensitivity, durability, and flexibility in monitoring mechanical distortions.					
35007000	0	45	with	Nanofibril	21:30	arg1	Morphology					60:69	Individualized Fibrous Morphology	37:69	Individualized Fibrous Morphology	37:69	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.					
35007000	1	46	theme	Drying	151:156	arg1	nanofibril					168:177	Drying cellulose nanofibril	151:177	Drying cellulose nanofibril (CNF) from aqueous suspensions	151:208	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	1	46	theme	Drying	151:156	arg1	CNF					180:182	CNF	180:182	CNF	180:182	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	1	47	from	performance	280:290	arg1	applications					303:314	ensuing applications	295:314	ensuing applications	295:314	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	3	48	theme	thermoplastic	607:619	arg1	resin					621:625	polycaprolactone (PCL) thermoplastic resin	584:625	polycaprolactone (PCL) thermoplastic resin	584:625	The fibrous CCNF was dispersed homogeneously in polycaprolactone (PCL) thermoplastic resin, greatly enhancing PCL composite tensile performance.					
35007000	1	49	theme	cellulose	158:166	arg1	nanofibril					168:177	Drying cellulose nanofibril	151:177	Drying cellulose nanofibril (CNF) from aqueous suspensions	151:208	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	1	49	theme	cellulose	158:166	arg1	CNF					180:182	CNF	180:182	CNF	180:182	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	4	50	theme	interconnected	794:807	arg1	structures					832:841	interconnected three-dimensional (3D) structures	794:841	interconnected three-dimensional (3D) structures	794:841	After being further mixed with carbon black (CB), the CCNF helped to minimize CB aggregation due to formation of interconnected three-dimensional (3D) structures.					
35007000	3	51	theme	PCL	646:648	arg1	performance					668:678	PCL composite tensile performance	646:678	PCL composite tensile performance	646:678	The fibrous CCNF was dispersed homogeneously in polycaprolactone (PCL) thermoplastic resin, greatly enhancing PCL composite tensile performance.					
35007000	2	52	theme	drying	359:364	arg1	approach					366:373	a new solvent drying approach	345:373	a new solvent drying approach	345:373	In this work, we introduced a new solvent drying approach to acquire dry CNF from aqueous suspensions and subsequently pyrolyzed the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology.					
35007000	8	53	theme	multifunctional	1436:1450	arg1	composites					1463:1472	multifunctional conductive composites	1436:1472	multifunctional conductive composites embedded with fibrous CCNF from sustainable resources	1436:1526	Herein, this study sheds light on the development of multifunctional conductive composites embedded with fibrous CCNF from sustainable resources.					
35007000	6	54	theme	EMI	1083:1085	arg1	shielding					1088:1096	3D printed electromagnetic interference (EMI) shielding	1042:1096	3D printed electromagnetic interference (EMI) shielding	1042:1096	The composite is also suitable for applications such as 3D printed electromagnetic interference (EMI) shielding and deformation sensing.					
35007000	3	55	theme	composite	650:658	arg1	performance					668:678	PCL composite tensile performance	646:678	PCL composite tensile performance	646:678	The fibrous CCNF was dispersed homogeneously in polycaprolactone (PCL) thermoplastic resin, greatly enhancing PCL composite tensile performance.					
35007000	2	56	theme	solvent	351:357	arg1	approach					366:373	a new solvent drying approach	345:373	a new solvent drying approach	345:373	In this work, we introduced a new solvent drying approach to acquire dry CNF from aqueous suspensions and subsequently pyrolyzed the CNF precursor to obtain carbonized CNF (CCNF) without loss of its fibrous morphology.					
35007000	5	57	theme	CB	971:972	arg1	aggregates					974:983	CB aggregates	971:983	CB aggregates	971:983	The CCNF/CB/PCL composite exhibited superior electrical conductivity ascribed to electrons transporting more efficiently among CB aggregates.					
35007000	0	58	theme	Multifunctional	79:93	arg1	Applications					95:106	Multifunctional Applications	79:106	Multifunctional Applications in Polycaprolactone Conductive Composites	79:148	Carbonized Cellulose Nanofibril with Individualized Fibrous Morphology: toward Multifunctional Applications in Polycaprolactone Conductive Composites.					
35007000	8	59	theme	sustainable	1506:1516	arg1	resources					1518:1526	sustainable resources	1506:1526	sustainable resources	1506:1526	Herein, this study sheds light on the development of multifunctional conductive composites embedded with fibrous CCNF from sustainable resources.					
35007000	6	60	theme	deformation	1102:1112	arg1	sensing					1114:1120	deformation sensing	1102:1120	deformation sensing	1102:1120	The composite is also suitable for applications such as 3D printed electromagnetic interference (EMI) shielding and deformation sensing.					
35007000	4	61	theme	black	719:723	arg1	carbon					712:717	carbon black	712:723	carbon black (CB)	712:728	After being further mixed with carbon black (CB), the CCNF helped to minimize CB aggregation due to formation of interconnected three-dimensional (3D) structures.					
35007000	4	61	theme	black	719:723	arg1	CB					726:727	CB	726:727	CB	726:727	After being further mixed with carbon black (CB), the CCNF helped to minimize CB aggregation due to formation of interconnected three-dimensional (3D) structures.					
35007000	5	62	theme	electrical	889:898	arg1	conductivity					900:911	superior electrical conductivity	880:911	superior electrical conductivity ascribed to electrons transporting more efficiently among CB aggregates	880:983	The CCNF/CB/PCL composite exhibited superior electrical conductivity ascribed to electrons transporting more efficiently among CB aggregates.					
35007000	6	63	theme	interference	1069:1080	arg1	shielding					1088:1096	3D printed electromagnetic interference (EMI) shielding	1042:1096	3D printed electromagnetic interference (EMI) shielding	1042:1096	The composite is also suitable for applications such as 3D printed electromagnetic interference (EMI) shielding and deformation sensing.					
35007000	4	64	theme	three-dimensional	809:825	arg1	structures					832:841	interconnected three-dimensional (3D) structures	794:841	interconnected three-dimensional (3D) structures	794:841	After being further mixed with carbon black (CB), the CCNF helped to minimize CB aggregation due to formation of interconnected three-dimensional (3D) structures.					
35007000	1	65	from	suspensions	198:208	arg1	nanofibril					168:177	Drying cellulose nanofibril	151:177	Drying cellulose nanofibril (CNF) from aqueous suspensions	151:208	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	1	65	from	suspensions	198:208	arg1	CNF					180:182	CNF	180:182	CNF	180:182	Drying cellulose nanofibril (CNF) from aqueous suspensions often leads to aggregated fibril morphology, negatively affecting its performance in ensuing applications.					
35007000	3	66	theme	fibrous	540:546	arg1	CCNF					548:551	The fibrous CCNF	536:551	The fibrous CCNF	536:551	The fibrous CCNF was dispersed homogeneously in polycaprolactone (PCL) thermoplastic resin, greatly enhancing PCL composite tensile performance.					
35007000	6	67	theme	electromagnetic	1053:1067	arg1	shielding					1088:1096	3D printed electromagnetic interference (EMI) shielding	1042:1096	3D printed electromagnetic interference (EMI) shielding	1042:1096	The composite is also suitable for applications such as 3D printed electromagnetic interference (EMI) shielding and deformation sensing.					
32780763	9	0	theme	Asteroleplasma	1594:1607	arg1	abundance					1609:1617	Asteroleplasma abundance	1594:1617	Asteroleplasma abundance	1594:1617	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	6	1	theme	Xuefeng	1151:1157	arg1	chickens					1170:1177	32 Xuefeng black-bone chickens	1148:1177	32 Xuefeng black-bone chickens	1148:1177	Next, we studied the intestinal microbial composition of 32 Xuefeng black-bone chickens.					
32780763	10	2	dep	showed	1760:1765	arg1	decreased					1842:1850	decreased	1842:1850	decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance	1842:1912	At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance.					
32780763	10	2	dep	showed	1760:1765	arg1	increased					1781:1789	increased	1781:1789	increased Bacteroides_vulgatus and Eubacterium_tortuosum	1781:1836	At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance.					
32780763	1	3	contain	contain	158:164	arg2	peptides					213:220	small peptides	207:220	small peptides	207:220	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	1	3	contain	contain	158:164	arg2	acids					183:187	abundant organic acids	166:187	abundant organic acids	166:187	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	1	3	contain	contain	158:164	arg1	Fermented					142:150	Fermented	142:150	Fermented	142:150	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	1	3	contain	contain	158:164	arg2	acids					196:200	amino acids	190:200	amino acids	190:200	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	1	3	contain	contain	158:164	arg2	morphology					275:284	morphology	275:284	morphology	275:284	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	4	4	theme	crypt	866:870	arg1	depth					872:876	crypt depth	866:876	crypt depth	866:876	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	6	5	theme	microbial	1123:1131	arg1	composition					1133:1143	the intestinal microbial composition	1108:1143	the intestinal microbial composition of 32 Xuefeng black-bone chickens	1108:1177	Next, we studied the intestinal microbial composition of 32 Xuefeng black-bone chickens.					
32780763	10	6	theme	Asteroleplasma_anaerobium	1989:2013	arg1	abundance					2015:2023	Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance	1963:2023	Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance	1963:2023	At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance.					
32780763	11	7	theme	jejunum	2083:2089	arg1	histomorphology					2091:2105	the jejunum histomorphology	2079:2105	the jejunum histomorphology	2079:2105	Moreover, bacterial abundance was closely related to the jejunum histomorphology.					
32780763	9	8	theme	crypt	1648:1652	arg1	depth					1654:1658	crypt depth	1648:1658	crypt depth	1648:1658	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	0	9	dep	morphology	73:82	arg1	the					58:60	the	58:60	the	58:60	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	10	10	theme	Mycoplasma_gallinarum	1963:1983	arg1	abundance					2015:2023	Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance	1963:2023	Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance	1963:2023	At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance.					
32780763	0	11	theme	black-bone	122:131	arg1	chicken					133:139	Xuefeng black-bone chicken	114:139	Xuefeng black-bone chicken	114:139	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	4	12	dep	depth	839:843	arg1	ratio					845:849	ratio	845:849	crypt depth ratio	833:849	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	3	13	theme	ginseng	626:632	arg1	polysaccharides					634:648	ginseng polysaccharides	626:648	ginseng polysaccharides	626:648	Here, Xuefeng black-bone chickens were fed a basal diet fermented by Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, and Enterococcus faecium, with or without ginseng polysaccharides.					
32780763	13	14	theme	depth	2445:2449	arg1	ratio					2451:2455	crypt depth ratio	2439:2455	crypt depth ratio	2439:2455	Mycoplasma_gallinarum abundance was positively correlated to villus height, and Bacteroides_vulgatus and Eubacterium_tortuosum abundance was positively correlated with villus height to crypt depth ratio and negatively correlated with crypt depth.					
32780763	5	15	theme	complete	906:913	arg1	feed					915:918	the 100% complete feed	897:918	the 100% complete feed	897:918	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	9	16	theme	villus	1689:1694	arg1	height					1696:1701	villus height	1689:1701	villus height to crypt depth ratio	1689:1722	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	4	17	theme	polysaccharide	740:753	arg1	groups					760:765	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups	651:765	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups	651:765	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	5	18	theme	crypt	1027:1031	arg1	depth					1033:1037	crypt depth	1027:1037	crypt depth	1027:1037	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	13	19	theme	villus	2422:2427	arg1	height					2429:2434	villus height	2422:2434	villus height to crypt depth ratio	2422:2455	Mycoplasma_gallinarum abundance was positively correlated to villus height, and Bacteroides_vulgatus and Eubacterium_tortuosum abundance was positively correlated with villus height to crypt depth ratio and negatively correlated with crypt depth.					
32780763	7	20	theme	most	1299:1302	arg1	phyla					1313:1317	the most dominant phyla	1295:1317	the most dominant phyla	1295:1317	A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla.					
32780763	7	20	theme	most	1299:1302	arg1	Proteobacteria					1256:1269	Proteobacteria	1256:1269	Proteobacteria	1256:1269	A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla.					
32780763	7	20	theme	most	1299:1302	arg1	Bacteroidetes					1276:1288	Bacteroidetes	1276:1288	Bacteroidetes	1276:1288	A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla.					
32780763	7	21	theme	phyla	1194:1198	arg1	total					1182:1186	A total	1180:1186	A total of 10 phyla and 442 genera	1180:1213	A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla.					
32780763	1	22	theme	intestine	320:328	arg1	morphology					275:284	morphology	275:284	morphology	275:284	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	1	22	theme	intestine	320:328	arg1	composition					301:311	microbiota composition	290:311	microbiota composition	290:311	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	1	22	theme	intestine	320:328	arg1	acids					183:187	abundant organic acids	166:187	abundant organic acids	166:187	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	1	22	theme	intestine	320:328	arg1	acids					196:200	amino acids	190:200	amino acids	190:200	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	1	22	theme	intestine	320:328	arg1	peptides					213:220	small peptides	207:220	small peptides	207:220	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	13	23	theme	Eubacterium_tortuosum	2359:2379	arg1	abundance					2381:2389	Eubacterium_tortuosum abundance	2359:2389	Eubacterium_tortuosum abundance	2359:2389	Mycoplasma_gallinarum abundance was positively correlated to villus height, and Bacteroides_vulgatus and Eubacterium_tortuosum abundance was positively correlated with villus height to crypt depth ratio and negatively correlated with crypt depth.					
32780763	4	24	theme	fermented	713:721	arg1	%					658:658	100% microbially fermented feed (Fe) and 100%	655:699	%	658:658	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	1	25	theme	organic	175:181	arg1	acids					183:187	abundant organic acids	166:187	abundant organic acids	166:187	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	0	26	from	Effects	0:6	arg1	morphology					73:82	intestinal morphology	62:82	intestinal morphology	62:82	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	0	26	from	Effects	0:6	arg1	composition					99:109	microbiota composition	88:109	microbiota composition	88:109	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	14	27	used	used	2564:2567	arg2	alternatives					2582:2593	effective alternatives	2572:2593	effective alternatives to antibiotics for improving intestinal morphology and microbial composition	2572:2670	Therefore, fermented feeds with ginseng polysaccharides may be used as effective alternatives to antibiotics for improving intestinal morphology and microbial composition.					
32780763	14	27	used	used	2564:2567	arg2	fermented					2512:2520	fermented	2512:2520	fermented	2512:2520	Therefore, fermented feeds with ginseng polysaccharides may be used as effective alternatives to antibiotics for improving intestinal morphology and microbial composition.					
32780763	0	28	theme	microbiota	88:97	arg1	composition					99:109	microbiota composition	88:109	microbiota composition	88:109	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	5	29	theme	villus	1056:1061	arg1	height					1063:1068	villus height	1056:1068	villus height	1056:1068	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	4	30	theme	ginseng	732:738	arg1	FP					756:757	FP	756:757	FP	756:757	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	4	30	theme	ginseng	732:738	arg1	polysaccharide					740:753	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide	651:753	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups	651:765	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	4	31	theme	villus	798:803	arg1	height					805:810	villus height	798:810	villus height	798:810	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	2	32	theme	improving	411:419	arg1	development					432:442	improving intestinal development	411:442	improving intestinal development	411:442	Ginseng polysaccharides exhibit several biological activities and contribute to improving intestinal development.					
32780763	9	33	theme	villus	1512:1517	arg1	height					1519:1524	villus height	1512:1524	villus height to crypt depth	1512:1539	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	5	34	theme	polysaccharide	932:945	arg1	group					952:956	ginseng polysaccharide (Po) group	924:956	ginseng polysaccharide (Po) group	924:956	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	5	34	theme	polysaccharide	932:945	arg1	Po					948:949	Po	948:949	Po	948:949	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	6	35	theme	chickens	1170:1177	arg1	composition					1133:1143	the intestinal microbial composition	1108:1143	the intestinal microbial composition of 32 Xuefeng black-bone chickens	1108:1177	Next, we studied the intestinal microbial composition of 32 Xuefeng black-bone chickens.					
32780763	14	36	dep	fermented	2512:2520	arg1	feeds					2522:2526	feeds	2522:2526	feeds	2522:2526	Therefore, fermented feeds with ginseng polysaccharides may be used as effective alternatives to antibiotics for improving intestinal morphology and microbial composition.					
32780763	4	37	theme	crypt	833:837	arg1	depth					839:843	crypt depth	833:843	crypt depth ratio	833:849	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	0	38	theme	fermented	11:19	arg1	Effects					0:6	Effects	0:6	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.	0:140	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	10	39	dep	showed	1932:1937	arg1	increased					1953:1961	increased	1953:1961	showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance	1932:2023	At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance.					
32780763	8	40	theme	Sutterella	1340:1349	arg1	abundance					1370:1378	Sutterella and Asteroleplasma abundance	1340:1378	Sutterella and Asteroleplasma abundance	1340:1378	At the genus level, Sutterella and Asteroleplasma abundance increased and decreased, respectively, in the FP and Po groups.					
32780763	1	41	theme	microbiota	290:299	arg1	composition					301:311	microbiota composition	290:311	microbiota composition	290:311	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	8	42	theme	Asteroleplasma	1355:1368	arg1	abundance					1370:1378	Sutterella and Asteroleplasma abundance	1340:1378	Sutterella and Asteroleplasma abundance	1340:1378	At the genus level, Sutterella and Asteroleplasma abundance increased and decreased, respectively, in the FP and Po groups.					
32780763	4	43	dep	%	658:658	arg1	Fe					688:689	Fe	688:689	Fe	688:689	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	4	43	dep	%	658:658	arg1	feed					682:685	fermented feed	672:685	fermented feed (Fe)	672:690	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	6	44	theme	black-bone	1159:1168	arg1	chickens					1170:1177	32 Xuefeng black-bone chickens	1148:1177	32 Xuefeng black-bone chickens	1148:1177	Next, we studied the intestinal microbial composition of 32 Xuefeng black-bone chickens.					
32780763	4	45	dep	showed	767:772	arg1	decreased					856:864	decreased	856:864	decreased crypt depth in the jejunum	856:891	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	4	45	dep	showed	767:772	arg1	increased					788:796	increased	788:796	increased villus height and villus height to crypt depth ratio	788:849	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	12	46	theme	crypt	2235:2239	arg1	ratio					2247:2251	crypt depth ratio	2235:2251	crypt depth ratio	2235:2251	Asteroleplasma_anaerobium abundance was positively correlated with crypt depth and negatively correlated with villus height to crypt depth ratio.					
32780763	0	47	dep	fermented	11:19	arg1	feeds					21:25	feeds	21:25	feeds	21:25	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	0	47	dep	fermented	11:19	arg1	polysaccharides					39:53	polysaccharides	39:53	polysaccharides	39:53	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	5	48	theme	crypt	980:984	arg1	ratio					992:996	crypt depth ratio	980:996	crypt depth ratio	980:996	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	2	49	theme	several	363:369	arg1	activities					382:391	several biological activities	363:391	several biological activities	363:391	Ginseng polysaccharides exhibit several biological activities and contribute to improving intestinal development.					
32780763	1	50	theme	nutritional	241:251	arg1	status					253:258	the nutritional status	237:258	the nutritional status	237:258	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	14	51	theme	intestinal	2624:2633	arg1	morphology					2635:2644	intestinal morphology	2624:2644	intestinal morphology	2624:2644	Therefore, fermented feeds with ginseng polysaccharides may be used as effective alternatives to antibiotics for improving intestinal morphology and microbial composition.					
32780763	11	52	theme	bacterial	2036:2044	arg1	abundance					2046:2054	bacterial abundance	2036:2054	bacterial abundance	2036:2054	Moreover, bacterial abundance was closely related to the jejunum histomorphology.					
32780763	10	53	theme	Po	1923:1924	arg1	group					1926:1930	the Po group	1919:1930	the Po group	1919:1930	At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance.					
32780763	10	54	theme	species	1732:1738	arg1	level					1740:1744	the species level	1728:1744	the species level	1728:1744	At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance.					
32780763	1	55	theme	amino	190:194	arg1	acids					196:200	amino acids	190:200	amino acids	190:200	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	6	56	theme	intestinal	1112:1121	arg1	composition					1133:1143	the intestinal microbial composition	1108:1143	the intestinal microbial composition of 32 Xuefeng black-bone chickens	1108:1177	Next, we studied the intestinal microbial composition of 32 Xuefeng black-bone chickens.					
32780763	12	57	theme	villus	2218:2223	arg1	height					2225:2230	villus height	2218:2230	villus height to crypt depth ratio	2218:2251	Asteroleplasma_anaerobium abundance was positively correlated with crypt depth and negatively correlated with villus height to crypt depth ratio.					
32780763	0	58	theme	chicken	133:139	arg1	morphology					73:82	intestinal morphology	62:82	intestinal morphology	62:82	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	0	58	theme	chicken	133:139	arg1	composition					99:109	microbiota composition	88:109	microbiota composition	88:109	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	5	59	theme	100	901:903	arg1	%					904:904	%	904:904	%	904:904	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	12	60	theme	crypt	2175:2179	arg1	depth					2181:2185	crypt depth	2175:2185	crypt depth	2175:2185	Asteroleplasma_anaerobium abundance was positively correlated with crypt depth and negatively correlated with villus height to crypt depth ratio.					
32780763	10	61	theme	FP	1751:1752	arg1	group					1754:1758	the FP group	1747:1758	the FP group	1747:1758	At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance.					
32780763	1	62	theme	small	207:211	arg1	peptides					213:220	small peptides	207:220	small peptides	207:220	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	4	63	theme	fermented	672:680	arg1	Fe					688:689	Fe	688:689	Fe	688:689	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	4	63	theme	fermented	672:680	arg1	feed					682:685	fermented feed	672:685	fermented feed (Fe)	672:690	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	5	64	theme	%	904:904	arg1	feed					915:918	the 100% complete feed	897:918	the 100% complete feed	897:918	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	9	65	dep	depth	1712:1716	arg1	ratio					1718:1722	ratio	1718:1722	crypt depth ratio	1706:1722	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	8	66	theme	FP	1426:1427	arg1	groups					1436:1441	the FP and Po groups	1422:1441	the FP and Po groups	1422:1441	At the genus level, Sutterella and Asteroleplasma abundance increased and decreased, respectively, in the FP and Po groups.					
32780763	4	67	theme	feed	723:726	arg1	FP					756:757	FP	756:757	FP	756:757	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	4	67	theme	feed	723:726	arg1	polysaccharide					740:753	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide	651:753	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups	651:765	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	9	68	theme	Sutterella	1444:1453	arg1	abundance					1455:1463	Sutterella abundance	1444:1463	Sutterella abundance	1444:1463	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	8	69	theme	Po	1433:1434	arg1	groups					1436:1441	the FP and Po groups	1422:1441	the FP and Po groups	1422:1441	At the genus level, Sutterella and Asteroleplasma abundance increased and decreased, respectively, in the FP and Po groups.					
32780763	9	70	dep	height	1501:1506	arg1	ratio					1541:1545	ratio	1541:1545	ratio	1541:1545	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	0	71	theme	intestinal	62:71	arg1	morphology					73:82	intestinal morphology	62:82	intestinal morphology	62:82	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	9	72	theme	crypt	1706:1710	arg1	depth					1712:1716	crypt depth	1706:1716	crypt depth ratio	1706:1722	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	1	73	dep	morphology	275:284	arg1	the					271:273	the	271:273	the	271:273	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	7	74	theme	dominant	1304:1311	arg1	phyla					1313:1317	the most dominant phyla	1295:1317	the most dominant phyla	1295:1317	A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla.					
32780763	7	74	theme	dominant	1304:1311	arg1	Proteobacteria					1256:1269	Proteobacteria	1256:1269	Proteobacteria	1256:1269	A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla.					
32780763	7	74	theme	dominant	1304:1311	arg1	Bacteroidetes					1276:1288	Bacteroidetes	1276:1288	Bacteroidetes	1276:1288	A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla.					
32780763	1	75	theme	abundant	166:173	arg1	acids					183:187	abundant organic acids	166:187	abundant organic acids	166:187	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	12	76	theme	Asteroleplasma_anaerobium	2108:2132	arg1	abundance					2134:2142	Asteroleplasma_anaerobium abundance	2108:2142	Asteroleplasma_anaerobium abundance	2108:2142	Asteroleplasma_anaerobium abundance was positively correlated with crypt depth and negatively correlated with villus height to crypt depth ratio.					
32780763	13	77	theme	crypt	2488:2492	arg1	depth					2494:2498	crypt depth	2488:2498	crypt depth	2488:2498	Mycoplasma_gallinarum abundance was positively correlated to villus height, and Bacteroides_vulgatus and Eubacterium_tortuosum abundance was positively correlated with villus height to crypt depth ratio and negatively correlated with crypt depth.					
32780763	9	78	theme	villus	1494:1499	arg1	height					1501:1506	villus height	1494:1506	villus height	1494:1506	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	1	79	dep	Fermented	142:150	arg1	feeds					152:156	feeds	152:156	feeds	152:156	Fermented feeds contain abundant organic acids, amino acids, and small peptides, which improve the nutritional status as well as the morphology and microbiota composition of the intestine.					
32780763	0	80	theme	Xuefeng	114:120	arg1	chicken					133:139	Xuefeng black-bone chicken	114:139	Xuefeng black-bone chicken	114:139	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	5	81	theme	ginseng	924:930	arg1	group					952:956	ginseng polysaccharide (Po) group	924:956	ginseng polysaccharide (Po) group	924:956	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	5	81	theme	ginseng	924:930	arg1	Po					948:949	Po	948:949	Po	948:949	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	3	82	theme	Xuefeng	451:457	arg1	chickens					470:477	Xuefeng black-bone chickens	451:477	Xuefeng black-bone chickens	451:477	Here, Xuefeng black-bone chickens were fed a basal diet fermented by Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, and Enterococcus faecium, with or without ginseng polysaccharides.					
32780763	9	83	theme	crypt	1577:1581	arg1	depth					1583:1587	crypt depth	1577:1587	crypt depth	1577:1587	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	9	84	theme	crypt	1529:1533	arg1	depth					1535:1539	crypt depth	1529:1539	crypt depth	1529:1539	Sutterella abundance was positively correlated to villus height and villus height to crypt depth ratio, and negatively correlated to crypt depth, and Asteroleplasma abundance was positively correlated to crypt depth and negatively correlated to villus height to crypt depth ratio.					
32780763	13	85	theme	villus	2315:2320	arg1	height					2322:2327	villus height	2315:2327	villus height	2315:2327	Mycoplasma_gallinarum abundance was positively correlated to villus height, and Bacteroides_vulgatus and Eubacterium_tortuosum abundance was positively correlated with villus height to crypt depth ratio and negatively correlated with crypt depth.					
32780763	8	86	theme	genus	1327:1331	arg1	level					1333:1337	the genus level	1323:1337	the genus level	1323:1337	At the genus level, Sutterella and Asteroleplasma abundance increased and decreased, respectively, in the FP and Po groups.					
32780763	3	87	theme	basal	490:494	arg1	diet					496:499	a basal diet	488:499	a basal diet fermented by Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, and Enterococcus faecium	488:607	Here, Xuefeng black-bone chickens were fed a basal diet fermented by Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, and Enterococcus faecium, with or without ginseng polysaccharides.					
32780763	0	88	theme	ginseng	31:37	arg1	Effects					0:6	Effects	0:6	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.	0:140	Effects of fermented feeds and ginseng polysaccharides on the intestinal morphology and microbiota composition of Xuefeng black-bone chicken.					
32780763	13	89	theme	crypt	2439:2443	arg1	ratio					2451:2455	crypt depth ratio	2439:2455	crypt depth ratio	2439:2455	Mycoplasma_gallinarum abundance was positively correlated to villus height, and Bacteroides_vulgatus and Eubacterium_tortuosum abundance was positively correlated with villus height to crypt depth ratio and negatively correlated with crypt depth.					
32780763	5	90	theme	depth	986:990	arg1	ratio					992:996	crypt depth ratio	980:996	crypt depth ratio	980:996	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	3	91	theme	black-bone	459:468	arg1	chickens					470:477	Xuefeng black-bone chickens	451:477	Xuefeng black-bone chickens	451:477	Here, Xuefeng black-bone chickens were fed a basal diet fermented by Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, and Enterococcus faecium, with or without ginseng polysaccharides.					
32780763	14	92	with	fermented	2512:2520	arg1	polysaccharides					2541:2555	ginseng polysaccharides	2533:2555	ginseng polysaccharides	2533:2555	Therefore, fermented feeds with ginseng polysaccharides may be used as effective alternatives to antibiotics for improving intestinal morphology and microbial composition.					
32780763	14	93	theme	effective	2572:2580	arg1	alternatives					2582:2593	effective alternatives	2572:2593	effective alternatives to antibiotics for improving intestinal morphology and microbial composition	2572:2670	Therefore, fermented feeds with ginseng polysaccharides may be used as effective alternatives to antibiotics for improving intestinal morphology and microbial composition.					
32780763	14	93	theme	effective	2572:2580	arg1	fermented					2512:2520	fermented	2512:2520	fermented	2512:2520	Therefore, fermented feeds with ginseng polysaccharides may be used as effective alternatives to antibiotics for improving intestinal morphology and microbial composition.					
32780763	2	94	theme	intestinal	421:430	arg1	development					432:442	improving intestinal development	411:442	improving intestinal development	411:442	Ginseng polysaccharides exhibit several biological activities and contribute to improving intestinal development.					
32780763	10	95	theme	Asteroleplasma_anaerobium	1878:1902	arg1	abundance					1904:1912	Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance	1852:1912	abundance	1904:1912	At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance.					
32780763	2	96	theme	Ginseng	331:337	arg1	polysaccharides					339:353	Ginseng polysaccharides	331:353	Ginseng polysaccharides	331:353	Ginseng polysaccharides exhibit several biological activities and contribute to improving intestinal development.					
32780763	12	97	theme	depth	2241:2245	arg1	ratio					2247:2251	crypt depth ratio	2235:2251	crypt depth ratio	2235:2251	Asteroleplasma_anaerobium abundance was positively correlated with crypt depth and negatively correlated with villus height to crypt depth ratio.					
32780763	5	98	theme	villus	963:968	arg1	height					970:975	the villus height	959:975	the villus height to crypt depth ratio	959:996	In the 100% complete feed and ginseng polysaccharide (Po) group, the villus height to crypt depth ratio was significantly increased, crypt depth was reduced, and villus height remained unaffected.					
32780763	10	99	theme	Mycoplasma_gallinarum	1852:1872	arg1	abundance					1904:1912	Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance	1852:1912	abundance	1904:1912	At the species level, the FP group showed significantly increased Bacteroides_vulgatus and Eubacterium_tortuosum and decreased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance, and the Po group showed significantly increased Mycoplasma_gallinarum and Asteroleplasma_anaerobium abundance.					
32780763	13	100	theme	Mycoplasma_gallinarum	2254:2274	arg1	abundance					2276:2284	Mycoplasma_gallinarum abundance	2254:2284	Mycoplasma_gallinarum abundance	2254:2284	Mycoplasma_gallinarum abundance was positively correlated to villus height, and Bacteroides_vulgatus and Eubacterium_tortuosum abundance was positively correlated with villus height to crypt depth ratio and negatively correlated with crypt depth.					
32780763	14	101	theme	microbial	2650:2658	arg1	composition					2660:2670	microbial composition	2650:2670	microbial composition	2650:2670	Therefore, fermented feeds with ginseng polysaccharides may be used as effective alternatives to antibiotics for improving intestinal morphology and microbial composition.					
32780763	7	102	dep	Proteobacteria	1256:1269	arg1	Firmicutes					1244:1253	Firmicutes, Proteobacteria, and Bacteroidetes	1244:1288	Firmicutes	1244:1253	A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla.					
32780763	7	103	theme	genera	1208:1213	arg1	total					1182:1186	A total	1180:1186	A total of 10 phyla and 442 genera	1180:1213	A total of 10 phyla and 442 genera were identified, among which Firmicutes, Proteobacteria, and Bacteroidetes were the most dominant phyla.					
32780763	4	104	theme	villus	816:821	arg1	height					823:828	villus height	816:828	villus height	816:828	The 100% microbially fermented feed (Fe) and 100% microbially fermented feed and ginseng polysaccharide (FP) groups showed significantly increased villus height and villus height to crypt depth ratio, and decreased crypt depth in the jejunum.					
32780763	14	105	theme	ginseng	2533:2539	arg1	polysaccharides					2541:2555	ginseng polysaccharides	2533:2555	ginseng polysaccharides	2533:2555	Therefore, fermented feeds with ginseng polysaccharides may be used as effective alternatives to antibiotics for improving intestinal morphology and microbial composition.					
32780763	2	106	theme	biological	371:380	arg1	activities					382:391	several biological activities	363:391	several biological activities	363:391	Ginseng polysaccharides exhibit several biological activities and contribute to improving intestinal development.					
32691432	12	0	theme	cranberry	1862:1870	arg1	rich					1882:1885	rich	1882:1885	rich	1882:1885	Despite the fact that the cranberry pomace is rich in plant cell polysaccharides, it has been mainly explored as a source of phenolic antioxidants.					
32691432	12	0	theme	cranberry	1862:1870	arg1	pomace					1872:1877	the cranberry pomace	1858:1877	the cranberry pomace	1858:1877	Despite the fact that the cranberry pomace is rich in plant cell polysaccharides, it has been mainly explored as a source of phenolic antioxidants.					
32691432	7	1	theme	Longer	1190:1195	arg1	milling					1197:1203	Longer milling	1190:1203	Longer milling	1190:1203	Longer milling did not affect particle size but increased their roughness.					
32691432	2	2	theme	alkaline	415:422	arg1	method					424:429	microwave-assisted alkaline method	396:429	microwave-assisted alkaline method	396:429	Extraction of carbohydrates from pomace was investigated using microwave-assisted alkaline method and five commercial biocatalysts (pure endo-galactanase and four multienzyme biocatalysts).					
32691432	15	3	theme	oligosaccharides	2392:2407	arg1	characteristics					2363:2377	the characteristics	2359:2377	the characteristics of generated oligosaccharides	2359:2407	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	15	3	theme	oligosaccharides	2392:2407	arg1	study					2290:2294	The study	2286:2294	The study of the effects of these extraction techniques on the yield	2286:2353	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	13	4	theme	nondigestible	2053:2065	arg1	oligosaccharides					2067:2082	nondigestible oligosaccharides	2053:2082	nondigestible oligosaccharides	2053:2082	This study reveals the efficiency of cranberry pomace as a source of nondigestible oligosaccharides.					
32691432	13	5	theme	pomace	2031:2036	arg1	efficiency					2007:2016	the efficiency	2003:2016	the efficiency of cranberry pomace	2003:2036	This study reveals the efficiency of cranberry pomace as a source of nondigestible oligosaccharides.					
32691432	13	5	theme	pomace	2031:2036	arg1	source					2043:2048	a source	2041:2048	a source of nondigestible oligosaccharides	2041:2082	This study reveals the efficiency of cranberry pomace as a source of nondigestible oligosaccharides.					
32691432	16	6	theme	innovative	2568:2577	arg1	process					2579:2585	innovative process	2568:2585	innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products	2568:2697	As an overall, the findings of this study would contribute to lay the scientific ground for the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products.					
32691432	4	7	theme	5	904:904	arg1	polymerization					881:894	polymerization	881:894	polymerization of 2 to 5	881:904	All multienzyme biocatalysts led to yields similar or higher than microwave-assisted approach (23.4% to 42.0%), but mainly generated shorter oligosaccharides with a degree of polymerization of 2 to 5.					
32691432	5	8	theme	cranberry	919:927	arg1	fiber					944:948	cranberry pomace dietary fiber	919:948	cranberry pomace dietary fiber	919:948	Compared to cranberry pomace dietary fiber, microwave-assisted extracts were enriched in pectic oligosaccharides, whereas the enzymatic extracts were enriched in glucans and had less rhamnose and galactose.					
32691432	15	9	theme	generated	2382:2390	arg1	oligosaccharides					2392:2407	generated oligosaccharides	2382:2407	generated oligosaccharides	2382:2407	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	1	10	theme	juice	215:219	arg1	production					221:230	industrial juice production	204:230	industrial juice production	204:230	Cranberry pomace obtained from industrial juice production was characterized by proximate composition analysis and monosaccharide profile of the dietary fiber.					
32691432	8	11	theme	Such	1265:1268	arg1	changes					1279:1285	Such physical changes	1265:1285	Such physical changes	1265:1285	Such physical changes had no effect on the efficiency of multienzymatic treatment.					
32691432	8	12	theme	multienzymatic	1322:1335	arg1	treatment					1337:1345	multienzymatic treatment	1322:1345	multienzymatic treatment	1322:1345	Such physical changes had no effect on the efficiency of multienzymatic treatment.					
32691432	0	13	from	generation	86:95	arg1	macrocarpon					152:162	macrocarpon	152:162	macrocarpon	152:162	Comparison of enzymatic and microwave-assisted alkaline extraction approaches for the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace.					
32691432	0	13	from	generation	86:95	arg1	Pomace					165:170	Pomace	165:170	Pomace	165:170	Comparison of enzymatic and microwave-assisted alkaline extraction approaches for the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace.					
32691432	0	13	from	generation	86:95	arg1	Cranberry					131:139	Cranberry	131:139	Cranberry	131:139	Comparison of enzymatic and microwave-assisted alkaline extraction approaches for the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace.					
32691432	14	14	theme	molecular	2255:2263	arg1	distribution					2272:2283	molecular weight distribution	2255:2283	molecular weight distribution	2255:2283	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	7	15	theme	particle	1220:1227	arg1	size					1229:1232	particle size	1220:1232	particle size	1220:1232	Longer milling did not affect particle size but increased their roughness.					
32691432	9	16	theme	cranberry	1415:1423	arg1	products					1425:1432	cranberry products	1415:1432	cranberry products	1415:1432	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.					
32691432	4	17	theme	23.4	801:804	arg1	%					805:805	%	805:805	%	805:805	All multienzyme biocatalysts led to yields similar or higher than microwave-assisted approach (23.4% to 42.0%), but mainly generated shorter oligosaccharides with a degree of polymerization of 2 to 5.					
32691432	3	18	theme	polymerization	673:686	arg1	range					688:692	polymerization range	673:692	polymerization range of 7 to 10	673:703	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	11	19	theme	food	1818:1821	arg1	ingredients					1823:1833	functional food ingredients	1807:1833	functional food ingredients	1807:1833	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	11	19	theme	food	1818:1821	arg1	application					1792:1802	application	1792:1802	application	1792:1802	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	5	20	theme	pectic	996:1001	arg1	oligosaccharides					1003:1018	pectic oligosaccharides	996:1018	pectic oligosaccharides	996:1018	Compared to cranberry pomace dietary fiber, microwave-assisted extracts were enriched in pectic oligosaccharides, whereas the enzymatic extracts were enriched in glucans and had less rhamnose and galactose.					
32691432	16	21	theme	cranberry	2670:2678	arg1	pomace					2680:2685	cranberry pomace	2670:2685	cranberry pomace by products	2670:2697	As an overall, the findings of this study would contribute to lay the scientific ground for the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products.					
32691432	9	22	theme	PRACTICAL	1348:1356	arg1	APPLICATION					1358:1368	PRACTICAL APPLICATION	1348:1368	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.	1348:1548	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.					
32691432	14	23	theme	monosaccharide	2224:2237	arg1	composition					2239:2249	well-defined monosaccharide composition	2211:2249	well-defined monosaccharide composition	2211:2249	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	15	24	from	study	2290:2294	arg1	yield					2349:2353	the yield	2345:2353	the yield	2345:2353	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	12	25	theme	phenolic	1961:1968	arg1	antioxidants					1970:1981	phenolic antioxidants	1961:1981	phenolic antioxidants	1961:1981	Despite the fact that the cranberry pomace is rich in plant cell polysaccharides, it has been mainly explored as a source of phenolic antioxidants.					
32691432	2	26	from	pomace	366:371	arg1	Extraction					333:342	Extraction	333:342	Extraction of carbohydrates from pomace	333:371	Extraction of carbohydrates from pomace was investigated using microwave-assisted alkaline method and five commercial biocatalysts (pure endo-galactanase and four multienzyme biocatalysts).					
32691432	0	27	theme	oligosaccharides	100:115	arg1	generation					86:95	the generation	82:95	the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace	82:170	Comparison of enzymatic and microwave-assisted alkaline extraction approaches for the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace.					
32691432	9	28	theme	increased	1375:1383	arg1	production					1385:1394	The increased production	1371:1394	The increased production of cranberries and cranberry products	1371:1432	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.					
32691432	15	29	theme	extraction	2320:2329	arg1	techniques					2331:2340	these extraction techniques	2314:2340	these extraction techniques	2314:2340	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	10	30	theme	%	1558:1558	arg1	%					1565:1565	60% to 70%	1556:1565	60% to 70% of cranberry pomace	1556:1585	Up to 60% to 70% of cranberry pomace is composed of plant cell wall material.					
32691432	10	30	theme	%	1558:1558	arg1	pomace					1580:1585	cranberry pomace	1570:1585	cranberry pomace	1570:1585	Up to 60% to 70% of cranberry pomace is composed of plant cell wall material.					
32691432	4	31	theme	shorter	839:845	arg1	oligosaccharides					847:862	shorter oligosaccharides	839:862	shorter oligosaccharides	839:862	All multienzyme biocatalysts led to yields similar or higher than microwave-assisted approach (23.4% to 42.0%), but mainly generated shorter oligosaccharides with a degree of polymerization of 2 to 5.					
32691432	2	32	theme	pure	465:468	arg1	endo-galactanase					470:485	pure endo-galactanase	465:485	pure endo-galactanase	465:485	Extraction of carbohydrates from pomace was investigated using microwave-assisted alkaline method and five commercial biocatalysts (pure endo-galactanase and four multienzyme biocatalysts).					
32691432	10	33	dep	%	1565:1565	arg1	to					1560:1561	to	1560:1561	to	1560:1561	Up to 60% to 70% of cranberry pomace is composed of plant cell wall material.					
32691432	1	34	theme	dietary	318:324	arg1	fiber					326:330	the dietary fiber	314:330	the dietary fiber	314:330	Cranberry pomace obtained from industrial juice production was characterized by proximate composition analysis and monosaccharide profile of the dietary fiber.					
32691432	4	35	dep	%	805:805	arg1	to					807:808	to	807:808	to	807:808	All multienzyme biocatalysts led to yields similar or higher than microwave-assisted approach (23.4% to 42.0%), but mainly generated shorter oligosaccharides with a degree of polymerization of 2 to 5.					
32691432	2	36	theme	multienzyme	496:506	arg1	biocatalysts					508:519	four multienzyme biocatalysts	491:519	four multienzyme biocatalysts	491:519	Extraction of carbohydrates from pomace was investigated using microwave-assisted alkaline method and five commercial biocatalysts (pure endo-galactanase and four multienzyme biocatalysts).					
32691432	0	37	theme	enzymatic	14:22	arg1	extraction					56:65	enzymatic and microwave-assisted alkaline extraction	14:65	enzymatic and microwave-assisted alkaline extraction	14:65	Comparison of enzymatic and microwave-assisted alkaline extraction approaches for the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace.					
32691432	9	38	theme	products	1425:1432	arg1	production					1385:1394	The increased production	1371:1394	The increased production of cranberries and cranberry products	1371:1432	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.					
32691432	9	39	dep	APPLICATION	1358:1368	arg1	generate					1452:1459	generate	1452:1459	will continuously generate more pomace, a potentially valuable material for the generation of added-value products	1434:1547	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.					
32691432	14	40	theme	biocatalysts	2140:2151	arg1	use					2089:2091	The use	2085:2091	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction	2085:2180	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	4	41	theme	polymerization	881:894	arg1	degree					871:876	a degree	869:876	a degree of polymerization of 2 to 5	869:904	All multienzyme biocatalysts led to yields similar or higher than microwave-assisted approach (23.4% to 42.0%), but mainly generated shorter oligosaccharides with a degree of polymerization of 2 to 5.					
32691432	0	42	theme	microwave-assisted	28:45	arg1	extraction					56:65	enzymatic and microwave-assisted alkaline extraction	14:65	enzymatic and microwave-assisted alkaline extraction	14:65	Comparison of enzymatic and microwave-assisted alkaline extraction approaches for the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace.					
32691432	16	43	theme	oligosaccharides	2622:2637	arg1	isolation					2595:2603	the isolation	2591:2603	the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products	2591:2697	As an overall, the findings of this study would contribute to lay the scientific ground for the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products.					
32691432	2	44	theme	microwave-assisted	396:413	arg1	method					424:429	microwave-assisted alkaline method	396:429	microwave-assisted alkaline method	396:429	Extraction of carbohydrates from pomace was investigated using microwave-assisted alkaline method and five commercial biocatalysts (pure endo-galactanase and four multienzyme biocatalysts).					
32691432	11	45	theme	plant	1666:1670	arg1	polysaccharides					1682:1696	naturally occurring plant cell wall polysaccharides	1646:1696	naturally occurring plant cell wall polysaccharides	1646:1696	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	3	46	theme	average	582:588	arg1	yield					603:607	average total sugars yield	582:607	average total sugars yield of 21.3%	582:616	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	0	47	theme	extraction	56:65	arg1	Comparison					0:9	Comparison	0:9	Comparison of enzymatic and microwave-assisted alkaline extraction	0:65	Comparison of enzymatic and microwave-assisted alkaline extraction approaches for the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace.					
32691432	12	48	theme	cell	1896:1899	arg1	polysaccharides					1901:1915	plant cell polysaccharides	1890:1915	plant cell polysaccharides	1890:1915	Despite the fact that the cranberry pomace is rich in plant cell polysaccharides, it has been mainly explored as a source of phenolic antioxidants.					
32691432	16	49	theme	functional	2642:2651	arg1	ingredients					2653:2663	functional ingredients	2642:2663	functional ingredients from cranberry pomace by products	2642:2697	As an overall, the findings of this study would contribute to lay the scientific ground for the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products.					
32691432	12	50	theme	plant	1890:1894	arg1	polysaccharides					1901:1915	plant cell polysaccharides	1890:1915	plant cell polysaccharides	1890:1915	Despite the fact that the cranberry pomace is rich in plant cell polysaccharides, it has been mainly explored as a source of phenolic antioxidants.					
32691432	3	51	contain	contained	622:630	arg2	oligosaccharides					639:654	oligosaccharides	639:654	oligosaccharides in the degree of polymerization range of 7 to 10	639:703	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	3	51	contain	contained	622:630	arg1	extracts					527:534	The extracts	523:534	The extracts obtained from microwave-assisted approach	523:576	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	10	52	theme	pomace	1580:1585	arg1	%					1565:1565	60% to 70%	1556:1565	60% to 70% of cranberry pomace	1556:1585	Up to 60% to 70% of cranberry pomace is composed of plant cell wall material.					
32691432	10	52	theme	pomace	1580:1585	arg1	pomace					1580:1585	cranberry pomace	1570:1585	cranberry pomace	1570:1585	Up to 60% to 70% of cranberry pomace is composed of plant cell wall material.					
32691432	2	53	dep	biocatalysts	451:462	arg1	biocatalysts					508:519	four multienzyme biocatalysts	491:519	four multienzyme biocatalysts	491:519	Extraction of carbohydrates from pomace was investigated using microwave-assisted alkaline method and five commercial biocatalysts (pure endo-galactanase and four multienzyme biocatalysts).					
32691432	2	53	dep	biocatalysts	451:462	arg1	endo-galactanase					470:485	pure endo-galactanase	465:485	pure endo-galactanase	465:485	Extraction of carbohydrates from pomace was investigated using microwave-assisted alkaline method and five commercial biocatalysts (pure endo-galactanase and four multienzyme biocatalysts).					
32691432	1	54	theme	proximate	253:261	arg1	analysis					275:282	proximate composition analysis	253:282	proximate composition analysis	253:282	Cranberry pomace obtained from industrial juice production was characterized by proximate composition analysis and monosaccharide profile of the dietary fiber.					
32691432	13	55	theme	oligosaccharides	2067:2082	arg1	efficiency					2007:2016	the efficiency	2003:2016	the efficiency of cranberry pomace	2003:2036	This study reveals the efficiency of cranberry pomace as a source of nondigestible oligosaccharides.					
32691432	13	55	theme	oligosaccharides	2067:2082	arg1	source					2043:2048	a source	2041:2048	a source of nondigestible oligosaccharides	2041:2082	This study reveals the efficiency of cranberry pomace as a source of nondigestible oligosaccharides.					
32691432	6	56	theme	powdery	1169:1175	arg1	consistence					1177:1187	a powdery consistence	1167:1187	a powdery consistence	1167:1187	Pomace ground for 5 min or more by ball mill assumed a powdery consistence.					
32691432	10	57	theme	wall	1613:1616	arg1	material					1618:1625	plant cell wall material	1602:1625	plant cell wall material	1602:1625	Up to 60% to 70% of cranberry pomace is composed of plant cell wall material.					
32691432	11	58	theme	them	1782:1785	arg1	them					1782:1785	them	1782:1785	them	1782:1785	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	11	58	theme	them	1782:1785	arg1	many					1774:1777	many	1774:1777	many	1774:1777	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	9	59	theme	added-value	1528:1538	arg1	products					1540:1547	added-value products	1528:1547	added-value products	1528:1547	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.					
32691432	10	60	theme	plant	1602:1606	arg1	material					1618:1625	plant cell wall material	1602:1625	plant cell wall material	1602:1625	Up to 60% to 70% of cranberry pomace is composed of plant cell wall material.					
32691432	11	61	theme	wall	1677:1680	arg1	polysaccharides					1682:1696	naturally occurring plant cell wall polysaccharides	1646:1696	naturally occurring plant cell wall polysaccharides	1646:1696	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	3	62	theme	sugars	596:601	arg1	yield					603:607	average total sugars yield	582:607	average total sugars yield of 21.3%	582:616	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	1	63	theme	monosaccharide	288:301	arg1	profile					303:309	monosaccharide profile	288:309	monosaccharide profile	288:309	Cranberry pomace obtained from industrial juice production was characterized by proximate composition analysis and monosaccharide profile of the dietary fiber.					
32691432	15	64	from	yield	2349:2353	arg1	characteristics					2363:2377	the characteristics	2359:2377	the characteristics of generated oligosaccharides	2359:2407	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	15	64	from	yield	2349:2353	arg1	study					2290:2294	The study	2286:2294	The study of the effects of these extraction techniques on the yield	2286:2353	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	6	65	theme	ball	1149:1152	arg1	mill					1154:1157	ball mill	1149:1157	ball mill	1149:1157	Pomace ground for 5 min or more by ball mill assumed a powdery consistence.					
32691432	11	66	theme	corresponding	1708:1720	arg1	oligosaccharides					1722:1737	their corresponding oligosaccharides	1702:1737	their corresponding oligosaccharides	1702:1737	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	13	67	theme	cranberry	2021:2029	arg1	pomace					2031:2036	cranberry pomace	2021:2036	cranberry pomace	2021:2036	This study reveals the efficiency of cranberry pomace as a source of nondigestible oligosaccharides.					
32691432	16	68	theme	process	2579:2585	arg1	development					2553:2563	the development	2549:2563	the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products	2549:2697	As an overall, the findings of this study would contribute to lay the scientific ground for the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products.					
32691432	14	69	with	oligosaccharides	2189:2204	arg1	composition					2239:2249	well-defined monosaccharide composition	2211:2249	well-defined monosaccharide composition	2211:2249	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	14	69	with	oligosaccharides	2189:2204	arg1	distribution					2272:2283	molecular weight distribution	2255:2283	molecular weight distribution	2255:2283	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	5	70	theme	microwave-assisted	951:968	arg1	extracts					970:977	microwave-assisted extracts	951:977	microwave-assisted extracts	951:977	Compared to cranberry pomace dietary fiber, microwave-assisted extracts were enriched in pectic oligosaccharides, whereas the enzymatic extracts were enriched in glucans and had less rhamnose and galactose.					
32691432	11	71	theme	great	1754:1758	arg1	interest					1760:1767	a great interest	1752:1767	a great interest	1752:1767	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	3	72	theme	%	616:616	arg1	yield					603:607	average total sugars yield	582:607	average total sugars yield of 21.3%	582:616	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	1	73	theme	industrial	204:213	arg1	production					221:230	industrial juice production	204:230	industrial juice production	204:230	Cranberry pomace obtained from industrial juice production was characterized by proximate composition analysis and monosaccharide profile of the dietary fiber.					
32691432	16	74	theme	scientific	2527:2536	arg1	ground					2538:2543	the scientific ground	2523:2543	the scientific ground for the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products	2523:2697	As an overall, the findings of this study would contribute to lay the scientific ground for the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products.					
32691432	5	75	theme	pomace	929:934	arg1	fiber					944:948	cranberry pomace dietary fiber	919:948	cranberry pomace dietary fiber	919:948	Compared to cranberry pomace dietary fiber, microwave-assisted extracts were enriched in pectic oligosaccharides, whereas the enzymatic extracts were enriched in glucans and had less rhamnose and galactose.					
32691432	2	76	theme	carbohydrates	347:359	arg1	Extraction					333:342	Extraction	333:342	Extraction of carbohydrates from pomace	333:371	Extraction of carbohydrates from pomace was investigated using microwave-assisted alkaline method and five commercial biocatalysts (pure endo-galactanase and four multienzyme biocatalysts).					
32691432	14	77	theme	weight	2265:2270	arg1	distribution					2272:2283	molecular weight distribution	2255:2283	molecular weight distribution	2255:2283	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	16	78	from	pomace	2680:2685	arg1	ingredients					2653:2663	functional ingredients	2642:2663	functional ingredients from cranberry pomace by products	2642:2697	As an overall, the findings of this study would contribute to lay the scientific ground for the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products.					
32691432	3	79	theme	range	688:692	arg1	degree					663:668	the degree	659:668	the degree of polymerization range of 7 to 10	659:703	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	5	80	theme	dietary	936:942	arg1	fiber					944:948	cranberry pomace dietary fiber	919:948	cranberry pomace dietary fiber	919:948	Compared to cranberry pomace dietary fiber, microwave-assisted extracts were enriched in pectic oligosaccharides, whereas the enzymatic extracts were enriched in glucans and had less rhamnose and galactose.					
32691432	3	81	dep	10	702:703	arg1	to					699:700	to	699:700	to	699:700	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	8	82	theme	physical	1270:1277	arg1	changes					1279:1285	Such physical changes	1265:1285	Such physical changes	1265:1285	Such physical changes had no effect on the efficiency of multienzymatic treatment.					
32691432	8	83	theme	treatment	1337:1345	arg1	efficiency					1308:1317	the efficiency	1304:1317	the efficiency of multienzymatic treatment	1304:1345	Such physical changes had no effect on the efficiency of multienzymatic treatment.					
32691432	14	84	theme	microwave-assisted	2096:2113	arg1	extraction					2115:2124	microwave-assisted extraction	2096:2124	microwave-assisted extraction	2096:2124	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	15	85	theme	techniques	2331:2340	arg1	effects					2303:2309	the effects	2299:2309	the effects of these extraction techniques on the yield	2299:2353	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	12	86	from	polysaccharides	1901:1915	arg1	rich					1882:1885	rich	1882:1885	rich	1882:1885	Despite the fact that the cranberry pomace is rich in plant cell polysaccharides, it has been mainly explored as a source of phenolic antioxidants.					
32691432	12	86	from	polysaccharides	1901:1915	arg1	pomace					1872:1877	the cranberry pomace	1858:1877	the cranberry pomace	1858:1877	Despite the fact that the cranberry pomace is rich in plant cell polysaccharides, it has been mainly explored as a source of phenolic antioxidants.					
32691432	0	87	from	Cranberry	131:139	arg1	generation					86:95	the generation	82:95	the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace	82:170	Comparison of enzymatic and microwave-assisted alkaline extraction approaches for the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace.					
32691432	0	87	from	Cranberry	131:139	arg1	oligosaccharides					100:115	oligosaccharides	100:115	oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace	100:170	Comparison of enzymatic and microwave-assisted alkaline extraction approaches for the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace.					
32691432	3	88	from	oligosaccharides	639:654	arg1	degree					663:668	the degree	659:668	the degree of polymerization range of 7 to 10	659:703	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	3	89	theme	10	702:703	arg1	range					688:692	polymerization range	673:692	polymerization range of 7 to 10	673:703	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	9	90	theme	products	1540:1547	arg1	generation					1514:1523	the generation	1510:1523	the generation of added-value products	1510:1547	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.					
32691432	4	91	theme	multienzyme	710:720	arg1	biocatalysts					722:733	All multienzyme biocatalysts	706:733	All multienzyme biocatalysts	706:733	All multienzyme biocatalysts led to yields similar or higher than microwave-assisted approach (23.4% to 42.0%), but mainly generated shorter oligosaccharides with a degree of polymerization of 2 to 5.					
32691432	15	92	theme	effects	2303:2309	arg1	characteristics					2363:2377	the characteristics	2359:2377	the characteristics of generated oligosaccharides	2359:2407	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	15	92	theme	effects	2303:2309	arg1	study					2290:2294	The study	2286:2294	The study of the effects of these extraction techniques on the yield	2286:2353	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	15	93	theme	properties	2445:2454	arg1	modulation					2425:2434	the modulation	2421:2434	the modulation of their properties	2421:2454	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	11	94	theme	functional	1807:1816	arg1	ingredients					1823:1833	functional food ingredients	1807:1833	functional food ingredients	1807:1833	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	11	94	theme	functional	1807:1816	arg1	application					1792:1802	application	1792:1802	application	1792:1802	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	12	95	theme	antioxidants	1970:1981	arg1	source					1951:1956	a source	1949:1956	a source of phenolic antioxidants	1949:1981	Despite the fact that the cranberry pomace is rich in plant cell polysaccharides, it has been mainly explored as a source of phenolic antioxidants.					
32691432	12	95	theme	antioxidants	1970:1981	arg1	it					1918:1919	it	1918:1919	it	1918:1919	Despite the fact that the cranberry pomace is rich in plant cell polysaccharides, it has been mainly explored as a source of phenolic antioxidants.					
32691432	1	96	theme	Cranberry	173:181	arg1	pomace					183:188	Cranberry pomace	173:188	Cranberry pomace obtained from industrial juice production	173:230	Cranberry pomace obtained from industrial juice production was characterized by proximate composition analysis and monosaccharide profile of the dietary fiber.					
32691432	14	97	theme	well-defined	2211:2222	arg1	composition					2239:2249	well-defined monosaccharide composition	2211:2249	well-defined monosaccharide composition	2211:2249	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	4	98	theme	microwave-assisted	772:789	arg1	approach					791:798	microwave-assisted approach	772:798	microwave-assisted approach (23.4% to 42.0%)	772:815	All multienzyme biocatalysts led to yields similar or higher than microwave-assisted approach (23.4% to 42.0%), but mainly generated shorter oligosaccharides with a degree of polymerization of 2 to 5.					
32691432	4	98	theme	microwave-assisted	772:789	arg1	%					814:814	23.4% to 42.0%	801:814	23.4% to 42.0%	801:814	All multienzyme biocatalysts led to yields similar or higher than microwave-assisted approach (23.4% to 42.0%), but mainly generated shorter oligosaccharides with a degree of polymerization of 2 to 5.					
32691432	5	99	theme	enzymatic	1033:1041	arg1	extracts					1043:1050	the enzymatic extracts	1029:1050	the enzymatic extracts	1029:1050	Compared to cranberry pomace dietary fiber, microwave-assisted extracts were enriched in pectic oligosaccharides, whereas the enzymatic extracts were enriched in glucans and had less rhamnose and galactose.					
32691432	3	100	contain	had	578:580	arg2	yield					603:607	average total sugars yield	582:607	average total sugars yield of 21.3%	582:616	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	3	100	contain	had	578:580	arg1	extracts					527:534	The extracts	523:534	The extracts obtained from microwave-assisted approach	523:576	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	15	101	from	effects	2303:2309	arg1	yield					2349:2353	the yield	2345:2353	the yield	2345:2353	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	5	102	dep	enriched	984:991	arg1	whereas					1021:1027	whereas	1021:1027	whereas	1021:1027	Compared to cranberry pomace dietary fiber, microwave-assisted extracts were enriched in pectic oligosaccharides, whereas the enzymatic extracts were enriched in glucans and had less rhamnose and galactose.					
32691432	9	103	theme	cranberries	1399:1409	arg1	production					1385:1394	The increased production	1371:1394	The increased production of cranberries and cranberry products	1371:1432	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.					
32691432	14	104	theme	enzymatic	2161:2169	arg1	extraction					2171:2180	the enzymatic extraction	2157:2180	the enzymatic extraction	2157:2180	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	4	105	theme	42.0	810:813	arg1	%					805:805	%	805:805	%	805:805	All multienzyme biocatalysts led to yields similar or higher than microwave-assisted approach (23.4% to 42.0%), but mainly generated shorter oligosaccharides with a degree of polymerization of 2 to 5.					
32691432	2	106	theme	commercial	440:449	arg1	biocatalysts					451:462	five commercial biocatalysts	435:462	five commercial biocatalysts (pure endo-galactanase and four multienzyme biocatalysts)	435:520	Extraction of carbohydrates from pomace was investigated using microwave-assisted alkaline method and five commercial biocatalysts (pure endo-galactanase and four multienzyme biocatalysts).					
32691432	4	107	dep	5	904:904	arg1	to					901:902	to	901:902	to	901:902	All multienzyme biocatalysts led to yields similar or higher than microwave-assisted approach (23.4% to 42.0%), but mainly generated shorter oligosaccharides with a degree of polymerization of 2 to 5.					
32691432	1	108	theme	fiber	326:330	arg1	analysis					275:282	proximate composition analysis	253:282	proximate composition analysis	253:282	Cranberry pomace obtained from industrial juice production was characterized by proximate composition analysis and monosaccharide profile of the dietary fiber.					
32691432	1	108	theme	fiber	326:330	arg1	profile					303:309	monosaccharide profile	288:309	monosaccharide profile	288:309	Cranberry pomace obtained from industrial juice production was characterized by proximate composition analysis and monosaccharide profile of the dietary fiber.					
32691432	8	109	contain	had	1287:1289	arg1	changes					1279:1285	Such physical changes	1265:1285	Such physical changes	1265:1285	Such physical changes had no effect on the efficiency of multienzymatic treatment.					
32691432	8	109	contain	had	1287:1289	arg2	effect					1294:1299	no effect	1291:1299	no effect	1291:1299	Such physical changes had no effect on the efficiency of multienzymatic treatment.					
32691432	3	110	theme	microwave-assisted	550:567	arg1	approach					569:576	microwave-assisted approach	550:576	microwave-assisted approach	550:576	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	0	111	theme	alkaline	47:54	arg1	extraction					56:65	enzymatic and microwave-assisted alkaline extraction	14:65	enzymatic and microwave-assisted alkaline extraction	14:65	Comparison of enzymatic and microwave-assisted alkaline extraction approaches for the generation of oligosaccharides from American Cranberry (Vaccinium macrocarpon) Pomace.					
32691432	6	112	theme	Pomace	1114:1119	arg1	ground					1121:1126	Pomace ground	1114:1126	Pomace ground for 5 min or more by ball mill	1114:1157	Pomace ground for 5 min or more by ball mill assumed a powdery consistence.					
32691432	14	113	theme	different	2130:2138	arg1	biocatalysts					2140:2151	different biocatalysts	2130:2151	different biocatalysts	2130:2151	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	11	114	theme	occurring	1656:1664	arg1	polysaccharides					1682:1696	naturally occurring plant cell wall polysaccharides	1646:1696	naturally occurring plant cell wall polysaccharides	1646:1696	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	16	115	theme	nondigestible	2608:2620	arg1	oligosaccharides					2622:2637	nondigestible oligosaccharides	2608:2637	nondigestible oligosaccharides as functional ingredients from cranberry pomace by products	2608:2697	As an overall, the findings of this study would contribute to lay the scientific ground for the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products.					
32691432	16	116	theme	study	2493:2497	arg1	findings					2476:2483	the findings	2472:2483	the findings of this study	2472:2497	As an overall, the findings of this study would contribute to lay the scientific ground for the development of innovative process for the isolation of nondigestible oligosaccharides as functional ingredients from cranberry pomace by products.					
32691432	14	117	theme	extraction	2115:2124	arg1	use					2089:2091	The use	2085:2091	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction	2085:2180	The use of microwave-assisted extraction and different biocatalysts for the enzymatic extraction led to oligosaccharides with well-defined monosaccharide composition and molecular weight distribution.					
32691432	3	118	theme	total	590:594	arg1	yield					603:607	average total sugars yield	582:607	average total sugars yield of 21.3%	582:616	The extracts obtained from microwave-assisted approach had average total sugars yield of 21.3% and contained mostly oligosaccharides in the degree of polymerization range of 7 to 10.					
32691432	15	119	from	characteristics	2363:2377	arg1	yield					2349:2353	the yield	2345:2353	the yield	2345:2353	The study of the effects of these extraction techniques on the yield and the characteristics of generated oligosaccharides would allow the modulation of their properties.					
32691432	12	120	from	rich	1882:1885	arg1	polysaccharides					1901:1915	plant cell polysaccharides	1890:1915	plant cell polysaccharides	1890:1915	Despite the fact that the cranberry pomace is rich in plant cell polysaccharides, it has been mainly explored as a source of phenolic antioxidants.					
32691432	10	121	theme	cranberry	1570:1578	arg1	pomace					1580:1585	cranberry pomace	1570:1585	cranberry pomace	1570:1585	Up to 60% to 70% of cranberry pomace is composed of plant cell wall material.					
32691432	11	122	theme	polysaccharides	1682:1696	arg1	properties					1632:1641	The properties	1628:1641	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides	1628:1737	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	1	123	theme	composition	263:273	arg1	analysis					275:282	proximate composition analysis	253:282	proximate composition analysis	253:282	Cranberry pomace obtained from industrial juice production was characterized by proximate composition analysis and monosaccharide profile of the dietary fiber.					
32691432	10	124	theme	cell	1608:1611	arg1	material					1618:1625	plant cell wall material	1602:1625	plant cell wall material	1602:1625	Up to 60% to 70% of cranberry pomace is composed of plant cell wall material.					
32691432	11	125	theme	oligosaccharides	1722:1737	arg1	properties					1632:1641	The properties	1628:1641	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides	1628:1737	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	11	126	theme	cell	1672:1675	arg1	polysaccharides					1682:1696	naturally occurring plant cell wall polysaccharides	1646:1696	naturally occurring plant cell wall polysaccharides	1646:1696	The properties of naturally occurring plant cell wall polysaccharides and their corresponding oligosaccharides have been of a great interest, and many of them find application as functional food ingredients.					
32691432	9	127	theme	valuable	1488:1495	arg1	material					1497:1504	a potentially valuable material	1474:1504	a potentially valuable material for the generation of added-value products	1474:1547	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.					
32691432	9	127	theme	valuable	1488:1495	arg1	pomace					1466:1471	pomace	1466:1471	pomace	1466:1471	PRACTICAL APPLICATION: The increased production of cranberries and cranberry products will continuously generate more pomace, a potentially valuable material for the generation of added-value products.					
32617945	0	0	theme	Cell	89:92	arg1	Walls					94:98	Cell Walls	89:98	Cell Walls from Plants and Marine Algae	89:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	1	1	theme	cell	146:149	arg1	composites					169:178	diverse composites	161:178	diverse composites of complex polysaccharides	161:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	1	1	theme	cell	146:149	arg1	polysaccharides					191:205	complex polysaccharides	183:205	complex polysaccharides	183:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	1	1	theme	cell	146:149	arg1	walls					151:155	Plant and algal cell walls	130:155	Plant and algal cell walls	130:155	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	0	2	from	Antibodies	11:20	arg1	Plants					105:110	Plants	105:110	Plants	105:110	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	2	from	Antibodies	11:20	arg1	Algae					123:127	Marine Algae	116:127	Marine Algae	116:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	2	from	Antibodies	11:20	arg1	Walls					94:98	Cell Walls	89:98	Cell Walls from Plants and Marine Algae	89:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	3	3	theme	marine	505:510	arg1	materials					518:526	plant and marine algal materials	495:526	materials	518:526	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	3	4	used	used	430:433	arg2	methods					410:416	methods	410:416	methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials	410:526	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	0	5	from	Detection	57:65	arg1	Plants					105:110	Plants	105:110	Plants	105:110	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	5	from	Detection	57:65	arg1	Algae					123:127	Marine Algae	116:127	Marine Algae	116:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	5	from	Detection	57:65	arg1	Walls					94:98	Cell Walls	89:98	Cell Walls from Plants and Marine Algae	89:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	3	6	theme	algal	512:516	arg1	materials					518:526	plant and marine algal materials	495:526	materials	518:526	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	2	7	theme	cell	344:347	arg1	structures					354:363	cell wall structures	344:363	cell wall structures	344:363	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	3	8	from	information	574:584	arg1	masking					593:599	the masking	589:599	the masking of polysaccharides that may prevent detection	589:645	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	3	9	theme	wall	450:453	arg1	epitopes					482:489	epitopes	482:489	epitopes	482:489	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	3	9	theme	wall	450:453	arg1	structures					470:479	cell wall polysaccharide structures	445:479	cell wall polysaccharide structures (epitopes)	445:490	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	4	10	theme	cell	724:727	arg1	polysaccharides					734:748	cell wall polysaccharides	724:748	cell wall polysaccharides	724:748	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	2	11	theme	carbohydrate-binding	266:285	arg1	modules					287:293	carbohydrate-binding modules	266:293	carbohydrate-binding modules (CBMs)	266:300	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	2	11	theme	carbohydrate-binding	266:285	arg1	CBMs					296:299	CBMs	296:299	CBMs	296:299	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	1	12	theme	diverse	161:167	arg1	composites					169:178	diverse composites	161:178	diverse composites of complex polysaccharides	161:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	1	12	theme	diverse	161:167	arg1	polysaccharides					191:205	complex polysaccharides	183:205	complex polysaccharides	183:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	1	12	theme	diverse	161:167	arg1	walls					151:155	Plant and algal cell walls	130:155	Plant and algal cell walls	130:155	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	0	13	from	Modules	44:50	arg1	Plants					105:110	Plants	105:110	Plants	105:110	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	13	from	Modules	44:50	arg1	Algae					123:127	Marine Algae	116:127	Marine Algae	116:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	13	from	Modules	44:50	arg1	Walls					94:98	Cell Walls	89:98	Cell Walls from Plants and Marine Algae	89:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	2	14	theme	important	306:314	arg1	modules					287:293	carbohydrate-binding modules	266:293	carbohydrate-binding modules (CBMs)	266:300	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	2	14	theme	important	306:314	arg1	tools					316:320	important tools	306:320	important tools to detect and dissect cell wall structures in these materials	306:382	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	2	14	theme	important	306:314	arg1	probes					218:223	Molecular probes	208:223	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs)	208:300	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	2	14	theme	important	306:314	arg1	antibodies					244:253	monoclonal antibodies	233:253	monoclonal antibodies (MABs)	233:260	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	3	15	theme	plant	495:499	arg1	materials					518:526	plant and marine algal materials	495:526	materials	518:526	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	0	16	theme	Monoclonal	0:9	arg1	Antibodies					11:20	Monoclonal Antibodies	0:20	Monoclonal Antibodies	0:20	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	3	17	theme	methods	410:416	arg1	account					399:405	an account	396:405	an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials	396:526	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	0	18	theme	Carbohydrate-Binding	23:42	arg1	Modules					44:50	Carbohydrate-Binding Modules	23:50	Carbohydrate-Binding Modules	23:50	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	4	19	theme	cell	805:808	arg1	wall					810:813	cell wall immunocytochemistry	805:833	cell wall immunocytochemistry	805:833	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	4	20	theme	polysaccharides	734:748	arg1	methods					754:760	methods	754:760	methods	754:760	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	4	20	theme	polysaccharides	734:748	arg1	polysaccharides					734:748	cell wall polysaccharides	724:748	cell wall polysaccharides	724:748	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	4	20	theme	polysaccharides	734:748	arg1	sets					716:719	sets	716:719	sets of cell wall polysaccharides	716:748	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	1	21	theme	complex	183:189	arg1	polysaccharides					191:205	complex polysaccharides	183:205	complex polysaccharides	183:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	0	22	theme	Marine	116:121	arg1	Algae					123:127	Marine Algae	116:127	Marine Algae	116:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	4	23	theme	wall	729:732	arg1	polysaccharides					734:748	cell wall polysaccharides	724:748	cell wall polysaccharides	724:748	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	1	24	theme	polysaccharides	191:205	arg1	composites					169:178	diverse composites	161:178	diverse composites of complex polysaccharides	161:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	1	24	theme	polysaccharides	191:205	arg1	polysaccharides					191:205	complex polysaccharides	183:205	complex polysaccharides	183:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	1	24	theme	polysaccharides	191:205	arg1	walls					151:155	Plant and algal cell walls	130:155	Plant and algal cell walls	130:155	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	2	25	theme	monoclonal	233:242	arg1	MABs					256:259	MABs	256:259	MABs	256:259	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	2	25	theme	monoclonal	233:242	arg1	antibodies					244:253	monoclonal antibodies	233:253	monoclonal antibodies (MABs)	233:260	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	4	26	dep	them	773:776	arg1	aspect					795:800	an important aspect	782:800	an important aspect of cell wall immunocytochemistry	782:833	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	3	27	theme	polysaccharides	604:618	arg1	masking					593:599	the masking	589:599	the masking of polysaccharides that may prevent detection	589:645	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	4	28	theme	wall	810:813	arg1	aspect					795:800	an important aspect	782:800	an important aspect of cell wall immunocytochemistry	782:833	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	3	29	theme	polysaccharide	455:468	arg1	epitopes					482:489	epitopes	482:489	epitopes	482:489	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	3	29	theme	polysaccharide	455:468	arg1	structures					470:479	cell wall polysaccharide structures	445:479	cell wall polysaccharide structures (epitopes)	445:490	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	2	30	theme	Molecular	208:216	arg1	modules					287:293	carbohydrate-binding modules	266:293	carbohydrate-binding modules (CBMs)	266:300	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	2	30	theme	Molecular	208:216	arg1	probes					218:223	Molecular probes	208:223	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs)	208:300	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	2	30	theme	Molecular	208:216	arg1	tools					316:320	important tools	306:320	important tools to detect and dissect cell wall structures in these materials	306:382	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	2	30	theme	Molecular	208:216	arg1	antibodies					244:253	monoclonal antibodies	233:253	monoclonal antibodies (MABs)	233:260	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
32617945	1	31	theme	algal	140:144	arg1	composites					169:178	diverse composites	161:178	diverse composites of complex polysaccharides	161:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	1	31	theme	algal	140:144	arg1	polysaccharides					191:205	complex polysaccharides	183:205	complex polysaccharides	183:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	1	31	theme	algal	140:144	arg1	walls					151:155	Plant and algal cell walls	130:155	Plant and algal cell walls	130:155	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	4	32	theme	potential	685:693	arg1	interactions					695:706	potential interactions	685:706	potential interactions between sets of cell wall polysaccharides and methods	685:760	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	0	33	from	Plants	105:110	arg1	Walls					94:98	Cell Walls	89:98	Cell Walls from Plants and Marine Algae	89:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	33	from	Plants	105:110	arg1	Antibodies					11:20	Monoclonal Antibodies	0:20	Monoclonal Antibodies	0:20	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	33	from	Plants	105:110	arg1	Modules					44:50	Carbohydrate-Binding Modules	23:50	Carbohydrate-Binding Modules	23:50	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	33	from	Plants	105:110	arg1	Detection					57:65	Detection	57:65	Detection of Polysaccharides in Cell Walls from Plants and Marine Algae	57:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	4	34	theme	masking	654:660	arg1	phenomena					662:670	These masking phenomena	648:670	These masking phenomena	648:670	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	3	35	theme	cell	445:448	arg1	epitopes					482:489	epitopes	482:489	epitopes	482:489	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	3	35	theme	cell	445:448	arg1	structures					470:479	cell wall polysaccharide structures	445:479	cell wall polysaccharide structures (epitopes)	445:490	We provide an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant and marine algal materials and also describe treatments that can provide information on the masking of polysaccharides that may prevent detection.					
32617945	1	36	theme	Plant	130:134	arg1	composites					169:178	diverse composites	161:178	diverse composites of complex polysaccharides	161:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	1	36	theme	Plant	130:134	arg1	polysaccharides					191:205	complex polysaccharides	183:205	complex polysaccharides	183:205	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	1	36	theme	Plant	130:134	arg1	walls					151:155	Plant and algal cell walls	130:155	Plant and algal cell walls	130:155	Plant and algal cell walls are diverse composites of complex polysaccharides.					
32617945	0	37	theme	Polysaccharides	70:84	arg1	Antibodies					11:20	Monoclonal Antibodies	0:20	Monoclonal Antibodies	0:20	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	37	theme	Polysaccharides	70:84	arg1	Modules					44:50	Carbohydrate-Binding Modules	23:50	Carbohydrate-Binding Modules	23:50	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	37	theme	Polysaccharides	70:84	arg1	Detection					57:65	Detection	57:65	Detection of Polysaccharides in Cell Walls from Plants and Marine Algae	57:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	4	38	theme	important	785:793	arg1	aspect					795:800	an important aspect	782:800	an important aspect of cell wall immunocytochemistry	782:833	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	4	39	dep	wall	810:813	arg1	immunocytochemistry					815:833	immunocytochemistry	815:833	cell wall immunocytochemistry	805:833	These masking phenomena may indicate potential interactions between sets of cell wall polysaccharides and methods to uncover them are an important aspect of cell wall immunocytochemistry.					
32617945	0	40	from	Algae	123:127	arg1	Walls					94:98	Cell Walls	89:98	Cell Walls from Plants and Marine Algae	89:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	40	from	Algae	123:127	arg1	Antibodies					11:20	Monoclonal Antibodies	0:20	Monoclonal Antibodies	0:20	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	40	from	Algae	123:127	arg1	Modules					44:50	Carbohydrate-Binding Modules	23:50	Carbohydrate-Binding Modules	23:50	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	0	40	from	Algae	123:127	arg1	Detection					57:65	Detection	57:65	Detection of Polysaccharides in Cell Walls from Plants and Marine Algae	57:127	Monoclonal Antibodies, Carbohydrate-Binding Modules, and Detection of Polysaccharides in Cell Walls from Plants and Marine Algae.					
32617945	2	41	theme	wall	349:352	arg1	structures					354:363	cell wall structures	344:363	cell wall structures	344:363	Molecular probes such as monoclonal antibodies (MABs) and carbohydrate-binding modules (CBMs) are important tools to detect and dissect cell wall structures in these materials.					
33598924	0	0	theme	active	108:113	arg1	films					115:119	chitosan-Arthrospira platensis polysaccharide active films	62:119	chitosan-Arthrospira platensis polysaccharide active films	62:119	Preparation, and physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging.					
33598924	5	1	theme	X-ray	707:711	arg1	XRD					726:728	XRD	726:728	XRD	726:728	X-ray diffraction (XRD) patterns suggest the semi-crystalline structure was increased upon addition of APP.					
33598924	5	1	theme	X-ray	707:711	arg1	diffraction					713:723	X-ray diffraction	707:723	X-ray diffraction (XRD) patterns	707:738	X-ray diffraction (XRD) patterns suggest the semi-crystalline structure was increased upon addition of APP.					
33598924	6	2	from	%	939:939	arg1	degree					953:958	swelling degree	944:958	swelling degree	944:958	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	6	3	theme	composite	819:827	arg1	films					829:833	The composite films	815:833	The composite films	815:833	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	0	4	theme	polysaccharide	93:106	arg1	films					115:119	chitosan-Arthrospira platensis polysaccharide active films	62:119	chitosan-Arthrospira platensis polysaccharide active films	62:119	Preparation, and physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging.					
33598924	6	5	theme	water	854:858	arg1	resistance					860:869	an improved water resistance	842:869	an improved water resistance	842:869	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	1	6	theme	Arthrospira	186:196	arg1	APP					224:226	APP	224:226	APP	224:226	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	1	6	theme	Arthrospira	186:196	arg1	polysaccharide					208:221	Arthrospira platensis polysaccharide	186:221	Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v)	186:273	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	7	7	theme	spacing	1140:1146	arg1	shrinkage					1123:1131	the shrinkage	1119:1131	the shrinkage of the spacing of the polymer interchain	1119:1172	However, the transparency decreased slightly, which may be due to the shrinkage of the spacing of the polymer interchain.					
33598924	6	8	theme	improved	845:852	arg1	resistance					860:869	an improved water resistance	842:869	an improved water resistance	842:869	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	6	9	from	permeability	982:993	arg1	degree					953:958	swelling degree	944:958	swelling degree	944:958	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	0	10	theme	films	115:119	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, and physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging.					
33598924	0	10	theme	films	115:119	arg1	evaluation					48:57	physicochemical and biological evaluation	17:57	physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging	17:138	Preparation, and physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging.					
33598924	1	11	from	chitosan	159:166	arg1	films					148:152	Active films	141:152	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v)	141:273	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	8	12	theme	enhanced	1210:1217	arg1	properties					1234:1243	the mechanical properties	1219:1243	enhanced the mechanical properties	1210:1243	The composite films also displayed enhanced the mechanical properties.					
33598924	4	13	theme	atomic	669:674	arg1	AFM					694:696	AFM	694:696	AFM	694:696	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	4	13	theme	atomic	669:674	arg1	microscopy					682:691	atomic force microscopy	669:691	atomic force microscopy (AFM)	669:697	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	10	14	theme	CA-film	1434:1440	arg1	activity					1422:1429	the antioxidant activity	1406:1429	the antioxidant activity of CA-film	1406:1440	At a concentration of 1.2 mg/mL, the antioxidant activity of CA-film was enhanced by more than threefold compared with C-film.					
33598924	9	15	theme	zone	1346:1349	arg1	diameter					1351:1358	inhibition zone diameter	1335:1358	inhibition zone diameter for E. coli	1335:1370	The antimicrobial activity of the CA-film showed an increase of at least of 0.41-fold in inhibition zone diameter for E. coli.					
33598924	3	16	theme	infrared	491:498	arg1	FTIR					509:512	FTIR	509:512	FTIR	509:512	Fourier transform infrared spectra (FTIR) confirmed successful incorporation of chitosan and APP.					
33598924	3	16	theme	infrared	491:498	arg1	spectra					500:506	infrared spectra	491:506	infrared spectra (FTIR)	491:513	Fourier transform infrared spectra (FTIR) confirmed successful incorporation of chitosan and APP.					
33598924	4	17	theme	scanning	630:637	arg1	SEM					660:662	SEM	660:662	SEM	660:662	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	4	17	theme	scanning	630:637	arg1	microscopy					648:657	scanning electron microscopy	630:657	scanning electron microscopy (SEM)	630:663	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	1	18	theme	platensis	198:206	arg1	APP					224:226	APP	224:226	APP	224:226	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	1	18	theme	platensis	198:206	arg1	polysaccharide					208:221	Arthrospira platensis polysaccharide	186:221	Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v)	186:273	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	0	19	theme	food	125:128	arg1	packaging					130:138	food packaging	125:138	food packaging	125:138	Preparation, and physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging.					
33598924	11	20	theme	good	1525:1528	arg1	potential					1530:1538	good potential	1525:1538	good potential	1525:1538	Therefore, CA-films have good potential as sources of active packaging material for the food industry.					
33598924	11	20	theme	good	1525:1528	arg1	sources					1543:1549	sources	1543:1549	sources of active packaging material for the food industry	1543:1600	Therefore, CA-films have good potential as sources of active packaging material for the food industry.					
33598924	0	21	theme	biological	37:46	arg1	evaluation					48:57	physicochemical and biological evaluation	17:57	physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging	17:138	Preparation, and physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging.					
33598924	8	22	theme	mechanical	1223:1232	arg1	properties					1234:1243	the mechanical properties	1219:1243	enhanced the mechanical properties	1210:1243	The composite films also displayed enhanced the mechanical properties.					
33598924	5	23	theme	APP	810:812	arg1	addition					798:805	addition	798:805	addition of APP	798:812	X-ray diffraction (XRD) patterns suggest the semi-crystalline structure was increased upon addition of APP.					
33598924	5	24	theme	diffraction	713:723	arg1	patterns					731:738	X-ray diffraction (XRD) patterns	707:738	X-ray diffraction (XRD) patterns	707:738	X-ray diffraction (XRD) patterns suggest the semi-crystalline structure was increased upon addition of APP.					
33598924	7	25	theme	polymer	1155:1161	arg1	interchain					1163:1172	the polymer interchain	1151:1172	the polymer interchain	1151:1172	However, the transparency decreased slightly, which may be due to the shrinkage of the spacing of the polymer interchain.					
33598924	2	26	from	effect	315:320	arg1	properties					408:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties	332:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film)	332:453	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film) was investigated.					
33598924	3	27	theme	successful	525:534	arg1	incorporation					536:548	successful incorporation	525:548	successful incorporation of chitosan and APP	525:568	Fourier transform infrared spectra (FTIR) confirmed successful incorporation of chitosan and APP.					
33598924	6	28	theme	water	970:974	arg1	WVP					996:998	WVP	996:998	WVP	996:998	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	6	28	theme	water	970:974	arg1	permeability					982:993	water vapor permeability	970:993	water vapor permeability (WVP)	970:999	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	2	29	theme	structural	336:345	arg1	properties					408:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties	332:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film)	332:453	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film) was investigated.					
33598924	2	30	theme	antioxidant	396:406	arg1	properties					408:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties	332:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film)	332:453	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film) was investigated.					
33598924	6	31	from	%	929:929	arg1	degree					953:958	swelling degree	944:958	swelling degree	944:958	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	9	32	theme	antimicrobial	1250:1262	arg1	activity					1264:1271	The antimicrobial activity	1246:1271	The antimicrobial activity of the CA-film	1246:1286	The antimicrobial activity of the CA-film showed an increase of at least of 0.41-fold in inhibition zone diameter for E. coli.					
33598924	4	33	theme	microscopy	682:691	arg1	images					699:704	scanning electron microscopy (SEM) and atomic force microscopy (AFM) images	630:704	scanning electron microscopy (SEM) and atomic force microscopy (AFM) images	630:704	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	11	34	theme	material	1571:1578	arg1	potential					1530:1538	good potential	1525:1538	good potential	1525:1538	Therefore, CA-films have good potential as sources of active packaging material for the food industry.					
33598924	11	34	theme	material	1571:1578	arg1	sources					1543:1549	sources	1543:1549	sources of active packaging material for the food industry	1543:1600	Therefore, CA-films have good potential as sources of active packaging material for the food industry.					
33598924	0	35	theme	physicochemical	17:31	arg1	evaluation					48:57	physicochemical and biological evaluation	17:57	physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging	17:138	Preparation, and physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging.					
33598924	3	36	dep	Fourier	473:479	arg1	transform					481:489	transform	481:489	transform infrared spectra (FTIR)	481:513	Fourier transform infrared spectra (FTIR) confirmed successful incorporation of chitosan and APP.					
33598924	2	37	theme	antimicrobial	377:389	arg1	properties					408:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties	332:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film)	332:453	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film) was investigated.					
33598924	4	38	located	observed	610:617	arg2	structure					583:591	The compact structure	571:591	The compact structure of the films	571:604	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	4	38	located	observed	610:617	arg1	images					699:704	scanning electron microscopy (SEM) and atomic force microscopy (AFM) images	630:704	scanning electron microscopy (SEM) and atomic force microscopy (AFM) images	630:704	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	9	39	theme	at	1310:1311	arg1	increase					1298:1305	an increase	1295:1305	an increase of at least of 0.41-fold in inhibition zone diameter for E. coli	1295:1370	The antimicrobial activity of the CA-film showed an increase of at least of 0.41-fold in inhibition zone diameter for E. coli.					
33598924	5	40	theme	semi-crystalline	752:767	arg1	structure					769:777	the semi-crystalline structure	748:777	the semi-crystalline structure	748:777	X-ray diffraction (XRD) patterns suggest the semi-crystalline structure was increased upon addition of APP.					
33598924	2	41	theme	films	439:443	arg1	properties					408:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties	332:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film)	332:453	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film) was investigated.					
33598924	7	42	theme	interchain	1163:1172	arg1	spacing					1140:1146	the spacing	1136:1146	the spacing of the polymer interchain	1136:1172	However, the transparency decreased slightly, which may be due to the shrinkage of the spacing of the polymer interchain.					
33598924	1	43	theme	various	232:238	arg1	ratios					240:245	various ratios	232:245	various ratios (0.0%, 0.5%, and 1.0%, w/v)	232:273	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	2	44	theme	mechanical	365:374	arg1	properties					408:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties	332:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film)	332:453	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film) was investigated.					
33598924	2	45	theme	physicochemical	348:362	arg1	properties					408:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties	332:417	the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film)	332:453	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film) was investigated.					
33598924	2	46	theme	chitosan-APP	426:437	arg1	CA-film					446:452	CA-film	446:452	CA-film	446:452	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film) was investigated.					
33598924	2	46	theme	chitosan-APP	426:437	arg1	films					439:443	the chitosan-APP films	422:443	the chitosan-APP films (CA-film)	422:453	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film) was investigated.					
33598924	3	47	theme	APP	566:568	arg1	incorporation					536:548	successful incorporation	525:548	successful incorporation of chitosan and APP	525:568	Fourier transform infrared spectra (FTIR) confirmed successful incorporation of chitosan and APP.					
33598924	1	48	theme	ratios	240:245	arg1	APP					224:226	APP	224:226	APP	224:226	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	1	48	theme	ratios	240:245	arg1	polysaccharide					208:221	Arthrospira platensis polysaccharide	186:221	Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v)	186:273	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	11	49	theme	packaging	1561:1569	arg1	material					1571:1578	active packaging material	1554:1578	active packaging material	1554:1578	Therefore, CA-films have good potential as sources of active packaging material for the food industry.					
33598924	10	50	theme	1.2 mg/mL	1395:1403	arg1	concentration					1378:1390	a concentration	1376:1390	a concentration of 1.2 mg/mL	1376:1403	At a concentration of 1.2 mg/mL, the antioxidant activity of CA-film was enhanced by more than threefold compared with C-film.					
33598924	10	51	theme	antioxidant	1410:1420	arg1	activity					1422:1429	the antioxidant activity	1406:1429	the antioxidant activity of CA-film	1406:1440	At a concentration of 1.2 mg/mL, the antioxidant activity of CA-film was enhanced by more than threefold compared with C-film.					
33598924	8	52	theme	composite	1179:1187	arg1	films					1189:1193	The composite films	1175:1193	The composite films	1175:1193	The composite films also displayed enhanced the mechanical properties.					
33598924	9	53	from	increase	1298:1305	arg1	diameter					1351:1358	inhibition zone diameter	1335:1358	inhibition zone diameter for E. coli	1335:1370	The antimicrobial activity of the CA-film showed an increase of at least of 0.41-fold in inhibition zone diameter for E. coli.					
33598924	6	54	theme	barrier	881:887	arg1	properties					889:898	vapor barrier properties	875:898	vapor barrier properties	875:898	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	11	55	theme	food	1588:1591	arg1	industry					1593:1600	the food industry	1584:1600	the food industry	1584:1600	Therefore, CA-films have good potential as sources of active packaging material for the food industry.					
33598924	3	56	theme	chitosan	553:560	arg1	incorporation					536:548	successful incorporation	525:548	successful incorporation of chitosan and APP	525:568	Fourier transform infrared spectra (FTIR) confirmed successful incorporation of chitosan and APP.					
33598924	1	57	theme	Active	141:146	arg1	films					148:152	Active films	141:152	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v)	141:273	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	6	58	theme	vapor	875:879	arg1	properties					889:898	vapor barrier properties	875:898	vapor barrier properties	875:898	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	11	59	contain	have	1520:1523	arg1	CA-films					1511:1518	CA-films	1511:1518	CA-films	1511:1518	Therefore, CA-films have good potential as sources of active packaging material for the food industry.					
33598924	11	59	contain	have	1520:1523	arg2	sources					1543:1549	sources	1543:1549	sources of active packaging material for the food industry	1543:1600	Therefore, CA-films have good potential as sources of active packaging material for the food industry.					
33598924	11	59	contain	have	1520:1523	arg2	potential					1530:1538	good potential	1525:1538	good potential	1525:1538	Therefore, CA-films have good potential as sources of active packaging material for the food industry.					
33598924	4	60	theme	force	676:680	arg1	AFM					694:696	AFM	694:696	AFM	694:696	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	4	60	theme	force	676:680	arg1	microscopy					682:691	atomic force microscopy	669:691	atomic force microscopy (AFM)	669:697	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	6	61	theme	swelling	944:951	arg1	degree					953:958	swelling degree	944:958	swelling degree	944:958	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	1	62	dep	ratios	240:245	arg1	%					251:251	0.0%	248:251	0.0%	248:251	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	1	62	dep	ratios	240:245	arg1	%					257:257	0.5%	254:257	0.5%	254:257	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	1	62	dep	ratios	240:245	arg1	w/v					270:272	w/v	270:272	w/v	270:272	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	1	62	dep	ratios	240:245	arg1	%					267:267	1.0%	264:267	1.0%	264:267	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	4	63	theme	electron	639:646	arg1	SEM					660:662	SEM	660:662	SEM	660:662	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	4	63	theme	electron	639:646	arg1	microscopy					648:657	scanning electron microscopy	630:657	scanning electron microscopy (SEM)	630:663	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	6	64	theme	chitosan	1015:1022	arg1	C-film					1030:1035	C-film	1030:1035	C-film	1030:1035	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	6	64	theme	chitosan	1015:1022	arg1	film					1024:1027	chitosan film	1015:1027	chitosan film (C-film)	1015:1036	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	0	65	theme	platensis	83:91	arg1	films					115:119	chitosan-Arthrospira platensis polysaccharide active films	62:119	chitosan-Arthrospira platensis polysaccharide active films	62:119	Preparation, and physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging.					
33598924	4	66	theme	films	600:604	arg1	structure					583:591	The compact structure	571:591	The compact structure of the films	571:604	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	9	67	theme	inhibition	1335:1344	arg1	diameter					1351:1358	inhibition zone diameter	1335:1358	inhibition zone diameter for E. coli	1335:1370	The antimicrobial activity of the CA-film showed an increase of at least of 0.41-fold in inhibition zone diameter for E. coli.					
33598924	4	68	theme	microscopy	648:657	arg1	images					699:704	scanning electron microscopy (SEM) and atomic force microscopy (AFM) images	630:704	scanning electron microscopy (SEM) and atomic force microscopy (AFM) images	630:704	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33598924	6	69	theme	vapor	976:980	arg1	WVP					996:998	WVP	996:998	WVP	996:998	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	6	69	theme	vapor	976:980	arg1	permeability					982:993	water vapor permeability	970:993	water vapor permeability (WVP)	970:999	The composite films showed an improved water resistance and vapor barrier properties, and reduced by at least 27.4% and 32.1% in swelling degree (Sd ) and water vapor permeability (WVP) compared with chitosan film (C-film), respectively.					
33598924	9	70	theme	CA-film	1280:1286	arg1	activity					1264:1271	The antimicrobial activity	1246:1271	The antimicrobial activity of the CA-film	1246:1286	The antimicrobial activity of the CA-film showed an increase of at least of 0.41-fold in inhibition zone diameter for E. coli.					
33598924	0	71	theme	chitosan-Arthrospira	62:81	arg1	films					115:119	chitosan-Arthrospira platensis polysaccharide active films	62:119	chitosan-Arthrospira platensis polysaccharide active films	62:119	Preparation, and physicochemical and biological evaluation of chitosan-Arthrospira platensis polysaccharide active films for food packaging.					
33598924	1	72	theme	solution	293:300	arg1	casting					302:308	solution casting	293:308	solution casting	293:308	Active films from chitosan incorporated with Arthrospira platensis polysaccharide (APP) of various ratios (0.0%, 0.5%, and 1.0%, w/v) were developed by solution casting.					
33598924	2	73	theme	APP	325:327	arg1	effect					315:320	The effect	311:320	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film)	311:453	The effect of APP on the structural, physicochemical, mechanical, antimicrobial, and antioxidant properties of the chitosan-APP films (CA-film) was investigated.					
33598924	11	74	theme	active	1554:1559	arg1	material					1571:1578	active packaging material	1554:1578	active packaging material	1554:1578	Therefore, CA-films have good potential as sources of active packaging material for the food industry.					
33598924	4	75	theme	compact	575:581	arg1	structure					583:591	The compact structure	571:591	The compact structure of the films	571:604	The compact structure of the films was observed clearly in scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.					
33350816	0	0	theme	3D	95:96	arg1	Fabrication					98:108	3D Fabrication	95:108	3D Fabrication	95:108	Photocross-linkable Methacrylated Polypeptides and Polysaccharides for Casting, Injecting, and 3D Fabrication.					
33350816	8	1	theme	specific	1180:1187	arg1	method					1201:1206	the specific fabrication method	1176:1206	the specific fabrication method	1176:1206	These results underline the importance of tunability of the precursors and resulting hydrogels according to the specific fabrication method and tissue engineering application.					
33350816	8	2	theme	resulting	1143:1151	arg1	hydrogels					1153:1161	resulting hydrogels	1143:1161	resulting hydrogels	1143:1161	These results underline the importance of tunability of the precursors and resulting hydrogels according to the specific fabrication method and tissue engineering application.					
33350816	5	3	theme	collagen	651:658	arg1	precursors					637:646	The precursors	633:646	The precursors of collagen and gelatin with 60% methacrylation	633:694	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	7	4	theme	hydrogels	971:979	arg1	properties					957:966	the mechanical properties	942:966	the mechanical properties of hydrogels	942:979	Our study also showed that the mechanical properties of hydrogels corresponded to the used photocross-linking conditions and the degree of modification.					
33350816	5	5	with	collagen	651:658	arg1	methacrylation					681:694	60% methacrylation	677:694	60% methacrylation	677:694	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	8	6	dep	method	1201:1206	arg1	application					1231:1241	application	1231:1241	application	1231:1241	These results underline the importance of tunability of the precursors and resulting hydrogels according to the specific fabrication method and tissue engineering application.					
33350816	3	7	theme	modification	525:536	arg1	degree					515:520	30 and 60% degree	504:520	30 and 60% degree of modification	504:536	In this study, we successfully methacrylated collagen, gelatin, hyaluronan, and alginate to 30 and 60% degree of modification.					
33350816	3	8	dep	%	513:513	arg1	60					511:512	60	511:512	60	511:512	In this study, we successfully methacrylated collagen, gelatin, hyaluronan, and alginate to 30 and 60% degree of modification.					
33350816	3	8	dep	%	513:513	arg1	30					504:505	30	504:505	30	504:505	In this study, we successfully methacrylated collagen, gelatin, hyaluronan, and alginate to 30 and 60% degree of modification.					
33350816	5	9	theme	shear-thinning	729:742	arg1	properties					744:753	shear-thinning properties	729:753	shear-thinning properties	729:753	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	5	10	theme	gelatin	664:670	arg1	precursors					637:646	The precursors	633:646	The precursors of collagen and gelatin with 60% methacrylation	633:694	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	8	11	theme	tissue	1212:1217	arg1	engineering					1219:1229	tissue engineering	1212:1229	tissue engineering	1212:1229	These results underline the importance of tunability of the precursors and resulting hydrogels according to the specific fabrication method and tissue engineering application.					
33350816	3	12	dep	degree	515:520	arg1	%					513:513	%	513:513	%	513:513	In this study, we successfully methacrylated collagen, gelatin, hyaluronan, and alginate to 30 and 60% degree of modification.					
33350816	1	13	theme	new	167:169	arg1	hydrogels					171:179	new hydrogels	167:179	new hydrogels	167:179	For modern tissue engineering, we need not only develop new hydrogels but also suitable processing methods for them.					
33350816	0	14	theme	Methacrylated	20:32	arg1	Polypeptides					34:45	Photocross-linkable Methacrylated Polypeptides	0:45	Photocross-linkable Methacrylated Polypeptides	0:45	Photocross-linkable Methacrylated Polypeptides and Polysaccharides for Casting, Injecting, and 3D Fabrication.					
33350816	6	15	theme	suitable	884:891	arg1	properties					873:882	properties	873:882	properties suitable for casting purposes	873:912	On the contrary, the 30% methacrylated precursors had properties suitable for casting purposes.					
33350816	7	16	theme	mechanical	946:955	arg1	properties					957:966	the mechanical properties	942:966	the mechanical properties of hydrogels	942:979	Our study also showed that the mechanical properties of hydrogels corresponded to the used photocross-linking conditions and the degree of modification.					
33350816	6	17	theme	casting	897:903	arg1	purposes					905:912	casting purposes	897:912	casting purposes	897:912	On the contrary, the 30% methacrylated precursors had properties suitable for casting purposes.					
33350816	0	18	theme	Photocross-linkable	0:18	arg1	Polypeptides					34:45	Photocross-linkable Methacrylated Polypeptides	0:45	Photocross-linkable Methacrylated Polypeptides	0:45	Photocross-linkable Methacrylated Polypeptides and Polysaccharides for Casting, Injecting, and 3D Fabrication.					
33350816	8	19	theme	precursors	1128:1137	arg1	tunability					1110:1119	tunability	1110:1119	tunability of the precursors and resulting hydrogels	1110:1161	These results underline the importance of tunability of the precursors and resulting hydrogels according to the specific fabrication method and tissue engineering application.					
33350816	8	20	theme	tunability	1110:1119	arg1	importance					1096:1105	the importance	1092:1105	the importance of tunability of the precursors and resulting hydrogels	1092:1161	These results underline the importance of tunability of the precursors and resulting hydrogels according to the specific fabrication method and tissue engineering application.					
33350816	1	21	theme	suitable	190:197	arg1	methods					210:216	suitable processing methods	190:216	suitable processing methods	190:216	For modern tissue engineering, we need not only develop new hydrogels but also suitable processing methods for them.					
33350816	5	22	theme	suitable	706:713	arg1	stress					721:726	suitable yield stress	706:726	suitable yield stress	706:726	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	4	23	dep	precursors	584:593	arg1	i.e.					578:581	i.e.	578:581	i.e.	578:581	We studied methacrylated compositions (i.e., precursors) to investigate their processability.					
33350816	1	24	theme	modern	115:120	arg1	engineering					129:139	modern tissue engineering	115:139	modern tissue engineering	115:139	For modern tissue engineering, we need not only develop new hydrogels but also suitable processing methods for them.					
33350816	1	25	theme	processing	199:208	arg1	methods					210:216	suitable processing methods	190:216	suitable processing methods	190:216	For modern tissue engineering, we need not only develop new hydrogels but also suitable processing methods for them.					
33350816	4	26	theme	methacrylated	550:562	arg1	precursors					584:593	precursors	584:593	precursors	584:593	We studied methacrylated compositions (i.e., precursors) to investigate their processability.					
33350816	4	26	theme	methacrylated	550:562	arg1	compositions					564:575	methacrylated compositions	550:575	methacrylated compositions (i.e., precursors)	550:594	We studied methacrylated compositions (i.e., precursors) to investigate their processability.					
33350816	5	27	theme	yield	715:719	arg1	stress					721:726	suitable yield stress	706:726	suitable yield stress	706:726	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	5	28	theme	3D	803:804	arg1	bioprinting					806:816	3D bioprinting	803:816	3D bioprinting	803:816	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	6	29	theme	methacrylated	844:856	arg1	precursors					858:867	the 30% methacrylated precursors	836:867	the 30% methacrylated precursors	836:867	On the contrary, the 30% methacrylated precursors had properties suitable for casting purposes.					
33350816	8	30	theme	hydrogels	1153:1161	arg1	tunability					1110:1119	tunability	1110:1119	tunability of the precursors and resulting hydrogels	1110:1161	These results underline the importance of tunability of the precursors and resulting hydrogels according to the specific fabrication method and tissue engineering application.					
33350816	1	31	theme	tissue	122:127	arg1	engineering					129:139	modern tissue engineering	115:139	modern tissue engineering	115:139	For modern tissue engineering, we need not only develop new hydrogels but also suitable processing methods for them.					
33350816	5	32	theme	60	677:678	arg1	%					679:679	%	679:679	%	679:679	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	6	33	theme	%	842:842	arg1	precursors					858:867	the 30% methacrylated precursors	836:867	the 30% methacrylated precursors	836:867	On the contrary, the 30% methacrylated precursors had properties suitable for casting purposes.					
33350816	5	34	theme	%	679:679	arg1	methacrylation					681:694	60% methacrylation	677:694	60% methacrylation	677:694	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	6	35	theme	30	840:841	arg1	%					842:842	%	842:842	%	842:842	On the contrary, the 30% methacrylated precursors had properties suitable for casting purposes.					
33350816	6	36	contain	had	869:871	arg2	properties					873:882	properties	873:882	properties suitable for casting purposes	873:912	On the contrary, the 30% methacrylated precursors had properties suitable for casting purposes.					
33350816	6	36	contain	had	869:871	arg1	precursors					858:867	the 30% methacrylated precursors	836:867	the 30% methacrylated precursors	836:867	On the contrary, the 30% methacrylated precursors had properties suitable for casting purposes.					
33350816	7	37	theme	modification	1054:1065	arg1	conditions					1025:1034	the used photocross-linking conditions	997:1034	the used photocross-linking conditions	997:1034	Our study also showed that the mechanical properties of hydrogels corresponded to the used photocross-linking conditions and the degree of modification.					
33350816	7	37	theme	modification	1054:1065	arg1	degree					1044:1049	the degree	1040:1049	the degree of modification	1040:1065	Our study also showed that the mechanical properties of hydrogels corresponded to the used photocross-linking conditions and the degree of modification.					
33350816	2	38	theme	potential	265:273	arg1	Polypeptides					228:239	Polypeptides	228:239	Polypeptides	228:239	Polypeptides and polysaccharides are potential candidates because they can be methacrylated, processed before photocross-linking, and yielded into hydrogels with given shape and form.					
33350816	2	38	theme	potential	265:273	arg1	polysaccharides					245:259	polysaccharides	245:259	polysaccharides	245:259	Polypeptides and polysaccharides are potential candidates because they can be methacrylated, processed before photocross-linking, and yielded into hydrogels with given shape and form.					
33350816	2	38	theme	potential	265:273	arg1	candidates					275:284	potential candidates	265:284	potential candidates	265:284	Polypeptides and polysaccharides are potential candidates because they can be methacrylated, processed before photocross-linking, and yielded into hydrogels with given shape and form.					
33350816	7	39	theme	used	1001:1004	arg1	conditions					1025:1034	the used photocross-linking conditions	997:1034	the used photocross-linking conditions	997:1034	Our study also showed that the mechanical properties of hydrogels corresponded to the used photocross-linking conditions and the degree of modification.					
33350816	7	40	theme	photocross-linking	1006:1023	arg1	conditions					1025:1034	the used photocross-linking conditions	997:1034	the used photocross-linking conditions	997:1034	Our study also showed that the mechanical properties of hydrogels corresponded to the used photocross-linking conditions and the degree of modification.					
33350816	5	41	theme	fiber-forming	760:772	arg1	capability					774:783	fiber-forming capability	760:783	fiber-forming capability	760:783	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	5	42	with	gelatin	664:670	arg1	methacrylation					681:694	60% methacrylation	677:694	60% methacrylation	677:694	The precursors of collagen and gelatin with 60% methacrylation exhibited suitable yield stress, shear-thinning properties, and fiber-forming capability for injecting and 3D bioprinting.					
33350816	8	43	theme	fabrication	1189:1199	arg1	method					1201:1206	the specific fabrication method	1176:1206	the specific fabrication method	1176:1206	These results underline the importance of tunability of the precursors and resulting hydrogels according to the specific fabrication method and tissue engineering application.					
33887288	2	0	theme	mg/kg	446:450	arg1	b.w.					452:455	282 mg/kg b.w.	442:455	282 mg/kg b.w.	442:455	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	2	0	theme	mg/kg	446:450	arg1	oyster					428:433	steamed oyster	420:433	steamed oyster (SPS) (282 mg/kg b.w.)	420:456	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	8	1	theme	steaming	1192:1199	arg1	process					1201:1207	the steaming process	1188:1207	the steaming process	1188:1207	These results indicated that oyster polysaccharides alleviated alcoholic liver injury by mediating the gut-liver-metabolite axis, and the steaming process had little influence on the bioactivity.					
33887288	2	2	theme	282	442:444	arg1	b.w.					452:455	282 mg/kg b.w.	442:455	282 mg/kg b.w.	442:455	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	2	2	theme	282	442:444	arg1	oyster					428:433	steamed oyster	420:433	steamed oyster (SPS) (282 mg/kg b.w.)	420:456	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	2	3	from	oyster	428:433	arg1	polysaccharides					399:413	polysaccharides	399:413	polysaccharides	399:413	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	2	3	from	oyster	428:433	arg1	RPS					392:394	RPS	392:394	RPS	392:394	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	2	3	from	oyster	428:433	arg1	administration					374:387	Oral administration	369:387	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.)	369:456	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	4	4	theme	SPS	679:681	arg1	treatments					683:692	RPS and SPS treatments	671:692	RPS and SPS treatments	671:692	RPS and SPS treatments also significantly increased Lactobacillus reuteri and Roseburia spp.					
33887288	1	5	theme	Crassostrea	236:246	arg1	RPS					255:257	RPS	255:257	RPS	255:257	The objective of this study was to evaluate the molecular mechanism by which polysaccharides from Crassostrea gigas (RPS) prevent alcoholic liver injury and to uncover whether the steaming process affects the bioactivities of RPS.					
33887288	1	5	theme	Crassostrea	236:246	arg1	gigas					248:252	Crassostrea gigas	236:252	Crassostrea gigas (RPS)	236:258	The objective of this study was to evaluate the molecular mechanism by which polysaccharides from Crassostrea gigas (RPS) prevent alcoholic liver injury and to uncover whether the steaming process affects the bioactivities of RPS.					
33887288	2	6	theme	liver	493:497	arg1	injury					499:504	alcoholic liver injury	483:504	alcoholic liver injury in mice	483:512	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	1	7	theme	steaming	318:325	arg1	process					327:333	the steaming process	314:333	the steaming process	314:333	The objective of this study was to evaluate the molecular mechanism by which polysaccharides from Crassostrea gigas (RPS) prevent alcoholic liver injury and to uncover whether the steaming process affects the bioactivities of RPS.					
33887288	7	8	theme	SPS	982:984	arg1	effects					963:969	the beneficial effects	948:969	the beneficial effects of RPS and SPS	948:984	Correlation analysis revealed that the beneficial effects of RPS and SPS were strongly correlated with the microbiota composition and SCFAs.					
33887288	8	9	contain	had	1209:1211	arg1	process					1201:1207	the steaming process	1188:1207	the steaming process	1188:1207	These results indicated that oyster polysaccharides alleviated alcoholic liver injury by mediating the gut-liver-metabolite axis, and the steaming process had little influence on the bioactivity.					
33887288	8	9	contain	had	1209:1211	arg2	influence					1220:1228	little influence	1213:1228	little influence	1213:1228	These results indicated that oyster polysaccharides alleviated alcoholic liver injury by mediating the gut-liver-metabolite axis, and the steaming process had little influence on the bioactivity.					
33887288	2	10	theme	alcoholic	483:491	arg1	injury					499:504	alcoholic liver injury	483:504	alcoholic liver injury in mice	483:512	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	0	11	theme	protective	95:104	arg1	role					106:109	A possible protective role	84:109	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.	0:136	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
33887288	6	12	theme	SPS-treated	895:905	arg1	mice					907:910	RPS- and SPS-treated mice	886:910	RPS- and SPS-treated mice	886:910	Microbial metabolites, especially propionate and butyrate, were also increased in RPS- and SPS-treated mice.					
33887288	6	13	theme	Microbial	804:812	arg1	butyrate					853:860	butyrate	853:860	butyrate	853:860	Microbial metabolites, especially propionate and butyrate, were also increased in RPS- and SPS-treated mice.					
33887288	6	13	theme	Microbial	804:812	arg1	metabolites					814:824	Microbial metabolites	804:824	Microbial metabolites	804:824	Microbial metabolites, especially propionate and butyrate, were also increased in RPS- and SPS-treated mice.					
33887288	6	13	theme	Microbial	804:812	arg1	propionate					838:847	propionate	838:847	propionate	838:847	Microbial metabolites, especially propionate and butyrate, were also increased in RPS- and SPS-treated mice.					
33887288	2	14	theme	Oral	369:372	arg1	administration					374:387	Oral administration	369:387	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.)	369:456	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	0	15	theme	possible	86:93	arg1	role					106:109	A possible protective role	84:109	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.	0:136	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
33887288	7	16	theme	beneficial	952:961	arg1	effects					963:969	the beneficial effects	948:969	the beneficial effects of RPS and SPS	948:984	Correlation analysis revealed that the beneficial effects of RPS and SPS were strongly correlated with the microbiota composition and SCFAs.					
33887288	8	17	theme	little	1213:1218	arg1	influence					1220:1228	little influence	1213:1228	little influence	1213:1228	These results indicated that oyster polysaccharides alleviated alcoholic liver injury by mediating the gut-liver-metabolite axis, and the steaming process had little influence on the bioactivity.					
33887288	6	18	theme	RPS-	886:889	arg1	mice					907:910	RPS- and SPS-treated mice	886:910	RPS- and SPS-treated mice	886:910	Microbial metabolites, especially propionate and butyrate, were also increased in RPS- and SPS-treated mice.					
33887288	4	19	dep	Lactobacillus	723:735	arg1	reuteri					737:743	reuteri	737:743	reuteri	737:743	RPS and SPS treatments also significantly increased Lactobacillus reuteri and Roseburia spp.					
33887288	1	20	theme	study	160:164	arg1	objective					142:150	The objective	138:150	The objective of this study	138:164	The objective of this study was to evaluate the molecular mechanism by which polysaccharides from Crassostrea gigas (RPS) prevent alcoholic liver injury and to uncover whether the steaming process affects the bioactivities of RPS.					
33887288	2	21	theme	steamed	420:426	arg1	b.w.					452:455	282 mg/kg b.w.	442:455	282 mg/kg b.w.	442:455	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	2	21	theme	steamed	420:426	arg1	SPS					436:438	SPS	436:438	SPS	436:438	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	2	21	theme	steamed	420:426	arg1	oyster					428:433	steamed oyster	420:433	steamed oyster (SPS) (282 mg/kg b.w.)	420:456	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	8	22	theme	oyster	1083:1088	arg1	polysaccharides					1090:1104	oyster polysaccharides	1083:1104	oyster polysaccharides	1083:1104	These results indicated that oyster polysaccharides alleviated alcoholic liver injury by mediating the gut-liver-metabolite axis, and the steaming process had little influence on the bioactivity.					
33887288	0	23	theme	microbiome-gut	17:30	arg1	axis					32:35	the microbiome-gut axis	13:35	the microbiome-gut axis	13:35	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
33887288	1	24	from	gigas	248:252	arg1	polysaccharides					215:229	polysaccharides	215:229	polysaccharides from Crassostrea gigas (RPS)	215:258	The objective of this study was to evaluate the molecular mechanism by which polysaccharides from Crassostrea gigas (RPS) prevent alcoholic liver injury and to uncover whether the steaming process affects the bioactivities of RPS.					
33887288	7	25	theme	RPS	974:976	arg1	effects					963:969	the beneficial effects	948:969	the beneficial effects of RPS and SPS	948:984	Correlation analysis revealed that the beneficial effects of RPS and SPS were strongly correlated with the microbiota composition and SCFAs.					
33887288	2	26	theme	polysaccharides	399:413	arg1	administration					374:387	Oral administration	369:387	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.)	369:456	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	3	27	theme	proteins	622:629	arg1	expression					593:602	the expression	589:602	the expression of tight-junction proteins	589:629	RPS and SPS treatments protected gut functions by significantly enhancing the expression of tight-junction proteins and suppressing inflammatory responses.					
33887288	2	28	theme	RPS	392:394	arg1	administration					374:387	Oral administration	369:387	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.)	369:456	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	1	29	theme	alcoholic	268:276	arg1	injury					284:289	alcoholic liver injury	268:289	alcoholic liver injury	268:289	The objective of this study was to evaluate the molecular mechanism by which polysaccharides from Crassostrea gigas (RPS) prevent alcoholic liver injury and to uncover whether the steaming process affects the bioactivities of RPS.					
33887288	0	30	theme	liver	124:128	arg1	injury					130:135	alcoholic liver injury	114:135	alcoholic liver injury	114:135	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
33887288	1	31	theme	RPS	364:366	arg1	bioactivities					347:359	the bioactivities	343:359	the bioactivities of RPS	343:366	The objective of this study was to evaluate the molecular mechanism by which polysaccharides from Crassostrea gigas (RPS) prevent alcoholic liver injury and to uncover whether the steaming process affects the bioactivities of RPS.					
33887288	0	32	theme	axis	32:35	arg1	Mediation					0:8	Mediation	0:8	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.	0:136	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
33887288	1	33	theme	liver	278:282	arg1	injury					284:289	alcoholic liver injury	268:289	alcoholic liver injury	268:289	The objective of this study was to evaluate the molecular mechanism by which polysaccharides from Crassostrea gigas (RPS) prevent alcoholic liver injury and to uncover whether the steaming process affects the bioactivities of RPS.					
33887288	0	34	theme	alcoholic	114:122	arg1	injury					130:135	alcoholic liver injury	114:135	alcoholic liver injury	114:135	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
33887288	1	35	theme	molecular	186:194	arg1	mechanism					196:204	the molecular mechanism	182:204	the molecular mechanism by which polysaccharides from Crassostrea gigas (RPS) prevent alcoholic liver injury	182:289	The objective of this study was to evaluate the molecular mechanism by which polysaccharides from Crassostrea gigas (RPS) prevent alcoholic liver injury and to uncover whether the steaming process affects the bioactivities of RPS.					
33887288	5	36	theme	Escherichia	791:801	arg1	level					782:786	the level	778:786	the level of Escherichia	778:801	and decreased the level of Escherichia.					
33887288	3	37	theme	tight-junction	607:620	arg1	proteins					622:629	tight-junction proteins	607:629	tight-junction proteins	607:629	RPS and SPS treatments protected gut functions by significantly enhancing the expression of tight-junction proteins and suppressing inflammatory responses.					
33887288	0	38	theme	oyster	40:45	arg1	polysaccharides					67:81	oyster (Crassostrea gigas) polysaccharides	40:81	oyster (Crassostrea gigas) polysaccharides	40:81	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
33887288	8	39	theme	alcoholic	1117:1125	arg1	injury					1133:1138	alcoholic liver injury	1117:1138	alcoholic liver injury	1117:1138	These results indicated that oyster polysaccharides alleviated alcoholic liver injury by mediating the gut-liver-metabolite axis, and the steaming process had little influence on the bioactivity.					
33887288	3	40	theme	gut	548:550	arg1	functions					552:560	gut functions	548:560	gut functions	548:560	RPS and SPS treatments protected gut functions by significantly enhancing the expression of tight-junction proteins and suppressing inflammatory responses.					
33887288	8	41	theme	gut-liver-metabolite	1157:1176	arg1	axis					1178:1181	the gut-liver-metabolite axis	1153:1181	the gut-liver-metabolite axis	1153:1181	These results indicated that oyster polysaccharides alleviated alcoholic liver injury by mediating the gut-liver-metabolite axis, and the steaming process had little influence on the bioactivity.					
33887288	7	42	theme	microbiota	1020:1029	arg1	composition					1031:1041	the microbiota composition	1016:1041	the microbiota composition	1016:1041	Correlation analysis revealed that the beneficial effects of RPS and SPS were strongly correlated with the microbiota composition and SCFAs.					
33887288	2	43	from	injury	499:504	arg1	mice					509:512	mice	509:512	mice	509:512	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	3	44	theme	inflammatory	647:658	arg1	responses					660:668	inflammatory responses	647:668	inflammatory responses	647:668	RPS and SPS treatments protected gut functions by significantly enhancing the expression of tight-junction proteins and suppressing inflammatory responses.					
33887288	2	45	from	administration	374:387	arg1	b.w.					452:455	282 mg/kg b.w.	442:455	282 mg/kg b.w.	442:455	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	2	45	from	administration	374:387	arg1	SPS					436:438	SPS	436:438	SPS	436:438	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	2	45	from	administration	374:387	arg1	oyster					428:433	steamed oyster	420:433	steamed oyster (SPS) (282 mg/kg b.w.)	420:456	Oral administration of RPS or polysaccharides from steamed oyster (SPS) (282 mg/kg b.w.) significantly attenuated alcoholic liver injury in mice.					
33887288	3	46	dep	RPS	515:517	arg1	treatments					527:536	treatments	527:536	treatments	527:536	RPS and SPS treatments protected gut functions by significantly enhancing the expression of tight-junction proteins and suppressing inflammatory responses.					
33887288	7	47	theme	Correlation	913:923	arg1	analysis					925:932	Correlation analysis	913:932	Correlation analysis	913:932	Correlation analysis revealed that the beneficial effects of RPS and SPS were strongly correlated with the microbiota composition and SCFAs.					
33887288	0	48	theme	Crassostrea	48:58	arg1	oyster					40:45	oyster	40:45	oyster (Crassostrea gigas) polysaccharides	40:81	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
33887288	0	48	theme	Crassostrea	48:58	arg1	gigas					60:64	Crassostrea gigas	48:64	Crassostrea gigas	48:64	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
33887288	0	49	dep	Mediation	0:8	arg1	role					106:109	A possible protective role	84:109	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.	0:136	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
33887288	4	50	theme	RPS	671:673	arg1	treatments					683:692	RPS and SPS treatments	671:692	RPS and SPS treatments	671:692	RPS and SPS treatments also significantly increased Lactobacillus reuteri and Roseburia spp.					
33887288	8	51	theme	liver	1127:1131	arg1	injury					1133:1138	alcoholic liver injury	1117:1138	alcoholic liver injury	1117:1138	These results indicated that oyster polysaccharides alleviated alcoholic liver injury by mediating the gut-liver-metabolite axis, and the steaming process had little influence on the bioactivity.					
33887288	0	52	from	role	106:109	arg1	injury					130:135	alcoholic liver injury	114:135	alcoholic liver injury	114:135	Mediation of the microbiome-gut axis by oyster (Crassostrea gigas) polysaccharides: A possible protective role in alcoholic liver injury.					
32103179	6	0	theme	acceptor	1159:1166	arg1	substrates					1168:1177	donor and acceptor substrates	1149:1177	donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro	1149:1265	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	6	1	theme	ALG	1209:1211	arg1	pathway					1213:1219	the ALG pathway	1205:1219	the ALG pathway	1205:1219	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	4	2	from	resolution	748:757	arg1	structure					715:723	the cryo-electron microscopy structure	686:723	the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold	686:824	Here we present the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold.					
32103179	6	3	theme	enzymatic	1110:1118	arg1	extension					1127:1135	enzymatic glycan extension	1110:1135	enzymatic glycan extension	1110:1135	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	5	4	theme	reported	843:850	arg1	structures					857:866	reported GT-C structures	843:866	reported GT-C structures	843:866	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	6	5	theme	pathway	1213:1219	arg1	enzymes					1194:1200	purified enzymes	1185:1200	purified enzymes of the ALG pathway	1185:1219	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	1	6	theme	dolichylpyrophosphate-linked	169:196	arg1	oligosaccharide					198:212	a dolichylpyrophosphate-linked oligosaccharide	167:212	a dolichylpyrophosphate-linked oligosaccharide	167:212	In eukaryotic protein N-glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its transfer onto acceptor proteins1.					
32103179	7	7	theme	ALG6	1315:1318	arg1	structure					1302:1310	A second cryo-electron microscopy structure	1268:1310	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution	1268:1388	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution revealed the active site of the enzyme.					
32103179	8	8	theme	general	1532:1538	arg1	residue					1500:1506	a catalytic aspartate residue	1478:1506	a catalytic aspartate residue that probably acts as a general base	1478:1543	Functional analysis of ALG6 variants identified a catalytic aspartate residue that probably acts as a general base.					
32103179	8	8	theme	general	1532:1538	arg1	base					1540:1543	a general base	1530:1543	a general base	1530:1543	Functional analysis of ALG6 variants identified a catalytic aspartate residue that probably acts as a general base.					
32103179	2	9	theme	reticulum	319:327	arg1	lumen					294:298	the lumen	290:298	the lumen of the endoplasmic reticulum (ER)	290:332	The final seven steps occur in the lumen of the endoplasmic reticulum (ER) and require dolichylphosphate-activated mannose and glucose as donor substrates2.					
32103179	6	10	theme	ALG6	1253:1256	arg1	activity					1241:1248	the activity	1237:1248	the activity of ALG6	1237:1256	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	6	11	theme	sugars	1099:1104	arg1	extension					1127:1135	enzymatic glycan extension	1110:1135	enzymatic glycan extension	1110:1135	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	6	11	theme	sugars	1099:1104	arg1	analogues					1028:1036	synthetic analogues	1018:1036	synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars	1018:1104	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	3	12	dep	enzymes-ALG3	432:443	arg1	substrate3,4					656:667	an isoprenoid-linked carbohydrate donor substrate3,4	616:667	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.	416:668	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	8	13	theme	variants	1458:1465	arg1	analysis					1441:1448	Functional analysis	1430:1448	Functional analysis of ALG6 variants	1430:1465	Functional analysis of ALG6 variants identified a catalytic aspartate residue that probably acts as a general base.					
32103179	2	14	theme	endoplasmic	307:317	arg1	ER					330:331	ER	330:331	ER	330:331	The final seven steps occur in the lumen of the endoplasmic reticulum (ER) and require dolichylphosphate-activated mannose and glucose as donor substrates2.					
32103179	2	14	theme	endoplasmic	307:317	arg1	reticulum					319:327	the endoplasmic reticulum	303:327	the endoplasmic reticulum (ER)	303:332	The final seven steps occur in the lumen of the endoplasmic reticulum (ER) and require dolichylphosphate-activated mannose and glucose as donor substrates2.					
32103179	9	15	theme	GT-C	1579:1582	arg1	superfamily					1584:1594	the GT-C superfamily	1575:1594	the GT-C superfamily	1575:1594	This residue is conserved in the GT-C superfamily.					
32103179	5	16	theme	conserved	933:941	arg1	module					943:948	a conserved module	931:948	a conserved module	931:948	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	5	16	theme	conserved	933:941	arg1	each					973:976	each	973:976	each	973:976	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	7	17	theme	3.9 Å	1373:1377	arg1	resolution					1379:1388	3.9 Å resolution	1373:1388	3.9 Å resolution	1373:1388	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution revealed the active site of the enzyme.					
32103179	7	18	theme	second	1270:1275	arg1	structure					1302:1310	A second cryo-electron microscopy structure	1268:1310	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution	1268:1388	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution revealed the active site of the enzyme.					
32103179	5	19	theme	GT-C	882:885	arg1	enzymes					887:893	GT-C enzymes	882:893	GT-C enzymes	882:893	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	1	20	theme	eukaryotic	69:78	arg1	N-glycosylation					88:102	eukaryotic protein N-glycosylation	69:102	eukaryotic protein N-glycosylation	69:102	In eukaryotic protein N-glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its transfer onto acceptor proteins1.					
32103179	6	21	theme	purified	1185:1192	arg1	enzymes					1194:1200	purified enzymes	1185:1200	purified enzymes of the ALG pathway	1185:1219	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	1	22	theme	oligosaccharide	198:212	arg1	biosynthesis					151:162	the biosynthesis	147:162	the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide	147:212	In eukaryotic protein N-glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its transfer onto acceptor proteins1.					
32103179	6	23	theme	dolichylpyrophosphate-linked	1070:1097	arg1	sugars					1099:1104	dolichylphosphate-linked and dolichylpyrophosphate-linked sugars	1041:1104	dolichylphosphate-linked and dolichylpyrophosphate-linked sugars	1041:1104	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	5	24	theme	GT-C	852:855	arg1	structures					857:866	reported GT-C structures	843:866	reported GT-C structures	843:866	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	1	25	theme	protein	80:86	arg1	N-glycosylation					88:102	eukaryotic protein N-glycosylation	69:102	eukaryotic protein N-glycosylation	69:102	In eukaryotic protein N-glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its transfer onto acceptor proteins1.					
32103179	3	26	theme	responsible	420:430	arg1	enzymes-ALG3					432:443	The responsible enzymes-ALG3	416:443	The responsible enzymes-ALG3	416:443	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	4	27	theme	transmembrane	799:811	arg1	fold					821:824	a previously undescribed transmembrane protein fold	774:824	a previously undescribed transmembrane protein fold	774:824	Here we present the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold.					
32103179	7	28	theme	cryo-electron	1277:1289	arg1	microscopy					1291:1300	cryo-electron microscopy	1277:1300	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution	1268:1388	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution revealed the active site of the enzyme.					
32103179	4	29	theme	ALG6	734:737	arg1	structure					715:723	the cryo-electron microscopy structure	686:723	the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold	686:824	Here we present the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold.					
32103179	7	30	theme	microscopy	1291:1300	arg1	structure					1302:1310	A second cryo-electron microscopy structure	1268:1310	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution	1268:1388	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution revealed the active site of the enzyme.					
32103179	4	31	theme	undescribed	787:797	arg1	fold					821:824	a previously undescribed transmembrane protein fold	774:824	a previously undescribed transmembrane protein fold	774:824	Here we present the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold.					
32103179	8	32	theme	catalytic	1480:1488	arg1	residue					1500:1506	a catalytic aspartate residue	1478:1506	a catalytic aspartate residue that probably acts as a general base	1478:1543	Functional analysis of ALG6 variants identified a catalytic aspartate residue that probably acts as a general base.					
32103179	8	32	theme	catalytic	1480:1488	arg1	base					1540:1543	a general base	1530:1543	a general base	1530:1543	Functional analysis of ALG6 variants identified a catalytic aspartate residue that probably acts as a general base.					
32103179	4	33	theme	yeast	728:732	arg1	ALG6					734:737	yeast ALG6	728:737	yeast ALG6	728:737	Here we present the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold.					
32103179	6	34	theme	dolichylphosphate-linked	1041:1064	arg1	sugars					1099:1104	dolichylphosphate-linked and dolichylpyrophosphate-linked sugars	1041:1104	dolichylphosphate-linked and dolichylpyrophosphate-linked sugars	1041:1104	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	6	35	link	dolichylphosphate-linked	1041:1064	arg1	sugars					1099:1104	dolichylphosphate-linked and dolichylpyrophosphate-linked sugars	1041:1104	dolichylphosphate-linked and dolichylpyrophosphate-linked sugars	1041:1104	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	3	36	theme	membrane-spanning	575:591	arg1	helices					593:599	membrane-spanning helices	575:599	membrane-spanning helices	575:599	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	5	37	theme	modular	905:911	arg1	architecture					913:924	a modular architecture	903:924	a modular architecture with a conserved module and a variable module, each with distinct functional roles	903:1007	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	6	38	link	dolichylpyrophosphate-linked	1070:1097	arg1	sugars					1099:1104	dolichylphosphate-linked and dolichylpyrophosphate-linked sugars	1041:1104	dolichylphosphate-linked and dolichylpyrophosphate-linked sugars	1041:1104	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	10	39	theme	enzymes	1652:1658	arg1	architecture					1620:1631	the architecture	1616:1631	the architecture of ER-luminal GT-C enzymes	1616:1658	Our results define the architecture of ER-luminal GT-C enzymes and provide a structural basis for understanding their catalytic mechanisms.					
32103179	1	40	link	dolichylpyrophosphate-linked	169:196	arg1	oligosaccharide					198:212	a dolichylpyrophosphate-linked oligosaccharide	167:212	a dolichylpyrophosphate-linked oligosaccharide	167:212	In eukaryotic protein N-glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its transfer onto acceptor proteins1.					
32103179	4	41	theme	protein	813:819	arg1	fold					821:824	a previously undescribed transmembrane protein fold	774:824	a previously undescribed transmembrane protein fold	774:824	Here we present the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold.					
32103179	1	42	theme	acceptor	239:246	arg1	proteins1					248:256	acceptor proteins1	239:256	acceptor proteins1	239:256	In eukaryotic protein N-glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its transfer onto acceptor proteins1.					
32103179	0	43	theme	ER-based	31:38	arg1	ALG6					60:63	the ER-based glucosyltransferase ALG6	27:63	the ER-based glucosyltransferase ALG6	27:63	Structure and mechanism of the ER-based glucosyltransferase ALG6.					
32103179	5	44	theme	distinct	983:990	arg1	roles					1003:1007	distinct functional roles	983:1007	distinct functional roles	983:1007	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	6	45	used	used	1013:1016	arg2	We					1010:1011	We	1010:1011	We	1010:1011	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	5	46	contain	contain	895:901	arg1	enzymes					887:893	GT-C enzymes	882:893	GT-C enzymes	882:893	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	5	46	contain	contain	895:901	arg2	architecture					913:924	a modular architecture	903:924	a modular architecture with a conserved module and a variable module, each with distinct functional roles	903:1007	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	3	47	theme	carbohydrate	637:648	arg1	substrate3,4					656:667	an isoprenoid-linked carbohydrate donor substrate3,4	616:667	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.	416:668	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	4	48	theme	microscopy	704:713	arg1	structure					715:723	the cryo-electron microscopy structure	686:723	the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold	686:824	Here we present the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold.					
32103179	3	49	link	isoprenoid-linked	619:635	arg1	substrate3,4					656:667	an isoprenoid-linked carbohydrate donor substrate3,4	616:667	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.	416:668	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	6	50	theme	synthetic	1018:1026	arg1	analogues					1028:1036	synthetic analogues	1018:1036	synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars	1018:1104	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	5	51	with	architecture	913:924	arg1	module					943:948	a conserved module	931:948	a conserved module	931:948	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	5	51	with	architecture	913:924	arg1	each					973:976	each	973:976	each	973:976	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	5	51	with	architecture	913:924	arg1	module					965:970	a variable module	954:970	a variable module	954:970	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	8	52	theme	Functional	1430:1439	arg1	analysis					1441:1448	Functional analysis	1430:1448	Functional analysis of ALG6 variants	1430:1465	Functional analysis of ALG6 variants identified a catalytic aspartate residue that probably acts as a general base.					
32103179	3	53	theme	donor	650:654	arg1	substrate3,4					656:667	an isoprenoid-linked carbohydrate donor substrate3,4	616:667	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.	416:668	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	3	54	theme	ALG10-are	474:482	arg1	glycosyltransferases					484:503	ALG10-are glycosyltransferases	474:503	ALG10-are glycosyltransferases	474:503	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	0	55	theme	ALG6	60:63	arg1	mechanism					14:22	mechanism	14:22	mechanism	14:22	Structure and mechanism of the ER-based glucosyltransferase ALG6.					
32103179	0	55	theme	ALG6	60:63	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and mechanism of the ER-based glucosyltransferase ALG6.					
32103179	5	56	theme	variable	956:963	arg1	module					965:970	a variable module	954:970	a variable module	954:970	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	6	57	theme	donor	1149:1153	arg1	substrates					1168:1177	donor and acceptor substrates	1149:1177	donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro	1149:1265	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32103179	2	58	theme	final	263:267	arg1	steps					275:279	The final seven steps	259:279	The final seven steps	259:279	The final seven steps occur in the lumen of the endoplasmic reticulum (ER) and require dolichylphosphate-activated mannose and glucose as donor substrates2.					
32103179	8	59	theme	aspartate	1490:1498	arg1	residue					1500:1506	a catalytic aspartate residue	1478:1506	a catalytic aspartate residue that probably acts as a general base	1478:1543	Functional analysis of ALG6 variants identified a catalytic aspartate residue that probably acts as a general base.					
32103179	8	59	theme	aspartate	1490:1498	arg1	base					1540:1543	a general base	1530:1543	a general base	1530:1543	Functional analysis of ALG6 variants identified a catalytic aspartate residue that probably acts as a general base.					
32103179	7	60	theme	active	1403:1408	arg1	enzyme					1422:1427	the enzyme	1418:1427	the enzyme	1418:1427	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution revealed the active site of the enzyme.					
32103179	7	60	theme	active	1403:1408	arg1	site					1410:1413	the active site	1399:1413	the active site of the enzyme	1399:1427	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution revealed the active site of the enzyme.					
32103179	0	61	theme	glucosyltransferase	40:58	arg1	ALG6					60:63	the ER-based glucosyltransferase ALG6	27:63	the ER-based glucosyltransferase ALG6	27:63	Structure and mechanism of the ER-based glucosyltransferase ALG6.					
32103179	10	62	theme	GT-C	1647:1650	arg1	enzymes					1652:1658	ER-luminal GT-C enzymes	1636:1658	ER-luminal GT-C enzymes	1636:1658	Our results define the architecture of ER-luminal GT-C enzymes and provide a structural basis for understanding their catalytic mechanisms.					
32103179	3	63	theme	isoprenoid-linked	619:635	arg1	substrate3,4					656:667	an isoprenoid-linked carbohydrate donor substrate3,4	616:667	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.	416:668	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	1	64	theme	glycosyltransferases	117:136	arg1	series					107:112	a series	105:112	a series of glycosyltransferases	105:136	In eukaryotic protein N-glycosylation, a series of glycosyltransferases catalyse the biosynthesis of a dolichylpyrophosphate-linked oligosaccharide before its transfer onto acceptor proteins1.					
32103179	10	65	theme	ER-luminal	1636:1645	arg1	enzymes					1652:1658	ER-luminal GT-C enzymes	1636:1658	ER-luminal GT-C enzymes	1636:1658	Our results define the architecture of ER-luminal GT-C enzymes and provide a structural basis for understanding their catalytic mechanisms.					
32103179	2	66	theme	dolichylphosphate-activated	346:372	arg1	mannose					374:380	dolichylphosphate-activated mannose	346:380	dolichylphosphate-activated mannose	346:380	The final seven steps occur in the lumen of the endoplasmic reticulum (ER) and require dolichylphosphate-activated mannose and glucose as donor substrates2.					
32103179	3	67	theme	C-superfamily	512:524	arg1	enzymes-ALG3					432:443	The responsible enzymes-ALG3	416:443	The responsible enzymes-ALG3	416:443	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	3	67	theme	C-superfamily	512:524	arg1	ALG6					459:462	ALG6	459:462	ALG6	459:462	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	3	67	theme	C-superfamily	512:524	arg1	ALG8					465:468	ALG8	465:468	ALG8	465:468	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	3	67	theme	C-superfamily	512:524	arg1	processing					605:614	processing	605:614	processing	605:614	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	3	67	theme	C-superfamily	512:524	arg1	ALG9					446:449	ALG9	446:449	ALG9	446:449	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	3	67	theme	C-superfamily	512:524	arg1	ALG12					452:456	ALG12	452:456	ALG12	452:456	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	3	67	theme	C-superfamily	512:524	arg1	glycosyltransferases					484:503	ALG10-are glycosyltransferases	474:503	ALG10-are glycosyltransferases	474:503	The responsible enzymes-ALG3, ALG9, ALG12, ALG6, ALG8 and ALG10-are glycosyltransferases of the C-superfamily (GT-Cs), which are loosely defined as containing membrane-spanning helices and processing an isoprenoid-linked carbohydrate donor substrate3,4.					
32103179	8	68	theme	ALG6	1453:1456	arg1	variants					1458:1465	ALG6 variants	1453:1465	ALG6 variants	1453:1465	Functional analysis of ALG6 variants identified a catalytic aspartate residue that probably acts as a general base.					
32103179	5	69	theme	functional	992:1001	arg1	roles					1003:1007	distinct functional roles	983:1007	distinct functional roles	983:1007	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	10	70	theme	structural	1674:1683	arg1	basis					1685:1689	a structural basis	1672:1689	a structural basis for understanding their catalytic mechanisms	1672:1734	Our results define the architecture of ER-luminal GT-C enzymes and provide a structural basis for understanding their catalytic mechanisms.					
32103179	4	71	theme	cryo-electron	690:702	arg1	microscopy					704:713	cryo-electron microscopy	690:713	the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold	686:824	Here we present the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold.					
32103179	7	72	theme	enzyme	1422:1427	arg1	enzyme					1422:1427	the enzyme	1418:1427	the enzyme	1418:1427	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution revealed the active site of the enzyme.					
32103179	7	72	theme	enzyme	1422:1427	arg1	site					1410:1413	the active site	1399:1413	the active site of the enzyme	1399:1427	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution revealed the active site of the enzyme.					
32103179	4	73	theme	3.0 Å	742:746	arg1	resolution					748:757	3.0 Å resolution	742:757	3.0 Å resolution	742:757	Here we present the cryo-electron microscopy structure of yeast ALG6 at 3.0 Å resolution, which reveals a previously undescribed transmembrane protein fold.					
32103179	5	74	with	Comparison	827:836	arg1	structures					857:866	reported GT-C structures	843:866	reported GT-C structures	843:866	Comparison with reported GT-C structures suggests that GT-C enzymes contain a modular architecture with a conserved module and a variable module, each with distinct functional roles.					
32103179	10	75	theme	catalytic	1715:1723	arg1	mechanisms					1725:1734	their catalytic mechanisms	1709:1734	their catalytic mechanisms	1709:1734	Our results define the architecture of ER-luminal GT-C enzymes and provide a structural basis for understanding their catalytic mechanisms.					
32103179	2	76	theme	donor	397:401	arg1	substrates2					403:413	donor substrates2	397:413	donor substrates2	397:413	The final seven steps occur in the lumen of the endoplasmic reticulum (ER) and require dolichylphosphate-activated mannose and glucose as donor substrates2.					
32103179	7	77	theme	dolichylphosphate-glucose	1344:1368	arg1	analogue					1332:1339	an analogue	1329:1339	an analogue of dolichylphosphate-glucose	1329:1368	A second cryo-electron microscopy structure of ALG6 bound to an analogue of dolichylphosphate-glucose at 3.9 Å resolution revealed the active site of the enzyme.					
32103179	6	78	theme	glycan	1120:1125	arg1	extension					1127:1135	enzymatic glycan extension	1110:1135	enzymatic glycan extension	1110:1135	We used synthetic analogues of dolichylphosphate-linked and dolichylpyrophosphate-linked sugars and enzymatic glycan extension to generate donor and acceptor substrates using purified enzymes of the ALG pathway to recapitulate the activity of ALG6 in vitro.					
32702186	8	0	theme	feces	1104:1108	arg1	transfer					1110:1117	horizontal feces transfer	1093:1117	horizontal feces transfer from Aap-treated mice to normal mice	1093:1154	Furthermore, the preventing effect was transmissible via horizontal feces transfer from Aap-treated mice to normal mice.					
32702186	6	1	theme	colon	771:775	arg1	damage					777:782	colon damage	771:782	colon damage	771:782	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	2	2	theme	changing	341:348	arg1	habits					357:362	the changing eating habits	337:362	the changing eating habits	337:362	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	0	3	attach	isolated	15:22	arg2	Polysaccharide					0:13	Polysaccharide	0:13	Polysaccharide isolated from Auricularia auricular-judae (Bull.)	0:63	Polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	0	3	attach	isolated	15:22	arg2	Bull					58:61	Bull	58:61	Bull	58:61	Polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	0	3	attach	isolated	15:22	arg1	auricular-judae					41:55	Auricularia auricular-judae	29:55	Auricularia auricular-judae	29:55	Polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	11	4	theme	foods	1517:1521	arg1	development					1483:1493	the development	1479:1493	the development of related functional foods	1479:1521	This research might lay the theoretical foundation and technical support for the development of related functional foods.					
32702186	8	5	theme	normal	1144:1149	arg1	mice					1151:1154	normal mice	1144:1154	normal mice	1144:1154	Furthermore, the preventing effect was transmissible via horizontal feces transfer from Aap-treated mice to normal mice.					
32702186	3	6	attach	isolated	432:439	arg1	auricular-judae					458:472	Auricularia auricular-judae	446:472	Auricularia auricular-judae	446:472	In previous research, we found that a water-soluble polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	3	6	attach	isolated	432:439	arg2	polysaccharide					417:430	a water-soluble polysaccharide	401:430	a water-soluble polysaccharide isolated from Auricularia auricular-judae	401:472	In previous research, we found that a water-soluble polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	6	7	from	inflammation	797:808	arg1	mice					821:824	colitis mice	813:824	colitis mice	813:824	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	10	8	theme	DSS-induced	1373:1383	arg1	colitis					1385:1391	DSS-induced colitis	1373:1391	DSS-induced colitis in mice	1373:1399	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.					
32702186	7	9	theme	microbiota	1012:1021	arg1	composition					1023:1033	gut microbiota composition	1008:1033	gut microbiota composition	1008:1033	Through high-throughput sequencing, we found that Aap changed gut microbiota composition.					
32702186	9	10	theme	oral	1184:1187	arg1	substance					1237:1245	a promising protective substance	1214:1245	a promising protective substance of IBD	1214:1252	Our results indicated that oral administration of Aap is a promising protective substance of IBD.					
32702186	9	10	theme	oral	1184:1187	arg1	administration					1189:1202	oral administration	1184:1202	oral administration of Aap	1184:1209	Our results indicated that oral administration of Aap is a promising protective substance of IBD.					
32702186	6	11	theme	D-lactic	895:902	arg1	acid					904:907	the D-lactic acid and diamine oxidase level	891:933	acid	904:907	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	6	12	from	damage	777:782	arg1	mice					821:824	colitis mice	813:824	colitis mice	813:824	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	2	13	theme	intestinal	225:234	arg1	disease					236:242	a chronic intestinal disease	215:242	a chronic intestinal disease	215:242	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	2	13	theme	intestinal	225:234	arg1	disease					198:204	Inflammatory bowel disease	179:204	Inflammatory bowel disease (IBD)	179:210	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	7	14	theme	high-throughput	954:968	arg1	sequencing					970:979	high-throughput sequencing	954:979	high-throughput sequencing	954:979	Through high-throughput sequencing, we found that Aap changed gut microbiota composition.					
32702186	6	15	theme	barrier	871:877	arg1	damage					850:855	the damage	846:855	the damage of intestinal barrier	846:877	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	2	16	from	Asia	321:324	arg1	prevalent					308:316	prevalent	308:316	prevalent	308:316	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	9	17	theme	promising	1216:1224	arg1	substance					1237:1245	a promising protective substance	1214:1245	a promising protective substance of IBD	1214:1252	Our results indicated that oral administration of Aap is a promising protective substance of IBD.					
32702186	9	17	theme	promising	1216:1224	arg1	administration					1189:1202	oral administration	1184:1202	oral administration of Aap	1184:1209	Our results indicated that oral administration of Aap is a promising protective substance of IBD.					
32702186	13	18	theme	broad	1699:1703	arg1	significance					1705:1716	the broad significance	1695:1716	the broad significance in nutrition and food function	1695:1747	Auricularia auricular as a highly nutritious agricultural product showed the broad significance in nutrition and food function.					
32702186	11	19	theme	functional	1506:1515	arg1	foods					1517:1521	related functional foods	1498:1521	related functional foods	1498:1521	This research might lay the theoretical foundation and technical support for the development of related functional foods.					
32702186	9	20	theme	IBD	1250:1252	arg1	substance					1237:1245	a promising protective substance	1214:1245	a promising protective substance of IBD	1214:1252	Our results indicated that oral administration of Aap is a promising protective substance of IBD.					
32702186	9	20	theme	IBD	1250:1252	arg1	administration					1189:1202	oral administration	1184:1202	oral administration of Aap	1184:1209	Our results indicated that oral administration of Aap is a promising protective substance of IBD.					
32702186	1	21	theme	microbiota	167:176	arg1	composition					144:154	the composition	140:154	the composition of the gut microbiota	140:176	prevents dextran sulfate sodium-induced colitis in mice through modulating the composition of the gut microbiota.					
32702186	4	22	theme	mushroom	499:506	arg1	-is					513:515	edible mushroom (Aap)-is	492:515	edible mushroom (Aap)-is	492:515	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	6	23	theme	oxidase	921:927	arg1	level					929:933	the D-lactic acid and diamine oxidase level	891:933	level	929:933	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	6	24	from	level	929:933	arg1	plasma					938:943	plasma	938:943	plasma	938:943	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	1	25	theme	sulfate	82:88	arg1	colitis					105:111	dextran sulfate sodium-induced colitis	74:111	dextran sulfate sodium-induced colitis in mice	74:119	prevents dextran sulfate sodium-induced colitis in mice through modulating the composition of the gut microbiota.					
32702186	3	26	theme	water-soluble	403:415	arg1	polysaccharide					417:430	a water-soluble polysaccharide	401:430	a water-soluble polysaccharide isolated from Auricularia auricular-judae	401:472	In previous research, we found that a water-soluble polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	6	27	from	acid	904:907	arg1	plasma					938:943	plasma	938:943	plasma	938:943	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	6	28	theme	weight	758:763	arg1	loss					765:768	weight loss	758:768	weight loss	758:768	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	11	29	theme	theoretical	1430:1440	arg1	foundation					1442:1451	theoretical foundation	1430:1451	theoretical foundation	1430:1451	This research might lay the theoretical foundation and technical support for the development of related functional foods.					
32702186	4	30	theme	glycosidic	535:544	arg1	substance					600:608	therapeutic or protective substance	574:608	therapeutic or protective substance in enteritis	574:621	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	4	30	theme	glycosidic	535:544	arg1	bonds					546:550	β-1,3 glycosidic bonds	529:550	β-1,3 glycosidic bonds	529:550	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	2	31	theme	bowel	192:196	arg1	IBD					207:209	IBD	207:209	IBD	207:209	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	2	31	theme	bowel	192:196	arg1	disease					198:204	Inflammatory bowel disease	179:204	Inflammatory bowel disease (IBD)	179:210	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	2	31	theme	bowel	192:196	arg1	disease					236:242	a chronic intestinal disease	215:242	a chronic intestinal disease	215:242	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	10	32	theme	PRACTICAL	1255:1263	arg1	APPLICATION					1265:1275	PRACTICAL APPLICATION	1255:1275	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.	1255:1400	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.					
32702186	3	33	theme	Auricularia	446:456	arg1	auricular-judae					458:472	Auricularia auricular-judae	446:472	Auricularia auricular-judae	446:472	In previous research, we found that a water-soluble polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	12	34	from	auricular	1591:1599	arg1	life					1616:1619	their daily life	1604:1619	their daily life	1604:1619	People could also enhance their gut immunity by eating Auricularia auricular in their daily life.					
32702186	13	35	theme	nutritious	1656:1665	arg1	product					1680:1686	a highly nutritious agricultural product	1647:1686	a highly nutritious agricultural product	1647:1686	Auricularia auricular as a highly nutritious agricultural product showed the broad significance in nutrition and food function.					
32702186	10	36	contain	had	1336:1338	arg1	polysaccharide					1321:1334	Auricularia auricula polysaccharide	1300:1334	Auricularia auricula polysaccharide	1300:1334	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.					
32702186	10	36	contain	had	1336:1338	arg2	effect					1363:1368	substantial preventing effect	1340:1368	substantial preventing effect	1340:1368	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.					
32702186	8	37	from	mice	1136:1139	arg1	transfer					1110:1117	horizontal feces transfer	1093:1117	horizontal feces transfer from Aap-treated mice to normal mice	1093:1154	Furthermore, the preventing effect was transmissible via horizontal feces transfer from Aap-treated mice to normal mice.					
32702186	4	38	theme	-is	513:515	arg1	kind					484:487	-a kind	481:487	-a kind of edible mushroom (Aap)-is	481:515	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	13	39	theme	Auricularia	1622:1632	arg1	auricular					1634:1642	Auricularia auricular	1622:1642	Auricularia auricular as a highly nutritious agricultural product	1622:1686	Auricularia auricular as a highly nutritious agricultural product showed the broad significance in nutrition and food function.					
32702186	1	40	from	colitis	105:111	arg1	mice					116:119	mice	116:119	mice	116:119	prevents dextran sulfate sodium-induced colitis in mice through modulating the composition of the gut microbiota.					
32702186	6	41	theme	intestinal	860:869	arg1	barrier					871:877	intestinal barrier	860:877	intestinal barrier	860:877	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	10	42	from	colitis	1385:1391	arg1	mice					1396:1399	mice	1396:1399	mice	1396:1399	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.					
32702186	10	43	theme	auricula	1312:1319	arg1	polysaccharide					1321:1334	Auricularia auricula polysaccharide	1300:1334	Auricularia auricula polysaccharide	1300:1334	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.					
32702186	2	44	theme	eating	350:355	arg1	habits					357:362	the changing eating habits	337:362	the changing eating habits	337:362	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	8	45	theme	horizontal	1093:1102	arg1	transfer					1110:1117	horizontal feces transfer	1093:1117	horizontal feces transfer from Aap-treated mice to normal mice	1093:1154	Furthermore, the preventing effect was transmissible via horizontal feces transfer from Aap-treated mice to normal mice.					
32702186	10	46	theme	preventing	1352:1361	arg1	effect					1363:1368	substantial preventing effect	1340:1368	substantial preventing effect	1340:1368	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.					
32702186	8	47	theme	Aap-treated	1124:1134	arg1	mice					1136:1139	Aap-treated mice	1124:1139	Aap-treated mice	1124:1139	Furthermore, the preventing effect was transmissible via horizontal feces transfer from Aap-treated mice to normal mice.					
32702186	6	48	theme	diamine	913:919	arg1	level					929:933	the D-lactic acid and diamine oxidase level	891:933	level	929:933	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	6	49	theme	mucosal	789:795	arg1	inflammation					797:808	mucosal inflammation	789:808	mucosal inflammation in colitis mice	789:824	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	7	50	theme	gut	1008:1010	arg1	composition					1023:1033	gut microbiota composition	1008:1033	gut microbiota composition	1008:1033	Through high-throughput sequencing, we found that Aap changed gut microbiota composition.					
32702186	6	51	theme	Aap	733:735	arg1	pre-administration					711:728	pre-administration	711:728	pre-administration of Aap	711:735	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	12	52	theme	daily	1610:1614	arg1	life					1616:1619	their daily life	1604:1619	their daily life	1604:1619	People could also enhance their gut immunity by eating Auricularia auricular in their daily life.					
32702186	4	53	from	substance	600:608	arg1	enteritis					613:621	enteritis	613:621	enteritis	613:621	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	9	54	theme	Aap	1207:1209	arg1	substance					1237:1245	a promising protective substance	1214:1245	a promising protective substance of IBD	1214:1252	Our results indicated that oral administration of Aap is a promising protective substance of IBD.					
32702186	9	54	theme	Aap	1207:1209	arg1	administration					1189:1202	oral administration	1184:1202	oral administration of Aap	1184:1209	Our results indicated that oral administration of Aap is a promising protective substance of IBD.					
32702186	2	55	theme	chronic	217:223	arg1	disease					236:242	a chronic intestinal disease	215:242	a chronic intestinal disease	215:242	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	2	55	theme	chronic	217:223	arg1	disease					198:204	Inflammatory bowel disease	179:204	Inflammatory bowel disease (IBD)	179:210	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	12	56	theme	Auricularia	1579:1589	arg1	auricular					1591:1599	Auricularia auricular	1579:1599	Auricularia auricular in their daily life	1579:1619	People could also enhance their gut immunity by eating Auricularia auricular in their daily life.					
32702186	5	57	theme	preventing	655:664	arg1	effect					666:671	the preventing effect	651:671	the preventing effect of Aap on IBD	651:685	We therefore aimed to find the preventing effect of Aap on IBD.					
32702186	11	58	theme	technical	1457:1465	arg1	support					1467:1473	technical support	1457:1473	technical support	1457:1473	This research might lay the theoretical foundation and technical support for the development of related functional foods.					
32702186	9	59	theme	protective	1226:1235	arg1	substance					1237:1245	a promising protective substance	1214:1245	a promising protective substance of IBD	1214:1252	Our results indicated that oral administration of Aap is a promising protective substance of IBD.					
32702186	9	59	theme	protective	1226:1235	arg1	administration					1189:1202	oral administration	1184:1202	oral administration of Aap	1184:1209	Our results indicated that oral administration of Aap is a promising protective substance of IBD.					
32702186	12	60	theme	gut	1556:1558	arg1	immunity					1560:1567	their gut immunity	1550:1567	their gut immunity	1550:1567	People could also enhance their gut immunity by eating Auricularia auricular in their daily life.					
32702186	4	61	theme	edible	492:497	arg1	Aap					509:511	Aap	509:511	Aap	509:511	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	4	61	theme	edible	492:497	arg1	mushroom					499:506	edible mushroom	492:506	edible mushroom (Aap)-is	492:515	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	11	62	dep	foundation	1442:1451	arg1	the					1426:1428	the	1426:1428	the	1426:1428	This research might lay the theoretical foundation and technical support for the development of related functional foods.					
32702186	11	63	theme	related	1498:1504	arg1	foods					1517:1521	related functional foods	1498:1521	related functional foods	1498:1521	This research might lay the theoretical foundation and technical support for the development of related functional foods.					
32702186	1	64	theme	gut	163:165	arg1	microbiota					167:176	the gut microbiota	159:176	the gut microbiota	159:176	prevents dextran sulfate sodium-induced colitis in mice through modulating the composition of the gut microbiota.					
32702186	5	65	theme	Aap	676:678	arg1	effect					666:671	the preventing effect	651:671	the preventing effect of Aap on IBD	651:685	We therefore aimed to find the preventing effect of Aap on IBD.					
32702186	2	66	from	prevalent	308:316	arg1	Asia					321:324	Asia	321:324	Asia	321:324	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	1	67	theme	dextran	74:80	arg1	sulfate					82:88	dextran sulfate	74:88	dextran sulfate sodium-induced colitis in mice	74:119	prevents dextran sulfate sodium-induced colitis in mice through modulating the composition of the gut microbiota.					
32702186	6	68	from	loss	765:768	arg1	mice					821:824	colitis mice	813:824	colitis mice	813:824	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	3	69	dep	found	390:394	arg1	Bull					475:478	Bull	475:478	Bull	475:478	In previous research, we found that a water-soluble polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	1	70	theme	sodium-induced	90:103	arg1	colitis					105:111	dextran sulfate sodium-induced colitis	74:111	dextran sulfate sodium-induced colitis in mice	74:119	prevents dextran sulfate sodium-induced colitis in mice through modulating the composition of the gut microbiota.					
32702186	4	71	theme	β-1,3	529:533	arg1	substance					600:608	therapeutic or protective substance	574:608	therapeutic or protective substance in enteritis	574:621	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	4	71	theme	β-1,3	529:533	arg1	bonds					546:550	β-1,3 glycosidic bonds	529:550	β-1,3 glycosidic bonds	529:550	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	13	72	theme	agricultural	1667:1678	arg1	product					1680:1686	a highly nutritious agricultural product	1647:1686	a highly nutritious agricultural product	1647:1686	Auricularia auricular as a highly nutritious agricultural product showed the broad significance in nutrition and food function.					
32702186	0	73	theme	Auricularia	29:39	arg1	auricular-judae					41:55	Auricularia auricular-judae	29:55	Auricularia auricular-judae	29:55	Polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	3	74	theme	previous	368:375	arg1	research					377:384	previous research	368:384	previous research	368:384	In previous research, we found that a water-soluble polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	6	75	theme	colitis	813:819	arg1	mice					821:824	colitis mice	813:824	colitis mice	813:824	Here, we reported that pre-administration of Aap not only ameliorated weight loss, colon damage, and mucosal inflammation in colitis mice, but also prevented the damage of intestinal barrier by reducing the D-lactic acid and diamine oxidase level in plasma.					
32702186	13	76	theme	food	1735:1738	arg1	function					1740:1747	food function	1735:1747	food function	1735:1747	Auricularia auricular as a highly nutritious agricultural product showed the broad significance in nutrition and food function.					
32702186	2	77	theme	Inflammatory	179:190	arg1	IBD					207:209	IBD	207:209	IBD	207:209	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	2	77	theme	Inflammatory	179:190	arg1	disease					198:204	Inflammatory bowel disease	179:204	Inflammatory bowel disease (IBD)	179:210	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	2	77	theme	Inflammatory	179:190	arg1	disease					236:242	a chronic intestinal disease	215:242	a chronic intestinal disease	215:242	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	10	78	theme	Auricularia	1300:1310	arg1	polysaccharide					1321:1334	Auricularia auricula polysaccharide	1300:1334	Auricularia auricula polysaccharide	1300:1334	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.					
32702186	8	79	theme	preventing	1053:1062	arg1	effect					1064:1069	the preventing effect	1049:1069	the preventing effect	1049:1069	Furthermore, the preventing effect was transmissible via horizontal feces transfer from Aap-treated mice to normal mice.					
32702186	2	80	located	found	264:268	arg1	westerners					273:282	westerners	273:282	westerners	273:282	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	2	80	located	found	264:268	arg2	disease					198:204	Inflammatory bowel disease	179:204	Inflammatory bowel disease (IBD)	179:210	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	2	80	located	found	264:268	arg2	disease					236:242	a chronic intestinal disease	215:242	a chronic intestinal disease	215:242	Inflammatory bowel disease (IBD) is a chronic intestinal disease, which was commonly found in westerners whereas is increasingly prevalent in Asia because of the changing eating habits.					
32702186	13	81	from	significance	1705:1716	arg1	function					1740:1747	food function	1735:1747	food function	1735:1747	Auricularia auricular as a highly nutritious agricultural product showed the broad significance in nutrition and food function.					
32702186	13	81	from	significance	1705:1716	arg1	nutrition					1721:1729	nutrition	1721:1729	nutrition	1721:1729	Auricularia auricular as a highly nutritious agricultural product showed the broad significance in nutrition and food function.					
32702186	4	82	theme	therapeutic	574:584	arg1	substance					600:608	therapeutic or protective substance	574:608	therapeutic or protective substance in enteritis	574:621	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	4	82	theme	therapeutic	574:584	arg1	bonds					546:550	β-1,3 glycosidic bonds	529:550	β-1,3 glycosidic bonds	529:550	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	10	83	dep	APPLICATION	1265:1275	arg1	proved					1288:1293	proved	1288:1293	proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice	1288:1399	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.					
32702186	10	84	theme	substantial	1340:1350	arg1	effect					1363:1368	substantial preventing effect	1340:1368	substantial preventing effect	1340:1368	PRACTICAL APPLICATION: Our study proved that Auricularia auricula polysaccharide had substantial preventing effect on DSS-induced colitis in mice.					
32702186	5	85	from	effect	666:671	arg1	IBD					683:685	IBD	683:685	IBD	683:685	We therefore aimed to find the preventing effect of Aap on IBD.					
32702186	0	86	dep	isolated	15:22	arg1	Polysaccharide					0:13	Polysaccharide	0:13	Polysaccharide isolated from Auricularia auricular-judae (Bull.)	0:63	Polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	0	86	dep	isolated	15:22	arg1	Bull					58:61	Bull	58:61	Bull	58:61	Polysaccharide isolated from Auricularia auricular-judae (Bull.)					
32702186	4	87	theme	protective	589:598	arg1	substance					600:608	therapeutic or protective substance	574:608	therapeutic or protective substance in enteritis	574:621	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
32702186	4	87	theme	protective	589:598	arg1	bonds					546:550	β-1,3 glycosidic bonds	529:550	β-1,3 glycosidic bonds	529:550	-a kind of edible mushroom (Aap)-is composed of β-1,3 glycosidic bonds, which is regarded as therapeutic or protective substance in enteritis.					
33454012	8	0	from	differences	1673:1683	arg1	patterns					1713:1720	oligosaccharide cleavage patterns	1688:1720	oligosaccharide cleavage patterns	1688:1720	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	2	1	theme	new	375:377	arg1	GHs					379:381	new GHs	375:381	new GHs	375:381	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	1	2	theme	polysaccharides	266:280	arg1	range					257:261	a diverse range	247:261	a diverse range of polysaccharides	247:280	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	6	3	theme	mechanistic	971:981	arg1	understanding					983:995	A mechanistic understanding	969:995	A mechanistic understanding of GH5_4 substrate specificity	969:1026	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	10	4	theme	GH5_4	1909:1913	arg1	endoglucanases					1915:1928	GH5_4 endoglucanases	1909:1928	GH5_4 endoglucanases	1909:1928	We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.					
33454012	7	5	theme	GH5_4	1202:1206	arg1	enzymes					1208:1214	10 new GH5_4 enzymes	1195:1214	10 new GH5_4 enzymes from cellulolytic microbes	1195:1241	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	5	6	theme	new	860:862	arg1	endoglucanases					882:895	100 new broad-specificity endoglucanases	856:895	100 new broad-specificity endoglucanases	856:895	We previously surveyed the whole subfamily and found over 100 new broad-specificity endoglucanases, although the structural origins of broad specificity remained unclear.					
33454012	4	7	theme	broad-selectivity	700:716	arg1	endoglucanases					718:731	numerous broad-selectivity endoglucanases	691:731	numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans	691:795	Among these, subfamily 4 (GH5_4) contains numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans.					
33454012	10	8	theme	broad	1939:1943	arg1	specificity					1945:1955	broad specificity	1939:1955	broad specificity	1939:1955	We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.					
33454012	5	9	theme	specificity	939:949	arg1	origins					922:928	the structural origins	907:928	the structural origins of broad specificity	907:949	We previously surveyed the whole subfamily and found over 100 new broad-specificity endoglucanases, although the structural origins of broad specificity remained unclear.					
33454012	6	10	theme	best	1050:1053	arg1	design					1063:1068	the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases	1046:1163	design	1063:1068	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	1	11	theme	biomass	215:221	arg1	hydrolysis					223:232	plant biomass hydrolysis	209:232	plant biomass hydrolysis	209:232	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	8	12	theme	key	1556:1558	arg1	residues					1569:1576	key aromatic residues	1556:1576	key aromatic residues	1556:1576	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	5	13	theme	broad-specificity	864:880	arg1	endoglucanases					882:895	100 new broad-specificity endoglucanases	856:895	100 new broad-specificity endoglucanases	856:895	We previously surveyed the whole subfamily and found over 100 new broad-specificity endoglucanases, although the structural origins of broad specificity remained unclear.					
33454012	5	14	theme	structural	911:920	arg1	origins					922:928	the structural origins	907:928	the structural origins of broad specificity	907:949	We previously surveyed the whole subfamily and found over 100 new broad-specificity endoglucanases, although the structural origins of broad specificity remained unclear.					
33454012	9	15	theme	industrial	1869:1878	arg1	enzymes					1880:1886	industrial enzymes	1869:1886	industrial enzymes	1869:1886	Our new composite model identifies several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes.					
33454012	8	16	theme	residues	1569:1576	arg1	positions					1543:1551	The positions	1539:1551	The positions of key aromatic residues	1539:1576	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	6	17	theme	substrate	1006:1014	arg1	specificity					1016:1026	GH5_4 substrate specificity	1000:1026	GH5_4 substrate specificity	1000:1026	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	7	18	theme	opportunistic	1472:1484	arg1	reactions					1496:1504	opportunistic secondary reactions	1472:1504	opportunistic secondary reactions on cellulose, mannan, and xylan	1472:1536	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	6	19	theme	industrial	1106:1115	arg1	application					1117:1127	the most appropriate industrial application	1085:1127	the most appropriate industrial application of broad-specificity endoglucanases	1085:1163	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	8	20	theme	reaction	1600:1607	arg1	rate					1609:1612	the overall reaction rate	1588:1612	the overall reaction rate	1588:1612	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	7	21	theme	GH5_4	1349:1353	arg1	enzymes					1355:1361	GH5_4 enzymes	1349:1361	GH5_4 enzymes	1349:1361	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	22	contain	have	1363:1366	arg1	enzymes					1355:1361	GH5_4 enzymes	1349:1361	GH5_4 enzymes	1349:1361	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	22	contain	have	1363:1366	arg2	efficiency					1390:1399	the highest catalytic efficiency	1368:1399	the highest catalytic efficiency	1368:1399	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	1	23	theme	glycoside	168:176	arg1	GHs					190:192	GHs	190:192	GHs	190:192	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	1	23	theme	glycoside	168:176	arg1	hydrolases					178:187	Broad-specificity glycoside hydrolases	150:187	Broad-specificity glycoside hydrolases (GHs)	150:193	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	6	24	dep	design	1063:1068	arg1	strategies					1070:1079	strategies	1070:1079	strategies	1070:1079	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	7	25	dep	selectivity	1276:1286	arg1	using					1288:1292	using	1288:1292	using normalized reducing sugar assays	1288:1325	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	25	dep	selectivity	1276:1286	arg1	MS.					1331:1333	MS.	1331:1333	MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan	1331:1536	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	5	26	theme	whole	825:829	arg1	subfamily					831:839	the whole subfamily	821:839	the whole subfamily	821:839	We previously surveyed the whole subfamily and found over 100 new broad-specificity endoglucanases, although the structural origins of broad specificity remained unclear.					
33454012	0	27	theme	substrate	86:94	arg1	specificity					96:106	broad substrate specificity	80:106	broad substrate specificity	80:106	A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.					
33454012	8	28	theme	tolerance	1639:1647	arg1	breadth					1618:1624	breadth	1618:1624	breadth	1618:1624	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	8	28	theme	tolerance	1639:1647	arg1	rate					1609:1612	the overall reaction rate	1588:1612	the overall reaction rate	1588:1612	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	6	29	theme	endoglucanases	1150:1163	arg1	design					1063:1068	the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases	1046:1163	design	1063:1068	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	6	29	theme	endoglucanases	1150:1163	arg1	application					1117:1127	the most appropriate industrial application	1085:1127	the most appropriate industrial application of broad-specificity endoglucanases	1085:1163	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	7	30	theme	β-glucans	1456:1464	arg1	hydrolysis					1405:1414	hydrolysis	1405:1414	hydrolysis of xyloglucan, glucomannan, and soluble β-glucans	1405:1464	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	31	theme	reducing	1305:1312	arg1	assays					1320:1325	normalized reducing sugar assays	1294:1325	normalized reducing sugar assays	1294:1325	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	32	from	reactions	1496:1504	arg1	mannan					1520:1525	mannan	1520:1525	mannan	1520:1525	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	32	from	reactions	1496:1504	arg1	xylan					1532:1536	xylan	1532:1536	xylan	1532:1536	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	32	from	reactions	1496:1504	arg1	cellulose					1509:1517	cellulose	1509:1517	cellulose	1509:1517	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	33	theme	glucomannan	1431:1441	arg1	β-glucans					1456:1464	xyloglucan, glucomannan, and soluble β-glucans	1419:1464	xyloglucan, glucomannan, and soluble β-glucans	1419:1464	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	3	34	link	-linked	613:619	arg1	oligo-					621:626	oligo-	621:626	oligo-	621:626	GH5 has over 50 subfamilies exhibiting selectivities for reaction with β-(1,4)-linked oligo- and polysaccharides.					
33454012	2	35	theme	substrate-binding	433:449	arg1	sites					451:455	more tolerant substrate-binding sites	419:455	more tolerant substrate-binding sites	419:455	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	7	36	theme	substrate	1266:1274	arg1	selectivity					1276:1286	their substrate selectivity	1260:1286	their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan	1260:1536	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	1	37	theme	renewable	316:324	arg1	energy					326:331	renewable energy	316:331	renewable energy	316:331	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	9	38	theme	new	1727:1729	arg1	model					1741:1745	Our new composite model	1723:1745	Our new composite model	1723:1745	Our new composite model identifies several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes.					
33454012	7	39	theme	xyloglucan	1419:1428	arg1	β-glucans					1456:1464	xyloglucan, glucomannan, and soluble β-glucans	1419:1464	xyloglucan, glucomannan, and soluble β-glucans	1419:1464	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	2	40	theme	bioenergy	500:508	arg1	production					510:519	renewable bioenergy production	490:519	renewable bioenergy production	490:519	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	8	41	theme	oligosaccharide	1688:1702	arg1	patterns					1713:1720	oligosaccharide cleavage patterns	1688:1720	oligosaccharide cleavage patterns	1688:1720	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	10	42	theme	valuable	2006:2013	arg1	addition					2015:2022	a valuable addition	2004:2022	a valuable addition to the biomass deconstruction toolset	2004:2060	We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.					
33454012	0	43	theme	GH5_4	35:39	arg1	endoglucanases					41:54	GH5_4 endoglucanases	35:54	GH5_4 endoglucanases	35:54	A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.					
33454012	7	44	from	microbes	1234:1241	arg1	enzymes					1208:1214	10 new GH5_4 enzymes	1195:1214	10 new GH5_4 enzymes from cellulolytic microbes	1195:1241	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	44	from	microbes	1234:1241	arg1	structures					1181:1190	structures	1181:1190	structures of 10 new GH5_4 enzymes from cellulolytic microbes	1181:1241	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	9	45	theme	several	1758:1764	arg1	features					1786:1793	several critical structural features	1758:1793	several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes	1758:1886	Our new composite model identifies several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes.					
33454012	4	46	contain	contains	682:689	arg2	endoglucanases					718:731	numerous broad-selectivity endoglucanases	691:731	numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans	691:795	Among these, subfamily 4 (GH5_4) contains numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans.					
33454012	4	46	contain	contains	682:689	arg1	subfamily					662:670	subfamily 4	662:672	subfamily 4 (GH5_4)	662:680	Among these, subfamily 4 (GH5_4) contains numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans.					
33454012	4	46	contain	contains	682:689	arg1	GH5_4					675:679	GH5_4	675:679	GH5_4	675:679	Among these, subfamily 4 (GH5_4) contains numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans.					
33454012	2	47	theme	improved	388:395	arg1	parameters					405:414	improved kinetic parameters	388:414	improved kinetic parameters	388:414	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	1	48	theme	diverse	249:255	arg1	range					257:261	a diverse range	247:261	a diverse range of polysaccharides	247:280	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	0	49	theme	endoglucanases	41:54	arg1	survey					25:30	A structural and kinetic survey	0:30	A structural and kinetic survey of GH5_4 endoglucanases	0:54	A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.					
33454012	6	50	theme	GH5_4	1000:1004	arg1	specificity					1016:1026	GH5_4 substrate specificity	1000:1026	GH5_4 substrate specificity	1000:1026	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	9	51	theme	structural	1775:1784	arg1	features					1786:1793	several critical structural features	1758:1793	several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes	1758:1886	Our new composite model identifies several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes.					
33454012	7	52	theme	highest	1372:1378	arg1	efficiency					1390:1399	the highest catalytic efficiency	1368:1399	the highest catalytic efficiency	1368:1399	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	53	theme	cellulolytic	1221:1232	arg1	microbes					1234:1241	cellulolytic microbes	1221:1241	cellulolytic microbes	1221:1241	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	2	54	theme	GHs	379:381	arg1	Discovery					362:370	Discovery	362:370	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites	362:455	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	7	55	theme	enzymes	1208:1214	arg1	structures					1181:1190	structures	1181:1190	structures of 10 new GH5_4 enzymes from cellulolytic microbes	1181:1241	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	9	56	theme	broad	1807:1811	arg1	specificity					1813:1823	broad specificity	1807:1823	broad specificity	1807:1823	Our new composite model identifies several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes.					
33454012	7	57	from	structures	1181:1190	arg1	microbes					1234:1241	cellulolytic microbes	1221:1241	cellulolytic microbes	1221:1241	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	58	theme	new	1198:1200	arg1	enzymes					1208:1214	10 new GH5_4 enzymes	1195:1214	10 new GH5_4 enzymes from cellulolytic microbes	1195:1241	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	5	59	theme	broad	933:937	arg1	specificity					939:949	broad specificity	933:949	broad specificity	933:949	We previously surveyed the whole subfamily and found over 100 new broad-specificity endoglucanases, although the structural origins of broad specificity remained unclear.					
33454012	6	60	theme	protein	1055:1061	arg1	design					1063:1068	the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases	1046:1163	design	1063:1068	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	4	61	theme	numerous	691:698	arg1	endoglucanases					718:731	numerous broad-selectivity endoglucanases	691:731	numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans	691:795	Among these, subfamily 4 (GH5_4) contains numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans.					
33454012	0	62	theme	biomass	130:136	arg1	hydrolysis					138:147	biomass hydrolysis	130:147	biomass hydrolysis	130:147	A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.					
33454012	1	63	theme	plant	209:213	arg1	hydrolysis					223:232	plant biomass hydrolysis	209:232	plant biomass hydrolysis	209:232	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	8	64	theme	aromatic	1560:1567	arg1	residues					1569:1576	key aromatic residues	1556:1576	key aromatic residues	1556:1576	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	3	65	theme	-linked	613:619	arg1	oligo-					621:626	oligo-	621:626	oligo-	621:626	GH5 has over 50 subfamilies exhibiting selectivities for reaction with β-(1,4)-linked oligo- and polysaccharides.					
33454012	6	66	theme	specificity	1016:1026	arg1	understanding					983:995	A mechanistic understanding	969:995	A mechanistic understanding of GH5_4 substrate specificity	969:1026	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	8	67	theme	overall	1592:1598	arg1	rate					1609:1612	the overall reaction rate	1588:1612	the overall reaction rate	1588:1612	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	7	68	theme	secondary	1486:1494	arg1	reactions					1496:1504	opportunistic secondary reactions	1472:1504	opportunistic secondary reactions on cellulose, mannan, and xylan	1472:1536	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	1	69	theme	Broad-specificity	150:166	arg1	GHs					190:192	GHs	190:192	GHs	190:192	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	1	69	theme	Broad-specificity	150:166	arg1	hydrolases					178:187	Broad-specificity glycoside hydrolases	150:187	Broad-specificity glycoside hydrolases (GHs)	150:193	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	6	70	theme	appropriate	1094:1104	arg1	application					1117:1127	the most appropriate industrial application	1085:1127	the most appropriate industrial application of broad-specificity endoglucanases	1085:1163	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	0	71	theme	broad	80:84	arg1	specificity					96:106	broad substrate specificity	80:106	broad substrate specificity	80:106	A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.					
33454012	8	72	theme	substrate	1629:1637	arg1	tolerance					1639:1647	substrate tolerance	1629:1647	substrate tolerance	1629:1647	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	0	73	theme	specificity	96:106	arg1	determinants					64:75	determinants	64:75	determinants of broad substrate specificity	64:106	A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.					
33454012	0	73	theme	specificity	96:106	arg1	opportunities					112:124	opportunities	112:124	opportunities for biomass hydrolysis	112:147	A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.					
33454012	10	74	contain	have	1934:1937	arg2	specificity					1945:1955	broad specificity	1939:1955	broad specificity	1939:1955	We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.					
33454012	10	74	contain	have	1934:1937	arg1	endoglucanases					1915:1928	GH5_4 endoglucanases	1909:1928	GH5_4 endoglucanases	1909:1928	We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.					
33454012	7	75	theme	sugar	1314:1318	arg1	assays					1320:1325	normalized reducing sugar assays	1294:1325	normalized reducing sugar assays	1294:1325	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	7	76	theme	soluble	1448:1454	arg1	β-glucans					1456:1464	xyloglucan, glucomannan, and soluble β-glucans	1419:1464	xyloglucan, glucomannan, and soluble β-glucans	1419:1464	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	0	77	theme	structural	2:11	arg1	survey					25:30	A structural and kinetic survey	0:30	A structural and kinetic survey of GH5_4 endoglucanases	0:54	A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.					
33454012	10	78	theme	biomass	2031:2037	arg1	toolset					2054:2060	the biomass deconstruction toolset	2027:2060	the biomass deconstruction toolset	2027:2060	We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.					
33454012	4	79	theme	mixed-linkage	775:787	arg1	glucans					789:795	mixed-linkage glucans	775:795	mixed-linkage glucans	775:795	Among these, subfamily 4 (GH5_4) contains numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans.					
33454012	2	80	theme	kinetic	397:403	arg1	parameters					405:414	improved kinetic parameters	388:414	improved kinetic parameters	388:414	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	0	81	theme	kinetic	17:23	arg1	survey					25:30	A structural and kinetic survey	0:30	A structural and kinetic survey of GH5_4 endoglucanases	0:54	A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.					
33454012	1	82	theme	useful	295:300	arg1	catalysts					302:310	useful catalysts	295:310	useful catalysts	295:310	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	6	83	theme	broad-specificity	1132:1148	arg1	endoglucanases					1150:1163	broad-specificity endoglucanases	1132:1163	broad-specificity endoglucanases	1132:1163	A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases.					
33454012	2	84	theme	production	510:519	arg1	efficiency					476:485	the efficiency	472:485	the efficiency of renewable bioenergy production	472:519	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	8	85	theme	cleavage	1704:1711	arg1	patterns					1713:1720	oligosaccharide cleavage patterns	1688:1720	oligosaccharide cleavage patterns	1688:1720	The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns.					
33454012	2	86	theme	tolerant	424:431	arg1	sites					451:455	more tolerant substrate-binding sites	419:455	more tolerant substrate-binding sites	419:455	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	3	87	with	reaction	592:599	arg1	polysaccharides					632:646	polysaccharides	632:646	polysaccharides	632:646	GH5 has over 50 subfamilies exhibiting selectivities for reaction with β-(1,4)-linked oligo- and polysaccharides.					
33454012	3	87	with	reaction	592:599	arg1	oligo-					621:626	oligo-	621:626	oligo-	621:626	GH5 has over 50 subfamilies exhibiting selectivities for reaction with β-(1,4)-linked oligo- and polysaccharides.					
33454012	2	88	with	Discovery	362:370	arg1	sites					451:455	more tolerant substrate-binding sites	419:455	more tolerant substrate-binding sites	419:455	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	2	88	with	Discovery	362:370	arg1	parameters					405:414	improved kinetic parameters	388:414	improved kinetic parameters	388:414	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	9	89	theme	composite	1731:1739	arg1	model					1741:1745	Our new composite model	1723:1745	Our new composite model	1723:1745	Our new composite model identifies several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes.					
33454012	2	90	theme	renewable	490:498	arg1	production					510:519	renewable bioenergy production	490:519	renewable bioenergy production	490:519	Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further.					
33454012	1	91	theme	biocommodity	337:348	arg1	production					350:359	biocommodity production	337:359	biocommodity production	337:359	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production.					
33454012	10	92	theme	high	1977:1980	arg1	activity					1982:1989	high activity	1977:1989	high activity	1977:1989	We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.					
33454012	7	93	theme	catalytic	1380:1388	arg1	efficiency					1390:1399	the highest catalytic efficiency	1368:1399	the highest catalytic efficiency	1368:1399	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
33454012	10	94	theme	deconstruction	2039:2052	arg1	toolset					2054:2060	the biomass deconstruction toolset	2027:2060	the biomass deconstruction toolset	2027:2060	We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.					
33454012	9	95	theme	critical	1766:1773	arg1	features					1786:1793	several critical structural features	1758:1793	several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes	1758:1886	Our new composite model identifies several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes.					
33454012	7	96	theme	normalized	1294:1303	arg1	assays					1320:1325	normalized reducing sugar assays	1294:1325	normalized reducing sugar assays	1294:1325	Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan.					
34226411	7	0	theme	porous	949:954	arg1	structures					979:988	scattered, fluffy, porous cellular network flake structures	930:988	structures	979:988	Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures.					
34226411	0	1	theme	Leuconostoc	97:107	arg1	mesenteroides					109:121	Potential Probiotic Leuconostoc mesenteroides LM187	77:127	Potential Probiotic Leuconostoc mesenteroides LM187	77:127	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	8	2	theme	strong	1022:1027	arg1	activity					1050:1057	strong in vitro antioxidant activity	1022:1057	strong in vitro antioxidant activity	1022:1057	In addition, rEPS414 exhibited strong in vitro antioxidant activity.					
34226411	0	3	from	Characterization	0:15	arg1	mesenteroides					109:121	Potential Probiotic Leuconostoc mesenteroides LM187	77:127	Potential Probiotic Leuconostoc mesenteroides LM187	77:127	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	4	4	theme	monosaccharide	483:496	arg1	composition					498:508	the monosaccharide composition	479:508	the monosaccharide composition	479:508	Analysis of the monosaccharide composition revealed that the molecule was mainly composed of glucose.					
34226411	1	5	with	strain	176:181	arg1	resistance					228:237	bile salt resistance	218:237	bile salt resistance	218:237	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	1	5	with	strain	176:181	arg1	resistance					206:215	good acid resistance	196:215	good acid resistance	196:215	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	1	5	with	strain	176:181	arg1	properties					265:274	cholesterol-lowering properties	244:274	cholesterol-lowering properties	244:274	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	5	6	theme	α-type	644:649	arg1	bonds					673:677	both α-type and β-type glycosidic bonds	639:677	both α-type and β-type glycosidic bonds	639:677	The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds.					
34226411	6	7	theme	purified	751:758	arg1	rEPS					760:763	the purified rEPS	747:763	the purified rEPS	747:763	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	7	8	theme	cellular	956:963	arg1	structures					979:988	scattered, fluffy, porous cellular network flake structures	930:988	structures	979:988	Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures.					
34226411	3	9	theme	purified	379:386	arg1	rEPS					388:391	The purified rEPS	375:391	The purified rEPS	375:391	The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 105 Da.					
34226411	5	10	theme	β-type	655:660	arg1	bonds					673:677	both α-type and β-type glycosidic bonds	639:677	both α-type and β-type glycosidic bonds	639:677	The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds.					
34226411	8	11	theme	in	1029:1030	arg1	activity					1050:1057	strong in vitro antioxidant activity	1022:1057	strong in vitro antioxidant activity	1022:1057	In addition, rEPS414 exhibited strong in vitro antioxidant activity.					
34226411	7	12	theme	large	914:918	arg1	number					920:925	a large number	912:925	a large number of scattered, fluffy, porous cellular network flake structures	912:988	Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures.					
34226411	3	13	theme	uniform	423:429	arg1	weight					441:446	a uniform molecular weight	421:446	a uniform molecular weight of 7.757 × 105 Da	421:464	The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 105 Da.					
34226411	3	14	theme	molecular	431:439	arg1	weight					441:446	a uniform molecular weight	421:446	a uniform molecular weight of 7.757 × 105 Da	421:464	The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 105 Da.					
34226411	6	15	theme	uronic	820:825	arg1	acid					827:830	less uronic acid	815:830	less uronic acid	815:830	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	4	16	theme	composition	498:508	arg1	Analysis					467:474	Analysis	467:474	Analysis of the monosaccharide composition	467:508	Analysis of the monosaccharide composition revealed that the molecule was mainly composed of glucose.					
34226411	8	17	theme	antioxidant	1038:1048	arg1	activity					1050:1057	strong in vitro antioxidant activity	1022:1057	strong in vitro antioxidant activity	1022:1057	In addition, rEPS414 exhibited strong in vitro antioxidant activity.					
34226411	0	18	dep	mesenteroides	109:121	arg1	LM187					123:127	LM187	123:127	LM187	123:127	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	1	19	theme	good	196:199	arg1	resistance					206:215	good acid resistance	196:215	good acid resistance	196:215	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	7	20	theme	Scanning	833:840	arg1	microscopy					851:860	Scanning electron microscopy	833:860	Scanning electron microscopy	833:860	Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures.					
34226411	3	21	dep	weight	441:446	arg1	Da					463:464	× 105 Da	457:464	a uniform molecular weight of 7.757 × 105 Da	421:464	The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 105 Da.					
34226411	3	22	contain	had	417:419	arg2	weight					441:446	a uniform molecular weight	421:446	a uniform molecular weight of 7.757 × 105 Da	421:464	The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 105 Da.					
34226411	3	22	contain	had	417:419	arg1	rEPS					388:391	The purified rEPS	375:391	The purified rEPS	375:391	The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 105 Da.					
34226411	6	23	theme	magnetic	699:706	arg1	resonance					708:716	nuclear magnetic resonance	691:716	nuclear magnetic resonance spectra	691:724	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	1	24	theme	acid	201:204	arg1	resistance					206:215	good acid resistance	196:215	good acid resistance	196:215	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	7	25	theme	network	965:971	arg1	structures					979:988	scattered, fluffy, porous cellular network flake structures	930:988	structures	979:988	Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures.					
34226411	7	26	theme	flake	973:977	arg1	structures					979:988	scattered, fluffy, porous cellular network flake structures	930:988	structures	979:988	Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures.					
34226411	8	27	dep	in	1029:1030	arg1	vitro					1032:1036	vitro	1032:1036	vitro	1032:1036	In addition, rEPS414 exhibited strong in vitro antioxidant activity.					
34226411	0	28	theme	Antioxidant	21:31	arg1	Activity					33:40	Antioxidant Activity	21:40	Antioxidant Activity	21:40	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	9	29	theme	strain	1086:1091	arg1	probiotics					1126:1135	promising probiotics	1116:1135	promising probiotics with broad prospects in industry	1116:1168	These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.					
34226411	9	29	theme	strain	1086:1091	arg1	LM187					1093:1097	strain LM187	1086:1097	strain LM187	1086:1097	These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.					
34226411	9	29	theme	strain	1086:1091	arg1	rEPS					1107:1110	its rEPS	1103:1110	its rEPS	1103:1110	These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.					
34226411	0	30	from	mesenteroides	109:121	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	0	30	from	mesenteroides	109:121	arg1	Activity					33:40	Antioxidant Activity	21:40	Antioxidant Activity	21:40	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	0	30	from	mesenteroides	109:121	arg1	Exopolysaccharide					54:70	Released Exopolysaccharide	45:70	Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187	45:127	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	1	31	theme	released	132:139	arg1	strain					176:181	A released exopolysaccharide (rEPS)-producing strain (LM187)	130:189	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties	130:274	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	1	31	theme	released	132:139	arg1	subsp					353:357	Leuconostoc mesenteroides subsp	327:357	Leuconostoc mesenteroides subsp	327:357	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	6	32	theme	resonance	708:716	arg1	spectra					718:724	nuclear magnetic resonance spectra	691:724	nuclear magnetic resonance spectra	691:724	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	9	33	theme	broad	1142:1146	arg1	prospects					1148:1156	broad prospects	1142:1156	broad prospects in industry	1142:1168	These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.					
34226411	0	34	theme	Released	45:52	arg1	Exopolysaccharide					54:70	Released Exopolysaccharide	45:70	Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187	45:127	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	9	35	theme	promising	1116:1124	arg1	probiotics					1126:1135	promising probiotics	1116:1135	promising probiotics with broad prospects in industry	1116:1168	These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.					
34226411	9	35	theme	promising	1116:1124	arg1	LM187					1093:1097	strain LM187	1086:1097	strain LM187	1086:1097	These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.					
34226411	9	35	theme	promising	1116:1124	arg1	rEPS					1107:1110	its rEPS	1103:1110	its rEPS	1103:1110	These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.					
34226411	3	36	theme	7.757	451:455	arg1	weight					441:446	a uniform molecular weight	421:446	a uniform molecular weight of 7.757 × 105 Da	421:464	The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 105 Da.					
34226411	1	37	theme	bile	218:221	arg1	resistance					228:237	bile salt resistance	218:237	bile salt resistance	218:237	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	6	38	contain	contained	765:773	arg2	rhamnose					801:808	rhamnose	801:808	rhamnose	801:808	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	6	38	contain	contained	765:773	arg1	rEPS					760:763	the purified rEPS	747:763	the purified rEPS	747:763	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	6	38	contain	contained	765:773	arg2	arabinose					775:783	arabinose	775:783	arabinose	775:783	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	6	38	contain	contained	765:773	arg2	galactose					786:794	galactose	786:794	galactose	786:794	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	6	38	contain	contained	765:773	arg2	acid					827:830	less uronic acid	815:830	less uronic acid	815:830	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	1	39	theme	salt	223:226	arg1	resistance					228:237	bile salt resistance	218:237	bile salt resistance	218:237	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	1	40	theme	Leuconostoc	327:337	arg1	mesenteroides					339:351	Leuconostoc mesenteroides	327:351	Leuconostoc mesenteroides subsp	327:357	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	9	41	from	prospects	1148:1156	arg1	industry					1161:1168	industry	1161:1168	industry	1161:1168	These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.					
34226411	7	42	theme	electron	842:849	arg1	microscopy					851:860	Scanning electron microscopy	833:860	Scanning electron microscopy	833:860	Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures.					
34226411	1	43	theme	exopolysaccharide	141:157	arg1	strain					176:181	A released exopolysaccharide (rEPS)-producing strain (LM187)	130:189	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties	130:274	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	1	43	theme	exopolysaccharide	141:157	arg1	subsp					353:357	Leuconostoc mesenteroides subsp	327:357	Leuconostoc mesenteroides subsp	327:357	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	0	44	theme	Exopolysaccharide	54:70	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	0	44	theme	Exopolysaccharide	54:70	arg1	Activity					33:40	Antioxidant Activity	21:40	Antioxidant Activity	21:40	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	1	45	theme	mesenteroides	339:351	arg1	strain					176:181	A released exopolysaccharide (rEPS)-producing strain (LM187)	130:189	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties	130:274	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	1	45	theme	mesenteroides	339:351	arg1	subsp					353:357	Leuconostoc mesenteroides subsp	327:357	Leuconostoc mesenteroides subsp	327:357	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	9	46	with	probiotics	1126:1135	arg1	prospects					1148:1156	broad prospects	1142:1156	broad prospects in industry	1142:1168	These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.					
34226411	5	47	theme	glycosidic	662:671	arg1	bonds					673:677	both α-type and β-type glycosidic bonds	639:677	both α-type and β-type glycosidic bonds	639:677	The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds.					
34226411	5	48	theme	Fourier	573:579	arg1	spectrum					600:607	The Fourier transform-infrared spectrum	569:607	The Fourier transform-infrared spectrum	569:607	The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds.					
34226411	6	49	theme	nuclear	691:697	arg1	resonance					708:716	nuclear magnetic resonance	691:716	nuclear magnetic resonance spectra	691:724	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	7	50	theme	structures	979:988	arg1	number					920:925	a large number	912:925	a large number of scattered, fluffy, porous cellular network flake structures	912:988	Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures.					
34226411	5	51	theme	transform-infrared	581:598	arg1	spectrum					600:607	The Fourier transform-infrared spectrum	569:607	The Fourier transform-infrared spectrum	569:607	The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds.					
34226411	1	52	theme	rEPS	160:163	arg1	strain					176:181	A released exopolysaccharide (rEPS)-producing strain (LM187)	130:189	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties	130:274	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	1	52	theme	rEPS	160:163	arg1	subsp					353:357	Leuconostoc mesenteroides subsp	327:357	Leuconostoc mesenteroides subsp	327:357	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	6	53	theme	13C	687:689	arg1	analysis					726:733	1H and 13C nuclear magnetic resonance spectra analysis	680:733	1H and 13C nuclear magnetic resonance spectra analysis	680:733	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	7	54	dep	scattered	930:938	arg1	fluffy					941:946	fluffy	941:946	fluffy	941:946	Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures.					
34226411	5	55	contain	contained	629:637	arg1	rEPS414					621:627	rEPS414	621:627	rEPS414	621:627	The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds.					
34226411	5	55	contain	contained	629:637	arg2	bonds					673:677	both α-type and β-type glycosidic bonds	639:677	both α-type and β-type glycosidic bonds	639:677	The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds.					
34226411	0	56	theme	Probiotic	87:95	arg1	mesenteroides					109:121	Potential Probiotic Leuconostoc mesenteroides LM187	77:127	Potential Probiotic Leuconostoc mesenteroides LM187	77:127	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	6	57	dep	1H	680:681	arg1	spectra					718:724	nuclear magnetic resonance spectra	691:724	nuclear magnetic resonance spectra	691:724	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	1	58	theme	-producing	165:174	arg1	strain					176:181	A released exopolysaccharide (rEPS)-producing strain (LM187)	130:189	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties	130:274	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	1	58	theme	-producing	165:174	arg1	subsp					353:357	Leuconostoc mesenteroides subsp	327:357	Leuconostoc mesenteroides subsp	327:357	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	6	59	theme	1H	680:681	arg1	analysis					726:733	1H and 13C nuclear magnetic resonance spectra analysis	680:733	1H and 13C nuclear magnetic resonance spectra analysis	680:733	1H and 13C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid.					
34226411	0	60	theme	Potential	77:85	arg1	mesenteroides					109:121	Potential Probiotic Leuconostoc mesenteroides LM187	77:127	Potential Probiotic Leuconostoc mesenteroides LM187	77:127	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	0	61	from	Activity	33:40	arg1	mesenteroides					109:121	Potential Probiotic Leuconostoc mesenteroides LM187	77:127	Potential Probiotic Leuconostoc mesenteroides LM187	77:127	Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187.					
34226411	1	62	attach	isolated	280:287	arg2	subsp					353:357	Leuconostoc mesenteroides subsp	327:357	Leuconostoc mesenteroides subsp	327:357	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	1	62	attach	isolated	280:287	arg2	strain					176:181	A released exopolysaccharide (rEPS)-producing strain (LM187)	130:189	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties	130:274	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	1	62	attach	isolated	280:287	arg1	paocai					302:307	paocai	302:307	paocai	302:307	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
34226411	7	63	theme	scattered	930:938	arg1	structures					979:988	scattered, fluffy, porous cellular network flake structures	930:988	structures	979:988	Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures.					
34226411	1	64	theme	cholesterol-lowering	244:263	arg1	properties					265:274	cholesterol-lowering properties	244:274	cholesterol-lowering properties	244:274	A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp.					
33857512	1	0	theme	dosage	159:164	arg1	Astragalus					143:152	Honey-processed Astragalus	127:152	Honey-processed Astragalus	127:152	Honey-processed Astragalus is a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product.					
33857512	1	0	theme	dosage	159:164	arg1	form					166:169	a dosage form	157:169	a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product	157:278	Honey-processed Astragalus is a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product.					
33857512	1	1	theme	tonifying	246:254	arg1	Qi					256:257	tonifying Qi	246:257	tonifying Qi	246:257	Honey-processed Astragalus is a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product.					
33857512	0	2	from	differences	11:21	arg1	Astragalus					47:56	Astragalus	47:56	Astragalus before and after honey processing	47:90	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	0	2	from	differences	11:21	arg1	mice					121:124	colitis mice	113:124	colitis mice	113:124	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	3	3	from	effects	518:524	arg1	mice					537:540	colitis mice	529:540	colitis mice	529:540	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	7	4	theme	chain	1121:1125	arg1	conformation					1127:1138	the chain conformation	1117:1138	the chain conformation	1117:1138	The different DB of HAPS3a (15.35%) and APS3a (25.13%) suggested that the chain conformation became smoother.					
33857512	1	5	theme	Qi	256:257	arg1	efficacy					234:241	better efficacy	227:241	better efficacy of tonifying Qi than the raw product	227:278	Honey-processed Astragalus is a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product.					
33857512	4	6	theme	branching	710:718	arg1	compositions					665:676	monosaccharide compositions	650:676	monosaccharide compositions	650:676	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	4	6	theme	branching	710:718	arg1	bonds					690:694	glycosidic bonds	679:694	glycosidic bonds	679:694	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	4	6	theme	branching	710:718	arg1	degree					700:705	degree	700:705	degree of branching (DB)	700:723	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	4	6	theme	branching	710:718	arg1	weight					642:647	molecular weight	632:647	molecular weight	632:647	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	9	7	from	differences	1390:1400	arg1	activity					1423:1430	anti-inflammatory activity	1405:1430	anti-inflammatory activity	1405:1430	Taken together, the differences in anti-inflammatory activity might be related to structural differences caused by honey processing.					
33857512	8	8	theme	better	1234:1239	arg1	effects					1241:1247	better effects	1234:1247	better effects	1234:1247	The anti-inflammatory effects on colitis mice revealed that HAPS3a exhibited better effects than APS3a by protecting intestinal mucosa, regulating the expression of cytokines and influencing microbiota diversity.					
33857512	6	9	theme	acid	891:894	arg1	1,4-β-GalpA					905:915	1,4-β-GalpA	905:915	1,4-β-GalpA	905:915	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	9	theme	acid	891:894	arg1	residues					896:903	The uronic acid residues	880:903	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a	880:956	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	9	theme	acid	891:894	arg1	1,6-α-GlcpA					921:931	1,6-α-GlcpA	921:931	1,6-α-GlcpA	921:931	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	9	theme	acid	891:894	arg1	backbone					940:947	the backbone	936:947	the backbone	936:947	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	0	10	theme	colitis	113:119	arg1	mice					121:124	colitis mice	113:124	colitis mice	113:124	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	6	11	from	residues	896:903	arg1	APS3a					952:956	APS3a	952:956	APS3a	952:956	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	8	12	from	effects	1179:1185	arg1	mice					1198:1201	colitis mice	1190:1201	colitis mice	1190:1201	The anti-inflammatory effects on colitis mice revealed that HAPS3a exhibited better effects than APS3a by protecting intestinal mucosa, regulating the expression of cytokines and influencing microbiota diversity.					
33857512	1	13	theme	Astragali	180:188	arg1	Astragalus					143:152	Honey-processed Astragalus	127:152	Honey-processed Astragalus	127:152	Honey-processed Astragalus is a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product.					
33857512	1	13	theme	Astragali	180:188	arg1	form					166:169	a dosage form	157:169	a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product	157:278	Honey-processed Astragalus is a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product.					
33857512	6	14	theme	neutral	996:1002	arg1	residues					1004:1011	the corresponding neutral residues	978:1011	the corresponding neutral residues in HAPS3a	978:1021	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	7	15	theme	APS3a	1087:1091	arg1	DB					1061:1062	The different DB	1047:1062	The different DB of HAPS3a (15.35%) and APS3a (25.13%)	1047:1100	The different DB of HAPS3a (15.35%) and APS3a (25.13%) suggested that the chain conformation became smoother.					
33857512	0	16	from	Astragalus	47:56	arg1	effects					102:108	their effects	96:108	their effects on colitis mice	96:124	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	0	16	from	Astragalus	47:56	arg1	polysaccharides					26:40	polysaccharides	26:40	polysaccharides from Astragalus before and after honey processing	26:90	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	0	16	from	Astragalus	47:56	arg1	differences					11:21	Structural differences	0:21	Structural differences of polysaccharides from Astragalus before and after honey processing	0:90	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	9	17	theme	honey	1485:1489	arg1	processing					1491:1500	honey processing	1485:1500	honey processing	1485:1500	Taken together, the differences in anti-inflammatory activity might be related to structural differences caused by honey processing.					
33857512	6	18	theme	corresponding	982:994	arg1	residues					1004:1011	the corresponding neutral residues	978:1011	the corresponding neutral residues in HAPS3a	978:1021	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	3	19	theme	structural	379:388	arg1	differences					390:400	the structural differences	375:400	the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a)	375:506	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	3	20	from	differences	390:400	arg1	mice					537:540	colitis mice	529:540	colitis mice	529:540	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	6	21	theme	uronic	884:889	arg1	1,4-β-GalpA					905:915	1,4-β-GalpA	905:915	1,4-β-GalpA	905:915	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	21	theme	uronic	884:889	arg1	residues					896:903	The uronic acid residues	880:903	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a	880:956	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	21	theme	uronic	884:889	arg1	1,6-α-GlcpA					921:931	1,6-α-GlcpA	921:931	1,6-α-GlcpA	921:931	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	21	theme	uronic	884:889	arg1	backbone					940:947	the backbone	936:947	the backbone	936:947	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	2	22	theme	water-soluble	310:322	arg1	Polysaccharides					281:295	Polysaccharides	281:295	Polysaccharides	281:295	Polysaccharides are its main water-soluble active components.					
33857512	2	22	theme	water-soluble	310:322	arg1	components					331:340	its main water-soluble active components	301:340	its main water-soluble active components	301:340	Polysaccharides are its main water-soluble active components.					
33857512	3	23	theme	polysaccharides	444:458	arg1	differences					390:400	the structural differences	375:400	the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a)	375:506	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	3	23	theme	polysaccharides	444:458	arg1	effects					518:524	their effects	512:524	their effects on colitis mice	512:540	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	0	24	theme	Structural	0:9	arg1	differences					11:21	Structural differences	0:21	Structural differences of polysaccharides from Astragalus before and after honey processing	0:90	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	1	25	theme	raw	268:270	arg1	product					272:278	the raw product	264:278	the raw product	264:278	Honey-processed Astragalus is a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product.					
33857512	2	26	theme	main	305:308	arg1	Polysaccharides					281:295	Polysaccharides	281:295	Polysaccharides	281:295	Polysaccharides are its main water-soluble active components.					
33857512	2	26	theme	main	305:308	arg1	components					331:340	its main water-soluble active components	301:340	its main water-soluble active components	301:340	Polysaccharides are its main water-soluble active components.					
33857512	4	27	theme	molecular	632:640	arg1	weight					642:647	molecular weight	632:647	molecular weight	632:647	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	0	28	theme	polysaccharides	26:40	arg1	effects					102:108	their effects	96:108	their effects on colitis mice	96:124	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	0	28	theme	polysaccharides	26:40	arg1	differences					11:21	Structural differences	0:21	Structural differences of polysaccharides from Astragalus before and after honey processing	0:90	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	8	29	theme	colitis	1190:1196	arg1	mice					1198:1201	colitis mice	1190:1201	colitis mice	1190:1201	The anti-inflammatory effects on colitis mice revealed that HAPS3a exhibited better effects than APS3a by protecting intestinal mucosa, regulating the expression of cytokines and influencing microbiota diversity.					
33857512	10	30	theme	Astragalus	1571:1580	arg1	mechanism					1558:1566	the processing mechanism	1543:1566	the processing mechanism of Astragalus	1543:1580	Our findings have laid a foundation for the processing mechanism of Astragalus.					
33857512	3	31	theme	polysaccharides	484:498	arg1	differences					390:400	the structural differences	375:400	the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a)	375:506	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	3	31	theme	polysaccharides	484:498	arg1	effects					518:524	their effects	512:524	their effects on colitis mice	512:540	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	5	32	theme	acid	782:785	arg1	ratios					745:750	the molar ratios	735:750	the molar ratios of galactose and galacturonic acid in HAPS3a	735:795	Notably, the molar ratios of galactose and galacturonic acid in HAPS3a were 22.66% and 33.24%, while those in APS3a were 11.87% and 49.55%, respectively.					
33857512	5	32	theme	acid	782:785	arg1	%					807:807	22.66%	802:807	22.66%	802:807	Notably, the molar ratios of galactose and galacturonic acid in HAPS3a were 22.66% and 33.24%, while those in APS3a were 11.87% and 49.55%, respectively.					
33857512	8	33	theme	intestinal	1274:1283	arg1	mucosa					1285:1290	intestinal mucosa	1274:1290	intestinal mucosa	1274:1290	The anti-inflammatory effects on colitis mice revealed that HAPS3a exhibited better effects than APS3a by protecting intestinal mucosa, regulating the expression of cytokines and influencing microbiota diversity.					
33857512	7	34	theme	HAPS3a	1067:1072	arg1	DB					1061:1062	The different DB	1047:1062	The different DB of HAPS3a (15.35%) and APS3a (25.13%)	1047:1100	The different DB of HAPS3a (15.35%) and APS3a (25.13%) suggested that the chain conformation became smoother.					
33857512	4	35	theme	monosaccharide	650:663	arg1	compositions					665:676	monosaccharide compositions	650:676	monosaccharide compositions	650:676	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	3	36	theme	honey-processed	417:431	arg1	HAPS3a					461:466	HAPS3a	461:466	HAPS3a	461:466	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	3	36	theme	honey-processed	417:431	arg1	polysaccharides					444:458	homogeneous honey-processed Astragalus polysaccharides	405:458	homogeneous honey-processed Astragalus polysaccharides (HAPS3a)	405:467	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	9	37	theme	anti-inflammatory	1405:1421	arg1	activity					1423:1430	anti-inflammatory activity	1405:1430	anti-inflammatory activity	1405:1430	Taken together, the differences in anti-inflammatory activity might be related to structural differences caused by honey processing.					
33857512	8	38	theme	microbiota	1348:1357	arg1	diversity					1359:1367	microbiota diversity	1348:1367	microbiota diversity	1348:1367	The anti-inflammatory effects on colitis mice revealed that HAPS3a exhibited better effects than APS3a by protecting intestinal mucosa, regulating the expression of cytokines and influencing microbiota diversity.					
33857512	8	39	theme	anti-inflammatory	1161:1177	arg1	effects					1179:1185	The anti-inflammatory effects	1157:1185	The anti-inflammatory effects on colitis mice	1157:1201	The anti-inflammatory effects on colitis mice revealed that HAPS3a exhibited better effects than APS3a by protecting intestinal mucosa, regulating the expression of cytokines and influencing microbiota diversity.					
33857512	3	40	theme	Astragalus	433:442	arg1	HAPS3a					461:466	HAPS3a	461:466	HAPS3a	461:466	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	3	40	theme	Astragalus	433:442	arg1	polysaccharides					444:458	homogeneous honey-processed Astragalus polysaccharides	405:458	homogeneous honey-processed Astragalus polysaccharides (HAPS3a)	405:467	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	5	41	theme	molar	739:743	arg1	ratios					745:750	the molar ratios	735:750	the molar ratios of galactose and galacturonic acid in HAPS3a	735:795	Notably, the molar ratios of galactose and galacturonic acid in HAPS3a were 22.66% and 33.24%, while those in APS3a were 11.87% and 49.55%, respectively.					
33857512	5	41	theme	molar	739:743	arg1	%					807:807	22.66%	802:807	22.66%	802:807	Notably, the molar ratios of galactose and galacturonic acid in HAPS3a were 22.66% and 33.24%, while those in APS3a were 11.87% and 49.55%, respectively.					
33857512	5	42	theme	galacturonic	769:780	arg1	acid					782:785	galacturonic acid	769:785	galacturonic acid	769:785	Notably, the molar ratios of galactose and galacturonic acid in HAPS3a were 22.66% and 33.24%, while those in APS3a were 11.87% and 49.55%, respectively.					
33857512	3	43	theme	Astragalus	473:482	arg1	APS3a					501:505	APS3a	501:505	APS3a	501:505	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	3	43	theme	Astragalus	473:482	arg1	polysaccharides					484:498	Astragalus polysaccharides	473:498	Astragalus polysaccharides (APS3a)	473:506	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	7	44	theme	different	1051:1059	arg1	DB					1061:1062	The different DB	1047:1062	The different DB of HAPS3a (15.35%) and APS3a (25.13%)	1047:1100	The different DB of HAPS3a (15.35%) and APS3a (25.13%) suggested that the chain conformation became smoother.					
33857512	4	45	dep	=	578:578	arg1	kDa					587:589	2463.5 kDa	580:589	2463.5 kDa	580:589	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	4	46	dep	HAPS3a	567:572	arg1	Mw					603:604	Mw	603:604	Mw	603:604	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	4	46	dep	HAPS3a	567:572	arg1	Mw					575:576	Mw	575:576	Mw	575:576	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	3	47	theme	homogeneous	405:415	arg1	HAPS3a					461:466	HAPS3a	461:466	HAPS3a	461:466	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	3	47	theme	homogeneous	405:415	arg1	polysaccharides					444:458	homogeneous honey-processed Astragalus polysaccharides	405:458	homogeneous honey-processed Astragalus polysaccharides (HAPS3a)	405:467	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	9	48	theme	structural	1452:1461	arg1	differences					1463:1473	structural differences	1452:1473	structural differences caused by honey processing	1452:1500	Taken together, the differences in anti-inflammatory activity might be related to structural differences caused by honey processing.					
33857512	6	49	dep	residues	896:903	arg1	1,4-β-GalpA					905:915	1,4-β-GalpA	905:915	1,4-β-GalpA	905:915	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	49	dep	residues	896:903	arg1	residues					896:903	The uronic acid residues	880:903	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a	880:956	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	49	dep	residues	896:903	arg1	1,6-α-GlcpA					921:931	1,6-α-GlcpA	921:931	1,6-α-GlcpA	921:931	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	49	dep	residues	896:903	arg1	backbone					940:947	the backbone	936:947	the backbone	936:947	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	4	50	dep	=	606:606	arg1	kDa					615:617	3373.2 kDa	608:617	3373.2 kDa	608:617	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	4	51	theme	glycosidic	679:688	arg1	bonds					690:694	glycosidic bonds	679:694	glycosidic bonds	679:694	The results showed that HAPS3a (Mw = 2463.5 kDa) and APS3a (Mw = 3373.2 kDa) differed in molecular weight, monosaccharide compositions, glycosidic bonds and degree of branching (DB).					
33857512	8	52	theme	cytokines	1322:1330	arg1	expression					1308:1317	the expression	1304:1317	the expression of cytokines	1304:1330	The anti-inflammatory effects on colitis mice revealed that HAPS3a exhibited better effects than APS3a by protecting intestinal mucosa, regulating the expression of cytokines and influencing microbiota diversity.					
33857512	1	53	theme	Honey-processed	127:141	arg1	Astragalus					143:152	Honey-processed Astragalus	127:152	Honey-processed Astragalus	127:152	Honey-processed Astragalus is a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product.					
33857512	1	53	theme	Honey-processed	127:141	arg1	form					166:169	a dosage form	157:169	a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product	157:278	Honey-processed Astragalus is a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product.					
33857512	6	54	theme	honey	1029:1033	arg1	processing					1035:1044	honey processing	1029:1044	honey processing	1029:1044	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	55	theme	backbone	940:947	arg1	1,4-β-GalpA					905:915	1,4-β-GalpA	905:915	1,4-β-GalpA	905:915	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	55	theme	backbone	940:947	arg1	residues					896:903	The uronic acid residues	880:903	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a	880:956	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	55	theme	backbone	940:947	arg1	1,6-α-GlcpA					921:931	1,6-α-GlcpA	921:931	1,6-α-GlcpA	921:931	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	6	55	theme	backbone	940:947	arg1	backbone					940:947	the backbone	936:947	the backbone	936:947	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	0	56	from	effects	102:108	arg1	Astragalus					47:56	Astragalus	47:56	Astragalus before and after honey processing	47:90	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	0	56	from	effects	102:108	arg1	mice					121:124	colitis mice	113:124	colitis mice	113:124	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	2	57	theme	active	324:329	arg1	Polysaccharides					281:295	Polysaccharides	281:295	Polysaccharides	281:295	Polysaccharides are its main water-soluble active components.					
33857512	2	57	theme	active	324:329	arg1	components					331:340	its main water-soluble active components	301:340	its main water-soluble active components	301:340	Polysaccharides are its main water-soluble active components.					
33857512	1	58	theme	better	227:232	arg1	efficacy					234:241	better efficacy	227:241	better efficacy of tonifying Qi than the raw product	227:278	Honey-processed Astragalus is a dosage form of Radix Astragali processed with honey, which exhibits better efficacy of tonifying Qi than the raw product.					
33857512	0	59	theme	honey	75:79	arg1	processing					81:90	honey processing	75:90	honey processing	75:90	Structural differences of polysaccharides from Astragalus before and after honey processing and their effects on colitis mice.					
33857512	6	60	from	residues	1004:1011	arg1	HAPS3a					1016:1021	HAPS3a	1016:1021	HAPS3a	1016:1021	The uronic acid residues 1,4-β-GalpA and 1,6-α-GlcpA of the backbone in APS3a were converted into the corresponding neutral residues in HAPS3a after honey processing.					
33857512	3	61	theme	colitis	529:535	arg1	mice					537:540	colitis mice	529:540	colitis mice	529:540	This work was designed to study the structural differences of homogeneous honey-processed Astragalus polysaccharides (HAPS3a) and Astragalus polysaccharides (APS3a) and their effects on colitis mice.					
33857512	5	62	from	ratios	745:750	arg1	HAPS3a					790:795	HAPS3a	790:795	HAPS3a	790:795	Notably, the molar ratios of galactose and galacturonic acid in HAPS3a were 22.66% and 33.24%, while those in APS3a were 11.87% and 49.55%, respectively.					
33857512	5	63	theme	galactose	755:763	arg1	ratios					745:750	the molar ratios	735:750	the molar ratios of galactose and galacturonic acid in HAPS3a	735:795	Notably, the molar ratios of galactose and galacturonic acid in HAPS3a were 22.66% and 33.24%, while those in APS3a were 11.87% and 49.55%, respectively.					
33857512	5	63	theme	galactose	755:763	arg1	%					807:807	22.66%	802:807	22.66%	802:807	Notably, the molar ratios of galactose and galacturonic acid in HAPS3a were 22.66% and 33.24%, while those in APS3a were 11.87% and 49.55%, respectively.					
33857512	10	64	theme	processing	1547:1556	arg1	mechanism					1558:1566	the processing mechanism	1543:1566	the processing mechanism of Astragalus	1543:1580	Our findings have laid a foundation for the processing mechanism of Astragalus.					
34775614	2	0	dep	non-pretreated	520:533	arg1	DO					535:536	DO	535:536	DO	535:536	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	6	1	theme	250 kDa	1298:1304	arg1	weight					1288:1293	a molecular weight	1276:1293	a molecular weight of 250 kDa	1276:1304	In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide.					
34775614	2	2	theme	crude	543:547	arg1	7.5DO-CP					582:589	7.5DO-CP	582:589	7.5DO-CP	582:589	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	2	2	theme	crude	543:547	arg1	DOOS-CP					573:579	DOOS-CP	573:579	DOOS-CP	573:579	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	2	2	theme	crude	543:547	arg1	DO-CP					566:570	DO-CP	566:570	DO-CP	566:570	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	2	2	theme	crude	543:547	arg1	9DO-CP					595:600	9DO-CP	595:600	9DO-CP	595:600	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	2	2	theme	crude	543:547	arg1	polysaccharides					549:563	crude polysaccharides	543:563	crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP)	543:601	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	8	3	theme	Chemical	1624:1631	arg1	Industry					1633:1640	Chemical Industry	1624:1640	Chemical Industry	1624:1640	© 2021 Society of Chemical Industry.					
34775614	1	4	dep	kgf	399:401	arg1	9DO					414:416	9DO	414:416	9DO	414:416	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	1	4	dep	kgf	399:401	arg1	7.5DO					404:408	7.5DO	404:408	7.5DO	404:408	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	7	5	theme	organic	1418:1424	arg1	solvents					1426:1433	organic solvents	1418:1433	organic solvents	1418:1433	CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.					
34775614	7	6	theme	D.	1399:1400	arg1	officinale					1402:1411	D. officinale	1399:1411	D. officinale	1399:1411	CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.					
34775614	1	7	theme	enhanced	182:189	arg1	activity					209:216	enhanced immunostimulatory activity	182:216	enhanced immunostimulatory activity	182:216	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	3	8	theme	immune	782:787	arg1	system					789:794	intestinal immune system	771:794	intestinal immune system	771:794	RESULTS When their immunostimulatory activities were compared by macrophage stimulation and intestinal immune system modulation via Peyer's patches, DOOS-CP showed more potent activity than DO-CP.					
34775614	4	9	theme	crude	885:889	arg1	polysaccharides					891:905	crude polysaccharides	885:905	crude polysaccharides fractionated from puffed DO	885:933	However, crude polysaccharides fractionated from puffed DO showed significantly lower activity than non-puffed DO and DOOS.					
34775614	1	10	theme	organic	349:355	arg1	DOOS					367:370	DOOS	367:370	DOOS	367:370	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	1	10	theme	organic	349:355	arg1	solvents					357:364	organic solvents	349:364	organic solvents (DOOS)	349:371	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	1	11	dep	BACKGROUND	139:148	arg1	isolate					153:159	isolate	153:159	To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO)	150:327	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	6	12	contain	containing	1238:1247	arg1	fraction					1229:1236	a somewhat refined fraction	1210:1236	a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide	1210:1342	In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide.					
34775614	6	12	contain	containing	1238:1247	arg1	DOOS-CP					1198:1204	DOOS-CP	1198:1204	DOOS-CP	1198:1204	In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide.					
34775614	6	12	contain	containing	1238:1247	arg2	peak					1257:1260	a major peak	1249:1260	a major peak	1249:1260	In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide.					
34775614	3	13	dep	RESULTS	679:685	arg1	showed					836:841	showed	836:841	showed more potent activity than DO-CP	836:873	RESULTS When their immunostimulatory activities were compared by macrophage stimulation and intestinal immune system modulation via Peyer's patches, DOOS-CP showed more potent activity than DO-CP.					
34775614	0	14	theme	Dendrobium	95:104	arg1	officinale					106:115	Dendrobium officinale	95:115	Dendrobium officinale in the pretreatments	95:136	Changes in the component sugar and immunostimulating activity of polysaccharides isolated from Dendrobium officinale in the pretreatments.					
34775614	1	15	with	polysaccharides	161:175	arg1	activity					209:216	enhanced immunostimulatory activity	182:216	enhanced immunostimulatory activity	182:216	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	3	16	theme	potent	848:853	arg1	activity					855:862	more potent activity	843:862	more potent activity	843:862	RESULTS When their immunostimulatory activities were compared by macrophage stimulation and intestinal immune system modulation via Peyer's patches, DOOS-CP showed more potent activity than DO-CP.					
34775614	5	17	theme	polysaccharide	1145:1158	arg1	2.0					1172:1174	2.0	1172:1174	2.0	1172:1174	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	5	17	theme	polysaccharide	1145:1158	arg1	content					1160:1166	the lowest polysaccharide content	1134:1166	the lowest polysaccharide content	1134:1166	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	2	18	dep	extracts	430:437	arg1	7.5DO-HW					449:456	7.5DO-HW	449:456	7.5DO-HW	449:456	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	2	18	dep	extracts	430:437	arg1	DOOS-HW					440:446	DOOS-HW	440:446	DOOS-HW	440:446	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	2	18	dep	extracts	430:437	arg1	9DO-HW					462:467	9DO-HW	462:467	9DO-HW	462:467	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	7	19	with	pretreatment	1383:1394	arg1	solvents					1426:1433	organic solvents	1418:1433	organic solvents	1418:1433	CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.					
34775614	1	20	theme	immunostimulatory	191:207	arg1	activity					209:216	enhanced immunostimulatory activity	182:216	enhanced immunostimulatory activity	182:216	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	2	21	dep	pretreated	494:503	arg1	DO					505:506	DO	505:506	DO	505:506	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	5	22	theme	neutral	1053:1059	arg1	sugar					1061:1065	more neutral sugar	1048:1065	more neutral sugar	1048:1065	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	3	23	theme	intestinal	771:780	arg1	system					789:794	intestinal immune system	771:794	intestinal immune system	771:794	RESULTS When their immunostimulatory activities were compared by macrophage stimulation and intestinal immune system modulation via Peyer's patches, DOOS-CP showed more potent activity than DO-CP.					
34775614	2	24	dep	polysaccharides	549:563	arg1	7.5DO-CP					582:589	7.5DO-CP	582:589	7.5DO-CP	582:589	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	2	24	dep	polysaccharides	549:563	arg1	DOOS-CP					573:579	DOOS-CP	573:579	DOOS-CP	573:579	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	2	24	dep	polysaccharides	549:563	arg1	DO-CP					566:570	DO-CP	566:570	DO-CP	566:570	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	2	24	dep	polysaccharides	549:563	arg1	polysaccharides					549:563	crude polysaccharides	543:563	crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP)	543:601	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	2	24	dep	polysaccharides	549:563	arg1	9DO-CP					595:600	9DO-CP	595:600	9DO-CP	595:600	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	5	25	theme	glucose	1109:1115	arg1	ratio					1088:1092	the composition ratio	1072:1092	the composition ratio of mannose and glucose	1072:1115	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	5	25	theme	glucose	1109:1115	arg1	3.0					1121:1123	3.0	1121:1123	3.0	1121:1123	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	4	26	dep	non-puffed	976:985	arg1	DO					987:988	DO	987:988	DO	987:988	However, crude polysaccharides fractionated from puffed DO showed significantly lower activity than non-puffed DO and DOOS.					
34775614	3	27	dep	stimulation	755:765	arg1	modulation					796:805	modulation	796:805	modulation	796:805	RESULTS When their immunostimulatory activities were compared by macrophage stimulation and intestinal immune system modulation via Peyer's patches, DOOS-CP showed more potent activity than DO-CP.					
34775614	0	28	theme	immunostimulating	35:51	arg1	activity					53:60	the component sugar and immunostimulating activity	11:60	activity	53:60	Changes in the component sugar and immunostimulating activity of polysaccharides isolated from Dendrobium officinale in the pretreatments.					
34775614	1	29	theme	Dendrobium	223:232	arg1	medicine					272:279	a herbal medicine	263:279	a herbal medicine in China and Southeast Asia, D. officinale	263:322	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	1	29	theme	Dendrobium	223:232	arg1	officinale					234:243	Dendrobium officinale	223:243	Dendrobium officinale	223:243	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	0	30	theme	component	15:23	arg1	sugar					25:29	the component sugar and immunostimulating activity	11:60	sugar	25:29	Changes in the component sugar and immunostimulating activity of polysaccharides isolated from Dendrobium officinale in the pretreatments.					
34775614	6	31	theme	refined	1221:1227	arg1	fraction					1229:1236	a somewhat refined fraction	1210:1236	a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide	1210:1342	In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide.					
34775614	6	31	theme	refined	1221:1227	arg1	DOOS-CP					1198:1204	DOOS-CP	1198:1204	DOOS-CP	1198:1204	In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide.					
34775614	1	32	dep	isolate	153:159	arg1	DO					325:326	DO	325:326	DO	325:326	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	4	33	theme	lower	956:960	arg1	activity					962:969	significantly lower activity	942:969	significantly lower activity than non-puffed DO and DOOS	942:997	However, crude polysaccharides fractionated from puffed DO showed significantly lower activity than non-puffed DO and DOOS.					
34775614	7	34	dep	CONCLUSION	1345:1354	arg1	suggest					1370:1376	suggest	1370:1376	suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation	1370:1603	CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.					
34775614	1	35	from	medicine	272:279	arg1	D.					310:311	D.	310:311	D.	310:311	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	1	35	from	medicine	272:279	arg1	China					284:288	China	284:288	China	284:288	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	7	36	theme	polysaccharides	1481:1495	arg1	activity					1469:1476	the immunostimulatory activity	1447:1476	the immunostimulatory activity of polysaccharides	1447:1495	CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.					
34775614	6	37	theme	crude	1323:1327	arg1	polysaccharide					1329:1342	a crude polysaccharide	1321:1342	a crude polysaccharide	1321:1342	In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide.					
34775614	3	38	theme	immunostimulatory	698:714	arg1	activities					716:725	their immunostimulatory activities	692:725	their immunostimulatory activities	692:725	RESULTS When their immunostimulatory activities were compared by macrophage stimulation and intestinal immune system modulation via Peyer's patches, DOOS-CP showed more potent activity than DO-CP.					
34775614	7	39	theme	mannose/glucose	1512:1526	arg1	ratio					1528:1532	the mannose/glucose ratio	1508:1532	the mannose/glucose ratio of polysaccharides	1508:1551	CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.					
34775614	6	40	theme	major	1251:1255	arg1	peak					1257:1260	a major peak	1249:1260	a major peak	1249:1260	In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide.					
34775614	5	41	contain	contained	1031:1039	arg2	%					1043:1043	95%	1041:1043	95%	1041:1043	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	5	41	contain	contained	1031:1039	arg2	sugar					1061:1065	more neutral sugar	1048:1065	more neutral sugar	1048:1065	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	5	41	contain	contained	1031:1039	arg1	polysaccharide					1016:1029	The most active polysaccharide	1000:1029	The most active polysaccharide	1000:1029	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	6	42	from	fraction	1229:1236	arg1	addition					1188:1195	addition	1188:1195	addition	1188:1195	In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide.					
34775614	2	43	theme	Hot-water	420:428	arg1	extracts					430:437	Hot-water extracts	420:437	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW)	420:468	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	7	44	theme	important	1569:1577	arg1	role					1579:1582	an important role	1566:1582	an important role	1566:1582	CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.					
34775614	2	45	theme	hot-water	631:639	arg1	extract					641:647	each hot-water extract	626:647	each hot-water extract using ethanol (five volumes)	626:676	Hot-water extracts (DOOS-HW, 7.5DO-HW and 9DO-HW) were prepared from each pretreated DO, along with non-pretreated DO, and crude polysaccharides (DO-CP, DOOS-CP, 7.5DO-CP and 9DO-CP) were fractionated from each hot-water extract using ethanol (five volumes).					
34775614	0	46	from	Changes	0:6	arg1	sugar					25:29	the component sugar and immunostimulating activity	11:60	sugar	25:29	Changes in the component sugar and immunostimulating activity of polysaccharides isolated from Dendrobium officinale in the pretreatments.					
34775614	0	46	from	Changes	0:6	arg1	activity					53:60	the component sugar and immunostimulating activity	11:60	activity	53:60	Changes in the component sugar and immunostimulating activity of polysaccharides isolated from Dendrobium officinale in the pretreatments.					
34775614	5	47	theme	mannose	1097:1103	arg1	ratio					1088:1092	the composition ratio	1072:1092	the composition ratio of mannose and glucose	1072:1115	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	5	47	theme	mannose	1097:1103	arg1	3.0					1121:1123	3.0	1121:1123	3.0	1121:1123	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	1	48	dep	China	284:288	arg1	officinale					313:322	officinale	313:322	officinale	313:322	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	7	49	theme	immunostimulatory	1451:1467	arg1	activity					1469:1476	the immunostimulatory activity	1447:1476	the immunostimulatory activity of polysaccharides	1447:1495	CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.					
34775614	5	50	theme	active	1009:1014	arg1	polysaccharide					1016:1029	The most active polysaccharide	1000:1029	The most active polysaccharide	1000:1029	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	4	51	dep	puffed	925:930	arg1	DO					932:933	DO	932:933	DO	932:933	However, crude polysaccharides fractionated from puffed DO showed significantly lower activity than non-puffed DO and DOOS.					
34775614	7	52	theme	officinale	1402:1411	arg1	pretreatment					1383:1394	pretreatment	1383:1394	pretreatment of D. officinale with organic solvents	1383:1433	CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.					
34775614	7	53	theme	polysaccharides	1537:1551	arg1	ratio					1528:1532	the mannose/glucose ratio	1508:1532	the mannose/glucose ratio of polysaccharides	1508:1551	CONCLUSION These results suggest that pretreatment of D. officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio of polysaccharides, which plays an important role in immunostimulation.					
34775614	0	54	from	officinale	106:115	arg1	pretreatments					124:136	the pretreatments	120:136	the pretreatments	120:136	Changes in the component sugar and immunostimulating activity of polysaccharides isolated from Dendrobium officinale in the pretreatments.					
34775614	6	55	theme	molecular	1278:1286	arg1	weight					1288:1293	a molecular weight	1276:1293	a molecular weight of 250 kDa	1276:1304	In addition, DOOS-CP was a somewhat refined fraction containing a major peak, representing a molecular weight of 250 kDa, despite being a crude polysaccharide.					
34775614	3	56	theme	macrophage	744:753	arg1	stimulation					755:765	macrophage stimulation	744:765	macrophage stimulation	744:765	RESULTS When their immunostimulatory activities were compared by macrophage stimulation and intestinal immune system modulation via Peyer's patches, DOOS-CP showed more potent activity than DO-CP.					
34775614	0	57	theme	polysaccharides	65:79	arg1	sugar					25:29	the component sugar and immunostimulating activity	11:60	sugar	25:29	Changes in the component sugar and immunostimulating activity of polysaccharides isolated from Dendrobium officinale in the pretreatments.					
34775614	0	57	theme	polysaccharides	65:79	arg1	activity					53:60	the component sugar and immunostimulating activity	11:60	activity	53:60	Changes in the component sugar and immunostimulating activity of polysaccharides isolated from Dendrobium officinale in the pretreatments.					
34775614	1	58	used	used	255:258	arg2	officinale					234:243	Dendrobium officinale	223:243	Dendrobium officinale	223:243	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	1	58	used	used	255:258	arg2	medicine					272:279	a herbal medicine	263:279	a herbal medicine in China and Southeast Asia, D. officinale	263:322	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	5	59	theme	composition	1076:1086	arg1	ratio					1088:1092	the composition ratio	1072:1092	the composition ratio of mannose and glucose	1072:1115	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	5	59	theme	composition	1076:1086	arg1	3.0					1121:1123	3.0	1121:1123	3.0	1121:1123	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	5	60	theme	lowest	1138:1143	arg1	2.0					1172:1174	2.0	1172:1174	2.0	1172:1174	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	5	60	theme	lowest	1138:1143	arg1	content					1160:1166	the lowest polysaccharide content	1134:1166	the lowest polysaccharide content	1134:1166	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	5	61	dep	contained	1031:1039	arg1	whereas					1126:1132	whereas	1126:1132	whereas	1126:1132	The most active polysaccharide contained 95% or more neutral sugar, and the composition ratio of mannose and glucose was 3.0, whereas the lowest polysaccharide content was 2.0 or less.					
34775614	1	62	theme	herbal	265:270	arg1	officinale					234:243	Dendrobium officinale	223:243	Dendrobium officinale	223:243	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
34775614	1	62	theme	herbal	265:270	arg1	medicine					272:279	a herbal medicine	263:279	a herbal medicine in China and Southeast Asia, D. officinale	263:322	BACKGROUND To isolate polysaccharides with enhanced immunostimulatory activity from Dendrobium officinale, which is used as a herbal medicine in China and Southeast Asia, D. officinale (DO) was pretreated with organic solvents (DOOS) or puffing at 7.5 and 9.0 kgf (7.5DO and 9DO).					
33183589	7	0	theme	BC/HA	1132:1136	arg1	composite					1138:1146	This chemically cross-linked BC/HA composite	1103:1146	This chemically cross-linked BC/HA composite	1103:1146	This chemically cross-linked BC/HA composite may have many new biomedical applications in wound care.					
33183589	5	1	theme	%	654:654	arg1	HA					656:657	2% HA	653:657	2% HA	653:657	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	6	2	theme	BC/HA	1031:1035	arg1	composite					1037:1045	the BDDE cross-linked BC/HA composite	1009:1045	the BDDE cross-linked BC/HA composite	1009:1045	In addition, cell toxicity assays and morphology indicated the BDDE cross-linked BC/HA composite significantly promoted cell proliferation and adhesion.					
33183589	0	3	theme	biological	95:104	arg1	applications					106:117	potential biological applications	85:117	potential biological applications	85:117	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.					
33183589	5	4	theme	thermal	846:852	arg1	°C					902:903	maximum degradation temperature: 360 °C	865:903	maximum degradation temperature: 360 °C	865:903	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	4	theme	thermal	846:852	arg1	stability					854:862	thermal stability	846:862	thermal stability (maximum degradation temperature: 360 °C)	846:904	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	4	5	theme	retention	566:574	arg1	capability					576:585	water retention capability	560:585	water retention capability	560:585	This dense morphology improved water retention capability and dimensional stability.					
33183589	5	6	theme	water	715:719	arg1	solubility					745:754	12.7 % water solubility	732:754	12.7 % water solubility	732:754	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	6	theme	water	715:719	arg1	stability					721:729	water stability	715:729	water stability (12.7 % water solubility)	715:755	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	7	dep	strength	789:796	arg1	MPa					837:839	∼1.62 MPa	831:839	tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa	781:839	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	0	8	link	cross-linked	13:24	arg1	hydrogel					72:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel	0:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.	0:118	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.					
33183589	1	9	theme	water	265:269	arg1	capability					279:288	water holding capability	265:288	water holding capability	265:288	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	7	10	theme	many	1157:1160	arg1	applications					1177:1188	many new biomedical applications	1157:1188	many new biomedical applications	1157:1188	This chemically cross-linked BC/HA composite may have many new biomedical applications in wound care.					
33183589	5	11	theme	1	663:663	arg1	%					664:664	%	664:664	%	664:664	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	12	theme	BDDE	614:617	arg1	BC/HA					632:636	BDDE cross-linked BC/HA composite	614:646	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE	614:669	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	7	13	contain	have	1152:1155	arg1	composite					1138:1146	This chemically cross-linked BC/HA composite	1103:1146	This chemically cross-linked BC/HA composite	1103:1146	This chemically cross-linked BC/HA composite may have many new biomedical applications in wound care.					
33183589	7	13	contain	have	1152:1155	arg2	applications					1177:1188	many new biomedical applications	1157:1188	many new biomedical applications	1157:1188	This chemically cross-linked BC/HA composite may have many new biomedical applications in wound care.					
33183589	1	14	theme	holding	271:277	arg1	capability					279:288	water holding capability	265:288	water holding capability	265:288	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	3	15	theme	BC/HA	471:475	arg1	composites					477:486	Cross-linked BC/HA composites	458:486	Cross-linked BC/HA composites	458:486	Cross-linked BC/HA composites exhibited a denser and smoother surface.					
33183589	5	16	dep	properties	769:778	arg1	strength					789:796	tensile strength	781:796	tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa	781:839	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	17	theme	tensile	781:787	arg1	strength					789:796	tensile strength	781:796	tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa	781:839	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	3	18	theme	smoother	511:518	arg1	surface					520:526	a denser and smoother surface	498:526	a denser and smoother surface	498:526	Cross-linked BC/HA composites exhibited a denser and smoother surface.					
33183589	1	19	theme	wound	178:182	arg1	dressing					184:191	wound dressing	178:191	wound dressing	178:191	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	6	20	theme	cell	963:966	arg1	assays					977:982	cell toxicity assays	963:982	cell toxicity assays	963:982	In addition, cell toxicity assays and morphology indicated the BDDE cross-linked BC/HA composite significantly promoted cell proliferation and adhesion.					
33183589	5	21	theme	composite	638:646	arg1	BC/HA					632:636	BDDE cross-linked BC/HA composite	614:646	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE	614:669	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	7	22	theme	new	1162:1164	arg1	applications					1177:1188	many new biomedical applications	1157:1188	many new biomedical applications	1157:1188	This chemically cross-linked BC/HA composite may have many new biomedical applications in wound care.					
33183589	2	23	theme	diglycidyl	391:400	arg1	BDDE					409:412	BDDE	409:412	BDDE	409:412	In this study, a composite hydrogel was prepared by using 1,4-butanediol diglycidyl ether (BDDE) to cross-link BC and hyaluronic acid (HA).					
33183589	2	23	theme	diglycidyl	391:400	arg1	ether					402:406	1,4-butanediol diglycidyl ether	376:406	1,4-butanediol diglycidyl ether (BDDE)	376:413	In this study, a composite hydrogel was prepared by using 1,4-butanediol diglycidyl ether (BDDE) to cross-link BC and hyaluronic acid (HA).					
33183589	2	24	theme	hyaluronic	436:445	arg1	HA					453:454	HA	453:454	HA	453:454	In this study, a composite hydrogel was prepared by using 1,4-butanediol diglycidyl ether (BDDE) to cross-link BC and hyaluronic acid (HA).					
33183589	2	24	theme	hyaluronic	436:445	arg1	acid					447:450	hyaluronic acid	436:450	hyaluronic acid (HA)	436:455	In this study, a composite hydrogel was prepared by using 1,4-butanediol diglycidyl ether (BDDE) to cross-link BC and hyaluronic acid (HA).					
33183589	6	25	theme	BDDE	1013:1016	arg1	composite					1037:1045	the BDDE cross-linked BC/HA composite	1009:1045	the BDDE cross-linked BC/HA composite	1009:1045	In addition, cell toxicity assays and morphology indicated the BDDE cross-linked BC/HA composite significantly promoted cell proliferation and adhesion.					
33183589	5	26	theme	%	737:737	arg1	solubility					745:754	12.7 % water solubility	732:754	12.7 % water solubility	732:754	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	26	theme	%	737:737	arg1	stability					721:729	water stability	715:729	water stability (12.7 % water solubility)	715:755	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	27	theme	temperature	885:895	arg1	°C					902:903	maximum degradation temperature: 360 °C	865:903	maximum degradation temperature: 360 °C	865:903	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	27	theme	temperature	885:895	arg1	stability					854:862	thermal stability	846:862	thermal stability (maximum degradation temperature: 360 °C)	846:904	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	3	28	theme	denser	500:505	arg1	surface					520:526	a denser and smoother surface	498:526	a denser and smoother surface	498:526	Cross-linked BC/HA composites exhibited a denser and smoother surface.					
33183589	2	29	theme	1,4-butanediol	376:389	arg1	BDDE					409:412	BDDE	409:412	BDDE	409:412	In this study, a composite hydrogel was prepared by using 1,4-butanediol diglycidyl ether (BDDE) to cross-link BC and hyaluronic acid (HA).					
33183589	2	29	theme	1,4-butanediol	376:389	arg1	ether					402:406	1,4-butanediol diglycidyl ether	376:406	1,4-butanediol diglycidyl ether (BDDE)	376:413	In this study, a composite hydrogel was prepared by using 1,4-butanediol diglycidyl ether (BDDE) to cross-link BC and hyaluronic acid (HA).					
33183589	5	30	theme	2	653:653	arg1	%					654:654	%	654:654	%	654:654	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	31	theme	cross-linked	619:630	arg1	BC/HA					632:636	BDDE cross-linked BC/HA composite	614:646	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE	614:669	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	1	32	theme	Bacterial	120:128	arg1	candidate					164:172	a good material candidate	148:172	a good material candidate for wound dressing	148:191	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	1	32	theme	Bacterial	120:128	arg1	BC					141:142	BC	141:142	BC	141:142	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	1	32	theme	Bacterial	120:128	arg1	cellulose					130:138	Bacterial cellulose	120:138	Bacterial cellulose (BC)	120:143	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	0	33	theme	hyaluronic	26:35	arg1	hydrogel					72:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel	0:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.	0:118	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.					
33183589	4	34	theme	dense	534:538	arg1	morphology					540:549	This dense morphology	529:549	This dense morphology	529:549	This dense morphology improved water retention capability and dimensional stability.					
33183589	5	35	theme	overall	685:691	arg1	properties					769:778	mechanical properties	758:778	mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa)	758:840	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	35	theme	overall	685:691	arg1	properties					693:702	better overall properties	678:702	better overall properties	678:702	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	35	theme	overall	685:691	arg1	stability					721:729	water stability	715:729	water stability (12.7 % water solubility)	715:755	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	35	theme	overall	685:691	arg1	stability					854:862	thermal stability	846:862	thermal stability (maximum degradation temperature: 360 °C)	846:904	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	36	theme	degradation	873:883	arg1	°C					902:903	maximum degradation temperature: 360 °C	865:903	maximum degradation temperature: 360 °C	865:903	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	36	theme	degradation	873:883	arg1	stability					854:862	thermal stability	846:862	thermal stability (maximum degradation temperature: 360 °C)	846:904	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	0	37	theme	cross-linked	13:24	arg1	hydrogel					72:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel	0:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.	0:118	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.					
33183589	4	38	theme	water	560:564	arg1	capability					576:585	water retention capability	560:585	water retention capability	560:585	This dense morphology improved water retention capability and dimensional stability.					
33183589	6	39	theme	cross-linked	1018:1029	arg1	composite					1037:1045	the BDDE cross-linked BC/HA composite	1009:1045	the BDDE cross-linked BC/HA composite	1009:1045	In addition, cell toxicity assays and morphology indicated the BDDE cross-linked BC/HA composite significantly promoted cell proliferation and adhesion.					
33183589	1	40	theme	fine	208:211	arg1	structure					225:233	its fine 3-D network structure	204:233	its fine 3-D network structure	204:233	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	0	41	theme	cellulose	52:60	arg1	hydrogel					72:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel	0:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.	0:118	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.					
33183589	1	42	theme	good	295:298	arg1	biocompatibility					300:315	good biocompatibility	295:315	good biocompatibility	295:315	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	6	43	theme	toxicity	968:975	arg1	assays					977:982	cell toxicity assays	963:982	cell toxicity assays	963:982	In addition, cell toxicity assays and morphology indicated the BDDE cross-linked BC/HA composite significantly promoted cell proliferation and adhesion.					
33183589	1	44	theme	3-D	213:215	arg1	structure					225:233	its fine 3-D network structure	204:233	its fine 3-D network structure	204:233	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	0	45	theme	acid/bacterial	37:50	arg1	hydrogel					72:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel	0:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.	0:118	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.					
33183589	5	46	link	cross-linked	619:630	arg1	BC/HA					632:636	BDDE cross-linked BC/HA composite	614:646	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE	614:669	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	7	47	theme	cross-linked	1119:1130	arg1	composite					1138:1146	This chemically cross-linked BC/HA composite	1103:1146	This chemically cross-linked BC/HA composite	1103:1146	This chemically cross-linked BC/HA composite may have many new biomedical applications in wound care.					
33183589	1	48	theme	network	217:223	arg1	structure					225:233	its fine 3-D network structure	204:233	its fine 3-D network structure	204:233	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	7	49	link	cross-linked	1119:1130	arg1	composite					1138:1146	This chemically cross-linked BC/HA composite	1103:1146	This chemically cross-linked BC/HA composite	1103:1146	This chemically cross-linked BC/HA composite may have many new biomedical applications in wound care.					
33183589	5	50	theme	%	664:664	arg1	BDDE					666:669	1% BDDE	663:669	1% BDDE	663:669	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	51	theme	water	739:743	arg1	solubility					745:754	12.7 % water solubility	732:754	12.7 % water solubility	732:754	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	51	theme	water	739:743	arg1	stability					721:729	water stability	715:729	water stability (12.7 % water solubility)	715:755	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	6	52	theme	cell	1070:1073	arg1	proliferation					1075:1087	cell proliferation	1070:1087	cell proliferation	1070:1087	In addition, cell toxicity assays and morphology indicated the BDDE cross-linked BC/HA composite significantly promoted cell proliferation and adhesion.					
33183589	2	53	theme	composite	335:343	arg1	hydrogel					345:352	a composite hydrogel	333:352	a composite hydrogel	333:352	In this study, a composite hydrogel was prepared by using 1,4-butanediol diglycidyl ether (BDDE) to cross-link BC and hyaluronic acid (HA).					
33183589	0	54	theme	composite	62:70	arg1	hydrogel					72:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel	0:79	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.	0:118	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.					
33183589	3	55	link	Cross-linked	458:469	arg1	composites					477:486	Cross-linked BC/HA composites	458:486	Cross-linked BC/HA composites	458:486	Cross-linked BC/HA composites exhibited a denser and smoother surface.					
33183589	7	56	theme	biomedical	1166:1175	arg1	applications					1177:1188	many new biomedical applications	1157:1188	many new biomedical applications	1157:1188	This chemically cross-linked BC/HA composite may have many new biomedical applications in wound care.					
33183589	7	57	theme	wound	1193:1197	arg1	care					1199:1202	wound care	1193:1202	wound care	1193:1202	This chemically cross-linked BC/HA composite may have many new biomedical applications in wound care.					
33183589	6	58	link	cross-linked	1018:1029	arg1	composite					1037:1045	the BDDE cross-linked BC/HA composite	1009:1045	the BDDE cross-linked BC/HA composite	1009:1045	In addition, cell toxicity assays and morphology indicated the BDDE cross-linked BC/HA composite significantly promoted cell proliferation and adhesion.					
33183589	5	59	theme	mechanical	758:767	arg1	properties					769:778	mechanical properties	758:778	mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa)	758:840	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	60	theme	maximum	865:871	arg1	°C					902:903	maximum degradation temperature: 360 °C	865:903	maximum degradation temperature: 360 °C	865:903	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	60	theme	maximum	865:871	arg1	stability					854:862	thermal stability	846:862	thermal stability (maximum degradation temperature: 360 °C)	846:904	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	61	theme	better	678:683	arg1	properties					769:778	mechanical properties	758:778	mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa)	758:840	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	61	theme	better	678:683	arg1	properties					693:702	better overall properties	678:702	better overall properties	678:702	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	61	theme	better	678:683	arg1	stability					721:729	water stability	715:729	water stability (12.7 % water solubility)	715:755	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	61	theme	better	678:683	arg1	stability					854:862	thermal stability	846:862	thermal stability (maximum degradation temperature: 360 °C)	846:904	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	3	62	theme	Cross-linked	458:469	arg1	composites					477:486	Cross-linked BC/HA composites	458:486	Cross-linked BC/HA composites	458:486	Cross-linked BC/HA composites exhibited a denser and smoother surface.					
33183589	5	63	with	BC/HA	632:636	arg1	HA					656:657	2% HA	653:657	2% HA	653:657	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	5	63	with	BC/HA	632:636	arg1	BDDE					666:669	1% BDDE	663:669	1% BDDE	663:669	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	0	64	theme	potential	85:93	arg1	applications					106:117	potential biological applications	85:117	potential biological applications	85:117	A covalently cross-linked hyaluronic acid/bacterial cellulose composite hydrogel for potential biological applications.					
33183589	5	65	theme	Young	814:818	arg1	modulus					822:828	Young's modulus	814:828	Young's modulus	814:828	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	1	66	theme	good	150:153	arg1	cellulose					130:138	Bacterial cellulose	120:138	Bacterial cellulose (BC)	120:143	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	1	66	theme	good	150:153	arg1	candidate					164:172	a good material candidate	148:172	a good material candidate for wound dressing	148:191	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	1	67	theme	high	236:239	arg1	strength					252:259	high mechanical strength	236:259	high mechanical strength	236:259	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	4	68	theme	dimensional	591:601	arg1	stability					603:611	dimensional stability	591:611	dimensional stability	591:611	This dense morphology improved water retention capability and dimensional stability.					
33183589	5	69	theme	∼	799:799	arg1	MPa					806:808	∼ 0.61 MPa	799:808	∼ 0.61 MPa	799:808	BDDE cross-linked BC/HA composite with 2% HA and 1% BDDE showed better overall properties, including water stability (12.7 % water solubility), mechanical properties (tensile strength: ∼ 0.61 MPa and Young's modulus: ∼1.62 MPa) and thermal stability (maximum degradation temperature: 360 °C), as compared to BC/HA without crosslinking.					
33183589	1	70	theme	material	155:162	arg1	cellulose					130:138	Bacterial cellulose	120:138	Bacterial cellulose (BC)	120:143	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	1	70	theme	material	155:162	arg1	candidate					164:172	a good material candidate	148:172	a good material candidate for wound dressing	148:191	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
33183589	1	71	theme	mechanical	241:250	arg1	strength					252:259	high mechanical strength	236:259	high mechanical strength	236:259	Bacterial cellulose (BC) is a good material candidate for wound dressing because of its fine 3-D network structure, high mechanical strength and water holding capability, and good biocompatibility.					
32252314	6	0	theme	chitosan	1304:1311	arg1	gelation					1292:1299	ionotropic gelation	1281:1299	ionotropic gelation of chitosan	1281:1311	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	1	dep	used	969:972	arg1	included					974:981	included	974:981	included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions	974:1196	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	1	dep	used	969:972	arg1	Methods					961:967	Methods	961:967	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.	961:1431	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	5	2	from	post-treatment	837:850	arg1	solution					882:889	phosphotungstic acid (PWA) solution	855:889	phosphotungstic acid (PWA) solution	855:889	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	1	3	theme	eco-friendly	155:166	arg1	Chit					189:192	Chit	189:192	Chit	189:192	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	1	3	theme	eco-friendly	155:166	arg1	chitosan					179:186	biopolymer chitosan	168:186	cost-effective and eco-friendly biopolymer chitosan (Chit)	136:193	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	4	4	from	gelation	685:692	arg1	substrate					716:724	an alumina substrate	705:724	an alumina substrate	705:724	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	1	5	theme	one-step	236:243	arg1	synthesis					245:253	the one-step synthesis	232:253	the one-step synthesis of proton-conducting fuel cell bio-membranes	232:298	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	6	6	dep	structural	992:1001	arg1	XRD					1004:1006	XRD	1004:1006	XRD	1004:1006	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	6	dep	structural	992:1001	arg1	thermal-gravimetric					1010:1028	thermal-gravimetric	1010:1028	thermal-gravimetric	1010:1028	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	6	dep	structural	992:1001	arg1	compositional					1068:1080	compositional	1068:1080	compositional	1068:1080	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	6	dep	structural	992:1001	arg1	electrochemical					1037:1051	electrochemical	1037:1051	electrochemical	1037:1051	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	1	7	theme	biopolymer	168:177	arg1	Chit					189:192	Chit	189:192	Chit	189:192	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	1	7	theme	biopolymer	168:177	arg1	chitosan					179:186	biopolymer chitosan	168:186	cost-effective and eco-friendly biopolymer chitosan (Chit)	136:193	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	6	8	theme	conductive	1382:1391	arg1	membranes					1407:1415	novel proton conductive bio-composite membranes	1369:1415	novel proton conductive bio-composite membranes	1369:1415	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	9	theme	combined	983:990	arg1	SEM					1112:1114	SEM	1112:1114	SEM	1112:1114	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	9	theme	combined	983:990	arg1	analysis					1102:1109	combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis	983:1109	combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM)	983:1115	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	10	theme	gelation	1292:1299	arg1	impact					1271:1276	the impact	1267:1276	the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures	1267:1430	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	11	dep	electrochemical	1037:1051	arg1	EIS					1062:1064	in-situ EIS	1054:1064	in-situ EIS	1054:1064	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	4	12	dep	Methods	628:634	arg1	analyzed					778:785	analyzed	778:785	analyzed	778:785	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	4	12	dep	Methods	628:634	arg1	discussed					764:772	discussed	764:772	discussed	764:772	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	4	12	dep	Methods	628:634	arg1	2					625:625	2	625:625	2	625:625	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	3	13	theme	topic	537:541	arg1	aspects					521:527	the key aspects	513:527	the key aspects of this topic	513:541	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	4	14	theme	phosphotungtate	729:743	arg1	PWA3-					753:757	PWA3-	753:757	PWA3-	753:757	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	4	14	theme	phosphotungtate	729:743	arg1	anions					745:750	phosphotungtate anions	729:750	phosphotungtate anions (PWA3-)	729:758	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	6	15	theme	ionotropic	1281:1290	arg1	gelation					1292:1299	ionotropic gelation	1281:1299	ionotropic gelation of chitosan	1281:1311	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	16	dep	thermal-gravimetric	1010:1028	arg1	DTG					1031:1033	DTG	1031:1033	DTG	1031:1033	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	1	17	theme	proton-conducting	258:274	arg1	bio-membranes					286:298	proton-conducting fuel cell bio-membranes	258:298	proton-conducting fuel cell bio-membranes	258:298	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	6	18	from	impact	1271:1276	arg1	working					1341:1347	working	1341:1347	working	1341:1347	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	18	from	impact	1271:1276	arg1	community					1331:1339	the scientific community	1316:1339	the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures	1316:1430	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	19	theme	bio-composite	1393:1405	arg1	membranes					1407:1415	novel proton conductive bio-composite membranes	1369:1415	novel proton conductive bio-composite membranes	1369:1415	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	4	20	from	mechanism	653:661	arg1	substrate					716:724	an alumina substrate	705:724	an alumina substrate	705:724	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	1	21	theme	chitosan	179:186	arg1	gelation					124:131	Ionotropic gelation	113:131	Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit)	113:193	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	1	21	theme	chitosan	179:186	arg1	approach					220:227	a novel and promising approach	198:227	a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes	198:298	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	5	22	from	solution	882:889	arg1	effect					817:822	the effect	813:822	the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	813:958	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	6	23	from	performances	1133:1144	arg1	Conclusions					1186:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	24	theme	structural	992:1001	arg1	SEM					1112:1114	SEM	1112:1114	SEM	1112:1114	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	24	theme	structural	992:1001	arg1	analysis					1102:1109	combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis	983:1109	combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM)	983:1115	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	5	25	theme	post-treatment	837:850	arg1	effect					817:822	the effect	813:822	the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	813:958	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	6	26	theme	novel	1369:1373	arg1	membranes					1407:1415	novel proton conductive bio-composite membranes	1369:1415	novel proton conductive bio-composite membranes	1369:1415	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	27	theme	cell	1178:1181	arg1	Conclusions					1186:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	5	28	theme	chitosan/phosphotungstate	907:931	arg1	structures					949:958	the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	894:958	the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	894:958	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	2	29	theme	few	380:382	arg1	papers					384:389	very few papers	375:389	very few papers	375:389	The method discovered by the author in 2011 and subsequently drowned among very few papers.					
32252314	0	30	theme	Ionotropic	0:9	arg1	Gelation					11:18	Ionotropic Gelation	0:18	Ionotropic Gelation of Chitosan for Next-Generation Composite Proton Conducting Flat Structures.	0:95	Ionotropic Gelation of Chitosan for Next-Generation Composite Proton Conducting Flat Structures.					
32252314	1	31	theme	fuel	276:279	arg1	bio-membranes					286:298	proton-conducting fuel cell bio-membranes	258:298	proton-conducting fuel cell bio-membranes	258:298	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	6	32	theme	fuel	1173:1176	arg1	Conclusions					1186:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	5	33	theme	prolonged	827:835	arg1	post-treatment					837:850	prolonged post-treatment	827:850	prolonged post-treatment in phosphotungstic acid (PWA) solution	827:889	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	6	34	from	analysis	1102:1109	arg1	Conclusions					1186:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	5	35	theme	phosphotungstic	855:869	arg1	PWA					877:879	PWA	877:879	PWA	877:879	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	5	35	theme	phosphotungstic	855:869	arg1	acid					871:874	phosphotungstic acid	855:874	phosphotungstic acid (PWA) solution	855:889	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	0	36	theme	Chitosan	23:30	arg1	Gelation					11:18	Ionotropic Gelation	0:18	Ionotropic Gelation of Chitosan for Next-Generation Composite Proton Conducting Flat Structures.	0:95	Ionotropic Gelation of Chitosan for Next-Generation Composite Proton Conducting Flat Structures.					
32252314	1	37	theme	cell	281:284	arg1	bio-membranes					286:298	proton-conducting fuel cell bio-membranes	258:298	proton-conducting fuel cell bio-membranes	258:298	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	4	38	from	substrate	716:724	arg1	mechanism					653:661	The mechanism	649:661	The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-)	649:758	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	3	39	theme	successful	547:556	arg1	dissemination					558:570	successful dissemination	547:570	successful dissemination of the results and significant future developments	547:621	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	6	40	theme	H2/O2	1167:1171	arg1	Conclusions					1186:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	3	41	theme	effective	450:458	arg1	communication					460:472	clear and effective communication	440:472	clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments	440:621	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	1	42	theme	bio-membranes	286:298	arg1	synthesis					245:253	the one-step synthesis	232:253	the one-step synthesis of proton-conducting fuel cell bio-membranes	232:298	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	6	43	theme	scientific	1320:1329	arg1	working					1341:1347	working	1341:1347	working	1341:1347	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	43	theme	scientific	1320:1329	arg1	community					1331:1339	the scientific community	1316:1339	the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures	1316:1430	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	4	44	theme	gelation	685:692	arg1	mechanism					653:661	The mechanism	649:661	The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-)	649:758	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	3	45	theme	key	517:519	arg1	aspects					521:527	the key aspects	513:527	the key aspects of this topic	513:541	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	0	46	theme	Next-Generation	36:50	arg1	Structures					85:94	Next-Generation Composite Proton Conducting Flat Structures	36:94	Next-Generation Composite Proton Conducting Flat Structures	36:94	Ionotropic Gelation of Chitosan for Next-Generation Composite Proton Conducting Flat Structures.					
32252314	3	47	theme	results	493:499	arg1	communication					460:472	clear and effective communication	440:472	clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments	440:621	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	6	48	theme	low	1151:1153	arg1	Conclusions					1186:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	3	49	theme	clear	440:444	arg1	communication					460:472	clear and effective communication	440:472	clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments	440:621	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	3	50	theme	significant	591:601	arg1	developments					610:621	significant future developments	591:621	significant future developments	591:621	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	1	51	theme	novel	200:204	arg1	gelation					124:131	Ionotropic gelation	113:131	Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit)	113:193	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	1	51	theme	novel	200:204	arg1	approach					220:227	a novel and promising approach	198:227	a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes	198:298	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	4	52	theme	ionotropic	674:683	arg1	gelation					685:692	in-situ ionotropic gelation	666:692	in-situ ionotropic gelation of Chit on an alumina substrate	666:724	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	3	53	theme	future	603:608	arg1	developments					610:621	significant future developments	591:621	significant future developments	591:621	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	5	54	from	effect	817:822	arg1	structures					949:958	the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	894:958	the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	894:958	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	5	54	from	effect	817:822	arg1	solution					882:889	phosphotungstic acid (PWA) solution	855:889	phosphotungstic acid (PWA) solution	855:889	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	0	55	theme	Proton	62:67	arg1	Structures					85:94	Next-Generation Composite Proton Conducting Flat Structures	36:94	Next-Generation Composite Proton Conducting Flat Structures	36:94	Ionotropic Gelation of Chitosan for Next-Generation Composite Proton Conducting Flat Structures.					
32252314	5	56	theme	acid	871:874	arg1	solution					882:889	phosphotungstic acid (PWA) solution	855:889	phosphotungstic acid (PWA) solution	855:889	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	3	57	theme	results	579:585	arg1	dissemination					558:570	successful dissemination	547:570	successful dissemination of the results and significant future developments	547:621	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	3	58	theme	unpublished	481:491	arg1	results					493:499	new unpublished results	477:499	new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments	477:621	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	0	59	theme	Composite	52:60	arg1	Structures					85:94	Next-Generation Composite Proton Conducting Flat Structures	36:94	Next-Generation Composite Proton Conducting Flat Structures	36:94	Ionotropic Gelation of Chitosan for Next-Generation Composite Proton Conducting Flat Structures.					
32252314	6	60	theme	temperature	1155:1165	arg1	Conclusions					1186:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	a low temperature H2/O2 fuel cell(4) Conclusions	1149:1196	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	61	theme	novel	1227:1231	arg1	possibilities					1233:1245	novel possibilities	1227:1245	novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures	1227:1430	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	62	from	community	1331:1339	arg1	synthesis					1356:1364	the synthesis	1352:1364	the synthesis of novel proton conductive bio-composite membranes and structures	1352:1430	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	1	63	theme	Ionotropic	113:122	arg1	gelation					124:131	Ionotropic gelation	113:131	Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit)	113:193	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	1	63	theme	Ionotropic	113:122	arg1	approach					220:227	a novel and promising approach	198:227	a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes	198:298	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	4	64	theme	in-situ	666:672	arg1	gelation					685:692	in-situ ionotropic gelation	666:692	in-situ ionotropic gelation of Chit on an alumina substrate	666:724	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	4	65	theme	alumina	708:714	arg1	substrate					716:724	an alumina substrate	705:724	an alumina substrate	705:724	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
32252314	5	66	theme	Chit-PWA	934:941	arg1	structures					949:958	the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	894:958	the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	894:958	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	1	67	theme	promising	210:218	arg1	gelation					124:131	Ionotropic gelation	113:131	Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit)	113:193	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	1	67	theme	promising	210:218	arg1	approach					220:227	a novel and promising approach	198:227	a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes	198:298	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	6	68	dep	Methods	961:967	arg1	discloses					1217:1225	discloses	1217:1225	discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures	1217:1430	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	5	69	theme	flat	944:947	arg1	structures					949:958	the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	894:958	the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	894:958	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	3	70	theme	new	477:479	arg1	results					493:499	new unpublished results	477:499	new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments	477:621	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	6	71	theme	structures	1421:1430	arg1	synthesis					1356:1364	the synthesis	1352:1364	the synthesis of novel proton conductive bio-composite membranes and structures	1352:1430	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	5	72	theme	obtained	898:905	arg1	structures					949:958	the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	894:958	the obtained chitosan/phosphotungstate (Chit-PWA) flat structures	894:958	The study sheds light on the effect of prolonged post-treatment in phosphotungstic acid (PWA) solution on the obtained chitosan/phosphotungstate (Chit-PWA) flat structures.					
32252314	0	73	theme	Flat	80:83	arg1	Structures					85:94	Next-Generation Composite Proton Conducting Flat Structures	36:94	Next-Generation Composite Proton Conducting Flat Structures	36:94	Ionotropic Gelation of Chitosan for Next-Generation Composite Proton Conducting Flat Structures.					
32252314	6	74	theme	proton	1375:1380	arg1	membranes					1407:1415	novel proton conductive bio-composite membranes	1369:1415	novel proton conductive bio-composite membranes	1369:1415	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	3	75	theme	developments	610:621	arg1	dissemination					558:570	successful dissemination	547:570	successful dissemination of the results and significant future developments	547:621	This work aimed to relaunch this method through clear and effective communication of new unpublished results emphasizing the key aspects of this topic for successful dissemination of the results and significant future developments.					
32252314	1	76	theme	cost-effective	136:149	arg1	Chit					189:192	Chit	189:192	Chit	189:192	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	1	76	theme	cost-effective	136:149	arg1	chitosan					179:186	biopolymer chitosan	168:186	cost-effective and eco-friendly biopolymer chitosan (Chit)	136:193	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	0	77	theme	Conducting	69:78	arg1	Structures					85:94	Next-Generation Composite Proton Conducting Flat Structures	36:94	Next-Generation Composite Proton Conducting Flat Structures	36:94	Ionotropic Gelation of Chitosan for Next-Generation Composite Proton Conducting Flat Structures.					
32252314	6	78	theme	morphological	1088:1100	arg1	SEM					1112:1114	SEM	1112:1114	SEM	1112:1114	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	78	theme	morphological	1088:1100	arg1	analysis					1102:1109	combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis	983:1109	combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM)	983:1115	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	6	79	dep	compositional	1068:1080	arg1	EDX					1083:1085	EDX	1083:1085	EDX	1083:1085	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	1	80	dep	Background	101:110	arg1	gelation					124:131	Ionotropic gelation	113:131	Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit)	113:193	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	1	80	dep	Background	101:110	arg1	1					98:98	1	98:98	1	98:98	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	1	80	dep	Background	101:110	arg1	approach					220:227	a novel and promising approach	198:227	a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes	198:298	(1) Background: Ionotropic gelation of cost-effective and eco-friendly biopolymer chitosan (Chit) is a novel and promising approach to the one-step synthesis of proton-conducting fuel cell bio-membranes.					
32252314	6	81	theme	membranes	1407:1415	arg1	synthesis					1356:1364	the synthesis	1352:1364	the synthesis of novel proton conductive bio-composite membranes and structures	1352:1430	Methods used included combined structural (XRD), thermal-gravimetric (DTG), electrochemical (in-situ EIS), compositional (EDX),morphological analysis (SEM), as well as the performances in a low temperature H2/O2 fuel cell(4) Conclusions: This contribution discloses novel possibilities aimed at increasing the impact of ionotropic gelation of chitosan on the scientific community working on the synthesis of novel proton conductive bio-composite membranes and structures.					
32252314	4	82	theme	Chit	697:700	arg1	gelation					685:692	in-situ ionotropic gelation	666:692	in-situ ionotropic gelation of Chit on an alumina substrate	666:724	(2) Methods and results: The mechanism of in-situ ionotropic gelation of Chit on an alumina substrate by phosphotungtate anions (PWA3-) was discussed and analyzed.					
34135417	0	0	theme	functional	94:103	arg1	ingredients					110:120	nutritionally-rich functional food ingredients	75:120	nutritionally-rich functional food ingredients	75:120	The composition of Australian Plantago seeds highlights their potential as nutritionally-rich functional food ingredients.					
34135417	3	1	theme	analyses	686:693	arg1	use					628:630	the use	624:630	the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options	624:869	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	4	2	theme	interspecific	897:909	arg1	differences					911:921	substantial interspecific differences	885:921	substantial interspecific differences in mucilage yield and macromolecular properties	885:969	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	4	2	theme	interspecific	897:909	arg1	consequence					984:994	a consequence	982:994	a consequence of differences in heteroxylan and pectin composition	982:1047	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	0	3	theme	nutritionally-rich	75:92	arg1	ingredients					110:120	nutritionally-rich functional food ingredients	75:120	nutritionally-rich functional food ingredients	75:120	The composition of Australian Plantago seeds highlights their potential as nutritionally-rich functional food ingredients.					
34135417	6	4	theme	Plantago	1317:1324	arg1	flour					1326:1330	Whole seed Plantago flour	1306:1330	Whole seed Plantago flour	1306:1330	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	4	5	from	differences	1077:1087	arg1	functionality					1105:1117	hydrocolloid functionality	1092:1117	hydrocolloid functionality	1092:1117	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	5	6	theme	non-mucilage	1172:1183	arg1	tissues					1206:1212	non-mucilage producing inner seed tissues	1172:1212	non-mucilage producing inner seed tissues	1172:1212	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	6	7	theme	seed	1312:1315	arg1	flour					1326:1330	Whole seed Plantago flour	1306:1330	Whole seed Plantago flour	1306:1330	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	2	8	theme	dry	292:294	arg1	layer					301:305	the dry husk layer	288:305	the dry husk layer that becomes mucilage, called psyllium,	288:345	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	2	8	theme	dry	292:294	arg1	milled					350:355	milled	350:355	milled	350:355	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	4	9	theme	hydrocolloid	1092:1103	arg1	functionality					1105:1117	hydrocolloid functionality	1092:1117	hydrocolloid functionality	1092:1117	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	1	10	theme	fibre	267:271	arg1	value					222:226	value	222:226	value	222:226	When wetted, Plantago seeds become covered with a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre.					
34135417	1	10	theme	fibre	267:271	arg1	food					233:236	a food	231:236	a food additive and bulking dietary fibre	231:271	When wetted, Plantago seeds become covered with a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre.					
34135417	0	11	theme	food	105:108	arg1	ingredients					110:120	nutritionally-rich functional food ingredients	75:120	nutritionally-rich functional food ingredients	75:120	The composition of Australian Plantago seeds highlights their potential as nutritionally-rich functional food ingredients.					
34135417	3	12	theme	whole	706:710	arg1	composition/characteristics					717:743	whole seed composition/characteristics	706:743	whole seed composition/characteristics of P. ovata	706:755	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	4	13	from	consequence	984:994	arg1	heteroxylan					1014:1024	heteroxylan	1014:1024	heteroxylan	1014:1024	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	4	13	from	consequence	984:994	arg1	composition					1037:1047	pectin composition	1030:1047	pectin composition	1030:1047	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	6	14	theme	Whole	1306:1310	arg1	flour					1326:1330	Whole seed Plantago flour	1306:1330	Whole seed Plantago flour	1306:1330	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	6	15	theme	harsh	1378:1382	arg1	environments					1395:1406	harsh Australian environments	1378:1406	harsh Australian environments	1378:1406	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	2	16	theme	value	492:496	arg1	feed					505:508	low value animal feed	488:508	low value animal feed	488:508	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	3	17	theme	seed	712:715	arg1	composition/characteristics					717:743	whole seed composition/characteristics	706:743	whole seed composition/characteristics of P. ovata	706:755	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	6	18	theme	P.	1480:1481	arg1	husk					1498:1501	purified P. ovata psyllium husk	1471:1501	purified P. ovata psyllium husk	1471:1501	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	1	19	theme	polysaccharide-rich	173:191	arg1	gel					193:195	a polysaccharide-rich gel	171:195	a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre	171:271	When wetted, Plantago seeds become covered with a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre.					
34135417	2	20	theme	low	488:490	arg1	feed					505:508	low value animal feed	488:508	low value animal feed	488:508	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	6	21	theme	Australian	1384:1393	arg1	environments					1395:1406	harsh Australian environments	1378:1406	harsh Australian environments	1378:1406	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	4	22	from	differences	999:1009	arg1	heteroxylan					1014:1024	heteroxylan	1014:1024	heteroxylan	1014:1024	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	4	22	from	differences	999:1009	arg1	composition					1037:1047	pectin composition	1030:1047	pectin composition	1030:1047	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	6	23	theme	essential	1581:1589	arg1	nutrients					1591:1599	additional essential nutrients	1570:1599	additional essential nutrients	1570:1599	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	6	24	theme	psyllium	1489:1496	arg1	husk					1498:1501	purified P. ovata psyllium husk	1471:1501	purified P. ovata psyllium husk	1471:1501	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	4	25	theme	differences	999:1009	arg1	differences					911:921	substantial interspecific differences	885:921	substantial interspecific differences in mucilage yield and macromolecular properties	885:969	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	4	25	theme	differences	999:1009	arg1	consequence					984:994	a consequence	982:994	a consequence of differences in heteroxylan and pectin composition	982:1047	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	6	26	theme	additional	1570:1579	arg1	nutrients					1591:1599	additional essential nutrients	1570:1599	additional essential nutrients	1570:1599	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	2	27	theme	ovata	370:374	arg1	seeds					376:380	Plantago ovata seeds	361:380	Plantago ovata seeds	361:380	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	2	27	theme	ovata	370:374	arg1	species					421:427	the only commercial-relevant Plantago species	383:427	the only commercial-relevant Plantago species	383:427	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	2	28	theme	Plantago	361:368	arg1	seeds					376:380	Plantago ovata seeds	361:380	Plantago ovata seeds	361:380	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	2	28	theme	Plantago	361:368	arg1	species					421:427	the only commercial-relevant Plantago species	383:427	the only commercial-relevant Plantago species	383:427	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	0	29	theme	Australian	19:28	arg1	seeds					39:43	Australian Plantago seeds	19:43	Australian Plantago seeds	19:43	The composition of Australian Plantago seeds highlights their potential as nutritionally-rich functional food ingredients.					
34135417	3	30	theme	ovata	751:755	arg1	composition/characteristics					717:743	whole seed composition/characteristics	706:743	whole seed composition/characteristics of P. ovata	706:755	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	5	31	from	high	1259:1262	arg1	sugars					1279:1284	fermentable sugars	1267:1284	fermentable sugars	1267:1284	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	2	32	theme	Plantago	412:419	arg1	seeds					376:380	Plantago ovata seeds	361:380	Plantago ovata seeds	361:380	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	2	32	theme	Plantago	412:419	arg1	species					421:427	the only commercial-relevant Plantago species	383:427	the only commercial-relevant Plantago species	383:427	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	6	33	theme	purified	1471:1478	arg1	husk					1498:1501	purified P. ovata psyllium husk	1471:1501	purified P. ovata psyllium husk	1471:1501	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	5	34	theme	substantial	1224:1234	arg1	endosperm					1248:1256	a substantial mannan-rich endosperm	1222:1256	a substantial mannan-rich endosperm	1222:1256	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	4	35	theme	mucilage	926:933	arg1	yield					935:939	mucilage yield	926:939	mucilage yield	926:939	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	2	36	theme	husk	296:299	arg1	layer					301:305	the dry husk layer	288:305	the dry husk layer that becomes mucilage, called psyllium,	288:345	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	2	36	theme	husk	296:299	arg1	milled					350:355	milled	350:355	milled	350:355	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	6	37	theme	mucilage	1547:1554	arg1	functionality					1521:1533	the functionality	1517:1533	the functionality of the seed mucilage	1517:1554	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	2	38	theme	commercial-relevant	392:410	arg1	seeds					376:380	Plantago ovata seeds	361:380	Plantago ovata seeds	361:380	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	2	38	theme	commercial-relevant	392:410	arg1	species					421:427	the only commercial-relevant Plantago species	383:427	the only commercial-relevant Plantago species	383:427	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	0	39	theme	seeds	39:43	arg1	composition					4:14	The composition	0:14	The composition of Australian Plantago seeds	0:43	The composition of Australian Plantago seeds highlights their potential as nutritionally-rich functional food ingredients.					
34135417	5	40	theme	mannan-rich	1236:1246	arg1	endosperm					1248:1256	a substantial mannan-rich endosperm	1222:1256	a substantial mannan-rich endosperm	1222:1256	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	4	41	theme	macromolecular	945:958	arg1	properties					960:969	macromolecular properties	945:969	macromolecular properties	945:969	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	6	42	dep	P.	1480:1481	arg1	ovata					1483:1487	ovata	1483:1487	ovata	1483:1487	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	6	43	theme	seed	1542:1545	arg1	mucilage					1547:1554	the seed mucilage	1538:1554	the seed mucilage	1538:1554	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	0	44	theme	Plantago	30:37	arg1	seeds					39:43	Australian Plantago seeds	19:43	Australian Plantago seeds	19:43	The composition of Australian Plantago seeds highlights their potential as nutritionally-rich functional food ingredients.					
34135417	4	45	theme	pectin	1030:1035	arg1	composition					1037:1047	pectin composition	1030:1047	pectin composition	1030:1047	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	2	46	theme	seed	455:458	arg1	either					472:477	either	472:477	either	472:477	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	2	46	theme	seed	455:458	arg1	tissues					460:466	the residual inner seed tissues	436:466	the residual inner seed tissues	436:466	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	4	47	theme	wide	1072:1075	arg1	differences					1077:1087	wide differences	1072:1087	wide differences in hydrocolloid functionality that can be exploited in industry	1072:1151	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	5	48	contain	contain	1214:1220	arg2	protein					1287:1293	protein	1287:1293	protein	1287:1293	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	5	48	contain	contain	1214:1220	arg2	endosperm					1248:1256	a substantial mannan-rich endosperm	1222:1256	a substantial mannan-rich endosperm	1222:1256	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	5	48	contain	contain	1214:1220	arg1	tissues					1206:1212	non-mucilage producing inner seed tissues	1172:1212	non-mucilage producing inner seed tissues	1172:1212	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	5	48	contain	contain	1214:1220	arg2	fats					1300:1303	fats	1300:1303	fats	1300:1303	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	4	49	from	heteroxylan	1014:1024	arg1	differences					911:921	substantial interspecific differences	885:921	substantial interspecific differences in mucilage yield and macromolecular properties	885:969	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	4	49	from	heteroxylan	1014:1024	arg1	consequence					984:994	a consequence	982:994	a consequence of differences in heteroxylan and pectin composition	982:1047	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	2	50	theme	inner	449:453	arg1	either					472:477	either	472:477	either	472:477	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	2	50	theme	inner	449:453	arg1	tissues					460:466	the residual inner seed tissues	436:466	the residual inner seed tissues	436:466	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	4	51	from	composition	1037:1047	arg1	differences					911:921	substantial interspecific differences	885:921	substantial interspecific differences in mucilage yield and macromolecular properties	885:969	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	4	51	from	composition	1037:1047	arg1	consequence					984:994	a consequence	982:994	a consequence of differences in heteroxylan and pectin composition	982:1047	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	2	52	dep	either	472:477	arg1	used					479:482	used	479:482	used for low value animal feed	479:508	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	6	53	from	species	1356:1362	arg1	flour					1326:1330	Whole seed Plantago flour	1306:1330	Whole seed Plantago flour	1306:1330	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	2	54	theme	residual	440:447	arg1	either					472:477	either	472:477	either	472:477	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	2	54	theme	residual	440:447	arg1	tissues					460:466	the residual inner seed tissues	436:466	the residual inner seed tissues	436:466	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	5	55	theme	seed	1201:1204	arg1	tissues					1206:1212	non-mucilage producing inner seed tissues	1172:1212	non-mucilage producing inner seed tissues	1172:1212	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	4	56	theme	substantial	885:895	arg1	differences					911:921	substantial interspecific differences	885:921	substantial interspecific differences in mucilage yield and macromolecular properties	885:969	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	4	56	theme	substantial	885:895	arg1	consequence					984:994	a consequence	982:994	a consequence of differences in heteroxylan and pectin composition	982:1047	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	4	57	from	differences	911:921	arg1	yield					935:939	mucilage yield	926:939	mucilage yield	926:939	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	4	57	from	differences	911:921	arg1	properties					960:969	macromolecular properties	945:969	macromolecular properties	945:969	We show that substantial interspecific differences in mucilage yield and macromolecular properties are mainly a consequence of differences in heteroxylan and pectin composition and probably represent wide differences in hydrocolloid functionality that can be exploited in industry.					
34135417	5	58	theme	producing	1185:1193	arg1	tissues					1206:1212	non-mucilage producing inner seed tissues	1172:1212	non-mucilage producing inner seed tissues	1172:1212	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	3	59	theme	crop	858:861	arg1	options					863:869	novel commercial crop options	841:869	novel commercial crop options	841:869	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	3	60	theme	nutritious	586:595	arg1	resource					597:604	a highly nutritious resource	577:604	a highly nutritious resource	577:604	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	3	61	theme	physicochemical	649:663	arg1	analyses					686:693	histological, physicochemical, and chromatographic analyses	635:693	histological, physicochemical, and chromatographic analyses	635:693	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	1	62	theme	Plantago	136:143	arg1	seeds					145:149	Plantago seeds	136:149	Plantago seeds	136:149	When wetted, Plantago seeds become covered with a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre.					
34135417	3	63	theme	harsh	794:798	arg1	conditions					811:820	harsh Australian conditions	794:820	harsh Australian conditions that may represent novel commercial crop options	794:869	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	5	64	from	sugars	1279:1284	arg1	high					1259:1262	high	1259:1262	high	1259:1262	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	1	65	contain	has	218:220	arg2	value					222:226	value	222:226	value	222:226	When wetted, Plantago seeds become covered with a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre.					
34135417	1	65	contain	has	218:220	arg1	gel					193:195	a polysaccharide-rich gel	171:195	a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre	171:271	When wetted, Plantago seeds become covered with a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre.					
34135417	1	65	contain	has	218:220	arg2	food					233:236	a food	231:236	a food additive and bulking dietary fibre	231:271	When wetted, Plantago seeds become covered with a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre.					
34135417	3	66	theme	novel	841:845	arg1	options					863:869	novel commercial crop options	841:869	novel commercial crop options	841:869	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	3	67	theme	Australian	800:809	arg1	conditions					811:820	harsh Australian conditions	794:820	harsh Australian conditions that may represent novel commercial crop options	794:869	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	5	68	theme	inner	1195:1199	arg1	tissues					1206:1212	non-mucilage producing inner seed tissues	1172:1212	non-mucilage producing inner seed tissues	1172:1212	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	3	69	theme	histological	635:646	arg1	analyses					686:693	histological, physicochemical, and chromatographic analyses	635:693	histological, physicochemical, and chromatographic analyses	635:693	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	6	70	theme	health	1443:1448	arg1	benefits					1450:1457	improved economic and health benefits	1421:1457	benefits	1450:1457	Whole seed Plantago flour, particularly from some species obtained from harsh Australian environments, may provide improved economic and health benefits compared to purified P. ovata psyllium husk, by retaining the functionality of the seed mucilage and providing additional essential nutrients.					
34135417	3	71	theme	commercial	847:856	arg1	options					863:869	novel commercial crop options	841:869	novel commercial crop options	841:869	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	1	72	theme	additive	238:245	arg1	value					222:226	value	222:226	value	222:226	When wetted, Plantago seeds become covered with a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre.					
34135417	1	72	theme	additive	238:245	arg1	food					233:236	a food	231:236	a food additive and bulking dietary fibre	231:271	When wetted, Plantago seeds become covered with a polysaccharide-rich gel called mucilage that has value as a food additive and bulking dietary fibre.					
34135417	5	73	theme	high	1259:1262	arg1	endosperm					1248:1256	a substantial mannan-rich endosperm	1222:1256	a substantial mannan-rich endosperm	1222:1256	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	3	74	theme	chromatographic	670:684	arg1	analyses					686:693	histological, physicochemical, and chromatographic analyses	635:693	histological, physicochemical, and chromatographic analyses	635:693	We suggest that this practice is potentially wasting a highly nutritious resource and here describe the use of histological, physicochemical, and chromatographic analyses to compare whole seed composition/characteristics of P. ovata with 11 relatives already adapted to harsh Australian conditions that may represent novel commercial crop options.					
34135417	2	75	dep	seeds	376:380	arg1	off					357:359	off	357:359	off	357:359	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
34135417	5	76	theme	fermentable	1267:1277	arg1	sugars					1279:1284	fermentable sugars	1267:1284	fermentable sugars	1267:1284	We also show that non-mucilage producing inner seed tissues contain a substantial mannan-rich endosperm, high in fermentable sugars, protein, and fats.					
34135417	2	77	theme	animal	498:503	arg1	feed					505:508	low value animal feed	488:508	low value animal feed	488:508	Industrially, the dry husk layer that becomes mucilage, called psyllium, is milled off Plantago ovata seeds, the only commercial-relevant Plantago species, while the residual inner seed tissues are either used for low value animal feed or discarded.					
35178379	2	0	theme	virus-cell	367:376	arg1	interaction					378:388	the virus-cell interaction	363:388	the virus-cell interaction	363:388	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	6	1	theme	Recombinant	967:977	arg1	RBD					979:981	Recombinant RBD	967:981	Recombinant RBD	967:981	Recombinant RBD was purified and the formation of complex N-glycans carrying blood group A antigens was shown by immunoblotting and MS analysis.					
35178379	8	2	theme	blood	1481:1485	arg1	structures					1503:1512	blood group A antigen structures	1481:1512	blood group A antigen structures	1481:1512	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	9	3	from	antigens	1625:1632	arg1	RBD					1648:1650	SARS-CoV-2 RBD	1637:1650	SARS-CoV-2 RBD	1637:1650	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	4	4	theme	recombinant	695:705	arg1	variants					707:714	recombinant variants	695:714	recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants	695:820	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	7	5	theme	conformation-dependent	1158:1179	arg1	antibody					1188:1195	the conformation-dependent CR3022 antibody	1154:1195	the conformation-dependent CR3022 antibody	1154:1195	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	9	6	attach	derived	1535:1541	arg2	IgGs					1530:1533	IgGs	1530:1533	IgGs derived from sera of non-infected RBD-negative blood group O individuals	1530:1606	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	9	6	attach	derived	1535:1541	arg1	sera					1548:1551	sera	1548:1551	sera of non-infected RBD-negative blood group O individuals	1548:1606	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	2	7	theme	specific	324:331	arg1	epitopes					345:352	specific immunogenic epitopes	324:352	specific immunogenic epitopes	324:352	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	1	8	theme	immune	186:191	arg1	evasion					193:199	immune evasion	186:199	immune evasion	186:199	Glycosylation of viral envelope proteins is important for infectivity and immune evasion.					
35178379	4	9	theme	glycoengineering	658:673	arg1	approach					675:682	an in vivo glycoengineering approach	647:682	an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants	647:820	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	7	10	theme	ACE2	1136:1139	arg1	receptor					1141:1148	the cellular ACE2 receptor	1123:1148	the cellular ACE2 receptor	1123:1148	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	5	11	theme	ABH	889:891	arg1	antigen					893:899	the blood group ABH antigen	873:899	the blood group ABH antigen formation	873:909	SARS-CoV-2 RBD and human glycosyltransferases for the blood group ABH antigen formation were transiently co-expressed in N. benthamiana leaves.					
35178379	8	12	theme	sera	1299:1302	arg1	Analysis					1287:1294	Analysis	1287:1294	Analysis of sera from RBD-positive and RBD-negative individuals	1287:1349	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	6	13	theme	complex	1017:1023	arg1	N-glycans					1025:1033	complex N-glycans	1017:1033	complex N-glycans carrying blood group A antigens	1017:1065	Recombinant RBD was purified and the formation of complex N-glycans carrying blood group A antigens was shown by immunoblotting and MS analysis.					
35178379	8	14	contain	have	1425:1428	arg2	antibodies					1430:1439	antibodies	1430:1439	antibodies that strongly bind to RBD modified with blood group A antigen structures	1430:1512	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	8	14	contain	have	1425:1428	arg1	individuals					1413:1423	non-infected RBD-negative blood group O individuals	1373:1423	non-infected RBD-negative blood group O individuals	1373:1423	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	2	15	theme	virus	400:404	arg1	transmission					406:417	virus transmission	400:417	virus transmission	400:417	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	8	16	theme	RBD-negative	1386:1397	arg1	individuals					1413:1423	non-infected RBD-negative blood group O individuals	1373:1423	non-infected RBD-negative blood group O individuals	1373:1423	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	9	17	theme	O	1594:1594	arg1	individuals					1596:1606	non-infected RBD-negative blood group O individuals	1556:1606	non-infected RBD-negative blood group O individuals	1556:1606	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	8	18	theme	RBD-positive	1309:1320	arg1	individuals					1339:1349	RBD-positive and RBD-negative individuals	1309:1349	RBD-positive and RBD-negative individuals	1309:1349	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	0	19	from	SARS-CoV-2	57:66	arg1	Production					10:19	Planta Production	3:19	Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures	3:109	In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.					
35178379	8	20	theme	group	1405:1409	arg1	individuals					1413:1423	non-infected RBD-negative blood group O individuals	1373:1423	non-infected RBD-negative blood group O individuals	1373:1423	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	4	21	dep	in	650:651	arg1	vivo					653:656	vivo	653:656	vivo	653:656	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	2	22	theme	spike	217:221	arg1	protein					223:229	The SARS-CoV-2 spike protein	202:229	The SARS-CoV-2 spike protein	202:229	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	2	22	theme	spike	217:221	arg1	glycosylated					242:253	glycosylated	242:253	glycosylated	242:253	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	7	23	theme	RBD	1213:1215	arg1	variants					1231:1238	the RBD glycosylation variants	1209:1238	the RBD glycosylation variants carrying blood group antigens	1209:1268	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	7	23	theme	RBD	1213:1215	arg1	functional					1275:1284	functional	1275:1284	functional	1275:1284	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	3	24	from	antibodies	602:611	arg1	sera					622:625	human sera	616:625	human sera	616:625	On recombinant viral antigens used as subunit vaccines or for serological assays, distinct glycan structures may enhance the immunogenicity and are recognized by naturally occurring antibodies in human sera.					
35178379	2	25	theme	host-derived	259:270	arg1	modifications					279:291	host-derived glycan modifications	259:291	host-derived glycan modifications	259:291	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	1	26	theme	viral	129:133	arg1	proteins					144:151	viral envelope proteins	129:151	viral envelope proteins	129:151	Glycosylation of viral envelope proteins is important for infectivity and immune evasion.					
35178379	9	27	theme	blood	1611:1615	arg1	antigens					1625:1632	blood group A antigens	1611:1632	blood group A antigens on SARS-CoV-2 RBD	1611:1650	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	4	28	theme	SARS-CoV-2	723:732	arg1	RBD					759:761	RBD	759:761	RBD	759:761	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	4	28	theme	SARS-CoV-2	723:732	arg1	domain					751:756	the SARS-CoV-2 receptor-binding domain	719:756	the SARS-CoV-2 receptor-binding domain (RBD)	719:762	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	5	29	theme	blood	877:881	arg1	antigen					893:899	the blood group ABH antigen	873:899	the blood group ABH antigen formation	873:909	SARS-CoV-2 RBD and human glycosyltransferases for the blood group ABH antigen formation were transiently co-expressed in N. benthamiana leaves.					
35178379	1	30	theme	proteins	144:151	arg1	Glycosylation					112:124	Glycosylation	112:124	Glycosylation of viral envelope proteins	112:151	Glycosylation of viral envelope proteins is important for infectivity and immune evasion.					
35178379	4	31	theme	domain	751:756	arg1	variants					707:714	recombinant variants	695:714	recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants	695:820	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	0	32	theme	Human	73:77	arg1	Structures					100:109	Human Blood Group A Glycan Structures	73:109	Human Blood Group A Glycan Structures	73:109	In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.					
35178379	9	33	theme	SARS-CoV-2	1637:1646	arg1	RBD					1648:1650	SARS-CoV-2 RBD	1637:1650	SARS-CoV-2 RBD	1637:1650	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	4	34	theme	benthamiana	803:813	arg1	plants					815:820	Nicotiana benthamiana plants	793:820	Nicotiana benthamiana plants	793:820	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	5	35	dep	benthamiana	947:957	arg1	leaves					959:964	leaves	959:964	leaves	959:964	SARS-CoV-2 RBD and human glycosyltransferases for the blood group ABH antigen formation were transiently co-expressed in N. benthamiana leaves.					
35178379	0	36	theme	Group	85:89	arg1	Structures					100:109	Human Blood Group A Glycan Structures	73:109	Human Blood Group A Glycan Structures	73:109	In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.					
35178379	6	37	theme	group	1050:1054	arg1	antigens					1058:1065	blood group A antigens	1044:1065	blood group A antigens	1044:1065	Recombinant RBD was purified and the formation of complex N-glycans carrying blood group A antigens was shown by immunoblotting and MS analysis.					
35178379	0	38	theme	Glycan	93:98	arg1	Structures					100:109	Human Blood Group A Glycan Structures	73:109	Human Blood Group A Glycan Structures	73:109	In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.					
35178379	7	39	theme	glycosylation	1217:1229	arg1	variants					1231:1238	the RBD glycosylation variants	1209:1238	the RBD glycosylation variants carrying blood group antigens	1209:1268	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	7	39	theme	glycosylation	1217:1229	arg1	functional					1275:1284	functional	1275:1284	functional	1275:1284	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	4	40	theme	group	775:779	arg1	antigens					781:788	blood group antigens	769:788	blood group antigens in Nicotiana benthamiana plants	769:820	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	8	41	theme	A	1493:1493	arg1	structures					1503:1512	blood group A antigen structures	1481:1512	blood group A antigen structures	1481:1512	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	7	42	theme	blood	1249:1253	arg1	antigens					1261:1268	blood group antigens	1249:1268	blood group antigens	1249:1268	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	9	43	from	degree	1702:1707	arg1	infection					1734:1742	virus infection	1728:1742	virus infection	1728:1742	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	1	44	theme	envelope	135:142	arg1	proteins					144:151	viral envelope proteins	129:151	viral envelope proteins	129:151	Glycosylation of viral envelope proteins is important for infectivity and immune evasion.					
35178379	4	45	with	variants	707:714	arg1	antigens					781:788	blood group antigens	769:788	blood group antigens in Nicotiana benthamiana plants	769:820	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	8	46	theme	RBD-negative	1326:1337	arg1	individuals					1339:1349	RBD-positive and RBD-negative individuals	1309:1349	RBD-positive and RBD-negative individuals	1309:1349	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	6	47	theme	MS	1099:1100	arg1	analysis					1102:1109	MS analysis	1099:1109	MS analysis	1099:1109	Recombinant RBD was purified and the formation of complex N-glycans carrying blood group A antigens was shown by immunoblotting and MS analysis.					
35178379	3	48	theme	recombinant	423:433	arg1	antigens					441:448	recombinant viral antigens	423:448	recombinant viral antigens used as subunit vaccines or for serological assays	423:499	On recombinant viral antigens used as subunit vaccines or for serological assays, distinct glycan structures may enhance the immunogenicity and are recognized by naturally occurring antibodies in human sera.					
35178379	6	49	contain	carrying	1035:1042	arg1	N-glycans					1025:1033	complex N-glycans	1017:1033	complex N-glycans carrying blood group A antigens	1017:1065	Recombinant RBD was purified and the formation of complex N-glycans carrying blood group A antigens was shown by immunoblotting and MS analysis.					
35178379	6	49	contain	carrying	1035:1042	arg2	antigens					1058:1065	blood group A antigens	1044:1065	blood group A antigens	1044:1065	Recombinant RBD was purified and the formation of complex N-glycans carrying blood group A antigens was shown by immunoblotting and MS analysis.					
35178379	5	50	theme	human	842:846	arg1	glycosyltransferases					848:867	human glycosyltransferases	842:867	human glycosyltransferases	842:867	SARS-CoV-2 RBD and human glycosyltransferases for the blood group ABH antigen formation were transiently co-expressed in N. benthamiana leaves.					
35178379	3	51	theme	distinct	502:509	arg1	structures					518:527	distinct glycan structures	502:527	distinct glycan structures	502:527	On recombinant viral antigens used as subunit vaccines or for serological assays, distinct glycan structures may enhance the immunogenicity and are recognized by naturally occurring antibodies in human sera.					
35178379	8	52	from	Analysis	1287:1294	arg1	individuals					1339:1349	RBD-positive and RBD-negative individuals	1309:1349	RBD-positive and RBD-negative individuals	1309:1349	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	7	53	theme	CR3022	1181:1186	arg1	antibody					1188:1195	the conformation-dependent CR3022 antibody	1154:1195	the conformation-dependent CR3022 antibody	1154:1195	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	9	54	theme	IgGs	1530:1533	arg1	binding					1519:1525	The binding	1515:1525	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD	1515:1650	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	0	55	with	SARS-CoV-2	57:66	arg1	Structures					100:109	Human Blood Group A Glycan Structures	73:109	Human Blood Group A Glycan Structures	73:109	In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.					
35178379	0	56	theme	Domain	45:50	arg1	Production					10:19	Planta Production	3:19	Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures	3:109	In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.					
35178379	2	57	dep	glycosylated	242:253	arg1	enhance					355:361	enhance	355:361	enhance the virus-cell interaction	355:388	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	2	57	dep	glycosylated	242:253	arg1	contribute					293:302	contribute	293:302	contribute to the formation of specific immunogenic epitopes	293:352	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	2	57	dep	glycosylated	242:253	arg1	affect					393:398	affect	393:398	affect virus transmission	393:417	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	7	58	contain	carrying	1240:1247	arg2	antigens					1261:1268	blood group antigens	1249:1268	blood group antigens	1249:1268	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	7	58	contain	carrying	1240:1247	arg1	variants					1231:1238	the RBD glycosylation variants	1209:1238	the RBD glycosylation variants carrying blood group antigens	1209:1268	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	7	58	contain	carrying	1240:1247	arg1	functional					1275:1284	functional	1275:1284	functional	1275:1284	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	2	59	theme	immunogenic	333:343	arg1	epitopes					345:352	specific immunogenic epitopes	324:352	specific immunogenic epitopes	324:352	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	6	60	theme	blood	1044:1048	arg1	antigens					1058:1065	blood group A antigens	1044:1065	blood group A antigens	1044:1065	Recombinant RBD was purified and the formation of complex N-glycans carrying blood group A antigens was shown by immunoblotting and MS analysis.					
35178379	2	61	gly	glycosylated	242:253	arg1	protein					223:229	The SARS-CoV-2 spike protein	202:229	The SARS-CoV-2 spike protein	202:229	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	2	61	gly	glycosylated	242:253	arg1	glycosylated					242:253	glycosylated	242:253	glycosylated	242:253	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	1	62	gly	Glycosylation	112:124	arg1	proteins					144:151	viral envelope proteins	129:151	viral envelope proteins	129:151	Glycosylation of viral envelope proteins is important for infectivity and immune evasion.					
35178379	3	63	theme	occurring	592:600	arg1	antibodies					602:611	naturally occurring antibodies	582:611	naturally occurring antibodies in human sera	582:625	On recombinant viral antigens used as subunit vaccines or for serological assays, distinct glycan structures may enhance the immunogenicity and are recognized by naturally occurring antibodies in human sera.					
35178379	5	64	theme	group	883:887	arg1	antigen					893:899	the blood group ABH antigen	873:899	the blood group ABH antigen formation	873:909	SARS-CoV-2 RBD and human glycosyltransferases for the blood group ABH antigen formation were transiently co-expressed in N. benthamiana leaves.					
35178379	3	65	theme	human	616:620	arg1	sera					622:625	human sera	616:625	human sera	616:625	On recombinant viral antigens used as subunit vaccines or for serological assays, distinct glycan structures may enhance the immunogenicity and are recognized by naturally occurring antibodies in human sera.					
35178379	6	66	theme	N-glycans	1025:1033	arg1	formation					1004:1012	the formation	1000:1012	the formation of complex N-glycans carrying blood group A antigens	1000:1065	Recombinant RBD was purified and the formation of complex N-glycans carrying blood group A antigens was shown by immunoblotting and MS analysis.					
35178379	9	67	theme	non-infected	1556:1567	arg1	individuals					1596:1606	non-infected RBD-negative blood group O individuals	1556:1606	non-infected RBD-negative blood group O individuals	1556:1606	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	5	68	theme	antigen	893:899	arg1	formation					901:909	the blood group ABH antigen formation	873:909	the blood group ABH antigen formation	873:909	SARS-CoV-2 RBD and human glycosyltransferases for the blood group ABH antigen formation were transiently co-expressed in N. benthamiana leaves.					
35178379	8	69	theme	non-infected	1373:1384	arg1	individuals					1413:1423	non-infected RBD-negative blood group O individuals	1373:1423	non-infected RBD-negative blood group O individuals	1373:1423	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	8	70	from	individuals	1339:1349	arg1	sera					1299:1302	sera	1299:1302	sera from RBD-positive and RBD-negative individuals	1299:1349	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	8	70	from	individuals	1339:1349	arg1	Analysis					1287:1294	Analysis	1287:1294	Analysis of sera from RBD-positive and RBD-negative individuals	1287:1349	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	9	71	theme	individuals	1596:1606	arg1	sera					1548:1551	sera	1548:1551	sera of non-infected RBD-negative blood group O individuals	1548:1606	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	7	72	theme	cellular	1127:1134	arg1	receptor					1141:1148	the cellular ACE2 receptor	1123:1148	the cellular ACE2 receptor	1123:1148	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
35178379	8	73	theme	blood	1399:1403	arg1	individuals					1413:1423	non-infected RBD-negative blood group O individuals	1373:1423	non-infected RBD-negative blood group O individuals	1373:1423	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	2	74	theme	glycan	272:277	arg1	modifications					279:291	host-derived glycan modifications	259:291	host-derived glycan modifications	259:291	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	0	75	theme	Receptor-Binding	28:43	arg1	Domain					45:50	the Receptor-Binding Domain	24:50	the Receptor-Binding Domain	24:50	In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.					
35178379	8	76	theme	O	1411:1411	arg1	individuals					1413:1423	non-infected RBD-negative blood group O individuals	1373:1423	non-infected RBD-negative blood group O individuals	1373:1423	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	9	77	theme	A	1623:1623	arg1	antigens					1625:1632	blood group A antigens	1611:1632	blood group A antigens on SARS-CoV-2 RBD	1611:1650	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	5	78	theme	N.	944:945	arg1	benthamiana					947:957	N. benthamiana	944:957	N. benthamiana leaves	944:964	SARS-CoV-2 RBD and human glycosyltransferases for the blood group ABH antigen formation were transiently co-expressed in N. benthamiana leaves.					
35178379	0	79	theme	Blood	79:83	arg1	Structures					100:109	Human Blood Group A Glycan Structures	73:109	Human Blood Group A Glycan Structures	73:109	In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.					
35178379	4	80	theme	receptor-binding	734:749	arg1	RBD					759:761	RBD	759:761	RBD	759:761	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	4	80	theme	receptor-binding	734:749	arg1	domain					751:756	the SARS-CoV-2 receptor-binding domain	719:756	the SARS-CoV-2 receptor-binding domain (RBD)	719:762	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	0	81	theme	A	91:91	arg1	Structures					100:109	Human Blood Group A Glycan Structures	73:109	Human Blood Group A Glycan Structures	73:109	In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.					
35178379	6	82	theme	A	1056:1056	arg1	antigens					1058:1065	blood group A antigens	1044:1065	blood group A antigens	1044:1065	Recombinant RBD was purified and the formation of complex N-glycans carrying blood group A antigens was shown by immunoblotting and MS analysis.					
35178379	9	83	theme	group	1617:1621	arg1	antigens					1625:1632	blood group A antigens	1611:1632	blood group A antigens on SARS-CoV-2 RBD	1611:1650	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	4	84	theme	Nicotiana	793:801	arg1	plants					815:820	Nicotiana benthamiana plants	793:820	Nicotiana benthamiana plants	793:820	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	9	85	theme	RBD-negative	1569:1580	arg1	individuals					1596:1606	non-infected RBD-negative blood group O individuals	1556:1606	non-infected RBD-negative blood group O individuals	1556:1606	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	3	86	theme	glycan	511:516	arg1	structures					518:527	distinct glycan structures	502:527	distinct glycan structures	502:527	On recombinant viral antigens used as subunit vaccines or for serological assays, distinct glycan structures may enhance the immunogenicity and are recognized by naturally occurring antibodies in human sera.					
35178379	9	87	theme	blood	1582:1586	arg1	individuals					1596:1606	non-infected RBD-negative blood group O individuals	1556:1606	non-infected RBD-negative blood group O individuals	1556:1606	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	2	88	link	host-derived	259:270	arg1	modifications					279:291	host-derived glycan modifications	259:291	host-derived glycan modifications	259:291	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	0	89	theme	Planta	3:8	arg1	Production					10:19	Planta Production	3:19	Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures	3:109	In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures.					
35178379	4	90	from	antigens	781:788	arg1	plants					815:820	Nicotiana benthamiana plants	793:820	Nicotiana benthamiana plants	793:820	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	9	91	from	infection	1734:1742	arg1	protection					1712:1721	protection	1712:1721	protection from virus infection	1712:1742	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	9	91	from	infection	1734:1742	arg1	degree					1702:1707	some degree	1697:1707	some degree of protection from virus infection	1697:1742	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	4	92	theme	blood	769:773	arg1	antigens					781:788	blood group antigens	769:788	blood group antigens in Nicotiana benthamiana plants	769:820	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	3	93	theme	subunit	458:464	arg1	vaccines					466:473	subunit vaccines	458:473	subunit vaccines	458:473	On recombinant viral antigens used as subunit vaccines or for serological assays, distinct glycan structures may enhance the immunogenicity and are recognized by naturally occurring antibodies in human sera.					
35178379	8	94	theme	group	1487:1491	arg1	structures					1503:1512	blood group A antigen structures	1481:1512	blood group A antigen structures	1481:1512	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	5	95	theme	SARS-CoV-2	823:832	arg1	RBD					834:836	SARS-CoV-2 RBD	823:836	SARS-CoV-2 RBD	823:836	SARS-CoV-2 RBD and human glycosyltransferases for the blood group ABH antigen formation were transiently co-expressed in N. benthamiana leaves.					
35178379	9	96	theme	group	1588:1592	arg1	individuals					1596:1606	non-infected RBD-negative blood group O individuals	1556:1606	non-infected RBD-negative blood group O individuals	1556:1606	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	8	97	theme	antigen	1495:1501	arg1	structures					1503:1512	blood group A antigen structures	1481:1512	blood group A antigen structures	1481:1512	Analysis of sera from RBD-positive and RBD-negative individuals revealed further that non-infected RBD-negative blood group O individuals have antibodies that strongly bind to RBD modified with blood group A antigen structures.					
35178379	3	98	theme	serological	482:492	arg1	assays					494:499	serological assays	482:499	serological assays	482:499	On recombinant viral antigens used as subunit vaccines or for serological assays, distinct glycan structures may enhance the immunogenicity and are recognized by naturally occurring antibodies in human sera.					
35178379	9	99	theme	protection	1712:1721	arg1	degree					1702:1707	some degree	1697:1707	some degree of protection from virus infection	1697:1742	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	3	100	theme	viral	435:439	arg1	antigens					441:448	recombinant viral antigens	423:448	recombinant viral antigens used as subunit vaccines or for serological assays	423:499	On recombinant viral antigens used as subunit vaccines or for serological assays, distinct glycan structures may enhance the immunogenicity and are recognized by naturally occurring antibodies in human sera.					
35178379	2	101	theme	SARS-CoV-2	206:215	arg1	protein					223:229	The SARS-CoV-2 spike protein	202:229	The SARS-CoV-2 spike protein	202:229	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	2	101	theme	SARS-CoV-2	206:215	arg1	glycosylated					242:253	glycosylated	242:253	glycosylated	242:253	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	9	102	theme	virus	1728:1732	arg1	infection					1734:1742	virus infection	1728:1742	virus infection	1728:1742	The binding of IgGs derived from sera of non-infected RBD-negative blood group O individuals to blood group A antigens on SARS-CoV-2 RBD suggests that these antibodies could provide some degree of protection from virus infection.					
35178379	2	103	theme	epitopes	345:352	arg1	formation					311:319	the formation	307:319	the formation of specific immunogenic epitopes	307:352	The SARS-CoV-2 spike protein is heavily glycosylated and host-derived glycan modifications contribute to the formation of specific immunogenic epitopes, enhance the virus-cell interaction or affect virus transmission.					
35178379	4	104	theme	in	650:651	arg1	approach					675:682	an in vivo glycoengineering approach	647:682	an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants	647:820	Here, we performed an in vivo glycoengineering approach to produce recombinant variants of the SARS-CoV-2 receptor-binding domain (RBD) with blood group antigens in Nicotiana benthamiana plants.					
35178379	7	105	theme	group	1255:1259	arg1	antigens					1261:1268	blood group antigens	1249:1268	blood group antigens	1249:1268	Binding to the cellular ACE2 receptor and the conformation-dependent CR3022 antibody showed that the RBD glycosylation variants carrying blood group antigens were functional.					
32089269	8	0	theme	93.1	1203:1206	arg1	viability					1190:1198	cell viability	1185:1198	cell viability of 93.1	1185:1206	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	10	1	with	contact	1491:1497	arg1	particles					1537:1545	exposed chitosan particles	1520:1545	exposed chitosan particles on the surface	1520:1560	No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect.					
32089269	10	2	theme	chitosan	1528:1535	arg1	particles					1537:1545	exposed chitosan particles	1520:1545	exposed chitosan particles on the surface	1520:1560	No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect.					
32089269	1	3	contain	has	182:184	arg2	potential					190:198	the potential to slow the development of carious lesions	186:241	the potential to slow the development of carious lesions	186:241	The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions.					
32089269	1	3	contain	has	182:184	arg1	incorporation					111:123	The incorporation	107:123	The incorporation of antimicrobial agents in restorative dental composites	107:180	The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions.					
32089269	9	4	with	composites	1335:1344	arg1	biofilm					1374:1380	approximately 20% less biofilm	1351:1380	approximately 20% less biofilm (p < 0.001) relative to the control	1351:1416	The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control.					
32089269	9	4	with	composites	1335:1344	arg1	<					1385:1385	p < 0.001	1383:1391	p < 0.001	1383:1391	The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control.					
32089269	2	5	theme	study	284:288	arg1	objectives					258:267	The objectives	254:267	The objectives of the present study	254:288	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	12	6	from	addition	1942:1949	arg1	composites					2009:2018	the restorative composites	1993:2018	the restorative composites	1993:2018	SIGNIFICANCE It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.					
32089269	8	7	theme	micronucleus	1242:1253	arg1	%					1240:1240	3.0 ± 0.8%	1231:1240	3.0 ± 0.8% micronucleus per group (p = 0.1078)	1231:1276	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	8	8	theme	=	1218:1218	arg1	p					1216:1216	p = 0.328	1216:1224	p = 0.328	1216:1224	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	8	8	theme	=	1218:1218	arg1	%					1213:1213	8.0%	1210:1213	8.0% (p = 0.328)	1210:1225	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	2	9	theme	experimental	306:317	arg1	resins					329:334	experimental composite resins	306:334	experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles	306:425	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	5	10	theme	aging	844:848	arg1	days					836:839	1 and 90 days	827:839	1 and 90 days of aging	827:848	The degree of conversion and mechanical properties were measured after 1 and 90 days of aging in water after photoactivation.					
32089269	10	11	theme	antimicrobial	1595:1607	arg1	effect					1609:1614	the observed antimicrobial effect	1582:1614	the observed antimicrobial effect	1582:1614	No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect.					
32089269	4	12	theme	wt	690:691	arg1	particles					694:702	0, 0.5, or 1.0 wt% particles	675:702	0, 0.5, or 1.0 wt% particles	675:702	Experimental composites were formulated by adding 0, 0.5, or 1.0 wt% particles into a resin matrix along with 60 wt% barium glass.					
32089269	7	13	theme	crystal	1057:1063	arg1	assay					1080:1084	crystal violet biofilm assay	1057:1084	crystal violet biofilm assay	1057:1084	The antimicrobial activity against Streptococcus mutans was assessed by crystal violet biofilm assay.					
32089269	1	14	theme	carious	227:233	arg1	lesions					235:241	carious lesions	227:241	carious lesions	227:241	The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions.					
32089269	6	15	theme	conditioned	965:975	arg1	medium					977:982	conditioned medium	965:982	conditioned medium	965:982	Cytotoxicity and genotoxicity were evaluated using fibroblasts from dental pulp in conditioned medium.					
32089269	11	16	from	aging	1884:1888	arg1	water					1893:1897	water	1893:1897	water	1893:1897	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	4	17	theme	wt	738:739	arg1	glass					749:753	60 wt% barium glass	735:753	60 wt% barium glass	735:753	Experimental composites were formulated by adding 0, 0.5, or 1.0 wt% particles into a resin matrix along with 60 wt% barium glass.					
32089269	1	18	theme	antimicrobial	128:140	arg1	agents					142:147	antimicrobial agents	128:147	antimicrobial agents	128:147	The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions.					
32089269	4	19	theme	barium	742:747	arg1	glass					749:753	60 wt% barium glass	735:753	60 wt% barium glass	735:753	Experimental composites were formulated by adding 0, 0.5, or 1.0 wt% particles into a resin matrix along with 60 wt% barium glass.					
32089269	5	20	theme	conversion	770:779	arg1	degree					760:765	The degree	756:765	The degree of conversion and mechanical properties	756:805	The degree of conversion and mechanical properties were measured after 1 and 90 days of aging in water after photoactivation.					
32089269	1	21	theme	dental	164:169	arg1	composites					171:180	restorative dental composites	152:180	restorative dental composites	152:180	The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions.					
32089269	2	22	theme	biocompatibility	517:532	arg1	loss					484:487	loss	484:487	loss of mechanical properties or biocompatibility	484:532	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	12	23	theme	antimicrobial	2028:2040	arg1	activity					2042:2049	antimicrobial activity	2028:2049	antimicrobial activity	2028:2049	SIGNIFICANCE It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.					
32089269	5	24	theme	mechanical	785:794	arg1	properties					796:805	mechanical properties	785:805	mechanical properties	785:805	The degree of conversion and mechanical properties were measured after 1 and 90 days of aging in water after photoactivation.					
32089269	7	25	theme	antimicrobial	989:1001	arg1	activity					1003:1010	The antimicrobial activity	985:1010	The antimicrobial activity against Streptococcus mutans	985:1039	The antimicrobial activity against Streptococcus mutans was assessed by crystal violet biofilm assay.					
32089269	9	26	dep	showed	1319:1324	arg1	composites					1335:1344	all composites	1331:1344	all composites with approximately 20% less biofilm (p < 0.001) relative to the control	1331:1416	The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control.					
32089269	12	27	dep	SIGNIFICANCE	1900:1911	arg1	It					1913:1914	It	1913:1914	It	1913:1914	SIGNIFICANCE It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.					
32089269	2	28	with	resins	329:334	arg1	chitosan					353:360	chitosan	353:360	chitosan	353:360	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	2	28	with	resins	329:334	arg1	chitosan					341:348	chitosan	341:348	chitosan	341:348	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	0	29	theme	composites	40:49	arg1	Development					0:10	Development	0:10	Development of novel dental restorative composites with dibasic calcium phosphate	0:80	Development of novel dental restorative composites with dibasic calcium phosphate loaded chitosan fillers.					
32089269	3	30	dep	METHODS	535:541	arg1	Chitosan					543:550	Chitosan	543:550	Chitosan	543:550	METHODS Chitosan and chitosan/DCPA particles were synthetized by the electrospray method.					
32089269	3	30	dep	METHODS	535:541	arg1	particles					570:578	chitosan/DCPA particles	556:578	chitosan/DCPA particles	556:578	METHODS Chitosan and chitosan/DCPA particles were synthetized by the electrospray method.					
32089269	11	31	theme	control	1856:1862	arg1	group					1864:1868	the control group	1852:1868	the control group	1852:1868	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	8	32	theme	restorative	1112:1122	arg1	cytotoxic					1156:1164	cytotoxic	1156:1164	cytotoxic	1156:1164	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	8	32	theme	restorative	1112:1122	arg1	composites					1124:1133	The experimental restorative composites	1095:1133	The experimental restorative composites	1095:1133	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	2	33	theme	antimicrobial	452:464	arg1	potential					466:474	their antimicrobial potential	446:474	their antimicrobial potential	446:474	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	0	34	theme	novel	15:19	arg1	composites					40:49	novel dental restorative composites	15:49	novel dental restorative composites	15:49	Development of novel dental restorative composites with dibasic calcium phosphate loaded chitosan fillers.					
32089269	2	35	theme	properties	503:512	arg1	loss					484:487	loss	484:487	loss of mechanical properties or biocompatibility	484:532	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	0	36	theme	calcium	64:70	arg1	phosphate					72:80	dibasic calcium phosphate	56:80	dibasic calcium phosphate	56:80	Development of novel dental restorative composites with dibasic calcium phosphate loaded chitosan fillers.					
32089269	0	37	theme	restorative	28:38	arg1	composites					40:49	novel dental restorative composites	15:49	novel dental restorative composites	15:49	Development of novel dental restorative composites with dibasic calcium phosphate loaded chitosan fillers.					
32089269	10	38	theme	direct	1484:1489	arg1	contact					1491:1497	direct contact	1484:1497	direct contact of the bacteria with exposed chitosan particles on the surface	1484:1560	No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect.					
32089269	11	39	theme	chitosan/DCPA	1650:1662	arg1	submicrometer					1664:1676	chitosan/DCPA submicrometer	1650:1676	chitosan/DCPA submicrometer (<250 nm average diameter)	1650:1703	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	11	39	theme	chitosan/DCPA	1650:1662	arg1	diameter					1695:1702	<250 nm average diameter	1679:1702	<250 nm average diameter	1679:1702	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	0	40	with	Development	0:10	arg1	phosphate					72:80	dibasic calcium phosphate	56:80	dibasic calcium phosphate	56:80	Development of novel dental restorative composites with dibasic calcium phosphate loaded chitosan fillers.					
32089269	11	41	theme	elastic	1801:1807	arg1	conversion					1770:1779	conversion	1770:1779	conversion	1770:1779	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	11	41	theme	elastic	1801:1807	arg1	modulus					1809:1815	elastic modulus	1801:1815	elastic modulus	1801:1815	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	1	42	from	incorporation	111:123	arg1	composites					171:180	restorative dental composites	152:180	restorative dental composites	152:180	The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions.					
32089269	1	43	theme	lesions	235:241	arg1	development					212:222	the development	208:222	the development of carious lesions	208:241	The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions.					
32089269	10	44	from	particles	1537:1545	arg1	surface					1554:1560	the surface	1550:1560	the surface	1550:1560	No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect.					
32089269	12	45	theme	restorative	1997:2007	arg1	composites					2009:2018	the restorative composites	1993:2018	the restorative composites	1993:2018	SIGNIFICANCE It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.					
32089269	10	46	theme	bacteria	1506:1513	arg1	contact					1491:1497	direct contact	1484:1497	direct contact of the bacteria with exposed chitosan particles on the surface	1484:1560	No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect.					
32089269	11	47	theme	nm	1684:1685	arg1	submicrometer					1664:1676	chitosan/DCPA submicrometer	1650:1676	chitosan/DCPA submicrometer (<250 nm average diameter)	1650:1703	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	11	47	theme	nm	1684:1685	arg1	diameter					1695:1702	<250 nm average diameter	1679:1702	<250 nm average diameter	1679:1702	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	3	48	theme	chitosan/DCPA	556:568	arg1	particles					570:578	chitosan/DCPA particles	556:578	chitosan/DCPA particles	556:578	METHODS Chitosan and chitosan/DCPA particles were synthetized by the electrospray method.					
32089269	8	49	theme	±	1235:1235	arg1	%					1240:1240	3.0 ± 0.8%	1231:1240	3.0 ± 0.8% micronucleus per group (p = 0.1078)	1231:1276	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	12	50	theme	chitosan/DCPA	1966:1978	arg1	particles					1980:1988	chitosan/DCPA particles	1966:1988	chitosan/DCPA particles	1966:1988	SIGNIFICANCE It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.					
32089269	6	51	theme	dental	950:955	arg1	pulp					957:960	dental pulp	950:960	dental pulp in conditioned medium	950:982	Cytotoxicity and genotoxicity were evaluated using fibroblasts from dental pulp in conditioned medium.					
32089269	2	52	theme	dibasic	374:380	arg1	DCPA					411:414	DCPA	411:414	DCPA	411:414	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	2	52	theme	dibasic	374:380	arg1	anhydrous					400:408	dibasic calcium phosphate anhydrous	374:408	dibasic calcium phosphate anhydrous (DCPA) particles	374:425	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	9	53	theme	p	1383:1383	arg1	biofilm					1374:1380	approximately 20% less biofilm	1351:1380	approximately 20% less biofilm (p < 0.001) relative to the control	1351:1416	The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control.					
32089269	9	53	theme	p	1383:1383	arg1	<					1385:1385	p < 0.001	1383:1391	p < 0.001	1383:1391	The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control.					
32089269	8	54	theme	cell	1185:1188	arg1	viability					1190:1198	cell viability	1185:1198	cell viability of 93.1	1185:1206	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	8	55	dep	RESULTS	1087:1093	arg1	found					1144:1148	found	1144:1148	found to be cytotoxic or genotoxic	1144:1177	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	10	56	theme	exposed	1520:1526	arg1	particles					1537:1545	exposed chitosan particles	1520:1545	exposed chitosan particles on the surface	1520:1560	No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect.					
32089269	11	57	theme	chitosan	1637:1644	arg1	particles					1705:1713	the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles	1633:1713	the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles	1633:1713	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	9	58	theme	relative	1394:1401	arg1	biofilm					1374:1380	approximately 20% less biofilm	1351:1380	approximately 20% less biofilm (p < 0.001) relative to the control	1351:1416	The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control.					
32089269	9	58	theme	relative	1394:1401	arg1	<					1385:1385	p < 0.001	1383:1391	p < 0.001	1383:1391	The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control.					
32089269	12	59	theme	chitosan	1954:1961	arg1	addition					1942:1949	the addition	1938:1949	the addition of chitosan or chitosan/DCPA particles in the restorative composites	1938:2018	SIGNIFICANCE It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.					
32089269	4	60	theme	%	692:692	arg1	particles					694:702	0, 0.5, or 1.0 wt% particles	675:702	0, 0.5, or 1.0 wt% particles	675:702	Experimental composites were formulated by adding 0, 0.5, or 1.0 wt% particles into a resin matrix along with 60 wt% barium glass.					
32089269	12	61	theme	composites	2125:2134	arg1	biocompatibility					2101:2116	biocompatibility	2101:2116	biocompatibility of the composites	2101:2134	SIGNIFICANCE It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.					
32089269	12	61	theme	composites	2125:2134	arg1	properties					2087:2096	the mechanical properties	2072:2096	the mechanical properties	2072:2096	SIGNIFICANCE It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.					
32089269	11	62	theme	conversion	1770:1779	arg1	degree					1760:1765	the degree	1756:1765	the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group	1756:1868	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	3	63	theme	electrospray	604:615	arg1	method					617:622	the electrospray method	600:622	the electrospray method	600:622	METHODS Chitosan and chitosan/DCPA particles were synthetized by the electrospray method.					
32089269	12	64	theme	mechanical	2076:2085	arg1	properties					2087:2096	the mechanical properties	2072:2096	the mechanical properties	2072:2096	SIGNIFICANCE It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.					
32089269	10	65	theme	observed	1586:1593	arg1	effect					1609:1614	the observed antimicrobial effect	1582:1614	the observed antimicrobial effect	1582:1614	No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect.					
32089269	2	66	dep	OBJECTIVE	244:252	arg1	were					290:293	were	290:293	were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility	290:532	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	7	67	theme	violet	1065:1070	arg1	assay					1080:1084	crystal violet biofilm assay	1057:1084	crystal violet biofilm assay	1057:1084	The antimicrobial activity against Streptococcus mutans was assessed by crystal violet biofilm assay.					
32089269	8	68	theme	=	1268:1268	arg1	group					1259:1263	group	1259:1263	group (p = 0.1078)	1259:1276	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	8	68	theme	=	1268:1268	arg1	p					1266:1266	p = 0.1078	1266:1275	p = 0.1078	1266:1275	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	11	69	theme	flexural	1782:1789	arg1	conversion					1770:1779	conversion	1770:1779	conversion	1770:1779	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	11	69	theme	flexural	1782:1789	arg1	strength					1791:1798	flexural strength	1782:1798	flexural strength	1782:1798	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	7	70	theme	biofilm	1072:1078	arg1	assay					1080:1084	crystal violet biofilm assay	1057:1084	crystal violet biofilm assay	1057:1084	The antimicrobial activity against Streptococcus mutans was assessed by crystal violet biofilm assay.					
32089269	2	71	theme	present	276:282	arg1	study					284:288	the present study	272:288	the present study	272:288	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	1	72	theme	agents	142:147	arg1	incorporation					111:123	The incorporation	107:123	The incorporation of antimicrobial agents in restorative dental composites	107:180	The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions.					
32089269	2	73	theme	composite	319:327	arg1	resins					329:334	experimental composite resins	306:334	experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles	306:425	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	4	74	theme	%	740:740	arg1	glass					749:753	60 wt% barium glass	735:753	60 wt% barium glass	735:753	Experimental composites were formulated by adding 0, 0.5, or 1.0 wt% particles into a resin matrix along with 60 wt% barium glass.					
32089269	11	75	theme	restorative	1718:1728	arg1	composites					1730:1739	restorative composites	1718:1739	restorative composites	1718:1739	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	1	76	theme	restorative	152:162	arg1	composites					171:180	restorative dental composites	152:180	restorative dental composites	152:180	The incorporation of antimicrobial agents in restorative dental composites has the potential to slow the development of carious lesions.					
32089269	4	77	theme	resin	711:715	arg1	matrix					717:722	a resin matrix	709:722	a resin matrix along with 60 wt% barium glass	709:753	Experimental composites were formulated by adding 0, 0.5, or 1.0 wt% particles into a resin matrix along with 60 wt% barium glass.					
32089269	11	78	theme	particles	1705:1713	arg1	addition					1621:1628	The addition	1617:1628	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites	1617:1739	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	0	79	theme	chitosan	89:96	arg1	fillers					98:104	chitosan fillers	89:104	chitosan fillers	89:104	Development of novel dental restorative composites with dibasic calcium phosphate loaded chitosan fillers.					
32089269	7	80	theme	Streptococcus	1020:1032	arg1	mutans					1034:1039	Streptococcus mutans	1020:1039	Streptococcus mutans	1020:1039	The antimicrobial activity against Streptococcus mutans was assessed by crystal violet biofilm assay.					
32089269	8	81	theme	experimental	1099:1110	arg1	cytotoxic					1156:1164	cytotoxic	1156:1164	cytotoxic	1156:1164	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	8	81	theme	experimental	1099:1110	arg1	composites					1124:1133	The experimental restorative composites	1095:1133	The experimental restorative composites	1095:1133	RESULTS The experimental restorative composites were not found to be cytotoxic or genotoxic, with cell viability of 93.1 ± 8.0% (p = 0.328) and 3.0 ± 0.8% micronucleus per group (p = 0.1078), respectively.					
32089269	10	82	theme	chitosan	1422:1429	arg1	release					1431:1437	No chitosan release	1419:1437	No chitosan release	1419:1437	No chitosan release was detected from the composites, suggesting direct contact of the bacteria with exposed chitosan particles on the surface was responsible for the observed antimicrobial effect.					
32089269	9	83	theme	antimicrobial	1297:1309	arg1	results					1311:1317	The antimicrobial results	1293:1317	The antimicrobial results	1293:1317	The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control.					
32089269	2	84	theme	anhydrous	400:408	arg1	particles					417:425	dibasic calcium phosphate anhydrous (DCPA) particles	374:425	dibasic calcium phosphate anhydrous (DCPA) particles	374:425	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	0	85	theme	dental	21:26	arg1	composites					40:49	novel dental restorative composites	15:49	novel dental restorative composites	15:49	Development of novel dental restorative composites with dibasic calcium phosphate loaded chitosan fillers.					
32089269	4	86	theme	Experimental	625:636	arg1	composites					638:647	Experimental composites	625:647	Experimental composites	625:647	Experimental composites were formulated by adding 0, 0.5, or 1.0 wt% particles into a resin matrix along with 60 wt% barium glass.					
32089269	11	87	theme	submicrometer	1664:1676	arg1	particles					1705:1713	the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles	1633:1713	the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles	1633:1713	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	2	88	theme	phosphate	390:398	arg1	DCPA					411:414	DCPA	411:414	DCPA	411:414	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	2	88	theme	phosphate	390:398	arg1	anhydrous					400:408	dibasic calcium phosphate anhydrous	374:408	dibasic calcium phosphate anhydrous (DCPA) particles	374:425	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	5	89	theme	properties	796:805	arg1	degree					760:765	The degree	756:765	The degree of conversion and mechanical properties	756:805	The degree of conversion and mechanical properties were measured after 1 and 90 days of aging in water after photoactivation.					
32089269	2	90	theme	mechanical	492:501	arg1	properties					503:512	mechanical properties	492:512	mechanical properties	492:512	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	0	91	theme	dibasic	56:62	arg1	phosphate					72:80	dibasic calcium phosphate	56:80	dibasic calcium phosphate	56:80	Development of novel dental restorative composites with dibasic calcium phosphate loaded chitosan fillers.					
32089269	6	92	from	pulp	957:960	arg1	fibroblasts					933:943	fibroblasts	933:943	fibroblasts from dental pulp in conditioned medium	933:982	Cytotoxicity and genotoxicity were evaluated using fibroblasts from dental pulp in conditioned medium.					
32089269	6	92	from	pulp	957:960	arg1	medium					977:982	conditioned medium	965:982	conditioned medium	965:982	Cytotoxicity and genotoxicity were evaluated using fibroblasts from dental pulp in conditioned medium.					
32089269	11	93	theme	average	1687:1693	arg1	submicrometer					1664:1676	chitosan/DCPA submicrometer	1650:1676	chitosan/DCPA submicrometer (<250 nm average diameter)	1650:1703	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	11	93	theme	average	1687:1693	arg1	diameter					1695:1702	<250 nm average diameter	1679:1702	<250 nm average diameter	1679:1702	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	11	94	theme	fracture	1821:1828	arg1	conversion					1770:1779	conversion	1770:1779	conversion	1770:1779	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	11	94	theme	fracture	1821:1828	arg1	toughness					1830:1838	fracture toughness	1821:1838	fracture toughness	1821:1838	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	11	95	dep	days	1879:1882	arg1	aging					1884:1888	aging	1884:1888	90 days aging in water	1876:1897	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	11	96	theme	<250	1679:1682	arg1	nm					1684:1685	nm	1684:1685	nm	1684:1685	The addition of the chitosan and chitosan/DCPA submicrometer (<250 nm average diameter) particles to restorative composites did not change the degree of conversion, flexural strength, elastic modulus and fracture toughness compared to the control group after 90 days aging in water.					
32089269	9	97	theme	less	1369:1372	arg1	biofilm					1374:1380	approximately 20% less biofilm	1351:1380	approximately 20% less biofilm (p < 0.001) relative to the control	1351:1416	The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control.					
32089269	9	97	theme	less	1369:1372	arg1	<					1385:1385	p < 0.001	1383:1391	p < 0.001	1383:1391	The antimicrobial results showed that all composites with approximately 20% less biofilm (p < 0.001) relative to the control.					
32089269	12	98	theme	particles	1980:1988	arg1	addition					1942:1949	the addition	1938:1949	the addition of chitosan or chitosan/DCPA particles in the restorative composites	1938:2018	SIGNIFICANCE It can be concluded that the addition of chitosan or chitosan/DCPA particles in the restorative composites induced antimicrobial activity without compromising the mechanical properties or biocompatibility of the composites.					
32089269	2	99	theme	calcium	382:388	arg1	DCPA					411:414	DCPA	411:414	DCPA	411:414	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
32089269	2	99	theme	calcium	382:388	arg1	anhydrous					400:408	dibasic calcium phosphate anhydrous	374:408	dibasic calcium phosphate anhydrous (DCPA) particles	374:425	OBJECTIVE The objectives of the present study were to develop experimental composite resins with chitosan or chitosan loaded with dibasic calcium phosphate anhydrous (DCPA) particles and to demonstrate their antimicrobial potential without loss of mechanical properties or biocompatibility.					
34911759	10	0	from	chiralities	1713:1723	arg1	wall					1750:1753	the cell wall	1741:1753	the cell wall	1741:1753	Our findings, combined with mechanical simulation, suggest that the different chiralities of helicoids in the cell wall may result from different chemical composition, which strengthens previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils.					
34911759	6	1	theme	Pollia	843:848	arg1	fruit					861:865	the Pollia condensata fruit	839:865	the Pollia condensata fruit	839:865	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	10	2	theme	different	1771:1779	arg1	composition					1790:1800	different chemical composition	1771:1800	different chemical composition	1771:1800	Our findings, combined with mechanical simulation, suggest that the different chiralities of helicoids in the cell wall may result from different chemical composition, which strengthens previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils.					
34911759	7	3	theme	wall	1143:1146	arg1	composition					1148:1158	cell wall composition	1138:1158	cell wall composition	1138:1158	By simultaneously studying optical and mechanical responses of cells with different handednesses, we propose that the chirality of helicoids results from differences in cell wall composition.					
34911759	8	4	theme	observations	1233:1244	arg1	substantiation					1199:1212	statistical substantiation	1187:1212	statistical substantiation of three different observations	1187:1244	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	4	5	theme	plant	592:596	arg1	walls					603:607	plant cell walls	592:607	plant cell walls	592:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	2	6	theme	hierarchical	318:329	arg1	structures					331:340	complex hierarchical structures	310:340	complex hierarchical structures	310:340	While several natural chiral biomolecules exist only with single handedness, they can produce complex hierarchical structures with opposite chiralities.					
34911759	10	7	theme	previous	1821:1828	arg1	hypotheses					1830:1839	previous hypotheses	1821:1839	previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils	1821:1912	Our findings, combined with mechanical simulation, suggest that the different chiralities of helicoids in the cell wall may result from different chemical composition, which strengthens previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils.					
34911759	7	8	theme	helicoids	1100:1108	arg1	chirality					1087:1095	the chirality	1083:1095	the chirality of helicoids	1083:1108	By simultaneously studying optical and mechanical responses of cells with different handednesses, we propose that the chirality of helicoids results from differences in cell wall composition.					
34911759	2	9	theme	chiral	238:243	arg1	biomolecules					245:256	several natural chiral biomolecules	222:256	several natural chiral biomolecules	222:256	While several natural chiral biomolecules exist only with single handedness, they can produce complex hierarchical structures with opposite chiralities.					
34911759	4	10	theme	intriguing	482:491	arg1	transfer					508:515	the transfer	504:515	the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls	504:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	4	10	theme	intriguing	482:491	arg1	example					493:499	An intriguing example	479:499	An intriguing example	479:499	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	5	11	theme	light	724:728	arg1	wavelength					702:711	the wavelength	698:711	the wavelength of visible light	698:728	These cellulose helicoids produce structural colors if their dimension is comparable to the wavelength of visible light.					
34911759	2	12	theme	several	222:228	arg1	biomolecules					245:256	several natural chiral biomolecules	222:256	several natural chiral biomolecules	222:256	While several natural chiral biomolecules exist only with single handedness, they can produce complex hierarchical structures with opposite chiralities.					
34911759	7	13	theme	different	1043:1051	arg1	handednesses					1053:1064	different handednesses	1043:1064	different handednesses	1043:1064	By simultaneously studying optical and mechanical responses of cells with different handednesses, we propose that the chirality of helicoids results from differences in cell wall composition.					
34911759	8	14	dep	located	1450:1456	arg1	3					1424:1424	3	1424:1424	3	1424:1424	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	6	15	theme	tissue	961:966	arg1	cells					943:947	neighboring cells	931:947	neighboring cells of the same tissue	931:966	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	8	16	theme	interlocular	1493:1504	arg1	divisions					1506:1514	interlocular divisions	1493:1514	interlocular divisions	1493:1514	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	7	17	theme	cells	1032:1036	arg1	responses					1019:1027	optical and mechanical responses	996:1027	optical and mechanical responses of cells with different handednesses	996:1064	By simultaneously studying optical and mechanical responses of cells with different handednesses, we propose that the chirality of helicoids results from differences in cell wall composition.					
34911759	9	18	theme	right-handed	1529:1540	arg1	cells					1542:1546	right-handed cells	1529:1546	right-handed cells	1529:1546	Finally, 4) right-handed cells have an average lower elastic modulus compared to left-handed cells of the same color.					
34911759	0	19	theme	cellulose	67:75	arg1	architectures					77:89	helicoidal cellulose architectures	56:89	helicoidal cellulose architectures of Pollia condensata fruits	56:117	Cell wall composition determines handedness reversal in helicoidal cellulose architectures of Pollia condensata fruits.					
34911759	1	20	theme	wide	155:158	arg1	disciplines					171:181	disciplines	171:181	disciplines	171:181	Chiral asymmetry is important in a wide variety of disciplines and occurs across length scales.					
34911759	1	20	theme	wide	155:158	arg1	variety					160:166	a wide variety	153:166	a wide variety of disciplines	153:181	Chiral asymmetry is important in a wide variety of disciplines and occurs across length scales.					
34911759	8	21	theme	right-handed	1360:1371	arg1	cells					1373:1377	right-handed cells	1360:1377	right-handed cells	1360:1377	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	1	22	from	variety	160:166	arg1	important					140:148	important	140:148	important	140:148	Chiral asymmetry is important in a wide variety of disciplines and occurs across length scales.					
34911759	9	23	theme	average	1556:1562	arg1	modulus					1578:1584	an average lower elastic modulus	1553:1584	an average lower elastic modulus	1553:1584	Finally, 4) right-handed cells have an average lower elastic modulus compared to left-handed cells of the same color.					
34911759	0	24	theme	condensata	101:110	arg1	fruits					112:117	Pollia condensata fruits	94:117	Pollia condensata fruits	94:117	Cell wall composition determines handedness reversal in helicoidal cellulose architectures of Pollia condensata fruits.					
34911759	9	25	theme	elastic	1570:1576	arg1	modulus					1578:1584	an average lower elastic modulus	1553:1584	an average lower elastic modulus	1553:1584	Finally, 4) right-handed cells have an average lower elastic modulus compared to left-handed cells of the same color.					
34911759	1	26	from	important	140:148	arg1	disciplines					171:181	disciplines	171:181	disciplines	171:181	Chiral asymmetry is important in a wide variety of disciplines and occurs across length scales.					
34911759	1	26	from	important	140:148	arg1	variety					160:166	a wide variety	153:166	a wide variety of disciplines	153:181	Chiral asymmetry is important in a wide variety of disciplines and occurs across length scales.					
34911759	8	27	dep	shifted	1297:1303	arg1	1					1247:1247	1	1247:1247	1	1247:1247	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	8	28	theme	left-handed	1402:1412	arg1	ones					1414:1417	left-handed ones	1402:1417	left-handed ones	1402:1417	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	10	29	theme	mechanical	1663:1672	arg1	simulation					1674:1683	mechanical simulation	1663:1683	mechanical simulation	1663:1683	Our findings, combined with mechanical simulation, suggest that the different chiralities of helicoids in the cell wall may result from different chemical composition, which strengthens previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils.					
34911759	0	30	theme	Cell	0:3	arg1	composition					10:20	Cell wall composition	0:20	Cell wall composition	0:20	Cell wall composition determines handedness reversal in helicoidal cellulose architectures of Pollia condensata fruits.					
34911759	5	31	theme	cellulose	616:624	arg1	helicoids					626:634	These cellulose helicoids	610:634	These cellulose helicoids	610:634	These cellulose helicoids produce structural colors if their dimension is comparable to the wavelength of visible light.					
34911759	9	32	theme	left-handed	1598:1608	arg1	cells					1610:1614	left-handed cells	1598:1614	left-handed cells of the same color	1598:1632	Finally, 4) right-handed cells have an average lower elastic modulus compared to left-handed cells of the same color.					
34911759	10	33	theme	different	1703:1711	arg1	chiralities					1713:1723	the different chiralities	1699:1723	the different chiralities of helicoids in the cell wall	1699:1753	Our findings, combined with mechanical simulation, suggest that the different chiralities of helicoids in the cell wall may result from different chemical composition, which strengthens previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils.					
34911759	1	34	theme	Chiral	120:125	arg1	asymmetry					127:135	Chiral asymmetry	120:135	Chiral asymmetry	120:135	Chiral asymmetry is important in a wide variety of disciplines and occurs across length scales.					
34911759	3	35	theme	macroscopic	439:449	arg1	scales					451:456	the macroscopic scales	435:456	the macroscopic scales	435:456	Understanding how the handedness is transferred from molecular to the macroscopic scales is far from trivial.					
34911759	4	36	theme	cell	598:601	arg1	walls					603:607	plant cell walls	592:607	plant cell walls	592:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	10	37	theme	microfibrils	1901:1912	arg1	rotations					1878:1886	the rotations	1874:1886	the rotations of cellulose microfibrils	1874:1912	Our findings, combined with mechanical simulation, suggest that the different chiralities of helicoids in the cell wall may result from different chemical composition, which strengthens previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils.					
34911759	0	38	theme	handedness	33:42	arg1	reversal					44:51	handedness reversal	33:51	handedness reversal in helicoidal cellulose architectures of Pollia condensata fruits	33:117	Cell wall composition determines handedness reversal in helicoidal cellulose architectures of Pollia condensata fruits.					
34911759	6	39	theme	helicoidal	896:905	arg1	walls					912:916	both left- and right-handed helicoidal cell walls	868:916	both left- and right-handed helicoidal cell walls	868:916	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	9	40	theme	same	1623:1626	arg1	color					1628:1632	the same color	1619:1632	the same color	1619:1632	Finally, 4) right-handed cells have an average lower elastic modulus compared to left-handed cells of the same color.					
34911759	4	41	theme	organizations	549:561	arg1	handedness					524:533	the handedness	520:533	the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls	520:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	8	42	theme	right-handed	1427:1438	arg1	cells					1440:1444	right-handed cells	1427:1444	right-handed cells	1427:1444	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	4	43	from	organizations	549:561	arg1	walls					603:607	plant cell walls	592:607	plant cell walls	592:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	6	44	from	plants	793:798	arg1	examples					755:762	All previously reported examples	731:762	All previously reported examples of a helicoidal structure in plants	731:798	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	4	45	theme	cellulose	566:574	arg1	microfibrils					576:587	cellulose microfibrils	566:587	cellulose microfibrils in plant cell walls	566:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	9	46	contain	have	1548:1551	arg1	cells					1542:1546	right-handed cells	1529:1546	right-handed cells	1529:1546	Finally, 4) right-handed cells have an average lower elastic modulus compared to left-handed cells of the same color.					
34911759	9	46	contain	have	1548:1551	arg2	modulus					1578:1584	an average lower elastic modulus	1553:1584	an average lower elastic modulus	1553:1584	Finally, 4) right-handed cells have an average lower elastic modulus compared to left-handed cells of the same color.					
34911759	2	47	theme	opposite	347:354	arg1	chiralities					356:366	opposite chiralities	347:366	opposite chiralities	347:366	While several natural chiral biomolecules exist only with single handedness, they can produce complex hierarchical structures with opposite chiralities.					
34911759	6	48	theme	structure	780:788	arg1	examples					755:762	All previously reported examples	731:762	All previously reported examples of a helicoidal structure in plants	731:798	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	6	49	theme	same	956:959	arg1	tissue					961:966	the same tissue	952:966	the same tissue	952:966	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	8	50	from	regions	1468:1474	arg1	located					1450:1456	located	1450:1456	located	1450:1456	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	10	51	theme	cell	1745:1748	arg1	wall					1750:1753	the cell wall	1741:1753	the cell wall	1741:1753	Our findings, combined with mechanical simulation, suggest that the different chiralities of helicoids in the cell wall may result from different chemical composition, which strengthens previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils.					
34911759	6	52	from	structure	780:788	arg1	plants					793:798	plants	793:798	plants	793:798	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	6	53	theme	condensata	850:859	arg1	fruit					861:865	the Pollia condensata fruit	839:865	the Pollia condensata fruit	839:865	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	10	54	theme	chemical	1781:1788	arg1	composition					1790:1800	different chemical composition	1771:1800	different chemical composition	1771:1800	Our findings, combined with mechanical simulation, suggest that the different chiralities of helicoids in the cell wall may result from different chemical composition, which strengthens previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils.					
34911759	8	55	theme	statistical	1187:1197	arg1	substantiation					1199:1212	statistical substantiation	1187:1212	statistical substantiation of three different observations	1187:1244	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	6	56	theme	neighboring	931:941	arg1	cells					943:947	neighboring cells	931:947	neighboring cells of the same tissue	931:966	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	7	57	theme	cell	1138:1141	arg1	composition					1148:1158	cell wall composition	1138:1158	cell wall composition	1138:1158	By simultaneously studying optical and mechanical responses of cells with different handednesses, we propose that the chirality of helicoids results from differences in cell wall composition.					
34911759	1	58	theme	disciplines	171:181	arg1	disciplines					171:181	disciplines	171:181	disciplines	171:181	Chiral asymmetry is important in a wide variety of disciplines and occurs across length scales.					
34911759	1	58	theme	disciplines	171:181	arg1	variety					160:166	a wide variety	153:166	a wide variety of disciplines	153:181	Chiral asymmetry is important in a wide variety of disciplines and occurs across length scales.					
34911759	6	59	from	examples	755:762	arg1	plants					793:798	plants	793:798	plants	793:798	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	8	60	theme	right-handed	1271:1282	arg1	cells					1284:1288	right-handed cells	1271:1288	right-handed cells	1271:1288	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	8	61	theme	different	1223:1231	arg1	observations					1233:1244	three different observations	1217:1244	three different observations	1217:1244	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	6	62	located	found	922:926	arg2	walls					912:916	both left- and right-handed helicoidal cell walls	868:916	both left- and right-handed helicoidal cell walls	868:916	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	6	62	located	found	922:926	arg1	cells					943:947	neighboring cells	931:947	neighboring cells of the same tissue	931:966	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	2	63	theme	complex	310:316	arg1	structures					331:340	complex hierarchical structures	310:340	complex hierarchical structures	310:340	While several natural chiral biomolecules exist only with single handedness, they can produce complex hierarchical structures with opposite chiralities.					
34911759	8	64	from	located	1450:1456	arg1	regions					1468:1474	regions	1468:1474	regions corresponding to interlocular divisions	1468:1514	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	1	65	theme	length	201:206	arg1	scales					208:213	length scales	201:213	length scales	201:213	Chiral asymmetry is important in a wide variety of disciplines and occurs across length scales.					
34911759	8	66	dep	occur	1379:1383	arg1	2					1357:1357	2	1357:1357	2	1357:1357	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	5	67	theme	visible	716:722	arg1	light					724:728	visible light	716:728	visible light	716:728	These cellulose helicoids produce structural colors if their dimension is comparable to the wavelength of visible light.					
34911759	2	68	theme	natural	230:236	arg1	biomolecules					245:256	several natural chiral biomolecules	222:256	several natural chiral biomolecules	222:256	While several natural chiral biomolecules exist only with single handedness, they can produce complex hierarchical structures with opposite chiralities.					
34911759	2	69	theme	single	274:279	arg1	handedness					281:290	single handedness	274:290	single handedness	274:290	While several natural chiral biomolecules exist only with single handedness, they can produce complex hierarchical structures with opposite chiralities.					
34911759	5	70	theme	structural	644:653	arg1	colors					655:660	structural colors	644:660	structural colors	644:660	These cellulose helicoids produce structural colors if their dimension is comparable to the wavelength of visible light.					
34911759	8	71	theme	left-handed	1338:1348	arg1	cells					1350:1354	left-handed cells	1338:1354	left-handed cells	1338:1354	In detail, here we showed statistical substantiation of three different observations: 1) light reflected from right-handed cells is red shifted compared to light reflected from left-handed cells, 2) right-handed cells occur more rarely than left-handed ones, and 3) right-handed cells are located mainly in regions corresponding to interlocular divisions.					
34911759	7	72	with	cells	1032:1036	arg1	handednesses					1053:1064	different handednesses	1043:1064	different handednesses	1043:1064	By simultaneously studying optical and mechanical responses of cells with different handednesses, we propose that the chirality of helicoids results from differences in cell wall composition.					
34911759	4	73	theme	handedness	524:533	arg1	transfer					508:515	the transfer	504:515	the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls	504:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	4	73	theme	handedness	524:533	arg1	example					493:499	An intriguing example	479:499	An intriguing example	479:499	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	0	74	theme	Pollia	94:99	arg1	fruits					112:117	Pollia condensata fruits	94:117	Pollia condensata fruits	94:117	Cell wall composition determines handedness reversal in helicoidal cellulose architectures of Pollia condensata fruits.					
34911759	4	75	theme	helicoidal	538:547	arg1	organizations					549:561	helicoidal organizations	538:561	helicoidal organizations of cellulose microfibrils in plant cell walls	538:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	0	76	theme	fruits	112:117	arg1	architectures					77:89	helicoidal cellulose architectures	56:89	helicoidal cellulose architectures of Pollia condensata fruits	56:117	Cell wall composition determines handedness reversal in helicoidal cellulose architectures of Pollia condensata fruits.					
34911759	7	77	theme	mechanical	1008:1017	arg1	responses					1019:1027	optical and mechanical responses	996:1027	optical and mechanical responses of cells with different handednesses	996:1064	By simultaneously studying optical and mechanical responses of cells with different handednesses, we propose that the chirality of helicoids results from differences in cell wall composition.					
34911759	7	78	theme	optical	996:1002	arg1	responses					1019:1027	optical and mechanical responses	996:1027	optical and mechanical responses of cells with different handednesses	996:1064	By simultaneously studying optical and mechanical responses of cells with different handednesses, we propose that the chirality of helicoids results from differences in cell wall composition.					
34911759	0	79	from	reversal	44:51	arg1	architectures					77:89	helicoidal cellulose architectures	56:89	helicoidal cellulose architectures of Pollia condensata fruits	56:117	Cell wall composition determines handedness reversal in helicoidal cellulose architectures of Pollia condensata fruits.					
34911759	6	80	theme	reported	746:753	arg1	examples					755:762	All previously reported examples	731:762	All previously reported examples of a helicoidal structure in plants	731:798	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	4	81	from	walls	603:607	arg1	organizations					549:561	helicoidal organizations	538:561	helicoidal organizations of cellulose microfibrils in plant cell walls	538:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	7	82	from	differences	1123:1133	arg1	composition					1148:1158	cell wall composition	1138:1158	cell wall composition	1138:1158	By simultaneously studying optical and mechanical responses of cells with different handednesses, we propose that the chirality of helicoids results from differences in cell wall composition.					
34911759	9	83	dep	average	1556:1562	arg1	lower					1564:1568	lower	1564:1568	lower	1564:1568	Finally, 4) right-handed cells have an average lower elastic modulus compared to left-handed cells of the same color.					
34911759	9	84	dep	have	1548:1551	arg1	4					1526:1526	4	1526:1526	4	1526:1526	Finally, 4) right-handed cells have an average lower elastic modulus compared to left-handed cells of the same color.					
34911759	4	85	from	microfibrils	576:587	arg1	walls					603:607	plant cell walls	592:607	plant cell walls	592:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	6	86	theme	cell	907:910	arg1	walls					912:916	both left- and right-handed helicoidal cell walls	868:916	both left- and right-handed helicoidal cell walls	868:916	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	10	87	theme	cellulose	1891:1899	arg1	microfibrils					1901:1912	cellulose microfibrils	1891:1912	cellulose microfibrils	1891:1912	Our findings, combined with mechanical simulation, suggest that the different chiralities of helicoids in the cell wall may result from different chemical composition, which strengthens previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils.					
34911759	0	88	theme	helicoidal	56:65	arg1	architectures					77:89	helicoidal cellulose architectures	56:89	helicoidal cellulose architectures of Pollia condensata fruits	56:117	Cell wall composition determines handedness reversal in helicoidal cellulose architectures of Pollia condensata fruits.					
34911759	6	89	theme	right-handed	883:894	arg1	walls					912:916	both left- and right-handed helicoidal cell walls	868:916	both left- and right-handed helicoidal cell walls	868:916	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	9	90	theme	color	1628:1632	arg1	cells					1610:1614	left-handed cells	1598:1614	left-handed cells of the same color	1598:1632	Finally, 4) right-handed cells have an average lower elastic modulus compared to left-handed cells of the same color.					
34911759	4	91	theme	microfibrils	576:587	arg1	organizations					549:561	helicoidal organizations	538:561	helicoidal organizations of cellulose microfibrils in plant cell walls	538:607	An intriguing example is the transfer of the handedness of helicoidal organizations of cellulose microfibrils in plant cell walls.					
34911759	10	92	theme	helicoids	1728:1736	arg1	chiralities					1713:1723	the different chiralities	1699:1723	the different chiralities of helicoids in the cell wall	1699:1753	Our findings, combined with mechanical simulation, suggest that the different chiralities of helicoids in the cell wall may result from different chemical composition, which strengthens previous hypotheses that hemicellulose might mediate the rotations of cellulose microfibrils.					
34911759	6	93	theme	left-	873:877	arg1	walls					912:916	both left- and right-handed helicoidal cell walls	868:916	both left- and right-handed helicoidal cell walls	868:916	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	6	94	theme	helicoidal	769:778	arg1	structure					780:788	a helicoidal structure	767:788	a helicoidal structure in plants	767:798	All previously reported examples of a helicoidal structure in plants are left-handed except, remarkably, in the Pollia condensata fruit; both left- and right-handed helicoidal cell walls are found in neighboring cells of the same tissue.					
34911759	0	95	theme	wall	5:8	arg1	composition					10:20	Cell wall composition	0:20	Cell wall composition	0:20	Cell wall composition determines handedness reversal in helicoidal cellulose architectures of Pollia condensata fruits.					
34078541	0	0	theme	acid	60:63	arg1	profile					65:71	fatty acid profile	54:71	fatty acid profile	54:71	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	1	1	theme	cholesterol	266:276	arg1	COPs					298:301	COPs	298:301	COPs	298:301	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	1	theme	cholesterol	266:276	arg1	products					288:295	cholesterol oxidation products	266:295	cholesterol oxidation products (COPs)	266:302	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	4	2	theme	significant	763:773	arg1	decrease					775:782	significant decrease	763:782	significant decrease in cholesterol upto 6th month	763:812	During storage all products exhibited significant decrease in cholesterol upto 6th month, thereafter showed non-significant variation.					
34078541	7	3	from	increase	1063:1070	arg1	5-ch-3β-ol-7-one					1101:1116	5-ch-3β-ol-7-one	1101:1116	5-ch-3β-ol-7-one	1101:1116	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	7	3	from	increase	1063:1070	arg1	7-β-OH-ch					1075:1083	7-β-OH-ch	1075:1083	7-β-OH-ch	1075:1083	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	1	4	theme	canning	199:205	arg1	impact					171:176	the impact	167:176	the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs)	167:302	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	0	5	theme	fatty	54:58	arg1	profile					65:71	fatty acid profile	54:71	fatty acid profile	54:71	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	1	6	theme	oxidation	278:286	arg1	COPs					298:301	COPs	298:301	COPs	298:301	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	6	theme	oxidation	278:286	arg1	products					288:295	cholesterol oxidation products	266:295	cholesterol oxidation products (COPs)	266:302	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	2	7	theme	FAs	378:380	arg1	composition					382:392	The FAs composition	374:392	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage	374:497	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	7	8	theme	low-fat	1150:1156	arg1	goshtaba					1158:1165	low-fat goshtaba	1150:1165	low-fat goshtaba containing 1.5% XG	1150:1184	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	2	9	theme	saturated	399:407	arg1	FAs					409:411	saturated FAs	399:411	saturated FAs	399:411	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	2	10	theme	monounsaturated	414:428	arg1	FAs					430:432	monounsaturated FAs	414:432	monounsaturated FAs	414:432	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	1	11	theme	products	288:295	arg1	contents					240:247	fatty acids (FAs) contents	222:247	fatty acids (FAs) contents	222:247	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	11	theme	products	288:295	arg1	formation					253:261	formation	253:261	formation of cholesterol oxidation products (COPs)	253:302	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	12	theme	Kashmir	352:358	arg1	product					320:326	low-fat meat product	307:326	low-fat meat product (goshtaba) of Jammu and Kashmir (J&K)	307:364	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	12	theme	Kashmir	352:358	arg1	India					367:371	India	367:371	India	367:371	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	12	theme	Kashmir	352:358	arg1	goshtaba					329:336	goshtaba	329:336	goshtaba	329:336	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	7	13	theme	lower	1122:1126	arg1	COPs					1128:1131	lower COPs	1122:1131	lower COPs	1122:1131	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	1	14	theme	storage	211:217	arg1	impact					171:176	the impact	167:176	the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs)	167:302	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	4	15	dep	exhibited	753:761	arg1	showed					826:831	showed	826:831	thereafter showed non-significant variation	815:857	During storage all products exhibited significant decrease in cholesterol upto 6th month, thereafter showed non-significant variation.					
34078541	9	16	theme	cholesterol	1355:1365	arg1	content					1367:1373	cholesterol content	1355:1373	cholesterol content	1355:1373	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	2	17	theme	goshtaba	540:547	arg1	P > 0.05					559:566	P > 0.05	559:566	P > 0.05	559:566	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	2	17	theme	goshtaba	540:547	arg1	products					549:556	all goshtaba products	536:556	all goshtaba products (P > 0.05)	536:567	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	0	18	theme	cholesterol	77:87	arg1	oxidation					89:97	cholesterol oxidation	77:97	cholesterol oxidation of	77:100	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	6	19	dep	COPs	945:948	arg1	5-ch-3β-ol-7-one					962:977	5-ch-3β-ol-7-one	962:977	5-ch-3β-ol-7-one	962:977	After canning two COPs (7-β-OH-ch, 5-ch-3β-ol-7-one) were produced in all products.					
34078541	6	19	dep	COPs	945:948	arg1	7-β-OH-ch					951:959	7-β-OH-ch	951:959	7-β-OH-ch	951:959	After canning two COPs (7-β-OH-ch, 5-ch-3β-ol-7-one) were produced in all products.					
34078541	7	20	theme	25-OH-ch	1053:1060	arg1	formation					1040:1048	formation	1040:1048	formation of 25-OH-ch	1040:1060	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	7	20	theme	25-OH-ch	1053:1060	arg1	decrease					1089:1096	decrease	1089:1096	decrease in 5-ch-3β-ol-7-one	1089:1116	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	7	20	theme	25-OH-ch	1053:1060	arg1	increase					1063:1070	increase	1063:1070	increase in 7-β-OH-ch	1063:1083	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	9	21	from	variation	1397:1405	arg1	cholesterol					1432:1442	cholesterol	1432:1442	cholesterol	1432:1442	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	9	22	theme	lower	1461:1465	arg1	COPs					1467:1470	lower COPs	1461:1470	lower COPs	1461:1470	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	3	23	from	canning	624:630	arg1	P < 0.05					649:656	P < 0.05	649:656	P < 0.05	649:656	The cholesterol content decreased significantly after canning in all products (P < 0.05) while, maximum reduction was observed in high-fat goshtaba (HFC).					
34078541	3	23	from	canning	624:630	arg1	products					639:646	all products	635:646	all products (P < 0.05)	635:657	The cholesterol content decreased significantly after canning in all products (P < 0.05) while, maximum reduction was observed in high-fat goshtaba (HFC).					
34078541	8	24	contain	had	1256:1258	arg1	processing					1233:1242	processing	1233:1242	processing	1233:1242	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34078541	8	24	contain	had	1256:1258	arg1	replacer					1218:1225	fat replacer	1214:1225	fat replacer (XG)	1214:1230	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34078541	8	24	contain	had	1256:1258	arg1	XG					1228:1229	XG	1228:1229	XG	1228:1229	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34078541	8	24	contain	had	1256:1258	arg1	storage					1248:1254	storage	1248:1254	storage	1248:1254	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34078541	8	24	contain	had	1256:1258	arg2	effect					1275:1280	no significant effect	1260:1280	no significant effect	1260:1280	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34078541	9	25	theme	COPs	1467:1470	arg1	formation					1448:1456	formation	1448:1456	formation of lower COPs	1448:1470	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	4	26	theme	non-significant	833:847	arg1	variation					849:857	non-significant variation	833:857	non-significant variation	833:857	During storage all products exhibited significant decrease in cholesterol upto 6th month, thereafter showed non-significant variation.					
34078541	1	27	theme	fatty	222:226	arg1	FAs					235:237	FAs	235:237	FAs	235:237	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	27	theme	fatty	222:226	arg1	acids					228:232	fatty acids	222:232	fatty acids (FAs) contents	222:247	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	2	28	theme	non-significant	506:520	arg1	difference					522:531	non-significant difference	506:531	non-significant difference in all goshtaba products (P > 0.05)	506:567	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	7	29	contain	containing	1167:1176	arg1	goshtaba					1158:1165	low-fat goshtaba	1150:1165	low-fat goshtaba containing 1.5% XG	1150:1184	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	7	29	contain	containing	1167:1176	arg2	XG					1183:1184	1.5% XG	1178:1184	1.5% XG	1178:1184	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	8	30	theme	products	1322:1329	arg1	compositions					1293:1304	the FAs compositions	1285:1304	the FAs compositions of all goshtaba products including HFC	1285:1343	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34078541	0	31	theme	gum	19:21	arg1	Effects					0:6	Effects	0:6	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of	0:100	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	1	32	theme	acids	228:232	arg1	contents					240:247	fatty acids (FAs) contents	222:247	fatty acids (FAs) contents	222:247	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	2	33	dep	FAs	409:411	arg1	i.e.					394:397	i.e.	394:397	i.e.	394:397	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	9	34	from	reduction	1419:1427	arg1	cholesterol					1432:1442	cholesterol	1432:1442	cholesterol	1432:1442	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	8	35	theme	significant	1263:1273	arg1	effect					1275:1280	no significant effect	1260:1280	no significant effect	1260:1280	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34078541	0	36	theme	xanthan	11:17	arg1	gum					19:21	xanthan gum	11:21	xanthan gum	11:21	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	4	37	from	decrease	775:782	arg1	month					808:812	cholesterol upto 6th month	787:812	cholesterol upto 6th month	787:812	During storage all products exhibited significant decrease in cholesterol upto 6th month, thereafter showed non-significant variation.					
34078541	2	38	theme	polyunsaturated	435:449	arg1	FAs					451:453	polyunsaturated FAs	435:453	polyunsaturated FAs	435:453	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	1	39	theme	low-fat	307:313	arg1	product					320:326	low-fat meat product	307:326	low-fat meat product (goshtaba) of Jammu and Kashmir (J&K)	307:364	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	39	theme	low-fat	307:313	arg1	India					367:371	India	367:371	India	367:371	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	39	theme	low-fat	307:313	arg1	goshtaba					329:336	goshtaba	329:336	goshtaba	329:336	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	0	40	theme	canning	24:30	arg1	Effects					0:6	Effects	0:6	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of	0:100	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	9	41	theme	significant	1385:1395	arg1	variation					1397:1405	significant variation	1385:1405	significant variation	1385:1405	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	0	42	theme	meat	123:126	arg1	product					128:134	low-fat meat product	115:134	low-fat meat product of India	115:143	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	1	43	theme	meat	315:318	arg1	product					320:326	low-fat meat product	307:326	low-fat meat product (goshtaba) of Jammu and Kashmir (J&K)	307:364	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	43	theme	meat	315:318	arg1	India					367:371	India	367:371	India	367:371	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	43	theme	meat	315:318	arg1	goshtaba					329:336	goshtaba	329:336	goshtaba	329:336	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	9	44	theme	low-fat	1489:1495	arg1	goshtaba					1497:1504	low-fat goshtaba	1489:1504	low-fat goshtaba formulated with 1.5% XG	1489:1528	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	1	45	theme	xanthan	181:187	arg1	XG					194:195	XG	194:195	XG	194:195	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	45	theme	xanthan	181:187	arg1	gum					189:191	xanthan gum	181:191	xanthan gum (XG)	181:196	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	2	46	from	difference	522:531	arg1	P > 0.05					559:566	P > 0.05	559:566	P > 0.05	559:566	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	2	46	from	difference	522:531	arg1	products					549:556	all goshtaba products	536:556	all goshtaba products (P > 0.05)	536:567	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	0	47	theme	low-fat	115:121	arg1	product					128:134	low-fat meat product	115:134	low-fat meat product of India	115:143	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	1	48	theme	gum	189:191	arg1	impact					171:176	the impact	167:176	the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs)	167:302	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	0	49	theme	storage	36:42	arg1	period					44:49	storage period	36:49	storage period	36:49	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	4	50	theme	upto	799:802	arg1	month					808:812	cholesterol upto 6th month	787:812	cholesterol upto 6th month	787:812	During storage all products exhibited significant decrease in cholesterol upto 6th month, thereafter showed non-significant variation.					
34078541	8	51	theme	FAs	1289:1291	arg1	compositions					1293:1304	the FAs compositions	1285:1304	the FAs compositions of all goshtaba products including HFC	1285:1343	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34078541	0	52	from	Effects	0:6	arg1	profile					65:71	fatty acid profile	54:71	fatty acid profile	54:71	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	0	52	from	Effects	0:6	arg1	oxidation					89:97	cholesterol oxidation	77:97	cholesterol oxidation of	77:100	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	9	53	located	observed	1477:1484	arg1	goshtaba					1497:1504	low-fat goshtaba	1489:1504	low-fat goshtaba formulated with 1.5% XG	1489:1528	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	9	53	located	observed	1477:1484	arg2	formation					1448:1456	formation	1448:1456	formation of lower COPs	1448:1470	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	4	54	theme	cholesterol	787:797	arg1	month					808:812	cholesterol upto 6th month	787:812	cholesterol upto 6th month	787:812	During storage all products exhibited significant decrease in cholesterol upto 6th month, thereafter showed non-significant variation.					
34078541	1	55	from	impact	171:176	arg1	contents					240:247	fatty acids (FAs) contents	222:247	fatty acids (FAs) contents	222:247	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	55	from	impact	171:176	arg1	formation					253:261	formation	253:261	formation of cholesterol oxidation products (COPs)	253:302	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	3	56	located	observed	688:695	arg1	HFC					719:721	HFC	719:721	HFC	719:721	The cholesterol content decreased significantly after canning in all products (P < 0.05) while, maximum reduction was observed in high-fat goshtaba (HFC).					
34078541	3	56	located	observed	688:695	arg1	goshtaba					709:716	high-fat goshtaba	700:716	high-fat goshtaba (HFC)	700:722	The cholesterol content decreased significantly after canning in all products (P < 0.05) while, maximum reduction was observed in high-fat goshtaba (HFC).					
34078541	3	56	located	observed	688:695	arg2	reduction					674:682	maximum reduction	666:682	maximum reduction	666:682	The cholesterol content decreased significantly after canning in all products (P < 0.05) while, maximum reduction was observed in high-fat goshtaba (HFC).					
34078541	3	57	theme	maximum	666:672	arg1	reduction					674:682	maximum reduction	666:682	maximum reduction	666:682	The cholesterol content decreased significantly after canning in all products (P < 0.05) while, maximum reduction was observed in high-fat goshtaba (HFC).					
34078541	2	58	theme	trans	459:463	arg1	FAs					465:467	trans FAs	459:467	trans FAs	459:467	The FAs composition i.e. saturated FAs, monounsaturated FAs, polyunsaturated FAs and trans FAs during processing and storage showed non-significant difference in all goshtaba products (P > 0.05).					
34078541	3	59	theme	cholesterol	574:584	arg1	content					586:592	The cholesterol content	570:592	The cholesterol content	570:592	The cholesterol content decreased significantly after canning in all products (P < 0.05) while, maximum reduction was observed in high-fat goshtaba (HFC).					
34078541	9	60	theme	1.5	1522:1524	arg1	%					1525:1525	%	1525:1525	%	1525:1525	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	7	61	theme	1.5	1178:1180	arg1	%					1181:1181	%	1181:1181	%	1181:1181	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	3	62	theme	high-fat	700:707	arg1	HFC					719:721	HFC	719:721	HFC	719:721	The cholesterol content decreased significantly after canning in all products (P < 0.05) while, maximum reduction was observed in high-fat goshtaba (HFC).					
34078541	3	62	theme	high-fat	700:707	arg1	goshtaba					709:716	high-fat goshtaba	700:716	high-fat goshtaba (HFC)	700:722	The cholesterol content decreased significantly after canning in all products (P < 0.05) while, maximum reduction was observed in high-fat goshtaba (HFC).					
34078541	0	63	theme	India	139:143	arg1	product					128:134	low-fat meat product	115:134	low-fat meat product of India	115:143	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	9	64	theme	%	1525:1525	arg1	XG					1527:1528	1.5% XG	1522:1528	1.5% XG	1522:1528	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	7	65	located	observed	1138:1145	arg1	goshtaba					1158:1165	low-fat goshtaba	1150:1165	low-fat goshtaba containing 1.5% XG	1150:1184	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	7	65	located	observed	1138:1145	arg2	COPs					1128:1131	lower COPs	1122:1131	lower COPs	1122:1131	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	7	66	theme	%	1181:1181	arg1	XG					1183:1184	1.5% XG	1178:1184	1.5% XG	1178:1184	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	1	67	theme	Jammu	342:346	arg1	product					320:326	low-fat meat product	307:326	low-fat meat product (goshtaba) of Jammu and Kashmir (J&K)	307:364	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	67	theme	Jammu	342:346	arg1	India					367:371	India	367:371	India	367:371	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	1	67	theme	Jammu	342:346	arg1	goshtaba					329:336	goshtaba	329:336	goshtaba	329:336	This study evaluated the impact of xanthan gum (XG), canning and storage on fatty acids (FAs) contents and formation of cholesterol oxidation products (COPs) in low-fat meat product (goshtaba) of Jammu and Kashmir (J&K), India.					
34078541	8	68	theme	goshtaba	1313:1320	arg1	products					1322:1329	all goshtaba products	1309:1329	all goshtaba products including HFC	1309:1343	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34078541	9	69	theme	minimum	1411:1417	arg1	reduction					1419:1427	minimum reduction	1411:1427	minimum reduction	1411:1427	However, cholesterol content exhibited significant variation and minimum reduction in cholesterol and formation of lower COPs were observed in low-fat goshtaba formulated with 1.5% XG.					
34078541	0	70	theme	period	44:49	arg1	Effects					0:6	Effects	0:6	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of	0:100	Effects of xanthan gum, canning and storage period on fatty acid profile and cholesterol oxidation of restructured low-fat meat product of India.					
34078541	7	71	from	formation	1040:1048	arg1	5-ch-3β-ol-7-one					1101:1116	5-ch-3β-ol-7-one	1101:1116	5-ch-3β-ol-7-one	1101:1116	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	7	71	from	formation	1040:1048	arg1	7-β-OH-ch					1075:1083	7-β-OH-ch	1075:1083	7-β-OH-ch	1075:1083	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	7	72	from	decrease	1089:1096	arg1	5-ch-3β-ol-7-one					1101:1116	5-ch-3β-ol-7-one	1101:1116	5-ch-3β-ol-7-one	1101:1116	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	7	72	from	decrease	1089:1096	arg1	7-β-OH-ch					1075:1083	7-β-OH-ch	1075:1083	7-β-OH-ch	1075:1083	But during storage there was formation of 25-OH-ch, increase in 7-β-OH-ch and decrease in 5-ch-3β-ol-7-one and lower COPs were observed in low-fat goshtaba containing 1.5% XG.					
34078541	8	73	theme	fat	1214:1216	arg1	replacer					1218:1225	fat replacer	1214:1225	fat replacer (XG)	1214:1230	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34078541	8	73	theme	fat	1214:1216	arg1	XG					1228:1229	XG	1228:1229	XG	1228:1229	The results concluded that fat replacer (XG), processing and storage had no significant effect on the FAs compositions of all goshtaba products including HFC.					
34685527	10	0	theme	cell	1460:1463	arg1	type					1465:1468	a single cell type	1451:1468	a single cell type	1451:1468	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	6	1	theme	UGT80B1	923:929	arg1	mutants					912:918	mutants	912:918	mutants of UGT80B1	912:929	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	4	2	from	production	577:586	arg1	seeds					597:601	seeds	597:601	seeds	597:601	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	4	3	theme	Arabidopsis	497:507	arg1	UGT80A2					515:521	UGT80A2	515:521	UGT80A2	515:521	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	4	3	theme	Arabidopsis	497:507	arg1	SGTs					509:512	two Arabidopsis SGTs	493:512	two Arabidopsis SGTs	493:512	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	4	3	theme	Arabidopsis	497:507	arg1	UGT80B1/TT15					527:538	UGT80B1/TT15	527:538	UGT80B1/TT15	527:538	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	1	4	theme	sterol	166:171	arg1	SGTs					195:198	SGTs	195:198	SGTs	195:198	The conjugation of sterols with a Glc moiety is catalyzed by sterol glucosyltransferases (SGTs).					
34685527	1	4	theme	sterol	166:171	arg1	glucosyltransferases					173:192	sterol glucosyltransferases	166:192	sterol glucosyltransferases (SGTs)	166:199	The conjugation of sterols with a Glc moiety is catalyzed by sterol glucosyltransferases (SGTs).					
34685527	10	5	theme	Arabidopsis	1618:1628	arg1	seeds					1630:1634	Arabidopsis seeds	1618:1634	Arabidopsis seeds	1618:1634	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	4	6	theme	specialized	552:562	arg1	roles					564:568	specialized roles	552:568	specialized roles	552:568	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	6	7	theme	seed	836:839	arg1	mucilage					841:848	seed mucilage	836:848	seed mucilage	836:848	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	5	8	theme	coat	745:748	arg1	SCEs					767:770	SCEs	767:770	SCEs	767:770	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	5	8	theme	coat	745:748	arg1	cells					760:764	seed coat epidermal cells	740:764	seed coat epidermal cells (SCEs)	740:771	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	2	9	theme	long-chain	279:288	arg1	acid					296:299	a long-chain fatty acid	277:299	a long-chain fatty acid to form acyl-SG (ASG)	277:321	A portion of the resulting steryl glucosides (SG) are then esterified with a long-chain fatty acid to form acyl-SG (ASG).					
34685527	10	10	theme	SG	1511:1512	arg1	adjustment					1493:1502	the adjustment	1489:1502	the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1	1489:1577	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	9	11	theme	cell	1235:1238	arg1	defects					1245:1251	cell wall defects	1235:1251	cell wall defects	1235:1251	In contrast, cell wall defects were only observed in mutants defective for UGT80B1, while more mucilage cellulose was only observed when UGT80A2 was mutated.					
34685527	7	12	theme	additive	1035:1042	arg1	phenotype					1044:1052	an additive phenotype	1032:1052	an additive phenotype	1032:1052	In contrast, an additive phenotype was observed in double mutants for increased galactoglucomannan (GGM) content.					
34685527	10	13	theme	composition	1522:1532	arg1	adjustment					1493:1502	the adjustment	1489:1502	the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1	1489:1577	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	4	14	theme	Mutant	448:453	arg1	analysis					455:462	Mutant analysis	448:462	Mutant analysis	448:462	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	7	15	located	observed	1058:1065	arg1	contrast					1022:1029	contrast	1022:1029	contrast	1022:1029	In contrast, an additive phenotype was observed in double mutants for increased galactoglucomannan (GGM) content.					
34685527	7	15	located	observed	1058:1065	arg1	mutants					1077:1083	double mutants	1070:1083	double mutants for increased galactoglucomannan (GGM) content	1070:1130	In contrast, an additive phenotype was observed in double mutants for increased galactoglucomannan (GGM) content.					
34685527	7	15	located	observed	1058:1065	arg2	phenotype					1044:1052	an additive phenotype	1032:1052	an additive phenotype	1032:1052	In contrast, an additive phenotype was observed in double mutants for increased galactoglucomannan (GGM) content.					
34685527	9	16	theme	wall	1240:1243	arg1	defects					1245:1251	cell wall defects	1235:1251	cell wall defects	1235:1251	In contrast, cell wall defects were only observed in mutants defective for UGT80B1, while more mucilage cellulose was only observed when UGT80A2 was mutated.					
34685527	8	17	theme	polymer	1173:1179	arg1	density					1181:1187	increased polymer density	1163:1187	increased polymer density	1163:1187	Double mutants also exhibited increased polymer density within the inner mucilage layer.					
34685527	0	18	theme	Arabidopsis	66:76	arg1	Cells					98:102	Arabidopsis Seed Coat Epidermal Cells	66:102	Arabidopsis Seed Coat Epidermal Cells	66:102	Sterol Glucosyltransferases Tailor Polysaccharide Accumulation in Arabidopsis Seed Coat Epidermal Cells.					
34685527	6	19	theme	inner	821:825	arg1	layer					827:831	the inner layer	817:831	the inner layer of seed mucilage	817:848	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	7	20	theme	double	1070:1075	arg1	mutants					1077:1083	double mutants	1070:1083	double mutants for increased galactoglucomannan (GGM) content	1070:1130	In contrast, an additive phenotype was observed in double mutants for increased galactoglucomannan (GGM) content.					
34685527	10	21	theme	range	1400:1404	arg1	generation					1384:1393	The generation	1380:1393	The generation of a range of phenotypic effects	1380:1426	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	9	22	theme	more	1312:1315	arg1	cellulose					1326:1334	more mucilage cellulose	1312:1334	more mucilage cellulose	1312:1334	In contrast, cell wall defects were only observed in mutants defective for UGT80B1, while more mucilage cellulose was only observed when UGT80A2 was mutated.					
34685527	8	23	theme	increased	1163:1171	arg1	density					1181:1187	increased polymer density	1163:1187	increased polymer density	1163:1187	Double mutants also exhibited increased polymer density within the inner mucilage layer.					
34685527	3	24	theme	prevalent	339:347	arg1	ASG					331:333	ASG	331:333	ASG	331:333	SG and ASG are prevalent components of plant cellular membranes and influence their organization and functional properties.					
34685527	3	24	theme	prevalent	339:347	arg1	components					349:358	prevalent components	339:358	prevalent components of plant cellular membranes	339:386	SG and ASG are prevalent components of plant cellular membranes and influence their organization and functional properties.					
34685527	3	24	theme	prevalent	339:347	arg1	SG					324:325	SG	324:325	SG	324:325	SG and ASG are prevalent components of plant cellular membranes and influence their organization and functional properties.					
34685527	5	25	theme	polysaccharides	721:735	arg1	accumulation					705:716	the accumulation	701:716	the accumulation of polysaccharides in seed coat epidermal cells (SCEs)	701:771	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	6	26	theme	layer	827:831	arg1	content					806:812	The rhamnogalacturonan-I (RG-I) content	774:812	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage	774:848	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	6	26	theme	layer	827:831	arg1	higher					854:859	higher	854:859	higher	854:859	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	4	27	theme	enzymatic	632:640	arg1	activity					642:649	their enzymatic activity	626:649	their enzymatic activity	626:649	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	0	28	theme	Sterol	0:5	arg1	Glucosyltransferases					7:26	Sterol Glucosyltransferases	0:26	Sterol Glucosyltransferases	0:26	Sterol Glucosyltransferases Tailor Polysaccharide Accumulation in Arabidopsis Seed Coat Epidermal Cells.					
34685527	10	29	theme	ASG	1518:1520	arg1	composition					1522:1532	ASG composition	1518:1532	ASG composition	1518:1532	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	10	30	dep	SG	1511:1512	arg1	the					1507:1509	the	1507:1509	the	1507:1509	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	4	31	contain	have	547:550	arg1	UGT80A2					515:521	UGT80A2	515:521	UGT80A2	515:521	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	4	31	contain	have	547:550	arg1	SGTs					509:512	two Arabidopsis SGTs	493:512	two Arabidopsis SGTs	493:512	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	4	31	contain	have	547:550	arg1	UGT80B1/TT15					527:538	UGT80B1/TT15	527:538	UGT80B1/TT15	527:538	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	4	31	contain	have	547:550	arg2	roles					564:568	specialized roles	552:568	specialized roles	552:568	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34685527	10	32	theme	membranes	1546:1554	arg1	SG					1511:1512	SG	1511:1512	SG	1511:1512	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	10	32	theme	membranes	1546:1554	arg1	composition					1522:1532	ASG composition	1518:1532	ASG composition	1518:1532	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	0	33	theme	Polysaccharide	35:48	arg1	Accumulation					50:61	Polysaccharide Accumulation	35:61	Polysaccharide Accumulation	35:61	Sterol Glucosyltransferases Tailor Polysaccharide Accumulation in Arabidopsis Seed Coat Epidermal Cells.					
34685527	10	34	theme	effects	1420:1426	arg1	range					1400:1404	a range	1398:1404	a range of phenotypic effects	1398:1426	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	2	35	theme	fatty	290:294	arg1	acid					296:299	a long-chain fatty acid	277:299	a long-chain fatty acid to form acyl-SG (ASG)	277:321	A portion of the resulting steryl glucosides (SG) are then esterified with a long-chain fatty acid to form acyl-SG (ASG).					
34685527	8	36	theme	inner	1200:1204	arg1	layer					1215:1219	the inner mucilage layer	1196:1219	the inner mucilage layer	1196:1219	Double mutants also exhibited increased polymer density within the inner mucilage layer.					
34685527	1	37	with	conjugation	109:119	arg1	moiety					143:148	a Glc moiety	137:148	a Glc moiety	137:148	The conjugation of sterols with a Glc moiety is catalyzed by sterol glucosyltransferases (SGTs).					
34685527	10	38	theme	phenotypic	1409:1418	arg1	effects					1420:1426	phenotypic effects	1409:1426	phenotypic effects	1409:1426	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	1	39	theme	sterols	124:130	arg1	conjugation					109:119	The conjugation	105:119	The conjugation of sterols with a Glc moiety	105:148	The conjugation of sterols with a Glc moiety is catalyzed by sterol glucosyltransferases (SGTs).					
34685527	10	40	theme	polysaccharide	1587:1600	arg1	accumulation					1602:1613	polysaccharide accumulation	1587:1613	polysaccharide accumulation in Arabidopsis seeds	1587:1634	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	3	41	theme	cellular	369:376	arg1	membranes					378:386	plant cellular membranes	363:386	plant cellular membranes	363:386	SG and ASG are prevalent components of plant cellular membranes and influence their organization and functional properties.					
34685527	6	42	theme	RG-I	881:884	arg1	accumulation					886:897	RG-I accumulation	881:897	RG-I accumulation	881:897	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	5	43	theme	new	671:673	arg1	roles					675:679	new roles	671:679	new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs)	671:771	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	2	44	theme	glucosides	236:245	arg1	portion					204:210	A portion	202:210	A portion of the resulting steryl glucosides (SG)	202:250	A portion of the resulting steryl glucosides (SG) are then esterified with a long-chain fatty acid to form acyl-SG (ASG).					
34685527	3	45	theme	membranes	378:386	arg1	ASG					331:333	ASG	331:333	ASG	331:333	SG and ASG are prevalent components of plant cellular membranes and influence their organization and functional properties.					
34685527	3	45	theme	membranes	378:386	arg1	components					349:358	prevalent components	339:358	prevalent components of plant cellular membranes	339:386	SG and ASG are prevalent components of plant cellular membranes and influence their organization and functional properties.					
34685527	3	45	theme	membranes	378:386	arg1	SG					324:325	SG	324:325	SG	324:325	SG and ASG are prevalent components of plant cellular membranes and influence their organization and functional properties.					
34685527	9	46	theme	defective	1283:1291	arg1	mutants					1275:1281	mutants	1275:1281	mutants defective for UGT80B1	1275:1303	In contrast, cell wall defects were only observed in mutants defective for UGT80B1, while more mucilage cellulose was only observed when UGT80A2 was mutated.					
34685527	5	47	theme	seed	740:743	arg1	SCEs					767:770	SCEs	767:770	SCEs	767:770	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	5	47	theme	seed	740:743	arg1	cells					760:764	seed coat epidermal cells	740:764	seed coat epidermal cells (SCEs)	740:771	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	2	48	theme	steryl	229:234	arg1	SG					248:249	SG	248:249	SG	248:249	A portion of the resulting steryl glucosides (SG) are then esterified with a long-chain fatty acid to form acyl-SG (ASG).					
34685527	2	48	theme	steryl	229:234	arg1	glucosides					236:245	the resulting steryl glucosides	215:245	the resulting steryl glucosides (SG)	215:250	A portion of the resulting steryl glucosides (SG) are then esterified with a long-chain fatty acid to form acyl-SG (ASG).					
34685527	9	49	located	observed	1263:1270	arg1	contrast					1225:1232	contrast	1225:1232	contrast	1225:1232	In contrast, cell wall defects were only observed in mutants defective for UGT80B1, while more mucilage cellulose was only observed when UGT80A2 was mutated.					
34685527	9	49	located	observed	1263:1270	arg2	defects					1245:1251	cell wall defects	1235:1251	cell wall defects	1235:1251	In contrast, cell wall defects were only observed in mutants defective for UGT80B1, while more mucilage cellulose was only observed when UGT80A2 was mutated.					
34685527	9	49	located	observed	1263:1270	arg1	mutants					1275:1281	mutants	1275:1281	mutants defective for UGT80B1	1275:1303	In contrast, cell wall defects were only observed in mutants defective for UGT80B1, while more mucilage cellulose was only observed when UGT80A2 was mutated.					
34685527	3	50	theme	functional	425:434	arg1	properties					436:445	functional properties	425:445	functional properties	425:445	SG and ASG are prevalent components of plant cellular membranes and influence their organization and functional properties.					
34685527	0	51	theme	Seed	78:81	arg1	Cells					98:102	Arabidopsis Seed Coat Epidermal Cells	66:102	Arabidopsis Seed Coat Epidermal Cells	66:102	Sterol Glucosyltransferases Tailor Polysaccharide Accumulation in Arabidopsis Seed Coat Epidermal Cells.					
34685527	5	52	theme	epidermal	750:758	arg1	SCEs					767:770	SCEs	767:770	SCEs	767:770	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	5	52	theme	epidermal	750:758	arg1	cells					760:764	seed coat epidermal cells	740:764	seed coat epidermal cells (SCEs)	740:771	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	6	53	theme	RG-I	800:803	arg1	content					806:812	The rhamnogalacturonan-I (RG-I) content	774:812	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage	774:848	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	6	53	theme	RG-I	800:803	arg1	higher					854:859	higher	854:859	higher	854:859	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	2	54	theme	resulting	219:227	arg1	SG					248:249	SG	248:249	SG	248:249	A portion of the resulting steryl glucosides (SG) are then esterified with a long-chain fatty acid to form acyl-SG (ASG).					
34685527	2	54	theme	resulting	219:227	arg1	glucosides					236:245	the resulting steryl glucosides	215:245	the resulting steryl glucosides (SG)	215:250	A portion of the resulting steryl glucosides (SG) are then esterified with a long-chain fatty acid to form acyl-SG (ASG).					
34685527	3	55	theme	plant	363:367	arg1	membranes					378:386	plant cellular membranes	363:386	plant cellular membranes	363:386	SG and ASG are prevalent components of plant cellular membranes and influence their organization and functional properties.					
34685527	6	56	theme	mucilage	841:848	arg1	layer					827:831	the inner layer	817:831	the inner layer of seed mucilage	817:848	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	8	57	theme	Double	1133:1138	arg1	mutants					1140:1146	Double mutants	1133:1146	Double mutants	1133:1146	Double mutants also exhibited increased polymer density within the inner mucilage layer.					
34685527	10	58	theme	cellular	1537:1544	arg1	membranes					1546:1554	cellular membranes	1537:1554	cellular membranes	1537:1554	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	6	59	theme	rhamnogalacturonan-I	778:797	arg1	content					806:812	The rhamnogalacturonan-I (RG-I) content	774:812	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage	774:848	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	6	59	theme	rhamnogalacturonan-I	778:797	arg1	higher					854:859	higher	854:859	higher	854:859	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	1	60	theme	Glc	139:141	arg1	moiety					143:148	a Glc moiety	137:148	a Glc moiety	137:148	The conjugation of sterols with a Glc moiety is catalyzed by sterol glucosyltransferases (SGTs).					
34685527	6	61	theme	mutant	944:949	arg1	phenotypes					951:960	double mutant phenotypes	937:960	double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1	937:1016	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	5	62	from	roles	675:679	arg1	accumulation					705:716	the accumulation	701:716	the accumulation of polysaccharides in seed coat epidermal cells (SCEs)	701:771	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	6	63	theme	double	937:942	arg1	phenotypes					951:960	double mutant phenotypes	937:960	double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1	937:1016	The rhamnogalacturonan-I (RG-I) content of the inner layer of seed mucilage was higher in ugt80A2, whereas RG-I accumulation was lower in mutants of UGT80B1, with double mutant phenotypes indicating that UGT80A2 acts independently from UGT80B1.					
34685527	0	64	theme	Epidermal	88:96	arg1	Cells					98:102	Arabidopsis Seed Coat Epidermal Cells	66:102	Arabidopsis Seed Coat Epidermal Cells	66:102	Sterol Glucosyltransferases Tailor Polysaccharide Accumulation in Arabidopsis Seed Coat Epidermal Cells.					
34685527	5	65	from	accumulation	705:716	arg1	SCEs					767:770	SCEs	767:770	SCEs	767:770	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	5	65	from	accumulation	705:716	arg1	cells					760:764	seed coat epidermal cells	740:764	seed coat epidermal cells (SCEs)	740:771	Here, we establish new roles for both enzymes in the accumulation of polysaccharides in seed coat epidermal cells (SCEs).					
34685527	8	66	theme	mucilage	1206:1213	arg1	layer					1215:1219	the inner mucilage layer	1196:1219	the inner mucilage layer	1196:1219	Double mutants also exhibited increased polymer density within the inner mucilage layer.					
34685527	10	67	theme	single	1453:1458	arg1	type					1465:1468	a single cell type	1451:1468	a single cell type	1451:1468	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	9	68	theme	mucilage	1317:1324	arg1	cellulose					1326:1334	more mucilage cellulose	1312:1334	more mucilage cellulose	1312:1334	In contrast, cell wall defects were only observed in mutants defective for UGT80B1, while more mucilage cellulose was only observed when UGT80A2 was mutated.					
34685527	0	69	theme	Coat	83:86	arg1	Cells					98:102	Arabidopsis Seed Coat Epidermal Cells	66:102	Arabidopsis Seed Coat Epidermal Cells	66:102	Sterol Glucosyltransferases Tailor Polysaccharide Accumulation in Arabidopsis Seed Coat Epidermal Cells.					
34685527	7	70	theme	increased	1089:1097	arg1	content					1124:1130	increased galactoglucomannan (GGM) content	1089:1130	increased galactoglucomannan (GGM) content	1089:1130	In contrast, an additive phenotype was observed in double mutants for increased galactoglucomannan (GGM) content.					
34685527	10	71	from	accumulation	1602:1613	arg1	seeds					1630:1634	Arabidopsis seeds	1618:1634	Arabidopsis seeds	1618:1634	The generation of a range of phenotypic effects, simultaneously within a single cell type, demonstrates that the adjustment of the SG and ASG composition of cellular membranes by UGT80A2 and UGT80B1 tailors polysaccharide accumulation in Arabidopsis seeds.					
34685527	7	72	theme	galactoglucomannan	1099:1116	arg1	content					1124:1130	increased galactoglucomannan (GGM) content	1089:1130	increased galactoglucomannan (GGM) content	1089:1130	In contrast, an additive phenotype was observed in double mutants for increased galactoglucomannan (GGM) content.					
34685527	4	73	theme	SG	591:592	arg1	production					577:586	the production	573:586	the production of SG in seeds	573:601	Mutant analysis had previously inferred that two Arabidopsis SGTs, UGT80A2 and UGT80B1/TT15, could have specialized roles in the production of SG in seeds, despite an overlap in their enzymatic activity.					
34705842	14	0	theme	predictive	1528:1537	arg1	power					1539:1543	an excellent predictive power	1515:1543	an excellent predictive power (RMSEP = 1.82, RPD = 6.14)	1515:1570	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	12	1	theme	RMSEP	1267:1271	arg1	Bolaina					1258:1264	Bolaina	1258:1264	Bolaina (RMSEP = 0.81, RPD > 2)	1258:1288	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	12	1	theme	RMSEP	1267:1271	arg1	0.81					1275:1278	RMSEP = 0.81	1267:1278	RMSEP = 0.81	1267:1278	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	8	2	theme	chemical	639:646	arg1	composition					648:658	the chemical composition	635:658	the chemical composition using Fourier transform infrared (FTIR) spectra	635:706	The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra.					
34705842	13	3	theme	<	1411:1411	arg1	models					1371:1376	holocellulose models	1357:1376	holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1357:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	3	theme	<	1411:1411	arg1	1.96					1413:1416	0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96	1379:1416	1.96	1413:1416	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	5	4	theme	crinita	235:241	arg1	Bolaina					218:224	Bolaina	218:224	Bolaina (Guazuma crinita Lam.)	218:247	and Bolaina (Guazuma crinita Lam.)					
34705842	5	4	theme	crinita	235:241	arg1	Lam					243:245	Guazuma crinita Lam.	227:246	Guazuma crinita Lam.	227:246	and Bolaina (Guazuma crinita Lam.)					
34705842	11	5	theme	squares	1015:1021	arg1	models					1029:1034	build partial least squares (PLS) models	995:1034	build partial least squares (PLS) models from the FTIR spectra	995:1056	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	13	6	theme	<	1405:1405	arg1	models					1371:1376	holocellulose models	1357:1376	holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1357:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	6	theme	<	1405:1405	arg1	1.96					1413:1416	0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96	1379:1416	1.96	1413:1416	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	10	7	theme	significant	817:827	arg1	differences					829:839	significant differences	817:839	significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content	817:961	The results showed significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content.					
34705842	9	8	theme	FTIR-ATR	709:716	arg1	spectra					718:724	FTIR-ATR spectra	709:724	FTIR-ATR spectra	709:724	FTIR-ATR spectra were obtained from 150 samples for each species that were 1.8 year-old.					
34705842	12	9	dep	Capirona	1221:1228	arg1	>					1249:1249	>	1249:1249	>	1249:1249	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	10	10	theme	basal	864:868	arg1	stem					870:873	the apical and basal stem	849:873	the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content	849:961	The results showed significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content.					
34705842	13	11	theme	<	1384:1384	arg1	models					1371:1376	holocellulose models	1357:1376	holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1357:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	11	theme	<	1384:1384	arg1	1.96					1413:1416	0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96	1379:1416	1.96	1413:1416	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	12	theme	<	1392:1392	arg1	models					1371:1376	holocellulose models	1357:1376	holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1357:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	12	theme	<	1392:1392	arg1	1.96					1413:1416	0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96	1379:1416	1.96	1413:1416	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	14	13	theme	predictive	1637:1646	arg1	>					1659:1659	RPD > 2.0	1655:1663	RPD > 2.0	1655:1663	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	14	13	theme	predictive	1637:1646	arg1	power					1648:1652	a good predictive power	1630:1652	a good predictive power (RPD > 2.0)	1630:1664	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	11	14	theme	predictions	1112:1122	arg1	error					1103:1107	root mean squared error	1085:1107	root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD)	1085:1174	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	13	15	theme	holocellulose	1357:1369	arg1	models					1371:1376	holocellulose models	1357:1376	holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1357:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	15	theme	holocellulose	1357:1369	arg1	1.96					1413:1416	0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96	1379:1416	1.96	1413:1416	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	14	16	theme	good	1632:1635	arg1	>					1659:1659	RPD > 2.0	1655:1663	RPD > 2.0	1655:1663	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	14	16	theme	good	1632:1635	arg1	power					1648:1652	a good predictive power	1630:1652	a good predictive power (RPD > 2.0)	1630:1664	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	12	17	theme	=	1237:1237	arg1	0.48					1239:1242	RMSEP = 0.48	1231:1242	RMSEP = 0.48	1231:1242	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	3	18	dep	Capirona	159:166	arg1	Calycophyllum					169:181	Calycophyllum	169:181	Calycophyllum	169:181	Capirona (Calycophyllum spruceanum (Benth.)					
34705842	14	19	dep	power	1539:1543	arg1	=					1564:1564	=	1564:1564	=	1564:1564	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	0	20	theme	lignin	61:66	arg1	content					68:74	lignin content	61:74	lignin content	61:74	Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.)					
34705842	11	21	theme	partial	1001:1007	arg1	PLS					1024:1026	PLS	1024:1026	PLS	1024:1026	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	11	21	theme	partial	1001:1007	arg1	squares					1015:1021	partial least squares	1001:1021	build partial least squares (PLS) models from the FTIR spectra	995:1056	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	3	22	dep	Calycophyllum	169:181	arg1	spruceanum					183:192	Calycophyllum spruceanum	169:192	Calycophyllum spruceanum	169:192	Capirona (Calycophyllum spruceanum (Benth.)					
34705842	11	23	theme	performance	1145:1155	arg1	RMSEP					1125:1129	RMSEP	1125:1129	RMSEP	1125:1129	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	11	23	theme	performance	1145:1155	arg1	ratio					1136:1140	ratio	1136:1140	ratio of performance to deviation (RPD)	1136:1174	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	11	23	theme	performance	1145:1155	arg1	predictions					1112:1122	predictions	1112:1122	predictions (RMSEP)	1112:1130	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	8	24	theme	chemical	527:534	arg1	differences					536:546	chemical differences	527:546	chemical differences between apical and basal stem of Capirona and Bolaina	527:600	The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra.					
34705842	15	25	theme	juvenile	1784:1791	arg1	trees					1793:1797	juvenile trees	1784:1797	juvenile trees of Capirona and Bolaina	1784:1821	This study showed that FTIR-ATR together with PLS is a reliable method to determine the wood chemical composition in juvenile trees of Capirona and Bolaina.					
34705842	0	26	theme	in	87:88	arg1	Benth					116:120	Benth	116:120	Benth	116:120	Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.)					
34705842	0	26	theme	in	87:88	arg1	spruceanum					104:113	FTIR in Calycophyllum spruceanum	82:113	FTIR in Calycophyllum spruceanum (Benth.)	82:122	Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.)					
34705842	0	27	dep	in	87:88	arg1	Calycophyllum					90:102	Calycophyllum	90:102	Calycophyllum	90:102	Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.)					
34705842	13	28	from	Capirona	1294:1301	arg1	power					1319:1323	the predictive power	1304:1323	the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1304:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	28	from	Capirona	1294:1301	arg1	high					1424:1427	high	1424:1427	high	1424:1427	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	29	theme	models	1371:1376	arg1	power					1319:1323	the predictive power	1304:1323	the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1304:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	29	theme	models	1371:1376	arg1	high					1424:1427	high	1424:1427	high	1424:1427	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	15	30	theme	chemical	1760:1767	arg1	composition					1769:1779	the wood chemical composition	1751:1779	the wood chemical composition in juvenile trees of Capirona and Bolaina	1751:1821	This study showed that FTIR-ATR together with PLS is a reliable method to determine the wood chemical composition in juvenile trees of Capirona and Bolaina.					
34705842	11	31	from	spectra	1050:1056	arg1	models					1029:1034	build partial least squares (PLS) models	995:1034	build partial least squares (PLS) models from the FTIR spectra	995:1056	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	12	32	dep	>	1249:1249	arg1	0.48					1239:1242	RMSEP = 0.48	1231:1242	RMSEP = 0.48	1231:1242	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	0	33	theme	hemicellulose	17:29	arg1	Determination					0:12	Determination	0:12	Determination of hemicellulose, cellulose, holocellulose and lignin content	0:74	Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.)					
34705842	6	34	theme	fast-growing	253:264	arg1	trees					276:280	fast-growing Amazonian trees	253:280	fast-growing Amazonian trees	253:280	are fast-growing Amazonian trees with increasing demand in timber industry.					
34705842	13	35	theme	chemical	1452:1459	arg1	composition					1461:1471	wood chemical composition	1447:1471	wood chemical composition	1447:1471	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	36	theme	predictive	1308:1317	arg1	power					1319:1323	the predictive power	1304:1323	the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1304:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	36	theme	predictive	1308:1317	arg1	high					1424:1427	high	1424:1427	high	1424:1427	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	0	37	theme	cellulose	32:40	arg1	Determination					0:12	Determination	0:12	Determination of hemicellulose, cellulose, holocellulose and lignin content	0:74	Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.)					
34705842	10	38	theme	hemicellulose	915:927	arg1	terms					895:899	terms	895:899	terms of cellulose, hemicellulose, holocellulose and lignin content	895:961	The results showed significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content.					
34705842	12	39	theme	Lignin	1177:1182	arg1	content					1184:1190	Lignin content	1177:1190	Lignin content	1177:1190	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	2	40	theme	crinita	146:152	arg1	Lam					154:156	Guazuma crinita Lam	138:156	Guazuma crinita Lam	138:156	and Guazuma crinita Lam.					
34705842	0	41	theme	holocellulose	43:55	arg1	Determination					0:12	Determination	0:12	Determination of hemicellulose, cellulose, holocellulose and lignin content	0:74	Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.)					
34705842	10	42	theme	cellulose	904:912	arg1	terms					895:899	terms	895:899	terms of cellulose, hemicellulose, holocellulose and lignin content	895:961	The results showed significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content.					
34705842	15	43	theme	reliable	1722:1729	arg1	FTIR-ATR					1690:1697	FTIR-ATR	1690:1697	FTIR-ATR together with PLS	1690:1715	This study showed that FTIR-ATR together with PLS is a reliable method to determine the wood chemical composition in juvenile trees of Capirona and Bolaina.					
34705842	15	43	theme	reliable	1722:1729	arg1	method					1731:1736	a reliable method	1720:1736	a reliable method to determine the wood chemical composition in juvenile trees of Capirona and Bolaina	1720:1821	This study showed that FTIR-ATR together with PLS is a reliable method to determine the wood chemical composition in juvenile trees of Capirona and Bolaina.					
34705842	8	44	theme	Capirona	581:588	arg1	stem					573:576	apical and basal stem	556:576	apical and basal stem of Capirona and Bolaina	556:600	The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra.					
34705842	15	45	theme	wood	1755:1758	arg1	composition					1769:1779	the wood chemical composition	1751:1779	the wood chemical composition in juvenile trees of Capirona and Bolaina	1751:1821	This study showed that FTIR-ATR together with PLS is a reliable method to determine the wood chemical composition in juvenile trees of Capirona and Bolaina.					
34705842	10	46	theme	lignin	948:953	arg1	content					955:961	lignin content	948:961	lignin content	948:961	The results showed significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content.					
34705842	10	47	theme	apical	853:858	arg1	stem					870:873	the apical and basal stem	849:873	the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content	849:961	The results showed significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content.					
34705842	8	48	theme	Bolaina	594:600	arg1	stem					573:576	apical and basal stem	556:576	apical and basal stem of Capirona and Bolaina	556:600	The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra.					
34705842	12	49	dep	0.81	1275:1278	arg1	>					1285:1285	RPD > 2	1281:1287	RPD > 2	1281:1287	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	10	50	theme	holocellulose	930:942	arg1	terms					895:899	terms	895:899	terms of cellulose, hemicellulose, holocellulose and lignin content	895:961	The results showed significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content.					
34705842	10	51	from	species	884:890	arg1	terms					895:899	terms	895:899	terms of cellulose, hemicellulose, holocellulose and lignin content	895:961	The results showed significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content.					
34705842	13	52	theme	RPD	1407:1409	arg1	models					1371:1376	holocellulose models	1357:1376	holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1357:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	52	theme	RPD	1407:1409	arg1	1.96					1413:1416	0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96	1379:1416	1.96	1413:1416	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	14	53	theme	excellent	1518:1526	arg1	power					1539:1543	an excellent predictive power	1515:1543	an excellent predictive power (RMSEP = 1.82, RPD = 6.14)	1515:1570	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	12	54	theme	RPD	1281:1283	arg1	>					1285:1285	RPD > 2	1281:1287	RPD > 2	1281:1287	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	7	55	theme	juvenile	434:441	arg1	trees					443:447	juvenile trees	434:447	juvenile trees	434:447	Therefore, it is necessary to determine the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees to accelerate forest breeding programs.					
34705842	5	56	theme	Guazuma	227:233	arg1	Bolaina					218:224	Bolaina	218:224	Bolaina (Guazuma crinita Lam.)	218:247	and Bolaina (Guazuma crinita Lam.)					
34705842	5	56	theme	Guazuma	227:233	arg1	Lam					243:245	Guazuma crinita Lam.	227:246	Guazuma crinita Lam.	227:246	and Bolaina (Guazuma crinita Lam.)					
34705842	12	57	theme	=	1273:1273	arg1	Bolaina					1258:1264	Bolaina	1258:1264	Bolaina (RMSEP = 0.81, RPD > 2)	1258:1288	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	12	57	theme	=	1273:1273	arg1	0.81					1275:1278	RMSEP = 0.81	1267:1278	RMSEP = 0.81	1267:1278	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	11	58	theme	least	1009:1013	arg1	PLS					1024:1026	PLS	1024:1026	PLS	1024:1026	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	11	58	theme	least	1009:1013	arg1	squares					1015:1021	partial least squares	1001:1021	build partial least squares (PLS) models from the FTIR spectra	995:1056	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	7	59	theme	lignin	424:429	arg1	content					369:375	the content	365:375	the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees	365:447	Therefore, it is necessary to determine the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees to accelerate forest breeding programs.					
34705842	8	60	theme	infrared	684:691	arg1	spectra					700:706	infrared (FTIR) spectra	684:706	infrared (FTIR) spectra	684:706	The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra.					
34705842	14	61	theme	RPD	1655:1657	arg1	>					1659:1659	RPD > 2.0	1655:1663	RPD > 2.0	1655:1663	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	14	61	theme	RPD	1655:1657	arg1	power					1648:1652	a good predictive power	1630:1652	a good predictive power (RPD > 2.0)	1630:1664	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	13	62	theme	RMSEP	1386:1390	arg1	models					1371:1376	holocellulose models	1357:1376	holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1357:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	62	theme	RMSEP	1386:1390	arg1	1.96					1413:1416	0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96	1379:1416	1.96	1413:1416	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	7	63	theme	hemicellulose	391:403	arg1	content					369:375	the content	365:375	the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees	365:447	Therefore, it is necessary to determine the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees to accelerate forest breeding programs.					
34705842	7	64	theme	cellulose	380:388	arg1	content					369:375	the content	365:375	the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees	365:447	Therefore, it is necessary to determine the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees to accelerate forest breeding programs.					
34705842	15	65	theme	Capirona	1802:1809	arg1	trees					1793:1797	juvenile trees	1784:1797	juvenile trees of Capirona and Bolaina	1784:1821	This study showed that FTIR-ATR together with PLS is a reliable method to determine the wood chemical composition in juvenile trees of Capirona and Bolaina.					
34705842	15	66	theme	Bolaina	1815:1821	arg1	trees					1793:1797	juvenile trees	1784:1797	juvenile trees of Capirona and Bolaina	1784:1821	This study showed that FTIR-ATR together with PLS is a reliable method to determine the wood chemical composition in juvenile trees of Capirona and Bolaina.					
34705842	0	67	theme	content	68:74	arg1	Determination					0:12	Determination	0:12	Determination of hemicellulose, cellulose, holocellulose and lignin content	0:74	Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.)					
34705842	8	68	theme	apical	556:561	arg1	stem					573:576	apical and basal stem	556:576	apical and basal stem of Capirona and Bolaina	556:600	The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra.					
34705842	11	69	theme	build	995:999	arg1	models					1029:1034	build partial least squares (PLS) models	995:1034	build partial least squares (PLS) models from the FTIR spectra	995:1056	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	12	70	theme	RMSEP	1231:1235	arg1	0.48					1239:1242	RMSEP = 0.48	1231:1242	RMSEP = 0.48	1231:1242	Lignin content was efficiently predicted in Capirona (RMSEP = 0.48, RPD > 2) and Bolaina (RMSEP = 0.81, RPD > 2).					
34705842	0	71	theme	FTIR	82:85	arg1	Benth					116:120	Benth	116:120	Benth	116:120	Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.)					
34705842	0	71	theme	FTIR	82:85	arg1	spruceanum					104:113	FTIR in Calycophyllum spruceanum	82:113	FTIR in Calycophyllum spruceanum (Benth.)	82:122	Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.)					
34705842	11	72	theme	root	1085:1088	arg1	error					1103:1107	root mean squared error	1085:1107	root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD)	1085:1174	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	6	73	theme	timber	308:313	arg1	industry					315:322	timber industry	308:322	timber industry	308:322	are fast-growing Amazonian trees with increasing demand in timber industry.					
34705842	7	74	from	content	369:375	arg1	trees					443:447	juvenile trees	434:447	juvenile trees	434:447	Therefore, it is necessary to determine the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees to accelerate forest breeding programs.					
34705842	15	75	from	composition	1769:1779	arg1	trees					1793:1797	juvenile trees	1784:1797	juvenile trees of Capirona and Bolaina	1784:1821	This study showed that FTIR-ATR together with PLS is a reliable method to determine the wood chemical composition in juvenile trees of Capirona and Bolaina.					
34705842	11	76	theme	mean	1090:1093	arg1	error					1103:1107	root mean squared error	1085:1107	root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD)	1085:1174	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	13	77	theme	cellulose	1328:1336	arg1	power					1319:1323	the predictive power	1304:1323	the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1304:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	77	theme	cellulose	1328:1336	arg1	high					1424:1427	high	1424:1427	high	1424:1427	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	14	78	dep	=	1564:1564	arg1	RMSEP					1546:1550	RMSEP = 1.82	1546:1557	RMSEP = 1.82	1546:1557	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	13	79	theme	hemicellulose	1339:1351	arg1	power					1319:1323	the predictive power	1304:1323	the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96)	1304:1417	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	13	79	theme	hemicellulose	1339:1351	arg1	high					1424:1427	high	1424:1427	high	1424:1427	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	6	80	theme	Amazonian	266:274	arg1	trees					276:280	fast-growing Amazonian trees	253:280	fast-growing Amazonian trees	253:280	are fast-growing Amazonian trees with increasing demand in timber industry.					
34705842	13	81	theme	wood	1447:1450	arg1	composition					1461:1471	wood chemical composition	1447:1471	wood chemical composition	1447:1471	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
34705842	8	82	theme	basal	567:571	arg1	stem					573:576	apical and basal stem	556:576	apical and basal stem of Capirona and Bolaina	556:600	The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra.					
34705842	7	83	theme	holocellulose	406:418	arg1	content					369:375	the content	365:375	the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees	365:447	Therefore, it is necessary to determine the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees to accelerate forest breeding programs.					
34705842	11	84	theme	ratio	1136:1140	arg1	error					1103:1107	root mean squared error	1085:1107	root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD)	1085:1174	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	11	85	theme	FTIR	1045:1048	arg1	spectra					1050:1056	the FTIR spectra	1041:1056	the FTIR spectra	1041:1056	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	7	86	theme	breeding	470:477	arg1	programs					479:486	forest breeding programs	463:486	forest breeding programs	463:486	Therefore, it is necessary to determine the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees to accelerate forest breeding programs.					
34705842	8	87	dep	infrared	684:691	arg1	FTIR					694:697	FTIR	694:697	FTIR	694:697	The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra.					
34705842	10	88	theme	content	955:961	arg1	terms					895:899	terms	895:899	terms of cellulose, hemicellulose, holocellulose and lignin content	895:961	The results showed significant differences between the apical and basal stem for each species in terms of cellulose, hemicellulose, holocellulose and lignin content.					
34705842	8	89	theme	study	505:509	arg1	aim					493:495	The aim	489:495	The aim of this study	489:509	The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra.					
34705842	11	90	theme	squared	1095:1101	arg1	error					1103:1107	root mean squared error	1085:1107	root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD)	1085:1174	This variability was useful to build partial least squares (PLS) models from the FTIR spectra and they were evaluated by root mean squared error of predictions (RMSEP) and ratio of performance to deviation (RPD).					
34705842	14	91	theme	=	1552:1552	arg1	RMSEP					1546:1550	RMSEP = 1.82	1546:1557	RMSEP = 1.82	1546:1557	In Bolaina, model for cellulose attained an excellent predictive power (RMSEP = 1.82, RPD = 6.14) while models for hemicellulose and holocellulose attained a good predictive power (RPD > 2.0).					
34705842	8	92	dep	Fourier	666:672	arg1	transform					674:682	transform	674:682	transform infrared (FTIR) spectra	674:706	The aim of this study was to identify chemical differences between apical and basal stem of Capirona and Bolaina to develop models for estimating the chemical composition using Fourier transform infrared (FTIR) spectra.					
34705842	7	93	theme	forest	463:468	arg1	programs					479:486	forest breeding programs	463:486	forest breeding programs	463:486	Therefore, it is necessary to determine the content of cellulose, hemicellulose, holocellulose and lignin in juvenile trees to accelerate forest breeding programs.					
34705842	2	94	theme	Guazuma	138:144	arg1	Lam					154:156	Guazuma crinita Lam	138:156	Guazuma crinita Lam	138:156	and Guazuma crinita Lam.					
34705842	13	95	from	high	1424:1427	arg1	Capirona					1294:1301	Capirona	1294:1301	Capirona	1294:1301	In Capirona, the predictive power of cellulose, hemicellulose and holocellulose models (0.68 < RMSEP < 2.06, 1.60 < RPD < 1.96) were high enough to predict wood chemical composition.					
32497941	8	0	theme	protocols	1157:1165	arg1	selection					1132:1140	selection	1132:1140	selection of degradation protocols based on their ability to degrade chitin and chitosan and facilitate the production of COS with desired compositions	1132:1282	Additionally, our results enable selection of degradation protocols based on their ability to degrade chitin and chitosan and facilitate the production of COS with desired compositions.					
32497941	6	1	theme	GlcN	855:858	arg1	monomers					896:903	GlcN and N-acetyl-d-glucosamine (GlcNAc) monomers	855:903	GlcN and N-acetyl-d-glucosamine (GlcNAc) monomers	855:903	Hydrogen peroxide degrades chitosan to produce GlcN and N-acetyl-d-glucosamine (GlcNAc) monomers and oligomers, and nitrous acid degrades chitosan to produce 2,5-anhydro- d-mannose.					
32497941	1	2	theme	chitosan	181:188	arg1	COS					208:210	COS	208:210	COS	208:210	The beneficial effects provided by chitosan oligosaccharides (COS) make them of interest in medical research.					
32497941	1	2	theme	chitosan	181:188	arg1	oligosaccharides					190:205	chitosan oligosaccharides	181:205	chitosan oligosaccharides (COS)	181:211	The beneficial effects provided by chitosan oligosaccharides (COS) make them of interest in medical research.					
32497941	4	3	theme	hydrochloric	599:610	arg1	acid					612:615	hydrochloric acid	599:615	hydrochloric acid	599:615	Our results show that hydrochloric acid, hydrogen peroxide, and nitrous acid generate distinct products from chitin and chitosan.					
32497941	0	4	theme	ionization	101:110	arg1	spectrometry					132:143	electrospray ionization time-of-flight mass spectrometry	88:143	electrospray ionization time-of-flight mass spectrometry	88:143	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	0	5	from	Identification	0:13	arg1	chitosan					76:83	chitosan	76:83	chitosan	76:83	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	0	5	from	Identification	0:13	arg1	chitin					65:70	chitin	65:70	chitin	65:70	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	7	6	theme	COS	1012:1014	arg1	composition					1016:1026	COS composition	1012:1026	COS composition	1012:1026	Our studies show that COS composition is dictated by both the degradation protocol and the starting polymer.					
32497941	4	7	theme	hydrogen	618:625	arg1	peroxide					627:634	hydrogen peroxide	618:634	hydrogen peroxide	618:634	Our results show that hydrochloric acid, hydrogen peroxide, and nitrous acid generate distinct products from chitin and chitosan.					
32497941	0	8	theme	electrospray	88:99	arg1	spectrometry					132:143	electrospray ionization time-of-flight mass spectrometry	88:143	electrospray ionization time-of-flight mass spectrometry	88:143	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	2	9	theme	distinct	296:303	arg1	properties					305:314	distinct properties	296:314	distinct properties	296:314	The monomers that constitute COS confer distinct properties, so controlling COS composition during their production is significant.					
32497941	4	10	from	chitosan	697:704	arg1	products					672:679	distinct products	663:679	distinct products from chitin and chitosan	663:704	Our results show that hydrochloric acid, hydrogen peroxide, and nitrous acid generate distinct products from chitin and chitosan.					
32497941	8	11	theme	desired	1263:1269	arg1	compositions					1271:1282	desired compositions	1263:1282	desired compositions	1263:1282	Additionally, our results enable selection of degradation protocols based on their ability to degrade chitin and chitosan and facilitate the production of COS with desired compositions.					
32497941	0	12	theme	mass	127:130	arg1	spectrometry					132:143	electrospray ionization time-of-flight mass spectrometry	88:143	electrospray ionization time-of-flight mass spectrometry	88:143	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	6	13	theme	nitrous	924:930	arg1	acid					932:935	nitrous acid	924:935	nitrous acid	924:935	Hydrogen peroxide degrades chitosan to produce GlcN and N-acetyl-d-glucosamine (GlcNAc) monomers and oligomers, and nitrous acid degrades chitosan to produce 2,5-anhydro- d-mannose.					
32497941	3	14	theme	time-of-flight	543:556	arg1	spectrometry					563:574	electrospray ionization time-of-flight mass spectrometry	519:574	electrospray ionization time-of-flight mass spectrometry	519:574	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	3	15	theme	identified	447:456	arg1	products					479:486	identified low molecular weight products	447:486	identified low molecular weight products	447:486	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	3	15	theme	identified	447:456	arg1	COS					496:498	COS	496:498	COS	496:498	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	0	16	theme	time-of-flight	112:125	arg1	spectrometry					132:143	electrospray ionization time-of-flight mass spectrometry	88:143	electrospray ionization time-of-flight mass spectrometry	88:143	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	3	17	theme	mass	558:561	arg1	spectrometry					563:574	electrospray ionization time-of-flight mass spectrometry	519:574	electrospray ionization time-of-flight mass spectrometry	519:574	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	4	18	from	chitin	686:691	arg1	products					672:679	distinct products	663:679	distinct products from chitin and chitosan	663:704	Our results show that hydrochloric acid, hydrogen peroxide, and nitrous acid generate distinct products from chitin and chitosan.					
32497941	3	19	theme	low	458:460	arg1	weight					472:477	low molecular weight	458:477	identified low molecular weight products	447:486	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	2	20	theme	COS	332:334	arg1	composition					336:346	COS composition	332:346	COS composition	332:346	The monomers that constitute COS confer distinct properties, so controlling COS composition during their production is significant.					
32497941	8	21	theme	degradation	1145:1155	arg1	protocols					1157:1165	degradation protocols	1145:1165	degradation protocols based on their ability to degrade chitin and chitosan and facilitate the production of COS with desired compositions	1145:1282	Additionally, our results enable selection of degradation protocols based on their ability to degrade chitin and chitosan and facilitate the production of COS with desired compositions.					
32497941	6	22	theme	Hydrogen	808:815	arg1	peroxide					817:824	Hydrogen peroxide	808:824	Hydrogen peroxide	808:824	Hydrogen peroxide degrades chitosan to produce GlcN and N-acetyl-d-glucosamine (GlcNAc) monomers and oligomers, and nitrous acid degrades chitosan to produce 2,5-anhydro- d-mannose.					
32497941	0	23	theme	products	51:58	arg1	Identification					0:13	Identification	0:13	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.	0:144	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	0	24	theme	molecular	22:30	arg1	products					51:58	low molecular weight degradation products	18:58	low molecular weight degradation products from chitin and chitosan	18:83	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	0	25	theme	low	18:20	arg1	products					51:58	low molecular weight degradation products	18:58	low molecular weight degradation products from chitin and chitosan	18:83	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	0	26	theme	degradation	39:49	arg1	products					51:58	low molecular weight degradation products	18:58	low molecular weight degradation products from chitin and chitosan	18:83	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	0	27	from	chitosan	76:83	arg1	Identification					0:13	Identification	0:13	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.	0:144	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	0	27	from	chitosan	76:83	arg1	products					51:58	low molecular weight degradation products	18:58	low molecular weight degradation products from chitin and chitosan	18:83	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	3	28	theme	molecular	462:470	arg1	weight					472:477	low molecular weight	458:477	identified low molecular weight products	447:486	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	0	29	theme	weight	32:37	arg1	products					51:58	low molecular weight degradation products	18:58	low molecular weight degradation products from chitin and chitosan	18:83	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	3	30	theme	chitosan	425:432	arg1	polymers					434:441	chitin and chitosan polymers	414:441	chitin and chitosan polymers	414:441	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	3	30	theme	chitosan	425:432	arg1	COS					496:498	COS	496:498	COS	496:498	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	7	31	theme	degradation	1052:1062	arg1	protocol					1064:1071	the degradation protocol	1048:1071	the degradation protocol	1048:1071	Our studies show that COS composition is dictated by both the degradation protocol and the starting polymer.					
32497941	3	32	theme	weight	472:477	arg1	products					479:486	identified low molecular weight products	447:486	identified low molecular weight products	447:486	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	3	32	theme	weight	472:477	arg1	COS					496:498	COS	496:498	COS	496:498	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	7	33	theme	starting	1081:1088	arg1	polymer					1090:1096	the starting polymer	1077:1096	the starting polymer	1077:1096	Our studies show that COS composition is dictated by both the degradation protocol and the starting polymer.					
32497941	0	34	from	chitin	65:70	arg1	Identification					0:13	Identification	0:13	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.	0:144	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	0	34	from	chitin	65:70	arg1	products					51:58	low molecular weight degradation products	18:58	low molecular weight degradation products from chitin and chitosan	18:83	Identification of low molecular weight degradation products from chitin and chitosan by electrospray ionization time-of-flight mass spectrometry.					
32497941	8	35	with	production	1240:1249	arg1	compositions					1271:1282	desired compositions	1263:1282	desired compositions	1263:1282	Additionally, our results enable selection of degradation protocols based on their ability to degrade chitin and chitosan and facilitate the production of COS with desired compositions.					
32497941	3	36	theme	chitin	414:419	arg1	polymers					434:441	chitin and chitosan polymers	414:441	chitin and chitosan polymers	414:441	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	3	36	theme	chitin	414:419	arg1	COS					496:498	COS	496:498	COS	496:498	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	5	37	theme	Hydrochloric	707:718	arg1	acid					720:723	Hydrochloric acid	707:723	Hydrochloric acid	707:723	Hydrochloric acid degrades chitin and chitosan to produce glucosamine (GlcN) monomers and oligomers.					
32497941	8	38	theme	COS	1254:1256	arg1	production					1240:1249	the production	1236:1249	the production of COS with desired compositions	1236:1282	Additionally, our results enable selection of degradation protocols based on their ability to degrade chitin and chitosan and facilitate the production of COS with desired compositions.					
32497941	6	39	theme	2,5-anhydro-	966:977	arg1	d-mannose					979:987	2,5-anhydro- d-mannose	966:987	2,5-anhydro- d-mannose	966:987	Hydrogen peroxide degrades chitosan to produce GlcN and N-acetyl-d-glucosamine (GlcNAc) monomers and oligomers, and nitrous acid degrades chitosan to produce 2,5-anhydro- d-mannose.					
32497941	6	40	theme	N-acetyl-d-glucosamine	864:885	arg1	monomers					896:903	GlcN and N-acetyl-d-glucosamine (GlcNAc) monomers	855:903	GlcN and N-acetyl-d-glucosamine (GlcNAc) monomers	855:903	Hydrogen peroxide degrades chitosan to produce GlcN and N-acetyl-d-glucosamine (GlcNAc) monomers and oligomers, and nitrous acid degrades chitosan to produce 2,5-anhydro- d-mannose.					
32497941	1	41	theme	beneficial	150:159	arg1	effects					161:167	The beneficial effects	146:167	The beneficial effects provided by chitosan oligosaccharides (COS)	146:211	The beneficial effects provided by chitosan oligosaccharides (COS) make them of interest in medical research.					
32497941	1	42	theme	medical	238:244	arg1	research					246:253	medical research	238:253	medical research	238:253	The beneficial effects provided by chitosan oligosaccharides (COS) make them of interest in medical research.					
32497941	4	43	theme	distinct	663:670	arg1	products					672:679	distinct products	663:679	distinct products from chitin and chitosan	663:704	Our results show that hydrochloric acid, hydrogen peroxide, and nitrous acid generate distinct products from chitin and chitosan.					
32497941	3	44	theme	electrospray	519:530	arg1	spectrometry					563:574	electrospray ionization time-of-flight mass spectrometry	519:574	electrospray ionization time-of-flight mass spectrometry	519:574	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	4	45	theme	nitrous	641:647	arg1	acid					649:652	nitrous acid	641:652	nitrous acid	641:652	Our results show that hydrochloric acid, hydrogen peroxide, and nitrous acid generate distinct products from chitin and chitosan.					
32497941	3	46	theme	ionization	532:541	arg1	spectrometry					563:574	electrospray ionization time-of-flight mass spectrometry	519:574	electrospray ionization time-of-flight mass spectrometry	519:574	In this work, we degraded chitin and chitosan polymers and identified low molecular weight products such as COS that formed, using electrospray ionization time-of-flight mass spectrometry.					
32497941	5	47	theme	glucosamine	765:775	arg1	monomers					784:791	glucosamine (GlcN) monomers	765:791	glucosamine (GlcN) monomers	765:791	Hydrochloric acid degrades chitin and chitosan to produce glucosamine (GlcN) monomers and oligomers.					
34086701	8	0	theme	acetyl	1685:1690	arg1	esterase					1698:1705	acetyl xylan esterase	1685:1705	acetyl xylan esterase	1685:1705	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	3	1	theme	lignin	470:475	arg1	matrix					422:427	a highly interconnected matrix	398:427	a highly interconnected matrix of various polysaccharides, proteins and lignin	398:475	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	3	1	theme	lignin	470:475	arg1	wall					390:393	The cell wall	381:393	The cell wall	381:393	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	9	2	dep	wheat	2147:2151	arg1	oat					2170:2172	oat	2170:2172	oat	2170:2172	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	9	2	dep	wheat	2147:2151	arg1	rye					2162:2164	rye	2162:2164	rye	2162:2164	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	9	2	dep	wheat	2147:2151	arg1	barley					2154:2159	barley	2154:2159	barley	2154:2159	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	8	3	with	Combination	1582:1592	arg1	xylanases					1774:1782	both GH10 and GH11 xylanases	1755:1782	xylanases	1774:1782	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	6	4	theme	non-starch	1127:1136	arg1	analysis					1163:1170	non-starch polysaccharide component analysis	1127:1170	non-starch polysaccharide component analysis	1127:1170	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	10	5	contain	contained	2218:2226	arg1	secretome					2195:2203	the secretome	2191:2203	the secretome of T. reesei	2191:2216	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	10	5	contain	contained	2218:2226	arg2	amounts					2240:2246	significant amounts	2228:2246	significant amounts	2228:2246	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	10	5	contain	contained	2218:2226	arg2	enzyme					2287:2292	an exceptional direct chain-cutting enzyme	2251:2292	an exceptional direct chain-cutting enzyme from the GH74 family	2251:2313	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	9	6	dep	β-glucanases	1906:1917	arg1	endo-β-1,3-glucanase					1953:1972	endo-β-1,3-glucanase	1953:1972	endo-β-1,3-glucanase	1953:1972	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	9	6	dep	β-glucanases	1906:1917	arg1	-glucanase					1938:1947	-glucanase	1938:1947	-glucanase	1938:1947	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	1	7	theme	feed	240:243	arg1	industry					245:252	the animal feed industry	229:252	the animal feed industry	229:252	A diverse range of monocot and dicot grains and their by-products are commonly used in the animal feed industry.					
34086701	4	8	from	potential	601:609	arg1	fermentation					680:691	a wild type fermentation	668:691	a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain)	668:748	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	7	9	theme	T.	1466:1467	arg1	fermentation					1476:1487	The T. reesei fermentation	1462:1487	The T. reesei fermentation	1462:1487	The T. reesei fermentation also decreased viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates.					
34086701	6	10	theme	in	1102:1103	arg1	incubations					1111:1121	in vitro incubations	1102:1121	in vitro incubations	1102:1121	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	9	11	theme	T.	2014:2015	arg1	fermentation					2024:2035	the T. reesei fermentation	2010:2035	the T. reesei fermentation	2010:2035	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	6	12	dep	in	1102:1103	arg1	vitro					1105:1109	vitro	1105:1109	vitro	1105:1109	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	2	13	theme	complex	274:280	arg1	structures					305:314	complex and variable cell wall structures	274:314	complex and variable cell wall structures which in turn contribute significant fiber to the complete feed	274:378	They all come with complex and variable cell wall structures which in turn contribute significant fiber to the complete feed.					
34086701	11	14	theme	present	2490:2496	arg1	enzymes					2482:2488	enzymes	2482:2488	enzymes present in the T. reesei secretome	2482:2523	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	0	15	theme	plant	109:113	arg1	substrates					115:124	plant substrates	109:124	plant substrates	109:124	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	3	16	theme	collaborative	502:514	arg1	effort					516:521	a collaborative effort	500:521	a collaborative effort of different enzymes for its degradation	500:562	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	1	17	theme	grains	179:184	arg1	range					152:156	A diverse range	142:156	A diverse range of monocot and dicot grains	142:184	A diverse range of monocot and dicot grains and their by-products are commonly used in the animal feed industry.					
34086701	1	17	theme	grains	179:184	arg1	by-products					196:206	their by-products	190:206	their by-products	190:206	A diverse range of monocot and dicot grains and their by-products are commonly used in the animal feed industry.					
34086701	11	18	attach	present	2490:2496	arg2	enzymes					2482:2488	enzymes	2482:2488	enzymes present in the T. reesei secretome	2482:2523	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	11	18	attach	present	2490:2496	arg1	secretome					2515:2523	the T. reesei secretome	2501:2523	the T. reesei secretome	2501:2523	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	6	19	theme	carbohydrase	1302:1313	arg1	product					1315:1321	the carbohydrase product	1298:1321	the carbohydrase product	1298:1321	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	5	20	theme	mass	1002:1005	arg1	LC-MS/MS					1020:1027	tandem mass spectrometry LC-MS/MS	995:1027	tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases	995:1084	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	5	20	theme	mass	1002:1005	arg1	hydrolases					1048:1057	glycosyl hydrolases	1039:1057	glycosyl hydrolases	1039:1057	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	5	20	theme	mass	1002:1005	arg1	esterases					1076:1084	carbohydrate esterases	1063:1084	carbohydrate esterases	1063:1084	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	4	21	theme	rice	816:819	arg1	bran					821:824	de-oiled rice bran	807:824	de-oiled rice bran	807:824	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	4	21	theme	rice	816:819	arg1	barley					794:799	barley	794:799	barley	794:799	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	10	22	theme	direct	2266:2271	arg1	enzyme					2287:2292	an exceptional direct chain-cutting enzyme	2251:2292	an exceptional direct chain-cutting enzyme from the GH74 family	2251:2313	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	3	23	theme	enzymes	536:542	arg1	effort					516:521	a collaborative effort	500:521	a collaborative effort of different enzymes for its degradation	500:562	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	9	24	theme	β-glucans	2080:2088	arg1	polymerization					2057:2070	polymerization	2057:2070	polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains	2057:2144	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	4	25	theme	type	675:678	arg1	fermentation					680:691	a wild type fermentation	668:691	a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain)	668:748	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	6	26	theme	confocal	1277:1284	arg1	microscopy					1286:1295	confocal microscopy	1277:1295	confocal microscopy	1277:1295	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	5	27	theme	liquid	968:973	arg1	chromatography					975:988	liquid chromatography	968:988	liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases	968:1084	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	8	28	theme	glucuronoarabinoxylans	1868:1889	arg1	hydrolyzation					1834:1846	effective hydrolyzation	1824:1846	effective hydrolyzation of heavily branched glucuronoarabinoxylans	1824:1889	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	6	29	theme	component	1153:1161	arg1	analysis					1163:1170	non-starch polysaccharide component analysis	1127:1170	non-starch polysaccharide component analysis	1127:1170	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	9	30	theme	cellulose	2094:2102	arg1	polysaccharides					2104:2118	cellulose polysaccharides	2094:2118	cellulose polysaccharides	2094:2118	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	4	31	theme	multicomponent	627:640	arg1	product					655:661	a commercial multicomponent carbohydrase product	614:661	a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain)	614:748	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	10	32	theme	enzyme	2287:2292	arg1	amounts					2240:2246	significant amounts	2228:2246	significant amounts	2228:2246	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	10	32	theme	enzyme	2287:2292	arg1	enzyme					2287:2292	an exceptional direct chain-cutting enzyme	2251:2292	an exceptional direct chain-cutting enzyme from the GH74 family	2251:2313	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	4	33	theme	product	655:661	arg1	potential					601:609	the potential	597:609	the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain)	597:748	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	6	34	theme	present	1397:1403	arg1	polysaccharides					1381:1395	cellulolytic and hemicellulolytic polysaccharides	1347:1395	cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated	1347:1459	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	1	35	theme	diverse	144:150	arg1	range					152:156	A diverse range	142:156	A diverse range of monocot and dicot grains	142:184	A diverse range of monocot and dicot grains and their by-products are commonly used in the animal feed industry.					
34086701	11	36	theme	cell	2557:2560	arg1	components					2567:2576	various cell wall components	2549:2576	various cell wall components	2549:2576	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	9	37	theme	cereals	2131:2137	arg1	grains					2139:2144	viscous cereals grains	2123:2144	viscous cereals grains	2123:2144	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	11	38	dep	T.	2505:2506	arg1	reesei					2508:2513	reesei	2508:2513	reesei	2508:2513	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	6	39	theme	hemicellulolytic	1364:1379	arg1	polysaccharides					1381:1395	cellulolytic and hemicellulolytic polysaccharides	1347:1395	cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated	1347:1459	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	5	40	theme	thirty-one	871:880	arg1	proteins					899:906	thirty-one different enzyme proteins	871:906	thirty-one different enzyme proteins	871:906	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	11	41	theme	plant	2604:2608	arg1	material					2614:2621	both monocot and dicot plant raw material	2581:2621	both monocot and dicot plant raw material used as animal feed	2581:2641	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	5	42	with	chromatography	975:988	arg1	LC-MS/MS					1020:1027	tandem mass spectrometry LC-MS/MS	995:1027	tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases	995:1084	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	5	42	with	chromatography	975:988	arg1	hydrolases					1048:1057	glycosyl hydrolases	1039:1057	glycosyl hydrolases	1039:1057	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	5	42	with	chromatography	975:988	arg1	esterases					1076:1084	carbohydrate esterases	1063:1084	carbohydrate esterases	1063:1084	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	0	43	theme	walls	100:104	arg1	complexity					81:90	complexity	81:90	complexity of cell walls of plant substrates	81:124	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	5	44	theme	glycosyl	1039:1046	arg1	hydrolases					1048:1057	glycosyl hydrolases	1039:1057	glycosyl hydrolases	1039:1057	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	5	45	theme	enzyme	892:897	arg1	proteins					899:906	thirty-one different enzyme proteins	871:906	thirty-one different enzyme proteins	871:906	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	9	46	theme	different	1896:1904	arg1	β-glucanases					1906:1917	The different β-glucanases	1892:1917	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase)	1892:1973	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	11	47	theme	T.	2505:2506	arg1	secretome					2515:2523	the T. reesei secretome	2501:2523	the T. reesei secretome	2501:2523	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	3	48	theme	cell	385:388	arg1	matrix					422:427	a highly interconnected matrix	398:427	a highly interconnected matrix of various polysaccharides, proteins and lignin	398:475	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	3	48	theme	cell	385:388	arg1	wall					390:393	The cell wall	381:393	The cell wall	381:393	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	0	49	theme	Multicomponent	0:13	arg1	system					28:33	Multicomponent carbohydrase system	0:33	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.	0:140	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	9	50	from	β-glucosidase	1993:2005	arg1	fermentation					2024:2035	the T. reesei fermentation	2010:2035	the T. reesei fermentation	2010:2035	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	10	51	theme	xyloglucan-specific	2324:2342	arg1	Cel74A					2316:2321	Cel74A	2316:2321	Cel74A	2316:2321	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	10	51	theme	xyloglucan-specific	2324:2342	arg1	β-1,4-endoglucanase					2344:2362	xyloglucan-specific β-1,4-endoglucanase	2324:2362	xyloglucan-specific β-1,4-endoglucanase	2324:2362	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	4	52	theme	reesei	708:713	arg1	fermentation					680:691	a wild type fermentation	668:691	a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain)	668:748	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	6	53	theme	feed	1434:1437	arg1	ingredients					1439:1449	all the feed ingredients	1426:1449	all the feed ingredients evaluated	1426:1459	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	7	54	theme	β-glucan	1561:1568	arg1	viscosity					1504:1512	viscosity	1504:1512	viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates	1504:1579	The T. reesei fermentation also decreased viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates.					
34086701	0	55	dep	toolbox	62:68	arg1	address					73:79	address	73:79	to address complexity of cell walls of plant substrates in animal feed	70:139	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	0	56	theme	Trichoderma	40:50	arg1	reesei					52:57	Trichoderma reesei	40:57	Trichoderma reesei	40:57	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	7	57	theme	galactomannan	1543:1555	arg1	viscosity					1504:1512	viscosity	1504:1512	viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates	1504:1579	The T. reesei fermentation also decreased viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates.					
34086701	8	58	theme	debranching	1605:1615	arg1	α-galactosidase					1668:1682	α-galactosidase	1668:1682	α-galactosidase	1668:1682	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	58	theme	debranching	1605:1615	arg1	enzymes					1617:1623	several debranching enzymes	1597:1623	several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase	1597:1748	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	58	theme	debranching	1605:1615	arg1	methylesterase					1735:1748	4-O-methyl-glucuronoyl methylesterase	1712:1748	4-O-methyl-glucuronoyl methylesterase	1712:1748	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	58	theme	debranching	1605:1615	arg1	arabinofuranosidase					1635:1653	arabinofuranosidase	1635:1653	arabinofuranosidase	1635:1653	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	58	theme	debranching	1605:1615	arg1	esterase					1698:1705	acetyl xylan esterase	1685:1705	acetyl xylan esterase	1685:1705	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	58	theme	debranching	1605:1615	arg1	xylosidase					1656:1665	xylosidase	1656:1665	xylosidase	1656:1665	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	4	59	theme	wall	768:771	arg1	components					773:782	cell wall components	763:782	cell wall components of wheat	763:791	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	4	59	theme	wall	768:771	arg1	barley					794:799	barley	794:799	barley	794:799	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	11	60	from	present	2490:2496	arg1	secretome					2515:2523	the T. reesei secretome	2501:2523	the T. reesei secretome	2501:2523	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	7	61	theme	xyloglucan	1531:1540	arg1	viscosity					1504:1512	viscosity	1504:1512	viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates	1504:1579	The T. reesei fermentation also decreased viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates.					
34086701	5	62	theme	carbohydrase	941:952	arg1	product					954:960	the T. Reesei carbohydrase product	927:960	the T. Reesei carbohydrase product	927:960	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	2	63	theme	cell	295:298	arg1	structures					305:314	complex and variable cell wall structures	274:314	complex and variable cell wall structures which in turn contribute significant fiber to the complete feed	274:378	They all come with complex and variable cell wall structures which in turn contribute significant fiber to the complete feed.					
34086701	7	64	theme	arabinoxylan	1517:1528	arg1	viscosity					1504:1512	viscosity	1504:1512	viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates	1504:1579	The T. reesei fermentation also decreased viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates.					
34086701	0	65	dep	system	28:33	arg1	toolbox					62:68	A toolbox	60:68	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.	0:140	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	2	66	theme	complete	366:373	arg1	feed					375:378	the complete feed	362:378	the complete feed	362:378	They all come with complex and variable cell wall structures which in turn contribute significant fiber to the complete feed.					
34086701	8	67	theme	4-O-methyl-glucuronoyl	1712:1733	arg1	methylesterase					1735:1748	4-O-methyl-glucuronoyl methylesterase	1712:1748	4-O-methyl-glucuronoyl methylesterase	1712:1748	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	9	68	from	β-glucanases	1906:1917	arg1	fermentation					2024:2035	the T. reesei fermentation	2010:2035	the T. reesei fermentation	2010:2035	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	10	69	theme	GH74	2303:2306	arg1	family					2308:2313	the GH74 family	2299:2313	the GH74 family	2299:2313	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	7	70	dep	arabinoxylan	1517:1528	arg1	substrates					1570:1579	substrates	1570:1579	substrates	1570:1579	The T. reesei fermentation also decreased viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates.					
34086701	6	71	from	present	1397:1403	arg1	walls					1417:1421	the cell walls	1408:1421	the cell walls of all the feed ingredients evaluated	1408:1459	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	3	72	theme	interconnected	407:420	arg1	matrix					422:427	a highly interconnected matrix	398:427	a highly interconnected matrix of various polysaccharides, proteins and lignin	398:475	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	3	72	theme	interconnected	407:420	arg1	wall					390:393	The cell wall	381:393	The cell wall	381:393	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	6	73	theme	immuno-labeled	1249:1262	arg1	samples					1264:1270	immuno-labeled samples	1249:1270	immuno-labeled samples	1249:1270	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	8	74	from	xylanases	1774:1782	arg1	product					1804:1810	the carbohydrase product	1787:1810	the carbohydrase product	1787:1810	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	75	theme	GH10	1760:1763	arg1	xylanases					1774:1782	both GH10 and GH11 xylanases	1755:1782	xylanases	1774:1782	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	1	76	theme	animal	233:238	arg1	industry					245:252	the animal feed industry	229:252	the animal feed industry	229:252	A diverse range of monocot and dicot grains and their by-products are commonly used in the animal feed industry.					
34086701	10	77	theme	reesei	2211:2216	arg1	secretome					2195:2203	the secretome	2191:2203	the secretome of T. reesei	2191:2216	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	8	78	theme	xylan	1692:1696	arg1	esterase					1698:1705	acetyl xylan esterase	1685:1705	acetyl xylan esterase	1685:1705	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	79	theme	GH11	1769:1772	arg1	xylanases					1774:1782	both GH10 and GH11 xylanases	1755:1782	xylanases	1774:1782	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	9	80	from	cellulases	1976:1985	arg1	fermentation					2024:2035	the T. reesei fermentation	2010:2035	the T. reesei fermentation	2010:2035	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	6	81	theme	confocal	1218:1225	arg1	microscopy					1227:1236	confocal microscopy	1218:1236	confocal microscopy	1218:1236	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	1	82	theme	monocot	161:167	arg1	grains					179:184	monocot and dicot grains	161:184	monocot and dicot grains	161:184	A diverse range of monocot and dicot grains and their by-products are commonly used in the animal feed industry.					
34086701	4	83	theme	wheat	787:791	arg1	components					773:782	cell wall components	763:782	cell wall components of wheat	763:791	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	4	83	theme	wheat	787:791	arg1	cassava					851:857	cassava	851:857	cassava	851:857	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	4	83	theme	wheat	787:791	arg1	barley					794:799	barley	794:799	barley	794:799	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	1	84	used	used	221:224	arg2	range					152:156	A diverse range	142:156	A diverse range of monocot and dicot grains	142:184	A diverse range of monocot and dicot grains and their by-products are commonly used in the animal feed industry.					
34086701	1	84	used	used	221:224	arg2	by-products					196:206	their by-products	190:206	their by-products	190:206	A diverse range of monocot and dicot grains and their by-products are commonly used in the animal feed industry.					
34086701	0	85	theme	substrates	115:124	arg1	walls					100:104	cell walls	95:104	cell walls of plant substrates	95:124	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	8	86	theme	carbohydrase	1791:1802	arg1	product					1804:1810	the carbohydrase product	1787:1810	the carbohydrase product	1787:1810	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	1	87	theme	dicot	173:177	arg1	grains					179:184	monocot and dicot grains	161:184	monocot and dicot grains	161:184	A diverse range of monocot and dicot grains and their by-products are commonly used in the animal feed industry.					
34086701	10	88	theme	significant	2228:2238	arg1	amounts					2240:2246	significant amounts	2228:2246	significant amounts	2228:2246	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	10	88	theme	significant	2228:2238	arg1	enzyme					2287:2292	an exceptional direct chain-cutting enzyme	2251:2292	an exceptional direct chain-cutting enzyme from the GH74 family	2251:2313	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	2	89	theme	wall	300:303	arg1	structures					305:314	complex and variable cell wall structures	274:314	complex and variable cell wall structures which in turn contribute significant fiber to the complete feed	274:378	They all come with complex and variable cell wall structures which in turn contribute significant fiber to the complete feed.					
34086701	0	90	theme	animal	129:134	arg1	feed					136:139	animal feed	129:139	animal feed	129:139	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	5	91	theme	tandem	995:1000	arg1	LC-MS/MS					1020:1027	tandem mass spectrometry LC-MS/MS	995:1027	tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases	995:1084	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	5	91	theme	tandem	995:1000	arg1	hydrolases					1048:1057	glycosyl hydrolases	1039:1057	glycosyl hydrolases	1039:1057	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	5	91	theme	tandem	995:1000	arg1	esterases					1076:1084	carbohydrate esterases	1063:1084	carbohydrate esterases	1063:1084	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	10	92	theme	substituted	2419:2429	arg1	regions					2431:2437	the substituted regions	2415:2437	the substituted regions	2415:2437	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	11	93	theme	raw	2610:2612	arg1	material					2614:2621	both monocot and dicot plant raw material	2581:2621	both monocot and dicot plant raw material used as animal feed	2581:2641	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	10	94	theme	chain-cutting	2273:2285	arg1	enzyme					2287:2292	an exceptional direct chain-cutting enzyme	2251:2292	an exceptional direct chain-cutting enzyme from the GH74 family	2251:2313	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	5	95	theme	spectrometry	1007:1018	arg1	LC-MS/MS					1020:1027	tandem mass spectrometry LC-MS/MS	995:1027	tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases	995:1084	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	5	95	theme	spectrometry	1007:1018	arg1	hydrolases					1048:1057	glycosyl hydrolases	1039:1057	glycosyl hydrolases	1039:1057	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	5	95	theme	spectrometry	1007:1018	arg1	esterases					1076:1084	carbohydrate esterases	1063:1084	carbohydrate esterases	1063:1084	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	3	96	theme	different	526:534	arg1	enzymes					536:542	different enzymes	526:542	different enzymes	526:542	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	8	97	theme	effective	1824:1832	arg1	hydrolyzation					1834:1846	effective hydrolyzation	1824:1846	effective hydrolyzation of heavily branched glucuronoarabinoxylans	1824:1889	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	4	98	dep	reesei	708:713	arg1	reesei					719:724	T. reesei	716:724	T. reesei	716:724	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	4	99	theme	de-oiled	807:814	arg1	bran					821:824	de-oiled rice bran	807:824	de-oiled rice bran	807:824	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	4	99	theme	de-oiled	807:814	arg1	barley					794:799	barley	794:799	barley	794:799	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	10	100	theme	exceptional	2254:2264	arg1	enzyme					2287:2292	an exceptional direct chain-cutting enzyme	2251:2292	an exceptional direct chain-cutting enzyme from the GH74 family	2251:2313	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	10	101	from	family	2308:2313	arg1	amounts					2240:2246	significant amounts	2228:2246	significant amounts	2228:2246	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	10	101	from	family	2308:2313	arg1	enzyme					2287:2292	an exceptional direct chain-cutting enzyme	2251:2292	an exceptional direct chain-cutting enzyme from the GH74 family	2251:2313	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	6	102	from	walls	1417:1421	arg1	present					1397:1403	present	1397:1403	present	1397:1403	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	11	103	theme	enzymes	2482:2488	arg1	capable					2528:2534	capable	2528:2534	capable	2528:2534	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	11	103	theme	enzymes	2482:2488	arg1	balance					2471:2477	the balance	2467:2477	the balance of enzymes present in the T. reesei secretome	2467:2523	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	11	104	theme	animal	2631:2636	arg1	feed					2638:2641	animal feed	2631:2641	animal feed	2631:2641	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	9	105	dep	T.	2014:2015	arg1	reesei					2017:2022	reesei	2017:2022	reesei	2017:2022	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	4	106	theme	wild	670:673	arg1	fermentation					680:691	a wild type fermentation	668:691	a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain)	668:748	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	3	107	theme	various	432:438	arg1	polysaccharides					440:454	various polysaccharides	432:454	various polysaccharides	432:454	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	6	108	theme	cell	1412:1415	arg1	walls					1417:1421	the cell walls	1408:1421	the cell walls of all the feed ingredients evaluated	1408:1459	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	8	109	theme	branched	1859:1866	arg1	glucuronoarabinoxylans					1868:1889	heavily branched glucuronoarabinoxylans	1851:1889	heavily branched glucuronoarabinoxylans	1851:1889	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	6	110	theme	polysaccharide	1138:1151	arg1	analysis					1163:1170	non-starch polysaccharide component analysis	1127:1170	non-starch polysaccharide component analysis	1127:1170	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	9	111	theme	polysaccharides	2104:2118	arg1	polymerization					2057:2070	polymerization	2057:2070	polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains	2057:2144	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	4	112	theme	commercial	616:625	arg1	product					655:661	a commercial multicomponent carbohydrase product	614:661	a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain)	614:748	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	5	113	theme	carbohydrate	1063:1074	arg1	esterases					1076:1084	carbohydrate esterases	1063:1084	carbohydrate esterases	1063:1084	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	11	114	theme	wall	2562:2565	arg1	components					2567:2576	various cell wall components	2549:2576	various cell wall components	2549:2576	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	9	115	theme	viscous	2123:2129	arg1	grains					2139:2144	viscous cereals grains	2123:2144	viscous cereals grains	2123:2144	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	4	116	theme	carbohydrase	642:653	arg1	product					655:661	a commercial multicomponent carbohydrase product	614:661	a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain)	614:748	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	0	117	theme	cell	95:98	arg1	walls					100:104	cell walls	95:104	cell walls of plant substrates	95:124	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	11	118	theme	dicot	2598:2602	arg1	material					2614:2621	both monocot and dicot plant raw material	2581:2621	both monocot and dicot plant raw material used as animal feed	2581:2641	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	9	119	theme	grains	2139:2144	arg1	polymerization					2057:2070	polymerization	2057:2070	polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains	2057:2144	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	3	120	theme	polysaccharides	440:454	arg1	matrix					422:427	a highly interconnected matrix	398:427	a highly interconnected matrix of various polysaccharides, proteins and lignin	398:475	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	3	120	theme	polysaccharides	440:454	arg1	wall					390:393	The cell wall	381:393	The cell wall	381:393	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	5	121	theme	different	882:890	arg1	proteins					899:906	thirty-one different enzyme proteins	871:906	thirty-one different enzyme proteins	871:906	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	0	122	from	reesei	52:57	arg1	system					28:33	Multicomponent carbohydrase system	0:33	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.	0:140	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	0	123	theme	carbohydrase	15:26	arg1	system					28:33	Multicomponent carbohydrase system	0:33	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.	0:140	Multicomponent carbohydrase system from Trichoderma reesei: A toolbox to address complexity of cell walls of plant substrates in animal feed.					
34086701	6	124	attach	present	1397:1403	arg1	walls					1417:1421	the cell walls	1408:1421	the cell walls of all the feed ingredients evaluated	1408:1459	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	6	124	attach	present	1397:1403	arg2	polysaccharides					1381:1395	cellulolytic and hemicellulolytic polysaccharides	1347:1395	cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated	1347:1459	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	2	125	theme	significant	341:351	arg1	fiber					353:357	significant fiber	341:357	significant fiber	341:357	They all come with complex and variable cell wall structures which in turn contribute significant fiber to the complete feed.					
34086701	6	126	theme	ingredients	1439:1449	arg1	walls					1417:1421	the cell walls	1408:1421	the cell walls of all the feed ingredients evaluated	1408:1459	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	4	127	from	fermentation	680:691	arg1	potential					601:609	the potential	597:609	the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain)	597:748	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	4	127	from	fermentation	680:691	arg1	product					655:661	a commercial multicomponent carbohydrase product	614:661	a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain)	614:748	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	8	128	theme	several	1597:1603	arg1	α-galactosidase					1668:1682	α-galactosidase	1668:1682	α-galactosidase	1668:1682	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	128	theme	several	1597:1603	arg1	enzymes					1617:1623	several debranching enzymes	1597:1623	several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase	1597:1748	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	128	theme	several	1597:1603	arg1	methylesterase					1735:1748	4-O-methyl-glucuronoyl methylesterase	1712:1748	4-O-methyl-glucuronoyl methylesterase	1712:1748	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	128	theme	several	1597:1603	arg1	arabinofuranosidase					1635:1653	arabinofuranosidase	1635:1653	arabinofuranosidase	1635:1653	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	128	theme	several	1597:1603	arg1	esterase					1698:1705	acetyl xylan esterase	1685:1705	acetyl xylan esterase	1685:1705	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	8	128	theme	several	1597:1603	arg1	xylosidase					1656:1665	xylosidase	1656:1665	xylosidase	1656:1665	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	11	129	from	secretome	2515:2523	arg1	present					2490:2496	present	2490:2496	present	2490:2496	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	8	130	theme	enzymes	1617:1623	arg1	Combination					1582:1592	Combination	1582:1592	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product	1582:1810	Combination of several debranching enzymes including arabinofuranosidase, xylosidase, α-galactosidase, acetyl xylan esterase, and 4-O-methyl-glucuronoyl methylesterase with both GH10 and GH11 xylanases in the carbohydrase product resulted in effective hydrolyzation of heavily branched glucuronoarabinoxylans.					
34086701	6	131	theme	cellulolytic	1347:1358	arg1	polysaccharides					1381:1395	cellulolytic and hemicellulolytic polysaccharides	1347:1395	cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated	1347:1459	As measured by in vitro incubations and non-starch polysaccharide component analysis, and visualization by immunocytochemistry and confocal microscopy imaging of immuno-labeled samples with confocal microscopy, the carbohydrase product effectively solubilized cellulolytic and hemicellulolytic polysaccharides present in the cell walls of all the feed ingredients evaluated.					
34086701	7	132	dep	T.	1466:1467	arg1	reesei					1469:1474	reesei	1469:1474	reesei	1469:1474	The T. reesei fermentation also decreased viscosity of arabinoxylan, xyloglucan, galactomannan and β-glucan substrates.					
34086701	9	133	dep	reduced	2049:2055	arg1	wheat					2147:2151	wheat	2147:2151	wheat	2147:2151	The different β-glucanases (both endo-β-1,3(4)-glucanase and endo-β-1,3-glucanase), cellulases and a β-glucosidase in the T. reesei fermentation effectively reduced polymerization of both β-glucans and cellulose polysaccharides of viscous cereals grains (wheat, barley, rye and oat).					
34086701	5	134	theme	Reesei	934:939	arg1	product					954:960	the T. Reesei carbohydrase product	927:960	the T. Reesei carbohydrase product	927:960	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	11	135	theme	monocot	2586:2592	arg1	material					2614:2621	both monocot and dicot plant raw material	2581:2621	both monocot and dicot plant raw material used as animal feed	2581:2641	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	4	136	theme	cell	763:766	arg1	components					773:782	cell wall components	763:782	cell wall components of wheat	763:791	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	4	136	theme	cell	763:766	arg1	barley					794:799	barley	794:799	barley	794:799	In this regard, we investigated the potential of a commercial multicomponent carbohydrase product from a wild type fermentation of Trichoderma reesei (T. reesei) (RONOZYME® MultiGrain) in degrading cell wall components of wheat, barley, rye, de-oiled rice bran, sunflower, rapeseed and cassava.					
34086701	3	137	theme	proteins	457:464	arg1	matrix					422:427	a highly interconnected matrix	398:427	a highly interconnected matrix of various polysaccharides, proteins and lignin	398:475	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	3	137	theme	proteins	457:464	arg1	wall					390:393	The cell wall	381:393	The cell wall	381:393	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	5	138	theme	proteins	899:906	arg1	total					862:866	A total	860:866	A total of thirty-one different enzyme proteins	860:906	A total of thirty-one different enzyme proteins were identified in the T. Reesei carbohydrase product using liquid chromatography with tandem mass spectrometry LC-MS/MS including glycosyl hydrolases and carbohydrate esterases.					
34086701	10	139	dep	amounts	2240:2246	arg1	Cel74A					2316:2321	Cel74A	2316:2321	Cel74A	2316:2321	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	10	139	dep	amounts	2240:2246	arg1	β-1,4-endoglucanase					2344:2362	xyloglucan-specific β-1,4-endoglucanase	2324:2362	xyloglucan-specific β-1,4-endoglucanase	2324:2362	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	10	140	theme	xyloglucan	2392:2401	arg1	backbone					2403:2410	the xyloglucan backbone	2388:2410	the xyloglucan backbone at the substituted regions	2388:2437	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	10	141	from	regions	2431:2437	arg1	backbone					2403:2410	the xyloglucan backbone	2388:2410	the xyloglucan backbone at the substituted regions	2388:2437	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
34086701	2	142	theme	variable	286:293	arg1	structures					305:314	complex and variable cell wall structures	274:314	complex and variable cell wall structures which in turn contribute significant fiber to the complete feed	274:378	They all come with complex and variable cell wall structures which in turn contribute significant fiber to the complete feed.					
34086701	3	143	dep	matrix	422:427	arg1	requires					491:498	requires	491:498	requires a collaborative effort of different enzymes for its degradation	491:562	The cell wall is a highly interconnected matrix of various polysaccharides, proteins and lignin and, as such, requires a collaborative effort of different enzymes for its degradation.					
34086701	11	144	theme	various	2549:2555	arg1	components					2567:2576	various cell wall components	2549:2576	various cell wall components	2549:2576	Here, we demonstrated that the balance of enzymes present in the T. reesei secretome is capable of degrading various cell wall components in both monocot and dicot plant raw material used as animal feed.					
34086701	10	145	from	amounts	2240:2246	arg1	family					2308:2313	the GH74 family	2299:2313	the GH74 family	2299:2313	Interestingly, the secretome of T. reesei contained significant amounts of an exceptional direct chain-cutting enzyme from the GH74 family (Cel74A, xyloglucan-specific β-1,4-endoglucanase), that strictly cleaves the xyloglucan backbone at the substituted regions.					
33851227	7	0	from	activities	1408:1417	arg1	account					1350:1356	the account	1346:1356	the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD	1346:1565	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	0	from	activities	1408:1417	arg1	spectrum					1451:1458	the mass spectrum	1442:1458	the mass spectrum	1442:1458	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	8	1	from	effects	1631:1637	arg1	SJZD					1671:1674	SJZD	1671:1674	SJZD	1671:1674	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	7	2	theme	quantitative	1538:1549	arg1	markers					1551:1557	qualitative or quantitative markers	1523:1557	qualitative or quantitative markers of SJZD	1523:1565	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	6	3	theme	mass	1265:1268	arg1	UHPLC-QTRAP®-MS/MS					1284:1301	UHPLC-QTRAP®-MS/MS	1284:1301	UHPLC-QTRAP®-MS/MS	1284:1301	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	6	3	theme	mass	1265:1268	arg1	spectrometry					1270:1281	triple-quadrupole linear ion-trap tandem mass spectrometry	1224:1281	triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS)	1224:1302	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	8	4	theme	analytical	1699:1708	arg1	approach					1710:1717	a useful analytical approach	1690:1717	a useful analytical approach for complex composition research of TCMs	1690:1758	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	3	5	theme	offline	551:557	arg1	chromatography					582:595	offline two-dimensional liquid chromatography	551:595	offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS)	551:677	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	7	6	theme	qualitative	1523:1533	arg1	markers					1551:1557	qualitative or quantitative markers	1523:1557	qualitative or quantitative markers of SJZD	1523:1565	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	6	7	theme	ion-trap	1249:1256	arg1	UHPLC-QTRAP®-MS/MS					1284:1301	UHPLC-QTRAP®-MS/MS	1284:1301	UHPLC-QTRAP®-MS/MS	1284:1301	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	6	7	theme	ion-trap	1249:1256	arg1	spectrometry					1270:1281	triple-quadrupole linear ion-trap tandem mass spectrometry	1224:1281	triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS)	1224:1302	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	6	8	theme	triple-quadrupole	1224:1240	arg1	UHPLC-QTRAP®-MS/MS					1284:1301	UHPLC-QTRAP®-MS/MS	1284:1301	UHPLC-QTRAP®-MS/MS	1284:1301	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	6	8	theme	triple-quadrupole	1224:1240	arg1	spectrometry					1270:1281	triple-quadrupole linear ion-trap tandem mass spectrometry	1224:1281	triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS)	1224:1302	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	5	9	theme	forty-nine	909:918	arg1	components					920:929	Four hundred forty-nine components	896:929	Four hundred forty-nine components	896:929	Four hundred forty-nine components, among which 6 were potentially novel, and 32 were confirmed by standard substances, were identified or tentatively assigned.					
33851227	3	10	theme	liquid	575:580	arg1	chromatography					582:595	offline two-dimensional liquid chromatography	551:595	offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS)	551:677	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	1	11	theme	classic	220:226	arg1	recipe					228:233	a classic recipe	218:233	a classic recipe in traditional Chinese medicine (TCM)	218:271	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	1	11	theme	classic	220:226	arg1	decoction					200:208	Sijunzi decoction	192:208	Sijunzi decoction (SJZD)	192:215	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	7	12	theme	mass	1446:1449	arg1	spectrum					1451:1458	the mass spectrum	1442:1458	the mass spectrum	1442:1458	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	13	theme	in	1399:1400	arg1	activities					1408:1417	in vivo or in vitro activities	1388:1417	in vivo or in vitro activities	1388:1417	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	3	14	theme	tandem	636:641	arg1	spectrometry					648:659	quadrupole time-of-flight tandem mass spectrometry	610:659	quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS)	610:677	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	3	14	theme	tandem	636:641	arg1	2DLC-QTOF-MS/MS					662:676	2DLC-QTOF-MS/MS	662:676	2DLC-QTOF-MS/MS	662:676	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	1	15	from	recipe	228:233	arg1	medicine					258:265	traditional Chinese medicine	238:265	traditional Chinese medicine (TCM)	238:271	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	1	15	from	recipe	228:233	arg1	TCM					268:270	TCM	268:270	TCM	268:270	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	3	16	theme	quadrupole	610:619	arg1	spectrometry					648:659	quadrupole time-of-flight tandem mass spectrometry	610:659	quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS)	610:677	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	3	16	theme	quadrupole	610:619	arg1	2DLC-QTOF-MS/MS					662:676	2DLC-QTOF-MS/MS	662:676	2DLC-QTOF-MS/MS	662:676	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	0	17	from	quantification	128:141	arg1	decoction					181:189	Sijunzi decoction	173:189	Sijunzi decoction	173:189	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	7	18	theme	peak	1424:1427	arg1	intensity					1429:1437	the peak intensity	1420:1437	the peak intensity in the mass spectrum	1420:1458	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	0	19	from	characterization	107:122	arg1	decoction					181:189	Sijunzi decoction	173:189	Sijunzi decoction	173:189	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	7	20	theme	in	1388:1389	arg1	activities					1408:1417	in vivo or in vitro activities	1388:1417	in vivo or in vitro activities	1388:1417	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	1	21	theme	diseases	338:345	arg1	treatment					308:316	the clinical treatment	295:316	the clinical treatment of gastrointestinal diseases	295:345	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	8	22	theme	useful	1692:1697	arg1	approach					1710:1717	a useful analytical approach	1690:1717	a useful analytical approach for complex composition research of TCMs	1690:1758	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	0	23	dep	characterization	107:122	arg1	the					103:105	the	103:105	the	103:105	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	2	24	theme	non-polysaccharides	461:479	arg1	composition					446:456	the composition	442:456	the composition of non-polysaccharides (NPSs)	442:486	While there are reports on pharmaceutical substances of SJZD focusing on its polysaccharides, the composition of non-polysaccharides (NPSs) has not yet been holistically clarified.					
33851227	3	25	used	used	683:686	arg2	chromatography					582:595	offline two-dimensional liquid chromatography	551:595	offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS)	551:677	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	4	26	theme	SJZD	769:772	arg1	MS-network					755:764	the MS-network	751:764	the MS-network of SJZD	751:772	In addition, the MS-network of SJZD was proposed, which led to the construction of a larger in-house chemical library and accelerated qualitative processing.					
33851227	6	27	theme	method	1084:1089	arg1	validation					1091:1100	good method validation	1079:1100	good method validation	1079:1100	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	8	28	theme	effects	1631:1637	arg1	understanding					1594:1606	understanding	1594:1606	understanding of the pharmacological effects and action mechanism of NPSs in SJZD	1594:1674	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	4	29	theme	in-house	830:837	arg1	library					848:854	a larger in-house chemical library	821:854	a larger in-house chemical library	821:854	In addition, the MS-network of SJZD was proposed, which led to the construction of a larger in-house chemical library and accelerated qualitative processing.					
33851227	0	30	theme	MS-network-based	26:41	arg1	offline					43:49	MS-network-based offline	26:49	MS-network-based offline	26:49	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	1	31	theme	clinical	299:306	arg1	treatment					308:316	the clinical treatment	295:316	the clinical treatment of gastrointestinal diseases	295:345	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	7	32	dep	in	1399:1400	arg1	vitro					1402:1406	vitro	1402:1406	vitro	1402:1406	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	33	from	reports	1377:1383	arg1	intensity					1429:1437	the peak intensity	1420:1437	the peak intensity in the mass spectrum	1420:1458	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	33	from	reports	1377:1383	arg1	structures					1480:1489	characteristic structures	1465:1489	characteristic structures	1465:1489	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	33	from	reports	1377:1383	arg1	activities					1408:1417	in vivo or in vitro activities	1388:1417	in vivo or in vitro activities	1388:1417	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	8	34	theme	action	1643:1648	arg1	mechanism					1650:1658	action mechanism	1643:1658	action mechanism	1643:1658	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	4	35	theme	library	848:854	arg1	construction					805:816	the construction	801:816	the construction of a larger in-house chemical library	801:854	In addition, the MS-network of SJZD was proposed, which led to the construction of a larger in-house chemical library and accelerated qualitative processing.					
33851227	6	36	theme	ultra-high-performance	1166:1187	arg1	chromatography					1196:1209	ultra-high-performance liquid chromatography	1166:1209	ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS)	1166:1302	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	2	37	from	reports	364:370	arg1	substances					390:399	pharmaceutical substances	375:399	pharmaceutical substances of SJZD focusing on its polysaccharides	375:439	While there are reports on pharmaceutical substances of SJZD focusing on its polysaccharides, the composition of non-polysaccharides (NPSs) has not yet been holistically clarified.					
33851227	0	38	dep	2DLC-QTOF-MS/MS	51:65	arg1	coupled					67:73	coupled	67:73	2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction	51:189	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	1	39	theme	traditional	238:248	arg1	medicine					258:265	traditional Chinese medicine	238:265	traditional Chinese medicine (TCM)	238:271	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	1	39	theme	traditional	238:248	arg1	TCM					268:270	TCM	268:270	TCM	268:270	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	7	40	theme	SJZD	1562:1565	arg1	markers					1551:1557	qualitative or quantitative markers	1523:1557	qualitative or quantitative markers of SJZD	1523:1565	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	3	41	theme	NPS	706:708	arg1	profiling					719:727	comprehensive NPS chemical profiling	692:727	comprehensive NPS chemical profiling of SJZD	692:735	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	2	42	theme	pharmaceutical	375:388	arg1	substances					390:399	pharmaceutical substances	375:399	pharmaceutical substances of SJZD focusing on its polysaccharides	375:439	While there are reports on pharmaceutical substances of SJZD focusing on its polysaccharides, the composition of non-polysaccharides (NPSs) has not yet been holistically clarified.					
33851227	8	43	theme	complex	1723:1729	arg1	research					1743:1750	complex composition research	1723:1750	complex composition research of TCMs	1723:1758	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	6	44	theme	representative	1106:1119	arg1	components					1121:1130	19 representative components	1103:1130	19 representative components	1103:1130	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	3	45	theme	SJZD	732:735	arg1	profiling					719:727	comprehensive NPS chemical profiling	692:727	comprehensive NPS chemical profiling of SJZD	692:735	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	8	46	theme	TCMs	1755:1758	arg1	research					1743:1750	complex composition research	1723:1750	complex composition research of TCMs	1723:1758	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	7	47	theme	characteristic	1465:1478	arg1	structures					1480:1489	characteristic structures	1465:1489	characteristic structures	1465:1489	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	6	48	theme	tandem	1258:1263	arg1	UHPLC-QTRAP®-MS/MS					1284:1301	UHPLC-QTRAP®-MS/MS	1284:1301	UHPLC-QTRAP®-MS/MS	1284:1301	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	6	48	theme	tandem	1258:1263	arg1	spectrometry					1270:1281	triple-quadrupole linear ion-trap tandem mass spectrometry	1224:1281	triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS)	1224:1302	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	0	49	from	decoction	181:189	arg1	quantification					128:141	quantification	128:141	quantification	128:141	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	0	49	from	decoction	181:189	arg1	characterization					107:122	characterization	107:122	characterization	107:122	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	8	50	from	NPSs	1663:1666	arg1	SJZD					1671:1674	SJZD	1671:1674	SJZD	1671:1674	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	3	51	theme	current	536:542	arg1	study					544:548	the current study	532:548	the current study	532:548	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	7	52	from	account	1350:1356	arg1	intensity					1429:1437	the peak intensity	1420:1437	the peak intensity in the mass spectrum	1420:1458	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	52	from	account	1350:1356	arg1	structures					1480:1489	characteristic structures	1465:1489	characteristic structures	1465:1489	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	52	from	account	1350:1356	arg1	activities					1408:1417	in vivo or in vitro activities	1388:1417	in vivo or in vitro activities	1388:1417	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	6	53	theme	linear	1242:1247	arg1	UHPLC-QTRAP®-MS/MS					1284:1301	UHPLC-QTRAP®-MS/MS	1284:1301	UHPLC-QTRAP®-MS/MS	1284:1301	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	6	53	theme	linear	1242:1247	arg1	spectrometry					1270:1281	triple-quadrupole linear ion-trap tandem mass spectrometry	1224:1281	triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS)	1224:1302	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	8	54	from	mechanism	1650:1658	arg1	SJZD					1671:1674	SJZD	1671:1674	SJZD	1671:1674	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	4	55	theme	qualitative	872:882	arg1	processing					884:893	qualitative processing	872:893	qualitative processing	872:893	In addition, the MS-network of SJZD was proposed, which led to the construction of a larger in-house chemical library and accelerated qualitative processing.					
33851227	0	56	theme	non-polysaccharides	150:168	arg1	quantification					128:141	quantification	128:141	quantification	128:141	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	0	56	theme	non-polysaccharides	150:168	arg1	characterization					107:122	characterization	107:122	characterization	107:122	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	3	57	theme	two-dimensional	559:573	arg1	chromatography					582:595	offline two-dimensional liquid chromatography	551:595	offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS)	551:677	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	7	58	theme	bioactive	1367:1375	arg1	reports					1377:1383	their bioactive reports	1361:1383	their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD	1361:1565	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	8	59	from	SJZD	1671:1674	arg1	effects					1631:1637	pharmacological effects	1615:1637	pharmacological effects	1615:1637	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	8	59	from	SJZD	1671:1674	arg1	mechanism					1650:1658	action mechanism	1643:1658	action mechanism	1643:1658	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	3	60	theme	time-of-flight	621:634	arg1	spectrometry					648:659	quadrupole time-of-flight tandem mass spectrometry	610:659	quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS)	610:677	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	3	60	theme	time-of-flight	621:634	arg1	2DLC-QTOF-MS/MS					662:676	2DLC-QTOF-MS/MS	662:676	2DLC-QTOF-MS/MS	662:676	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	1	61	theme	gastrointestinal	321:336	arg1	diseases					338:345	gastrointestinal diseases	321:345	gastrointestinal diseases	321:345	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	8	62	dep	effects	1631:1637	arg1	the					1611:1613	the	1611:1613	the	1611:1613	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	3	63	theme	mass	643:646	arg1	spectrometry					648:659	quadrupole time-of-flight tandem mass spectrometry	610:659	quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS)	610:677	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	3	63	theme	mass	643:646	arg1	2DLC-QTOF-MS/MS					662:676	2DLC-QTOF-MS/MS	662:676	2DLC-QTOF-MS/MS	662:676	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	7	64	from	structures	1480:1489	arg1	account					1350:1356	the account	1346:1356	the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD	1346:1565	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	64	from	structures	1480:1489	arg1	spectrum					1451:1458	the mass spectrum	1442:1458	the mass spectrum	1442:1458	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	1	65	theme	Sijunzi	192:198	arg1	recipe					228:233	a classic recipe	218:233	a classic recipe in traditional Chinese medicine (TCM)	218:271	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	1	65	theme	Sijunzi	192:198	arg1	SJZD					211:214	SJZD	211:214	SJZD	211:214	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	1	65	theme	Sijunzi	192:198	arg1	decoction					200:208	Sijunzi decoction	192:208	Sijunzi decoction (SJZD)	192:215	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	8	66	theme	present	1572:1578	arg1	work					1580:1583	The present work	1568:1583	The present work	1568:1583	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	5	67	theme	standard	995:1002	arg1	substances					1004:1013	standard substances	995:1013	standard substances	995:1013	Four hundred forty-nine components, among which 6 were potentially novel, and 32 were confirmed by standard substances, were identified or tentatively assigned.					
33851227	2	68	theme	SJZD	404:407	arg1	substances					390:399	pharmaceutical substances	375:399	pharmaceutical substances of SJZD focusing on its polysaccharides	375:439	While there are reports on pharmaceutical substances of SJZD focusing on its polysaccharides, the composition of non-polysaccharides (NPSs) has not yet been holistically clarified.					
33851227	0	69	theme	integrated	3:12	arg1	strategy					14:21	An integrated strategy	0:21	An integrated strategy of MS-network-based offline	0:49	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	7	70	from	intensity	1429:1437	arg1	account					1350:1356	the account	1346:1356	the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD	1346:1565	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	70	from	intensity	1429:1437	arg1	spectrum					1451:1458	the mass spectrum	1442:1458	the mass spectrum	1442:1458	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	0	71	theme	Sijunzi	173:179	arg1	decoction					181:189	Sijunzi decoction	173:189	Sijunzi decoction	173:189	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	8	72	theme	pharmacological	1615:1629	arg1	effects					1631:1637	pharmacological effects	1615:1637	pharmacological effects	1615:1637	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	6	73	theme	good	1079:1082	arg1	validation					1091:1100	good method validation	1079:1100	good method validation	1079:1100	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	0	74	theme	offline	43:49	arg1	strategy					14:21	An integrated strategy	0:21	An integrated strategy of MS-network-based offline	0:49	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33851227	7	75	theme	reports	1377:1383	arg1	account					1350:1356	the account	1346:1356	the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD	1346:1565	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	8	76	theme	mechanism	1650:1658	arg1	understanding					1594:1606	understanding	1594:1606	understanding of the pharmacological effects and action mechanism of NPSs in SJZD	1594:1674	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	4	77	theme	larger	823:828	arg1	library					848:854	a larger in-house chemical library	821:854	a larger in-house chemical library	821:854	In addition, the MS-network of SJZD was proposed, which led to the construction of a larger in-house chemical library and accelerated qualitative processing.					
33851227	6	78	theme	liquid	1189:1194	arg1	chromatography					1196:1209	ultra-high-performance liquid chromatography	1166:1209	ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS)	1166:1302	Furthermore, based on good method validation, 19 representative components were simultaneously quantified by ultra-high-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UHPLC-QTRAP®-MS/MS).					
33851227	8	79	theme	NPSs	1663:1666	arg1	effects					1631:1637	pharmacological effects	1615:1637	pharmacological effects	1615:1637	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	8	79	theme	NPSs	1663:1666	arg1	mechanism					1650:1658	action mechanism	1643:1658	action mechanism	1643:1658	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	3	80	theme	chemical	710:717	arg1	profiling					719:727	comprehensive NPS chemical profiling	692:727	comprehensive NPS chemical profiling of SJZD	692:735	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	4	81	theme	chemical	839:846	arg1	library					848:854	a larger in-house chemical library	821:854	a larger in-house chemical library	821:854	In addition, the MS-network of SJZD was proposed, which led to the construction of a larger in-house chemical library and accelerated qualitative processing.					
33851227	7	82	contain	have	1498:1501	arg2	potential					1507:1515	the potential to be qualitative or quantitative markers of SJZD	1503:1565	the potential to be qualitative or quantitative markers of SJZD	1503:1565	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	82	contain	have	1498:1501	arg1	intensity					1429:1437	the peak intensity	1420:1437	the peak intensity in the mass spectrum	1420:1458	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	82	contain	have	1498:1501	arg1	structures					1480:1489	characteristic structures	1465:1489	characteristic structures	1465:1489	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	7	82	contain	have	1498:1501	arg1	activities					1408:1417	in vivo or in vitro activities	1388:1417	in vivo or in vitro activities	1388:1417	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	3	83	theme	comprehensive	692:704	arg1	profiling					719:727	comprehensive NPS chemical profiling	692:727	comprehensive NPS chemical profiling of SJZD	692:735	In the current study, offline two-dimensional liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (2DLC-QTOF-MS/MS) was used for comprehensive NPS chemical profiling of SJZD.					
33851227	8	84	theme	composition	1731:1741	arg1	research					1743:1750	complex composition research	1723:1750	complex composition research of TCMs	1723:1758	The present work furthers understanding of the pharmacological effects and action mechanism of NPSs in SJZD, and provides a useful analytical approach for complex composition research of TCMs.					
33851227	1	85	theme	Chinese	250:256	arg1	medicine					258:265	traditional Chinese medicine	238:265	traditional Chinese medicine (TCM)	238:271	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	1	85	theme	Chinese	250:256	arg1	TCM					268:270	TCM	268:270	TCM	268:270	Sijunzi decoction (SJZD), a classic recipe in traditional Chinese medicine (TCM), has been applied for the clinical treatment of gastrointestinal diseases.					
33851227	7	86	dep	in	1388:1389	arg1	vivo					1391:1394	vivo	1391:1394	vivo	1391:1394	They were selected for quantification on the account of their bioactive reports on in vivo or in vitro activities, the peak intensity in the mass spectrum, and characteristic structures, which have the potential to be qualitative or quantitative markers of SJZD.					
33851227	0	87	from	non-polysaccharides	150:168	arg1	decoction					181:189	Sijunzi decoction	173:189	Sijunzi decoction	173:189	An integrated strategy of MS-network-based offline 2DLC-QTOF-MS/MS coupled with UHPLC-QTRAP®-MS/MS for the characterization and quantification of the non-polysaccharides in Sijunzi decoction.					
33091474	5	0	contain	having	676:681	arg2	size					685:688	a size	683:688	a size of 11.92 nm	683:700	Further analysis with XRD confirmed the presence of crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%.					
33091474	5	0	contain	having	676:681	arg1	cellulose					666:674	crystalline cellulose	654:674	crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%	654:737	Further analysis with XRD confirmed the presence of crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%.					
33091474	5	1	theme	crystalline	654:664	arg1	cellulose					666:674	crystalline cellulose	654:674	crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%	654:737	Further analysis with XRD confirmed the presence of crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%.					
33091474	5	2	with	analysis	610:617	arg1	XRD					624:626	XRD	624:626	XRD	624:626	Further analysis with XRD confirmed the presence of crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%.					
33091474	9	3	theme	synthetic	1329:1337	arg1	fibers					1339:1344	synthetic fibers	1329:1344	synthetic fibers in polymer matrix composites	1329:1373	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	4	4	theme	cellulose	505:513	arg1	contents					542:549	the cellulose, hemicellulose, and lignin contents	501:549	the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively	501:599	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33091474	7	5	theme	mean	900:903	arg1	modulus					913:919	the mean Young's modulus	896:919	the mean Young's modulus	896:919	Single fiber tensile tests revealed that the DSSFs exhibited the mean Young's modulus and tensile strength of 13.54 GPa and 633.87 MPa respectively.					
33091474	2	6	dep	Derris	272:277	arg1	scandens					279:286	scandens	279:286	scandens	279:286	With this objective, a comprehensive characterization of Derris scandens stem fibers (DSSFs) was carried out.					
33091474	4	7	theme	%	561:561	arg1	contents					542:549	the cellulose, hemicellulose, and lignin contents	501:549	the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively	501:599	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33091474	8	8	theme	thermogravimetric	1082:1098	arg1	analysis					1100:1107	thermogravimetric analysis	1082:1107	thermogravimetric analysis	1082:1107	Furthermore, the extracted fibers were found to be thermally stable up to 230 °C, as confirmed by thermogravimetric analysis.					
33091474	9	9	theme	natural	1231:1237	arg1	fibers					1239:1244	existing natural fibers	1222:1244	existing natural fibers	1222:1244	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	7	10	theme	Young	905:909	arg1	modulus					913:919	the mean Young's modulus	896:919	the mean Young's modulus	896:919	Single fiber tensile tests revealed that the DSSFs exhibited the mean Young's modulus and tensile strength of 13.54 GPa and 633.87 MPa respectively.					
33091474	3	11	with	fibers	366:371	arg1	structure					410:418	a traditional hierarchical cell structure	378:418	a traditional hierarchical cell structure	378:418	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	4	12	theme	%	571:571	arg1	contents					542:549	the cellulose, hemicellulose, and lignin contents	501:549	the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively	501:599	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33091474	5	13	theme	11.92 nm	693:700	arg1	size					685:688	a size	683:688	a size of 11.92 nm	683:700	Further analysis with XRD confirmed the presence of crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%.					
33091474	2	14	theme	Derris	272:277	arg1	fibers					293:298	Derris scandens stem fibers	272:298	Derris scandens stem fibers (DSSFs)	272:306	With this objective, a comprehensive characterization of Derris scandens stem fibers (DSSFs) was carried out.					
33091474	2	14	theme	Derris	272:277	arg1	DSSFs					301:305	DSSFs	301:305	DSSFs	301:305	With this objective, a comprehensive characterization of Derris scandens stem fibers (DSSFs) was carried out.					
33091474	4	15	theme	11.6 wt	564:570	arg1	%					571:571	11.6 wt%	564:571	11.6 wt%	564:571	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33091474	9	16	contain	have	1180:1183	arg2	properties					1189:1198	the properties	1185:1198	the properties comparable to that of existing natural fibers	1185:1244	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	9	16	contain	have	1180:1183	arg1	fibers					1114:1119	The fibers	1110:1119	The fibers extracted from the stem of medicinal plant Derris scandens	1110:1178	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	6	17	theme	AFM	748:750	arg1	studies					752:758	SEM and AFM studies	740:758	SEM and AFM studies	740:758	SEM and AFM studies show that these fibers are porous, and the average roughness is 105.95 nm.					
33091474	1	18	theme	harmful	154:160	arg1	fibers					172:177	the harmful synthetic fibers	150:177	the harmful synthetic fibers	150:177	The present study aims to identify a potential substitute for the harmful synthetic fibers in the field of polymer composites.					
33091474	9	19	theme	polymer	1349:1355	arg1	composites					1364:1373	polymer matrix composites	1349:1373	polymer matrix composites	1349:1373	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	4	20	theme	compositional	468:480	arg1	analysis					482:489	The chemical compositional analysis	455:489	The chemical compositional analysis	455:489	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33091474	3	21	theme	traditional	380:390	arg1	structure					410:418	a traditional hierarchical cell structure	378:418	a traditional hierarchical cell structure	378:418	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	3	22	located	found	424:428	arg2	presence					329:336	The presence	325:336	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure	325:418	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	3	22	located	found	424:428	arg1	study					448:452	the anatomical study	433:452	the anatomical study	433:452	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	1	23	theme	synthetic	162:170	arg1	fibers					172:177	the harmful synthetic fibers	150:177	the harmful synthetic fibers	150:177	The present study aims to identify a potential substitute for the harmful synthetic fibers in the field of polymer composites.					
33091474	3	24	theme	gelatinous	355:364	arg1	fibers					366:371	high strength gelatinous fibers	341:371	high strength gelatinous fibers with a traditional hierarchical cell structure	341:418	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	4	25	theme	chemical	459:466	arg1	analysis					482:489	The chemical compositional analysis	455:489	The chemical compositional analysis	455:489	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33091474	6	26	theme	SEM	740:742	arg1	studies					752:758	SEM and AFM studies	740:758	SEM and AFM studies	740:758	SEM and AFM studies show that these fibers are porous, and the average roughness is 105.95 nm.					
33091474	3	27	theme	cell	405:408	arg1	structure					410:418	a traditional hierarchical cell structure	378:418	a traditional hierarchical cell structure	378:418	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	3	28	theme	fibers	366:371	arg1	presence					329:336	The presence	325:336	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure	325:418	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	5	29	theme	Further	602:608	arg1	analysis					610:617	Further analysis	602:617	Further analysis with XRD	602:626	Further analysis with XRD confirmed the presence of crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%.					
33091474	3	30	theme	high	341:344	arg1	fibers					366:371	high strength gelatinous fibers	341:371	high strength gelatinous fibers with a traditional hierarchical cell structure	341:418	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	4	31	theme	lignin	535:540	arg1	contents					542:549	the cellulose, hemicellulose, and lignin contents	501:549	the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively	501:599	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33091474	5	32	theme	cellulose	666:674	arg1	presence					642:649	the presence	638:649	the presence of crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%	638:737	Further analysis with XRD confirmed the presence of crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%.					
33091474	9	33	theme	matrix	1357:1362	arg1	composites					1364:1373	polymer matrix composites	1349:1373	polymer matrix composites	1349:1373	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	1	34	dep	potential	125:133	arg1	substitute					135:144	substitute	135:144	substitute for the harmful synthetic fibers in the field of polymer composites	135:212	The present study aims to identify a potential substitute for the harmful synthetic fibers in the field of polymer composites.					
33091474	3	35	theme	anatomical	437:446	arg1	study					448:452	the anatomical study	433:452	the anatomical study	433:452	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	3	36	theme	strength	346:353	arg1	fibers					366:371	high strength gelatinous fibers	341:371	high strength gelatinous fibers with a traditional hierarchical cell structure	341:418	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	4	37	theme	63.3 wt	554:560	arg1	%					561:561	63.3 wt%	554:561	63.3 wt%	554:561	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33091474	6	38	theme	average	803:809	arg1	105.95 nm					824:832	105.95 nm	824:832	105.95 nm	824:832	SEM and AFM studies show that these fibers are porous, and the average roughness is 105.95 nm.					
33091474	6	38	theme	average	803:809	arg1	roughness					811:819	the average roughness	799:819	the average roughness	799:819	SEM and AFM studies show that these fibers are porous, and the average roughness is 105.95 nm.					
33091474	2	39	theme	stem	288:291	arg1	fibers					293:298	Derris scandens stem fibers	272:298	Derris scandens stem fibers (DSSFs)	272:306	With this objective, a comprehensive characterization of Derris scandens stem fibers (DSSFs) was carried out.					
33091474	2	39	theme	stem	288:291	arg1	DSSFs					301:305	DSSFs	301:305	DSSFs	301:305	With this objective, a comprehensive characterization of Derris scandens stem fibers (DSSFs) was carried out.					
33091474	0	40	theme	cellulosic	34:43	arg1	fiber					45:49	a new natural cellulosic fiber	20:49	a new natural cellulosic fiber extracted from Derris	20:71	Characterization of a new natural cellulosic fiber extracted from Derris scandens stem.					
33091474	9	41	theme	Derris	1164:1169	arg1	scandens					1171:1178	medicinal plant Derris scandens	1148:1178	medicinal plant Derris scandens	1148:1178	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	1	42	theme	present	92:98	arg1	study					100:104	The present study	88:104	The present study	88:104	The present study aims to identify a potential substitute for the harmful synthetic fibers in the field of polymer composites.					
33091474	0	43	theme	natural	26:32	arg1	fiber					45:49	a new natural cellulosic fiber	20:49	a new natural cellulosic fiber extracted from Derris	20:71	Characterization of a new natural cellulosic fiber extracted from Derris scandens stem.					
33091474	4	44	theme	hemicellulose	516:528	arg1	contents					542:549	the cellulose, hemicellulose, and lignin contents	501:549	the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively	501:599	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33091474	3	45	theme	hierarchical	392:403	arg1	structure					410:418	a traditional hierarchical cell structure	378:418	a traditional hierarchical cell structure	378:418	The presence of high strength gelatinous fibers with a traditional hierarchical cell structure was found in the anatomical study.					
33091474	9	46	theme	scandens	1171:1178	arg1	stem					1140:1143	the stem	1136:1143	the stem of medicinal plant Derris scandens	1136:1178	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	0	47	theme	new	22:24	arg1	fiber					45:49	a new natural cellulosic fiber	20:49	a new natural cellulosic fiber extracted from Derris	20:71	Characterization of a new natural cellulosic fiber extracted from Derris scandens stem.					
33091474	7	48	theme	13.54 GPa	945:953	arg1	modulus					913:919	the mean Young's modulus	896:919	the mean Young's modulus	896:919	Single fiber tensile tests revealed that the DSSFs exhibited the mean Young's modulus and tensile strength of 13.54 GPa and 633.87 MPa respectively.					
33091474	7	48	theme	13.54 GPa	945:953	arg1	strength					933:940	tensile strength	925:940	tensile strength of 13.54 GPa and 633.87 MPa	925:968	Single fiber tensile tests revealed that the DSSFs exhibited the mean Young's modulus and tensile strength of 13.54 GPa and 633.87 MPa respectively.					
33091474	1	49	theme	polymer	195:201	arg1	composites					203:212	polymer composites	195:212	polymer composites	195:212	The present study aims to identify a potential substitute for the harmful synthetic fibers in the field of polymer composites.					
33091474	9	50	theme	medicinal	1148:1156	arg1	scandens					1171:1178	medicinal plant Derris scandens	1148:1178	medicinal plant Derris scandens	1148:1178	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	0	51	theme	fiber	45:49	arg1	Characterization					0:15	Characterization	0:15	Characterization of a new natural cellulosic fiber extracted from Derris	0:71	Characterization of a new natural cellulosic fiber extracted from Derris scandens stem.					
33091474	9	52	from	fibers	1339:1344	arg1	composites					1364:1373	polymer matrix composites	1349:1373	polymer matrix composites	1349:1373	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	7	53	theme	tensile	848:854	arg1	tests					856:860	Single fiber tensile tests	835:860	Single fiber tensile tests	835:860	Single fiber tensile tests revealed that the DSSFs exhibited the mean Young's modulus and tensile strength of 13.54 GPa and 633.87 MPa respectively.					
33091474	9	54	theme	promising	1286:1294	arg1	agent					1308:1312	a highly promising reinforcing agent	1277:1312	a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites	1277:1373	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	7	55	theme	633.87 MPa	959:968	arg1	modulus					913:919	the mean Young's modulus	896:919	the mean Young's modulus	896:919	Single fiber tensile tests revealed that the DSSFs exhibited the mean Young's modulus and tensile strength of 13.54 GPa and 633.87 MPa respectively.					
33091474	7	55	theme	633.87 MPa	959:968	arg1	strength					933:940	tensile strength	925:940	tensile strength of 13.54 GPa and 633.87 MPa	925:968	Single fiber tensile tests revealed that the DSSFs exhibited the mean Young's modulus and tensile strength of 13.54 GPa and 633.87 MPa respectively.					
33091474	2	56	theme	comprehensive	238:250	arg1	characterization					252:267	a comprehensive characterization	236:267	a comprehensive characterization of Derris scandens stem fibers (DSSFs)	236:306	With this objective, a comprehensive characterization of Derris scandens stem fibers (DSSFs) was carried out.					
33091474	2	57	theme	fibers	293:298	arg1	characterization					252:267	a comprehensive characterization	236:267	a comprehensive characterization of Derris scandens stem fibers (DSSFs)	236:306	With this objective, a comprehensive characterization of Derris scandens stem fibers (DSSFs) was carried out.					
33091474	1	58	theme	composites	203:212	arg1	field					186:190	the field	182:190	the field of polymer composites	182:212	The present study aims to identify a potential substitute for the harmful synthetic fibers in the field of polymer composites.					
33091474	5	59	theme	%	737:737	arg1	index					723:727	a crystallinity index	707:727	a crystallinity index of 58.15%	707:737	Further analysis with XRD confirmed the presence of crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%.					
33091474	9	60	theme	comparable	1200:1209	arg1	properties					1189:1198	the properties	1185:1198	the properties comparable to that of existing natural fibers	1185:1244	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	9	61	theme	alternative	1314:1324	arg1	agent					1308:1312	a highly promising reinforcing agent	1277:1312	a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites	1277:1373	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	8	62	dep	230 °C	1058:1063	arg1	up					1052:1053	up	1052:1053	up	1052:1053	Furthermore, the extracted fibers were found to be thermally stable up to 230 °C, as confirmed by thermogravimetric analysis.					
33091474	7	63	theme	Single	835:840	arg1	tests					856:860	Single fiber tensile tests	835:860	Single fiber tensile tests	835:860	Single fiber tensile tests revealed that the DSSFs exhibited the mean Young's modulus and tensile strength of 13.54 GPa and 633.87 MPa respectively.					
33091474	5	64	theme	crystallinity	709:721	arg1	index					723:727	a crystallinity index	707:727	a crystallinity index of 58.15%	707:737	Further analysis with XRD confirmed the presence of crystalline cellulose having a size of 11.92 nm with a crystallinity index of 58.15%.					
33091474	8	65	theme	extracted	1001:1009	arg1	stable					1045:1050	stable	1045:1050	stable	1045:1050	Furthermore, the extracted fibers were found to be thermally stable up to 230 °C, as confirmed by thermogravimetric analysis.					
33091474	8	65	theme	extracted	1001:1009	arg1	fibers					1011:1016	the extracted fibers	997:1016	the extracted fibers	997:1016	Furthermore, the extracted fibers were found to be thermally stable up to 230 °C, as confirmed by thermogravimetric analysis.					
33091474	9	66	theme	plant	1158:1162	arg1	scandens					1171:1178	medicinal plant Derris scandens	1148:1178	medicinal plant Derris scandens	1148:1178	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	7	67	theme	tensile	925:931	arg1	strength					933:940	tensile strength	925:940	tensile strength of 13.54 GPa and 633.87 MPa	925:968	Single fiber tensile tests revealed that the DSSFs exhibited the mean Young's modulus and tensile strength of 13.54 GPa and 633.87 MPa respectively.					
33091474	7	68	theme	fiber	842:846	arg1	tests					856:860	Single fiber tensile tests	835:860	Single fiber tensile tests	835:860	Single fiber tensile tests revealed that the DSSFs exhibited the mean Young's modulus and tensile strength of 13.54 GPa and 633.87 MPa respectively.					
33091474	4	69	theme	%	585:585	arg1	contents					542:549	the cellulose, hemicellulose, and lignin contents	501:549	the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively	501:599	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33091474	9	70	theme	existing	1222:1229	arg1	fibers					1239:1244	existing natural fibers	1222:1244	existing natural fibers	1222:1244	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	9	71	theme	reinforcing	1296:1306	arg1	agent					1308:1312	a highly promising reinforcing agent	1277:1312	a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites	1277:1373	The fibers extracted from the stem of medicinal plant Derris scandens have the properties comparable to that of existing natural fibers, thus, suggesting it to use as a highly promising reinforcing agent alternative to synthetic fibers in polymer matrix composites.					
33091474	4	72	theme	15.3 wt	578:584	arg1	%					585:585	15.3 wt%	578:585	15.3 wt%	578:585	The chemical compositional analysis estimated the cellulose, hemicellulose, and lignin contents of 63.3 wt%, 11.6 wt%, and 15.3 wt%, respectively.					
33877517	2	0	theme	halophilic	117:126	arg1	archaea					128:134	halophilic archaea	117:134	halophilic archaea	117:134	OBJECTIVES Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles.					
33877517	8	1	theme	hydrogel	873:880	arg1	composite					882:890	the hydrogel composite	869:890	the hydrogel composite	869:890	The cell lysate was injected into different metal ion (or H+) solutions to obtain the hydrogel composite.					
33877517	4	2	attach	isolated	457:464	arg2	which					447:451	which	447:451	which	447:451	(H.e.) which was isolated from a saline lake in Inner Mongolia, China.					
33877517	4	2	attach	isolated	457:464	arg1	lake					480:483	a saline lake	471:483	a saline lake in Inner Mongolia, China	471:508	(H.e.) which was isolated from a saline lake in Inner Mongolia, China.					
33877517	12	3	theme	H.e.	1424:1427	arg1	composite					1438:1446	La3+-induced H.e. hydrogel composite	1411:1446	La3+-induced H.e. hydrogel composite	1411:1446	Potential application of the hydrogel as an adsorbing material was explored using La3+-induced H.e. hydrogel composite.					
33877517	3	4	theme	simple	322:327	arg1	method					329:334	an simple method	319:334	an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp	319:437	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	4	5	from	lake	480:483	arg1	China					504:508	China	504:508	China	504:508	(H.e.) which was isolated from a saline lake in Inner Mongolia, China.					
33877517	6	6	dep	sp	654:655	arg1	RESULTS					627:633	RESULTS	627:633	RESULTS	627:633	RESULTS Halorubrum ejinoor sp.					
33877517	14	7	theme	cost	1600:1603	arg1	effectiveness					1605:1617	cost effectiveness	1600:1617	cost effectiveness	1600:1617	CONCLUSIONS The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material.					
33877517	7	8	theme	cell	665:668	arg1	lysate					670:675	(H.e.) cell lysate	658:675	(H.e.) cell lysate	658:675	(H.e.) cell lysate was firstly prepared by adding pure water onto the H.e. cell pellet, followed by a short incubation at 60 °C.					
33877517	3	9	theme	hydrogel	349:356	arg1	composite					358:366	a hydrogel composite	347:366	a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp	347:437	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	10	10	from	polysaccharides	1055:1069	arg1	lysate					1094:1099	the H.e. cell lysate	1080:1099	the H.e. cell lysate	1080:1099	DNA and extracellular polysaccharides (EPS) in the H.e. cell lysate were found to be responsible for the gelation reaction.					
33877517	8	11	theme	cell	791:794	arg1	lysate					796:801	The cell lysate	787:801	The cell lysate	787:801	The cell lysate was injected into different metal ion (or H+) solutions to obtain the hydrogel composite.					
33877517	14	12	dep	CONCLUSIONS	1569:1579	arg1	make					1651:1654	make	1651:1654	might make H.e. hydrogel a promising adsorbing material.	1645:1700	CONCLUSIONS The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material.					
33877517	11	13	theme	metal	1221:1225	arg1	ions					1227:1230	metal ions	1221:1230	metal ions (or H+)	1221:1238	These results suggest that DNA and EPS should be crosslinked by metal ions (or H+) and form a networked structure in which the metal ion (or H+) serves as an anchor point.					
33877517	11	13	theme	metal	1221:1225	arg1	H+					1236:1237	H+	1236:1237	H+	1236:1237	These results suggest that DNA and EPS should be crosslinked by metal ions (or H+) and form a networked structure in which the metal ion (or H+) serves as an anchor point.					
33877517	2	14	theme	natural	148:154	arg1	environments					156:167	natural environments	148:167	natural environments	148:167	OBJECTIVES Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles.					
33877517	14	15	dep	hydrogel	1661:1668	arg1	material					1692:1699	a promising adsorbing material	1670:1699	H.e. hydrogel a promising adsorbing material.	1656:1700	CONCLUSIONS The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material.					
33877517	5	16	theme	hydrogel	578:585	arg1	mechanism					534:542	formation mechanism	524:542	formation mechanism	524:542	Furthermore, formation mechanism and potential applications of the hydrogel as an adsorbing material are discussed.					
33877517	5	16	theme	hydrogel	578:585	arg1	applications					558:569	potential applications	548:569	potential applications of the hydrogel as an adsorbing material	548:610	Furthermore, formation mechanism and potential applications of the hydrogel as an adsorbing material are discussed.					
33877517	1	17	theme	cell	59:62	arg1	lysate					64:69	cell lysate	59:69	cell lysate	59:69	cell lysate as an adsorbing material.					
33877517	12	18	theme	La3+-induced	1411:1422	arg1	composite					1438:1446	La3+-induced H.e. hydrogel composite	1411:1446	La3+-induced H.e. hydrogel composite	1411:1446	Potential application of the hydrogel as an adsorbing material was explored using La3+-induced H.e. hydrogel composite.					
33877517	10	19	theme	cell	1089:1092	arg1	lysate					1094:1099	the H.e. cell lysate	1080:1099	the H.e. cell lysate	1080:1099	DNA and extracellular polysaccharides (EPS) in the H.e. cell lysate were found to be responsible for the gelation reaction.					
33877517	13	20	theme	acridine	1552:1559	arg1	orange					1561:1566	acridine orange	1552:1566	acridine orange	1552:1566	The hydrogel composite can adsorb the fluoride, phosphate and DNA-binding carcinogenic agents, such as acridine orange.					
33877517	3	21	theme	ejinoor	428:434	arg1	sp					436:437	Halorubrum ejinoor sp	417:437	Halorubrum ejinoor sp	417:437	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	3	21	theme	ejinoor	428:434	arg1	archaea					408:414	a halophilic archaea	395:414	a halophilic archaea	395:414	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	0	22	theme	Halorubrum	36:45	arg1	Halorubrum					36:45	Halorubrum	36:45	Halorubrum	36:45	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	0	22	theme	Halorubrum	36:45	arg1	lanthanum					22:30	lanthanum	22:30	lanthanum	22:30	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	0	22	theme	Halorubrum	36:45	arg1	composite					9:17	Hydrogel composite	0:17	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.	0:57	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	11	23	theme	anchor	1315:1320	arg1	ion					1290:1292	the metal ion	1280:1292	the metal ion (or H+)	1280:1300	These results suggest that DNA and EPS should be crosslinked by metal ions (or H+) and form a networked structure in which the metal ion (or H+) serves as an anchor point.					
33877517	11	23	theme	anchor	1315:1320	arg1	point					1322:1326	an anchor point	1312:1326	an anchor point	1312:1326	These results suggest that DNA and EPS should be crosslinked by metal ions (or H+) and form a networked structure in which the metal ion (or H+) serves as an anchor point.					
33877517	13	24	theme	carcinogenic	1523:1534	arg1	orange					1561:1566	acridine orange	1552:1566	acridine orange	1552:1566	The hydrogel composite can adsorb the fluoride, phosphate and DNA-binding carcinogenic agents, such as acridine orange.					
33877517	13	24	theme	carcinogenic	1523:1534	arg1	agents					1536:1541	DNA-binding carcinogenic agents	1511:1541	DNA-binding carcinogenic agents	1511:1541	The hydrogel composite can adsorb the fluoride, phosphate and DNA-binding carcinogenic agents, such as acridine orange.					
33877517	8	25	theme	different	821:829	arg1	solutions					849:857	different metal ion (or H+) solutions	821:857	different metal ion (or H+) solutions	821:857	The cell lysate was injected into different metal ion (or H+) solutions to obtain the hydrogel composite.					
33877517	3	26	theme	cell	380:383	arg1	lysate					385:390	crude cell lysate	374:390	crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp	374:437	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	9	27	theme	cell	969:972	arg1	lysate					974:979	the cell lysate	965:979	the cell lysate	965:979	It was observed that H+, Fe3+, La3+, Cu2+, and Ca2+ induced gelation of the cell lysate, while Fe2+, Co2+, Ni2+, Mg2+, Na+, and K+ did not.					
33877517	14	28	theme	adsorbing	1682:1690	arg1	material					1692:1699	a promising adsorbing material	1670:1699	H.e. hydrogel a promising adsorbing material.	1656:1700	CONCLUSIONS The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material.					
33877517	0	29	theme	Hydrogel	0:7	arg1	Halorubrum					36:45	Halorubrum	36:45	Halorubrum	36:45	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	0	29	theme	Hydrogel	0:7	arg1	lanthanum					22:30	lanthanum	22:30	lanthanum	22:30	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	0	29	theme	Hydrogel	0:7	arg1	composite					9:17	Hydrogel composite	0:17	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.	0:57	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	12	30	theme	hydrogel	1358:1365	arg1	application					1339:1349	Potential application	1329:1349	Potential application of the hydrogel as an adsorbing material	1329:1390	Potential application of the hydrogel as an adsorbing material was explored using La3+-induced H.e. hydrogel composite.					
33877517	13	31	theme	hydrogel	1453:1460	arg1	composite					1462:1470	The hydrogel composite	1449:1470	The hydrogel composite	1449:1470	The hydrogel composite can adsorb the fluoride, phosphate and DNA-binding carcinogenic agents, such as acridine orange.					
33877517	5	32	theme	potential	548:556	arg1	applications					558:569	potential applications	548:569	potential applications of the hydrogel as an adsorbing material	548:610	Furthermore, formation mechanism and potential applications of the hydrogel as an adsorbing material are discussed.					
33877517	1	33	theme	adsorbing	77:85	arg1	material					87:94	an adsorbing material	74:94	an adsorbing material	74:94	cell lysate as an adsorbing material.					
33877517	8	34	theme	ion	837:839	arg1	solutions					849:857	different metal ion (or H+) solutions	821:857	different metal ion (or H+) solutions	821:857	The cell lysate was injected into different metal ion (or H+) solutions to obtain the hydrogel composite.					
33877517	3	35	theme	Halorubrum	417:426	arg1	sp					436:437	Halorubrum ejinoor sp	417:437	Halorubrum ejinoor sp	417:437	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	3	35	theme	Halorubrum	417:426	arg1	archaea					408:414	a halophilic archaea	395:414	a halophilic archaea	395:414	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	0	36	theme	lanthanum	22:30	arg1	Halorubrum					36:45	Halorubrum	36:45	Halorubrum	36:45	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	0	36	theme	lanthanum	22:30	arg1	lanthanum					22:30	lanthanum	22:30	lanthanum	22:30	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	0	36	theme	lanthanum	22:30	arg1	composite					9:17	Hydrogel composite	0:17	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.	0:57	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	4	37	dep	isolated	457:464	arg1	H.e.					441:444	H.e.	441:444	H.e.	441:444	(H.e.) which was isolated from a saline lake in Inner Mongolia, China.					
33877517	7	38	from	60 °C	780:784	arg1	incubation					766:775	a short incubation	758:775	a short incubation at 60 °C	758:784	(H.e.) cell lysate was firstly prepared by adding pure water onto the H.e. cell pellet, followed by a short incubation at 60 °C.					
33877517	2	39	from	rich	140:143	arg1	environments					156:167	natural environments	148:167	natural environments	148:167	OBJECTIVES Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles.					
33877517	8	40	theme	metal	831:835	arg1	ion					837:839	metal ion	831:839	different metal ion (or H+) solutions	821:857	The cell lysate was injected into different metal ion (or H+) solutions to obtain the hydrogel composite.					
33877517	8	40	theme	metal	831:835	arg1	H+					845:846	H+	845:846	H+	845:846	The cell lysate was injected into different metal ion (or H+) solutions to obtain the hydrogel composite.					
33877517	3	41	theme	crude	374:378	arg1	lysate					385:390	crude cell lysate	374:390	crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp	374:437	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	2	42	theme	biotechnological	176:191	arg1	applications					193:204	their biotechnological applications	170:204	their biotechnological applications	170:204	OBJECTIVES Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles.					
33877517	7	43	theme	short	760:764	arg1	incubation					766:775	a short incubation	758:775	a short incubation at 60 °C	758:784	(H.e.) cell lysate was firstly prepared by adding pure water onto the H.e. cell pellet, followed by a short incubation at 60 °C.					
33877517	9	44	theme	lysate	974:979	arg1	gelation					953:960	gelation	953:960	gelation of the cell lysate	953:979	It was observed that H+, Fe3+, La3+, Cu2+, and Ca2+ induced gelation of the cell lysate, while Fe2+, Co2+, Ni2+, Mg2+, Na+, and K+ did not.					
33877517	3	45	theme	halophilic	397:406	arg1	sp					436:437	Halorubrum ejinoor sp	417:437	Halorubrum ejinoor sp	417:437	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	3	45	theme	halophilic	397:406	arg1	archaea					408:414	a halophilic archaea	395:414	a halophilic archaea	395:414	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	6	46	theme	ejinoor	646:652	arg1	sp					654:655	Halorubrum ejinoor sp	635:655	Halorubrum ejinoor sp	635:655	RESULTS Halorubrum ejinoor sp.					
33877517	14	47	theme	H.e.	1656:1659	arg1	hydrogel					1661:1668	H.e. hydrogel	1656:1668	H.e. hydrogel a promising adsorbing material.	1656:1700	CONCLUSIONS The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material.					
33877517	3	48	theme	archaea	408:414	arg1	lysate					385:390	crude cell lysate	374:390	crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp	374:437	This study presents an simple method to prepare a hydrogel composite using crude cell lysate of a halophilic archaea, Halorubrum ejinoor sp.					
33877517	7	49	theme	cell	733:736	arg1	pellet					738:743	the H.e. cell pellet	724:743	the H.e. cell pellet	724:743	(H.e.) cell lysate was firstly prepared by adding pure water onto the H.e. cell pellet, followed by a short incubation at 60 °C.					
33877517	7	50	theme	H.e.	659:662	arg1	lysate					670:675	(H.e.) cell lysate	658:675	(H.e.) cell lysate	658:675	(H.e.) cell lysate was firstly prepared by adding pure water onto the H.e. cell pellet, followed by a short incubation at 60 °C.					
33877517	12	51	theme	Potential	1329:1337	arg1	application					1339:1349	Potential application	1329:1349	Potential application of the hydrogel as an adsorbing material	1329:1390	Potential application of the hydrogel as an adsorbing material was explored using La3+-induced H.e. hydrogel composite.					
33877517	15	52	theme	haloarchaea	1764:1774	arg1	applications					1748:1759	the technical applications	1734:1759	the technical applications of haloarchaea	1734:1774	This work is expected to expand the technical applications of haloarchaea.					
33877517	1	53	dep	lysate	64:69	arg1	material					87:94	an adsorbing material	74:94	an adsorbing material	74:94	cell lysate as an adsorbing material.					
33877517	0	54	theme	ejinoor	47:53	arg1	sp					55:56	ejinoor sp	47:56	ejinoor sp	47:56	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	14	55	dep	simplicity	1585:1594	arg1	The					1581:1583	The	1581:1583	The	1581:1583	CONCLUSIONS The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material.					
33877517	5	56	theme	adsorbing	593:601	arg1	material					603:610	an adsorbing material	590:610	an adsorbing material	590:610	Furthermore, formation mechanism and potential applications of the hydrogel as an adsorbing material are discussed.					
33877517	10	57	theme	gelation	1138:1145	arg1	reaction					1147:1154	the gelation reaction	1134:1154	the gelation reaction	1134:1154	DNA and extracellular polysaccharides (EPS) in the H.e. cell lysate were found to be responsible for the gelation reaction.					
33877517	14	58	theme	method	1638:1643	arg1	effectiveness					1605:1617	cost effectiveness	1600:1617	cost effectiveness	1600:1617	CONCLUSIONS The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material.					
33877517	14	58	theme	method	1638:1643	arg1	simplicity					1585:1594	simplicity	1585:1594	simplicity	1585:1594	CONCLUSIONS The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material.					
33877517	6	59	theme	Halorubrum	635:644	arg1	sp					654:655	Halorubrum ejinoor sp	635:655	Halorubrum ejinoor sp	635:655	RESULTS Halorubrum ejinoor sp.					
33877517	7	60	theme	H.e.	728:731	arg1	pellet					738:743	the H.e. cell pellet	724:743	the H.e. cell pellet	724:743	(H.e.) cell lysate was firstly prepared by adding pure water onto the H.e. cell pellet, followed by a short incubation at 60 °C.					
33877517	0	61	dep	lanthanum	22:30	arg1	sp					55:56	ejinoor sp	47:56	ejinoor sp	47:56	Hydrogel composite of lanthanum and Halorubrum ejinoor sp.					
33877517	5	62	theme	formation	524:532	arg1	mechanism					534:542	formation mechanism	524:542	formation mechanism	524:542	Furthermore, formation mechanism and potential applications of the hydrogel as an adsorbing material are discussed.					
33877517	14	63	theme	preparation	1626:1636	arg1	method					1638:1643	the preparation method	1622:1643	the preparation method	1622:1643	CONCLUSIONS The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material.					
33877517	14	64	theme	promising	1672:1680	arg1	material					1692:1699	a promising adsorbing material	1670:1699	H.e. hydrogel a promising adsorbing material.	1656:1700	CONCLUSIONS The simplicity and cost effectiveness of the preparation method might make H.e. hydrogel a promising adsorbing material.					
33877517	10	65	from	DNA	1033:1035	arg1	lysate					1094:1099	the H.e. cell lysate	1080:1099	the H.e. cell lysate	1080:1099	DNA and extracellular polysaccharides (EPS) in the H.e. cell lysate were found to be responsible for the gelation reaction.					
33877517	15	66	theme	technical	1738:1746	arg1	applications					1748:1759	the technical applications	1734:1759	the technical applications of haloarchaea	1734:1774	This work is expected to expand the technical applications of haloarchaea.					
33877517	12	67	theme	adsorbing	1373:1381	arg1	material					1383:1390	an adsorbing material	1370:1390	an adsorbing material	1370:1390	Potential application of the hydrogel as an adsorbing material was explored using La3+-induced H.e. hydrogel composite.					
33877517	11	68	theme	networked	1251:1259	arg1	structure					1261:1269	a networked structure	1249:1269	a networked structure in which the metal ion (or H+) serves as an anchor point	1249:1326	These results suggest that DNA and EPS should be crosslinked by metal ions (or H+) and form a networked structure in which the metal ion (or H+) serves as an anchor point.					
33877517	12	69	theme	hydrogel	1429:1436	arg1	composite					1438:1446	La3+-induced H.e. hydrogel composite	1411:1446	La3+-induced H.e. hydrogel composite	1411:1446	Potential application of the hydrogel as an adsorbing material was explored using La3+-induced H.e. hydrogel composite.					
33877517	11	70	theme	metal	1284:1288	arg1	ion					1290:1292	the metal ion	1280:1292	the metal ion (or H+)	1280:1300	These results suggest that DNA and EPS should be crosslinked by metal ions (or H+) and form a networked structure in which the metal ion (or H+) serves as an anchor point.					
33877517	11	70	theme	metal	1284:1288	arg1	H+					1298:1299	H+	1298:1299	H+	1298:1299	These results suggest that DNA and EPS should be crosslinked by metal ions (or H+) and form a networked structure in which the metal ion (or H+) serves as an anchor point.					
33877517	11	70	theme	metal	1284:1288	arg1	point					1322:1326	an anchor point	1312:1326	an anchor point	1312:1326	These results suggest that DNA and EPS should be crosslinked by metal ions (or H+) and form a networked structure in which the metal ion (or H+) serves as an anchor point.					
33877517	10	71	theme	extracellular	1041:1053	arg1	polysaccharides					1055:1069	extracellular polysaccharides	1041:1069	extracellular polysaccharides	1041:1069	DNA and extracellular polysaccharides (EPS) in the H.e. cell lysate were found to be responsible for the gelation reaction.					
33877517	2	72	from	environments	156:167	arg1	rich					140:143	rich	140:143	rich	140:143	OBJECTIVES Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles.					
33877517	4	73	theme	saline	473:478	arg1	lake					480:483	a saline lake	471:483	a saline lake in Inner Mongolia, China	471:508	(H.e.) which was isolated from a saline lake in Inner Mongolia, China.					
33877517	2	74	theme	other	239:243	arg1	extremophiles					245:257	other extremophiles	239:257	other extremophiles	239:257	OBJECTIVES Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles.					
33877517	2	74	theme	other	239:243	arg1	alkaliphiles					285:296	alkaliphiles	285:296	alkaliphiles	285:296	OBJECTIVES Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles.					
33877517	2	74	theme	other	239:243	arg1	thermophiles					268:279	thermophiles	268:279	thermophiles	268:279	OBJECTIVES Although halophilic archaea are rich in natural environments, their biotechnological applications are not as prevalent as those of other extremophiles, such as thermophiles and alkaliphiles.					
33877517	13	75	theme	DNA-binding	1511:1521	arg1	orange					1561:1566	acridine orange	1552:1566	acridine orange	1552:1566	The hydrogel composite can adsorb the fluoride, phosphate and DNA-binding carcinogenic agents, such as acridine orange.					
33877517	13	75	theme	DNA-binding	1511:1521	arg1	agents					1536:1541	DNA-binding carcinogenic agents	1511:1541	DNA-binding carcinogenic agents	1511:1541	The hydrogel composite can adsorb the fluoride, phosphate and DNA-binding carcinogenic agents, such as acridine orange.					
33877517	10	76	theme	H.e.	1084:1087	arg1	lysate					1094:1099	the H.e. cell lysate	1080:1099	the H.e. cell lysate	1080:1099	DNA and extracellular polysaccharides (EPS) in the H.e. cell lysate were found to be responsible for the gelation reaction.					
33877517	7	77	theme	pure	708:711	arg1	water					713:717	pure water	708:717	pure water	708:717	(H.e.) cell lysate was firstly prepared by adding pure water onto the H.e. cell pellet, followed by a short incubation at 60 °C.					
32397299	5	0	theme	uronic	734:739	arg1	acid					741:744	uronic acid	734:744	uronic acid	734:744	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	10	1	theme	excellent	1326:1334	arg1	activities					1369:1378	excellent antihypertensive and antioxidant activities	1326:1378	excellent antihypertensive and antioxidant activities	1326:1378	Finally, the results revealed that EAP displayed excellent antihypertensive and antioxidant activities.					
32397299	11	2	theme	promising	1404:1412	arg1	source					1422:1427	a promising natural source	1402:1427	a promising natural source of food additives, antioxidants, and antihypertensive agents	1402:1488	Overall, EAP present a promising natural source of food additives, antioxidants, and antihypertensive agents.					
32397299	2	3	theme	liquid/solid	354:365	arg1	ratio					367:371	a liquid/solid ratio	352:371	a liquid/solid ratio of 5 in a water bath at 90 °C	352:401	EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C.					
32397299	6	4	theme	functional	897:906	arg1	C-H					921:923	C-H	921:923	C-H	921:923	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	6	4	theme	functional	897:906	arg1	O-H					930:932	O-H	930:932	O-H	930:932	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	6	4	theme	functional	897:906	arg1	C=O					916:918	C=O	916:918	C=O	916:918	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	6	4	theme	functional	897:906	arg1	groups					908:913	three functional groups	891:913	three functional groups: C=O, C-H, and O-H	891:932	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	0	5	theme	Functional	85:94	arg1	Properties					96:105	Functional Properties	85:105	Functional Properties	85:105	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	5	6	theme	%	804:804	arg1	%					812:812	6.56% ± 0.36%	800:812	6.56% ± 0.36%	800:812	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	5	7	theme	±	806:806	arg1	%					812:812	6.56% ± 0.36%	800:812	6.56% ± 0.36%	800:812	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	11	8	theme	food	1432:1435	arg1	additives					1437:1445	food additives	1432:1445	food additives	1432:1445	Overall, EAP present a promising natural source of food additives, antioxidants, and antihypertensive agents.					
32397299	7	9	theme	high	979:982	arg1	particles					993:1001	numerous high porosity particles	970:1001	numerous high porosity particles	970:1001	SEM images showed that EAP present numerous high porosity particles.					
32397299	3	10	theme	scanning	504:511	arg1	SEM					534:536	SEM	534:536	SEM	534:536	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	3	10	theme	scanning	504:511	arg1	microscopy					522:531	scanning electron microscopy	504:531	scanning electron microscopy (SEM)	504:537	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	4	11	theme	biochemical	620:630	arg1	activities					632:641	biochemical activities	620:641	biochemical activities	620:641	The functional properties and biochemical activities of EAP were determined.					
32397299	9	12	theme	solubility	1209:1218	arg1	properties					1197:1206	interesting functional properties	1174:1206	interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties)	1174:1274	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	9	12	theme	solubility	1209:1218	arg1	properties					1264:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	6	13	theme	extracted	857:865	arg1	EAP					867:869	the extracted EAP	853:869	the extracted EAP	853:869	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	7	14	theme	porosity	984:991	arg1	particles					993:1001	numerous high porosity particles	970:1001	numerous high porosity particles	970:1001	SEM images showed that EAP present numerous high porosity particles.					
32397299	3	15	theme	electron	513:520	arg1	SEM					534:536	SEM	534:536	SEM	534:536	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	3	15	theme	electron	513:520	arg1	microscopy					522:531	scanning electron microscopy	504:531	scanning electron microscopy (SEM)	504:537	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	5	16	theme	±	787:787	arg1	%					793:793	6.82% ± 0.57%	781:793	6.82% ± 0.57%	781:793	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	10	17	theme	antioxidant	1357:1367	arg1	activities					1369:1378	excellent antihypertensive and antioxidant activities	1326:1378	excellent antihypertensive and antioxidant activities	1326:1378	Finally, the results revealed that EAP displayed excellent antihypertensive and antioxidant activities.					
32397299	6	18	dep	groups	908:913	arg1	C-H					921:923	C-H	921:923	C-H	921:923	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	6	18	dep	groups	908:913	arg1	O-H					930:932	O-H	930:932	O-H	930:932	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	6	18	dep	groups	908:913	arg1	C=O					916:918	C=O	916:918	C=O	916:918	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	6	18	dep	groups	908:913	arg1	groups					908:913	three functional groups	891:913	three functional groups: C=O, C-H, and O-H	891:932	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	11	19	theme	antioxidants	1448:1459	arg1	source					1422:1427	a promising natural source	1402:1427	a promising natural source of food additives, antioxidants, and antihypertensive agents	1402:1488	Overall, EAP present a promising natural source of food additives, antioxidants, and antihypertensive agents.					
32397299	3	20	dep	Fourier	455:461	arg1	transform					463:471	transform	463:471	transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS)	463:587	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	2	21	theme	water	383:387	arg1	bath					389:392	a water bath	381:392	a water bath at 90 °C	381:401	EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C.					
32397299	0	22	theme	Antioxidant	112:122	arg1	Polysaccharides					14:28	Water-Soluble Polysaccharides	0:28	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.	0:132	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	0	22	theme	Antioxidant	112:122	arg1	Activity					124:131	Antioxidant Activity	112:131	Antioxidant Activity	112:131	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	0	23	from	Stems	49:53	arg1	Characterization					67:82	Structural Characterization	56:82	Structural Characterization	56:82	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	0	23	from	Stems	49:53	arg1	Polysaccharides					14:28	Water-Soluble Polysaccharides	0:28	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.	0:132	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	0	23	from	Stems	49:53	arg1	Activity					124:131	Antioxidant Activity	112:131	Antioxidant Activity	112:131	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	7	24	theme	numerous	970:977	arg1	particles					993:1001	numerous high porosity particles	970:1001	numerous high porosity particles	970:1001	SEM images showed that EAP present numerous high porosity particles.					
32397299	3	25	dep	transform	463:471	arg1	infrared					473:480	infrared	473:480	transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS)	463:587	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	10	26	theme	antihypertensive	1336:1351	arg1	activities					1369:1378	excellent antihypertensive and antioxidant activities	1326:1378	excellent antihypertensive and antioxidant activities	1326:1378	Finally, the results revealed that EAP displayed excellent antihypertensive and antioxidant activities.					
32397299	1	27	theme	functional	187:196	arg1	properties					198:207	functional properties	187:207	functional properties	187:207	In this study, the physicochemical characterization, functional properties, and antioxidant activity of polysaccharides extracted from Ephedra alata (EAP) were investigated.					
32397299	5	28	theme	acid	741:744	arg1	contents					707:714	the contents	703:714	the contents of carbohydrates, uronic acid, and proteins	703:758	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	5	28	theme	acid	741:744	arg1	%					778:778	73.24% ± 1.24%	765:778	73.24% ± 1.24%	765:778	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	8	29	theme	monosaccharide	1008:1021	arg1	composition					1023:1033	The monosaccharide composition	1004:1033	The monosaccharide composition	1004:1033	The monosaccharide composition revealed a polymer composed of glucose (43.1%), galactose (36.4%), mannose (14.9%), arabinose (3.7%), and gluconic acid (1.7%).					
32397299	11	30	theme	antihypertensive	1466:1481	arg1	agents					1483:1488	antihypertensive agents	1466:1488	antihypertensive agents	1466:1488	Overall, EAP present a promising natural source of food additives, antioxidants, and antihypertensive agents.					
32397299	9	31	theme	capacity	1233:1240	arg1	properties					1197:1206	interesting functional properties	1174:1206	interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties)	1174:1274	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	9	31	theme	capacity	1233:1240	arg1	properties					1264:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	0	32	theme	Water-Soluble	0:12	arg1	Characterization					67:82	Structural Characterization	56:82	Structural Characterization	56:82	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	0	32	theme	Water-Soluble	0:12	arg1	Polysaccharides					14:28	Water-Soluble Polysaccharides	0:28	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.	0:132	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	0	32	theme	Water-Soluble	0:12	arg1	Activity					124:131	Antioxidant Activity	112:131	Antioxidant Activity	112:131	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	6	33	contain	contain	883:889	arg2	C-H					921:923	C-H	921:923	C-H	921:923	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	6	33	contain	contain	883:889	arg2	C=O					916:918	C=O	916:918	C=O	916:918	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	6	33	contain	contain	883:889	arg2	O-H					930:932	O-H	930:932	O-H	930:932	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	6	33	contain	contain	883:889	arg2	groups					908:913	three functional groups	891:913	three functional groups: C=O, C-H, and O-H	891:932	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	6	33	contain	contain	883:889	arg1	EAP					867:869	the extracted EAP	853:869	the extracted EAP	853:869	The results showed that the extracted EAP essentially contain three functional groups: C=O, C-H, and O-H.					
32397299	2	34	theme	5	376:376	arg1	ratio					367:371	a liquid/solid ratio	352:371	a liquid/solid ratio of 5 in a water bath at 90 °C	352:401	EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C.					
32397299	0	35	theme	Ephedra	35:41	arg1	Stems					49:53	Ephedra alata Stems	35:53	Ephedra alata Stems	35:53	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	5	36	theme	%	770:770	arg1	contents					707:714	the contents	703:714	the contents of carbohydrates, uronic acid, and proteins	703:758	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	5	36	theme	%	770:770	arg1	%					778:778	73.24% ± 1.24%	765:778	73.24% ± 1.24%	765:778	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	2	37	from	ratio	367:371	arg1	bath					389:392	a water bath	381:392	a water bath at 90 °C	381:401	EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C.					
32397299	2	37	from	ratio	367:371	arg1	°C					400:401	90 °C	397:401	90 °C	397:401	EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C.					
32397299	4	38	dep	properties	605:614	arg1	The					590:592	The	590:592	The	590:592	The functional properties and biochemical activities of EAP were determined.					
32397299	3	39	theme	EAP	435:437	arg1	structure					408:416	The structure	404:416	The structure of the extracted EAP	404:437	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	5	40	theme	chemical	671:678	arg1	analysis					680:687	The chemical analysis	667:687	The chemical analysis	667:687	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	5	41	theme	±	772:772	arg1	contents					707:714	the contents	703:714	the contents of carbohydrates, uronic acid, and proteins	703:758	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	5	41	theme	±	772:772	arg1	%					778:778	73.24% ± 1.24%	765:778	73.24% ± 1.24%	765:778	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	1	42	theme	antioxidant	214:224	arg1	activity					226:233	antioxidant activity	214:233	antioxidant activity	214:233	In this study, the physicochemical characterization, functional properties, and antioxidant activity of polysaccharides extracted from Ephedra alata (EAP) were investigated.					
32397299	3	43	theme	gas	544:546	arg1	GC-MS					582:586	GC-MS	582:586	GC-MS	582:586	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	3	43	theme	gas	544:546	arg1	spectrometry					568:579	gas chromatography-mass spectrometry	544:579	gas chromatography-mass spectrometry (GC-MS)	544:587	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	9	44	theme	emulsion	1255:1262	arg1	properties					1197:1206	interesting functional properties	1174:1206	interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties)	1174:1274	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	9	44	theme	emulsion	1255:1262	arg1	properties					1264:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	0	45	theme	alata	43:47	arg1	Stems					49:53	Ephedra alata Stems	35:53	Ephedra alata Stems	35:53	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	7	46	theme	SEM	935:937	arg1	images					939:944	SEM images	935:944	SEM images	935:944	SEM images showed that EAP present numerous high porosity particles.					
32397299	11	47	theme	agents	1483:1488	arg1	source					1422:1427	a promising natural source	1402:1427	a promising natural source of food additives, antioxidants, and antihypertensive agents	1402:1488	Overall, EAP present a promising natural source of food additives, antioxidants, and antihypertensive agents.					
32397299	3	48	theme	chromatography-mass	548:566	arg1	GC-MS					582:586	GC-MS	582:586	GC-MS	582:586	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	3	48	theme	chromatography-mass	548:566	arg1	spectrometry					568:579	gas chromatography-mass spectrometry	544:579	gas chromatography-mass spectrometry (GC-MS)	544:587	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	5	49	theme	%	785:785	arg1	%					793:793	6.82% ± 0.57%	781:793	6.82% ± 0.57%	781:793	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	3	50	theme	extracted	425:433	arg1	EAP					435:437	the extracted EAP	421:437	the extracted EAP	421:437	The structure of the extracted EAP was examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and gas chromatography-mass spectrometry (GC-MS).					
32397299	0	51	theme	Structural	56:65	arg1	Characterization					67:82	Structural Characterization	56:82	Structural Characterization	56:82	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	0	51	theme	Structural	56:65	arg1	Polysaccharides					14:28	Water-Soluble Polysaccharides	0:28	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.	0:132	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	2	52	with	h	345:345	arg1	ratio					367:371	a liquid/solid ratio	352:371	a liquid/solid ratio of 5 in a water bath at 90 °C	352:401	EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C.					
32397299	9	53	theme	oil	1221:1223	arg1	capacity					1233:1240	oil holding capacity	1221:1240	oil holding capacity	1221:1240	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	11	54	theme	additives	1437:1445	arg1	source					1422:1427	a promising natural source	1402:1427	a promising natural source of food additives, antioxidants, and antihypertensive agents	1402:1488	Overall, EAP present a promising natural source of food additives, antioxidants, and antihypertensive agents.					
32397299	1	55	theme	polysaccharides	238:252	arg1	properties					198:207	functional properties	187:207	functional properties	187:207	In this study, the physicochemical characterization, functional properties, and antioxidant activity of polysaccharides extracted from Ephedra alata (EAP) were investigated.					
32397299	1	55	theme	polysaccharides	238:252	arg1	activity					226:233	antioxidant activity	214:233	antioxidant activity	214:233	In this study, the physicochemical characterization, functional properties, and antioxidant activity of polysaccharides extracted from Ephedra alata (EAP) were investigated.					
32397299	1	55	theme	polysaccharides	238:252	arg1	characterization					169:184	the physicochemical characterization	149:184	the physicochemical characterization	149:184	In this study, the physicochemical characterization, functional properties, and antioxidant activity of polysaccharides extracted from Ephedra alata (EAP) were investigated.					
32397299	8	56	theme	gluconic	1141:1148	arg1	acid					1150:1153	gluconic acid	1141:1153	gluconic acid (1.7%)	1141:1160	The monosaccharide composition revealed a polymer composed of glucose (43.1%), galactose (36.4%), mannose (14.9%), arabinose (3.7%), and gluconic acid (1.7%).					
32397299	8	56	theme	gluconic	1141:1148	arg1	%					1159:1159	1.7%	1156:1159	1.7%	1156:1159	The monosaccharide composition revealed a polymer composed of glucose (43.1%), galactose (36.4%), mannose (14.9%), arabinose (3.7%), and gluconic acid (1.7%).					
32397299	11	57	theme	natural	1414:1420	arg1	source					1422:1427	a promising natural source	1402:1427	a promising natural source of food additives, antioxidants, and antihypertensive agents	1402:1488	Overall, EAP present a promising natural source of food additives, antioxidants, and antihypertensive agents.					
32397299	5	58	theme	carbohydrates	719:731	arg1	contents					707:714	the contents	703:714	the contents of carbohydrates, uronic acid, and proteins	703:758	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	5	58	theme	carbohydrates	719:731	arg1	%					778:778	73.24% ± 1.24%	765:778	73.24% ± 1.24%	765:778	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	4	59	theme	functional	594:603	arg1	properties					605:614	functional properties	594:614	functional properties	594:614	The functional properties and biochemical activities of EAP were determined.					
32397299	0	60	dep	Polysaccharides	14:28	arg1	Characterization					67:82	Structural Characterization	56:82	Structural Characterization	56:82	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	0	60	dep	Polysaccharides	14:28	arg1	Properties					96:105	Functional Properties	85:105	Functional Properties	85:105	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	0	60	dep	Polysaccharides	14:28	arg1	Polysaccharides					14:28	Water-Soluble Polysaccharides	0:28	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.	0:132	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	0	60	dep	Polysaccharides	14:28	arg1	Activity					124:131	Antioxidant Activity	112:131	Antioxidant Activity	112:131	Water-Soluble Polysaccharides from Ephedra alata Stems: Structural Characterization, Functional Properties, and Antioxidant Activity.					
32397299	9	61	theme	foaming	1243:1249	arg1	properties					1197:1206	interesting functional properties	1174:1206	interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties)	1174:1274	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	9	61	theme	foaming	1243:1249	arg1	properties					1264:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	9	62	theme	holding	1225:1231	arg1	capacity					1233:1240	oil holding capacity	1221:1240	oil holding capacity	1221:1240	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	9	63	theme	interesting	1174:1184	arg1	properties					1197:1206	interesting functional properties	1174:1206	interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties)	1174:1274	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	9	63	theme	interesting	1174:1184	arg1	properties					1264:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	5	64	theme	proteins	751:758	arg1	contents					707:714	the contents	703:714	the contents of carbohydrates, uronic acid, and proteins	703:758	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	5	64	theme	proteins	751:758	arg1	%					778:778	73.24% ± 1.24%	765:778	73.24% ± 1.24%	765:778	The chemical analysis revealed that the contents of carbohydrates, uronic acid, and proteins were 73.24% ± 1.24%, 6.82% ± 0.57%, and 6.56% ± 0.36%, respectively.					
32397299	1	65	theme	physicochemical	153:167	arg1	characterization					169:184	the physicochemical characterization	149:184	the physicochemical characterization	149:184	In this study, the physicochemical characterization, functional properties, and antioxidant activity of polysaccharides extracted from Ephedra alata (EAP) were investigated.					
32397299	2	66	from	°C	400:401	arg1	ratio					367:371	a liquid/solid ratio	352:371	a liquid/solid ratio of 5 in a water bath at 90 °C	352:401	EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C.					
32397299	2	66	from	°C	400:401	arg1	bath					389:392	a water bath	381:392	a water bath at 90 °C	381:401	EAP were extracted in water during 3 h with a liquid/solid ratio of 5 in a water bath at 90 °C.					
32397299	4	67	theme	EAP	646:648	arg1	properties					605:614	functional properties	594:614	functional properties	594:614	The functional properties and biochemical activities of EAP were determined.					
32397299	4	67	theme	EAP	646:648	arg1	activities					632:641	biochemical activities	620:641	biochemical activities	620:641	The functional properties and biochemical activities of EAP were determined.					
32397299	9	68	theme	functional	1186:1195	arg1	properties					1197:1206	interesting functional properties	1174:1206	interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties)	1174:1274	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
32397299	9	68	theme	functional	1186:1195	arg1	properties					1264:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	solubility, oil holding capacity, foaming and emulsion properties	1209:1273	EAP showed interesting functional properties (solubility, oil holding capacity, foaming and emulsion properties).					
33838798	1	0	theme	biomedical	325:334	arg1	technologies					336:347	biomedical technologies	325:347	biomedical technologies	325:347	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	5	1	from	self-organization	794:810	arg1	surface					846:852	the surface	842:852	the surface of chitin nanofibrils	842:874	These results are related to the appearance of oriented structure in nanocomposites and to self-organization of chitosan macromolecules on the surface of chitin nanofibrils.					
33838798	4	2	from	fibroblasts	674:684	arg1	surface					694:700	film surface	689:700	film surface	689:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	3	theme	enhanced	555:562	arg1	properties					575:584	enhanced mechanical properties	555:584	enhanced mechanical properties	555:584	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	5	4	theme	oriented	750:757	arg1	structure					759:767	oriented structure	750:767	oriented structure	750:767	These results are related to the appearance of oriented structure in nanocomposites and to self-organization of chitosan macromolecules on the surface of chitin nanofibrils.					
33838798	1	5	from	use	318:320	arg1	technologies					336:347	biomedical technologies	325:347	biomedical technologies	325:347	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	6	from	study	141:145	arg1	conductivity					258:269	electrical conductivity	247:269	electrical conductivity	247:269	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	6	from	study	141:145	arg1	structure					189:197	structure	189:197	structure	189:197	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	6	from	study	141:145	arg1	morphology					208:217	surface morphology	200:217	surface morphology	200:217	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	6	from	study	141:145	arg1	properties					231:240	mechanical properties	220:240	mechanical properties	220:240	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	4	7	theme	fibroblasts	674:684	arg1	conductivity					626:637	specific conductivity	617:637	specific conductivity	617:637	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	7	theme	fibroblasts	674:684	arg1	activity					657:664	proliferative activity	643:664	proliferative activity	643:664	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	0	8	theme	biological	90:99	arg1	properties					101:110	their biological properties	84:110	their biological properties	84:110	Influence of surface morphology of chitosan films modified by chitin nanofibrils on their biological properties.					
33838798	6	9	theme	surface	929:935	arg1	hydrophilicity					937:950	surface energy and surface hydrophilicity	910:950	hydrophilicity	937:950	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	4	10	from	activity	657:664	arg1	surface					694:700	film surface	689:700	film surface	689:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	1	11	theme	electrical	247:256	arg1	conductivity					258:269	electrical conductivity	247:269	electrical conductivity	247:269	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	4	12	theme	specific	617:624	arg1	conductivity					626:637	specific conductivity	617:637	specific conductivity	617:637	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	2	13	theme	optimal	379:385	arg1	concentration					387:399	the optimal concentration	375:399	the optimal concentration of chitin nanofibrils in the composite film	375:443	It was demonstrated that the optimal concentration of chitin nanofibrils in the composite film is 5 wt.					
33838798	2	13	theme	optimal	379:385	arg1	wt					450:451	5 wt	448:451	5 wt	448:451	It was demonstrated that the optimal concentration of chitin nanofibrils in the composite film is 5 wt.					
33838798	1	14	theme	influence	150:158	arg1	study					141:145	the study	137:145	the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies	137:347	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	4	15	theme	mechanical	564:573	arg1	properties					575:584	enhanced mechanical properties	555:584	enhanced mechanical properties	555:584	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	5	16	theme	structure	759:767	arg1	appearance					736:745	the appearance	732:745	the appearance of oriented structure in nanocomposites	732:785	These results are related to the appearance of oriented structure in nanocomposites and to self-organization of chitosan macromolecules on the surface of chitin nanofibrils.					
33838798	5	17	theme	chitin	857:862	arg1	nanofibrils					864:874	chitin nanofibrils	857:874	chitin nanofibrils	857:874	These results are related to the appearance of oriented structure in nanocomposites and to self-organization of chitosan macromolecules on the surface of chitin nanofibrils.					
33838798	4	18	from	surface	546:552	arg1	orientation					503:513	orientation	503:513	orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface	503:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	18	from	surface	546:552	arg1	conductivity					626:637	specific conductivity	617:637	specific conductivity	617:637	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	18	from	surface	546:552	arg1	activity					657:664	proliferative activity	643:664	proliferative activity	643:664	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	19	theme	film	541:544	arg1	surface					546:552	film surface	541:552	film surface	541:552	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	1	20	from	conductivity	258:269	arg1	study					141:145	the study	137:145	the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies	137:347	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	4	21	from	conductivity	626:637	arg1	surface					694:700	film surface	689:700	film surface	689:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	1	22	theme	chitin	163:168	arg1	nanofibrils					170:180	chitin nanofibrils	163:180	chitin nanofibrils	163:180	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	6	23	theme	proliferative	1005:1017	arg1	activity					1019:1026	proliferative activity	1005:1026	proliferative activity	1005:1026	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	1	24	theme	nanofibrils	170:180	arg1	influence					150:158	influence	150:158	influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies	150:347	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	0	25	theme	morphology	21:30	arg1	Influence					0:8	Influence	0:8	Influence of surface morphology of chitosan films	0:48	Influence of surface morphology of chitosan films modified by chitin nanofibrils on their biological properties.					
33838798	4	26	theme	elements	529:536	arg1	orientation					503:513	orientation	503:513	orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface	503:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	5	27	theme	macromolecules	824:837	arg1	self-organization					794:810	self-organization	794:810	self-organization of chitosan macromolecules on the surface of chitin nanofibrils	794:874	These results are related to the appearance of oriented structure in nanocomposites and to self-organization of chitosan macromolecules on the surface of chitin nanofibrils.					
33838798	0	28	theme	surface	13:19	arg1	morphology					21:30	surface morphology	13:30	surface morphology of chitosan films	13:48	Influence of surface morphology of chitosan films modified by chitin nanofibrils on their biological properties.					
33838798	1	29	theme	chitosan-based	274:287	arg1	films					299:303	chitosan-based composite films	274:303	chitosan-based composite films intended for use in biomedical technologies	274:347	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	4	30	from	increase	600:607	arg1	orientation					503:513	orientation	503:513	orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface	503:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	30	from	increase	600:607	arg1	conductivity					626:637	specific conductivity	617:637	specific conductivity	617:637	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	30	from	increase	600:607	arg1	activity					657:664	proliferative activity	643:664	proliferative activity	643:664	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	6	31	theme	surface	910:916	arg1	energy					918:923	surface energy and surface hydrophilicity	910:950	energy	918:923	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	0	32	theme	chitosan	35:42	arg1	films					44:48	chitosan films	35:48	chitosan films	35:48	Influence of surface morphology of chitosan films modified by chitin nanofibrils on their biological properties.					
33838798	1	33	theme	composite	289:297	arg1	films					299:303	chitosan-based composite films	274:303	chitosan-based composite films intended for use in biomedical technologies	274:347	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	34	from	properties	231:240	arg1	study					141:145	the study	137:145	the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies	137:347	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	0	35	from	nanofibrils	69:79	arg1	properties					101:110	their biological properties	84:110	their biological properties	84:110	Influence of surface morphology of chitosan films modified by chitin nanofibrils on their biological properties.					
33838798	4	36	theme	proliferative	643:655	arg1	activity					657:664	proliferative activity	643:664	proliferative activity	643:664	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	2	37	theme	nanofibrils	411:421	arg1	concentration					387:399	the optimal concentration	375:399	the optimal concentration of chitin nanofibrils in the composite film	375:443	It was demonstrated that the optimal concentration of chitin nanofibrils in the composite film is 5 wt.					
33838798	2	37	theme	nanofibrils	411:421	arg1	wt					450:451	5 wt	448:451	5 wt	448:451	It was demonstrated that the optimal concentration of chitin nanofibrils in the composite film is 5 wt.					
33838798	6	38	theme	composite	1074:1082	arg1	films					1084:1088	composite films	1074:1088	composite films	1074:1088	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	1	39	theme	films	299:303	arg1	conductivity					258:269	electrical conductivity	247:269	electrical conductivity	247:269	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	39	theme	films	299:303	arg1	structure					189:197	structure	189:197	structure	189:197	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	39	theme	films	299:303	arg1	morphology					208:217	surface morphology	200:217	surface morphology	200:217	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	39	theme	films	299:303	arg1	properties					231:240	mechanical properties	220:240	mechanical properties	220:240	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	5	40	theme	nanofibrils	864:874	arg1	surface					846:852	the surface	842:852	the surface of chitin nanofibrils	842:874	These results are related to the appearance of oriented structure in nanocomposites and to self-organization of chitosan macromolecules on the surface of chitin nanofibrils.					
33838798	1	41	from	structure	189:197	arg1	study					141:145	the study	137:145	the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies	137:347	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	2	42	theme	chitin	404:409	arg1	nanofibrils					411:421	chitin nanofibrils	404:421	chitin nanofibrils	404:421	It was demonstrated that the optimal concentration of chitin nanofibrils in the composite film is 5 wt.					
33838798	4	43	from	orientation	503:513	arg1	increase					600:607	an increase	597:607	an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface	597:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	43	from	orientation	503:513	arg1	properties					575:584	enhanced mechanical properties	555:584	enhanced mechanical properties	555:584	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	43	from	orientation	503:513	arg1	surface					546:552	film surface	541:552	film surface	541:552	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	44	from	properties	575:584	arg1	orientation					503:513	orientation	503:513	orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface	503:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	44	from	properties	575:584	arg1	conductivity					626:637	specific conductivity	617:637	specific conductivity	617:637	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	44	from	properties	575:584	arg1	activity					657:664	proliferative activity	643:664	proliferative activity	643:664	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	5	45	theme	chitosan	815:822	arg1	macromolecules					824:837	chitosan macromolecules	815:837	chitosan macromolecules	815:837	These results are related to the appearance of oriented structure in nanocomposites and to self-organization of chitosan macromolecules on the surface of chitin nanofibrils.					
33838798	6	46	theme	effective	971:979	arg1	adhesion					981:988	effective adhesion	971:988	effective adhesion	971:988	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	0	47	theme	films	44:48	arg1	morphology					21:30	surface morphology	13:30	surface morphology of chitosan films	13:48	Influence of surface morphology of chitosan films modified by chitin nanofibrils on their biological properties.					
33838798	4	48	theme	structural	518:527	arg1	elements					529:536	structural elements	518:536	structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface	518:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	49	theme	film	689:692	arg1	surface					694:700	film surface	689:700	film surface	689:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	1	50	dep	structure	189:197	arg1	the					185:187	the	185:187	the	185:187	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	2	51	from	concentration	387:399	arg1	film					440:443	the composite film	426:443	the composite film	426:443	It was demonstrated that the optimal concentration of chitin nanofibrils in the composite film is 5 wt.					
33838798	1	52	theme	surface	200:206	arg1	morphology					208:217	surface morphology	200:217	surface morphology	200:217	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	53	from	morphology	208:217	arg1	study					141:145	the study	137:145	the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies	137:347	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	4	54	from	elements	529:536	arg1	increase					600:607	an increase	597:607	an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface	597:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	54	from	elements	529:536	arg1	properties					575:584	enhanced mechanical properties	555:584	enhanced mechanical properties	555:584	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	54	from	elements	529:536	arg1	surface					546:552	film surface	541:552	film surface	541:552	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	55	from	surface	694:700	arg1	conductivity					626:637	specific conductivity	617:637	specific conductivity	617:637	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	4	55	from	surface	694:700	arg1	activity					657:664	proliferative activity	643:664	proliferative activity	643:664	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	6	56	theme	cells	1031:1035	arg1	viability					991:999	viability	991:999	viability	991:999	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	6	56	theme	cells	1031:1035	arg1	activity					1019:1026	proliferative activity	1005:1026	proliferative activity	1005:1026	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	6	56	theme	cells	1031:1035	arg1	adhesion					981:988	effective adhesion	971:988	effective adhesion	971:988	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	0	57	theme	chitin	62:67	arg1	nanofibrils					69:79	chitin nanofibrils	62:79	chitin nanofibrils on their biological properties	62:110	Influence of surface morphology of chitosan films modified by chitin nanofibrils on their biological properties.					
33838798	5	58	from	appearance	736:745	arg1	nanocomposites					772:785	nanocomposites	772:785	nanocomposites	772:785	These results are related to the appearance of oriented structure in nanocomposites and to self-organization of chitosan macromolecules on the surface of chitin nanofibrils.					
33838798	4	59	theme	skin	669:672	arg1	fibroblasts					674:684	skin fibroblasts	669:684	skin fibroblasts on film surface	669:700	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	6	60	theme	films	1084:1088	arg1	surface					1063:1069	the surface	1059:1069	the surface of composite films	1059:1088	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	6	61	from	increase	898:905	arg1	hydrophilicity					937:950	surface energy and surface hydrophilicity	910:950	hydrophilicity	937:950	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	6	61	from	increase	898:905	arg1	energy					918:923	surface energy and surface hydrophilicity	910:950	energy	918:923	It was revealed that increase in surface energy and surface hydrophilicity did not facilitate effective adhesion, viability and proliferative activity of cells during cultivation on the surface of composite films.					
33838798	4	62	theme	composition	478:488	arg1	films					464:468	the films	460:468	the films of this composition	460:488	For the films of this composition, we observed orientation of structural elements on film surface, enhanced mechanical properties as well as an increase in both specific conductivity and proliferative activity of skin fibroblasts on film surface.					
33838798	2	63	theme	composite	430:438	arg1	film					440:443	the composite film	426:443	the composite film	426:443	It was demonstrated that the optimal concentration of chitin nanofibrils in the composite film is 5 wt.					
33838798	1	64	from	influence	150:158	arg1	conductivity					258:269	electrical conductivity	247:269	electrical conductivity	247:269	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	64	from	influence	150:158	arg1	structure					189:197	structure	189:197	structure	189:197	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	64	from	influence	150:158	arg1	morphology					208:217	surface morphology	200:217	surface morphology	200:217	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	64	from	influence	150:158	arg1	properties					231:240	mechanical properties	220:240	mechanical properties	220:240	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838798	1	65	theme	mechanical	220:229	arg1	properties					231:240	mechanical properties	220:240	mechanical properties	220:240	The paper is devoted to the study of influence of chitin nanofibrils on the structure, surface morphology, mechanical properties, and electrical conductivity of chitosan-based composite films intended for use in biomedical technologies.					
33838818	4	0	theme	cells	1030:1034	arg1	types					1014:1018	different types	1004:1018	different types	1004:1018	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	1	theme	S	735:735	arg1	S-Hb					782:785	S-Hb	782:785	S-Hb	782:785	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	1	theme	S	735:735	arg1	[d-QuipNAc4N					769:780	2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6	704:867	[d-QuipNAc4N	769:780	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	1	theme	S	735:735	arg1	S-Pyr					869:873	S-Pyr	869:873	S-Pyr	869:873	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	2	2	from	effect	405:410	arg1	225T					392:395	P. submarinus KMM 225T	374:395	P. submarinus KMM 225T	374:395	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	2	2	from	effect	405:410	arg1	viability					419:427	viability	419:427	viability	419:427	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	2	2	from	effect	405:410	arg1	formation					440:448	colony formation	433:448	colony formation	433:448	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	1	3	theme	KMM	176:178	arg1	bacterium					204:212	a Gram-negative bacterium	188:212	a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean	188:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	1	3	theme	KMM	176:178	arg1	225T					180:183	Psychrobacter submarinus KMM 225T	151:183	Psychrobacter submarinus KMM 225T	151:183	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	0	4	theme	polysaccharide	73:86	arg1	activity					41:48	in vitro antiproliferative activity	14:48	in vitro antiproliferative activity	14:48	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	0	4	theme	polysaccharide	73:86	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	4	5	contain	containing	693:702	arg2	S-Pyr					869:873	S-Pyr	869:873	S-Pyr	869:873	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	5	contain	containing	693:702	arg2	[d-QuipNAc4N					769:780	2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6	704:867	[d-QuipNAc4N	769:780	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	5	contain	containing	693:702	arg2	S-Hb					782:785	S-Hb	782:785	S-Hb	782:785	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	5	contain	containing	693:702	arg2	S					801:801	S	801:801	S	801:801	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	5	contain	containing	693:702	arg2	[d-GlcpNAc4,6					855:867	2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6	704:867	[d-GlcpNAc4,6	855:867	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	5	contain	containing	693:702	arg1	polysaccharide					677:690	the acidic capsular polysaccharide	657:690	the acidic capsular polysaccharide	657:690	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	6	theme	human	1095:1099	arg1	cells					1136:1140	the human chronic myelogenous leukemia K-562 cells	1091:1140	the human chronic myelogenous leukemia K-562 cells	1091:1140	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	0	7	theme	deep-sea	97:104	arg1	bacterium					106:114	the deep-sea bacterium	93:114	the deep-sea bacterium	93:114	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	0	8	from	Structure	0:8	arg1	bacterium					106:114	the deep-sea bacterium	93:114	the deep-sea bacterium	93:114	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	4	9	theme	colony	984:989	arg1	formation					991:999	the colony formation	980:999	the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells	980:1140	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	10	theme	following	634:642	arg1	structure					644:652	The following structure	630:652	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)]	630:875	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	11	theme	capsular	898:905	arg1	polysaccharide					907:920	The capsular polysaccharide	894:920	The capsular polysaccharide	894:920	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	2	12	theme	capsular	345:352	arg1	polysaccharide					354:367	the capsular polysaccharide	341:367	the capsular polysaccharide from P. submarinus KMM 225T	341:395	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	1	13	theme	m	275:275	arg1	depth					262:266	a depth	260:266	a depth of 300 m in the Northwest Pacific Ocean	260:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	4	14	theme	different	1004:1012	arg1	types					1014:1018	different types	1004:1018	different types	1004:1018	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	2	15	dep	viability	419:427	arg1	the					415:417	the	415:417	the	415:417	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	2	16	from	structure	328:336	arg1	225T					392:395	P. submarinus KMM 225T	374:395	P. submarinus KMM 225T	374:395	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	2	16	from	structure	328:336	arg1	viability					419:427	viability	419:427	viability	419:427	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	2	16	from	structure	328:336	arg1	formation					440:448	colony formation	433:448	colony formation	433:448	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	3	17	theme	chromatography	524:537	arg1	methods					539:545	chromatography methods	524:545	chromatography methods	524:545	The glycopolymer was purified by ultracentrifugation and chromatography methods, and the structure was elucidated using NMR spectroscopy and composition analyses.					
33838818	2	18	theme	submarinus	377:386	arg1	225T					392:395	P. submarinus KMM 225T	374:395	P. submarinus KMM 225T	374:395	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	4	19	theme	capsular	668:675	arg1	polysaccharide					677:690	the acidic capsular polysaccharide	657:690	the acidic capsular polysaccharide	657:690	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	0	20	from	activity	41:48	arg1	bacterium					106:114	the deep-sea bacterium	93:114	the deep-sea bacterium	93:114	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	4	21	theme	pronounced	1055:1064	arg1	inhibition					1066:1075	the most pronounced inhibition	1046:1075	the most pronounced inhibition	1046:1075	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	22	theme	4,6-O-[	793:799	arg1	[d-GlcpNAc4,6					855:867	2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6	704:867	[d-GlcpNAc4,6	855:867	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	22	theme	4,6-O-[	793:799	arg1	S					801:801	S	801:801	S	801:801	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	2	23	theme	KMM	388:390	arg1	225T					392:395	P. submarinus KMM 225T	374:395	P. submarinus KMM 225T	374:395	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	2	24	theme	colony	433:438	arg1	formation					440:448	colony formation	433:448	colony formation	433:448	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	4	25	theme	cancer	1023:1028	arg1	cells					1030:1034	cancer cells	1023:1034	cancer cells	1023:1034	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	26	theme	acidic	661:666	arg1	polysaccharide					677:690	the acidic capsular polysaccharide	657:690	the acidic capsular polysaccharide	657:690	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	1	27	theme	Gram-negative	190:202	arg1	bacterium					204:212	a Gram-negative bacterium	188:212	a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean	188:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	1	27	theme	Gram-negative	190:202	arg1	225T					180:183	Psychrobacter submarinus KMM 225T	151:183	Psychrobacter submarinus KMM 225T	151:183	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	2	28	theme	P.	374:375	arg1	225T					392:395	P. submarinus KMM 225T	374:395	P. submarinus KMM 225T	374:395	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	3	29	theme	NMR	587:589	arg1	spectroscopy					591:602	NMR spectroscopy	587:602	NMR spectroscopy	587:602	The glycopolymer was purified by ultracentrifugation and chromatography methods, and the structure was elucidated using NMR spectroscopy and composition analyses.					
33838818	0	30	theme	in	14:15	arg1	activity					41:48	in vitro antiproliferative activity	14:48	in vitro antiproliferative activity	14:48	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	1	31	theme	Northwest	284:292	arg1	Ocean					302:306	the Northwest Pacific Ocean	280:306	the Northwest Pacific Ocean	280:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	4	32	theme	2-acetamido-2,4,6-trideoxy-4-[	704:733	arg1	S-Hb					782:785	S-Hb	782:785	S-Hb	782:785	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	32	theme	2-acetamido-2,4,6-trideoxy-4-[	704:733	arg1	[d-QuipNAc4N					769:780	2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6	704:867	[d-QuipNAc4N	769:780	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	32	theme	2-acetamido-2,4,6-trideoxy-4-[	704:733	arg1	S-Pyr					869:873	S-Pyr	869:873	S-Pyr	869:873	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	2	33	theme	cells	460:464	arg1	viability					419:427	viability	419:427	viability	419:427	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	2	33	theme	cells	460:464	arg1	formation					440:448	colony formation	433:448	colony formation	433:448	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	1	34	theme	Pacific	294:300	arg1	Ocean					302:306	the Northwest Pacific Ocean	280:306	the Northwest Pacific Ocean	280:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	1	35	from	depth	262:266	arg1	Ocean					302:306	the Northwest Pacific Ocean	280:306	the Northwest Pacific Ocean	280:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	4	36	theme	polysaccharide	677:690	arg1	structure					644:652	The following structure	630:652	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)]	630:875	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	0	37	theme	antiproliferative	23:39	arg1	activity					41:48	in vitro antiproliferative activity	14:48	in vitro antiproliferative activity	14:48	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	2	38	theme	cancer	453:458	arg1	cells					460:464	cancer cells	453:464	cancer cells	453:464	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	4	39	theme	-2-acetamido-2-deoxy-d-glucose	824:853	arg1	[d-GlcpNAc4,6					855:867	2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6	704:867	[d-GlcpNAc4,6	855:867	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	39	theme	-2-acetamido-2-deoxy-d-glucose	824:853	arg1	S					801:801	S	801:801	S	801:801	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	1	40	theme	sea-water	230:238	arg1	sample					240:245	a sea-water sample	228:245	a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean	228:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	4	41	theme	types	1014:1018	arg1	formation					991:999	the colony formation	980:999	the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells	980:1140	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	42	theme	-3-hydroxybutyramido	737:756	arg1	S-Hb					782:785	S-Hb	782:785	S-Hb	782:785	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	42	theme	-3-hydroxybutyramido	737:756	arg1	[d-QuipNAc4N					769:780	2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6	704:867	[d-QuipNAc4N	769:780	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	42	theme	-3-hydroxybutyramido	737:756	arg1	S-Pyr					869:873	S-Pyr	869:873	S-Pyr	869:873	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	2	43	theme	polysaccharide	354:367	arg1	structure					328:336	the structure	324:336	the structure of the capsular polysaccharide from P. submarinus KMM 225T	324:395	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	2	43	theme	polysaccharide	354:367	arg1	effect					405:410	its effect	401:410	its effect on the viability and colony formation of cancer cells	401:464	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	1	44	attach	isolated	214:221	arg2	225T					180:183	Psychrobacter submarinus KMM 225T	151:183	Psychrobacter submarinus KMM 225T	151:183	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	1	44	attach	isolated	214:221	arg1	sample					240:245	a sea-water sample	228:245	a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean	228:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	1	44	attach	isolated	214:221	arg2	bacterium					204:212	a Gram-negative bacterium	188:212	a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean	188:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	2	45	from	225T	392:395	arg1	structure					328:336	the structure	324:336	the structure of the capsular polysaccharide from P. submarinus KMM 225T	324:395	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	2	45	from	225T	392:395	arg1	effect					405:410	its effect	401:410	its effect on the viability and colony formation of cancer cells	401:464	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	2	45	from	225T	392:395	arg1	polysaccharide					354:367	the capsular polysaccharide	341:367	the capsular polysaccharide from P. submarinus KMM 225T	341:395	Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells.					
33838818	4	46	theme	myelogenous	1109:1119	arg1	cells					1136:1140	the human chronic myelogenous leukemia K-562 cells	1091:1140	the human chronic myelogenous leukemia K-562 cells	1091:1140	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	0	47	from	bacterium	106:114	arg1	polysaccharide					73:86	the acidic capsular polysaccharide	53:86	the acidic capsular polysaccharide from the deep-sea bacterium	53:114	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	0	47	from	bacterium	106:114	arg1	activity					41:48	in vitro antiproliferative activity	14:48	in vitro antiproliferative activity	14:48	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	0	47	from	bacterium	106:114	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	4	48	theme	-d-glucose	758:767	arg1	S-Hb					782:785	S-Hb	782:785	S-Hb	782:785	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	48	theme	-d-glucose	758:767	arg1	[d-QuipNAc4N					769:780	2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6	704:867	[d-QuipNAc4N	769:780	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	48	theme	-d-glucose	758:767	arg1	S-Pyr					869:873	S-Pyr	869:873	S-Pyr	869:873	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	49	theme	chronic	1101:1107	arg1	cells					1136:1140	the human chronic myelogenous leukemia K-562 cells	1091:1140	the human chronic myelogenous leukemia K-562 cells	1091:1140	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	0	50	dep	in	14:15	arg1	vitro					17:21	vitro	17:21	vitro	17:21	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	0	51	dep	225T	145:148	arg1	activity					41:48	in vitro antiproliferative activity	14:48	in vitro antiproliferative activity	14:48	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	0	51	dep	225T	145:148	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	4	52	theme	K-562	1130:1134	arg1	cells					1136:1140	the human chronic myelogenous leukemia K-562 cells	1091:1140	the human chronic myelogenous leukemia K-562 cells	1091:1140	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	0	53	theme	capsular	64:71	arg1	polysaccharide					73:86	the acidic capsular polysaccharide	53:86	the acidic capsular polysaccharide from the deep-sea bacterium	53:114	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	3	54	theme	composition	608:618	arg1	analyses					620:627	composition analyses	608:627	composition analyses	608:627	The glycopolymer was purified by ultracentrifugation and chromatography methods, and the structure was elucidated using NMR spectroscopy and composition analyses.					
33838818	1	55	theme	Psychrobacter	151:163	arg1	bacterium					204:212	a Gram-negative bacterium	188:212	a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean	188:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	1	55	theme	Psychrobacter	151:163	arg1	225T					180:183	Psychrobacter submarinus KMM 225T	151:183	Psychrobacter submarinus KMM 225T	151:183	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	4	56	theme	leukemia	1121:1128	arg1	cells					1136:1140	the human chronic myelogenous leukemia K-562 cells	1091:1140	the human chronic myelogenous leukemia K-562 cells	1091:1140	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	0	57	theme	acidic	57:62	arg1	polysaccharide					73:86	the acidic capsular polysaccharide	53:86	the acidic capsular polysaccharide from the deep-sea bacterium	53:114	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T.					
33838818	4	58	theme	-1-carboxyethylidene	803:822	arg1	[d-GlcpNAc4,6					855:867	2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6	704:867	[d-GlcpNAc4,6	855:867	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	4	58	theme	-1-carboxyethylidene	803:822	arg1	S					801:801	S	801:801	S	801:801	The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.					
33838818	1	59	theme	submarinus	165:174	arg1	bacterium					204:212	a Gram-negative bacterium	188:212	a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean	188:306	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33838818	1	59	theme	submarinus	165:174	arg1	225T					180:183	Psychrobacter submarinus KMM 225T	151:183	Psychrobacter submarinus KMM 225T	151:183	Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean.					
33781812	0	0	theme	poly	64:67	arg1	microbeads					14:23	The composite microbeads	0:23	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.	0:165	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	0	theme	poly	64:67	arg1	characterization					106:121	characterization	106:121	characterization	106:121	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	0	theme	poly	64:67	arg1	Synthesis					95:103	Synthesis	95:103	Synthesis	95:103	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	1	1	theme	alginate	246:253	arg1	ACGP					221:224	ACGP	221:224	ACGP	221:224	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	1	theme	alginate	246:253	arg1	beads					237:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads	167:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P)	167:327	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	7	2	theme	Molecular	893:901	arg1	FMO					912:914	FMO	912:914	FMO	912:914	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	2	theme	Molecular	893:901	arg1	Orbital					903:909	The Frontier Molecular Orbital	880:909	The Frontier Molecular Orbital (FMO) analyses	880:924	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	2	3	contain	had	360:362	arg1	beads					354:358	The dried beads	344:358	The dried beads	344:358	The dried beads had a 700 μm average diameter.					
33781812	2	3	contain	had	360:362	arg2	diameter					381:388	a 700 μm average diameter	364:388	a 700 μm average diameter	364:388	The dried beads had a 700 μm average diameter.					
33781812	6	4	theme	FT-IR	865:869	arg1	spectra					871:877	the FT-IR spectra	861:877	the FT-IR spectra	861:877	The vibrational modes obtained by calculations were compared with those observed in the FT-IR spectra.					
33781812	7	5	theme	→	1245:1245	arg1	*					1248:1248	the n → σ*	1239:1248	the n → σ* (anomeric) interactions	1239:1272	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	6	theme	Orbital	903:909	arg1	analyses					917:924	The Frontier Molecular Orbital (FMO) analyses	880:924	The Frontier Molecular Orbital (FMO) analyses	880:924	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	1	7	theme	gelatin	277:283	arg1	ACGP					221:224	ACGP	221:224	ACGP	221:224	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	7	theme	gelatin	277:283	arg1	beads					237:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads	167:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P)	167:327	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	7	8	theme	σ	1247:1247	arg1	*					1248:1248	the n → σ*	1239:1248	the n → σ* (anomeric) interactions	1239:1272	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	0	9	theme	lactic-co-glycolic	69:86	arg1	poly					64:67	poly	64:67	poly(lactic-co-glycolic acid)	64:92	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	9	theme	lactic-co-glycolic	69:86	arg1	acid					88:91	lactic-co-glycolic acid	69:91	lactic-co-glycolic acid	69:91	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	5	10	theme	G	764:764	arg1	compounds					766:774	A, C, P, and G compounds	751:774	A, C, P, and G compounds	751:774	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	1	11	theme	poly	294:297	arg1	ACGP					221:224	ACGP	221:224	ACGP	221:224	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	11	theme	poly	294:297	arg1	beads					237:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads	167:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P)	167:327	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	5	12	theme	C	754:754	arg1	compounds					766:774	A, C, P, and G compounds	751:774	A, C, P, and G compounds	751:774	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	4	13	from	components	585:594	arg1	features					516:523	the features	512:523	the features of the composites	512:541	The results proved that the features of the composites were completely different from their bare components.					
33781812	4	13	from	components	585:594	arg1	different					559:567	different	559:567	different	559:567	The results proved that the features of the composites were completely different from their bare components.					
33781812	8	14	theme	drug	1506:1509	arg1	design					1511:1516	drug design	1506:1516	drug design	1506:1516	From the obtained results, these kinds of components can be hoped the promising materials for usage in the many scientific fields, especially in medicine and in drug design.					
33781812	7	15	theme	resonance	1115:1123	arg1	interactions					1126:1137	the n → П* (resonance) interactions	1103:1137	the n → П* (resonance) interactions for components A, G, and P	1103:1164	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	6	16	located	observed	849:856	arg1	spectra					871:877	the FT-IR spectra	861:877	the FT-IR spectra	861:877	The vibrational modes obtained by calculations were compared with those observed in the FT-IR spectra.					
33781812	6	16	located	observed	849:856	arg2	those					843:847	those	843:847	those	843:847	The vibrational modes obtained by calculations were compared with those observed in the FT-IR spectra.					
33781812	7	17	theme	Frontier	884:891	arg1	FMO					912:914	FMO	912:914	FMO	912:914	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	17	theme	Frontier	884:891	arg1	Orbital					903:909	The Frontier Molecular Orbital	880:909	The Frontier Molecular Orbital (FMO) analyses	880:924	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	18	theme	n	1243:1243	arg1	*					1248:1248	the n → σ*	1239:1248	the n → σ* (anomeric) interactions	1239:1272	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	2	19	theme	dried	348:352	arg1	beads					354:358	The dried beads	344:358	The dried beads	344:358	The dried beads had a 700 μm average diameter.					
33781812	8	20	from	materials	1425:1433	arg1	fields					1468:1473	the many scientific fields	1448:1473	the many scientific fields	1448:1473	From the obtained results, these kinds of components can be hoped the promising materials for usage in the many scientific fields, especially in medicine and in drug design.					
33781812	1	21	theme	lactic-co-glycolic	300:317	arg1	poly					294:297	poly	294:297	poly (lactic-co-glycolic acid) (P)	294:327	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	21	theme	lactic-co-glycolic	300:317	arg1	acid					319:322	lactic-co-glycolic acid	300:322	lactic-co-glycolic acid	300:322	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	4	22	theme	bare	580:583	arg1	components					585:594	their bare components	574:594	their bare components	574:594	The results proved that the features of the composites were completely different from their bare components.					
33781812	5	23	theme	*	678:678	arg1	level					681:685	the B3LYP/6-311++G** level	660:685	the B3LYP/6-311++G** level	660:685	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	7	24	theme	Bond	1067:1070	arg1	NBO					1054:1056	NBO	1054:1056	NBO	1054:1056	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	24	theme	Bond	1067:1070	arg1	Orbital					1072:1078	Natural Bond Orbital	1059:1078	Natural Bond Orbital	1059:1078	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	5	25	theme	Functional	605:614	arg1	calculations					629:640	Density Functional Theory (DFT) calculations	597:640	Density Functional Theory (DFT) calculations	597:640	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	1	26	theme	carrageenan	260:270	arg1	ACGP					221:224	ACGP	221:224	ACGP	221:224	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	26	theme	carrageenan	260:270	arg1	beads					237:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads	167:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P)	167:327	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	8	27	theme	components	1387:1396	arg1	kinds					1378:1382	these kinds	1372:1382	these kinds of components	1372:1396	From the obtained results, these kinds of components can be hoped the promising materials for usage in the many scientific fields, especially in medicine and in drug design.					
33781812	5	28	theme	B3LYP/6-311++G	664:677	arg1	level					681:685	the B3LYP/6-311++G** level	660:685	the B3LYP/6-311++G** level	660:685	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	0	29	theme	composite	4:12	arg1	microbeads					14:23	The composite microbeads	0:23	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.	0:165	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	29	theme	composite	4:12	arg1	characterization					106:121	characterization	106:121	characterization	106:121	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	29	theme	composite	4:12	arg1	Synthesis					95:103	Synthesis	95:103	Synthesis	95:103	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	4	30	theme	composites	532:541	arg1	features					516:523	the features	512:523	the features of the composites	512:541	The results proved that the features of the composites were completely different from their bare components.					
33781812	4	30	theme	composites	532:541	arg1	different					559:567	different	559:567	different	559:567	The results proved that the features of the composites were completely different from their bare components.					
33781812	1	31	theme	Binary	167:172	arg1	ACGP					221:224	ACGP	221:224	ACGP	221:224	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	31	theme	Binary	167:172	arg1	beads					237:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads	167:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P)	167:327	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	7	32	theme	component	942:950	arg1	G					952:952	the component G	938:952	the component G	938:952	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	32	theme	component	942:950	arg1	softer					962:967	softer	962:967	softer	962:967	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	33	theme	component	1334:1342	arg1	stabilization					1313:1325	the stabilization	1309:1325	the stabilization of the component	1309:1342	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	5	34	theme	*	679:679	arg1	level					681:685	the B3LYP/6-311++G** level	660:685	the B3LYP/6-311++G** level	660:685	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	5	35	theme	DFT	624:626	arg1	calculations					629:640	Density Functional Theory (DFT) calculations	597:640	Density Functional Theory (DFT) calculations	597:640	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	5	36	theme	compounds	766:774	arg1	properties					737:746	the elementary physical and chemical properties	700:746	the elementary physical and chemical properties of A, C, P, and G compounds	700:774	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	2	37	theme	average	373:379	arg1	diameter					381:388	a 700 μm average diameter	364:388	a 700 μm average diameter	364:388	The dried beads had a 700 μm average diameter.					
33781812	8	38	theme	scientific	1457:1466	arg1	fields					1468:1473	the many scientific fields	1448:1473	the many scientific fields	1448:1473	From the obtained results, these kinds of components can be hoped the promising materials for usage in the many scientific fields, especially in medicine and in drug design.					
33781812	5	39	theme	A	751:751	arg1	compounds					766:774	A, C, P, and G compounds	751:774	A, C, P, and G compounds	751:774	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	7	40	theme	→	1109:1109	arg1	interactions					1126:1137	the n → П* (resonance) interactions	1103:1137	the n → П* (resonance) interactions for components A, G, and P	1103:1164	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	2	41	theme	μm	370:371	arg1	diameter					381:388	a 700 μm average diameter	364:388	a 700 μm average diameter	364:388	The dried beads had a 700 μm average diameter.					
33781812	7	42	dep	*	1248:1248	arg1	anomeric					1251:1258	anomeric	1251:1258	anomeric	1251:1258	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	43	theme	NBO	1054:1056	arg1	analyses					1081:1088	component P. NBO (Natural Bond Orbital) analyses	1041:1088	component P. NBO (Natural Bond Orbital) analyses	1041:1088	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	1	44	dep	Binary	167:172	arg1	quaternary					209:218	quaternary	209:218	quaternary	209:218	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	44	dep	Binary	167:172	arg1	ternary					184:190	ternary	184:190	ternary	184:190	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	44	dep	Binary	167:172	arg1	AC					175:176	AC	175:176	AC	175:176	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	7	45	theme	Natural	1059:1065	arg1	NBO					1054:1056	NBO	1054:1056	NBO	1054:1056	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	45	theme	Natural	1059:1065	arg1	Orbital					1072:1078	Natural Bond Orbital	1059:1078	Natural Bond Orbital	1059:1078	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	0	46	theme	alginate	28:35	arg1	microbeads					14:23	The composite microbeads	0:23	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.	0:165	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	46	theme	alginate	28:35	arg1	characterization					106:121	characterization	106:121	characterization	106:121	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	46	theme	alginate	28:35	arg1	Synthesis					95:103	Synthesis	95:103	Synthesis	95:103	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	7	47	theme	stabilization	1211:1223	arg1	lowering					1185:1192	the lowering	1181:1192	the lowering of the molecular stabilization	1181:1223	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	48	theme	*	1248:1248	arg1	interactions					1261:1272	the n → σ* (anomeric) interactions	1239:1272	the n → σ* (anomeric) interactions	1239:1272	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	8	49	theme	promising	1415:1423	arg1	materials					1425:1433	the promising materials	1411:1433	the promising materials for usage in the many scientific fields	1411:1473	From the obtained results, these kinds of components can be hoped the promising materials for usage in the many scientific fields, especially in medicine and in drug design.					
33781812	7	50	theme	component	1041:1049	arg1	analyses					1081:1088	component P. NBO (Natural Bond Orbital) analyses	1041:1088	component P. NBO (Natural Bond Orbital) analyses	1041:1088	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	5	51	theme	Density	597:603	arg1	calculations					629:640	Density Functional Theory (DFT) calculations	597:640	Density Functional Theory (DFT) calculations	597:640	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	0	52	theme	Density	127:133	arg1	calculations					153:164	Density Functional Theory calculations	127:164	Density Functional Theory calculations	127:164	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	1	53	dep	ternary	184:190	arg1	ACP					198:200	ACP	198:200	ACP	198:200	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	53	dep	ternary	184:190	arg1	ACG					193:195	ACG	193:195	ACG	193:195	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	53	dep	ternary	184:190	arg1	AGP					203:205	AGP	203:205	AGP	203:205	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	5	54	theme	elementary	704:713	arg1	properties					737:746	the elementary physical and chemical properties	700:746	the elementary physical and chemical properties of A, C, P, and G compounds	700:774	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	0	55	theme	carrageenan	38:48	arg1	microbeads					14:23	The composite microbeads	0:23	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.	0:165	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	55	theme	carrageenan	38:48	arg1	characterization					106:121	characterization	106:121	characterization	106:121	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	55	theme	carrageenan	38:48	arg1	Synthesis					95:103	Synthesis	95:103	Synthesis	95:103	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	1	56	theme	composite	227:235	arg1	ACGP					221:224	ACGP	221:224	ACGP	221:224	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	1	56	theme	composite	227:235	arg1	beads					237:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads	167:241	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P)	167:327	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	3	57	theme	PZC	474:476	arg1	analysis					478:485	PZC analysis	474:485	PZC analysis	474:485	The microspheres with and without P were characterized by FT-IR, TGA/DTA, SEM, and PZC analysis.					
33781812	7	58	theme	n	1107:1107	arg1	interactions					1126:1137	the n → П* (resonance) interactions	1103:1137	the n → П* (resonance) interactions for components A, G, and P	1103:1164	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	5	59	theme	physical	715:722	arg1	properties					737:746	the elementary physical and chemical properties	700:746	the elementary physical and chemical properties of A, C, P, and G compounds	700:774	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	7	60	theme	other	1005:1009	arg1	components					1011:1020	the other components	1001:1020	the other components	1001:1020	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	0	61	theme	Theory	146:151	arg1	calculations					153:164	Density Functional Theory calculations	127:164	Density Functional Theory calculations	127:164	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	7	62	theme	molecular	1201:1209	arg1	stabilization					1211:1223	the molecular stabilization	1197:1223	the molecular stabilization	1197:1223	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	63	theme	П	1111:1111	arg1	interactions					1126:1137	the n → П* (resonance) interactions	1103:1137	the n → П* (resonance) interactions for components A, G, and P	1103:1164	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	64	dep	components	1143:1152	arg1	G					1157:1157	G	1157:1157	G	1157:1157	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	64	dep	components	1143:1152	arg1	P					1164:1164	P	1164:1164	P	1164:1164	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	64	dep	components	1143:1152	arg1	A					1154:1154	A	1154:1154	A	1154:1154	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	65	theme	*	1112:1112	arg1	interactions					1126:1137	the n → П* (resonance) interactions	1103:1137	the n → П* (resonance) interactions for components A, G, and P	1103:1164	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	0	66	theme	Functional	135:144	arg1	calculations					153:164	Density Functional Theory calculations	127:164	Density Functional Theory calculations	127:164	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	67	dep	microbeads	14:23	arg1	microbeads					14:23	The composite microbeads	0:23	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.	0:165	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	67	dep	microbeads	14:23	arg1	calculations					153:164	Density Functional Theory calculations	127:164	Density Functional Theory calculations	127:164	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	67	dep	microbeads	14:23	arg1	characterization					106:121	characterization	106:121	characterization	106:121	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	67	dep	microbeads	14:23	arg1	Synthesis					95:103	Synthesis	95:103	Synthesis	95:103	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	7	68	contain	had	973:975	arg2	gap					992:994	smaller energy gap	977:994	smaller energy gap	977:994	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	68	contain	had	973:975	arg1	G					952:952	the component G	938:952	the component G	938:952	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	7	68	contain	had	973:975	arg1	softer					962:967	softer	962:967	softer	962:967	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	5	69	theme	chemical	728:735	arg1	properties					737:746	the elementary physical and chemical properties	700:746	the elementary physical and chemical properties of A, C, P, and G compounds	700:774	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	7	70	theme	smaller	977:983	arg1	gap					992:994	smaller energy gap	977:994	smaller energy gap	977:994	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	5	71	theme	P	757:757	arg1	compounds					766:774	A, C, P, and G compounds	751:774	A, C, P, and G compounds	751:774	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33781812	1	72	dep	AC	175:176	arg1	AG					179:180	AG	179:180	AG	179:180	Binary (AC, AG), ternary (ACG, ACP, AGP), quaternary (ACGP) composite beads of alginate (A), carrageenan (C), gelatin (G), and poly (lactic-co-glycolic acid) (P) were prepared.					
33781812	8	73	theme	obtained	1354:1361	arg1	results					1363:1369	the obtained results	1350:1369	the obtained results	1350:1369	From the obtained results, these kinds of components can be hoped the promising materials for usage in the many scientific fields, especially in medicine and in drug design.					
33781812	7	74	theme	energy	985:990	arg1	gap					992:994	smaller energy gap	977:994	smaller energy gap	977:994	The Frontier Molecular Orbital (FMO) analyses showed that the component G was the softer and had smaller energy gap than the other components and vice versa for component P. NBO (Natural Bond Orbital) analyses implied that the n → П* (resonance) interactions for components A, G, and P contributed to the lowering of the molecular stabilization, whereas that the n → σ* (anomeric) interactions were responsible for decreasing of the stabilization of the component.					
33781812	6	75	theme	vibrational	781:791	arg1	modes					793:797	The vibrational modes	777:797	The vibrational modes obtained by calculations	777:822	The vibrational modes obtained by calculations were compared with those observed in the FT-IR spectra.					
33781812	0	76	theme	gelatin	51:57	arg1	microbeads					14:23	The composite microbeads	0:23	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.	0:165	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	76	theme	gelatin	51:57	arg1	characterization					106:121	characterization	106:121	characterization	106:121	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	0	76	theme	gelatin	51:57	arg1	Synthesis					95:103	Synthesis	95:103	Synthesis	95:103	The composite microbeads of alginate, carrageenan, gelatin, and poly(lactic-co-glycolic acid): Synthesis, characterization and Density Functional Theory calculations.					
33781812	8	77	theme	many	1452:1455	arg1	fields					1468:1473	the many scientific fields	1448:1473	the many scientific fields	1448:1473	From the obtained results, these kinds of components can be hoped the promising materials for usage in the many scientific fields, especially in medicine and in drug design.					
33781812	5	78	theme	Theory	616:621	arg1	calculations					629:640	Density Functional Theory (DFT) calculations	597:640	Density Functional Theory (DFT) calculations	597:640	Density Functional Theory (DFT) calculations were performed at the B3LYP/6-311++G** level to enlighten the elementary physical and chemical properties of A, C, P, and G compounds.					
33957047	5	0	theme	promising	1151:1159	arg1	method					1139:1144	the facile, environmentally friendly, and scalable fabrication method	1076:1144	the facile, environmentally friendly, and scalable fabrication method	1076:1144	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	0	theme	promising	1151:1159	arg1	strategy					1161:1168	a promising strategy	1149:1168	a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society	1149:1384	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	4	1	theme	29141	889:893	arg1	dB					895:896	dB	895:896	dB	895:896	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	1	2	theme	films	178:182	arg1	fabrication					153:163	The fabrication	149:163	The fabrication of ultrathin films that are electrically conductive and mechanically strong for electromagnetic interference (EMI) shielding applications	149:301	The fabrication of ultrathin films that are electrically conductive and mechanically strong for electromagnetic interference (EMI) shielding applications is challenging.					
33957047	1	3	theme	shielding	280:288	arg1	applications					290:301	electromagnetic interference (EMI) shielding applications	245:301	electromagnetic interference (EMI) shielding applications	245:301	The fabrication of ultrathin films that are electrically conductive and mechanically strong for electromagnetic interference (EMI) shielding applications is challenging.					
33957047	0	4	theme	Composite	72:80	arg1	Films					82:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films	0:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.	0:147	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.					
33957047	3	5	theme	wt	726:727	arg1	%					729:729	25.7 wt %	721:729	25.7 wt %	721:729	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	5	6	theme	facile	1080:1085	arg1	method					1139:1144	the facile, environmentally friendly, and scalable fabrication method	1076:1144	the facile, environmentally friendly, and scalable fabrication method	1076:1144	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	6	theme	facile	1080:1085	arg1	strategy					1161:1168	a promising strategy	1149:1168	a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society	1149:1384	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	2	7	theme	cellulose	389:397	arg1	composite					404:412	highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite	350:412	highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite	350:412	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	5	8	theme	shielding	1227:1235	arg1	materials					1237:1245	ultrathin, strong, and highly flexible EMI shielding materials	1184:1245	ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1184:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	8	theme	shielding	1227:1235	arg1	films					1292:1296	the freestanding Ti3C2T/BC composite films	1255:1296	the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1255:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	0	9	theme	High-Performance	92:107	arg1	Shielding					138:146	High-Performance Electromagnetic Interference Shielding	92:146	High-Performance Electromagnetic Interference Shielding	92:146	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.					
33957047	4	10	theme	cm2	898:900	arg1	g-1					902:904	29141 dB cm2 g-1	889:904	29141 dB cm2 g-1	889:904	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	2	11	theme	ultrathin	327:335	arg1	films					414:418	ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films	327:418	ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films	327:418	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	5	12	theme	efficient	1302:1310	arg1	shielding					1316:1324	efficient EMI shielding	1302:1324	efficient EMI shielding	1302:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	4	13	theme	polymer	969:975	arg1	composites					977:986	most previously reported MXene-based polymer composites	932:986	most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites	932:1050	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	4	14	theme	reported	948:955	arg1	composites					977:986	most previously reported MXene-based polymer composites	932:986	most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites	932:1050	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	5	15	theme	scalable	1118:1125	arg1	method					1139:1144	the facile, environmentally friendly, and scalable fabrication method	1076:1144	the facile, environmentally friendly, and scalable fabrication method	1076:1144	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	15	theme	scalable	1118:1125	arg1	strategy					1161:1168	a promising strategy	1149:1168	a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society	1149:1384	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	4	16	theme	dB	895:896	arg1	g-1					902:904	29141 dB cm2 g-1	889:904	29141 dB cm2 g-1	889:904	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	4	17	theme	thick	776:780	arg1	film					802:805	a 4 μm thick Ti3C2T/BC composite film	769:805	a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content	769:835	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	0	18	theme	Interference	125:136	arg1	Shielding					138:146	High-Performance Electromagnetic Interference Shielding	92:146	High-Performance Electromagnetic Interference Shielding	92:146	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.					
33957047	5	19	theme	fabrication	1127:1137	arg1	method					1139:1144	the facile, environmentally friendly, and scalable fabrication method	1076:1144	the facile, environmentally friendly, and scalable fabrication method	1076:1144	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	19	theme	fabrication	1127:1137	arg1	strategy					1161:1168	a promising strategy	1149:1168	a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society	1149:1384	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	20	theme	EMI	1223:1225	arg1	materials					1237:1245	ultrathin, strong, and highly flexible EMI shielding materials	1184:1245	ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1184:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	20	theme	EMI	1223:1225	arg1	films					1292:1296	the freestanding Ti3C2T/BC composite films	1255:1296	the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1255:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	0	21	theme	Electromagnetic	109:123	arg1	Shielding					138:146	High-Performance Electromagnetic Interference Shielding	92:146	High-Performance Electromagnetic Interference Shielding	92:146	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.					
33957047	4	22	theme	specific	852:859	arg1	EMI					861:863	a specific EMI	850:863	a specific EMI	850:863	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	4	23	theme	composite	792:800	arg1	film					802:805	a 4 μm thick Ti3C2T/BC composite film	769:805	a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content	769:835	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	5	24	theme	society	1378:1384	arg1	problems					1341:1348	EMI problems	1337:1348	EMI problems of a fast-developing modern society	1337:1384	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	4	25	theme	MXene	1001:1005	arg1	contents					1007:1014	similar MXene contents	993:1014	similar MXene contents	993:1014	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	4	26	theme	similar	993:999	arg1	contents					1007:1014	similar MXene contents	993:1014	similar MXene contents	993:1014	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	4	27	theme	μm	773:774	arg1	film					802:805	a 4 μm thick Ti3C2T/BC composite film	769:805	a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content	769:835	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	5	28	theme	strong	1195:1200	arg1	materials					1237:1245	ultrathin, strong, and highly flexible EMI shielding materials	1184:1245	ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1184:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	28	theme	strong	1195:1200	arg1	films					1292:1296	the freestanding Ti3C2T/BC composite films	1255:1296	the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1255:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	0	29	theme	Ultrathin	0:8	arg1	Films					82:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films	0:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.	0:147	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.					
33957047	4	30	with	film	802:805	arg1	content					829:835	76.9 wt % Ti3C2T content	812:835	76.9 wt % Ti3C2T content	812:835	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	5	31	theme	EMI	1337:1339	arg1	problems					1341:1348	EMI problems	1337:1348	EMI problems of a fast-developing modern society	1337:1384	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	2	32	theme	composite	404:412	arg1	films					414:418	ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films	327:418	ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films	327:418	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	2	33	theme	biosynthesis	457:468	arg1	method					470:475	a scalable in situ biosynthesis method	438:475	a scalable in situ biosynthesis method	438:475	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	2	34	theme	strong	338:343	arg1	ultrathin					327:335	ultrathin	327:335	ultrathin	327:335	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	2	35	theme	in	449:450	arg1	method					470:475	a scalable in situ biosynthesis method	438:475	a scalable in situ biosynthesis method	438:475	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	0	36	theme	Strong	11:16	arg1	Films					82:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films	0:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.	0:147	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.					
33957047	3	37	theme	three-dimensional	537:553	arg1	network					558:564	the three-dimensional BC network	533:564	the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility	533:753	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	2	38	theme	scalable	440:447	arg1	method					470:475	a scalable in situ biosynthesis method	438:475	a scalable in situ biosynthesis method	438:475	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	4	39	theme	carbon-based	1020:1031	arg1	composites					1041:1050	carbon-based polymer composites	1020:1050	carbon-based polymer composites	1020:1050	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	5	40	theme	flexible	1214:1221	arg1	materials					1237:1245	ultrathin, strong, and highly flexible EMI shielding materials	1184:1245	ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1184:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	40	theme	flexible	1214:1221	arg1	films					1292:1296	the freestanding Ti3C2T/BC composite films	1255:1296	the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1255:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	41	theme	friendly	1104:1111	arg1	method					1139:1144	the facile, environmentally friendly, and scalable fabrication method	1076:1144	the facile, environmentally friendly, and scalable fabrication method	1076:1144	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	41	theme	friendly	1104:1111	arg1	strategy					1161:1168	a promising strategy	1149:1168	a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society	1149:1384	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	3	42	theme	composite	634:642	arg1	films					644:648	the MXene/BC composite films	621:648	the MXene/BC composite films	621:648	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	3	43	from	%	729:729	arg1	strength					696:703	tensile strength	688:703	tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T	688:736	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	3	43	from	%	729:729	arg1	Ti3C2T					731:736	Ti3C2T	731:736	Ti3C2T	731:736	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	4	44	theme	polymer	1033:1039	arg1	composites					1041:1050	carbon-based polymer composites	1020:1050	carbon-based polymer composites	1020:1050	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	4	45	theme	Ti3C2T	822:827	arg1	content					829:835	76.9 wt % Ti3C2T content	812:835	76.9 wt % Ti3C2T content	812:835	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	1	46	theme	electromagnetic	245:259	arg1	applications					290:301	electromagnetic interference (EMI) shielding applications	245:301	electromagnetic interference (EMI) shielding applications	245:301	The fabrication of ultrathin films that are electrically conductive and mechanically strong for electromagnetic interference (EMI) shielding applications is challenging.					
33957047	0	47	theme	Ti3C2T	39:44	arg1	Films					82:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films	0:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.	0:147	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.					
33957047	4	48	theme	MXene-based	957:967	arg1	composites					977:986	most previously reported MXene-based polymer composites	932:986	most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites	932:1050	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	3	49	theme	tensile	688:694	arg1	strength					696:703	tensile strength	688:703	tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T	688:736	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	3	49	theme	tensile	688:694	arg1	Ti3C2T					731:736	Ti3C2T	731:736	Ti3C2T	731:736	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	2	50	dep	in	449:450	arg1	situ					452:455	situ	452:455	situ	452:455	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	2	51	theme	MXene/bacterial	373:387	arg1	composite					404:412	highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite	350:412	highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite	350:412	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	0	52	theme	Flexible	30:37	arg1	Films					82:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films	0:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.	0:147	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.					
33957047	4	53	theme	g-1	902:904	arg1	efficiency					875:884	efficiency	875:884	efficiency of 29141 dB cm2 g-1	875:904	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	3	54	theme	MXene/BC	625:632	arg1	films					644:648	the MXene/BC composite films	621:648	the MXene/BC composite films	621:648	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	2	55	theme	Ti3C2T	366:371	arg1	composite					404:412	highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite	350:412	highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite	350:412	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	5	56	theme	EMI	1312:1314	arg1	shielding					1316:1324	efficient EMI shielding	1302:1324	efficient EMI shielding	1302:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	4	57	theme	wt	817:818	arg1	Ti3C2T					822:827	76.9 wt % Ti3C2T	812:827	76.9 wt % Ti3C2T content	812:835	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	3	58	theme	mechanical	665:674	arg1	properties					676:685	excellent mechanical properties	655:685	excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T)	655:737	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	2	59	theme	flexible	357:364	arg1	composite					404:412	highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite	350:412	highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite	350:412	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	3	60	theme	entangled	589:597	arg1	structure					599:607	a mechanically entangled structure	574:607	a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility	574:753	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	3	61	dep	properties	676:685	arg1	strength					696:703	tensile strength	688:703	tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T	688:736	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	3	61	dep	properties	676:685	arg1	Ti3C2T					731:736	Ti3C2T	731:736	Ti3C2T	731:736	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	4	62	theme	Ti3C2T/BC	782:790	arg1	film					802:805	a 4 μm thick Ti3C2T/BC composite film	769:805	a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content	769:835	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	4	63	with	composites	977:986	arg1	composites					1041:1050	carbon-based polymer composites	1020:1050	carbon-based polymer composites	1020:1050	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	4	63	with	composites	977:986	arg1	contents					1007:1014	similar MXene contents	993:1014	similar MXene contents	993:1014	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	1	64	theme	ultrathin	168:176	arg1	films					178:182	ultrathin films	168:182	ultrathin films that are electrically conductive and mechanically strong for electromagnetic interference (EMI) shielding applications	168:301	The fabrication of ultrathin films that are electrically conductive and mechanically strong for electromagnetic interference (EMI) shielding applications is challenging.					
33957047	0	65	theme	Cellulose	62:70	arg1	Films					82:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films	0:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.	0:147	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.					
33957047	3	66	theme	MPa	714:716	arg1	strength					696:703	tensile strength	688:703	tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T	688:736	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	3	66	theme	MPa	714:716	arg1	Ti3C2T					731:736	Ti3C2T	731:736	Ti3C2T	731:736	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	4	67	theme	%	820:820	arg1	Ti3C2T					822:827	76.9 wt % Ti3C2T	812:827	76.9 wt % Ti3C2T content	812:835	Importantly, a 4 μm thick Ti3C2T/BC composite film with 76.9 wt % Ti3C2T content demonstrates a specific EMI shielding efficiency of 29141 dB cm2 g-1, which surpasses those of most previously reported MXene-based polymer composites with similar MXene contents and carbon-based polymer composites.					
33957047	3	68	theme	MXene	489:493	arg1	nanosheets					495:504	The Ti3C2T MXene nanosheets	478:504	The Ti3C2T MXene nanosheets	478:504	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	3	69	theme	BC	555:556	arg1	network					558:564	the three-dimensional BC network	533:564	the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility	533:753	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	5	70	theme	freestanding	1259:1270	arg1	films					1292:1296	the freestanding Ti3C2T/BC composite films	1255:1296	the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1255:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	0	71	theme	MXene/Bacterial	46:60	arg1	Films					82:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films	0:86	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.	0:147	Ultrathin, Strong, and Highly Flexible Ti3C2T MXene/Bacterial Cellulose Composite Films for High-Performance Electromagnetic Interference Shielding.					
33957047	5	72	theme	ultrathin	1184:1192	arg1	materials					1237:1245	ultrathin, strong, and highly flexible EMI shielding materials	1184:1245	ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1184:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	72	theme	ultrathin	1184:1192	arg1	films					1292:1296	the freestanding Ti3C2T/BC composite films	1255:1296	the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1255:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	3	73	theme	excellent	655:663	arg1	properties					676:685	excellent mechanical properties	655:685	excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T)	655:737	The Ti3C2T MXene nanosheets are uniformly dispersed in the three-dimensional BC network to form a mechanically entangled structure that endows the MXene/BC composite films with excellent mechanical properties (tensile strength of 297.5 MPa at 25.7 wt % Ti3C2T) and flexibility.					
33957047	2	74	theme	BC	400:401	arg1	composite					404:412	highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite	350:412	highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite	350:412	Herein, ultrathin, strong, and highly flexible Ti3C2T MXene/bacterial cellulose (BC) composite films are fabricated by a scalable in situ biosynthesis method.					
33957047	5	75	theme	composite	1282:1290	arg1	films					1292:1296	the freestanding Ti3C2T/BC composite films	1255:1296	the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1255:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
33957047	5	76	theme	Ti3C2T/BC	1272:1280	arg1	films					1292:1296	the freestanding Ti3C2T/BC composite films	1255:1296	the freestanding Ti3C2T/BC composite films for efficient EMI shielding	1255:1324	Our findings show that the facile, environmentally friendly, and scalable fabrication method is a promising strategy for producing ultrathin, strong, and highly flexible EMI shielding materials such as the freestanding Ti3C2T/BC composite films for efficient EMI shielding to address EMI problems of a fast-developing modern society.					
32070514	2	0	theme	control	498:504	arg1	groups					506:511	control groups	498:511	control groups	498:511	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	1	1	theme	fermentation	163:174	arg1	behaviour					176:184	The fermentation behaviour	159:184	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis	159:295	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis were examined.					
32070514	0	2	theme	agaro-oligosaccharides	83:104	arg1	fermentation					9:20	In vitro fermentation	0:20	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.	0:157	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	7	3	from	sources	1197:1203	arg1	foods					1233:1237	functional foods	1222:1237	functional foods	1222:1237	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	4	4	theme	bacteria	719:726	arg1	abundances					684:693	the abundances	680:693	the abundances of potential pathogenic bacteria	680:726	Moreover, the abundances of potential pathogenic bacteria were reduced.					
32070514	0	5	theme	fecal	115:119	arg1	inocula					121:127	human fecal inocula	109:127	human fecal inocula	109:127	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	3	6	theme	Firmicutes	639:648	arg1	abundance					626:634	the abundance	622:634	the abundance of Firmicutes and Actinobacteria	622:667	GLP increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes, while GLO increased the abundance of Firmicutes and Actinobacteria.					
32070514	0	7	theme	human	109:113	arg1	inocula					121:127	human fecal inocula	109:127	human fecal inocula	109:127	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	7	8	from	prebiotics	1208:1217	arg1	foods					1233:1237	functional foods	1222:1237	functional foods	1222:1237	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	6	9	theme	polymerization	935:948	arg1	degree					925:930	smaller degree	917:930	smaller degree of polymerization of oligosaccharides	917:968	Furthermore, GLP was degraded into smaller degree of polymerization of oligosaccharides, with no significant change observed in GLO.					
32070514	2	10	dep	composition	436:446	arg1	the					432:434	the	432:434	the	432:434	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	2	11	theme	fatty	398:402	arg1	acids					404:408	short chain fatty acids	386:408	short chain fatty acids (SCFAs)	386:416	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	2	11	theme	fatty	398:402	arg1	SCFAs					411:415	SCFAs	411:415	SCFAs	411:415	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	3	12	theme	Bacteroidetes	545:557	arg1	abundance					532:540	the abundance	528:540	the abundance of Bacteroidetes	528:557	GLP increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes, while GLO increased the abundance of Firmicutes and Actinobacteria.					
32070514	2	13	theme	acids	404:408	arg1	concentrations					368:381	the concentrations	364:381	the concentrations of short chain fatty acids (SCFAs)	364:416	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	5	14	dep	1.22 × 105	833:842	arg1	to					830:831	to	830:831	to	830:831	Molecular weight and intrinsic viscosity of GLP decreased significantly from 2.15 × 105 to 1.22 × 105 Da, 374.45-113.91 mL/g, respectively.					
32070514	7	15	from	foods	1233:1237	arg1	sources					1197:1203	sources	1197:1203	sources of prebiotics in functional foods	1197:1237	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	7	15	from	foods	1233:1237	arg1	GLP					1169:1171	GLP	1169:1171	GLP	1169:1171	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	7	15	from	foods	1233:1237	arg1	GLO					1177:1179	GLO	1177:1179	GLO	1177:1179	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	7	16	theme	functional	1222:1231	arg1	foods					1233:1237	functional foods	1222:1237	functional foods	1222:1237	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	1	17	theme	polysaccharides	198:212	arg1	behaviour					176:184	The fermentation behaviour	159:184	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis	159:295	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis were examined.					
32070514	0	18	theme	In	0:1	arg1	fermentation					9:20	In vitro fermentation	0:20	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.	0:157	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	7	19	theme	intestinal	1137:1146	arg1	microbes					1148:1155	intestinal microbes	1137:1155	intestinal microbes	1137:1155	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	3	20	theme	Firmicutes	590:599	arg1	abundance					577:585	the abundance	573:585	the abundance of Firmicutes	573:599	GLP increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes, while GLO increased the abundance of Firmicutes and Actinobacteria.					
32070514	2	21	theme	chain	392:396	arg1	acids					404:408	short chain fatty acids	386:408	short chain fatty acids (SCFAs)	386:416	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	2	21	theme	chain	392:396	arg1	SCFAs					411:415	SCFAs	411:415	SCFAs	411:415	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	7	22	theme	prebiotics	1208:1217	arg1	sources					1197:1203	sources	1197:1203	sources of prebiotics in functional foods	1197:1237	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	7	22	theme	prebiotics	1208:1217	arg1	GLP					1169:1171	GLP	1169:1171	GLP	1169:1171	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	7	22	theme	prebiotics	1208:1217	arg1	GLO					1177:1179	GLO	1177:1179	GLO	1177:1179	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	6	23	theme	oligosaccharides	953:968	arg1	polymerization					935:948	polymerization	935:948	polymerization of oligosaccharides	935:968	Furthermore, GLP was degraded into smaller degree of polymerization of oligosaccharides, with no significant change observed in GLO.					
32070514	6	24	theme	smaller	917:923	arg1	degree					925:930	smaller degree	917:930	smaller degree of polymerization of oligosaccharides	917:968	Furthermore, GLP was degraded into smaller degree of polymerization of oligosaccharides, with no significant change observed in GLO.					
32070514	2	25	theme	in	319:320	arg1	fermentation					328:339	in vitro fermentation	319:339	in vitro fermentation	319:339	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	4	26	theme	pathogenic	708:717	arg1	bacteria					719:726	potential pathogenic bacteria	698:726	potential pathogenic bacteria	698:726	Moreover, the abundances of potential pathogenic bacteria were reduced.					
32070514	0	27	theme	lemaneiformis	36:48	arg1	polysaccharides					59:73	Gracilaria lemaneiformis sulfated polysaccharides	25:73	Gracilaria lemaneiformis sulfated polysaccharides	25:73	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	5	28	theme	Molecular	742:750	arg1	weight					752:757	Molecular weight	742:757	Molecular weight	742:757	Molecular weight and intrinsic viscosity of GLP decreased significantly from 2.15 × 105 to 1.22 × 105 Da, 374.45-113.91 mL/g, respectively.					
32070514	6	29	located	observed	998:1005	arg2	change					991:996	no significant change	976:996	no significant change observed in GLO	976:1012	Furthermore, GLP was degraded into smaller degree of polymerization of oligosaccharides, with no significant change observed in GLO.					
32070514	6	29	located	observed	998:1005	arg1	GLO					1010:1012	GLO	1010:1012	GLO	1010:1012	Furthermore, GLP was degraded into smaller degree of polymerization of oligosaccharides, with no significant change observed in GLO.					
32070514	0	30	theme	Gracilaria	25:34	arg1	polysaccharides					59:73	Gracilaria lemaneiformis sulfated polysaccharides	25:73	Gracilaria lemaneiformis sulfated polysaccharides	25:73	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	2	31	theme	microorganisms	469:482	arg1	diversity					452:460	diversity	452:460	diversity	452:460	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	2	31	theme	microorganisms	469:482	arg1	composition					436:446	composition	436:446	composition	436:446	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	0	32	theme	polysaccharides	59:73	arg1	fermentation					9:20	In vitro fermentation	0:20	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.	0:157	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	7	33	theme	gastrointestinal	1080:1095	arg1	tract					1097:1101	gastrointestinal tract	1080:1101	gastrointestinal tract	1080:1101	Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.					
32070514	3	34	theme	Actinobacteria	654:667	arg1	abundance					626:634	the abundance	622:634	the abundance of Firmicutes and Actinobacteria	622:667	GLP increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes, while GLO increased the abundance of Firmicutes and Actinobacteria.					
32070514	0	35	theme	sulfated	50:57	arg1	polysaccharides					59:73	Gracilaria lemaneiformis sulfated polysaccharides	25:73	Gracilaria lemaneiformis sulfated polysaccharides	25:73	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	2	36	dep	in	319:320	arg1	vitro					322:326	vitro	322:326	vitro	322:326	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	6	37	theme	significant	979:989	arg1	change					991:996	no significant change	976:996	no significant change observed in GLO	976:1012	Furthermore, GLP was degraded into smaller degree of polymerization of oligosaccharides, with no significant change observed in GLO.					
32070514	0	38	from	inocula	121:127	arg1	microbiota					147:156	microbiota	147:156	microbiota	147:156	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	1	39	theme	agaro-oligosaccharides	230:251	arg1	behaviour					176:184	The fermentation behaviour	159:184	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis	159:295	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis were examined.					
32070514	1	40	attach	derived	259:265	arg1	lemaneiformis					283:295	Gracilaria lemaneiformis	272:295	Gracilaria lemaneiformis	272:295	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis were examined.					
32070514	1	40	attach	derived	259:265	arg2	GLP					215:217	GLP	215:217	GLP	215:217	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis were examined.					
32070514	1	40	attach	derived	259:265	arg2	polysaccharides					198:212	sulfated polysaccharides	189:212	sulfated polysaccharides (GLP)	189:218	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis were examined.					
32070514	5	41	theme	GLP	786:788	arg1	viscosity					773:781	intrinsic viscosity	763:781	intrinsic viscosity	763:781	Molecular weight and intrinsic viscosity of GLP decreased significantly from 2.15 × 105 to 1.22 × 105 Da, 374.45-113.91 mL/g, respectively.					
32070514	5	41	theme	GLP	786:788	arg1	weight					752:757	Molecular weight	742:757	Molecular weight	742:757	Molecular weight and intrinsic viscosity of GLP decreased significantly from 2.15 × 105 to 1.22 × 105 Da, 374.45-113.91 mL/g, respectively.					
32070514	2	42	theme	short	386:390	arg1	acids					404:408	short chain fatty acids	386:408	short chain fatty acids (SCFAs)	386:416	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	2	42	theme	short	386:390	arg1	SCFAs					411:415	SCFAs	411:415	SCFAs	411:415	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	1	43	theme	sulfated	189:196	arg1	polysaccharides					198:212	sulfated polysaccharides	189:212	sulfated polysaccharides (GLP)	189:218	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis were examined.					
32070514	1	43	theme	sulfated	189:196	arg1	GLP					215:217	GLP	215:217	GLP	215:217	The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis were examined.					
32070514	0	44	from	impact	137:142	arg1	microbiota					147:156	microbiota	147:156	microbiota	147:156	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	2	45	theme	gut	465:467	arg1	microorganisms					469:482	gut microorganisms	465:482	gut microorganisms	465:482	During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups.					
32070514	0	46	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.					
32070514	4	47	theme	potential	698:706	arg1	bacteria					719:726	potential pathogenic bacteria	698:726	potential pathogenic bacteria	698:726	Moreover, the abundances of potential pathogenic bacteria were reduced.					
32070514	5	48	theme	intrinsic	763:771	arg1	viscosity					773:781	intrinsic viscosity	763:781	intrinsic viscosity	763:781	Molecular weight and intrinsic viscosity of GLP decreased significantly from 2.15 × 105 to 1.22 × 105 Da, 374.45-113.91 mL/g, respectively.					
34420749	3	0	theme	immature	197:204	arg1	endosperm					206:214	(BF) immature endosperm	192:214	(BF) immature endosperm	192:214	(BF) immature endosperm was decellularized to produce three dimensional (3D) cellulose scaffolds that can support mammalian 3D cell culture.					
34420749	6	1	theme	cellulose-chitosan	733:750	arg1	scaffolds					768:776	cellulose-chitosan (CS/CHI) hybrid scaffolds	733:776	cellulose-chitosan (CS/CHI) hybrid scaffolds	733:776	Furthermore cellulosic scaffolds (CS) obtained from BF was treated with chitosan to produce cellulose-chitosan (CS/CHI) hybrid scaffolds.					
34420749	6	2	theme	cellulosic	653:662	arg1	CS					675:676	CS	675:676	CS	675:676	Furthermore cellulosic scaffolds (CS) obtained from BF was treated with chitosan to produce cellulose-chitosan (CS/CHI) hybrid scaffolds.					
34420749	6	2	theme	cellulosic	653:662	arg1	scaffolds					664:672	cellulosic scaffolds	653:672	cellulosic scaffolds (CS) obtained from BF	653:694	Furthermore cellulosic scaffolds (CS) obtained from BF was treated with chitosan to produce cellulose-chitosan (CS/CHI) hybrid scaffolds.					
34420749	7	3	from	investigation	795:807	arg1	scaffolds					817:825	both scaffolds	812:825	both scaffolds	812:825	The comparative investigation on both scaffolds exhibited adequate swelling with controlled porosity and pore-size distribution.					
34420749	5	4	theme	intrinsic	561:569	arg1	phyto-ingredients					571:587	intrinsic phyto-ingredients	561:587	intrinsic phyto-ingredients which provide excellent pharmacological properties	561:638	The results demonstrated that the BF tissue represented a complex concoction of polysaccharides with intrinsic phyto-ingredients which provide excellent pharmacological properties.					
34420749	5	5	theme	excellent	603:611	arg1	properties					629:638	excellent pharmacological properties	603:638	excellent pharmacological properties	603:638	The results demonstrated that the BF tissue represented a complex concoction of polysaccharides with intrinsic phyto-ingredients which provide excellent pharmacological properties.					
34420749	10	6	link	derived	1229:1235	arg1	material					1302:1309	an alternative scaffolding material	1275:1309	an alternative scaffolding material in regenerative medicine	1275:1334	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	10	6	link	derived	1229:1235	arg1	scaffolds					1248:1256	plant derived cellulosic scaffolds	1223:1256	plant derived cellulosic scaffolds	1223:1256	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	8	7	theme	CS/CHI	1039:1044	arg1	group					1046:1050	CS/CHI group	1039:1050	CS/CHI group	1039:1050	The physiochemical characterization showed reduced biodegradation, improved thermal stability and enhanced compressive strength in CS/CHI group.					
34420749	10	8	theme	regenerative	1314:1325	arg1	medicine					1327:1334	regenerative medicine	1314:1334	regenerative medicine	1314:1334	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	4	9	theme	endosperm	449:457	arg1	composition					381:391	the chemical composition	368:391	the chemical composition	368:391	To this regard, we first evaluated the chemical composition, nutritive profile and pharmacological activities of BF endosperm.					
34420749	4	9	theme	endosperm	449:457	arg1	profile					404:410	nutritive profile	394:410	nutritive profile	394:410	To this regard, we first evaluated the chemical composition, nutritive profile and pharmacological activities of BF endosperm.					
34420749	4	9	theme	endosperm	449:457	arg1	activities					432:441	pharmacological activities	416:441	pharmacological activities	416:441	To this regard, we first evaluated the chemical composition, nutritive profile and pharmacological activities of BF endosperm.					
34420749	4	10	theme	chemical	372:379	arg1	composition					381:391	the chemical composition	368:391	the chemical composition	368:391	To this regard, we first evaluated the chemical composition, nutritive profile and pharmacological activities of BF endosperm.					
34420749	5	11	theme	pharmacological	613:627	arg1	properties					629:638	excellent pharmacological properties	603:638	excellent pharmacological properties	603:638	The results demonstrated that the BF tissue represented a complex concoction of polysaccharides with intrinsic phyto-ingredients which provide excellent pharmacological properties.					
34420749	5	12	with	concoction	526:535	arg1	phyto-ingredients					571:587	intrinsic phyto-ingredients	561:587	intrinsic phyto-ingredients which provide excellent pharmacological properties	561:638	The results demonstrated that the BF tissue represented a complex concoction of polysaccharides with intrinsic phyto-ingredients which provide excellent pharmacological properties.					
34420749	3	13	theme	cell	319:322	arg1	culture					324:330	mammalian 3D cell culture	306:330	mammalian 3D cell culture	306:330	(BF) immature endosperm was decellularized to produce three dimensional (3D) cellulose scaffolds that can support mammalian 3D cell culture.					
34420749	4	14	theme	BF	446:447	arg1	endosperm					449:457	BF endosperm	446:457	BF endosperm	446:457	To this regard, we first evaluated the chemical composition, nutritive profile and pharmacological activities of BF endosperm.					
34420749	6	15	theme	hybrid	761:766	arg1	scaffolds					768:776	cellulose-chitosan (CS/CHI) hybrid scaffolds	733:776	cellulose-chitosan (CS/CHI) hybrid scaffolds	733:776	Furthermore cellulosic scaffolds (CS) obtained from BF was treated with chitosan to produce cellulose-chitosan (CS/CHI) hybrid scaffolds.					
34420749	8	16	theme	physiochemical	912:925	arg1	characterization					927:942	The physiochemical characterization	908:942	The physiochemical characterization	908:942	The physiochemical characterization showed reduced biodegradation, improved thermal stability and enhanced compressive strength in CS/CHI group.					
34420749	10	17	theme	scaffolding	1290:1300	arg1	scaffolds					1248:1256	plant derived cellulosic scaffolds	1223:1256	plant derived cellulosic scaffolds	1223:1256	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	10	17	theme	scaffolding	1290:1300	arg1	material					1302:1309	an alternative scaffolding material	1275:1309	an alternative scaffolding material in regenerative medicine	1275:1334	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	9	18	theme	Biological	1053:1062	arg1	studies					1064:1070	Biological studies	1053:1070	Biological studies	1053:1070	Biological studies reported favorable adhesion and proliferation of fibroblasts with evident cellular penetration and colonization on the both scaffolds.					
34420749	7	19	theme	pore-size	884:892	arg1	distribution					894:905	pore-size distribution	884:905	pore-size distribution	884:905	The comparative investigation on both scaffolds exhibited adequate swelling with controlled porosity and pore-size distribution.					
34420749	0	20	theme	natural	15:21	arg1	scaffold					36:43	Decellularized natural 3D cellulose scaffold	0:43	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)	0:85	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)					
34420749	9	21	with	fibroblasts	1121:1131	arg1	penetration					1155:1165	evident cellular penetration	1138:1165	evident cellular penetration	1138:1165	Biological studies reported favorable adhesion and proliferation of fibroblasts with evident cellular penetration and colonization on the both scaffolds.					
34420749	9	21	with	fibroblasts	1121:1131	arg1	colonization					1171:1182	colonization	1171:1182	colonization	1171:1182	Biological studies reported favorable adhesion and proliferation of fibroblasts with evident cellular penetration and colonization on the both scaffolds.					
34420749	0	22	attach	derived	45:51	arg1	Linn					80:83	Linn	80:83	Linn	80:83	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)					
34420749	0	22	attach	derived	45:51	arg1	flabellifer					67:77	flabellifer	67:77	flabellifer	67:77	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)					
34420749	0	22	attach	derived	45:51	arg2	scaffold					36:43	Decellularized natural 3D cellulose scaffold	0:43	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)	0:85	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)					
34420749	0	23	theme	Decellularized	0:13	arg1	scaffold					36:43	Decellularized natural 3D cellulose scaffold	0:43	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)	0:85	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)					
34420749	7	24	theme	adequate	837:844	arg1	swelling					846:853	adequate swelling	837:853	adequate swelling	837:853	The comparative investigation on both scaffolds exhibited adequate swelling with controlled porosity and pore-size distribution.					
34420749	8	25	dep	showed	944:949	arg1	enhanced					1006:1013	enhanced	1006:1013	enhanced compressive strength in CS/CHI group	1006:1050	The physiochemical characterization showed reduced biodegradation, improved thermal stability and enhanced compressive strength in CS/CHI group.					
34420749	8	25	dep	showed	944:949	arg1	reduced					951:957	reduced	951:957	reduced biodegradation	951:972	The physiochemical characterization showed reduced biodegradation, improved thermal stability and enhanced compressive strength in CS/CHI group.					
34420749	8	25	dep	showed	944:949	arg1	improved					975:982	improved	975:982	improved thermal stability	975:1000	The physiochemical characterization showed reduced biodegradation, improved thermal stability and enhanced compressive strength in CS/CHI group.					
34420749	0	26	theme	cellulose	26:34	arg1	scaffold					36:43	Decellularized natural 3D cellulose scaffold	0:43	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)	0:85	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)					
34420749	10	27	theme	cellulosic	1237:1246	arg1	material					1302:1309	an alternative scaffolding material	1275:1309	an alternative scaffolding material in regenerative medicine	1275:1334	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	10	27	theme	cellulosic	1237:1246	arg1	scaffolds					1248:1256	plant derived cellulosic scaffolds	1223:1256	plant derived cellulosic scaffolds	1223:1256	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	1	28	theme	extracellular	90:102	arg1	matrix					104:109	extracellular matrix	90:109	extracellular matrix for tissue engineering applications	90:145	as extracellular matrix for tissue engineering applications.					
34420749	0	29	theme	3D	23:24	arg1	scaffold					36:43	Decellularized natural 3D cellulose scaffold	0:43	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)	0:85	Decellularized natural 3D cellulose scaffold derived from Borassus flabellifer (Linn.)					
34420749	10	30	theme	derived	1229:1235	arg1	material					1302:1309	an alternative scaffolding material	1275:1309	an alternative scaffolding material in regenerative medicine	1275:1334	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	10	30	theme	derived	1229:1235	arg1	scaffolds					1248:1256	plant derived cellulosic scaffolds	1223:1256	plant derived cellulosic scaffolds	1223:1256	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	4	31	theme	pharmacological	416:430	arg1	activities					432:441	pharmacological activities	416:441	pharmacological activities	416:441	To this regard, we first evaluated the chemical composition, nutritive profile and pharmacological activities of BF endosperm.					
34420749	8	32	theme	thermal	984:990	arg1	stability					992:1000	thermal stability	984:1000	thermal stability	984:1000	The physiochemical characterization showed reduced biodegradation, improved thermal stability and enhanced compressive strength in CS/CHI group.					
34420749	9	33	theme	favorable	1081:1089	arg1	adhesion					1091:1098	favorable adhesion	1081:1098	favorable adhesion	1081:1098	Biological studies reported favorable adhesion and proliferation of fibroblasts with evident cellular penetration and colonization on the both scaffolds.					
34420749	10	34	from	material	1302:1309	arg1	medicine					1327:1334	regenerative medicine	1314:1334	regenerative medicine	1314:1334	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	7	35	theme	controlled	860:869	arg1	porosity					871:878	controlled porosity	860:878	controlled porosity	860:878	The comparative investigation on both scaffolds exhibited adequate swelling with controlled porosity and pore-size distribution.					
34420749	3	36	theme	dimensional	252:262	arg1	scaffolds					279:287	three dimensional (3D) cellulose scaffolds	246:287	three dimensional (3D) cellulose scaffolds that can support mammalian 3D cell culture	246:330	(BF) immature endosperm was decellularized to produce three dimensional (3D) cellulose scaffolds that can support mammalian 3D cell culture.					
34420749	10	37	used	used	1267:1270	arg2	material					1302:1309	an alternative scaffolding material	1275:1309	an alternative scaffolding material in regenerative medicine	1275:1334	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	10	37	used	used	1267:1270	arg2	scaffolds					1248:1256	plant derived cellulosic scaffolds	1223:1256	plant derived cellulosic scaffolds	1223:1256	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	5	38	theme	complex	518:524	arg1	concoction					526:535	a complex concoction	516:535	a complex concoction of polysaccharides with intrinsic phyto-ingredients which provide excellent pharmacological properties	516:638	The results demonstrated that the BF tissue represented a complex concoction of polysaccharides with intrinsic phyto-ingredients which provide excellent pharmacological properties.					
34420749	8	39	from	strength	1027:1034	arg1	group					1046:1050	CS/CHI group	1039:1050	CS/CHI group	1039:1050	The physiochemical characterization showed reduced biodegradation, improved thermal stability and enhanced compressive strength in CS/CHI group.					
34420749	3	40	theme	3D	316:317	arg1	culture					324:330	mammalian 3D cell culture	306:330	mammalian 3D cell culture	306:330	(BF) immature endosperm was decellularized to produce three dimensional (3D) cellulose scaffolds that can support mammalian 3D cell culture.					
34420749	7	41	theme	comparative	783:793	arg1	investigation					795:807	The comparative investigation	779:807	The comparative investigation on both scaffolds	779:825	The comparative investigation on both scaffolds exhibited adequate swelling with controlled porosity and pore-size distribution.					
34420749	8	42	theme	compressive	1015:1025	arg1	strength					1027:1034	compressive strength	1015:1034	compressive strength in CS/CHI group	1015:1050	The physiochemical characterization showed reduced biodegradation, improved thermal stability and enhanced compressive strength in CS/CHI group.					
34420749	1	43	theme	tissue	115:120	arg1	applications					134:145	tissue engineering applications	115:145	tissue engineering applications	115:145	as extracellular matrix for tissue engineering applications.					
34420749	10	44	dep	derived	1229:1235	arg1	plant					1223:1227	plant	1223:1227	plant	1223:1227	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	5	45	theme	polysaccharides	540:554	arg1	concoction					526:535	a complex concoction	516:535	a complex concoction of polysaccharides with intrinsic phyto-ingredients which provide excellent pharmacological properties	516:638	The results demonstrated that the BF tissue represented a complex concoction of polysaccharides with intrinsic phyto-ingredients which provide excellent pharmacological properties.					
34420749	1	46	theme	engineering	122:132	arg1	applications					134:145	tissue engineering applications	115:145	tissue engineering applications	115:145	as extracellular matrix for tissue engineering applications.					
34420749	9	47	theme	fibroblasts	1121:1131	arg1	adhesion					1091:1098	favorable adhesion	1081:1098	favorable adhesion	1081:1098	Biological studies reported favorable adhesion and proliferation of fibroblasts with evident cellular penetration and colonization on the both scaffolds.					
34420749	9	47	theme	fibroblasts	1121:1131	arg1	proliferation					1104:1116	proliferation	1104:1116	proliferation	1104:1116	Biological studies reported favorable adhesion and proliferation of fibroblasts with evident cellular penetration and colonization on the both scaffolds.					
34420749	3	48	theme	cellulose	269:277	arg1	scaffolds					279:287	three dimensional (3D) cellulose scaffolds	246:287	three dimensional (3D) cellulose scaffolds that can support mammalian 3D cell culture	246:330	(BF) immature endosperm was decellularized to produce three dimensional (3D) cellulose scaffolds that can support mammalian 3D cell culture.					
34420749	5	49	theme	BF	494:495	arg1	tissue					497:502	the BF tissue	490:502	the BF tissue	490:502	The results demonstrated that the BF tissue represented a complex concoction of polysaccharides with intrinsic phyto-ingredients which provide excellent pharmacological properties.					
34420749	2	50	dep	Linn	185:188	arg1	study					156:160	this study	151:160	this study	151:160	In this study, Borassus flabellifer (Linn.)					
34420749	3	51	theme	mammalian	306:314	arg1	culture					324:330	mammalian 3D cell culture	306:330	mammalian 3D cell culture	306:330	(BF) immature endosperm was decellularized to produce three dimensional (3D) cellulose scaffolds that can support mammalian 3D cell culture.					
34420749	4	52	theme	nutritive	394:402	arg1	profile					404:410	nutritive profile	394:410	nutritive profile	394:410	To this regard, we first evaluated the chemical composition, nutritive profile and pharmacological activities of BF endosperm.					
34420749	6	53	theme	CS/CHI	753:758	arg1	scaffolds					768:776	cellulose-chitosan (CS/CHI) hybrid scaffolds	733:776	cellulose-chitosan (CS/CHI) hybrid scaffolds	733:776	Furthermore cellulosic scaffolds (CS) obtained from BF was treated with chitosan to produce cellulose-chitosan (CS/CHI) hybrid scaffolds.					
34420749	9	54	theme	evident	1138:1144	arg1	penetration					1155:1165	evident cellular penetration	1138:1165	evident cellular penetration	1138:1165	Biological studies reported favorable adhesion and proliferation of fibroblasts with evident cellular penetration and colonization on the both scaffolds.					
34420749	10	55	theme	alternative	1278:1288	arg1	scaffolds					1248:1256	plant derived cellulosic scaffolds	1223:1256	plant derived cellulosic scaffolds	1223:1256	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	10	55	theme	alternative	1278:1288	arg1	material					1302:1309	an alternative scaffolding material	1275:1309	an alternative scaffolding material in regenerative medicine	1275:1334	Taken together, plant derived cellulosic scaffolds could be used as an alternative scaffolding material in regenerative medicine.					
34420749	3	56	dep	dimensional	252:262	arg1	3D					265:266	3D	265:266	3D	265:266	(BF) immature endosperm was decellularized to produce three dimensional (3D) cellulose scaffolds that can support mammalian 3D cell culture.					
34420749	9	57	theme	cellular	1146:1153	arg1	penetration					1155:1165	evident cellular penetration	1138:1165	evident cellular penetration	1138:1165	Biological studies reported favorable adhesion and proliferation of fibroblasts with evident cellular penetration and colonization on the both scaffolds.					
33077860	0	0	theme	lead	105:108	arg1	stress					114:119	lead ion stress	105:119	lead ion stress	105:119	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	0	1	from	characteristics	14:28	arg1	removal					145:151	Pb removal	142:151	Pb removal	142:151	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	2	2	theme	main	368:371	arg1	composition					373:383	the main composition	364:383	the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS)	364:433	Polysaccharides was found to be the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS).					
33077860	2	2	theme	main	368:371	arg1	Polysaccharides					332:346	Polysaccharides	332:346	Polysaccharides	332:346	Polysaccharides was found to be the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS).					
33077860	4	3	from	%	658:658	arg1	group					663:667	group	663:667	group	663:667	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass gradually decreased from 91.66 to 61.27% in group with 50 mg/L of initial Pb2+, but kept at about 35% or 25% in groups with higher Pb2+.					
33077860	1	4	theme	Pb2+	269:272	arg1	stress					274:279	Pb2+ stress	269:279	Pb2+ stress	269:279	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	7	5	theme	precipitation	1213:1225	arg1	form					1202:1205	the form	1198:1205	the form of Pb precipitation	1198:1225	SEM-EDS analysis demonstrated that part of Pb immobilized in BEPS was in the form of Pb precipitation.					
33077860	1	6	from	distribution	158:169	arg1	removal					305:311	Pb removal	302:311	Pb removal	302:311	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	0	7	from	Distribution	0:11	arg1	removal					145:151	Pb removal	142:151	Pb removal	142:151	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	5	8	theme	concentration	903:915	arg1	removal					886:892	the removal	882:892	the removal of lower concentration of Pb2+	882:923	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	5	9	theme	BEPS	844:847	arg1	role					836:839	the role	832:839	the role of BEPS	832:847	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	5	9	theme	BEPS	844:847	arg1	important					869:877	important	869:877	important	869:877	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	0	10	theme	ion	110:112	arg1	stress					114:119	lead ion stress	105:119	lead ion stress	105:119	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	5	11	theme	removal	957:963	arg1	period					944:949	the initial period	932:949	the initial period of Pb removal	932:963	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	6	12	theme	adsorption	994:1003	arg1	experiment					1005:1014	adsorption experiment	994:1014	adsorption experiment	994:1014	With FTIR analysis and Pb2+ adsorption experiment, more effective functional groups and better Pb2+ adsorption capacity was demonstrated in BEPS than in SEPS.					
33077860	3	13	theme	Pb2+	510:513	arg1	concentration					515:527	the increased Pb2+ concentration	496:527	the increased Pb2+ concentration	496:527	More polysaccharides and protein in BEPS were detected with the increased Pb2+ concentration.					
33077860	2	14	theme	soluble	393:399	arg1	EPS					401:403	soluble EPS	393:403	soluble EPS (SEPS)	393:410	Polysaccharides was found to be the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS).					
33077860	2	14	theme	soluble	393:399	arg1	SEPS					406:409	SEPS	406:409	SEPS	406:409	Polysaccharides was found to be the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS).					
33077860	5	15	from	period	944:949	arg1	important					869:877	important	869:877	important	869:877	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	4	16	theme	amount	546:551	arg1	ratio					534:538	The ratio	530:538	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass	530:617	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass gradually decreased from 91.66 to 61.27% in group with 50 mg/L of initial Pb2+, but kept at about 35% or 25% in groups with higher Pb2+.					
33077860	3	17	from	protein	461:467	arg1	BEPS					472:475	BEPS	472:475	BEPS	472:475	More polysaccharides and protein in BEPS were detected with the increased Pb2+ concentration.					
33077860	6	18	theme	functional	1032:1041	arg1	groups					1043:1048	more effective functional groups	1017:1048	more effective functional groups	1017:1048	With FTIR analysis and Pb2+ adsorption experiment, more effective functional groups and better Pb2+ adsorption capacity was demonstrated in BEPS than in SEPS.					
33077860	5	19	from	important	869:877	arg1	period					944:949	the initial period	932:949	the initial period of Pb removal	932:963	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	5	19	from	important	869:877	arg1	removal					886:892	the removal	882:892	the removal of lower concentration of Pb2+	882:923	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	6	20	dep	experiment	1005:1014	arg1	capacity					1077:1084	adsorption capacity	1066:1084	adsorption capacity	1066:1084	With FTIR analysis and Pb2+ adsorption experiment, more effective functional groups and better Pb2+ adsorption capacity was demonstrated in BEPS than in SEPS.					
33077860	6	21	theme	effective	1022:1030	arg1	groups					1043:1048	more effective functional groups	1017:1048	more effective functional groups	1017:1048	With FTIR analysis and Pb2+ adsorption experiment, more effective functional groups and better Pb2+ adsorption capacity was demonstrated in BEPS than in SEPS.					
33077860	1	22	from	influence	289:297	arg1	removal					305:311	Pb removal	302:311	Pb removal	302:311	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	4	23	theme	Pb2+	693:696	arg1	50 mg/L					674:680	50 mg/L	674:680	50 mg/L of initial Pb2+	674:696	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass gradually decreased from 91.66 to 61.27% in group with 50 mg/L of initial Pb2+, but kept at about 35% or 25% in groups with higher Pb2+.					
33077860	8	24	from	weight	1252:1257	arg1	SEPS					1262:1265	SEPS	1262:1265	SEPS	1262:1265	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	8	24	from	weight	1252:1257	arg1	polysaccharides					1276:1290	more polysaccharides	1271:1290	more polysaccharides	1271:1290	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	1	25	theme	extracellular	191:203	arg1	EPS					227:229	EPS	227:229	EPS	227:229	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	1	25	theme	extracellular	191:203	arg1	substances					215:224	extracellular polymeric substances	191:224	extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress	191:279	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	5	26	from	removal	886:892	arg1	role					836:839	the role	832:839	the role of BEPS	832:847	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	5	26	from	removal	886:892	arg1	important					869:877	important	869:877	important	869:877	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	4	27	theme	initial	685:691	arg1	Pb2+					693:696	initial Pb2+	685:696	initial Pb2+	685:696	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass gradually decreased from 91.66 to 61.27% in group with 50 mg/L of initial Pb2+, but kept at about 35% or 25% in groups with higher Pb2+.					
33077860	8	28	theme	more	1271:1274	arg1	polysaccharides					1276:1290	more polysaccharides	1271:1290	more polysaccharides	1271:1290	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	1	29	theme	polymeric	205:213	arg1	EPS					227:229	EPS	227:229	EPS	227:229	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	1	29	theme	polymeric	205:213	arg1	substances					215:224	extracellular polymeric substances	191:224	extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress	191:279	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	2	30	from	composition	373:383	arg1	EPS					401:403	soluble EPS	393:403	soluble EPS (SEPS)	393:410	Polysaccharides was found to be the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS).					
33077860	2	30	from	composition	373:383	arg1	BEPS					429:432	BEPS	429:432	BEPS	429:432	Polysaccharides was found to be the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS).					
33077860	2	30	from	composition	373:383	arg1	EPS					424:426	bounded EPS	416:426	bounded EPS (BEPS)	416:433	Polysaccharides was found to be the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS).					
33077860	2	30	from	composition	373:383	arg1	SEPS					406:409	SEPS	406:409	SEPS	406:409	Polysaccharides was found to be the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS).					
33077860	8	31	theme	molecular	1242:1250	arg1	beneficial					1314:1323	beneficial	1314:1323	beneficial	1314:1323	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	8	31	theme	molecular	1242:1250	arg1	weight					1252:1257	The increased molecular weight	1228:1257	The increased molecular weight in SEPS and more polysaccharides in BEPS	1228:1298	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	1	32	theme	substances	215:224	arg1	influence					289:297	the influence	285:297	the influence on Pb removal	285:311	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	1	32	theme	substances	215:224	arg1	distribution					158:169	The distribution	154:169	The distribution	154:169	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	1	32	theme	substances	215:224	arg1	characteristics					172:186	characteristics	172:186	characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress	172:279	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	7	33	theme	Pb	1168:1169	arg1	part					1160:1163	part	1160:1163	part of Pb immobilized in BEPS	1160:1189	SEM-EDS analysis demonstrated that part of Pb immobilized in BEPS was in the form of Pb precipitation.					
33077860	1	34	theme	Pb	302:303	arg1	removal					305:311	Pb removal	302:311	Pb removal	302:311	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	4	35	dep	61.27	653:657	arg1	to					650:651	to	650:651	to	650:651	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass gradually decreased from 91.66 to 61.27% in group with 50 mg/L of initial Pb2+, but kept at about 35% or 25% in groups with higher Pb2+.					
33077860	5	36	theme	initial	936:942	arg1	period					944:949	the initial period	932:949	the initial period of Pb removal	932:963	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	8	37	theme	increased	1232:1240	arg1	beneficial					1314:1323	beneficial	1314:1323	beneficial	1314:1323	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	8	37	theme	increased	1232:1240	arg1	weight					1252:1257	The increased molecular weight	1228:1257	The increased molecular weight in SEPS and more polysaccharides in BEPS	1228:1298	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	8	38	from	polysaccharides	1276:1290	arg1	BEPS					1295:1298	BEPS	1295:1298	BEPS	1295:1298	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	6	39	theme	FTIR	971:974	arg1	analysis					976:983	FTIR analysis	971:983	FTIR analysis	971:983	With FTIR analysis and Pb2+ adsorption experiment, more effective functional groups and better Pb2+ adsorption capacity was demonstrated in BEPS than in SEPS.					
33077860	0	40	theme	polymeric	47:55	arg1	substances					57:66	extracellular polymeric substances	33:66	extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress	33:119	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	4	41	theme	Pb	543:544	arg1	amount					546:551	Pb amount	543:551	Pb amount distributed in BEPS	543:571	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass gradually decreased from 91.66 to 61.27% in group with 50 mg/L of initial Pb2+, but kept at about 35% or 25% in groups with higher Pb2+.					
33077860	8	42	from	SEPS	1262:1265	arg1	BEPS					1295:1298	BEPS	1295:1298	BEPS	1295:1298	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	0	43	theme	extracellular	33:45	arg1	substances					57:66	extracellular polymeric substances	33:66	extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress	33:119	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	0	44	theme	Pb	142:143	arg1	removal					145:151	Pb removal	142:151	Pb removal	142:151	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	4	45	with	groups	731:736	arg1	Pb2+					750:753	higher Pb2+	743:753	higher Pb2+	743:753	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass gradually decreased from 91.66 to 61.27% in group with 50 mg/L of initial Pb2+, but kept at about 35% or 25% in groups with higher Pb2+.					
33077860	3	46	theme	More	436:439	arg1	polysaccharides					441:455	More polysaccharides	436:455	More polysaccharides	436:455	More polysaccharides and protein in BEPS were detected with the increased Pb2+ concentration.					
33077860	5	47	theme	Pb	954:955	arg1	removal					957:963	Pb removal	954:963	Pb removal	954:963	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	4	48	theme	higher	743:748	arg1	Pb2+					750:753	higher Pb2+	743:753	higher Pb2+	743:753	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass gradually decreased from 91.66 to 61.27% in group with 50 mg/L of initial Pb2+, but kept at about 35% or 25% in groups with higher Pb2+.					
33077860	5	49	theme	lead	806:809	arg1	process					819:825	the lead removal process	802:825	the lead removal process	802:825	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	0	50	theme	substances	57:66	arg1	characteristics					14:28	characteristics	14:28	characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress	14:119	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	0	50	theme	substances	57:66	arg1	Distribution					0:11	Distribution	0:11	Distribution	0:11	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	0	50	theme	substances	57:66	arg1	influence					129:137	the influence	125:137	the influence on Pb removal	125:151	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	5	51	theme	Pb2+	920:923	arg1	concentration					903:915	lower concentration	897:915	lower concentration of Pb2+	897:923	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	3	52	from	polysaccharides	441:455	arg1	BEPS					472:475	BEPS	472:475	BEPS	472:475	More polysaccharides and protein in BEPS were detected with the increased Pb2+ concentration.					
33077860	5	53	theme	removal	811:817	arg1	process					819:825	the lead removal process	802:825	the lead removal process	802:825	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	7	54	theme	Pb	1210:1211	arg1	precipitation					1213:1225	Pb precipitation	1210:1225	Pb precipitation	1210:1225	SEM-EDS analysis demonstrated that part of Pb immobilized in BEPS was in the form of Pb precipitation.					
33077860	5	55	theme	lower	897:901	arg1	concentration					903:915	lower concentration	897:915	lower concentration of Pb2+	897:923	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	4	56	theme	total	580:584	arg1	Pb					586:587	the total Pb	576:587	the total Pb removed by the fungal biomass	576:617	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass gradually decreased from 91.66 to 61.27% in group with 50 mg/L of initial Pb2+, but kept at about 35% or 25% in groups with higher Pb2+.					
33077860	8	57	theme	Pb	1345:1346	arg1	precipitation					1348:1360	Pb precipitation	1345:1360	Pb precipitation	1345:1360	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	6	58	theme	better	1054:1059	arg1	Pb2+					1061:1064	better Pb2+	1054:1064	better Pb2+	1054:1064	With FTIR analysis and Pb2+ adsorption experiment, more effective functional groups and better Pb2+ adsorption capacity was demonstrated in BEPS than in SEPS.					
33077860	8	59	theme	precipitation	1348:1360	arg1	adhesion					1333:1340	the adhesion	1329:1340	the adhesion of Pb precipitation	1329:1360	The increased molecular weight in SEPS and more polysaccharides in BEPS were probably beneficial for the adhesion of Pb precipitation.					
33077860	7	60	theme	SEM-EDS	1125:1131	arg1	analysis					1133:1140	SEM-EDS analysis	1125:1140	SEM-EDS analysis	1125:1140	SEM-EDS analysis demonstrated that part of Pb immobilized in BEPS was in the form of Pb precipitation.					
33077860	1	61	theme	Phanerochaete	235:247	arg1	chrysosporium					249:261	Phanerochaete chrysosporium	235:261	Phanerochaete chrysosporium under Pb2+ stress	235:279	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	0	62	theme	chrysosporium	85:97	arg1	substances					57:66	extracellular polymeric substances	33:66	extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress	33:119	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	4	63	theme	fungal	604:609	arg1	biomass					611:617	the fungal biomass	600:617	the fungal biomass	600:617	The ratio of Pb amount distributed in BEPS to the total Pb removed by the fungal biomass gradually decreased from 91.66 to 61.27% in group with 50 mg/L of initial Pb2+, but kept at about 35% or 25% in groups with higher Pb2+.					
33077860	6	64	theme	adsorption	1066:1075	arg1	capacity					1077:1084	adsorption capacity	1066:1084	adsorption capacity	1066:1084	With FTIR analysis and Pb2+ adsorption experiment, more effective functional groups and better Pb2+ adsorption capacity was demonstrated in BEPS than in SEPS.					
33077860	2	65	theme	bounded	416:422	arg1	BEPS					429:432	BEPS	429:432	BEPS	429:432	Polysaccharides was found to be the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS).					
33077860	2	65	theme	bounded	416:422	arg1	EPS					424:426	bounded EPS	416:426	bounded EPS (BEPS)	416:433	Polysaccharides was found to be the main composition in both soluble EPS (SEPS) and bounded EPS (BEPS).					
33077860	5	66	theme	certain	786:792	arg1	role					794:797	a certain role	784:797	a certain role	784:797	It implies that BEPS played a certain role in the lead removal process, and the role of BEPS was relatively more important in the removal of lower concentration of Pb2+ and in the initial period of Pb removal.					
33077860	6	67	from	BEPS	1106:1109	arg1	SEPS					1119:1122	SEPS	1119:1122	SEPS	1119:1122	With FTIR analysis and Pb2+ adsorption experiment, more effective functional groups and better Pb2+ adsorption capacity was demonstrated in BEPS than in SEPS.					
33077860	1	68	theme	chrysosporium	249:261	arg1	EPS					227:229	EPS	227:229	EPS	227:229	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	1	68	theme	chrysosporium	249:261	arg1	substances					215:224	extracellular polymeric substances	191:224	extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress	191:279	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	0	69	theme	Phanerochaete	71:83	arg1	chrysosporium					85:97	Phanerochaete chrysosporium	71:97	Phanerochaete chrysosporium under lead ion stress	71:119	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33077860	1	70	from	characteristics	172:186	arg1	removal					305:311	Pb removal	302:311	Pb removal	302:311	The distribution, characteristics of extracellular polymeric substances (EPS) of Phanerochaete chrysosporium under Pb2+ stress and the influence on Pb removal were investigated.					
33077860	3	71	theme	increased	500:508	arg1	concentration					515:527	the increased Pb2+ concentration	496:527	the increased Pb2+ concentration	496:527	More polysaccharides and protein in BEPS were detected with the increased Pb2+ concentration.					
33077860	0	72	from	influence	129:137	arg1	removal					145:151	Pb removal	142:151	Pb removal	142:151	Distribution, characteristics of extracellular polymeric substances of Phanerochaete chrysosporium under lead ion stress and the influence on Pb removal.					
33667082	0	0	theme	Mass	97:100	arg1	Spectrometry					102:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry.					
33667082	4	1	theme	Novel	507:511	arg1	strategies					523:532	Novel synthetic strategies	507:532	Novel synthetic strategies	507:532	Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization.					
33667082	5	2	theme	cyclotron	823:831	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	2	theme	cyclotron	823:831	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	2	theme	cyclotron	823:831	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	6	3	theme	bond	1135:1138	arg1	cleavage					1140:1147	glycosidic bond cleavage	1124:1147	glycosidic bond cleavage (or cross-ring cleavage)	1124:1172	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	6	3	theme	bond	1135:1138	arg1	cleavage					1164:1171	cross-ring cleavage	1153:1171	cross-ring cleavage	1153:1171	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	5	4	theme	Fourier	801:807	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	4	theme	Fourier	801:807	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	4	theme	Fourier	801:807	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	0	5	theme	MALDI	91:95	arg1	Spectrometry					102:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry.					
33667082	6	6	theme	Synthetic	956:964	arg1	carbohydrates					966:978	Synthetic carbohydrates	956:978	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers	956:1007	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	5	7	theme	polysaccharides	939:953	arg1	analysis					906:913	the analysis	902:913	the analysis of synthetic oligo- and polysaccharides	902:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	6	8	dep	analyzed	1019:1026	arg1	mass					1014:1017	mass	1014:1017	mass	1014:1017	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	5	9	dep	spectrometry	857:868	arg1	transform					809:817	transform	809:817	transform	809:817	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	10	theme	MALDI	794:798	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	10	theme	MALDI	794:798	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	10	theme	MALDI	794:798	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	6	11	theme	structures	1315:1324	arg1	corroboration					1264:1276	the corroboration	1260:1276	the corroboration of the carbohydrate compositions and structures	1260:1324	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	6	12	dep	carbohydrates	966:978	arg1	100-					990:993	100-	990:993	100-	990:993	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	6	12	dep	carbohydrates	966:978	arg1	151-mers					1000:1007	151-mers	1000:1007	151-mers	1000:1007	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	6	12	dep	carbohydrates	966:978	arg1	64-					985:987	64-	985:987	64-	985:987	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	6	12	dep	carbohydrates	966:978	arg1	16-					980:982	16-	980:982	16-	980:982	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	3	13	with	compounds	409:417	arg1	structures					437:446	well-defined structures	424:446	well-defined structures that can be obtained from chemical or enzymatic syntheses	424:504	However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses.					
33667082	6	14	theme	ions	1104:1107	arg1	Detection					1082:1090	Detection	1082:1090	Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage)	1082:1172	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	1	15	theme	abundant	178:185	arg1	oligo-					139:144	oligo-	139:144	oligo-	139:144	Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes.					
33667082	1	15	theme	abundant	178:185	arg1	Carbohydrates					116:128	Carbohydrates	116:128	Carbohydrates	116:128	Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes.					
33667082	1	15	theme	abundant	178:185	arg1	biopolymers					187:197	highly abundant biopolymers	171:197	highly abundant biopolymers that are involved in numerous processes	171:237	Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes.					
33667082	1	15	theme	abundant	178:185	arg1	polysaccharides					150:164	polysaccharides	150:164	polysaccharides	150:164	Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes.					
33667082	0	16	theme	Wide	54:57	arg1	Spectrometry					102:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry.					
33667082	2	17	attach	isolated	323:330	arg2	material					306:313	a material	304:313	a material that is isolated from complex natural sources	304:359	The study of their structure and functions is commonly based on a material that is isolated from complex natural sources.					
33667082	2	17	attach	isolated	323:330	arg1	sources					353:359	complex natural sources	337:359	complex natural sources	337:359	The study of their structure and functions is commonly based on a material that is isolated from complex natural sources.					
33667082	6	18	theme	compositions	1298:1309	arg1	corroboration					1264:1276	the corroboration	1260:1276	the corroboration of the carbohydrate compositions and structures	1260:1324	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	6	19	theme	monosaccharide	1202:1215	arg1	content					1217:1223	the monosaccharide content	1198:1223	the monosaccharide content	1198:1223	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	3	20	theme	precise	378:384	arg1	analysis					386:393	a more precise analysis	371:393	a more precise analysis	371:393	However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses.					
33667082	5	21	theme	resonance	833:841	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	21	theme	resonance	833:841	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	21	theme	resonance	833:841	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	6	22	theme	carbohydrate	1285:1296	arg1	compositions					1298:1309	the carbohydrate compositions	1281:1309	the carbohydrate compositions	1281:1309	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	3	23	theme	well-defined	424:435	arg1	structures					437:446	well-defined structures	424:446	well-defined structures that can be obtained from chemical or enzymatic syntheses	424:504	However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses.					
33667082	6	24	theme	decay	1065:1069	arg1	FT-ICR					1071:1076	MALDI in-source decay FT-ICR	1049:1076	MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures	1049:1324	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	4	25	theme	monodisperse	577:588	arg1	polysaccharides					590:604	larger monodisperse polysaccharides	570:604	larger monodisperse polysaccharides	570:604	Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization.					
33667082	6	26	theme	fragment	1095:1102	arg1	ions					1104:1107	fragment ions	1095:1107	fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage)	1095:1172	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	0	27	theme	Monodisperse	22:33	arg1	Polysaccharides					35:49	Synthetic Monodisperse Polysaccharides	12:49	Synthetic Monodisperse Polysaccharides	12:49	Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry.					
33667082	4	28	theme	larger	570:575	arg1	polysaccharides					590:604	larger monodisperse polysaccharides	570:604	larger monodisperse polysaccharides	570:604	Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization.					
33667082	5	29	theme	ultrahigh-resolution	728:747	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	29	theme	ultrahigh-resolution	728:747	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	29	theme	ultrahigh-resolution	728:747	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	0	30	theme	Synthetic	12:20	arg1	Polysaccharides					35:49	Synthetic Monodisperse Polysaccharides	12:49	Synthetic Monodisperse Polysaccharides	12:49	Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry.					
33667082	5	31	theme	matrix-assisted	749:763	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	31	theme	matrix-assisted	749:763	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	31	theme	matrix-assisted	749:763	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	6	32	theme	glycosidic	1124:1133	arg1	cleavage					1140:1147	glycosidic bond cleavage	1124:1147	glycosidic bond cleavage (or cross-ring cleavage)	1124:1172	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	6	32	theme	glycosidic	1124:1133	arg1	cleavage					1164:1171	cross-ring cleavage	1153:1171	cross-ring cleavage	1153:1171	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	6	33	theme	type	1241:1244	arg1	information					1183:1193	information	1183:1193	information of the monosaccharide content and the linkage type	1183:1244	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	3	34	theme	chemical	474:481	arg1	syntheses					496:504	chemical or enzymatic syntheses	474:504	chemical or enzymatic syntheses	474:504	However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses.					
33667082	4	35	theme	polysaccharides	590:604	arg1	accessibility					553:565	the accessibility	549:565	the accessibility of larger monodisperse polysaccharides	549:604	Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization.					
33667082	5	36	theme	laser	765:769	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	36	theme	laser	765:769	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	36	theme	laser	765:769	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	37	theme	powerful	880:887	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	37	theme	powerful	880:887	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	1	38	theme	numerous	220:227	arg1	processes					229:237	numerous processes	220:237	numerous processes	220:237	Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes.					
33667082	0	39	theme	Polysaccharides	35:49	arg1	Analysis					0:7	Analysis	0:7	Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry.	0:114	Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry.					
33667082	5	40	theme	desorption/ionization	771:791	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	40	theme	desorption/ionization	771:791	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	40	theme	desorption/ionization	771:791	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	4	41	theme	molecular	667:675	arg1	characterization					677:692	their molecular characterization	661:692	their molecular characterization	661:692	Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization.					
33667082	0	42	theme	Mass	59:62	arg1	Spectrometry					102:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry.					
33667082	5	43	theme	mass	852:855	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	43	theme	mass	852:855	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	43	theme	mass	852:855	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	44	theme	ion	819:821	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	44	theme	ion	819:821	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	44	theme	ion	819:821	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	2	45	theme	natural	345:351	arg1	sources					353:359	complex natural sources	337:359	complex natural sources	337:359	The study of their structure and functions is commonly based on a material that is isolated from complex natural sources.					
33667082	3	46	theme	pure	404:407	arg1	compounds					409:417	pure compounds	404:417	pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses	404:504	However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses.					
33667082	5	47	theme	wide	712:715	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	47	theme	wide	712:715	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	47	theme	wide	712:715	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	2	48	theme	complex	337:343	arg1	sources					353:359	complex natural sources	337:359	complex natural sources	337:359	The study of their structure and functions is commonly based on a material that is isolated from complex natural sources.					
33667082	6	49	theme	linkage	1233:1239	arg1	type					1241:1244	the linkage type	1229:1244	the linkage type	1229:1244	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	5	50	theme	mass	717:720	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	50	theme	mass	717:720	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	50	theme	mass	717:720	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	2	51	theme	functions	273:281	arg1	study					244:248	The study	240:248	The study of their structure and functions	240:281	The study of their structure and functions is commonly based on a material that is isolated from complex natural sources.					
33667082	6	52	theme	cross-ring	1153:1162	arg1	cleavage					1140:1147	glycosidic bond cleavage	1124:1147	glycosidic bond cleavage (or cross-ring cleavage)	1124:1172	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	6	52	theme	cross-ring	1153:1162	arg1	cleavage					1164:1171	cross-ring cleavage	1153:1171	cross-ring cleavage	1153:1171	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	4	53	theme	analytical	633:642	arg1	methods					644:650	the analytical methods	629:650	the analytical methods used for their molecular characterization	629:692	Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization.					
33667082	5	54	theme	range	722:726	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	54	theme	range	722:726	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	54	theme	range	722:726	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	3	55	theme	enzymatic	486:494	arg1	syntheses					496:504	chemical or enzymatic syntheses	474:504	chemical or enzymatic syntheses	474:504	However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses.					
33667082	6	56	theme	in-source	1055:1063	arg1	FT-ICR					1071:1076	MALDI in-source decay FT-ICR	1049:1076	MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures	1049:1324	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	0	57	theme	Ultrahigh-Resolution	70:89	arg1	Spectrometry					102:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry.					
33667082	2	58	theme	structure	259:267	arg1	study					244:248	The study	240:248	The study of their structure and functions	240:281	The study of their structure and functions is commonly based on a material that is isolated from complex natural sources.					
33667082	5	59	theme	synthetic	918:926	arg1	oligo-					928:933	synthetic oligo-	918:933	synthetic oligo-	918:933	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	6	60	theme	content	1217:1223	arg1	information					1183:1193	information	1183:1193	information of the monosaccharide content and the linkage type	1183:1244	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	6	61	theme	MALDI	1049:1053	arg1	FT-ICR					1071:1076	MALDI in-source decay FT-ICR	1049:1076	MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures	1049:1324	Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.					
33667082	0	62	theme	Range	64:68	arg1	Spectrometry					102:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry	54:113	Analysis of Synthetic Monodisperse Polysaccharides by Wide Mass Range Ultrahigh-Resolution MALDI Mass Spectrometry.					
33667082	4	63	theme	synthetic	513:521	arg1	strategies					523:532	Novel synthetic strategies	507:532	Novel synthetic strategies	507:532	Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization.					
33667082	5	64	theme	FT-ICR	844:849	arg1	platform					889:896	a powerful platform	878:896	a powerful platform for the analysis of synthetic oligo- and polysaccharides	878:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	64	theme	FT-ICR	844:849	arg1	MS					871:872	MS	871:872	MS	871:872	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	64	theme	FT-ICR	844:849	arg1	spectrometry					857:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry	712:868	wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS)	712:873	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
33667082	5	65	theme	oligo-	928:933	arg1	analysis					906:913	the analysis	902:913	the analysis of synthetic oligo- and polysaccharides	902:953	Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides.					
34957619	6	0	theme	N-glycomic	739:748	arg1	profiles					750:757	the N-glycomic profiles	735:757	the N-glycomic profiles	735:757	MALDI-TOF MS was used to characterize the N-glycomic profiles.					
34957619	12	1	theme	molecules	1714:1722	arg1	α2,6-sialylation					1680:1695	ST6GAL1-mediated α2,6-sialylation	1663:1695	ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules	1663:1722	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	5	2	dep	MATERIALS	599:607	arg1	tissues					645:651	Uterine and endometrium tissues	621:651	Uterine and endometrium tissues	621:651	MATERIALS AND METHODS Uterine and endometrium tissues during implantation were collected in pigs.					
34957619	12	3	theme	uterine	1621:1627	arg1	closure					1635:1641	uterine lumen closure	1621:1641	uterine lumen closure	1621:1641	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	3	4	theme	endometrial	336:346	arg1	extension					353:361	endometrial fold extension	336:361	endometrial fold extension	336:361	Our previous study has reported that endometrial fold extension can lead to uterine lumen closure in pigs.					
34957619	4	5	theme	molecular	520:528	arg1	basis					530:534	the molecular basis	516:534	the molecular basis of the endometrial fold extension	516:568	The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.					
34957619	9	6	theme	α2,6-linked	1221:1231	arg1	acid					1240:1243	the α2,6-linked sialic acid	1217:1243	the α2,6-linked sialic acid	1217:1243	For the first time, we identified a large number of N-glycan compositions from endometrium during implantation using MALDI-TOF MS. Notably, the α2,6-linked sialic acid and ST6GAL1 were highly expressed in uterine LE when the endometrial folds extended greatly.					
34957619	11	7	theme	uterine	1564:1570	arg1	LE					1572:1573	uterine LE	1564:1573	uterine LE	1564:1573	Finally, we found that ST6GAL1-mediated α2,6-sialylation of E-cadherin may participate in collective migration of uterine LE.					
34957619	9	8	theme	sialic	1233:1238	arg1	acid					1240:1243	the α2,6-linked sialic acid	1217:1243	the α2,6-linked sialic acid	1217:1243	For the first time, we identified a large number of N-glycan compositions from endometrium during implantation using MALDI-TOF MS. Notably, the α2,6-linked sialic acid and ST6GAL1 were highly expressed in uterine LE when the endometrial folds extended greatly.					
34957619	8	9	dep	RESULTS	963:969	arg1	observed					974:981	observed	974:981	observed that uterine luminal epithelium (LE) migrated collectively during endometrial fold extension	974:1074	RESULTS We observed that uterine luminal epithelium (LE) migrated collectively during endometrial fold extension.					
34957619	9	10	link	α2,6-linked	1221:1231	arg1	acid					1240:1243	the α2,6-linked sialic acid	1217:1243	the α2,6-linked sialic acid	1217:1243	For the first time, we identified a large number of N-glycan compositions from endometrium during implantation using MALDI-TOF MS. Notably, the α2,6-linked sialic acid and ST6GAL1 were highly expressed in uterine LE when the endometrial folds extended greatly.					
34957619	3	11	theme	uterine	375:381	arg1	closure					389:395	uterine lumen closure	375:395	uterine lumen closure in pigs	375:403	Our previous study has reported that endometrial fold extension can lead to uterine lumen closure in pigs.					
34957619	4	12	theme	uterine	476:482	arg1	closure					490:496	the uterine lumen closure	472:496	the uterine lumen closure	472:496	The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.					
34957619	6	13	used	used	714:717	arg2	MS					707:708	MALDI-TOF MS	697:708	MALDI-TOF MS	697:708	MALDI-TOF MS was used to characterize the N-glycomic profiles.					
34957619	5	14	theme	Uterine	621:627	arg1	tissues					645:651	Uterine and endometrium tissues	621:651	Uterine and endometrium tissues	621:651	MATERIALS AND METHODS Uterine and endometrium tissues during implantation were collected in pigs.					
34957619	11	15	theme	collective	1540:1549	arg1	migration					1551:1559	collective migration	1540:1559	collective migration of uterine LE	1540:1573	Finally, we found that ST6GAL1-mediated α2,6-sialylation of E-cadherin may participate in collective migration of uterine LE.					
34957619	7	16	theme	cell	895:898	arg1	aggregation					900:910	cell aggregation	895:910	cell aggregation	895:910	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	9	17	dep	endometrial	1302:1312	arg1	folds					1314:1318	folds	1314:1318	folds	1314:1318	For the first time, we identified a large number of N-glycan compositions from endometrium during implantation using MALDI-TOF MS. Notably, the α2,6-linked sialic acid and ST6GAL1 were highly expressed in uterine LE when the endometrial folds extended greatly.					
34957619	3	18	from	closure	389:395	arg1	pigs					400:403	pigs	400:403	pigs	400:403	Our previous study has reported that endometrial fold extension can lead to uterine lumen closure in pigs.					
34957619	5	19	theme	endometrium	633:643	arg1	tissues					645:651	Uterine and endometrium tissues	621:651	Uterine and endometrium tissues	621:651	MATERIALS AND METHODS Uterine and endometrium tissues during implantation were collected in pigs.					
34957619	12	20	theme	LE	1817:1818	arg1	migration					1796:1804	collective migration	1785:1804	collective migration of uterine LE	1785:1818	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	7	21	theme	aggregation	900:910	arg1	assays					864:869	assays	864:869	assays	864:869	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	21	theme	aggregation	900:910	arg1	transfection					782:793	siRNA transfection	776:793	siRNA transfection	776:793	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	21	theme	aggregation	900:910	arg1	immumoprecipitation					821:839	lectin immumoprecipitation	814:839	lectin immumoprecipitation	814:839	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	21	theme	aggregation	900:910	arg1	spectrometry					847:858	mass spectrometry	842:858	mass spectrometry	842:858	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	21	theme	aggregation	900:910	arg1	Histochemistry					760:773	Histochemistry	760:773	Histochemistry	760:773	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	21	theme	aggregation	900:910	arg1	blotting					804:811	Western blotting	796:811	Western blotting	796:811	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	11	22	theme	ST6GAL1-mediated	1473:1488	arg1	α2,6-sialylation					1490:1505	ST6GAL1-mediated α2,6-sialylation	1473:1505	ST6GAL1-mediated α2,6-sialylation of E-cadherin	1473:1519	Finally, we found that ST6GAL1-mediated α2,6-sialylation of E-cadherin may participate in collective migration of uterine LE.					
34957619	4	23	theme	closure	490:496	arg1	mechanisms					458:467	molecular mechanisms	448:467	molecular mechanisms of the uterine lumen closure	448:496	The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.					
34957619	12	24	theme	collective	1785:1794	arg1	migration					1796:1804	collective migration	1785:1804	collective migration of uterine LE	1785:1818	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	8	25	theme	endometrial	1049:1059	arg1	extension					1066:1074	endometrial fold extension	1049:1074	endometrial fold extension	1049:1074	RESULTS We observed that uterine luminal epithelium (LE) migrated collectively during endometrial fold extension.					
34957619	12	26	dep	CONCLUSIONS	1576:1586	arg1	reveals					1598:1604	reveals	1598:1604	reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE	1598:1818	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	7	27	theme	healing	883:889	arg1	assays					864:869	assays	864:869	assays	864:869	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	27	theme	healing	883:889	arg1	transfection					782:793	siRNA transfection	776:793	siRNA transfection	776:793	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	27	theme	healing	883:889	arg1	immumoprecipitation					821:839	lectin immumoprecipitation	814:839	lectin immumoprecipitation	814:839	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	27	theme	healing	883:889	arg1	spectrometry					847:858	mass spectrometry	842:858	mass spectrometry	842:858	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	27	theme	healing	883:889	arg1	Histochemistry					760:773	Histochemistry	760:773	Histochemistry	760:773	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	27	theme	healing	883:889	arg1	blotting					804:811	Western blotting	796:811	Western blotting	796:811	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	7	28	theme	Western	796:802	arg1	blotting					804:811	Western blotting	796:811	Western blotting	796:811	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	4	29	from	implantation	577:588	arg1	pigs					593:596	pigs	593:596	pigs	593:596	The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.					
34957619	4	30	theme	lumen	484:488	arg1	closure					490:496	the uterine lumen closure	472:496	the uterine lumen closure	472:496	The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.					
34957619	7	31	theme	mass	842:845	arg1	spectrometry					847:858	mass spectrometry	842:858	mass spectrometry	842:858	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	1	32	theme	OBJECTIVES	104:113	arg1	cause					147:151	a major cause	139:151	a major cause of prenatal mortality	139:173	OBJECTIVES Implantation failure is a major cause of prenatal mortality.					
34957619	1	32	theme	OBJECTIVES	104:113	arg1	failure					128:134	OBJECTIVES Implantation failure	104:134	OBJECTIVES Implantation failure	104:134	OBJECTIVES Implantation failure is a major cause of prenatal mortality.					
34957619	12	33	gly	α2,6-sialylation	1680:1695	arg1	fold					1751:1754	endometrial fold extension	1739:1764	endometrial fold extension	1739:1764	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	12	33	gly	α2,6-sialylation	1680:1695	arg1	molecules					1714:1722	cell adhesion molecules	1700:1722	cell adhesion molecules	1700:1722	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	4	34	theme	extension	560:568	arg1	basis					530:534	the molecular basis	516:534	the molecular basis of the endometrial fold extension	516:568	The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.					
34957619	1	35	theme	Implantation	115:126	arg1	cause					147:151	a major cause	139:151	a major cause of prenatal mortality	139:173	OBJECTIVES Implantation failure is a major cause of prenatal mortality.					
34957619	1	35	theme	Implantation	115:126	arg1	failure					128:134	OBJECTIVES Implantation failure	104:134	OBJECTIVES Implantation failure	104:134	OBJECTIVES Implantation failure is a major cause of prenatal mortality.					
34957619	9	36	theme	N-glycan	1129:1136	arg1	compositions					1138:1149	N-glycan compositions	1129:1149	N-glycan compositions	1129:1149	For the first time, we identified a large number of N-glycan compositions from endometrium during implantation using MALDI-TOF MS. Notably, the α2,6-linked sialic acid and ST6GAL1 were highly expressed in uterine LE when the endometrial folds extended greatly.					
34957619	3	37	theme	lumen	383:387	arg1	closure					389:395	uterine lumen closure	375:395	uterine lumen closure in pigs	375:403	Our previous study has reported that endometrial fold extension can lead to uterine lumen closure in pigs.					
34957619	4	38	theme	fold	555:558	arg1	extension					560:568	the endometrial fold extension	539:568	the endometrial fold extension	539:568	The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.					
34957619	8	39	theme	fold	1061:1064	arg1	extension					1066:1074	endometrial fold extension	1049:1074	endometrial fold extension	1049:1074	RESULTS We observed that uterine luminal epithelium (LE) migrated collectively during endometrial fold extension.					
34957619	7	40	theme	wounding	874:881	arg1	healing					883:889	wounding healing	874:889	wounding healing	874:889	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	12	41	theme	endometrial	1739:1749	arg1	extension					1756:1764	endometrial fold extension	1739:1764	endometrial fold extension	1739:1764	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	4	42	theme	molecular	448:456	arg1	mechanisms					458:467	molecular mechanisms	448:467	molecular mechanisms of the uterine lumen closure	448:496	The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.					
34957619	6	43	theme	MALDI-TOF	697:705	arg1	MS					707:708	MALDI-TOF MS	697:708	MALDI-TOF MS	697:708	MALDI-TOF MS was used to characterize the N-glycomic profiles.					
34957619	10	44	theme	ST6GAL1-mediated	1364:1379	arg1	2,6-sialylation					1381:1395	ST6GAL1-mediated 2,6-sialylation	1364:1395	ST6GAL1-mediated 2,6-sialylation	1364:1395	Subsequently, the role of ST6GAL1-mediated 2,6-sialylation in collective epithelial migration was demonstrated.					
34957619	12	45	theme	lumen	1629:1633	arg1	closure					1635:1641	uterine lumen closure	1621:1641	uterine lumen closure	1621:1641	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	9	46	theme	large	1113:1117	arg1	number					1119:1124	a large number	1111:1124	a large number of N-glycan compositions	1111:1149	For the first time, we identified a large number of N-glycan compositions from endometrium during implantation using MALDI-TOF MS. Notably, the α2,6-linked sialic acid and ST6GAL1 were highly expressed in uterine LE when the endometrial folds extended greatly.					
34957619	9	47	theme	uterine	1282:1288	arg1	LE					1290:1291	uterine LE	1282:1291	uterine LE	1282:1291	For the first time, we identified a large number of N-glycan compositions from endometrium during implantation using MALDI-TOF MS. Notably, the α2,6-linked sialic acid and ST6GAL1 were highly expressed in uterine LE when the endometrial folds extended greatly.					
34957619	9	48	theme	first	1085:1089	arg1	time					1091:1094	the first time	1081:1094	the first time	1081:1094	For the first time, we identified a large number of N-glycan compositions from endometrium during implantation using MALDI-TOF MS. Notably, the α2,6-linked sialic acid and ST6GAL1 were highly expressed in uterine LE when the endometrial folds extended greatly.					
34957619	2	49	theme	lumen	188:192	arg1	closure					194:200	The uterine lumen closure	176:200	The uterine lumen closure	176:200	The uterine lumen closure contributes to embryo adhesion to the uterus, but its underlying mechanisms are largely unknown.					
34957619	0	50	theme	ST6GAL1-mediated	22:37	arg1	sialylation					39:49	ST6GAL1-mediated sialylation	22:49	ST6GAL1-mediated sialylation	22:49	Glycomics reveal that ST6GAL1-mediated sialylation regulates uterine lumen closure during implantation.					
34957619	10	51	theme	epithelial	1411:1420	arg1	migration					1422:1430	collective epithelial migration	1400:1430	collective epithelial migration	1400:1430	Subsequently, the role of ST6GAL1-mediated 2,6-sialylation in collective epithelial migration was demonstrated.					
34957619	0	52	theme	lumen	69:73	arg1	closure					75:81	uterine lumen closure	61:81	uterine lumen closure	61:81	Glycomics reveal that ST6GAL1-mediated sialylation regulates uterine lumen closure during implantation.					
34957619	7	53	theme	lectin	814:819	arg1	immumoprecipitation					821:839	lectin immumoprecipitation	814:839	lectin immumoprecipitation	814:839	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	2	54	theme	uterine	180:186	arg1	closure					194:200	The uterine lumen closure	176:200	The uterine lumen closure	176:200	The uterine lumen closure contributes to embryo adhesion to the uterus, but its underlying mechanisms are largely unknown.					
34957619	10	55	theme	collective	1400:1409	arg1	migration					1422:1430	collective epithelial migration	1400:1430	collective epithelial migration	1400:1430	Subsequently, the role of ST6GAL1-mediated 2,6-sialylation in collective epithelial migration was demonstrated.					
34957619	12	56	theme	adhesion	1705:1712	arg1	molecules					1714:1722	cell adhesion molecules	1700:1722	cell adhesion molecules	1700:1722	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	0	57	theme	uterine	61:67	arg1	closure					75:81	uterine lumen closure	61:81	uterine lumen closure	61:81	Glycomics reveal that ST6GAL1-mediated sialylation regulates uterine lumen closure during implantation.					
34957619	4	58	theme	endometrial	543:553	arg1	extension					560:568	the endometrial fold extension	539:568	the endometrial fold extension	539:568	The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.					
34957619	4	59	theme	study	428:432	arg1	objective					410:418	The objective	406:418	The objective of this study	406:432	The objective of this study was to reveal molecular mechanisms of the uterine lumen closure by characterizing the molecular basis of the endometrial fold extension during implantation in pigs.					
34957619	11	60	gly	α2,6-sialylation	1490:1505	arg1	E-cadherin					1510:1519	E-cadherin	1510:1519	E-cadherin	1510:1519	Finally, we found that ST6GAL1-mediated α2,6-sialylation of E-cadherin may participate in collective migration of uterine LE.					
34957619	1	61	theme	major	141:145	arg1	cause					147:151	a major cause	139:151	a major cause of prenatal mortality	139:173	OBJECTIVES Implantation failure is a major cause of prenatal mortality.					
34957619	1	61	theme	major	141:145	arg1	failure					128:134	OBJECTIVES Implantation failure	104:134	OBJECTIVES Implantation failure	104:134	OBJECTIVES Implantation failure is a major cause of prenatal mortality.					
34957619	12	62	theme	cell	1700:1703	arg1	molecules					1714:1722	cell adhesion molecules	1700:1722	cell adhesion molecules	1700:1722	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	3	63	theme	previous	303:310	arg1	study					312:316	Our previous study	299:316	Our previous study	299:316	Our previous study has reported that endometrial fold extension can lead to uterine lumen closure in pigs.					
34957619	10	64	from	role	1356:1359	arg1	migration					1422:1430	collective epithelial migration	1400:1430	collective epithelial migration	1400:1430	Subsequently, the role of ST6GAL1-mediated 2,6-sialylation in collective epithelial migration was demonstrated.					
34957619	10	65	theme	2,6-sialylation	1381:1395	arg1	role					1356:1359	the role	1352:1359	the role of ST6GAL1-mediated 2,6-sialylation in collective epithelial migration	1352:1430	Subsequently, the role of ST6GAL1-mediated 2,6-sialylation in collective epithelial migration was demonstrated.					
34957619	12	66	theme	uterine	1809:1815	arg1	LE					1817:1818	uterine LE	1809:1818	uterine LE	1809:1818	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	2	67	theme	embryo	217:222	arg1	adhesion					224:231	embryo adhesion	217:231	embryo adhesion	217:231	The uterine lumen closure contributes to embryo adhesion to the uterus, but its underlying mechanisms are largely unknown.					
34957619	12	68	theme	fold	1751:1754	arg1	extension					1756:1764	endometrial fold extension	1739:1764	endometrial fold extension	1739:1764	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	8	69	theme	luminal	996:1002	arg1	LE					1016:1017	LE	1016:1017	LE	1016:1017	RESULTS We observed that uterine luminal epithelium (LE) migrated collectively during endometrial fold extension.					
34957619	8	69	theme	luminal	996:1002	arg1	epithelium					1004:1013	uterine luminal epithelium	988:1013	uterine luminal epithelium (LE)	988:1018	RESULTS We observed that uterine luminal epithelium (LE) migrated collectively during endometrial fold extension.					
34957619	9	70	theme	compositions	1138:1149	arg1	number					1119:1124	a large number	1111:1124	a large number of N-glycan compositions	1111:1149	For the first time, we identified a large number of N-glycan compositions from endometrium during implantation using MALDI-TOF MS. Notably, the α2,6-linked sialic acid and ST6GAL1 were highly expressed in uterine LE when the endometrial folds extended greatly.					
34957619	11	71	theme	LE	1572:1573	arg1	migration					1551:1559	collective migration	1540:1559	collective migration of uterine LE	1540:1573	Finally, we found that ST6GAL1-mediated α2,6-sialylation of E-cadherin may participate in collective migration of uterine LE.					
34957619	11	72	theme	E-cadherin	1510:1519	arg1	α2,6-sialylation					1490:1505	ST6GAL1-mediated α2,6-sialylation	1473:1505	ST6GAL1-mediated α2,6-sialylation of E-cadherin	1473:1519	Finally, we found that ST6GAL1-mediated α2,6-sialylation of E-cadherin may participate in collective migration of uterine LE.					
34957619	3	73	theme	fold	348:351	arg1	extension					353:361	endometrial fold extension	336:361	endometrial fold extension	336:361	Our previous study has reported that endometrial fold extension can lead to uterine lumen closure in pigs.					
34957619	2	74	theme	underlying	256:265	arg1	mechanisms					267:276	its underlying mechanisms	252:276	its underlying mechanisms	252:276	The uterine lumen closure contributes to embryo adhesion to the uterus, but its underlying mechanisms are largely unknown.					
34957619	8	75	theme	uterine	988:994	arg1	LE					1016:1017	LE	1016:1017	LE	1016:1017	RESULTS We observed that uterine luminal epithelium (LE) migrated collectively during endometrial fold extension.					
34957619	8	75	theme	uterine	988:994	arg1	epithelium					1004:1013	uterine luminal epithelium	988:1013	uterine luminal epithelium (LE)	988:1018	RESULTS We observed that uterine luminal epithelium (LE) migrated collectively during endometrial fold extension.					
34957619	1	76	theme	prenatal	156:163	arg1	mortality					165:173	prenatal mortality	156:173	prenatal mortality	156:173	OBJECTIVES Implantation failure is a major cause of prenatal mortality.					
34957619	7	77	theme	molecular	946:954	arg1	basis					956:960	the molecular basis	942:960	the molecular basis	942:960	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34957619	12	78	theme	ST6GAL1-mediated	1663:1678	arg1	α2,6-sialylation					1680:1695	ST6GAL1-mediated α2,6-sialylation	1663:1695	ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules	1663:1722	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	12	79	theme	closure	1635:1641	arg1	mechanism					1608:1616	a mechanism	1606:1616	a mechanism of uterine lumen closure	1606:1641	CONCLUSIONS The study reveals a mechanism of uterine lumen closure by identifying that ST6GAL1-mediated α2,6-sialylation of cell adhesion molecules contributes to endometrial fold extension through regulating collective migration of uterine LE.					
34957619	1	80	theme	mortality	165:173	arg1	cause					147:151	a major cause	139:151	a major cause of prenatal mortality	139:173	OBJECTIVES Implantation failure is a major cause of prenatal mortality.					
34957619	1	80	theme	mortality	165:173	arg1	failure					128:134	OBJECTIVES Implantation failure	104:134	OBJECTIVES Implantation failure	104:134	OBJECTIVES Implantation failure is a major cause of prenatal mortality.					
34957619	7	81	theme	siRNA	776:780	arg1	transfection					782:793	siRNA transfection	776:793	siRNA transfection	776:793	Histochemistry, siRNA transfection, Western blotting, lectin immumoprecipitation, mass spectrometry and assays of wounding healing and cell aggregation were performed to investigate the molecular basis.					
34399182	6	0	contain	had	866:868	arg1	MAWP-60p					857:864	MAWP-60p	857:864	MAWP-60p	857:864	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	0	contain	had	866:868	arg2	viscosity					882:890	the highest viscosity	870:890	the highest viscosity	870:890	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	0	1	from	architecture	14:25	arg1	albuminosa					97:106	albuminosa	97:106	albuminosa	97:106	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
34399182	0	2	theme	solution	31:38	arg1	properties					40:49	solution properties	31:49	solution properties	31:49	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
34399182	3	3	from	abundant	166:173	arg1	polysaccharides					185:199	active polysaccharides	178:199	active polysaccharides	178:199	Pegler is abundant in active polysaccharides, but little is known about their structures and solution properties.					
34399182	4	4	theme	linkage	499:505	arg1	patterns					507:514	linkage patterns	499:514	linkage patterns	499:514	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	6	5	theme	gyration	951:958	arg1	radius					941:946	mean square radius	929:946	mean square radius of gyration (Rg) and O-methyl group attached to the backbone	929:1007	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	5	theme	gyration	951:958	arg1	weight					921:926	its highest molecular weight	899:926	its highest molecular weight	899:926	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	5	6	theme	unreported	679:688	arg1	glucomannogalactan					690:707	a previously unreported glucomannogalactan	666:707	a previously unreported glucomannogalactan	666:707	Methylation and NMR analysis revealed MAWP-60p and MAWP-70 were a 3-O-methylated glucomannogalactan and a previously unreported glucomannogalactan, whereas MAWP-80 was elucidated as a branched galactoglucan.					
34399182	7	7	dep	flexibility	1122:1132	arg1	the					1118:1120	the	1118:1120	the	1118:1120	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	4	8	from	albuminosa	325:334	arg1	polysaccharides					301:315	water-extracted polysaccharides	285:315	water-extracted polysaccharides from M. albuminosa (MAWP)	285:341	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	4	9	theme	structural	383:392	arg1	heterogeneity					394:406	structural heterogeneity	383:406	structural heterogeneity	383:406	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	4	10	theme	monosaccharide	468:481	arg1	composition					483:493	monosaccharide composition	468:493	monosaccharide composition	468:493	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	6	11	theme	coil	812:815	arg1	conformation					817:828	random coil conformation	805:828	random coil conformation in aqueous solution	805:848	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	12	theme	square	934:939	arg1	radius					941:946	mean square radius	929:946	mean square radius of gyration (Rg) and O-methyl group attached to the backbone	929:1007	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	12	theme	square	934:939	arg1	weight					921:926	its highest molecular weight	899:926	its highest molecular weight	899:926	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	5	13	theme	3-O-methylated	628:641	arg1	glucomannogalactan					643:660	a 3-O-methylated glucomannogalactan	626:660	a 3-O-methylated glucomannogalactan	626:660	Methylation and NMR analysis revealed MAWP-60p and MAWP-70 were a 3-O-methylated glucomannogalactan and a previously unreported glucomannogalactan, whereas MAWP-80 was elucidated as a branched galactoglucan.					
34399182	5	13	theme	3-O-methylated	628:641	arg1	MAWP-70					613:619	MAWP-70	613:619	MAWP-70	613:619	Methylation and NMR analysis revealed MAWP-60p and MAWP-70 were a 3-O-methylated glucomannogalactan and a previously unreported glucomannogalactan, whereas MAWP-80 was elucidated as a branched galactoglucan.					
34399182	0	14	from	properties	40:49	arg1	albuminosa					97:106	albuminosa	97:106	albuminosa	97:106	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
34399182	3	15	theme	solution	249:256	arg1	properties					258:267	solution properties	249:267	solution properties	249:267	Pegler is abundant in active polysaccharides, but little is known about their structures and solution properties.					
34399182	4	16	theme	water-extracted	285:299	arg1	polysaccharides					301:315	water-extracted polysaccharides	285:315	water-extracted polysaccharides from M. albuminosa (MAWP)	285:341	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	3	17	theme	active	178:183	arg1	polysaccharides					185:199	active polysaccharides	178:199	active polysaccharides	178:199	Pegler is abundant in active polysaccharides, but little is known about their structures and solution properties.					
34399182	6	18	theme	aqueous	833:839	arg1	solution					841:848	aqueous solution	833:848	aqueous solution	833:848	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	5	19	theme	branched	746:753	arg1	MAWP-80					718:724	MAWP-80	718:724	MAWP-80	718:724	Methylation and NMR analysis revealed MAWP-60p and MAWP-70 were a 3-O-methylated glucomannogalactan and a previously unreported glucomannogalactan, whereas MAWP-80 was elucidated as a branched galactoglucan.					
34399182	5	19	theme	branched	746:753	arg1	galactoglucan					755:767	a branched galactoglucan	744:767	a branched galactoglucan	744:767	Methylation and NMR analysis revealed MAWP-60p and MAWP-70 were a 3-O-methylated glucomannogalactan and a previously unreported glucomannogalactan, whereas MAWP-80 was elucidated as a branched galactoglucan.					
34399182	0	20	from	albuminosa	97:106	arg1	architecture					14:25	architecture	14:25	architecture	14:25	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
34399182	0	20	from	albuminosa	97:106	arg1	properties					40:49	solution properties	31:49	solution properties	31:49	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
34399182	0	20	from	albuminosa	97:106	arg1	fractions					69:77	polysaccharide fractions	54:77	polysaccharide fractions from Macrolepiota albuminosa	54:106	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
34399182	7	21	theme	chains	1197:1202	arg1	stereochemistry					1154:1168	stereochemistry	1154:1168	stereochemistry	1154:1168	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	21	theme	chains	1197:1202	arg1	flexibility					1122:1132	flexibility	1122:1132	flexibility	1122:1132	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	21	theme	chains	1197:1202	arg1	size					1145:1148	molecular size	1135:1148	molecular size	1135:1148	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	6	22	theme	mean	929:932	arg1	radius					941:946	mean square radius	929:946	mean square radius of gyration (Rg) and O-methyl group attached to the backbone	929:1007	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	22	theme	mean	929:932	arg1	weight					921:926	its highest molecular weight	899:926	its highest molecular weight	899:926	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	23	theme	molecular	911:919	arg1	radius					941:946	mean square radius	929:946	mean square radius of gyration (Rg) and O-methyl group attached to the backbone	929:1007	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	23	theme	molecular	911:919	arg1	weight					921:926	its highest molecular weight	899:926	its highest molecular weight	899:926	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	7	24	theme	mushroom	1173:1180	arg1	chains					1197:1202	mushroom polysaccharide chains	1173:1202	mushroom polysaccharide chains	1173:1202	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	4	25	with	fractions	368:376	arg1	heterogeneity					394:406	structural heterogeneity	383:406	structural heterogeneity	383:406	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	6	26	theme	group	978:982	arg1	radius					941:946	mean square radius	929:946	mean square radius of gyration (Rg) and O-methyl group attached to the backbone	929:1007	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	26	theme	group	978:982	arg1	weight					921:926	its highest molecular weight	899:926	its highest molecular weight	899:926	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	4	27	from	diversity	437:445	arg1	composition					483:493	monosaccharide composition	468:493	monosaccharide composition	468:493	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	4	27	from	diversity	437:445	arg1	patterns					507:514	linkage patterns	499:514	linkage patterns	499:514	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	4	27	from	diversity	437:445	arg1	weight					460:465	molecular weight	450:465	molecular weight	450:465	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	6	28	theme	highest	903:909	arg1	radius					941:946	mean square radius	929:946	mean square radius of gyration (Rg) and O-methyl group attached to the backbone	929:1007	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	28	theme	highest	903:909	arg1	weight					921:926	its highest molecular weight	899:926	its highest molecular weight	899:926	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	0	29	theme	polysaccharide	54:67	arg1	fractions					69:77	polysaccharide fractions	54:77	polysaccharide fractions from Macrolepiota albuminosa	54:106	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
34399182	6	30	theme	O-methyl	969:976	arg1	group					978:982	O-methyl group	969:982	O-methyl group attached to the backbone	969:1007	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	4	31	theme	molecular	450:458	arg1	weight					460:465	molecular weight	450:465	molecular weight	450:465	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	5	32	theme	Methylation	562:572	arg1	analysis					582:589	Methylation and NMR analysis	562:589	analysis	582:589	Methylation and NMR analysis revealed MAWP-60p and MAWP-70 were a 3-O-methylated glucomannogalactan and a previously unreported glucomannogalactan, whereas MAWP-80 was elucidated as a branched galactoglucan.					
34399182	7	33	theme	major	1096:1100	arg1	composition					1047:1057	monosaccharide composition	1032:1057	monosaccharide composition	1032:1057	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	33	theme	major	1096:1100	arg1	weight					1024:1029	The molecular weight	1010:1029	The molecular weight	1010:1029	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	33	theme	major	1096:1100	arg1	contributors					1102:1113	the major contributors	1092:1113	the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains	1092:1202	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	33	theme	major	1096:1100	arg1	linkages					1074:1081	glycosidic linkages	1063:1081	glycosidic linkages	1063:1081	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	34	theme	polysaccharide	1182:1195	arg1	chains					1197:1202	mushroom polysaccharide chains	1173:1202	mushroom polysaccharide chains	1173:1202	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	35	theme	molecular	1135:1143	arg1	size					1145:1148	molecular size	1135:1148	molecular size	1135:1148	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	6	36	theme	random	805:810	arg1	conformation					817:828	random coil conformation	805:828	random coil conformation in aqueous solution	805:848	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	3	37	from	polysaccharides	185:199	arg1	abundant					166:173	abundant	166:173	abundant	166:173	Pegler is abundant in active polysaccharides, but little is known about their structures and solution properties.					
34399182	4	38	theme	solution	541:548	arg1	properties					550:559	their solution properties	535:559	their solution properties	535:559	In this study, water-extracted polysaccharides from M. albuminosa (MAWP) were purified into three fractions with structural heterogeneity, which was attributed to the diversity in molecular weight, monosaccharide composition and linkage patterns, further affecting their solution properties.					
34399182	7	39	theme	glycosidic	1063:1072	arg1	composition					1047:1057	monosaccharide composition	1032:1057	monosaccharide composition	1032:1057	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	39	theme	glycosidic	1063:1072	arg1	weight					1024:1029	The molecular weight	1010:1029	The molecular weight	1010:1029	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	39	theme	glycosidic	1063:1072	arg1	contributors					1102:1113	the major contributors	1092:1113	the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains	1092:1202	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	39	theme	glycosidic	1063:1072	arg1	linkages					1074:1081	glycosidic linkages	1063:1081	glycosidic linkages	1063:1081	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	5	40	theme	NMR	578:580	arg1	analysis					582:589	Methylation and NMR analysis	562:589	analysis	582:589	Methylation and NMR analysis revealed MAWP-60p and MAWP-70 were a 3-O-methylated glucomannogalactan and a previously unreported glucomannogalactan, whereas MAWP-80 was elucidated as a branched galactoglucan.					
34399182	6	41	from	conformation	817:828	arg1	solution					841:848	aqueous solution	833:848	aqueous solution	833:848	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	7	42	theme	monosaccharide	1032:1045	arg1	contributors					1102:1113	the major contributors	1092:1113	the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains	1092:1202	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	42	theme	monosaccharide	1032:1045	arg1	weight					1024:1029	The molecular weight	1010:1029	The molecular weight	1010:1029	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	42	theme	monosaccharide	1032:1045	arg1	composition					1047:1057	monosaccharide composition	1032:1057	monosaccharide composition	1032:1057	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	42	theme	monosaccharide	1032:1045	arg1	linkages					1074:1081	glycosidic linkages	1063:1081	glycosidic linkages	1063:1081	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	0	43	dep	architecture	14:25	arg1	the					10:12	the	10:12	the	10:12	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
34399182	7	44	theme	molecular	1014:1022	arg1	composition					1047:1057	monosaccharide composition	1032:1057	monosaccharide composition	1032:1057	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	44	theme	molecular	1014:1022	arg1	weight					1024:1029	The molecular weight	1010:1029	The molecular weight	1010:1029	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	44	theme	molecular	1014:1022	arg1	contributors					1102:1113	the major contributors	1092:1113	the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains	1092:1202	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	7	44	theme	molecular	1014:1022	arg1	linkages					1074:1081	glycosidic linkages	1063:1081	glycosidic linkages	1063:1081	The molecular weight, monosaccharide composition and glycosidic linkages might be the major contributors to the flexibility, molecular size and stereochemistry of mushroom polysaccharide chains.					
34399182	6	45	theme	highest	874:880	arg1	viscosity					882:890	the highest viscosity	870:890	the highest viscosity	870:890	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	46	attach	attached	984:991	arg2	group					978:982	O-methyl group	969:982	O-methyl group attached to the backbone	969:1007	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	6	46	attach	attached	984:991	arg1	backbone					1000:1007	the backbone	996:1007	the backbone	996:1007	Besides, three fractions exhibited random coil conformation in aqueous solution, while MAWP-60p had the highest viscosity due to its highest molecular weight, mean square radius of gyration (Rg) and O-methyl group attached to the backbone.					
34399182	0	47	theme	fractions	69:77	arg1	architecture					14:25	architecture	14:25	architecture	14:25	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
34399182	0	47	theme	fractions	69:77	arg1	properties					40:49	solution properties	31:49	solution properties	31:49	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
34399182	0	48	dep	Revealing	0:8	arg1	Berk					109:112	Berk	109:112	Berk	109:112	Revealing the architecture and solution properties of polysaccharide fractions from Macrolepiota albuminosa (Berk.)					
32708560	9	0	theme	DLC	1220:1222	arg1	content					1224:1230	the DLC content	1216:1230	the DLC content	1216:1230	As the DLC content increased, the water resistance first increased, then decreased.					
32708560	10	1	theme	tensile	1309:1315	arg1	strength					1317:1324	The highest tensile strength	1297:1324	The highest tensile strength	1297:1324	The highest tensile strength and elongation at break reached 5.26 MPa and 111.25%, respectively, and the highest contact angle was about 90.7°.					
32708560	3	2	theme	X-ray	478:482	arg1	diffraction					484:494	X-ray diffraction	478:494	X-ray diffraction (XRD)	478:500	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	3	2	theme	X-ray	478:482	arg1	XRD					497:499	XRD	497:499	XRD	497:499	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	4	3	theme	oxidation	793:801	arg1	damage					803:808	oxidation damage	793:808	oxidation damage to the LC	793:818	XRD showed that the crystallinity of the DLC decreased due to oxidation damage to the LC.					
32708560	2	4	theme	DLC-reinforced	326:339	arg1	composites					345:354	the DLC-reinforced TPS composites	322:354	the DLC-reinforced TPS composites	322:354	Then, the DLC-reinforced TPS composites were prepared by an extrusion and injection process using glycerol as a plasticizer.					
32708560	6	5	theme	composite	991:999	arg1	strength					967:974	The tensile strength	955:974	The tensile strength of the DLC/TPS composite	955:999	The tensile strength of the DLC/TPS composite reached a maximum at a DLC content of 3 wt.					
32708560	3	6	theme	scanning	506:513	arg1	SEM					536:538	SEM	536:538	SEM	536:538	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	3	6	theme	scanning	506:513	arg1	microscopy					524:533	scanning electron microscopy	506:533	scanning electron microscopy (SEM)	506:539	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	6	7	theme	wt	1041:1042	arg1	content					1028:1034	a DLC content	1022:1034	a DLC content of 3 wt	1022:1042	The tensile strength of the DLC/TPS composite reached a maximum at a DLC content of 3 wt.					
32708560	3	8	theme	electron	515:522	arg1	SEM					536:538	SEM	536:538	SEM	536:538	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	3	8	theme	electron	515:522	arg1	microscopy					524:533	scanning electron microscopy	506:533	scanning electron microscopy (SEM)	506:539	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	2	9	theme	TPS	341:343	arg1	composites					345:354	the DLC-reinforced TPS composites	322:354	the DLC-reinforced TPS composites	322:354	Then, the DLC-reinforced TPS composites were prepared by an extrusion and injection process using glycerol as a plasticizer.					
32708560	8	10	theme	neat	1203:1206	arg1	TPS					1208:1210	the neat TPS	1199:1210	the neat TPS	1199:1210	The DLC/TPS composites had better thermal stability than the neat TPS.					
32708560	10	11	theme	contact	1410:1416	arg1	angle					1418:1422	the highest contact angle	1398:1422	the highest contact angle	1398:1422	The highest tensile strength and elongation at break reached 5.26 MPa and 111.25%, respectively, and the highest contact angle was about 90.7°.					
32708560	10	11	theme	contact	1410:1416	arg1	90.7°					1434:1438	90.7°	1434:1438	90.7°	1434:1438	The highest tensile strength and elongation at break reached 5.26 MPa and 111.25%, respectively, and the highest contact angle was about 90.7°.					
32708560	3	12	theme	mechanical	669:678	arg1	properties					680:689	mechanical properties	669:689	mechanical properties	669:689	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	10	13	theme	highest	1301:1307	arg1	strength					1317:1324	The highest tensile strength	1297:1324	The highest tensile strength	1297:1324	The highest tensile strength and elongation at break reached 5.26 MPa and 111.25%, respectively, and the highest contact angle was about 90.7°.					
32708560	2	14	theme	extrusion	376:384	arg1	process					400:406	an extrusion and injection process	373:406	an extrusion and injection process using glycerol as a plasticizer	373:438	Then, the DLC-reinforced TPS composites were prepared by an extrusion and injection process using glycerol as a plasticizer.					
32708560	10	15	theme	highest	1402:1408	arg1	angle					1418:1422	the highest contact angle	1398:1422	the highest contact angle	1398:1422	The highest tensile strength and elongation at break reached 5.26 MPa and 111.25%, respectively, and the highest contact angle was about 90.7°.					
32708560	10	15	theme	highest	1402:1408	arg1	90.7°					1434:1438	90.7°	1434:1438	90.7°	1434:1438	The highest tensile strength and elongation at break reached 5.26 MPa and 111.25%, respectively, and the highest contact angle was about 90.7°.					
32708560	3	16	theme	SEM	657:659	arg1	images					661:666	SEM images	657:666	SEM images	657:666	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	4	17	theme	DLC	772:774	arg1	crystallinity					751:763	the crystallinity	747:763	the crystallinity of the DLC	747:774	XRD showed that the crystallinity of the DLC decreased due to oxidation damage to the LC.					
32708560	5	18	theme	low	890:892	arg1	DLC					905:907	DLC	905:907	DLC	905:907	SEM showed good dispersion of the DLC in the continuous TPS phase at low amounts of DLC, which related to good mechanical properties.					
32708560	5	18	theme	low	890:892	arg1	amounts					894:900	low amounts	890:900	low amounts of DLC, which related to good mechanical properties	890:952	SEM showed good dispersion of the DLC in the continuous TPS phase at low amounts of DLC, which related to good mechanical properties.					
32708560	6	19	theme	DLC/TPS	983:989	arg1	composite					991:999	the DLC/TPS composite	979:999	the DLC/TPS composite	979:999	The tensile strength of the DLC/TPS composite reached a maximum at a DLC content of 3 wt.					
32708560	0	20	theme	Thermoplastic	10:22	arg1	Composites					31:40	Bio-Based Thermoplastic Starch Composites	0:40	Bio-Based Thermoplastic Starch Composites	0:40	Bio-Based Thermoplastic Starch Composites Reinforced by Dialdehyde Lignocellulose.					
32708560	3	21	theme	images	661:666	arg1	evaluation					643:652	the evaluation	639:652	the evaluation of SEM images, mechanical properties, thermal stability, and contact angles	639:728	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	5	22	theme	DLC	905:907	arg1	DLC					905:907	DLC	905:907	DLC	905:907	SEM showed good dispersion of the DLC in the continuous TPS phase at low amounts of DLC, which related to good mechanical properties.					
32708560	5	22	theme	DLC	905:907	arg1	amounts					894:900	low amounts	890:900	low amounts of DLC, which related to good mechanical properties	890:952	SEM showed good dispersion of the DLC in the continuous TPS phase at low amounts of DLC, which related to good mechanical properties.					
32708560	5	23	theme	DLC	855:857	arg1	dispersion					837:846	good dispersion	832:846	good dispersion of the DLC	832:857	SEM showed good dispersion of the DLC in the continuous TPS phase at low amounts of DLC, which related to good mechanical properties.					
32708560	7	24	dep	%	1044:1044	arg1	while					1047:1051	while	1047:1051	while	1047:1051	%, while the elongation at break of the DLC/TPS composites increased with increasing DLC content.					
32708560	0	25	theme	Bio-Based	0:8	arg1	Composites					31:40	Bio-Based Thermoplastic Starch Composites	0:40	Bio-Based Thermoplastic Starch Composites	0:40	Bio-Based Thermoplastic Starch Composites Reinforced by Dialdehyde Lignocellulose.					
32708560	3	26	theme	thermal	692:698	arg1	stability					700:708	thermal stability	692:708	thermal stability	692:708	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	1	27	theme	novel	183:187	arg1	reinforcement					189:201	a novel reinforcement	181:201	a novel reinforcement of dialdehyde lignocellulose (DLC)	181:236	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	1	28	theme	lignocellulose	272:285	arg1	oxidation					259:267	the oxidation	255:267	the oxidation of lignocellulose (LC) using sodium periodate	255:313	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	8	29	theme	better	1169:1174	arg1	stability					1184:1192	better thermal stability	1169:1192	better thermal stability	1169:1192	The DLC/TPS composites had better thermal stability than the neat TPS.					
32708560	1	30	theme	mechanical	107:116	arg1	properties					118:127	mechanical properties	107:127	mechanical properties	107:127	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	0	31	theme	Starch	24:29	arg1	Composites					31:40	Bio-Based Thermoplastic Starch Composites	0:40	Bio-Based Thermoplastic Starch Composites	0:40	Bio-Based Thermoplastic Starch Composites Reinforced by Dialdehyde Lignocellulose.					
32708560	3	32	theme	properties	680:689	arg1	evaluation					643:652	the evaluation	639:652	the evaluation of SEM images, mechanical properties, thermal stability, and contact angles	639:728	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	5	33	theme	continuous	866:875	arg1	phase					881:885	the continuous TPS phase	862:885	the continuous TPS phase	862:885	SEM showed good dispersion of the DLC in the continuous TPS phase at low amounts of DLC, which related to good mechanical properties.					
32708560	1	34	theme	dialdehyde	206:215	arg1	DLC					233:235	DLC	233:235	DLC	233:235	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	1	34	theme	dialdehyde	206:215	arg1	lignocellulose					217:230	dialdehyde lignocellulose	206:230	dialdehyde lignocellulose (DLC)	206:236	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	7	35	from	break	1071:1075	arg1	elongation					1057:1066	the elongation	1053:1066	the elongation at break of the DLC/TPS composites	1053:1101	%, while the elongation at break of the DLC/TPS composites increased with increasing DLC content.					
32708560	3	36	from	effects	550:556	arg1	properties					580:589	the properties	576:589	the properties of the DLC/TPS composites	576:615	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	2	37	theme	injection	390:398	arg1	process					400:406	an extrusion and injection process	373:406	an extrusion and injection process using glycerol as a plasticizer	373:438	Then, the DLC-reinforced TPS composites were prepared by an extrusion and injection process using glycerol as a plasticizer.					
32708560	3	38	theme	contact	715:721	arg1	angles					723:728	contact angles	715:728	contact angles	715:728	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	8	39	theme	DLC/TPS	1146:1152	arg1	composites					1154:1163	The DLC/TPS composites	1142:1163	The DLC/TPS composites	1142:1163	The DLC/TPS composites had better thermal stability than the neat TPS.					
32708560	1	40	theme	lignocellulose	217:230	arg1	reinforcement					189:201	a novel reinforcement	181:201	a novel reinforcement of dialdehyde lignocellulose (DLC)	181:236	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	3	41	theme	angles	723:728	arg1	evaluation					643:652	the evaluation	639:652	the evaluation of SEM images, mechanical properties, thermal stability, and contact angles	639:728	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	3	42	theme	stability	700:708	arg1	evaluation					643:652	the evaluation	639:652	the evaluation of SEM images, mechanical properties, thermal stability, and contact angles	639:728	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	5	43	theme	good	832:835	arg1	dispersion					837:846	good dispersion	832:846	good dispersion of the DLC	832:857	SEM showed good dispersion of the DLC in the continuous TPS phase at low amounts of DLC, which related to good mechanical properties.					
32708560	0	44	theme	Dialdehyde	56:65	arg1	Lignocellulose					67:80	Dialdehyde Lignocellulose	56:80	Dialdehyde Lignocellulose	56:80	Bio-Based Thermoplastic Starch Composites Reinforced by Dialdehyde Lignocellulose.					
32708560	1	45	theme	sodium	298:303	arg1	periodate					305:313	sodium periodate	298:313	sodium periodate	298:313	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	6	46	theme	tensile	959:965	arg1	strength					967:974	The tensile strength	955:974	The tensile strength of the DLC/TPS composite	955:999	The tensile strength of the DLC/TPS composite reached a maximum at a DLC content of 3 wt.					
32708560	5	47	theme	mechanical	932:941	arg1	properties					943:952	good mechanical properties	927:952	good mechanical properties	927:952	SEM showed good dispersion of the DLC in the continuous TPS phase at low amounts of DLC, which related to good mechanical properties.					
32708560	5	48	theme	good	927:930	arg1	properties					943:952	good mechanical properties	927:952	good mechanical properties	927:952	SEM showed good dispersion of the DLC in the continuous TPS phase at low amounts of DLC, which related to good mechanical properties.					
32708560	9	49	dep	increased	1270:1278	arg1	decreased					1286:1294	decreased	1286:1294	decreased	1286:1294	As the DLC content increased, the water resistance first increased, then decreased.					
32708560	1	50	theme	water	133:137	arg1	resistance					139:148	water resistance	133:148	water resistance	133:148	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	7	51	theme	DLC	1129:1131	arg1	content					1133:1139	DLC content	1129:1139	DLC content	1129:1139	%, while the elongation at break of the DLC/TPS composites increased with increasing DLC content.					
32708560	3	52	theme	content	565:571	arg1	effects					550:556	the effects	546:556	the effects of DLC content on the properties of the DLC/TPS composites	546:615	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	8	53	theme	thermal	1176:1182	arg1	stability					1184:1192	better thermal stability	1169:1192	better thermal stability	1169:1192	The DLC/TPS composites had better thermal stability than the neat TPS.					
32708560	8	54	contain	had	1165:1167	arg1	composites					1154:1163	The DLC/TPS composites	1142:1163	The DLC/TPS composites	1142:1163	The DLC/TPS composites had better thermal stability than the neat TPS.					
32708560	8	54	contain	had	1165:1167	arg2	stability					1184:1192	better thermal stability	1169:1192	better thermal stability	1169:1192	The DLC/TPS composites had better thermal stability than the neat TPS.					
32708560	3	55	theme	DLC/TPS	598:604	arg1	composites					606:615	the DLC/TPS composites	594:615	the DLC/TPS composites	594:615	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	5	56	theme	TPS	877:879	arg1	phase					881:885	the continuous TPS phase	862:885	the continuous TPS phase	862:885	SEM showed good dispersion of the DLC in the continuous TPS phase at low amounts of DLC, which related to good mechanical properties.					
32708560	1	57	theme	starch	167:172	arg1	resistance					139:148	water resistance	133:148	water resistance	133:148	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	1	57	theme	starch	167:172	arg1	properties					118:127	mechanical properties	107:127	mechanical properties	107:127	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	6	58	theme	DLC	1024:1026	arg1	content					1028:1034	a DLC content	1022:1034	a DLC content of 3 wt	1022:1042	The tensile strength of the DLC/TPS composite reached a maximum at a DLC content of 3 wt.					
32708560	9	59	theme	water	1247:1251	arg1	resistance					1253:1262	the water resistance	1243:1262	the water resistance	1243:1262	As the DLC content increased, the water resistance first increased, then decreased.					
32708560	7	60	theme	DLC/TPS	1084:1090	arg1	composites					1092:1101	the DLC/TPS composites	1080:1101	the DLC/TPS composites	1080:1101	%, while the elongation at break of the DLC/TPS composites increased with increasing DLC content.					
32708560	3	61	theme	composites	606:615	arg1	properties					580:589	the properties	576:589	the properties of the DLC/TPS composites	576:615	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	3	62	dep	DLC	445:447	arg1	The					441:443	The	441:443	The	441:443	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	1	63	theme	thermoplastic	153:165	arg1	starch					167:172	thermoplastic starch	153:172	thermoplastic starch (TPS)	153:178	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	1	63	theme	thermoplastic	153:165	arg1	TPS					175:177	TPS	175:177	TPS	175:177	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
32708560	10	64	from	break	1344:1348	arg1	elongation					1330:1339	elongation	1330:1339	elongation	1330:1339	The highest tensile strength and elongation at break reached 5.26 MPa and 111.25%, respectively, and the highest contact angle was about 90.7°.					
32708560	10	64	from	break	1344:1348	arg1	strength					1317:1324	The highest tensile strength	1297:1324	The highest tensile strength	1297:1324	The highest tensile strength and elongation at break reached 5.26 MPa and 111.25%, respectively, and the highest contact angle was about 90.7°.					
32708560	7	65	theme	composites	1092:1101	arg1	elongation					1057:1066	the elongation	1053:1066	the elongation at break of the DLC/TPS composites	1053:1101	%, while the elongation at break of the DLC/TPS composites increased with increasing DLC content.					
32708560	3	66	theme	DLC	561:563	arg1	content					565:571	DLC content	561:571	DLC content	561:571	The DLC and LC were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and the effects of DLC content on the properties of the DLC/TPS composites were investigated via the evaluation of SEM images, mechanical properties, thermal stability, and contact angles.					
32708560	1	67	dep	properties	118:127	arg1	the					103:105	the	103:105	the	103:105	In order to improve the mechanical properties and water resistance of thermoplastic starch (TPS), a novel reinforcement of dialdehyde lignocellulose (DLC) was prepared via the oxidation of lignocellulose (LC) using sodium periodate.					
34560988	4	0	from	application	635:645	arg1	system					694:699	drug delivery system	680:699	drug delivery system	680:699	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	2	1	used	used	400:403	arg2	methods					387:393	two different methods	373:393	two different methods	373:393	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	5	2	theme	in-vitro	879:886	arg1	study					888:892	the in-vitro study	875:892	the in-vitro study	875:892	The result of the in-vitro study was in favor of the release behavior of BC/CMDEAEC composite.					
34560988	6	3	theme	loading	963:969	arg1	data					971:974	The MB loading data	956:974	The MB loading data	956:974	The MB loading data were fitted using Langmuir and Freundlich equations and kinetic behavior of the release was described by Higuchi and Korsmeyer-Peppas models.					
34560988	2	4	theme	fermentation	515:526	arg1	process					528:534	the fermentation process	511:534	the fermentation process	511:534	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	6	5	dep	Higuchi	1081:1087	arg1	models					1110:1115	models	1110:1115	models	1110:1115	The MB loading data were fitted using Langmuir and Freundlich equations and kinetic behavior of the release was described by Higuchi and Korsmeyer-Peppas models.					
34560988	2	6	theme	carboxymethylated	284:300	arg1	cellulose					320:328	carboxymethylated diethylaminoethyl cellulose	284:328	carboxymethylated diethylaminoethyl cellulose (CMDEAEC)	284:338	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	2	6	theme	carboxymethylated	284:300	arg1	CMDEAEC					331:337	CMDEAEC	331:337	CMDEAEC	331:337	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	2	7	theme	diethylaminoethyl	302:318	arg1	cellulose					320:328	carboxymethylated diethylaminoethyl cellulose	284:328	carboxymethylated diethylaminoethyl cellulose (CMDEAEC)	284:338	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	2	7	theme	diethylaminoethyl	302:318	arg1	CMDEAEC					331:337	CMDEAEC	331:337	CMDEAEC	331:337	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	6	8	theme	MB	960:961	arg1	data					971:974	The MB loading data	956:974	The MB loading data	956:974	The MB loading data were fitted using Langmuir and Freundlich equations and kinetic behavior of the release was described by Higuchi and Korsmeyer-Peppas models.					
34560988	5	9	theme	BC/CMDEAEC	934:943	arg1	composite					945:953	BC/CMDEAEC composite	934:953	BC/CMDEAEC composite	934:953	The result of the in-vitro study was in favor of the release behavior of BC/CMDEAEC composite.					
34560988	5	10	theme	study	888:892	arg1	result					865:870	The result	861:870	The result of the in-vitro study	861:892	The result of the in-vitro study was in favor of the release behavior of BC/CMDEAEC composite.					
34560988	4	11	theme	loading	766:772	arg1	capacity					774:781	loading capacity	766:781	loading capacity	766:781	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	1	12	theme	G.	170:171	arg1	xylinus					173:179	G. xylinus	170:179	G. xylinus	170:179	Bacterial cellulose (BC) was produced via the static fermentation process using G. xylinus.					
34560988	2	13	theme	materials	495:503	arg1	addition					423:430	direct addition	416:430	direct addition of the materials to the fermentation medium	416:474	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	2	13	theme	materials	495:503	arg1	addition					479:486	addition	479:486	addition of the materials after the fermentation process	479:534	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	1	14	theme	Bacterial	90:98	arg1	BC					111:112	BC	111:112	BC	111:112	Bacterial cellulose (BC) was produced via the static fermentation process using G. xylinus.					
34560988	1	14	theme	Bacterial	90:98	arg1	cellulose					100:108	Bacterial cellulose	90:108	Bacterial cellulose (BC)	90:113	Bacterial cellulose (BC) was produced via the static fermentation process using G. xylinus.					
34560988	6	15	theme	release	1056:1062	arg1	behavior					1040:1047	kinetic behavior	1032:1047	kinetic behavior of the release	1032:1062	The MB loading data were fitted using Langmuir and Freundlich equations and kinetic behavior of the release was described by Higuchi and Korsmeyer-Peppas models.					
34560988	4	16	theme	BC/CMDEAEC	666:675	arg1	application					635:645	Potential application	625:645	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system	625:699	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	2	17	theme	materials	439:447	arg1	addition					423:430	direct addition	416:430	direct addition of the materials to the fermentation medium	416:474	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	2	17	theme	materials	439:447	arg1	addition					479:486	addition	479:486	addition of the materials after the fermentation process	479:534	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	4	18	theme	delivery	685:692	arg1	system					694:699	drug delivery system	680:699	drug delivery system	680:699	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	2	19	theme	different	377:385	arg1	methods					387:393	two different methods	373:393	two different methods	373:393	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	4	20	theme	model	745:749	arg1	methylene					720:728	methylene blue	720:733	methylene blue (MB)	720:738	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	4	20	theme	model	745:749	arg1	drug					751:754	a model drug	743:754	a model drug where the loading capacity and swelling ratio for the samples were as follows	743:832	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	2	21	theme	direct	416:421	arg1	addition					423:430	direct addition	416:430	direct addition of the materials to the fermentation medium	416:474	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	5	22	theme	composite	945:953	arg1	behavior					922:929	the release behavior	910:929	the release behavior of BC/CMDEAEC composite	910:953	The result of the in-vitro study was in favor of the release behavior of BC/CMDEAEC composite.					
34560988	3	23	theme	composites	567:576	arg1	characteristics					548:562	Structural characteristics	537:562	Structural characteristics of composites	537:576	Structural characteristics of composites were determined using instrumental techniques.					
34560988	0	24	theme	cellulose-based	44:58	arg1	composites					60:69	bacterial cellulose-based composites	34:69	bacterial cellulose-based composites	34:69	Synthesis and characterization of bacterial cellulose-based composites for drug delivery.					
34560988	4	25	theme	drug	680:683	arg1	system					694:699	drug delivery system	680:699	drug delivery system	680:699	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	6	26	theme	Freundlich	1007:1016	arg1	equations					1018:1026	Langmuir and Freundlich equations	994:1026	Langmuir and Freundlich equations	994:1026	The MB loading data were fitted using Langmuir and Freundlich equations and kinetic behavior of the release was described by Higuchi and Korsmeyer-Peppas models.					
34560988	0	27	theme	bacterial	34:42	arg1	composites					60:69	bacterial cellulose-based composites	34:69	bacterial cellulose-based composites	34:69	Synthesis and characterization of bacterial cellulose-based composites for drug delivery.					
34560988	2	28	theme	fermentation	456:467	arg1	medium					469:474	the fermentation medium	452:474	the fermentation medium	452:474	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	4	29	theme	blue	730:733	arg1	drug					751:754	a model drug	743:754	a model drug where the loading capacity and swelling ratio for the samples were as follows	743:832	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	4	29	theme	blue	730:733	arg1	methylene					720:728	methylene blue	720:733	methylene blue (MB)	720:738	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	4	29	theme	blue	730:733	arg1	MB					736:737	MB	736:737	MB	736:737	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	0	30	theme	composites	60:69	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of bacterial cellulose-based composites for drug delivery.					
34560988	0	30	theme	composites	60:69	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of bacterial cellulose-based composites for drug delivery.					
34560988	6	31	theme	kinetic	1032:1038	arg1	behavior					1040:1047	kinetic behavior	1032:1047	kinetic behavior of the release	1032:1062	The MB loading data were fitted using Langmuir and Freundlich equations and kinetic behavior of the release was described by Higuchi and Korsmeyer-Peppas models.					
34560988	3	32	theme	instrumental	600:611	arg1	techniques					613:622	instrumental techniques	600:622	instrumental techniques	600:622	Structural characteristics of composites were determined using instrumental techniques.					
34560988	5	33	theme	release	914:920	arg1	behavior					922:929	the release behavior	910:929	the release behavior of BC/CMDEAEC composite	910:953	The result of the in-vitro study was in favor of the release behavior of BC/CMDEAEC composite.					
34560988	6	34	theme	Langmuir	994:1001	arg1	equations					1018:1026	Langmuir and Freundlich equations	994:1026	Langmuir and Freundlich equations	994:1026	The MB loading data were fitted using Langmuir and Freundlich equations and kinetic behavior of the release was described by Higuchi and Korsmeyer-Peppas models.					
34560988	2	35	theme	diethylaminoethyl	196:212	arg1	cellulose					214:222	diethylaminoethyl cellulose	196:222	diethylaminoethyl cellulose	196:222	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	3	36	theme	Structural	537:546	arg1	characteristics					548:562	Structural characteristics	537:562	Structural characteristics of composites	537:576	Structural characteristics of composites were determined using instrumental techniques.					
34560988	4	37	theme	Potential	625:633	arg1	application					635:645	Potential application	625:645	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system	625:699	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	2	38	theme	carboxymethyl	250:262	arg1	CMC					275:277	CMC	275:277	CMC	275:277	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	2	38	theme	carboxymethyl	250:262	arg1	cellulose					264:272	carboxymethyl cellulose	250:272	carboxymethyl cellulose (CMC)	250:278	Cellulose and diethylaminoethyl cellulose (DEAEC) were converted to carboxymethyl cellulose (CMC) and carboxymethylated diethylaminoethyl cellulose (CMDEAEC) while to prepare the composites, two different methods were used: by either direct addition of the materials to the fermentation medium or addition of the materials after the fermentation process.					
34560988	4	39	theme	BC/CMC	654:659	arg1	application					635:645	Potential application	625:645	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system	625:699	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	1	40	theme	static	136:141	arg1	process					156:162	the static fermentation process	132:162	the static fermentation process using G. xylinus	132:179	Bacterial cellulose (BC) was produced via the static fermentation process using G. xylinus.					
34560988	4	41	theme	BC	650:651	arg1	application					635:645	Potential application	625:645	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system	625:699	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	0	42	theme	drug	75:78	arg1	delivery					80:87	drug delivery	75:87	drug delivery	75:87	Synthesis and characterization of bacterial cellulose-based composites for drug delivery.					
34560988	4	43	theme	swelling	787:794	arg1	ratio					796:800	swelling ratio	787:800	swelling ratio	787:800	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
34560988	1	44	theme	fermentation	143:154	arg1	process					156:162	the static fermentation process	132:162	the static fermentation process using G. xylinus	132:179	Bacterial cellulose (BC) was produced via the static fermentation process using G. xylinus.					
34560988	4	45	dep	capacity	774:781	arg1	the					762:764	the	762:764	the	762:764	Potential application of BC, BC/CMC, and BC/CMDEAEC in drug delivery system was examined using methylene blue (MB) as a model drug where the loading capacity and swelling ratio for the samples were as follows: BC/CMC > BC/CMDEAEC > BC.					
32599241	0	0	theme	fucoidan	81:88	arg1	purification					44:55	the purification	40:55	the purification of low molecular weight fucoidan	40:88	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.					
32599241	6	1	theme	MW	894:895	arg1	distribution					897:908	an estimated MW distribution	881:908	an estimated MW distribution of 8-25 kDa	881:920	SSQC4 was comprising of fucoidan, with an estimated MW distribution of 8-25 kDa.					
32599241	2	2	theme	weight	321:326	arg1	fucoidan					333:340	Low molecular weight (MW) fucoidan	307:340	Low molecular weight (MW) fucoidan	307:340	Low molecular weight (MW) fucoidan is renowned for possessing enhanced antioxidant activities.					
32599241	10	3	dep	safety	1422:1427	arg1	The					1418:1420	The	1418:1420	The	1418:1420	The safety and stability of SSQC4 could be further evaluated to promote its use as a bioactive natural ingredient in UV-protective cosmetics.					
32599241	1	4	theme	suntans	234:240	arg1	layers					211:216	outermost layers	201:216	outermost layers of skin causing suntans, sunburns	201:250	Ultraviolet B (UVB) can induce oxidative damage to outermost layers of skin causing suntans, sunburns, and, in severe cases, blisters leading to photoaging.					
32599241	0	5	theme	weight	74:79	arg1	fucoidan					81:88	low molecular weight fucoidan	60:88	low molecular weight fucoidan	60:88	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.					
32599241	6	6	theme	estimated	884:892	arg1	distribution					897:908	an estimated MW distribution	881:908	an estimated MW distribution of 8-25 kDa	881:920	SSQC4 was comprising of fucoidan, with an estimated MW distribution of 8-25 kDa.					
32599241	2	7	theme	molecular	311:319	arg1	weight					321:326	Low molecular weight	307:326	Low molecular weight (MW) fucoidan	307:340	Low molecular weight (MW) fucoidan is renowned for possessing enhanced antioxidant activities.					
32599241	2	7	theme	molecular	311:319	arg1	MW					329:330	MW	329:330	MW	329:330	Low molecular weight (MW) fucoidan is renowned for possessing enhanced antioxidant activities.					
32599241	9	8	dep	increasing	1315:1324	arg1	identified					1367:1376	identified	1367:1376	was identified as the cause of observed bioactivities	1363:1415	SSQC4 treatment enhanced cellular antioxidant defense by increasing Nrf2 mediated HO-1 generation, which was identified as the cause of observed bioactivities.					
32599241	3	9	from	keratinocytes	612:624	arg1	evaluation					554:563	evaluation	554:563	evaluation of their UVB-protective effects in human HaCaT keratinocytes	554:624	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	9	from	keratinocytes	612:624	arg1	use					434:436	the use	430:436	the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum	430:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	7	10	theme	apoptosis	1100:1108	arg1	pathway					1110:1116	the mitochondria-mediated apoptosis pathway	1074:1116	the mitochondria-mediated apoptosis pathway	1074:1116	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	7	11	theme	intracellular	949:961	arg1	formation					1037:1045	apoptotic body formation	1022:1045	apoptotic body formation	1022:1045	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	7	11	theme	intracellular	949:961	arg1	ROS					963:965	intracellular ROS	949:965	intracellular ROS	949:965	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	7	11	theme	intracellular	949:961	arg1	damage					972:977	DNA damage	968:977	DNA damage	968:977	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	7	11	theme	intracellular	949:961	arg1	loss					980:983	loss	980:983	loss of mitochondrial membrane potential	980:1019	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	9	12	theme	bioactivities	1403:1415	arg1	cause					1385:1389	the cause	1381:1389	the cause of observed bioactivities	1381:1415	SSQC4 treatment enhanced cellular antioxidant defense by increasing Nrf2 mediated HO-1 generation, which was identified as the cause of observed bioactivities.					
32599241	9	12	theme	bioactivities	1403:1415	arg1	which					1357:1361	which	1357:1361	which	1357:1361	SSQC4 treatment enhanced cellular antioxidant defense by increasing Nrf2 mediated HO-1 generation, which was identified as the cause of observed bioactivities.					
32599241	3	13	theme	precipitation	463:475	arg1	evaluation					554:563	evaluation	554:563	evaluation of their UVB-protective effects in human HaCaT keratinocytes	554:624	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	13	theme	precipitation	463:475	arg1	use					434:436	the use	430:436	the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum	430:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	14	from	effects	589:595	arg1	keratinocytes					612:624	human HaCaT keratinocytes	600:624	human HaCaT keratinocytes	600:624	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	15	theme	step	441:444	arg1	precipitation					463:475	step gradient ethanol precipitation	441:475	step gradient ethanol precipitation	441:475	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	5	16	theme	monosaccharide	706:719	arg1	composition					721:731	monosaccharide composition	706:731	monosaccharide composition	706:731	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	5	16	theme	monosaccharide	706:719	arg1	NMR					695:697	1H NMR	692:697	1H NMR	692:697	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	3	17	theme	gradient	446:453	arg1	precipitation					463:475	step gradient ethanol precipitation	441:475	step gradient ethanol precipitation	441:475	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	10	18	theme	bioactive	1503:1511	arg1	ingredient					1521:1530	a bioactive natural ingredient	1501:1530	a bioactive natural ingredient	1501:1530	The safety and stability of SSQC4 could be further evaluated to promote its use as a bioactive natural ingredient in UV-protective cosmetics.					
32599241	5	19	theme	gel	781:783	arg1	electrophoresis					785:799	agarose gel electrophoresis	773:799	agarose gel electrophoresis	773:799	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	7	20	theme	mitochondria-mediated	1078:1098	arg1	pathway					1110:1116	the mitochondria-mediated apoptosis pathway	1074:1116	the mitochondria-mediated apoptosis pathway	1074:1116	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	7	21	theme	cell	1055:1058	arg1	death					1060:1064	cell death	1055:1064	cell death	1055:1064	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	3	22	theme	present	406:412	arg1	study					414:418	The present study	402:418	The present study	402:418	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	0	23	theme	gradient	5:12	arg1	precipitation					22:34	Step gradient alcohol precipitation	0:34	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.	0:148	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.					
32599241	10	24	from	use	1494:1496	arg1	cosmetics					1549:1557	UV-protective cosmetics	1535:1557	UV-protective cosmetics	1535:1557	The safety and stability of SSQC4 could be further evaluated to promote its use as a bioactive natural ingredient in UV-protective cosmetics.					
32599241	0	25	theme	Step	0:3	arg1	precipitation					22:34	Step gradient alcohol precipitation	0:34	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.	0:148	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.					
32599241	10	26	theme	SSQC4	1446:1450	arg1	stability					1433:1441	stability	1433:1441	stability	1433:1441	The safety and stability of SSQC4 could be further evaluated to promote its use as a bioactive natural ingredient in UV-protective cosmetics.					
32599241	10	26	theme	SSQC4	1446:1450	arg1	safety					1422:1427	safety	1422:1427	safety	1422:1427	The safety and stability of SSQC4 could be further evaluated to promote its use as a bioactive natural ingredient in UV-protective cosmetics.					
32599241	3	27	from	evaluation	554:563	arg1	SSQC1-SSQC4					509:519	SSQC1-SSQC4	509:519	SSQC1-SSQC4	509:519	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	27	from	evaluation	554:563	arg1	fractions					498:506	refining fucoidan fractions	480:506	refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum	480:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	27	from	evaluation	554:563	arg1	siliquastrum					537:548	Sargassum siliquastrum	527:548	Sargassum siliquastrum	527:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	27	from	evaluation	554:563	arg1	keratinocytes					612:624	human HaCaT keratinocytes	600:624	human HaCaT keratinocytes	600:624	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	10	28	theme	natural	1513:1519	arg1	ingredient					1521:1530	a bioactive natural ingredient	1501:1530	a bioactive natural ingredient	1501:1530	The safety and stability of SSQC4 could be further evaluated to promote its use as a bioactive natural ingredient in UV-protective cosmetics.					
32599241	10	29	theme	UV-protective	1535:1547	arg1	cosmetics					1549:1557	UV-protective cosmetics	1535:1557	UV-protective cosmetics	1535:1557	The safety and stability of SSQC4 could be further evaluated to promote its use as a bioactive natural ingredient in UV-protective cosmetics.					
32599241	7	30	theme	UVB	935:937	arg1	Exposure					923:930	Exposure	923:930	Exposure of UVB	923:937	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	8	31	theme	exposed	1235:1241	arg1	keratinocytes					1243:1255	UVB exposed keratinocytes	1231:1255	UVB exposed keratinocytes	1231:1255	SSQC4 treatment could dose-dependently attenuate the ROS levels and suppress mitochondria-mediated apoptosis in UVB exposed keratinocytes.					
32599241	2	32	theme	antioxidant	378:388	arg1	activities					390:399	enhanced antioxidant activities	369:399	enhanced antioxidant activities	369:399	Low molecular weight (MW) fucoidan is renowned for possessing enhanced antioxidant activities.					
32599241	0	33	theme	alcohol	14:20	arg1	precipitation					22:34	Step gradient alcohol precipitation	0:34	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.	0:148	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.					
32599241	5	34	theme	1H	692:693	arg1	FTIR					700:703	FTIR	700:703	FTIR	700:703	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	5	34	theme	1H	692:693	arg1	composition					721:731	monosaccharide composition	706:731	monosaccharide composition	706:731	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	5	34	theme	1H	692:693	arg1	NMR					695:697	1H NMR	692:697	1H NMR	692:697	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	9	35	theme	SSQC4	1258:1262	arg1	treatment					1264:1272	SSQC4 treatment	1258:1272	SSQC4 treatment	1258:1272	SSQC4 treatment enhanced cellular antioxidant defense by increasing Nrf2 mediated HO-1 generation, which was identified as the cause of observed bioactivities.					
32599241	4	36	theme	bioactive	673:681	arg1	effects					683:689	the best bioactive effects	664:689	the best bioactive effects	664:689	Among the fractions, SSQC4 indicated the best bioactive effects.					
32599241	8	37	from	apoptosis	1218:1226	arg1	keratinocytes					1243:1255	UVB exposed keratinocytes	1231:1255	UVB exposed keratinocytes	1231:1255	SSQC4 treatment could dose-dependently attenuate the ROS levels and suppress mitochondria-mediated apoptosis in UVB exposed keratinocytes.					
32599241	2	38	theme	enhanced	369:376	arg1	activities					390:399	enhanced antioxidant activities	369:399	enhanced antioxidant activities	369:399	Low molecular weight (MW) fucoidan is renowned for possessing enhanced antioxidant activities.					
32599241	5	39	theme	MW	756:757	arg1	analysis					746:753	HPAEC-PAD analysis	736:753	HPAEC-PAD analysis	736:753	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	5	39	theme	MW	756:757	arg1	estimation					759:768	MW estimation	756:768	MW estimation	756:768	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	1	40	theme	severe	261:266	arg1	cases					268:272	severe cases	261:272	severe cases	261:272	Ultraviolet B (UVB) can induce oxidative damage to outermost layers of skin causing suntans, sunburns, and, in severe cases, blisters leading to photoaging.					
32599241	7	41	theme	body	1032:1035	arg1	formation					1037:1045	apoptotic body formation	1022:1045	apoptotic body formation	1022:1045	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	7	41	theme	body	1032:1035	arg1	ROS					963:965	intracellular ROS	949:965	intracellular ROS	949:965	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	4	42	theme	best	668:671	arg1	effects					683:689	the best bioactive effects	664:689	the best bioactive effects	664:689	Among the fractions, SSQC4 indicated the best bioactive effects.					
32599241	9	43	theme	observed	1394:1401	arg1	bioactivities					1403:1415	observed bioactivities	1394:1415	observed bioactivities	1394:1415	SSQC4 treatment enhanced cellular antioxidant defense by increasing Nrf2 mediated HO-1 generation, which was identified as the cause of observed bioactivities.					
32599241	0	44	theme	protective	130:139	arg1	effects					141:147	its UVB protective effects	122:147	its UVB protective effects	122:147	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.					
32599241	8	45	theme	SSQC4	1119:1123	arg1	treatment					1125:1133	SSQC4 treatment	1119:1133	SSQC4 treatment	1119:1133	SSQC4 treatment could dose-dependently attenuate the ROS levels and suppress mitochondria-mediated apoptosis in UVB exposed keratinocytes.					
32599241	3	46	theme	UVB-protective	574:587	arg1	effects					589:595	their UVB-protective effects	568:595	their UVB-protective effects in human HaCaT keratinocytes	568:624	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	1	47	theme	outermost	201:209	arg1	layers					211:216	outermost layers	201:216	outermost layers of skin causing suntans, sunburns	201:250	Ultraviolet B (UVB) can induce oxidative damage to outermost layers of skin causing suntans, sunburns, and, in severe cases, blisters leading to photoaging.					
32599241	8	48	theme	ROS	1172:1174	arg1	levels					1176:1181	the ROS levels	1168:1181	the ROS levels	1168:1181	SSQC4 treatment could dose-dependently attenuate the ROS levels and suppress mitochondria-mediated apoptosis in UVB exposed keratinocytes.					
32599241	5	49	used	used	806:809	arg2	FTIR					700:703	FTIR	700:703	FTIR	700:703	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	5	49	used	used	806:809	arg2	NMR					695:697	1H NMR	692:697	1H NMR	692:697	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	5	49	used	used	806:809	arg2	composition					721:731	monosaccharide composition	706:731	monosaccharide composition	706:731	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	5	50	theme	HPAEC-PAD	736:744	arg1	analysis					746:753	HPAEC-PAD analysis	736:753	HPAEC-PAD analysis	736:753	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	5	50	theme	HPAEC-PAD	736:744	arg1	estimation					759:768	MW estimation	756:768	MW estimation	756:768	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	7	51	theme	mitochondrial	988:1000	arg1	potential					1011:1019	mitochondrial membrane potential	988:1019	mitochondrial membrane potential	988:1019	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	2	52	theme	Low	307:309	arg1	weight					321:326	Low molecular weight	307:326	Low molecular weight (MW) fucoidan	307:340	Low molecular weight (MW) fucoidan is renowned for possessing enhanced antioxidant activities.					
32599241	2	52	theme	Low	307:309	arg1	MW					329:330	MW	329:330	MW	329:330	Low molecular weight (MW) fucoidan is renowned for possessing enhanced antioxidant activities.					
32599241	5	53	theme	agarose	773:779	arg1	electrophoresis					785:799	agarose gel electrophoresis	773:799	agarose gel electrophoresis	773:799	1H NMR, FTIR, monosaccharide composition by HPAEC-PAD analysis, MW estimation by agarose gel electrophoresis were used to characterize the fractions.					
32599241	6	54	theme	8-25 kDa	913:920	arg1	distribution					897:908	an estimated MW distribution	881:908	an estimated MW distribution of 8-25 kDa	881:920	SSQC4 was comprising of fucoidan, with an estimated MW distribution of 8-25 kDa.					
32599241	7	55	theme	apoptotic	1022:1030	arg1	formation					1037:1045	apoptotic body formation	1022:1045	apoptotic body formation	1022:1045	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	7	55	theme	apoptotic	1022:1030	arg1	ROS					963:965	intracellular ROS	949:965	intracellular ROS	949:965	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	9	56	theme	antioxidant	1292:1302	arg1	defense					1304:1310	cellular antioxidant defense	1283:1310	cellular antioxidant defense	1283:1310	SSQC4 treatment enhanced cellular antioxidant defense by increasing Nrf2 mediated HO-1 generation, which was identified as the cause of observed bioactivities.					
32599241	9	57	theme	cellular	1283:1290	arg1	defense					1304:1310	cellular antioxidant defense	1283:1310	cellular antioxidant defense	1283:1310	SSQC4 treatment enhanced cellular antioxidant defense by increasing Nrf2 mediated HO-1 generation, which was identified as the cause of observed bioactivities.					
32599241	9	58	theme	mediated	1331:1338	arg1	generation					1345:1354	Nrf2 mediated HO-1 generation	1326:1354	Nrf2 mediated HO-1 generation	1326:1354	SSQC4 treatment enhanced cellular antioxidant defense by increasing Nrf2 mediated HO-1 generation, which was identified as the cause of observed bioactivities.					
32599241	3	59	theme	ethanol	455:461	arg1	precipitation					463:475	step gradient ethanol precipitation	441:475	step gradient ethanol precipitation	441:475	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	8	60	theme	mitochondria-mediated	1196:1216	arg1	apoptosis					1218:1226	mitochondria-mediated apoptosis	1196:1226	mitochondria-mediated apoptosis in UVB exposed keratinocytes	1196:1255	SSQC4 treatment could dose-dependently attenuate the ROS levels and suppress mitochondria-mediated apoptosis in UVB exposed keratinocytes.					
32599241	7	61	theme	membrane	1002:1009	arg1	potential					1011:1019	mitochondrial membrane potential	988:1019	mitochondrial membrane potential	988:1019	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	0	62	from	siliquastrum	105:116	arg1	precipitation					22:34	Step gradient alcohol precipitation	0:34	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.	0:148	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.					
32599241	3	63	theme	human	600:604	arg1	keratinocytes					612:624	human HaCaT keratinocytes	600:624	human HaCaT keratinocytes	600:624	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	64	from	use	434:436	arg1	SSQC1-SSQC4					509:519	SSQC1-SSQC4	509:519	SSQC1-SSQC4	509:519	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	64	from	use	434:436	arg1	fractions					498:506	refining fucoidan fractions	480:506	refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum	480:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	64	from	use	434:436	arg1	siliquastrum					537:548	Sargassum siliquastrum	527:548	Sargassum siliquastrum	527:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	64	from	use	434:436	arg1	keratinocytes					612:624	human HaCaT keratinocytes	600:624	human HaCaT keratinocytes	600:624	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	0	65	from	effects	141:147	arg1	precipitation					22:34	Step gradient alcohol precipitation	0:34	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.	0:148	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.					
32599241	7	66	theme	potential	1011:1019	arg1	loss					980:983	loss	980:983	loss of mitochondrial membrane potential	980:1019	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	7	66	theme	potential	1011:1019	arg1	ROS					963:965	intracellular ROS	949:965	intracellular ROS	949:965	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	3	67	theme	HaCaT	606:610	arg1	keratinocytes					612:624	human HaCaT keratinocytes	600:624	human HaCaT keratinocytes	600:624	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	7	68	theme	DNA	968:970	arg1	ROS					963:965	intracellular ROS	949:965	intracellular ROS	949:965	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	7	68	theme	DNA	968:970	arg1	damage					972:977	DNA damage	968:977	DNA damage	968:977	Exposure of UVB increased intracellular ROS, DNA damage, loss of mitochondrial membrane potential, apoptotic body formation causing cell death through the mitochondria-mediated apoptosis pathway.					
32599241	3	69	theme	fucoidan	489:496	arg1	SSQC1-SSQC4					509:519	SSQC1-SSQC4	509:519	SSQC1-SSQC4	509:519	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	69	theme	fucoidan	489:496	arg1	fractions					498:506	refining fucoidan fractions	480:506	refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum	480:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	70	from	siliquastrum	537:548	arg1	SSQC1-SSQC4					509:519	SSQC1-SSQC4	509:519	SSQC1-SSQC4	509:519	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	70	from	siliquastrum	537:548	arg1	fractions					498:506	refining fucoidan fractions	480:506	refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum	480:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	70	from	siliquastrum	537:548	arg1	evaluation					554:563	evaluation	554:563	evaluation of their UVB-protective effects in human HaCaT keratinocytes	554:624	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	70	from	siliquastrum	537:548	arg1	use					434:436	the use	430:436	the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum	430:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	0	71	theme	molecular	64:72	arg1	weight					74:79	low molecular weight	60:79	low molecular weight fucoidan	60:88	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.					
32599241	3	72	theme	effects	589:595	arg1	evaluation					554:563	evaluation	554:563	evaluation of their UVB-protective effects in human HaCaT keratinocytes	554:624	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	72	theme	effects	589:595	arg1	use					434:436	the use	430:436	the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum	430:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	1	73	theme	oxidative	181:189	arg1	damage					191:196	oxidative damage	181:196	oxidative damage to outermost layers of skin causing suntans, sunburns	181:250	Ultraviolet B (UVB) can induce oxidative damage to outermost layers of skin causing suntans, sunburns, and, in severe cases, blisters leading to photoaging.					
32599241	3	74	theme	refining	480:487	arg1	SSQC1-SSQC4					509:519	SSQC1-SSQC4	509:519	SSQC1-SSQC4	509:519	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	3	74	theme	refining	480:487	arg1	fractions					498:506	refining fucoidan fractions	480:506	refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum	480:548	The present study discloses the use of step gradient ethanol precipitation in refining fucoidan fractions (SSQC1-SSQC4) from Sargassum siliquastrum and evaluation of their UVB-protective effects in human HaCaT keratinocytes.					
32599241	1	75	theme	skin	221:224	arg1	sunburns					243:250	sunburns	243:250	sunburns	243:250	Ultraviolet B (UVB) can induce oxidative damage to outermost layers of skin causing suntans, sunburns, and, in severe cases, blisters leading to photoaging.					
32599241	1	75	theme	skin	221:224	arg1	suntans					234:240	skin causing suntans	221:240	skin causing suntans	221:240	Ultraviolet B (UVB) can induce oxidative damage to outermost layers of skin causing suntans, sunburns, and, in severe cases, blisters leading to photoaging.					
32599241	0	76	theme	low	60:62	arg1	weight					74:79	low molecular weight	60:79	low molecular weight fucoidan	60:88	Step gradient alcohol precipitation for the purification of low molecular weight fucoidan from Sargassum siliquastrum and its UVB protective effects.					
32599241	9	77	theme	HO-1	1340:1343	arg1	generation					1345:1354	Nrf2 mediated HO-1 generation	1326:1354	Nrf2 mediated HO-1 generation	1326:1354	SSQC4 treatment enhanced cellular antioxidant defense by increasing Nrf2 mediated HO-1 generation, which was identified as the cause of observed bioactivities.					
32599241	8	78	dep	exposed	1235:1241	arg1	UVB					1231:1233	UVB	1231:1233	UVB	1231:1233	SSQC4 treatment could dose-dependently attenuate the ROS levels and suppress mitochondria-mediated apoptosis in UVB exposed keratinocytes.					
32599241	1	79	theme	Ultraviolet	150:160	arg1	UVB					165:167	UVB	165:167	UVB	165:167	Ultraviolet B (UVB) can induce oxidative damage to outermost layers of skin causing suntans, sunburns, and, in severe cases, blisters leading to photoaging.					
32599241	1	79	theme	Ultraviolet	150:160	arg1	B					162:162	Ultraviolet B	150:162	Ultraviolet B (UVB)	150:168	Ultraviolet B (UVB) can induce oxidative damage to outermost layers of skin causing suntans, sunburns, and, in severe cases, blisters leading to photoaging.					
32599241	1	80	theme	causing	226:232	arg1	sunburns					243:250	sunburns	243:250	sunburns	243:250	Ultraviolet B (UVB) can induce oxidative damage to outermost layers of skin causing suntans, sunburns, and, in severe cases, blisters leading to photoaging.					
32599241	1	80	theme	causing	226:232	arg1	suntans					234:240	skin causing suntans	221:240	skin causing suntans	221:240	Ultraviolet B (UVB) can induce oxidative damage to outermost layers of skin causing suntans, sunburns, and, in severe cases, blisters leading to photoaging.					
34112409	6	0	theme	short-chain	1043:1053	arg1	SCFAs					1068:1072	SCFAs	1068:1072	SCFAs	1068:1072	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	6	0	theme	short-chain	1043:1053	arg1	acids					1061:1065	short-chain fatty acids	1043:1065	short-chain fatty acids (SCFAs)	1043:1073	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	7	1	theme	microbes	1150:1157	arg1	growth					1140:1145	the growth	1136:1145	the growth of microbes producing the SCFAs such as Peptococcus, Roseburia and Butyricicoccus in the cecum of KM mice	1136:1251	Nevertheless, CGCP gavage for four weeks could also promote the growth of microbes producing the SCFAs such as Peptococcus, Roseburia and Butyricicoccus in the cecum of KM mice.					
34112409	2	2	theme	molecular	363:371	arg1	kDa					449:451	kDa	449:451	kDa	449:451	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	2	2	theme	molecular	363:371	arg1	weight					373:378	The molecular weight	359:378	The molecular weight of water extracted polysaccharide of G. chouae (WGCP)	359:432	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	5	3	theme	fermentation	708:719	arg1	24 h					700:703	24 h	700:703	24 h of fermentation in vitro	700:728	After 24 h of fermentation in vitro, the pH values of CGLP in the fecal culture decreased from 6.89 to 4.82, similar to the inulin but significantly (p < 0.05) lower than those of the WGCP and normal control.					
34112409	8	4	theme	acid-extraction	1314:1328	arg1	method					1330:1335	acid-extraction method	1314:1335	acid-extraction method	1314:1335	The present study suggests that polysaccharides prepared by acid-extraction method could potentially be used as a good source of prebiotics.					
34112409	0	5	theme	extraction	102:111	arg1	methods					113:119	two extraction methods	98:119	two extraction methods	98:119	Digestion & fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods in vitro and in vivo.					
34112409	7	6	theme	CGCP	1090:1093	arg1	gavage					1095:1100	CGCP gavage	1090:1100	CGCP gavage for four weeks	1090:1115	Nevertheless, CGCP gavage for four weeks could also promote the growth of microbes producing the SCFAs such as Peptococcus, Roseburia and Butyricicoccus in the cecum of KM mice.					
34112409	8	7	theme	present	1258:1264	arg1	study					1266:1270	The present study	1254:1270	The present study	1254:1270	The present study suggests that polysaccharides prepared by acid-extraction method could potentially be used as a good source of prebiotics.					
34112409	7	8	theme	KM	1245:1246	arg1	mice					1248:1251	KM mice	1245:1251	KM mice	1245:1251	Nevertheless, CGCP gavage for four weeks could also promote the growth of microbes producing the SCFAs such as Peptococcus, Roseburia and Butyricicoccus in the cecum of KM mice.					
34112409	5	9	dep	lower	854:858	arg1	p < 0.05					844:851	p < 0.05	844:851	p < 0.05	844:851	After 24 h of fermentation in vitro, the pH values of CGLP in the fecal culture decreased from 6.89 to 4.82, similar to the inulin but significantly (p < 0.05) lower than those of the WGCP and normal control.					
34112409	7	10	theme	mice	1248:1251	arg1	cecum					1236:1240	the cecum	1232:1240	the cecum of KM mice	1232:1251	Nevertheless, CGCP gavage for four weeks could also promote the growth of microbes producing the SCFAs such as Peptococcus, Roseburia and Butyricicoccus in the cecum of KM mice.					
34112409	2	11	theme	G.	417:418	arg1	WGCP					428:431	WGCP	428:431	WGCP	428:431	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	2	11	theme	G.	417:418	arg1	chouae					420:425	G. chouae	417:425	G. chouae (WGCP)	417:432	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	0	12	theme	polysaccharides	53:67	arg1	characteristics					25:39	fermentation characteristics	12:39	fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods	12:119	Digestion & fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods in vitro and in vivo.					
34112409	5	13	theme	CGLP	748:751	arg1	values					738:743	the pH values	731:743	the pH values of CGLP in the fecal culture	731:772	After 24 h of fermentation in vitro, the pH values of CGLP in the fecal culture decreased from 6.89 to 4.82, similar to the inulin but significantly (p < 0.05) lower than those of the WGCP and normal control.					
34112409	6	14	dep	compositions	1014:1025	arg1	the					999:1001	the	999:1001	the	999:1001	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	2	15	theme	polysaccharide	399:412	arg1	kDa					449:451	kDa	449:451	kDa	449:451	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	2	15	theme	polysaccharide	399:412	arg1	weight					373:378	The molecular weight	359:378	The molecular weight of water extracted polysaccharide of G. chouae (WGCP)	359:432	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	6	16	theme	fermentation	957:968	arg1	characteristics					970:984	similar microbial fermentation characteristics	939:984	similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs)	939:1073	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	2	17	theme	extracted	389:397	arg1	polysaccharide					399:412	water extracted polysaccharide	383:412	water extracted polysaccharide of G. chouae (WGCP)	383:432	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	2	18	theme	1.73 × 103	438:447	arg1	kDa					449:451	kDa	449:451	kDa	449:451	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	2	18	theme	1.73 × 103	438:447	arg1	weight					373:378	The molecular weight	359:378	The molecular weight of water extracted polysaccharide of G. chouae (WGCP)	359:432	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	1	19	theme	fermentation	294:305	arg1	characteristics					307:321	their digestion and fermentation characteristics	274:321	characteristics	307:321	The sulfated polysaccharides extracted from Gracilaria chouae using critic acid extraction and water extraction, respectively, and their digestion and fermentation characteristics were compared in vitro and in vivo.					
34112409	5	20	dep	WGCP	878:881	arg1	the					874:876	the	874:876	the	874:876	After 24 h of fermentation in vitro, the pH values of CGLP in the fecal culture decreased from 6.89 to 4.82, similar to the inulin but significantly (p < 0.05) lower than those of the WGCP and normal control.					
34112409	1	21	theme	critic	211:216	arg1	extraction					223:232	critic acid extraction	211:232	critic acid extraction	211:232	The sulfated polysaccharides extracted from Gracilaria chouae using critic acid extraction and water extraction, respectively, and their digestion and fermentation characteristics were compared in vitro and in vivo.					
34112409	0	22	theme	fermentation	12:23	arg1	characteristics					25:39	fermentation characteristics	12:39	fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods	12:119	Digestion & fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods in vitro and in vivo.					
34112409	8	23	theme	prebiotics	1383:1392	arg1	polysaccharides					1286:1300	polysaccharides	1286:1300	polysaccharides prepared by acid-extraction method	1286:1335	The present study suggests that polysaccharides prepared by acid-extraction method could potentially be used as a good source of prebiotics.					
34112409	8	23	theme	prebiotics	1383:1392	arg1	source					1373:1378	a good source	1366:1378	a good source of prebiotics	1366:1392	The present study suggests that polysaccharides prepared by acid-extraction method could potentially be used as a good source of prebiotics.					
34112409	5	24	theme	normal	887:892	arg1	control					894:900	normal control	887:900	normal control	887:900	After 24 h of fermentation in vitro, the pH values of CGLP in the fecal culture decreased from 6.89 to 4.82, similar to the inulin but significantly (p < 0.05) lower than those of the WGCP and normal control.					
34112409	1	25	theme	acid	218:221	arg1	extraction					223:232	critic acid extraction	211:232	critic acid extraction	211:232	The sulfated polysaccharides extracted from Gracilaria chouae using critic acid extraction and water extraction, respectively, and their digestion and fermentation characteristics were compared in vitro and in vivo.					
34112409	0	26	theme	sulfated	44:51	arg1	polysaccharides					53:67	sulfated polysaccharides	44:67	sulfated polysaccharides from Gracilaria chouae using two extraction methods	44:119	Digestion & fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods in vitro and in vivo.					
34112409	6	27	theme	microbiome	1003:1012	arg1	compositions					1014:1025	microbiome compositions	1003:1025	microbiome compositions	1003:1025	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	4	28	theme	WGCP	645:648	arg1	GI					638:639	GI	638:639	GI	638:639	However, the glycemic index (GI) of WGCP and CGCP were 17.7 and 36.12, respectively.					
34112409	4	28	theme	WGCP	645:648	arg1	glycemic index					622:635	the glycemic index	618:635	the glycemic index (GI) of WGCP and CGCP	618:657	However, the glycemic index (GI) of WGCP and CGCP were 17.7 and 36.12, respectively.					
34112409	4	28	theme	WGCP	645:648	arg1	17.7					664:667	17.7	664:667	17.7	664:667	However, the glycemic index (GI) of WGCP and CGCP were 17.7 and 36.12, respectively.					
34112409	4	29	theme	CGCP	654:657	arg1	GI					638:639	GI	638:639	GI	638:639	However, the glycemic index (GI) of WGCP and CGCP were 17.7 and 36.12, respectively.					
34112409	4	29	theme	CGCP	654:657	arg1	glycemic index					622:635	the glycemic index	618:635	the glycemic index (GI) of WGCP and CGCP	618:657	However, the glycemic index (GI) of WGCP and CGCP were 17.7 and 36.12, respectively.					
34112409	4	29	theme	CGCP	654:657	arg1	17.7					664:667	17.7	664:667	17.7	664:667	However, the glycemic index (GI) of WGCP and CGCP were 17.7 and 36.12, respectively.					
34112409	0	30	from	chouae	85:90	arg1	characteristics					25:39	fermentation characteristics	12:39	fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods	12:119	Digestion & fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods in vitro and in vivo.					
34112409	0	30	from	chouae	85:90	arg1	polysaccharides					53:67	sulfated polysaccharides	44:67	sulfated polysaccharides from Gracilaria chouae using two extraction methods	44:119	Digestion & fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods in vitro and in vivo.					
34112409	2	31	theme	chouae	420:425	arg1	polysaccharide					399:412	water extracted polysaccharide	383:412	water extracted polysaccharide of G. chouae (WGCP)	383:432	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	5	32	theme	fecal	760:764	arg1	culture					766:772	the fecal culture	756:772	the fecal culture	756:772	After 24 h of fermentation in vitro, the pH values of CGLP in the fecal culture decreased from 6.89 to 4.82, similar to the inulin but significantly (p < 0.05) lower than those of the WGCP and normal control.					
34112409	6	33	theme	acids	1061:1065	arg1	compositions					1014:1025	microbiome compositions	1003:1025	microbiome compositions	1003:1025	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	6	33	theme	acids	1061:1065	arg1	contents					1031:1038	contents	1031:1038	contents	1031:1038	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	2	34	theme	critic	459:464	arg1	acid					466:469	critic acid	459:469	critic acid extracted polysaccharide (CGCP)	459:501	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	5	35	from	values	738:743	arg1	culture					766:772	the fecal culture	756:772	the fecal culture	756:772	After 24 h of fermentation in vitro, the pH values of CGLP in the fecal culture decreased from 6.89 to 4.82, similar to the inulin but significantly (p < 0.05) lower than those of the WGCP and normal control.					
34112409	8	36	used	used	1358:1361	arg2	source					1373:1378	a good source	1366:1378	a good source of prebiotics	1366:1392	The present study suggests that polysaccharides prepared by acid-extraction method could potentially be used as a good source of prebiotics.					
34112409	8	36	used	used	1358:1361	arg2	polysaccharides					1286:1300	polysaccharides	1286:1300	polysaccharides prepared by acid-extraction method	1286:1335	The present study suggests that polysaccharides prepared by acid-extraction method could potentially be used as a good source of prebiotics.					
34112409	1	37	theme	water	238:242	arg1	extraction					244:253	water extraction	238:253	water extraction	238:253	The sulfated polysaccharides extracted from Gracilaria chouae using critic acid extraction and water extraction, respectively, and their digestion and fermentation characteristics were compared in vitro and in vivo.					
34112409	2	38	dep	extracted	389:397	arg1	water					383:387	water	383:387	water	383:387	The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73 × 103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa.					
34112409	1	39	theme	sulfated	147:154	arg1	polysaccharides					156:170	The sulfated polysaccharides	143:170	The sulfated polysaccharides extracted from Gracilaria chouae using critic acid extraction and water extraction, respectively, and their digestion and fermentation characteristics	143:321	The sulfated polysaccharides extracted from Gracilaria chouae using critic acid extraction and water extraction, respectively, and their digestion and fermentation characteristics were compared in vitro and in vivo.					
34112409	6	40	theme	microbial	947:955	arg1	characteristics					970:984	similar microbial fermentation characteristics	939:984	similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs)	939:1073	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	6	41	theme	fatty	1055:1059	arg1	SCFAs					1068:1072	SCFAs	1068:1072	SCFAs	1068:1072	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	6	41	theme	fatty	1055:1059	arg1	acids					1061:1065	short-chain fatty acids	1043:1065	short-chain fatty acids (SCFAs)	1043:1073	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	3	42	theme	During stimulated	517:533	arg1	digestion					552:560	During stimulated gastrointestinal digestion	517:560	During stimulated gastrointestinal digestion	517:560	During stimulated gastrointestinal digestion in vitro, WGCP and CGCP were lightly degraded.					
34112409	6	43	theme	similar	939:945	arg1	characteristics					970:984	similar microbial fermentation characteristics	939:984	similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs)	939:1073	In addition, CGCP and inulin showed similar microbial fermentation characteristics according to the microbiome compositions and contents of short-chain fatty acids (SCFAs).					
34112409	5	44	theme	pH	735:736	arg1	values					738:743	the pH values	731:743	the pH values of CGLP in the fecal culture	731:772	After 24 h of fermentation in vitro, the pH values of CGLP in the fecal culture decreased from 6.89 to 4.82, similar to the inulin but significantly (p < 0.05) lower than those of the WGCP and normal control.					
34112409	0	45	from	characteristics	25:39	arg1	chouae					85:90	Gracilaria chouae	74:90	Gracilaria chouae using two extraction methods	74:119	Digestion & fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods in vitro and in vivo.					
34112409	8	46	theme	good	1368:1371	arg1	polysaccharides					1286:1300	polysaccharides	1286:1300	polysaccharides prepared by acid-extraction method	1286:1335	The present study suggests that polysaccharides prepared by acid-extraction method could potentially be used as a good source of prebiotics.					
34112409	8	46	theme	good	1368:1371	arg1	source					1373:1378	a good source	1366:1378	a good source of prebiotics	1366:1392	The present study suggests that polysaccharides prepared by acid-extraction method could potentially be used as a good source of prebiotics.					
34112409	3	47	theme	gastrointestinal	535:550	arg1	digestion					552:560	During stimulated gastrointestinal digestion	517:560	During stimulated gastrointestinal digestion	517:560	During stimulated gastrointestinal digestion in vitro, WGCP and CGCP were lightly degraded.					
33268179	6	0	theme	metabolism-related	916:933	arg1	expression					943:952	lipid metabolism-related protein expression	910:952	lipid metabolism-related protein expression	910:952	Moreover, PRG reduced HFD-induced increase of lipid metabolism-related protein expression, along with adipocyte size in white adipose tissue.					
33268179	2	1	theme	anti-obesity	316:327	arg1	activity					329:336	its anti-obesity activity	312:336	its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice	312:387	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	4	2	theme	PRG	538:540	arg1	treatment					542:550	PRG treatment	538:550	PRG treatment	538:550	Moreover, PRG treatment improved gut permeability by increasing tight junction protein expression and colon length shortening.					
33268179	0	3	theme	diet-induced	76:87	arg1	obesity					89:95	high fat diet-induced obesity	67:95	high fat diet-induced obesity	67:95	Polysaccharide fraction from greens of Raphanus sativus alleviates high fat diet-induced obesity.					
33268179	6	4	theme	lipid	910:914	arg1	expression					943:952	lipid metabolism-related protein expression	910:952	lipid metabolism-related protein expression	910:952	Moreover, PRG reduced HFD-induced increase of lipid metabolism-related protein expression, along with adipocyte size in white adipose tissue.					
33268179	7	5	theme	gut	1139:1141	arg1	microbiota					1143:1152	gut microbiota	1139:1152	gut microbiota	1139:1152	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	1	6	dep	Radish	98:103	arg1	Raphanus					106:113	Raphanus	106:113	Raphanus sativus	106:121	Radish (Raphanus sativus) greens are commonly used as a vegetable in Korea; however, their anti-obesity effect has not been reported yet.					
33268179	3	7	dep	gain	438:441	arg1	percentage					456:465	percentage	456:465	percentage	456:465	Supplementation with 4 mg/kg PRG reduced weight gain and body fat percentage, and regulated serum biomarkers against HFD-induced obesity.					
33268179	2	8	theme	polysaccharide	252:265	arg1	fraction					267:274	the polysaccharide fraction	248:274	the polysaccharide fraction of radish greens (PRG)	248:297	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	6	9	theme	adipose	990:996	arg1	tissue					998:1003	white adipose tissue	984:1003	white adipose tissue	984:1003	Moreover, PRG reduced HFD-induced increase of lipid metabolism-related protein expression, along with adipocyte size in white adipose tissue.					
33268179	6	10	theme	HFD-induced	886:896	arg1	increase					898:905	HFD-induced increase	886:905	HFD-induced increase of lipid metabolism-related protein expression	886:952	Moreover, PRG reduced HFD-induced increase of lipid metabolism-related protein expression, along with adipocyte size in white adipose tissue.					
33268179	7	11	theme	gut	1106:1108	arg1	function					1118:1125	gut barrier function	1106:1125	gut barrier function	1106:1125	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	4	12	theme	tight	592:596	arg1	expression					615:624	tight junction protein expression	592:624	tight junction protein expression	592:624	Moreover, PRG treatment improved gut permeability by increasing tight junction protein expression and colon length shortening.					
33268179	1	13	used	used	144:147	arg2	vegetable					154:162	vegetable	154:162	vegetable	154:162	Radish (Raphanus sativus) greens are commonly used as a vegetable in Korea; however, their anti-obesity effect has not been reported yet.					
33268179	1	13	used	used	144:147	arg2	greens					124:129	Radish (Raphanus sativus) greens	98:129	Radish (Raphanus sativus) greens	98:129	Radish (Raphanus sativus) greens are commonly used as a vegetable in Korea; however, their anti-obesity effect has not been reported yet.					
33268179	4	14	theme	colon	630:634	arg1	shortening					643:652	colon length shortening	630:652	colon length shortening	630:652	Moreover, PRG treatment improved gut permeability by increasing tight junction protein expression and colon length shortening.					
33268179	5	15	theme	PRG	781:783	arg1	supplementation					785:799	PRG supplementation	781:799	PRG supplementation	781:799	HFD intake increased the proportion of Firmicutes and decreased the proportion of Bacteroidetes and Verrucomicrobia; however, PRG supplementation maintained gut microbial composition to normal diet condition.					
33268179	7	16	theme	barrier	1110:1116	arg1	function					1118:1125	gut barrier function	1106:1125	gut barrier function	1106:1125	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	3	17	theme	serum	482:486	arg1	biomarkers					488:497	serum biomarkers	482:497	serum biomarkers against HFD-induced obesity	482:525	Supplementation with 4 mg/kg PRG reduced weight gain and body fat percentage, and regulated serum biomarkers against HFD-induced obesity.					
33268179	6	18	theme	protein	935:941	arg1	expression					943:952	lipid metabolism-related protein expression	910:952	lipid metabolism-related protein expression	910:952	Moreover, PRG reduced HFD-induced increase of lipid metabolism-related protein expression, along with adipocyte size in white adipose tissue.					
33268179	5	19	theme	Verrucomicrobia	755:769	arg1	proportion					723:732	the proportion	719:732	the proportion of Bacteroidetes and Verrucomicrobia	719:769	HFD intake increased the proportion of Firmicutes and decreased the proportion of Bacteroidetes and Verrucomicrobia; however, PRG supplementation maintained gut microbial composition to normal diet condition.					
33268179	2	20	theme	fat	346:348	arg1	diet					350:353	high fat diet	341:353	high fat diet (HFD)	341:359	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	2	20	theme	fat	346:348	arg1	HFD					356:358	HFD	356:358	HFD	356:358	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	0	21	theme	Polysaccharide	0:13	arg1	fraction					15:22	Polysaccharide fraction	0:22	Polysaccharide fraction from greens of Raphanus sativus	0:54	Polysaccharide fraction from greens of Raphanus sativus alleviates high fat diet-induced obesity.					
33268179	6	22	theme	white	984:988	arg1	tissue					998:1003	white adipose tissue	984:1003	white adipose tissue	984:1003	Moreover, PRG reduced HFD-induced increase of lipid metabolism-related protein expression, along with adipocyte size in white adipose tissue.					
33268179	2	23	theme	greens	286:291	arg1	fraction					267:274	the polysaccharide fraction	248:274	the polysaccharide fraction of radish greens (PRG)	248:297	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	7	24	contain	has	1065:1067	arg2	properties					1082:1091	anti-obesity properties	1069:1091	anti-obesity properties	1069:1091	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	7	24	contain	has	1065:1067	arg2	prebiotic					1054:1062	a potential prebiotic	1042:1062	a potential prebiotic	1042:1062	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	7	24	contain	has	1065:1067	arg1	PRG					1035:1037	PRG	1035:1037	PRG	1035:1037	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	2	25	theme	high	341:344	arg1	diet					350:353	high fat diet	341:353	high fat diet (HFD)	341:359	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	2	25	theme	high	341:344	arg1	HFD					356:358	HFD	356:358	HFD	356:358	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	5	26	theme	gut	812:814	arg1	composition					826:836	gut microbial composition	812:836	gut microbial composition to normal diet condition	812:861	HFD intake increased the proportion of Firmicutes and decreased the proportion of Bacteroidetes and Verrucomicrobia; however, PRG supplementation maintained gut microbial composition to normal diet condition.					
33268179	6	27	from	size	976:979	arg1	tissue					998:1003	white adipose tissue	984:1003	white adipose tissue	984:1003	Moreover, PRG reduced HFD-induced increase of lipid metabolism-related protein expression, along with adipocyte size in white adipose tissue.					
33268179	2	28	from	activity	329:336	arg1	mice					384:387	high fat diet (HFD)-induced obese C57BL/6J mice	341:387	high fat diet (HFD)-induced obese C57BL/6J mice	341:387	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	2	29	theme	radish	279:284	arg1	PRG					294:296	PRG	294:296	PRG	294:296	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	2	29	theme	radish	279:284	arg1	greens					286:291	radish greens	279:291	radish greens (PRG)	279:297	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	2	30	theme	obese	369:373	arg1	mice					384:387	high fat diet (HFD)-induced obese C57BL/6J mice	341:387	high fat diet (HFD)-induced obese C57BL/6J mice	341:387	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	1	31	theme	Radish	98:103	arg1	greens					124:129	Radish (Raphanus sativus) greens	98:129	Radish (Raphanus sativus) greens	98:129	Radish (Raphanus sativus) greens are commonly used as a vegetable in Korea; however, their anti-obesity effect has not been reported yet.					
33268179	1	31	theme	Radish	98:103	arg1	vegetable					154:162	vegetable	154:162	vegetable	154:162	Radish (Raphanus sativus) greens are commonly used as a vegetable in Korea; however, their anti-obesity effect has not been reported yet.					
33268179	2	32	theme	-induced	360:367	arg1	mice					384:387	high fat diet (HFD)-induced obese C57BL/6J mice	341:387	high fat diet (HFD)-induced obese C57BL/6J mice	341:387	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	0	33	theme	Raphanus	39:46	arg1	sativus					48:54	Raphanus sativus	39:54	Raphanus sativus	39:54	Polysaccharide fraction from greens of Raphanus sativus alleviates high fat diet-induced obesity.					
33268179	6	34	theme	adipocyte	966:974	arg1	size					976:979	adipocyte size	966:979	adipocyte size in white adipose tissue	966:1003	Moreover, PRG reduced HFD-induced increase of lipid metabolism-related protein expression, along with adipocyte size in white adipose tissue.					
33268179	4	35	theme	protein	607:613	arg1	expression					615:624	tight junction protein expression	592:624	tight junction protein expression	592:624	Moreover, PRG treatment improved gut permeability by increasing tight junction protein expression and colon length shortening.					
33268179	4	36	theme	length	636:641	arg1	shortening					643:652	colon length shortening	630:652	colon length shortening	630:652	Moreover, PRG treatment improved gut permeability by increasing tight junction protein expression and colon length shortening.					
33268179	5	37	theme	normal	841:846	arg1	condition					853:861	normal diet condition	841:861	normal diet condition	841:861	HFD intake increased the proportion of Firmicutes and decreased the proportion of Bacteroidetes and Verrucomicrobia; however, PRG supplementation maintained gut microbial composition to normal diet condition.					
33268179	7	38	theme	lipid	1169:1173	arg1	metabolism					1175:1184	lipid metabolism	1169:1184	lipid metabolism	1169:1184	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	5	39	theme	Firmicutes	694:703	arg1	proportion					680:689	the proportion	676:689	the proportion of Firmicutes	676:703	HFD intake increased the proportion of Firmicutes and decreased the proportion of Bacteroidetes and Verrucomicrobia; however, PRG supplementation maintained gut microbial composition to normal diet condition.					
33268179	5	40	theme	diet	848:851	arg1	condition					853:861	normal diet condition	841:861	normal diet condition	841:861	HFD intake increased the proportion of Firmicutes and decreased the proportion of Bacteroidetes and Verrucomicrobia; however, PRG supplementation maintained gut microbial composition to normal diet condition.					
33268179	1	41	theme	anti-obesity	189:200	arg1	effect					202:207	their anti-obesity effect	183:207	their anti-obesity effect	183:207	Radish (Raphanus sativus) greens are commonly used as a vegetable in Korea; however, their anti-obesity effect has not been reported yet.					
33268179	7	42	theme	anti-obesity	1069:1080	arg1	prebiotic					1054:1062	a potential prebiotic	1042:1062	a potential prebiotic	1042:1062	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	7	42	theme	anti-obesity	1069:1080	arg1	properties					1082:1091	anti-obesity properties	1069:1091	anti-obesity properties	1069:1091	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	7	43	theme	potential	1044:1052	arg1	prebiotic					1054:1062	a potential prebiotic	1042:1062	a potential prebiotic	1042:1062	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	7	43	theme	potential	1044:1052	arg1	properties					1082:1091	anti-obesity properties	1069:1091	anti-obesity properties	1069:1091	These results indicated that PRG as a potential prebiotic, has anti-obesity properties by improving gut barrier function, modulating gut microbiota and regulating lipid metabolism.					
33268179	5	44	theme	Bacteroidetes	737:749	arg1	proportion					723:732	the proportion	719:732	the proportion of Bacteroidetes and Verrucomicrobia	719:769	HFD intake increased the proportion of Firmicutes and decreased the proportion of Bacteroidetes and Verrucomicrobia; however, PRG supplementation maintained gut microbial composition to normal diet condition.					
33268179	0	45	theme	sativus	48:54	arg1	greens					29:34	greens	29:34	greens of Raphanus sativus	29:54	Polysaccharide fraction from greens of Raphanus sativus alleviates high fat diet-induced obesity.					
33268179	5	46	theme	HFD	655:657	arg1	intake					659:664	HFD intake	655:664	HFD intake	655:664	HFD intake increased the proportion of Firmicutes and decreased the proportion of Bacteroidetes and Verrucomicrobia; however, PRG supplementation maintained gut microbial composition to normal diet condition.					
33268179	3	47	theme	body	447:450	arg1	fat					452:454	body fat	447:454	body fat	447:454	Supplementation with 4 mg/kg PRG reduced weight gain and body fat percentage, and regulated serum biomarkers against HFD-induced obesity.					
33268179	6	48	theme	expression	943:952	arg1	increase					898:905	HFD-induced increase	886:905	HFD-induced increase of lipid metabolism-related protein expression	886:952	Moreover, PRG reduced HFD-induced increase of lipid metabolism-related protein expression, along with adipocyte size in white adipose tissue.					
33268179	3	49	theme	weight	431:436	arg1	gain					438:441	weight gain	431:441	weight gain	431:441	Supplementation with 4 mg/kg PRG reduced weight gain and body fat percentage, and regulated serum biomarkers against HFD-induced obesity.					
33268179	3	50	theme	HFD-induced	507:517	arg1	obesity					519:525	HFD-induced obesity	507:525	HFD-induced obesity	507:525	Supplementation with 4 mg/kg PRG reduced weight gain and body fat percentage, and regulated serum biomarkers against HFD-induced obesity.					
33268179	3	51	with	Supplementation	390:404	arg1	PRG					419:421	4 mg/kg PRG	411:421	4 mg/kg PRG	411:421	Supplementation with 4 mg/kg PRG reduced weight gain and body fat percentage, and regulated serum biomarkers against HFD-induced obesity.					
33268179	5	52	theme	microbial	816:824	arg1	composition					826:836	gut microbial composition	812:836	gut microbial composition to normal diet condition	812:861	HFD intake increased the proportion of Firmicutes and decreased the proportion of Bacteroidetes and Verrucomicrobia; however, PRG supplementation maintained gut microbial composition to normal diet condition.					
33268179	4	53	theme	gut	561:563	arg1	permeability					565:576	gut permeability	561:576	gut permeability	561:576	Moreover, PRG treatment improved gut permeability by increasing tight junction protein expression and colon length shortening.					
33268179	0	54	theme	fat	72:74	arg1	obesity					89:95	high fat diet-induced obesity	67:95	high fat diet-induced obesity	67:95	Polysaccharide fraction from greens of Raphanus sativus alleviates high fat diet-induced obesity.					
33268179	3	55	theme	4 mg/kg	411:417	arg1	PRG					419:421	4 mg/kg PRG	411:421	4 mg/kg PRG	411:421	Supplementation with 4 mg/kg PRG reduced weight gain and body fat percentage, and regulated serum biomarkers against HFD-induced obesity.					
33268179	4	56	theme	junction	598:605	arg1	expression					615:624	tight junction protein expression	592:624	tight junction protein expression	592:624	Moreover, PRG treatment improved gut permeability by increasing tight junction protein expression and colon length shortening.					
33268179	0	57	theme	high	67:70	arg1	obesity					89:95	high fat diet-induced obesity	67:95	high fat diet-induced obesity	67:95	Polysaccharide fraction from greens of Raphanus sativus alleviates high fat diet-induced obesity.					
33268179	0	58	from	greens	29:34	arg1	fraction					15:22	Polysaccharide fraction	0:22	Polysaccharide fraction from greens of Raphanus sativus	0:54	Polysaccharide fraction from greens of Raphanus sativus alleviates high fat diet-induced obesity.					
33268179	2	59	theme	C57BL/6J	375:382	arg1	mice					384:387	high fat diet (HFD)-induced obese C57BL/6J mice	341:387	high fat diet (HFD)-induced obese C57BL/6J mice	341:387	We prepared the polysaccharide fraction of radish greens (PRG) and assessed its anti-obesity activity in high fat diet (HFD)-induced obese C57BL/6J mice.					
33268179	1	60	dep	Raphanus	106:113	arg1	sativus					115:121	sativus	115:121	Raphanus sativus	106:121	Radish (Raphanus sativus) greens are commonly used as a vegetable in Korea; however, their anti-obesity effect has not been reported yet.					
32170892	2	0	theme	scaffolds	331:339	arg1	porous-structure					294:309	The 3D porous-structure	287:309	The 3D porous-structure of the biocomposite scaffolds	287:339	The 3D porous-structure of the biocomposite scaffolds were confirmed by Scanning Electron Microscopy and Microtomography analysis.					
32170892	4	1	theme	matrices	575:582	arg1	properties					557:566	The hydrogel properties	544:566	The hydrogel properties of the matrices	544:582	The hydrogel properties of the matrices were studied by swelling and mechanical tests, indicating that the scaffold presented a good mechanical behavior.					
32170892	7	2	theme	cytotoxic	1079:1087	arg1	effect					1089:1094	no cytotoxic effect	1076:1094	no cytotoxic effect	1076:1094	On the other hand, no cytotoxic effect was observed when RAW264.7 cells were seeded on the scaffolds.					
32170892	3	3	theme	composite	509:517	arg1	components					491:500	the organic components	479:500	the organic components of the composite and nHA in the scaffold	479:541	Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold.					
32170892	3	4	from	scaffold	534:541	arg1	components					491:500	the organic components	479:500	the organic components of the composite and nHA in the scaffold	479:541	Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold.					
32170892	6	5	theme	progenitor	916:925	arg1	cells					927:931	mesenchymal progenitor cells	904:931	bone marrow mesenchymal progenitor cells (BMPC)	892:938	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	6	5	theme	progenitor	916:925	arg1	BMPC					934:937	BMPC	934:937	BMPC	934:937	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	3	6	from	composite	509:517	arg1	scaffold					534:541	the scaffold	530:541	the scaffold	530:541	Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold.					
32170892	1	7	theme	ultrasonic	230:239	arg1	methodology					241:251	ultrasonic methodology	230:251	ultrasonic methodology	230:251	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	6	8	theme	mesenchymal	904:914	arg1	cells					927:931	mesenchymal progenitor cells	904:931	bone marrow mesenchymal progenitor cells (BMPC)	892:938	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	6	8	theme	mesenchymal	904:914	arg1	BMPC					934:937	BMPC	934:937	BMPC	934:937	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	7	9	theme	RAW264.7	1114:1121	arg1	cells					1123:1127	RAW264.7 cells	1114:1127	RAW264.7 cells	1114:1127	On the other hand, no cytotoxic effect was observed when RAW264.7 cells were seeded on the scaffolds.					
32170892	3	10	theme	organic	483:489	arg1	components					491:500	the organic components	479:500	the organic components of the composite and nHA in the scaffold	479:541	Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold.					
32170892	3	11	from	nHA	523:525	arg1	scaffold					534:541	the scaffold	530:541	the scaffold	530:541	Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold.					
32170892	2	12	theme	Scanning	359:366	arg1	Microscopy					377:386	Scanning Electron Microscopy	359:386	Scanning Electron Microscopy	359:386	The 3D porous-structure of the biocomposite scaffolds were confirmed by Scanning Electron Microscopy and Microtomography analysis.					
32170892	8	13	theme	promising	1237:1245	arg1	candidates					1247:1256	promising candidates	1237:1256	promising candidates	1237:1256	Altogether, our results allow us to conclude that these nanobiocomposites are promising candidates to induce bone tissue regeneration.					
32170892	8	13	theme	promising	1237:1245	arg1	nanobiocomposites					1215:1231	these nanobiocomposites	1209:1231	these nanobiocomposites	1209:1231	Altogether, our results allow us to conclude that these nanobiocomposites are promising candidates to induce bone tissue regeneration.					
32170892	5	14	theme	nHA	792:794	arg1	addition					780:787	the addition	776:787	the addition of nHA	776:794	The degradation test demonstrated that the material is slowly degraded, while the addition of nHA slightly influences the degradation of the scaffolds.					
32170892	6	15	theme	bone	892:895	arg1	marrow					897:902	bone marrow mesenchymal progenitor cells (BMPC)	892:938	bone marrow mesenchymal progenitor cells (BMPC)	892:938	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	1	16	with	cellulose	144:152	arg1	nHA					186:188	nHA	186:188	nHA	186:188	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	1	16	with	cellulose	144:152	arg1	hydroxyapatite					170:183	nanometric hydroxyapatite	159:183	nanometric hydroxyapatite (nHA)	159:189	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	6	17	theme	Biocompatibility	850:865	arg1	studies					867:873	Biocompatibility studies	850:873	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC)	850:938	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	2	18	theme	Electron	368:375	arg1	Microscopy					377:386	Scanning Electron Microscopy	359:386	Scanning Electron Microscopy	359:386	The 3D porous-structure of the biocomposite scaffolds were confirmed by Scanning Electron Microscopy and Microtomography analysis.					
32170892	3	19	theme	nHA	523:525	arg1	components					491:500	the organic components	479:500	the organic components of the composite and nHA in the scaffold	479:541	Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold.					
32170892	1	20	with	chitosan	117:124	arg1	nHA					186:188	nHA	186:188	nHA	186:188	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	1	20	with	chitosan	117:124	arg1	hydroxyapatite					170:183	nanometric hydroxyapatite	159:183	nanometric hydroxyapatite (nHA)	159:189	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	6	21	theme	phosphatase	984:994	arg1	activity					996:1003	alkaline phosphatase activity	975:1003	alkaline phosphatase activity	975:1003	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	1	22	theme	composite	89:97	arg1	hydrogel					99:106	a composite hydrogel	87:106	a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%),	87:225	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	3	23	theme	Infrared	418:425	arg1	analysis					427:434	Infrared analysis	418:434	Infrared analysis	418:434	Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold.					
32170892	4	24	theme	hydrogel	548:555	arg1	properties					557:566	The hydrogel properties	544:566	The hydrogel properties of the matrices	544:582	The hydrogel properties of the matrices were studied by swelling and mechanical tests, indicating that the scaffold presented a good mechanical behavior.					
32170892	3	25	from	components	491:500	arg1	scaffold					534:541	the scaffold	530:541	the scaffold	530:541	Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold.					
32170892	3	26	dep	composite	509:517	arg1	the					505:507	the	505:507	the	505:507	Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold.					
32170892	0	27	theme	natural	26:32	arg1	polyelectrolytes					34:49	natural polyelectrolytes	26:49	natural polyelectrolytes for bone regeneration	26:71	Nanobiocomposite based on natural polyelectrolytes for bone regeneration.					
32170892	6	28	theme	alkaline	975:982	arg1	phosphatase					984:994	alkaline phosphatase	975:994	alkaline phosphatase activity	975:1003	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	8	29	theme	bone	1268:1271	arg1	regeneration					1280:1291	bone tissue regeneration	1268:1291	bone tissue regeneration	1268:1291	Altogether, our results allow us to conclude that these nanobiocomposites are promising candidates to induce bone tissue regeneration.					
32170892	5	30	theme	degradation	702:712	arg1	test					714:717	The degradation test	698:717	The degradation test	698:717	The degradation test demonstrated that the material is slowly degraded, while the addition of nHA slightly influences the degradation of the scaffolds.					
32170892	2	31	theme	biocomposite	318:329	arg1	scaffolds					331:339	the biocomposite scaffolds	314:339	the biocomposite scaffolds	314:339	The 3D porous-structure of the biocomposite scaffolds were confirmed by Scanning Electron Microscopy and Microtomography analysis.					
32170892	7	32	theme	other	1064:1068	arg1	hand					1070:1073	the other hand	1060:1073	the other hand	1060:1073	On the other hand, no cytotoxic effect was observed when RAW264.7 cells were seeded on the scaffolds.					
32170892	5	33	theme	scaffolds	839:847	arg1	degradation					820:830	the degradation	816:830	the degradation of the scaffolds	816:847	The degradation test demonstrated that the material is slowly degraded, while the addition of nHA slightly influences the degradation of the scaffolds.					
32170892	2	34	theme	Microtomography	392:406	arg1	analysis					408:415	Microtomography analysis	392:415	Microtomography analysis	392:415	The 3D porous-structure of the biocomposite scaffolds were confirmed by Scanning Electron Microscopy and Microtomography analysis.					
32170892	1	35	theme	bone	268:271	arg1	regeneration					273:284	bone regeneration	268:284	bone regeneration	268:284	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	3	36	theme	specific	449:456	arg1	interactions					458:469	specific interactions	449:469	specific interactions between the organic components of the composite and nHA in the scaffold	449:541	Infrared analysis did not show specific interactions between the organic components of the composite and nHA in the scaffold.					
32170892	6	37	theme	cell	952:955	arg1	proliferation					957:969	cell proliferation	952:969	cell proliferation	952:969	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	1	38	dep	5	222:222	arg1	to					219:220	to	219:220	to	219:220	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	0	39	theme	bone	55:58	arg1	regeneration					60:71	bone regeneration	55:71	bone regeneration	55:71	Nanobiocomposite based on natural polyelectrolytes for bone regeneration.					
32170892	7	40	located	observed	1100:1107	arg1	hand					1070:1073	the other hand	1060:1073	the other hand	1060:1073	On the other hand, no cytotoxic effect was observed when RAW264.7 cells were seeded on the scaffolds.					
32170892	7	40	located	observed	1100:1107	arg2	effect					1089:1094	no cytotoxic effect	1076:1094	no cytotoxic effect	1076:1094	On the other hand, no cytotoxic effect was observed when RAW264.7 cells were seeded on the scaffolds.					
32170892	4	41	theme	good	672:675	arg1	behavior					688:695	a good mechanical behavior	670:695	a good mechanical behavior	670:695	The hydrogel properties of the matrices were studied by swelling and mechanical tests, indicating that the scaffold presented a good mechanical behavior.					
32170892	1	42	theme	carboxymethyl	130:142	arg1	cellulose					144:152	carboxymethyl cellulose	130:152	carboxymethyl cellulose	130:152	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	6	43	theme	nHA	1044:1046	arg1	content					1048:1054	the matrix nHA content	1033:1054	the matrix nHA content	1033:1054	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	2	44	theme	3D	291:292	arg1	porous-structure					294:309	The 3D porous-structure	287:309	The 3D porous-structure of the biocomposite scaffolds	287:339	The 3D porous-structure of the biocomposite scaffolds were confirmed by Scanning Electron Microscopy and Microtomography analysis.					
32170892	6	45	theme	matrix	1037:1042	arg1	content					1048:1054	the matrix nHA content	1033:1054	the matrix nHA content	1033:1054	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	8	46	theme	tissue	1273:1278	arg1	regeneration					1280:1291	bone tissue regeneration	1268:1291	bone tissue regeneration	1268:1291	Altogether, our results allow us to conclude that these nanobiocomposites are promising candidates to induce bone tissue regeneration.					
32170892	4	47	theme	mechanical	677:686	arg1	behavior					688:695	a good mechanical behavior	670:695	a good mechanical behavior	670:695	The hydrogel properties of the matrices were studied by swelling and mechanical tests, indicating that the scaffold presented a good mechanical behavior.					
32170892	4	48	theme	mechanical	613:622	arg1	tests					624:628	mechanical tests	613:628	mechanical tests	613:628	The hydrogel properties of the matrices were studied by swelling and mechanical tests, indicating that the scaffold presented a good mechanical behavior.					
32170892	1	49	theme	nanometric	159:168	arg1	nHA					186:188	nHA	186:188	nHA	186:188	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	1	49	theme	nanometric	159:168	arg1	hydroxyapatite					170:183	nanometric hydroxyapatite	159:183	nanometric hydroxyapatite (nHA)	159:189	We developed a composite hydrogel based on chitosan and carboxymethyl cellulose with nanometric hydroxyapatite (nHA) as filler (ranging from 0.5 to 5%), by ultrasonic methodology to be used for bone regeneration.					
32170892	6	50	dep	marrow	897:902	arg1	cells					927:931	mesenchymal progenitor cells	904:931	bone marrow mesenchymal progenitor cells (BMPC)	892:938	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
32170892	6	50	dep	marrow	897:902	arg1	BMPC					934:937	BMPC	934:937	BMPC	934:937	Biocompatibility studies carried out with bone marrow mesenchymal progenitor cells (BMPC) showed that cell proliferation and alkaline phosphatase activity were increased depending on the matrix nHA content.					
33545860	3	0	theme	silk-fibroin	741:752	arg1	solutions					759:767	regenerated silk-fibroin (SF) solutions	729:767	regenerated silk-fibroin (SF) solutions	729:767	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	6	1	theme	polymeric	1376:1384	arg1	ratios					1386:1391	HA20/SF80 polymeric ratios	1366:1391	HA20/SF80 polymeric ratios	1366:1391	We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios.					
33545860	6	2	with	hydrogels	1351:1359	arg1	ratios					1386:1391	HA20/SF80 polymeric ratios	1366:1391	HA20/SF80 polymeric ratios	1366:1391	We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios.					
33545860	7	3	theme	chondrogenic	1559:1570	arg1	medium					1572:1577	chondrogenic medium	1559:1577	chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study	1559:1683	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	9	4	theme	tissue	2061:2066	arg1	engineering					2068:2078	cartilage tissue engineering	2051:2078	cartilage tissue engineering	2051:2078	In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.					
33545860	3	5	theme	injectable	577:586	arg1	hydrogels					588:596	injectable hydrogels	577:596	injectable hydrogels for drug delivery and cartilage tissue engineering	577:647	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	5	6	link	crosslinked	1028:1038	arg1	composites					1050:1059	enzymatically crosslinked HA-Tyr/SF composites	1014:1059	enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF)	1014:1098	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	7	7	theme	superior	1599:1606	arg1	production					1608:1617	the superior production	1595:1617	the superior production of ECM in this hydrogel	1595:1641	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	7	8	theme	culture	1548:1554	arg1	28 days					1537:1543	28 days	1537:1543	28 days of culture	1537:1554	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	5	9	theme	properties	924:933	arg1	effect					884:889	the effect	880:889	the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition	880:974	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	6	10	theme	proteins	1241:1248	arg1	expression					1210:1219	the expression	1206:1219	the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios	1206:1391	We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios.					
33545860	1	11	theme	tissue	216:221	arg1	homeostasis					223:233	tissue homeostasis	216:233	tissue homeostasis	216:233	Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes.					
33545860	6	12	theme	cartilage	1224:1232	arg1	proteins					1241:1248	cartilage matrix proteins	1224:1248	cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios	1224:1391	We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios.					
33545860	0	13	theme	drugs	154:158	arg1	delivery					111:118	delivery	111:118	delivery	111:118	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.					
33545860	0	13	theme	drugs	154:158	arg1	engineering					95:105	cartilage tissue engineering	78:105	cartilage tissue engineering	78:105	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.					
33545860	8	14	theme	long	1876:1879	arg1	duration					1891:1898	the long treatment duration	1872:1898	the long treatment duration typically necessary for osteoarthritic joints	1872:1944	Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints.					
33545860	7	15	from	production	1608:1617	arg1	hydrogel					1634:1641	this hydrogel	1629:1641	this hydrogel	1629:1641	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	5	16	theme	compositions	907:918	arg1	effect					884:889	the effect	880:889	the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition	880:974	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	6	17	theme	prominent	1303:1311	arg1	effects					1326:1332	the most prominent chondrogenic effects	1294:1332	the most prominent chondrogenic effects	1294:1332	We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios.					
33545860	5	18	theme	HA-Tyr	1103:1108	arg1	hydrogels					1110:1118	HA-Tyr hydrogels	1103:1118	HA-Tyr hydrogels	1103:1118	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	3	19	theme	cartilage	620:628	arg1	engineering					637:647	cartilage tissue engineering	620:647	cartilage tissue engineering	620:647	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	8	20	theme	necessary	1910:1918	arg1	duration					1891:1898	the long treatment duration	1872:1898	the long treatment duration typically necessary for osteoarthritic joints	1872:1944	Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints.					
33545860	7	21	theme	HA20/SF80	1464:1472	arg1	constructs					1492:1501	HA20/SF80 chondrocyte-laden constructs	1464:1501	HA20/SF80 chondrocyte-laden constructs	1464:1501	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	4	22	theme	enzymatic	775:783	arg1	crosslinking					785:796	enzymatic crosslinking	775:796	enzymatic crosslinking	775:796	Upon enzymatic crosslinking, the gelation and mechanical properties were characterized over time.					
33545860	8	23	theme	osteoarthritic	1924:1937	arg1	joints					1939:1944	osteoarthritic joints	1924:1944	osteoarthritic joints	1924:1944	Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints.					
33545860	0	24	theme	tissue	88:93	arg1	engineering					95:105	cartilage tissue engineering	78:105	cartilage tissue engineering	78:105	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.					
33545860	1	25	theme	cartilage	181:189	arg1	Injury					161:166	Injury	161:166	Injury of articular cartilage	161:189	Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes.					
33545860	5	26	from	hydrogels	1110:1118	arg1	work					1073:1076	further work	1065:1076	further work abbreviated as HA/SF	1065:1097	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	5	26	from	hydrogels	1110:1118	arg1	embedded					1002:1009	embedded	1002:1009	embedded	1002:1009	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	4	27	theme	mechanical	816:825	arg1	properties					827:836	mechanical properties	816:836	mechanical properties	816:836	Upon enzymatic crosslinking, the gelation and mechanical properties were characterized over time.					
33545860	5	28	theme	HA-Tyr/SF	1040:1048	arg1	composites					1050:1059	enzymatically crosslinked HA-Tyr/SF composites	1014:1059	enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF)	1014:1098	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	2	29	theme	tissue	431:436	arg1	engineering					438:448	cartilage tissue engineering	421:448	cartilage tissue engineering	421:448	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	7	30	theme	compressive	1440:1450	arg1	modulus					1452:1458	the compressive modulus	1436:1458	the compressive modulus for HA20/SF80 chondrocyte-laden constructs	1436:1501	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	1	31	theme	affected	295:302	arg1	tissue					304:309	the affected tissue	291:309	the affected tissue	291:309	Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes.					
33545860	0	32	theme	hyaluronic	16:25	arg1	hydrogels					64:72	hyaluronic acid-tyramine/silk-fibroin composite hydrogels	16:72	hyaluronic acid-tyramine/silk-fibroin composite hydrogels	16:72	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.					
33545860	1	33	theme	articular	171:179	arg1	cartilage					181:189	articular cartilage	171:189	articular cartilage	171:189	Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes.					
33545860	0	34	theme	composite	54:62	arg1	hydrogels					64:72	hyaluronic acid-tyramine/silk-fibroin composite hydrogels	16:72	hyaluronic acid-tyramine/silk-fibroin composite hydrogels	16:72	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.					
33545860	1	35	theme	osteoarthritic	326:339	arg1	changes					341:347	osteoarthritic changes	326:347	osteoarthritic changes	326:347	Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes.					
33545860	7	36	theme	other	1655:1659	arg1	hydrogels					1661:1669	other hydrogels	1655:1669	other hydrogels in this study	1655:1683	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	7	37	theme	Unconfined	1394:1403	arg1	testing					1416:1422	Unconfined mechanical testing	1394:1422	Unconfined mechanical testing	1394:1422	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	2	38	theme	desirable	494:502	arg1	hydrogels					407:415	injectable and highly tunable composite hydrogels	367:415	injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery	367:466	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	2	38	theme	desirable	494:502	arg1	alternative					504:514	a desirable alternative	492:514	a desirable alternative to invasive treatments	492:537	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	3	39	theme	hyaluronic	690:699	arg1	acid-tyramine					701:713	hyaluronic acid-tyramine	690:713	hyaluronic acid-tyramine (HA-Tyr)	690:722	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	3	39	theme	hyaluronic	690:699	arg1	HA-Tyr					716:721	HA-Tyr	716:721	HA-Tyr	716:721	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	2	40	theme	injectable	367:376	arg1	hydrogels					407:415	injectable and highly tunable composite hydrogels	367:415	injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery	367:466	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	2	40	theme	injectable	367:376	arg1	alternative					504:514	a desirable alternative	492:514	a desirable alternative to invasive treatments	492:537	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	3	41	theme	regenerated	729:739	arg1	solutions					759:767	regenerated silk-fibroin (SF) solutions	729:767	regenerated silk-fibroin (SF) solutions	729:767	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	5	42	theme	further	1065:1071	arg1	work					1073:1076	further work	1065:1076	further work abbreviated as HA/SF	1065:1097	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	9	43	theme	suitable	2015:2022	arg1	hydrogel					1972:1979	HA20/SF80 hydrogel	1962:1979	HA20/SF80 hydrogel	1962:1979	In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.					
33545860	9	43	theme	suitable	2015:2022	arg1	biomaterial					2035:2045	a suitable injectable biomaterial	2013:2045	a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications	2013:2109	In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.					
33545860	2	44	theme	composite	397:405	arg1	hydrogels					407:415	injectable and highly tunable composite hydrogels	367:415	injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery	367:466	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	2	44	theme	composite	397:405	arg1	alternative					504:514	a desirable alternative	492:514	a desirable alternative to invasive treatments	492:537	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	5	45	from	embedded	1002:1009	arg1	composites					1050:1059	enzymatically crosslinked HA-Tyr/SF composites	1014:1059	enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF)	1014:1098	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	5	45	from	embedded	1002:1009	arg1	hydrogels					1110:1118	HA-Tyr hydrogels	1103:1118	HA-Tyr hydrogels	1103:1118	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	1	46	theme	poor	251:254	arg1	capacity					269:276	the poor self-healing capacity	247:276	the poor self-healing capacity of cartilage	247:289	Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes.					
33545860	7	47	theme	ECM	1622:1624	arg1	production					1608:1617	the superior production	1595:1617	the superior production of ECM in this hydrogel	1595:1641	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	8	48	theme	anti-inflammatory	1737:1753	arg1	drugs					1755:1759	anti-inflammatory drugs	1737:1759	anti-inflammatory drugs	1737:1759	Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints.					
33545860	9	49	theme	cartilage	2051:2059	arg1	engineering					2068:2078	cartilage tissue engineering	2051:2078	cartilage tissue engineering	2051:2078	In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.					
33545860	8	50	theme	release	1823:1829	arg1	profile					1831:1837	the most sustained release profile	1804:1837	the most sustained release profile	1804:1837	Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints.					
33545860	1	51	theme	cartilage	281:289	arg1	capacity					269:276	the poor self-healing capacity	247:276	the poor self-healing capacity of cartilage	247:289	Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes.					
33545860	7	52	from	hydrogels	1661:1669	arg1	study					1679:1683	this study	1674:1683	this study	1674:1683	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	9	53	theme	drug	2084:2087	arg1	delivery					2089:2096	drug delivery	2084:2096	drug delivery	2084:2096	In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.					
33545860	3	54	theme	drug	602:605	arg1	delivery					607:614	drug delivery	602:614	drug delivery	602:614	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	9	55	dep	engineering	2068:2078	arg1	applications					2098:2109	applications	2098:2109	applications	2098:2109	In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.					
33545860	6	56	theme	matrix	1234:1239	arg1	proteins					1241:1248	cartilage matrix proteins	1224:1248	cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios	1224:1391	We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios.					
33545860	3	57	theme	tissue	630:635	arg1	engineering					637:647	cartilage tissue engineering	620:647	cartilage tissue engineering	620:647	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	5	58	theme	extracellular	938:950	arg1	ECM					960:962	ECM	960:962	ECM	960:962	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	5	58	theme	extracellular	938:950	arg1	matrix					952:957	extracellular matrix	938:957	extracellular matrix (ECM) deposition	938:974	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	5	59	theme	hydrogel	898:905	arg1	compositions					907:918	the hydrogel compositions	894:918	the hydrogel compositions	894:918	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	6	60	theme	chondrogenic	1313:1324	arg1	effects					1326:1332	the most prominent chondrogenic effects	1294:1332	the most prominent chondrogenic effects	1294:1332	We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios.					
33545860	8	61	theme	treatment	1881:1889	arg1	duration					1891:1898	the long treatment duration	1872:1898	the long treatment duration typically necessary for osteoarthritic joints	1872:1944	Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints.					
33545860	3	62	theme	different	662:670	arg1	concentrations					672:685	different concentrations	662:685	different concentrations of hyaluronic acid-tyramine (HA-Tyr)	662:722	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	5	63	theme	bovine	977:982	arg1	chondrocytes					984:995	bovine chondrocytes	977:995	bovine chondrocytes	977:995	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	7	64	theme	chondrocyte-laden	1474:1490	arg1	constructs					1492:1501	HA20/SF80 chondrocyte-laden constructs	1464:1501	HA20/SF80 chondrocyte-laden constructs	1464:1501	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	5	65	theme	matrix	952:957	arg1	deposition					965:974	extracellular matrix (ECM) deposition	938:974	extracellular matrix (ECM) deposition	938:974	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	8	66	dep	longest	1792:1798	arg1	the					1788:1790	the	1788:1790	the	1788:1790	Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints.					
33545860	1	67	from	imbalance	203:211	arg1	homeostasis					223:233	tissue homeostasis	216:233	tissue homeostasis	216:233	Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes.					
33545860	2	68	theme	invasive	519:526	arg1	treatments					528:537	invasive treatments	519:537	invasive treatments	519:537	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	0	69	theme	anti-inflammatory	123:139	arg1	drugs					154:158	anti-inflammatory and anabolic drugs	123:158	anti-inflammatory and anabolic drugs	123:158	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.					
33545860	5	70	theme	crosslinked	1028:1038	arg1	composites					1050:1059	enzymatically crosslinked HA-Tyr/SF composites	1014:1059	enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF)	1014:1098	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	0	71	theme	anabolic	145:152	arg1	drugs					154:158	anti-inflammatory and anabolic drugs	123:158	anti-inflammatory and anabolic drugs	123:158	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.					
33545860	5	72	from	effect	884:889	arg1	deposition					965:974	extracellular matrix (ECM) deposition	938:974	extracellular matrix (ECM) deposition	938:974	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	6	73	theme	hydrogel	1146:1153	arg1	formulations					1155:1166	all hydrogel formulations	1142:1166	all hydrogel formulations	1142:1166	We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios.					
33545860	2	74	theme	cartilage	421:429	arg1	engineering					438:448	cartilage tissue engineering	421:448	cartilage tissue engineering	421:448	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	6	75	located	observed	1339:1346	arg2	effects					1326:1332	the most prominent chondrogenic effects	1294:1332	the most prominent chondrogenic effects	1294:1332	We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios.					
33545860	6	75	located	observed	1339:1346	arg1	hydrogels					1351:1359	hydrogels	1351:1359	hydrogels with HA20/SF80 polymeric ratios	1351:1391	We demonstrated that all hydrogel formulations were cytocompatible and could promote the expression of cartilage matrix proteins allowing chondrocytes to produce ECM, while the most prominent chondrogenic effects were observed in hydrogels with HA20/SF80 polymeric ratios.					
33545860	0	76	theme	acid-tyramine/silk-fibroin	27:52	arg1	hydrogels					64:72	hyaluronic acid-tyramine/silk-fibroin composite hydrogels	16:72	hyaluronic acid-tyramine/silk-fibroin composite hydrogels	16:72	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.					
33545860	7	77	theme	mechanical	1405:1414	arg1	testing					1416:1422	Unconfined mechanical testing	1394:1422	Unconfined mechanical testing	1394:1422	Unconfined mechanical testing showed that the compressive modulus for HA20/SF80 chondrocyte-laden constructs was increased almost 10-fold over 28 days of culture in chondrogenic medium which confirmed the superior production of ECM in this hydrogel compared to other hydrogels in this study.					
33545860	3	78	theme	acid-tyramine	701:713	arg1	concentrations					672:685	different concentrations	662:685	different concentrations of hyaluronic acid-tyramine (HA-Tyr)	662:722	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	2	79	theme	drug	454:457	arg1	delivery					459:466	drug delivery	454:466	drug delivery	454:466	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	0	80	theme	hydrogels	64:72	arg1	Optimization					0:11	Optimization	0:11	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.	0:159	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.					
33545860	0	81	theme	cartilage	78:86	arg1	engineering					95:105	cartilage tissue engineering	78:105	cartilage tissue engineering	78:105	Optimization of hyaluronic acid-tyramine/silk-fibroin composite hydrogels for cartilage tissue engineering and delivery of anti-inflammatory and anabolic drugs.					
33545860	2	82	theme	recent	353:358	arg1	years					360:364	recent years	353:364	recent years	353:364	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	5	83	from	composites	1050:1059	arg1	work					1073:1076	further work	1065:1076	further work abbreviated as HA/SF	1065:1097	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	5	83	from	composites	1050:1059	arg1	embedded					1002:1009	embedded	1002:1009	embedded	1002:1009	To evaluate the effect of the hydrogel compositions and properties on extracellular matrix (ECM) deposition, bovine chondrocytes were embedded in enzymatically crosslinked HA-Tyr/SF composites (in further work abbreviated as HA/SF) or HA-Tyr hydrogels.					
33545860	9	84	theme	injectable	2024:2033	arg1	hydrogel					1972:1979	HA20/SF80 hydrogel	1962:1979	HA20/SF80 hydrogel	1962:1979	In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.					
33545860	9	84	theme	injectable	2024:2033	arg1	biomaterial					2035:2045	a suitable injectable biomaterial	2013:2045	a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications	2013:2109	In conclusion, HA20/SF80 hydrogel was successfully established as a suitable injectable biomaterial for cartilage tissue engineering and drug delivery applications.					
33545860	3	85	theme	SF	755:756	arg1	solutions					759:767	regenerated silk-fibroin (SF) solutions	729:767	regenerated silk-fibroin (SF) solutions	729:767	In this study, we aimed to formulate injectable hydrogels for drug delivery and cartilage tissue engineering by combining different concentrations of hyaluronic acid-tyramine (HA-Tyr) with regenerated silk-fibroin (SF) solutions.					
33545860	2	86	theme	tunable	389:395	arg1	hydrogels					407:415	injectable and highly tunable composite hydrogels	367:415	injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery	367:466	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	2	86	theme	tunable	389:395	arg1	alternative					504:514	a desirable alternative	492:514	a desirable alternative to invasive treatments	492:537	In recent years, injectable and highly tunable composite hydrogels for cartilage tissue engineering and drug delivery have been introduced as a desirable alternative to invasive treatments.					
33545860	8	87	theme	sustained	1813:1821	arg1	profile					1831:1837	the most sustained release profile	1804:1837	the most sustained release profile	1804:1837	Furthermore, in hydrogels loaded with anabolic and anti-inflammatory drugs, HA20/SF80 hydrogel showed the longest and the most sustained release profile over time which is desirable for the long treatment duration typically necessary for osteoarthritic joints.					
33545860	1	88	theme	self-healing	256:267	arg1	capacity					269:276	the poor self-healing capacity	247:276	the poor self-healing capacity of cartilage	247:289	Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due to the poor self-healing capacity of cartilage the affected tissue often exhibits osteoarthritic changes.					
33984612	7	0	theme	high	1146:1149	arg1	amounts					1160:1166	high curcumin amounts	1146:1166	high curcumin amounts	1146:1166	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	4	1	theme	xanthan	446:452	arg1	gum					454:456	xanthan gum	446:456	xanthan gum	446:456	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	6	2	theme	high	901:904	arg1	temperatures					906:917	high temperatures	901:917	high temperatures	901:917	Composite nanoparticles were coated by xanthan gum and remained stable over a wide pH range and at high temperatures and salt concentrations; they did not precipitate or aggregate after 30 days of storage.					
33984612	6	3	theme	salt	923:926	arg1	concentrations					928:941	salt concentrations	923:941	salt concentrations	923:941	Composite nanoparticles were coated by xanthan gum and remained stable over a wide pH range and at high temperatures and salt concentrations; they did not precipitate or aggregate after 30 days of storage.					
33984612	5	4	theme	zeta	608:611	arg1	potential					613:621	The zeta potential	604:621	The zeta potential	604:621	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	7	5	theme	xanthan	1034:1040	arg1	gum					1042:1044	xanthan gum	1034:1044	xanthan gum	1034:1044	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	6	6	theme	storage	999:1005	arg1	days					991:994	30 days	988:994	30 days of storage	988:1005	Composite nanoparticles were coated by xanthan gum and remained stable over a wide pH range and at high temperatures and salt concentrations; they did not precipitate or aggregate after 30 days of storage.					
33984612	2	7	from	pH	312:313	arg1	precipitation					295:307	anti-solvent precipitation	282:307	anti-solvent precipitation at pH 4.0	282:317	Zein-xanthan gum composite nanoparticles were prepared via anti-solvent precipitation at pH 4.0.					
33984612	3	8	theme	zeta	339:342	arg1	potential					344:352	zeta potential	339:352	zeta potential	339:352	The particle size, zeta potential, and stability of the system were related to the amount of xanthan gum added.					
33984612	6	9	theme	xanthan	841:847	arg1	gum					849:851	xanthan gum	841:851	xanthan gum	841:851	Composite nanoparticles were coated by xanthan gum and remained stable over a wide pH range and at high temperatures and salt concentrations; they did not precipitate or aggregate after 30 days of storage.					
33984612	8	10	theme	gum	1281:1283	arg1	nanoparticles					1285:1297	zein-xanthan gum nanoparticles	1268:1297	zein-xanthan gum nanoparticles	1268:1297	Therefore, zein-xanthan gum nanoparticles can be used for the delivery of biologically active compounds in food and pharmaceutical preparations.					
33984612	0	11	dep	in	94:95	arg1	vitro					97:101	vitro	97:101	vitro	97:101	Delivery of curcumin using a zein-xanthan gum nanocomplex: Fabrication, characterization, and in vitro release properties.					
33984612	4	12	theme	±	525:525	arg1	nm					531:532	179 ± 2.1 nm	521:532	179 ± 2.1 nm	521:532	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	12	theme	±	525:525	arg1	size					515:518	a small particle size	498:518	a small particle size (179 ± 2.1 nm)	498:533	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	5	13	theme	infrared	645:652	arg1	spectroscopy					654:665	infrared spectroscopy	645:665	infrared spectroscopy	645:665	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	5	14	theme	hydrogen	755:762	arg1	bonding					764:770	hydrogen bonding	755:770	hydrogen bonding	755:770	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	1	15	theme	xanthan	147:153	arg1	gum					155:157	xanthan gum	147:157	xanthan gum	147:157	This study aimed to use xanthan gum as a stabilizer to improve the stability of zein nanoparticles.					
33984612	1	15	theme	xanthan	147:153	arg1	stabilizer					164:173	a stabilizer	162:173	a stabilizer to improve the stability of zein nanoparticles	162:220	This study aimed to use xanthan gum as a stabilizer to improve the stability of zein nanoparticles.					
33984612	0	16	theme	in	94:95	arg1	properties					111:120	in vitro release properties	94:120	in vitro release properties	94:120	Delivery of curcumin using a zein-xanthan gum nanocomplex: Fabrication, characterization, and in vitro release properties.					
33984612	7	17	theme	nanoparticles	1121:1133	arg1	efficiency					1086:1095	encapsulation efficiency	1072:1095	encapsulation efficiency	1072:1095	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	7	17	theme	nanoparticles	1121:1133	arg1	capacity					1109:1116	loading capacity	1101:1116	loading capacity	1101:1116	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	4	18	theme	-42	575:577	arg1	±					579:579	±	579:579	±	579:579	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	5	19	theme	driving	728:734	arg1	force					736:740	the main driving force	719:740	the main driving force	719:740	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	5	19	theme	driving	728:734	arg1	attraction					704:713	electrostatic attraction	690:713	electrostatic attraction	690:713	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	5	20	theme	electrostatic	690:702	arg1	force					736:740	the main driving force	719:740	the main driving force	719:740	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	5	20	theme	electrostatic	690:702	arg1	attraction					704:713	electrostatic attraction	690:713	electrostatic attraction	690:713	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	7	21	theme	curcumin	1151:1158	arg1	amounts					1160:1166	high curcumin amounts	1146:1166	high curcumin amounts	1146:1166	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	7	22	from	release	1206:1212	arg1	fluid					1250:1254	simulated intestinal fluid	1229:1254	simulated intestinal fluid	1229:1254	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	3	23	theme	system	376:381	arg1	stability					359:367	stability	359:367	stability	359:367	The particle size, zeta potential, and stability of the system were related to the amount of xanthan gum added.					
33984612	3	23	theme	system	376:381	arg1	related					388:394	related	388:394	related	388:394	The particle size, zeta potential, and stability of the system were related to the amount of xanthan gum added.					
33984612	3	23	theme	system	376:381	arg1	size					333:336	particle size	324:336	particle size	324:336	The particle size, zeta potential, and stability of the system were related to the amount of xanthan gum added.					
33984612	3	23	theme	system	376:381	arg1	potential					344:352	zeta potential	339:352	zeta potential	339:352	The particle size, zeta potential, and stability of the system were related to the amount of xanthan gum added.					
33984612	7	24	dep	efficiency	1086:1095	arg1	the					1068:1070	the	1068:1070	the	1068:1070	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	7	25	theme	loading	1101:1107	arg1	capacity					1109:1116	loading capacity	1101:1116	loading capacity	1101:1116	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	4	26	theme	small	500:504	arg1	nm					531:532	179 ± 2.1 nm	521:532	179 ± 2.1 nm	521:532	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	26	theme	small	500:504	arg1	size					515:518	a small particle size	498:518	a small particle size (179 ± 2.1 nm)	498:533	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	27	with	nanoparticles	479:491	arg1	potential					564:572	sufficient negative zeta potential	539:572	sufficient negative zeta potential (-42 ± 1.6 mV)	539:587	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	27	with	nanoparticles	479:491	arg1	mV					585:586	-42 ± 1.6 mV	575:586	-42 ± 1.6 mV	575:586	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	27	with	nanoparticles	479:491	arg1	nm					531:532	179 ± 2.1 nm	521:532	179 ± 2.1 nm	521:532	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	27	with	nanoparticles	479:491	arg1	size					515:518	a small particle size	498:518	a small particle size (179 ± 2.1 nm)	498:533	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	28	theme	1.6	581:583	arg1	±					579:579	±	579:579	±	579:579	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	7	29	theme	encapsulation	1072:1084	arg1	efficiency					1086:1095	encapsulation efficiency	1072:1095	encapsulation efficiency	1072:1095	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	8	30	theme	pharmaceutical	1373:1386	arg1	preparations					1388:1399	pharmaceutical preparations	1373:1399	pharmaceutical preparations	1373:1399	Therefore, zein-xanthan gum nanoparticles can be used for the delivery of biologically active compounds in food and pharmaceutical preparations.					
33984612	4	31	theme	zeta	559:562	arg1	potential					564:572	sufficient negative zeta potential	539:572	sufficient negative zeta potential (-42 ± 1.6 mV)	539:587	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	31	theme	zeta	559:562	arg1	mV					585:586	-42 ± 1.6 mV	575:586	-42 ± 1.6 mV	575:586	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	0	32	theme	curcumin	12:19	arg1	Delivery					0:7	Delivery	0:7	Delivery of curcumin using a zein-xanthan gum nanocomplex: Fabrication, characterization, and in vitro release properties.	0:121	Delivery of curcumin using a zein-xanthan gum nanocomplex: Fabrication, characterization, and in vitro release properties.					
33984612	0	33	theme	release	103:109	arg1	properties					111:120	in vitro release properties	94:120	in vitro release properties	94:120	Delivery of curcumin using a zein-xanthan gum nanocomplex: Fabrication, characterization, and in vitro release properties.					
33984612	6	34	theme	Composite	802:810	arg1	nanoparticles					812:824	Composite nanoparticles	802:824	Composite nanoparticles	802:824	Composite nanoparticles were coated by xanthan gum and remained stable over a wide pH range and at high temperatures and salt concentrations; they did not precipitate or aggregate after 30 days of storage.					
33984612	2	35	theme	anti-solvent	282:293	arg1	precipitation					295:307	anti-solvent precipitation	282:307	anti-solvent precipitation at pH 4.0	282:317	Zein-xanthan gum composite nanoparticles were prepared via anti-solvent precipitation at pH 4.0.					
33984612	4	36	theme	negative	550:557	arg1	potential					564:572	sufficient negative zeta potential	539:572	sufficient negative zeta potential (-42 ± 1.6 mV)	539:587	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	36	theme	negative	550:557	arg1	mV					585:586	-42 ± 1.6 mV	575:586	-42 ± 1.6 mV	575:586	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	0	37	theme	zein-xanthan	29:40	arg1	nanocomplex					46:56	a zein-xanthan gum nanocomplex	27:56	a zein-xanthan gum nanocomplex	27:56	Delivery of curcumin using a zein-xanthan gum nanocomplex: Fabrication, characterization, and in vitro release properties.					
33984612	4	38	theme	particle	506:513	arg1	nm					531:532	179 ± 2.1 nm	521:532	179 ± 2.1 nm	521:532	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	38	theme	particle	506:513	arg1	size					515:518	a small particle size	498:518	a small particle size (179 ± 2.1 nm)	498:533	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	8	39	used	used	1306:1309	arg2	nanoparticles					1285:1297	zein-xanthan gum nanoparticles	1268:1297	zein-xanthan gum nanoparticles	1268:1297	Therefore, zein-xanthan gum nanoparticles can be used for the delivery of biologically active compounds in food and pharmaceutical preparations.					
33984612	6	40	theme	pH	885:886	arg1	range					888:892	a wide pH range	878:892	a wide pH range	878:892	Composite nanoparticles were coated by xanthan gum and remained stable over a wide pH range and at high temperatures and salt concentrations; they did not precipitate or aggregate after 30 days of storage.					
33984612	3	41	theme	xanthan	413:419	arg1	gum					421:423	xanthan gum	413:423	xanthan gum added	413:429	The particle size, zeta potential, and stability of the system were related to the amount of xanthan gum added.					
33984612	5	42	theme	hydrophobic	776:786	arg1	interactions					788:799	hydrophobic interactions	776:799	hydrophobic interactions	776:799	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	4	43	theme	spherical	469:477	arg1	nanoparticles					479:491	spherical nanoparticles	469:491	spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV)	469:587	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	44	theme	sufficient	539:548	arg1	potential					564:572	sufficient negative zeta potential	539:572	sufficient negative zeta potential (-42 ± 1.6 mV)	539:587	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	4	44	theme	sufficient	539:548	arg1	mV					585:586	-42 ± 1.6 mV	575:586	-42 ± 1.6 mV	575:586	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	3	45	theme	gum	421:423	arg1	amount					403:408	the amount	399:408	the amount of xanthan gum added	399:429	The particle size, zeta potential, and stability of the system were related to the amount of xanthan gum added.					
33984612	3	45	theme	gum	421:423	arg1	gum					421:423	xanthan gum	413:423	xanthan gum added	413:429	The particle size, zeta potential, and stability of the system were related to the amount of xanthan gum added.					
33984612	7	46	theme	gum	1042:1044	arg1	addition					1022:1029	the addition	1018:1029	the addition of xanthan gum	1018:1044	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	6	47	theme	wide	880:883	arg1	range					888:892	a wide pH range	878:892	a wide pH range	878:892	Composite nanoparticles were coated by xanthan gum and remained stable over a wide pH range and at high temperatures and salt concentrations; they did not precipitate or aggregate after 30 days of storage.					
33984612	5	48	theme	main	723:726	arg1	force					736:740	the main driving force	719:740	the main driving force	719:740	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	5	48	theme	main	723:726	arg1	attraction					704:713	electrostatic attraction	690:713	electrostatic attraction	690:713	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	8	49	theme	compounds	1351:1359	arg1	delivery					1319:1326	the delivery	1315:1326	the delivery of biologically active compounds in food and pharmaceutical preparations	1315:1399	Therefore, zein-xanthan gum nanoparticles can be used for the delivery of biologically active compounds in food and pharmaceutical preparations.					
33984612	0	50	theme	gum	42:44	arg1	nanocomplex					46:56	a zein-xanthan gum nanocomplex	27:56	a zein-xanthan gum nanocomplex	27:56	Delivery of curcumin using a zein-xanthan gum nanocomplex: Fabrication, characterization, and in vitro release properties.					
33984612	8	51	theme	active	1344:1349	arg1	compounds					1351:1359	biologically active compounds	1331:1359	biologically active compounds	1331:1359	Therefore, zein-xanthan gum nanoparticles can be used for the delivery of biologically active compounds in food and pharmaceutical preparations.					
33984612	7	52	contain	containing	1135:1144	arg1	nanoparticles					1121:1133	nanoparticles	1121:1133	nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid	1121:1254	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	7	52	contain	containing	1135:1144	arg2	amounts					1160:1166	high curcumin amounts	1146:1166	high curcumin amounts	1146:1166	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	7	53	theme	curcumin	1217:1224	arg1	release					1206:1212	slow and sustained release	1187:1212	slow and sustained release of curcumin in simulated intestinal fluid	1187:1254	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	5	54	dep	Fourier	627:633	arg1	transform					635:643	transform	635:643	transform infrared spectroscopy	635:665	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	2	55	theme	composite	240:248	arg1	nanoparticles					250:262	Zein-xanthan gum composite nanoparticles	223:262	Zein-xanthan gum composite nanoparticles	223:262	Zein-xanthan gum composite nanoparticles were prepared via anti-solvent precipitation at pH 4.0.					
33984612	7	56	theme	sustained	1196:1204	arg1	release					1206:1212	slow and sustained release	1187:1212	slow and sustained release of curcumin in simulated intestinal fluid	1187:1254	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	2	57	theme	gum	236:238	arg1	nanoparticles					250:262	Zein-xanthan gum composite nanoparticles	223:262	Zein-xanthan gum composite nanoparticles	223:262	Zein-xanthan gum composite nanoparticles were prepared via anti-solvent precipitation at pH 4.0.					
33984612	8	58	theme	zein-xanthan	1268:1279	arg1	nanoparticles					1285:1297	zein-xanthan gum nanoparticles	1268:1297	zein-xanthan gum nanoparticles	1268:1297	Therefore, zein-xanthan gum nanoparticles can be used for the delivery of biologically active compounds in food and pharmaceutical preparations.					
33984612	5	59	dep	potential	613:621	arg1	results					667:673	results	667:673	results	667:673	The zeta potential and Fourier transform infrared spectroscopy results indicated that electrostatic attraction was the main driving force, followed by hydrogen bonding and hydrophobic interactions.					
33984612	7	60	theme	simulated	1229:1237	arg1	fluid					1250:1254	simulated intestinal fluid	1229:1254	simulated intestinal fluid	1229:1254	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	2	61	theme	Zein-xanthan	223:234	arg1	nanoparticles					250:262	Zein-xanthan gum composite nanoparticles	223:262	Zein-xanthan gum composite nanoparticles	223:262	Zein-xanthan gum composite nanoparticles were prepared via anti-solvent precipitation at pH 4.0.					
33984612	7	62	theme	intestinal	1239:1248	arg1	fluid					1250:1254	simulated intestinal fluid	1229:1254	simulated intestinal fluid	1229:1254	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	7	63	theme	slow	1187:1190	arg1	release					1206:1212	slow and sustained release	1187:1212	slow and sustained release of curcumin in simulated intestinal fluid	1187:1254	Moreover, the addition of xanthan gum considerably improved the encapsulation efficiency and loading capacity of nanoparticles containing high curcumin amounts, which facilitated slow and sustained release of curcumin in simulated intestinal fluid.					
33984612	1	64	theme	zein	203:206	arg1	nanoparticles					208:220	zein nanoparticles	203:220	zein nanoparticles	203:220	This study aimed to use xanthan gum as a stabilizer to improve the stability of zein nanoparticles.					
33984612	3	65	theme	particle	324:331	arg1	size					333:336	particle size	324:336	particle size	324:336	The particle size, zeta potential, and stability of the system were related to the amount of xanthan gum added.					
33984612	3	65	theme	particle	324:331	arg1	related					388:394	related	388:394	related	388:394	The particle size, zeta potential, and stability of the system were related to the amount of xanthan gum added.					
33984612	4	66	theme	gum	454:456	arg1	mg					440:441	20 mg	437:441	20 mg of xanthan gum	437:456	When 20 mg of xanthan gum was added, spherical nanoparticles with a small particle size (179 ± 2.1 nm) and sufficient negative zeta potential (-42 ± 1.6 mV) were obtained.					
33984612	0	67	dep	Delivery	0:7	arg1	characterization					72:87	characterization	72:87	characterization	72:87	Delivery of curcumin using a zein-xanthan gum nanocomplex: Fabrication, characterization, and in vitro release properties.					
33984612	0	67	dep	Delivery	0:7	arg1	properties					111:120	in vitro release properties	94:120	in vitro release properties	94:120	Delivery of curcumin using a zein-xanthan gum nanocomplex: Fabrication, characterization, and in vitro release properties.					
33984612	0	67	dep	Delivery	0:7	arg1	Fabrication					59:69	Fabrication	59:69	Fabrication	59:69	Delivery of curcumin using a zein-xanthan gum nanocomplex: Fabrication, characterization, and in vitro release properties.					
33984612	8	68	from	delivery	1319:1326	arg1	food					1364:1367	food	1364:1367	food	1364:1367	Therefore, zein-xanthan gum nanoparticles can be used for the delivery of biologically active compounds in food and pharmaceutical preparations.					
33984612	8	68	from	delivery	1319:1326	arg1	preparations					1388:1399	pharmaceutical preparations	1373:1399	pharmaceutical preparations	1373:1399	Therefore, zein-xanthan gum nanoparticles can be used for the delivery of biologically active compounds in food and pharmaceutical preparations.					
33984612	1	69	theme	nanoparticles	208:220	arg1	stability					190:198	the stability	186:198	the stability of zein nanoparticles	186:220	This study aimed to use xanthan gum as a stabilizer to improve the stability of zein nanoparticles.					
33172614	0	0	theme	HepG2	99:103	arg1	cells					105:109	HepG2 cells	99:109	HepG2 cells	99:109	Water-soluble and alkali-soluble polysaccharides from bitter melon inhibited lipid accumulation in HepG2 cells and Caenorhabditis elegans.					
33172614	4	1	theme	monosaccharide	570:583	arg1	composition					585:595	monosaccharide composition	570:595	monosaccharide composition	570:595	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	1	theme	monosaccharide	570:583	arg1	contents					541:548	the uronic acid contents	525:548	the uronic acid contents (22.23% and 5.69%)	525:567	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	3	2	theme	alkali-soluble	332:345	arg1	polysaccharides					360:374	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides	307:374	polysaccharides	360:374	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides (ABPS) were extracted to evaluate the fat-lowering bioactivities in HepG2 cells and Caenorhabditis elegans.					
33172614	3	3	from	bioactivities	427:439	arg1	cells					450:454	HepG2 cells	444:454	HepG2 cells	444:454	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides (ABPS) were extracted to evaluate the fat-lowering bioactivities in HepG2 cells and Caenorhabditis elegans.					
33172614	3	3	from	bioactivities	427:439	arg1	elegans					475:481	Caenorhabditis elegans	460:481	Caenorhabditis elegans	460:481	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides (ABPS) were extracted to evaluate the fat-lowering bioactivities in HepG2 cells and Caenorhabditis elegans.					
33172614	6	4	theme	physical	1089:1096	arg1	activities					1098:1107	some physical activities	1084:1107	some physical activities	1084:1107	In the model of glucose-treated C. elegans, we observed that both WBPS and ABPS obviously suppressed the fat accumulation, more significantly by ABPS, along with no toxicity towards some physical activities.					
33172614	8	5	from	lowering	1328:1335	arg1	cells					1346:1350	HepG2 cells	1340:1350	HepG2 cells	1340:1350	Our studies demonstrated that both WBPS and ABPS can exhibit effects on fat- lowering in HepG2 cells and C. elegans.					
33172614	4	6	theme	uronic	529:534	arg1	composition					585:595	monosaccharide composition	570:595	monosaccharide composition	570:595	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	6	theme	uronic	529:534	arg1	weight					608:613	molecular weight	598:613	molecular weight (Mw: 332 kDa and 1552 kDa, respectively)	598:654	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	6	theme	uronic	529:534	arg1	contents					541:548	the uronic acid contents	525:548	the uronic acid contents (22.23% and 5.69%)	525:567	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	6	theme	uronic	529:534	arg1	spectra					663:669	IR spectra	660:669	IR spectra	660:669	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	7	7	theme	desaturases	1153:1163	arg1	pathways					1165:1172	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways	1110:1172	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways	1110:1172	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways were further confirmed to be involved in the lipid-lowering effects of BPSs.					
33172614	7	8	theme	acid	1148:1151	arg1	desaturases					1153:1163	fat-7 mediated fatty acid desaturases	1127:1163	fat-7 mediated fatty acid desaturases	1127:1163	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways were further confirmed to be involved in the lipid-lowering effects of BPSs.					
33172614	0	9	from	melon	61:65	arg1	polysaccharides					33:47	Water-soluble and alkali-soluble polysaccharides	0:47	Water-soluble and alkali-soluble polysaccharides from bitter melon	0:65	Water-soluble and alkali-soluble polysaccharides from bitter melon inhibited lipid accumulation in HepG2 cells and Caenorhabditis elegans.					
33172614	4	10	from	different	512:520	arg1	composition					585:595	monosaccharide composition	570:595	monosaccharide composition	570:595	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	10	from	different	512:520	arg1	weight					608:613	molecular weight	598:613	molecular weight (Mw: 332 kDa and 1552 kDa, respectively)	598:654	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	10	from	different	512:520	arg1	contents					541:548	the uronic acid contents	525:548	the uronic acid contents (22.23% and 5.69%)	525:567	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	10	from	different	512:520	arg1	spectra					663:669	IR spectra	660:669	IR spectra	660:669	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	0	11	theme	Caenorhabditis	115:128	arg1	elegans					130:136	Caenorhabditis elegans	115:136	Caenorhabditis elegans	115:136	Water-soluble and alkali-soluble polysaccharides from bitter melon inhibited lipid accumulation in HepG2 cells and Caenorhabditis elegans.					
33172614	5	12	theme	glucose	759:765	arg1	consumption					767:777	glucose consumption	759:777	glucose consumption	759:777	In palmitic acid-treated HepG2 cell, the ABPS exhibited better effects on accelerating glucose consumption and decreasing the triglyceride content than WBPS via stimulating glucose consumption (GLUT4) and gluconeogenesis (PEPCK).					
33172614	5	13	theme	triglyceride	798:809	arg1	content					811:817	the triglyceride content	794:817	the triglyceride content than WBPS	794:827	In palmitic acid-treated HepG2 cell, the ABPS exhibited better effects on accelerating glucose consumption and decreasing the triglyceride content than WBPS via stimulating glucose consumption (GLUT4) and gluconeogenesis (PEPCK).					
33172614	3	14	theme	Caenorhabditis	460:473	arg1	elegans					475:481	Caenorhabditis elegans	460:481	Caenorhabditis elegans	460:481	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides (ABPS) were extracted to evaluate the fat-lowering bioactivities in HepG2 cells and Caenorhabditis elegans.					
33172614	7	15	theme	mediated	1133:1140	arg1	desaturases					1153:1163	fat-7 mediated fatty acid desaturases	1127:1163	fat-7 mediated fatty acid desaturases	1127:1163	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways were further confirmed to be involved in the lipid-lowering effects of BPSs.					
33172614	5	16	theme	better	728:733	arg1	effects					735:741	better effects	728:741	better effects	728:741	In palmitic acid-treated HepG2 cell, the ABPS exhibited better effects on accelerating glucose consumption and decreasing the triglyceride content than WBPS via stimulating glucose consumption (GLUT4) and gluconeogenesis (PEPCK).					
33172614	3	17	theme	HepG2	444:448	arg1	cells					450:454	HepG2 cells	444:454	HepG2 cells	444:454	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides (ABPS) were extracted to evaluate the fat-lowering bioactivities in HepG2 cells and Caenorhabditis elegans.					
33172614	0	18	theme	Water-soluble	0:12	arg1	polysaccharides					33:47	Water-soluble and alkali-soluble polysaccharides	0:47	Water-soluble and alkali-soluble polysaccharides from bitter melon	0:65	Water-soluble and alkali-soluble polysaccharides from bitter melon inhibited lipid accumulation in HepG2 cells and Caenorhabditis elegans.					
33172614	4	19	dep	contents	541:548	arg1	%					556:556	22.23%	551:556	22.23%	551:556	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	19	dep	contents	541:548	arg1	%					566:566	5.69%	562:566	5.69%	562:566	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	8	20	theme	fat-	1323:1326	arg1	lowering					1328:1335	fat- lowering	1323:1335	fat- lowering in HepG2 cells	1323:1350	Our studies demonstrated that both WBPS and ABPS can exhibit effects on fat- lowering in HepG2 cells and C. elegans.					
33172614	5	21	theme	palmitic	675:682	arg1	cell					703:706	palmitic acid-treated HepG2 cell	675:706	palmitic acid-treated HepG2 cell	675:706	In palmitic acid-treated HepG2 cell, the ABPS exhibited better effects on accelerating glucose consumption and decreasing the triglyceride content than WBPS via stimulating glucose consumption (GLUT4) and gluconeogenesis (PEPCK).					
33172614	8	22	theme	HepG2	1340:1344	arg1	cells					1346:1350	HepG2 cells	1340:1350	HepG2 cells	1340:1350	Our studies demonstrated that both WBPS and ABPS can exhibit effects on fat- lowering in HepG2 cells and C. elegans.					
33172614	1	23	theme	hypolipidemic	201:213	arg1	effects					215:221	hypolipidemic effects	201:221	hypolipidemic effects	201:221	Bitter melon polysaccharides (BPS) have been reported to have hypolipidemic effects.					
33172614	0	24	theme	alkali-soluble	18:31	arg1	polysaccharides					33:47	Water-soluble and alkali-soluble polysaccharides	0:47	Water-soluble and alkali-soluble polysaccharides from bitter melon	0:65	Water-soluble and alkali-soluble polysaccharides from bitter melon inhibited lipid accumulation in HepG2 cells and Caenorhabditis elegans.					
33172614	5	25	theme	acid-treated	684:695	arg1	cell					703:706	palmitic acid-treated HepG2 cell	675:706	palmitic acid-treated HepG2 cell	675:706	In palmitic acid-treated HepG2 cell, the ABPS exhibited better effects on accelerating glucose consumption and decreasing the triglyceride content than WBPS via stimulating glucose consumption (GLUT4) and gluconeogenesis (PEPCK).					
33172614	1	26	contain	have	196:199	arg1	BPS					169:171	BPS	169:171	BPS	169:171	Bitter melon polysaccharides (BPS) have been reported to have hypolipidemic effects.					
33172614	1	26	contain	have	196:199	arg2	effects					215:221	hypolipidemic effects	201:221	hypolipidemic effects	201:221	Bitter melon polysaccharides (BPS) have been reported to have hypolipidemic effects.					
33172614	1	26	contain	have	196:199	arg1	polysaccharides					152:166	Bitter melon polysaccharides	139:166	Bitter melon polysaccharides (BPS)	139:172	Bitter melon polysaccharides (BPS) have been reported to have hypolipidemic effects.					
33172614	0	27	theme	bitter	54:59	arg1	melon					61:65	bitter melon	54:65	bitter melon	54:65	Water-soluble and alkali-soluble polysaccharides from bitter melon inhibited lipid accumulation in HepG2 cells and Caenorhabditis elegans.					
33172614	6	28	theme	elegans	937:943	arg1	model					909:913	the model	905:913	the model of glucose-treated C. elegans	905:943	In the model of glucose-treated C. elegans, we observed that both WBPS and ABPS obviously suppressed the fat accumulation, more significantly by ABPS, along with no toxicity towards some physical activities.					
33172614	5	29	theme	HepG2	697:701	arg1	cell					703:706	palmitic acid-treated HepG2 cell	675:706	palmitic acid-treated HepG2 cell	675:706	In palmitic acid-treated HepG2 cell, the ABPS exhibited better effects on accelerating glucose consumption and decreasing the triglyceride content than WBPS via stimulating glucose consumption (GLUT4) and gluconeogenesis (PEPCK).					
33172614	7	30	theme	fatty	1142:1146	arg1	desaturases					1153:1163	fat-7 mediated fatty acid desaturases	1127:1163	fat-7 mediated fatty acid desaturases	1127:1163	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways were further confirmed to be involved in the lipid-lowering effects of BPSs.					
33172614	7	31	theme	fat-6	1117:1121	arg1	pathways					1165:1172	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways	1110:1172	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways	1110:1172	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways were further confirmed to be involved in the lipid-lowering effects of BPSs.					
33172614	6	32	theme	C.	934:935	arg1	elegans					937:943	glucose-treated C. elegans	918:943	glucose-treated C. elegans	918:943	In the model of glucose-treated C. elegans, we observed that both WBPS and ABPS obviously suppressed the fat accumulation, more significantly by ABPS, along with no toxicity towards some physical activities.					
33172614	2	33	theme	precise	237:243	arg1	mechanism					245:253	the precise mechanism	233:253	the precise mechanism of BPS regulating lipid metabolism	233:288	However, the precise mechanism of BPS regulating lipid metabolism remains elusive.					
33172614	4	34	theme	acid	536:539	arg1	composition					585:595	monosaccharide composition	570:595	monosaccharide composition	570:595	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	34	theme	acid	536:539	arg1	weight					608:613	molecular weight	598:613	molecular weight (Mw: 332 kDa and 1552 kDa, respectively)	598:654	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	34	theme	acid	536:539	arg1	contents					541:548	the uronic acid contents	525:548	the uronic acid contents (22.23% and 5.69%)	525:567	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	34	theme	acid	536:539	arg1	spectra					663:669	IR spectra	660:669	IR spectra	660:669	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	5	35	theme	glucose	845:851	arg1	GLUT4					866:870	GLUT4	866:870	GLUT4	866:870	In palmitic acid-treated HepG2 cell, the ABPS exhibited better effects on accelerating glucose consumption and decreasing the triglyceride content than WBPS via stimulating glucose consumption (GLUT4) and gluconeogenesis (PEPCK).					
33172614	5	35	theme	glucose	845:851	arg1	consumption					853:863	glucose consumption	845:863	glucose consumption (GLUT4)	845:871	In palmitic acid-treated HepG2 cell, the ABPS exhibited better effects on accelerating glucose consumption and decreasing the triglyceride content than WBPS via stimulating glucose consumption (GLUT4) and gluconeogenesis (PEPCK).					
33172614	6	36	theme	glucose-treated	918:932	arg1	elegans					937:943	glucose-treated C. elegans	918:943	glucose-treated C. elegans	918:943	In the model of glucose-treated C. elegans, we observed that both WBPS and ABPS obviously suppressed the fat accumulation, more significantly by ABPS, along with no toxicity towards some physical activities.					
33172614	4	37	from	contents	541:548	arg1	different					512:520	different	512:520	different	512:520	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	0	38	theme	lipid	77:81	arg1	accumulation					83:94	lipid accumulation	77:94	lipid accumulation	77:94	Water-soluble and alkali-soluble polysaccharides from bitter melon inhibited lipid accumulation in HepG2 cells and Caenorhabditis elegans.					
33172614	7	39	theme	BPSs	1245:1248	arg1	effects					1234:1240	the lipid-lowering effects	1215:1240	the lipid-lowering effects of BPSs	1215:1248	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways were further confirmed to be involved in the lipid-lowering effects of BPSs.					
33172614	3	40	theme	fat-lowering	414:425	arg1	bioactivities					427:439	the fat-lowering bioactivities	410:439	the fat-lowering bioactivities in HepG2 cells and Caenorhabditis elegans	410:481	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides (ABPS) were extracted to evaluate the fat-lowering bioactivities in HepG2 cells and Caenorhabditis elegans.					
33172614	4	41	dep	Mw	616:617	arg1	1552 kDa					632:639	1552 kDa	632:639	1552 kDa	632:639	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	41	dep	Mw	616:617	arg1	332 kDa					620:626	332 kDa	620:626	332 kDa	620:626	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	1	42	theme	Bitter	139:144	arg1	BPS					169:171	BPS	169:171	BPS	169:171	Bitter melon polysaccharides (BPS) have been reported to have hypolipidemic effects.					
33172614	1	42	theme	Bitter	139:144	arg1	polysaccharides					152:166	Bitter melon polysaccharides	139:166	Bitter melon polysaccharides (BPS)	139:172	Bitter melon polysaccharides (BPS) have been reported to have hypolipidemic effects.					
33172614	7	43	theme	Fat-5	1110:1114	arg1	pathways					1165:1172	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways	1110:1172	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways	1110:1172	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways were further confirmed to be involved in the lipid-lowering effects of BPSs.					
33172614	2	44	theme	lipid	273:277	arg1	metabolism					279:288	lipid metabolism	273:288	lipid metabolism	273:288	However, the precise mechanism of BPS regulating lipid metabolism remains elusive.					
33172614	1	45	theme	melon	146:150	arg1	BPS					169:171	BPS	169:171	BPS	169:171	Bitter melon polysaccharides (BPS) have been reported to have hypolipidemic effects.					
33172614	1	45	theme	melon	146:150	arg1	polysaccharides					152:166	Bitter melon polysaccharides	139:166	Bitter melon polysaccharides (BPS)	139:172	Bitter melon polysaccharides (BPS) have been reported to have hypolipidemic effects.					
33172614	8	46	from	effects	1312:1318	arg1	lowering					1328:1335	fat- lowering	1323:1335	fat- lowering in HepG2 cells	1323:1350	Our studies demonstrated that both WBPS and ABPS can exhibit effects on fat- lowering in HepG2 cells and C. elegans.					
33172614	8	46	from	effects	1312:1318	arg1	elegans					1359:1365	C. elegans	1356:1365	C. elegans	1356:1365	Our studies demonstrated that both WBPS and ABPS can exhibit effects on fat- lowering in HepG2 cells and C. elegans.					
33172614	4	47	dep	weight	608:613	arg1	Mw					616:617	Mw	616:617	Mw	616:617	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	7	48	theme	lipid-lowering	1219:1232	arg1	effects					1234:1240	the lipid-lowering effects	1215:1240	the lipid-lowering effects of BPSs	1215:1248	Fat-5, fat-6 and fat-7 mediated fatty acid desaturases pathways were further confirmed to be involved in the lipid-lowering effects of BPSs.					
33172614	4	49	theme	molecular	598:606	arg1	weight					608:613	molecular weight	598:613	molecular weight (Mw: 332 kDa and 1552 kDa, respectively)	598:654	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	49	theme	molecular	598:606	arg1	contents					541:548	the uronic acid contents	525:548	the uronic acid contents (22.23% and 5.69%)	525:567	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	3	50	theme	bitter	347:352	arg1	polysaccharides					360:374	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides	307:374	polysaccharides	360:374	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides (ABPS) were extracted to evaluate the fat-lowering bioactivities in HepG2 cells and Caenorhabditis elegans.					
33172614	6	51	theme	fat	1007:1009	arg1	accumulation					1011:1022	the fat accumulation	1003:1022	the fat accumulation	1003:1022	In the model of glucose-treated C. elegans, we observed that both WBPS and ABPS obviously suppressed the fat accumulation, more significantly by ABPS, along with no toxicity towards some physical activities.					
33172614	2	52	theme	BPS	258:260	arg1	mechanism					245:253	the precise mechanism	233:253	the precise mechanism of BPS regulating lipid metabolism	233:288	However, the precise mechanism of BPS regulating lipid metabolism remains elusive.					
33172614	3	53	theme	melon	354:358	arg1	polysaccharides					360:374	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides	307:374	polysaccharides	360:374	Water-soluble (WBPS) and alkali-soluble bitter melon polysaccharides (ABPS) were extracted to evaluate the fat-lowering bioactivities in HepG2 cells and Caenorhabditis elegans.					
33172614	4	54	theme	IR	660:661	arg1	contents					541:548	the uronic acid contents	525:548	the uronic acid contents (22.23% and 5.69%)	525:567	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
33172614	4	54	theme	IR	660:661	arg1	spectra					663:669	IR spectra	660:669	IR spectra	660:669	WBPS and ABPS were slightly different in the uronic acid contents (22.23% and 5.69%), monosaccharide composition, molecular weight (Mw: 332 kDa and 1552 kDa, respectively) and IR spectra.					
32337764	0	0	from	role	18:21	arg1	tolerance					71:79	desiccation tolerance	59:79	desiccation tolerance in lichen-forming microalgae	59:108	Disentangling the role of extracellular polysaccharides in desiccation tolerance in lichen-forming microalgae.					
32337764	3	1	theme	flexible	271:278	arg1	walls					285:289	flexible cell walls	271:289	flexible cell walls	271:289	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	5	2	theme	size	640:643	arg1	stains					699:704	specific stains	690:704	specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition)	690:849	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	5	2	theme	size	640:643	arg1	exclusion					645:653	size exclusion and anion exchange chromatography	640:687	exclusion	645:653	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	11	3	theme	desiccation-tolerant	1740:1759	arg1	microalgae					1761:1770	aeroterrestrial desiccation-tolerant microalgae	1724:1770	aeroterrestrial desiccation-tolerant microalgae	1724:1770	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae is species-specific and seems to play a role in the response to changes in environmental water availability.					
32337764	5	4	theme	trimethylsilyl	776:789	arg1	derivatives					791:801	trimethylsilyl derivatives	776:801	trimethylsilyl derivatives	776:801	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	0	5	theme	lichen-forming	84:97	arg1	microalgae					99:108	lichen-forming microalgae	84:108	lichen-forming microalgae	84:108	Disentangling the role of extracellular polysaccharides in desiccation tolerance in lichen-forming microalgae.					
32337764	11	6	theme	water	1861:1865	arg1	availability					1867:1878	environmental water availability	1847:1878	environmental water availability	1847:1878	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae is species-specific and seems to play a role in the response to changes in environmental water availability.					
32337764	3	7	theme	cell	280:283	arg1	walls					285:289	flexible cell walls	271:289	flexible cell walls	271:289	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	10	8	theme	first	1440:1444	arg1	time					1446:1449	the first time	1436:1449	the first time	1436:1449	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	4	9	mod	modified	576:583	arg1	polysaccharides					488:502	extracellular polysaccharides	474:502	extracellular polysaccharides excreted by each microalga	474:529	Here, we tested the hypothesis that extracellular polysaccharides excreted by each microalga could be quantitatively and/or qualitatively modified by D/R.					
32337764	4	9	mod	modified	576:583	arg3	D/R					588:590	D/R	588:590	D/R	588:590	Here, we tested the hypothesis that extracellular polysaccharides excreted by each microalga could be quantitatively and/or qualitatively modified by D/R.					
32337764	8	10	theme	polysaccharides	1146:1160	arg1	profile					1121:1127	the polydispersed profile	1103:1127	the polydispersed profile of extracellular polysaccharides in either microalga	1103:1180	D/R did not alter the polydispersed profile of extracellular polysaccharides in either microalga but did induce quantitative changes in several peaks.					
32337764	8	11	theme	quantitative	1197:1208	arg1	changes					1210:1216	quantitative changes	1197:1216	quantitative changes in several peaks	1197:1233	D/R did not alter the polydispersed profile of extracellular polysaccharides in either microalga but did induce quantitative changes in several peaks.					
32337764	5	12	theme	gel	712:714	arg1	electrophoresis					716:730	gel electrophoresis	712:730	gel electrophoresis	712:730	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	9	13	theme	higher	1343:1348	arg1	mass					1360:1363	higher molecular mass	1343:1363	higher molecular mass carbohydrates after D/R	1343:1387	Furthermore, medium-low-sized uronic acid-containing polysaccharides were almost completely substituted by higher molecular mass carbohydrates after D/R.					
32337764	2	14	theme	Trebouxia	190:198	arg1	sp					200:201	Trebouxia sp	190:201	Trebouxia sp.	190:202	Trebouxia sp.					
32337764	9	15	theme	acid-containing	1273:1287	arg1	polysaccharides					1289:1303	medium-low-sized uronic acid-containing polysaccharides	1249:1303	medium-low-sized uronic acid-containing polysaccharides	1249:1303	Furthermore, medium-low-sized uronic acid-containing polysaccharides were almost completely substituted by higher molecular mass carbohydrates after D/R.					
32337764	4	16	theme	extracellular	474:486	arg1	polysaccharides					488:502	extracellular polysaccharides	474:502	extracellular polysaccharides excreted by each microalga	474:529	Here, we tested the hypothesis that extracellular polysaccharides excreted by each microalga could be quantitatively and/or qualitatively modified by D/R.					
32337764	11	17	theme	environmental	1847:1859	arg1	availability					1867:1878	environmental water availability	1847:1878	environmental water availability	1847:1878	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae is species-specific and seems to play a role in the response to changes in environmental water availability.					
32337764	10	18	theme	polymeric	1473:1481	arg1	substances					1483:1492	the extracellular polymeric substances	1455:1492	the extracellular polymeric substances of both microalgae	1455:1511	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	10	19	theme	polysaccharide	1399:1412	arg1	s					1414:1414	Sulfated polysaccharide(s)	1390:1415	Sulfated polysaccharide(s)	1390:1415	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	10	20	theme	sugar-sulfotransferase	1588:1609	arg1	gene					1611:1614	a sugar-sulfotransferase gene	1586:1614	a sugar-sulfotransferase gene	1586:1614	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	5	21	theme	gas	736:738	arg1	spectrometry					760:771	gas chromatography/mass spectrometry	736:771	gas chromatography/mass spectrometry	736:771	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	10	22	theme	cyclic	1560:1565	arg1	D/R					1567:1569	cyclic D/R	1560:1569	cyclic D/R	1560:1569	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	10	23	theme	sulfated	1632:1639	arg1	s					1660:1660	sulfated ß-D-galactofuranan(s)	1632:1661	sulfated ß-D-galactofuranan(s)	1632:1661	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	10	24	theme	extracellular	1459:1471	arg1	substances					1483:1492	the extracellular polymeric substances	1455:1492	the extracellular polymeric substances of both microalgae	1455:1511	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	3	25	theme	biochemical	413:423	arg1	composition					425:435	biochemical composition	413:435	biochemical composition	413:435	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	5	26	theme	chromatography/mass	740:758	arg1	spectrometry					760:771	gas chromatography/mass spectrometry	736:771	gas chromatography/mass spectrometry	736:771	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	5	27	theme	Extracellular	593:605	arg1	polysaccharides					607:621	Extracellular polysaccharides	593:621	Extracellular polysaccharides	593:621	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	11	28	from	changes	1836:1842	arg1	availability					1867:1878	environmental water availability	1847:1878	environmental water availability	1847:1878	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae is species-specific and seems to play a role in the response to changes in environmental water availability.					
32337764	3	29	dep	microalgae	255:264	arg1	undergo					298:304	undergo	298:304	undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition	298:435	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	10	30	theme	ß-D-galactofuranan	1641:1658	arg1	s					1660:1660	sulfated ß-D-galactofuranan(s)	1632:1661	sulfated ß-D-galactofuranan(s)	1632:1661	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	3	31	theme	desiccation-tolerant	234:253	arg1	TR9					204:206	TR9	204:206	TR9	204:206	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	3	31	theme	desiccation-tolerant	234:253	arg1	simplex					222:228	Coccomyxa simplex	212:228	Coccomyxa simplex	212:228	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	3	31	theme	desiccation-tolerant	234:253	arg1	microalgae					255:264	desiccation-tolerant microalgae	234:264	desiccation-tolerant microalgae with flexible cell walls	234:289	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	9	32	theme	medium-low-sized	1249:1264	arg1	polysaccharides					1289:1303	medium-low-sized uronic acid-containing polysaccharides	1249:1303	medium-low-sized uronic acid-containing polysaccharides	1249:1303	Furthermore, medium-low-sized uronic acid-containing polysaccharides were almost completely substituted by higher molecular mass carbohydrates after D/R.					
32337764	8	33	theme	several	1221:1227	arg1	peaks					1229:1233	several peaks	1221:1233	several peaks	1221:1233	D/R did not alter the polydispersed profile of extracellular polysaccharides in either microalga but did induce quantitative changes in several peaks.					
32337764	3	34	theme	Coccomyxa	212:220	arg1	TR9					204:206	TR9	204:206	TR9	204:206	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	3	34	theme	Coccomyxa	212:220	arg1	simplex					222:228	Coccomyxa simplex	212:228	Coccomyxa simplex	212:228	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	3	34	theme	Coccomyxa	212:220	arg1	microalgae					255:264	desiccation-tolerant microalgae	234:264	desiccation-tolerant microalgae with flexible cell walls	234:289	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	1	35	theme	First	111:115	arg1	evidence					117:124	First evidence	111:124	First evidence of sulfated polysaccharides and ancient sulfotransferase genes	111:187	First evidence of sulfated polysaccharides and ancient sulfotransferase genes.					
32337764	7	36	theme	sugar-sulfotransferase	965:986	arg1	genes					997:1001	sugar-sulfotransferase encoding genes	965:1001	sugar-sulfotransferase encoding genes	965:1001	In addition, sugar-sulfotransferase encoding genes were identified in both microalgae, and their expression was measured by RT-qPCR.					
32337764	5	37	theme	derivatives	791:801	arg1	spectrometry					760:771	gas chromatography/mass spectrometry	736:771	gas chromatography/mass spectrometry	736:771	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	5	37	theme	derivatives	791:801	arg1	electrophoresis					716:730	gel electrophoresis	712:730	gel electrophoresis	712:730	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	5	38	theme	specific	690:697	arg1	stains					699:704	specific stains	690:704	specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition)	690:849	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	5	38	theme	specific	690:697	arg1	exclusion					645:653	size exclusion and anion exchange chromatography	640:687	exclusion	645:653	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	6	39	theme	magnetic	917:924	arg1	NMR					937:939	NMR	937:939	NMR	937:939	The structure of a TR9-sulfated polymer was deduced from nuclear magnetic resonance (NMR) analyses.					
32337764	6	39	theme	magnetic	917:924	arg1	resonance					926:934	nuclear magnetic resonance	909:934	nuclear magnetic resonance (NMR) analyses	909:949	The structure of a TR9-sulfated polymer was deduced from nuclear magnetic resonance (NMR) analyses.					
32337764	7	40	theme	encoding	988:995	arg1	genes					997:1001	sugar-sulfotransferase encoding genes	965:1001	sugar-sulfotransferase encoding genes	965:1001	In addition, sugar-sulfotransferase encoding genes were identified in both microalgae, and their expression was measured by RT-qPCR.					
32337764	6	41	theme	resonance	926:934	arg1	analyses					942:949	nuclear magnetic resonance (NMR) analyses	909:949	nuclear magnetic resonance (NMR) analyses	909:949	The structure of a TR9-sulfated polymer was deduced from nuclear magnetic resonance (NMR) analyses.					
32337764	3	42	with	microalgae	255:264	arg1	walls					285:289	flexible cell walls	271:289	flexible cell walls	271:289	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	0	43	theme	polysaccharides	40:54	arg1	role					18:21	the role	14:21	the role of extracellular polysaccharides in desiccation tolerance in lichen-forming microalgae	14:108	Disentangling the role of extracellular polysaccharides in desiccation tolerance in lichen-forming microalgae.					
32337764	3	44	theme	species-specific	306:321	arg1	remodelling					323:333	species-specific remodelling	306:333	species-specific remodelling	306:333	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	11	45	theme	polysaccharides	1705:1719	arg1	remodelling					1676:1686	Biochemical remodelling	1664:1686	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae	1664:1770	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae is species-specific and seems to play a role in the response to changes in environmental water availability.					
32337764	0	46	theme	extracellular	26:38	arg1	polysaccharides					40:54	extracellular polysaccharides	26:54	extracellular polysaccharides	26:54	Disentangling the role of extracellular polysaccharides in desiccation tolerance in lichen-forming microalgae.					
32337764	8	47	theme	extracellular	1132:1144	arg1	polysaccharides					1146:1160	extracellular polysaccharides	1132:1160	extracellular polysaccharides	1132:1160	D/R did not alter the polydispersed profile of extracellular polysaccharides in either microalga but did induce quantitative changes in several peaks.					
32337764	9	48	theme	uronic	1266:1271	arg1	polysaccharides					1289:1303	medium-low-sized uronic acid-containing polysaccharides	1249:1303	medium-low-sized uronic acid-containing polysaccharides	1249:1303	Furthermore, medium-low-sized uronic acid-containing polysaccharides were almost completely substituted by higher molecular mass carbohydrates after D/R.					
32337764	6	49	theme	nuclear	909:915	arg1	NMR					937:939	NMR	937:939	NMR	937:939	The structure of a TR9-sulfated polymer was deduced from nuclear magnetic resonance (NMR) analyses.					
32337764	6	49	theme	nuclear	909:915	arg1	resonance					926:934	nuclear magnetic resonance	909:934	nuclear magnetic resonance (NMR) analyses	909:949	The structure of a TR9-sulfated polymer was deduced from nuclear magnetic resonance (NMR) analyses.					
32337764	9	50	theme	molecular	1350:1358	arg1	mass					1360:1363	higher molecular mass	1343:1363	higher molecular mass carbohydrates after D/R	1343:1387	Furthermore, medium-low-sized uronic acid-containing polysaccharides were almost completely substituted by higher molecular mass carbohydrates after D/R.					
32337764	0	51	theme	desiccation	59:69	arg1	tolerance					71:79	desiccation tolerance	59:79	desiccation tolerance in lichen-forming microalgae	59:108	Disentangling the role of extracellular polysaccharides in desiccation tolerance in lichen-forming microalgae.					
32337764	8	52	theme	polydispersed	1107:1119	arg1	profile					1121:1127	the polydispersed profile	1103:1127	the polydispersed profile of extracellular polysaccharides in either microalga	1103:1180	D/R did not alter the polydispersed profile of extracellular polysaccharides in either microalga but did induce quantitative changes in several peaks.					
32337764	0	53	from	tolerance	71:79	arg1	microalgae					99:108	lichen-forming microalgae	84:108	lichen-forming microalgae	84:108	Disentangling the role of extracellular polysaccharides in desiccation tolerance in lichen-forming microalgae.					
32337764	11	54	from	remodelling	1676:1686	arg1	microalgae					1761:1770	aeroterrestrial desiccation-tolerant microalgae	1724:1770	aeroterrestrial desiccation-tolerant microalgae	1724:1770	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae is species-specific and seems to play a role in the response to changes in environmental water availability.					
32337764	1	55	theme	sulfated	129:136	arg1	polysaccharides					138:152	sulfated polysaccharides	129:152	sulfated polysaccharides	129:152	First evidence of sulfated polysaccharides and ancient sulfotransferase genes.					
32337764	5	56	theme	anion	659:663	arg1	chromatography					674:687	size exclusion and anion exchange chromatography	640:687	chromatography	674:687	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	1	57	theme	polysaccharides	138:152	arg1	evidence					117:124	First evidence	111:124	First evidence of sulfated polysaccharides and ancient sulfotransferase genes	111:187	First evidence of sulfated polysaccharides and ancient sulfotransferase genes.					
32337764	5	58	dep	electrophoresis	716:730	arg1	determine					807:815	determine	807:815	to determine their monosaccharide composition	804:848	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	8	59	from	changes	1210:1216	arg1	peaks					1229:1233	several peaks	1221:1233	several peaks	1221:1233	D/R did not alter the polydispersed profile of extracellular polysaccharides in either microalga but did induce quantitative changes in several peaks.					
32337764	5	60	theme	monosaccharide	823:836	arg1	composition					838:848	their monosaccharide composition	817:848	their monosaccharide composition	817:848	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	5	61	theme	exchange	665:672	arg1	chromatography					674:687	size exclusion and anion exchange chromatography	640:687	chromatography	674:687	Extracellular polysaccharides were analysed by size exclusion and anion exchange chromatography, specific stains after gel electrophoresis and gas chromatography/mass spectrometry of trimethylsilyl derivatives (to determine their monosaccharide composition).					
32337764	8	62	from	profile	1121:1127	arg1	microalga					1172:1180	either microalga	1165:1180	either microalga	1165:1180	D/R did not alter the polydispersed profile of extracellular polysaccharides in either microalga but did induce quantitative changes in several peaks.					
32337764	11	63	theme	extracellular	1691:1703	arg1	polysaccharides					1705:1719	extracellular polysaccharides	1691:1719	extracellular polysaccharides	1691:1719	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae is species-specific and seems to play a role in the response to changes in environmental water availability.					
32337764	10	64	theme	Sulfated	1390:1397	arg1	s					1414:1414	Sulfated polysaccharide(s)	1390:1415	Sulfated polysaccharide(s)	1390:1415	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	6	65	theme	polymer	884:890	arg1	structure					856:864	The structure	852:864	The structure of a TR9-sulfated polymer	852:890	The structure of a TR9-sulfated polymer was deduced from nuclear magnetic resonance (NMR) analyses.					
32337764	1	66	theme	ancient	158:164	arg1	genes					183:187	ancient sulfotransferase genes	158:187	ancient sulfotransferase genes	158:187	First evidence of sulfated polysaccharides and ancient sulfotransferase genes.					
32337764	11	67	theme	Biochemical	1664:1674	arg1	remodelling					1676:1686	Biochemical remodelling	1664:1686	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae	1664:1770	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae is species-specific and seems to play a role in the response to changes in environmental water availability.					
32337764	10	68	theme	microalgae	1502:1511	arg1	substances					1483:1492	the extracellular polymeric substances	1455:1492	the extracellular polymeric substances of both microalgae	1455:1511	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	3	69	theme	distinct	385:392	arg1	ultrastructure					394:407	their distinct ultrastructure	379:407	their distinct ultrastructure	379:407	TR9 and Coccomyxa simplex are desiccation-tolerant microalgae with flexible cell walls, which undergo species-specific remodelling during dehydration-rehydration (D/R) due to their distinct ultrastructure and biochemical composition.					
32337764	10	70	located	detected	1422:1429	arg2	s					1414:1414	Sulfated polysaccharide(s)	1390:1415	Sulfated polysaccharide(s)	1390:1415	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	10	70	located	detected	1422:1429	arg1	increased					1523:1531	increased	1523:1531	increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s)	1523:1661	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	10	70	located	detected	1422:1429	arg1	substances					1483:1492	the extracellular polymeric substances	1455:1492	the extracellular polymeric substances of both microalgae	1455:1511	Sulfated polysaccharide(s) were detected, for the first time, in the extracellular polymeric substances of both microalgae, but only increased significantly in TR9 after cyclic D/R, which induced a sugar-sulfotransferase gene and accumulated sulfated ß-D-galactofuranan(s).					
32337764	6	71	theme	TR9-sulfated	871:882	arg1	polymer					884:890	a TR9-sulfated polymer	869:890	a TR9-sulfated polymer	869:890	The structure of a TR9-sulfated polymer was deduced from nuclear magnetic resonance (NMR) analyses.					
32337764	1	72	theme	sulfotransferase	166:181	arg1	genes					183:187	ancient sulfotransferase genes	158:187	ancient sulfotransferase genes	158:187	First evidence of sulfated polysaccharides and ancient sulfotransferase genes.					
32337764	1	73	theme	genes	183:187	arg1	evidence					117:124	First evidence	111:124	First evidence of sulfated polysaccharides and ancient sulfotransferase genes	111:187	First evidence of sulfated polysaccharides and ancient sulfotransferase genes.					
32337764	11	74	theme	aeroterrestrial	1724:1738	arg1	microalgae					1761:1770	aeroterrestrial desiccation-tolerant microalgae	1724:1770	aeroterrestrial desiccation-tolerant microalgae	1724:1770	Biochemical remodelling of extracellular polysaccharides in aeroterrestrial desiccation-tolerant microalgae is species-specific and seems to play a role in the response to changes in environmental water availability.					
32718654	0	0	theme	antibacterial	104:116	arg1	therapeutics					118:129	synergistic periodontal antibacterial therapeutics	80:129	synergistic periodontal antibacterial therapeutics	80:129	Construction of functional curdlan hydrogels with bio-inspired polydopamine for synergistic periodontal antibacterial therapeutics.					
32718654	1	1	theme	substantial	195:205	arg1	potential					207:215	substantial potential	195:215	substantial potential for periodontal antimicrobial delivery	195:254	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	7	2	theme	bacteriostatic	1003:1016	arg1	rate					1018:1021	The optimized bacteriostatic rate	989:1021	The optimized bacteriostatic rate	989:1021	The optimized bacteriostatic rate reached 99.9 %.					
32718654	0	3	theme	periodontal	92:102	arg1	therapeutics					118:129	synergistic periodontal antibacterial therapeutics	80:129	synergistic periodontal antibacterial therapeutics	80:129	Construction of functional curdlan hydrogels with bio-inspired polydopamine for synergistic periodontal antibacterial therapeutics.					
32718654	8	4	theme	photothermal	1149:1160	arg1	effect					1162:1167	photothermal effect	1149:1167	photothermal effect	1149:1167	Overall, we aimed to provide new curdlan-based hydrogels for periodontal antibacterial treatment by combining photothermal effect and antimicrobial simultaneously.					
32718654	3	5	theme	tunable	434:440	arg1	properties					442:451	their tunable properties	428:451	their tunable properties associated with concentration of PDA including pore size, rheological property and swelling behavior	428:552	The physiochemical evaluations of composite hydrogels proved their tunable properties associated with concentration of PDA including pore size, rheological property and swelling behavior.					
32718654	5	6	theme	near	694:697	arg1	exposure					720:727	near infrared light (NIR) exposure	694:727	near infrared light (NIR) exposure	694:727	Moreover, photothermal performance upon near infrared light (NIR) exposure was conducted and eventually indicated the best matches for antibacterial application.					
32718654	5	7	theme	best	772:775	arg1	matches					777:783	the best matches	768:783	the best matches for antibacterial application	768:813	Moreover, photothermal performance upon near infrared light (NIR) exposure was conducted and eventually indicated the best matches for antibacterial application.					
32718654	5	8	theme	NIR	715:717	arg1	exposure					720:727	near infrared light (NIR) exposure	694:727	near infrared light (NIR) exposure	694:727	Moreover, photothermal performance upon near infrared light (NIR) exposure was conducted and eventually indicated the best matches for antibacterial application.					
32718654	8	9	theme	periodontal	1100:1110	arg1	treatment					1126:1134	periodontal antibacterial treatment	1100:1134	periodontal antibacterial treatment	1100:1134	Overall, we aimed to provide new curdlan-based hydrogels for periodontal antibacterial treatment by combining photothermal effect and antimicrobial simultaneously.					
32718654	2	10	theme	hydrogels	343:351	arg1	engineering					274:284	the facile engineering	263:284	the facile engineering of a functionalized curdlan/polydopamine (PDA) composite hydrogels	263:351	Here, the facile engineering of a functionalized curdlan/polydopamine (PDA) composite hydrogels was reported.					
32718654	8	11	theme	antibacterial	1112:1124	arg1	treatment					1126:1134	periodontal antibacterial treatment	1100:1134	periodontal antibacterial treatment	1100:1134	Overall, we aimed to provide new curdlan-based hydrogels for periodontal antibacterial treatment by combining photothermal effect and antimicrobial simultaneously.					
32718654	2	12	theme	composite	333:341	arg1	hydrogels					343:351	a functionalized curdlan/polydopamine (PDA) composite hydrogels	289:351	a functionalized curdlan/polydopamine (PDA) composite hydrogels	289:351	Here, the facile engineering of a functionalized curdlan/polydopamine (PDA) composite hydrogels was reported.					
32718654	1	13	contain	possesses	185:193	arg2	potential					207:215	substantial potential	195:215	substantial potential for periodontal antimicrobial delivery	195:254	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	1	13	contain	possesses	185:193	arg1	resource					175:182	a bacteria-derived polysaccharide resource	141:182	a bacteria-derived polysaccharide resource	141:182	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	1	13	contain	possesses	185:193	arg1	Curdlan					132:138	Curdlan	132:138	Curdlan	132:138	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	6	14	theme	release	892:898	arg1	profiles					900:907	the release profiles	888:907	the release profiles	888:907	The acetate chlorhexidine (CHX) was chosen as a model antimicrobial and the release profiles demonstrated the entrapped CHX could be triggered and nicely controlled by NIR.					
32718654	6	15	theme	acetate	820:826	arg1	CHX					843:845	CHX	843:845	CHX	843:845	The acetate chlorhexidine (CHX) was chosen as a model antimicrobial and the release profiles demonstrated the entrapped CHX could be triggered and nicely controlled by NIR.					
32718654	6	15	theme	acetate	820:826	arg1	chlorhexidine					828:840	The acetate chlorhexidine	816:840	The acetate chlorhexidine (CHX)	816:846	The acetate chlorhexidine (CHX) was chosen as a model antimicrobial and the release profiles demonstrated the entrapped CHX could be triggered and nicely controlled by NIR.					
32718654	6	15	theme	acetate	820:826	arg1	antimicrobial					870:882	a model antimicrobial	862:882	a model antimicrobial	862:882	The acetate chlorhexidine (CHX) was chosen as a model antimicrobial and the release profiles demonstrated the entrapped CHX could be triggered and nicely controlled by NIR.					
32718654	1	16	theme	periodontal	221:231	arg1	delivery					247:254	periodontal antimicrobial delivery	221:254	periodontal antimicrobial delivery	221:254	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	3	17	theme	composite	401:409	arg1	hydrogels					411:419	composite hydrogels	401:419	composite hydrogels	401:419	The physiochemical evaluations of composite hydrogels proved their tunable properties associated with concentration of PDA including pore size, rheological property and swelling behavior.					
32718654	5	18	theme	antibacterial	789:801	arg1	application					803:813	antibacterial application	789:813	antibacterial application	789:813	Moreover, photothermal performance upon near infrared light (NIR) exposure was conducted and eventually indicated the best matches for antibacterial application.					
32718654	1	19	theme	antimicrobial	233:245	arg1	delivery					247:254	periodontal antimicrobial delivery	221:254	periodontal antimicrobial delivery	221:254	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	5	20	theme	infrared	699:706	arg1	exposure					720:727	near infrared light (NIR) exposure	694:727	near infrared light (NIR) exposure	694:727	Moreover, photothermal performance upon near infrared light (NIR) exposure was conducted and eventually indicated the best matches for antibacterial application.					
32718654	3	21	theme	rheological	511:521	arg1	property					523:530	rheological property	511:530	rheological property	511:530	The physiochemical evaluations of composite hydrogels proved their tunable properties associated with concentration of PDA including pore size, rheological property and swelling behavior.					
32718654	0	22	theme	curdlan	27:33	arg1	Construction					0:11	Construction	0:11	Construction of functional curdlan	0:33	Construction of functional curdlan hydrogels with bio-inspired polydopamine for synergistic periodontal antibacterial therapeutics.					
32718654	5	23	theme	light	708:712	arg1	exposure					720:727	near infrared light (NIR) exposure	694:727	near infrared light (NIR) exposure	694:727	Moreover, photothermal performance upon near infrared light (NIR) exposure was conducted and eventually indicated the best matches for antibacterial application.					
32718654	0	24	theme	functional	16:25	arg1	curdlan					27:33	functional curdlan	16:33	functional curdlan	16:33	Construction of functional curdlan hydrogels with bio-inspired polydopamine for synergistic periodontal antibacterial therapeutics.					
32718654	3	25	theme	pore	500:503	arg1	size					505:508	pore size	500:508	pore size	500:508	The physiochemical evaluations of composite hydrogels proved their tunable properties associated with concentration of PDA including pore size, rheological property and swelling behavior.					
32718654	3	26	theme	swelling	536:543	arg1	behavior					545:552	swelling behavior	536:552	swelling behavior	536:552	The physiochemical evaluations of composite hydrogels proved their tunable properties associated with concentration of PDA including pore size, rheological property and swelling behavior.					
32718654	3	27	theme	PDA	486:488	arg1	concentration					469:481	concentration	469:481	concentration of PDA including pore size, rheological property and swelling behavior	469:552	The physiochemical evaluations of composite hydrogels proved their tunable properties associated with concentration of PDA including pore size, rheological property and swelling behavior.					
32718654	8	28	theme	curdlan-based	1072:1084	arg1	hydrogels					1086:1094	new curdlan-based hydrogels	1068:1094	new curdlan-based hydrogels	1068:1094	Overall, we aimed to provide new curdlan-based hydrogels for periodontal antibacterial treatment by combining photothermal effect and antimicrobial simultaneously.					
32718654	6	29	theme	model	864:868	arg1	chlorhexidine					828:840	The acetate chlorhexidine	816:840	The acetate chlorhexidine (CHX)	816:846	The acetate chlorhexidine (CHX) was chosen as a model antimicrobial and the release profiles demonstrated the entrapped CHX could be triggered and nicely controlled by NIR.					
32718654	6	29	theme	model	864:868	arg1	antimicrobial					870:882	a model antimicrobial	862:882	a model antimicrobial	862:882	The acetate chlorhexidine (CHX) was chosen as a model antimicrobial and the release profiles demonstrated the entrapped CHX could be triggered and nicely controlled by NIR.					
32718654	0	30	theme	bio-inspired	50:61	arg1	polydopamine					63:74	bio-inspired polydopamine	50:74	bio-inspired polydopamine for synergistic periodontal antibacterial therapeutics	50:129	Construction of functional curdlan hydrogels with bio-inspired polydopamine for synergistic periodontal antibacterial therapeutics.					
32718654	3	31	theme	physiochemical	371:384	arg1	evaluations					386:396	The physiochemical evaluations	367:396	The physiochemical evaluations of composite hydrogels	367:419	The physiochemical evaluations of composite hydrogels proved their tunable properties associated with concentration of PDA including pore size, rheological property and swelling behavior.					
32718654	2	32	theme	facile	267:272	arg1	engineering					274:284	the facile engineering	263:284	the facile engineering of a functionalized curdlan/polydopamine (PDA) composite hydrogels	263:351	Here, the facile engineering of a functionalized curdlan/polydopamine (PDA) composite hydrogels was reported.					
32718654	5	33	theme	photothermal	664:675	arg1	performance					677:687	photothermal performance	664:687	photothermal performance upon near infrared light (NIR) exposure	664:727	Moreover, photothermal performance upon near infrared light (NIR) exposure was conducted and eventually indicated the best matches for antibacterial application.					
32718654	1	34	theme	bacteria-derived	143:158	arg1	resource					175:182	a bacteria-derived polysaccharide resource	141:182	a bacteria-derived polysaccharide resource	141:182	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	1	34	theme	bacteria-derived	143:158	arg1	Curdlan					132:138	Curdlan	132:138	Curdlan	132:138	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	6	35	theme	entrapped	926:934	arg1	CHX					936:938	the entrapped CHX	922:938	the entrapped CHX	922:938	The acetate chlorhexidine (CHX) was chosen as a model antimicrobial and the release profiles demonstrated the entrapped CHX could be triggered and nicely controlled by NIR.					
32718654	1	36	theme	polysaccharide	160:173	arg1	resource					175:182	a bacteria-derived polysaccharide resource	141:182	a bacteria-derived polysaccharide resource	141:182	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	1	36	theme	polysaccharide	160:173	arg1	Curdlan					132:138	Curdlan	132:138	Curdlan	132:138	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	1	37	link	bacteria-derived	143:158	arg1	resource					175:182	a bacteria-derived polysaccharide resource	141:182	a bacteria-derived polysaccharide resource	141:182	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	1	37	link	bacteria-derived	143:158	arg1	Curdlan					132:138	Curdlan	132:138	Curdlan	132:138	Curdlan, a bacteria-derived polysaccharide resource, possesses substantial potential for periodontal antimicrobial delivery.					
32718654	0	38	theme	synergistic	80:90	arg1	therapeutics					118:129	synergistic periodontal antibacterial therapeutics	80:129	synergistic periodontal antibacterial therapeutics	80:129	Construction of functional curdlan hydrogels with bio-inspired polydopamine for synergistic periodontal antibacterial therapeutics.					
32718654	2	39	theme	curdlan/polydopamine	306:325	arg1	hydrogels					343:351	a functionalized curdlan/polydopamine (PDA) composite hydrogels	289:351	a functionalized curdlan/polydopamine (PDA) composite hydrogels	289:351	Here, the facile engineering of a functionalized curdlan/polydopamine (PDA) composite hydrogels was reported.					
32718654	3	40	theme	hydrogels	411:419	arg1	evaluations					386:396	The physiochemical evaluations	367:396	The physiochemical evaluations of composite hydrogels	367:419	The physiochemical evaluations of composite hydrogels proved their tunable properties associated with concentration of PDA including pore size, rheological property and swelling behavior.					
32718654	2	41	theme	functionalized	291:304	arg1	curdlan/polydopamine					306:325	a functionalized curdlan/polydopamine	289:325	a functionalized curdlan/polydopamine (PDA) composite hydrogels	289:351	Here, the facile engineering of a functionalized curdlan/polydopamine (PDA) composite hydrogels was reported.					
32718654	2	41	theme	functionalized	291:304	arg1	PDA					328:330	PDA	328:330	PDA	328:330	Here, the facile engineering of a functionalized curdlan/polydopamine (PDA) composite hydrogels was reported.					
32718654	8	42	theme	new	1068:1070	arg1	hydrogels					1086:1094	new curdlan-based hydrogels	1068:1094	new curdlan-based hydrogels	1068:1094	Overall, we aimed to provide new curdlan-based hydrogels for periodontal antibacterial treatment by combining photothermal effect and antimicrobial simultaneously.					
32718654	7	43	theme	optimized	993:1001	arg1	rate					1018:1021	The optimized bacteriostatic rate	989:1021	The optimized bacteriostatic rate	989:1021	The optimized bacteriostatic rate reached 99.9 %.					
34768816	1	0	theme	moist	369:373	arg1	micro-environment					375:391	a moist micro-environment	367:391	a moist micro-environment	367:391	Marine polysaccharides are believed to be promising wound-dressing nanomaterials because of their biocompatibility, antibacterial and hemostatic activity, and ability to easily shape into transparent films, hydrogels, and porous foams that can provide a moist micro-environment and adsorb exudates.					
34768816	5	1	theme	clotting	1114:1121	arg1	times					1123:1127	clotting times	1114:1127	clotting times	1114:1127	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	7	2	theme	human	1367:1371	arg1	fibroblasts					1376:1386	human BJ fibroblasts	1367:1386	human BJ fibroblasts	1367:1386	Moreover, the cytotoxicity and genotoxicity of the materials against two cell lines, human BJ fibroblasts and human KERTr keratinocytes, was investigated.					
34768816	3	3	theme	wound	804:808	arg1	healing					810:816	wound healing	804:816	wound healing	804:816	In this contribution, we investigated the characteristics of six different cellulose-filled chitosan transparent films as potential factors that could help to accelerate wound healing.					
34768816	5	4	theme	system	1209:1214	arg1	pathways					1175:1182	both the extrinsic and intrinsic pathways	1142:1182	pathways	1175:1182	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	5	5	theme	blood	1191:1195	arg1	system					1209:1214	the blood coagulation system	1187:1214	the blood coagulation system	1187:1214	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	2	6	link	polysaccharide-derived	477:498	arg1	nanomaterials					500:512	novel polysaccharide-derived nanomaterials	471:512	novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems	471:631	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	3	7	theme	transparent	735:745	arg1	films					747:751	six different cellulose-filled chitosan transparent films	695:751	six different cellulose-filled chitosan transparent films	695:751	In this contribution, we investigated the characteristics of six different cellulose-filled chitosan transparent films as potential factors that could help to accelerate wound healing.					
34768816	1	8	theme	transparent	303:313	arg1	films					315:319	transparent films	303:319	transparent films	303:319	Marine polysaccharides are believed to be promising wound-dressing nanomaterials because of their biocompatibility, antibacterial and hemostatic activity, and ability to easily shape into transparent films, hydrogels, and porous foams that can provide a moist micro-environment and adsorb exudates.					
34768816	2	9	theme	ideal	605:609	arg1	healing					617:623	ideal wound healing	605:623	ideal wound healing systems	605:631	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	7	10	theme	KERTr	1398:1402	arg1	keratinocytes					1404:1416	human KERTr keratinocytes	1392:1416	human KERTr keratinocytes	1392:1416	Moreover, the cytotoxicity and genotoxicity of the materials against two cell lines, human BJ fibroblasts and human KERTr keratinocytes, was investigated.					
34768816	5	11	from	assessment	1009:1018	arg1	properties					1054:1063	hemostatic properties	1043:1063	hemostatic properties	1043:1063	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	9	12	theme	compounds	1682:1690	arg1	compounds					1682:1690	the compounds	1678:1690	the compounds	1678:1690	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	12	theme	compounds	1682:1690	arg1	films					1668:1672	phosphorylated microcellulose-filled chitosan films	1622:1672	phosphorylated microcellulose-filled chitosan films	1622:1672	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	12	theme	compounds	1682:1690	arg1	one					1617:1619	one	1617:1619	one	1617:1619	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	4	13	theme	nano-sized	845:854	arg1	cellulose					856:864	microcrystalline and nano-sized cellulose	824:864	cellulose	856:864	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	13	theme	nano-sized	845:854	arg1	cellulose					904:912	native and phosphorylated cellulose	878:912	native and phosphorylated cellulose	878:912	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	13	theme	nano-sized	845:854	arg1	fillers					928:934	fillers	928:934	fillers	928:934	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	14	theme	size	977:980	arg1	roles					968:972	the roles	964:972	the roles of size and functionalization	964:1002	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	5	15	theme	tested	1084:1089	arg1	nanocomposites					1091:1104	the tested nanocomposites	1080:1104	the tested nanocomposites	1080:1104	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	5	16	dep	pathways	1175:1182	arg1	both					1142:1145	both	1142:1145	both	1142:1145	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	11	17	theme	fibroblasts	2060:2070	arg1	migration					2042:2050	migration	2042:2050	migration of both fibroblasts and keratinocytes	2042:2088	These biocompatible films showed no genotoxicity, and very importantly for wound healing, most of them significantly accelerated migration of both fibroblasts and keratinocytes.					
34768816	4	18	theme	native	878:883	arg1	cellulose					904:912	native and phosphorylated cellulose	878:912	native and phosphorylated cellulose	878:912	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	18	theme	native	878:883	arg1	cellulose					856:864	microcrystalline and nano-sized cellulose	824:864	cellulose	856:864	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	18	theme	native	878:883	arg1	fillers					928:934	fillers	928:934	fillers	928:934	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	5	19	theme	influences	1029:1038	arg1	assessment					1009:1018	The assessment	1005:1018	The assessment of their influences on hemostatic properties	1005:1063	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	8	20	theme	cellulose	1455:1463	arg1	nature					1441:1446	The nature	1437:1446	The nature of the cellulose used as a filler	1437:1480	The nature of the cellulose used as a filler was found to influence their cytotoxicity at a relatively low level.					
34768816	2	21	theme	biological	578:587	arg1	requirements					589:600	the mechanical and biological requirements	559:600	the mechanical and biological requirements of ideal wound healing systems	559:631	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	10	22	theme	mitochondrial	1862:1874	arg1	potential					1876:1884	The transmembrane mitochondrial potential	1844:1884	The transmembrane mitochondrial potential	1844:1884	The transmembrane mitochondrial potential was very slightly lowered.					
34768816	5	23	from	influences	1029:1038	arg1	properties					1054:1063	hemostatic properties	1043:1063	hemostatic properties	1043:1063	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	5	24	theme	hemostatic	1043:1052	arg1	properties					1054:1063	hemostatic properties	1043:1063	hemostatic properties	1043:1063	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	7	25	theme	cell	1355:1358	arg1	lines					1360:1364	two cell lines	1351:1364	two cell lines	1351:1364	Moreover, the cytotoxicity and genotoxicity of the materials against two cell lines, human BJ fibroblasts and human KERTr keratinocytes, was investigated.					
34768816	1	26	theme	Marine	115:120	arg1	polysaccharides					122:136	Marine polysaccharides	115:136	Marine polysaccharides	115:136	Marine polysaccharides are believed to be promising wound-dressing nanomaterials because of their biocompatibility, antibacterial and hemostatic activity, and ability to easily shape into transparent films, hydrogels, and porous foams that can provide a moist micro-environment and adsorb exudates.					
34768816	4	27	used	used	920:923	arg2	fillers					928:934	fillers	928:934	fillers	928:934	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	27	used	used	920:923	arg2	cellulose					904:912	native and phosphorylated cellulose	878:912	native and phosphorylated cellulose	878:912	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	27	used	used	920:923	arg2	cellulose					856:864	microcrystalline and nano-sized cellulose	824:864	cellulose	856:864	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	1	28	theme	antibacterial	231:243	arg1	activity					260:267	antibacterial and hemostatic activity	231:267	antibacterial and hemostatic activity	231:267	Marine polysaccharides are believed to be promising wound-dressing nanomaterials because of their biocompatibility, antibacterial and hemostatic activity, and ability to easily shape into transparent films, hydrogels, and porous foams that can provide a moist micro-environment and adsorb exudates.					
34768816	2	29	theme	nanomaterials	500:512	arg1	preparation					456:466	the preparation	452:466	the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems	452:631	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	11	30	theme	biocompatible	1919:1931	arg1	films					1933:1937	These biocompatible films	1913:1937	These biocompatible films	1913:1937	These biocompatible films showed no genotoxicity, and very importantly for wound healing, most of them significantly accelerated migration of both fibroblasts and keratinocytes.					
34768816	9	31	theme	ROS	1793:1795	arg1	level					1784:1788	the level	1780:1788	the level of ROS	1780:1795	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	32	theme	reactive	1708:1715	arg1	ROS					1733:1735	ROS	1733:1735	ROS	1733:1735	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	32	theme	reactive	1708:1715	arg1	species					1724:1730	reactive oxygen species	1708:1730	reactive oxygen species (ROS)	1708:1736	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	7	33	dep	cytotoxicity	1296:1307	arg1	the					1292:1294	the	1292:1294	the	1292:1294	Moreover, the cytotoxicity and genotoxicity of the materials against two cell lines, human BJ fibroblasts and human KERTr keratinocytes, was investigated.					
34768816	2	34	theme	polysaccharide-derived	477:498	arg1	nanomaterials					500:512	novel polysaccharide-derived nanomaterials	471:512	novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems	471:631	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	11	35	theme	keratinocytes	2076:2088	arg1	migration					2042:2050	migration	2042:2050	migration of both fibroblasts and keratinocytes	2042:2088	These biocompatible films showed no genotoxicity, and very importantly for wound healing, most of them significantly accelerated migration of both fibroblasts and keratinocytes.					
34768816	9	36	theme	Potential	1551:1559	arg1	mechanisms					1561:1570	Potential mechanisms	1551:1570	Potential mechanisms of cytotoxicity	1551:1586	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	37	theme	oxygen	1717:1722	arg1	ROS					1733:1735	ROS	1733:1735	ROS	1733:1735	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	37	theme	oxygen	1717:1722	arg1	species					1724:1730	reactive oxygen species	1708:1730	reactive oxygen species (ROS)	1708:1736	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	1	38	theme	hemostatic	249:258	arg1	activity					260:267	antibacterial and hemostatic activity	231:267	antibacterial and hemostatic activity	231:267	Marine polysaccharides are believed to be promising wound-dressing nanomaterials because of their biocompatibility, antibacterial and hemostatic activity, and ability to easily shape into transparent films, hydrogels, and porous foams that can provide a moist micro-environment and adsorb exudates.					
34768816	2	39	theme	novel	471:475	arg1	nanomaterials					500:512	novel polysaccharide-derived nanomaterials	471:512	novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems	471:631	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	2	40	theme	systems	625:631	arg1	requirements					589:600	the mechanical and biological requirements	559:600	the mechanical and biological requirements of ideal wound healing systems	559:631	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	3	41	theme	films	747:751	arg1	characteristics					676:690	the characteristics	672:690	the characteristics of six different cellulose-filled chitosan transparent films as potential factors that could help to accelerate wound healing	672:816	In this contribution, we investigated the characteristics of six different cellulose-filled chitosan transparent films as potential factors that could help to accelerate wound healing.					
34768816	2	42	theme	chemical	534:541	arg1	objects					543:549	chemical objects	534:549	chemical objects	534:549	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	9	43	theme	microcellulose-filled	1637:1657	arg1	compounds					1682:1690	the compounds	1678:1690	the compounds	1678:1690	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	43	theme	microcellulose-filled	1637:1657	arg1	films					1668:1672	phosphorylated microcellulose-filled chitosan films	1622:1672	phosphorylated microcellulose-filled chitosan films	1622:1672	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	43	theme	microcellulose-filled	1637:1657	arg1	one					1617:1619	one	1617:1619	one	1617:1619	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	1	44	theme	porous	337:342	arg1	foams					344:348	porous foams	337:348	porous foams	337:348	Marine polysaccharides are believed to be promising wound-dressing nanomaterials because of their biocompatibility, antibacterial and hemostatic activity, and ability to easily shape into transparent films, hydrogels, and porous foams that can provide a moist micro-environment and adsorb exudates.					
34768816	2	45	theme	healing	617:623	arg1	systems					625:631	ideal wound healing systems	605:631	ideal wound healing systems	605:631	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	5	46	theme	coagulation	1197:1207	arg1	system					1209:1214	the blood coagulation system	1187:1214	the blood coagulation system	1187:1214	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	9	47	theme	cytotoxicity	1575:1586	arg1	mechanisms					1561:1570	Potential mechanisms	1551:1570	Potential mechanisms of cytotoxicity	1551:1586	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	0	48	theme	Wound	33:37	arg1	Healing					39:45	Wound Healing	33:45	Wound Healing	33:45	Insight into Factors Influencing Wound Healing Using Phosphorylated Cellulose-Filled-Chitosan Nanocomposite Films.					
34768816	11	49	theme	wound	1988:1992	arg1	healing					1994:2000	wound healing	1988:2000	wound healing	1988:2000	These biocompatible films showed no genotoxicity, and very importantly for wound healing, most of them significantly accelerated migration of both fibroblasts and keratinocytes.					
34768816	2	50	theme	wound	611:615	arg1	healing					617:623	ideal wound healing	605:623	ideal wound healing systems	605:631	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	3	51	theme	chitosan	726:733	arg1	films					747:751	six different cellulose-filled chitosan transparent films	695:751	six different cellulose-filled chitosan transparent films	695:751	In this contribution, we investigated the characteristics of six different cellulose-filled chitosan transparent films as potential factors that could help to accelerate wound healing.					
34768816	9	52	dep	tested	1692:1697	arg1	produced					1699:1706	produced	1699:1706	tested produced reactive oxygen species (ROS) to a small extent	1692:1754	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	0	53	theme	Phosphorylated	53:66	arg1	Films					108:112	Phosphorylated Cellulose-Filled-Chitosan Nanocomposite Films	53:112	Phosphorylated Cellulose-Filled-Chitosan Nanocomposite Films	53:112	Insight into Factors Influencing Wound Healing Using Phosphorylated Cellulose-Filled-Chitosan Nanocomposite Films.					
34768816	7	54	theme	materials	1333:1341	arg1	cytotoxicity					1296:1307	cytotoxicity	1296:1307	cytotoxicity	1296:1307	Moreover, the cytotoxicity and genotoxicity of the materials against two cell lines, human BJ fibroblasts and human KERTr keratinocytes, was investigated.					
34768816	7	54	theme	materials	1333:1341	arg1	genotoxicity					1313:1324	genotoxicity	1313:1324	genotoxicity	1313:1324	Moreover, the cytotoxicity and genotoxicity of the materials against two cell lines, human BJ fibroblasts and human KERTr keratinocytes, was investigated.					
34768816	0	55	theme	Cellulose-Filled-Chitosan	68:92	arg1	Films					108:112	Phosphorylated Cellulose-Filled-Chitosan Nanocomposite Films	53:112	Phosphorylated Cellulose-Filled-Chitosan Nanocomposite Films	53:112	Insight into Factors Influencing Wound Healing Using Phosphorylated Cellulose-Filled-Chitosan Nanocomposite Films.					
34768816	5	56	theme	extrinsic	1151:1159	arg1	pathways					1175:1182	both the extrinsic and intrinsic pathways	1142:1182	pathways	1175:1182	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	6	57	theme	antioxidant	1260:1270	arg1	capacity					1272:1279	antioxidant capacity	1260:1279	antioxidant capacity	1260:1279	We also showed that all biocomposites have antioxidant capacity.					
34768816	9	58	theme	phosphorylated	1622:1635	arg1	compounds					1682:1690	the compounds	1678:1690	the compounds	1678:1690	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	58	theme	phosphorylated	1622:1635	arg1	films					1668:1672	phosphorylated microcellulose-filled chitosan films	1622:1672	phosphorylated microcellulose-filled chitosan films	1622:1672	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	58	theme	phosphorylated	1622:1635	arg1	one					1617:1619	one	1617:1619	one	1617:1619	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	4	59	theme	functionalization	986:1002	arg1	roles					968:972	the roles	964:972	the roles of size and functionalization	964:1002	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	5	60	theme	intrinsic	1165:1173	arg1	pathways					1175:1182	both the extrinsic and intrinsic pathways	1142:1182	pathways	1175:1182	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	10	61	theme	transmembrane	1848:1860	arg1	potential					1876:1884	The transmembrane mitochondrial potential	1844:1884	The transmembrane mitochondrial potential	1844:1884	The transmembrane mitochondrial potential was very slightly lowered.					
34768816	4	62	dep	cellulose	856:864	arg1	Both					819:822	Both	819:822	Both	819:822	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	8	63	theme	low	1540:1542	arg1	level					1544:1548	a relatively low level	1527:1548	a relatively low level	1527:1548	The nature of the cellulose used as a filler was found to influence their cytotoxicity at a relatively low level.					
34768816	6	64	contain	have	1255:1258	arg1	biocomposites					1241:1253	all biocomposites	1237:1253	all biocomposites	1237:1253	We also showed that all biocomposites have antioxidant capacity.					
34768816	6	64	contain	have	1255:1258	arg2	capacity					1272:1279	antioxidant capacity	1260:1279	antioxidant capacity	1260:1279	We also showed that all biocomposites have antioxidant capacity.					
34768816	9	65	theme	antioxidant	1820:1830	arg1	properties					1832:1841	their antioxidant properties	1814:1841	their antioxidant properties	1814:1841	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	4	66	theme	phosphorylated	889:902	arg1	cellulose					904:912	native and phosphorylated cellulose	878:912	native and phosphorylated cellulose	878:912	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	66	theme	phosphorylated	889:902	arg1	cellulose					856:864	microcrystalline and nano-sized cellulose	824:864	cellulose	856:864	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	66	theme	phosphorylated	889:902	arg1	fillers					928:934	fillers	928:934	fillers	928:934	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	3	67	theme	different	699:707	arg1	films					747:751	six different cellulose-filled chitosan transparent films	695:751	six different cellulose-filled chitosan transparent films	695:751	In this contribution, we investigated the characteristics of six different cellulose-filled chitosan transparent films as potential factors that could help to accelerate wound healing.					
34768816	1	68	theme	promising	157:165	arg1	nanomaterials					182:194	promising wound-dressing nanomaterials	157:194	promising wound-dressing nanomaterials	157:194	Marine polysaccharides are believed to be promising wound-dressing nanomaterials because of their biocompatibility, antibacterial and hemostatic activity, and ability to easily shape into transparent films, hydrogels, and porous foams that can provide a moist micro-environment and adsorb exudates.					
34768816	3	69	theme	potential	756:764	arg1	factors					766:772	potential factors	756:772	potential factors that could help to accelerate wound healing	756:816	In this contribution, we investigated the characteristics of six different cellulose-filled chitosan transparent films as potential factors that could help to accelerate wound healing.					
34768816	3	70	theme	cellulose-filled	709:724	arg1	films					747:751	six different cellulose-filled chitosan transparent films	695:751	six different cellulose-filled chitosan transparent films	695:751	In this contribution, we investigated the characteristics of six different cellulose-filled chitosan transparent films as potential factors that could help to accelerate wound healing.					
34768816	1	71	theme	wound-dressing	167:180	arg1	nanomaterials					182:194	promising wound-dressing nanomaterials	157:194	promising wound-dressing nanomaterials	157:194	Marine polysaccharides are believed to be promising wound-dressing nanomaterials because of their biocompatibility, antibacterial and hemostatic activity, and ability to easily shape into transparent films, hydrogels, and porous foams that can provide a moist micro-environment and adsorb exudates.					
34768816	4	72	theme	microcrystalline	824:839	arg1	cellulose					856:864	microcrystalline and nano-sized cellulose	824:864	cellulose	856:864	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	72	theme	microcrystalline	824:839	arg1	cellulose					904:912	native and phosphorylated cellulose	878:912	native and phosphorylated cellulose	878:912	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	4	72	theme	microcrystalline	824:839	arg1	fillers					928:934	fillers	928:934	fillers	928:934	Both microcrystalline and nano-sized cellulose, as well as native and phosphorylated cellulose, were used as fillers to simultaneously elucidate the roles of size and functionalization.					
34768816	0	73	theme	Nanocomposite	94:106	arg1	Films					108:112	Phosphorylated Cellulose-Filled-Chitosan Nanocomposite Films	53:112	Phosphorylated Cellulose-Filled-Chitosan Nanocomposite Films	53:112	Insight into Factors Influencing Wound Healing Using Phosphorylated Cellulose-Filled-Chitosan Nanocomposite Films.					
34768816	2	74	theme	Current	414:420	arg1	efforts					422:428	Current efforts	414:428	Current efforts	414:428	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	5	75	from	properties	1054:1063	arg1	assessment					1009:1018	The assessment	1005:1018	The assessment of their influences on hemostatic properties	1005:1063	The assessment of their influences on hemostatic properties indicated that the tested nanocomposites shorten clotting times by affecting both the extrinsic and intrinsic pathways of the blood coagulation system.					
34768816	2	76	theme	mechanical	563:572	arg1	requirements					589:600	the mechanical and biological requirements	559:600	the mechanical and biological requirements of ideal wound healing systems	559:631	Current efforts are firmly focused on the preparation of novel polysaccharide-derived nanomaterials functionalized with chemical objects to meet the mechanical and biological requirements of ideal wound healing systems.					
34768816	7	77	theme	human	1392:1396	arg1	keratinocytes					1404:1416	human KERTr keratinocytes	1392:1416	human KERTr keratinocytes	1392:1416	Moreover, the cytotoxicity and genotoxicity of the materials against two cell lines, human BJ fibroblasts and human KERTr keratinocytes, was investigated.					
34768816	9	78	theme	chitosan	1659:1666	arg1	compounds					1682:1690	the compounds	1678:1690	the compounds	1678:1690	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	78	theme	chitosan	1659:1666	arg1	films					1668:1672	phosphorylated microcellulose-filled chitosan films	1622:1672	phosphorylated microcellulose-filled chitosan films	1622:1672	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	78	theme	chitosan	1659:1666	arg1	one					1617:1619	one	1617:1619	one	1617:1619	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34768816	9	79	theme	small	1743:1747	arg1	extent					1749:1754	a small extent	1741:1754	a small extent	1741:1754	Potential mechanisms of cytotoxicity were also investigated; only one (phosphorylated microcellulose-filled chitosan films) of the compounds tested produced reactive oxygen species (ROS) to a small extent, and some films reduced the level of ROS, probably due to their antioxidant properties.					
34246313	6	0	theme	overlapping	946:956	arg1	features					967:974	overlapping clinical features	946:974	overlapping clinical features harbouring the homozygous missense variant A391T	946:1023	Furthermore, we describe a third patient with overlapping clinical features harbouring the homozygous missense variant A391T.					
34246313	10	1	theme	Serum	1460:1464	arg1	transferrin					1466:1476	Serum transferrin isoelectric focusing (IEF)	1460:1503	Serum transferrin isoelectric focusing (IEF)	1460:1503	Serum transferrin isoelectric focusing (IEF) yielded variable results with slight increase of trisialotransferrin isoforms or even normal pattern.					
34246313	17	2	theme	common	2718:2723	arg1	variant					2734:2740	the common missense variant	2714:2740	the common missense variant	2714:2740	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	1	3	theme	glycosylation	234:246	arg1	SLC39A8					75:81	BACKGROUND SLC39A8	64:81	BACKGROUND SLC39A8	64:81	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	1	3	theme	glycosylation	234:246	arg1	disorder					222:229	a type 2 congenital disorder	202:229	a type 2 congenital disorder of glycosylation (CDG)	202:252	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	12	4	theme	undergalactosylated	1862:1880	arg1	precursors					1902:1911	undergalactosylated and undersialylated precursors	1862:1911	undergalactosylated and undersialylated precursors	1862:1911	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	12	4	theme	undergalactosylated	1862:1880	arg1	A2G1S1					2010:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	8	5	theme	basal	1306:1310	arg1	ganglia					1312:1318	the basal ganglia	1302:1318	the basal ganglia	1302:1318	Neuroimaging showed a Leigh syndrome-like pattern involving the basal ganglia, thalami and white matter.					
34246313	11	6	theme	transferrin	1658:1668	arg1	N-glycans					1670:1678	hypogalactosylated transferrin N-glycans	1639:1678	hypogalactosylated transferrin N-glycans	1639:1678	MALDI-MS showed the presence of hypogalactosylated transferrin N-glycans, spontaneously decreasing during the disease course, only in one affected sibling.					
34246313	3	7	theme	glycosylation	529:541	arg1	profile					543:549	variable transferrin glycosylation profile	508:549	variable transferrin glycosylation profile	508:549	Patients with SLC39A8-CDG present with different clinical and neuroradiological features linked to variable transferrin glycosylation profile.					
34246313	16	8	from	patients	2590:2597	arg1	diagnosis					2533:2541	diagnosis	2533:2541	diagnosis	2533:2541	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	16	8	from	patients	2590:2597	arg1	management					2556:2565	clinical management	2547:2565	clinical management	2547:2565	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	5	9	theme	clinical manifestations	691:713	arg1	features					734:741	clinical manifestations, neuroradiological features	691:741	clinical manifestations, neuroradiological features	691:741	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	16	10	theme	therapy	2570:2576	arg1	response					2578:2585	therapy response	2570:2585	therapy response in patients with SLC39A8-CDG	2570:2614	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	2	11	theme	SLC39A8	266:272	arg1	variant					283:289	The common SLC39A8 missense variant	255:289	The common SLC39A8 missense variant A391T	255:295	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	2	11	theme	SLC39A8	266:272	arg1	A391T					291:295	A391T	291:295	A391T	291:295	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	16	12	from	response	2578:2585	arg1	patients					2590:2597	patients	2590:2597	patients with SLC39A8-CDG	2590:2614	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	17	13	theme	clinical	2811:2818	arg1	features					2855:2862	clinical, biochemical and neuroradiological features	2811:2862	clinical, biochemical and neuroradiological features	2811:2862	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	17	14	from	influence	2673:2681	arg1	expression					2750:2759	expression	2750:2759	expression	2750:2759	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	17	14	from	influence	2673:2681	arg1	impact					2801:2806	their impact	2795:2806	their impact on clinical, biochemical and neuroradiological features	2795:2862	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	17	14	from	influence	2673:2681	arg1	function					2765:2772	function	2765:2772	function	2765:2772	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	4	15	theme	treated	649:655	arg1	patients					657:664	treated patients	649:664	treated patients	649:664	Galactose and Mn supplementation therapy results in the biochemical and clinical amelioration of treated patients.					
34246313	7	16	theme	postural	1134:1141	arg1	pattern					1143:1149	dystonic postural pattern	1125:1149	dystonic postural pattern	1125:1149	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	8	17	theme	syndrome-like	1270:1282	arg1	pattern					1284:1290	a Leigh syndrome-like pattern	1262:1290	a Leigh syndrome-like pattern involving the basal ganglia, thalami and white matter	1262:1344	Neuroimaging showed a Leigh syndrome-like pattern involving the basal ganglia, thalami and white matter.					
34246313	6	18	theme	missense	1002:1009	arg1	A391T					1019:1023	the homozygous missense variant A391T	987:1023	the homozygous missense variant A391T	987:1023	Furthermore, we describe a third patient with overlapping clinical features harbouring the homozygous missense variant A391T.					
34246313	17	19	theme	biochemical	2821:2831	arg1	features					2855:2862	clinical, biochemical and neuroradiological features	2811:2862	clinical, biochemical and neuroradiological features	2811:2862	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	7	20	theme	patients	1062:1069	arg1	phenotype					1039:1047	The clinical phenotype	1026:1047	The clinical phenotype of the three patients	1026:1069	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	5	21	theme	Italian	884:890	arg1	family					892:897	the same Italian family	875:897	the same Italian family	875:897	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	17	22	theme	neuroradiological	2837:2853	arg1	features					2855:2862	clinical, biochemical and neuroradiological features	2811:2862	clinical, biochemical and neuroradiological features	2811:2862	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	17	23	theme	ZIP8	2777:2780	arg1	protein					2782:2788	ZIP8 protein	2777:2788	ZIP8 protein	2777:2788	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	7	24	theme	developmental	1099:1111	arg1	disability					1113:1122	severe developmental disability	1092:1122	severe developmental disability	1092:1122	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	16	25	from	diagnosis	2533:2541	arg1	patients					2590:2597	patients	2590:2597	patients with SLC39A8-CDG	2590:2614	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	15	26	from	SLC39A8-CDG	2317:2327	arg1	association					2332:2342	association	2332:2342	association with the homozygous A391T missense variant	2332:2385	We show, for the first time, a severe neurological phenotype overlapping with that described for SLC39A8-CDG in association with the homozygous A391T missense variant.					
34246313	3	27	with	Patients	409:416	arg1	SLC39A8-CDG					423:433	SLC39A8-CDG	423:433	SLC39A8-CDG	423:433	Patients with SLC39A8-CDG present with different clinical and neuroradiological features linked to variable transferrin glycosylation profile.					
34246313	16	28	theme	MS-based	2482:2489	arg1	glycomics					2497:2505	MS-based serum glycomics	2482:2505	MS-based serum glycomics	2482:2505	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	13	29	with	consistent	2087:2096	arg1	SLC39A8-CDG					2103:2113	SLC39A8-CDG	2103:2113	SLC39A8-CDG	2103:2113	CONCLUSIONS Clinical, MRI and glycosylation features of patients are consistent with SLC39A8-CDG.					
34246313	10	30	theme	variable	1513:1520	arg1	results					1522:1528	variable results	1513:1528	variable results	1513:1528	Serum transferrin isoelectric focusing (IEF) yielded variable results with slight increase of trisialotransferrin isoforms or even normal pattern.					
34246313	16	31	theme	glycomics	2497:2505	arg1	efficacy					2470:2477	the efficacy	2466:2477	the efficacy of MS-based serum glycomics	2466:2505	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	15	32	theme	A391T	2364:2368	arg1	variant					2379:2385	the homozygous A391T missense variant	2349:2385	the homozygous A391T missense variant	2349:2385	We show, for the first time, a severe neurological phenotype overlapping with that described for SLC39A8-CDG in association with the homozygous A391T missense variant.					
34246313	12	33	theme	distinct	1796:1803	arg1	pattern					1805:1811	a distinct pattern	1794:1811	a distinct pattern for the three patients	1794:1834	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	16	34	theme	auxiliary	2510:2518	arg1	tool					2520:2523	auxiliary tool	2510:2523	auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG	2510:2614	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	5	35	theme	novel	779:783	arg1	variants					793:800	novel SLC39A8 variants	779:800	novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family	779:897	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	1	36	theme	BACKGROUND	64:73	arg1	SLC39A8					75:81	BACKGROUND SLC39A8	64:81	BACKGROUND SLC39A8	64:81	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	1	36	theme	BACKGROUND	64:73	arg1	gene					86:89	a gene	84:89	a gene located on chromosome 4q24	84:116	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	1	36	theme	BACKGROUND	64:73	arg1	disorder					222:229	a type 2 congenital disorder	202:229	a type 2 congenital disorder of glycosylation (CDG)	202:252	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	16	37	theme	serum	2491:2495	arg1	glycomics					2497:2505	MS-based serum glycomics	2482:2505	MS-based serum glycomics	2482:2505	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	2	38	theme	systemic	361:368	arg1	disorders					370:378	multiple neurological and systemic disorders	335:378	multiple neurological and systemic disorders	335:378	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	9	39	theme	atrophic	1368:1375	arg1	changes					1401:1407	atrophic cerebral and cerebellum changes	1368:1407	atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG	1368:1435	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	9	39	theme	atrophic	1368:1375	arg1	well					1454:1457	well	1454:1457	well	1454:1457	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	14	40	theme	syndrome-like	2169:2181	arg1	presentation					2191:2202	Leigh syndrome-like disease presentation	2163:2202	Leigh syndrome-like disease presentation of SLC39A8-CDG	2163:2217	We document two novel variants associated with Leigh syndrome-like disease presentation of SLC39A8-CDG.					
34246313	12	41	theme	serum	1769:1773	arg1	N-glycome					1775:1783	Total serum N-glycome	1763:1783	Total serum N-glycome	1763:1783	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	2	42	theme	neurological	344:355	arg1	disorders					370:378	multiple neurological and systemic disorders	335:378	multiple neurological and systemic disorders	335:378	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	5	43	dep	RESULTS	667:673	arg1	report					684:689	report	684:689	report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family	684:897	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	4	44	theme	Galactose	552:560	arg1	therapy					585:591	Galactose and Mn supplementation therapy	552:591	Galactose and Mn supplementation therapy	552:591	Galactose and Mn supplementation therapy results in the biochemical and clinical amelioration of treated patients.					
34246313	16	45	theme	N-glycome	2442:2450	arg1	amelioration					2414:2425	a spontaneous amelioration	2400:2425	a spontaneous amelioration of transferrin N-glycome	2400:2450	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	2	46	theme	decreased	389:397	arg1	Mn					405:406	decreased serum Mn	389:406	decreased serum Mn	389:406	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	4	47	theme	Mn	566:567	arg1	therapy					585:591	Galactose and Mn supplementation therapy	552:591	Galactose and Mn supplementation therapy	552:591	Galactose and Mn supplementation therapy results in the biochemical and clinical amelioration of treated patients.					
34246313	10	48	theme	trisialotransferrin	1554:1572	arg1	isoforms					1574:1581	trisialotransferrin isoforms	1554:1581	trisialotransferrin isoforms	1554:1581	Serum transferrin isoelectric focusing (IEF) yielded variable results with slight increase of trisialotransferrin isoforms or even normal pattern.					
34246313	12	49	theme	biantennary	1933:1943	arg1	glycans					1945:1951	fully sialylated biantennary glycans	1916:1951	fully sialylated biantennary glycans	1916:1951	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	16	50	theme	response	2578:2585	arg1	diagnosis					2533:2541	diagnosis	2533:2541	diagnosis	2533:2541	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	16	50	theme	response	2578:2585	arg1	management					2556:2565	clinical management	2547:2565	clinical management	2547:2565	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	3	51	attach	linked	498:503	arg1	profile					543:549	variable transferrin glycosylation profile	508:549	variable transferrin glycosylation profile	508:549	Patients with SLC39A8-CDG present with different clinical and neuroradiological features linked to variable transferrin glycosylation profile.					
34246313	3	51	attach	linked	498:503	arg2	features					489:496	different clinical and neuroradiological features	448:496	different clinical and neuroradiological features linked to variable transferrin glycosylation profile	448:549	Patients with SLC39A8-CDG present with different clinical and neuroradiological features linked to variable transferrin glycosylation profile.					
34246313	6	52	with	patient	933:939	arg1	features					967:974	overlapping clinical features	946:974	overlapping clinical features harbouring the homozygous missense variant A391T	946:1023	Furthermore, we describe a third patient with overlapping clinical features harbouring the homozygous missense variant A391T.					
34246313	7	53	from	progression	1185:1195	arg1	siblings					1232:1239	the two affected siblings	1215:1239	the two affected siblings	1215:1239	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	10	54	theme	normal	1591:1596	arg1	pattern					1598:1604	even normal pattern	1586:1604	even normal pattern	1586:1604	Serum transferrin isoelectric focusing (IEF) yielded variable results with slight increase of trisialotransferrin isoforms or even normal pattern.					
34246313	3	55	theme	different	448:456	arg1	features					489:496	different clinical and neuroradiological features	448:496	different clinical and neuroradiological features linked to variable transferrin glycosylation profile	448:549	Patients with SLC39A8-CDG present with different clinical and neuroradiological features linked to variable transferrin glycosylation profile.					
34246313	10	56	dep	transferrin	1466:1476	arg1	isoelectric					1478:1488	isoelectric	1478:1488	isoelectric	1478:1488	Serum transferrin isoelectric focusing (IEF) yielded variable results with slight increase of trisialotransferrin isoforms or even normal pattern.					
34246313	10	56	dep	transferrin	1466:1476	arg1	IEF					1500:1502	IEF	1500:1502	IEF	1500:1502	Serum transferrin isoelectric focusing (IEF) yielded variable results with slight increase of trisialotransferrin isoforms or even normal pattern.					
34246313	12	57	theme	monosialo-monogalacto-biantennary	1968:2000	arg1	A2G1S1					2010:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	15	58	theme	severe	2251:2256	arg1	phenotype					2271:2279	a severe neurological phenotype	2249:2279	a severe neurological phenotype overlapping with that described for SLC39A8-CDG in association with the homozygous A391T missense variant	2249:2385	We show, for the first time, a severe neurological phenotype overlapping with that described for SLC39A8-CDG in association with the homozygous A391T missense variant.					
34246313	12	59	theme	precursors	1902:1911	arg1	levels					1852:1857	increased levels	1842:1857	increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1	1842:2015	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	17	60	from	expression	2750:2759	arg1	features					2855:2862	clinical, biochemical and neuroradiological features	2811:2862	clinical, biochemical and neuroradiological features	2811:2862	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	17	61	theme	SLC39A8	2686:2692	arg1	variants					2694:2701	SLC39A8 variants	2686:2701	SLC39A8 variants	2686:2701	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	17	61	theme	SLC39A8	2686:2692	arg1	variant					2734:2740	the common missense variant	2714:2740	the common missense variant	2714:2740	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	4	62	theme	biochemical	608:618	arg1	amelioration					633:644	the biochemical and clinical amelioration	604:644	the biochemical and clinical amelioration of treated patients	604:664	Galactose and Mn supplementation therapy results in the biochemical and clinical amelioration of treated patients.					
34246313	12	63	gly	sialylated	1922:1931	arg1	glycans					1945:1951	fully sialylated biantennary glycans	1916:1951	fully sialylated biantennary glycans	1916:1951	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	1	64	theme	type	204:207	arg1	SLC39A8					75:81	BACKGROUND SLC39A8	64:81	BACKGROUND SLC39A8	64:81	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	1	64	theme	type	204:207	arg1	disorder					222:229	a type 2 congenital disorder	202:229	a type 2 congenital disorder of glycosylation (CDG)	202:252	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	6	65	theme	clinical	958:965	arg1	features					967:974	overlapping clinical features	946:974	overlapping clinical features harbouring the homozygous missense variant A391T	946:1023	Furthermore, we describe a third patient with overlapping clinical features harbouring the homozygous missense variant A391T.					
34246313	4	66	theme	clinical	624:631	arg1	amelioration					633:644	the biochemical and clinical amelioration	604:644	the biochemical and clinical amelioration of treated patients	604:664	Galactose and Mn supplementation therapy results in the biochemical and clinical amelioration of treated patients.					
34246313	11	67	theme	affected	1745:1752	arg1	sibling					1754:1760	one affected sibling	1741:1760	one affected sibling	1741:1760	MALDI-MS showed the presence of hypogalactosylated transferrin N-glycans, spontaneously decreasing during the disease course, only in one affected sibling.					
34246313	7	68	theme	severe	1178:1183	arg1	progression					1185:1195	a more severe progression	1171:1195	a more severe progression of the disease in the two affected siblings	1171:1239	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	11	69	theme	disease	1717:1723	arg1	course					1725:1730	the disease course	1713:1730	the disease course	1713:1730	MALDI-MS showed the presence of hypogalactosylated transferrin N-glycans, spontaneously decreasing during the disease course, only in one affected sibling.					
34246313	2	70	theme	increased	316:324	arg1	risk					326:329	increased risk	316:329	increased risk for multiple neurological and systemic disorders	316:378	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	1	71	theme	congenital	211:220	arg1	SLC39A8					75:81	BACKGROUND SLC39A8	64:81	BACKGROUND SLC39A8	64:81	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	1	71	theme	congenital	211:220	arg1	disorder					222:229	a type 2 congenital disorder	202:229	a type 2 congenital disorder of glycosylation (CDG)	202:252	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	12	72	gly	undersialylated	1886:1900	arg1	precursors					1902:1911	undergalactosylated and undersialylated precursors	1862:1911	undergalactosylated and undersialylated precursors	1862:1911	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	12	72	gly	undersialylated	1886:1900	arg1	A2G1S1					2010:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	11	73	theme	N-glycans	1670:1678	arg1	presence					1627:1634	the presence	1623:1634	the presence of hypogalactosylated transferrin N-glycans	1623:1678	MALDI-MS showed the presence of hypogalactosylated transferrin N-glycans, spontaneously decreasing during the disease course, only in one affected sibling.					
34246313	6	74	theme	third	927:931	arg1	patient					933:939	a third patient	925:939	a third patient with overlapping clinical features harbouring the homozygous missense variant A391T	925:1023	Furthermore, we describe a third patient with overlapping clinical features harbouring the homozygous missense variant A391T.					
34246313	13	75	dep	Clinical	2030:2037	arg1	CONCLUSIONS					2018:2028	CONCLUSIONS	2018:2028	CONCLUSIONS	2018:2028	CONCLUSIONS Clinical, MRI and glycosylation features of patients are consistent with SLC39A8-CDG.					
34246313	13	75	dep	Clinical	2030:2037	arg1	features					2062:2069	features	2062:2069	features	2062:2069	CONCLUSIONS Clinical, MRI and glycosylation features of patients are consistent with SLC39A8-CDG.					
34246313	15	76	theme	homozygous	2353:2362	arg1	variant					2379:2385	the homozygous A391T missense variant	2349:2385	the homozygous A391T missense variant	2349:2385	We show, for the first time, a severe neurological phenotype overlapping with that described for SLC39A8-CDG in association with the homozygous A391T missense variant.					
34246313	3	77	theme	transferrin	517:527	arg1	profile					543:549	variable transferrin glycosylation profile	508:549	variable transferrin glycosylation profile	508:549	Patients with SLC39A8-CDG present with different clinical and neuroradiological features linked to variable transferrin glycosylation profile.					
34246313	11	78	from	course	1725:1730	arg1	sibling					1754:1760	one affected sibling	1741:1760	one affected sibling	1741:1760	MALDI-MS showed the presence of hypogalactosylated transferrin N-glycans, spontaneously decreasing during the disease course, only in one affected sibling.					
34246313	5	79	theme	family	892:897	arg1	siblings					863:870	two siblings	859:870	two siblings of the same Italian family	859:897	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	9	80	with	consistent	1409:1418	arg1	SLC39A8-CDG					1425:1435	SLC39A8-CDG	1425:1435	SLC39A8-CDG	1425:1435	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	2	81	theme	missense	274:281	arg1	variant					283:289	The common SLC39A8 missense variant	255:289	The common SLC39A8 missense variant A391T	255:295	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	2	81	theme	missense	274:281	arg1	A391T					291:295	A391T	291:295	A391T	291:295	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	15	82	with	association	2332:2342	arg1	variant					2379:2385	the homozygous A391T missense variant	2349:2385	the homozygous A391T missense variant	2349:2385	We show, for the first time, a severe neurological phenotype overlapping with that described for SLC39A8-CDG in association with the homozygous A391T missense variant.					
34246313	1	83	theme	transporter	150:160	arg1	ZIP8					162:165	the manganese (Mn) transporter ZIP8	131:165	the manganese (Mn) transporter ZIP8	131:165	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	16	84	with	patients	2590:2597	arg1	SLC39A8-CDG					2604:2614	SLC39A8-CDG	2604:2614	SLC39A8-CDG	2604:2614	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	12	85	theme	increased	1842:1850	arg1	levels					1852:1857	increased levels	1842:1857	increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1	1842:2015	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	7	86	theme	dystonic	1125:1132	arg1	pattern					1143:1149	dystonic postural pattern	1125:1149	dystonic postural pattern	1125:1149	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	11	87	theme	hypogalactosylated	1639:1656	arg1	N-glycans					1670:1678	hypogalactosylated transferrin N-glycans	1639:1678	hypogalactosylated transferrin N-glycans	1639:1678	MALDI-MS showed the presence of hypogalactosylated transferrin N-glycans, spontaneously decreasing during the disease course, only in one affected sibling.					
34246313	2	88	theme	common	259:264	arg1	variant					283:289	The common SLC39A8 missense variant	255:289	The common SLC39A8 missense variant A391T	255:295	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	2	88	theme	common	259:264	arg1	A391T					291:295	A391T	291:295	A391T	291:295	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	6	89	theme	variant	1011:1017	arg1	A391T					1019:1023	the homozygous missense variant A391T	987:1023	the homozygous missense variant A391T	987:1023	Furthermore, we describe a third patient with overlapping clinical features harbouring the homozygous missense variant A391T.					
34246313	17	90	from	function	2765:2772	arg1	features					2855:2862	clinical, biochemical and neuroradiological features	2811:2862	clinical, biochemical and neuroradiological features	2811:2862	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	5	91	from	variants	793:800	arg1	siblings					863:870	two siblings	859:870	two siblings of the same Italian family	859:897	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	5	92	theme	same	879:882	arg1	family					892:897	the same Italian family	875:897	the same Italian family	875:897	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	8	93	theme	Leigh	1264:1268	arg1	pattern					1284:1290	a Leigh syndrome-like pattern	1262:1290	a Leigh syndrome-like pattern involving the basal ganglia, thalami and white matter	1262:1344	Neuroimaging showed a Leigh syndrome-like pattern involving the basal ganglia, thalami and white matter.					
34246313	6	94	theme	homozygous	991:1000	arg1	A391T					1019:1023	the homozygous missense variant A391T	987:1023	the homozygous missense variant A391T	987:1023	Furthermore, we describe a third patient with overlapping clinical features harbouring the homozygous missense variant A391T.					
34246313	5	95	dep	c.1048G > A	803:813	arg1	p.Ser44Trp					844:853	p.Ser44Trp	844:853	c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp	803:853	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	5	95	dep	c.1048G > A	803:813	arg1	c.131C > G					832:841	c.131C > G	832:841	c.131C > G	832:841	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	5	95	dep	c.1048G > A	803:813	arg1	p.Gly350Arg					816:826	p.Gly350Arg	816:826	p.Gly350Arg	816:826	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	4	96	theme	patients	657:664	arg1	amelioration					633:644	the biochemical and clinical amelioration	604:644	the biochemical and clinical amelioration of treated patients	604:664	Galactose and Mn supplementation therapy results in the biochemical and clinical amelioration of treated patients.					
34246313	7	97	theme	severe	1092:1097	arg1	disability					1113:1122	severe developmental disability	1092:1122	severe developmental disability	1092:1122	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	1	98	theme	chromosome	102:111	arg1	4q24					113:116	chromosome 4q24	102:116	chromosome 4q24	102:116	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	5	99	dep	variants	793:800	arg1	c.1048G > A					803:813	c.1048G > A	803:813	c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp	803:853	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	17	100	theme	protein	2782:2788	arg1	expression					2750:2759	expression	2750:2759	expression	2750:2759	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	17	100	theme	protein	2782:2788	arg1	impact					2801:2806	their impact	2795:2806	their impact on clinical, biochemical and neuroradiological features	2795:2862	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	17	100	theme	protein	2782:2788	arg1	function					2765:2772	function	2765:2772	function	2765:2772	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	13	101	theme	patients	2074:2081	arg1	Clinical					2030:2037	Clinical	2030:2037	Clinical	2030:2037	CONCLUSIONS Clinical, MRI and glycosylation features of patients are consistent with SLC39A8-CDG.					
34246313	17	102	dep	expression	2750:2759	arg1	the					2746:2748	the	2746:2748	the	2746:2748	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	15	103	theme	missense	2370:2377	arg1	variant					2379:2385	the homozygous A391T missense variant	2349:2385	the homozygous A391T missense variant	2349:2385	We show, for the first time, a severe neurological phenotype overlapping with that described for SLC39A8-CDG in association with the homozygous A391T missense variant.					
34246313	16	104	from	management	2556:2565	arg1	patients					2590:2597	patients	2590:2597	patients with SLC39A8-CDG	2590:2614	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	9	105	located	detected	1442:1449	arg2	well					1454:1457	well	1454:1457	well	1454:1457	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	9	105	located	detected	1442:1449	arg1	siblings					1358:1365	the two siblings	1350:1365	the two siblings	1350:1365	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	9	105	located	detected	1442:1449	arg2	changes					1401:1407	atrophic cerebral and cerebellum changes	1368:1407	atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG	1368:1435	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	7	106	theme	clinical	1030:1037	arg1	phenotype					1039:1047	The clinical phenotype	1026:1047	The clinical phenotype of the three patients	1026:1069	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	1	107	theme	manganese	135:143	arg1	ZIP8					162:165	the manganese (Mn) transporter ZIP8	131:165	the manganese (Mn) transporter ZIP8	131:165	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	17	108	theme	missense	2725:2732	arg1	variant					2734:2740	the common missense variant	2714:2740	the common missense variant	2714:2740	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	15	109	theme	first	2237:2241	arg1	time					2243:2246	the first time	2233:2246	the first time	2233:2246	We show, for the first time, a severe neurological phenotype overlapping with that described for SLC39A8-CDG in association with the homozygous A391T missense variant.					
34246313	5	110	theme	SLC39A8	785:791	arg1	variants					793:800	novel SLC39A8 variants	779:800	novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family	779:897	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	14	111	theme	SLC39A8-CDG	2207:2217	arg1	presentation					2191:2202	Leigh syndrome-like disease presentation	2163:2202	Leigh syndrome-like disease presentation of SLC39A8-CDG	2163:2217	We document two novel variants associated with Leigh syndrome-like disease presentation of SLC39A8-CDG.					
34246313	14	112	theme	disease	2183:2189	arg1	presentation					2191:2202	Leigh syndrome-like disease presentation	2163:2202	Leigh syndrome-like disease presentation of SLC39A8-CDG	2163:2217	We document two novel variants associated with Leigh syndrome-like disease presentation of SLC39A8-CDG.					
34246313	17	113	from	impact	2801:2806	arg1	features					2855:2862	clinical, biochemical and neuroradiological features	2811:2862	clinical, biochemical and neuroradiological features	2811:2862	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	5	114	dep	clinical manifestations	691:713	arg1	neuroradiological					716:732	neuroradiological	716:732	neuroradiological	716:732	RESULTS Here, we report clinical manifestations, neuroradiological features and glycophenotypes associated with novel SLC39A8 variants (c.1048G > A; p.Gly350Arg and c.131C > G; p.Ser44Trp) in two siblings of the same Italian family.					
34246313	0	115	from	insights	39:46	arg1	SLC39A8-CDG					51:61	SLC39A8-CDG	51:61	SLC39A8-CDG	51:61	Clinical, molecular and glycophenotype insights in SLC39A8-CDG.					
34246313	10	116	theme	slight	1535:1540	arg1	increase					1542:1549	slight increase	1535:1549	slight increase of trisialotransferrin isoforms or even normal pattern	1535:1604	Serum transferrin isoelectric focusing (IEF) yielded variable results with slight increase of trisialotransferrin isoforms or even normal pattern.					
34246313	16	117	theme	transferrin	2430:2440	arg1	N-glycome					2442:2450	transferrin N-glycome	2430:2450	transferrin N-glycome	2430:2450	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	16	118	theme	spontaneous	2402:2412	arg1	amelioration					2414:2425	a spontaneous amelioration	2400:2425	a spontaneous amelioration of transferrin N-glycome	2400:2450	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	7	119	theme	affected	1223:1230	arg1	siblings					1232:1239	the two affected siblings	1215:1239	the two affected siblings	1215:1239	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	9	120	theme	cerebral	1377:1384	arg1	changes					1401:1407	atrophic cerebral and cerebellum changes	1368:1407	atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG	1368:1435	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	9	120	theme	cerebral	1377:1384	arg1	well					1454:1457	well	1454:1457	well	1454:1457	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	16	121	dep	diagnosis	2533:2541	arg1	the					2529:2531	the	2529:2531	the	2529:2531	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	14	122	theme	Leigh	2163:2167	arg1	presentation					2191:2202	Leigh syndrome-like disease presentation	2163:2202	Leigh syndrome-like disease presentation of SLC39A8-CDG	2163:2217	We document two novel variants associated with Leigh syndrome-like disease presentation of SLC39A8-CDG.					
34246313	12	123	theme	Total	1763:1767	arg1	N-glycome					1775:1783	Total serum N-glycome	1763:1783	Total serum N-glycome	1763:1783	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	16	124	theme	clinical	2547:2554	arg1	management					2556:2565	clinical management	2547:2565	clinical management	2547:2565	We observed a spontaneous amelioration of transferrin N-glycome, highlighting the efficacy of MS-based serum glycomics as auxiliary tool for the diagnosis and clinical management of therapy response in patients with SLC39A8-CDG.					
34246313	2	125	theme	multiple	335:342	arg1	disorders					370:378	multiple neurological and systemic disorders	335:378	multiple neurological and systemic disorders	335:378	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	9	126	theme	cerebellum	1390:1399	arg1	changes					1401:1407	atrophic cerebral and cerebellum changes	1368:1407	atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG	1368:1435	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	9	126	theme	cerebellum	1390:1399	arg1	well					1454:1457	well	1454:1457	well	1454:1457	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	1	127	theme	located	91:97	arg1	SLC39A8					75:81	BACKGROUND SLC39A8	64:81	BACKGROUND SLC39A8	64:81	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	1	127	theme	located	91:97	arg1	gene					86:89	a gene	84:89	a gene located on chromosome 4q24	84:116	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	7	128	with	disability	1113:1122	arg1	progression					1185:1195	a more severe progression	1171:1195	a more severe progression of the disease in the two affected siblings	1171:1239	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	2	129	theme	serum	399:403	arg1	Mn					405:406	decreased serum Mn	389:406	decreased serum Mn	389:406	The common SLC39A8 missense variant A391T is associated with increased risk for multiple neurological and systemic disorders and with decreased serum Mn.					
34246313	4	130	theme	supplementation	569:583	arg1	therapy					585:591	Galactose and Mn supplementation therapy	552:591	Galactose and Mn supplementation therapy	552:591	Galactose and Mn supplementation therapy results in the biochemical and clinical amelioration of treated patients.					
34246313	10	131	theme	isoforms	1574:1581	arg1	increase					1542:1549	slight increase	1535:1549	slight increase of trisialotransferrin isoforms or even normal pattern	1535:1604	Serum transferrin isoelectric focusing (IEF) yielded variable results with slight increase of trisialotransferrin isoforms or even normal pattern.					
34246313	7	132	theme	disease	1204:1210	arg1	progression					1185:1195	a more severe progression	1171:1195	a more severe progression of the disease in the two affected siblings	1171:1239	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	9	133	theme	consistent	1409:1418	arg1	changes					1401:1407	atrophic cerebral and cerebellum changes	1368:1407	atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG	1368:1435	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	9	133	theme	consistent	1409:1418	arg1	well					1454:1457	well	1454:1457	well	1454:1457	In the two siblings, atrophic cerebral and cerebellum changes consistent with SLC39A8-CDG were detected as well.					
34246313	1	134	theme	detrimental	175:185	arg1	variants					187:194	its detrimental variants	171:194	its detrimental variants	171:194	BACKGROUND SLC39A8, a gene located on chromosome 4q24, encodes for the manganese (Mn) transporter ZIP8 and its detrimental variants cause a type 2 congenital disorder of glycosylation (CDG).					
34246313	14	135	theme	novel	2132:2136	arg1	variants					2138:2145	two novel variants	2128:2145	two novel variants associated with Leigh syndrome-like disease presentation of SLC39A8-CDG	2128:2217	We document two novel variants associated with Leigh syndrome-like disease presentation of SLC39A8-CDG.					
34246313	15	136	theme	neurological	2258:2269	arg1	phenotype					2271:2279	a severe neurological phenotype	2249:2279	a severe neurological phenotype overlapping with that described for SLC39A8-CDG in association with the homozygous A391T missense variant	2249:2385	We show, for the first time, a severe neurological phenotype overlapping with that described for SLC39A8-CDG in association with the homozygous A391T missense variant.					
34246313	12	137	theme	sialylated	1922:1931	arg1	glycans					1945:1951	fully sialylated biantennary glycans	1916:1951	fully sialylated biantennary glycans	1916:1951	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	7	138	with	pattern	1143:1149	arg1	progression					1185:1195	a more severe progression	1171:1195	a more severe progression of the disease in the two affected siblings	1171:1239	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	17	139	theme	Further	2617:2623	arg1	studies					2625:2631	Further studies	2617:2631	Further studies	2617:2631	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	10	140	theme	pattern	1598:1604	arg1	increase					1542:1549	slight increase	1535:1549	slight increase of trisialotransferrin isoforms or even normal pattern	1535:1604	Serum transferrin isoelectric focusing (IEF) yielded variable results with slight increase of trisialotransferrin isoforms or even normal pattern.					
34246313	3	141	theme	neuroradiological	471:487	arg1	features					489:496	different clinical and neuroradiological features	448:496	different clinical and neuroradiological features linked to variable transferrin glycosylation profile	448:549	Patients with SLC39A8-CDG present with different clinical and neuroradiological features linked to variable transferrin glycosylation profile.					
34246313	12	142	theme	species	2002:2008	arg1	A2G1S1					2010:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	12	143	theme	glycans	1945:1951	arg1	precursors					1902:1911	undergalactosylated and undersialylated precursors	1862:1911	undergalactosylated and undersialylated precursors	1862:1911	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	12	143	theme	glycans	1945:1951	arg1	A2G1S1					2010:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	7	144	with	dyskinesia	1155:1164	arg1	progression					1185:1195	a more severe progression	1171:1195	a more severe progression of the disease in the two affected siblings	1171:1239	The clinical phenotype of the three patients was characterized by severe developmental disability, dystonic postural pattern and dyskinesia with a more severe progression of the disease in the two affected siblings.					
34246313	10	145	theme	even	1586:1589	arg1	pattern					1598:1604	even normal pattern	1586:1604	even normal pattern	1586:1604	Serum transferrin isoelectric focusing (IEF) yielded variable results with slight increase of trisialotransferrin isoforms or even normal pattern.					
34246313	3	146	theme	clinical	458:465	arg1	features					489:496	different clinical and neuroradiological features	448:496	different clinical and neuroradiological features linked to variable transferrin glycosylation profile	448:549	Patients with SLC39A8-CDG present with different clinical and neuroradiological features linked to variable transferrin glycosylation profile.					
34246313	8	147	theme	white	1333:1337	arg1	matter					1339:1344	white matter	1333:1344	white matter	1333:1344	Neuroimaging showed a Leigh syndrome-like pattern involving the basal ganglia, thalami and white matter.					
34246313	12	148	theme	undersialylated	1886:1900	arg1	precursors					1902:1911	undergalactosylated and undersialylated precursors	1862:1911	undergalactosylated and undersialylated precursors	1862:1911	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	12	148	theme	undersialylated	1886:1900	arg1	A2G1S1					2010:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	the monosialo-monogalacto-biantennary species A2G1S1	1964:2015	Total serum N-glycome depicted a distinct pattern for the three patients, with increased levels of undergalactosylated and undersialylated precursors of fully sialylated biantennary glycans, including the monosialo-monogalacto-biantennary species A2G1S1.					
34246313	17	149	theme	variants	2694:2701	arg1	influence					2673:2681	the influence	2669:2681	the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features	2669:2862	Further studies are needed to analyse more in depth the influence of SLC39A8 variants, including the common missense variant, on the expression and function of ZIP8 protein, and their impact on clinical, biochemical and neuroradiological features.					
34246313	3	150	theme	variable	508:515	arg1	profile					543:549	variable transferrin glycosylation profile	508:549	variable transferrin glycosylation profile	508:549	Patients with SLC39A8-CDG present with different clinical and neuroradiological features linked to variable transferrin glycosylation profile.					
32475543	4	0	theme	thermal	873:879	arg1	group					891:895	the solely thermal treatment group	862:895	the solely thermal treatment group	862:895	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	1	theme	CSF	784:786	arg1	CSF					784:786	CSF	784:786	CSF	784:786	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	1	theme	CSF	784:786	arg1	amount					774:779	the total amount	764:779	the total amount of CSF	764:786	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	2	theme	pectin	589:594	arg1	monosaccharides					615:629	monosaccharides composition, esterification degree and sugar ratios	615:680	monosaccharides composition, esterification degree and sugar ratios	615:680	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	2	theme	pectin	589:594	arg1	contents					605:612	The pectin fraction contents	585:612	The pectin fraction contents	585:612	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	6	3	theme	plant-based	1151:1161	arg1	texture					1185:1191	the plant-based vegetables processing texture	1147:1191	the plant-based vegetables processing texture in food industry	1147:1208	This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.					
32475543	0	4	theme	chelate-soluble	85:99	arg1	polysaccharides					101:115	the chelate-soluble polysaccharides	81:115	the chelate-soluble polysaccharides	81:115	Acetic acid reducing the softening of lotus rhizome during heating by regulating the chelate-soluble polysaccharides.					
32475543	5	5	theme	backbone	1012:1019	arg1	structure					995:1003	helical structure	987:1003	helical structure of CSF backbone	987:1019	The nanostructures showed that acetic acid treatment maintained longer chain and destroy helical structure of CSF backbone.					
32475543	6	6	dep	polysaccharides	1081:1095	arg1	structure					1097:1105	structure	1097:1105	structure	1097:1105	This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.					
32475543	6	6	dep	polysaccharides	1081:1095	arg1	texture					1118:1124	cooked texture	1111:1124	cooked texture	1111:1124	This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.					
32475543	6	6	dep	polysaccharides	1081:1095	arg1	polysaccharides					1081:1095	polysaccharides structure and cooked texture	1081:1124	polysaccharides structure and cooked texture	1081:1124	This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.					
32475543	3	7	theme	carbonate-soluble	530:546	arg1	fraction					548:555	sodium carbonate-soluble fraction	523:555	sodium carbonate-soluble fraction	523:555	Then, the cell walls were sequentially extracted and divided into water-soluble fraction, chelate-soluble fraction (CSF), sodium carbonate-soluble fraction and hemicellulose fraction.					
32475543	6	8	theme	processing	1174:1183	arg1	texture					1185:1191	the plant-based vegetables processing texture	1147:1191	the plant-based vegetables processing texture in food industry	1147:1208	This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.					
32475543	1	9	used	used	138:141	arg2	rhizomes					124:131	Lotus rhizomes	118:131	Lotus rhizomes	118:131	Lotus rhizomes were used to study on the relationship between the cell wall polysaccharides and cooked texture by adding acetic acid.					
32475543	4	10	theme	treatment	881:889	arg1	group					891:895	the solely thermal treatment group	862:895	the solely thermal treatment group	862:895	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	2	11	theme	microscopy	283:292	arg1	results					294:300	Hardness and scanning electron microscopy results	252:300	Hardness and scanning electron microscopy results	252:300	Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall.					
32475543	4	12	theme	fraction	596:603	arg1	monosaccharides					615:629	monosaccharides composition, esterification degree and sugar ratios	615:680	monosaccharides composition, esterification degree and sugar ratios	615:680	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	12	theme	fraction	596:603	arg1	contents					605:612	The pectin fraction contents	585:612	The pectin fraction contents	585:612	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	2	13	theme	higher	349:354	arg1	hardness					356:363	higher hardness	349:363	higher hardness	349:363	Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall.					
32475543	2	14	theme	electron	274:281	arg1	microscopy					283:292	scanning electron microscopy	265:292	scanning electron microscopy	265:292	Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall.					
32475543	5	15	theme	acid	936:939	arg1	treatment					941:949	acetic acid treatment	929:949	acetic acid treatment	929:949	The nanostructures showed that acetic acid treatment maintained longer chain and destroy helical structure of CSF backbone.					
32475543	2	16	theme	wall	395:398	arg1	hardness					356:363	higher hardness	349:363	higher hardness	349:363	Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall.					
32475543	2	16	theme	wall	395:398	arg1	integrity					373:381	the integrity	369:381	the integrity of the cell wall	369:398	Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall.					
32475543	2	17	theme	scanning	265:272	arg1	microscopy					283:292	scanning electron microscopy	265:292	scanning electron microscopy	265:292	Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall.					
32475543	2	18	theme	cell	390:393	arg1	wall					395:398	the cell wall	386:398	the cell wall	386:398	Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall.					
32475543	6	19	theme	food	1196:1199	arg1	industry					1201:1208	food industry	1196:1208	food industry	1196:1208	This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.					
32475543	2	20	theme	acid	321:324	arg1	treatment					326:334	acetic acid treatment	314:334	acetic acid treatment	314:334	Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall.					
32475543	3	21	theme	cell	411:414	arg1	walls					416:420	the cell walls	407:420	the cell walls	407:420	Then, the cell walls were sequentially extracted and divided into water-soluble fraction, chelate-soluble fraction (CSF), sodium carbonate-soluble fraction and hemicellulose fraction.					
32475543	0	22	theme	Acetic	0:5	arg1	acid					7:10	Acetic acid	0:10	Acetic acid	0:10	Acetic acid reducing the softening of lotus rhizome during heating by regulating the chelate-soluble polysaccharides.					
32475543	4	23	dep	increased	754:762	arg1	decreased					789:797	decreased	789:797	decreased the esterification degree and less side chain compared that in the solely thermal treatment group	789:895	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	2	24	theme	acetic	314:319	arg1	treatment					326:334	acetic acid treatment	314:334	acetic acid treatment	314:334	Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall.					
32475543	6	25	from	texture	1185:1191	arg1	industry					1201:1208	food industry	1196:1208	food industry	1196:1208	This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.					
32475543	1	26	theme	cell	184:187	arg1	polysaccharides					194:208	the cell wall polysaccharides	180:208	the cell wall polysaccharides	180:208	Lotus rhizomes were used to study on the relationship between the cell wall polysaccharides and cooked texture by adding acetic acid.					
32475543	4	27	theme	total	768:772	arg1	CSF					784:786	CSF	784:786	CSF	784:786	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	27	theme	total	768:772	arg1	amount					774:779	the total amount	764:779	the total amount of CSF	764:786	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	1	28	theme	wall	189:192	arg1	polysaccharides					194:208	the cell wall polysaccharides	180:208	the cell wall polysaccharides	180:208	Lotus rhizomes were used to study on the relationship between the cell wall polysaccharides and cooked texture by adding acetic acid.					
32475543	5	29	theme	longer	962:967	arg1	chain					969:973	longer chain	962:973	longer chain	962:973	The nanostructures showed that acetic acid treatment maintained longer chain and destroy helical structure of CSF backbone.					
32475543	3	30	theme	hemicellulose	561:573	arg1	fraction					575:582	hemicellulose fraction	561:582	hemicellulose fraction	561:582	Then, the cell walls were sequentially extracted and divided into water-soluble fraction, chelate-soluble fraction (CSF), sodium carbonate-soluble fraction and hemicellulose fraction.					
32475543	4	31	theme	esterification	644:656	arg1	monosaccharides					615:629	monosaccharides composition, esterification degree and sugar ratios	615:680	monosaccharides composition, esterification degree and sugar ratios	615:680	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	31	theme	esterification	644:656	arg1	degree					658:663	esterification degree	644:663	esterification degree	644:663	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	5	32	theme	acetic	929:934	arg1	treatment					941:949	acetic acid treatment	929:949	acetic acid treatment	929:949	The nanostructures showed that acetic acid treatment maintained longer chain and destroy helical structure of CSF backbone.					
32475543	4	33	theme	different	685:693	arg1	groups					695:700	different groups	685:700	different groups	685:700	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	34	theme	sugar	669:673	arg1	ratios					675:680	sugar ratios	669:680	sugar ratios	669:680	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	6	35	theme	cooked	1111:1116	arg1	texture					1118:1124	cooked texture	1111:1124	cooked texture	1111:1124	This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.					
32475543	6	35	theme	cooked	1111:1116	arg1	polysaccharides					1081:1095	polysaccharides structure and cooked texture	1081:1124	polysaccharides structure and cooked texture	1081:1124	This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.					
32475543	1	36	theme	cooked	214:219	arg1	texture					221:227	cooked texture	214:227	cooked texture	214:227	Lotus rhizomes were used to study on the relationship between the cell wall polysaccharides and cooked texture by adding acetic acid.					
32475543	0	37	theme	rhizome	44:50	arg1	softening					25:33	the softening	21:33	the softening of lotus rhizome during heating	21:65	Acetic acid reducing the softening of lotus rhizome during heating by regulating the chelate-soluble polysaccharides.					
32475543	4	38	from	contents	605:612	arg1	groups					695:700	different groups	685:700	different groups	685:700	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	5	39	theme	helical	987:993	arg1	structure					995:1003	helical structure	987:1003	helical structure of CSF backbone	987:1019	The nanostructures showed that acetic acid treatment maintained longer chain and destroy helical structure of CSF backbone.					
32475543	1	40	theme	Lotus	118:122	arg1	rhizomes					124:131	Lotus rhizomes	118:131	Lotus rhizomes	118:131	Lotus rhizomes were used to study on the relationship between the cell wall polysaccharides and cooked texture by adding acetic acid.					
32475543	0	41	theme	lotus	38:42	arg1	rhizome					44:50	lotus rhizome	38:50	lotus rhizome	38:50	Acetic acid reducing the softening of lotus rhizome during heating by regulating the chelate-soluble polysaccharides.					
32475543	4	42	theme	side	834:837	arg1	chain					839:843	less side chain	829:843	less side chain	829:843	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	3	43	theme	chelate-soluble	491:505	arg1	CSF					517:519	CSF	517:519	CSF	517:519	Then, the cell walls were sequentially extracted and divided into water-soluble fraction, chelate-soluble fraction (CSF), sodium carbonate-soluble fraction and hemicellulose fraction.					
32475543	3	43	theme	chelate-soluble	491:505	arg1	fraction					507:514	chelate-soluble fraction	491:514	chelate-soluble fraction (CSF)	491:520	Then, the cell walls were sequentially extracted and divided into water-soluble fraction, chelate-soluble fraction (CSF), sodium carbonate-soluble fraction and hemicellulose fraction.					
32475543	5	44	theme	CSF	1008:1010	arg1	backbone					1012:1019	CSF backbone	1008:1019	CSF backbone	1008:1019	The nanostructures showed that acetic acid treatment maintained longer chain and destroy helical structure of CSF backbone.					
32475543	3	45	theme	water-soluble	467:479	arg1	fraction					481:488	water-soluble fraction	467:488	water-soluble fraction	467:488	Then, the cell walls were sequentially extracted and divided into water-soluble fraction, chelate-soluble fraction (CSF), sodium carbonate-soluble fraction and hemicellulose fraction.					
32475543	3	46	theme	sodium	523:528	arg1	fraction					548:555	sodium carbonate-soluble fraction	523:555	sodium carbonate-soluble fraction	523:555	Then, the cell walls were sequentially extracted and divided into water-soluble fraction, chelate-soluble fraction (CSF), sodium carbonate-soluble fraction and hemicellulose fraction.					
32475543	4	47	dep	monosaccharides	615:629	arg1	monosaccharides					615:629	monosaccharides composition, esterification degree and sugar ratios	615:680	monosaccharides composition, esterification degree and sugar ratios	615:680	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	47	dep	monosaccharides	615:629	arg1	composition					631:641	composition	631:641	composition	631:641	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	47	dep	monosaccharides	615:629	arg1	degree					658:663	esterification degree	644:663	esterification degree	644:663	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	47	dep	monosaccharides	615:629	arg1	ratios					675:680	sugar ratios	669:680	sugar ratios	669:680	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	2	48	theme	Hardness	252:259	arg1	results					294:300	Hardness and scanning electron microscopy results	252:300	Hardness and scanning electron microscopy results	252:300	Hardness and scanning electron microscopy results showed that acetic acid treatment can maintain higher hardness and the integrity of the cell wall.					
32475543	6	49	theme	vegetables	1163:1172	arg1	texture					1185:1191	the plant-based vegetables processing texture	1147:1191	the plant-based vegetables processing texture in food industry	1147:1208	This work helps us to demonstrate the relationship between polysaccharides structure and cooked texture, and further control the plant-based vegetables processing texture in food industry.					
32475543	4	50	theme	acetic	742:747	arg1	acid					749:752	acetic acid	742:752	acetic acid	742:752	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	4	51	dep	esterification	803:816	arg1	compared					845:852	compared	845:852	compared that in the solely thermal treatment group	845:895	The pectin fraction contents, monosaccharides composition, esterification degree and sugar ratios in different groups were evaluated, the results showed that acetic acid increased the total amount of CSF, decreased the esterification degree and less side chain compared that in the solely thermal treatment group.					
32475543	1	52	theme	acetic	239:244	arg1	acid					246:249	acetic acid	239:249	acetic acid	239:249	Lotus rhizomes were used to study on the relationship between the cell wall polysaccharides and cooked texture by adding acetic acid.					
32289476	11	0	theme	neutral	1527:1533	arg1	heteropolysaccharide					1535:1554	a neutral heteropolysaccharide	1525:1554	a neutral heteropolysaccharide with molecular weight	1525:1576	RESULTS Physicochemical and structural analyses showed that SB2-1 was a neutral heteropolysaccharide with molecular weight at 22.2 kDa and consisted of glucose, galactose and mannose at a ratio of 2.0:1.5:1.0.					
32289476	11	0	theme	neutral	1527:1533	arg1	SB2-1					1515:1519	SB2-1	1515:1519	SB2-1	1515:1519	RESULTS Physicochemical and structural analyses showed that SB2-1 was a neutral heteropolysaccharide with molecular weight at 22.2 kDa and consisted of glucose, galactose and mannose at a ratio of 2.0:1.5:1.0.					
32289476	5	1	theme	Shiraia	661:667	arg1	bambusicola					669:679	Shiraia bambusicola	661:679	Shiraia bambusicola	661:679	To explore bioactive polysaccharides from Shiraia bambusicola, a heteropolysaccharide SB2-1 was purified and obtained from S. bambusicola and its immunostimulating activity was researched.					
32289476	2	2	theme	stomach	367:373	arg1	pain					375:378	cold stomach pain	362:378	cold stomach pain	362:378	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	14	3	dep	-Manp	1994:1998	arg1	1→2					1990:1992	1→2	1990:1992	1→2	1990:1992	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	16	4	theme	SB2-1	2186:2190	arg1	activities					2172:2181	the immunostimulating activities	2150:2181	the immunostimulating activities of SB2-1 and its core structure P-2	2150:2217	The study on the immunostimulating activities of SB2-1 and its core structure P-2 were investigated on RAW264.7 macrophages.					
32289476	6	5	theme	ethanol	930:936	arg1	precipitation					938:950	ethanol precipitation	930:950	ethanol precipitation	930:950	MATERIALS AND METHODS The polysaccharide from S. bambusicola was extracted and purified using enzyme assisted extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.					
32289476	9	6	theme	gradual	1329:1335	arg1	spectrum					1282:1289	spectrum	1282:1289	spectrum	1282:1289	Structure information was elucidated by IR spectrum, GC-MS analysis after methylation and gradual acid hydrolysis of the polysaccharide.					
32289476	9	6	theme	gradual	1329:1335	arg1	hydrolysis					1342:1351	gradual acid hydrolysis	1329:1351	gradual acid hydrolysis of the polysaccharide	1329:1373	Structure information was elucidated by IR spectrum, GC-MS analysis after methylation and gradual acid hydrolysis of the polysaccharide.					
32289476	19	7	theme	immunostimulating	2716:2732	arg1	activities					2734:2743	the immunostimulating activities	2712:2743	the immunostimulating activities	2712:2743	The effect of SB2-1 was better than its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities.					
32289476	12	8	theme	side	1749:1752	arg1	chains					1754:1759	side chains	1749:1759	side chains	1749:1759	The structure of SB2-1 was a branched polysaccharides composed of a mannan core and side chains consisted of glucose and galactose.					
32289476	20	9	theme	galactofuranose-containing	2798:2823	arg1	mannogalactoglucan					2825:2842	the galactofuranose-containing mannogalactoglucan	2794:2842	the galactofuranose-containing mannogalactoglucan	2794:2842	CONCLUSIONS The investigation demonstrated that the galactofuranose-containing mannogalactoglucan was characteristic polysaccharides in S. bambusicola and could enhance the activation of macrophages.					
32289476	20	9	theme	galactofuranose-containing	2798:2823	arg1	polysaccharides					2863:2877	characteristic polysaccharides	2848:2877	characteristic polysaccharides	2848:2877	CONCLUSIONS The investigation demonstrated that the galactofuranose-containing mannogalactoglucan was characteristic polysaccharides in S. bambusicola and could enhance the activation of macrophages.					
32289476	1	10	theme	bamboos	234:240	arg1	twigs					225:229	the twigs	221:229	the twigs of bamboos	221:240	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola is a parasitic fungus on the twigs of bamboos.					
32289476	14	11	dep	-D-Glcp	1891:1897	arg1	1→2					1887:1889	1→2	1887:1889	1→2	1887:1889	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	17	12	theme	phagocytic	2357:2366	arg1	ability					2368:2374	its phagocytic ability	2353:2374	its phagocytic ability	2353:2374	The results showed that SB2-1 could activate RAW264.7 macrophage and significantly improve its phagocytic ability by neutral red uptake experiment.					
32289476	3	13	located	found	514:518	arg1	Japan					523:527	Japan	523:527	Japan	523:527	It is widely distributed in many provinces in Southern China and also is also found in Japan.					
32289476	3	13	located	found	514:518	arg2	It					436:437	It	436:437	It	436:437	It is widely distributed in many provinces in Southern China and also is also found in Japan.					
32289476	5	14	theme	heteropolysaccharide	684:703	arg1	SB2-1					705:709	a heteropolysaccharide SB2-1	682:709	a heteropolysaccharide SB2-1	682:709	To explore bioactive polysaccharides from Shiraia bambusicola, a heteropolysaccharide SB2-1 was purified and obtained from S. bambusicola and its immunostimulating activity was researched.					
32289476	7	15	theme	permeation	1076:1085	arg1	chromatography					1087:1100	high performance gel permeation chromatography	1055:1100	high performance gel permeation chromatography	1055:1100	Molecular weight of polysaccharide was estimated by high performance gel permeation chromatography.					
32289476	1	16	theme	ETHNOPHARMACOLOGICAL	145:164	arg1	RELEVANCE					166:174	ETHNOPHARMACOLOGICAL RELEVANCE	145:174	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola	145:194	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola is a parasitic fungus on the twigs of bamboos.					
32289476	1	16	theme	ETHNOPHARMACOLOGICAL	145:164	arg1	fungus					211:216	a parasitic fungus	199:216	a parasitic fungus on the twigs of bamboos	199:240	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola is a parasitic fungus on the twigs of bamboos.					
32289476	7	17	theme	performance	1060:1070	arg1	chromatography					1087:1100	high performance gel permeation chromatography	1055:1100	high performance gel permeation chromatography	1055:1100	Molecular weight of polysaccharide was estimated by high performance gel permeation chromatography.					
32289476	14	18	theme	different	1919:1927	arg1	degrees					1929:1935	different degrees	1919:1935	different degrees of polymerization	1919:1953	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	12	19	theme	branched	1694:1701	arg1	polysaccharides					1703:1717	a branched polysaccharides	1692:1717	a branched polysaccharides composed of a mannan core and side chains consisted of glucose and galactose	1692:1794	The structure of SB2-1 was a branched polysaccharides composed of a mannan core and side chains consisted of glucose and galactose.					
32289476	12	19	theme	branched	1694:1701	arg1	structure					1669:1677	The structure	1665:1677	The structure of SB2-1	1665:1686	The structure of SB2-1 was a branched polysaccharides composed of a mannan core and side chains consisted of glucose and galactose.					
32289476	17	20	theme	RAW264.7	2307:2314	arg1	macrophage					2316:2325	RAW264.7 macrophage	2307:2325	RAW264.7 macrophage	2307:2325	The results showed that SB2-1 could activate RAW264.7 macrophage and significantly improve its phagocytic ability by neutral red uptake experiment.					
32289476	19	21	theme	SB2-1	2574:2578	arg1	effect					2564:2569	The effect	2560:2569	The effect of SB2-1	2560:2578	The effect of SB2-1 was better than its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities.					
32289476	19	21	theme	SB2-1	2574:2578	arg1	better					2584:2589	better	2584:2589	better	2584:2589	The effect of SB2-1 was better than its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities.					
32289476	2	22	theme	high	275:278	arg1	value					290:294	high medicinal value	275:294	high medicinal value	275:294	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	1	23	from	fungus	211:216	arg1	twigs					225:229	the twigs	221:229	the twigs of bamboos	221:240	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola is a parasitic fungus on the twigs of bamboos.					
32289476	17	24	theme	uptake	2391:2396	arg1	experiment					2398:2407	neutral red uptake experiment	2379:2407	neutral red uptake experiment	2379:2407	The results showed that SB2-1 could activate RAW264.7 macrophage and significantly improve its phagocytic ability by neutral red uptake experiment.					
32289476	0	25	theme	bamboo	91:96	arg1	bambusicola					132:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	1	26	theme	parasitic	201:209	arg1	RELEVANCE					166:174	ETHNOPHARMACOLOGICAL RELEVANCE	145:174	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola	145:194	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola is a parasitic fungus on the twigs of bamboos.					
32289476	1	26	theme	parasitic	201:209	arg1	fungus					211:216	a parasitic fungus	199:216	a parasitic fungus on the twigs of bamboos	199:240	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola is a parasitic fungus on the twigs of bamboos.					
32289476	0	27	from	Structure	0:8	arg1	bambusicola					132:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	0	28	theme	medicinal	107:115	arg1	bambusicola					132:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	1	29	theme	Shiraia	176:182	arg1	RELEVANCE					166:174	ETHNOPHARMACOLOGICAL RELEVANCE	145:174	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola	145:194	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola is a parasitic fungus on the twigs of bamboos.					
32289476	1	29	theme	Shiraia	176:182	arg1	fungus					211:216	a parasitic fungus	199:216	a parasitic fungus on the twigs of bamboos	199:240	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola is a parasitic fungus on the twigs of bamboos.					
32289476	18	30	theme	IL-1	2531:2534	arg1	production					2497:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	18	30	theme	IL-1	2531:2534	arg1	productions					2516:2526	the productions	2512:2526	the productions of IL-1, IL-6, IL-12 and TNF-α	2512:2557	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	10	31	theme	immunostimulating	1418:1434	arg1	activity					1436:1443	the immunostimulating activity	1414:1443	the immunostimulating activity	1414:1443	The RAW264.7 cells were used to study the immunostimulating activity in vitro.					
32289476	2	32	theme	chronic	401:407	arg1	bronchitis					409:418	chronic bronchitis	401:418	chronic bronchitis	401:418	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	0	33	theme	Shiraia	124:130	arg1	bambusicola					132:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	8	34	theme	high	1150:1153	arg1	chromatography					1174:1187	high performance liquid chromatography	1150:1187	high performance liquid chromatography	1150:1187	Monosaccharide compositions were determined by high performance liquid chromatography after pre-column derivatization and UV detection.					
32289476	7	35	theme	Molecular	1003:1011	arg1	weight					1013:1018	Molecular weight	1003:1018	Molecular weight of polysaccharide	1003:1036	Molecular weight of polysaccharide was estimated by high performance gel permeation chromatography.					
32289476	16	36	theme	core	2200:2203	arg1	P-2					2215:2217	its core structure P-2	2196:2217	its core structure P-2	2196:2217	The study on the immunostimulating activities of SB2-1 and its core structure P-2 were investigated on RAW264.7 macrophages.					
32289476	8	37	theme	liquid	1167:1172	arg1	chromatography					1174:1187	high performance liquid chromatography	1150:1187	high performance liquid chromatography	1150:1187	Monosaccharide compositions were determined by high performance liquid chromatography after pre-column derivatization and UV detection.					
32289476	17	38	theme	red	2387:2389	arg1	experiment					2398:2407	neutral red uptake experiment	2379:2407	neutral red uptake experiment	2379:2407	The results showed that SB2-1 could activate RAW264.7 macrophage and significantly improve its phagocytic ability by neutral red uptake experiment.					
32289476	18	39	theme	inducible	2458:2466	arg1	iNOS					2491:2494	iNOS	2491:2494	iNOS	2491:2494	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	18	39	theme	inducible	2458:2466	arg1	synthase					2481:2488	inducible nitric oxide synthase	2458:2488	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	3	40	theme	many	464:467	arg1	provinces					469:477	many provinces	464:477	many provinces in Southern China	464:495	It is widely distributed in many provinces in Southern China and also is also found in Japan.					
32289476	0	41	theme	immunostimulating	14:30	arg1	activity					32:39	immunostimulating activity	14:39	immunostimulating activity	14:39	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	6	42	theme	assisted	909:916	arg1	extraction					918:927	enzyme assisted extraction	902:927	enzyme assisted extraction	902:927	MATERIALS AND METHODS The polysaccharide from S. bambusicola was extracted and purified using enzyme assisted extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.					
32289476	19	43	theme	acid	2639:2642	arg1	hydrolysis					2644:2653	gradual acid hydrolysis	2631:2653	gradual acid hydrolysis	2631:2653	The effect of SB2-1 was better than its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities.					
32289476	4	44	theme	STUDY	541:545	arg1	fungi					557:561	THE STUDY Medicinal fungi	537:561	THE STUDY Medicinal fungi	537:561	AIM OF THE STUDY Medicinal fungi were important resources for bioactive polysaccharides.					
32289476	9	45	theme	Structure	1239:1247	arg1	information					1249:1259	Structure information	1239:1259	Structure information	1239:1259	Structure information was elucidated by IR spectrum, GC-MS analysis after methylation and gradual acid hydrolysis of the polysaccharide.					
32289476	5	46	from	bambusicola	669:679	arg1	polysaccharides					640:654	bioactive polysaccharides	630:654	bioactive polysaccharides from Shiraia bambusicola	630:679	To explore bioactive polysaccharides from Shiraia bambusicola, a heteropolysaccharide SB2-1 was purified and obtained from S. bambusicola and its immunostimulating activity was researched.					
32289476	14	47	theme	side	2007:2010	arg1	chains					2012:2017	the side chains	2003:2017	the side chains	2003:2017	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	2	48	theme	cold	362:365	arg1	pain					375:378	cold stomach pain	362:378	cold stomach pain	362:378	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	0	49	theme	galactofuranose-rich	46:65	arg1	polysaccharide					67:80	a galactofuranose-rich polysaccharide	44:80	a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola	44:142	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	8	50	theme	UV	1225:1226	arg1	detection					1228:1236	UV detection	1225:1236	UV detection	1225:1236	Monosaccharide compositions were determined by high performance liquid chromatography after pre-column derivatization and UV detection.					
32289476	15	51	theme	1→	2102:2103	arg1	linages					2043:2049	the linages	2039:2049	the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→	2039:2103	The galactose with the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→ also existed in the side chain.					
32289476	18	52	theme	oxide	2475:2479	arg1	iNOS					2491:2494	iNOS	2491:2494	iNOS	2491:2494	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	18	52	theme	oxide	2475:2479	arg1	synthase					2481:2488	inducible nitric oxide synthase	2458:2488	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	3	53	from	provinces	469:477	arg1	China					491:495	Southern China	482:495	Southern China	482:495	It is widely distributed in many provinces in Southern China and also is also found in Japan.					
32289476	15	54	theme	D-Galf	2095:2100	arg1	1→					2102:2103	terminal D-Galf(1→	2086:2103	terminal D-Galf(1→	2086:2103	The galactose with the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→ also existed in the side chain.					
32289476	16	55	theme	immunostimulating	2154:2170	arg1	activities					2172:2181	the immunostimulating activities	2150:2181	the immunostimulating activities of SB2-1 and its core structure P-2	2150:2217	The study on the immunostimulating activities of SB2-1 and its core structure P-2 were investigated on RAW264.7 macrophages.					
32289476	11	56	with	heteropolysaccharide	1535:1554	arg1	weight					1571:1576	molecular weight	1561:1576	molecular weight	1561:1576	RESULTS Physicochemical and structural analyses showed that SB2-1 was a neutral heteropolysaccharide with molecular weight at 22.2 kDa and consisted of glucose, galactose and mannose at a ratio of 2.0:1.5:1.0.					
32289476	6	57	theme	size-exclusion	972:985	arg1	chromatography					987:1000	size-exclusion chromatography	972:1000	size-exclusion chromatography	972:1000	MATERIALS AND METHODS The polysaccharide from S. bambusicola was extracted and purified using enzyme assisted extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.					
32289476	18	58	theme	IL-12	2543:2547	arg1	production					2497:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	18	58	theme	IL-12	2543:2547	arg1	productions					2516:2526	the productions	2512:2526	the productions of IL-1, IL-6, IL-12 and TNF-α	2512:2557	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	14	59	attach	linked	1960:1965	arg2	Glucose					1859:1865	Glucose	1859:1865	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization	1859:1953	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	14	59	attach	linked	1960:1965	arg3	C-6					1970:1972	C-6	1970:1972	C-6	1970:1972	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	14	59	attach	linked	1960:1965	arg3	C-3					1978:1980	C-3	1978:1980	C-3	1978:1980	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	9	60	theme	GC-MS	1292:1296	arg1	spectrum					1282:1289	spectrum	1282:1289	spectrum	1282:1289	Structure information was elucidated by IR spectrum, GC-MS analysis after methylation and gradual acid hydrolysis of the polysaccharide.					
32289476	9	60	theme	GC-MS	1292:1296	arg1	analysis					1298:1305	GC-MS analysis	1292:1305	GC-MS analysis after methylation	1292:1323	Structure information was elucidated by IR spectrum, GC-MS analysis after methylation and gradual acid hydrolysis of the polysaccharide.					
32289476	19	61	theme	side	2672:2675	arg1	chains					2677:2682	the side chains	2668:2682	the side chains played an important role in the immunostimulating activities	2668:2743	The effect of SB2-1 was better than its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities.					
32289476	4	62	theme	fungi	557:561	arg1	AIM					530:532	AIM	530:532	AIM OF THE STUDY Medicinal fungi	530:561	AIM OF THE STUDY Medicinal fungi were important resources for bioactive polysaccharides.					
32289476	4	63	theme	important	568:576	arg1	resources					578:586	important resources	568:586	important resources for bioactive polysaccharides	568:616	AIM OF THE STUDY Medicinal fungi were important resources for bioactive polysaccharides.					
32289476	11	64	theme	molecular	1561:1569	arg1	weight					1571:1576	molecular weight	1561:1576	molecular weight	1561:1576	RESULTS Physicochemical and structural analyses showed that SB2-1 was a neutral heteropolysaccharide with molecular weight at 22.2 kDa and consisted of glucose, galactose and mannose at a ratio of 2.0:1.5:1.0.					
32289476	11	65	theme	structural	1483:1492	arg1	analyses					1494:1501	structural analyses	1483:1501	structural analyses	1483:1501	RESULTS Physicochemical and structural analyses showed that SB2-1 was a neutral heteropolysaccharide with molecular weight at 22.2 kDa and consisted of glucose, galactose and mannose at a ratio of 2.0:1.5:1.0.					
32289476	5	66	theme	bioactive	630:638	arg1	polysaccharides					640:654	bioactive polysaccharides	630:654	bioactive polysaccharides from Shiraia bambusicola	630:679	To explore bioactive polysaccharides from Shiraia bambusicola, a heteropolysaccharide SB2-1 was purified and obtained from S. bambusicola and its immunostimulating activity was researched.					
32289476	9	67	theme	acid	1337:1340	arg1	spectrum					1282:1289	spectrum	1282:1289	spectrum	1282:1289	Structure information was elucidated by IR spectrum, GC-MS analysis after methylation and gradual acid hydrolysis of the polysaccharide.					
32289476	9	67	theme	acid	1337:1340	arg1	hydrolysis					1342:1351	gradual acid hydrolysis	1329:1351	gradual acid hydrolysis of the polysaccharide	1329:1373	Structure information was elucidated by IR spectrum, GC-MS analysis after methylation and gradual acid hydrolysis of the polysaccharide.					
32289476	15	68	with	galactose	2024:2032	arg1	linages					2043:2049	the linages	2039:2049	the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→	2039:2103	The galactose with the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→ also existed in the side chain.					
32289476	8	69	theme	Monosaccharide	1103:1116	arg1	compositions					1118:1129	Monosaccharide compositions	1103:1129	Monosaccharide compositions	1103:1129	Monosaccharide compositions were determined by high performance liquid chromatography after pre-column derivatization and UV detection.					
32289476	6	70	dep	polysaccharide	834:847	arg1	bambusicola					857:867	S. bambusicola	854:867	S. bambusicola	854:867	MATERIALS AND METHODS The polysaccharide from S. bambusicola was extracted and purified using enzyme assisted extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.					
32289476	18	71	theme	IL-6	2537:2540	arg1	production					2497:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	18	71	theme	IL-6	2537:2540	arg1	productions					2516:2526	the productions	2512:2526	the productions of IL-1, IL-6, IL-12 and TNF-α	2512:2557	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	14	72	dep	-D-Glcp	1877:1883	arg1	1→4					1873:1875	1→4	1873:1875	1→4	1873:1875	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	2	73	theme	diseases	323:330	arg1	diseases					323:330	diseases	323:330	diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile	323:433	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	2	73	theme	diseases	323:330	arg1	pain					375:378	cold stomach pain	362:378	cold stomach pain	362:378	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	2	73	theme	diseases	323:330	arg1	bronchitis					409:418	chronic bronchitis	401:418	chronic bronchitis	401:418	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	2	73	theme	diseases	323:330	arg1	sciatica					381:388	sciatica	381:388	sciatica	381:388	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	2	73	theme	diseases	323:330	arg1	variety					312:318	a variety	310:318	a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile	310:433	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	2	73	theme	diseases	323:330	arg1	arthritis					351:359	rheumatoid arthritis	340:359	rheumatoid arthritis	340:359	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	2	73	theme	diseases	323:330	arg1	infantile					425:433	infantile	425:433	infantile	425:433	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	2	73	theme	diseases	323:330	arg1	injuries					391:398	injuries	391:398	injuries	391:398	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	15	74	theme	side	2125:2128	arg1	chain					2130:2134	the side chain	2121:2134	the side chain	2121:2134	The galactose with the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→ also existed in the side chain.					
32289476	13	75	theme	main	1847:1850	arg1	core					1808:1811	The mannan core	1797:1811	The mannan core	1797:1811	The mannan core was composed of (1→2)-Manp as the main chain.					
32289476	13	75	theme	main	1847:1850	arg1	chain					1852:1856	the main chain	1843:1856	the main chain	1843:1856	The mannan core was composed of (1→2)-Manp as the main chain.					
32289476	20	76	theme	characteristic	2848:2861	arg1	mannogalactoglucan					2825:2842	the galactofuranose-containing mannogalactoglucan	2794:2842	the galactofuranose-containing mannogalactoglucan	2794:2842	CONCLUSIONS The investigation demonstrated that the galactofuranose-containing mannogalactoglucan was characteristic polysaccharides in S. bambusicola and could enhance the activation of macrophages.					
32289476	20	76	theme	characteristic	2848:2861	arg1	polysaccharides					2863:2877	characteristic polysaccharides	2848:2877	characteristic polysaccharides	2848:2877	CONCLUSIONS The investigation demonstrated that the galactofuranose-containing mannogalactoglucan was characteristic polysaccharides in S. bambusicola and could enhance the activation of macrophages.					
32289476	9	77	theme	polysaccharide	1360:1373	arg1	spectrum					1282:1289	spectrum	1282:1289	spectrum	1282:1289	Structure information was elucidated by IR spectrum, GC-MS analysis after methylation and gradual acid hydrolysis of the polysaccharide.					
32289476	9	77	theme	polysaccharide	1360:1373	arg1	analysis					1298:1305	GC-MS analysis	1292:1305	GC-MS analysis after methylation	1292:1323	Structure information was elucidated by IR spectrum, GC-MS analysis after methylation and gradual acid hydrolysis of the polysaccharide.					
32289476	9	77	theme	polysaccharide	1360:1373	arg1	hydrolysis					1342:1351	gradual acid hydrolysis	1329:1351	gradual acid hydrolysis of the polysaccharide	1329:1373	Structure information was elucidated by IR spectrum, GC-MS analysis after methylation and gradual acid hydrolysis of the polysaccharide.					
32289476	12	78	theme	mannan	1733:1738	arg1	core					1740:1743	a mannan core	1731:1743	a mannan core	1731:1743	The structure of SB2-1 was a branched polysaccharides composed of a mannan core and side chains consisted of glucose and galactose.					
32289476	18	79	theme	TNF-α	2553:2557	arg1	production					2497:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	18	79	theme	TNF-α	2553:2557	arg1	productions					2516:2526	the productions	2512:2526	the productions of IL-1, IL-6, IL-12 and TNF-α	2512:2557	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	11	80	from	22.2 kDa	1581:1588	arg1	heteropolysaccharide					1535:1554	a neutral heteropolysaccharide	1525:1554	a neutral heteropolysaccharide with molecular weight	1525:1576	RESULTS Physicochemical and structural analyses showed that SB2-1 was a neutral heteropolysaccharide with molecular weight at 22.2 kDa and consisted of glucose, galactose and mannose at a ratio of 2.0:1.5:1.0.					
32289476	11	80	from	22.2 kDa	1581:1588	arg1	SB2-1					1515:1519	SB2-1	1515:1519	SB2-1	1515:1519	RESULTS Physicochemical and structural analyses showed that SB2-1 was a neutral heteropolysaccharide with molecular weight at 22.2 kDa and consisted of glucose, galactose and mannose at a ratio of 2.0:1.5:1.0.					
32289476	2	81	theme	large	258:262	arg1	stroma					264:269	Its relatively large stroma	243:269	Its relatively large stroma	243:269	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	14	82	theme	polymerization	1940:1953	arg1	degrees					1929:1935	different degrees	1919:1935	different degrees of polymerization	1919:1953	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	20	83	from	polysaccharides	2863:2877	arg1	bambusicola					2885:2895	S. bambusicola	2882:2895	S. bambusicola	2882:2895	CONCLUSIONS The investigation demonstrated that the galactofuranose-containing mannogalactoglucan was characteristic polysaccharides in S. bambusicola and could enhance the activation of macrophages.					
32289476	0	84	from	bambusicola	132:142	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	0	84	from	bambusicola	132:142	arg1	activity					32:39	immunostimulating activity	14:39	immunostimulating activity	14:39	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	0	84	from	bambusicola	132:142	arg1	polysaccharide					67:80	a galactofuranose-rich polysaccharide	44:80	a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola	44:142	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	10	85	used	used	1400:1403	arg2	RAW264.7 cells					1380:1393	The RAW264.7 cells	1376:1393	The RAW264.7 cells	1376:1393	The RAW264.7 cells were used to study the immunostimulating activity in vitro.					
32289476	15	86	theme	terminal	2086:2093	arg1	1→					2102:2103	terminal D-Galf(1→	2086:2103	terminal D-Galf(1→	2086:2103	The galactose with the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→ also existed in the side chain.					
32289476	5	87	theme	immunostimulating	765:781	arg1	activity					783:790	its immunostimulating activity	761:790	its immunostimulating activity	761:790	To explore bioactive polysaccharides from Shiraia bambusicola, a heteropolysaccharide SB2-1 was purified and obtained from S. bambusicola and its immunostimulating activity was researched.					
32289476	2	88	theme	medicinal	280:288	arg1	value					290:294	high medicinal value	275:294	high medicinal value	275:294	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	7	89	theme	gel	1072:1074	arg1	chromatography					1087:1100	high performance gel permeation chromatography	1055:1100	high performance gel permeation chromatography	1055:1100	Molecular weight of polysaccharide was estimated by high performance gel permeation chromatography.					
32289476	0	90	from	activity	32:39	arg1	bambusicola					132:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	20	91	theme	macrophages	2933:2943	arg1	activation					2919:2928	the activation	2915:2928	the activation of macrophages	2915:2943	CONCLUSIONS The investigation demonstrated that the galactofuranose-containing mannogalactoglucan was characteristic polysaccharides in S. bambusicola and could enhance the activation of macrophages.					
32289476	0	92	theme	parasite	98:105	arg1	bambusicola					132:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	7	93	theme	high	1055:1058	arg1	chromatography					1087:1100	high performance gel permeation chromatography	1055:1100	high performance gel permeation chromatography	1055:1100	Molecular weight of polysaccharide was estimated by high performance gel permeation chromatography.					
32289476	14	94	with	Glucose	1859:1865	arg1	-D-Glcp					1908:1914	(1→6)-D-Glcp	1903:1914	(1→6)-D-Glcp	1903:1914	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	14	94	with	Glucose	1859:1865	arg1	-D-Glcp					1891:1897	(1→2)-D-Glcp	1886:1897	(1→2)-D-Glcp	1886:1897	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	14	94	with	Glucose	1859:1865	arg1	-D-Glcp					1877:1883	(1→4)-D-Glcp	1872:1883	(1→4)-D-Glcp	1872:1883	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	12	95	theme	SB2-1	1682:1686	arg1	polysaccharides					1703:1717	a branched polysaccharides	1692:1717	a branched polysaccharides composed of a mannan core and side chains consisted of glucose and galactose	1692:1794	The structure of SB2-1 was a branched polysaccharides composed of a mannan core and side chains consisted of glucose and galactose.					
32289476	12	95	theme	SB2-1	1682:1686	arg1	structure					1669:1677	The structure	1665:1677	The structure of SB2-1	1665:1686	The structure of SB2-1 was a branched polysaccharides composed of a mannan core and side chains consisted of glucose and galactose.					
32289476	11	96	theme	2.0:1.5:1.0	1652:1662	arg1	ratio					1643:1647	a ratio	1641:1647	a ratio of 2.0:1.5:1.0	1641:1662	RESULTS Physicochemical and structural analyses showed that SB2-1 was a neutral heteropolysaccharide with molecular weight at 22.2 kDa and consisted of glucose, galactose and mannose at a ratio of 2.0:1.5:1.0.					
32289476	0	97	theme	fungus	117:122	arg1	bambusicola					132:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	the bamboo parasite medicinal fungus Shiraia bambusicola	87:142	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	19	98	theme	important	2694:2702	arg1	role					2704:2707	an important role	2691:2707	an important role	2691:2707	The effect of SB2-1 was better than its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities.					
32289476	15	99	theme	-D-Galf	2071:2077	arg1	1→					2079:2080	(1→6)-D-Galf, →2)-D-Galf(1→	2054:2080	1→	2079:2080	The galactose with the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→ also existed in the side chain.					
32289476	1	100	dep	Shiraia	176:182	arg1	bambusicola					184:194	bambusicola	184:194	bambusicola	184:194	ETHNOPHARMACOLOGICAL RELEVANCE Shiraia bambusicola is a parasitic fungus on the twigs of bamboos.					
32289476	16	101	theme	P-2	2215:2217	arg1	activities					2172:2181	the immunostimulating activities	2150:2181	the immunostimulating activities of SB2-1 and its core structure P-2	2150:2217	The study on the immunostimulating activities of SB2-1 and its core structure P-2 were investigated on RAW264.7 macrophages.					
32289476	3	102	theme	Southern	482:489	arg1	China					491:495	Southern China	482:495	Southern China	482:495	It is widely distributed in many provinces in Southern China and also is also found in Japan.					
32289476	16	103	from	study	2141:2145	arg1	activities					2172:2181	the immunostimulating activities	2150:2181	the immunostimulating activities of SB2-1 and its core structure P-2	2150:2217	The study on the immunostimulating activities of SB2-1 and its core structure P-2 were investigated on RAW264.7 macrophages.					
32289476	8	104	theme	performance	1155:1165	arg1	chromatography					1174:1187	high performance liquid chromatography	1150:1187	high performance liquid chromatography	1150:1187	Monosaccharide compositions were determined by high performance liquid chromatography after pre-column derivatization and UV detection.					
32289476	7	105	theme	polysaccharide	1023:1036	arg1	weight					1013:1018	Molecular weight	1003:1018	Molecular weight of polysaccharide	1003:1036	Molecular weight of polysaccharide was estimated by high performance gel permeation chromatography.					
32289476	19	106	theme	structure	2605:2613	arg1	P-2					2615:2617	its core structure P-2	2596:2617	its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities	2596:2743	The effect of SB2-1 was better than its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities.					
32289476	6	107	from	polysaccharide	834:847	arg1	METHODS					822:828	METHODS	822:828	METHODS	822:828	MATERIALS AND METHODS The polysaccharide from S. bambusicola was extracted and purified using enzyme assisted extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.					
32289476	6	107	from	polysaccharide	834:847	arg1	MATERIALS					808:816	MATERIALS	808:816	MATERIALS	808:816	MATERIALS AND METHODS The polysaccharide from S. bambusicola was extracted and purified using enzyme assisted extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.					
32289476	15	108	theme	-D-Galf	2059:2065	arg1	1→					2079:2080	(1→6)-D-Galf, →2)-D-Galf(1→	2054:2080	1→	2079:2080	The galactose with the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→ also existed in the side chain.					
32289476	19	109	theme	core	2600:2603	arg1	structure					2605:2613	its core structure	2596:2613	its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities	2596:2743	The effect of SB2-1 was better than its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities.					
32289476	17	110	theme	neutral	2379:2385	arg1	experiment					2398:2407	neutral red uptake experiment	2379:2407	neutral red uptake experiment	2379:2407	The results showed that SB2-1 could activate RAW264.7 macrophage and significantly improve its phagocytic ability by neutral red uptake experiment.					
32289476	13	111	theme	1→2	1830:1832	arg1	-Manp					1834:1838	(1→2)-Manp	1829:1838	(1→2)-Manp	1829:1838	The mannan core was composed of (1→2)-Manp as the main chain.					
32289476	16	112	theme	RAW264.7	2240:2247	arg1	macrophages					2249:2259	RAW264.7 macrophages	2240:2259	RAW264.7 macrophages	2240:2259	The study on the immunostimulating activities of SB2-1 and its core structure P-2 were investigated on RAW264.7 macrophages.					
32289476	8	113	theme	pre-column	1195:1204	arg1	derivatization					1206:1219	pre-column derivatization	1195:1219	pre-column derivatization	1195:1219	Monosaccharide compositions were determined by high performance liquid chromatography after pre-column derivatization and UV detection.					
32289476	19	114	theme	gradual	2631:2637	arg1	hydrolysis					2644:2653	gradual acid hydrolysis	2631:2653	gradual acid hydrolysis	2631:2653	The effect of SB2-1 was better than its core structure P-2 produced by gradual acid hydrolysis, which meant the side chains played an important role in the immunostimulating activities.					
32289476	15	115	dep	1→	2079:2080	arg1	1→6					2055:2057	1→6	2055:2057	1→6	2055:2057	The galactose with the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→ also existed in the side chain.					
32289476	15	115	dep	1→	2079:2080	arg1	→2					2068:2069	(1→6)-D-Galf, →2)-D-Galf(1→	2054:2080	→2	2068:2069	The galactose with the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→ also existed in the side chain.					
32289476	4	116	theme	Medicinal	547:555	arg1	fungi					557:561	THE STUDY Medicinal fungi	537:561	THE STUDY Medicinal fungi	537:561	AIM OF THE STUDY Medicinal fungi were important resources for bioactive polysaccharides.					
32289476	13	117	theme	mannan	1801:1806	arg1	core					1808:1811	The mannan core	1797:1811	The mannan core	1797:1811	The mannan core was composed of (1→2)-Manp as the main chain.					
32289476	13	117	theme	mannan	1801:1806	arg1	chain					1852:1856	the main chain	1843:1856	the main chain	1843:1856	The mannan core was composed of (1→2)-Manp as the main chain.					
32289476	18	118	theme	higher	2451:2456	arg1	production					2497:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	16	119	theme	structure	2205:2213	arg1	P-2					2215:2217	its core structure P-2	2196:2217	its core structure P-2	2196:2217	The study on the immunostimulating activities of SB2-1 and its core structure P-2 were investigated on RAW264.7 macrophages.					
32289476	6	120	theme	enzyme	902:907	arg1	extraction					918:927	enzyme assisted extraction	902:927	enzyme assisted extraction	902:927	MATERIALS AND METHODS The polysaccharide from S. bambusicola was extracted and purified using enzyme assisted extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.					
32289476	20	121	dep	CONCLUSIONS	2746:2756	arg1	demonstrated					2776:2787	demonstrated	2776:2787	demonstrated that the galactofuranose-containing mannogalactoglucan was characteristic polysaccharides in S. bambusicola and could enhance the activation of macrophages	2776:2943	CONCLUSIONS The investigation demonstrated that the galactofuranose-containing mannogalactoglucan was characteristic polysaccharides in S. bambusicola and could enhance the activation of macrophages.					
32289476	0	122	theme	polysaccharide	67:80	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	0	122	theme	polysaccharide	67:80	arg1	activity					32:39	immunostimulating activity	14:39	immunostimulating activity	14:39	Structure and immunostimulating activity of a galactofuranose-rich polysaccharide from the bamboo parasite medicinal fungus Shiraia bambusicola.					
32289476	11	123	dep	RESULTS	1455:1461	arg1	Physicochemical					1463:1477	Physicochemical	1463:1477	Physicochemical	1463:1477	RESULTS Physicochemical and structural analyses showed that SB2-1 was a neutral heteropolysaccharide with molecular weight at 22.2 kDa and consisted of glucose, galactose and mannose at a ratio of 2.0:1.5:1.0.					
32289476	11	123	dep	RESULTS	1455:1461	arg1	analyses					1494:1501	structural analyses	1483:1501	structural analyses	1483:1501	RESULTS Physicochemical and structural analyses showed that SB2-1 was a neutral heteropolysaccharide with molecular weight at 22.2 kDa and consisted of glucose, galactose and mannose at a ratio of 2.0:1.5:1.0.					
32289476	18	124	theme	synthase	2481:2488	arg1	production					2497:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	14	125	from	degrees	1929:1935	arg1	Glucose					1859:1865	Glucose	1859:1865	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization	1859:1953	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	14	126	theme	-Manp	1994:1998	arg1	C-3					1978:1980	C-3	1978:1980	C-3	1978:1980	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	14	126	theme	-Manp	1994:1998	arg1	C-6					1970:1972	C-6	1970:1972	C-6	1970:1972	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	4	127	theme	bioactive	592:600	arg1	polysaccharides					602:616	bioactive polysaccharides	592:616	bioactive polysaccharides	592:616	AIM OF THE STUDY Medicinal fungi were important resources for bioactive polysaccharides.					
32289476	18	128	theme	nitric	2468:2473	arg1	iNOS					2491:2494	iNOS	2491:2494	iNOS	2491:2494	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	18	128	theme	nitric	2468:2473	arg1	synthase					2481:2488	inducible nitric oxide synthase	2458:2488	significantly higher inducible nitric oxide synthase (iNOS) production	2437:2506	Meanwhile, SB2-1 increased significantly higher inducible nitric oxide synthase (iNOS) production and the productions of IL-1, IL-6, IL-12 and TNF-α.					
32289476	2	129	theme	rheumatoid	340:349	arg1	arthritis					351:359	rheumatoid arthritis	340:359	rheumatoid arthritis	340:359	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	15	130	theme	1→	2079:2080	arg1	linages					2043:2049	the linages	2039:2049	the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→	2039:2103	The galactose with the linages of (1→6)-D-Galf, →2)-D-Galf(1→ and terminal D-Galf(1→ also existed in the side chain.					
32289476	14	131	dep	-D-Glcp	1908:1914	arg1	1→6					1904:1906	1→6	1904:1906	1→6	1904:1906	Glucose with (1→4)-D-Glcp, (1→2)-D-Glcp and (1→6)-D-Glcp at different degrees of polymerization were linked at C-6 and C-3 of the (1→2)-Manp as the side chains.					
32289476	2	132	contain	has	271:273	arg1	stroma					264:269	Its relatively large stroma	243:269	Its relatively large stroma	243:269	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32289476	2	132	contain	has	271:273	arg2	value					290:294	high medicinal value	275:294	high medicinal value	275:294	Its relatively large stroma has high medicinal value and can treat a variety of diseases such as rheumatoid arthritis, cold stomach pain, sciatica, injuries, chronic bronchitis, and infantile.					
32827618	5	0	theme	swelling	836:843	arg1	superiority					859:869	superiority	859:869	superiority	859:869	With high amount of hyaluronic acid, the swelling behaviour was superiority.					
32827618	5	0	theme	swelling	836:843	arg1	behaviour					845:853	the swelling behaviour	832:853	the swelling behaviour	832:853	With high amount of hyaluronic acid, the swelling behaviour was superiority.					
32827618	6	1	theme	%	997:997	arg1	release					978:984	a gradual release	968:984	a gradual release of up to 80% by 480 min	968:1008	The hydrogels showed an initial burst release of caffeic acid (~70%) within 60 min, followed by a gradual release of up to 80% by 480 min.					
32827618	2	2	theme	acid	402:405	arg1	hydrogels					407:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels were characterized by X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis.					
32827618	8	3	theme	FRAP	1111:1114	arg1	assays					1116:1121	DPPH, ABTS+, and FRAP assays	1094:1121	DPPH, ABTS+, and FRAP assays	1094:1121	In addition, DPPH, ABTS+, and FRAP assays revealed that the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels exhibited antioxidant activity.					
32827618	0	4	theme	acid	98:101	arg1	addition					103:110	caffeic acid addition	90:110	caffeic acid addition	90:110	Preparation of chitosan/hydrolyzed collagen/hyaluronic acid based hydrogel composite with caffeic acid addition.					
32827618	6	5	theme	initial	896:902	arg1	release					910:916	an initial burst release	893:916	an initial burst release of caffeic acid (~70%)	893:939	The hydrogels showed an initial burst release of caffeic acid (~70%) within 60 min, followed by a gradual release of up to 80% by 480 min.					
32827618	1	6	theme	hyaluronic	175:184	arg1	acid					186:189	hyaluronic acid	175:189	hyaluronic acid	175:189	In this work, biopolymer hydrogels were synthesized by mixing hyaluronic acid, hydrolyzed collagen, and chitosan through a solvent evaporation method and incorporating them with caffeic acid as an antioxidant agent.					
32827618	8	7	theme	acid/hydrolyzed	1172:1186	arg1	hydrogels					1206:1214	the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels	1137:1214	the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels	1137:1214	In addition, DPPH, ABTS+, and FRAP assays revealed that the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels exhibited antioxidant activity.					
32827618	4	8	theme	hydrogels	683:691	arg1	smooth					697:702	smooth	697:702	smooth	697:702	Furthermore, scanning electron microscope reported that the surface morphology of the hydrogels was smooth, and no significant change in porosity was observed after the addition of hyaluronic acid.					
32827618	4	8	theme	hydrogels	683:691	arg1	morphology					665:674	the surface morphology	653:674	the surface morphology of the hydrogels	653:691	Furthermore, scanning electron microscope reported that the surface morphology of the hydrogels was smooth, and no significant change in porosity was observed after the addition of hyaluronic acid.					
32827618	8	9	theme	hyaluronic	1161:1170	arg1	hydrogels					1206:1214	the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels	1137:1214	the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels	1137:1214	In addition, DPPH, ABTS+, and FRAP assays revealed that the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels exhibited antioxidant activity.					
32827618	4	10	theme	hyaluronic	778:787	arg1	acid					789:792	hyaluronic acid	778:792	hyaluronic acid	778:792	Furthermore, scanning electron microscope reported that the surface morphology of the hydrogels was smooth, and no significant change in porosity was observed after the addition of hyaluronic acid.					
32827618	9	11	with	materials	1309:1317	arg1	activity					1336:1343	antioxidant activity	1324:1343	antioxidant activity	1324:1343	Thus, these composites could potentially be used as dressing materials with antioxidant activity.					
32827618	5	12	theme	hyaluronic	815:824	arg1	acid					826:829	hyaluronic acid	815:829	hyaluronic acid	815:829	With high amount of hyaluronic acid, the swelling behaviour was superiority.					
32827618	3	13	theme	thermal	564:570	arg1	properties					572:581	structural and thermal properties	549:581	structural and thermal properties	549:581	No significant change on structural and thermal properties was observed.					
32827618	5	14	theme	acid	826:829	arg1	amount					805:810	high amount	800:810	high amount of hyaluronic acid	800:829	With high amount of hyaluronic acid, the swelling behaviour was superiority.					
32827618	5	14	theme	acid	826:829	arg1	acid					826:829	hyaluronic acid	815:829	hyaluronic acid	815:829	With high amount of hyaluronic acid, the swelling behaviour was superiority.					
32827618	5	15	with	superiority	859:869	arg1	amount					805:810	high amount	800:810	high amount of hyaluronic acid	800:829	With high amount of hyaluronic acid, the swelling behaviour was superiority.					
32827618	5	15	with	superiority	859:869	arg1	acid					826:829	hyaluronic acid	815:829	hyaluronic acid	815:829	With high amount of hyaluronic acid, the swelling behaviour was superiority.					
32827618	2	16	theme	X-ray	439:443	arg1	diffraction					445:455	X-ray diffraction	439:455	X-ray diffraction	439:455	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels were characterized by X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis.					
32827618	8	17	theme	antioxidant	1226:1236	arg1	activity					1238:1245	antioxidant activity	1226:1245	antioxidant activity	1226:1245	In addition, DPPH, ABTS+, and FRAP assays revealed that the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels exhibited antioxidant activity.					
32827618	8	18	theme	caffeic	1141:1147	arg1	hydrogels					1206:1214	the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels	1137:1214	the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels	1137:1214	In addition, DPPH, ABTS+, and FRAP assays revealed that the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels exhibited antioxidant activity.					
32827618	7	19	theme	acid	1075:1078	arg1	presence					1052:1059	the presence	1048:1059	the presence of hyaluronic acid	1048:1078	The release was slightly higher with the presence of hyaluronic acid.					
32827618	1	20	theme	hydrolyzed	192:201	arg1	collagen					203:210	hydrolyzed collagen	192:210	hydrolyzed collagen	192:210	In this work, biopolymer hydrogels were synthesized by mixing hyaluronic acid, hydrolyzed collagen, and chitosan through a solvent evaporation method and incorporating them with caffeic acid as an antioxidant agent.					
32827618	1	21	theme	caffeic	291:297	arg1	acid					299:302	caffeic acid	291:302	caffeic acid	291:302	In this work, biopolymer hydrogels were synthesized by mixing hyaluronic acid, hydrolyzed collagen, and chitosan through a solvent evaporation method and incorporating them with caffeic acid as an antioxidant agent.					
32827618	9	22	used	used	1292:1295	arg2	composites					1260:1269	these composites	1254:1269	these composites	1254:1269	Thus, these composites could potentially be used as dressing materials with antioxidant activity.					
32827618	9	22	used	used	1292:1295	arg2	materials					1309:1317	dressing materials	1300:1317	dressing materials with antioxidant activity	1300:1343	Thus, these composites could potentially be used as dressing materials with antioxidant activity.					
32827618	8	23	theme	acid-loaded	1149:1159	arg1	hydrogels					1206:1214	the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels	1137:1214	the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels	1137:1214	In addition, DPPH, ABTS+, and FRAP assays revealed that the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels exhibited antioxidant activity.					
32827618	0	24	theme	collagen/hyaluronic	35:53	arg1	acid					55:58	chitosan/hydrolyzed collagen/hyaluronic acid	15:58	chitosan/hydrolyzed collagen/hyaluronic acid	15:58	Preparation of chitosan/hydrolyzed collagen/hyaluronic acid based hydrogel composite with caffeic acid addition.					
32827618	8	25	theme	collagen/chitosan	1188:1204	arg1	hydrogels					1206:1214	the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels	1137:1214	the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels	1137:1214	In addition, DPPH, ABTS+, and FRAP assays revealed that the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels exhibited antioxidant activity.					
32827618	4	26	from	change	724:729	arg1	porosity					734:741	porosity	734:741	porosity	734:741	Furthermore, scanning electron microscope reported that the surface morphology of the hydrogels was smooth, and no significant change in porosity was observed after the addition of hyaluronic acid.					
32827618	4	27	theme	significant	712:722	arg1	change					724:729	no significant change	709:729	no significant change in porosity	709:741	Furthermore, scanning electron microscope reported that the surface morphology of the hydrogels was smooth, and no significant change in porosity was observed after the addition of hyaluronic acid.					
32827618	0	28	theme	chitosan/hydrolyzed	15:33	arg1	acid					55:58	chitosan/hydrolyzed collagen/hyaluronic acid	15:58	chitosan/hydrolyzed collagen/hyaluronic acid	15:58	Preparation of chitosan/hydrolyzed collagen/hyaluronic acid based hydrogel composite with caffeic acid addition.					
32827618	4	29	theme	surface	657:663	arg1	smooth					697:702	smooth	697:702	smooth	697:702	Furthermore, scanning electron microscope reported that the surface morphology of the hydrogels was smooth, and no significant change in porosity was observed after the addition of hyaluronic acid.					
32827618	4	29	theme	surface	657:663	arg1	morphology					665:674	the surface morphology	653:674	the surface morphology of the hydrogels	653:691	Furthermore, scanning electron microscope reported that the surface morphology of the hydrogels was smooth, and no significant change in porosity was observed after the addition of hyaluronic acid.					
32827618	8	30	theme	DPPH	1094:1097	arg1	assays					1116:1121	DPPH, ABTS+, and FRAP assays	1094:1121	DPPH, ABTS+, and FRAP assays	1094:1121	In addition, DPPH, ABTS+, and FRAP assays revealed that the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels exhibited antioxidant activity.					
32827618	2	31	theme	collagen/hyaluronic	382:400	arg1	hydrogels					407:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels were characterized by X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis.					
32827618	2	32	theme	scanning	471:478	arg1	calorimetry					480:490	differential scanning calorimetry	458:490	differential scanning calorimetry	458:490	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels were characterized by X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis.					
32827618	2	33	theme	acid-loaded	350:360	arg1	hydrogels					407:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels were characterized by X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis.					
32827618	2	34	theme	differential	458:469	arg1	calorimetry					480:490	differential scanning calorimetry	458:490	differential scanning calorimetry	458:490	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels were characterized by X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis.					
32827618	0	35	theme	acid	55:58	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan/hydrolyzed collagen/hyaluronic acid	0:58	Preparation of chitosan/hydrolyzed collagen/hyaluronic acid based hydrogel composite with caffeic acid addition.					
32827618	1	36	theme	antioxidant	310:320	arg1	agent					322:326	an antioxidant agent	307:326	an antioxidant agent	307:326	In this work, biopolymer hydrogels were synthesized by mixing hyaluronic acid, hydrolyzed collagen, and chitosan through a solvent evaporation method and incorporating them with caffeic acid as an antioxidant agent.					
32827618	1	36	theme	antioxidant	310:320	arg1	them					281:284	them	281:284	them	281:284	In this work, biopolymer hydrogels were synthesized by mixing hyaluronic acid, hydrolyzed collagen, and chitosan through a solvent evaporation method and incorporating them with caffeic acid as an antioxidant agent.					
32827618	5	37	theme	high	800:803	arg1	amount					805:810	high amount	800:810	high amount of hyaluronic acid	800:829	With high amount of hyaluronic acid, the swelling behaviour was superiority.					
32827618	5	37	theme	high	800:803	arg1	acid					826:829	hyaluronic acid	815:829	hyaluronic acid	815:829	With high amount of hyaluronic acid, the swelling behaviour was superiority.					
32827618	2	38	theme	thermogravimetric	496:512	arg1	analysis					514:521	thermogravimetric analysis	496:521	thermogravimetric analysis	496:521	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels were characterized by X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis.					
32827618	3	39	theme	significant	527:537	arg1	change					539:544	No significant change	524:544	No significant change on structural and thermal properties	524:581	No significant change on structural and thermal properties was observed.					
32827618	4	40	theme	scanning	610:617	arg1	microscope					628:637	scanning electron microscope	610:637	scanning electron microscope	610:637	Furthermore, scanning electron microscope reported that the surface morphology of the hydrogels was smooth, and no significant change in porosity was observed after the addition of hyaluronic acid.					
32827618	7	41	theme	hyaluronic	1064:1073	arg1	acid					1075:1078	hyaluronic acid	1064:1078	hyaluronic acid	1064:1078	The release was slightly higher with the presence of hyaluronic acid.					
32827618	6	42	theme	acid	929:932	arg1	release					910:916	an initial burst release	893:916	an initial burst release of caffeic acid (~70%)	893:939	The hydrogels showed an initial burst release of caffeic acid (~70%) within 60 min, followed by a gradual release of up to 80% by 480 min.					
32827618	2	43	theme	chitosan/hydrolyzed	362:380	arg1	hydrogels					407:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels were characterized by X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis.					
32827618	0	44	theme	hydrogel	66:73	arg1	composite					75:83	hydrogel composite	66:83	hydrogel composite	66:83	Preparation of chitosan/hydrolyzed collagen/hyaluronic acid based hydrogel composite with caffeic acid addition.					
32827618	3	45	theme	structural	549:558	arg1	properties					572:581	structural and thermal properties	549:581	structural and thermal properties	549:581	No significant change on structural and thermal properties was observed.					
32827618	6	46	theme	caffeic	921:927	arg1	acid					929:932	caffeic acid	921:932	caffeic acid (~70%)	921:939	The hydrogels showed an initial burst release of caffeic acid (~70%) within 60 min, followed by a gradual release of up to 80% by 480 min.					
32827618	6	46	theme	caffeic	921:927	arg1	%					938:938	~70%	935:938	~70%	935:938	The hydrogels showed an initial burst release of caffeic acid (~70%) within 60 min, followed by a gradual release of up to 80% by 480 min.					
32827618	9	47	theme	dressing	1300:1307	arg1	materials					1309:1317	dressing materials	1300:1317	dressing materials with antioxidant activity	1300:1343	Thus, these composites could potentially be used as dressing materials with antioxidant activity.					
32827618	9	47	theme	dressing	1300:1307	arg1	composites					1260:1269	these composites	1254:1269	these composites	1254:1269	Thus, these composites could potentially be used as dressing materials with antioxidant activity.					
32827618	3	48	from	change	539:544	arg1	properties					572:581	structural and thermal properties	549:581	structural and thermal properties	549:581	No significant change on structural and thermal properties was observed.					
32827618	1	49	theme	biopolymer	127:136	arg1	hydrogels					138:146	biopolymer hydrogels	127:146	biopolymer hydrogels	127:146	In this work, biopolymer hydrogels were synthesized by mixing hyaluronic acid, hydrolyzed collagen, and chitosan through a solvent evaporation method and incorporating them with caffeic acid as an antioxidant agent.					
32827618	4	50	theme	electron	619:626	arg1	microscope					628:637	scanning electron microscope	610:637	scanning electron microscope	610:637	Furthermore, scanning electron microscope reported that the surface morphology of the hydrogels was smooth, and no significant change in porosity was observed after the addition of hyaluronic acid.					
32827618	6	51	dep	80	995:996	arg1	to					992:993	to	992:993	to	992:993	The hydrogels showed an initial burst release of caffeic acid (~70%) within 60 min, followed by a gradual release of up to 80% by 480 min.					
32827618	2	52	theme	caffeic	342:348	arg1	hydrogels					407:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels were characterized by X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis.					
32827618	2	53	theme	obtained	333:340	arg1	hydrogels					407:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels	329:415	The obtained caffeic acid-loaded chitosan/hydrolyzed collagen/hyaluronic acid hydrogels were characterized by X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis.					
32827618	1	54	theme	solvent	236:242	arg1	method					256:261	a solvent evaporation method	234:261	a solvent evaporation method	234:261	In this work, biopolymer hydrogels were synthesized by mixing hyaluronic acid, hydrolyzed collagen, and chitosan through a solvent evaporation method and incorporating them with caffeic acid as an antioxidant agent.					
32827618	0	55	theme	caffeic	90:96	arg1	addition					103:110	caffeic acid addition	90:110	caffeic acid addition	90:110	Preparation of chitosan/hydrolyzed collagen/hyaluronic acid based hydrogel composite with caffeic acid addition.					
32827618	6	56	theme	burst	904:908	arg1	release					910:916	an initial burst release	893:916	an initial burst release of caffeic acid (~70%)	893:939	The hydrogels showed an initial burst release of caffeic acid (~70%) within 60 min, followed by a gradual release of up to 80% by 480 min.					
32827618	1	57	theme	evaporation	244:254	arg1	method					256:261	a solvent evaporation method	234:261	a solvent evaporation method	234:261	In this work, biopolymer hydrogels were synthesized by mixing hyaluronic acid, hydrolyzed collagen, and chitosan through a solvent evaporation method and incorporating them with caffeic acid as an antioxidant agent.					
32827618	6	58	theme	gradual	970:976	arg1	release					978:984	a gradual release	968:984	a gradual release of up to 80% by 480 min	968:1008	The hydrogels showed an initial burst release of caffeic acid (~70%) within 60 min, followed by a gradual release of up to 80% by 480 min.					
32827618	9	59	theme	antioxidant	1324:1334	arg1	activity					1336:1343	antioxidant activity	1324:1343	antioxidant activity	1324:1343	Thus, these composites could potentially be used as dressing materials with antioxidant activity.					
32827618	8	60	theme	ABTS+	1100:1104	arg1	assays					1116:1121	DPPH, ABTS+, and FRAP assays	1094:1121	DPPH, ABTS+, and FRAP assays	1094:1121	In addition, DPPH, ABTS+, and FRAP assays revealed that the caffeic acid-loaded hyaluronic acid/hydrolyzed collagen/chitosan hydrogels exhibited antioxidant activity.					
32827618	4	61	theme	acid	789:792	arg1	addition					766:773	the addition	762:773	the addition of hyaluronic acid	762:792	Furthermore, scanning electron microscope reported that the surface morphology of the hydrogels was smooth, and no significant change in porosity was observed after the addition of hyaluronic acid.					
34097954	0	0	theme	mechanical	87:96	arg1	properties					98:107	improved mechanical properties	78:107	improved mechanical properties	78:107	Melt-processed poly (vinyl alcohol)/corn starch/nanocellulose composites with improved mechanical properties.					
34097954	3	1	theme	infrared	487:494	arg1	spectroscopy					496:507	infrared spectroscopy	487:507	infrared spectroscopy	487:507	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	0	2	theme	improved	78:85	arg1	properties					98:107	improved mechanical properties	78:107	improved mechanical properties	78:107	Melt-processed poly (vinyl alcohol)/corn starch/nanocellulose composites with improved mechanical properties.					
34097954	5	3	from	break	883:887	arg1	elongation					869:878	elongation	869:878	elongation	869:878	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	5	3	from	break	883:887	arg1	modulus					857:863	modulus	857:863	modulus	857:863	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	5	3	from	break	883:887	arg1	strength					847:854	The tensile strength	835:854	The tensile strength	835:854	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	5	4	theme	10 wt	988:992	arg1	%					993:993	10 wt%	988:993	10 wt%	988:993	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	3	5	theme	intra-	554:559	arg1	bonds					590:594	the intra- and inter-molecular hydrogen bonds	550:594	the intra- and inter-molecular hydrogen bonds of PVA	550:601	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	6	6	theme	PVA	1111:1113	arg1	products					1099:1106	complex three-dimensional products	1073:1106	complex three-dimensional products of PVA	1073:1113	This strategy is not only expected to provide a direction for preparing complex three-dimensional products of PVA by melt-processing, but also provide a method to enhance the mechanical properties of other biodegradable plastics.					
34097954	3	7	theme	melt-processing	700:714	arg1	treatment					716:724	the melt-processing treatment	696:724	the melt-processing treatment	696:724	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	6	8	theme	plastics	1221:1228	arg1	properties					1187:1196	the mechanical properties	1172:1196	the mechanical properties of other biodegradable plastics	1172:1228	This strategy is not only expected to provide a direction for preparing complex three-dimensional products of PVA by melt-processing, but also provide a method to enhance the mechanical properties of other biodegradable plastics.					
34097954	0	9	with	composites	62:71	arg1	properties					98:107	improved mechanical properties	78:107	improved mechanical properties	78:107	Melt-processed poly (vinyl alcohol)/corn starch/nanocellulose composites with improved mechanical properties.					
34097954	3	10	dep	Fourier	469:475	arg1	transform					477:485	transform	477:485	transform infrared spectroscopy	477:507	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	3	10	dep	Fourier	469:475	arg1	analysis					527:534	analysis	527:534	analysis	527:534	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	6	11	theme	biodegradable	1207:1219	arg1	plastics					1221:1228	other biodegradable plastics	1201:1228	other biodegradable plastics	1201:1228	This strategy is not only expected to provide a direction for preparing complex three-dimensional products of PVA by melt-processing, but also provide a method to enhance the mechanical properties of other biodegradable plastics.					
34097954	2	12	theme	composites	457:466	arg1	thermoplasticity					433:448	the thermoplasticity	429:448	the thermoplasticity of PVA composites	429:466	Based on the regulation and control of hydrogen bonding network, CS and CNFs can extend the processing window and improve the thermoplasticity of PVA composites.					
34097954	6	13	theme	other	1201:1205	arg1	plastics					1221:1228	other biodegradable plastics	1201:1228	other biodegradable plastics	1201:1228	This strategy is not only expected to provide a direction for preparing complex three-dimensional products of PVA by melt-processing, but also provide a method to enhance the mechanical properties of other biodegradable plastics.					
34097954	0	14	theme	Melt-processed	0:13	arg1	poly					15:18	Melt-processed poly	0:18	Melt-processed poly (vinyl alcohol)	0:34	Melt-processed poly (vinyl alcohol)/corn starch/nanocellulose composites with improved mechanical properties.					
34097954	0	14	theme	Melt-processed	0:13	arg1	alcohol					27:33	vinyl alcohol	21:33	vinyl alcohol	21:33	Melt-processed poly (vinyl alcohol)/corn starch/nanocellulose composites with improved mechanical properties.					
34097954	1	15	theme	robust	248:253	arg1	composites					271:280	mechanically robust and sustainable composites	235:280	mechanically robust and sustainable composites	235:280	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	3	16	theme	inter-molecular	565:579	arg1	bonds					590:594	the intra- and inter-molecular hydrogen bonds	550:594	the intra- and inter-molecular hydrogen bonds of PVA	550:601	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	2	17	theme	network	363:369	arg1	regulation					320:329	regulation	320:329	regulation	320:329	Based on the regulation and control of hydrogen bonding network, CS and CNFs can extend the processing window and improve the thermoplasticity of PVA composites.					
34097954	2	17	theme	network	363:369	arg1	control					335:341	control	335:341	control	335:341	Based on the regulation and control of hydrogen bonding network, CS and CNFs can extend the processing window and improve the thermoplasticity of PVA composites.					
34097954	0	18	theme	vinyl	21:25	arg1	poly					15:18	Melt-processed poly	0:18	Melt-processed poly (vinyl alcohol)	0:34	Melt-processed poly (vinyl alcohol)/corn starch/nanocellulose composites with improved mechanical properties.					
34097954	0	18	theme	vinyl	21:25	arg1	alcohol					27:33	vinyl alcohol	21:33	vinyl alcohol	21:33	Melt-processed poly (vinyl alcohol)/corn starch/nanocellulose composites with improved mechanical properties.					
34097954	1	19	theme	Corn	110:113	arg1	CS					123:124	CS	123:124	CS	123:124	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	1	19	theme	Corn	110:113	arg1	starch					115:120	Corn starch	110:120	Corn starch (CS)	110:125	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	5	20	theme	CS	981:982	arg1	%					979:979	10 wt% CS	974:982	10 wt% CS	974:982	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	1	21	theme	biodegradable	183:195	arg1	poly					197:200	biodegradable poly	183:200	biodegradable poly (vinyl alcohol) (PVA)	183:222	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	1	21	theme	biodegradable	183:195	arg1	alcohol					209:215	vinyl alcohol	203:215	vinyl alcohol	203:215	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	1	21	theme	biodegradable	183:195	arg1	PVA					219:221	PVA	219:221	PVA	219:221	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	2	22	theme	processing	399:408	arg1	window					410:415	the processing window	395:415	the processing window	395:415	Based on the regulation and control of hydrogen bonding network, CS and CNFs can extend the processing window and improve the thermoplasticity of PVA composites.					
34097954	1	23	theme	sustainable	259:269	arg1	composites					271:280	mechanically robust and sustainable composites	235:280	mechanically robust and sustainable composites	235:280	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	4	24	theme	processing	759:768	arg1	window					770:775	the processing window	755:775	the processing window of PVA composite	755:792	Thermal analysis shows that the processing window of PVA composite is significantly broadened to 131.46 °C.					
34097954	2	25	dep	regulation	320:329	arg1	the					316:318	the	316:318	the	316:318	Based on the regulation and control of hydrogen bonding network, CS and CNFs can extend the processing window and improve the thermoplasticity of PVA composites.					
34097954	1	26	theme	cellulose	131:139	arg1	nanofibrils					141:151	cellulose nanofibrils	131:151	cellulose nanofibrils (CNFs)	131:158	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	1	26	theme	cellulose	131:139	arg1	CNFs					154:157	CNFs	154:157	CNFs	154:157	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	2	27	theme	PVA	453:455	arg1	composites					457:466	PVA composites	453:466	PVA composites	453:466	Based on the regulation and control of hydrogen bonding network, CS and CNFs can extend the processing window and improve the thermoplasticity of PVA composites.					
34097954	6	28	theme	complex	1073:1079	arg1	products					1099:1106	complex three-dimensional products	1073:1106	complex three-dimensional products of PVA	1073:1113	This strategy is not only expected to provide a direction for preparing complex three-dimensional products of PVA by melt-processing, but also provide a method to enhance the mechanical properties of other biodegradable plastics.					
34097954	5	29	theme	CNFs	995:998	arg1	incorporation					957:969	the incorporation	953:969	the incorporation of 10 wt% CS and 10 wt% CNFs	953:998	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	3	30	dep	broken	607:612	arg1	accompanied					615:625	accompanied	615:625	accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment	615:724	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	3	31	theme	PVA	599:601	arg1	bonds					590:594	the intra- and inter-molecular hydrogen bonds	550:594	the intra- and inter-molecular hydrogen bonds of PVA	550:601	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	5	32	theme	tensile	839:845	arg1	strength					847:854	The tensile strength	835:854	The tensile strength	835:854	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	1	33	theme	vinyl	203:207	arg1	poly					197:200	biodegradable poly	183:200	biodegradable poly (vinyl alcohol) (PVA)	183:222	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	1	33	theme	vinyl	203:207	arg1	alcohol					209:215	vinyl alcohol	203:215	vinyl alcohol	203:215	Corn starch (CS) and cellulose nanofibrils (CNFs) were incorporated into biodegradable poly (vinyl alcohol) (PVA) to prepare mechanically robust and sustainable composites through melt-processing.					
34097954	0	34	theme	starch/nanocellulose	41:60	arg1	composites					62:71	starch/nanocellulose composites	41:71	starch/nanocellulose composites with improved mechanical properties	41:107	Melt-processed poly (vinyl alcohol)/corn starch/nanocellulose composites with improved mechanical properties.					
34097954	3	35	theme	hydrogen	581:588	arg1	bonds					590:594	the intra- and inter-molecular hydrogen bonds	550:594	the intra- and inter-molecular hydrogen bonds of PVA	550:601	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	6	36	theme	three-dimensional	1081:1097	arg1	products					1099:1106	complex three-dimensional products	1073:1106	complex three-dimensional products of PVA	1073:1113	This strategy is not only expected to provide a direction for preparing complex three-dimensional products of PVA by melt-processing, but also provide a method to enhance the mechanical properties of other biodegradable plastics.					
34097954	3	37	theme	bonds	660:664	arg1	formation					634:642	the formation	630:642	the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment	630:724	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	5	38	theme	%	993:993	arg1	CNFs					995:998	10 wt% CS and 10 wt% CNFs	974:998	10 wt% CS and 10 wt% CNFs	974:998	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	5	39	theme	composites	896:905	arg1	elongation					869:878	elongation	869:878	elongation	869:878	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	5	39	theme	composites	896:905	arg1	modulus					857:863	modulus	857:863	modulus	857:863	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	5	39	theme	composites	896:905	arg1	strength					847:854	The tensile strength	835:854	The tensile strength	835:854	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	5	40	theme	%	979:979	arg1	CNFs					995:998	10 wt% CS and 10 wt% CNFs	974:998	10 wt% CS and 10 wt% CNFs	974:998	The tensile strength, modulus and elongation at break of the composites reach to 28.19 MPa, 1572.54 MPa and 10.72% by the incorporation of 10 wt% CS and 10 wt% CNFs.					
34097954	6	41	theme	mechanical	1176:1185	arg1	properties					1187:1196	the mechanical properties	1172:1196	the mechanical properties of other biodegradable plastics	1172:1228	This strategy is not only expected to provide a direction for preparing complex three-dimensional products of PVA by melt-processing, but also provide a method to enhance the mechanical properties of other biodegradable plastics.					
34097954	3	42	theme	new	647:649	arg1	bonds					660:664	new hydrogen bonds	647:664	new hydrogen bonds among PVA, CS and CNFs	647:687	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	4	43	theme	Thermal	727:733	arg1	analysis					735:742	Thermal analysis	727:742	Thermal analysis	727:742	Thermal analysis shows that the processing window of PVA composite is significantly broadened to 131.46 °C.					
34097954	2	44	theme	hydrogen	346:353	arg1	network					363:369	hydrogen bonding network	346:369	hydrogen bonding network	346:369	Based on the regulation and control of hydrogen bonding network, CS and CNFs can extend the processing window and improve the thermoplasticity of PVA composites.					
34097954	3	45	theme	hydrogen	651:658	arg1	bonds					660:664	new hydrogen bonds	647:664	new hydrogen bonds among PVA, CS and CNFs	647:687	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
34097954	4	46	theme	composite	784:792	arg1	window					770:775	the processing window	755:775	the processing window of PVA composite	755:792	Thermal analysis shows that the processing window of PVA composite is significantly broadened to 131.46 °C.					
34097954	2	47	theme	bonding	355:361	arg1	network					363:369	hydrogen bonding network	346:369	hydrogen bonding network	346:369	Based on the regulation and control of hydrogen bonding network, CS and CNFs can extend the processing window and improve the thermoplasticity of PVA composites.					
34097954	4	48	theme	PVA	780:782	arg1	composite					784:792	PVA composite	780:792	PVA composite	780:792	Thermal analysis shows that the processing window of PVA composite is significantly broadened to 131.46 °C.					
34097954	3	49	theme	Raman	513:517	arg1	spectra					519:525	Raman spectra	513:525	Raman spectra	513:525	Fourier transform infrared spectroscopy and Raman spectra analysis indicate that the intra- and inter-molecular hydrogen bonds of PVA are broken, accompanied by the formation of new hydrogen bonds among PVA, CS and CNFs during the melt-processing treatment.					
33295902	3	0	theme	Pinus	333:337	arg1	PPPS					373:376	PPPS	373:376	PPPS	373:376	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	3	0	theme	Pinus	333:337	arg1	polysaccharide					357:370	Pinus massoniana pollen polysaccharide	333:370	Pinus massoniana pollen polysaccharide (PPPS)	333:377	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	8	1	theme	systemic	1106:1113	arg1	immunity					1115:1122	systemic immunity	1106:1122	systemic immunity	1106:1122	Moreover, PPPS also regulated systemic immunity by acting on intestinal PPs.					
33295902	5	2	from	effect	730:735	arg1	microbiota					752:761	gut microbiota	748:761	gut microbiota in mice	748:769	In this work, we initiated our investigation by using 16S rRNA high-throughput sequencing technology to assess the effect of PPPS on gut microbiota in mice.					
33295902	3	3	theme	massoniana	339:348	arg1	PPPS					373:376	PPPS	373:376	PPPS	373:376	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	3	3	theme	massoniana	339:348	arg1	polysaccharide					357:370	Pinus massoniana pollen polysaccharide	333:370	Pinus massoniana pollen polysaccharide (PPPS)	333:377	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	4	4	from	role	525:528	arg1	microenvironment					556:571	the intestinal microenvironment	541:571	the intestinal microenvironment	541:571	However, the role of PPPS in the intestinal microenvironment and intestinal diseases is still unknown.					
33295902	4	4	from	role	525:528	arg1	diseases					588:595	intestinal diseases	577:595	intestinal diseases	577:595	However, the role of PPPS in the intestinal microenvironment and intestinal diseases is still unknown.					
33295902	10	5	from	symptoms	1409:1416	arg1	mice					1466:1469	dextran sodium sulfate (DSS)-induced colitis mice	1421:1469	dextran sodium sulfate (DSS)-induced colitis mice	1421:1469	In addition, PPPS alleviated colonic injury and relieved colitis symptoms in dextran sodium sulfate (DSS)-induced colitis mice.					
33295902	5	6	theme	PPPS	740:743	arg1	effect					730:735	the effect	726:735	the effect of PPPS on gut microbiota in mice	726:769	In this work, we initiated our investigation by using 16S rRNA high-throughput sequencing technology to assess the effect of PPPS on gut microbiota in mice.					
33295902	0	7	from	Effects	0:6	arg1	colitis					86:92	colitis	86:92	colitis	86:92	Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice.					
33295902	0	7	from	Effects	0:6	arg1	microenvironment					65:80	intestinal microenvironment	54:80	intestinal microenvironment	54:80	Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice.					
33295902	5	8	theme	16S	669:671	arg1	rRNA					673:676	16S rRNA	669:676	16S rRNA high-throughput sequencing technology	669:714	In this work, we initiated our investigation by using 16S rRNA high-throughput sequencing technology to assess the effect of PPPS on gut microbiota in mice.					
33295902	11	9	theme	practical	1521:1529	arg1	effect					1542:1547	a practical regulatory effect	1519:1547	a practical regulatory effect	1519:1547	Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.					
33295902	5	10	from	microbiota	752:761	arg1	mice					766:769	mice	766:769	mice	766:769	In this work, we initiated our investigation by using 16S rRNA high-throughput sequencing technology to assess the effect of PPPS on gut microbiota in mice.					
33295902	11	11	theme	potential	1653:1661	arg1	value					1675:1679	the potential application value	1649:1679	the potential application value of PPPS	1649:1687	Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.					
33295902	5	12	theme	rRNA	673:676	arg1	technology					705:714	16S rRNA high-throughput sequencing technology	669:714	16S rRNA high-throughput sequencing technology	669:714	In this work, we initiated our investigation by using 16S rRNA high-throughput sequencing technology to assess the effect of PPPS on gut microbiota in mice.					
33295902	10	13	theme	colonic	1373:1379	arg1	injury					1381:1386	colonic injury	1373:1386	colonic injury	1373:1386	In addition, PPPS alleviated colonic injury and relieved colitis symptoms in dextran sodium sulfate (DSS)-induced colitis mice.					
33295902	2	14	theme	chronic	289:295	arg1	inflammation					297:308	chronic inflammation	289:308	chronic inflammation	289:308	On the contrary, disordered dynamic processes lead to chronic inflammation and disease pathology.					
33295902	5	15	theme	gut	748:750	arg1	microbiota					752:761	gut microbiota	748:761	gut microbiota in mice	748:769	In this work, we initiated our investigation by using 16S rRNA high-throughput sequencing technology to assess the effect of PPPS on gut microbiota in mice.					
33295902	11	16	theme	application	1663:1673	arg1	value					1675:1679	the potential application value	1649:1679	the potential application value of PPPS	1649:1687	Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.					
33295902	10	17	theme	colitis	1458:1464	arg1	mice					1466:1469	dextran sodium sulfate (DSS)-induced colitis mice	1421:1469	dextran sodium sulfate (DSS)-induced colitis mice	1421:1469	In addition, PPPS alleviated colonic injury and relieved colitis symptoms in dextran sodium sulfate (DSS)-induced colitis mice.					
33295902	9	18	theme	-induced	1194:1201	arg1	damage					1214:1219	lipopolysaccharide (LPS) -induced Caco2 cell damage	1169:1219	lipopolysaccharide (LPS) -induced Caco2 cell damage	1169:1219	PPPS alleviated lipopolysaccharide (LPS) -induced Caco2 cell damage, indicating that PPPS has the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells.					
33295902	6	19	theme	probiotics	884:893	arg1	proportion					870:879	the proportion	866:879	the proportion of probiotics	866:893	The result showed that PPPS regulated the composition of gut microbiota in mice and increased the proportion of probiotics.					
33295902	10	20	theme	-induced	1449:1456	arg1	mice					1466:1469	dextran sodium sulfate (DSS)-induced colitis mice	1421:1469	dextran sodium sulfate (DSS)-induced colitis mice	1421:1469	In addition, PPPS alleviated colonic injury and relieved colitis symptoms in dextran sodium sulfate (DSS)-induced colitis mice.					
33295902	9	21	theme	Caco2	1203:1207	arg1	damage					1214:1219	lipopolysaccharide (LPS) -induced Caco2 cell damage	1169:1219	lipopolysaccharide (LPS) -induced Caco2 cell damage	1169:1219	PPPS alleviated lipopolysaccharide (LPS) -induced Caco2 cell damage, indicating that PPPS has the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells.					
33295902	3	22	theme	extensive	450:458	arg1	activities					471:480	extensive biological activities	450:480	extensive biological activities	450:480	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	3	22	theme	extensive	450:458	arg1	regulation					500:509	immune regulation	493:509	immune regulation	493:509	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	8	23	theme	intestinal	1137:1146	arg1	PPs					1148:1150	intestinal PPs	1137:1150	intestinal PPs	1137:1150	Moreover, PPPS also regulated systemic immunity by acting on intestinal PPs.					
33295902	9	24	theme	cell	1209:1212	arg1	damage					1214:1219	lipopolysaccharide (LPS) -induced Caco2 cell damage	1169:1219	lipopolysaccharide (LPS) -induced Caco2 cell damage	1169:1219	PPPS alleviated lipopolysaccharide (LPS) -induced Caco2 cell damage, indicating that PPPS has the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells.					
33295902	1	25	theme	normal	185:190	arg1	activities					206:215	the normal physiological activities	181:215	the normal physiological activities of the intestine	181:232	The stability of the intestinal microenvironment is the basis for maintaining the normal physiological activities of the intestine.					
33295902	9	26	from	dysfunction	1316:1326	arg1	cells					1337:1341	Caco2 cells	1331:1341	Caco2 cells	1331:1341	PPPS alleviated lipopolysaccharide (LPS) -induced Caco2 cell damage, indicating that PPPS has the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells.					
33295902	6	27	theme	gut	829:831	arg1	microbiota					833:842	gut microbiota	829:842	gut microbiota	829:842	The result showed that PPPS regulated the composition of gut microbiota in mice and increased the proportion of probiotics.					
33295902	10	28	theme	colitis	1401:1407	arg1	symptoms					1409:1416	colitis symptoms	1401:1416	colitis symptoms in dextran sodium sulfate (DSS)-induced colitis mice	1401:1469	In addition, PPPS alleviated colonic injury and relieved colitis symptoms in dextran sodium sulfate (DSS)-induced colitis mice.					
33295902	1	29	theme	physiological	192:204	arg1	activities					206:215	the normal physiological activities	181:215	the normal physiological activities of the intestine	181:232	The stability of the intestinal microenvironment is the basis for maintaining the normal physiological activities of the intestine.					
33295902	0	30	theme	massoniana	17:26	arg1	polysaccharides					35:49	Pinus massoniana pollen polysaccharides	11:49	Pinus massoniana pollen polysaccharides	11:49	Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice.					
33295902	9	31	theme	Caco2	1331:1335	arg1	cells					1337:1341	Caco2 cells	1331:1341	Caco2 cells	1331:1341	PPPS alleviated lipopolysaccharide (LPS) -induced Caco2 cell damage, indicating that PPPS has the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells.					
33295902	2	32	theme	disease	314:320	arg1	pathology					322:330	disease pathology	314:330	disease pathology	314:330	On the contrary, disordered dynamic processes lead to chronic inflammation and disease pathology.					
33295902	0	33	theme	Pinus	11:15	arg1	polysaccharides					35:49	Pinus massoniana pollen polysaccharides	11:49	Pinus massoniana pollen polysaccharides	11:49	Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice.					
33295902	11	34	theme	intestinal	1556:1565	arg1	microenvironment					1567:1582	the intestinal microenvironment	1552:1582	the intestinal microenvironment	1552:1582	Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.					
33295902	11	35	theme	PPPS	1684:1687	arg1	value					1675:1679	the potential application value	1649:1679	the potential application value of PPPS	1649:1687	Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.					
33295902	0	36	theme	polysaccharides	35:49	arg1	Effects					0:6	Effects	0:6	Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice.	0:101	Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice.					
33295902	4	37	theme	intestinal	577:586	arg1	diseases					588:595	intestinal diseases	577:595	intestinal diseases	577:595	However, the role of PPPS in the intestinal microenvironment and intestinal diseases is still unknown.					
33295902	0	38	from	colitis	86:92	arg1	mice					97:100	mice	97:100	mice	97:100	Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice.					
33295902	6	39	from	composition	814:824	arg1	mice					847:850	mice	847:850	mice	847:850	The result showed that PPPS regulated the composition of gut microbiota in mice and increased the proportion of probiotics.					
33295902	3	40	theme	biological	460:469	arg1	activities					471:480	extensive biological activities	450:480	extensive biological activities	450:480	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	3	40	theme	biological	460:469	arg1	regulation					500:509	immune regulation	493:509	immune regulation	493:509	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	0	41	theme	pollen	28:33	arg1	polysaccharides					35:49	Pinus massoniana pollen polysaccharides	11:49	Pinus massoniana pollen polysaccharides	11:49	Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice.					
33295902	11	42	theme	regulatory	1531:1540	arg1	effect					1542:1547	a practical regulatory effect	1519:1547	a practical regulatory effect	1519:1547	Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.					
33295902	1	43	theme	intestine	224:232	arg1	activities					206:215	the normal physiological activities	181:215	the normal physiological activities of the intestine	181:232	The stability of the intestinal microenvironment is the basis for maintaining the normal physiological activities of the intestine.					
33295902	0	44	theme	intestinal	54:63	arg1	microenvironment					65:80	intestinal microenvironment	54:80	intestinal microenvironment	54:80	Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice.					
33295902	4	45	theme	PPPS	533:536	arg1	unknown					606:612	unknown	606:612	unknown	606:612	However, the role of PPPS in the intestinal microenvironment and intestinal diseases is still unknown.					
33295902	4	45	theme	PPPS	533:536	arg1	role					525:528	the role	521:528	the role of PPPS in the intestinal microenvironment and intestinal diseases	521:595	However, the role of PPPS in the intestinal microenvironment and intestinal diseases is still unknown.					
33295902	5	46	theme	high-throughput	678:692	arg1	technology					705:714	16S rRNA high-throughput sequencing technology	669:714	16S rRNA high-throughput sequencing technology	669:714	In this work, we initiated our investigation by using 16S rRNA high-throughput sequencing technology to assess the effect of PPPS on gut microbiota in mice.					
33295902	0	47	from	microenvironment	65:80	arg1	mice					97:100	mice	97:100	mice	97:100	Effects of Pinus massoniana pollen polysaccharides on intestinal microenvironment and colitis in mice.					
33295902	7	48	theme	lymphocytes	1038:1048	arg1	state					1029:1033	the immunosuppressive state	1007:1033	the immunosuppressive state of lymphocytes in Peyer's patches (PPs)	1007:1073	Subsequently, we established immunosuppressive mice using cyclophosphamide (CTX) and found that PPPS regulated the immunosuppressive state of lymphocytes in Peyer's patches (PPs).					
33295902	4	49	theme	intestinal	545:554	arg1	microenvironment					556:571	the intestinal microenvironment	541:571	the intestinal microenvironment	541:571	However, the role of PPPS in the intestinal microenvironment and intestinal diseases is still unknown.					
33295902	5	50	theme	sequencing	694:703	arg1	technology					705:714	16S rRNA high-throughput sequencing technology	669:714	16S rRNA high-throughput sequencing technology	669:714	In this work, we initiated our investigation by using 16S rRNA high-throughput sequencing technology to assess the effect of PPPS on gut microbiota in mice.					
33295902	7	51	theme	immunosuppressive	1011:1027	arg1	state					1029:1033	the immunosuppressive state	1007:1033	the immunosuppressive state of lymphocytes in Peyer's patches (PPs)	1007:1073	Subsequently, we established immunosuppressive mice using cyclophosphamide (CTX) and found that PPPS regulated the immunosuppressive state of lymphocytes in Peyer's patches (PPs).					
33295902	11	52	from	effect	1542:1547	arg1	microenvironment					1567:1582	the intestinal microenvironment	1552:1582	the intestinal microenvironment	1552:1582	Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.					
33295902	9	53	contain	has	1243:1245	arg1	PPPS					1238:1241	PPPS	1238:1241	PPPS	1238:1241	PPPS alleviated lipopolysaccharide (LPS) -induced Caco2 cell damage, indicating that PPPS has the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells.					
33295902	9	53	contain	has	1243:1245	arg2	ability					1251:1257	the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells	1247:1341	the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells	1247:1341	PPPS alleviated lipopolysaccharide (LPS) -induced Caco2 cell damage, indicating that PPPS has the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells.					
33295902	9	54	theme	barrier	1308:1314	arg1	dysfunction					1316:1326	the barrier dysfunction	1304:1326	the barrier dysfunction in Caco2 cells	1304:1341	PPPS alleviated lipopolysaccharide (LPS) -induced Caco2 cell damage, indicating that PPPS has the ability to reduce the damage and effectively improve the barrier dysfunction in Caco2 cells.					
33295902	1	55	theme	intestinal	124:133	arg1	microenvironment					135:150	the intestinal microenvironment	120:150	the intestinal microenvironment	120:150	The stability of the intestinal microenvironment is the basis for maintaining the normal physiological activities of the intestine.					
33295902	7	56	theme	immunosuppressive	925:941	arg1	mice					943:946	immunosuppressive mice	925:946	immunosuppressive mice using cyclophosphamide (CTX)	925:975	Subsequently, we established immunosuppressive mice using cyclophosphamide (CTX) and found that PPPS regulated the immunosuppressive state of lymphocytes in Peyer's patches (PPs).					
33295902	6	57	theme	microbiota	833:842	arg1	composition					814:824	the composition	810:824	the composition of gut microbiota in mice	810:850	The result showed that PPPS regulated the composition of gut microbiota in mice and increased the proportion of probiotics.					
33295902	2	58	theme	dynamic	263:269	arg1	processes					271:279	disordered dynamic processes	252:279	disordered dynamic processes	252:279	On the contrary, disordered dynamic processes lead to chronic inflammation and disease pathology.					
33295902	11	59	theme	essential	1603:1611	arg1	basis					1625:1629	an essential theoretical basis	1600:1629	an essential theoretical basis for us to develop the potential application value of PPPS further	1600:1695	Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.					
33295902	1	60	theme	microenvironment	135:150	arg1	basis					159:163	the basis	155:163	the basis for maintaining the normal physiological activities of the intestine	155:232	The stability of the intestinal microenvironment is the basis for maintaining the normal physiological activities of the intestine.					
33295902	1	60	theme	microenvironment	135:150	arg1	stability					107:115	The stability	103:115	The stability of the intestinal microenvironment	103:150	The stability of the intestinal microenvironment is the basis for maintaining the normal physiological activities of the intestine.					
33295902	3	61	theme	immune	493:498	arg1	regulation					500:509	immune regulation	493:509	immune regulation	493:509	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	3	62	theme	pollen	350:355	arg1	PPPS					373:376	PPPS	373:376	PPPS	373:376	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	3	62	theme	pollen	350:355	arg1	polysaccharide					357:370	Pinus massoniana pollen polysaccharide	333:370	Pinus massoniana pollen polysaccharide (PPPS)	333:377	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	3	63	attach	isolated	380:387	arg2	PPPS					373:376	PPPS	373:376	PPPS	373:376	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	3	63	attach	isolated	380:387	arg2	polysaccharide					357:370	Pinus massoniana pollen polysaccharide	333:370	Pinus massoniana pollen polysaccharide (PPPS)	333:377	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	3	63	attach	isolated	380:387	arg1	massoniana					408:417	massoniana	408:417	massoniana	408:417	Pinus massoniana pollen polysaccharide (PPPS), isolated from Taishan Pinus massoniana pollen, has been reported with extensive biological activities, including immune regulation.					
33295902	2	64	theme	disordered	252:261	arg1	processes					271:279	disordered dynamic processes	252:279	disordered dynamic processes	252:279	On the contrary, disordered dynamic processes lead to chronic inflammation and disease pathology.					
33295902	11	65	theme	theoretical	1613:1623	arg1	basis					1625:1629	an essential theoretical basis	1600:1629	an essential theoretical basis for us to develop the potential application value of PPPS further	1600:1695	Overall, our findings indicate that PPPS shows a practical regulatory effect in the intestinal microenvironment, which provides an essential theoretical basis for us to develop the potential application value of PPPS further.					
32172834	1	0	theme	discharge	321:329	arg1	conditions					286:295	different conditions	276:295	different conditions (40 W and 80 W power of discharge, const	276:336	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	0	theme	discharge	321:329	arg1	40 W					298:301	40 W	298:301	40 W	298:301	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	0	theme	discharge	321:329	arg1	power					312:316	80 W power	307:316	80 W power	307:316	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	5	1	theme	chemical	1078:1085	arg1	treatment					1087:1095	the chemical treatment	1074:1095	the chemical treatment	1074:1095	This investigation shows that plasma can be successfully used for improvement of raw hemp fibers wettability, even to substitute the chemical treatment for some applications of hemp.					
32172834	2	2	theme	time	339:342	arg1	120 s					344:348	time 120 s)	339:349	time 120 s) in order	339:358	time 120 s) in order to study influence of plasma treatment on their structure and sorption properties.					
32172834	1	3	theme	pressure	232:239	arg1	discharge					260:268	atmospheric pressure dielectric barrier discharge	220:268	atmospheric pressure dielectric barrier discharge	220:268	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	0	4	theme	sorption	66:73	arg1	properties					75:84	sorption properties	66:84	sorption properties	66:84	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	3	5	theme	surface	658:664	arg1	etching					666:672	intensive surface etching	648:672	intensive surface etching	648:672	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	1	6	theme	Hemp	139:142	arg1	fibers					144:149	Hemp fibers	139:149	Hemp fibers with different amount of hemicelluloses and lignin	139:200	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	7	theme	dielectric	241:250	arg1	discharge					260:268	atmospheric pressure dielectric barrier discharge	220:268	atmospheric pressure dielectric barrier discharge	220:268	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	0	8	theme	lignocellulosic	89:103	arg1	L					134:134	Cannabis sativa L.	118:135	Cannabis sativa L.	118:135	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	0	8	theme	lignocellulosic	89:103	arg1	fibers					110:115	lignocellulosic hemp fibers	89:115	lignocellulosic hemp fibers (Cannabis sativa L.)	89:136	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	0	9	from	Effect	0:5	arg1	structure					52:60	structure	52:60	structure	52:60	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	0	9	from	Effect	0:5	arg1	properties					75:84	sorption properties	66:84	sorption properties	66:84	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	0	9	from	Effect	0:5	arg1	composition					39:49	chemical composition	30:49	chemical composition	30:49	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	1	10	theme	barrier	252:258	arg1	discharge					260:268	atmospheric pressure dielectric barrier discharge	220:268	atmospheric pressure dielectric barrier discharge	220:268	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	4	11	theme	lower	876:880	arg1	content					882:888	lower content	876:888	lower content of lignin	876:898	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	0	12	theme	fibers	110:115	arg1	structure					52:60	structure	52:60	structure	52:60	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	0	12	theme	fibers	110:115	arg1	properties					75:84	sorption properties	66:84	sorption properties	66:84	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	0	12	theme	fibers	110:115	arg1	composition					39:49	chemical composition	30:49	chemical composition	30:49	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	4	13	theme	plasma	698:703	arg1	treatment					705:713	plasma treatment	698:713	plasma treatment	698:713	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	2	14	theme	plasma	382:387	arg1	treatment					389:397	plasma treatment	382:397	plasma treatment	382:397	time 120 s) in order to study influence of plasma treatment on their structure and sorption properties.					
32172834	0	15	theme	hemp	105:108	arg1	L					134:134	Cannabis sativa L.	118:135	Cannabis sativa L.	118:135	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	0	15	theme	hemp	105:108	arg1	fibers					110:115	lignocellulosic hemp fibers	89:115	lignocellulosic hemp fibers (Cannabis sativa L.)	89:136	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	1	16	dep	40 W	298:301	arg1	const					332:336	const	332:336	const	332:336	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	4	17	theme	lower	765:769	arg1	content					771:777	lower content	765:777	lower content of hemicelluloses (increase up to 9 times)	765:820	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	4	18	theme	lignin	893:898	arg1	content					882:888	lower content	876:888	lower content of lignin	876:898	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	4	19	dep	9	813:813	arg1	to					810:811	to	810:811	to	810:811	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	5	20	theme	fibers	1035:1040	arg1	wettability					1042:1052	raw hemp fibers wettability	1026:1052	raw hemp fibers wettability	1026:1052	This investigation shows that plasma can be successfully used for improvement of raw hemp fibers wettability, even to substitute the chemical treatment for some applications of hemp.					
32172834	4	21	dep	increase	798:805	arg1	times					815:819	up to 9 times	807:819	increase up to 9 times	798:819	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	5	22	theme	raw	1026:1028	arg1	fibers					1035:1040	raw hemp fibers	1026:1040	raw hemp fibers wettability	1026:1052	This investigation shows that plasma can be successfully used for improvement of raw hemp fibers wettability, even to substitute the chemical treatment for some applications of hemp.					
32172834	1	23	theme	different	156:164	arg1	hemicelluloses					176:189	hemicelluloses	176:189	hemicelluloses	176:189	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	23	theme	different	156:164	arg1	amount					166:171	different amount	156:171	different amount of hemicelluloses and lignin	156:200	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	23	theme	different	156:164	arg1	lignin					195:200	lignin	195:200	lignin	195:200	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	5	24	theme	hemp	1122:1125	arg1	applications					1106:1117	some applications	1101:1117	some applications of hemp	1101:1125	This investigation shows that plasma can be successfully used for improvement of raw hemp fibers wettability, even to substitute the chemical treatment for some applications of hemp.					
32172834	3	25	theme	hemp	540:543	arg1	chemistry					559:567	hemp fiber surface chemistry	540:567	hemp fiber surface chemistry	540:567	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	0	26	theme	treatment	17:25	arg1	Effect					0:5	Effect	0:5	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).	0:137	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	1	27	theme	different	276:284	arg1	conditions					286:295	different conditions	276:295	different conditions (40 W and 80 W power of discharge, const	276:336	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	27	theme	different	276:284	arg1	40 W					298:301	40 W	298:301	40 W	298:301	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	27	theme	different	276:284	arg1	power					312:316	80 W power	307:316	80 W power	307:316	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	0	28	theme	Cannabis	118:125	arg1	L					134:134	Cannabis sativa L.	118:135	Cannabis sativa L.	118:135	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	0	28	theme	Cannabis	118:125	arg1	fibers					110:115	lignocellulosic hemp fibers	89:115	lignocellulosic hemp fibers (Cannabis sativa L.)	89:136	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	4	29	theme	hemicelluloses	782:795	arg1	content					771:777	lower content	765:777	lower content of hemicelluloses (increase up to 9 times)	765:820	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	2	30	theme	treatment	389:397	arg1	influence					369:377	influence	369:377	influence of plasma treatment on their structure and sorption properties	369:440	time 120 s) in order to study influence of plasma treatment on their structure and sorption properties.					
32172834	0	31	theme	plasma	10:15	arg1	treatment					17:25	plasma treatment	10:25	plasma treatment	10:25	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	5	32	used	used	1002:1005	arg2	plasma					975:980	plasma	975:980	plasma	975:980	This investigation shows that plasma can be successfully used for improvement of raw hemp fibers wettability, even to substitute the chemical treatment for some applications of hemp.					
32172834	4	33	theme	fibers	853:858	arg1	wettability					829:839	wettability	829:839	wettability of raw hemp fibers and fibers with lower content of lignin	829:898	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	4	34	theme	fibers	864:869	arg1	wettability					829:839	wettability	829:839	wettability of raw hemp fibers and fibers with lower content of lignin	829:898	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	5	35	theme	hemp	1030:1033	arg1	fibers					1035:1040	raw hemp fibers	1026:1040	raw hemp fibers wettability	1026:1052	This investigation shows that plasma can be successfully used for improvement of raw hemp fibers wettability, even to substitute the chemical treatment for some applications of hemp.					
32172834	1	36	theme	hemicelluloses	176:189	arg1	hemicelluloses					176:189	hemicelluloses	176:189	hemicelluloses	176:189	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	36	theme	hemicelluloses	176:189	arg1	amount					166:171	different amount	156:171	different amount of hemicelluloses and lignin	156:200	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	36	theme	hemicelluloses	176:189	arg1	lignin					195:200	lignin	195:200	lignin	195:200	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	0	37	theme	chemical	30:37	arg1	composition					39:49	chemical composition	30:49	chemical composition	30:49	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	3	38	dep	increased	508:516	arg1	confirmed					569:577	confirmed	569:577	increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM	508:689	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	4	39	with	wettability	829:839	arg1	content					882:888	lower content	876:888	lower content of lignin	876:898	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	0	40	theme	sativa	127:132	arg1	L					134:134	Cannabis sativa L.	118:135	Cannabis sativa L.	118:135	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	0	40	theme	sativa	127:132	arg1	fibers					110:115	lignocellulosic hemp fibers	89:115	lignocellulosic hemp fibers (Cannabis sativa L.)	89:136	Effect of plasma treatment on chemical composition, structure and sorption properties of lignocellulosic hemp fibers (Cannabis sativa L.).					
32172834	1	41	theme	lignin	195:200	arg1	hemicelluloses					176:189	hemicelluloses	176:189	hemicelluloses	176:189	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	41	theme	lignin	195:200	arg1	amount					166:171	different amount	156:171	different amount of hemicelluloses and lignin	156:200	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	41	theme	lignin	195:200	arg1	lignin					195:200	lignin	195:200	lignin	195:200	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	3	42	theme	increased	608:616	arg1	roughness					618:626	increased roughness	608:626	increased roughness	608:626	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	3	43	theme	fiber	545:549	arg1	chemistry					559:567	hemp fiber surface chemistry	540:567	hemp fiber surface chemistry	540:567	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	4	44	theme	hemp	848:851	arg1	fibers					853:858	raw hemp fibers	844:858	raw hemp fibers	844:858	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	3	45	theme	treated	465:471	arg1	samples					473:479	plasma treated samples	458:479	plasma treated samples	458:479	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	2	46	theme	sorption	422:429	arg1	properties					431:440	sorption properties	422:440	sorption properties	422:440	time 120 s) in order to study influence of plasma treatment on their structure and sorption properties.					
32172834	1	47	theme	80 W	307:310	arg1	conditions					286:295	different conditions	276:295	different conditions (40 W and 80 W power of discharge, const	276:336	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	47	theme	80 W	307:310	arg1	power					312:316	80 W power	307:316	80 W power	307:316	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	5	48	theme	wettability	1042:1052	arg1	improvement					1011:1021	improvement	1011:1021	improvement of raw hemp fibers wettability	1011:1052	This investigation shows that plasma can be successfully used for improvement of raw hemp fibers wettability, even to substitute the chemical treatment for some applications of hemp.					
32172834	4	49	dep	hemicelluloses	782:795	arg1	increase					798:805	increase	798:805	increase up to 9 times	798:819	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	3	50	theme	surface	551:557	arg1	chemistry					559:567	hemp fiber surface chemistry	540:567	hemp fiber surface chemistry	540:567	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	3	51	theme	samples	473:479	arg1	Wettability					443:453	Wettability	443:453	Wettability of plasma treated samples	443:479	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	1	52	with	fibers	144:149	arg1	hemicelluloses					176:189	hemicelluloses	176:189	hemicelluloses	176:189	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	52	with	fibers	144:149	arg1	amount					166:171	different amount	156:171	different amount of hemicelluloses and lignin	156:200	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	52	with	fibers	144:149	arg1	lignin					195:200	lignin	195:200	lignin	195:200	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	3	53	theme	intensive	648:656	arg1	etching					666:672	intensive surface etching	648:672	intensive surface etching	648:672	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	3	54	theme	FTIR	586:589	arg1	spectroscopy					591:602	ATR FTIR spectroscopy	582:602	ATR FTIR spectroscopy	582:602	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	3	55	from	changes	529:535	arg1	chemistry					559:567	hemp fiber surface chemistry	540:567	hemp fiber surface chemistry	540:567	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	4	56	theme	raw	844:846	arg1	fibers					853:858	raw hemp fibers	844:858	raw hemp fibers	844:858	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	4	57	theme	hemp	748:751	arg1	fibers					753:758	hemp fibers	748:758	hemp fibers with lower content of hemicelluloses (increase up to 9 times)	748:820	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	3	58	theme	plasma	458:463	arg1	samples					473:479	plasma treated samples	458:479	plasma treated samples	458:479	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	3	59	theme	etching	666:672	arg1	consequence					633:643	a consequence	631:643	a consequence of intensive surface etching, observed by SEM	631:689	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	2	60	from	influence	369:377	arg1	structure					408:416	their structure	402:416	their structure	402:416	time 120 s) in order to study influence of plasma treatment on their structure and sorption properties.					
32172834	2	60	from	influence	369:377	arg1	properties					431:440	sorption properties	422:440	sorption properties	422:440	time 120 s) in order to study influence of plasma treatment on their structure and sorption properties.					
32172834	4	61	with	fibers	753:758	arg1	content					771:777	lower content	765:777	lower content of hemicelluloses (increase up to 9 times)	765:820	After plasma treatment, wettability was the highest for hemp fibers with lower content of hemicelluloses (increase up to 9 times), while wettability of raw hemp fibers and fibers with lower content of lignin increased about 5 and 2 times, respectively.					
32172834	1	62	dep	conditions	286:295	arg1	conditions					286:295	different conditions	276:295	different conditions (40 W and 80 W power of discharge, const	276:336	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	62	dep	conditions	286:295	arg1	40 W					298:301	40 W	298:301	40 W	298:301	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	1	62	dep	conditions	286:295	arg1	power					312:316	80 W power	307:316	80 W power	307:316	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
32172834	3	63	theme	ATR	582:584	arg1	spectroscopy					591:602	ATR FTIR spectroscopy	582:602	ATR FTIR spectroscopy	582:602	Wettability of plasma treated samples, compared with precursors, increased due to the changes in hemp fiber surface chemistry confirmed by ATR FTIR spectroscopy and increased roughness as a consequence of intensive surface etching, observed by SEM.					
32172834	1	64	theme	atmospheric	220:230	arg1	discharge					260:268	atmospheric pressure dielectric barrier discharge	220:268	atmospheric pressure dielectric barrier discharge	220:268	Hemp fibers with different amount of hemicelluloses and lignin were subjected to atmospheric pressure dielectric barrier discharge under different conditions (40 W and 80 W power of discharge, const.					
33911150	0	0	theme	sustained	91:99	arg1	system					115:120	sustained drug delivery system	91:120	sustained drug delivery system	91:120	A double-layer hydrogel based on alginate-carboxymethyl cellulose and synthetic polymer as sustained drug delivery system.					
33911150	5	1	theme	model	837:841	arg1	drugs					843:847	different model drugs	827:847	different model drugs	827:847	The drug-release results demonstrated that the sustained-release effect of the hydrogels for different model drugs could be regulated by changing the composition or thickness of the hydrogel layer.					
33911150	5	2	dep	composition	884:894	arg1	the					880:882	the	880:882	the	880:882	The drug-release results demonstrated that the sustained-release effect of the hydrogels for different model drugs could be regulated by changing the composition or thickness of the hydrogel layer.					
33911150	6	3	theme	sustained-release	948:964	arg1	effect					966:971	The significant sustained-release effect	932:971	The significant sustained-release effect for BSA and indomethacin	932:996	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	6	4	theme	novel	1058:1062	arg1	system					1083:1088	a novel sustained delivery system	1056:1088	a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods	1056:1179	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	1	5	theme	different	276:284	arg1	hydrogels					298:306	different inner/outer hydrogels	276:306	different inner/outer hydrogels	276:306	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	1	6	theme	hydrogel	167:174	arg1	system					194:199	A new double-layer, pH-sensitive, composite hydrogel sustained-release system	123:199	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels	123:306	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	1	7	theme	inner/outer	286:296	arg1	hydrogels					298:306	different inner/outer hydrogels	276:306	different inner/outer hydrogels	276:306	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	0	8	theme	delivery	106:113	arg1	system					115:120	sustained drug delivery system	91:120	sustained drug delivery system	91:120	A double-layer hydrogel based on alginate-carboxymethyl cellulose and synthetic polymer as sustained drug delivery system.					
33911150	2	9	theme	polysaccharides	326:340	arg1	core					348:351	The polysaccharides inner core	322:351	The polysaccharides inner core	322:351	The polysaccharides inner core based on sodium alginate (SA) and carboxymethyl cellulose (CMC), was formed by physical crosslinking with pH-sensitive property.					
33911150	1	10	theme	hydrogels	298:306	arg1	functions					263:271	combined functions	254:271	combined functions of different inner/outer hydrogels	254:306	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	0	11	theme	drug	101:104	arg1	system					115:120	sustained drug delivery system	91:120	sustained drug delivery system	91:120	A double-layer hydrogel based on alginate-carboxymethyl cellulose and synthetic polymer as sustained drug delivery system.					
33911150	5	12	theme	layer	925:929	arg1	thickness					899:907	thickness	899:907	thickness	899:907	The drug-release results demonstrated that the sustained-release effect of the hydrogels for different model drugs could be regulated by changing the composition or thickness of the hydrogel layer.					
33911150	5	12	theme	layer	925:929	arg1	composition					884:894	composition	884:894	composition	884:894	The drug-release results demonstrated that the sustained-release effect of the hydrogels for different model drugs could be regulated by changing the composition or thickness of the hydrogel layer.					
33911150	3	13	with	out-layer	504:512	arg1	stability					528:536	enhanced stability	519:536	enhanced stability	519:536	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	4	14	theme	physicochemical	659:673	arg1	structure					675:683	The physicochemical structure	655:683	The physicochemical structure of the double-layer hydrogels	655:713	The physicochemical structure of the double-layer hydrogels was characterized.					
33911150	1	15	theme	sustained-release	176:192	arg1	system					194:199	A new double-layer, pH-sensitive, composite hydrogel sustained-release system	123:199	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels	123:306	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	5	16	theme	hydrogels	813:821	arg1	effect					799:804	the sustained-release effect	777:804	the sustained-release effect of the hydrogels for different model drugs	777:847	The drug-release results demonstrated that the sustained-release effect of the hydrogels for different model drugs could be regulated by changing the composition or thickness of the hydrogel layer.					
33911150	4	17	theme	double-layer	692:703	arg1	hydrogels					705:713	the double-layer hydrogels	688:713	the double-layer hydrogels	688:713	The physicochemical structure of the double-layer hydrogels was characterized.					
33911150	0	18	theme	double-layer	2:13	arg1	hydrogel					15:22	A double-layer hydrogel	0:22	A double-layer hydrogel	0:22	A double-layer hydrogel based on alginate-carboxymethyl cellulose and synthetic polymer as sustained drug delivery system.					
33911150	3	19	theme	chemical	556:563	arg1	crosslinking					565:576	chemical crosslinking	556:576	chemical crosslinking	556:576	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	6	20	theme	functional	1164:1173	arg1	foods					1175:1179	functional foods	1164:1179	functional foods	1164:1179	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	3	21	theme	inner	608:612	arg1	core					614:617	inner core	608:617	inner core	608:617	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	2	22	theme	pH-sensitive	459:470	arg1	property					472:479	pH-sensitive property	459:479	pH-sensitive property	459:479	The polysaccharides inner core based on sodium alginate (SA) and carboxymethyl cellulose (CMC), was formed by physical crosslinking with pH-sensitive property.					
33911150	6	23	from	applications	1138:1149	arg1	drugs					1154:1158	drugs	1154:1158	drugs	1154:1158	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	6	23	from	applications	1138:1149	arg1	foods					1175:1179	functional foods	1164:1179	functional foods	1164:1179	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	3	24	theme	core	614:617	arg1	diffusion					627:635	the diffusion	623:635	the diffusion of inner content	623:652	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	3	24	theme	core	614:617	arg1	expansion					595:603	the expansion	591:603	the expansion of inner core	591:617	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	2	25	theme	physical	432:439	arg1	crosslinking					441:452	physical crosslinking	432:452	physical crosslinking with pH-sensitive property	432:479	The polysaccharides inner core based on sodium alginate (SA) and carboxymethyl cellulose (CMC), was formed by physical crosslinking with pH-sensitive property.					
33911150	1	26	with	polymers	240:247	arg1	functions					263:271	combined functions	254:271	combined functions of different inner/outer hydrogels	254:306	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	6	27	theme	significant	936:946	arg1	effect					966:971	The significant sustained-release effect	932:971	The significant sustained-release effect for BSA and indomethacin	932:996	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	2	28	theme	inner	342:346	arg1	core					348:351	The polysaccharides inner core	322:351	The polysaccharides inner core	322:351	The polysaccharides inner core based on sodium alginate (SA) and carboxymethyl cellulose (CMC), was formed by physical crosslinking with pH-sensitive property.					
33911150	3	29	theme	polymer	496:502	arg1	out-layer					504:512	The synthetic polymer out-layer	482:512	The synthetic polymer out-layer with enhanced stability	482:536	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	0	30	theme	alginate-carboxymethyl	33:54	arg1	cellulose					56:64	alginate-carboxymethyl cellulose	33:64	alginate-carboxymethyl cellulose	33:64	A double-layer hydrogel based on alginate-carboxymethyl cellulose and synthetic polymer as sustained drug delivery system.					
33911150	1	31	with	polysaccharides	210:224	arg1	functions					263:271	combined functions	254:271	combined functions of different inner/outer hydrogels	254:306	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	1	32	theme	synthetic	230:238	arg1	polymers					240:247	synthetic polymers	230:247	synthetic polymers	230:247	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	4	33	theme	hydrogels	705:713	arg1	structure					675:683	The physicochemical structure	655:683	The physicochemical structure of the double-layer hydrogels	655:713	The physicochemical structure of the double-layer hydrogels was characterized.					
33911150	5	34	theme	sustained-release	781:797	arg1	effect					799:804	the sustained-release effect	777:804	the sustained-release effect of the hydrogels for different model drugs	777:847	The drug-release results demonstrated that the sustained-release effect of the hydrogels for different model drugs could be regulated by changing the composition or thickness of the hydrogel layer.					
33911150	6	35	theme	delivery	1074:1081	arg1	system					1083:1088	a novel sustained delivery system	1056:1088	a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods	1056:1179	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	6	36	theme	potential	1128:1136	arg1	applications					1138:1149	potential applications	1128:1149	potential applications in drugs and functional foods	1128:1179	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	5	37	theme	hydrogel	916:923	arg1	layer					925:929	the hydrogel layer	912:929	the hydrogel layer	912:929	The drug-release results demonstrated that the sustained-release effect of the hydrogels for different model drugs could be regulated by changing the composition or thickness of the hydrogel layer.					
33911150	5	38	theme	different	827:835	arg1	drugs					843:847	different model drugs	827:847	different model drugs	827:847	The drug-release results demonstrated that the sustained-release effect of the hydrogels for different model drugs could be regulated by changing the composition or thickness of the hydrogel layer.					
33911150	2	39	with	crosslinking	441:452	arg1	property					472:479	pH-sensitive property	459:479	pH-sensitive property	459:479	The polysaccharides inner core based on sodium alginate (SA) and carboxymethyl cellulose (CMC), was formed by physical crosslinking with pH-sensitive property.					
33911150	3	40	theme	enhanced	519:526	arg1	stability					528:536	enhanced stability	519:536	enhanced stability	519:536	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	6	41	theme	bilayer	1017:1023	arg1	hydrogel					1025:1032	the bilayer hydrogel	1013:1032	the bilayer hydrogel	1013:1032	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	6	42	with	substance	1104:1112	arg1	applications					1138:1149	potential applications	1128:1149	potential applications in drugs and functional foods	1128:1179	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	1	43	theme	new	125:127	arg1	system					194:199	A new double-layer, pH-sensitive, composite hydrogel sustained-release system	123:199	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels	123:306	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	5	44	theme	drug-release	738:749	arg1	results					751:757	The drug-release results	734:757	The drug-release results	734:757	The drug-release results demonstrated that the sustained-release effect of the hydrogels for different model drugs could be regulated by changing the composition or thickness of the hydrogel layer.					
33911150	6	45	with	drugs	1117:1121	arg1	applications					1138:1149	potential applications	1128:1149	potential applications in drugs and functional foods	1128:1179	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	1	46	theme	double-layer	129:140	arg1	system					194:199	A new double-layer, pH-sensitive, composite hydrogel sustained-release system	123:199	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels	123:306	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	6	47	theme	sustained	1064:1072	arg1	system					1083:1088	a novel sustained delivery system	1056:1088	a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods	1056:1179	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	1	48	dep	double-layer	129:140	arg1	composite					157:165	composite	157:165	composite	157:165	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	1	48	dep	double-layer	129:140	arg1	pH-sensitive					143:154	pH-sensitive	143:154	pH-sensitive	143:154	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	2	49	theme	sodium	362:367	arg1	SA					379:380	SA	379:380	SA	379:380	The polysaccharides inner core based on sodium alginate (SA) and carboxymethyl cellulose (CMC), was formed by physical crosslinking with pH-sensitive property.					
33911150	2	49	theme	sodium	362:367	arg1	alginate					369:376	sodium alginate	362:376	sodium alginate (SA)	362:381	The polysaccharides inner core based on sodium alginate (SA) and carboxymethyl cellulose (CMC), was formed by physical crosslinking with pH-sensitive property.					
33911150	1	50	theme	combined	254:261	arg1	functions					263:271	combined functions	254:271	combined functions of different inner/outer hydrogels	254:306	A new double-layer, pH-sensitive, composite hydrogel sustained-release system based on polysaccharides and synthetic polymers with combined functions of different inner/outer hydrogels was prepared.					
33911150	3	51	theme	inner	640:644	arg1	content					646:652	inner content	640:652	inner content	640:652	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	3	52	theme	synthetic	486:494	arg1	out-layer					504:512	The synthetic polymer out-layer	482:512	The synthetic polymer out-layer with enhanced stability	482:536	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	6	53	theme	bioactive	1094:1102	arg1	substance					1104:1112	bioactive substance	1094:1112	bioactive substance	1094:1112	The significant sustained-release effect for BSA and indomethacin indicated that the bilayer hydrogel can be developed into a novel sustained delivery system for bioactive substance or drugs with potential applications in drugs and functional foods.					
33911150	0	54	theme	synthetic	70:78	arg1	polymer					80:86	synthetic polymer	70:86	synthetic polymer	70:86	A double-layer hydrogel based on alginate-carboxymethyl cellulose and synthetic polymer as sustained drug delivery system.					
33911150	3	55	theme	content	646:652	arg1	diffusion					627:635	the diffusion	623:635	the diffusion of inner content	623:652	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	3	55	theme	content	646:652	arg1	expansion					595:603	the expansion	591:603	the expansion of inner core	591:617	The synthetic polymer out-layer with enhanced stability was introduced by chemical crosslinking to eliminate the expansion of inner core and the diffusion of inner content.					
33911150	2	56	theme	carboxymethyl	387:399	arg1	CMC					412:414	CMC	412:414	CMC	412:414	The polysaccharides inner core based on sodium alginate (SA) and carboxymethyl cellulose (CMC), was formed by physical crosslinking with pH-sensitive property.					
33911150	2	56	theme	carboxymethyl	387:399	arg1	cellulose					401:409	carboxymethyl cellulose	387:409	carboxymethyl cellulose (CMC)	387:415	The polysaccharides inner core based on sodium alginate (SA) and carboxymethyl cellulose (CMC), was formed by physical crosslinking with pH-sensitive property.					
33232697	1	0	from	impacts	412:418	arg1	microbiota					433:442	human gut microbiota	423:442	human gut microbiota	423:442	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	1	1	theme	in	320:321	arg1	digestion					363:371	in vitro simulated saliva-gastrointestinal digestion	320:371	in vitro simulated saliva-gastrointestinal digestion	320:371	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	6	2	theme	functional	1553:1562	arg1	industry					1569:1576	the functional food industry	1549:1576	the functional food industry	1549:1576	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	0	3	from	microbiota	162:171	arg1	changes					99:105	Dynamic changes	91:105	Dynamic changes in physicochemical properties and impacts on human gut microbiota	91:171	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	1	4	from	properties	258:267	arg1	loquat					293:298	loquat	293:298	loquat	293:298	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	1	4	from	properties	258:267	arg1	LLP					308:310	LLP	308:310	LLP	308:310	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	2	5	theme	sugar	490:494	arg1	contents					469:476	the contents	465:476	the contents of reducing sugar of LLP	465:501	Results showed that the contents of reducing sugar of LLP slightly increased during the gastrointestinal digestion, and its molecular weight also slightly decreased, suggesting that LLP could be slightly degraded under the gastrointestinal digestion conditions.					
33232697	3	6	theme	LLP-I	880:884	arg1	monosaccharides					861:875	constituent monosaccharides	849:875	constituent monosaccharides of LLP-I	849:884	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	6	theme	LLP-I	880:884	arg1	galactose					931:939	galactose	931:939	galactose	931:939	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	6	theme	LLP-I	880:884	arg1	acid					906:909	glucuronic acid	895:909	glucuronic acid	895:909	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	6	theme	LLP-I	880:884	arg1	acid					925:928	galacturonic acid	912:928	galacturonic acid	912:928	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	6	theme	LLP-I	880:884	arg1	arabinose					946:954	arabinose	946:954	arabinose	946:954	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	0	7	theme	Dynamic	91:97	arg1	changes					99:105	Dynamic changes	91:105	Dynamic changes in physicochemical properties and impacts on human gut microbiota	91:171	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	1	8	theme	simulated	329:337	arg1	digestion					363:371	in vitro simulated saliva-gastrointestinal digestion	320:371	in vitro simulated saliva-gastrointestinal digestion	320:371	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	3	9	theme	gut	1042:1044	arg1	microbiota					1046:1055	human gut microbiota	1036:1055	human gut microbiota	1036:1055	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	6	10	from	prebiotic	1536:1544	arg1	industry					1569:1576	the functional food industry	1549:1576	the functional food industry	1549:1576	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	5	11	theme	fatty	1362:1366	arg1	acids					1368:1372	health-promoting short chain fatty acids	1333:1372	health-promoting short chain fatty acids	1333:1372	LLP-I could also promote the production of health-promoting short chain fatty acids.					
33232697	1	12	theme	physicochemical	242:256	arg1	properties					258:267	physicochemical properties	242:267	physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation	242:394	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	1	13	theme	saliva-gastrointestinal	339:361	arg1	digestion					363:371	in vitro simulated saliva-gastrointestinal digestion	320:371	in vitro simulated saliva-gastrointestinal digestion	320:371	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	6	14	dep	in	1436:1437	arg1	vitro					1439:1443	vitro	1439:1443	vitro	1439:1443	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	5	15	theme	short	1350:1354	arg1	acids					1368:1372	health-promoting short chain fatty acids	1333:1372	health-promoting short chain fatty acids	1333:1372	LLP-I could also promote the production of health-promoting short chain fatty acids.					
33232697	1	16	theme	properties	258:267	arg1	impacts					412:418	its related impacts	400:418	its related impacts on human gut microbiota	400:442	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	1	16	theme	properties	258:267	arg1	changes					231:237	the dynamic changes	219:237	the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation	219:394	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	0	17	from	fermentation	39:50	arg1	loquat					76:81	loquat	76:81	loquat	76:81	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	6	18	from	study	1393:1397	arg1	Results					1375:1381	Results	1375:1381	Results from this study	1375:1397	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	3	19	theme	galacturonic	912:923	arg1	acid					925:928	galacturonic acid	912:928	galacturonic acid	912:928	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	2	20	theme	reducing	481:488	arg1	sugar					490:494	reducing sugar	481:494	reducing sugar of LLP	481:501	Results showed that the contents of reducing sugar of LLP slightly increased during the gastrointestinal digestion, and its molecular weight also slightly decreased, suggesting that LLP could be slightly degraded under the gastrointestinal digestion conditions.					
33232697	1	21	from	loquat	293:298	arg1	polysaccharides					272:286	polysaccharides	272:286	polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation	272:394	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	1	21	from	loquat	293:298	arg1	properties					258:267	physicochemical properties	242:267	physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation	242:394	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	1	22	dep	loquat	293:298	arg1	leaves					300:305	leaves	300:305	leaves	300:305	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	6	23	theme	fecal	1459:1463	arg1	behaviors					1478:1486	the in vitro digestion and fecal fermentation behaviors	1432:1486	behaviors	1478:1486	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	1	24	theme	fecal	377:381	arg1	fermentation					383:394	fecal fermentation	377:394	fecal fermentation	377:394	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	3	25	theme	human	1036:1040	arg1	microbiota					1046:1055	human gut microbiota	1036:1055	human gut microbiota	1036:1055	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	0	26	dep	leaves	83:88	arg1	changes					99:105	Dynamic changes	91:105	Dynamic changes in physicochemical properties and impacts on human gut microbiota	91:171	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	5	27	theme	acids	1368:1372	arg1	production					1319:1328	the production	1315:1328	the production of health-promoting short chain fatty acids	1315:1372	LLP-I could also promote the production of health-promoting short chain fatty acids.					
33232697	6	28	theme	potential	1526:1534	arg1	prebiotic					1536:1544	a potential prebiotic	1524:1544	a potential prebiotic in the functional food industry	1524:1576	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	6	28	theme	potential	1526:1534	arg1	LLP					1500:1502	LLP	1500:1502	LLP	1500:1502	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	0	29	theme	In	0:1	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	4	30	theme	microbiota	1278:1287	arg1	composition					1245:1255	composition	1245:1255	composition	1245:1255	Indeed, some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio significantly increased, suggesting that LLP-I could change the composition and abundance of gut microbiota.					
33232697	4	30	theme	microbiota	1278:1287	arg1	abundance					1261:1269	abundance	1261:1269	abundance	1261:1269	Indeed, some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio significantly increased, suggesting that LLP-I could change the composition and abundance of gut microbiota.					
33232697	2	31	theme	digestion	685:693	arg1	conditions					695:704	the gastrointestinal digestion conditions	664:704	the gastrointestinal digestion conditions	664:704	Results showed that the contents of reducing sugar of LLP slightly increased during the gastrointestinal digestion, and its molecular weight also slightly decreased, suggesting that LLP could be slightly degraded under the gastrointestinal digestion conditions.					
33232697	6	32	theme	in	1436:1437	arg1	digestion					1445:1453	the in vitro digestion and fecal fermentation behaviors	1432:1486	digestion	1445:1453	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	1	33	theme	polysaccharides	272:286	arg1	properties					258:267	physicochemical properties	242:267	physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation	242:394	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	2	34	theme	gastrointestinal	668:683	arg1	conditions					695:704	the gastrointestinal digestion conditions	664:704	the gastrointestinal digestion conditions	664:704	Results showed that the contents of reducing sugar of LLP slightly increased during the gastrointestinal digestion, and its molecular weight also slightly decreased, suggesting that LLP could be slightly degraded under the gastrointestinal digestion conditions.					
33232697	0	35	theme	simulated	9:17	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	3	36	theme	molecular	752:760	arg1	weight					762:767	the molecular weight	748:767	the molecular weight of the indigestible LLP (LLP-I)	748:799	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	0	37	theme	physicochemical	110:124	arg1	properties					126:135	physicochemical properties	110:135	physicochemical properties	110:135	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	5	38	theme	health-promoting	1333:1348	arg1	acids					1368:1372	health-promoting short chain fatty acids	1333:1372	health-promoting short chain fatty acids	1333:1372	LLP-I could also promote the production of health-promoting short chain fatty acids.					
33232697	1	39	theme	study	190:194	arg1	aim					178:180	The aim	174:180	The aim of this study	174:194	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	0	40	theme	fecal	33:37	arg1	fermentation					39:50	fecal fermentation	33:50	fecal fermentation	33:50	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	1	41	dep	in	320:321	arg1	vitro					323:327	vitro	323:327	vitro	323:327	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	1	42	from	changes	231:237	arg1	microbiota					433:442	human gut microbiota	423:442	human gut microbiota	423:442	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	0	43	from	loquat	76:81	arg1	polysaccharides					55:69	polysaccharides	55:69	polysaccharides from loquat	55:81	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	0	43	from	loquat	76:81	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	0	43	from	loquat	76:81	arg1	fermentation					39:50	fecal fermentation	33:50	fecal fermentation	33:50	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	4	44	theme	beneficial	1071:1080	arg1	Phascolarctobacterium					1140:1160	Phascolarctobacterium	1140:1160	Phascolarctobacterium	1140:1160	Indeed, some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio significantly increased, suggesting that LLP-I could change the composition and abundance of gut microbiota.					
33232697	4	44	theme	beneficial	1071:1080	arg1	Desulfovibrio					1167:1179	Desulfovibrio	1167:1179	Desulfovibrio	1167:1179	Indeed, some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio significantly increased, suggesting that LLP-I could change the composition and abundance of gut microbiota.					
33232697	4	44	theme	beneficial	1071:1080	arg1	Bifidobacterium					1123:1137	Bifidobacterium	1123:1137	Bifidobacterium	1123:1137	Indeed, some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio significantly increased, suggesting that LLP-I could change the composition and abundance of gut microbiota.					
33232697	4	44	theme	beneficial	1071:1080	arg1	Megamonas					1112:1120	Megamonas	1112:1120	Megamonas	1112:1120	Indeed, some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio significantly increased, suggesting that LLP-I could change the composition and abundance of gut microbiota.					
33232697	4	44	theme	beneficial	1071:1080	arg1	bacteria					1082:1089	some beneficial bacteria	1066:1089	some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio	1066:1179	Indeed, some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio significantly increased, suggesting that LLP-I could change the composition and abundance of gut microbiota.					
33232697	6	45	theme	fermentation	1465:1476	arg1	behaviors					1478:1486	the in vitro digestion and fecal fermentation behaviors	1432:1486	behaviors	1478:1486	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	4	46	dep	composition	1245:1255	arg1	the					1241:1243	the	1241:1243	the	1241:1243	Indeed, some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio significantly increased, suggesting that LLP-I could change the composition and abundance of gut microbiota.					
33232697	2	47	theme	gastrointestinal	533:548	arg1	digestion					550:558	the gastrointestinal digestion	529:558	the gastrointestinal digestion	529:558	Results showed that the contents of reducing sugar of LLP slightly increased during the gastrointestinal digestion, and its molecular weight also slightly decreased, suggesting that LLP could be slightly degraded under the gastrointestinal digestion conditions.					
33232697	0	48	theme	human	152:156	arg1	microbiota					162:171	human gut microbiota	152:171	human gut microbiota	152:171	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	1	49	theme	related	404:410	arg1	impacts					412:418	its related impacts	400:418	its related impacts on human gut microbiota	400:442	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	3	50	theme	fecal	728:732	arg1	fermentation					734:745	the fecal fermentation	724:745	the fecal fermentation	724:745	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	51	theme	indigestible	776:787	arg1	LLP					789:791	the indigestible LLP	772:791	the indigestible LLP (LLP-I)	772:799	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	51	theme	indigestible	776:787	arg1	LLP-I					794:798	LLP-I	794:798	LLP-I	794:798	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	4	52	theme	gut	1274:1276	arg1	microbiota					1278:1287	gut microbiota	1274:1287	gut microbiota	1274:1287	Indeed, some beneficial bacteria such as Megasphaera, Megamonas, Bifidobacterium, Phascolarctobacterium, and Desulfovibrio significantly increased, suggesting that LLP-I could change the composition and abundance of gut microbiota.					
33232697	3	53	theme	monosaccharides	861:875	arg1	ratio					840:844	the molar ratio	830:844	the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose,	830:955	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	54	theme	LLP	789:791	arg1	weight					762:767	the molecular weight	748:767	the molecular weight of the indigestible LLP (LLP-I)	748:799	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	2	55	theme	molecular	569:577	arg1	weight					579:584	its molecular weight	565:584	its molecular weight	565:584	Results showed that the contents of reducing sugar of LLP slightly increased during the gastrointestinal digestion, and its molecular weight also slightly decreased, suggesting that LLP could be slightly degraded under the gastrointestinal digestion conditions.					
33232697	0	56	from	changes	99:105	arg1	impacts					141:147	impacts	141:147	impacts on human gut microbiota	141:171	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	0	56	from	changes	99:105	arg1	properties					126:135	physicochemical properties	110:135	physicochemical properties	110:135	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	0	56	from	changes	99:105	arg1	microbiota					162:171	human gut microbiota	152:171	human gut microbiota	152:171	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	0	57	from	impacts	141:147	arg1	microbiota					162:171	human gut microbiota	152:171	human gut microbiota	152:171	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	0	58	theme	gut	158:160	arg1	microbiota					162:171	human gut microbiota	152:171	human gut microbiota	152:171	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	1	59	theme	human	423:427	arg1	microbiota					433:442	human gut microbiota	423:442	human gut microbiota	423:442	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	3	60	theme	molar	834:838	arg1	ratio					840:844	the molar ratio	830:844	the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose,	830:955	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	5	61	theme	chain	1356:1360	arg1	acids					1368:1372	health-promoting short chain fatty acids	1333:1372	health-promoting short chain fatty acids	1333:1372	LLP-I could also promote the production of health-promoting short chain fatty acids.					
33232697	3	62	theme	glucuronic	895:904	arg1	acid					906:909	glucuronic acid	895:909	glucuronic acid	895:909	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	2	63	theme	LLP	499:501	arg1	sugar					490:494	reducing sugar	481:494	reducing sugar of LLP	481:501	Results showed that the contents of reducing sugar of LLP slightly increased during the gastrointestinal digestion, and its molecular weight also slightly decreased, suggesting that LLP could be slightly degraded under the gastrointestinal digestion conditions.					
33232697	1	64	theme	gut	429:431	arg1	microbiota					433:442	human gut microbiota	423:442	human gut microbiota	423:442	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33232697	3	65	theme	constituent	849:859	arg1	monosaccharides					861:875	constituent monosaccharides	849:875	constituent monosaccharides of LLP-I	849:884	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	65	theme	constituent	849:859	arg1	galactose					931:939	galactose	931:939	galactose	931:939	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	65	theme	constituent	849:859	arg1	acid					906:909	glucuronic acid	895:909	glucuronic acid	895:909	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	65	theme	constituent	849:859	arg1	acid					925:928	galacturonic acid	912:928	galacturonic acid	912:928	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	3	65	theme	constituent	849:859	arg1	arabinose					946:954	arabinose	946:954	arabinose	946:954	Moreover, during the fecal fermentation, the molecular weight of the indigestible LLP (LLP-I) significantly decreased, and the molar ratio of constituent monosaccharides of LLP-I, such as glucuronic acid, galacturonic acid, galactose, and arabinose, significantly changed, indicating that LLP-I could be degraded and consumed by human gut microbiota.					
33232697	0	66	from	properties	126:135	arg1	microbiota					162:171	human gut microbiota	152:171	human gut microbiota	152:171	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	0	67	theme	polysaccharides	55:69	arg1	digestion					19:27	In vitro simulated digestion	0:27	In vitro simulated digestion	0:27	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	0	67	theme	polysaccharides	55:69	arg1	fermentation					39:50	fecal fermentation	33:50	fecal fermentation	33:50	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	0	68	from	digestion	19:27	arg1	loquat					76:81	loquat	76:81	loquat	76:81	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	0	69	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro simulated digestion and fecal fermentation of polysaccharides from loquat leaves: Dynamic changes in physicochemical properties and impacts on human gut microbiota.					
33232697	6	70	theme	food	1564:1567	arg1	industry					1569:1576	the functional food industry	1549:1576	the functional food industry	1549:1576	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	6	71	theme	LLP	1491:1493	arg1	digestion					1445:1453	the in vitro digestion and fecal fermentation behaviors	1432:1486	digestion	1445:1453	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	6	71	theme	LLP	1491:1493	arg1	behaviors					1478:1486	the in vitro digestion and fecal fermentation behaviors	1432:1486	behaviors	1478:1486	Results from this study are benefical to well understand the in vitro digestion and fecal fermentation behaviors of LLP, and LLP can be developed as a potential prebiotic in the functional food industry.					
33232697	1	72	theme	dynamic	223:229	arg1	changes					231:237	the dynamic changes	219:237	the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation	219:394	The aim of this study was to well understand the dynamic changes of physicochemical properties of polysaccharides from loquat leaves (LLP) during in vitro simulated saliva-gastrointestinal digestion and fecal fermentation and its related impacts on human gut microbiota.					
33371380	4	0	theme	surface	950:956	arg1	SSA					964:966	SSA	964:966	SSA	964:966	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	0	theme	surface	950:956	arg1	area					958:961	their specific surface area	935:961	their specific surface area (SSA)	935:967	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	3	1	theme	aqueous	546:552	arg1	solution					554:561	aqueous solution	546:561	aqueous solution of sodium alginate	546:580	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	0	2	theme	Response	72:79	arg1	Properties					9:18	Physical Properties	0:18	Physical Properties	0:18	Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials.					
33371380	0	2	theme	Response	72:79	arg1	Analysis					30:37	Chemical Analysis	21:37	Chemical Analysis	21:37	Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials.					
33371380	0	2	theme	Response	72:79	arg1	Evaluation					44:53	Evaluation	44:53	Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials	44:134	Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials.					
33371380	6	3	theme	promising	1532:1540	arg1	composites					1516:1525	the obtained composites	1503:1525	the obtained composites	1503:1525	All the results obtained in this work showed that the obtained composites were promising materials to be used as an antimicrobial wound dressing.					
33371380	6	3	theme	promising	1532:1540	arg1	materials					1542:1550	promising materials	1532:1550	promising materials	1532:1550	All the results obtained in this work showed that the obtained composites were promising materials to be used as an antimicrobial wound dressing.					
33371380	1	4	theme	antibacterial	267:279	arg1	polymers					281:288	biodegradable and antibacterial polymers	249:288	biodegradable and antibacterial polymers that can be used in selected biomedical applications	249:341	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
33371380	0	5	theme	Polylactide/Alginate/Copper	88:114	arg1	Materials					126:134	New Polylactide/Alginate/Copper Composite Materials	84:134	New Polylactide/Alginate/Copper Composite Materials	84:134	Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials.					
33371380	5	6	theme	fungal	1432:1437	arg1	species					1444:1450	fungal mold species	1432:1450	fungal mold species	1432:1450	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	4	7	theme	Fourier-transform	861:877	arg1	spectroscopy					888:899	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	5	8	theme	Gram-negative	1283:1295	arg1	colonies					1271:1278	colonies	1271:1278	colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria	1271:1344	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	4	9	theme	volumes	989:995	arg1	determination					918:930	determination	918:930	determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples)	918:1173	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	0	10	theme	New	84:86	arg1	Materials					126:134	New Polylactide/Alginate/Copper Composite Materials	84:134	New Polylactide/Alginate/Copper Composite Materials	84:134	Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials.					
33371380	5	11	theme	antifungal	1350:1359	arg1	tests					1376:1380	antifungal susceptibility tests	1350:1380	antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species	1350:1450	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	0	12	theme	Materials	126:134	arg1	Response					72:79	Antimicrobial Response	58:79	Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials	58:134	Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials.					
33371380	1	13	used	used	302:305	arg2	polymers					281:288	biodegradable and antibacterial polymers	249:288	biodegradable and antibacterial polymers that can be used in selected biomedical applications	249:341	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
33371380	5	14	theme	mold	1439:1442	arg1	species					1444:1450	fungal mold species	1432:1450	fungal mold species	1432:1450	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	3	15	theme	alginate	573:580	arg1	solution					554:561	aqueous solution	546:561	aqueous solution of sodium alginate	546:580	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	1	16	theme	antibiotic-resistant	177:196	arg1	microorganisms					198:211	antibiotic-resistant microorganisms	177:211	antibiotic-resistant microorganisms	177:211	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
33371380	2	17	theme	PLA-ALG-Cu2+	431:442	arg1	fibers					455:460	antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	388:460	antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	388:460	The present work describes the synthesis of antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers and their characterization.					
33371380	0	18	theme	Composite	116:124	arg1	Materials					126:134	New Polylactide/Alginate/Copper Composite Materials	84:134	New Polylactide/Alginate/Copper Composite Materials	84:134	Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials.					
33371380	4	19	theme	infrared	879:886	arg1	spectroscopy					888:899	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	5	20	theme	activity	1237:1244	arg1	tests					1257:1261	in vitro antimicrobial activity evaluation tests	1214:1261	in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria	1214:1344	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	5	21	theme	bacteria	1337:1344	arg1	colonies					1271:1278	colonies	1271:1278	colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria	1271:1344	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	3	22	with	fibers	657:662	arg1	ions					676:679	Cu(II) ions	669:679	Cu(II) ions	669:679	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	4	23	theme	pore	984:987	arg1	volumes					989:995	total/average pore volumes	970:995	total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET))	970:1068	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	5	24	dep	niger	1402:1406	arg1	species					1444:1450	fungal mold species	1432:1450	fungal mold species	1432:1450	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	2	25	theme	alginate	421:428	arg1	fibers					455:460	antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	388:460	antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	388:460	The present work describes the synthesis of antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers and their characterization.					
33371380	0	26	theme	Physical	0:7	arg1	Properties					9:18	Physical Properties	0:18	Physical Properties	0:18	Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials.					
33371380	4	27	theme	ATR-FTIR	901:908	arg1	spectroscopy					888:899	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	28	theme	ultraviolet	1127:1137	arg1	UPF					1158:1160	UPF	1158:1160	UPF	1158:1160	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	28	theme	ultraviolet	1127:1137	arg1	factor					1150:1155	the ultraviolet protection factor	1123:1155	the ultraviolet protection factor (UPF) of samples	1123:1172	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	3	29	theme	alginate	618:625	arg1	chains					627:632	alginate chains	618:632	alginate chains within the polylactide fibers with Cu(II) ions	618:679	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	2	30	theme	polylactide-copper	402:419	arg1	fibers					455:460	antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	388:460	antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	388:460	The present work describes the synthesis of antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers and their characterization.					
33371380	0	31	theme	Chemical	21:28	arg1	Analysis					30:37	Chemical Analysis	21:37	Chemical Analysis	21:37	Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials.					
33371380	4	32	dep	radiation	1095:1103	arg1	determination					1106:1118	determination	1106:1118	determination of the ultraviolet protection factor (UPF) of samples	1106:1172	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	3	33	theme	ionic	595:599	arg1	cross-linking					601:613	ionic cross-linking	595:613	ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions	595:679	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	5	34	theme	Gram-positive	1311:1323	arg1	bacteria					1337:1344	Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria	1283:1344	bacteria	1337:1344	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	6	35	theme	wound	1583:1587	arg1	dressing					1589:1596	an antimicrobial wound dressing	1566:1596	an antimicrobial wound dressing	1566:1596	All the results obtained in this work showed that the obtained composites were promising materials to be used as an antimicrobial wound dressing.					
33371380	4	36	theme	scanning	772:779	arg1	SEM					802:804	SEM	802:804	SEM	802:804	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	36	theme	scanning	772:779	arg1	microscopy					790:799	scanning electron microscopy	772:799	scanning electron microscopy (SEM)	772:805	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	37	theme	protection	1139:1148	arg1	UPF					1158:1160	UPF	1158:1160	UPF	1158:1160	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	37	theme	protection	1139:1148	arg1	factor					1150:1155	the ultraviolet protection factor	1123:1155	the ultraviolet protection factor (UPF) of samples	1123:1172	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	2	38	theme	fibers	455:460	arg1	synthesis					375:383	the synthesis	371:383	the synthesis of antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	371:460	The present work describes the synthesis of antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers and their characterization.					
33371380	2	38	theme	fibers	455:460	arg1	characterization					472:487	their characterization	466:487	their characterization	466:487	The present work describes the synthesis of antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers and their characterization.					
33371380	4	39	theme	Brunauer-Emmett-Teller	1033:1054	arg1	method					1056:1061	the 5-point Brunauer-Emmett-Teller method	1021:1061	the 5-point Brunauer-Emmett-Teller method (BET)	1021:1067	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	39	theme	Brunauer-Emmett-Teller	1033:1054	arg1	BET					1064:1066	BET	1064:1066	BET	1064:1066	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	40	theme	factor	1150:1155	arg1	determination					1106:1118	determination	1106:1118	determination of the ultraviolet protection factor (UPF) of samples	1106:1172	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	41	theme	total/average	970:982	arg1	volumes					989:995	total/average pore volumes	970:995	total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET))	970:1068	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	5	42	theme	antimicrobial	1223:1235	arg1	tests					1257:1261	in vitro antimicrobial activity evaluation tests	1214:1261	in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria	1214:1344	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	4	43	theme	total	844:848	arg1	spectroscopy					888:899	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	1	44	theme	microorganisms	198:211	arg1	expansion					164:172	an expansion	161:172	an expansion of antibiotic-resistant microorganisms	161:211	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
33371380	2	45	theme	composite	445:453	arg1	fibers					455:460	antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	388:460	antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	388:460	The present work describes the synthesis of antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers and their characterization.					
33371380	4	46	theme	5-point	1025:1031	arg1	method					1056:1061	the 5-point Brunauer-Emmett-Teller method	1021:1061	the 5-point Brunauer-Emmett-Teller method (BET)	1021:1067	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	46	theme	5-point	1025:1031	arg1	BET					1064:1066	BET	1064:1066	BET	1064:1066	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	1	47	theme	selected	310:317	arg1	applications					330:341	selected biomedical applications	310:341	selected biomedical applications	310:341	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
33371380	5	48	dep	Gram-negative	1283:1295	arg1	coli					1301:1304	E. coli	1298:1304	E. coli	1298:1304	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	3	49	theme	polylactide	645:655	arg1	fibers					657:662	the polylactide fibers	641:662	the polylactide fibers with Cu(II) ions	641:679	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	5	50	dep	bacteria	1337:1344	arg1	aureus					1329:1334	S. aureus	1326:1334	S. aureus	1326:1334	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	4	51	theme	electron	781:788	arg1	SEM					802:804	SEM	802:804	SEM	802:804	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	51	theme	electron	781:788	arg1	microscopy					790:799	scanning electron microscopy	772:799	scanning electron microscopy (SEM)	772:805	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	5	52	dep	in	1214:1215	arg1	vitro					1217:1221	vitro	1217:1221	vitro	1217:1221	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	1	53	theme	biomedical	319:328	arg1	applications					330:341	selected biomedical applications	310:341	selected biomedical applications	310:341	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
33371380	4	54	theme	specific	941:948	arg1	SSA					964:966	SSA	964:966	SSA	964:966	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	54	theme	specific	941:948	arg1	area					958:961	their specific surface area	935:961	their specific surface area (SSA)	935:967	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	3	55	theme	chains	627:632	arg1	cross-linking					601:613	ionic cross-linking	595:613	ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions	595:679	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	4	56	theme	method	1056:1061	arg1	application					1006:1016	application	1006:1016	application of the 5-point Brunauer-Emmett-Teller method (BET)	1006:1067	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	3	57	theme	composite	703:711	arg1	fibers					713:718	PLA-ALG-Cu2+ composite fibers	690:718	PLA-ALG-Cu2+ composite fibers	690:718	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	4	58	theme	reflection	850:859	arg1	spectroscopy					888:899	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	6	59	theme	obtained	1507:1514	arg1	composites					1516:1525	the obtained composites	1503:1525	the obtained composites	1503:1525	All the results obtained in this work showed that the obtained composites were promising materials to be used as an antimicrobial wound dressing.					
33371380	6	59	theme	obtained	1507:1514	arg1	materials					1542:1550	promising materials	1532:1550	promising materials	1532:1550	All the results obtained in this work showed that the obtained composites were promising materials to be used as an antimicrobial wound dressing.					
33371380	4	60	theme	area	958:961	arg1	determination					918:930	determination	918:930	determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples)	918:1173	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	61	theme	UV/VIS	808:813	arg1	transmittance					815:827	UV/VIS transmittance	808:827	UV/VIS transmittance	808:827	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	3	62	theme	Cu	669:670	arg1	ions					676:679	Cu(II) ions	669:679	Cu(II) ions	669:679	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	4	63	theme	attenuated	833:842	arg1	spectroscopy					888:899	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR	833:908	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	1	64	theme	recent	140:145	arg1	years					147:151	recent years	140:151	recent years	140:151	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
33371380	4	65	theme	UV	1092:1093	arg1	radiation					1095:1103	UV radiation	1092:1103	UV radiation (determination of the ultraviolet protection factor (UPF) of samples)	1092:1173	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	1	66	theme	growing	229:235	arg1	interest					237:244	growing interest	229:244	growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications	229:341	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
33371380	1	67	from	interest	237:244	arg1	years					147:151	recent years	140:151	recent years	140:151	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
33371380	1	67	from	interest	237:244	arg1	polymers					281:288	biodegradable and antibacterial polymers	249:288	biodegradable and antibacterial polymers that can be used in selected biomedical applications	249:341	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
33371380	4	68	theme	samples	1166:1172	arg1	UPF					1158:1160	UPF	1158:1160	UPF	1158:1160	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	4	68	theme	samples	1166:1172	arg1	factor					1150:1155	the ultraviolet protection factor	1123:1155	the ultraviolet protection factor (UPF) of samples	1123:1172	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	3	69	theme	PLA	532:534	arg1	fibers					536:541	PLA fibers	532:541	PLA fibers	532:541	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	2	70	theme	present	348:354	arg1	work					356:359	The present work	344:359	The present work	344:359	The present work describes the synthesis of antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers and their characterization.					
33371380	0	71	theme	Antimicrobial	58:70	arg1	Response					72:79	Antimicrobial Response	58:79	Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials	58:134	Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials.					
33371380	3	72	theme	PLA-ALG-Cu2+	690:701	arg1	fibers					713:718	PLA-ALG-Cu2+ composite fibers	690:718	PLA-ALG-Cu2+ composite fibers	690:718	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	5	73	theme	susceptibility	1361:1374	arg1	tests					1376:1380	antifungal susceptibility tests	1350:1380	antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species	1350:1450	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	2	74	theme	antimicrobial	388:400	arg1	fibers					455:460	antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	388:460	antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers	388:460	The present work describes the synthesis of antimicrobial polylactide-copper alginate (PLA-ALG-Cu2+) composite fibers and their characterization.					
33371380	5	75	theme	evaluation	1246:1255	arg1	tests					1257:1261	in vitro antimicrobial activity evaluation tests	1214:1261	in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria	1214:1344	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	5	76	theme	in	1214:1215	arg1	tests					1257:1261	in vitro antimicrobial activity evaluation tests	1214:1261	in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria	1214:1344	The composites were also subjected to in vitro antimicrobial activity evaluation tests against colonies of Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria and antifungal susceptibility tests against Aspergillus niger and Chaetomium globosum fungal mold species.					
33371380	6	77	theme	antimicrobial	1569:1581	arg1	dressing					1589:1596	an antimicrobial wound dressing	1566:1596	an antimicrobial wound dressing	1566:1596	All the results obtained in this work showed that the obtained composites were promising materials to be used as an antimicrobial wound dressing.					
33371380	3	78	theme	sodium	566:571	arg1	alginate					573:580	sodium alginate	566:580	sodium alginate	566:580	The composites were prepared by immersing PLA fibers in aqueous solution of sodium alginate, followed by ionic cross-linking of alginate chains within the polylactide fibers with Cu(II) ions to yield PLA-ALG-Cu2+ composite fibers.					
33371380	4	79	theme	ability	1075:1081	arg1	determination					918:930	determination	918:930	determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples)	918:1173	The composites, so prepared, were characterized by scanning electron microscopy (SEM), UV/VIS transmittance and attenuated total reflection Fourier-transform infrared spectroscopy ATR-FTIR, and by determination of their specific surface area (SSA), total/average pore volumes (through application of the 5-point Brunauer-Emmett-Teller method (BET)), and ability to block UV radiation (determination of the ultraviolet protection factor (UPF) of samples).					
33371380	1	80	theme	biodegradable	249:261	arg1	polymers					281:288	biodegradable and antibacterial polymers	249:288	biodegradable and antibacterial polymers that can be used in selected biomedical applications	249:341	In recent years, due to an expansion of antibiotic-resistant microorganisms, there has been growing interest in biodegradable and antibacterial polymers that can be used in selected biomedical applications.					
32241399	2	0	theme	composite	482:490	arg1	films					492:496	the composite films	478:496	the composite films	478:496	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films were compared.					
32241399	5	1	theme	CSNPs	772:776	arg1	inclusion					752:760	The inclusion	748:760	The inclusion of CS and CSNPs	748:776	The inclusion of CS and CSNPs both reduced the water solubility and water vapor permeability.					
32241399	7	2	theme	composite	1157:1165	arg1	membranes					1167:1175	CS composite membranes	1154:1175	CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm	1154:1233	In addition, CSNPs-embedded membranes exhibited prominent antibacterial properties against Escherichia coli and Staphylococcus aureus, which were much greater than those of CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm.					
32241399	7	3	theme	CSNPs-embedded	994:1007	arg1	membranes					1009:1017	CSNPs-embedded membranes	994:1017	CSNPs-embedded membranes	994:1017	In addition, CSNPs-embedded membranes exhibited prominent antibacterial properties against Escherichia coli and Staphylococcus aureus, which were much greater than those of CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm.					
32241399	1	4	theme	bulk	172:175	arg1	CS					187:188	CS	187:188	CS	187:188	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	4	theme	bulk	172:175	arg1	chitosan					177:184	bulk chitosan	172:184	bulk chitosan (CS)	172:189	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	5	theme	chitosan	177:184	arg1	nanoparticles					204:216	bulk chitosan (CS) and chitosan nanoparticles	172:216	bulk chitosan (CS) and chitosan nanoparticles (CSNPs)	172:224	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	5	theme	chitosan	177:184	arg1	CSNPs					219:223	CSNPs	219:223	CSNPs	219:223	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	6	theme	composites	341:350	arg1	films					352:356	the composites films	337:356	the composites films	337:356	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	3	7	theme	mechanical	543:552	arg1	properties					554:563	the mechanical properties	539:563	the mechanical properties	539:563	CS incorporation improved the mechanical properties, as the maximum tensile strength was increased to 130.55 ± 9.42 MPa.					
32241399	4	8	theme	water	673:677	arg1	diffusion					679:687	water diffusion	673:687	water diffusion	673:687	The dense structure of CSNPs prevented water diffusion and lessened the water content of the composite membranes.					
32241399	6	9	theme	ultraviolet-barrier	909:927	arg1	properties					929:938	better ultraviolet-barrier properties	902:938	better ultraviolet-barrier properties	902:938	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	7	10	theme	CS	1154:1155	arg1	membranes					1167:1175	CS composite membranes	1154:1175	CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm	1154:1233	In addition, CSNPs-embedded membranes exhibited prominent antibacterial properties against Escherichia coli and Staphylococcus aureus, which were much greater than those of CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm.					
32241399	1	11	theme	bacterial	276:284	arg1	cellulose/poly					286:299	bacterial cellulose/poly	276:299	bacterial cellulose/poly(vinyl alcohol)	276:314	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	11	theme	bacterial	276:284	arg1	alcohol					307:313	vinyl alcohol	301:313	vinyl alcohol	301:313	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	11	theme	bacterial	276:284	arg1	film					328:331	the base film	319:331	the base film	319:331	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	0	12	theme	comparative	118:128	arg1	study					130:134	A comparative study	116:134	Characterization of bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study.	0:135	Characterization of bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study.					
32241399	6	13	theme	better	902:907	arg1	properties					929:938	better ultraviolet-barrier properties	902:938	better ultraviolet-barrier properties	902:938	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	6	14	contain	had	898:900	arg1	CSNPs					892:896	CSNPs	892:896	CSNPs	892:896	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	6	14	contain	had	898:900	arg2	properties					929:938	better ultraviolet-barrier properties	902:938	better ultraviolet-barrier properties	902:938	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	2	15	theme	mechanical	397:406	arg1	properties					464:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties	373:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films	373:496	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films were compared.					
32241399	1	16	contain	containing	161:170	arg2	CSNPs					219:223	CSNPs	219:223	CSNPs	219:223	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	16	contain	containing	161:170	arg2	nanoparticles					204:216	bulk chitosan (CS) and chitosan nanoparticles	172:216	bulk chitosan (CS) and chitosan nanoparticles (CSNPs)	172:224	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	16	contain	containing	161:170	arg1	films					155:159	Ternary composite films	137:159	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations	137:254	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	7	17	theme	maximum	1184:1190	arg1	diameter					1207:1214	a maximum bacteriostatic diameter	1182:1214	a maximum bacteriostatic diameter of 10.33 ± 1.55 mm	1182:1233	In addition, CSNPs-embedded membranes exhibited prominent antibacterial properties against Escherichia coli and Staphylococcus aureus, which were much greater than those of CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm.					
32241399	7	18	with	membranes	1167:1175	arg1	diameter					1207:1214	a maximum bacteriostatic diameter	1182:1214	a maximum bacteriostatic diameter of 10.33 ± 1.55 mm	1182:1233	In addition, CSNPs-embedded membranes exhibited prominent antibacterial properties against Escherichia coli and Staphylococcus aureus, which were much greater than those of CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm.					
32241399	7	19	theme	10.33 ± 1.55 mm	1219:1233	arg1	diameter					1207:1214	a maximum bacteriostatic diameter	1182:1214	a maximum bacteriostatic diameter of 10.33 ± 1.55 mm	1182:1233	In addition, CSNPs-embedded membranes exhibited prominent antibacterial properties against Escherichia coli and Staphylococcus aureus, which were much greater than those of CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm.					
32241399	2	20	theme	micromorphology	377:391	arg1	properties					464:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties	373:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films	373:496	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films were compared.					
32241399	5	21	theme	water	816:820	arg1	permeability					828:839	the water solubility and water vapor permeability	791:839	permeability	828:839	The inclusion of CS and CSNPs both reduced the water solubility and water vapor permeability.					
32241399	4	22	theme	composite	727:735	arg1	membranes					737:745	the composite membranes	723:745	the composite membranes	723:745	The dense structure of CSNPs prevented water diffusion and lessened the water content of the composite membranes.					
32241399	3	23	theme	maximum	573:579	arg1	strength					589:596	the maximum tensile strength	569:596	the maximum tensile strength	569:596	CS incorporation improved the mechanical properties, as the maximum tensile strength was increased to 130.55 ± 9.42 MPa.					
32241399	0	24	theme	cellulose	30:38	arg1	films					50:54	bacterial cellulose composite films	20:54	bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles	20:113	Characterization of bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study.					
32241399	0	25	dep	chitosan	79:86	arg1	nanoparticles					101:113	nanoparticles	101:113	nanoparticles	101:113	Characterization of bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study.					
32241399	1	26	theme	chitosan	195:202	arg1	nanoparticles					204:216	bulk chitosan (CS) and chitosan nanoparticles	172:216	bulk chitosan (CS) and chitosan nanoparticles (CSNPs)	172:224	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	26	theme	chitosan	195:202	arg1	CSNPs					219:223	CSNPs	219:223	CSNPs	219:223	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	0	27	theme	bacterial	20:28	arg1	films					50:54	bacterial cellulose composite films	20:54	bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles	20:113	Characterization of bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study.					
32241399	1	28	theme	vinyl	301:305	arg1	cellulose/poly					286:299	bacterial cellulose/poly	276:299	bacterial cellulose/poly(vinyl alcohol)	276:314	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	28	theme	vinyl	301:305	arg1	alcohol					307:313	vinyl alcohol	301:313	vinyl alcohol	301:313	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	4	29	theme	water	706:710	arg1	content					712:718	the water content	702:718	the water content of the composite membranes	702:745	The dense structure of CSNPs prevented water diffusion and lessened the water content of the composite membranes.					
32241399	4	30	theme	dense	638:642	arg1	structure					644:652	The dense structure	634:652	The dense structure of CSNPs	634:661	The dense structure of CSNPs prevented water diffusion and lessened the water content of the composite membranes.					
32241399	0	31	theme	films	50:54	arg1	Characterization					0:15	Characterization	0:15	Characterization of bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study.	0:135	Characterization of bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study.					
32241399	4	32	theme	CSNPs	657:661	arg1	structure					644:652	The dense structure	634:652	The dense structure of CSNPs	634:661	The dense structure of CSNPs prevented water diffusion and lessened the water content of the composite membranes.					
32241399	2	33	theme	physical	409:416	arg1	properties					464:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties	373:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films	373:496	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films were compared.					
32241399	0	34	theme	composite	40:48	arg1	films					50:54	bacterial cellulose composite films	20:54	bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles	20:113	Characterization of bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study.					
32241399	7	35	theme	bacteriostatic	1192:1205	arg1	diameter					1207:1214	a maximum bacteriostatic diameter	1182:1214	a maximum bacteriostatic diameter of 10.33 ± 1.55 mm	1182:1233	In addition, CSNPs-embedded membranes exhibited prominent antibacterial properties against Escherichia coli and Staphylococcus aureus, which were much greater than those of CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm.					
32241399	2	36	theme	antibacterial	429:441	arg1	properties					464:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties	373:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films	373:496	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films were compared.					
32241399	5	37	theme	vapor	822:826	arg1	permeability					828:839	the water solubility and water vapor permeability	791:839	permeability	828:839	The inclusion of CS and CSNPs both reduced the water solubility and water vapor permeability.					
32241399	2	38	theme	barrier	456:462	arg1	properties					464:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties	373:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films	373:496	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films were compared.					
32241399	6	39	theme	good	867:870	arg1	transparency					872:883	good transparency	867:883	good transparency	867:883	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	3	40	theme	tensile	581:587	arg1	strength					589:596	the maximum tensile strength	569:596	the maximum tensile strength	569:596	CS incorporation improved the mechanical properties, as the maximum tensile strength was increased to 130.55 ± 9.42 MPa.					
32241399	2	41	theme	optical	448:454	arg1	properties					464:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties	373:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films	373:496	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films were compared.					
32241399	6	42	theme	transmittance	951:963	arg1	transmittance					951:963	transmittance	951:963	transmittance	951:963	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	6	42	theme	transmittance	951:963	arg1	%					946:946	3.84 %	941:946	3.84 % of transmittance at 200-280 nm	941:977	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	7	43	theme	prominent	1029:1037	arg1	properties					1053:1062	prominent antibacterial properties	1029:1062	prominent antibacterial properties	1029:1062	In addition, CSNPs-embedded membranes exhibited prominent antibacterial properties against Escherichia coli and Staphylococcus aureus, which were much greater than those of CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm.					
32241399	1	44	theme	base	323:326	arg1	film					328:331	the base film	319:331	the base film	319:331	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	1	44	theme	base	323:326	arg1	cellulose/poly					286:299	bacterial cellulose/poly	276:299	bacterial cellulose/poly(vinyl alcohol)	276:314	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	6	45	from	200-280 nm	968:977	arg1	transmittance					951:963	transmittance	951:963	transmittance	951:963	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	6	45	from	200-280 nm	968:977	arg1	%					946:946	3.84 %	941:946	3.84 % of transmittance at 200-280 nm	941:977	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	5	46	theme	water	795:799	arg1	solubility					801:810	the water solubility and water vapor permeability	791:839	solubility	801:810	The inclusion of CS and CSNPs both reduced the water solubility and water vapor permeability.					
32241399	7	47	theme	antibacterial	1039:1051	arg1	properties					1053:1062	prominent antibacterial properties	1029:1062	prominent antibacterial properties	1029:1062	In addition, CSNPs-embedded membranes exhibited prominent antibacterial properties against Escherichia coli and Staphylococcus aureus, which were much greater than those of CS composite membranes with a maximum bacteriostatic diameter of 10.33 ± 1.55 mm.					
32241399	1	48	theme	Ternary	137:143	arg1	films					155:159	Ternary composite films	137:159	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations	137:254	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	6	49	dep	had	898:900	arg1	transmittance					951:963	transmittance	951:963	transmittance	951:963	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	6	49	dep	had	898:900	arg1	%					946:946	3.84 %	941:946	3.84 % of transmittance at 200-280 nm	941:977	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	6	50	theme	CS-doped	842:849	arg1	films					851:855	CS-doped films	842:855	CS-doped films	842:855	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	1	51	theme	composite	145:153	arg1	films					155:159	Ternary composite films	137:159	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations	137:254	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	4	52	theme	membranes	737:745	arg1	content					712:718	the water content	702:718	the water content of the composite membranes	702:745	The dense structure of CSNPs prevented water diffusion and lessened the water content of the composite membranes.					
32241399	5	53	theme	CS	765:766	arg1	inclusion					752:760	The inclusion	748:760	The inclusion of CS and CSNPs	748:776	The inclusion of CS and CSNPs both reduced the water solubility and water vapor permeability.					
32241399	2	54	theme	chemical	419:426	arg1	properties					464:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties	373:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films	373:496	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films were compared.					
32241399	3	55	theme	CS	513:514	arg1	incorporation					516:528	CS incorporation	513:528	CS incorporation	513:528	CS incorporation improved the mechanical properties, as the maximum tensile strength was increased to 130.55 ± 9.42 MPa.					
32241399	1	56	theme	different	231:239	arg1	concentrations					241:254	different concentrations	231:254	different concentrations	231:254	Ternary composite films containing bulk chitosan (CS) and chitosan nanoparticles (CSNPs) with different concentrations were prepared using bacterial cellulose/poly(vinyl alcohol) as the base film and the composites films were compared.					
32241399	2	57	theme	films	492:496	arg1	properties					464:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties	373:473	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films	373:496	The micromorphology and mechanical, physical, chemical, antibacterial, and optical barrier properties of the composite films were compared.					
32241399	0	58	dep	Characterization	0:15	arg1	study					130:134	A comparative study	116:134	Characterization of bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study.	0:135	Characterization of bacterial cellulose composite films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study.					
32241399	6	59	contain	possessed	857:865	arg1	films					851:855	CS-doped films	842:855	CS-doped films	842:855	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
32241399	6	59	contain	possessed	857:865	arg2	transparency					872:883	good transparency	867:883	good transparency	867:883	CS-doped films possessed good transparency, while CSNPs had better ultraviolet-barrier properties (3.84 % of transmittance at 200-280 nm).					
34315131	5	0	theme	3-L	920:922	arg1	Erlenmeyers					924:934	3-L Erlenmeyers	920:934	3-L Erlenmeyers	920:934	Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers.					
34315131	6	1	theme	green	1037:1041	arg1	chemistry					1043:1051	green chemistry	1037:1051	green chemistry	1037:1051	Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts.					
34315131	0	2	theme	hydrothermal	118:129	arg1	pretreatment					131:142	hydrothermal pretreatment	118:142	hydrothermal pretreatment of sugarcane bagasse	118:163	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse.					
34315131	7	3	theme	sugarcane	1141:1149	arg1	valorisation					1159:1170	a future proper sugarcane bagasse valorisation	1125:1170	a future proper sugarcane bagasse valorisation for pediocin production	1125:1194	This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.					
34315131	4	4	from	increase	613:620	arg1	expression					629:638	the expression	625:638	the expression of PA-1 genes and a non-optimized purification step sequence	625:699	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	7	5	theme	bagasse	1151:1157	arg1	valorisation					1159:1170	a future proper sugarcane bagasse valorisation	1125:1170	a future proper sugarcane bagasse valorisation for pediocin production	1125:1194	This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.					
34315131	3	6	theme	100	517:519	arg1	%					520:520	%	520:520	%	520:520	Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1.					
34315131	7	7	theme	future	1127:1132	arg1	valorisation					1159:1170	a future proper sugarcane bagasse valorisation	1125:1170	a future proper sugarcane bagasse valorisation for pediocin production	1125:1194	This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.					
34315131	4	8	theme	genes	648:652	arg1	expression					629:638	the expression	625:638	the expression of PA-1 genes and a non-optimized purification step sequence	625:699	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	4	9	theme	step	687:690	arg1	sequence					692:699	a non-optimized purification step sequence	658:699	a non-optimized purification step sequence	658:699	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	7	10	theme	pediocin	1176:1183	arg1	production					1185:1194	pediocin production	1176:1194	pediocin production	1176:1194	This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.					
34315131	3	11	theme	Pediococcus	438:448	arg1	ET34					463:466	Pediococcus pentosaceous ET34	438:466	Pediococcus pentosaceous ET34	438:466	Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1.					
34315131	0	12	theme	Pediocin	0:7	arg1	PA-1					9:12	Pediocin PA-1	0:12	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate	0:102	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse.					
34315131	1	13	theme	foodborne	203:211	arg1	pathogens					213:221	the foodborne pathogens	199:221	the foodborne pathogens of most concern for food safety	199:253	Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety.					
34315131	0	14	theme	PA-1	9:12	arg1	production					14:23	Pediocin PA-1 production	0:23	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate	0:102	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse.					
34315131	1	15	theme	pathogens	213:221	arg1	pathogens					213:221	the foodborne pathogens	199:221	the foodborne pathogens of most concern for food safety	199:253	Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety.					
34315131	1	15	theme	pathogens	213:221	arg1	one					192:194	one	192:194	one	192:194	Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety.					
34315131	3	16	theme	sugarcane	417:425	arg1	bagasse					427:433	sugarcane bagasse	417:433	sugarcane bagasse	417:433	Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1.					
34315131	4	17	theme	hydrolysate-containing	573:594	arg1	medium					596:601	hydrolysate-containing medium	573:601	hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence	573:699	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	4	18	theme	pure	738:741	arg1	%					764:764	purity > 95%	753:764	purity > 95%	753:764	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	4	18	theme	pure	738:741	arg1	pediocin					743:750	pure pediocin	738:750	pure pediocin (purity > 95%)	738:765	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	2	19	theme	natural	323:329	arg1	bacteriocins					288:299	bacteriocins	288:299	bacteriocins	288:299	To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives.					
34315131	2	19	theme	natural	323:329	arg1	bio-preservatives					331:347	natural bio-preservatives	323:347	natural bio-preservatives	323:347	To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives.					
34315131	2	20	from	presence	269:276	arg1	foods					281:285	foods	281:285	foods	281:285	To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives.					
34315131	4	21	theme	sequence	692:699	arg1	expression					629:638	the expression	625:638	the expression of PA-1 genes and a non-optimized purification step sequence	625:699	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	3	22	theme	hemicellulose	388:400	arg1	hydrolysate					402:412	hemicellulose hydrolysate	388:412	hemicellulose hydrolysate of sugarcane bagasse	388:433	Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1.					
34315131	5	23	theme	composite	825:833	arg1	design					835:840	a central composite design	815:840	a central composite design using temperature and hydrolysate % as independent variables	815:901	Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers.					
34315131	0	24	theme	bagasse	157:163	arg1	pretreatment					131:142	hydrothermal pretreatment	118:142	hydrothermal pretreatment of sugarcane bagasse	118:163	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse.					
34315131	1	25	theme	most	226:229	arg1	concern					231:237	most concern	226:237	most concern for food safety	226:253	Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety.					
34315131	6	26	theme	sugar-ethanol	988:1000	arg1	industry					1002:1009	sugar-ethanol industry	988:1009	sugar-ethanol industry	988:1009	Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts.					
34315131	0	27	theme	sugarcane	147:155	arg1	bagasse					157:163	sugarcane bagasse	147:163	sugarcane bagasse	147:163	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse.					
34315131	1	28	theme	concern	231:237	arg1	pathogens					213:221	the foodborne pathogens	199:221	the foodborne pathogens of most concern for food safety	199:253	Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety.					
34315131	0	29	theme	pentosaceus	40:50	arg1	ET34					52:55	Pediococcus pentosaceus ET34	28:55	Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate	28:102	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse.					
34315131	5	30	theme	hydrolysate	864:874	arg1	temperature					848:858	temperature	848:858	temperature	848:858	Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers.					
34315131	5	30	theme	hydrolysate	864:874	arg1	%					876:876	hydrolysate %	864:876	hydrolysate %	864:876	Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers.					
34315131	5	30	theme	hydrolysate	864:874	arg1	variables					893:901	independent variables	881:901	independent variables	881:901	Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers.					
34315131	4	31	theme	non-optimized	660:672	arg1	step					687:690	a non-optimized purification step	658:690	a non-optimized purification step sequence	658:699	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	0	32	theme	Pediococcus	28:38	arg1	ET34					52:55	Pediococcus pentosaceus ET34	28:55	Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate	28:102	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse.					
34315131	5	33	theme	independent	881:891	arg1	temperature					848:858	temperature	848:858	temperature	848:858	Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers.					
34315131	5	33	theme	independent	881:891	arg1	%					876:876	hydrolysate %	864:876	hydrolysate %	864:876	Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers.					
34315131	5	33	theme	independent	881:891	arg1	variables					893:901	independent variables	881:901	independent variables	881:901	Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers.					
34315131	3	34	theme	pentosaceous	450:461	arg1	ET34					463:466	Pediococcus pentosaceous ET34	438:466	Pediococcus pentosaceous ET34	438:466	Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1.					
34315131	1	35	theme	food	243:246	arg1	safety					248:253	food safety	243:253	food safety	243:253	Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety.					
34315131	3	36	theme	bagasse	427:433	arg1	hydrolysate					402:412	hemicellulose hydrolysate	388:412	hemicellulose hydrolysate of sugarcane bagasse	388:433	Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1.					
34315131	6	37	theme	scaled-up	960:968	arg1	implementation					970:983	scaled-up implementation	960:983	scaled-up implementation	960:983	Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts.					
34315131	5	38	theme	Culture	768:774	arg1	conditions					776:785	Culture conditions	768:785	Culture conditions	768:785	Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers.					
34315131	3	39	theme	%	520:520	arg1	similarity					522:531	100% similarity	517:531	100% similarity to that of pediocin PA-1	517:556	Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1.					
34315131	3	40	with	operon	505:510	arg1	similarity					522:531	100% similarity	517:531	100% similarity to that of pediocin PA-1	517:556	Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1.					
34315131	7	41	theme	proper	1134:1139	arg1	valorisation					1159:1170	a future proper sugarcane bagasse valorisation	1125:1170	a future proper sugarcane bagasse valorisation for pediocin production	1125:1194	This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.					
34315131	4	42	theme	0.8 mg·L-1	724:733	arg1	yield					715:719	a yield	713:719	a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%)	713:765	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	4	43	theme	pediocin	743:750	arg1	0.8 mg·L-1					724:733	0.8 mg·L-1	724:733	0.8 mg·L-1 of pure pediocin (purity > 95%)	724:765	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	5	44	theme	central	817:823	arg1	design					835:840	a central composite design	815:840	a central composite design using temperature and hydrolysate % as independent variables	815:901	Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers.					
34315131	1	45	theme	Listeria	166:173	arg1	monocytogenes					175:187	Listeria monocytogenes	166:187	Listeria monocytogenes	166:187	Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety.					
34315131	6	46	theme	biorefinery	1057:1067	arg1	concepts					1069:1076	biorefinery concepts	1057:1076	biorefinery concepts	1057:1076	Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts.					
34315131	0	47	theme	hemicellulose	78:90	arg1	hydrolysate					92:102	non-detoxified hemicellulose hydrolysate	63:102	non-detoxified hemicellulose hydrolysate	63:102	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse.					
34315131	4	48	theme	purification	674:685	arg1	step					687:690	a non-optimized purification step	658:690	a non-optimized purification step sequence	658:699	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	4	49	theme	PA-1	643:646	arg1	genes					648:652	PA-1 genes	643:652	PA-1 genes	643:652	ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%).					
34315131	0	50	theme	non-detoxified	63:76	arg1	hydrolysate					92:102	non-detoxified hemicellulose hydrolysate	63:102	non-detoxified hemicellulose hydrolysate	63:102	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse.					
34315131	3	51	theme	pediocin	544:551	arg1	PA-1					553:556	pediocin PA-1	544:556	pediocin PA-1	544:556	Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1.					
33567643	0	0	theme	Synthesis	67:75	arg1	Inducer					46:52	an Inducer	43:52	an Inducer of Surfactin Synthesis by Bacillus subtilis	43:96	Xylan Decomposition in Plant Cell Walls as an Inducer of Surfactin Synthesis by Bacillus subtilis.					
33567643	5	1	theme	particular	731:740	arg1	xylan					742:746	particular xylan	731:746	particular xylan	731:746	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	1	2	theme	plant	141:145	arg1	biopolymer					160:169	the second most abundant plant heterogenous biopolymer	116:169	the second most abundant plant heterogenous biopolymer	116:169	Hemicellulose is the second most abundant plant heterogenous biopolymer.					
33567643	1	2	theme	plant	141:145	arg1	Hemicellulose					99:111	Hemicellulose	99:111	Hemicellulose	99:111	Hemicellulose is the second most abundant plant heterogenous biopolymer.					
33567643	4	3	theme	B.	557:558	arg1	87Y					569:571	B. subtilis 87Y	557:571	B. subtilis 87Y	557:571	This work aimed to investigate plant hemicellulose components as substrates promoting SU's efficient production by B. subtilis 87Y.					
33567643	0	4	theme	Surfactin	57:65	arg1	Synthesis					67:75	Surfactin Synthesis	57:75	Surfactin Synthesis	57:75	Xylan Decomposition in Plant Cell Walls as an Inducer of Surfactin Synthesis by Bacillus subtilis.					
33567643	0	5	from	Decomposition	6:18	arg1	Walls					34:38	Plant Cell Walls	23:38	Plant Cell Walls	23:38	Xylan Decomposition in Plant Cell Walls as an Inducer of Surfactin Synthesis by Bacillus subtilis.					
33567643	3	6	dep	Bacillus	342:349	arg1	subtilis					351:358	subtilis	351:358	subtilis	351:358	Our previous studies have shown that a Bacillus subtilis strain can successfully produce a significant amount of SU using a rapeseed cake.					
33567643	1	7	theme	heterogenous	147:158	arg1	biopolymer					160:169	the second most abundant plant heterogenous biopolymer	116:169	the second most abundant plant heterogenous biopolymer	116:169	Hemicellulose is the second most abundant plant heterogenous biopolymer.					
33567643	1	7	theme	heterogenous	147:158	arg1	Hemicellulose					99:111	Hemicellulose	99:111	Hemicellulose	99:111	Hemicellulose is the second most abundant plant heterogenous biopolymer.					
33567643	4	8	theme	plant	473:477	arg1	substrates					507:516	substrates	507:516	substrates promoting SU's efficient production by B. subtilis 87Y	507:571	This work aimed to investigate plant hemicellulose components as substrates promoting SU's efficient production by B. subtilis 87Y.					
33567643	4	8	theme	plant	473:477	arg1	components					493:502	plant hemicellulose components	473:502	plant hemicellulose components	473:502	This work aimed to investigate plant hemicellulose components as substrates promoting SU's efficient production by B. subtilis 87Y.					
33567643	5	9	theme	wall	629:632	arg1	composition					634:644	cell wall composition	624:644	cell wall composition	624:644	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	10	from	responsible	776:786	arg1	derivatives					756:766	its derivatives	752:766	its derivatives	752:766	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	10	from	responsible	776:786	arg1	xylan					742:746	particular xylan	731:746	particular xylan	731:746	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	11	theme	composition	634:644	arg1	Analyses					574:581	Analyses	574:581	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses	574:668	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	12	theme	plant	707:711	arg1	hemicellulose					713:725	plant hemicellulose	707:725	plant hemicellulose	707:725	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	13	theme	production	589:598	arg1	Analyses					574:581	Analyses	574:581	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses	574:668	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	14	theme	SU	586:587	arg1	production					589:598	SU production	586:598	SU production	586:598	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	15	theme	hemicellulose	713:725	arg1	ingredients					692:702	the main ingredients	683:702	the main ingredients of plant hemicellulose	683:725	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	15	theme	hemicellulose	713:725	arg1	responsible					776:786	responsible	776:786	responsible	776:786	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	3	16	theme	SU	416:417	arg1	SU					416:417	SU	416:417	SU	416:417	Our previous studies have shown that a Bacillus subtilis strain can successfully produce a significant amount of SU using a rapeseed cake.					
33567643	3	16	theme	SU	416:417	arg1	amount					406:411	a significant amount	392:411	a significant amount of SU	392:417	Our previous studies have shown that a Bacillus subtilis strain can successfully produce a significant amount of SU using a rapeseed cake.					
33567643	5	17	theme	enzymatic	601:609	arg1	activity					611:618	enzymatic activity	601:618	enzymatic activity	601:618	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	3	18	theme	significant	394:404	arg1	SU					416:417	SU	416:417	SU	416:417	Our previous studies have shown that a Bacillus subtilis strain can successfully produce a significant amount of SU using a rapeseed cake.					
33567643	3	18	theme	significant	394:404	arg1	amount					406:411	a significant amount	392:411	a significant amount of SU	392:417	Our previous studies have shown that a Bacillus subtilis strain can successfully produce a significant amount of SU using a rapeseed cake.					
33567643	4	19	theme	hemicellulose	479:491	arg1	substrates					507:516	substrates	507:516	substrates promoting SU's efficient production by B. subtilis 87Y	507:571	This work aimed to investigate plant hemicellulose components as substrates promoting SU's efficient production by B. subtilis 87Y.					
33567643	4	19	theme	hemicellulose	479:491	arg1	components					493:502	plant hemicellulose components	473:502	plant hemicellulose components	473:502	This work aimed to investigate plant hemicellulose components as substrates promoting SU's efficient production by B. subtilis 87Y.					
33567643	5	20	theme	activity	611:618	arg1	Analyses					574:581	Analyses	574:581	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses	574:668	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	21	theme	cell	624:627	arg1	composition					634:644	cell wall composition	624:644	cell wall composition	624:644	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	0	22	theme	Plant	23:27	arg1	Walls					34:38	Plant Cell Walls	23:38	Plant Cell Walls	23:38	Xylan Decomposition in Plant Cell Walls as an Inducer of Surfactin Synthesis by Bacillus subtilis.					
33567643	3	23	theme	rapeseed	427:434	arg1	cake					436:439	a rapeseed cake	425:439	a rapeseed cake	425:439	Our previous studies have shown that a Bacillus subtilis strain can successfully produce a significant amount of SU using a rapeseed cake.					
33567643	3	24	theme	Bacillus	342:349	arg1	strain					360:365	a Bacillus subtilis strain	340:365	a Bacillus subtilis strain	340:365	Our previous studies have shown that a Bacillus subtilis strain can successfully produce a significant amount of SU using a rapeseed cake.					
33567643	5	25	theme	increased	795:803	arg1	yield					819:823	an increased biosurfactant yield	792:823	an increased biosurfactant yield	792:823	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	4	26	theme	efficient	533:541	arg1	production					543:552	SU's efficient production	528:552	SU's efficient production by B. subtilis 87Y	528:571	This work aimed to investigate plant hemicellulose components as substrates promoting SU's efficient production by B. subtilis 87Y.					
33567643	5	27	theme	biosurfactant	805:817	arg1	yield					819:823	an increased biosurfactant yield	792:823	an increased biosurfactant yield	792:823	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	0	28	theme	Cell	29:32	arg1	Walls					34:38	Plant Cell Walls	23:38	Plant Cell Walls	23:38	Xylan Decomposition in Plant Cell Walls as an Inducer of Surfactin Synthesis by Bacillus subtilis.					
33567643	5	29	theme	main	687:690	arg1	ingredients					692:702	the main ingredients	683:702	the main ingredients of plant hemicellulose	683:725	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	29	theme	main	687:690	arg1	responsible					776:786	responsible	776:786	responsible	776:786	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	4	30	dep	B.	557:558	arg1	subtilis					560:567	subtilis	560:567	subtilis	560:567	This work aimed to investigate plant hemicellulose components as substrates promoting SU's efficient production by B. subtilis 87Y.					
33567643	2	31	theme	agro-residues	217:229	arg1	range					208:212	a wide range	201:212	a wide range of agro-residues	201:229	Among products obtained from a wide range of agro-residues, biosurfactants, e.g., surfactin (SU), are gaining increasing interest.					
33567643	5	32	theme	hulled	649:654	arg1	caryopses					660:668	hulled oat caryopses	649:668	hulled oat caryopses	649:668	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	2	33	theme	wide	203:206	arg1	range					208:212	a wide range	201:212	a wide range of agro-residues	201:229	Among products obtained from a wide range of agro-residues, biosurfactants, e.g., surfactin (SU), are gaining increasing interest.					
33567643	1	34	theme	abundant	132:139	arg1	biopolymer					160:169	the second most abundant plant heterogenous biopolymer	116:169	the second most abundant plant heterogenous biopolymer	116:169	Hemicellulose is the second most abundant plant heterogenous biopolymer.					
33567643	1	34	theme	abundant	132:139	arg1	Hemicellulose					99:111	Hemicellulose	99:111	Hemicellulose	99:111	Hemicellulose is the second most abundant plant heterogenous biopolymer.					
33567643	5	35	theme	oat	656:658	arg1	caryopses					660:668	hulled oat caryopses	649:668	hulled oat caryopses	649:668	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	36	from	xylan	742:746	arg1	ingredients					692:702	the main ingredients	683:702	the main ingredients of plant hemicellulose	683:725	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	36	from	xylan	742:746	arg1	responsible					776:786	responsible	776:786	responsible	776:786	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	37	theme	caryopses	660:668	arg1	production					589:598	SU production	586:598	SU production	586:598	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	37	theme	caryopses	660:668	arg1	activity					611:618	enzymatic activity	601:618	enzymatic activity	601:618	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	37	theme	caryopses	660:668	arg1	composition					634:644	cell wall composition	624:644	cell wall composition	624:644	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	1	38	theme	second	120:125	arg1	biopolymer					160:169	the second most abundant plant heterogenous biopolymer	116:169	the second most abundant plant heterogenous biopolymer	116:169	Hemicellulose is the second most abundant plant heterogenous biopolymer.					
33567643	1	38	theme	second	120:125	arg1	Hemicellulose					99:111	Hemicellulose	99:111	Hemicellulose	99:111	Hemicellulose is the second most abundant plant heterogenous biopolymer.					
33567643	3	39	theme	previous	307:314	arg1	studies					316:322	Our previous studies	303:322	Our previous studies	303:322	Our previous studies have shown that a Bacillus subtilis strain can successfully produce a significant amount of SU using a rapeseed cake.					
33567643	5	40	from	derivatives	756:766	arg1	ingredients					692:702	the main ingredients	683:702	the main ingredients of plant hemicellulose	683:725	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	5	40	from	derivatives	756:766	arg1	responsible					776:786	responsible	776:786	responsible	776:786	Analyses of SU production, enzymatic activity and cell wall composition of hulled oat caryopses suggest that the main ingredients of plant hemicellulose, in particular xylan and its derivatives, may be responsible for an increased biosurfactant yield.					
33567643	2	41	dep	surfactin	254:262	arg1	e.g.					248:251	e.g.	248:251	e.g.	248:251	Among products obtained from a wide range of agro-residues, biosurfactants, e.g., surfactin (SU), are gaining increasing interest.					
33743327	6	0	theme	heparin	910:916	arg1	analysis					882:889	a comprehensive analysis	866:889	a comprehensive analysis of enzyme digested heparin	866:916	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	1	theme	liquid	739:744	arg1	chromatography					746:759	a new two-dimensional liquid chromatography	717:759	a new two-dimensional liquid chromatography system	717:766	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	2	theme	digested	901:908	arg1	heparin					910:916	enzyme digested heparin	894:916	enzyme digested heparin	894:916	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	7	3	theme	dimensional	948:958	arg1	SAX					919:921	SAX	919:921	SAX	919:921	SAX was applied as the first dimensional chromatography, in which 17 peaks were observed and integrated in the digested heparin.					
33743327	7	3	theme	dimensional	948:958	arg1	chromatography					960:973	the first dimensional chromatography	938:973	the first dimensional chromatography	938:973	SAX was applied as the first dimensional chromatography, in which 17 peaks were observed and integrated in the digested heparin.					
33743327	9	4	theme	common	1241:1246	arg1	disaccharides					1248:1260	eight common disaccharides	1235:1260	eight common disaccharides	1235:1260	Structural information of each component was then obtained with MS, including eight common disaccharides, eight enzyme resistant tetrasaccharides and a heparin-core protein linkage domain.					
33743327	10	5	theme	digested	1371:1378	arg1	heparins					1380:1387	enzyme digested heparins	1364:1387	enzyme digested heparins obtained from different vendors using this system	1364:1437	The comparison of enzyme digested heparins obtained from different vendors using this system suggested their similar major structure and activity, but slightly different production processes.					
33743327	6	6	attach	linked	813:818	arg1	MS					842:843	MS	842:843	MS	842:843	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	6	attach	linked	813:818	arg2	cut					784:786	multiple heart cut	769:786	multiple heart cut (MHC)	769:792	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	6	attach	linked	813:818	arg1	spectrometry					828:839	mass spectrometry	823:839	mass spectrometry (MS)	823:844	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	6	attach	linked	813:818	arg2	system					761:766	a new two-dimensional liquid chromatography system	717:766	a new two-dimensional liquid chromatography system	717:766	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	7	theme	enzyme	894:899	arg1	heparin					910:916	enzyme digested heparin	894:916	enzyme digested heparin	894:916	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	10	8	theme	major	1463:1467	arg1	structure					1469:1477	their similar major structure	1449:1477	their similar major structure	1449:1477	The comparison of enzyme digested heparins obtained from different vendors using this system suggested their similar major structure and activity, but slightly different production processes.					
33743327	4	9	theme	exchange	426:433	arg1	chromatography					441:454	Strong anion exchange (SAX) chromatography	413:454	Strong anion exchange (SAX) chromatography	413:454	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	10	10	theme	enzyme	1364:1369	arg1	heparins					1380:1387	enzyme digested heparins	1364:1387	enzyme digested heparins obtained from different vendors using this system	1364:1437	The comparison of enzyme digested heparins obtained from different vendors using this system suggested their similar major structure and activity, but slightly different production processes.					
33743327	4	11	used	used	468:471	arg2	chromatography					441:454	Strong anion exchange (SAX) chromatography	413:454	Strong anion exchange (SAX) chromatography	413:454	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	9	12	theme	heparin-core	1309:1320	arg1	domain					1338:1343	a heparin-core protein linkage domain	1307:1343	a heparin-core protein linkage domain	1307:1343	Structural information of each component was then obtained with MS, including eight common disaccharides, eight enzyme resistant tetrasaccharides and a heparin-core protein linkage domain.					
33743327	10	13	theme	similar	1455:1461	arg1	structure					1469:1477	their similar major structure	1449:1477	their similar major structure	1449:1477	The comparison of enzyme digested heparins obtained from different vendors using this system suggested their similar major structure and activity, but slightly different production processes.					
33743327	9	14	theme	component	1188:1196	arg1	information					1168:1178	Structural information	1157:1178	Structural information of each component	1157:1196	Structural information of each component was then obtained with MS, including eight common disaccharides, eight enzyme resistant tetrasaccharides and a heparin-core protein linkage domain.					
33743327	6	15	theme	two-dimensional	723:737	arg1	chromatography					746:759	a new two-dimensional liquid chromatography	717:759	a new two-dimensional liquid chromatography system	717:766	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	8	16	theme	dimensional	1107:1117	arg1	chromatography					1119:1132	the second dimensional chromatography	1096:1132	the second dimensional chromatography	1096:1132	Size-exclusion chromatography (SEC) was used as the second dimensional chromatography to desalt efficiently.					
33743327	8	16	theme	dimensional	1107:1117	arg1	chromatography					1063:1076	Size-exclusion chromatography	1048:1076	Size-exclusion chromatography (SEC)	1048:1082	Size-exclusion chromatography (SEC) was used as the second dimensional chromatography to desalt efficiently.					
33743327	4	17	theme	due	504:506	arg1	analysis					495:502	the compositional analysis	477:502	the compositional analysis due to its high resolution, stability and capability of quantitation	477:571	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	4	18	theme	SAX	436:438	arg1	chromatography					441:454	Strong anion exchange (SAX) chromatography	413:454	Strong anion exchange (SAX) chromatography	413:454	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	6	19	theme	new	719:721	arg1	chromatography					746:759	a new two-dimensional liquid chromatography	717:759	a new two-dimensional liquid chromatography system	717:766	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	4	20	theme	high	515:518	arg1	resolution					520:529	its high resolution	511:529	its high resolution	511:529	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	9	21	theme	enzyme	1269:1274	arg1	tetrasaccharides					1286:1301	eight enzyme resistant tetrasaccharides	1263:1301	eight enzyme resistant tetrasaccharides	1263:1301	Structural information of each component was then obtained with MS, including eight common disaccharides, eight enzyme resistant tetrasaccharides and a heparin-core protein linkage domain.					
33743327	3	22	theme	compositional	266:278	arg1	analysis					280:287	The compositional analysis	262:287	The compositional analysis after heparin was decomposed to disaccharides and enzyme resistant domains	262:362	The compositional analysis after heparin was decomposed to disaccharides and enzyme resistant domains is an important way to delve into its structure.					
33743327	3	22	theme	compositional	266:278	arg1	way					380:382	an important way to delve into its structure	367:410	an important way to delve into its structure	367:410	The compositional analysis after heparin was decomposed to disaccharides and enzyme resistant domains is an important way to delve into its structure.					
33743327	5	23	theme	mobile	603:608	arg1	phase					610:614	mobile phase	603:614	mobile phase	603:614	However, nonvolatile salt in mobile phase is not compatible with MS, then the structural domains cannot be identified without standards.					
33743327	9	24	theme	protein	1322:1328	arg1	domain					1338:1343	a heparin-core protein linkage domain	1307:1343	a heparin-core protein linkage domain	1307:1343	Structural information of each component was then obtained with MS, including eight common disaccharides, eight enzyme resistant tetrasaccharides and a heparin-core protein linkage domain.					
33743327	0	25	theme	Comprehensive	0:12	arg1	analysis					14:21	Comprehensive analysis	0:21	Comprehensive analysis of heparinase	0:35	Comprehensive analysis of heparinase derived heparin-products using two-dimensional liquid chromatography coupled with mass spectrometry.					
33743327	5	26	theme	structural	652:661	arg1	domains					663:669	the structural domains	648:669	the structural domains	648:669	However, nonvolatile salt in mobile phase is not compatible with MS, then the structural domains cannot be identified without standards.					
33743327	4	27	theme	anion	420:424	arg1	chromatography					441:454	Strong anion exchange (SAX) chromatography	413:454	Strong anion exchange (SAX) chromatography	413:454	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	10	28	theme	different	1403:1411	arg1	vendors					1413:1419	different vendors	1403:1419	different vendors using this system	1403:1437	The comparison of enzyme digested heparins obtained from different vendors using this system suggested their similar major structure and activity, but slightly different production processes.					
33743327	0	29	theme	heparinase	26:35	arg1	analysis					14:21	Comprehensive analysis	0:21	Comprehensive analysis of heparinase	0:35	Comprehensive analysis of heparinase derived heparin-products using two-dimensional liquid chromatography coupled with mass spectrometry.					
33743327	4	30	theme	Strong	413:418	arg1	chromatography					441:454	Strong anion exchange (SAX) chromatography	413:454	Strong anion exchange (SAX) chromatography	413:454	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	6	31	theme	heart	778:782	arg1	system					761:766	a new two-dimensional liquid chromatography system	717:766	a new two-dimensional liquid chromatography system	717:766	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	31	theme	heart	778:782	arg1	MHC					789:791	MHC	789:791	MHC	789:791	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	31	theme	heart	778:782	arg1	cut					784:786	multiple heart cut	769:786	multiple heart cut (MHC)	769:792	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	2	32	theme	repeating	203:211	arg1	unit					226:229	a repeating disaccharide unit	201:229	a repeating disaccharide unit with different sulfo patterns	201:259	It is composed of a repeating disaccharide unit with different sulfo patterns.					
33743327	7	33	theme	first	942:946	arg1	SAX					919:921	SAX	919:921	SAX	919:921	SAX was applied as the first dimensional chromatography, in which 17 peaks were observed and integrated in the digested heparin.					
33743327	7	33	theme	first	942:946	arg1	chromatography					960:973	the first dimensional chromatography	938:973	the first dimensional chromatography	938:973	SAX was applied as the first dimensional chromatography, in which 17 peaks were observed and integrated in the digested heparin.					
33743327	5	34	from	salt	595:598	arg1	phase					610:614	mobile phase	603:614	mobile phase	603:614	However, nonvolatile salt in mobile phase is not compatible with MS, then the structural domains cannot be identified without standards.					
33743327	9	35	theme	linkage	1330:1336	arg1	domain					1338:1343	a heparin-core protein linkage domain	1307:1343	a heparin-core protein linkage domain	1307:1343	Structural information of each component was then obtained with MS, including eight common disaccharides, eight enzyme resistant tetrasaccharides and a heparin-core protein linkage domain.					
33743327	10	36	theme	production	1516:1525	arg1	processes					1527:1535	slightly different production processes	1497:1535	slightly different production processes	1497:1535	The comparison of enzyme digested heparins obtained from different vendors using this system suggested their similar major structure and activity, but slightly different production processes.					
33743327	10	37	theme	different	1506:1514	arg1	processes					1527:1535	slightly different production processes	1497:1535	slightly different production processes	1497:1535	The comparison of enzyme digested heparins obtained from different vendors using this system suggested their similar major structure and activity, but slightly different production processes.					
33743327	5	38	with	compatible	623:632	arg1	MS					639:640	MS	639:640	MS	639:640	However, nonvolatile salt in mobile phase is not compatible with MS, then the structural domains cannot be identified without standards.					
33743327	0	39	theme	two-dimensional	68:82	arg1	chromatography					91:104	two-dimensional liquid chromatography	68:104	two-dimensional liquid chromatography coupled with mass spectrometry	68:135	Comprehensive analysis of heparinase derived heparin-products using two-dimensional liquid chromatography coupled with mass spectrometry.					
33743327	3	40	theme	resistant	346:354	arg1	domains					356:362	enzyme resistant domains	339:362	enzyme resistant domains	339:362	The compositional analysis after heparin was decomposed to disaccharides and enzyme resistant domains is an important way to delve into its structure.					
33743327	4	41	theme	compositional	481:493	arg1	analysis					495:502	the compositional analysis	477:502	the compositional analysis due to its high resolution, stability and capability of quantitation	477:571	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	6	42	theme	comprehensive	868:880	arg1	analysis					882:889	a comprehensive analysis	866:889	a comprehensive analysis of enzyme digested heparin	866:916	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	7	43	theme	digested	1030:1037	arg1	heparin					1039:1045	the digested heparin	1026:1045	the digested heparin	1026:1045	SAX was applied as the first dimensional chromatography, in which 17 peaks were observed and integrated in the digested heparin.					
33743327	2	44	theme	different	236:244	arg1	patterns					252:259	different sulfo patterns	236:259	different sulfo patterns	236:259	It is composed of a repeating disaccharide unit with different sulfo patterns.					
33743327	2	45	theme	sulfo	246:250	arg1	patterns					252:259	different sulfo patterns	236:259	different sulfo patterns	236:259	It is composed of a repeating disaccharide unit with different sulfo patterns.					
33743327	5	46	theme	nonvolatile	583:593	arg1	salt					595:598	nonvolatile salt	583:598	nonvolatile salt in mobile phase	583:614	However, nonvolatile salt in mobile phase is not compatible with MS, then the structural domains cannot be identified without standards.					
33743327	8	47	theme	Size-exclusion	1048:1061	arg1	SEC					1079:1081	SEC	1079:1081	SEC	1079:1081	Size-exclusion chromatography (SEC) was used as the second dimensional chromatography to desalt efficiently.					
33743327	8	47	theme	Size-exclusion	1048:1061	arg1	chromatography					1119:1132	the second dimensional chromatography	1096:1132	the second dimensional chromatography	1096:1132	Size-exclusion chromatography (SEC) was used as the second dimensional chromatography to desalt efficiently.					
33743327	8	47	theme	Size-exclusion	1048:1061	arg1	chromatography					1063:1076	Size-exclusion chromatography	1048:1076	Size-exclusion chromatography (SEC)	1048:1082	Size-exclusion chromatography (SEC) was used as the second dimensional chromatography to desalt efficiently.					
33743327	4	48	theme	quantitation	560:571	arg1	capability					546:555	capability	546:555	capability	546:555	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	4	48	theme	quantitation	560:571	arg1	stability					532:540	stability	532:540	stability	532:540	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	4	48	theme	quantitation	560:571	arg1	resolution					520:529	its high resolution	511:529	its high resolution	511:529	Strong anion exchange (SAX) chromatography is commonly used for the compositional analysis due to its high resolution, stability and capability of quantitation.					
33743327	8	49	theme	second	1100:1105	arg1	chromatography					1119:1132	the second dimensional chromatography	1096:1132	the second dimensional chromatography	1096:1132	Size-exclusion chromatography (SEC) was used as the second dimensional chromatography to desalt efficiently.					
33743327	8	49	theme	second	1100:1105	arg1	chromatography					1063:1076	Size-exclusion chromatography	1048:1076	Size-exclusion chromatography (SEC)	1048:1082	Size-exclusion chromatography (SEC) was used as the second dimensional chromatography to desalt efficiently.					
33743327	6	50	theme	multiple	769:776	arg1	system					761:766	a new two-dimensional liquid chromatography system	717:766	a new two-dimensional liquid chromatography system	717:766	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	50	theme	multiple	769:776	arg1	MHC					789:791	MHC	789:791	MHC	789:791	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	50	theme	multiple	769:776	arg1	cut					784:786	multiple heart cut	769:786	multiple heart cut (MHC)	769:792	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	1	51	theme	linear	151:156	arg1	polysaccharide					167:180	a linear sulfated polysaccharide	149:180	a linear sulfated polysaccharide	149:180	Heparin is a linear sulfated polysaccharide.					
33743327	1	51	theme	linear	151:156	arg1	Heparin					138:144	Heparin	138:144	Heparin	138:144	Heparin is a linear sulfated polysaccharide.					
33743327	9	52	theme	Structural	1157:1166	arg1	information					1168:1178	Structural information	1157:1178	Structural information of each component	1157:1196	Structural information of each component was then obtained with MS, including eight common disaccharides, eight enzyme resistant tetrasaccharides and a heparin-core protein linkage domain.					
33743327	3	53	theme	enzyme	339:344	arg1	domains					356:362	enzyme resistant domains	339:362	enzyme resistant domains	339:362	The compositional analysis after heparin was decomposed to disaccharides and enzyme resistant domains is an important way to delve into its structure.					
33743327	7	54	located	observed	999:1006	arg1	SAX					919:921	SAX	919:921	SAX	919:921	SAX was applied as the first dimensional chromatography, in which 17 peaks were observed and integrated in the digested heparin.					
33743327	7	54	located	observed	999:1006	arg2	peaks					988:992	17 peaks	985:992	17 peaks	985:992	SAX was applied as the first dimensional chromatography, in which 17 peaks were observed and integrated in the digested heparin.					
33743327	7	54	located	observed	999:1006	arg1	chromatography					960:973	the first dimensional chromatography	938:973	the first dimensional chromatography	938:973	SAX was applied as the first dimensional chromatography, in which 17 peaks were observed and integrated in the digested heparin.					
33743327	1	55	theme	sulfated	158:165	arg1	polysaccharide					167:180	a linear sulfated polysaccharide	149:180	a linear sulfated polysaccharide	149:180	Heparin is a linear sulfated polysaccharide.					
33743327	1	55	theme	sulfated	158:165	arg1	Heparin					138:144	Heparin	138:144	Heparin	138:144	Heparin is a linear sulfated polysaccharide.					
33743327	3	56	theme	important	370:378	arg1	analysis					280:287	The compositional analysis	262:287	The compositional analysis after heparin was decomposed to disaccharides and enzyme resistant domains	262:362	The compositional analysis after heparin was decomposed to disaccharides and enzyme resistant domains is an important way to delve into its structure.					
33743327	3	56	theme	important	370:378	arg1	way					380:382	an important way to delve into its structure	367:410	an important way to delve into its structure	367:410	The compositional analysis after heparin was decomposed to disaccharides and enzyme resistant domains is an important way to delve into its structure.					
33743327	2	57	theme	disaccharide	213:224	arg1	unit					226:229	a repeating disaccharide unit	201:229	a repeating disaccharide unit with different sulfo patterns	201:259	It is composed of a repeating disaccharide unit with different sulfo patterns.					
33743327	8	58	used	used	1088:1091	arg2	chromatography					1063:1076	Size-exclusion chromatography	1048:1076	Size-exclusion chromatography (SEC)	1048:1082	Size-exclusion chromatography (SEC) was used as the second dimensional chromatography to desalt efficiently.					
33743327	8	58	used	used	1088:1091	arg2	chromatography					1119:1132	the second dimensional chromatography	1096:1132	the second dimensional chromatography	1096:1132	Size-exclusion chromatography (SEC) was used as the second dimensional chromatography to desalt efficiently.					
33743327	8	58	used	used	1088:1091	arg2	SEC					1079:1081	SEC	1079:1081	SEC	1079:1081	Size-exclusion chromatography (SEC) was used as the second dimensional chromatography to desalt efficiently.					
33743327	10	59	theme	heparins	1380:1387	arg1	comparison					1350:1359	The comparison	1346:1359	The comparison of enzyme digested heparins obtained from different vendors using this system	1346:1437	The comparison of enzyme digested heparins obtained from different vendors using this system suggested their similar major structure and activity, but slightly different production processes.					
33743327	2	60	with	unit	226:229	arg1	patterns					252:259	different sulfo patterns	236:259	different sulfo patterns	236:259	It is composed of a repeating disaccharide unit with different sulfo patterns.					
33743327	6	61	theme	mass	823:826	arg1	MS					842:843	MS	842:843	MS	842:843	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	61	theme	mass	823:826	arg1	spectrometry					828:839	mass spectrometry	823:839	mass spectrometry (MS)	823:844	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	0	62	theme	liquid	84:89	arg1	chromatography					91:104	two-dimensional liquid chromatography	68:104	two-dimensional liquid chromatography coupled with mass spectrometry	68:135	Comprehensive analysis of heparinase derived heparin-products using two-dimensional liquid chromatography coupled with mass spectrometry.					
33743327	9	63	theme	resistant	1276:1284	arg1	tetrasaccharides					1286:1301	eight enzyme resistant tetrasaccharides	1263:1301	eight enzyme resistant tetrasaccharides	1263:1301	Structural information of each component was then obtained with MS, including eight common disaccharides, eight enzyme resistant tetrasaccharides and a heparin-core protein linkage domain.					
33743327	6	64	theme	chromatography	746:759	arg1	system					761:766	a new two-dimensional liquid chromatography system	717:766	a new two-dimensional liquid chromatography system	717:766	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	6	64	theme	chromatography	746:759	arg1	cut					784:786	multiple heart cut	769:786	multiple heart cut (MHC)	769:792	Here, a new two-dimensional liquid chromatography system, multiple heart cut (MHC), was developed and linked to mass spectrometry (MS) directly to provide a comprehensive analysis of enzyme digested heparin.					
33743327	0	65	theme	mass	119:122	arg1	spectrometry					124:135	mass spectrometry	119:135	mass spectrometry	119:135	Comprehensive analysis of heparinase derived heparin-products using two-dimensional liquid chromatography coupled with mass spectrometry.					
33594885	4	0	theme	More	754:757	arg1	associations					759:770	More associations	754:770	More associations of HCS/KGM	754:781	More associations of HCS/KGM enhanced the mechanical properties and reduced the moisture content of the films.					
33594885	2	1	theme	Fourier	408:414	arg1	results					349:355	The results	345:355	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy	345:508	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	1	2	theme	structural	296:305	arg1	properties					333:342	structural, mechanical, and physical properties	296:342	structural, mechanical, and physical properties	296:342	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	2	3	theme	analyses	467:474	arg1	results					349:355	The results	345:355	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy	345:508	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	2	4	theme	reflectance	396:406	arg1	Fourier					408:414	attenuated total reflectance Fourier	379:414	attenuated total reflectance Fourier transform infrared spectroscopy	379:446	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	2	5	theme	film	602:605	arg1	formation					607:615	film formation	602:615	film formation	602:615	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	2	6	dep	Fourier	408:414	arg1	transform					416:424	transform	416:424	transform infrared spectroscopy	416:446	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	1	7	theme	mechanical	308:317	arg1	properties					333:342	structural, mechanical, and physical properties	296:342	structural, mechanical, and physical properties	296:342	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	2	8	theme	X-ray	360:364	arg1	diffraction					366:376	X-ray diffraction	360:376	X-ray diffraction	360:376	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	4	9	theme	moisture	834:841	arg1	content					843:849	the moisture content	830:849	the moisture content of the films	830:862	More associations of HCS/KGM enhanced the mechanical properties and reduced the moisture content of the films.					
33594885	2	10	theme	total	390:394	arg1	Fourier					408:414	attenuated total reflectance Fourier	379:414	attenuated total reflectance Fourier transform infrared spectroscopy	379:446	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	5	11	theme	composite	909:917	arg1	film					919:922	the HCS/KGM composite film	897:922	the HCS/KGM composite film	897:922	The water vapor permeability of the HCS/KGM composite film was also improved significantly with the incorporation of β-CD.					
33594885	1	12	theme	various	213:219	arg1	concentrations					221:234	various concentrations	213:234	various concentrations of β-cyclodextrin (β-CD)	213:259	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	5	13	theme	β-CD	982:985	arg1	incorporation					965:977	the incorporation	961:977	the incorporation of β-CD	961:985	The water vapor permeability of the HCS/KGM composite film was also improved significantly with the incorporation of β-CD.					
33594885	2	14	dep	transform	416:424	arg1	infrared					426:433	infrared	426:433	transform infrared spectroscopy	416:446	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	2	15	theme	thermogravimetric	449:465	arg1	analyses					467:474	thermogravimetric analyses	449:474	thermogravimetric analyses	449:474	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	0	16	theme	Corn	13:16	arg1	Film					54:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film: Reinforced by Incorporating β-Cyclodextrin.					
33594885	1	17	theme	Glycerol-plasticized	104:123	arg1	HCS/KGM					170:176	HCS/KGM	170:176	HCS/KGM	170:176	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	1	17	theme	Glycerol-plasticized	104:123	arg1	glucomannan					157:167	Glycerol-plasticized high-amylose corn starch/konjac glucomannan	104:167	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD)	104:259	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	0	18	theme	High-Amylose	0:11	arg1	Film					54:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film: Reinforced by Incorporating β-Cyclodextrin.					
33594885	1	19	theme	high-amylose	125:136	arg1	HCS/KGM					170:176	HCS/KGM	170:176	HCS/KGM	170:176	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	1	19	theme	high-amylose	125:136	arg1	glucomannan					157:167	Glycerol-plasticized high-amylose corn starch/konjac glucomannan	104:167	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD)	104:259	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	1	20	theme	β-cyclodextrin	239:252	arg1	concentrations					221:234	various concentrations	213:234	various concentrations of β-cyclodextrin (β-CD)	213:259	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	0	21	theme	Glucomannan	32:42	arg1	Film					54:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film: Reinforced by Incorporating β-Cyclodextrin.					
33594885	1	22	theme	physical	324:331	arg1	properties					333:342	structural, mechanical, and physical properties	296:342	structural, mechanical, and physical properties	296:342	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	1	23	theme	corn	138:141	arg1	HCS/KGM					170:176	HCS/KGM	170:176	HCS/KGM	170:176	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	1	23	theme	corn	138:141	arg1	glucomannan					157:167	Glycerol-plasticized high-amylose corn starch/konjac glucomannan	104:167	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD)	104:259	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	2	24	theme	electron	490:497	arg1	microscopy					499:508	scanning electron microscopy	481:508	scanning electron microscopy	481:508	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	0	25	theme	Starch/Konjac	18:30	arg1	Film					54:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film: Reinforced by Incorporating β-Cyclodextrin.					
33594885	3	26	theme	films	709:713	arg1	thickness					675:683	thickness	675:683	thickness	675:683	The thickness and transparency of the films increased after β-CD was incorporated.					
33594885	3	26	theme	films	709:713	arg1	transparency					689:700	transparency	689:700	transparency	689:700	The thickness and transparency of the films increased after β-CD was incorporated.					
33594885	1	27	theme	starch/konjac	143:155	arg1	HCS/KGM					170:176	HCS/KGM	170:176	HCS/KGM	170:176	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	1	27	theme	starch/konjac	143:155	arg1	glucomannan					157:167	Glycerol-plasticized high-amylose corn starch/konjac glucomannan	104:167	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD)	104:259	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	6	28	theme	enhanced	992:999	arg1	association					1001:1011	The enhanced association	988:1011	The enhanced association between biopolymers in the presence of β-CD	988:1055	The enhanced association between biopolymers in the presence of β-CD will advance the development of a degradable active composite packaging film.					
33594885	2	29	theme	scanning	481:488	arg1	microscopy					499:508	scanning electron microscopy	481:508	scanning electron microscopy	481:508	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	2	30	theme	polymer	548:554	arg1	chains					556:561	the polymer chains	544:561	the polymer chains	544:561	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	4	31	theme	HCS/KGM	775:781	arg1	associations					759:770	More associations	754:770	More associations of HCS/KGM	754:781	More associations of HCS/KGM enhanced the mechanical properties and reduced the moisture content of the films.					
33594885	6	32	theme	film	1129:1132	arg1	development					1074:1084	the development	1070:1084	the development of a degradable active composite packaging film	1070:1132	The enhanced association between biopolymers in the presence of β-CD will advance the development of a degradable active composite packaging film.					
33594885	0	33	dep	Reinforced	60:69	arg1	Film					54:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film: Reinforced by Incorporating β-Cyclodextrin.					
33594885	5	34	theme	film	919:922	arg1	permeability					881:892	The water vapor permeability	865:892	The water vapor permeability of the HCS/KGM composite film	865:922	The water vapor permeability of the HCS/KGM composite film was also improved significantly with the incorporation of β-CD.					
33594885	0	35	theme	Composite	44:52	arg1	Film					54:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film	0:57	High-Amylose Corn Starch/Konjac Glucomannan Composite Film: Reinforced by Incorporating β-Cyclodextrin.					
33594885	6	36	theme	packaging	1119:1127	arg1	film					1129:1132	a degradable active composite packaging film	1089:1132	a degradable active composite packaging film	1089:1132	The enhanced association between biopolymers in the presence of β-CD will advance the development of a degradable active composite packaging film.					
33594885	6	37	from	biopolymers	1021:1031	arg1	presence					1040:1047	the presence	1036:1047	the presence of β-CD	1036:1055	The enhanced association between biopolymers in the presence of β-CD will advance the development of a degradable active composite packaging film.					
33594885	6	38	theme	β-CD	1052:1055	arg1	presence					1040:1047	the presence	1036:1047	the presence of β-CD	1036:1055	The enhanced association between biopolymers in the presence of β-CD will advance the development of a degradable active composite packaging film.					
33594885	1	39	theme	glucomannan	157:167	arg1	films					189:193	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films	104:193	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD)	104:259	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	5	40	theme	water	869:873	arg1	permeability					881:892	The water vapor permeability	865:892	The water vapor permeability of the HCS/KGM composite film	865:922	The water vapor permeability of the HCS/KGM composite film was also improved significantly with the incorporation of β-CD.					
33594885	2	41	theme	attenuated	379:388	arg1	Fourier					408:414	attenuated total reflectance Fourier	379:414	attenuated total reflectance Fourier transform infrared spectroscopy	379:446	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	4	42	theme	mechanical	796:805	arg1	properties					807:816	the mechanical properties	792:816	the mechanical properties	792:816	More associations of HCS/KGM enhanced the mechanical properties and reduced the moisture content of the films.					
33594885	6	43	theme	composite	1109:1117	arg1	film					1129:1132	a degradable active composite packaging film	1089:1132	a degradable active composite packaging film	1089:1132	The enhanced association between biopolymers in the presence of β-CD will advance the development of a degradable active composite packaging film.					
33594885	4	44	theme	films	858:862	arg1	content					843:849	the moisture content	830:849	the moisture content of the films	830:862	More associations of HCS/KGM enhanced the mechanical properties and reduced the moisture content of the films.					
33594885	2	45	theme	diffraction	366:376	arg1	results					349:355	The results	345:355	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy	345:508	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	6	46	theme	active	1102:1107	arg1	film					1129:1132	a degradable active composite packaging film	1089:1132	a degradable active composite packaging film	1089:1132	The enhanced association between biopolymers in the presence of β-CD will advance the development of a degradable active composite packaging film.					
33594885	5	47	theme	HCS/KGM	901:907	arg1	film					919:922	the HCS/KGM composite film	897:922	the HCS/KGM composite film	897:922	The water vapor permeability of the HCS/KGM composite film was also improved significantly with the incorporation of β-CD.					
33594885	1	48	theme	composite	179:187	arg1	films					189:193	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films	104:193	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD)	104:259	Glycerol-plasticized high-amylose corn starch/konjac glucomannan (HCS/KGM) composite films incorporated with various concentrations of β-cyclodextrin (β-CD) were prepared and investigated for structural, mechanical, and physical properties.					
33594885	6	49	theme	degradable	1091:1100	arg1	film					1129:1132	a degradable active composite packaging film	1089:1132	a degradable active composite packaging film	1089:1132	The enhanced association between biopolymers in the presence of β-CD will advance the development of a degradable active composite packaging film.					
33594885	2	50	theme	microscopy	499:508	arg1	results					349:355	The results	345:355	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy	345:508	The results of X-ray diffraction, attenuated total reflectance Fourier transform infrared spectroscopy, thermogravimetric analyses, and scanning electron microscopy indicated that β-CD excluded from the polymer chains and aggregated to form crystals during film formation, which drove HCS to interact with KGM more compactly.					
33594885	5	51	theme	vapor	875:879	arg1	permeability					881:892	The water vapor permeability	865:892	The water vapor permeability of the HCS/KGM composite film	865:922	The water vapor permeability of the HCS/KGM composite film was also improved significantly with the incorporation of β-CD.					
32576670	7	0	theme	human	1565:1569	arg1	gut					1571:1573	The human gut	1561:1573	The human gut	1561:1573	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	11	1	theme	thetaiotaomicron	2167:2182	arg1	layers					2243:2248	eight surface-exposed polysaccharidic layers	2205:2248	eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment	2205:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	1	theme	thetaiotaomicron	2167:2182	arg1	capsules					2184:2191	B. thetaiotaomicron capsules	2164:2191	B. thetaiotaomicron capsules	2164:2191	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	1	theme	thetaiotaomicron	2167:2182	arg1	group					2196:2200	a group	2194:2200	a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment	2194:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	1	theme	thetaiotaomicron	2167:2182	arg1	determinants					2324:2335	major determinants	2318:2335	major determinants of biofilm formation that mask or unmask adhesion factors	2318:2393	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	2	with	interactions	2270:2281	arg1	environment					2296:2306	the gut environment	2288:2306	the gut environment	2288:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	3	3	theme	capsules	735:742	arg1	one					698:700	one	698:700	one	698:700	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	3	3	theme	capsules	735:742	arg1	capsules					735:742	the eight B. thetaiotaomicron capsules	705:742	the eight B. thetaiotaomicron capsules	705:742	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	3	3	theme	capsules	735:742	arg1	capsule					687:693	capsule 4	687:695	capsule 4	687:695	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	3	4	theme	biofilm-related	550:564	arg1	functions					566:574	B. thetaiotaomicron biofilm-related functions	530:574	B. thetaiotaomicron biofilm-related functions	530:574	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	7	5	theme	thetaiotaomicron	1479:1494	arg1	structures					1504:1513	different B. thetaiotaomicron surface structures	1466:1513	different B. thetaiotaomicron surface structures	1466:1513	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	9	6	theme	adhesion	1954:1961	arg1	capacity					1963:1970	the adhesion capacity	1950:1970	the adhesion capacity of most gut bacteria	1950:1991	However, little is known about the adhesion capacity of most gut bacteria.					
32576670	3	7	theme	transposon	592:601	arg1	mutagenesis					603:613	a transposon mutagenesis	590:613	a transposon mutagenesis	590:613	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	6	8	theme	thetaiotaomicron	1177:1192	arg1	locus					1208:1212	the B. thetaiotaomicron glycosylation locus	1170:1212	the B. thetaiotaomicron glycosylation locus	1170:1212	Finally, we demonstrated that BT2934, the wzx homolog of the B. thetaiotaomicron glycosylation locus, competes with capsule production and impacts its adhesion capacity.					
32576670	7	9	dep	providing	1420:1428	arg1	harbors					1575:1581	harbors	1575:1581	harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections	1575:1760	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	9	10	theme	gut	1980:1982	arg1	bacteria					1984:1991	most gut bacteria	1975:1991	most gut bacteria	1975:1991	However, little is known about the adhesion capacity of most gut bacteria.					
32576670	6	11	theme	adhesion	1264:1271	arg1	capacity					1273:1280	its adhesion capacity	1260:1280	its adhesion capacity	1260:1280	Finally, we demonstrated that BT2934, the wzx homolog of the B. thetaiotaomicron glycosylation locus, competes with capsule production and impacts its adhesion capacity.					
32576670	7	12	theme	immune	1718:1723	arg1	system					1725:1730	the immune system	1714:1730	the immune system	1714:1730	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	4	13	theme	increased	934:942	arg1	formation					952:960	increased biofilm formation	934:960	increased biofilm formation	934:960	We then showed that the production of capsules 1, 2, 3, 5, and 6 also inhibits biofilm formation and that decreased capsulation of the population correlated with increased biofilm formation, suggesting that capsules could be masking adhesive surface structures.					
32576670	11	14	theme	adhesion	2378:2385	arg1	factors					2387:2393	adhesion factors	2378:2393	adhesion factors	2378:2393	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	5	15	theme	intrinsic	1082:1090	arg1	properties					1101:1110	intrinsic adhesive properties	1082:1110	intrinsic adhesive properties	1082:1110	In contrast, we showed that capsule 8 displayed intrinsic adhesive properties.					
32576670	12	16	theme	B.	2409:2410	arg1	thetaiotaomicron					2412:2427	B. thetaiotaomicron	2409:2427	B. thetaiotaomicron	2409:2427	Studying how B. thetaiotaomicron regulates its adhesion properties will allow us to better understand the physiology and specific properties of this important gut symbiont within anaerobic biofilms.					
32576670	7	17	theme	new	1430:1432	arg1	insights					1434:1441	new insights	1430:1441	new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE	1430:1559	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	10	18	theme	Bacteroides	2046:2056	arg1	one					2076:2078	one	2076:2078	one	2076:2078	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	10	18	theme	Bacteroides	2046:2056	arg1	thetaiotaomicron					2058:2073	Bacteroides thetaiotaomicron	2046:2073	Bacteroides thetaiotaomicron	2046:2073	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	10	18	theme	Bacteroides	2046:2056	arg1	bacteria					2101:2108	the most abundant bacteria	2083:2108	the most abundant bacteria of the normal mammalian intestine	2083:2142	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	1	19	theme	dietary	243:249	arg1	polysaccharides					251:265	dietary polysaccharides	243:265	dietary polysaccharides	243:265	Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny.					
32576670	3	20	theme	reference	645:653	arg1	strain					655:660	the poorly biofilm-forming reference strain VPI-5482	618:669	the poorly biofilm-forming reference strain VPI-5482	618:669	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	1	21	theme	gut	156:158	arg1	species					169:175	the most abundant gut symbiont species	138:175	the most abundant gut symbiont species	138:175	Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny.					
32576670	7	22	theme	nutrient	1678:1685	arg1	acquisition					1687:1697	nutrient acquisition	1678:1697	nutrient acquisition	1678:1697	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	6	23	theme	capsule	1229:1235	arg1	production					1237:1246	capsule production	1229:1246	capsule production	1229:1246	Finally, we demonstrated that BT2934, the wzx homolog of the B. thetaiotaomicron glycosylation locus, competes with capsule production and impacts its adhesion capacity.					
32576670	7	24	theme	biofilm	1401:1407	arg1	formation					1409:1417	B. thetaiotaomicron biofilm formation	1381:1417	B. thetaiotaomicron biofilm formation	1381:1417	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	6	25	theme	locus	1208:1212	arg1	BT2934					1143:1148	BT2934	1143:1148	BT2934	1143:1148	Finally, we demonstrated that BT2934, the wzx homolog of the B. thetaiotaomicron glycosylation locus, competes with capsule production and impacts its adhesion capacity.					
32576670	6	25	theme	locus	1208:1212	arg1	homolog					1159:1165	the wzx homolog	1151:1165	the wzx homolog of the B. thetaiotaomicron glycosylation locus	1151:1212	Finally, we demonstrated that BT2934, the wzx homolog of the B. thetaiotaomicron glycosylation locus, competes with capsule production and impacts its adhesion capacity.					
32576670	1	26	theme	species	169:175	arg1	species					169:175	the most abundant gut symbiont species	138:175	the most abundant gut symbiont species	138:175	Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny.					
32576670	1	26	theme	species	169:175	arg1	one					131:133	one	131:133	one	131:133	Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny.					
32576670	10	27	theme	intestine	2134:2142	arg1	bacteria					2101:2108	the most abundant bacteria	2083:2108	the most abundant bacteria of the normal mammalian intestine	2083:2142	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	7	28	theme	B.	1381:1382	arg1	formation					1409:1417	B. thetaiotaomicron biofilm formation	1381:1417	B. thetaiotaomicron biofilm formation	1381:1417	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	4	29	theme	adhesive	1005:1012	arg1	structures					1022:1031	masking adhesive surface structures	997:1031	masking adhesive surface structures	997:1031	We then showed that the production of capsules 1, 2, 3, 5, and 6 also inhibits biofilm formation and that decreased capsulation of the population correlated with increased biofilm formation, suggesting that capsules could be masking adhesive surface structures.					
32576670	10	30	theme	bacteria	2101:2108	arg1	one					2076:2078	one	2076:2078	one	2076:2078	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	10	30	theme	bacteria	2101:2108	arg1	thetaiotaomicron					2058:2073	Bacteroides thetaiotaomicron	2046:2073	Bacteroides thetaiotaomicron	2046:2073	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	10	30	theme	bacteria	2101:2108	arg1	bacteria					2101:2108	the most abundant bacteria	2083:2108	the most abundant bacteria of the normal mammalian intestine	2083:2142	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	12	31	dep	physiology	2502:2511	arg1	the					2498:2500	the	2498:2500	the	2498:2500	Studying how B. thetaiotaomicron regulates its adhesion properties will allow us to better understand the physiology and specific properties of this important gut symbiont within anaerobic biofilms.					
32576670	11	32	theme	B.	2164:2165	arg1	layers					2243:2248	eight surface-exposed polysaccharidic layers	2205:2248	eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment	2205:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	32	theme	B.	2164:2165	arg1	capsules					2184:2191	B. thetaiotaomicron capsules	2164:2191	B. thetaiotaomicron capsules	2164:2191	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	32	theme	B.	2164:2165	arg1	group					2196:2200	a group	2194:2200	a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment	2194:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	32	theme	B.	2164:2165	arg1	determinants					2324:2335	major determinants	2318:2335	major determinants of biofilm formation that mask or unmask adhesion factors	2318:2393	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	7	33	theme	host	1643:1646	arg1	health					1648:1653	host health	1643:1653	host health	1643:1653	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	11	34	theme	major	2318:2322	arg1	capsules					2184:2191	B. thetaiotaomicron capsules	2164:2191	B. thetaiotaomicron capsules	2164:2191	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	34	theme	major	2318:2322	arg1	determinants					2324:2335	major determinants	2318:2335	major determinants of biofilm formation that mask or unmask adhesion factors	2318:2393	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	4	35	theme	capsules	810:817	arg1	production					796:805	the production	792:805	the production of capsules 1, 2, 3, 5, and 6	792:835	We then showed that the production of capsules 1, 2, 3, 5, and 6 also inhibits biofilm formation and that decreased capsulation of the population correlated with increased biofilm formation, suggesting that capsules could be masking adhesive surface structures.					
32576670	6	36	theme	glycosylation	1194:1206	arg1	locus					1208:1212	the B. thetaiotaomicron glycosylation locus	1170:1212	the B. thetaiotaomicron glycosylation locus	1170:1212	Finally, we demonstrated that BT2934, the wzx homolog of the B. thetaiotaomicron glycosylation locus, competes with capsule production and impacts its adhesion capacity.					
32576670	1	37	theme	Bacteroides	99:109	arg1	thetaiotaomicron					111:126	Bacteroides thetaiotaomicron	99:126	Bacteroides thetaiotaomicron	99:126	Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny.					
32576670	8	38	dep	called	1819:1824	arg1	communities					1807:1817	communities	1807:1817	communities	1807:1817	The capacity to adhere to surfaces and form communities called biofilms is believed to be important for niche colonization and maintenance of gut bacteria.					
32576670	2	39	theme	microbiota	423:432	arg1	structure					434:442	microbiota structure	423:442	microbiota structure	423:442	In contrast, adhesion and biofilm formation, which are potentially involved in gut colonization and microbiota structure and stability, have hardly been investigated in this intestinal bacterium.					
32576670	8	40	theme	gut	1905:1907	arg1	bacteria					1909:1916	gut bacteria	1905:1916	gut bacteria	1905:1916	The capacity to adhere to surfaces and form communities called biofilms is believed to be important for niche colonization and maintenance of gut bacteria.					
32576670	11	41	theme	polysaccharidic	2227:2241	arg1	layers					2243:2248	eight surface-exposed polysaccharidic layers	2205:2248	eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment	2205:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	0	42	theme	Capsular	0:7	arg1	Cross-Regulation					24:39	Capsular Polysaccharide Cross-Regulation	0:39	Capsular Polysaccharide Cross-Regulation	0:39	Capsular Polysaccharide Cross-Regulation Modulates Bacteroides thetaiotaomicron Biofilm Formation.					
32576670	1	43	theme	intense	305:311	arg1	scrutiny					313:320	intense scrutiny	305:320	intense scrutiny	305:320	Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny.					
32576670	7	44	theme	major	1360:1364	arg1	regulation					1344:1353	B. thetaiotaomicron capsule regulation	1316:1353	B. thetaiotaomicron capsule regulation	1316:1353	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	44	theme	major	1360:1364	arg1	determinant					1366:1376	a major determinant	1358:1376	a major determinant of B. thetaiotaomicron biofilm formation	1358:1417	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	45	theme	different	1466:1474	arg1	structures					1504:1513	different B. thetaiotaomicron surface structures	1466:1513	different B. thetaiotaomicron surface structures	1466:1513	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	46	theme	system	1725:1730	arg1	acquisition					1687:1697	nutrient acquisition	1678:1697	nutrient acquisition	1678:1697	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	46	theme	system	1725:1730	arg1	resistance					1737:1746	resistance	1737:1746	resistance to infections	1737:1760	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	46	theme	system	1725:1730	arg1	maturation					1700:1709	maturation	1700:1709	maturation of the immune system	1700:1730	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	0	47	theme	Bacteroides	51:61	arg1	Formation					88:96	Bacteroides thetaiotaomicron Biofilm Formation	51:96	Bacteroides thetaiotaomicron Biofilm Formation	51:96	Capsular Polysaccharide Cross-Regulation Modulates Bacteroides thetaiotaomicron Biofilm Formation.					
32576670	2	48	theme	intestinal	497:506	arg1	bacterium					508:516	this intestinal bacterium	492:516	this intestinal bacterium	492:516	In contrast, adhesion and biofilm formation, which are potentially involved in gut colonization and microbiota structure and stability, have hardly been investigated in this intestinal bacterium.					
32576670	7	49	theme	bacterial	1593:1601	arg1	community					1603:1611	a complex bacterial community	1583:1611	a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections	1583:1760	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	50	theme	capsule	1336:1342	arg1	regulation					1344:1353	B. thetaiotaomicron capsule regulation	1316:1353	B. thetaiotaomicron capsule regulation	1316:1353	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	50	theme	capsule	1336:1342	arg1	determinant					1366:1376	a major determinant	1358:1376	a major determinant of B. thetaiotaomicron biofilm formation	1358:1417	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	0	51	theme	Biofilm	80:86	arg1	Formation					88:96	Bacteroides thetaiotaomicron Biofilm Formation	51:96	Bacteroides thetaiotaomicron Biofilm Formation	51:96	Capsular Polysaccharide Cross-Regulation Modulates Bacteroides thetaiotaomicron Biofilm Formation.					
32576670	7	52	dep	in	1523:1524	arg1	vitro					1526:1530	vitro	1526:1530	vitro	1526:1530	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	53	theme	B.	1316:1317	arg1	regulation					1344:1353	B. thetaiotaomicron capsule regulation	1316:1353	B. thetaiotaomicron capsule regulation	1316:1353	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	53	theme	B.	1316:1317	arg1	determinant					1366:1376	a major determinant	1358:1376	a major determinant of B. thetaiotaomicron biofilm formation	1358:1417	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	10	54	theme	mammalian	2124:2132	arg1	intestine					2134:2142	the normal mammalian intestine	2113:2142	the normal mammalian intestine	2113:2142	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	3	55	theme	thetaiotaomicron	718:733	arg1	capsules					735:742	the eight B. thetaiotaomicron capsules	705:742	the eight B. thetaiotaomicron capsules	705:742	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	7	56	theme	B.	1476:1477	arg1	structures					1504:1513	different B. thetaiotaomicron surface structures	1466:1513	different B. thetaiotaomicron surface structures	1466:1513	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	3	57	theme	biofilm	753:759	arg1	formation					761:769	biofilm formation	753:769	biofilm formation	753:769	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	3	58	theme	thetaiotaomicron	533:548	arg1	functions					566:574	B. thetaiotaomicron biofilm-related functions	530:574	B. thetaiotaomicron biofilm-related functions	530:574	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	1	59	theme	immune	282:287	arg1	system					289:294	the immune system	278:294	the immune system	278:294	Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny.					
32576670	7	60	theme	biofilm	1532:1538	arg1	formation.IMPORTANCE					1540:1559	in vitro biofilm formation.IMPORTANCE	1523:1559	in vitro biofilm formation.IMPORTANCE	1523:1559	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	12	61	theme	adhesion	2443:2450	arg1	properties					2452:2461	its adhesion properties	2439:2461	its adhesion properties	2439:2461	Studying how B. thetaiotaomicron regulates its adhesion properties will allow us to better understand the physiology and specific properties of this important gut symbiont within anaerobic biofilms.					
32576670	7	62	theme	in	1523:1524	arg1	formation.IMPORTANCE					1540:1559	in vitro biofilm formation.IMPORTANCE	1523:1559	in vitro biofilm formation.IMPORTANCE	1523:1559	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	4	63	theme	decreased	878:886	arg1	capsulation					888:898	decreased capsulation	878:898	decreased capsulation of the population	878:916	We then showed that the production of capsules 1, 2, 3, 5, and 6 also inhibits biofilm formation and that decreased capsulation of the population correlated with increased biofilm formation, suggesting that capsules could be masking adhesive surface structures.					
32576670	7	64	theme	surface	1496:1502	arg1	structures					1504:1513	different B. thetaiotaomicron surface structures	1466:1513	different B. thetaiotaomicron surface structures	1466:1513	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	11	65	theme	formation	2348:2356	arg1	capsules					2184:2191	B. thetaiotaomicron capsules	2164:2191	B. thetaiotaomicron capsules	2164:2191	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	65	theme	formation	2348:2356	arg1	determinants					2324:2335	major determinants	2318:2335	major determinants of biofilm formation that mask or unmask adhesion factors	2318:2393	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	66	theme	biofilm	2340:2346	arg1	formation					2348:2356	biofilm formation	2340:2356	biofilm formation	2340:2356	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	1	67	theme	host	200:203	arg1	health					205:210	host health	200:210	host health	200:210	Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny.					
32576670	7	68	theme	structures	1504:1513	arg1	modulation					1452:1461	modulation	1452:1461	modulation of different B. thetaiotaomicron surface structures	1452:1513	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	6	69	theme	B.	1174:1175	arg1	locus					1208:1212	the B. thetaiotaomicron glycosylation locus	1170:1212	the B. thetaiotaomicron glycosylation locus	1170:1212	Finally, we demonstrated that BT2934, the wzx homolog of the B. thetaiotaomicron glycosylation locus, competes with capsule production and impacts its adhesion capacity.					
32576670	9	70	theme	most	1975:1978	arg1	bacteria					1984:1991	most gut bacteria	1975:1991	most gut bacteria	1975:1991	However, little is known about the adhesion capacity of most gut bacteria.					
32576670	11	71	theme	gut	2292:2294	arg1	environment					2296:2306	the gut environment	2288:2306	the gut environment	2288:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	4	72	theme	biofilm	851:857	arg1	formation					859:867	biofilm formation	851:867	biofilm formation	851:867	We then showed that the production of capsules 1, 2, 3, 5, and 6 also inhibits biofilm formation and that decreased capsulation of the population correlated with increased biofilm formation, suggesting that capsules could be masking adhesive surface structures.					
32576670	9	73	theme	bacteria	1984:1991	arg1	capacity					1963:1970	the adhesion capacity	1950:1970	the adhesion capacity of most gut bacteria	1950:1991	However, little is known about the adhesion capacity of most gut bacteria.					
32576670	6	74	theme	wzx	1155:1157	arg1	BT2934					1143:1148	BT2934	1143:1148	BT2934	1143:1148	Finally, we demonstrated that BT2934, the wzx homolog of the B. thetaiotaomicron glycosylation locus, competes with capsule production and impacts its adhesion capacity.					
32576670	6	74	theme	wzx	1155:1157	arg1	homolog					1159:1165	the wzx homolog	1151:1165	the wzx homolog of the B. thetaiotaomicron glycosylation locus	1151:1212	Finally, we demonstrated that BT2934, the wzx homolog of the B. thetaiotaomicron glycosylation locus, competes with capsule production and impacts its adhesion capacity.					
32576670	12	75	theme	anaerobic	2575:2583	arg1	biofilms					2585:2592	anaerobic biofilms	2575:2592	anaerobic biofilms	2575:2592	Studying how B. thetaiotaomicron regulates its adhesion properties will allow us to better understand the physiology and specific properties of this important gut symbiont within anaerobic biofilms.					
32576670	12	76	theme	symbiont	2559:2566	arg1	physiology					2502:2511	physiology	2502:2511	physiology	2502:2511	Studying how B. thetaiotaomicron regulates its adhesion properties will allow us to better understand the physiology and specific properties of this important gut symbiont within anaerobic biofilms.					
32576670	12	76	theme	symbiont	2559:2566	arg1	properties					2526:2535	specific properties	2517:2535	specific properties	2517:2535	Studying how B. thetaiotaomicron regulates its adhesion properties will allow us to better understand the physiology and specific properties of this important gut symbiont within anaerobic biofilms.					
32576670	5	77	theme	adhesive	1092:1099	arg1	properties					1101:1110	intrinsic adhesive properties	1082:1110	intrinsic adhesive properties	1082:1110	In contrast, we showed that capsule 8 displayed intrinsic adhesive properties.					
32576670	4	78	theme	biofilm	944:950	arg1	formation					952:960	increased biofilm formation	934:960	increased biofilm formation	934:960	We then showed that the production of capsules 1, 2, 3, 5, and 6 also inhibits biofilm formation and that decreased capsulation of the population correlated with increased biofilm formation, suggesting that capsules could be masking adhesive surface structures.					
32576670	3	79	theme	biofilm-forming	629:643	arg1	strain					655:660	the poorly biofilm-forming reference strain VPI-5482	618:669	the poorly biofilm-forming reference strain VPI-5482	618:669	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	8	80	theme	called	1819:1824	arg1	biofilms					1826:1833	communities called biofilms	1807:1833	communities called biofilms	1807:1833	The capacity to adhere to surfaces and form communities called biofilms is believed to be important for niche colonization and maintenance of gut bacteria.					
32576670	1	81	theme	abundant	147:154	arg1	species					169:175	the most abundant gut symbiont species	138:175	the most abundant gut symbiont species	138:175	Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny.					
32576670	10	82	theme	biofilm	2025:2031	arg1	formation					2033:2041	biofilm formation	2025:2041	biofilm formation	2025:2041	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	1	83	theme	symbiont	160:167	arg1	species					169:175	the most abundant gut symbiont species	138:175	the most abundant gut symbiont species	138:175	Bacteroides thetaiotaomicron is one of the most abundant gut symbiont species, whose contribution to host health through its ability to degrade dietary polysaccharides and mature the immune system is under intense scrutiny.					
32576670	4	84	theme	population	907:916	arg1	capsulation					888:898	decreased capsulation	878:898	decreased capsulation of the population	878:916	We then showed that the production of capsules 1, 2, 3, 5, and 6 also inhibits biofilm formation and that decreased capsulation of the population correlated with increased biofilm formation, suggesting that capsules could be masking adhesive surface structures.					
32576670	7	85	theme	formation	1409:1417	arg1	regulation					1344:1353	B. thetaiotaomicron capsule regulation	1316:1353	B. thetaiotaomicron capsule regulation	1316:1353	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	85	theme	formation	1409:1417	arg1	determinant					1366:1376	a major determinant	1358:1376	a major determinant of B. thetaiotaomicron biofilm formation	1358:1417	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	12	86	theme	gut	2555:2557	arg1	symbiont					2559:2566	this important gut symbiont	2540:2566	this important gut symbiont	2540:2566	Studying how B. thetaiotaomicron regulates its adhesion properties will allow us to better understand the physiology and specific properties of this important gut symbiont within anaerobic biofilms.					
32576670	7	87	theme	thetaiotaomicron	1384:1399	arg1	formation					1409:1417	B. thetaiotaomicron biofilm formation	1381:1417	B. thetaiotaomicron biofilm formation	1381:1417	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	4	88	theme	masking	997:1003	arg1	structures					1022:1031	masking adhesive surface structures	997:1031	masking adhesive surface structures	997:1031	We then showed that the production of capsules 1, 2, 3, 5, and 6 also inhibits biofilm formation and that decreased capsulation of the population correlated with increased biofilm formation, suggesting that capsules could be masking adhesive surface structures.					
32576670	10	89	theme	abundant	2092:2099	arg1	bacteria					2101:2108	the most abundant bacteria	2083:2108	the most abundant bacteria of the normal mammalian intestine	2083:2142	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	8	90	theme	niche	1867:1871	arg1	colonization					1873:1884	niche colonization	1867:1884	niche colonization	1867:1884	The capacity to adhere to surfaces and form communities called biofilms is believed to be important for niche colonization and maintenance of gut bacteria.					
32576670	7	91	theme	important	1624:1632	arg1	roles					1634:1638	important roles	1624:1638	important roles	1624:1638	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	0	92	theme	Polysaccharide	9:22	arg1	Cross-Regulation					24:39	Capsular Polysaccharide Cross-Regulation	0:39	Capsular Polysaccharide Cross-Regulation	0:39	Capsular Polysaccharide Cross-Regulation Modulates Bacteroides thetaiotaomicron Biofilm Formation.					
32576670	11	93	theme	layers	2243:2248	arg1	layers					2243:2248	eight surface-exposed polysaccharidic layers	2205:2248	eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment	2205:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	93	theme	layers	2243:2248	arg1	group					2196:2200	a group	2194:2200	a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment	2194:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	11	93	theme	layers	2243:2248	arg1	capsules					2184:2191	B. thetaiotaomicron capsules	2164:2191	B. thetaiotaomicron capsules	2164:2191	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	2	94	theme	gut	402:404	arg1	colonization					406:417	gut colonization	402:417	gut colonization	402:417	In contrast, adhesion and biofilm formation, which are potentially involved in gut colonization and microbiota structure and stability, have hardly been investigated in this intestinal bacterium.					
32576670	8	95	theme	bacteria	1909:1916	arg1	maintenance					1890:1900	maintenance	1890:1900	maintenance	1890:1900	The capacity to adhere to surfaces and form communities called biofilms is believed to be important for niche colonization and maintenance of gut bacteria.					
32576670	8	95	theme	bacteria	1909:1916	arg1	colonization					1873:1884	niche colonization	1867:1884	niche colonization	1867:1884	The capacity to adhere to surfaces and form communities called biofilms is believed to be important for niche colonization and maintenance of gut bacteria.					
32576670	11	96	theme	surface-exposed	2211:2225	arg1	layers					2243:2248	eight surface-exposed polysaccharidic layers	2205:2248	eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment	2205:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	12	97	theme	important	2545:2553	arg1	symbiont					2559:2566	this important gut symbiont	2540:2566	this important gut symbiont	2540:2566	Studying how B. thetaiotaomicron regulates its adhesion properties will allow us to better understand the physiology and specific properties of this important gut symbiont within anaerobic biofilms.					
32576670	0	98	theme	thetaiotaomicron	63:78	arg1	Formation					88:96	Bacteroides thetaiotaomicron Biofilm Formation	51:96	Bacteroides thetaiotaomicron Biofilm Formation	51:96	Capsular Polysaccharide Cross-Regulation Modulates Bacteroides thetaiotaomicron Biofilm Formation.					
32576670	7	99	theme	thetaiotaomicron	1319:1334	arg1	regulation					1344:1353	B. thetaiotaomicron capsule regulation	1316:1353	B. thetaiotaomicron capsule regulation	1316:1353	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	7	99	theme	thetaiotaomicron	1319:1334	arg1	determinant					1366:1376	a major determinant	1358:1376	a major determinant of B. thetaiotaomicron biofilm formation	1358:1417	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	4	100	theme	surface	1014:1020	arg1	structures					1022:1031	masking adhesive surface structures	997:1031	masking adhesive surface structures	997:1031	We then showed that the production of capsules 1, 2, 3, 5, and 6 also inhibits biofilm formation and that decreased capsulation of the population correlated with increased biofilm formation, suggesting that capsules could be masking adhesive surface structures.					
32576670	11	101	theme	important	2260:2268	arg1	interactions					2270:2281	important interactions	2260:2281	important interactions with the gut environment	2260:2306	We identified that B. thetaiotaomicron capsules, a group of eight surface-exposed polysaccharidic layers mediating important interactions with the gut environment, are also major determinants of biofilm formation that mask or unmask adhesion factors.					
32576670	12	102	theme	specific	2517:2524	arg1	properties					2526:2535	specific properties	2517:2535	specific properties	2517:2535	Studying how B. thetaiotaomicron regulates its adhesion properties will allow us to better understand the physiology and specific properties of this important gut symbiont within anaerobic biofilms.					
32576670	3	103	theme	B.	715:716	arg1	capsules					735:742	the eight B. thetaiotaomicron capsules	705:742	the eight B. thetaiotaomicron capsules	705:742	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	3	104	theme	B.	530:531	arg1	functions					566:574	B. thetaiotaomicron biofilm-related functions	530:574	B. thetaiotaomicron biofilm-related functions	530:574	To uncover B. thetaiotaomicron biofilm-related functions, we performed a transposon mutagenesis in the poorly biofilm-forming reference strain VPI-5482 and showed that capsule 4, one of the eight B. thetaiotaomicron capsules, hinders biofilm formation.					
32576670	7	105	theme	complex	1585:1591	arg1	community					1603:1611	a complex bacterial community	1583:1611	a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections	1583:1760	This study therefore establishes B. thetaiotaomicron capsule regulation as a major determinant of B. thetaiotaomicron biofilm formation, providing new insights into how modulation of different B. thetaiotaomicron surface structures affects in vitro biofilm formation.IMPORTANCE The human gut harbors a complex bacterial community that plays important roles in host health and disease, including nutrient acquisition, maturation of the immune system, and resistance to infections.					
32576670	10	106	theme	normal	2117:2122	arg1	intestine					2134:2142	the normal mammalian intestine	2113:2142	the normal mammalian intestine	2113:2142	In this study, we investigated biofilm formation in Bacteroides thetaiotaomicron, one of the most abundant bacteria of the normal mammalian intestine.					
32576670	2	107	theme	biofilm	349:355	arg1	formation					357:365	biofilm formation	349:365	biofilm formation	349:365	In contrast, adhesion and biofilm formation, which are potentially involved in gut colonization and microbiota structure and stability, have hardly been investigated in this intestinal bacterium.					
32232559	0	0	theme	non-organogenic	77:91	arg1	calli					93:97	the organogenic and non-organogenic calli	57:97	the organogenic and non-organogenic calli of Actinidia arguta	57:117	Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta.					
32232559	2	1	theme	morphogenic	492:502	arg1	competence					504:513	the morphogenic competence	488:513	the morphogenic competence of a tissue	488:525	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	9	2	dep	endosperm	1778:1786	arg1	LM25					1798:1801	LM25	1798:1801	LM25	1798:1801	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	9	2	dep	endosperm	1778:1786	arg1	LM21					1789:1792	LM21	1789:1792	LM21	1789:1792	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	9	3	theme	LM21	1909:1912	arg1	loss					1901:1904	loss	1901:1904	loss of LM21 and LM25	1901:1921	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	1	4	theme	callus	260:265	arg1	tissue					267:272	the callus tissue	256:272	the callus tissue that originated from the isolated endosperm of kiwifruit	256:329	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	2	5	from	layer	446:450	arg1	surface					459:465	its surface	455:465	its surface	455:465	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	9	6	dep	LM21	1789:1792	arg1	xyloglucan					1821:1830	xyloglucan	1821:1830	xyloglucan	1821:1830	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	9	6	dep	LM21	1789:1792	arg1	heteromannan					1804:1815	heteromannan	1804:1815	heteromannan	1804:1815	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	9	7	dep	loss	1901:1904	arg1	increase					1938:1945	increase	1938:1945	increase in the content of LM5	1938:1967	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	9	7	dep	loss	1901:1904	arg1	appearance					1924:1933	appearance	1924:1933	appearance	1924:1933	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	9	7	dep	loss	1901:1904	arg1	epitopes					2017:2024	LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes	1970:2024	LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes	1970:2024	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	1	8	theme	structural	159:168	arg1	organisation					170:181	the structural organisation	155:181	the structural organisation	155:181	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	8	9	from	tissues	1393:1399	arg1	matrices					1349:1356	The extracellular matrices	1331:1356	The extracellular matrices from both the non- and organogenic tissues	1331:1399	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	9	from	tissues	1393:1399	arg1	abundant					1406:1413	abundant	1406:1413	abundant	1406:1413	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	1	10	theme	tissue	267:272	arg1	competence					242:251	the morphogenic competence	226:251	the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit	226:329	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	9	11	theme	LM5	1965:1967	arg1	content					1954:1960	the content	1950:1960	the content of LM5	1950:1967	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	5	12	theme	bright	965:970	arg1	microscopy					1014:1023	bright field, fluorescence and scanning electron microscopy	965:1023	microscopy	1014:1023	The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli.					
32232559	9	13	theme	endosperm-derived	1845:1861	arg1	cells					1863:1867	the endosperm-derived cells	1841:1867	the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes)	1841:2025	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	8	14	theme	arabinogalactan	1613:1627	arg1	LM2					1638:1640	LM2	1638:1640	LM2	1638:1640	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	14	theme	arabinogalactan	1613:1627	arg1	protein					1629:1635	the arabinogalactan protein	1609:1635	the arabinogalactan protein (LM2)	1609:1641	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	4	15	theme	callus	731:736	arg1	samples					738:744	callus samples	731:744	callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway	731:922	The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway.					
32232559	7	16	theme	attached	1268:1275	arg1	cells					1277:1281	loosely attached cells	1260:1281	loosely attached cells	1260:1281	The non-organogenic tissue was composed of loosely attached cells, which were connected via a net-like structure.					
32232559	5	17	theme	field	972:976	arg1	microscopy					1014:1023	bright field, fluorescence and scanning electron microscopy	965:1023	microscopy	1014:1023	The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli.					
32232559	3	18	located	found	574:578	arg1	study					545:549	the presented study	531:549	the presented study	531:549	In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta.					
32232559	3	18	located	found	574:578	arg2	relationship					557:568	this relationship	552:568	this relationship	552:568	In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta.					
32232559	3	18	located	found	574:578	arg1	tissue					594:599	the callus tissue	583:599	the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta	583:691	In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta.					
32232559	2	19	theme	tissue	520:525	arg1	competence					504:513	the morphogenic competence	488:513	the morphogenic competence of a tissue	488:525	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	0	20	from	diverse	46:52	arg1	calli					93:97	the organogenic and non-organogenic calli	57:97	the organogenic and non-organogenic calli of Actinidia arguta	57:117	Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta.					
32232559	9	21	from	appearance	1924:1933	arg1	content					1954:1960	the content	1950:1960	the content of LM5	1950:1967	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	4	22	theme	age	766:768	arg1	samples					738:744	callus samples	731:744	callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway	731:922	The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway.					
32232559	8	23	from	homogalacturonan	1425:1440	arg1	matrices					1349:1356	The extracellular matrices	1331:1356	The extracellular matrices from both the non- and organogenic tissues	1331:1399	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	23	from	homogalacturonan	1425:1440	arg1	abundant					1406:1413	abundant	1406:1413	abundant	1406:1413	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	0	24	theme	Actinidia	102:110	arg1	arguta					112:117	Actinidia arguta	102:117	Actinidia arguta	102:117	Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta.					
32232559	3	25	theme	presented	535:543	arg1	study					545:549	the presented study	531:549	the presented study	531:549	In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta.					
32232559	8	26	theme	pectic	1418:1423	arg1	homogalacturonan					1425:1440	pectic homogalacturonan	1418:1440	pectic homogalacturonan	1418:1440	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	0	27	theme	arguta	112:117	arg1	calli					93:97	the organogenic and non-organogenic calli	57:97	the organogenic and non-organogenic calli of Actinidia arguta	57:117	Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta.					
32232559	6	28	theme	cells	1172:1176	arg1	walls					1148:1152	the outer walls	1138:1152	the outer walls of the peripheral cells	1138:1176	The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures.					
32232559	4	29	theme	controlled	841:850	arg1	conditions					852:861	controlled conditions	841:861	controlled conditions promoting either an organogenic or a non-organogenic pathway	841:922	The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway.					
32232559	2	30	dep	composition	345:355	arg1	The					332:334	The	332:334	The	332:334	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	0	31	theme	Extracellular	0:12	arg1	matrix					14:19	Extracellular matrix	0:19	Extracellular matrix	0:19	Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta.					
32232559	6	32	theme	peripheral	1161:1170	arg1	cells					1172:1176	the peripheral cells	1157:1176	the peripheral cells	1157:1176	The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures.					
32232559	2	33	theme	matrix	406:411	arg1	composition					345:355	chemical composition	336:355	chemical composition	336:355	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	2	33	theme	matrix	406:411	arg1	organisation					372:383	structural organisation	361:383	structural organisation	361:383	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	0	34	theme	wall	25:28	arg1	composition					30:40	wall composition	25:40	wall composition	25:40	Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta.					
32232559	1	35	from	Differences	120:130	arg1	organisation					170:181	the structural organisation	155:181	the structural organisation	155:181	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	1	35	from	Differences	120:130	arg1	composition					139:149	composition	139:149	composition	139:149	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	1	36	dep	composition	139:149	arg1	the					135:137	the	135:137	the	135:137	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	4	37	theme	isolated	798:805	arg1	endosperm					807:815	an isolated endosperm	795:815	an isolated endosperm	795:815	The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway.					
32232559	1	38	theme	matrix	204:209	arg1	organisation					170:181	the structural organisation	155:181	the structural organisation	155:181	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	1	38	theme	matrix	204:209	arg1	composition					139:149	composition	139:149	composition	139:149	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	8	39	theme	organogenic	1381:1391	arg1	tissues					1393:1399	organogenic tissues	1381:1399	organogenic tissues	1381:1399	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	1	40	theme	isolated	299:306	arg1	endosperm					308:316	the isolated endosperm	295:316	the isolated endosperm of kiwifruit	295:329	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	3	41	theme	isolated	639:646	arg1	endosperm					648:656	the isolated endosperm	635:656	the isolated endosperm of the kiwiberry	635:673	In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta.					
32232559	6	42	theme	organogenic	1103:1113	arg1	compact					1126:1132	compact	1126:1132	compact	1126:1132	The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures.					
32232559	6	42	theme	organogenic	1103:1113	arg1	tissue					1115:1120	The organogenic tissue	1099:1120	The organogenic tissue	1099:1120	The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures.					
32232559	8	43	theme	extracellular	1335:1347	arg1	matrices					1349:1356	The extracellular matrices	1331:1356	The extracellular matrices from both the non- and organogenic tissues	1331:1399	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	43	theme	extracellular	1335:1347	arg1	abundant					1406:1413	abundant	1406:1413	abundant	1406:1413	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	7	44	theme	net-like	1311:1318	arg1	structure					1320:1328	a net-like structure	1309:1328	a net-like structure	1309:1328	The non-organogenic tissue was composed of loosely attached cells, which were connected via a net-like structure.					
32232559	6	45	theme	outer	1142:1146	arg1	walls					1148:1152	the outer walls	1138:1152	the outer walls of the peripheral cells	1138:1176	The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures.					
32232559	5	46	theme	calli	1092:1096	arg1	types					1083:1087	the two types	1075:1087	the two types of calli	1075:1096	The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli.					
32232559	3	47	theme	callus	587:592	arg1	tissue					594:599	the callus tissue	583:599	the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta	583:691	In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta.					
32232559	1	48	theme	extracellular	190:202	arg1	matrix					204:209	the extracellular matrix	186:209	the extracellular matrix	186:209	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	9	49	from	increase	1938:1945	arg1	content					1954:1960	the content	1950:1960	the content of LM5	1950:1967	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	9	50	dep	undergo	1874:1880	arg1	loss					1901:1904	loss	1901:1904	loss of LM21 and LM25	1901:1921	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	2	51	theme	cell	428:431	arg1	wall					433:436	the cell wall	424:436	the cell wall	424:436	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	1	52	theme	kiwifruit	321:329	arg1	endosperm					308:316	the isolated endosperm	295:316	the isolated endosperm of kiwifruit	295:329	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	5	53	theme	significant	1043:1053	arg1	differences					1055:1065	significant differences	1043:1065	significant differences between the two types of calli	1043:1096	The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli.					
32232559	9	54	theme	LM25	1918:1921	arg1	loss					1901:1904	loss	1901:1904	loss of LM21 and LM25	1901:1921	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	2	55	theme	structural	361:370	arg1	organisation					372:383	structural organisation	361:383	structural organisation	361:383	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	8	56	theme	non-organogenic	1669:1683	arg1	callus					1685:1690	the non-organogenic callus	1665:1690	the non-organogenic callus	1665:1690	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	57	from	extensins	1446:1454	arg1	matrices					1349:1356	The extracellular matrices	1331:1356	The extracellular matrices from both the non- and organogenic tissues	1331:1399	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	57	from	extensins	1446:1454	arg1	abundant					1406:1413	abundant	1406:1413	abundant	1406:1413	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	3	58	theme	kiwiberry	665:673	arg1	endosperm					648:656	the isolated endosperm	635:656	the isolated endosperm of the kiwiberry	635:673	In the presented study, this relationship was found in the callus tissue that had been differentiated from the isolated endosperm of the kiwiberry, Actinidia arguta.					
32232559	4	59	theme	experimental	698:709	arg1	system					711:716	The experimental system	694:716	The experimental system	694:716	The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway.					
32232559	9	60	link	endosperm-derived	1845:1861	arg1	cells					1863:1867	the endosperm-derived cells	1841:1867	the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes)	1841:2025	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	4	61	theme	non-organogenic	900:914	arg1	pathway					916:922	a non-organogenic pathway	898:922	a non-organogenic pathway	898:922	The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway.					
32232559	8	62	located	detected	1648:1655	arg1	callus					1685:1690	the non-organogenic callus	1665:1690	the non-organogenic callus	1665:1690	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	62	located	detected	1648:1655	arg2	characteristic					1529:1542	characteristic	1529:1542	characteristic	1529:1542	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	62	located	detected	1648:1655	arg2	epitopes					1511:1518	the epitopes	1507:1518	the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2)	1507:1641	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	63	from	non-	1372:1375	arg1	matrices					1349:1356	The extracellular matrices	1331:1356	The extracellular matrices from both the non- and organogenic tissues	1331:1399	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	63	from	non-	1372:1375	arg1	abundant					1406:1413	abundant	1406:1413	abundant	1406:1413	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	1	64	theme	morphogenic	230:240	arg1	competence					242:251	the morphogenic competence	226:251	the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit	226:329	Differences in the composition and the structural organisation of the extracellular matrix correlate with the morphogenic competence of the callus tissue that originated from the isolated endosperm of kiwifruit.					
32232559	4	65	theme	same	761:764	arg1	age					766:768	exactly the same age	749:768	exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway	749:922	The experimental system was based on callus samples of exactly the same age that had originated from an isolated endosperm but were cultured under controlled conditions promoting either an organogenic or a non-organogenic pathway.					
32232559	5	66	theme	scanning	996:1003	arg1	microscopy					1014:1023	bright field, fluorescence and scanning electron microscopy	965:1023	microscopy	1014:1023	The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli.					
32232559	2	67	from	wall	433:436	arg1	surface					459:465	its surface	455:465	its surface	455:465	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	2	68	theme	chemical	336:343	arg1	composition					345:355	chemical composition	336:355	chemical composition	336:355	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	0	69	theme	organogenic	61:71	arg1	calli					93:97	the organogenic and non-organogenic calli	57:97	the organogenic and non-organogenic calli of Actinidia arguta	57:117	Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta.					
32232559	0	70	from	calli	93:97	arg1	diverse					46:52	diverse	46:52	diverse	46:52	Extracellular matrix and wall composition are diverse in the organogenic and non-organogenic calli of Actinidia arguta.					
32232559	5	71	theme	electron	1005:1012	arg1	microscopy					1014:1023	bright field, fluorescence and scanning electron microscopy	965:1023	microscopy	1014:1023	The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli.					
32232559	2	72	theme	extracellular	392:404	arg1	matrix					406:411	the extracellular matrix	388:411	the extracellular matrix	388:411	The chemical composition and structural organisation of the extracellular matrix, including the cell wall and the layer on its surface, may correspond with the morphogenic competence of a tissue.					
32232559	9	73	theme	Actinidia	1768:1776	arg1	endosperm					1778:1786	the Actinidia endosperm	1764:1786	the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan)	1764:1831	Moreover, we report the epitopes, which presence is characteristic for the Actinidia endosperm (LM21 and LM25, heteromannan and xyloglucan) and for the endosperm-derived cells that undergo dedifferentiation (loss of LM21 and LM25; appearance or increase in the content of LM5, LM6, LM19, JIM11, JIM12, JIM20, JIM8 and JIM16 epitopes).					
32232559	5	74	theme	microscopy	1014:1023	arg1	techniques					1025:1034	bright field, fluorescence and scanning electron microscopy techniques	965:1034	bright field, fluorescence and scanning electron microscopy techniques	965:1034	The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli.					
32232559	6	75	theme	granular	1196:1203	arg1	structures					1205:1214	granular structures	1196:1214	granular structures	1196:1214	The organogenic tissue was compact and the outer walls of the peripheral cells were covered with granular structures.					
32232559	5	76	theme	fluorescence	979:990	arg1	microscopy					1014:1023	bright field, fluorescence and scanning electron microscopy	965:1023	microscopy	1014:1023	The analyses which were performed using bright field, fluorescence and scanning electron microscopy techniques showed significant differences between the two types of calli.					
32232559	8	77	from	abundant	1406:1413	arg1	homogalacturonan					1425:1440	pectic homogalacturonan	1418:1440	pectic homogalacturonan	1418:1440	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	77	from	abundant	1406:1413	arg1	extensins					1446:1454	extensins	1446:1454	extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes)	1446:1500	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	8	77	from	abundant	1406:1413	arg1	epitopes					1492:1499	LM19, LM20, JIM11, JIM12 and JIM20 epitopes	1457:1499	LM19, LM20, JIM11, JIM12 and JIM20 epitopes	1457:1499	The extracellular matrices from both the non- and organogenic tissues were abundant in pectic homogalacturonan and extensins (LM19, LM20, JIM11, JIM12 and JIM20 epitopes), but the epitopes that are characteristic for rhamnogalacturonan I (LM5 and LM6), hemicellulose (LM25) and the arabinogalactan protein (LM2) were detected only in the non-organogenic callus.					
32232559	7	78	theme	non-organogenic	1221:1235	arg1	tissue					1237:1242	The non-organogenic tissue	1217:1242	The non-organogenic tissue	1217:1242	The non-organogenic tissue was composed of loosely attached cells, which were connected via a net-like structure.					
33941040	7	0	theme	materials	1112:1120	arg1	generation					1085:1094	the next generation	1076:1094	the next generation of pulp capping materials	1076:1120	These composites demonstrate a promising potential as the next generation of pulp capping materials.					
33941040	1	1	theme	decaying	251:258	arg1	teeth					260:264	decaying teeth	251:264	decaying teeth	251:264	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	5	2	theme	particle	763:770	arg1	size					772:775	The particle size	759:775	The particle size	759:775	The particle size, surface, and mechanical properties of these materials were characterized.					
33941040	3	3	dep	proliferation	545:557	arg1	the					541:543	the	541:543	the	541:543	To achieve this goal a material needs to crosslink quickly, be structurally rigid, and support the proliferation and differentiation of stem cells contained within the dental pulp.					
33941040	1	4	theme	teeth	260:264	arg1	function					239:246	function	239:246	function	239:246	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	1	4	theme	teeth	260:264	arg1	form					230:233	form	230:233	form	230:233	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	7	5	theme	pulp	1099:1102	arg1	materials					1112:1120	pulp capping materials	1099:1120	pulp capping materials	1099:1120	These composites demonstrate a promising potential as the next generation of pulp capping materials.					
33941040	5	6	theme	mechanical	791:800	arg1	properties					802:811	mechanical properties	791:811	mechanical properties	791:811	The particle size, surface, and mechanical properties of these materials were characterized.					
33941040	7	7	theme	capping	1104:1110	arg1	materials					1112:1120	pulp capping materials	1099:1120	pulp capping materials	1099:1120	These composites demonstrate a promising potential as the next generation of pulp capping materials.					
33941040	4	8	theme	ether	752:756	arg1	hydrogels					699:707	polymer hydrogels	691:707	polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether	691:756	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
33941040	2	9	theme	capping	322:328	arg1	agent					330:334	a possible regenerative pulp capping agent	293:334	a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time	293:443	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	4	10	theme	polymer	691:697	arg1	hydrogels					699:707	polymer hydrogels	691:707	polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether	691:756	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
33941040	1	11	dep	prevalent	123:131	arg1	preventable					134:144	preventable	134:144	preventable	134:144	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	4	12	theme	calcium	642:648	arg1	nanoparticles					660:672	calcium phosphate nanoparticles	642:672	calcium phosphate nanoparticles	642:672	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
33941040	2	13	theme	healthy	396:402	arg1	tissue					411:416	healthy dentin tissue	396:416	healthy dentin tissue	396:416	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	1	14	theme	Dental	94:99	arg1	worldwide					154:162	the most prevalent, preventable disease worldwide	114:162	the most prevalent, preventable disease worldwide	114:162	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	1	14	theme	Dental	94:99	arg1	cavities					101:108	Dental cavities	94:108	Dental cavities	94:108	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	4	15	from	study	635:639	arg1	embedded					679:686	embedded	679:686	embedded	679:686	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
33941040	1	16	theme	robust	185:190	arg1	options					202:208	robust treatment options	185:208	robust treatment options	185:208	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	0	17	theme	chitosan-calcium	8:23	arg1	composites					35:44	Tunable chitosan-calcium phosphate composites	0:44	Tunable chitosan-calcium phosphate composites as cell-instructive dental pulp capping agents.	0:92	Tunable chitosan-calcium phosphate composites as cell-instructive dental pulp capping agents.					
33941040	2	18	theme	pulp	317:320	arg1	agent					330:334	a possible regenerative pulp capping agent	293:334	a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time	293:443	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	1	19	theme	treatment	192:200	arg1	options					202:208	robust treatment options	185:208	robust treatment options	185:208	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	0	20	theme	Tunable	0:6	arg1	composites					35:44	Tunable chitosan-calcium phosphate composites	0:44	Tunable chitosan-calcium phosphate composites as cell-instructive dental pulp capping agents.	0:92	Tunable chitosan-calcium phosphate composites as cell-instructive dental pulp capping agents.					
33941040	7	21	theme	next	1080:1083	arg1	generation					1085:1094	the next generation	1076:1094	the next generation of pulp capping materials	1076:1120	These composites demonstrate a promising potential as the next generation of pulp capping materials.					
33941040	7	22	theme	promising	1053:1061	arg1	potential					1063:1071	a promising potential	1051:1071	a promising potential as the next generation of pulp capping materials	1051:1120	These composites demonstrate a promising potential as the next generation of pulp capping materials.					
33941040	2	23	theme	regenerative	304:315	arg1	agent					330:334	a possible regenerative pulp capping agent	293:334	a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time	293:443	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	4	24	theme	carboxymethyl-chitosan	712:733	arg1	hydrogels					699:707	polymer hydrogels	691:707	polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether	691:756	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
33941040	6	25	contain	have	869:872	arg2	moduli					874:879	moduli	874:879	moduli up to 3 MPa	874:891	These composites have moduli up to 3 MPa, support the culture of dental pulp stem cells more than 3 weeks and exhibit osteogenic potential even without osteogenic media.					
33941040	6	25	contain	have	869:872	arg1	composites					858:867	These composites	852:867	These composites	852:867	These composites have moduli up to 3 MPa, support the culture of dental pulp stem cells more than 3 weeks and exhibit osteogenic potential even without osteogenic media.					
33941040	2	26	theme	possible	295:302	arg1	agent					330:334	a possible regenerative pulp capping agent	293:334	a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time	293:443	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	0	27	theme	phosphate	25:33	arg1	composites					35:44	Tunable chitosan-calcium phosphate composites	0:44	Tunable chitosan-calcium phosphate composites as cell-instructive dental pulp capping agents.	0:92	Tunable chitosan-calcium phosphate composites as cell-instructive dental pulp capping agents.					
33941040	6	28	theme	more	940:943	arg1	cells					934:938	dental pulp stem cells	917:938	dental pulp stem cells more than 3 weeks	917:956	These composites have moduli up to 3 MPa, support the culture of dental pulp stem cells more than 3 weeks and exhibit osteogenic potential even without osteogenic media.					
33941040	4	29	theme	a	739:739	arg1	ether					752:756	a diglycidyl ether	739:756	a diglycidyl ether	739:756	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
33941040	5	30	theme	materials	822:830	arg1	surface					778:784	surface	778:784	surface	778:784	The particle size, surface, and mechanical properties of these materials were characterized.					
33941040	5	30	theme	materials	822:830	arg1	size					772:775	The particle size	759:775	The particle size	759:775	The particle size, surface, and mechanical properties of these materials were characterized.					
33941040	5	30	theme	materials	822:830	arg1	properties					802:811	mechanical properties	791:811	mechanical properties	791:811	The particle size, surface, and mechanical properties of these materials were characterized.					
33941040	0	31	theme	cell-instructive	49:64	arg1	agents					86:91	cell-instructive dental pulp capping agents	49:91	cell-instructive dental pulp capping agents	49:91	Tunable chitosan-calcium phosphate composites as cell-instructive dental pulp capping agents.					
33941040	6	32	theme	cells	934:938	arg1	culture					906:912	the culture	902:912	the culture of dental pulp stem cells more than 3 weeks	902:956	These composites have moduli up to 3 MPa, support the culture of dental pulp stem cells more than 3 weeks and exhibit osteogenic potential even without osteogenic media.					
33941040	6	33	theme	stem	929:932	arg1	cells					934:938	dental pulp stem cells	917:938	dental pulp stem cells more than 3 weeks	917:956	These composites have moduli up to 3 MPa, support the culture of dental pulp stem cells more than 3 weeks and exhibit osteogenic potential even without osteogenic media.					
33941040	6	34	theme	osteogenic	970:979	arg1	potential					981:989	osteogenic potential	970:989	osteogenic potential	970:989	These composites have moduli up to 3 MPa, support the culture of dental pulp stem cells more than 3 weeks and exhibit osteogenic potential even without osteogenic media.					
33941040	1	35	dep	form	230:233	arg1	the					226:228	the	226:228	the	226:228	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	3	36	theme	stem	582:585	arg1	cells					587:591	stem cells	582:591	stem cells contained within the dental pulp	582:624	To achieve this goal a material needs to crosslink quickly, be structurally rigid, and support the proliferation and differentiation of stem cells contained within the dental pulp.					
33941040	2	37	theme	dentin	404:409	arg1	tissue					411:416	healthy dentin tissue	396:416	healthy dentin tissue	396:416	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	0	38	theme	pulp	73:76	arg1	agents					86:91	cell-instructive dental pulp capping agents	49:91	cell-instructive dental pulp capping agents	49:91	Tunable chitosan-calcium phosphate composites as cell-instructive dental pulp capping agents.					
33941040	6	39	theme	pulp	924:927	arg1	cells					934:938	dental pulp stem cells	917:938	dental pulp stem cells more than 3 weeks	917:956	These composites have moduli up to 3 MPa, support the culture of dental pulp stem cells more than 3 weeks and exhibit osteogenic potential even without osteogenic media.					
33941040	1	40	theme	prevalent	123:131	arg1	cavities					101:108	Dental cavities	94:108	Dental cavities	94:108	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	1	40	theme	prevalent	123:131	arg1	worldwide					154:162	the most prevalent, preventable disease worldwide	114:162	the most prevalent, preventable disease worldwide	114:162	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	0	41	theme	dental	66:71	arg1	agents					86:91	cell-instructive dental pulp capping agents	49:91	cell-instructive dental pulp capping agents	49:91	Tunable chitosan-calcium phosphate composites as cell-instructive dental pulp capping agents.					
33941040	6	42	theme	dental	917:922	arg1	cells					934:938	dental pulp stem cells	917:938	dental pulp stem cells more than 3 weeks	917:956	These composites have moduli up to 3 MPa, support the culture of dental pulp stem cells more than 3 weeks and exhibit osteogenic potential even without osteogenic media.					
33941040	3	43	theme	dental	614:619	arg1	pulp					621:624	the dental pulp	610:624	the dental pulp	610:624	To achieve this goal a material needs to crosslink quickly, be structurally rigid, and support the proliferation and differentiation of stem cells contained within the dental pulp.					
33941040	4	44	theme	phosphate	650:658	arg1	nanoparticles					660:672	calcium phosphate nanoparticles	642:672	calcium phosphate nanoparticles	642:672	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
33941040	2	45	theme	agent	330:334	arg1	presentation					277:288	a presentation	275:288	a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time	275:443	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	2	45	theme	agent	330:334	arg1	Here					267:270	Here	267:270	Here	267:270	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	4	46	theme	diglycidyl	741:750	arg1	ether					752:756	a diglycidyl ether	739:756	a diglycidyl ether	739:756	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
33941040	3	47	dep	needs	478:482	arg1	achieve					449:455	achieve	449:455	To achieve this goal	446:465	To achieve this goal a material needs to crosslink quickly, be structurally rigid, and support the proliferation and differentiation of stem cells contained within the dental pulp.					
33941040	1	48	theme	disease	146:152	arg1	cavities					101:108	Dental cavities	94:108	Dental cavities	94:108	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	1	48	theme	disease	146:152	arg1	worldwide					154:162	the most prevalent, preventable disease worldwide	114:162	the most prevalent, preventable disease worldwide	114:162	Dental cavities are the most prevalent, preventable disease worldwide providing a need for robust treatment options to restore both the form and function of decaying teeth.					
33941040	6	49	theme	osteogenic	1004:1013	arg1	media					1015:1019	osteogenic media	1004:1019	osteogenic media	1004:1019	These composites have moduli up to 3 MPa, support the culture of dental pulp stem cells more than 3 weeks and exhibit osteogenic potential even without osteogenic media.					
33941040	3	50	theme	cells	587:591	arg1	differentiation					563:577	differentiation	563:577	differentiation	563:577	To achieve this goal a material needs to crosslink quickly, be structurally rigid, and support the proliferation and differentiation of stem cells contained within the dental pulp.					
33941040	3	50	theme	cells	587:591	arg1	proliferation					545:557	proliferation	545:557	proliferation	545:557	To achieve this goal a material needs to crosslink quickly, be structurally rigid, and support the proliferation and differentiation of stem cells contained within the dental pulp.					
33941040	2	51	theme	tooth	362:366	arg1	function					368:375	tooth function	362:375	tooth function	362:375	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	4	52	from	hydrogels	699:707	arg1	embedded					679:686	embedded	679:686	embedded	679:686	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
33941040	6	53	dep	3 MPa	887:891	arg1	up					881:882	up	881:882	up	881:882	These composites have moduli up to 3 MPa, support the culture of dental pulp stem cells more than 3 weeks and exhibit osteogenic potential even without osteogenic media.					
33941040	0	54	theme	capping	78:84	arg1	agents					86:91	cell-instructive dental pulp capping agents	49:91	cell-instructive dental pulp capping agents	49:91	Tunable chitosan-calcium phosphate composites as cell-instructive dental pulp capping agents.					
33941040	2	55	theme	long	425:428	arg1	period					430:435	a long period	423:435	a long period of time	423:443	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	2	56	theme	time	440:443	arg1	period					430:435	a long period	423:435	a long period of time	423:443	Here is a presentation of a possible regenerative pulp capping agent that can serve to restore tooth function while regenerating healthy dentin tissue over a long period of time.					
33941040	4	57	from	embedded	679:686	arg1	study					635:639	this study	630:639	this study	630:639	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
33941040	4	57	from	embedded	679:686	arg1	hydrogels					699:707	polymer hydrogels	691:707	polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether	691:756	In this study, calcium phosphate nanoparticles were embedded in polymer hydrogels of carboxymethyl-chitosan and a diglycidyl ether.					
34726315	0	0	theme	Arabidopsis	94:104	arg1	reproduction					78:89	sexual reproduction	71:89	sexual reproduction of Arabidopsis	71:104	Glucuronidation of type II arabinogalactan polysaccharides function in sexual reproduction of Arabidopsis.					
34726315	3	1	from	development	740:750	arg1	glcat14a/b					806:815	glcat14a/b	806:815	glcat14a/b	806:815	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	1	from	development	740:750	arg1	mutants					834:840	glcat14a/b/c mutants	821:840	glcat14a/b/c mutants	821:840	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	2	from	collapse	777:784	arg1	glcat14a/b					806:815	glcat14a/b	806:815	glcat14a/b	806:815	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	2	from	collapse	777:784	arg1	mutants					834:840	glcat14a/b/c mutants	821:840	glcat14a/b/c mutants	821:840	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	1	3	theme	plant	170:174	arg1	proteins					123:130	Arabinogalactan proteins	107:130	Arabinogalactan proteins (AGPs)	107:137	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	1	3	theme	plant	170:174	arg1	proteins					186:193	complex, hyperglycosylated plant cell wall proteins	143:193	complex, hyperglycosylated plant cell wall proteins	143:193	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	1	4	theme	moieties	256:263	arg1	roles					234:238	the biological roles	219:238	the biological roles of their glycan moieties in sexual reproduction	219:286	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	7	5	theme	reproductive	1522:1533	arg1	development					1535:1545	reproductive development	1522:1545	reproductive development	1522:1545	This work demonstrates the important roles that AG glucuronidation plays in Arabidopsis sexual reproduction and reproductive development.					
34726315	6	6	theme	AGP	1312:1314	arg1	epitopes					1322:1329	AGP sugar epitopes	1312:1329	AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants	1312:1379	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	1	7	theme	cell	176:179	arg1	proteins					123:130	Arabinogalactan proteins	107:130	Arabinogalactan proteins (AGPs)	107:137	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	1	7	theme	cell	176:179	arg1	proteins					186:193	complex, hyperglycosylated plant cell wall proteins	143:193	complex, hyperglycosylated plant cell wall proteins	143:193	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	4	8	theme	Synchronous	843:853	arg1	development					855:865	Synchronous development	843:865	Synchronous development between locules within the same anther	843:904	Synchronous development between locules within the same anther was also lost in some glcat14a/b/c stamens.					
34726315	7	9	theme	important	1437:1445	arg1	roles					1447:1451	the important roles	1433:1451	the important roles that AG glucuronidation plays in Arabidopsis sexual reproduction and reproductive development	1433:1545	This work demonstrates the important roles that AG glucuronidation plays in Arabidopsis sexual reproduction and reproductive development.					
34726315	1	10	theme	sexual	268:273	arg1	reproduction					275:286	sexual reproduction	268:286	sexual reproduction	268:286	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	6	11	theme	wild	1399:1402	arg1	type					1404:1407	the wild type	1395:1407	the wild type	1395:1407	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	1	12	dep	complex	143:149	arg1	hyperglycosylated					152:168	hyperglycosylated	152:168	hyperglycosylated	152:168	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	3	13	theme	pollen	789:794	arg1	grains					796:801	pollen grains	789:801	pollen grains	789:801	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	6	14	theme	decreased	1291:1299	arg1	epitopes					1322:1329	AGP sugar epitopes	1312:1329	AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants	1312:1379	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	6	14	theme	decreased	1291:1299	arg1	amounts					1301:1307	decreased amounts	1291:1307	decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants	1291:1379	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	7	15	theme	AG	1458:1459	arg1	glucuronidation					1461:1475	AG glucuronidation	1458:1475	AG glucuronidation	1458:1475	This work demonstrates the important roles that AG glucuronidation plays in Arabidopsis sexual reproduction and reproductive development.					
34726315	6	16	from	sugars	1181:1186	arg1	glcat14a/b					1191:1200	glcat14a/b	1191:1200	glcat14a/b	1191:1200	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	1	17	theme	wall	181:184	arg1	proteins					123:130	Arabinogalactan proteins	107:130	Arabinogalactan proteins (AGPs)	107:137	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	1	17	theme	wall	181:184	arg1	proteins					186:193	complex, hyperglycosylated plant cell wall proteins	143:193	complex, hyperglycosylated plant cell wall proteins	143:193	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	2	18	theme	embryo	484:489	arg1	development					491:501	normal embryo development	477:501	normal embryo development	477:501	Here, we report that GLCAT14A, GLCAT14B, and GLCAT14C, three enzymes responsible for the addition of glucuronic acid residues to AGPs, function in pollen development, polytubey block, and normal embryo development in Arabidopsis.					
34726315	3	19	theme	reticulate	691:700	arg1	structure					702:710	the reticulate structure	687:710	the reticulate structure of the exine wall	687:728	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	2	20	theme	polytubey	456:464	arg1	block					466:470	polytubey block	456:470	polytubey block	456:470	Here, we report that GLCAT14A, GLCAT14B, and GLCAT14C, three enzymes responsible for the addition of glucuronic acid residues to AGPs, function in pollen development, polytubey block, and normal embryo development in Arabidopsis.					
34726315	7	21	theme	sexual	1498:1503	arg1	reproduction					1505:1516	Arabidopsis sexual reproduction	1486:1516	Arabidopsis sexual reproduction	1486:1516	This work demonstrates the important roles that AG glucuronidation plays in Arabidopsis sexual reproduction and reproductive development.					
34726315	3	22	theme	structure	702:710	arg1	collapse					777:784	collapse	777:784	collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants	777:840	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	22	theme	structure	702:710	arg1	disorganization					668:682	disorganization	668:682	disorganization of the reticulate structure of the exine wall	668:728	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	22	theme	structure	702:710	arg1	development					740:750	abnormal development	731:750	abnormal development of the intine layer	731:770	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	6	23	theme	epitopes	1322:1329	arg1	epitopes					1322:1329	AGP sugar epitopes	1312:1329	AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants	1312:1379	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	6	23	theme	epitopes	1322:1329	arg1	amounts					1301:1307	decreased amounts	1291:1307	decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants	1291:1379	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	3	24	theme	GLCAT14C	641:648	arg1	genes					650:654	the GLCAT14A, GLCAT14B, and GLCAT14C genes	613:654	the GLCAT14A, GLCAT14B, and GLCAT14C genes	613:654	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	1	25	theme	Arabinogalactan	107:121	arg1	AGPs					133:136	AGPs	133:136	AGPs	133:136	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	1	25	theme	Arabinogalactan	107:121	arg1	proteins					123:130	Arabinogalactan proteins	107:130	Arabinogalactan proteins (AGPs)	107:137	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	1	25	theme	Arabinogalactan	107:121	arg1	proteins					186:193	complex, hyperglycosylated plant cell wall proteins	143:193	complex, hyperglycosylated plant cell wall proteins	143:193	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	6	26	from	reductions	1163:1172	arg1	sugars					1181:1186	all sugars	1177:1186	all sugars in glcat14a/b	1177:1200	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	7	27	theme	Arabidopsis	1486:1496	arg1	reproduction					1505:1516	Arabidopsis sexual reproduction	1486:1516	Arabidopsis sexual reproduction	1486:1516	This work demonstrates the important roles that AG glucuronidation plays in Arabidopsis sexual reproduction and reproductive development.					
34726315	0	28	theme	II	24:25	arg1	polysaccharides					43:57	type II arabinogalactan polysaccharides	19:57	type II arabinogalactan polysaccharides	19:57	Glucuronidation of type II arabinogalactan polysaccharides function in sexual reproduction of Arabidopsis.					
34726315	3	29	theme	abnormal	731:738	arg1	development					740:750	abnormal development	731:750	abnormal development of the intine layer	731:770	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	5	30	theme	glcat14a/b/c	1022:1033	arg1	ovules					1035:1040	glcat14a/b/c ovules	1022:1040	glcat14a/b/c ovules	1022:1040	In addition, we observed excessive attraction of pollen tubes targeting glcat14a/b/c ovules, indicating that the polytubey block mechanism was compromised.					
34726315	0	31	theme	type	19:22	arg1	polysaccharides					43:57	type II arabinogalactan polysaccharides	19:57	type II arabinogalactan polysaccharides	19:57	Glucuronidation of type II arabinogalactan polysaccharides function in sexual reproduction of Arabidopsis.					
34726315	3	32	theme	exine	719:723	arg1	wall					725:728	the exine wall	715:728	the exine wall	715:728	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	33	theme	GLCAT14B	627:634	arg1	genes					650:654	the GLCAT14A, GLCAT14B, and GLCAT14C genes	613:654	the GLCAT14A, GLCAT14B, and GLCAT14C genes	613:654	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	6	34	theme	significant	1151:1161	arg1	reductions					1163:1172	significant reductions	1151:1172	significant reductions in all sugars in glcat14a/b	1151:1200	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	2	35	theme	residues	406:413	arg1	addition					378:385	the addition	374:385	the addition of glucuronic acid residues to AGPs	374:421	Here, we report that GLCAT14A, GLCAT14B, and GLCAT14C, three enzymes responsible for the addition of glucuronic acid residues to AGPs, function in pollen development, polytubey block, and normal embryo development in Arabidopsis.					
34726315	0	36	theme	polysaccharides	43:57	arg1	Glucuronidation					0:14	Glucuronidation	0:14	Glucuronidation of type II arabinogalactan polysaccharides	0:57	Glucuronidation of type II arabinogalactan polysaccharides function in sexual reproduction of Arabidopsis.					
34726315	3	37	theme	wall	725:728	arg1	structure					702:710	the reticulate structure	687:710	the reticulate structure of the exine wall	687:728	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	6	38	theme	glcat14a/b/c	1360:1371	arg1	mutants					1373:1379	glcat14a/b/c mutants	1360:1379	glcat14a/b/c mutants	1360:1379	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	6	39	theme	sugar	1316:1320	arg1	epitopes					1322:1329	AGP sugar epitopes	1312:1329	AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants	1312:1379	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	2	40	theme	acid	401:404	arg1	residues					406:413	glucuronic acid residues	390:413	glucuronic acid residues	390:413	Here, we report that GLCAT14A, GLCAT14B, and GLCAT14C, three enzymes responsible for the addition of glucuronic acid residues to AGPs, function in pollen development, polytubey block, and normal embryo development in Arabidopsis.					
34726315	0	41	theme	arabinogalactan	27:41	arg1	polysaccharides					43:57	type II arabinogalactan polysaccharides	19:57	type II arabinogalactan polysaccharides	19:57	Glucuronidation of type II arabinogalactan polysaccharides function in sexual reproduction of Arabidopsis.					
34726315	3	42	from	disorganization	668:682	arg1	glcat14a/b					806:815	glcat14a/b	806:815	glcat14a/b	806:815	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	42	from	disorganization	668:682	arg1	mutants					834:840	glcat14a/b/c mutants	821:840	glcat14a/b/c mutants	821:840	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	43	theme	GLCAT14A	617:624	arg1	genes					650:654	the GLCAT14A, GLCAT14B, and GLCAT14C genes	613:654	the GLCAT14A, GLCAT14B, and GLCAT14C genes	613:654	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	5	44	theme	pollen	999:1004	arg1	tubes					1006:1010	pollen tubes	999:1010	pollen tubes targeting glcat14a/b/c ovules	999:1040	In addition, we observed excessive attraction of pollen tubes targeting glcat14a/b/c ovules, indicating that the polytubey block mechanism was compromised.					
34726315	1	45	theme	biological	223:232	arg1	roles					234:238	the biological roles	219:238	the biological roles of their glycan moieties in sexual reproduction	219:286	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	3	46	theme	intine	759:764	arg1	layer					766:770	the intine layer	755:770	the intine layer	755:770	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	5	47	theme	block	1073:1077	arg1	mechanism					1079:1087	the polytubey block mechanism	1059:1087	the polytubey block mechanism	1059:1087	In addition, we observed excessive attraction of pollen tubes targeting glcat14a/b/c ovules, indicating that the polytubey block mechanism was compromised.					
34726315	5	48	theme	tubes	1006:1010	arg1	attraction					985:994	excessive attraction	975:994	excessive attraction of pollen tubes targeting glcat14a/b/c ovules	975:1040	In addition, we observed excessive attraction of pollen tubes targeting glcat14a/b/c ovules, indicating that the polytubey block mechanism was compromised.					
34726315	4	49	theme	same	894:897	arg1	anther					899:904	the same anther	890:904	the same anther	890:904	Synchronous development between locules within the same anther was also lost in some glcat14a/b/c stamens.					
34726315	2	50	theme	pollen	436:441	arg1	development					443:453	pollen development	436:453	pollen development	436:453	Here, we report that GLCAT14A, GLCAT14B, and GLCAT14C, three enzymes responsible for the addition of glucuronic acid residues to AGPs, function in pollen development, polytubey block, and normal embryo development in Arabidopsis.					
34726315	3	51	theme	genes	650:654	arg1	function					601:608	function	601:608	function of the GLCAT14A, GLCAT14B, and GLCAT14C genes	601:654	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	5	52	theme	polytubey	1063:1071	arg1	mechanism					1079:1087	the polytubey block mechanism	1059:1087	the polytubey block mechanism	1059:1087	In addition, we observed excessive attraction of pollen tubes targeting glcat14a/b/c ovules, indicating that the polytubey block mechanism was compromised.					
34726315	3	53	theme	glcat14a/b/c	821:832	arg1	mutants					834:840	glcat14a/b/c mutants	821:840	glcat14a/b/c mutants	821:840	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	6	54	theme	glcat14a/b/c	1206:1217	arg1	mutants					1219:1225	glcat14a/b/c mutants	1206:1225	glcat14a/b/c mutants	1206:1225	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	1	55	from	roles	234:238	arg1	reproduction					275:286	sexual reproduction	268:286	sexual reproduction	268:286	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	3	56	dep	biochemical	525:535	arg1	techniques					556:565	techniques	556:565	techniques	556:565	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	6	57	theme	Monosaccharide	1106:1119	arg1	analysis					1133:1140	Monosaccharide composition analysis	1106:1140	Monosaccharide composition analysis	1106:1140	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	1	58	with	proteins	186:193	arg1	little					200:205	little	200:205	little	200:205	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	3	59	theme	grains	796:801	arg1	collapse					777:784	collapse	777:784	collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants	777:840	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	59	theme	grains	796:801	arg1	disorganization					668:682	disorganization	668:682	disorganization of the reticulate structure of the exine wall	668:728	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	59	theme	grains	796:801	arg1	development					740:750	abnormal development	731:750	abnormal development of the intine layer	731:770	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	1	60	theme	complex	143:149	arg1	proteins					123:130	Arabinogalactan proteins	107:130	Arabinogalactan proteins (AGPs)	107:137	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	1	60	theme	complex	143:149	arg1	proteins					186:193	complex, hyperglycosylated plant cell wall proteins	143:193	complex, hyperglycosylated plant cell wall proteins	143:193	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
34726315	6	61	theme	composition	1121:1131	arg1	analysis					1133:1140	Monosaccharide composition analysis	1106:1140	Monosaccharide composition analysis	1106:1140	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	2	62	theme	normal	477:482	arg1	development					491:501	normal embryo development	477:501	normal embryo development	477:501	Here, we report that GLCAT14A, GLCAT14B, and GLCAT14C, three enzymes responsible for the addition of glucuronic acid residues to AGPs, function in pollen development, polytubey block, and normal embryo development in Arabidopsis.					
34726315	2	63	theme	glucuronic	390:399	arg1	residues					406:413	glucuronic acid residues	390:413	glucuronic acid residues	390:413	Here, we report that GLCAT14A, GLCAT14B, and GLCAT14C, three enzymes responsible for the addition of glucuronic acid residues to AGPs, function in pollen development, polytubey block, and normal embryo development in Arabidopsis.					
34726315	5	64	theme	excessive	975:983	arg1	attraction					985:994	excessive attraction	975:994	excessive attraction of pollen tubes targeting glcat14a/b/c ovules	975:1040	In addition, we observed excessive attraction of pollen tubes targeting glcat14a/b/c ovules, indicating that the polytubey block mechanism was compromised.					
34726315	2	65	theme	responsible	358:368	arg1	enzymes					350:356	three enzymes	344:356	three enzymes responsible for the addition of glucuronic acid residues to AGPs	344:421	Here, we report that GLCAT14A, GLCAT14B, and GLCAT14C, three enzymes responsible for the addition of glucuronic acid residues to AGPs, function in pollen development, polytubey block, and normal embryo development in Arabidopsis.					
34726315	3	66	theme	layer	766:770	arg1	collapse					777:784	collapse	777:784	collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants	777:840	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	66	theme	layer	766:770	arg1	disorganization					668:682	disorganization	668:682	disorganization of the reticulate structure of the exine wall	668:728	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	3	66	theme	layer	766:770	arg1	development					740:750	abnormal development	731:750	abnormal development of the intine layer	731:770	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	0	67	theme	sexual	71:76	arg1	reproduction					78:89	sexual reproduction	71:89	sexual reproduction of Arabidopsis	71:104	Glucuronidation of type II arabinogalactan polysaccharides function in sexual reproduction of Arabidopsis.					
34726315	3	68	theme	function	601:608	arg1	loss					593:596	the loss	589:596	the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes	589:654	Using biochemical and immunolabeling techniques, we demonstrated that the loss of function of the GLCAT14A, GLCAT14B, and GLCAT14C genes resulted in disorganization of the reticulate structure of the exine wall, abnormal development of the intine layer, and collapse of pollen grains in glcat14a/b and glcat14a/b/c mutants.					
34726315	4	69	theme	glcat14a/b/c	928:939	arg1	stamens					941:947	some glcat14a/b/c stamens	923:947	some glcat14a/b/c stamens	923:947	Synchronous development between locules within the same anther was also lost in some glcat14a/b/c stamens.					
34726315	6	70	from	mutants	1219:1225	arg1	sugars					1181:1186	all sugars	1177:1186	all sugars in glcat14a/b	1177:1200	Monosaccharide composition analysis revealed significant reductions in all sugars in glcat14a/b and glcat14a/b/c mutants except for arabinose and galactose, while immunolabeling showed decreased amounts of AGP sugar epitopes recognized by glcat14a/b and glcat14a/b/c mutants compared with the wild type.					
34726315	1	71	theme	glycan	249:254	arg1	moieties					256:263	their glycan moieties	243:263	their glycan moieties	243:263	Arabinogalactan proteins (AGPs) are complex, hyperglycosylated plant cell wall proteins with little known about the biological roles of their glycan moieties in sexual reproduction.					
33683232	7	0	theme	modest	1697:1702	arg1	reductions					1704:1713	only modest reductions	1692:1713	only modest reductions in emulsion quality	1692:1733	Replacing the liposome extrusion step with vortexing can reduce time and cost of bioreactor production with only modest reductions in emulsion quality.					
33683232	1	1	dep	interior	344:351	arg1	out					333:335	out	333:335	out	333:335	Vesicle-stabilized all-aqueous emulsion droplets are appealing as bioreactors because they provide uniform encapsulation via equilibrium partitioning without restricting diffusion in and out of the interior.					
33683232	2	2	theme	aqueous	402:408	arg1	ATPS					428:431	ATPS	428:431	ATPS	428:431	These properties rely on the composition of the aqueous two-phase system (ATPS) chosen for the emulsion and the structure of the interfacial liposome layer, respectively.					
33683232	2	2	theme	aqueous	402:408	arg1	system					420:425	the aqueous two-phase system	398:425	the aqueous two-phase system (ATPS) chosen for the emulsion	398:456	These properties rely on the composition of the aqueous two-phase system (ATPS) chosen for the emulsion and the structure of the interfacial liposome layer, respectively.					
33683232	4	3	theme	diameter	992:999	arg1	vesicles					965:972	vesicles	965:972	vesicles	965:972	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	3	4	dep	poly	600:603	arg1	systems					700:706	systems	700:706	systems	700:706	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	4	5	theme	gentle	917:922	arg1	extrusion					939:947	gentle hydration: (1) extrusion	917:947	gentle hydration: (1) extrusion	917:947	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	6	6	theme	potential	1563:1571	arg1	reactions					1573:1581	potential reactions	1563:1581	potential reactions	1563:1581	This work expands the compositions possible for liposome-stabilized, all-aqueous emulsion droplet bioreactors, making them amenable to a wider range of potential reactions.					
33683232	3	7	theme	two-phase	567:575	arg1	system					577:582	the aqueous two-phase system	555:582	the aqueous two-phase system	555:582	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	6	8	dep	liposome-stabilized	1459:1477	arg1	all-aqueous					1480:1490	all-aqueous	1480:1490	all-aqueous	1480:1490	This work expands the compositions possible for liposome-stabilized, all-aqueous emulsion droplet bioreactors, making them amenable to a wider range of potential reactions.					
33683232	6	9	theme	possible	1446:1453	arg1	compositions					1433:1444	the compositions	1429:1444	the compositions possible for liposome-stabilized, all-aqueous emulsion droplet bioreactors	1429:1519	This work expands the compositions possible for liposome-stabilized, all-aqueous emulsion droplet bioreactors, making them amenable to a wider range of potential reactions.					
33683232	4	10	theme	nm	1052:1053	arg1	diameter					1063:1070	270 nm average diameter	1048:1070	270 nm average diameter	1048:1070	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	5	11	from	partitioning	1340:1351	arg1	effective					1320:1328	effective	1320:1328	effective	1320:1328	Our data illustrate that while droplet uniformity and stability are somewhat better for samples based on extruded vesicles, extrusion is not necessary to create functional microreactors, as emulsions stabilized with vortexed liposomes are just as effective at solute partitioning and allow diffusion across the droplet's liposome corona.					
33683232	0	12	theme	aqueous	120:126	arg1	systems					137:143	aqueous biphasic systems	120:143	aqueous biphasic systems	120:143	Formation and properties of liposome-stabilized all-aqueous emulsions based on PEG/dextran, PEG/Ficoll, and PEG/sulfate aqueous biphasic systems.					
33683232	3	13	theme	droplet	716:722	arg1	uniformity					724:733	droplet uniformity	716:733	droplet uniformity	716:733	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	5	14	theme	extruded	1178:1185	arg1	vesicles					1187:1194	extruded vesicles	1178:1194	extruded vesicles	1178:1194	Our data illustrate that while droplet uniformity and stability are somewhat better for samples based on extruded vesicles, extrusion is not necessary to create functional microreactors, as emulsions stabilized with vortexed liposomes are just as effective at solute partitioning and allow diffusion across the droplet's liposome corona.					
33683232	4	15	theme	270	1048:1050	arg1	nm					1052:1053	nm	1052:1053	nm	1052:1053	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	0	16	theme	emulsions	60:68	arg1	properties					14:23	properties	14:23	properties	14:23	Formation and properties of liposome-stabilized all-aqueous emulsions based on PEG/dextran, PEG/Ficoll, and PEG/sulfate aqueous biphasic systems.					
33683232	0	16	theme	emulsions	60:68	arg1	Formation					0:8	Formation	0:8	Formation	0:8	Formation and properties of liposome-stabilized all-aqueous emulsions based on PEG/dextran, PEG/Ficoll, and PEG/sulfate aqueous biphasic systems.					
33683232	5	17	theme	liposome	1394:1401	arg1	corona					1403:1408	the droplet's liposome corona	1380:1408	the droplet's liposome corona	1380:1408	Our data illustrate that while droplet uniformity and stability are somewhat better for samples based on extruded vesicles, extrusion is not necessary to create functional microreactors, as emulsions stabilized with vortexed liposomes are just as effective at solute partitioning and allow diffusion across the droplet's liposome corona.					
33683232	5	18	theme	solute	1333:1338	arg1	partitioning					1340:1351	solute partitioning	1333:1351	solute partitioning	1333:1351	Our data illustrate that while droplet uniformity and stability are somewhat better for samples based on extruded vesicles, extrusion is not necessary to create functional microreactors, as emulsions stabilized with vortexed liposomes are just as effective at solute partitioning and allow diffusion across the droplet's liposome corona.					
33683232	3	19	theme	aqueous	559:565	arg1	system					577:582	the aqueous two-phase system	555:582	the aqueous two-phase system	555:582	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	4	20	theme	diameter	1063:1070	arg1	vesicles					1036:1043	vesicles	1036:1043	vesicles of 270 nm average diameter	1036:1070	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	4	21	theme	150	977:979	arg1	nm					981:982	nm	981:982	nm	981:982	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	4	22	theme	different	826:834	arg1	methods					836:842	two different methods	822:842	two different methods	822:842	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	4	23	dep	vortexing	1010:1018	arg1	2					1007:1007	2	1007:1007	2	1007:1007	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	3	24	theme	standard	591:598	arg1	ethyleneglycol					605:618	ethyleneglycol	605:618	ethyleneglycol	605:618	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	3	24	theme	standard	591:598	arg1	poly					600:603	a standard poly	589:603	a standard poly(ethyleneglycol)	589:619	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	4	25	theme	average	1055:1061	arg1	diameter					1063:1070	270 nm average diameter	1048:1070	270 nm average diameter	1048:1070	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	6	26	theme	liposome-stabilized	1459:1477	arg1	bioreactors					1509:1519	liposome-stabilized, all-aqueous emulsion droplet bioreactors	1459:1519	liposome-stabilized, all-aqueous emulsion droplet bioreactors	1459:1519	This work expands the compositions possible for liposome-stabilized, all-aqueous emulsion droplet bioreactors, making them amenable to a wider range of potential reactions.					
33683232	4	27	theme	average	984:990	arg1	diameter					992:999	150 nm average diameter	977:999	150 nm average diameter	977:999	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	7	28	theme	liposome	1598:1605	arg1	step					1617:1620	the liposome extrusion step	1594:1620	the liposome extrusion step	1594:1620	Replacing the liposome extrusion step with vortexing can reduce time and cost of bioreactor production with only modest reductions in emulsion quality.					
33683232	4	29	theme	polydisperse	885:896	arg1	vesicles					898:905	multilamellar, polydisperse vesicles	870:905	vesicles	898:905	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	7	30	theme	emulsion	1718:1725	arg1	quality					1727:1733	emulsion quality	1718:1733	emulsion quality	1718:1733	Replacing the liposome extrusion step with vortexing can reduce time and cost of bioreactor production with only modest reductions in emulsion quality.					
33683232	0	31	dep	PEG/dextran	79:89	arg1	systems					137:143	aqueous biphasic systems	120:143	aqueous biphasic systems	120:143	Formation and properties of liposome-stabilized all-aqueous emulsions based on PEG/dextran, PEG/Ficoll, and PEG/sulfate aqueous biphasic systems.					
33683232	4	32	theme	nm	981:982	arg1	diameter					992:999	150 nm average diameter	977:999	150 nm average diameter	977:999	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	5	33	theme	droplet	1104:1110	arg1	uniformity					1112:1121	droplet uniformity	1104:1121	droplet uniformity	1104:1121	Our data illustrate that while droplet uniformity and stability are somewhat better for samples based on extruded vesicles, extrusion is not necessary to create functional microreactors, as emulsions stabilized with vortexed liposomes are just as effective at solute partitioning and allow diffusion across the droplet's liposome corona.					
33683232	0	34	theme	biphasic	128:135	arg1	systems					137:143	aqueous biphasic systems	120:143	aqueous biphasic systems	120:143	Formation and properties of liposome-stabilized all-aqueous emulsions based on PEG/dextran, PEG/Ficoll, and PEG/sulfate aqueous biphasic systems.					
33683232	7	35	from	reductions	1704:1713	arg1	quality					1727:1733	emulsion quality	1718:1733	emulsion quality	1718:1733	Replacing the liposome extrusion step with vortexing can reduce time and cost of bioreactor production with only modest reductions in emulsion quality.					
33683232	5	36	theme	functional	1234:1243	arg1	microreactors					1245:1257	functional microreactors	1234:1257	functional microreactors	1234:1257	Our data illustrate that while droplet uniformity and stability are somewhat better for samples based on extruded vesicles, extrusion is not necessary to create functional microreactors, as emulsions stabilized with vortexed liposomes are just as effective at solute partitioning and allow diffusion across the droplet's liposome corona.					
33683232	1	37	theme	all-aqueous	165:175	arg1	droplets					186:193	Vesicle-stabilized all-aqueous emulsion droplets	146:193	Vesicle-stabilized all-aqueous emulsion droplets	146:193	Vesicle-stabilized all-aqueous emulsion droplets are appealing as bioreactors because they provide uniform encapsulation via equilibrium partitioning without restricting diffusion in and out of the interior.					
33683232	0	38	theme	all-aqueous	48:58	arg1	emulsions					60:68	liposome-stabilized all-aqueous emulsions	28:68	liposome-stabilized all-aqueous emulsions	28:68	Formation and properties of liposome-stabilized all-aqueous emulsions based on PEG/dextran, PEG/Ficoll, and PEG/sulfate aqueous biphasic systems.					
33683232	6	39	theme	emulsion	1492:1499	arg1	bioreactors					1509:1519	liposome-stabilized, all-aqueous emulsion droplet bioreactors	1459:1519	liposome-stabilized, all-aqueous emulsion droplet bioreactors	1459:1519	This work expands the compositions possible for liposome-stabilized, all-aqueous emulsion droplet bioreactors, making them amenable to a wider range of potential reactions.					
33683232	7	40	theme	extrusion	1607:1615	arg1	step					1617:1620	the liposome extrusion step	1594:1620	the liposome extrusion step	1594:1620	Replacing the liposome extrusion step with vortexing can reduce time and cost of bioreactor production with only modest reductions in emulsion quality.					
33683232	1	41	theme	uniform	245:251	arg1	encapsulation					253:265	uniform encapsulation	245:265	uniform encapsulation	245:265	Vesicle-stabilized all-aqueous emulsion droplets are appealing as bioreactors because they provide uniform encapsulation via equilibrium partitioning without restricting diffusion in and out of the interior.					
33683232	2	42	theme	layer	504:508	arg1	composition					383:393	the composition	379:393	the composition of the aqueous two-phase system (ATPS) chosen for the emulsion	379:456	These properties rely on the composition of the aqueous two-phase system (ATPS) chosen for the emulsion and the structure of the interfacial liposome layer, respectively.					
33683232	2	42	theme	layer	504:508	arg1	structure					466:474	the structure	462:474	the structure of the interfacial liposome layer, respectively	462:522	These properties rely on the composition of the aqueous two-phase system (ATPS) chosen for the emulsion and the structure of the interfacial liposome layer, respectively.					
33683232	3	43	theme	solute	763:768	arg1	partitioning					739:750	partitioning	739:750	partitioning of a model solute (U15 oligoRNA)	739:783	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	3	43	theme	solute	763:768	arg1	uniformity					724:733	droplet uniformity	716:733	droplet uniformity	716:733	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	0	44	theme	liposome-stabilized	28:46	arg1	emulsions					60:68	liposome-stabilized all-aqueous emulsions	28:68	liposome-stabilized all-aqueous emulsions	28:68	Formation and properties of liposome-stabilized all-aqueous emulsions based on PEG/dextran, PEG/Ficoll, and PEG/sulfate aqueous biphasic systems.					
33683232	3	45	theme	10	641:642	arg1	kDa					644:646	kDa	644:646	kDa	644:646	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	6	46	theme	wider	1548:1552	arg1	range					1554:1558	a wider range	1546:1558	a wider range of potential reactions	1546:1581	This work expands the compositions possible for liposome-stabilized, all-aqueous emulsion droplet bioreactors, making them amenable to a wider range of potential reactions.					
33683232	4	47	theme	hydration	924:932	arg1	extrusion					939:947	gentle hydration: (1) extrusion	917:947	gentle hydration: (1) extrusion	917:947	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	2	48	theme	liposome	495:502	arg1	layer					504:508	the interfacial liposome layer	479:508	the interfacial liposome layer	479:508	These properties rely on the composition of the aqueous two-phase system (ATPS) chosen for the emulsion and the structure of the interfacial liposome layer, respectively.					
33683232	4	49	dep	extrusion	939:947	arg1	1					936:936	1	936:936	1	936:936	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	3	50	theme	kDa	644:646	arg1	ATPS					648:651	8 kDa/dextran 10 kDa ATPS	627:651	8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa	627:678	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	2	51	theme	interfacial	483:493	arg1	layer					504:508	the interfacial liposome layer	479:508	the interfacial liposome layer	479:508	These properties rely on the composition of the aqueous two-phase system (ATPS) chosen for the emulsion and the structure of the interfacial liposome layer, respectively.					
33683232	3	52	theme	kDa/Ficoll	662:671	arg1	kDa					676:678	PEG 8 kDa/Ficoll 70 kDa	656:678	PEG 8 kDa/Ficoll 70 kDa	656:678	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	1	53	theme	emulsion	177:184	arg1	droplets					186:193	Vesicle-stabilized all-aqueous emulsion droplets	146:193	Vesicle-stabilized all-aqueous emulsion droplets	146:193	Vesicle-stabilized all-aqueous emulsion droplets are appealing as bioreactors because they provide uniform encapsulation via equilibrium partitioning without restricting diffusion in and out of the interior.					
33683232	6	54	theme	droplet	1501:1507	arg1	bioreactors					1509:1519	liposome-stabilized, all-aqueous emulsion droplet bioreactors	1459:1519	liposome-stabilized, all-aqueous emulsion droplet bioreactors	1459:1519	This work expands the compositions possible for liposome-stabilized, all-aqueous emulsion droplet bioreactors, making them amenable to a wider range of potential reactions.					
33683232	3	55	theme	model	757:761	arg1	solute					763:768	a model solute	755:768	a model solute (U15 oligoRNA)	755:783	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	3	55	theme	model	757:761	arg1	oligoRNA					775:782	U15 oligoRNA	771:782	U15 oligoRNA	771:782	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	4	56	theme	multilamellar	870:882	arg1	vesicles					898:905	multilamellar, polydisperse vesicles	870:905	vesicles	898:905	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	6	57	theme	reactions	1573:1581	arg1	range					1554:1558	a wider range	1546:1558	a wider range of potential reactions	1546:1581	This work expands the compositions possible for liposome-stabilized, all-aqueous emulsion droplet bioreactors, making them amenable to a wider range of potential reactions.					
33683232	7	58	theme	production	1676:1685	arg1	cost					1657:1660	cost	1657:1660	cost	1657:1660	Replacing the liposome extrusion step with vortexing can reduce time and cost of bioreactor production with only modest reductions in emulsion quality.					
33683232	7	58	theme	production	1676:1685	arg1	time					1648:1651	time	1648:1651	time	1648:1651	Replacing the liposome extrusion step with vortexing can reduce time and cost of bioreactor production with only modest reductions in emulsion quality.					
33683232	3	59	theme	PEG	656:658	arg1	kDa					676:678	PEG 8 kDa/Ficoll 70 kDa	656:678	PEG 8 kDa/Ficoll 70 kDa	656:678	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	3	60	theme	kDa/dextran	629:639	arg1	ATPS					648:651	8 kDa/dextran 10 kDa ATPS	627:651	8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa	627:678	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	1	61	theme	Vesicle-stabilized	146:163	arg1	droplets					186:193	Vesicle-stabilized all-aqueous emulsion droplets	146:193	Vesicle-stabilized all-aqueous emulsion droplets	146:193	Vesicle-stabilized all-aqueous emulsion droplets are appealing as bioreactors because they provide uniform encapsulation via equilibrium partitioning without restricting diffusion in and out of the interior.					
33683232	1	62	theme	equilibrium	271:281	arg1	partitioning					283:294	equilibrium partitioning	271:294	equilibrium partitioning	271:294	Vesicle-stabilized all-aqueous emulsion droplets are appealing as bioreactors because they provide uniform encapsulation via equilibrium partitioning without restricting diffusion in and out of the interior.					
33683232	5	63	theme	vortexed	1289:1296	arg1	liposomes					1298:1306	vortexed liposomes	1289:1306	vortexed liposomes	1289:1306	Our data illustrate that while droplet uniformity and stability are somewhat better for samples based on extruded vesicles, extrusion is not necessary to create functional microreactors, as emulsions stabilized with vortexed liposomes are just as effective at solute partitioning and allow diffusion across the droplet's liposome corona.					
33683232	2	64	theme	system	420:425	arg1	composition					383:393	the composition	379:393	the composition of the aqueous two-phase system (ATPS) chosen for the emulsion	379:456	These properties rely on the composition of the aqueous two-phase system (ATPS) chosen for the emulsion and the structure of the interfacial liposome layer, respectively.					
33683232	2	64	theme	system	420:425	arg1	structure					466:474	the structure	462:474	the structure of the interfacial liposome layer, respectively	462:522	These properties rely on the composition of the aqueous two-phase system (ATPS) chosen for the emulsion and the structure of the interfacial liposome layer, respectively.					
33683232	4	65	theme	vortexing	1010:1018	arg1	vesicles					965:972	vesicles	965:972	vesicles	965:972	We also compare liposomes formed by two different methods, both of which begin with multilamellar, polydisperse vesicles formed by gentle hydration: (1) extrusion, which produced vesicles of 150 nm average diameter, and (2) vortexing, which produced vesicles of 270 nm average diameter.					
33683232	3	66	theme	PEG	683:685	arg1	kDa/Na2SO4					689:698	PEG 8 kDa/Na2SO4	683:698	PEG 8 kDa/Na2SO4	683:698	Here, we explore how changing the aqueous two-phase system from a standard poly(ethyleneglycol), PEG, 8 kDa/dextran 10 kDa ATPS to PEG 8 kDa/Ficoll 70 kDa or PEG 8 kDa/Na2SO4 systems impacts droplet uniformity and partitioning of a model solute (U15 oligoRNA).					
33683232	2	67	theme	two-phase	410:418	arg1	ATPS					428:431	ATPS	428:431	ATPS	428:431	These properties rely on the composition of the aqueous two-phase system (ATPS) chosen for the emulsion and the structure of the interfacial liposome layer, respectively.					
33683232	2	67	theme	two-phase	410:418	arg1	system					420:425	the aqueous two-phase system	398:425	the aqueous two-phase system (ATPS) chosen for the emulsion	398:456	These properties rely on the composition of the aqueous two-phase system (ATPS) chosen for the emulsion and the structure of the interfacial liposome layer, respectively.					
33683232	7	68	theme	bioreactor	1665:1674	arg1	production					1676:1685	bioreactor production	1665:1685	bioreactor production	1665:1685	Replacing the liposome extrusion step with vortexing can reduce time and cost of bioreactor production with only modest reductions in emulsion quality.					
33571585	0	0	theme	selenium	90:97	arg1	biocompatibility					148:163	biocompatibility	148:163	biocompatibility	148:163	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	0	theme	selenium	90:97	arg1	antibiofilm					132:142	antibiofilm	132:142	antibiofilm	132:142	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	0	theme	selenium	90:97	arg1	Characterization					114:129	Characterization	114:129	Characterization	114:129	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	0	theme	selenium	90:97	arg1	nanoparticles					99:111	cellulose and myco-synthesized selenium nanoparticles	59:111	cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility	59:163	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	6	1	theme	cells	1281:1285	arg1	line					1257:1260	the normal cell line	1241:1260	the normal cell line of lung fibroblast cells (Wi-38)	1241:1293	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	3	2	with	mapping	661:667	arg1	EDX					629:631	EDX	629:631	EDX	629:631	Myco-synthesized Se-NPs and TC were fully characterized using UV, FT-IR, XRD, SEM with EDX, particle distribution, and mapping.					
33571585	0	3	theme	myco-synthesized	73:88	arg1	biocompatibility					148:163	biocompatibility	148:163	biocompatibility	148:163	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	3	theme	myco-synthesized	73:88	arg1	antibiofilm					132:142	antibiofilm	132:142	antibiofilm	132:142	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	3	theme	myco-synthesized	73:88	arg1	Characterization					114:129	Characterization	114:129	Characterization	114:129	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	3	theme	myco-synthesized	73:88	arg1	nanoparticles					99:111	cellulose and myco-synthesized selenium nanoparticles	59:111	cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility	59:163	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	6	4	theme	fibroblast	1270:1279	arg1	Wi-38					1288:1292	Wi-38	1288:1292	Wi-38	1288:1292	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	4	theme	fibroblast	1270:1279	arg1	cells					1281:1285	lung fibroblast cells	1265:1285	lung fibroblast cells (Wi-38)	1265:1293	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	5	5	theme	composite	937:945	arg1	nanoparticles					914:926	myco-synthesized novel cellulose-based selenium nanoparticles	866:926	myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite	866:945	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	1	6	theme	high	285:288	arg1	antimicrobial					299:311	high efficacy antimicrobial and anti-biofilm composites	285:339	antimicrobial	299:311	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	6	7	theme	shape	1335:1339	arg1	shrinking					1374:1382	shrinking	1374:1382	shrinking	1374:1382	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	7	theme	shape	1335:1339	arg1	loss					1308:1311	loss	1308:1311	loss of their typical cell shape	1308:1339	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	7	theme	shape	1335:1339	arg1	alteration					1218:1227	alteration	1218:1227	alteration occurred in the normal cell line of lung fibroblast cells (Wi-38)	1218:1293	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	7	theme	shape	1335:1339	arg1	granulation					1342:1352	granulation	1342:1352	granulation	1342:1352	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	7	theme	shape	1335:1339	arg1	loss					1355:1358	loss	1355:1358	loss of monolayer	1355:1371	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	7	theme	shape	1335:1339	arg1	rounding					1387:1394	rounding	1387:1394	rounding	1387:1394	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	8	theme	lung	1265:1268	arg1	Wi-38					1288:1292	Wi-38	1288:1292	Wi-38	1288:1292	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	8	theme	lung	1265:1268	arg1	cells					1281:1285	lung fibroblast cells	1265:1285	lung fibroblast cells (Wi-38)	1265:1293	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	9	theme	Se-NPs	1191:1196	arg1	cytotoxicity					1175:1186	cytotoxicity	1175:1186	cytotoxicity of Se-NPs and TC	1175:1203	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	10	theme	cell	1405:1408	arg1	shrinking					1374:1382	shrinking	1374:1382	shrinking	1374:1382	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	10	theme	cell	1405:1408	arg1	loss					1308:1311	loss	1308:1311	loss of their typical cell shape	1308:1339	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	10	theme	cell	1405:1408	arg1	alteration					1218:1227	alteration	1218:1227	alteration occurred in the normal cell line of lung fibroblast cells (Wi-38)	1218:1293	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	10	theme	cell	1405:1408	arg1	granulation					1342:1352	granulation	1342:1352	granulation	1342:1352	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	10	theme	cell	1405:1408	arg1	loss					1355:1358	loss	1355:1358	loss of monolayer	1355:1371	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	10	theme	cell	1405:1408	arg1	rounding					1387:1394	rounding	1387:1394	rounding	1387:1394	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	1	11	theme	efficacy	290:297	arg1	antimicrobial					299:311	high efficacy antimicrobial and anti-biofilm composites	285:339	antimicrobial	299:311	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	6	12	theme	cell	1330:1333	arg1	shape					1335:1339	their typical cell shape	1316:1339	their typical cell shape	1316:1339	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	5	13	theme	aureus	993:998	arg1	formation					962:970	the biofilm formation	950:970	the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans	950:1020	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	3	14	theme	Myco-synthesized	542:557	arg1	Se-NPs					559:564	Myco-synthesized Se-NPs	542:564	Myco-synthesized Se-NPs	542:564	Myco-synthesized Se-NPs and TC were fully characterized using UV, FT-IR, XRD, SEM with EDX, particle distribution, and mapping.					
33571585	1	15	theme	antimicrobial	299:311	arg1	synthesis					272:280	the synthesis	268:280	the synthesis of high efficacy antimicrobial and anti-biofilm composites	268:339	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	1	15	theme	antimicrobial	299:311	arg1	continuous					344:353	continuous	344:353	continuous	344:353	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	6	16	theme	typical	1322:1328	arg1	shape					1335:1339	their typical cell shape	1316:1339	their typical cell shape	1316:1339	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	17	theme	constant	1064:1071	arg1	inhibition					1081:1090	constant biofilm inhibition	1064:1090	constant biofilm inhibition	1064:1090	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	5	18	theme	novel	883:887	arg1	nanoparticles					914:926	myco-synthesized novel cellulose-based selenium nanoparticles	866:926	myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite	866:945	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	3	19	with	UV	604:605	arg1	EDX					629:631	EDX	629:631	EDX	629:631	Myco-synthesized Se-NPs and TC were fully characterized using UV, FT-IR, XRD, SEM with EDX, particle distribution, and mapping.					
33571585	6	20	theme	cell	1252:1255	arg1	line					1257:1260	the normal cell line	1241:1260	the normal cell line of lung fibroblast cells (Wi-38)	1241:1293	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	0	21	dep	nanoparticles	99:111	arg1	biocompatibility					148:163	biocompatibility	148:163	biocompatibility	148:163	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	21	dep	nanoparticles	99:111	arg1	antibiofilm					132:142	antibiofilm	132:142	antibiofilm	132:142	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	21	dep	nanoparticles	99:111	arg1	Characterization					114:129	Characterization	114:129	Characterization	114:129	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	21	dep	nanoparticles	99:111	arg1	nanoparticles					99:111	cellulose and myco-synthesized selenium nanoparticles	59:111	cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility	59:163	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	3	22	with	distribution	643:654	arg1	EDX					629:631	EDX	629:631	EDX	629:631	Myco-synthesized Se-NPs and TC were fully characterized using UV, FT-IR, XRD, SEM with EDX, particle distribution, and mapping.					
33571585	2	23	theme	tertiary	416:423	arg1	TC					436:437	TC	436:437	TC	436:437	In our study, a new and novel tertiary composite (TC) was synthesized, it composed of TEMPO cellulose (TOC), chitosan, starch, and myco-synthesized Se-NPs.					
33571585	2	23	theme	tertiary	416:423	arg1	composite					425:433	a new and novel tertiary composite	400:433	a new and novel tertiary composite (TC)	400:438	In our study, a new and novel tertiary composite (TC) was synthesized, it composed of TEMPO cellulose (TOC), chitosan, starch, and myco-synthesized Se-NPs.					
33571585	0	24	theme	novel	12:16	arg1	synthesis					18:26	Ecofriendly novel synthesis	0:26	Ecofriendly novel synthesis of tertiary composite	0:48	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	3	25	with	XRD	615:617	arg1	EDX					629:631	EDX	629:631	EDX	629:631	Myco-synthesized Se-NPs and TC were fully characterized using UV, FT-IR, XRD, SEM with EDX, particle distribution, and mapping.					
33571585	6	26	theme	normal	1245:1250	arg1	line					1257:1260	the normal cell line	1241:1260	the normal cell line of lung fibroblast cells (Wi-38)	1241:1293	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	27	with	cell	1405:1408	arg1	value					1423:1427	an IC50 value	1415:1427	an IC50 value of where 461 and 550 ppm respectively	1415:1465	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	4	28	theme	selenium	719:726	arg1	nanoparticles					728:740	selenium nanoparticles	719:740	selenium nanoparticles (Se-NPs)	719:749	The antimicrobial and anti-biofilm properties of selenium nanoparticles (Se-NPs) were effectively established for Pseudomonas aeruginosa and Staphylococcus aureus biofilms.					
33571585	4	28	theme	selenium	719:726	arg1	Se-NPs					743:748	Se-NPs	743:748	Se-NPs	743:748	The antimicrobial and anti-biofilm properties of selenium nanoparticles (Se-NPs) were effectively established for Pseudomonas aeruginosa and Staphylococcus aureus biofilms.					
33571585	2	29	theme	novel	410:414	arg1	TC					436:437	TC	436:437	TC	436:437	In our study, a new and novel tertiary composite (TC) was synthesized, it composed of TEMPO cellulose (TOC), chitosan, starch, and myco-synthesized Se-NPs.					
33571585	2	29	theme	novel	410:414	arg1	composite					425:433	a new and novel tertiary composite	400:433	a new and novel tertiary composite (TC)	400:438	In our study, a new and novel tertiary composite (TC) was synthesized, it composed of TEMPO cellulose (TOC), chitosan, starch, and myco-synthesized Se-NPs.					
33571585	6	30	theme	TC	1202:1203	arg1	cytotoxicity					1175:1186	cytotoxicity	1175:1186	cytotoxicity of Se-NPs and TC	1175:1203	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	5	31	theme	cellulose-based	889:903	arg1	nanoparticles					914:926	myco-synthesized novel cellulose-based selenium nanoparticles	866:926	myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite	866:945	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	6	32	theme	monolayer	1363:1371	arg1	shrinking					1374:1382	shrinking	1374:1382	shrinking	1374:1382	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	32	theme	monolayer	1363:1371	arg1	loss					1308:1311	loss	1308:1311	loss of their typical cell shape	1308:1339	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	32	theme	monolayer	1363:1371	arg1	alteration					1218:1227	alteration	1218:1227	alteration occurred in the normal cell line of lung fibroblast cells (Wi-38)	1218:1293	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	32	theme	monolayer	1363:1371	arg1	granulation					1342:1352	granulation	1342:1352	granulation	1342:1352	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	32	theme	monolayer	1363:1371	arg1	loss					1355:1358	loss	1355:1358	loss of monolayer	1355:1371	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	32	theme	monolayer	1363:1371	arg1	rounding					1387:1394	rounding	1387:1394	rounding	1387:1394	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	1	33	theme	anti-biofilm	317:328	arg1	composites					330:339	high efficacy antimicrobial and anti-biofilm composites	285:339	composites	330:339	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	5	34	theme	aeruginosa	978:987	arg1	formation					962:970	the biofilm formation	950:970	the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans	950:1020	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	3	35	with	SEM	620:622	arg1	EDX					629:631	EDX	629:631	EDX	629:631	Myco-synthesized Se-NPs and TC were fully characterized using UV, FT-IR, XRD, SEM with EDX, particle distribution, and mapping.					
33571585	6	36	theme	biofilm	1073:1079	arg1	inhibition					1081:1090	constant biofilm inhibition	1064:1090	constant biofilm inhibition	1064:1090	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	6	37	theme	Wi-38	1399:1403	arg1	cell					1405:1408	Wi-38 cell	1399:1408	Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively	1399:1465	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
33571585	1	38	theme	composites	330:339	arg1	synthesis					272:280	the synthesis	268:280	the synthesis of high efficacy antimicrobial and anti-biofilm composites	268:339	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	1	38	theme	composites	330:339	arg1	continuous					344:353	continuous	344:353	continuous	344:353	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	4	39	theme	nanoparticles	728:740	arg1	properties					705:714	The antimicrobial and anti-biofilm properties	670:714	properties	705:714	The antimicrobial and anti-biofilm properties of selenium nanoparticles (Se-NPs) were effectively established for Pseudomonas aeruginosa and Staphylococcus aureus biofilms.					
33571585	1	40	theme	Microbial	166:174	arg1	infections					176:185	Microbial infections	166:185	Microbial infections	166:185	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	0	41	theme	composite	40:48	arg1	synthesis					18:26	Ecofriendly novel synthesis	0:26	Ecofriendly novel synthesis of tertiary composite	0:48	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	5	42	theme	possible	847:854	arg1	impact					856:861	The possible impact	843:861	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans	843:1020	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	1	43	theme	other	240:244	arg1	infections					246:255	other infections	240:255	other infections	240:255	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	0	44	theme	tertiary	31:38	arg1	composite					40:48	tertiary composite	31:48	tertiary composite	31:48	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	2	45	theme	myco-synthesized	517:532	arg1	Se-NPs					534:539	myco-synthesized Se-NPs	517:539	myco-synthesized Se-NPs	517:539	In our study, a new and novel tertiary composite (TC) was synthesized, it composed of TEMPO cellulose (TOC), chitosan, starch, and myco-synthesized Se-NPs.					
33571585	2	46	theme	TEMPO	472:476	arg1	TOC					489:491	TOC	489:491	TOC	489:491	In our study, a new and novel tertiary composite (TC) was synthesized, it composed of TEMPO cellulose (TOC), chitosan, starch, and myco-synthesized Se-NPs.					
33571585	2	46	theme	TEMPO	472:476	arg1	cellulose					478:486	TEMPO cellulose	472:486	TEMPO cellulose (TOC)	472:492	In our study, a new and novel tertiary composite (TC) was synthesized, it composed of TEMPO cellulose (TOC), chitosan, starch, and myco-synthesized Se-NPs.					
33571585	5	47	theme	selenium	905:912	arg1	nanoparticles					914:926	myco-synthesized novel cellulose-based selenium nanoparticles	866:926	myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite	866:945	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	3	48	with	FT-IR	608:612	arg1	EDX					629:631	EDX	629:631	EDX	629:631	Myco-synthesized Se-NPs and TC were fully characterized using UV, FT-IR, XRD, SEM with EDX, particle distribution, and mapping.					
33571585	5	49	from	impact	856:861	arg1	formation					962:970	the biofilm formation	950:970	the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans	950:1020	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	1	50	theme	fight	358:362	arg1	resistance					374:383	fight microbial resistance	358:383	fight microbial resistance	358:383	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	4	51	theme	aureus	826:831	arg1	biofilms					833:840	Pseudomonas aeruginosa and Staphylococcus aureus biofilms	784:840	Pseudomonas aeruginosa and Staphylococcus aureus biofilms	784:840	The antimicrobial and anti-biofilm properties of selenium nanoparticles (Se-NPs) were effectively established for Pseudomonas aeruginosa and Staphylococcus aureus biofilms.					
33571585	4	52	theme	antimicrobial	674:686	arg1	properties					705:714	The antimicrobial and anti-biofilm properties	670:714	properties	705:714	The antimicrobial and anti-biofilm properties of selenium nanoparticles (Se-NPs) were effectively established for Pseudomonas aeruginosa and Staphylococcus aureus biofilms.					
33571585	1	53	theme	microbial	364:372	arg1	resistance					374:383	fight microbial resistance	358:383	fight microbial resistance	358:383	Microbial infections are considered common and dangerous for humans among other infections; therefore the synthesis of high efficacy antimicrobial and anti-biofilm composites is continuous to fight microbial resistance.					
33571585	5	54	theme	Candida	1005:1011	arg1	albicans					1013:1020	Candida albicans	1005:1020	Candida albicans	1005:1020	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	5	55	theme	biofilm	954:960	arg1	formation					962:970	the biofilm formation	950:970	the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans	950:1020	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	5	56	theme	albicans	1013:1020	arg1	formation					962:970	the biofilm formation	950:970	the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans	950:1020	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	4	57	theme	anti-biofilm	692:703	arg1	properties					705:714	The antimicrobial and anti-biofilm properties	670:714	properties	705:714	The antimicrobial and anti-biofilm properties of selenium nanoparticles (Se-NPs) were effectively established for Pseudomonas aeruginosa and Staphylococcus aureus biofilms.					
33571585	3	58	theme	particle	634:641	arg1	distribution					643:654	particle distribution	634:654	particle distribution	634:654	Myco-synthesized Se-NPs and TC were fully characterized using UV, FT-IR, XRD, SEM with EDX, particle distribution, and mapping.					
33571585	4	59	theme	aeruginosa	796:805	arg1	biofilms					833:840	Pseudomonas aeruginosa and Staphylococcus aureus biofilms	784:840	Pseudomonas aeruginosa and Staphylococcus aureus biofilms	784:840	The antimicrobial and anti-biofilm properties of selenium nanoparticles (Se-NPs) were effectively established for Pseudomonas aeruginosa and Staphylococcus aureus biofilms.					
33571585	2	60	theme	new	402:404	arg1	TC					436:437	TC	436:437	TC	436:437	In our study, a new and novel tertiary composite (TC) was synthesized, it composed of TEMPO cellulose (TOC), chitosan, starch, and myco-synthesized Se-NPs.					
33571585	2	60	theme	new	402:404	arg1	composite					425:433	a new and novel tertiary composite	400:433	a new and novel tertiary composite (TC)	400:438	In our study, a new and novel tertiary composite (TC) was synthesized, it composed of TEMPO cellulose (TOC), chitosan, starch, and myco-synthesized Se-NPs.					
33571585	5	61	theme	myco-synthesized	866:881	arg1	nanoparticles					914:926	myco-synthesized novel cellulose-based selenium nanoparticles	866:926	myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite	866:945	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	0	62	theme	cellulose	59:67	arg1	biocompatibility					148:163	biocompatibility	148:163	biocompatibility	148:163	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	62	theme	cellulose	59:67	arg1	antibiofilm					132:142	antibiofilm	132:142	antibiofilm	132:142	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	62	theme	cellulose	59:67	arg1	Characterization					114:129	Characterization	114:129	Characterization	114:129	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	0	62	theme	cellulose	59:67	arg1	nanoparticles					99:111	cellulose and myco-synthesized selenium nanoparticles	59:111	cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility	59:163	Ecofriendly novel synthesis of tertiary composite based on cellulose and myco-synthesized selenium nanoparticles: Characterization, antibiofilm and biocompatibility.					
33571585	5	63	theme	nanoparticles	914:926	arg1	impact					856:861	The possible impact	843:861	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans	843:1020	The possible impact of myco-synthesized novel cellulose-based selenium nanoparticles tertiary composite on the biofilm formation of P. aeruginosa, S. aureus, and Candida albicans was evaluated in this study.					
33571585	6	64	theme	IC50	1418:1421	arg1	value					1423:1427	an IC50 value	1415:1427	an IC50 value of where 461 and 550 ppm respectively	1415:1465	TC exhibited constant biofilm inhibition against P. aeruginosa, S. aureus, and C. albicans, while the results obtained from cytotoxicity of Se-NPs and TC showed that, alteration occurred in the normal cell line of lung fibroblast cells (Wi-38) was shown as loss of their typical cell shape, granulation, loss of monolayer, shrinking or rounding of Wi-38 cell with an IC50 value of where 461 and 550 ppm respectively.					
32031789	5	0	theme	piece-hot-press	841:855	arg1	BC					857:858	piece-hot-press BC	841:858	piece-hot-press BC	841:858	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	6	1	from	material	988:995	arg1	attractive					963:972	attractive	963:972	attractive	963:972	Thus, BC-laminated composites will be attractive in structural material.					
32031789	2	2	theme	cellulose	437:445	arg1	hydrogels					452:460	stack-up bacterial cellulose (BC) hydrogels	418:460	stack-up bacterial cellulose (BC) hydrogels	418:460	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	5	3	theme	tensile	821:827	arg1	strength					829:836	tensile strength	821:836	tensile strength	821:836	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	0	4	theme	Self-Aggregation	89:104	arg1	Forces					106:111	Self-Aggregation Forces	89:111	Self-Aggregation Forces	89:111	Robust All-Cellulose Nanofiber Composite from Stack-Up Bacterial Cellulose Hydrogels via Self-Aggregation Forces.					
32031789	2	5	theme	bacterial	427:435	arg1	hydrogels					452:460	stack-up bacterial cellulose (BC) hydrogels	418:460	stack-up bacterial cellulose (BC) hydrogels	418:460	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	5	6	theme	shear	802:806	arg1	strength					808:815	interfacial shear strength	790:815	interfacial shear strength	790:815	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	2	7	theme	environment-friendly	335:354	arg1	approach					356:363	an environment-friendly approach	332:363	an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing	332:535	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	4	8	theme	nanofibers	696:705	arg1	structure					679:687	the structure	675:687	the structure of all nanofibers	675:705	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	4	8	theme	nanofibers	696:705	arg1	strength					718:725	a tensile strength	708:725	a tensile strength of 116 MPa	708:736	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	4	8	theme	nanofibers	696:705	arg1	modulus					753:759	a storage modulus	743:759	a storage modulus of 25 GPa	743:769	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	4	9	contain	possess	667:673	arg2	strength					718:725	a tensile strength	708:725	a tensile strength of 116 MPa	708:736	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	4	9	contain	possess	667:673	arg1	composites					656:665	BC composites	653:665	BC composites	653:665	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	4	9	contain	possess	667:673	arg2	modulus					753:759	a storage modulus	743:759	a storage modulus of 25 GPa	743:769	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	4	9	contain	possess	667:673	arg2	structure					679:687	the structure	675:687	the structure of all nanofibers	675:705	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	3	10	theme	BC-laminated	578:589	arg1	composites					591:600	BC-laminated composites	578:600	BC-laminated composites	578:600	Structural and mechanical properties of BC-laminated composites have been investigated.					
32031789	1	11	theme	surface-selective	195:211	arg1	approach					225:232	a surface-selective dissolution approach	193:232	a surface-selective dissolution approach	193:232	All-cellulose composites are usually prepared by removing impurities and using a surface-selective dissolution approach, which detract significantly from their environment-friendly properties.					
32031789	5	12	theme	self-aggregation	883:898	arg1	forces					900:905	the strong self-aggregation forces	872:905	the strong self-aggregation forces of BC nanofibers	872:922	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	6	13	theme	structural	977:986	arg1	material					988:995	structural material	977:995	structural material	977:995	Thus, BC-laminated composites will be attractive in structural material.					
32031789	1	14	theme	dissolution	213:223	arg1	approach					225:232	a surface-selective dissolution approach	193:232	a surface-selective dissolution approach	193:232	All-cellulose composites are usually prepared by removing impurities and using a surface-selective dissolution approach, which detract significantly from their environment-friendly properties.					
32031789	5	15	theme	BC	857:858	arg1	strength					808:815	interfacial shear strength	790:815	interfacial shear strength	790:815	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	5	15	theme	BC	857:858	arg1	strength					829:836	tensile strength	821:836	tensile strength	821:836	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	2	16	theme	all-cellulose	378:390	arg1	composites					402:411	all-cellulose nanofiber composites	378:411	all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels	378:460	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	2	17	theme	water-based	514:524	arg1	processing					526:535	water-based processing	514:535	water-based processing	514:535	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	1	18	theme	All-cellulose	114:126	arg1	composites					128:137	All-cellulose composites	114:137	All-cellulose composites	114:137	All-cellulose composites are usually prepared by removing impurities and using a surface-selective dissolution approach, which detract significantly from their environment-friendly properties.					
32031789	4	19	theme	storage	745:751	arg1	modulus					753:759	a storage modulus	743:759	a storage modulus of 25 GPa	743:769	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	5	20	theme	BC	910:911	arg1	nanofibers					913:922	BC nanofibers	910:922	BC nanofibers	910:922	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	4	21	theme	BC	653:654	arg1	composites					656:665	BC composites	653:665	BC composites	653:665	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	2	22	theme	hydrogen	497:504	arg1	bond					506:509	the hydrogen bond	493:509	the hydrogen bond by water-based processing	493:535	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	2	23	theme	bond	506:509	arg1	forces					483:488	self-aggregation forces	466:488	self-aggregation forces of the hydrogen bond by water-based processing	466:535	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	4	24	theme	GPa	767:769	arg1	structure					679:687	the structure	675:687	the structure of all nanofibers	675:705	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	4	24	theme	GPa	767:769	arg1	strength					718:725	a tensile strength	708:725	a tensile strength of 116 MPa	708:736	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	4	24	theme	GPa	767:769	arg1	modulus					753:759	a storage modulus	743:759	a storage modulus of 25 GPa	743:769	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	3	25	theme	mechanical	553:562	arg1	properties					564:573	Structural and mechanical properties	538:573	Structural and mechanical properties of BC-laminated composites	538:600	Structural and mechanical properties of BC-laminated composites have been investigated.					
32031789	0	26	theme	Stack-Up	46:53	arg1	Hydrogels					75:83	Stack-Up Bacterial Cellulose Hydrogels	46:83	Stack-Up Bacterial Cellulose Hydrogels	46:83	Robust All-Cellulose Nanofiber Composite from Stack-Up Bacterial Cellulose Hydrogels via Self-Aggregation Forces.					
32031789	2	27	theme	self-aggregation	466:481	arg1	forces					483:488	self-aggregation forces	466:488	self-aggregation forces of the hydrogen bond by water-based processing	466:535	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	5	28	theme	interfacial	790:800	arg1	strength					808:815	interfacial shear strength	790:815	interfacial shear strength	790:815	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	6	29	from	attractive	963:972	arg1	material					988:995	structural material	977:995	structural material	977:995	Thus, BC-laminated composites will be attractive in structural material.					
32031789	2	30	from	hydrogels	452:460	arg1	composites					402:411	all-cellulose nanofiber composites	378:411	all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels	378:460	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	3	31	theme	Structural	538:547	arg1	properties					564:573	Structural and mechanical properties	538:573	Structural and mechanical properties of BC-laminated composites	538:600	Structural and mechanical properties of BC-laminated composites have been investigated.					
32031789	0	32	theme	Cellulose	65:73	arg1	Hydrogels					75:83	Stack-Up Bacterial Cellulose Hydrogels	46:83	Stack-Up Bacterial Cellulose Hydrogels	46:83	Robust All-Cellulose Nanofiber Composite from Stack-Up Bacterial Cellulose Hydrogels via Self-Aggregation Forces.					
32031789	0	33	from	Hydrogels	75:83	arg1	Composite					31:39	Composite	31:39	Composite	31:39	Robust All-Cellulose Nanofiber Composite from Stack-Up Bacterial Cellulose Hydrogels via Self-Aggregation Forces.					
32031789	2	34	theme	stack-up	418:425	arg1	hydrogels					452:460	stack-up bacterial cellulose (BC) hydrogels	418:460	stack-up bacterial cellulose (BC) hydrogels	418:460	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	0	35	theme	Bacterial	55:63	arg1	Hydrogels					75:83	Stack-Up Bacterial Cellulose Hydrogels	46:83	Stack-Up Bacterial Cellulose Hydrogels	46:83	Robust All-Cellulose Nanofiber Composite from Stack-Up Bacterial Cellulose Hydrogels via Self-Aggregation Forces.					
32031789	6	36	theme	BC-laminated	931:942	arg1	composites					944:953	BC-laminated composites	931:953	BC-laminated composites	931:953	Thus, BC-laminated composites will be attractive in structural material.					
32031789	3	37	theme	composites	591:600	arg1	properties					564:573	Structural and mechanical properties	538:573	Structural and mechanical properties of BC-laminated composites	538:600	Structural and mechanical properties of BC-laminated composites have been investigated.					
32031789	5	38	dep	strength	808:815	arg1	the					786:788	the	786:788	the	786:788	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	2	39	theme	nanofiber	392:400	arg1	composites					402:411	all-cellulose nanofiber composites	378:411	all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels	378:460	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	1	40	theme	environment-friendly	274:293	arg1	properties					295:304	their environment-friendly properties	268:304	their environment-friendly properties	268:304	All-cellulose composites are usually prepared by removing impurities and using a surface-selective dissolution approach, which detract significantly from their environment-friendly properties.					
32031789	4	41	theme	MPa	734:736	arg1	structure					679:687	the structure	675:687	the structure of all nanofibers	675:705	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	4	41	theme	MPa	734:736	arg1	strength					718:725	a tensile strength	708:725	a tensile strength of 116 MPa	708:736	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	4	41	theme	MPa	734:736	arg1	modulus					753:759	a storage modulus	743:759	a storage modulus of 25 GPa	743:769	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32031789	2	42	theme	BC	448:449	arg1	hydrogels					452:460	stack-up bacterial cellulose (BC) hydrogels	418:460	stack-up bacterial cellulose (BC) hydrogels	418:460	In this paper, we report an environment-friendly approach to fabricate all-cellulose nanofiber composites from stack-up bacterial cellulose (BC) hydrogels via self-aggregation forces of the hydrogen bond by water-based processing.					
32031789	5	43	theme	strong	876:881	arg1	forces					900:905	the strong self-aggregation forces	872:905	the strong self-aggregation forces of BC nanofibers	872:922	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	5	44	theme	nanofibers	913:922	arg1	forces					900:905	the strong self-aggregation forces	872:905	the strong self-aggregation forces of BC nanofibers	872:922	Additionally, the interfacial shear strength and tensile strength of piece-hot-press BC demonstrate the strong self-aggregation forces of BC nanofibers.					
32031789	4	45	theme	tensile	710:716	arg1	strength					718:725	a tensile strength	708:725	a tensile strength of 116 MPa	708:736	The results indicated that BC composites possess the structure of all nanofibers, a tensile strength of 116 MPa, and a storage modulus of 25 GPa.					
32573600	0	0	theme	alginate	98:105	arg1	composites					107:116	bioglass and alginate composites	85:116	bioglass and alginate composites	85:116	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.					
32573600	10	1	theme	cartilage	1617:1625	arg1	regeneration					1538:1549	the regeneration	1534:1549	the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments	1534:1639	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	6	2	theme	reduced	905:911	arg1	swelling					913:920	swelling	913:920	swelling	913:920	The results indicated that reduced swelling and degradation enhanced apatite formation.					
32573600	4	3	theme	chemical	730:737	arg1	interactions					739:750	the chemical interactions	726:750	the chemical interactions of the composites	726:768	Repetitive peaks from acids along with stretching confirmed the chemical interactions of the composites.					
32573600	2	4	theme	composite	393:401	arg1	scaffolds					412:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	6	5	theme	apatite	947:953	arg1	formation					955:963	apatite formation	947:963	apatite formation	947:963	The results indicated that reduced swelling and degradation enhanced apatite formation.					
32573600	2	6	theme	nBG	362:364	arg1	scaffolds					412:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	9	7	theme	profound	1285:1292	arg1	dynamism					1294:1301	profound dynamism	1285:1301	profound dynamism required to establish organoids of interest	1285:1345	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	10	8	theme	ligaments	1631:1639	arg1	regeneration					1538:1549	the regeneration	1534:1549	the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments	1534:1639	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	9	9	with	co-cultures	1177:1187	arg1	cells					1214:1218	KB3-1 cells	1208:1218	KB3-1 cells	1208:1218	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	9	9	with	co-cultures	1177:1187	arg1	cells					1198:1202	HDF cells	1194:1202	HDF cells	1194:1202	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	0	10	theme	composites	107:116	arg1	scaffolds					72:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds	0:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.	0:154	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.					
32573600	10	11	theme	infinite	1420:1427	arg1	possibilities					1429:1441	infinite possibilities	1420:1441	infinite possibilities	1420:1441	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	10	11	theme	infinite	1420:1427	arg1	matrix					1510:1515	an extracellular matrix	1493:1515	an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments	1493:1639	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	10	12	theme	medicine	1472:1479	arg1	field					1450:1454	the field	1446:1454	the field of regenerative medicine	1446:1479	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	10	13	theme	3D	1386:1387	arg1	membranes					1398:1406	these 3D hydrogel membranes	1380:1406	these 3D hydrogel membranes	1380:1406	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	0	14	theme	regenerative	129:140	arg1	applications					142:153	tissue regenerative applications	122:153	tissue regenerative applications	122:153	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.					
32573600	10	15	theme	extracellular	1496:1508	arg1	possibilities					1429:1441	infinite possibilities	1420:1441	infinite possibilities	1420:1441	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	10	15	theme	extracellular	1496:1508	arg1	ECM					1518:1520	ECM	1518:1520	ECM	1518:1520	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	10	15	theme	extracellular	1496:1508	arg1	matrix					1510:1515	an extracellular matrix	1493:1515	an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments	1493:1639	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	10	16	theme	bone	1554:1557	arg1	regeneration					1538:1549	the regeneration	1534:1549	the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments	1534:1639	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	1	17	from	increase	173:180	arg1	diseases					228:235	associated geriatric diseases	207:235	associated geriatric diseases	207:235	With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems.					
32573600	1	17	from	increase	173:180	arg1	populace					194:201	the aged populace	185:201	the aged populace	185:201	With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems.					
32573600	10	18	dep	such	1609:1612	arg1	as					1614:1615	as	1614:1615	as	1614:1615	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	10	19	theme	hydrogel	1389:1396	arg1	membranes					1398:1406	these 3D hydrogel membranes	1380:1406	these 3D hydrogel membranes	1380:1406	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	1	20	theme	aged	189:192	arg1	populace					194:201	the aged populace	185:201	the aged populace	185:201	With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems.					
32573600	0	21	theme	tissue	122:127	arg1	applications					142:153	tissue regenerative applications	122:153	tissue regenerative applications	122:153	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.					
32573600	2	22	theme	sol-gel	443:449	arg1	method					474:479	a sol-gel assisted freeze-drying method	441:479	a sol-gel assisted freeze-drying method	441:479	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	4	23	from	acids	688:692	arg1	peaks					677:681	Repetitive peaks	666:681	Repetitive peaks from acids along with stretching	666:714	Repetitive peaks from acids along with stretching confirmed the chemical interactions of the composites.					
32573600	2	24	theme	regenerative	532:543	arg1	medicine					545:552	regenerative medicine	532:552	regenerative medicine	532:552	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	2	25	theme	assisted	451:458	arg1	method					474:479	a sol-gel assisted freeze-drying method	441:479	a sol-gel assisted freeze-drying method	441:479	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	5	26	theme	in	803:804	arg1	degradation					812:822	degradation	812:822	degradation	812:822	Swelling ability, porosity, and in vitro degradation and biomineralization were analysed for up to 7 days.					
32573600	7	27	theme	hemostatic	992:1001	arg1	properties					1003:1012	the hemostatic properties	988:1012	the hemostatic properties on scaffolds	988:1025	Hemocompatibility and the hemostatic properties on scaffolds were also systematically investigated.					
32573600	3	28	theme	crystalline	591:601	arg1	structure					603:611	the combeite highly crystalline structure	571:611	the combeite highly crystalline structure of nBG and Alg amorphous broad peaks	571:648	The presence of the combeite highly crystalline structure of nBG and Alg amorphous broad peaks were confirmed.					
32573600	9	29	theme	3-D	1173:1175	arg1	co-cultures					1177:1187	Further 3-D co-cultures	1165:1187	Further 3-D co-cultures with HDF cells and KB3-1 cells	1165:1218	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	0	30	theme	Bioactive	0:8	arg1	scaffolds					72:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds	0:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.	0:154	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.					
32573600	1	31	theme	degenerated	288:298	arg1	systems					306:312	degenerated organ systems	288:312	degenerated organ systems	288:312	With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems.					
32573600	9	32	theme	Further	1165:1171	arg1	co-cultures					1177:1187	Further 3-D co-cultures	1165:1187	Further 3-D co-cultures with HDF cells and KB3-1 cells	1165:1218	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	3	33	theme	structure	603:611	arg1	presence					559:566	The presence	555:566	The presence of the combeite highly crystalline structure of nBG and Alg amorphous broad peaks	555:648	The presence of the combeite highly crystalline structure of nBG and Alg amorphous broad peaks were confirmed.					
32573600	0	34	theme	membranous	61:70	arg1	scaffolds					72:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds	0:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.	0:154	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.					
32573600	1	35	theme	associated	207:216	arg1	diseases					228:235	associated geriatric diseases	207:235	associated geriatric diseases	207:235	With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems.					
32573600	1	36	theme	organ	300:304	arg1	systems					306:312	degenerated organ systems	288:312	degenerated organ systems	288:312	With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems.					
32573600	8	37	theme	significant	1080:1090	arg1	cyto-compatibility					1092:1109	cyto-compatibility	1092:1109	cyto-compatibility	1092:1109	Additionally, significant cyto-compatibility and proliferation were noted in a culture with KB3-1.					
32573600	3	38	theme	combeite	575:582	arg1	structure					603:611	the combeite highly crystalline structure	571:611	the combeite highly crystalline structure of nBG and Alg amorphous broad peaks	571:648	The presence of the combeite highly crystalline structure of nBG and Alg amorphous broad peaks were confirmed.					
32573600	8	39	with	culture	1145:1151	arg1	KB3-1					1158:1162	KB3-1	1158:1162	KB3-1	1158:1162	Additionally, significant cyto-compatibility and proliferation were noted in a culture with KB3-1.					
32573600	1	40	theme	geriatric	218:226	arg1	diseases					228:235	associated geriatric diseases	207:235	associated geriatric diseases	207:235	With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems.					
32573600	0	41	theme	degradable	11:20	arg1	scaffolds					72:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds	0:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.	0:154	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.					
32573600	1	42	theme	systems	306:312	arg1	regeneration					272:283	the regeneration	268:283	the regeneration of degenerated organ systems	268:312	With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems.					
32573600	5	43	dep	in	803:804	arg1	vitro					806:810	vitro	806:810	vitro	806:810	Swelling ability, porosity, and in vitro degradation and biomineralization were analysed for up to 7 days.					
32573600	9	44	theme	HDF	1194:1196	arg1	cells					1198:1202	HDF cells	1194:1202	HDF cells	1194:1202	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	7	45	from	properties	1003:1012	arg1	scaffolds					1017:1025	scaffolds	1017:1025	scaffolds	1017:1025	Hemocompatibility and the hemostatic properties on scaffolds were also systematically investigated.					
32573600	0	46	theme	three-dimensional	43:59	arg1	scaffolds					72:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds	0:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.	0:154	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.					
32573600	10	47	theme	intra-vascularization	1560:1580	arg1	regeneration					1538:1549	the regeneration	1534:1549	the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments	1534:1639	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	9	48	from	formation	1239:1247	arg1	nBG-Zr/Alg					1269:1278	nBG-Zr/Alg	1269:1278	nBG-Zr/Alg	1269:1278	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	9	48	from	formation	1239:1247	arg1	nBG/Alg					1257:1263	nBG/Alg	1257:1263	nBG/Alg	1257:1263	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	9	48	from	formation	1239:1247	arg1	Alg					1252:1254	Alg	1252:1254	Alg	1252:1254	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	0	49	theme	multi-functional	26:41	arg1	scaffolds					72:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds	0:80	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.	0:154	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.					
32573600	2	50	from	utility	521:527	arg1	medicine					545:552	regenerative medicine	532:552	regenerative medicine	532:552	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	3	51	theme	Alg	624:626	arg1	peaks					644:648	nBG and Alg amorphous broad peaks	616:648	nBG and Alg amorphous broad peaks	616:648	The presence of the combeite highly crystalline structure of nBG and Alg amorphous broad peaks were confirmed.					
32573600	2	52	theme	membrane	403:410	arg1	scaffolds					412:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	9	53	theme	KB3-1	1208:1212	arg1	cells					1214:1218	KB3-1 cells	1208:1218	KB3-1 cells	1208:1218	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	7	54	from	Hemocompatibility	966:982	arg1	scaffolds					1017:1025	scaffolds	1017:1025	scaffolds	1017:1025	Hemocompatibility and the hemostatic properties on scaffolds were also systematically investigated.					
32573600	2	55	theme	freeze-drying	460:472	arg1	method					474:479	a sol-gel assisted freeze-drying method	441:479	a sol-gel assisted freeze-drying method	441:479	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	9	56	theme	interest	1338:1345	arg1	organoids					1325:1333	organoids	1325:1333	organoids of interest	1325:1345	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	3	57	theme	nBG	616:618	arg1	peaks					644:648	nBG and Alg amorphous broad peaks	616:648	nBG and Alg amorphous broad peaks	616:648	The presence of the combeite highly crystalline structure of nBG and Alg amorphous broad peaks were confirmed.					
32573600	2	58	theme	particulate	349:359	arg1	scaffolds					412:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	10	59	theme	regenerative	1459:1470	arg1	medicine					1472:1479	regenerative medicine	1459:1479	regenerative medicine	1459:1479	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	5	60	dep	7	870:870	arg1	to					867:868	to	867:868	to	867:868	Swelling ability, porosity, and in vitro degradation and biomineralization were analysed for up to 7 days.					
32573600	10	61	theme	formation	1598:1606	arg1	regeneration					1538:1549	the regeneration	1534:1549	the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments	1534:1639	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	3	62	theme	peaks	644:648	arg1	structure					603:611	the combeite highly crystalline structure	571:611	the combeite highly crystalline structure of nBG and Alg amorphous broad peaks	571:648	The presence of the combeite highly crystalline structure of nBG and Alg amorphous broad peaks were confirmed.					
32573600	2	63	theme	glass	343:347	arg1	scaffolds					412:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	10	64	theme	neo-tissue	1587:1596	arg1	formation					1598:1606	neo-tissue formation	1587:1606	neo-tissue formation	1587:1606	Thus, the results indicate that these 3D hydrogel membranes could offer infinite possibilities in the field of regenerative medicine, notably as an extracellular matrix (ECM) supporting the regeneration of bone, intra-vascularization, and neo-tissue formation, such as cartilage and ligaments.					
32573600	9	65	theme	spheroid	1230:1237	arg1	formation					1239:1247	spheroid formation	1230:1247	spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg	1230:1278	Further 3-D co-cultures with HDF cells and KB3-1 cells exhibited spheroid formation on Alg, nBG/Alg and nBG-Zr/Alg with profound dynamism required to establish organoids of interest.					
32573600	2	66	theme	bioactive	333:341	arg1	scaffolds					412:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	4	67	theme	composites	759:768	arg1	interactions					739:750	the chemical interactions	726:750	the chemical interactions of the composites	726:768	Repetitive peaks from acids along with stretching confirmed the chemical interactions of the composites.					
32573600	0	68	theme	bioglass	85:92	arg1	composites					107:116	bioglass and alginate composites	85:116	bioglass and alginate composites	85:116	Bioactive, degradable and multi-functional three-dimensional membranous scaffolds of bioglass and alginate composites for tissue regenerative applications.					
32573600	4	69	theme	Repetitive	666:675	arg1	peaks					677:681	Repetitive peaks	666:681	Repetitive peaks from acids along with stretching	666:714	Repetitive peaks from acids along with stretching confirmed the chemical interactions of the composites.					
32573600	3	70	theme	amorphous	628:636	arg1	peaks					644:648	nBG and Alg amorphous broad peaks	616:648	nBG and Alg amorphous broad peaks	616:648	The presence of the combeite highly crystalline structure of nBG and Alg amorphous broad peaks were confirmed.					
32573600	1	71	theme	worldwide	163:171	arg1	increase					173:180	a worldwide increase	161:180	a worldwide increase in the aged populace and associated geriatric diseases	161:235	With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems.					
32573600	1	72	theme	enormous	250:257	arg1	need					259:262	an enormous need	247:262	an enormous need for the regeneration of degenerated organ systems	247:312	With a worldwide increase in the aged populace and associated geriatric diseases, there is an enormous need for the regeneration of degenerated organ systems.					
32573600	2	73	theme	multifunctional	505:519	arg1	utility					521:527	their multifunctional utility	499:527	their multifunctional utility in regenerative medicine	499:552	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	3	74	theme	broad	638:642	arg1	peaks					644:648	nBG and Alg amorphous broad peaks	616:648	nBG and Alg amorphous broad peaks	616:648	The presence of the combeite highly crystalline structure of nBG and Alg amorphous broad peaks were confirmed.					
32573600	2	75	theme	integrated	367:376	arg1	scaffolds					412:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds	333:420	For this purpose, bioactive glass particulate (nBG) integrated alginate (Alg) composite membrane scaffolds were fabricated by a sol-gel assisted freeze-drying method and validated for their multifunctional utility in regenerative medicine.					
32573600	5	76	theme	Swelling	771:778	arg1	ability					780:786	Swelling ability	771:786	Swelling ability	771:786	Swelling ability, porosity, and in vitro degradation and biomineralization were analysed for up to 7 days.					
34329132	11	0	theme	Pt	1524:1525	arg1	NPs					1527:1529	Pt NPs	1524:1529	Pt NPs	1524:1529	This CNF-PEI aerogel loaded with Pt NPs is recovered easily from wastewater after treatment, so it is reusable and offers high potential as a practical recyclable environmental catalyst.					
34329132	4	1	theme	3-dimensional	556:568	arg1	aerogel					628:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel	556:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs	556:685	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	10	2	theme	NPs	1463:1465	arg1	cycle					1451:1455	5 times reuse cycle	1437:1455	5 times reuse cycle of Pt NPs loaded CNF-PEI aerogel	1437:1488	Finally, 4-NP reduction efficiency does not decrease during 5 times reuse cycle of Pt NPs loaded CNF-PEI aerogel.					
34329132	9	3	theme	fast	1336:1339	arg1	kinetics					1350:1357	fast reaction kinetics	1336:1357	fast reaction kinetics (k = 0.12 min-1)	1336:1374	The 2.74 nm size Pt NPs with even distribution in the aerogel show fast reaction kinetics (k = 0.12 min-1).					
34329132	9	3	theme	fast	1336:1339	arg1	min-1					1369:1373	k = 0.12 min-1	1360:1373	k = 0.12 min-1	1360:1373	The 2.74 nm size Pt NPs with even distribution in the aerogel show fast reaction kinetics (k = 0.12 min-1).					
34329132	2	4	from	effluent	316:323	arg1	4-NP					300:303	4-NP	300:303	4-NP from waste effluent	300:323	Catalytic reduction of 4-NP using metal nanoparticles (NPs) is a highly effective method to treat 4-NP from waste effluent.					
34329132	8	5	theme	conversion	1166:1175	arg1	activity					1177:1184	The 4-NP conversion activity	1157:1184	The 4-NP conversion activity	1157:1184	The 4-NP conversion activity is strongly affected by the size of Pt NPs and effective surface area of aerogels.					
34329132	7	6	theme	high	1109:1112	arg1	activity					1124:1131	high catalytic activity	1109:1131	high catalytic activity for conversion of 4-NP	1109:1154	The composite aerogel shows high catalytic activity for conversion of 4-NP.					
34329132	5	7	theme	adsorption	815:824	arg1	reaction					826:833	adsorption reaction	815:833	adsorption reaction with amine groups of PEI	815:858	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	4	8	theme	porous	641:646	arg1	material					656:663	a porous support material	639:663	a porous support material	639:663	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	1	9	theme	various	182:188	arg1	industries					190:199	various industries	182:199	various industries	182:199	4-Nitrophenol (4-NP) is a hazardous aromatic compound widely used for various industries.					
34329132	6	10	theme	density	923:929	arg1	Control					899:905	Control	899:905	Control of PEI grafting density and the initial concentration of Pt ions	899:970	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	6	11	theme	aerogel	1072:1078	arg1	scaffold					1052:1059	3D scaffold	1049:1059	3D scaffold of CNF-PEI aerogel	1049:1078	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	10	12	theme	CNF-PEI	1474:1480	arg1	aerogel					1482:1488	CNF-PEI aerogel	1474:1488	CNF-PEI aerogel	1474:1488	Finally, 4-NP reduction efficiency does not decrease during 5 times reuse cycle of Pt NPs loaded CNF-PEI aerogel.					
34329132	5	13	theme	cylindrical	784:794	arg1	aerogel					804:810	cylindrical CNF-PEI aerogel	784:810	cylindrical CNF-PEI aerogel	784:810	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	6	14	theme	PEI	910:912	arg1	density					923:929	PEI grafting density	910:929	PEI grafting density	910:929	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	7	15	theme	composite	1085:1093	arg1	aerogel					1095:1101	The composite aerogel	1081:1101	The composite aerogel	1081:1101	The composite aerogel shows high catalytic activity for conversion of 4-NP.					
34329132	8	16	theme	NPs	1225:1227	arg1	size					1214:1217	the size	1210:1217	the size of Pt NPs	1210:1227	The 4-NP conversion activity is strongly affected by the size of Pt NPs and effective surface area of aerogels.					
34329132	8	16	theme	NPs	1225:1227	arg1	area					1251:1254	effective surface area	1233:1254	effective surface area of aerogels	1233:1266	The 4-NP conversion activity is strongly affected by the size of Pt NPs and effective surface area of aerogels.					
34329132	8	17	theme	effective	1233:1241	arg1	area					1251:1254	effective surface area	1233:1254	effective surface area of aerogels	1233:1266	The 4-NP conversion activity is strongly affected by the size of Pt NPs and effective surface area of aerogels.					
34329132	11	18	theme	high	1613:1616	arg1	potential					1618:1626	high potential	1613:1626	high potential as a practical recyclable environmental catalyst	1613:1675	This CNF-PEI aerogel loaded with Pt NPs is recovered easily from wastewater after treatment, so it is reusable and offers high potential as a practical recyclable environmental catalyst.					
34329132	1	19	theme	hazardous	138:146	arg1	4-Nitrophenol					112:124	4-Nitrophenol	112:124	4-Nitrophenol (4-NP)	112:131	4-Nitrophenol (4-NP) is a hazardous aromatic compound widely used for various industries.					
34329132	1	19	theme	hazardous	138:146	arg1	compound					157:164	a hazardous aromatic compound	136:164	a hazardous aromatic compound widely used for various industries	136:199	4-Nitrophenol (4-NP) is a hazardous aromatic compound widely used for various industries.					
34329132	6	20	theme	Pt	964:965	arg1	ions					967:970	Pt ions	964:970	Pt ions	964:970	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	4	21	theme	platinum	669:676	arg1	NPs					683:685	platinum (Pt) NPs	669:685	platinum (Pt) NPs	669:685	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	11	22	theme	environmental	1654:1666	arg1	catalyst					1668:1675	a practical recyclable environmental catalyst	1631:1675	a practical recyclable environmental catalyst	1631:1675	This CNF-PEI aerogel loaded with Pt NPs is recovered easily from wastewater after treatment, so it is reusable and offers high potential as a practical recyclable environmental catalyst.					
34329132	7	23	theme	4-NP	1151:1154	arg1	conversion					1137:1146	conversion	1137:1146	conversion of 4-NP	1137:1154	The composite aerogel shows high catalytic activity for conversion of 4-NP.					
34329132	4	24	from	wastewater	734:743	arg1	4-NP					724:727	4-NP	724:727	4-NP from wastewater	724:743	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	5	25	theme	PEI	856:858	arg1	PEI					856:858	PEI	856:858	PEI	856:858	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	5	25	theme	PEI	856:858	arg1	groups					846:851	amine groups	840:851	amine groups of PEI	840:858	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	6	26	theme	concentration	947:959	arg1	Control					899:905	Control	899:905	Control of PEI grafting density and the initial concentration of Pt ions	899:970	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	8	27	theme	aerogels	1259:1266	arg1	size					1214:1217	the size	1210:1217	the size of Pt NPs	1210:1227	The 4-NP conversion activity is strongly affected by the size of Pt NPs and effective surface area of aerogels.					
34329132	8	27	theme	aerogels	1259:1266	arg1	area					1251:1254	effective surface area	1233:1254	effective surface area of aerogels	1233:1266	The 4-NP conversion activity is strongly affected by the size of Pt NPs and effective surface area of aerogels.					
34329132	11	28	theme	practical	1633:1641	arg1	catalyst					1668:1675	a practical recyclable environmental catalyst	1631:1675	a practical recyclable environmental catalyst	1631:1675	This CNF-PEI aerogel loaded with Pt NPs is recovered easily from wastewater after treatment, so it is reusable and offers high potential as a practical recyclable environmental catalyst.					
34329132	5	29	with	reduction	877:885	arg1	NaBH4					892:896	NaBH4	892:896	NaBH4	892:896	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	6	30	dep	mass	1007:1010	arg1	the					995:997	the	995:997	the	995:997	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	0	31	theme	Modified	0:7	arg1	nanofibril					19:28	Modified cellulose nanofibril	0:28	Modified cellulose nanofibril	0:28	Modified cellulose nanofibril aerogel: Tunable catalyst support for treatment of 4-Nitrophenol from wastewater.					
34329132	3	32	theme	methods	346:352	arg1	methods					346:352	methods	346:352	methods	346:352	Even though lots of methods have investigated to prepare efficient metal NPs composites, the nano and/or micro size of composites makes it hard to recover after wastewater treatment, limiting its practical use.					
34329132	3	32	theme	methods	346:352	arg1	lots					338:341	lots	338:341	lots of methods	338:352	Even though lots of methods have investigated to prepare efficient metal NPs composites, the nano and/or micro size of composites makes it hard to recover after wastewater treatment, limiting its practical use.					
34329132	6	33	theme	loading	999:1005	arg1	mass					1007:1010	loading mass	999:1010	loading mass	999:1010	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	3	34	theme	metal	393:397	arg1	composites					403:412	efficient metal NPs composites	383:412	efficient metal NPs composites	383:412	Even though lots of methods have investigated to prepare efficient metal NPs composites, the nano and/or micro size of composites makes it hard to recover after wastewater treatment, limiting its practical use.					
34329132	10	35	theme	4-NP	1386:1389	arg1	efficiency					1401:1410	4-NP reduction efficiency	1386:1410	4-NP reduction efficiency	1386:1410	Finally, 4-NP reduction efficiency does not decrease during 5 times reuse cycle of Pt NPs loaded CNF-PEI aerogel.					
34329132	9	36	theme	2.74 nm	1273:1279	arg1	NPs					1289:1291	The 2.74 nm size Pt NPs	1269:1291	The 2.74 nm size Pt NPs with even distribution in the aerogel	1269:1329	The 2.74 nm size Pt NPs with even distribution in the aerogel show fast reaction kinetics (k = 0.12 min-1).					
34329132	5	37	with	reaction	826:833	arg1	PEI					856:858	PEI	856:858	PEI	856:858	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	5	37	with	reaction	826:833	arg1	groups					846:851	amine groups	840:851	amine groups of PEI	840:858	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	0	38	theme	catalyst	47:54	arg1	support					56:62	Tunable catalyst support	39:62	Tunable catalyst support for treatment of 4-Nitrophenol from wastewater	39:109	Modified cellulose nanofibril aerogel: Tunable catalyst support for treatment of 4-Nitrophenol from wastewater.					
34329132	9	39	theme	Pt	1286:1287	arg1	NPs					1289:1291	The 2.74 nm size Pt NPs	1269:1291	The 2.74 nm size Pt NPs with even distribution in the aerogel	1269:1329	The 2.74 nm size Pt NPs with even distribution in the aerogel show fast reaction kinetics (k = 0.12 min-1).					
34329132	3	40	dep	nano	419:422	arg1	the					415:417	the	415:417	the	415:417	Even though lots of methods have investigated to prepare efficient metal NPs composites, the nano and/or micro size of composites makes it hard to recover after wastewater treatment, limiting its practical use.					
34329132	2	41	theme	Catalytic	202:210	arg1	method					284:289	a highly effective method	265:289	a highly effective method to treat 4-NP from waste effluent	265:323	Catalytic reduction of 4-NP using metal nanoparticles (NPs) is a highly effective method to treat 4-NP from waste effluent.					
34329132	2	41	theme	Catalytic	202:210	arg1	reduction					212:220	Catalytic reduction	202:220	Catalytic reduction of 4-NP using metal nanoparticles (NPs)	202:260	Catalytic reduction of 4-NP using metal nanoparticles (NPs) is a highly effective method to treat 4-NP from waste effluent.					
34329132	2	42	theme	waste	310:314	arg1	effluent					316:323	waste effluent	310:323	waste effluent	310:323	Catalytic reduction of 4-NP using metal nanoparticles (NPs) is a highly effective method to treat 4-NP from waste effluent.					
34329132	4	43	theme	polyethylene	570:581	arg1	aerogel					628:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel	556:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs	556:685	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	6	44	theme	Pt	1039:1040	arg1	NPs					1042:1044	Pt NPs	1039:1044	Pt NPs	1039:1044	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	4	45	theme	grafted	589:595	arg1	aerogel					628:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel	556:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs	556:685	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	10	46	theme	times	1439:1443	arg1	cycle					1451:1455	5 times reuse cycle	1437:1455	5 times reuse cycle of Pt NPs loaded CNF-PEI aerogel	1437:1488	Finally, 4-NP reduction efficiency does not decrease during 5 times reuse cycle of Pt NPs loaded CNF-PEI aerogel.					
34329132	6	47	theme	distribution	1023:1034	arg1	manipulation					979:990	manipulation	979:990	manipulation of the loading mass, size, and distribution of Pt NPs	979:1044	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	9	48	with	NPs	1289:1291	arg1	distribution					1303:1314	even distribution	1298:1314	even distribution in the aerogel	1298:1329	The 2.74 nm size Pt NPs with even distribution in the aerogel show fast reaction kinetics (k = 0.12 min-1).					
34329132	2	49	theme	effective	274:282	arg1	method					284:289	a highly effective method	265:289	a highly effective method to treat 4-NP from waste effluent	265:323	Catalytic reduction of 4-NP using metal nanoparticles (NPs) is a highly effective method to treat 4-NP from waste effluent.					
34329132	2	49	theme	effective	274:282	arg1	reduction					212:220	Catalytic reduction	202:220	Catalytic reduction of 4-NP using metal nanoparticles (NPs)	202:260	Catalytic reduction of 4-NP using metal nanoparticles (NPs) is a highly effective method to treat 4-NP from waste effluent.					
34329132	10	50	theme	Pt	1460:1461	arg1	NPs					1463:1465	Pt NPs	1460:1465	Pt NPs loaded CNF-PEI aerogel	1460:1488	Finally, 4-NP reduction efficiency does not decrease during 5 times reuse cycle of Pt NPs loaded CNF-PEI aerogel.					
34329132	9	51	theme	reaction	1341:1348	arg1	kinetics					1350:1357	fast reaction kinetics	1336:1357	fast reaction kinetics (k = 0.12 min-1)	1336:1374	The 2.74 nm size Pt NPs with even distribution in the aerogel show fast reaction kinetics (k = 0.12 min-1).					
34329132	9	51	theme	reaction	1341:1348	arg1	min-1					1369:1373	k = 0.12 min-1	1360:1373	k = 0.12 min-1	1360:1373	The 2.74 nm size Pt NPs with even distribution in the aerogel show fast reaction kinetics (k = 0.12 min-1).					
34329132	8	52	theme	4-NP	1161:1164	arg1	activity					1177:1184	The 4-NP conversion activity	1157:1184	The 4-NP conversion activity	1157:1184	The 4-NP conversion activity is strongly affected by the size of Pt NPs and effective surface area of aerogels.					
34329132	3	53	theme	practical	522:530	arg1	use					532:534	its practical use	518:534	its practical use	518:534	Even though lots of methods have investigated to prepare efficient metal NPs composites, the nano and/or micro size of composites makes it hard to recover after wastewater treatment, limiting its practical use.					
34329132	6	54	theme	grafting	914:921	arg1	density					923:929	PEI grafting density	910:929	PEI grafting density	910:929	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	6	55	theme	CNF-PEI	1064:1070	arg1	aerogel					1072:1078	CNF-PEI aerogel	1064:1078	CNF-PEI aerogel	1064:1078	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	4	56	theme	nanofibril	607:616	arg1	aerogel					628:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel	556:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs	556:685	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	2	57	theme	metal	236:240	arg1	NPs					257:259	NPs	257:259	NPs	257:259	Catalytic reduction of 4-NP using metal nanoparticles (NPs) is a highly effective method to treat 4-NP from waste effluent.					
34329132	2	57	theme	metal	236:240	arg1	nanoparticles					242:254	metal nanoparticles	236:254	metal nanoparticles (NPs)	236:260	Catalytic reduction of 4-NP using metal nanoparticles (NPs) is a highly effective method to treat 4-NP from waste effluent.					
34329132	5	58	theme	CNF-PEI	796:802	arg1	aerogel					804:810	cylindrical CNF-PEI aerogel	784:810	cylindrical CNF-PEI aerogel	784:810	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	0	59	from	treatment	68:76	arg1	wastewater					100:109	wastewater	100:109	wastewater	100:109	Modified cellulose nanofibril aerogel: Tunable catalyst support for treatment of 4-Nitrophenol from wastewater.					
34329132	4	60	theme	CNF-PEI	619:625	arg1	aerogel					628:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel	556:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs	556:685	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	2	61	theme	4-NP	225:228	arg1	method					284:289	a highly effective method	265:289	a highly effective method to treat 4-NP from waste effluent	265:323	Catalytic reduction of 4-NP using metal nanoparticles (NPs) is a highly effective method to treat 4-NP from waste effluent.					
34329132	2	61	theme	4-NP	225:228	arg1	reduction					212:220	Catalytic reduction	202:220	Catalytic reduction of 4-NP using metal nanoparticles (NPs)	202:260	Catalytic reduction of 4-NP using metal nanoparticles (NPs) is a highly effective method to treat 4-NP from waste effluent.					
34329132	0	62	from	wastewater	100:109	arg1	4-Nitrophenol					81:93	4-Nitrophenol	81:93	4-Nitrophenol from wastewater	81:109	Modified cellulose nanofibril aerogel: Tunable catalyst support for treatment of 4-Nitrophenol from wastewater.					
34329132	0	62	from	wastewater	100:109	arg1	treatment					68:76	treatment	68:76	treatment of 4-Nitrophenol from wastewater	68:109	Modified cellulose nanofibril aerogel: Tunable catalyst support for treatment of 4-Nitrophenol from wastewater.					
34329132	8	63	theme	Pt	1222:1223	arg1	NPs					1225:1227	Pt NPs	1222:1227	Pt NPs	1222:1227	The 4-NP conversion activity is strongly affected by the size of Pt NPs and effective surface area of aerogels.					
34329132	3	64	theme	composites	445:454	arg1	nano					419:422	nano	419:422	nano	419:422	Even though lots of methods have investigated to prepare efficient metal NPs composites, the nano and/or micro size of composites makes it hard to recover after wastewater treatment, limiting its practical use.					
34329132	5	65	theme	amine	840:844	arg1	PEI					856:858	PEI	856:858	PEI	856:858	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	5	65	theme	amine	840:844	arg1	groups					846:851	amine groups	840:851	amine groups of PEI	840:858	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	6	66	theme	ions	967:970	arg1	concentration					947:959	the initial concentration	935:959	the initial concentration of Pt ions	935:970	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	6	66	theme	ions	967:970	arg1	density					923:929	PEI grafting density	910:929	PEI grafting density	910:929	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	4	67	theme	support	648:654	arg1	material					656:663	a porous support material	639:663	a porous support material	639:663	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	0	68	theme	4-Nitrophenol	81:93	arg1	treatment					68:76	treatment	68:76	treatment of 4-Nitrophenol from wastewater	68:109	Modified cellulose nanofibril aerogel: Tunable catalyst support for treatment of 4-Nitrophenol from wastewater.					
34329132	1	69	theme	aromatic	148:155	arg1	4-Nitrophenol					112:124	4-Nitrophenol	112:124	4-Nitrophenol (4-NP)	112:131	4-Nitrophenol (4-NP) is a hazardous aromatic compound widely used for various industries.					
34329132	1	69	theme	aromatic	148:155	arg1	compound					157:164	a hazardous aromatic compound	136:164	a hazardous aromatic compound widely used for various industries	136:199	4-Nitrophenol (4-NP) is a hazardous aromatic compound widely used for various industries.					
34329132	8	70	theme	surface	1243:1249	arg1	area					1251:1254	effective surface area	1233:1254	effective surface area of aerogels	1233:1266	The 4-NP conversion activity is strongly affected by the size of Pt NPs and effective surface area of aerogels.					
34329132	6	71	theme	initial	939:945	arg1	concentration					947:959	the initial concentration	935:959	the initial concentration of Pt ions	935:970	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	11	72	theme	recyclable	1643:1652	arg1	catalyst					1668:1675	a practical recyclable environmental catalyst	1631:1675	a practical recyclable environmental catalyst	1631:1675	This CNF-PEI aerogel loaded with Pt NPs is recovered easily from wastewater after treatment, so it is reusable and offers high potential as a practical recyclable environmental catalyst.					
34329132	7	73	theme	catalytic	1114:1122	arg1	activity					1124:1131	high catalytic activity	1109:1131	high catalytic activity for conversion of 4-NP	1109:1154	The composite aerogel shows high catalytic activity for conversion of 4-NP.					
34329132	5	74	from	mode	776:779	arg1	aerogel					804:810	cylindrical CNF-PEI aerogel	784:810	cylindrical CNF-PEI aerogel	784:810	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	0	75	theme	cellulose	9:17	arg1	nanofibril					19:28	Modified cellulose nanofibril	0:28	Modified cellulose nanofibril	0:28	Modified cellulose nanofibril aerogel: Tunable catalyst support for treatment of 4-Nitrophenol from wastewater.					
34329132	6	76	theme	size	1013:1016	arg1	manipulation					979:990	manipulation	979:990	manipulation of the loading mass, size, and distribution of Pt NPs	979:1044	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	5	77	theme	in-situ	768:774	arg1	mode					776:779	in-situ mode	768:779	in-situ mode on cylindrical CNF-PEI aerogel	768:810	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	6	78	theme	mass	1007:1010	arg1	manipulation					979:990	manipulation	979:990	manipulation of the loading mass, size, and distribution of Pt NPs	979:1044	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	10	79	theme	reduction	1391:1399	arg1	efficiency					1401:1410	4-NP reduction efficiency	1386:1410	4-NP reduction efficiency	1386:1410	Finally, 4-NP reduction efficiency does not decrease during 5 times reuse cycle of Pt NPs loaded CNF-PEI aerogel.					
34329132	0	80	theme	Tunable	39:45	arg1	support					56:62	Tunable catalyst support	39:62	Tunable catalyst support for treatment of 4-Nitrophenol from wastewater	39:109	Modified cellulose nanofibril aerogel: Tunable catalyst support for treatment of 4-Nitrophenol from wastewater.					
34329132	3	81	theme	NPs	399:401	arg1	composites					403:412	efficient metal NPs composites	383:412	efficient metal NPs composites	383:412	Even though lots of methods have investigated to prepare efficient metal NPs composites, the nano and/or micro size of composites makes it hard to recover after wastewater treatment, limiting its practical use.					
34329132	3	82	theme	micro	431:435	arg1	size					437:440	micro size	431:440	micro size	431:440	Even though lots of methods have investigated to prepare efficient metal NPs composites, the nano and/or micro size of composites makes it hard to recover after wastewater treatment, limiting its practical use.					
34329132	5	83	theme	Pt	750:751	arg1	NPs					753:755	The Pt NPs	746:755	The Pt NPs	746:755	The Pt NPs are formed in-situ mode on cylindrical CNF-PEI aerogel by adsorption reaction with amine groups of PEI and subsequently reduction with NaBH4.					
34329132	9	84	theme	size	1281:1284	arg1	NPs					1289:1291	The 2.74 nm size Pt NPs	1269:1291	The 2.74 nm size Pt NPs with even distribution in the aerogel	1269:1329	The 2.74 nm size Pt NPs with even distribution in the aerogel show fast reaction kinetics (k = 0.12 min-1).					
34329132	9	85	from	distribution	1303:1314	arg1	aerogel					1323:1329	the aerogel	1319:1329	the aerogel	1319:1329	The 2.74 nm size Pt NPs with even distribution in the aerogel show fast reaction kinetics (k = 0.12 min-1).					
34329132	3	86	theme	efficient	383:391	arg1	composites					403:412	efficient metal NPs composites	383:412	efficient metal NPs composites	383:412	Even though lots of methods have investigated to prepare efficient metal NPs composites, the nano and/or micro size of composites makes it hard to recover after wastewater treatment, limiting its practical use.					
34329132	6	87	theme	3D	1049:1050	arg1	scaffold					1052:1059	3D scaffold	1049:1059	3D scaffold of CNF-PEI aerogel	1049:1078	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	4	88	theme	imine	583:587	arg1	aerogel					628:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel	556:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs	556:685	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	9	89	theme	even	1298:1301	arg1	distribution					1303:1314	even distribution	1298:1314	even distribution in the aerogel	1298:1329	The 2.74 nm size Pt NPs with even distribution in the aerogel show fast reaction kinetics (k = 0.12 min-1).					
34329132	6	90	theme	NPs	1042:1044	arg1	size					1013:1016	size	1013:1016	size	1013:1016	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	6	90	theme	NPs	1042:1044	arg1	distribution					1023:1034	distribution	1023:1034	distribution	1023:1034	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	6	90	theme	NPs	1042:1044	arg1	mass					1007:1010	loading mass	999:1010	loading mass	999:1010	Control of PEI grafting density and the initial concentration of Pt ions allows manipulation of the loading mass, size, and distribution of Pt NPs on 3D scaffold of CNF-PEI aerogel.					
34329132	4	91	theme	cellulose	597:605	arg1	aerogel					628:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel	556:634	3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs	556:685	Here, we fabricate 3-dimensional polyethylene imine grafted cellulose nanofibril (CNF-PEI) aerogel as a porous support material for platinum (Pt) NPs to practically and effectively treat 4-NP from wastewater.					
34329132	10	92	theme	reuse	1445:1449	arg1	cycle					1451:1455	5 times reuse cycle	1437:1455	5 times reuse cycle of Pt NPs loaded CNF-PEI aerogel	1437:1488	Finally, 4-NP reduction efficiency does not decrease during 5 times reuse cycle of Pt NPs loaded CNF-PEI aerogel.					
34329132	11	93	theme	CNF-PEI	1496:1502	arg1	aerogel					1504:1510	This CNF-PEI aerogel	1491:1510	This CNF-PEI aerogel loaded with Pt NPs	1491:1529	This CNF-PEI aerogel loaded with Pt NPs is recovered easily from wastewater after treatment, so it is reusable and offers high potential as a practical recyclable environmental catalyst.					
34329132	3	94	theme	wastewater	487:496	arg1	treatment					498:506	wastewater treatment	487:506	wastewater treatment	487:506	Even though lots of methods have investigated to prepare efficient metal NPs composites, the nano and/or micro size of composites makes it hard to recover after wastewater treatment, limiting its practical use.					
32675247	4	0	theme	visible	996:1002	arg1	switches					1010:1017	visible color switches	996:1017	visible color switches	996:1017	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	3	1	theme	assembled	707:715	arg1	structures					726:735	their assembled periodic structures	701:735	their assembled periodic structures	701:735	We found that the HPC could still form cholesteric liquid-crystal photonic structures with the CNTs additive for enhancing their color saturation and PACA polymerization for locating their assembled periodic structures.					
32675247	1	2	theme	mechanical	167:176	arg1	stimuli					178:184	external physical or mechanical stimuli	146:184	external physical or mechanical stimuli	146:184	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	5	3	theme	electrical	1120:1129	arg1	signals					1142:1148	electrical resistance signals	1120:1148	electrical resistance signals	1120:1148	In addition, due to the additive of CNTs, the composite hydrogel could also output these stimuli as electrical resistance signals.					
32675247	4	4	theme	mechanical	873:882	arg1	pressure					884:891	mechanical pressure	873:891	mechanical pressure	873:891	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	4	5	theme	PACA	796:799	arg1	scaffold					801:808	the PACA scaffold	792:808	the PACA scaffold	792:808	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	1	6	theme	artificial	267:276	arg1	devices					258:264	wearable devices	249:264	wearable devices	249:264	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	6	theme	artificial	267:276	arg1	prostheses					278:287	artificial prostheses	267:287	artificial prostheses	267:287	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	4	7	theme	internal	953:960	arg1	nanostructure					962:974	internal nanostructure	953:974	internal nanostructure	953:974	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	3	8	theme	additive	618:625	arg1	CNTs					613:616	the CNTs	609:616	the CNTs additive for enhancing their color saturation and PACA polymerization for locating their assembled periodic structures	609:735	We found that the HPC could still form cholesteric liquid-crystal photonic structures with the CNTs additive for enhancing their color saturation and PACA polymerization for locating their assembled periodic structures.					
32675247	7	9	theme	great	1486:1490	arg1	prospects					1504:1512	great application prospects	1486:1512	great application prospects	1486:1512	This dual-signal sensing provides the ability of visible-user interaction as well as antiinterference, endowing the multifunctional E-skin with great application prospects.					
32675247	7	10	theme	multifunctional	1458:1472	arg1	E-skin					1474:1479	the multifunctional E-skin	1454:1479	the multifunctional E-skin with great application prospects	1454:1512	This dual-signal sensing provides the ability of visible-user interaction as well as antiinterference, endowing the multifunctional E-skin with great application prospects.					
32675247	4	11	theme	HPC	775:777	arg1	elements					779:786	HPC elements	775:786	HPC elements	775:786	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	2	12	theme	liquid-crystal	493:506	arg1	hydrogel					508:515	composited liquid-crystal hydrogel	482:515	composited liquid-crystal hydrogel	482:515	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	7	13	theme	application	1492:1502	arg1	prospects					1504:1512	great application prospects	1486:1512	great application prospects	1486:1512	This dual-signal sensing provides the ability of visible-user interaction as well as antiinterference, endowing the multifunctional E-skin with great application prospects.					
32675247	4	14	theme	temperature	849:859	arg1	variations					861:870	temperature variations	849:870	temperature variations	849:870	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	1	15	theme	Bionic	95:100	arg1	E-skin					119:124	E-skin	119:124	E-skin	119:124	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	15	theme	Bionic	95:100	arg1	skin					113:116	Bionic electronic skin	95:116	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals	95:204	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	7	16	theme	dual-signal	1347:1357	arg1	sensing					1359:1365	This dual-signal sensing	1342:1365	This dual-signal sensing	1342:1365	This dual-signal sensing provides the ability of visible-user interaction as well as antiinterference, endowing the multifunctional E-skin with great application prospects.					
32675247	2	17	theme	composited	482:491	arg1	hydrogel					508:515	composited liquid-crystal hydrogel	482:515	composited liquid-crystal hydrogel	482:515	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	3	18	theme	periodic	717:724	arg1	structures					726:735	their assembled periodic structures	701:735	their assembled periodic structures	701:735	We found that the HPC could still form cholesteric liquid-crystal photonic structures with the CNTs additive for enhancing their color saturation and PACA polymerization for locating their assembled periodic structures.					
32675247	1	19	theme	electronic	102:111	arg1	E-skin					119:124	E-skin	119:124	E-skin	119:124	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	19	theme	electronic	102:111	arg1	skin					113:116	Bionic electronic skin	95:116	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals	95:204	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	20	theme	output	191:196	arg1	signals					198:204	output signals	191:204	output signals	191:204	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	0	21	theme	conductive	12:21	arg1	liquid-crystal					33:46	Bioinspired conductive cellulose liquid-crystal	0:46	Bioinspired conductive cellulose liquid-crystal	0:46	Bioinspired conductive cellulose liquid-crystal hydrogels as multifunctional electrical skins.					
32675247	6	22	theme	electrical	1250:1259	arg1	resistance					1261:1270	electrical resistance	1250:1270	electrical resistance	1250:1270	Thus, the hydrogel E-skins had the ability of quantitatively feeding back external stimuli through electrical resistance as well as visually mapping the stimulating sites by color variation.					
32675247	4	23	theme	composite	745:753	arg1	hydrogel					755:762	the composite hydrogel	741:762	the composite hydrogel containing HPC elements and the PACA scaffold	741:808	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	0	24	theme	Bioinspired	0:10	arg1	liquid-crystal					33:46	Bioinspired conductive cellulose liquid-crystal	0:46	Bioinspired conductive cellulose liquid-crystal	0:46	Bioinspired conductive cellulose liquid-crystal hydrogels as multifunctional electrical skins.					
32675247	1	25	theme	software	290:297	arg1	devices					258:264	wearable devices	249:264	wearable devices	249:264	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	25	theme	software	290:297	arg1	robots					299:304	software robots	290:304	software robots	290:304	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	7	26	theme	antiinterference	1427:1442	arg1	ability					1380:1386	the ability	1376:1386	the ability of visible-user interaction as well as antiinterference	1376:1442	This dual-signal sensing provides the ability of visible-user interaction as well as antiinterference, endowing the multifunctional E-skin with great application prospects.					
32675247	4	27	contain	containing	764:773	arg1	hydrogel					755:762	the composite hydrogel	741:762	the composite hydrogel containing HPC elements and the PACA scaffold	741:808	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	4	27	contain	containing	764:773	arg2	elements					779:786	HPC elements	775:786	HPC elements	775:786	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	4	27	contain	containing	764:773	arg2	scaffold					801:808	the PACA scaffold	792:808	the PACA scaffold	792:808	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	7	28	theme	visible-user	1391:1402	arg1	interaction					1404:1414	visible-user interaction	1391:1414	visible-user interaction as well as antiinterference	1391:1442	This dual-signal sensing provides the ability of visible-user interaction as well as antiinterference, endowing the multifunctional E-skin with great application prospects.					
32675247	2	29	theme	Acrylamide-co-Acrylic	418:438	arg1	Poly					413:416	Poly	413:416	Poly(Acrylamide-co-Acrylic acid) (PACA)	413:451	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	2	29	theme	Acrylamide-co-Acrylic	418:438	arg1	acid					440:443	Acrylamide-co-Acrylic acid	418:443	Acrylamide-co-Acrylic acid	418:443	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	0	30	theme	cellulose	23:31	arg1	liquid-crystal					33:46	Bioinspired conductive cellulose liquid-crystal	0:46	Bioinspired conductive cellulose liquid-crystal	0:46	Bioinspired conductive cellulose liquid-crystal hydrogels as multifunctional electrical skins.					
32675247	2	31	theme	hydroxypropyl	382:394	arg1	HPC					407:409	HPC	407:409	HPC	407:409	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	2	31	theme	hydroxypropyl	382:394	arg1	cellulose					396:404	hydroxypropyl cellulose	382:404	hydroxypropyl cellulose (HPC)	382:410	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	1	32	contain	has	206:208	arg2	devices					258:264	wearable devices	249:264	wearable devices	249:264	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	32	contain	has	206:208	arg2	range					217:221	a wide range	210:221	a wide range of applications including wearable devices, artificial prostheses, software robots, etc	210:309	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	32	contain	has	206:208	arg1	E-skin					119:124	E-skin	119:124	E-skin	119:124	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	32	contain	has	206:208	arg1	skin					113:116	Bionic electronic skin	95:116	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals	95:204	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	33	theme	wide	212:215	arg1	devices					258:264	wearable devices	249:264	wearable devices	249:264	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	33	theme	wide	212:215	arg1	range					217:221	a wide range	210:221	a wide range of applications including wearable devices, artificial prostheses, software robots, etc	210:309	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	2	34	theme	carbon	458:463	arg1	CNTs					476:479	CNTs	476:479	CNTs	476:479	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	2	34	theme	carbon	458:463	arg1	nanotubes					465:473	carbon nanotubes	458:473	carbon nanotubes (CNTs)	458:480	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	3	35	theme	color	647:651	arg1	saturation					653:662	color saturation	647:662	color saturation	647:662	We found that the HPC could still form cholesteric liquid-crystal photonic structures with the CNTs additive for enhancing their color saturation and PACA polymerization for locating their assembled periodic structures.					
32675247	3	36	theme	photonic	584:591	arg1	structures					593:602	cholesteric liquid-crystal photonic structures	557:602	cholesteric liquid-crystal photonic structures	557:602	We found that the HPC could still form cholesteric liquid-crystal photonic structures with the CNTs additive for enhancing their color saturation and PACA polymerization for locating their assembled periodic structures.					
32675247	4	37	theme	color	1004:1008	arg1	switches					1010:1017	visible color switches	996:1017	visible color switches	996:1017	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	6	38	theme	color	1325:1329	arg1	variation					1331:1339	color variation	1325:1339	color variation	1325:1339	Thus, the hydrogel E-skins had the ability of quantitatively feeding back external stimuli through electrical resistance as well as visually mapping the stimulating sites by color variation.					
32675247	5	39	theme	composite	1066:1074	arg1	hydrogel					1076:1083	the composite hydrogel	1062:1083	the composite hydrogel	1062:1083	In addition, due to the additive of CNTs, the composite hydrogel could also output these stimuli as electrical resistance signals.					
32675247	4	40	theme	different	822:830	arg1	variations					861:870	temperature variations	849:870	temperature variations	849:870	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	4	40	theme	different	822:830	arg1	stimuli					832:838	different stimuli	822:838	different stimuli	822:838	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	4	40	theme	different	822:830	arg1	tension					898:904	tension	898:904	tension	898:904	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	4	40	theme	different	822:830	arg1	pressure					884:891	mechanical pressure	873:891	mechanical pressure	873:891	As the composite hydrogel containing HPC elements and the PACA scaffold responds to different stimuli, such as temperature variations, mechanical pressure, and tension, it could correspondingly change its volume or internal nanostructure and report these as visible color switches.					
32675247	0	41	theme	electrical	77:86	arg1	skins					88:92	multifunctional electrical skins	61:92	multifunctional electrical skins	61:92	Bioinspired conductive cellulose liquid-crystal hydrogels as multifunctional electrical skins.					
32675247	7	42	theme	interaction	1404:1414	arg1	ability					1380:1386	the ability	1376:1386	the ability of visible-user interaction as well as antiinterference	1376:1442	This dual-signal sensing provides the ability of visible-user interaction as well as antiinterference, endowing the multifunctional E-skin with great application prospects.					
32675247	3	43	theme	cholesteric	557:567	arg1	structures					593:602	cholesteric liquid-crystal photonic structures	557:602	cholesteric liquid-crystal photonic structures	557:602	We found that the HPC could still form cholesteric liquid-crystal photonic structures with the CNTs additive for enhancing their color saturation and PACA polymerization for locating their assembled periodic structures.					
32675247	2	44	theme	multifunctional	350:364	arg1	E-skin					366:371	a chameleon-inspired multifunctional E-skin	329:371	a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel	329:515	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	0	45	theme	multifunctional	61:75	arg1	skins					88:92	multifunctional electrical skins	61:92	multifunctional electrical skins	61:92	Bioinspired conductive cellulose liquid-crystal hydrogels as multifunctional electrical skins.					
32675247	3	46	theme	PACA	668:671	arg1	polymerization					673:686	PACA polymerization	668:686	PACA polymerization	668:686	We found that the HPC could still form cholesteric liquid-crystal photonic structures with the CNTs additive for enhancing their color saturation and PACA polymerization for locating their assembled periodic structures.					
32675247	3	47	theme	liquid-crystal	569:582	arg1	structures					593:602	cholesteric liquid-crystal photonic structures	557:602	cholesteric liquid-crystal photonic structures	557:602	We found that the HPC could still form cholesteric liquid-crystal photonic structures with the CNTs additive for enhancing their color saturation and PACA polymerization for locating their assembled periodic structures.					
32675247	2	48	theme	chameleon-inspired	331:348	arg1	E-skin					366:371	a chameleon-inspired multifunctional E-skin	329:371	a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel	329:515	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	7	49	with	E-skin	1474:1479	arg1	prospects					1504:1512	great application prospects	1486:1512	great application prospects	1486:1512	This dual-signal sensing provides the ability of visible-user interaction as well as antiinterference, endowing the multifunctional E-skin with great application prospects.					
32675247	6	50	theme	stimulating	1304:1314	arg1	sites					1316:1320	the stimulating sites	1300:1320	the stimulating sites	1300:1320	Thus, the hydrogel E-skins had the ability of quantitatively feeding back external stimuli through electrical resistance as well as visually mapping the stimulating sites by color variation.					
32675247	6	51	theme	external	1225:1232	arg1	stimuli					1234:1240	back external stimuli	1220:1240	back external stimuli	1220:1240	Thus, the hydrogel E-skins had the ability of quantitatively feeding back external stimuli through electrical resistance as well as visually mapping the stimulating sites by color variation.					
32675247	1	52	theme	applications	226:237	arg1	devices					258:264	wearable devices	249:264	wearable devices	249:264	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	52	theme	applications	226:237	arg1	range					217:221	a wide range	210:221	a wide range of applications including wearable devices, artificial prostheses, software robots, etc	210:309	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	2	53	dep	cellulose	396:404	arg1	hydrogel					508:515	composited liquid-crystal hydrogel	482:515	composited liquid-crystal hydrogel	482:515	Here, we present a chameleon-inspired multifunctional E-skin based on hydroxypropyl cellulose (HPC), Poly(Acrylamide-co-Acrylic acid) (PACA), and carbon nanotubes (CNTs) composited liquid-crystal hydrogel.					
32675247	1	54	theme	external	146:153	arg1	stimuli					178:184	external physical or mechanical stimuli	146:184	external physical or mechanical stimuli	146:184	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	5	55	theme	CNTs	1056:1059	arg1	additive					1044:1051	additive	1044:1051	additive	1044:1051	In addition, due to the additive of CNTs, the composite hydrogel could also output these stimuli as electrical resistance signals.					
32675247	5	56	theme	resistance	1131:1140	arg1	signals					1142:1148	electrical resistance signals	1120:1148	electrical resistance signals	1120:1148	In addition, due to the additive of CNTs, the composite hydrogel could also output these stimuli as electrical resistance signals.					
32675247	1	57	theme	physical	155:162	arg1	stimuli					178:184	external physical or mechanical stimuli	146:184	external physical or mechanical stimuli	146:184	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	6	58	theme	hydrogel	1161:1168	arg1	E-skins					1170:1176	the hydrogel E-skins	1157:1176	the hydrogel E-skins	1157:1176	Thus, the hydrogel E-skins had the ability of quantitatively feeding back external stimuli through electrical resistance as well as visually mapping the stimulating sites by color variation.					
32675247	1	59	theme	wearable	249:256	arg1	etc					307:309	etc	307:309	etc	307:309	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	59	theme	wearable	249:256	arg1	devices					258:264	wearable devices	249:264	wearable devices	249:264	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	59	theme	wearable	249:256	arg1	prostheses					278:287	artificial prostheses	267:287	artificial prostheses	267:287	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	1	59	theme	wearable	249:256	arg1	robots					299:304	software robots	290:304	software robots	290:304	Bionic electronic skin (E-skin) that could convert external physical or mechanical stimuli into output signals has a wide range of applications including wearable devices, artificial prostheses, software robots, etc.					
32675247	5	60	from	output	1096:1101	arg1	addition					1023:1030	addition	1023:1030	addition	1023:1030	In addition, due to the additive of CNTs, the composite hydrogel could also output these stimuli as electrical resistance signals.					
32675247	6	61	contain	had	1178:1180	arg1	E-skins					1170:1176	the hydrogel E-skins	1157:1176	the hydrogel E-skins	1157:1176	Thus, the hydrogel E-skins had the ability of quantitatively feeding back external stimuli through electrical resistance as well as visually mapping the stimulating sites by color variation.					
32675247	6	61	contain	had	1178:1180	arg2	ability					1186:1192	the ability	1182:1192	the ability of quantitatively feeding back external stimuli through electrical resistance as well as visually mapping the stimulating sites by color variation	1182:1339	Thus, the hydrogel E-skins had the ability of quantitatively feeding back external stimuli through electrical resistance as well as visually mapping the stimulating sites by color variation.					
33648177	9	0	theme	natural	1287:1293	arg1	agent					1312:1316	a potential natural immunomodulatory agent	1275:1316	a potential natural immunomodulatory agent	1275:1316	Hence, WGFPN might be a potential natural immunomodulatory agent.					
33648177	9	0	theme	natural	1287:1293	arg1	WGFPN					1260:1264	WGFPN	1260:1264	WGFPN	1260:1264	Hence, WGFPN might be a potential natural immunomodulatory agent.					
33648177	6	1	theme	1,6-linked	910:919	arg1	galactan					921:928	the 1,6-linked galactan	906:928	the 1,6-linked galactan	906:928	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	0	2	theme	ginseng	89:95	arg1	flowers					97:103	Panax ginseng flowers	83:103	Panax ginseng flowers	83:103	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	0	3	from	characterization	11:26	arg1	flowers					97:103	Panax ginseng flowers	83:103	Panax ginseng flowers	83:103	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	6	4	theme	Galp	841:844	arg1	Glcp					856:859	terminal Glcp	847:859	terminal Glcp	847:859	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	4	theme	Galp	841:844	arg1	residues					879:886	terminal Manp residues	865:886	terminal Manp residues	865:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	4	theme	Galp	841:844	arg1	amount					805:810	Trace amount	799:810	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues	799:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	4	theme	Galp	841:844	arg1	Glcp					826:829	1,4-linked Glcp	815:829	1,4-linked Glcp	815:829	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	4	theme	Galp	841:844	arg1	Galp					841:844	terminal Galp	832:844	terminal Galp	832:844	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	1	5	theme	Panax	166:170	arg1	flowers					180:186	Panax ginseng flowers	166:186	Panax ginseng flowers	166:186	A neutral polysaccharide fraction (WGFPN) was isolated from Panax ginseng flowers.					
33648177	4	6	dep	branched	614:621	arg1	1 → 4					624:628	1 → 4	624:628	1 → 4	624:628	It possessed a less branched (1 → 4)-β-D-galactan and a significantly branched (1 → 6)-β-D-galactan.					
33648177	0	7	from	flowers	97:103	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	0	7	from	flowers	97:103	arg1	activity					49:56	immunomodulatory activity	32:56	immunomodulatory activity	32:56	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	0	7	from	flowers	97:103	arg1	heterogalactan					63:76	a heterogalactan	61:76	a heterogalactan from Panax ginseng flowers	61:103	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	3	8	used	used	549:552	arg2	Methylation					451:461	Methylation	451:461	Methylation	451:461	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	3	8	used	used	549:552	arg2	spectra					503:509	1D/2D NMR (nuclear magnetic resonance) spectra	464:509	1D/2D NMR (nuclear magnetic resonance) spectra	464:509	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	3	8	used	used	549:552	arg2	hydrolysis					525:534	enzymatic hydrolysis	515:534	enzymatic hydrolysis	515:534	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	6	9	theme	residues	879:886	arg1	Glcp					856:859	terminal Glcp	847:859	terminal Glcp	847:859	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	9	theme	residues	879:886	arg1	residues					879:886	terminal Manp residues	865:886	terminal Manp residues	865:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	9	theme	residues	879:886	arg1	amount					805:810	Trace amount	799:810	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues	799:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	9	theme	residues	879:886	arg1	Glcp					826:829	1,4-linked Glcp	815:829	1,4-linked Glcp	815:829	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	9	theme	residues	879:886	arg1	Galp					841:844	terminal Galp	832:844	terminal Galp	832:844	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	3	10	theme	WGFPN	587:591	arg1	structure					574:582	the structure	570:582	the structure of WGFPN	570:591	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	2	11	theme	refractive	344:353	arg1	index					355:359	refractive index	344:359	refractive index	344:359	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	5	12	theme	α-L-1,5-Araf	754:765	arg1	residues					782:789	α-L-1,5-Araf and t-α-L-Araf residues	754:789	α-L-1,5-Araf and t-α-L-Araf residues at O-3	754:796	The side chains of (1 → 6)-β-D-galactan were branched with α-L-1,5-Araf and t-α-L-Araf residues at O-3.					
33648177	1	13	attach	isolated	152:159	arg2	fraction					131:138	A neutral polysaccharide fraction	106:138	A neutral polysaccharide fraction (WGFPN)	106:146	A neutral polysaccharide fraction (WGFPN) was isolated from Panax ginseng flowers.					
33648177	1	13	attach	isolated	152:159	arg1	flowers					180:186	Panax ginseng flowers	166:186	Panax ginseng flowers	166:186	A neutral polysaccharide fraction (WGFPN) was isolated from Panax ginseng flowers.					
33648177	1	13	attach	isolated	152:159	arg2	WGFPN					141:145	WGFPN	141:145	WGFPN	141:145	A neutral polysaccharide fraction (WGFPN) was isolated from Panax ginseng flowers.					
33648177	5	14	theme	side	699:702	arg1	chains					704:709	The side chains	695:709	The side chains of (1 → 6)-β-D-galactan	695:733	The side chains of (1 → 6)-β-D-galactan were branched with α-L-1,5-Araf and t-α-L-Araf residues at O-3.					
33648177	8	15	theme	mice	1239:1242	arg1	immunity					1179:1186	the immunity	1175:1186	the immunity of cyclophosphamide (CTX)-induced immunosuppressed mice	1175:1242	Moreover, WGFPN could enhance the immunity of cyclophosphamide (CTX)-induced immunosuppressed mice in vivo.					
33648177	6	16	link	1,4-linked	815:824	arg1	Glcp					826:829	1,4-linked Glcp	815:829	1,4-linked Glcp	815:829	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	17	link	1,4-linked	945:954	arg1	galactan					956:963	1,4-linked galactan	945:963	1,4-linked galactan	945:963	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	2	18	theme	scattering	320:329	arg1	detector					331:338	multi-angle laser light scattering detector	296:338	multi-angle laser light scattering detector	296:338	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	6	19	attach	attached	894:901	arg2	Galp					841:844	terminal Galp	832:844	terminal Galp	832:844	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	19	attach	attached	894:901	arg2	residues					879:886	terminal Manp residues	865:886	terminal Manp residues	865:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	19	attach	attached	894:901	arg2	amount					805:810	Trace amount	799:810	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues	799:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	19	attach	attached	894:901	arg2	Glcp					826:829	1,4-linked Glcp	815:829	1,4-linked Glcp	815:829	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	19	attach	attached	894:901	arg1	galactan					921:928	the 1,6-linked galactan	906:928	the 1,6-linked galactan	906:928	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	19	attach	attached	894:901	arg2	Glcp					856:859	terminal Glcp	847:859	terminal Glcp	847:859	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	2	20	theme	11.0 kDa	441:448	arg1	weight					431:436	a molecular weight	419:436	a molecular weight of 11.0 kDa	419:448	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	5	21	theme	t-α-L-Araf	771:780	arg1	residues					782:789	α-L-1,5-Araf and t-α-L-Araf residues	754:789	α-L-1,5-Araf and t-α-L-Araf residues at O-3	754:796	The side chains of (1 → 6)-β-D-galactan were branched with α-L-1,5-Araf and t-α-L-Araf residues at O-3.					
33648177	3	22	dep	Methylation	451:461	arg1	methods					536:542	methods	536:542	methods	536:542	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	6	23	theme	1,4-linked	815:824	arg1	Glcp					826:829	1,4-linked Glcp	815:829	1,4-linked Glcp	815:829	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	2	24	theme	light	314:318	arg1	scattering					320:329	multi-angle laser light scattering	296:329	multi-angle laser light scattering detector	296:338	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	3	25	theme	magnetic	483:490	arg1	resonance					492:500	nuclear magnetic resonance	475:500	1D/2D NMR (nuclear magnetic resonance) spectra	464:509	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	0	26	from	activity	49:56	arg1	flowers					97:103	Panax ginseng flowers	83:103	Panax ginseng flowers	83:103	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	2	27	dep	detector	331:338	arg1	detector					361:368	detector	361:368	detector	361:368	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	2	28	dep	showed	380:385	arg1	heterogalactan					399:412	a heterogalactan	397:412	a heterogalactan with a molecular weight of 11.0 kDa	397:448	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	3	29	theme	NMR	470:472	arg1	spectra					503:509	1D/2D NMR (nuclear magnetic resonance) spectra	464:509	1D/2D NMR (nuclear magnetic resonance) spectra	464:509	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	0	30	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	6	31	theme	terminal	865:872	arg1	residues					879:886	terminal Manp residues	865:886	terminal Manp residues	865:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	5	32	from	O-3	794:796	arg1	residues					782:789	α-L-1,5-Araf and t-α-L-Araf residues	754:789	α-L-1,5-Araf and t-α-L-Araf residues at O-3	754:796	The side chains of (1 → 6)-β-D-galactan were branched with α-L-1,5-Araf and t-α-L-Araf residues at O-3.					
33648177	2	33	theme	molecular	421:429	arg1	weight					431:436	a molecular weight	419:436	a molecular weight of 11.0 kDa	419:448	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	0	34	theme	immunomodulatory	32:47	arg1	activity					49:56	immunomodulatory activity	32:56	immunomodulatory activity	32:56	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	6	35	theme	Trace	799:803	arg1	Glcp					856:859	terminal Glcp	847:859	terminal Glcp	847:859	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	35	theme	Trace	799:803	arg1	residues					879:886	terminal Manp residues	865:886	terminal Manp residues	865:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	35	theme	Trace	799:803	arg1	amount					805:810	Trace amount	799:810	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues	799:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	35	theme	Trace	799:803	arg1	Glcp					826:829	1,4-linked Glcp	815:829	1,4-linked Glcp	815:829	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	35	theme	Trace	799:803	arg1	Galp					841:844	terminal Galp	832:844	terminal Galp	832:844	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	1	36	theme	neutral	108:114	arg1	WGFPN					141:145	WGFPN	141:145	WGFPN	141:145	A neutral polysaccharide fraction (WGFPN) was isolated from Panax ginseng flowers.					
33648177	1	36	theme	neutral	108:114	arg1	fraction					131:138	A neutral polysaccharide fraction	106:138	A neutral polysaccharide fraction (WGFPN)	106:146	A neutral polysaccharide fraction (WGFPN) was isolated from Panax ginseng flowers.					
33648177	6	37	theme	side	980:983	arg1	chains					985:990	side chains	980:990	side chains	980:990	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	5	38	theme	-β-D-galactan	721:733	arg1	chains					704:709	The side chains	695:709	The side chains of (1 → 6)-β-D-galactan	695:733	The side chains of (1 → 6)-β-D-galactan were branched with α-L-1,5-Araf and t-α-L-Araf residues at O-3.					
33648177	2	39	theme	Monosaccharide	189:202	arg1	composition					204:214	Monosaccharide composition	189:214	Monosaccharide composition	189:214	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	1	40	theme	polysaccharide	116:129	arg1	WGFPN					141:145	WGFPN	141:145	WGFPN	141:145	A neutral polysaccharide fraction (WGFPN) was isolated from Panax ginseng flowers.					
33648177	1	40	theme	polysaccharide	116:129	arg1	fraction					131:138	A neutral polysaccharide fraction	106:138	A neutral polysaccharide fraction (WGFPN)	106:146	A neutral polysaccharide fraction (WGFPN) was isolated from Panax ginseng flowers.					
33648177	4	41	theme	branched	614:621	arg1	-β-D-galactan					630:642	a less branched (1 → 4)-β-D-galactan	607:642	a less branched (1 → 4)-β-D-galactan	607:642	It possessed a less branched (1 → 4)-β-D-galactan and a significantly branched (1 → 6)-β-D-galactan.					
33648177	4	42	theme	branched	664:671	arg1	-β-D-galactan					680:692	a significantly branched (1 → 6)-β-D-galactan	648:692	a significantly branched (1 → 6)-β-D-galactan	648:692	It possessed a less branched (1 → 4)-β-D-galactan and a significantly branched (1 → 6)-β-D-galactan.					
33648177	8	43	theme	immunosuppressed	1222:1237	arg1	mice					1239:1242	cyclophosphamide (CTX)-induced immunosuppressed mice	1191:1242	cyclophosphamide (CTX)-induced immunosuppressed mice	1191:1242	Moreover, WGFPN could enhance the immunity of cyclophosphamide (CTX)-induced immunosuppressed mice in vivo.					
33648177	6	44	link	1,6-linked	910:919	arg1	galactan					921:928	the 1,6-linked galactan	906:928	the 1,6-linked galactan	906:928	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	3	45	theme	resonance	492:500	arg1	spectra					503:509	1D/2D NMR (nuclear magnetic resonance) spectra	464:509	1D/2D NMR (nuclear magnetic resonance) spectra	464:509	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	1	46	theme	ginseng	172:178	arg1	flowers					180:186	Panax ginseng flowers	166:186	Panax ginseng flowers	166:186	A neutral polysaccharide fraction (WGFPN) was isolated from Panax ginseng flowers.					
33648177	3	47	theme	1D/2D	464:468	arg1	spectra					503:509	1D/2D NMR (nuclear magnetic resonance) spectra	464:509	1D/2D NMR (nuclear magnetic resonance) spectra	464:509	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	6	48	theme	Manp	874:877	arg1	residues					879:886	terminal Manp residues	865:886	terminal Manp residues	865:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	2	49	theme	exclusion	258:266	arg1	chromatography					268:281	high-performance size exclusion chromatography	236:281	high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector	236:368	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	0	50	theme	heterogalactan	63:76	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	0	50	theme	heterogalactan	63:76	arg1	activity					49:56	immunomodulatory activity	32:56	immunomodulatory activity	32:56	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	8	51	theme	-induced	1213:1220	arg1	mice					1239:1242	cyclophosphamide (CTX)-induced immunosuppressed mice	1191:1242	cyclophosphamide (CTX)-induced immunosuppressed mice	1191:1242	Moreover, WGFPN could enhance the immunity of cyclophosphamide (CTX)-induced immunosuppressed mice in vivo.					
33648177	3	52	theme	nuclear	475:481	arg1	resonance					492:500	nuclear magnetic resonance	475:500	1D/2D NMR (nuclear magnetic resonance) spectra	464:509	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	2	53	theme	size	253:256	arg1	chromatography					268:281	high-performance size exclusion chromatography	236:281	high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector	236:368	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	7	54	theme	macrophage	1054:1063	arg1	phagocytosis					1065:1076	macrophage phagocytosis	1054:1076	macrophage phagocytosis	1054:1076	WGFPN could activate RAW264.7 macrophages through increasing macrophage phagocytosis, releasing NO and secreting TNF-α, IL-6, IFN-γ and IL-1β in vitro.					
33648177	6	55	theme	Glcp	856:859	arg1	Glcp					856:859	terminal Glcp	847:859	terminal Glcp	847:859	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	55	theme	Glcp	856:859	arg1	residues					879:886	terminal Manp residues	865:886	terminal Manp residues	865:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	55	theme	Glcp	856:859	arg1	amount					805:810	Trace amount	799:810	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues	799:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	55	theme	Glcp	856:859	arg1	Glcp					826:829	1,4-linked Glcp	815:829	1,4-linked Glcp	815:829	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	55	theme	Glcp	856:859	arg1	Galp					841:844	terminal Galp	832:844	terminal Galp	832:844	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	2	56	with	heterogalactan	399:412	arg1	weight					431:436	a molecular weight	419:436	a molecular weight of 11.0 kDa	419:448	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	2	57	theme	high-performance	236:251	arg1	chromatography					268:281	high-performance size exclusion chromatography	236:281	high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector	236:368	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	4	58	contain	possessed	597:605	arg2	-β-D-galactan					680:692	a significantly branched (1 → 6)-β-D-galactan	648:692	a significantly branched (1 → 6)-β-D-galactan	648:692	It possessed a less branched (1 → 4)-β-D-galactan and a significantly branched (1 → 6)-β-D-galactan.					
33648177	4	58	contain	possessed	597:605	arg1	It					594:595	It	594:595	It	594:595	It possessed a less branched (1 → 4)-β-D-galactan and a significantly branched (1 → 6)-β-D-galactan.					
33648177	4	58	contain	possessed	597:605	arg2	-β-D-galactan					630:642	a less branched (1 → 4)-β-D-galactan	607:642	a less branched (1 → 4)-β-D-galactan	607:642	It possessed a less branched (1 → 4)-β-D-galactan and a significantly branched (1 → 6)-β-D-galactan.					
33648177	6	59	theme	1,4-linked	945:954	arg1	galactan					956:963	1,4-linked galactan	945:963	1,4-linked galactan	945:963	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	2	60	theme	HPSEC-MALLS-RI	220:233	arg1	analyses					371:378	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses	189:378	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses	189:378	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	2	61	theme	laser	308:312	arg1	scattering					320:329	multi-angle laser light scattering	296:329	multi-angle laser light scattering detector	296:338	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	6	62	theme	terminal	832:839	arg1	Galp					841:844	terminal Galp	832:844	terminal Galp	832:844	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	3	63	theme	enzymatic	515:523	arg1	hydrolysis					525:534	enzymatic hydrolysis	515:534	enzymatic hydrolysis	515:534	Methylation, 1D/2D NMR (nuclear magnetic resonance) spectra and enzymatic hydrolysis methods were used to characterize the structure of WGFPN.					
33648177	5	64	theme	1 → 6	715:719	arg1	-β-D-galactan					721:733	(1 → 6)-β-D-galactan	714:733	(1 → 6)-β-D-galactan	714:733	The side chains of (1 → 6)-β-D-galactan were branched with α-L-1,5-Araf and t-α-L-Araf residues at O-3.					
33648177	6	65	theme	terminal	847:854	arg1	Glcp					856:859	terminal Glcp	847:859	terminal Glcp	847:859	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	2	66	theme	multi-angle	296:306	arg1	scattering					320:329	multi-angle laser light scattering	296:329	multi-angle laser light scattering detector	296:338	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	6	67	theme	Glcp	826:829	arg1	Glcp					856:859	terminal Glcp	847:859	terminal Glcp	847:859	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	67	theme	Glcp	826:829	arg1	residues					879:886	terminal Manp residues	865:886	terminal Manp residues	865:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	67	theme	Glcp	826:829	arg1	amount					805:810	Trace amount	799:810	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues	799:886	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	67	theme	Glcp	826:829	arg1	Glcp					826:829	1,4-linked Glcp	815:829	1,4-linked Glcp	815:829	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	6	67	theme	Glcp	826:829	arg1	Galp					841:844	terminal Galp	832:844	terminal Galp	832:844	Trace amount of 1,4-linked Glcp, terminal Galp, terminal Glcp and terminal Manp residues might attached to the 1,6-linked galactan through O-3 or 1,4-linked galactan through O-6 as side chains.					
33648177	2	68	dep	composition	204:214	arg1	chromatography					268:281	high-performance size exclusion chromatography	236:281	high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector	236:368	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	0	69	theme	Panax	83:87	arg1	flowers					97:103	Panax ginseng flowers	83:103	Panax ginseng flowers	83:103	Structural characterization and immunomodulatory activity of a heterogalactan from Panax ginseng flowers.					
33648177	2	70	theme	composition	204:214	arg1	analyses					371:378	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses	189:378	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses	189:378	Monosaccharide composition and HPSEC-MALLS-RI (high-performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector) analyses showed WGFPN was a heterogalactan with a molecular weight of 11.0 kDa.					
33648177	7	71	theme	RAW264.7	1014:1021	arg1	macrophages					1023:1033	RAW264.7 macrophages	1014:1033	RAW264.7 macrophages	1014:1033	WGFPN could activate RAW264.7 macrophages through increasing macrophage phagocytosis, releasing NO and secreting TNF-α, IL-6, IFN-γ and IL-1β in vitro.					
33648177	9	72	theme	immunomodulatory	1295:1310	arg1	agent					1312:1316	a potential natural immunomodulatory agent	1275:1316	a potential natural immunomodulatory agent	1275:1316	Hence, WGFPN might be a potential natural immunomodulatory agent.					
33648177	9	72	theme	immunomodulatory	1295:1310	arg1	WGFPN					1260:1264	WGFPN	1260:1264	WGFPN	1260:1264	Hence, WGFPN might be a potential natural immunomodulatory agent.					
33648177	9	73	theme	potential	1277:1285	arg1	agent					1312:1316	a potential natural immunomodulatory agent	1275:1316	a potential natural immunomodulatory agent	1275:1316	Hence, WGFPN might be a potential natural immunomodulatory agent.					
33648177	9	73	theme	potential	1277:1285	arg1	WGFPN					1260:1264	WGFPN	1260:1264	WGFPN	1260:1264	Hence, WGFPN might be a potential natural immunomodulatory agent.					
33648177	4	74	dep	-β-D-galactan	680:692	arg1	1 → 6					674:678	1 → 6	674:678	1 → 6	674:678	It possessed a less branched (1 → 4)-β-D-galactan and a significantly branched (1 → 6)-β-D-galactan.					
31830158	0	0	theme	jujuba	78:83	arg1	jujube					61:66	jujube	61:66	jujube (Ziziphus jujuba Mill.)	61:90	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)					
31830158	0	0	theme	jujuba	78:83	arg1	Mill					85:88	Ziziphus jujuba Mill.	69:89	Ziziphus jujuba Mill.	69:89	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)					
31830158	9	1	theme	ATP-binding	1188:1198	arg1	transporters					1215:1226	ATP-binding cassette (ABC) transporters	1188:1226	ATP-binding cassette (ABC) transporters	1188:1226	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	6	2	from	influence	765:773	arg1	structure					798:806	the gut flora structure	784:806	the gut flora structure	784:806	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	0	3	theme	Ziziphus	69:76	arg1	jujube					61:66	jujube	61:66	jujube (Ziziphus jujuba Mill.)	61:90	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)					
31830158	0	3	theme	Ziziphus	69:76	arg1	Mill					85:88	Ziziphus jujuba Mill.	69:89	Ziziphus jujuba Mill.	69:89	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)					
31830158	11	4	theme	promising	1704:1712	arg1	candidates					1724:1733	promising prebiotic candidates	1704:1733	promising prebiotic candidates for the prevention and treatment of colorectal cancer	1704:1787	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	6	5	theme	colitis	851:857	arg1	model					872:876	an AOM/DSS-induced colitis cancer mouse model	832:876	an AOM/DSS-induced colitis cancer mouse model	832:876	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	4	6	theme	functional	416:425	arg1	composition					427:437	the predominant functional composition	400:437	the predominant functional composition found in jujube fruit	400:459	Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models.					
31830158	4	6	theme	functional	416:425	arg1	Polysaccharides					383:397	Polysaccharides	383:397	Polysaccharides	383:397	Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models.					
31830158	9	7	theme	transporters	1215:1226	arg1	pathways					1156:1163	KEGG pathways	1151:1163	KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system	1151:1251	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	11	8	theme	cancer	1782:1787	arg1	prevention					1743:1752	prevention	1743:1752	prevention	1743:1752	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	11	8	theme	cancer	1782:1787	arg1	treatment					1758:1766	treatment	1758:1766	treatment	1758:1766	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	3	9	theme	intestinal	360:369	arg1	microbiota					371:380	the intestinal microbiota	356:380	the intestinal microbiota	356:380	The nondigestible plant polysaccharides have always been fermented by the intestinal microbiota.					
31830158	11	10	theme	prebiotic	1714:1722	arg1	candidates					1724:1733	promising prebiotic candidates	1704:1733	promising prebiotic candidates for the prevention and treatment of colorectal cancer	1704:1787	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	10	11	theme	host	1507:1510	arg1	health					1512:1517	host health	1507:1517	host health	1507:1517	These results suggested that JP showed prebiotic-like activities by positively modulating intestinal microbiota and affecting certain metabolic pathways contributing to host health.					
31830158	4	12	theme	predominant	404:414	arg1	composition					427:437	the predominant functional composition	400:437	the predominant functional composition found in jujube fruit	400:459	Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models.					
31830158	4	12	theme	predominant	404:414	arg1	Polysaccharides					383:397	Polysaccharides	383:397	Polysaccharides	383:397	Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models.					
31830158	6	13	theme	study	652:656	arg1	aim					640:642	The aim	636:642	The aim of this study	636:656	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	9	14	theme	metabolic	1168:1176	arg1	pathways					1178:1185	metabolic pathways	1168:1185	metabolic pathways	1168:1185	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	2	15	theme	Accumulating	144:155	arg1	evidence					157:164	Accumulating evidence	144:164	Accumulating evidence	144:164	Accumulating evidence has reported that the gut microbiota could play important roles in the occurrence and progression of colorectal cancer.					
31830158	9	16	theme	ABC	1210:1212	arg1	transporters					1215:1226	ATP-binding cassette (ABC) transporters	1188:1226	ATP-binding cassette (ABC) transporters	1188:1226	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	2	17	theme	cancer	278:283	arg1	occurrence					237:246	occurrence	237:246	occurrence	237:246	Accumulating evidence has reported that the gut microbiota could play important roles in the occurrence and progression of colorectal cancer.					
31830158	2	17	theme	cancer	278:283	arg1	progression					252:262	progression	252:262	progression	252:262	Accumulating evidence has reported that the gut microbiota could play important roles in the occurrence and progression of colorectal cancer.					
31830158	7	18	theme	control	929:935	arg1	group					937:941	control group	929:941	control group	929:941	Contrasted with control group, the addition of JP could ward off colon cancer by ameliorating colitis cancer-induced gut dysbiosis.					
31830158	8	19	theme	significant	1070:1080	arg1	decrease					1082:1089	a significant decrease	1068:1089	a significant decrease in Firmicutes/Bacteroidetes post JP treatment	1068:1135	In addition, there was a significant decrease in Firmicutes/Bacteroidetes post JP treatment.					
31830158	10	20	theme	intestinal	1428:1437	arg1	microbiota					1439:1448	intestinal microbiota	1428:1448	intestinal microbiota	1428:1448	These results suggested that JP showed prebiotic-like activities by positively modulating intestinal microbiota and affecting certain metabolic pathways contributing to host health.					
31830158	2	21	theme	colorectal	267:276	arg1	cancer					278:283	colorectal cancer	267:283	colorectal cancer	267:283	Accumulating evidence has reported that the gut microbiota could play important roles in the occurrence and progression of colorectal cancer.					
31830158	6	22	theme	intestinal	734:743	arg1	microbiota					745:754	intestinal microbiota	734:754	intestinal microbiota	734:754	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	2	23	dep	occurrence	237:246	arg1	the					233:235	the	233:235	the	233:235	Accumulating evidence has reported that the gut microbiota could play important roles in the occurrence and progression of colorectal cancer.					
31830158	4	24	theme	animal	506:511	arg1	models					513:518	animal models	506:518	animal models	506:518	Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models.					
31830158	7	25	theme	JP	960:961	arg1	addition					948:955	the addition	944:955	the addition of JP	944:961	Contrasted with control group, the addition of JP could ward off colon cancer by ameliorating colitis cancer-induced gut dysbiosis.					
31830158	7	26	theme	cancer-induced	1015:1028	arg1	dysbiosis					1034:1042	colitis cancer-induced gut dysbiosis	1007:1042	colitis cancer-induced gut dysbiosis	1007:1042	Contrasted with control group, the addition of JP could ward off colon cancer by ameliorating colitis cancer-induced gut dysbiosis.					
31830158	4	27	from	carcinogenesis	488:501	arg1	models					513:518	animal models	506:518	animal models	506:518	Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models.					
31830158	10	28	theme	metabolic	1472:1480	arg1	pathways					1482:1489	certain metabolic pathways	1464:1489	certain metabolic pathways contributing to host health	1464:1517	These results suggested that JP showed prebiotic-like activities by positively modulating intestinal microbiota and affecting certain metabolic pathways contributing to host health.					
31830158	0	29	theme	Metagenomic	0:10	arg1	analysis					12:19	Metagenomic analysis	0:19	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)	0:90	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)					
31830158	11	30	theme	gut	1607:1609	arg1	profile					1622:1628	the gut microbiota profile	1603:1628	the gut microbiota profile altered by AOM/DSS	1603:1647	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	9	31	theme	JP	1308:1309	arg1	intervention					1311:1322	JP intervention	1308:1322	JP intervention for 13 weeks	1308:1335	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	6	32	theme	flora	792:796	arg1	structure					798:806	the gut flora structure	784:806	the gut flora structure	784:806	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	6	33	theme	mouse	866:870	arg1	model					872:876	an AOM/DSS-induced colitis cancer mouse model	832:876	an AOM/DSS-induced colitis cancer mouse model	832:876	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	0	34	theme	gut	24:26	arg1	effects					50:56	gut microbiota modulatory effects	24:56	gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)	24:90	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)					
31830158	9	35	theme	two-component	1232:1244	arg1	system					1246:1251	two-component system	1232:1251	two-component system	1232:1251	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	6	36	theme	gut	788:790	arg1	flora					792:796	the gut flora	784:796	the gut flora structure	784:806	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	7	37	theme	colon	978:982	arg1	cancer					984:989	colon cancer	978:989	colon cancer	978:989	Contrasted with control group, the addition of JP could ward off colon cancer by ameliorating colitis cancer-induced gut dysbiosis.					
31830158	10	38	theme	certain	1464:1470	arg1	pathways					1482:1489	certain metabolic pathways	1464:1489	certain metabolic pathways contributing to host health	1464:1517	These results suggested that JP showed prebiotic-like activities by positively modulating intestinal microbiota and affecting certain metabolic pathways contributing to host health.					
31830158	4	39	located	found	439:443	arg1	fruit					455:459	jujube fruit	448:459	jujube fruit	448:459	Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models.					
31830158	4	39	located	found	439:443	arg2	composition					427:437	the predominant functional composition	400:437	the predominant functional composition found in jujube fruit	400:459	Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models.					
31830158	4	39	located	found	439:443	arg2	Polysaccharides					383:397	Polysaccharides	383:397	Polysaccharides	383:397	Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models.					
31830158	7	40	theme	gut	1030:1032	arg1	dysbiosis					1034:1042	colitis cancer-induced gut dysbiosis	1007:1042	colitis cancer-induced gut dysbiosis	1007:1042	Contrasted with control group, the addition of JP could ward off colon cancer by ameliorating colitis cancer-induced gut dysbiosis.					
31830158	6	41	theme	high-throughput	885:899	arg1	sequencing					901:910	high-throughput sequencing	885:910	high-throughput sequencing	885:910	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	6	42	theme	polysaccharides	710:724	arg1	effects					692:698	the modulatory effects	677:698	the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota	677:754	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	11	43	theme	jujube	1678:1683	arg1	polysaccharides					1685:1699	jujube polysaccharides	1678:1699	jujube polysaccharides	1678:1699	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	0	44	theme	modulatory	39:48	arg1	effects					50:56	gut microbiota modulatory effects	24:56	gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)	24:90	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)					
31830158	11	45	theme	polysaccharides	1685:1699	arg1	potential					1665:1673	the potential	1661:1673	the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer	1661:1787	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	6	46	theme	jujube	703:708	arg1	JP					727:728	JP	727:728	JP	727:728	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	6	46	theme	jujube	703:708	arg1	polysaccharides					710:724	jujube polysaccharides	703:724	jujube polysaccharides (JP)	703:729	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	0	47	theme	microbiota	28:37	arg1	effects					50:56	gut microbiota modulatory effects	24:56	gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)	24:90	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)					
31830158	8	48	theme	JP	1124:1125	arg1	treatment					1127:1135	JP treatment	1124:1135	JP treatment	1124:1135	In addition, there was a significant decrease in Firmicutes/Bacteroidetes post JP treatment.					
31830158	10	49	theme	prebiotic-like	1377:1390	arg1	activities					1392:1401	prebiotic-like activities	1377:1401	prebiotic-like activities	1377:1401	These results suggested that JP showed prebiotic-like activities by positively modulating intestinal microbiota and affecting certain metabolic pathways contributing to host health.					
31830158	9	50	theme	pathways	1178:1185	arg1	pathways					1156:1163	KEGG pathways	1151:1163	KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system	1151:1251	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	8	51	from	decrease	1082:1089	arg1	Firmicutes/Bacteroidetes					1094:1117	Firmicutes/Bacteroidetes	1094:1117	Firmicutes/Bacteroidetes post JP treatment	1094:1135	In addition, there was a significant decrease in Firmicutes/Bacteroidetes post JP treatment.					
31830158	3	52	theme	plant	304:308	arg1	polysaccharides					310:324	The nondigestible plant polysaccharides	286:324	The nondigestible plant polysaccharides	286:324	The nondigestible plant polysaccharides have always been fermented by the intestinal microbiota.					
31830158	11	53	theme	colorectal	1771:1780	arg1	cancer					1782:1787	colorectal cancer	1771:1787	colorectal cancer	1771:1787	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	2	54	theme	important	214:222	arg1	roles					224:228	important roles	214:228	important roles	214:228	Accumulating evidence has reported that the gut microbiota could play important roles in the occurrence and progression of colorectal cancer.					
31830158	9	55	theme	KEGG	1151:1154	arg1	pathways					1156:1163	KEGG pathways	1151:1163	KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system	1151:1251	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	9	56	theme	cassette	1200:1207	arg1	transporters					1215:1226	ATP-binding cassette (ABC) transporters	1188:1226	ATP-binding cassette (ABC) transporters	1188:1226	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	0	57	theme	effects	50:56	arg1	analysis					12:19	Metagenomic analysis	0:19	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)	0:90	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)					
31830158	9	58	theme	expressed	1286:1294	arg1	genes					1296:1300	the most differentially expressed genes	1262:1300	the most differentially expressed genes	1262:1300	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	4	59	theme	jujube	448:453	arg1	fruit					455:459	jujube fruit	448:459	jujube fruit	448:459	Polysaccharides, the predominant functional composition found in jujube fruit, has been shown to inhibit carcinogenesis in animal models.					
31830158	6	60	from	effects	692:698	arg1	microbiota					745:754	intestinal microbiota	734:754	intestinal microbiota	734:754	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	6	61	theme	JP	778:779	arg1	influence					765:773	the influence	761:773	the influence of JP on the gut flora structure	761:806	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	7	62	dep	ward	969:972	arg1	off					974:976	off	974:976	off	974:976	Contrasted with control group, the addition of JP could ward off colon cancer by ameliorating colitis cancer-induced gut dysbiosis.					
31830158	9	63	theme	system	1246:1251	arg1	pathways					1156:1163	KEGG pathways	1151:1163	KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system	1151:1251	What's more, KEGG pathways of metabolic pathways, ATP-binding cassette (ABC) transporters and two-component system enriched the most differentially expressed genes after JP intervention for 13 weeks.					
31830158	6	64	theme	cancer	859:864	arg1	model					872:876	an AOM/DSS-induced colitis cancer mouse model	832:876	an AOM/DSS-induced colitis cancer mouse model	832:876	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	11	65	theme	microbiota	1611:1620	arg1	profile					1622:1628	the gut microbiota profile	1603:1628	the gut microbiota profile altered by AOM/DSS	1603:1647	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	1	66	theme	colorectal	113:122	arg1	model					137:141	a colorectal cancer mouse model	111:141	a colorectal cancer mouse model	111:141	polysaccharides in a colorectal cancer mouse model.					
31830158	1	67	from	polysaccharides	92:106	arg1	model					137:141	a colorectal cancer mouse model	111:141	a colorectal cancer mouse model	111:141	polysaccharides in a colorectal cancer mouse model.					
31830158	3	68	theme	nondigestible	290:302	arg1	polysaccharides					310:324	The nondigestible plant polysaccharides	286:324	The nondigestible plant polysaccharides	286:324	The nondigestible plant polysaccharides have always been fermented by the intestinal microbiota.					
31830158	2	69	theme	gut	188:190	arg1	microbiota					192:201	the gut microbiota	184:201	the gut microbiota	184:201	Accumulating evidence has reported that the gut microbiota could play important roles in the occurrence and progression of colorectal cancer.					
31830158	7	70	theme	colitis	1007:1013	arg1	dysbiosis					1034:1042	colitis cancer-induced gut dysbiosis	1007:1042	colitis cancer-induced gut dysbiosis	1007:1042	Contrasted with control group, the addition of JP could ward off colon cancer by ameliorating colitis cancer-induced gut dysbiosis.					
31830158	1	71	theme	cancer	124:129	arg1	model					137:141	a colorectal cancer mouse model	111:141	a colorectal cancer mouse model	111:141	polysaccharides in a colorectal cancer mouse model.					
31830158	6	72	theme	modulatory	681:690	arg1	effects					692:698	the modulatory effects	677:698	the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota	677:754	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	6	73	theme	AOM/DSS-induced	835:849	arg1	model					872:876	an AOM/DSS-induced colitis cancer mouse model	832:876	an AOM/DSS-induced colitis cancer mouse model	832:876	The aim of this study was to investigate the modulatory effects of jujube polysaccharides (JP) on intestinal microbiota, and the influence of JP on the gut flora structure was then analyzed using an AOM/DSS-induced colitis cancer mouse model, using high-throughput sequencing.					
31830158	5	74	theme	molecular	534:542	arg1	mechanisms					544:553	the molecular mechanisms	530:553	the molecular mechanisms involved in polysaccharides preventing carcinogenesis	530:607	However, the molecular mechanisms involved in polysaccharides preventing carcinogenesis are still uncharacterized.					
31830158	5	74	theme	molecular	534:542	arg1	uncharacterized					619:633	uncharacterized	619:633	uncharacterized	619:633	However, the molecular mechanisms involved in polysaccharides preventing carcinogenesis are still uncharacterized.					
31830158	1	75	theme	mouse	131:135	arg1	model					137:141	a colorectal cancer mouse model	111:141	a colorectal cancer mouse model	111:141	polysaccharides in a colorectal cancer mouse model.					
31830158	11	76	theme	appreciable	1563:1573	arg1	capability					1575:1584	an appreciable capability	1560:1584	an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS	1560:1647	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	0	77	theme	jujube	61:66	arg1	effects					50:56	gut microbiota modulatory effects	24:56	gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)	24:90	Metagenomic analysis of gut microbiota modulatory effects of jujube (Ziziphus jujuba Mill.)					
31830158	11	78	theme	JP	1589:1590	arg1	capability					1575:1584	an appreciable capability	1560:1584	an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS	1560:1647	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
31830158	11	79	dep	prevention	1743:1752	arg1	the					1739:1741	the	1739:1741	the	1739:1741	In conclusion, our results demonstrated an appreciable capability of JP to restore the gut microbiota profile altered by AOM/DSS, indicating the potential of jujube polysaccharides as promising prebiotic candidates for the prevention and treatment of colorectal cancer.					
33482213	1	0	theme	Ayurveda	384:391	arg1	Ashwagandharishta					355:371	Ashwagandharishta	355:371	Ashwagandharishta	355:371	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	1	0	theme	Ayurveda	384:391	arg1	medicine					345:352	the polyherbal fermented traditional medicine	308:352	the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda	308:391	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	3	1	dep	infrared	623:630	arg1	FT-IR					633:637	FT-IR	633:637	FT-IR	633:637	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	3	2	theme	carbohydrate	732:743	arg1	polymer					745:751	typical carbohydrate polymer functional groups and structural units	724:790	polymer	745:751	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	2	3	theme	liquid	411:416	arg1	chromatography					418:431	High-performance liquid chromatography	394:431	High-performance liquid chromatography (HPLC) based compositional analysis	394:467	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	2	3	theme	liquid	411:416	arg1	HPLC					434:437	HPLC	434:437	HPLC	434:437	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	3	4	theme	polymer	745:751	arg1	presence					712:719	the presence	708:719	the presence of typical carbohydrate polymer functional groups and structural units	708:790	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	5	5	theme	activities	1027:1036	arg1	activities					1027:1036	flocculation and emulsification activities	995:1036	flocculation and emulsification activities	995:1036	The purified EPS had shown 71.83% and 67.79% of flocculation and emulsification activities, respectively.					
33482213	5	5	theme	activities	1027:1036	arg1	%					979:979	71.83%	974:979	71.83%	974:979	The purified EPS had shown 71.83% and 67.79% of flocculation and emulsification activities, respectively.					
33482213	5	5	theme	activities	1027:1036	arg1	%					990:990	67.79%	985:990	67.79% of flocculation and emulsification activities	985:1036	The purified EPS had shown 71.83% and 67.79% of flocculation and emulsification activities, respectively.					
33482213	5	6	theme	emulsification	1012:1025	arg1	activities					1027:1036	flocculation and emulsification activities	995:1036	flocculation and emulsification activities	995:1036	The purified EPS had shown 71.83% and 67.79% of flocculation and emulsification activities, respectively.					
33482213	0	7	from	medicine	186:193	arg1	AG-06					135:139	probiotic Bacillus licheniformis AG-06	102:139	probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine	102:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	0	8	theme	probiotic	102:110	arg1	AG-06					135:139	probiotic Bacillus licheniformis AG-06	102:139	probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine	102:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	3	9	theme	units	786:790	arg1	presence					712:719	the presence	708:719	the presence of typical carbohydrate polymer functional groups and structural units	708:790	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	6	10	theme	dose-dependent	1287:1300	arg1	manner					1302:1307	a dose-dependent manner	1285:1307	a dose-dependent manner	1285:1307	Antioxidant activity was evaluated against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide, and superoxide free radicals and the scavenging actions were increased in a dose-dependent manner.					
33482213	7	11	theme	purified	1324:1331	arg1	EPS					1333:1335	the purified EPS	1320:1335	the purified EPS	1320:1335	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	6	12	theme	Antioxidant	1053:1063	arg1	activity					1065:1072	Antioxidant activity	1053:1072	Antioxidant activity	1053:1072	Antioxidant activity was evaluated against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide, and superoxide free radicals and the scavenging actions were increased in a dose-dependent manner.					
33482213	6	13	theme	superoxide	1215:1224	arg1	radicals					1231:1238	superoxide free radicals	1215:1238	superoxide free radicals	1215:1238	Antioxidant activity was evaluated against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide, and superoxide free radicals and the scavenging actions were increased in a dose-dependent manner.					
33482213	4	14	theme	atomic	913:918	arg1	studies					938:944	scanning electron microscopic and atomic force microscopic studies	879:944	scanning electron microscopic and atomic force microscopic studies	879:944	The purified EPS demonstrates the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies.					
33482213	7	15	theme	carcinoma	1402:1410	arg1	A549					1419:1422	A549	1419:1422	A549	1419:1422	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	7	15	theme	carcinoma	1402:1410	arg1	cells					1412:1416	the human lung carcinoma cells	1387:1416	the human lung carcinoma cells (A549)	1387:1423	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	4	16	theme	web-like	841:848	arg1	nature					869:874	the web-like fibrous and porous nature	837:874	the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies	837:944	The purified EPS demonstrates the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies.					
33482213	4	17	theme	fibrous	850:856	arg1	nature					869:874	the web-like fibrous and porous nature	837:874	the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies	837:944	The purified EPS demonstrates the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies.					
33482213	0	18	theme	licheniformis	121:133	arg1	AG-06					135:139	probiotic Bacillus licheniformis AG-06	102:139	probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine	102:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	0	19	theme	Structural	0:9	arg1	activities					55:64	functional and biological activities	29:64	functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine	29:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	0	19	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	2	20	theme	compositional	446:458	arg1	analysis					460:467	compositional analysis	446:467	compositional analysis	446:467	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	3	21	theme	structural	775:784	arg1	units					786:790	structural units	775:790	structural units	775:790	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	4	22	theme	microscopic	926:936	arg1	studies					938:944	scanning electron microscopic and atomic force microscopic studies	879:944	scanning electron microscopic and atomic force microscopic studies	879:944	The purified EPS demonstrates the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies.					
33482213	7	23	attach	derived	1486:1492	arg1	AG-06					1516:1520	AG-06	1516:1520	AG-06	1516:1520	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	7	23	attach	derived	1486:1492	arg2	EPS					1482:1484	the EPS	1478:1484	the EPS derived from B. licheniformis AG-06	1478:1520	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	3	24	theme	infrared	623:630	arg1	analyses					691:698	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses	605:698	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses	605:698	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	2	25	theme	EPS	512:514	arg1	units					598:602	the monomeric units	584:602	the monomeric units	584:602	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	2	25	theme	EPS	512:514	arg1	nature					498:503	the heteropolymeric nature	478:503	the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose	478:578	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	1	26	theme	polyherbal	312:321	arg1	Ashwagandharishta					355:371	Ashwagandharishta	355:371	Ashwagandharishta	355:371	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	1	26	theme	polyherbal	312:321	arg1	medicine					345:352	the polyherbal fermented traditional medicine	308:352	the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda	308:391	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	4	27	theme	force	920:924	arg1	studies					938:944	scanning electron microscopic and atomic force microscopic studies	879:944	scanning electron microscopic and atomic force microscopic studies	879:944	The purified EPS demonstrates the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies.					
33482213	5	28	theme	flocculation	995:1006	arg1	activities					1027:1036	flocculation and emulsification activities	995:1036	flocculation and emulsification activities	995:1036	The purified EPS had shown 71.83% and 67.79% of flocculation and emulsification activities, respectively.					
33482213	5	29	theme	purified	951:958	arg1	EPS					960:962	The purified EPS	947:962	The purified EPS	947:962	The purified EPS had shown 71.83% and 67.79% of flocculation and emulsification activities, respectively.					
33482213	0	30	theme	functional	29:38	arg1	activities					55:64	functional and biological activities	29:64	functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine	29:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	0	30	theme	functional	29:38	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	1	31	theme	fermented	323:331	arg1	Ashwagandharishta					355:371	Ashwagandharishta	355:371	Ashwagandharishta	355:371	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	1	31	theme	fermented	323:331	arg1	medicine					345:352	the polyherbal fermented traditional medicine	308:352	the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda	308:391	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	0	32	theme	Indian	146:151	arg1	medicine					186:193	Indian polyherbal fermented traditional medicine	146:193	Indian polyherbal fermented traditional medicine	146:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	6	33	theme	free	1226:1229	arg1	radicals					1231:1238	superoxide free radicals	1215:1238	superoxide free radicals	1215:1238	Antioxidant activity was evaluated against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide, and superoxide free radicals and the scavenging actions were increased in a dose-dependent manner.					
33482213	4	34	theme	purified	811:818	arg1	EPS					820:822	The purified EPS	807:822	The purified EPS	807:822	The purified EPS demonstrates the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies.					
33482213	7	35	theme	lung	1397:1400	arg1	A549					1419:1422	A549	1419:1422	A549	1419:1422	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	7	35	theme	lung	1397:1400	arg1	cells					1412:1416	the human lung carcinoma cells	1387:1416	the human lung carcinoma cells (A549)	1387:1423	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	1	36	theme	traditional	333:343	arg1	Ashwagandharishta					355:371	Ashwagandharishta	355:371	Ashwagandharishta	355:371	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	1	36	theme	traditional	333:343	arg1	medicine					345:352	the polyherbal fermented traditional medicine	308:352	the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda	308:391	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	2	37	theme	monomeric	588:596	arg1	units					598:602	the monomeric units	584:602	the monomeric units	584:602	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	2	37	theme	monomeric	588:596	arg1	nature					498:503	the heteropolymeric nature	478:503	the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose	478:578	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	3	38	theme	Fourier-transform	605:621	arg1	analyses					691:698	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses	605:698	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses	605:698	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	2	39	theme	heteropolymeric	482:496	arg1	units					598:602	the monomeric units	584:602	the monomeric units	584:602	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	2	39	theme	heteropolymeric	482:496	arg1	nature					498:503	the heteropolymeric nature	478:503	the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose	478:578	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	0	40	theme	biological	44:53	arg1	activities					55:64	functional and biological activities	29:64	functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine	29:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	0	40	theme	biological	44:53	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	0	41	theme	fermented	164:172	arg1	medicine					186:193	Indian polyherbal fermented traditional medicine	146:193	Indian polyherbal fermented traditional medicine	146:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	6	42	theme	3-ethylbenzothiazoline-6-sulfonic	1149:1181	arg1	2,2'-azino-bis					1134:1147	2,2'-azino-bis	1134:1147	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)	1134:1194	Antioxidant activity was evaluated against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide, and superoxide free radicals and the scavenging actions were increased in a dose-dependent manner.					
33482213	6	42	theme	3-ethylbenzothiazoline-6-sulfonic	1149:1181	arg1	acid					1183:1186	3-ethylbenzothiazoline-6-sulfonic acid	1149:1186	3-ethylbenzothiazoline-6-sulfonic acid	1149:1186	Antioxidant activity was evaluated against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide, and superoxide free radicals and the scavenging actions were increased in a dose-dependent manner.					
33482213	0	43	theme	polyherbal	153:162	arg1	medicine					186:193	Indian polyherbal fermented traditional medicine	146:193	Indian polyherbal fermented traditional medicine	146:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	3	44	theme	nuclear	644:650	arg1	resonance					661:669	nuclear magnetic resonance	644:669	nuclear magnetic resonance (NMR)	644:675	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	3	44	theme	nuclear	644:650	arg1	NMR					672:674	NMR	672:674	NMR	672:674	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	7	45	theme	anticancer	1454:1463	arg1	potential					1465:1473	the anticancer potential	1450:1473	the anticancer potential of the EPS derived from B. licheniformis AG-06	1450:1520	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	7	46	theme	human	1391:1395	arg1	A549					1419:1422	A549	1419:1422	A549	1419:1422	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	7	46	theme	human	1391:1395	arg1	cells					1412:1416	the human lung carcinoma cells	1387:1416	the human lung carcinoma cells (A549)	1387:1423	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	1	47	dep	bacterium	255:263	arg1	licheniformis					274:286	the probiotic bacterium Bacillus licheniformis	241:286	the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda	241:391	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	1	47	dep	bacterium	255:263	arg1	AG-06					288:292	AG-06	288:292	AG-06	288:292	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	4	48	theme	microscopic	897:907	arg1	studies					938:944	scanning electron microscopic and atomic force microscopic studies	879:944	scanning electron microscopic and atomic force microscopic studies	879:944	The purified EPS demonstrates the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies.					
33482213	2	49	theme	High-performance	394:409	arg1	chromatography					418:431	High-performance liquid chromatography	394:431	High-performance liquid chromatography (HPLC) based compositional analysis	394:467	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	2	49	theme	High-performance	394:409	arg1	HPLC					434:437	HPLC	434:437	HPLC	434:437	High-performance liquid chromatography (HPLC) based compositional analysis exhibits the heteropolymeric nature of the EPS consisting of galactose, rhamnose, xylose, mannose, and glucose, as the monomeric units.					
33482213	0	50	theme	traditional	174:184	arg1	medicine					186:193	Indian polyherbal fermented traditional medicine	146:193	Indian polyherbal fermented traditional medicine	146:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	3	51	theme	spectroscopic	677:689	arg1	analyses					691:698	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses	605:698	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses	605:698	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	7	52	theme	EPS	1482:1484	arg1	potential					1465:1473	the anticancer potential	1450:1473	the anticancer potential of the EPS derived from B. licheniformis AG-06	1450:1520	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	3	53	theme	typical	724:730	arg1	polymer					745:751	typical carbohydrate polymer functional groups and structural units	724:790	polymer	745:751	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	1	54	attach	isolated	294:301	arg1	Ashwagandharishta					355:371	Ashwagandharishta	355:371	Ashwagandharishta	355:371	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	1	54	attach	isolated	294:301	arg1	medicine					345:352	the polyherbal fermented traditional medicine	308:352	the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda	308:391	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	1	54	attach	isolated	294:301	arg2	bacterium					255:263	the probiotic bacterium Bacillus licheniformis	241:286	the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda	241:391	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	7	55	theme	significant	1348:1358	arg1	activity					1370:1377	a significant cytotoxic activity	1346:1377	a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06	1346:1520	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	1	56	theme	probiotic	245:253	arg1	bacterium					255:263	the probiotic bacterium Bacillus licheniformis	241:286	the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda	241:391	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	6	57	theme	scavenging	1248:1257	arg1	actions					1259:1265	the scavenging actions	1244:1265	the scavenging actions	1244:1265	Antioxidant activity was evaluated against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide, and superoxide free radicals and the scavenging actions were increased in a dose-dependent manner.					
33482213	3	58	dep	polymer	745:751	arg1	groups					764:769	functional groups	753:769	functional groups	753:769	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	4	59	from	nature	869:874	arg1	studies					938:944	scanning electron microscopic and atomic force microscopic studies	879:944	scanning electron microscopic and atomic force microscopic studies	879:944	The purified EPS demonstrates the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies.					
33482213	7	60	theme	cytotoxic	1360:1368	arg1	activity					1370:1377	a significant cytotoxic activity	1346:1377	a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06	1346:1520	Moreover, the purified EPS exhibits a significant cytotoxic activity against the human lung carcinoma cells (A549), which strongly suggests the anticancer potential of the EPS derived from B. licheniformis AG-06.					
33482213	0	61	theme	exopolysaccharide	72:88	arg1	activities					55:64	functional and biological activities	29:64	functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine	29:193	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	0	61	theme	exopolysaccharide	72:88	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization, functional and biological activities of an exopolysaccharide produced by probiotic Bacillus licheniformis AG-06 from Indian polyherbal fermented traditional medicine.					
33482213	3	62	theme	magnetic	652:659	arg1	resonance					661:669	nuclear magnetic resonance	644:669	nuclear magnetic resonance (NMR)	644:675	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	3	62	theme	magnetic	652:659	arg1	NMR					672:674	NMR	672:674	NMR	672:674	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	6	63	theme	nitric	1197:1202	arg1	oxide					1204:1208	nitric oxide	1197:1208	nitric oxide	1197:1208	Antioxidant activity was evaluated against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide, and superoxide free radicals and the scavenging actions were increased in a dose-dependent manner.					
33482213	1	64	theme	Bacillus	265:272	arg1	bacterium					255:263	the probiotic bacterium Bacillus licheniformis	241:286	the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda	241:391	An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus licheniformis AG-06 isolated from the polyherbal fermented traditional medicine (Ashwagandharishta) of Indian Ayurveda.					
33482213	3	65	theme	functional	753:762	arg1	groups					764:769	functional groups	753:769	functional groups	753:769	Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analyses confirm the presence of typical carbohydrate polymer functional groups and structural units, respectively.					
33482213	4	66	theme	porous	862:867	arg1	nature					869:874	the web-like fibrous and porous nature	837:874	the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies	837:944	The purified EPS demonstrates the web-like fibrous and porous nature in scanning electron microscopic and atomic force microscopic studies.					
34520778	0	0	theme	antioxidant	86:96	arg1	activities					98:107	antioxidant activities	86:107	antioxidant activities	86:107	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.					
34520778	4	1	theme	DE52	544:547	arg1	column					549:554	DE52 column	544:554	DE52 column	544:554	The polysaccharide was firstly isolated by ion-exchange chromatography equipped with DE52 column, followed by isolated through Superdex-200 column.					
34520778	10	2	theme	P.	1207:1208	arg1	polysaccharide					1220:1233	P. sibiricum polysaccharide	1207:1233	P. sibiricum polysaccharide	1207:1233	Together, P. sibiricum polysaccharide has the potential to be a natural antioxidant.					
34520778	9	3	contain	had	1159:1161	arg1	polysaccharides					1080:1094	PSPJW polysaccharides	1074:1094	PSPJW polysaccharides with different molecular weight and similar chemical structure	1074:1157	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	9	3	contain	had	1159:1161	arg2	activities					1185:1194	different antioxidant activities	1163:1194	different antioxidant activities	1163:1194	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	2	4	from	Exploration	200:210	arg1	species					248:254	Polygonatum species	236:254	Polygonatum species	236:254	Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities.					
34520778	0	5	from	elucidation	9:19	arg1	rhizome					48:54	rhizome	48:54	rhizome of Polygonatum sibiricum with antioxidant activities	48:107	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.					
34520778	5	6	theme	homogenous	642:651	arg1	one					653:655	a homogenous one	640:655	a homogenous one with average molecular weight of 141 kDa	640:696	The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa.					
34520778	5	6	theme	homogenous	642:651	arg1	fraction					627:634	The obtained PSPJWA fraction	607:634	The obtained PSPJWA fraction	607:634	The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa.					
34520778	1	7	theme	immunity	178:185	arg1	enhancement					187:197	immunity enhancement	178:197	immunity enhancement	178:197	Polygonatum sibiricum is traditionally used as Chinese medicine for immunity enhancement.					
34520778	7	8	dep	-Rhap-	942:947	arg1	Galp-					894:898	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	Galp-	894:898	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	8	dep	-Rhap-	942:947	arg1	→5					864:865	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	→5	864:865	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	8	dep	-Rhap-	942:947	arg1	→3,5					878:881	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	→3,5	878:881	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	8	dep	-Rhap-	942:947	arg1	residue					952:958	1→ residue	949:958	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue	854:958	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	8	dep	-Rhap-	942:947	arg1	1→					930:931	1→	930:931	1→	930:931	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	8	dep	-Rhap-	942:947	arg1	→2,4					937:940	→2,4	937:940	→2,4	937:940	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	8	dep	-Rhap-	942:947	arg1	→4					904:905	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	→4	904:905	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	8	dep	-Rhap-	942:947	arg1	→4,6					918:921	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	→4,6	918:921	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	9	9	theme	chemical	1140:1147	arg1	structure					1149:1157	similar chemical structure	1132:1157	similar chemical structure	1132:1157	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	10	10	contain	has	1235:1237	arg1	polysaccharide					1220:1233	P. sibiricum polysaccharide	1207:1233	P. sibiricum polysaccharide	1207:1233	Together, P. sibiricum polysaccharide has the potential to be a natural antioxidant.					
34520778	10	10	contain	has	1235:1237	arg2	potential					1243:1251	the potential to be a natural antioxidant	1239:1279	the potential to be a natural antioxidant	1239:1279	Together, P. sibiricum polysaccharide has the potential to be a natural antioxidant.					
34520778	9	11	theme	molecular	1111:1119	arg1	weight					1121:1126	different molecular weight	1101:1126	different molecular weight	1101:1126	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	6	12	from	rhamnose	759:766	arg1	ratio					773:777	a ratio	771:777	a ratio of 14:4:1	771:787	The monosaccharide composition was galactose, arabinose and rhamnose in a ratio of 14:4:1.					
34520778	7	13	theme	-Galp-	907:912	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	13	theme	-Galp-	907:912	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	2	14	theme	chemical	312:319	arg1	structures					321:330	their chemical structures	306:330	their chemical structures	306:330	Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities.					
34520778	7	15	theme	1→	874:875	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	15	theme	1→	874:875	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	2	16	from	species	248:254	arg1	Exploration					200:210	Exploration	200:210	Exploration of polysaccharides from Polygonatum species	200:254	Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities.					
34520778	2	16	from	species	248:254	arg1	polysaccharides					215:229	polysaccharides	215:229	polysaccharides from Polygonatum species	215:254	Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities.					
34520778	3	17	from	sibiricum	416:424	arg1	polysaccharide					393:406	the alkali-extracted polysaccharide	372:406	the alkali-extracted polysaccharide from P. sibiricum (PSP)	372:430	In this study, the alkali-extracted polysaccharide from P. sibiricum (PSP) was isolated and examined.					
34520778	5	18	theme	molecular	670:678	arg1	weight					680:685	average molecular weight	662:685	average molecular weight of 141 kDa	662:696	The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa.					
34520778	5	19	theme	average	662:668	arg1	weight					680:685	average molecular weight	662:685	average molecular weight of 141 kDa	662:696	The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa.					
34520778	4	20	theme	ion-exchange	502:513	arg1	chromatography					515:528	ion-exchange chromatography	502:528	ion-exchange chromatography equipped with DE52 column, followed by isolated through Superdex-200 column	502:604	The polysaccharide was firstly isolated by ion-exchange chromatography equipped with DE52 column, followed by isolated through Superdex-200 column.					
34520778	7	21	theme	1→	890:891	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	21	theme	1→	890:891	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	9	22	theme	different	1101:1109	arg1	weight					1121:1126	different molecular weight	1101:1126	different molecular weight	1101:1126	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	3	23	theme	alkali-extracted	376:391	arg1	polysaccharide					393:406	the alkali-extracted polysaccharide	372:406	the alkali-extracted polysaccharide from P. sibiricum (PSP)	372:430	In this study, the alkali-extracted polysaccharide from P. sibiricum (PSP) was isolated and examined.					
34520778	5	24	theme	PSPJWA	620:625	arg1	one					653:655	a homogenous one	640:655	a homogenous one with average molecular weight of 141 kDa	640:696	The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa.					
34520778	5	24	theme	PSPJWA	620:625	arg1	fraction					627:634	The obtained PSPJWA fraction	607:634	The obtained PSPJWA fraction	607:634	The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa.					
34520778	7	25	theme	-Araf-	867:872	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	25	theme	-Araf-	867:872	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	8	26	theme	arabinogalactan-type	1042:1061	arg1	structure					1063:1071	a novel arabinogalactan-type structure	1034:1071	a novel arabinogalactan-type structure	1034:1071	NMR and enzymatic studies showed that PSPJWA was a novel arabinogalactan-type structure.					
34520778	8	26	theme	arabinogalactan-type	1042:1061	arg1	PSPJWA					1023:1028	PSPJWA	1023:1028	PSPJWA	1023:1028	NMR and enzymatic studies showed that PSPJWA was a novel arabinogalactan-type structure.					
34520778	8	27	theme	novel	1036:1040	arg1	structure					1063:1071	a novel arabinogalactan-type structure	1034:1071	a novel arabinogalactan-type structure	1034:1071	NMR and enzymatic studies showed that PSPJWA was a novel arabinogalactan-type structure.					
34520778	8	27	theme	novel	1036:1040	arg1	PSPJWA					1023:1028	PSPJWA	1023:1028	PSPJWA	1023:1028	NMR and enzymatic studies showed that PSPJWA was a novel arabinogalactan-type structure.					
34520778	6	28	from	galactose	734:742	arg1	ratio					773:777	a ratio	771:777	a ratio of 14:4:1	771:787	The monosaccharide composition was galactose, arabinose and rhamnose in a ratio of 14:4:1.					
34520778	0	29	from	rhizome	48:54	arg1	elucidation					9:19	Chemical elucidation	0:19	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.	0:108	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.					
34520778	0	29	from	rhizome	48:54	arg1	arabinogalactan					27:41	an arabinogalactan	24:41	an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities	24:107	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.					
34520778	0	30	theme	Chemical	0:7	arg1	elucidation					9:19	Chemical elucidation	0:19	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.	0:108	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.					
34520778	7	31	theme	Araf-	854:858	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	31	theme	Araf-	854:858	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	5	32	theme	141 kDa	690:696	arg1	weight					680:685	average molecular weight	662:685	average molecular weight of 141 kDa	662:696	The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa.					
34520778	7	33	theme	1→	900:901	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	33	theme	1→	900:901	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	1	34	used	used	149:152	arg2	sibiricum					122:130	Polygonatum sibiricum	110:130	Polygonatum sibiricum	110:130	Polygonatum sibiricum is traditionally used as Chinese medicine for immunity enhancement.					
34520778	1	34	used	used	149:152	arg2	medicine					165:172	Chinese medicine	157:172	Chinese medicine	157:172	Polygonatum sibiricum is traditionally used as Chinese medicine for immunity enhancement.					
34520778	10	35	theme	natural	1261:1267	arg1	antioxidant					1269:1279	a natural antioxidant	1259:1279	a natural antioxidant	1259:1279	Together, P. sibiricum polysaccharide has the potential to be a natural antioxidant.					
34520778	2	36	theme	function	336:343	arg1	activities					345:354	function activities	336:354	function activities	336:354	Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities.					
34520778	7	37	theme	1→	914:915	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	37	theme	1→	914:915	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	6	38	theme	14:4:1	782:787	arg1	ratio					773:777	a ratio	771:777	a ratio of 14:4:1	771:787	The monosaccharide composition was galactose, arabinose and rhamnose in a ratio of 14:4:1.					
34520778	9	39	theme	different	1163:1171	arg1	activities					1185:1194	different antioxidant activities	1163:1194	different antioxidant activities	1163:1194	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	2	40	from	studies	295:301	arg1	structures					321:330	their chemical structures	306:330	their chemical structures	306:330	Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities.					
34520778	2	40	from	studies	295:301	arg1	activities					345:354	function activities	336:354	function activities	336:354	Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities.					
34520778	10	41	theme	sibiricum	1210:1218	arg1	polysaccharide					1220:1233	P. sibiricum polysaccharide	1207:1233	P. sibiricum polysaccharide	1207:1233	Together, P. sibiricum polysaccharide has the potential to be a natural antioxidant.					
34520778	9	42	theme	antioxidant	1173:1183	arg1	activities					1185:1194	different antioxidant activities	1163:1194	different antioxidant activities	1163:1194	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	2	43	theme	wider	272:276	arg1	insight					278:284	a wider insight	270:284	a wider insight into the studies on their chemical structures and function activities	270:354	Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities.					
34520778	0	44	theme	arabinogalactan	27:41	arg1	elucidation					9:19	Chemical elucidation	0:19	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.	0:108	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.					
34520778	7	45	theme	methylation	963:973	arg1	analysis					975:982	methylation analysis	963:982	methylation analysis	963:982	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	46	theme	fraction	824:831	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	46	theme	fraction	824:831	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	8	47	theme	enzymatic	993:1001	arg1	studies					1003:1009	enzymatic studies	993:1009	enzymatic studies	993:1009	NMR and enzymatic studies showed that PSPJWA was a novel arabinogalactan-type structure.					
34520778	7	48	theme	PSPJWA	817:822	arg1	fraction					824:831	PSPJWA fraction	817:831	PSPJWA fraction	817:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	5	49	with	one	653:655	arg1	weight					680:685	average molecular weight	662:685	average molecular weight of 141 kDa	662:696	The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa.					
34520778	6	50	from	arabinose	745:753	arg1	ratio					773:777	a ratio	771:777	a ratio of 14:4:1	771:787	The monosaccharide composition was galactose, arabinose and rhamnose in a ratio of 14:4:1.					
34520778	2	51	theme	Polygonatum	236:246	arg1	species					248:254	Polygonatum species	236:254	Polygonatum species	236:254	Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities.					
34520778	5	52	theme	obtained	611:618	arg1	one					653:655	a homogenous one	640:655	a homogenous one with average molecular weight of 141 kDa	640:696	The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa.					
34520778	5	52	theme	obtained	611:618	arg1	fraction					627:634	The obtained PSPJWA fraction	607:634	The obtained PSPJWA fraction	607:634	The obtained PSPJWA fraction was a homogenous one with average molecular weight of 141 kDa.					
34520778	9	53	theme	similar	1132:1138	arg1	structure					1149:1157	similar chemical structure	1132:1157	similar chemical structure	1132:1157	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	1	54	theme	Polygonatum	110:120	arg1	medicine					165:172	Chinese medicine	157:172	Chinese medicine	157:172	Polygonatum sibiricum is traditionally used as Chinese medicine for immunity enhancement.					
34520778	1	54	theme	Polygonatum	110:120	arg1	sibiricum					122:130	Polygonatum sibiricum	110:130	Polygonatum sibiricum	110:130	Polygonatum sibiricum is traditionally used as Chinese medicine for immunity enhancement.					
34520778	9	55	with	polysaccharides	1080:1094	arg1	weight					1121:1126	different molecular weight	1101:1126	different molecular weight	1101:1126	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	9	55	with	polysaccharides	1080:1094	arg1	structure					1149:1157	similar chemical structure	1132:1157	similar chemical structure	1132:1157	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	9	56	theme	PSPJW	1074:1078	arg1	polysaccharides					1080:1094	PSPJW polysaccharides	1074:1094	PSPJW polysaccharides with different molecular weight and similar chemical structure	1074:1157	PSPJW polysaccharides with different molecular weight and similar chemical structure had different antioxidant activities.					
34520778	7	57	theme	-Galp-	923:928	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	57	theme	-Galp-	923:928	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	58	theme	1→	860:861	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	58	theme	1→	860:861	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	0	59	theme	sibiricum	71:79	arg1	rhizome					48:54	rhizome	48:54	rhizome of Polygonatum sibiricum with antioxidant activities	48:107	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.					
34520778	2	60	theme	polysaccharides	215:229	arg1	Exploration					200:210	Exploration	200:210	Exploration of polysaccharides from Polygonatum species	200:254	Exploration of polysaccharides from Polygonatum species would provide a wider insight into the studies on their chemical structures and function activities.					
34520778	0	61	with	rhizome	48:54	arg1	activities					98:107	antioxidant activities	86:107	antioxidant activities	86:107	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.					
34520778	1	62	theme	Chinese	157:163	arg1	medicine					165:172	Chinese medicine	157:172	Chinese medicine	157:172	Polygonatum sibiricum is traditionally used as Chinese medicine for immunity enhancement.					
34520778	1	62	theme	Chinese	157:163	arg1	sibiricum					122:130	Polygonatum sibiricum	110:130	Polygonatum sibiricum	110:130	Polygonatum sibiricum is traditionally used as Chinese medicine for immunity enhancement.					
34520778	6	63	theme	monosaccharide	703:716	arg1	galactose					734:742	galactose	734:742	galactose	734:742	The monosaccharide composition was galactose, arabinose and rhamnose in a ratio of 14:4:1.					
34520778	6	63	theme	monosaccharide	703:716	arg1	composition					718:728	The monosaccharide composition	699:728	The monosaccharide composition	699:728	The monosaccharide composition was galactose, arabinose and rhamnose in a ratio of 14:4:1.					
34520778	0	64	theme	Polygonatum	59:69	arg1	sibiricum					71:79	Polygonatum sibiricum	59:79	Polygonatum sibiricum	59:79	Chemical elucidation of an arabinogalactan from rhizome of Polygonatum sibiricum with antioxidant activities.					
34520778	7	65	theme	-Araf-	883:888	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	65	theme	-Araf-	883:888	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	66	theme	glycosidic	794:803	arg1	-Rhap-					942:947	Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-	854:947	-Rhap-	942:947	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34520778	7	66	theme	glycosidic	794:803	arg1	linkages					805:812	The glycosidic linkages	790:812	The glycosidic linkages of PSPJWA fraction	790:831	The glycosidic linkages of PSPJWA fraction were indicated to be Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, Galp-(1→, →4)-Galp-(1→, →4,6)-Galp-(1→ and →2,4)-Rhap-(1→ residue by methylation analysis.					
34009446	5	0	theme	monosaccharide	606:619	arg1	composition					621:631	The monosaccharide composition	602:631	The monosaccharide composition	602:631	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	1	1	theme	many	182:185	arg1	niches					208:213	many different ecological niches	182:213	many different ecological niches	182:213	Bacteria belonging to the genus Herbaspirillum are found in many different ecological niches.					
34009446	0	2	theme	ecological	97:106	arg1	environments					108:119	diverse ecological environments	89:119	diverse ecological environments	89:119	Differential exopolysaccharide production and composition by Herbaspirillum strains from diverse ecological environments.					
34009446	4	3	theme	ecological	574:583	arg1	characteristics					585:599	distinct ecological characteristics	565:599	distinct ecological characteristics	565:599	We have analyzed the production of exopolysaccharides (EPS) by Herbaspirillum strains isolated from different sources and with distinct ecological characteristics.					
34009446	5	4	theme	selected	673:680	arg1	strains					682:688	selected strains	673:688	selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp	673:830	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	5	4	theme	selected	673:680	arg1	relationship					761:772	the relationship	757:772	the relationship with the ecological niches occupied by Herbaspirillum spp	757:830	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	5	4	theme	selected	673:680	arg1	isolates					743:750	free-living, plant-associated and clinical isolates	700:750	free-living, plant-associated and clinical isolates	700:750	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	1	5	theme	different	187:195	arg1	niches					208:213	many different ecological niches	182:213	many different ecological niches	182:213	Bacteria belonging to the genus Herbaspirillum are found in many different ecological niches.					
34009446	4	6	theme	distinct	565:572	arg1	characteristics					585:599	distinct ecological characteristics	565:599	distinct ecological characteristics	565:599	We have analyzed the production of exopolysaccharides (EPS) by Herbaspirillum strains isolated from different sources and with distinct ecological characteristics.					
34009446	2	7	theme	various	319:325	arg1	environments					327:338	various environments	319:338	various environments	319:338	Some species are typically endophytic, while others were reported as free-living organisms that occupy various environments.					
34009446	1	8	located	found	173:177	arg2	Bacteria					122:129	Bacteria	122:129	Bacteria belonging to the genus Herbaspirillum	122:167	Bacteria belonging to the genus Herbaspirillum are found in many different ecological niches.					
34009446	1	8	located	found	173:177	arg1	niches					208:213	many different ecological niches	182:213	many different ecological niches	182:213	Bacteria belonging to the genus Herbaspirillum are found in many different ecological niches.					
34009446	5	9	theme	free-living	700:710	arg1	isolates					743:750	free-living, plant-associated and clinical isolates	700:750	free-living, plant-associated and clinical isolates	700:750	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	1	10	theme	ecological	197:206	arg1	niches					208:213	many different ecological niches	182:213	many different ecological niches	182:213	Bacteria belonging to the genus Herbaspirillum are found in many different ecological niches.					
34009446	5	11	theme	plant-associated	713:728	arg1	isolates					743:750	free-living, plant-associated and clinical isolates	700:750	free-living, plant-associated and clinical isolates	700:750	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	0	12	theme	exopolysaccharide	13:29	arg1	production					31:40	Differential exopolysaccharide production	0:40	Differential exopolysaccharide production	0:40	Differential exopolysaccharide production and composition by Herbaspirillum strains from diverse ecological environments.					
34009446	4	13	theme	different	538:546	arg1	sources					548:554	different sources	538:554	different sources	538:554	We have analyzed the production of exopolysaccharides (EPS) by Herbaspirillum strains isolated from different sources and with distinct ecological characteristics.					
34009446	5	14	with	relationship	761:772	arg1	niches					794:799	the ecological niches	779:799	the ecological niches occupied by Herbaspirillum spp	779:830	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	0	15	theme	Differential	0:11	arg1	production					31:40	Differential exopolysaccharide production	0:40	Differential exopolysaccharide production	0:40	Differential exopolysaccharide production and composition by Herbaspirillum strains from diverse ecological environments.					
34009446	5	16	theme	clinical	734:741	arg1	isolates					743:750	free-living, plant-associated and clinical isolates	700:750	free-living, plant-associated and clinical isolates	700:750	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	3	17	theme	several	420:426	arg1	diseases					428:435	several diseases	420:435	several diseases	420:435	Also, opportunistic herbaspirilli have been found infecting humans affected by several diseases.					
34009446	5	18	with	isolates	743:750	arg1	niches					794:799	the ecological niches	779:799	the ecological niches occupied by Herbaspirillum spp	779:830	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	5	19	theme	Herbaspirillum	813:826	arg1	spp					828:830	Herbaspirillum spp	813:830	Herbaspirillum spp	813:830	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	4	20	theme	Herbaspirillum	501:514	arg1	strains					516:522	Herbaspirillum strains	501:522	Herbaspirillum strains isolated from different sources and with distinct ecological characteristics	501:599	We have analyzed the production of exopolysaccharides (EPS) by Herbaspirillum strains isolated from different sources and with distinct ecological characteristics.					
34009446	3	21	theme	opportunistic	347:359	arg1	herbaspirilli					361:373	opportunistic herbaspirilli	347:373	opportunistic herbaspirilli	347:373	Also, opportunistic herbaspirilli have been found infecting humans affected by several diseases.					
34009446	4	22	theme	exopolysaccharides	473:490	arg1	production					459:468	the production	455:468	the production of exopolysaccharides (EPS)	455:496	We have analyzed the production of exopolysaccharides (EPS) by Herbaspirillum strains isolated from different sources and with distinct ecological characteristics.					
34009446	1	23	theme	genus	148:152	arg1	Herbaspirillum					154:167	the genus Herbaspirillum	144:167	the genus Herbaspirillum	144:167	Bacteria belonging to the genus Herbaspirillum are found in many different ecological niches.					
34009446	5	24	theme	ecological	783:792	arg1	niches					794:799	the ecological niches	779:799	the ecological niches occupied by Herbaspirillum spp	779:830	The monosaccharide composition was determined for the EPS obtained for selected strains including free-living, plant-associated and clinical isolates, and the relationship with the ecological niches occupied by Herbaspirillum spp.					
34009446	2	25	theme	free-living	285:295	arg1	others					261:266	others	261:266	others	261:266	Some species are typically endophytic, while others were reported as free-living organisms that occupy various environments.					
34009446	2	25	theme	free-living	285:295	arg1	organisms					297:305	free-living organisms	285:305	free-living organisms that occupy various environments	285:338	Some species are typically endophytic, while others were reported as free-living organisms that occupy various environments.					
34009446	0	26	theme	diverse	89:95	arg1	environments					108:119	diverse ecological environments	89:119	diverse ecological environments	89:119	Differential exopolysaccharide production and composition by Herbaspirillum strains from diverse ecological environments.					
34009446	4	27	attach	isolated	524:531	arg2	strains					516:522	Herbaspirillum strains	501:522	Herbaspirillum strains isolated from different sources and with distinct ecological characteristics	501:599	We have analyzed the production of exopolysaccharides (EPS) by Herbaspirillum strains isolated from different sources and with distinct ecological characteristics.					
34009446	4	27	attach	isolated	524:531	arg1	sources					548:554	different sources	538:554	different sources	538:554	We have analyzed the production of exopolysaccharides (EPS) by Herbaspirillum strains isolated from different sources and with distinct ecological characteristics.					
32131450	6	0	theme	steric	1392:1397	arg1	restrictions					1399:1410	steric restrictions	1392:1410	steric restrictions	1392:1410	Both modelling and NMR data pointed at restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions.					
32131450	7	1	theme	dynamics	1514:1521	arg1	simulations					1523:1533	molecular dynamics simulations	1504:1533	molecular dynamics simulations	1504:1533	In addition, the good correlation between NOE data and calculated interatomic distances by molecular dynamics simulations validated potential energy functions used for calculations.					
32131450	6	2	theme	local	1297:1301	arg1	conformations					1303:1315	local conformations	1297:1315	local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions	1297:1410	Both modelling and NMR data pointed at restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions.					
32131450	1	3	theme	life	173:176	arg1	Biofilms					137:144	Biofilms	137:144	Biofilms	137:144	Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells.					
32131450	1	3	theme	life	173:176	arg1	way					166:168	a multicellular way	150:168	a multicellular way	150:168	Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells.					
32131450	4	4	theme	Gas-Liquid	621:630	arg1	GLC-MS					666:671	GLC-MS	666:671	GLC-MS	666:671	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	4	4	theme	Gas-Liquid	621:630	arg1	Spectrometry					652:663	Gas-Liquid Chromatography-Mass Spectrometry	621:663	Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS)	621:672	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	0	5	theme	Structural	87:96	arg1	Determination					98:110	Structural Determination	87:110	Structural Determination	87:110	Burkholderia cenocepacia H111 Produces a Water-Insoluble Exopolysaccharide in Biofilm: Structural Determination and Molecular Modelling.					
32131450	5	6	theme	NMR	1079:1081	arg1	data					1115:1118	NMR Nuclear Overhauser Effect (NOE) data	1079:1118	NMR Nuclear Overhauser Effect (NOE) data	1079:1118	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	5	7	theme	[3	972:973	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	3	8	theme	cystic	533:538	arg1	biofilm					473:479	the biofilm	469:479	the biofilm produced by Burkholderia cenocepacia strain H111	469:528	A water-insoluble polysaccharide was isolated and purified from the biofilm produced by Burkholderia cenocepacia strain H111, a cystic fibrosis pathogen.					
32131450	3	8	theme	cystic	533:538	arg1	pathogen					549:556	a cystic fibrosis pathogen	531:556	a cystic fibrosis pathogen	531:556	A water-insoluble polysaccharide was isolated and purified from the biofilm produced by Burkholderia cenocepacia strain H111, a cystic fibrosis pathogen.					
32131450	5	9	theme	-α-d-Glcp-	990:999	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	0	10	theme	Molecular	116:124	arg1	Modelling					126:134	Molecular Modelling	116:134	Molecular Modelling	116:134	Burkholderia cenocepacia H111 Produces a Water-Insoluble Exopolysaccharide in Biofilm: Structural Determination and Molecular Modelling.					
32131450	5	11	theme	-α-d-Galp-	1005:1014	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	5	12	theme	polysaccharide	1206:1219	arg1	insolubility					1221:1232	the polysaccharide insolubility	1202:1232	the polysaccharide insolubility	1202:1232	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	4	13	theme	sodium	801:806	arg1	hydroxide					808:816	deuterated sodium hydroxide	790:816	deuterated sodium hydroxide (NaOD) aqueous solutions	790:841	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	4	13	theme	sodium	801:806	arg1	NaOD					819:822	NaOD	819:822	NaOD	819:822	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	7	14	theme	calculated	1468:1477	arg1	distances					1491:1499	calculated interatomic distances	1468:1499	calculated interatomic distances	1468:1499	In addition, the good correlation between NOE data and calculated interatomic distances by molecular dynamics simulations validated potential energy functions used for calculations.					
32131450	6	15	theme	restricted	1274:1283	arg1	dynamics					1285:1292	restricted dynamics	1274:1292	restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions	1274:1410	Both modelling and NMR data pointed at restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions.					
32131450	4	16	dep	1D	760:761	arg1	spectroscopy					774:785	NMR spectroscopy	770:785	NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions	770:841	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	3	17	theme	strain	518:523	arg1	H111					525:528	Burkholderia cenocepacia strain H111	493:528	Burkholderia cenocepacia strain H111	493:528	A water-insoluble polysaccharide was isolated and purified from the biofilm produced by Burkholderia cenocepacia strain H111, a cystic fibrosis pathogen.					
32131450	6	18	theme	hydrogen	1370:1377	arg1	bonds					1379:1383	inter-residue hydrogen bonds	1356:1383	inter-residue hydrogen bonds	1356:1383	Both modelling and NMR data pointed at restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions.					
32131450	0	19	from	Exopolysaccharide	57:73	arg1	Biofilm					78:84	Biofilm	78:84	Biofilm	78:84	Burkholderia cenocepacia H111 Produces a Water-Insoluble Exopolysaccharide in Biofilm: Structural Determination and Molecular Modelling.					
32131450	4	20	theme	appropriate	677:687	arg1	derivatives					702:712	appropriate carbohydrate derivatives	677:712	appropriate carbohydrate derivatives	677:712	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	7	21	theme	good	1430:1433	arg1	correlation					1435:1445	the good correlation	1426:1445	the good correlation between NOE data and calculated interatomic distances by molecular dynamics simulations	1426:1533	In addition, the good correlation between NOE data and calculated interatomic distances by molecular dynamics simulations validated potential energy functions used for calculations.					
32131450	4	22	theme	complete	724:731	arg1	structure					733:741	its complete structure	720:741	its complete structure	720:741	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	4	23	theme	Chromatography-Mass	632:650	arg1	GLC-MS					666:671	GLC-MS	666:671	GLC-MS	666:671	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	4	23	theme	Chromatography-Mass	632:650	arg1	Spectrometry					652:663	Gas-Liquid Chromatography-Mass Spectrometry	621:663	Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS)	621:672	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	4	24	theme	aqueous	825:831	arg1	solutions					833:841	deuterated sodium hydroxide (NaOD) aqueous solutions	790:841	deuterated sodium hydroxide (NaOD) aqueous solutions	790:841	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	1	25	theme	bacterial	185:193	arg1	cells					195:199	bacterial cells	185:199	bacterial cells	185:199	Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells.					
32131450	0	26	theme	cenocepacia	13:23	arg1	H111					25:28	Burkholderia cenocepacia H111	0:28	Burkholderia cenocepacia H111	0:28	Burkholderia cenocepacia H111 Produces a Water-Insoluble Exopolysaccharide in Biofilm: Structural Determination and Molecular Modelling.					
32131450	2	27	theme	Extracellular	319:331	arg1	polysaccharides					333:347	Extracellular polysaccharides	319:347	Extracellular polysaccharides	319:347	Extracellular polysaccharides play an important role in matrix setup and maintenance.					
32131450	5	28	theme	NOE	1110:1112	arg1	data					1115:1118	NMR Nuclear Overhauser Effect (NOE) data	1079:1118	NMR Nuclear Overhauser Effect (NOE) data	1079:1118	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	5	29	theme	cenocepacia	936:946	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	0	30	theme	Burkholderia	0:11	arg1	H111					25:28	Burkholderia cenocepacia H111	0:28	Burkholderia cenocepacia H111	0:28	Burkholderia cenocepacia H111 Produces a Water-Insoluble Exopolysaccharide in Biofilm: Structural Determination and Molecular Modelling.					
32131450	7	31	theme	interatomic	1479:1489	arg1	distances					1491:1499	calculated interatomic distances	1468:1499	calculated interatomic distances	1468:1499	In addition, the good correlation between NOE data and calculated interatomic distances by molecular dynamics simulations validated potential energy functions used for calculations.					
32131450	6	32	theme	NMR	1254:1256	arg1	data					1258:1261	NMR data	1254:1261	NMR data	1254:1261	Both modelling and NMR data pointed at restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions.					
32131450	4	33	theme	deuterated	790:799	arg1	hydroxide					808:816	deuterated sodium hydroxide	790:816	deuterated sodium hydroxide (NaOD) aqueous solutions	790:841	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	4	33	theme	deuterated	790:799	arg1	NaOD					819:822	NaOD	819:822	NaOD	819:822	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	5	34	theme	biofilm	948:954	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	4	35	theme	glycosidic	579:588	arg1	linkages					590:597	glycosidic linkages	579:597	glycosidic linkages	579:597	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	4	36	theme	carbohydrate	689:700	arg1	derivatives					702:712	appropriate carbohydrate derivatives	677:712	appropriate carbohydrate derivatives	677:712	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	5	37	theme	following	884:892	arg1	unit					904:907	the following repeating unit	880:907	the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility	880:1232	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	1	38	theme	advantages	294:303	arg1	number					284:289	a number	282:289	a number of advantages to the cells	282:316	Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells.					
32131450	0	39	from	Determination	98:110	arg1	Biofilm					78:84	Biofilm	78:84	Biofilm	78:84	Burkholderia cenocepacia H111 Produces a Water-Insoluble Exopolysaccharide in Biofilm: Structural Determination and Molecular Modelling.					
32131450	4	40	theme	hydroxide	808:816	arg1	solutions					833:841	deuterated sodium hydroxide (NaOD) aqueous solutions	790:841	deuterated sodium hydroxide (NaOD) aqueous solutions	790:841	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	3	41	theme	Burkholderia	493:504	arg1	H111					525:528	Burkholderia cenocepacia strain H111	493:528	Burkholderia cenocepacia strain H111	493:528	A water-insoluble polysaccharide was isolated and purified from the biofilm produced by Burkholderia cenocepacia strain H111, a cystic fibrosis pathogen.					
32131450	0	42	theme	Water-Insoluble	41:55	arg1	Exopolysaccharide					57:73	a Water-Insoluble Exopolysaccharide	39:73	a Water-Insoluble Exopolysaccharide in Biofilm	39:84	Burkholderia cenocepacia H111 Produces a Water-Insoluble Exopolysaccharide in Biofilm: Structural Determination and Molecular Modelling.					
32131450	5	43	used	used	1060:1063	arg2	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	1	44	from	matrix	262:267	arg1	embedded					224:231	embedded	224:231	embedded	224:231	Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells.					
32131450	5	45	theme	Nuclear	1083:1089	arg1	data					1115:1118	NMR Nuclear Overhauser Effect (NOE) data	1079:1118	NMR Nuclear Overhauser Effect (NOE) data	1079:1118	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	5	46	theme	n	1034:1034	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	7	47	theme	NOE	1455:1457	arg1	data					1459:1462	NOE data	1455:1462	NOE data	1455:1462	In addition, the good correlation between NOE data and calculated interatomic distances by molecular dynamics simulations validated potential energy functions used for calculations.					
32131450	5	48	theme	water-insoluble	917:931	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	5	49	theme	Overhauser	1091:1100	arg1	data					1115:1118	NMR Nuclear Overhauser Effect (NOE) data	1079:1118	NMR Nuclear Overhauser Effect (NOE) data	1079:1118	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	5	50	theme	Molecular	1036:1044	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	6	51	theme	inter-residue	1356:1368	arg1	bonds					1379:1383	inter-residue hydrogen bonds	1356:1383	inter-residue hydrogen bonds	1356:1383	Both modelling and NMR data pointed at restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions.					
32131450	3	52	theme	water-insoluble	407:421	arg1	polysaccharide					423:436	A water-insoluble polysaccharide	405:436	A water-insoluble polysaccharide	405:436	A water-insoluble polysaccharide was isolated and purified from the biofilm produced by Burkholderia cenocepacia strain H111, a cystic fibrosis pathogen.					
32131450	5	53	theme	polysaccharide	956:969	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	5	54	theme	Effect	1102:1107	arg1	data					1115:1118	NMR Nuclear Overhauser Effect (NOE) data	1079:1118	NMR Nuclear Overhauser Effect (NOE) data	1079:1118	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	1	55	from	embedded	224:231	arg1	matrix					262:267	a hydrated macromolecular matrix	236:267	a hydrated macromolecular matrix which offers a number of advantages to the cells	236:316	Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells.					
32131450	2	56	theme	important	357:365	arg1	role					367:370	an important role	354:370	an important role	354:370	Extracellular polysaccharides play an important role in matrix setup and maintenance.					
32131450	5	57	theme	collected	852:860	arg1	data					862:865	All the collected data	844:865	All the collected data	844:865	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	5	58	theme	structural	1155:1164	arg1	motifs					1166:1171	local structural motifs	1149:1171	local structural motifs which could give hints about the polysaccharide insolubility	1149:1232	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	7	59	theme	molecular	1504:1512	arg1	simulations					1523:1533	molecular dynamics simulations	1504:1533	molecular dynamics simulations	1504:1533	In addition, the good correlation between NOE data and calculated interatomic distances by molecular dynamics simulations validated potential energy functions used for calculations.					
32131450	7	60	theme	potential	1545:1553	arg1	functions					1562:1570	potential energy functions	1545:1570	potential energy functions used for calculations	1545:1592	In addition, the good correlation between NOE data and calculated interatomic distances by molecular dynamics simulations validated potential energy functions used for calculations.					
32131450	7	61	theme	energy	1555:1560	arg1	functions					1562:1570	potential energy functions	1545:1570	potential energy functions used for calculations	1545:1592	In addition, the good correlation between NOE data and calculated interatomic distances by molecular dynamics simulations validated potential energy functions used for calculations.					
32131450	4	62	theme	NMR	770:772	arg1	spectroscopy					774:785	NMR spectroscopy	770:785	NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions	770:841	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	0	63	from	Modelling	126:134	arg1	Biofilm					78:84	Biofilm	78:84	Biofilm	78:84	Burkholderia cenocepacia H111 Produces a Water-Insoluble Exopolysaccharide in Biofilm: Structural Determination and Molecular Modelling.					
32131450	5	64	theme	-α-d-Manp-	1020:1029	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	3	65	theme	cenocepacia	506:516	arg1	H111					525:528	Burkholderia cenocepacia strain H111	493:528	Burkholderia cenocepacia strain H111	493:528	A water-insoluble polysaccharide was isolated and purified from the biofilm produced by Burkholderia cenocepacia strain H111, a cystic fibrosis pathogen.					
32131450	4	66	from	spectroscopy	774:785	arg1	solutions					833:841	deuterated sodium hydroxide (NaOD) aqueous solutions	790:841	deuterated sodium hydroxide (NaOD) aqueous solutions	790:841	Its composition and glycosidic linkages were determined using Gas-Liquid Chromatography-Mass Spectrometry (GLC-MS) on appropriate carbohydrate derivatives while its complete structure was unraveled by 1D and 2D NMR spectroscopy in deuterated sodium hydroxide (NaOD) aqueous solutions.					
32131450	6	67	theme	conformations	1303:1315	arg1	dynamics					1285:1292	restricted dynamics	1274:1292	restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions	1274:1410	Both modelling and NMR data pointed at restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions.					
32131450	5	68	theme	-α-d-Galp-	975:984	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	1	69	theme	multicellular	152:164	arg1	Biofilms					137:144	Biofilms	137:144	Biofilms	137:144	Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells.					
32131450	1	69	theme	multicellular	152:164	arg1	way					166:168	a multicellular way	150:168	a multicellular way	150:168	Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells.					
32131450	6	70	theme	bonds	1379:1383	arg1	presence					1344:1351	the presence	1340:1351	the presence of inter-residue hydrogen bonds	1340:1383	Both modelling and NMR data pointed at restricted dynamics of local conformations which were ascribed to the presence of inter-residue hydrogen bonds and to steric restrictions.					
32131450	5	71	theme	repeating	894:902	arg1	unit					904:907	the following repeating unit	880:907	the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility	880:1232	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	3	72	theme	fibrosis	540:547	arg1	biofilm					473:479	the biofilm	469:479	the biofilm produced by Burkholderia cenocepacia strain H111	469:528	A water-insoluble polysaccharide was isolated and purified from the biofilm produced by Burkholderia cenocepacia strain H111, a cystic fibrosis pathogen.					
32131450	3	72	theme	fibrosis	540:547	arg1	pathogen					549:556	a cystic fibrosis pathogen	531:556	a cystic fibrosis pathogen	531:556	A water-insoluble polysaccharide was isolated and purified from the biofilm produced by Burkholderia cenocepacia strain H111, a cystic fibrosis pathogen.					
32131450	5	73	theme	B.	933:934	arg1	modelling					1046:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling	913:1054	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	1	74	theme	hydrated	238:245	arg1	matrix					262:267	a hydrated macromolecular matrix	236:267	a hydrated macromolecular matrix which offers a number of advantages to the cells	236:316	Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells.					
32131450	5	75	theme	local	1149:1153	arg1	motifs					1166:1171	local structural motifs	1149:1171	local structural motifs which could give hints about the polysaccharide insolubility	1149:1232	All the collected data demonstrated the following repeating unit for the water-insoluble B. cenocepacia biofilm polysaccharide: [3)-α-d-Galp-(1→3)-α-d-Glcp-(1→3)-α-d-Galp-(1→3)-α-d-Manp-(1→]n Molecular modelling was used, coupled with NMR Nuclear Overhauser Effect (NOE) data, to obtain information about local structural motifs which could give hints about the polysaccharide insolubility.					
32131450	2	76	theme	matrix	375:380	arg1	setup					382:386	matrix setup	375:386	matrix setup	375:386	Extracellular polysaccharides play an important role in matrix setup and maintenance.					
32131450	1	77	theme	macromolecular	247:260	arg1	matrix					262:267	a hydrated macromolecular matrix	236:267	a hydrated macromolecular matrix which offers a number of advantages to the cells	236:316	Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells.					
32027895	5	0	theme	Western	761:767	arg1	analysis					774:781	Western blot analysis	761:781	Western blot analysis	761:781	Western blot analysis showed that the expression of proteins was related to apoptosis and autophagy.					
32027895	2	1	theme	polysaccharide	383:396	arg1	composition					329:339	composition	329:339	composition	329:339	The molecular weight, composition, and structure of the Polygala tenuifolia polysaccharide (PTP) was detected by HPLC, HPAEC-PAD, NMR and FT-IR.					
32027895	2	1	theme	polysaccharide	383:396	arg1	structure					346:354	structure	346:354	structure	346:354	The molecular weight, composition, and structure of the Polygala tenuifolia polysaccharide (PTP) was detected by HPLC, HPAEC-PAD, NMR and FT-IR.					
32027895	2	1	theme	polysaccharide	383:396	arg1	weight					321:326	The molecular weight	307:326	The molecular weight	307:326	The molecular weight, composition, and structure of the Polygala tenuifolia polysaccharide (PTP) was detected by HPLC, HPAEC-PAD, NMR and FT-IR.					
32027895	1	2	theme	FAS/FAS-L-mediated	265:282	arg1	pathway					284:290	the FAS/FAS-L-mediated pathway	261:290	the FAS/FAS-L-mediated pathway	261:290	In this study, a water-soluble polysaccharide was extracted from Polygonum tenuifolia to inhibit the proliferation of SPC-A-1 cells by the FAS/FAS-L-mediated pathway and autophagy.					
32027895	5	3	theme	blot	769:772	arg1	analysis					774:781	Western blot analysis	761:781	Western blot analysis	761:781	Western blot analysis showed that the expression of proteins was related to apoptosis and autophagy.					
32027895	7	4	theme	P62	1102:1104	arg1	down-regulation					1083:1097	the down-regulation	1079:1097	the down-regulation of P62	1079:1104	The up-regulation of LC 3B-II and the down-regulation of P62 indicated the occurrence of autophagy.					
32027895	7	4	theme	P62	1102:1104	arg1	up-regulation					1049:1061	The up-regulation	1045:1061	The up-regulation of LC 3B-II	1045:1073	The up-regulation of LC 3B-II and the down-regulation of P62 indicated the occurrence of autophagy.					
32027895	2	5	theme	tenuifolia	372:381	arg1	polysaccharide					383:396	the Polygala tenuifolia polysaccharide	359:396	the Polygala tenuifolia polysaccharide (PTP)	359:402	The molecular weight, composition, and structure of the Polygala tenuifolia polysaccharide (PTP) was detected by HPLC, HPAEC-PAD, NMR and FT-IR.					
32027895	2	5	theme	tenuifolia	372:381	arg1	PTP					399:401	PTP	399:401	PTP	399:401	The molecular weight, composition, and structure of the Polygala tenuifolia polysaccharide (PTP) was detected by HPLC, HPAEC-PAD, NMR and FT-IR.					
32027895	6	6	theme	up-regulated	906:917	arg1	expression					919:928	the up-regulated expression	902:928	the up-regulated expression	902:928	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
32027895	4	7	theme	Morphological	571:583	arg1	changes					585:591	Morphological changes	571:591	Morphological changes	571:591	Morphological changes were observed with microscopes, and the cell apoptosis-related markers detected by flow cytometry indicated that apoptosis and autophagy occurred in the SPC-A-1 cells.					
32027895	3	8	with	Glc	510:512	arg1	β-configurations					553:568	β-configurations	553:568	β-configurations	553:568	The purified polysaccharide was composed of Ara, Gal, and Glc (molar ratio: 2.6:1.8:1.0) with α- and β-configurations.					
32027895	3	8	with	Glc	510:512	arg1	α-					546:547	α-	546:547	α-	546:547	The purified polysaccharide was composed of Ara, Gal, and Glc (molar ratio: 2.6:1.8:1.0) with α- and β-configurations.					
32027895	2	9	theme	molecular	311:319	arg1	weight					321:326	The molecular weight	307:326	The molecular weight	307:326	The molecular weight, composition, and structure of the Polygala tenuifolia polysaccharide (PTP) was detected by HPLC, HPAEC-PAD, NMR and FT-IR.					
32027895	7	10	theme	3B-II	1069:1073	arg1	down-regulation					1083:1097	the down-regulation	1079:1097	the down-regulation of P62	1079:1104	The up-regulation of LC 3B-II and the down-regulation of P62 indicated the occurrence of autophagy.					
32027895	7	10	theme	3B-II	1069:1073	arg1	up-regulation					1049:1061	The up-regulation	1045:1061	The up-regulation of LC 3B-II	1045:1073	The up-regulation of LC 3B-II and the down-regulation of P62 indicated the occurrence of autophagy.					
32027895	4	11	theme	apoptosis-related	638:654	arg1	markers					656:662	the cell apoptosis-related markers	629:662	the cell apoptosis-related markers detected by flow cytometry	629:689	Morphological changes were observed with microscopes, and the cell apoptosis-related markers detected by flow cytometry indicated that apoptosis and autophagy occurred in the SPC-A-1 cells.					
32027895	6	12	theme	cascade	977:983	arg1	reaction					985:992	a cascade reaction	975:992	a cascade reaction of the caspase family that induced cell apoptosis	975:1042	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
32027895	3	13	theme	molar	515:519	arg1	ratio					521:525	molar ratio	515:525	molar ratio	515:525	The purified polysaccharide was composed of Ara, Gal, and Glc (molar ratio: 2.6:1.8:1.0) with α- and β-configurations.					
32027895	3	13	theme	molar	515:519	arg1	Gal					501:503	Gal	501:503	Gal	501:503	The purified polysaccharide was composed of Ara, Gal, and Glc (molar ratio: 2.6:1.8:1.0) with α- and β-configurations.					
32027895	4	14	theme	cell	633:636	arg1	markers					656:662	the cell apoptosis-related markers	629:662	the cell apoptosis-related markers detected by flow cytometry	629:689	Morphological changes were observed with microscopes, and the cell apoptosis-related markers detected by flow cytometry indicated that apoptosis and autophagy occurred in the SPC-A-1 cells.					
32027895	6	15	theme	receptor	872:879	arg1	pathway					881:887	The death receptor pathway	862:887	The death receptor pathway	862:887	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
32027895	8	16	theme	PTP	1347:1349	arg1	prevention					1308:1317	tumor prevention	1302:1317	tumor prevention	1302:1317	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	8	16	theme	PTP	1347:1349	arg1	application					1332:1342	clinical application	1323:1342	clinical application of PTP in the future	1323:1363	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	0	17	theme	FAS/FAS-L-mediated	0:17	arg1	apoptosis					19:27	FAS/FAS-L-mediated apoptosis	0:27	FAS/FAS-L-mediated apoptosis	0:27	FAS/FAS-L-mediated apoptosis and autophagy of SPC-A-1 cells induced by water-soluble polysaccharide from Polygala tenuifolia.					
32027895	7	18	theme	LC	1066:1067	arg1	3B-II					1069:1073	LC 3B-II	1066:1073	LC 3B-II	1066:1073	The up-regulation of LC 3B-II and the down-regulation of P62 indicated the occurrence of autophagy.					
32027895	5	19	theme	proteins	813:820	arg1	related					826:832	related	826:832	related	826:832	Western blot analysis showed that the expression of proteins was related to apoptosis and autophagy.					
32027895	5	19	theme	proteins	813:820	arg1	expression					799:808	the expression	795:808	the expression of proteins	795:820	Western blot analysis showed that the expression of proteins was related to apoptosis and autophagy.					
32027895	8	20	theme	strong	1273:1278	arg1	basis					1292:1296	a strong theoretical basis	1271:1296	a strong theoretical basis for tumor prevention and clinical application of PTP in the future	1271:1363	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	4	21	theme	SPC-A-1	746:752	arg1	cells					754:758	the SPC-A-1 cells	742:758	the SPC-A-1 cells	742:758	Morphological changes were observed with microscopes, and the cell apoptosis-related markers detected by flow cytometry indicated that apoptosis and autophagy occurred in the SPC-A-1 cells.					
32027895	6	22	theme	cell	1029:1032	arg1	apoptosis					1034:1042	cell apoptosis	1029:1042	cell apoptosis	1029:1042	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
32027895	6	23	theme	FADD	956:959	arg1	expression					919:928	the up-regulated expression	902:928	the up-regulated expression	902:928	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
32027895	6	24	theme	death	866:870	arg1	pathway					881:887	The death receptor pathway	862:887	The death receptor pathway	862:887	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
32027895	1	25	theme	water-soluble	143:155	arg1	polysaccharide					157:170	a water-soluble polysaccharide	141:170	a water-soluble polysaccharide	141:170	In this study, a water-soluble polysaccharide was extracted from Polygonum tenuifolia to inhibit the proliferation of SPC-A-1 cells by the FAS/FAS-L-mediated pathway and autophagy.					
32027895	0	26	theme	SPC-A-1	46:52	arg1	cells					54:58	SPC-A-1 cells	46:58	SPC-A-1 cells	46:58	FAS/FAS-L-mediated apoptosis and autophagy of SPC-A-1 cells induced by water-soluble polysaccharide from Polygala tenuifolia.					
32027895	8	27	from	prevention	1308:1317	arg1	future					1358:1363	future	1358:1363	future	1358:1363	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	7	28	theme	autophagy	1134:1142	arg1	occurrence					1120:1129	the occurrence	1116:1129	the occurrence of autophagy	1116:1142	The up-regulation of LC 3B-II and the down-regulation of P62 indicated the occurrence of autophagy.					
32027895	8	29	from	autophagy	1231:1239	arg1	cells					1252:1256	SPC-A-1 cells	1244:1256	SPC-A-1 cells	1244:1256	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	3	30	theme	purified	456:463	arg1	polysaccharide					465:478	The purified polysaccharide	452:478	The purified polysaccharide	452:478	The purified polysaccharide was composed of Ara, Gal, and Glc (molar ratio: 2.6:1.8:1.0) with α- and β-configurations.					
32027895	6	31	theme	FAS-L	945:949	arg1	expression					919:928	the up-regulated expression	902:928	the up-regulated expression	902:928	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
32027895	2	32	theme	Polygala	363:370	arg1	polysaccharide					383:396	the Polygala tenuifolia polysaccharide	359:396	the Polygala tenuifolia polysaccharide (PTP)	359:402	The molecular weight, composition, and structure of the Polygala tenuifolia polysaccharide (PTP) was detected by HPLC, HPAEC-PAD, NMR and FT-IR.					
32027895	2	32	theme	Polygala	363:370	arg1	PTP					399:401	PTP	399:401	PTP	399:401	The molecular weight, composition, and structure of the Polygala tenuifolia polysaccharide (PTP) was detected by HPLC, HPAEC-PAD, NMR and FT-IR.					
32027895	0	33	theme	cells	54:58	arg1	autophagy					33:41	autophagy	33:41	autophagy	33:41	FAS/FAS-L-mediated apoptosis and autophagy of SPC-A-1 cells induced by water-soluble polysaccharide from Polygala tenuifolia.					
32027895	0	33	theme	cells	54:58	arg1	apoptosis					19:27	FAS/FAS-L-mediated apoptosis	0:27	FAS/FAS-L-mediated apoptosis	0:27	FAS/FAS-L-mediated apoptosis and autophagy of SPC-A-1 cells induced by water-soluble polysaccharide from Polygala tenuifolia.					
32027895	6	34	theme	ligand	938:943	arg1	FAS-L					945:949	ligand FAS-L	938:949	ligand FAS-L	938:949	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
32027895	8	35	from	apoptosis	1217:1225	arg1	cells					1252:1256	SPC-A-1 cells	1244:1256	SPC-A-1 cells	1244:1256	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	8	36	from	application	1332:1342	arg1	future					1358:1363	future	1358:1363	future	1358:1363	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	3	37	dep	ratio	521:525	arg1	2.6:1.8:1.0					528:538	2.6:1.8:1.0	528:538	2.6:1.8:1.0	528:538	The purified polysaccharide was composed of Ara, Gal, and Glc (molar ratio: 2.6:1.8:1.0) with α- and β-configurations.					
32027895	1	38	theme	SPC-A-1	244:250	arg1	cells					252:256	SPC-A-1 cells	244:256	SPC-A-1 cells	244:256	In this study, a water-soluble polysaccharide was extracted from Polygonum tenuifolia to inhibit the proliferation of SPC-A-1 cells by the FAS/FAS-L-mediated pathway and autophagy.					
32027895	8	39	theme	clinical	1323:1330	arg1	application					1332:1342	clinical application	1323:1342	clinical application of PTP in the future	1323:1363	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	0	40	from	tenuifolia	114:123	arg1	polysaccharide					85:98	water-soluble polysaccharide	71:98	water-soluble polysaccharide from Polygala tenuifolia	71:123	FAS/FAS-L-mediated apoptosis and autophagy of SPC-A-1 cells induced by water-soluble polysaccharide from Polygala tenuifolia.					
32027895	8	41	theme	theoretical	1280:1290	arg1	basis					1292:1296	a strong theoretical basis	1271:1296	a strong theoretical basis for tumor prevention and clinical application of PTP in the future	1271:1363	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	8	42	theme	tumor	1302:1306	arg1	prevention					1308:1317	tumor prevention	1302:1317	tumor prevention	1302:1317	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	1	43	theme	cells	252:256	arg1	proliferation					227:239	the proliferation	223:239	the proliferation of SPC-A-1 cells	223:256	In this study, a water-soluble polysaccharide was extracted from Polygonum tenuifolia to inhibit the proliferation of SPC-A-1 cells by the FAS/FAS-L-mediated pathway and autophagy.					
32027895	0	44	theme	water-soluble	71:83	arg1	polysaccharide					85:98	water-soluble polysaccharide	71:98	water-soluble polysaccharide from Polygala tenuifolia	71:123	FAS/FAS-L-mediated apoptosis and autophagy of SPC-A-1 cells induced by water-soluble polysaccharide from Polygala tenuifolia.					
32027895	6	45	theme	family	1009:1014	arg1	reaction					985:992	a cascade reaction	975:992	a cascade reaction of the caspase family that induced cell apoptosis	975:1042	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
32027895	8	46	theme	SPC-A-1	1244:1250	arg1	cells					1252:1256	SPC-A-1 cells	1244:1256	SPC-A-1 cells	1244:1256	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	4	47	theme	flow	676:679	arg1	cytometry					681:689	flow cytometry	676:689	flow cytometry	676:689	Morphological changes were observed with microscopes, and the cell apoptosis-related markers detected by flow cytometry indicated that apoptosis and autophagy occurred in the SPC-A-1 cells.					
32027895	8	48	theme	FAS/FAS-L-mediated	1198:1215	arg1	apoptosis					1217:1225	FAS/FAS-L-mediated apoptosis	1198:1225	FAS/FAS-L-mediated apoptosis	1198:1225	In summary, these results showed that PTP can induce FAS/FAS-L-mediated apoptosis and autophagy in SPC-A-1 cells, and provide a strong theoretical basis for tumor prevention and clinical application of PTP in the future.					
32027895	6	49	theme	FAS	933:935	arg1	expression					919:928	the up-regulated expression	902:928	the up-regulated expression	902:928	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
32027895	6	50	theme	caspase	1001:1007	arg1	family					1009:1014	the caspase family	997:1014	the caspase family that induced cell apoptosis	997:1042	The death receptor pathway demonstrated the up-regulated expression of FAS, ligand FAS-L, and FADD, which led to a cascade reaction of the caspase family that induced cell apoptosis.					
33254056	12	0	dep	Pinus	1806:1810	arg1	massoniana					1812:1821	massoniana	1812:1821	massoniana	1812:1821	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	11	1	theme	factors	1613:1619	arg1	combination					1568:1578	a combination	1566:1578	a combination of multiple molecular structural factors	1566:1619	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	1	2	theme	activity	274:281	arg1	relationship					283:294	their structure activity relationship	258:294	their structure activity relationship	258:294	The objective was to identify the active fractions of polysaccharide against replication of ALV-J and elucidate their structure activity relationship.					
33254056	11	3	theme	multiple	1583:1590	arg1	factors					1613:1619	multiple molecular structural factors	1583:1619	multiple molecular structural factors	1583:1619	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	5	4	theme	molecular	640:648	arg1	weight					650:655	molecular weight	640:655	molecular weight of 463.70, 99.41, and 26.97 kDa	640:687	Three fractions, PPP-1, PPP-2, and PPP-3, were identified with molecular weight of 463.70, 99.41, and 26.97 kDa, respectively.					
33254056	9	5	theme	significant	1116:1126	arg1	cytotoxicity					1128:1139	no significant cytotoxicity	1113:1139	no significant cytotoxicity	1113:1139	In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05).					
33254056	9	6	theme	μg	1207:1208	arg1	mL-1					1210:1213	800 μg mL-1	1203:1213	800 μg mL-1 (P > 0.05)	1203:1224	In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05).					
33254056	9	6	theme	μg	1207:1208	arg1	>					1218:1218	P > 0.05	1216:1223	P > 0.05	1216:1223	In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05).					
33254056	0	7	theme	structure	99:107	arg1	relationship					118:129	their structure activity relationship	93:129	their structure activity relationship investigation	93:143	Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation.					
33254056	11	8	theme	molecular	1592:1600	arg1	factors					1613:1619	multiple molecular structural factors	1583:1619	multiple molecular structural factors	1583:1619	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	7	9	theme	galacturonic	940:951	arg1	acid					953:956	galacturonic acid	940:956	galacturonic acid	940:956	Compared with PPP-1, which was mainly composed of glucose, PPP-2 and PPP-3 contained a higher proportion of galactose, glucuronic acid and galacturonic acid.					
33254056	0	10	dep	relationship	118:129	arg1	investigation					131:143	investigation	131:143	their structure activity relationship investigation	93:143	Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation.					
33254056	11	11	theme	monosaccharide	1468:1481	arg1	structure					1533:1541	triple helical structure	1518:1541	triple helical structure	1518:1541	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	11	theme	monosaccharide	1468:1481	arg1	result					1556:1561	a result	1554:1561	a result of a combination of multiple molecular structural factors	1554:1619	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	11	theme	monosaccharide	1468:1481	arg1	composition					1483:1493	their monosaccharide composition	1462:1493	their monosaccharide composition	1462:1493	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	11	theme	monosaccharide	1468:1481	arg1	weight					1506:1511	molecular weight	1496:1511	molecular weight	1496:1511	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	12	theme	combination	1568:1578	arg1	structure					1533:1541	triple helical structure	1518:1541	triple helical structure	1518:1541	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	12	theme	combination	1568:1578	arg1	result					1556:1561	a result	1554:1561	a result of a combination of multiple molecular structural factors	1554:1619	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	12	theme	combination	1568:1578	arg1	composition					1483:1493	their monosaccharide composition	1462:1493	their monosaccharide composition	1462:1493	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	12	theme	combination	1568:1578	arg1	weight					1506:1511	molecular weight	1496:1511	molecular weight	1496:1511	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	12	13	theme	future	1763:1768	arg1	applications					1770:1781	future applications	1763:1781	future applications of polysaccharide from Pinus massoniana pollen	1763:1828	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	7	14	theme	glucuronic	920:929	arg1	acid					931:934	glucuronic acid	920:934	glucuronic acid	920:934	Compared with PPP-1, which was mainly composed of glucose, PPP-2 and PPP-3 contained a higher proportion of galactose, glucuronic acid and galacturonic acid.					
33254056	11	15	theme	structure-activity	1348:1365	arg1	relationship					1367:1378	The structure-activity relationship	1344:1378	The structure-activity relationship	1344:1378	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	8	16	theme	triple	1015:1020	arg1	structure					1030:1038	a triple helical structure	1013:1038	a triple helical structure	1013:1038	The Congo red assay indicated that the PPP-2 may have a triple helical structure, while PPP-1 and PPP-3 were absent.					
33254056	6	17	theme	different	778:786	arg1	proportions					788:798	different proportions	778:798	different proportions	778:798	Three polysaccharide fractions were all composed of 10 monosaccharides in different proportions.					
33254056	2	18	theme	extraction	309:318	arg1	conditions					320:329	The optimal extraction conditions	297:329	The optimal extraction conditions	297:329	The optimal extraction conditions were extracting temperature 90℃, pH 9 and the ratio of liquid to solid 30:1.					
33254056	1	19	theme	active	180:185	arg1	fractions					187:195	the active fractions	176:195	the active fractions of polysaccharide against replication of ALV-J	176:242	The objective was to identify the active fractions of polysaccharide against replication of ALV-J and elucidate their structure activity relationship.					
33254056	0	20	theme	activity	109:116	arg1	relationship					118:129	their structure activity relationship	93:129	their structure activity relationship investigation	93:143	Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation.					
33254056	0	21	from	massoniana	38:47	arg1	fractions					17:25	Screening active fractions	0:25	Screening active fractions from Pinus massoniana	0:47	Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation.					
33254056	7	22	theme	galactose	909:917	arg1	proportion					895:904	a higher proportion	886:904	a higher proportion of galactose, glucuronic acid and galacturonic acid	886:956	Compared with PPP-1, which was mainly composed of glucose, PPP-2 and PPP-3 contained a higher proportion of galactose, glucuronic acid and galacturonic acid.					
33254056	2	23	theme	optimal	301:307	arg1	conditions					320:329	The optimal extraction conditions	297:329	The optimal extraction conditions	297:329	The optimal extraction conditions were extracting temperature 90℃, pH 9 and the ratio of liquid to solid 30:1.					
33254056	9	24	dep	In	1076:1077	arg1	vitro					1079:1083	vitro	1079:1083	vitro	1079:1083	In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05).					
33254056	3	25	theme	polysaccharide	458:471	arg1	yield					443:447	extraction yield	432:447	extraction yield of total polysaccharide	432:471	Under these conditions, extraction yield of total polysaccharide was 6.5 % ± 0.19 %.					
33254056	0	26	theme	active	10:15	arg1	fractions					17:25	Screening active fractions	0:25	Screening active fractions from Pinus massoniana	0:47	Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation.					
33254056	8	27	theme	red	969:971	arg1	assay					973:977	The Congo red assay	959:977	The Congo red assay	959:977	The Congo red assay indicated that the PPP-2 may have a triple helical structure, while PPP-1 and PPP-3 were absent.					
33254056	0	28	theme	Screening	0:8	arg1	fractions					17:25	Screening active fractions	0:25	Screening active fractions from Pinus massoniana	0:47	Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation.					
33254056	4	29	theme	G-200	566:570	arg1	gel					572:574	Sephadex G-200 gel	557:574	Sephadex G-200 gel	557:574	Total polysaccharide was then purified by DEAE-52 cellulose and Sephadex G-200 gel.					
33254056	8	30	theme	helical	1022:1028	arg1	structure					1030:1038	a triple helical structure	1013:1038	a triple helical structure	1013:1038	The Congo red assay indicated that the PPP-2 may have a triple helical structure, while PPP-1 and PPP-3 were absent.					
33254056	11	31	theme	triple	1518:1523	arg1	composition					1483:1493	their monosaccharide composition	1462:1493	their monosaccharide composition	1462:1493	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	31	theme	triple	1518:1523	arg1	result					1556:1561	a result	1554:1561	a result of a combination of multiple molecular structural factors	1554:1619	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	31	theme	triple	1518:1523	arg1	structure					1533:1541	triple helical structure	1518:1541	triple helical structure	1518:1541	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	31	theme	triple	1518:1523	arg1	weight					1506:1511	molecular weight	1496:1511	molecular weight	1496:1511	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	12	32	theme	antiviral	1853:1861	arg1	agent					1863:1867	antiviral agent	1853:1867	antiviral agent	1853:1867	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	2	33	theme	solid	396:400	arg1	30:1					402:405	solid 30:1	396:405	solid 30:1	396:405	The optimal extraction conditions were extracting temperature 90℃, pH 9 and the ratio of liquid to solid 30:1.					
33254056	12	34	theme	synthetic	1711:1719	arg1	drugs					1731:1735	synthetic antiviral drugs	1711:1735	synthetic antiviral drugs	1711:1735	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	4	35	theme	Sephadex	557:564	arg1	gel					572:574	Sephadex G-200 gel	557:574	Sephadex G-200 gel	557:574	Total polysaccharide was then purified by DEAE-52 cellulose and Sephadex G-200 gel.					
33254056	7	36	theme	acid	931:934	arg1	proportion					895:904	a higher proportion	886:904	a higher proportion of galactose, glucuronic acid and galacturonic acid	886:956	Compared with PPP-1, which was mainly composed of glucose, PPP-2 and PPP-3 contained a higher proportion of galactose, glucuronic acid and galacturonic acid.					
33254056	10	37	theme	antiviral	1323:1331	arg1	component					1333:1341	the major antiviral component	1313:1341	the major antiviral component	1313:1341	The antiviral test showed that PPP-2 had the strongest activity, indicating PPP-2 was the major antiviral component.					
33254056	10	37	theme	antiviral	1323:1331	arg1	PPP-2					1303:1307	PPP-2	1303:1307	PPP-2	1303:1307	The antiviral test showed that PPP-2 had the strongest activity, indicating PPP-2 was the major antiviral component.					
33254056	10	38	theme	antiviral	1231:1239	arg1	test					1241:1244	The antiviral test	1227:1244	The antiviral test	1227:1244	The antiviral test showed that PPP-2 had the strongest activity, indicating PPP-2 was the major antiviral component.					
33254056	11	39	theme	antiviral	1396:1404	arg1	activities					1406:1415	the antiviral activities	1392:1415	the antiviral activities of polysaccharide fractions	1392:1443	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	8	40	contain	have	1008:1011	arg2	structure					1030:1038	a triple helical structure	1013:1038	a triple helical structure	1013:1038	The Congo red assay indicated that the PPP-2 may have a triple helical structure, while PPP-1 and PPP-3 were absent.					
33254056	8	40	contain	have	1008:1011	arg1	PPP-2					998:1002	the PPP-2	994:1002	the PPP-2	994:1002	The Congo red assay indicated that the PPP-2 may have a triple helical structure, while PPP-1 and PPP-3 were absent.					
33254056	5	41	theme	kDa	685:687	arg1	weight					650:655	molecular weight	640:655	molecular weight of 463.70, 99.41, and 26.97 kDa	640:687	Three fractions, PPP-1, PPP-2, and PPP-3, were identified with molecular weight of 463.70, 99.41, and 26.97 kDa, respectively.					
33254056	1	42	theme	polysaccharide	200:213	arg1	fractions					187:195	the active fractions	176:195	the active fractions of polysaccharide against replication of ALV-J	176:242	The objective was to identify the active fractions of polysaccharide against replication of ALV-J and elucidate their structure activity relationship.					
33254056	3	43	theme	extraction	432:441	arg1	yield					443:447	extraction yield	432:447	extraction yield of total polysaccharide	432:471	Under these conditions, extraction yield of total polysaccharide was 6.5 % ± 0.19 %.					
33254056	4	44	theme	DEAE-52	535:541	arg1	cellulose					543:551	DEAE-52 cellulose	535:551	DEAE-52 cellulose	535:551	Total polysaccharide was then purified by DEAE-52 cellulose and Sephadex G-200 gel.					
33254056	12	45	theme	useful	1835:1840	arg1	support					1751:1757	support	1751:1757	support for future applications of polysaccharide from Pinus massoniana pollen	1751:1828	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	12	45	theme	useful	1835:1840	arg1	source					1842:1847	a useful source	1833:1847	a useful source for antiviral agent	1833:1867	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	9	46	theme	In	1076:1077	arg1	assay					1085:1089	In vitro assay	1076:1089	In vitro assay	1076:1089	In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05).					
33254056	1	47	theme	ALV-J	238:242	arg1	replication					223:233	replication	223:233	replication of ALV-J	223:242	The objective was to identify the active fractions of polysaccharide against replication of ALV-J and elucidate their structure activity relationship.					
33254056	12	48	from	pollen	1823:1828	arg1	applications					1770:1781	future applications	1763:1781	future applications of polysaccharide from Pinus massoniana pollen	1763:1828	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	12	48	from	pollen	1823:1828	arg1	polysaccharide					1786:1799	polysaccharide	1786:1799	polysaccharide from Pinus massoniana pollen	1786:1828	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	12	49	theme	Pinus	1806:1810	arg1	pollen					1823:1828	Pinus massoniana pollen	1806:1828	Pinus massoniana pollen	1806:1828	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	3	50	theme	total	452:456	arg1	polysaccharide					458:471	total polysaccharide	452:471	total polysaccharide	452:471	Under these conditions, extraction yield of total polysaccharide was 6.5 % ± 0.19 %.					
33254056	4	51	theme	Total	493:497	arg1	polysaccharide					499:512	Total polysaccharide	493:512	Total polysaccharide	493:512	Total polysaccharide was then purified by DEAE-52 cellulose and Sephadex G-200 gel.					
33254056	12	52	theme	antiviral	1721:1729	arg1	drugs					1731:1735	synthetic antiviral drugs	1711:1735	synthetic antiviral drugs	1711:1735	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	12	53	theme	valued	1682:1687	arg1	PPP-2					1652:1656	the PPP-2	1648:1656	the PPP-2	1648:1656	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	12	53	theme	valued	1682:1687	arg1	support					1751:1757	support	1751:1757	support for future applications of polysaccharide from Pinus massoniana pollen	1751:1828	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	12	53	theme	valued	1682:1687	arg1	product					1689:1695	a valued product	1680:1695	a valued product	1680:1695	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	9	54	theme	mL-1	1210:1213	arg1	concentration					1186:1198	the concentration	1182:1198	the concentration of 800 μg mL-1 (P > 0.05)	1182:1224	In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05).					
33254056	12	55	theme	polysaccharide	1786:1799	arg1	applications					1770:1781	future applications	1763:1781	future applications of polysaccharide from Pinus massoniana pollen	1763:1828	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	10	56	contain	had	1264:1266	arg1	PPP-2					1258:1262	PPP-2	1258:1262	PPP-2	1258:1262	The antiviral test showed that PPP-2 had the strongest activity, indicating PPP-2 was the major antiviral component.					
33254056	10	56	contain	had	1264:1266	arg2	activity					1282:1289	the strongest activity	1268:1289	the strongest activity	1268:1289	The antiviral test showed that PPP-2 had the strongest activity, indicating PPP-2 was the major antiviral component.					
33254056	11	57	theme	helical	1525:1531	arg1	composition					1483:1493	their monosaccharide composition	1462:1493	their monosaccharide composition	1462:1493	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	57	theme	helical	1525:1531	arg1	result					1556:1561	a result	1554:1561	a result of a combination of multiple molecular structural factors	1554:1619	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	57	theme	helical	1525:1531	arg1	structure					1533:1541	triple helical structure	1518:1541	triple helical structure	1518:1541	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	57	theme	helical	1525:1531	arg1	weight					1506:1511	molecular weight	1496:1511	molecular weight	1496:1511	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	9	58	theme	polysaccharide	1151:1164	arg1	fractions					1166:1174	the polysaccharide fractions	1147:1174	the polysaccharide fractions	1147:1174	In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05).					
33254056	11	59	theme	molecular	1496:1504	arg1	structure					1533:1541	triple helical structure	1518:1541	triple helical structure	1518:1541	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	59	theme	molecular	1496:1504	arg1	result					1556:1561	a result	1554:1561	a result of a combination of multiple molecular structural factors	1554:1619	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	59	theme	molecular	1496:1504	arg1	composition					1483:1493	their monosaccharide composition	1462:1493	their monosaccharide composition	1462:1493	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	59	theme	molecular	1496:1504	arg1	weight					1506:1511	molecular weight	1496:1511	molecular weight	1496:1511	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	10	60	theme	major	1317:1321	arg1	component					1333:1341	the major antiviral component	1313:1341	the major antiviral component	1313:1341	The antiviral test showed that PPP-2 had the strongest activity, indicating PPP-2 was the major antiviral component.					
33254056	10	60	theme	major	1317:1321	arg1	PPP-2					1303:1307	PPP-2	1303:1307	PPP-2	1303:1307	The antiviral test showed that PPP-2 had the strongest activity, indicating PPP-2 was the major antiviral component.					
33254056	12	61	from	applications	1770:1781	arg1	pollen					1823:1828	Pinus massoniana pollen	1806:1828	Pinus massoniana pollen	1806:1828	These results showed that the PPP-2 could be exploited as a valued product for replacing synthetic antiviral drugs, and provided support for future applications of polysaccharide from Pinus massoniana pollen as a useful source for antiviral agent.					
33254056	7	62	theme	higher	888:893	arg1	proportion					895:904	a higher proportion	886:904	a higher proportion of galactose, glucuronic acid and galacturonic acid	886:956	Compared with PPP-1, which was mainly composed of glucose, PPP-2 and PPP-3 contained a higher proportion of galactose, glucuronic acid and galacturonic acid.					
33254056	9	63	theme	P	1216:1216	arg1	mL-1					1210:1213	800 μg mL-1	1203:1213	800 μg mL-1 (P > 0.05)	1203:1224	In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05).					
33254056	9	63	theme	P	1216:1216	arg1	>					1218:1218	P > 0.05	1216:1223	P > 0.05	1216:1223	In vitro assay showed that there was no significant cytotoxicity among the polysaccharide fractions under the concentration of 800 μg mL-1 (P > 0.05).					
33254056	10	64	theme	strongest	1272:1280	arg1	activity					1282:1289	the strongest activity	1268:1289	the strongest activity	1268:1289	The antiviral test showed that PPP-2 had the strongest activity, indicating PPP-2 was the major antiviral component.					
33254056	6	65	theme	polysaccharide	710:723	arg1	fractions					725:733	Three polysaccharide fractions	704:733	Three polysaccharide fractions	704:733	Three polysaccharide fractions were all composed of 10 monosaccharides in different proportions.					
33254056	11	66	theme	polysaccharide	1420:1433	arg1	fractions					1435:1443	polysaccharide fractions	1420:1443	polysaccharide fractions	1420:1443	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	11	67	theme	fractions	1435:1443	arg1	activities					1406:1415	the antiviral activities	1392:1415	the antiviral activities of polysaccharide fractions	1392:1443	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	0	68	theme	ALV-J	71:75	arg1	replication					77:87	ALV-J replication	71:87	ALV-J replication	71:87	Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation.					
33254056	6	69	from	monosaccharides	759:773	arg1	proportions					788:798	different proportions	778:798	different proportions	778:798	Three polysaccharide fractions were all composed of 10 monosaccharides in different proportions.					
33254056	3	70	dep	%	481:481	arg1	%					490:490	± 0.19 %	483:490	6.5 % ± 0.19 %	477:490	Under these conditions, extraction yield of total polysaccharide was 6.5 % ± 0.19 %.					
33254056	11	71	theme	structural	1602:1611	arg1	factors					1613:1619	multiple molecular structural factors	1583:1619	multiple molecular structural factors	1583:1619	The structure-activity relationship showed that the antiviral activities of polysaccharide fractions were affected by their monosaccharide composition, molecular weight, and triple helical structure, which was a result of a combination of multiple molecular structural factors.					
33254056	7	72	theme	acid	953:956	arg1	proportion					895:904	a higher proportion	886:904	a higher proportion of galactose, glucuronic acid and galacturonic acid	886:956	Compared with PPP-1, which was mainly composed of glucose, PPP-2 and PPP-3 contained a higher proportion of galactose, glucuronic acid and galacturonic acid.					
33254056	2	73	theme	liquid	386:391	arg1	temperature					347:357	temperature 90℃	347:361	temperature 90℃	347:361	The optimal extraction conditions were extracting temperature 90℃, pH 9 and the ratio of liquid to solid 30:1.					
33254056	2	73	theme	liquid	386:391	arg1	pH					364:365	pH 9	364:367	pH 9	364:367	The optimal extraction conditions were extracting temperature 90℃, pH 9 and the ratio of liquid to solid 30:1.					
33254056	2	73	theme	liquid	386:391	arg1	ratio					377:381	the ratio	373:381	the ratio of liquid to solid 30:1	373:405	The optimal extraction conditions were extracting temperature 90℃, pH 9 and the ratio of liquid to solid 30:1.					
33254056	1	74	theme	structure	264:272	arg1	relationship					283:294	their structure activity relationship	258:294	their structure activity relationship	258:294	The objective was to identify the active fractions of polysaccharide against replication of ALV-J and elucidate their structure activity relationship.					
32041370	0	0	theme	Polysaccharides	91:105	arg1	Activity					72:79	Anticoagulant Activity	58:79	Anticoagulant Activity	58:79	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	0	0	theme	Polysaccharides	91:105	arg1	Optimization					11:22	Extraction Optimization	0:22	Extraction Optimization	0:22	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	0	0	theme	Polysaccharides	91:105	arg1	Characterization					36:51	Structural Characterization	25:51	Structural Characterization	25:51	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	2	1	theme	optimized	474:482	arg1	parameters					495:504	the optimized extraction parameters	470:504	the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W)	470:651	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	6	2	theme	time	1184:1187	arg1	results					1087:1093	the results	1083:1093	the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT)	1083:1192	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	1	3	theme	assisted	286:293	arg1	extraction					295:304	ultrasound assisted extraction	275:304	ultrasound assisted extraction (UAE)	275:310	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	1	3	theme	assisted	286:293	arg1	UAE					307:309	UAE	307:309	UAE	307:309	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	0	4	theme	Acidic	84:89	arg1	Polysaccharides					91:105	Acidic Polysaccharides	84:105	Acidic Polysaccharides from Auricularia auricula-judae	84:137	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	0	5	from	Optimization	11:22	arg1	auricula-judae					124:137	Auricularia auricula-judae	112:137	Auricularia auricula-judae	112:137	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	6	6	theme	time	1135:1138	arg1	results					1087:1093	the results	1083:1093	the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT)	1083:1192	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	6	7	theme	anticoagulant	1260:1272	arg1	activity					1274:1281	a synergic anticoagulant activity	1249:1281	a synergic anticoagulant activity	1249:1281	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	6	7	theme	anticoagulant	1260:1272	arg1	activity					1229:1236	anticoagulant activity	1215:1236	anticoagulant activity	1215:1236	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	4	8	theme	ion	804:806	arg1	chromatography					817:830	ion exchange chromatography	804:830	ion exchange chromatography with a DEAE Purose 6 Fast Flow column	804:868	aAAP was further purified using ion exchange chromatography with a DEAE Purose 6 Fast Flow column to obtain aAAP-1.					
32041370	0	9	theme	Auricularia	112:122	arg1	auricula-judae					124:137	Auricularia auricula-judae	112:137	Auricularia auricula-judae	112:137	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	1	10	theme	response	363:370	arg1	methodology					380:390	a response surface methodology	361:390	a response surface methodology (RSM)	361:396	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	1	10	theme	response	363:370	arg1	RSM					393:395	RSM	393:395	RSM	393:395	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	6	11	theme	thromboplastin	1120:1133	arg1	APTT					1141:1144	APTT	1141:1144	APTT	1141:1144	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	6	11	theme	thromboplastin	1120:1133	arg1	time					1135:1138	the activated partial thromboplastin time	1098:1138	the activated partial thromboplastin time (APTT)	1098:1145	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	2	12	theme	raw	599:601	arg1	material					603:610	raw material	599:610	raw material (103 mL/g)	599:621	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	12	theme	raw	599:601	arg1	mL/g					617:620	103 mL/g	613:620	103 mL/g	613:620	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	1	13	dep	Auricularia	151:161	arg1	auricula-judae					163:176	auricula-judae	163:176	auricula-judae	163:176	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	6	14	theme	synergic	1251:1258	arg1	activity					1274:1281	a synergic anticoagulant activity	1249:1281	a synergic anticoagulant activity	1249:1281	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	6	14	theme	synergic	1251:1258	arg1	activity					1229:1236	anticoagulant activity	1215:1236	anticoagulant activity	1215:1236	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	3	15	theme	cetyltrimethylammonium	711:732	arg1	bromide					734:740	cetyltrimethylammonium bromide	711:740	cetyltrimethylammonium bromide (CTAB)	711:747	Furthermore, the acidic AAP (aAAP) was precipitated with cetyltrimethylammonium bromide (CTAB) from crude AAP (cAAP).					
32041370	3	15	theme	cetyltrimethylammonium	711:732	arg1	CTAB					743:746	CTAB	743:746	CTAB	743:746	Furthermore, the acidic AAP (aAAP) was precipitated with cetyltrimethylammonium bromide (CTAB) from crude AAP (cAAP).					
32041370	6	16	theme	thrombin	1175:1182	arg1	TT					1190:1191	TT	1190:1191	TT	1190:1191	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	6	16	theme	thrombin	1175:1182	arg1	time					1184:1187	thrombin time	1175:1187	thrombin time (TT)	1175:1192	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	1	17	theme	surface	372:378	arg1	methodology					380:390	a response surface methodology	361:390	a response surface methodology (RSM)	361:396	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	1	17	theme	surface	372:378	arg1	RSM					393:395	RSM	393:395	RSM	393:395	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	6	18	theme	partial	1112:1118	arg1	APTT					1141:1144	APTT	1141:1144	APTT	1141:1144	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	6	18	theme	partial	1112:1118	arg1	time					1135:1138	the activated partial thromboplastin time	1098:1138	the activated partial thromboplastin time (APTT)	1098:1145	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	4	19	with	chromatography	817:830	arg1	column					863:868	a DEAE Purose 6 Fast Flow column	837:868	a DEAE Purose 6 Fast Flow column	837:868	aAAP was further purified using ion exchange chromatography with a DEAE Purose 6 Fast Flow column to obtain aAAP-1.					
32041370	5	20	theme	80.63:9.88:2.25:1:31.13	1048:1070	arg1	ratio					1039:1043	a molar ratio	1031:1043	a molar ratio of 80.63:9.88:2.25:1:31.13	1031:1070	Additionally, according to the HPLC analysis, the aAAP-1 was mainly composed of mannose, glucuronic acid, glucose, galactose, and xylose, with a molar ratio of 80.63:9.88:2.25:1:31.13.					
32041370	4	21	theme	Purose	844:849	arg1	column					863:868	a DEAE Purose 6 Fast Flow column	837:868	a DEAE Purose 6 Fast Flow column	837:868	aAAP was further purified using ion exchange chromatography with a DEAE Purose 6 Fast Flow column to obtain aAAP-1.					
32041370	6	22	theme	activated	1102:1110	arg1	APTT					1141:1144	APTT	1141:1144	APTT	1141:1144	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	6	22	theme	activated	1102:1110	arg1	time					1135:1138	the activated partial thromboplastin time	1098:1138	the activated partial thromboplastin time (APTT)	1098:1145	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	2	23	theme	liquid	589:594	arg1	min					570:572	27 min	567:572	27 min	567:572	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	23	theme	liquid	589:594	arg1	W					650:650	198 W	646:650	198 W	646:650	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	23	theme	liquid	589:594	arg1	time					561:564	extraction time	550:564	extraction time (27 min)	550:573	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	23	theme	liquid	589:594	arg1	temperature					529:539	Extraction temperature	518:539	Extraction temperature (74 °C)	518:547	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	23	theme	liquid	589:594	arg1	power					639:643	ultrasound power	628:643	ultrasound power (198 W)	628:651	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	23	theme	liquid	589:594	arg1	ratio					580:584	the ratio	576:584	the ratio of liquid to raw material (103 mL/g)	576:621	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	23	theme	liquid	589:594	arg1	°C					545:546	74 °C	542:546	74 °C	542:546	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	24	theme	maximum	403:409	arg1	yield					422:426	The maximum extraction yield	399:426	The maximum extraction yield of crude AAP (14.74 mg/g)	399:452	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	4	25	theme	DEAE	839:842	arg1	column					863:868	a DEAE Purose 6 Fast Flow column	837:868	a DEAE Purose 6 Fast Flow column	837:868	aAAP was further purified using ion exchange chromatography with a DEAE Purose 6 Fast Flow column to obtain aAAP-1.					
32041370	5	26	theme	molar	1033:1037	arg1	ratio					1039:1043	a molar ratio	1031:1043	a molar ratio of 80.63:9.88:2.25:1:31.13	1031:1070	Additionally, according to the HPLC analysis, the aAAP-1 was mainly composed of mannose, glucuronic acid, glucose, galactose, and xylose, with a molar ratio of 80.63:9.88:2.25:1:31.13.					
32041370	1	27	theme	natural	203:209	arg1	anticoagulants					211:224	natural anticoagulants	203:224	natural anticoagulants	203:224	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	1	27	theme	natural	203:209	arg1	polysaccharides					178:192	Auricularia auricula-judae polysaccharides	151:192	Auricularia auricula-judae polysaccharides (AAP)	151:198	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	0	28	from	Characterization	36:51	arg1	auricula-judae					124:137	Auricularia auricula-judae	112:137	Auricularia auricula-judae	112:137	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	6	29	dep	indicated	1194:1202	arg1	had					1211:1213	had	1211:1213	indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway	1194:1321	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	0	30	theme	Extraction	0:9	arg1	Optimization					11:22	Extraction Optimization	0:22	Extraction Optimization	0:22	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	0	31	theme	Structural	25:34	arg1	Characterization					36:51	Structural Characterization	25:51	Structural Characterization	25:51	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	2	32	dep	follows	509:515	arg1	min					570:572	27 min	567:572	27 min	567:572	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	32	dep	follows	509:515	arg1	W					650:650	198 W	646:650	198 W	646:650	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	32	dep	follows	509:515	arg1	time					561:564	extraction time	550:564	extraction time (27 min)	550:573	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	32	dep	follows	509:515	arg1	temperature					529:539	Extraction temperature	518:539	Extraction temperature (74 °C)	518:547	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	32	dep	follows	509:515	arg1	power					639:643	ultrasound power	628:643	ultrasound power (198 W)	628:651	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	32	dep	follows	509:515	arg1	ratio					580:584	the ratio	576:584	the ratio of liquid to raw material (103 mL/g)	576:621	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	32	dep	follows	509:515	arg1	°C					545:546	74 °C	542:546	74 °C	542:546	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	33	theme	crude	431:435	arg1	AAP					437:439	crude AAP	431:439	crude AAP (14.74 mg/g)	431:452	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	33	theme	crude	431:435	arg1	mg/g					448:451	14.74 mg/g	442:451	14.74 mg/g	442:451	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	34	theme	AAP	437:439	arg1	yield					422:426	The maximum extraction yield	399:426	The maximum extraction yield of crude AAP (14.74 mg/g)	399:452	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	6	35	theme	exogenous	1305:1313	arg1	pathway					1315:1321	the endogenous and exogenous pathway	1286:1321	the endogenous and exogenous pathway	1286:1321	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	6	36	theme	anticoagulant	1215:1227	arg1	activity					1274:1281	a synergic anticoagulant activity	1249:1281	a synergic anticoagulant activity	1249:1281	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	6	36	theme	anticoagulant	1215:1227	arg1	activity					1229:1236	anticoagulant activity	1215:1236	anticoagulant activity	1215:1236	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	4	37	theme	Flow	858:861	arg1	column					863:868	a DEAE Purose 6 Fast Flow column	837:868	a DEAE Purose 6 Fast Flow column	837:868	aAAP was further purified using ion exchange chromatography with a DEAE Purose 6 Fast Flow column to obtain aAAP-1.					
32041370	4	38	theme	exchange	808:815	arg1	chromatography					817:830	ion exchange chromatography	804:830	ion exchange chromatography with a DEAE Purose 6 Fast Flow column	804:868	aAAP was further purified using ion exchange chromatography with a DEAE Purose 6 Fast Flow column to obtain aAAP-1.					
32041370	1	39	theme	functional	249:258	arg1	industry					265:272	the functional food industry	245:272	the functional food industry	245:272	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	0	40	theme	Anticoagulant	58:70	arg1	Activity					72:79	Anticoagulant Activity	58:79	Anticoagulant Activity	58:79	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	2	41	theme	Extraction	518:527	arg1	temperature					529:539	Extraction temperature	518:539	Extraction temperature (74 °C)	518:547	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	41	theme	Extraction	518:527	arg1	°C					545:546	74 °C	542:546	74 °C	542:546	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	6	42	theme	prothrombin	1148:1158	arg1	PT					1166:1167	PT	1166:1167	PT	1166:1167	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	6	42	theme	prothrombin	1148:1158	arg1	time					1160:1163	prothrombin time	1148:1163	prothrombin time (PT)	1148:1168	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	0	43	from	Activity	72:79	arg1	auricula-judae					124:137	Auricularia auricula-judae	112:137	Auricularia auricula-judae	112:137	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	2	44	theme	ultrasound	628:637	arg1	power					639:643	ultrasound power	628:643	ultrasound power (198 W)	628:651	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	44	theme	ultrasound	628:637	arg1	W					650:650	198 W	646:650	198 W	646:650	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	6	45	theme	time	1160:1163	arg1	results					1087:1093	the results	1083:1093	the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT)	1083:1192	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	3	46	theme	acidic	671:676	arg1	AAP					678:680	the acidic AAP	667:680	the acidic AAP (aAAP)	667:687	Furthermore, the acidic AAP (aAAP) was precipitated with cetyltrimethylammonium bromide (CTAB) from crude AAP (cAAP).					
32041370	3	46	theme	acidic	671:676	arg1	aAAP					683:686	aAAP	683:686	aAAP	683:686	Furthermore, the acidic AAP (aAAP) was precipitated with cetyltrimethylammonium bromide (CTAB) from crude AAP (cAAP).					
32041370	2	47	theme	extraction	550:559	arg1	min					570:572	27 min	567:572	27 min	567:572	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	47	theme	extraction	550:559	arg1	time					561:564	extraction time	550:564	extraction time (27 min)	550:573	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	5	48	theme	HPLC	919:922	arg1	analysis					924:931	the HPLC analysis	915:931	the HPLC analysis	915:931	Additionally, according to the HPLC analysis, the aAAP-1 was mainly composed of mannose, glucuronic acid, glucose, galactose, and xylose, with a molar ratio of 80.63:9.88:2.25:1:31.13.					
32041370	1	49	from	application	230:240	arg1	industry					265:272	the functional food industry	245:272	the functional food industry	245:272	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	3	50	theme	crude	754:758	arg1	cAAP					765:768	cAAP	765:768	cAAP	765:768	Furthermore, the acidic AAP (aAAP) was precipitated with cetyltrimethylammonium bromide (CTAB) from crude AAP (cAAP).					
32041370	3	50	theme	crude	754:758	arg1	AAP					760:762	crude AAP	754:762	crude AAP (cAAP)	754:769	Furthermore, the acidic AAP (aAAP) was precipitated with cetyltrimethylammonium bromide (CTAB) from crude AAP (cAAP).					
32041370	1	51	theme	AAP	348:350	arg1	extraction					334:343	the extraction	330:343	the extraction of AAP	330:350	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	0	52	from	auricula-judae	124:137	arg1	Activity					72:79	Anticoagulant Activity	58:79	Anticoagulant Activity	58:79	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	0	52	from	auricula-judae	124:137	arg1	Polysaccharides					91:105	Acidic Polysaccharides	84:105	Acidic Polysaccharides from Auricularia auricula-judae	84:137	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	0	52	from	auricula-judae	124:137	arg1	Optimization					11:22	Extraction Optimization	0:22	Extraction Optimization	0:22	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	0	52	from	auricula-judae	124:137	arg1	Characterization					36:51	Structural Characterization	25:51	Structural Characterization	25:51	Extraction Optimization, Structural Characterization, and Anticoagulant Activity of Acidic Polysaccharides from Auricularia auricula-judae.					
32041370	1	53	theme	Auricularia	151:161	arg1	anticoagulants					211:224	natural anticoagulants	203:224	natural anticoagulants	203:224	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	1	53	theme	Auricularia	151:161	arg1	AAP					195:197	AAP	195:197	AAP	195:197	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	1	53	theme	Auricularia	151:161	arg1	polysaccharides					178:192	Auricularia auricula-judae polysaccharides	151:192	Auricularia auricula-judae polysaccharides (AAP)	151:198	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	1	54	theme	food	260:263	arg1	industry					265:272	the functional food industry	245:272	the functional food industry	245:272	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	6	55	theme	endogenous	1290:1299	arg1	pathway					1315:1321	the endogenous and exogenous pathway	1286:1321	the endogenous and exogenous pathway	1286:1321	Moreover, the results of the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) indicated aAAP-1 had anticoagulant activity, which was a synergic anticoagulant activity by the endogenous and exogenous pathway.					
32041370	4	56	theme	Fast	853:856	arg1	column					863:868	a DEAE Purose 6 Fast Flow column	837:868	a DEAE Purose 6 Fast Flow column	837:868	aAAP was further purified using ion exchange chromatography with a DEAE Purose 6 Fast Flow column to obtain aAAP-1.					
32041370	2	57	theme	extraction	411:420	arg1	yield					422:426	The maximum extraction yield	399:426	The maximum extraction yield of crude AAP (14.74 mg/g)	399:452	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	2	58	theme	extraction	484:493	arg1	parameters					495:504	the optimized extraction parameters	470:504	the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W)	470:651	The maximum extraction yield of crude AAP (14.74 mg/g) was obtained at the optimized extraction parameters as follows: Extraction temperature (74 °C), extraction time (27 min), the ratio of liquid to raw material (103 mL/g), and ultrasound power (198 W).					
32041370	5	59	theme	glucuronic	977:986	arg1	acid					988:991	glucuronic acid	977:991	glucuronic acid	977:991	Additionally, according to the HPLC analysis, the aAAP-1 was mainly composed of mannose, glucuronic acid, glucose, galactose, and xylose, with a molar ratio of 80.63:9.88:2.25:1:31.13.					
32041370	1	60	theme	ultrasound	275:284	arg1	extraction					295:304	ultrasound assisted extraction	275:304	ultrasound assisted extraction (UAE)	275:310	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
32041370	1	60	theme	ultrasound	275:284	arg1	UAE					307:309	UAE	307:309	UAE	307:309	To explore Auricularia auricula-judae polysaccharides (AAP) as natural anticoagulants for application in the functional food industry, ultrasound assisted extraction (UAE) was optimized for the extraction of AAP by using a response surface methodology (RSM).					
34710756	13	0	theme	rats	2816:2819	arg1	treatment					2799:2807	the treatment	2795:2807	the treatment of SDS rats	2795:2819	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	9	1	theme	lymphocyte	1929:1938	arg1	cells					1940:1944	T lymphocyte cells	1927:1944	T lymphocyte cells	1927:1944	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	6	2	theme	SCFAs	1166:1170	arg1	levels					1156:1161	The levels	1152:1161	The levels of SCFAs in feces and serum	1152:1189	The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing.					
34710756	12	3	theme	Collinsella	2624:2634	arg1	abundance					2593:2601	the abundance	2589:2601	the abundance of Butyricimonas and Collinsella, which were different from altered genera in the SJZD group	2589:2694	S-3 significantly increased the abundance of Butyricimonas and Collinsella, which were different from altered genera in the SJZD group.					
34710756	5	4	theme	intestinal	1082:1091	arg1	lymphocyte					1095:1104	the intestinal T lymphocyte	1078:1104	the intestinal T lymphocyte expression	1078:1115	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	3	5	from	components	571:580	arg1	SJZD					585:588	SJZD	585:588	SJZD	585:588	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	3	6	theme	SDS	657:659	arg1	rats					661:664	SDS rats	657:664	SDS rats	657:664	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	1	7	theme	Song	233:236	arg1	dynasty					238:244	the Song dynasty	229:244	the Song dynasty	229:244	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	7	8	theme	pseudo-germ-free	1340:1355	arg1	rats					1404:1407	pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats	1340:1407	pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats	1340:1407	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	4	9	theme	reserpine	766:774	arg1	injection					776:784	reserpine injection	766:784	reserpine injection	766:784	STUDY DESIGN AND METHODS First, SDS model rats were established by reserpine injection and then treated with SJZD, NPS and S-3.					
34710756	3	10	from	SJZD	585:588	arg1	effects					550:556	the effects	546:556	the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms	546:696	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	6	11	theme	RNA	1311:1313	arg1	sequencing					1315:1324	RNA sequencing	1311:1324	RNA sequencing	1311:1324	The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing.					
34710756	8	12	theme	stronger	1538:1545	arg1	effect					1559:1564	a stronger therapeutic effect	1536:1564	a stronger therapeutic effect on intestinal immune and GI hormone secretion	1536:1610	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	5	13	theme	western	1060:1066	arg1	blot					1068:1071	western blot	1060:1071	western blot	1060:1071	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	9	14	theme	hormones	1727:1734	arg1	secretion					1711:1719	the secretion	1707:1719	the secretion of GI hormones in SDS rats	1707:1746	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	3	15	theme	underlying	676:685	arg1	mechanisms					687:696	their underlying mechanisms	670:696	their underlying mechanisms	670:696	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	12	16	from	genera	2671:2676	arg1	group					2690:2694	the SJZD group	2681:2694	the SJZD group	2681:2694	S-3 significantly increased the abundance of Butyricimonas and Collinsella, which were different from altered genera in the SJZD group.					
34710756	12	16	from	genera	2671:2676	arg1	different					2648:2656	different	2648:2656	different	2648:2656	S-3 significantly increased the abundance of Butyricimonas and Collinsella, which were different from altered genera in the SJZD group.					
34710756	5	17	from	levels	921:926	arg1	expressions					968:978	their related receptor expressions	945:978	their related receptor expressions in rat intestine	945:995	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	5	17	from	levels	921:926	arg1	serum					935:939	rat serum	931:939	rat serum	931:939	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	7	18	theme	SDS	1491:1493	arg1	treatment					1478:1486	the treatment	1474:1486	the treatment of SDS with SJZD, NPS and S-3	1474:1516	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	9	19	theme	SDS	1739:1741	arg1	rats					1743:1746	SDS rats	1739:1746	SDS rats	1739:1746	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	10	20	theme	hormones	2272:2279	arg1	regulation					2255:2264	the regulation	2251:2264	the regulation of GI hormones by SJZD and NPS	2251:2295	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	20	theme	hormones	2272:2279	arg1	dependent					2315:2323	dependent	2315:2323	dependent	2315:2323	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	6	21	theme	gas	1208:1210	arg1	GC-MS					1246:1250	GC-MS	1246:1250	GC-MS	1246:1250	The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing.					
34710756	6	21	theme	gas	1208:1210	arg1	spectrometry					1232:1243	gas chromatography-mass spectrometry	1208:1243	gas chromatography-mass spectrometry (GC-MS)	1208:1251	The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing.					
34710756	5	22	theme	related	951:957	arg1	expressions					968:978	their related receptor expressions	945:978	their related receptor expressions in rat intestine	945:995	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	8	23	theme	intestinal	1569:1578	arg1	secretion					1602:1610	intestinal immune and GI hormone secretion	1569:1610	intestinal immune and GI hormone secretion	1569:1610	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	10	24	theme	NPS	2118:2120	arg1	regulation					2151:2160	the regulation	2147:2160	the regulation of GI hormones of S-3	2147:2182	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	24	theme	NPS	2118:2120	arg1	effects					2101:2107	the immune-enhancing effects	2080:2107	the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats	2080:2141	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	24	theme	NPS	2118:2120	arg1	related					2188:2194	related	2188:2194	related	2188:2194	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	1	25	theme	Chinese	342:348	arg1	TCM					360:362	TCM	360:362	TCM	360:362	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	1	25	theme	Chinese	342:348	arg1	medicine					350:357	traditional Chinese medicine	330:357	traditional Chinese medicine (TCM)	330:363	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	13	26	theme	effective	2762:2770	arg1	substances					2772:2781	inseparable effective substances	2750:2781	inseparable effective substances	2750:2781	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	13	26	theme	effective	2762:2770	arg1	S-3					2742:2744	S-3	2742:2744	S-3	2742:2744	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	13	26	theme	effective	2762:2770	arg1	NPS					2734:2736	NPS	2734:2736	NPS	2734:2736	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	10	27	from	regulation	2151:2160	arg1	rats					2138:2141	SDS rats	2134:2141	SDS rats	2134:2141	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	14	28	from	effect	3014:3019	arg1	hormones					3048:3055	gastrointestinal hormones	3031:3055	gastrointestinal hormones	3031:3055	The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.					
34710756	9	29	theme	cells	1940:1944	arg1	expression					1913:1922	the expression	1909:1922	the expression of T lymphocyte cells	1909:1944	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	5	30	theme	rat	931:933	arg1	serum					935:939	rat serum	931:939	rat serum	931:939	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	8	31	theme	hormone	1594:1600	arg1	secretion					1602:1610	intestinal immune and GI hormone secretion	1569:1610	intestinal immune and GI hormone secretion	1569:1610	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	7	32	theme	gut	1456:1458	arg1	microbiota					1460:1469	the gut microbiota	1452:1469	the gut microbiota	1452:1469	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	13	33	theme	intestinal	2907:2916	arg1	immunity					2918:2925	intestinal immunity	2907:2925	intestinal immunity	2907:2925	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	1	34	theme	Sijunzi	155:161	arg1	prescription					260:271	the basic prescription	250:271	the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM)	250:363	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	1	34	theme	Sijunzi	155:161	arg1	SJZD					174:177	SJZD	174:177	SJZD	174:177	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	1	34	theme	Sijunzi	155:161	arg1	decoction					163:171	BACKGROUND Sijunzi decoction	144:171	BACKGROUND Sijunzi decoction (SJZD)	144:178	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	9	35	from	secretion	1711:1719	arg1	rats					1743:1746	SDS rats	1739:1746	SDS rats	1739:1746	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	0	36	theme	Sijunzi	74:80	arg1	decoction					82:90	Sijunzi decoction	74:90	Sijunzi decoction	74:90	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.					
34710756	14	37	theme	microbiota	2960:2969	arg1	effect					2942:2947	The mediation effect	2928:2947	The mediation effect of the gut microbiota	2928:2969	The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.					
34710756	14	37	theme	microbiota	2960:2969	arg1	important					2984:2992	important	2984:2992	important	2984:2992	The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.					
34710756	7	38	theme	key	1440:1442	arg1	role					1444:1447	the key role	1436:1447	the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3	1436:1516	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	14	39	theme	gastrointestinal	3031:3046	arg1	hormones					3048:3055	gastrointestinal hormones	3031:3055	gastrointestinal hormones	3031:3055	The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.					
34710756	5	40	from	serum	935:939	arg1	intestine					987:995	rat intestine	983:995	rat intestine	983:995	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	1	41	theme	spleen	294:299	arg1	SDS					322:324	SDS	322:324	SDS	322:324	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	1	41	theme	spleen	294:299	arg1	syndrome					312:319	spleen deficiency syndrome	294:319	spleen deficiency syndrome (SDS)	294:325	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	3	42	dep	PURPOSE	509:515	arg1	sought					528:533	sought	528:533	sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms	528:696	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	10	43	theme	S-3	2180:2182	arg1	hormones					2168:2175	GI hormones	2165:2175	GI hormones of S-3	2165:2182	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	9	44	theme	T	1822:1822	arg1	cells					1835:1839	T lymphocyte cells	1822:1839	T lymphocyte cells	1822:1839	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	4	45	theme	STUDY	699:703	arg1	DESIGN					705:710	STUDY DESIGN	699:710	STUDY DESIGN	699:710	STUDY DESIGN AND METHODS First, SDS model rats were established by reserpine injection and then treated with SJZD, NPS and S-3.					
34710756	0	46	theme	deficiency	102:111	arg1	syndrome					113:120	spleen deficiency syndrome	95:120	spleen deficiency syndrome	95:120	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.					
34710756	5	47	theme	rat	983:985	arg1	intestine					987:995	rat intestine	983:995	rat intestine	983:995	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	5	48	theme	immune	870:875	arg1	function					877:884	immune function	870:884	immune function	870:884	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	10	49	theme	hormones	2168:2175	arg1	regulation					2151:2160	the regulation	2147:2160	the regulation of GI hormones of S-3	2147:2182	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	49	theme	hormones	2168:2175	arg1	effects					2101:2107	the immune-enhancing effects	2080:2107	the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats	2080:2141	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	49	theme	hormones	2168:2175	arg1	related					2188:2194	related	2188:2194	related	2188:2194	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	9	50	theme	cells	1835:1839	arg1	expression					1808:1817	the expression	1804:1817	the expression of T lymphocyte cells	1804:1839	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	5	51	theme	lymphocyte	1095:1104	arg1	expression					1106:1115	the intestinal T lymphocyte expression	1078:1115	the intestinal T lymphocyte expression	1078:1115	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	8	52	theme	NPS	1647:1649	arg1	efficacy					1635:1642	the efficacy	1631:1642	the efficacy of NPS and S-3	1631:1657	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	14	53	theme	regulating	3003:3012	arg1	effect					3014:3019	the regulating effect	2999:3019	the regulating effect of NPS on gastrointestinal hormones	2999:3055	The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.					
34710756	10	54	theme	GMD	2054:2056	arg1	rats					2058:2061	GMD rats	2054:2061	GMD rats	2054:2061	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	0	55	theme	Differential	0:11	arg1	effect					13:18	Differential effect	0:18	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.	0:142	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.					
34710756	1	56	theme	dynasty	238:244	arg1	"					224:224	"Tai Ping Hui Min He Ji Ju Fang"	193:224	"Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty	193:244	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	8	57	theme	S-3	1655:1657	arg1	efficacy					1635:1642	the efficacy	1631:1642	the efficacy of NPS and S-3	1631:1657	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	10	58	theme	SJZD	2112:2115	arg1	regulation					2151:2160	the regulation	2147:2160	the regulation of GI hormones of S-3	2147:2182	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	58	theme	SJZD	2112:2115	arg1	effects					2101:2107	the immune-enhancing effects	2080:2107	the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats	2080:2141	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	58	theme	SJZD	2112:2115	arg1	related					2188:2194	related	2188:2194	related	2188:2194	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	8	59	dep	SJZD	1527:1530	arg1	has					1532:1534	has	1532:1534	has	1532:1534	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	7	60	theme	model	1398:1402	arg1	rats					1404:1407	pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats	1340:1407	pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats	1340:1407	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	13	61	theme	intestinal	2851:2860	arg1	motility					2862:2869	intestinal motility dysfunction and S-3	2851:2889	motility	2862:2869	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	10	62	theme	microbiota	2221:2230	arg1	composition					2232:2242	the gut microbiota composition	2213:2242	the gut microbiota composition	2213:2242	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	9	63	theme	intestinal	1868:1877	arg1	function					1886:1893	intestinal immune function	1868:1893	intestinal immune function	1868:1893	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	1	64	theme	basic	254:258	arg1	prescription					260:271	the basic prescription	250:271	the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM)	250:363	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	1	64	theme	basic	254:258	arg1	decoction					163:171	BACKGROUND Sijunzi decoction	144:171	BACKGROUND Sijunzi decoction (SJZD)	144:178	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	5	65	theme	hormone	913:919	arg1	levels					921:926	the gastrointestinal (GI) hormone levels	887:926	the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine	887:995	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	3	66	theme	components	571:580	arg1	effects					550:556	the effects	546:556	the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms	546:696	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	0	67	theme	components	60:69	arg1	effect					13:18	Differential effect	0:18	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.	0:142	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.					
34710756	7	68	theme	dysbiosis	1382:1390	arg1	rats					1404:1407	pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats	1340:1407	pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats	1340:1407	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	9	69	theme	immune	1879:1884	arg1	function					1886:1893	intestinal immune function	1868:1893	intestinal immune function	1868:1893	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	7	70	theme	gut	1367:1369	arg1	dysbiosis					1382:1390	gut microbiota dysbiosis	1367:1390	gut microbiota dysbiosis (GMD)	1367:1396	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	7	70	theme	gut	1367:1369	arg1	GMD					1393:1395	GMD	1393:1395	GMD	1393:1395	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	5	71	theme	gastrointestinal	891:906	arg1	levels					921:926	the gastrointestinal (GI) hormone levels	887:926	the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine	887:995	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	4	72	theme	SDS	731:733	arg1	rats					741:744	SDS model rats	731:744	SDS model rats	731:744	STUDY DESIGN AND METHODS First, SDS model rats were established by reserpine injection and then treated with SJZD, NPS and S-3.					
34710756	13	73	theme	SDS	2812:2814	arg1	rats					2816:2819	SDS rats	2812:2819	SDS rats	2812:2819	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	9	74	theme	T	1927:1927	arg1	cells					1940:1944	T lymphocyte cells	1927:1944	T lymphocyte cells	1927:1944	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	8	75	theme	slight	1666:1671	arg1	differences					1673:1683	slight differences	1666:1683	slight differences	1666:1683	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	13	76	from	SJZD	2787:2790	arg1	treatment					2799:2807	the treatment	2795:2807	the treatment of SDS rats	2795:2819	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	3	77	theme	active	627:632	arg1	S-3					649:651	active polysaccharide S-3	627:651	active polysaccharide S-3	627:651	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	14	78	theme	gut	2956:2958	arg1	microbiota					2960:2969	the gut microbiota	2952:2969	the gut microbiota	2952:2969	The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.					
34710756	5	79	theme	T	1093:1093	arg1	lymphocyte					1095:1104	the intestinal T lymphocyte	1078:1104	the intestinal T lymphocyte expression	1078:1115	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	6	80	theme	microbiota	1266:1275	arg1	composition					1277:1287	the gut microbiota composition	1258:1287	the gut microbiota composition	1258:1287	The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing.					
34710756	9	81	theme	GI	1724:1725	arg1	hormones					1727:1734	GI hormones	1724:1734	GI hormones	1724:1734	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	9	82	theme	intestinal	1964:1973	arg1	barrier					1975:1981	the intestinal barrier	1960:1981	the intestinal barrier in both direct and indirect ways	1960:2014	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	9	83	from	barrier	1975:1981	arg1	ways					2011:2014	both direct and indirect ways	1986:2014	both direct and indirect ways	1986:2014	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	5	84	theme	immunosorbent	1028:1040	arg1	ELISA					1049:1053	ELISA	1049:1053	ELISA	1049:1053	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	5	84	theme	immunosorbent	1028:1040	arg1	assay					1042:1046	enzyme-linked immunosorbent assay	1014:1046	enzyme-linked immunosorbent assay (ELISA)	1014:1054	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	10	85	theme	immune-enhancing	2084:2099	arg1	effects					2101:2107	the immune-enhancing effects	2080:2107	the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats	2080:2141	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	85	theme	immune-enhancing	2084:2099	arg1	related					2188:2194	related	2188:2194	related	2188:2194	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	86	theme	composition	2232:2242	arg1	modulation					2199:2208	modulation	2199:2208	modulation of the gut microbiota composition	2199:2242	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	6	87	theme	chromatography-mass	1212:1230	arg1	GC-MS					1246:1250	GC-MS	1246:1250	GC-MS	1246:1250	The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing.					
34710756	6	87	theme	chromatography-mass	1212:1230	arg1	spectrometry					1232:1243	gas chromatography-mass spectrometry	1208:1243	gas chromatography-mass spectrometry (GC-MS)	1208:1251	The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing.					
34710756	8	88	theme	therapeutic	1547:1557	arg1	effect					1559:1564	a stronger therapeutic effect	1536:1564	a stronger therapeutic effect on intestinal immune and GI hormone secretion	1536:1610	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	4	89	dep	established	751:761	arg1	First					724:728	First	724:728	First	724:728	STUDY DESIGN AND METHODS First, SDS model rats were established by reserpine injection and then treated with SJZD, NPS and S-3.					
34710756	10	90	theme	GI	2269:2270	arg1	hormones					2272:2279	GI hormones	2269:2279	GI hormones	2269:2279	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	9	91	theme	direct	1991:1996	arg1	ways					2011:2014	both direct and indirect ways	1986:2014	both direct and indirect ways	1986:2014	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	5	92	theme	receptor	959:966	arg1	expressions					968:978	their related receptor expressions	945:978	their related receptor expressions in rat intestine	945:995	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	7	93	theme	microbiota	1460:1469	arg1	role					1444:1447	the key role	1436:1447	the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3	1436:1516	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	14	94	theme	gut	3084:3086	arg1	microbiota					3088:3097	the gut microbiota	3080:3097	the gut microbiota	3080:3097	The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.					
34710756	5	95	dep	gastrointestinal	891:906	arg1	GI					909:910	GI	909:910	GI	909:910	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	9	96	theme	indirect	2002:2009	arg1	ways					2011:2014	both direct and indirect ways	1986:2014	both direct and indirect ways	1986:2014	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	8	97	theme	immune	1580:1585	arg1	secretion					1602:1610	intestinal immune and GI hormone secretion	1569:1610	intestinal immune and GI hormone secretion	1569:1610	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	10	98	theme	SDS	2134:2136	arg1	rats					2138:2141	SDS rats	2134:2141	SDS rats	2134:2141	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	9	99	theme	intestinal	1770:1779	arg1	immunity					1781:1788	intestinal immunity	1770:1788	intestinal immunity	1770:1788	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	13	100	dep	motility	2862:2869	arg1	dysfunction					2871:2881	dysfunction	2871:2881	dysfunction	2871:2881	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	8	101	theme	GI	1591:1592	arg1	secretion					1602:1610	intestinal immune and GI hormone secretion	1569:1610	intestinal immune and GI hormone secretion	1569:1610	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	1	102	theme	BACKGROUND	144:153	arg1	prescription					260:271	the basic prescription	250:271	the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM)	250:363	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	1	102	theme	BACKGROUND	144:153	arg1	SJZD					174:177	SJZD	174:177	SJZD	174:177	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	1	102	theme	BACKGROUND	144:153	arg1	decoction					163:171	BACKGROUND Sijunzi decoction	144:171	BACKGROUND Sijunzi decoction (SJZD)	144:178	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	10	103	theme	S-3	2127:2129	arg1	regulation					2151:2160	the regulation	2147:2160	the regulation of GI hormones of S-3	2147:2182	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	103	theme	S-3	2127:2129	arg1	effects					2101:2107	the immune-enhancing effects	2080:2107	the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats	2080:2141	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	103	theme	S-3	2127:2129	arg1	related					2188:2194	related	2188:2194	related	2188:2194	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	104	from	experiments	2031:2041	arg1	pGF					2046:2048	pGF	2046:2048	pGF	2046:2048	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	104	from	experiments	2031:2041	arg1	rats					2058:2061	GMD rats	2054:2061	GMD rats	2054:2061	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	13	105	dep	CONCLUSION	2697:2706	arg1	enhances					2898:2905	enhances	2898:2905	enhances intestinal immunity	2898:2925	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	6	106	theme	gut	1262:1264	arg1	composition					1277:1287	the gut microbiota composition	1258:1287	the gut microbiota composition	1258:1287	The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing.					
34710756	5	107	theme	flow	1136:1139	arg1	cytometry					1141:1149	flow cytometry	1136:1149	flow cytometry	1136:1149	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	0	108	theme	spleen	95:100	arg1	syndrome					113:120	spleen deficiency syndrome	95:120	spleen deficiency syndrome	95:120	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.					
34710756	5	109	theme	enzyme-linked	1014:1026	arg1	ELISA					1049:1053	ELISA	1049:1053	ELISA	1049:1053	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	5	109	theme	enzyme-linked	1014:1026	arg1	assay					1042:1046	enzyme-linked immunosorbent assay	1014:1046	enzyme-linked immunosorbent assay (ELISA)	1014:1054	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	8	110	theme	SDS	1615:1617	arg1	rats					1619:1622	SDS rats	1615:1622	SDS rats	1615:1622	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	1	111	theme	syndrome	312:319	arg1	treatment					281:289	the treatment	277:289	the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM)	277:363	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	0	112	from	effect	13:18	arg1	syndrome					113:120	spleen deficiency syndrome	95:120	spleen deficiency syndrome	95:120	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.					
34710756	0	112	from	effect	13:18	arg1	mechanisms					132:141	their mechanisms	126:141	their mechanisms	126:141	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.					
34710756	0	112	from	effect	13:18	arg1	decoction					82:90	Sijunzi decoction	74:90	Sijunzi decoction	74:90	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.					
34710756	5	113	from	expressions	968:978	arg1	intestine					987:995	rat intestine	983:995	rat intestine	983:995	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	1	114	theme	deficiency	301:310	arg1	SDS					322:324	SDS	322:324	SDS	322:324	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	1	114	theme	deficiency	301:310	arg1	syndrome					312:319	spleen deficiency syndrome	294:319	spleen deficiency syndrome (SDS)	294:325	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	14	115	theme	NPS	3024:3026	arg1	effect					3014:3019	the regulating effect	2999:3019	the regulating effect of NPS on gastrointestinal hormones	2999:3055	The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.					
34710756	9	116	theme	lymphocyte	1824:1833	arg1	cells					1835:1839	T lymphocyte cells	1822:1839	T lymphocyte cells	1822:1839	NPS mainly regulated the secretion of GI hormones in SDS rats and directly improved intestinal immunity by increasing the expression of T lymphocyte cells, while S-3 mainly enhanced intestinal immune function by increasing the expression of T lymphocyte cells and repairing the intestinal barrier in both direct and indirect ways.					
34710756	14	117	theme	mediation	2932:2940	arg1	effect					2942:2947	The mediation effect	2928:2947	The mediation effect of the gut microbiota	2928:2969	The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.					
34710756	14	117	theme	mediation	2932:2940	arg1	important					2984:2992	important	2984:2992	important	2984:2992	The mediation effect of the gut microbiota is extremely important, but the regulating effect of NPS on gastrointestinal hormones has nothing to do with the gut microbiota.					
34710756	10	118	theme	GI	2165:2166	arg1	hormones					2168:2175	GI hormones	2165:2175	GI hormones of S-3	2165:2182	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	3	119	from	effects	550:556	arg1	SJZD					585:588	SJZD	585:588	SJZD	585:588	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	3	119	from	effects	550:556	arg1	mechanisms					687:696	their underlying mechanisms	670:696	their underlying mechanisms	670:696	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	3	119	from	effects	550:556	arg1	rats					661:664	SDS rats	657:664	SDS rats	657:664	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	12	120	theme	SJZD	2685:2688	arg1	group					2690:2694	the SJZD group	2681:2694	the SJZD group	2681:2694	S-3 significantly increased the abundance of Butyricimonas and Collinsella, which were different from altered genera in the SJZD group.					
34710756	7	121	with	treatment	1478:1486	arg1	S-3					1514:1516	S-3	1514:1516	S-3	1514:1516	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	7	121	with	treatment	1478:1486	arg1	NPS					1506:1508	NPS	1506:1508	NPS	1506:1508	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	7	121	with	treatment	1478:1486	arg1	SJZD					1500:1503	SJZD	1500:1503	SJZD	1500:1503	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	1	122	theme	traditional	330:340	arg1	TCM					360:362	TCM	360:362	TCM	360:362	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	1	122	theme	traditional	330:340	arg1	medicine					350:357	traditional Chinese medicine	330:357	traditional Chinese medicine (TCM)	330:363	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	0	123	theme	polysaccharide	23:36	arg1	effect					13:18	Differential effect	0:18	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.	0:142	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.					
34710756	8	124	from	effect	1559:1564	arg1	secretion					1602:1610	intestinal immune and GI hormone secretion	1569:1610	intestinal immune and GI hormone secretion	1569:1610	RESULTS SJZD has a stronger therapeutic effect on intestinal immune and GI hormone secretion in SDS rats, while the efficacy of NPS and S-3 showed slight differences.					
34710756	2	125	dep	Poria	447:451	arg1	Melle					502:506	Melle	502:506	Melle	502:506	It is composed of Ginseng Radix et Rhizoma, Atractylodisa Macricephalae Rhizoma, Poria and Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle.					
34710756	5	126	theme	GI	854:855	arg1	motility					857:864	GI motility	854:864	GI motility	854:864	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	12	127	theme	altered	2663:2669	arg1	genera					2671:2676	altered genera	2663:2676	altered genera in the SJZD group	2663:2694	S-3 significantly increased the abundance of Butyricimonas and Collinsella, which were different from altered genera in the SJZD group.					
34710756	0	128	theme	nonpolysaccharide	42:58	arg1	components					60:69	nonpolysaccharide components	42:69	nonpolysaccharide components	42:69	Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.					
34710756	2	129	dep	Glycyrrhizae	457:468	arg1	et					476:477	Glycyrrhizae Radix et	457:477	Glycyrrhizae Radix et	457:477	It is composed of Ginseng Radix et Rhizoma, Atractylodisa Macricephalae Rhizoma, Poria and Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle.					
34710756	5	130	link	enzyme-linked	1014:1026	arg1	ELISA					1049:1053	ELISA	1049:1053	ELISA	1049:1053	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	5	130	link	enzyme-linked	1014:1026	arg1	assay					1042:1046	enzyme-linked immunosorbent assay	1014:1046	enzyme-linked immunosorbent assay (ELISA)	1014:1054	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	10	131	theme	gut	2217:2219	arg1	composition					2232:2242	the gut microbiota composition	2213:2242	the gut microbiota composition	2213:2242	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	10	132	from	effects	2101:2107	arg1	rats					2138:2141	SDS rats	2134:2141	SDS rats	2134:2141	Additionally, experiments in pGF and GMD rats have proven that the immune-enhancing effects of SJZD, NPS, and S-3 on SDS rats and the regulation of GI hormones of S-3 are related to modulation of the gut microbiota composition, while the regulation of GI hormones by SJZD and NPS is not completely dependent on this modulation.					
34710756	3	133	theme	different	561:569	arg1	S-3					649:651	active polysaccharide S-3	627:651	active polysaccharide S-3	627:651	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	3	133	theme	different	561:569	arg1	components					571:580	different components	561:580	different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3)	561:652	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	3	133	theme	different	561:569	arg1	NPS					619:621	nonpolysaccharide NPS	601:621	nonpolysaccharide NPS	601:621	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	4	134	theme	model	735:739	arg1	rats					741:744	SDS model rats	731:744	SDS model rats	731:744	STUDY DESIGN AND METHODS First, SDS model rats were established by reserpine injection and then treated with SJZD, NPS and S-3.					
34710756	1	135	from	treatment	281:289	arg1	TCM					360:362	TCM	360:362	TCM	360:362	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	1	135	from	treatment	281:289	arg1	medicine					350:357	traditional Chinese medicine	330:357	traditional Chinese medicine (TCM)	330:363	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34710756	13	136	theme	inseparable	2750:2760	arg1	substances					2772:2781	inseparable effective substances	2750:2781	inseparable effective substances	2750:2781	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	13	136	theme	inseparable	2750:2760	arg1	S-3					2742:2744	S-3	2742:2744	S-3	2742:2744	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	13	136	theme	inseparable	2750:2760	arg1	NPS					2734:2736	NPS	2734:2736	NPS	2734:2736	CONCLUSION This study uncovered that NPS and S-3 are inseparable effective substances for SJZD in the treatment of SDS rats, in which NPS mainly improves intestinal motility dysfunction and S-3 mainly enhances intestinal immunity.					
34710756	3	137	theme	nonpolysaccharide	601:617	arg1	NPS					619:621	nonpolysaccharide NPS	601:621	nonpolysaccharide NPS	601:621	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	7	138	from	role	1444:1447	arg1	treatment					1478:1486	the treatment	1474:1486	the treatment of SDS with SJZD, NPS and S-3	1474:1516	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	5	139	from	effect	844:849	arg1	motility					857:864	GI motility	854:864	GI motility	854:864	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	5	139	from	effect	844:849	arg1	function					877:884	immune function	870:884	immune function	870:884	To clarify their effect on GI motility and immune function, the gastrointestinal (GI) hormone levels in rat serum and their related receptor expressions in rat intestine were detected by enzyme-linked immunosorbent assay (ELISA) and western blot, and the intestinal T lymphocyte expression were quantified by flow cytometry.					
34710756	6	140	from	levels	1156:1161	arg1	serum					1185:1189	serum	1185:1189	serum	1185:1189	The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing.					
34710756	6	140	from	levels	1156:1161	arg1	feces					1175:1179	feces	1175:1179	feces	1175:1179	The levels of SCFAs in feces and serum were measured by gas chromatography-mass spectrometry (GC-MS), and the gut microbiota composition was determined by 16S RNA sequencing.					
34710756	7	141	theme	microbiota	1371:1380	arg1	dysbiosis					1382:1390	gut microbiota dysbiosis	1367:1390	gut microbiota dysbiosis (GMD)	1367:1396	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	7	141	theme	microbiota	1371:1380	arg1	GMD					1393:1395	GMD	1393:1395	GMD	1393:1395	Furthermore, pseudo-germ-free (pGF) and gut microbiota dysbiosis (GMD) model rats were established to verify the key role of the gut microbiota in the treatment of SDS with SJZD, NPS and S-3.					
34710756	3	142	theme	polysaccharide	634:647	arg1	S-3					649:651	active polysaccharide S-3	627:651	active polysaccharide S-3	627:651	PURPOSE This study sought to explore the effects of different components in SJZD (including nonpolysaccharide NPS and active polysaccharide S-3) on SDS rats and their underlying mechanisms.					
34710756	12	143	theme	Butyricimonas	2606:2618	arg1	abundance					2593:2601	the abundance	2589:2601	the abundance of Butyricimonas and Collinsella, which were different from altered genera in the SJZD group	2589:2694	S-3 significantly increased the abundance of Butyricimonas and Collinsella, which were different from altered genera in the SJZD group.					
34710756	1	144	theme	Fang	220:223	arg1	"					224:224	"Tai Ping Hui Min He Ji Ju Fang"	193:224	"Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty	193:244	BACKGROUND Sijunzi decoction (SJZD), reported in "Tai Ping Hui Min He Ji Ju Fang" of the Song dynasty, is the basic prescription for the treatment of spleen deficiency syndrome (SDS) in traditional Chinese medicine (TCM).					
34873736	2	0	theme	Mw	571:572	arg1	Da					585:586	Mw 1.93 × 106 Da	571:586	Mw 1.93 × 106 Da	571:586	They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP-1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP-2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%).					
34873736	2	0	theme	Mw	571:572	arg1	DLP-2					564:568	DLP-2	564:568	DLP-2 (Mw 1.93 × 106 Da)	564:587	They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP-1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP-2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%).					
34873736	9	1	theme	huge	1681:1684	arg1	biomass					1686:1692	huge biomass	1681:1692	huge biomass	1681:1692	PRACTICAL APPLICATIONS: The leaf of Dendrobium officinale is a by-product with huge biomass.					
34873736	4	2	theme	DLP-1	774:778	arg1	image					765:769	The scanning electron microscope image	732:769	The scanning electron microscope image of DLP-1	732:778	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	4	3	contain	had	895:897	arg1	they					890:893	they	890:893	they	890:893	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	4	3	contain	had	895:897	arg2	structure					912:920	an amorphous structure	899:920	an amorphous structure	899:920	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	11	4	theme	fractions	1978:1986	arg1	characteristics					1916:1930	structural characteristics	1905:1930	structural characteristics	1905:1930	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	11	4	theme	fractions	1978:1986	arg1	effects					1948:1954	immunologic effects	1936:1954	immunologic effects	1936:1954	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	10	5	theme	chemical	1734:1741	arg1	composition					1743:1753	its chemical composition	1730:1753	its chemical composition	1730:1753	The lack of systematic research on its chemical composition and pharmacologic effect, leading to a great waste of resources.					
34873736	4	6	theme	electron	745:752	arg1	microscope					754:763	The scanning electron microscope	732:763	The scanning electron microscope image of DLP-1	732:778	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	6	7	theme	resonance	1046:1054	arg1	analysis					1056:1063	Nuclear magnetic resonance analysis	1029:1063	Nuclear magnetic resonance analysis	1029:1063	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	10	8	from	lack	1699:1702	arg1	composition					1743:1753	its chemical composition	1730:1753	its chemical composition	1730:1753	The lack of systematic research on its chemical composition and pharmacologic effect, leading to a great waste of resources.					
34873736	6	9	theme	Nuclear	1029:1035	arg1	analysis					1056:1063	Nuclear magnetic resonance analysis	1029:1063	Nuclear magnetic resonance analysis	1029:1063	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	8	10	theme	excellent	1514:1522	arg1	bioactivities					1541:1553	excellent immunoregulatory bioactivities	1514:1553	excellent immunoregulatory bioactivities	1514:1553	These results proved that both DLP-1 and DLP-2 possessed excellent immunoregulatory bioactivities and could be functional food or adjuvant drug.					
34873736	7	11	theme	TNF-α	1439:1443	arg1	production					1396:1405	the production	1392:1405	the production of nitric oxide, interleukin-6, TNF-α, and IL-1β	1392:1454	Besides, DLP-1 and DLP-2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin-6, TNF-α, and IL-1β.					
34873736	11	12	from	characteristics	1916:1930	arg1	officinale					2014:2023	D. officinale	2011:2023	D. officinale leaves	2011:2030	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	6	13	theme	DLP-1	1170:1174	arg1	-β-D-Glcp					1098:1106	(1→4)-β-D-Glcp	1093:1106	(1→4)-β-D-Glcp	1093:1106	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	13	theme	DLP-1	1170:1174	arg1	-β-D-Manp					1082:1090	(1→4)-β-D-Manp	1077:1090	(1→4)-β-D-Manp	1077:1090	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	13	theme	DLP-1	1170:1174	arg1	types					1161:1165	the main linkage types	1144:1165	the main linkage types of DLP-1	1144:1174	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	13	theme	DLP-1	1170:1174	arg1	-2-O-acetyl-β-D-Manp					1118:1137	(1→4)-2-O-acetyl-β-D-Manp	1113:1137	(1→4)-2-O-acetyl-β-D-Manp	1113:1137	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	7	14	theme	interleukin-6	1424:1436	arg1	production					1396:1405	the production	1392:1405	the production of nitric oxide, interleukin-6, TNF-α, and IL-1β	1392:1454	Besides, DLP-1 and DLP-2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin-6, TNF-α, and IL-1β.					
34873736	3	15	theme	triple-helical	673:686	arg1	conformation					688:699	A triple-helical conformation	671:699	A triple-helical conformation	671:699	A triple-helical conformation was exhibited by both of them.					
34873736	5	16	theme	rough	953:957	arg1	surface					978:984	a rough, loose, and uneven surface	951:984	a rough, loose, and uneven surface consisting of large sponge-like particles	951:1026	In contrast, DLP-2 revealed a rough, loose, and uneven surface consisting of large sponge-like particles.					
34873736	7	17	theme	oxide	1417:1421	arg1	production					1396:1405	the production	1392:1405	the production of nitric oxide, interleukin-6, TNF-α, and IL-1β	1392:1454	Besides, DLP-1 and DLP-2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin-6, TNF-α, and IL-1β.					
34873736	11	18	theme	D.	1854:1855	arg1	officinale					1857:1866	D. officinale	1854:1866	D. officinale	1854:1866	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	6	19	theme	main	1148:1151	arg1	-β-D-Glcp					1098:1106	(1→4)-β-D-Glcp	1093:1106	(1→4)-β-D-Glcp	1093:1106	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	19	theme	main	1148:1151	arg1	-β-D-Manp					1082:1090	(1→4)-β-D-Manp	1077:1090	(1→4)-β-D-Manp	1077:1090	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	19	theme	main	1148:1151	arg1	types					1161:1165	the main linkage types	1144:1165	the main linkage types of DLP-1	1144:1174	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	19	theme	main	1148:1151	arg1	-2-O-acetyl-β-D-Manp					1118:1137	(1→4)-2-O-acetyl-β-D-Manp	1113:1137	(1→4)-2-O-acetyl-β-D-Manp	1113:1137	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	8	20	theme	functional	1568:1577	arg1	food					1579:1582	functional food	1568:1582	functional food	1568:1582	These results proved that both DLP-1 and DLP-2 possessed excellent immunoregulatory bioactivities and could be functional food or adjuvant drug.					
34873736	4	21	theme	lamellar	810:817	arg1	shape					819:823	an irregular and large lamellar shape	787:823	an irregular and large lamellar shape	787:823	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	10	22	theme	resources	1809:1817	arg1	waste					1800:1804	a great waste	1792:1804	a great waste of resources	1792:1817	The lack of systematic research on its chemical composition and pharmacologic effect, leading to a great waste of resources.					
34873736	1	23	theme	polysaccharides	140:154	arg1	DLP-2					211:215	DLP-2	211:215	DLP-2	211:215	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	1	23	theme	polysaccharides	140:154	arg1	DLP-1					201:205	DLP-1	201:205	DLP-1	201:205	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	1	23	theme	polysaccharides	140:154	arg1	kinds					131:135	two kinds	127:135	two kinds of polysaccharides from leaves of Dendrobium officinale	127:191	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	11	24	theme	anti-inflammatory	2103:2119	arg1	agents					2121:2126	anti-inflammatory agents	2103:2126	anti-inflammatory agents	2103:2126	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	0	25	theme	Dendrobium	82:91	arg1	officinale					93:102	Dendrobium officinale	82:102	Dendrobium officinale	82:102	Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves.					
34873736	1	26	theme	Sephadex	339:346	arg1	columns					354:360	DEAE-52 cellulose and Sephadex G-100 columns	317:360	columns	354:360	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	11	27	theme	officinale	1857:1866	arg1	value					1845:1849	the value	1841:1849	the value of D. officinale	1841:1866	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	8	28	contain	possessed	1504:1512	arg2	bioactivities					1541:1553	excellent immunoregulatory bioactivities	1514:1553	excellent immunoregulatory bioactivities	1514:1553	These results proved that both DLP-1 and DLP-2 possessed excellent immunoregulatory bioactivities and could be functional food or adjuvant drug.					
34873736	8	28	contain	possessed	1504:1512	arg1	DLP-2					1498:1502	DLP-2	1498:1502	DLP-2	1498:1502	These results proved that both DLP-1 and DLP-2 possessed excellent immunoregulatory bioactivities and could be functional food or adjuvant drug.					
34873736	8	28	contain	possessed	1504:1512	arg1	DLP-1					1488:1492	DLP-1	1488:1492	DLP-1	1488:1492	These results proved that both DLP-1 and DLP-2 possessed excellent immunoregulatory bioactivities and could be functional food or adjuvant drug.					
34873736	1	29	theme	Dendrobium	171:180	arg1	officinale					182:191	Dendrobium officinale	171:191	Dendrobium officinale	171:191	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	5	30	theme	loose	960:964	arg1	surface					978:984	a rough, loose, and uneven surface	951:984	a rough, loose, and uneven surface consisting of large sponge-like particles	951:1026	In contrast, DLP-2 revealed a rough, loose, and uneven surface consisting of large sponge-like particles.					
34873736	11	31	theme	D.	2011:2012	arg1	officinale					2014:2023	D. officinale	2011:2023	D. officinale leaves	2011:2030	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	4	32	theme	amorphous	902:910	arg1	structure					912:920	an amorphous structure	899:920	an amorphous structure	899:920	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	0	33	from	activity	49:56	arg1	officinale					93:102	Dendrobium officinale	82:102	Dendrobium officinale	82:102	Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves.					
34873736	11	34	from	effects	1948:1954	arg1	officinale					2014:2023	D. officinale	2011:2023	D. officinale leaves	2011:2030	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	0	35	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves.					
34873736	7	36	theme	IL-1β	1450:1454	arg1	production					1396:1405	the production	1392:1405	the production of nitric oxide, interleukin-6, TNF-α, and IL-1β	1392:1454	Besides, DLP-1 and DLP-2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin-6, TNF-α, and IL-1β.					
34873736	11	37	theme	immunologic	1936:1946	arg1	effects					1948:1954	immunologic effects	1936:1954	immunologic effects	1936:1954	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	1	38	theme	chromatographic	284:298	arg1	separation					300:309	chromatographic separation	284:309	chromatographic separation	284:309	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	11	39	from	officinale	2014:2023	arg1	fractions					1978:1986	two polysaccharide fractions	1959:1986	two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves	1959:2030	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	11	39	from	officinale	2014:2023	arg1	characteristics					1916:1930	structural characteristics	1905:1930	structural characteristics	1905:1930	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	11	39	from	officinale	2014:2023	arg1	effects					1948:1954	immunologic effects	1936:1954	immunologic effects	1936:1954	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	11	39	from	officinale	2014:2023	arg1	DLP-2					1999:2003	DLP-2	1999:2003	DLP-2	1999:2003	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	11	39	from	officinale	2014:2023	arg1	DLP-1					1989:1993	DLP-1	1989:1993	DLP-1	1989:1993	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	5	40	theme	sponge-like	1006:1016	arg1	particles					1018:1026	large sponge-like particles	1000:1026	large sponge-like particles	1000:1026	In contrast, DLP-2 revealed a rough, loose, and uneven surface consisting of large sponge-like particles.					
34873736	2	41	theme	1.38 × 106	483:492	arg1	DLP-1					473:477	DLP-1	473:477	DLP-1 (Mw 1.38 × 106 Da)	473:496	They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP-1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP-2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%).					
34873736	2	41	theme	1.38 × 106	483:492	arg1	Da					494:495	Mw 1.38 × 106 Da	480:495	Mw 1.38 × 106 Da	480:495	They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP-1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP-2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%).					
34873736	11	42	theme	structural	1905:1914	arg1	characteristics					1916:1930	structural characteristics	1905:1930	structural characteristics	1905:1930	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	0	43	theme	polysaccharides	61:75	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves.					
34873736	0	43	theme	polysaccharides	61:75	arg1	activity					49:56	immunoregulatory activity	32:56	immunoregulatory activity	32:56	Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves.					
34873736	9	44	theme	officinale	1649:1658	arg1	leaf					1630:1633	The leaf	1626:1633	The leaf of Dendrobium officinale	1626:1658	PRACTICAL APPLICATIONS: The leaf of Dendrobium officinale is a by-product with huge biomass.					
34873736	9	44	theme	officinale	1649:1658	arg1	by-product					1665:1674	a by-product	1663:1674	a by-product with huge biomass	1663:1692	PRACTICAL APPLICATIONS: The leaf of Dendrobium officinale is a by-product with huge biomass.					
34873736	6	45	theme	residues	1270:1277	arg1	-β-D-Glcp					1249:1257	(1→4)-β-D-Glcp	1244:1257	(1→4)-β-D-Glcp	1244:1257	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	45	theme	residues	1270:1277	arg1	-β-D-Manp					1233:1241	(1→4)-β-D-Manp	1228:1241	(1→4)-β-D-Manp	1228:1241	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	45	theme	residues	1270:1277	arg1	amount					1218:1223	a large amount	1210:1223	a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues	1210:1277	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	45	theme	residues	1270:1277	arg1	residues					1270:1277	other residues	1264:1277	other residues	1264:1277	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	1	46	theme	hot	235:237	arg1	extraction					245:254	hot water extraction	235:254	hot water extraction	235:254	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	11	47	theme	polysaccharide	1963:1976	arg1	fractions					1978:1986	two polysaccharide fractions	1959:1986	two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves	1959:2030	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	11	47	theme	polysaccharide	1963:1976	arg1	DLP-2					1999:2003	DLP-2	1999:2003	DLP-2	1999:2003	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	11	47	theme	polysaccharide	1963:1976	arg1	DLP-1					1989:1993	DLP-1	1989:1993	DLP-1	1989:1993	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	4	48	theme	microscope	754:763	arg1	image					765:769	The scanning electron microscope image	732:769	The scanning electron microscope image of DLP-1	732:778	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	9	49	dep	APPLICATIONS	1612:1623	arg1	leaf					1630:1633	The leaf	1626:1633	The leaf of Dendrobium officinale	1626:1658	PRACTICAL APPLICATIONS: The leaf of Dendrobium officinale is a by-product with huge biomass.					
34873736	9	49	dep	APPLICATIONS	1612:1623	arg1	by-product					1665:1674	a by-product	1663:1674	a by-product with huge biomass	1663:1692	PRACTICAL APPLICATIONS: The leaf of Dendrobium officinale is a by-product with huge biomass.					
34873736	6	50	theme	1→4	1114:1116	arg1	-β-D-Glcp					1098:1106	(1→4)-β-D-Glcp	1093:1106	(1→4)-β-D-Glcp	1093:1106	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	50	theme	1→4	1114:1116	arg1	-β-D-Manp					1082:1090	(1→4)-β-D-Manp	1077:1090	(1→4)-β-D-Manp	1077:1090	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	50	theme	1→4	1114:1116	arg1	types					1161:1165	the main linkage types	1144:1165	the main linkage types of DLP-1	1144:1174	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	50	theme	1→4	1114:1116	arg1	-2-O-acetyl-β-D-Manp					1118:1137	(1→4)-2-O-acetyl-β-D-Manp	1113:1137	(1→4)-2-O-acetyl-β-D-Manp	1113:1137	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	51	theme	-β-D-Glcp	1249:1257	arg1	-β-D-Glcp					1249:1257	(1→4)-β-D-Glcp	1244:1257	(1→4)-β-D-Glcp	1244:1257	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	51	theme	-β-D-Glcp	1249:1257	arg1	-β-D-Manp					1233:1241	(1→4)-β-D-Manp	1228:1241	(1→4)-β-D-Manp	1228:1241	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	51	theme	-β-D-Glcp	1249:1257	arg1	amount					1218:1223	a large amount	1210:1223	a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues	1210:1277	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	51	theme	-β-D-Glcp	1249:1257	arg1	residues					1270:1277	other residues	1264:1277	other residues	1264:1277	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	1	52	theme	ethanol	257:263	arg1	sedimentation					265:277	ethanol sedimentation	257:277	ethanol sedimentation	257:277	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	9	53	with	by-product	1665:1674	arg1	biomass					1686:1692	huge biomass	1681:1692	huge biomass	1681:1692	PRACTICAL APPLICATIONS: The leaf of Dendrobium officinale is a by-product with huge biomass.					
34873736	9	54	theme	Dendrobium	1638:1647	arg1	officinale					1649:1658	Dendrobium officinale	1638:1658	Dendrobium officinale	1638:1658	PRACTICAL APPLICATIONS: The leaf of Dendrobium officinale is a by-product with huge biomass.					
34873736	4	55	theme	scanning	736:743	arg1	microscope					754:763	The scanning electron microscope	732:763	The scanning electron microscope image of DLP-1	732:778	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	6	56	theme	magnetic	1037:1044	arg1	analysis					1056:1063	Nuclear magnetic resonance analysis	1029:1063	Nuclear magnetic resonance analysis	1029:1063	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	10	57	theme	research	1718:1725	arg1	effect					1773:1778	pharmacologic effect	1759:1778	pharmacologic effect	1759:1778	The lack of systematic research on its chemical composition and pharmacologic effect, leading to a great waste of resources.					
34873736	10	57	theme	research	1718:1725	arg1	lack					1699:1702	The lack	1695:1702	The lack of systematic research on its chemical composition	1695:1753	The lack of systematic research on its chemical composition and pharmacologic effect, leading to a great waste of resources.					
34873736	2	58	theme	1.93 × 106	574:583	arg1	Da					585:586	Mw 1.93 × 106 Da	571:586	Mw 1.93 × 106 Da	571:586	They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP-1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP-2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%).					
34873736	2	58	theme	1.93 × 106	574:583	arg1	DLP-2					564:568	DLP-2	564:568	DLP-2 (Mw 1.93 × 106 Da)	564:587	They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP-1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP-2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%).					
34873736	11	59	theme	D.	2065:2066	arg1	officinale					2068:2077	D. officinale	2065:2077	D. officinale	2065:2077	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	10	60	theme	pharmacologic	1759:1771	arg1	effect					1773:1778	pharmacologic effect	1759:1778	pharmacologic effect	1759:1778	The lack of systematic research on its chemical composition and pharmacologic effect, leading to a great waste of resources.					
34873736	11	61	from	DLP-2	2056:2060	arg1	officinale					2068:2077	D. officinale	2065:2077	D. officinale	2065:2077	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	8	62	theme	immunoregulatory	1524:1539	arg1	bioactivities					1541:1553	excellent immunoregulatory bioactivities	1514:1553	excellent immunoregulatory bioactivities	1514:1553	These results proved that both DLP-1 and DLP-2 possessed excellent immunoregulatory bioactivities and could be functional food or adjuvant drug.					
34873736	7	63	theme	nitric	1410:1415	arg1	oxide					1417:1421	nitric oxide	1410:1421	nitric oxide	1410:1421	Besides, DLP-1 and DLP-2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin-6, TNF-α, and IL-1β.					
34873736	0	64	from	officinale	93:102	arg1	polysaccharides					61:75	polysaccharides	61:75	polysaccharides from Dendrobium officinale	61:102	Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves.					
34873736	0	64	from	officinale	93:102	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves.					
34873736	0	64	from	officinale	93:102	arg1	activity					49:56	immunoregulatory activity	32:56	immunoregulatory activity	32:56	Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves.					
34873736	11	65	dep	characteristics	1916:1930	arg1	the					1901:1903	the	1901:1903	the	1901:1903	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	6	66	theme	linkage	1153:1159	arg1	-β-D-Glcp					1098:1106	(1→4)-β-D-Glcp	1093:1106	(1→4)-β-D-Glcp	1093:1106	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	66	theme	linkage	1153:1159	arg1	-β-D-Manp					1082:1090	(1→4)-β-D-Manp	1077:1090	(1→4)-β-D-Manp	1077:1090	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	66	theme	linkage	1153:1159	arg1	types					1161:1165	the main linkage types	1144:1165	the main linkage types of DLP-1	1144:1174	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	66	theme	linkage	1153:1159	arg1	-2-O-acetyl-β-D-Manp					1118:1137	(1→4)-2-O-acetyl-β-D-Manp	1113:1137	(1→4)-2-O-acetyl-β-D-Manp	1113:1137	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	4	67	theme	irregular	790:798	arg1	shape					819:823	an irregular and large lamellar shape	787:823	an irregular and large lamellar shape	787:823	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	4	68	theme	large	804:808	arg1	shape					819:823	an irregular and large lamellar shape	787:823	an irregular and large lamellar shape	787:823	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	11	69	from	DLP-1	2046:2050	arg1	officinale					2068:2077	D. officinale	2065:2077	D. officinale	2065:2077	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	7	70	theme	cells	1373:1377	arg1	proliferation					1320:1332	proliferation	1320:1332	proliferation	1320:1332	Besides, DLP-1 and DLP-2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin-6, TNF-α, and IL-1β.					
34873736	7	70	theme	cells	1373:1377	arg1	capacities					1349:1358	phagocytic capacities	1338:1358	phagocytic capacities	1338:1358	Besides, DLP-1 and DLP-2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin-6, TNF-α, and IL-1β.					
34873736	6	71	dep	-β-D-Manp	1082:1090	arg1	1→4					1078:1080	1→4	1078:1080	1→4	1078:1080	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	10	72	theme	great	1794:1798	arg1	waste					1800:1804	a great waste	1792:1804	a great waste of resources	1792:1817	The lack of systematic research on its chemical composition and pharmacologic effect, leading to a great waste of resources.					
34873736	6	73	theme	large	1212:1216	arg1	-β-D-Glcp					1249:1257	(1→4)-β-D-Glcp	1244:1257	(1→4)-β-D-Glcp	1244:1257	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	73	theme	large	1212:1216	arg1	-β-D-Manp					1233:1241	(1→4)-β-D-Manp	1228:1241	(1→4)-β-D-Manp	1228:1241	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	73	theme	large	1212:1216	arg1	amount					1218:1223	a large amount	1210:1223	a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues	1210:1277	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	73	theme	large	1212:1216	arg1	residues					1270:1277	other residues	1264:1277	other residues	1264:1277	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	1	74	theme	G-100	348:352	arg1	columns					354:360	DEAE-52 cellulose and Sephadex G-100 columns	317:360	columns	354:360	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	8	75	theme	adjuvant	1587:1594	arg1	drug					1596:1599	adjuvant drug	1587:1599	adjuvant drug	1587:1599	These results proved that both DLP-1 and DLP-2 possessed excellent immunoregulatory bioactivities and could be functional food or adjuvant drug.					
34873736	7	76	theme	RAW	1363:1365	arg1	cells					1373:1377	RAW 264.7 cells	1363:1377	RAW 264.7 cells	1363:1377	Besides, DLP-1 and DLP-2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin-6, TNF-α, and IL-1β.					
34873736	4	77	theme	porous	860:865	arg1	interior					867:874	a porous interior	858:874	a porous interior	858:874	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	0	78	from	characterization	11:26	arg1	officinale					93:102	Dendrobium officinale	82:102	Dendrobium officinale	82:102	Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves.					
34873736	5	79	theme	large	1000:1004	arg1	particles					1018:1026	large sponge-like particles	1000:1026	large sponge-like particles	1000:1026	In contrast, DLP-2 revealed a rough, loose, and uneven surface consisting of large sponge-like particles.					
34873736	10	80	from	effect	1773:1778	arg1	composition					1743:1753	its chemical composition	1730:1753	its chemical composition	1730:1753	The lack of systematic research on its chemical composition and pharmacologic effect, leading to a great waste of resources.					
34873736	1	81	from	leaves	161:166	arg1	DLP-2					211:215	DLP-2	211:215	DLP-2	211:215	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	1	81	from	leaves	161:166	arg1	polysaccharides					140:154	polysaccharides	140:154	polysaccharides from leaves of Dendrobium officinale	140:191	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	1	81	from	leaves	161:166	arg1	kinds					131:135	two kinds	127:135	two kinds of polysaccharides from leaves of Dendrobium officinale	127:191	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	1	81	from	leaves	161:166	arg1	DLP-1					201:205	DLP-1	201:205	DLP-1	201:205	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	4	82	theme	smooth	839:844	arg1	surface					846:852	a smooth surface	837:852	a smooth surface	837:852	The scanning electron microscope image of DLP-1 showed an irregular and large lamellar shape, as well as a smooth surface and a porous interior, illustrating they had an amorphous structure.					
34873736	11	83	dep	fractions	1978:1986	arg1	fractions					1978:1986	two polysaccharide fractions	1959:1986	two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves	1959:2030	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	11	83	dep	fractions	1978:1986	arg1	DLP-2					1999:2003	DLP-2	1999:2003	DLP-2	1999:2003	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	11	83	dep	fractions	1978:1986	arg1	DLP-1					1989:1993	DLP-1	1989:1993	DLP-1	1989:1993	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	5	84	theme	uneven	971:976	arg1	surface					978:984	a rough, loose, and uneven surface	951:984	a rough, loose, and uneven surface consisting of large sponge-like particles	951:1026	In contrast, DLP-2 revealed a rough, loose, and uneven surface consisting of large sponge-like particles.					
34873736	9	85	theme	PRACTICAL	1602:1610	arg1	APPLICATIONS					1612:1623	PRACTICAL APPLICATIONS	1602:1623	PRACTICAL APPLICATIONS: The leaf of Dendrobium officinale is a by-product with huge biomass.	1602:1693	PRACTICAL APPLICATIONS: The leaf of Dendrobium officinale is a by-product with huge biomass.					
34873736	2	86	theme	different	385:393	arg1	monosaccharides					395:409	different monosaccharides	385:409	different monosaccharides	385:409	They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP-1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP-2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%).					
34873736	0	87	theme	immunoregulatory	32:47	arg1	activity					49:56	immunoregulatory activity	32:56	immunoregulatory activity	32:56	Structural characterization and immunoregulatory activity of polysaccharides from Dendrobium officinale leaves.					
34873736	1	88	from	kinds	131:135	arg1	leaves					161:166	leaves	161:166	leaves	161:166	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	2	89	theme	monosaccharides	430:444	arg1	content					419:425	the content	415:425	the content of monosaccharides	415:444	They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP-1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP-2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%).					
34873736	6	90	theme	-β-D-Manp	1233:1241	arg1	-β-D-Glcp					1249:1257	(1→4)-β-D-Glcp	1244:1257	(1→4)-β-D-Glcp	1244:1257	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	90	theme	-β-D-Manp	1233:1241	arg1	-β-D-Manp					1233:1241	(1→4)-β-D-Manp	1228:1241	(1→4)-β-D-Manp	1228:1241	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	90	theme	-β-D-Manp	1233:1241	arg1	amount					1218:1223	a large amount	1210:1223	a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues	1210:1277	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	90	theme	-β-D-Manp	1233:1241	arg1	residues					1270:1277	other residues	1264:1277	other residues	1264:1277	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	6	91	dep	-β-D-Manp	1233:1241	arg1	1→4					1229:1231	1→4	1229:1231	1→4	1229:1231	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	7	92	theme	phagocytic	1338:1347	arg1	capacities					1349:1358	phagocytic capacities	1338:1358	phagocytic capacities	1338:1358	Besides, DLP-1 and DLP-2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin-6, TNF-α, and IL-1β.					
34873736	1	93	theme	DEAE-52	317:323	arg1	cellulose					325:333	DEAE-52 cellulose and Sephadex G-100 columns	317:360	cellulose	325:333	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
34873736	6	94	dep	-β-D-Glcp	1249:1257	arg1	1→4					1245:1247	1→4	1245:1247	1→4	1245:1247	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	2	95	theme	Mw	480:481	arg1	DLP-1					473:477	DLP-1	473:477	DLP-1 (Mw 1.38 × 106 Da)	473:496	They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP-1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP-2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%).					
34873736	2	95	theme	Mw	480:481	arg1	Da					494:495	Mw 1.38 × 106 Da	480:495	Mw 1.38 × 106 Da	480:495	They were composed of different monosaccharides and the content of monosaccharides varied significantly while DLP-1 (Mw 1.38 × 106 Da) was mainly composed of mannose (71.69%) and glucose (22.89%), and DLP-2 (Mw 1.93 × 106 Da) was constituted by rhamnose (35.05%), arabinose (24.12%), and galactose (25.65%).					
34873736	11	96	dep	officinale	2014:2023	arg1	leaves					2025:2030	leaves	2025:2030	leaves	2025:2030	In order to maximize the value of D. officinale, this study aimed to investigate the structural characteristics and immunologic effects of two polysaccharide fractions (DLP-1 and DLP-2) from D. officinale leaves, showing that DLP-1 and DLP-2 in D. officinale leaves could be used as anti-inflammatory agents to avoid wasting.					
34873736	7	97	dep	proliferation	1320:1332	arg1	the					1316:1318	the	1316:1318	the	1316:1318	Besides, DLP-1 and DLP-2 stimulated the proliferation and phagocytic capacities of RAW 264.7 cells and improved the production of nitric oxide, interleukin-6, TNF-α, and IL-1β.					
34873736	6	98	dep	-β-D-Glcp	1098:1106	arg1	1→4					1094:1096	1→4	1094:1096	1→4	1094:1096	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	10	99	theme	systematic	1707:1716	arg1	research					1718:1725	systematic research	1707:1725	systematic research	1707:1725	The lack of systematic research on its chemical composition and pharmacologic effect, leading to a great waste of resources.					
34873736	6	100	theme	other	1264:1268	arg1	residues					1270:1277	other residues	1264:1277	other residues	1264:1277	Nuclear magnetic resonance analysis showed that (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and (1→4)-2-O-acetyl-β-D-Manp were the main linkage types of DLP-1, whereas DLP-2 was constituted by a large amount of (1→4)-β-D-Manp, (1→4)-β-D-Glcp, and other residues.					
34873736	1	101	theme	water	239:243	arg1	extraction					245:254	hot water extraction	235:254	hot water extraction	235:254	In this study, two kinds of polysaccharides from leaves of Dendrobium officinale, namely DLP-1 and DLP-2, were obtained by hot water extraction, ethanol sedimentation, and chromatographic separation using DEAE-52 cellulose and Sephadex G-100 columns.					
32227698	7	0	theme	encapsulation	1418:1430	arg1	efficiency					1432:1441	high encapsulation efficiency	1413:1441	high encapsulation efficiency	1413:1441	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	7	0	theme	encapsulation	1418:1430	arg1	yields					1405:1410	high yields	1400:1410	high yields	1400:1410	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	8	1	theme	regular	1557:1563	arg1	shape					1565:1569	the more regular shape	1548:1569	the more regular shape of the microcapsules	1548:1590	The spray drying process can offer better applications for the food industry due to the more regular shape of the microcapsules.					
32227698	5	2	theme	electron	1102:1109	arg1	microscopy					1111:1120	scanning electron microscopy	1093:1120	scanning electron microscopy	1093:1120	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	10	3	theme	different	2043:2051	arg1	compositions					2053:2064	different compositions	2043:2064	different compositions	2043:2064	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	10	4	theme	non-volatile	1906:1917	arg1	compounds					1919:1927	non-volatile compounds	1906:1927	non-volatile compounds from oleoresins	1906:1943	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	6	5	theme	Folin-Ciocalteau	1178:1193	arg1	method					1195:1200	the Folin-Ciocalteau method	1174:1200	the Folin-Ciocalteau method	1174:1200	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	7	6	theme	high	1400:1403	arg1	efficiency					1432:1441	high encapsulation efficiency	1413:1441	high encapsulation efficiency	1413:1441	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	7	6	theme	high	1400:1403	arg1	solubility					1452:1461	good solubility	1447:1461	good solubility	1447:1461	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	7	6	theme	high	1400:1403	arg1	yields					1405:1410	high yields	1400:1410	high yields	1400:1410	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	5	7	dep	methods	937:943	arg1	efficiency					1026:1035	encapsulation efficiency	1012:1035	encapsulation efficiency	1012:1035	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	7	dep	methods	937:943	arg1	yields					953:958	drying yields	946:958	drying yields	946:958	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	7	dep	methods	937:943	arg1	hygroscopicity					989:1002	hygroscopicity	989:1002	hygroscopicity	989:1002	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	7	dep	methods	937:943	arg1	color					1005:1009	color	1005:1009	color	1005:1009	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	7	dep	methods	937:943	arg1	activity					967:974	water activity	961:974	water activity	961:974	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	7	dep	methods	937:943	arg1	solubility					977:986	solubility	977:986	solubility	977:986	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	2	8	with	encapsulation	357:369	arg1	maltodextrin					387:398	maltodextrin	387:398	maltodextrin	387:398	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	2	8	with	encapsulation	357:369	arg1	inulin					376:381	inulin	376:381	inulin	376:381	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	9	9	theme	inulin	1606:1611	arg1	microcapsules					1613:1625	inulin microcapsules	1606:1625	inulin microcapsules obtained by spray drying	1606:1650	In addition, inulin microcapsules obtained by spray drying showed a more protective effect for flavonoid compounds in fish oleoresins, while maltodextrin microcapsules offered more protection for hydroxycinnamic acids in meat oleoresins.					
32227698	7	10	theme	spray	1332:1336	arg1	drying					1338:1343	spray drying	1332:1343	spray drying	1332:1343	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	7	10	theme	spray	1332:1336	arg1	RESULTS					1313:1319	RESULTS Freeze and spray drying	1313:1343	RESULTS Freeze and spray drying the microcapsules	1313:1361	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	7	11	dep	RESULTS	1313:1319	arg1	microcapsules					1349:1361	the microcapsules	1345:1361	RESULTS Freeze and spray drying the microcapsules	1313:1361	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	7	11	dep	RESULTS	1313:1319	arg1	Freeze					1321:1326	Freeze	1321:1326	Freeze	1321:1326	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	7	11	dep	RESULTS	1313:1319	arg1	drying					1338:1343	spray drying	1332:1343	spray drying	1332:1343	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	7	11	dep	RESULTS	1313:1319	arg1	RESULTS					1313:1319	RESULTS Freeze and spray drying	1313:1343	RESULTS Freeze and spray drying the microcapsules	1313:1361	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	9	12	from	acids	1805:1809	arg1	oleoresins					1819:1828	meat oleoresins	1814:1828	meat oleoresins	1814:1828	In addition, inulin microcapsules obtained by spray drying showed a more protective effect for flavonoid compounds in fish oleoresins, while maltodextrin microcapsules offered more protection for hydroxycinnamic acids in meat oleoresins.					
32227698	9	13	theme	spray	1639:1643	arg1	drying					1645:1650	spray drying	1639:1650	spray drying	1639:1650	In addition, inulin microcapsules obtained by spray drying showed a more protective effect for flavonoid compounds in fish oleoresins, while maltodextrin microcapsules offered more protection for hydroxycinnamic acids in meat oleoresins.					
32227698	10	14	theme	standardized	1963:1974	arg1	taste					1976:1980	standardized taste and aroma products	1963:1999	taste	1976:1980	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	10	15	with	systems	2030:2036	arg1	compositions					2053:2064	different compositions	2043:2064	different compositions	2043:2064	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	6	16	theme	total	1127:1131	arg1	compounds					1142:1150	The total phenolic compounds	1123:1150	The total phenolic compounds	1123:1150	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	2	17	from	salt	555:558	arg1	industry					588:595	the food industry	579:595	the food industry	579:595	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	5	18	theme	drying	946:951	arg1	efficiency					1026:1035	encapsulation efficiency	1012:1035	encapsulation efficiency	1012:1035	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	18	theme	drying	946:951	arg1	yields					953:958	drying yields	946:958	drying yields	946:958	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	18	theme	drying	946:951	arg1	hygroscopicity					989:1002	hygroscopicity	989:1002	hygroscopicity	989:1002	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	18	theme	drying	946:951	arg1	color					1005:1009	color	1005:1009	color	1005:1009	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	18	theme	drying	946:951	arg1	activity					967:974	water activity	961:974	water activity	961:974	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	18	theme	drying	946:951	arg1	solubility					977:986	solubility	977:986	solubility	977:986	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	19	theme	encapsulation	1012:1024	arg1	efficiency					1026:1035	encapsulation efficiency	1012:1035	encapsulation efficiency	1012:1035	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	19	theme	encapsulation	1012:1024	arg1	yields					953:958	drying yields	946:958	drying yields	946:958	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	9	20	theme	flavonoid	1688:1696	arg1	compounds					1698:1706	flavonoid compounds	1688:1706	flavonoid compounds	1688:1706	In addition, inulin microcapsules obtained by spray drying showed a more protective effect for flavonoid compounds in fish oleoresins, while maltodextrin microcapsules offered more protection for hydroxycinnamic acids in meat oleoresins.					
32227698	9	21	theme	protective	1666:1675	arg1	effect					1677:1682	a more protective effect	1659:1682	a more protective effect for flavonoid compounds	1659:1706	In addition, inulin microcapsules obtained by spray drying showed a more protective effect for flavonoid compounds in fish oleoresins, while maltodextrin microcapsules offered more protection for hydroxycinnamic acids in meat oleoresins.					
32227698	6	22	theme	mass	1294:1297	arg1	spectrometry					1299:1310	high resolution mass spectrometry	1278:1310	high resolution mass spectrometry	1278:1310	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	1	23	theme	original	139:146	arg1	spice					155:159	the original ground spice	135:159	the original ground spice	135:159	BACKGROUND In our study, oleoresins were explored for their ability to replace the original ground spice and herbs with a standardized taste and aroma, and mask the salt reduction.					
32227698	6	24	theme	high	1278:1281	arg1	spectrometry					1299:1310	high resolution mass spectrometry	1278:1310	high resolution mass spectrometry	1278:1310	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	10	25	from	oleoresins	1934:1943	arg1	compounds					1919:1927	non-volatile compounds	1906:1927	non-volatile compounds from oleoresins	1906:1943	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	2	26	theme	drying	458:463	arg1	spray					476:480	spray	476:480	spray	476:480	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	2	26	theme	drying	458:463	arg1	drying					493:498	freeze drying	486:498	freeze drying	486:498	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	2	26	theme	drying	458:463	arg1	processes					465:473	two drying processes	454:473	two drying processes (spray and freeze drying)	454:499	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	2	27	theme	food	342:345	arg1	matrices					347:354	food matrices	342:354	food matrices	342:354	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	5	28	theme	Physicochemical	881:895	arg1	analyses					897:904	Physicochemical analyses	881:904	Physicochemical analyses	881:904	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	8	29	theme	spray	1468:1472	arg1	process					1481:1487	The spray drying process	1464:1487	The spray drying process	1464:1487	The spray drying process can offer better applications for the food industry due to the more regular shape of the microcapsules.					
32227698	2	30	theme	food	583:586	arg1	industry					588:595	the food industry	579:595	the food industry	579:595	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	5	31	theme	microcapsules	1067:1079	arg1	characterization					1043:1058	characterization	1043:1058	characterization of the microcapsules	1043:1079	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	0	32	theme	oleoresins	17:26	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of oleoresins for salt reduction in food.	0:54	Encapsulation of oleoresins for salt reduction in food.					
32227698	9	33	theme	more	1769:1772	arg1	protection					1774:1783	more protection	1769:1783	more protection	1769:1783	In addition, inulin microcapsules obtained by spray drying showed a more protective effect for flavonoid compounds in fish oleoresins, while maltodextrin microcapsules offered more protection for hydroxycinnamic acids in meat oleoresins.					
32227698	4	34	theme	solvent	719:725	arg1	extraction					727:736	solvent extraction	719:736	solvent extraction	719:736	Oleoresins were obtained by solvent extraction, and the solvent was removed by evaporation, encapsulated into inulin and maltodextrin microcapsules, and powdered by spray and freeze drying.					
32227698	0	35	theme	salt	32:35	arg1	reduction					37:45	salt reduction	32:45	salt reduction in food	32:53	Encapsulation of oleoresins for salt reduction in food.					
32227698	2	36	dep	processes	465:473	arg1	spray					476:480	spray	476:480	spray	476:480	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	2	36	dep	processes	465:473	arg1	drying					493:498	freeze drying	486:498	freeze drying	486:498	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	2	36	dep	processes	465:473	arg1	processes					465:473	two drying processes	454:473	two drying processes (spray and freeze drying)	454:499	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	5	37	theme	several	929:935	arg1	methods					937:943	several methods	929:943	several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency)	929:1036	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	2	38	from	solubility	328:337	arg1	matrices					347:354	food matrices	342:354	food matrices	342:354	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	2	39	theme	oleoresins	437:446	arg1	mixtures					425:432	two mixtures	421:432	two mixtures of oleoresins using two drying processes (spray and freeze drying)	421:499	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	6	40	from	present	1230:1236	arg1	microcapsules					1245:1257	the microcapsules	1241:1257	the microcapsules	1241:1257	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	1	41	with	herbs	165:169	arg1	aroma					201:205	aroma	201:205	aroma	201:205	BACKGROUND In our study, oleoresins were explored for their ability to replace the original ground spice and herbs with a standardized taste and aroma, and mask the salt reduction.					
32227698	1	41	with	herbs	165:169	arg1	taste					191:195	a standardized taste	176:195	a standardized taste	176:195	BACKGROUND In our study, oleoresins were explored for their ability to replace the original ground spice and herbs with a standardized taste and aroma, and mask the salt reduction.					
32227698	7	42	theme	good	1447:1450	arg1	solubility					1452:1461	good solubility	1447:1461	good solubility	1447:1461	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	7	42	theme	good	1447:1450	arg1	yields					1405:1410	high yields	1400:1410	high yields	1400:1410	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	1	43	with	spice	155:159	arg1	aroma					201:205	aroma	201:205	aroma	201:205	BACKGROUND In our study, oleoresins were explored for their ability to replace the original ground spice and herbs with a standardized taste and aroma, and mask the salt reduction.					
32227698	1	43	with	spice	155:159	arg1	taste					191:195	a standardized taste	176:195	a standardized taste	176:195	BACKGROUND In our study, oleoresins were explored for their ability to replace the original ground spice and herbs with a standardized taste and aroma, and mask the salt reduction.					
32227698	10	44	theme	food	2025:2028	arg1	systems					2030:2036	food systems	2025:2036	food systems with different compositions	2025:2064	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	7	45	theme	high	1413:1416	arg1	efficiency					1432:1441	high encapsulation efficiency	1413:1441	high encapsulation efficiency	1413:1441	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	7	45	theme	high	1413:1416	arg1	yields					1405:1410	high yields	1400:1410	high yields	1400:1410	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	3	46	theme	oleoresins	610:619	arg1	experiment					621:630	The oleoresins experiment	606:630	The oleoresins experiment	606:630	METHODS The oleoresins experiment was conducted with two mixtures to apply to meat and fish.					
32227698	5	47	theme	scanning	1093:1100	arg1	microscopy					1111:1120	scanning electron microscopy	1093:1120	scanning electron microscopy	1093:1120	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	1	48	theme	salt	221:224	arg1	reduction					226:234	the salt reduction	217:234	the salt reduction	217:234	BACKGROUND In our study, oleoresins were explored for their ability to replace the original ground spice and herbs with a standardized taste and aroma, and mask the salt reduction.					
32227698	7	49	theme	good	1373:1376	arg1	products					1386:1393	good quality products	1373:1393	good quality products	1373:1393	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	1	50	theme	ground	148:153	arg1	spice					155:159	the original ground spice	135:159	the original ground spice	135:159	BACKGROUND In our study, oleoresins were explored for their ability to replace the original ground spice and herbs with a standardized taste and aroma, and mask the salt reduction.					
32227698	7	51	theme	quality	1378:1384	arg1	products					1386:1393	good quality products	1373:1393	good quality products	1373:1393	RESULTS Freeze and spray drying the microcapsules presented good quality products with high yields, high encapsulation efficiency and good solubility.					
32227698	10	52	theme	encapsulation	1881:1893	arg1	system					1895:1900	an attractive encapsulation system	1867:1900	an attractive encapsulation system	1867:1900	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	2	53	theme	aroma	267:271	arg1	oleoresins					273:282	taste and aroma oleoresins	257:282	oleoresins	273:282	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	2	54	theme	taste	257:261	arg1	oleoresins					273:282	taste and aroma oleoresins	257:282	oleoresins	273:282	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	4	55	theme	maltodextrin	812:823	arg1	microcapsules					825:837	inulin and maltodextrin microcapsules	801:837	microcapsules	825:837	Oleoresins were obtained by solvent extraction, and the solvent was removed by evaporation, encapsulated into inulin and maltodextrin microcapsules, and powdered by spray and freeze drying.					
32227698	1	56	theme	standardized	178:189	arg1	taste					191:195	a standardized taste	176:195	a standardized taste	176:195	BACKGROUND In our study, oleoresins were explored for their ability to replace the original ground spice and herbs with a standardized taste and aroma, and mask the salt reduction.					
32227698	6	57	theme	phenolic	1133:1140	arg1	compounds					1142:1150	The total phenolic compounds	1123:1150	The total phenolic compounds	1123:1150	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	6	58	theme	present	1230:1236	arg1	compounds					1220:1228	the chemical compounds	1207:1228	the chemical compounds present in the microcapsules	1207:1257	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	2	59	used	used	525:528	arg2	it					516:517	it	516:517	it	516:517	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	2	59	used	used	525:528	arg2	additive					536:543	additive	536:543	additive	536:543	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	6	60	theme	chemical	1211:1218	arg1	compounds					1220:1228	the chemical compounds	1207:1228	the chemical compounds present in the microcapsules	1207:1257	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	4	61	theme	inulin	801:806	arg1	microcapsules					825:837	inulin and maltodextrin microcapsules	801:837	microcapsules	825:837	Oleoresins were obtained by solvent extraction, and the solvent was removed by evaporation, encapsulated into inulin and maltodextrin microcapsules, and powdered by spray and freeze drying.					
32227698	1	62	dep	BACKGROUND	56:65	arg1	explored					97:104	explored	97:104	were explored for their ability to replace the original ground spice and herbs with a standardized taste and aroma, and mask the salt reduction	92:234	BACKGROUND In our study, oleoresins were explored for their ability to replace the original ground spice and herbs with a standardized taste and aroma, and mask the salt reduction.					
32227698	10	63	theme	aroma	1986:1990	arg1	products					1992:1999	standardized taste and aroma products	1963:1999	products	1992:1999	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	5	64	theme	water	961:965	arg1	yields					953:958	drying yields	946:958	drying yields	946:958	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	5	64	theme	water	961:965	arg1	activity					967:974	water activity	961:974	water activity	961:974	Physicochemical analyses were carried out using several methods (drying yields, water activity, solubility, hygroscopicity, color, encapsulation efficiency), and characterization of the microcapsules was done by scanning electron microscopy.					
32227698	4	65	theme	freeze	866:871	arg1	drying					873:878	freeze drying	866:878	freeze drying	866:878	Oleoresins were obtained by solvent extraction, and the solvent was removed by evaporation, encapsulated into inulin and maltodextrin microcapsules, and powdered by spray and freeze drying.					
32227698	10	66	dep	CONCLUSIONS	1831:1841	arg1	shows					1861:1865	shows	1861:1865	shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions	1861:2064	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	2	67	theme	freeze	486:491	arg1	drying					493:498	freeze drying	486:498	freeze drying	486:498	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	2	67	theme	freeze	486:491	arg1	processes					465:473	two drying processes	454:473	two drying processes (spray and freeze drying)	454:499	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	6	68	theme	resolution	1283:1292	arg1	spectrometry					1299:1310	high resolution mass spectrometry	1278:1310	high resolution mass spectrometry	1278:1310	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	9	69	theme	fish	1711:1714	arg1	oleoresins					1716:1725	fish oleoresins	1711:1725	fish oleoresins	1711:1725	In addition, inulin microcapsules obtained by spray drying showed a more protective effect for flavonoid compounds in fish oleoresins, while maltodextrin microcapsules offered more protection for hydroxycinnamic acids in meat oleoresins.					
32227698	9	70	theme	meat	1814:1817	arg1	oleoresins					1819:1828	meat oleoresins	1814:1828	meat oleoresins	1814:1828	In addition, inulin microcapsules obtained by spray drying showed a more protective effect for flavonoid compounds in fish oleoresins, while maltodextrin microcapsules offered more protection for hydroxycinnamic acids in meat oleoresins.					
32227698	0	71	from	reduction	37:45	arg1	food					50:53	food	50:53	food	50:53	Encapsulation of oleoresins for salt reduction in food.					
32227698	9	72	theme	maltodextrin	1734:1745	arg1	microcapsules					1747:1759	maltodextrin microcapsules	1734:1759	maltodextrin microcapsules	1734:1759	In addition, inulin microcapsules obtained by spray drying showed a more protective effect for flavonoid compounds in fish oleoresins, while maltodextrin microcapsules offered more protection for hydroxycinnamic acids in meat oleoresins.					
32227698	3	73	dep	METHODS	598:604	arg1	conducted					636:644	conducted	636:644	was conducted with two mixtures to apply to meat and fish	632:688	METHODS The oleoresins experiment was conducted with two mixtures to apply to meat and fish.					
32227698	8	74	theme	better	1499:1504	arg1	applications					1506:1517	better applications	1499:1517	better applications	1499:1517	The spray drying process can offer better applications for the food industry due to the more regular shape of the microcapsules.					
32227698	6	75	from	microcapsules	1245:1257	arg1	present					1230:1236	present	1230:1236	present	1230:1236	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	8	76	theme	drying	1474:1479	arg1	process					1481:1487	The spray drying process	1464:1487	The spray drying process	1464:1487	The spray drying process can offer better applications for the food industry due to the more regular shape of the microcapsules.					
32227698	9	77	theme	hydroxycinnamic	1789:1803	arg1	acids					1805:1809	hydroxycinnamic acids	1789:1809	hydroxycinnamic acids in meat oleoresins	1789:1828	In addition, inulin microcapsules obtained by spray drying showed a more protective effect for flavonoid compounds in fish oleoresins, while maltodextrin microcapsules offered more protection for hydroxycinnamic acids in meat oleoresins.					
32227698	8	78	theme	food	1527:1530	arg1	industry					1532:1539	the food industry	1523:1539	the food industry due to the more regular shape of the microcapsules	1523:1590	The spray drying process can offer better applications for the food industry due to the more regular shape of the microcapsules.					
32227698	10	79	theme	present	1847:1853	arg1	study					1855:1859	The present study	1843:1859	The present study	1843:1859	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	2	80	theme	high	289:292	arg1	temperature					294:304	high temperature	289:304	high temperature	289:304	In order to protect taste and aroma oleoresins from high temperature, and to improve their solubility in food matrices, encapsulation with inulin and maltodextrin was carried out from two mixtures of oleoresins using two drying processes (spray and freeze drying), thus allowing it to be used as an additive to reduce salt for convenience in the food industry.					
32227698	8	81	theme	microcapsules	1578:1590	arg1	shape					1565:1569	the more regular shape	1548:1569	the more regular shape of the microcapsules	1548:1590	The spray drying process can offer better applications for the food industry due to the more regular shape of the microcapsules.					
32227698	10	82	from	salt	2017:2020	arg1	systems					2030:2036	food systems	2025:2036	food systems with different compositions	2025:2064	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
32227698	6	83	attach	present	1230:1236	arg1	microcapsules					1245:1257	the microcapsules	1241:1257	the microcapsules	1241:1257	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	6	83	attach	present	1230:1236	arg2	compounds					1220:1228	the chemical compounds	1207:1228	the chemical compounds present in the microcapsules	1207:1257	The total phenolic compounds were quantified using the Folin-Ciocalteau method, and the chemical compounds present in the microcapsules were elucidated by high resolution mass spectrometry.					
32227698	8	84	theme	due	1541:1543	arg1	industry					1532:1539	the food industry	1523:1539	the food industry due to the more regular shape of the microcapsules	1523:1590	The spray drying process can offer better applications for the food industry due to the more regular shape of the microcapsules.					
32227698	10	85	theme	attractive	1870:1879	arg1	system					1895:1900	an attractive encapsulation system	1867:1900	an attractive encapsulation system	1867:1900	CONCLUSIONS The present study shows an attractive encapsulation system for non-volatile compounds from oleoresins, which results in standardized taste and aroma products that can reduce salt in food systems with different compositions.					
33065002	5	0	from	subunits	787:794	arg1	c-ring					821:826	the c-ring	817:826	the c-ring	817:826	We define ATP6AP1 as a structural hub for Vo complex assembly because it connects to multiple Vo subunits and phospholipids in the c-ring.					
33065002	3	1	from	structures	448:457	arg1	states					497:502	three rotational states	480:502	three rotational states at up to 2.9-Å resolution	480:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	1	2	theme	proton	156:161	arg1	triphosphatases					113:127	vacuolar-type adenosine triphosphatases	89:127	vacuolar-type adenosine triphosphatases (V-ATPases)	89:139	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	2	theme	proton	156:161	arg1	Vesicular-					75:84	Vesicular-	75:84	Vesicular-	75:84	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	2	theme	proton	156:161	arg1	pumps					163:167	ATP-driven proton pumps	145:167	ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer	145:279	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	5	3	theme	complex	735:741	arg1	assembly					743:750	Vo complex assembly	732:750	Vo complex assembly	732:750	We define ATP6AP1 as a structural hub for Vo complex assembly because it connects to multiple Vo subunits and phospholipids in the c-ring.					
33065002	1	4	theme	Vo	250:251	arg1	complex					253:259	a membrane-embedded Vo complex	230:259	a membrane-embedded Vo complex for proton transfer	230:279	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	0	5	theme	Assembly	65:72	arg1	Mechanisms					47:56	Mechanisms	47:56	Mechanisms of Its Assembly	47:72	Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly.					
33065002	2	6	theme	organelles	352:361	arg1	acidification					311:323	acidification	311:323	acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes	311:405	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	2	7	from	acidification	311:323	arg1	eukaryotes					396:405	eukaryotes	396:405	eukaryotes	396:405	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	6	8	theme	glycan	893:898	arg1	coat					900:903	a luminal glycan coat	883:903	a luminal glycan coat critical for V-ATPase folding, localization, and stability	883:962	The glycolipids and the glycosylated Vo subunits form a luminal glycan coat critical for V-ATPase folding, localization, and stability.					
33065002	1	9	theme	adenosine	103:111	arg1	V-ATPases					130:138	V-ATPases	130:138	V-ATPases	130:138	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	9	theme	adenosine	103:111	arg1	triphosphatases					113:127	vacuolar-type adenosine triphosphatases	89:127	vacuolar-type adenosine triphosphatases (V-ATPases)	89:139	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	9	theme	adenosine	103:111	arg1	Vesicular-					75:84	Vesicular-	75:84	Vesicular-	75:84	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	9	theme	adenosine	103:111	arg1	pumps					163:167	ATP-driven proton pumps	145:167	ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer	145:279	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	6	10	theme	luminal	885:891	arg1	coat					900:903	a luminal glycan coat	883:903	a luminal glycan coat critical for V-ATPase folding, localization, and stability	883:962	The glycolipids and the glycosylated Vo subunits form a luminal glycan coat critical for V-ATPase folding, localization, and stability.					
33065002	7	11	theme	lipids	1093:1098	arg1	roles					1062:1066	the integrated roles	1047:1066	the integrated roles of ATP6AP1, glycans, and lipids	1047:1098	This study identifies mechanisms of V-ATPase assembly and biogenesis that rely on the integrated roles of ATP6AP1, glycans, and lipids.					
33065002	1	12	theme	proton	265:270	arg1	transfer					272:279	proton transfer	265:279	proton transfer	265:279	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	5	13	theme	Vo	732:733	arg1	assembly					743:750	Vo complex assembly	732:750	Vo complex assembly	732:750	We define ATP6AP1 as a structural hub for Vo complex assembly because it connects to multiple Vo subunits and phospholipids in the c-ring.					
33065002	7	14	theme	glycans	1080:1086	arg1	roles					1062:1066	the integrated roles	1047:1066	the integrated roles of ATP6AP1, glycans, and lipids	1047:1098	This study identifies mechanisms of V-ATPase assembly and biogenesis that rely on the integrated roles of ATP6AP1, glycans, and lipids.					
33065002	2	15	from	vesicles	342:349	arg1	eukaryotes					396:405	eukaryotes	396:405	eukaryotes	396:405	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	4	16	theme	Vo	678:679	arg1	complex					681:687	the Vo complex	674:687	the Vo complex	674:687	Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the Vo complex.					
33065002	3	17	theme	microscopy	437:446	arg1	structures					448:457	cryoelectron microscopy structures	424:457	cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution	424:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	4	18	theme	protein	578:584	arg1	subunits					586:593	all known protein subunits	568:593	all known protein subunits with associated N-linked glycans	568:626	Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the Vo complex.					
33065002	3	19	theme	cryoelectron	424:435	arg1	microscopy					437:446	cryoelectron microscopy	424:446	cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution	424:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	4	20	theme	known	572:576	arg1	subunits					586:593	all known protein subunits	568:593	all known protein subunits with associated N-linked glycans	568:626	Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the Vo complex.					
33065002	5	21	from	phospholipids	800:812	arg1	c-ring					821:826	the c-ring	817:826	the c-ring	817:826	We define ATP6AP1 as a structural hub for Vo complex assembly because it connects to multiple Vo subunits and phospholipids in the c-ring.					
33065002	7	22	theme	ATP6AP1	1071:1077	arg1	roles					1062:1066	the integrated roles	1047:1066	the integrated roles of ATP6AP1, glycans, and lipids	1047:1098	This study identifies mechanisms of V-ATPase assembly and biogenesis that rely on the integrated roles of ATP6AP1, glycans, and lipids.					
33065002	2	23	from	organelles	352:361	arg1	eukaryotes					396:405	eukaryotes	396:405	eukaryotes	396:405	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	4	24	from	phospholipids	657:669	arg1	complex					681:687	the Vo complex	674:687	the Vo complex	674:687	Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the Vo complex.					
33065002	2	25	theme	milieu	386:391	arg1	acidification					311:323	acidification	311:323	acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes	311:405	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	3	26	from	states	497:502	arg1	structures					448:457	cryoelectron microscopy structures	424:457	cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution	424:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	6	27	gly	glycosylated	853:864	arg1	subunits					869:876	the glycosylated Vo subunits	849:876	the glycosylated Vo subunits	849:876	The glycolipids and the glycosylated Vo subunits form a luminal glycan coat critical for V-ATPase folding, localization, and stability.					
33065002	2	28	theme	extracellular	372:384	arg1	milieu					386:391	the extracellular milieu	368:391	the extracellular milieu in eukaryotes	368:405	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	0	29	theme	Complete	16:23	arg1	V-ATPase					31:38	a Complete Human V-ATPase	14:38	a Complete Human V-ATPase	14:38	Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly.					
33065002	3	30	from	resolution	519:528	arg1	states					497:502	three rotational states	480:502	three rotational states at up to 2.9-Å resolution	480:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	3	30	from	resolution	519:528	arg1	V-ATPase					468:475	human V-ATPase	462:475	human V-ATPase in three rotational states at up to 2.9-Å resolution	462:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	1	31	theme	vacuolar-type	89:101	arg1	V-ATPases					130:138	V-ATPases	130:138	V-ATPases	130:138	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	31	theme	vacuolar-type	89:101	arg1	triphosphatases					113:127	vacuolar-type adenosine triphosphatases	89:127	vacuolar-type adenosine triphosphatases (V-ATPases)	89:139	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	31	theme	vacuolar-type	89:101	arg1	Vesicular-					75:84	Vesicular-	75:84	Vesicular-	75:84	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	31	theme	vacuolar-type	89:101	arg1	pumps					163:167	ATP-driven proton pumps	145:167	ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer	145:279	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	4	32	from	glycolipids	641:651	arg1	complex					681:687	the Vo complex	674:687	the Vo complex	674:687	Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the Vo complex.					
33065002	1	33	theme	V1	196:197	arg1	complex					199:205	a cytoplasmic V1 complex	182:205	a cytoplasmic V1 complex for ATP hydrolysis	182:224	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	3	34	from	V-ATPase	468:475	arg1	states					497:502	three rotational states	480:502	three rotational states at up to 2.9-Å resolution	480:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	3	34	from	V-ATPase	468:475	arg1	resolution					519:528	up to 2.9-Å resolution	507:528	up to 2.9-Å resolution	507:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	6	35	theme	Vo	866:867	arg1	subunits					869:876	the glycosylated Vo subunits	849:876	the glycosylated Vo subunits	849:876	The glycolipids and the glycosylated Vo subunits form a luminal glycan coat critical for V-ATPase folding, localization, and stability.					
33065002	2	36	theme	important	292:300	arg1	roles					302:306	important roles	292:306	important roles	292:306	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	6	37	theme	critical	905:912	arg1	coat					900:903	a luminal glycan coat	883:903	a luminal glycan coat critical for V-ATPase folding, localization, and stability	883:962	The glycolipids and the glycosylated Vo subunits form a luminal glycan coat critical for V-ATPase folding, localization, and stability.					
33065002	0	38	theme	V-ATPase	31:38	arg1	Structures					0:9	Structures	0:9	Structures of a Complete Human V-ATPase	0:38	Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly.					
33065002	4	39	link	N-linked	611:618	arg1	glycans					620:626	associated N-linked glycans	600:626	associated N-linked glycans	600:626	Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the Vo complex.					
33065002	1	40	theme	cytoplasmic	184:194	arg1	complex					199:205	a cytoplasmic V1 complex	182:205	a cytoplasmic V1 complex for ATP hydrolysis	182:224	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	0	41	theme	Human	25:29	arg1	V-ATPase					31:38	a Complete Human V-ATPase	14:38	a Complete Human V-ATPase	14:38	Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly.					
33065002	5	42	theme	multiple	775:782	arg1	subunits					787:794	multiple Vo subunits	775:794	multiple Vo subunits	775:794	We define ATP6AP1 as a structural hub for Vo complex assembly because it connects to multiple Vo subunits and phospholipids in the c-ring.					
33065002	2	43	from	milieu	386:391	arg1	eukaryotes					396:405	eukaryotes	396:405	eukaryotes	396:405	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	1	44	theme	ATP	211:213	arg1	hydrolysis					215:224	ATP hydrolysis	211:224	ATP hydrolysis	211:224	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	5	45	theme	Vo	784:785	arg1	subunits					787:794	multiple Vo subunits	775:794	multiple Vo subunits	775:794	We define ATP6AP1 as a structural hub for Vo complex assembly because it connects to multiple Vo subunits and phospholipids in the c-ring.					
33065002	7	46	theme	integrated	1051:1060	arg1	roles					1062:1066	the integrated roles	1047:1066	the integrated roles of ATP6AP1, glycans, and lipids	1047:1098	This study identifies mechanisms of V-ATPase assembly and biogenesis that rely on the integrated roles of ATP6AP1, glycans, and lipids.					
33065002	5	47	theme	structural	713:722	arg1	hub					724:726	a structural hub	711:726	a structural hub for Vo complex assembly	711:750	We define ATP6AP1 as a structural hub for Vo complex assembly because it connects to multiple Vo subunits and phospholipids in the c-ring.					
33065002	5	47	theme	structural	713:722	arg1	ATP6AP1					700:706	ATP6AP1	700:706	ATP6AP1	700:706	We define ATP6AP1 as a structural hub for Vo complex assembly because it connects to multiple Vo subunits and phospholipids in the c-ring.					
33065002	4	48	theme	mass	540:543	arg1	spectrometry					545:556	mass spectrometry	540:556	mass spectrometry	540:556	Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the Vo complex.					
33065002	2	49	from	eukaryotes	396:405	arg1	acidification					311:323	acidification	311:323	acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes	311:405	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	2	50	theme	vesicles	342:349	arg1	acidification					311:323	acidification	311:323	acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes	311:405	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	6	51	theme	V-ATPase	918:925	arg1	folding					927:933	V-ATPase folding	918:933	V-ATPase folding	918:933	The glycolipids and the glycosylated Vo subunits form a luminal glycan coat critical for V-ATPase folding, localization, and stability.					
33065002	7	52	theme	biogenesis	1023:1032	arg1	mechanisms					987:996	mechanisms	987:996	mechanisms of V-ATPase assembly and biogenesis that rely on the integrated roles of ATP6AP1, glycans, and lipids	987:1098	This study identifies mechanisms of V-ATPase assembly and biogenesis that rely on the integrated roles of ATP6AP1, glycans, and lipids.					
33065002	4	53	with	subunits	586:593	arg1	glycans					620:626	associated N-linked glycans	600:626	associated N-linked glycans	600:626	Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the Vo complex.					
33065002	7	54	theme	V-ATPase	1001:1008	arg1	assembly					1010:1017	V-ATPase assembly	1001:1017	V-ATPase assembly	1001:1017	This study identifies mechanisms of V-ATPase assembly and biogenesis that rely on the integrated roles of ATP6AP1, glycans, and lipids.					
33065002	2	55	theme	intracellular	328:340	arg1	vesicles					342:349	intracellular vesicles	328:349	intracellular vesicles	328:349	They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes.					
33065002	3	56	dep	2.9-Å	513:517	arg1	to					510:511	to	510:511	to	510:511	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	4	57	theme	associated	600:609	arg1	glycans					620:626	associated N-linked glycans	600:626	associated N-linked glycans	600:626	Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the Vo complex.					
33065002	7	58	theme	assembly	1010:1017	arg1	mechanisms					987:996	mechanisms	987:996	mechanisms of V-ATPase assembly and biogenesis that rely on the integrated roles of ATP6AP1, glycans, and lipids	987:1098	This study identifies mechanisms of V-ATPase assembly and biogenesis that rely on the integrated roles of ATP6AP1, glycans, and lipids.					
33065002	3	59	theme	rotational	486:495	arg1	states					497:502	three rotational states	480:502	three rotational states at up to 2.9-Å resolution	480:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	6	60	theme	glycosylated	853:864	arg1	subunits					869:876	the glycosylated Vo subunits	849:876	the glycosylated Vo subunits	849:876	The glycolipids and the glycosylated Vo subunits form a luminal glycan coat critical for V-ATPase folding, localization, and stability.					
33065002	3	61	theme	human	462:466	arg1	V-ATPase					468:475	human V-ATPase	462:475	human V-ATPase in three rotational states at up to 2.9-Å resolution	462:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	3	62	theme	V-ATPase	468:475	arg1	structures					448:457	cryoelectron microscopy structures	424:457	cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution	424:528	Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution.					
33065002	4	63	theme	N-linked	611:618	arg1	glycans					620:626	associated N-linked glycans	600:626	associated N-linked glycans	600:626	Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the Vo complex.					
33065002	1	64	theme	ATP-driven	145:154	arg1	triphosphatases					113:127	vacuolar-type adenosine triphosphatases	89:127	vacuolar-type adenosine triphosphatases (V-ATPases)	89:139	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	64	theme	ATP-driven	145:154	arg1	Vesicular-					75:84	Vesicular-	75:84	Vesicular-	75:84	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	64	theme	ATP-driven	145:154	arg1	pumps					163:167	ATP-driven proton pumps	145:167	ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer	145:279	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
33065002	1	65	theme	membrane-embedded	232:248	arg1	complex					253:259	a membrane-embedded Vo complex	230:259	a membrane-embedded Vo complex for proton transfer	230:279	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V1 complex for ATP hydrolysis and a membrane-embedded Vo complex for proton transfer.					
34218176	4	0	theme	water-induced	968:980	arg1	abilities					995:1003	easy water-induced self-healing abilities	963:1003	easy water-induced self-healing abilities	963:1003	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	7	1	from	range	1452:1456	arg1	regions					1424:1430	two separate regions	1411:1430	two separate regions at different strain range due to its percolated network	1411:1486	It was also discovered that the sensitivity of this type of conductive-filler electronic skin can be divided into two separate regions at different strain range due to its percolated network.					
34218176	7	2	theme	type	1349:1352	arg1	sensitivity					1329:1339	the sensitivity	1325:1339	the sensitivity of this type of conductive-filler electronic skin	1325:1389	It was also discovered that the sensitivity of this type of conductive-filler electronic skin can be divided into two separate regions at different strain range due to its percolated network.					
34218176	9	3	theme	commercial	1784:1793	arg1	sensors					1805:1811	commercial synthetic sensors	1784:1811	commercial synthetic sensors	1784:1811	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	3	4	theme	composite	629:637	arg1	film					639:642	a versatile nature skin-inspired composite film	596:642	a versatile nature skin-inspired composite film as flexible strain biosensor	596:671	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	1	5	theme	health	304:309	arg1	monitoring					311:320	health monitoring	304:320	health monitoring	304:320	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	3	6	theme	nature	608:613	arg1	film					639:642	a versatile nature skin-inspired composite film	596:642	a versatile nature skin-inspired composite film as flexible strain biosensor	596:671	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	6	7	theme	overall	1271:1277	arg1	property					1287:1294	the best overall sensing property	1262:1294	the best overall sensing property	1262:1294	The percolation threshold of CNC-polyaniline (PANI) was determined at 4.278% and 5% CNC-PANI composite film shows the best overall sensing property.					
34218176	3	8	theme	strain	656:661	arg1	biosensor					663:671	flexible strain biosensor	647:671	flexible strain biosensor	647:671	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	1	9	theme	human	223:227	arg1	signals					243:249	human physiological signals	223:249	human physiological signals	223:249	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	1	10	theme	signals	243:249	arg1	method					271:276	an effective method	258:276	an effective method for disease diagnosis and health monitoring	258:320	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	1	10	theme	signals	243:249	arg1	detection					210:218	The conventional skin sensor detection	181:218	The conventional skin sensor detection of human physiological signals	181:249	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	0	11	theme	sensing	162:168	arg1	mechanism					170:178	sensing mechanism	162:178	sensing mechanism	162:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	11	theme	sensing	162:168	arg1	nanocomposites					102:115	robust transparent conductive nanocellulose nanocomposites	58:115	robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism	58:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	6	12	theme	percolation	1152:1162	arg1	threshold					1164:1172	The percolation threshold	1148:1172	The percolation threshold of CNC-polyaniline (PANI)	1148:1198	The percolation threshold of CNC-polyaniline (PANI) was determined at 4.278% and 5% CNC-PANI composite film shows the best overall sensing property.					
34218176	0	13	theme	transparent	65:75	arg1	Relationship					118:129	Relationship	118:129	Relationship between percolated network	118:156	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	13	theme	transparent	65:75	arg1	nanocomposites					102:115	robust transparent conductive nanocellulose nanocomposites	58:115	robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism	58:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	13	theme	transparent	65:75	arg1	mechanism					170:178	sensing mechanism	162:178	sensing mechanism	162:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	3	14	theme	cellulose	696:704	arg1	CNC-PANI					732:739	CNC-PANI	732:739	CNC-PANI	732:739	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	3	14	theme	cellulose	696:704	arg1	nanocrystals-polyaniline					706:729	cellulose nanocrystals-polyaniline	696:729	cellulose nanocrystals-polyaniline (CNC-PANI) composites	696:751	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	8	15	theme	unique	1592:1597	arg1	change					1599:1604	this unique change	1587:1604	this unique change in electrical property	1587:1627	With films prepared by dry casting and dip coating, the alignment of CNC-PANI also contributes to this unique change in electrical property.					
34218176	9	16	dep	mechanism	1670:1678	arg1	the					1666:1668	the	1666:1668	the	1666:1668	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	0	17	theme	nanocellulose	88:100	arg1	Relationship					118:129	Relationship	118:129	Relationship between percolated network	118:156	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	17	theme	nanocellulose	88:100	arg1	nanocomposites					102:115	robust transparent conductive nanocellulose nanocomposites	58:115	robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism	58:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	17	theme	nanocellulose	88:100	arg1	mechanism					170:178	sensing mechanism	162:178	sensing mechanism	162:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	1	18	theme	complex	374:380	arg1	design					382:387	complex design	374:387	complex design	374:387	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	1	19	theme	skin	198:201	arg1	method					271:276	an effective method	258:276	an effective method for disease diagnosis and health monitoring	258:320	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	1	19	theme	skin	198:201	arg1	detection					210:218	The conventional skin sensor detection	181:218	The conventional skin sensor detection of human physiological signals	181:249	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	5	20	theme	sensory	1088:1094	arg1	behavior					1096:1103	the sensory behavior	1084:1103	the sensory behavior determined by CNC nanocomposite alignment	1084:1145	Moreover, we investigated the functioning mechanism of percolated network and the sensory behavior determined by CNC nanocomposite alignment.					
34218176	4	21	theme	mechanical	881:890	arg1	50.62 MPa					902:910	50.62 MPa	902:910	50.62 MPa	902:910	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	4	21	theme	mechanical	881:890	arg1	strength					892:899	robust mechanical strength	874:899	robust mechanical strength (50.62 MPa)	874:911	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	2	22	theme	nanofiller-reinforced	442:462	arg1	composites					464:473	natural nanofiller-reinforced composites	434:473	natural nanofiller-reinforced composites	434:473	Developing natural nanofiller-reinforced composites as strain biosensors is an appealing solution to reduce environmental impacts and overcome technical bottleneck.					
34218176	2	22	theme	nanofiller-reinforced	442:462	arg1	biosensors					485:494	strain biosensors	478:494	strain biosensors	478:494	Developing natural nanofiller-reinforced composites as strain biosensors is an appealing solution to reduce environmental impacts and overcome technical bottleneck.					
34218176	5	23	theme	percolated	1061:1070	arg1	network					1072:1078	percolated network	1061:1078	percolated network	1061:1078	Moreover, we investigated the functioning mechanism of percolated network and the sensory behavior determined by CNC nanocomposite alignment.					
34218176	7	24	theme	different	1435:1443	arg1	range					1452:1456	different strain range	1435:1456	different strain range due to its percolated network	1435:1486	It was also discovered that the sensitivity of this type of conductive-filler electronic skin can be divided into two separate regions at different strain range due to its percolated network.					
34218176	4	25	theme	electronic	851:860	arg1	skin					862:865	The composite electronic skin	837:865	The composite electronic skin	837:865	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	4	26	theme	high	917:920	arg1	sensitivity					922:932	high sensitivity	917:932	high sensitivity (Gauge Factor = 11.467)	917:956	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	4	26	theme	high	917:920	arg1	Factor = 11.467					941:955	Gauge Factor = 11.467	935:955	Gauge Factor = 11.467	935:955	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	0	27	theme	self-healable	17:29	arg1	strain					31:36	Highly sensitive self-healable strain	0:36	Highly sensitive self-healable strain	0:36	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	6	28	dep	%	1223:1223	arg1	shows					1256:1260	shows	1256:1260	shows the best overall sensing property	1256:1294	The percolation threshold of CNC-polyaniline (PANI) was determined at 4.278% and 5% CNC-PANI composite film shows the best overall sensing property.					
34218176	6	28	dep	%	1223:1223	arg1	film					1251:1254	CNC-PANI composite film	1232:1254	CNC-PANI composite film	1232:1254	The percolation threshold of CNC-polyaniline (PANI) was determined at 4.278% and 5% CNC-PANI composite film shows the best overall sensing property.					
34218176	1	29	theme	poor	331:334	arg1	biocompatibility					336:351	the poor biocompatibility	327:351	the poor biocompatibility	327:351	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	9	30	theme	nanofiller-based	1709:1724	arg1	biosensors					1743:1752	conductive nanofiller-based composite strain biosensors	1698:1752	conductive nanofiller-based composite strain biosensors	1698:1752	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	9	31	theme	strain	1736:1741	arg1	biosensors					1743:1752	conductive nanofiller-based composite strain biosensors	1698:1752	conductive nanofiller-based composite strain biosensors	1698:1752	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	9	32	theme	synthetic	1795:1803	arg1	sensors					1805:1811	commercial synthetic sensors	1784:1811	commercial synthetic sensors	1784:1811	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	6	33	theme	composite	1241:1249	arg1	film					1251:1254	CNC-PANI composite film	1232:1254	CNC-PANI composite film	1232:1254	The percolation threshold of CNC-polyaniline (PANI) was determined at 4.278% and 5% CNC-PANI composite film shows the best overall sensing property.					
34218176	3	34	theme	conductive	783:792	arg1	network					794:800	their percolated conductive network	766:800	their percolated conductive network in polyvinyl alcohol (PVA) matrix	766:834	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	8	35	theme	dip	1528:1530	arg1	coating					1532:1538	dip coating	1528:1538	dip coating	1528:1538	With films prepared by dry casting and dip coating, the alignment of CNC-PANI also contributes to this unique change in electrical property.					
34218176	5	36	theme	CNC	1119:1121	arg1	alignment					1137:1145	CNC nanocomposite alignment	1119:1145	CNC nanocomposite alignment	1119:1145	Moreover, we investigated the functioning mechanism of percolated network and the sensory behavior determined by CNC nanocomposite alignment.					
34218176	9	37	theme	potential	1759:1767	arg1	alternative					1769:1779	a potential alternative	1757:1779	a potential alternative to commercial synthetic sensors	1757:1811	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	9	37	theme	potential	1759:1767	arg1	tunability					1684:1693	tunability	1684:1693	tunability	1684:1693	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	9	37	theme	potential	1759:1767	arg1	mechanism					1670:1678	mechanism	1670:1678	mechanism	1670:1678	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	7	38	theme	electronic	1375:1384	arg1	skin					1386:1389	conductive-filler electronic skin	1357:1389	conductive-filler electronic skin	1357:1389	It was also discovered that the sensitivity of this type of conductive-filler electronic skin can be divided into two separate regions at different strain range due to its percolated network.					
34218176	3	39	theme	alcohol	815:821	arg1	matrix					829:834	polyvinyl alcohol (PVA) matrix	805:834	polyvinyl alcohol (PVA) matrix	805:834	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	1	40	theme	disease	282:288	arg1	diagnosis					290:298	disease diagnosis	282:298	disease diagnosis	282:298	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	4	41	theme	easy	963:966	arg1	abilities					995:1003	easy water-induced self-healing abilities	963:1003	easy water-induced self-healing abilities	963:1003	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	3	42	theme	skin-inspired	615:627	arg1	film					639:642	a versatile nature skin-inspired composite film	596:642	a versatile nature skin-inspired composite film as flexible strain biosensor	596:671	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	4	43	theme	self-healing	982:993	arg1	abilities					995:1003	easy water-induced self-healing abilities	963:1003	easy water-induced self-healing abilities	963:1003	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	3	44	theme	versatile	598:606	arg1	film					639:642	a versatile nature skin-inspired composite film	596:642	a versatile nature skin-inspired composite film as flexible strain biosensor	596:671	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	1	45	theme	sensor	203:208	arg1	method					271:276	an effective method	258:276	an effective method for disease diagnosis and health monitoring	258:320	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	1	45	theme	sensor	203:208	arg1	detection					210:218	The conventional skin sensor detection	181:218	The conventional skin sensor detection of human physiological signals	181:249	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	4	46	theme	Gauge	935:939	arg1	sensitivity					922:932	high sensitivity	917:932	high sensitivity (Gauge Factor = 11.467)	917:956	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	4	46	theme	Gauge	935:939	arg1	Factor = 11.467					941:955	Gauge Factor = 11.467	935:955	Gauge Factor = 11.467	935:955	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	6	47	theme	sensing	1279:1285	arg1	property					1287:1294	the best overall sensing property	1262:1294	the best overall sensing property	1262:1294	The percolation threshold of CNC-polyaniline (PANI) was determined at 4.278% and 5% CNC-PANI composite film shows the best overall sensing property.					
34218176	3	48	theme	flexible	647:654	arg1	biosensor					663:671	flexible strain biosensor	647:671	flexible strain biosensor	647:671	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	0	49	dep	biosensors	38:47	arg1	based					49:53	based	49:53	biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism	38:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	1	50	theme	physiological	229:241	arg1	signals					243:249	human physiological signals	223:249	human physiological signals	223:249	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	3	51	from	network	794:800	arg1	matrix					829:834	polyvinyl alcohol (PVA) matrix	805:834	polyvinyl alcohol (PVA) matrix	805:834	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	8	52	theme	CNC-PANI	1558:1565	arg1	alignment					1545:1553	the alignment	1541:1553	the alignment of CNC-PANI	1541:1565	With films prepared by dry casting and dip coating, the alignment of CNC-PANI also contributes to this unique change in electrical property.					
34218176	3	53	theme	nanocrystals-polyaniline	706:729	arg1	composites					742:751	cellulose nanocrystals-polyaniline (CNC-PANI) composites	696:751	cellulose nanocrystals-polyaniline (CNC-PANI) composites	696:751	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	5	54	theme	functioning	1036:1046	arg1	mechanism					1048:1056	the functioning mechanism	1032:1056	the functioning mechanism of percolated network and the sensory behavior determined by CNC nanocomposite alignment	1032:1145	Moreover, we investigated the functioning mechanism of percolated network and the sensory behavior determined by CNC nanocomposite alignment.					
34218176	7	55	theme	percolated	1469:1478	arg1	network					1480:1486	its percolated network	1465:1486	its percolated network	1465:1486	It was also discovered that the sensitivity of this type of conductive-filler electronic skin can be divided into two separate regions at different strain range due to its percolated network.					
34218176	7	56	theme	skin	1386:1389	arg1	type					1349:1352	this type	1344:1352	this type of conductive-filler electronic skin	1344:1389	It was also discovered that the sensitivity of this type of conductive-filler electronic skin can be divided into two separate regions at different strain range due to its percolated network.					
34218176	0	57	theme	conductive	77:86	arg1	Relationship					118:129	Relationship	118:129	Relationship between percolated network	118:156	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	57	theme	conductive	77:86	arg1	nanocomposites					102:115	robust transparent conductive nanocellulose nanocomposites	58:115	robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism	58:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	57	theme	conductive	77:86	arg1	mechanism					170:178	sensing mechanism	162:178	sensing mechanism	162:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	2	58	theme	technical	566:574	arg1	bottleneck					576:585	technical bottleneck	566:585	technical bottleneck	566:585	Developing natural nanofiller-reinforced composites as strain biosensors is an appealing solution to reduce environmental impacts and overcome technical bottleneck.					
34218176	1	59	theme	conventional	185:196	arg1	method					271:276	an effective method	258:276	an effective method for disease diagnosis and health monitoring	258:320	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	1	59	theme	conventional	185:196	arg1	detection					210:218	The conventional skin sensor detection	181:218	The conventional skin sensor detection of human physiological signals	181:249	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	8	60	theme	electrical	1609:1618	arg1	property					1620:1627	electrical property	1609:1627	electrical property	1609:1627	With films prepared by dry casting and dip coating, the alignment of CNC-PANI also contributes to this unique change in electrical property.					
34218176	0	61	theme	percolated	139:148	arg1	network					150:156	percolated network	139:156	percolated network	139:156	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	5	62	theme	behavior	1096:1103	arg1	mechanism					1048:1056	the functioning mechanism	1032:1056	the functioning mechanism of percolated network and the sensory behavior determined by CNC nanocomposite alignment	1032:1145	Moreover, we investigated the functioning mechanism of percolated network and the sensory behavior determined by CNC nanocomposite alignment.					
34218176	7	63	theme	strain	1445:1450	arg1	range					1452:1456	different strain range	1435:1456	different strain range due to its percolated network	1435:1486	It was also discovered that the sensitivity of this type of conductive-filler electronic skin can be divided into two separate regions at different strain range due to its percolated network.					
34218176	4	64	theme	robust	874:879	arg1	50.62 MPa					902:910	50.62 MPa	902:910	50.62 MPa	902:910	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	4	64	theme	robust	874:879	arg1	strength					892:899	robust mechanical strength	874:899	robust mechanical strength (50.62 MPa)	874:911	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	2	65	theme	natural	434:440	arg1	composites					464:473	natural nanofiller-reinforced composites	434:473	natural nanofiller-reinforced composites	434:473	Developing natural nanofiller-reinforced composites as strain biosensors is an appealing solution to reduce environmental impacts and overcome technical bottleneck.					
34218176	2	65	theme	natural	434:440	arg1	biosensors					485:494	strain biosensors	478:494	strain biosensors	478:494	Developing natural nanofiller-reinforced composites as strain biosensors is an appealing solution to reduce environmental impacts and overcome technical bottleneck.					
34218176	0	66	theme	sensitive	7:15	arg1	strain					31:36	Highly sensitive self-healable strain	0:36	Highly sensitive self-healable strain	0:36	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	67	dep	nanocomposites	102:115	arg1	Relationship					118:129	Relationship	118:129	Relationship between percolated network	118:156	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	67	dep	nanocomposites	102:115	arg1	mechanism					170:178	sensing mechanism	162:178	sensing mechanism	162:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	67	dep	nanocomposites	102:115	arg1	nanocomposites					102:115	robust transparent conductive nanocellulose nanocomposites	58:115	robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism	58:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	2	68	theme	appealing	502:510	arg1	solution					512:519	an appealing solution	499:519	an appealing solution to reduce environmental impacts and overcome technical bottleneck	499:585	Developing natural nanofiller-reinforced composites as strain biosensors is an appealing solution to reduce environmental impacts and overcome technical bottleneck.					
34218176	8	69	from	change	1599:1604	arg1	property					1620:1627	electrical property	1609:1627	electrical property	1609:1627	With films prepared by dry casting and dip coating, the alignment of CNC-PANI also contributes to this unique change in electrical property.					
34218176	5	70	theme	network	1072:1078	arg1	mechanism					1048:1056	the functioning mechanism	1032:1056	the functioning mechanism of percolated network and the sensory behavior determined by CNC nanocomposite alignment	1032:1145	Moreover, we investigated the functioning mechanism of percolated network and the sensory behavior determined by CNC nanocomposite alignment.					
34218176	7	71	theme	separate	1415:1422	arg1	regions					1424:1430	two separate regions	1411:1430	two separate regions at different strain range due to its percolated network	1411:1486	It was also discovered that the sensitivity of this type of conductive-filler electronic skin can be divided into two separate regions at different strain range due to its percolated network.					
34218176	4	72	theme	composite	841:849	arg1	skin					862:865	The composite electronic skin	837:865	The composite electronic skin	837:865	The composite electronic skin showed robust mechanical strength (50.62 MPa) and high sensitivity (Gauge Factor = 11.467) with easy water-induced self-healing abilities.					
34218176	2	73	theme	strain	478:483	arg1	composites					464:473	natural nanofiller-reinforced composites	434:473	natural nanofiller-reinforced composites	434:473	Developing natural nanofiller-reinforced composites as strain biosensors is an appealing solution to reduce environmental impacts and overcome technical bottleneck.					
34218176	2	73	theme	strain	478:483	arg1	biosensors					485:494	strain biosensors	478:494	strain biosensors	478:494	Developing natural nanofiller-reinforced composites as strain biosensors is an appealing solution to reduce environmental impacts and overcome technical bottleneck.					
34218176	9	74	theme	conductive	1698:1707	arg1	biosensors					1743:1752	conductive nanofiller-based composite strain biosensors	1698:1752	conductive nanofiller-based composite strain biosensors	1698:1752	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	6	75	theme	CNC-polyaniline	1177:1191	arg1	threshold					1164:1172	The percolation threshold	1148:1172	The percolation threshold of CNC-polyaniline (PANI)	1148:1198	The percolation threshold of CNC-polyaniline (PANI) was determined at 4.278% and 5% CNC-PANI composite film shows the best overall sensing property.					
34218176	2	76	theme	environmental	531:543	arg1	impacts					545:551	environmental impacts	531:551	environmental impacts	531:551	Developing natural nanofiller-reinforced composites as strain biosensors is an appealing solution to reduce environmental impacts and overcome technical bottleneck.					
34218176	0	77	theme	robust	58:63	arg1	Relationship					118:129	Relationship	118:129	Relationship between percolated network	118:156	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	77	theme	robust	58:63	arg1	nanocomposites					102:115	robust transparent conductive nanocellulose nanocomposites	58:115	robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism	58:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	0	77	theme	robust	58:63	arg1	mechanism					170:178	sensing mechanism	162:178	sensing mechanism	162:178	Highly sensitive self-healable strain biosensors based on robust transparent conductive nanocellulose nanocomposites: Relationship between percolated network and sensing mechanism.					
34218176	9	78	theme	composite	1726:1734	arg1	biosensors					1743:1752	conductive nanofiller-based composite strain biosensors	1698:1752	conductive nanofiller-based composite strain biosensors	1698:1752	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	3	79	theme	percolated	772:781	arg1	network					794:800	their percolated conductive network	766:800	their percolated conductive network in polyvinyl alcohol (PVA) matrix	766:834	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	8	80	theme	dry	1512:1514	arg1	casting					1516:1522	dry casting	1512:1522	dry casting	1512:1522	With films prepared by dry casting and dip coating, the alignment of CNC-PANI also contributes to this unique change in electrical property.					
34218176	1	81	theme	low	354:356	arg1	sensitivity					358:368	low sensitivity	354:368	low sensitivity	354:368	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	9	82	theme	biosensors	1743:1752	arg1	alternative					1769:1779	a potential alternative	1757:1779	a potential alternative to commercial synthetic sensors	1757:1811	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	9	82	theme	biosensors	1743:1752	arg1	tunability					1684:1693	tunability	1684:1693	tunability	1684:1693	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	9	82	theme	biosensors	1743:1752	arg1	mechanism					1670:1678	mechanism	1670:1678	mechanism	1670:1678	Generally, our results demonstrated the mechanism and tunability of conductive nanofiller-based composite strain biosensors as a potential alternative to commercial synthetic sensors.					
34218176	6	83	theme	CNC-PANI	1232:1239	arg1	film					1251:1254	CNC-PANI composite film	1232:1254	CNC-PANI composite film	1232:1254	The percolation threshold of CNC-polyaniline (PANI) was determined at 4.278% and 5% CNC-PANI composite film shows the best overall sensing property.					
34218176	1	84	theme	effective	261:269	arg1	method					271:276	an effective method	258:276	an effective method for disease diagnosis and health monitoring	258:320	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	1	84	theme	effective	261:269	arg1	detection					210:218	The conventional skin sensor detection	181:218	The conventional skin sensor detection of human physiological signals	181:249	The conventional skin sensor detection of human physiological signals can be an effective method for disease diagnosis and health monitoring, but the poor biocompatibility, low sensitivity and complex design largely limit their applications.					
34218176	5	85	theme	nanocomposite	1123:1135	arg1	alignment					1137:1145	CNC nanocomposite alignment	1119:1145	CNC nanocomposite alignment	1119:1145	Moreover, we investigated the functioning mechanism of percolated network and the sensory behavior determined by CNC nanocomposite alignment.					
34218176	7	86	theme	conductive-filler	1357:1373	arg1	skin					1386:1389	conductive-filler electronic skin	1357:1389	conductive-filler electronic skin	1357:1389	It was also discovered that the sensitivity of this type of conductive-filler electronic skin can be divided into two separate regions at different strain range due to its percolated network.					
34218176	3	87	theme	polyvinyl	805:813	arg1	PVA					824:826	PVA	824:826	PVA	824:826	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
34218176	3	87	theme	polyvinyl	805:813	arg1	alcohol					815:821	polyvinyl alcohol	805:821	polyvinyl alcohol (PVA) matrix	805:834	Herein, a versatile nature skin-inspired composite film as flexible strain biosensor was developed based on cellulose nanocrystals-polyaniline (CNC-PANI) composites by utilizing their percolated conductive network in polyvinyl alcohol (PVA) matrix.					
33750134	5	0	theme	mice	872:875	arg1	microbiota					858:867	the gut microbiota	850:867	the gut microbiota of mice	850:875	The effects of dietary riclinoctaose on the gut microbiota of mice were evaluated.					
33750134	1	1	contain	have	148:151	arg2	weight					169:174	a low molecular weight	153:174	a low molecular weight	153:174	In relation to available polysaccharides, oligosaccharides have a low molecular weight, less viscosity, and complete water solubility.					
33750134	1	1	contain	have	148:151	arg2	solubility					212:221	complete water solubility	197:221	complete water solubility	197:221	In relation to available polysaccharides, oligosaccharides have a low molecular weight, less viscosity, and complete water solubility.					
33750134	1	1	contain	have	148:151	arg1	oligosaccharides					131:146	oligosaccharides	131:146	oligosaccharides	131:146	In relation to available polysaccharides, oligosaccharides have a low molecular weight, less viscosity, and complete water solubility.					
33750134	1	1	contain	have	148:151	arg2	viscosity					182:190	less viscosity	177:190	less viscosity	177:190	In relation to available polysaccharides, oligosaccharides have a low molecular weight, less viscosity, and complete water solubility.					
33750134	6	2	theme	bacteria	1028:1035	arg1	growth					996:1001	the increased growth	982:1001	the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus	982:1078	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	4	3	with	oligooctasaccharide	672:690	arg1	group					764:768	a pyruvate group	753:768	a pyruvate group linked to the terminal glucose residue	753:807	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	5	4	theme	riclinoctaose	833:845	arg1	effects					814:820	The effects	810:820	The effects of dietary riclinoctaose on the gut microbiota of mice	810:875	The effects of dietary riclinoctaose on the gut microbiota of mice were evaluated.					
33750134	4	5	theme	magnetic	596:603	arg1	resonance					605:613	nuclear magnetic resonance	588:613	nuclear magnetic resonance	588:613	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	6	6	theme	increased	986:994	arg1	growth					996:1001	the increased growth	982:1001	the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus	982:1078	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	2	7	theme	regulation	328:337	arg1	ability					339:345	the microbiota regulation ability	313:345	the microbiota regulation ability	313:345	These properties endow oligosaccharides with significant biological properties including the microbiota regulation ability.					
33750134	4	8	theme	mass	565:568	arg1	spectrometry					570:581	Fourier-transform infrared, electrospray ionization mass spectrometry	513:581	spectrometry	570:581	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	2	9	theme	microbiota	317:326	arg1	ability					339:345	the microbiota regulation ability	313:345	the microbiota regulation ability	313:345	These properties endow oligosaccharides with significant biological properties including the microbiota regulation ability.					
33750134	5	10	from	effects	814:820	arg1	microbiota					858:867	the gut microbiota	850:867	the gut microbiota of mice	850:875	The effects of dietary riclinoctaose on the gut microbiota of mice were evaluated.					
33750134	8	11	theme	great	1327:1331	arg1	application					1343:1353	a great potential application	1325:1353	a great potential application	1325:1353	Our results suggested that riclinoctaose as a prebiotic may have a great potential application in functional foods.					
33750134	4	12	theme	ionization	554:563	arg1	spectrometry					570:581	Fourier-transform infrared, electrospray ionization mass spectrometry	513:581	spectrometry	570:581	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	5	13	theme	gut	854:856	arg1	microbiota					858:867	the gut microbiota	850:867	the gut microbiota of mice	850:875	The effects of dietary riclinoctaose on the gut microbiota of mice were evaluated.					
33750134	6	14	theme	beneficial	1006:1015	arg1	Bifidobacteria					1047:1060	Bifidobacteria	1047:1060	Bifidobacteria	1047:1060	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	6	14	theme	beneficial	1006:1015	arg1	bacteria					1028:1035	beneficial intestinal bacteria	1006:1035	beneficial intestinal bacteria including Bifidobacteria and Lactobacillus	1006:1078	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	6	14	theme	beneficial	1006:1015	arg1	Lactobacillus					1066:1078	Lactobacillus	1066:1078	Lactobacillus	1066:1078	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	8	15	contain	have	1320:1323	arg1	riclinoctaose					1287:1299	riclinoctaose	1287:1299	riclinoctaose as a prebiotic	1287:1314	Our results suggested that riclinoctaose as a prebiotic may have a great potential application in functional foods.					
33750134	8	15	contain	have	1320:1323	arg2	application					1343:1353	a great potential application	1325:1353	a great potential application	1325:1353	Our results suggested that riclinoctaose as a prebiotic may have a great potential application in functional foods.					
33750134	8	16	theme	potential	1333:1341	arg1	application					1343:1353	a great potential application	1325:1353	a great potential application	1325:1353	Our results suggested that riclinoctaose as a prebiotic may have a great potential application in functional foods.					
33750134	6	17	theme	intestinal	1017:1026	arg1	Bifidobacteria					1047:1060	Bifidobacteria	1047:1060	Bifidobacteria	1047:1060	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	6	17	theme	intestinal	1017:1026	arg1	bacteria					1028:1035	beneficial intestinal bacteria	1006:1035	beneficial intestinal bacteria including Bifidobacteria and Lactobacillus	1006:1078	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	6	17	theme	intestinal	1017:1026	arg1	Lactobacillus					1066:1078	Lactobacillus	1066:1078	Lactobacillus	1066:1078	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	6	18	theme	pernicious	1111:1120	arg1	bacteria					1122:1129	pernicious bacteria	1111:1129	pernicious bacteria such as Gammaproteobacteria	1111:1157	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	6	18	theme	pernicious	1111:1120	arg1	Gammaproteobacteria					1139:1157	Gammaproteobacteria	1139:1157	Gammaproteobacteria	1139:1157	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	3	19	theme	succinylglycan	437:450	arg1	riclin					452:457	succinylglycan riclin	437:457	succinylglycan riclin	437:457	In this study, a homogeneous oligooctasaccharide, riclinoctaose, was biosynthesized from succinylglycan riclin by enzymatic degradation.					
33750134	7	20	theme	riclinoctaose	1239:1251	arg1	cecum					1253:1257	the riclinoctaose cecum	1235:1257	the riclinoctaose cecum	1235:1257	The level of short-chain fatty acids (SCFAs) was significantly elevated in the riclinoctaose cecum.					
33750134	4	21	theme	Fourier-transform	513:529	arg1	spectrometry					570:581	Fourier-transform infrared, electrospray ionization mass spectrometry	513:581	spectrometry	570:581	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	8	22	theme	functional	1358:1367	arg1	foods					1369:1373	functional foods	1358:1373	functional foods	1358:1373	Our results suggested that riclinoctaose as a prebiotic may have a great potential application in functional foods.					
33750134	6	23	theme	bacteria	1122:1129	arg1	abundance					1098:1106	the abundance	1094:1106	the abundance of pernicious bacteria such as Gammaproteobacteria	1094:1157	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	1	24	theme	available	104:112	arg1	polysaccharides					114:128	available polysaccharides	104:128	available polysaccharides	104:128	In relation to available polysaccharides, oligosaccharides have a low molecular weight, less viscosity, and complete water solubility.					
33750134	1	25	theme	complete	197:204	arg1	solubility					212:221	complete water solubility	197:221	complete water solubility	197:221	In relation to available polysaccharides, oligosaccharides have a low molecular weight, less viscosity, and complete water solubility.					
33750134	2	26	with	oligosaccharides	247:262	arg1	ability					339:345	the microbiota regulation ability	313:345	the microbiota regulation ability	313:345	These properties endow oligosaccharides with significant biological properties including the microbiota regulation ability.					
33750134	2	26	with	oligosaccharides	247:262	arg1	properties					292:301	significant biological properties	269:301	significant biological properties including the microbiota regulation ability	269:345	These properties endow oligosaccharides with significant biological properties including the microbiota regulation ability.					
33750134	4	27	theme	infrared	531:538	arg1	spectrometry					570:581	Fourier-transform infrared, electrospray ionization mass spectrometry	513:581	spectrometry	570:581	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	1	28	theme	water	206:210	arg1	solubility					212:221	complete water solubility	197:221	complete water solubility	197:221	In relation to available polysaccharides, oligosaccharides have a low molecular weight, less viscosity, and complete water solubility.					
33750134	3	29	theme	enzymatic	462:470	arg1	degradation					472:482	enzymatic degradation	462:482	enzymatic degradation	462:482	In this study, a homogeneous oligooctasaccharide, riclinoctaose, was biosynthesized from succinylglycan riclin by enzymatic degradation.					
33750134	4	30	attach	linked	770:775	arg1	residue					801:807	the terminal glucose residue	780:807	the terminal glucose residue	780:807	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	4	30	attach	linked	770:775	arg2	group					764:768	a pyruvate group	753:768	a pyruvate group linked to the terminal glucose residue	753:807	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	4	31	theme	pyruvate	755:762	arg1	group					764:768	a pyruvate group	753:768	a pyruvate group linked to the terminal glucose residue	753:807	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	0	32	theme	Modulatory	31:40	arg1	Property					42:49	Modulatory Property	31:49	Modulatory Property of the Oligosaccharide Riclinoctaose	31:86	Preparation and Gut Microbiota Modulatory Property of the Oligosaccharide Riclinoctaose.					
33750134	5	33	theme	dietary	825:831	arg1	riclinoctaose					833:845	dietary riclinoctaose	825:845	dietary riclinoctaose	825:845	The effects of dietary riclinoctaose on the gut microbiota of mice were evaluated.					
33750134	4	34	theme	electrospray	541:552	arg1	spectrometry					570:581	Fourier-transform infrared, electrospray ionization mass spectrometry	513:581	spectrometry	570:581	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	7	35	theme	short-chain	1173:1183	arg1	SCFAs					1198:1202	SCFAs	1198:1202	SCFAs	1198:1202	The level of short-chain fatty acids (SCFAs) was significantly elevated in the riclinoctaose cecum.					
33750134	7	35	theme	short-chain	1173:1183	arg1	acids					1191:1195	short-chain fatty acids	1173:1195	short-chain fatty acids (SCFAs)	1173:1203	The level of short-chain fatty acids (SCFAs) was significantly elevated in the riclinoctaose cecum.					
33750134	4	36	theme	spectrometry	615:626	arg1	analysis					628:635	spectrometry analysis	615:635	spectrometry analysis	615:635	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	3	37	theme	homogeneous	365:375	arg1	riclinoctaose					398:410	riclinoctaose	398:410	riclinoctaose	398:410	In this study, a homogeneous oligooctasaccharide, riclinoctaose, was biosynthesized from succinylglycan riclin by enzymatic degradation.					
33750134	3	37	theme	homogeneous	365:375	arg1	oligooctasaccharide					377:395	a homogeneous oligooctasaccharide	363:395	a homogeneous oligooctasaccharide	363:395	In this study, a homogeneous oligooctasaccharide, riclinoctaose, was biosynthesized from succinylglycan riclin by enzymatic degradation.					
33750134	7	38	theme	fatty	1185:1189	arg1	SCFAs					1198:1202	SCFAs	1198:1202	SCFAs	1198:1202	The level of short-chain fatty acids (SCFAs) was significantly elevated in the riclinoctaose cecum.					
33750134	7	38	theme	fatty	1185:1189	arg1	acids					1191:1195	short-chain fatty acids	1173:1195	short-chain fatty acids (SCFAs)	1173:1203	The level of short-chain fatty acids (SCFAs) was significantly elevated in the riclinoctaose cecum.					
33750134	4	39	theme	Monosaccharide	485:498	arg1	composition					500:510	Monosaccharide composition	485:510	Monosaccharide composition	485:510	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	6	40	theme	intestinal	955:964	arg1	microbiota					966:975	intestinal microbiota	955:975	intestinal microbiota	955:975	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	7	41	theme	acids	1191:1195	arg1	level					1164:1168	The level	1160:1168	The level of short-chain fatty acids (SCFAs)	1160:1203	The level of short-chain fatty acids (SCFAs) was significantly elevated in the riclinoctaose cecum.					
33750134	1	42	theme	low	155:157	arg1	weight					169:174	a low molecular weight	153:174	a low molecular weight	153:174	In relation to available polysaccharides, oligosaccharides have a low molecular weight, less viscosity, and complete water solubility.					
33750134	6	43	theme	dietary	911:917	arg1	riclinoctaose					919:931	the dietary riclinoctaose	907:931	the dietary riclinoctaose	907:931	We found that the dietary riclinoctaose significantly altered intestinal microbiota with the increased growth of beneficial intestinal bacteria including Bifidobacteria and Lactobacillus and decreased the abundance of pernicious bacteria such as Gammaproteobacteria.					
33750134	2	44	theme	biological	281:290	arg1	ability					339:345	the microbiota regulation ability	313:345	the microbiota regulation ability	313:345	These properties endow oligosaccharides with significant biological properties including the microbiota regulation ability.					
33750134	2	44	theme	biological	281:290	arg1	properties					292:301	significant biological properties	269:301	significant biological properties including the microbiota regulation ability	269:345	These properties endow oligosaccharides with significant biological properties including the microbiota regulation ability.					
33750134	4	45	theme	terminal	784:791	arg1	residue					801:807	the terminal glucose residue	780:807	the terminal glucose residue	780:807	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	4	46	dep	composition	500:510	arg1	analysis					628:635	spectrometry analysis	615:635	spectrometry analysis	615:635	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	0	47	theme	Riclinoctaose	74:86	arg1	Property					42:49	Modulatory Property	31:49	Modulatory Property of the Oligosaccharide Riclinoctaose	31:86	Preparation and Gut Microbiota Modulatory Property of the Oligosaccharide Riclinoctaose.					
33750134	2	48	theme	significant	269:279	arg1	ability					339:345	the microbiota regulation ability	313:345	the microbiota regulation ability	313:345	These properties endow oligosaccharides with significant biological properties including the microbiota regulation ability.					
33750134	2	48	theme	significant	269:279	arg1	properties					292:301	significant biological properties	269:301	significant biological properties including the microbiota regulation ability	269:345	These properties endow oligosaccharides with significant biological properties including the microbiota regulation ability.					
33750134	4	49	theme	glucose	793:799	arg1	residue					801:807	the terminal glucose residue	780:807	the terminal glucose residue	780:807	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	0	50	theme	Oligosaccharide	58:72	arg1	Riclinoctaose					74:86	the Oligosaccharide Riclinoctaose	54:86	the Oligosaccharide Riclinoctaose	54:86	Preparation and Gut Microbiota Modulatory Property of the Oligosaccharide Riclinoctaose.					
33750134	4	51	theme	nuclear	588:594	arg1	resonance					605:613	nuclear magnetic resonance	588:613	nuclear magnetic resonance	588:613	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33750134	1	52	theme	molecular	159:167	arg1	weight					169:174	a low molecular weight	153:174	a low molecular weight	153:174	In relation to available polysaccharides, oligosaccharides have a low molecular weight, less viscosity, and complete water solubility.					
33750134	4	53	theme	glucose	730:736	arg1	residues					738:745	seven glucose residues	724:745	seven glucose residues	724:745	Monosaccharide composition, Fourier-transform infrared, electrospray ionization mass spectrometry, and nuclear magnetic resonance spectrometry analysis indicated that riclinoctaose is an oligooctasaccharide consisting of one galactose and seven glucose residues, with a pyruvate group linked to the terminal glucose residue.					
33652043	1	0	theme	dihydromyricetin	169:184	arg1	concentration					152:164	different concentration	142:164	different concentration of dihydromyricetin (DMY)	142:190	Active composite films were developed by incorporating different concentration of dihydromyricetin (DMY) into konjac glucomannan (KGM)/gellan gum (GG) matrix.					
33652043	5	1	theme	DMY	803:805	arg1	incorporation					786:798	the incorporation	782:798	the incorporation of DMY	782:805	In particular, the incorporation of DMY remarkably enhanced the antioxidant and antimicrobial activities of the films.					
33652043	2	2	theme	films	349:353	arg1	properties					325:334	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties	246:334	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films	246:353	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films were investigated.					
33652043	3	3	from	matrix	535:540	arg1	well-dispersed					506:519	well-dispersed	506:519	well-dispersed	506:519	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	4	4	theme	predominant	618:628	arg1	thermostability					630:644	predominant thermostability	618:644	predominant thermostability	618:644	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	6	5	theme	fabricated	899:908	arg1	films					931:935	the fabricated KGM/GG-DMY composite films	895:935	the fabricated KGM/GG-DMY composite films	895:935	Overall, the fabricated KGM/GG-DMY composite films have a promising application in the fields of food packaging.					
33652043	4	6	theme	obtained	593:600	arg1	films					602:606	The obtained films	589:606	The obtained films	589:606	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	3	7	from	well-dispersed	506:519	arg1	matrix					535:540	the KGM/GG matrix	524:540	the KGM/GG matrix	524:540	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	2	8	theme	antimicrobial	311:323	arg1	properties					325:334	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties	246:334	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films	246:353	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films were investigated.					
33652043	4	9	theme	resistance	658:667	arg1	property					669:676	good water resistance property	647:676	good water resistance property	647:676	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	3	10	dep	Fourier	395:401	arg1	transform					403:411	transform	403:411	transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM)	403:479	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	4	11	theme	ultraviolet	689:699	arg1	light					701:705	excellent ultraviolet light	679:705	excellent ultraviolet light barrier ability	679:721	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	5	12	theme	antioxidant	831:841	arg1	activities					861:870	the antioxidant and antimicrobial activities	827:870	the antioxidant and antimicrobial activities of the films	827:883	In particular, the incorporation of DMY remarkably enhanced the antioxidant and antimicrobial activities of the films.					
33652043	3	13	dep	transform	403:411	arg1	infrared					413:420	infrared	413:420	transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM)	403:479	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	3	14	theme	KGM/GG	528:533	arg1	matrix					535:540	the KGM/GG matrix	524:540	the KGM/GG matrix	524:540	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	6	15	theme	composite	921:929	arg1	films					931:935	the fabricated KGM/GG-DMY composite films	895:935	the fabricated KGM/GG-DMY composite films	895:935	Overall, the fabricated KGM/GG-DMY composite films have a promising application in the fields of food packaging.					
33652043	1	16	theme	Active	87:92	arg1	films					104:108	Active composite films	87:108	Active composite films	87:108	Active composite films were developed by incorporating different concentration of dihydromyricetin (DMY) into konjac glucomannan (KGM)/gellan gum (GG) matrix.					
33652043	2	17	dep	Physicochemical	246:260	arg1	released					275:282	released	275:282	released	275:282	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films were investigated.					
33652043	2	17	dep	Physicochemical	246:260	arg1	mechanical					263:272	mechanical	263:272	mechanical	263:272	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films were investigated.					
33652043	5	18	theme	antimicrobial	847:859	arg1	activities					861:870	the antioxidant and antimicrobial activities	827:870	the antioxidant and antimicrobial activities of the films	827:883	In particular, the incorporation of DMY remarkably enhanced the antioxidant and antimicrobial activities of the films.					
33652043	4	19	theme	release	748:754	arg1	behaviour					756:764	sustained controlled release behaviour	727:764	sustained controlled release behaviour	727:764	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	2	20	theme	antioxidant	295:305	arg1	properties					325:334	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties	246:334	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films	246:353	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films were investigated.					
33652043	1	21	theme	konjac	197:202	arg1	matrix					238:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	Active composite films were developed by incorporating different concentration of dihydromyricetin (DMY) into konjac glucomannan (KGM)/gellan gum (GG) matrix.					
33652043	3	22	dep	well-dispersed	506:519	arg1	DMY					496:498	DMY	496:498	DMY which well-dispersed in the KGM/GG matrix	496:540	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	1	23	theme	glucomannan	204:214	arg1	matrix					238:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	Active composite films were developed by incorporating different concentration of dihydromyricetin (DMY) into konjac glucomannan (KGM)/gellan gum (GG) matrix.					
33652043	1	24	theme	composite	94:102	arg1	films					104:108	Active composite films	87:108	Active composite films	87:108	Active composite films were developed by incorporating different concentration of dihydromyricetin (DMY) into konjac glucomannan (KGM)/gellan gum (GG) matrix.					
33652043	6	25	theme	KGM/GG-DMY	910:919	arg1	films					931:935	the fabricated KGM/GG-DMY composite films	895:935	the fabricated KGM/GG-DMY composite films	895:935	Overall, the fabricated KGM/GG-DMY composite films have a promising application in the fields of food packaging.					
33652043	2	26	theme	behaviour	284:292	arg1	properties					325:334	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties	246:334	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films	246:353	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films were investigated.					
33652043	5	27	theme	films	879:883	arg1	activities					861:870	the antioxidant and antimicrobial activities	827:870	the antioxidant and antimicrobial activities of the films	827:883	In particular, the incorporation of DMY remarkably enhanced the antioxidant and antimicrobial activities of the films.					
33652043	1	28	theme	KGM	217:219	arg1	matrix					238:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	Active composite films were developed by incorporating different concentration of dihydromyricetin (DMY) into konjac glucomannan (KGM)/gellan gum (GG) matrix.					
33652043	4	29	theme	excellent	679:687	arg1	light					701:705	excellent ultraviolet light	679:705	excellent ultraviolet light barrier ability	679:721	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	3	30	theme	scanning	446:453	arg1	SEM					476:478	SEM	476:478	SEM	476:478	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	3	30	theme	scanning	446:453	arg1	microscopy					464:473	scanning electron microscopy	446:473	scanning electron microscopy (SEM)	446:479	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	6	31	theme	packaging	988:996	arg1	fields					973:978	the fields	969:978	the fields of food packaging	969:996	Overall, the fabricated KGM/GG-DMY composite films have a promising application in the fields of food packaging.					
33652043	6	32	contain	have	937:940	arg2	application					954:964	a promising application	942:964	a promising application	942:964	Overall, the fabricated KGM/GG-DMY composite films have a promising application in the fields of food packaging.					
33652043	6	32	contain	have	937:940	arg1	films					931:935	the fabricated KGM/GG-DMY composite films	895:935	the fabricated KGM/GG-DMY composite films	895:935	Overall, the fabricated KGM/GG-DMY composite films have a promising application in the fields of food packaging.					
33652043	3	33	theme	electron	455:462	arg1	SEM					476:478	SEM	476:478	SEM	476:478	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	3	33	theme	electron	455:462	arg1	microscopy					464:473	scanning electron microscopy	446:473	scanning electron microscopy (SEM)	446:479	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	6	34	theme	food	983:986	arg1	packaging					988:996	food packaging	983:996	food packaging	983:996	Overall, the fabricated KGM/GG-DMY composite films have a promising application in the fields of food packaging.					
33652043	2	35	theme	composite	339:347	arg1	films					349:353	composite films	339:353	composite films	339:353	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films were investigated.					
33652043	0	36	theme	konjac	52:57	arg1	glucomannan					59:69	konjac glucomannan	52:69	konjac glucomannan	52:69	Dihydromyricetin incorporated active films based on konjac glucomannan and gellan gum.					
33652043	4	37	theme	sustained	727:735	arg1	behaviour					756:764	sustained controlled release behaviour	727:764	sustained controlled release behaviour	727:764	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	4	38	theme	controlled	737:746	arg1	behaviour					756:764	sustained controlled release behaviour	727:764	sustained controlled release behaviour	727:764	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	2	39	theme	Physicochemical	246:260	arg1	properties					325:334	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties	246:334	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films	246:353	Physicochemical, mechanical, released behaviour, antioxidant and antimicrobial properties of composite films were investigated.					
33652043	4	40	theme	light	701:705	arg1	ability					715:721	excellent ultraviolet light barrier ability	679:721	excellent ultraviolet light barrier ability	679:721	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	1	41	theme	/gellan	221:227	arg1	matrix					238:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	Active composite films were developed by incorporating different concentration of dihydromyricetin (DMY) into konjac glucomannan (KGM)/gellan gum (GG) matrix.					
33652043	1	42	theme	different	142:150	arg1	concentration					152:164	different concentration	142:164	different concentration of dihydromyricetin (DMY)	142:190	Active composite films were developed by incorporating different concentration of dihydromyricetin (DMY) into konjac glucomannan (KGM)/gellan gum (GG) matrix.					
33652043	1	43	theme	gum	229:231	arg1	matrix					238:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	Active composite films were developed by incorporating different concentration of dihydromyricetin (DMY) into konjac glucomannan (KGM)/gellan gum (GG) matrix.					
33652043	0	44	theme	gellan	75:80	arg1	gum					82:84	gellan gum	75:84	gellan gum	75:84	Dihydromyricetin incorporated active films based on konjac glucomannan and gellan gum.					
33652043	4	45	theme	water	652:656	arg1	property					669:676	good water resistance property	647:676	good water resistance property	647:676	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	4	46	theme	good	647:650	arg1	property					669:676	good water resistance property	647:676	good water resistance property	647:676	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	4	47	theme	barrier	707:713	arg1	ability					715:721	excellent ultraviolet light barrier ability	679:721	excellent ultraviolet light barrier ability	679:721	The obtained films presented predominant thermostability, good water resistance property, excellent ultraviolet light barrier ability and sustained controlled release behaviour.					
33652043	6	48	theme	promising	944:952	arg1	application					954:964	a promising application	942:964	a promising application	942:964	Overall, the fabricated KGM/GG-DMY composite films have a promising application in the fields of food packaging.					
33652043	3	49	from	Fourier	395:401	arg1	results					378:384	The results	374:384	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM)	374:479	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	0	50	theme	active	30:35	arg1	films					37:41	active films	30:41	active films	30:41	Dihydromyricetin incorporated active films based on konjac glucomannan and gellan gum.					
33652043	3	51	theme	hydrogen	573:580	arg1	bonds					582:586	hydrogen bonds	573:586	hydrogen bonds	573:586	The results from the Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) indicated that DMY which well-dispersed in the KGM/GG matrix interacted with matrix through hydrogen bonds.					
33652043	1	52	theme	GG	234:235	arg1	matrix					238:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	konjac glucomannan (KGM)/gellan gum (GG) matrix	197:243	Active composite films were developed by incorporating different concentration of dihydromyricetin (DMY) into konjac glucomannan (KGM)/gellan gum (GG) matrix.					
34623811	2	0	theme	fecal	492:496	arg1	microbiota					498:507	human fecal microbiota	486:507	human fecal microbiota in an in vitro batch system	486:535	In this study, we prepared starch-entrapped microspheres with a variable chitosan-to-starch ratio by means of electrospraying and investigated the fermentability by human fecal microbiota in an in vitro batch system.					
34623811	1	1	dep	energy	287:292	arg1	needs					294:298	needs	294:298	needs	294:298	A slow dietary fiber fermentation rate is desirable to obtain a steady metabolite release and even distribution throughout the entire colon, ensuring to meet the energy needs in the distal colon.					
34623811	5	2	theme	synergistic	791:801	arg1	effect					803:808	a synergistic effect	789:808	a synergistic effect on the gut microbiota composition	789:842	Moreover, the starch and chitosan composites induced a synergistic effect on the gut microbiota composition.					
34623811	1	3	theme	entire	252:257	arg1	colon					259:263	the entire colon	248:263	the entire colon	248:263	A slow dietary fiber fermentation rate is desirable to obtain a steady metabolite release and even distribution throughout the entire colon, ensuring to meet the energy needs in the distal colon.					
34623811	2	4	theme	human	486:490	arg1	microbiota					498:507	human fecal microbiota	486:507	human fecal microbiota in an in vitro batch system	486:535	In this study, we prepared starch-entrapped microspheres with a variable chitosan-to-starch ratio by means of electrospraying and investigated the fermentability by human fecal microbiota in an in vitro batch system.					
34623811	4	5	theme	chitosan	714:721	arg1	proportions					723:733	chitosan proportions	714:733	chitosan proportions	714:733	Butyrate production, in particular, gradually decreased with increasing chitosan proportions.					
34623811	7	6	theme	functional	1165:1174	arg1	modulation					1205:1214	functional and taxonomic gut microbiota modulation	1165:1214	functional and taxonomic gut microbiota modulation	1165:1214	Our findings highlight the possible benefits of rationally designing functional foods targeting functional and taxonomic gut microbiota modulation.					
34623811	0	7	theme	Gut	97:99	arg1	Composition					112:122	Human Gut Microbiota Composition	91:122	Human Gut Microbiota Composition	91:122	Starch Microspheres Entrapped with Chitosan Delay In Vitro Fecal Fermentation and Regulate Human Gut Microbiota Composition.					
34623811	7	8	theme	foods	1149:1153	arg1	benefits					1105:1112	the possible benefits	1092:1112	the possible benefits of rationally designing functional foods targeting functional and taxonomic gut microbiota modulation	1092:1214	Our findings highlight the possible benefits of rationally designing functional foods targeting functional and taxonomic gut microbiota modulation.					
34623811	3	9	theme	Starch	538:543	arg1	encapsulation					545:557	Starch encapsulation	538:557	Starch encapsulation	538:557	Starch encapsulation reduced microbial gas production and the concentration of short-chain fatty acids.					
34623811	0	10	theme	Human	91:95	arg1	Composition					112:122	Human Gut Microbiota Composition	91:122	Human Gut Microbiota Composition	91:122	Starch Microspheres Entrapped with Chitosan Delay In Vitro Fecal Fermentation and Regulate Human Gut Microbiota Composition.					
34623811	6	11	theme	chitosan/starch	1016:1030	arg1	microspheres					1032:1043	chitosan/starch microspheres	1016:1043	chitosan/starch microspheres	1016:1043	Roseburia, Lachnospiraceae, and Clostridiales were promoted by all of the microspheres, and the abundance of the aforementioned health-promoting taxa reached a maximum in chitosan/starch microspheres with a 1:6 (w/w) ratio.					
34623811	2	12	theme	in	515:516	arg1	system					530:535	an in vitro batch system	512:535	an in vitro batch system	512:535	In this study, we prepared starch-entrapped microspheres with a variable chitosan-to-starch ratio by means of electrospraying and investigated the fermentability by human fecal microbiota in an in vitro batch system.					
34623811	7	13	theme	designing	1128:1136	arg1	foods					1149:1153	rationally designing functional foods	1117:1153	rationally designing functional foods targeting functional and taxonomic gut microbiota modulation	1117:1214	Our findings highlight the possible benefits of rationally designing functional foods targeting functional and taxonomic gut microbiota modulation.					
34623811	4	14	theme	Butyrate	642:649	arg1	production					651:660	Butyrate production	642:660	Butyrate production	642:660	Butyrate production, in particular, gradually decreased with increasing chitosan proportions.					
34623811	0	15	theme	Microbiota	101:110	arg1	Composition					112:122	Human Gut Microbiota Composition	91:122	Human Gut Microbiota Composition	91:122	Starch Microspheres Entrapped with Chitosan Delay In Vitro Fecal Fermentation and Regulate Human Gut Microbiota Composition.					
34623811	7	16	theme	functional	1138:1147	arg1	foods					1149:1153	rationally designing functional foods	1117:1153	rationally designing functional foods targeting functional and taxonomic gut microbiota modulation	1117:1214	Our findings highlight the possible benefits of rationally designing functional foods targeting functional and taxonomic gut microbiota modulation.					
34623811	7	17	theme	taxonomic	1180:1188	arg1	modulation					1205:1214	functional and taxonomic gut microbiota modulation	1165:1214	functional and taxonomic gut microbiota modulation	1165:1214	Our findings highlight the possible benefits of rationally designing functional foods targeting functional and taxonomic gut microbiota modulation.					
34623811	3	18	theme	gas	577:579	arg1	production					581:590	microbial gas production	567:590	microbial gas production	567:590	Starch encapsulation reduced microbial gas production and the concentration of short-chain fatty acids.					
34623811	0	19	theme	Starch	0:5	arg1	Microspheres					7:18	Starch Microspheres	0:18	Starch Microspheres Entrapped with Chitosan	0:42	Starch Microspheres Entrapped with Chitosan Delay In Vitro Fecal Fermentation and Regulate Human Gut Microbiota Composition.					
34623811	2	20	theme	starch-entrapped	348:363	arg1	microspheres					365:376	starch-entrapped microspheres	348:376	starch-entrapped microspheres with a variable chitosan-to-starch ratio	348:417	In this study, we prepared starch-entrapped microspheres with a variable chitosan-to-starch ratio by means of electrospraying and investigated the fermentability by human fecal microbiota in an in vitro batch system.					
34623811	2	21	dep	in	515:516	arg1	vitro					518:522	vitro	518:522	vitro	518:522	In this study, we prepared starch-entrapped microspheres with a variable chitosan-to-starch ratio by means of electrospraying and investigated the fermentability by human fecal microbiota in an in vitro batch system.					
34623811	1	22	theme	steady	189:194	arg1	release					207:213	a steady metabolite release	187:213	a steady metabolite release	187:213	A slow dietary fiber fermentation rate is desirable to obtain a steady metabolite release and even distribution throughout the entire colon, ensuring to meet the energy needs in the distal colon.					
34623811	3	23	theme	microbial	567:575	arg1	production					581:590	microbial gas production	567:590	microbial gas production	567:590	Starch encapsulation reduced microbial gas production and the concentration of short-chain fatty acids.					
34623811	4	24	from	production	651:660	arg1	particular					666:675	particular	666:675	particular	666:675	Butyrate production, in particular, gradually decreased with increasing chitosan proportions.					
34623811	1	25	theme	metabolite	196:205	arg1	release					207:213	a steady metabolite release	187:213	a steady metabolite release	187:213	A slow dietary fiber fermentation rate is desirable to obtain a steady metabolite release and even distribution throughout the entire colon, ensuring to meet the energy needs in the distal colon.					
34623811	7	26	theme	microbiota	1194:1203	arg1	modulation					1205:1214	functional and taxonomic gut microbiota modulation	1165:1214	functional and taxonomic gut microbiota modulation	1165:1214	Our findings highlight the possible benefits of rationally designing functional foods targeting functional and taxonomic gut microbiota modulation.					
34623811	2	27	theme	chitosan-to-starch	394:411	arg1	ratio					413:417	a variable chitosan-to-starch ratio	383:417	a variable chitosan-to-starch ratio	383:417	In this study, we prepared starch-entrapped microspheres with a variable chitosan-to-starch ratio by means of electrospraying and investigated the fermentability by human fecal microbiota in an in vitro batch system.					
34623811	6	28	theme	taxa	990:993	arg1	abundance					941:949	the abundance	937:949	the abundance of the aforementioned health-promoting taxa	937:993	Roseburia, Lachnospiraceae, and Clostridiales were promoted by all of the microspheres, and the abundance of the aforementioned health-promoting taxa reached a maximum in chitosan/starch microspheres with a 1:6 (w/w) ratio.					
34623811	6	29	from	maximum	1005:1011	arg1	microspheres					1032:1043	chitosan/starch microspheres	1016:1043	chitosan/starch microspheres	1016:1043	Roseburia, Lachnospiraceae, and Clostridiales were promoted by all of the microspheres, and the abundance of the aforementioned health-promoting taxa reached a maximum in chitosan/starch microspheres with a 1:6 (w/w) ratio.					
34623811	7	30	theme	gut	1190:1192	arg1	modulation					1205:1214	functional and taxonomic gut microbiota modulation	1165:1214	functional and taxonomic gut microbiota modulation	1165:1214	Our findings highlight the possible benefits of rationally designing functional foods targeting functional and taxonomic gut microbiota modulation.					
34623811	0	31	dep	In	50:51	arg1	Vitro					53:57	Vitro	53:57	Vitro	53:57	Starch Microspheres Entrapped with Chitosan Delay In Vitro Fecal Fermentation and Regulate Human Gut Microbiota Composition.					
34623811	2	32	theme	variable	385:392	arg1	ratio					413:417	a variable chitosan-to-starch ratio	383:417	a variable chitosan-to-starch ratio	383:417	In this study, we prepared starch-entrapped microspheres with a variable chitosan-to-starch ratio by means of electrospraying and investigated the fermentability by human fecal microbiota in an in vitro batch system.					
34623811	5	33	theme	gut	817:819	arg1	composition					832:842	the gut microbiota composition	813:842	the gut microbiota composition	813:842	Moreover, the starch and chitosan composites induced a synergistic effect on the gut microbiota composition.					
34623811	2	34	theme	batch	524:528	arg1	system					530:535	an in vitro batch system	512:535	an in vitro batch system	512:535	In this study, we prepared starch-entrapped microspheres with a variable chitosan-to-starch ratio by means of electrospraying and investigated the fermentability by human fecal microbiota in an in vitro batch system.					
34623811	5	35	theme	microbiota	821:830	arg1	composition					832:842	the gut microbiota composition	813:842	the gut microbiota composition	813:842	Moreover, the starch and chitosan composites induced a synergistic effect on the gut microbiota composition.					
34623811	2	36	from	microbiota	498:507	arg1	system					530:535	an in vitro batch system	512:535	an in vitro batch system	512:535	In this study, we prepared starch-entrapped microspheres with a variable chitosan-to-starch ratio by means of electrospraying and investigated the fermentability by human fecal microbiota in an in vitro batch system.					
34623811	0	37	theme	In	50:51	arg1	Fermentation					65:76	In Vitro Fecal Fermentation	50:76	In Vitro Fecal Fermentation	50:76	Starch Microspheres Entrapped with Chitosan Delay In Vitro Fecal Fermentation and Regulate Human Gut Microbiota Composition.					
34623811	6	38	theme	aforementioned	958:971	arg1	taxa					990:993	the aforementioned health-promoting taxa	954:993	the aforementioned health-promoting taxa	954:993	Roseburia, Lachnospiraceae, and Clostridiales were promoted by all of the microspheres, and the abundance of the aforementioned health-promoting taxa reached a maximum in chitosan/starch microspheres with a 1:6 (w/w) ratio.					
34623811	7	39	theme	possible	1096:1103	arg1	benefits					1105:1112	the possible benefits	1092:1112	the possible benefits of rationally designing functional foods targeting functional and taxonomic gut microbiota modulation	1092:1214	Our findings highlight the possible benefits of rationally designing functional foods targeting functional and taxonomic gut microbiota modulation.					
34623811	1	40	theme	distal	307:312	arg1	colon					314:318	the distal colon	303:318	the distal colon	303:318	A slow dietary fiber fermentation rate is desirable to obtain a steady metabolite release and even distribution throughout the entire colon, ensuring to meet the energy needs in the distal colon.					
34623811	5	41	dep	starch	750:755	arg1	composites					770:779	composites	770:779	composites	770:779	Moreover, the starch and chitosan composites induced a synergistic effect on the gut microbiota composition.					
34623811	5	41	dep	starch	750:755	arg1	the					746:748	the	746:748	the	746:748	Moreover, the starch and chitosan composites induced a synergistic effect on the gut microbiota composition.					
34623811	5	42	from	effect	803:808	arg1	composition					832:842	the gut microbiota composition	813:842	the gut microbiota composition	813:842	Moreover, the starch and chitosan composites induced a synergistic effect on the gut microbiota composition.					
34623811	3	43	theme	acids	635:639	arg1	concentration					600:612	the concentration	596:612	the concentration of short-chain fatty acids	596:639	Starch encapsulation reduced microbial gas production and the concentration of short-chain fatty acids.					
34623811	3	43	theme	acids	635:639	arg1	production					581:590	microbial gas production	567:590	microbial gas production	567:590	Starch encapsulation reduced microbial gas production and the concentration of short-chain fatty acids.					
34623811	1	44	theme	slow	127:130	arg1	fiber					140:144	A slow dietary fiber	125:144	A slow dietary fiber fermentation rate	125:162	A slow dietary fiber fermentation rate is desirable to obtain a steady metabolite release and even distribution throughout the entire colon, ensuring to meet the energy needs in the distal colon.					
34623811	1	45	theme	even	219:222	arg1	distribution					224:235	even distribution	219:235	even distribution	219:235	A slow dietary fiber fermentation rate is desirable to obtain a steady metabolite release and even distribution throughout the entire colon, ensuring to meet the energy needs in the distal colon.					
34623811	1	46	theme	dietary	132:138	arg1	fiber					140:144	A slow dietary fiber	125:144	A slow dietary fiber fermentation rate	125:162	A slow dietary fiber fermentation rate is desirable to obtain a steady metabolite release and even distribution throughout the entire colon, ensuring to meet the energy needs in the distal colon.					
34623811	6	47	theme	w/w	1057:1059	arg1	ratio					1062:1066	a 1:6 (w/w) ratio	1050:1066	a 1:6 (w/w) ratio	1050:1066	Roseburia, Lachnospiraceae, and Clostridiales were promoted by all of the microspheres, and the abundance of the aforementioned health-promoting taxa reached a maximum in chitosan/starch microspheres with a 1:6 (w/w) ratio.					
34623811	3	48	theme	short-chain	617:627	arg1	acids					635:639	short-chain fatty acids	617:639	short-chain fatty acids	617:639	Starch encapsulation reduced microbial gas production and the concentration of short-chain fatty acids.					
34623811	1	49	theme	fiber	140:144	arg1	rate					159:162	A slow dietary fiber fermentation rate	125:162	A slow dietary fiber fermentation rate	125:162	A slow dietary fiber fermentation rate is desirable to obtain a steady metabolite release and even distribution throughout the entire colon, ensuring to meet the energy needs in the distal colon.					
34623811	0	50	theme	Fecal	59:63	arg1	Fermentation					65:76	In Vitro Fecal Fermentation	50:76	In Vitro Fecal Fermentation	50:76	Starch Microspheres Entrapped with Chitosan Delay In Vitro Fecal Fermentation and Regulate Human Gut Microbiota Composition.					
34623811	2	51	with	microspheres	365:376	arg1	ratio					413:417	a variable chitosan-to-starch ratio	383:417	a variable chitosan-to-starch ratio	383:417	In this study, we prepared starch-entrapped microspheres with a variable chitosan-to-starch ratio by means of electrospraying and investigated the fermentability by human fecal microbiota in an in vitro batch system.					
34623811	3	52	theme	fatty	629:633	arg1	acids					635:639	short-chain fatty acids	617:639	short-chain fatty acids	617:639	Starch encapsulation reduced microbial gas production and the concentration of short-chain fatty acids.					
34623811	1	53	theme	fermentation	146:157	arg1	rate					159:162	A slow dietary fiber fermentation rate	125:162	A slow dietary fiber fermentation rate	125:162	A slow dietary fiber fermentation rate is desirable to obtain a steady metabolite release and even distribution throughout the entire colon, ensuring to meet the energy needs in the distal colon.					
34623811	6	54	theme	health-promoting	973:988	arg1	taxa					990:993	the aforementioned health-promoting taxa	954:993	the aforementioned health-promoting taxa	954:993	Roseburia, Lachnospiraceae, and Clostridiales were promoted by all of the microspheres, and the abundance of the aforementioned health-promoting taxa reached a maximum in chitosan/starch microspheres with a 1:6 (w/w) ratio.					
34623811	6	55	theme	1:6	1052:1054	arg1	ratio					1062:1066	a 1:6 (w/w) ratio	1050:1066	a 1:6 (w/w) ratio	1050:1066	Roseburia, Lachnospiraceae, and Clostridiales were promoted by all of the microspheres, and the abundance of the aforementioned health-promoting taxa reached a maximum in chitosan/starch microspheres with a 1:6 (w/w) ratio.					
32504916	0	0	theme	Porphyra	84:91	arg1	haitanensis					93:103	Porphyra haitanensis	84:103	Porphyra haitanensis	84:103	Preparation, structural characterization, and bioactivity of PHPD-IV-4 derived from Porphyra haitanensis.					
32504916	5	1	theme	molecules	788:796	arg1	phosphorylation					753:767	the phosphorylation	749:767	the phosphorylation of MAPK signalling molecules	749:796	PHPD-IV-4 displayed the greatest biological activity by up-regulating the phosphorylation of MAPK signalling molecules.					
32504916	7	2	theme	6-anhydro-α-l-galactose	1007:1029	arg1	1→					1086:1087	6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→	1007:1087	1→	1086:1087	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	7	2	theme	6-anhydro-α-l-galactose	1007:1029	arg1	β-d-galactose					982:994	of → 3) β-d-galactose (1 → 4) 3	974:1004	of → 3) β-d-galactose (1 → 4) 3	974:1004	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	1	3	theme	haitanensis	163:173	arg1	source					203:208	an important food source	185:208	an important food source in many countries	185:226	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	3	theme	haitanensis	163:173	arg1	component					141:149	a major active component	126:149	a major active component	126:149	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	3	theme	haitanensis	163:173	arg1	Polysaccharides					106:120	Polysaccharides	106:120	Polysaccharides	106:120	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	2	4	theme	acid	447:450	arg1	system					452:457	the H2O2/ascorbic acid system	429:457	the H2O2/ascorbic acid system	429:457	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	8	5	theme	haitanensis	1190:1200	arg1	polysaccharides					1202:1216	P. haitanensis polysaccharides	1187:1216	P. haitanensis polysaccharides	1187:1216	This study provides a theoretical basis for the utilisation and structure-activity assessment of P. haitanensis polysaccharides.					
32504916	7	6	dep	β-d-galactose	982:994	arg1	of → 3					974:979	of → 3	974:979	of → 3	974:979	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	8	7	theme	P.	1187:1188	arg1	polysaccharides					1202:1216	P. haitanensis polysaccharides	1187:1216	P. haitanensis polysaccharides	1187:1216	This study provides a theoretical basis for the utilisation and structure-activity assessment of P. haitanensis polysaccharides.					
32504916	3	8	theme	immunostimulatory	514:530	arg1	activity					532:539	immunostimulatory activity	514:539	immunostimulatory activity	514:539	PHPD-IV elicited the highest level of antioxidant and immunostimulatory activity among the four fractions.					
32504916	8	9	theme	polysaccharides	1202:1216	arg1	assessment					1173:1182	structure-activity assessment	1154:1182	structure-activity assessment	1154:1182	This study provides a theoretical basis for the utilisation and structure-activity assessment of P. haitanensis polysaccharides.					
32504916	8	9	theme	polysaccharides	1202:1216	arg1	utilisation					1138:1148	utilisation	1138:1148	utilisation	1138:1148	This study provides a theoretical basis for the utilisation and structure-activity assessment of P. haitanensis polysaccharides.					
32504916	3	10	theme	antioxidant	498:508	arg1	level					489:493	the highest level	477:493	the highest level of antioxidant and immunostimulatory activity	477:539	PHPD-IV elicited the highest level of antioxidant and immunostimulatory activity among the four fractions.					
32504916	8	11	dep	utilisation	1138:1148	arg1	the					1134:1136	the	1134:1136	the	1134:1136	This study provides a theoretical basis for the utilisation and structure-activity assessment of P. haitanensis polysaccharides.					
32504916	8	12	theme	structure-activity	1154:1171	arg1	assessment					1173:1182	structure-activity assessment	1154:1182	structure-activity assessment	1154:1182	This study provides a theoretical basis for the utilisation and structure-activity assessment of P. haitanensis polysaccharides.					
32504916	1	13	theme	important	188:196	arg1	source					203:208	an important food source	185:208	an important food source in many countries	185:226	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	13	theme	important	188:196	arg1	component					141:149	a major active component	126:149	a major active component	126:149	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	13	theme	important	188:196	arg1	Polysaccharides					106:120	Polysaccharides	106:120	Polysaccharides	106:120	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	2	14	theme	haitanensis	380:390	arg1	polysaccharides					392:406	P. haitanensis polysaccharides	377:406	P. haitanensis polysaccharides	377:406	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	5	15	theme	greatest	703:710	arg1	activity					723:730	the greatest biological activity	699:730	the greatest biological activity	699:730	PHPD-IV-4 displayed the greatest biological activity by up-regulating the phosphorylation of MAPK signalling molecules.					
32504916	3	16	theme	activity	532:539	arg1	level					489:493	the highest level	477:493	the highest level of antioxidant and immunostimulatory activity	477:539	PHPD-IV elicited the highest level of antioxidant and immunostimulatory activity among the four fractions.					
32504916	1	17	theme	food	198:201	arg1	source					203:208	an important food source	185:208	an important food source in many countries	185:226	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	17	theme	food	198:201	arg1	component					141:149	a major active component	126:149	a major active component	126:149	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	17	theme	food	198:201	arg1	Polysaccharides					106:120	Polysaccharides	106:120	Polysaccharides	106:120	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	2	18	theme	P.	377:378	arg1	polysaccharides					392:406	P. haitanensis polysaccharides	377:406	P. haitanensis polysaccharides	377:406	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	5	19	theme	biological	712:721	arg1	activity					723:730	the greatest biological activity	699:730	the greatest biological activity	699:730	PHPD-IV-4 displayed the greatest biological activity by up-regulating the phosphorylation of MAPK signalling molecules.					
32504916	2	20	theme	H2O2/ascorbic	433:445	arg1	system					452:457	the H2O2/ascorbic acid system	429:457	the H2O2/ascorbic acid system	429:457	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	8	21	theme	theoretical	1112:1122	arg1	basis					1124:1128	a theoretical basis	1110:1128	a theoretical basis for the utilisation and structure-activity assessment of P. haitanensis polysaccharides	1110:1216	This study provides a theoretical basis for the utilisation and structure-activity assessment of P. haitanensis polysaccharides.					
32504916	6	22	theme	1/2D-NMR	901:908	arg1	analysis					910:917	1/2D-NMR analysis	901:917	1/2D-NMR analysis	901:917	PHPD-IV-4 was further purified, and its structure was characterized by monosaccharide composition and 1/2D-NMR analysis.					
32504916	2	23	theme	different	234:242	arg1	PHPD-III					316:323	PHPD-III	316:323	PHPD-III (128 kDa)	316:333	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	2	23	theme	different	234:242	arg1	fractions					261:269	Four different molecular-weight fractions	229:269	Four different molecular-weight fractions	229:269	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	2	23	theme	different	234:242	arg1	PHPD-I					279:284	PHPD-I	279:284	PHPD-I (329 kDa)	279:294	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	2	23	theme	different	234:242	arg1	PHPD-IV					340:346	PHPD-IV	340:346	PHPD-IV (10 kDa)	340:355	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	2	23	theme	different	234:242	arg1	PHPD-II					297:303	PHPD-II	297:303	PHPD-II (203 kDa)	297:313	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	0	24	theme	structural	13:22	arg1	characterization					24:39	structural characterization	13:39	structural characterization	13:39	Preparation, structural characterization, and bioactivity of PHPD-IV-4 derived from Porphyra haitanensis.					
32504916	1	25	theme	many	213:216	arg1	countries					218:226	many countries	213:226	many countries	213:226	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	5	26	theme	signalling	777:786	arg1	molecules					788:796	MAPK signalling molecules	772:796	MAPK signalling molecules	772:796	PHPD-IV-4 displayed the greatest biological activity by up-regulating the phosphorylation of MAPK signalling molecules.					
32504916	7	27	theme	β-d-galactose	1045:1057	arg1	1→					1086:1087	6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→	1007:1087	1→	1086:1087	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	7	27	theme	β-d-galactose	1045:1057	arg1	β-d-galactose					982:994	of → 3) β-d-galactose (1 → 4) 3	974:1004	of → 3) β-d-galactose (1 → 4) 3	974:1004	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	7	28	theme	α-l-galactose-6-S	1067:1083	arg1	1→					1086:1087	6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→	1007:1087	1→	1086:1087	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	7	28	theme	α-l-galactose-6-S	1067:1083	arg1	β-d-galactose					982:994	of → 3) β-d-galactose (1 → 4) 3	974:1004	of → 3) β-d-galactose (1 → 4) 3	974:1004	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	6	29	theme	monosaccharide	870:883	arg1	composition					885:895	monosaccharide composition	870:895	monosaccharide composition	870:895	PHPD-IV-4 was further purified, and its structure was characterized by monosaccharide composition and 1/2D-NMR analysis.					
32504916	1	30	theme	major	128:132	arg1	source					203:208	an important food source	185:208	an important food source in many countries	185:226	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	30	theme	major	128:132	arg1	component					141:149	a major active component	126:149	a major active component	126:149	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	30	theme	major	128:132	arg1	Polysaccharides					106:120	Polysaccharides	106:120	Polysaccharides	106:120	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	31	from	source	203:208	arg1	countries					218:226	many countries	213:226	many countries	213:226	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	32	theme	active	134:139	arg1	source					203:208	an important food source	185:208	an important food source in many countries	185:226	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	32	theme	active	134:139	arg1	component					141:149	a major active component	126:149	a major active component	126:149	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	1	32	theme	active	134:139	arg1	Polysaccharides					106:120	Polysaccharides	106:120	Polysaccharides	106:120	Polysaccharides are a major active component of Porphyra haitanensis, which is an important food source in many countries.					
32504916	7	33	theme	repeated	959:966	arg1	units					968:972	repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→	959:1087	repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→	959:1087	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	7	34	theme	1 → 4	1060:1064	arg1	1→					1086:1087	6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→	1007:1087	1→	1086:1087	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	7	34	theme	1 → 4	1060:1064	arg1	β-d-galactose					982:994	of → 3) β-d-galactose (1 → 4) 3	974:1004	of → 3) β-d-galactose (1 → 4) 3	974:1004	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	5	35	theme	MAPK	772:775	arg1	molecules					788:796	MAPK signalling molecules	772:796	MAPK signalling molecules	772:796	PHPD-IV-4 displayed the greatest biological activity by up-regulating the phosphorylation of MAPK signalling molecules.					
32504916	7	36	theme	1→	1032:1033	arg1	1→					1086:1087	6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→	1007:1087	1→	1086:1087	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	7	36	theme	1→	1032:1033	arg1	β-d-galactose					982:994	of → 3) β-d-galactose (1 → 4) 3	974:1004	of → 3) β-d-galactose (1 → 4) 3	974:1004	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	3	37	theme	highest	481:487	arg1	level					489:493	the highest level	477:493	the highest level of antioxidant and immunostimulatory activity	477:539	PHPD-IV elicited the highest level of antioxidant and immunostimulatory activity among the four fractions.					
32504916	7	38	dep	1→	1086:1087	arg1	and → 3					1036:1042	6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→	1007:1087	and → 3	1036:1042	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	0	39	theme	PHPD-IV-4	61:69	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, structural characterization, and bioactivity of PHPD-IV-4 derived from Porphyra haitanensis.					
32504916	0	39	theme	PHPD-IV-4	61:69	arg1	characterization					24:39	structural characterization	13:39	structural characterization	13:39	Preparation, structural characterization, and bioactivity of PHPD-IV-4 derived from Porphyra haitanensis.					
32504916	0	39	theme	PHPD-IV-4	61:69	arg1	bioactivity					46:56	bioactivity	46:56	bioactivity	46:56	Preparation, structural characterization, and bioactivity of PHPD-IV-4 derived from Porphyra haitanensis.					
32504916	2	40	theme	molecular-weight	244:259	arg1	PHPD-III					316:323	PHPD-III	316:323	PHPD-III (128 kDa)	316:333	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	2	40	theme	molecular-weight	244:259	arg1	fractions					261:269	Four different molecular-weight fractions	229:269	Four different molecular-weight fractions	229:269	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	2	40	theme	molecular-weight	244:259	arg1	PHPD-I					279:284	PHPD-I	279:284	PHPD-I (329 kDa)	279:294	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	2	40	theme	molecular-weight	244:259	arg1	PHPD-IV					340:346	PHPD-IV	340:346	PHPD-IV (10 kDa)	340:355	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	2	40	theme	molecular-weight	244:259	arg1	PHPD-II					297:303	PHPD-II	297:303	PHPD-II (203 kDa)	297:313	Four different molecular-weight fractions, namely PHPD-I (329 kDa), PHPD-II (203 kDa), PHPD-III (128 kDa), and PHPD-IV (10 kDa), were obtained from P. haitanensis polysaccharides by degradation using the H2O2/ascorbic acid system.					
32504916	7	41	dep	units	968:972	arg1	1→					1086:1087	6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→	1007:1087	1→	1086:1087	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	7	41	dep	units	968:972	arg1	β-d-galactose					982:994	of → 3) β-d-galactose (1 → 4) 3	974:1004	of → 3) β-d-galactose (1 → 4) 3	974:1004	The result revealed that PHPD-IV-4 was repeated units of → 3) β-d-galactose (1 → 4) 3, 6-anhydro-α-l-galactose (1→, and → 3) β-d-galactose (1 → 4) α-l-galactose-6-S (1→.					
32504916	4	42	theme	DEAE-cellulose	591:604	arg1	column					606:611	DEAE-cellulose column	591:611	DEAE-cellulose column	591:611	PHPD-IV was purified by DEAE-cellulose column and five fractions were obtained, designated PHPD-IV-1-PHPD-IV-5.					
32589090	12	0	theme	applicable	1396:1405	arg1	potential					1407:1415	an applicable potential	1393:1415	an applicable potential	1393:1415	These results indicated that XG-3 EPS had an applicable potential in food processing fields especially dairy products.					
32589090	4	1	theme	other	654:658	arg1	strains					679:685	other W. confusa species strains	654:685	other W. confusa species strains reported so far	654:701	This maximal value was far exceeding EPS production by other W. confusa species strains reported so far.					
32589090	3	2	theme	initial	475:481	arg1	L-1					470:472	sucrose 80.1 g L-1	455:472	sucrose 80.1 g L-1	455:472	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	2	theme	initial	475:481	arg1	L-1					515:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	1	3	theme	acid	231:234	arg1	bacteria					236:243	lactic acid bacteria	224:243	a lactic acid bacteria (LAB) Weissella confusa XG-3	222:272	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	1	3	theme	acid	231:234	arg1	LAB					246:248	LAB	246:248	LAB	246:248	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	11	4	theme	concentration-dependent	1319:1341	arg1	manner					1343:1348	a concentration-dependent manner	1317:1348	a concentration-dependent manner	1317:1348	This EPS efficiently coagulated sucrose-supplemented milk in a concentration-dependent manner.					
32589090	12	5	theme	food	1420:1423	arg1	fields					1436:1441	food processing fields	1420:1441	food processing fields especially dairy products	1420:1467	These results indicated that XG-3 EPS had an applicable potential in food processing fields especially dairy products.					
32589090	8	6	theme	negative	993:1000	arg1	impact					1002:1007	positive and negative impact	980:1007	positive and negative impact	980:1007	The EPS concentration and temperature exerted positive and negative impact on apparent viscosity, respectively.					
32589090	8	7	theme	EPS	938:940	arg1	concentration					942:954	The EPS concentration	934:954	The EPS concentration	934:954	The EPS concentration and temperature exerted positive and negative impact on apparent viscosity, respectively.					
32589090	3	8	theme	pH	483:484	arg1	L-1					470:472	sucrose 80.1 g L-1	455:472	sucrose 80.1 g L-1	455:472	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	8	theme	pH	483:484	arg1	L-1					515:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	1	9	theme	bacteria	236:243	arg1	XG-3					269:272	a lactic acid bacteria (LAB) Weissella confusa XG-3	222:272	a lactic acid bacteria (LAB) Weissella confusa XG-3	222:272	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	0	10	theme	rheological	98:108	arg1	property					110:117	its rheological property	94:117	its rheological property	94:117	The response surface optimization of exopolysaccharide produced by Weissella confusa XG-3 and its rheological property.					
32589090	8	11	theme	apparent	1012:1019	arg1	viscosity					1021:1029	apparent viscosity	1012:1029	apparent viscosity	1012:1029	The EPS concentration and temperature exerted positive and negative impact on apparent viscosity, respectively.					
32589090	3	12	theme	3.7 g	509:513	arg1	L-1					470:472	sucrose 80.1 g L-1	455:472	sucrose 80.1 g L-1	455:472	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	12	theme	3.7 g	509:513	arg1	L-1					515:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	2	13	dep	factors	343:349	arg1	sucrose					351:357	sucrose	351:357	sucrose	351:357	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	2	13	dep	factors	343:349	arg1	pH					368:369	initial pH	360:369	initial pH	360:369	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	2	13	dep	factors	343:349	arg1	acetate					382:388	sodium acetate	375:388	sodium acetate	375:388	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	2	13	dep	factors	343:349	arg1	factors					343:349	three significantly influencing factors	311:349	three significantly influencing factors sucrose, initial pH and sodium acetate	311:388	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	12	14	contain	had	1389:1391	arg2	potential					1407:1415	an applicable potential	1393:1415	an applicable potential	1393:1415	These results indicated that XG-3 EPS had an applicable potential in food processing fields especially dairy products.					
32589090	12	14	contain	had	1389:1391	arg1	EPS					1385:1387	XG-3 EPS	1380:1387	XG-3 EPS	1380:1387	These results indicated that XG-3 EPS had an applicable potential in food processing fields especially dairy products.					
32589090	4	15	theme	species	671:677	arg1	strains					679:685	other W. confusa species strains	654:685	other W. confusa species strains reported so far	654:701	This maximal value was far exceeding EPS production by other W. confusa species strains reported so far.					
32589090	3	16	theme	L-1	470:472	arg1	condition					442:450	the condition	438:450	the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield	438:540	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	7	17	theme	pseudo-plastic	909:922	arg1	behavior					924:931	pseudo-plastic behavior	909:931	pseudo-plastic behavior	909:931	It was a typical non-Newtonian fluid, exhibiting pseudo-plastic behavior.					
32589090	3	18	theme	sodium	494:499	arg1	acetate					501:507	sodium acetate	494:507	sodium acetate	494:507	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	10	19	theme	EPS	1237:1239	arg1	concentration					1241:1253	EPS concentration	1237:1253	EPS concentration	1237:1253	The intrinsic viscosity [η] was 409.7 (25 °C) and 201.7 (35 °C), which was relevant to temperature but irrelevant to EPS concentration.					
32589090	11	20	theme	sucrose-supplemented	1288:1307	arg1	milk					1309:1312	sucrose-supplemented milk	1288:1312	sucrose-supplemented milk	1288:1312	This EPS efficiently coagulated sucrose-supplemented milk in a concentration-dependent manner.					
32589090	3	21	theme	composite	399:407	arg1	CCD					417:419	Central composite design (CCD)	391:420	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield	391:540	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	22	theme	acetate	501:507	arg1	L-1					470:472	sucrose 80.1 g L-1	455:472	sucrose 80.1 g L-1	455:472	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	22	theme	acetate	501:507	arg1	L-1					515:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	2	23	theme	sodium	375:380	arg1	acetate					382:388	sodium acetate	375:388	sodium acetate	375:388	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	2	23	theme	sodium	375:380	arg1	factors					343:349	three significantly influencing factors	311:349	three significantly influencing factors sucrose, initial pH and sodium acetate	311:388	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	0	24	theme	response	4:11	arg1	optimization					21:32	The response surface optimization	0:32	The response surface optimization of exopolysaccharide	0:53	The response surface optimization of exopolysaccharide produced by Weissella confusa XG-3 and its rheological property.					
32589090	6	25	theme	EPS	830:832	arg1	properties					811:820	The rheological properties	795:820	The rheological properties of XG-3 EPS	795:832	The rheological properties of XG-3 EPS was further investigated.					
32589090	3	26	theme	maximal	524:530	arg1	L-1					470:472	sucrose 80.1 g L-1	455:472	sucrose 80.1 g L-1	455:472	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	26	theme	maximal	524:530	arg1	yield					536:540	the maximal EPS yield	520:540	the maximal EPS yield	520:540	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	12	27	theme	XG-3	1380:1383	arg1	EPS					1385:1387	XG-3 EPS	1380:1387	XG-3 EPS	1380:1387	These results indicated that XG-3 EPS had an applicable potential in food processing fields especially dairy products.					
32589090	7	28	theme	typical	869:875	arg1	fluid					891:895	a typical non-Newtonian fluid	867:895	a typical non-Newtonian fluid	867:895	It was a typical non-Newtonian fluid, exhibiting pseudo-plastic behavior.					
32589090	7	28	theme	typical	869:875	arg1	It					860:861	It	860:861	It	860:861	It was a typical non-Newtonian fluid, exhibiting pseudo-plastic behavior.					
32589090	2	29	theme	factorial	285:293	arg1	design					295:300	Two-level factorial design	275:300	Two-level factorial design	275:300	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	1	30	theme	Weissella	251:259	arg1	XG-3					269:272	a lactic acid bacteria (LAB) Weissella confusa XG-3	222:272	a lactic acid bacteria (LAB) Weissella confusa XG-3	222:272	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	7	31	theme	non-Newtonian	877:889	arg1	fluid					891:895	a typical non-Newtonian fluid	867:895	a typical non-Newtonian fluid	867:895	It was a typical non-Newtonian fluid, exhibiting pseudo-plastic behavior.					
32589090	7	31	theme	non-Newtonian	877:889	arg1	It					860:861	It	860:861	It	860:861	It was a typical non-Newtonian fluid, exhibiting pseudo-plastic behavior.					
32589090	2	32	theme	Two-level	275:283	arg1	design					295:300	Two-level factorial design	275:300	Two-level factorial design	275:300	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	9	33	theme	higher	1081:1086	arg1	viscosity					1088:1096	relatively higher viscosity	1070:1096	relatively higher viscosity	1070:1096	The XG-3 EPS maintained relatively higher viscosity at moderate pH (6-8).					
32589090	1	34	theme	exopolysaccharide	184:200	arg1	production					208:217	the exopolysaccharide (EPS) production	180:217	the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3	180:272	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	0	35	theme	surface	13:19	arg1	optimization					21:32	The response surface optimization	0:32	The response surface optimization of exopolysaccharide	0:53	The response surface optimization of exopolysaccharide produced by Weissella confusa XG-3 and its rheological property.					
32589090	1	36	theme	confusa	261:267	arg1	XG-3					269:272	a lactic acid bacteria (LAB) Weissella confusa XG-3	222:272	a lactic acid bacteria (LAB) Weissella confusa XG-3	222:272	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	6	37	theme	XG-3	825:828	arg1	EPS					830:832	XG-3 EPS	825:832	XG-3 EPS	825:832	The rheological properties of XG-3 EPS was further investigated.					
32589090	0	38	theme	exopolysaccharide	37:53	arg1	optimization					21:32	The response surface optimization	0:32	The response surface optimization of exopolysaccharide	0:53	The response surface optimization of exopolysaccharide produced by Weissella confusa XG-3 and its rheological property.					
32589090	4	39	theme	W.	660:661	arg1	strains					679:685	other W. confusa species strains	654:685	other W. confusa species strains reported so far	654:701	This maximal value was far exceeding EPS production by other W. confusa species strains reported so far.					
32589090	10	40	dep	409.7	1152:1156	arg1	relevant					1195:1202	relevant	1195:1202	relevant	1195:1202	The intrinsic viscosity [η] was 409.7 (25 °C) and 201.7 (35 °C), which was relevant to temperature but irrelevant to EPS concentration.					
32589090	10	40	dep	409.7	1152:1156	arg1	irrelevant					1223:1232	irrelevant	1223:1232	irrelevant	1223:1232	The intrinsic viscosity [η] was 409.7 (25 °C) and 201.7 (35 °C), which was relevant to temperature but irrelevant to EPS concentration.					
32589090	4	41	theme	maximal	604:610	arg1	value					612:616	This maximal value	599:616	This maximal value	599:616	This maximal value was far exceeding EPS production by other W. confusa species strains reported so far.					
32589090	3	42	theme	design	409:414	arg1	CCD					417:419	Central composite design (CCD)	391:420	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield	391:540	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	10	43	theme	intrinsic	1124:1132	arg1	viscosity					1134:1142	The intrinsic viscosity [η]	1120:1146	The intrinsic viscosity [η]	1120:1146	The intrinsic viscosity [η] was 409.7 (25 °C) and 201.7 (35 °C), which was relevant to temperature but irrelevant to EPS concentration.					
32589090	10	43	theme	intrinsic	1124:1132	arg1	409.7					1152:1156	409.7	1152:1156	409.7	1152:1156	The intrinsic viscosity [η] was 409.7 (25 °C) and 201.7 (35 °C), which was relevant to temperature but irrelevant to EPS concentration.					
32589090	3	44	theme	2.9-fold	553:560	arg1	increase					562:569	a 2.9-fold increase	551:569	a 2.9-fold increase	551:569	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	9	45	theme	moderate	1101:1108	arg1	6-8					1114:1116	6-8	1114:1116	6-8	1114:1116	The XG-3 EPS maintained relatively higher viscosity at moderate pH (6-8).					
32589090	9	45	theme	moderate	1101:1108	arg1	pH					1110:1111	moderate pH	1101:1111	moderate pH (6-8)	1101:1117	The XG-3 EPS maintained relatively higher viscosity at moderate pH (6-8).					
32589090	5	46	theme	large-scale	767:777	arg1	production					783:792	large-scale EPS production	767:792	large-scale EPS production	767:792	The results suggested that W. confusa XG-3 had a potential for large-scale EPS production.					
32589090	6	47	theme	rheological	799:809	arg1	properties					811:820	The rheological properties	795:820	The rheological properties of XG-3 EPS	795:832	The rheological properties of XG-3 EPS was further investigated.					
32589090	2	48	theme	influencing	331:341	arg1	sucrose					351:357	sucrose	351:357	sucrose	351:357	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	2	48	theme	influencing	331:341	arg1	pH					368:369	initial pH	360:369	initial pH	360:369	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	2	48	theme	influencing	331:341	arg1	acetate					382:388	sodium acetate	375:388	sodium acetate	375:388	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	2	48	theme	influencing	331:341	arg1	factors					343:349	three significantly influencing factors	311:349	three significantly influencing factors sucrose, initial pH and sodium acetate	311:388	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	3	49	theme	Central	391:397	arg1	CCD					417:419	Central composite design (CCD)	391:420	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield	391:540	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	5	50	contain	had	747:749	arg2	potential					753:761	a potential	751:761	a potential for large-scale EPS production	751:792	The results suggested that W. confusa XG-3 had a potential for large-scale EPS production.					
32589090	5	50	contain	had	747:749	arg1	confusa					734:740	confusa	734:740	confusa	734:740	The results suggested that W. confusa XG-3 had a potential for large-scale EPS production.					
32589090	1	51	theme	response	124:131	arg1	RSM					154:156	RSM	154:156	RSM	154:156	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	1	51	theme	response	124:131	arg1	methodology					141:151	The response surface methodology	120:151	The response surface methodology (RSM)	120:157	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	8	52	theme	positive	980:987	arg1	impact					1002:1007	positive and negative impact	980:1007	positive and negative impact	980:1007	The EPS concentration and temperature exerted positive and negative impact on apparent viscosity, respectively.					
32589090	4	53	theme	confusa	663:669	arg1	strains					679:685	other W. confusa species strains	654:685	other W. confusa species strains reported so far	654:701	This maximal value was far exceeding EPS production by other W. confusa species strains reported so far.					
32589090	12	54	theme	processing	1425:1434	arg1	fields					1436:1441	food processing fields	1420:1441	food processing fields especially dairy products	1420:1467	These results indicated that XG-3 EPS had an applicable potential in food processing fields especially dairy products.					
32589090	4	55	theme	EPS	636:638	arg1	production					640:649	EPS production	636:649	EPS production	636:649	This maximal value was far exceeding EPS production by other W. confusa species strains reported so far.					
32589090	1	56	theme	surface	133:139	arg1	RSM					154:156	RSM	154:156	RSM	154:156	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	1	56	theme	surface	133:139	arg1	methodology					141:151	The response surface methodology	120:151	The response surface methodology (RSM)	120:157	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	12	57	theme	dairy	1454:1458	arg1	products					1460:1467	dairy products	1454:1467	food processing fields especially dairy products	1420:1467	These results indicated that XG-3 EPS had an applicable potential in food processing fields especially dairy products.					
32589090	3	58	theme	97.5 ± 1.1 g	581:592	arg1	L-1					594:596	97.5 ± 1.1 g L-1	581:596	97.5 ± 1.1 g L-1	581:596	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	59	theme	80.1 g	463:468	arg1	L-1					470:472	sucrose 80.1 g L-1	455:472	sucrose 80.1 g L-1	455:472	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	59	theme	80.1 g	463:468	arg1	yield					536:540	the maximal EPS yield	520:540	the maximal EPS yield	520:540	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	59	theme	80.1 g	463:468	arg1	L-1					515:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	60	theme	sucrose	455:461	arg1	L-1					470:472	sucrose 80.1 g L-1	455:472	sucrose 80.1 g L-1	455:472	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	60	theme	sucrose	455:461	arg1	yield					536:540	the maximal EPS yield	520:540	the maximal EPS yield	520:540	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	60	theme	sucrose	455:461	arg1	L-1					515:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	initial pH 5.8 and sodium acetate 3.7 g L-1	475:517	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	0	61	theme	confusa	77:83	arg1	XG-3					85:88	Weissella confusa XG-3	67:88	Weissella confusa XG-3	67:88	The response surface optimization of exopolysaccharide produced by Weissella confusa XG-3 and its rheological property.					
32589090	5	62	theme	EPS	779:781	arg1	production					783:792	large-scale EPS production	767:792	large-scale EPS production	767:792	The results suggested that W. confusa XG-3 had a potential for large-scale EPS production.					
32589090	2	63	theme	initial	360:366	arg1	pH					368:369	initial pH	360:369	initial pH	360:369	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	2	63	theme	initial	360:366	arg1	factors					343:349	three significantly influencing factors	311:349	three significantly influencing factors sucrose, initial pH and sodium acetate	311:388	Two-level factorial design screened three significantly influencing factors sucrose, initial pH and sodium acetate.					
32589090	12	64	dep	fields	1436:1441	arg1	products					1460:1467	dairy products	1454:1467	food processing fields especially dairy products	1420:1467	These results indicated that XG-3 EPS had an applicable potential in food processing fields especially dairy products.					
32589090	0	65	theme	Weissella	67:75	arg1	XG-3					85:88	Weissella confusa XG-3	67:88	Weissella confusa XG-3	67:88	The response surface optimization of exopolysaccharide produced by Weissella confusa XG-3 and its rheological property.					
32589090	1	66	used	used	163:166	arg2	RSM					154:156	RSM	154:156	RSM	154:156	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	1	66	used	used	163:166	arg2	methodology					141:151	The response surface methodology	120:151	The response surface methodology (RSM)	120:157	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	3	67	theme	EPS	532:534	arg1	L-1					470:472	sucrose 80.1 g L-1	455:472	sucrose 80.1 g L-1	455:472	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	3	67	theme	EPS	532:534	arg1	yield					536:540	the maximal EPS yield	520:540	the maximal EPS yield	520:540	Central composite design (CCD) predicted under the condition of sucrose 80.1 g L-1, initial pH 5.8 and sodium acetate 3.7 g L-1, the maximal EPS yield obtained a 2.9-fold increase, reaching 97.5 ± 1.1 g L-1.					
32589090	1	68	theme	lactic	224:229	arg1	bacteria					236:243	lactic acid bacteria	224:243	a lactic acid bacteria (LAB) Weissella confusa XG-3	222:272	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32589090	1	68	theme	lactic	224:229	arg1	LAB					246:248	LAB	246:248	LAB	246:248	The response surface methodology (RSM) was used to optimize the exopolysaccharide (EPS) production by a lactic acid bacteria (LAB) Weissella confusa XG-3.					
32980415	7	0	theme	fibre	1267:1271	arg1	palm					1262:1265	raw fish tail palm fibre	1248:1271	raw fish tail palm fibre	1248:1271	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	2	1	theme	single	536:541	arg1	test					557:560	single fibre tensile test	536:560	single fibre tensile test	536:560	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	3	2	theme	Chemical	603:610	arg1	results					621:627	Chemical analysis results	603:627	Chemical analysis results	603:627	Chemical analysis results indicate that silane treatment improved the cellulose content of the fish tail palm fibre.					
32980415	4	3	theme	9	780:780	arg1	%					781:781	%	781:781	%	781:781	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	7	4	theme	tail	1257:1260	arg1	palm					1262:1265	raw fish tail palm fibre	1248:1271	raw fish tail palm fibre	1248:1271	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	4	5	theme	silane	783:788	arg1	palm					808:811	the 9% silane treated fish tail palm	776:811	the 9% silane treated fish tail palm fibre	776:817	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	6	6	theme	silane	1062:1067	arg1	fish					1077:1080	5% silane treated fish	1059:1080	5% silane treated fish tail palm fibre	1059:1096	FT-IR result indicates the removal of hemicellulose at characteristic wavelength of 1745 cm-1 for 5% silane treated fish tail palm fibre.					
32980415	2	7	dep	Fourier	487:493	arg1	Transform					495:503	Transform	495:503	Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM)	495:600	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	7	8	theme	raw	1248:1250	arg1	palm					1262:1265	raw fish tail palm fibre	1248:1271	raw fish tail palm fibre	1248:1271	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	6	9	theme	5	1059:1059	arg1	%					1060:1060	%	1060:1060	%	1060:1060	FT-IR result indicates the removal of hemicellulose at characteristic wavelength of 1745 cm-1 for 5% silane treated fish tail palm fibre.					
32980415	2	10	theme	tensile	549:555	arg1	test					557:560	single fibre tensile test	536:560	single fibre tensile test	536:560	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	6	11	theme	1745 cm-1	1045:1053	arg1	wavelength					1031:1040	characteristic wavelength	1016:1040	characteristic wavelength of 1745 cm-1	1016:1053	FT-IR result indicates the removal of hemicellulose at characteristic wavelength of 1745 cm-1 for 5% silane treated fish tail palm fibre.					
32980415	1	12	theme	tail	173:176	arg1	tree					183:186	the fish tail palm tree	164:186	the fish tail palm tree	164:186	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	7	13	theme	%	1216:1216	arg1	strength					1201:1208	an increased tensile strength	1180:1208	an increased tensile strength of 7.3%, 12%, and 6.6%	1180:1231	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	5	14	theme	X-ray	933:937	arg1	analysis					951:958	the X-ray diffraction analysis	929:958	the X-ray diffraction analysis	929:958	Also, it improved crystallinity index value of 62.5% for 5% silane treated fibre, which is confirmed through the X-ray diffraction analysis.					
32980415	1	15	theme	tree	183:186	arg1	portion					153:159	the peduncle portion	140:159	the peduncle portion of the fish tail palm tree	140:186	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	3	16	theme	tail	703:706	arg1	palm					708:711	the fish tail palm fibre	694:717	the fish tail palm fibre	694:717	Chemical analysis results indicate that silane treatment improved the cellulose content of the fish tail palm fibre.					
32980415	4	17	theme	fish	798:801	arg1	palm					808:811	the 9% silane treated fish tail palm	776:811	the 9% silane treated fish tail palm fibre	776:817	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	7	18	theme	tensile	1193:1199	arg1	strength					1201:1208	an increased tensile strength	1180:1208	an increased tensile strength of 7.3%, 12%, and 6.6%	1180:1231	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	1	19	theme	fibre	115:119	arg1	cellulosic					104:113	A novel cellulosic fibre	96:119	A novel cellulosic fibre	96:119	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	2	20	theme	InfraRed	505:512	arg1	FT-IR					528:532	FT-IR	528:532	FT-IR	528:532	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	2	20	theme	InfraRed	505:512	arg1	spectroscopy					514:525	InfraRed spectroscopy	505:525	InfraRed spectroscopy (FT-IR)	505:533	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	2	21	theme	fibres	423:428	arg1	chemical					337:344	chemical	337:344	chemical	337:344	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	3	22	theme	fibre	713:717	arg1	palm					708:711	the fish tail palm fibre	694:717	the fish tail palm fibre	694:717	Chemical analysis results indicate that silane treatment improved the cellulose content of the fish tail palm fibre.					
32980415	0	23	theme	treated	65:71	arg1	fibres					88:93	silane treated fish tail palm fibres	58:93	silane treated fish tail palm fibres	58:93	Characterization on chemical and mechanical properties of silane treated fish tail palm fibres.					
32980415	2	24	theme	tail	413:416	arg1	fibres					423:428	the extracted fish tail palm fibres	394:428	the extracted fish tail palm fibres	394:428	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	0	25	theme	tail	78:81	arg1	fibres					88:93	silane treated fish tail palm fibres	58:93	silane treated fish tail palm fibres	58:93	Characterization on chemical and mechanical properties of silane treated fish tail palm fibres.					
32980415	2	26	theme	chemical	456:463	arg1	Fourier					487:493	Fourier	487:493	Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM)	487:600	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	2	26	theme	chemical	456:463	arg1	analysis					477:484	chemical composition analysis	456:484	chemical composition analysis	456:484	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	0	27	from	Characterization	0:15	arg1	properties					44:53	chemical and mechanical properties	20:53	chemical and mechanical properties of silane treated fish tail palm fibres	20:93	Characterization on chemical and mechanical properties of silane treated fish tail palm fibres.					
32980415	2	28	theme	Electron	576:583	arg1	SEM					597:599	SEM	597:599	SEM	597:599	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	2	28	theme	Electron	576:583	arg1	Microscopy					585:594	Scanning Electron Microscopy	567:594	Scanning Electron Microscopy (SEM)	567:600	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	1	29	theme	novel	98:102	arg1	cellulosic					104:113	A novel cellulosic fibre	96:119	A novel cellulosic fibre	96:119	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	0	30	theme	fibres	88:93	arg1	properties					44:53	chemical and mechanical properties	20:53	chemical and mechanical properties of silane treated fish tail palm fibres	20:93	Characterization on chemical and mechanical properties of silane treated fish tail palm fibres.					
32980415	7	31	theme	%	1231:1231	arg1	strength					1201:1208	an increased tensile strength	1180:1208	an increased tensile strength of 7.3%, 12%, and 6.6%	1180:1231	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	1	32	theme	silane	289:294	arg1	solution					296:303	silane solution	289:303	silane solution	289:303	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	5	33	theme	%	871:871	arg1	value					858:862	crystallinity index value	838:862	crystallinity index value of 62.5% for 5% silane treated fibre, which is confirmed through the X-ray diffraction analysis	838:958	Also, it improved crystallinity index value of 62.5% for 5% silane treated fibre, which is confirmed through the X-ray diffraction analysis.					
32980415	2	34	theme	surface	374:380	arg1	property					382:389	surface property	374:389	surface property	374:389	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	8	35	theme	novel	1297:1301	arg1	fibres					1311:1316	novel natural fibres	1297:1316	novel natural fibres	1297:1316	Moreover, this type of novel natural fibres can reduce the cost while offering competent performance during the polymer-based product development.					
32980415	7	36	theme	Tensile	1099:1105	arg1	property					1107:1114	Tensile property	1099:1114	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%)	1099:1172	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	1	37	theme	different	244:252	arg1	concentrations					254:267	different concentrations	244:267	different concentrations (1%, 5%, and 9%) of silane solution	244:303	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	8	38	theme	fibres	1311:1316	arg1	type					1289:1292	this type	1284:1292	this type of novel natural fibres	1284:1316	Moreover, this type of novel natural fibres can reduce the cost while offering competent performance during the polymer-based product development.					
32980415	0	39	theme	chemical	20:27	arg1	properties					44:53	chemical and mechanical properties	20:53	chemical and mechanical properties of silane treated fish tail palm fibres	20:93	Characterization on chemical and mechanical properties of silane treated fish tail palm fibres.					
32980415	5	40	theme	index	852:856	arg1	value					858:862	crystallinity index value	838:862	crystallinity index value of 62.5% for 5% silane treated fibre, which is confirmed through the X-ray diffraction analysis	838:958	Also, it improved crystallinity index value of 62.5% for 5% silane treated fibre, which is confirmed through the X-ray diffraction analysis.					
32980415	2	41	theme	extracted	398:406	arg1	fibres					423:428	the extracted fish tail palm fibres	394:428	the extracted fish tail palm fibres	394:428	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	2	42	dep	chemical	337:344	arg1	mechanical					359:368	mechanical	359:368	mechanical	359:368	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	2	42	dep	chemical	337:344	arg1	functional					347:356	functional	347:356	functional	347:356	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	0	43	theme	mechanical	33:42	arg1	properties					44:53	chemical and mechanical properties	20:53	chemical and mechanical properties of silane treated fish tail palm fibres	20:93	Characterization on chemical and mechanical properties of silane treated fish tail palm fibres.					
32980415	2	44	from	analysis	325:332	arg1	chemical					337:344	chemical	337:344	chemical	337:344	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	2	44	from	analysis	325:332	arg1	property					382:389	surface property	374:389	surface property	374:389	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	6	45	theme	hemicellulose	999:1011	arg1	removal					988:994	the removal	984:994	the removal of hemicellulose	984:1011	FT-IR result indicates the removal of hemicellulose at characteristic wavelength of 1745 cm-1 for 5% silane treated fish tail palm fibre.					
32980415	1	46	theme	extracted	196:204	arg1	palm					216:219	the extracted fish tail palm fibre	192:225	the extracted fish tail palm fibre	192:225	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	5	47	theme	5	877:877	arg1	%					878:878	%	878:878	%	878:878	Also, it improved crystallinity index value of 62.5% for 5% silane treated fibre, which is confirmed through the X-ray diffraction analysis.					
32980415	7	48	theme	tail	1143:1146	arg1	palm					1148:1151	the silane treated fish tail palm	1119:1151	the silane treated fish tail palm fibre (1, 5, and 9%)	1119:1172	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	4	49	theme	highest	724:730	arg1	content					742:748	The highest cellulose content	720:748	The highest cellulose content of 72.51%	720:758	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	1	50	theme	tail	211:214	arg1	palm					216:219	the extracted fish tail palm fibre	192:225	the extracted fish tail palm fibre	192:225	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	7	51	theme	fibre	1153:1157	arg1	palm					1148:1151	the silane treated fish tail palm	1119:1151	the silane treated fish tail palm fibre (1, 5, and 9%)	1119:1172	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	7	52	theme	treated	1130:1136	arg1	palm					1148:1151	the silane treated fish tail palm	1119:1151	the silane treated fish tail palm fibre (1, 5, and 9%)	1119:1172	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	1	53	theme	fibre	221:225	arg1	palm					216:219	the extracted fish tail palm fibre	192:225	the extracted fish tail palm fibre	192:225	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	7	54	theme	fish	1252:1255	arg1	palm					1262:1265	raw fish tail palm fibre	1248:1271	raw fish tail palm fibre	1248:1271	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	6	55	theme	FT-IR	961:965	arg1	result					967:972	FT-IR result	961:972	FT-IR result	961:972	FT-IR result indicates the removal of hemicellulose at characteristic wavelength of 1745 cm-1 for 5% silane treated fish tail palm fibre.					
32980415	4	56	located	observed	764:771	arg2	content					742:748	The highest cellulose content	720:748	The highest cellulose content of 72.51%	720:758	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	4	56	located	observed	764:771	arg1	palm					808:811	the 9% silane treated fish tail palm	776:811	the 9% silane treated fish tail palm fibre	776:817	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	8	57	theme	product	1400:1406	arg1	development					1408:1418	the polymer-based product development	1382:1418	the polymer-based product development	1382:1418	Moreover, this type of novel natural fibres can reduce the cost while offering competent performance during the polymer-based product development.					
32980415	3	58	theme	analysis	612:619	arg1	results					621:627	Chemical analysis results	603:627	Chemical analysis results	603:627	Chemical analysis results indicate that silane treatment improved the cellulose content of the fish tail palm fibre.					
32980415	4	59	theme	%	781:781	arg1	palm					808:811	the 9% silane treated fish tail palm	776:811	the 9% silane treated fish tail palm fibre	776:817	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	6	60	theme	%	1060:1060	arg1	fish					1077:1080	5% silane treated fish	1059:1080	5% silane treated fish tail palm fibre	1059:1096	FT-IR result indicates the removal of hemicellulose at characteristic wavelength of 1745 cm-1 for 5% silane treated fish tail palm fibre.					
32980415	4	61	theme	%	758:758	arg1	content					742:748	The highest cellulose content	720:748	The highest cellulose content of 72.51%	720:758	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	3	62	theme	silane	643:648	arg1	treatment					650:658	silane treatment	643:658	silane treatment	643:658	Chemical analysis results indicate that silane treatment improved the cellulose content of the fish tail palm fibre.					
32980415	1	63	theme	fish	168:171	arg1	tree					183:186	the fish tail palm tree	164:186	the fish tail palm tree	164:186	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	2	64	theme	fibre	543:547	arg1	test					557:560	single fibre tensile test	536:560	single fibre tensile test	536:560	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	7	65	theme	%	1221:1221	arg1	strength					1201:1208	an increased tensile strength	1180:1208	an increased tensile strength of 7.3%, 12%, and 6.6%	1180:1231	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	1	66	theme	palm	178:181	arg1	tree					183:186	the fish tail palm tree	164:186	the fish tail palm tree	164:186	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	4	67	theme	fibre	813:817	arg1	palm					808:811	the 9% silane treated fish tail palm	776:811	the 9% silane treated fish tail palm fibre	776:817	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	6	68	theme	characteristic	1016:1029	arg1	wavelength					1031:1040	characteristic wavelength	1016:1040	characteristic wavelength of 1745 cm-1	1016:1053	FT-IR result indicates the removal of hemicellulose at characteristic wavelength of 1745 cm-1 for 5% silane treated fish tail palm fibre.					
32980415	3	69	theme	cellulose	673:681	arg1	content					683:689	the cellulose content	669:689	the cellulose content of the fish tail palm fibre	669:717	Chemical analysis results indicate that silane treatment improved the cellulose content of the fish tail palm fibre.					
32980415	5	70	theme	diffraction	939:949	arg1	analysis					951:958	the X-ray diffraction analysis	929:958	the X-ray diffraction analysis	929:958	Also, it improved crystallinity index value of 62.5% for 5% silane treated fibre, which is confirmed through the X-ray diffraction analysis.					
32980415	2	71	theme	composition	465:475	arg1	Fourier					487:493	Fourier	487:493	Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM)	487:600	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	2	71	theme	composition	465:475	arg1	analysis					477:484	chemical composition analysis	456:484	chemical composition analysis	456:484	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	3	72	theme	palm	708:711	arg1	content					683:689	the cellulose content	669:689	the cellulose content of the fish tail palm fibre	669:717	Chemical analysis results indicate that silane treatment improved the cellulose content of the fish tail palm fibre.					
32980415	1	73	dep	concentrations	254:267	arg1	%					283:283	9%	282:283	9%	282:283	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	1	73	dep	concentrations	254:267	arg1	%					271:271	1%	270:271	1%	270:271	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	1	73	dep	concentrations	254:267	arg1	%					275:275	5%	274:275	5%	274:275	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	4	74	theme	treated	790:796	arg1	palm					808:811	the 9% silane treated fish tail palm	776:811	the 9% silane treated fish tail palm fibre	776:817	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	6	75	theme	fibre	1092:1096	arg1	fish					1077:1080	5% silane treated fish	1059:1080	5% silane treated fish tail palm fibre	1059:1096	FT-IR result indicates the removal of hemicellulose at characteristic wavelength of 1745 cm-1 for 5% silane treated fish tail palm fibre.					
32980415	3	76	theme	fish	698:701	arg1	palm					708:711	the fish tail palm fibre	694:717	the fish tail palm fibre	694:717	Chemical analysis results indicate that silane treatment improved the cellulose content of the fish tail palm fibre.					
32980415	4	77	theme	tail	803:806	arg1	palm					808:811	the 9% silane treated fish tail palm	776:811	the 9% silane treated fish tail palm fibre	776:817	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	7	78	theme	increased	1183:1191	arg1	strength					1201:1208	an increased tensile strength	1180:1208	an increased tensile strength of 7.3%, 12%, and 6.6%	1180:1231	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	0	79	theme	fish	73:76	arg1	fibres					88:93	silane treated fish tail palm fibres	58:93	silane treated fish tail palm fibres	58:93	Characterization on chemical and mechanical properties of silane treated fish tail palm fibres.					
32980415	2	80	theme	palm	418:421	arg1	fibres					423:428	the extracted fish tail palm fibres	394:428	the extracted fish tail palm fibres	394:428	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	6	81	theme	treated	1069:1075	arg1	fish					1077:1080	5% silane treated fish	1059:1080	5% silane treated fish tail palm fibre	1059:1096	FT-IR result indicates the removal of hemicellulose at characteristic wavelength of 1745 cm-1 for 5% silane treated fish tail palm fibre.					
32980415	0	82	theme	palm	83:86	arg1	fibres					88:93	silane treated fish tail palm fibres	58:93	silane treated fish tail palm fibres	58:93	Characterization on chemical and mechanical properties of silane treated fish tail palm fibres.					
32980415	1	83	theme	peduncle	144:151	arg1	portion					153:159	the peduncle portion	140:159	the peduncle portion of the fish tail palm tree	140:186	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	2	84	theme	fish	408:411	arg1	fibres					423:428	the extracted fish tail palm fibres	394:428	the extracted fish tail palm fibres	394:428	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	2	85	theme	Scanning	567:574	arg1	SEM					597:599	SEM	597:599	SEM	597:599	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	2	85	theme	Scanning	567:574	arg1	Microscopy					585:594	Scanning Electron Microscopy	567:594	Scanning Electron Microscopy (SEM)	567:600	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	8	86	theme	natural	1303:1309	arg1	fibres					1311:1316	novel natural fibres	1297:1316	novel natural fibres	1297:1316	Moreover, this type of novel natural fibres can reduce the cost while offering competent performance during the polymer-based product development.					
32980415	2	87	theme	characteristic	310:323	arg1	analysis					325:332	The characteristic analysis	306:332	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres	306:428	The characteristic analysis on chemical, functional, mechanical and surface property of the extracted fish tail palm fibres were investigated through chemical composition analysis, Fourier Transform InfraRed spectroscopy (FT-IR), single fibre tensile test, and Scanning Electron Microscopy (SEM).					
32980415	1	88	theme	solution	296:303	arg1	concentrations					254:267	different concentrations	244:267	different concentrations (1%, 5%, and 9%) of silane solution	244:303	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	5	89	theme	crystallinity	838:850	arg1	value					858:862	crystallinity index value	838:862	crystallinity index value of 62.5% for 5% silane treated fibre, which is confirmed through the X-ray diffraction analysis	838:958	Also, it improved crystallinity index value of 62.5% for 5% silane treated fibre, which is confirmed through the X-ray diffraction analysis.					
32980415	4	90	theme	cellulose	732:740	arg1	content					742:748	The highest cellulose content	720:748	The highest cellulose content of 72.51%	720:758	The highest cellulose content of 72.51% was observed in the 9% silane treated fish tail palm fibre.					
32980415	0	91	theme	silane	58:63	arg1	fibres					88:93	silane treated fish tail palm fibres	58:93	silane treated fish tail palm fibres	58:93	Characterization on chemical and mechanical properties of silane treated fish tail palm fibres.					
32980415	7	92	theme	palm	1148:1151	arg1	property					1107:1114	Tensile property	1099:1114	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%)	1099:1172	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	8	93	theme	competent	1353:1361	arg1	performance					1363:1373	competent performance	1353:1373	competent performance	1353:1373	Moreover, this type of novel natural fibres can reduce the cost while offering competent performance during the polymer-based product development.					
32980415	7	94	theme	fish	1138:1141	arg1	palm					1148:1151	the silane treated fish tail palm	1119:1151	the silane treated fish tail palm fibre (1, 5, and 9%)	1119:1172	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	7	95	dep	fibre	1153:1157	arg1	%					1171:1171	1, 5, and 9%	1160:1171	1, 5, and 9%	1160:1171	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	1	96	theme	fish	206:209	arg1	palm					216:219	the extracted fish tail palm fibre	192:225	the extracted fish tail palm fibre	192:225	A novel cellulosic fibre was extracted from the peduncle portion of the fish tail palm tree and the extracted fish tail palm fibre was treated with different concentrations (1%, 5%, and 9%) of silane solution.					
32980415	7	97	theme	silane	1123:1128	arg1	palm					1148:1151	the silane treated fish tail palm	1119:1151	the silane treated fish tail palm fibre (1, 5, and 9%)	1119:1172	Tensile property of the silane treated fish tail palm fibre (1, 5, and 9%) shows an increased tensile strength of 7.3%, 12%, and 6.6% as compared to raw fish tail palm fibre.					
32980415	8	98	theme	polymer-based	1386:1398	arg1	development					1408:1418	the polymer-based product development	1382:1418	the polymer-based product development	1382:1418	Moreover, this type of novel natural fibres can reduce the cost while offering competent performance during the polymer-based product development.					
34870350	9	0	theme	complete	1961:1968	arg1	cell					1985:1988	a complete lithiated Si-S cell	1959:1988	a complete lithiated Si-S cell	1959:1988	As a result, an integration of the Si/C and S/C composites is proposed to achieve a complete lithiated Si-S cell.					
34870350	3	1	theme	environmental	682:694	arg1	benefits					696:703	some economical as well as environmental benefits	655:703	some economical as well as environmental benefits	655:703	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	3	2	from	synthesis	502:510	arg1	resources					550:558	CD resources	547:558	CD resources	547:558	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	9	3	theme	Si-S	1980:1983	arg1	cell					1985:1988	a complete lithiated Si-S cell	1959:1988	a complete lithiated Si-S cell	1959:1988	As a result, an integration of the Si/C and S/C composites is proposed to achieve a complete lithiated Si-S cell.					
34870350	2	4	theme	cone	314:317	arg1	structure					291:299	Their typical structure	277:299	Their typical structure of truncated cone	277:317	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	8	5	theme	Si/C	1819:1822	arg1	composite					1824:1832	our nanostructured Si/C composite	1800:1832	our nanostructured Si/C composite	1800:1832	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	2	6	theme	wide	330:333	arg1	molecules					352:360	guest molecules	346:360	guest molecules	346:360	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	2	6	theme	wide	330:333	arg1	variety					335:341	a wide variety	328:341	a wide variety of guest molecules	328:360	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	7	7	theme	different	1463:1471	arg1	electrolytes					1473:1484	different electrolytes	1463:1484	different electrolytes	1463:1484	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	7	8	theme	material	1451:1458	arg1	behavior					1435:1442	the behavior	1431:1442	the behavior of the material in different electrolytes	1431:1484	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	8	9	theme	LP30	1858:1861	arg1	electrolytes					1863:1874	LP30 electrolytes	1858:1874	LP30 electrolytes	1858:1874	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	4	10	theme	high-capacity	916:928	arg1	electrodes					930:939	high-capacity electrodes	916:939	high-capacity electrodes	916:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	4	10	theme	high-capacity	916:928	arg1	performance					901:911	satisfactory performance	888:911	satisfactory performance	888:911	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	8	11	theme	LP30	1522:1525	arg1	electrolyte					1527:1537	commercial LP30 electrolyte	1511:1537	commercial LP30 electrolyte	1511:1537	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	4	12	theme	simple	714:719	arg1	strategies					721:730	New and simple strategies	706:730	New and simple strategies	706:730	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	3	13	theme	effective	586:594	arg1	utilization					596:606	the effective utilization	582:606	the effective utilization of these products in the battery industry with some economical as well as environmental benefits	582:703	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	2	14	theme	typical	283:289	arg1	structure					291:299	Their typical structure	277:299	Their typical structure of truncated cone	277:317	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	8	15	theme	larger	1548:1553	arg1	capacity					1555:1562	a larger capacity	1546:1562	a larger capacity fade	1546:1567	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	8	16	dep	capacity	1750:1757	arg1	retention					1759:1767	retention	1759:1767	retention	1759:1767	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	7	17	theme	composite	1278:1286	arg1	area					1258:1261	the high surface area	1241:1261	the high surface area of the silicon composite	1241:1286	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	3	18	theme	products	617:624	arg1	utilization					596:606	the effective utilization	582:606	the effective utilization of these products in the battery industry with some economical as well as environmental benefits	582:703	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	8	19	theme	fade	1564:1567	arg1	capacity					1555:1562	a larger capacity	1546:1562	a larger capacity fade	1546:1567	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	4	20	theme	New	706:708	arg1	strategies					721:730	New and simple strategies	706:730	New and simple strategies	706:730	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	4	21	with	materials	816:824	arg1	nanosponges					865:875	carbonized hyper cross-linked CD (nanosponges)	831:876	carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes	831:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	3	22	from	resources	550:558	arg1	composites					531:540	active material composites	515:540	active material composites from CD resources	515:558	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	3	22	from	resources	550:558	arg1	synthesis					502:510	The synthesis	498:510	The synthesis of active material composites from CD resources	498:558	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	7	23	from	analysis	1419:1426	arg1	behavior					1435:1442	the behavior	1431:1442	the behavior of the material in different electrolytes	1431:1484	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	4	24	theme	silicon	787:793	arg1	materials					816:824	nanostructured silicon and sulfur composite materials	772:824	nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes	772:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	7	25	theme	high	1245:1248	arg1	area					1258:1261	the high surface area	1241:1261	the high surface area of the silicon composite	1241:1286	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	7	26	dep	dominate	1348:1355	arg1	opening					1400:1406	opening	1400:1406	opening	1400:1406	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	7	27	theme	detailed	1410:1417	arg1	analysis					1419:1426	a detailed analysis	1408:1426	a detailed analysis on the behavior of the material in different electrolytes	1408:1484	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	0	28	from	Nanosponges	43:53	arg1	Carbons					17:23	Micro-Mesoporous Carbons	0:23	Micro-Mesoporous Carbons from Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries.	0:141	Micro-Mesoporous Carbons from Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries.					
34870350	8	29	from	performance	1785:1795	arg1	electrolytes					1863:1874	LP30 electrolytes	1858:1874	LP30 electrolytes	1858:1874	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	8	29	from	performance	1785:1795	arg1	ether-based					1842:1852	ether-based	1842:1852	ether-based	1842:1852	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	0	30	theme	Micro-Mesoporous	0:15	arg1	Carbons					17:23	Micro-Mesoporous Carbons	0:23	Micro-Mesoporous Carbons from Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries.	0:141	Micro-Mesoporous Carbons from Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries.					
34870350	6	31	theme	silicon	1104:1110	arg1	nanoparticles					1112:1124	silicon nanoparticles	1104:1124	silicon nanoparticles	1104:1124	The embedding of silicon nanoparticles into the carbonized nanosponge allows to achieve high capacity and excellent cycling performance.					
34870350	8	32	theme	solid	1608:1612	arg1	formation					1642:1650	different solid electrolyte interface layer formation	1598:1650	different solid electrolyte interface layer formation	1598:1650	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	2	33	theme	unseen	420:425	arg1	CD					414:415	CD	414:415	CD	414:415	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	2	33	theme	unseen	420:425	arg1	components					427:436	unseen components	420:436	unseen components of food	420:444	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	3	34	theme	composites	531:540	arg1	synthesis					502:510	The synthesis	498:510	The synthesis of active material composites from CD resources	498:558	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	5	35	theme	carbon	981:986	arg1	host					988:991	the mesoporous carbon host	966:991	the mesoporous carbon host	966:991	For the sulfur cathode, the mesoporous carbon host limits polysulfide dissolution and shuttle effects and guarantees stable cycling performance.					
34870350	9	36	theme	Si/C	1912:1915	arg1	composites					1925:1934	the Si/C and S/C composites	1908:1934	composites	1925:1934	As a result, an integration of the Si/C and S/C composites is proposed to achieve a complete lithiated Si-S cell.					
34870350	8	37	theme	interface	1626:1634	arg1	formation					1642:1650	different solid electrolyte interface layer formation	1598:1650	different solid electrolyte interface layer formation	1598:1650	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	4	38	theme	carbonized	831:840	arg1	nanosponges					865:875	carbonized hyper cross-linked CD (nanosponges)	831:876	carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes	831:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	5	39	theme	stable	1059:1064	arg1	performance					1074:1084	stable cycling performance	1059:1084	stable cycling performance	1059:1084	For the sulfur cathode, the mesoporous carbon host limits polysulfide dissolution and shuttle effects and guarantees stable cycling performance.					
34870350	7	40	theme	overall	1361:1367	arg1	reversibility					1385:1397	the overall electrochemical reversibility	1357:1397	the overall electrochemical reversibility	1357:1397	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	9	41	theme	S/C	1921:1923	arg1	composites					1925:1934	the Si/C and S/C composites	1908:1934	composites	1925:1934	As a result, an integration of the Si/C and S/C composites is proposed to achieve a complete lithiated Si-S cell.					
34870350	4	42	theme	cross-linked	848:859	arg1	nanosponges					865:875	carbonized hyper cross-linked CD (nanosponges)	831:876	carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes	831:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	0	43	theme	High-Capacity	64:76	arg1	Silicon					78:84	High-Capacity Silicon Anodes and Sulfur Cathodes	64:111	Silicon	78:84	Micro-Mesoporous Carbons from Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries.					
34870350	4	44	theme	sulfur	799:804	arg1	materials					816:824	nanostructured silicon and sulfur composite materials	772:824	nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes	772:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	2	45	theme	guest	346:350	arg1	molecules					352:360	guest molecules	346:360	guest molecules	346:360	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	1	46	theme	enzymatic	245:253	arg1	conversion					255:264	enzymatic conversion	245:264	enzymatic conversion of starch	245:274	Manufactured globally on industrial scale, cyclodextrins (CD) are cyclic oligosaccharides produced by enzymatic conversion of starch.					
34870350	5	47	theme	shuttle	1028:1034	arg1	effects					1036:1042	shuttle effects	1028:1042	shuttle effects	1028:1042	For the sulfur cathode, the mesoporous carbon host limits polysulfide dissolution and shuttle effects and guarantees stable cycling performance.					
34870350	7	48	theme	electrode/electrolyte	1316:1336	arg1	interface					1338:1346	the electrode/electrolyte interface	1312:1346	the electrode/electrolyte interface	1312:1346	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	4	49	theme	materials	816:824	arg1	synthesis					759:767	the synthesis	755:767	the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes	755:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	3	50	theme	active	515:520	arg1	composites					531:540	active material composites	515:540	active material composites from CD resources	515:558	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	2	51	theme	inclusion	372:380	arg1	complexes					382:390	inclusion complexes	372:390	inclusion complexes	372:390	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	6	52	theme	carbonized	1135:1144	arg1	nanosponge					1146:1155	the carbonized nanosponge	1131:1155	the carbonized nanosponge	1131:1155	The embedding of silicon nanoparticles into the carbonized nanosponge allows to achieve high capacity and excellent cycling performance.					
34870350	9	53	theme	lithiated	1970:1978	arg1	cell					1985:1988	a complete lithiated Si-S cell	1959:1988	a complete lithiated Si-S cell	1959:1988	As a result, an integration of the Si/C and S/C composites is proposed to achieve a complete lithiated Si-S cell.					
34870350	8	54	theme	carbonate	1700:1708	arg1	addition					1710:1717	fluoroethylene carbonate addition	1685:1717	fluoroethylene carbonate addition	1685:1717	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	3	55	theme	CD	547:548	arg1	resources					550:558	CD resources	547:558	CD resources	547:558	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	8	56	theme	overall	1777:1783	arg1	performance					1785:1795	the overall performance	1773:1795	the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes	1773:1874	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	4	57	theme	satisfactory	888:899	arg1	electrodes					930:939	high-capacity electrodes	916:939	high-capacity electrodes	916:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	4	57	theme	satisfactory	888:899	arg1	performance					901:911	satisfactory performance	888:911	satisfactory performance	888:911	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	2	58	theme	truncated	304:312	arg1	cone					314:317	truncated cone	304:317	truncated cone	304:317	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	4	59	theme	CD	861:862	arg1	nanosponges					865:875	carbonized hyper cross-linked CD (nanosponges)	831:876	carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes	831:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	8	60	theme	nanostructured	1804:1817	arg1	composite					1824:1832	our nanostructured Si/C composite	1800:1832	our nanostructured Si/C composite	1800:1832	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	7	61	from	interface	1338:1346	arg1	characteristics					1293:1307	the characteristics	1289:1307	the characteristics at the electrode/electrolyte interface	1289:1346	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	3	62	with	industry	641:648	arg1	benefits					696:703	some economical as well as environmental benefits	655:703	some economical as well as environmental benefits	655:703	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	1	63	theme	cyclic	209:214	arg1	oligosaccharides					216:231	cyclic oligosaccharides	209:231	cyclic oligosaccharides produced by enzymatic conversion of starch	209:274	Manufactured globally on industrial scale, cyclodextrins (CD) are cyclic oligosaccharides produced by enzymatic conversion of starch.					
34870350	1	63	theme	cyclic	209:214	arg1	cyclodextrins					186:198	cyclodextrins	186:198	cyclodextrins (CD)	186:203	Manufactured globally on industrial scale, cyclodextrins (CD) are cyclic oligosaccharides produced by enzymatic conversion of starch.					
34870350	8	64	theme	commercial	1511:1520	arg1	electrolyte					1527:1537	commercial LP30 electrolyte	1511:1537	commercial LP30 electrolyte	1511:1537	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	6	65	theme	excellent	1193:1201	arg1	performance					1211:1221	excellent cycling performance	1193:1221	excellent cycling performance	1193:1221	The embedding of silicon nanoparticles into the carbonized nanosponge allows to achieve high capacity and excellent cycling performance.					
34870350	8	66	theme	electrolyte	1527:1537	arg1	use					1504:1506	the use	1500:1506	the use of commercial LP30 electrolyte	1500:1537	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	7	67	theme	surface	1250:1256	arg1	area					1258:1261	the high surface area	1241:1261	the high surface area of the silicon composite	1241:1286	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	5	68	theme	sulfur	950:955	arg1	cathode					957:963	the sulfur cathode	946:963	the sulfur cathode	946:963	For the sulfur cathode, the mesoporous carbon host limits polysulfide dissolution and shuttle effects and guarantees stable cycling performance.					
34870350	6	69	theme	cycling	1203:1209	arg1	performance					1211:1221	excellent cycling performance	1193:1221	excellent cycling performance	1193:1221	The embedding of silicon nanoparticles into the carbonized nanosponge allows to achieve high capacity and excellent cycling performance.					
34870350	8	70	theme	composite	1824:1832	arg1	capacity					1750:1757	the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes	1746:1874	capacity	1750:1757	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	8	70	theme	composite	1824:1832	arg1	performance					1785:1795	the overall performance	1773:1795	the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes	1773:1874	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	7	71	theme	silicon	1270:1276	arg1	composite					1278:1286	the silicon composite	1266:1286	the silicon composite	1266:1286	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	1	72	theme	industrial	168:177	arg1	scale					179:183	industrial scale	168:183	industrial scale	168:183	Manufactured globally on industrial scale, cyclodextrins (CD) are cyclic oligosaccharides produced by enzymatic conversion of starch.					
34870350	2	73	theme	pharmaceutical	471:484	arg1	CD					414:415	CD	414:415	CD	414:415	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	2	73	theme	pharmaceutical	471:484	arg1	excipients					486:495	pharmaceutical excipients	471:495	pharmaceutical excipients	471:495	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	0	74	theme	Lithiated	117:125	arg1	Batteries					132:140	Lithiated Si-S Batteries	117:140	Lithiated Si-S Batteries	117:140	Micro-Mesoporous Carbons from Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries.					
34870350	8	75	from	capacity	1750:1757	arg1	electrolytes					1863:1874	LP30 electrolytes	1858:1874	LP30 electrolytes	1858:1874	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	8	75	from	capacity	1750:1757	arg1	ether-based					1842:1852	ether-based	1842:1852	ether-based	1842:1852	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	5	76	theme	polysulfide	1000:1010	arg1	dissolution					1012:1022	polysulfide dissolution	1000:1022	polysulfide dissolution	1000:1022	For the sulfur cathode, the mesoporous carbon host limits polysulfide dissolution and shuttle effects and guarantees stable cycling performance.					
34870350	4	77	link	cross-linked	848:859	arg1	nanosponges					865:875	carbonized hyper cross-linked CD (nanosponges)	831:876	carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes	831:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	8	78	theme	different	1598:1606	arg1	formation					1642:1650	different solid electrolyte interface layer formation	1598:1650	different solid electrolyte interface layer formation	1598:1650	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	4	79	theme	nanostructured	772:785	arg1	silicon					787:793	nanostructured silicon	772:793	nanostructured silicon	772:793	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	7	80	from	behavior	1435:1442	arg1	electrolytes					1473:1484	different electrolytes	1463:1484	different electrolytes	1463:1484	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	6	81	theme	nanoparticles	1112:1124	arg1	embedding					1091:1099	The embedding	1087:1099	The embedding of silicon nanoparticles into the carbonized nanosponge	1087:1155	The embedding of silicon nanoparticles into the carbonized nanosponge allows to achieve high capacity and excellent cycling performance.					
34870350	2	82	theme	food	441:444	arg1	CD					414:415	CD	414:415	CD	414:415	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	2	82	theme	food	441:444	arg1	components					427:436	unseen components	420:436	unseen components of food	420:444	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	2	82	theme	food	441:444	arg1	textiles					458:465	textiles	458:465	textiles	458:465	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	2	82	theme	food	441:444	arg1	excipients					486:495	pharmaceutical excipients	471:495	pharmaceutical excipients	471:495	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	2	82	theme	food	441:444	arg1	cosmetics					447:455	cosmetics	447:455	cosmetics	447:455	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	8	83	theme	electrolyte	1614:1624	arg1	formation					1642:1650	different solid electrolyte interface layer formation	1598:1650	different solid electrolyte interface layer formation	1598:1650	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	0	84	theme	Cyclodextrin	30:41	arg1	Nanosponges					43:53	Cyclodextrin Nanosponges	30:53	Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries	30:140	Micro-Mesoporous Carbons from Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries.					
34870350	5	85	theme	mesoporous	970:979	arg1	host					988:991	the mesoporous carbon host	966:991	the mesoporous carbon host	966:991	For the sulfur cathode, the mesoporous carbon host limits polysulfide dissolution and shuttle effects and guarantees stable cycling performance.					
34870350	7	86	theme	electrochemical	1369:1383	arg1	reversibility					1385:1397	the overall electrochemical reversibility	1357:1397	the overall electrochemical reversibility	1357:1397	Moreover, due to the high surface area of the silicon composite, the characteristics at the electrode/electrolyte interface dominate the overall electrochemical reversibility, opening a detailed analysis on the behavior of the material in different electrolytes.					
34870350	8	87	theme	layer	1636:1640	arg1	formation					1642:1650	different solid electrolyte interface layer formation	1598:1650	different solid electrolyte interface layer formation	1598:1650	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	3	88	from	utilization	596:606	arg1	industry					641:648	the battery industry	629:648	the battery industry with some economical as well as environmental benefits	629:703	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	0	89	dep	Silicon	78:84	arg1	Anodes					86:91	Anodes	86:91	Anodes	86:91	Micro-Mesoporous Carbons from Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries.					
34870350	0	89	dep	Silicon	78:84	arg1	Cathodes					104:111	Cathodes	104:111	Cathodes	104:111	Micro-Mesoporous Carbons from Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries.					
34870350	3	90	theme	battery	633:639	arg1	industry					641:648	the battery industry	629:648	the battery industry with some economical as well as environmental benefits	629:703	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	0	91	theme	Si-S	127:130	arg1	Batteries					132:140	Lithiated Si-S Batteries	117:140	Lithiated Si-S Batteries	117:140	Micro-Mesoporous Carbons from Cyclodextrin Nanosponges Enabling High-Capacity Silicon Anodes and Sulfur Cathodes for Lithiated Si-S Batteries.					
34870350	5	92	theme	cycling	1066:1072	arg1	performance					1074:1084	stable cycling performance	1059:1084	stable cycling performance	1059:1084	For the sulfur cathode, the mesoporous carbon host limits polysulfide dissolution and shuttle effects and guarantees stable cycling performance.					
34870350	9	93	theme	composites	1925:1934	arg1	result					1882:1887	a result	1880:1887	a result	1880:1887	As a result, an integration of the Si/C and S/C composites is proposed to achieve a complete lithiated Si-S cell.					
34870350	9	93	theme	composites	1925:1934	arg1	integration					1893:1903	an integration	1890:1903	an integration of the Si/C and S/C composites	1890:1934	As a result, an integration of the Si/C and S/C composites is proposed to achieve a complete lithiated Si-S cell.					
34870350	4	94	theme	hyper	842:846	arg1	nanosponges					865:875	carbonized hyper cross-linked CD (nanosponges)	831:876	carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes	831:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	2	95	theme	molecules	352:360	arg1	molecules					352:360	guest molecules	346:360	guest molecules	346:360	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	2	95	theme	molecules	352:360	arg1	variety					335:341	a wide variety	328:341	a wide variety of guest molecules	328:360	Their typical structure of truncated cone can host a wide variety of guest molecules to create inclusion complexes; indeed, we daily use CD as unseen components of food, cosmetics, textiles and pharmaceutical excipients.					
34870350	6	96	theme	high	1175:1178	arg1	capacity					1180:1187	high capacity	1175:1187	high capacity	1175:1187	The embedding of silicon nanoparticles into the carbonized nanosponge allows to achieve high capacity and excellent cycling performance.					
34870350	3	97	theme	economical	660:669	arg1	benefits					696:703	some economical as well as environmental benefits	655:703	some economical as well as environmental benefits	655:703	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	8	98	theme	fluoroethylene	1685:1698	arg1	addition					1710:1717	fluoroethylene carbonate addition	1685:1717	fluoroethylene carbonate addition	1685:1717	We show that the use of commercial LP30 electrolyte causes a larger capacity fade, and this is associated with different solid electrolyte interface layer formation and it is also demonstrated that fluoroethylene carbonate addition can significantly increase the capacity retention and the overall performance of our nanostructured Si/C composite in both ether-based and LP30 electrolytes.					
34870350	4	99	theme	composite	806:814	arg1	materials					816:824	nanostructured silicon and sulfur composite materials	772:824	nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes	772:939	New and simple strategies are here presented for the synthesis of nanostructured silicon and sulfur composite materials with carbonized hyper cross-linked CD (nanosponges) that show satisfactory performance as high-capacity electrodes.					
34870350	3	100	theme	material	522:529	arg1	composites					531:540	active material composites	515:540	active material composites from CD resources	515:558	The synthesis of active material composites from CD resources can enable or enlarge the effective utilization of these products in the battery industry with some economical as well as environmental benefits.					
34870350	1	101	theme	starch	269:274	arg1	conversion					255:264	enzymatic conversion	245:264	enzymatic conversion of starch	245:274	Manufactured globally on industrial scale, cyclodextrins (CD) are cyclic oligosaccharides produced by enzymatic conversion of starch.					
33905364	7	0	theme	fluorescence	1060:1071	arg1	peaks					1073:1077	The fluorescence peaks	1056:1077	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration	1056:1129	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	8	1	theme	aeration	1341:1348	arg1	mode					1350:1353	aeration mode	1341:1353	aeration mode	1341:1353	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	4	2	theme	56.95	626:630	arg1	%					631:631	%	631:631	%	631:631	The proportion of TB-EPS decreased from 56.95% to 30.63%, and the proportion of LB-EPS increased obviously with the increase of oxygen aeration rate.					
33905364	1	3	theme	compositional	140:152	arg1	characteristics					154:168	compositional characteristics	140:168	compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR)	140:320	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	8	4	theme	treatment	1378:1386	arg1	process					1388:1394	the treatment process	1374:1394	the treatment process of activated sludge	1374:1414	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	7	5	with	LB-EPS	1087:1092	arg1	aeration					1122:1129	pure oxygen aeration	1110:1129	pure oxygen aeration	1110:1129	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	5	6	theme	polysaccharide	777:790	arg1	different					863:871	different	863:871	different	863:871	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	5	6	theme	polysaccharide	777:790	arg1	contents					740:747	The contents	736:747	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration	736:842	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	8	7	theme	sludge	1409:1414	arg1	process					1388:1394	the treatment process	1374:1394	the treatment process of activated sludge	1374:1414	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	5	8	with	polysaccharide	777:790	arg1	aeration					835:842	oxygen aeration	828:842	oxygen aeration	828:842	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	7	9	theme	humic	1173:1177	arg1	fractions					1189:1197	tryptophan PN-like and humic acid-like fractions	1150:1197	tryptophan PN-like and humic acid-like fractions	1150:1197	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	3	10	theme	most	484:487	arg1	sludge					504:509	most oxygen aerated sludge	484:509	most oxygen aerated sludge	484:509	The proportion of SMP of most oxygen aerated sludge was less than 10%, while that in air aerated sludge was as high as 30-40%.					
33905364	4	11	theme	LB-EPS	666:671	arg1	proportion					652:661	the proportion	648:661	the proportion of LB-EPS	648:671	The proportion of TB-EPS decreased from 56.95% to 30.63%, and the proportion of LB-EPS increased obviously with the increase of oxygen aeration rate.					
33905364	0	12	theme	batch	89:93	arg1	mode					95:98	batch mode	89:98	batch mode	89:98	Characteristics of the extracellular products of pure oxygen aerated activated sludge in batch mode.					
33905364	7	13	with	TB-EPS	1098:1103	arg1	aeration					1122:1129	pure oxygen aeration	1110:1129	pure oxygen aeration	1110:1129	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	3	14	theme	SMP	477:479	arg1	proportion					463:472	The proportion	459:472	The proportion of SMP of most oxygen aerated sludge	459:509	The proportion of SMP of most oxygen aerated sludge was less than 10%, while that in air aerated sludge was as high as 30-40%.					
33905364	3	14	theme	SMP	477:479	arg1	%					527:527	less than 10%	515:527	less than 10%	515:527	The proportion of SMP of most oxygen aerated sludge was less than 10%, while that in air aerated sludge was as high as 30-40%.					
33905364	4	15	theme	30.63	636:640	arg1	%					631:631	%	631:631	%	631:631	The proportion of TB-EPS decreased from 56.95% to 30.63%, and the proportion of LB-EPS increased obviously with the increase of oxygen aeration rate.					
33905364	8	16	theme	components	1246:1255	arg1	proportion					1228:1237	the proportion	1224:1237	the proportion of the components in the extracellular products	1224:1285	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	6	17	theme	zeta	925:928	arg1	potential					930:938	The zeta potential	921:938	The zeta potential of each component in activated sludge	921:976	The zeta potential of each component in activated sludge was negative, gradually decreasing with the progress of biological treatment.					
33905364	6	17	theme	zeta	925:928	arg1	negative					982:989	negative	982:989	negative	982:989	The zeta potential of each component in activated sludge was negative, gradually decreasing with the progress of biological treatment.					
33905364	3	18	theme	aerated	496:502	arg1	sludge					504:509	most oxygen aerated sludge	484:509	most oxygen aerated sludge	484:509	The proportion of SMP of most oxygen aerated sludge was less than 10%, while that in air aerated sludge was as high as 30-40%.					
33905364	7	19	theme	PN-like	1161:1167	arg1	fractions					1189:1197	tryptophan PN-like and humic acid-like fractions	1150:1197	tryptophan PN-like and humic acid-like fractions	1150:1197	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	5	20	theme	protein	756:762	arg1	different					863:871	different	863:871	different	863:871	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	5	20	theme	protein	756:762	arg1	contents					740:747	The contents	736:747	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration	736:842	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	1	21	theme	batch	339:343	arg1	mode					345:348	batch mode	339:348	batch mode	339:348	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	6	22	theme	activated	961:969	arg1	sludge					971:976	activated sludge	961:976	activated sludge	961:976	The zeta potential of each component in activated sludge was negative, gradually decreasing with the progress of biological treatment.					
33905364	3	23	theme	sludge	504:509	arg1	SMP					477:479	SMP	477:479	SMP of most oxygen aerated sludge	477:509	The proportion of SMP of most oxygen aerated sludge was less than 10%, while that in air aerated sludge was as high as 30-40%.					
33905364	1	24	theme	soluble	173:179	arg1	SMP					201:203	SMP	201:203	SMP	201:203	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	24	theme	soluble	173:179	arg1	products					191:198	soluble microbial products	173:198	soluble microbial products (SMP)	173:204	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	2	25	theme	aeration	423:430	arg1	methods					432:438	different aeration methods	413:438	different aeration methods	413:438	The structure of the extracellular products would change with different aeration methods or aeration rates.					
33905364	5	26	theme	products	814:821	arg1	polysaccharide					777:790	the polysaccharide	773:790	the polysaccharide (PS) of extracellular products with oxygen aeration	773:842	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	5	26	theme	products	814:821	arg1	PN					765:766	PN	765:766	PN	765:766	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	5	26	theme	products	814:821	arg1	PS					793:794	PS	793:794	PS	793:794	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	5	26	theme	products	814:821	arg1	protein					756:762	the protein	752:762	the protein (PN)	752:767	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	7	27	theme	pure	1110:1113	arg1	aeration					1122:1129	pure oxygen aeration	1110:1129	pure oxygen aeration	1110:1129	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	1	28	theme	microbial	181:189	arg1	SMP					201:203	SMP	201:203	SMP	201:203	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	28	theme	microbial	181:189	arg1	products					191:198	soluble microbial products	173:198	soluble microbial products (SMP)	173:204	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	2	29	theme	different	413:421	arg1	methods					432:438	different aeration methods	413:438	different aeration methods	413:438	The structure of the extracellular products would change with different aeration methods or aeration rates.					
33905364	1	30	theme	activated	258:266	arg1	sludge					268:273	the activated sludge	254:273	the activated sludge	254:273	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	31	from	effects	105:111	arg1	characteristics					154:168	compositional characteristics	140:168	compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR)	140:320	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	32	theme	products	191:198	arg1	characteristics					154:168	compositional characteristics	140:168	compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR)	140:320	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	0	33	theme	extracellular	23:35	arg1	products					37:44	the extracellular products	19:44	the extracellular products of pure oxygen	19:59	Characteristics of the extracellular products of pure oxygen aerated activated sludge in batch mode.					
33905364	1	34	theme	sludge	268:273	arg1	substances					234:243	extracellular polymeric substances	210:243	extracellular polymeric substances (EPS)	210:249	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	34	theme	sludge	268:273	arg1	SMP					201:203	SMP	201:203	SMP	201:203	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	34	theme	sludge	268:273	arg1	products					191:198	soluble microbial products	173:198	soluble microbial products (SMP)	173:204	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	34	theme	sludge	268:273	arg1	EPS					246:248	EPS	246:248	EPS	246:248	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	5	35	theme	extracellular	800:812	arg1	products					814:821	extracellular products	800:821	extracellular products	800:821	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	5	36	theme	oxygen	828:833	arg1	aeration					835:842	oxygen aeration	828:842	oxygen aeration	828:842	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	8	37	from	products	1278:1285	arg1	proportion					1228:1237	the proportion	1224:1237	the proportion of the components in the extracellular products	1224:1285	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	8	38	theme	activated	1399:1407	arg1	sludge					1409:1414	activated sludge	1399:1414	activated sludge	1399:1414	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	8	39	dep	rate	1332:1335	arg1	the					1319:1321	the	1319:1321	the	1319:1321	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	4	40	dep	%	631:631	arg1	to					633:634	to	633:634	to	633:634	The proportion of TB-EPS decreased from 56.95% to 30.63%, and the proportion of LB-EPS increased obviously with the increase of oxygen aeration rate.					
33905364	6	41	theme	treatment	1045:1053	arg1	progress					1022:1029	the progress	1018:1029	the progress of biological treatment	1018:1053	The zeta potential of each component in activated sludge was negative, gradually decreasing with the progress of biological treatment.					
33905364	2	42	theme	products	386:393	arg1	structure					355:363	The structure	351:363	The structure of the extracellular products	351:393	The structure of the extracellular products would change with different aeration methods or aeration rates.					
33905364	8	43	from	components	1246:1255	arg1	products					1278:1285	the extracellular products	1260:1285	the extracellular products	1260:1285	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	4	44	theme	aeration	721:728	arg1	rate					730:733	oxygen aeration rate	714:733	oxygen aeration rate	714:733	The proportion of TB-EPS decreased from 56.95% to 30.63%, and the proportion of LB-EPS increased obviously with the increase of oxygen aeration rate.					
33905364	2	45	theme	extracellular	372:384	arg1	products					386:393	the extracellular products	368:393	the extracellular products	368:393	The structure of the extracellular products would change with different aeration methods or aeration rates.					
33905364	0	46	theme	products	37:44	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics of the extracellular products of pure oxygen	0:59	Characteristics of the extracellular products of pure oxygen aerated activated sludge in batch mode.					
33905364	2	47	theme	aeration	443:450	arg1	rates					452:456	aeration rates	443:456	aeration rates	443:456	The structure of the extracellular products would change with different aeration methods or aeration rates.					
33905364	3	48	theme	air	544:546	arg1	sludge					556:561	air aerated sludge	544:561	air aerated sludge	544:561	The proportion of SMP of most oxygen aerated sludge was less than 10%, while that in air aerated sludge was as high as 30-40%.					
33905364	0	49	theme	oxygen	54:59	arg1	products					37:44	the extracellular products	19:44	the extracellular products of pure oxygen	19:59	Characteristics of the extracellular products of pure oxygen aerated activated sludge in batch mode.					
33905364	1	50	theme	sequential	291:300	arg1	SBR					317:319	SBR	317:319	SBR	317:319	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	50	theme	sequential	291:300	arg1	reactor					308:314	a sequential batch reactor	289:314	a sequential batch reactor (SBR)	289:320	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	0	51	from	aerated	61:67	arg1	mode					95:98	batch mode	89:98	batch mode	89:98	Characteristics of the extracellular products of pure oxygen aerated activated sludge in batch mode.					
33905364	6	52	theme	component	948:956	arg1	potential					930:938	The zeta potential	921:938	The zeta potential of each component in activated sludge	921:976	The zeta potential of each component in activated sludge was negative, gradually decreasing with the progress of biological treatment.					
33905364	6	52	theme	component	948:956	arg1	negative					982:989	negative	982:989	negative	982:989	The zeta potential of each component in activated sludge was negative, gradually decreasing with the progress of biological treatment.					
33905364	7	53	theme	oxygen	1115:1120	arg1	aeration					1122:1129	pure oxygen aeration	1110:1129	pure oxygen aeration	1110:1129	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	0	54	theme	pure	49:52	arg1	oxygen					54:59	pure oxygen	49:59	pure oxygen	49:59	Characteristics of the extracellular products of pure oxygen aerated activated sludge in batch mode.					
33905364	1	55	theme	batch	302:306	arg1	SBR					317:319	SBR	317:319	SBR	317:319	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	55	theme	batch	302:306	arg1	reactor					308:314	a sequential batch reactor	289:314	a sequential batch reactor (SBR)	289:320	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	8	56	theme	aeration	1323:1330	arg1	rate					1332:1335	aeration rate	1323:1335	aeration rate	1323:1335	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	4	57	theme	rate	730:733	arg1	increase					702:709	the increase	698:709	the increase of oxygen aeration rate	698:733	The proportion of TB-EPS decreased from 56.95% to 30.63%, and the proportion of LB-EPS increased obviously with the increase of oxygen aeration rate.					
33905364	7	58	theme	acid-like	1179:1187	arg1	fractions					1189:1197	tryptophan PN-like and humic acid-like fractions	1150:1197	tryptophan PN-like and humic acid-like fractions	1150:1197	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	7	59	with	SMP	1082:1084	arg1	aeration					1122:1129	pure oxygen aeration	1110:1129	pure oxygen aeration	1110:1129	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	5	60	with	protein	756:762	arg1	aeration					835:842	oxygen aeration	828:842	oxygen aeration	828:842	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	5	61	theme	p	910:910	arg1	<					912:912	p < 0.05	910:917	p < 0.05	910:917	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	5	61	theme	p	910:910	arg1	PS					906:907	PS	906:907	PS (p < 0.05)	906:918	The contents of the protein (PN) and the polysaccharide (PS) of extracellular products with oxygen aeration were significantly different, and the PN was much higher than PS (p < 0.05).					
33905364	8	62	theme	extracellular	1264:1276	arg1	products					1278:1285	the extracellular products	1260:1285	the extracellular products	1260:1285	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	4	63	theme	oxygen	714:719	arg1	rate					730:733	oxygen aeration rate	714:733	oxygen aeration rate	714:733	The proportion of TB-EPS decreased from 56.95% to 30.63%, and the proportion of LB-EPS increased obviously with the increase of oxygen aeration rate.					
33905364	1	64	theme	pure	116:119	arg1	aeration					128:135	pure oxygen aeration	116:135	pure oxygen aeration	116:135	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	65	theme	extracellular	210:222	arg1	substances					234:243	extracellular polymeric substances	210:243	extracellular polymeric substances (EPS)	210:249	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	65	theme	extracellular	210:222	arg1	EPS					246:248	EPS	246:248	EPS	246:248	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	0	66	from	mode	95:98	arg1	aerated					61:67	aerated	61:67	aerated	61:67	Characteristics of the extracellular products of pure oxygen aerated activated sludge in batch mode.					
33905364	1	67	theme	oxygen	121:126	arg1	aeration					128:135	pure oxygen aeration	116:135	pure oxygen aeration	116:135	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	68	theme	polymeric	224:232	arg1	substances					234:243	extracellular polymeric substances	210:243	extracellular polymeric substances (EPS)	210:249	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	1	68	theme	polymeric	224:232	arg1	EPS					246:248	EPS	246:248	EPS	246:248	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	0	69	theme	activated	69:77	arg1	sludge					79:84	activated sludge	69:84	activated sludge	69:84	Characteristics of the extracellular products of pure oxygen aerated activated sludge in batch mode.					
33905364	3	70	theme	aerated	548:554	arg1	sludge					556:561	air aerated sludge	544:561	air aerated sludge	544:561	The proportion of SMP of most oxygen aerated sludge was less than 10%, while that in air aerated sludge was as high as 30-40%.					
33905364	6	71	from	potential	930:938	arg1	sludge					971:976	activated sludge	961:976	activated sludge	961:976	The zeta potential of each component in activated sludge was negative, gradually decreasing with the progress of biological treatment.					
33905364	1	72	theme	aeration	128:135	arg1	effects					105:111	The effects	101:111	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR)	101:320	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	4	73	theme	TB-EPS	604:609	arg1	proportion					590:599	The proportion	586:599	The proportion of TB-EPS	586:609	The proportion of TB-EPS decreased from 56.95% to 30.63%, and the proportion of LB-EPS increased obviously with the increase of oxygen aeration rate.					
33905364	1	74	theme	substances	234:243	arg1	characteristics					154:168	compositional characteristics	140:168	compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR)	140:320	The effects of pure oxygen aeration on compositional characteristics of soluble microbial products (SMP) and extracellular polymeric substances (EPS) of the activated sludge acclimated in a sequential batch reactor (SBR) were explored in batch mode.					
33905364	6	75	theme	biological	1034:1043	arg1	treatment					1045:1053	biological treatment	1034:1053	biological treatment	1034:1053	The zeta potential of each component in activated sludge was negative, gradually decreasing with the progress of biological treatment.					
33905364	3	76	theme	oxygen	489:494	arg1	sludge					504:509	most oxygen aerated sludge	484:509	most oxygen aerated sludge	484:509	The proportion of SMP of most oxygen aerated sludge was less than 10%, while that in air aerated sludge was as high as 30-40%.					
33905364	8	77	from	proportion	1228:1237	arg1	products					1278:1285	the extracellular products	1260:1285	the extracellular products	1260:1285	The results showed that the proportion of the components in the extracellular products could be regulated by adjusting the aeration rate and aeration mode, so as to optimize the treatment process of activated sludge.					
33905364	7	78	from	peaks	1073:1077	arg1	TB-EPS					1098:1103	TB-EPS	1098:1103	TB-EPS	1098:1103	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	7	78	from	peaks	1073:1077	arg1	LB-EPS					1087:1092	LB-EPS	1087:1092	LB-EPS	1087:1092	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
33905364	7	78	from	peaks	1073:1077	arg1	SMP					1082:1084	SMP	1082:1084	SMP	1082:1084	The fluorescence peaks in SMP, LB-EPS and TB-EPS with pure oxygen aeration were attributed to tryptophan PN-like and humic acid-like fractions.					
34662794	3	0	dep	Pleurotus	401:409	arg1	eryngii					411:417	eryngii	411:417	eryngii	411:417	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	3	1	from	microbiota	462:471	arg1	characteristics					382:396	the digestion and fermentation characteristics	351:396	characteristics	382:396	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	4	2	theme	PEP	601:603	arg1	structure					588:596	the overall structure	576:596	the overall structure of PEP	576:603	The results showed that the molecular weight of PEP remained unchanged after simulated digestion, and the overall structure of PEP was not destroyed, indicating that PEP was not decomposed during digestion.					
34662794	2	3	located	found	238:242	arg2	polysaccharides					222:236	The polysaccharides	218:236	The polysaccharides found in this mushroom	218:259	The polysaccharides found in this mushroom are strong bioactive.					
34662794	2	3	located	found	238:242	arg1	mushroom					252:259	this mushroom	247:259	this mushroom	247:259	The polysaccharides found in this mushroom are strong bioactive.					
34662794	2	3	located	found	238:242	arg2	bioactive					272:280	bioactive	272:280	bioactive	272:280	The polysaccharides found in this mushroom are strong bioactive.					
34662794	3	4	from	effect	448:453	arg1	microbiota					462:471	gut microbiota	458:471	gut microbiota	458:471	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	5	5	used	utilized	732:739	arg2	PEP					711:713	PEP	711:713	PEP	711:713	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	6	6	theme	Firmicutes	956:965	arg1	abundance					943:951	the relative abundance	930:951	the relative abundance of Firmicutes	930:965	Meanwhile, PEP regulated the composition of intestinal flora, and the relative abundance of Firmicutes increased significantly.					
34662794	5	7	theme	intestinal	744:753	arg1	flora					755:759	intestinal flora	744:759	intestinal flora	744:759	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	5	8	theme	fecal	848:852	arg1	cultures					854:861	fecal cultures	848:861	fecal cultures	848:861	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	3	9	theme	gut	458:460	arg1	microbiota					462:471	gut microbiota	458:471	gut microbiota	458:471	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	3	10	theme	fermentation	369:380	arg1	characteristics					382:396	the digestion and fermentation characteristics	351:396	characteristics	382:396	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	3	11	from	polysaccharide	419:432	arg1	microbiota					462:471	gut microbiota	458:471	gut microbiota	458:471	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	1	12	theme	large	196:200	arg1	scale					202:206	a large scale	194:206	a large scale in China	194:215	Pleurotus eryngii is a valuable new edible mushroom variety cultivated on a large scale in China.					
34662794	3	13	theme	polysaccharide	419:432	arg1	characteristics					382:396	the digestion and fermentation characteristics	351:396	characteristics	382:396	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	0	14	from	Pleurotus	40:48	arg1	Characterization					0:15	Characterization	0:15	Characterization of polysaccharide from Pleurotus eryngii during simulated gastrointestinal digestion and fermentation.	0:118	Characterization of polysaccharide from Pleurotus eryngii during simulated gastrointestinal digestion and fermentation.					
34662794	3	15	theme	effect	448:453	arg1	characteristics					382:396	the digestion and fermentation characteristics	351:396	characteristics	382:396	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	7	16	theme	intestinal	1060:1069	arg1	health					1071:1076	intestinal health	1060:1076	intestinal health	1060:1076	These suggests that PEP can be used as a functional food to promote intestinal health and prevent disease.					
34662794	3	17	theme	Pleurotus	401:409	arg1	polysaccharide					419:432	Pleurotus eryngii polysaccharide	401:432	Pleurotus eryngii polysaccharide (PEP)	401:438	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	3	17	theme	Pleurotus	401:409	arg1	PEP					435:437	PEP	435:437	PEP	435:437	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	5	18	theme	fatty	797:801	arg1	SCFAs					810:814	SCFAs	810:814	SCFAs	810:814	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	5	18	theme	fatty	797:801	arg1	acids					803:807	short-chain fatty acids	785:807	short-chain fatty acids (SCFAs)	785:815	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	3	19	used	used	294:297	arg2	study					288:292	This study	283:292	This study	283:292	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	6	20	theme	relative	934:941	arg1	abundance					943:951	the relative abundance	930:951	the relative abundance of Firmicutes	930:965	Meanwhile, PEP regulated the composition of intestinal flora, and the relative abundance of Firmicutes increased significantly.					
34662794	3	21	theme	simulated	299:307	arg1	digestion					309:317	simulated digestion	299:317	simulated digestion	299:317	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	4	22	theme	overall	580:586	arg1	structure					588:596	the overall structure	576:596	the overall structure of PEP	576:603	The results showed that the molecular weight of PEP remained unchanged after simulated digestion, and the overall structure of PEP was not destroyed, indicating that PEP was not decomposed during digestion.					
34662794	7	23	used	used	1023:1026	arg2	PEP					1012:1014	PEP	1012:1014	PEP	1012:1014	These suggests that PEP can be used as a functional food to promote intestinal health and prevent disease.					
34662794	7	23	used	used	1023:1026	arg2	food					1044:1047	a functional food	1031:1047	a functional food to promote intestinal health and prevent disease	1031:1096	These suggests that PEP can be used as a functional food to promote intestinal health and prevent disease.					
34662794	5	24	theme	short-chain	785:795	arg1	SCFAs					810:814	SCFAs	810:814	SCFAs	810:814	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	5	24	theme	short-chain	785:795	arg1	acids					803:807	short-chain fatty acids	785:807	short-chain fatty acids (SCFAs)	785:815	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	4	25	theme	simulated	551:559	arg1	digestion					561:569	simulated digestion	551:569	simulated digestion	551:569	The results showed that the molecular weight of PEP remained unchanged after simulated digestion, and the overall structure of PEP was not destroyed, indicating that PEP was not decomposed during digestion.					
34662794	7	26	theme	functional	1033:1042	arg1	food					1044:1047	a functional food	1031:1047	a functional food to promote intestinal health and prevent disease	1031:1096	These suggests that PEP can be used as a functional food to promote intestinal health and prevent disease.					
34662794	7	26	theme	functional	1033:1042	arg1	PEP					1012:1014	PEP	1012:1014	PEP	1012:1014	These suggests that PEP can be used as a functional food to promote intestinal health and prevent disease.					
34662794	3	27	from	characteristics	382:396	arg1	microbiota					462:471	gut microbiota	458:471	gut microbiota	458:471	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	5	28	theme	pH	836:837	arg1	value					839:843	the pH value	832:843	the pH value in fecal cultures	832:861	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	0	29	theme	simulated	65:73	arg1	digestion					92:100	simulated gastrointestinal digestion	65:100	simulated gastrointestinal digestion	65:100	Characterization of polysaccharide from Pleurotus eryngii during simulated gastrointestinal digestion and fermentation.					
34662794	4	30	theme	molecular	502:510	arg1	weight					512:517	the molecular weight	498:517	the molecular weight of PEP	498:524	The results showed that the molecular weight of PEP remained unchanged after simulated digestion, and the overall structure of PEP was not destroyed, indicating that PEP was not decomposed during digestion.					
34662794	3	31	theme	fermentation	323:334	arg1	model					336:340	fermentation model	323:340	fermentation model	323:340	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	0	32	theme	gastrointestinal	75:90	arg1	digestion					92:100	simulated gastrointestinal digestion	65:100	simulated gastrointestinal digestion	65:100	Characterization of polysaccharide from Pleurotus eryngii during simulated gastrointestinal digestion and fermentation.					
34662794	5	33	theme	acids	803:807	arg1	SCFAs					810:814	SCFAs	810:814	SCFAs	810:814	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	5	33	theme	acids	803:807	arg1	acids					803:807	short-chain fatty acids	785:807	short-chain fatty acids (SCFAs)	785:815	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	5	33	theme	acids	803:807	arg1	variety					774:780	a variety	772:780	a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures	772:861	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
34662794	6	34	theme	flora	919:923	arg1	composition					893:903	the composition	889:903	the composition of intestinal flora	889:923	Meanwhile, PEP regulated the composition of intestinal flora, and the relative abundance of Firmicutes increased significantly.					
34662794	0	35	dep	Pleurotus	40:48	arg1	eryngii					50:56	Pleurotus eryngii	40:56	Pleurotus eryngii during simulated gastrointestinal digestion and fermentation	40:117	Characterization of polysaccharide from Pleurotus eryngii during simulated gastrointestinal digestion and fermentation.					
34662794	1	36	theme	valuable	143:150	arg1	variety					172:178	a valuable new edible mushroom variety	141:178	a valuable new edible mushroom variety cultivated on a large scale in China	141:215	Pleurotus eryngii is a valuable new edible mushroom variety cultivated on a large scale in China.					
34662794	1	36	theme	valuable	143:150	arg1	eryngii					130:136	Pleurotus eryngii	120:136	Pleurotus eryngii	120:136	Pleurotus eryngii is a valuable new edible mushroom variety cultivated on a large scale in China.					
34662794	6	37	theme	intestinal	908:917	arg1	flora					919:923	intestinal flora	908:923	intestinal flora	908:923	Meanwhile, PEP regulated the composition of intestinal flora, and the relative abundance of Firmicutes increased significantly.					
34662794	1	38	theme	new	152:154	arg1	mushroom					163:170	new edible mushroom	152:170	a valuable new edible mushroom variety cultivated on a large scale in China	141:215	Pleurotus eryngii is a valuable new edible mushroom variety cultivated on a large scale in China.					
34662794	1	39	from	scale	202:206	arg1	China					211:215	China	211:215	China	211:215	Pleurotus eryngii is a valuable new edible mushroom variety cultivated on a large scale in China.					
34662794	1	40	theme	edible	156:161	arg1	mushroom					163:170	new edible mushroom	152:170	a valuable new edible mushroom variety cultivated on a large scale in China	141:215	Pleurotus eryngii is a valuable new edible mushroom variety cultivated on a large scale in China.					
34662794	1	41	theme	mushroom	163:170	arg1	variety					172:178	a valuable new edible mushroom variety	141:178	a valuable new edible mushroom variety cultivated on a large scale in China	141:215	Pleurotus eryngii is a valuable new edible mushroom variety cultivated on a large scale in China.					
34662794	1	41	theme	mushroom	163:170	arg1	eryngii					130:136	Pleurotus eryngii	120:136	Pleurotus eryngii	120:136	Pleurotus eryngii is a valuable new edible mushroom variety cultivated on a large scale in China.					
34662794	3	42	theme	digestion	355:363	arg1	characteristics					382:396	the digestion and fermentation characteristics	351:396	characteristics	382:396	This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota.					
34662794	4	43	theme	PEP	522:524	arg1	weight					512:517	the molecular weight	498:517	the molecular weight of PEP	498:524	The results showed that the molecular weight of PEP remained unchanged after simulated digestion, and the overall structure of PEP was not destroyed, indicating that PEP was not decomposed during digestion.					
34662794	0	44	theme	polysaccharide	20:33	arg1	Characterization					0:15	Characterization	0:15	Characterization of polysaccharide from Pleurotus eryngii during simulated gastrointestinal digestion and fermentation.	0:118	Characterization of polysaccharide from Pleurotus eryngii during simulated gastrointestinal digestion and fermentation.					
34662794	5	45	from	value	839:843	arg1	cultures					854:861	fecal cultures	848:861	fecal cultures	848:861	However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures.					
33639188	0	0	theme	Cordyceps	78:86	arg1	mycelia					98:104	Cordyceps militaris mycelia	78:104	Cordyceps militaris mycelia	78:104	Structure analysis and antioxidant activity of polysaccharide-iron (III) from Cordyceps militaris mycelia.					
33639188	6	1	theme	iron	570:573	arg1	%					583:583	2.73%	579:583	2.73%	579:583	The content of iron was 2.73%.					
33639188	6	1	theme	iron	570:573	arg1	content					559:565	The content	555:565	The content of iron	555:573	The content of iron was 2.73%.					
33639188	1	2	theme	FeSO4	164:168	arg1	solution					170:177	FeSO4 solution	164:177	FeSO4 solution to the mycelia for biotransformation	164:214	Iron-enriched Cordyceps militaris was obtained by adding FeSO4 solution to the mycelia for biotransformation.					
33639188	7	3	theme	percentage	746:755	arg1	ratio					757:761	percentage ratio	746:761	percentage ratio of 0.94:3.12:27.01:36.62:30.20:2.12	746:797	The monosaccharide composition analysis indicated that the CPS-iron-II was composed of rhamnose, arabinose, galactose, glucose, mannose, galacturonic acid with percentage ratio of 0.94:3.12:27.01:36.62:30.20:2.12.					
33639188	10	4	theme	CPS-iron-II	1087:1097	arg1	activity					1075:1082	antioxidant activity	1063:1082	antioxidant activity of CPS-iron-II	1063:1097	The anti-oxidative analysis showed that antioxidant activity of CPS-iron-II was almost equal to that of Vc.					
33639188	4	5	theme	second	421:426	arg1	component					428:436	The second component	417:436	The second component	417:436	The second component was purified by Sephadex G-150 and named as CPS-iron-II.					
33639188	4	5	theme	second	421:426	arg1	CPS-iron-II					482:492	CPS-iron-II	482:492	CPS-iron-II	482:492	The second component was purified by Sephadex G-150 and named as CPS-iron-II.					
33639188	9	6	theme	Congo-red	938:946	arg1	test					948:951	Congo-red test	938:951	Congo-red test	938:951	Congo-red test showed that CPS-iron-II can cause flocculation of Congo red solution.					
33639188	8	7	dep	-α-D-Glcp-	907:916	arg1	4					905:905	4	905:905	4	905:905	The results of methylation analysis revealed that the CPS-iron-II was made of →2)-β-D-Glcp-(1→, with →2, 4)-α-D-Glcp-(1→ highly branched.					
33639188	11	8	from	militaris	1228:1236	arg1	preparation					1173:1183	the preparation	1169:1183	the preparation of polysaccharide-iron(III) from Cordyceps militaris	1169:1236	The manuscript provided a new way for the preparation of polysaccharide-iron(III) from Cordyceps militaris.					
33639188	0	9	theme	militaris	88:96	arg1	mycelia					98:104	Cordyceps militaris mycelia	78:104	Cordyceps militaris mycelia	78:104	Structure analysis and antioxidant activity of polysaccharide-iron (III) from Cordyceps militaris mycelia.					
33639188	2	10	theme	alcohol	285:291	arg1	precipitation					293:305	alcohol precipitation	285:305	alcohol precipitation	285:305	The polysaccharide-iron (III) was extracted by water extraction and alcohol precipitation.					
33639188	9	11	theme	solution	1013:1020	arg1	flocculation					987:998	flocculation	987:998	flocculation of Congo red solution	987:1020	Congo-red test showed that CPS-iron-II can cause flocculation of Congo red solution.					
33639188	5	12	theme	CPS-iron-II	527:537	arg1	44.136 kDa					543:552	44.136 kDa	543:552	44.136 kDa	543:552	The average molecular weight of CPS-iron-II was 44.136 kDa.					
33639188	5	12	theme	CPS-iron-II	527:537	arg1	weight					517:522	The average molecular weight	495:522	The average molecular weight of CPS-iron-II	495:537	The average molecular weight of CPS-iron-II was 44.136 kDa.					
33639188	3	13	contain	had	395:397	arg1	III					390:392	III	390:392	III	390:392	High performance liquid chromatography showed that the crude polysaccharide-iron (III) had three components.					
33639188	3	13	contain	had	395:397	arg1	polysaccharide-iron					369:387	the crude polysaccharide-iron	359:387	the crude polysaccharide-iron (III)	359:393	High performance liquid chromatography showed that the crude polysaccharide-iron (III) had three components.					
33639188	3	13	contain	had	395:397	arg2	components					405:414	three components	399:414	three components	399:414	High performance liquid chromatography showed that the crude polysaccharide-iron (III) had three components.					
33639188	7	14	with	glucose	705:711	arg1	ratio					757:761	percentage ratio	746:761	percentage ratio of 0.94:3.12:27.01:36.62:30.20:2.12	746:797	The monosaccharide composition analysis indicated that the CPS-iron-II was composed of rhamnose, arabinose, galactose, glucose, mannose, galacturonic acid with percentage ratio of 0.94:3.12:27.01:36.62:30.20:2.12.					
33639188	0	15	theme	Structure	0:8	arg1	analysis					10:17	Structure analysis	0:17	Structure analysis	0:17	Structure analysis and antioxidant activity of polysaccharide-iron (III) from Cordyceps militaris mycelia.					
33639188	7	16	theme	monosaccharide	590:603	arg1	analysis					617:624	The monosaccharide composition analysis	586:624	The monosaccharide composition analysis	586:624	The monosaccharide composition analysis indicated that the CPS-iron-II was composed of rhamnose, arabinose, galactose, glucose, mannose, galacturonic acid with percentage ratio of 0.94:3.12:27.01:36.62:30.20:2.12.					
33639188	10	17	theme	anti-oxidative	1027:1040	arg1	analysis					1042:1049	The anti-oxidative analysis	1023:1049	The anti-oxidative analysis	1023:1049	The anti-oxidative analysis showed that antioxidant activity of CPS-iron-II was almost equal to that of Vc.					
33639188	0	18	theme	antioxidant	23:33	arg1	activity					35:42	antioxidant activity	23:42	antioxidant activity	23:42	Structure analysis and antioxidant activity of polysaccharide-iron (III) from Cordyceps militaris mycelia.					
33639188	11	19	theme	polysaccharide-iron	1188:1206	arg1	preparation					1173:1183	the preparation	1169:1183	the preparation of polysaccharide-iron(III) from Cordyceps militaris	1169:1236	The manuscript provided a new way for the preparation of polysaccharide-iron(III) from Cordyceps militaris.					
33639188	7	20	theme	composition	605:615	arg1	analysis					617:624	The monosaccharide composition analysis	586:624	The monosaccharide composition analysis	586:624	The monosaccharide composition analysis indicated that the CPS-iron-II was composed of rhamnose, arabinose, galactose, glucose, mannose, galacturonic acid with percentage ratio of 0.94:3.12:27.01:36.62:30.20:2.12.					
33639188	1	21	theme	Iron-enriched	107:119	arg1	militaris					131:139	Iron-enriched Cordyceps militaris	107:139	Iron-enriched Cordyceps militaris	107:139	Iron-enriched Cordyceps militaris was obtained by adding FeSO4 solution to the mycelia for biotransformation.					
33639188	5	22	theme	average	499:505	arg1	44.136 kDa					543:552	44.136 kDa	543:552	44.136 kDa	543:552	The average molecular weight of CPS-iron-II was 44.136 kDa.					
33639188	5	22	theme	average	499:505	arg1	weight					517:522	The average molecular weight	495:522	The average molecular weight of CPS-iron-II	495:537	The average molecular weight of CPS-iron-II was 44.136 kDa.					
33639188	7	23	theme	galacturonic	723:734	arg1	acid					736:739	galacturonic acid	723:739	galacturonic acid	723:739	The monosaccharide composition analysis indicated that the CPS-iron-II was composed of rhamnose, arabinose, galactose, glucose, mannose, galacturonic acid with percentage ratio of 0.94:3.12:27.01:36.62:30.20:2.12.					
33639188	7	23	theme	galacturonic	723:734	arg1	glucose					705:711	glucose	705:711	glucose	705:711	The monosaccharide composition analysis indicated that the CPS-iron-II was composed of rhamnose, arabinose, galactose, glucose, mannose, galacturonic acid with percentage ratio of 0.94:3.12:27.01:36.62:30.20:2.12.					
33639188	5	24	theme	molecular	507:515	arg1	44.136 kDa					543:552	44.136 kDa	543:552	44.136 kDa	543:552	The average molecular weight of CPS-iron-II was 44.136 kDa.					
33639188	5	24	theme	molecular	507:515	arg1	weight					517:522	The average molecular weight	495:522	The average molecular weight of CPS-iron-II	495:537	The average molecular weight of CPS-iron-II was 44.136 kDa.					
33639188	11	25	theme	Cordyceps	1218:1226	arg1	militaris					1228:1236	Cordyceps militaris	1218:1236	Cordyceps militaris	1218:1236	The manuscript provided a new way for the preparation of polysaccharide-iron(III) from Cordyceps militaris.					
33639188	9	26	theme	red	1009:1011	arg1	solution					1013:1020	Congo red solution	1003:1020	Congo red solution	1003:1020	Congo-red test showed that CPS-iron-II can cause flocculation of Congo red solution.					
33639188	3	27	theme	liquid	325:330	arg1	chromatography					332:345	High performance liquid chromatography	308:345	High performance liquid chromatography	308:345	High performance liquid chromatography showed that the crude polysaccharide-iron (III) had three components.					
33639188	1	28	theme	Cordyceps	121:129	arg1	militaris					131:139	Iron-enriched Cordyceps militaris	107:139	Iron-enriched Cordyceps militaris	107:139	Iron-enriched Cordyceps militaris was obtained by adding FeSO4 solution to the mycelia for biotransformation.					
33639188	0	29	theme	polysaccharide-iron	47:65	arg1	analysis					10:17	Structure analysis	0:17	Structure analysis	0:17	Structure analysis and antioxidant activity of polysaccharide-iron (III) from Cordyceps militaris mycelia.					
33639188	0	29	theme	polysaccharide-iron	47:65	arg1	activity					35:42	antioxidant activity	23:42	antioxidant activity	23:42	Structure analysis and antioxidant activity of polysaccharide-iron (III) from Cordyceps militaris mycelia.					
33639188	3	30	theme	High	308:311	arg1	chromatography					332:345	High performance liquid chromatography	308:345	High performance liquid chromatography	308:345	High performance liquid chromatography showed that the crude polysaccharide-iron (III) had three components.					
33639188	10	31	theme	antioxidant	1063:1073	arg1	activity					1075:1082	antioxidant activity	1063:1082	antioxidant activity of CPS-iron-II	1063:1097	The anti-oxidative analysis showed that antioxidant activity of CPS-iron-II was almost equal to that of Vc.					
33639188	11	32	theme	new	1157:1159	arg1	way					1161:1163	a new way	1155:1163	a new way for the preparation of polysaccharide-iron(III) from Cordyceps militaris	1155:1236	The manuscript provided a new way for the preparation of polysaccharide-iron(III) from Cordyceps militaris.					
33639188	8	33	theme	branched	928:935	arg1	→2					901:902	→2	901:902	→2	901:902	The results of methylation analysis revealed that the CPS-iron-II was made of →2)-β-D-Glcp-(1→, with →2, 4)-α-D-Glcp-(1→ highly branched.					
33639188	8	33	theme	branched	928:935	arg1	-α-D-Glcp-					907:916	4)-α-D-Glcp-(1→ highly branched	905:935	4)-α-D-Glcp-(1→ highly branched	905:935	The results of methylation analysis revealed that the CPS-iron-II was made of →2)-β-D-Glcp-(1→, with →2, 4)-α-D-Glcp-(1→ highly branched.					
33639188	3	34	theme	performance	313:323	arg1	chromatography					332:345	High performance liquid chromatography	308:345	High performance liquid chromatography	308:345	High performance liquid chromatography showed that the crude polysaccharide-iron (III) had three components.					
33639188	0	35	from	mycelia	98:104	arg1	analysis					10:17	Structure analysis	0:17	Structure analysis	0:17	Structure analysis and antioxidant activity of polysaccharide-iron (III) from Cordyceps militaris mycelia.					
33639188	0	35	from	mycelia	98:104	arg1	activity					35:42	antioxidant activity	23:42	antioxidant activity	23:42	Structure analysis and antioxidant activity of polysaccharide-iron (III) from Cordyceps militaris mycelia.					
33639188	2	36	theme	water	264:268	arg1	extraction					270:279	water extraction	264:279	water extraction	264:279	The polysaccharide-iron (III) was extracted by water extraction and alcohol precipitation.					
33639188	4	37	theme	Sephadex	454:461	arg1	G-150					463:467	Sephadex G-150	454:467	Sephadex G-150	454:467	The second component was purified by Sephadex G-150 and named as CPS-iron-II.					
33639188	8	38	theme	analysis	827:834	arg1	results					804:810	The results	800:810	The results of methylation analysis	800:834	The results of methylation analysis revealed that the CPS-iron-II was made of →2)-β-D-Glcp-(1→, with →2, 4)-α-D-Glcp-(1→ highly branched.					
33639188	3	39	theme	crude	363:367	arg1	III					390:392	III	390:392	III	390:392	High performance liquid chromatography showed that the crude polysaccharide-iron (III) had three components.					
33639188	3	39	theme	crude	363:367	arg1	polysaccharide-iron					369:387	the crude polysaccharide-iron	359:387	the crude polysaccharide-iron (III)	359:393	High performance liquid chromatography showed that the crude polysaccharide-iron (III) had three components.					
33639188	8	40	theme	methylation	815:825	arg1	analysis					827:834	methylation analysis	815:834	methylation analysis	815:834	The results of methylation analysis revealed that the CPS-iron-II was made of →2)-β-D-Glcp-(1→, with →2, 4)-α-D-Glcp-(1→ highly branched.					
33639188	8	41	theme	-β-D-Glcp-	881:890	arg1	1→					892:893	→2)-β-D-Glcp-(1→	878:893	→2)-β-D-Glcp-(1→	878:893	The results of methylation analysis revealed that the CPS-iron-II was made of →2)-β-D-Glcp-(1→, with →2, 4)-α-D-Glcp-(1→ highly branched.					
33639188	7	42	theme	0.94:3.12:27.01:36.62:30.20:2.12	766:797	arg1	ratio					757:761	percentage ratio	746:761	percentage ratio of 0.94:3.12:27.01:36.62:30.20:2.12	746:797	The monosaccharide composition analysis indicated that the CPS-iron-II was composed of rhamnose, arabinose, galactose, glucose, mannose, galacturonic acid with percentage ratio of 0.94:3.12:27.01:36.62:30.20:2.12.					
32417546	0	0	theme	chitin	108:113	arg1	cross-linking					91:103	cross-linking	91:103	cross-linking of chitin	91:113	Chemoenzymatic synthesis of carboxylate-terminated maltooligosaccharides and their use for cross-linking of chitin.					
32417546	1	1	theme	maltooligosaccharides	169:189	arg1	synthesis					156:164	chemoenzymatic synthesis	141:164	chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa)	141:295	In this paper, we report chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa).					
32417546	5	2	theme	condensing	895:904	arg1	agent					906:910	condensing agent	895:910	condensing agent	895:910	The obtained GlcA-Glcn-GlcCOONa were used as cross-linker for WSCh by condensation in the presence of condensing agent.					
32417546	7	3	theme	lyophilized	1000:1010	arg1	cryogels					1021:1028	cryogels	1021:1028	cryogels	1021:1028	Morphologies of lyophilized samples (cryogels) from the hydrogels were evaluated by SEM measurement.					
32417546	7	3	theme	lyophilized	1000:1010	arg1	samples					1012:1018	lyophilized samples	1000:1018	lyophilized samples (cryogels) from the hydrogels	1000:1048	Morphologies of lyophilized samples (cryogels) from the hydrogels were evaluated by SEM measurement.					
32417546	3	4	theme	reducing	649:656	arg1	end					658:660	the reducing end	645:660	the reducing end of maltoheptaose with sodium hypoiodite	645:700	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	3	5	theme	maltoheptaose	665:677	arg1	end					658:660	the reducing end	645:660	the reducing end of maltoheptaose with sodium hypoiodite	645:700	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	1	6	contain	having	191:196	arg1	maltooligosaccharides					169:189	maltooligosaccharides	169:189	maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa)	169:295	In this paper, we report chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa).					
32417546	1	6	contain	having	191:196	arg2	groups					210:215	carboxylate groups	198:215	carboxylate groups	198:215	In this paper, we report chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa).					
32417546	9	7	theme	films	1198:1202	arg1	properties					1166:1175	mechanical properties	1155:1175	mechanical properties of the hydrogels and films	1155:1202	Furthermore, mechanical properties of the hydrogels and films were investigated by compression and tensile tests, respectively.					
32417546	7	8	from	hydrogels	1040:1048	arg1	cryogels					1021:1028	cryogels	1021:1028	cryogels	1021:1028	Morphologies of lyophilized samples (cryogels) from the hydrogels were evaluated by SEM measurement.					
32417546	7	8	from	hydrogels	1040:1048	arg1	Morphologies					984:995	Morphologies	984:995	Morphologies of lyophilized samples (cryogels) from the hydrogels	984:1048	Morphologies of lyophilized samples (cryogels) from the hydrogels were evaluated by SEM measurement.					
32417546	7	8	from	hydrogels	1040:1048	arg1	samples					1012:1018	lyophilized samples	1000:1018	lyophilized samples (cryogels) from the hydrogels	1000:1048	Morphologies of lyophilized samples (cryogels) from the hydrogels were evaluated by SEM measurement.					
32417546	9	9	theme	mechanical	1155:1164	arg1	properties					1166:1175	mechanical properties	1155:1175	mechanical properties of the hydrogels and films	1155:1202	Furthermore, mechanical properties of the hydrogels and films were investigated by compression and tensile tests, respectively.					
32417546	1	10	theme	carboxylate	198:208	arg1	groups					210:215	carboxylate groups	198:215	carboxylate groups	198:215	In this paper, we report chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa).					
32417546	5	11	used	used	830:833	arg2	cross-linker					838:849	cross-linker	838:849	cross-linker	838:849	The obtained GlcA-Glcn-GlcCOONa were used as cross-linker for WSCh by condensation in the presence of condensing agent.					
32417546	5	11	used	used	830:833	arg2	GlcA-Glcn-GlcCOONa					806:823	The obtained GlcA-Glcn-GlcCOONa	793:823	The obtained GlcA-Glcn-GlcCOONa	793:823	The obtained GlcA-Glcn-GlcCOONa were used as cross-linker for WSCh by condensation in the presence of condensing agent.					
32417546	5	12	theme	agent	906:910	arg1	presence					883:890	the presence	879:890	the presence of condensing agent	879:910	The obtained GlcA-Glcn-GlcCOONa were used as cross-linker for WSCh by condensation in the presence of condensing agent.					
32417546	6	13	theme	reaction	917:924	arg1	mixtures					926:933	The reaction mixtures	913:933	The reaction mixtures	913:933	The reaction mixtures totally turned into hydrogel form in most cases.					
32417546	3	14	with	maltoheptaose	665:677	arg1	hypoiodite					691:700	sodium hypoiodite	684:700	sodium hypoiodite	684:700	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	3	15	theme	acid	542:545	arg1	1-phosphate					547:557	α-d-glucuronic acid 1-phosphate	527:557	α-d-glucuronic acid 1-phosphate	527:557	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	7	16	theme	SEM	1068:1070	arg1	measurement					1072:1082	SEM measurement	1068:1082	SEM measurement	1068:1082	Morphologies of lyophilized samples (cryogels) from the hydrogels were evaluated by SEM measurement.					
32417546	0	17	theme	Chemoenzymatic	0:13	arg1	synthesis					15:23	Chemoenzymatic synthesis	0:23	Chemoenzymatic synthesis of carboxylate-terminated maltooligosaccharides	0:71	Chemoenzymatic synthesis of carboxylate-terminated maltooligosaccharides and their use for cross-linking of chitin.					
32417546	6	18	theme	hydrogel	955:962	arg1	form					964:967	hydrogel form	955:967	hydrogel form in most cases	955:981	The reaction mixtures totally turned into hydrogel form in most cases.					
32417546	4	19	theme	mass	779:782	arg1	spectra					784:790	MALDI-TOF mass spectra	769:790	MALDI-TOF mass spectra	769:790	The structures of GlcA-Glcn-GlcCOONa were evaluated by 1H NMR and MALDI-TOF mass spectra.					
32417546	3	20	theme	sodium	684:689	arg1	hypoiodite					691:700	sodium hypoiodite	684:700	sodium hypoiodite	684:700	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	0	21	theme	carboxylate-terminated	28:49	arg1	maltooligosaccharides					51:71	carboxylate-terminated maltooligosaccharides	28:71	carboxylate-terminated maltooligosaccharides	28:71	Chemoenzymatic synthesis of carboxylate-terminated maltooligosaccharides and their use for cross-linking of chitin.					
32417546	7	22	from	Morphologies	984:995	arg1	hydrogels					1040:1048	the hydrogels	1036:1048	the hydrogels	1036:1048	Morphologies of lyophilized samples (cryogels) from the hydrogels were evaluated by SEM measurement.					
32417546	4	23	theme	1H	758:759	arg1	NMR					761:763	1H NMR	758:763	1H NMR	758:763	The structures of GlcA-Glcn-GlcCOONa were evaluated by 1H NMR and MALDI-TOF mass spectra.					
32417546	3	24	theme	GlcA-Glcn-GlcCOONa	421:438	arg1	synthesis					408:416	The synthesis	404:416	The synthesis of GlcA-Glcn-GlcCOONa	404:438	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	2	25	theme	water-soluble	349:361	arg1	chitin					363:368	water-soluble chitin	349:368	water-soluble chitin (WSCh)	349:375	The products were further used as cross-linker for water-soluble chitin (WSCh) to obtain network chitins.					
32417546	2	25	theme	water-soluble	349:361	arg1	WSCh					371:374	WSCh	371:374	WSCh	371:374	The products were further used as cross-linker for water-soluble chitin (WSCh) to obtain network chitins.					
32417546	9	26	theme	tensile	1241:1247	arg1	tests					1249:1253	tensile tests	1241:1253	tensile tests	1241:1253	Furthermore, mechanical properties of the hydrogels and films were investigated by compression and tensile tests, respectively.					
32417546	3	27	theme	carboxylated	566:577	arg1	maltooligosaccharide					579:598	a carboxylated maltooligosaccharide	564:598	a carboxylated maltooligosaccharide	564:598	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	3	28	theme	phosphorylase-catalyzed	469:491	arg1	α-glucuronylation					503:519	thermostable phosphorylase-catalyzed enzymatic α-glucuronylation	456:519	thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite	456:700	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	9	29	theme	hydrogels	1184:1192	arg1	properties					1166:1175	mechanical properties	1155:1175	mechanical properties of the hydrogels and films	1155:1202	Furthermore, mechanical properties of the hydrogels and films were investigated by compression and tensile tests, respectively.					
32417546	1	30	theme	carboxylate-terminated	231:252	arg1	GlcA-Glcn-GlcCOONa					277:294	GlcA-Glcn-GlcCOONa	277:294	GlcA-Glcn-GlcCOONa	277:294	In this paper, we report chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa).					
32417546	1	30	theme	carboxylate-terminated	231:252	arg1	maltooligosaccharides					254:274	carboxylate-terminated maltooligosaccharides	231:274	carboxylate-terminated maltooligosaccharides	231:274	In this paper, we report chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa).					
32417546	0	31	theme	maltooligosaccharides	51:71	arg1	synthesis					15:23	Chemoenzymatic synthesis	0:23	Chemoenzymatic synthesis of carboxylate-terminated maltooligosaccharides	0:71	Chemoenzymatic synthesis of carboxylate-terminated maltooligosaccharides and their use for cross-linking of chitin.					
32417546	0	31	theme	maltooligosaccharides	51:71	arg1	use					83:85	their use	77:85	their use for cross-linking of chitin	77:113	Chemoenzymatic synthesis of carboxylate-terminated maltooligosaccharides and their use for cross-linking of chitin.					
32417546	3	32	theme	enzymatic	493:501	arg1	α-glucuronylation					503:519	thermostable phosphorylase-catalyzed enzymatic α-glucuronylation	456:519	thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite	456:700	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	5	33	from	WSCh	855:858	arg1	presence					883:890	the presence	879:890	the presence of condensing agent	879:910	The obtained GlcA-Glcn-GlcCOONa were used as cross-linker for WSCh by condensation in the presence of condensing agent.					
32417546	3	34	theme	α-d-glucuronic	527:540	arg1	1-phosphate					547:557	α-d-glucuronic acid 1-phosphate	527:557	α-d-glucuronic acid 1-phosphate	527:557	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	2	35	theme	network	387:393	arg1	chitins					395:401	network chitins	387:401	network chitins	387:401	The products were further used as cross-linker for water-soluble chitin (WSCh) to obtain network chitins.					
32417546	3	36	theme	thermostable	456:467	arg1	α-glucuronylation					503:519	thermostable phosphorylase-catalyzed enzymatic α-glucuronylation	456:519	thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite	456:700	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	5	37	theme	obtained	797:804	arg1	GlcA-Glcn-GlcCOONa					806:823	The obtained GlcA-Glcn-GlcCOONa	793:823	The obtained GlcA-Glcn-GlcCOONa	793:823	The obtained GlcA-Glcn-GlcCOONa were used as cross-linker for WSCh by condensation in the presence of condensing agent.					
32417546	5	37	theme	obtained	797:804	arg1	cross-linker					838:849	cross-linker	838:849	cross-linker	838:849	The obtained GlcA-Glcn-GlcCOONa were used as cross-linker for WSCh by condensation in the presence of condensing agent.					
32417546	6	38	theme	most	972:975	arg1	cases					977:981	most cases	972:981	most cases	972:981	The reaction mixtures totally turned into hydrogel form in most cases.					
32417546	4	39	theme	GlcA-Glcn-GlcCOONa	721:738	arg1	structures					707:716	The structures	703:716	The structures of GlcA-Glcn-GlcCOONa	703:738	The structures of GlcA-Glcn-GlcCOONa were evaluated by 1H NMR and MALDI-TOF mass spectra.					
32417546	1	40	theme	chemoenzymatic	141:154	arg1	synthesis					156:164	chemoenzymatic synthesis	141:164	chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa)	141:295	In this paper, we report chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa).					
32417546	7	41	theme	samples	1012:1018	arg1	Morphologies					984:995	Morphologies	984:995	Morphologies of lyophilized samples (cryogels) from the hydrogels	984:1048	Morphologies of lyophilized samples (cryogels) from the hydrogels were evaluated by SEM measurement.					
32417546	3	42	from	end	658:660	arg1	oxidation					632:640	chemical oxidation	623:640	chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite	623:700	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	1	43	dep	ends	225:228	arg1	GlcA-Glcn-GlcCOONa					277:294	GlcA-Glcn-GlcCOONa	277:294	GlcA-Glcn-GlcCOONa	277:294	In this paper, we report chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa).					
32417546	1	43	dep	ends	225:228	arg1	maltooligosaccharides					254:274	carboxylate-terminated maltooligosaccharides	231:274	carboxylate-terminated maltooligosaccharides	231:274	In this paper, we report chemoenzymatic synthesis of maltooligosaccharides having carboxylate groups at both ends (carboxylate-terminated maltooligosaccharides, GlcA-Glcn-GlcCOONa).					
32417546	3	44	theme	chemical	623:630	arg1	oxidation					632:640	chemical oxidation	623:640	chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite	623:700	The synthesis of GlcA-Glcn-GlcCOONa was achieved by thermostable phosphorylase-catalyzed enzymatic α-glucuronylation using α-d-glucuronic acid 1-phosphate with a carboxylated maltooligosaccharide, which was prepared by chemical oxidation at the reducing end of maltoheptaose with sodium hypoiodite.					
32417546	4	45	theme	MALDI-TOF	769:777	arg1	spectra					784:790	MALDI-TOF mass spectra	769:790	MALDI-TOF mass spectra	769:790	The structures of GlcA-Glcn-GlcCOONa were evaluated by 1H NMR and MALDI-TOF mass spectra.					
32417546	6	46	from	form	964:967	arg1	cases					977:981	most cases	972:981	most cases	972:981	The reaction mixtures totally turned into hydrogel form in most cases.					
32417546	2	47	used	used	324:327	arg2	products					302:309	The products	298:309	The products	298:309	The products were further used as cross-linker for water-soluble chitin (WSCh) to obtain network chitins.					
32417546	2	47	used	used	324:327	arg2	cross-linker					332:343	cross-linker	332:343	cross-linker for water-soluble chitin (WSCh)	332:375	The products were further used as cross-linker for water-soluble chitin (WSCh) to obtain network chitins.					
32936008	7	0	theme	wheat	1443:1447	arg1	starch					1449:1454	wheat starch	1443:1454	wheat starch	1443:1454	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	2	1	theme	functional	544:553	arg1	profile					555:561	predictive functional profile	533:561	predictive functional profile	533:561	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	1	2	theme	dynamic	240:246	arg1	model					270:274	no dynamic in vitro fermentation model	237:274	no dynamic in vitro fermentation model for pigs	237:283	Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs.					
32936008	4	3	theme	network	828:834	arg1	properties					848:857	the network topological properties	824:857	the network topological properties	824:857	Co-occurrence network analysis indicated that microbiota fed with different sources of starch changed the network topological properties.					
32936008	7	4	theme	maize	1372:1376	arg1	starch					1378:1383	maize starch	1372:1383	maize starch	1372:1383	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	5	5	theme	Functional	860:869	arg1	profiles					871:878	Functional profiles	860:878	Functional profiles	860:878	Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment.					
32936008	2	6	theme	short-chain	568:578	arg1	acids					586:590	short-chain fatty acids	568:590	short-chain fatty acids production	568:601	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	7	7	theme	acid	1364:1367	arg1	production					1319:1328	The cumulative production	1304:1328	The cumulative production of acetic, propionic, and butyric acid on maize starch	1304:1383	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	7	7	theme	acid	1364:1367	arg1	higher					1404:1409	higher	1404:1409	higher	1404:1409	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	8	8	theme	wheat	1784:1788	arg1	inoculum					1725:1732	original inoculum	1716:1732	original inoculum	1716:1732	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	8	8	theme	wheat	1784:1788	arg1	starch					1790:1795	wheat starch	1784:1795	wheat starch	1784:1795	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	9	9	theme	basal	1813:1817	arg1	diet					1819:1822	A maize basal diet	1805:1822	A maize basal diet	1805:1822	A maize basal diet may improve energy absorption in the large intestine in growing pigs.					
32936008	8	10	from	model	1692:1696	arg1	stable					1669:1674	stable	1669:1674	stable	1669:1674	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	8	10	from	model	1692:1696	arg1	bacteria					1649:1656	the bacteria	1645:1656	the bacteria	1645:1656	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	3	11	theme	microbiota	698:707	arg1	composition					709:719	microbiota composition	698:719	microbiota composition	698:719	Our study showed that three different types of starch or two polysaccharides greatly impacted microbiota composition.					
32936008	0	12	theme	porcine	113:119	arg1	microbiota					125:134	a porcine gut microbiota	111:134	a porcine gut microbiota	111:134	Polysaccharide source altered ecological network, functional profile, and short-chain fatty acid production in a porcine gut microbiota.					
32936008	2	13	theme	swine	485:489	arg1	structure					502:510	swine microbiota structure	485:510	swine microbiota structure	485:510	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	7	14	theme	propionic	1341:1349	arg1	acid					1364:1367	acetic, propionic, and butyric acid	1333:1367	acetic, propionic, and butyric acid	1333:1367	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	7	15	theme	acetic	1333:1338	arg1	acid					1364:1367	acetic, propionic, and butyric acid	1333:1367	acetic, propionic, and butyric acid	1333:1367	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	9	16	theme	energy	1836:1841	arg1	absorption					1843:1852	energy absorption	1836:1852	energy absorption	1836:1852	A maize basal diet may improve energy absorption in the large intestine in growing pigs.					
32936008	6	17	theme	inoculum	1268:1275	arg1	profile					1244:1250	the functional profile	1229:1250	the functional profile of the original inoculum	1229:1275	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	6	18	theme	functional	1233:1242	arg1	profile					1244:1250	the functional profile	1229:1250	the functional profile of the original inoculum	1229:1275	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	2	19	theme	ecological	513:522	arg1	network					524:530	ecological network	513:530	ecological network	513:530	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	7	20	dep	significant	1498:1508	arg1	significant					1498:1508	significant	1498:1508	significant	1498:1508	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	7	20	dep	significant	1498:1508	arg1	amount					1472:1477	only the amount	1463:1477	only the amount of acetic acid	1463:1492	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	7	20	dep	significant	1498:1508	arg1	acid					1489:1492	acetic acid	1482:1492	acetic acid	1482:1492	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	7	20	dep	significant	1498:1508	arg1	higher					1510:1515	higher	1510:1515	higher	1510:1515	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	8	21	theme	in	1683:1684	arg1	model					1692:1696	the in vitro model	1679:1696	the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX	1679:1802	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	5	22	theme	starch	933:938	arg1	treatments					940:949	the three starch treatments	923:949	the three starch treatments	923:949	Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment.					
32936008	2	23	theme	different	437:445	arg1	sources					454:460	different starch sources	437:460	different starch sources	437:460	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	9	24	theme	large	1861:1865	arg1	intestine					1867:1875	the large intestine	1857:1875	the large intestine in growing pigs	1857:1891	A maize basal diet may improve energy absorption in the large intestine in growing pigs.					
32936008	1	25	theme	validated	145:153	arg1	models					172:177	Several validated dynamic in vitro models	137:177	Several validated dynamic in vitro models of the colon	137:190	Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs.					
32936008	0	26	theme	short-chain	74:84	arg1	acid					92:95	short-chain fatty acid	74:95	short-chain fatty acid production	74:106	Polysaccharide source altered ecological network, functional profile, and short-chain fatty acid production in a porcine gut microbiota.					
32936008	8	27	theme	closer	1702:1707	arg1	model					1692:1696	the in vitro model	1679:1696	the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX	1679:1802	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	1	28	theme	in	163:164	arg1	models					172:177	Several validated dynamic in vitro models	137:177	Several validated dynamic in vitro models of the colon	137:190	Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs.					
32936008	2	29	theme	polysaccharides	466:480	arg1	effects					426:432	effects	426:432	effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production	426:601	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	0	30	theme	acid	92:95	arg1	production					97:106	short-chain fatty acid production	74:106	short-chain fatty acid production	74:106	Polysaccharide source altered ecological network, functional profile, and short-chain fatty acid production in a porcine gut microbiota.					
32936008	5	31	theme	pig	969:971	arg1	similar					998:1004	similar	998:1004	similar	998:1004	Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment.					
32936008	5	31	theme	pig	969:971	arg1	inoculum					980:987	the original pig faecal inoculum	956:987	the original pig faecal inoculum	956:987	Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment.					
32936008	2	32	theme	sources	454:460	arg1	effects					426:432	effects	426:432	effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production	426:601	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	7	33	from	production	1319:1328	arg1	starch					1378:1383	maize starch	1372:1383	maize starch	1372:1383	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	0	34	theme	Polysaccharide	0:13	arg1	source					15:20	Polysaccharide source	0:20	Polysaccharide source	0:20	Polysaccharide source altered ecological network, functional profile, and short-chain fatty acid production in a porcine gut microbiota.					
32936008	1	35	theme	in	248:249	arg1	model					270:274	no dynamic in vitro fermentation model	237:274	no dynamic in vitro fermentation model for pigs	237:283	Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs.					
32936008	8	36	theme	microbial	1738:1746	arg1	function					1748:1755	microbial function	1738:1755	microbial function	1738:1755	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	2	37	theme	acids	586:590	arg1	production					592:601	short-chain fatty acids production	568:601	short-chain fatty acids production	568:601	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	2	38	theme	colon	387:391	arg1	model					374:378	the human, dynamic, computer-controlled TNO in vitro model	321:378	the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs	321:408	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	4	39	theme	Co-occurrence	722:734	arg1	analysis					744:751	Co-occurrence network analysis	722:751	Co-occurrence network analysis	722:751	Co-occurrence network analysis indicated that microbiota fed with different sources of starch changed the network topological properties.					
32936008	1	40	theme	fermentation	257:268	arg1	model					270:274	no dynamic in vitro fermentation model	237:274	no dynamic in vitro fermentation model for pigs	237:283	Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs.					
32936008	6	41	theme	inoculum	1145:1152	arg1	similar					1163:1169	similar	1163:1169	similar	1163:1169	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	6	41	theme	inoculum	1145:1152	arg1	composition					1117:1127	the microbial composition	1103:1127	the microbial composition of the original inoculum	1103:1152	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	6	42	theme	other	1040:1044	arg1	hand					1046:1049	the other hand	1036:1049	the other hand	1036:1049	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	5	43	theme	starch	1015:1020	arg1	treatment					1022:1030	maize starch treatment	1009:1030	maize starch treatment	1009:1030	Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment.					
32936008	0	44	theme	functional	50:59	arg1	profile					61:67	functional profile	50:67	functional profile	50:67	Polysaccharide source altered ecological network, functional profile, and short-chain fatty acid production in a porcine gut microbiota.					
32936008	3	45	theme	different	632:640	arg1	types					642:646	three different types	626:646	three different types of starch or two polysaccharides	626:679	Our study showed that three different types of starch or two polysaccharides greatly impacted microbiota composition.					
32936008	8	46	theme	maize	1577:1581	arg1	supplementation					1558:1572	supplementation	1558:1572	supplementation of maize starch as the starch source together with AX and XG	1558:1633	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	8	47	dep	in	1683:1684	arg1	vitro					1686:1690	vitro	1686:1690	vitro	1686:1690	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	4	48	theme	different	788:796	arg1	sources					798:804	different sources	788:804	different sources of starch	788:814	Co-occurrence network analysis indicated that microbiota fed with different sources of starch changed the network topological properties.					
32936008	8	49	theme	starch	1597:1602	arg1	source					1604:1609	the starch source	1593:1609	the starch source	1593:1609	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	7	50	theme	potato	1425:1430	arg1	starch					1432:1437	potato starch	1425:1437	potato starch	1425:1437	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	8	51	theme	potato	1769:1774	arg1	starch					1776:1781	potato starch	1769:1781	potato starch	1769:1781	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	2	52	theme	predictive	533:542	arg1	profile					555:561	predictive functional profile	533:561	predictive functional profile	533:561	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	2	53	theme	fatty	580:584	arg1	acids					586:590	short-chain fatty acids	568:590	short-chain fatty acids production	568:601	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	6	54	theme	original	1136:1143	arg1	inoculum					1145:1152	the original inoculum	1132:1152	the original inoculum	1132:1152	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	8	55	dep	inoculum	1725:1732	arg1	the					1712:1714	the	1712:1714	the	1712:1714	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	8	56	from	stable	1669:1674	arg1	model					1692:1696	the in vitro model	1679:1696	the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX	1679:1802	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	3	57	theme	polysaccharides	665:679	arg1	types					642:646	three different types	626:646	three different types of starch or two polysaccharides	626:679	Our study showed that three different types of starch or two polysaccharides greatly impacted microbiota composition.					
32936008	4	58	theme	topological	836:846	arg1	properties					848:857	the network topological properties	824:857	the network topological properties	824:857	Co-occurrence network analysis indicated that microbiota fed with different sources of starch changed the network topological properties.					
32936008	9	59	theme	maize	1807:1811	arg1	diet					1819:1822	A maize basal diet	1805:1822	A maize basal diet	1805:1822	A maize basal diet may improve energy absorption in the large intestine in growing pigs.					
32936008	0	60	theme	gut	121:123	arg1	microbiota					125:134	a porcine gut microbiota	111:134	a porcine gut microbiota	111:134	Polysaccharide source altered ecological network, functional profile, and short-chain fatty acid production in a porcine gut microbiota.					
32936008	4	61	theme	starch	809:814	arg1	sources					798:804	different sources	788:804	different sources of starch	788:814	Co-occurrence network analysis indicated that microbiota fed with different sources of starch changed the network topological properties.					
32936008	1	62	theme	colon	186:190	arg1	models					172:177	Several validated dynamic in vitro models	137:177	Several validated dynamic in vitro models of the colon	137:190	Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs.					
32936008	2	63	theme	microbiota	491:500	arg1	structure					502:510	swine microbiota structure	485:510	swine microbiota structure	485:510	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	2	64	from	effects	426:432	arg1	profile					555:561	predictive functional profile	533:561	predictive functional profile	533:561	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	2	64	from	effects	426:432	arg1	production					592:601	short-chain fatty acids production	568:601	short-chain fatty acids production	568:601	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	2	64	from	effects	426:432	arg1	structure					502:510	swine microbiota structure	485:510	swine microbiota structure	485:510	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	2	64	from	effects	426:432	arg1	network					524:530	ecological network	513:530	ecological network	513:530	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	7	65	theme	butyric	1356:1362	arg1	acid					1364:1367	acetic, propionic, and butyric acid	1333:1367	acetic, propionic, and butyric acid	1333:1367	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	8	66	theme	AX	1801:1802	arg1	model					1692:1696	the in vitro model	1679:1696	the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX	1679:1802	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	6	67	theme	original	1259:1266	arg1	inoculum					1268:1275	the original inoculum	1255:1275	the original inoculum	1255:1275	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	6	68	dep	AX-XG	1176:1180	arg1	xyloglucan					1201:1210	xyloglucan	1201:1210	xyloglucan	1201:1210	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	6	68	dep	AX-XG	1176:1180	arg1	arabinoxylans					1183:1195	arabinoxylans	1183:1195	arabinoxylans	1183:1195	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	1	69	theme	Several	137:143	arg1	models					172:177	Several validated dynamic in vitro models	137:177	Several validated dynamic in vitro models of the colon	137:190	Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs.					
32936008	7	70	theme	cumulative	1308:1317	arg1	production					1319:1328	The cumulative production	1304:1328	The cumulative production of acetic, propionic, and butyric acid on maize starch	1304:1383	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	7	70	theme	cumulative	1308:1317	arg1	higher					1404:1409	higher	1404:1409	higher	1404:1409	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	0	71	theme	fatty	86:90	arg1	acid					92:95	short-chain fatty acid	74:95	short-chain fatty acid production	74:106	Polysaccharide source altered ecological network, functional profile, and short-chain fatty acid production in a porcine gut microbiota.					
32936008	1	72	theme	dynamic	155:161	arg1	models					172:177	Several validated dynamic in vitro models	137:177	Several validated dynamic in vitro models of the colon	137:190	Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs.					
32936008	1	73	dep	in	248:249	arg1	vitro					251:255	vitro	251:255	vitro	251:255	Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs.					
32936008	9	74	theme	growing	1880:1886	arg1	pigs					1888:1891	growing pigs	1880:1891	growing pigs	1880:1891	A maize basal diet may improve energy absorption in the large intestine in growing pigs.					
32936008	5	75	theme	original	960:967	arg1	similar					998:1004	similar	998:1004	similar	998:1004	Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment.					
32936008	5	75	theme	original	960:967	arg1	inoculum					980:987	the original pig faecal inoculum	956:987	the original pig faecal inoculum	956:987	Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment.					
32936008	6	76	theme	microbial	1107:1115	arg1	similar					1163:1169	similar	1163:1169	similar	1163:1169	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	6	76	theme	microbial	1107:1115	arg1	composition					1117:1127	the microbial composition	1103:1127	the microbial composition of the original inoculum	1103:1152	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	7	77	theme	acid	1489:1492	arg1	significant					1498:1508	significant	1498:1508	significant	1498:1508	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	7	77	theme	acid	1489:1492	arg1	amount					1472:1477	only the amount	1463:1477	only the amount of acetic acid	1463:1492	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	7	77	theme	acid	1489:1492	arg1	acid					1489:1492	acetic acid	1482:1492	acetic acid	1482:1492	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	7	77	theme	acid	1489:1492	arg1	higher					1510:1515	higher	1510:1515	higher	1510:1515	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	4	78	theme	network	736:742	arg1	analysis					744:751	Co-occurrence network analysis	722:751	Co-occurrence network analysis	722:751	Co-occurrence network analysis indicated that microbiota fed with different sources of starch changed the network topological properties.					
32936008	5	79	theme	faecal	973:978	arg1	similar					998:1004	similar	998:1004	similar	998:1004	Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment.					
32936008	5	79	theme	faecal	973:978	arg1	inoculum					980:987	the original pig faecal inoculum	956:987	the original pig faecal inoculum	956:987	Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment.					
32936008	6	80	theme	maize	1066:1070	arg1	starch					1072:1077	maize starch	1066:1077	maize starch	1066:1077	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	2	81	theme	starch	447:452	arg1	sources					454:460	different starch sources	437:460	different starch sources	437:460	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	2	82	dep	human	325:329	arg1	dynamic					332:338	dynamic	332:338	dynamic	332:338	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	2	82	dep	human	325:329	arg1	TNO					361:363	TNO	361:363	TNO	361:363	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	8	83	theme	original	1716:1723	arg1	inoculum					1725:1732	original inoculum	1716:1732	original inoculum	1716:1732	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	8	83	theme	original	1716:1723	arg1	starch					1790:1795	wheat starch	1784:1795	wheat starch	1784:1795	In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX.					
32936008	1	84	dep	in	163:164	arg1	vitro					166:170	vitro	166:170	vitro	166:170	Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs.					
32936008	0	85	theme	ecological	30:39	arg1	network					41:47	ecological network	30:47	ecological network	30:47	Polysaccharide source altered ecological network, functional profile, and short-chain fatty acid production in a porcine gut microbiota.					
32936008	7	86	theme	acetic	1482:1487	arg1	acid					1489:1492	acetic acid	1482:1492	acetic acid	1482:1492	The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX.					
32936008	5	87	theme	maize	1009:1013	arg1	treatment					1022:1030	maize starch treatment	1009:1030	maize starch treatment	1009:1030	Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment.					
32936008	9	88	from	intestine	1867:1875	arg1	pigs					1888:1891	growing pigs	1880:1891	growing pigs	1880:1891	A maize basal diet may improve energy absorption in the large intestine in growing pigs.					
32936008	2	89	dep	in	365:366	arg1	vitro					368:372	vitro	368:372	vitro	368:372	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	2	90	theme	in	365:366	arg1	model					374:378	the human, dynamic, computer-controlled TNO in vitro model	321:378	the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs	321:408	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
32936008	6	91	dep	arabinoxylans	1083:1095	arg1	AX					1098:1099	AX	1098:1099	AX	1098:1099	On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG.					
32936008	3	92	theme	starch	651:656	arg1	types					642:646	three different types	626:646	three different types of starch or two polysaccharides	626:679	Our study showed that three different types of starch or two polysaccharides greatly impacted microbiota composition.					
32936008	2	93	theme	human	325:329	arg1	model					374:378	the human, dynamic, computer-controlled TNO in vitro model	321:378	the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs	321:408	This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production.					
34930890	7	0	theme	chondrogenic	1195:1206	arg1	differentiation					1208:1222	chondrogenic differentiation	1195:1222	chondrogenic differentiation	1195:1222	Finally, we found that loss of TMEM165 impairs chondrogenic differentiation by accelerating the timing of Ihh expression and promoting early chondrocyte maturation and hypertrophy.					
34930890	3	1	theme	chondroitin-and	538:552	arg1	chains					588:593	chondroitin-and heparan-sulfate glycosaminoglycan chains	538:593	chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains	538:673	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
34930890	0	2	theme	TMEM165	56:62	arg1	loss					48:51	loss	48:51	loss of TMEM165	48:62	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	0	3	theme	chondrocyte	180:190	arg1	differentiation					192:206	early chondrocyte differentiation	174:206	early chondrocyte differentiation	174:206	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	3	4	theme	heparan-sulfate	554:568	arg1	chains					588:593	chondroitin-and heparan-sulfate glycosaminoglycan chains	538:593	chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains	538:673	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
34930890	4	5	from	defect	766:771	arg1	enzymes					797:803	the Golgi elongating enzymes	776:803	the Golgi elongating enzymes	776:803	We demonstrated that the blockage in elongation of glycosaminoglycan chains is not due to defect in the Golgi elongating enzymes but rather to availability of the co-factor Mn2+.					
34930890	1	6	theme	major	288:292	arg1	dysplasia					303:311	major skeletal dysplasia	288:311	major skeletal dysplasia	288:311	TMEM165 deficiency leads to skeletal disorder characterized by major skeletal dysplasia and pronounced dwarfism.					
34930890	7	7	theme	chondrocyte	1289:1299	arg1	maturation					1301:1310	chondrocyte maturation	1289:1310	chondrocyte maturation	1289:1310	Finally, we found that loss of TMEM165 impairs chondrogenic differentiation by accelerating the timing of Ihh expression and promoting early chondrocyte maturation and hypertrophy.					
34930890	7	8	theme	TMEM165	1179:1185	arg1	loss					1171:1174	loss	1171:1174	loss of TMEM165	1171:1185	Finally, we found that loss of TMEM165 impairs chondrogenic differentiation by accelerating the timing of Ihh expression and promoting early chondrocyte maturation and hypertrophy.					
34930890	4	9	theme	co-factor	839:847	arg1	Mn2+					849:852	the co-factor Mn2+	835:852	the co-factor Mn2+	835:852	We demonstrated that the blockage in elongation of glycosaminoglycan chains is not due to defect in the Golgi elongating enzymes but rather to availability of the co-factor Mn2+.					
34930890	1	10	theme	skeletal	294:301	arg1	dysplasia					303:311	major skeletal dysplasia	288:311	major skeletal dysplasia	288:311	TMEM165 deficiency leads to skeletal disorder characterized by major skeletal dysplasia and pronounced dwarfism.					
34930890	8	11	theme	structure	1487:1495	arg1	role					1454:1457	the critical role	1441:1457	the critical role of glycosaminoglycan chains structure in the regulation of chondrogenesis	1441:1531	Collectively, our results indicate that TMEM165 plays an important role in proteoglycan synthesis and underline the critical role of glycosaminoglycan chains structure in the regulation of chondrogenesis.					
34930890	8	12	theme	critical	1445:1452	arg1	role					1454:1457	the critical role	1441:1457	the critical role of glycosaminoglycan chains structure in the regulation of chondrogenesis	1441:1531	Collectively, our results indicate that TMEM165 plays an important role in proteoglycan synthesis and underline the critical role of glycosaminoglycan chains structure in the regulation of chondrogenesis.					
34930890	6	13	theme	signaling	1058:1066	arg1	pathways					1068:1075	BMP signaling pathways	1054:1075	BMP signaling pathways	1054:1075	Additionally, we showed that TMEM165 deficiency functionally impairs TGFβ and BMP signaling pathways in chondrocytes and in fibroblast cells of TMEM165 deficient patients.					
34930890	7	14	theme	Ihh	1254:1256	arg1	expression					1258:1267	Ihh expression	1254:1267	Ihh expression	1254:1267	Finally, we found that loss of TMEM165 impairs chondrogenic differentiation by accelerating the timing of Ihh expression and promoting early chondrocyte maturation and hypertrophy.					
34930890	6	15	theme	deficient	1128:1136	arg1	patients					1138:1145	TMEM165 deficient patients	1120:1145	TMEM165 deficient patients	1120:1145	Additionally, we showed that TMEM165 deficiency functionally impairs TGFβ and BMP signaling pathways in chondrocytes and in fibroblast cells of TMEM165 deficient patients.					
34930890	5	16	with	Supplementation	855:869	arg1	Mn2+					884:887	Mn2+	884:887	Mn2+	884:887	Supplementation of cell with Mn2+ rescue the elongation process, confirming a role of TMEM165 in Mn2+ Golgi homeostasis.					
34930890	0	17	theme	chondroitin-	86:97	arg1	chains					137:142	heparan-sulfate glycosaminoglycan chains	103:142	heparan-sulfate glycosaminoglycan chains of proteoglycans	103:159	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	0	17	theme	chondroitin-	86:97	arg1	elongation					72:81	elongation	72:81	elongation of chondroitin-	72:97	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	4	18	theme	Mn2+	849:852	arg1	availability					819:830	availability	819:830	availability of the co-factor Mn2+	819:852	We demonstrated that the blockage in elongation of glycosaminoglycan chains is not due to defect in the Golgi elongating enzymes but rather to availability of the co-factor Mn2+.					
34930890	3	19	theme	proteoglycans	479:491	arg1	synthesis					466:474	the synthesis	462:474	the synthesis of proteoglycans	462:491	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
34930890	7	20	theme	expression	1258:1267	arg1	timing					1244:1249	the timing	1240:1249	the timing of Ihh expression	1240:1267	Finally, we found that loss of TMEM165 impairs chondrogenic differentiation by accelerating the timing of Ihh expression and promoting early chondrocyte maturation and hypertrophy.					
34930890	9	21	theme	deficient	1650:1658	arg1	patients					1660:1667	TMEM165 deficient patients	1642:1667	TMEM165 deficient patients	1642:1667	Our data also suggest that Mn2+ supplementation may be a promising therapeutic strategy in the treatment of TMEM165 deficient patients.					
34930890	2	22	theme	molecular	351:359	arg1	mechanisms					361:370	the molecular mechanisms	347:370	the molecular mechanisms involved	347:379	However, the molecular mechanisms involved have not been fully understood.					
34930890	5	23	theme	TMEM165	941:947	arg1	role					933:936	a role	931:936	a role of TMEM165 in Mn2+ Golgi homeostasis	931:973	Supplementation of cell with Mn2+ rescue the elongation process, confirming a role of TMEM165 in Mn2+ Golgi homeostasis.					
34930890	6	24	theme	fibroblast	1100:1109	arg1	cells					1111:1115	fibroblast cells	1100:1115	fibroblast cells of TMEM165 deficient patients	1100:1145	Additionally, we showed that TMEM165 deficiency functionally impairs TGFβ and BMP signaling pathways in chondrocytes and in fibroblast cells of TMEM165 deficient patients.					
34930890	6	25	theme	BMP	1054:1056	arg1	pathways					1068:1075	BMP signaling pathways	1054:1075	BMP signaling pathways	1054:1075	Additionally, we showed that TMEM165 deficiency functionally impairs TGFβ and BMP signaling pathways in chondrocytes and in fibroblast cells of TMEM165 deficient patients.					
34930890	9	26	from	strategy	1613:1620	arg1	treatment					1629:1637	the treatment	1625:1637	the treatment of TMEM165 deficient patients	1625:1667	Our data also suggest that Mn2+ supplementation may be a promising therapeutic strategy in the treatment of TMEM165 deficient patients.					
34930890	3	27	theme	shorter	642:648	arg1	chains					668:673	shorter glycosaminoglycan chains	642:673	shorter glycosaminoglycan chains	642:673	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
34930890	8	28	theme	glycosaminoglycan	1462:1478	arg1	chains					1480:1485	glycosaminoglycan chains	1462:1485	glycosaminoglycan chains structure	1462:1495	Collectively, our results indicate that TMEM165 plays an important role in proteoglycan synthesis and underline the critical role of glycosaminoglycan chains structure in the regulation of chondrogenesis.					
34930890	1	29	theme	pronounced	317:326	arg1	dwarfism					328:335	pronounced dwarfism	317:335	pronounced dwarfism	317:335	TMEM165 deficiency leads to skeletal disorder characterized by major skeletal dysplasia and pronounced dwarfism.					
34930890	0	30	theme	glycosaminoglycan	119:135	arg1	chains					137:142	heparan-sulfate glycosaminoglycan chains	103:142	heparan-sulfate glycosaminoglycan chains of proteoglycans	103:159	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	6	31	theme	patients	1138:1145	arg1	cells					1111:1115	fibroblast cells	1100:1115	fibroblast cells of TMEM165 deficient patients	1100:1145	Additionally, we showed that TMEM165 deficiency functionally impairs TGFβ and BMP signaling pathways in chondrocytes and in fibroblast cells of TMEM165 deficient patients.					
34930890	0	32	theme	new	10:12	arg1	player					14:19	a new player	8:19	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.	0:223	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	0	33	theme	heparan-sulfate	103:117	arg1	chains					137:142	heparan-sulfate glycosaminoglycan chains	103:142	heparan-sulfate glycosaminoglycan chains of proteoglycans	103:159	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	3	34	theme	proteoglycans	623:635	arg1	synthesis					610:618	the synthesis	606:618	the synthesis of proteoglycans with shorter glycosaminoglycan chains	606:673	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
34930890	9	35	theme	patients	1660:1667	arg1	treatment					1629:1637	the treatment	1625:1637	the treatment of TMEM165 deficient patients	1625:1667	Our data also suggest that Mn2+ supplementation may be a promising therapeutic strategy in the treatment of TMEM165 deficient patients.					
34930890	1	36	theme	TMEM165	225:231	arg1	deficiency					233:242	TMEM165 deficiency	225:242	TMEM165 deficiency	225:242	TMEM165 deficiency leads to skeletal disorder characterized by major skeletal dysplasia and pronounced dwarfism.					
34930890	0	37	theme	proteoglycan	24:35	arg1	synthesis					37:45	proteoglycan synthesis	24:45	proteoglycan synthesis	24:45	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	8	38	theme	important	1386:1394	arg1	role					1396:1399	an important role	1383:1399	an important role	1383:1399	Collectively, our results indicate that TMEM165 plays an important role in proteoglycan synthesis and underline the critical role of glycosaminoglycan chains structure in the regulation of chondrogenesis.					
34930890	9	39	theme	promising	1591:1599	arg1	supplementation					1566:1580	Mn2+ supplementation	1561:1580	Mn2+ supplementation	1561:1580	Our data also suggest that Mn2+ supplementation may be a promising therapeutic strategy in the treatment of TMEM165 deficient patients.					
34930890	9	39	theme	promising	1591:1599	arg1	strategy					1613:1620	a promising therapeutic strategy	1589:1620	a promising therapeutic strategy	1589:1620	Our data also suggest that Mn2+ supplementation may be a promising therapeutic strategy in the treatment of TMEM165 deficient patients.					
34930890	4	40	theme	glycosaminoglycan	727:743	arg1	chains					745:750	glycosaminoglycan chains	727:750	glycosaminoglycan chains	727:750	We demonstrated that the blockage in elongation of glycosaminoglycan chains is not due to defect in the Golgi elongating enzymes but rather to availability of the co-factor Mn2+.					
34930890	7	41	theme	early	1283:1287	arg1	maturation					1301:1310	chondrocyte maturation	1289:1310	chondrocyte maturation	1289:1310	Finally, we found that loss of TMEM165 impairs chondrogenic differentiation by accelerating the timing of Ihh expression and promoting early chondrocyte maturation and hypertrophy.					
34930890	5	42	theme	cell	874:877	arg1	Supplementation					855:869	Supplementation	855:869	Supplementation of cell with Mn2+	855:887	Supplementation of cell with Mn2+ rescue the elongation process, confirming a role of TMEM165 in Mn2+ Golgi homeostasis.					
34930890	5	43	theme	Mn2+	952:955	arg1	homeostasis					963:973	Mn2+ Golgi homeostasis	952:973	Mn2+ Golgi homeostasis	952:973	Supplementation of cell with Mn2+ rescue the elongation process, confirming a role of TMEM165 in Mn2+ Golgi homeostasis.					
34930890	3	44	theme	glycosaminoglycan	650:666	arg1	chains					668:673	shorter glycosaminoglycan chains	642:673	shorter glycosaminoglycan chains	642:673	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
34930890	8	45	theme	chains	1480:1485	arg1	structure					1487:1495	glycosaminoglycan chains structure	1462:1495	glycosaminoglycan chains structure	1462:1495	Collectively, our results indicate that TMEM165 plays an important role in proteoglycan synthesis and underline the critical role of glycosaminoglycan chains structure in the regulation of chondrogenesis.					
34930890	4	46	theme	elongating	786:795	arg1	enzymes					797:803	the Golgi elongating enzymes	776:803	the Golgi elongating enzymes	776:803	We demonstrated that the blockage in elongation of glycosaminoglycan chains is not due to defect in the Golgi elongating enzymes but rather to availability of the co-factor Mn2+.					
34930890	3	47	theme	TMEM165	435:441	arg1	deficiency					443:452	TMEM165 deficiency	435:452	TMEM165 deficiency	435:452	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
34930890	6	48	theme	TMEM165	1005:1011	arg1	deficiency					1013:1022	TMEM165 deficiency	1005:1022	TMEM165 deficiency	1005:1022	Additionally, we showed that TMEM165 deficiency functionally impairs TGFβ and BMP signaling pathways in chondrocytes and in fibroblast cells of TMEM165 deficient patients.					
34930890	0	49	theme	proteoglycans	147:159	arg1	chains					137:142	heparan-sulfate glycosaminoglycan chains	103:142	heparan-sulfate glycosaminoglycan chains of proteoglycans	103:159	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	0	49	theme	proteoglycans	147:159	arg1	elongation					72:81	elongation	72:81	elongation of chondroitin-	72:97	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	8	50	theme	chondrogenesis	1518:1531	arg1	regulation					1504:1513	the regulation	1500:1513	the regulation of chondrogenesis	1500:1531	Collectively, our results indicate that TMEM165 plays an important role in proteoglycan synthesis and underline the critical role of glycosaminoglycan chains structure in the regulation of chondrogenesis.					
34930890	4	51	theme	Golgi	780:784	arg1	enzymes					797:803	the Golgi elongating enzymes	776:803	the Golgi elongating enzymes	776:803	We demonstrated that the blockage in elongation of glycosaminoglycan chains is not due to defect in the Golgi elongating enzymes but rather to availability of the co-factor Mn2+.					
34930890	0	52	theme	early	174:178	arg1	differentiation					192:206	early chondrocyte differentiation	174:206	early chondrocyte differentiation	174:206	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	3	53	with	synthesis	610:618	arg1	chains					668:673	shorter glycosaminoglycan chains	642:673	shorter glycosaminoglycan chains	642:673	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
34930890	3	54	from	blockage	508:515	arg1	elongation					524:533	the elongation	520:533	the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains	520:673	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
34930890	8	55	theme	proteoglycan	1404:1415	arg1	synthesis					1417:1425	proteoglycan synthesis	1404:1425	proteoglycan synthesis	1404:1425	Collectively, our results indicate that TMEM165 plays an important role in proteoglycan synthesis and underline the critical role of glycosaminoglycan chains structure in the regulation of chondrogenesis.					
34930890	9	56	theme	Mn2+	1561:1564	arg1	supplementation					1566:1580	Mn2+ supplementation	1561:1580	Mn2+ supplementation	1561:1580	Our data also suggest that Mn2+ supplementation may be a promising therapeutic strategy in the treatment of TMEM165 deficient patients.					
34930890	9	56	theme	Mn2+	1561:1564	arg1	strategy					1613:1620	a promising therapeutic strategy	1589:1620	a promising therapeutic strategy	1589:1620	Our data also suggest that Mn2+ supplementation may be a promising therapeutic strategy in the treatment of TMEM165 deficient patients.					
34930890	5	57	theme	elongation	900:909	arg1	process					911:917	the elongation process	896:917	the elongation process	896:917	Supplementation of cell with Mn2+ rescue the elongation process, confirming a role of TMEM165 in Mn2+ Golgi homeostasis.					
34930890	5	58	theme	Golgi	957:961	arg1	homeostasis					963:973	Mn2+ Golgi homeostasis	952:973	Mn2+ Golgi homeostasis	952:973	Supplementation of cell with Mn2+ rescue the elongation process, confirming a role of TMEM165 in Mn2+ Golgi homeostasis.					
34930890	8	59	from	role	1454:1457	arg1	regulation					1504:1513	the regulation	1500:1513	the regulation of chondrogenesis	1500:1531	Collectively, our results indicate that TMEM165 plays an important role in proteoglycan synthesis and underline the critical role of glycosaminoglycan chains structure in the regulation of chondrogenesis.					
34930890	1	60	theme	skeletal	253:260	arg1	disorder					262:269	skeletal disorder	253:269	skeletal disorder characterized by major skeletal dysplasia and pronounced dwarfism	253:335	TMEM165 deficiency leads to skeletal disorder characterized by major skeletal dysplasia and pronounced dwarfism.					
34930890	4	61	from	blockage	701:708	arg1	elongation					713:722	elongation	713:722	elongation of glycosaminoglycan chains	713:750	We demonstrated that the blockage in elongation of glycosaminoglycan chains is not due to defect in the Golgi elongating enzymes but rather to availability of the co-factor Mn2+.					
34930890	9	62	theme	therapeutic	1601:1611	arg1	supplementation					1566:1580	Mn2+ supplementation	1561:1580	Mn2+ supplementation	1561:1580	Our data also suggest that Mn2+ supplementation may be a promising therapeutic strategy in the treatment of TMEM165 deficient patients.					
34930890	9	62	theme	therapeutic	1601:1611	arg1	strategy					1613:1620	a promising therapeutic strategy	1589:1620	a promising therapeutic strategy	1589:1620	Our data also suggest that Mn2+ supplementation may be a promising therapeutic strategy in the treatment of TMEM165 deficient patients.					
34930890	3	63	theme	glycosaminoglycan	570:586	arg1	chains					588:593	chondroitin-and heparan-sulfate glycosaminoglycan chains	538:593	chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains	538:673	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
34930890	4	64	theme	chains	745:750	arg1	elongation					713:722	elongation	713:722	elongation of glycosaminoglycan chains	713:750	We demonstrated that the blockage in elongation of glycosaminoglycan chains is not due to defect in the Golgi elongating enzymes but rather to availability of the co-factor Mn2+.					
34930890	0	65	from	player	14:19	arg1	synthesis					37:45	proteoglycan synthesis	24:45	proteoglycan synthesis	24:45	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	0	66	dep	TMEM165	0:6	arg1	player					14:19	a new player	8:19	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.	0:223	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	0	66	dep	TMEM165	0:6	arg1	triggers					165:172	triggers	165:172	triggers early chondrocyte differentiation and hypertrophy	165:222	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	0	66	dep	TMEM165	0:6	arg1	impairs					64:70	impairs	64:70	impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans	64:159	TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy.					
34930890	5	67	from	role	933:936	arg1	homeostasis					963:973	Mn2+ Golgi homeostasis	952:973	Mn2+ Golgi homeostasis	952:973	Supplementation of cell with Mn2+ rescue the elongation process, confirming a role of TMEM165 in Mn2+ Golgi homeostasis.					
34930890	3	68	theme	chains	588:593	arg1	elongation					524:533	the elongation	520:533	the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains	520:673	Here, we uncover that TMEM165 deficiency impairs the synthesis of proteoglycans by producing a blockage in the elongation of chondroitin-and heparan-sulfate glycosaminoglycan chains leading to the synthesis of proteoglycans with shorter glycosaminoglycan chains.					
32800953	4	0	theme	selenium	1019:1026	arg1	amount					1009:1014	a small amount	1001:1014	a small amount of selenium	1001:1026	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	4	0	theme	selenium	1019:1026	arg1	selenium					1019:1026	selenium	1019:1026	selenium	1019:1026	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	5	1	theme	rats	1167:1170	arg1	model					1172:1176	rats model	1167:1176	rats model in vivo	1167:1184	Furthermore, CdCl2 was utilized to build Cd-intoxicated cells model in vitro and rats model in vivo respectively.					
32800953	0	2	from	Characterization	0:15	arg1	platensis					70:78	Spirulina platensis	60:78	Spirulina platensis	60:78	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	8	3	theme	protective	1473:1482	arg1	role					1484:1487	protective role	1473:1487	protective role	1473:1487	The enhancement of protective role may be affected by the covalently-bonding of selenium to polysaccharide.					
32800953	6	4	theme	protective	1210:1219	arg1	effect					1221:1226	the protective effect	1206:1226	the protective effect of Se-SPP against cadmium-induced toxicity	1206:1269	Then, the protective effect of Se-SPP against cadmium-induced toxicity was assessed.					
32800953	4	5	with	heteropolysaccharide	831:850	arg1	weight					875:880	similar molecular weight	857:880	similar molecular weight	857:880	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	4	5	with	heteropolysaccharide	831:850	arg1	composition					901:911	monosaccharide composition	886:911	monosaccharide composition	886:911	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	0	6	theme	Cd-induced	112:121	arg1	toxicity					123:130	Cd-induced toxicity	112:130	Cd-induced toxicity	112:130	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	5	7	theme	Cd-intoxicated	1127:1140	arg1	model					1148:1152	Cd-intoxicated cells model	1127:1152	Cd-intoxicated cells model	1127:1152	Furthermore, CdCl2 was utilized to build Cd-intoxicated cells model in vitro and rats model in vivo respectively.					
32800953	4	8	theme	monosaccharide	886:899	arg1	composition					901:911	monosaccharide composition	886:911	monosaccharide composition	886:911	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	2	9	from	platensis	409:417	arg1	alkali-extracted					357:372	alkali-extracted	357:372	alkali-extracted	357:372	Se-SPP was alkali-extracted from selenium-containing Spirulina platensis which was cultured in Zarrouk medium supplemented with Na2SeO3.					
32800953	0	10	from	platensis	70:78	arg1	role					99:102	its protective role	84:102	its protective role against Cd-induced toxicity	84:130	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	0	10	from	platensis	70:78	arg1	Characterization					0:15	Characterization	0:15	Characterization of selenium-containing polysaccharide from Spirulina platensis	0:78	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	0	10	from	platensis	70:78	arg1	polysaccharide					40:53	selenium-containing polysaccharide	20:53	selenium-containing polysaccharide from Spirulina platensis	20:78	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	1	11	from	platensis	191:199	arg1	polysaccharide					161:174	selenium-containing polysaccharide	141:174	selenium-containing polysaccharide from Spirulina platensis (Se-SPP)	141:208	Herein, selenium-containing polysaccharide from Spirulina platensis (Se-SPP) was prepared and its structural characteristics and protective role against Cd-induced toxicity in vivo and in vitro were investigated.					
32800953	6	12	theme	Se-SPP	1231:1236	arg1	effect					1221:1226	the protective effect	1206:1226	the protective effect of Se-SPP against cadmium-induced toxicity	1206:1269	Then, the protective effect of Se-SPP against cadmium-induced toxicity was assessed.					
32800953	5	13	dep	in	1178:1179	arg1	vivo					1181:1184	vivo	1181:1184	vivo	1181:1184	Furthermore, CdCl2 was utilized to build Cd-intoxicated cells model in vitro and rats model in vivo respectively.					
32800953	7	14	theme	Cd-induced	1372:1381	arg1	toxicity					1383:1390	Cd-induced toxicity	1372:1390	Cd-induced toxicity	1372:1390	The results demonstrated that Se-SPP treatment provided significant protection against Cd-induced toxicity, which was superior compared to that of SPP or Na2SeO3 alone.					
32800953	3	15	theme	protein	513:519	arg1	contents					487:494	The contents	483:494	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP	483:696	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	4	16	theme	molecular	865:873	arg1	weight					875:880	similar molecular weight	857:880	similar molecular weight	857:880	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	4	17	theme	amount	1009:1014	arg1	covalently-bonding					979:996	the covalently-bonding	975:996	the covalently-bonding of a small amount of selenium	975:1026	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	5	18	theme	cells	1142:1146	arg1	model					1148:1152	Cd-intoxicated cells model	1127:1152	Cd-intoxicated cells model	1127:1152	Furthermore, CdCl2 was utilized to build Cd-intoxicated cells model in vitro and rats model in vivo respectively.					
32800953	4	19	theme	original	1048:1055	arg1	structure					1057:1065	the original structure	1044:1065	the original structure of polysaccharide	1044:1083	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	4	20	theme	similar	857:863	arg1	weight					875:880	similar molecular weight	857:880	similar molecular weight	857:880	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	2	21	theme	Zarrouk	441:447	arg1	medium					449:454	Zarrouk medium	441:454	Zarrouk medium supplemented with Na2SeO3	441:480	Se-SPP was alkali-extracted from selenium-containing Spirulina platensis which was cultured in Zarrouk medium supplemented with Na2SeO3.					
32800953	0	22	theme	polysaccharide	40:53	arg1	role					99:102	its protective role	84:102	its protective role against Cd-induced toxicity	84:130	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	0	22	theme	polysaccharide	40:53	arg1	Characterization					0:15	Characterization	0:15	Characterization of selenium-containing polysaccharide from Spirulina platensis	0:78	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	3	23	theme	acid	529:532	arg1	contents					487:494	The contents	483:494	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP	483:696	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	0	24	theme	selenium-containing	20:38	arg1	polysaccharide					40:53	selenium-containing polysaccharide	20:53	selenium-containing polysaccharide from Spirulina platensis	20:78	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	3	25	theme	selenium-free	722:734	arg1	SPP					752:754	SPP	752:754	SPP	752:754	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	25	theme	selenium-free	722:734	arg1	polysaccharide					736:749	selenium-free polysaccharide	722:749	selenium-free polysaccharide (SPP)	722:755	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	4	26	theme	polysaccharide	1070:1083	arg1	structure					1057:1065	the original structure	1044:1065	the original structure of polysaccharide	1044:1083	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	1	27	theme	structural	231:240	arg1	characteristics					242:256	its structural characteristics	227:256	its structural characteristics	227:256	Herein, selenium-containing polysaccharide from Spirulina platensis (Se-SPP) was prepared and its structural characteristics and protective role against Cd-induced toxicity in vivo and in vitro were investigated.					
32800953	0	28	theme	Spirulina	60:68	arg1	platensis					70:78	Spirulina platensis	60:78	Spirulina platensis	60:78	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	2	29	theme	Spirulina	399:407	arg1	platensis					409:417	selenium-containing Spirulina platensis	379:417	selenium-containing Spirulina platensis which was cultured in Zarrouk medium supplemented with Na2SeO3	379:480	Se-SPP was alkali-extracted from selenium-containing Spirulina platensis which was cultured in Zarrouk medium supplemented with Na2SeO3.					
32800953	0	30	from	role	99:102	arg1	platensis					70:78	Spirulina platensis	60:78	Spirulina platensis	60:78	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	3	31	theme	carbohydrate	499:510	arg1	contents					487:494	The contents	483:494	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP	483:696	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	7	32	theme	significant	1341:1351	arg1	protection					1353:1362	significant protection	1341:1362	significant protection against Cd-induced toxicity, which was superior compared to that of SPP or Na2SeO3 alone	1341:1451	The results demonstrated that Se-SPP treatment provided significant protection against Cd-induced toxicity, which was superior compared to that of SPP or Na2SeO3 alone.					
32800953	5	33	theme	in	1178:1179	arg1	model					1172:1176	rats model	1167:1176	rats model in vivo	1167:1184	Furthermore, CdCl2 was utilized to build Cd-intoxicated cells model in vitro and rats model in vivo respectively.					
32800953	3	34	dep	carbohydrate	499:510	arg1	N					580:580	N	580:580	N	580:580	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	34	dep	carbohydrate	499:510	arg1	S					587:587	S	587:587	S	587:587	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	34	dep	carbohydrate	499:510	arg1	O					577:577	O	577:577	O	577:577	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	34	dep	carbohydrate	499:510	arg1	H					574:574	H	574:574	H	574:574	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	34	dep	carbohydrate	499:510	arg1	C					571:571	C	571:571	C	571:571	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	35	theme	uronic	522:527	arg1	acid					529:532	uronic acid	522:532	uronic acid	522:532	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	36	theme	molecular	633:641	arg1	weight					643:648	molecular weight	633:648	molecular weight	633:648	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	4	37	theme	high-molecular-weight	809:829	arg1	heteropolysaccharide					831:850	high-molecular-weight heteropolysaccharide	809:850	high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition	809:911	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	3	38	theme	elements	547:554	arg1	contents					487:494	The contents	483:494	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP	483:696	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	5	39	used	utilized	1109:1116	arg2	CdCl2					1099:1103	CdCl2	1099:1103	CdCl2	1099:1103	Furthermore, CdCl2 was utilized to build Cd-intoxicated cells model in vitro and rats model in vivo respectively.					
32800953	6	40	theme	cadmium-induced	1246:1260	arg1	toxicity					1262:1269	cadmium-induced toxicity	1246:1269	cadmium-induced toxicity	1246:1269	Then, the protective effect of Se-SPP against cadmium-induced toxicity was assessed.					
32800953	1	41	theme	selenium-containing	141:159	arg1	polysaccharide					161:174	selenium-containing polysaccharide	141:174	selenium-containing polysaccharide from Spirulina platensis (Se-SPP)	141:208	Herein, selenium-containing polysaccharide from Spirulina platensis (Se-SPP) was prepared and its structural characteristics and protective role against Cd-induced toxicity in vivo and in vitro were investigated.					
32800953	4	42	theme	selenium	941:948	arg1	content					950:956	significantly different selenium content	917:956	significantly different selenium content	917:956	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	3	43	theme	FT-IR	674:678	arg1	spectra					680:686	FT-IR spectra	674:686	FT-IR spectra	674:686	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	44	theme	monosaccharide	605:618	arg1	composition					620:630	the monosaccharide composition	601:630	the monosaccharide composition	601:630	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	2	45	theme	selenium-containing	379:397	arg1	platensis					409:417	selenium-containing Spirulina platensis	379:417	selenium-containing Spirulina platensis which was cultured in Zarrouk medium supplemented with Na2SeO3	379:480	Se-SPP was alkali-extracted from selenium-containing Spirulina platensis which was cultured in Zarrouk medium supplemented with Na2SeO3.					
32800953	1	46	theme	protective	262:271	arg1	role					273:276	protective role	262:276	protective role against Cd-induced toxicity in vivo and in vitro	262:325	Herein, selenium-containing polysaccharide from Spirulina platensis (Se-SPP) was prepared and its structural characteristics and protective role against Cd-induced toxicity in vivo and in vitro were investigated.					
32800953	4	47	theme	different	931:939	arg1	content					950:956	significantly different selenium content	917:956	significantly different selenium content	917:956	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	3	48	theme	surface	651:657	arg1	morphology					659:668	surface morphology	651:668	surface morphology	651:668	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	49	theme	composition	620:630	arg1	contents					487:494	The contents	483:494	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP	483:696	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	7	50	theme	Se-SPP	1315:1320	arg1	treatment					1322:1330	Se-SPP treatment	1315:1330	Se-SPP treatment	1315:1330	The results demonstrated that Se-SPP treatment provided significant protection against Cd-induced toxicity, which was superior compared to that of SPP or Na2SeO3 alone.					
32800953	3	51	theme	sulfate	535:541	arg1	contents					487:494	The contents	483:494	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP	483:696	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	0	52	theme	protective	88:97	arg1	role					99:102	its protective role	84:102	its protective role against Cd-induced toxicity	84:130	Characterization of selenium-containing polysaccharide from Spirulina platensis and its protective role against Cd-induced toxicity.					
32800953	8	53	theme	selenium	1534:1541	arg1	covalently-bonding					1512:1529	the covalently-bonding	1508:1529	the covalently-bonding of selenium to polysaccharide	1508:1559	The enhancement of protective role may be affected by the covalently-bonding of selenium to polysaccharide.					
32800953	1	54	theme	Spirulina	181:189	arg1	platensis					191:199	Spirulina platensis	181:199	Spirulina platensis (Se-SPP)	181:208	Herein, selenium-containing polysaccharide from Spirulina platensis (Se-SPP) was prepared and its structural characteristics and protective role against Cd-induced toxicity in vivo and in vitro were investigated.					
32800953	1	54	theme	Spirulina	181:189	arg1	Se-SPP					202:207	Se-SPP	202:207	Se-SPP	202:207	Herein, selenium-containing polysaccharide from Spirulina platensis (Se-SPP) was prepared and its structural characteristics and protective role against Cd-induced toxicity in vivo and in vitro were investigated.					
32800953	4	55	theme	small	1003:1007	arg1	amount					1009:1014	a small amount	1001:1014	a small amount of selenium	1001:1026	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	4	55	theme	small	1003:1007	arg1	selenium					1019:1026	selenium	1019:1026	selenium	1019:1026	The results revealed that SPP and Se-SPP were both high-molecular-weight heteropolysaccharide with similar molecular weight and monosaccharide composition but significantly different selenium content, indicating that the covalently-bonding of a small amount of selenium did not destroy the original structure of polysaccharide.					
32800953	3	56	theme	Se-SPP	691:696	arg1	carbohydrate					499:510	carbohydrate	499:510	carbohydrate	499:510	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	56	theme	Se-SPP	691:696	arg1	weight					643:648	molecular weight	633:648	molecular weight	633:648	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	56	theme	Se-SPP	691:696	arg1	sulfate					535:541	sulfate	535:541	sulfate	535:541	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	56	theme	Se-SPP	691:696	arg1	protein					513:519	protein	513:519	protein	513:519	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	56	theme	Se-SPP	691:696	arg1	elements					547:554	elements	547:554	elements	547:554	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	56	theme	Se-SPP	691:696	arg1	composition					620:630	the monosaccharide composition	601:630	the monosaccharide composition	601:630	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	56	theme	Se-SPP	691:696	arg1	morphology					659:668	surface morphology	651:668	surface morphology	651:668	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	56	theme	Se-SPP	691:696	arg1	acid					529:532	uronic acid	522:532	uronic acid	522:532	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	3	56	theme	Se-SPP	691:696	arg1	spectra					680:686	FT-IR spectra	674:686	FT-IR spectra	674:686	The contents of carbohydrate, protein, uronic acid, sulfate and elements (including Se, C, H, O, N, and S) as well as the monosaccharide composition, molecular weight, surface morphology and FT-IR spectra of Se-SPP was compared to that of selenium-free polysaccharide (SPP).					
32800953	8	57	theme	role	1484:1487	arg1	enhancement					1458:1468	The enhancement	1454:1468	The enhancement of protective role	1454:1487	The enhancement of protective role may be affected by the covalently-bonding of selenium to polysaccharide.					
32800953	1	58	theme	Cd-induced	286:295	arg1	toxicity					297:304	Cd-induced toxicity	286:304	Cd-induced toxicity	286:304	Herein, selenium-containing polysaccharide from Spirulina platensis (Se-SPP) was prepared and its structural characteristics and protective role against Cd-induced toxicity in vivo and in vitro were investigated.					
34491587	3	0	theme	known	323:327	arg1	velia					361:365	velia	361:365	velia	361:365	There are two known species of chromerids: Chromera velia and Vitrella brassicaformis.					
34491587	3	0	theme	known	323:327	arg1	species					329:335	two known species	319:335	two known species of chromerids: Chromera velia and Vitrella brassicaformis	319:393	There are two known species of chromerids: Chromera velia and Vitrella brassicaformis.					
34491587	3	0	theme	known	323:327	arg1	brassicaformis					380:393	brassicaformis	380:393	brassicaformis	380:393	There are two known species of chromerids: Chromera velia and Vitrella brassicaformis.					
34491587	9	1	theme	mixture	907:913	arg1	polysaccharides					811:825	The remaining wall polysaccharides	792:825	The remaining wall polysaccharides	792:825	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	9	1	theme	mixture	907:913	arg1	similar					852:858	similar	852:858	similar	852:858	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	9	1	theme	mixture	907:913	arg1	indicative					891:900	indicative	891:900	indicative	891:900	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	9	2	theme	remaining	796:804	arg1	polysaccharides					811:825	The remaining wall polysaccharides	792:825	The remaining wall polysaccharides	792:825	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	9	2	theme	remaining	796:804	arg1	similar					852:858	similar	852:858	similar	852:858	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	9	2	theme	remaining	796:804	arg1	indicative					891:900	indicative	891:900	indicative	891:900	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	5	3	theme	abundant	544:551	arg1	monosaccharide					553:566	the most abundant monosaccharide	535:566	the most abundant monosaccharide (approx	535:574	Several polysaccharides adorn this structure, with glucose being the most abundant monosaccharide (approx.					
34491587	5	3	theme	abundant	544:551	arg1	glucose					521:527	glucose	521:527	glucose	521:527	Several polysaccharides adorn this structure, with glucose being the most abundant monosaccharide (approx.					
34491587	10	4	theme	alveolate	1079:1087	arg1	C. velia					1089:1096	the photosynthetic alveolate C. velia	1060:1096	the photosynthetic alveolate C. velia	1060:1096	The present work provides, for the first time, insights into the outermost layers of the photosynthetic alveolate C. velia.					
34491587	8	5	theme	cell	786:789	arg1	staining					764:771	calcofluor white staining	747:771	calcofluor white staining of the algal cell	747:789	The presence of cellulose was cytochemically confirmed with calcofluor white staining of the algal cell.					
34491587	10	6	theme	outermost	1040:1048	arg1	layers					1050:1055	the outermost layers	1036:1055	the outermost layers of the photosynthetic alveolate C. velia	1036:1096	The present work provides, for the first time, insights into the outermost layers of the photosynthetic alveolate C. velia.					
34491587	7	7	theme	chromerids	644:653	arg1	wall					660:663	the chromerids cell wall	640:663	the chromerids cell wall	640:663	60%), suggesting that the chromerids cell wall is mostly cellulosic.					
34491587	7	7	theme	chromerids	644:653	arg1	cellulosic					675:684	cellulosic	675:684	cellulosic	675:684	60%), suggesting that the chromerids cell wall is mostly cellulosic.					
34491587	8	8	theme	algal	780:784	arg1	cell					786:789	the algal cell	776:789	the algal cell	776:789	The presence of cellulose was cytochemically confirmed with calcofluor white staining of the algal cell.					
34491587	9	9	theme	galactans	918:926	arg1	mixture					907:913	a mixture	905:913	a mixture of galactans, xyloglucans, heteroxylans, and heteromannans	905:972	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	10	10	theme	present	979:985	arg1	work					987:990	The present work	975:990	The present work	975:990	The present work provides, for the first time, insights into the outermost layers of the photosynthetic alveolate C. velia.					
34491587	1	11	located	found	131:135	arg2	Chromerids					93:102	Chromerids	93:102	Chromerids	93:102	Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features.					
34491587	1	11	located	found	131:135	arg2	alveolates					119:128	alveolates	119:128	alveolates	119:128	Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features.					
34491587	1	11	located	found	131:135	arg2	group					110:114	a group	108:114	a group	108:114	Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features.					
34491587	1	11	located	found	131:135	arg1	corals					140:145	corals	140:145	corals	140:145	Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features.					
34491587	2	12	theme	parasitic	284:292	arg1	apicomplexans					294:306	parasitic apicomplexans	284:306	parasitic apicomplexans	284:306	These organisms are evolutionary placed in-between symbiotic dinoflagellates and parasitic apicomplexans.					
34491587	9	13	theme	xyloglucans	929:939	arg1	mixture					907:913	a mixture	905:913	a mixture of galactans, xyloglucans, heteroxylans, and heteromannans	905:972	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	10	14	theme	photosynthetic	1064:1077	arg1	C. velia					1089:1096	the photosynthetic alveolate C. velia	1060:1096	the photosynthetic alveolate C. velia	1060:1096	The present work provides, for the first time, insights into the outermost layers of the photosynthetic alveolate C. velia.					
34491587	4	15	theme	biochemical	406:416	arg1	composition					418:428	the biochemical composition	402:428	the biochemical composition of the C. velia cell wall	402:454	Here, the biochemical composition of the C. velia cell wall was analyzed.					
34491587	0	16	dep	velia	86:90	arg1	polysaccharides					14:28	The cell wall polysaccharides	0:28	The cell wall polysaccharides of a photosynthetic relative of apicomplexans	0:74	The cell wall polysaccharides of a photosynthetic relative of apicomplexans, Chromera velia.					
34491587	9	17	theme	heteromannans	960:972	arg1	mixture					907:913	a mixture	905:913	a mixture of galactans, xyloglucans, heteroxylans, and heteromannans	905:972	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	0	18	theme	cell	4:7	arg1	polysaccharides					14:28	The cell wall polysaccharides	0:28	The cell wall polysaccharides of a photosynthetic relative of apicomplexans	0:74	The cell wall polysaccharides of a photosynthetic relative of apicomplexans, Chromera velia.					
34491587	10	19	theme	C. velia	1089:1096	arg1	layers					1050:1055	the outermost layers	1036:1055	the outermost layers of the photosynthetic alveolate C. velia	1036:1096	The present work provides, for the first time, insights into the outermost layers of the photosynthetic alveolate C. velia.					
34491587	7	20	theme	cell	655:658	arg1	wall					660:663	the chromerids cell wall	640:663	the chromerids cell wall	640:663	60%), suggesting that the chromerids cell wall is mostly cellulosic.					
34491587	7	20	theme	cell	655:658	arg1	cellulosic					675:684	cellulosic	675:684	cellulosic	675:684	60%), suggesting that the chromerids cell wall is mostly cellulosic.					
34491587	5	21	theme	Several	470:476	arg1	polysaccharides					478:492	Several polysaccharides	470:492	Several polysaccharides	470:492	Several polysaccharides adorn this structure, with glucose being the most abundant monosaccharide (approx.					
34491587	1	22	theme	peculiar	158:165	arg1	features					193:200	peculiar morphological and genomic features	158:200	peculiar morphological and genomic features	158:200	Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features.					
34491587	1	23	theme	morphological	167:179	arg1	features					193:200	peculiar morphological and genomic features	158:200	peculiar morphological and genomic features	158:200	Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features.					
34491587	0	24	theme	wall	9:12	arg1	polysaccharides					14:28	The cell wall polysaccharides	0:28	The cell wall polysaccharides of a photosynthetic relative of apicomplexans	0:74	The cell wall polysaccharides of a photosynthetic relative of apicomplexans, Chromera velia.					
34491587	3	25	dep	species	329:335	arg1	velia					361:365	velia	361:365	velia	361:365	There are two known species of chromerids: Chromera velia and Vitrella brassicaformis.					
34491587	3	25	dep	species	329:335	arg1	brassicaformis					380:393	brassicaformis	380:393	brassicaformis	380:393	There are two known species of chromerids: Chromera velia and Vitrella brassicaformis.					
34491587	3	25	dep	species	329:335	arg1	species					329:335	two known species	319:335	two known species of chromerids: Chromera velia and Vitrella brassicaformis	319:393	There are two known species of chromerids: Chromera velia and Vitrella brassicaformis.					
34491587	8	26	theme	cellulose	703:711	arg1	presence					691:698	The presence	687:698	The presence of cellulose	687:711	The presence of cellulose was cytochemically confirmed with calcofluor white staining of the algal cell.					
34491587	4	27	theme	wall	451:454	arg1	composition					418:428	the biochemical composition	402:428	the biochemical composition of the C. velia cell wall	402:454	Here, the biochemical composition of the C. velia cell wall was analyzed.					
34491587	10	28	theme	first	1010:1014	arg1	time					1016:1019	the first time	1006:1019	the first time	1006:1019	The present work provides, for the first time, insights into the outermost layers of the photosynthetic alveolate C. velia.					
34491587	1	29	theme	genomic	185:191	arg1	features					193:200	peculiar morphological and genomic features	158:200	peculiar morphological and genomic features	158:200	Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features.					
34491587	9	30	dep	similar	852:858	arg1	polysaccharides					811:825	The remaining wall polysaccharides	792:825	The remaining wall polysaccharides	792:825	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	9	30	dep	similar	852:858	arg1	similar					852:858	similar	852:858	similar	852:858	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	9	30	dep	similar	852:858	arg1	indicative					891:900	indicative	891:900	indicative	891:900	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	9	31	theme	higher	872:877	arg1	plants					879:884	higher plants	872:884	higher plants	872:884	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	0	32	theme	relative	50:57	arg1	polysaccharides					14:28	The cell wall polysaccharides	0:28	The cell wall polysaccharides of a photosynthetic relative of apicomplexans	0:74	The cell wall polysaccharides of a photosynthetic relative of apicomplexans, Chromera velia.					
34491587	0	33	theme	photosynthetic	35:48	arg1	relative					50:57	a photosynthetic relative	33:57	a photosynthetic relative of apicomplexans	33:74	The cell wall polysaccharides of a photosynthetic relative of apicomplexans, Chromera velia.					
34491587	2	34	theme	symbiotic	254:262	arg1	organisms					209:217	These organisms	203:217	These organisms	203:217	These organisms are evolutionary placed in-between symbiotic dinoflagellates and parasitic apicomplexans.					
34491587	2	34	theme	symbiotic	254:262	arg1	dinoflagellates					264:278	evolutionary placed in-between symbiotic dinoflagellates	223:278	evolutionary placed in-between symbiotic dinoflagellates	223:278	These organisms are evolutionary placed in-between symbiotic dinoflagellates and parasitic apicomplexans.					
34491587	9	35	theme	wall	806:809	arg1	polysaccharides					811:825	The remaining wall polysaccharides	792:825	The remaining wall polysaccharides	792:825	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	9	35	theme	wall	806:809	arg1	similar					852:858	similar	852:858	similar	852:858	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	9	35	theme	wall	806:809	arg1	indicative					891:900	indicative	891:900	indicative	891:900	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34491587	4	36	theme	cell	446:449	arg1	wall					451:454	the C. velia cell wall	433:454	the C. velia cell wall	433:454	Here, the biochemical composition of the C. velia cell wall was analyzed.					
34491587	2	37	theme	in-between	243:252	arg1	organisms					209:217	These organisms	203:217	These organisms	203:217	These organisms are evolutionary placed in-between symbiotic dinoflagellates and parasitic apicomplexans.					
34491587	2	37	theme	in-between	243:252	arg1	dinoflagellates					264:278	evolutionary placed in-between symbiotic dinoflagellates	223:278	evolutionary placed in-between symbiotic dinoflagellates	223:278	These organisms are evolutionary placed in-between symbiotic dinoflagellates and parasitic apicomplexans.					
34491587	1	38	theme	alveolates	119:128	arg1	alveolates					119:128	alveolates	119:128	alveolates	119:128	Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features.					
34491587	1	38	theme	alveolates	119:128	arg1	Chromerids					93:102	Chromerids	93:102	Chromerids	93:102	Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features.					
34491587	1	38	theme	alveolates	119:128	arg1	group					110:114	a group	108:114	a group	108:114	Chromerids are a group of alveolates, found in corals, that show peculiar morphological and genomic features.					
34491587	8	39	theme	white	758:762	arg1	staining					764:771	calcofluor white staining	747:771	calcofluor white staining of the algal cell	747:789	The presence of cellulose was cytochemically confirmed with calcofluor white staining of the algal cell.					
34491587	0	40	theme	apicomplexans	62:74	arg1	relative					50:57	a photosynthetic relative	33:57	a photosynthetic relative of apicomplexans	33:74	The cell wall polysaccharides of a photosynthetic relative of apicomplexans, Chromera velia.					
34491587	4	41	theme	C. velia	437:444	arg1	wall					451:454	the C. velia cell wall	433:454	the C. velia cell wall	433:454	Here, the biochemical composition of the C. velia cell wall was analyzed.					
34491587	2	42	theme	placed	236:241	arg1	organisms					209:217	These organisms	203:217	These organisms	203:217	These organisms are evolutionary placed in-between symbiotic dinoflagellates and parasitic apicomplexans.					
34491587	2	42	theme	placed	236:241	arg1	dinoflagellates					264:278	evolutionary placed in-between symbiotic dinoflagellates	223:278	evolutionary placed in-between symbiotic dinoflagellates	223:278	These organisms are evolutionary placed in-between symbiotic dinoflagellates and parasitic apicomplexans.					
34491587	3	43	theme	chromerids	340:349	arg1	velia					361:365	velia	361:365	velia	361:365	There are two known species of chromerids: Chromera velia and Vitrella brassicaformis.					
34491587	3	43	theme	chromerids	340:349	arg1	species					329:335	two known species	319:335	two known species of chromerids: Chromera velia and Vitrella brassicaformis	319:393	There are two known species of chromerids: Chromera velia and Vitrella brassicaformis.					
34491587	3	43	theme	chromerids	340:349	arg1	brassicaformis					380:393	brassicaformis	380:393	brassicaformis	380:393	There are two known species of chromerids: Chromera velia and Vitrella brassicaformis.					
34491587	2	44	theme	evolutionary	223:234	arg1	organisms					209:217	These organisms	203:217	These organisms	203:217	These organisms are evolutionary placed in-between symbiotic dinoflagellates and parasitic apicomplexans.					
34491587	2	44	theme	evolutionary	223:234	arg1	dinoflagellates					264:278	evolutionary placed in-between symbiotic dinoflagellates	223:278	evolutionary placed in-between symbiotic dinoflagellates	223:278	These organisms are evolutionary placed in-between symbiotic dinoflagellates and parasitic apicomplexans.					
34491587	9	45	theme	heteroxylans	942:953	arg1	mixture					907:913	a mixture	905:913	a mixture of galactans, xyloglucans, heteroxylans, and heteromannans	905:972	The remaining wall polysaccharides, assuming structures are similar to those of higher plants, are indicative of a mixture of galactans, xyloglucans, heteroxylans, and heteromannans.					
34380178	5	0	theme	plant	738:742	arg1	wall					749:752	the plant cell wall	734:752	the plant cell wall	734:752	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	6	1	theme	kjc1	936:939	arg1	mucilage					924:931	adherent mucilage	915:931	adherent mucilage of kjc1 and vtc1 mutants	915:956	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	7	2	contain	had	1205:1207	arg1	galactoglucomannan					1186:1203	galactoglucomannan	1186:1203	galactoglucomannan	1186:1203	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	7	2	contain	had	1205:1207	arg2	backbone					1233:1240	a patterned glucomannan backbone	1209:1240	a patterned glucomannan backbone consisting of alternating residues of glucose and mannose	1209:1298	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	7	3	theme	patterned	1211:1219	arg1	backbone					1233:1240	a patterned glucomannan backbone	1209:1240	a patterned glucomannan backbone consisting of alternating residues of glucose and mannose	1209:1298	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	6	4	theme	composition	863:873	arg1	analysis					875:882	Sugar composition analysis	857:882	Sugar composition analysis	857:882	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	5	5	theme	cell	744:747	arg1	wall					749:752	the plant cell wall	734:752	the plant cell wall	734:752	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	0	6	from	structure	19:27	arg1	synthesis					78:86	GDP-mannose synthesis	66:86	GDP-mannose synthesis	66:86	Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis impaired mutants.					
34380178	7	7	theme	other	1071:1075	arg1	hand					1077:1080	the other hand	1067:1080	the other hand	1067:1080	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	6	8	theme	drastic	1024:1030	arg1	decrease					1032:1039	a drastic decrease	1022:1039	a drastic decrease of galactoglucomannan	1022:1061	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	5	9	theme	seed-coat	691:699	arg1	model					725:729	a model	723:729	a model of the plant cell wall	723:752	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	5	9	theme	seed-coat	691:699	arg1	mucilage					701:708	seed-coat mucilage	691:708	seed-coat mucilage	691:708	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	7	10	theme	structural	1083:1092	arg1	analysis					1094:1101	structural analysis	1083:1101	structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase	1083:1169	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	6	11	theme	mutants	950:956	arg1	mucilage					924:931	adherent mucilage	915:931	adherent mucilage of kjc1 and vtc1 mutants	915:956	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	6	12	theme	Sugar	857:861	arg1	analysis					875:882	Sugar composition analysis	857:882	Sugar composition analysis	857:882	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	5	13	theme	kjc1	793:796	arg1	mutations					798:806	vtc1 and kjc1 mutations	784:806	vtc1 and kjc1 mutations	784:806	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	6	14	theme	wild-type	986:994	arg1	wild-type					986:994	wild-type	986:994	wild-type	986:994	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	6	14	theme	wild-type	986:994	arg1	%					969:969	only 42%	962:969	only 42%	962:969	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	6	14	theme	wild-type	986:994	arg1	%					977:977	11%	975:977	11% of the wild-type	975:994	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	3	15	from	sugars	393:398	arg1	plants					403:408	plants	403:408	plants	403:408	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	5	16	theme	wall	749:752	arg1	model					725:729	a model	723:729	a model of the plant cell wall	723:752	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	5	16	theme	wall	749:752	arg1	mucilage					701:708	seed-coat mucilage	691:708	seed-coat mucilage	691:708	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	8	17	theme	availability	1535:1546	arg1	properties					1484:1493	properties	1484:1493	properties of glycosyltransferases rather than the availability of nucleotide sugars	1484:1567	These results suggest that the structure of mucilage galactoglucomannan is mainly determined by properties of glycosyltransferases rather than the availability of nucleotide sugars.					
34380178	4	18	from	KONJAC1	654:660	arg1	Arabidopsis					672:682	Arabidopsis	672:682	Arabidopsis	672:682	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	4	19	theme	protein	646:652	arg1	KONJAC1					654:660	the interacting protein KONJAC1	630:660	the interacting protein KONJAC1 (KJC1) in Arabidopsis	630:682	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	4	19	theme	protein	646:652	arg1	KJC1					663:666	KJC1	663:666	KJC1	663:666	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	3	20	theme	sugars	393:398	arg1	sugars					393:398	the major nucleotide sugars	372:398	the major nucleotide sugars in plants	372:408	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	3	20	theme	sugars	393:398	arg1	one					365:367	one	365:367	one	365:367	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	5	21	from	influence	771:779	arg1	synthesis					815:823	the synthesis	811:823	the synthesis of mucilage galactoglucomannan	811:854	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	7	22	theme	glucose	1280:1286	arg1	mannose					1292:1298	mannose	1292:1298	mannose	1292:1298	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	7	22	theme	glucose	1280:1286	arg1	glucose					1280:1286	glucose	1280:1286	glucose	1280:1286	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	7	22	theme	glucose	1280:1286	arg1	residues					1268:1275	alternating residues	1256:1275	alternating residues of glucose and mannose	1256:1298	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	1	23	theme	Cell-wall	106:114	arg1	polysaccharides					116:130	Cell-wall polysaccharides	106:130	Cell-wall polysaccharides	106:130	Cell-wall polysaccharides are synthesized from nucleotide sugars by glycosyltransferases.					
34380178	6	24	from	content	904:910	arg1	mucilage					924:931	adherent mucilage	915:931	adherent mucilage of kjc1 and vtc1 mutants	915:956	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	0	25	theme	Galactoglucomannan	0:17	arg1	structure					19:27	Galactoglucomannan structure	0:27	Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis	0:86	Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis impaired mutants.					
34380178	6	26	theme	vtc1	945:948	arg1	mutants					950:956	vtc1 mutants	945:956	vtc1 mutants	945:956	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	5	27	theme	mucilage	828:835	arg1	galactoglucomannan					837:854	mucilage galactoglucomannan	828:854	mucilage galactoglucomannan	828:854	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	3	28	theme	major	376:380	arg1	sugars					393:398	the major nucleotide sugars	372:398	the major nucleotide sugars in plants	372:408	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	7	29	theme	branches	1334:1341	arg1	frequency					1308:1316	the frequency	1304:1316	the frequency of α-galactosyl branches	1304:1341	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	7	29	theme	branches	1334:1341	arg1	similar					1352:1358	similar	1352:1358	similar	1352:1358	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	8	30	theme	galactoglucomannan	1441:1458	arg1	structure					1419:1427	the structure	1415:1427	the structure of mucilage galactoglucomannan	1415:1458	These results suggest that the structure of mucilage galactoglucomannan is mainly determined by properties of glycosyltransferases rather than the availability of nucleotide sugars.					
34380178	8	31	theme	glycosyltransferases	1498:1517	arg1	properties					1484:1493	properties	1484:1493	properties of glycosyltransferases rather than the availability of nucleotide sugars	1484:1567	These results suggest that the structure of mucilage galactoglucomannan is mainly determined by properties of glycosyltransferases rather than the availability of nucleotide sugars.					
34380178	3	32	theme	mannan	456:461	arg1	polysaccharides					463:477	mannan polysaccharides	456:477	mannan polysaccharides	456:477	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	3	33	theme	nucleotide	382:391	arg1	sugars					393:398	the major nucleotide sugars	372:398	the major nucleotide sugars in plants	372:408	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	4	34	theme	C	576:576	arg1	VTC1					590:593	VTC1	590:593	VTC1	590:593	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	4	34	theme	C	576:576	arg1	DEFECTIVE1					578:587	VITAMIN C DEFECTIVE1	568:587	VITAMIN C DEFECTIVE1 (VTC1)	568:594	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	4	34	theme	C	576:576	arg1	pyrophosphorylase					549:565	a GDP-Man pyrophosphorylase	539:565	a GDP-Man pyrophosphorylase	539:565	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	3	35	theme	guanosine	316:324	arg1	GDP					339:341	GDP	339:341	GDP	339:341	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	3	35	theme	guanosine	316:324	arg1	diphosphate					326:336	guanosine diphosphate	316:336	guanosine diphosphate (GDP)-mannose (GDP-Man)	316:360	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	8	36	theme	mucilage	1432:1439	arg1	galactoglucomannan					1441:1458	mucilage galactoglucomannan	1432:1458	mucilage galactoglucomannan	1432:1458	These results suggest that the structure of mucilage galactoglucomannan is mainly determined by properties of glycosyltransferases rather than the availability of nucleotide sugars.					
34380178	7	37	theme	glucomannan	1221:1231	arg1	backbone					1233:1240	a patterned glucomannan backbone	1209:1240	a patterned glucomannan backbone consisting of alternating residues of glucose and mannose	1209:1298	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	3	38	theme	polysaccharides	463:477	arg1	synthesis					443:451	the synthesis	439:451	the synthesis of mannan polysaccharides	439:477	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	0	39	theme	mucilage	54:61	arg1	structure					19:27	Galactoglucomannan structure	0:27	Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis	0:86	Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis impaired mutants.					
34380178	4	40	theme	VITAMIN	568:574	arg1	VTC1					590:593	VTC1	590:593	VTC1	590:593	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	4	40	theme	VITAMIN	568:574	arg1	DEFECTIVE1					578:587	VITAMIN C DEFECTIVE1	568:587	VITAMIN C DEFECTIVE1 (VTC1)	568:594	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	4	40	theme	VITAMIN	568:574	arg1	pyrophosphorylase					549:565	a GDP-Man pyrophosphorylase	539:565	a GDP-Man pyrophosphorylase	539:565	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	5	41	theme	mutations	798:806	arg1	influence					771:779	the influence	767:779	the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan	767:854	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	7	42	theme	wild	1367:1370	arg1	structure					1377:1385	the wild type structure	1363:1385	the wild type structure	1363:1385	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	4	43	theme	interacting	634:644	arg1	KONJAC1					654:660	the interacting protein KONJAC1	630:660	the interacting protein KONJAC1 (KJC1) in Arabidopsis	630:682	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	4	43	theme	interacting	634:644	arg1	KJC1					663:666	KJC1	663:666	KJC1	663:666	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	0	44	theme	seed-coat	44:52	arg1	mucilage					54:61	Arabidopsis seed-coat mucilage	32:61	Arabidopsis seed-coat mucilage	32:61	Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis impaired mutants.					
34380178	0	45	theme	Arabidopsis	32:42	arg1	mucilage					54:61	Arabidopsis seed-coat mucilage	32:61	Arabidopsis seed-coat mucilage	32:61	Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis impaired mutants.					
34380178	0	46	theme	GDP-mannose	66:76	arg1	synthesis					78:86	GDP-mannose synthesis	66:86	GDP-mannose synthesis	66:86	Galactoglucomannan structure of Arabidopsis seed-coat mucilage in GDP-mannose synthesis impaired mutants.					
34380178	6	47	theme	adherent	915:922	arg1	mucilage					924:931	adherent mucilage	915:931	adherent mucilage of kjc1 and vtc1 mutants	915:956	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	8	48	theme	sugars	1562:1567	arg1	availability					1535:1546	the availability	1531:1546	glycosyltransferases rather than the availability of nucleotide sugars	1498:1567	These results suggest that the structure of mucilage galactoglucomannan is mainly determined by properties of glycosyltransferases rather than the availability of nucleotide sugars.					
34380178	6	49	theme	galactoglucomannan	1044:1061	arg1	decrease					1032:1039	a drastic decrease	1022:1039	a drastic decrease of galactoglucomannan	1022:1061	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	8	50	theme	nucleotide	1551:1560	arg1	sugars					1562:1567	nucleotide sugars	1551:1567	nucleotide sugars	1551:1567	These results suggest that the structure of mucilage galactoglucomannan is mainly determined by properties of glycosyltransferases rather than the availability of nucleotide sugars.					
34380178	4	51	theme	GDP-Man	541:547	arg1	DEFECTIVE1					578:587	VITAMIN C DEFECTIVE1	568:587	VITAMIN C DEFECTIVE1 (VTC1)	568:594	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	4	51	theme	GDP-Man	541:547	arg1	pyrophosphorylase					549:565	a GDP-Man pyrophosphorylase	539:565	a GDP-Man pyrophosphorylase	539:565	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	6	52	theme	mannose	896:902	arg1	content					904:910	mannose content	896:910	mannose content in adherent mucilage of kjc1 and vtc1 mutants	896:956	Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild-type, respectively, indicating a drastic decrease of galactoglucomannan.					
34380178	2	53	theme	sugars	241:246	arg1	level					221:225	the level	217:225	the level of nucleotide sugars	217:246	However, in what way the level of nucleotide sugars affects the structure of the polysaccharides is not entirely clear.					
34380178	7	54	theme	α-galactosyl	1321:1332	arg1	branches					1334:1341	α-galactosyl branches	1321:1341	α-galactosyl branches	1321:1341	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	1	55	theme	nucleotide	153:162	arg1	sugars					164:169	nucleotide sugars	153:169	nucleotide sugars	153:169	Cell-wall polysaccharides are synthesized from nucleotide sugars by glycosyltransferases.					
34380178	3	56	theme	diphosphate	326:336	arg1	GDP-Man					353:359	GDP-Man	353:359	GDP-Man	353:359	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	3	56	theme	diphosphate	326:336	arg1	-mannose					343:350	guanosine diphosphate (GDP)-mannose	316:350	guanosine diphosphate (GDP)-mannose (GDP-Man)	316:360	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	3	56	theme	diphosphate	326:336	arg1	substrate					426:434	a substrate	424:434	a substrate in the synthesis of mannan polysaccharides	424:477	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	2	57	theme	nucleotide	230:239	arg1	sugars					241:246	nucleotide sugars	230:246	nucleotide sugars	230:246	However, in what way the level of nucleotide sugars affects the structure of the polysaccharides is not entirely clear.					
34380178	7	58	theme	type	1372:1375	arg1	structure					1377:1385	the wild type structure	1363:1385	the wild type structure	1363:1385	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	7	59	theme	alternating	1256:1266	arg1	mannose					1292:1298	mannose	1292:1298	mannose	1292:1298	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	7	59	theme	alternating	1256:1266	arg1	glucose					1280:1286	glucose	1280:1286	glucose	1280:1286	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	7	59	theme	alternating	1256:1266	arg1	residues					1268:1275	alternating residues	1256:1275	alternating residues of glucose and mannose	1256:1298	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	2	60	theme	polysaccharides	277:291	arg1	structure					260:268	the structure	256:268	the structure of the polysaccharides	256:291	However, in what way the level of nucleotide sugars affects the structure of the polysaccharides is not entirely clear.					
34380178	3	61	from	substrate	426:434	arg1	synthesis					443:451	the synthesis	439:451	the synthesis of mannan polysaccharides	439:477	guanosine diphosphate (GDP)-mannose (GDP-Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides.					
34380178	5	62	theme	galactoglucomannan	837:854	arg1	synthesis					815:823	the synthesis	811:823	the synthesis of mucilage galactoglucomannan	811:854	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	7	63	theme	mannose	1292:1298	arg1	mannose					1292:1298	mannose	1292:1298	mannose	1292:1298	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	7	63	theme	mannose	1292:1298	arg1	glucose					1280:1286	glucose	1280:1286	glucose	1280:1286	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	7	63	theme	mannose	1292:1298	arg1	residues					1268:1275	alternating residues	1256:1275	alternating residues of glucose and mannose	1256:1298	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
34380178	4	64	theme	mannose	508:514	arg1	1-phosphate					516:526	mannose 1-phosphate	508:526	mannose 1-phosphate	508:526	GDP-Man is synthesized from mannose 1-phosphate and GTP by a GDP-Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis.					
34380178	5	65	theme	vtc1	784:787	arg1	mutations					798:806	vtc1 and kjc1 mutations	784:806	vtc1 and kjc1 mutations	784:806	Since seed-coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan.					
34380178	7	66	theme	specific	1112:1119	arg1	oligosaccharides					1121:1136	specific oligosaccharides	1112:1136	specific oligosaccharides released by endo-β-1,4-mannanase	1112:1169	On the other hand, structural analysis based on specific oligosaccharides released by endo-β-1,4-mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α-galactosyl branches was also similar to the wild type structure.					
32213731	4	0	theme	fundamental	850:860	arg1	data					862:865	the fundamental data	846:865	the fundamental data of healing process	846:884	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	9	1	theme	neutral	1687:1693	arg1	polysaccharide					1695:1708	neutral polysaccharide	1687:1708	neutral polysaccharide	1687:1708	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	2	2	theme	skin	328:331	arg1	structure					333:341	the lost or damaged skin structure	308:341	the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it	308:458	Wound enclosure involves many processes to restore the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it.					
32213731	6	3	theme	wound	1213:1217	arg1	healing					1219:1225	wound healing	1213:1225	wound healing	1213:1225	Neutral polysaccharide was more widely distributed compared to the acid polysaccharide in almost all stages of wound healing.					
32213731	3	4	theme	contents	528:535	arg1	collagen					541:548	collagen	541:548	collagen in untreated full-thickness wound healing	541:590	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	3	4	theme	contents	528:535	arg1	alteration					499:508	alteration	499:508	alteration of polysaccharide contents	499:535	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	5	5	from	stages	991:996	arg1	days					1013:1016	about 30-50 days	1001:1016	about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral)	1001:1099	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	1	6	theme	Sunda	171:175	arg1	porcupine					177:185	the Sunda porcupine	167:185	the Sunda porcupine	167:185	Wound healing in the Sunda porcupine is believed to occur quickly, although the wound is large and severe.					
32213731	4	7	theme	healing	870:876	arg1	process					878:884	healing process	870:884	healing process	870:884	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	5	8	dep	days	1013:1016	arg1	days					1080:1083	30-50 days	1074:1083	30-50 days in lumbosacral	1074:1098	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	5	8	dep	days	1013:1016	arg1	days					1048:1051	21-30 days	1042:1051	21-30 days in thoracodorsal	1042:1068	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	3	9	theme	untreated	553:561	arg1	healing					584:590	untreated full-thickness wound healing	553:590	untreated full-thickness wound healing	553:590	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	9	10	dep	collagen	1730:1737	arg1	to					1741:1742	to	1741:1742	to	1741:1742	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	2	11	theme	many	282:285	arg1	processes					287:295	many processes	282:295	many processes	282:295	Wound enclosure involves many processes to restore the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it.					
32213731	5	12	from	days	1080:1083	arg1	lumbosacral					1088:1098	lumbosacral	1088:1098	lumbosacral	1088:1098	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	5	12	from	days	1080:1083	arg1	thoracodorsal					1056:1068	thoracodorsal	1056:1068	thoracodorsal	1056:1068	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	4	13	theme	process	878:884	arg1	data					862:865	the fundamental data	846:865	the fundamental data of healing process	846:884	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	7	14	theme	healing	1344:1350	arg1	process					1352:1358	healing process	1344:1358	healing process	1344:1358	The ratio of collagen I to III appeared to be higher in the thoracodorsal region than the lumbosacral region during healing process.					
32213731	4	15	theme	Schiff	715:720	arg1	pH					735:736	periodic acid Schiff, alcian blue pH 2.5	701:740	pH	735:736	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	4	15	theme	Schiff	715:720	arg1	LV-SEM					820:825	LV-SEM	820:825	LV-SEM	820:825	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	3	16	theme	wound	578:582	arg1	healing					584:590	untreated full-thickness wound healing	553:590	untreated full-thickness wound healing	553:590	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	8	17	theme	granulation	1446:1456	arg1	tissue					1458:1463	the wound border and granulation tissue	1425:1463	tissue	1458:1463	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	4	18	theme	alcian	723:728	arg1	pH					735:736	periodic acid Schiff, alcian blue pH 2.5	701:740	pH	735:736	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	4	18	theme	alcian	723:728	arg1	LV-SEM					820:825	LV-SEM	820:825	LV-SEM	820:825	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	5	19	theme	progressive	942:952	arg1	contraction					960:970	progressive wound contraction	942:970	progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral)	942:1099	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	3	20	theme	lumbosacral	617:627	arg1	regions					629:635	the thoracodorsal and lumbosacral regions	595:635	the thoracodorsal and lumbosacral regions in the Sunda porcupines	595:659	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	9	21	theme	high	1716:1719	arg1	ratio					1721:1725	a high ratio	1714:1725	a high ratio of collagen I to III	1714:1746	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	3	22	from	collagen	541:548	arg1	healing					584:590	untreated full-thickness wound healing	553:590	untreated full-thickness wound healing	553:590	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	5	23	from	days	1048:1051	arg1	lumbosacral					1088:1098	lumbosacral	1088:1098	lumbosacral	1088:1098	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	5	23	from	days	1048:1051	arg1	thoracodorsal					1056:1068	thoracodorsal	1056:1068	thoracodorsal	1056:1068	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	3	24	theme	thoracodorsal	599:611	arg1	regions					629:635	the thoracodorsal and lumbosacral regions	595:635	the thoracodorsal and lumbosacral regions in the Sunda porcupines	595:659	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	1	25	theme	Wound	150:154	arg1	healing					156:162	Wound healing	150:162	Wound healing in the Sunda porcupine	150:185	Wound healing in the Sunda porcupine is believed to occur quickly, although the wound is large and severe.					
32213731	4	26	theme	periodic	701:708	arg1	pH					735:736	periodic acid Schiff, alcian blue pH 2.5	701:740	pH	735:736	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	4	26	theme	periodic	701:708	arg1	LV-SEM					820:825	LV-SEM	820:825	LV-SEM	820:825	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	0	27	theme	wound	93:97	arg1	healing					99:105	cutaneous wound healing	83:105	cutaneous wound healing in the Sunda porcupine (Hystrix javanica)	83:147	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	1	28	from	healing	156:162	arg1	porcupine					177:185	the Sunda porcupine	167:185	the Sunda porcupine	167:185	Wound healing in the Sunda porcupine is believed to occur quickly, although the wound is large and severe.					
32213731	5	29	theme	Wound	887:891	arg1	healing					893:899	Wound healing	887:899	Wound healing	887:899	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	4	30	theme	red	755:757	arg1	method					768:773	picrosirius red staining method	743:773	picrosirius red staining method	743:773	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	9	31	theme	wound	1564:1568	arg1	healing					1570:1576	cutaneous full thickness wound healing	1539:1576	cutaneous full thickness wound healing in the Sunda porcupine	1539:1599	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	8	32	theme	thin	1502:1505	arg1	fiber					1517:1521	thin and thick fiber	1502:1521	thin and thick fiber	1502:1521	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	4	33	theme	method	768:773	arg1	imaging					828:834	periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging	701:834	periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging	701:834	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	0	34	theme	Sunda	114:118	arg1	javanica					139:146	Hystrix javanica	131:146	Hystrix javanica	131:146	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	0	34	theme	Sunda	114:118	arg1	porcupine					120:128	the Sunda porcupine	110:128	the Sunda porcupine (Hystrix javanica)	110:147	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	5	35	theme	overlapping	979:989	arg1	stages					991:996	4 overlapping stages	977:996	4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral)	977:1099	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	8	36	theme	thick	1511:1515	arg1	fiber					1517:1521	thin and thick fiber	1502:1521	thin and thick fiber	1502:1521	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	4	37	theme	pH	735:736	arg1	imaging					828:834	periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging	701:834	periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging	701:834	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	0	38	theme	Morphological	0:12	arg1	evaluation					14:23	Morphological evaluation	0:23	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).	0:148	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	9	39	theme	full	1549:1552	arg1	healing					1570:1576	cutaneous full thickness wound healing	1539:1576	cutaneous full thickness wound healing in the Sunda porcupine	1539:1599	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	7	40	theme	thoracodorsal	1288:1300	arg1	region					1302:1307	the thoracodorsal region	1284:1307	the thoracodorsal region	1284:1307	The ratio of collagen I to III appeared to be higher in the thoracodorsal region than the lumbosacral region during healing process.					
32213731	8	41	theme	LV-SEM	1361:1366	arg1	imaging					1368:1374	LV-SEM imaging	1361:1374	LV-SEM imaging	1361:1374	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	5	42	theme	wound	954:958	arg1	contraction					960:970	progressive wound contraction	942:970	progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral)	942:1099	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	9	43	from	healing	1570:1576	arg1	porcupine					1591:1599	the Sunda porcupine	1581:1599	the Sunda porcupine	1581:1599	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	3	44	theme	Sunda	644:648	arg1	porcupines					650:659	the Sunda porcupines	640:659	the Sunda porcupines	640:659	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	4	45	theme	Microscope	808:817	arg1	imaging					828:834	periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging	701:834	periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging	701:834	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	0	46	theme	content	43:49	arg1	evaluation					14:23	Morphological evaluation	0:23	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).	0:148	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	2	47	theme	damaged	320:326	arg1	structure					333:341	the lost or damaged skin structure	308:341	the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it	308:458	Wound enclosure involves many processes to restore the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it.					
32213731	0	48	theme	collagen	55:62	arg1	composition					64:74	collagen composition	55:74	collagen composition	55:74	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	5	49	theme	wound	1028:1032	arg1	closed					1034:1039	the wound closed	1024:1039	the wound closed	1024:1039	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	8	50	theme	tissue	1405:1410	arg1	structure					1412:1420	connective tissue structure	1394:1420	connective tissue structure	1394:1420	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	2	51	theme	lost	312:315	arg1	structure					333:341	the lost or damaged skin structure	308:341	the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it	308:458	Wound enclosure involves many processes to restore the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it.					
32213731	4	52	theme	Scanning	790:797	arg1	Microscope					808:817	Low Vacuum Scanning Electron Microscope	779:817	Low Vacuum Scanning Electron Microscope	779:817	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	7	53	theme	collagen	1241:1248	arg1	higher					1274:1279	higher	1274:1279	higher	1274:1279	The ratio of collagen I to III appeared to be higher in the thoracodorsal region than the lumbosacral region during healing process.					
32213731	7	53	theme	collagen	1241:1248	arg1	ratio					1232:1236	The ratio	1228:1236	The ratio of collagen I to III	1228:1257	The ratio of collagen I to III appeared to be higher in the thoracodorsal region than the lumbosacral region during healing process.					
32213731	3	54	theme	study	477:481	arg1	aim					465:467	The aim	461:467	The aim of this study	461:481	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	9	55	theme	thoracodorsal	1624:1636	arg1	region					1638:1643	the thoracodorsal region	1620:1643	the thoracodorsal region	1620:1643	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	3	56	from	regions	629:635	arg1	porcupines					650:659	the Sunda porcupines	640:659	the Sunda porcupines	640:659	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	3	57	theme	polysaccharide	513:526	arg1	contents					528:535	polysaccharide contents	513:535	polysaccharide contents	513:535	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	6	58	theme	acid	1169:1172	arg1	polysaccharide					1174:1187	the acid polysaccharide	1165:1187	the acid polysaccharide	1165:1187	Neutral polysaccharide was more widely distributed compared to the acid polysaccharide in almost all stages of wound healing.					
32213731	2	59	theme	Wound	257:261	arg1	enclosure					263:271	Wound enclosure	257:271	Wound enclosure	257:271	Wound enclosure involves many processes to restore the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it.					
32213731	8	60	from	changes	1383:1389	arg1	border					1435:1440	the wound border and granulation tissue	1425:1463	border	1435:1440	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	8	60	from	changes	1383:1389	arg1	tissue					1458:1463	the wound border and granulation tissue	1425:1463	tissue	1458:1463	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	8	60	from	changes	1383:1389	arg1	structure					1412:1420	connective tissue structure	1394:1420	connective tissue structure	1394:1420	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	4	61	theme	Histological	662:673	arg1	analysis					675:682	Histological analysis	662:682	Histological analysis	662:682	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	0	62	theme	Hystrix	131:137	arg1	javanica					139:146	Hystrix javanica	131:146	Hystrix javanica	131:146	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	0	62	theme	Hystrix	131:137	arg1	porcupine					120:128	the Sunda porcupine	110:128	the Sunda porcupine (Hystrix javanica)	110:147	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	9	63	theme	Sunda	1585:1589	arg1	porcupine					1591:1599	the Sunda porcupine	1581:1599	the Sunda porcupine	1581:1599	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	0	64	from	healing	99:105	arg1	javanica					139:146	Hystrix javanica	131:146	Hystrix javanica	131:146	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	0	64	from	healing	99:105	arg1	porcupine					120:128	the Sunda porcupine	110:128	the Sunda porcupine (Hystrix javanica)	110:147	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	7	65	dep	collagen	1241:1248	arg1	to					1252:1253	to	1252:1253	to	1252:1253	The ratio of collagen I to III appeared to be higher in the thoracodorsal region than the lumbosacral region during healing process.					
32213731	6	66	theme	healing	1219:1225	arg1	stages					1203:1208	almost all stages	1192:1208	almost all stages of wound healing	1192:1225	Neutral polysaccharide was more widely distributed compared to the acid polysaccharide in almost all stages of wound healing.					
32213731	5	67	with	contraction	960:970	arg1	stages					991:996	4 overlapping stages	977:996	4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral)	977:1099	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	3	68	theme	full-thickness	563:576	arg1	healing					584:590	untreated full-thickness wound healing	553:590	untreated full-thickness wound healing	553:590	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	9	69	theme	polysaccharide	1695:1708	arg1	role					1679:1682	the role	1675:1682	the role of neutral polysaccharide	1675:1708	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	9	69	theme	polysaccharide	1695:1708	arg1	ratio					1721:1725	a high ratio	1714:1725	a high ratio of collagen I to III	1714:1746	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	4	70	theme	acid	710:713	arg1	pH					735:736	periodic acid Schiff, alcian blue pH 2.5	701:740	pH	735:736	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	4	70	theme	acid	710:713	arg1	LV-SEM					820:825	LV-SEM	820:825	LV-SEM	820:825	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	4	71	theme	Low	779:781	arg1	Microscope					808:817	Low Vacuum Scanning Electron Microscope	779:817	Low Vacuum Scanning Electron Microscope	779:817	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	6	72	theme	all	1199:1201	arg1	stages					1203:1208	almost all stages	1192:1208	almost all stages of wound healing	1192:1225	Neutral polysaccharide was more widely distributed compared to the acid polysaccharide in almost all stages of wound healing.					
32213731	0	73	theme	cutaneous	83:91	arg1	healing					99:105	cutaneous wound healing	83:105	cutaneous wound healing in the Sunda porcupine (Hystrix javanica)	83:147	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	7	74	theme	lumbosacral	1318:1328	arg1	region					1330:1335	the lumbosacral region	1314:1335	the lumbosacral region during healing process	1314:1358	The ratio of collagen I to III appeared to be higher in the thoracodorsal region than the lumbosacral region during healing process.					
32213731	3	75	from	alteration	499:508	arg1	healing					584:590	untreated full-thickness wound healing	553:590	untreated full-thickness wound healing	553:590	The aim of this study was to evaluate alteration of polysaccharide contents and collagen in untreated full-thickness wound healing in the thoracodorsal and lumbosacral regions in the Sunda porcupines.					
32213731	9	76	theme	collagen	1730:1737	arg1	role					1679:1682	the role	1675:1682	the role of neutral polysaccharide	1675:1708	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	9	76	theme	collagen	1730:1737	arg1	ratio					1721:1725	a high ratio	1714:1725	a high ratio of collagen I to III	1714:1746	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	8	77	theme	wound	1429:1433	arg1	border					1435:1440	the wound border and granulation tissue	1425:1463	border	1435:1440	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	4	78	theme	picrosirius	743:753	arg1	method					768:773	picrosirius red staining method	743:773	picrosirius red staining method	743:773	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	8	79	theme	fiber	1517:1521	arg1	abundant					1480:1487	abundant	1480:1487	abundant	1480:1487	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	5	80	dep	began	901:905	arg1	followed					930:937	followed	930:937	followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral)	930:1099	Wound healing began with re-epithelization followed by progressive wound contraction with 4 overlapping stages in about 30-50 days until the wound closed (21-30 days in thoracodorsal and 30-50 days in lumbosacral).					
32213731	9	81	theme	cutaneous	1539:1547	arg1	healing					1570:1576	cutaneous full thickness wound healing	1539:1576	cutaneous full thickness wound healing in the Sunda porcupine	1539:1599	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	2	82	theme	wound	433:437	arg1	tissue					439:444	wound tissue	433:444	wound tissue	433:444	Wound enclosure involves many processes to restore the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it.					
32213731	4	83	theme	blue	730:733	arg1	pH					735:736	periodic acid Schiff, alcian blue pH 2.5	701:740	pH	735:736	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	4	83	theme	blue	730:733	arg1	LV-SEM					820:825	LV-SEM	820:825	LV-SEM	820:825	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	0	84	theme	polysaccharide	28:41	arg1	content					43:49	polysaccharide content	28:49	polysaccharide content	28:49	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	9	85	theme	thickness	1554:1562	arg1	healing					1570:1576	cutaneous full thickness wound healing	1539:1576	cutaneous full thickness wound healing in the Sunda porcupine	1539:1599	In conclusion, cutaneous full thickness wound healing in the Sunda porcupine occurred faster in the thoracodorsal region, which might be correlated to the role of neutral polysaccharide and a high ratio of collagen I to III.					
32213731	2	86	theme	main	404:407	arg1	collagen					387:394	collagen	387:394	collagen	387:394	Wound enclosure involves many processes to restore the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it.					
32213731	2	86	theme	main	404:407	arg1	polysaccharide-protein					360:381	polysaccharide-protein	360:381	polysaccharide-protein	360:381	Wound enclosure involves many processes to restore the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it.					
32213731	2	86	theme	main	404:407	arg1	components					409:418	the main components	400:418	the main components deposited in wound tissue to restore it	400:458	Wound enclosure involves many processes to restore the lost or damaged skin structure where conjugated polysaccharide-protein and collagen, as the main components deposited in wound tissue to restore it.					
32213731	4	87	theme	Electron	799:806	arg1	Microscope					808:817	Low Vacuum Scanning Electron Microscope	779:817	Low Vacuum Scanning Electron Microscope	779:817	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	0	88	theme	composition	64:74	arg1	evaluation					14:23	Morphological evaluation	0:23	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).	0:148	Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica).					
32213731	6	89	theme	Neutral	1102:1108	arg1	polysaccharide					1110:1123	Neutral polysaccharide	1102:1123	Neutral polysaccharide	1102:1123	Neutral polysaccharide was more widely distributed compared to the acid polysaccharide in almost all stages of wound healing.					
32213731	4	90	theme	staining	759:766	arg1	method					768:773	picrosirius red staining method	743:773	picrosirius red staining method	743:773	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32213731	8	91	theme	connective	1394:1403	arg1	structure					1412:1420	connective tissue structure	1394:1420	connective tissue structure	1394:1420	LV-SEM imaging showed changes in connective tissue structure in the wound border and granulation tissue which appeared abundant and mixed of thin and thick fiber.					
32213731	4	92	theme	Vacuum	783:788	arg1	Microscope					808:817	Low Vacuum Scanning Electron Microscope	779:817	Low Vacuum Scanning Electron Microscope	779:817	Histological analysis was performed by periodic acid Schiff, alcian blue pH 2.5, picrosirius red staining method and Low Vacuum Scanning Electron Microscope (LV-SEM) imaging to obtain the fundamental data of healing process.					
32330800	5	0	theme	environment	1282:1292	arg1	protection					1264:1273	protection	1264:1273	protection of the environment and human health	1264:1309	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	2	1	theme	chemical	418:425	arg1	functionalities					427:441	chemical functionalities	418:441	chemical functionalities	418:441	The three-dimensional (3D) graphene composite demonstrated relatively high temperature stability, chemical functionalities and porous sponge-like structure.					
32330800	1	2	theme	effective	284:292	arg1	technique					309:317	facile, eco-friendly and cost effective ice-templating technique	254:317	facile, eco-friendly and cost effective ice-templating technique	254:317	Xanthan integrated graphene oxide functionalized by titanium dioxide was successfully prepared through facile, eco-friendly and cost effective ice-templating technique.					
32330800	0	3	theme	dioxide	90:96	arg1	composite					98:106	3D hierarchical graphene oxide/titanium dioxide composite	50:106	3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater	50:148	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	4	4	theme	initial	973:979	arg1	capacity					992:999	its initial adsorption capacity	969:999	its initial adsorption capacity	969:999	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	1	5	theme	ice-templating	294:307	arg1	technique					309:317	facile, eco-friendly and cost effective ice-templating technique	254:317	facile, eco-friendly and cost effective ice-templating technique	254:317	Xanthan integrated graphene oxide functionalized by titanium dioxide was successfully prepared through facile, eco-friendly and cost effective ice-templating technique.					
32330800	0	6	theme	adsorptive	112:121	arg1	removal					128:134	adsorptive lead removal	112:134	adsorptive lead removal in wastewater	112:148	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	5	7	theme	human	1298:1302	arg1	health					1304:1309	human health	1298:1309	human health	1298:1309	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	4	8	theme	adsorption	981:990	arg1	capacity					992:999	its initial adsorption capacity	969:999	its initial adsorption capacity	969:999	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	5	9	theme	3D	1170:1171	arg1	composite					1182:1190	the as-prepared 3D graphene composite	1154:1190	the as-prepared 3D graphene composite	1154:1190	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	3	10	theme	kinetic	620:626	arg1	correlations					676:687	kinetic adhered to the Langmuir and pseudo-second-order correlations	620:687	kinetic adhered to the Langmuir and pseudo-second-order correlations	620:687	The adsorption of lead was favored by high initial concentration and shaking speed at the operational solution pH. The process equilibrium and kinetic adhered to the Langmuir and pseudo-second-order correlations, respectively.					
32330800	5	11	theme	environmentally	1124:1138	arg1	friendliness					1140:1151	environmentally friendliness	1124:1151	environmentally friendliness	1124:1151	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	11	theme	environmentally	1124:1138	arg1	capacities					1085:1094	comparatively high lead adsorption capacities	1050:1094	comparatively high lead adsorption capacities	1050:1094	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	4	12	theme	mild	874:877	arg1	conditions					879:888	mild conditions	874:888	mild conditions	874:888	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	3	13	theme	adhered	628:634	arg1	correlations					676:687	kinetic adhered to the Langmuir and pseudo-second-order correlations	620:687	kinetic adhered to the Langmuir and pseudo-second-order correlations	620:687	The adsorption of lead was favored by high initial concentration and shaking speed at the operational solution pH. The process equilibrium and kinetic adhered to the Langmuir and pseudo-second-order correlations, respectively.					
32330800	4	14	used	used	927:930	arg2	it					842:843	it	842:843	it	842:843	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	5	15	from	potential	1213:1221	arg1	separation					1249:1258	heavy metal-wastewater separation	1226:1258	heavy metal-wastewater separation	1226:1258	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	4	16	theme	graphene	727:734	arg1	composite					736:744	The biomass integrated graphene composite	704:744	The biomass integrated graphene composite	704:744	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	2	17	theme	three-dimensional	324:340	arg1	composite					356:364	The three-dimensional (3D) graphene composite	320:364	The three-dimensional (3D) graphene composite	320:364	The three-dimensional (3D) graphene composite demonstrated relatively high temperature stability, chemical functionalities and porous sponge-like structure.					
32330800	4	18	dep	acid	910:913	arg1	30 °C					916:920	30 °C	916:920	30 °C	916:920	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	4	19	theme	adsorption-regeneration	1014:1036	arg1	cycle					1038:1042	the fifth adsorption-regeneration cycle	1004:1042	the fifth adsorption-regeneration cycle	1004:1042	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	0	20	theme	lead	123:126	arg1	removal					128:134	adsorptive lead removal	112:134	adsorptive lead removal in wastewater	112:148	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	5	21	theme	adequate	1097:1104	arg1	capacities					1085:1094	comparatively high lead adsorption capacities	1050:1094	comparatively high lead adsorption capacities	1050:1094	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	21	theme	adequate	1097:1104	arg1	recyclability					1106:1118	adequate recyclability	1097:1118	adequate recyclability	1097:1118	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	1	22	theme	titanium	203:210	arg1	dioxide					212:218	titanium dioxide	203:218	titanium dioxide	203:218	Xanthan integrated graphene oxide functionalized by titanium dioxide was successfully prepared through facile, eco-friendly and cost effective ice-templating technique.					
32330800	5	23	dep	environment	1282:1292	arg1	the					1278:1280	the	1278:1280	the	1278:1280	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	4	24	theme	adsorption	761:770	arg1	capacities					772:781	maximum adsorption capacities	753:781	maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C.	753:830	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	5	25	theme	lead	1069:1072	arg1	friendliness					1140:1151	environmentally friendliness	1124:1151	environmentally friendliness	1124:1151	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	25	theme	lead	1069:1072	arg1	capacities					1085:1094	comparatively high lead adsorption capacities	1050:1094	comparatively high lead adsorption capacities	1050:1094	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	25	theme	lead	1069:1072	arg1	recyclability					1106:1118	adequate recyclability	1097:1118	adequate recyclability	1097:1118	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	2	26	theme	sponge-like	454:464	arg1	structure					466:474	porous sponge-like structure	447:474	porous sponge-like structure	447:474	The three-dimensional (3D) graphene composite demonstrated relatively high temperature stability, chemical functionalities and porous sponge-like structure.					
32330800	0	27	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of xanthan biopolymer	0:37	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	5	28	theme	high	1064:1067	arg1	friendliness					1140:1151	environmentally friendliness	1124:1151	environmentally friendliness	1124:1151	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	28	theme	high	1064:1067	arg1	capacities					1085:1094	comparatively high lead adsorption capacities	1050:1094	comparatively high lead adsorption capacities	1050:1094	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	28	theme	high	1064:1067	arg1	recyclability					1106:1118	adequate recyclability	1097:1118	adequate recyclability	1097:1118	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	2	29	theme	porous	447:452	arg1	structure					466:474	porous sponge-like structure	447:474	porous sponge-like structure	447:474	The three-dimensional (3D) graphene composite demonstrated relatively high temperature stability, chemical functionalities and porous sponge-like structure.					
32330800	0	30	theme	xanthan	20:26	arg1	biopolymer					28:37	xanthan biopolymer	20:37	xanthan biopolymer	20:37	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	1	31	dep	integrated	159:168	arg1	Xanthan					151:157	Xanthan	151:157	Xanthan	151:157	Xanthan integrated graphene oxide functionalized by titanium dioxide was successfully prepared through facile, eco-friendly and cost effective ice-templating technique.					
32330800	4	32	dep	regenerable	856:866	arg1	acid					910:913	0.1 M hydrochloric acid	891:913	0.1 M hydrochloric acid	891:913	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	4	33	theme	0.1 M	891:895	arg1	acid					910:913	0.1 M hydrochloric acid	891:913	0.1 M hydrochloric acid	891:913	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	5	34	theme	adsorption	1074:1083	arg1	friendliness					1140:1151	environmentally friendliness	1124:1151	environmentally friendliness	1124:1151	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	34	theme	adsorption	1074:1083	arg1	capacities					1085:1094	comparatively high lead adsorption capacities	1050:1094	comparatively high lead adsorption capacities	1050:1094	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	34	theme	adsorption	1074:1083	arg1	recyclability					1106:1118	adequate recyclability	1097:1118	adequate recyclability	1097:1118	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	35	contain	has	1192:1194	arg1	composite					1182:1190	the as-prepared 3D graphene composite	1154:1190	the as-prepared 3D graphene composite	1154:1190	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	35	contain	has	1192:1194	arg2	potential					1213:1221	high application potential	1196:1221	high application potential in heavy metal-wastewater separation for protection of the environment and human health	1196:1309	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	2	36	dep	three-dimensional	324:340	arg1	3D					343:344	3D	343:344	3D	343:344	The three-dimensional (3D) graphene composite demonstrated relatively high temperature stability, chemical functionalities and porous sponge-like structure.					
32330800	3	37	theme	operational	567:577	arg1	solution					579:586	the operational solution	563:586	the operational solution pH. The process equilibrium and kinetic adhered to the Langmuir and pseudo-second-order correlations, respectively	563:701	The adsorption of lead was favored by high initial concentration and shaking speed at the operational solution pH. The process equilibrium and kinetic adhered to the Langmuir and pseudo-second-order correlations, respectively.					
32330800	4	38	theme	maximum	753:759	arg1	capacities					772:781	maximum adsorption capacities	753:781	maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C.	753:830	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	3	39	theme	initial	520:526	arg1	concentration					528:540	high initial concentration	515:540	high initial concentration	515:540	The adsorption of lead was favored by high initial concentration and shaking speed at the operational solution pH. The process equilibrium and kinetic adhered to the Langmuir and pseudo-second-order correlations, respectively.					
32330800	0	40	theme	biopolymer	28:37	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of xanthan biopolymer	0:37	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	5	41	theme	health	1304:1309	arg1	protection					1264:1273	protection	1264:1273	protection of the environment and human health	1264:1309	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	3	42	theme	lead	495:498	arg1	adsorption					481:490	The adsorption	477:490	The adsorption of lead	477:498	The adsorption of lead was favored by high initial concentration and shaking speed at the operational solution pH. The process equilibrium and kinetic adhered to the Langmuir and pseudo-second-order correlations, respectively.					
32330800	5	43	theme	heavy	1226:1230	arg1	separation					1249:1258	heavy metal-wastewater separation	1226:1258	heavy metal-wastewater separation	1226:1258	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	5	44	theme	as-prepared	1158:1168	arg1	composite					1182:1190	the as-prepared 3D graphene composite	1154:1190	the as-prepared 3D graphene composite	1154:1190	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	1	45	theme	facile	254:259	arg1	technique					309:317	facile, eco-friendly and cost effective ice-templating technique	254:317	facile, eco-friendly and cost effective ice-templating technique	254:317	Xanthan integrated graphene oxide functionalized by titanium dioxide was successfully prepared through facile, eco-friendly and cost effective ice-templating technique.					
32330800	0	46	theme	hierarchical	53:64	arg1	composite					98:106	3D hierarchical graphene oxide/titanium dioxide composite	50:106	3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater	50:148	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	5	47	theme	metal-wastewater	1232:1247	arg1	separation					1249:1258	heavy metal-wastewater separation	1226:1258	heavy metal-wastewater separation	1226:1258	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	3	48	theme	pseudo-second-order	656:674	arg1	correlations					676:687	kinetic adhered to the Langmuir and pseudo-second-order correlations	620:687	kinetic adhered to the Langmuir and pseudo-second-order correlations	620:687	The adsorption of lead was favored by high initial concentration and shaking speed at the operational solution pH. The process equilibrium and kinetic adhered to the Langmuir and pseudo-second-order correlations, respectively.					
32330800	3	49	dep	equilibrium	604:614	arg1	pH.					588:590	pH.	588:590	pH.	588:590	The adsorption of lead was favored by high initial concentration and shaking speed at the operational solution pH. The process equilibrium and kinetic adhered to the Langmuir and pseudo-second-order correlations, respectively.					
32330800	0	50	theme	3D	50:51	arg1	composite					98:106	3D hierarchical graphene oxide/titanium dioxide composite	50:106	3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater	50:148	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	4	51	theme	capacity	992:999	arg1	capacity					992:999	its initial adsorption capacity	969:999	its initial adsorption capacity	969:999	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	4	51	theme	capacity	992:999	arg1	%					964:964	84.78%	959:964	84.78% of its initial adsorption capacity	959:999	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	0	52	from	removal	128:134	arg1	wastewater					139:148	wastewater	139:148	wastewater	139:148	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	5	53	theme	graphene	1173:1180	arg1	composite					1182:1190	the as-prepared 3D graphene composite	1154:1190	the as-prepared 3D graphene composite	1154:1190	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	2	54	theme	temperature	395:405	arg1	stability					407:415	relatively high temperature stability	379:415	relatively high temperature stability	379:415	The three-dimensional (3D) graphene composite demonstrated relatively high temperature stability, chemical functionalities and porous sponge-like structure.					
32330800	4	55	theme	fifth	1008:1012	arg1	cycle					1038:1042	the fifth adsorption-regeneration cycle	1004:1042	the fifth adsorption-regeneration cycle	1004:1042	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	2	56	theme	high	390:393	arg1	stability					407:415	relatively high temperature stability	379:415	relatively high temperature stability	379:415	The three-dimensional (3D) graphene composite demonstrated relatively high temperature stability, chemical functionalities and porous sponge-like structure.					
32330800	2	57	theme	graphene	347:354	arg1	composite					356:364	The three-dimensional (3D) graphene composite	320:364	The three-dimensional (3D) graphene composite	320:364	The three-dimensional (3D) graphene composite demonstrated relatively high temperature stability, chemical functionalities and porous sponge-like structure.					
32330800	5	58	theme	high	1196:1199	arg1	potential					1213:1221	high application potential	1196:1221	high application potential in heavy metal-wastewater separation for protection of the environment and human health	1196:1309	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	4	59	theme	hydrochloric	897:908	arg1	acid					910:913	0.1 M hydrochloric acid	891:913	0.1 M hydrochloric acid	891:913	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	3	60	theme	process	596:602	arg1	equilibrium					604:614	The process equilibrium	592:614	The process equilibrium	592:614	The adsorption of lead was favored by high initial concentration and shaking speed at the operational solution pH. The process equilibrium and kinetic adhered to the Langmuir and pseudo-second-order correlations, respectively.					
32330800	1	61	theme	eco-friendly	262:273	arg1	technique					309:317	facile, eco-friendly and cost effective ice-templating technique	254:317	facile, eco-friendly and cost effective ice-templating technique	254:317	Xanthan integrated graphene oxide functionalized by titanium dioxide was successfully prepared through facile, eco-friendly and cost effective ice-templating technique.					
32330800	0	62	theme	oxide/titanium	75:88	arg1	composite					98:106	3D hierarchical graphene oxide/titanium dioxide composite	50:106	3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater	50:148	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	5	63	theme	application	1201:1211	arg1	potential					1213:1221	high application potential	1196:1221	high application potential in heavy metal-wastewater separation for protection of the environment and human health	1196:1309	With comparatively high lead adsorption capacities, adequate recyclability and environmentally friendliness, the as-prepared 3D graphene composite has high application potential in heavy metal-wastewater separation for protection of the environment and human health.					
32330800	1	64	theme	integrated	159:168	arg1	oxide					179:183	Xanthan integrated graphene oxide	151:183	Xanthan integrated graphene oxide functionalized by titanium dioxide	151:218	Xanthan integrated graphene oxide functionalized by titanium dioxide was successfully prepared through facile, eco-friendly and cost effective ice-templating technique.					
32330800	3	65	theme	high	515:518	arg1	concentration					528:540	high initial concentration	515:540	high initial concentration	515:540	The adsorption of lead was favored by high initial concentration and shaking speed at the operational solution pH. The process equilibrium and kinetic adhered to the Langmuir and pseudo-second-order correlations, respectively.					
32330800	4	66	theme	integrated	716:725	arg1	composite					736:744	The biomass integrated graphene composite	704:744	The biomass integrated graphene composite	704:744	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
32330800	0	67	theme	graphene	66:73	arg1	composite					98:106	3D hierarchical graphene oxide/titanium dioxide composite	50:106	3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater	50:148	Facile synthesis of xanthan biopolymer integrated 3D hierarchical graphene oxide/titanium dioxide composite for adsorptive lead removal in wastewater.					
32330800	1	68	theme	graphene	170:177	arg1	oxide					179:183	Xanthan integrated graphene oxide	151:183	Xanthan integrated graphene oxide functionalized by titanium dioxide	151:218	Xanthan integrated graphene oxide functionalized by titanium dioxide was successfully prepared through facile, eco-friendly and cost effective ice-templating technique.					
32330800	4	69	theme	biomass	708:714	arg1	composite					736:744	The biomass integrated graphene composite	704:744	The biomass integrated graphene composite	704:744	The biomass integrated graphene composite showed maximum adsorption capacities ranging from 132.18 to 199.22 mg/g for 30-70 °C. Moreover, it was highly regenerable under mild conditions (0.1 M hydrochloric acid, 30 °C) and used repeatedly while retaining 84.78% of its initial adsorption capacity at the fifth adsorption-regeneration cycle.					
33688734	2	0	theme	polymerization	396:409	arg1	degree					386:391	their degree	380:391	their degree of polymerization (DP)	380:414	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	2	0	theme	polymerization	396:409	arg1	position					424:431	the position	420:431	the position of the acetyl groups along the glycan chain	420:475	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	2	1	from	particular	369:378	arg1	dependent					324:332	dependent	324:332	dependent	324:332	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	8	2	theme	oligosaccharides	1550:1565	arg1	release					1539:1545	the release	1535:1545	the release of oligosaccharides from the lobster shell	1535:1588	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	7	3	theme	chitosan	1389:1396	arg1	substrates					1398:1407	partially acetylated chitosan substrates	1368:1407	partially acetylated chitosan substrates	1368:1407	It exhibits a kcat of 67.2 s-1 on partially acetylated chitosan substrates.					
33688734	4	4	theme	reducing	875:882	arg1	end					884:886	their reducing end	869:886	their reducing end	869:886	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	6	5	theme	shelf	1210:1214	arg1	life					1216:1219	its long shelf life	1201:1219	its long shelf life	1201:1219	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	4	6	from	pabuli	716:721	arg1	chitinase					687:695	a newly discovered chitinase	668:695	a newly discovered chitinase from Paenibacillus pabuli (PpChi)	668:729	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	4	7	theme	discovered	676:685	arg1	chitinase					687:695	a newly discovered chitinase	668:695	a newly discovered chitinase from Paenibacillus pabuli (PpChi)	668:729	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	8	8	from	increase	1523:1530	arg1	release					1539:1545	the release	1535:1545	the release of oligosaccharides from the lobster shell	1535:1588	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	8	8	from	increase	1523:1530	arg1	shell					1584:1588	the lobster shell	1572:1588	the lobster shell	1572:1588	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	3	9	theme	structure/bioactivity	588:608	arg1	relationship					610:621	the structure/bioactivity relationship	584:621	the structure/bioactivity relationship of these oligosaccharides	584:647	The production of structurally defined paCOSs in a purified form is highly desirable to better understand the structure/bioactivity relationship of these oligosaccharides.					
33688734	1	10	theme	bioactive	227:235	arg1	chito-oligosaccharides					191:212	Partially acetylated chito-oligosaccharides	170:212	Partially acetylated chito-oligosaccharides (paCOSs)	170:221	Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications.					
33688734	1	10	theme	bioactive	227:235	arg1	compounds					237:245	bioactive compounds	227:245	bioactive compounds with potential medical applications	227:281	Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications.					
33688734	9	11	theme	attractive	1624:1633	arg1	biocatalyst					1635:1645	an attractive biocatalyst	1621:1645	an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure	1621:1726	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
33688734	5	12	dep	as	996:997	arg1	in					999:1000	in	999:1000	in	999:1000	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	6	13	theme	temperature	1246:1256	arg1	range					1258:1262	a large temperature range	1238:1262	a large temperature range	1238:1262	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	5	14	theme	subsite	1028:1034	arg1	specificity					1036:1046	a subsite specificity	1026:1046	a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates	1026:1147	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	3	15	theme	oligosaccharides	632:647	arg1	relationship					610:621	the structure/bioactivity relationship	584:621	the structure/bioactivity relationship of these oligosaccharides	584:647	The production of structurally defined paCOSs in a purified form is highly desirable to better understand the structure/bioactivity relationship of these oligosaccharides.					
33688734	2	16	from	dependent	324:332	arg1	particular					369:378	particular	369:378	particular	369:378	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	9	17	theme	green	1655:1659	arg1	production					1661:1670	the green production	1651:1670	the green production of highly valuable paCOSs with a well-defined structure	1651:1726	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
33688734	9	18	with	paCOSs	1691:1696	arg1	structure					1718:1726	a well-defined structure	1703:1726	a well-defined structure	1703:1726	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
33688734	0	19	from	End	104:106	arg1	N-Acetylation					72:84	a Single N-Acetylation	63:84	a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli	63:167	Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli.					
33688734	6	20	theme	industrial	1312:1321	arg1	processes					1323:1331	industrial processes	1312:1331	industrial processes	1312:1331	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	7	21	from	kcat	1348:1351	arg1	substrates					1398:1407	partially acetylated chitosan substrates	1368:1407	partially acetylated chitosan substrates	1368:1407	It exhibits a kcat of 67.2 s-1 on partially acetylated chitosan substrates.					
33688734	9	22	theme	valuable	1682:1689	arg1	paCOSs					1691:1696	highly valuable paCOSs	1675:1696	highly valuable paCOSs with a well-defined structure	1675:1726	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
33688734	1	23	theme	acetylated	180:189	arg1	paCOSs					215:220	paCOSs	215:220	paCOSs	215:220	Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications.					
33688734	1	23	theme	acetylated	180:189	arg1	chito-oligosaccharides					191:212	Partially acetylated chito-oligosaccharides	170:212	Partially acetylated chito-oligosaccharides (paCOSs)	170:221	Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications.					
33688734	1	23	theme	acetylated	180:189	arg1	compounds					237:245	bioactive compounds	227:245	bioactive compounds with potential medical applications	227:281	Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications.					
33688734	8	24	from	shell	1584:1588	arg1	increase					1523:1530	a sixfold increase	1513:1530	a sixfold increase in the release of oligosaccharides from the lobster shell	1513:1588	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	8	24	from	shell	1584:1588	arg1	release					1539:1545	the release	1535:1545	the release of oligosaccharides from the lobster shell	1535:1588	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	9	25	theme	well-defined	1705:1716	arg1	structure					1718:1726	a well-defined structure	1703:1726	a well-defined structure	1703:1726	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
33688734	4	26	theme	three	817:821	arg1	DP					811:812	a DP	809:812	a DP of three and four	809:830	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	8	27	with	combination	1433:1443	arg1	oxidase					1504:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	6	28	theme	high	1277:1280	arg1	interest					1282:1289	high interest	1277:1289	high interest	1277:1289	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	6	29	from	addition	1153:1160	arg1	stable					1177:1182	stable	1177:1182	stable	1177:1182	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	6	29	from	addition	1153:1160	arg1	enzyme					1167:1172	the enzyme	1163:1172	the enzyme	1163:1172	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	2	30	theme	glycan	464:469	arg1	chain					471:475	the glycan chain	460:475	the glycan chain	460:475	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	8	31	theme	fungal	1472:1477	arg1	oxidase					1504:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	8	32	theme	activity	1488:1495	arg1	oxidase					1504:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	4	33	theme	single	845:850	arg1	N-acetylation					852:864	a single N-acetylation	843:864	a single N-acetylation	843:864	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	2	34	theme	acetyl	440:445	arg1	groups					447:452	the acetyl groups	436:452	the acetyl groups	436:452	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	0	35	theme	Chito-Oligosaccharides	35:56	arg1	Production					0:9	Production	0:9	Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli.	0:168	Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli.					
33688734	9	36	theme	small	1764:1768	arg1	library					1770:1776	the relatively small library	1749:1776	the relatively small library of chito-oligosaccharides currently available	1749:1822	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
33688734	8	37	theme	AA11	1498:1501	arg1	oxidase					1504:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	3	38	from	production	482:491	arg1	form					538:541	a purified form	527:541	a purified form	527:541	The production of structurally defined paCOSs in a purified form is highly desirable to better understand the structure/bioactivity relationship of these oligosaccharides.					
33688734	5	39	from	positions	1091:1099	arg1	residues					1060:1067	GlcN residues	1055:1067	GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates	1055:1147	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	5	40	theme	chitosan	1129:1136	arg1	substrates					1138:1147	the partially acetylated chitosan substrates	1104:1147	the partially acetylated chitosan substrates	1104:1147	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	4	41	theme	four	827:830	arg1	DP					811:812	a DP	809:812	a DP of three and four	809:830	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	0	42	with	Production	0:9	arg1	N-Acetylation					72:84	a Single N-Acetylation	63:84	a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli	63:167	Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli.					
33688734	3	43	theme	defined	509:515	arg1	paCOSs					517:522	structurally defined paCOSs	496:522	structurally defined paCOSs	496:522	The production of structurally defined paCOSs in a purified form is highly desirable to better understand the structure/bioactivity relationship of these oligosaccharides.					
33688734	1	44	theme	potential	252:260	arg1	applications					270:281	potential medical applications	252:281	potential medical applications	252:281	Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications.					
33688734	8	45	theme	lobster	1576:1582	arg1	shell					1584:1588	the lobster shell	1572:1588	the lobster shell	1572:1588	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	3	46	theme	purified	529:536	arg1	form					538:541	a purified form	527:541	a purified form	527:541	The production of structurally defined paCOSs in a purified form is highly desirable to better understand the structure/bioactivity relationship of these oligosaccharides.					
33688734	7	47	theme	acetylated	1378:1387	arg1	substrates					1398:1407	partially acetylated chitosan substrates	1368:1407	partially acetylated chitosan substrates	1368:1407	It exhibits a kcat of 67.2 s-1 on partially acetylated chitosan substrates.					
33688734	7	48	theme	s-1	1361:1363	arg1	kcat					1348:1351	a kcat	1346:1351	a kcat of 67.2 s-1 on partially acetylated chitosan substrates	1346:1407	It exhibits a kcat of 67.2 s-1 on partially acetylated chitosan substrates.					
33688734	0	49	from	pabuli	162:167	arg1	Chitinase					133:141	a Newly Discovered Chitinase	114:141	a Newly Discovered Chitinase from Paenibacillus pabuli	114:167	Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli.					
33688734	4	50	theme	Paenibacillus	702:714	arg1	pabuli					716:721	Paenibacillus pabuli	702:721	Paenibacillus pabuli (PpChi)	702:729	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	4	50	theme	Paenibacillus	702:714	arg1	PpChi					724:728	PpChi	724:728	PpChi	724:728	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	6	51	theme	long	1205:1208	arg1	life					1216:1219	its long shelf life	1201:1219	its long shelf life	1201:1219	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	5	52	theme	glucosamine	934:944	arg1	residues					986:993	glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues	934:993	glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues	934:993	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	0	53	theme	Paenibacillus	148:160	arg1	pabuli					162:167	Paenibacillus pabuli	148:167	Paenibacillus pabuli	148:167	Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli.					
33688734	4	54	with	paCOSs	797:802	arg1	DP					811:812	a DP	809:812	a DP of three and four	809:830	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	4	55	theme	mass	750:753	arg1	spectrometry					755:766	mass spectrometry	750:766	mass spectrometry	750:766	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	6	56	theme	large	1240:1244	arg1	range					1258:1262	a large temperature range	1238:1262	a large temperature range	1238:1262	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	4	57	contain	carry	837:841	arg1	paCOSs					797:802	paCOSs	797:802	paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end	797:886	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	4	57	contain	carry	837:841	arg3	end					884:886	their reducing end	869:886	their reducing end	869:886	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	4	57	contain	carry	837:841	arg2	N-acetylation					852:864	a single N-acetylation	843:864	a single N-acetylation	843:864	Here, we describe a newly discovered chitinase from Paenibacillus pabuli (PpChi) and demonstrate by mass spectrometry that it essentially produces paCOSs with a DP of three and four that carry a single N-acetylation at their reducing end.					
33688734	5	58	theme	GlcN	1055:1058	arg1	residues					1060:1067	GlcN residues	1055:1067	GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates	1055:1147	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	2	59	theme	biological	290:299	arg1	activities					301:310	Their biological activities	284:310	Their biological activities	284:310	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	5	60	theme	specific	910:917	arg1	composition					919:929	this specific composition	905:929	this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1,	905:1014	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	8	61	used	used	1425:1428	arg2	PpChi					1415:1419	PpChi	1415:1419	PpChi	1415:1419	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	0	62	theme	Reducing	95:102	arg1	End					104:106	Their Reducing End	89:106	Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli	89:167	Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli.					
33688734	6	63	from	use	1305:1307	arg1	processes					1323:1331	industrial processes	1312:1331	industrial processes	1312:1331	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	9	64	theme	paCOSs	1691:1696	arg1	production					1661:1670	the green production	1651:1670	the green production of highly valuable paCOSs with a well-defined structure	1651:1726	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
33688734	6	65	from	stable	1177:1182	arg1	addition					1153:1160	addition	1153:1160	addition	1153:1160	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	6	66	theme	potential	1295:1303	arg1	use					1305:1307	potential use	1295:1307	potential use in industrial processes	1295:1331	In addition, the enzyme is stable, as evidenced by its long shelf life, and active over a large temperature range, which is of high interest for potential use in industrial processes.					
33688734	5	67	theme	residues	986:993	arg1	composition					919:929	this specific composition	905:929	this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1,	905:1014	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	2	68	theme	structural	343:352	arg1	properties					354:363	their structural properties	337:363	their structural properties	337:363	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	8	69	theme	discovered	1461:1470	arg1	oxidase					1504:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	5	70	theme	N-acetylglucosamine	957:975	arg1	residues					986:993	glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues	934:993	glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues	934:993	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	8	71	theme	auxilary	1479:1486	arg1	oxidase					1504:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	a recently discovered fungal auxilary activity (AA11) oxidase	1450:1510	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	2	72	dep	particular	369:378	arg1	position					424:431	the position	420:431	the position of the acetyl groups along the glycan chain	420:475	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	2	72	dep	particular	369:378	arg1	degree					386:391	their degree	380:391	their degree of polymerization (DP)	380:414	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	0	73	theme	Defined	27:33	arg1	Chito-Oligosaccharides					35:56	Structurally Defined Chito-Oligosaccharides	14:56	Structurally Defined Chito-Oligosaccharides	14:56	Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli.					
33688734	1	74	with	compounds	237:245	arg1	applications					270:281	potential medical applications	252:281	potential medical applications	252:281	Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications.					
33688734	2	75	theme	groups	447:452	arg1	degree					386:391	their degree	380:391	their degree of polymerization (DP)	380:414	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	2	75	theme	groups	447:452	arg1	position					424:431	the position	420:431	the position of the acetyl groups along the glycan chain	420:475	Their biological activities are largely dependent on their structural properties, in particular their degree of polymerization (DP) and the position of the acetyl groups along the glycan chain.					
33688734	0	76	theme	Single	65:70	arg1	N-Acetylation					72:84	a Single N-Acetylation	63:84	a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli	63:167	Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli.					
33688734	5	77	theme	acetylated	1118:1127	arg1	substrates					1138:1147	the partially acetylated chitosan substrates	1104:1147	the partially acetylated chitosan substrates	1104:1147	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	9	78	theme	library	1770:1776	arg1	expansion					1736:1744	the expansion	1732:1744	the expansion of the relatively small library of chito-oligosaccharides currently available	1732:1822	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
33688734	9	78	theme	library	1770:1776	arg1	biocatalyst					1635:1645	an attractive biocatalyst	1621:1645	an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure	1621:1726	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
33688734	5	79	theme	substrates	1138:1147	arg1	positions					1091:1099	the -2, -3, and -4 positions	1072:1099	the -2, -3, and -4 positions of the partially acetylated chitosan substrates	1072:1147	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	9	80	theme	chito-oligosaccharides	1781:1802	arg1	library					1770:1776	the relatively small library	1749:1776	the relatively small library of chito-oligosaccharides currently available	1749:1822	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
33688734	8	81	theme	sixfold	1515:1521	arg1	increase					1523:1530	a sixfold increase	1513:1530	a sixfold increase in the release of oligosaccharides from the lobster shell	1513:1588	When PpChi was used in combination with a recently discovered fungal auxilary activity (AA11) oxidase, a sixfold increase in the release of oligosaccharides from the lobster shell was measured.					
33688734	3	82	theme	paCOSs	517:522	arg1	production					482:491	The production	478:491	The production of structurally defined paCOSs in a purified form	478:541	The production of structurally defined paCOSs in a purified form is highly desirable to better understand the structure/bioactivity relationship of these oligosaccharides.					
33688734	3	82	theme	paCOSs	517:522	arg1	desirable					553:561	desirable	553:561	desirable	553:561	The production of structurally defined paCOSs in a purified form is highly desirable to better understand the structure/bioactivity relationship of these oligosaccharides.					
33688734	1	83	theme	medical	262:268	arg1	applications					270:281	potential medical applications	252:281	potential medical applications	252:281	Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications.					
33688734	5	84	theme	GlcNAc1	1007:1013	arg1	composition					919:929	this specific composition	905:929	this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1,	905:1014	We propose that this specific composition of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) residues, as in GlcN(GlcNAc1, is due to a subsite specificity toward GlcN residues at the -2, -3, and -4 positions of the partially acetylated chitosan substrates.					
33688734	0	85	theme	Discovered	122:131	arg1	Chitinase					133:141	a Newly Discovered Chitinase	114:141	a Newly Discovered Chitinase from Paenibacillus pabuli	114:167	Production of Structurally Defined Chito-Oligosaccharides with a Single N-Acetylation at Their Reducing End Using a Newly Discovered Chitinase from Paenibacillus pabuli.					
33688734	9	86	theme	available	1814:1822	arg1	chito-oligosaccharides					1781:1802	chito-oligosaccharides	1781:1802	chito-oligosaccharides currently available	1781:1822	PpChi represents an attractive biocatalyst for the green production of highly valuable paCOSs with a well-defined structure and the expansion of the relatively small library of chito-oligosaccharides currently available.					
32446898	0	0	from	isolation	11:19	arg1	algae					62:66	brown algae	56:66	brown algae Turbinaria decurrens	56:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	8	1	theme	regulator	1424:1432	arg1	NF-κB					1434:1438	master regulator NF-κB	1417:1438	master regulator NF-κB	1417:1438	The anti-inflammatory effect of fucoidan was attributed to its capacity on modulating the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines.					
32446898	0	2	dep	in	112:113	arg1	vivo					115:118	vivo	115:118	vivo	115:118	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	8	3	theme	master	1417:1422	arg1	NF-κB					1434:1438	master regulator NF-κB	1417:1438	master regulator NF-κB	1417:1438	The anti-inflammatory effect of fucoidan was attributed to its capacity on modulating the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines.					
32446898	1	4	theme	fucoidan	240:247	arg1	activity					228:235	the anti-nociception and anti-inflammatory activity	185:235	the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model	185:318	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	0	5	theme	Turbinaria	68:77	arg1	algae					62:66	brown algae	56:66	brown algae Turbinaria decurrens	56:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	8	6	theme	antioxidants	1403:1414	arg1	levels					1383:1388	the levels	1379:1388	the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines	1379:1469	The anti-inflammatory effect of fucoidan was attributed to its capacity on modulating the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines.					
32446898	7	7	from	factor	1129:1134	arg1	cytosol					1143:1149	the cytosol	1139:1149	the cytosol	1139:1149	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	2	8	theme	extracted	343:351	arg1	fucoidan					353:360	The extracted fucoidan	339:360	The extracted fucoidan	339:360	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	8	9	theme	enzymatic	1393:1401	arg1	antioxidants					1403:1414	enzymatic antioxidants	1393:1414	enzymatic antioxidants	1393:1414	The anti-inflammatory effect of fucoidan was attributed to its capacity on modulating the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines.					
32446898	5	10	theme	edema	877:881	arg1	condition					883:891	the formalin induced inflammatory edema condition	843:891	the formalin induced inflammatory edema condition	843:891	The fucoidan reduced the licking time thereby suggesting anti-nociceptive effect and decreased the size of paw swelling in the formalin induced inflammatory edema condition.					
32446898	0	11	from	Studies	0:6	arg1	isolation					11:19	isolation	11:19	isolation	11:19	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	11	from	Studies	0:6	arg1	evaluation					93:102	evaluation	93:102	evaluation of it's	93:110	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	11	from	Studies	0:6	arg1	characterization					22:37	characterization	22:37	characterization of fucoidan from brown algae Turbinaria decurrens	22:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	6	12	theme	mice	1053:1056	arg1	activity					1003:1010	the SOD, CAT, GPx, GST and GSH activity	972:1010	the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice	972:1056	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	9	13	theme	reduced	1489:1495	arg1	cytotoxicity					1509:1520	reduced LPS induced cytotoxicity	1489:1520	reduced LPS induced cytotoxicity in IC-21 macrophage	1489:1540	The fucoidan has reduced LPS induced cytotoxicity in IC-21 macrophage at a dose depended on manner.					
32446898	5	14	theme	paw	827:829	arg1	swelling					831:838	paw swelling	827:838	paw swelling	827:838	The fucoidan reduced the licking time thereby suggesting anti-nociceptive effect and decreased the size of paw swelling in the formalin induced inflammatory edema condition.					
32446898	5	15	dep	induced	856:862	arg1	formalin					847:854	formalin	847:854	formalin	847:854	The fucoidan reduced the licking time thereby suggesting anti-nociceptive effect and decreased the size of paw swelling in the formalin induced inflammatory edema condition.					
32446898	6	16	theme	injected	1044:1051	arg1	mice					1053:1056	formalin injected mice	1035:1056	formalin injected mice	1035:1056	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	5	17	theme	induced	856:862	arg1	condition					883:891	the formalin induced inflammatory edema condition	843:891	the formalin induced inflammatory edema condition	843:891	The fucoidan reduced the licking time thereby suggesting anti-nociceptive effect and decreased the size of paw swelling in the formalin induced inflammatory edema condition.					
32446898	3	18	theme	composition	451:461	arg1	analysis					463:470	The monosaccharide composition analysis	432:470	The monosaccharide composition analysis	432:470	The monosaccharide composition analysis revealed that fucoidan encompassed of fucose (59.3%), galactose (12.6%), mannose (9.6%), rhamnose (6.4%) and xylose (11.4%).					
32446898	0	19	theme	it	107:108	arg1	isolation					11:19	isolation	11:19	isolation	11:19	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	19	theme	it	107:108	arg1	evaluation					93:102	evaluation	93:102	evaluation of it's	93:110	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	19	theme	it	107:108	arg1	characterization					22:37	characterization	22:37	characterization of fucoidan from brown algae Turbinaria decurrens	22:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	7	20	theme	expression	1195:1204	arg1	regulation					1172:1181	down regulation	1167:1181	down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice	1167:1290	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	5	21	theme	inflammatory	864:875	arg1	condition					883:891	the formalin induced inflammatory edema condition	843:891	the formalin induced inflammatory edema condition	843:891	The fucoidan reduced the licking time thereby suggesting anti-nociceptive effect and decreased the size of paw swelling in the formalin induced inflammatory edema condition.					
32446898	7	22	from	IL-1β	1244:1248	arg1	mice					1287:1290	fucoidan treated mice	1270:1290	fucoidan treated mice	1270:1290	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	2	23	theme	sugar	386:390	arg1	sulfate					403:409	sulfate	403:409	sulfate	403:409	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	23	theme	sugar	386:390	arg1	sugar					386:390	total sugar	380:390	total sugar	380:390	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	23	theme	sugar	386:390	arg1	%					418:418	3.4%	415:418	3.4% of protein	415:429	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	23	theme	sugar	386:390	arg1	%					398:398	23.51%	393:398	23.51% of sulfate	393:409	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	23	theme	sugar	386:390	arg1	protein					423:429	protein	423:429	protein	423:429	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	23	theme	sugar	386:390	arg1	%					375:375	54.86%	370:375	54.86% of total sugar	370:390	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	24	contain	contain	362:368	arg2	protein					423:429	protein	423:429	protein	423:429	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	24	contain	contain	362:368	arg2	%					398:398	23.51%	393:398	23.51% of sulfate	393:409	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	24	contain	contain	362:368	arg1	fucoidan					353:360	The extracted fucoidan	339:360	The extracted fucoidan	339:360	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	24	contain	contain	362:368	arg2	%					375:375	54.86%	370:375	54.86% of total sugar	370:390	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	24	contain	contain	362:368	arg2	sugar					386:390	total sugar	380:390	total sugar	380:390	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	24	contain	contain	362:368	arg2	sulfate					403:409	sulfate	403:409	sulfate	403:409	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	24	contain	contain	362:368	arg2	%					418:418	3.4%	415:418	3.4% of protein	415:429	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	6	25	theme	isolated	898:905	arg1	fucoidan					907:914	The isolated fucoidan	894:914	The isolated fucoidan	894:914	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	4	26	theme	UV-visible	650:659	arg1	spectroscopy					661:672	UV-visible spectroscopy	650:672	UV-visible spectroscopy	650:672	Further, the structural characterization was done by UV-visible spectroscopy, X-ray diffraction, FT-IR and 1HNMR analysis.					
32446898	1	27	dep	induced	288:294	arg1	formalin					279:286	formalin	279:286	formalin	279:286	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	2	28	theme	total	380:384	arg1	sugar					386:390	total sugar	380:390	total sugar	380:390	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	0	29	from	characterization	22:37	arg1	algae					62:66	brown algae	56:66	brown algae Turbinaria decurrens	56:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	4	30	theme	structural	610:619	arg1	characterization					621:636	the structural characterization	606:636	the structural characterization	606:636	Further, the structural characterization was done by UV-visible spectroscopy, X-ray diffraction, FT-IR and 1HNMR analysis.					
32446898	7	31	from	COX-2	1251:1255	arg1	mice					1287:1290	fucoidan treated mice	1270:1290	fucoidan treated mice	1270:1290	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	8	32	theme	pro-inflammatory	1444:1459	arg1	cytokines					1461:1469	pro-inflammatory cytokines	1444:1469	pro-inflammatory cytokines	1444:1469	The anti-inflammatory effect of fucoidan was attributed to its capacity on modulating the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines.					
32446898	0	33	dep	Turbinaria	68:77	arg1	decurrens					79:87	decurrens	79:87	decurrens	79:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	8	34	theme	anti-inflammatory	1297:1313	arg1	effect					1315:1320	The anti-inflammatory effect	1293:1320	The anti-inflammatory effect of fucoidan	1293:1332	The anti-inflammatory effect of fucoidan was attributed to its capacity on modulating the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines.					
32446898	1	35	theme	present	170:176	arg1	study					178:182	the present study	166:182	the present study	166:182	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	9	36	from	cytotoxicity	1509:1520	arg1	macrophage					1531:1540	IC-21 macrophage	1525:1540	IC-21 macrophage	1525:1540	The fucoidan has reduced LPS induced cytotoxicity in IC-21 macrophage at a dose depended on manner.					
32446898	6	37	theme	SOD	976:978	arg1	activity					1003:1010	the SOD, CAT, GPx, GST and GSH activity	972:1010	the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice	972:1056	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	0	38	theme	in	112:113	arg1	activities					151:160	in vivo and in vitro anti-inflammatory activities	112:160	in vivo and in vitro anti-inflammatory activities	112:160	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	6	39	theme	paw	1015:1017	arg1	tissue					1025:1030	paw edema tissue	1015:1030	paw edema tissue	1015:1030	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	7	40	from	MMP-9	1261:1265	arg1	mice					1287:1290	fucoidan treated mice	1270:1290	fucoidan treated mice	1270:1290	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	7	41	theme	gene	1190:1193	arg1	expression					1195:1204	the gene expression	1186:1204	the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice	1186:1290	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	2	42	theme	sulfate	403:409	arg1	sulfate					403:409	sulfate	403:409	sulfate	403:409	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	42	theme	sulfate	403:409	arg1	sugar					386:390	total sugar	380:390	total sugar	380:390	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	42	theme	sulfate	403:409	arg1	%					418:418	3.4%	415:418	3.4% of protein	415:429	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	42	theme	sulfate	403:409	arg1	%					398:398	23.51%	393:398	23.51% of sulfate	393:409	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	42	theme	sulfate	403:409	arg1	protein					423:429	protein	423:429	protein	423:429	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	42	theme	sulfate	403:409	arg1	%					375:375	54.86%	370:375	54.86% of total sugar	370:390	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	5	43	theme	swelling	831:838	arg1	size					819:822	the size	815:822	the size of paw swelling	815:838	The fucoidan reduced the licking time thereby suggesting anti-nociceptive effect and decreased the size of paw swelling in the formalin induced inflammatory edema condition.					
32446898	6	44	theme	CAT	981:983	arg1	activity					1003:1010	the SOD, CAT, GPx, GST and GSH activity	972:1010	the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice	972:1056	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	6	45	theme	edema	1019:1023	arg1	tissue					1025:1030	paw edema tissue	1015:1030	paw edema tissue	1015:1030	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	1	46	theme	induced	288:294	arg1	paw-edema					296:304	formalin induced paw-edema	279:304	formalin induced paw-edema	279:304	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	7	47	theme	pro-inflammatory	1209:1224	arg1	MMP-9					1261:1265	MMP-9	1261:1265	MMP-9	1261:1265	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	7	47	theme	pro-inflammatory	1209:1224	arg1	COX-2					1251:1255	COX-2	1251:1255	COX-2	1251:1255	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	7	47	theme	pro-inflammatory	1209:1224	arg1	mediators					1226:1234	pro-inflammatory mediators	1209:1234	pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice	1209:1290	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	7	47	theme	pro-inflammatory	1209:1224	arg1	IL-1β					1244:1248	IL-1β	1244:1248	IL-1β	1244:1248	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	7	48	theme	mediators	1226:1234	arg1	expression					1195:1204	the gene expression	1186:1204	the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice	1186:1290	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	7	49	theme	treated	1279:1285	arg1	mice					1287:1290	fucoidan treated mice	1270:1290	fucoidan treated mice	1270:1290	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	0	50	from	algae	62:66	arg1	isolation					11:19	isolation	11:19	isolation	11:19	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	50	from	algae	62:66	arg1	evaluation					93:102	evaluation	93:102	evaluation of it's	93:110	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	50	from	algae	62:66	arg1	fucoidan					42:49	fucoidan	42:49	fucoidan from brown algae Turbinaria decurrens	42:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	50	from	algae	62:66	arg1	characterization					22:37	characterization	22:37	characterization of fucoidan from brown algae Turbinaria decurrens	22:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	7	51	theme	fucoidan	1270:1277	arg1	mice					1287:1290	fucoidan treated mice	1270:1290	fucoidan treated mice	1270:1290	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	1	52	theme	anti-nociception	189:204	arg1	activity					228:235	the anti-nociception and anti-inflammatory activity	185:235	the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model	185:318	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	2	53	theme	protein	423:429	arg1	sulfate					403:409	sulfate	403:409	sulfate	403:409	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	53	theme	protein	423:429	arg1	sugar					386:390	total sugar	380:390	total sugar	380:390	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	53	theme	protein	423:429	arg1	%					418:418	3.4%	415:418	3.4% of protein	415:429	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	53	theme	protein	423:429	arg1	%					398:398	23.51%	393:398	23.51% of sulfate	393:409	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	53	theme	protein	423:429	arg1	protein					423:429	protein	423:429	protein	423:429	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	2	53	theme	protein	423:429	arg1	%					375:375	54.86%	370:375	54.86% of total sugar	370:390	The extracted fucoidan contain 54.86% of total sugar, 23.51% of sulfate and 3.4% of protein.					
32446898	1	54	from	activity	228:235	arg1	paw-edema					296:304	formalin induced paw-edema	279:304	formalin induced paw-edema	279:304	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	1	54	from	activity	228:235	arg1	model					314:318	mice model	309:318	mice model	309:318	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	7	55	theme	transcription	1115:1127	arg1	factor					1129:1134	p65/NF-κB transcription factor	1105:1134	p65/NF-κB transcription factor in the cytosol	1105:1149	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	8	56	theme	NF-κB	1434:1438	arg1	levels					1383:1388	the levels	1379:1388	the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines	1379:1469	The anti-inflammatory effect of fucoidan was attributed to its capacity on modulating the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines.					
32446898	7	57	theme	down	1167:1170	arg1	regulation					1172:1181	down regulation	1167:1181	down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice	1167:1290	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	0	58	theme	fucoidan	42:49	arg1	isolation					11:19	isolation	11:19	isolation	11:19	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	58	theme	fucoidan	42:49	arg1	evaluation					93:102	evaluation	93:102	evaluation of it's	93:110	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	58	theme	fucoidan	42:49	arg1	characterization					22:37	characterization	22:37	characterization of fucoidan from brown algae Turbinaria decurrens	22:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	1	59	theme	mice	309:312	arg1	model					314:318	mice model	309:318	mice model	309:318	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	0	60	theme	in	124:125	arg1	activities					151:160	in vivo and in vitro anti-inflammatory activities	112:160	in vivo and in vitro anti-inflammatory activities	112:160	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	8	61	theme	cytokines	1461:1469	arg1	levels					1383:1388	the levels	1379:1388	the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines	1379:1469	The anti-inflammatory effect of fucoidan was attributed to its capacity on modulating the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines.					
32446898	4	62	theme	1HNMR	704:708	arg1	analysis					710:717	1HNMR analysis	704:717	1HNMR analysis	704:717	Further, the structural characterization was done by UV-visible spectroscopy, X-ray diffraction, FT-IR and 1HNMR analysis.					
32446898	6	63	from	activity	1003:1010	arg1	tissue					1025:1030	paw edema tissue	1015:1030	paw edema tissue	1015:1030	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	6	64	theme	GSH	999:1001	arg1	activity					1003:1010	the SOD, CAT, GPx, GST and GSH activity	972:1010	the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice	972:1056	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	4	65	theme	X-ray	675:679	arg1	diffraction					681:691	X-ray diffraction	675:691	X-ray diffraction	675:691	Further, the structural characterization was done by UV-visible spectroscopy, X-ray diffraction, FT-IR and 1HNMR analysis.					
32446898	1	66	attach	isolated	249:256	arg1	decurrens					266:274	T. decurrens	263:274	T. decurrens	263:274	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	1	66	attach	isolated	249:256	arg2	fucoidan					240:247	fucoidan	240:247	fucoidan isolated from T. decurrens	240:274	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	9	67	theme	induced	1501:1507	arg1	cytotoxicity					1509:1520	reduced LPS induced cytotoxicity	1489:1520	reduced LPS induced cytotoxicity in IC-21 macrophage	1489:1540	The fucoidan has reduced LPS induced cytotoxicity in IC-21 macrophage at a dose depended on manner.					
32446898	3	68	theme	monosaccharide	436:449	arg1	analysis					463:470	The monosaccharide composition analysis	432:470	The monosaccharide composition analysis	432:470	The monosaccharide composition analysis revealed that fucoidan encompassed of fucose (59.3%), galactose (12.6%), mannose (9.6%), rhamnose (6.4%) and xylose (11.4%).					
32446898	6	69	theme	GST	991:993	arg1	activity					1003:1010	the SOD, CAT, GPx, GST and GSH activity	972:1010	the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice	972:1056	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	0	70	theme	anti-inflammatory	133:149	arg1	activities					151:160	in vivo and in vitro anti-inflammatory activities	112:160	in vivo and in vitro anti-inflammatory activities	112:160	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	71	from	evaluation	93:102	arg1	algae					62:66	brown algae	56:66	brown algae Turbinaria decurrens	56:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	0	72	dep	isolation	11:19	arg1	activities					151:160	in vivo and in vitro anti-inflammatory activities	112:160	in vivo and in vitro anti-inflammatory activities	112:160	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	6	73	theme	formalin	1035:1042	arg1	mice					1053:1056	formalin injected mice	1035:1056	formalin injected mice	1035:1056	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	0	74	dep	in	124:125	arg1	vitro					127:131	vitro	127:131	vitro	127:131	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	1	75	theme	anti-inflammatory	210:226	arg1	activity					228:235	the anti-nociception and anti-inflammatory activity	185:235	the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model	185:318	In the present study, the anti-nociception and anti-inflammatory activity of fucoidan isolated from T. decurrens on formalin induced paw-edema in mice model were investigated.					
32446898	7	76	theme	p65/NF-κB	1105:1113	arg1	factor					1129:1134	p65/NF-κB transcription factor	1105:1134	p65/NF-κB transcription factor in the cytosol	1105:1149	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
32446898	6	77	theme	GPx	986:988	arg1	activity					1003:1010	the SOD, CAT, GPx, GST and GSH activity	972:1010	the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice	972:1056	The isolated fucoidan could significantly decreased the MDA and also increase the SOD, CAT, GPx, GST and GSH activity in paw edema tissue of formalin injected mice.					
32446898	8	78	theme	fucoidan	1325:1332	arg1	effect					1315:1320	The anti-inflammatory effect	1293:1320	The anti-inflammatory effect of fucoidan	1293:1332	The anti-inflammatory effect of fucoidan was attributed to its capacity on modulating the levels of enzymatic antioxidants, master regulator NF-κB and pro-inflammatory cytokines.					
32446898	5	79	theme	licking	745:751	arg1	time					753:756	the licking time	741:756	the licking time	741:756	The fucoidan reduced the licking time thereby suggesting anti-nociceptive effect and decreased the size of paw swelling in the formalin induced inflammatory edema condition.					
32446898	0	80	theme	brown	56:60	arg1	algae					62:66	brown algae	56:66	brown algae Turbinaria decurrens	56:87	Studies on isolation, characterization of fucoidan from brown algae Turbinaria decurrens and evaluation of it's in vivo and in vitro anti-inflammatory activities.					
32446898	9	81	contain	has	1485:1487	arg1	fucoidan					1476:1483	The fucoidan	1472:1483	The fucoidan	1472:1483	The fucoidan has reduced LPS induced cytotoxicity in IC-21 macrophage at a dose depended on manner.					
32446898	9	81	contain	has	1485:1487	arg2	cytotoxicity					1509:1520	reduced LPS induced cytotoxicity	1489:1520	reduced LPS induced cytotoxicity in IC-21 macrophage	1489:1540	The fucoidan has reduced LPS induced cytotoxicity in IC-21 macrophage at a dose depended on manner.					
32446898	5	82	theme	anti-nociceptive	777:792	arg1	effect					794:799	anti-nociceptive effect	777:799	anti-nociceptive effect	777:799	The fucoidan reduced the licking time thereby suggesting anti-nociceptive effect and decreased the size of paw swelling in the formalin induced inflammatory edema condition.					
32446898	7	83	theme	fucoidan	1072:1079	arg1	administration					1081:1094	fucoidan administration	1072:1094	fucoidan administration	1072:1094	Furthermore, fucoidan administration retained p65/NF-κB transcription factor in the cytosol thereby showing down regulation of the gene expression of pro-inflammatory mediators such as IL-1β, COX-2 and MMP-9 in fucoidan treated mice.					
34110482	0	0	theme	acidic	90:95	arg1	exopolysaccharide					97:113	acidic exopolysaccharide	90:113	acidic exopolysaccharide	90:113	Production, structural and biochemical characterization relevant to antitumor property of acidic exopolysaccharide produced from Bacillus sp.					
34110482	7	1	with	1.0:1.7:0.8	836:846	arg1	weight					865:870	a molecular weight	853:870	a molecular weight of 3.59 × 105 g/mol	853:890	The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol.					
34110482	3	2	theme	Egyptian	236:243	arg1	seawater					245:252	Egyptian seawater	236:252	Egyptian seawater	236:252	NRC5 grown in Egyptian seawater to use it as natural antitumor therapy.					
34110482	5	3	dep	cyclooxygenase	488:501	arg1	COX-2					514:518	COX-2	514:518	COX-2	514:518	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	3	dep	cyclooxygenase	488:501	arg1	COX-1					504:508	COX-1	504:508	COX-1	504:508	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	4	dep	lines	593:597	arg1	MCF-7					600:604	MCF-7	600:604	MCF-7	600:604	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	4	dep	lines	593:597	arg1	lines					593:597	human breast and prostate cancer cell lines	555:597	human breast and prostate cancer cell lines (MCF-7 and PC3)	555:613	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	4	dep	lines	593:597	arg1	PC3					610:612	PC3	610:612	PC3	610:612	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	10	5	theme	reduced	1292:1298	arg1	cells					1314:1318	reduced viable cancer cells count, tumor volume and weight	1292:1349	reduced viable cancer cells count, tumor volume and weight	1292:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	5	theme	reduced	1292:1298	arg1	weight					1344:1349	weight	1344:1349	weight	1344:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	5	theme	reduced	1292:1298	arg1	count					1320:1324	count	1320:1324	count	1320:1324	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	5	theme	reduced	1292:1298	arg1	volume					1333:1338	tumor volume	1327:1338	tumor volume	1327:1338	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	6	theme	modulated	1352:1360	arg1	components					1368:1377	modulated blood components	1352:1377	modulated blood components	1352:1377	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	8	7	with	comparison	944:953	arg1	celecoxib					979:987	the reference drug celecoxib	960:987	the reference drug celecoxib	960:987	BEPS showed a promising COX-2 inhibitory effect in comparison with the reference drug celecoxib.					
34110482	10	8	theme	blood	1362:1366	arg1	components					1368:1377	modulated blood components	1352:1377	modulated blood components	1352:1377	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	2	9	from	sp	218:219	arg1	production					172:181	the production	168:181	the production of exopolysaccharide from Bacillus sp	168:219	This study targeted the production of exopolysaccharide from Bacillus sp.					
34110482	7	10	attach	presented	724:732	arg2	BEPS					719:722	The BEPS	715:722	The BEPS	715:722	The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol.					
34110482	7	10	attach	presented	724:732	arg1	ratio					827:831	a molar ratio	819:831	a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol	819:890	The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol.					
34110482	6	11	theme	EAC	703:705	arg1	model					708:712	the Ehrlich Ascites Carcinoma (EAC) model	672:712	the Ehrlich Ascites Carcinoma (EAC) model	672:712	In addition, BEPS antitumor activity was tested against the Ehrlich Ascites Carcinoma (EAC) model.					
34110482	0	12	theme	exopolysaccharide	97:113	arg1	property					78:85	antitumor property	68:85	antitumor property of acidic exopolysaccharide	68:113	Production, structural and biochemical characterization relevant to antitumor property of acidic exopolysaccharide produced from Bacillus sp.					
34110482	12	13	contain	containing	1590:1599	arg1	composition					1578:1588	its unique composition	1567:1588	its unique composition containing sulfated moieties and uronic acids	1567:1634	NRC5 showed its antioxidant and anti-inflammatory abilities and antitumor abilities, which may all be attributed to its unique composition containing sulfated moieties and uronic acids.					
34110482	12	13	contain	containing	1590:1599	arg2	acids					1630:1634	uronic acids	1623:1634	uronic acids	1623:1634	NRC5 showed its antioxidant and anti-inflammatory abilities and antitumor abilities, which may all be attributed to its unique composition containing sulfated moieties and uronic acids.					
34110482	12	13	contain	containing	1590:1599	arg2	moieties					1610:1617	sulfated moieties	1601:1617	sulfated moieties	1601:1617	NRC5 showed its antioxidant and anti-inflammatory abilities and antitumor abilities, which may all be attributed to its unique composition containing sulfated moieties and uronic acids.					
34110482	9	14	theme	cells	1085:1089	arg1	cells					1085:1089	MCF-7 and PC3 cells	1071:1089	cells	1085:1089	BEPS appeared efficient anticancer property, where it killed 64.20 and 70.20% of MCF-7 and PC3 cells at 100 µg/ml, respectively (IC50, 76.70, and 70.40 µg/ml, respectively).					
34110482	9	14	theme	cells	1085:1089	arg1	%					1066:1066	64.20 and 70.20%	1051:1066	64.20 and 70.20% of MCF-7 and PC3 cells	1051:1089	BEPS appeared efficient anticancer property, where it killed 64.20 and 70.20% of MCF-7 and PC3 cells at 100 µg/ml, respectively (IC50, 76.70, and 70.40 µg/ml, respectively).					
34110482	10	15	theme	cancer	1307:1312	arg1	cells					1314:1318	reduced viable cancer cells count, tumor volume and weight	1292:1349	reduced viable cancer cells count, tumor volume and weight	1292:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	15	theme	cancer	1307:1312	arg1	weight					1344:1349	weight	1344:1349	weight	1344:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	15	theme	cancer	1307:1312	arg1	count					1320:1324	count	1320:1324	count	1320:1324	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	15	theme	cancer	1307:1312	arg1	volume					1333:1338	tumor volume	1327:1338	tumor volume	1327:1338	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	4	16	theme	biological	298:307	arg1	activities					309:318	The biological activities	294:318	The biological activities of selected exopolysaccharide (BEPS) as an antioxidant, anti-inflammatory and anticancer	294:407	The biological activities of selected exopolysaccharide (BEPS) as an antioxidant, anti-inflammatory and anticancer have been studied.					
34110482	0	17	theme	Bacillus	129:136	arg1	sp					138:139	Bacillus sp	129:139	Bacillus sp	129:139	Production, structural and biochemical characterization relevant to antitumor property of acidic exopolysaccharide produced from Bacillus sp.					
34110482	7	18	theme	molar	821:825	arg1	ratio					827:831	a molar ratio	819:831	a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol	819:890	The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol.					
34110482	10	19	theme	mice	1229:1232	arg1	lifespan					1217:1224	the lifespan	1213:1224	the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation	1213:1416	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	20	theme	viable	1300:1305	arg1	cells					1314:1318	reduced viable cancer cells count, tumor volume and weight	1292:1349	reduced viable cancer cells count, tumor volume and weight	1292:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	20	theme	viable	1300:1305	arg1	weight					1344:1349	weight	1344:1349	weight	1344:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	20	theme	viable	1300:1305	arg1	count					1320:1324	count	1320:1324	count	1320:1324	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	20	theme	viable	1300:1305	arg1	volume					1333:1338	tumor volume	1327:1338	tumor volume	1327:1338	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	6	21	theme	Carcinoma	692:700	arg1	model					708:712	the Ehrlich Ascites Carcinoma (EAC) model	672:712	the Ehrlich Ascites Carcinoma (EAC) model	672:712	In addition, BEPS antitumor activity was tested against the Ehrlich Ascites Carcinoma (EAC) model.					
34110482	10	22	from	20 days	1262:1268	arg1	cells					1314:1318	reduced viable cancer cells count, tumor volume and weight	1292:1349	reduced viable cancer cells count, tumor volume and weight	1292:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	22	from	20 days	1262:1268	arg1	weight					1344:1349	weight	1344:1349	weight	1344:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	22	from	20 days	1262:1268	arg1	count					1320:1324	count	1320:1324	count	1320:1324	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	22	from	20 days	1262:1268	arg1	control					1283:1289	the tumor control	1273:1289	the tumor control	1273:1289	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	22	from	20 days	1262:1268	arg1	cells					1396:1400	white blood cells	1384:1400	white blood cells	1384:1400	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	22	from	20 days	1262:1268	arg1	volume					1333:1338	tumor volume	1327:1338	tumor volume	1327:1338	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	22	from	20 days	1262:1268	arg1	components					1368:1377	modulated blood components	1352:1377	modulated blood components	1352:1377	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	5	23	theme	prostate	572:579	arg1	cancer					581:586	prostate cancer	572:586	prostate cancer	572:586	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	6	24	theme	Ascites	684:690	arg1	model					708:712	the Ehrlich Ascites Carcinoma (EAC) model	672:712	the Ehrlich Ascites Carcinoma (EAC) model	672:712	In addition, BEPS antitumor activity was tested against the Ehrlich Ascites Carcinoma (EAC) model.					
34110482	5	25	from	anticancer	541:550	arg1	MCF-7					600:604	MCF-7	600:604	MCF-7	600:604	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	25	from	anticancer	541:550	arg1	lines					593:597	human breast and prostate cancer cell lines	555:597	human breast and prostate cancer cell lines (MCF-7 and PC3)	555:613	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	25	from	anticancer	541:550	arg1	PC3					610:612	PC3	610:612	PC3	610:612	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	26	theme	cancer	581:586	arg1	MCF-7					600:604	MCF-7	600:604	MCF-7	600:604	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	26	theme	cancer	581:586	arg1	lines					593:597	human breast and prostate cancer cell lines	555:597	human breast and prostate cancer cell lines (MCF-7 and PC3)	555:613	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	26	theme	cancer	581:586	arg1	PC3					610:612	PC3	610:612	PC3	610:612	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	6	27	theme	Ehrlich	676:682	arg1	model					708:712	the Ehrlich Ascites Carcinoma (EAC) model	672:712	the Ehrlich Ascites Carcinoma (EAC) model	672:712	In addition, BEPS antitumor activity was tested against the Ehrlich Ascites Carcinoma (EAC) model.					
34110482	8	28	theme	COX-2	917:921	arg1	effect					934:939	a promising COX-2 inhibitory effect	905:939	a promising COX-2 inhibitory effect	905:939	BEPS showed a promising COX-2 inhibitory effect in comparison with the reference drug celecoxib.					
34110482	10	29	dep	control	1283:1289	arg1	differentiation					1402:1416	differentiation	1402:1416	differentiation	1402:1416	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	5	30	theme	cell	588:591	arg1	MCF-7					600:604	MCF-7	600:604	MCF-7	600:604	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	30	theme	cell	588:591	arg1	lines					593:597	human breast and prostate cancer cell lines	555:597	human breast and prostate cancer cell lines (MCF-7 and PC3)	555:613	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	30	theme	cell	588:591	arg1	PC3					610:612	PC3	610:612	PC3	610:612	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	7	31	theme	molecular	855:863	arg1	weight					865:870	a molecular weight	853:870	a molecular weight of 3.59 × 105 g/mol	853:890	The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol.					
34110482	7	32	theme	mannuronic	800:809	arg1	acid					811:814	mannuronic acid	800:814	mannuronic acid	800:814	The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol.					
34110482	10	33	theme	tumor	1277:1281	arg1	control					1283:1289	the tumor control	1273:1289	the tumor control	1273:1289	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	7	34	theme	1.0:1.7:0.8	836:846	arg1	ratio					827:831	a molar ratio	819:831	a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol	819:890	The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol.					
34110482	10	35	theme	tumor	1327:1331	arg1	cells					1314:1318	reduced viable cancer cells count, tumor volume and weight	1292:1349	reduced viable cancer cells count, tumor volume and weight	1292:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	35	theme	tumor	1327:1331	arg1	volume					1333:1338	tumor volume	1327:1338	tumor volume	1327:1338	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	36	theme	white	1384:1388	arg1	cells					1396:1400	white blood cells	1384:1400	white blood cells	1384:1400	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	8	37	theme	promising	907:915	arg1	effect					934:939	a promising COX-2 inhibitory effect	905:939	a promising COX-2 inhibitory effect	905:939	BEPS showed a promising COX-2 inhibitory effect in comparison with the reference drug celecoxib.					
34110482	11	38	theme	Bacillus	1438:1445	arg1	sp					1447:1448	Bacillus sp	1438:1448	Bacillus sp	1438:1448	BEPS produced from Bacillus sp.					
34110482	10	39	dep	cells	1314:1318	arg1	cells					1314:1318	reduced viable cancer cells count, tumor volume and weight	1292:1349	reduced viable cancer cells count, tumor volume and weight	1292:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	39	dep	cells	1314:1318	arg1	weight					1344:1349	weight	1344:1349	weight	1344:1349	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	39	dep	cells	1314:1318	arg1	count					1320:1324	count	1320:1324	count	1320:1324	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	10	39	dep	cells	1314:1318	arg1	volume					1333:1338	tumor volume	1327:1338	tumor volume	1327:1338	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	9	40	dep	respectively	1105:1116	arg1	76.70					1125:1129	76.70	1125:1129	76.70	1125:1129	BEPS appeared efficient anticancer property, where it killed 64.20 and 70.20% of MCF-7 and PC3 cells at 100 µg/ml, respectively (IC50, 76.70, and 70.40 µg/ml, respectively).					
34110482	9	40	dep	respectively	1105:1116	arg1	70.40 µg/ml					1136:1146	70.40 µg/ml	1136:1146	70.40 µg/ml	1136:1146	BEPS appeared efficient anticancer property, where it killed 64.20 and 70.20% of MCF-7 and PC3 cells at 100 µg/ml, respectively (IC50, 76.70, and 70.40 µg/ml, respectively).					
34110482	9	40	dep	respectively	1105:1116	arg1	IC50					1119:1122	IC50	1119:1122	IC50	1119:1122	BEPS appeared efficient anticancer property, where it killed 64.20 and 70.20% of MCF-7 and PC3 cells at 100 µg/ml, respectively (IC50, 76.70, and 70.40 µg/ml, respectively).					
34110482	9	41	theme	MCF-7	1071:1075	arg1	cells					1085:1089	MCF-7 and PC3 cells	1071:1089	cells	1085:1089	BEPS appeared efficient anticancer property, where it killed 64.20 and 70.20% of MCF-7 and PC3 cells at 100 µg/ml, respectively (IC50, 76.70, and 70.40 µg/ml, respectively).					
34110482	8	42	theme	reference	964:972	arg1	celecoxib					979:987	the reference drug celecoxib	960:987	the reference drug celecoxib	960:987	BEPS showed a promising COX-2 inhibitory effect in comparison with the reference drug celecoxib.					
34110482	12	43	theme	unique	1571:1576	arg1	composition					1578:1588	its unique composition	1567:1588	its unique composition containing sulfated moieties and uronic acids	1567:1634	NRC5 showed its antioxidant and anti-inflammatory abilities and antitumor abilities, which may all be attributed to its unique composition containing sulfated moieties and uronic acids.					
34110482	3	44	theme	natural	267:273	arg1	therapy					285:291	natural antitumor therapy	267:291	natural antitumor therapy	267:291	NRC5 grown in Egyptian seawater to use it as natural antitumor therapy.					
34110482	2	45	theme	exopolysaccharide	186:202	arg1	production					172:181	the production	168:181	the production of exopolysaccharide from Bacillus sp	168:219	This study targeted the production of exopolysaccharide from Bacillus sp.					
34110482	9	46	theme	anticancer	1014:1023	arg1	property					1025:1032	efficient anticancer property	1004:1032	efficient anticancer property	1004:1032	BEPS appeared efficient anticancer property, where it killed 64.20 and 70.20% of MCF-7 and PC3 cells at 100 µg/ml, respectively (IC50, 76.70, and 70.40 µg/ml, respectively).					
34110482	0	47	theme	biochemical	27:37	arg1	characterization					39:54	biochemical characterization	27:54	biochemical characterization	27:54	Production, structural and biochemical characterization relevant to antitumor property of acidic exopolysaccharide produced from Bacillus sp.					
34110482	3	48	theme	antitumor	275:283	arg1	therapy					285:291	natural antitumor therapy	267:291	natural antitumor therapy	267:291	NRC5 grown in Egyptian seawater to use it as natural antitumor therapy.					
34110482	9	49	theme	PC3	1081:1083	arg1	cells					1085:1089	MCF-7 and PC3 cells	1071:1089	cells	1085:1089	BEPS appeared efficient anticancer property, where it killed 64.20 and 70.20% of MCF-7 and PC3 cells at 100 µg/ml, respectively (IC50, 76.70, and 70.40 µg/ml, respectively).					
34110482	8	50	theme	inhibitory	923:932	arg1	effect					934:939	a promising COX-2 inhibitory effect	905:939	a promising COX-2 inhibitory effect	905:939	BEPS showed a promising COX-2 inhibitory effect in comparison with the reference drug celecoxib.					
34110482	5	51	theme	human	555:559	arg1	MCF-7					600:604	MCF-7	600:604	MCF-7	600:604	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	51	theme	human	555:559	arg1	lines					593:597	human breast and prostate cancer cell lines	555:597	human breast and prostate cancer cell lines (MCF-7 and PC3)	555:613	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	51	theme	human	555:559	arg1	PC3					610:612	PC3	610:612	PC3	610:612	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	10	52	theme	blood	1390:1394	arg1	cells					1396:1400	white blood cells	1384:1400	white blood cells	1384:1400	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	7	53	theme	antioxidant	741:751	arg1	activities					753:762	potent antioxidant activities	734:762	potent antioxidant activities	734:762	The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol.					
34110482	5	54	theme	breast	561:566	arg1	MCF-7					600:604	MCF-7	600:604	MCF-7	600:604	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	54	theme	breast	561:566	arg1	lines					593:597	human breast and prostate cancer cell lines	555:597	human breast and prostate cancer cell lines (MCF-7 and PC3)	555:613	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	5	54	theme	breast	561:566	arg1	PC3					610:612	PC3	610:612	PC3	610:612	BEPS was evaluated as an anti-inflammatory in vitro against cyclooxygenase (COX-1 and COX-2) and evaluated as an anticancer on human breast and prostate cancer cell lines (MCF-7 and PC3).					
34110482	9	55	theme	efficient	1004:1012	arg1	property					1025:1032	efficient anticancer property	1004:1032	efficient anticancer property	1004:1032	BEPS appeared efficient anticancer property, where it killed 64.20 and 70.20% of MCF-7 and PC3 cells at 100 µg/ml, respectively (IC50, 76.70, and 70.40 µg/ml, respectively).					
34110482	10	56	theme	antitumor	1179:1187	arg1	ability					1189:1195	antitumor ability	1179:1195	antitumor ability	1179:1195	BEPS exhibited antitumor ability as it prolonged the lifespan of mice to reach 75 days instead of 20 days in the tumor control, reduced viable cancer cells count, tumor volume and weight, modulated blood components, and white blood cells differentiation.					
34110482	12	57	theme	antitumor	1515:1523	arg1	abilities					1525:1533	antitumor abilities	1515:1533	antitumor abilities	1515:1533	NRC5 showed its antioxidant and anti-inflammatory abilities and antitumor abilities, which may all be attributed to its unique composition containing sulfated moieties and uronic acids.					
34110482	8	58	theme	drug	974:977	arg1	celecoxib					979:987	the reference drug celecoxib	960:987	the reference drug celecoxib	960:987	BEPS showed a promising COX-2 inhibitory effect in comparison with the reference drug celecoxib.					
34110482	6	59	theme	antitumor	634:642	arg1	activity					644:651	BEPS antitumor activity	629:651	BEPS antitumor activity	629:651	In addition, BEPS antitumor activity was tested against the Ehrlich Ascites Carcinoma (EAC) model.					
34110482	12	60	theme	antioxidant	1467:1477	arg1	abilities					1501:1509	its antioxidant and anti-inflammatory abilities	1463:1509	its antioxidant and anti-inflammatory abilities	1463:1509	NRC5 showed its antioxidant and anti-inflammatory abilities and antitumor abilities, which may all be attributed to its unique composition containing sulfated moieties and uronic acids.					
34110482	12	61	theme	sulfated	1601:1608	arg1	moieties					1610:1617	sulfated moieties	1601:1617	sulfated moieties	1601:1617	NRC5 showed its antioxidant and anti-inflammatory abilities and antitumor abilities, which may all be attributed to its unique composition containing sulfated moieties and uronic acids.					
34110482	0	62	dep	structural	12:21	arg1	Production					0:9	Production	0:9	Production	0:9	Production, structural and biochemical characterization relevant to antitumor property of acidic exopolysaccharide produced from Bacillus sp.					
34110482	4	63	theme	exopolysaccharide	332:348	arg1	BEPS					351:354	selected exopolysaccharide (BEPS)	323:355	selected exopolysaccharide (BEPS)	323:355	The biological activities of selected exopolysaccharide (BEPS) as an antioxidant, anti-inflammatory and anticancer have been studied.					
34110482	6	64	theme	BEPS	629:632	arg1	activity					644:651	BEPS antitumor activity	629:651	BEPS antitumor activity	629:651	In addition, BEPS antitumor activity was tested against the Ehrlich Ascites Carcinoma (EAC) model.					
34110482	7	65	theme	potent	734:739	arg1	activities					753:762	potent antioxidant activities	734:762	potent antioxidant activities	734:762	The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol.					
34110482	2	66	theme	Bacillus	209:216	arg1	sp					218:219	Bacillus sp	209:219	Bacillus sp	209:219	This study targeted the production of exopolysaccharide from Bacillus sp.					
34110482	4	67	theme	selected	323:330	arg1	BEPS					351:354	selected exopolysaccharide (BEPS)	323:355	selected exopolysaccharide (BEPS)	323:355	The biological activities of selected exopolysaccharide (BEPS) as an antioxidant, anti-inflammatory and anticancer have been studied.					
34110482	7	68	theme	3.59 × 105 g/mol	875:890	arg1	weight					865:870	a molecular weight	853:870	a molecular weight of 3.59 × 105 g/mol	853:890	The BEPS presented potent antioxidant activities, consisted of glucose, mannose, and mannuronic acid in a molar ratio of 1.0:1.7:0.8 with a molecular weight of 3.59 × 105 g/mol.					
34110482	12	69	theme	anti-inflammatory	1483:1499	arg1	abilities					1501:1509	its antioxidant and anti-inflammatory abilities	1463:1509	its antioxidant and anti-inflammatory abilities	1463:1509	NRC5 showed its antioxidant and anti-inflammatory abilities and antitumor abilities, which may all be attributed to its unique composition containing sulfated moieties and uronic acids.					
34110482	0	70	theme	antitumor	68:76	arg1	property					78:85	antitumor property	68:85	antitumor property of acidic exopolysaccharide	68:113	Production, structural and biochemical characterization relevant to antitumor property of acidic exopolysaccharide produced from Bacillus sp.					
34110482	4	71	theme	BEPS	351:354	arg1	activities					309:318	The biological activities	294:318	The biological activities of selected exopolysaccharide (BEPS) as an antioxidant, anti-inflammatory and anticancer	294:407	The biological activities of selected exopolysaccharide (BEPS) as an antioxidant, anti-inflammatory and anticancer have been studied.					
34110482	12	72	theme	uronic	1623:1628	arg1	acids					1630:1634	uronic acids	1623:1634	uronic acids	1623:1634	NRC5 showed its antioxidant and anti-inflammatory abilities and antitumor abilities, which may all be attributed to its unique composition containing sulfated moieties and uronic acids.					
34648094	7	0	theme	matrix	1750:1755	arg1	effect					1757:1762	the matrix effect	1746:1762	the matrix effect	1746:1762	Relative recoveries for spiked samples were obtained in the range 88.4-112.0%, indicating that the matrix effect was insignificant, and good precisions (intra- and inter-day) were also achieved (RSDs < 9.0%, n = 3).					
34648094	7	0	theme	matrix	1750:1755	arg1	insignificant					1768:1780	insignificant	1768:1780	insignificant	1768:1780	Relative recoveries for spiked samples were obtained in the range 88.4-112.0%, indicating that the matrix effect was insignificant, and good precisions (intra- and inter-day) were also achieved (RSDs < 9.0%, n = 3).					
34648094	4	1	theme	number	1084:1089	arg1	cavity					1057:1062	The hydrophobic cavity	1041:1062	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure	1041:1128	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	5	2	theme	extraction	1413:1422	arg1	time					1424:1427	extraction time	1413:1427	extraction time	1413:1427	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	2	theme	extraction	1413:1422	arg1	20 min					1430:1435	20 min	1430:1435	20 min	1430:1435	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	4	3	theme	hydroxyl	1094:1101	arg1	groups					1103:1108	hydroxyl groups	1094:1108	hydroxyl groups	1094:1108	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	5	4	theme	sorbent	1249:1255	arg1	amount					1257:1262	sorbent amount	1249:1262	sorbent amount	1249:1262	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	5	theme	optimized	1228:1236	arg1	condition					1238:1246	the optimized condition	1224:1246	the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0)	1224:1468	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	4	6	theme	pesticides	1176:1185	arg1	co-extraction					1149:1161	the co-extraction	1145:1161	the co-extraction of mentioned pesticides with a wide range of polarity	1145:1215	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	3	7	theme	electron	824:831	arg1	FESEM					845:849	FESEM	845:849	FESEM	845:849	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	7	theme	electron	824:831	arg1	microscopy					833:842	field emission scanning electron microscopy	800:842	field emission scanning electron microscopy (FESEM)	800:850	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	8	theme	mGO/β-CD/MD	632:642	arg1	synthesis					619:627	The successful synthesis	604:627	The successful synthesis of mGO/β-CD/MD	604:642	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	1	9	theme	magnetic	262:269	arg1	oxide					280:284	β-cyclodextrin-functionalized magnetic graphene oxide	232:284	β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD)	232:298	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	9	theme	magnetic	262:269	arg1	composite					332:340	a novel hydrophilic-lipophilic composite	301:340	a novel hydrophilic-lipophilic composite	301:340	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	9	theme	magnetic	262:269	arg1	mGO/β-CD/MD					287:297	mGO/β-CD/MD	287:297	mGO/β-CD/MD	287:297	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	3	10	theme	emission	806:813	arg1	FESEM					845:849	FESEM	845:849	FESEM	845:849	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	10	theme	emission	806:813	arg1	microscopy					833:842	field emission scanning electron microscopy	800:842	field emission scanning electron microscopy (FESEM)	800:850	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	0	11	theme	maltodextrin-	150:162	arg1	oxide					207:211	maltodextrin- and β-cyclodextrin-functionalized graphene oxide	150:211	maltodextrin- and β-cyclodextrin-functionalized graphene oxide	150:211	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	4	12	theme	large	1078:1082	arg1	number					1084:1089	a large number	1076:1089	a large number of hydroxyl groups	1076:1108	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	6	13	theme	Extraction	1546:1555	arg1	efficiencies					1557:1568	Extraction efficiencies	1546:1568	Extraction efficiencies	1546:1568	Extraction efficiencies were in the range 66.4-95.3%, and the limits of detection were 0.01-0.08 μg L-1.					
34648094	6	14	theme	detection	1618:1626	arg1	limits					1608:1613	the limits	1604:1613	the limits of detection	1604:1626	Extraction efficiencies were in the range 66.4-95.3%, and the limits of detection were 0.01-0.08 μg L-1.					
34648094	6	14	theme	detection	1618:1626	arg1	L-1					1646:1648	0.01-0.08 μg L-1	1633:1648	0.01-0.08 μg L-1	1633:1648	Extraction efficiencies were in the range 66.4-95.3%, and the limits of detection were 0.01-0.08 μg L-1.					
34648094	4	15	theme	polarity	1208:1215	arg1	range					1199:1203	a wide range	1192:1203	a wide range of polarity	1192:1215	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	0	16	theme	β-cyclodextrin-functionalized	168:196	arg1	oxide					207:211	maltodextrin- and β-cyclodextrin-functionalized graphene oxide	150:211	maltodextrin- and β-cyclodextrin-functionalized graphene oxide	150:211	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	5	17	theme	desorption	1297:1306	arg1	volume					1316:1321	desorption solvent volume	1297:1321	desorption solvent volume	1297:1321	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	3	18	theme	attenuated	695:704	arg1	reflection-Fourier					712:729	attenuated total reflection-Fourier	695:729	attenuated total reflection-Fourier	695:729	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	7	19	theme	spiked	1675:1680	arg1	samples					1682:1688	spiked samples	1675:1688	spiked samples	1675:1688	Relative recoveries for spiked samples were obtained in the range 88.4-112.0%, indicating that the matrix effect was insignificant, and good precisions (intra- and inter-day) were also achieved (RSDs < 9.0%, n = 3).					
34648094	5	20	dep	condition	1238:1246	arg1	amount					1257:1262	sorbent amount	1249:1262	sorbent amount	1249:1262	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	3	21	theme	vibrating	853:861	arg1	magnetometry					870:881	vibrating sample magnetometry	853:881	vibrating sample magnetometry (VSM)	853:887	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	21	theme	vibrating	853:861	arg1	VSM					884:886	VSM	884:886	VSM	884:886	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	0	22	theme	pesticides	68:77	arg1	extraction					32:41	Magnetic dispersive solid-phase extraction	0:41	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.	0:212	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	1	23	theme	triazines	405:413	arg1	co-extraction					388:400	the co-extraction	384:400	the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis	384:474	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	24	from	samples	444:450	arg1	triazoles					419:427	triazoles	419:427	triazoles	419:427	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	24	from	samples	444:450	arg1	triazines					405:413	triazines	405:413	triazines	405:413	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	24	from	samples	444:450	arg1	co-extraction					388:400	the co-extraction	384:400	the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis	384:474	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	3	25	theme	X-ray	942:946	arg1	EDX					962:964	EDX	962:964	EDX	962:964	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	25	theme	X-ray	942:946	arg1	spectroscopy					948:959	energy-dispersive X-ray spectroscopy	924:959	energy-dispersive X-ray spectroscopy (EDX)	924:965	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	5	26	theme	sample	1448:1453	arg1	solution					1455:1462	sample solution	1448:1462	sample solution	1448:1462	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	0	27	theme	vegetable	84:92	arg1	samples					94:100	vegetable samples	84:100	vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide	84:211	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	1	28	theme	triazoles	419:427	arg1	co-extraction					388:400	the co-extraction	384:400	the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis	384:474	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	29	theme	vegetable	434:442	arg1	samples					444:450	vegetable samples	434:450	vegetable samples before HPLC-UV analysis	434:474	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	8	30	from	pesticides	1971:1980	arg1	tomato					1993:1998	tomato	1993:1998	tomato	1993:1998	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	30	from	pesticides	1971:1980	arg1	potato					1985:1990	potato	1985:1990	potato	1985:1990	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	30	from	pesticides	1971:1980	arg1	samples					2010:2016	corn samples	2005:2016	corn samples	2005:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	0	31	theme	hydrophilic-lipophilic	110:131	arg1	sorbent					133:139	a hydrophilic-lipophilic sorbent	108:139	a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide	108:211	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	7	32	dep	achieved	1836:1843	arg1	n = 3					1859:1863	n = 3	1859:1863	n = 3	1859:1863	Relative recoveries for spiked samples were obtained in the range 88.4-112.0%, indicating that the matrix effect was insignificant, and good precisions (intra- and inter-day) were also achieved (RSDs < 9.0%, n = 3).					
34648094	7	32	dep	achieved	1836:1843	arg1	%					1856:1856	RSDs < 9.0%	1846:1856	RSDs < 9.0%	1846:1856	Relative recoveries for spiked samples were obtained in the range 88.4-112.0%, indicating that the matrix effect was insignificant, and good precisions (intra- and inter-day) were also achieved (RSDs < 9.0%, n = 3).					
34648094	2	33	theme	MD	537:538	arg1	bonding					517:523	chemical bonding	508:523	chemical bonding of β-CD and MD to the surface of mGO	508:560	mGO/β-CD/MD was synthesized by chemical bonding of β-CD and MD to the surface of mGO, using epichlorohydrin (ECH) as a linker.					
34648094	8	34	from	amounts	1960:1966	arg1	tomato					1993:1998	tomato	1993:1998	tomato	1993:1998	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	34	from	amounts	1960:1966	arg1	potato					1985:1990	potato	1985:1990	potato	1985:1990	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	34	from	amounts	1960:1966	arg1	samples					2010:2016	corn samples	2005:2016	corn samples	2005:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	0	35	theme	Magnetic	0:7	arg1	extraction					32:41	Magnetic dispersive solid-phase extraction	0:41	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.	0:212	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	2	36	theme	β-CD	528:531	arg1	bonding					517:523	chemical bonding	508:523	chemical bonding of β-CD and MD to the surface of mGO	508:560	mGO/β-CD/MD was synthesized by chemical bonding of β-CD and MD to the surface of mGO, using epichlorohydrin (ECH) as a linker.					
34648094	0	37	theme	solid-phase	20:30	arg1	extraction					32:41	Magnetic dispersive solid-phase extraction	0:41	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.	0:212	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	4	38	with	pesticides	1176:1185	arg1	range					1199:1203	a wide range	1192:1203	a wide range of polarity	1192:1215	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	4	39	theme	hydrophobic	1045:1055	arg1	cavity					1057:1062	The hydrophobic cavity	1041:1062	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure	1041:1128	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	5	40	theme	range	1497:1501	arg1	r2 ≥ 0.992					1520:1529	r2 ≥ 0.992	1520:1529	r2 ≥ 0.992	1520:1529	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	40	theme	range	1497:1501	arg1	L-1					1515:1517	the range 1.0-1000 μg L-1	1493:1517	the range 1.0-1000 μg L-1 (r2 ≥ 0.992)	1493:1530	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	3	41	theme	infrared	741:748	arg1	spectroscopy					750:761	infrared spectroscopy	741:761	infrared spectroscopy (ATR-FTIR)	741:772	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	42	theme	Barrett-Joyner-Halenda	1002:1023	arg1	analyses					1031:1038	Barrett-Joyner-Halenda (BJH) analyses	1002:1038	Barrett-Joyner-Halenda (BJH) analyses	1002:1038	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	5	43	theme	%	1392:1392	arg1	acid					1407:1410	5% (v/v) acetic acid	1391:1410	5% (v/v) acetic acid	1391:1410	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	8	44	theme	pesticides	1971:1980	arg1	pesticides					1971:1980	pesticides	1971:1980	pesticides in potato, tomato, and corn samples	1971:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	44	theme	pesticides	1971:1980	arg1	amounts					1960:1966	the determination of trace amounts	1933:1966	the determination of trace amounts of pesticides in potato, tomato, and corn samples	1933:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	5	45	theme	good	1471:1474	arg1	linearity					1476:1484	good linearity	1471:1484	good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992)	1471:1530	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	46	contain	containing	1380:1389	arg2	20 min					1430:1435	20 min	1430:1435	20 min	1430:1435	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	46	contain	containing	1380:1389	arg1	methanol/acetonitrile					1352:1372	methanol/acetonitrile	1352:1372	methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution	1352:1462	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	46	contain	containing	1380:1389	arg1	1:1					1375:1377	1:1	1375:1377	1:1	1375:1377	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	46	contain	containing	1380:1389	arg2	acid					1407:1410	5% (v/v) acetic acid	1391:1410	5% (v/v) acetic acid	1391:1410	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	46	contain	containing	1380:1389	arg2	time					1424:1427	extraction time	1413:1427	extraction time	1413:1427	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	46	contain	containing	1380:1389	arg2	pH					1442:1443	pH	1442:1443	pH of sample solution	1442:1462	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	3	47	theme	BJH	1026:1028	arg1	analyses					1031:1038	Barrett-Joyner-Halenda (BJH) analyses	1002:1038	Barrett-Joyner-Halenda (BJH) analyses	1002:1038	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	1	48	from	co-extraction	388:400	arg1	samples					444:450	vegetable samples	434:450	vegetable samples before HPLC-UV analysis	434:474	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	49	theme	hydrophilic-lipophilic	309:330	arg1	composite					332:340	a novel hydrophilic-lipophilic composite	301:340	a novel hydrophilic-lipophilic composite	301:340	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	49	theme	hydrophilic-lipophilic	309:330	arg1	oxide					280:284	β-cyclodextrin-functionalized magnetic graphene oxide	232:284	β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD)	232:298	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	4	50	theme	groups	1103:1108	arg1	number					1084:1089	a large number	1076:1089	a large number of hydroxyl groups	1076:1108	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	4	50	theme	groups	1103:1108	arg1	β-CD					1067:1070	β-CD	1067:1070	β-CD	1067:1070	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	3	51	theme	X-ray	775:779	arg1	diffraction					781:791	X-ray diffraction	775:791	X-ray diffraction (XRD)	775:797	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	51	theme	X-ray	775:779	arg1	XRD					794:796	XRD	794:796	XRD	794:796	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	1	52	used	used	375:378	arg2	Maltodextrin-					214:226	Maltodextrin-	214:226	Maltodextrin-	214:226	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	8	53	from	tomato	1993:1998	arg1	pesticides					1971:1980	pesticides	1971:1980	pesticides in potato, tomato, and corn samples	1971:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	53	from	tomato	1993:1998	arg1	amounts					1960:1966	the determination of trace amounts	1933:1966	the determination of trace amounts of pesticides in potato, tomato, and corn samples	1933:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	4	54	theme	mentioned	1166:1174	arg1	pesticides					1176:1185	mentioned pesticides	1166:1185	mentioned pesticides with a wide range of polarity	1166:1215	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	3	55	theme	field	800:804	arg1	FESEM					845:849	FESEM	845:849	FESEM	845:849	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	55	theme	field	800:804	arg1	microscopy					833:842	field emission scanning electron microscopy	800:842	field emission scanning electron microscopy (FESEM)	800:850	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	7	56	theme	range	1711:1715	arg1	%					1727:1727	the range 88.4-112.0%	1707:1727	the range 88.4-112.0%	1707:1727	Relative recoveries for spiked samples were obtained in the range 88.4-112.0%, indicating that the matrix effect was insignificant, and good precisions (intra- and inter-day) were also achieved (RSDs < 9.0%, n = 3).					
34648094	5	57	theme	acetic	1400:1405	arg1	acid					1407:1410	5% (v/v) acetic acid	1391:1410	5% (v/v) acetic acid	1391:1410	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	1	58	theme	β-cyclodextrin-functionalized	232:260	arg1	oxide					280:284	β-cyclodextrin-functionalized magnetic graphene oxide	232:284	β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD)	232:298	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	58	theme	β-cyclodextrin-functionalized	232:260	arg1	composite					332:340	a novel hydrophilic-lipophilic composite	301:340	a novel hydrophilic-lipophilic composite	301:340	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	58	theme	β-cyclodextrin-functionalized	232:260	arg1	mGO/β-CD/MD					287:297	mGO/β-CD/MD	287:297	mGO/β-CD/MD	287:297	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	3	59	theme	successful	608:617	arg1	synthesis					619:627	The successful synthesis	604:627	The successful synthesis of mGO/β-CD/MD	604:642	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	8	60	theme	the determination	1933:1949	arg1	pesticides					1971:1980	pesticides	1971:1980	pesticides in potato, tomato, and corn samples	1971:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	60	theme	the determination	1933:1949	arg1	amounts					1960:1966	the determination of trace amounts	1933:1966	the determination of trace amounts of pesticides in potato, tomato, and corn samples	1933:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	1	61	theme	graphene	271:278	arg1	oxide					280:284	β-cyclodextrin-functionalized magnetic graphene oxide	232:284	β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD)	232:298	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	61	theme	graphene	271:278	arg1	composite					332:340	a novel hydrophilic-lipophilic composite	301:340	a novel hydrophilic-lipophilic composite	301:340	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	61	theme	graphene	271:278	arg1	mGO/β-CD/MD					287:297	mGO/β-CD/MD	287:297	mGO/β-CD/MD	287:297	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	0	62	from	samples	94:100	arg1	extraction					32:41	Magnetic dispersive solid-phase extraction	0:41	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.	0:212	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	3	63	theme	scanning	815:822	arg1	FESEM					845:849	FESEM	845:849	FESEM	845:849	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	63	theme	scanning	815:822	arg1	microscopy					833:842	field emission scanning electron microscopy	800:842	field emission scanning electron microscopy (FESEM)	800:850	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	64	dep	including	685:693	arg1	transform					731:739	transform	731:739	transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses	731:1038	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	5	65	dep	amount	1257:1262	arg1	solvent					1343:1349	desorption solvent	1332:1349	desorption solvent	1332:1349	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	65	dep	amount	1257:1262	arg1	time					1283:1286	desorption time	1272:1286	desorption time	1272:1286	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	65	dep	amount	1257:1262	arg1	methanol/acetonitrile					1352:1372	methanol/acetonitrile	1352:1372	methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution	1352:1462	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	65	dep	amount	1257:1262	arg1	10 min					1289:1294	10 min	1289:1294	10 min	1289:1294	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	65	dep	amount	1257:1262	arg1	μL					1328:1329	300 μL	1324:1329	300 μL	1324:1329	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	65	dep	amount	1257:1262	arg1	30 mg					1265:1269	30 mg	1265:1269	30 mg	1265:1269	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	65	dep	amount	1257:1262	arg1	1:1					1375:1377	1:1	1375:1377	1:1	1375:1377	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	65	dep	amount	1257:1262	arg1	volume					1316:1321	desorption solvent volume	1297:1321	desorption solvent volume	1297:1321	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	3	66	dep	spectroscopy	750:761	arg1	ATR-FTIR					764:771	ATR-FTIR	764:771	ATR-FTIR	764:771	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	0	67	theme	graphene	198:205	arg1	oxide					207:211	maltodextrin- and β-cyclodextrin-functionalized graphene oxide	150:211	maltodextrin- and β-cyclodextrin-functionalized graphene oxide	150:211	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	8	68	from	potato	1985:1990	arg1	pesticides					1971:1980	pesticides	1971:1980	pesticides in potato, tomato, and corn samples	1971:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	68	from	potato	1985:1990	arg1	amounts					1960:1966	the determination of trace amounts	1933:1966	the determination of trace amounts of pesticides in potato, tomato, and corn samples	1933:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	4	69	theme	wide	1194:1197	arg1	range					1199:1203	a wide range	1192:1203	a wide range of polarity	1192:1215	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	5	70	theme	solvent	1308:1314	arg1	volume					1316:1321	desorption solvent volume	1297:1321	desorption solvent volume	1297:1321	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	7	71	theme	Relative	1651:1658	arg1	recoveries					1660:1669	Relative recoveries	1651:1669	Relative recoveries for spiked samples	1651:1688	Relative recoveries for spiked samples were obtained in the range 88.4-112.0%, indicating that the matrix effect was insignificant, and good precisions (intra- and inter-day) were also achieved (RSDs < 9.0%, n = 3).					
34648094	5	72	theme	desorption	1272:1281	arg1	time					1283:1286	desorption time	1272:1286	desorption time	1272:1286	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	3	73	theme	sample	863:868	arg1	magnetometry					870:881	vibrating sample magnetometry	853:881	vibrating sample magnetometry (VSM)	853:887	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	73	theme	sample	863:868	arg1	VSM					884:886	VSM	884:886	VSM	884:886	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	2	74	theme	mGO	558:560	arg1	surface					547:553	the surface	543:553	the surface of mGO	543:560	mGO/β-CD/MD was synthesized by chemical bonding of β-CD and MD to the surface of mGO, using epichlorohydrin (ECH) as a linker.					
34648094	5	75	theme	solution	1455:1462	arg1	acid					1407:1410	5% (v/v) acetic acid	1391:1410	5% (v/v) acetic acid	1391:1410	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	75	theme	solution	1455:1462	arg1	pH					1442:1443	pH	1442:1443	pH of sample solution	1442:1462	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	75	theme	solution	1455:1462	arg1	20 min					1430:1435	20 min	1430:1435	20 min	1430:1435	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	75	theme	solution	1455:1462	arg1	time					1424:1427	extraction time	1413:1427	extraction time	1413:1427	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	3	76	theme	thermogravimetric	890:906	arg1	TGA					918:920	TGA	918:920	TGA	918:920	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	76	theme	thermogravimetric	890:906	arg1	analysis					908:915	thermogravimetric analysis	890:915	thermogravimetric analysis (TGA)	890:921	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	8	77	theme	developed	1898:1906	arg1	efficient					1919:1927	efficient	1919:1927	efficient	1919:1927	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	77	theme	developed	1898:1906	arg1	method					1908:1913	the developed method	1894:1913	the developed method	1894:1913	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	3	78	theme	energy-dispersive	924:940	arg1	EDX					962:964	EDX	962:964	EDX	962:964	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	3	78	theme	energy-dispersive	924:940	arg1	spectroscopy					948:959	energy-dispersive X-ray spectroscopy	924:959	energy-dispersive X-ray spectroscopy (EDX)	924:965	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	5	79	theme	7.0	1465:1467	arg1	1:1					1375:1377	1:1	1375:1377	1:1	1375:1377	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	79	theme	7.0	1465:1467	arg1	methanol/acetonitrile					1352:1372	methanol/acetonitrile	1352:1372	methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution	1352:1462	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	80	theme	desorption	1332:1341	arg1	solvent					1343:1349	desorption solvent	1332:1349	desorption solvent	1332:1349	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	6	81	theme	range	1582:1586	arg1	%					1597:1597	the range 66.4-95.3%	1578:1597	the range 66.4-95.3%	1578:1597	Extraction efficiencies were in the range 66.4-95.3%, and the limits of detection were 0.01-0.08 μg L-1.					
34648094	0	82	theme	dispersive	9:18	arg1	extraction					32:41	Magnetic dispersive solid-phase extraction	0:41	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.	0:212	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	1	83	theme	HPLC-UV	459:465	arg1	analysis					467:474	HPLC-UV analysis	459:474	HPLC-UV analysis	459:474	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	4	84	theme	β-CD	1067:1070	arg1	cavity					1057:1062	The hydrophobic cavity	1041:1062	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure	1041:1128	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	8	85	theme	of trace	1951:1958	arg1	pesticides					1971:1980	pesticides	1971:1980	pesticides in potato, tomato, and corn samples	1971:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	85	theme	of trace	1951:1958	arg1	amounts					1960:1966	the determination of trace amounts	1933:1966	the determination of trace amounts of pesticides in potato, tomato, and corn samples	1933:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	86	theme	corn	2005:2008	arg1	samples					2010:2016	corn samples	2005:2016	corn samples	2005:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	2	87	theme	chemical	508:515	arg1	bonding					517:523	chemical bonding	508:523	chemical bonding of β-CD and MD to the surface of mGO	508:560	mGO/β-CD/MD was synthesized by chemical bonding of β-CD and MD to the surface of mGO, using epichlorohydrin (ECH) as a linker.					
34648094	0	88	theme	triazole	46:53	arg1	pesticides					68:77	triazole and triazine pesticides	46:77	triazole and triazine pesticides	46:77	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	7	89	theme	inter-day	1815:1823	arg1	precisions					1792:1801	good precisions	1787:1801	good precisions (intra- and inter-day)	1787:1824	Relative recoveries for spiked samples were obtained in the range 88.4-112.0%, indicating that the matrix effect was insignificant, and good precisions (intra- and inter-day) were also achieved (RSDs < 9.0%, n = 3).					
34648094	5	90	theme	1.0-1000 μg	1503:1513	arg1	r2 ≥ 0.992					1520:1529	r2 ≥ 0.992	1520:1529	r2 ≥ 0.992	1520:1529	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	5	90	theme	1.0-1000 μg	1503:1513	arg1	L-1					1515:1517	the range 1.0-1000 μg L-1	1493:1517	the range 1.0-1000 μg L-1 (r2 ≥ 0.992)	1493:1530	Under the optimized condition (sorbent amount, 30 mg; desorption time, 10 min; desorption solvent volume, 300 μL; desorption solvent, methanol/acetonitrile (1:1) containing 5% (v/v) acetic acid; extraction time, 20 min; and pH of sample solution, 7.0), good linearity within the range 1.0-1000 μg L-1 (r2 ≥ 0.992) was achieved.					
34648094	3	91	theme	total	706:710	arg1	reflection-Fourier					712:729	attenuated total reflection-Fourier	695:729	attenuated total reflection-Fourier	695:729	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	0	92	theme	triazine	59:66	arg1	pesticides					68:77	triazole and triazine pesticides	46:77	triazole and triazine pesticides	46:77	Magnetic dispersive solid-phase extraction of triazole and triazine pesticides from vegetable samples using a hydrophilic-lipophilic sorbent based on maltodextrin- and β-cyclodextrin-functionalized graphene oxide.					
34648094	4	93	from	cavity	1057:1062	arg1	structure					1120:1128	the MD structure	1113:1128	the MD structure	1113:1128	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	7	94	theme	intra-	1804:1809	arg1	precisions					1792:1801	good precisions	1787:1801	good precisions (intra- and inter-day)	1787:1824	Relative recoveries for spiked samples were obtained in the range 88.4-112.0%, indicating that the matrix effect was insignificant, and good precisions (intra- and inter-day) were also achieved (RSDs < 9.0%, n = 3).					
34648094	7	95	theme	good	1787:1790	arg1	precisions					1792:1801	good precisions	1787:1801	good precisions (intra- and inter-day)	1787:1824	Relative recoveries for spiked samples were obtained in the range 88.4-112.0%, indicating that the matrix effect was insignificant, and good precisions (intra- and inter-day) were also achieved (RSDs < 9.0%, n = 3).					
34648094	3	96	theme	characterization	661:676	arg1	tests					678:682	characterization tests	661:682	characterization tests	661:682	The successful synthesis of mGO/β-CD/MD was confirmed by characterization tests, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), thermogravimetric analysis (TGA), energy-dispersive X-ray spectroscopy (EDX), Brunauer-Emmett-Teller (BET), and Barrett-Joyner-Halenda (BJH) analyses.					
34648094	8	97	from	samples	2010:2016	arg1	pesticides					1971:1980	pesticides	1971:1980	pesticides in potato, tomato, and corn samples	1971:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	8	97	from	samples	2010:2016	arg1	amounts					1960:1966	the determination of trace amounts	1933:1966	the determination of trace amounts of pesticides in potato, tomato, and corn samples	1933:2016	The results confirmed that the developed method was efficient for the determination of trace amounts of pesticides in potato, tomato, and corn samples.					
34648094	4	98	theme	MD	1117:1118	arg1	structure					1120:1128	the MD structure	1113:1128	the MD structure	1113:1128	The hydrophobic cavity of β-CD and a large number of hydroxyl groups on the MD structure contributed to the co-extraction of mentioned pesticides with a wide range of polarity.					
34648094	1	99	theme	novel	303:307	arg1	composite					332:340	a novel hydrophilic-lipophilic composite	301:340	a novel hydrophilic-lipophilic composite	301:340	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
34648094	1	99	theme	novel	303:307	arg1	oxide					280:284	β-cyclodextrin-functionalized magnetic graphene oxide	232:284	β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD)	232:298	Maltodextrin- and β-cyclodextrin-functionalized magnetic graphene oxide (mGO/β-CD/MD), a novel hydrophilic-lipophilic composite, was successfully fabricated and used for the co-extraction of triazines and triazoles from vegetable samples before HPLC-UV analysis.					
32035148	10	0	theme	healing	1604:1610	arg1	applications					1612:1623	wound healing applications	1598:1623	wound healing applications	1598:1623	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	5	1	theme	composites	702:711	arg1	RESULTS					582:588	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability	582:676	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites	582:711	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	1	theme	composites	702:711	arg1	absorption					600:609	The water absorption	590:609	The water absorption	590:609	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	1	theme	composites	702:711	arg1	strength					623:630	mechanical strength	612:630	mechanical strength	612:630	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	1	theme	composites	702:711	arg1	resistance					645:654	degradation resistance	633:654	degradation resistance	633:654	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	1	theme	composites	702:711	arg1	stability					668:676	thermal stability	660:676	thermal stability	660:676	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	7	2	theme	mouse	1041:1045	arg1	fibroblasts					1047:1057	mouse fibroblasts	1041:1057	mouse fibroblasts	1041:1057	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	2	theme	mouse	1041:1045	arg1	material					1087:1094	the composite material	1073:1094	the composite material of rCOL (5% HA) group (P < 0.05)	1073:1127	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	1	3	theme	composite	156:164	arg1	scaffolds					166:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds	97:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds	97:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	9	4	theme	thermal	1450:1456	arg1	stability					1458:1466	thermal stability	1450:1466	thermal stability	1450:1466	Compared with rCOL materials, rCOL-HA has increased mechanical strength, water absorption and thermal stability.					
32035148	10	5	theme	wound	1598:1602	arg1	healing					1604:1610	wound healing	1598:1610	wound healing applications	1598:1623	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	10	6	contain	have	1530:1533	arg1	biocompatibility					1473:1488	biocompatibility	1473:1488	biocompatibility	1473:1488	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	10	6	contain	have	1530:1533	arg1	proliferation					1505:1517	fibroblast proliferation	1494:1517	fibroblast proliferation	1494:1517	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	10	6	contain	have	1530:1533	arg2	performance					1556:1566	excellent biological performance	1535:1566	excellent biological performance	1535:1566	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	10	7	theme	fibroblast	1494:1503	arg1	proliferation					1505:1517	fibroblast proliferation	1494:1517	fibroblast proliferation	1494:1517	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	6	8	theme	pore	883:886	arg1	distribution					888:899	uniform pore distribution	875:899	uniform pore distribution	875:899	Scanning electron microscopy (SEM) revealed that the composites formed a three-dimensional network structure with uniform pore distribution.					
32035148	6	9	theme	network	852:858	arg1	structure					860:868	a three-dimensional network structure	832:868	a three-dimensional network structure with uniform pore distribution	832:899	Scanning electron microscopy (SEM) revealed that the composites formed a three-dimensional network structure with uniform pore distribution.					
32035148	7	10	dep	adhesion	990:997	arg1	effects					1017:1023	effects	1017:1023	effects	1017:1023	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	4	11	dep	cytotoxicity	496:507	arg1	effects					553:559	effects	553:559	effects	553:559	Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects were also evaluated.					
32035148	6	12	theme	uniform	875:881	arg1	distribution					888:899	uniform pore distribution	875:899	uniform pore distribution	875:899	Scanning electron microscopy (SEM) revealed that the composites formed a three-dimensional network structure with uniform pore distribution.					
32035148	10	13	theme	rCOL-HA	1522:1528	arg1	biocompatibility					1473:1488	biocompatibility	1473:1488	biocompatibility	1473:1488	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	10	13	theme	rCOL-HA	1522:1528	arg1	proliferation					1505:1517	fibroblast proliferation	1494:1517	fibroblast proliferation	1494:1517	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	9	14	contain	has	1394:1396	arg2	strength					1419:1426	increased mechanical strength	1398:1426	increased mechanical strength	1398:1426	Compared with rCOL materials, rCOL-HA has increased mechanical strength, water absorption and thermal stability.					
32035148	9	14	contain	has	1394:1396	arg2	stability					1458:1466	thermal stability	1450:1466	thermal stability	1450:1466	Compared with rCOL materials, rCOL-HA has increased mechanical strength, water absorption and thermal stability.					
32035148	9	14	contain	has	1394:1396	arg1	rCOL-HA					1386:1392	rCOL-HA	1386:1392	rCOL-HA	1386:1392	Compared with rCOL materials, rCOL-HA has increased mechanical strength, water absorption and thermal stability.					
32035148	9	14	contain	has	1394:1396	arg2	absorption					1435:1444	water absorption	1429:1444	water absorption	1429:1444	Compared with rCOL materials, rCOL-HA has increased mechanical strength, water absorption and thermal stability.					
32035148	5	15	theme	thermal	660:666	arg1	RESULTS					582:588	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability	582:676	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites	582:711	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	15	theme	thermal	660:666	arg1	stability					668:676	thermal stability	660:676	thermal stability	660:676	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	6	16	theme	electron	770:777	arg1	SEM					791:793	SEM	791:793	SEM	791:793	Scanning electron microscopy (SEM) revealed that the composites formed a three-dimensional network structure with uniform pore distribution.					
32035148	6	16	theme	electron	770:777	arg1	microscopy					779:788	Scanning electron microscopy	761:788	Scanning electron microscopy (SEM)	761:794	Scanning electron microscopy (SEM) revealed that the composites formed a three-dimensional network structure with uniform pore distribution.					
32035148	4	17	dep	in	487:488	arg1	vitro					490:494	vitro	490:494	vitro	490:494	Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects were also evaluated.					
32035148	4	18	theme	cell	510:513	arg1	adhesion					515:522	cell adhesion	510:522	cell adhesion	510:522	Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects were also evaluated.					
32035148	10	19	theme	excellent	1535:1543	arg1	performance					1556:1566	excellent biological performance	1535:1566	excellent biological performance	1535:1566	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	6	20	theme	Scanning	761:768	arg1	SEM					791:793	SEM	791:793	SEM	791:793	Scanning electron microscopy (SEM) revealed that the composites formed a three-dimensional network structure with uniform pore distribution.					
32035148	6	20	theme	Scanning	761:768	arg1	microscopy					779:788	Scanning electron microscopy	761:788	Scanning electron microscopy (SEM)	761:794	Scanning electron microscopy (SEM) revealed that the composites formed a three-dimensional network structure with uniform pore distribution.					
32035148	0	21	theme	collagen-hyaluronic	51:69	arg1	composite					76:84	recombinant collagen-hyaluronic acid composite	39:84	recombinant collagen-hyaluronic acid composite	39:84	Assessment of biological properties of recombinant collagen-hyaluronic acid composite scaffolds.					
32035148	7	22	theme	composite	1077:1085	arg1	fibroblasts					1047:1057	mouse fibroblasts	1041:1057	mouse fibroblasts	1041:1057	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	22	theme	composite	1077:1085	arg1	material					1087:1094	the composite material	1073:1094	the composite material of rCOL (5% HA) group (P < 0.05)	1073:1127	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	23	dep	minimal	941:947	arg1	grade					950:954	grade I	950:956	grade I	950:956	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	23	dep	minimal	941:947	arg1	minimal					941:947	minimal	941:947	minimal	941:947	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	23	dep	minimal	941:947	arg1	cytotoxicity					906:917	The cytotoxicity	902:917	The cytotoxicity of the composites	902:935	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	5	24	theme	mechanical	612:621	arg1	RESULTS					582:588	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability	582:676	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites	582:711	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	24	theme	mechanical	612:621	arg1	strength					623:630	mechanical strength	612:630	mechanical strength	612:630	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	1	25	theme	OBJECTIVE	97:105	arg1	acid					146:149	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid	97:149	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds	97:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	1	25	theme	OBJECTIVE	97:105	arg1	HA					152:153	HA	152:153	HA	152:153	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	8	26	theme	good	1273:1276	arg1	interaction					1278:1288	good interaction	1273:1288	good interaction between the material and cells	1273:1319	CONCLUSION The rCOL-HA composite prepared via lyophilization after cross-linking is characterized by high porosity, high water absorption, and good interaction between the material and cells, as well as good biodegradability.					
32035148	10	27	dep	biocompatibility	1473:1488	arg1	The					1469:1471	The	1469:1471	The	1469:1471	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	8	28	theme	high	1231:1234	arg1	porosity					1236:1243	high porosity	1231:1243	high porosity	1231:1243	CONCLUSION The rCOL-HA composite prepared via lyophilization after cross-linking is characterized by high porosity, high water absorption, and good interaction between the material and cells, as well as good biodegradability.					
32035148	5	29	theme	prepared	685:692	arg1	composites					702:711	the prepared rCOL-HA composites	681:711	the prepared rCOL-HA composites	681:711	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	6	30	theme	three-dimensional	834:850	arg1	structure					860:868	a three-dimensional network structure	832:868	a three-dimensional network structure with uniform pore distribution	832:899	Scanning electron microscopy (SEM) revealed that the composites formed a three-dimensional network structure with uniform pore distribution.					
32035148	1	31	theme	Recombinant	107:117	arg1	acid					146:149	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid	97:149	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds	97:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	1	31	theme	Recombinant	107:117	arg1	HA					152:153	HA	152:153	HA	152:153	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	6	32	with	structure	860:868	arg1	distribution					888:899	uniform pore distribution	875:899	uniform pore distribution	875:899	Scanning electron microscopy (SEM) revealed that the composites formed a three-dimensional network structure with uniform pore distribution.					
32035148	9	33	theme	rCOL	1370:1373	arg1	materials					1375:1383	rCOL materials	1370:1383	rCOL materials	1370:1383	Compared with rCOL materials, rCOL-HA has increased mechanical strength, water absorption and thermal stability.					
32035148	5	34	theme	rCOL-HA	694:700	arg1	composites					702:711	the prepared rCOL-HA composites	681:711	the prepared rCOL-HA composites	681:711	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	1	35	theme	collagen	119:126	arg1	acid					146:149	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid	97:149	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds	97:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	1	35	theme	collagen	119:126	arg1	HA					152:153	HA	152:153	HA	152:153	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	0	36	theme	properties	25:34	arg1	Assessment					0:9	Assessment	0:9	Assessment of biological properties of recombinant collagen-hyaluronic acid composite	0:84	Assessment of biological properties of recombinant collagen-hyaluronic acid composite scaffolds.					
32035148	8	37	dep	CONCLUSION	1130:1139	arg1	characterized					1214:1226	characterized	1214:1226	is characterized by high porosity, high water absorption, and good interaction between the material and cells, as well as good biodegradability	1211:1353	CONCLUSION The rCOL-HA composite prepared via lyophilization after cross-linking is characterized by high porosity, high water absorption, and good interaction between the material and cells, as well as good biodegradability.					
32035148	5	38	theme	degradation	633:643	arg1	RESULTS					582:588	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability	582:676	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites	582:711	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	38	theme	degradation	633:643	arg1	resistance					645:654	degradation resistance	633:654	degradation resistance	633:654	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	39	dep	RESULTS	582:588	arg1	RESULTS					582:588	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability	582:676	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites	582:711	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	39	dep	RESULTS	582:588	arg1	absorption					600:609	The water absorption	590:609	The water absorption	590:609	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	39	dep	RESULTS	582:588	arg1	strength					623:630	mechanical strength	612:630	mechanical strength	612:630	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	39	dep	RESULTS	582:588	arg1	resistance					645:654	degradation resistance	633:654	degradation resistance	633:654	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	39	dep	RESULTS	582:588	arg1	stability					668:676	thermal stability	660:676	thermal stability	660:676	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	2	40	dep	METHODS	245:251	arg1	formulated					294:303	formulated	294:303	were formulated via lyophilization	289:322	METHODS The rCOL and HA composite scaffolds were formulated via lyophilization.					
32035148	3	41	theme	surface	412:418	arg1	modification					420:431	surface modification	412:431	surface modification	412:431	The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates.					
32035148	7	42	theme	group	1112:1116	arg1	fibroblasts					1047:1057	mouse fibroblasts	1041:1057	mouse fibroblasts	1041:1057	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	42	theme	group	1112:1116	arg1	material					1087:1094	the composite material	1073:1094	the composite material of rCOL (5% HA) group (P < 0.05)	1073:1127	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	1	43	theme	rCOL	129:132	arg1	acid					146:149	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid	97:149	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds	97:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	1	43	theme	rCOL	129:132	arg1	HA					152:153	HA	152:153	HA	152:153	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	0	44	theme	recombinant	39:49	arg1	composite					76:84	recombinant collagen-hyaluronic acid composite	39:84	recombinant collagen-hyaluronic acid composite	39:84	Assessment of biological properties of recombinant collagen-hyaluronic acid composite scaffolds.					
32035148	7	45	theme	%	1106:1106	arg1	HA					1108:1109	5% HA	1105:1109	5% HA	1105:1109	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	45	theme	%	1106:1106	arg1	group					1112:1116	rCOL (5% HA) group	1099:1116	rCOL (5% HA) group (P < 0.05)	1099:1127	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	4	46	theme	in	487:488	arg1	cytotoxicity					496:507	in vitro cytotoxicity	487:507	in vitro cytotoxicity	487:507	Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects were also evaluated.					
32035148	2	47	theme	rCOL	257:260	arg1	scaffolds					279:287	The rCOL and HA composite scaffolds	253:287	The rCOL and HA composite scaffolds	253:287	METHODS The rCOL and HA composite scaffolds were formulated via lyophilization.					
32035148	1	48	theme	biological	222:231	arg1	properties					233:242	their biological properties	216:242	their biological properties	216:242	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	8	49	theme	water	1251:1255	arg1	absorption					1257:1266	high water absorption	1246:1266	high water absorption	1246:1266	CONCLUSION The rCOL-HA composite prepared via lyophilization after cross-linking is characterized by high porosity, high water absorption, and good interaction between the material and cells, as well as good biodegradability.					
32035148	10	50	theme	biological	1545:1554	arg1	performance					1556:1566	excellent biological performance	1535:1566	excellent biological performance	1535:1566	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	8	51	theme	high	1246:1249	arg1	absorption					1257:1266	high water absorption	1246:1266	high water absorption	1246:1266	CONCLUSION The rCOL-HA composite prepared via lyophilization after cross-linking is characterized by high porosity, high water absorption, and good interaction between the material and cells, as well as good biodegradability.					
32035148	7	52	theme	composites	926:935	arg1	grade					950:954	grade I	950:956	grade I	950:956	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	52	theme	composites	926:935	arg1	minimal					941:947	minimal	941:947	minimal	941:947	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	52	theme	composites	926:935	arg1	cytotoxicity					906:917	The cytotoxicity	902:917	The cytotoxicity of the composites	902:935	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	0	53	theme	acid	71:74	arg1	composite					76:84	recombinant collagen-hyaluronic acid composite	39:84	recombinant collagen-hyaluronic acid composite	39:84	Assessment of biological properties of recombinant collagen-hyaluronic acid composite scaffolds.					
32035148	9	54	theme	increased	1398:1406	arg1	strength					1419:1426	increased mechanical strength	1398:1426	increased mechanical strength	1398:1426	Compared with rCOL materials, rCOL-HA has increased mechanical strength, water absorption and thermal stability.					
32035148	0	55	theme	composite	76:84	arg1	properties					25:34	biological properties	14:34	biological properties of recombinant collagen-hyaluronic acid composite	14:84	Assessment of biological properties of recombinant collagen-hyaluronic acid composite scaffolds.					
32035148	5	56	theme	water	594:598	arg1	RESULTS					582:588	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability	582:676	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites	582:711	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	5	56	theme	water	594:598	arg1	absorption					600:609	The water absorption	590:609	The water absorption	590:609	RESULTS The water absorption, mechanical strength, degradation resistance and thermal stability of the prepared rCOL-HA composites were improved over that of the control studied.					
32035148	8	57	dep	material	1302:1309	arg1	the					1298:1300	the	1298:1300	the	1298:1300	CONCLUSION The rCOL-HA composite prepared via lyophilization after cross-linking is characterized by high porosity, high water absorption, and good interaction between the material and cells, as well as good biodegradability.					
32035148	3	58	theme	degradation	438:448	arg1	rates					450:454	degradation rates	438:454	degradation rates	438:454	The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates.					
32035148	9	59	theme	mechanical	1408:1417	arg1	strength					1419:1426	increased mechanical strength	1398:1426	increased mechanical strength	1398:1426	Compared with rCOL materials, rCOL-HA has increased mechanical strength, water absorption and thermal stability.					
32035148	7	60	theme	5	1105:1105	arg1	%					1106:1106	%	1106:1106	%	1106:1106	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	1	61	theme	-hyaluronic	134:144	arg1	acid					146:149	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid	97:149	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds	97:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	1	61	theme	-hyaluronic	134:144	arg1	HA					152:153	HA	152:153	HA	152:153	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	7	62	theme	strong	983:988	arg1	adhesion					990:997	strong adhesion	983:997	strong adhesion	983:997	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	10	63	theme	new	1581:1583	arg1	material					1585:1592	a new material	1579:1592	a new material for wound healing applications	1579:1623	The biocompatibility and fibroblast proliferation of rCOL-HA have excellent biological performance, providing a new material for wound healing applications.					
32035148	4	64	theme	Biological	457:466	arg1	migration					543:551	migration	543:551	migration	543:551	Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects were also evaluated.					
32035148	4	64	theme	Biological	457:466	arg1	proliferation					525:537	proliferation	525:537	proliferation	525:537	Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects were also evaluated.					
32035148	4	64	theme	Biological	457:466	arg1	cytotoxicity					496:507	in vitro cytotoxicity	487:507	in vitro cytotoxicity	487:507	Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects were also evaluated.					
32035148	4	64	theme	Biological	457:466	arg1	properties					468:477	Biological properties	457:477	Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects	457:559	Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects were also evaluated.					
32035148	4	64	theme	Biological	457:466	arg1	adhesion					515:522	cell adhesion	510:522	cell adhesion	510:522	Biological properties such as in vitro cytotoxicity, cell adhesion, proliferation and migration effects were also evaluated.					
32035148	8	65	theme	rCOL-HA	1145:1151	arg1	composite					1153:1161	The rCOL-HA composite	1141:1161	The rCOL-HA composite prepared via lyophilization after cross-linking	1141:1209	CONCLUSION The rCOL-HA composite prepared via lyophilization after cross-linking is characterized by high porosity, high water absorption, and good interaction between the material and cells, as well as good biodegradability.					
32035148	1	66	theme	acid	146:149	arg1	scaffolds					166:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds	97:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds	97:174	OBJECTIVE Recombinant collagen (rCOL)-hyaluronic acid (HA) composite scaffolds were prepared to thoroughly investigate their biological properties.					
32035148	7	67	theme	rCOL	1099:1102	arg1	HA					1108:1109	5% HA	1105:1109	5% HA	1105:1109	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	67	theme	rCOL	1099:1102	arg1	P < 0.05					1119:1126	P < 0.05	1119:1126	P < 0.05	1119:1126	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	7	67	theme	rCOL	1099:1102	arg1	group					1112:1116	rCOL (5% HA) group	1099:1116	rCOL (5% HA) group (P < 0.05)	1099:1127	The cytotoxicity of the composites was minimal (grade I) and the material showed strong adhesion and proliferation effects when grown with mouse fibroblasts, particularly the composite material of rCOL (5% HA) group (P < 0.05).					
32035148	9	68	theme	water	1429:1433	arg1	absorption					1435:1444	water absorption	1429:1444	water absorption	1429:1444	Compared with rCOL materials, rCOL-HA has increased mechanical strength, water absorption and thermal stability.					
32035148	2	69	theme	composite	269:277	arg1	scaffolds					279:287	The rCOL and HA composite scaffolds	253:287	The rCOL and HA composite scaffolds	253:287	METHODS The rCOL and HA composite scaffolds were formulated via lyophilization.					
32035148	8	70	theme	good	1333:1336	arg1	biodegradability					1338:1353	good biodegradability	1333:1353	good biodegradability	1333:1353	CONCLUSION The rCOL-HA composite prepared via lyophilization after cross-linking is characterized by high porosity, high water absorption, and good interaction between the material and cells, as well as good biodegradability.					
32035148	3	71	theme	various	362:368	arg1	modification					420:431	surface modification	412:431	surface modification	412:431	The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates.					
32035148	3	71	theme	various	362:368	arg1	rates					450:454	degradation rates	438:454	degradation rates	438:454	The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates.					
32035148	3	71	theme	various	362:368	arg1	properties					380:389	various materials properties	362:389	various materials properties	362:389	The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates.					
32035148	3	71	theme	various	362:368	arg1	porosity					402:409	porosity	402:409	porosity	402:409	The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates.					
32035148	2	72	theme	HA	266:267	arg1	scaffolds					279:287	The rCOL and HA composite scaffolds	253:287	The rCOL and HA composite scaffolds	253:287	METHODS The rCOL and HA composite scaffolds were formulated via lyophilization.					
32035148	3	73	theme	materials	370:378	arg1	modification					420:431	surface modification	412:431	surface modification	412:431	The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates.					
32035148	3	73	theme	materials	370:378	arg1	rates					450:454	degradation rates	438:454	degradation rates	438:454	The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates.					
32035148	3	73	theme	materials	370:378	arg1	properties					380:389	various materials properties	362:389	various materials properties	362:389	The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates.					
32035148	3	73	theme	materials	370:378	arg1	porosity					402:409	porosity	402:409	porosity	402:409	The scaffolds were characterized for various materials properties, including porosity, surface modification, and degradation rates.					
32035148	0	74	theme	biological	14:23	arg1	properties					25:34	biological properties	14:34	biological properties of recombinant collagen-hyaluronic acid composite	14:84	Assessment of biological properties of recombinant collagen-hyaluronic acid composite scaffolds.					
33310387	0	0	theme	sugarcane	92:100	arg1	bagasse					102:108	sugarcane bagasse	92:108	sugarcane bagasse	92:108	Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse.					
33310387	1	1	theme	variables	322:330	arg1	range					313:317	a wide range	306:317	a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL)	306:514	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	2	2	theme	optimized	660:668	arg1	conditions					670:679	optimized conditions	660:679	optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading)	660:748	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	4	3	theme	robust	918:923	arg1	design					925:930	This robust design	913:930	This robust design of experiments	913:945	This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.					
33310387	1	4	theme	Composite	245:253	arg1	design					272:277	a Central Composite Orthogonal (CCO) design	235:277	a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL)	235:514	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	2	5	with	NaOH	693:696	arg1	%					729:729	12.5%	725:729	12.5% of enzyme loading	725:747	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	2	5	with	NaOH	693:696	arg1	loading					741:747	enzyme loading	734:747	enzyme loading	734:747	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	4	6	theme	experiments	935:945	arg1	design					925:930	This robust design	913:930	This robust design of experiments	913:945	This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.					
33310387	1	7	theme	Orthogonal	255:264	arg1	design					272:277	a Central Composite Orthogonal (CCO) design	235:277	a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL)	235:514	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	8	theme	alkaline	336:343	arg1	NaOH					359:362	NaOH	359:362	NaOH	359:362	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	8	theme	alkaline	336:343	arg1	pretreatment					345:356	alkaline pretreatment	336:356	alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min)	336:422	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	2	9	theme	conditions	544:553	arg1	total					519:523	A total	517:523	A total of 46 experimental conditions	517:553	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	1	10	dep	design	272:277	arg1	evaluate					297:304	evaluate	297:304	to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL)	279:514	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	4	11	theme	combined	1055:1062	arg1	approach					1064:1071	this combined approach	1050:1071	this combined approach	1050:1071	This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.					
33310387	2	12	theme	experimental	531:542	arg1	conditions					544:553	46 experimental conditions	528:553	46 experimental conditions	528:553	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	3	13	theme	cellulose	881:889	arg1	enrichment					891:900	cellulose enrichment	881:900	cellulose enrichment (25-110%)	881:910	Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25-110%).					
33310387	3	13	theme	cellulose	881:889	arg1	%					909:909	25-110%	903:909	25-110%	903:909	Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25-110%).					
33310387	2	14	dep	conditions	670:679	arg1	NaOH					693:696	0.25 mol/L NaOH	682:696	0.25 mol/L NaOH at 202 °C for 40 min	682:717	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	1	15	theme	CCO	267:269	arg1	design					272:277	a Central Composite Orthogonal (CCO) design	235:277	a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL)	235:514	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	3	16	theme	Biomass	751:757	arg1	analyses					773:780	Biomass compositional analyses	751:780	Biomass compositional analyses	751:780	Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25-110%).					
33310387	2	17	theme	sugar	586:590	arg1	423 mg/g					599:606	423 mg/g	599:606	423 mg/g	599:606	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	2	17	theme	sugar	586:590	arg1	yield					592:596	the maximum sugar yield	574:596	the maximum sugar yield (423 mg/g)	574:607	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	1	18	from	bagasse	156:162	arg1	release					133:139	the sugar release	123:139	the sugar release from sugarcane bagasse	123:162	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	4	19	theme	bagasse	1015:1021	arg1	efficiency					991:1000	enzymatic hydrolysis efficiency	970:1000	enzymatic hydrolysis efficiency of sugarcane bagasse	970:1021	This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.					
33310387	1	20	theme	high-resolution	167:181	arg1	FFD					212:214	FFD	212:214	FFD	212:214	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	20	theme	high-resolution	167:181	arg1	Design					204:209	a high-resolution Fractional Factorial Design	165:209	a high-resolution Fractional Factorial Design (FFD)	165:215	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	2	21	theme	maximum	578:584	arg1	423 mg/g					599:606	423 mg/g	599:606	423 mg/g	599:606	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	2	21	theme	maximum	578:584	arg1	yield					592:596	the maximum sugar yield	574:596	the maximum sugar yield (423 mg/g)	574:607	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	1	22	theme	enzymatic	428:436	arg1	saccharification					438:453	enzymatic saccharification	428:453	enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL)	428:514	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	22	theme	enzymatic	428:436	arg1	loading					463:469	enzyme loading	456:469	enzyme loading: 2.5-17.5%, and reaction volume	456:501	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	23	dep	loading	463:469	arg1	%					480:480	2.5-17.5%	472:480	2.5-17.5%	472:480	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	23	dep	loading	463:469	arg1	550-850 µL					504:513	550-850 µL	504:513	550-850 µL	504:513	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	23	dep	loading	463:469	arg1	volume					496:501	reaction volume	487:501	reaction volume	487:501	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	24	theme	Fractional	183:192	arg1	FFD					212:214	FFD	212:214	FFD	212:214	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	24	theme	Fractional	183:192	arg1	Design					204:209	a high-resolution Fractional Factorial Design	165:209	a high-resolution Fractional Factorial Design (FFD)	165:215	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	4	25	theme	lignocellulosic	1096:1110	arg1	biomasses					1112:1120	other lignocellulosic biomasses	1090:1120	other lignocellulosic biomasses	1090:1120	This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.					
33310387	3	26	dep	80	863:864	arg1	to					860:861	to	860:861	to	860:861	Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25-110%).					
33310387	1	27	theme	Factorial	194:202	arg1	FFD					212:214	FFD	212:214	FFD	212:214	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	27	theme	Factorial	194:202	arg1	Design					204:209	a high-resolution Fractional Factorial Design	165:209	a high-resolution Fractional Factorial Design (FFD)	165:215	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	28	theme	enzyme	456:461	arg1	saccharification					438:453	enzymatic saccharification	428:453	enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL)	428:514	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	28	theme	enzyme	456:461	arg1	loading					463:469	enzyme loading	456:469	enzyme loading: 2.5-17.5%, and reaction volume	456:501	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	4	29	theme	hydrolysis	980:989	arg1	efficiency					991:1000	enzymatic hydrolysis efficiency	970:1000	enzymatic hydrolysis efficiency of sugarcane bagasse	970:1021	This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.					
33310387	0	30	theme	experiments	10:20	arg1	Design					0:5	Design	0:5	Design of experiments	0:20	Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse.					
33310387	4	31	theme	other	1090:1094	arg1	biomasses					1112:1120	other lignocellulosic biomasses	1090:1120	other lignocellulosic biomasses	1090:1120	This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.					
33310387	4	32	theme	sugarcane	1005:1013	arg1	bagasse					1015:1021	sugarcane bagasse	1005:1021	sugarcane bagasse	1005:1021	This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.					
33310387	1	33	dep	NaOH	359:362	arg1	temperature					378:388	temperature	378:388	temperature	378:388	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	33	dep	NaOH	359:362	arg1	0.1-1 mol/L					365:375	0.1-1 mol/L	365:375	0.1-1 mol/L	365:375	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	33	dep	NaOH	359:362	arg1	20-80 min					413:421	20-80 min	413:421	20-80 min	413:421	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	33	dep	NaOH	359:362	arg1	100-220 °C					391:400	100-220 °C	391:400	100-220 °C	391:400	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	33	dep	NaOH	359:362	arg1	time					407:410	time	407:410	time	407:410	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	3	34	theme	compositional	759:771	arg1	analyses					773:780	Biomass compositional analyses	751:780	Biomass compositional analyses	751:780	Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25-110%).					
33310387	0	35	theme	pretreatment	54:65	arg1	saccharification					71:86	saccharification	71:86	saccharification for sugarcane bagasse	71:108	Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse.					
33310387	0	35	theme	pretreatment	54:65	arg1	optimization					29:40	optimization	29:40	optimization of alkaline pretreatment	29:65	Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse.					
33310387	0	36	theme	alkaline	45:52	arg1	pretreatment					54:65	alkaline pretreatment	45:65	alkaline pretreatment	45:65	Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse.					
33310387	1	37	theme	wide	308:311	arg1	range					313:317	a wide range	306:317	a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL)	306:514	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	2	38	from	202 °C	701:706	arg1	NaOH					693:696	0.25 mol/L NaOH	682:696	0.25 mol/L NaOH at 202 °C for 40 min	682:717	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	2	39	theme	enzymatic	633:641	arg1	hydrolysis					643:652	18 h enzymatic hydrolysis	628:652	18 h enzymatic hydrolysis	628:652	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	1	40	theme	sugar	127:131	arg1	release					133:139	the sugar release	123:139	the sugar release from sugarcane bagasse	123:162	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	2	41	theme	loading	741:747	arg1	%					729:729	12.5%	725:729	12.5% of enzyme loading	725:747	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	2	41	theme	loading	741:747	arg1	loading					741:747	enzyme loading	734:747	enzyme loading	734:747	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	2	42	theme	18	628:629	arg1	h					631:631	h	631:631	h	631:631	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	2	43	theme	h	631:631	arg1	hydrolysis					643:652	18 h enzymatic hydrolysis	628:652	18 h enzymatic hydrolysis	628:652	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	2	44	theme	enzyme	734:739	arg1	loading					741:747	enzyme loading	734:747	enzyme loading	734:747	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	3	45	dep	70	843:844	arg1	to					840:841	to	840:841	to	840:841	Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25-110%).					
33310387	2	46	theme	0.25 mol/L	682:691	arg1	NaOH					693:696	0.25 mol/L NaOH	682:696	0.25 mol/L NaOH at 202 °C for 40 min	682:717	A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading).					
33310387	4	47	theme	enzymatic	970:978	arg1	efficiency					991:1000	enzymatic hydrolysis efficiency	970:1000	enzymatic hydrolysis efficiency of sugarcane bagasse	970:1021	This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.					
33310387	1	48	theme	sugarcane	146:154	arg1	bagasse					156:162	sugarcane bagasse	146:162	sugarcane bagasse	146:162	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	49	theme	reaction	487:494	arg1	volume					496:501	reaction volume	487:501	reaction volume	487:501	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
33310387	1	50	theme	Central	237:243	arg1	design					272:277	a Central Composite Orthogonal (CCO) design	235:277	a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL)	235:514	To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL).					
34454230	1	0	theme	calcium	194:200	arg1	beads					211:215	calcium alginate beads	194:215	calcium alginate beads	194:215	In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production.					
34454230	5	1	theme	biological	820:829	arg1	activity					831:838	biological activity	820:838	biological activity	820:838	The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control.					
34454230	6	2	theme	potential	976:984	arg1	application					986:996	the potential application	972:996	the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum	972:1111	This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.					
34454230	1	3	theme	alginate	202:209	arg1	beads					211:215	calcium alginate beads	194:215	calcium alginate beads	194:215	In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production.					
34454230	3	4	theme	illumination	546:557	arg1	application					496:506	simultaneous application	483:506	simultaneous application of immobilization and blue wavelength illumination	483:557	Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control.					
34454230	3	5	theme	phycobiliproteins	581:597	arg1	content					599:605	the phycobiliproteins content	577:605	the phycobiliproteins content	577:605	Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control.					
34454230	2	6	dep	increased	376:384	arg1	compared					422:429	compared	422:429	compared to those in the control, respectively	422:467	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	6	7	theme	production	1084:1093	arg1	application					986:996	the potential application	972:996	the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum	972:1111	This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.					
34454230	2	8	dep	79 ± 3.4	395:402	arg1	to					392:393	to	392:393	to	392:393	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	3	9	theme	blue	530:533	arg1	illumination					546:557	blue wavelength illumination	530:557	blue wavelength illumination	530:557	Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control.					
34454230	3	10	theme	immobilization	511:524	arg1	application					496:506	simultaneous application	483:506	simultaneous application of immobilization and blue wavelength illumination	483:557	Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control.					
34454230	3	11	theme	simultaneous	483:494	arg1	application					496:506	simultaneous application	483:506	simultaneous application of immobilization and blue wavelength illumination	483:557	Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control.					
34454230	2	12	dep	biomass	287:293	arg1	The					278:280	The	278:280	The	278:280	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	5	13	theme	polymeric	861:869	arg1	substances					871:880	the extracellular polymeric substances	843:880	the extracellular polymeric substances produced through immobilization	843:912	The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control.					
34454230	2	14	theme	SPs	305:307	arg1	productivity					309:320	daily SPs productivity	299:320	daily SPs productivity	299:320	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	0	15	theme	immobilized	16:26	arg1	cultivation					28:38	Semi-continuous immobilized cultivation	0:38	Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides	0:92	Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production.					
34454230	2	16	theme	immobilized	351:361	arg1	culture					363:369	the calcium alginate bead immobilized culture	325:369	the calcium alginate bead immobilized culture	325:369	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	0	17	theme	Semi-continuous	0:14	arg1	cultivation					28:38	Semi-continuous immobilized cultivation	0:38	Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides	0:92	Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production.					
34454230	6	18	theme	high-efficiency	1064:1078	arg1	SPs					1080:1082	continuous and high-efficiency SPs	1049:1082	continuous and high-efficiency SPs	1049:1082	This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.					
34454230	2	19	theme	bead	346:349	arg1	culture					363:369	the calcium alginate bead immobilized culture	325:369	the calcium alginate bead immobilized culture	325:369	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	2	20	from	biomass	287:293	arg1	culture					363:369	the calcium alginate bead immobilized culture	325:369	the calcium alginate bead immobilized culture	325:369	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	5	21	theme	substances	871:880	arg1	composition					804:814	chemical composition	795:814	chemical composition	795:814	The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control.					
34454230	5	21	theme	substances	871:880	arg1	activity					831:838	biological activity	820:838	biological activity	820:838	The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control.					
34454230	2	22	theme	alginate	337:344	arg1	culture					363:369	the calcium alginate bead immobilized culture	325:369	the calcium alginate bead immobilized culture	325:369	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	4	23	from	deficiencies	674:685	arg1	combination					690:700	combination	690:700	combination with immobilization	690:720	Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control.					
34454230	1	24	theme	sulfated	235:242	arg1	polysaccharides					244:258	sulfated polysaccharides	235:258	sulfated polysaccharides (SPs) production	235:275	In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production.					
34454230	1	24	theme	sulfated	235:242	arg1	SPs					261:263	SPs	261:263	SPs	261:263	In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production.					
34454230	6	25	theme	continuous	1049:1058	arg1	SPs					1080:1082	continuous and high-efficiency SPs	1049:1082	continuous and high-efficiency SPs	1049:1082	This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.					
34454230	1	26	theme	semi-continuous	121:135	arg1	cultivation					149:159	semi-continuous immobilized cultivation	121:159	semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads	121:215	In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production.					
34454230	1	27	theme	polysaccharides	244:258	arg1	production					266:275	sulfated polysaccharides (SPs) production	235:275	sulfated polysaccharides (SPs) production	235:275	In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production.					
34454230	0	28	theme	cruentum	56:63	arg1	cultivation					28:38	Semi-continuous immobilized cultivation	0:38	Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides	0:92	Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production.					
34454230	4	29	theme	SPs	738:740	arg1	productivity					742:753	daily SPs productivity	732:753	daily SPs productivity	732:753	Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control.					
34454230	6	30	theme	immobilization	1030:1043	arg1	production					1084:1093	calcium alginate beads-based immobilization for continuous and high-efficiency SPs production	1001:1093	calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum	1001:1111	This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.					
34454230	5	31	dep	composition	804:814	arg1	The					791:793	The	791:793	The	791:793	The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control.					
34454230	0	32	theme	sulfated	69:76	arg1	polysaccharides					78:92	sulfated polysaccharides	69:92	sulfated polysaccharides	69:92	Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production.					
34454230	6	33	theme	beads-based	1018:1028	arg1	production					1084:1093	calcium alginate beads-based immobilization for continuous and high-efficiency SPs production	1001:1093	calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum	1001:1111	This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.					
34454230	4	34	with	combination	690:700	arg1	immobilization					707:720	immobilization	707:720	immobilization	707:720	Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control.					
34454230	1	35	theme	immobilized	137:147	arg1	cultivation					149:159	semi-continuous immobilized cultivation	121:159	semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads	121:215	In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production.					
34454230	5	36	theme	extracellular	847:859	arg1	substances					871:880	the extracellular polymeric substances	843:880	the extracellular polymeric substances produced through immobilization	843:912	The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control.					
34454230	6	37	theme	alginate	1009:1016	arg1	production					1084:1093	calcium alginate beads-based immobilization for continuous and high-efficiency SPs production	1001:1093	calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum	1001:1111	This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.					
34454230	3	38	theme	wavelength	535:544	arg1	illumination					546:557	blue wavelength illumination	530:557	blue wavelength illumination	530:557	Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control.					
34454230	2	39	from	productivity	309:320	arg1	culture					363:369	the calcium alginate bead immobilized culture	325:369	the calcium alginate bead immobilized culture	325:369	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	6	40	theme	calcium	1001:1007	arg1	production					1084:1093	calcium alginate beads-based immobilization for continuous and high-efficiency SPs production	1001:1093	calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum	1001:1111	This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.					
34454230	2	41	theme	daily	299:303	arg1	productivity					309:320	daily SPs productivity	299:320	daily SPs productivity	299:320	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	0	42	dep	production	94:103	arg1	cultivation					28:38	Semi-continuous immobilized cultivation	0:38	Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides	0:92	Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production.					
34454230	2	43	theme	calcium	329:335	arg1	culture					363:369	the calcium alginate bead immobilized culture	325:369	the calcium alginate bead immobilized culture	325:369	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	5	44	theme	chemical	795:802	arg1	composition					804:814	chemical composition	795:814	chemical composition	795:814	The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control.					
34454230	4	45	theme	nutrient	665:672	arg1	deficiencies					674:685	nutrient deficiencies	665:685	nutrient deficiencies in combination with immobilization	665:720	Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control.					
34454230	1	46	theme	cruentum	177:184	arg1	cultivation					149:159	semi-continuous immobilized cultivation	121:159	semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads	121:215	In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production.					
34454230	2	47	theme	cell	282:285	arg1	biomass					287:293	cell biomass	282:293	cell biomass	282:293	The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively.					
34454230	4	48	theme	daily	732:736	arg1	productivity					742:753	daily SPs productivity	732:753	daily SPs productivity	732:753	Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control.					
32747228	4	0	theme	3	758:758	arg1	%					759:759	%	759:759	%	759:759	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	1	1	with	films	234:238	arg1	incorporation					249:261	the incorporation	245:261	the incorporation of hemicelluloses (HC)	245:284	The aim of this study was to investigate the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC).					
32747228	3	2	theme	loading	473:479	arg1	efficiency					481:490	the increased HC loading efficiency	456:490	the increased HC loading efficiency (L)	456:494	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	2	theme	loading	473:479	arg1	L					493:493	L	493:493	L	493:493	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	3	theme	bond	609:612	arg1	interactions					614:625	the increased hydrogen bond interactions	586:625	the increased hydrogen bond interactions between CS and HC	586:643	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	4	with	films	525:529	arg1	content					553:559	the increased HC content	536:559	the increased HC content	536:559	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	4	5	theme	MPa	694:696	arg1	strength					669:676	the tensile strength	657:676	the tensile strength of 32.81 ± 4.97 MPa and elongation at break	657:720	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	3	6	theme	swelling	500:507	arg1	S					519:519	S	519:519	S	519:519	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	6	theme	swelling	500:507	arg1	property					509:516	swelling property	500:516	swelling property (S)	500:520	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	1	7	theme	hemicelluloses	266:279	arg1	incorporation					249:261	the incorporation	245:261	the incorporation of hemicelluloses (HC)	245:284	The aim of this study was to investigate the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC).					
32747228	0	8	theme	physicochemical	101:115	arg1	properties					117:126	physicochemical properties	101:126	physicochemical properties	101:126	Development of functional chitosan-based composite films incorporated with hemicelluloses: Effect on physicochemical properties.					
32747228	4	9	theme	%	759:759	arg1	GC					782:783	GC	782:783	GC	782:783	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	4	9	theme	%	759:759	arg1	HC					761:762	3% HC	758:762	3% HC	758:762	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	6	10	theme	growth	1007:1012	arg1	inhibition					1014:1023	the increased growth inhibition	993:1023	the increased growth inhibition	993:1023	Moreover, antibacterial testing indicated that all films exhibited the increased growth inhibition against E. coli and S. aureus as increasing TiO2 content.					
32747228	1	11	theme	study	145:149	arg1	aim					133:135	The aim	129:135	The aim of this study	129:149	The aim of this study was to investigate the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC).					
32747228	4	12	theme	%	737:737	arg1	Films					646:650	Films	646:650	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67%	646:737	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	6	13	theme	increased	997:1005	arg1	inhibition					1014:1023	the increased growth inhibition	993:1023	the increased growth inhibition	993:1023	Moreover, antibacterial testing indicated that all films exhibited the increased growth inhibition against E. coli and S. aureus as increasing TiO2 content.					
32747228	1	14	theme	physicochemical	174:188	arg1	properties					190:199	the physicochemical properties	170:199	the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC)	170:284	The aim of this study was to investigate the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC).					
32747228	4	15	theme	±	687:687	arg1	MPa					694:696	32.81 ± 4.97 MPa	681:696	32.81 ± 4.97 MPa	681:696	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	2	16	theme	properties	394:403	arg1	terms					356:360	terms	356:360	terms of structural and morphological properties	356:403	Films were obtained via direct immersion method and characterized in terms of structural and morphological properties by FT-IR, XRD, XPS, SEM and EDS.					
32747228	2	17	theme	direct	311:316	arg1	method					328:333	direct immersion method	311:333	direct immersion method	311:333	Films were obtained via direct immersion method and characterized in terms of structural and morphological properties by FT-IR, XRD, XPS, SEM and EDS.					
32747228	2	18	theme	morphological	380:392	arg1	properties					394:403	structural and morphological properties	365:403	structural and morphological properties	365:403	Films were obtained via direct immersion method and characterized in terms of structural and morphological properties by FT-IR, XRD, XPS, SEM and EDS.					
32747228	6	19	theme	TiO2	1069:1072	arg1	content					1074:1080	TiO2 content	1069:1080	TiO2 content	1069:1080	Moreover, antibacterial testing indicated that all films exhibited the increased growth inhibition against E. coli and S. aureus as increasing TiO2 content.					
32747228	5	20	theme	water	791:795	arg1	WVP					817:819	WVP	817:819	WVP	817:819	The water vapor permeability (WVP) of films developed with the increased HC and GC contents, while the oxygen permeability (OP) decreased.					
32747228	5	20	theme	water	791:795	arg1	permeability					803:814	The water vapor permeability	787:814	The water vapor permeability (WVP) of films	787:829	The water vapor permeability (WVP) of films developed with the increased HC and GC contents, while the oxygen permeability (OP) decreased.					
32747228	4	21	theme	32.81	681:685	arg1	MPa					694:696	32.81 ± 4.97 MPa	681:696	32.81 ± 4.97 MPa	681:696	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	0	22	theme	chitosan-based	26:39	arg1	films					51:55	functional chitosan-based composite films	15:55	functional chitosan-based composite films incorporated with hemicelluloses	15:88	Development of functional chitosan-based composite films incorporated with hemicelluloses: Effect on physicochemical properties.					
32747228	5	23	theme	vapor	797:801	arg1	WVP					817:819	WVP	817:819	WVP	817:819	The water vapor permeability (WVP) of films developed with the increased HC and GC contents, while the oxygen permeability (OP) decreased.					
32747228	5	23	theme	vapor	797:801	arg1	permeability					803:814	The water vapor permeability	787:814	The water vapor permeability (WVP) of films	787:829	The water vapor permeability (WVP) of films developed with the increased HC and GC contents, while the oxygen permeability (OP) decreased.					
32747228	0	24	theme	functional	15:24	arg1	films					51:55	functional chitosan-based composite films	15:55	functional chitosan-based composite films incorporated with hemicelluloses	15:88	Development of functional chitosan-based composite films incorporated with hemicelluloses: Effect on physicochemical properties.					
32747228	5	25	theme	oxygen	890:895	arg1	permeability					897:908	the oxygen permeability	886:908	the oxygen permeability (OP)	886:913	The water vapor permeability (WVP) of films developed with the increased HC and GC contents, while the oxygen permeability (OP) decreased.					
32747228	5	25	theme	oxygen	890:895	arg1	OP					911:912	OP	911:912	OP	911:912	The water vapor permeability (WVP) of films developed with the increased HC and GC contents, while the oxygen permeability (OP) decreased.					
32747228	4	26	theme	tensile	661:667	arg1	strength					669:676	the tensile strength	657:676	the tensile strength of 32.81 ± 4.97 MPa and elongation at break	657:720	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	0	27	theme	films	51:55	arg1	Development					0:10	Development	0:10	Development of functional chitosan-based composite films incorporated with hemicelluloses: Effect on physicochemical properties.	0:127	Development of functional chitosan-based composite films incorporated with hemicelluloses: Effect on physicochemical properties.					
32747228	0	28	from	Effect	91:96	arg1	properties					117:126	physicochemical properties	101:126	physicochemical properties	101:126	Development of functional chitosan-based composite films incorporated with hemicelluloses: Effect on physicochemical properties.					
32747228	5	29	theme	increased	850:858	arg1	HC					860:861	the increased HC	846:861	the increased HC	846:861	The water vapor permeability (WVP) of films developed with the increased HC and GC contents, while the oxygen permeability (OP) decreased.					
32747228	0	30	theme	composite	41:49	arg1	films					51:55	functional chitosan-based composite films	15:55	functional chitosan-based composite films incorporated with hemicelluloses	15:88	Development of functional chitosan-based composite films incorporated with hemicelluloses: Effect on physicochemical properties.					
32747228	3	31	theme	increased	540:548	arg1	content					553:559	the increased HC content	536:559	the increased HC content	536:559	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	1	32	theme	based	218:222	arg1	films					234:238	chitosan (CS) based composite films	204:238	chitosan (CS) based composite films with the incorporation of hemicelluloses (HC)	204:284	The aim of this study was to investigate the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC).					
32747228	4	33	theme	20	768:769	arg1	%					770:770	%	770:770	%	770:770	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	3	34	theme	films	525:529	arg1	S					519:519	S	519:519	S	519:519	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	34	theme	films	525:529	arg1	efficiency					481:490	the increased HC loading efficiency	456:490	the increased HC loading efficiency (L)	456:494	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	34	theme	films	525:529	arg1	property					509:516	swelling property	500:516	swelling property (S)	500:520	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	34	theme	films	525:529	arg1	L					493:493	L	493:493	L	493:493	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	35	theme	increased	460:468	arg1	efficiency					481:490	the increased HC loading efficiency	456:490	the increased HC loading efficiency (L)	456:494	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	35	theme	increased	460:468	arg1	L					493:493	L	493:493	L	493:493	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	36	theme	increased	590:598	arg1	interactions					614:625	the increased hydrogen bond interactions	586:625	the increased hydrogen bond interactions between CS and HC	586:643	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	5	37	theme	GC	867:868	arg1	contents					870:877	GC contents	867:877	GC contents	867:877	The water vapor permeability (WVP) of films developed with the increased HC and GC contents, while the oxygen permeability (OP) decreased.					
32747228	3	38	theme	HC	470:471	arg1	efficiency					481:490	the increased HC loading efficiency	456:490	the increased HC loading efficiency (L)	456:494	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	3	38	theme	HC	470:471	arg1	L					493:493	L	493:493	L	493:493	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	4	39	theme	elongation	702:711	arg1	strength					669:676	the tensile strength	657:676	the tensile strength of 32.81 ± 4.97 MPa and elongation at break	657:720	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	3	40	theme	hydrogen	600:607	arg1	interactions					614:625	the increased hydrogen bond interactions	586:625	the increased hydrogen bond interactions between CS and HC	586:643	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	5	41	theme	films	825:829	arg1	WVP					817:819	WVP	817:819	WVP	817:819	The water vapor permeability (WVP) of films developed with the increased HC and GC contents, while the oxygen permeability (OP) decreased.					
32747228	5	41	theme	films	825:829	arg1	permeability					803:814	The water vapor permeability	787:814	The water vapor permeability (WVP) of films	787:829	The water vapor permeability (WVP) of films developed with the increased HC and GC contents, while the oxygen permeability (OP) decreased.					
32747228	4	42	from	break	716:720	arg1	strength					669:676	the tensile strength	657:676	the tensile strength of 32.81 ± 4.97 MPa and elongation at break	657:720	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	6	43	theme	antibacterial	936:948	arg1	testing					950:956	antibacterial testing	936:956	antibacterial testing	936:956	Moreover, antibacterial testing indicated that all films exhibited the increased growth inhibition against E. coli and S. aureus as increasing TiO2 content.					
32747228	1	44	theme	composite	224:232	arg1	films					234:238	chitosan (CS) based composite films	204:238	chitosan (CS) based composite films with the incorporation of hemicelluloses (HC)	204:284	The aim of this study was to investigate the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC).					
32747228	4	45	theme	%	770:770	arg1	glycerol					772:779	20% glycerol	768:779	20% glycerol	768:779	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	1	46	dep	based	218:222	arg1	CS					214:215	CS	214:215	CS	214:215	The aim of this study was to investigate the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC).					
32747228	1	46	dep	based	218:222	arg1	chitosan					204:211	chitosan	204:211	chitosan (CS)	204:216	The aim of this study was to investigate the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC).					
32747228	2	47	theme	immersion	318:326	arg1	method					328:333	direct immersion method	311:333	direct immersion method	311:333	Films were obtained via direct immersion method and characterized in terms of structural and morphological properties by FT-IR, XRD, XPS, SEM and EDS.					
32747228	1	48	theme	films	234:238	arg1	properties					190:199	the physicochemical properties	170:199	the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC)	170:284	The aim of this study was to investigate the physicochemical properties of chitosan (CS) based composite films with the incorporation of hemicelluloses (HC).					
32747228	3	49	theme	HC	550:551	arg1	content					553:559	the increased HC content	536:559	the increased HC content	536:559	By combining with the increased HC loading efficiency (L) and swelling property (S) of films with the increased HC content to 5%, this study proved the increased hydrogen bond interactions between CS and HC.					
32747228	2	50	theme	structural	365:374	arg1	properties					394:403	structural and morphological properties	365:403	structural and morphological properties	365:403	Films were obtained via direct immersion method and characterized in terms of structural and morphological properties by FT-IR, XRD, XPS, SEM and EDS.					
32747228	4	51	with	Films	646:650	arg1	strength					669:676	the tensile strength	657:676	the tensile strength of 32.81 ± 4.97 MPa and elongation at break	657:720	Films with the tensile strength of 32.81 ± 4.97 MPa and elongation at break of 39.63 ± 4.67% were obtained with 3% HC and 20% glycerol (GC).					
32747228	0	52	dep	Development	0:10	arg1	Effect					91:96	Effect	91:96	Development of functional chitosan-based composite films incorporated with hemicelluloses: Effect on physicochemical properties.	0:127	Development of functional chitosan-based composite films incorporated with hemicelluloses: Effect on physicochemical properties.					
32630609	1	0	theme	depolymerized	348:360	arg1	waste					389:393	depolymerized lignocellulose biorefinery waste	348:393	depolymerized lignocellulose biorefinery waste	348:393	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	0	1	theme	Degree	70:75	arg1	Matter					38:43	Humic Matter	32:43	Humic Matter	32:43	Bio-Based Hydrogels Composed of Humic Matter and Pectins of Different Degree of Methyl-Esterification.					
32630609	0	1	theme	Degree	70:75	arg1	Pectins					49:55	Pectins	49:55	Pectins of Different Degree of Methyl-Esterification	49:100	Bio-Based Hydrogels Composed of Humic Matter and Pectins of Different Degree of Methyl-Esterification.					
32630609	1	2	theme	lignocellulose	362:375	arg1	waste					389:393	depolymerized lignocellulose biorefinery waste	348:393	depolymerized lignocellulose biorefinery waste	348:393	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	6	3	theme	careful	1362:1368	arg1	choice					1370:1375	a careful choice	1360:1375	a careful choice of the characteristics of their components	1360:1418	Our study suggests that the efficiency of novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops is related to a careful choice of the characteristics of their components.					
32630609	1	4	theme	natural	181:187	arg1	pectins					189:195	natural pectins	181:195	natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste	181:393	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	1	5	theme	biorefinery	377:387	arg1	waste					389:393	depolymerized lignocellulose biorefinery waste	348:393	depolymerized lignocellulose biorefinery waste	348:393	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	4	6	theme	HS	846:847	arg1	composition					831:841	the molecular composition	817:841	the molecular composition of HS and HULIS	817:857	Our results indicated that the release properties of hydrogels were influenced by the molecular composition of HS and HULIS and by the different degrees of methyl-esterification of pectins.					
32630609	4	7	theme	molecular	821:829	arg1	composition					831:841	the molecular composition	817:841	the molecular composition of HS and HULIS	817:857	Our results indicated that the release properties of hydrogels were influenced by the molecular composition of HS and HULIS and by the different degrees of methyl-esterification of pectins.					
32630609	0	8	theme	Methyl-Esterification	80:100	arg1	Degree					70:75	Different Degree	60:75	Different Degree of Methyl-Esterification	60:100	Bio-Based Hydrogels Composed of Humic Matter and Pectins of Different Degree of Methyl-Esterification.					
32630609	5	9	theme	ionic	1153:1157	arg1	interactions					1159:1170	ionic interactions	1153:1170	ionic interactions between phloroglucinol and the polysaccharidic chains	1153:1224	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	6	10	theme	hydrogels	1287:1295	arg1	related					1349:1355	related	1349:1355	related	1349:1355	Our study suggests that the efficiency of novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops is related to a careful choice of the characteristics of their components.					
32630609	6	10	theme	hydrogels	1287:1295	arg1	efficiency					1255:1264	the efficiency	1251:1264	the efficiency of novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops	1251:1344	Our study suggests that the efficiency of novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops is related to a careful choice of the characteristics of their components.					
32630609	1	11	theme	cow	291:293	arg1	compost					302:308	cow manure compost	291:308	cow manure compost	291:308	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	4	12	theme	hydrogels	788:796	arg1	properties					774:783	the release properties	762:783	the release properties of hydrogels	762:796	Our results indicated that the release properties of hydrogels were influenced by the molecular composition of HS and HULIS and by the different degrees of methyl-esterification of pectins.					
32630609	3	13	theme	controlled	588:597	arg1	systems					607:613	controlled release systems	588:613	controlled release systems	588:613	Their ability to work as controlled release systems was tested by following the release kinetics of a previously incorporated model phenolic compound, like phloroglucinol.					
32630609	2	14	theme	solid-state	432:442	arg1	spectroscopy					452:463	solid-state 13C-NMR spectroscopy	432:463	solid-state 13C-NMR spectroscopy	432:463	The hydrogels were characterized by solid-state 13C-NMR spectroscopy, scanning electron microscopy, spectroscopic magnetic resonance imaging and rheological analyses.					
32630609	4	15	theme	pectins	916:922	arg1	methyl-esterification					891:911	methyl-esterification	891:911	methyl-esterification of pectins	891:922	Our results indicated that the release properties of hydrogels were influenced by the molecular composition of HS and HULIS and by the different degrees of methyl-esterification of pectins.					
32630609	3	16	theme	release	599:605	arg1	systems					607:613	controlled release systems	588:613	controlled release systems	588:613	Their ability to work as controlled release systems was tested by following the release kinetics of a previously incorporated model phenolic compound, like phloroglucinol.					
32630609	2	17	theme	spectroscopic	496:508	arg1	imaging					529:535	spectroscopic magnetic resonance imaging	496:535	spectroscopic magnetic resonance imaging	496:535	The hydrogels were characterized by solid-state 13C-NMR spectroscopy, scanning electron microscopy, spectroscopic magnetic resonance imaging and rheological analyses.					
32630609	2	18	theme	rheological	541:551	arg1	analyses					553:560	rheological analyses	541:560	rheological analyses	541:560	The hydrogels were characterized by solid-state 13C-NMR spectroscopy, scanning electron microscopy, spectroscopic magnetic resonance imaging and rheological analyses.					
32630609	5	19	theme	interactions	1159:1170	arg1	formation					1140:1148	the least formation	1130:1148	the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains	1130:1224	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	1	20	theme	different	200:208	arg1	degree					210:215	different degree	200:215	different degree of methyl-esterification	200:240	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	0	21	theme	Bio-Based	0:8	arg1	Hydrogels					10:18	Bio-Based Hydrogels	0:18	Bio-Based Hydrogels	0:18	Bio-Based Hydrogels Composed of Humic Matter and Pectins of Different Degree of Methyl-Esterification.					
32630609	1	22	theme	manure	295:300	arg1	compost					302:308	cow manure compost	291:308	cow manure compost	291:308	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	5	23	theme	phloroglucinol	1005:1018	arg1	release					1020:1026	phloroglucinol release	1005:1026	phloroglucinol release	1005:1026	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	5	24	theme	polysaccharidic	1203:1217	arg1	chains					1219:1224	the polysaccharidic chains	1199:1224	the polysaccharidic chains	1199:1224	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	1	25	theme	degree	210:215	arg1	pectins					189:195	natural pectins	181:195	natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste	181:393	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	6	26	theme	humo-pectic	1275:1285	arg1	hydrogels					1287:1295	novel humo-pectic hydrogels	1269:1295	novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops	1269:1344	Our study suggests that the efficiency of novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops is related to a careful choice of the characteristics of their components.					
32630609	5	27	theme	crosslinking	1097:1108	arg1	density					1110:1116	crosslinking density	1097:1116	crosslinking density	1097:1116	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	5	28	theme	release	1020:1026	arg1	rate					997:1000	the fastest rate	985:1000	the fastest rate of phloroglucinol release	985:1026	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	6	29	theme	agroproducts	1324:1335	arg1	carriers					1312:1319	sustainable carriers	1300:1319	sustainable carriers of agroproducts to crops	1300:1344	Our study suggests that the efficiency of novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops is related to a careful choice of the characteristics of their components.					
32630609	2	30	theme	scanning	466:473	arg1	microscopy					484:493	scanning electron microscopy	466:493	scanning electron microscopy	466:493	The hydrogels were characterized by solid-state 13C-NMR spectroscopy, scanning electron microscopy, spectroscopic magnetic resonance imaging and rheological analyses.					
32630609	2	31	theme	electron	475:482	arg1	microscopy					484:493	scanning electron microscopy	466:493	scanning electron microscopy	466:493	The hydrogels were characterized by solid-state 13C-NMR spectroscopy, scanning electron microscopy, spectroscopic magnetic resonance imaging and rheological analyses.					
32630609	4	32	theme	HULIS	853:857	arg1	composition					831:841	the molecular composition	817:841	the molecular composition of HS and HULIS	817:857	Our results indicated that the release properties of hydrogels were influenced by the molecular composition of HS and HULIS and by the different degrees of methyl-esterification of pectins.					
32630609	4	33	theme	release	766:772	arg1	properties					774:783	the release properties	762:783	the release properties of hydrogels	762:796	Our results indicated that the release properties of hydrogels were influenced by the molecular composition of HS and HULIS and by the different degrees of methyl-esterification of pectins.					
32630609	1	34	theme	methyl-esterification	220:240	arg1	degree					210:215	different degree	200:215	different degree of methyl-esterification	200:240	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	1	35	theme	humic-like	313:322	arg1	HULIS					336:340	HULIS	336:340	HULIS	336:340	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	1	35	theme	humic-like	313:322	arg1	substances					324:333	humic-like substances	313:333	humic-like substances (HULIS)	313:341	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	6	36	theme	components	1409:1418	arg1	characteristics					1384:1398	the characteristics	1380:1398	the characteristics of their components	1380:1418	Our study suggests that the efficiency of novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops is related to a careful choice of the characteristics of their components.					
32630609	5	37	theme	least	1134:1138	arg1	formation					1140:1148	the least formation	1130:1148	the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains	1130:1224	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	5	38	theme	morphological	1069:1081	arg1	structure					1083:1091	its morphological structure	1065:1091	its morphological structure	1065:1091	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	5	39	theme	high	950:953	arg1	pectin					964:969	the high methoxyl pectin	946:969	the high methoxyl pectin	946:969	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	0	40	theme	Humic	32:36	arg1	Matter					38:43	Humic Matter	32:43	Humic Matter	32:43	Bio-Based Hydrogels Composed of Humic Matter and Pectins of Different Degree of Methyl-Esterification.					
32630609	3	41	theme	compound	704:711	arg1	kinetics					651:658	the release kinetics	639:658	the release kinetics of a previously incorporated model phenolic compound	639:711	Their ability to work as controlled release systems was tested by following the release kinetics of a previously incorporated model phenolic compound, like phloroglucinol.					
32630609	2	42	theme	13C-NMR	444:450	arg1	spectroscopy					452:463	solid-state 13C-NMR spectroscopy	432:463	solid-state 13C-NMR spectroscopy	432:463	The hydrogels were characterized by solid-state 13C-NMR spectroscopy, scanning electron microscopy, spectroscopic magnetic resonance imaging and rheological analyses.					
32630609	2	43	theme	resonance	519:527	arg1	imaging					529:535	spectroscopic magnetic resonance imaging	496:535	spectroscopic magnetic resonance imaging	496:535	The hydrogels were characterized by solid-state 13C-NMR spectroscopy, scanning electron microscopy, spectroscopic magnetic resonance imaging and rheological analyses.					
32630609	3	44	theme	release	643:649	arg1	kinetics					651:658	the release kinetics	639:658	the release kinetics of a previously incorporated model phenolic compound	639:711	Their ability to work as controlled release systems was tested by following the release kinetics of a previously incorporated model phenolic compound, like phloroglucinol.					
32630609	1	45	theme	humo-pectic	115:125	arg1	hydrogels					127:135	humo-pectic hydrogels	115:135	humo-pectic hydrogels	115:135	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	2	46	theme	magnetic	510:517	arg1	imaging					529:535	spectroscopic magnetic resonance imaging	496:535	spectroscopic magnetic resonance imaging	496:535	The hydrogels were characterized by solid-state 13C-NMR spectroscopy, scanning electron microscopy, spectroscopic magnetic resonance imaging and rheological analyses.					
32630609	6	47	theme	characteristics	1384:1398	arg1	choice					1370:1375	a careful choice	1360:1375	a careful choice of the characteristics of their components	1360:1418	Our study suggests that the efficiency of novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops is related to a careful choice of the characteristics of their components.					
32630609	4	48	theme	methyl-esterification	891:911	arg1	degrees					880:886	the different degrees	866:886	the different degrees of methyl-esterification of pectins	866:922	Our results indicated that the release properties of hydrogels were influenced by the molecular composition of HS and HULIS and by the different degrees of methyl-esterification of pectins.					
32630609	6	49	theme	sustainable	1300:1310	arg1	carriers					1312:1319	sustainable carriers	1300:1319	sustainable carriers of agroproducts to crops	1300:1344	Our study suggests that the efficiency of novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops is related to a careful choice of the characteristics of their components.					
32630609	6	50	theme	novel	1269:1273	arg1	hydrogels					1287:1295	novel humo-pectic hydrogels	1269:1295	novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops	1269:1344	Our study suggests that the efficiency of novel humo-pectic hydrogels as sustainable carriers of agroproducts to crops is related to a careful choice of the characteristics of their components.					
32630609	1	51	with	pectins	189:195	arg1	HS					272:273	HS	272:273	HS	272:273	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	1	51	with	pectins	189:195	arg1	substances					260:269	either humic substances	247:269	either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste	247:393	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	3	52	theme	model	689:693	arg1	compound					704:711	a previously incorporated model phenolic compound	663:711	a previously incorporated model phenolic compound	663:711	Their ability to work as controlled release systems was tested by following the release kinetics of a previously incorporated model phenolic compound, like phloroglucinol.					
32630609	5	53	theme	fastest	989:995	arg1	rate					997:1000	the fastest rate	985:1000	the fastest rate of phloroglucinol release	985:1026	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	1	54	theme	humic	254:258	arg1	HS					272:273	HS	272:273	HS	272:273	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	1	54	theme	humic	254:258	arg1	substances					260:269	either humic substances	247:269	either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste	247:393	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	3	55	theme	phenolic	695:702	arg1	compound					704:711	a previously incorporated model phenolic compound	663:711	a previously incorporated model phenolic compound	663:711	Their ability to work as controlled release systems was tested by following the release kinetics of a previously incorporated model phenolic compound, like phloroglucinol.					
32630609	1	56	theme	ionotropic	145:154	arg1	gelation					156:163	ionotropic gelation	145:163	ionotropic gelation	145:163	We prepared humo-pectic hydrogels through ionotropic gelation by crosslinking natural pectins of different degree of methyl-esterification with either humic substances (HS) extracted from cow manure compost or humic-like substances (HULIS) from depolymerized lignocellulose biorefinery waste.					
32630609	0	57	theme	Different	60:68	arg1	Degree					70:75	Different Degree	60:75	Different Degree of Methyl-Esterification	60:100	Bio-Based Hydrogels Composed of Humic Matter and Pectins of Different Degree of Methyl-Esterification.					
32630609	4	58	theme	different	870:878	arg1	degrees					880:886	the different degrees	866:886	the different degrees of methyl-esterification of pectins	866:922	Our results indicated that the release properties of hydrogels were influenced by the molecular composition of HS and HULIS and by the different degrees of methyl-esterification of pectins.					
32630609	5	59	theme	methoxyl	955:962	arg1	pectin					964:969	the high methoxyl pectin	946:969	the high methoxyl pectin	946:969	The hydrogel made by the high methoxyl pectin and HS showed the fastest rate of phloroglucinol release, and this was attributed not only to its morphological structure and crosslinking density but also to the least formation of ionic interactions between phloroglucinol and the polysaccharidic chains.					
32630609	3	60	theme	incorporated	676:687	arg1	compound					704:711	a previously incorporated model phenolic compound	663:711	a previously incorporated model phenolic compound	663:711	Their ability to work as controlled release systems was tested by following the release kinetics of a previously incorporated model phenolic compound, like phloroglucinol.					
34463203	5	0	attach	derived	885:891	arg2	cellulose					875:883	cellulose	875:883	cellulose derived from pistachio shells	875:913	CNCs were isolated by acid hydrolysis of cellulose derived from pistachio shells.					
34463203	5	0	attach	derived	885:891	arg1	shells					908:913	pistachio shells	898:913	pistachio shells	898:913	CNCs were isolated by acid hydrolysis of cellulose derived from pistachio shells.					
34463203	2	1	theme	material	324:331	arg1	type					316:319	the type	312:319	the type of material	312:331	The nature, location and type of tissue to be regenerated determines the type of material to be used.					
34463203	3	2	contain	has	362:364	arg2	requirements					374:385	its own requirements	366:385	its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response	366:608	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	3	2	contain	has	362:364	arg1	construct					352:360	A bone construct	345:360	A bone construct	345:360	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	3	2	contain	has	362:364	arg2	osteoconductivity					395:411	osteoconductivity	395:411	osteoconductivity	395:411	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	8	3	theme	apatite	1335:1341	arg1	ratio					1427:1431	the stoichiometric ratio	1408:1431	the stoichiometric ratio of natural bone apatite	1408:1455	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	8	3	theme	apatite	1335:1341	arg1	crystals					1343:1350	apatite crystals	1335:1350	apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite	1335:1455	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	11	4	with	Samples	1834:1840	arg1	concentration					1854:1866	5% CNC concentration	1847:1866	5% CNC concentration	1847:1866	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	4	5	theme	engineering	814:824	arg1	matrix					826:831	bone tissue engineering matrix	802:831	bone tissue engineering matrix	802:831	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	6	6	theme	X-RAY	1120:1124	arg1	analysis					1138:1145	X-RAY Diffraction analysis	1120:1145	X-RAY Diffraction analysis (XRD)	1120:1151	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	6	6	theme	X-RAY	1120:1124	arg1	XRD					1148:1150	XRD	1148:1150	XRD	1148:1150	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	4	7	theme	alcohol	672:678	arg1	composite					753:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite	653:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process	653:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	3	8	theme	combination	552:562	arg1	search					529:534	search	529:534	search of new possible combination of materials aimed to improve tissue response	529:608	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	11	9	theme	differentiation	1896:1910	arg1	capabilities					1912:1923	highest cell differentiation capabilities	1883:1923	highest cell differentiation capabilities	1883:1923	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	4	10	theme	present	615:621	arg1	study					623:627	The present study	611:627	The present study	611:627	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	3	11	theme	new	539:541	arg1	combination					552:562	new possible combination	539:562	new possible combination of materials aimed to improve tissue response	539:608	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	1	12	theme	myriad	187:192	arg1	search					175:180	the search	171:180	the search of a myriad of polymer composites with desirable properties	171:240	The need for an ideal tissue construct has lead to the search of a myriad of polymer composites with desirable properties.					
34463203	1	13	theme	polymer	197:203	arg1	composites					205:214	polymer composites	197:214	polymer composites	197:214	The need for an ideal tissue construct has lead to the search of a myriad of polymer composites with desirable properties.					
34463203	6	14	theme	prepared	920:927	arg1	samples					938:944	The prepared scaffold samples	916:944	The prepared scaffold samples	916:944	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	10	15	theme	cell	1573:1576	arg1	viability					1578:1586	The highest cell viability	1561:1586	The highest cell viability	1561:1586	The highest cell viability was recorded in scaffolds with 5% CNC content.					
34463203	11	16	theme	higher	1751:1756	arg1	densities					1763:1771	higher cell densities	1751:1771	higher cell densities	1751:1771	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	3	17	theme	materials	567:575	arg1	combination					552:562	new possible combination	539:562	new possible combination of materials aimed to improve tissue response	539:608	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	8	18	theme	stoichiometric	1412:1425	arg1	ratio					1427:1431	the stoichiometric ratio	1408:1431	the stoichiometric ratio of natural bone apatite	1408:1455	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	8	18	theme	stoichiometric	1412:1425	arg1	crystals					1343:1350	apatite crystals	1335:1350	apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite	1335:1455	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	1	19	theme	ideal	136:140	arg1	construct					149:157	an ideal tissue construct	133:157	an ideal tissue construct	133:157	The need for an ideal tissue construct has lead to the search of a myriad of polymer composites with desirable properties.					
34463203	8	20	theme	%	1293:1293	arg1	concentration					1306:1318	1% and 5% CNC concentration	1292:1318	1% and 5% CNC concentration	1292:1318	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	7	21	theme	pore	1192:1195	arg1	morphology					1197:1206	pore morphology	1192:1206	pore morphology	1192:1206	The scaffolds exhibited refinement in pore morphology and increased mineralization tendency on increasing CNC concentration.					
34463203	11	22	dep	%	1724:1724	arg1	5					1716:1716	5	1716:1716	5	1716:1716	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	11	22	dep	%	1724:1724	arg1	10					1722:1723	10	1722:1723	10	1722:1723	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	3	23	theme	tissue	594:599	arg1	response					601:608	tissue response	594:608	tissue response	594:608	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	7	24	from	refinement	1178:1187	arg1	morphology					1197:1206	pore morphology	1192:1206	pore morphology	1192:1206	The scaffolds exhibited refinement in pore morphology and increased mineralization tendency on increasing CNC concentration.					
34463203	7	24	from	refinement	1178:1187	arg1	tendency					1237:1244	increased mineralization tendency	1212:1244	increased mineralization tendency	1212:1244	The scaffolds exhibited refinement in pore morphology and increased mineralization tendency on increasing CNC concentration.					
34463203	8	25	theme	bone	1444:1447	arg1	apatite					1449:1455	natural bone apatite	1436:1455	natural bone apatite	1436:1455	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	4	26	theme	/β-Tricalcium	685:697	arg1	composite					753:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite	653:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process	653:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	0	27	theme	bone	83:86	arg1	application					107:117	bone tissue engineering application	83:117	bone tissue engineering application	83:117	Effect of cellulose nanocrystals on chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application.					
34463203	11	28	dep	content	1730:1736	arg1	%					1724:1724	%	1724:1724	%	1724:1724	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	5	29	theme	cellulose	875:883	arg1	hydrolysis					861:870	acid hydrolysis	856:870	acid hydrolysis of cellulose derived from pistachio shells	856:913	CNCs were isolated by acid hydrolysis of cellulose derived from pistachio shells.					
34463203	0	30	from	Effect	0:5	arg1	scaffold					70:77	chitosan/PVA/nano β-TCP composite scaffold	36:77	chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application	36:117	Effect of cellulose nanocrystals on chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application.					
34463203	4	31	theme	porous	746:751	arg1	composite					753:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite	653:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process	653:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	0	32	theme	engineering	95:105	arg1	application					107:117	bone tissue engineering application	83:117	bone tissue engineering application	83:117	Effect of cellulose nanocrystals on chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application.					
34463203	6	33	theme	Fourier-transform	968:984	arg1	spectroscopy					995:1006	Fourier-transform infrared spectroscopy	968:1006	Fourier-transform infrared spectroscopy (FTIR)	968:1013	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	6	33	theme	Fourier-transform	968:984	arg1	FTIR					1009:1012	FTIR	1009:1012	FTIR	1009:1012	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	11	34	theme	highest	1883:1889	arg1	capabilities					1912:1923	highest cell differentiation capabilities	1883:1923	highest cell differentiation capabilities	1883:1923	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	10	35	theme	5	1619:1619	arg1	%					1620:1620	%	1620:1620	%	1620:1620	The highest cell viability was recorded in scaffolds with 5% CNC content.					
34463203	6	36	theme	Force	1057:1061	arg1	AFM					1075:1077	AFM	1075:1077	AFM	1075:1077	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	6	36	theme	Force	1057:1061	arg1	Microscopy					1063:1072	Atomic Force Microscopy	1050:1072	Atomic Force Microscopy (AFM)	1050:1078	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	3	37	theme	bone	347:350	arg1	construct					352:360	A bone construct	345:360	A bone construct	345:360	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	4	38	theme	nanocrystals	727:738	arg1	composite					753:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite	653:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process	653:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	4	39	theme	CNC	741:743	arg1	composite					753:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite	653:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process	653:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	0	40	theme	cellulose	10:18	arg1	nanocrystals					20:31	cellulose nanocrystals	10:31	cellulose nanocrystals	10:31	Effect of cellulose nanocrystals on chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application.					
34463203	1	41	theme	tissue	142:147	arg1	construct					149:157	an ideal tissue construct	133:157	an ideal tissue construct	133:157	The need for an ideal tissue construct has lead to the search of a myriad of polymer composites with desirable properties.					
34463203	3	42	theme	own	370:372	arg1	osteoconductivity					395:411	osteoconductivity	395:411	osteoconductivity	395:411	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	3	42	theme	own	370:372	arg1	requirements					374:385	its own requirements	366:385	its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response	366:608	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	8	43	with	Samples	1279:1285	arg1	concentration					1306:1318	1% and 5% CNC concentration	1292:1318	1% and 5% CNC concentration	1292:1318	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	3	44	theme	mineralization	414:427	arg1	osteoconductivity					395:411	osteoconductivity	395:411	osteoconductivity	395:411	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	3	44	theme	mineralization	414:427	arg1	tendency					429:436	mineralization tendency	414:436	mineralization tendency	414:436	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	1	45	with	myriad	187:192	arg1	properties					231:240	desirable properties	221:240	desirable properties	221:240	The need for an ideal tissue construct has lead to the search of a myriad of polymer composites with desirable properties.					
34463203	11	46	theme	cell	1642:1645	arg1	attachment					1647:1656	cell attachment	1642:1656	cell attachment tendency	1642:1665	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	4	47	theme	bone	802:805	arg1	matrix					826:831	bone tissue engineering matrix	802:831	bone tissue engineering matrix	802:831	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	0	48	theme	β-TCP	54:58	arg1	scaffold					70:77	chitosan/PVA/nano β-TCP composite scaffold	36:77	chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application	36:117	Effect of cellulose nanocrystals on chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application.					
34463203	7	49	theme	CNC	1260:1262	arg1	concentration					1264:1276	CNC concentration	1260:1276	CNC concentration	1260:1276	The scaffolds exhibited refinement in pore morphology and increased mineralization tendency on increasing CNC concentration.					
34463203	8	50	theme	1.66	1380:1383	arg1	ratio					1362:1366	Ca/P ratio	1357:1366	Ca/P ratio of 1.61 and 1.66	1357:1383	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	3	51	theme	degradation	452:462	arg1	osteoconductivity					395:411	osteoconductivity	395:411	osteoconductivity	395:411	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	3	51	theme	degradation	452:462	arg1	rate					464:467	synchronized degradation rate	439:467	synchronized degradation rate	439:467	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	1	52	theme	composites	205:214	arg1	myriad					187:192	a myriad	185:192	a myriad of polymer composites with desirable properties	185:240	The need for an ideal tissue construct has lead to the search of a myriad of polymer composites with desirable properties.					
34463203	6	53	theme	Scanning	1081:1088	arg1	SEM					1111:1113	SEM	1111:1113	SEM	1111:1113	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	6	53	theme	Scanning	1081:1088	arg1	Microscopy					1099:1108	Scanning Electron Microscopy	1081:1108	Scanning Electron Microscopy (SEM)	1081:1114	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	11	54	theme	72 h.	1828:1832	arg1	Samples					1834:1840	72 h. Samples	1828:1840	72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities	1828:1923	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	1	55	theme	desirable	221:229	arg1	properties					231:240	desirable properties	221:240	desirable properties	221:240	The need for an ideal tissue construct has lead to the search of a myriad of polymer composites with desirable properties.					
34463203	10	56	with	scaffolds	1604:1612	arg1	content					1626:1632	5% CNC content	1619:1632	5% CNC content	1619:1632	The highest cell viability was recorded in scaffolds with 5% CNC content.					
34463203	7	57	theme	mineralization	1222:1235	arg1	tendency					1237:1244	increased mineralization tendency	1212:1244	increased mineralization tendency	1212:1244	The scaffolds exhibited refinement in pore morphology and increased mineralization tendency on increasing CNC concentration.					
34463203	8	58	theme	1.61	1371:1374	arg1	ratio					1362:1366	Ca/P ratio	1357:1366	Ca/P ratio of 1.61 and 1.66	1357:1383	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	8	59	theme	5	1299:1299	arg1	%					1300:1300	%	1300:1300	%	1300:1300	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	10	60	theme	CNC	1622:1624	arg1	content					1626:1632	5% CNC content	1619:1632	5% CNC content	1619:1632	The highest cell viability was recorded in scaffolds with 5% CNC content.					
34463203	4	61	theme	tissue	807:812	arg1	matrix					826:831	bone tissue engineering matrix	802:831	bone tissue engineering matrix	802:831	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	6	62	theme	Diffraction	1126:1136	arg1	analysis					1138:1145	X-RAY Diffraction analysis	1120:1145	X-RAY Diffraction analysis (XRD)	1120:1151	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	6	62	theme	Diffraction	1126:1136	arg1	XRD					1148:1150	XRD	1148:1150	XRD	1148:1150	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	10	63	theme	highest	1565:1571	arg1	viability					1578:1586	The highest cell viability	1561:1586	The highest cell viability	1561:1586	The highest cell viability was recorded in scaffolds with 5% CNC content.					
34463203	3	64	theme	possible	543:550	arg1	combination					552:562	new possible combination	539:562	new possible combination of materials aimed to improve tissue response	539:608	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	2	65	dep	nature	247:252	arg1	The					243:245	The	243:245	The	243:245	The nature, location and type of tissue to be regenerated determines the type of material to be used.					
34463203	9	66	theme	composite	1498:1506	arg1	modulus					1470:1476	Compressive modulus	1458:1476	Compressive modulus of CS/PVA/β-TCP/CNC composite	1458:1506	Compressive modulus of CS/PVA/β-TCP/CNC composite increased on increasing the CNC concentration to 5%.					
34463203	11	67	theme	CNC	1726:1728	arg1	content					1730:1736	5 and 10% CNC content	1716:1736	5 and 10% CNC content	1716:1736	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	4	68	theme	Chitosan/Polyvinyl	653:670	arg1	PVA					681:683	PVA	681:683	PVA	681:683	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	4	68	theme	Chitosan/Polyvinyl	653:670	arg1	alcohol					672:678	Chitosan/Polyvinyl alcohol	653:678	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process	653:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	6	69	theme	scaffold	929:936	arg1	samples					938:944	The prepared scaffold samples	916:944	The prepared scaffold samples	916:944	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	11	70	theme	cell	1891:1894	arg1	capabilities					1912:1923	highest cell differentiation capabilities	1883:1923	highest cell differentiation capabilities	1883:1923	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	4	71	theme	Phosphate	699:707	arg1	composite					753:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite	653:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process	653:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	11	72	theme	cell	1758:1761	arg1	densities					1763:1771	higher cell densities	1751:1771	higher cell densities	1751:1771	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	4	73	theme	drying	773:778	arg1	process					780:786	freeze drying process	766:786	freeze drying process	766:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	5	74	theme	pistachio	898:906	arg1	shells					908:913	pistachio shells	898:913	pistachio shells	898:913	CNCs were isolated by acid hydrolysis of cellulose derived from pistachio shells.					
34463203	6	75	theme	Thermogravimetric	1016:1032	arg1	TGA					1044:1046	TGA	1044:1046	TGA	1044:1046	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	6	75	theme	Thermogravimetric	1016:1032	arg1	analysis					1034:1041	Thermogravimetric analysis	1016:1041	Thermogravimetric analysis (TGA)	1016:1047	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	8	76	theme	natural	1436:1442	arg1	apatite					1449:1455	natural bone apatite	1436:1455	natural bone apatite	1436:1455	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	8	77	theme	CNC	1302:1304	arg1	concentration					1306:1318	1% and 5% CNC concentration	1292:1318	1% and 5% CNC concentration	1292:1318	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	4	78	theme	β-TCP	710:714	arg1	composite					753:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite	653:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process	653:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	11	79	theme	significant	1778:1788	arg1	depositions					1798:1808	significant calcium depositions	1778:1808	significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities	1778:1923	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	5	80	theme	acid	856:859	arg1	hydrolysis					861:870	acid hydrolysis	856:870	acid hydrolysis of cellulose derived from pistachio shells	856:913	CNCs were isolated by acid hydrolysis of cellulose derived from pistachio shells.					
34463203	8	81	theme	apatite	1449:1455	arg1	ratio					1427:1431	the stoichiometric ratio	1408:1431	the stoichiometric ratio of natural bone apatite	1408:1455	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	8	81	theme	apatite	1449:1455	arg1	crystals					1343:1350	apatite crystals	1335:1350	apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite	1335:1455	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	8	82	theme	1	1292:1292	arg1	%					1293:1293	%	1293:1293	%	1293:1293	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	0	83	theme	tissue	88:93	arg1	application					107:117	bone tissue engineering application	83:117	bone tissue engineering application	83:117	Effect of cellulose nanocrystals on chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application.					
34463203	11	84	with	samples	1703:1709	arg1	content					1730:1736	5 and 10% CNC content	1716:1736	5 and 10% CNC content	1716:1736	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	9	85	theme	CS/PVA/β-TCP/CNC	1481:1496	arg1	composite					1498:1506	CS/PVA/β-TCP/CNC composite	1481:1506	CS/PVA/β-TCP/CNC composite	1481:1506	Compressive modulus of CS/PVA/β-TCP/CNC composite increased on increasing the CNC concentration to 5%.					
34463203	8	86	with	crystals	1343:1350	arg1	ratio					1362:1366	Ca/P ratio	1357:1366	Ca/P ratio of 1.61 and 1.66	1357:1383	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	2	87	theme	tissue	276:281	arg1	type					268:271	type	268:271	type	268:271	The nature, location and type of tissue to be regenerated determines the type of material to be used.					
34463203	2	87	theme	tissue	276:281	arg1	location					255:262	location	255:262	location	255:262	The nature, location and type of tissue to be regenerated determines the type of material to be used.					
34463203	2	87	theme	tissue	276:281	arg1	nature					247:252	nature	247:252	nature	247:252	The nature, location and type of tissue to be regenerated determines the type of material to be used.					
34463203	6	88	theme	infrared	986:993	arg1	spectroscopy					995:1006	Fourier-transform infrared spectroscopy	968:1006	Fourier-transform infrared spectroscopy (FTIR)	968:1013	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	6	88	theme	infrared	986:993	arg1	FTIR					1009:1012	FTIR	1009:1012	FTIR	1009:1012	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	9	89	theme	Compressive	1458:1468	arg1	modulus					1470:1476	Compressive modulus	1458:1476	Compressive modulus of CS/PVA/β-TCP/CNC composite	1458:1506	Compressive modulus of CS/PVA/β-TCP/CNC composite increased on increasing the CNC concentration to 5%.					
34463203	8	90	theme	%	1300:1300	arg1	concentration					1306:1318	1% and 5% CNC concentration	1292:1318	1% and 5% CNC concentration	1292:1318	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	4	91	theme	composite	753:761	arg1	fabrication					638:648	fabrication	638:648	fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix	638:831	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	11	92	located	observed	1671:1678	arg1	samples					1687:1693	all samples	1683:1693	all samples	1683:1693	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	11	92	located	observed	1671:1678	arg2	tendency					1658:1665	cell attachment tendency	1642:1665	cell attachment tendency	1642:1665	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	11	93	theme	CNC	1850:1852	arg1	concentration					1854:1866	5% CNC concentration	1847:1866	5% CNC concentration	1847:1866	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	9	94	theme	CNC	1536:1538	arg1	concentration					1540:1552	the CNC concentration	1532:1552	the CNC concentration	1532:1552	Compressive modulus of CS/PVA/β-TCP/CNC composite increased on increasing the CNC concentration to 5%.					
34463203	6	95	theme	Atomic	1050:1055	arg1	AFM					1075:1077	AFM	1075:1077	AFM	1075:1077	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	6	95	theme	Atomic	1050:1055	arg1	Microscopy					1063:1072	Atomic Force Microscopy	1050:1072	Atomic Force Microscopy (AFM)	1050:1078	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	4	96	theme	/Cellulose	716:725	arg1	composite					753:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite	653:761	Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process	653:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	11	97	theme	5	1847:1847	arg1	%					1848:1848	%	1848:1848	%	1848:1848	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	0	98	theme	nanocrystals	20:31	arg1	Effect					0:5	Effect	0:5	Effect of cellulose nanocrystals on chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application.	0:118	Effect of cellulose nanocrystals on chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application.					
34463203	11	99	theme	attachment	1647:1656	arg1	tendency					1658:1665	cell attachment tendency	1642:1665	cell attachment tendency	1642:1665	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	3	100	theme	synchronized	439:450	arg1	osteoconductivity					395:411	osteoconductivity	395:411	osteoconductivity	395:411	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	3	100	theme	synchronized	439:450	arg1	rate					464:467	synchronized degradation rate	439:467	synchronized degradation rate	439:467	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	0	101	theme	chitosan/PVA/nano	36:52	arg1	scaffold					70:77	chitosan/PVA/nano β-TCP composite scaffold	36:77	chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application	36:117	Effect of cellulose nanocrystals on chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application.					
34463203	11	102	theme	%	1848:1848	arg1	concentration					1854:1866	5% CNC concentration	1847:1866	5% CNC concentration	1847:1866	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	8	103	theme	Ca/P	1357:1360	arg1	ratio					1362:1366	Ca/P ratio	1357:1366	Ca/P ratio of 1.61 and 1.66	1357:1383	Samples with 1% and 5% CNC concentration have deposited apatite crystals with Ca/P ratio of 1.61 and 1.66 which is very close to the stoichiometric ratio of natural bone apatite.					
34463203	10	104	theme	%	1620:1620	arg1	content					1626:1632	5% CNC content	1619:1632	5% CNC content	1619:1632	The highest cell viability was recorded in scaffolds with 5% CNC content.					
34463203	0	105	theme	composite	60:68	arg1	scaffold					70:77	chitosan/PVA/nano β-TCP composite scaffold	36:77	chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application	36:117	Effect of cellulose nanocrystals on chitosan/PVA/nano β-TCP composite scaffold for bone tissue engineering application.					
34463203	3	106	dep	osteoconductivity	395:411	arg1	etc					507:509	potential etc	497:509	potential etc	497:509	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	3	107	theme	potential	497:505	arg1	etc					507:509	potential etc	497:509	potential etc	497:509	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	3	108	theme	osteogenic	470:479	arg1	osteoconductivity					395:411	osteoconductivity	395:411	osteoconductivity	395:411	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	3	108	theme	osteogenic	470:479	arg1	differentiation					481:495	osteogenic differentiation	470:495	osteogenic differentiation	470:495	A bone construct has its own requirements such as osteoconductivity, mineralization tendency, synchronized degradation rate, osteogenic differentiation potential etc, which results in search of new possible combination of materials aimed to improve tissue response.					
34463203	6	109	theme	Electron	1090:1097	arg1	SEM					1111:1113	SEM	1111:1113	SEM	1111:1113	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	6	109	theme	Electron	1090:1097	arg1	Microscopy					1099:1108	Scanning Electron Microscopy	1081:1108	Scanning Electron Microscopy (SEM)	1081:1114	The prepared scaffold samples were characterized by Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA), Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-RAY Diffraction analysis (XRD).					
34463203	4	110	theme	freeze	766:771	arg1	process					780:786	freeze drying process	766:786	freeze drying process	766:786	The present study involves fabrication of Chitosan/Polyvinyl alcohol (PVA)/β-Tricalcium Phosphate (β-TCP)/Cellulose nanocrystals (CNC) porous composite by freeze drying process to be used as bone tissue engineering matrix.					
34463203	11	111	theme	calcium	1790:1796	arg1	depositions					1798:1808	significant calcium depositions	1778:1808	significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities	1778:1923	Though cell attachment tendency was observed in all samples but the samples with 5 and 10% CNC content demonstrated higher cell densities with significant calcium depositions when cultured for 72 h. Samples with 5% CNC concentration also possessed highest cell differentiation capabilities.					
34463203	7	112	theme	increased	1212:1220	arg1	tendency					1237:1244	increased mineralization tendency	1212:1244	increased mineralization tendency	1212:1244	The scaffolds exhibited refinement in pore morphology and increased mineralization tendency on increasing CNC concentration.					
31930591	10	0	theme	oligomeric	1665:1674	arg1	substrates					1676:1685	oligomeric substrates	1665:1685	oligomeric substrates	1665:1685	We also show that activity differences between acidic mammalian chitinase and chitotriosidase are reduced with insoluble substrate, suggesting that previously reported activity differences with oligomeric substrates may have been driven by differential substrate specificity.					
31930591	9	1	dep	oligomeric	1434:1443	arg1	substrates					1459:1468	substrates	1459:1468	substrates	1459:1468	In contrast, naturally occurring gain-of-function mutations gave similar results with oligomeric and insoluble substrates.					
31930591	7	2	theme	relevant	953:960	arg1	substrates					969:978	more biologically relevant chitin substrates	935:978	more biologically relevant chitin substrates	935:978	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	5	3	theme	acidic	694:699	arg1	variants					721:728	hyperactive acidic mammalian chitinase variants	682:728	hyperactive acidic mammalian chitinase variants	682:728	We initially set out to reverse this phenotype by engineering hyperactive acidic mammalian chitinase variants.					
31930591	3	4	from	degradation	417:427	arg1	lungs					466:470	mammalian lungs	456:470	mammalian lungs	456:470	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	10	5	theme	mammalian	1525:1533	arg1	chitinase					1535:1543	acidic mammalian chitinase	1518:1543	acidic mammalian chitinase	1518:1543	We also show that activity differences between acidic mammalian chitinase and chitotriosidase are reduced with insoluble substrate, suggesting that previously reported activity differences with oligomeric substrates may have been driven by differential substrate specificity.					
31930591	10	6	with	differences	1648:1658	arg1	substrates					1676:1685	oligomeric substrates	1665:1685	oligomeric substrates	1665:1685	We also show that activity differences between acidic mammalian chitinase and chitotriosidase are reduced with insoluble substrate, suggesting that previously reported activity differences with oligomeric substrates may have been driven by differential substrate specificity.					
31930591	0	7	from	Differences	0:10	arg1	insoluble					80:88	insoluble	80:88	insoluble	80:88	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	0	7	from	Differences	0:10	arg1	soluble					68:74	soluble	68:74	soluble	68:74	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	0	7	from	Differences	0:10	arg1	activity					32:39	the chitinolytic activity	15:39	the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates	15:99	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	2	8	theme	enzymatic	295:303	arg1	degradation					305:315	enzymatic degradation	295:315	enzymatic degradation	295:315	As such, the insoluble crystalline structure of chitin poses significant challenges for enzymatic degradation.					
31930591	6	9	with	approach	749:756	arg1	substrates					786:795	commercial fluorogenic substrates	763:795	commercial fluorogenic substrates	763:795	Using a screening approach with commercial fluorogenic substrates, we identified mutations with consistent increases in activity.					
31930591	4	10	theme	mammalian	493:501	arg1	chitinase					503:511	acidic mammalian chitinase	486:511	acidic mammalian chitinase	486:511	Mutations to acidic mammalian chitinase have been associated with asthma, and genetic deletion in mice increases morbidity and mortality with age.					
31930591	5	11	theme	hyperactive	682:692	arg1	variants					721:728	hyperactive acidic mammalian chitinase variants	682:728	hyperactive acidic mammalian chitinase variants	682:728	We initially set out to reverse this phenotype by engineering hyperactive acidic mammalian chitinase variants.					
31930591	6	12	theme	screening	739:747	arg1	approach					749:756	a screening approach	737:756	a screening approach with commercial fluorogenic substrates	737:795	Using a screening approach with commercial fluorogenic substrates, we identified mutations with consistent increases in activity.					
31930591	3	13	theme	glycosyl	359:366	arg1	hydrolase					368:376	a processive glycosyl hydrolase	346:376	a processive glycosyl hydrolase	346:376	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	3	13	theme	glycosyl	359:366	arg1	chitinase					335:343	Acidic mammalian chitinase	318:343	Acidic mammalian chitinase	318:343	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	4	14	theme	acidic	486:491	arg1	chitinase					503:511	acidic mammalian chitinase	486:511	acidic mammalian chitinase	486:511	Mutations to acidic mammalian chitinase have been associated with asthma, and genetic deletion in mice increases morbidity and mortality with age.					
31930591	7	15	theme	due	1165:1167	arg1	activity					1156:1163	activity	1156:1163	activity due to the addition or removal of a carbohydrate-binding domain	1156:1227	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	7	16	theme	chitinase	1017:1025	arg1	activity					1027:1034	chitinase activity	1017:1034	chitinase activity with insoluble chitin	1017:1056	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	8	17	theme	insoluble	1316:1324	arg1	substrates					1326:1335	insoluble substrates	1316:1335	insoluble substrates	1316:1335	We show that the activity increases from our directed evolution screen were lost when insoluble substrates were used.					
31930591	1	18	theme	structural	166:175	arg1	rigidity					177:184	structural rigidity	166:184	structural rigidity	166:184	Chitin is an abundant polysaccharide used by many organisms for structural rigidity and water repulsion.					
31930591	8	19	theme	evolution	1284:1292	arg1	screen					1294:1299	our directed evolution screen	1271:1299	our directed evolution screen	1271:1299	We show that the activity increases from our directed evolution screen were lost when insoluble substrates were used.					
31930591	11	20	theme	physiological	1799:1811	arg1	substrates					1813:1822	physiological substrates	1799:1822	physiological substrates	1799:1822	These results highlight the need for assays against physiological substrates when engineering metabolic enzymes, and provide a new one-pot assay that may prove to be broadly applicable to engineering glycosyl hydrolases.					
31930591	7	21	theme	carbohydrate-binding	1201:1220	arg1	domain					1222:1227	a carbohydrate-binding domain	1199:1227	a carbohydrate-binding domain	1199:1227	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	10	22	theme	acidic	1518:1523	arg1	chitinase					1535:1543	acidic mammalian chitinase	1518:1543	acidic mammalian chitinase	1518:1543	We also show that activity differences between acidic mammalian chitinase and chitotriosidase are reduced with insoluble substrate, suggesting that previously reported activity differences with oligomeric substrates may have been driven by differential substrate specificity.					
31930591	2	23	theme	significant	268:278	arg1	challenges					280:289	significant challenges	268:289	significant challenges for enzymatic degradation	268:315	As such, the insoluble crystalline structure of chitin poses significant challenges for enzymatic degradation.					
31930591	3	24	theme	mammalian	456:464	arg1	lungs					466:470	mammalian lungs	456:470	mammalian lungs	456:470	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	8	25	theme	activity	1247:1254	arg1	increases					1256:1264	the activity increases	1243:1264	the activity increases from our directed evolution screen	1243:1299	We show that the activity increases from our directed evolution screen were lost when insoluble substrates were used.					
31930591	0	26	from	activity	32:39	arg1	insoluble					80:88	insoluble	80:88	insoluble	80:88	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	0	26	from	activity	32:39	arg1	soluble					68:74	soluble	68:74	soluble	68:74	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	6	27	theme	consistent	827:836	arg1	increases					838:846	consistent increases	827:846	consistent increases in activity	827:858	Using a screening approach with commercial fluorogenic substrates, we identified mutations with consistent increases in activity.					
31930591	8	28	used	used	1342:1345	arg2	substrates					1326:1335	insoluble substrates	1316:1335	insoluble substrates	1316:1335	We show that the activity increases from our directed evolution screen were lost when insoluble substrates were used.					
31930591	5	29	theme	mammalian	701:709	arg1	variants					721:728	hyperactive acidic mammalian chitinase variants	682:728	hyperactive acidic mammalian chitinase variants	682:728	We initially set out to reverse this phenotype by engineering hyperactive acidic mammalian chitinase variants.					
31930591	3	30	theme	environmental	432:444	arg1	chitin					446:451	environmental chitin	432:451	environmental chitin	432:451	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	7	31	dep	addition	1176:1183	arg1	the					1172:1174	the	1172:1174	the	1172:1174	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	9	32	theme	similar	1413:1419	arg1	results					1421:1427	similar results	1413:1427	similar results	1413:1427	In contrast, naturally occurring gain-of-function mutations gave similar results with oligomeric and insoluble substrates.					
31930591	8	33	theme	directed	1275:1282	arg1	screen					1294:1299	our directed evolution screen	1271:1299	our directed evolution screen	1271:1299	We show that the activity increases from our directed evolution screen were lost when insoluble substrates were used.					
31930591	10	34	theme	insoluble	1582:1590	arg1	substrate					1592:1600	insoluble substrate	1582:1600	insoluble substrate	1582:1600	We also show that activity differences between acidic mammalian chitinase and chitotriosidase are reduced with insoluble substrate, suggesting that previously reported activity differences with oligomeric substrates may have been driven by differential substrate specificity.					
31930591	11	35	theme	metabolic	1841:1849	arg1	enzymes					1851:1857	metabolic enzymes	1841:1857	metabolic enzymes	1841:1857	These results highlight the need for assays against physiological substrates when engineering metabolic enzymes, and provide a new one-pot assay that may prove to be broadly applicable to engineering glycosyl hydrolases.					
31930591	3	36	theme	chitin	446:451	arg1	degradation					417:427	the degradation	413:427	the degradation of environmental chitin in mammalian lungs	413:470	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	0	37	theme	chitinolytic	19:30	arg1	activity					32:39	the chitinolytic activity	15:39	the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates	15:99	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	8	38	from	screen	1294:1299	arg1	increases					1256:1264	the activity increases	1243:1264	the activity increases from our directed evolution screen	1243:1299	We show that the activity increases from our directed evolution screen were lost when insoluble substrates were used.					
31930591	7	39	theme	new	994:996	arg1	assays					998:1003	new assays	994:1003	new assays	994:1003	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	0	40	dep	soluble	68:74	arg1	substrates					90:99	substrates	90:99	substrates	90:99	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	7	41	theme	activity	886:893	arg1	increases					895:903	the activity increases	882:903	the activity increases observed	882:912	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	7	41	theme	activity	886:893	arg1	consistent					919:928	consistent	919:928	consistent	919:928	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	10	42	theme	activity	1489:1496	arg1	differences					1498:1508	activity differences	1489:1508	activity differences between acidic mammalian chitinase and chitotriosidase	1489:1563	We also show that activity differences between acidic mammalian chitinase and chitotriosidase are reduced with insoluble substrate, suggesting that previously reported activity differences with oligomeric substrates may have been driven by differential substrate specificity.					
31930591	3	43	theme	primary	386:392	arg1	enzyme					394:399	the primary enzyme	382:399	the primary enzyme involved in the degradation of environmental chitin in mammalian lungs	382:470	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	3	43	theme	primary	386:392	arg1	chitinase					335:343	Acidic mammalian chitinase	318:343	Acidic mammalian chitinase	318:343	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	0	44	from	insoluble	80:88	arg1	Differences					0:10	Differences	0:10	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.	0:100	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	4	45	from	deletion	559:566	arg1	mice					571:574	mice	571:574	mice	571:574	Mutations to acidic mammalian chitinase have been associated with asthma, and genetic deletion in mice increases morbidity and mortality with age.					
31930591	11	46	theme	engineering	1935:1945	arg1	hydrolases					1956:1965	engineering glycosyl hydrolases	1935:1965	engineering glycosyl hydrolases	1935:1965	These results highlight the need for assays against physiological substrates when engineering metabolic enzymes, and provide a new one-pot assay that may prove to be broadly applicable to engineering glycosyl hydrolases.					
31930591	9	47	theme	gain-of-function	1381:1396	arg1	mutations					1398:1406	naturally occurring gain-of-function mutations	1361:1406	naturally occurring gain-of-function mutations	1361:1406	In contrast, naturally occurring gain-of-function mutations gave similar results with oligomeric and insoluble substrates.					
31930591	10	48	theme	differential	1711:1722	arg1	specificity					1734:1744	differential substrate specificity	1711:1744	differential substrate specificity	1711:1744	We also show that activity differences between acidic mammalian chitinase and chitotriosidase are reduced with insoluble substrate, suggesting that previously reported activity differences with oligomeric substrates may have been driven by differential substrate specificity.					
31930591	2	49	theme	crystalline	230:240	arg1	structure					242:250	the insoluble crystalline structure	216:250	the insoluble crystalline structure of chitin	216:260	As such, the insoluble crystalline structure of chitin poses significant challenges for enzymatic degradation.					
31930591	1	50	theme	water	190:194	arg1	repulsion					196:204	water repulsion	190:204	water repulsion	190:204	Chitin is an abundant polysaccharide used by many organisms for structural rigidity and water repulsion.					
31930591	6	51	from	increases	838:846	arg1	activity					851:858	activity	851:858	activity	851:858	Using a screening approach with commercial fluorogenic substrates, we identified mutations with consistent increases in activity.					
31930591	7	52	theme	domain	1222:1227	arg1	addition					1176:1183	addition	1176:1183	addition	1176:1183	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	7	52	theme	domain	1222:1227	arg1	removal					1188:1194	removal	1188:1194	removal	1188:1194	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	0	53	from	soluble	68:74	arg1	Differences					0:10	Differences	0:10	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.	0:100	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	2	54	theme	insoluble	220:228	arg1	structure					242:250	the insoluble crystalline structure	216:250	the insoluble crystalline structure of chitin	216:260	As such, the insoluble crystalline structure of chitin poses significant challenges for enzymatic degradation.					
31930591	7	55	theme	one-pot	1076:1082	arg1	assay					1096:1100	a one-pot fluorogenic assay	1074:1100	a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain	1074:1227	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	7	55	theme	one-pot	1076:1082	arg1	sensitive					1123:1131	sensitive	1123:1131	sensitive	1123:1131	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	11	56	theme	glycosyl	1947:1954	arg1	hydrolases					1956:1965	engineering glycosyl hydrolases	1935:1965	engineering glycosyl hydrolases	1935:1965	These results highlight the need for assays against physiological substrates when engineering metabolic enzymes, and provide a new one-pot assay that may prove to be broadly applicable to engineering glycosyl hydrolases.					
31930591	0	57	theme	chitinases	54:63	arg1	activity					32:39	the chitinolytic activity	15:39	the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates	15:99	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	3	58	theme	Acidic	318:323	arg1	enzyme					394:399	the primary enzyme	382:399	the primary enzyme involved in the degradation of environmental chitin in mammalian lungs	382:470	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	3	58	theme	Acidic	318:323	arg1	hydrolase					368:376	a processive glycosyl hydrolase	346:376	a processive glycosyl hydrolase	346:376	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	3	58	theme	Acidic	318:323	arg1	chitinase					335:343	Acidic mammalian chitinase	318:343	Acidic mammalian chitinase	318:343	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	7	59	theme	fluorogenic	1084:1094	arg1	assay					1096:1100	a one-pot fluorogenic assay	1074:1100	a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain	1074:1227	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	7	59	theme	fluorogenic	1084:1094	arg1	sensitive					1123:1131	sensitive	1123:1131	sensitive	1123:1131	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	10	60	theme	activity	1639:1646	arg1	differences					1648:1658	previously reported activity differences	1619:1658	previously reported activity differences with oligomeric substrates	1619:1685	We also show that activity differences between acidic mammalian chitinase and chitotriosidase are reduced with insoluble substrate, suggesting that previously reported activity differences with oligomeric substrates may have been driven by differential substrate specificity.					
31930591	0	61	theme	mammalian	44:52	arg1	chitinases					54:63	mammalian chitinases	44:63	mammalian chitinases	44:63	Differences in the chitinolytic activity of mammalian chitinases on soluble and insoluble substrates.					
31930591	7	62	with	activity	1027:1034	arg1	chitin					1051:1056	insoluble chitin	1041:1056	insoluble chitin	1041:1056	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	3	63	theme	mammalian	325:333	arg1	enzyme					394:399	the primary enzyme	382:399	the primary enzyme involved in the degradation of environmental chitin in mammalian lungs	382:470	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	3	63	theme	mammalian	325:333	arg1	hydrolase					368:376	a processive glycosyl hydrolase	346:376	a processive glycosyl hydrolase	346:376	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	3	63	theme	mammalian	325:333	arg1	chitinase					335:343	Acidic mammalian chitinase	318:343	Acidic mammalian chitinase	318:343	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	11	64	theme	new	1874:1876	arg1	assay					1886:1890	a new one-pot assay	1872:1890	a new one-pot assay that may prove to be broadly applicable to engineering glycosyl hydrolases	1872:1965	These results highlight the need for assays against physiological substrates when engineering metabolic enzymes, and provide a new one-pot assay that may prove to be broadly applicable to engineering glycosyl hydrolases.					
31930591	11	64	theme	new	1874:1876	arg1	applicable					1921:1930	applicable	1921:1930	applicable	1921:1930	These results highlight the need for assays against physiological substrates when engineering metabolic enzymes, and provide a new one-pot assay that may prove to be broadly applicable to engineering glycosyl hydrolases.					
31930591	1	65	theme	abundant	115:122	arg1	polysaccharide					124:137	an abundant polysaccharide	112:137	an abundant polysaccharide used by many organisms for structural rigidity and water repulsion	112:204	Chitin is an abundant polysaccharide used by many organisms for structural rigidity and water repulsion.					
31930591	1	65	theme	abundant	115:122	arg1	Chitin					102:107	Chitin	102:107	Chitin	102:107	Chitin is an abundant polysaccharide used by many organisms for structural rigidity and water repulsion.					
31930591	4	66	theme	genetic	551:557	arg1	deletion					559:566	genetic deletion	551:566	genetic deletion in mice	551:574	Mutations to acidic mammalian chitinase have been associated with asthma, and genetic deletion in mice increases morbidity and mortality with age.					
31930591	5	67	theme	chitinase	711:719	arg1	variants					721:728	hyperactive acidic mammalian chitinase variants	682:728	hyperactive acidic mammalian chitinase variants	682:728	We initially set out to reverse this phenotype by engineering hyperactive acidic mammalian chitinase variants.					
31930591	11	68	theme	one-pot	1878:1884	arg1	assay					1886:1890	a new one-pot assay	1872:1890	a new one-pot assay that may prove to be broadly applicable to engineering glycosyl hydrolases	1872:1965	These results highlight the need for assays against physiological substrates when engineering metabolic enzymes, and provide a new one-pot assay that may prove to be broadly applicable to engineering glycosyl hydrolases.					
31930591	11	68	theme	one-pot	1878:1884	arg1	applicable					1921:1930	applicable	1921:1930	applicable	1921:1930	These results highlight the need for assays against physiological substrates when engineering metabolic enzymes, and provide a new one-pot assay that may prove to be broadly applicable to engineering glycosyl hydrolases.					
31930591	2	69	theme	chitin	255:260	arg1	structure					242:250	the insoluble crystalline structure	216:250	the insoluble crystalline structure of chitin	216:260	As such, the insoluble crystalline structure of chitin poses significant challenges for enzymatic degradation.					
31930591	6	70	theme	fluorogenic	774:784	arg1	substrates					786:795	commercial fluorogenic substrates	763:795	commercial fluorogenic substrates	763:795	Using a screening approach with commercial fluorogenic substrates, we identified mutations with consistent increases in activity.					
31930591	10	71	theme	reported	1630:1637	arg1	differences					1648:1658	previously reported activity differences	1619:1658	previously reported activity differences with oligomeric substrates	1619:1685	We also show that activity differences between acidic mammalian chitinase and chitotriosidase are reduced with insoluble substrate, suggesting that previously reported activity differences with oligomeric substrates may have been driven by differential substrate specificity.					
31930591	7	72	theme	chitin	962:967	arg1	substrates					969:978	more biologically relevant chitin substrates	935:978	more biologically relevant chitin substrates	935:978	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	7	73	with	consistent	919:928	arg1	substrates					969:978	more biologically relevant chitin substrates	935:978	more biologically relevant chitin substrates	935:978	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	10	74	theme	substrate	1724:1732	arg1	specificity					1734:1744	differential substrate specificity	1711:1744	differential substrate specificity	1711:1744	We also show that activity differences between acidic mammalian chitinase and chitotriosidase are reduced with insoluble substrate, suggesting that previously reported activity differences with oligomeric substrates may have been driven by differential substrate specificity.					
31930591	6	75	theme	commercial	763:772	arg1	substrates					786:795	commercial fluorogenic substrates	763:795	commercial fluorogenic substrates	763:795	Using a screening approach with commercial fluorogenic substrates, we identified mutations with consistent increases in activity.					
31930591	3	76	theme	processive	348:357	arg1	hydrolase					368:376	a processive glycosyl hydrolase	346:376	a processive glycosyl hydrolase	346:376	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	3	76	theme	processive	348:357	arg1	chitinase					335:343	Acidic mammalian chitinase	318:343	Acidic mammalian chitinase	318:343	Acidic mammalian chitinase, a processive glycosyl hydrolase, is the primary enzyme involved in the degradation of environmental chitin in mammalian lungs.					
31930591	9	77	theme	occurring	1371:1379	arg1	mutations					1398:1406	naturally occurring gain-of-function mutations	1361:1406	naturally occurring gain-of-function mutations	1361:1406	In contrast, naturally occurring gain-of-function mutations gave similar results with oligomeric and insoluble substrates.					
31930591	7	78	theme	insoluble	1041:1049	arg1	chitin					1051:1056	insoluble chitin	1041:1056	insoluble chitin	1041:1056	To determine whether the activity increases observed were consistent with more biologically relevant chitin substrates, we developed new assays to quantify chitinase activity with insoluble chitin, and identified a one-pot fluorogenic assay that is sufficiently sensitive to quantify changes to activity due to the addition or removal of a carbohydrate-binding domain.					
31930591	1	79	theme	many	147:150	arg1	organisms					152:160	many organisms	147:160	many organisms	147:160	Chitin is an abundant polysaccharide used by many organisms for structural rigidity and water repulsion.					
33953365	0	0	theme	few	86:88	arg1	Flavobacteria					97:109	a few marine Flavobacteria	84:109	a few marine Flavobacteria	84:109	Isotopic tracing reveals single-cell assimilation of a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria.					
33953365	8	1	theme	polysaccharide-degrading	1550:1573	arg1	bacteria					1575:1582	polysaccharide-degrading bacteria	1550:1582	polysaccharide-degrading bacteria	1550:1582	These results highlight the high degree of niche specialization in heterotrophic communities and help constraining the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems.					
33953365	3	2	theme	bacterial	538:546	arg1	diversity					548:556	bacterial diversity	538:556	bacterial diversity	538:556	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	1	3	theme	oceanic	270:276	arg1	cycle					285:289	the oceanic carbon cycle	266:289	the oceanic carbon cycle	266:289	Algal polysaccharides constitute a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle.					
33953365	7	4	theme	Functional	1288:1297	arg1	screening					1299:1307	Functional screening	1288:1307	Functional screening of metagenomic libraries	1288:1332	Functional screening of metagenomic libraries gave insights into the genes of alginolytic Alteromonadales active in situ.					
33953365	1	5	theme	abundant	183:190	arg1	reservoir					192:200	a diverse and abundant reservoir	169:200	a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle	169:289	Algal polysaccharides constitute a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle.					
33953365	3	6	from	shift	585:589	arg1	diversity					548:556	bacterial diversity	538:556	bacterial diversity	538:556	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	1	7	theme	carbon	278:283	arg1	cycle					285:289	the oceanic carbon cycle	266:289	the oceanic carbon cycle	266:289	Algal polysaccharides constitute a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle.					
33953365	8	8	theme	quantitative	1529:1540	arg1	role					1542:1545	the quantitative role	1525:1545	the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems	1525:1604	These results highlight the high degree of niche specialization in heterotrophic communities and help constraining the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems.					
33953365	3	9	dep	induced	516:522	arg1	Congruent					436:444	Congruent	436:444	Congruent	436:444	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	8	10	theme	heterotrophic	1477:1489	arg1	communities					1491:1501	heterotrophic communities	1477:1501	heterotrophic communities	1477:1501	These results highlight the high degree of niche specialization in heterotrophic communities and help constraining the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems.					
33953365	0	11	theme	marine	90:95	arg1	Flavobacteria					97:109	a few marine Flavobacteria	84:109	a few marine Flavobacteria	84:109	Isotopic tracing reveals single-cell assimilation of a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria.					
33953365	5	12	dep	27.50 fg	1041:1048	arg1	to					1038:1039	to	1038:1039	to	1038:1039	Cell-specific alginate uptake was observed for Gammaproteobacteria and Flavobacteriales, with carbon assimilation rates ranging from 0.14 to 27.50 fg C µm-3 h-1.					
33953365	4	13	theme	ion	824:826	arg1	HISH-SIMS					847:855	HISH-SIMS	847:855	HISH-SIMS	847:855	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	4	13	theme	ion	824:826	arg1	spectrometry					833:844	nanoscale secondary ion mass spectrometry	804:844	nanoscale secondary ion mass spectrometry (HISH-SIMS)	804:856	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	3	14	with	Congruent	436:444	arg1	growth					456:461	cell growth	451:461	cell growth	451:461	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	3	14	with	Congruent	436:444	arg1	utilization					483:493	rapid substrate utilization	467:493	rapid substrate utilization	467:493	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	6	15	from	alginate	1175:1182	arg1	13C					1166:1168	13C	1166:1168	13C from alginate	1166:1182	DNA-SIP revealed that only a few initially rare Flavobacteriaceae and Alteromonadales taxa incorporated 13C from alginate into their biomass, accounting for most of the carbon assimilation based on bulk isotopic measurements.					
33953365	4	16	dep	in	771:772	arg1	situ					774:777	situ	774:777	situ	774:777	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	7	17	theme	alginolytic	1366:1376	arg1	Alteromonadales					1378:1392	alginolytic Alteromonadales	1366:1392	alginolytic Alteromonadales active in situ	1366:1407	Functional screening of metagenomic libraries gave insights into the genes of alginolytic Alteromonadales active in situ.					
33953365	3	18	theme	marked	564:569	arg1	shift					585:589	a marked compositional shift	562:589	a marked compositional shift towards copiotrophic bacteria	562:619	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	2	19	theme	kelp-dominated	403:416	arg1	habitats					426:433	kelp-dominated coastal habitats	403:433	kelp-dominated coastal habitats	403:433	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	3	20	theme	rapid	467:471	arg1	utilization					483:493	rapid substrate utilization	467:493	rapid substrate utilization	467:493	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	3	21	theme	compositional	571:583	arg1	shift					585:589	a marked compositional shift	562:589	a marked compositional shift towards copiotrophic bacteria	562:619	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	2	22	theme	coastal	418:424	arg1	habitats					426:433	kelp-dominated coastal habitats	403:433	kelp-dominated coastal habitats	403:433	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	4	23	theme	uptake	715:720	arg1	activity					722:729	the uptake activity	711:729	the uptake activity	711:729	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	6	24	theme	carbon	1231:1236	arg1	assimilation					1238:1249	the carbon assimilation	1227:1249	the carbon assimilation based on bulk isotopic measurements	1227:1285	DNA-SIP revealed that only a few initially rare Flavobacteriaceae and Alteromonadales taxa incorporated 13C from alginate into their biomass, accounting for most of the carbon assimilation based on bulk isotopic measurements.					
33953365	1	25	theme	organic	205:211	arg1	matter					213:218	organic matter	205:218	organic matter	205:218	Algal polysaccharides constitute a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle.					
33953365	0	26	theme	Isotopic	0:7	arg1	tracing					9:15	Isotopic tracing	0:15	Isotopic tracing	0:15	Isotopic tracing reveals single-cell assimilation of a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria.					
33953365	5	27	theme	µm-3	1052:1055	arg1	h-1					1057:1059	0.14 to 27.50 fg C µm-3 h-1	1033:1059	0.14 to 27.50 fg C µm-3 h-1	1033:1059	Cell-specific alginate uptake was observed for Gammaproteobacteria and Flavobacteriales, with carbon assimilation rates ranging from 0.14 to 27.50 fg C µm-3 h-1.					
33953365	3	28	from	decrease	526:533	arg1	diversity					548:556	bacterial diversity	538:556	bacterial diversity	538:556	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	8	29	theme	specialization	1459:1472	arg1	degree					1443:1448	the high degree	1434:1448	the high degree of niche specialization in heterotrophic communities	1434:1501	These results highlight the high degree of niche specialization in heterotrophic communities and help constraining the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems.					
33953365	8	30	theme	niche	1453:1457	arg1	specialization					1459:1472	niche specialization	1453:1472	niche specialization	1453:1472	These results highlight the high degree of niche specialization in heterotrophic communities and help constraining the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems.					
33953365	1	31	theme	matter	213:218	arg1	reservoir					192:200	a diverse and abundant reservoir	169:200	a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle	169:289	Algal polysaccharides constitute a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle.					
33953365	0	32	theme	single-cell	25:35	arg1	assimilation					37:48	single-cell assimilation	25:48	single-cell assimilation of a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria	25:133	Isotopic tracing reveals single-cell assimilation of a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria.					
33953365	4	33	theme	DNA	862:864	arg1	DNA-SIP					890:896	DNA-SIP	890:896	DNA-SIP	890:896	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	4	33	theme	DNA	862:864	arg1	probing					881:887	DNA stable isotope probing	862:887	DNA stable isotope probing (DNA-SIP)	862:897	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	8	34	theme	coastal	1587:1593	arg1	ecosystems					1595:1604	coastal ecosystems	1587:1604	coastal ecosystems	1587:1604	These results highlight the high degree of niche specialization in heterotrophic communities and help constraining the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems.					
33953365	5	35	theme	assimilation	1001:1012	arg1	rates					1014:1018	carbon assimilation rates	994:1018	carbon assimilation rates ranging from 0.14 to 27.50 fg C µm-3 h-1	994:1059	Cell-specific alginate uptake was observed for Gammaproteobacteria and Flavobacteriales, with carbon assimilation rates ranging from 0.14 to 27.50 fg C µm-3 h-1.					
33953365	2	36	theme	alginate	322:329	arg1	uptake					312:317	the uptake	308:317	the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats	308:433	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	3	37	theme	alginate	496:503	arg1	amendments					505:514	alginate amendments	496:514	alginate amendments	496:514	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	5	38	theme	C	1050:1050	arg1	h-1					1057:1059	0.14 to 27.50 fg C µm-3 h-1	1033:1059	0.14 to 27.50 fg C µm-3 h-1	1033:1059	Cell-specific alginate uptake was observed for Gammaproteobacteria and Flavobacteriales, with carbon assimilation rates ranging from 0.14 to 27.50 fg C µm-3 h-1.					
33953365	6	39	theme	bulk	1260:1263	arg1	measurements					1274:1285	bulk isotopic measurements	1260:1285	bulk isotopic measurements	1260:1285	DNA-SIP revealed that only a few initially rare Flavobacteriaceae and Alteromonadales taxa incorporated 13C from alginate into their biomass, accounting for most of the carbon assimilation based on bulk isotopic measurements.					
33953365	4	40	theme	nanoscale	804:812	arg1	HISH-SIMS					847:855	HISH-SIMS	847:855	HISH-SIMS	847:855	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	4	40	theme	nanoscale	804:812	arg1	spectrometry					833:844	nanoscale secondary ion mass spectrometry	804:844	nanoscale secondary ion mass spectrometry (HISH-SIMS)	804:856	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	8	41	from	role	1542:1545	arg1	ecosystems					1595:1604	coastal ecosystems	1587:1604	coastal ecosystems	1587:1604	These results highlight the high degree of niche specialization in heterotrophic communities and help constraining the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems.					
33953365	1	42	theme	marine	224:229	arg1	bacteria					245:252	marine heterotrophic bacteria	224:252	marine heterotrophic bacteria	224:252	Algal polysaccharides constitute a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle.					
33953365	2	43	theme	macroalgal	346:355	arg1	alginate					322:329	alginate	322:329	alginate	322:329	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	2	43	theme	macroalgal	346:355	arg1	polysaccharide					357:370	a major brown macroalgal polysaccharide	332:370	a major brown macroalgal polysaccharide	332:370	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	8	44	theme	bacteria	1575:1582	arg1	role					1542:1545	the quantitative role	1525:1545	the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems	1525:1604	These results highlight the high degree of niche specialization in heterotrophic communities and help constraining the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems.					
33953365	4	45	theme	isotope	873:879	arg1	DNA-SIP					890:896	DNA-SIP	890:896	DNA-SIP	890:896	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	4	45	theme	isotope	873:879	arg1	probing					881:887	DNA stable isotope probing	862:887	DNA stable isotope probing (DNA-SIP)	862:897	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	1	46	theme	heterotrophic	231:243	arg1	bacteria					245:252	marine heterotrophic bacteria	224:252	marine heterotrophic bacteria	224:252	Algal polysaccharides constitute a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle.					
33953365	3	47	theme	cell	451:454	arg1	growth					456:461	cell growth	451:461	cell growth	451:461	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	4	48	theme	stable	866:871	arg1	DNA-SIP					890:896	DNA-SIP	890:896	DNA-SIP	890:896	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	4	48	theme	stable	866:871	arg1	probing					881:887	DNA stable isotope probing	862:887	DNA stable isotope probing (DNA-SIP)	862:897	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	4	49	theme	secondary	814:822	arg1	HISH-SIMS					847:855	HISH-SIMS	847:855	HISH-SIMS	847:855	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	4	49	theme	secondary	814:822	arg1	spectrometry					833:844	nanoscale secondary ion mass spectrometry	804:844	nanoscale secondary ion mass spectrometry (HISH-SIMS)	804:856	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	0	50	theme	macroalgal	55:64	arg1	polysaccharide					66:79	a macroalgal polysaccharide	53:79	a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria	53:133	Isotopic tracing reveals single-cell assimilation of a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria.					
33953365	7	51	theme	libraries	1324:1332	arg1	screening					1299:1307	Functional screening	1288:1307	Functional screening of metagenomic libraries	1288:1332	Functional screening of metagenomic libraries gave insights into the genes of alginolytic Alteromonadales active in situ.					
33953365	4	52	theme	in	771:772	arg1	hybridization					779:791	halogen in situ hybridization	763:791	halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP)	763:897	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	7	53	dep	Alteromonadales	1378:1392	arg1	active					1394:1399	active	1394:1399	active	1394:1399	Functional screening of metagenomic libraries gave insights into the genes of alginolytic Alteromonadales active in situ.					
33953365	1	54	theme	diverse	171:177	arg1	reservoir					192:200	a diverse and abundant reservoir	169:200	a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle	169:289	Algal polysaccharides constitute a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle.					
33953365	2	55	from	habitats	426:433	arg1	communities					386:396	microbial communities	376:396	microbial communities from kelp-dominated coastal habitats	376:433	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	1	56	theme	Algal	136:140	arg1	polysaccharides					142:156	Algal polysaccharides	136:156	Algal polysaccharides	136:156	Algal polysaccharides constitute a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle.					
33953365	6	57	theme	isotopic	1265:1272	arg1	measurements					1274:1285	bulk isotopic measurements	1260:1285	bulk isotopic measurements	1260:1285	DNA-SIP revealed that only a few initially rare Flavobacteriaceae and Alteromonadales taxa incorporated 13C from alginate into their biomass, accounting for most of the carbon assimilation based on bulk isotopic measurements.					
33953365	4	58	theme	single-cell	738:748	arg1	level					750:754	the single-cell level	734:754	the single-cell level	734:754	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	2	59	theme	brown	340:344	arg1	alginate					322:329	alginate	322:329	alginate	322:329	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	2	59	theme	brown	340:344	arg1	polysaccharide					357:370	a major brown macroalgal polysaccharide	332:370	a major brown macroalgal polysaccharide	332:370	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	3	60	theme	copiotrophic	599:610	arg1	bacteria					612:619	copiotrophic bacteria	599:619	copiotrophic bacteria	599:619	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	5	61	theme	carbon	994:999	arg1	rates					1014:1018	carbon assimilation rates	994:1018	carbon assimilation rates ranging from 0.14 to 27.50 fg C µm-3 h-1	994:1059	Cell-specific alginate uptake was observed for Gammaproteobacteria and Flavobacteriales, with carbon assimilation rates ranging from 0.14 to 27.50 fg C µm-3 h-1.					
33953365	7	62	theme	Alteromonadales	1378:1392	arg1	genes					1357:1361	the genes	1353:1361	the genes of alginolytic Alteromonadales active in situ	1353:1407	Functional screening of metagenomic libraries gave insights into the genes of alginolytic Alteromonadales active in situ.					
33953365	4	63	theme	mass	828:831	arg1	HISH-SIMS					847:855	HISH-SIMS	847:855	HISH-SIMS	847:855	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	4	63	theme	mass	828:831	arg1	spectrometry					833:844	nanoscale secondary ion mass spectrometry	804:844	nanoscale secondary ion mass spectrometry (HISH-SIMS)	804:856	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	5	64	theme	alginate	914:921	arg1	uptake					923:928	Cell-specific alginate uptake	900:928	Cell-specific alginate uptake	900:928	Cell-specific alginate uptake was observed for Gammaproteobacteria and Flavobacteriales, with carbon assimilation rates ranging from 0.14 to 27.50 fg C µm-3 h-1.					
33953365	2	65	theme	major	334:338	arg1	alginate					322:329	alginate	322:329	alginate	322:329	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	2	65	theme	major	334:338	arg1	polysaccharide					357:370	a major brown macroalgal polysaccharide	332:370	a major brown macroalgal polysaccharide	332:370	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	8	66	theme	high	1438:1441	arg1	degree					1443:1448	the high degree	1434:1448	the high degree of niche specialization in heterotrophic communities	1434:1501	These results highlight the high degree of niche specialization in heterotrophic communities and help constraining the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems.					
33953365	1	67	theme	central	255:261	arg1	bacteria					245:252	marine heterotrophic bacteria	224:252	marine heterotrophic bacteria	224:252	Algal polysaccharides constitute a diverse and abundant reservoir of organic matter for marine heterotrophic bacteria, central to the oceanic carbon cycle.					
33953365	7	68	theme	metagenomic	1312:1322	arg1	libraries					1324:1332	metagenomic libraries	1312:1332	metagenomic libraries	1312:1332	Functional screening of metagenomic libraries gave insights into the genes of alginolytic Alteromonadales active in situ.					
33953365	5	69	theme	Cell-specific	900:912	arg1	uptake					923:928	Cell-specific alginate uptake	900:928	Cell-specific alginate uptake	900:928	Cell-specific alginate uptake was observed for Gammaproteobacteria and Flavobacteriales, with carbon assimilation rates ranging from 0.14 to 27.50 fg C µm-3 h-1.					
33953365	4	70	attach	derived	636:642	arg1	alginate					649:656	alginate	649:656	alginate	649:656	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	4	70	attach	derived	636:642	arg2	13C					632:634	13C	632:634	13C derived from alginate	632:656	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	3	71	theme	substrate	473:481	arg1	utilization					483:493	rapid substrate utilization	467:493	rapid substrate utilization	467:493	Congruent with cell growth and rapid substrate utilization, alginate amendments induced a decrease in bacterial diversity and a marked compositional shift towards copiotrophic bacteria.					
33953365	4	72	theme	bacterial	672:680	arg1	incorporators					682:694	specific bacterial incorporators	663:694	specific bacterial incorporators	663:694	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	0	73	theme	polysaccharide	66:79	arg1	assimilation					37:48	single-cell assimilation	25:48	single-cell assimilation of a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria	25:133	Isotopic tracing reveals single-cell assimilation of a macroalgal polysaccharide by a few marine Flavobacteria and Gammaproteobacteria.					
33953365	8	74	from	degree	1443:1448	arg1	communities					1491:1501	heterotrophic communities	1477:1501	heterotrophic communities	1477:1501	These results highlight the high degree of niche specialization in heterotrophic communities and help constraining the quantitative role of polysaccharide-degrading bacteria in coastal ecosystems.					
33953365	4	75	theme	specific	663:670	arg1	incorporators					682:694	specific bacterial incorporators	663:694	specific bacterial incorporators	663:694	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
33953365	2	76	theme	microbial	376:384	arg1	communities					386:396	microbial communities	376:396	microbial communities from kelp-dominated coastal habitats	376:433	We investigated the uptake of alginate, a major brown macroalgal polysaccharide, by microbial communities from kelp-dominated coastal habitats.					
33953365	4	77	theme	halogen	763:769	arg1	hybridization					779:791	halogen in situ hybridization	763:791	halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP)	763:897	We traced 13C derived from alginate into specific bacterial incorporators and quantified the uptake activity at the single-cell level, using halogen in situ hybridization coupled to nanoscale secondary ion mass spectrometry (HISH-SIMS) and DNA stable isotope probing (DNA-SIP).					
32709972	7	0	from	CAZy	1398:1401	arg1	proteome					1417:1424	microbiota proteome	1406:1424	microbiota proteome	1406:1424	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	7	1	theme	gut	1562:1564	arg1	microbes					1566:1573	gut microbes	1562:1573	gut microbes affecting their colonizing competence	1562:1611	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	3	2	theme	carbohydrate-active	546:564	arg1	enzymes					567:573	microbial CAZy (carbohydrate-active) enzymes	530:573	microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging	530:601	We profiled fecal microbiota in 63 healthy adult individuals using metaproteomics, and focused on microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging.					
32709972	4	3	theme	Bacteroides-derived	660:678	arg1	CAZy					680:683	many Bacteroides-derived CAZy	655:683	many Bacteroides-derived CAZy in more than one-third of subjects (n = 25)	655:727	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	6	4	theme	Prevotella	1286:1295	arg1	abundance					1273:1281	lower abundance	1267:1281	lower abundance of Prevotella	1267:1295	This associated invariably with high abundance of Prevotella in gut microbiota, while in subjects with lower abundance of Prevotella, microbiota showed no Prevotella-derived CAZy.					
32709972	3	5	theme	CAZy	540:543	arg1	enzymes					567:573	microbial CAZy (carbohydrate-active) enzymes	530:573	microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging	530:601	We profiled fecal microbiota in 63 healthy adult individuals using metaproteomics, and focused on microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging.					
32709972	1	6	theme	metabolic	175:183	arg1	functions					201:209	diverse metabolic and homeostatic functions	167:209	diverse metabolic and homeostatic functions related to health and well-being	167:242	Gut microbiota participates in diverse metabolic and homeostatic functions related to health and well-being.					
32709972	7	7	from	variation	1521:1529	arg1	specialization					1544:1557	metabolic specialization	1534:1557	metabolic specialization of gut microbes affecting their colonizing competence	1534:1611	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	7	8	link	Prevotella-derived	1379:1396	arg1	CAZy					1398:1401	Bacteroides- and Prevotella-derived CAZy	1362:1401	Bacteroides- and Prevotella-derived CAZy in microbiota proteome	1362:1424	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	6	9	theme	high	1196:1199	arg1	abundance					1201:1209	high abundance	1196:1209	high abundance of Prevotella in gut microbiota	1196:1241	This associated invariably with high abundance of Prevotella in gut microbiota, while in subjects with lower abundance of Prevotella, microbiota showed no Prevotella-derived CAZy.					
32709972	1	10	theme	homeostatic	189:199	arg1	functions					201:209	diverse metabolic and homeostatic functions	167:209	diverse metabolic and homeostatic functions related to health and well-being	167:242	Gut microbiota participates in diverse metabolic and homeostatic functions related to health and well-being.					
32709972	0	11	attach	links	44:48	arg2	profile					36:42	A carbohydrate-active enzyme (CAZy) profile	0:42	A carbohydrate-active enzyme (CAZy) profile	0:42	A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota.					
32709972	0	11	attach	links	44:48	arg1	abundance					107:115	its abundance	103:115	its abundance in gut microbiota	103:133	A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota.					
32709972	5	12	theme	glycosyl	1072:1079	arg1	families					1091:1098	glycosyl hydrolase families	1072:1098	glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides	1072:1161	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	1	13	theme	related	211:217	arg1	functions					201:209	diverse metabolic and homeostatic functions	167:209	diverse metabolic and homeostatic functions related to health and well-being	167:242	Gut microbiota participates in diverse metabolic and homeostatic functions related to health and well-being.					
32709972	6	14	theme	lower	1267:1271	arg1	abundance					1273:1281	lower abundance	1267:1281	lower abundance of Prevotella	1267:1295	This associated invariably with high abundance of Prevotella in gut microbiota, while in subjects with lower abundance of Prevotella, microbiota showed no Prevotella-derived CAZy.					
32709972	4	15	link	Bacteroides-derived	660:678	arg1	CAZy					680:683	many Bacteroides-derived CAZy	655:683	many Bacteroides-derived CAZy in more than one-third of subjects (n = 25)	655:727	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	5	16	theme	other	1048:1052	arg1	CAZy					1054:1057	several other CAZy	1040:1057	several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides	1040:1161	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	5	17	theme	intense	900:906	arg1	expression					908:917	intense expression	900:917	intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides	900:1161	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	0	18	theme	successful	50:59	arg1	specialization					71:84	successful metabolic specialization	50:84	successful metabolic specialization of Prevotella	50:98	A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota.					
32709972	5	19	theme	plant-derived	1133:1145	arg1	polysaccharides					1147:1161	complex plant-derived polysaccharides	1125:1161	complex plant-derived polysaccharides	1125:1161	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	5	20	theme	smaller	806:812	arg1	subset					814:819	a smaller subset	804:819	a smaller subset of donors (n = 8) with dietary parameters similar to others	804:879	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	5	21	theme	similar	863:869	arg1	parameters					852:861	dietary parameters	844:861	dietary parameters similar to others	844:879	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	4	22	theme	high	753:756	arg1	abundance					758:766	high abundance	753:766	high abundance of Bacteroides	753:781	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	0	23	theme	Prevotella	89:98	arg1	specialization					71:84	successful metabolic specialization	50:84	successful metabolic specialization of Prevotella	50:98	A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota.					
32709972	7	24	theme	individual	1510:1519	arg1	variation					1521:1529	individual variation	1510:1529	individual variation in metabolic specialization of gut microbes affecting their colonizing competence	1510:1611	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	5	25	theme	exo-beta-	956:964	arg1	-xylanase					970:978	exo-beta-(1,4)-xylanase	956:978	exo-beta-(1,4)-xylanase	956:978	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	3	26	theme	healthy	467:473	arg1	individuals					481:491	63 healthy adult individuals	464:491	63 healthy adult individuals using metaproteomics	464:512	We profiled fecal microbiota in 63 healthy adult individuals using metaproteomics, and focused on microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging.					
32709972	6	27	from	abundance	1201:1209	arg1	microbiota					1232:1241	gut microbiota	1228:1241	gut microbiota	1228:1241	This associated invariably with high abundance of Prevotella in gut microbiota, while in subjects with lower abundance of Prevotella, microbiota showed no Prevotella-derived CAZy.					
32709972	5	28	theme	1,4	966:968	arg1	-xylanase					970:978	exo-beta-(1,4)-xylanase	956:978	exo-beta-(1,4)-xylanase	956:978	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	7	29	from	proteome	1417:1424	arg1	Identification					1344:1357	Identification	1344:1357	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome	1344:1424	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	7	29	from	proteome	1417:1424	arg1	association					1436:1446	their association	1430:1446	their association with differences in microbiota composition	1430:1489	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	7	30	theme	CAZy	1398:1401	arg1	Identification					1344:1357	Identification	1344:1357	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome	1344:1424	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	7	30	theme	CAZy	1398:1401	arg1	association					1436:1446	their association	1430:1446	their association with differences in microbiota composition	1430:1489	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	5	31	with	donors	824:829	arg1	parameters					852:861	dietary parameters	844:861	dietary parameters similar to others	844:879	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	0	32	theme	enzyme	22:27	arg1	profile					36:42	A carbohydrate-active enzyme (CAZy) profile	0:42	A carbohydrate-active enzyme (CAZy) profile	0:42	A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota.					
32709972	5	33	theme	CAZy	941:944	arg1	expression					908:917	intense expression	900:917	intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides	900:1161	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	5	34	link	plant-derived	1133:1145	arg1	polysaccharides					1147:1161	complex plant-derived polysaccharides	1125:1161	complex plant-derived polysaccharides	1125:1161	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	0	35	theme	CAZy	30:33	arg1	profile					36:42	A carbohydrate-active enzyme (CAZy) profile	0:42	A carbohydrate-active enzyme (CAZy) profile	0:42	A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota.					
32709972	2	36	attach	present	404:410	arg2	nutrients					394:402	various nutrients	386:402	various nutrients present in gut environment	386:429	Its composition varies between individuals, and depends on factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment.					
32709972	2	36	attach	present	404:410	arg1	environment					419:429	gut environment	415:429	gut environment	415:429	Its composition varies between individuals, and depends on factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment.					
32709972	5	37	theme	several	1040:1046	arg1	CAZy					1054:1057	several other CAZy	1040:1057	several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides	1040:1161	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	7	38	from	Identification	1344:1357	arg1	proteome					1417:1424	microbiota proteome	1406:1424	microbiota proteome	1406:1424	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	7	39	theme	microbiota	1406:1415	arg1	proteome					1417:1424	microbiota proteome	1406:1424	microbiota proteome	1406:1424	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	3	40	theme	adult	475:479	arg1	individuals					481:491	63 healthy adult individuals	464:491	63 healthy adult individuals using metaproteomics	464:512	We profiled fecal microbiota in 63 healthy adult individuals using metaproteomics, and focused on microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging.					
32709972	7	41	theme	microbes	1566:1573	arg1	specialization					1544:1557	metabolic specialization	1534:1557	metabolic specialization of gut microbes affecting their colonizing competence	1534:1611	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	2	42	from	environment	419:429	arg1	present					404:410	present	404:410	present	404:410	Its composition varies between individuals, and depends on factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment.					
32709972	4	43	theme	distinct	622:629	arg1	one					646:648	one	646:648	one	646:648	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	4	43	theme	distinct	622:629	arg1	profiles					636:643	two distinct CAZy profiles	618:643	two distinct CAZy profiles	618:643	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	6	44	theme	Prevotella-derived	1319:1336	arg1	CAZy					1338:1341	no Prevotella-derived CAZy	1316:1341	no Prevotella-derived CAZy	1316:1341	This associated invariably with high abundance of Prevotella in gut microbiota, while in subjects with lower abundance of Prevotella, microbiota showed no Prevotella-derived CAZy.					
32709972	7	45	theme	metabolic	1534:1542	arg1	specialization					1544:1557	metabolic specialization	1534:1557	metabolic specialization of gut microbes affecting their colonizing competence	1534:1611	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	6	46	theme	Prevotella	1214:1223	arg1	abundance					1201:1209	high abundance	1196:1209	high abundance of Prevotella in gut microbiota	1196:1241	This associated invariably with high abundance of Prevotella in gut microbiota, while in subjects with lower abundance of Prevotella, microbiota showed no Prevotella-derived CAZy.					
32709972	3	47	theme	microbial	530:538	arg1	enzymes					567:573	microbial CAZy (carbohydrate-active) enzymes	530:573	microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging	530:601	We profiled fecal microbiota in 63 healthy adult individuals using metaproteomics, and focused on microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging.					
32709972	4	48	from	CAZy	680:683	arg1	one-third					698:706	one-third	698:706	one-third	698:706	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	4	48	from	CAZy	680:683	arg1	n = 25					721:726	n = 25	721:726	n = 25	721:726	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	4	48	from	CAZy	680:683	arg1	subjects					711:718	subjects	711:718	subjects (n = 25)	711:727	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	1	49	theme	diverse	167:173	arg1	functions					201:209	diverse metabolic and homeostatic functions	167:209	diverse metabolic and homeostatic functions related to health and well-being	167:242	Gut microbiota participates in diverse metabolic and homeostatic functions related to health and well-being.					
32709972	7	50	theme	variation	1521:1529	arg1	evidence					1498:1505	evidence	1498:1505	evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence	1498:1611	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	6	51	link	Prevotella-derived	1319:1336	arg1	CAZy					1338:1341	no Prevotella-derived CAZy	1316:1341	no Prevotella-derived CAZy	1316:1341	This associated invariably with high abundance of Prevotella in gut microbiota, while in subjects with lower abundance of Prevotella, microbiota showed no Prevotella-derived CAZy.					
32709972	4	52	theme	many	655:658	arg1	CAZy					680:683	many Bacteroides-derived CAZy	655:683	many Bacteroides-derived CAZy in more than one-third of subjects (n = 25)	655:727	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	0	53	theme	gut	120:122	arg1	microbiota					124:133	gut microbiota	120:133	gut microbiota	120:133	A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota.					
32709972	2	54	theme	related	312:318	arg1	factors					304:310	factors	304:310	factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment	304:429	Its composition varies between individuals, and depends on factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment.					
32709972	7	55	from	specialization	1544:1557	arg1	evidence					1498:1505	evidence	1498:1505	evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence	1498:1611	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	7	56	theme	microbiota	1468:1477	arg1	composition					1479:1489	microbiota composition	1468:1489	microbiota composition	1468:1489	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	7	57	with	Identification	1344:1357	arg1	differences					1453:1463	differences	1453:1463	differences in microbiota composition	1453:1489	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	5	58	theme	hydrolase	1081:1089	arg1	families					1091:1098	glycosyl hydrolase families	1072:1098	glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides	1072:1161	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	5	59	theme	dietary	844:850	arg1	parameters					852:861	dietary parameters	844:861	dietary parameters similar to others	844:879	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	5	60	theme	donors	824:829	arg1	subset					814:819	a smaller subset	804:819	a smaller subset of donors (n = 8) with dietary parameters similar to others	804:879	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	3	61	theme	glycan	587:592	arg1	foraging					594:601	glycan foraging	587:601	glycan foraging	587:601	We profiled fecal microbiota in 63 healthy adult individuals using metaproteomics, and focused on microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging.					
32709972	0	62	from	abundance	107:115	arg1	microbiota					124:133	gut microbiota	120:133	gut microbiota	120:133	A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota.					
32709972	4	63	theme	subjects	711:718	arg1	one-third					698:706	one-third	698:706	one-third	698:706	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	4	63	theme	subjects	711:718	arg1	n = 25					721:726	n = 25	721:726	n = 25	721:726	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	4	63	theme	subjects	711:718	arg1	subjects					711:718	subjects	711:718	subjects (n = 25)	711:727	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	6	64	with	subjects	1253:1260	arg1	abundance					1273:1281	lower abundance	1267:1281	lower abundance of Prevotella	1267:1295	This associated invariably with high abundance of Prevotella in gut microbiota, while in subjects with lower abundance of Prevotella, microbiota showed no Prevotella-derived CAZy.					
32709972	2	65	theme	microbial	332:340	arg1	communities					342:352	microbial communities	332:352	microbial communities	332:352	Its composition varies between individuals, and depends on factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment.					
32709972	0	66	theme	metabolic	61:69	arg1	specialization					71:84	successful metabolic specialization	50:84	successful metabolic specialization of Prevotella	50:98	A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota.					
32709972	1	67	theme	Gut	136:138	arg1	microbiota					140:149	Gut microbiota	136:149	Gut microbiota	136:149	Gut microbiota participates in diverse metabolic and homeostatic functions related to health and well-being.					
32709972	6	68	theme	gut	1228:1230	arg1	microbiota					1232:1241	gut microbiota	1228:1241	gut microbiota	1228:1241	This associated invariably with high abundance of Prevotella in gut microbiota, while in subjects with lower abundance of Prevotella, microbiota showed no Prevotella-derived CAZy.					
32709972	7	69	with	association	1436:1446	arg1	differences					1453:1463	differences	1453:1463	differences in microbiota composition	1453:1489	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	5	70	theme	polysaccharides	1147:1161	arg1	digestion					1112:1120	digestion	1112:1120	digestion of complex plant-derived polysaccharides	1112:1161	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	7	71	from	evidence	1498:1505	arg1	specialization					1544:1557	metabolic specialization	1534:1557	metabolic specialization of gut microbes affecting their colonizing competence	1534:1611	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	7	72	theme	Prevotella-derived	1379:1396	arg1	CAZy					1398:1401	Bacteroides- and Prevotella-derived CAZy	1362:1401	Bacteroides- and Prevotella-derived CAZy in microbiota proteome	1362:1424	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	2	73	theme	various	386:392	arg1	nutrients					394:402	various nutrients	386:402	various nutrients present in gut environment	386:429	Its composition varies between individuals, and depends on factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment.					
32709972	0	74	theme	carbohydrate-active	2:20	arg1	profile					36:42	A carbohydrate-active enzyme (CAZy) profile	0:42	A carbohydrate-active enzyme (CAZy) profile	0:42	A carbohydrate-active enzyme (CAZy) profile links successful metabolic specialization of Prevotella to its abundance in gut microbiota.					
32709972	5	75	theme	complex	1125:1131	arg1	polysaccharides					1147:1161	complex plant-derived polysaccharides	1125:1161	complex plant-derived polysaccharides	1125:1161	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	7	76	theme	Bacteroides-	1362:1373	arg1	CAZy					1398:1401	Bacteroides- and Prevotella-derived CAZy	1362:1401	Bacteroides- and Prevotella-derived CAZy in microbiota proteome	1362:1424	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	5	77	theme	Prevotella-derived	922:939	arg1	CAZy					941:944	Prevotella-derived CAZy	922:944	Prevotella-derived CAZy	922:944	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	7	78	from	association	1436:1446	arg1	proteome					1417:1424	microbiota proteome	1406:1424	microbiota proteome	1406:1424	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	2	79	theme	gut	415:417	arg1	environment					419:429	gut environment	415:429	gut environment	415:429	Its composition varies between individuals, and depends on factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment.					
32709972	7	80	from	differences	1453:1463	arg1	composition					1479:1489	microbiota composition	1468:1489	microbiota composition	1468:1489	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	3	81	from	microbiota	450:459	arg1	individuals					481:491	63 healthy adult individuals	464:491	63 healthy adult individuals using metaproteomics	464:512	We profiled fecal microbiota in 63 healthy adult individuals using metaproteomics, and focused on microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging.					
32709972	2	82	theme	present	404:410	arg1	nutrients					394:402	various nutrients	386:402	various nutrients present in gut environment	386:429	Its composition varies between individuals, and depends on factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment.					
32709972	4	83	theme	Bacteroides	771:781	arg1	abundance					758:766	high abundance	753:766	high abundance of Bacteroides	753:781	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	5	84	link	Prevotella-derived	922:939	arg1	CAZy					941:944	Prevotella-derived CAZy	922:944	Prevotella-derived CAZy	922:944	In a smaller subset of donors (n = 8) with dietary parameters similar to others, microbiota showed intense expression of Prevotella-derived CAZy including exo-beta-(1,4)-xylanase, xylan-1,4-beta-xylosidase, alpha-L-arabinofuranosidase and several other CAZy belonging to glycosyl hydrolase families involved in digestion of complex plant-derived polysaccharides.					
32709972	7	85	theme	colonizing	1591:1600	arg1	competence					1602:1611	their colonizing competence	1585:1611	their colonizing competence	1585:1611	Identification of Bacteroides- and Prevotella-derived CAZy in microbiota proteome and their association with differences in microbiota composition are in evidence of individual variation in metabolic specialization of gut microbes affecting their colonizing competence.					
32709972	2	86	from	present	404:410	arg1	environment					419:429	gut environment	415:429	gut environment	415:429	Its composition varies between individuals, and depends on factors related to host and microbial communities, which need to adapt to utilize various nutrients present in gut environment.					
32709972	3	87	theme	fecal	444:448	arg1	microbiota					450:459	fecal microbiota	444:459	fecal microbiota in 63 healthy adult individuals using metaproteomics	444:512	We profiled fecal microbiota in 63 healthy adult individuals using metaproteomics, and focused on microbial CAZy (carbohydrate-active) enzymes involved in glycan foraging.					
32709972	4	88	theme	CAZy	631:634	arg1	one					646:648	one	646:648	one	646:648	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	4	88	theme	CAZy	631:634	arg1	profiles					636:643	two distinct CAZy profiles	618:643	two distinct CAZy profiles	618:643	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32709972	4	89	theme	most	786:789	arg1	subjects					791:798	most subjects	786:798	most subjects	786:798	We identified two distinct CAZy profiles, one with many Bacteroides-derived CAZy in more than one-third of subjects (n = 25), and it associated with high abundance of Bacteroides in most subjects.					
32846181	1	0	from	mechanisms	171:180	arg1	rats					243:246	type 2 diabetic rats	227:246	type 2 diabetic rats	227:246	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	3	1	from	hyperglycemia	542:554	arg1	rats					636:639	rats	636:639	rats	636:639	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	1	2	theme	diabetic	234:241	arg1	rats					243:246	type 2 diabetic rats	227:246	type 2 diabetic rats	227:246	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	5	3	from	storage	1034:1040	arg1	liver					1045:1049	liver	1045:1049	liver	1045:1049	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	5	3	from	storage	1034:1040	arg1	muscle					1064:1069	skeletal muscle	1055:1069	skeletal muscle	1055:1069	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	1	4	theme	potential	147:155	arg1	mechanisms					171:180	the potential anti-diabetic mechanisms	143:180	the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats	143:246	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	5	5	theme	relative	1190:1197	arg1	expression					1199:1208	the relative expression	1186:1208	the relative expression of Egr-1 and PEPCK genes	1186:1233	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	4	6	theme	flora	894:898	arg1	modification					874:885	the modification	870:885	the modification of gut flora by HFP intervention	870:918	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	4	7	theme	diabetic	762:769	arg1	rats					771:774	diabetic rats	762:774	diabetic rats	762:774	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	4	8	theme	gut	890:892	arg1	flora					894:898	gut flora	890:898	gut flora	890:898	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	3	9	theme	insulin	557:563	arg1	resistance					565:574	insulin resistance	557:574	insulin resistance	557:574	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	3	10	from	stress	626:631	arg1	rats					636:639	rats	636:639	rats	636:639	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	0	11	from	consumption	58:68	arg1	diabetes					80:87	type 2 diabetes	73:87	type 2 diabetes	73:87	Mitigation mechanisms of Hizikia fusifarme polysaccharide consumption on type 2 diabetes in rats.					
32846181	0	11	from	consumption	58:68	arg1	rats					92:95	rats	92:95	rats	92:95	Mitigation mechanisms of Hizikia fusifarme polysaccharide consumption on type 2 diabetes in rats.					
32846181	3	12	theme	serum	473:477	arg1	profiles					479:486	serum profiles	473:486	serum profiles	473:486	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	2	13	theme	loading	266:272	arg1	experiment					274:283	The carbohydrate loading experiment	249:283	The carbohydrate loading experiment	249:283	The carbohydrate loading experiment illustrated that HFP supplement could reduce blood sugar fluctuations caused by eating through inhibiting the hydrolysis of starch in mice.					
32846181	4	14	from	composition	734:744	arg1	rats					771:774	diabetic rats	762:774	diabetic rats	762:774	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	1	15	theme	anti-diabetic	157:169	arg1	mechanisms					171:180	the potential anti-diabetic mechanisms	143:180	the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats	143:246	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	2	16	theme	carbohydrate	253:264	arg1	experiment					274:283	The carbohydrate loading experiment	249:283	The carbohydrate loading experiment	249:283	The carbohydrate loading experiment illustrated that HFP supplement could reduce blood sugar fluctuations caused by eating through inhibiting the hydrolysis of starch in mice.					
32846181	4	17	theme	sequencing	660:669	arg1	analysis					671:678	The 16s rRNA gene sequencing analysis	642:678	The 16s rRNA gene sequencing analysis	642:678	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	0	18	theme	Mitigation	0:9	arg1	mechanisms					11:20	Mitigation mechanisms	0:20	Mitigation mechanisms of Hizikia	0:31	Mitigation mechanisms of Hizikia fusifarme polysaccharide consumption on type 2 diabetes in rats.					
32846181	5	19	theme	PEPCK	1223:1227	arg1	genes					1229:1233	PEPCK genes	1223:1233	PEPCK genes	1223:1233	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	5	20	theme	rats	1083:1086	arg1	liver					1045:1049	liver	1045:1049	liver	1045:1049	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	5	20	theme	rats	1083:1086	arg1	muscle					1064:1069	skeletal muscle	1055:1069	skeletal muscle	1055:1069	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	4	21	theme	flora	753:757	arg1	composition					734:744	beneficial composition	723:744	beneficial composition of gut flora in diabetic rats	723:774	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	1	22	theme	Hizikia	185:191	arg1	polysaccharide					203:216	Hizikia fusifarme polysaccharide	185:216	Hizikia fusifarme polysaccharide (HFP)	185:222	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	1	22	theme	Hizikia	185:191	arg1	HFP					219:221	HFP	219:221	HFP	219:221	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	0	23	theme	Hizikia	25:31	arg1	mechanisms					11:20	Mitigation mechanisms	0:20	Mitigation mechanisms of Hizikia	0:31	Mitigation mechanisms of Hizikia fusifarme polysaccharide consumption on type 2 diabetes in rats.					
32846181	5	24	theme	genes	1229:1233	arg1	expression					1199:1208	the relative expression	1186:1208	the relative expression of Egr-1 and PEPCK genes	1186:1233	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	5	25	theme	signaling	1125:1133	arg1	pathway					1135:1141	IRS/PI3K/AKT/GLUT signaling pathway	1107:1141	IRS/PI3K/AKT/GLUT signaling pathway	1107:1141	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	5	26	theme	skeletal	1055:1062	arg1	muscle					1064:1069	skeletal muscle	1055:1069	skeletal muscle	1055:1069	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	2	27	theme	HFP	302:304	arg1	supplement					306:315	HFP supplement	302:315	HFP supplement	302:315	The carbohydrate loading experiment illustrated that HFP supplement could reduce blood sugar fluctuations caused by eating through inhibiting the hydrolysis of starch in mice.					
32846181	3	28	from	inflammation	599:610	arg1	rats					636:639	rats	636:639	rats	636:639	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	1	29	theme	fusifarme	193:201	arg1	polysaccharide					203:216	Hizikia fusifarme polysaccharide	185:216	Hizikia fusifarme polysaccharide (HFP)	185:222	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	1	29	theme	fusifarme	193:201	arg1	HFP					219:221	HFP	219:221	HFP	219:221	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	5	30	theme	western	950:956	arg1	analysis					967:974	the RT-qPCR and western blotting analysis	934:974	analysis	967:974	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	4	31	theme	correlation	785:795	arg1	analysis					797:804	the correlation analysis	781:804	the correlation analysis	781:804	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	3	32	theme	oral	500:503	arg1	administration					505:518	oral administration	500:518	oral administration of HFP	500:525	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	1	33	theme	polysaccharide	203:216	arg1	mechanisms					171:180	the potential anti-diabetic mechanisms	143:180	the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats	143:246	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	0	34	theme	polysaccharide	43:56	arg1	consumption					58:68	polysaccharide consumption	43:68	polysaccharide consumption on type 2 diabetes in rats	43:95	Mitigation mechanisms of Hizikia fusifarme polysaccharide consumption on type 2 diabetes in rats.					
32846181	2	35	theme	sugar	336:340	arg1	fluctuations					342:353	blood sugar fluctuations	330:353	blood sugar fluctuations caused by eating through inhibiting the hydrolysis of starch in mice	330:422	The carbohydrate loading experiment illustrated that HFP supplement could reduce blood sugar fluctuations caused by eating through inhibiting the hydrolysis of starch in mice.					
32846181	4	36	theme	gene	655:658	arg1	sequencing					660:669	The 16s rRNA gene sequencing	642:669	The 16s rRNA gene sequencing analysis	642:678	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	4	37	theme	rRNA	650:653	arg1	sequencing					660:669	The 16s rRNA gene sequencing	642:669	The 16s rRNA gene sequencing analysis	642:678	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	3	38	theme	diabetic	451:458	arg1	symptoms					460:467	typically diabetic symptoms	441:467	typically diabetic symptoms	441:467	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	5	39	theme	IRS/PI3K/AKT/GLUT	1107:1123	arg1	pathway					1135:1141	IRS/PI3K/AKT/GLUT signaling pathway	1107:1141	IRS/PI3K/AKT/GLUT signaling pathway	1107:1141	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	3	40	theme	oxidative	616:624	arg1	stress					626:631	oxidative stress	616:631	oxidative stress	616:631	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	2	41	theme	starch	409:414	arg1	hydrolysis					395:404	the hydrolysis	391:404	the hydrolysis of starch in mice	391:422	The carbohydrate loading experiment illustrated that HFP supplement could reduce blood sugar fluctuations caused by eating through inhibiting the hydrolysis of starch in mice.					
32846181	4	42	theme	HFP	903:905	arg1	intervention					907:918	HFP intervention	903:918	HFP intervention	903:918	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	5	43	theme	HFP	991:993	arg1	administration					995:1008	HFP administration	991:1008	HFP administration	991:1008	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	3	44	theme	chronic	591:597	arg1	inflammation					599:610	chronic inflammation	591:610	chronic inflammation	591:610	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	5	45	theme	Egr-1	1213:1217	arg1	expression					1199:1208	the relative expression	1186:1208	the relative expression of Egr-1 and PEPCK genes	1186:1233	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	3	46	theme	profiles	479:486	arg1	analysis					429:436	The analysis	425:436	The analysis of typically diabetic symptoms and serum profiles	425:486	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	4	47	theme	diabetes	839:846	arg1	related					859:865	related	859:865	related	859:865	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	4	47	theme	diabetes	839:846	arg1	improvement					824:834	the improvement	820:834	the improvement of diabetes	820:846	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	1	48	theme	current	115:121	arg1	work					123:126	current work	115:126	current work	115:126	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	3	49	from	resistance	565:574	arg1	rats					636:639	rats	636:639	rats	636:639	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	2	50	from	hydrolysis	395:404	arg1	mice					419:422	mice	419:422	mice	419:422	The carbohydrate loading experiment illustrated that HFP supplement could reduce blood sugar fluctuations caused by eating through inhibiting the hydrolysis of starch in mice.					
32846181	1	51	theme	work	123:126	arg1	objective					102:110	The objective	98:110	The objective of current work	98:126	The objective of current work was to explore the potential anti-diabetic mechanisms of Hizikia fusifarme polysaccharide (HFP) in type 2 diabetic rats.					
32846181	5	52	theme	blotting	958:965	arg1	analysis					967:974	the RT-qPCR and western blotting analysis	934:974	analysis	967:974	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	5	53	theme	diabetic	1074:1081	arg1	rats					1083:1086	diabetic rats	1074:1086	diabetic rats	1074:1086	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	4	54	theme	gut	749:751	arg1	flora					753:757	gut flora	749:757	gut flora	749:757	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	3	55	from	dyslipidemia	577:588	arg1	rats					636:639	rats	636:639	rats	636:639	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	2	56	theme	blood	330:334	arg1	fluctuations					342:353	blood sugar fluctuations	330:353	blood sugar fluctuations caused by eating through inhibiting the hydrolysis of starch in mice	330:422	The carbohydrate loading experiment illustrated that HFP supplement could reduce blood sugar fluctuations caused by eating through inhibiting the hydrolysis of starch in mice.					
32846181	3	57	theme	symptoms	460:467	arg1	analysis					429:436	The analysis	425:436	The analysis of typically diabetic symptoms and serum profiles	425:486	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	5	58	theme	glycogen	1025:1032	arg1	storage					1034:1040	glycogen storage	1025:1040	glycogen storage in liver and skeletal muscle of diabetic rats	1025:1086	Furthermore, the RT-qPCR and western blotting analysis clarified that HFP administration could increase glycogen storage in liver and skeletal muscle of diabetic rats through activating IRS/PI3K/AKT/GLUT signaling pathway and restrain gluconeogenesis via affecting the relative expression of Egr-1 and PEPCK genes.					
32846181	4	59	theme	16s	646:648	arg1	sequencing					660:669	The 16s rRNA gene sequencing	642:669	The 16s rRNA gene sequencing analysis	642:678	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	3	60	theme	HFP	523:525	arg1	administration					505:518	oral administration	500:518	oral administration of HFP	500:525	The analysis of typically diabetic symptoms and serum profiles showed that oral administration of HFP could mitigate hyperglycemia, insulin resistance, dyslipidemia, chronic inflammation and oxidative stress in rats.					
32846181	0	61	theme	type	73:76	arg1	diabetes					80:87	type 2 diabetes	73:87	type 2 diabetes	73:87	Mitigation mechanisms of Hizikia fusifarme polysaccharide consumption on type 2 diabetes in rats.					
32846181	4	62	theme	HFP	695:697	arg1	treatment					699:707	HFP treatment	695:707	HFP treatment	695:707	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32846181	4	63	theme	beneficial	723:732	arg1	composition					734:744	beneficial composition	723:744	beneficial composition of gut flora in diabetic rats	723:774	The 16s rRNA gene sequencing analysis indicated that HFP treatment could restore beneficial composition of gut flora in diabetic rats, and the correlation analysis revealed that the improvement of diabetes is closely related to the modification of gut flora by HFP intervention.					
32344634	1	0	theme	heat	313:316	arg1	treatment					318:326	saturated steam heat treatment	297:326	saturated steam heat treatment	297:326	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	5	1	theme	steam	788:792	arg1	treatment					799:807	the saturated steam heat treatment	774:807	the saturated steam heat treatment	774:807	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	4	2	theme	moisture	659:666	arg1	content					668:674	the initial moisture content	647:674	the initial moisture content of bamboo	647:684	With the increase in the initial moisture content of bamboo, the crystallinity of bamboo increased first and then decreased after treatment.					
32344634	5	3	from	content	927:933	arg1	bamboo					951:956	bamboo	951:956	bamboo with a 40% initial moisture content	951:992	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	2	4	theme	theoretical	442:452	arg1	basis					454:458	a theoretical basis	440:458	a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters	440:556	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	6	5	theme	shear	1034:1038	arg1	strength					1040:1047	The shear strength	1030:1047	The shear strength of treated bamboo	1030:1065	The shear strength of treated bamboo changed little within 10 min after saturated steam heat treatment, and then decreased rapidly.					
32344634	4	6	theme	initial	651:657	arg1	content					668:674	the initial moisture content	647:674	the initial moisture content of bamboo	647:684	With the increase in the initial moisture content of bamboo, the crystallinity of bamboo increased first and then decreased after treatment.					
32344634	1	7	theme	corresponding	214:226	arg1	composition					237:247	the corresponding chemical composition	210:247	the corresponding chemical composition	210:247	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	2	8	theme	properties	489:498	arg1	optimization					508:519	the optimization	504:519	the optimization of heat treatment	504:537	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	2	8	theme	properties	489:498	arg1	regulation					468:477	the regulation	464:477	the regulation of bamboo properties	464:498	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	0	9	from	Effect	0:5	arg1	Properties					66:75	Physical and Chemical Properties	44:75	Physical and Chemical Properties of Bamboo	44:85	Effect of Saturated Steam Heat Treatment on Physical and Chemical Properties of Bamboo.					
32344634	1	10	from	effects	133:139	arg1	properties					280:289	mechanical properties	269:289	mechanical properties	269:289	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	1	10	from	effects	133:139	arg1	crystallinity					250:262	crystallinity	250:262	crystallinity	250:262	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	1	10	from	effects	133:139	arg1	composition					237:247	the corresponding chemical composition	210:247	the corresponding chemical composition	210:247	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	6	11	theme	heat	1118:1121	arg1	treatment					1123:1131	saturated steam heat treatment	1102:1131	saturated steam heat treatment	1102:1131	The shear strength of treated bamboo changed little within 10 min after saturated steam heat treatment, and then decreased rapidly.					
32344634	5	12	theme	cellulose	938:946	arg1	content					927:933	the content	923:933	the content of cellulose in bamboo with a 40% initial moisture content	923:992	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	1	13	theme	chemical	228:235	arg1	composition					237:247	the corresponding chemical composition	210:247	the corresponding chemical composition	210:247	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	0	14	theme	Bamboo	80:85	arg1	Properties					66:75	Physical and Chemical Properties	44:75	Physical and Chemical Properties of Bamboo	44:85	Effect of Saturated Steam Heat Treatment on Physical and Chemical Properties of Bamboo.					
32344634	7	15	with	min	1182:1184	arg1	treatment					1212:1220	saturated steam heat treatment	1191:1220	saturated steam heat treatment	1191:1220	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	7	16	theme	first	1173:1177	arg1	min					1182:1184	the first 20 min	1169:1184	the first 20 min with saturated steam heat treatment	1169:1220	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	6	17	theme	steam	1112:1116	arg1	treatment					1123:1131	saturated steam heat treatment	1102:1131	saturated steam heat treatment	1102:1131	The shear strength of treated bamboo changed little within 10 min after saturated steam heat treatment, and then decreased rapidly.					
32344634	4	18	from	increase	635:642	arg1	content					668:674	the initial moisture content	647:674	the initial moisture content of bamboo	647:684	With the increase in the initial moisture content of bamboo, the crystallinity of bamboo increased first and then decreased after treatment.					
32344634	7	19	theme	saturated	1191:1199	arg1	treatment					1212:1220	saturated steam heat treatment	1191:1220	saturated steam heat treatment	1191:1220	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	7	20	theme	compressive	1227:1237	arg1	strength					1239:1246	compressive strength	1227:1246	compressive strength	1227:1246	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	6	21	theme	saturated	1102:1110	arg1	treatment					1123:1131	saturated steam heat treatment	1102:1131	saturated steam heat treatment	1102:1131	The shear strength of treated bamboo changed little within 10 min after saturated steam heat treatment, and then decreased rapidly.					
32344634	2	22	theme	steam	366:370	arg1	treatment					377:385	saturated steam heat treatment	356:385	saturated steam heat treatment of bamboo	356:395	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	5	23	theme	bamboo	849:854	arg1	slices					856:861	bamboo slices	849:861	bamboo slices	849:861	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	2	24	theme	saturated	356:364	arg1	treatment					377:385	saturated steam heat treatment	356:385	saturated steam heat treatment of bamboo	356:395	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	1	25	theme	heat	148:151	arg1	time					163:166	heat treatment time	148:166	heat treatment time	148:166	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	7	26	theme	flexural	1272:1279	arg1	modulus					1281:1287	flexural modulus	1272:1287	flexural modulus	1272:1287	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	1	27	theme	treatment	153:161	arg1	time					163:166	heat treatment time	148:166	heat treatment time	148:166	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	0	28	theme	Steam	20:24	arg1	Treatment					31:39	Saturated Steam Heat Treatment	10:39	Saturated Steam Heat Treatment	10:39	Effect of Saturated Steam Heat Treatment on Physical and Chemical Properties of Bamboo.					
32344634	5	29	theme	%	967:967	arg1	content					986:992	a 40% initial moisture content	963:992	a 40% initial moisture content	963:992	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	4	30	theme	bamboo	708:713	arg1	crystallinity					691:703	the crystallinity	687:703	the crystallinity of bamboo	687:713	With the increase in the initial moisture content of bamboo, the crystallinity of bamboo increased first and then decreased after treatment.					
32344634	1	31	theme	time	163:166	arg1	effects					133:139	the effects	129:139	the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties	129:289	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	0	32	theme	Saturated	10:18	arg1	Treatment					31:39	Saturated Steam Heat Treatment	10:39	Saturated Steam Heat Treatment	10:39	Effect of Saturated Steam Heat Treatment on Physical and Chemical Properties of Bamboo.					
32344634	2	33	dep	regulation	468:477	arg1	parameters					547:556	process parameters	539:556	process parameters	539:556	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	4	34	dep	increased	715:723	arg1	decreased					740:748	decreased	740:748	increased first and then decreased after treatment	715:764	With the increase in the initial moisture content of bamboo, the crystallinity of bamboo increased first and then decreased after treatment.					
32344634	5	35	theme	heat	794:797	arg1	treatment					799:807	the saturated steam heat treatment	774:807	the saturated steam heat treatment	774:807	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	0	36	theme	Treatment	31:39	arg1	Effect					0:5	Effect	0:5	Effect of Saturated Steam Heat Treatment on Physical and Chemical Properties of Bamboo.	0:86	Effect of Saturated Steam Heat Treatment on Physical and Chemical Properties of Bamboo.					
32344634	7	37	theme	heat	1207:1210	arg1	treatment					1212:1220	saturated steam heat treatment	1191:1220	saturated steam heat treatment	1191:1220	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	2	38	theme	treatment	377:385	arg1	mechanism					343:351	The mechanism	339:351	The mechanism of saturated steam heat treatment of bamboo	339:395	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	7	39	theme	treated	1310:1316	arg1	bamboo					1318:1323	the treated bamboo	1306:1323	the treated bamboo	1306:1323	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	5	40	theme	cellulose	825:833	arg1	content					814:820	the content	810:820	the content of cellulose and lignin in bamboo slices	810:861	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	1	41	theme	initial	172:178	arg1	content					189:195	initial moisture content	172:195	initial moisture content	172:195	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	0	42	theme	Heat	26:29	arg1	Treatment					31:39	Saturated Steam Heat Treatment	10:39	Saturated Steam Heat Treatment	10:39	Effect of Saturated Steam Heat Treatment on Physical and Chemical Properties of Bamboo.					
32344634	1	43	theme	mechanical	269:278	arg1	properties					280:289	mechanical properties	269:289	mechanical properties	269:289	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	5	44	dep	increased	994:1002	arg1	decreased					1019:1027	decreased	1019:1027	increased first and then decreased	994:1027	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	7	45	theme	flexural	1249:1256	arg1	strength					1258:1265	flexural strength	1249:1265	flexural strength	1249:1265	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	7	46	theme	elasticity	1292:1301	arg1	modulus					1281:1287	flexural modulus	1272:1287	flexural modulus	1272:1287	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	7	46	theme	elasticity	1292:1301	arg1	strength					1239:1246	compressive strength	1227:1246	compressive strength	1227:1246	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	7	46	theme	elasticity	1292:1301	arg1	strength					1258:1265	flexural strength	1249:1265	flexural strength	1249:1265	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	1	47	theme	moisture	180:187	arg1	content					189:195	initial moisture content	172:195	initial moisture content	172:195	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	0	48	theme	Physical	44:51	arg1	Properties					66:75	Physical and Chemical Properties	44:75	Physical and Chemical Properties of Bamboo	44:85	Effect of Saturated Steam Heat Treatment on Physical and Chemical Properties of Bamboo.					
32344634	5	49	theme	saturated	778:786	arg1	treatment					799:807	the saturated steam heat treatment	774:807	the saturated steam heat treatment	774:807	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	5	50	theme	40	965:966	arg1	%					967:967	%	967:967	%	967:967	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	7	51	theme	steam	1201:1205	arg1	treatment					1212:1220	saturated steam heat treatment	1191:1220	saturated steam heat treatment	1191:1220	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	2	52	theme	process	539:545	arg1	parameters					547:556	process parameters	539:556	process parameters	539:556	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	5	53	theme	initial	969:975	arg1	content					986:992	a 40% initial moisture content	963:992	a 40% initial moisture content	963:992	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	3	54	theme	XRD	559:561	arg1	patterns					563:570	XRD patterns	559:570	XRD patterns of the treated bamboo slices	559:599	XRD patterns of the treated bamboo slices were basically the same.					
32344634	3	54	theme	XRD	559:561	arg1	same					620:623	same	620:623	same	620:623	XRD patterns of the treated bamboo slices were basically the same.					
32344634	6	55	theme	bamboo	1060:1065	arg1	strength					1040:1047	The shear strength	1030:1047	The shear strength of treated bamboo	1030:1065	The shear strength of treated bamboo changed little within 10 min after saturated steam heat treatment, and then decreased rapidly.					
32344634	1	56	theme	saturated	297:305	arg1	treatment					318:326	saturated steam heat treatment	297:326	saturated steam heat treatment	297:326	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	2	57	theme	treatment	529:537	arg1	optimization					508:519	the optimization	504:519	the optimization of heat treatment	504:537	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	2	57	theme	treatment	529:537	arg1	regulation					468:477	the regulation	464:477	the regulation of bamboo properties	464:498	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	5	58	theme	moisture	977:984	arg1	content					986:992	a 40% initial moisture content	963:992	a 40% initial moisture content	963:992	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	3	59	theme	bamboo	587:592	arg1	slices					594:599	the treated bamboo slices	575:599	the treated bamboo slices	575:599	XRD patterns of the treated bamboo slices were basically the same.					
32344634	1	60	theme	study	104:108	arg1	aim					92:94	The aim	88:94	The aim of this study	88:108	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	5	61	theme	hemicellulose	894:906	arg1	content					883:889	the content	879:889	the content of hemicellulose	879:906	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	1	62	theme	content	189:195	arg1	effects					133:139	the effects	129:139	the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties	129:289	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	2	63	theme	heat	524:527	arg1	treatment					529:537	heat treatment	524:537	heat treatment	524:537	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	5	64	from	content	814:820	arg1	slices					856:861	bamboo slices	849:861	bamboo slices	849:861	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	3	65	theme	slices	594:599	arg1	patterns					563:570	XRD patterns	559:570	XRD patterns of the treated bamboo slices	559:599	XRD patterns of the treated bamboo slices were basically the same.					
32344634	3	65	theme	slices	594:599	arg1	same					620:623	same	620:623	same	620:623	XRD patterns of the treated bamboo slices were basically the same.					
32344634	4	66	theme	bamboo	679:684	arg1	content					668:674	the initial moisture content	647:674	the initial moisture content of bamboo	647:684	With the increase in the initial moisture content of bamboo, the crystallinity of bamboo increased first and then decreased after treatment.					
32344634	6	67	theme	treated	1052:1058	arg1	bamboo					1060:1065	treated bamboo	1052:1065	treated bamboo	1052:1065	The shear strength of treated bamboo changed little within 10 min after saturated steam heat treatment, and then decreased rapidly.					
32344634	0	68	theme	Chemical	57:64	arg1	Properties					66:75	Physical and Chemical Properties	44:75	Physical and Chemical Properties of Bamboo	44:85	Effect of Saturated Steam Heat Treatment on Physical and Chemical Properties of Bamboo.					
32344634	2	69	theme	heat	372:375	arg1	treatment					377:385	saturated steam heat treatment	356:385	saturated steam heat treatment of bamboo	356:395	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	1	70	dep	time	163:166	arg1	the					144:146	the	144:146	the	144:146	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	2	71	theme	bamboo	390:395	arg1	treatment					377:385	saturated steam heat treatment	356:385	saturated steam heat treatment of bamboo	356:395	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32344634	1	72	theme	bamboo	200:205	arg1	content					189:195	initial moisture content	172:195	initial moisture content	172:195	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	1	72	theme	bamboo	200:205	arg1	time					163:166	heat treatment time	148:166	heat treatment time	148:166	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	5	73	with	bamboo	951:956	arg1	content					986:992	a 40% initial moisture content	963:992	a 40% initial moisture content	963:992	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	1	74	theme	steam	307:311	arg1	treatment					318:326	saturated steam heat treatment	297:326	saturated steam heat treatment	297:326	The aim of this study was to investigate the effects of the heat treatment time and initial moisture content of bamboo on the corresponding chemical composition, crystallinity, and mechanical properties after saturated steam heat treatment at 180 °C.					
32344634	3	75	theme	treated	579:585	arg1	slices					594:599	the treated bamboo slices	575:599	the treated bamboo slices	575:599	XRD patterns of the treated bamboo slices were basically the same.					
32344634	5	76	theme	lignin	839:844	arg1	content					814:820	the content	810:820	the content of cellulose and lignin in bamboo slices	810:861	Due to the saturated steam heat treatment, the content of cellulose and lignin in bamboo slices increased while the content of hemicellulose decreased, but the content of cellulose in bamboo with a 40% initial moisture content increased first and then decreased.					
32344634	7	77	theme	bamboo	1318:1323	arg1	elasticity					1292:1301	elasticity	1292:1301	elasticity of the treated bamboo	1292:1323	During the first 20 min with saturated steam heat treatment, the compressive strength, flexural strength, and flexural modulus of elasticity of the treated bamboo increased, and then decreased.					
32344634	2	78	theme	bamboo	482:487	arg1	properties					489:498	bamboo properties	482:498	bamboo properties	482:498	The mechanism of saturated steam heat treatment of bamboo was revealed on the micro-level, providing a theoretical basis for the regulation of bamboo properties and the optimization of heat treatment process parameters.					
32339582	1	0	theme	microwave	277:285	arg1	treatment					287:295	microwave treatment	277:295	microwave treatment	277:295	The soybean soluble polysaccharide was prepared by mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment.					
32339582	5	1	theme	slight	1352:1357	arg1	changes					1359:1365	slight changes	1352:1365	slight changes in the functional groups	1352:1390	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	4	2	theme	microwave	911:919	arg1	treatment					921:929	microwave treatment	911:929	microwave treatment	911:929	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	3	theme	dialysis	696:703	arg1	capacity					678:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	3	theme	dialysis	696:703	arg1	index					717:721	glucose dialysis retardation index	688:721	glucose dialysis retardation index	688:721	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	3	4	theme	raw	580:582	arg1	materials					584:592	raw materials	580:592	raw materials	580:592	Results revealed that after fermentation treatment, the content of soybean soluble polysaccharide increased to 7.09%, which was 3.16 times that of raw materials, and the microwave treatment was further increased to 7.69%.					
32339582	1	5	theme	mixed	208:212	arg1	fermentation					214:225	mixed fermentation	208:225	mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment	208:295	The soybean soluble polysaccharide was prepared by mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment.					
32339582	4	6	theme	adsorption	667:676	arg1	capacity					678:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	6	theme	adsorption	667:676	arg1	ration					761:766	the α-amylase activity inhibition ration	727:766	the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly	727:825	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	6	theme	adsorption	667:676	arg1	index					717:721	glucose dialysis retardation index	688:721	glucose dialysis retardation index	688:721	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	2	7	theme	structure	328:336	arg1	characteristics					338:352	structure characteristics	328:352	structure characteristics	328:352	The functional properties and structure characteristics of soybean soluble polysaccharide before and after modification were compared.					
32339582	5	8	theme	soybean	1199:1205	arg1	polysaccharide					1215:1228	modified soybean soluble polysaccharide	1190:1228	modified soybean soluble polysaccharide	1190:1228	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	6	9	theme	food	1593:1596	arg1	ingredient					1598:1607	a potential functional food ingredient	1570:1607	a potential functional food ingredient	1570:1607	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	1	10	theme	acid	237:240	arg1	bacteria					242:249	lactic acid bacteria	230:249	lactic acid bacteria	230:249	The soybean soluble polysaccharide was prepared by mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment.					
32339582	5	11	theme	Scanning	1149:1156	arg1	microscopy					1167:1176	Scanning electron microscopy	1149:1176	Scanning electron microscopy	1149:1176	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	12	theme	composition	981:991	arg1	analysis					954:961	the analysis	950:961	the analysis of monosaccharide composition and structural characteristics	950:1022	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	4	13	theme	soybean	771:777	arg1	polysaccharides					787:801	soybean soluble polysaccharides	771:801	soybean soluble polysaccharides increased significantly	771:825	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	5	14	theme	soybean	1417:1423	arg1	polysaccharide					1433:1446	soybean soluble polysaccharide	1417:1446	soybean soluble polysaccharide	1417:1446	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	15	theme	structural	997:1006	arg1	characteristics					1008:1022	structural characteristics	997:1022	structural characteristics	997:1022	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	16	theme	polysaccharide	1433:1446	arg1	structure					1404:1412	crystal structure	1396:1412	crystal structure of soybean soluble polysaccharide	1396:1446	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	16	theme	polysaccharide	1433:1446	arg1	changes					1359:1365	slight changes	1352:1365	slight changes in the functional groups	1352:1390	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	6	17	theme	soybean	1535:1541	arg1	polysaccharide					1551:1564	natural soybean soluble polysaccharide	1527:1564	natural soybean soluble polysaccharide	1527:1564	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	5	18	theme	surface	1243:1249	arg1	area					1251:1254	a larger surface area	1234:1254	a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide	1234:1446	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	0	19	dep	treatment	146:154	arg1	Study					0:4	Study	0:4	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria	0:117	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	4	20	theme	activity	741:748	arg1	capacity					678:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	20	theme	activity	741:748	arg1	ration					761:766	the α-amylase activity inhibition ration	727:766	the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly	727:825	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	0	21	theme	soluble	77:83	arg1	polysaccharides					85:99	soybean soluble polysaccharides	69:99	soybean soluble polysaccharides	69:99	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	0	22	from	Study	0:4	arg1	properties					24:33	functional properties	13:33	functional properties	13:33	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	0	22	from	Study	0:4	arg1	characteristics					50:64	structural characteristics	39:64	structural characteristics	39:64	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	6	23	theme	inexpensive	1505:1515	arg1	okara					1478:1482	okara	1478:1482	okara	1478:1482	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	6	23	theme	inexpensive	1505:1515	arg1	source					1517:1522	a potentially inexpensive source	1491:1522	a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient	1491:1607	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	5	24	contain	has	1230:1232	arg1	polysaccharide					1215:1228	modified soybean soluble polysaccharide	1190:1228	modified soybean soluble polysaccharide	1190:1228	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	24	contain	has	1230:1232	arg2	area					1251:1254	a larger surface area	1234:1254	a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide	1234:1446	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	4	25	theme	flora	848:852	arg1	growth					854:859	intestinal flora growth	837:859	intestinal flora growth	837:859	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	5	26	theme	soybean	1069:1075	arg1	polysaccharide					1085:1098	soybean soluble polysaccharide	1069:1098	soybean soluble polysaccharide	1069:1098	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	6	27	theme	soluble	1543:1549	arg1	polysaccharide					1551:1564	natural soybean soluble polysaccharide	1527:1564	natural soybean soluble polysaccharide	1527:1564	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	4	28	theme	polysaccharides	787:801	arg1	capacity					678:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	28	theme	polysaccharides	787:801	arg1	ration					761:766	the α-amylase activity inhibition ration	727:766	the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly	727:825	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	28	theme	polysaccharides	787:801	arg1	index					717:721	glucose dialysis retardation index	688:721	glucose dialysis retardation index	688:721	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	6	29	theme	ingredient	1598:1607	arg1	okara					1478:1482	okara	1478:1482	okara	1478:1482	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	6	29	theme	ingredient	1598:1607	arg1	source					1517:1522	a potentially inexpensive source	1491:1522	a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient	1491:1607	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	5	30	theme	X-ray	1301:1305	arg1	Diffraction					1307:1317	X-ray Diffraction	1301:1317	X-ray Diffraction	1301:1317	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	31	dep	Fourier	1257:1263	arg1	Transform					1265:1273	Transform	1265:1273	Transform Infrared spectroscopy and X-ray Diffraction	1265:1317	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	6	32	theme	functional	1582:1591	arg1	ingredient					1598:1607	a potential functional food ingredient	1570:1607	a potential functional food ingredient	1570:1607	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	5	33	dep	area	1251:1254	arg1	proved					1319:1324	proved	1319:1324	proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide	1319:1446	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	3	34	theme	soluble	508:514	arg1	polysaccharide					516:529	soybean soluble polysaccharide	500:529	soybean soluble polysaccharide	500:529	Results revealed that after fermentation treatment, the content of soybean soluble polysaccharide increased to 7.09%, which was 3.16 times that of raw materials, and the microwave treatment was further increased to 7.69%.					
32339582	5	35	theme	modification	1125:1136	arg1	treatment					1138:1146	modification treatment	1125:1146	modification treatment	1125:1146	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	4	36	theme	bacteria	898:905	arg1	treatment					921:929	microwave treatment	911:929	microwave treatment	911:929	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	36	theme	bacteria	898:905	arg1	fermentation					876:887	fermentation	876:887	fermentation of mixed bacteria	876:905	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	0	37	theme	structural	39:48	arg1	characteristics					50:64	structural characteristics	39:64	structural characteristics	39:64	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	0	38	dep	properties	24:33	arg1	the					9:11	the	9:11	the	9:11	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	2	39	theme	polysaccharide	373:386	arg1	properties					313:322	The functional properties	298:322	The functional properties	298:322	The functional properties and structure characteristics of soybean soluble polysaccharide before and after modification were compared.					
32339582	2	39	theme	polysaccharide	373:386	arg1	characteristics					338:352	structure characteristics	328:352	structure characteristics	328:352	The functional properties and structure characteristics of soybean soluble polysaccharide before and after modification were compared.					
32339582	1	40	theme	bacteria	242:249	arg1	fermentation					214:225	mixed fermentation	208:225	mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment	208:295	The soybean soluble polysaccharide was prepared by mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment.					
32339582	5	41	theme	polysaccharide	1085:1098	arg1	components					1055:1064	the monosaccharide components	1036:1064	the monosaccharide components of soybean soluble polysaccharide	1036:1098	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	1	42	theme	soybean	161:167	arg1	polysaccharide					177:190	The soybean soluble polysaccharide	157:190	The soybean soluble polysaccharide	157:190	The soybean soluble polysaccharide was prepared by mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment.					
32339582	1	43	theme	Neurospora	255:264	arg1	crassa					266:271	Neurospora crassa	255:271	Neurospora crassa	255:271	The soybean soluble polysaccharide was prepared by mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment.					
32339582	6	44	theme	natural	1527:1533	arg1	polysaccharide					1551:1564	natural soybean soluble polysaccharide	1527:1564	natural soybean soluble polysaccharide	1527:1564	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	3	45	theme	materials	584:592	arg1	times					566:570	3.16 times	561:570	3.16 times that of raw materials	561:592	Results revealed that after fermentation treatment, the content of soybean soluble polysaccharide increased to 7.09%, which was 3.16 times that of raw materials, and the microwave treatment was further increased to 7.69%.					
32339582	2	46	theme	soybean	357:363	arg1	polysaccharide					373:386	soybean soluble polysaccharide	357:386	soybean soluble polysaccharide	357:386	The functional properties and structure characteristics of soybean soluble polysaccharide before and after modification were compared.					
32339582	5	47	from	structure	1404:1412	arg1	groups					1385:1390	the functional groups	1370:1390	the functional groups	1370:1390	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	2	48	theme	functional	302:311	arg1	properties					313:322	The functional properties	298:322	The functional properties	298:322	The functional properties and structure characteristics of soybean soluble polysaccharide before and after modification were compared.					
32339582	6	49	theme	polysaccharide	1551:1564	arg1	okara					1478:1482	okara	1478:1482	okara	1478:1482	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	6	49	theme	polysaccharide	1551:1564	arg1	source					1517:1522	a potentially inexpensive source	1491:1522	a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient	1491:1607	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	5	50	contain	has	1348:1350	arg2	structure					1404:1412	crystal structure	1396:1412	crystal structure of soybean soluble polysaccharide	1396:1446	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	50	contain	has	1348:1350	arg1	modification					1335:1346	the modification	1331:1346	the modification	1331:1346	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	50	contain	has	1348:1350	arg2	changes					1359:1365	slight changes	1352:1365	slight changes in the functional groups	1352:1390	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	4	51	theme	glucose	688:694	arg1	capacity					678:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	51	theme	glucose	688:694	arg1	index					717:721	glucose dialysis retardation index	688:721	glucose dialysis retardation index	688:721	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	3	52	theme	microwave	603:611	arg1	treatment					613:621	the microwave treatment	599:621	the microwave treatment	599:621	Results revealed that after fermentation treatment, the content of soybean soluble polysaccharide increased to 7.09%, which was 3.16 times that of raw materials, and the microwave treatment was further increased to 7.69%.					
32339582	5	53	theme	same	939:942	arg1	time					944:947	the same time	935:947	the same time	935:947	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	4	54	theme	retardation	705:715	arg1	capacity					678:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	54	theme	retardation	705:715	arg1	index					717:721	glucose dialysis retardation index	688:721	glucose dialysis retardation index	688:721	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	0	55	theme	microwave	136:144	arg1	treatment					146:154	fermentation and microwave treatment	119:154	treatment	146:154	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	4	56	theme	glucose	659:665	arg1	capacity					678:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	56	theme	glucose	659:665	arg1	ration					761:766	the α-amylase activity inhibition ration	727:766	the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly	727:825	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	56	theme	glucose	659:665	arg1	index					717:721	glucose dialysis retardation index	688:721	glucose dialysis retardation index	688:721	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	5	57	theme	modified	1190:1197	arg1	polysaccharide					1215:1228	modified soybean soluble polysaccharide	1190:1228	modified soybean soluble polysaccharide	1190:1228	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	1	58	theme	lactic	230:235	arg1	bacteria					242:249	lactic acid bacteria	230:249	lactic acid bacteria	230:249	The soybean soluble polysaccharide was prepared by mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment.					
32339582	5	59	theme	soluble	1207:1213	arg1	polysaccharide					1215:1228	modified soybean soluble polysaccharide	1190:1228	modified soybean soluble polysaccharide	1190:1228	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	60	theme	monosaccharide	966:979	arg1	composition					981:991	monosaccharide composition	966:991	monosaccharide composition	966:991	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	4	61	theme	inhibition	750:759	arg1	capacity					678:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	61	theme	inhibition	750:759	arg1	ration					761:766	the α-amylase activity inhibition ration	727:766	the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly	727:825	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	5	62	from	changes	1359:1365	arg1	groups					1385:1390	the functional groups	1370:1390	the functional groups	1370:1390	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	63	theme	electron	1158:1165	arg1	microscopy					1167:1176	Scanning electron microscopy	1149:1176	Scanning electron microscopy	1149:1176	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	64	theme	soluble	1425:1431	arg1	polysaccharide					1433:1446	soybean soluble polysaccharide	1417:1446	soybean soluble polysaccharide	1417:1446	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	0	65	theme	soybean	69:75	arg1	polysaccharides					85:99	soybean soluble polysaccharides	69:99	soybean soluble polysaccharides	69:99	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	0	66	theme	polysaccharides	85:99	arg1	properties					24:33	functional properties	13:33	functional properties	13:33	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	0	66	theme	polysaccharides	85:99	arg1	characteristics					50:64	structural characteristics	39:64	structural characteristics	39:64	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	1	67	theme	soluble	169:175	arg1	polysaccharide					177:190	The soybean soluble polysaccharide	157:190	The soybean soluble polysaccharide	157:190	The soybean soluble polysaccharide was prepared by mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment.					
32339582	4	68	theme	α-amylase	731:739	arg1	capacity					678:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity	655:685	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	4	68	theme	α-amylase	731:739	arg1	ration					761:766	the α-amylase activity inhibition ration	727:766	the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly	727:825	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	5	69	theme	crystal	1396:1402	arg1	structure					1404:1412	crystal structure	1396:1412	crystal structure of soybean soluble polysaccharide	1396:1446	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	0	70	theme	mixed	104:108	arg1	bacteria					110:117	mixed bacteria	104:117	mixed bacteria	104:117	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	5	71	theme	monosaccharide	1040:1053	arg1	components					1055:1064	the monosaccharide components	1036:1064	the monosaccharide components of soybean soluble polysaccharide	1036:1098	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	0	72	theme	fermentation	119:130	arg1	treatment					146:154	fermentation and microwave treatment	119:154	treatment	146:154	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	4	73	theme	intestinal	837:846	arg1	growth					854:859	intestinal flora growth	837:859	intestinal flora growth	837:859	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	3	74	theme	soybean	500:506	arg1	polysaccharide					516:529	soybean soluble polysaccharide	500:529	soybean soluble polysaccharide	500:529	Results revealed that after fermentation treatment, the content of soybean soluble polysaccharide increased to 7.09%, which was 3.16 times that of raw materials, and the microwave treatment was further increased to 7.69%.					
32339582	5	75	theme	larger	1236:1241	arg1	area					1251:1254	a larger surface area	1234:1254	a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide	1234:1446	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	5	76	theme	characteristics	1008:1022	arg1	analysis					954:961	the analysis	950:961	the analysis of monosaccharide composition and structural characteristics	950:1022	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	4	77	theme	soluble	779:785	arg1	polysaccharides					787:801	soybean soluble polysaccharides	771:801	soybean soluble polysaccharides increased significantly	771:825	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	0	78	theme	functional	13:22	arg1	properties					24:33	functional properties	13:33	functional properties	13:33	Study on the functional properties and structural characteristics of soybean soluble polysaccharides by mixed bacteria fermentation and microwave treatment.					
32339582	3	79	dep	times	566:570	arg1	that					572:575	that	572:575	that	572:575	Results revealed that after fermentation treatment, the content of soybean soluble polysaccharide increased to 7.09%, which was 3.16 times that of raw materials, and the microwave treatment was further increased to 7.69%.					
32339582	6	80	theme	potential	1572:1580	arg1	ingredient					1598:1607	a potential functional food ingredient	1570:1607	a potential functional food ingredient	1570:1607	These results suggested that okara may be a potentially inexpensive source of natural soybean soluble polysaccharide and a potential functional food ingredient.					
32339582	3	81	theme	polysaccharide	516:529	arg1	content					489:495	the content	485:495	the content of soybean soluble polysaccharide	485:529	Results revealed that after fermentation treatment, the content of soybean soluble polysaccharide increased to 7.09%, which was 3.16 times that of raw materials, and the microwave treatment was further increased to 7.69%.					
32339582	5	82	theme	Infrared	1275:1282	arg1	spectroscopy					1284:1295	Infrared spectroscopy	1275:1295	Infrared spectroscopy	1275:1295	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	3	83	theme	fermentation	461:472	arg1	treatment					474:482	fermentation treatment	461:482	fermentation treatment	461:482	Results revealed that after fermentation treatment, the content of soybean soluble polysaccharide increased to 7.09%, which was 3.16 times that of raw materials, and the microwave treatment was further increased to 7.69%.					
32339582	4	84	theme	mixed	892:896	arg1	bacteria					898:905	mixed bacteria	892:905	mixed bacteria	892:905	The glucose adsorption capacity, glucose dialysis retardation index and the α-amylase activity inhibition ration of soybean soluble polysaccharides increased significantly, promotes intestinal flora growth in vitro after fermentation of mixed bacteria and microwave treatment.					
32339582	5	85	theme	soluble	1077:1083	arg1	polysaccharide					1085:1098	soybean soluble polysaccharide	1069:1098	soybean soluble polysaccharide	1069:1098	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
32339582	2	86	theme	soluble	365:371	arg1	polysaccharide					373:386	soybean soluble polysaccharide	357:386	soybean soluble polysaccharide	357:386	The functional properties and structure characteristics of soybean soluble polysaccharide before and after modification were compared.					
32339582	1	87	theme	crassa	266:271	arg1	fermentation					214:225	mixed fermentation	208:225	mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment	208:295	The soybean soluble polysaccharide was prepared by mixed fermentation of lactic acid bacteria and Neurospora crassa and microwave treatment.					
32339582	5	88	theme	functional	1374:1383	arg1	groups					1385:1390	the functional groups	1370:1390	the functional groups	1370:1390	At the same time, the analysis of monosaccharide composition and structural characteristics showed that the monosaccharide components of soybean soluble polysaccharide were redistributed after modification treatment, Scanning electron microscopy showed that modified soybean soluble polysaccharide has a larger surface area; Fourier Transform Infrared spectroscopy and X-ray Diffraction proved that the modification has slight changes in the functional groups and crystal structure of soybean soluble polysaccharide.					
33188978	6	0	theme	high	836:839	arg1	temperatures					853:864	high degradation temperatures	836:864	high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells	836:938	EPS-1 and EPS-2 demonstrated high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells at 2 and 3 mg/mL, respectively.					
33188978	3	1	theme	strain	243:248	arg1	WSUCF1					250:255	strain WSUCF1	243:255	strain WSUCF1	243:255	strain WSUCF1 were investigated.					
33188978	4	2	dep	source	302:307	arg1	mg/L					315:318	525.7 mg/L	309:318	a carbon source 525.7 mg/L of exoproduct	293:332	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	1	3	dep	Characterization	81:96	arg1	WSUCF1					73:78	WSUCF1	73:78	WSUCF1	73:78	WSUCF1: Characterization and bioactivities.					
33188978	5	4	theme	NMR	631:633	arg1	spectra					635:641	their FTIR and NMR spectra	616:641	spectra	635:641	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	6	5	theme	non-cytotoxicity	906:921	arg1	temperatures					853:864	high degradation temperatures	836:864	high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells	836:938	EPS-1 and EPS-2 demonstrated high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells at 2 and 3 mg/mL, respectively.					
33188978	5	6	theme	XRD	720:722	arg1	analysis					724:731	XRD analysis	720:731	XRD analysis	720:731	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	5	7	theme	EPSs	566:569	arg1	kDa					611:613	approximately 1000 kDa	592:613	approximately 1000 kDa	592:613	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	5	7	theme	EPSs	566:569	arg1	weights					550:556	The molecular weights	536:556	The molecular weights of both EPSs	536:569	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	5	8	theme	bonds	687:691	arg1	presence					657:664	the presence	653:664	the presence of α-type glycosidic bonds in a linear structure	653:713	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	7	9	theme	antioxidant	997:1007	arg1	activities					1009:1018	antioxidant activities	997:1018	antioxidant activities	997:1018	In addition, both showed antioxidant activities.					
33188978	5	10	from	presence	657:664	arg1	structure					705:713	a linear structure	696:713	a linear structure	696:713	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	2	11	theme	exopolysaccharides	171:188	arg1	bioactivities					154:166	bioactivities	154:166	bioactivities	154:166	The production, characterization and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp.					
33188978	2	11	theme	exopolysaccharides	171:188	arg1	characterization					133:148	characterization	133:148	characterization	133:148	The production, characterization and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp.					
33188978	2	11	theme	exopolysaccharides	171:188	arg1	production					121:130	production	121:130	production	121:130	The production, characterization and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp.					
33188978	8	12	theme	biomedical	1178:1187	arg1	carriers					1218:1225	drug delivery carriers	1204:1225	drug delivery carriers	1204:1225	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	8	12	theme	biomedical	1178:1187	arg1	fields					1189:1194	biomedical fields	1178:1194	biomedical fields such as drug delivery carriers	1178:1225	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	5	13	theme	low	745:747	arg1	degree					749:754	a low degree	743:754	a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2)	743:804	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	2	14	from	sp	239:240	arg1	bioactivities					154:166	bioactivities	154:166	bioactivities	154:166	The production, characterization and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp.					
33188978	2	14	from	sp	239:240	arg1	characterization					133:148	characterization	133:148	characterization	133:148	The production, characterization and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp.					
33188978	2	14	from	sp	239:240	arg1	production					121:130	production	121:130	production	121:130	The production, characterization and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp.					
33188978	5	15	theme	linear	698:703	arg1	structure					705:713	a linear structure	696:713	a linear structure	696:713	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	5	16	theme	molecular	540:548	arg1	kDa					611:613	approximately 1000 kDa	592:613	approximately 1000 kDa	592:613	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	5	16	theme	molecular	540:548	arg1	weights					550:556	The molecular weights	536:556	The molecular weights of both EPSs	536:569	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	5	17	theme	0.27	793:796	arg1	crystallinity					759:771	crystallinity	759:771	crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2)	759:804	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	0	18	theme	new	4:6	arg1	exopolysaccharides					8:25	Two new exopolysaccharides	0:25	Two new exopolysaccharides from a thermophilic bacterium Geobacillus sp	0:70	Two new exopolysaccharides from a thermophilic bacterium Geobacillus sp.					
33188978	4	19	theme	molar	472:476	arg1	ratio					478:482	a molar ratio	470:482	a molar ratio of 1:0.21	470:492	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	8	20	theme	microorganisms	1076:1089	arg1	applications					1060:1071	the applications	1056:1071	the applications of microorganisms isolated from extreme environments	1056:1124	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	4	21	theme	purified	383:390	arg1	EPSs					392:395	Two purified EPSs	379:395	Two purified EPSs	379:395	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	5	22	theme	crystallinity	759:771	arg1	degree					749:754	a low degree	743:754	a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2)	743:804	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	6	23	theme	°C	884:885	arg1	temperatures					853:864	high degradation temperatures	836:864	high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells	836:938	EPS-1 and EPS-2 demonstrated high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells at 2 and 3 mg/mL, respectively.					
33188978	8	24	theme	extreme	1105:1111	arg1	environments					1113:1124	extreme environments	1105:1124	extreme environments	1105:1124	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	5	25	theme	0.11	776:779	arg1	crystallinity					759:771	crystallinity	759:771	crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2)	759:804	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	4	26	theme	carbon	295:300	arg1	glucose					282:288	glucose	282:288	glucose	282:288	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	4	26	theme	carbon	295:300	arg1	source					302:307	a carbon source 525.7 mg/L	293:318	a carbon source 525.7 mg/L of exoproduct	293:332	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	0	27	theme	thermophilic	34:45	arg1	sp					69:70	a thermophilic bacterium Geobacillus sp	32:70	a thermophilic bacterium Geobacillus sp	32:70	Two new exopolysaccharides from a thermophilic bacterium Geobacillus sp.					
33188978	5	28	attach	presence	657:664	arg2	bonds					687:691	α-type glycosidic bonds	669:691	α-type glycosidic bonds	669:691	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	5	28	attach	presence	657:664	arg1	structure					705:713	a linear structure	696:713	a linear structure	696:713	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	5	29	theme	α-type	669:674	arg1	bonds					687:691	α-type glycosidic bonds	669:691	α-type glycosidic bonds	669:691	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	8	30	from	WSUCF1	1038:1043	arg1	EPSs					1021:1024	EPSs	1021:1024	EPSs from strain WSUCF1	1021:1043	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	4	31	contain	containing	436:445	arg2	glucose					459:465	glucose	459:465	glucose	459:465	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	4	31	contain	containing	436:445	arg2	mannose					447:453	mannose	447:453	mannose	447:453	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	4	31	contain	containing	436:445	arg1	glucomannan					424:434	a glucomannan	422:434	a glucomannan containing mannose and glucose	422:465	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	4	31	contain	containing	436:445	arg1	EPS-1					412:416	EPS-1	412:416	EPS-1	412:416	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	2	32	dep	production	121:130	arg1	The					117:119	The	117:119	The	117:119	The production, characterization and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp.					
33188978	5	33	theme	glycosidic	676:685	arg1	bonds					687:691	α-type glycosidic bonds	669:691	α-type glycosidic bonds	669:691	The molecular weights of both EPSs were estimated to be approximately 1000 kDa, their FTIR and NMR spectra indicated the presence of α-type glycosidic bonds in a linear structure, and XRD analysis indicated a low degree of crystallinity of 0.11 (EPS-1) and 0.27 (EPS-2).					
33188978	8	34	attach	isolated	1091:1098	arg2	microorganisms					1076:1089	microorganisms	1076:1089	microorganisms isolated from extreme environments	1076:1124	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	8	34	attach	isolated	1091:1098	arg1	environments					1113:1124	extreme environments	1105:1124	extreme environments	1105:1124	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	4	35	theme	40-L	353:356	arg1	bioreactor					358:367	a 40-L bioreactor	351:367	a 40-L bioreactor	351:367	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	0	36	theme	Geobacillus	57:67	arg1	sp					69:70	a thermophilic bacterium Geobacillus sp	32:70	a thermophilic bacterium Geobacillus sp	32:70	Two new exopolysaccharides from a thermophilic bacterium Geobacillus sp.					
33188978	6	37	theme	°C	873:874	arg1	temperatures					853:864	high degradation temperatures	836:864	high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells	836:938	EPS-1 and EPS-2 demonstrated high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells at 2 and 3 mg/mL, respectively.					
33188978	4	38	from	glucomannan	424:434	arg1	ratio					478:482	a molar ratio	470:482	a molar ratio of 1:0.21	470:492	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	0	39	from	sp	69:70	arg1	exopolysaccharides					8:25	Two new exopolysaccharides	0:25	Two new exopolysaccharides from a thermophilic bacterium Geobacillus sp	0:70	Two new exopolysaccharides from a thermophilic bacterium Geobacillus sp.					
33188978	8	40	theme	drug	1204:1207	arg1	carriers					1218:1225	drug delivery carriers	1204:1225	drug delivery carriers	1204:1225	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	0	41	theme	bacterium	47:55	arg1	sp					69:70	a thermophilic bacterium Geobacillus sp	32:70	a thermophilic bacterium Geobacillus sp	32:70	Two new exopolysaccharides from a thermophilic bacterium Geobacillus sp.					
33188978	6	42	theme	degradation	841:851	arg1	temperatures					853:864	high degradation temperatures	836:864	high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells	836:938	EPS-1 and EPS-2 demonstrated high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells at 2 and 3 mg/mL, respectively.					
33188978	2	43	theme	Geobacillus	227:237	arg1	sp					239:240	a thermophilic bacterium Geobacillus sp	202:240	a thermophilic bacterium Geobacillus sp	202:240	The production, characterization and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp.					
33188978	2	44	theme	bacterium	217:225	arg1	sp					239:240	a thermophilic bacterium Geobacillus sp	202:240	a thermophilic bacterium Geobacillus sp	202:240	The production, characterization and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp.					
33188978	2	45	theme	thermophilic	204:215	arg1	sp					239:240	a thermophilic bacterium Geobacillus sp	202:240	a thermophilic bacterium Geobacillus sp	202:240	The production, characterization and bioactivities of exopolysaccharides (EPSs) from a thermophilic bacterium Geobacillus sp.					
33188978	8	46	from	exploitation	1162:1173	arg1	carriers					1218:1225	drug delivery carriers	1204:1225	drug delivery carriers	1204:1225	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	8	46	from	exploitation	1162:1173	arg1	fields					1189:1194	biomedical fields	1178:1194	biomedical fields such as drug delivery carriers	1178:1225	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	8	47	theme	delivery	1209:1216	arg1	carriers					1218:1225	drug delivery carriers	1204:1225	drug delivery carriers	1204:1225	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	4	48	dep	°C.	375:377	arg1	obtained					402:409	obtained	402:409	were obtained	397:409	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	8	49	theme	strain	1031:1036	arg1	WSUCF1					1038:1043	strain WSUCF1	1031:1043	strain WSUCF1	1031:1043	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	4	50	theme	exoproduct	323:332	arg1	glucose					282:288	glucose	282:288	glucose	282:288	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	4	50	theme	exoproduct	323:332	arg1	source					302:307	a carbon source 525.7 mg/L	293:318	a carbon source 525.7 mg/L of exoproduct	293:332	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
33188978	8	51	theme	valuable	1140:1147	arg1	resource					1149:1156	a valuable resource	1138:1156	a valuable resource for exploitation in biomedical fields such as drug delivery carriers	1138:1225	EPSs from strain WSUCF1 may expand the applications of microorganisms isolated from extreme environments and provide a valuable resource for exploitation in biomedical fields such as drug delivery carriers.					
33188978	6	52	theme	HEK-293	926:932	arg1	cells					934:938	HEK-293 cells	926:938	HEK-293 cells	926:938	EPS-1 and EPS-2 demonstrated high degradation temperatures of 319 °C and 314 °C, respectively, and non-cytotoxicity to HEK-293 cells at 2 and 3 mg/mL, respectively.					
33188978	4	53	theme	1:0.21	487:492	arg1	ratio					478:482	a molar ratio	470:482	a molar ratio of 1:0.21	470:492	Using glucose as a carbon source 525.7 mg/L of exoproduct were produced in a 40-L bioreactor at 60 °C. Two purified EPSs were obtained: EPS-1 was a glucomannan containing mannose and glucose in a molar ratio of 1:0.21, while EPS-2 was composed of mannan only.					
31883116	6	0	theme	new	1368:1370	arg1	hypotheses					1372:1381	new hypotheses	1368:1381	new hypotheses concerning the formation of these complex lipopolysaccharide edifices	1368:1451	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	2	1	with	association	367:377	arg1	polyester					390:398	cutin polyester	384:398	cutin polyester	384:398	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	4	2	theme	specific	857:864	arg1	degree					903:908	a high degree	896:908	a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI),	896:1010	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	4	2	theme	specific	857:864	arg1	crystallinity					1023:1035	a high crystallinity	1016:1035	a high crystallinity of cellulose	1016:1048	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	4	2	theme	specific	857:864	arg1	features					877:884	specific structural features	857:884	specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose	857:1048	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	5	3	theme	specific	1139:1146	arg1	modification					1148:1159	a specific modification	1137:1159	a specific modification of CEPs	1137:1167	In addition to decreasing cutin deposition and polymerization, cus1 silencing induced a specific modification of CEPs, especially on pectin content, while NCPs were not affected.					
31883116	3	4	from	impact	756:761	arg1	structure					799:807	the fine structure	790:807	the fine structure of CEPs	790:815	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	0	5	theme	tomato	12:17	arg1	cuticles					25:32	tomato fruit cuticles	12:32	tomato fruit cuticles	12:32	Assembly of tomato fruit cuticles: a cross-talk between the cutin polyester and cell wall polysaccharides.					
31883116	0	6	theme	cutin	60:64	arg1	polyester					66:74	the cutin polyester	56:74	the cutin polyester	56:74	Assembly of tomato fruit cuticles: a cross-talk between the cutin polyester and cell wall polysaccharides.					
31883116	3	7	theme	tomato	667:672	arg1	fruits					674:679	tomato fruits	667:679	tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1)	667:741	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	1	8	theme	ubiquitous	139:148	arg1	composite					169:177	an essential and ubiquitous biological polymer composite	122:177	an essential and ubiquitous biological polymer composite	122:177	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	1	8	theme	ubiquitous	139:148	arg1	cuticle					111:117	The cuticle	107:117	The cuticle	107:117	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	4	9	theme	cellulose	1040:1048	arg1	degree					903:908	a high degree	896:908	a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI),	896:1010	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	4	9	theme	cellulose	1040:1048	arg1	crystallinity					1023:1035	a high crystallinity	1016:1035	a high crystallinity of cellulose	1016:1048	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	1	10	theme	cutin	243:247	arg1	component					226:234	component	226:234	component	226:234	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	1	10	theme	cutin	243:247	arg1	polyester					249:257	the cutin polyester	239:257	the cutin polyester entangled with cell wall polysaccharides	239:298	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	0	11	theme	cell	80:83	arg1	polysaccharides					90:104	cell wall polysaccharides	80:104	cell wall polysaccharides	80:104	Assembly of tomato fruit cuticles: a cross-talk between the cutin polyester and cell wall polysaccharides.					
31883116	6	12	theme	complex	1417:1423	arg1	edifices					1444:1451	these complex lipopolysaccharide edifices	1411:1451	these complex lipopolysaccharide edifices	1411:1451	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	4	13	theme	low	966:968	arg1	esterification					913:926	esterification	913:926	esterification (i.e. methylation and acetylation)	913:961	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	4	13	theme	low	966:968	arg1	ramification					970:981	a low ramification	964:981	a low ramification of rhamnogalacturonan (RGI)	964:1009	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	2	14	theme	biophysical	519:529	arg1	methods					531:537	biochemical and biophysical methods	503:537	biochemical and biophysical methods	503:537	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	2	15	theme	CEPs	587:590	arg1	structure					574:582	the fine structure	565:582	the fine structure of CEPs	565:590	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	2	16	with	nature	305:310	arg1	polyester					390:398	cutin polyester	384:398	cutin polyester	384:398	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	5	17	theme	cutin	1077:1081	arg1	deposition					1083:1092	cutin deposition	1077:1092	cutin deposition	1077:1092	In addition to decreasing cutin deposition and polymerization, cus1 silencing induced a specific modification of CEPs, especially on pectin content, while NCPs were not affected.					
31883116	6	18	theme	new	1235:1237	arg1	evidence					1239:1246	This new evidence	1230:1246	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides	1230:1357	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	4	19	theme	high	1018:1021	arg1	crystallinity					1023:1035	a high crystallinity	1016:1035	a high crystallinity of cellulose	1016:1048	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	3	20	theme	CEPs	812:815	arg1	structure					799:807	the fine structure	790:807	the fine structure of CEPs	790:815	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	1	21	theme	biological	150:159	arg1	composite					169:177	an essential and ubiquitous biological polymer composite	122:177	an essential and ubiquitous biological polymer composite	122:177	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	1	21	theme	biological	150:159	arg1	cuticle					111:117	The cuticle	107:117	The cuticle	107:117	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	0	22	theme	wall	85:88	arg1	polysaccharides					90:104	cell wall polysaccharides	80:104	cell wall polysaccharides	80:104	Assembly of tomato fruit cuticles: a cross-talk between the cutin polyester and cell wall polysaccharides.					
31883116	3	23	theme	transgenic	702:711	arg1	lines					713:717	cutin-deficient transgenic lines cus1 (cutin synthase 1)	686:741	cutin-deficient transgenic lines cus1 (cutin synthase 1)	686:741	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	6	24	theme	cutin	1318:1322	arg1	polymerization					1324:1337	cutin polymerization	1318:1337	cutin polymerization	1318:1337	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	5	25	dep	decreasing	1066:1075	arg1	addition					1054:1061	addition	1054:1061	addition	1054:1061	In addition to decreasing cutin deposition and polymerization, cus1 silencing induced a specific modification of CEPs, especially on pectin content, while NCPs were not affected.					
31883116	1	26	theme	polymer	161:167	arg1	composite					169:177	an essential and ubiquitous biological polymer composite	122:177	an essential and ubiquitous biological polymer composite	122:177	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	1	26	theme	polymer	161:167	arg1	cuticle					111:117	The cuticle	107:117	The cuticle	107:117	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	2	27	theme	enzymatic	465:473	arg1	pretreatments					475:487	chemical and enzymatic pretreatments	452:487	chemical and enzymatic pretreatments combined with biochemical and biophysical methods	452:537	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	2	28	theme	noncutinized	609:620	arg1	NCPs					639:642	NCPs	639:642	NCPs	639:642	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	2	28	theme	noncutinized	609:620	arg1	polysaccharides					622:636	the noncutinized polysaccharides	605:636	the noncutinized polysaccharides (NCPs)	605:643	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	6	29	theme	specificities	1266:1278	arg1	evidence					1239:1246	This new evidence	1230:1246	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides	1230:1357	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	3	30	from	lines	713:717	arg1	fruits					674:679	tomato fruits	667:679	tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1)	667:741	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	4	31	theme	Cutin-embedded	818:831	arg1	polysaccharides					833:847	Cutin-embedded polysaccharides	818:847	Cutin-embedded polysaccharides	818:847	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	4	32	theme	structural	866:875	arg1	degree					903:908	a high degree	896:908	a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI),	896:1010	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	4	32	theme	structural	866:875	arg1	crystallinity					1023:1035	a high crystallinity	1016:1035	a high crystallinity of cellulose	1016:1048	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	4	32	theme	structural	866:875	arg1	features					877:884	specific structural features	857:884	specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose	857:1048	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	0	33	theme	fruit	19:23	arg1	cuticles					25:32	tomato fruit cuticles	12:32	tomato fruit cuticles	12:32	Assembly of tomato fruit cuticles: a cross-talk between the cutin polyester and cell wall polysaccharides.					
31883116	1	34	theme	cell	274:277	arg1	polysaccharides					284:298	cell wall polysaccharides	274:298	cell wall polysaccharides	274:298	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	4	35	theme	esterification	913:926	arg1	degree					903:908	a high degree	896:908	a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI),	896:1010	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	4	35	theme	esterification	913:926	arg1	crystallinity					1023:1035	a high crystallinity	1016:1035	a high crystallinity of cellulose	1016:1048	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	3	36	theme	fine	794:797	arg1	structure					799:807	the fine structure	790:807	the fine structure of CEPs	790:815	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	1	37	theme	aerial	188:193	arg1	organs					201:206	aerial plant organs	188:206	aerial plant organs	188:206	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	2	38	theme	tomato	427:432	arg1	fruit					434:438	tomato fruit	427:438	tomato fruit	427:438	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	2	38	theme	tomato	427:432	arg1	model					445:449	a model	443:449	a model	443:449	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	1	39	theme	wall	279:282	arg1	polysaccharides					284:298	cell wall polysaccharides	274:298	cell wall polysaccharides	274:298	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	6	40	theme	cross-talk	1299:1308	arg1	specificities					1266:1278	the structural specificities	1251:1278	the structural specificities of CEPs and of the cross-talk between cutin polymerization	1251:1337	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	6	40	theme	cross-talk	1299:1308	arg1	polysaccharides					1343:1357	polysaccharides	1343:1357	polysaccharides	1343:1357	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	1	41	theme	plant	195:199	arg1	organs					201:206	aerial plant organs	188:206	aerial plant organs	188:206	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	4	42	theme	rhamnogalacturonan	986:1003	arg1	esterification					913:926	esterification	913:926	esterification (i.e. methylation and acetylation)	913:961	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	4	42	theme	rhamnogalacturonan	986:1003	arg1	ramification					970:981	a low ramification	964:981	a low ramification of rhamnogalacturonan (RGI)	964:1009	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	6	43	theme	structural	1255:1264	arg1	specificities					1266:1278	the structural specificities	1251:1278	the structural specificities of CEPs and of the cross-talk between cutin polymerization	1251:1337	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	5	44	theme	pectin	1184:1189	arg1	content					1191:1197	pectin content	1184:1197	pectin content	1184:1197	In addition to decreasing cutin deposition and polymerization, cus1 silencing induced a specific modification of CEPs, especially on pectin content, while NCPs were not affected.					
31883116	2	45	theme	chemical	452:459	arg1	pretreatments					475:487	chemical and enzymatic pretreatments	452:487	chemical and enzymatic pretreatments combined with biochemical and biophysical methods	452:537	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	0	46	theme	cuticles	25:32	arg1	Assembly					0:7	Assembly	0:7	Assembly of tomato fruit cuticles: a cross-talk between the cutin polyester and cell wall polysaccharides.	0:105	Assembly of tomato fruit cuticles: a cross-talk between the cutin polyester and cell wall polysaccharides.					
31883116	6	47	theme	lipopolysaccharide	1425:1442	arg1	edifices					1444:1451	these complex lipopolysaccharide edifices	1411:1451	these complex lipopolysaccharide edifices	1411:1451	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	2	48	theme	biochemical	503:513	arg1	methods					531:537	biochemical and biophysical methods	503:537	biochemical and biophysical methods	503:537	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	3	49	dep	lines	713:717	arg1	cus1					719:722	cus1	719:722	cutin-deficient transgenic lines cus1 (cutin synthase 1)	686:741	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	3	49	dep	lines	713:717	arg1	synthase					731:738	cutin synthase 1	725:740	cutin synthase 1	725:740	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	6	50	theme	polysaccharides	1343:1357	arg1	evidence					1239:1246	This new evidence	1230:1246	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides	1230:1357	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	5	51	theme	CEPs	1164:1167	arg1	modification					1148:1159	a specific modification	1137:1159	a specific modification of CEPs	1137:1167	In addition to decreasing cutin deposition and polymerization, cus1 silencing induced a specific modification of CEPs, especially on pectin content, while NCPs were not affected.					
31883116	6	52	theme	edifices	1444:1451	arg1	formation					1398:1406	the formation	1394:1406	the formation of these complex lipopolysaccharide edifices	1394:1451	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	5	53	theme	cus1	1114:1117	arg1	silencing					1119:1127	cus1 silencing	1114:1127	cus1 silencing	1114:1127	In addition to decreasing cutin deposition and polymerization, cus1 silencing induced a specific modification of CEPs, especially on pectin content, while NCPs were not affected.					
31883116	4	54	dep	methylation	934:944	arg1	i.e.					929:932	i.e.	929:932	i.e.	929:932	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	3	55	used	used	662:665	arg2	we					659:660	we	659:660	we	659:660	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	6	56	theme	CEPs	1283:1286	arg1	specificities					1266:1278	the structural specificities	1251:1278	the structural specificities of CEPs and of the cross-talk between cutin polymerization	1251:1337	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	6	56	theme	CEPs	1283:1286	arg1	polysaccharides					1343:1357	polysaccharides	1343:1357	polysaccharides	1343:1357	This new evidence of the structural specificities of CEPs and of the cross-talk between cutin polymerization and polysaccharides provides new hypotheses concerning the formation of these complex lipopolysaccharide edifices.					
31883116	2	57	theme	polysaccharides	334:348	arg1	unresolved					410:419	unresolved	410:419	unresolved	410:419	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	2	57	theme	polysaccharides	334:348	arg1	association					367:377	their association	361:377	their association with cutin polyester	361:398	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	2	57	theme	polysaccharides	334:348	arg1	nature					305:310	The nature	301:310	The nature of the cutin-embedded polysaccharides (CEPs)	301:355	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	2	58	theme	fine	569:572	arg1	structure					574:582	the fine structure	565:582	the fine structure of CEPs	565:590	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	2	59	theme	cutin-embedded	319:332	arg1	polysaccharides					334:348	the cutin-embedded polysaccharides	315:348	the cutin-embedded polysaccharides (CEPs)	315:355	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	2	59	theme	cutin-embedded	319:332	arg1	CEPs					351:354	CEPs	351:354	CEPs	351:354	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	3	60	theme	polymerization	772:785	arg1	impact					756:761	the impact	752:761	the impact of cutin polymerization on the fine structure of CEPs	752:815	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	4	61	theme	high	898:901	arg1	degree					903:908	a high degree	896:908	a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI),	896:1010	Cutin-embedded polysaccharides exhibit specific structural features including a high degree of esterification (i.e. methylation and acetylation), a low ramification of rhamnogalacturonan (RGI), and a high crystallinity of cellulose.					
31883116	3	62	theme	cutin	725:729	arg1	cus1					719:722	cus1	719:722	cutin-deficient transgenic lines cus1 (cutin synthase 1)	686:741	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	3	62	theme	cutin	725:729	arg1	synthase					731:738	cutin synthase 1	725:740	cutin synthase 1	725:740	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	1	63	theme	structural	215:224	arg1	component					226:234	component	226:234	component	226:234	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	1	63	theme	structural	215:224	arg1	polyester					249:257	the cutin polyester	239:257	the cutin polyester entangled with cell wall polysaccharides	239:298	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	2	64	theme	cutin	384:388	arg1	polyester					390:398	cutin polyester	384:398	cutin polyester	384:398	The nature of the cutin-embedded polysaccharides (CEPs) and their association with cutin polyester are still unresolved Using tomato fruit as a model, chemical and enzymatic pretreatments combined with biochemical and biophysical methods were developed to compare the fine structure of CEPs with that of the noncutinized polysaccharides (NCPs).					
31883116	0	65	dep	Assembly	0:7	arg1	cross-talk					37:46	a cross-talk	35:46	Assembly of tomato fruit cuticles: a cross-talk between the cutin polyester and cell wall polysaccharides.	0:105	Assembly of tomato fruit cuticles: a cross-talk between the cutin polyester and cell wall polysaccharides.					
31883116	3	66	theme	cutin-deficient	686:700	arg1	lines					713:717	cutin-deficient transgenic lines cus1 (cutin synthase 1)	686:741	cutin-deficient transgenic lines cus1 (cutin synthase 1)	686:741	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
31883116	1	67	theme	essential	125:133	arg1	composite					169:177	an essential and ubiquitous biological polymer composite	122:177	an essential and ubiquitous biological polymer composite	122:177	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	1	67	theme	essential	125:133	arg1	cuticle					111:117	The cuticle	107:117	The cuticle	107:117	The cuticle is an essential and ubiquitous biological polymer composite covering aerial plant organs, whose structural component is the cutin polyester entangled with cell wall polysaccharides.					
31883116	3	68	theme	cutin	766:770	arg1	polymerization					772:785	cutin polymerization	766:785	cutin polymerization	766:785	In addition, we used tomato fruits from cutin-deficient transgenic lines cus1 (cutin synthase 1) to study the impact of cutin polymerization on the fine structure of CEPs.					
34298931	7	0	theme	young	1040:1044	arg1	tissues					1046:1052	the young tissues	1036:1052	the young tissues from pathogens	1036:1067	The dense glandular trichomes provide mechanical protection for young tissues; additionally, their secretion protects the young tissues from pathogens by a special chemical component.					
34298931	5	1	from	trichomes	651:659	arg1	trees					677:681	R. potaninii trees	664:681	R. potaninii trees	664:681	The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8-15 cells.					
34298931	6	2	theme	glandular	805:813	arg1	trichomes					815:823	glandular trichomes	805:823	glandular trichomes	805:823	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	5	3	with	structure	717:725	arg1	cells					748:752	8-15 cells	743:752	8-15 cells	743:752	The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8-15 cells.					
34298931	4	4	theme	different	595:603	arg1	regions					605:611	different regions	595:611	different regions	595:611	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	10	5	theme	most	1460:1463	arg1	subpathways					1465:1475	most subpathways	1460:1475	most subpathways	1460:1475	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	11	6	theme	chitinase	1566:1574	arg1	activity					1576:1583	the chitinase activity	1562:1583	the chitinase activity	1562:1583	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	10	7	theme	genes	1341:1345	arg1	numbers					1305:1311	The numbers	1301:1311	The numbers of differentially expressed genes	1301:1345	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	10	7	theme	genes	1341:1345	arg1	large					1352:1356	large	1352:1356	large	1352:1356	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	6	8	contain	has	825:827	arg1	secretion					792:800	the secretion	788:800	the secretion of glandular trichomes	788:823	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	6	8	contain	has	825:827	arg2	lipids					882:887	acidic lipids	875:887	acidic lipids	875:887	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	6	8	contain	has	825:827	arg2	compounds					860:868	phenolic compounds	851:868	phenolic compounds	851:868	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	6	8	contain	has	825:827	arg2	lipids					910:915	few neutral lipids	898:915	many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids	829:915	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	6	8	contain	has	825:827	arg2	polysaccharides					834:848	many polysaccharides	829:848	many polysaccharides	829:848	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	2	9	theme	high	271:274	arg1	abundance					276:284	a high abundance	269:284	a high abundance of tannins	269:295	It produces galls (induced by aphids) with a high abundance of tannins.					
34298931	4	10	from	leaf	587:590	arg1	stages					617:622	stages	617:622	stages	617:622	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	4	10	from	leaf	587:590	arg1	regions					605:611	different regions	595:611	different regions	595:611	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	8	11	theme	transcriptome	1115:1127	arg1	analysis					1129:1136	transcriptome analysis	1115:1136	transcriptome analysis	1115:1136	According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids.					
34298931	6	12	theme	acidic	875:880	arg1	lipids					882:887	acidic lipids	875:887	acidic lipids	875:887	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	0	13	from	Pattern	67:73	arg1	the					101:103	the	101:103	the	101:103	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	4	14	theme	leaf	587:590	arg1	sides					576:580	the adaxial and abaxial sides	552:580	the adaxial and abaxial sides of a leaf in different regions and stages of the leaf	552:634	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	1	15	theme	tree	203:206	arg1	species					208:214	an economically and medicinally important tree species	161:214	an economically and medicinally important tree species in China	161:223	Rhus potaninii Maxim is an economically and medicinally important tree species in China.					
34298931	1	15	theme	tree	203:206	arg1	Maxim					152:156	Maxim	152:156	Maxim	152:156	Rhus potaninii Maxim is an economically and medicinally important tree species in China.					
34298931	6	16	theme	phenolic	851:858	arg1	compounds					860:868	phenolic compounds	851:868	phenolic compounds	851:868	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	1	17	from	species	208:214	arg1	China					219:223	China	219:223	China	219:223	Rhus potaninii Maxim is an economically and medicinally important tree species in China.					
34298931	11	18	theme	responsible	1546:1556	arg1	trichomes					1535:1543	glandular trichomes	1525:1543	glandular trichomes	1525:1543	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	7	19	theme	mechanical	956:965	arg1	protection					967:976	mechanical protection	956:976	mechanical protection for young tissues	956:994	The dense glandular trichomes provide mechanical protection for young tissues; additionally, their secretion protects the young tissues from pathogens by a special chemical component.					
34298931	2	20	with	galls	238:242	arg1	abundance					276:284	a high abundance	269:284	a high abundance of tannins	269:295	It produces galls (induced by aphids) with a high abundance of tannins.					
34298931	5	21	with	stalk	694:698	arg1	structure					717:725	cellular structure	708:725	cellular structure	708:725	The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8-15 cells.					
34298931	5	21	with	stalk	694:698	arg1	head					733:736	a head	731:736	a head	731:736	The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8-15 cells.					
34298931	8	22	theme	amino	1219:1223	arg1	acids					1225:1229	toxic amino acids	1213:1229	toxic amino acids	1213:1229	According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids.					
34298931	11	23	theme	plant	1672:1676	arg1	defense					1678:1684	plant defense	1672:1684	plant defense	1672:1684	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	0	24	theme	Gene	51:54	arg1	Pattern					67:73	Gene Expression Pattern	51:73	Gene Expression Pattern	51:73	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	5	25	theme	cellular	708:715	arg1	structure					717:725	cellular structure	708:725	cellular structure	708:725	The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8-15 cells.					
34298931	0	26	from	Distribution	11:22	arg1	the					101:103	the	101:103	the	101:103	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	4	27	theme	leaf	631:634	arg1	stages					617:622	stages	617:622	stages	617:622	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	4	27	theme	leaf	631:634	arg1	regions					605:611	different regions	595:611	different regions	595:611	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	12	28	theme	R.	1817:1818	arg1	surface					1806:1812	the leaf surface	1797:1812	the leaf surface of R. potaninii	1797:1828	To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.					
34298931	6	29	theme	few	898:900	arg1	lipids					910:915	few neutral lipids	898:915	many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids	829:915	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	0	30	from	Structure	0:8	arg1	the					101:103	the	101:103	the	101:103	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	0	31	theme	Glandular	78:86	arg1	Trichomes					88:96	Glandular Trichomes	78:96	Glandular Trichomes	78:96	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	3	32	from	histology	319:327	arg1	the					444:446	the	444:446	the	444:446	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	33	dep	the	444:446	arg1	leaves					448:453	leaves	448:453	leaves	448:453	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	34	theme	macromolecular	382:395	arg1	components					397:406	the macromolecular components	378:406	the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii	378:469	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	12	35	dep	R.	1817:1818	arg1	potaninii					1820:1828	R. potaninii	1817:1828	R. potaninii	1817:1828	To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.					
34298931	3	36	theme	cellular	330:337	arg1	histology					319:327	histology	319:327	histology	319:327	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	36	theme	cellular	330:337	arg1	structures					339:348	cellular structures	330:348	cellular structures	330:348	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	11	37	theme	expressed	1495:1503	arg1	genes					1505:1509	Some selectively expressed genes	1478:1509	Some selectively expressed genes	1478:1509	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	8	38	dep	found	1142:1146	arg1	enhanced					1148:1155	enhanced	1148:1155	found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids	1142:1251	According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids.					
34298931	3	39	theme	glandular	421:429	arg1	trichomes					431:439	secretive glandular trichomes	411:439	secretive glandular trichomes on the leaves of R. potaninii	411:469	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	5	40	theme	8-15	743:746	arg1	cells					748:752	8-15 cells	743:752	8-15 cells	743:752	The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8-15 cells.					
34298931	4	41	theme	trichomes	512:520	arg1	density					491:497	the density	487:497	the density of glandular trichomes and tomenta	487:532	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	10	42	theme	upregulated	1390:1400	arg1	genes					1402:1406	upregulated genes	1390:1406	upregulated genes	1390:1406	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	8	43	theme	biosynthetic	1157:1168	arg1	pathways					1185:1192	biosynthetic and metabolism pathways	1157:1192	pathways	1185:1192	According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids.					
34298931	9	44	theme	similar	1267:1273	arg1	tendency					1275:1282	a similar tendency	1265:1282	a similar tendency to the staining	1265:1298	This shows a similar tendency to the staining.					
34298931	0	45	theme	Chemical	25:32	arg1	Composition					34:44	Chemical Composition	25:44	Chemical Composition	25:44	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	4	46	from	variation	474:482	arg1	density					491:497	the density	487:497	the density of glandular trichomes and tomenta	487:532	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	12	47	from	components	1741:1750	arg1	trichomes					1784:1792	glandular trichomes	1774:1792	glandular trichomes	1774:1792	To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.					
34298931	8	48	theme	metabolism	1174:1183	arg1	pathways					1185:1192	biosynthetic and metabolism pathways	1157:1192	pathways	1185:1192	According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids.					
34298931	2	49	theme	tannins	289:295	arg1	abundance					276:284	a high abundance	269:284	a high abundance of tannins	269:295	It produces galls (induced by aphids) with a high abundance of tannins.					
34298931	11	50	theme	antibacterial	1635:1647	arg1	activity					1649:1656	antibacterial activity	1635:1656	antibacterial activity	1635:1656	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	6	51	theme	many	829:832	arg1	polysaccharides					834:848	many polysaccharides	829:848	many polysaccharides	829:848	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	10	52	theme	downregulated	1437:1449	arg1	genes					1451:1455	the downregulated genes	1433:1455	the downregulated genes in most subpathways	1433:1475	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	3	53	from	components	397:406	arg1	the					444:446	the	444:446	the	444:446	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	54	theme	R.	458:459	arg1	potaninii					461:469	R. potaninii	458:469	R. potaninii	458:469	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	6	55	theme	trichomes	815:823	arg1	secretion					792:800	the secretion	788:800	the secretion of glandular trichomes	788:823	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	4	56	theme	tomenta	526:532	arg1	density					491:497	the density	487:497	the density of glandular trichomes and tomenta	487:532	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	3	57	from	the	444:446	arg1	distribution					360:371	their distribution	354:371	their distribution	354:371	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	57	from	the	444:446	arg1	histology					319:327	histology	319:327	histology	319:327	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	57	from	the	444:446	arg1	components					397:406	the macromolecular components	378:406	the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii	378:469	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	57	from	the	444:446	arg1	structures					339:348	cellular structures	330:348	cellular structures	330:348	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	4	58	from	stages	617:622	arg1	sides					576:580	the adaxial and abaxial sides	552:580	the adaxial and abaxial sides of a leaf in different regions and stages of the leaf	552:634	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	8	59	theme	phenylpropanoids	1236:1251	arg1	pathways					1185:1192	biosynthetic and metabolism pathways	1157:1192	pathways	1185:1192	According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids.					
34298931	11	60	theme	pathogenesis-related	1589:1608	arg1	proteins					1610:1617	pathogenesis-related proteins	1589:1617	pathogenesis-related proteins	1589:1617	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	4	61	from	regions	605:611	arg1	sides					576:580	the adaxial and abaxial sides	552:580	the adaxial and abaxial sides of a leaf in different regions and stages of the leaf	552:634	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	10	62	theme	expressed	1331:1339	arg1	genes					1341:1345	differentially expressed genes	1316:1345	differentially expressed genes	1316:1345	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	7	63	theme	young	982:986	arg1	tissues					988:994	young tissues	982:994	young tissues	982:994	The dense glandular trichomes provide mechanical protection for young tissues; additionally, their secretion protects the young tissues from pathogens by a special chemical component.					
34298931	5	64	with	head	733:736	arg1	cells					748:752	8-15 cells	743:752	8-15 cells	743:752	The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8-15 cells.					
34298931	5	65	theme	glandular	641:649	arg1	trichomes					651:659	The glandular trichomes	637:659	The glandular trichomes on R. potaninii trees	637:681	The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8-15 cells.					
34298931	4	66	from	sides	576:580	arg1	stages					617:622	stages	617:622	stages	617:622	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	4	66	from	sides	576:580	arg1	regions					605:611	different regions	595:611	different regions	595:611	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	1	67	theme	important	193:201	arg1	species					208:214	an economically and medicinally important tree species	161:214	an economically and medicinally important tree species in China	161:223	Rhus potaninii Maxim is an economically and medicinally important tree species in China.					
34298931	1	67	theme	important	193:201	arg1	Maxim					152:156	Maxim	152:156	Maxim	152:156	Rhus potaninii Maxim is an economically and medicinally important tree species in China.					
34298931	5	68	theme	R.	664:665	arg1	trees					677:681	R. potaninii trees	664:681	R. potaninii trees	664:681	The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8-15 cells.					
34298931	8	69	theme	glycan	1197:1202	arg1	pathways					1185:1192	biosynthetic and metabolism pathways	1157:1192	pathways	1185:1192	According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids.					
34298931	12	70	from	trichomes	1784:1792	arg1	components					1741:1750	the components	1737:1750	the components of the secretion from glandular trichomes	1737:1792	To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.					
34298931	12	70	from	trichomes	1784:1792	arg1	secretion					1759:1767	the secretion	1755:1767	the secretion from glandular trichomes	1755:1792	To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.					
34298931	7	71	theme	dense	922:926	arg1	trichomes					938:946	The dense glandular trichomes	918:946	The dense glandular trichomes	918:946	The dense glandular trichomes provide mechanical protection for young tissues; additionally, their secretion protects the young tissues from pathogens by a special chemical component.					
34298931	8	72	theme	lipids	1205:1210	arg1	pathways					1185:1192	biosynthetic and metabolism pathways	1157:1192	pathways	1185:1192	According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids.					
34298931	7	73	theme	glandular	928:936	arg1	trichomes					938:946	The dense glandular trichomes	918:946	The dense glandular trichomes	918:946	The dense glandular trichomes provide mechanical protection for young tissues; additionally, their secretion protects the young tissues from pathogens by a special chemical component.					
34298931	11	74	contain	have	1630:1633	arg2	activity					1649:1656	antibacterial activity	1635:1656	antibacterial activity	1635:1656	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	11	74	contain	have	1630:1633	arg1	trichomes					1535:1543	glandular trichomes	1525:1543	glandular trichomes	1525:1543	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	3	75	from	trichomes	431:439	arg1	the					444:446	the	444:446	the	444:446	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	8	76	theme	toxic	1213:1217	arg1	acids					1225:1229	toxic amino acids	1213:1229	toxic amino acids	1213:1229	According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids.					
34298931	0	77	theme	Expression	56:65	arg1	Pattern					67:73	Gene Expression Pattern	51:73	Gene Expression Pattern	51:73	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	8	78	theme	acids	1225:1229	arg1	pathways					1185:1192	biosynthetic and metabolism pathways	1157:1192	pathways	1185:1192	According to transcriptome analysis, we found enhanced biosynthetic and metabolism pathways of glycan, lipids, toxic amino acids, and phenylpropanoids.					
34298931	11	79	located	found	1516:1520	arg2	genes					1505:1509	Some selectively expressed genes	1478:1509	Some selectively expressed genes	1478:1509	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	11	79	located	found	1516:1520	arg1	trichomes					1535:1543	glandular trichomes	1525:1543	glandular trichomes	1525:1543	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	6	80	theme	neutral	902:908	arg1	lipids					910:915	few neutral lipids	898:915	many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids	829:915	Based on staining, we found that the secretion of glandular trichomes has many polysaccharides, phenolic compounds, and acidic lipids but very few neutral lipids.					
34298931	7	81	from	pathogens	1059:1067	arg1	tissues					1046:1052	the young tissues	1036:1052	the young tissues from pathogens	1036:1067	The dense glandular trichomes provide mechanical protection for young tissues; additionally, their secretion protects the young tissues from pathogens by a special chemical component.					
34298931	0	82	theme	Trichomes	88:96	arg1	Pattern					67:73	Gene Expression Pattern	51:73	Gene Expression Pattern	51:73	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	0	82	theme	Trichomes	88:96	arg1	Composition					34:44	Chemical Composition	25:44	Chemical Composition	25:44	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	0	82	theme	Trichomes	88:96	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	0	82	theme	Trichomes	88:96	arg1	Distribution					11:22	Distribution	11:22	Distribution	11:22	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	0	83	dep	the	101:103	arg1	Leaves					105:110	Leaves	105:110	Leaves	105:110	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	5	84	theme	potaninii	667:675	arg1	trees					677:681	R. potaninii trees	664:681	R. potaninii trees	664:681	The glandular trichomes on R. potaninii trees comprise a stalk with no cellular structure and a head with 8-15 cells.					
34298931	12	85	theme	leaf	1801:1804	arg1	surface					1806:1812	the leaf surface	1797:1812	the leaf surface of R. potaninii	1797:1828	To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.					
34298931	0	86	from	Composition	34:44	arg1	the					101:103	the	101:103	the	101:103	Structure, Distribution, Chemical Composition, and Gene Expression Pattern of Glandular Trichomes on the Leaves of Rhus potaninii Maxim.					
34298931	10	87	theme	averaged	1372:1379	arg1	greater					1412:1418	greater	1412:1418	greater	1412:1418	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	10	87	theme	averaged	1372:1379	arg1	range					1381:1385	the averaged range	1368:1385	the averaged range of upregulated genes	1368:1406	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	7	88	theme	chemical	1082:1089	arg1	component					1091:1099	a special chemical component	1072:1099	a special chemical component	1072:1099	The dense glandular trichomes provide mechanical protection for young tissues; additionally, their secretion protects the young tissues from pathogens by a special chemical component.					
34298931	4	89	theme	glandular	502:510	arg1	trichomes					512:520	glandular trichomes	502:520	glandular trichomes	502:520	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	12	90	theme	glandular	1774:1782	arg1	trichomes					1784:1792	glandular trichomes	1774:1792	glandular trichomes	1774:1792	To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.					
34298931	10	91	theme	genes	1402:1406	arg1	greater					1412:1418	greater	1412:1418	greater	1412:1418	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	10	91	theme	genes	1402:1406	arg1	range					1381:1385	the averaged range	1368:1385	the averaged range of upregulated genes	1368:1406	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	3	92	theme	trichomes	431:439	arg1	distribution					360:371	their distribution	354:371	their distribution	354:371	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	92	theme	trichomes	431:439	arg1	histology					319:327	histology	319:327	histology	319:327	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	92	theme	trichomes	431:439	arg1	components					397:406	the macromolecular components	378:406	the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii	378:469	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	92	theme	trichomes	431:439	arg1	structures					339:348	cellular structures	330:348	cellular structures	330:348	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	3	93	theme	secretive	411:419	arg1	trichomes					431:439	secretive glandular trichomes	411:439	secretive glandular trichomes on the leaves of R. potaninii	411:469	Here, we discuss the histology, cellular structures and their distribution, and the macromolecular components of secretive glandular trichomes on the leaves of R. potaninii.					
34298931	10	94	from	genes	1451:1455	arg1	subpathways					1465:1475	most subpathways	1460:1475	most subpathways	1460:1475	The numbers of differentially expressed genes were large or small; the averaged range of upregulated genes was greater than that of the downregulated genes in most subpathways.					
34298931	12	95	theme	first	1717:1721	arg1	study					1723:1727	the first study	1713:1727	the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii	1713:1828	To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.					
34298931	12	95	theme	first	1717:1721	arg1	this					1705:1708	this	1705:1708	this	1705:1708	To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.					
34298931	12	96	theme	secretion	1759:1767	arg1	components					1741:1750	the components	1737:1750	the components of the secretion from glandular trichomes	1737:1792	To our knowledge, this is the first study showing the components of the secretion from glandular trichomes on the leaf surface of R. potaninii.					
34298931	4	97	theme	adaxial	556:562	arg1	sides					576:580	the adaxial and abaxial sides	552:580	the adaxial and abaxial sides of a leaf in different regions and stages of the leaf	552:634	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	11	98	theme	glandular	1525:1533	arg1	trichomes					1535:1543	glandular trichomes	1525:1543	glandular trichomes	1525:1543	Some selectively expressed genes were found in glandular trichomes, responsible for the chitinase activity and pathogenesis-related proteins, which all have antibacterial activity and serve for plant defense.					
34298931	4	99	theme	abaxial	568:574	arg1	sides					576:580	the adaxial and abaxial sides	552:580	the adaxial and abaxial sides of a leaf in different regions and stages of the leaf	552:634	A variation in the density of glandular trichomes and tomenta was found between the adaxial and abaxial sides of a leaf in different regions and stages of the leaf.					
34298931	7	100	theme	special	1074:1080	arg1	component					1091:1099	a special chemical component	1072:1099	a special chemical component	1072:1099	The dense glandular trichomes provide mechanical protection for young tissues; additionally, their secretion protects the young tissues from pathogens by a special chemical component.					
33069819	4	0	theme	scavenging	920:929	arg1	ability					931:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	1	1	from	effect	193:198	arg1	activity					238:245	the antioxidant activity	222:245	the antioxidant activity	222:245	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	1	2	theme	polysaccharides	169:183	arg1	activity					136:143	the antioxidant activity	120:143	the antioxidant activity of Porphyra haitanensis polysaccharides	120:183	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	1	2	theme	polysaccharides	169:183	arg1	effect					193:198	the effect	189:198	the effect of their structure on the antioxidant activity	189:245	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	7	3	contain	have	1285:1288	arg1	polysaccharides					1269:1283	Porphyra haitanensis polysaccharides	1248:1283	Porphyra haitanensis polysaccharides	1248:1283	Porphyra haitanensis polysaccharides have potential to develop as natural non-toxic antioxidants and may find application as the ingredients of functional foods.					
33069819	7	3	contain	have	1285:1288	arg2	potential					1290:1298	potential	1290:1298	potential to develop as natural non-toxic antioxidants	1290:1343	Porphyra haitanensis polysaccharides have potential to develop as natural non-toxic antioxidants and may find application as the ingredients of functional foods.					
33069819	4	4	theme	radical	912:918	arg1	ability					931:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	7	5	theme	haitanensis	1257:1267	arg1	polysaccharides					1269:1283	Porphyra haitanensis polysaccharides	1248:1283	Porphyra haitanensis polysaccharides	1248:1283	Porphyra haitanensis polysaccharides have potential to develop as natural non-toxic antioxidants and may find application as the ingredients of functional foods.					
33069819	3	6	theme	polysaccharide	673:686	arg1	fractions					688:696	the purified polysaccharide fractions	660:696	the purified polysaccharide fractions	660:696	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	2	7	theme	chemical	533:540	arg1	analysis					554:561	chemical composition analysis	533:561	chemical composition analysis	533:561	Three purified components including PHP1, PHP2 and PHP3 were obtained, and chemical composition analysis and structural characterization were performed.					
33069819	3	8	theme	oxidative	727:735	arg1	stress					737:742	oxidative stress	727:742	oxidative stress	727:742	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	6	9	theme	potent	1231:1236	arg1	activity					1238:1245	the most potent activity	1222:1245	the most potent activity	1222:1245	Among the three polysaccharides, PHP3 possessed the most potent activity.					
33069819	7	10	theme	Porphyra	1248:1255	arg1	polysaccharides					1269:1283	Porphyra haitanensis polysaccharides	1248:1283	Porphyra haitanensis polysaccharides	1248:1283	Porphyra haitanensis polysaccharides have potential to develop as natural non-toxic antioxidants and may find application as the ingredients of functional foods.					
33069819	5	11	theme	ROS	1022:1024	arg1	level					1026:1030	ROS level	1022:1030	ROS level	1022:1030	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	3	12	theme	macrophage	747:756	arg1	RAW264.7					758:765	macrophage RAW264.7	747:765	macrophage RAW264.7	747:765	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	4	13	theme	radical	890:896	arg1	ability					931:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	1	14	from	activity	136:143	arg1	activity					238:245	the antioxidant activity	222:245	the antioxidant activity	222:245	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	3	15	dep	in	615:616	arg1	vitro					618:622	vitro	618:622	vitro	618:622	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	2	16	theme	purified	464:471	arg1	PHP3					509:512	PHP3	509:512	PHP3	509:512	Three purified components including PHP1, PHP2 and PHP3 were obtained, and chemical composition analysis and structural characterization were performed.					
33069819	2	16	theme	purified	464:471	arg1	PHP2					500:503	PHP2	500:503	PHP2	500:503	Three purified components including PHP1, PHP2 and PHP3 were obtained, and chemical composition analysis and structural characterization were performed.					
33069819	2	16	theme	purified	464:471	arg1	PHP1					494:497	PHP1	494:497	PHP1	494:497	Three purified components including PHP1, PHP2 and PHP3 were obtained, and chemical composition analysis and structural characterization were performed.					
33069819	2	16	theme	purified	464:471	arg1	components					473:482	Three purified components	458:482	Three purified components including PHP1, PHP2 and PHP3	458:512	Three purified components including PHP1, PHP2 and PHP3 were obtained, and chemical composition analysis and structural characterization were performed.					
33069819	4	17	theme	hydroxyl	903:910	arg1	radical					912:918	hydroxyl radical	903:918	hydroxyl radical	903:918	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	5	18	theme	dismutase	1077:1085	arg1	activities					1052:1061	the activities	1048:1061	the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells	1048:1171	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	1	19	theme	DEAE-52	395:401	arg1	exchange					407:414	Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography	385:455	exchange	407:414	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	7	20	theme	foods	1403:1407	arg1	application					1358:1368	application	1358:1368	application	1358:1368	Porphyra haitanensis polysaccharides have potential to develop as natural non-toxic antioxidants and may find application as the ingredients of functional foods.					
33069819	7	20	theme	foods	1403:1407	arg1	ingredients					1377:1387	the ingredients	1373:1387	the ingredients of functional foods	1373:1407	Porphyra haitanensis polysaccharides have potential to develop as natural non-toxic antioxidants and may find application as the ingredients of functional foods.					
33069819	4	21	theme	DPPH	859:862	arg1	radical					864:870	DPPH radical	859:870	DPPH radical	859:870	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	2	22	theme	structural	567:576	arg1	characterization					578:593	structural characterization	567:593	structural characterization	567:593	Three purified components including PHP1, PHP2 and PHP3 were obtained, and chemical composition analysis and structural characterization were performed.					
33069819	4	23	theme	superoxide	873:882	arg1	radical					890:896	superoxide anion radical	873:896	superoxide anion radical	873:896	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	1	24	dep	Porphyra	148:155	arg1	haitanensis					157:167	haitanensis	157:167	haitanensis	157:167	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	1	25	theme	structure	209:217	arg1	activity					136:143	the antioxidant activity	120:143	the antioxidant activity of Porphyra haitanensis polysaccharides	120:183	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	1	25	theme	structure	209:217	arg1	effect					193:198	the effect	189:198	the effect of their structure on the antioxidant activity	189:245	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	1	26	theme	Cellulose	385:393	arg1	exchange					407:414	Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography	385:455	exchange	407:414	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	5	27	theme	MDA	1006:1008	arg1	content					1010:1016	the MDA content	1002:1016	the MDA content	1002:1016	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	7	28	theme	non-toxic	1322:1330	arg1	antioxidants					1332:1343	natural non-toxic antioxidants	1314:1343	natural non-toxic antioxidants	1314:1343	Porphyra haitanensis polysaccharides have potential to develop as natural non-toxic antioxidants and may find application as the ingredients of functional foods.					
33069819	3	29	theme	fractions	688:696	arg1	ability					708:714	their ability to relieve oxidative stress in macrophage RAW264.7	702:765	their ability to relieve oxidative stress in macrophage RAW264.7	702:765	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	3	29	theme	fractions	688:696	arg1	activity					648:655	The in vitro free radical scavenging activity	611:655	The in vitro free radical scavenging activity of the purified polysaccharide fractions	611:696	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	4	30	contain	possessed	837:845	arg1	fractions					827:835	all the three fractions	813:835	all the three fractions	813:835	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	4	30	contain	possessed	837:845	arg2	ability					931:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	4	30	contain	possessed	837:845	arg2	power					952:956	reducing power	943:956	reducing power	943:956	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	0	31	from	haitanensis	89:99	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and antioxidant activity of polysaccharides from Porphyra haitanensis.					
33069819	0	31	from	haitanensis	89:99	arg1	activity					47:54	antioxidant activity	35:54	antioxidant activity	35:54	Purification, characterization and antioxidant activity of polysaccharides from Porphyra haitanensis.					
33069819	0	31	from	haitanensis	89:99	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and antioxidant activity of polysaccharides from Porphyra haitanensis.					
33069819	5	32	from	activities	1052:1061	arg1	cells					1167:1171	H2O2-treated RAW264.7 cells	1145:1171	H2O2-treated RAW264.7 cells	1145:1171	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	7	33	theme	natural	1314:1320	arg1	antioxidants					1332:1343	natural non-toxic antioxidants	1314:1343	natural non-toxic antioxidants	1314:1343	Porphyra haitanensis polysaccharides have potential to develop as natural non-toxic antioxidants and may find application as the ingredients of functional foods.					
33069819	0	34	theme	antioxidant	35:45	arg1	activity					47:54	antioxidant activity	35:54	antioxidant activity	35:54	Purification, characterization and antioxidant activity of polysaccharides from Porphyra haitanensis.					
33069819	1	35	theme	hot	307:309	arg1	extraction					317:326	hot water extraction	307:326	hot water extraction	307:326	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	2	36	theme	composition	542:552	arg1	analysis					554:561	chemical composition analysis	533:561	chemical composition analysis	533:561	Three purified components including PHP1, PHP2 and PHP3 were obtained, and chemical composition analysis and structural characterization were performed.					
33069819	1	37	theme	ion	403:405	arg1	exchange					407:414	Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography	385:455	exchange	407:414	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	3	38	theme	free	624:627	arg1	radical					629:635	The in vitro free radical	611:635	The in vitro free radical scavenging activity of the purified polysaccharide fractions	611:696	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	1	39	theme	antioxidant	226:236	arg1	activity					238:245	the antioxidant activity	222:245	the antioxidant activity	222:245	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	0	40	theme	polysaccharides	59:73	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and antioxidant activity of polysaccharides from Porphyra haitanensis.					
33069819	0	40	theme	polysaccharides	59:73	arg1	activity					47:54	antioxidant activity	35:54	antioxidant activity	35:54	Purification, characterization and antioxidant activity of polysaccharides from Porphyra haitanensis.					
33069819	0	40	theme	polysaccharides	59:73	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and antioxidant activity of polysaccharides from Porphyra haitanensis.					
33069819	1	41	theme	water	311:315	arg1	extraction					317:326	hot water extraction	307:326	hot water extraction	307:326	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	1	42	dep	Porphyra	252:259	arg1	haitanensis					261:271	haitanensis	261:271	haitanensis	261:271	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	3	43	theme	purified	664:671	arg1	fractions					688:696	the purified polysaccharide fractions	660:696	the purified polysaccharide fractions	660:696	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	4	44	theme	anion	884:888	arg1	radical					890:896	superoxide anion radical	873:896	superoxide anion radical	873:896	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	5	45	theme	glutathione	1110:1120	arg1	GSH-Px					1134:1139	GSH-Px	1134:1139	GSH-Px	1134:1139	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	5	45	theme	glutathione	1110:1120	arg1	dismutase					1077:1085	superoxide dismutase	1066:1085	superoxide dismutase (SOD)	1066:1091	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	5	45	theme	glutathione	1110:1120	arg1	peroxidase					1122:1131	glutathione peroxidase	1110:1131	glutathione peroxidase (GSH-Px)	1110:1140	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	6	46	contain	possessed	1212:1220	arg2	activity					1238:1245	the most potent activity	1222:1245	the most potent activity	1222:1245	Among the three polysaccharides, PHP3 possessed the most potent activity.					
33069819	6	46	contain	possessed	1212:1220	arg1	PHP3					1207:1210	PHP3	1207:1210	PHP3	1207:1210	Among the three polysaccharides, PHP3 possessed the most potent activity.					
33069819	4	47	theme	reducing	943:950	arg1	power					952:956	reducing power	943:956	reducing power	943:956	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	5	48	theme	superoxide	1066:1075	arg1	SOD					1088:1090	SOD	1088:1090	SOD	1088:1090	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	5	48	theme	superoxide	1066:1075	arg1	catalase					1094:1101	catalase	1094:1101	catalase (CAT)	1094:1107	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	5	48	theme	superoxide	1066:1075	arg1	dismutase					1077:1085	superoxide dismutase	1066:1085	superoxide dismutase (SOD)	1066:1091	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	5	48	theme	superoxide	1066:1075	arg1	peroxidase					1122:1131	glutathione peroxidase	1110:1131	glutathione peroxidase (GSH-Px)	1110:1140	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	3	49	theme	in	615:616	arg1	radical					629:635	The in vitro free radical	611:635	The in vitro free radical scavenging activity of the purified polysaccharide fractions	611:696	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	5	50	theme	H2O2-treated	1145:1156	arg1	cells					1167:1171	H2O2-treated RAW264.7 cells	1145:1171	H2O2-treated RAW264.7 cells	1145:1171	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	1	51	theme	antioxidant	124:134	arg1	activity					136:143	the antioxidant activity	120:143	the antioxidant activity of Porphyra haitanensis polysaccharides	120:183	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	4	52	theme	appreciable	847:857	arg1	ability					931:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	1	53	theme	Sephadex	420:427	arg1	chromatography					442:455	Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography	385:455	chromatography	442:455	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	4	54	theme	radical	864:870	arg1	ability					931:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability	847:937	The results indicated that all the three fractions possessed appreciable DPPH radical, superoxide anion radical, and hydroxyl radical scavenging ability and reducing power.					
33069819	1	55	theme	G-100	429:433	arg1	chromatography					442:455	Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography	385:455	chromatography	442:455	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	7	56	theme	functional	1392:1401	arg1	foods					1403:1407	functional foods	1392:1407	functional foods	1392:1407	Porphyra haitanensis polysaccharides have potential to develop as natural non-toxic antioxidants and may find application as the ingredients of functional foods.					
33069819	5	57	theme	RAW264.7	1158:1165	arg1	cells					1167:1171	H2O2-treated RAW264.7 cells	1145:1171	H2O2-treated RAW264.7 cells	1145:1171	They were also found to effectively reduce the MDA content and ROS level, and to improve the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) in H2O2-treated RAW264.7 cells.					
33069819	1	58	theme	column	435:440	arg1	chromatography					442:455	Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography	385:455	chromatography	442:455	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	3	59	theme	radical	629:635	arg1	activity					648:655	The in vitro free radical scavenging activity	611:655	The in vitro free radical scavenging activity of the purified polysaccharide fractions	611:696	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	1	60	theme	Porphyra	148:155	arg1	polysaccharides					169:183	Porphyra haitanensis polysaccharides	148:183	Porphyra haitanensis polysaccharides	148:183	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	1	61	theme	alcohol	332:338	arg1	precipitation					340:352	alcohol precipitation	332:352	alcohol precipitation	332:352	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	3	62	theme	scavenging	637:646	arg1	activity					648:655	The in vitro free radical scavenging activity	611:655	The in vitro free radical scavenging activity of the purified polysaccharide fractions	611:696	The in vitro free radical scavenging activity of the purified polysaccharide fractions and their ability to relieve oxidative stress in macrophage RAW264.7 were investigated.					
33069819	1	63	theme	Porphyra	252:259	arg1	polysaccharide					273:286	the Porphyra haitanensis polysaccharide	248:286	the Porphyra haitanensis polysaccharide (PHP)	248:292	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
33069819	1	63	theme	Porphyra	252:259	arg1	PHP					289:291	PHP	289:291	PHP	289:291	In order to study the antioxidant activity of Porphyra haitanensis polysaccharides and the effect of their structure on the antioxidant activity, the Porphyra haitanensis polysaccharide (PHP), prepared by hot water extraction and alcohol precipitation, was separated and purified by Cellulose DEAE-52 ion exchange and Sephadex G-100 column chromatography.					
32867177	8	0	dep	SFPs	1499:1502	arg1	Sf-A					1516:1519	Sf-A	1516:1519	Sf-A	1516:1519	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	1	1	from	symptom	184:190	arg1	patients					204:211	diabetes patients	195:211	diabetes patients	195:211	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	3	2	theme	Sargassumfusiforme	664:681	arg1	polysaccharides					683:697	four Sargassumfusiforme polysaccharides	659:697	four Sargassumfusiforme polysaccharides	659:697	The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and four Sargassumfusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia.					
32867177	2	3	from	effects	464:470	arg1	dysbiosis					527:535	gut microbiota dysbiosis	512:535	gut microbiota dysbiosis	512:535	In this study, five polysaccharides were prepared from Sargassumfusiforme and their effects on HFD-induced fasting hypoglycemia and gut microbiota dysbiosis were investigated.					
32867177	2	3	from	effects	464:470	arg1	hypoglycemia					495:506	HFD-induced fasting hypoglycemia	475:506	HFD-induced fasting hypoglycemia	475:506	In this study, five polysaccharides were prepared from Sargassumfusiforme and their effects on HFD-induced fasting hypoglycemia and gut microbiota dysbiosis were investigated.					
32867177	0	4	theme	Microbiota	114:123	arg1	Composition					125:135	the Gut Microbiota Composition	106:135	the Gut Microbiota Composition	106:135	Sargassum fusiforme Polysaccharides Prevent High-Fat Diet-Induced Early Fasting Hypoglycemia and Regulate the Gut Microbiota Composition.					
32867177	5	5	theme	Clostridiales	1105:1117	arg1	abundance					1059:1067	the relative abundance	1046:1067	the relative abundance of Oscillospira, Mucispirillum, and Clostridiales	1046:1117	Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance of Oscillospira, Mucispirillum, and Clostridiales.					
32867177	6	6	theme	blood	1167:1171	arg1	level					1181:1185	the fasting blood glucose level	1155:1185	the fasting blood glucose level	1155:1185	Correlation analysis revealed that the fasting blood glucose level was associated with the relative abundance of Mucispinllum and Oscillospira.					
32867177	3	7	theme	fasting	781:787	arg1	hypoglycemia					789:800	early fasting hypoglycemia	775:800	early fasting hypoglycemia	775:800	The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and four Sargassumfusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia.					
32867177	0	8	theme	Gut	110:112	arg1	Composition					125:135	the Gut Microbiota Composition	106:135	the Gut Microbiota Composition	106:135	Sargassum fusiforme Polysaccharides Prevent High-Fat Diet-Induced Early Fasting Hypoglycemia and Regulate the Gut Microbiota Composition.					
32867177	8	9	from	roles	1563:1567	arg1	activity					1620:1627	the biological activity	1605:1627	the biological activity of SFPs	1605:1635	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	8	9	from	roles	1563:1567	arg1	homeostasis					1533:1543	glucose homeostasis	1525:1543	glucose homeostasis	1525:1543	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	6	10	theme	fasting	1159:1165	arg1	level					1181:1185	the fasting blood glucose level	1155:1185	the fasting blood glucose level	1155:1185	Correlation analysis revealed that the fasting blood glucose level was associated with the relative abundance of Mucispinllum and Oscillospira.					
32867177	2	11	theme	fasting	487:493	arg1	hypoglycemia					495:506	HFD-induced fasting hypoglycemia	475:506	HFD-induced fasting hypoglycemia	475:506	In this study, five polysaccharides were prepared from Sargassumfusiforme and their effects on HFD-induced fasting hypoglycemia and gut microbiota dysbiosis were investigated.					
32867177	6	12	theme	Oscillospira	1250:1261	arg1	abundance					1220:1228	the relative abundance	1207:1228	the relative abundance of Mucispinllum and Oscillospira	1207:1261	Correlation analysis revealed that the fasting blood glucose level was associated with the relative abundance of Mucispinllum and Oscillospira.					
32867177	7	13	theme	hypoglycemia	1437:1448	arg1	prediction					1415:1424	the prediction	1411:1424	the prediction of fasting hypoglycemia	1411:1448	Receiver operating characteristic analysis indicated that Mucispinllum and Oscillospira exhibited good discriminatory power (AUC = 0.745-0.833) in the prediction of fasting hypoglycemia.					
32867177	1	14	theme	common	177:182	arg1	level					166:170	A low fasting blood glucose level	138:170	A low fasting blood glucose level	138:170	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	1	14	theme	common	177:182	arg1	symptom					184:190	a common symptom	175:190	a common symptom in diabetes patients	175:211	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	2	15	theme	HFD-induced	475:485	arg1	hypoglycemia					495:506	HFD-induced fasting hypoglycemia	475:506	HFD-induced fasting hypoglycemia	475:506	In this study, five polysaccharides were prepared from Sargassumfusiforme and their effects on HFD-induced fasting hypoglycemia and gut microbiota dysbiosis were investigated.					
32867177	1	16	theme	diabetes	335:342	arg1	development					320:330	the development	316:330	the development of diabetes	316:342	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	7	17	theme	good	1362:1365	arg1	AUC					1389:1391	AUC = 0.745-0.833	1389:1405	AUC = 0.745-0.833	1389:1405	Receiver operating characteristic analysis indicated that Mucispinllum and Oscillospira exhibited good discriminatory power (AUC = 0.745-0.833) in the prediction of fasting hypoglycemia.					
32867177	7	17	theme	good	1362:1365	arg1	power					1382:1386	good discriminatory power	1362:1386	good discriminatory power (AUC = 0.745-0.833)	1362:1406	Receiver operating characteristic analysis indicated that Mucispinllum and Oscillospira exhibited good discriminatory power (AUC = 0.745-0.833) in the prediction of fasting hypoglycemia.					
32867177	0	18	theme	fusiforme	10:18	arg1	Polysaccharides					20:34	Sargassum fusiforme Polysaccharides	0:34	Sargassum fusiforme Polysaccharides	0:34	Sargassum fusiforme Polysaccharides Prevent High-Fat Diet-Induced Early Fasting Hypoglycemia and Regulate the Gut Microbiota Composition.					
32867177	8	19	theme	Mucispinllum	1572:1583	arg1	roles					1563:1567	the potential roles	1549:1567	the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs	1549:1635	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	8	19	theme	Mucispinllum	1572:1583	arg1	application					1484:1494	the novel application	1474:1494	the novel application of SFPs (especially Sf-A) in glucose homeostasis	1474:1543	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	6	20	theme	Mucispinllum	1233:1244	arg1	abundance					1220:1228	the relative abundance	1207:1228	the relative abundance of Mucispinllum and Oscillospira	1207:1261	Correlation analysis revealed that the fasting blood glucose level was associated with the relative abundance of Mucispinllum and Oscillospira.					
32867177	8	21	from	application	1484:1494	arg1	activity					1620:1627	the biological activity	1605:1627	the biological activity of SFPs	1605:1635	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	8	21	from	application	1484:1494	arg1	homeostasis					1533:1543	glucose homeostasis	1525:1543	glucose homeostasis	1525:1543	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	5	22	from	decrease	969:976	arg1	Bacteroidetes					981:993	Bacteroidetes	981:993	Bacteroidetes	981:993	Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance of Oscillospira, Mucispirillum, and Clostridiales.					
32867177	0	23	theme	Sargassum	0:8	arg1	Polysaccharides					20:34	Sargassum fusiforme Polysaccharides	0:34	Sargassum fusiforme Polysaccharides	0:34	Sargassum fusiforme Polysaccharides Prevent High-Fat Diet-Induced Early Fasting Hypoglycemia and Regulate the Gut Microbiota Composition.					
32867177	7	24	theme	fasting	1429:1435	arg1	hypoglycemia					1437:1448	fasting hypoglycemia	1429:1448	fasting hypoglycemia	1429:1448	Receiver operating characteristic analysis indicated that Mucispinllum and Oscillospira exhibited good discriminatory power (AUC = 0.745-0.833) in the prediction of fasting hypoglycemia.					
32867177	1	25	theme	diabetes	195:202	arg1	patients					204:211	diabetes patients	195:211	diabetes patients	195:211	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	8	26	theme	SFPs	1632:1635	arg1	activity					1620:1627	the biological activity	1605:1627	the biological activity of SFPs	1605:1635	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	5	27	theme	relative	1050:1057	arg1	abundance					1059:1067	the relative abundance	1046:1067	the relative abundance of Oscillospira, Mucispirillum, and Clostridiales	1046:1117	Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance of Oscillospira, Mucispirillum, and Clostridiales.					
32867177	6	28	theme	Correlation	1120:1130	arg1	analysis					1132:1139	Correlation analysis	1120:1139	Correlation analysis	1120:1139	Correlation analysis revealed that the fasting blood glucose level was associated with the relative abundance of Mucispinllum and Oscillospira.					
32867177	1	29	theme	early	269:273	arg1	stage					275:279	an early stage	266:279	an early stage	266:279	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	8	30	theme	potential	1553:1561	arg1	roles					1563:1567	the potential roles	1549:1567	the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs	1549:1635	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	3	31	dep	fed	602:604	arg1	developed					625:633	developed	625:633	developed severe hypoglycemia and four Sargassumfusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A	625:748	The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and four Sargassumfusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia.					
32867177	0	32	theme	Diet-Induced	53:64	arg1	Hypoglycemia					80:91	High-Fat Diet-Induced Early Fasting Hypoglycemia	44:91	High-Fat Diet-Induced Early Fasting Hypoglycemia	44:91	Sargassum fusiforme Polysaccharides Prevent High-Fat Diet-Induced Early Fasting Hypoglycemia and Regulate the Gut Microbiota Composition.					
32867177	7	33	theme	characteristic	1283:1296	arg1	analysis					1298:1305	characteristic analysis	1283:1305	characteristic analysis	1283:1305	Receiver operating characteristic analysis indicated that Mucispinllum and Oscillospira exhibited good discriminatory power (AUC = 0.745-0.833) in the prediction of fasting hypoglycemia.					
32867177	2	34	theme	microbiota	516:525	arg1	dysbiosis					527:535	gut microbiota dysbiosis	512:535	gut microbiota dysbiosis	512:535	In this study, five polysaccharides were prepared from Sargassumfusiforme and their effects on HFD-induced fasting hypoglycemia and gut microbiota dysbiosis were investigated.					
32867177	3	35	theme	C57BL/6J	583:590	arg1	mice					597:600	C57BL/6J male mice	583:600	C57BL/6J male mice	583:600	The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and four Sargassumfusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia.					
32867177	0	36	theme	High-Fat	44:51	arg1	Hypoglycemia					80:91	High-Fat Diet-Induced Early Fasting Hypoglycemia	44:91	High-Fat Diet-Induced Early Fasting Hypoglycemia	44:91	Sargassum fusiforme Polysaccharides Prevent High-Fat Diet-Induced Early Fasting Hypoglycemia and Regulate the Gut Microbiota Composition.					
32867177	2	37	theme	gut	512:514	arg1	dysbiosis					527:535	gut microbiota dysbiosis	512:535	gut microbiota dysbiosis	512:535	In this study, five polysaccharides were prepared from Sargassumfusiforme and their effects on HFD-induced fasting hypoglycemia and gut microbiota dysbiosis were investigated.					
32867177	3	38	theme	male	592:595	arg1	mice					597:600	C57BL/6J male mice	583:600	C57BL/6J male mice	583:600	The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and four Sargassumfusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia.					
32867177	0	39	theme	Fasting	72:78	arg1	Hypoglycemia					80:91	High-Fat Diet-Induced Early Fasting Hypoglycemia	44:91	High-Fat Diet-Induced Early Fasting Hypoglycemia	44:91	Sargassum fusiforme Polysaccharides Prevent High-Fat Diet-Induced Early Fasting Hypoglycemia and Regulate the Gut Microbiota Composition.					
32867177	8	40	theme	Oscillospira	1589:1600	arg1	roles					1563:1567	the potential roles	1549:1567	the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs	1549:1635	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	8	40	theme	Oscillospira	1589:1600	arg1	application					1484:1494	the novel application	1474:1494	the novel application of SFPs (especially Sf-A) in glucose homeostasis	1474:1543	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	4	41	theme	HFD-induced	881:891	arg1	gain					900:903	HFD-induced weight gain	881:903	HFD-induced weight gain	881:903	Sf-1 and Sf-A could also significantly prevent HFD-induced weight gain.					
32867177	1	42	theme	little	362:367	arg1	attention					369:377	little attention	362:377	little attention	362:377	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	7	43	theme	=	1393:1393	arg1	AUC					1389:1391	AUC = 0.745-0.833	1389:1405	AUC = 0.745-0.833	1389:1405	Receiver operating characteristic analysis indicated that Mucispinllum and Oscillospira exhibited good discriminatory power (AUC = 0.745-0.833) in the prediction of fasting hypoglycemia.					
32867177	7	43	theme	=	1393:1393	arg1	power					1382:1386	good discriminatory power	1362:1386	good discriminatory power (AUC = 0.745-0.833)	1362:1406	Receiver operating characteristic analysis indicated that Mucispinllum and Oscillospira exhibited good discriminatory power (AUC = 0.745-0.833) in the prediction of fasting hypoglycemia.					
32867177	5	44	theme	Oscillospira	1072:1083	arg1	abundance					1059:1067	the relative abundance	1046:1067	the relative abundance of Oscillospira, Mucispirillum, and Clostridiales	1046:1117	Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance of Oscillospira, Mucispirillum, and Clostridiales.					
32867177	0	45	theme	Early	66:70	arg1	Hypoglycemia					80:91	High-Fat Diet-Induced Early Fasting Hypoglycemia	44:91	High-Fat Diet-Induced Early Fasting Hypoglycemia	44:91	Sargassum fusiforme Polysaccharides Prevent High-Fat Diet-Induced Early Fasting Hypoglycemia and Regulate the Gut Microbiota Composition.					
32867177	8	46	theme	biological	1609:1618	arg1	activity					1620:1627	the biological activity	1605:1627	the biological activity of SFPs	1605:1635	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	5	47	theme	Mucispirillum	1086:1098	arg1	abundance					1059:1067	the relative abundance	1046:1067	the relative abundance of Oscillospira, Mucispirillum, and Clostridiales	1046:1117	Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance of Oscillospira, Mucispirillum, and Clostridiales.					
32867177	8	48	theme	SFPs	1499:1502	arg1	roles					1563:1567	the potential roles	1549:1567	the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs	1549:1635	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	8	48	theme	SFPs	1499:1502	arg1	application					1484:1494	the novel application	1474:1494	the novel application of SFPs (especially Sf-A) in glucose homeostasis	1474:1543	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	1	49	theme	low	140:142	arg1	level					166:170	A low fasting blood glucose level	138:170	A low fasting blood glucose level	138:170	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	1	49	theme	low	140:142	arg1	symptom					184:190	a common symptom	175:190	a common symptom in diabetes patients	175:211	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	5	50	theme	HFD-induced	957:967	arg1	decrease					969:976	the HFD-induced decrease	953:976	the HFD-induced decrease in Bacteroidetes	953:993	Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance of Oscillospira, Mucispirillum, and Clostridiales.					
32867177	6	51	theme	relative	1211:1218	arg1	abundance					1220:1228	the relative abundance	1207:1228	the relative abundance of Mucispinllum and Oscillospira	1207:1261	Correlation analysis revealed that the fasting blood glucose level was associated with the relative abundance of Mucispinllum and Oscillospira.					
32867177	1	52	theme	fasting	144:150	arg1	level					166:170	A low fasting blood glucose level	138:170	A low fasting blood glucose level	138:170	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	1	52	theme	fasting	144:150	arg1	symptom					184:190	a common symptom	175:190	a common symptom in diabetes patients	175:211	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	4	53	theme	weight	893:898	arg1	gain					900:903	HFD-induced weight gain	881:903	HFD-induced weight gain	881:903	Sf-1 and Sf-A could also significantly prevent HFD-induced weight gain.					
32867177	3	54	theme	severe	635:640	arg1	hypoglycemia					642:653	severe hypoglycemia	635:653	severe hypoglycemia	635:653	The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and four Sargassumfusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia.					
32867177	3	54	theme	severe	635:640	arg1	SFPs					700:703	SFPs	700:703	SFPs	700:703	The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and four Sargassumfusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia.					
32867177	1	55	theme	blood	152:156	arg1	level					166:170	A low fasting blood glucose level	138:170	A low fasting blood glucose level	138:170	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	1	55	theme	blood	152:156	arg1	symptom					184:190	a common symptom	175:190	a common symptom in diabetes patients	175:211	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	3	56	theme	early	775:779	arg1	hypoglycemia					789:800	early fasting hypoglycemia	775:800	early fasting hypoglycemia	775:800	The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and four Sargassumfusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia.					
32867177	8	57	theme	glucose	1525:1531	arg1	homeostasis					1533:1543	glucose homeostasis	1525:1543	glucose homeostasis	1525:1543	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	5	58	theme	considerable	1020:1031	arg1	influence					1033:1041	a considerable influence	1018:1041	a considerable influence	1018:1041	Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance of Oscillospira, Mucispirillum, and Clostridiales.					
32867177	7	59	theme	discriminatory	1367:1380	arg1	AUC					1389:1391	AUC = 0.745-0.833	1389:1405	AUC = 0.745-0.833	1389:1405	Receiver operating characteristic analysis indicated that Mucispinllum and Oscillospira exhibited good discriminatory power (AUC = 0.745-0.833) in the prediction of fasting hypoglycemia.					
32867177	7	59	theme	discriminatory	1367:1380	arg1	power					1382:1386	good discriminatory power	1362:1386	good discriminatory power (AUC = 0.745-0.833)	1362:1406	Receiver operating characteristic analysis indicated that Mucispinllum and Oscillospira exhibited good discriminatory power (AUC = 0.745-0.833) in the prediction of fasting hypoglycemia.					
32867177	8	60	theme	novel	1478:1482	arg1	application					1484:1494	the novel application	1474:1494	the novel application of SFPs (especially Sf-A) in glucose homeostasis	1474:1543	Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.					
32867177	1	61	theme	glucose	158:164	arg1	level					166:170	A low fasting blood glucose level	138:170	A low fasting blood glucose level	138:170	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	1	61	theme	glucose	158:164	arg1	symptom					184:190	a common symptom	175:190	a common symptom in diabetes patients	175:211	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	1	62	theme	high-fat	235:242	arg1	HFD					250:252	HFD	250:252	HFD	250:252	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	1	62	theme	high-fat	235:242	arg1	diet					244:247	high-fat diet	235:247	high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes	235:342	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	5	63	contain	had	1014:1016	arg1	SFPs					1009:1012	all five SFPs	1000:1012	all five SFPs	1000:1012	Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance of Oscillospira, Mucispirillum, and Clostridiales.					
32867177	5	63	contain	had	1014:1016	arg2	influence					1033:1041	a considerable influence	1018:1041	a considerable influence	1018:1041	Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance of Oscillospira, Mucispirillum, and Clostridiales.					
32867177	1	64	theme	important	297:305	arg1	roles					307:311	important roles	297:311	important roles	297:311	A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention.					
32867177	6	65	theme	glucose	1173:1179	arg1	level					1181:1185	the fasting blood glucose level	1155:1185	the fasting blood glucose level	1155:1185	Correlation analysis revealed that the fasting blood glucose level was associated with the relative abundance of Mucispinllum and Oscillospira.					
32867177	3	66	dep	developed	625:633	arg1	prevented					765:773	prevented	765:773	significantly prevented early fasting hypoglycemia without inducing hyperglycemia	751:831	The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and four Sargassumfusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia.					
33964361	3	0	theme	potential	544:552	arg1	mechanisms					554:563	potential mechanisms	544:563	potential mechanisms	544:563	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	9	1	theme	OLP	1565:1567	arg1	mechanisms					1551:1560	the possible mechanisms	1538:1560	the possible mechanisms of OLP to treat CKD	1538:1580	Moreover, the expression levels of a series of cytokines related to the inflammation, apoptosis and fibrosis were analyzed to explore the possible mechanisms of OLP to treat CKD.					
33964361	3	2	from	mechanisms	554:563	arg1	treatment					613:621	the treatment	609:621	the treatment of chronic kidney disease (CKD)	609:653	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	11	3	theme	main	1727:1730	arg1	linkage					1732:1738	the main linkage	1723:1738	the main linkage	1723:1738	There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage.					
33964361	11	3	theme	main	1727:1730	arg1	-Glcp-					1710:1715	→4)-Glcp-	1707:1715	→4)-Glcp-(1→	1707:1718	There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage.					
33964361	1	4	theme	urinary	309:315	arg1	diseases					317:324	urinary diseases	309:324	urinary diseases	309:324	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	12	5	theme	OLP	1875:1877	arg1	treatment					1862:1870	the treatment	1858:1870	the treatment of OLP	1858:1877	OLP could significantly attenuate CKD in mice and the tubulointerstitial damage was recovered to almost normal after the treatment of OLP.					
33964361	14	6	theme	renal	2268:2272	arg1	cells					2274:2278	renal cells	2268:2278	renal cells	2268:2278	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	3	7	theme	lanpingensis	571:582	arg1	polysaccharides					584:598	O. lanpingensis polysaccharides	568:598	O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD)	568:653	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	3	7	theme	lanpingensis	571:582	arg1	OLP					601:603	OLP	601:603	OLP	601:603	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	15	8	theme	monosaccharides	2419:2433	arg1	kinds					2410:2414	three kinds	2404:2414	three kinds of monosaccharides	2404:2433	CONCLUSIONS OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide.					
33964361	1	9	theme	Eastern	229:235	arg1	Himalayas					237:245	Eastern Himalayas	229:245	Eastern Himalayas	229:245	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	4	10	theme	MATERIALS	695:703	arg1	analysis					729:736	MATERIALS AND METHODS Methylation analysis	695:736	MATERIALS AND METHODS Methylation analysis	695:736	MATERIALS AND METHODS Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP.					
33964361	11	11	from	kinds	1658:1662	arg1	OLP					1690:1692	OLP	1690:1692	OLP	1690:1692	There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage.					
33964361	4	12	dep	composition	781:791	arg1	the					762:764	the	762:764	the	762:764	MATERIALS AND METHODS Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP.					
33964361	1	13	used	used	263:266	arg2	ethno-medicine					199:212	a traditional ethno-medicine	185:212	a traditional ethno-medicine	185:212	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	1	13	used	used	263:266	arg2	lanpingensis					151:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis	105:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis)	105:180	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	8	14	theme	oxygen	1239:1244	arg1	ROS					1255:1257	ROS	1255:1257	ROS	1255:1257	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	14	theme	oxygen	1239:1244	arg1	species					1246:1252	reactive oxygen species	1230:1252	reactive oxygen species (ROS)	1230:1258	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	3	15	theme	kidney	634:639	arg1	CKD					650:652	CKD	650:652	CKD	650:652	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	3	15	theme	kidney	634:639	arg1	disease					641:647	chronic kidney disease	626:647	chronic kidney disease (CKD)	626:653	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	5	16	theme	model	878:882	arg1	groups					938:943	OLP groups	934:943	OLP groups	934:943	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	5	16	theme	model	878:882	arg1	group					884:888	CKD model group	874:888	CKD model group	874:888	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	6	17	theme	mouse	954:958	arg1	model					960:964	The CKD mouse model	946:964	The CKD mouse model	946:964	The CKD mouse model was established by the adenine gavage.					
33964361	13	18	from	levels	1919:1924	arg1	groups					1963:1968	OLP treatment groups	1949:1968	OLP treatment groups	1949:1968	Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment.					
33964361	7	19	theme	histological	1009:1020	arg1	changes					1022:1028	The histological changes	1005:1028	The histological changes of renal tissue	1005:1044	The histological changes of renal tissue were observed by Hematoxylin-eosin and Masson staining.					
33964361	5	20	theme	OLP	934:936	arg1	groups					938:943	OLP groups	934:943	OLP groups	934:943	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	8	21	theme	CKD	1392:1394	arg1	alleviation					1377:1387	the alleviation	1373:1387	the alleviation of CKD by OLP	1373:1401	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	16	22	theme	oxidative	2556:2564	arg1	stress					2566:2571	the oxidative stress	2552:2571	the oxidative stress	2552:2571	OLP could ameliorate CKD in mice by declining the oxidative stress, inflammation, apoptosis and fibrosis in the kidneys.					
33964361	14	23	theme	tissues	2129:2135	arg1	stress					2109:2114	the oxidative stress	2095:2114	the oxidative stress of the renal tissues	2095:2135	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	16	24	from	CKD	2527:2529	arg1	mice					2534:2537	mice	2534:2537	mice	2534:2537	OLP could ameliorate CKD in mice by declining the oxidative stress, inflammation, apoptosis and fibrosis in the kidneys.					
33964361	9	25	theme	expression	1418:1427	arg1	levels					1429:1434	the expression levels	1414:1434	the expression levels of a series of cytokines related to the inflammation, apoptosis and fibrosis	1414:1511	Moreover, the expression levels of a series of cytokines related to the inflammation, apoptosis and fibrosis were analyzed to explore the possible mechanisms of OLP to treat CKD.					
33964361	15	26	from	linkage	2475:2481	arg1	polysaccharide					2490:2503	the polysaccharide	2486:2503	the polysaccharide	2486:2503	CONCLUSIONS OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide.					
33964361	8	27	theme	superoxide	1261:1270	arg1	SOD					1283:1285	SOD	1283:1285	SOD	1283:1285	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	27	theme	superoxide	1261:1270	arg1	dismutase					1272:1280	superoxide dismutase	1261:1280	superoxide dismutase (SOD)	1261:1286	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	0	28	theme	MAPK/NF-κB	85:94	arg1	pathway					96:102	MAPK/NF-κB pathway	85:102	MAPK/NF-κB pathway	85:102	Ophiocordyceps lanpingensis polysaccharides alleviate chronic kidney disease through MAPK/NF-κB pathway.					
33964361	1	29	theme	traditional	187:197	arg1	lanpingensis					151:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis	105:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis)	105:180	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	1	29	theme	traditional	187:197	arg1	ethno-medicine					199:212	a traditional ethno-medicine	185:212	a traditional ethno-medicine	185:212	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	11	30	theme	glycosidic	1667:1676	arg1	linkages					1678:1685	glycosidic linkages	1667:1685	glycosidic linkages in OLP	1667:1692	There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage.					
33964361	15	31	theme	main	2459:2462	arg1	-Glcp-					2442:2447	-Glcp-	2442:2447	-Glcp-(1→	2442:2450	CONCLUSIONS OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide.					
33964361	15	31	theme	main	2459:2462	arg1	linkage					2475:2481	the main glycosidic linkage	2455:2481	the main glycosidic linkage in the polysaccharide	2455:2503	CONCLUSIONS OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide.					
33964361	9	32	theme	series	1441:1446	arg1	levels					1429:1434	the expression levels	1414:1434	the expression levels of a series of cytokines related to the inflammation, apoptosis and fibrosis	1414:1511	Moreover, the expression levels of a series of cytokines related to the inflammation, apoptosis and fibrosis were analyzed to explore the possible mechanisms of OLP to treat CKD.					
33964361	14	33	theme	TGF-β1	2365:2370	arg1	expression					2351:2360	the expression	2347:2360	the expression of TGF-β1	2347:2370	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	14	34	theme	pro-inflammatory	2172:2187	arg1	factors					2189:2195	pro-inflammatory factors	2172:2195	pro-inflammatory factors	2172:2195	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	13	35	theme	CKD	1898:1900	arg1	group					1908:1912	the CKD model group	1894:1912	the CKD model group	1894:1912	Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment.					
33964361	4	36	from	linkages	808:815	arg1	OLP					820:822	OLP	820:822	OLP	820:822	MATERIALS AND METHODS Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP.					
33964361	9	37	theme	cytokines	1451:1459	arg1	series					1441:1446	a series	1439:1446	a series of cytokines related to the inflammation, apoptosis and fibrosis	1439:1511	Moreover, the expression levels of a series of cytokines related to the inflammation, apoptosis and fibrosis were analyzed to explore the possible mechanisms of OLP to treat CKD.					
33964361	6	38	theme	adenine	989:995	arg1	gavage					997:1002	the adenine gavage	985:1002	the adenine gavage	985:1002	The CKD mouse model was established by the adenine gavage.					
33964361	8	39	theme	glutathione	1289:1299	arg1	GSH-PX					1313:1318	GSH-PX	1313:1318	GSH-PX	1313:1318	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	39	theme	glutathione	1289:1299	arg1	peroxidase					1301:1310	glutathione peroxidase	1289:1310	glutathione peroxidase (GSH-PX)	1289:1319	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	17	40	theme	potential	2669:2677	arg1	application					2679:2689	the potential application	2665:2689	the potential application of OLP in alleviating CKD	2665:2715	The study provided some evidences for the potential application of OLP in alleviating CKD.					
33964361	1	41	theme	ETHNOPHARMACOLOGICAL	105:124	arg1	ethno-medicine					199:212	a traditional ethno-medicine	185:212	a traditional ethno-medicine	185:212	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	1	41	theme	ETHNOPHARMACOLOGICAL	105:124	arg1	lanpingensis					151:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis	105:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis)	105:180	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	1	41	theme	ETHNOPHARMACOLOGICAL	105:124	arg1	lanpingensis					168:179	O. lanpingensis	165:179	O. lanpingensis	165:179	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	4	42	theme	monosaccharide	766:779	arg1	composition					781:791	monosaccharide composition	766:791	monosaccharide composition	766:791	MATERIALS AND METHODS Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP.					
33964361	2	43	theme	such	435:438	arg1	medication					440:449	such medication	435:449	such medication	435:449	However, the corresponding active components and related pharmacological mechanism of such medication are not clear yet.					
33964361	0	44	theme	Ophiocordyceps	0:13	arg1	polysaccharides					28:42	Ophiocordyceps lanpingensis polysaccharides	0:42	Ophiocordyceps lanpingensis polysaccharides	0:42	Ophiocordyceps lanpingensis polysaccharides alleviate chronic kidney disease through MAPK/NF-κB pathway.					
33964361	13	45	theme	Ca	2026:2027	arg1	levels					2008:2013	the levels	2004:2013	the levels of SOD and Ca	2004:2027	Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment.					
33964361	1	46	theme	Ophiocordyceps	136:149	arg1	ethno-medicine					199:212	a traditional ethno-medicine	185:212	a traditional ethno-medicine	185:212	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	1	46	theme	Ophiocordyceps	136:149	arg1	lanpingensis					151:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis	105:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis)	105:180	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	1	46	theme	Ophiocordyceps	136:149	arg1	lanpingensis					168:179	O. lanpingensis	165:179	O. lanpingensis	165:179	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	2	47	dep	components	383:392	arg1	the					358:360	the	358:360	the	358:360	However, the corresponding active components and related pharmacological mechanism of such medication are not clear yet.					
33964361	10	48	theme	RESULTS	1583:1589	arg1	OLP					1591:1593	RESULTS OLP	1583:1593	RESULTS OLP	1583:1593	RESULTS OLP is composed of three kinds of monosaccharides.					
33964361	5	49	theme	control	859:865	arg1	group					867:871	the control group	855:871	the control group	855:871	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	5	49	theme	control	859:865	arg1	groups					938:943	OLP groups	934:943	OLP groups	934:943	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	4	50	theme	METHODS	709:715	arg1	analysis					729:736	MATERIALS AND METHODS Methylation analysis	695:736	MATERIALS AND METHODS Methylation analysis	695:736	MATERIALS AND METHODS Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP.					
33964361	3	51	theme	previous	668:675	arg1	results					686:692	our previous research results	664:692	our previous research results	664:692	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	0	52	theme	chronic	54:60	arg1	disease					69:75	chronic kidney disease	54:75	chronic kidney disease	54:75	Ophiocordyceps lanpingensis polysaccharides alleviate chronic kidney disease through MAPK/NF-κB pathway.					
33964361	1	53	theme	years	342:346	arg1	hundreds					330:337	hundreds	330:337	hundreds of years	330:346	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	15	54	dep	-Glcp-	2442:2447	arg1	1→					2449:2450	1→	2449:2450	1→	2449:2450	CONCLUSIONS OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide.					
33964361	13	55	theme	SOD	2018:2020	arg1	levels					2008:2013	the levels	2004:2013	the levels of SOD and Ca	2004:2027	Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment.					
33964361	14	56	theme	renal	2313:2317	arg1	fibrosis					2319:2326	the renal fibrosis	2309:2326	the renal fibrosis	2309:2326	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	2	57	theme	corresponding	362:374	arg1	components					383:392	corresponding active components	362:392	corresponding active components	362:392	However, the corresponding active components and related pharmacological mechanism of such medication are not clear yet.					
33964361	8	58	theme	urea	1142:1145	arg1	BUN					1157:1159	BUN	1157:1159	BUN	1157:1159	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	58	theme	urea	1142:1145	arg1	nitrogen					1147:1154	blood urea nitrogen	1136:1154	blood urea nitrogen (BUN)	1136:1160	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	10	59	theme	monosaccharides	1625:1639	arg1	kinds					1616:1620	three kinds	1610:1620	three kinds of monosaccharides	1610:1639	RESULTS OLP is composed of three kinds of monosaccharides.					
33964361	9	60	theme	possible	1542:1549	arg1	mechanisms					1551:1560	the possible mechanisms	1538:1560	the possible mechanisms of OLP to treat CKD	1538:1580	Moreover, the expression levels of a series of cytokines related to the inflammation, apoptosis and fibrosis were analyzed to explore the possible mechanisms of OLP to treat CKD.					
33964361	14	61	theme	TLR4-mediated	2205:2217	arg1	MAPK					2219:2222	TLR4-mediated MAPK and NF-κB pathway	2205:2240	MAPK	2219:2222	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	2	62	theme	related	398:404	arg1	mechanism					422:430	related pharmacological mechanism	398:430	related pharmacological mechanism	398:430	However, the corresponding active components and related pharmacological mechanism of such medication are not clear yet.					
33964361	13	63	theme	treatment	1953:1961	arg1	groups					1963:1968	OLP treatment groups	1949:1968	OLP treatment groups	1949:1968	Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment.					
33964361	14	64	theme	MAPK	2283:2286	arg1	pathway					2288:2294	MAPK pathway	2283:2294	MAPK pathway	2283:2294	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	14	65	theme	factors	2189:2195	arg1	levels					2162:2167	the expression levels	2147:2167	the expression levels of pro-inflammatory factors	2147:2195	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	3	66	theme	polysaccharides	584:598	arg1	effects					532:538	effects	532:538	effects	532:538	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	3	66	theme	polysaccharides	584:598	arg1	mechanisms					554:563	potential mechanisms	544:563	potential mechanisms	544:563	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	8	67	theme	serum	1163:1167	arg1	creatinine					1169:1178	serum creatinine	1163:1178	serum creatinine (Scr)	1163:1184	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	67	theme	serum	1163:1167	arg1	Scr					1181:1183	Scr	1181:1183	Scr	1181:1183	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	14	68	theme	cells	2274:2278	arg1	apoptosis					2255:2263	the apoptosis	2251:2263	the apoptosis of renal cells	2251:2278	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	3	69	theme	O.	568:569	arg1	polysaccharides					584:598	O. lanpingensis polysaccharides	568:598	O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD)	568:653	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	3	69	theme	O.	568:569	arg1	OLP					601:603	OLP	601:603	OLP	601:603	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	11	70	from	OLP	1690:1692	arg1	kinds					1658:1662	eight kinds	1652:1662	eight kinds	1652:1662	There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage.					
33964361	8	71	theme	plasma	1209:1214	arg1	Ca					1225:1226	Ca	1225:1226	Ca	1225:1226	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	71	theme	plasma	1209:1214	arg1	calcium					1216:1222	plasma calcium	1209:1222	plasma calcium (Ca)	1209:1227	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	14	72	theme	expression	2151:2160	arg1	levels					2162:2167	the expression levels	2147:2167	the expression levels of pro-inflammatory factors	2147:2195	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	5	73	theme	losartan	891:898	arg1	groups					938:943	OLP groups	934:943	OLP groups	934:943	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	5	73	theme	losartan	891:898	arg1	group					900:904	losartan group	891:904	losartan group	891:904	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	7	74	theme	Masson	1085:1090	arg1	staining					1092:1099	Masson staining	1085:1099	Masson staining	1085:1099	The histological changes of renal tissue were observed by Hematoxylin-eosin and Masson staining.					
33964361	3	75	from	effects	532:538	arg1	treatment					613:621	the treatment	609:621	the treatment of chronic kidney disease (CKD)	609:653	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	8	76	theme	serum	1187:1191	arg1	P					1205:1205	P	1205:1205	P	1205:1205	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	76	theme	serum	1187:1191	arg1	phosphorus					1193:1202	serum phosphorus	1187:1202	serum phosphorus (P)	1187:1206	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	14	77	theme	oxidative	2099:2107	arg1	stress					2109:2114	the oxidative stress	2095:2114	the oxidative stress of the renal tissues	2095:2135	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	3	78	theme	chronic	626:632	arg1	CKD					650:652	CKD	650:652	CKD	650:652	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	3	78	theme	chronic	626:632	arg1	disease					641:647	chronic kidney disease	626:647	chronic kidney disease (CKD)	626:653	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	5	79	theme	CKD	874:876	arg1	groups					938:943	OLP groups	934:943	OLP groups	934:943	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	5	79	theme	CKD	874:876	arg1	group					884:888	CKD model group	874:888	CKD model group	874:888	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	12	80	theme	tubulointerstitial	1795:1812	arg1	damage					1814:1819	the tubulointerstitial damage	1791:1819	the tubulointerstitial damage	1791:1819	OLP could significantly attenuate CKD in mice and the tubulointerstitial damage was recovered to almost normal after the treatment of OLP.					
33964361	11	81	from	linkages	1678:1685	arg1	OLP					1690:1692	OLP	1690:1692	OLP	1690:1692	There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage.					
33964361	8	82	theme	reactive	1230:1237	arg1	ROS					1255:1257	ROS	1255:1257	ROS	1255:1257	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	82	theme	reactive	1230:1237	arg1	species					1246:1252	reactive oxygen species	1230:1252	reactive oxygen species (ROS)	1230:1258	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	13	83	theme	OLP	1949:1951	arg1	groups					1963:1968	OLP treatment groups	1949:1968	OLP treatment groups	1949:1968	Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment.					
33964361	7	84	theme	renal	1033:1037	arg1	tissue					1039:1044	renal tissue	1033:1044	renal tissue	1033:1044	The histological changes of renal tissue were observed by Hematoxylin-eosin and Masson staining.					
33964361	1	85	theme	O.	165:166	arg1	lanpingensis					151:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis	105:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis)	105:180	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	1	85	theme	O.	165:166	arg1	lanpingensis					168:179	O. lanpingensis	165:179	O. lanpingensis	165:179	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	6	86	theme	CKD	950:952	arg1	model					960:964	The CKD mouse model	946:964	The CKD mouse model	946:964	The CKD mouse model was established by the adenine gavage.					
33964361	7	87	theme	tissue	1039:1044	arg1	changes					1022:1028	The histological changes	1005:1028	The histological changes of renal tissue	1005:1044	The histological changes of renal tissue were observed by Hematoxylin-eosin and Masson staining.					
33964361	5	88	theme	groups	938:943	arg1	group					867:871	the control group	855:871	the control group	855:871	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	5	88	theme	groups	938:943	arg1	doses					925:929	three different doses	910:929	three different doses of OLP groups	910:943	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	5	88	theme	groups	938:943	arg1	groups					938:943	OLP groups	934:943	OLP groups	934:943	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	5	88	theme	groups	938:943	arg1	group					884:888	CKD model group	874:888	CKD model group	874:888	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	5	88	theme	groups	938:943	arg1	group					900:904	losartan group	891:904	losartan group	891:904	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	14	89	theme	renal	2123:2127	arg1	tissues					2129:2135	the renal tissues	2119:2135	the renal tissues	2119:2135	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	15	90	theme	glycosidic	2464:2473	arg1	-Glcp-					2442:2447	-Glcp-	2442:2447	-Glcp-(1→	2442:2450	CONCLUSIONS OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide.					
33964361	15	90	theme	glycosidic	2464:2473	arg1	linkage					2475:2481	the main glycosidic linkage	2455:2481	the main glycosidic linkage in the polysaccharide	2455:2503	CONCLUSIONS OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide.					
33964361	3	91	theme	disease	641:647	arg1	treatment					613:621	the treatment	609:621	the treatment of chronic kidney disease (CKD)	609:653	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	13	92	theme	OLP	2050:2052	arg1	treatment					2054:2062	OLP treatment	2050:2062	OLP treatment	2050:2062	Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment.					
33964361	5	93	theme	different	916:923	arg1	doses					925:929	three different doses	910:929	three different doses of OLP groups	910:943	The animals were divided into the control group, CKD model group, losartan group and three different doses of OLP groups.					
33964361	12	94	from	CKD	1775:1777	arg1	mice					1782:1785	mice	1782:1785	mice	1782:1785	OLP could significantly attenuate CKD in mice and the tubulointerstitial damage was recovered to almost normal after the treatment of OLP.					
33964361	4	95	theme	Methylation	717:727	arg1	analysis					729:736	MATERIALS AND METHODS Methylation analysis	695:736	MATERIALS AND METHODS Methylation analysis	695:736	MATERIALS AND METHODS Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP.					
33964361	3	96	from	polysaccharides	584:598	arg1	treatment					613:621	the treatment	609:621	the treatment of chronic kidney disease (CKD)	609:653	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	11	97	theme	linkages	1678:1685	arg1	kinds					1658:1662	eight kinds	1652:1662	eight kinds	1652:1662	There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage.					
33964361	13	98	theme	Scr	1929:1931	arg1	levels					1919:1924	the levels	1915:1924	the levels of Scr, BUN, MDA, P in OLP treatment groups	1915:1968	Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment.					
33964361	4	99	theme	glycosidic	797:806	arg1	linkages					808:815	glycosidic linkages	797:815	glycosidic linkages	797:815	MATERIALS AND METHODS Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP.					
33964361	13	100	theme	model	1902:1906	arg1	group					1908:1912	the CKD model group	1894:1912	the CKD model group	1894:1912	Compared with the CKD model group, the levels of Scr, BUN, MDA, P in OLP treatment groups were significantly decreased; and the levels of SOD and Ca were increased after OLP treatment.					
33964361	4	101	used	used	742:745	arg2	analysis					729:736	MATERIALS AND METHODS Methylation analysis	695:736	MATERIALS AND METHODS Methylation analysis	695:736	MATERIALS AND METHODS Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP.					
33964361	9	102	theme	related	1461:1467	arg1	cytokines					1451:1459	cytokines	1451:1459	cytokines related to the inflammation, apoptosis and fibrosis	1451:1511	Moreover, the expression levels of a series of cytokines related to the inflammation, apoptosis and fibrosis were analyzed to explore the possible mechanisms of OLP to treat CKD.					
33964361	3	103	theme	STUDY	482:486	arg1	AIMS					470:473	AIMS	470:473	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.	470:693	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	0	104	theme	lanpingensis	15:26	arg1	polysaccharides					28:42	Ophiocordyceps lanpingensis polysaccharides	0:42	Ophiocordyceps lanpingensis polysaccharides	0:42	Ophiocordyceps lanpingensis polysaccharides alleviate chronic kidney disease through MAPK/NF-κB pathway.					
33964361	11	105	dep	-Glcp-	1710:1715	arg1	→4					1707:1708	→4	1707:1708	→4	1707:1708	There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage.					
33964361	11	105	dep	-Glcp-	1710:1715	arg1	1→					1717:1718	1→	1717:1718	1→	1717:1718	There are eight kinds of glycosidic linkages in OLP, among which →4)-Glcp-(1→ is the main linkage.					
33964361	4	106	from	composition	781:791	arg1	OLP					820:822	OLP	820:822	OLP	820:822	MATERIALS AND METHODS Methylation analysis was used to investigate the monosaccharide composition and glycosidic linkages in OLP.					
33964361	1	107	theme	RELEVANCE	126:134	arg1	ethno-medicine					199:212	a traditional ethno-medicine	185:212	a traditional ethno-medicine	185:212	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	1	107	theme	RELEVANCE	126:134	arg1	lanpingensis					151:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis	105:162	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis)	105:180	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	1	107	theme	RELEVANCE	126:134	arg1	lanpingensis					168:179	O. lanpingensis	165:179	O. lanpingensis	165:179	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	3	108	from	treatment	613:621	arg1	effects					532:538	effects	532:538	effects	532:538	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	3	108	from	treatment	613:621	arg1	mechanisms					554:563	potential mechanisms	544:563	potential mechanisms	544:563	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	17	109	theme	OLP	2694:2696	arg1	application					2679:2689	the potential application	2665:2689	the potential application of OLP in alleviating CKD	2665:2715	The study provided some evidences for the potential application of OLP in alleviating CKD.					
33964361	8	110	theme	Biochemical	1102:1112	arg1	phosphorus					1193:1202	serum phosphorus	1187:1202	serum phosphorus (P)	1187:1206	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	110	theme	Biochemical	1102:1112	arg1	dismutase					1272:1280	superoxide dismutase	1261:1280	superoxide dismutase (SOD)	1261:1286	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	110	theme	Biochemical	1102:1112	arg1	calcium					1216:1222	plasma calcium	1209:1222	plasma calcium (Ca)	1209:1227	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	110	theme	Biochemical	1102:1112	arg1	peroxidase					1301:1310	glutathione peroxidase	1289:1310	glutathione peroxidase (GSH-PX)	1289:1319	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	110	theme	Biochemical	1102:1112	arg1	nitrogen					1147:1154	blood urea nitrogen	1136:1154	blood urea nitrogen (BUN)	1136:1160	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	110	theme	Biochemical	1102:1112	arg1	creatinine					1169:1178	serum creatinine	1163:1178	serum creatinine (Scr)	1163:1184	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	110	theme	Biochemical	1102:1112	arg1	species					1246:1252	reactive oxygen species	1230:1252	reactive oxygen species (ROS)	1230:1258	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	110	theme	Biochemical	1102:1112	arg1	indicators					1114:1123	Biochemical indicators	1102:1123	Biochemical indicators	1102:1123	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	110	theme	Biochemical	1102:1112	arg1	malondialdehyde					1325:1339	malondialdehyde	1325:1339	malondialdehyde (MDA)	1325:1345	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	0	111	theme	kidney	62:67	arg1	disease					69:75	chronic kidney disease	54:75	chronic kidney disease	54:75	Ophiocordyceps lanpingensis polysaccharides alleviate chronic kidney disease through MAPK/NF-κB pathway.					
33964361	3	112	dep	AIMS	470:473	arg1	performed					503:511	performed	503:511	was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results	499:692	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	15	113	dep	linkage	2475:2481	arg1	→4					2439:2440	→4	2439:2440	→4	2439:2440	CONCLUSIONS OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide.					
33964361	8	114	theme	blood	1136:1140	arg1	BUN					1157:1159	BUN	1157:1159	BUN	1157:1159	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	8	114	theme	blood	1136:1140	arg1	nitrogen					1147:1154	blood urea nitrogen	1136:1154	blood urea nitrogen (BUN)	1136:1160	Biochemical indicators, including blood urea nitrogen (BUN), serum creatinine (Scr), serum phosphorus (P), plasma calcium (Ca), reactive oxygen species (ROS), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) were measured to evaluate the alleviation of CKD by OLP.					
33964361	2	115	theme	active	376:381	arg1	components					383:392	corresponding active components	362:392	corresponding active components	362:392	However, the corresponding active components and related pharmacological mechanism of such medication are not clear yet.					
33964361	3	116	dep	effects	532:538	arg1	the					528:530	the	528:530	the	528:530	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	3	117	theme	research	677:684	arg1	results					686:692	our previous research results	664:692	our previous research results	664:692	AIMS OF THE STUDY This study was performed to investigate the effects and potential mechanisms of O. lanpingensis polysaccharides (OLP) in the treatment of chronic kidney disease (CKD) based on our previous research results.					
33964361	14	118	theme	NF-κB	2228:2232	arg1	pathway					2234:2240	TLR4-mediated MAPK and NF-κB pathway	2205:2240	pathway	2234:2240	Furthermore, OLP could reduce the oxidative stress of the renal tissues, decrease the expression levels of pro-inflammatory factors through TLR4-mediated MAPK and NF-κB pathway, inhibit the apoptosis of renal cells by MAPK pathway, and relieve the renal fibrosis by down-regulating the expression of TGF-β1.					
33964361	15	119	theme	CONCLUSIONS	2373:2383	arg1	OLP					2385:2387	CONCLUSIONS OLP	2373:2387	CONCLUSIONS OLP	2373:2387	CONCLUSIONS OLP is composed of three kinds of monosaccharides and →4)-Glcp-(1→ is the main glycosidic linkage in the polysaccharide.					
33964361	1	120	theme	local	271:275	arg1	minorities					277:286	local minorities	271:286	local minorities	271:286	ETHNOPHARMACOLOGICAL RELEVANCE Ophiocordyceps lanpingensis (O. lanpingensis) is a traditional ethno-medicine distributed in Eastern Himalayas, which has been used by local minorities to prevent and treat urinary diseases for hundreds of years.					
33964361	2	121	theme	medication	440:449	arg1	components					383:392	corresponding active components	362:392	corresponding active components	362:392	However, the corresponding active components and related pharmacological mechanism of such medication are not clear yet.					
33964361	2	121	theme	medication	440:449	arg1	mechanism					422:430	related pharmacological mechanism	398:430	related pharmacological mechanism	398:430	However, the corresponding active components and related pharmacological mechanism of such medication are not clear yet.					
33964361	2	122	theme	pharmacological	406:420	arg1	mechanism					422:430	related pharmacological mechanism	398:430	related pharmacological mechanism	398:430	However, the corresponding active components and related pharmacological mechanism of such medication are not clear yet.					
33445776	0	0	theme	Rough	71:75	arg1	Purification					97:108	Rough Lipopolysaccharides Purification	71:108	Rough Lipopolysaccharides Purification	71:108	A New Look at the Enterobacterial Common Antigen Forms Obtained during Rough Lipopolysaccharides Purification.					
33445776	5	1	used	used	740:743	arg2	We					737:738	We	737:738	We	737:738	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	2	2	dep	-β-D-ManpNAcA-	268:281	arg1	→3					246:247	→3	246:247	→3	246:247	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	2	2	dep	-β-D-ManpNAcA-	268:281	arg1	1→4					264:266	1→4	264:266	1→4	264:266	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	8	3	theme	tetrameric	1315:1324	arg1	ECACYC					1353:1358	O-acetylated tetrameric, pentameric, and hexameric ECACYC	1302:1358	O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified	1302:1379	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	2	4	dep	lipopolysaccharide	411:428	arg1	ECALPS					443:448	ECALPS	443:448	ECALPS	443:448	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	2	4	dep	lipopolysaccharide	411:428	arg1	endotoxin					432:440	endotoxin	432:440	lipopolysaccharide - endotoxin (ECALPS)	411:449	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	1	5	theme	conserved	153:161	arg1	antigen					163:169	a conserved antigen	151:169	a conserved antigen expressed by enterobacteria	151:197	Enterobacterial common antigen (ECA) is a conserved antigen expressed by enterobacteria.					
33445776	1	5	theme	conserved	153:161	arg1	antigen					134:140	Enterobacterial common antigen	111:140	Enterobacterial common antigen (ECA)	111:146	Enterobacterial common antigen (ECA) is a conserved antigen expressed by enterobacteria.					
33445776	10	6	theme	ECA	1538:1540	arg1	origin					1521:1526	The origin	1517:1526	The origin of linear ECA	1517:1540	The origin of linear ECA is discussed against the current knowledge about ECAPG and ECALPS.					
33445776	7	7	theme	high	1169:1172	arg1	heterogeneity					1174:1186	high heterogeneity	1169:1186	high heterogeneity of the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation	1169:1270	We demonstrated high heterogeneity of the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation.					
33445776	8	8	theme	moiety	1407:1412	arg1	devoid					1391:1396	devoid	1391:1396	devoid	1391:1396	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	8	9	theme	lipid	1401:1405	arg1	moiety					1407:1412	lipid moiety	1401:1412	lipid moiety	1401:1412	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	7	10	gly	heterogeneity	1174:1186	arg1	ECA					1215:1217	the supernatant-derived ECA	1191:1217	the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation	1191:1270	We demonstrated high heterogeneity of the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation.					
33445776	3	11	theme	outer	493:497	arg1	integrity					508:516	outer membrane integrity	493:516	outer membrane integrity	493:516	ECA maintains, outer membrane integrity, immunogenicity, and viability of enterobacteria.					
33445776	0	12	theme	Lipopolysaccharides	77:95	arg1	Purification					97:108	Rough Lipopolysaccharides Purification	71:108	Rough Lipopolysaccharides Purification	71:108	A New Look at the Enterobacterial Common Antigen Forms Obtained during Rough Lipopolysaccharides Purification.					
33445776	0	13	from	Antigen	41:47	arg1	Look					6:9	A New Look	0:9	A New Look at the Enterobacterial Common Antigen	0:47	A New Look at the Enterobacterial Common Antigen Forms Obtained during Rough Lipopolysaccharides Purification.					
33445776	9	14	theme	selected	1499:1506	arg1	strains					1508:1514	all selected strains	1495:1514	all selected strains	1495:1514	Described results were common for all selected strains.					
33445776	8	15	dep	ECACYC	1353:1358	arg1	identified					1370:1379	identified	1370:1379	have been identified	1360:1379	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	5	16	theme	structural	929:938	arg1	analysis					940:947	structural analysis	929:947	structural analysis	929:947	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	17	theme	interaction	814:824	arg1	chromatography					845:858	zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography	789:858	zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography	789:858	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	2	18	theme	cyclic	456:461	arg1	form					463:466	cyclic form	456:466	cyclic form (ECACYC)	456:475	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	2	18	theme	cyclic	456:461	arg1	ECACYC					469:474	ECACYC	469:474	ECACYC	469:474	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	10	19	theme	current	1567:1573	arg1	knowledge					1575:1583	the current knowledge	1563:1583	the current knowledge about ECAPG and ECALPS	1563:1606	The origin of linear ECA is discussed against the current knowledge about ECAPG and ECALPS.					
33445776	6	20	theme	complex	1076:1082	arg1	characteristic					1084:1097	complex characteristic	1076:1097	complex characteristic of all ECA forms present in LPS-derived supernatants	1076:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	1	21	theme	Enterobacterial	111:125	arg1	antigen					163:169	a conserved antigen	151:169	a conserved antigen expressed by enterobacteria	151:197	Enterobacterial common antigen (ECA) is a conserved antigen expressed by enterobacteria.					
33445776	1	21	theme	Enterobacterial	111:125	arg1	ECA					143:145	ECA	143:145	ECA	143:145	Enterobacterial common antigen (ECA) is a conserved antigen expressed by enterobacteria.					
33445776	1	21	theme	Enterobacterial	111:125	arg1	antigen					134:140	Enterobacterial common antigen	111:140	Enterobacterial common antigen (ECA)	111:146	Enterobacterial common antigen (ECA) is a conserved antigen expressed by enterobacteria.					
33445776	8	22	theme	repeating	1444:1452	arg1	units					1454:1458	7 to 11 repeating units	1436:1458	7 to 11 repeating units	1436:1458	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	2	23	theme	linear	348:353	arg1	polysaccharides					355:369	surface-bound linear polysaccharides	334:369	surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC)	334:475	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	0	24	theme	New	2:4	arg1	Look					6:9	A New Look	0:9	A New Look at the Enterobacterial Common Antigen	0:47	A New Look at the Enterobacterial Common Antigen Forms Obtained during Rough Lipopolysaccharides Purification.					
33445776	6	25	theme	present	1116:1122	arg1	forms					1110:1114	all ECA forms	1102:1114	all ECA forms present in LPS-derived supernatants	1102:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	10	26	theme	linear	1531:1536	arg1	ECA					1538:1540	linear ECA	1531:1540	linear ECA	1531:1540	The origin of linear ECA is discussed against the current knowledge about ECAPG and ECALPS.					
33445776	3	27	theme	membrane	499:506	arg1	integrity					508:516	outer membrane integrity	493:516	outer membrane integrity	493:516	ECA maintains, outer membrane integrity, immunogenicity, and viability of enterobacteria.					
33445776	6	28	from	supernatants	1139:1150	arg1	present					1116:1122	present	1116:1122	present	1116:1122	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	2	29	theme	surface-bound	334:346	arg1	polysaccharides					355:369	surface-bound linear polysaccharides	334:369	surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC)	334:475	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	5	30	theme	mild	745:748	arg1	hydrolysis					755:764	mild acid hydrolysis	745:764	mild acid hydrolysis	745:764	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	31	theme	zwitterionic-hydrophilic	789:812	arg1	chromatography					845:858	zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography	789:858	zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography	789:858	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	32	theme	liquid	826:831	arg1	chromatography					845:858	zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography	789:858	zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography	789:858	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	33	theme	acid	750:753	arg1	hydrolysis					755:764	mild acid hydrolysis	745:764	mild acid hydrolysis	745:764	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	6	34	theme	Presented	1030:1038	arg1	report					1058:1063	the first report	1048:1063	the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants	1048:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	6	34	theme	Presented	1030:1038	arg1	work					1040:1043	Presented work	1030:1043	Presented work	1030:1043	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	5	35	theme	R1	995:996	arg1	fractionation					910:922	fractionation	910:922	fractionation	910:922	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	35	theme	R1	995:996	arg1	purification					896:907	purification	896:907	purification	896:907	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	35	theme	R1	995:996	arg1	analysis					940:947	structural analysis	929:947	structural analysis	929:947	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	6	36	theme	ECA	1106:1108	arg1	forms					1110:1114	all ECA forms	1102:1114	all ECA forms present in LPS-derived supernatants	1102:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	6	37	theme	forms	1110:1114	arg1	characteristic					1084:1097	complex characteristic	1076:1097	complex characteristic of all ECA forms present in LPS-derived supernatants	1076:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	6	38	theme	LPS-derived	1127:1137	arg1	supernatants					1139:1150	LPS-derived supernatants	1127:1150	LPS-derived supernatants	1127:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	0	39	dep	Forms	49:53	arg1	Obtained					55:62	Obtained	55:62	Forms Obtained during Rough Lipopolysaccharides Purification	49:108	A New Look at the Enterobacterial Common Antigen Forms Obtained during Rough Lipopolysaccharides Purification.					
33445776	6	40	from	present	1116:1122	arg1	supernatants					1139:1150	LPS-derived supernatants	1127:1150	LPS-derived supernatants	1127:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	5	41	theme	gel	770:772	arg1	filtration					774:783	gel filtration	770:783	gel filtration	770:783	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	0	42	theme	Enterobacterial	18:32	arg1	Antigen					41:47	the Enterobacterial Common Antigen	14:47	the Enterobacterial Common Antigen	14:47	A New Look at the Enterobacterial Common Antigen Forms Obtained during Rough Lipopolysaccharides Purification.					
33445776	8	43	theme	pentameric	1327:1336	arg1	ECACYC					1353:1358	O-acetylated tetrameric, pentameric, and hexameric ECACYC	1302:1358	O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified	1302:1379	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	8	44	theme	devoid	1391:1396	arg1	ECA					1421:1423	devoid of lipid moiety linear ECA	1391:1423	devoid of lipid moiety linear ECA built from 7 to 11 repeating units	1391:1458	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	5	45	dep	Escherichia	978:988	arg1	coli					990:993	coli	990:993	coli	990:993	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	8	46	dep	11	1441:1442	arg1	to					1438:1439	to	1438:1439	to	1438:1439	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	8	47	theme	O-acetylated	1302:1313	arg1	ECACYC					1353:1358	O-acetylated tetrameric, pentameric, and hexameric ECACYC	1302:1358	O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified	1302:1379	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	2	48	attach	linked	371:376	arg1	form					463:466	cyclic form	456:466	cyclic form (ECACYC)	456:475	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	2	48	attach	linked	371:376	arg1	ECAPG					401:405	ECAPG	401:405	ECAPG	401:405	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	2	48	attach	linked	371:376	arg1	ECACYC					469:474	ECACYC	469:474	ECACYC	469:474	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	2	48	attach	linked	371:376	arg2	polysaccharides					355:369	surface-bound linear polysaccharides	334:369	surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC)	334:475	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	2	48	attach	linked	371:376	arg1	lipopolysaccharide					411:428	lipopolysaccharide	411:428	lipopolysaccharide - endotoxin (ECALPS)	411:449	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	2	48	attach	linked	371:376	arg1	phosphoglyceride					383:398	a phosphoglyceride	381:398	a phosphoglyceride (ECAPG)	381:406	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	2	49	theme	repeating	229:237	arg1	units					239:243	trisaccharide repeating units	215:243	trisaccharide repeating units	215:243	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	9	50	theme	Described	1461:1469	arg1	results					1471:1477	Described results	1461:1477	Described results	1461:1477	Described results were common for all selected strains.					
33445776	8	51	theme	hexameric	1343:1351	arg1	ECACYC					1353:1358	O-acetylated tetrameric, pentameric, and hexameric ECACYC	1302:1358	O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified	1302:1379	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	2	52	theme	trisaccharide	215:227	arg1	units					239:243	trisaccharide repeating units	215:243	trisaccharide repeating units	215:243	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	1	53	theme	common	127:132	arg1	antigen					163:169	a conserved antigen	151:169	a conserved antigen expressed by enterobacteria	151:197	Enterobacterial common antigen (ECA) is a conserved antigen expressed by enterobacteria.					
33445776	1	53	theme	common	127:132	arg1	ECA					143:145	ECA	143:145	ECA	143:145	Enterobacterial common antigen (ECA) is a conserved antigen expressed by enterobacteria.					
33445776	1	53	theme	common	127:132	arg1	antigen					134:140	Enterobacterial common antigen	111:140	Enterobacterial common antigen (ECA)	111:146	Enterobacterial common antigen (ECA) is a conserved antigen expressed by enterobacteria.					
33445776	0	54	theme	Common	34:39	arg1	Antigen					41:47	the Enterobacterial Common Antigen	14:47	the Enterobacterial Common Antigen	14:47	A New Look at the Enterobacterial Common Antigen Forms Obtained during Rough Lipopolysaccharides Purification.					
33445776	5	55	theme	K12	1002:1004	arg1	preparations					1016:1027	K12 crude LPS preparations	1002:1027	K12 crude LPS preparations	1002:1027	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	4	56	theme	ECA	650:652	arg1	isolation					654:662	ECA isolation	650:662	ECA isolation	650:662	A supernatant obtained after LPS ultracentrifugation was reported as a source for ECA isolation, but it has never been assessed for detailed composition besides ECACYC.					
33445776	8	57	theme	linear	1414:1419	arg1	ECA					1421:1423	devoid of lipid moiety linear ECA	1391:1423	devoid of lipid moiety linear ECA built from 7 to 11 repeating units	1391:1458	Not only previously reported O-acetylated tetrameric, pentameric, and hexameric ECACYC have been identified, but also devoid of lipid moiety linear ECA built from 7 to 11 repeating units.					
33445776	3	58	theme	enterobacteria	552:565	arg1	immunogenicity					519:532	immunogenicity	519:532	immunogenicity	519:532	ECA maintains, outer membrane integrity, immunogenicity, and viability of enterobacteria.					
33445776	3	58	theme	enterobacteria	552:565	arg1	viability					539:547	viability	539:547	viability	539:547	ECA maintains, outer membrane integrity, immunogenicity, and viability of enterobacteria.					
33445776	3	58	theme	enterobacteria	552:565	arg1	integrity					508:516	outer membrane integrity	493:516	outer membrane integrity	493:516	ECA maintains, outer membrane integrity, immunogenicity, and viability of enterobacteria.					
33445776	2	59	theme	-α-D-Fucp4NAc-	249:262	arg1	-β-D-ManpNAcA-					268:281	→3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→	246:302	→3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→	246:302	It is built by trisaccharide repeating units: →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→ and occurs in three forms: as surface-bound linear polysaccharides linked to a phosphoglyceride (ECAPG) or lipopolysaccharide - endotoxin (ECALPS), and cyclic form (ECACYC).					
33445776	5	60	theme	Escherichia	978:988	arg1	R1					995:996	Escherichia coli R1	978:996	Escherichia coli R1	978:996	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	61	theme	mass	874:877	arg1	spectrometry					879:890	mass spectrometry	874:890	mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations	874:1027	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	7	62	link	supernatant-derived	1195:1213	arg1	ECA					1215:1217	the supernatant-derived ECA	1191:1217	the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation	1191:1270	We demonstrated high heterogeneity of the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation.					
33445776	6	63	link	LPS-derived	1127:1137	arg1	supernatants					1139:1150	LPS-derived supernatants	1127:1150	LPS-derived supernatants	1127:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	4	64	theme	detailed	700:707	arg1	composition					709:719	detailed composition	700:719	detailed composition besides ECACYC	700:734	A supernatant obtained after LPS ultracentrifugation was reported as a source for ECA isolation, but it has never been assessed for detailed composition besides ECACYC.					
33445776	5	65	theme	crude	1006:1010	arg1	preparations					1016:1027	K12 crude LPS preparations	1002:1027	K12 crude LPS preparations	1002:1027	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	66	theme	rough	952:956	arg1	sonnei					967:972	rough Shigella sonnei	952:972	rough Shigella sonnei	952:972	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	6	67	theme	first	1052:1056	arg1	report					1058:1063	the first report	1048:1063	the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants	1048:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	6	67	theme	first	1052:1056	arg1	work					1040:1043	Presented work	1030:1043	Presented work	1030:1043	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	6	68	attach	present	1116:1122	arg1	supernatants					1139:1150	LPS-derived supernatants	1127:1150	LPS-derived supernatants	1127:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	6	68	attach	present	1116:1122	arg2	forms					1110:1114	all ECA forms	1102:1114	all ECA forms present in LPS-derived supernatants	1102:1150	Presented work is the first report concerning complex characteristic of all ECA forms present in LPS-derived supernatants.					
33445776	7	69	theme	supernatant-derived	1195:1213	arg1	ECA					1215:1217	the supernatant-derived ECA	1191:1217	the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation	1191:1270	We demonstrated high heterogeneity of the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation.					
33445776	5	70	theme	LPS	1012:1014	arg1	preparations					1016:1027	K12 crude LPS preparations	1002:1027	K12 crude LPS preparations	1002:1027	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	71	theme	Shigella	958:965	arg1	sonnei					967:972	rough Shigella sonnei	952:972	rough Shigella sonnei	952:972	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	4	72	theme	LPS	597:599	arg1	ultracentrifugation					601:619	LPS ultracentrifugation	597:619	LPS ultracentrifugation	597:619	A supernatant obtained after LPS ultracentrifugation was reported as a source for ECA isolation, but it has never been assessed for detailed composition besides ECACYC.					
33445776	7	73	theme	ECA	1215:1217	arg1	heterogeneity					1174:1186	high heterogeneity	1169:1186	high heterogeneity of the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation	1169:1270	We demonstrated high heterogeneity of the supernatant-derived ECA that contaminate LPS purified by ultracentrifugation.					
33445776	5	74	theme	sonnei	967:972	arg1	fractionation					910:922	fractionation	910:922	fractionation	910:922	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	74	theme	sonnei	967:972	arg1	purification					896:907	purification	896:907	purification	896:907	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	74	theme	sonnei	967:972	arg1	analysis					940:947	structural analysis	929:947	structural analysis	929:947	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
33445776	5	75	theme	ZIC®HILIC	834:842	arg1	chromatography					845:858	zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography	789:858	zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography	789:858	We used mild acid hydrolysis and gel filtration, or zwitterionic-hydrophilic interaction liquid (ZIC®HILIC) chromatography combined with mass spectrometry for purification, fractionation, and structural analysis of rough Shigella sonnei and Escherichia coli R1 and K12 crude LPS preparations.					
34925422	7	0	with	Man-7	1038:1042	arg1	residue					1087:1093	a terminal glucose residue	1068:1093	a terminal glucose residue	1068:1093	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	7	1	contain	contained	989:997	arg2	Man-5					1031:1035	Man-5	1031:1035	Man-5	1031:1035	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	7	1	contain	contained	989:997	arg1	N-glycans					979:987	High mannose type N-glycans	961:987	High mannose type N-glycans	961:987	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	7	1	contain	contained	989:997	arg2	classes					1014:1020	four different classes	999:1020	four different classes	999:1020	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	7	1	contain	contained	989:997	arg2	Man-7					1038:1042	Man-7	1038:1042	Man-7	1038:1042	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	7	1	contain	contained	989:997	arg2	Man-9					1045:1049	Man-9	1045:1049	Man-9	1045:1049	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	7	1	contain	contained	989:997	arg2	Man-10					1056:1061	Man-10	1056:1061	Man-10	1056:1061	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	2	2	used	used	302:305	arg2	approaches					286:295	N-glycoproteomic and N-glycomic approaches	254:295	N-glycoproteomic and N-glycomic approaches	254:295	Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum.					
34925422	10	3	theme	humanized	1573:1581	arg1	N-glycoproteins					1601:1615	the functional humanized biopharmaceutical N-glycoproteins	1558:1615	the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics	1558:1645	The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.					
34925422	2	4	gly	N-glycoproteins	321:335	arg1	N-glycoproteins					321:335	N-glycoproteins	321:335	N-glycoproteins	321:335	Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum.					
34925422	0	5	from	Profiling	7:15	arg1	tricornutum					78:88	the Diatom Phaeodactylum tricornutum	53:88	the Diatom Phaeodactylum tricornutum	53:88	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.					
34925422	9	6	theme	N-glycan	1315:1322	arg1	structures					1324:1333	N-glycan structures	1315:1333	N-glycan structures	1315:1333	The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae.					
34925422	9	7	theme	N-glycoproteomic	1275:1290	arg1	scale					1292:1296	a N-glycoproteomic scale	1273:1296	a N-glycoproteomic scale	1273:1296	The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae.					
34925422	2	8	theme	Phaeodactylum	371:383	arg1	tricornutum					385:395	the model diatom Phaeodactylum tricornutum	354:395	the model diatom Phaeodactylum tricornutum	354:395	Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum.					
34925422	2	9	from	N-glycans	341:349	arg1	tricornutum					385:395	the model diatom Phaeodactylum tricornutum	354:395	the model diatom Phaeodactylum tricornutum	354:395	Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum.					
34925422	10	10	theme	functional	1562:1571	arg1	N-glycoproteins					1601:1615	the functional humanized biopharmaceutical N-glycoproteins	1558:1615	the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics	1558:1645	The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.					
34925422	4	11	theme	important	563:571	arg1	pathways					583:590	important metabolic pathways	563:590	important metabolic pathways in P. tricornutum	563:608	These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum.					
34925422	6	12	theme	high	921:924	arg1	mannose					926:932	high mannose	921:932	high mannose	921:932	Subsequently, 69 N-glycans corresponding to 59 N-glycoproteins were identified and classified into high mannose and hybrid type N-glycans.					
34925422	9	13	from	database	1356:1363	arg1	microalgae					1368:1377	microalgae	1368:1377	microalgae	1368:1377	The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae.					
34925422	3	14	gly	N-glycopeptides	422:436	arg2	N-glycopeptides					422:436	863 different N-glycopeptides	408:436	863 different N-glycopeptides corresponding to 639 N-glycoproteins	408:473	In total, 863 different N-glycopeptides corresponding to 639 N-glycoproteins were identified from P. tricornutum.					
34925422	2	15	dep	N-glycoproteins	321:335	arg1	the					317:319	the	317:319	the	317:319	Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum.					
34925422	7	16	theme	mannose	966:972	arg1	N-glycans					979:987	High mannose type N-glycans	961:987	High mannose type N-glycans	961:987	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	2	17	theme	diatom	364:369	arg1	tricornutum					385:395	the model diatom Phaeodactylum tricornutum	354:395	the model diatom Phaeodactylum tricornutum	354:395	Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum.					
34925422	8	18	theme	terminal	1139:1146	arg1	residue					1155:1161	a terminal GlcNAc residue	1137:1161	a terminal GlcNAc residue	1137:1161	Hybrid type N-glycan harbored Man-4 with a terminal GlcNAc residue.					
34925422	7	19	theme	different	1004:1012	arg1	Man-7					1038:1042	Man-7	1038:1042	Man-7	1038:1042	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	7	19	theme	different	1004:1012	arg1	Man-5					1031:1035	Man-5	1031:1035	Man-5	1031:1035	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	7	19	theme	different	1004:1012	arg1	Man-9					1045:1049	Man-9	1045:1049	Man-9	1045:1049	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	7	19	theme	different	1004:1012	arg1	classes					1014:1020	four different classes	999:1020	four different classes	999:1020	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	7	19	theme	different	1004:1012	arg1	Man-10					1056:1061	Man-10	1056:1061	Man-10	1056:1061	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	10	20	theme	N-glycoproteins	1477:1491	arg1	function					1459:1466	the precise function	1447:1466	the precise function of these N-glycoproteins	1447:1491	The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.					
34925422	7	21	theme	type	974:977	arg1	N-glycans					979:987	High mannose type N-glycans	961:987	High mannose type N-glycans	961:987	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	4	22	from	tricornutum	598:608	arg1	pathways					583:590	important metabolic pathways	563:590	important metabolic pathways in P. tricornutum	563:608	These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum.					
34925422	4	22	from	tricornutum	598:608	arg1	variety					552:558	a variety	550:558	a variety of important metabolic pathways in P. tricornutum	550:608	These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum.					
34925422	1	23	gly	N-glycoproteins	203:217	arg1	N-glycoproteins					203:217	N-glycoproteins	203:217	N-glycoproteins	203:217	N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae.					
34925422	3	24	theme	different	412:420	arg1	N-glycopeptides					422:436	863 different N-glycopeptides	408:436	863 different N-glycopeptides corresponding to 639 N-glycoproteins	408:473	In total, 863 different N-glycopeptides corresponding to 639 N-glycoproteins were identified from P. tricornutum.					
34925422	5	25	gly	N-glycosylation	728:742	arg1	protein					789:795	the protein N-glycosylation pathway	785:819	the protein N-glycosylation pathway	785:819	Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.					
34925422	5	25	gly	N-glycosylation	728:742	arg1	N-glycosylation					797:811	the protein N-glycosylation pathway	785:819	the protein N-glycosylation pathway	785:819	Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.					
34925422	5	25	gly	N-glycosylation	728:742	arg1	proteins					753:760	these proteins	747:760	these proteins	747:760	Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.					
34925422	10	26	gly	N-glycoproteins	1477:1491	arg1	N-glycoproteins					1477:1491	these N-glycoproteins	1471:1491	these N-glycoproteins	1471:1491	The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.					
34925422	7	27	theme	High	961:964	arg1	N-glycans					979:987	High mannose type N-glycans	961:987	High mannose type N-glycans	961:987	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	0	28	theme	Global	0:5	arg1	Profiling					7:15	Global Profiling	0:15	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.	0:89	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.					
34925422	7	29	with	Man-5	1031:1035	arg1	residue					1087:1093	a terminal glucose residue	1068:1093	a terminal glucose residue	1068:1093	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	8	30	theme	GlcNAc	1148:1153	arg1	residue					1155:1161	a terminal GlcNAc residue	1137:1161	a terminal GlcNAc residue	1137:1161	Hybrid type N-glycan harbored Man-4 with a terminal GlcNAc residue.					
34925422	5	31	theme	N-glycosylation	648:662	arg1	pathway					664:670	the N-glycosylation pathway	644:670	the N-glycosylation pathway	644:670	Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.					
34925422	4	32	theme	metabolic	573:581	arg1	pathways					583:590	important metabolic pathways	563:590	important metabolic pathways in P. tricornutum	563:608	These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum.					
34925422	4	33	gly	N-glycoproteins	518:532	arg1	N-glycoproteins					518:532	These N-glycoproteins	512:532	These N-glycoproteins	512:532	These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum.					
34925422	5	34	theme	proteins	753:760	arg1	important					771:779	important	771:779	important	771:779	Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.					
34925422	5	34	theme	proteins	753:760	arg1	N-glycosylation					728:742	the N-glycosylation	724:742	the N-glycosylation of these proteins	724:760	Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.					
34925422	0	35	theme	N-Glycoproteins	20:34	arg1	Profiling					7:15	Global Profiling	0:15	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.	0:89	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.					
34925422	8	36	theme	type	1103:1106	arg1	N-glycan					1108:1115	Hybrid type N-glycan	1096:1115	Hybrid type N-glycan	1096:1115	Hybrid type N-glycan harbored Man-4 with a terminal GlcNAc residue.					
34925422	1	37	from	modification	141:152	arg1	eukaryotes					161:170	all eukaryotes	157:170	all eukaryotes	157:170	N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae.					
34925422	8	38	theme	Hybrid	1096:1101	arg1	N-glycan					1108:1115	Hybrid type N-glycan	1096:1115	Hybrid type N-glycan	1096:1115	Hybrid type N-glycan harbored Man-4 with a terminal GlcNAc residue.					
34925422	9	39	theme	modification	1257:1268	arg1	understanding					1235:1247	our understanding	1231:1247	our understanding of this modification	1231:1268	The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae.					
34925422	6	40	gly	N-glycoproteins	869:883	arg1	N-glycoproteins					869:883	59 N-glycoproteins	866:883	59 N-glycoproteins	866:883	Subsequently, 69 N-glycans corresponding to 59 N-glycoproteins were identified and classified into high mannose and hybrid type N-glycans.					
34925422	5	41	theme	protein	789:795	arg1	pathway					813:819	the protein N-glycosylation pathway	785:819	the protein N-glycosylation pathway	785:819	Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.					
34925422	1	42	theme	important	113:121	arg1	modification					141:152	an important posttranslational modification	110:152	an important posttranslational modification in all eukaryotes	110:170	N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae.					
34925422	1	42	theme	important	113:121	arg1	N-glycosylation					91:105	N-glycosylation	91:105	N-glycosylation	91:105	N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae.					
34925422	9	43	theme	nascent	1205:1211	arg1	proteins					1213:1220	nascent proteins	1205:1220	nascent proteins	1205:1220	The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae.					
34925422	9	44	theme	N-glycosylation	1186:1200	arg1	identification					1168:1181	The identification	1164:1181	The identification of N-glycosylation on nascent proteins	1164:1220	The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae.					
34925422	0	45	theme	N-Glycans	40:48	arg1	Profiling					7:15	Global Profiling	0:15	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.	0:89	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.					
34925422	5	46	theme	N-glycosylation	797:811	arg1	pathway					813:819	the protein N-glycosylation pathway	785:819	the protein N-glycosylation pathway	785:819	Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.					
34925422	10	47	theme	precise	1451:1457	arg1	function					1459:1466	the precise function	1447:1466	the precise function of these N-glycoproteins	1447:1491	The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.					
34925422	3	48	theme	639	455:457	arg1	N-glycoproteins					459:473	639 N-glycoproteins	455:473	639 N-glycoproteins	455:473	In total, 863 different N-glycopeptides corresponding to 639 N-glycoproteins were identified from P. tricornutum.					
34925422	7	49	with	Man-9	1045:1049	arg1	residue					1087:1093	a terminal glucose residue	1068:1093	a terminal glucose residue	1068:1093	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	10	50	theme	clinical	1625:1632	arg1	therapeutics					1634:1645	the clinical therapeutics	1621:1645	the clinical therapeutics	1621:1645	The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.					
34925422	9	51	theme	N-glycan	1347:1354	arg1	database					1356:1363	the N-glycan database	1343:1363	the N-glycan database in microalgae	1343:1377	The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae.					
34925422	5	52	gly	N-glycoproteins	691:705	arg1	proteins					618:625	Twelve proteins	611:625	Twelve proteins participating in the N-glycosylation pathway	611:670	Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.					
34925422	5	52	gly	N-glycoproteins	691:705	arg1	N-glycoproteins					691:705	N-glycoproteins	691:705	N-glycoproteins	691:705	Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway.					
34925422	1	53	from	N-glycans	223:231	arg1	microalgae					236:245	microalgae	236:245	microalgae	236:245	N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae.					
34925422	7	54	with	Man-10	1056:1061	arg1	residue					1087:1093	a terminal glucose residue	1068:1093	a terminal glucose residue	1068:1093	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	4	55	theme	pathways	583:590	arg1	pathways					583:590	important metabolic pathways	563:590	important metabolic pathways in P. tricornutum	563:608	These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum.					
34925422	4	55	theme	pathways	583:590	arg1	variety					552:558	a variety	550:558	a variety of important metabolic pathways in P. tricornutum	550:608	These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum.					
34925422	9	56	theme	structures	1324:1333	arg1	analysis					1303:1310	the analysis	1299:1310	the analysis of N-glycan structures	1299:1333	The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae.					
34925422	1	57	theme	posttranslational	123:139	arg1	modification					141:152	an important posttranslational modification	110:152	an important posttranslational modification in all eukaryotes	110:170	N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae.					
34925422	1	57	theme	posttranslational	123:139	arg1	N-glycosylation					91:105	N-glycosylation	91:105	N-glycosylation	91:105	N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae.					
34925422	4	58	from	pathways	583:590	arg1	tricornutum					598:608	P. tricornutum	595:608	P. tricornutum	595:608	These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum.					
34925422	1	59	from	N-glycoproteins	203:217	arg1	microalgae					236:245	microalgae	236:245	microalgae	236:245	N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae.					
34925422	2	60	theme	model	358:362	arg1	tricornutum					385:395	the model diatom Phaeodactylum tricornutum	354:395	the model diatom Phaeodactylum tricornutum	354:395	Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum.					
34925422	3	61	gly	N-glycoproteins	459:473	arg1	N-glycoproteins					459:473	639 N-glycoproteins	455:473	639 N-glycoproteins	455:473	In total, 863 different N-glycopeptides corresponding to 639 N-glycoproteins were identified from P. tricornutum.					
34925422	6	62	theme	type	945:948	arg1	N-glycans					950:958	hybrid type N-glycans	938:958	hybrid type N-glycans	938:958	Subsequently, 69 N-glycans corresponding to 59 N-glycoproteins were identified and classified into high mannose and hybrid type N-glycans.					
34925422	9	63	from	identification	1168:1181	arg1	proteins					1213:1220	nascent proteins	1205:1220	nascent proteins	1205:1220	The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae.					
34925422	10	64	dep	designing	1523:1531	arg1	future					1516:1521	future	1516:1521	future	1516:1521	The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.					
34925422	6	65	theme	hybrid	938:943	arg1	N-glycans					950:958	hybrid type N-glycans	938:958	hybrid type N-glycans	938:958	Subsequently, 69 N-glycans corresponding to 59 N-glycoproteins were identified and classified into high mannose and hybrid type N-glycans.					
34925422	2	66	theme	N-glycomic	275:284	arg1	approaches					286:295	N-glycoproteomic and N-glycomic approaches	254:295	N-glycoproteomic and N-glycomic approaches	254:295	Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum.					
34925422	7	67	theme	terminal	1070:1077	arg1	residue					1087:1093	a terminal glucose residue	1068:1093	a terminal glucose residue	1068:1093	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	10	68	theme	function	1459:1466	arg1	elucidation					1432:1442	the elucidation	1428:1442	the elucidation of the precise function of these N-glycoproteins	1428:1491	The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.					
34925422	0	69	theme	Phaeodactylum	64:76	arg1	tricornutum					78:88	the Diatom Phaeodactylum tricornutum	53:88	the Diatom Phaeodactylum tricornutum	53:88	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.					
34925422	10	70	theme	biopharmaceutical	1583:1599	arg1	N-glycoproteins					1601:1615	the functional humanized biopharmaceutical N-glycoproteins	1558:1615	the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics	1558:1645	The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.					
34925422	1	71	dep	N-glycoproteins	203:217	arg1	the					199:201	the	199:201	the	199:201	N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae.					
34925422	7	72	theme	glucose	1079:1085	arg1	residue					1087:1093	a terminal glucose residue	1068:1093	a terminal glucose residue	1068:1093	High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue.					
34925422	10	73	gly	N-glycoproteins	1601:1615	arg1	N-glycoproteins					1601:1615	the functional humanized biopharmaceutical N-glycoproteins	1558:1615	the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics	1558:1645	The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.					
34925422	4	74	from	variety	552:558	arg1	tricornutum					598:608	P. tricornutum	595:608	P. tricornutum	595:608	These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum.					
34925422	0	75	theme	Diatom	57:62	arg1	tricornutum					78:88	the Diatom Phaeodactylum tricornutum	53:88	the Diatom Phaeodactylum tricornutum	53:88	Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.					
34925422	2	76	theme	N-glycoproteomic	254:269	arg1	approaches					286:295	N-glycoproteomic and N-glycomic approaches	254:295	N-glycoproteomic and N-glycomic approaches	254:295	Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum.					
34925422	2	77	from	N-glycoproteins	321:335	arg1	tricornutum					385:395	the model diatom Phaeodactylum tricornutum	354:395	the model diatom Phaeodactylum tricornutum	354:395	Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum.					
34483088	8	0	theme	chitosan	1109:1116	arg1	NPs					1118:1120	1% and 5% zinc oxide and chitosan NPs	1084:1120	1% and 5% zinc oxide and chitosan NPs	1084:1120	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	6	1	with	group	928:932	arg1	composite					939:947	composite	939:947	composite containing 10% NPs	939:966	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	0	2	dep	orthodontic	110:120	arg1	adhesive					122:129	adhesive	122:129	adhesive	122:129	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	6	3	theme	mean	849:852	arg1	SBS					854:856	mean SBS	849:856	mean SBS	849:856	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	0	4	theme	bond	67:70	arg1	strength					72:79	the shear bond strength	57:79	the shear bond strength of dental composites	57:100	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	1	5	theme	composites	289:298	arg1	strength					271:278	the shear bond strength	256:278	the shear bond strength (SBS) of composites used for orthodontic bonding	256:327	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	1	5	theme	composites	289:298	arg1	SBS					281:283	SBS	281:283	SBS	281:283	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	6	6	located	found	915:919	arg2	value					905:909	the lowest value	894:909	the lowest value	894:909	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	6	6	located	found	915:919	arg1	group					928:932	the group	924:932	the group with composite containing 10% NPs	924:966	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	0	7	theme	dental	84:89	arg1	composites					91:100	dental composites	84:100	dental composites	84:100	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	0	8	from	Effect	0:5	arg1	strength					72:79	the shear bond strength	57:79	the shear bond strength of dental composites	57:100	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	2	9	contain	containing	374:383	arg1	groups					343:348	METHODS Four groups	330:348	METHODS Four groups	330:348	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	9	contain	containing	374:383	arg2	%					403:403	10%	401:403	10%	401:403	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	9	contain	containing	374:383	arg2	%					390:390	1%	389:390	1%	389:390	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	9	contain	containing	374:383	arg1	composites					353:362	composites	353:362	composites (n = 10)	353:371	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	9	contain	containing	374:383	arg1	n = 10					365:370	n = 10	365:370	n = 10	365:370	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	9	contain	containing	374:383	arg2	%					386:386	0%	385:386	0%	385:386	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	9	contain	containing	374:383	arg2	%					394:394	5%	393:394	5%	393:394	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	6	10	theme	10	960:961	arg1	%					962:962	%	962:962	%	962:962	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	2	11	theme	w/w	405:407	arg1	fillers					412:418	w/w NP fillers	405:418	w/w NP fillers	405:418	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	6	12	theme	highest	832:838	arg1	value					840:844	The highest value	828:844	The highest value of mean SBS	828:856	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	8	13	theme	NPs	1118:1120	arg1	Incorporation					1067:1079	CONCLUSIONS Incorporation	1055:1079	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs	1055:1120	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	1	14	theme	chitosan	240:247	arg1	NPs					249:251	chitosan NPs	240:251	chitosan NPs	240:251	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	8	15	contain	had	1122:1124	arg1	Incorporation					1067:1079	CONCLUSIONS Incorporation	1055:1079	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs	1055:1120	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	8	15	contain	had	1122:1124	arg2	effect					1129:1134	no effect	1126:1134	no effect	1126:1134	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	1	16	theme	orthodontic	309:319	arg1	bonding					321:327	orthodontic bonding	309:327	orthodontic bonding	309:327	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	2	17	theme	bovine	491:496	arg1	incisors					498:505	40 intact bovine incisors	481:505	40 intact bovine incisors	481:505	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	18	theme	incisors	498:505	arg1	surfaces					469:476	the surfaces	465:476	the surfaces of 40 intact bovine incisors	465:505	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	0	19	theme	composites	91:100	arg1	strength					72:79	the shear bond strength	57:79	the shear bond strength of dental composites	57:100	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	2	20	theme	intact	484:489	arg1	incisors					498:505	40 intact bovine incisors	481:505	40 intact bovine incisors	481:505	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	21	used	used	440:443	arg2	n = 10					365:370	n = 10	365:370	n = 10	365:370	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	21	used	used	440:443	arg2	composites					353:362	composites	353:362	composites (n = 10)	353:371	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	21	used	used	440:443	arg2	groups					343:348	METHODS Four groups	330:348	METHODS Four groups	330:348	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	8	22	theme	control	1217:1223	arg1	group					1225:1229	the control group	1213:1229	the control group	1213:1229	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	6	23	theme	lowest	898:903	arg1	value					905:909	the lowest value	894:909	the lowest value	894:909	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	7	24	theme	remnant	982:988	arg1	index					990:994	The adhesive remnant index	969:994	The adhesive remnant index	969:994	The adhesive remnant index did not differ significantly among the groups (P = 0.823).					
34483088	6	25	dep	found	862:866	arg1	RESULTS					820:826	RESULTS	820:826	RESULTS	820:826	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	2	26	dep	%	386:386	arg1	fillers					412:418	w/w NP fillers	405:418	w/w NP fillers	405:418	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	3	27	from	rounds	519:524	arg1	5°C-55°C					548:555	5°C-55°C	548:555	5°C-55°C	548:555	After 1000 rounds of thermal cycling at 5°C-55°C, all specimens were mounted in acrylic blocks.					
34483088	6	28	located	found	862:866	arg1	group					883:887	the control group	871:887	the control group	871:887	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	6	28	located	found	862:866	arg2	value					840:844	The highest value	828:844	The highest value of mean SBS	828:856	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	8	29	theme	CONCLUSIONS	1055:1065	arg1	Incorporation					1067:1079	CONCLUSIONS Incorporation	1055:1079	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs	1055:1120	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	1	30	theme	NPs	249:251	arg1	combination					190:200	the combination	186:200	the combination of zinc oxide nanoparticles (NPs) and chitosan NPs	186:251	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	4	31	theme	index	683:687	arg1	scores					689:694	the adhesive remnant index scores	662:694	the adhesive remnant index scores	662:694	The SBS was tested using a universal testing machine, and the adhesive remnant index scores were registered using a stereomicroscope.					
34483088	2	32	theme	METHODS	330:336	arg1	groups					343:348	METHODS Four groups	330:348	METHODS Four groups	330:348	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	32	theme	METHODS	330:336	arg1	n = 10					365:370	n = 10	365:370	n = 10	365:370	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	32	theme	METHODS	330:336	arg1	composites					353:362	composites	353:362	composites (n = 10)	353:371	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	0	33	theme	oxide	20:24	arg1	Effect					0:5	Effect	0:5	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites	0:100	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	8	34	dep	oxide	1099:1103	arg1	%					1092:1092	%	1092:1092	%	1092:1092	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	1	35	dep	BACKGROUND	132:141	arg1	aimed					154:158	aimed	154:158	aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding	154:327	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	8	36	theme	obtained	1169:1176	arg1	values					1182:1187	the obtained SBS values	1165:1187	the obtained SBS values	1165:1187	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	8	36	theme	obtained	1169:1176	arg1	similar					1194:1200	similar	1194:1200	similar	1194:1200	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	0	37	theme	nano-zinc	10:18	arg1	oxide					20:24	nano-zinc oxide	10:24	nano-zinc oxide	10:24	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	3	38	theme	acrylic	588:594	arg1	blocks					596:601	acrylic blocks	588:601	acrylic blocks	588:601	After 1000 rounds of thermal cycling at 5°C-55°C, all specimens were mounted in acrylic blocks.					
34483088	5	39	theme	Kruskal-Wallis	799:812	arg1	test					814:817	the Kruskal-Wallis test	795:817	the Kruskal-Wallis test	795:817	Data were statistically analyzed using a 1-way ANOVA and the Kruskal-Wallis test.					
34483088	0	40	theme	nano-chitosan	30:42	arg1	particles					44:52	nano-chitosan particles	30:52	nano-chitosan particles	30:52	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	1	41	theme	shear	260:264	arg1	strength					271:278	the shear bond strength	256:278	the shear bond strength (SBS) of composites used for orthodontic bonding	256:327	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	1	41	theme	shear	260:264	arg1	SBS					281:283	SBS	281:283	SBS	281:283	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	6	42	theme	control	875:881	arg1	group					883:887	the control group	871:887	the control group	871:887	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	1	43	theme	combination	190:200	arg1	effect					176:181	the effect	172:181	the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding	172:327	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	3	44	from	5°C-55°C	548:555	arg1	rounds					519:524	1000 rounds	514:524	1000 rounds of thermal cycling at 5°C-55°C	514:555	After 1000 rounds of thermal cycling at 5°C-55°C, all specimens were mounted in acrylic blocks.					
34483088	3	44	from	5°C-55°C	548:555	arg1	cycling					537:543	thermal cycling	529:543	thermal cycling at 5°C-55°C	529:555	After 1000 rounds of thermal cycling at 5°C-55°C, all specimens were mounted in acrylic blocks.					
34483088	1	45	theme	bond	266:269	arg1	strength					271:278	the shear bond strength	256:278	the shear bond strength (SBS) of composites used for orthodontic bonding	256:327	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	1	45	theme	bond	266:269	arg1	SBS					281:283	SBS	281:283	SBS	281:283	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	2	46	theme	composites	353:362	arg1	groups					343:348	METHODS Four groups	330:348	METHODS Four groups	330:348	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	46	theme	composites	353:362	arg1	n = 10					365:370	n = 10	365:370	n = 10	365:370	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	46	theme	composites	353:362	arg1	composites					353:362	composites	353:362	composites (n = 10)	353:371	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	47	theme	NP	409:410	arg1	fillers					412:418	w/w NP fillers	405:418	w/w NP fillers	405:418	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	2	48	theme	bond	448:451	arg1	brackets					453:460	bond brackets	448:460	bond brackets to the surfaces of 40 intact bovine incisors	448:505	METHODS Four groups of composites (n = 10), containing 0%, 1%, 5%, and 10% w/w NP fillers, respectively, were used to bond brackets to the surfaces of 40 intact bovine incisors.					
34483088	6	49	contain	containing	949:958	arg1	composite					939:947	composite	939:947	composite containing 10% NPs	939:966	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	6	49	contain	containing	949:958	arg2	NPs					964:966	10% NPs	960:966	10% NPs	960:966	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	4	50	theme	adhesive	666:673	arg1	scores					689:694	the adhesive remnant index scores	662:694	the adhesive remnant index scores	662:694	The SBS was tested using a universal testing machine, and the adhesive remnant index scores were registered using a stereomicroscope.					
34483088	8	51	theme	oxide	1099:1103	arg1	NPs					1118:1120	1% and 5% zinc oxide and chitosan NPs	1084:1120	1% and 5% zinc oxide and chitosan NPs	1084:1120	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	0	52	theme	particles	44:52	arg1	Effect					0:5	Effect	0:5	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites	0:100	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	4	53	theme	remnant	675:681	arg1	scores					689:694	the adhesive remnant index scores	662:694	the adhesive remnant index scores	662:694	The SBS was tested using a universal testing machine, and the adhesive remnant index scores were registered using a stereomicroscope.					
34483088	3	54	theme	thermal	529:535	arg1	cycling					537:543	thermal cycling	529:543	thermal cycling at 5°C-55°C	529:555	After 1000 rounds of thermal cycling at 5°C-55°C, all specimens were mounted in acrylic blocks.					
34483088	3	55	theme	cycling	537:543	arg1	rounds					519:524	1000 rounds	514:524	1000 rounds of thermal cycling at 5°C-55°C	514:555	After 1000 rounds of thermal cycling at 5°C-55°C, all specimens were mounted in acrylic blocks.					
34483088	0	56	used	used	102:105	arg2	Effect					0:5	Effect	0:5	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites	0:100	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	0	56	used	used	102:105	arg2	strength					72:79	the shear bond strength	57:79	the shear bond strength of dental composites	57:100	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	4	57	theme	universal	631:639	arg1	machine					649:655	a universal testing machine	629:655	a universal testing machine	629:655	The SBS was tested using a universal testing machine, and the adhesive remnant index scores were registered using a stereomicroscope.					
34483088	8	58	theme	zinc	1094:1097	arg1	oxide					1099:1103	1% and 5% zinc oxide	1084:1103	1% and 5% zinc oxide	1084:1103	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	6	59	theme	%	962:962	arg1	NPs					964:966	10% NPs	960:966	10% NPs	960:966	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34483088	1	60	theme	zinc	205:208	arg1	oxide					210:214	zinc oxide	205:214	zinc oxide nanoparticles (NPs)	205:234	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	8	61	dep	%	1092:1092	arg1	5					1091:1091	5	1091:1091	5	1091:1091	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	8	61	dep	%	1092:1092	arg1	%					1085:1085	%	1085:1085	%	1085:1085	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	8	62	theme	SBS	1178:1180	arg1	values					1182:1187	the obtained SBS values	1165:1187	the obtained SBS values	1165:1187	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	8	62	theme	SBS	1178:1180	arg1	similar					1194:1200	similar	1194:1200	similar	1194:1200	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	5	63	theme	1-way	779:783	arg1	ANOVA					785:789	a 1-way ANOVA	777:789	a 1-way ANOVA	777:789	Data were statistically analyzed using a 1-way ANOVA and the Kruskal-Wallis test.					
34483088	1	64	theme	oxide	210:214	arg1	nanoparticles					216:228	zinc oxide nanoparticles	205:228	zinc oxide nanoparticles (NPs)	205:234	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	1	64	theme	oxide	210:214	arg1	NPs					231:233	NPs	231:233	NPs	231:233	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	0	65	theme	shear	61:65	arg1	strength					72:79	the shear bond strength	57:79	the shear bond strength of dental composites	57:100	Effect of nano-zinc oxide and nano-chitosan particles on the shear bond strength of dental composites used as orthodontic adhesive.					
34483088	8	66	theme	composite	1150:1158	arg1	SBS					1143:1145	the SBS	1139:1145	the SBS of composite	1139:1158	CONCLUSIONS Incorporation of 1% and 5% zinc oxide and chitosan NPs had no effect on the SBS of composite, and the obtained SBS values were similar to that of the control group.					
34483088	1	67	from	effect	176:181	arg1	strength					271:278	the shear bond strength	256:278	the shear bond strength (SBS) of composites used for orthodontic bonding	256:327	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	1	67	from	effect	176:181	arg1	SBS					281:283	SBS	281:283	SBS	281:283	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	1	68	theme	nanoparticles	216:228	arg1	combination					190:200	the combination	186:200	the combination of zinc oxide nanoparticles (NPs) and chitosan NPs	186:251	BACKGROUND This study aimed to evaluate the effect of the combination of zinc oxide nanoparticles (NPs) and chitosan NPs on the shear bond strength (SBS) of composites used for orthodontic bonding.					
34483088	4	69	theme	testing	641:647	arg1	machine					649:655	a universal testing machine	629:655	a universal testing machine	629:655	The SBS was tested using a universal testing machine, and the adhesive remnant index scores were registered using a stereomicroscope.					
34483088	7	70	theme	adhesive	973:980	arg1	index					990:994	The adhesive remnant index	969:994	The adhesive remnant index	969:994	The adhesive remnant index did not differ significantly among the groups (P = 0.823).					
34483088	6	71	theme	SBS	854:856	arg1	value					840:844	The highest value	828:844	The highest value of mean SBS	828:856	RESULTS The highest value of mean SBS was found in the control group, and the lowest value was found in the group with composite containing 10% NPs.					
34399474	0	0	theme	ultra-finely	62:73	arg1	properties					30:39	Rheological and water binding properties	0:39	properties	30:39	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	3	1	theme	mineral	625:631	arg1	ions					633:636	mineral ions	625:636	mineral ions	625:636	Solutions were formulated with WBS and with solvents mimicking WBS without phenolic compounds and WBS without phenolics nor mineral ions.					
34399474	3	2	theme	phenolic	576:583	arg1	compounds					585:593	phenolic compounds	576:593	phenolic compounds	576:593	Solutions were formulated with WBS and with solvents mimicking WBS without phenolic compounds and WBS without phenolics nor mineral ions.					
34399474	1	3	theme	phenolic	218:225	arg1	compounds					227:235	phenolic compounds	218:235	phenolic compounds	218:235	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	2	4	theme	oatmeal	457:463	arg1	properties					400:409	the physical properties	387:409	the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution	387:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	5	5	theme	water	901:905	arg1	mobility					907:914	water mobility	901:914	water mobility	901:914	From WBS without mineral ions nor phenolics, the addition of mineral ions led to increased pseudo-plasticity and decreased flow consistency, and decreased water mobility.					
34399474	1	6	theme	compounds	227:235	arg1	amounts					190:196	appreciable amounts	178:196	appreciable amounts of mineral ions and phenolic compounds	178:235	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	6	theme	compounds	227:235	arg1	ions					209:212	mineral ions	201:212	mineral ions	201:212	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	6	theme	compounds	227:235	arg1	compounds					227:235	phenolic compounds	218:235	phenolic compounds	218:235	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	2	7	theme	milled	450:455	arg1	oatmeal					457:463	ultra-finely milled oatmeal	437:463	ultra-finely milled oatmeal	437:463	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	2	8	from	properties	400:409	arg1	solution					491:498	solution	491:498	solution	491:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	5	9	theme	flow	869:872	arg1	consistency					874:884	flow consistency	869:884	flow consistency	869:884	From WBS without mineral ions nor phenolics, the addition of mineral ions led to increased pseudo-plasticity and decreased flow consistency, and decreased water mobility.					
34399474	0	10	theme	birch	99:103	arg1	sap					105:107	white birch sap	93:107	white birch sap	93:107	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	1	11	used	used	248:251	arg2	solvent					266:272	alternate solvent	256:272	alternate solvent for food applications	256:294	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	11	used	used	248:251	arg2	WBS					164:166	WBS	164:166	WBS	164:166	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	11	used	used	248:251	arg2	Sap					159:161	White Birch Sap	147:161	White Birch Sap (WBS)	147:167	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	12	theme	White	147:151	arg1	Sap					159:161	White Birch Sap	147:161	White Birch Sap (WBS)	147:167	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	12	theme	White	147:151	arg1	solvent					266:272	alternate solvent	256:272	alternate solvent for food applications	256:294	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	12	theme	White	147:151	arg1	WBS					164:166	WBS	164:166	WBS	164:166	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	0	13	theme	white	93:97	arg1	sap					105:107	white birch sap	93:107	white birch sap	93:107	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	4	14	theme	water	713:717	arg1	mobility					719:726	water mobility	713:726	water mobility	713:726	The influence of solvent composition was evaluated on flow properties and water mobility of the solutions.					
34399474	6	15	theme	hydrocolloids	1035:1047	arg1	aggregation					1016:1026	phenolic-driven aggregation	1000:1026	phenolic-driven aggregation of the hydrocolloids which also seemed to inhibit guar/xanthan interactions	1000:1102	Addition of phenolic compounds through WBS led to opposite effects possibly due to phenolic-driven aggregation of the hydrocolloids which also seemed to inhibit guar/xanthan interactions.					
34399474	1	16	theme	Birch	153:157	arg1	Sap					159:161	White Birch Sap	147:161	White Birch Sap (WBS)	147:167	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	16	theme	Birch	153:157	arg1	solvent					266:272	alternate solvent	256:272	alternate solvent for food applications	256:294	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	16	theme	Birch	153:157	arg1	WBS					164:166	WBS	164:166	WBS	164:166	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	2	17	theme	gum	422:424	arg1	properties					400:409	the physical properties	387:409	the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution	387:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	0	18	dep	milled	75:80	arg1	Influence					110:118	Influence	110:118	Influence of sap minor constituents	110:144	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	0	19	theme	Rheological	0:10	arg1	properties					30:39	Rheological and water binding properties	0:39	properties	30:39	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	6	20	theme	compounds	938:946	arg1	Addition					917:924	Addition	917:924	Addition of phenolic compounds through WBS	917:958	Addition of phenolic compounds through WBS led to opposite effects possibly due to phenolic-driven aggregation of the hydrocolloids which also seemed to inhibit guar/xanthan interactions.					
34399474	5	21	theme	mineral	807:813	arg1	ions					815:818	mineral ions	807:818	mineral ions	807:818	From WBS without mineral ions nor phenolics, the addition of mineral ions led to increased pseudo-plasticity and decreased flow consistency, and decreased water mobility.					
34399474	0	22	theme	binding	22:28	arg1	properties					30:39	Rheological and water binding properties	0:39	properties	30:39	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	4	23	theme	flow	693:696	arg1	properties					698:707	flow properties	693:707	flow properties	693:707	The influence of solvent composition was evaluated on flow properties and water mobility of the solutions.					
34399474	2	24	theme	WBS	366:368	arg1	composition					351:361	the mineral and phenolic composition	326:361	the mineral and phenolic composition of WBS	326:368	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	0	25	theme	water	16:20	arg1	properties					30:39	Rheological and water binding properties	0:39	properties	30:39	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	2	26	theme	combinations	475:486	arg1	properties					400:409	the physical properties	387:409	the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution	387:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	5	27	theme	increased	827:835	arg1	pseudo-plasticity					837:853	increased pseudo-plasticity	827:853	increased pseudo-plasticity	827:853	From WBS without mineral ions nor phenolics, the addition of mineral ions led to increased pseudo-plasticity and decreased flow consistency, and decreased water mobility.					
34399474	2	28	theme	xanthan	414:420	arg1	gum					422:424	xanthan gum	414:424	xanthan gum	414:424	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	2	29	from	gum	422:424	arg1	solution					491:498	solution	491:498	solution	491:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	4	30	theme	solutions	735:743	arg1	properties					698:707	flow properties	693:707	flow properties	693:707	The influence of solvent composition was evaluated on flow properties and water mobility of the solutions.					
34399474	4	30	theme	solutions	735:743	arg1	mobility					719:726	water mobility	713:726	water mobility	713:726	The influence of solvent composition was evaluated on flow properties and water mobility of the solutions.					
34399474	6	31	theme	phenolic-driven	1000:1014	arg1	aggregation					1016:1026	phenolic-driven aggregation	1000:1026	phenolic-driven aggregation of the hydrocolloids which also seemed to inhibit guar/xanthan interactions	1000:1102	Addition of phenolic compounds through WBS led to opposite effects possibly due to phenolic-driven aggregation of the hydrocolloids which also seemed to inhibit guar/xanthan interactions.					
34399474	2	32	theme	composition	351:361	arg1	effect					316:321	the effect	312:321	the effect of the mineral and phenolic composition of WBS	312:368	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	1	33	theme	alternate	256:264	arg1	Sap					159:161	White Birch Sap	147:161	White Birch Sap (WBS)	147:167	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	33	theme	alternate	256:264	arg1	solvent					266:272	alternate solvent	256:272	alternate solvent for food applications	256:294	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	2	34	from	solution	491:498	arg1	properties					400:409	the physical properties	387:409	the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution	387:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	2	35	from	gum	432:434	arg1	solution					491:498	solution	491:498	solution	491:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	1	36	theme	appreciable	178:188	arg1	amounts					190:196	appreciable amounts	178:196	appreciable amounts of mineral ions and phenolic compounds	178:235	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	36	theme	appreciable	178:188	arg1	ions					209:212	mineral ions	201:212	mineral ions	201:212	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	36	theme	appreciable	178:188	arg1	compounds					227:235	phenolic compounds	218:235	phenolic compounds	218:235	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	2	37	theme	ultra-finely	437:448	arg1	oatmeal					457:463	ultra-finely milled oatmeal	437:463	ultra-finely milled oatmeal	437:463	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	0	38	theme	minor	127:131	arg1	constituents					133:144	sap minor constituents	123:144	sap minor constituents	123:144	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	2	39	theme	guar	427:430	arg1	gum					432:434	guar gum	427:434	guar gum	427:434	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	0	40	theme	xanthan	44:50	arg1	properties					30:39	Rheological and water binding properties	0:39	properties	30:39	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	0	41	theme	sap	123:125	arg1	constituents					133:144	sap minor constituents	123:144	sap minor constituents	123:144	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	2	42	theme	physical	391:398	arg1	properties					400:409	the physical properties	387:409	the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution	387:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	6	43	theme	guar/xanthan	1078:1089	arg1	interactions					1091:1102	guar/xanthan interactions	1078:1102	guar/xanthan interactions	1078:1102	Addition of phenolic compounds through WBS led to opposite effects possibly due to phenolic-driven aggregation of the hydrocolloids which also seemed to inhibit guar/xanthan interactions.					
34399474	1	44	theme	food	278:281	arg1	applications					283:294	food applications	278:294	food applications	278:294	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	45	contain	contains	169:176	arg1	Sap					159:161	White Birch Sap	147:161	White Birch Sap (WBS)	147:167	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	45	contain	contains	169:176	arg1	solvent					266:272	alternate solvent	256:272	alternate solvent for food applications	256:294	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	45	contain	contains	169:176	arg2	compounds					227:235	phenolic compounds	218:235	phenolic compounds	218:235	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	45	contain	contains	169:176	arg1	WBS					164:166	WBS	164:166	WBS	164:166	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	45	contain	contains	169:176	arg2	amounts					190:196	appreciable amounts	178:196	appreciable amounts of mineral ions and phenolic compounds	178:235	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	45	contain	contains	169:176	arg2	ions					209:212	mineral ions	201:212	mineral ions	201:212	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	4	46	theme	composition	664:674	arg1	influence					643:651	The influence	639:651	The influence of solvent composition	639:674	The influence of solvent composition was evaluated on flow properties and water mobility of the solutions.					
34399474	6	47	theme	phenolic	929:936	arg1	compounds					938:946	phenolic compounds	929:946	phenolic compounds	929:946	Addition of phenolic compounds through WBS led to opposite effects possibly due to phenolic-driven aggregation of the hydrocolloids which also seemed to inhibit guar/xanthan interactions.					
34399474	0	48	theme	constituents	133:144	arg1	Influence					110:118	Influence	110:118	Influence of sap minor constituents	110:144	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	2	49	from	oatmeal	457:463	arg1	solution					491:498	solution	491:498	solution	491:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	5	50	theme	mineral	763:769	arg1	ions					771:774	mineral ions	763:774	mineral ions	763:774	From WBS without mineral ions nor phenolics, the addition of mineral ions led to increased pseudo-plasticity and decreased flow consistency, and decreased water mobility.					
34399474	4	51	theme	solvent	656:662	arg1	composition					664:674	solvent composition	656:674	solvent composition	656:674	The influence of solvent composition was evaluated on flow properties and water mobility of the solutions.					
34399474	2	52	theme	gum	432:434	arg1	properties					400:409	the physical properties	387:409	the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution	387:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	2	53	theme	phenolic	342:349	arg1	composition					351:361	the mineral and phenolic composition	326:361	the mineral and phenolic composition of WBS	326:368	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	5	54	theme	ions	815:818	arg1	addition					795:802	the addition	791:802	the addition of mineral ions	791:818	From WBS without mineral ions nor phenolics, the addition of mineral ions led to increased pseudo-plasticity and decreased flow consistency, and decreased water mobility.					
34399474	2	55	theme	mineral	330:336	arg1	composition					351:361	the mineral and phenolic composition	326:361	the mineral and phenolic composition of WBS	326:368	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	1	56	theme	mineral	201:207	arg1	ions					209:212	mineral ions	201:212	mineral ions	201:212	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	0	57	theme	guar	53:56	arg1	properties					30:39	Rheological and water binding properties	0:39	properties	30:39	Rheological and water binding properties of xanthan, guar and ultra-finely milled oatmeal in white birch sap: Influence of sap minor constituents.					
34399474	2	58	from	combinations	475:486	arg1	solution					491:498	solution	491:498	solution	491:498	In this study, the effect of the mineral and phenolic composition of WBS was evaluated on the physical properties of xanthan gum, guar gum, ultra-finely milled oatmeal and their combinations in solution.					
34399474	6	59	theme	opposite	967:974	arg1	effects					976:982	opposite effects	967:982	opposite effects	967:982	Addition of phenolic compounds through WBS led to opposite effects possibly due to phenolic-driven aggregation of the hydrocolloids which also seemed to inhibit guar/xanthan interactions.					
34399474	1	60	theme	ions	209:212	arg1	amounts					190:196	appreciable amounts	178:196	appreciable amounts of mineral ions and phenolic compounds	178:235	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	60	theme	ions	209:212	arg1	ions					209:212	mineral ions	201:212	mineral ions	201:212	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
34399474	1	60	theme	ions	209:212	arg1	compounds					227:235	phenolic compounds	218:235	phenolic compounds	218:235	White Birch Sap (WBS) contains appreciable amounts of mineral ions and phenolic compounds and can be used as alternate solvent for food applications.					
33284290	0	0	theme	electric	106:113	arg1	field					115:119	an electric field	103:119	an electric field	103:119	Homogeneous and efficient production of a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix to promote wound healing.					
33284290	4	1	theme	healing	997:1003	arg1	process					1005:1011	the healing process	993:1011	the healing process	993:1011	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	4	2	theme	BNC	734:736	arg1	composites					738:747	BNC composites	734:747	BNC composites	734:747	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	5	3	theme	%	1133:1133	arg1	viability					1109:1117	bacterial viability	1099:1117	bacterial viability of at least 77%	1099:1133	Compared with BNC and BNC/COL, the LF-impregnated composites mediated a reduction in bacterial viability of at least 77%.					
33284290	6	4	with	Impregnation	1136:1147	arg1	COL					1154:1156	COL	1154:1156	COL	1154:1156	Impregnation with COL significantly improved the cytocompatibility of BNC composites to promote the adhesion and proliferation of fibroblast cells.					
33284290	5	5	theme	bacterial	1099:1107	arg1	viability					1109:1117	bacterial viability	1099:1117	bacterial viability of at least 77%	1099:1133	Compared with BNC and BNC/COL, the LF-impregnated composites mediated a reduction in bacterial viability of at least 77%.					
33284290	1	6	theme	different	237:245	arg1	BNC/LF					268:273	BNC/LF	268:273	BNC/LF	268:273	BNC was functionalized with collagen (COL) and lactoferrin (LF) to form three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology.					
33284290	1	6	theme	different	237:245	arg1	BNC/COL					259:265	BNC/COL	259:265	BNC/COL	259:265	BNC was functionalized with collagen (COL) and lactoferrin (LF) to form three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology.					
33284290	1	6	theme	different	237:245	arg1	composites					247:256	three different composites	231:256	three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology	231:335	BNC was functionalized with collagen (COL) and lactoferrin (LF) to form three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology.					
33284290	1	6	theme	different	237:245	arg1	BNC/LF/COL					279:288	BNC/LF/COL	279:288	BNC/LF/COL	279:288	BNC was functionalized with collagen (COL) and lactoferrin (LF) to form three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology.					
33284290	4	7	dep	capability	679:688	arg1	rate					719:722	transmission rate	706:722	transmission rate	706:722	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	6	8	theme	BNC	1206:1208	arg1	composites					1210:1219	BNC composites	1206:1219	BNC composites	1206:1219	Impregnation with COL significantly improved the cytocompatibility of BNC composites to promote the adhesion and proliferation of fibroblast cells.					
33284290	8	9	theme	practical	1605:1613	arg1	applications					1615:1626	practical applications	1605:1626	practical applications	1605:1626	These results suggest that BNC/LF/COL obtained using the electrophoresis method represents a promising wound dressing for use in practical applications.					
33284290	2	10	theme	processing	534:543	arg1	solution					545:552	processing solution	534:552	processing solution	534:552	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	8	11	theme	electrophoresis	1533:1547	arg1	method					1549:1554	the electrophoresis method	1529:1554	the electrophoresis method	1529:1554	These results suggest that BNC/LF/COL obtained using the electrophoresis method represents a promising wound dressing for use in practical applications.					
33284290	4	12	theme	transmission	706:717	arg1	rate					719:722	transmission rate	706:722	transmission rate	706:722	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	0	13	theme	bacterial	42:50	arg1	composite					87:95	a bacterial nanocellulose-lactoferrin-collagen composite	40:95	a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix	40:131	Homogeneous and efficient production of a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix to promote wound healing.					
33284290	7	14	theme	significant	1445:1455	arg1	reactions					1465:1473	any significant adverse reactions	1441:1473	any significant adverse reactions	1441:1473	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	5	15	from	reduction	1086:1094	arg1	viability					1109:1117	bacterial viability	1099:1117	bacterial viability of at least 77%	1099:1133	Compared with BNC and BNC/COL, the LF-impregnated composites mediated a reduction in bacterial viability of at least 77%.					
33284290	8	16	from	use	1598:1600	arg1	applications					1615:1626	practical applications	1605:1626	practical applications	1605:1626	These results suggest that BNC/LF/COL obtained using the electrophoresis method represents a promising wound dressing for use in practical applications.					
33284290	0	17	theme	Homogeneous	0:10	arg1	production					26:35	Homogeneous and efficient production	0:35	Homogeneous and efficient production of a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix	0:131	Homogeneous and efficient production of a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix to promote wound healing.					
33284290	2	18	theme	immersion-adsorption	393:412	arg1	methods					414:420	traditional immersion-adsorption methods	381:420	traditional immersion-adsorption methods	381:420	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	2	19	theme	protein	470:476	arg1	loading					478:484	greater protein loading	462:484	greater protein loading	462:484	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	2	19	theme	protein	470:476	arg1	homogeneity					494:504	better homogeneity	487:504	better homogeneity	487:504	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	7	20	theme	adverse	1457:1463	arg1	reactions					1465:1473	any significant adverse reactions	1441:1473	any significant adverse reactions	1441:1473	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	6	21	theme	cells	1277:1281	arg1	proliferation					1249:1261	proliferation	1249:1261	proliferation	1249:1261	Impregnation with COL significantly improved the cytocompatibility of BNC composites to promote the adhesion and proliferation of fibroblast cells.					
33284290	6	21	theme	cells	1277:1281	arg1	adhesion					1236:1243	adhesion	1236:1243	adhesion	1236:1243	Impregnation with COL significantly improved the cytocompatibility of BNC composites to promote the adhesion and proliferation of fibroblast cells.					
33284290	2	22	theme	traditional	381:391	arg1	methods					414:420	traditional immersion-adsorption methods	381:420	traditional immersion-adsorption methods	381:420	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	2	23	theme	greater	462:468	arg1	loading					478:484	greater protein loading	462:484	greater protein loading	462:484	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	2	23	theme	greater	462:468	arg1	homogeneity					494:504	better homogeneity	487:504	better homogeneity	487:504	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	8	24	theme	wound	1579:1583	arg1	dressing					1585:1592	a promising wound dressing	1567:1592	a promising wound dressing for use in practical applications	1567:1626	These results suggest that BNC/LF/COL obtained using the electrophoresis method represents a promising wound dressing for use in practical applications.					
33284290	6	25	theme	fibroblast	1266:1275	arg1	cells					1277:1281	fibroblast cells	1266:1281	fibroblast cells	1266:1281	Impregnation with COL significantly improved the cytocompatibility of BNC composites to promote the adhesion and proliferation of fibroblast cells.					
33284290	3	26	theme	great	603:607	arg1	potential					609:617	great potential	603:617	great potential	603:617	Significantly, it has general applicability and great potential for fabricating other similar composites.					
33284290	8	27	theme	promising	1569:1577	arg1	dressing					1585:1592	a promising wound dressing	1567:1592	a promising wound dressing for use in practical applications	1567:1626	These results suggest that BNC/LF/COL obtained using the electrophoresis method represents a promising wound dressing for use in practical applications.					
33284290	3	28	theme	general	577:583	arg1	applicability					585:597	general applicability	577:597	general applicability	577:597	Significantly, it has general applicability and great potential for fabricating other similar composites.					
33284290	2	29	theme	additional	437:446	arg1	advantages					448:457	the additional advantages	433:457	the additional advantages of greater protein loading, better homogeneity	433:504	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	0	30	theme	efficient	16:24	arg1	production					26:35	Homogeneous and efficient production	0:35	Homogeneous and efficient production of a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix	0:131	Homogeneous and efficient production of a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix to promote wound healing.					
33284290	4	31	theme	wound	929:933	arg1	bed					935:937	the wound bed	925:937	the wound bed	925:937	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	4	32	theme	water-holding	665:677	arg1	WVTR					725:728	WVTR	725:728	WVTR	725:728	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	4	32	theme	water-holding	665:677	arg1	capability					679:688	The water-holding capability	661:688	The water-holding capability	661:688	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	7	33	theme	therapeutic	1307:1317	arg1	effect					1319:1324	a greater therapeutic effect	1297:1324	a greater therapeutic effect of BNC/LF/COL	1297:1338	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	4	34	theme	g	838:838	arg1	d-1					844:846	2600 g m-2 d-1	833:846	2600 g m-2 d-1	833:846	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	4	35	theme	water	694:698	arg1	vapor					700:704	water vapor	694:704	water vapor	694:704	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	2	36	theme	better	487:492	arg1	loading					478:484	greater protein loading	462:484	greater protein loading	462:484	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	2	36	theme	better	487:492	arg1	homogeneity					494:504	better homogeneity	487:504	better homogeneity	487:504	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	0	37	theme	wound	144:148	arg1	healing					150:156	wound healing	144:156	wound healing	144:156	Homogeneous and efficient production of a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix to promote wound healing.					
33284290	3	38	theme	other	635:639	arg1	composites					649:658	other similar composites	635:658	other similar composites	635:658	Significantly, it has general applicability and great potential for fabricating other similar composites.					
33284290	4	39	theme	epithelial	960:969	arg1	migration					976:984	epithelial cell migration	960:984	epithelial cell migration	960:984	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	0	40	theme	nanocellulose-lactoferrin-collagen	52:85	arg1	composite					87:95	a bacterial nanocellulose-lactoferrin-collagen composite	40:95	a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix	40:131	Homogeneous and efficient production of a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix to promote wound healing.					
33284290	0	41	theme	composite	87:95	arg1	production					26:35	Homogeneous and efficient production	0:35	Homogeneous and efficient production of a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix	0:131	Homogeneous and efficient production of a bacterial nanocellulose-lactoferrin-collagen composite under an electric field as a matrix to promote wound healing.					
33284290	4	42	theme	d-1	844:846	arg1	WVTR					825:828	a WVTR	823:828	a WVTR of 2600 g m-2 d-1	823:846	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	7	43	theme	greater	1299:1305	arg1	effect					1319:1324	a greater therapeutic effect	1297:1324	a greater therapeutic effect of BNC/LF/COL	1297:1338	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	2	44	theme	loading	478:484	arg1	advantages					448:457	the additional advantages	433:457	the additional advantages of greater protein loading, better homogeneity	433:504	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	2	44	theme	loading	478:484	arg1	requirement					518:528	a lower requirement	510:528	a lower requirement for processing solution	510:552	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	3	45	contain	has	573:575	arg1	it					570:571	it	570:571	it	570:571	Significantly, it has general applicability and great potential for fabricating other similar composites.					
33284290	3	45	contain	has	573:575	arg2	potential					609:617	great potential	603:617	great potential	603:617	Significantly, it has general applicability and great potential for fabricating other similar composites.					
33284290	3	45	contain	has	573:575	arg2	applicability					585:597	general applicability	577:597	general applicability	577:597	Significantly, it has general applicability and great potential for fabricating other similar composites.					
33284290	4	46	theme	moist	902:906	arg1	environment					908:918	a moderately moist environment	889:918	a moderately moist environment	889:918	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	1	47	dep	composites	247:256	arg1	BNC/LF					268:273	BNC/LF	268:273	BNC/LF	268:273	BNC was functionalized with collagen (COL) and lactoferrin (LF) to form three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology.					
33284290	1	47	dep	composites	247:256	arg1	BNC/COL					259:265	BNC/COL	259:265	BNC/COL	259:265	BNC was functionalized with collagen (COL) and lactoferrin (LF) to form three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology.					
33284290	1	47	dep	composites	247:256	arg1	composites					247:256	three different composites	231:256	three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology	231:335	BNC was functionalized with collagen (COL) and lactoferrin (LF) to form three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology.					
33284290	1	47	dep	composites	247:256	arg1	BNC/LF/COL					279:288	BNC/LF/COL	279:288	BNC/LF/COL	279:288	BNC was functionalized with collagen (COL) and lactoferrin (LF) to form three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology.					
33284290	4	48	theme	m-2	840:842	arg1	d-1					844:846	2600 g m-2 d-1	833:846	2600 g m-2 d-1	833:846	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	4	49	theme	composites	738:747	arg1	WVTR					725:728	WVTR	725:728	WVTR	725:728	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	4	49	theme	composites	738:747	arg1	capability					679:688	The water-holding capability	661:688	The water-holding capability	661:688	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	4	49	theme	composites	738:747	arg1	vapor					700:704	water vapor	694:704	water vapor	694:704	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	4	50	theme	cell	971:974	arg1	migration					976:984	epithelial cell migration	960:984	epithelial cell migration	960:984	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	1	51	theme	novel	298:302	arg1	technology					326:335	a novel electrophoresis-based technology	296:335	a novel electrophoresis-based technology	296:335	BNC was functionalized with collagen (COL) and lactoferrin (LF) to form three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology.					
33284290	5	52	theme	LF-impregnated	1049:1062	arg1	composites					1064:1073	the LF-impregnated composites	1045:1073	the LF-impregnated composites	1045:1073	Compared with BNC and BNC/COL, the LF-impregnated composites mediated a reduction in bacterial viability of at least 77%.					
33284290	7	53	theme	wound	1371:1375	arg1	healing					1377:1383	wound healing	1371:1383	wound healing	1371:1383	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	7	54	theme	new	1393:1395	arg1	epithelium					1397:1406	a new epithelium	1391:1406	a new epithelium formed within 9 days	1391:1427	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	4	55	theme	BNC/LF/COL	806:815	arg1	case					798:801	the case	794:801	the case of BNC/LF/COL	794:815	The water-holding capability and water vapor transmission rate (WVTR) of BNC composites were significantly improved, particularly in the case of BNC/LF/COL, with a WVTR of 2600 g m-2 d-1, indicating that the composite maintains a moderately moist environment over the wound bed, which would enhance epithelial cell migration during the healing process.					
33284290	7	56	theme	healing	1377:1383	arg1	model					1362:1366	a rat model	1356:1366	a rat model of wound healing	1356:1383	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	7	57	located	observed	1344:1351	arg2	effect					1319:1324	a greater therapeutic effect	1297:1324	a greater therapeutic effect of BNC/LF/COL	1297:1338	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	7	57	located	observed	1344:1351	arg1	model					1362:1366	a rat model	1356:1366	a rat model of wound healing	1356:1383	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	3	58	theme	similar	641:647	arg1	composites					649:658	other similar composites	635:658	other similar composites	635:658	Significantly, it has general applicability and great potential for fabricating other similar composites.					
33284290	7	59	theme	BNC/LF/COL	1329:1338	arg1	effect					1319:1324	a greater therapeutic effect	1297:1324	a greater therapeutic effect of BNC/LF/COL	1297:1338	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	7	60	theme	rat	1358:1360	arg1	model					1362:1366	a rat model	1356:1366	a rat model of wound healing	1356:1383	Furthermore, a greater therapeutic effect of BNC/LF/COL was observed in a rat model of wound healing, with a new epithelium formed within 9 days and without any significant adverse reactions.					
33284290	2	61	theme	lower	512:516	arg1	requirement					518:528	a lower requirement	510:528	a lower requirement for processing solution	510:552	The technology is less time-consuming than traditional immersion-adsorption methods and offers the additional advantages of greater protein loading, better homogeneity and a lower requirement for processing solution.					
33284290	1	62	theme	electrophoresis-based	304:324	arg1	technology					326:335	a novel electrophoresis-based technology	296:335	a novel electrophoresis-based technology	296:335	BNC was functionalized with collagen (COL) and lactoferrin (LF) to form three different composites: BNC/COL, BNC/LF and BNC/LF/COL using a novel electrophoresis-based technology.					
33284290	6	63	theme	composites	1210:1219	arg1	cytocompatibility					1185:1201	the cytocompatibility	1181:1201	the cytocompatibility of BNC composites to promote the adhesion and proliferation of fibroblast cells	1181:1281	Impregnation with COL significantly improved the cytocompatibility of BNC composites to promote the adhesion and proliferation of fibroblast cells.					
33284290	6	64	dep	adhesion	1236:1243	arg1	the					1232:1234	the	1232:1234	the	1232:1234	Impregnation with COL significantly improved the cytocompatibility of BNC composites to promote the adhesion and proliferation of fibroblast cells.					
34163514	0	0	theme	Nicotiana	110:118	arg1	Plants					132:137	Glycoengineered Nicotiana benthamiana Plants	94:137	Glycoengineered Nicotiana benthamiana Plants	94:137	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.					
34163514	5	1	theme	GCase	847:851	arg1	activity					860:867	GCase enzyme activity	847:867	GCase enzyme activity	847:867	The successfully produced functional GCase exhibited GCase enzyme activity.					
34163514	9	2	theme	mannose	1455:1461	arg1	protein					1481:1487	mannose receptor targeted protein	1455:1487	especially mannose receptor targeted protein	1444:1487	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	9	2	theme	mannose	1455:1461	arg1	proteins					1434:1441	other pharmaceutical proteins	1413:1441	other pharmaceutical proteins	1413:1441	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	8	3	theme	promising	1219:1227	arg1	host					1257:1260	a host	1255:1260	a host for the production of recombinant GCase with a mannosidic-type N-glycan structure	1255:1342	Our research provides a promising alternative plant line as a host for the production of recombinant GCase with a mannosidic-type N-glycan structure.					
34163514	8	3	theme	promising	1219:1227	arg1	alternative					1229:1239	a promising alternative	1217:1239	a promising alternative plant line	1217:1250	Our research provides a promising alternative plant line as a host for the production of recombinant GCase with a mannosidic-type N-glycan structure.					
34163514	0	4	theme	Glycoengineered	94:108	arg1	Plants					132:137	Glycoengineered Nicotiana benthamiana Plants	94:137	Glycoengineered Nicotiana benthamiana Plants	94:137	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.					
34163514	0	5	from	Production	10:19	arg1	Plants					132:137	Glycoengineered Nicotiana benthamiana Plants	94:137	Glycoengineered Nicotiana benthamiana Plants	94:137	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.					
34163514	7	6	theme	β1,2-xylose	1068:1078	arg1	residues					1096:1103	β1,2-xylose and α1,3-fucose residues	1068:1103	β1,2-xylose and α1,3-fucose residues	1068:1103	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	6	theme	β1,2-xylose	1068:1078	arg1	N-glycans					1057:1065	plant-specific N-glycans	1042:1065	plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues)	1042:1104	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	3	7	theme	N-glycan	395:402	arg1	essential					417:425	essential	417:425	essential	417:425	Importantly, the terminal mannose N-glycan structure is essential for the uptake of recombinant GCase into macrophages via the mannose receptor.					
34163514	3	7	theme	N-glycan	395:402	arg1	structure					404:412	the terminal mannose N-glycan structure	374:412	the terminal mannose N-glycan structure	374:412	Importantly, the terminal mannose N-glycan structure is essential for the uptake of recombinant GCase into macrophages via the mannose receptor.					
34163514	9	8	theme	pharmaceutical	1419:1432	arg1	protein					1481:1487	mannose receptor targeted protein	1455:1487	especially mannose receptor targeted protein	1444:1487	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	9	8	theme	pharmaceutical	1419:1432	arg1	proteins					1434:1441	other pharmaceutical proteins	1413:1441	other pharmaceutical proteins	1413:1441	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	9	9	theme	receptor	1463:1470	arg1	protein					1481:1487	mannose receptor targeted protein	1455:1487	especially mannose receptor targeted protein	1444:1487	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	9	9	theme	receptor	1463:1470	arg1	proteins					1434:1441	other pharmaceutical proteins	1413:1441	other pharmaceutical proteins	1413:1441	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	0	10	theme	benthamiana	120:130	arg1	Plants					132:137	Glycoengineered Nicotiana benthamiana Plants	94:137	Glycoengineered Nicotiana benthamiana Plants	94:137	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.					
34163514	9	11	theme	proteins	1434:1441	arg1	production					1399:1408	the production	1395:1408	the production of other pharmaceutical proteins, especially mannose receptor targeted protein,	1395:1488	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	7	12	theme	glycosylation	1129:1141	arg1	sites					1143:1147	all glycosylation sites	1125:1147	all glycosylation sites of purified GCase produced from ΔgntI plants	1125:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	12	theme	glycosylation	1129:1141	arg1	GCase					1161:1165	purified GCase	1152:1165	purified GCase produced from ΔgntI plants	1152:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	13	gly	glycosylation	1129:1141	arg2	sites					1143:1147	all glycosylation sites	1125:1147	all glycosylation sites of purified GCase produced from ΔgntI plants	1125:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	13	gly	glycosylation	1129:1141	arg1	GCase					1161:1165	purified GCase	1152:1165	purified GCase produced from ΔgntI plants	1152:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	13	gly	glycosylation	1129:1141	arg2	GCase					1161:1165	purified GCase	1152:1165	purified GCase produced from ΔgntI plants	1152:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	2	14	theme	replacement	315:325	arg1	therapy					327:333	enzyme replacement therapy	308:333	enzyme replacement therapy	308:333	Recombinant GCase has been used in enzyme replacement therapy to treat Gaucher disease.					
34163514	4	15	theme	N-acetylglucosaminyltransferase	632:662	arg1	GnTI					667:670	GnTI	667:670	GnTI	667:670	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	4	15	theme	N-acetylglucosaminyltransferase	632:662	arg1	I					664:664	N-acetylglucosaminyltransferase I	632:664	N-acetylglucosaminyltransferase I (GnTI)	632:671	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	3	16	theme	terminal	378:385	arg1	essential					417:425	essential	417:425	essential	417:425	Importantly, the terminal mannose N-glycan structure is essential for the uptake of recombinant GCase into macrophages via the mannose receptor.					
34163514	3	16	theme	terminal	378:385	arg1	structure					404:412	the terminal mannose N-glycan structure	374:412	the terminal mannose N-glycan structure	374:412	Importantly, the terminal mannose N-glycan structure is essential for the uptake of recombinant GCase into macrophages via the mannose receptor.					
34163514	6	17	link	mammalian-derived	931:947	arg1	GCase					949:953	the conventional mammalian-derived GCase	914:953	the conventional mammalian-derived GCase	914:953	The enzyme activity was the same as that of the conventional mammalian-derived GCase.					
34163514	4	18	theme	Nicotiana	687:695	arg1	plants					717:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	2	19	theme	enzyme	308:313	arg1	therapy					327:333	enzyme replacement therapy	308:333	enzyme replacement therapy	308:333	Recombinant GCase has been used in enzyme replacement therapy to treat Gaucher disease.					
34163514	4	20	theme	plants	717:722	arg1	latter					729:734	latter	729:734	latter	729:734	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	3	21	theme	mannose	387:393	arg1	essential					417:425	essential	417:425	essential	417:425	Importantly, the terminal mannose N-glycan structure is essential for the uptake of recombinant GCase into macrophages via the mannose receptor.					
34163514	3	21	theme	mannose	387:393	arg1	structure					404:412	the terminal mannose N-glycan structure	374:412	the terminal mannose N-glycan structure	374:412	Importantly, the terminal mannose N-glycan structure is essential for the uptake of recombinant GCase into macrophages via the mannose receptor.					
34163514	4	22	theme	recombinant	524:534	arg1	GCase					536:540	recombinant GCase	524:540	recombinant GCase	524:540	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	8	23	theme	N-glycan	1325:1332	arg1	structure					1334:1342	a mannosidic-type N-glycan structure	1307:1342	a mannosidic-type N-glycan structure	1307:1342	Our research provides a promising alternative plant line as a host for the production of recombinant GCase with a mannosidic-type N-glycan structure.					
34163514	6	24	theme	enzyme	874:879	arg1	activity					881:888	The enzyme activity	870:888	The enzyme activity	870:888	The enzyme activity was the same as that of the conventional mammalian-derived GCase.					
34163514	6	24	theme	enzyme	874:879	arg1	same					898:901	same	898:901	same	898:901	The enzyme activity was the same as that of the conventional mammalian-derived GCase.					
34163514	8	25	theme	recombinant	1284:1294	arg1	GCase					1296:1300	recombinant GCase	1284:1300	recombinant GCase with a mannosidic-type N-glycan structure	1284:1342	Our research provides a promising alternative plant line as a host for the production of recombinant GCase with a mannosidic-type N-glycan structure.					
34163514	8	26	dep	alternative	1229:1239	arg1	line					1247:1250	plant line	1241:1250	a promising alternative plant line	1217:1250	Our research provides a promising alternative plant line as a host for the production of recombinant GCase with a mannosidic-type N-glycan structure.					
34163514	5	27	theme	produced	811:818	arg1	GCase					831:835	The successfully produced functional GCase	794:835	The successfully produced functional GCase	794:835	The successfully produced functional GCase exhibited GCase enzyme activity.					
34163514	8	28	theme	GCase	1296:1300	arg1	production					1270:1279	the production	1266:1279	the production of recombinant GCase with a mannosidic-type N-glycan structure	1266:1342	Our research provides a promising alternative plant line as a host for the production of recombinant GCase with a mannosidic-type N-glycan structure.					
34163514	5	29	theme	enzyme	853:858	arg1	activity					860:867	GCase enzyme activity	847:867	GCase enzyme activity	847:867	The successfully produced functional GCase exhibited GCase enzyme activity.					
34163514	7	30	theme	plant-specific	1042:1055	arg1	residues					1096:1103	β1,2-xylose and α1,3-fucose residues	1068:1103	β1,2-xylose and α1,3-fucose residues	1068:1103	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	30	theme	plant-specific	1042:1055	arg1	N-glycans					1057:1065	plant-specific N-glycans	1042:1065	plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues)	1042:1104	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	2	31	used	used	300:303	arg2	GCase					285:289	Recombinant GCase	273:289	Recombinant GCase	273:289	Recombinant GCase has been used in enzyme replacement therapy to treat Gaucher disease.					
34163514	4	32	theme	ΔgntI	710:714	arg1	plants					717:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	0	33	theme	Transient	0:8	arg1	Production					10:19	Transient Production	0:19	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.	0:138	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.					
34163514	4	34	theme	wild-type	613:621	arg1	plants					717:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	4	35	from	expression	594:603	arg1	plants					717:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	7	36	theme	N-glycan	1015:1022	arg1	structure					1024:1032	a mannosidic-type N-glycan structure	997:1032	a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues)	997:1104	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	36	theme	N-glycan	1015:1022	arg1	predominant					1110:1120	predominant	1110:1120	predominant	1110:1120	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	2	37	theme	Gaucher	344:350	arg1	disease					352:358	Gaucher disease	344:358	Gaucher disease	344:358	Recombinant GCase has been used in enzyme replacement therapy to treat Gaucher disease.					
34163514	7	38	from	predominant	1110:1120	arg1	sites					1143:1147	all glycosylation sites	1125:1147	all glycosylation sites of purified GCase produced from ΔgntI plants	1125:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	38	from	predominant	1110:1120	arg1	GCase					1161:1165	purified GCase	1152:1165	purified GCase produced from ΔgntI plants	1152:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	0	39	theme	Human	24:28	arg1	β-Glucocerebrosidase					30:49	Human β-Glucocerebrosidase	24:49	Human β-Glucocerebrosidase	24:49	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.					
34163514	9	40	theme	targeted	1472:1479	arg1	protein					1481:1487	mannose receptor targeted protein	1455:1487	especially mannose receptor targeted protein	1444:1487	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	9	40	theme	targeted	1472:1479	arg1	proteins					1434:1441	other pharmaceutical proteins	1413:1441	other pharmaceutical proteins	1413:1441	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	7	41	theme	ΔgntI	1181:1185	arg1	plants					1187:1192	ΔgntI plants	1181:1192	ΔgntI plants	1181:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	42	attach	predominant	1110:1120	arg1	sites					1143:1147	all glycosylation sites	1125:1147	all glycosylation sites of purified GCase produced from ΔgntI plants	1125:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	42	attach	predominant	1110:1120	arg1	GCase					1161:1165	purified GCase	1152:1165	purified GCase produced from ΔgntI plants	1152:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	42	attach	predominant	1110:1120	arg2	predominant					1110:1120	predominant	1110:1120	predominant	1110:1120	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	42	attach	predominant	1110:1120	arg2	structure					1024:1032	a mannosidic-type N-glycan structure	997:1032	a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues)	997:1104	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	1	43	theme	functional	225:234	arg1	GCase					265:269	GCase	265:269	GCase	265:269	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	1	43	theme	functional	225:234	arg1	β-glucocerebrosidase					243:262	functional enzyme β-glucocerebrosidase	225:262	functional enzyme β-glucocerebrosidase (GCase)	225:270	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	8	44	with	GCase	1296:1300	arg1	structure					1334:1342	a mannosidic-type N-glycan structure	1307:1342	a mannosidic-type N-glycan structure	1307:1342	Our research provides a promising alternative plant line as a host for the production of recombinant GCase with a mannosidic-type N-glycan structure.					
34163514	9	45	theme	glycoengineered	1350:1364	arg1	plant					1366:1370	This glycoengineered plant	1345:1370	This glycoengineered plant	1345:1370	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	1	46	theme	Gaucher	140:146	arg1	disease					148:154	Gaucher disease	140:154	Gaucher disease	140:154	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	1	46	theme	Gaucher	140:146	arg1	disorder					190:197	an inherited lysosomal storage disorder	159:197	an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase)	159:270	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	1	47	theme	enzyme	236:241	arg1	GCase					265:269	GCase	265:269	GCase	265:269	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	1	47	theme	enzyme	236:241	arg1	β-glucocerebrosidase					243:262	functional enzyme β-glucocerebrosidase	225:262	functional enzyme β-glucocerebrosidase (GCase)	225:270	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	3	48	theme	recombinant	445:455	arg1	GCase					457:461	recombinant GCase	445:461	recombinant GCase	445:461	Importantly, the terminal mannose N-glycan structure is essential for the uptake of recombinant GCase into macrophages via the mannose receptor.					
34163514	3	49	theme	mannose	488:494	arg1	receptor					496:503	the mannose receptor	484:503	the mannose receptor	484:503	Importantly, the terminal mannose N-glycan structure is essential for the uptake of recombinant GCase into macrophages via the mannose receptor.					
34163514	2	50	theme	Recombinant	273:283	arg1	GCase					285:289	Recombinant GCase	273:289	Recombinant GCase	273:289	Recombinant GCase has been used in enzyme replacement therapy to treat Gaucher disease.					
34163514	1	51	theme	β-glucocerebrosidase	243:262	arg1	deficiency					211:220	a deficiency	209:220	a deficiency of functional enzyme β-glucocerebrosidase (GCase)	209:270	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	0	52	theme	β-Glucocerebrosidase	30:49	arg1	Production					10:19	Transient Production	0:19	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.	0:138	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.					
34163514	4	53	theme	transient	584:592	arg1	expression					594:603	Agrobacterium-mediated transient expression	561:603	Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures	561:791	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	3	54	theme	GCase	457:461	arg1	uptake					435:440	the uptake	431:440	the uptake of recombinant GCase into macrophages via the mannose receptor	431:503	Importantly, the terminal mannose N-glycan structure is essential for the uptake of recombinant GCase into macrophages via the mannose receptor.					
34163514	6	55	theme	mammalian-derived	931:947	arg1	GCase					949:953	the conventional mammalian-derived GCase	914:953	the conventional mammalian-derived GCase	914:953	The enzyme activity was the same as that of the conventional mammalian-derived GCase.					
34163514	5	56	theme	functional	820:829	arg1	GCase					831:835	The successfully produced functional GCase	794:835	The successfully produced functional GCase	794:835	The successfully produced functional GCase exhibited GCase enzyme activity.					
34163514	0	57	theme	N-Glycan	72:79	arg1	Structure					81:89	Mannosidic-Type N-Glycan Structure	56:89	Mannosidic-Type N-Glycan Structure	56:89	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.					
34163514	4	58	theme	Agrobacterium-mediated	561:582	arg1	expression					594:603	Agrobacterium-mediated transient expression	561:603	Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures	561:791	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	7	59	theme	purified	1152:1159	arg1	GCase					1161:1165	purified GCase	1152:1165	purified GCase produced from ΔgntI plants	1152:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	6	60	theme	conventional	918:929	arg1	GCase					949:953	the conventional mammalian-derived GCase	914:953	the conventional mammalian-derived GCase	914:953	The enzyme activity was the same as that of the conventional mammalian-derived GCase.					
34163514	4	61	dep	wild-type	613:621	arg1	WT					624:625	WT	624:625	WT	624:625	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	4	62	theme	N-glycan	773:780	arg1	structures					782:791	mannosidic-type N-glycan structures	757:791	mannosidic-type N-glycan structures	757:791	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	0	63	theme	Mannosidic-Type	56:70	arg1	Structure					81:89	Mannosidic-Type N-Glycan Structure	56:89	Mannosidic-Type N-Glycan Structure	56:89	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.					
34163514	7	64	theme	GCase	1161:1165	arg1	sites					1143:1147	all glycosylation sites	1125:1147	all glycosylation sites of purified GCase produced from ΔgntI plants	1125:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	64	theme	GCase	1161:1165	arg1	GCase					1161:1165	purified GCase	1152:1165	purified GCase produced from ΔgntI plants	1152:1192	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	4	65	dep	downregulated	673:685	arg1	GnTI					667:670	GnTI	667:670	GnTI	667:670	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	4	65	dep	downregulated	673:685	arg1	I					664:664	N-acetylglucosaminyltransferase I	632:664	N-acetylglucosaminyltransferase I (GnTI)	632:671	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	0	66	with	Production	10:19	arg1	Structure					81:89	Mannosidic-Type N-Glycan Structure	56:89	Mannosidic-Type N-Glycan Structure	56:89	Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants.					
34163514	4	67	theme	mannosidic-type	757:771	arg1	structures					782:791	mannosidic-type N-glycan structures	757:791	mannosidic-type N-glycan structures	757:791	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	8	68	theme	plant	1241:1245	arg1	line					1247:1250	plant line	1241:1250	a promising alternative plant line	1217:1250	Our research provides a promising alternative plant line as a host for the production of recombinant GCase with a mannosidic-type N-glycan structure.					
34163514	9	69	dep	therapeutic	1494:1504	arg1	uses					1506:1509	uses	1506:1509	uses	1506:1509	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	7	70	theme	mannosidic-type	999:1013	arg1	structure					1024:1032	a mannosidic-type N-glycan structure	997:1032	a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues)	997:1104	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	70	theme	mannosidic-type	999:1013	arg1	predominant					1110:1120	predominant	1110:1120	predominant	1110:1120	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	71	from	sites	1143:1147	arg1	structure					1024:1032	a mannosidic-type N-glycan structure	997:1032	a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues)	997:1104	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	71	from	sites	1143:1147	arg1	predominant					1110:1120	predominant	1110:1120	predominant	1110:1120	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	1	72	theme	inherited	162:170	arg1	disease					148:154	Gaucher disease	140:154	Gaucher disease	140:154	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	1	72	theme	inherited	162:170	arg1	disorder					190:197	an inherited lysosomal storage disorder	159:197	an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase)	159:270	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	8	73	theme	mannosidic-type	1309:1323	arg1	structure					1334:1342	a mannosidic-type N-glycan structure	1307:1342	a mannosidic-type N-glycan structure	1307:1342	Our research provides a promising alternative plant line as a host for the production of recombinant GCase with a mannosidic-type N-glycan structure.					
34163514	4	74	theme	benthamiana	697:707	arg1	plants					717:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	1	75	theme	lysosomal	172:180	arg1	disease					148:154	Gaucher disease	140:154	Gaucher disease	140:154	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	1	75	theme	lysosomal	172:180	arg1	disorder					190:197	an inherited lysosomal storage disorder	159:197	an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase)	159:270	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	4	76	theme	downregulated	673:685	arg1	plants					717:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants	608:722	In this research, recombinant GCase was produced using Agrobacterium-mediated transient expression in both wild-type (WT) and N-acetylglucosaminyltransferase I (GnTI) downregulated Nicotiana benthamiana (ΔgntI) plants, the latter of which accumulates mannosidic-type N-glycan structures.					
34163514	9	77	theme	other	1413:1417	arg1	protein					1481:1487	mannose receptor targeted protein	1455:1487	especially mannose receptor targeted protein	1444:1487	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	9	77	theme	other	1413:1417	arg1	proteins					1434:1441	other pharmaceutical proteins	1413:1441	other pharmaceutical proteins	1413:1441	This glycoengineered plant might be applicable to the production of other pharmaceutical proteins, especially mannose receptor targeted protein, for therapeutic uses.					
34163514	7	78	theme	α1,3-fucose	1084:1094	arg1	residues					1096:1103	β1,2-xylose and α1,3-fucose residues	1068:1103	β1,2-xylose and α1,3-fucose residues	1068:1103	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	7	78	theme	α1,3-fucose	1084:1094	arg1	N-glycans					1057:1065	plant-specific N-glycans	1042:1065	plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues)	1042:1104	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
34163514	1	79	theme	storage	182:188	arg1	disease					148:154	Gaucher disease	140:154	Gaucher disease	140:154	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	1	79	theme	storage	182:188	arg1	disorder					190:197	an inherited lysosomal storage disorder	159:197	an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase)	159:270	Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of functional enzyme β-glucocerebrosidase (GCase).					
34163514	7	80	theme	N-glycan	965:972	arg1	analysis					974:981	N-glycan analysis	965:981	N-glycan analysis	965:981	Notably, N-glycan analysis revealed that a mannosidic-type N-glycan structure lacking plant-specific N-glycans (β1,2-xylose and α1,3-fucose residues) was predominant in all glycosylation sites of purified GCase produced from ΔgntI plants.					
33774232	7	0	theme	chemical	1267:1274	arg1	demand					1286:1291	chemical oxidation demand	1267:1291	chemical oxidation demand (COD)	1267:1297	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	0	theme	chemical	1267:1274	arg1	COD					1294:1296	COD	1294:1296	COD	1294:1296	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	2	1	theme	precipitation	378:390	arg1	method					392:397	a precipitation method	376:397	a precipitation method	376:397	The WO3 was synthesized through a precipitation method, and CQDs were amino-functionalized using ethylenedinitrilotetraacetic acid (EDTA) and ethylenediamine (EDA) through one-pot hydrothermal method.					
33774232	8	2	theme	significant	1377:1387	arg1	activity					1404:1411	a significant photocatalytic activity	1375:1411	a significant photocatalytic activity	1375:1411	Moreover, the WO3/N-CQDs EDA 2.5% shows a significant photocatalytic activity even at higher MB initial concentration with 92.93% removal for 50 ppm MB.					
33774232	6	3	from	1.495 eV	1131:1138	arg1	energy					1103:1108	the bandgap energy	1091:1108	the bandgap energy of WO3 from 2.175 to 1.495 eV	1091:1138	It shows that the combination of N-CQDs and WO3 enhanced visible light absorption, by lowering the bandgap energy of WO3 from 2.175 to 1.495 eV.					
33774232	4	4	theme	produced	694:701	arg1	composites					703:712	The produced composites	690:712	The produced composites	690:712	The produced composites exhibit higher monoclinic WO3 crystallinity, larger surface area, lower bandgap energy and quenched photoluminescence intensity.					
33774232	7	5	theme	performance	1150:1160	arg1	composite					1162:1170	The best performance composite	1141:1170	The best performance composite	1141:1170	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	5	theme	performance	1150:1160	arg1	EDA					1186:1188	WO3/N-CQDs EDA	1175:1188	WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%	1175:1332	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	1	6	theme	tungsten	175:182	arg1	composite					270:278	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	10	7	theme	increasing	1613:1622	arg1	performance					1639:1649	The increasing photocatalytic performance	1609:1649	The increasing photocatalytic performance	1609:1649	The increasing photocatalytic performance is affected by the quenching effect of PL and lower bandgap energy.					
33774232	1	8	theme	sugarcane	211:219	arg1	composite					270:278	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	7	9	dep	EDA	1186:1188	arg1	achieved					1318:1325	achieved	1318:1325	achieved 84.61%	1318:1332	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	11	10	theme	increased	1813:1821	arg1	activity					1838:1845	increased photocatalytic activity	1813:1845	increased photocatalytic activity	1813:1845	The lower intensity of the PL indicates the reduced charge carrier recombination resulting in increased photocatalytic activity.					
33774232	0	11	theme	blue	134:137	arg1	removal					139:145	methylene blue removal	124:145	methylene blue removal	124:145	Synthesis of tungsten oxide/ amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composites for methylene blue removal.					
33774232	3	12	theme	bandgap	606:612	arg1	energy					614:619	the bandgap energy	602:619	the bandgap energy	602:619	It is revealed that N-CQDs incorporation into WO3 alters the bandgap energy, crystallinity, surface area, and photoluminescence (PL) properties.					
33774232	5	13	theme	photocatalytic	911:924	arg1	degradation					926:936	photocatalytic degradation	911:936	photocatalytic degradation	911:936	The as-prepared WO3/N-CQDs composites exhibit better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3.					
33774232	8	14	theme	%	1463:1463	arg1	removal					1465:1471	92.93% removal	1458:1471	92.93% removal	1458:1471	Moreover, the WO3/N-CQDs EDA 2.5% shows a significant photocatalytic activity even at higher MB initial concentration with 92.93% removal for 50 ppm MB.					
33774232	9	15	with	degradation	1576:1586	arg1	removal					1600:1606	86.85% removal	1593:1606	86.85% removal	1593:1606	Subsequently, the composite also has good stability after a sequential 3-times cycle of degradation with 86.85% removal.					
33774232	5	16	theme	better	889:894	arg1	performance					938:948	better adsorption and photocatalytic degradation performance	889:948	better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3	889:993	The as-prepared WO3/N-CQDs composites exhibit better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3.					
33774232	1	17	theme	dots	252:255	arg1	composite					270:278	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	4	18	theme	larger	759:764	arg1	area					774:777	larger surface area	759:777	larger surface area	759:777	The produced composites exhibit higher monoclinic WO3 crystallinity, larger surface area, lower bandgap energy and quenched photoluminescence intensity.					
33774232	8	19	with	concentration	1439:1451	arg1	removal					1465:1471	92.93% removal	1458:1471	92.93% removal	1458:1471	Moreover, the WO3/N-CQDs EDA 2.5% shows a significant photocatalytic activity even at higher MB initial concentration with 92.93% removal for 50 ppm MB.					
33774232	11	20	theme	reduced	1763:1769	arg1	recombination					1786:1798	the reduced charge carrier recombination	1759:1798	the reduced charge carrier recombination resulting in increased photocatalytic activity	1759:1845	The lower intensity of the PL indicates the reduced charge carrier recombination resulting in increased photocatalytic activity.					
33774232	9	21	theme	good	1525:1528	arg1	stability					1530:1538	good stability	1525:1538	good stability	1525:1538	Subsequently, the composite also has good stability after a sequential 3-times cycle of degradation with 86.85% removal.					
33774232	4	22	theme	monoclinic	729:738	arg1	crystallinity					744:756	higher monoclinic WO3 crystallinity	722:756	higher monoclinic WO3 crystallinity	722:756	The produced composites exhibit higher monoclinic WO3 crystallinity, larger surface area, lower bandgap energy and quenched photoluminescence intensity.					
33774232	0	23	theme	quantum	83:89	arg1	WO3/N-CQDs					97:106	WO3/N-CQDs	97:106	WO3/N-CQDs	97:106	Synthesis of tungsten oxide/ amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composites for methylene blue removal.					
33774232	0	23	theme	quantum	83:89	arg1	dots					91:94	quantum dots	83:94	quantum dots (WO3/N-CQDs)	83:107	Synthesis of tungsten oxide/ amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composites for methylene blue removal.					
33774232	5	24	theme	adsorption	896:905	arg1	performance					938:948	better adsorption and photocatalytic degradation performance	889:948	better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3	889:993	The as-prepared WO3/N-CQDs composites exhibit better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3.					
33774232	1	25	theme	present	156:162	arg1	study					164:168	this present study	151:168	this present study	151:168	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	7	26	dep	efficiency	1203:1212	arg1	efficiency					1307:1316	removal efficiency	1299:1316	removal efficiency	1299:1316	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	8	27	theme	WO3/N-CQDs	1349:1358	arg1	%					1367:1367	the WO3/N-CQDs EDA 2.5%	1345:1367	the WO3/N-CQDs EDA 2.5%	1345:1367	Moreover, the WO3/N-CQDs EDA 2.5% shows a significant photocatalytic activity even at higher MB initial concentration with 92.93% removal for 50 ppm MB.					
33774232	7	28	theme	0.02017/min	1250:1260	arg1	constant					1238:1245	constant	1238:1245	constant	1238:1245	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	9	29	theme	sequential	1548:1557	arg1	cycle					1567:1571	a sequential 3-times cycle	1546:1571	a sequential 3-times cycle of degradation with 86.85% removal	1546:1606	Subsequently, the composite also has good stability after a sequential 3-times cycle of degradation with 86.85% removal.					
33774232	6	30	theme	light	1061:1065	arg1	absorption					1067:1076	visible light absorption	1053:1076	visible light absorption	1053:1076	It shows that the combination of N-CQDs and WO3 enhanced visible light absorption, by lowering the bandgap energy of WO3 from 2.175 to 1.495 eV.					
33774232	4	31	theme	quenched	805:812	arg1	intensity					832:840	quenched photoluminescence intensity	805:840	quenched photoluminescence intensity	805:840	The produced composites exhibit higher monoclinic WO3 crystallinity, larger surface area, lower bandgap energy and quenched photoluminescence intensity.					
33774232	4	32	theme	bandgap	786:792	arg1	energy					794:799	lower bandgap energy	780:799	lower bandgap energy	780:799	The produced composites exhibit higher monoclinic WO3 crystallinity, larger surface area, lower bandgap energy and quenched photoluminescence intensity.					
33774232	11	33	theme	carrier	1778:1784	arg1	recombination					1786:1798	the reduced charge carrier recombination	1759:1798	the reduced charge carrier recombination resulting in increased photocatalytic activity	1759:1845	The lower intensity of the PL indicates the reduced charge carrier recombination resulting in increased photocatalytic activity.					
33774232	7	34	theme	constant	1238:1245	arg1	rate					1233:1236	removal rate	1225:1236	removal rate constant of 0.02017/min	1225:1260	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	35	theme	removal	1225:1231	arg1	rate					1233:1236	removal rate	1225:1236	removal rate constant of 0.02017/min	1225:1260	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	9	36	theme	degradation	1576:1586	arg1	cycle					1567:1571	a sequential 3-times cycle	1546:1571	a sequential 3-times cycle of degradation with 86.85% removal	1546:1606	Subsequently, the composite also has good stability after a sequential 3-times cycle of degradation with 86.85% removal.					
33774232	4	37	theme	lower	780:784	arg1	energy					794:799	lower bandgap energy	780:799	lower bandgap energy	780:799	The produced composites exhibit higher monoclinic WO3 crystallinity, larger surface area, lower bandgap energy and quenched photoluminescence intensity.					
33774232	10	38	theme	quenching	1670:1678	arg1	effect					1680:1685	the quenching effect	1666:1685	the quenching effect of PL and lower bandgap energy	1666:1716	The increasing photocatalytic performance is affected by the quenching effect of PL and lower bandgap energy.					
33774232	7	39	theme	%	1222:1222	arg1	demand					1286:1291	chemical oxidation demand	1267:1291	chemical oxidation demand (COD)	1267:1297	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	39	theme	%	1222:1222	arg1	rate					1233:1236	removal rate	1225:1236	removal rate constant of 0.02017/min	1225:1260	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	39	theme	%	1222:1222	arg1	COD					1294:1296	COD	1294:1296	COD	1294:1296	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	39	theme	%	1222:1222	arg1	efficiency					1203:1212	an efficiency	1200:1212	an efficiency of 96.86%	1200:1222	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	12	40	theme	significant	1937:1947	arg1	potential					1949:1957	its significant potential to be utilized for wastewater treatment	1933:1997	its significant potential to be utilized for wastewater treatment	1933:1997	The combination of N-CQDs and WO3 resulted in improved photodegradation, which shows its significant potential to be utilized for wastewater treatment.					
33774232	9	41	theme	86.85	1593:1597	arg1	%					1598:1598	%	1598:1598	%	1598:1598	Subsequently, the composite also has good stability after a sequential 3-times cycle of degradation with 86.85% removal.					
33774232	6	42	dep	1.495 eV	1131:1138	arg1	to					1128:1129	to	1128:1129	to	1128:1129	It shows that the combination of N-CQDs and WO3 enhanced visible light absorption, by lowering the bandgap energy of WO3 from 2.175 to 1.495 eV.					
33774232	10	43	theme	bandgap	1703:1709	arg1	energy					1711:1716	lower bandgap energy	1697:1716	lower bandgap energy	1697:1716	The increasing photocatalytic performance is affected by the quenching effect of PL and lower bandgap energy.					
33774232	0	44	theme	tungsten	13:20	arg1	sugarcane					50:58	tungsten oxide/ amino-functionalized sugarcane	13:58	tungsten oxide/ amino-functionalized sugarcane	13:58	Synthesis of tungsten oxide/ amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composites for methylene blue removal.					
33774232	1	45	theme	simple	321:326	arg1	process					335:341	a simple mixing process	319:341	a simple mixing process	319:341	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	12	46	theme	improved	1894:1901	arg1	photodegradation					1903:1918	improved photodegradation	1894:1918	improved photodegradation	1894:1918	The combination of N-CQDs and WO3 resulted in improved photodegradation, which shows its significant potential to be utilized for wastewater treatment.					
33774232	2	47	theme	one-pot	516:522	arg1	method					537:542	one-pot hydrothermal method	516:542	one-pot hydrothermal method	516:542	The WO3 was synthesized through a precipitation method, and CQDs were amino-functionalized using ethylenedinitrilotetraacetic acid (EDTA) and ethylenediamine (EDA) through one-pot hydrothermal method.					
33774232	11	48	theme	lower	1723:1727	arg1	intensity					1729:1737	The lower intensity	1719:1737	The lower intensity of the PL	1719:1747	The lower intensity of the PL indicates the reduced charge carrier recombination resulting in increased photocatalytic activity.					
33774232	0	49	theme	amino-functionalized	29:48	arg1	sugarcane					50:58	tungsten oxide/ amino-functionalized sugarcane	13:58	tungsten oxide/ amino-functionalized sugarcane	13:58	Synthesis of tungsten oxide/ amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composites for methylene blue removal.					
33774232	6	50	theme	bandgap	1095:1101	arg1	energy					1103:1108	the bandgap energy	1091:1108	the bandgap energy of WO3 from 2.175 to 1.495 eV	1091:1138	It shows that the combination of N-CQDs and WO3 enhanced visible light absorption, by lowering the bandgap energy of WO3 from 2.175 to 1.495 eV.					
33774232	8	51	theme	MB	1428:1429	arg1	concentration					1439:1451	higher MB initial concentration	1421:1451	higher MB initial concentration with 92.93% removal for 50 ppm MB	1421:1485	Moreover, the WO3/N-CQDs EDA 2.5% shows a significant photocatalytic activity even at higher MB initial concentration with 92.93% removal for 50 ppm MB.					
33774232	5	52	theme	WO3/N-CQDs	859:868	arg1	composites					870:879	The as-prepared WO3/N-CQDs composites	843:879	The as-prepared WO3/N-CQDs composites	843:879	The as-prepared WO3/N-CQDs composites exhibit better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3.					
33774232	12	53	theme	N-CQDs	1867:1872	arg1	combination					1852:1862	The combination	1848:1862	The combination of N-CQDs and WO3	1848:1880	The combination of N-CQDs and WO3 resulted in improved photodegradation, which shows its significant potential to be utilized for wastewater treatment.					
33774232	11	54	theme	PL	1746:1747	arg1	intensity					1729:1737	The lower intensity	1719:1737	The lower intensity of the PL	1719:1747	The lower intensity of the PL indicates the reduced charge carrier recombination resulting in increased photocatalytic activity.					
33774232	10	55	theme	PL	1690:1691	arg1	effect					1680:1685	the quenching effect	1666:1685	the quenching effect of PL and lower bandgap energy	1666:1716	The increasing photocatalytic performance is affected by the quenching effect of PL and lower bandgap energy.					
33774232	8	56	theme	92.93	1458:1462	arg1	%					1463:1463	%	1463:1463	%	1463:1463	Moreover, the WO3/N-CQDs EDA 2.5% shows a significant photocatalytic activity even at higher MB initial concentration with 92.93% removal for 50 ppm MB.					
33774232	5	57	theme	methylene	953:961	arg1	performance					938:948	better adsorption and photocatalytic degradation performance	889:948	better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3	889:993	The as-prepared WO3/N-CQDs composites exhibit better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3.					
33774232	0	58	theme	derived-carbon	68:81	arg1	composites					109:118	derived-carbon quantum dots (WO3/N-CQDs) composites	68:118	derived-carbon quantum dots (WO3/N-CQDs) composites	68:118	Synthesis of tungsten oxide/ amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composites for methylene blue removal.					
33774232	7	59	theme	WO3/N-CQDs	1175:1184	arg1	composite					1162:1170	The best performance composite	1141:1170	The best performance composite	1141:1170	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	59	theme	WO3/N-CQDs	1175:1184	arg1	EDA					1186:1188	WO3/N-CQDs EDA	1175:1188	WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%	1175:1332	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	60	theme	oxidation	1276:1284	arg1	demand					1286:1291	chemical oxidation demand	1267:1291	chemical oxidation demand (COD)	1267:1297	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	60	theme	oxidation	1276:1284	arg1	COD					1294:1296	COD	1294:1296	COD	1294:1296	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	61	with	%	1193:1193	arg1	rate					1233:1236	removal rate	1225:1236	removal rate constant of 0.02017/min	1225:1260	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	61	with	%	1193:1193	arg1	demand					1286:1291	chemical oxidation demand	1267:1291	chemical oxidation demand (COD)	1267:1297	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	61	with	%	1193:1193	arg1	COD					1294:1296	COD	1294:1296	COD	1294:1296	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	61	with	%	1193:1193	arg1	efficiency					1203:1212	an efficiency	1200:1212	an efficiency of 96.86%	1200:1222	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	62	theme	best	1145:1148	arg1	composite					1162:1170	The best performance composite	1141:1170	The best performance composite	1141:1170	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	7	62	theme	best	1145:1148	arg1	EDA					1186:1188	WO3/N-CQDs EDA	1175:1188	WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%	1175:1332	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	1	63	theme	oxide/amino-functionalized	184:209	arg1	composite					270:278	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	10	64	theme	photocatalytic	1624:1637	arg1	performance					1639:1649	The increasing photocatalytic performance	1609:1649	The increasing photocatalytic performance	1609:1649	The increasing photocatalytic performance is affected by the quenching effect of PL and lower bandgap energy.					
33774232	5	65	theme	blue	963:966	arg1	methylene					953:961	methylene blue	953:966	methylene blue (MB)	953:971	The as-prepared WO3/N-CQDs composites exhibit better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3.					
33774232	5	65	theme	blue	963:966	arg1	MB					969:970	MB	969:970	MB	969:970	The as-prepared WO3/N-CQDs composites exhibit better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3.					
33774232	6	66	theme	WO3	1040:1042	arg1	combination					1014:1024	the combination	1010:1024	the combination of N-CQDs and WO3	1010:1042	It shows that the combination of N-CQDs and WO3 enhanced visible light absorption, by lowering the bandgap energy of WO3 from 2.175 to 1.495 eV.					
33774232	1	67	theme	bagasse	221:227	arg1	composite					270:278	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	6	68	theme	N-CQDs	1029:1034	arg1	combination					1014:1024	the combination	1010:1024	the combination of N-CQDs and WO3	1010:1042	It shows that the combination of N-CQDs and WO3 enhanced visible light absorption, by lowering the bandgap energy of WO3 from 2.175 to 1.495 eV.					
33774232	11	69	theme	photocatalytic	1823:1836	arg1	activity					1838:1845	increased photocatalytic activity	1813:1845	increased photocatalytic activity	1813:1845	The lower intensity of the PL indicates the reduced charge carrier recombination resulting in increased photocatalytic activity.					
33774232	3	70	theme	surface	637:643	arg1	area					645:648	surface area	637:648	surface area	637:648	It is revealed that N-CQDs incorporation into WO3 alters the bandgap energy, crystallinity, surface area, and photoluminescence (PL) properties.					
33774232	5	71	theme	degradation	926:936	arg1	performance					938:948	better adsorption and photocatalytic degradation performance	889:948	better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3	889:993	The as-prepared WO3/N-CQDs composites exhibit better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3.					
33774232	12	72	theme	wastewater	1978:1987	arg1	treatment					1989:1997	wastewater treatment	1978:1997	wastewater treatment	1978:1997	The combination of N-CQDs and WO3 resulted in improved photodegradation, which shows its significant potential to be utilized for wastewater treatment.					
33774232	5	73	theme	pristine	982:989	arg1	WO3					991:993	the pristine WO3	978:993	the pristine WO3	978:993	The as-prepared WO3/N-CQDs composites exhibit better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3.					
33774232	4	74	theme	surface	766:772	arg1	area					774:777	larger surface area	759:777	larger surface area	759:777	The produced composites exhibit higher monoclinic WO3 crystallinity, larger surface area, lower bandgap energy and quenched photoluminescence intensity.					
33774232	11	75	theme	charge	1771:1776	arg1	recombination					1786:1798	the reduced charge carrier recombination	1759:1798	the reduced charge carrier recombination resulting in increased photocatalytic activity	1759:1845	The lower intensity of the PL indicates the reduced charge carrier recombination resulting in increased photocatalytic activity.					
33774232	4	76	theme	higher	722:727	arg1	crystallinity					744:756	higher monoclinic WO3 crystallinity	722:756	higher monoclinic WO3 crystallinity	722:756	The produced composites exhibit higher monoclinic WO3 crystallinity, larger surface area, lower bandgap energy and quenched photoluminescence intensity.					
33774232	3	77	dep	energy	614:619	arg1	properties					678:687	properties	678:687	properties	678:687	It is revealed that N-CQDs incorporation into WO3 alters the bandgap energy, crystallinity, surface area, and photoluminescence (PL) properties.					
33774232	2	78	theme	hydrothermal	524:535	arg1	method					537:542	one-pot hydrothermal method	516:542	one-pot hydrothermal method	516:542	The WO3 was synthesized through a precipitation method, and CQDs were amino-functionalized using ethylenedinitrilotetraacetic acid (EDTA) and ethylenediamine (EDA) through one-pot hydrothermal method.					
33774232	4	79	theme	WO3	740:742	arg1	crystallinity					744:756	higher monoclinic WO3 crystallinity	722:756	higher monoclinic WO3 crystallinity	722:756	The produced composites exhibit higher monoclinic WO3 crystallinity, larger surface area, lower bandgap energy and quenched photoluminescence intensity.					
33774232	9	80	theme	3-times	1559:1565	arg1	cycle					1567:1571	a sequential 3-times cycle	1546:1571	a sequential 3-times cycle of degradation with 86.85% removal	1546:1606	Subsequently, the composite also has good stability after a sequential 3-times cycle of degradation with 86.85% removal.					
33774232	6	81	theme	visible	1053:1059	arg1	light					1061:1065	visible light	1053:1065	visible light absorption	1053:1076	It shows that the combination of N-CQDs and WO3 enhanced visible light absorption, by lowering the bandgap energy of WO3 from 2.175 to 1.495 eV.					
33774232	8	82	theme	EDA	1360:1362	arg1	%					1367:1367	the WO3/N-CQDs EDA 2.5%	1345:1367	the WO3/N-CQDs EDA 2.5%	1345:1367	Moreover, the WO3/N-CQDs EDA 2.5% shows a significant photocatalytic activity even at higher MB initial concentration with 92.93% removal for 50 ppm MB.					
33774232	1	83	theme	derived-carbon	229:242	arg1	WO3/N-CQDs					258:267	WO3/N-CQDs	258:267	WO3/N-CQDs	258:267	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	1	83	theme	derived-carbon	229:242	arg1	dots					252:255	derived-carbon quantum dots	229:255	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	4	84	theme	photoluminescence	814:830	arg1	intensity					832:840	quenched photoluminescence intensity	805:840	quenched photoluminescence intensity	805:840	The produced composites exhibit higher monoclinic WO3 crystallinity, larger surface area, lower bandgap energy and quenched photoluminescence intensity.					
33774232	0	85	theme	oxide/	22:27	arg1	sugarcane					50:58	tungsten oxide/ amino-functionalized sugarcane	13:58	tungsten oxide/ amino-functionalized sugarcane	13:58	Synthesis of tungsten oxide/ amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composites for methylene blue removal.					
33774232	9	86	theme	%	1598:1598	arg1	removal					1600:1606	86.85% removal	1593:1606	86.85% removal	1593:1606	Subsequently, the composite also has good stability after a sequential 3-times cycle of degradation with 86.85% removal.					
33774232	6	87	theme	WO3	1113:1115	arg1	energy					1103:1108	the bandgap energy	1091:1108	the bandgap energy of WO3 from 2.175 to 1.495 eV	1091:1138	It shows that the combination of N-CQDs and WO3 enhanced visible light absorption, by lowering the bandgap energy of WO3 from 2.175 to 1.495 eV.					
33774232	9	88	contain	has	1521:1523	arg1	composite					1506:1514	the composite	1502:1514	the composite	1502:1514	Subsequently, the composite also has good stability after a sequential 3-times cycle of degradation with 86.85% removal.					
33774232	9	88	contain	has	1521:1523	arg2	stability					1530:1538	good stability	1525:1538	good stability	1525:1538	Subsequently, the composite also has good stability after a sequential 3-times cycle of degradation with 86.85% removal.					
33774232	8	89	theme	photocatalytic	1389:1402	arg1	activity					1404:1411	a significant photocatalytic activity	1375:1411	a significant photocatalytic activity	1375:1411	Moreover, the WO3/N-CQDs EDA 2.5% shows a significant photocatalytic activity even at higher MB initial concentration with 92.93% removal for 50 ppm MB.					
33774232	10	90	theme	lower	1697:1701	arg1	energy					1711:1716	lower bandgap energy	1697:1716	lower bandgap energy	1697:1716	The increasing photocatalytic performance is affected by the quenching effect of PL and lower bandgap energy.					
33774232	0	91	theme	sugarcane	50:58	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of tungsten oxide/ amino-functionalized sugarcane	0:58	Synthesis of tungsten oxide/ amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composites for methylene blue removal.					
33774232	1	92	theme	mixing	328:333	arg1	process					335:341	a simple mixing process	319:341	a simple mixing process	319:341	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	1	93	theme	quantum	244:250	arg1	WO3/N-CQDs					258:267	WO3/N-CQDs	258:267	WO3/N-CQDs	258:267	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	1	93	theme	quantum	244:250	arg1	dots					252:255	derived-carbon quantum dots	229:255	the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite	171:278	In this present study, the tungsten oxide/amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composite has successfully been prepared through a simple mixing process.					
33774232	0	94	dep	derived-carbon	68:81	arg1	WO3/N-CQDs					97:106	WO3/N-CQDs	97:106	WO3/N-CQDs	97:106	Synthesis of tungsten oxide/ amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composites for methylene blue removal.					
33774232	0	94	dep	derived-carbon	68:81	arg1	dots					91:94	quantum dots	83:94	quantum dots (WO3/N-CQDs)	83:107	Synthesis of tungsten oxide/ amino-functionalized sugarcane bagasse derived-carbon quantum dots (WO3/N-CQDs) composites for methylene blue removal.					
33774232	2	95	theme	ethylenedinitrilotetraacetic	441:468	arg1	acid					470:473	ethylenedinitrilotetraacetic acid	441:473	ethylenedinitrilotetraacetic acid (EDTA)	441:480	The WO3 was synthesized through a precipitation method, and CQDs were amino-functionalized using ethylenedinitrilotetraacetic acid (EDTA) and ethylenediamine (EDA) through one-pot hydrothermal method.					
33774232	2	95	theme	ethylenedinitrilotetraacetic	441:468	arg1	EDTA					476:479	EDTA	476:479	EDTA	476:479	The WO3 was synthesized through a precipitation method, and CQDs were amino-functionalized using ethylenedinitrilotetraacetic acid (EDTA) and ethylenediamine (EDA) through one-pot hydrothermal method.					
33774232	5	96	theme	as-prepared	847:857	arg1	composites					870:879	The as-prepared WO3/N-CQDs composites	843:879	The as-prepared WO3/N-CQDs composites	843:879	The as-prepared WO3/N-CQDs composites exhibit better adsorption and photocatalytic degradation performance of methylene blue (MB) than the pristine WO3.					
33774232	12	97	theme	WO3	1878:1880	arg1	combination					1852:1862	The combination	1848:1862	The combination of N-CQDs and WO3	1848:1880	The combination of N-CQDs and WO3 resulted in improved photodegradation, which shows its significant potential to be utilized for wastewater treatment.					
33774232	7	98	theme	removal	1299:1305	arg1	efficiency					1307:1316	removal efficiency	1299:1316	removal efficiency	1299:1316	The best performance composite is WO3/N-CQDs EDA 2.5% with an efficiency of 96.86%, removal rate constant of 0.02017/min, and chemical oxidation demand (COD) removal efficiency achieved 84.61%.					
33774232	8	99	theme	higher	1421:1426	arg1	concentration					1439:1451	higher MB initial concentration	1421:1451	higher MB initial concentration with 92.93% removal for 50 ppm MB	1421:1485	Moreover, the WO3/N-CQDs EDA 2.5% shows a significant photocatalytic activity even at higher MB initial concentration with 92.93% removal for 50 ppm MB.					
33774232	8	100	theme	initial	1431:1437	arg1	concentration					1439:1451	higher MB initial concentration	1421:1451	higher MB initial concentration with 92.93% removal for 50 ppm MB	1421:1485	Moreover, the WO3/N-CQDs EDA 2.5% shows a significant photocatalytic activity even at higher MB initial concentration with 92.93% removal for 50 ppm MB.					
33774232	10	101	theme	energy	1711:1716	arg1	effect					1680:1685	the quenching effect	1666:1685	the quenching effect of PL and lower bandgap energy	1666:1716	The increasing photocatalytic performance is affected by the quenching effect of PL and lower bandgap energy.					
33774232	3	102	theme	N-CQDs	565:570	arg1	incorporation					572:584	N-CQDs incorporation	565:584	N-CQDs incorporation into WO3	565:593	It is revealed that N-CQDs incorporation into WO3 alters the bandgap energy, crystallinity, surface area, and photoluminescence (PL) properties.					
33268175	3	0	theme	chemical	369:376	arg1	properties					378:387	Microbiological, physicochemical and chemical properties	332:387	Microbiological, physicochemical and chemical properties	332:387	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	4	1	theme	FTIR	562:565	arg1	analysis					567:574	FTIR analysis	562:574	FTIR analysis of polysaccharide extracts	562:601	FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins.					
33268175	5	2	theme	L.	846:847	arg1	extract					865:871	L. edodes kombucha extract	846:871	L. edodes kombucha extract	846:871	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	5	3	theme	higher	770:775	arg1	content					777:783	a higher content	768:783	a higher content of total polysaccharides, phenols and flavonoids	768:832	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	1	4	theme	Coriolus	183:190	arg1	versicolor					192:201	Coriolus versicolor	183:201	Coriolus versicolor	183:201	Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals.					
33268175	5	5	theme	edodes	849:854	arg1	extract					865:871	L. edodes kombucha extract	846:871	L. edodes kombucha extract	846:871	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	4	6	theme	polysaccharide	579:592	arg1	extracts					594:601	polysaccharide extracts	579:601	polysaccharide extracts	579:601	FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins.					
33268175	5	7	theme	more	734:737	arg1	polysaccharides					747:761	more complex polysaccharides	734:761	more complex polysaccharides	734:761	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	8	8	theme	immunomodulatory	1244:1259	arg1	effects					1261:1267	potentially beneficial immunomodulatory effects	1221:1267	potentially beneficial immunomodulatory effects in allergies	1221:1280	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	5	9	theme	complex	739:745	arg1	polysaccharides					747:761	more complex polysaccharides	734:761	more complex polysaccharides	734:761	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	8	10	with	nutraceuticals	1201:1214	arg1	effects					1261:1267	potentially beneficial immunomodulatory effects	1221:1267	potentially beneficial immunomodulatory effects in allergies	1221:1280	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	0	11	theme	immunomodulatory	101:116	arg1	potential					118:126	immunomodulatory potential	101:126	immunomodulatory potential of their polysaccharide extracts	101:159	Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts.					
33268175	6	12	dep	500 µg/ml	930:938	arg1	up					924:925	up	924:925	up	924:925	The extracts were not cytotoxic for PBMC in vitro up to 500 µg/ml, while immunomodulatory effects depended on their chemical compositions.					
33268175	8	13	theme	kombucha	1135:1142	arg1	beverages					1188:1196	functional beverages	1177:1196	functional beverages	1177:1196	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	8	13	theme	kombucha	1135:1142	arg1	products					1144:1151	novel kombucha products	1129:1151	novel kombucha products	1129:1151	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	8	13	theme	kombucha	1135:1142	arg1	nutraceuticals					1201:1214	nutraceuticals	1201:1214	nutraceuticals with potentially beneficial immunomodulatory effects in allergies	1201:1280	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	5	14	theme	C.	693:694	arg1	extract					716:722	C. versicolor kombucha extract	693:722	C. versicolor kombucha extract	693:722	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	2	15	theme	kombucha	313:320	arg1	beverage					322:329	novel kombucha beverage	307:329	novel kombucha beverage	307:329	Here we used fruiting bodies to prepare novel kombucha beverage.					
33268175	7	16	from	reduction	1050:1058	arg1	cultures					1095:1102	PBMC cultures	1090:1102	PBMC cultures	1090:1102	The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures.					
33268175	5	17	theme	flavonoids	823:832	arg1	content					777:783	a higher content	768:783	a higher content of total polysaccharides, phenols and flavonoids	768:832	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	6	18	theme	immunomodulatory	947:962	arg1	effects					964:970	immunomodulatory effects	947:970	immunomodulatory effects	947:970	The extracts were not cytotoxic for PBMC in vitro up to 500 µg/ml, while immunomodulatory effects depended on their chemical compositions.					
33268175	0	19	theme	versicolor	47:56	arg1	characteristics					13:27	Fermentation characteristics	0:27	Fermentation characteristics of novel Coriolus versicolor and Lentinus	0:69	Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts.					
33268175	2	20	theme	novel	307:311	arg1	beverage					322:329	novel kombucha beverage	307:329	novel kombucha beverage	307:329	Here we used fruiting bodies to prepare novel kombucha beverage.					
33268175	1	21	theme	Lentinus	207:214	arg1	edodes					216:221	Lentinus edodes	207:221	Lentinus edodes	207:221	Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals.					
33268175	3	22	theme	immunological	431:443	arg1	properties					445:454	the immunological properties	427:454	the immunological properties of kombucha polysaccharide extracts	427:490	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	5	23	theme	kombucha	707:714	arg1	extract					716:722	C. versicolor kombucha extract	693:722	C. versicolor kombucha extract	693:722	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	7	24	theme	prominent	1022:1030	arg1	effect					1032:1037	The most prominent effect	1013:1037	The most prominent effect	1013:1037	The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures.					
33268175	0	25	theme	Fermentation	0:11	arg1	characteristics					13:27	Fermentation characteristics	0:27	Fermentation characteristics of novel Coriolus versicolor and Lentinus	0:69	Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts.					
33268175	4	26	theme	polysaccharides	631:645	arg1	presence					619:626	dominant presence	610:626	dominant presence of polysaccharides	610:645	FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins.					
33268175	0	27	theme	novel	32:36	arg1	versicolor					47:56	novel Coriolus versicolor	32:56	novel Coriolus versicolor	32:56	Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts.					
33268175	0	28	theme	extracts	152:159	arg1	beverages					87:95	kombucha beverages	78:95	kombucha beverages	78:95	Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts.					
33268175	0	28	theme	extracts	152:159	arg1	potential					118:126	immunomodulatory potential	101:126	immunomodulatory potential of their polysaccharide extracts	101:159	Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts.					
33268175	3	29	theme	cell	540:543	arg1	cultures					552:559	peripheral blood mononuclear cell (PBMC) cultures	511:559	peripheral blood mononuclear cell (PBMC) cultures	511:559	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	0	30	theme	polysaccharide	137:150	arg1	extracts					152:159	their polysaccharide extracts	131:159	their polysaccharide extracts	131:159	Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts.					
33268175	6	31	theme	chemical	990:997	arg1	compositions					999:1010	their chemical compositions	984:1010	their chemical compositions	984:1010	The extracts were not cytotoxic for PBMC in vitro up to 500 µg/ml, while immunomodulatory effects depended on their chemical compositions.					
33268175	3	32	theme	polysaccharide	468:481	arg1	extracts					483:490	kombucha polysaccharide extracts	459:490	kombucha polysaccharide extracts	459:490	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	5	33	theme	total	788:792	arg1	polysaccharides					794:808	total polysaccharides	788:808	total polysaccharides	788:808	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	5	34	theme	polysaccharides	794:808	arg1	content					777:783	a higher content	768:783	a higher content of total polysaccharides, phenols and flavonoids	768:832	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	2	35	used	used	275:278	arg2	we					272:273	we	272:273	we	272:273	Here we used fruiting bodies to prepare novel kombucha beverage.					
33268175	3	36	theme	blood	522:526	arg1	cultures					552:559	peripheral blood mononuclear cell (PBMC) cultures	511:559	peripheral blood mononuclear cell (PBMC) cultures	511:559	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	0	37	theme	Coriolus	38:45	arg1	versicolor					47:56	novel Coriolus versicolor	32:56	novel Coriolus versicolor	32:56	Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts.					
33268175	3	38	theme	extracts	483:490	arg1	properties					445:454	the immunological properties	427:454	the immunological properties of kombucha polysaccharide extracts	427:490	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	3	39	theme	mononuclear	528:538	arg1	cultures					552:559	peripheral blood mononuclear cell (PBMC) cultures	511:559	peripheral blood mononuclear cell (PBMC) cultures	511:559	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	0	40	theme	Lentinus	62:69	arg1	characteristics					13:27	Fermentation characteristics	0:27	Fermentation characteristics of novel Coriolus versicolor and Lentinus	0:69	Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts.					
33268175	4	41	theme	extracts	594:601	arg1	analysis					567:574	FTIR analysis	562:574	FTIR analysis of polysaccharide extracts	562:601	FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins.					
33268175	3	42	theme	physicochemical	349:363	arg1	properties					378:387	Microbiological, physicochemical and chemical properties	332:387	Microbiological, physicochemical and chemical properties	332:387	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	7	43	theme	PBMC	1090:1093	arg1	cultures					1095:1102	PBMC cultures	1090:1102	PBMC cultures	1090:1102	The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures.					
33268175	3	44	theme	kombucha	459:466	arg1	extracts					483:490	kombucha polysaccharide extracts	459:490	kombucha polysaccharide extracts	459:490	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	5	45	theme	versicolor	696:705	arg1	extract					716:722	C. versicolor kombucha extract	693:722	C. versicolor kombucha extract	693:722	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	8	46	from	effects	1261:1267	arg1	allergies					1272:1280	allergies	1272:1280	allergies	1272:1280	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	8	47	with	beverages	1188:1196	arg1	effects					1261:1267	potentially beneficial immunomodulatory effects	1221:1267	potentially beneficial immunomodulatory effects in allergies	1221:1280	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	8	48	theme	novel	1129:1133	arg1	beverages					1188:1196	functional beverages	1177:1196	functional beverages	1177:1196	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	8	48	theme	novel	1129:1133	arg1	products					1144:1151	novel kombucha products	1129:1151	novel kombucha products	1129:1151	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	8	48	theme	novel	1129:1133	arg1	nutraceuticals					1201:1214	nutraceuticals	1201:1214	nutraceuticals with potentially beneficial immunomodulatory effects in allergies	1201:1280	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	4	49	theme	dominant	610:617	arg1	presence					619:626	dominant presence	610:626	dominant presence of polysaccharides	610:645	FTIR analysis of polysaccharide extracts showed dominant presence of polysaccharides, in addition to phenols, lipids and proteins.					
33268175	3	50	theme	Microbiological	332:346	arg1	properties					378:387	Microbiological, physicochemical and chemical properties	332:387	Microbiological, physicochemical and chemical properties	332:387	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	8	51	theme	beneficial	1233:1242	arg1	effects					1261:1267	potentially beneficial immunomodulatory effects	1221:1267	potentially beneficial immunomodulatory effects in allergies	1221:1280	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	7	52	theme	IL-10	1081:1085	arg1	reduction					1050:1058	the reduction	1046:1058	the reduction of Th2 cytokines and IL-10 in PBMC cultures	1046:1102	The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures.					
33268175	3	53	theme	PBMC	546:549	arg1	cultures					552:559	peripheral blood mononuclear cell (PBMC) cultures	511:559	peripheral blood mononuclear cell (PBMC) cultures	511:559	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	5	54	theme	kombucha	856:863	arg1	extract					865:871	L. edodes kombucha extract	846:871	L. edodes kombucha extract	846:871	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
33268175	7	55	theme	Th2	1063:1065	arg1	cytokines					1067:1075	Th2 cytokines	1063:1075	Th2 cytokines	1063:1075	The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures.					
33268175	1	56	theme	Medicinal	162:170	arg1	mushrooms					172:180	Medicinal mushrooms	162:180	Medicinal mushrooms	162:180	Medicinal mushrooms, Coriolus versicolor and Lentinus edodes are extremely attractive as nutraceuticals.					
33268175	0	57	theme	kombucha	78:85	arg1	beverages					87:95	kombucha beverages	78:95	kombucha beverages	78:95	Fermentation characteristics of novel Coriolus versicolor and Lentinus edodes kombucha beverages and immunomodulatory potential of their polysaccharide extracts.					
33268175	7	58	theme	cytokines	1067:1075	arg1	reduction					1050:1058	the reduction	1046:1058	the reduction of Th2 cytokines and IL-10 in PBMC cultures	1046:1102	The most prominent effect was on the reduction of Th2 cytokines and IL-10 in PBMC cultures.					
33268175	3	59	theme	peripheral	511:520	arg1	cultures					552:559	peripheral blood mononuclear cell (PBMC) cultures	511:559	peripheral blood mononuclear cell (PBMC) cultures	511:559	Microbiological, physicochemical and chemical properties were monitored for eleven days, while the immunological properties of kombucha polysaccharide extracts were determined in peripheral blood mononuclear cell (PBMC) cultures.					
33268175	2	60	theme	fruiting	280:287	arg1	bodies					289:294	fruiting bodies	280:294	fruiting bodies	280:294	Here we used fruiting bodies to prepare novel kombucha beverage.					
33268175	8	61	theme	functional	1177:1186	arg1	products					1144:1151	novel kombucha products	1129:1151	novel kombucha products	1129:1151	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	8	61	theme	functional	1177:1186	arg1	beverages					1188:1196	functional beverages	1177:1196	functional beverages	1177:1196	Based on these results, novel kombucha products could be recommended as functional beverages or nutraceuticals with potentially beneficial immunomodulatory effects in allergies.					
33268175	5	62	theme	phenols	811:817	arg1	content					777:783	a higher content	768:783	a higher content of total polysaccharides, phenols and flavonoids	768:832	C. versicolor kombucha extract displayed more complex polysaccharides, and a higher content of total polysaccharides, phenols and flavonoids compared to L. edodes kombucha extract.					
34263899	4	0	theme	exquisite	678:686	arg1	reactivity					696:705	the exquisite surface reactivity	674:705	the exquisite surface reactivity of the amyloid fibrils	674:728	The formed hybrid materials show enhanced resistance upon compression, without any loss of the exquisite surface reactivity of the amyloid fibrils.					
34263899	2	1	theme	wider	347:351	arg1	basin					359:363	a wider usage basin	345:363	a wider usage basin	345:363	While the mechanical properties of pure amyloid fibril gels meet the needs of some applications, mechanical fragility often hinders a wider usage basin.					
34263899	1	2	from	bio-colloids	101:112	arg1	fields					139:144	many fields	134:144	many fields (e.g. water purification, cell growth, drug delivery and sensing)	134:210	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	1	3	theme	cell	172:175	arg1	growth					177:182	cell growth	172:182	cell growth	172:182	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	1	4	theme	amyloid	81:87	arg1	bio-colloids					101:112	bio-colloids	101:112	bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing)	101:210	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	1	4	theme	amyloid	81:87	arg1	fibrils					89:95	β-Lactoglobulin amyloid fibrils	65:95	β-Lactoglobulin amyloid fibrils	65:95	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	3	5	theme	Gellan	534:539	arg1	polysaccharides					502:516	polysaccharides	502:516	polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh	502:580	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	3	5	theme	Gellan	534:539	arg1	Gum					541:543	low-acetylated Gellan Gum	519:543	low-acetylated Gellan Gum	519:543	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	3	6	dep	polysaccharides	502:516	arg1	polysaccharides					502:516	polysaccharides	502:516	polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh	502:580	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	3	6	dep	polysaccharides	502:516	arg1	κ-carrageenan					549:561	κ-carrageenan	549:561	κ-carrageenan	549:561	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	3	6	dep	polysaccharides	502:516	arg1	Gum					541:543	low-acetylated Gellan Gum	519:543	low-acetylated Gellan Gum	519:543	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	5	7	theme	composite	784:792	arg1	materials					794:802	composite materials	784:802	composite materials that are both highly functional and environmentally friendly	784:863	The proposed approach can pave the way for designing composite materials that are both highly functional and environmentally friendly.					
34263899	3	8	theme	polysaccharides	502:516	arg1	diffusion					489:497	the diffusion	485:497	the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh	485:580	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	3	9	theme	simple	393:398	arg1	approach					416:423	a simple and sustainable approach	391:423	a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels	391:477	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	3	10	theme	sustainable	404:414	arg1	approach					416:423	a simple and sustainable approach	391:423	a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels	391:477	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	2	11	theme	gels	268:271	arg1	properties					234:243	the mechanical properties	219:243	the mechanical properties of pure amyloid fibril gels	219:271	While the mechanical properties of pure amyloid fibril gels meet the needs of some applications, mechanical fragility often hinders a wider usage basin.					
34263899	0	12	theme	amyloid	26:32	arg1	hydrogels					41:49	Polysaccharide-reinforced amyloid fibril hydrogels	0:49	Polysaccharide-reinforced amyloid fibril hydrogels	0:49	Polysaccharide-reinforced amyloid fibril hydrogels and aerogels.					
34263899	2	13	dep	the	278:280	arg1	needs					282:286	needs	282:286	needs	282:286	While the mechanical properties of pure amyloid fibril gels meet the needs of some applications, mechanical fragility often hinders a wider usage basin.					
34263899	4	14	theme	fibrils	722:728	arg1	reactivity					696:705	the exquisite surface reactivity	674:705	the exquisite surface reactivity of the amyloid fibrils	674:728	The formed hybrid materials show enhanced resistance upon compression, without any loss of the exquisite surface reactivity of the amyloid fibrils.					
34263899	1	15	theme	drug	185:188	arg1	delivery					190:197	drug delivery	185:197	drug delivery	185:197	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	0	16	theme	Polysaccharide-reinforced	0:24	arg1	hydrogels					41:49	Polysaccharide-reinforced amyloid fibril hydrogels	0:49	Polysaccharide-reinforced amyloid fibril hydrogels	0:49	Polysaccharide-reinforced amyloid fibril hydrogels and aerogels.					
34263899	1	17	theme	high	117:120	arg1	interest					122:129	high interest	117:129	high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing)	117:210	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	4	18	theme	amyloid	714:720	arg1	fibrils					722:728	the amyloid fibrils	710:728	the amyloid fibrils	710:728	The formed hybrid materials show enhanced resistance upon compression, without any loss of the exquisite surface reactivity of the amyloid fibrils.					
34263899	1	19	from	fields	139:144	arg1	bio-colloids					101:112	bio-colloids	101:112	bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing)	101:210	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	1	19	from	fields	139:144	arg1	fibrils					89:95	β-Lactoglobulin amyloid fibrils	65:95	β-Lactoglobulin amyloid fibrils	65:95	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	1	20	theme	interest	122:129	arg1	bio-colloids					101:112	bio-colloids	101:112	bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing)	101:210	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	1	20	theme	interest	122:129	arg1	fibrils					89:95	β-Lactoglobulin amyloid fibrils	65:95	β-Lactoglobulin amyloid fibrils	65:95	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	3	21	theme	amyloid	441:447	arg1	hydrogels					456:464	amyloid fibril hydrogels	441:464	amyloid fibril hydrogels	441:464	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	0	22	theme	fibril	34:39	arg1	hydrogels					41:49	Polysaccharide-reinforced amyloid fibril hydrogels	0:49	Polysaccharide-reinforced amyloid fibril hydrogels	0:49	Polysaccharide-reinforced amyloid fibril hydrogels and aerogels.					
34263899	1	23	dep	purification	158:169	arg1	e.g.					147:150	e.g.	147:150	e.g.	147:150	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	3	24	theme	fibril	449:454	arg1	hydrogels					456:464	amyloid fibril hydrogels	441:464	amyloid fibril hydrogels	441:464	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	3	25	attach	present	383:389	arg2	we					380:381	we	380:381	we	380:381	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	3	25	attach	present	383:389	arg1	work					374:377	this work	369:377	this work	369:377	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	2	26	theme	mechanical	223:232	arg1	properties					234:243	the mechanical properties	219:243	the mechanical properties of pure amyloid fibril gels	219:271	While the mechanical properties of pure amyloid fibril gels meet the needs of some applications, mechanical fragility often hinders a wider usage basin.					
34263899	1	27	theme	many	134:137	arg1	fields					139:144	many fields	134:144	many fields (e.g. water purification, cell growth, drug delivery and sensing)	134:210	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	4	28	theme	surface	688:694	arg1	reactivity					696:705	the exquisite surface reactivity	674:705	the exquisite surface reactivity of the amyloid fibrils	674:728	The formed hybrid materials show enhanced resistance upon compression, without any loss of the exquisite surface reactivity of the amyloid fibrils.					
34263899	1	29	from	interest	122:129	arg1	fields					139:144	many fields	134:144	many fields (e.g. water purification, cell growth, drug delivery and sensing)	134:210	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	4	30	theme	reactivity	696:705	arg1	loss					666:669	any loss	662:669	any loss of the exquisite surface reactivity of the amyloid fibrils	662:728	The formed hybrid materials show enhanced resistance upon compression, without any loss of the exquisite surface reactivity of the amyloid fibrils.					
34263899	2	31	theme	fibril	261:266	arg1	gels					268:271	pure amyloid fibril gels	248:271	pure amyloid fibril gels	248:271	While the mechanical properties of pure amyloid fibril gels meet the needs of some applications, mechanical fragility often hinders a wider usage basin.					
34263899	4	32	theme	formed	587:592	arg1	materials					601:609	The formed hybrid materials	583:609	The formed hybrid materials	583:609	The formed hybrid materials show enhanced resistance upon compression, without any loss of the exquisite surface reactivity of the amyloid fibrils.					
34263899	2	33	theme	amyloid	253:259	arg1	gels					268:271	pure amyloid fibril gels	248:271	pure amyloid fibril gels	248:271	While the mechanical properties of pure amyloid fibril gels meet the needs of some applications, mechanical fragility often hinders a wider usage basin.					
34263899	2	34	theme	mechanical	310:319	arg1	fragility					321:329	mechanical fragility	310:329	mechanical fragility	310:329	While the mechanical properties of pure amyloid fibril gels meet the needs of some applications, mechanical fragility often hinders a wider usage basin.					
34263899	5	35	theme	proposed	735:742	arg1	approach					744:751	The proposed approach	731:751	The proposed approach	731:751	The proposed approach can pave the way for designing composite materials that are both highly functional and environmentally friendly.					
34263899	2	36	theme	pure	248:251	arg1	gels					268:271	pure amyloid fibril gels	248:271	pure amyloid fibril gels	248:271	While the mechanical properties of pure amyloid fibril gels meet the needs of some applications, mechanical fragility often hinders a wider usage basin.					
34263899	4	37	theme	enhanced	616:623	arg1	resistance					625:634	enhanced resistance	616:634	enhanced resistance	616:634	The formed hybrid materials show enhanced resistance upon compression, without any loss of the exquisite surface reactivity of the amyloid fibrils.					
34263899	1	38	theme	water	152:156	arg1	purification					158:169	water purification	152:169	water purification	152:169	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	3	39	theme	low-acetylated	519:532	arg1	polysaccharides					502:516	polysaccharides	502:516	polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh	502:580	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	3	39	theme	low-acetylated	519:532	arg1	Gum					541:543	low-acetylated Gellan Gum	519:543	low-acetylated Gellan Gum	519:543	In this work, we present a simple and sustainable approach for reinforcing amyloid fibril hydrogels and aerogels, upon the diffusion of polysaccharides (low-acetylated Gellan Gum and κ-carrageenan) inside their mesh.					
34263899	1	40	theme	β-Lactoglobulin	65:79	arg1	bio-colloids					101:112	bio-colloids	101:112	bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing)	101:210	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	1	40	theme	β-Lactoglobulin	65:79	arg1	fibrils					89:95	β-Lactoglobulin amyloid fibrils	65:95	β-Lactoglobulin amyloid fibrils	65:95	β-Lactoglobulin amyloid fibrils are bio-colloids of high interest in many fields (e.g. water purification, cell growth, drug delivery and sensing).					
34263899	2	41	theme	usage	353:357	arg1	basin					359:363	a wider usage basin	345:363	a wider usage basin	345:363	While the mechanical properties of pure amyloid fibril gels meet the needs of some applications, mechanical fragility often hinders a wider usage basin.					
34263899	4	42	theme	hybrid	594:599	arg1	materials					601:609	The formed hybrid materials	583:609	The formed hybrid materials	583:609	The formed hybrid materials show enhanced resistance upon compression, without any loss of the exquisite surface reactivity of the amyloid fibrils.					
34699821	6	0	theme	pseudo-rod	987:996	arg1	particles					1004:1012	pseudo-rod shape particles	987:1012	pseudo-rod shape particles	987:1012	Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo-spherical, while CSeNPs also contained pseudo-rod shape particles.					
34699821	1	1	from	research	183:190	arg1	reuse					199:203	its reuse	195:203	its reuse	195:203	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	1	2	dep	treatment	100:108	arg1	The					96:98	The	96:98	The	96:98	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	10	3	dep	formation	1551:1559	arg1	the					1547:1549	the	1547:1549	the	1547:1549	The present study demonstrated that proteins, polysaccharides, humic-like and fulvic acid-like substances cooperated in the formation and stabilization of SeNPs.					
34699821	9	4	theme	polysaccharides	1356:1370	arg1	different					1405:1413	different	1405:1413	different	1405:1413	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	4	theme	polysaccharides	1356:1370	arg1	contents					1277:1284	the relative contents	1264:1284	the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces	1264:1388	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	4	theme	polysaccharides	1356:1370	arg1	P < 0.05					1416:1423	P < 0.05	1416:1423	P < 0.05	1416:1423	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	5	5	theme	morphology	742:751	arg1	size					753:756	The synthesis process, zeta potential and morphology size	700:756	The synthesis process, zeta potential and morphology size of SeNPs	700:765	The synthesis process, zeta potential and morphology size of SeNPs were changed by pH value, extract concentration and extract composition.					
34699821	12	6	theme	light	1716:1720	arg1	condition					1722:1730	the light condition	1712:1730	the light condition	1712:1730	Under the light condition, CSeNPs could degrade Rhodamine B faster without adding hydrogen peroxide.					
34699821	11	7	dep	CSeNPs	1602:1607	arg1	bandgap					1610:1616	bandgap	1610:1616	bandgap: 1.68 eV	1610:1625	Furthermore, CSeNPs (bandgap: 1.68 eV) had more desirable photocatalytic performance than DSeNPs (bandgap: 1.84 eV).					
34699821	9	8	theme	SeNPs	1239:1243	arg1	images					1221:1226	the fluorescence images	1204:1226	the fluorescence images of the two SeNPs	1204:1243	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	6	9	contain	contained	977:985	arg1	CSeNPs					965:970	CSeNPs	965:970	CSeNPs	965:970	Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo-spherical, while CSeNPs also contained pseudo-rod shape particles.					
34699821	6	9	contain	contained	977:985	arg2	particles					1004:1012	pseudo-rod shape particles	987:1012	pseudo-rod shape particles	987:1012	Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo-spherical, while CSeNPs also contained pseudo-rod shape particles.					
34699821	13	10	theme	resource	1855:1862	arg1	utilization					1864:1874	the resource utilization	1851:1874	the resource utilization of activated sludge	1851:1894	This experiment provided a new insight into the resource utilization of activated sludge and a reference for the synthesis of nanometer selenium with excellent performance.					
34699821	11	11	dep	bandgap	1687:1693	arg1	1.84 eV					1696:1702	1.84 eV	1696:1702	bandgap: 1.84 eV	1687:1702	Furthermore, CSeNPs (bandgap: 1.68 eV) had more desirable photocatalytic performance than DSeNPs (bandgap: 1.84 eV).					
34699821	5	12	theme	synthesis	704:712	arg1	process					714:720	synthesis process	704:720	synthesis process	704:720	The synthesis process, zeta potential and morphology size of SeNPs were changed by pH value, extract concentration and extract composition.					
34699821	9	13	theme	relative	1268:1275	arg1	different					1405:1413	different	1405:1413	different	1405:1413	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	13	theme	relative	1268:1275	arg1	contents					1277:1284	the relative contents	1264:1284	the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces	1264:1388	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	13	theme	relative	1268:1275	arg1	P < 0.05					1416:1423	P < 0.05	1416:1423	P < 0.05	1416:1423	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	14	theme	proteins	1289:1296	arg1	different					1405:1413	different	1405:1413	different	1405:1413	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	14	theme	proteins	1289:1296	arg1	contents					1277:1284	the relative contents	1264:1284	the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces	1264:1388	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	14	theme	proteins	1289:1296	arg1	P < 0.05					1416:1423	P < 0.05	1416:1423	P < 0.05	1416:1423	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	11	15	contain	had	1628:1630	arg1	CSeNPs					1602:1607	CSeNPs	1602:1607	CSeNPs (bandgap: 1.68 eV)	1602:1626	Furthermore, CSeNPs (bandgap: 1.68 eV) had more desirable photocatalytic performance than DSeNPs (bandgap: 1.84 eV).					
34699821	11	15	contain	had	1628:1630	arg2	performance					1662:1672	more desirable photocatalytic performance	1632:1672	more desirable photocatalytic performance	1632:1672	Furthermore, CSeNPs (bandgap: 1.68 eV) had more desirable photocatalytic performance than DSeNPs (bandgap: 1.84 eV).					
34699821	5	16	theme	process	714:720	arg1	size					753:756	The synthesis process, zeta potential and morphology size	700:756	The synthesis process, zeta potential and morphology size of SeNPs	700:765	The synthesis process, zeta potential and morphology size of SeNPs were changed by pH value, extract concentration and extract composition.					
34699821	2	17	contain	have	344:347	arg1	nanoparticles					289:301	selenium nanoparticles	280:301	selenium nanoparticles (SeNPs)	280:309	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	2	17	contain	have	344:347	arg1	SeNPs					304:308	SeNPs	304:308	SeNPs	304:308	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	2	17	contain	have	344:347	arg1	friendly					331:338	environmentally friendly	315:338	environmentally friendly	315:338	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	2	17	contain	have	344:347	arg2	prospects					371:379	promising application prospects	349:379	promising application prospects	349:379	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	5	18	theme	extract	793:799	arg1	concentration					801:813	extract concentration	793:813	extract concentration	793:813	The synthesis process, zeta potential and morphology size of SeNPs were changed by pH value, extract concentration and extract composition.					
34699821	10	19	theme	fulvic	1505:1510	arg1	substances					1522:1531	fulvic acid-like substances	1505:1531	fulvic acid-like substances	1505:1531	The present study demonstrated that proteins, polysaccharides, humic-like and fulvic acid-like substances cooperated in the formation and stabilization of SeNPs.					
34699821	13	20	theme	activated	1879:1887	arg1	sludge					1889:1894	activated sludge	1879:1894	activated sludge	1879:1894	This experiment provided a new insight into the resource utilization of activated sludge and a reference for the synthesis of nanometer selenium with excellent performance.					
34699821	5	21	theme	extract	819:825	arg1	composition					827:837	extract composition	819:837	extract composition	819:837	The synthesis process, zeta potential and morphology size of SeNPs were changed by pH value, extract concentration and extract composition.					
34699821	6	22	theme	same	850:853	arg1	conditions					865:874	the same synthesis conditions	846:874	the same synthesis conditions	846:874	Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo-spherical, while CSeNPs also contained pseudo-rod shape particles.					
34699821	9	23	from	contents	1277:1284	arg1	surfaces					1381:1388	their surfaces	1375:1388	their surfaces	1375:1388	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	10	24	theme	present	1431:1437	arg1	study					1439:1443	The present study	1427:1443	The present study	1427:1443	The present study demonstrated that proteins, polysaccharides, humic-like and fulvic acid-like substances cooperated in the formation and stabilization of SeNPs.					
34699821	2	25	theme	toxic	257:261	arg1	Selenite					243:250	Selenite	243:250	Selenite	243:250	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	2	25	theme	toxic	257:261	arg1	pollutant					263:271	a toxic pollutant	255:271	a toxic pollutant	255:271	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	7	26	dep	sizes	1019:1023	arg1	The					1015:1017	The	1015:1017	The	1015:1017	The sizes and crystal grains of CSeNPs were smaller than those of DSeNPs.					
34699821	4	27	theme	sludge	549:554	arg1	utilization					565:575	activated sludge resource utilization	539:575	activated sludge resource utilization	539:575	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	10	28	theme	acid-like	1512:1520	arg1	substances					1522:1531	fulvic acid-like substances	1505:1531	fulvic acid-like substances	1505:1531	The present study demonstrated that proteins, polysaccharides, humic-like and fulvic acid-like substances cooperated in the formation and stabilization of SeNPs.					
34699821	6	29	theme	synthesis	855:863	arg1	conditions					865:874	the same synthesis conditions	846:874	the same synthesis conditions	846:874	Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo-spherical, while CSeNPs also contained pseudo-rod shape particles.					
34699821	4	30	theme	activated	539:547	arg1	utilization					565:575	activated sludge resource utilization	539:575	activated sludge resource utilization	539:575	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	6	31	theme	DSeNPs	897:902	arg1	spherical					927:935	spherical	927:935	spherical	927:935	Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo-spherical, while CSeNPs also contained pseudo-rod shape particles.					
34699821	6	31	theme	DSeNPs	897:902	arg1	morphologies					881:892	the morphologies	877:892	the morphologies of DSeNPs and CSeNPs	877:913	Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo-spherical, while CSeNPs also contained pseudo-rod shape particles.					
34699821	3	32	theme	selenite	421:428	arg1	mechanism					408:416	the reduction mechanism	394:416	the reduction mechanism of selenite under the complex system	394:453	At present, the reduction mechanism of selenite under the complex system is still poorly understood.					
34699821	0	33	theme	photocatalytic	28:41	arg1	properties					43:52	photocatalytic properties	28:52	photocatalytic properties synthesized by residual activated sludge	28:93	Selenium nanoparticles with photocatalytic properties synthesized by residual activated sludge.					
34699821	13	34	theme	nanometer	1933:1941	arg1	selenium					1943:1950	nanometer selenium	1933:1950	nanometer selenium	1933:1950	This experiment provided a new insight into the resource utilization of activated sludge and a reference for the synthesis of nanometer selenium with excellent performance.					
34699821	5	35	theme	SeNPs	761:765	arg1	size					753:756	The synthesis process, zeta potential and morphology size	700:756	The synthesis process, zeta potential and morphology size of SeNPs	700:765	The synthesis process, zeta potential and morphology size of SeNPs were changed by pH value, extract concentration and extract composition.					
34699821	13	36	theme	new	1834:1836	arg1	insight					1838:1844	a new insight	1832:1844	a new insight into the resource utilization of activated sludge	1832:1894	This experiment provided a new insight into the resource utilization of activated sludge and a reference for the synthesis of nanometer selenium with excellent performance.					
34699821	4	37	theme	resource	556:563	arg1	utilization					565:575	activated sludge resource utilization	539:575	activated sludge resource utilization	539:575	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	7	38	theme	CSeNPs	1047:1052	arg1	sizes					1019:1023	sizes	1019:1023	sizes	1019:1023	The sizes and crystal grains of CSeNPs were smaller than those of DSeNPs.					
34699821	7	38	theme	CSeNPs	1047:1052	arg1	grains					1037:1042	crystal grains	1029:1042	crystal grains	1029:1042	The sizes and crystal grains of CSeNPs were smaller than those of DSeNPs.					
34699821	2	39	theme	selenium	280:287	arg1	nanoparticles					289:301	selenium nanoparticles	280:301	selenium nanoparticles (SeNPs)	280:309	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	2	39	theme	selenium	280:287	arg1	friendly					331:338	environmentally friendly	315:338	environmentally friendly	315:338	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	2	39	theme	selenium	280:287	arg1	SeNPs					304:308	SeNPs	304:308	SeNPs	304:308	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	13	40	theme	excellent	1957:1965	arg1	performance					1967:1977	excellent performance	1957:1977	excellent performance	1957:1977	This experiment provided a new insight into the resource utilization of activated sludge and a reference for the synthesis of nanometer selenium with excellent performance.					
34699821	4	41	theme	sewage	669:674	arg1	extracts					621:628	activated sludge extracts	604:628	activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs)	604:683	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	3	42	theme	reduction	398:406	arg1	mechanism					408:416	the reduction mechanism	394:416	the reduction mechanism of selenite under the complex system	394:453	At present, the reduction mechanism of selenite under the complex system is still poorly understood.					
34699821	2	43	theme	environmentally	315:329	arg1	nanoparticles					289:301	selenium nanoparticles	280:301	selenium nanoparticles (SeNPs)	280:309	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	2	43	theme	environmentally	315:329	arg1	friendly					331:338	environmentally friendly	315:338	environmentally friendly	315:338	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	7	44	theme	crystal	1029:1035	arg1	grains					1037:1042	crystal grains	1029:1042	crystal grains	1029:1042	The sizes and crystal grains of CSeNPs were smaller than those of DSeNPs.					
34699821	4	45	theme	sewage	642:647	arg1	extracts					621:628	activated sludge extracts	604:628	activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs)	604:683	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	9	46	dep	different	1405:1413	arg1	different					1405:1413	different	1405:1413	different	1405:1413	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	46	dep	different	1405:1413	arg1	contents					1277:1284	the relative contents	1264:1284	the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces	1264:1388	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	46	dep	different	1405:1413	arg1	P < 0.05					1416:1423	P < 0.05	1416:1423	P < 0.05	1416:1423	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	3	47	theme	complex	440:446	arg1	system					448:453	the complex system	436:453	the complex system	436:453	At present, the reduction mechanism of selenite under the complex system is still poorly understood.					
34699821	13	48	with	synthesis	1920:1928	arg1	performance					1967:1977	excellent performance	1957:1977	excellent performance	1957:1977	This experiment provided a new insight into the resource utilization of activated sludge and a reference for the synthesis of nanometer selenium with excellent performance.					
34699821	9	49	theme	α-d-glucopyranose	1299:1315	arg1	polysaccharides					1317:1331	α-d-glucopyranose polysaccharides	1299:1331	α-d-glucopyranose polysaccharides	1299:1331	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	11	50	theme	desirable	1637:1645	arg1	performance					1662:1672	more desirable photocatalytic performance	1632:1672	more desirable photocatalytic performance	1632:1672	Furthermore, CSeNPs (bandgap: 1.68 eV) had more desirable photocatalytic performance than DSeNPs (bandgap: 1.84 eV).					
34699821	4	51	theme	sludge	614:619	arg1	extracts					621:628	activated sludge extracts	604:628	activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs)	604:683	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	11	52	dep	DSeNPs	1679:1684	arg1	bandgap					1687:1693	bandgap	1687:1693	bandgap: 1.84 eV	1687:1702	Furthermore, CSeNPs (bandgap: 1.68 eV) had more desirable photocatalytic performance than DSeNPs (bandgap: 1.84 eV).					
34699821	4	53	theme	domestic	633:640	arg1	DSeNPs					650:655	DSeNPs	650:655	DSeNPs	650:655	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	4	53	theme	domestic	633:640	arg1	sewage					642:647	domestic sewage	633:647	domestic sewage (DSeNPs)	633:656	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	0	54	theme	residual	69:76	arg1	sludge					88:93	residual activated sludge	69:93	residual activated sludge	69:93	Selenium nanoparticles with photocatalytic properties synthesized by residual activated sludge.					
34699821	6	55	theme	CSeNPs	908:913	arg1	spherical					927:935	spherical	927:935	spherical	927:935	Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo-spherical, while CSeNPs also contained pseudo-rod shape particles.					
34699821	6	55	theme	CSeNPs	908:913	arg1	morphologies					881:892	the morphologies	877:892	the morphologies of DSeNPs and CSeNPs	877:913	Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo-spherical, while CSeNPs also contained pseudo-rod shape particles.					
34699821	5	56	theme	potential	728:736	arg1	size					753:756	The synthesis process, zeta potential and morphology size	700:756	The synthesis process, zeta potential and morphology size of SeNPs	700:765	The synthesis process, zeta potential and morphology size of SeNPs were changed by pH value, extract concentration and extract composition.					
34699821	8	57	located	found	1144:1148	arg2	~35 kDa					1131:1137	~35 kDa	1131:1137	~35 kDa	1131:1137	Compared with DSeNPs, a specific protein (~35 kDa) was found on the surface of CSeNPs using SDS-PAGE.					
34699821	8	57	located	found	1144:1148	arg1	surface					1157:1163	the surface	1153:1163	the surface of CSeNPs using SDS-PAGE	1153:1188	Compared with DSeNPs, a specific protein (~35 kDa) was found on the surface of CSeNPs using SDS-PAGE.					
34699821	8	57	located	found	1144:1148	arg2	protein					1122:1128	a specific protein	1111:1128	a specific protein (~35 kDa)	1111:1138	Compared with DSeNPs, a specific protein (~35 kDa) was found on the surface of CSeNPs using SDS-PAGE.					
34699821	1	58	theme	residual	126:133	arg1	sludge					145:150	residual activated sludge	126:150	residual activated sludge	126:150	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	10	59	theme	SeNPs	1582:1586	arg1	stabilization					1565:1577	stabilization	1565:1577	stabilization	1565:1577	The present study demonstrated that proteins, polysaccharides, humic-like and fulvic acid-like substances cooperated in the formation and stabilization of SeNPs.					
34699821	10	59	theme	SeNPs	1582:1586	arg1	formation					1551:1559	formation	1551:1559	formation	1551:1559	The present study demonstrated that proteins, polysaccharides, humic-like and fulvic acid-like substances cooperated in the formation and stabilization of SeNPs.					
34699821	4	60	theme	synthesis	526:534	arg1	mechanism					507:515	the mechanism	503:515	the mechanism of SeNPs synthesis by activated sludge resource utilization	503:575	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	12	61	theme	hydrogen	1788:1795	arg1	peroxide					1797:1804	hydrogen peroxide	1788:1804	hydrogen peroxide	1788:1804	Under the light condition, CSeNPs could degrade Rhodamine B faster without adding hydrogen peroxide.					
34699821	11	62	dep	bandgap	1610:1616	arg1	1.68 eV					1619:1625	1.68 eV	1619:1625	bandgap: 1.68 eV	1610:1625	Furthermore, CSeNPs (bandgap: 1.68 eV) had more desirable photocatalytic performance than DSeNPs (bandgap: 1.84 eV).					
34699821	5	63	theme	pH	783:784	arg1	value					786:790	pH value	783:790	pH value	783:790	The synthesis process, zeta potential and morphology size of SeNPs were changed by pH value, extract concentration and extract composition.					
34699821	1	64	theme	activated	135:143	arg1	sludge					145:150	residual activated sludge	126:150	residual activated sludge	126:150	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	4	65	theme	SeNPs	520:524	arg1	synthesis					526:534	SeNPs synthesis	520:534	SeNPs synthesis by activated sludge resource utilization	520:575	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	8	66	theme	specific	1113:1120	arg1	protein					1122:1128	a specific protein	1111:1128	a specific protein (~35 kDa)	1111:1138	Compared with DSeNPs, a specific protein (~35 kDa) was found on the surface of CSeNPs using SDS-PAGE.					
34699821	8	66	theme	specific	1113:1120	arg1	~35 kDa					1131:1137	~35 kDa	1131:1137	~35 kDa	1131:1137	Compared with DSeNPs, a specific protein (~35 kDa) was found on the surface of CSeNPs using SDS-PAGE.					
34699821	4	67	theme	coking	662:667	arg1	CSeNPs					677:682	CSeNPs	677:682	CSeNPs	677:682	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	4	67	theme	coking	662:667	arg1	sewage					669:674	coking sewage	662:674	coking sewage (CSeNPs)	662:683	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34699821	1	68	theme	sludge	145:150	arg1	problem					167:173	a worldwide problem	155:173	a worldwide problem	155:173	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	1	68	theme	sludge	145:150	arg1	disposal					114:121	disposal	114:121	disposal	114:121	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	1	68	theme	sludge	145:150	arg1	treatment					100:108	treatment	100:108	treatment	100:108	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	11	69	dep	desirable	1637:1645	arg1	photocatalytic					1647:1660	photocatalytic	1647:1660	photocatalytic	1647:1660	Furthermore, CSeNPs (bandgap: 1.68 eV) had more desirable photocatalytic performance than DSeNPs (bandgap: 1.84 eV).					
34699821	9	70	theme	polysaccharides	1317:1331	arg1	different					1405:1413	different	1405:1413	different	1405:1413	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	70	theme	polysaccharides	1317:1331	arg1	contents					1277:1284	the relative contents	1264:1284	the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces	1264:1388	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	70	theme	polysaccharides	1317:1331	arg1	P < 0.05					1416:1423	P < 0.05	1416:1423	P < 0.05	1416:1423	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	9	71	theme	fluorescence	1208:1219	arg1	images					1221:1226	the fluorescence images	1204:1226	the fluorescence images of the two SeNPs	1204:1243	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	13	72	theme	selenium	1943:1950	arg1	synthesis					1920:1928	the synthesis	1916:1928	the synthesis of nanometer selenium with excellent performance	1916:1977	This experiment provided a new insight into the resource utilization of activated sludge and a reference for the synthesis of nanometer selenium with excellent performance.					
34699821	1	73	theme	worldwide	157:165	arg1	disposal					114:121	disposal	114:121	disposal	114:121	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	1	73	theme	worldwide	157:165	arg1	problem					167:173	a worldwide problem	155:173	a worldwide problem	155:173	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	1	73	theme	worldwide	157:165	arg1	treatment					100:108	treatment	100:108	treatment	100:108	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	8	74	theme	CSeNPs	1168:1173	arg1	surface					1157:1163	the surface	1153:1163	the surface of CSeNPs using SDS-PAGE	1153:1188	Compared with DSeNPs, a specific protein (~35 kDa) was found on the surface of CSeNPs using SDS-PAGE.					
34699821	6	75	theme	shape	998:1002	arg1	particles					1004:1012	pseudo-rod shape particles	987:1012	pseudo-rod shape particles	987:1012	Under the same synthesis conditions, the morphologies of DSeNPs and CSeNPs were mainly spherical and pseudo-spherical, while CSeNPs also contained pseudo-rod shape particles.					
34699821	5	76	theme	zeta	723:726	arg1	potential					728:736	zeta potential	723:736	zeta potential	723:736	The synthesis process, zeta potential and morphology size of SeNPs were changed by pH value, extract concentration and extract composition.					
34699821	2	77	theme	application	359:369	arg1	prospects					371:379	promising application prospects	349:379	promising application prospects	349:379	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	13	78	theme	sludge	1889:1894	arg1	utilization					1864:1874	the resource utilization	1851:1874	the resource utilization of activated sludge	1851:1894	This experiment provided a new insight into the resource utilization of activated sludge and a reference for the synthesis of nanometer selenium with excellent performance.					
34699821	1	79	theme	earliest	226:233	arg1	stages					235:240	the earliest stages	222:240	the earliest stages	222:240	The treatment and disposal of residual activated sludge is a worldwide problem and the research on its reuse is still only in the earliest stages.					
34699821	0	80	theme	activated	78:86	arg1	sludge					88:93	residual activated sludge	69:93	residual activated sludge	69:93	Selenium nanoparticles with photocatalytic properties synthesized by residual activated sludge.					
34699821	9	81	theme	β-d-glucopyranose	1338:1354	arg1	polysaccharides					1356:1370	β-d-glucopyranose polysaccharides	1338:1370	β-d-glucopyranose polysaccharides	1338:1370	By analyzing the fluorescence images of the two SeNPs, it was found that the relative contents of proteins, α-d-glucopyranose polysaccharides, and β-d-glucopyranose polysaccharides on their surfaces were obviously different (P < 0.05).					
34699821	12	82	theme	Rhodamine	1754:1762	arg1	B					1764:1764	Rhodamine B	1754:1764	Rhodamine B	1754:1764	Under the light condition, CSeNPs could degrade Rhodamine B faster without adding hydrogen peroxide.					
34699821	2	83	theme	promising	349:357	arg1	prospects					371:379	promising application prospects	349:379	promising application prospects	349:379	Selenite is a toxic pollutant, while selenium nanoparticles (SeNPs) are environmentally friendly and have promising application prospects.					
34699821	4	84	theme	activated	604:612	arg1	extracts					621:628	activated sludge extracts	604:628	activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs)	604:683	In order to explore the mechanism of SeNPs synthesis by activated sludge resource utilization, SeNPs were synthesized by activated sludge extracts of domestic sewage (DSeNPs) and coking sewage (CSeNPs), respectively.					
34408181	12	0	theme	observed	1754:1761	arg1	action					1763:1768	The observed action	1750:1768	The observed action	1750:1768	The observed action of AAF, which targets not only the cell wall but also the mitochondria, makes the preparation a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis.					
34408181	7	1	theme	quantitative	1195:1206	arg1	analysis					1217:1224	relative quantitative SWATH MS analysis	1186:1224	relative quantitative SWATH MS analysis	1186:1224	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	12	2	theme	pathogen	1929:1936	arg1	cells					1904:1908	the cells	1900:1908	the cells of the C. albicans pathogen	1900:1936	The observed action of AAF, which targets not only the cell wall but also the mitochondria, makes the preparation a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis.					
34408181	6	3	theme	TEM	1140:1142	arg1	techniques					1152:1161	both the TEM and SEM techniques	1131:1161	techniques	1152:1161	The loss of the correct cell shape and cell wall integrity was visualized by both the TEM and SEM techniques.					
34408181	3	4	with	Staining	536:543	arg1	orange					572:577	acridine orange	563:577	acridine orange	563:577	Staining of the cells with acridine orange revealed a change in the pH of the AAF-treated cells.					
34408181	12	5	theme	C.	1917:1918	arg1	pathogen					1929:1936	the C. albicans pathogen	1913:1936	the C. albicans pathogen	1913:1936	The observed action of AAF, which targets not only the cell wall but also the mitochondria, makes the preparation a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis.					
34408181	6	6	theme	integrity	1103:1111	arg1	loss					1058:1061	The loss	1054:1061	The loss of the correct cell shape and cell wall integrity	1054:1111	The loss of the correct cell shape and cell wall integrity was visualized by both the TEM and SEM techniques.					
34408181	4	7	dep	migrated	702:709	arg1	both					746:749	both	746:749	both	746:749	It was observed that, after the AAF treatment, the mitochondrial DNA migrated towards the nuclear DNA, whereupon both merged into a single nuclear structure, which preceded the apoptotic process.					
34408181	9	8	theme	C.	1446:1447	arg1	cells					1458:1462	C. albicans cells	1446:1462	C. albicans cells caused by the action of AAF	1446:1490	The oxidative stress in C. albicans cells caused by the action of AAF was demonstrated by fluorescence microscopy, proteomic methods, and XPS spectroscopy.					
34408181	0	9	theme	veneta	131:136	arg1	fluid					147:151	Dendrobaena veneta coelomic fluid	119:151	Dendrobaena veneta coelomic fluid	119:151	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	1	10	theme	coelomic	214:221	arg1	fluid					223:227	the coelomic fluid	210:227	the coelomic fluid of Dendrobaena veneta earthworm	210:259	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	8	11	theme	stress	1405:1410	arg1	proteins					1412:1419	mitochondrial and oxidative stress proteins	1377:1419	mitochondrial and oxidative stress proteins	1377:1419	AAF was observed to influence the expression of mitochondrial and oxidative stress proteins.					
34408181	7	12	theme	AAF	1315:1317	arg1	fraction					1319:1326	the AAF fraction	1311:1326	the AAF fraction	1311:1326	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	0	13	theme	fluid	147:151	arg1	fraction					107:114	the protein-carbohydrate fraction	82:114	the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid	82:151	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	5	14	theme	transmission	1014:1025	arg1	microscopy					1036:1045	transmission electron microscopy	1014:1045	transmission electron microscopy (TEM)	1014:1051	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	5	14	theme	transmission	1014:1025	arg1	TEM					1048:1050	TEM	1048:1050	TEM	1048:1050	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	5	15	theme	electron	886:893	arg1	technique					921:929	the scanning electron cryomicroscopy (Cryo-SEM) technique	873:929	the scanning electron cryomicroscopy (Cryo-SEM) technique	873:929	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	3	16	theme	cells	626:630	arg1	pH					604:605	the pH	600:605	the pH of the AAF-treated cells	600:630	Staining of the cells with acridine orange revealed a change in the pH of the AAF-treated cells.					
34408181	9	17	theme	albicans	1449:1456	arg1	cells					1458:1462	C. albicans cells	1446:1462	C. albicans cells caused by the action of AAF	1446:1490	The oxidative stress in C. albicans cells caused by the action of AAF was demonstrated by fluorescence microscopy, proteomic methods, and XPS spectroscopy.					
34408181	6	18	theme	SEM	1148:1150	arg1	techniques					1152:1161	both the TEM and SEM techniques	1131:1161	techniques	1152:1161	The loss of the correct cell shape and cell wall integrity was visualized by both the TEM and SEM techniques.					
34408181	7	19	theme	relative	1186:1193	arg1	analysis					1217:1224	relative quantitative SWATH MS analysis	1186:1224	relative quantitative SWATH MS analysis	1186:1224	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	1	20	theme	isolated	158:165	arg1	AAF					200:202	AAF	200:202	AAF	200:202	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	1	20	theme	isolated	158:165	arg1	fraction					190:197	The isolated protein-polysaccharide fraction	154:197	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm	154:259	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	5	21	theme	enlarged	938:945	arg1	mitochondria					947:958	enlarged mitochondria	938:958	enlarged mitochondria	938:958	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	0	22	from	changes	37:43	arg1	cells					65:69	Candida albicans cells	48:69	Candida albicans cells	48:69	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	12	23	dep	C.	1917:1918	arg1	albicans					1920:1927	albicans	1920:1927	albicans	1920:1927	The observed action of AAF, which targets not only the cell wall but also the mitochondria, makes the preparation a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis.					
34408181	10	24	theme	AAF	1605:1607	arg1	proteins					1609:1616	AAF proteins	1605:1616	AAF proteins	1605:1616	The secondary structure of AAF proteins was characterized by Raman spectroscopy.					
34408181	0	25	theme	protein-carbohydrate	86:105	arg1	fraction					107:114	the protein-carbohydrate fraction	82:114	the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid	82:151	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	4	26	theme	nuclear	723:729	arg1	DNA					731:733	the nuclear DNA	719:733	the nuclear DNA	719:733	It was observed that, after the AAF treatment, the mitochondrial DNA migrated towards the nuclear DNA, whereupon both merged into a single nuclear structure, which preceded the apoptotic process.					
34408181	9	27	theme	proteomic	1537:1545	arg1	methods					1547:1553	proteomic methods	1537:1553	proteomic methods	1537:1553	The oxidative stress in C. albicans cells caused by the action of AAF was demonstrated by fluorescence microscopy, proteomic methods, and XPS spectroscopy.					
34408181	0	28	theme	Metabolic	0:8	arg1	changes					37:43	Metabolic, structural, and proteomic changes	0:43	Metabolic, structural, and proteomic changes in Candida albicans cells	0:69	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	9	29	theme	fluorescence	1512:1523	arg1	microscopy					1525:1534	fluorescence microscopy	1512:1534	fluorescence microscopy	1512:1534	The oxidative stress in C. albicans cells caused by the action of AAF was demonstrated by fluorescence microscopy, proteomic methods, and XPS spectroscopy.					
34408181	2	30	theme	morphological	456:468	arg1	forms					470:474	different morphological forms	446:474	different morphological forms	446:474	Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other.					
34408181	7	31	dep	C.	1269:1270	arg1	albicans					1272:1279	albicans	1272:1279	albicans	1272:1279	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	0	32	theme	structural	11:20	arg1	changes					37:43	Metabolic, structural, and proteomic changes	0:43	Metabolic, structural, and proteomic changes in Candida albicans cells	0:69	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	12	33	theme	cell	1805:1808	arg1	wall					1810:1813	the cell wall	1801:1813	not only the cell wall but also the mitochondria	1792:1839	The observed action of AAF, which targets not only the cell wall but also the mitochondria, makes the preparation a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis.					
34408181	7	34	used	used	1231:1234	arg2	spectrometry					1169:1180	Mass spectrometry	1164:1180	Mass spectrometry	1164:1180	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	7	34	used	used	1231:1234	arg2	analysis					1217:1224	relative quantitative SWATH MS analysis	1186:1224	relative quantitative SWATH MS analysis	1186:1224	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	2	35	theme	cell	428:431	arg1	division					433:440	disturbed cell division	418:440	disturbed cell division	418:440	Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other.					
34408181	12	36	theme	AAF	1773:1775	arg1	action					1763:1768	The observed action	1750:1768	The observed action	1750:1768	The observed action of AAF, which targets not only the cell wall but also the mitochondria, makes the preparation a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis.					
34408181	0	37	theme	Candida	48:54	arg1	albicans					56:63	Candida albicans	48:63	Candida albicans cells	48:69	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	11	38	theme	composition	1685:1695	arg1	Analysis					1659:1666	Analysis	1659:1666	Analysis of the elemental composition of AAF	1659:1702	Analysis of the elemental composition of AAF confirmed the homogeneity of the preparation.					
34408181	4	39	theme	single	765:770	arg1	structure					780:788	a single nuclear structure	763:788	a single nuclear structure	763:788	It was observed that, after the AAF treatment, the mitochondrial DNA migrated towards the nuclear DNA, whereupon both merged into a single nuclear structure, which preceded the apoptotic process.					
34408181	2	40	theme	clinical	338:345	arg1	strain					347:352	the clinical strain	334:352	the clinical strain after incubation with the active fraction	334:394	Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other.					
34408181	6	41	theme	cell	1078:1081	arg1	shape					1083:1087	the correct cell shape	1066:1087	the correct cell shape	1066:1087	The loss of the correct cell shape and cell wall integrity was visualized by both the TEM and SEM techniques.					
34408181	10	42	theme	proteins	1609:1616	arg1	structure					1592:1600	The secondary structure	1578:1600	The secondary structure of AAF proteins	1578:1616	The secondary structure of AAF proteins was characterized by Raman spectroscopy.					
34408181	8	43	theme	mitochondrial	1377:1389	arg1	proteins					1412:1419	mitochondrial and oxidative stress proteins	1377:1419	mitochondrial and oxidative stress proteins	1377:1419	AAF was observed to influence the expression of mitochondrial and oxidative stress proteins.					
34408181	2	44	theme	active	380:385	arg1	fraction					387:394	the active fraction	376:394	the active fraction	376:394	Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other.					
34408181	1	45	theme	Dendrobaena	232:242	arg1	earthworm					251:259	Dendrobaena veneta earthworm	232:259	Dendrobaena veneta earthworm	232:259	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	5	46	theme	cell	984:987	arg1	structures					989:998	cell structures	984:998	cell structures	984:998	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	1	47	theme	earthworm	251:259	arg1	fluid					223:227	the coelomic fluid	210:227	the coelomic fluid of Dendrobaena veneta earthworm	210:259	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	7	48	theme	SWATH	1208:1212	arg1	analysis					1217:1224	relative quantitative SWATH MS analysis	1186:1224	relative quantitative SWATH MS analysis	1186:1224	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	3	49	theme	cells	552:556	arg1	Staining					536:543	Staining	536:543	Staining of the cells with acridine orange	536:577	Staining of the cells with acridine orange revealed a change in the pH of the AAF-treated cells.					
34408181	1	50	theme	effective	267:275	arg1	activity					277:284	effective activity	267:284	effective activity against Candida albicans yeast	267:315	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	1	51	theme	protein-polysaccharide	167:188	arg1	AAF					200:202	AAF	200:202	AAF	200:202	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	1	51	theme	protein-polysaccharide	167:188	arg1	fraction					190:197	The isolated protein-polysaccharide fraction	154:197	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm	154:259	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	4	52	theme	apoptotic	810:818	arg1	process					820:826	the apoptotic process	806:826	the apoptotic process	806:826	It was observed that, after the AAF treatment, the mitochondrial DNA migrated towards the nuclear DNA, whereupon both merged into a single nuclear structure, which preceded the apoptotic process.					
34408181	5	53	theme	large	842:846	arg1	nucleus					848:854	a large nucleus	840:854	a large nucleus	840:854	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	0	54	theme	Dendrobaena	119:129	arg1	fluid					147:151	Dendrobaena veneta coelomic fluid	119:151	Dendrobaena veneta coelomic fluid	119:151	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	2	55	theme	Fungal	318:323	arg1	cells					325:329	Fungal cells	318:329	Fungal cells of the clinical strain after incubation with the active fraction	318:394	Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other.					
34408181	8	56	theme	oxidative	1395:1403	arg1	proteins					1412:1419	mitochondrial and oxidative stress proteins	1377:1419	mitochondrial and oxidative stress proteins	1377:1419	AAF was observed to influence the expression of mitochondrial and oxidative stress proteins.					
34408181	6	57	theme	wall	1098:1101	arg1	integrity					1103:1111	cell wall integrity	1093:1111	cell wall integrity	1093:1111	The loss of the correct cell shape and cell wall integrity was visualized by both the TEM and SEM techniques.					
34408181	0	58	theme	coelomic	138:145	arg1	fluid					147:151	Dendrobaena veneta coelomic fluid	119:151	Dendrobaena veneta coelomic fluid	119:151	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	9	59	theme	oxidative	1426:1434	arg1	stress					1436:1441	The oxidative stress	1422:1441	The oxidative stress in C. albicans cells caused by the action of AAF	1422:1490	The oxidative stress in C. albicans cells caused by the action of AAF was demonstrated by fluorescence microscopy, proteomic methods, and XPS spectroscopy.					
34408181	12	60	theme	antifungal	1876:1885	arg1	drug					1887:1890	a potential antifungal drug	1864:1890	a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis	1864:1954	The observed action of AAF, which targets not only the cell wall but also the mitochondria, makes the preparation a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis.					
34408181	8	61	theme	proteins	1412:1419	arg1	expression					1363:1372	the expression	1359:1372	the expression of mitochondrial and oxidative stress proteins	1359:1419	AAF was observed to influence the expression of mitochondrial and oxidative stress proteins.					
34408181	5	62	theme	scanning	877:884	arg1	technique					921:929	the scanning electron cryomicroscopy (Cryo-SEM) technique	873:929	the scanning electron cryomicroscopy (Cryo-SEM) technique	873:929	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	2	63	from	other	529:533	arg1	separate					500:507	separate	500:507	separate	500:507	Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other.					
34408181	7	64	theme	fraction	1319:1326	arg1	components					1297:1306	the components	1293:1306	the components of the AAF fraction	1293:1326	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	4	65	theme	mitochondrial	684:696	arg1	DNA					698:700	the mitochondrial DNA	680:700	the mitochondrial DNA	680:700	It was observed that, after the AAF treatment, the mitochondrial DNA migrated towards the nuclear DNA, whereupon both merged into a single nuclear structure, which preceded the apoptotic process.					
34408181	1	66	from	fluid	223:227	arg1	AAF					200:202	AAF	200:202	AAF	200:202	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	1	66	from	fluid	223:227	arg1	fraction					190:197	The isolated protein-polysaccharide fraction	154:197	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm	154:259	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	5	67	theme	electron	1027:1034	arg1	microscopy					1036:1045	transmission electron microscopy	1014:1045	transmission electron microscopy (TEM)	1014:1051	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	5	67	theme	electron	1027:1034	arg1	TEM					1048:1050	TEM	1048:1050	TEM	1048:1050	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	3	68	theme	AAF-treated	614:624	arg1	cells					626:630	the AAF-treated cells	610:630	the AAF-treated cells	610:630	Staining of the cells with acridine orange revealed a change in the pH of the AAF-treated cells.					
34408181	5	69	theme	cryomicroscopy	895:908	arg1	technique					921:929	the scanning electron cryomicroscopy (Cryo-SEM) technique	873:929	the scanning electron cryomicroscopy (Cryo-SEM) technique	873:929	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	3	70	theme	acridine	563:570	arg1	orange					572:577	acridine orange	563:577	acridine orange	563:577	Staining of the cells with acridine orange revealed a change in the pH of the AAF-treated cells.					
34408181	0	71	theme	albicans	56:63	arg1	cells					65:69	Candida albicans cells	48:69	Candida albicans cells	48:69	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	1	72	dep	albicans	302:309	arg1	yeast					311:315	yeast	311:315	Candida albicans yeast	294:315	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	4	73	theme	AAF	665:667	arg1	treatment					669:677	the AAF treatment	661:677	the AAF treatment	661:677	It was observed that, after the AAF treatment, the mitochondrial DNA migrated towards the nuclear DNA, whereupon both merged into a single nuclear structure, which preceded the apoptotic process.					
34408181	11	74	theme	AAF	1700:1702	arg1	composition					1685:1695	the elemental composition	1671:1695	the elemental composition of AAF	1671:1702	Analysis of the elemental composition of AAF confirmed the homogeneity of the preparation.					
34408181	7	75	theme	Mass	1164:1167	arg1	spectrometry					1169:1180	Mass spectrometry	1164:1180	Mass spectrometry	1164:1180	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	11	76	theme	preparation	1737:1747	arg1	homogeneity					1718:1728	the homogeneity	1714:1728	the homogeneity of the preparation	1714:1747	Analysis of the elemental composition of AAF confirmed the homogeneity of the preparation.					
34408181	12	77	theme	potential	1866:1874	arg1	drug					1887:1890	a potential antifungal drug	1864:1890	a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis	1864:1954	The observed action of AAF, which targets not only the cell wall but also the mitochondria, makes the preparation a potential antifungal drug killing the cells of the C. albicans pathogen through apoptosis.					
34408181	9	78	theme	AAF	1488:1490	arg1	action					1478:1483	the action	1474:1483	the action of AAF	1474:1490	The oxidative stress in C. albicans cells caused by the action of AAF was demonstrated by fluorescence microscopy, proteomic methods, and XPS spectroscopy.					
34408181	7	79	theme	proteome	1281:1288	arg1	reaction					1253:1260	the reaction	1249:1260	the reaction of the C. albicans proteome to the components of the AAF fraction	1249:1326	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	11	80	gly	homogeneity	1718:1728	arg1	preparation					1737:1747	the preparation	1733:1747	the preparation	1733:1747	Analysis of the elemental composition of AAF confirmed the homogeneity of the preparation.					
34408181	5	81	theme	Cryo-SEM	911:918	arg1	technique					921:929	the scanning electron cryomicroscopy (Cryo-SEM) technique	873:929	the scanning electron cryomicroscopy (Cryo-SEM) technique	873:929	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	7	82	theme	C.	1269:1270	arg1	proteome					1281:1288	the C. albicans proteome	1265:1288	the C. albicans proteome	1265:1288	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	3	83	from	change	590:595	arg1	pH					604:605	the pH	600:605	the pH of the AAF-treated cells	600:630	Staining of the cells with acridine orange revealed a change in the pH of the AAF-treated cells.					
34408181	10	84	theme	secondary	1582:1590	arg1	structure					1592:1600	The secondary structure	1578:1600	The secondary structure of AAF proteins	1578:1616	The secondary structure of AAF proteins was characterized by Raman spectroscopy.					
34408181	1	85	theme	Candida	294:300	arg1	albicans					302:309	Candida albicans yeast	294:315	Candida albicans yeast	294:315	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34408181	2	86	theme	different	446:454	arg1	forms					470:474	different morphological forms	446:474	different morphological forms	446:474	Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other.					
34408181	5	87	with	Cells	829:833	arg1	nucleus					848:854	a large nucleus	840:854	a large nucleus	840:854	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	7	88	theme	MS	1214:1215	arg1	analysis					1217:1224	relative quantitative SWATH MS analysis	1186:1224	relative quantitative SWATH MS analysis	1186:1224	Mass spectrometry and relative quantitative SWATH MS analysis were used to determine the reaction of the C. albicans proteome to the components of the AAF fraction.					
34408181	0	89	theme	proteomic	27:35	arg1	changes					37:43	Metabolic, structural, and proteomic changes	0:43	Metabolic, structural, and proteomic changes in Candida albicans cells	0:69	Metabolic, structural, and proteomic changes in Candida albicans cells induced by the protein-carbohydrate fraction of Dendrobaena veneta coelomic fluid.					
34408181	5	90	theme	structures	989:998	arg1	mitochondria					947:958	enlarged mitochondria	938:958	enlarged mitochondria	938:958	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	5	90	theme	structures	989:998	arg1	degeneration					968:979	the degeneration	964:979	the degeneration of cell structures	964:998	Cells with a large nucleus were imaged with the scanning electron cryomicroscopy (Cryo-SEM) technique, while enlarged mitochondria and the degeneration of cell structures were shown by transmission electron microscopy (TEM).					
34408181	2	91	theme	disturbed	418:426	arg1	division					433:440	disturbed cell division	418:440	disturbed cell division	418:440	Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other.					
34408181	9	92	from	stress	1436:1441	arg1	cells					1458:1462	C. albicans cells	1446:1462	C. albicans cells caused by the action of AAF	1446:1490	The oxidative stress in C. albicans cells caused by the action of AAF was demonstrated by fluorescence microscopy, proteomic methods, and XPS spectroscopy.					
34408181	6	93	theme	cell	1093:1096	arg1	integrity					1103:1111	cell wall integrity	1093:1111	cell wall integrity	1093:1111	The loss of the correct cell shape and cell wall integrity was visualized by both the TEM and SEM techniques.					
34408181	11	94	theme	elemental	1675:1683	arg1	composition					1685:1695	the elemental composition	1671:1695	the elemental composition of AAF	1671:1702	Analysis of the elemental composition of AAF confirmed the homogeneity of the preparation.					
34408181	2	95	theme	strain	347:352	arg1	cells					325:329	Fungal cells	318:329	Fungal cells of the clinical strain after incubation with the active fraction	318:394	Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other.					
34408181	2	96	with	incubation	360:369	arg1	fraction					387:394	the active fraction	376:394	the active fraction	376:394	Fungal cells of the clinical strain after incubation with the active fraction were characterized by disturbed cell division and different morphological forms due to the inability to separate the cells from each other.					
34408181	9	97	theme	XPS	1560:1562	arg1	spectroscopy					1564:1575	XPS spectroscopy	1560:1575	XPS spectroscopy	1560:1575	The oxidative stress in C. albicans cells caused by the action of AAF was demonstrated by fluorescence microscopy, proteomic methods, and XPS spectroscopy.					
34408181	6	98	theme	shape	1083:1087	arg1	loss					1058:1061	The loss	1054:1061	The loss of the correct cell shape and cell wall integrity	1054:1111	The loss of the correct cell shape and cell wall integrity was visualized by both the TEM and SEM techniques.					
34408181	10	99	theme	Raman	1639:1643	arg1	spectroscopy					1645:1656	Raman spectroscopy	1639:1656	Raman spectroscopy	1639:1656	The secondary structure of AAF proteins was characterized by Raman spectroscopy.					
34408181	6	100	theme	correct	1070:1076	arg1	shape					1083:1087	the correct cell shape	1066:1087	the correct cell shape	1066:1087	The loss of the correct cell shape and cell wall integrity was visualized by both the TEM and SEM techniques.					
34408181	4	101	theme	nuclear	772:778	arg1	structure					780:788	a single nuclear structure	763:788	a single nuclear structure	763:788	It was observed that, after the AAF treatment, the mitochondrial DNA migrated towards the nuclear DNA, whereupon both merged into a single nuclear structure, which preceded the apoptotic process.					
34408181	1	102	theme	veneta	244:249	arg1	earthworm					251:259	Dendrobaena veneta earthworm	232:259	Dendrobaena veneta earthworm	232:259	The isolated protein-polysaccharide fraction (AAF) from the coelomic fluid of Dendrobaena veneta earthworm shows effective activity against Candida albicans yeast.					
34742438	0	0	theme	hydrogen	105:112	arg1	reconstruction					119:132	hydrogen bond reconstruction	105:132	hydrogen bond reconstruction	105:132	Simultaneous toughening and strengthening of chitin-based composites via tensile-induced orientation and hydrogen bond reconstruction.					
34742438	4	1	theme	film	739:742	arg1	elongation					702:711	elongation	702:711	elongation	702:711	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	4	1	theme	film	739:742	arg1	strength					689:696	tensile strength	681:696	tensile strength	681:696	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	5	2	theme	intra-layer	927:937	arg1	orientation					939:949	intra-layer orientation	927:949	intra-layer orientation	927:949	Furthermore, strengthening and toughening mechanisms were also studied, which were attributed to the effects of the intra-layer orientation and interlayer sliding, respectively.					
34742438	1	3	theme	biocompatible	175:187	arg1	polysaccharide					189:202	an abundant, biodegradable, and biocompatible polysaccharide	143:202	an abundant, biodegradable, and biocompatible polysaccharide	143:202	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	1	3	theme	biocompatible	175:187	arg1	Chitin					135:140	Chitin	135:140	Chitin	135:140	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	2	4	theme	low	359:361	arg1	processability					363:376	their low processability	353:376	their low processability	353:376	However, the applications of chitin-based materials are hindered by their low processability and brittleness induced by strong hydrogen bonds.					
34742438	3	5	theme	alcohol	568:574	arg1	film					587:590	a chitin nanowhiskers/poly(vinyl alcohol) composite film	535:590	a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness	535:623	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	2	6	theme	chitin-based	314:325	arg1	materials					327:335	chitin-based materials	314:335	chitin-based materials	314:335	However, the applications of chitin-based materials are hindered by their low processability and brittleness induced by strong hydrogen bonds.					
34742438	4	7	theme	hydrogen	647:654	arg1	bond					656:659	hydrogen bond	647:659	hydrogen bond reconstruction	647:674	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	0	8	theme	bond	114:117	arg1	reconstruction					119:132	hydrogen bond reconstruction	105:132	hydrogen bond reconstruction	105:132	Simultaneous toughening and strengthening of chitin-based composites via tensile-induced orientation and hydrogen bond reconstruction.					
34742438	3	9	theme	high	597:600	arg1	strength					602:609	high strength	597:609	high strength	597:609	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	5	10	dep	strengthening	824:836	arg1	mechanisms					853:862	mechanisms	853:862	mechanisms	853:862	Furthermore, strengthening and toughening mechanisms were also studied, which were attributed to the effects of the intra-layer orientation and interlayer sliding, respectively.					
34742438	4	11	theme	tensile	681:687	arg1	strength					689:696	tensile strength	681:696	tensile strength	681:696	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	3	12	theme	composite	577:585	arg1	film					587:590	a chitin nanowhiskers/poly(vinyl alcohol) composite film	535:590	a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness	535:623	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	5	13	theme	sliding	966:972	arg1	effects					912:918	the effects	908:918	the effects of the intra-layer orientation and interlayer sliding, respectively	908:986	Furthermore, strengthening and toughening mechanisms were also studied, which were attributed to the effects of the intra-layer orientation and interlayer sliding, respectively.					
34742438	3	14	theme	bond	479:482	arg1	strategy					499:506	a tensile-induced orientation and hydrogen bond reconstruction strategy	436:506	a tensile-induced orientation and hydrogen bond reconstruction strategy	436:506	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	5	15	theme	orientation	939:949	arg1	effects					912:918	the effects	908:918	the effects of the intra-layer orientation and interlayer sliding, respectively	908:986	Furthermore, strengthening and toughening mechanisms were also studied, which were attributed to the effects of the intra-layer orientation and interlayer sliding, respectively.					
34742438	3	16	theme	tensile-induced	438:452	arg1	strategy					499:506	a tensile-induced orientation and hydrogen bond reconstruction strategy	436:506	a tensile-induced orientation and hydrogen bond reconstruction strategy	436:506	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	4	17	theme	bond	656:659	arg1	reconstruction					661:674	hydrogen bond reconstruction	647:674	hydrogen bond reconstruction	647:674	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	4	18	from	break	716:720	arg1	elongation					702:711	elongation	702:711	elongation	702:711	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	4	18	from	break	716:720	arg1	strength					689:696	tensile strength	681:696	tensile strength	681:696	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	3	19	theme	reconstruction	484:497	arg1	strategy					499:506	a tensile-induced orientation and hydrogen bond reconstruction strategy	436:506	a tensile-induced orientation and hydrogen bond reconstruction strategy	436:506	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	4	20	theme	%	785:785	arg1	%					794:794	9.37% to 40.7%	781:794	9.37% to 40.7%	781:794	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	0	21	theme	Simultaneous	0:11	arg1	toughening					13:22	Simultaneous toughening	0:22	Simultaneous toughening	0:22	Simultaneous toughening and strengthening of chitin-based composites via tensile-induced orientation and hydrogen bond reconstruction.					
34742438	5	22	dep	orientation	939:949	arg1	the					923:925	the	923:925	the	923:925	Furthermore, strengthening and toughening mechanisms were also studied, which were attributed to the effects of the intra-layer orientation and interlayer sliding, respectively.					
34742438	2	23	theme	hydrogen	412:419	arg1	bonds					421:425	strong hydrogen bonds	405:425	strong hydrogen bonds	405:425	However, the applications of chitin-based materials are hindered by their low processability and brittleness induced by strong hydrogen bonds.					
34742438	2	24	theme	materials	327:335	arg1	applications					298:309	the applications	294:309	the applications of chitin-based materials	294:335	However, the applications of chitin-based materials are hindered by their low processability and brittleness induced by strong hydrogen bonds.					
34742438	1	25	theme	biodegradable	156:168	arg1	polysaccharide					189:202	an abundant, biodegradable, and biocompatible polysaccharide	143:202	an abundant, biodegradable, and biocompatible polysaccharide	143:202	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	1	25	theme	biodegradable	156:168	arg1	Chitin					135:140	Chitin	135:140	Chitin	135:140	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	0	26	theme	chitin-based	45:56	arg1	composites					58:67	chitin-based composites	45:67	chitin-based composites	45:67	Simultaneous toughening and strengthening of chitin-based composites via tensile-induced orientation and hydrogen bond reconstruction.					
34742438	5	27	theme	interlayer	955:964	arg1	sliding					966:972	interlayer sliding	955:972	interlayer sliding	955:972	Furthermore, strengthening and toughening mechanisms were also studied, which were attributed to the effects of the intra-layer orientation and interlayer sliding, respectively.					
34742438	3	28	theme	hydrogen	470:477	arg1	bond					479:482	hydrogen bond	470:482	hydrogen bond	470:482	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	1	29	theme	most	219:222	arg1	alternatives					243:254	the most ideal eco-friendly alternatives	215:254	the most ideal eco-friendly alternatives to petroleum-based plastics	215:282	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	3	30	theme	orientation	454:464	arg1	strategy					499:506	a tensile-induced orientation and hydrogen bond reconstruction strategy	436:506	a tensile-induced orientation and hydrogen bond reconstruction strategy	436:506	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	3	31	with	film	587:590	arg1	toughness					615:623	toughness	615:623	toughness	615:623	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	3	31	with	film	587:590	arg1	strength					602:609	high strength	597:609	high strength	597:609	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	1	32	theme	ideal	224:228	arg1	alternatives					243:254	the most ideal eco-friendly alternatives	215:254	the most ideal eco-friendly alternatives to petroleum-based plastics	215:282	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	4	33	dep	strength	689:696	arg1	the					677:679	the	677:679	the	677:679	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	3	34	theme	chitin	537:542	arg1	film					587:590	a chitin nanowhiskers/poly(vinyl alcohol) composite film	535:590	a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness	535:623	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	0	35	theme	composites	58:67	arg1	strengthening					28:40	strengthening	28:40	strengthening	28:40	Simultaneous toughening and strengthening of chitin-based composites via tensile-induced orientation and hydrogen bond reconstruction.					
34742438	0	35	theme	composites	58:67	arg1	toughening					13:22	Simultaneous toughening	0:22	Simultaneous toughening	0:22	Simultaneous toughening and strengthening of chitin-based composites via tensile-induced orientation and hydrogen bond reconstruction.					
34742438	3	36	theme	vinyl	562:566	arg1	film					587:590	a chitin nanowhiskers/poly(vinyl alcohol) composite film	535:590	a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness	535:623	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	3	37	theme	nanowhiskers/poly	544:560	arg1	film					587:590	a chitin nanowhiskers/poly(vinyl alcohol) composite film	535:590	a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness	535:623	Herein, a tensile-induced orientation and hydrogen bond reconstruction strategy was developed to fabricate a chitin nanowhiskers/poly(vinyl alcohol) composite film with high strength and toughness.					
34742438	4	38	dep	%	794:794	arg1	to					787:788	to	787:788	to	787:788	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	1	39	theme	abundant	146:153	arg1	polysaccharide					189:202	an abundant, biodegradable, and biocompatible polysaccharide	143:202	an abundant, biodegradable, and biocompatible polysaccharide	143:202	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	1	39	theme	abundant	146:153	arg1	Chitin					135:140	Chitin	135:140	Chitin	135:140	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	1	40	theme	eco-friendly	230:241	arg1	alternatives					243:254	the most ideal eco-friendly alternatives	215:254	the most ideal eco-friendly alternatives to petroleum-based plastics	215:282	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	1	41	theme	alternatives	243:254	arg1	one					208:210	one	208:210	one	208:210	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	1	41	theme	alternatives	243:254	arg1	alternatives					243:254	the most ideal eco-friendly alternatives	215:254	the most ideal eco-friendly alternatives to petroleum-based plastics	215:282	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
34742438	2	42	theme	strong	405:410	arg1	bonds					421:425	strong hydrogen bonds	405:425	strong hydrogen bonds	405:425	However, the applications of chitin-based materials are hindered by their low processability and brittleness induced by strong hydrogen bonds.					
34742438	0	43	theme	tensile-induced	73:87	arg1	orientation					89:99	tensile-induced orientation	73:99	tensile-induced orientation	73:99	Simultaneous toughening and strengthening of chitin-based composites via tensile-induced orientation and hydrogen bond reconstruction.					
34742438	4	44	theme	composite	729:737	arg1	film					739:742	the composite film	725:742	the composite film	725:742	After stretching and hydrogen bond reconstruction, the tensile strength and elongation at break of the composite film increased from 38.6 to 115.2 MPa and 9.37% to 40.7%, respectively.					
34742438	1	45	theme	petroleum-based	259:273	arg1	plastics					275:282	petroleum-based plastics	259:282	petroleum-based plastics	259:282	Chitin, an abundant, biodegradable, and biocompatible polysaccharide, is one of the most ideal eco-friendly alternatives to petroleum-based plastics.					
33095019	5	0	theme	bowel	1400:1404	arg1	diseases					1406:1413	inflammatory bowel diseases	1387:1413	inflammatory bowel diseases	1387:1413	The key microorganisms, including Muribaculaceae, Lachnospiraceae, Lachnospiraceae_NK4A136_group, Mucispirillum, Helicobacter, Ruminococcus_1, and Ruminiclostridium_5, were identified to be associated with inflammatory bowel diseases.					
33095019	4	1	dep	increased	784:792	arg1	1					656:656	1	656:656	1	656:656	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	2	theme	histological	836:847	arg1	score					849:853	the histological score	832:853	the histological score of colitic mice	832:869	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	3	dep	WSGP	895:898	arg1	2					888:888	2	888:888	2	888:888	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	3	dep	WSGP	895:898	arg1	reduced					900:906	reduced	900:906	reduced the colonic tissue damage	900:932	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	3	dep	WSGP	895:898	arg1	inhibited					938:946	inhibited	938:946	inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha)	938:1055	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	5	4	theme	key	1185:1187	arg1	Ruminococcus_1					1308:1321	Ruminococcus_1	1308:1321	Ruminococcus_1	1308:1321	The key microorganisms, including Muribaculaceae, Lachnospiraceae, Lachnospiraceae_NK4A136_group, Mucispirillum, Helicobacter, Ruminococcus_1, and Ruminiclostridium_5, were identified to be associated with inflammatory bowel diseases.					
33095019	5	4	theme	key	1185:1187	arg1	microorganisms					1189:1202	The key microorganisms	1181:1202	The key microorganisms	1181:1202	The key microorganisms, including Muribaculaceae, Lachnospiraceae, Lachnospiraceae_NK4A136_group, Mucispirillum, Helicobacter, Ruminococcus_1, and Ruminiclostridium_5, were identified to be associated with inflammatory bowel diseases.					
33095019	5	4	theme	key	1185:1187	arg1	Lachnospiraceae					1231:1245	Lachnospiraceae	1231:1245	Lachnospiraceae	1231:1245	The key microorganisms, including Muribaculaceae, Lachnospiraceae, Lachnospiraceae_NK4A136_group, Mucispirillum, Helicobacter, Ruminococcus_1, and Ruminiclostridium_5, were identified to be associated with inflammatory bowel diseases.					
33095019	5	4	theme	key	1185:1187	arg1	Ruminiclostridium_5					1328:1346	Ruminiclostridium_5	1328:1346	Ruminiclostridium_5	1328:1346	The key microorganisms, including Muribaculaceae, Lachnospiraceae, Lachnospiraceae_NK4A136_group, Mucispirillum, Helicobacter, Ruminococcus_1, and Ruminiclostridium_5, were identified to be associated with inflammatory bowel diseases.					
33095019	5	4	theme	key	1185:1187	arg1	Mucispirillum					1279:1291	Mucispirillum	1279:1291	Mucispirillum	1279:1291	The key microorganisms, including Muribaculaceae, Lachnospiraceae, Lachnospiraceae_NK4A136_group, Mucispirillum, Helicobacter, Ruminococcus_1, and Ruminiclostridium_5, were identified to be associated with inflammatory bowel diseases.					
33095019	4	5	theme	colitic	858:864	arg1	mice					866:869	colitic mice	858:869	colitic mice	858:869	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	6	theme	colon	750:754	arg1	length					756:761	colon length	750:761	colon length	750:761	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	7	theme	WSGP	687:690	arg1	administration					665:678	administration	665:678	administration of the WSGP (200 or 400 mg/kg/day)	665:713	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	3	8	theme	water-soluble	450:462	arg1	WSGP					487:490	WSGP	487:490	WSGP	487:490	Herein, a water-soluble garlic polysaccharide (WSGP) was extracted from Jinxiang garlic to evaluate its effects on ameliorating dextran sulfate sodium (DSS)-induced colitis in a mouse model.					
33095019	3	8	theme	water-soluble	450:462	arg1	polysaccharide					471:484	a water-soluble garlic polysaccharide	448:484	a water-soluble garlic polysaccharide (WSGP)	448:491	Herein, a water-soluble garlic polysaccharide (WSGP) was extracted from Jinxiang garlic to evaluate its effects on ameliorating dextran sulfate sodium (DSS)-induced colitis in a mouse model.					
33095019	4	9	theme	factors	979:985	arg1	expression					952:961	the expression	948:961	the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha)	948:1055	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	10	theme	microbiota	1169:1178	arg1	composition					1143:1153	the composition	1139:1153	the composition of intestinal microbiota	1139:1178	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	3	11	theme	-induced	596:603	arg1	colitis					605:611	dextran sulfate sodium (DSS)-induced colitis	568:611	dextran sulfate sodium (DSS)-induced colitis in a mouse model	568:628	Herein, a water-soluble garlic polysaccharide (WSGP) was extracted from Jinxiang garlic to evaluate its effects on ameliorating dextran sulfate sodium (DSS)-induced colitis in a mouse model.					
33095019	4	12	theme	feed	720:723	arg1	intake					725:730	the feed intake	716:730	the feed intake	716:730	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	13	theme	colonic	912:918	arg1	damage					927:932	the colonic tissue damage	908:932	the colonic tissue damage	908:932	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	14	theme	intestinal	1158:1167	arg1	microbiota					1169:1178	intestinal microbiota	1158:1178	intestinal microbiota	1158:1178	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	6	15	theme	mucosal	1525:1531	arg1	barriers					1533:1540	mucosal barriers	1525:1540	mucosal barriers	1525:1540	Taken together, this study proved that WSGP supplementation could alleviate DSS-induced colitis by improving mucosal barriers, blocking proinflammatory cytokines, and modulating gut microbiota.					
33095019	1	16	theme	great	195:199	arg1	agents					211:216	great potential agents	195:216	great potential agents	195:216	Garlic polysaccharides are great potential agents because of their anti-inflammation, antioxidation, and immunomodulation properties.					
33095019	1	16	theme	great	195:199	arg1	polysaccharides					175:189	Garlic polysaccharides	168:189	Garlic polysaccharides	168:189	Garlic polysaccharides are great potential agents because of their anti-inflammation, antioxidation, and immunomodulation properties.					
33095019	0	17	theme	Polysaccharides	105:119	arg1	Properties					55:64	Anti-Inflammatory and Intestinal Microbiota Modulation Properties	0:64	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.	0:166	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.					
33095019	4	18	theme	activity	813:820	arg1	index					822:826	the disease activity index	801:826	the disease activity index	801:826	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	2	19	theme	few	311:313	arg1	studies					315:321	few studies	311:321	few studies	311:321	However, few studies have reported their anti-inflammatory effects on improving the colon system and corresponding intestinal microbiota.					
33095019	1	20	theme	potential	201:209	arg1	agents					211:216	great potential agents	195:216	great potential agents	195:216	Garlic polysaccharides are great potential agents because of their anti-inflammation, antioxidation, and immunomodulation properties.					
33095019	1	20	theme	potential	201:209	arg1	polysaccharides					175:189	Garlic polysaccharides	168:189	Garlic polysaccharides	168:189	Garlic polysaccharides are great potential agents because of their anti-inflammation, antioxidation, and immunomodulation properties.					
33095019	4	21	theme	acids	1120:1124	arg1	production					1088:1097	the production	1084:1097	the production of short-chain fatty acids	1084:1124	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	22	theme	body	733:736	arg1	weight					738:743	body weight	733:743	body weight	733:743	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	0	23	theme	Anti-Inflammatory	0:16	arg1	Properties					55:64	Anti-Inflammatory and Intestinal Microbiota Modulation Properties	0:64	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.	0:166	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.					
33095019	4	24	dep	factors	979:985	arg1	beta					1017:1020	interleukin 1 beta	1003:1020	interleukin 1 beta	1003:1020	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	24	dep	factors	979:985	arg1	interleukin					988:998	interleukin 6	988:1000	interleukin 6	988:1000	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	24	dep	factors	979:985	arg1	alpha					1050:1054	tumor necrosis factor alpha	1028:1054	tumor necrosis factor alpha	1028:1054	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	24	dep	factors	979:985	arg1	factors					979:985	inflammatory factors	966:985	inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha)	966:1055	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	25	theme	inflammatory	966:977	arg1	beta					1017:1020	interleukin 1 beta	1003:1020	interleukin 1 beta	1003:1020	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	25	theme	inflammatory	966:977	arg1	interleukin					988:998	interleukin 6	988:1000	interleukin 6	988:1000	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	25	theme	inflammatory	966:977	arg1	alpha					1050:1054	tumor necrosis factor alpha	1028:1054	tumor necrosis factor alpha	1028:1054	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	25	theme	inflammatory	966:977	arg1	factors					979:985	inflammatory factors	966:985	inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha)	966:1055	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	3	26	theme	mouse	618:622	arg1	model					624:628	a mouse model	616:628	a mouse model	616:628	Herein, a water-soluble garlic polysaccharide (WSGP) was extracted from Jinxiang garlic to evaluate its effects on ameliorating dextran sulfate sodium (DSS)-induced colitis in a mouse model.					
33095019	0	27	theme	Microbiota	33:42	arg1	Properties					55:64	Anti-Inflammatory and Intestinal Microbiota Modulation Properties	0:64	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.	0:166	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.					
33095019	5	28	theme	inflammatory	1387:1398	arg1	diseases					1406:1413	inflammatory bowel diseases	1387:1413	inflammatory bowel diseases	1387:1413	The key microorganisms, including Muribaculaceae, Lachnospiraceae, Lachnospiraceae_NK4A136_group, Mucispirillum, Helicobacter, Ruminococcus_1, and Ruminiclostridium_5, were identified to be associated with inflammatory bowel diseases.					
33095019	2	29	theme	anti-inflammatory	343:359	arg1	effects					361:367	their anti-inflammatory effects	337:367	their anti-inflammatory effects	337:367	However, few studies have reported their anti-inflammatory effects on improving the colon system and corresponding intestinal microbiota.					
33095019	4	30	theme	factor	1043:1048	arg1	alpha					1050:1054	tumor necrosis factor alpha	1028:1054	tumor necrosis factor alpha	1028:1054	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	30	theme	factor	1043:1048	arg1	factors					979:985	inflammatory factors	966:985	inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha)	966:1055	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	0	31	dep	Allium	86:91	arg1	L.					101:102	Allium sativum L.	86:102	Allium sativum L.	86:102	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.					
33095019	1	32	theme	anti-inflammation	235:251	arg1	properties					290:299	their anti-inflammation, antioxidation, and immunomodulation properties	229:299	their anti-inflammation, antioxidation, and immunomodulation properties	229:299	Garlic polysaccharides are great potential agents because of their anti-inflammation, antioxidation, and immunomodulation properties.					
33095019	0	33	theme	Modulation	44:53	arg1	Properties					55:64	Anti-Inflammatory and Intestinal Microbiota Modulation Properties	0:64	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.	0:166	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.					
33095019	0	34	theme	Sulfate-Induced	143:157	arg1	Colitis					159:165	Dextran Sodium Sulfate-Induced Colitis	128:165	Dextran Sodium Sulfate-Induced Colitis	128:165	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.					
33095019	6	35	theme	gut	1594:1596	arg1	microbiota					1598:1607	gut microbiota	1594:1607	gut microbiota	1594:1607	Taken together, this study proved that WSGP supplementation could alleviate DSS-induced colitis by improving mucosal barriers, blocking proinflammatory cytokines, and modulating gut microbiota.					
33095019	3	36	theme	Jinxiang	512:519	arg1	garlic					521:526	Jinxiang garlic	512:526	Jinxiang garlic	512:526	Herein, a water-soluble garlic polysaccharide (WSGP) was extracted from Jinxiang garlic to evaluate its effects on ameliorating dextran sulfate sodium (DSS)-induced colitis in a mouse model.					
33095019	0	37	theme	Jinxiang	69:76	arg1	Allium					86:91	Allium	86:91	Allium	86:91	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.					
33095019	0	37	theme	Jinxiang	69:76	arg1	Garlic					78:83	Jinxiang Garlic	69:83	Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis	69:165	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.					
33095019	4	38	theme	tissue	920:925	arg1	damage					927:932	the colonic tissue damage	908:932	the colonic tissue damage	908:932	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	39	theme	disease	805:811	arg1	index					822:826	the disease activity index	801:826	the disease activity index	801:826	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	3	40	theme	garlic	464:469	arg1	WSGP					487:490	WSGP	487:490	WSGP	487:490	Herein, a water-soluble garlic polysaccharide (WSGP) was extracted from Jinxiang garlic to evaluate its effects on ameliorating dextran sulfate sodium (DSS)-induced colitis in a mouse model.					
33095019	3	40	theme	garlic	464:469	arg1	polysaccharide					471:484	a water-soluble garlic polysaccharide	448:484	a water-soluble garlic polysaccharide (WSGP)	448:491	Herein, a water-soluble garlic polysaccharide (WSGP) was extracted from Jinxiang garlic to evaluate its effects on ameliorating dextran sulfate sodium (DSS)-induced colitis in a mouse model.					
33095019	4	41	theme	mice	866:869	arg1	score					849:853	the histological score	832:853	the histological score of colitic mice	832:869	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	41	theme	mice	866:869	arg1	index					822:826	the disease activity index	801:826	the disease activity index	801:826	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	42	theme	fatty	1114:1118	arg1	acids					1120:1124	short-chain fatty acids	1102:1124	short-chain fatty acids	1102:1124	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	2	43	theme	colon	386:390	arg1	system					392:397	the colon system	382:397	the colon system	382:397	However, few studies have reported their anti-inflammatory effects on improving the colon system and corresponding intestinal microbiota.					
33095019	3	44	from	colitis	605:611	arg1	model					624:628	a mouse model	616:628	a mouse model	616:628	Herein, a water-soluble garlic polysaccharide (WSGP) was extracted from Jinxiang garlic to evaluate its effects on ameliorating dextran sulfate sodium (DSS)-induced colitis in a mouse model.					
33095019	6	45	theme	DSS-induced	1492:1502	arg1	colitis					1504:1510	DSS-induced colitis	1492:1510	DSS-induced colitis	1492:1510	Taken together, this study proved that WSGP supplementation could alleviate DSS-induced colitis by improving mucosal barriers, blocking proinflammatory cytokines, and modulating gut microbiota.					
33095019	4	46	theme	mice	774:777	arg1	weight					738:743	body weight	733:743	body weight	733:743	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	46	theme	mice	774:777	arg1	length					756:761	colon length	750:761	colon length	750:761	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	46	theme	mice	774:777	arg1	intake					725:730	the feed intake	716:730	the feed intake	716:730	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	47	theme	short-chain	1102:1112	arg1	acids					1120:1124	short-chain fatty acids	1102:1124	short-chain fatty acids	1102:1124	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	6	48	theme	WSGP	1455:1458	arg1	supplementation					1460:1474	WSGP supplementation	1455:1474	WSGP supplementation	1455:1474	Taken together, this study proved that WSGP supplementation could alleviate DSS-induced colitis by improving mucosal barriers, blocking proinflammatory cytokines, and modulating gut microbiota.					
33095019	1	49	theme	antioxidation	254:266	arg1	properties					290:299	their anti-inflammation, antioxidation, and immunomodulation properties	229:299	their anti-inflammation, antioxidation, and immunomodulation properties	229:299	Garlic polysaccharides are great potential agents because of their anti-inflammation, antioxidation, and immunomodulation properties.					
33095019	4	50	theme	colitic	766:772	arg1	mice					774:777	colitic mice	766:777	colitic mice	766:777	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	51	theme	WSGP	1070:1073	arg1	WSGP					895:898	WSGP	895:898	the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha)	891:1055	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	1	52	theme	Garlic	168:173	arg1	agents					211:216	great potential agents	195:216	great potential agents	195:216	Garlic polysaccharides are great potential agents because of their anti-inflammation, antioxidation, and immunomodulation properties.					
33095019	1	52	theme	Garlic	168:173	arg1	polysaccharides					175:189	Garlic polysaccharides	168:189	Garlic polysaccharides	168:189	Garlic polysaccharides are great potential agents because of their anti-inflammation, antioxidation, and immunomodulation properties.					
33095019	4	53	theme	necrosis	1034:1041	arg1	alpha					1050:1054	tumor necrosis factor alpha	1028:1054	tumor necrosis factor alpha	1028:1054	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	53	theme	necrosis	1034:1041	arg1	factors					979:985	inflammatory factors	966:985	inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha)	966:1055	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	6	54	theme	proinflammatory	1552:1566	arg1	cytokines					1568:1576	proinflammatory cytokines	1552:1576	proinflammatory cytokines	1552:1576	Taken together, this study proved that WSGP supplementation could alleviate DSS-induced colitis by improving mucosal barriers, blocking proinflammatory cytokines, and modulating gut microbiota.					
33095019	4	55	theme	tumor	1028:1032	arg1	alpha					1050:1054	tumor necrosis factor alpha	1028:1054	tumor necrosis factor alpha	1028:1054	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	55	theme	tumor	1028:1032	arg1	factors					979:985	inflammatory factors	966:985	inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha)	966:1055	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	0	56	theme	Garlic	78:83	arg1	Polysaccharides					105:119	Jinxiang Garlic (Allium sativum L.) Polysaccharides	69:119	Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis	69:165	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.					
33095019	0	57	theme	Intestinal	22:31	arg1	Properties					55:64	Anti-Inflammatory and Intestinal Microbiota Modulation Properties	0:64	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.	0:166	Anti-Inflammatory and Intestinal Microbiota Modulation Properties of Jinxiang Garlic (Allium sativum L.) Polysaccharides toward Dextran Sodium Sulfate-Induced Colitis.					
33095019	2	58	theme	intestinal	417:426	arg1	microbiota					428:437	intestinal microbiota	417:437	intestinal microbiota	417:437	However, few studies have reported their anti-inflammatory effects on improving the colon system and corresponding intestinal microbiota.					
33095019	4	59	theme	interleukin	1003:1013	arg1	beta					1017:1020	interleukin 1 beta	1003:1020	interleukin 1 beta	1003:1020	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	4	59	theme	interleukin	1003:1013	arg1	factors					979:985	inflammatory factors	966:985	inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha)	966:1055	The results showed that (1) after administration of the WSGP (200 or 400 mg/kg/day), the feed intake, body weight, and colon length of colitic mice were increased, while the disease activity index and the histological score of colitic mice were decreased; (2) the WSGP reduced the colonic tissue damage and inhibited the expression of inflammatory factors (interleukin 6, interleukin 1 beta , and tumor necrosis factor alpha); and (3) the WSGP enhanced the production of short-chain fatty acids and improved the composition of intestinal microbiota.					
33095019	1	60	theme	immunomodulation	273:288	arg1	properties					290:299	their anti-inflammation, antioxidation, and immunomodulation properties	229:299	their anti-inflammation, antioxidation, and immunomodulation properties	229:299	Garlic polysaccharides are great potential agents because of their anti-inflammation, antioxidation, and immunomodulation properties.					
33055437	1	0	theme	brown	174:178	arg1	seaweed					180:186	Sargassum brown seaweed	164:186	Sargassum brown seaweed	164:186	Sargassum brown seaweed is known to have many health benefits and therapeutic effects.					
33055437	6	1	dep	variance	1337:1344	arg1	%					1352:1352	75.42%	1347:1352	75.42%	1347:1352	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	0	2	theme	Coastal	84:90	arg1	Areas					92:96	Different Coastal Areas	74:96	Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient	74:161	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	6	3	from	result	1032:1037	arg1	components					1056:1065	lipid-soluble components	1042:1065	lipid-soluble components	1042:1065	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	4	4	theme	different	729:737	arg1	origins					739:745	different origins	729:745	different origins	729:745	Principal component analysis (PCA) on water-soluble component made samples from different origins to be clearly distinguished (variance: 80.37%).					
33055437	5	5	contain	had	978:980	arg2	sugar					1005:1009	total sugar	999:1009	total sugar	999:1009	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	5	contain	had	978:980	arg2	amount					989:994	a high amount	982:994	a high amount of total sugar and crude fiber	982:1025	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	5	contain	had	978:980	arg1	samples					957:963	samples	957:963	samples of AB and PI	957:976	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	5	contain	had	978:980	arg2	fiber					1021:1025	crude fiber	1015:1025	crude fiber	1015:1025	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	1	6	contain	have	200:203	arg2	benefits					217:224	many health benefits	205:224	many health benefits	205:224	Sargassum brown seaweed is known to have many health benefits and therapeutic effects.					
33055437	1	6	contain	have	200:203	arg2	effects					242:248	therapeutic effects	230:248	therapeutic effects	230:248	Sargassum brown seaweed is known to have many health benefits and therapeutic effects.					
33055437	1	6	contain	have	200:203	arg1	seaweed					180:186	Sargassum brown seaweed	164:186	Sargassum brown seaweed	164:186	Sargassum brown seaweed is known to have many health benefits and therapeutic effects.					
33055437	6	7	theme	larger	1172:1177	arg1	size					1185:1188	larger blade size	1172:1188	larger blade size	1172:1188	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	8	theme	blade	1179:1183	arg1	size					1185:1188	larger blade size	1172:1188	larger blade size	1172:1188	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	7	9	theme	further	1441:1447	arg1	development					1449:1459	further development	1441:1459	further development	1441:1459	The overview of each samples' chemical characteristics can be valuable knowledge for further development, especially for developing functional ingredients.					
33055437	3	10	dep	differences	431:441	arg1	both					444:447	both	444:447	both	444:447	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	3	11	theme	coastal	530:536	arg1	areas					538:542	four different coastal areas	515:542	four different coastal areas	515:542	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	3	11	theme	coastal	530:536	arg1	Bay/AB					586:591	Bay/AB	586:591	Bay/AB	586:591	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	3	11	theme	coastal	530:536	arg1	Beach/SHB					638:646	Beach/SHB	638:646	Beach/SHB	638:646	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	3	11	theme	coastal	530:536	arg1	Beach/UGB					608:616	Beach/UGB	608:616	Beach/UGB	608:616	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	6	12	theme	close	1135:1139	arg1	proximity					1141:1149	close proximity	1135:1149	close proximity	1135:1149	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	5	13	theme	AB	968:969	arg1	samples					957:963	samples	957:963	samples of AB and PI	957:976	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	3	14	theme	lipidsoluble	459:470	arg1	component					472:480	lipidsoluble component	459:480	lipidsoluble component	459:480	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	4	15	theme	water-soluble	687:699	arg1	component					701:709	water-soluble component	687:709	water-soluble component	687:709	Principal component analysis (PCA) on water-soluble component made samples from different origins to be clearly distinguished (variance: 80.37%).					
33055437	6	16	theme	fatty	1286:1290	arg1	acids					1292:1296	total n-6 fatty acids	1276:1296	total n-6 fatty acids	1276:1296	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	17	theme	lipid-soluble	1042:1054	arg1	components					1056:1065	lipid-soluble components	1042:1065	lipid-soluble components	1042:1065	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	2	18	theme	seaweed	297:303	arg1	characterization					272:287	Preliminary chemical characterization	251:287	Preliminary chemical characterization of this seaweed	251:303	Preliminary chemical characterization of this seaweed is important as a bioprospecting strategy for seaweed industry development.					
33055437	3	19	theme	composition	419:429	arg1	differences					431:441	chemical composition differences	410:441	chemical composition differences	410:441	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	5	20	theme	SHB	795:797	arg1	samples					807:813	SHB and UGB samples	795:813	samples	807:813	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	6	21	theme	chlorophyll	1209:1219	arg1	fucoxanthin					1222:1232	fucoxanthin	1222:1232	fucoxanthin	1222:1232	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	21	theme	chlorophyll	1209:1219	arg1	size					1185:1188	larger blade size	1172:1188	larger blade size	1172:1188	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	21	theme	chlorophyll	1209:1219	arg1	ratio					1316:1320	a lower ratio	1308:1320	a lower ratio of n-6 to n-3 (variance: 75.42%)	1308:1353	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	21	theme	chlorophyll	1209:1219	arg1	acids					1292:1296	total n-6 fatty acids	1276:1296	total n-6 fatty acids	1276:1296	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	21	theme	chlorophyll	1209:1219	arg1	content					1198:1204	higher content	1191:1204	higher content of chlorophyll	1191:1219	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	21	theme	chlorophyll	1209:1219	arg1	carotenoid					1235:1244	carotenoid	1235:1244	carotenoid	1235:1244	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	21	theme	chlorophyll	1209:1219	arg1	acids					1269:1273	total n-3 fatty acids	1253:1273	total n-3 fatty acids	1253:1273	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	21	theme	chlorophyll	1209:1219	arg1	PUFA					1247:1250	PUFA	1247:1250	PUFA	1247:1250	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	4	22	dep	distinguished	761:773	arg1	variance					776:783	variance	776:783	variance: 80.37%	776:791	Principal component analysis (PCA) on water-soluble component made samples from different origins to be clearly distinguished (variance: 80.37%).					
33055437	6	23	from	proximity	1141:1149	arg1	located					1124:1130	located	1124:1130	located	1124:1130	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	3	24	theme	chemical	410:417	arg1	differences					431:441	chemical composition differences	410:441	chemical composition differences	410:441	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	0	25	with	Indonesia	101:109	arg1	Potential					116:124	Potential	116:124	Potential	116:124	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	5	26	theme	FCSPs	944:948	arg1	ash					855:857	ash	855:857	ash	855:857	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	26	theme	FCSPs	944:948	arg1	FCSPs					914:918	FCSPs	914:918	FCSPs	914:918	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	26	theme	FCSPs	944:948	arg1	polysaccharides					897:911	fucose-containing sulfated polysaccharides	870:911	fucose-containing sulfated polysaccharides (FCSPs)	870:919	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	26	theme	FCSPs	944:948	arg1	alginate					860:867	alginate	860:867	alginate	860:867	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	26	theme	FCSPs	944:948	arg1	content					933:939	fucose content	926:939	fucose content of FCSPs	926:948	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	27	theme	sulfated	888:895	arg1	FCSPs					914:918	FCSPs	914:918	FCSPs	914:918	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	27	theme	sulfated	888:895	arg1	polysaccharides					897:911	fucose-containing sulfated polysaccharides	870:911	fucose-containing sulfated polysaccharides (FCSPs)	870:919	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	0	28	theme	Component	24:32	arg1	Profile					34:40	Lipid-Soluble Component Profile	10:40	Lipid-Soluble Component Profile	10:40	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	3	29	from	areas	538:542	arg1	water					449:453	water	449:453	water	449:453	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	3	29	from	areas	538:542	arg1	component					472:480	lipidsoluble component	459:480	lipidsoluble component	459:480	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	3	29	from	areas	538:542	arg1	Indonesia					547:555	Indonesia	547:555	Indonesia	547:555	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	6	30	theme	n-6	1282:1284	arg1	acids					1292:1296	total n-6 fatty acids	1276:1296	total n-6 fatty acids	1276:1296	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	5	31	theme	content	933:939	arg1	content					844:850	a high content	837:850	a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs	837:948	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	2	32	theme	bioprospecting	323:336	arg1	strategy					338:345	a bioprospecting strategy	321:345	a bioprospecting strategy for seaweed industry development	321:378	Preliminary chemical characterization of this seaweed is important as a bioprospecting strategy for seaweed industry development.					
33055437	6	33	dep	showed	1067:1072	arg1	characterized					1155:1167	characterized	1155:1167	characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%)	1155:1353	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	33	dep	showed	1067:1072	arg1	located					1124:1130	located	1124:1130	located	1124:1130	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	1	34	theme	many	205:208	arg1	benefits					217:224	many health benefits	205:224	many health benefits	205:224	Sargassum brown seaweed is known to have many health benefits and therapeutic effects.					
33055437	0	35	theme	Lipid-Soluble	10:22	arg1	Profile					34:40	Lipid-Soluble Component Profile	10:40	Lipid-Soluble Component Profile	10:40	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	6	36	theme	total	1276:1280	arg1	acids					1292:1296	total n-6 fatty acids	1276:1296	total n-6 fatty acids	1276:1296	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	0	37	from	Areas	92:96	arg1	Indonesia					101:109	Indonesia	101:109	Indonesia with Potential for Developing Functional Ingredient	101:161	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	0	37	from	Areas	92:96	arg1	Water					0:4	Water	0:4	Water	0:4	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	0	37	from	Areas	92:96	arg1	Profile					34:40	Lipid-Soluble Component Profile	10:40	Lipid-Soluble Component Profile	10:40	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	6	38	theme	AB	1108:1109	arg1	samples					1111:1117	AB samples	1108:1117	AB samples	1108:1117	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	7	39	theme	functional	1488:1497	arg1	ingredients					1499:1509	functional ingredients	1488:1509	functional ingredients	1488:1509	The overview of each samples' chemical characteristics can be valuable knowledge for further development, especially for developing functional ingredients.					
33055437	1	40	theme	health	210:215	arg1	benefits					217:224	many health benefits	205:224	many health benefits	205:224	Sargassum brown seaweed is known to have many health benefits and therapeutic effects.					
33055437	6	41	theme	n-3	1332:1334	arg1	fucoxanthin					1222:1232	fucoxanthin	1222:1232	fucoxanthin	1222:1232	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	41	theme	n-3	1332:1334	arg1	size					1185:1188	larger blade size	1172:1188	larger blade size	1172:1188	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	41	theme	n-3	1332:1334	arg1	ratio					1316:1320	a lower ratio	1308:1320	a lower ratio of n-6 to n-3 (variance: 75.42%)	1308:1353	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	41	theme	n-3	1332:1334	arg1	acids					1292:1296	total n-6 fatty acids	1276:1296	total n-6 fatty acids	1276:1296	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	41	theme	n-3	1332:1334	arg1	content					1198:1204	higher content	1191:1204	higher content of chlorophyll	1191:1219	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	41	theme	n-3	1332:1334	arg1	carotenoid					1235:1244	carotenoid	1235:1244	carotenoid	1235:1244	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	41	theme	n-3	1332:1334	arg1	acids					1269:1273	total n-3 fatty acids	1253:1273	total n-3 fatty acids	1253:1273	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	41	theme	n-3	1332:1334	arg1	PUFA					1247:1250	PUFA	1247:1250	PUFA	1247:1250	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	6	42	dep	n-3	1332:1334	arg1	to					1329:1330	to	1329:1330	to	1329:1330	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	2	43	theme	Preliminary	251:261	arg1	characterization					272:287	Preliminary chemical characterization	251:287	Preliminary chemical characterization of this seaweed	251:303	Preliminary chemical characterization of this seaweed is important as a bioprospecting strategy for seaweed industry development.					
33055437	4	44	from	analysis	669:676	arg1	component					701:709	water-soluble component	687:709	water-soluble component	687:709	Principal component analysis (PCA) on water-soluble component made samples from different origins to be clearly distinguished (variance: 80.37%).					
33055437	5	45	theme	ash	855:857	arg1	content					844:850	a high content	837:850	a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs	837:948	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	46	theme	UGB	803:805	arg1	samples					807:813	SHB and UGB samples	795:813	samples	807:813	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	0	47	theme	Functional	141:150	arg1	Ingredient					152:161	Functional Ingredient	141:161	Functional Ingredient	141:161	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	6	48	theme	higher	1191:1196	arg1	content					1198:1204	higher content	1191:1204	higher content of chlorophyll	1191:1219	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	3	49	theme	cristaefolium	496:508	arg1	water					449:453	water	449:453	water	449:453	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	3	49	theme	cristaefolium	496:508	arg1	component					472:480	lipidsoluble component	459:480	lipidsoluble component	459:480	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	0	50	theme	cristaefolium	55:67	arg1	Water					0:4	Water	0:4	Water	0:4	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	0	50	theme	cristaefolium	55:67	arg1	Profile					34:40	Lipid-Soluble Component Profile	10:40	Lipid-Soluble Component Profile	10:40	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	5	51	theme	fucose-containing	870:886	arg1	FCSPs					914:918	FCSPs	914:918	FCSPs	914:918	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	51	theme	fucose-containing	870:886	arg1	polysaccharides					897:911	fucose-containing sulfated polysaccharides	870:911	fucose-containing sulfated polysaccharides (FCSPs)	870:919	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	6	52	theme	fatty	1263:1267	arg1	acids					1269:1273	total n-3 fatty acids	1253:1273	total n-3 fatty acids	1253:1273	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	7	53	theme	valuable	1418:1425	arg1	knowledge					1427:1435	valuable knowledge	1418:1435	valuable knowledge for further development, especially for developing functional ingredients	1418:1509	The overview of each samples' chemical characteristics can be valuable knowledge for further development, especially for developing functional ingredients.					
33055437	7	53	theme	valuable	1418:1425	arg1	overview					1360:1367	The overview	1356:1367	The overview of each samples' chemical characteristics	1356:1409	The overview of each samples' chemical characteristics can be valuable knowledge for further development, especially for developing functional ingredients.					
33055437	5	54	theme	total	999:1003	arg1	sugar					1005:1009	total sugar	999:1009	total sugar	999:1009	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	6	55	theme	different	1076:1084	arg1	tendency					1086:1093	a different tendency	1074:1093	a different tendency that SHB and AB samples	1074:1117	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	3	56	dep	Bay/AB	586:591	arg1	Awur					581:584	Awur	581:584	Awur	581:584	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	6	57	theme	n-3	1259:1261	arg1	acids					1269:1273	total n-3 fatty acids	1253:1273	total n-3 fatty acids	1253:1273	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	7	58	theme	characteristics	1395:1409	arg1	knowledge					1427:1435	valuable knowledge	1418:1435	valuable knowledge for further development, especially for developing functional ingredients	1418:1509	The overview of each samples' chemical characteristics can be valuable knowledge for further development, especially for developing functional ingredients.					
33055437	7	58	theme	characteristics	1395:1409	arg1	overview					1360:1367	The overview	1356:1367	The overview of each samples' chemical characteristics	1356:1409	The overview of each samples' chemical characteristics can be valuable knowledge for further development, especially for developing functional ingredients.					
33055437	5	59	theme	sugar	1005:1009	arg1	fiber					1021:1025	crude fiber	1015:1025	crude fiber	1015:1025	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	59	theme	sugar	1005:1009	arg1	amount					989:994	a high amount	982:994	a high amount of total sugar and crude fiber	982:1025	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	59	theme	sugar	1005:1009	arg1	sugar					1005:1009	total sugar	999:1009	total sugar	999:1009	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	4	60	dep	variance	776:783	arg1	%					791:791	80.37%	786:791	variance: 80.37%	776:791	Principal component analysis (PCA) on water-soluble component made samples from different origins to be clearly distinguished (variance: 80.37%).					
33055437	5	61	theme	high	984:987	arg1	fiber					1021:1025	crude fiber	1015:1025	crude fiber	1015:1025	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	61	theme	high	984:987	arg1	amount					989:994	a high amount	982:994	a high amount of total sugar and crude fiber	982:1025	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	61	theme	high	984:987	arg1	sugar					1005:1009	total sugar	999:1009	total sugar	999:1009	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	6	62	theme	total	1253:1257	arg1	acids					1269:1273	total n-3 fatty acids	1253:1273	total n-3 fatty acids	1253:1273	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	5	63	theme	crude	1015:1019	arg1	fiber					1021:1025	crude fiber	1015:1025	crude fiber	1015:1025	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	64	theme	PI	975:976	arg1	samples					957:963	samples	957:963	samples of AB and PI	957:976	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	2	65	theme	industry	359:366	arg1	development					368:378	seaweed industry development	351:378	seaweed industry development	351:378	Preliminary chemical characterization of this seaweed is important as a bioprospecting strategy for seaweed industry development.					
33055437	1	66	theme	therapeutic	230:240	arg1	effects					242:248	therapeutic effects	230:248	therapeutic effects	230:248	Sargassum brown seaweed is known to have many health benefits and therapeutic effects.					
33055437	5	67	theme	fucose	926:931	arg1	content					933:939	fucose content	926:939	fucose content of FCSPs	926:948	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	68	theme	fiber	1021:1025	arg1	fiber					1021:1025	crude fiber	1015:1025	crude fiber	1015:1025	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	68	theme	fiber	1021:1025	arg1	amount					989:994	a high amount	982:994	a high amount of total sugar and crude fiber	982:1025	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	68	theme	fiber	1021:1025	arg1	sugar					1005:1009	total sugar	999:1009	total sugar	999:1009	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	69	theme	high	839:842	arg1	content					844:850	a high content	837:850	a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs	837:948	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	5	70	theme	alginate	860:867	arg1	content					844:850	a high content	837:850	a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs	837:948	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	2	71	theme	seaweed	351:357	arg1	development					368:378	seaweed industry development	351:378	seaweed industry development	351:378	Preliminary chemical characterization of this seaweed is important as a bioprospecting strategy for seaweed industry development.					
33055437	3	72	theme	different	520:528	arg1	areas					538:542	four different coastal areas	515:542	four different coastal areas	515:542	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	3	72	theme	different	520:528	arg1	Bay/AB					586:591	Bay/AB	586:591	Bay/AB	586:591	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	3	72	theme	different	520:528	arg1	Beach/SHB					638:646	Beach/SHB	638:646	Beach/SHB	638:646	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	3	72	theme	different	520:528	arg1	Beach/UGB					608:616	Beach/UGB	608:616	Beach/UGB	608:616	This study aimed to evaluate chemical composition differences, both water and lipidsoluble component, of Sargassum cristaefolium from four different coastal areas in Indonesia, namely Pari Island/PI, Awur Bay/AB, Ujung Genteng Beach/UGB, and Sayang Heulang Beach/SHB.					
33055437	0	73	theme	Different	74:82	arg1	Areas					92:96	Different Coastal Areas	74:96	Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient	74:161	Water and Lipid-Soluble Component Profile of Sargassum cristaefolium from Different Coastal Areas in Indonesia with Potential for Developing Functional Ingredient.					
33055437	7	74	theme	chemical	1386:1393	arg1	characteristics					1395:1409	each samples' chemical characteristics	1372:1409	each samples' chemical characteristics	1372:1409	The overview of each samples' chemical characteristics can be valuable knowledge for further development, especially for developing functional ingredients.					
33055437	5	75	theme	polysaccharides	897:911	arg1	content					844:850	a high content	837:850	a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs	837:948	SHB and UGB samples were characterized by a high content of ash, alginate, fucose-containing sulfated polysaccharides (FCSPs), and fucose content of FCSPs, while samples of AB and PI had a high amount of total sugar and crude fiber.					
33055437	6	76	theme	PCA	1028:1030	arg1	result					1032:1037	PCA result	1028:1037	PCA result on lipid-soluble components	1028:1065	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	4	77	theme	component	659:667	arg1	PCA					679:681	PCA	679:681	PCA	679:681	Principal component analysis (PCA) on water-soluble component made samples from different origins to be clearly distinguished (variance: 80.37%).					
33055437	4	77	theme	component	659:667	arg1	analysis					669:676	Principal component analysis	649:676	Principal component analysis (PCA) on water-soluble component	649:709	Principal component analysis (PCA) on water-soluble component made samples from different origins to be clearly distinguished (variance: 80.37%).					
33055437	6	78	theme	lower	1310:1314	arg1	ratio					1316:1320	a lower ratio	1308:1320	a lower ratio of n-6 to n-3 (variance: 75.42%)	1308:1353	PCA result on lipid-soluble components showed a different tendency that SHB and AB samples were located at close proximity and characterized by larger blade size, higher content of chlorophyll, fucoxanthin, carotenoid, PUFA, total n-3 fatty acids, total n-6 fatty acids, and also a lower ratio of n-6 to n-3 (variance: 75.42%).					
33055437	2	79	theme	chemical	263:270	arg1	characterization					272:287	Preliminary chemical characterization	251:287	Preliminary chemical characterization of this seaweed	251:303	Preliminary chemical characterization of this seaweed is important as a bioprospecting strategy for seaweed industry development.					
33055437	1	80	theme	Sargassum	164:172	arg1	seaweed					180:186	Sargassum brown seaweed	164:186	Sargassum brown seaweed	164:186	Sargassum brown seaweed is known to have many health benefits and therapeutic effects.					
33055437	4	81	from	origins	739:745	arg1	samples					716:722	samples	716:722	samples from different origins	716:745	Principal component analysis (PCA) on water-soluble component made samples from different origins to be clearly distinguished (variance: 80.37%).					
33055437	4	82	theme	Principal	649:657	arg1	PCA					679:681	PCA	679:681	PCA	679:681	Principal component analysis (PCA) on water-soluble component made samples from different origins to be clearly distinguished (variance: 80.37%).					
33055437	4	82	theme	Principal	649:657	arg1	analysis					669:676	Principal component analysis	649:676	Principal component analysis (PCA) on water-soluble component	649:709	Principal component analysis (PCA) on water-soluble component made samples from different origins to be clearly distinguished (variance: 80.37%).					
32600693	15	0	theme	highest	2529:2535	arg1	concentration					2537:2549	the highest concentration	2525:2549	the highest concentration	2525:2549	However, the PEG and PEG-MC containing colloids proved to be relatively non-toxic, even at the highest concentration, but with a slowly decreasing tendency of the cell metabolic activity.					
32600693	13	1	from	0.6 μg/ml	2173:2181	arg1	range					2162:2166	the range	2158:2166	the range from 0.6 μg/ml to 6 μg/ml	2158:2192	The studied colloid concentrations were in the range from 0.6 μg/ml to 6 μg/ml.					
32600693	10	2	theme	drip	1630:1633	arg1	test					1635:1638	a drip test	1628:1638	a drip test	1628:1638	Antimicrobial tests were performed by a drip test on two bacterial strains - Gram-positive Staphylococcus epidermidis and Gram-negative Escherichia coli.					
32600693	12	3	from	dose	2078:2081	arg1	cells					2108:2112	human cells	2102:2112	human cells	2102:2112	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	14	4	from	chitosan	2289:2296	arg1	colloid					2305:2311	the colloid	2301:2311	the colloid	2301:2311	Toxicity of the colloids started to reappear at a concentration of 4.5 μg/ml, especially with chitosan in the colloid, where the colloid with a concentration of 6 μg/ml proved to be the most toxic, especially towards the SAOS-2 cell line.					
32600693	5	5	theme	light	828:832	arg1	scattering					834:843	dynamic light scattering	820:843	dynamic light scattering	820:843	The colloids were sterilized by steam, and their stability was measured before and after the sterilization process by dynamic light scattering and UV-Vis spectrophotometry.					
32600693	12	6	theme	primary	1951:1957	arg1	NHDF					1992:1995	NHDF	1992:1995	NHDF	1992:1995	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	6	theme	primary	1951:1957	arg1	fibroblasts					1979:1989	primary normal human dermal fibroblasts	1951:1989	primary normal human dermal fibroblasts (NHDF)	1951:1996	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	9	7	theme	colloids	1580:1587	arg1	activity					1539:1546	the biological activity	1524:1546	the biological activity	1524:1546	In addition, in vitro tests of antimicrobial activity and cytotoxicity were carried out to estimate the biological activity and the biocompatibility of the colloids.					
32600693	9	7	theme	colloids	1580:1587	arg1	biocompatibility					1556:1571	the biocompatibility	1552:1571	the biocompatibility of the colloids	1552:1587	In addition, in vitro tests of antimicrobial activity and cytotoxicity were carried out to estimate the biological activity and the biocompatibility of the colloids.					
32600693	7	8	theme	absorption	1018:1027	arg1	AAS					1043:1045	AAS	1043:1045	AAS	1043:1045	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	7	8	theme	absorption	1018:1027	arg1	spectroscopy					1029:1040	atomic absorption spectroscopy	1011:1040	atomic absorption spectroscopy (AAS)	1011:1046	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	10	9	theme	bacterial	1647:1655	arg1	strains					1657:1663	two bacterial strains	1643:1663	two bacterial strains - Gram-positive Staphylococcus epidermidis and Gram-negative Escherichia coli	1643:1741	Antimicrobial tests were performed by a drip test on two bacterial strains - Gram-positive Staphylococcus epidermidis and Gram-negative Escherichia coli.					
32600693	1	10	theme	effective	147:155	arg1	AuNPs					187:191	AuNPs	187:191	AuNPs	187:191	We have developed a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine.					
32600693	1	10	theme	effective	147:155	arg1	nanoparticles					172:184	effective preparing gold nanoparticles	147:184	effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine	147:232	We have developed a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine.					
32600693	12	11	theme	human	1911:1915	arg1	SAOS-2					1940:1945	human osteoblastic cell line SAOS-2	1911:1945	human osteoblastic cell line SAOS-2	1911:1945	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	14	12	theme	4.5 μg/ml	2262:2270	arg1	concentration					2245:2257	a concentration	2243:2257	a concentration of 4.5 μg/ml	2243:2270	Toxicity of the colloids started to reappear at a concentration of 4.5 μg/ml, especially with chitosan in the colloid, where the colloid with a concentration of 6 μg/ml proved to be the most toxic, especially towards the SAOS-2 cell line.					
32600693	2	13	theme	liquid	279:284	arg1	glycol					300:305	ethylene glycol	291:305	ethylene glycol	291:305	The method is based on gold sputtering into liquid poly(ethylene glycol) (PEG).					
32600693	2	13	theme	liquid	279:284	arg1	PEG					309:311	PEG	309:311	PEG	309:311	The method is based on gold sputtering into liquid poly(ethylene glycol) (PEG).					
32600693	2	13	theme	liquid	279:284	arg1	poly					286:289	liquid poly	279:289	liquid poly(ethylene glycol) (PEG)	279:312	The method is based on gold sputtering into liquid poly(ethylene glycol) (PEG).					
32600693	1	14	theme	gold	167:170	arg1	AuNPs					187:191	AuNPs	187:191	AuNPs	187:191	We have developed a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine.					
32600693	1	14	theme	gold	167:170	arg1	nanoparticles					172:184	effective preparing gold nanoparticles	147:184	effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine	147:232	We have developed a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine.					
32600693	12	15	theme	cell	1930:1933	arg1	SAOS-2					1940:1945	human osteoblastic cell line SAOS-2	1911:1945	human osteoblastic cell line SAOS-2	1911:1945	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	16	theme	eukaryotic	1888:1897	arg1	cells					1899:1903	eukaryotic cells	1888:1903	eukaryotic cells	1888:1903	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	8	17	theme	important	1338:1346	arg1	parameter					1348:1356	an important parameter	1335:1356	an important parameter indicating the stability and the biocompatibility of the colloid	1335:1421	The zeta-potential of the AuNPs was also determined as an important parameter indicating the stability and the biocompatibility of the colloid.					
32600693	8	17	theme	important	1338:1346	arg1	zeta-potential					1284:1297	The zeta-potential	1280:1297	The zeta-potential of the AuNPs	1280:1310	The zeta-potential of the AuNPs was also determined as an important parameter indicating the stability and the biocompatibility of the colloid.					
32600693	4	18	with	base	522:525	arg1	aims					536:539	the aims	532:539	the aims	532:539	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	7	19	theme	colloids	1270:1277	arg1	viscosity					1253:1261	the viscosity	1249:1261	the viscosity of the colloids	1249:1277	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	5	20	theme	dynamic	820:826	arg1	scattering					834:843	dynamic light scattering	820:843	dynamic light scattering	820:843	The colloids were sterilized by steam, and their stability was measured before and after the sterilization process by dynamic light scattering and UV-Vis spectrophotometry.					
32600693	10	21	dep	strains	1657:1663	arg1	epidermidis					1696:1706	Gram-positive Staphylococcus epidermidis	1667:1706	Gram-positive Staphylococcus epidermidis	1667:1706	Antimicrobial tests were performed by a drip test on two bacterial strains - Gram-positive Staphylococcus epidermidis and Gram-negative Escherichia coli.					
32600693	10	21	dep	strains	1657:1663	arg1	coli					1738:1741	Gram-negative Escherichia coli	1712:1741	Gram-negative Escherichia coli	1712:1741	Antimicrobial tests were performed by a drip test on two bacterial strains - Gram-positive Staphylococcus epidermidis and Gram-negative Escherichia coli.					
32600693	1	22	theme	novel	123:127	arg1	method					136:141	a novel simple method	121:141	a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine	121:232	We have developed a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine.					
32600693	9	23	theme	in	1437:1438	arg1	tests					1446:1450	in vitro tests	1437:1450	in vitro tests of antimicrobial activity and cytotoxicity	1437:1493	In addition, in vitro tests of antimicrobial activity and cytotoxicity were carried out to estimate the biological activity and the biocompatibility of the colloids.					
32600693	6	24	theme	higher	899:904	arg1	yield					917:921	a higher sputtering yield	897:921	a higher sputtering yield in the colloids containing the polysaccharides	897:968	The results indicated a higher sputtering yield in the colloids containing the polysaccharides.					
32600693	8	25	theme	colloid	1415:1421	arg1	stability					1373:1381	the stability	1369:1381	the stability	1369:1381	The zeta-potential of the AuNPs was also determined as an important parameter indicating the stability and the biocompatibility of the colloid.					
32600693	8	25	theme	colloid	1415:1421	arg1	biocompatibility					1391:1406	the biocompatibility	1387:1406	the biocompatibility of the colloid	1387:1421	The zeta-potential of the AuNPs was also determined as an important parameter indicating the stability and the biocompatibility of the colloid.					
32600693	12	26	from	effect	2057:2062	arg1	cells					2108:2112	human cells	2102:2112	human cells	2102:2112	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	15	27	theme	activity	2612:2619	arg1	tendency					2581:2588	a slowly decreasing tendency	2561:2588	a slowly decreasing tendency of the cell metabolic activity	2561:2619	However, the PEG and PEG-MC containing colloids proved to be relatively non-toxic, even at the highest concentration, but with a slowly decreasing tendency of the cell metabolic activity.					
32600693	0	28	theme	other	85:89	arg1	sputtering					43:52	sputtering	43:52	sputtering	43:52	A new way to prepare gold nanoparticles by sputtering - Sterilization, stability and other properties.					
32600693	0	28	theme	other	85:89	arg1	properties					91:100	other properties	85:100	other properties	85:100	A new way to prepare gold nanoparticles by sputtering - Sterilization, stability and other properties.					
32600693	9	29	theme	antimicrobial	1455:1467	arg1	activity					1469:1476	antimicrobial activity	1455:1476	antimicrobial activity	1455:1476	In addition, in vitro tests of antimicrobial activity and cytotoxicity were carried out to estimate the biological activity and the biocompatibility of the colloids.					
32600693	7	30	theme	nanoparticles	1090:1102	arg1	composition					1062:1072	the composition	1058:1072	the composition of the prepared nanoparticles by transmission electron microscopy (TEM)	1058:1144	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	15	31	theme	metabolic	2602:2610	arg1	activity					2612:2619	the cell metabolic activity	2593:2619	the cell metabolic activity	2593:2619	However, the PEG and PEG-MC containing colloids proved to be relatively non-toxic, even at the highest concentration, but with a slowly decreasing tendency of the cell metabolic activity.					
32600693	5	32	theme	UV-Vis	849:854	arg1	spectrophotometry					856:872	UV-Vis spectrophotometry	849:872	UV-Vis spectrophotometry	849:872	The colloids were sterilized by steam, and their stability was measured before and after the sterilization process by dynamic light scattering and UV-Vis spectrophotometry.					
32600693	3	33	theme	basic	337:341	arg1	stabilizer					357:366	a basic biocompatible stabilizer	335:366	a basic biocompatible stabilizer of the AuNP colloid	335:386	The PEG was used as a basic biocompatible stabilizer of the AuNP colloid.					
32600693	3	33	theme	basic	337:341	arg1	PEG					319:321	The PEG	315:321	The PEG	315:321	The PEG was used as a basic biocompatible stabilizer of the AuNP colloid.					
32600693	12	34	dep	SAOS-2	1940:1945	arg1	i.e.					1906:1909	i.e.	1906:1909	i.e.	1906:1909	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	3	35	theme	colloid	380:386	arg1	stabilizer					357:366	a basic biocompatible stabilizer	335:366	a basic biocompatible stabilizer of the AuNP colloid	335:386	The PEG was used as a basic biocompatible stabilizer of the AuNP colloid.					
32600693	3	35	theme	colloid	380:386	arg1	PEG					319:321	The PEG	315:321	The PEG	315:321	The PEG was used as a basic biocompatible stabilizer of the AuNP colloid.					
32600693	14	36	theme	cell	2423:2426	arg1	line					2428:2431	the SAOS-2 cell line	2412:2431	the SAOS-2 cell line	2412:2431	Toxicity of the colloids started to reappear at a concentration of 4.5 μg/ml, especially with chitosan in the colloid, where the colloid with a concentration of 6 μg/ml proved to be the most toxic, especially towards the SAOS-2 cell line.					
32600693	11	37	theme	highest	1785:1791	arg1	activity					1807:1814	the highest antibacterial activity	1781:1814	the highest antibacterial activity	1781:1814	AuNP with chitosan proved to possess the highest antibacterial activity, especially towards the Gram-positive S. epidermidis.					
32600693	13	38	theme	studied	2119:2125	arg1	concentrations					2135:2148	The studied colloid concentrations	2115:2148	The studied colloid concentrations	2115:2148	The studied colloid concentrations were in the range from 0.6 μg/ml to 6 μg/ml.					
32600693	11	39	contain	possess	1773:1779	arg1	AuNP					1744:1747	AuNP	1744:1747	AuNP with chitosan	1744:1761	AuNP with chitosan proved to possess the highest antibacterial activity, especially towards the Gram-positive S. epidermidis.					
32600693	11	39	contain	possess	1773:1779	arg2	activity					1807:1814	the highest antibacterial activity	1781:1814	the highest antibacterial activity	1781:1814	AuNP with chitosan proved to possess the highest antibacterial activity, especially towards the Gram-positive S. epidermidis.					
32600693	2	40	theme	ethylene	291:298	arg1	glycol					300:305	ethylene glycol	291:305	ethylene glycol	291:305	The method is based on gold sputtering into liquid poly(ethylene glycol) (PEG).					
32600693	2	40	theme	ethylene	291:298	arg1	poly					286:289	liquid poly	279:289	liquid poly(ethylene glycol) (PEG)	279:312	The method is based on gold sputtering into liquid poly(ethylene glycol) (PEG).					
32600693	9	41	theme	biological	1528:1537	arg1	activity					1539:1546	the biological activity	1524:1546	the biological activity	1524:1546	In addition, in vitro tests of antimicrobial activity and cytotoxicity were carried out to estimate the biological activity and the biocompatibility of the colloids.					
32600693	12	42	from	colloids	2090:2097	arg1	cells					2108:2112	human cells	2102:2112	human cells	2102:2112	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	43	theme	normal	1959:1964	arg1	NHDF					1992:1995	NHDF	1992:1995	NHDF	1992:1995	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	43	theme	normal	1959:1964	arg1	fibroblasts					1979:1989	primary normal human dermal fibroblasts	1951:1989	primary normal human dermal fibroblasts (NHDF)	1951:1996	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	44	theme	dermal	1972:1977	arg1	NHDF					1992:1995	NHDF	1992:1995	NHDF	1992:1995	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	44	theme	dermal	1972:1977	arg1	fibroblasts					1979:1989	primary normal human dermal fibroblasts	1951:1989	primary normal human dermal fibroblasts (NHDF)	1951:1996	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	2	45	theme	gold	258:261	arg1	sputtering					263:272	gold sputtering	258:272	gold sputtering into liquid poly(ethylene glycol) (PEG)	258:312	The method is based on gold sputtering into liquid poly(ethylene glycol) (PEG).					
32600693	12	46	theme	line	1935:1938	arg1	SAOS-2					1940:1945	human osteoblastic cell line SAOS-2	1911:1945	human osteoblastic cell line SAOS-2	1911:1945	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	7	47	theme	atomic	1011:1016	arg1	AAS					1043:1045	AAS	1043:1045	AAS	1043:1045	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	7	47	theme	atomic	1011:1016	arg1	spectroscopy					1029:1040	atomic absorption spectroscopy	1011:1040	atomic absorption spectroscopy (AAS)	1011:1046	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	14	48	theme	6 μg/ml	2356:2362	arg1	concentration					2339:2351	a concentration	2337:2351	a concentration of 6 μg/ml	2337:2362	Toxicity of the colloids started to reappear at a concentration of 4.5 μg/ml, especially with chitosan in the colloid, where the colloid with a concentration of 6 μg/ml proved to be the most toxic, especially towards the SAOS-2 cell line.					
32600693	1	49	theme	preparing	157:165	arg1	AuNPs					187:191	AuNPs	187:191	AuNPs	187:191	We have developed a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine.					
32600693	1	49	theme	preparing	157:165	arg1	nanoparticles					172:184	effective preparing gold nanoparticles	147:184	effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine	147:232	We have developed a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine.					
32600693	14	50	with	colloid	2324:2330	arg1	concentration					2339:2351	a concentration	2337:2351	a concentration of 6 μg/ml	2337:2362	Toxicity of the colloids started to reappear at a concentration of 4.5 μg/ml, especially with chitosan in the colloid, where the colloid with a concentration of 6 μg/ml proved to be the most toxic, especially towards the SAOS-2 cell line.					
32600693	8	51	theme	AuNPs	1306:1310	arg1	parameter					1348:1356	an important parameter	1335:1356	an important parameter indicating the stability and the biocompatibility of the colloid	1335:1421	The zeta-potential of the AuNPs was also determined as an important parameter indicating the stability and the biocompatibility of the colloid.					
32600693	8	51	theme	AuNPs	1306:1310	arg1	zeta-potential					1284:1297	The zeta-potential	1280:1297	The zeta-potential of the AuNPs	1280:1310	The zeta-potential of the AuNPs was also determined as an important parameter indicating the stability and the biocompatibility of the colloid.					
32600693	12	52	theme	osteoblastic	1917:1928	arg1	SAOS-2					1940:1945	human osteoblastic cell line SAOS-2	1911:1945	human osteoblastic cell line SAOS-2	1911:1945	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	53	theme	7-day	2022:2026	arg1	cultivation					2028:2038	a 7-day cultivation	2020:2038	a 7-day cultivation	2020:2038	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	15	54	theme	containing	2462:2471	arg1	colloids					2473:2480	the PEG and PEG-MC containing colloids	2443:2480	colloids	2473:2480	However, the PEG and PEG-MC containing colloids proved to be relatively non-toxic, even at the highest concentration, but with a slowly decreasing tendency of the cell metabolic activity.					
32600693	4	55	dep	polysaccharides	426:440	arg1	MC					479:480	MC	479:480	MC	479:480	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	4	55	dep	polysaccharides	426:440	arg1	Ch					454:455	Ch	454:455	Ch	454:455	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	4	55	dep	polysaccharides	426:440	arg1	Methylcellulose					462:476	Methylcellulose	462:476	Methylcellulose (MC)	462:481	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	4	55	dep	polysaccharides	426:440	arg1	Chitosan					444:451	Chitosan	444:451	Chitosan (Ch)	444:456	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	4	55	dep	polysaccharides	426:440	arg1	polysaccharides					426:440	two naturally occurring polysaccharides	402:440	two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) -	402:483	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	12	56	dep	In	1870:1871	arg1	vitro					1873:1877	vitro	1873:1877	vitro	1873:1877	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	1	57	from	utilization	207:217	arg1	biomedicine					222:232	biomedicine	222:232	biomedicine	222:232	We have developed a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine.					
32600693	5	58	theme	sterilization	795:807	arg1	process					809:815	the sterilization process	791:815	the sterilization process	791:815	The colloids were sterilized by steam, and their stability was measured before and after the sterilization process by dynamic light scattering and UV-Vis spectrophotometry.					
32600693	4	59	theme	sputtering	601:610	arg1	parameters					612:621	the sputtering parameters	597:621	the sputtering parameters	597:621	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	4	60	dep	base	522:525	arg1	improve					639:645	improve	639:645	to further improve the stability and the biocompatibility of the colloid	628:699	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	4	60	dep	base	522:525	arg1	enhance					544:550	enhance	544:550	to enhance the yield of the sputtering without changing the sputtering parameters	541:621	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	12	61	from	tests	1879:1883	arg1	cells					1899:1903	eukaryotic cells	1888:1903	eukaryotic cells	1888:1903	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	4	62	theme	sputtering	569:578	arg1	yield					556:560	the yield	552:560	the yield of the sputtering	552:578	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	12	63	theme	In	1870:1871	arg1	tests					1879:1883	In vitro tests	1870:1883	In vitro tests	1870:1883	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	1	64	theme	simple	129:134	arg1	method					136:141	a novel simple method	121:141	a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine	121:232	We have developed a novel simple method for effective preparing gold nanoparticles (AuNPs) intended for utilization in biomedicine.					
32600693	6	65	theme	sputtering	906:915	arg1	yield					917:921	a higher sputtering yield	897:921	a higher sputtering yield in the colloids containing the polysaccharides	897:968	The results indicated a higher sputtering yield in the colloids containing the polysaccharides.					
32600693	6	66	from	yield	917:921	arg1	colloids					930:937	the colloids	926:937	the colloids containing the polysaccharides	926:968	The results indicated a higher sputtering yield in the colloids containing the polysaccharides.					
32600693	14	67	theme	colloids	2211:2218	arg1	Toxicity					2195:2202	Toxicity	2195:2202	Toxicity of the colloids	2195:2218	Toxicity of the colloids started to reappear at a concentration of 4.5 μg/ml, especially with chitosan in the colloid, where the colloid with a concentration of 6 μg/ml proved to be the most toxic, especially towards the SAOS-2 cell line.					
32600693	7	68	theme	electron	1120:1127	arg1	TEM					1141:1143	TEM	1141:1143	TEM	1141:1143	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	7	68	theme	electron	1120:1127	arg1	microscopy					1129:1138	transmission electron microscopy	1107:1138	transmission electron microscopy (TEM)	1107:1144	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	6	69	contain	containing	939:948	arg2	polysaccharides					954:968	the polysaccharides	950:968	the polysaccharides	950:968	The results indicated a higher sputtering yield in the colloids containing the polysaccharides.					
32600693	6	69	contain	containing	939:948	arg1	colloids					930:937	the colloids	926:937	the colloids containing the polysaccharides	926:968	The results indicated a higher sputtering yield in the colloids containing the polysaccharides.					
32600693	4	70	theme	occurring	416:424	arg1	Methylcellulose					462:476	Methylcellulose	462:476	Methylcellulose (MC)	462:481	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	4	70	theme	occurring	416:424	arg1	Chitosan					444:451	Chitosan	444:451	Chitosan (Ch)	444:456	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	4	70	theme	occurring	416:424	arg1	polysaccharides					426:440	two naturally occurring polysaccharides	402:440	two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) -	402:483	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	7	71	dep	reveal	1051:1056	arg1	evaluate					1184:1191	evaluate	1184:1191	to evaluate their size and clustering	1181:1217	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	7	71	dep	reveal	1051:1056	arg1	visualize					1149:1157	visualize	1149:1157	to visualize the nanoparticles	1146:1175	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	7	71	dep	reveal	1051:1056	arg1	estimate					1240:1247	estimate	1240:1247	to estimate the viscosity of the colloids	1237:1277	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	3	72	theme	AuNP	375:378	arg1	colloid					380:386	the AuNP colloid	371:386	the AuNP colloid	371:386	The PEG was used as a basic biocompatible stabilizer of the AuNP colloid.					
32600693	0	73	theme	new	2:4	arg1	way					6:8	A new way	0:8	A new way	0:8	A new way to prepare gold nanoparticles by sputtering - Sterilization, stability and other properties.					
32600693	9	74	theme	activity	1469:1476	arg1	tests					1446:1450	in vitro tests	1437:1450	in vitro tests of antimicrobial activity and cytotoxicity	1437:1493	In addition, in vitro tests of antimicrobial activity and cytotoxicity were carried out to estimate the biological activity and the biocompatibility of the colloids.					
32600693	9	75	dep	in	1437:1438	arg1	vitro					1440:1444	vitro	1440:1444	vitro	1440:1444	In addition, in vitro tests of antimicrobial activity and cytotoxicity were carried out to estimate the biological activity and the biocompatibility of the colloids.					
32600693	15	76	theme	cell	2597:2600	arg1	activity					2612:2619	the cell metabolic activity	2593:2619	the cell metabolic activity	2593:2619	However, the PEG and PEG-MC containing colloids proved to be relatively non-toxic, even at the highest concentration, but with a slowly decreasing tendency of the cell metabolic activity.					
32600693	11	77	with	AuNP	1744:1747	arg1	chitosan					1754:1761	chitosan	1754:1761	chitosan	1754:1761	AuNP with chitosan proved to possess the highest antibacterial activity, especially towards the Gram-positive S. epidermidis.					
32600693	9	78	theme	cytotoxicity	1482:1493	arg1	tests					1446:1450	in vitro tests	1437:1450	in vitro tests of antimicrobial activity and cytotoxicity	1437:1493	In addition, in vitro tests of antimicrobial activity and cytotoxicity were carried out to estimate the biological activity and the biocompatibility of the colloids.					
32600693	10	79	theme	Antimicrobial	1590:1602	arg1	tests					1604:1608	Antimicrobial tests	1590:1608	Antimicrobial tests	1590:1608	Antimicrobial tests were performed by a drip test on two bacterial strains - Gram-positive Staphylococcus epidermidis and Gram-negative Escherichia coli.					
32600693	0	80	theme	gold	21:24	arg1	nanoparticles					26:38	gold nanoparticles	21:38	gold nanoparticles	21:38	A new way to prepare gold nanoparticles by sputtering - Sterilization, stability and other properties.					
32600693	3	81	used	used	327:330	arg2	stabilizer					357:366	a basic biocompatible stabilizer	335:366	a basic biocompatible stabilizer of the AuNP colloid	335:386	The PEG was used as a basic biocompatible stabilizer of the AuNP colloid.					
32600693	3	81	used	used	327:330	arg2	PEG					319:321	The PEG	315:321	The PEG	315:321	The PEG was used as a basic biocompatible stabilizer of the AuNP colloid.					
32600693	15	82	theme	PEG-MC	2455:2460	arg1	colloids					2473:2480	the PEG and PEG-MC containing colloids	2443:2480	colloids	2473:2480	However, the PEG and PEG-MC containing colloids proved to be relatively non-toxic, even at the highest concentration, but with a slowly decreasing tendency of the cell metabolic activity.					
32600693	3	83	theme	biocompatible	343:355	arg1	stabilizer					357:366	a basic biocompatible stabilizer	335:366	a basic biocompatible stabilizer of the AuNP colloid	335:386	The PEG was used as a basic biocompatible stabilizer of the AuNP colloid.					
32600693	3	83	theme	biocompatible	343:355	arg1	PEG					319:321	The PEG	315:321	The PEG	315:321	The PEG was used as a basic biocompatible stabilizer of the AuNP colloid.					
32600693	12	84	theme	colloids	2090:2097	arg1	effect					2057:2062	the effect	2053:2062	the effect	2053:2062	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	84	theme	colloids	2090:2097	arg1	dose					2078:2081	the toxic dose	2068:2081	the toxic dose of the colloids on human cells	2068:2112	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	7	85	theme	transmission	1107:1118	arg1	TEM					1141:1143	TEM	1141:1143	TEM	1141:1143	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	7	85	theme	transmission	1107:1118	arg1	microscopy					1129:1138	transmission electron microscopy	1107:1138	transmission electron microscopy (TEM)	1107:1144	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
32600693	15	86	theme	decreasing	2570:2579	arg1	tendency					2581:2588	a slowly decreasing tendency	2561:2588	a slowly decreasing tendency of the cell metabolic activity	2561:2619	However, the PEG and PEG-MC containing colloids proved to be relatively non-toxic, even at the highest concentration, but with a slowly decreasing tendency of the cell metabolic activity.					
32600693	14	87	theme	SAOS-2	2416:2421	arg1	line					2428:2431	the SAOS-2 cell line	2412:2431	the SAOS-2 cell line	2412:2431	Toxicity of the colloids started to reappear at a concentration of 4.5 μg/ml, especially with chitosan in the colloid, where the colloid with a concentration of 6 μg/ml proved to be the most toxic, especially towards the SAOS-2 cell line.					
32600693	12	88	theme	human	2102:2106	arg1	cells					2108:2112	human cells	2102:2112	human cells	2102:2112	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	11	89	theme	antibacterial	1793:1805	arg1	activity					1807:1814	the highest antibacterial activity	1781:1814	the highest antibacterial activity	1781:1814	AuNP with chitosan proved to possess the highest antibacterial activity, especially towards the Gram-positive S. epidermidis.					
32600693	12	90	from	cells	2108:2112	arg1	effect					2057:2062	the effect	2053:2062	the effect	2053:2062	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	90	from	cells	2108:2112	arg1	dose					2078:2081	the toxic dose	2068:2081	the toxic dose of the colloids on human cells	2068:2112	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	13	91	theme	colloid	2127:2133	arg1	concentrations					2135:2148	The studied colloid concentrations	2115:2148	The studied colloid concentrations	2115:2148	The studied colloid concentrations were in the range from 0.6 μg/ml to 6 μg/ml.					
32600693	4	92	theme	PEG	518:520	arg1	base					522:525	the PEG base	514:525	the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid	514:699	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	12	93	theme	toxic	2072:2076	arg1	dose					2078:2081	the toxic dose	2068:2081	the toxic dose of the colloids on human cells	2068:2112	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	94	theme	human	1966:1970	arg1	NHDF					1992:1995	NHDF	1992:1995	NHDF	1992:1995	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	12	94	theme	human	1966:1970	arg1	fibroblasts					1979:1989	primary normal human dermal fibroblasts	1951:1989	primary normal human dermal fibroblasts (NHDF)	1951:1996	In vitro tests on eukaryotic cells, i.e. human osteoblastic cell line SAOS-2 and primary normal human dermal fibroblasts (NHDF), were performed after a 7-day cultivation to determine the effect and the toxic dose of the colloids on human cells.					
32600693	4	95	theme	colloid	693:699	arg1	stability					651:659	the stability	647:659	the stability	647:659	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	4	95	theme	colloid	693:699	arg1	biocompatibility					669:684	the biocompatibility	665:684	the biocompatibility of the colloid	665:699	In addition, two naturally occurring polysaccharides - Chitosan (Ch) and Methylcellulose (MC) - were separately diluted into the PEG base with the aims to enhance the yield of the sputtering without changing the sputtering parameters, and to further improve the stability and the biocompatibility of the colloid.					
32600693	7	96	theme	prepared	1081:1088	arg1	nanoparticles					1090:1102	the prepared nanoparticles	1077:1102	the prepared nanoparticles	1077:1102	The colloids were also characterized by atomic absorption spectroscopy (AAS) to reveal the composition of the prepared nanoparticles by transmission electron microscopy (TEM) to visualize the nanoparticles and to evaluate their size and clustering, and by rheometry to estimate the viscosity of the colloids.					
34420759	7	0	theme	1,4-β-mannose	994:1006	arg1	and1					1008:1011	1,4-β-mannose and1	994:1011	1,4-β-mannose and1	994:1011	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	0	1	from	studies	11:17	arg1	Orchidaceae					66:76	Orchidaceae	66:76	Orchidaceae	66:76	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.					
34420759	0	1	from	studies	11:17	arg1	appendiculata					51:63	appendiculata	51:63	appendiculata	51:63	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.					
34420759	7	2	theme	unit	977:980	arg1	substitution					926:937	a 0.208 acetylation substitution	906:937	a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure	906:1045	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	6	3	theme	linear	856:861	arg1	linkage					863:869	a 1,4-β-mannose and 1,4-β-glucose linear linkage	822:869	a 1,4-β-mannose and 1,4-β-glucose linear linkage	822:869	The methylation results indicated that CAP was a 1,4-β-mannose and 1,4-β-glucose linear linkage.					
34420759	6	3	theme	linear	856:861	arg1	CAP					814:816	CAP	814:816	CAP	814:816	The methylation results indicated that CAP was a 1,4-β-mannose and 1,4-β-glucose linear linkage.					
34420759	7	4	theme	further	876:882	arg1	studies					888:894	The further NMR studies	872:894	The further NMR studies	872:894	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	4	5	theme	narrow	566:571	arg1	peak					587:590	a narrow and symmetric peak	564:590	a narrow and symmetric peak presented in the HPGPC	564:613	After isolation by DE-52 and Superdex 200 gel chromatography, the purified polysaccharide (named as CAP) with Mw 557.5 kDa was obtained with a narrow and symmetric peak presented in the HPGPC.					
34420759	8	6	theme	chemical	1052:1059	arg1	structure					1061:1069	The chemical structure	1048:1069	The chemical structure of CAP	1048:1076	The chemical structure of CAP was confirmed further by the specific glucanase and mannanase hydrolysis results.					
34420759	7	7	theme	NMR	884:886	arg1	studies					888:894	The further NMR studies	872:894	The further NMR studies	872:894	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	3	8	theme	fine	354:357	arg1	structure					359:367	fine structure	354:367	fine structure	354:367	Therefore, we focused on the isolation and fine structure characterization of C. appendiculata polysaccharides.					
34420759	0	9	from	appendiculata	51:63	arg1	studies					11:17	Structural studies	0:17	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.	0:112	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.					
34420759	0	9	from	appendiculata	51:63	arg1	mannoglucan					24:34	a mannoglucan	22:34	a mannoglucan from Cremastra appendiculata (Orchidaceae)	22:77	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.					
34420759	7	10	theme	hexasaccharide	952:965	arg1	unit					977:980	a hexasaccharide repeating unit	950:980	a hexasaccharide repeating unit	950:980	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	4	11	theme	symmetric	577:585	arg1	peak					587:590	a narrow and symmetric peak	564:590	a narrow and symmetric peak presented in the HPGPC	564:613	After isolation by DE-52 and Superdex 200 gel chromatography, the purified polysaccharide (named as CAP) with Mw 557.5 kDa was obtained with a narrow and symmetric peak presented in the HPGPC.					
34420759	7	12	theme	repeating	967:975	arg1	unit					977:980	a hexasaccharide repeating unit	950:980	a hexasaccharide repeating unit	950:980	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	2	13	theme	medicinal	272:280	arg1	plant					282:286	this medicinal plant	267:286	this medicinal plant	267:286	The polysaccharides from this medicinal plant are poorly understood.					
34420759	0	14	theme	enzymatic	95:103	arg1	methods					105:111	chemical and enzymatic methods	82:111	chemical and enzymatic methods	82:111	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.					
34420759	0	15	theme	Structural	0:9	arg1	studies					11:17	Structural studies	0:17	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.	0:112	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.					
34420759	4	16	theme	purified	489:496	arg1	polysaccharide					498:511	the purified polysaccharide	485:511	the purified polysaccharide (named as CAP) with Mw 557.5 kDa	485:544	After isolation by DE-52 and Superdex 200 gel chromatography, the purified polysaccharide (named as CAP) with Mw 557.5 kDa was obtained with a narrow and symmetric peak presented in the HPGPC.					
34420759	5	17	theme	monosaccharide	620:633	arg1	composition					635:645	The monosaccharide composition results	616:653	The monosaccharide composition results	616:653	The monosaccharide composition results showed in HPAEC that CAP was a heteropolysaccharide composed of glucose and mannose at a molar ratio roughly 0.34:0.66.					
34420759	6	18	theme	methylation	779:789	arg1	results					791:797	The methylation results	775:797	The methylation results	775:797	The methylation results indicated that CAP was a 1,4-β-mannose and 1,4-β-glucose linear linkage.					
34420759	8	19	theme	specific	1107:1114	arg1	hydrolysis					1140:1149	the specific glucanase and mannanase hydrolysis results	1103:1157	hydrolysis	1140:1149	The chemical structure of CAP was confirmed further by the specific glucanase and mannanase hydrolysis results.					
34420759	3	20	theme	C.	389:390	arg1	polysaccharides					406:420	C. appendiculata polysaccharides	389:420	C. appendiculata polysaccharides	389:420	Therefore, we focused on the isolation and fine structure characterization of C. appendiculata polysaccharides.					
34420759	4	21	dep	polysaccharide	498:511	arg1	named					514:518	named	514:518	named as CAP	514:525	After isolation by DE-52 and Superdex 200 gel chromatography, the purified polysaccharide (named as CAP) with Mw 557.5 kDa was obtained with a narrow and symmetric peak presented in the HPGPC.					
34420759	8	22	theme	CAP	1074:1076	arg1	structure					1061:1069	The chemical structure	1048:1069	The chemical structure of CAP	1048:1076	The chemical structure of CAP was confirmed further by the specific glucanase and mannanase hydrolysis results.					
34420759	1	23	theme	used	185:188	arg1	Pseudobulb					114:123	Pseudobulb	114:123	Pseudobulb of Cremastra appendiculata (Orchidaceae)	114:164	Pseudobulb of Cremastra appendiculata (Orchidaceae) is a traditionally used medicine in China for treatment of certain cancers.					
34420759	1	23	theme	used	185:188	arg1	medicine					190:197	a traditionally used medicine	169:197	a traditionally used medicine in China for treatment of certain cancers	169:239	Pseudobulb of Cremastra appendiculata (Orchidaceae) is a traditionally used medicine in China for treatment of certain cancers.					
34420759	7	24	theme	CAP	942:944	arg1	substitution					926:937	a 0.208 acetylation substitution	906:937	a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure	906:1045	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	7	25	from	4-β-glucose	1014:1024	arg1	structure					1037:1045	the CAP structure	1029:1045	the CAP structure	1029:1045	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	7	26	theme	4-β-glucose	1014:1024	arg1	and1					1008:1011	1,4-β-mannose and1	994:1011	1,4-β-mannose and1	994:1011	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	4	27	with	polysaccharide	498:511	arg1	557.5 kDa					536:544	Mw 557.5 kDa	533:544	Mw 557.5 kDa	533:544	After isolation by DE-52 and Superdex 200 gel chromatography, the purified polysaccharide (named as CAP) with Mw 557.5 kDa was obtained with a narrow and symmetric peak presented in the HPGPC.					
34420759	4	28	theme	Superdex	452:459	arg1	chromatography					469:482	Superdex 200 gel chromatography	452:482	Superdex 200 gel chromatography	452:482	After isolation by DE-52 and Superdex 200 gel chromatography, the purified polysaccharide (named as CAP) with Mw 557.5 kDa was obtained with a narrow and symmetric peak presented in the HPGPC.					
34420759	0	29	theme	mannoglucan	24:34	arg1	studies					11:17	Structural studies	0:17	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.	0:112	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.					
34420759	5	30	dep	composition	635:645	arg1	results					647:653	results	647:653	The monosaccharide composition results	616:653	The monosaccharide composition results showed in HPAEC that CAP was a heteropolysaccharide composed of glucose and mannose at a molar ratio roughly 0.34:0.66.					
34420759	3	31	theme	appendiculata	392:404	arg1	polysaccharides					406:420	C. appendiculata polysaccharides	389:420	C. appendiculata polysaccharides	389:420	Therefore, we focused on the isolation and fine structure characterization of C. appendiculata polysaccharides.					
34420759	8	32	theme	glucanase	1116:1124	arg1	hydrolysis					1140:1149	the specific glucanase and mannanase hydrolysis results	1103:1157	hydrolysis	1140:1149	The chemical structure of CAP was confirmed further by the specific glucanase and mannanase hydrolysis results.					
34420759	4	33	theme	gel	465:467	arg1	chromatography					469:482	Superdex 200 gel chromatography	452:482	Superdex 200 gel chromatography	452:482	After isolation by DE-52 and Superdex 200 gel chromatography, the purified polysaccharide (named as CAP) with Mw 557.5 kDa was obtained with a narrow and symmetric peak presented in the HPGPC.					
34420759	7	34	theme	CAP	1033:1035	arg1	structure					1037:1045	the CAP structure	1029:1045	the CAP structure	1029:1045	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	3	35	theme	polysaccharides	406:420	arg1	isolation					340:348	isolation	340:348	isolation	340:348	Therefore, we focused on the isolation and fine structure characterization of C. appendiculata polysaccharides.					
34420759	3	35	theme	polysaccharides	406:420	arg1	structure					359:367	fine structure	354:367	fine structure	354:367	Therefore, we focused on the isolation and fine structure characterization of C. appendiculata polysaccharides.					
34420759	3	36	dep	isolation	340:348	arg1	characterization					369:384	characterization	369:384	characterization	369:384	Therefore, we focused on the isolation and fine structure characterization of C. appendiculata polysaccharides.					
34420759	3	36	dep	isolation	340:348	arg1	the					336:338	the	336:338	the	336:338	Therefore, we focused on the isolation and fine structure characterization of C. appendiculata polysaccharides.					
34420759	6	37	theme	1,4-β-glucose	842:854	arg1	linkage					863:869	a 1,4-β-mannose and 1,4-β-glucose linear linkage	822:869	a 1,4-β-mannose and 1,4-β-glucose linear linkage	822:869	The methylation results indicated that CAP was a 1,4-β-mannose and 1,4-β-glucose linear linkage.					
34420759	6	37	theme	1,4-β-glucose	842:854	arg1	CAP					814:816	CAP	814:816	CAP	814:816	The methylation results indicated that CAP was a 1,4-β-mannose and 1,4-β-glucose linear linkage.					
34420759	1	38	from	medicine	190:197	arg1	China					202:206	China	202:206	China	202:206	Pseudobulb of Cremastra appendiculata (Orchidaceae) is a traditionally used medicine in China for treatment of certain cancers.					
34420759	7	39	theme	acetylation	914:924	arg1	substitution					926:937	a 0.208 acetylation substitution	906:937	a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure	906:1045	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	4	40	theme	Mw	533:534	arg1	557.5 kDa					536:544	Mw 557.5 kDa	533:544	Mw 557.5 kDa	533:544	After isolation by DE-52 and Superdex 200 gel chromatography, the purified polysaccharide (named as CAP) with Mw 557.5 kDa was obtained with a narrow and symmetric peak presented in the HPGPC.					
34420759	8	41	theme	mannanase	1130:1138	arg1	hydrolysis					1140:1149	the specific glucanase and mannanase hydrolysis results	1103:1157	hydrolysis	1140:1149	The chemical structure of CAP was confirmed further by the specific glucanase and mannanase hydrolysis results.					
34420759	7	42	from	structure	1037:1045	arg1	4-β-glucose					1014:1024	4-β-glucose	1014:1024	4-β-glucose	1014:1024	The further NMR studies suggested a 0.208 acetylation substitution of CAP and a hexasaccharide repeating unit composed of 1,4-β-mannose and1, 4-β-glucose in the CAP structure.					
34420759	6	43	theme	1,4-β-mannose	824:836	arg1	linkage					863:869	a 1,4-β-mannose and 1,4-β-glucose linear linkage	822:869	a 1,4-β-mannose and 1,4-β-glucose linear linkage	822:869	The methylation results indicated that CAP was a 1,4-β-mannose and 1,4-β-glucose linear linkage.					
34420759	6	43	theme	1,4-β-mannose	824:836	arg1	CAP					814:816	CAP	814:816	CAP	814:816	The methylation results indicated that CAP was a 1,4-β-mannose and 1,4-β-glucose linear linkage.					
34420759	8	44	dep	hydrolysis	1140:1149	arg1	results					1151:1157	results	1151:1157	results	1151:1157	The chemical structure of CAP was confirmed further by the specific glucanase and mannanase hydrolysis results.					
34420759	5	45	theme	molar	744:748	arg1	ratio					750:754	a molar ratio	742:754	a molar ratio roughly 0.34:0.66	742:772	The monosaccharide composition results showed in HPAEC that CAP was a heteropolysaccharide composed of glucose and mannose at a molar ratio roughly 0.34:0.66.					
34420759	5	46	from	ratio	750:754	arg1	CAP					676:678	CAP	676:678	CAP	676:678	The monosaccharide composition results showed in HPAEC that CAP was a heteropolysaccharide composed of glucose and mannose at a molar ratio roughly 0.34:0.66.					
34420759	5	46	from	ratio	750:754	arg1	heteropolysaccharide					686:705	a heteropolysaccharide	684:705	a heteropolysaccharide composed of glucose and mannose	684:737	The monosaccharide composition results showed in HPAEC that CAP was a heteropolysaccharide composed of glucose and mannose at a molar ratio roughly 0.34:0.66.					
34420759	1	47	theme	appendiculata	138:150	arg1	Pseudobulb					114:123	Pseudobulb	114:123	Pseudobulb of Cremastra appendiculata (Orchidaceae)	114:164	Pseudobulb of Cremastra appendiculata (Orchidaceae) is a traditionally used medicine in China for treatment of certain cancers.					
34420759	1	47	theme	appendiculata	138:150	arg1	medicine					190:197	a traditionally used medicine	169:197	a traditionally used medicine in China for treatment of certain cancers	169:239	Pseudobulb of Cremastra appendiculata (Orchidaceae) is a traditionally used medicine in China for treatment of certain cancers.					
34420759	2	48	from	plant	282:286	arg1	polysaccharides					246:260	The polysaccharides	242:260	The polysaccharides from this medicinal plant	242:286	The polysaccharides from this medicinal plant are poorly understood.					
34420759	1	49	theme	certain	225:231	arg1	cancers					233:239	certain cancers	225:239	certain cancers	225:239	Pseudobulb of Cremastra appendiculata (Orchidaceae) is a traditionally used medicine in China for treatment of certain cancers.					
34420759	1	50	theme	cancers	233:239	arg1	treatment					212:220	treatment	212:220	treatment of certain cancers	212:239	Pseudobulb of Cremastra appendiculata (Orchidaceae) is a traditionally used medicine in China for treatment of certain cancers.					
34420759	0	51	theme	chemical	82:89	arg1	methods					105:111	chemical and enzymatic methods	82:111	chemical and enzymatic methods	82:111	Structural studies of a mannoglucan from Cremastra appendiculata (Orchidaceae) by chemical and enzymatic methods.					
32691027	5	0	from	value	798:802	arg1	culture					817:823	the fecal culture	807:823	the fecal culture	807:823	The pH value in the fecal culture significantly decreased to 6.68, suggesting that CCPs could be broken down and utilized by gut microbiota.					
32691027	4	1	theme	mg	710:711	arg1	sugar					698:702	reducing sugar	689:702	reducing sugar (1.11 mg mL-1)	689:717	The content of reducing sugar (1.11 mg mL-1) was significantly increased by fermentation in vitro of gut microbiota.					
32691027	4	1	theme	mg	710:711	arg1	mL-1					713:716	1.11 mg mL-1	705:716	1.11 mg mL-1	705:716	The content of reducing sugar (1.11 mg mL-1) was significantly increased by fermentation in vitro of gut microbiota.					
32691027	4	2	theme	gut	775:777	arg1	microbiota					779:788	gut microbiota	775:788	gut microbiota	775:788	The content of reducing sugar (1.11 mg mL-1) was significantly increased by fermentation in vitro of gut microbiota.					
32691027	3	3	theme	antioxidant	509:519	arg1	activity					521:528	the antioxidant activity	505:528	the antioxidant activity throughout this digestion period	505:561	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	6	4	theme	acetic	985:990	arg1	acids					978:982	total short-chain fatty acids	954:982	total short-chain fatty acids	954:982	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	6	4	theme	acetic	985:990	arg1	acids					1017:1021	acetic, propionic and n-butyric acids	985:1021	acetic, propionic and n-butyric acids	985:1021	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	0	5	from	Effects	0:6	arg1	stability					60:68	stability	60:68	stability	60:68	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	0	5	from	Effects	0:6	arg1	behavior					84:91	metabolic behavior	74:91	metabolic behavior	74:91	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	1	6	theme	Craterellus	265:275	arg1	cornucopioides					277:290	Craterellus cornucopioides	265:290	Craterellus cornucopioides	265:290	The present study aimed to investigate the digestion and fermentation behavior of a novel polysaccharide named CCP from Craterellus cornucopioides.					
32691027	0	7	theme	metabolic	74:82	arg1	behavior					84:91	metabolic behavior	74:91	metabolic behavior	74:91	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	3	8	theme	noticeable	484:493	arg1	change					495:500	no noticeable change	481:500	no noticeable change	481:500	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	0	9	theme	polysaccharides	96:110	arg1	stability					60:68	stability	60:68	stability	60:68	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	0	9	theme	polysaccharides	96:110	arg1	behavior					84:91	metabolic behavior	74:91	metabolic behavior	74:91	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	4	10	theme	microbiota	779:788	arg1	fermentation					750:761	fermentation	750:761	fermentation in vitro of gut microbiota	750:788	The content of reducing sugar (1.11 mg mL-1) was significantly increased by fermentation in vitro of gut microbiota.					
32691027	4	11	theme	reducing	689:696	arg1	sugar					698:702	reducing sugar	689:702	reducing sugar (1.11 mg mL-1)	689:717	The content of reducing sugar (1.11 mg mL-1) was significantly increased by fermentation in vitro of gut microbiota.					
32691027	4	11	theme	reducing	689:696	arg1	mL-1					713:716	1.11 mg mL-1	705:716	1.11 mg mL-1	705:716	The content of reducing sugar (1.11 mg mL-1) was significantly increased by fermentation in vitro of gut microbiota.					
32691027	6	12	theme	acids	978:982	arg1	concentrations					936:949	The concentrations	932:949	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids	932:1021	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	0	13	from	cornucopioides	129:142	arg1	stability					60:68	stability	60:68	stability	60:68	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	0	13	from	cornucopioides	129:142	arg1	behavior					84:91	metabolic behavior	74:91	metabolic behavior	74:91	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	0	13	from	cornucopioides	129:142	arg1	polysaccharides					96:110	polysaccharides	96:110	polysaccharides from Craterellus cornucopioides	96:142	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	3	14	theme	glucose	430:436	arg1	monosaccharide					438:451	free glucose monosaccharide	425:451	free glucose monosaccharide	425:451	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	6	15	theme	n-butyric	1007:1015	arg1	acids					978:982	total short-chain fatty acids	954:982	total short-chain fatty acids	954:982	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	6	15	theme	n-butyric	1007:1015	arg1	acids					1017:1021	acetic, propionic and n-butyric acids	985:1021	acetic, propionic and n-butyric acids	985:1021	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	6	16	theme	fatty	972:976	arg1	acids					978:982	total short-chain fatty acids	954:982	total short-chain fatty acids	954:982	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	6	16	theme	fatty	972:976	arg1	acids					1017:1021	acetic, propionic and n-butyric acids	985:1021	acetic, propionic and n-butyric acids	985:1021	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	0	17	dep	stability	60:68	arg1	the					56:58	the	56:58	the	56:58	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	3	18	theme	monosaccharide	438:451	arg1	amount					415:420	only a small amount	402:420	only a small amount of free glucose monosaccharide	402:451	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	3	18	theme	monosaccharide	438:451	arg1	monosaccharide					438:451	free glucose monosaccharide	425:451	free glucose monosaccharide	425:451	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	5	19	theme	fecal	811:815	arg1	culture					817:823	the fecal culture	807:823	the fecal culture	807:823	The pH value in the fecal culture significantly decreased to 6.68, suggesting that CCPs could be broken down and utilized by gut microbiota.					
32691027	6	20	theme	short-chain	960:970	arg1	acids					978:982	total short-chain fatty acids	954:982	total short-chain fatty acids	954:982	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	6	20	theme	short-chain	960:970	arg1	acids					1017:1021	acetic, propionic and n-butyric acids	985:1021	acetic, propionic and n-butyric acids	985:1021	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	0	21	theme	Craterellus	117:127	arg1	cornucopioides					129:142	Craterellus cornucopioides	117:142	Craterellus cornucopioides	117:142	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	5	22	theme	gut	916:918	arg1	microbiota					920:929	gut microbiota	916:929	gut microbiota	916:929	The pH value in the fecal culture significantly decreased to 6.68, suggesting that CCPs could be broken down and utilized by gut microbiota.					
32691027	5	23	theme	pH	795:796	arg1	value					798:802	The pH value	791:802	The pH value in the fecal culture	791:823	The pH value in the fecal culture significantly decreased to 6.68, suggesting that CCPs could be broken down and utilized by gut microbiota.					
32691027	0	24	theme	in	11:12	arg1	digestion					20:28	in vitro digestion	11:28	in vitro digestion	11:28	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	6	25	theme	total	954:958	arg1	acids					978:982	total short-chain fatty acids	954:982	total short-chain fatty acids	954:982	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	6	25	theme	total	954:958	arg1	acids					1017:1021	acetic, propionic and n-butyric acids	985:1021	acetic, propionic and n-butyric acids	985:1021	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	2	26	theme	in	315:316	arg1	digestion					324:332	in vitro digestion	315:332	in vitro digestion	315:332	Results revealed that in vitro digestion could significantly decrease the molecular weight of CCPs.					
32691027	7	27	theme	microbiota	1161:1170	arg1	abundance					1137:1145	abundance	1137:1145	abundance	1137:1145	Simultaneously, CCPs could remarkably modulate the composition and abundance of beneficial microbiota, especially Bacteroidetes.					
32691027	7	27	theme	microbiota	1161:1170	arg1	composition					1121:1131	composition	1121:1131	composition	1121:1131	Simultaneously, CCPs could remarkably modulate the composition and abundance of beneficial microbiota, especially Bacteroidetes.					
32691027	1	28	theme	fermentation	202:213	arg1	behavior					215:222	fermentation behavior	202:222	fermentation behavior	202:222	The present study aimed to investigate the digestion and fermentation behavior of a novel polysaccharide named CCP from Craterellus cornucopioides.					
32691027	7	29	theme	beneficial	1150:1159	arg1	microbiota					1161:1170	beneficial microbiota	1150:1170	beneficial microbiota	1150:1170	Simultaneously, CCPs could remarkably modulate the composition and abundance of beneficial microbiota, especially Bacteroidetes.					
32691027	0	30	from	stability	60:68	arg1	cornucopioides					129:142	Craterellus cornucopioides	117:142	Craterellus cornucopioides	117:142	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	3	31	theme	CCPs	605:608	arg1	stable					625:630	stable	625:630	stable	625:630	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	3	31	theme	CCPs	605:608	arg1	structure					592:600	the main structure	583:600	the main structure of CCPs	583:608	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	3	32	theme	main	587:590	arg1	stable					625:630	stable	625:630	stable	625:630	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	3	32	theme	main	587:590	arg1	structure					592:600	the main structure	583:600	the main structure of CCPs	583:608	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	0	33	theme	digestion	20:28	arg1	Effects					0:6	Effects	0:6	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.	0:143	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	3	34	theme	free	425:428	arg1	monosaccharide					438:451	free glucose monosaccharide	425:451	free glucose monosaccharide	425:451	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	3	35	theme	simulated	642:650	arg1	conditions					662:671	the simulated digestive conditions	638:671	the simulated digestive conditions	638:671	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	1	36	dep	digestion	188:196	arg1	the					184:186	the	184:186	the	184:186	The present study aimed to investigate the digestion and fermentation behavior of a novel polysaccharide named CCP from Craterellus cornucopioides.					
32691027	7	37	dep	composition	1121:1131	arg1	the					1117:1119	the	1117:1119	the	1117:1119	Simultaneously, CCPs could remarkably modulate the composition and abundance of beneficial microbiota, especially Bacteroidetes.					
32691027	0	38	theme	fermentation	40:51	arg1	Effects					0:6	Effects	0:6	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.	0:143	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	3	39	theme	digestive	652:660	arg1	conditions					662:671	the simulated digestive conditions	638:671	the simulated digestive conditions	638:671	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	2	40	theme	molecular	367:375	arg1	weight					377:382	the molecular weight	363:382	the molecular weight of CCPs	363:390	Results revealed that in vitro digestion could significantly decrease the molecular weight of CCPs.					
32691027	0	41	theme	fecal	34:38	arg1	fermentation					40:51	fecal fermentation	34:51	fecal fermentation	34:51	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	2	42	dep	in	315:316	arg1	vitro					318:322	vitro	318:322	vitro	318:322	Results revealed that in vitro digestion could significantly decrease the molecular weight of CCPs.					
32691027	4	43	theme	sugar	698:702	arg1	content					678:684	The content	674:684	The content of reducing sugar (1.11 mg mL-1)	674:717	The content of reducing sugar (1.11 mg mL-1) was significantly increased by fermentation in vitro of gut microbiota.					
32691027	4	44	theme	1.11	705:708	arg1	mg					710:711	mg	710:711	mg	710:711	The content of reducing sugar (1.11 mg mL-1) was significantly increased by fermentation in vitro of gut microbiota.					
32691027	3	45	theme	digestion	546:554	arg1	period					556:561	this digestion period	541:561	this digestion period	541:561	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	0	46	from	behavior	84:91	arg1	cornucopioides					129:142	Craterellus cornucopioides	117:142	Craterellus cornucopioides	117:142	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	0	47	dep	in	11:12	arg1	vitro					14:18	vitro	14:18	vitro	14:18	Effects of in vitro digestion and fecal fermentation on the stability and metabolic behavior of polysaccharides from Craterellus cornucopioides.					
32691027	1	48	theme	present	149:155	arg1	study					157:161	The present study	145:161	The present study	145:161	The present study aimed to investigate the digestion and fermentation behavior of a novel polysaccharide named CCP from Craterellus cornucopioides.					
32691027	8	49	theme	functional	1238:1247	arg1	CCPs					1210:1213	CCPs	1210:1213	CCPs	1210:1213	Therefore, CCPs could potentially be a functional food to prevent disease by promoting gut health.					
32691027	8	49	theme	functional	1238:1247	arg1	food					1249:1252	a functional food	1236:1252	a functional food to prevent disease by promoting gut health	1236:1295	Therefore, CCPs could potentially be a functional food to prevent disease by promoting gut health.					
32691027	1	50	theme	novel	229:233	arg1	polysaccharide					235:248	a novel polysaccharide	227:248	a novel polysaccharide named CCP from Craterellus cornucopioides	227:290	The present study aimed to investigate the digestion and fermentation behavior of a novel polysaccharide named CCP from Craterellus cornucopioides.					
32691027	8	51	theme	gut	1286:1288	arg1	health					1290:1295	gut health	1286:1295	gut health	1286:1295	Therefore, CCPs could potentially be a functional food to prevent disease by promoting gut health.					
32691027	5	52	used	utilized	904:911	arg2	CCPs					874:877	CCPs	874:877	CCPs	874:877	The pH value in the fecal culture significantly decreased to 6.68, suggesting that CCPs could be broken down and utilized by gut microbiota.					
32691027	1	53	theme	polysaccharide	235:248	arg1	digestion					188:196	digestion	188:196	digestion	188:196	The present study aimed to investigate the digestion and fermentation behavior of a novel polysaccharide named CCP from Craterellus cornucopioides.					
32691027	1	53	theme	polysaccharide	235:248	arg1	behavior					215:222	fermentation behavior	202:222	fermentation behavior	202:222	The present study aimed to investigate the digestion and fermentation behavior of a novel polysaccharide named CCP from Craterellus cornucopioides.					
32691027	3	54	theme	small	409:413	arg1	amount					415:420	only a small amount	402:420	only a small amount of free glucose monosaccharide	402:451	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	3	54	theme	small	409:413	arg1	monosaccharide					438:451	free glucose monosaccharide	425:451	free glucose monosaccharide	425:451	However, only a small amount of free glucose monosaccharide was released, and there was no noticeable change in the antioxidant activity throughout this digestion period, demonstrating that the main structure of CCPs was relatively stable under the simulated digestive conditions.					
32691027	2	55	theme	CCPs	387:390	arg1	weight					377:382	the molecular weight	363:382	the molecular weight of CCPs	363:390	Results revealed that in vitro digestion could significantly decrease the molecular weight of CCPs.					
32691027	6	56	theme	propionic	993:1001	arg1	acids					978:982	total short-chain fatty acids	954:982	total short-chain fatty acids	954:982	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
32691027	6	56	theme	propionic	993:1001	arg1	acids					1017:1021	acetic, propionic and n-butyric acids	985:1021	acetic, propionic and n-butyric acids	985:1021	The concentrations of total short-chain fatty acids, acetic, propionic and n-butyric acids significantly increased compared to the blank.					
33140034	6	0	theme	conformational	1028:1041	arg1	dynamics					1043:1050	the conformational dynamics	1024:1050	the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	1024:1138	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	6	1	from	N234	1005:1008	arg1	N-glycans					977:985	N-glycans	977:985	N-glycans at sites N165 and N234	977:1008	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	6	1	from	N234	1005:1008	arg1	role					969:972	an essential structural role	945:972	an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	945:1138	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	2	2	theme	spike	350:354	arg1	protein					360:366	the extensively glycosylated SARS-CoV-2 spike (S) protein	310:366	the extensively glycosylated SARS-CoV-2 spike (S) protein	310:366	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
33140034	7	3	theme	shift	1362:1366	arg1	result					1329:1334	a result	1327:1334	a result of the RBD conformational shift toward the "down" state	1327:1390	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift toward the "down" state.					
33140034	7	3	theme	shift	1362:1366	arg1	binding					1308:1314	binding	1308:1314	binding to ACE2	1308:1322	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift toward the "down" state.					
33140034	3	4	theme	SARS-CoV-2	507:516	arg1	spike					518:522	the SARS-CoV-2 spike	503:522	the SARS-CoV-2 spike	503:522	Similar to many other viral fusion proteins, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
33140034	7	5	theme	"	1384:1384	arg1	state					1386:1390	the "down" state	1375:1390	the "down" state	1375:1390	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift toward the "down" state.					
33140034	7	6	theme	RBD	1343:1345	arg1	shift					1362:1366	the RBD conformational shift	1339:1366	the RBD conformational shift toward the "down" state	1339:1390	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift toward the "down" state.					
33140034	4	7	theme	full-length	602:612	arg1	model					614:618	a full-length model	600:618	a full-length model of the glycosylated SARS-CoV-2 S protein	600:659	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	5	8	used	used	827:830	arg2	simulations					810:820	Multiple microsecond-long, all-atom molecular dynamics simulations	755:820	Multiple microsecond-long, all-atom molecular dynamics simulations	755:820	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	6	9	theme	domain	1084:1089	arg1	dynamics					1043:1050	the conformational dynamics	1024:1050	the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	1024:1138	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	3	10	theme	other	474:478	arg1	proteins					493:500	many other viral fusion proteins	469:500	many other viral fusion proteins	469:500	Similar to many other viral fusion proteins, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
33140034	5	11	theme	dynamics	801:808	arg1	simulations					810:820	Multiple microsecond-long, all-atom molecular dynamics simulations	755:820	Multiple microsecond-long, all-atom molecular dynamics simulations	755:820	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	4	12	gly	glycosylated	627:638	arg1	protein					653:659	the glycosylated SARS-CoV-2 S protein	623:659	the glycosylated SARS-CoV-2 S protein	623:659	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	1	13	theme	severe	112:117	arg1	SARS-CoV-2					161:170	SARS-CoV-2	161:170	SARS-CoV-2	161:170	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	1	13	theme	severe	112:117	arg1	coronavirus					146:156	severe acute respiratory syndrome coronavirus 2	112:158	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	112:171	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	9	14	theme	functional	1670:1679	arg1	insights					1696:1703	hitherto unseen functional and structural insights	1654:1703	hitherto unseen functional and structural insights into the SARS-CoV-2 S protein	1654:1733	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
33140034	4	15	theme	glycosylated	627:638	arg1	protein					653:659	the glycosylated SARS-CoV-2 S protein	623:659	the glycosylated SARS-CoV-2 S protein	623:659	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	3	16	theme	immune	568:573	arg1	response					575:582	the host immune response	559:582	the host immune response	559:582	Similar to many other viral fusion proteins, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
33140034	1	17	theme	respiratory	125:135	arg1	SARS-CoV-2					161:170	SARS-CoV-2	161:170	SARS-CoV-2	161:170	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	1	17	theme	respiratory	125:135	arg1	coronavirus					146:156	severe acute respiratory syndrome coronavirus 2	112:158	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	112:171	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	6	18	theme	essential	948:956	arg1	role					969:972	an essential structural role	945:972	an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	945:1138	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	9	19	theme	structural	1685:1694	arg1	insights					1696:1703	hitherto unseen functional and structural insights	1654:1703	hitherto unseen functional and structural insights into the SARS-CoV-2 S protein	1654:1733	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
33140034	8	20	theme	end-to-end	1407:1416	arg1	analyses					1432:1439	end-to-end accessibility analyses	1407:1439	end-to-end accessibility analyses	1407:1439	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine.					
33140034	4	21	theme	S	651:651	arg1	protein					653:659	the glycosylated SARS-CoV-2 S protein	623:659	the glycosylated SARS-CoV-2 S protein	623:659	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	0	22	theme	Spike	57:61	arg1	Protein					63:69	the SARS-CoV-2 Spike Protein	42:69	the SARS-CoV-2 Spike Protein	42:69	Beyond Shielding: The Roles of Glycans in the SARS-CoV-2 Spike Protein.					
33140034	3	23	dep	glycan	535:540	arg1	shield					542:547	shield	542:547	shield	542:547	Similar to many other viral fusion proteins, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
33140034	2	24	theme	Antibody	259:266	arg1	development					268:278	Antibody development	259:278	Antibody development	259:278	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
33140034	9	25	theme	SARS-CoV-2	1714:1723	arg1	protein					1727:1733	the SARS-CoV-2 S protein	1710:1733	the SARS-CoV-2 S protein	1710:1733	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
33140034	2	26	theme	angiotensin-converting	418:439	arg1	enzyme					441:446	angiotensin-converting enzyme 2	418:448	the angiotensin-converting enzyme 2 (ACE2)	414:455	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
33140034	2	26	theme	angiotensin-converting	418:439	arg1	ACE2					451:454	ACE2	451:454	ACE2	451:454	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
33140034	0	27	from	Roles	22:26	arg1	Protein					63:69	the SARS-CoV-2 Spike Protein	42:69	the SARS-CoV-2 Spike Protein	42:69	Beyond Shielding: The Roles of Glycans in the SARS-CoV-2 Spike Protein.					
33140034	4	28	theme	open	674:677	arg1	states					690:695	the open and closed states	670:695	the open and closed states	670:695	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	4	29	theme	closed	683:688	arg1	states					690:695	the open and closed states	670:695	the open and closed states	670:695	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	5	30	theme	microsecond-long	764:779	arg1	simulations					810:820	Multiple microsecond-long, all-atom molecular dynamics simulations	755:820	Multiple microsecond-long, all-atom molecular dynamics simulations	755:820	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	1	31	theme	COVID-19	84:91	arg1	pandemic					93:100	The ongoing COVID-19 pandemic	72:100	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	72:171	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	2	32	theme	cell	389:392	arg1	entry					394:398	host cell entry	384:398	host cell entry	384:398	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
33140034	6	33	theme	N-glycans	977:985	arg1	role					969:972	an essential structural role	945:972	an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	945:1138	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	2	34	gly	glycosylated	326:337	arg1	protein					360:366	the extensively glycosylated SARS-CoV-2 spike (S) protein	310:366	the extensively glycosylated SARS-CoV-2 spike (S) protein	310:366	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
33140034	0	35	theme	Glycans	31:37	arg1	Roles					22:26	The Roles	18:26	The Roles of Glycans in the SARS-CoV-2 Spike Protein	18:69	Beyond Shielding: The Roles of Glycans in the SARS-CoV-2 Spike Protein.					
33140034	0	36	dep	Roles	22:26	arg1	Shielding					7:15	Shielding	7:15	Shielding	7:15	Beyond Shielding: The Roles of Glycans in the SARS-CoV-2 Spike Protein.					
33140034	4	37	theme	structural	723:732	arg1	data					749:752	the available structural and biological data	709:752	the available structural and biological data	709:752	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	8	38	theme	SARS-CoV-2	1520:1529	arg1	protein					1533:1539	the SARS-CoV-2 S protein	1516:1539	the SARS-CoV-2 S protein	1516:1539	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine.					
33140034	4	39	theme	biological	738:747	arg1	data					749:752	the available structural and biological data	709:752	the available structural and biological data	709:752	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	3	40	theme	viral	480:484	arg1	proteins					493:500	many other viral fusion proteins	469:500	many other viral fusion proteins	469:500	Similar to many other viral fusion proteins, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
33140034	8	41	theme	therapeutic	1572:1582	arg1	efforts					1584:1590	the therapeutic efforts	1568:1590	the therapeutic efforts targeting this molecular machine	1568:1623	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine.					
33140034	7	42	theme	interferometry	1182:1195	arg1	experiments					1197:1207	biolayer interferometry experiments	1173:1207	biolayer interferometry experiments	1173:1207	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift toward the "down" state.					
33140034	9	43	theme	conformational	1792:1805	arg1	plasticity					1807:1816	the conformational plasticity	1788:1816	the conformational plasticity of the RBD that could be harnessed for vaccine development	1788:1875	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
33140034	8	44	theme	protein	1533:1539	arg1	shield					1506:1511	shield	1506:1511	shield	1506:1511	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine.					
33140034	3	45	theme	many	469:472	arg1	proteins					493:500	many other viral fusion proteins	469:500	many other viral fusion proteins	469:500	Similar to many other viral fusion proteins, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
33140034	5	46	dep	microsecond-long	764:779	arg1	all-atom					782:789	all-atom	782:789	all-atom	782:789	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	8	47	theme	molecular	1607:1615	arg1	machine					1617:1623	this molecular machine	1602:1623	this molecular machine	1602:1623	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine.					
33140034	1	48	theme	worldwide	240:248	arg1	infections					210:219	more than 28,000,000 infections	189:219	more than 28,000,000 infections	189:219	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	5	49	theme	atomistic	846:854	arg1	perspective					856:866	an atomistic perspective	843:866	an atomistic perspective on the roles of glycans and on the protein structure and dynamics	843:932	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	9	50	theme	RBD	1825:1827	arg1	plasticity					1807:1816	the conformational plasticity	1788:1816	the conformational plasticity of the RBD that could be harnessed for vaccine development	1788:1875	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
33140034	7	51	theme	conformational	1347:1360	arg1	shift					1362:1366	the RBD conformational shift	1339:1366	the RBD conformational shift toward the "down" state	1339:1390	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift toward the "down" state.					
33140034	6	52	from	sites	990:994	arg1	N-glycans					977:985	N-glycans	977:985	N-glycans at sites N165 and N234	977:1008	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	6	52	from	sites	990:994	arg1	role					969:972	an essential structural role	945:972	an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	945:1138	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	6	53	theme	binding	1076:1082	arg1	RBD					1092:1094	RBD	1092:1094	RBD	1092:1094	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	6	53	theme	binding	1076:1082	arg1	domain					1084:1089	the spike's receptor binding domain	1055:1089	the spike's receptor binding domain (RBD)	1055:1095	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	9	54	theme	vaccine	1857:1863	arg1	development					1865:1875	vaccine development	1857:1875	vaccine development	1857:1875	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
33140034	5	55	theme	molecular	791:799	arg1	simulations					810:820	Multiple microsecond-long, all-atom molecular dynamics simulations	755:820	Multiple microsecond-long, all-atom molecular dynamics simulations	755:820	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	4	56	theme	protein	653:659	arg1	model					614:618	a full-length model	600:618	a full-length model of the glycosylated SARS-CoV-2 S protein	600:659	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	9	57	theme	unseen	1663:1668	arg1	insights					1696:1703	hitherto unseen functional and structural insights	1654:1703	hitherto unseen functional and structural insights into the SARS-CoV-2 S protein	1654:1733	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
33140034	6	58	theme	ACE2	1123:1126	arg1	recognition					1128:1138	ACE2 recognition	1123:1138	ACE2 recognition	1123:1138	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	0	59	theme	SARS-CoV-2	46:55	arg1	Protein					63:69	the SARS-CoV-2 Spike Protein	42:69	the SARS-CoV-2 Spike Protein	42:69	Beyond Shielding: The Roles of Glycans in the SARS-CoV-2 Spike Protein.					
33140034	3	60	theme	host	563:566	arg1	response					575:582	the host immune response	559:582	the host immune response	559:582	Similar to many other viral fusion proteins, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
33140034	1	61	theme	acute	119:123	arg1	SARS-CoV-2					161:170	SARS-CoV-2	161:170	SARS-CoV-2	161:170	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	1	61	theme	acute	119:123	arg1	coronavirus					146:156	severe acute respiratory syndrome coronavirus 2	112:158	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	112:171	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	6	62	theme	structural	958:967	arg1	role					969:972	an essential structural role	945:972	an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	945:1138	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	8	63	theme	accessibility	1418:1430	arg1	analyses					1432:1439	end-to-end accessibility analyses	1407:1439	end-to-end accessibility analyses	1407:1439	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine.					
33140034	1	64	theme	syndrome	137:144	arg1	SARS-CoV-2					161:170	SARS-CoV-2	161:170	SARS-CoV-2	161:170	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	1	64	theme	syndrome	137:144	arg1	coronavirus					146:156	severe acute respiratory syndrome coronavirus 2	112:158	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	112:171	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	4	65	theme	SARS-CoV-2	640:649	arg1	protein					653:659	the glycosylated SARS-CoV-2 S protein	623:659	the glycosylated SARS-CoV-2 S protein	623:659	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	5	66	from	perspective	856:866	arg1	structure					911:919	the protein structure	899:919	the protein structure	899:919	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	5	66	from	perspective	856:866	arg1	roles					875:879	the roles	871:879	the roles of glycans	871:890	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	5	66	from	perspective	856:866	arg1	dynamics					925:932	dynamics	925:932	dynamics	925:932	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	5	67	theme	protein	903:909	arg1	structure					911:919	the protein structure	899:919	the protein structure	899:919	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	8	68	theme	complete	1451:1458	arg1	overview					1460:1467	a complete overview	1449:1467	a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine	1449:1623	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine.					
33140034	6	69	from	role	969:972	arg1	sites					990:994	sites N165 and N234	990:1008	sites	990:994	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	6	69	from	role	969:972	arg1	N234					1005:1008	N234	1005:1008	N234	1005:1008	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	2	70	dep	spike	350:354	arg1	S					357:357	S	357:357	S	357:357	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
33140034	9	71	theme	S	1725:1725	arg1	protein					1727:1733	the SARS-CoV-2 S protein	1710:1733	the SARS-CoV-2 S protein	1710:1733	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
33140034	5	72	theme	glycans	884:890	arg1	roles					875:879	the roles	871:879	the roles of glycans	871:890	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	7	73	theme	glycans	1244:1250	arg1	deletion					1226:1233	deletion	1226:1233	deletion of these glycans through N165A and N234A mutations	1226:1284	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift toward the "down" state.					
33140034	8	74	theme	vulnerabilities	1476:1490	arg1	overview					1460:1467	a complete overview	1449:1467	a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine	1449:1623	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine.					
33140034	5	75	theme	Multiple	755:762	arg1	simulations					810:820	Multiple microsecond-long, all-atom molecular dynamics simulations	755:820	Multiple microsecond-long, all-atom molecular dynamics simulations	755:820	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans and on the protein structure and dynamics.					
33140034	1	76	theme	ongoing	76:82	arg1	pandemic					93:100	The ongoing COVID-19 pandemic	72:100	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	72:171	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 28,000,000 infections and 900,000 deaths worldwide to date.					
33140034	9	77	theme	glycan	1743:1748	arg1	coat					1750:1753	its glycan coat	1739:1753	its glycan coat	1739:1753	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
33140034	4	78	dep	built	594:598	arg1	both					662:665	both	662:665	both	662:665	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	8	79	theme	shield	1506:1511	arg1	vulnerabilities					1476:1490	the vulnerabilities	1472:1490	the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine	1472:1623	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine.					
33140034	2	80	theme	host	384:387	arg1	entry					394:398	host cell entry	384:398	host cell entry	384:398	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
33140034	7	81	dep	N165A	1260:1264	arg1	mutations					1276:1284	mutations	1276:1284	mutations	1276:1284	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift toward the "down" state.					
33140034	6	82	theme	receptor	1067:1074	arg1	RBD					1092:1094	RBD	1092:1094	RBD	1092:1094	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	6	82	theme	receptor	1067:1074	arg1	domain					1084:1089	the spike's receptor binding domain	1055:1089	the spike's receptor binding domain (RBD)	1055:1095	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
33140034	2	83	theme	glycosylated	326:337	arg1	protein					360:366	the extensively glycosylated SARS-CoV-2 spike (S) protein	310:366	the extensively glycosylated SARS-CoV-2 spike (S) protein	310:366	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
33140034	8	84	theme	S	1531:1531	arg1	protein					1533:1539	the SARS-CoV-2 S protein	1516:1539	the SARS-CoV-2 S protein	1516:1539	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of the SARS-CoV-2 S protein, which may be exploited in the therapeutic efforts targeting this molecular machine.					
33140034	4	85	theme	available	713:721	arg1	data					749:752	the available structural and biological data	709:752	the available structural and biological data	709:752	Here, we built a full-length model of the glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
33140034	7	86	theme	biolayer	1173:1180	arg1	experiments					1197:1207	biolayer interferometry experiments	1173:1207	biolayer interferometry experiments	1173:1207	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift toward the "down" state.					
33140034	3	87	theme	fusion	486:491	arg1	proteins					493:500	many other viral fusion proteins	469:500	many other viral fusion proteins	469:500	Similar to many other viral fusion proteins, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
34354526	0	0	theme	Mucin-degrading	68:82	arg1	Microbes					88:95	Mucin-degrading Gut Microbes	68:95	Mucin-degrading Gut Microbes	68:95	Sialylated O -Glycans from Hen Egg White Ovomucin are Decomposed by Mucin-degrading Gut Microbes.					
34354526	3	1	theme	heavy	458:462	arg1	modification					464:475	heavy modification	458:475	heavy modification by N -acetylneuraminic acid	458:503	O- Glycan analysis revealed that ovomucin contained core-1 and 2 structures with heavy modification by N -acetylneuraminic acid and/or sulfate groups.					
34354526	1	2	theme	O	223:223	arg1	-glycosylation					225:238	extensive O -glycosylation	213:238	extensive O -glycosylation with a high degree of sialylation	213:272	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	6	3	theme	gut	1088:1090	arg1	microbes					1092:1099	gut microbes	1088:1099	gut microbes	1088:1099	Our data suggest that ovomucin potentially affects the gut microbiota through O -glycan decomposition by gut microbes and degradant sugar sharing within the community.					
34354526	5	4	theme	species-dependent	854:870	arg1	manner					872:877	a species-dependent manner	852:877	a species-dependent manner	852:877	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	4	5	dep	microbes	559:566	arg1	we					568:569	we	568:569	we	568:569	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	4	6	theme	sole	643:646	arg1	source					655:660	a sole carbon source	641:660	a sole carbon source	641:660	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	0	7	theme	Gut	84:86	arg1	Microbes					88:95	Mucin-degrading Gut Microbes	68:95	Mucin-degrading Gut Microbes	68:95	Sialylated O -Glycans from Hen Egg White Ovomucin are Decomposed by Mucin-degrading Gut Microbes.					
34354526	3	8	theme	sulfate	512:518	arg1	groups					520:525	sulfate groups	512:525	sulfate groups	512:525	O- Glycan analysis revealed that ovomucin contained core-1 and 2 structures with heavy modification by N -acetylneuraminic acid and/or sulfate groups.					
34354526	2	9	theme	used	289:292	arg1	ingredient					299:308	a commonly used food ingredient	278:308	a commonly used food ingredient	278:308	As a commonly used food ingredient, we explored whether ovomucin has an effect on the gut microbiota.					
34354526	5	10	dep	microbes	742:749	arg1	Both					733:736	Both	733:736	Both	733:736	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	3	11	theme	-acetylneuraminic	482:498	arg1	acid					500:503	N -acetylneuraminic acid	480:503	N -acetylneuraminic acid	480:503	O- Glycan analysis revealed that ovomucin contained core-1 and 2 structures with heavy modification by N -acetylneuraminic acid and/or sulfate groups.					
34354526	6	12	theme	-glycan	1063:1069	arg1	decomposition					1071:1083	O -glycan decomposition	1061:1083	O -glycan decomposition by gut microbes and degradant sugar sharing within the community	1061:1148	Our data suggest that ovomucin potentially affects the gut microbiota through O -glycan decomposition by gut microbes and degradant sugar sharing within the community.					
34354526	4	13	theme	gut	555:557	arg1	microbes					559:566	the two mucin-degrading gut microbes	531:566	the two mucin-degrading gut microbes we tested	531:576	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	0	14	from	Egg	31:33	arg1	-Glycans					13:20	Sialylated O -Glycans	0:20	Sialylated O -Glycans from Hen Egg White Ovomucin	0:48	Sialylated O -Glycans from Hen Egg White Ovomucin are Decomposed by Mucin-degrading Gut Microbes.					
34354526	4	15	theme	mucin-degrading	539:553	arg1	microbes					559:566	the two mucin-degrading gut microbes	531:566	the two mucin-degrading gut microbes we tested	531:576	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	5	16	theme	exchange	949:956	arg1	chromatography					958:971	anion exchange chromatography	943:971	anion exchange chromatography analysis	943:980	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	6	17	theme	O	1061:1061	arg1	decomposition					1071:1083	O -glycan decomposition	1061:1083	O -glycan decomposition by gut microbes and degradant sugar sharing within the community	1061:1148	Our data suggest that ovomucin potentially affects the gut microbiota through O -glycan decomposition by gut microbes and degradant sugar sharing within the community.					
34354526	0	18	theme	O	11:11	arg1	-Glycans					13:20	Sialylated O -Glycans	0:20	Sialylated O -Glycans from Hen Egg White Ovomucin	0:48	Sialylated O -Glycans from Hen Egg White Ovomucin are Decomposed by Mucin-degrading Gut Microbes.					
34354526	3	19	theme	O-	377:378	arg1	analysis					387:394	O- Glycan analysis	377:394	O- Glycan analysis	377:394	O- Glycan analysis revealed that ovomucin contained core-1 and 2 structures with heavy modification by N -acetylneuraminic acid and/or sulfate groups.					
34354526	0	20	theme	Sialylated	0:9	arg1	-Glycans					13:20	Sialylated O -Glycans	0:20	Sialylated O -Glycans from Hen Egg White Ovomucin	0:48	Sialylated O -Glycans from Hen Egg White Ovomucin are Decomposed by Mucin-degrading Gut Microbes.					
34354526	1	21	theme	high	247:250	arg1	degree					252:257	a high degree	245:257	a high degree of sialylation	245:272	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	4	22	theme	h	674:674	arg1	period					684:689	a 24 h culture period	669:689	a 24 h culture period	669:689	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	5	23	theme	semi-quantified	895:909	arg1	analysis					930:937	semi-quantified mass spectrometric analysis	895:937	semi-quantified mass spectrometric analysis	895:937	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	1	24	theme	hydrogel-forming	157:172	arg1	properties					174:183	its hydrogel-forming properties	153:183	its hydrogel-forming properties	153:183	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	4	25	theme	Bifidobacterium	700:714	arg1	bifidum					716:722	Bifidobacterium bifidum	700:722	Bifidobacterium bifidum	700:722	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	5	26	theme	O	779:779	arg1	-glycans					781:788	ovomucin O -glycans	770:788	ovomucin O -glycans	770:788	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	5	27	theme	ovomucin	770:777	arg1	-glycans					781:788	ovomucin O -glycans	770:788	ovomucin O -glycans	770:788	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	5	28	theme	anion	943:947	arg1	chromatography					958:971	anion exchange chromatography	943:971	anion exchange chromatography analysis	943:980	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	2	29	contain	has	340:342	arg2	effect					347:352	an effect	344:352	an effect on the gut microbiota	344:374	As a commonly used food ingredient, we explored whether ovomucin has an effect on the gut microbiota.					
34354526	2	29	contain	has	340:342	arg1	ovomucin					331:338	ovomucin	331:338	ovomucin	331:338	As a commonly used food ingredient, we explored whether ovomucin has an effect on the gut microbiota.					
34354526	5	30	theme	spectrometric	916:928	arg1	analysis					930:937	semi-quantified mass spectrometric analysis	895:937	semi-quantified mass spectrometric analysis	895:937	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	4	31	theme	carbon	648:653	arg1	source					655:660	a sole carbon source	641:660	a sole carbon source	641:660	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	1	32	theme	sialylation	262:272	arg1	degree					252:257	a high degree	245:257	a high degree of sialylation	245:272	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	1	33	with	properties	174:183	arg1	degree					252:257	a high degree	245:257	a high degree of sialylation	245:272	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	1	34	theme	high	186:189	arg1	weight					201:206	high molecular weight	186:206	high molecular weight	186:206	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	3	35	theme	Glycan	380:385	arg1	analysis					387:394	O- Glycan analysis	377:394	O- Glycan analysis	377:394	O- Glycan analysis revealed that ovomucin contained core-1 and 2 structures with heavy modification by N -acetylneuraminic acid and/or sulfate groups.					
34354526	2	36	theme	gut	361:363	arg1	microbiota					365:374	the gut microbiota	357:374	the gut microbiota	357:374	As a commonly used food ingredient, we explored whether ovomucin has an effect on the gut microbiota.					
34354526	5	37	theme	gut	738:740	arg1	microbes					742:749	Both gut microbes	733:749	microbes	742:749	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	1	38	theme	molecular	191:199	arg1	weight					201:206	high molecular weight	186:206	high molecular weight	186:206	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	3	39	contain	contained	419:427	arg1	ovomucin					410:417	ovomucin	410:417	ovomucin	410:417	O- Glycan analysis revealed that ovomucin contained core-1 and 2 structures with heavy modification by N -acetylneuraminic acid and/or sulfate groups.					
34354526	3	39	contain	contained	419:427	arg2	structures					442:451	core-1 and 2 structures	429:451	core-1 and 2 structures	429:451	O- Glycan analysis revealed that ovomucin contained core-1 and 2 structures with heavy modification by N -acetylneuraminic acid and/or sulfate groups.					
34354526	0	40	dep	Egg	31:33	arg1	Ovomucin					41:48	Ovomucin	41:48	Ovomucin	41:48	Sialylated O -Glycans from Hen Egg White Ovomucin are Decomposed by Mucin-degrading Gut Microbes.					
34354526	6	41	theme	degradant	1105:1113	arg1	sugar					1115:1119	degradant sugar	1105:1119	degradant sugar	1105:1119	Our data suggest that ovomucin potentially affects the gut microbiota through O -glycan decomposition by gut microbes and degradant sugar sharing within the community.					
34354526	4	42	theme	24	671:672	arg1	h					674:674	h	674:674	h	674:674	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	0	43	theme	White	35:39	arg1	Egg					31:33	Hen Egg White Ovomucin	27:48	Hen Egg White Ovomucin	27:48	Sialylated O -Glycans from Hen Egg White Ovomucin are Decomposed by Mucin-degrading Gut Microbes.					
34354526	4	44	theme	culture	676:682	arg1	period					684:689	a 24 h culture period	669:689	a 24 h culture period	669:689	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	5	45	theme	culture	828:834	arg1	supernatants					836:847	the culture supernatants	824:847	the culture supernatants	824:847	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	5	46	theme	chromatography	958:971	arg1	analysis					973:980	anion exchange chromatography analysis	943:980	anion exchange chromatography analysis	943:980	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	1	47	with	-glycosylation	225:238	arg1	degree					252:257	a high degree	245:257	a high degree of sialylation	245:272	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	1	48	theme	hen	110:112	arg1	protein					124:130	a hen egg white protein	108:130	a hen egg white protein	108:130	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	1	48	theme	hen	110:112	arg1	Ovomucin					98:105	Ovomucin	98:105	Ovomucin	98:105	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	5	49	theme	mass	911:914	arg1	analysis					930:937	semi-quantified mass spectrometric analysis	895:937	semi-quantified mass spectrometric analysis	895:937	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	2	50	from	effect	347:352	arg1	microbiota					365:374	the gut microbiota	357:374	the gut microbiota	357:374	As a commonly used food ingredient, we explored whether ovomucin has an effect on the gut microbiota.					
34354526	4	51	contain	containing	618:627	arg2	ovomucin					629:636	ovomucin	629:636	ovomucin	629:636	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	4	51	contain	containing	618:627	arg1	medium					611:616	medium	611:616	medium containing ovomucin	611:636	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	1	52	theme	egg	114:116	arg1	protein					124:130	a hen egg white protein	108:130	a hen egg white protein	108:130	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	1	52	theme	egg	114:116	arg1	Ovomucin					98:105	Ovomucin	98:105	Ovomucin	98:105	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	2	53	theme	food	294:297	arg1	ingredient					299:308	a commonly used food ingredient	278:308	a commonly used food ingredient	278:308	As a commonly used food ingredient, we explored whether ovomucin has an effect on the gut microbiota.					
34354526	5	54	theme	released	794:801	arg1	monosaccharides					803:817	released monosaccharides	794:817	released monosaccharides	794:817	Both gut microbes, however, degraded ovomucin O -glycans and released monosaccharides into the culture supernatants in a species-dependent manner, as revealed by semi-quantified mass spectrometric analysis and anion exchange chromatography analysis.					
34354526	6	55	theme	gut	1038:1040	arg1	microbiota					1042:1051	the gut microbiota	1034:1051	the gut microbiota	1034:1051	Our data suggest that ovomucin potentially affects the gut microbiota through O -glycan decomposition by gut microbes and degradant sugar sharing within the community.					
34354526	1	56	theme	white	118:122	arg1	protein					124:130	a hen egg white protein	108:130	a hen egg white protein	108:130	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	1	56	theme	white	118:122	arg1	Ovomucin					98:105	Ovomucin	98:105	Ovomucin	98:105	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	4	57	theme	Akkermansia	579:589	arg1	muciniphila					591:601	Akkermansia muciniphila	579:601	Akkermansia muciniphila	579:601	Of the two mucin-degrading gut microbes we tested, Akkermansia muciniphila grew in medium containing ovomucin as a sole carbon source during a 24 h culture period, whereas Bifidobacterium bifidum did not.					
34354526	1	58	with	weight	201:206	arg1	degree					252:257	a high degree	245:257	a high degree of sialylation	245:272	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
34354526	3	59	theme	N	480:480	arg1	acid					500:503	N -acetylneuraminic acid	480:503	N -acetylneuraminic acid	480:503	O- Glycan analysis revealed that ovomucin contained core-1 and 2 structures with heavy modification by N -acetylneuraminic acid and/or sulfate groups.					
34354526	1	60	theme	extensive	213:221	arg1	-glycosylation					225:238	extensive O -glycosylation	213:238	extensive O -glycosylation with a high degree of sialylation	213:272	Ovomucin, a hen egg white protein, is characterized by its hydrogel-forming properties, high molecular weight, and extensive O -glycosylation with a high degree of sialylation.					
33211056	3	0	theme	IgE	660:662	arg1	levels					664:669	serum OVA-specific IgE levels	641:669	serum OVA-specific IgE levels	641:669	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	3	1	theme	fatty	591:595	arg1	acids					597:601	short-chain fatty acids	579:601	short-chain fatty acids' (SCFAs) metabolism	579:621	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	3	1	theme	fatty	591:595	arg1	SCFAs					605:609	SCFAs	605:609	SCFAs	605:609	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	7	2	theme	intestinal	1301:1310	arg1	microbiota					1312:1321	the intestinal microbiota	1297:1321	the intestinal microbiota	1297:1321	More importantly, this may be closely related to alterations in the intestinal microbiota.					
33211056	6	3	theme	action	1085:1090	arg1	maintaining					1151:1161	maintaining	1151:1161	maintaining immune homeostasis in newborns, which may partly last into adulthood	1151:1230	Therefore, we speculate that the mechanism of action of the indigestible carbohydrate fibers of WGPs is through maintaining immune homeostasis in newborns, which may partly last into adulthood.					
33211056	6	3	theme	action	1085:1090	arg1	mechanism					1072:1080	the mechanism	1068:1080	the mechanism of action of the indigestible carbohydrate fibers of WGPs	1068:1138	Therefore, we speculate that the mechanism of action of the indigestible carbohydrate fibers of WGPs is through maintaining immune homeostasis in newborns, which may partly last into adulthood.					
33211056	0	4	theme	mice	67:70	arg1	response					55:62	the immune response	44:62	the immune response of mice from newborn to adulthood	44:96	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood associated with intestinal microbiota.					
33211056	5	5	from	levels	863:868	arg1	mice					909:912	the WGP-treated mice	893:912	the WGP-treated mice	893:912	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	0	6	from	Regulation	0:9	arg1	response					55:62	the immune response	44:62	the immune response of mice from newborn to adulthood	44:96	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood associated with intestinal microbiota.					
33211056	2	7	dep	microbiota	434:443	arg1	both					458:461	both	458:461	both	458:461	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	4	8	theme	ceftriaxone-treated	716:734	arg1	mice					736:739	ceftriaxone-treated mice	716:739	ceftriaxone-treated mice	716:739	Comparing adulthood mice to ceftriaxone-treated mice, the diversity and composition of intestinal microbiota were significantly improved after WGP treatment.					
33211056	2	9	theme	adulthood	478:486	arg1	mice					488:491	newborn and adulthood mice	466:491	newborn and adulthood mice	466:491	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	3	10	theme	newborn	674:680	arg1	mice					682:685	newborn mice	674:685	newborn mice	674:685	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	1	11	from	mice	273:276	arg1	life					287:290	early life	281:290	early life	281:290	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	6	12	theme	WGPs	1135:1138	arg1	fibers					1125:1130	the indigestible carbohydrate fibers	1095:1130	the indigestible carbohydrate fibers of WGPs	1095:1138	Therefore, we speculate that the mechanism of action of the indigestible carbohydrate fibers of WGPs is through maintaining immune homeostasis in newborns, which may partly last into adulthood.					
33211056	6	13	theme	indigestible	1099:1110	arg1	fibers					1125:1130	the indigestible carbohydrate fibers	1095:1130	the indigestible carbohydrate fibers of WGPs	1095:1138	Therefore, we speculate that the mechanism of action of the indigestible carbohydrate fibers of WGPs is through maintaining immune homeostasis in newborns, which may partly last into adulthood.					
33211056	1	14	theme	study	153:157	arg1	aim					141:143	The aim	137:143	The aim of this study	137:157	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	2	15	theme	newborn	466:472	arg1	mice					488:491	newborn and adulthood mice	466:491	newborn and adulthood mice	466:491	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	2	16	dep	life	385:388	arg1	ceftriaxone					390:400	ceftriaxone	390:400	ceftriaxone	390:400	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	2	16	dep	life	385:388	arg1	treatment					410:418	treatment	410:418	treatment	410:418	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	2	17	theme	WGP	406:408	arg1	influences					365:374	the influences	361:374	the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice	361:491	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	1	18	theme	long-lasting	320:331	arg1	consequences					333:344	long-lasting consequences	320:344	long-lasting consequences	320:344	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	4	19	theme	WGP	831:833	arg1	treatment					835:843	WGP treatment	831:843	WGP treatment	831:843	Comparing adulthood mice to ceftriaxone-treated mice, the diversity and composition of intestinal microbiota were significantly improved after WGP treatment.					
33211056	5	20	theme	cytokines	966:974	arg1	expression					952:961	the expression	948:961	the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α)	948:1011	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	5	21	theme	WGP-treated	897:907	arg1	mice					909:912	the WGP-treated mice	893:912	the WGP-treated mice	893:912	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	7	22	from	alterations	1282:1292	arg1	microbiota					1312:1321	the intestinal microbiota	1297:1321	the intestinal microbiota	1297:1321	More importantly, this may be closely related to alterations in the intestinal microbiota.					
33211056	4	23	dep	diversity	746:754	arg1	the					742:744	the	742:744	the	742:744	Comparing adulthood mice to ceftriaxone-treated mice, the diversity and composition of intestinal microbiota were significantly improved after WGP treatment.					
33211056	1	24	theme	indigestible	236:247	arg1	fibers					262:267	indigestible carbohydrate fibers	236:267	indigestible carbohydrate fibers	236:267	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	1	24	theme	indigestible	236:247	arg1	polysaccharides					202:216	wheat germ polysaccharides	191:216	wheat germ polysaccharides (WGPs)	191:223	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	3	25	theme	organ	624:628	arg1	index					630:634	organ index	624:634	organ index	624:634	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	1	26	from	changes	301:307	arg1	mice					273:276	mice	273:276	mice in early life	273:290	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	1	27	theme	carbohydrate	249:260	arg1	fibers					262:267	indigestible carbohydrate fibers	236:267	indigestible carbohydrate fibers	236:267	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	1	27	theme	carbohydrate	249:260	arg1	polysaccharides					202:216	wheat germ polysaccharides	191:216	wheat germ polysaccharides (WGPs)	191:223	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	0	28	theme	intestinal	114:123	arg1	microbiota					125:134	intestinal microbiota	114:134	intestinal microbiota	114:134	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood associated with intestinal microbiota.					
33211056	4	29	theme	microbiota	786:795	arg1	composition					760:770	composition	760:770	composition	760:770	Comparing adulthood mice to ceftriaxone-treated mice, the diversity and composition of intestinal microbiota were significantly improved after WGP treatment.					
33211056	4	29	theme	microbiota	786:795	arg1	diversity					746:754	diversity	746:754	diversity	746:754	Comparing adulthood mice to ceftriaxone-treated mice, the diversity and composition of intestinal microbiota were significantly improved after WGP treatment.					
33211056	5	30	from	IgE	886:888	arg1	mice					909:912	the WGP-treated mice	893:912	the WGP-treated mice	893:912	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	2	31	theme	early	379:383	arg1	life					385:388	early life ceftriaxone and WGP treatment	379:418	life	385:388	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	2	32	theme	life	385:388	arg1	influences					365:374	the influences	361:374	the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice	361:491	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	4	33	theme	adulthood	698:706	arg1	mice					708:711	adulthood mice	698:711	adulthood mice	698:711	Comparing adulthood mice to ceftriaxone-treated mice, the diversity and composition of intestinal microbiota were significantly improved after WGP treatment.					
33211056	4	34	theme	intestinal	775:784	arg1	microbiota					786:795	intestinal microbiota	775:795	intestinal microbiota	775:795	Comparing adulthood mice to ceftriaxone-treated mice, the diversity and composition of intestinal microbiota were significantly improved after WGP treatment.					
33211056	6	35	theme	fibers	1125:1130	arg1	action					1085:1090	action	1085:1090	action of the indigestible carbohydrate fibers of WGPs	1085:1138	Therefore, we speculate that the mechanism of action of the indigestible carbohydrate fibers of WGPs is through maintaining immune homeostasis in newborns, which may partly last into adulthood.					
33211056	0	36	theme	polysaccharides	25:39	arg1	Regulation					0:9	Regulation	0:9	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood	0:96	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood associated with intestinal microbiota.					
33211056	2	37	theme	intestinal	423:432	arg1	microbiota					434:443	intestinal microbiota	423:443	intestinal microbiota	423:443	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	0	38	theme	germ	20:23	arg1	polysaccharides					25:39	wheat germ polysaccharides	14:39	wheat germ polysaccharides	14:39	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood associated with intestinal microbiota.					
33211056	1	39	theme	wheat	191:195	arg1	germ					197:200	wheat germ	191:200	wheat germ polysaccharides (WGPs)	191:223	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	0	40	theme	immune	48:53	arg1	response					55:62	the immune response	44:62	the immune response of mice from newborn to adulthood	44:96	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood associated with intestinal microbiota.					
33211056	0	41	from	response	55:62	arg1	newborn					77:83	newborn	77:83	newborn	77:83	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood associated with intestinal microbiota.					
33211056	5	42	theme	OVA-specific	873:884	arg1	IgE					886:888	OVA-specific IgE	873:888	OVA-specific IgE in the WGP-treated mice	873:912	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	3	43	theme	serum	641:645	arg1	levels					664:669	serum OVA-specific IgE levels	641:669	serum OVA-specific IgE levels	641:669	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	6	44	theme	immune	1163:1168	arg1	homeostasis					1170:1180	immune homeostasis	1163:1180	immune homeostasis	1163:1180	Therefore, we speculate that the mechanism of action of the indigestible carbohydrate fibers of WGPs is through maintaining immune homeostasis in newborns, which may partly last into adulthood.					
33211056	3	45	theme	intestinal	556:565	arg1	microbiota					567:576	the intestinal microbiota	552:576	the intestinal microbiota	552:576	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	5	46	theme	IgE	886:888	arg1	levels					863:868	the levels	859:868	the levels of OVA-specific IgE in the WGP-treated mice	859:912	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	1	47	theme	early	281:285	arg1	life					287:290	early life	281:290	early life	281:290	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	3	48	theme	acids	597:601	arg1	metabolism					612:621	short-chain fatty acids' (SCFAs) metabolism	579:621	short-chain fatty acids' (SCFAs) metabolism	579:621	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	1	49	from	effects	180:186	arg1	mice					273:276	mice	273:276	mice in early life	273:290	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	1	50	theme	germ	197:200	arg1	fibers					262:267	indigestible carbohydrate fibers	236:267	indigestible carbohydrate fibers	236:267	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	1	50	theme	germ	197:200	arg1	WGPs					219:222	WGPs	219:222	WGPs	219:222	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	1	50	theme	germ	197:200	arg1	polysaccharides					202:216	wheat germ polysaccharides	191:216	wheat germ polysaccharides (WGPs)	191:223	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	6	51	theme	carbohydrate	1112:1123	arg1	fibers					1125:1130	the indigestible carbohydrate fibers	1095:1130	the indigestible carbohydrate fibers of WGPs	1095:1138	Therefore, we speculate that the mechanism of action of the indigestible carbohydrate fibers of WGPs is through maintaining immune homeostasis in newborns, which may partly last into adulthood.					
33211056	2	52	from	influences	365:374	arg1	microbiota					434:443	intestinal microbiota	423:443	intestinal microbiota	423:443	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	2	52	from	influences	365:374	arg1	mice					488:491	newborn and adulthood mice	466:491	newborn and adulthood mice	466:491	We determined the influences of early life ceftriaxone and WGP treatment on intestinal microbiota and immunity both in newborn and adulthood mice.					
33211056	1	53	theme	polysaccharides	202:216	arg1	effects					180:186	the effects	176:186	the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life	176:290	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	1	53	theme	polysaccharides	202:216	arg1	changes					301:307	the changes	297:307	the changes leading to long-lasting consequences	297:344	The aim of this study was to determine the effects of wheat germ polysaccharides (WGPs), which are indigestible carbohydrate fibers, on mice in early life, and the changes leading to long-lasting consequences.					
33211056	0	54	from	newborn	77:83	arg1	mice					67:70	mice	67:70	mice from newborn	67:83	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood associated with intestinal microbiota.					
33211056	0	54	from	newborn	77:83	arg1	response					55:62	the immune response	44:62	the immune response of mice from newborn to adulthood	44:96	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood associated with intestinal microbiota.					
33211056	3	55	theme	short-chain	579:589	arg1	acids					597:601	short-chain fatty acids	579:601	short-chain fatty acids' (SCFAs) metabolism	579:621	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	3	55	theme	short-chain	579:589	arg1	SCFAs					605:609	SCFAs	605:609	SCFAs	605:609	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	5	56	dep	cytokines	966:974	arg1	cytokines					966:974	cytokines	966:974	cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α)	966:1011	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	5	56	dep	cytokines	966:974	arg1	IL-6					989:992	IL-6	989:992	IL-6	989:992	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	5	56	dep	cytokines	966:974	arg1	IFN-γ					995:999	IFN-γ	995:999	IFN-γ	995:999	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	5	56	dep	cytokines	966:974	arg1	IL-2					977:980	IL-2	977:980	IL-2	977:980	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	5	56	dep	cytokines	966:974	arg1	IL-4					983:986	IL-4	983:986	IL-4	983:986	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	5	56	dep	cytokines	966:974	arg1	TNF-α					1006:1010	TNF-α	1006:1010	TNF-α	1006:1010	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	5	57	from	mice	909:912	arg1	levels					863:868	the levels	859:868	the levels of OVA-specific IgE in the WGP-treated mice	859:912	In addition, the levels of OVA-specific IgE in the WGP-treated mice were significantly decreased, and the expression of cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were obviously increased.					
33211056	3	58	theme	OVA-specific	647:658	arg1	levels					664:669	serum OVA-specific IgE levels	641:669	serum OVA-specific IgE levels	641:669	The results showed that ceftriaxone significantly altered the intestinal microbiota, short-chain fatty acids' (SCFAs) metabolism, organ index, and serum OVA-specific IgE levels in newborn mice.					
33211056	0	59	theme	wheat	14:18	arg1	polysaccharides					25:39	wheat germ polysaccharides	14:39	wheat germ polysaccharides	14:39	Regulation of wheat germ polysaccharides in the immune response of mice from newborn to adulthood associated with intestinal microbiota.					
33096174	3	0	located	detected	669:676	arg1	content					685:691	the content	681:691	the content of carbohydrates (80.64 ± 0.98%), protein (1.80 ± 0.28%), fat (0.04 ± 0.005%) and ash (6.32 ± 0.26%)	681:792	Remarkable variability was detected in the content of carbohydrates (80.64 ± 0.98%), protein (1.80 ± 0.28%), fat (0.04 ± 0.005%) and ash (6.32 ± 0.26%).					
33096174	3	0	located	detected	669:676	arg2	variability					653:663	Remarkable variability	642:663	Remarkable variability	642:663	Remarkable variability was detected in the content of carbohydrates (80.64 ± 0.98%), protein (1.80 ± 0.28%), fat (0.04 ± 0.005%) and ash (6.32 ± 0.26%).					
33096174	4	1	theme	similar	875:881	arg1	characteristics					894:908	similar structural characteristics	875:908	similar structural characteristics	875:908	FT-IR assay and 1H and 13C NMR spectroscopy revealed that fruits extract showed similar structural characteristics.					
33096174	1	2	theme	in	538:539	arg1	assays					559:564	in vitro and in vivo assays	538:564	their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays	448:564	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	0	3	dep	in	142:143	arg1	vitro					145:149	vitro	145:149	vitro	145:149	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	2	4	theme	extraction	571:580	arg1	yield					582:586	The extraction yield	567:586	The extraction yield of PSTF (4.26%)	567:602	The extraction yield of PSTF (4.26%) was higher than that of PSMF (3.56%).					
33096174	2	4	theme	extraction	571:580	arg1	higher					608:613	higher	608:613	higher	608:613	The extraction yield of PSTF (4.26%) was higher than that of PSMF (3.56%).					
33096174	6	5	contain	contained	1055:1063	arg2	%					1120:1120	31.21-41.15%	1109:1120	31.21-41.15%	1109:1120	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	6	5	contain	contained	1055:1063	arg2	%					1147:1147	10.90-17.63%	1136:1147	10.90-17.63%	1136:1147	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	6	5	contain	contained	1055:1063	arg2	fucose					1128:1133	fucose	1128:1133	fucose (10.90-17.63%)	1128:1148	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	6	5	contain	contained	1055:1063	arg2	acid					1103:1106	arabinose (40.55-42.03%) galacturonic acid	1065:1106	arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%)	1065:1121	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	6	5	contain	contained	1055:1063	arg1	Both					1050:1053	Both	1050:1053	Both	1050:1053	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	6	6	dep	had	1160:1162	arg1	%					1177:1177	9.13%	1173:1177	9.13%	1173:1177	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	0	7	from	insights	17:24	arg1	polysaccharides					29:43	polysaccharides	29:43	polysaccharides from Schinus terebinthifolius and Schinus molle fruits	29:98	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	2	8	theme	PSTF	591:594	arg1	yield					582:586	The extraction yield	567:586	The extraction yield of PSTF (4.26%)	567:602	The extraction yield of PSTF (4.26%) was higher than that of PSMF (3.56%).					
33096174	2	8	theme	PSTF	591:594	arg1	higher					608:613	higher	608:613	higher	608:613	The extraction yield of PSTF (4.26%) was higher than that of PSMF (3.56%).					
33096174	12	9	theme	in	1703:1704	arg1	capacities					1749:1758	interesting in vivo antinociceptive and anti-inflammatory capacities	1691:1758	interesting in vivo antinociceptive and anti-inflammatory capacities	1691:1758	PSTF and PSMF revealed interesting in vivo antinociceptive and anti-inflammatory capacities.					
33096174	12	10	dep	in	1703:1704	arg1	vivo					1706:1709	vivo	1706:1709	vivo	1706:1709	PSTF and PSMF revealed interesting in vivo antinociceptive and anti-inflammatory capacities.					
33096174	12	10	dep	in	1703:1704	arg1	anti-inflammatory					1731:1747	anti-inflammatory	1731:1747	anti-inflammatory	1731:1747	PSTF and PSMF revealed interesting in vivo antinociceptive and anti-inflammatory capacities.					
33096174	12	10	dep	in	1703:1704	arg1	antinociceptive					1711:1725	antinociceptive	1711:1725	antinociceptive	1711:1725	PSTF and PSMF revealed interesting in vivo antinociceptive and anti-inflammatory capacities.					
33096174	0	11	theme	anti-inflammatory	239:255	arg1	properties					278:287	in vivo anti-inflammatory and anti-nociceptive properties	231:287	in vivo anti-inflammatory and anti-nociceptive properties	231:287	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	7	12	theme	emulsifying	1317:1327	arg1	properties					1329:1338	emulsifying properties	1317:1338	emulsifying properties	1317:1338	Functional analyses demonstrated that samples exhibited favorable water- and oil-retention capacity, emulsifying properties, and foaming qualities.					
33096174	6	13	contain	had	1193:1195	arg1	PSMF					1188:1191	PSMF	1188:1191	PSMF	1188:1191	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	6	13	contain	had	1193:1195	arg2	galactose					1197:1205	galactose	1197:1205	galactose	1197:1205	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	0	14	theme	Physicochemical	101:115	arg1	profiles					132:139	Physicochemical and functional profiles	101:139	Physicochemical and functional profiles	101:139	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	7	15	theme	foaming	1345:1351	arg1	qualities					1353:1361	foaming qualities	1345:1361	foaming qualities	1345:1361	Functional analyses demonstrated that samples exhibited favorable water- and oil-retention capacity, emulsifying properties, and foaming qualities.					
33096174	0	16	theme	antihemolytic	201:213	arg1	capacities					215:224	anti-genotoxicity, antidiabetic, and antihemolytic capacities	164:224	anti-genotoxicity, antidiabetic, and antihemolytic capacities	164:224	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	2	17	dep	higher	608:613	arg1	%					638:638	3.56%	634:638	3.56%	634:638	The extraction yield of PSTF (4.26%) was higher than that of PSMF (3.56%).					
33096174	9	18	theme	remarkable	1434:1443	arg1	effect					1467:1472	a remarkable in vitro antidiabetic effect	1432:1472	a remarkable in vitro antidiabetic effect	1432:1472	Both of them showed a remarkable in vitro antidiabetic effect.					
33096174	4	19	dep	1H	811:812	arg1	spectroscopy					826:837	NMR spectroscopy	822:837	NMR spectroscopy	822:837	FT-IR assay and 1H and 13C NMR spectroscopy revealed that fruits extract showed similar structural characteristics.					
33096174	0	20	from	terebinthifolius	58:73	arg1	polysaccharides					29:43	polysaccharides	29:43	polysaccharides from Schinus terebinthifolius and Schinus molle fruits	29:98	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	0	21	theme	anti-nociceptive	261:276	arg1	properties					278:287	in vivo anti-inflammatory and anti-nociceptive properties	231:287	in vivo anti-inflammatory and anti-nociceptive properties	231:287	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	9	22	theme	in	1445:1446	arg1	effect					1467:1472	a remarkable in vitro antidiabetic effect	1432:1472	a remarkable in vitro antidiabetic effect	1432:1472	Both of them showed a remarkable in vitro antidiabetic effect.					
33096174	0	23	theme	functional	121:130	arg1	profiles					132:139	Physicochemical and functional profiles	101:139	Physicochemical and functional profiles	101:139	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	11	24	theme	extracted	1567:1575	arg1	polysaccharides					1577:1591	The extracted polysaccharides	1563:1591	The extracted polysaccharides	1563:1591	The extracted polysaccharides showed potent inhibitory activity against AAPH-induced plasmid DNA damage.					
33096174	10	25	theme	H2O2-induced	1497:1508	arg1	hemolysis					1510:1518	H2O2-induced hemolysis	1497:1518	H2O2-induced hemolysis	1497:1518	PSMF highly mitigated H2O2-induced hemolysis and exhibited ~80% antihemolytic activity.					
33096174	1	26	theme	in	551:552	arg1	assays					559:564	in vitro and in vivo assays	538:564	their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays	448:564	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	0	27	dep	in	231:232	arg1	vivo					234:237	vivo	234:237	vivo	234:237	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	6	28	theme	galacturonic	1090:1101	arg1	acid					1103:1106	arabinose (40.55-42.03%) galacturonic acid	1065:1106	arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%)	1065:1121	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	6	28	theme	galacturonic	1090:1101	arg1	%					1120:1120	31.21-41.15%	1109:1120	31.21-41.15%	1109:1120	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	11	29	theme	potent	1600:1605	arg1	activity					1618:1625	potent inhibitory activity	1600:1625	potent inhibitory activity against AAPH-induced plasmid DNA damage	1600:1665	The extracted polysaccharides showed potent inhibitory activity against AAPH-induced plasmid DNA damage.					
33096174	6	30	dep	galacturonic	1090:1101	arg1	%					1087:1087	40.55-42.03%	1076:1087	40.55-42.03%	1076:1087	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	0	31	theme	Multidirectional	0:15	arg1	insights					17:24	Multidirectional insights	0:24	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.	0:288	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	3	32	theme	carbohydrates	696:708	arg1	content					685:691	the content	681:691	the content of carbohydrates (80.64 ± 0.98%), protein (1.80 ± 0.28%), fat (0.04 ± 0.005%) and ash (6.32 ± 0.26%)	681:792	Remarkable variability was detected in the content of carbohydrates (80.64 ± 0.98%), protein (1.80 ± 0.28%), fat (0.04 ± 0.005%) and ash (6.32 ± 0.26%).					
33096174	0	33	theme	in	231:232	arg1	properties					278:287	in vivo anti-inflammatory and anti-nociceptive properties	231:287	in vivo anti-inflammatory and anti-nociceptive properties	231:287	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	1	34	theme	physicochemical	466:480	arg1	structures					454:463	their structures	448:463	their structures	448:463	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	1	34	theme	physicochemical	466:480	arg1	characteristics					482:496	physicochemical characteristics	466:496	physicochemical characteristics	466:496	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	7	35	theme	oil-retention	1293:1305	arg1	capacity					1307:1314	favorable water- and oil-retention capacity	1272:1314	favorable water- and oil-retention capacity	1272:1314	Functional analyses demonstrated that samples exhibited favorable water- and oil-retention capacity, emulsifying properties, and foaming qualities.					
33096174	5	36	theme	monosaccharide	985:998	arg1	composition					1000:1010	the monosaccharide composition	981:1010	the monosaccharide composition	981:1010	Thin layer chromatography together with HPLC-RID analysis showed that the monosaccharide composition varied significantly between species.					
33096174	6	37	dep	had	1193:1195	arg1	%					1212:1212	7.40%	1208:1212	7.40%	1208:1212	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	1	38	theme	current	305:311	arg1	study					313:317	the current study	301:317	the current study	301:317	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	0	39	theme	in	142:143	arg1	antioxidant					151:161	in vitro antioxidant	142:161	in vitro antioxidant	142:161	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	7	40	theme	favorable	1272:1280	arg1	capacity					1307:1314	favorable water- and oil-retention capacity	1272:1314	favorable water- and oil-retention capacity	1272:1314	Functional analyses demonstrated that samples exhibited favorable water- and oil-retention capacity, emulsifying properties, and foaming qualities.					
33096174	1	41	dep	in	538:539	arg1	vitro					541:545	vitro	541:545	vitro	541:545	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	1	42	theme	study	313:317	arg1	aim					294:296	The aim	290:296	The aim of the current study	290:317	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	7	43	theme	water-	1282:1287	arg1	capacity					1307:1314	favorable water- and oil-retention capacity	1272:1314	favorable water- and oil-retention capacity	1272:1314	Functional analyses demonstrated that samples exhibited favorable water- and oil-retention capacity, emulsifying properties, and foaming qualities.					
33096174	10	44	theme	%	1537:1537	arg1	activity					1553:1560	~80% antihemolytic activity	1534:1560	~80% antihemolytic activity	1534:1560	PSMF highly mitigated H2O2-induced hemolysis and exhibited ~80% antihemolytic activity.					
33096174	9	45	theme	antidiabetic	1454:1465	arg1	effect					1467:1472	a remarkable in vitro antidiabetic effect	1432:1472	a remarkable in vitro antidiabetic effect	1432:1472	Both of them showed a remarkable in vitro antidiabetic effect.					
33096174	11	46	theme	AAPH-induced	1635:1646	arg1	damage					1660:1665	AAPH-induced plasmid DNA damage	1635:1665	AAPH-induced plasmid DNA damage	1635:1665	The extracted polysaccharides showed potent inhibitory activity against AAPH-induced plasmid DNA damage.					
33096174	0	47	from	fruits	93:98	arg1	polysaccharides					29:43	polysaccharides	29:43	polysaccharides from Schinus terebinthifolius and Schinus molle fruits	29:98	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	5	48	theme	layer	916:920	arg1	chromatography					922:935	Thin layer chromatography	911:935	Thin layer chromatography together with HPLC-RID analysis	911:967	Thin layer chromatography together with HPLC-RID analysis showed that the monosaccharide composition varied significantly between species.					
33096174	6	49	contain	had	1160:1162	arg1	PSTF					1155:1158	PSTF	1155:1158	PSTF	1155:1158	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	6	49	contain	had	1160:1162	arg2	glucose					1164:1170	glucose	1164:1170	glucose	1164:1170	Both contained arabinose (40.55-42.03%) galacturonic acid (31.21-41.15%), and fucose (10.90-17.63%), but PSTF had glucose (9.13%) whereas PSMF had galactose (7.40%).					
33096174	4	50	theme	structural	883:892	arg1	characteristics					894:908	similar structural characteristics	875:908	similar structural characteristics	875:908	FT-IR assay and 1H and 13C NMR spectroscopy revealed that fruits extract showed similar structural characteristics.					
33096174	10	51	theme	~80	1534:1536	arg1	%					1537:1537	%	1537:1537	%	1537:1537	PSMF highly mitigated H2O2-induced hemolysis and exhibited ~80% antihemolytic activity.					
33096174	11	52	theme	inhibitory	1607:1616	arg1	activity					1618:1625	potent inhibitory activity	1600:1625	potent inhibitory activity against AAPH-induced plasmid DNA damage	1600:1665	The extracted polysaccharides showed potent inhibitory activity against AAPH-induced plasmid DNA damage.					
33096174	1	53	dep	in	551:552	arg1	vivo					554:557	vivo	554:557	vivo	554:557	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	10	54	theme	antihemolytic	1539:1551	arg1	activity					1553:1560	~80% antihemolytic activity	1534:1560	~80% antihemolytic activity	1534:1560	PSMF highly mitigated H2O2-induced hemolysis and exhibited ~80% antihemolytic activity.					
33096174	0	55	theme	anti-genotoxicity	164:180	arg1	capacities					215:224	anti-genotoxicity, antidiabetic, and antihemolytic capacities	164:224	anti-genotoxicity, antidiabetic, and antihemolytic capacities	164:224	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	4	56	theme	fruits	853:858	arg1	extract					860:866	fruits extract	853:866	fruits extract	853:866	FT-IR assay and 1H and 13C NMR spectroscopy revealed that fruits extract showed similar structural characteristics.					
33096174	8	57	theme	highest	1383:1389	arg1	effects					1403:1409	the highest antioxidant effects	1379:1409	the highest antioxidant effects	1379:1409	PSTF exhibited the highest antioxidant effects.					
33096174	1	58	theme	monosaccharide	499:512	arg1	composition					514:524	monosaccharide composition	499:524	monosaccharide composition	499:524	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	1	58	theme	monosaccharide	499:512	arg1	structures					454:463	their structures	448:463	their structures	448:463	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	9	59	dep	in	1445:1446	arg1	vitro					1448:1452	vitro	1448:1452	vitro	1448:1452	Both of them showed a remarkable in vitro antidiabetic effect.					
33096174	1	60	theme	crude	334:338	arg1	polysaccharides					340:354	crude polysaccharides	334:354	crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L	334:422	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	8	61	theme	antioxidant	1391:1401	arg1	effects					1403:1409	the highest antioxidant effects	1379:1409	the highest antioxidant effects	1379:1409	PSTF exhibited the highest antioxidant effects.					
33096174	5	62	theme	HPLC-RID	951:958	arg1	analysis					960:967	HPLC-RID analysis	951:967	HPLC-RID analysis	951:967	Thin layer chromatography together with HPLC-RID analysis showed that the monosaccharide composition varied significantly between species.					
33096174	0	63	theme	antidiabetic	183:194	arg1	capacities					215:224	anti-genotoxicity, antidiabetic, and antihemolytic capacities	164:224	anti-genotoxicity, antidiabetic, and antihemolytic capacities	164:224	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	7	64	theme	Functional	1216:1225	arg1	analyses					1227:1234	Functional analyses	1216:1234	Functional analyses	1216:1234	Functional analyses demonstrated that samples exhibited favorable water- and oil-retention capacity, emulsifying properties, and foaming qualities.					
33096174	12	65	theme	interesting	1691:1701	arg1	capacities					1749:1758	interesting in vivo antinociceptive and anti-inflammatory capacities	1691:1758	interesting in vivo antinociceptive and anti-inflammatory capacities	1691:1758	PSTF and PSMF revealed interesting in vivo antinociceptive and anti-inflammatory capacities.					
33096174	4	66	theme	FT-IR	795:799	arg1	assay					801:805	FT-IR assay	795:805	FT-IR assay	795:805	FT-IR assay and 1H and 13C NMR spectroscopy revealed that fruits extract showed similar structural characteristics.					
33096174	11	67	theme	plasmid	1648:1654	arg1	damage					1660:1665	AAPH-induced plasmid DNA damage	1635:1665	AAPH-induced plasmid DNA damage	1635:1665	The extracted polysaccharides showed potent inhibitory activity against AAPH-induced plasmid DNA damage.					
33096174	0	68	theme	molle	87:91	arg1	fruits					93:98	Schinus molle fruits	79:98	Schinus molle fruits	79:98	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	11	69	theme	DNA	1656:1658	arg1	damage					1660:1665	AAPH-induced plasmid DNA damage	1635:1665	AAPH-induced plasmid DNA damage	1635:1665	The extracted polysaccharides showed potent inhibitory activity against AAPH-induced plasmid DNA damage.					
33096174	0	70	dep	insights	17:24	arg1	antioxidant					151:161	in vitro antioxidant	142:161	in vitro antioxidant	142:161	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	0	70	dep	insights	17:24	arg1	capacities					215:224	anti-genotoxicity, antidiabetic, and antihemolytic capacities	164:224	anti-genotoxicity, antidiabetic, and antihemolytic capacities	164:224	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	0	70	dep	insights	17:24	arg1	properties					278:287	in vivo anti-inflammatory and anti-nociceptive properties	231:287	in vivo anti-inflammatory and anti-nociceptive properties	231:287	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	0	70	dep	insights	17:24	arg1	profiles					132:139	Physicochemical and functional profiles	101:139	Physicochemical and functional profiles	101:139	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	1	71	dep	based	439:443	arg1	PSMF					426:429	PSMF	426:429	PSMF	426:429	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	0	72	theme	Schinus	79:85	arg1	fruits					93:98	Schinus molle fruits	79:98	Schinus molle fruits	79:98	Multidirectional insights on polysaccharides from Schinus terebinthifolius and Schinus molle fruits: Physicochemical and functional profiles, in vitro antioxidant, anti-genotoxicity, antidiabetic, and antihemolytic capacities, and in vivo anti-inflammatory and anti-nociceptive properties.					
33096174	3	73	theme	Remarkable	642:651	arg1	variability					653:663	Remarkable variability	642:663	Remarkable variability	642:663	Remarkable variability was detected in the content of carbohydrates (80.64 ± 0.98%), protein (1.80 ± 0.28%), fat (0.04 ± 0.005%) and ash (6.32 ± 0.26%).					
33096174	5	74	theme	Thin	911:914	arg1	chromatography					922:935	Thin layer chromatography	911:935	Thin layer chromatography together with HPLC-RID analysis	911:967	Thin layer chromatography together with HPLC-RID analysis showed that the monosaccharide composition varied significantly between species.					
33096174	1	75	dep	Raddi	396:400	arg1	L					422:422	L	422:422	L	422:422	The aim of the current study was to compare crude polysaccharides extracted from Schinus terebinthifolius Raddi (PSTF) and S. molle L. (PSMF) fruits based on their structures, physicochemical characteristics, monosaccharide composition, as well as in vitro and in vivo assays.					
33096174	4	76	theme	NMR	822:824	arg1	spectroscopy					826:837	NMR spectroscopy	822:837	NMR spectroscopy	822:837	FT-IR assay and 1H and 13C NMR spectroscopy revealed that fruits extract showed similar structural characteristics.					
33181217	6	0	from	behaviours	1015:1024	arg1	presence					886:893	the presence	882:893	the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum	882:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	6	0	from	behaviours	1015:1024	arg1	spectrum					1036:1043	the ε″ spectrum	1029:1043	the ε″ spectrum	1029:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	1	1	theme	physicochemical	176:190	arg1	properties					192:201	the dielectric and physicochemical properties	157:201	the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective	157:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	0	2	theme	composites	97:106	arg1	activity					52:59	physicochemical activity	36:59	physicochemical activity of porous hydroxyapatite/cornstarch composites	36:106	Dielectric and material analysis on physicochemical activity of porous hydroxyapatite/cornstarch composites.					
33181217	7	3	theme	composite	1128:1136	arg1	properties					1103:1112	physicochemical and dielectric properties	1072:1112	physicochemical and dielectric properties of the porous composite	1072:1136	The relationships between physicochemical and dielectric properties of the porous composite facilitate the development of the non-destructive microwave evaluation test for the porous composite.					
33181217	1	4	theme	properties	192:201	arg1	analysis					145:152	a comprehensive analysis	129:152	a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective	129:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	3	5	theme	dielectric	383:392	arg1	spectra					407:413	The dielectric permittivity spectra	379:413	The dielectric permittivity spectra	379:413	The dielectric permittivity spectra were obtained in Ku-band (12.4-18.0 GHz) and it was correlated with the physicochemical properties of the porous HAp/Cs.					
33181217	0	6	from	Dielectric	0:9	arg1	activity					52:59	physicochemical activity	36:59	physicochemical activity of porous hydroxyapatite/cornstarch composites	36:106	Dielectric and material analysis on physicochemical activity of porous hydroxyapatite/cornstarch composites.					
33181217	4	7	theme	Porous	536:541	arg1	composites					550:559	Porous HAp/Cs composites	536:559	Porous HAp/Cs composites	536:559	Porous HAp/Cs composites exhibits low ε' and negative ε″, which influenced by the microstructural morphology, interaction between Hap and Cs, as well as crystalline features due to the various proportion of the HAp/Cs.					
33181217	1	8	from	properties	192:201	arg1	perspective					272:282	a new perspective	266:282	a new perspective	266:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	3	9	theme	permittivity	394:405	arg1	spectra					407:413	The dielectric permittivity spectra	379:413	The dielectric permittivity spectra	379:413	The dielectric permittivity spectra were obtained in Ku-band (12.4-18.0 GHz) and it was correlated with the physicochemical properties of the porous HAp/Cs.					
33181217	6	10	theme	relaxation	916:925	arg1	responses					927:935	the three obvious relaxation responses	898:935	the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum	898:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	5	11	dep	polarization	826:837	arg1	the					811:813	the	811:813	the	811:813	The physicochemical effect of the composites results in the dielectric polarization and energy loss.					
33181217	3	12	theme	porous	521:526	arg1	HAp/Cs					528:533	the porous HAp/Cs	517:533	the porous HAp/Cs	517:533	The dielectric permittivity spectra were obtained in Ku-band (12.4-18.0 GHz) and it was correlated with the physicochemical properties of the porous HAp/Cs.					
33181217	7	13	theme	evaluation	1198:1207	arg1	test					1209:1212	the non-destructive microwave evaluation test	1168:1212	the non-destructive microwave evaluation test for the porous composite	1168:1237	The relationships between physicochemical and dielectric properties of the porous composite facilitate the development of the non-destructive microwave evaluation test for the porous composite.					
33181217	7	14	theme	porous	1222:1227	arg1	composite					1229:1237	the porous composite	1218:1237	the porous composite	1218:1237	The relationships between physicochemical and dielectric properties of the porous composite facilitate the development of the non-destructive microwave evaluation test for the porous composite.					
33181217	5	15	theme	composites	789:798	arg1	effect					775:780	The physicochemical effect	755:780	The physicochemical effect of the composites	755:798	The physicochemical effect of the composites results in the dielectric polarization and energy loss.					
33181217	5	16	theme	dielectric	815:824	arg1	polarization					826:837	dielectric polarization	815:837	dielectric polarization	815:837	The physicochemical effect of the composites results in the dielectric polarization and energy loss.					
33181217	6	17	theme	responses	927:935	arg1	presence					886:893	the presence	882:893	the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum	882:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	7	18	theme	dielectric	1092:1101	arg1	properties					1103:1112	physicochemical and dielectric properties	1072:1112	physicochemical and dielectric properties of the porous composite	1072:1136	The relationships between physicochemical and dielectric properties of the porous composite facilitate the development of the non-destructive microwave evaluation test for the porous composite.					
33181217	5	19	theme	physicochemical	759:773	arg1	effect					775:780	The physicochemical effect	755:780	The physicochemical effect of the composites	755:798	The physicochemical effect of the composites results in the dielectric polarization and energy loss.					
33181217	4	20	theme	HAp/Cs	747:752	arg1	proportion					729:738	the various proportion	717:738	the various proportion of the HAp/Cs	717:752	Porous HAp/Cs composites exhibits low ε' and negative ε″, which influenced by the microstructural morphology, interaction between Hap and Cs, as well as crystalline features due to the various proportion of the HAp/Cs.					
33181217	6	21	theme	obvious	908:914	arg1	responses					927:935	the three obvious relaxation responses	898:935	the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum	898:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	1	22	theme	porous	210:215	arg1	HAp/Cs					244:249	HAp/Cs	244:249	HAp/Cs	244:249	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	1	22	theme	porous	210:215	arg1	hydroxyapatite/cornstarch					217:241	porous hydroxyapatite/cornstarch	210:241	the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective	206:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	7	23	theme	test	1209:1212	arg1	development					1153:1163	the development	1149:1163	the development of the non-destructive microwave evaluation test for the porous composite	1149:1237	The relationships between physicochemical and dielectric properties of the porous composite facilitate the development of the non-destructive microwave evaluation test for the porous composite.					
33181217	4	24	theme	negative	581:588	arg1	ε″					590:591	negative ε″	581:591	negative ε″	581:591	Porous HAp/Cs composites exhibits low ε' and negative ε″, which influenced by the microstructural morphology, interaction between Hap and Cs, as well as crystalline features due to the various proportion of the HAp/Cs.					
33181217	1	25	theme	hydroxyapatite/cornstarch	217:241	arg1	composites					252:261	the porous hydroxyapatite/cornstarch (HAp/Cs) composites	206:261	the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective	206:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	7	26	theme	microwave	1188:1196	arg1	test					1209:1212	the non-destructive microwave evaluation test	1168:1212	the non-destructive microwave evaluation test for the porous composite	1168:1237	The relationships between physicochemical and dielectric properties of the porous composite facilitate the development of the non-destructive microwave evaluation test for the porous composite.					
33181217	7	27	theme	physicochemical	1072:1086	arg1	properties					1103:1112	physicochemical and dielectric properties	1072:1112	physicochemical and dielectric properties of the porous composite	1072:1136	The relationships between physicochemical and dielectric properties of the porous composite facilitate the development of the non-destructive microwave evaluation test for the porous composite.					
33181217	3	28	theme	physicochemical	487:501	arg1	properties					503:512	the physicochemical properties	483:512	the physicochemical properties of the porous HAp/Cs	483:533	The dielectric permittivity spectra were obtained in Ku-band (12.4-18.0 GHz) and it was correlated with the physicochemical properties of the porous HAp/Cs.					
33181217	0	29	theme	material	15:22	arg1	analysis					24:31	material analysis	15:31	material analysis	15:31	Dielectric and material analysis on physicochemical activity of porous hydroxyapatite/cornstarch composites.					
33181217	6	30	dep	spectrum	947:954	arg1	15.2-16.0					968:976	15.2-16.0	968:976	15.2-16.0	968:976	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	6	30	dep	spectrum	947:954	arg1	16.6-17.4 GHz					983:995	16.6-17.4 GHz	983:995	16.6-17.4 GHz	983:995	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	0	31	theme	physicochemical	36:50	arg1	activity					52:59	physicochemical activity	36:59	physicochemical activity of porous hydroxyapatite/cornstarch composites	36:106	Dielectric and material analysis on physicochemical activity of porous hydroxyapatite/cornstarch composites.					
33181217	3	32	theme	HAp/Cs	528:533	arg1	properties					503:512	the physicochemical properties	483:512	the physicochemical properties of the porous HAp/Cs	483:533	The dielectric permittivity spectra were obtained in Ku-band (12.4-18.0 GHz) and it was correlated with the physicochemical properties of the porous HAp/Cs.					
33181217	7	33	theme	non-destructive	1172:1186	arg1	test					1209:1212	the non-destructive microwave evaluation test	1168:1212	the non-destructive microwave evaluation test for the porous composite	1168:1237	The relationships between physicochemical and dielectric properties of the porous composite facilitate the development of the non-destructive microwave evaluation test for the porous composite.					
33181217	4	34	theme	low	570:572	arg1	ε					574:574	low ε	570:574	low ε	570:574	Porous HAp/Cs composites exhibits low ε' and negative ε″, which influenced by the microstructural morphology, interaction between Hap and Cs, as well as crystalline features due to the various proportion of the HAp/Cs.					
33181217	1	35	theme	composites	252:261	arg1	properties					192:201	the dielectric and physicochemical properties	157:201	the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective	157:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	6	36	from	spectrum	947:954	arg1	presence					886:893	the presence	882:893	the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum	882:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	6	36	from	spectrum	947:954	arg1	spectrum					1036:1043	the ε″ spectrum	1029:1043	the ε″ spectrum	1029:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	6	37	from	responses	927:935	arg1	behaviours					1015:1024	the negative behaviours	1002:1024	the negative behaviours in the ε″ spectrum	1002:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	6	37	from	responses	927:935	arg1	spectrum					947:954	the ε' spectrum	940:954	the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz)	940:996	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	1	38	from	composites	252:261	arg1	perspective					272:282	a new perspective	266:282	a new perspective	266:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	1	39	theme	comprehensive	131:143	arg1	analysis					145:152	a comprehensive analysis	129:152	a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective	129:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	4	40	theme	HAp/Cs	543:548	arg1	composites					550:559	Porous HAp/Cs composites	536:559	Porous HAp/Cs composites	536:559	Porous HAp/Cs composites exhibits low ε' and negative ε″, which influenced by the microstructural morphology, interaction between Hap and Cs, as well as crystalline features due to the various proportion of the HAp/Cs.					
33181217	1	41	from	perspective	272:282	arg1	properties					192:201	the dielectric and physicochemical properties	157:201	the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective	157:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	2	42	theme	dielectric	354:363	arg1	spectroscopy					365:376	dielectric spectroscopy	354:376	dielectric spectroscopy	354:376	The porous composites have been characterized via SEM, FTIR, XRD and dielectric spectroscopy.					
33181217	4	43	theme	various	721:727	arg1	proportion					729:738	the various proportion	717:738	the various proportion of the HAp/Cs	717:752	Porous HAp/Cs composites exhibits low ε' and negative ε″, which influenced by the microstructural morphology, interaction between Hap and Cs, as well as crystalline features due to the various proportion of the HAp/Cs.					
33181217	6	44	from	presence	886:893	arg1	behaviours					1015:1024	the negative behaviours	1002:1024	the negative behaviours in the ε″ spectrum	1002:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	6	44	from	presence	886:893	arg1	spectrum					947:954	the ε' spectrum	940:954	the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz)	940:996	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	2	45	theme	porous	289:294	arg1	composites					296:305	The porous composites	285:305	The porous composites	285:305	The porous composites have been characterized via SEM, FTIR, XRD and dielectric spectroscopy.					
33181217	7	46	theme	porous	1121:1126	arg1	composite					1128:1136	the porous composite	1117:1136	the porous composite	1117:1136	The relationships between physicochemical and dielectric properties of the porous composite facilitate the development of the non-destructive microwave evaluation test for the porous composite.					
33181217	4	47	theme	microstructural	618:632	arg1	morphology					634:643	the microstructural morphology	614:643	the microstructural morphology	614:643	Porous HAp/Cs composites exhibits low ε' and negative ε″, which influenced by the microstructural morphology, interaction between Hap and Cs, as well as crystalline features due to the various proportion of the HAp/Cs.					
33181217	0	48	theme	hydroxyapatite/cornstarch	71:95	arg1	composites					97:106	porous hydroxyapatite/cornstarch composites	64:106	porous hydroxyapatite/cornstarch composites	64:106	Dielectric and material analysis on physicochemical activity of porous hydroxyapatite/cornstarch composites.					
33181217	6	49	theme	ε″	1033:1034	arg1	spectrum					1036:1043	the ε″ spectrum	1029:1043	the ε″ spectrum	1029:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
33181217	1	50	theme	dielectric	161:170	arg1	properties					192:201	the dielectric and physicochemical properties	157:201	the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective	157:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	1	51	theme	new	268:270	arg1	perspective					272:282	a new perspective	266:282	a new perspective	266:282	This paper provides a comprehensive analysis of the dielectric and physicochemical properties of the porous hydroxyapatite/cornstarch (HAp/Cs) composites in a new perspective.					
33181217	0	52	theme	porous	64:69	arg1	composites					97:106	porous hydroxyapatite/cornstarch composites	64:106	porous hydroxyapatite/cornstarch composites	64:106	Dielectric and material analysis on physicochemical activity of porous hydroxyapatite/cornstarch composites.					
33181217	0	53	from	analysis	24:31	arg1	activity					52:59	physicochemical activity	36:59	physicochemical activity of porous hydroxyapatite/cornstarch composites	36:106	Dielectric and material analysis on physicochemical activity of porous hydroxyapatite/cornstarch composites.					
33181217	5	54	theme	energy	843:848	arg1	loss					850:853	energy loss	843:853	energy loss	843:853	The physicochemical effect of the composites results in the dielectric polarization and energy loss.					
33181217	4	55	theme	crystalline	689:699	arg1	features					701:708	crystalline features	689:708	low ε' and negative ε″, which influenced by the microstructural morphology, interaction between Hap and Cs, as well as crystalline features	570:708	Porous HAp/Cs composites exhibits low ε' and negative ε″, which influenced by the microstructural morphology, interaction between Hap and Cs, as well as crystalline features due to the various proportion of the HAp/Cs.					
33181217	6	56	theme	negative	1006:1013	arg1	behaviours					1015:1024	the negative behaviours	1002:1024	the negative behaviours in the ε″ spectrum	1002:1043	This phenomenon indicates the presence of the three obvious relaxation responses in the ε' spectrum (13.2-14.0, 15.2-16.0, and 16.6-17.4 GHz) and the negative behaviours in the ε″ spectrum.					
34641277	4	0	from	level	701:705	arg1	richness					664:671	the relative richness	651:671	the relative richness of Firmicutes at the phylum level	651:705	In addition, CMP-1 could increase the relative abundance of Bacteroidetes and reduce the relative richness of Firmicutes at the phylum level.					
34641277	2	1	from	NF-κB	408:412	arg1	spleen					417:422	spleen	417:422	spleen	417:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	4	2	theme	Bacteroidetes	626:638	arg1	abundance					613:621	the relative abundance	600:621	the relative abundance of Bacteroidetes	600:638	In addition, CMP-1 could increase the relative abundance of Bacteroidetes and reduce the relative richness of Firmicutes at the phylum level.					
34641277	2	3	from	p65	400:402	arg1	spleen					417:422	spleen	417:422	spleen	417:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	1	4	theme	pachymaran	179:188	arg1	types					156:160	two types	152:160	two types of carboxymethyl pachymaran (CMP-1 and CMP-2)	152:206	The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated.					
34641277	0	5	from	Activity	17:24	arg1	Mice					74:77	Immunosuppressed Mice	57:77	Immunosuppressed Mice	57:77	Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide.					
34641277	7	6	theme	functional	1113:1122	arg1	foods					1124:1128	functional foods	1113:1128	functional foods	1113:1128	The results suggested that CMP-1 and CMP-2 would be as potential immunomodulatory agents in functional foods.					
34641277	2	7	from	spleen	417:422	arg1	expressions					372:382	the mRNA expressions	363:382	the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen	363:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	4	8	theme	relative	604:611	arg1	abundance					613:621	the relative abundance	600:621	the relative abundance of Bacteroidetes	600:638	In addition, CMP-1 could increase the relative abundance of Bacteroidetes and reduce the relative richness of Firmicutes at the phylum level.					
34641277	4	9	theme	phylum	694:699	arg1	level					701:705	the phylum level	690:705	the phylum level	690:705	In addition, CMP-1 could increase the relative abundance of Bacteroidetes and reduce the relative richness of Firmicutes at the phylum level.					
34641277	4	10	theme	Firmicutes	676:685	arg1	richness					664:671	the relative richness	651:671	the relative richness of Firmicutes at the phylum level	651:705	In addition, CMP-1 could increase the relative abundance of Bacteroidetes and reduce the relative richness of Firmicutes at the phylum level.					
34641277	6	11	theme	signaling	930:938	arg1	pathway					940:946	the TLR4/NF-κB signaling pathway	915:946	the TLR4/NF-κB signaling pathway	915:946	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	3	12	theme	TNF-α	516:520	arg1	levels					506:511	the levels	502:511	the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum	502:563	CMP-1 and CMP-2 could enhance the immunity by increasing the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum.					
34641277	6	13	theme	TLR4/NF-κB	919:928	arg1	pathway					940:946	the TLR4/NF-κB signaling pathway	915:946	the TLR4/NF-κB signaling pathway	915:946	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	2	14	theme	spleen	344:349	arg1	lesions					351:357	the spleen lesions	340:357	the spleen lesions	340:357	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	3	15	from	Ig-G	551:554	arg1	serum					559:563	serum	559:563	serum	559:563	CMP-1 and CMP-2 could enhance the immunity by increasing the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum.					
34641277	7	16	theme	immunomodulatory	1086:1101	arg1	agents					1103:1108	potential immunomodulatory agents	1076:1108	potential immunomodulatory agents	1076:1108	The results suggested that CMP-1 and CMP-2 would be as potential immunomodulatory agents in functional foods.					
34641277	2	17	theme	TLR4	387:390	arg1	expressions					372:382	the mRNA expressions	363:382	the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen	363:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	6	18	dep	composition	965:975	arg1	the					961:963	the	961:963	the	961:963	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	1	19	dep	pachymaran	179:188	arg1	CMP-2					201:205	CMP-2	201:205	CMP-2	201:205	The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated.					
34641277	1	19	dep	pachymaran	179:188	arg1	CMP-1					191:195	CMP-1	191:195	CMP-1	191:195	The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated.					
34641277	0	20	theme	Immunomodulatory	0:15	arg1	Activity					17:24	Immunomodulatory Activity	0:24	Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice	0:77	Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide.					
34641277	6	21	theme	microbiota	1009:1018	arg1	abundance					981:989	abundance	981:989	abundance	981:989	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	6	21	theme	microbiota	1009:1018	arg1	composition					965:975	composition	965:975	composition	965:975	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	0	22	theme	Carboxymethyl	29:41	arg1	Pachymaran					43:52	Carboxymethyl Pachymaran	29:52	Carboxymethyl Pachymaran	29:52	Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide.					
34641277	6	23	from	expression	901:910	arg1	pathway					940:946	the TLR4/NF-κB signaling pathway	915:946	the TLR4/NF-κB signaling pathway	915:946	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	3	24	from	IFN-γ	535:539	arg1	serum					559:563	serum	559:563	serum	559:563	CMP-1 and CMP-2 could enhance the immunity by increasing the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum.					
34641277	6	25	theme	intestinal	998:1007	arg1	microbiota					1009:1018	the intestinal microbiota	994:1018	the intestinal microbiota	994:1018	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	2	26	theme	NF-κB	408:412	arg1	expressions					372:382	the mRNA expressions	363:382	the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen	363:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	1	27	theme	immunomodulatory	123:138	arg1	activity					140:147	immunomodulatory activity	123:147	immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2)	123:206	The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated.					
34641277	2	28	theme	p65	400:402	arg1	expressions					372:382	the mRNA expressions	363:382	the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen	363:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	6	29	theme	gene	896:899	arg1	expression					901:910	the gene expression	892:910	the gene expression in the TLR4/NF-κB signaling pathway	892:946	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	3	30	from	Ig-A	542:545	arg1	serum					559:563	serum	559:563	serum	559:563	CMP-1 and CMP-2 could enhance the immunity by increasing the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum.					
34641277	0	31	theme	Pachymaran	43:52	arg1	Activity					17:24	Immunomodulatory Activity	0:24	Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice	0:77	Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide.					
34641277	5	32	theme	relative	741:748	arg1	Erysipelatoclostridum					760:780	the relative abundance Erysipelatoclostridum	737:780	the relative abundance Erysipelatoclostridum at the genus level	737:799	CMP-1 and CMP-2 could reduce the relative abundance Erysipelatoclostridum at the genus level.					
34641277	2	33	theme	MyD88	393:397	arg1	expressions					372:382	the mRNA expressions	363:382	the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen	363:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	7	34	theme	potential	1076:1084	arg1	agents					1103:1108	potential immunomodulatory agents	1076:1108	potential immunomodulatory agents	1076:1108	The results suggested that CMP-1 and CMP-2 would be as potential immunomodulatory agents in functional foods.					
34641277	2	35	from	expressions	372:382	arg1	spleen					417:422	spleen	417:422	spleen	417:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	1	36	theme	activity	140:147	arg1	effects					112:118	The effects	108:118	The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice	108:245	The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated.					
34641277	0	37	theme	Immunosuppressed	57:72	arg1	Mice					74:77	Immunosuppressed Mice	57:77	Immunosuppressed Mice	57:77	Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide.					
34641277	7	38	from	agents	1103:1108	arg1	foods					1124:1128	functional foods	1113:1128	functional foods	1113:1128	The results suggested that CMP-1 and CMP-2 would be as potential immunomodulatory agents in functional foods.					
34641277	6	39	theme	mice	873:876	arg1	function					843:850	the immune function	832:850	the immune function of immunosuppression mice	832:876	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	2	40	from	TLR4	387:390	arg1	spleen					417:422	spleen	417:422	spleen	417:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	4	41	theme	relative	655:662	arg1	richness					664:671	the relative richness	651:671	the relative richness of Firmicutes at the phylum level	651:705	In addition, CMP-1 could increase the relative abundance of Bacteroidetes and reduce the relative richness of Firmicutes at the phylum level.					
34641277	3	42	from	IL-2	523:526	arg1	serum					559:563	serum	559:563	serum	559:563	CMP-1 and CMP-2 could enhance the immunity by increasing the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum.					
34641277	3	43	from	IL-6	529:532	arg1	serum					559:563	serum	559:563	serum	559:563	CMP-1 and CMP-2 could enhance the immunity by increasing the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum.					
34641277	1	44	theme	types	156:160	arg1	activity					140:147	immunomodulatory activity	123:147	immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2)	123:206	The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated.					
34641277	1	45	theme	-induced	233:240	arg1	mice					242:245	cyclophosphamide (CTX)-induced mice	211:245	cyclophosphamide (CTX)-induced mice	211:245	The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated.					
34641277	5	46	theme	genus	789:793	arg1	level					795:799	the genus level	785:799	the genus level	785:799	CMP-1 and CMP-2 could reduce the relative abundance Erysipelatoclostridum at the genus level.					
34641277	2	47	from	MyD88	393:397	arg1	spleen					417:422	spleen	417:422	spleen	417:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	2	48	theme	mRNA	367:370	arg1	expressions					372:382	the mRNA expressions	363:382	the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen	363:422	Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased.					
34641277	1	49	from	effects	112:118	arg1	mice					242:245	cyclophosphamide (CTX)-induced mice	211:245	cyclophosphamide (CTX)-induced mice	211:245	The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated.					
34641277	6	50	theme	immune	836:841	arg1	function					843:850	the immune function	832:850	the immune function of immunosuppression mice	832:876	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	6	51	theme	immunosuppression	855:871	arg1	mice					873:876	immunosuppression mice	855:876	immunosuppression mice	855:876	CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota.					
34641277	5	52	theme	abundance	750:758	arg1	Erysipelatoclostridum					760:780	the relative abundance Erysipelatoclostridum	737:780	the relative abundance Erysipelatoclostridum at the genus level	737:799	CMP-1 and CMP-2 could reduce the relative abundance Erysipelatoclostridum at the genus level.					
34641277	5	53	from	level	795:799	arg1	Erysipelatoclostridum					760:780	the relative abundance Erysipelatoclostridum	737:780	the relative abundance Erysipelatoclostridum at the genus level	737:799	CMP-1 and CMP-2 could reduce the relative abundance Erysipelatoclostridum at the genus level.					
34641277	1	54	theme	carboxymethyl	165:177	arg1	pachymaran					179:188	carboxymethyl pachymaran	165:188	carboxymethyl pachymaran (CMP-1 and CMP-2)	165:206	The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated.					
33759326	8	0	theme	other	1473:1477	arg1	plastics					1495:1502	other petroleum-based plastics	1473:1502	other petroleum-based plastics	1473:1502	Moreover, the natural degradability of the cellulose-lignin reinforced composite straws makes them promising candidates to replace plastic straws and suggests possible substitutes for other petroleum-based plastics.					
33759326	2	1	theme	water	331:335	arg1	stability					337:345	poor water stability	326:345	poor water stability	326:345	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	4	2	theme	natural	541:547	arg1	straws					600:605	all natural, degradable, cellulose-lignin reinforced composite straws	537:605	all natural, degradable, cellulose-lignin reinforced composite straws	537:605	In this paper, all natural, degradable, cellulose-lignin reinforced composite straws, inspired by the reinforcement principle of cellulose and lignin in natural wood are developed.					
33759326	0	3	theme	Reinforced	69:78	arg1	Composites					80:89	Cellulose-Lignin Reinforced Composites	52:89	Cellulose-Lignin Reinforced Composites	52:89	Strong, Hydrostable, and Degradable Straws Based on Cellulose-Lignin Reinforced Composites.					
33759326	1	4	theme	long-lasting	156:167	arg1	problem					183:189	a long-lasting environmental problem	154:189	a long-lasting environmental problem	154:189	The huge consumption of single-use plastic straws has brought a long-lasting environmental problem.					
33759326	5	5	from	°C	918:919	arg1	oven					906:909	oven	906:909	oven at 150 °C	906:919	The cellulose-lignin reinforced composite straw is fabricated by rolling up a wet film made of homogeneously mixed cellulose microfibers, cellulose nanofibers, and lignin powders, which is then baked in oven at 150 °C.					
33759326	8	6	theme	plastic	1420:1426	arg1	straws					1428:1433	plastic straws	1420:1433	plastic straws	1420:1433	Moreover, the natural degradability of the cellulose-lignin reinforced composite straws makes them promising candidates to replace plastic straws and suggests possible substitutes for other petroleum-based plastics.					
33759326	6	7	dep	melts	941:945	arg1	network					987:993	the micro-nanocellulose network	963:993	the micro-nanocellulose network	963:993	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	2	8	theme	poor	326:329	arg1	stability					337:345	poor water stability	326:345	poor water stability	326:345	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	5	9	theme	lignin	867:872	arg1	powders					874:880	lignin powders	867:880	lignin powders	867:880	The cellulose-lignin reinforced composite straw is fabricated by rolling up a wet film made of homogeneously mixed cellulose microfibers, cellulose nanofibers, and lignin powders, which is then baked in oven at 150 °C.					
33759326	2	10	dep	layer	316:320	arg1	the					296:298	the	296:298	the	296:298	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	6	11	theme	lignin	934:939	arg1	melts					941:945	lignin melts	934:945	lignin melts	934:945	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	7	12	theme	paper	1187:1191	arg1	hydrostability					1256:1269	2) high hydrostability	1248:1269	2) high hydrostability	1248:1269	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	7	12	theme	paper	1187:1191	arg1	performance					1235:1245	1) excellent mechanical performance	1211:1245	1) excellent mechanical performance	1211:1245	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	7	12	theme	paper	1187:1191	arg1	cost					1283:1286	3) low cost	1276:1286	3) low cost	1276:1286	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	7	12	theme	paper	1187:1191	arg1	straws					1193:1198	paper straws	1187:1198	paper straws	1187:1198	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	7	13	theme	excellent	1214:1222	arg1	performance					1235:1245	1) excellent mechanical performance	1211:1245	1) excellent mechanical performance	1211:1245	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	6	14	theme	mechanical	1043:1052	arg1	strength					1054:1061	mechanical strength	1043:1061	mechanical strength	1043:1061	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	2	15	theme	water-proof	300:310	arg1	layer					316:320	water-proof wax layer	300:320	water-proof wax layer	300:320	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	4	16	theme	lignin	665:670	arg1	principle					638:646	the reinforcement principle	620:646	the reinforcement principle of cellulose and lignin in natural wood	620:686	In this paper, all natural, degradable, cellulose-lignin reinforced composite straws, inspired by the reinforcement principle of cellulose and lignin in natural wood are developed.					
33759326	8	17	theme	straws	1370:1375	arg1	degradability					1311:1323	the natural degradability	1299:1323	the natural degradability of the cellulose-lignin reinforced composite straws	1299:1375	Moreover, the natural degradability of the cellulose-lignin reinforced composite straws makes them promising candidates to replace plastic straws and suggests possible substitutes for other petroleum-based plastics.					
33759326	5	18	theme	mixed	812:816	arg1	microfibers					828:838	homogeneously mixed cellulose microfibers	798:838	homogeneously mixed cellulose microfibers	798:838	The cellulose-lignin reinforced composite straw is fabricated by rolling up a wet film made of homogeneously mixed cellulose microfibers, cellulose nanofibers, and lignin powders, which is then baked in oven at 150 °C.					
33759326	8	19	theme	natural	1303:1309	arg1	degradability					1311:1323	the natural degradability	1299:1323	the natural degradability of the cellulose-lignin reinforced composite straws	1299:1375	Moreover, the natural degradability of the cellulose-lignin reinforced composite straws makes them promising candidates to replace plastic straws and suggests possible substitutes for other petroleum-based plastics.					
33759326	4	20	from	principle	638:646	arg1	wood					683:686	natural wood	675:686	natural wood	675:686	In this paper, all natural, degradable, cellulose-lignin reinforced composite straws, inspired by the reinforcement principle of cellulose and lignin in natural wood are developed.					
33759326	1	21	theme	environmental	169:181	arg1	problem					183:189	a long-lasting environmental problem	154:189	a long-lasting environmental problem	154:189	The huge consumption of single-use plastic straws has brought a long-lasting environmental problem.					
33759326	5	22	theme	reinforced	724:733	arg1	straw					745:749	The cellulose-lignin reinforced composite straw	703:749	The cellulose-lignin reinforced composite straw	703:749	The cellulose-lignin reinforced composite straw is fabricated by rolling up a wet film made of homogeneously mixed cellulose microfibers, cellulose nanofibers, and lignin powders, which is then baked in oven at 150 °C.					
33759326	3	23	theme	plastic	506:512	arg1	straws					514:519	plastic straws	506:519	plastic straws	506:519	It is therefore crucial to find a high-performing alternative to mitigate the environmental problems brought by plastic straws.					
33759326	2	24	theme	easy	358:361	arg1	delamination					363:374	the easy delamination	354:374	the easy delamination of the wax layer	354:391	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	5	25	theme	wet	781:783	arg1	film					785:788	a wet film	779:788	a wet film	779:788	The cellulose-lignin reinforced composite straw is fabricated by rolling up a wet film made of homogeneously mixed cellulose microfibers, cellulose nanofibers, and lignin powders, which is then baked in oven at 150 °C.					
33759326	8	26	theme	reinforced	1349:1358	arg1	straws					1370:1375	the cellulose-lignin reinforced composite straws	1328:1375	the cellulose-lignin reinforced composite straws	1328:1375	Moreover, the natural degradability of the cellulose-lignin reinforced composite straws makes them promising candidates to replace plastic straws and suggests possible substitutes for other petroleum-based plastics.					
33759326	5	27	theme	composite	735:743	arg1	straw					745:749	The cellulose-lignin reinforced composite straw	703:749	The cellulose-lignin reinforced composite straw	703:749	The cellulose-lignin reinforced composite straw is fabricated by rolling up a wet film made of homogeneously mixed cellulose microfibers, cellulose nanofibers, and lignin powders, which is then baked in oven at 150 °C.					
33759326	0	28	theme	Strong	0:5	arg1	Straws					36:41	Strong, Hydrostable, and Degradable Straws	0:41	Strong, Hydrostable, and Degradable Straws	0:41	Strong, Hydrostable, and Degradable Straws Based on Cellulose-Lignin Reinforced Composites.					
33759326	6	29	theme	polyphenolic	1008:1019	arg1	binder					1021:1026	a polyphenolic binder	1006:1026	a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw	1006:1115	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	7	30	theme	obtained	1122:1129	arg1	straws					1131:1136	The obtained straws	1118:1136	The obtained straws	1118:1136	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	6	31	dep	strength	1054:1061	arg1	the					1039:1041	the	1039:1041	the	1039:1041	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	1	32	theme	huge	96:99	arg1	consumption					101:111	The huge consumption	92:111	The huge consumption of single-use plastic straws	92:140	The huge consumption of single-use plastic straws has brought a long-lasting environmental problem.					
33759326	8	33	theme	petroleum-based	1479:1493	arg1	plastics					1495:1502	other petroleum-based plastics	1473:1502	other petroleum-based plastics	1473:1502	Moreover, the natural degradability of the cellulose-lignin reinforced composite straws makes them promising candidates to replace plastic straws and suggests possible substitutes for other petroleum-based plastics.					
33759326	2	34	theme	layer	387:391	arg1	delamination					363:374	the easy delamination	354:374	the easy delamination of the wax layer	354:391	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	0	35	theme	Hydrostable	8:18	arg1	Straws					36:41	Strong, Hydrostable, and Degradable Straws	0:41	Strong, Hydrostable, and Degradable Straws	0:41	Strong, Hydrostable, and Degradable Straws Based on Cellulose-Lignin Reinforced Composites.					
33759326	2	36	theme	Paper	192:196	arg1	replacement					218:228	the current replacement	206:228	the current replacement for plastic straws	206:247	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	2	36	theme	Paper	192:196	arg1	straws					198:203	Paper straws	192:203	Paper straws	192:203	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	2	37	theme	high	283:286	arg1	cost					288:291	a high cost	281:291	a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer	281:391	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	2	38	theme	wax	383:385	arg1	layer					387:391	the wax layer	379:391	the wax layer	379:391	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	0	39	theme	Degradable	25:34	arg1	Straws					36:41	Strong, Hydrostable, and Degradable Straws	0:41	Strong, Hydrostable, and Degradable Straws	0:41	Strong, Hydrostable, and Degradable Straws Based on Cellulose-Lignin Reinforced Composites.					
33759326	2	40	theme	layer	316:320	arg1	cost					288:291	a high cost	281:291	a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer	281:391	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	7	41	theme	advantageous	1158:1169	arg1	properties					1171:1180	several advantageous properties	1150:1180	several advantageous properties	1150:1180	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	7	42	theme	mechanical	1224:1233	arg1	performance					1235:1245	1) excellent mechanical performance	1211:1245	1) excellent mechanical performance	1211:1245	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	8	43	theme	composite	1360:1368	arg1	straws					1370:1375	the cellulose-lignin reinforced composite straws	1328:1375	the cellulose-lignin reinforced composite straws	1328:1375	Moreover, the natural degradability of the cellulose-lignin reinforced composite straws makes them promising candidates to replace plastic straws and suggests possible substitutes for other petroleum-based plastics.					
33759326	7	44	theme	low	1279:1281	arg1	cost					1283:1286	3) low cost	1276:1286	3) low cost	1276:1286	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	5	45	theme	cellulose	818:826	arg1	microfibers					828:838	homogeneously mixed cellulose microfibers	798:838	homogeneously mixed cellulose microfibers	798:838	The cellulose-lignin reinforced composite straw is fabricated by rolling up a wet film made of homogeneously mixed cellulose microfibers, cellulose nanofibers, and lignin powders, which is then baked in oven at 150 °C.					
33759326	4	46	theme	reinforcement	624:636	arg1	principle					638:646	the reinforcement principle	620:646	the reinforcement principle of cellulose and lignin in natural wood	620:686	In this paper, all natural, degradable, cellulose-lignin reinforced composite straws, inspired by the reinforcement principle of cellulose and lignin in natural wood are developed.					
33759326	4	47	dep	natural	541:547	arg1	degradable					550:559	degradable	550:559	degradable	550:559	In this paper, all natural, degradable, cellulose-lignin reinforced composite straws, inspired by the reinforcement principle of cellulose and lignin in natural wood are developed.					
33759326	4	47	dep	natural	541:547	arg1	reinforced					579:588	reinforced	579:588	reinforced	579:588	In this paper, all natural, degradable, cellulose-lignin reinforced composite straws, inspired by the reinforcement principle of cellulose and lignin in natural wood are developed.					
33759326	2	48	theme	plastic	234:240	arg1	straws					242:247	plastic straws	234:247	plastic straws	234:247	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	7	49	dep	cost	1283:1286	arg1	3					1276:1276	3	1276:1276	3	1276:1276	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	3	50	theme	high-performing	428:442	arg1	alternative					444:454	a high-performing alternative	426:454	a high-performing alternative to mitigate the environmental problems brought by plastic straws	426:519	It is therefore crucial to find a high-performing alternative to mitigate the environmental problems brought by plastic straws.					
33759326	2	51	theme	current	210:216	arg1	replacement					218:228	the current replacement	206:228	the current replacement for plastic straws	206:247	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	2	51	theme	current	210:216	arg1	straws					198:203	Paper straws	192:203	Paper straws	192:203	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	6	52	theme	straw	1111:1115	arg1	strength					1054:1061	mechanical strength	1043:1061	mechanical strength	1043:1061	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	6	52	theme	straw	1111:1115	arg1	performance					1082:1092	hydrophobicity performance	1067:1092	hydrophobicity performance	1067:1092	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	3	53	theme	environmental	472:484	arg1	problems					486:493	the environmental problems	468:493	the environmental problems brought by plastic straws	468:519	It is therefore crucial to find a high-performing alternative to mitigate the environmental problems brought by plastic straws.					
33759326	1	54	theme	single-use	116:125	arg1	straws					135:140	single-use plastic straws	116:140	single-use plastic straws	116:140	The huge consumption of single-use plastic straws has brought a long-lasting environmental problem.					
33759326	6	55	theme	micro-nanocellulose	967:985	arg1	network					987:993	the micro-nanocellulose network	963:993	the micro-nanocellulose network	963:993	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	8	56	theme	promising	1388:1396	arg1	candidates					1398:1407	promising candidates	1388:1407	promising candidates	1388:1407	Moreover, the natural degradability of the cellulose-lignin reinforced composite straws makes them promising candidates to replace plastic straws and suggests possible substitutes for other petroleum-based plastics.					
33759326	7	57	dep	hydrostability	1256:1269	arg1	2					1248:1248	2	1248:1248	2	1248:1248	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	4	58	theme	cellulose	651:659	arg1	principle					638:646	the reinforcement principle	620:646	the reinforcement principle of cellulose and lignin in natural wood	620:686	In this paper, all natural, degradable, cellulose-lignin reinforced composite straws, inspired by the reinforcement principle of cellulose and lignin in natural wood are developed.					
33759326	1	59	theme	plastic	127:133	arg1	straws					135:140	single-use plastic straws	116:140	single-use plastic straws	116:140	The huge consumption of single-use plastic straws has brought a long-lasting environmental problem.					
33759326	6	60	theme	hydrophobicity	1067:1080	arg1	performance					1082:1092	hydrophobicity performance	1067:1092	hydrophobicity performance	1067:1092	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	5	61	theme	cellulose-lignin	707:722	arg1	straw					745:749	The cellulose-lignin reinforced composite straw	703:749	The cellulose-lignin reinforced composite straw	703:749	The cellulose-lignin reinforced composite straw is fabricated by rolling up a wet film made of homogeneously mixed cellulose microfibers, cellulose nanofibers, and lignin powders, which is then baked in oven at 150 °C.					
33759326	6	62	dep	baked	927:931	arg1	melts					941:945	lignin melts	934:945	lignin melts	934:945	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	6	62	dep	baked	927:931	arg1	infiltrates					951:961	infiltrates	951:961	infiltrates	951:961	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	1	63	theme	straws	135:140	arg1	consumption					101:111	The huge consumption	92:111	The huge consumption of single-use plastic straws	92:140	The huge consumption of single-use plastic straws has brought a long-lasting environmental problem.					
33759326	2	64	theme	wax	312:314	arg1	layer					316:320	water-proof wax layer	300:320	water-proof wax layer	300:320	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	0	65	theme	Cellulose-Lignin	52:67	arg1	Composites					80:89	Cellulose-Lignin Reinforced Composites	52:89	Cellulose-Lignin Reinforced Composites	52:89	Strong, Hydrostable, and Degradable Straws Based on Cellulose-Lignin Reinforced Composites.					
33759326	8	66	theme	cellulose-lignin	1332:1347	arg1	straws					1370:1375	the cellulose-lignin reinforced composite straws	1328:1375	the cellulose-lignin reinforced composite straws	1328:1375	Moreover, the natural degradability of the cellulose-lignin reinforced composite straws makes them promising candidates to replace plastic straws and suggests possible substitutes for other petroleum-based plastics.					
33759326	2	67	theme	due	347:349	arg1	layer					316:320	water-proof wax layer	300:320	water-proof wax layer	300:320	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	5	68	theme	cellulose	841:849	arg1	nanofibers					851:860	cellulose nanofibers	841:860	cellulose nanofibers	841:860	The cellulose-lignin reinforced composite straw is fabricated by rolling up a wet film made of homogeneously mixed cellulose microfibers, cellulose nanofibers, and lignin powders, which is then baked in oven at 150 °C.					
33759326	4	69	theme	composite	590:598	arg1	straws					600:605	all natural, degradable, cellulose-lignin reinforced composite straws	537:605	all natural, degradable, cellulose-lignin reinforced composite straws	537:605	In this paper, all natural, degradable, cellulose-lignin reinforced composite straws, inspired by the reinforcement principle of cellulose and lignin in natural wood are developed.					
33759326	6	70	theme	resulting	1101:1109	arg1	straw					1111:1115	the resulting straw	1097:1115	the resulting straw	1097:1115	When baked, lignin melts and infiltrates the micro-nanocellulose network, acting as a polyphenolic binder to improve the mechanical strength and hydrophobicity performance of the resulting straw.					
33759326	7	71	theme	high	1251:1254	arg1	hydrostability					1256:1269	2) high hydrostability	1248:1269	2) high hydrostability	1248:1269	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	2	72	theme	stability	337:345	arg1	cost					288:291	a high cost	281:291	a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer	281:391	Paper straws, the current replacement for plastic straws, suffer from drawbacks, such as a high cost of the water-proof wax layer and poor water stability due to the easy delamination of the wax layer.					
33759326	7	73	theme	several	1150:1156	arg1	properties					1171:1180	several advantageous properties	1150:1180	several advantageous properties	1150:1180	The obtained straws demonstrate several advantageous properties over paper straws, including 1) excellent mechanical performance, 2) high hydrostability, and 3) low cost.					
33759326	4	74	theme	natural	675:681	arg1	wood					683:686	natural wood	675:686	natural wood	675:686	In this paper, all natural, degradable, cellulose-lignin reinforced composite straws, inspired by the reinforcement principle of cellulose and lignin in natural wood are developed.					
32507202	2	0	theme	-β-d-Glcp-	516:525	arg1	-3-O-acetyl-β-d-Manp-					584:604	→4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→	513:607	-3-O-acetyl-β-d-Manp-	584:604	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	3	1	theme	significant	682:692	arg1	effects					707:713	significant hypoglycemic effects	682:713	significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats	682:833	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	4	2	theme	GLP-1	889:893	arg1	secretion					895:903	DOP-induced GLP-1 secretion	877:903	DOP-induced GLP-1 secretion	877:903	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	2	3	theme	methylation	396:406	arg1	analyses					440:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses	327:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses	327:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	4	4	theme	DOP-induced	877:887	arg1	secretion					895:903	DOP-induced GLP-1 secretion	877:903	DOP-induced GLP-1 secretion	877:903	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	5	theme	kinases	1105:1111	arg1	inhibitor					1120:1128	a p38-mitogen-activated protein kinases (MAPK) inhibitor	1073:1128	a p38-mitogen-activated protein kinases (MAPK) inhibitor	1073:1128	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	2	6	theme	-β-d-Manp-	534:543	arg1	-3-O-acetyl-β-d-Manp-					584:604	→4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→	513:607	-3-O-acetyl-β-d-Manp-	584:604	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	4	7	theme	protein	1097:1103	arg1	MAPK					1114:1117	MAPK	1114:1117	MAPK	1114:1117	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	7	theme	protein	1097:1103	arg1	kinases					1105:1111	p38-mitogen-activated protein kinases	1075:1111	a p38-mitogen-activated protein kinases (MAPK) inhibitor	1073:1128	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	5	8	theme	sugar	1192:1196	arg1	levels					1198:1203	fasting blood sugar levels	1178:1203	fasting blood sugar levels	1178:1203	These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.					
32507202	0	9	theme	polysaccharides	109:123	arg1	secretion					92:100	glucagon-like peptide-1 secretion	68:100	glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale	68:150	Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale.					
32507202	2	10	contain	have	484:487	arg1	DOP-2					474:478	DOP-2	474:478	DOP-2	474:478	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	2	10	contain	have	484:487	arg1	DOP-1					464:468	DOP-1	464:468	DOP-1	464:468	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	2	10	contain	have	484:487	arg2	backbone					491:498	a backbone	489:498	a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→	489:607	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	5	11	theme	DOP-induced	1259:1269	arg1	secretion					1277:1285	intracellular DOP-induced GLP-1 secretion	1245:1285	intracellular DOP-induced GLP-1 secretion	1245:1285	These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.					
32507202	3	12	theme	increased	728:736	arg1	levels					788:793	increased serum insulin and glucagon-like peptide-1 (GLP-1) levels	728:793	increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats	728:833	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	1	13	with	polysaccharides	157:171	arg1	weights					212:218	molecular weights	202:218	molecular weights of 6.8 kDa and 14.3 kDa	202:242	Two polysaccharides, named DOP-1 and DOP-2, with molecular weights of 6.8 kDa and 14.3 kDa, respectively, were isolated and purified from the stems of Dendrobium officinale.					
32507202	2	14	theme	magnetic	421:428	arg1	resonance					430:438	nuclear magnetic resonance	413:438	nuclear magnetic resonance	413:438	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	0	15	theme	Dendrobium	130:139	arg1	officinale					141:150	Dendrobium officinale	130:150	Dendrobium officinale	130:150	Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale.					
32507202	3	16	theme	hypoglycemic	694:705	arg1	effects					707:713	significant hypoglycemic effects	682:713	significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats	682:833	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	4	17	theme	receptor	1048:1055	arg1	antagonist					1057:1066	a specific calcium-sensing receptor antagonist	1021:1066	a specific calcium-sensing receptor antagonist	1021:1066	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	17	theme	receptor	1048:1055	arg1	inhibitor					1010:1018	a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor	957:1018	a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor	957:1018	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	5	18	theme	GLP-1	1271:1275	arg1	secretion					1277:1285	intracellular DOP-induced GLP-1 secretion	1245:1285	intracellular DOP-induced GLP-1 secretion	1245:1285	These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.					
32507202	2	19	theme	resonance	430:438	arg1	analyses					440:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses	327:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses	327:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	3	20	theme	insulin	744:750	arg1	levels					788:793	increased serum insulin and glucagon-like peptide-1 (GLP-1) levels	728:793	increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats	728:833	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	5	21	theme	GLP-1	1220:1224	arg1	secretion					1226:1234	GLP-1 secretion	1220:1234	GLP-1 secretion	1220:1234	These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.					
32507202	2	22	theme	composition	342:352	arg1	analyses					440:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses	327:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses	327:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	1	23	theme	molecular	202:210	arg1	weights					212:218	molecular weights	202:218	molecular weights of 6.8 kDa and 14.3 kDa	202:242	Two polysaccharides, named DOP-1 and DOP-2, with molecular weights of 6.8 kDa and 14.3 kDa, respectively, were isolated and purified from the stems of Dendrobium officinale.					
32507202	0	24	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale.					
32507202	4	25	theme	calcium-sensing	1032:1046	arg1	antagonist					1057:1066	a specific calcium-sensing receptor antagonist	1021:1066	a specific calcium-sensing receptor antagonist	1021:1066	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	25	theme	calcium-sensing	1032:1046	arg1	inhibitor					1010:1018	a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor	957:1018	a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor	957:1018	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	2	26	theme	Monosaccharide	327:340	arg1	composition					342:352	Monosaccharide composition	327:352	Monosaccharide composition	327:352	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	2	27	theme	nuclear	413:419	arg1	resonance					430:438	nuclear magnetic resonance	413:438	nuclear magnetic resonance	413:438	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	0	28	theme	hypoglycemic	32:43	arg1	effect					45:50	hypoglycemic effect	32:50	hypoglycemic effect	32:50	Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale.					
32507202	3	29	theme	glucagon-like	756:768	arg1	GLP-1					781:785	GLP-1	781:785	GLP-1	781:785	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	3	29	theme	glucagon-like	756:768	arg1	peptide-1					770:778	glucagon-like peptide-1	756:778	glucagon-like peptide-1 (GLP-1)	756:786	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	4	30	theme	Further	836:842	arg1	experiments					853:863	Further in vitro experiments	836:863	Further in vitro experiments	836:863	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	31	dep	in	844:845	arg1	vitro					847:851	vitro	847:851	vitro	847:851	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	3	32	theme	serum	738:742	arg1	insulin					744:750	serum insulin	738:750	serum insulin	738:750	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	4	33	theme	calcium	939:945	arg1	chelator					947:954	an intracellular calcium chelator	922:954	an intracellular calcium chelator	922:954	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	1	34	theme	6.8 kDa	223:229	arg1	weights					212:218	molecular weights	202:218	molecular weights of 6.8 kDa and 14.3 kDa	202:242	Two polysaccharides, named DOP-1 and DOP-2, with molecular weights of 6.8 kDa and 14.3 kDa, respectively, were isolated and purified from the stems of Dendrobium officinale.					
32507202	1	35	theme	Dendrobium	304:313	arg1	officinale					315:324	Dendrobium officinale	304:324	Dendrobium officinale	304:324	Two polysaccharides, named DOP-1 and DOP-2, with molecular weights of 6.8 kDa and 14.3 kDa, respectively, were isolated and purified from the stems of Dendrobium officinale.					
32507202	2	36	theme	1→	545:546	arg1	-3-O-acetyl-β-d-Manp-					584:604	→4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→	513:607	-3-O-acetyl-β-d-Manp-	584:604	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	5	37	theme	MAPK	1327:1330	arg1	pathways					1332:1339	the Ca2+/calmodulin/CaMKII and MAPK pathways	1296:1339	pathways	1332:1339	These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.					
32507202	1	38	theme	officinale	315:324	arg1	stems					295:299	the stems	291:299	the stems of Dendrobium officinale	291:324	Two polysaccharides, named DOP-1 and DOP-2, with molecular weights of 6.8 kDa and 14.3 kDa, respectively, were isolated and purified from the stems of Dendrobium officinale.					
32507202	0	39	from	secretion	92:100	arg1	officinale					141:150	Dendrobium officinale	130:150	Dendrobium officinale	130:150	Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale.					
32507202	1	40	theme	14.3 kDa	235:242	arg1	weights					212:218	molecular weights	202:218	molecular weights of 6.8 kDa and 14.3 kDa	202:242	Two polysaccharides, named DOP-1 and DOP-2, with molecular weights of 6.8 kDa and 14.3 kDa, respectively, were isolated and purified from the stems of Dendrobium officinale.					
32507202	0	41	theme	glucagon-like	68:80	arg1	secretion					92:100	glucagon-like peptide-1 secretion	68:100	glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale	68:150	Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale.					
32507202	3	42	dep	In	610:611	arg1	vivo					613:616	vivo	613:616	vivo	613:616	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	3	43	from	levels	788:793	arg1	rats					830:833	streptozotocin-induced diabetic rats	798:833	streptozotocin-induced diabetic rats	798:833	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	4	44	theme	in	844:845	arg1	experiments					853:863	Further in vitro experiments	836:863	Further in vitro experiments	836:863	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	45	theme	intracellular	925:937	arg1	chelator					947:954	an intracellular calcium chelator	922:954	an intracellular calcium chelator	922:954	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	2	46	theme	spectroscopy	382:393	arg1	analyses					440:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses	327:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses	327:447	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	4	47	theme	specific	1023:1030	arg1	antagonist					1057:1066	a specific calcium-sensing receptor antagonist	1021:1066	a specific calcium-sensing receptor antagonist	1021:1066	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	47	theme	specific	1023:1030	arg1	inhibitor					1010:1018	a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor	957:1018	a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor	957:1018	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	3	48	theme	peptide-1	770:778	arg1	levels					788:793	increased serum insulin and glucagon-like peptide-1 (GLP-1) levels	728:793	increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats	728:833	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	0	49	from	officinale	141:150	arg1	secretion					92:100	glucagon-like peptide-1 secretion	68:100	glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale	68:150	Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale.					
32507202	0	49	from	officinale	141:150	arg1	polysaccharides					109:123	two polysaccharides	105:123	two polysaccharides from Dendrobium officinale	105:150	Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale.					
32507202	4	50	theme	protein	985:991	arg1	kinase					993:998	a Ca2+/calmodulin-dependent protein kinase	957:998	a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor	957:1018	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	50	theme	protein	985:991	arg1	CaMK					1001:1004	CaMK	1001:1004	CaMK	1001:1004	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	2	51	dep	-3-O-acetyl-β-d-Manp-	584:604	arg1	→4					531:532	→4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→	513:607	→4	531:532	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	2	51	dep	-3-O-acetyl-β-d-Manp-	584:604	arg1	→4					549:550	→4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→	513:607	→4	549:550	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	2	51	dep	-3-O-acetyl-β-d-Manp-	584:604	arg1	→4					581:582	→4	581:582	→4	581:582	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	2	51	dep	-3-O-acetyl-β-d-Manp-	584:604	arg1	1→					574:575	1→	574:575	1→	574:575	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	2	52	theme	infrared	373:380	arg1	spectroscopy					382:393	Fourier-transform infrared spectroscopy	355:393	Fourier-transform infrared spectroscopy	355:393	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	5	53	theme	fasting	1178:1184	arg1	levels					1198:1203	fasting blood sugar levels	1178:1203	fasting blood sugar levels	1178:1203	These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.					
32507202	2	54	theme	-2-O-acetyl-β-d-Manp-	552:572	arg1	-3-O-acetyl-β-d-Manp-					584:604	→4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→	513:607	-3-O-acetyl-β-d-Manp-	584:604	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	5	55	theme	intracellular	1245:1257	arg1	secretion					1277:1285	intracellular DOP-induced GLP-1 secretion	1245:1285	intracellular DOP-induced GLP-1 secretion	1245:1285	These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.					
32507202	2	56	theme	Fourier-transform	355:371	arg1	spectroscopy					382:393	Fourier-transform infrared spectroscopy	355:393	Fourier-transform infrared spectroscopy	355:393	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	3	57	theme	streptozotocin-induced	798:819	arg1	rats					830:833	streptozotocin-induced diabetic rats	798:833	streptozotocin-induced diabetic rats	798:833	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	3	58	theme	D.	637:638	arg1	DOPs					668:671	DOPs	668:671	DOPs	668:671	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	3	58	theme	D.	637:638	arg1	polysaccharides					651:665	D. officinale polysaccharides	637:665	D. officinale polysaccharides (DOPs)	637:672	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	3	59	theme	In	610:611	arg1	assays					618:623	In vivo assays	610:623	In vivo assays	610:623	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	3	60	theme	diabetic	821:828	arg1	rats					830:833	streptozotocin-induced diabetic rats	798:833	streptozotocin-induced diabetic rats	798:833	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	2	61	theme	1→	527:528	arg1	-3-O-acetyl-β-d-Manp-					584:604	→4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→	513:607	-3-O-acetyl-β-d-Manp-	584:604	Monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses indicated that DOP-1 and DOP-2 may have a backbone consisted of →4)-β-d-Glcp-(1→, →4)-β-d-Manp-(1→, →4)-2-O-acetyl-β-d-Manp-(1→ and →4)-3-O-acetyl-β-d-Manp-(1→.					
32507202	3	62	theme	officinale	640:649	arg1	DOPs					668:671	DOPs	668:671	DOPs	668:671	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	3	62	theme	officinale	640:649	arg1	polysaccharides					651:665	D. officinale polysaccharides	637:665	D. officinale polysaccharides (DOPs)	637:672	In vivo assays showed that D. officinale polysaccharides (DOPs) exerted significant hypoglycemic effects accompanying increased serum insulin and glucagon-like peptide-1 (GLP-1) levels in streptozotocin-induced diabetic rats.					
32507202	5	63	theme	Ca2+/calmodulin/CaMKII	1300:1321	arg1	pathways					1332:1339	the Ca2+/calmodulin/CaMKII and MAPK pathways	1296:1339	pathways	1332:1339	These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.					
32507202	1	64	theme	named	174:178	arg1	polysaccharides					157:171	Two polysaccharides	153:171	Two polysaccharides	153:171	Two polysaccharides, named DOP-1 and DOP-2, with molecular weights of 6.8 kDa and 14.3 kDa, respectively, were isolated and purified from the stems of Dendrobium officinale.					
32507202	1	64	theme	named	174:178	arg1	DOP-1					180:184	named DOP-1	174:184	named DOP-1	174:184	Two polysaccharides, named DOP-1 and DOP-2, with molecular weights of 6.8 kDa and 14.3 kDa, respectively, were isolated and purified from the stems of Dendrobium officinale.					
32507202	0	65	theme	peptide-1	82:90	arg1	secretion					92:100	glucagon-like peptide-1 secretion	68:100	glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale	68:150	Structural characterization and hypoglycemic effect via stimulating glucagon-like peptide-1 secretion of two polysaccharides from Dendrobium officinale.					
32507202	5	66	theme	blood	1186:1190	arg1	levels					1198:1203	fasting blood sugar levels	1178:1203	fasting blood sugar levels	1178:1203	These results indicated that DOPs may decrease fasting blood sugar levels by stimulating GLP-1 secretion and that intracellular DOP-induced GLP-1 secretion involved the Ca2+/calmodulin/CaMKII and MAPK pathways.					
32507202	4	67	theme	kinase	993:998	arg1	antagonist					1057:1066	a specific calcium-sensing receptor antagonist	1021:1066	a specific calcium-sensing receptor antagonist	1021:1066	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	67	theme	kinase	993:998	arg1	inhibitor					1010:1018	a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor	957:1018	a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor	957:1018	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	68	theme	p38-mitogen-activated	1075:1095	arg1	MAPK					1114:1117	MAPK	1114:1117	MAPK	1114:1117	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	68	theme	p38-mitogen-activated	1075:1095	arg1	kinases					1105:1111	p38-mitogen-activated protein kinases	1075:1111	a p38-mitogen-activated protein kinases (MAPK) inhibitor	1073:1128	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	69	theme	Ca2+/calmodulin-dependent	959:983	arg1	kinase					993:998	a Ca2+/calmodulin-dependent protein kinase	957:998	a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor	957:1018	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
32507202	4	69	theme	Ca2+/calmodulin-dependent	959:983	arg1	CaMK					1001:1004	CaMK	1001:1004	CaMK	1001:1004	Further in vitro experiments showed that DOP-induced GLP-1 secretion was inhibited by an intracellular calcium chelator, a Ca2+/calmodulin-dependent protein kinase (CaMK) II inhibitor, a specific calcium-sensing receptor antagonist, and a p38-mitogen-activated protein kinases (MAPK) inhibitor.					
34499479	8	0	theme	whole	1498:1502	arg1	process					1504:1510	the whole process	1494:1510	the whole process of nanoparticle fabrication and fluorescent testing	1494:1562	Notably, the whole process of nanoparticle fabrication and fluorescent testing is facile and the results are highly reliable.					
34499479	8	0	theme	whole	1498:1502	arg1	facile					1567:1572	facile	1567:1572	facile	1567:1572	Notably, the whole process of nanoparticle fabrication and fluorescent testing is facile and the results are highly reliable.					
34499479	2	1	theme	rapid	334:338	arg1	growth					340:345	the rapid growth	330:345	the rapid growth of the biomedical field	330:369	This situation is becoming worse over time due to the rapid growth of the biomedical field, and thus there is an urgent need for a more effective strategy to address the problem.					
34499479	0	2	theme	Line	70:73	arg1	Identification					75:88	Cell Line Identification	65:88	Cell Line Identification	65:88	Cell Coding Arrays Based on Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation.					
34499479	5	3	theme	different	1136:1144	arg1	regions					1146:1152	different regions	1136:1152	different regions	1136:1152	Through flow cytometry measurements, the specific signals of each cell sample can be recorded and turned into a map divided into different regions by statistical processing.					
34499479	4	4	theme	cell	851:854	arg1	lines					856:860	various cell lines	843:860	various cell lines	843:860	The different affinity between each nanoparticle and various cell lines results in clearly distinguishable differences in their endocytosis degrees, thus resulting in distinct characteristic fluorescence intensities.					
34499479	1	5	theme	large	153:157	arg1	scale					159:163	a large scale	151:163	a large scale in the field of biomedicine	151:191	Cell lines are applied on a large scale in the field of biomedicine, but they are susceptible to issues such as misidentification and cross-contamination.					
34499479	0	6	theme	Cell	94:97	arg1	Contamination					99:111	Cell Contamination	94:111	Cell Contamination	94:111	Cell Coding Arrays Based on Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation.					
34499479	3	7	theme	GISA	655:658	arg1	method					661:666	the graft-copolymerization-induced self-assembly (GISA) method	605:666	the graft-copolymerization-induced self-assembly (GISA) method	605:666	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	6	8	theme	normal	1284:1289	arg1	type					1291:1294	one normal type	1280:1294	one normal type	1280:1294	Using this sensing array strategy, we have successfully identified six human cell lines, including one normal type and five tumor types.					
34499479	7	9	theme	lines	1376:1380	arg1	evaluation					1347:1356	cell contamination evaluation	1328:1356	cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis	1328:1446	Moreover, cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis has also been successfully achieved.					
34499479	3	10	theme	cell	482:485	arg1	method					494:499	a cell coding method	480:499	a cell coding method	480:499	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	5	11	theme	flow	1015:1018	arg1	measurements					1030:1041	flow cytometry measurements	1015:1041	flow cytometry measurements	1015:1041	Through flow cytometry measurements, the specific signals of each cell sample can be recorded and turned into a map divided into different regions by statistical processing.					
34499479	6	12	theme	sensing	1192:1198	arg1	strategy					1206:1213	this sensing array strategy	1187:1213	this sensing array strategy	1187:1213	Using this sensing array strategy, we have successfully identified six human cell lines, including one normal type and five tumor types.					
34499479	3	13	with	modification	740:751	arg1	molecules					779:787	fluorescent labeling molecules	758:787	fluorescent labeling molecules	758:787	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	3	14	theme	coding	487:492	arg1	method					494:499	a cell coding method	480:499	a cell coding method	480:499	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	4	15	theme	distinct	957:964	arg1	intensities					994:1004	distinct characteristic fluorescence intensities	957:1004	distinct characteristic fluorescence intensities	957:1004	The different affinity between each nanoparticle and various cell lines results in clearly distinguishable differences in their endocytosis degrees, thus resulting in distinct characteristic fluorescence intensities.					
34499479	8	16	theme	fabrication	1528:1538	arg1	process					1504:1510	the whole process	1494:1510	the whole process of nanoparticle fabrication and fluorescent testing	1494:1562	Notably, the whole process of nanoparticle fabrication and fluorescent testing is facile and the results are highly reliable.					
34499479	8	16	theme	fabrication	1528:1538	arg1	facile					1567:1572	facile	1567:1572	facile	1567:1572	Notably, the whole process of nanoparticle fabrication and fluorescent testing is facile and the results are highly reliable.					
34499479	7	17	theme	cell	1328:1331	arg1	contamination					1333:1345	cell contamination	1328:1345	cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis	1328:1446	Moreover, cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis has also been successfully achieved.					
34499479	3	18	dep	modification	740:751	arg1	elaborate					730:738	elaborate	730:738	elaborate	730:738	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	7	19	theme	cell	1371:1374	arg1	lines					1376:1380	different cell lines	1361:1380	different cell lines with HeLa cells	1361:1396	Moreover, cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis has also been successfully achieved.					
34499479	2	20	theme	field	365:369	arg1	growth					340:345	the rapid growth	330:345	the rapid growth of the biomedical field	330:369	This situation is becoming worse over time due to the rapid growth of the biomedical field, and thus there is an urgent need for a more effective strategy to address the problem.					
34499479	0	21	theme	Coding	5:10	arg1	Arrays					12:17	Cell Coding Arrays	0:17	Cell Coding Arrays	0:17	Cell Coding Arrays Based on Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation.					
34499479	8	22	theme	fluorescent	1544:1554	arg1	testing					1556:1562	fluorescent testing	1544:1562	fluorescent testing	1544:1562	Notably, the whole process of nanoparticle fabrication and fluorescent testing is facile and the results are highly reliable.					
34499479	4	23	theme	various	843:849	arg1	lines					856:860	various cell lines	843:860	various cell lines	843:860	The different affinity between each nanoparticle and various cell lines results in clearly distinguishable differences in their endocytosis degrees, thus resulting in distinct characteristic fluorescence intensities.					
34499479	4	24	theme	fluorescence	981:992	arg1	intensities					994:1004	distinct characteristic fluorescence intensities	957:1004	distinct characteristic fluorescence intensities	957:1004	The different affinity between each nanoparticle and various cell lines results in clearly distinguishable differences in their endocytosis degrees, thus resulting in distinct characteristic fluorescence intensities.					
34499479	0	25	dep	Identification	75:88	arg1	Evaluation					113:122	Evaluation	113:122	Evaluation	113:122	Cell Coding Arrays Based on Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation.					
34499479	7	26	with	lines	1376:1380	arg1	cells					1392:1396	HeLa cells	1387:1396	HeLa cells	1387:1396	Moreover, cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis has also been successfully achieved.					
34499479	2	27	theme	biomedical	354:363	arg1	field					365:369	the biomedical field	350:369	the biomedical field	350:369	This situation is becoming worse over time due to the rapid growth of the biomedical field, and thus there is an urgent need for a more effective strategy to address the problem.					
34499479	0	28	theme	Cell	0:3	arg1	Arrays					12:17	Cell Coding Arrays	0:17	Cell Coding Arrays	0:17	Cell Coding Arrays Based on Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation.					
34499479	6	29	theme	cell	1258:1261	arg1	type					1291:1294	one normal type	1280:1294	one normal type	1280:1294	Using this sensing array strategy, we have successfully identified six human cell lines, including one normal type and five tumor types.					
34499479	6	29	theme	cell	1258:1261	arg1	lines					1263:1267	six human cell lines	1248:1267	six human cell lines	1248:1267	Using this sensing array strategy, we have successfully identified six human cell lines, including one normal type and five tumor types.					
34499479	7	30	theme	different	1361:1369	arg1	lines					1376:1380	different cell lines	1361:1380	different cell lines with HeLa cells	1361:1396	Moreover, cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis has also been successfully achieved.					
34499479	2	31	theme	urgent	393:398	arg1	need					400:403	an urgent need	390:403	an urgent need for a more effective strategy to address the problem	390:456	This situation is becoming worse over time due to the rapid growth of the biomedical field, and thus there is an urgent need for a more effective strategy to address the problem.					
34499479	3	32	theme	due	723:725	arg1	properties					712:721	distinct fluorescent properties	691:721	distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules	691:787	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	4	33	theme	endocytosis	918:928	arg1	degrees					930:936	their endocytosis degrees	912:936	their endocytosis degrees	912:936	The different affinity between each nanoparticle and various cell lines results in clearly distinguishable differences in their endocytosis degrees, thus resulting in distinct characteristic fluorescence intensities.					
34499479	6	34	theme	human	1252:1256	arg1	type					1291:1294	one normal type	1280:1294	one normal type	1280:1294	Using this sensing array strategy, we have successfully identified six human cell lines, including one normal type and five tumor types.					
34499479	6	34	theme	human	1252:1256	arg1	lines					1263:1267	six human cell lines	1248:1267	six human cell lines	1248:1267	Using this sensing array strategy, we have successfully identified six human cell lines, including one normal type and five tumor types.					
34499479	3	35	theme	distinct	691:698	arg1	properties					712:721	distinct fluorescent properties	691:721	distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules	691:787	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	6	36	theme	array	1200:1204	arg1	strategy					1206:1213	this sensing array strategy	1187:1213	this sensing array strategy	1187:1213	Using this sensing array strategy, we have successfully identified six human cell lines, including one normal type and five tumor types.					
34499479	1	37	theme	biomedicine	181:191	arg1	field					172:176	the field	168:176	the field of biomedicine	168:191	Cell lines are applied on a large scale in the field of biomedicine, but they are susceptible to issues such as misidentification and cross-contamination.					
34499479	0	38	theme	Fluorescent	28:38	arg1	Nanoparticles					47:59	Fluorescent Glycan Nanoparticles	28:59	Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation	28:122	Cell Coding Arrays Based on Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation.					
34499479	3	39	theme	fluorescent	700:710	arg1	properties					712:721	distinct fluorescent properties	691:721	distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules	691:787	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	4	40	theme	different	794:802	arg1	affinity					804:811	The different affinity	790:811	The different affinity between each nanoparticle and various cell lines	790:860	The different affinity between each nanoparticle and various cell lines results in clearly distinguishable differences in their endocytosis degrees, thus resulting in distinct characteristic fluorescence intensities.					
34499479	2	41	theme	effective	416:424	arg1	strategy					426:433	a more effective strategy	409:433	a more effective strategy to address the problem	409:456	This situation is becoming worse over time due to the rapid growth of the biomedical field, and thus there is an urgent need for a more effective strategy to address the problem.					
34499479	3	42	theme	nanoparticles	564:576	arg1	types					529:533	two types	525:533	two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules	525:787	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	5	43	theme	statistical	1157:1167	arg1	processing					1169:1178	statistical processing	1157:1178	statistical processing	1157:1178	Through flow cytometry measurements, the specific signals of each cell sample can be recorded and turned into a map divided into different regions by statistical processing.					
34499479	4	44	theme	distinguishable	881:895	arg1	differences					897:907	clearly distinguishable differences	873:907	clearly distinguishable differences in their endocytosis degrees	873:936	The different affinity between each nanoparticle and various cell lines results in clearly distinguishable differences in their endocytosis degrees, thus resulting in distinct characteristic fluorescence intensities.					
34499479	5	45	theme	specific	1048:1055	arg1	signals					1057:1063	the specific signals	1044:1063	the specific signals of each cell sample	1044:1083	Through flow cytometry measurements, the specific signals of each cell sample can be recorded and turned into a map divided into different regions by statistical processing.					
34499479	8	46	theme	testing	1556:1562	arg1	process					1504:1510	the whole process	1494:1510	the whole process of nanoparticle fabrication and fluorescent testing	1494:1562	Notably, the whole process of nanoparticle fabrication and fluorescent testing is facile and the results are highly reliable.					
34499479	8	46	theme	testing	1556:1562	arg1	facile					1567:1572	facile	1567:1572	facile	1567:1572	Notably, the whole process of nanoparticle fabrication and fluorescent testing is facile and the results are highly reliable.					
34499479	0	47	theme	Glycan	40:45	arg1	Nanoparticles					47:59	Fluorescent Glycan Nanoparticles	28:59	Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation	28:122	Cell Coding Arrays Based on Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation.					
34499479	3	48	theme	graft-copolymerization-induced	609:638	arg1	method					661:666	the graft-copolymerization-induced self-assembly (GISA) method	605:666	the graft-copolymerization-induced self-assembly (GISA) method	605:666	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	4	49	from	differences	897:907	arg1	degrees					930:936	their endocytosis degrees	912:936	their endocytosis degrees	912:936	The different affinity between each nanoparticle and various cell lines results in clearly distinguishable differences in their endocytosis degrees, thus resulting in distinct characteristic fluorescence intensities.					
34499479	7	50	theme	contamination	1333:1345	arg1	evaluation					1347:1356	cell contamination evaluation	1328:1356	cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis	1328:1446	Moreover, cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis has also been successfully achieved.					
34499479	1	51	theme	Cell	125:128	arg1	lines					130:134	Cell lines	125:134	Cell lines	125:134	Cell lines are applied on a large scale in the field of biomedicine, but they are susceptible to issues such as misidentification and cross-contamination.					
34499479	6	52	theme	tumor	1305:1309	arg1	types					1311:1315	five tumor types	1300:1315	five tumor types	1300:1315	Using this sensing array strategy, we have successfully identified six human cell lines, including one normal type and five tumor types.					
34499479	6	52	theme	tumor	1305:1309	arg1	type					1291:1294	one normal type	1280:1294	one normal type	1280:1294	Using this sensing array strategy, we have successfully identified six human cell lines, including one normal type and five tumor types.					
34499479	3	53	theme	self-assembly	640:652	arg1	method					661:666	the graft-copolymerization-induced self-assembly (GISA) method	605:666	the graft-copolymerization-induced self-assembly (GISA) method	605:666	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	3	54	theme	stable	550:555	arg1	nanoparticles					564:576	uniform and stable glycan nanoparticles	538:576	uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules	538:787	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	7	55	theme	semiquantitative	1422:1437	arg1	analysis					1439:1446	a semiquantitative analysis	1420:1446	a semiquantitative analysis	1420:1446	Moreover, cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis has also been successfully achieved.					
34499479	5	56	theme	cell	1073:1076	arg1	sample					1078:1083	each cell sample	1068:1083	each cell sample	1068:1083	Through flow cytometry measurements, the specific signals of each cell sample can be recorded and turned into a map divided into different regions by statistical processing.					
34499479	3	57	theme	glycan	557:562	arg1	nanoparticles					564:576	uniform and stable glycan nanoparticles	538:576	uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules	538:787	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	7	58	theme	HeLa	1387:1390	arg1	cells					1392:1396	HeLa cells	1387:1396	HeLa cells	1387:1396	Moreover, cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis has also been successfully achieved.					
34499479	7	59	from	contaminant	1405:1415	arg1	analysis					1439:1446	a semiquantitative analysis	1420:1446	a semiquantitative analysis	1420:1446	Moreover, cell contamination evaluation of different cell lines with HeLa cells as the contaminant in a semiquantitative analysis has also been successfully achieved.					
34499479	0	60	theme	Cell	65:68	arg1	Identification					75:88	Cell Line Identification	65:88	Cell Line Identification	65:88	Cell Coding Arrays Based on Fluorescent Glycan Nanoparticles for Cell Line Identification and Cell Contamination Evaluation.					
34499479	5	61	theme	sample	1078:1083	arg1	signals					1057:1063	the specific signals	1044:1063	the specific signals of each cell sample	1044:1083	Through flow cytometry measurements, the specific signals of each cell sample can be recorded and turned into a map divided into different regions by statistical processing.					
34499479	3	62	theme	uniform	538:544	arg1	nanoparticles					564:576	uniform and stable glycan nanoparticles	538:576	uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules	538:787	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	5	63	theme	cytometry	1020:1028	arg1	measurements					1030:1041	flow cytometry measurements	1015:1041	flow cytometry measurements	1015:1041	Through flow cytometry measurements, the specific signals of each cell sample can be recorded and turned into a map divided into different regions by statistical processing.					
34499479	3	64	theme	fluorescent	758:768	arg1	molecules					779:787	fluorescent labeling molecules	758:787	fluorescent labeling molecules	758:787	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
34499479	4	65	theme	characteristic	966:979	arg1	intensities					994:1004	distinct characteristic fluorescence intensities	957:1004	distinct characteristic fluorescence intensities	957:1004	The different affinity between each nanoparticle and various cell lines results in clearly distinguishable differences in their endocytosis degrees, thus resulting in distinct characteristic fluorescence intensities.					
34499479	1	66	from	scale	159:163	arg1	field					172:176	the field	168:176	the field of biomedicine	168:191	Cell lines are applied on a large scale in the field of biomedicine, but they are susceptible to issues such as misidentification and cross-contamination.					
34499479	8	67	theme	nanoparticle	1515:1526	arg1	fabrication					1528:1538	nanoparticle fabrication	1515:1538	nanoparticle fabrication	1515:1538	Notably, the whole process of nanoparticle fabrication and fluorescent testing is facile and the results are highly reliable.					
34499479	3	68	theme	labeling	770:777	arg1	molecules					779:787	fluorescent labeling molecules	758:787	fluorescent labeling molecules	758:787	As described herein, a cell coding method is established based on two types of uniform and stable glycan nanoparticles that are synthesized using the graft-copolymerization-induced self-assembly (GISA) method, which further exhibit distinct fluorescent properties due to elaborate modification with fluorescent labeling molecules.					
33084194	9	0	theme	malondialdehyde	1499:1513	arg1	level					1490:1494	the level	1486:1494	the level of malondialdehyde (MDA)	1486:1519	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	5	1	theme	Electron	774:781	arg1	SEM					795:797	SEM	795:797	SEM	795:797	Crystallinity of LAP was inferred from its X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM) analysis.					
33084194	5	1	theme	Electron	774:781	arg1	Microscopy					783:792	Scanning Electron Microscopy	765:792	Scanning Electron Microscopy (SEM) analysis	765:807	Crystallinity of LAP was inferred from its X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM) analysis.					
33084194	0	2	theme	Antioxidant	67:77	arg1	Activities					79:88	in Vitro Antioxidant Activities	58:88	in Vitro Antioxidant Activities	58:88	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	3	3	theme	arabinose	462:470	arg1	presence					440:447	the presence	436:447	the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6	436:555	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	0	4	theme	Protective	94:103	arg1	Effect					105:110	Protective Effect	94:110	Protective Effect	94:110	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	8	5	theme	hemolysis	1288:1296	arg1	Inhibition					1262:1271	Inhibition	1262:1271	Inhibition of erythrocyte hemolysis and lipid peroxidation	1262:1319	Inhibition of erythrocyte hemolysis and lipid peroxidation was also assessed.					
33084194	4	6	theme	GC/MS	664:668	arg1	data					685:688	its GC/MS, FT-IR and NMR data	660:688	its GC/MS, FT-IR and NMR data	660:688	The extracted polysaccharide (PS) was considered as heterogeneous and highly branched by interpreting its GC/MS, FT-IR and NMR data.					
33084194	1	7	theme	Lycium	248:253	arg1	arabicum					255:262	Lycium arabicum	248:262	Lycium arabicum	248:262	In this research work, a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum was investigated.					
33084194	9	8	theme	2,2-azobis	1429:1438	arg1	AAPH					1423:1426	AAPH	1423:1426	AAPH (2,2-azobis(2-amidinopropane) dihydrochloride)	1423:1473	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	9	8	theme	2,2-azobis	1429:1438	arg1	dihydrochloride					1458:1472	2,2-azobis(2-amidinopropane) dihydrochloride	1429:1472	2,2-azobis(2-amidinopropane) dihydrochloride	1429:1472	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	3	9	theme	mannose	496:502	arg1	presence					440:447	the presence	436:447	the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6	436:555	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	1	10	theme	arabicum	255:262	arg1	fruits					238:243	the fruits	234:243	the fruits of Lycium arabicum	234:262	In this research work, a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum was investigated.					
33084194	0	11	from	Features	48:55	arg1	Erythrocytes					146:157	Human Erythrocytes	140:157	Human Erythrocytes	140:157	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	4	12	theme	FT-IR	671:675	arg1	data					685:688	its GC/MS, FT-IR and NMR data	660:688	its GC/MS, FT-IR and NMR data	660:688	The extracted polysaccharide (PS) was considered as heterogeneous and highly branched by interpreting its GC/MS, FT-IR and NMR data.					
33084194	1	13	theme	research	168:175	arg1	work					177:180	this research work	163:180	this research work	163:180	In this research work, a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum was investigated.					
33084194	7	14	theme	low	1024:1026	arg1	concentrations					1028:1041	low concentrations	1024:1041	low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging	1024:1114	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	5	15	theme	Microscopy	783:792	arg1	analysis					800:807	Scanning Electron Microscopy (SEM) analysis	765:807	Scanning Electron Microscopy (SEM) analysis	765:807	Crystallinity of LAP was inferred from its X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM) analysis.					
33084194	3	16	theme	glucose	484:490	arg1	presence					440:447	the presence	436:447	the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6	436:555	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	2	17	theme	uronic	352:357	arg1	acids					359:363	uronic acids	352:363	uronic acids (3.56±0.34 %)	352:377	LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %).					
33084194	2	17	theme	uronic	352:357	arg1	%					376:376	3.56±0.34 %	366:376	3.56±0.34 %	366:376	LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %).					
33084194	7	18	theme	antioxidant	1000:1010	arg1	activity					1012:1019	a strong antioxidant activity	991:1019	a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging	991:1114	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	6	19	theme	interesting	827:837	arg1	stability					839:847	an interesting stability	824:847	an interesting stability	824:847	LAP exhibited an interesting stability at high temperatures (∼254 °C) and in a wide range of pH (3-9) deduced, respectively, from its DSC and zeta potential analysis.					
33084194	3	20	from	presence	440:447	arg1	ratio					515:519	a molar ratio	507:519	a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6	507:555	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	21	theme	molar	509:513	arg1	ratio					515:519	a molar ratio	507:519	a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6	507:555	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	7	22	theme	ferric	1117:1122	arg1	FRAP					1149:1152	FRAP	1149:1152	FRAP	1149:1152	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	7	22	theme	ferric	1117:1122	arg1	power					1142:1146	ferric reducing activity power	1117:1146	ferric reducing activity power (FRAP)	1117:1153	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	9	23	theme	reduced	1548:1554	arg1	GSH					1569:1571	GSH	1569:1571	GSH	1569:1571	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	9	23	theme	reduced	1548:1554	arg1	glutathione					1556:1566	the reduced glutathione	1544:1566	the reduced glutathione (GSH) level	1544:1578	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	6	24	theme	high	852:855	arg1	∼254 °C					871:877	∼254 °C	871:877	∼254 °C	871:877	LAP exhibited an interesting stability at high temperatures (∼254 °C) and in a wide range of pH (3-9) deduced, respectively, from its DSC and zeta potential analysis.					
33084194	6	24	theme	high	852:855	arg1	temperatures					857:868	high temperatures	852:868	high temperatures (∼254 °C)	852:878	LAP exhibited an interesting stability at high temperatures (∼254 °C) and in a wide range of pH (3-9) deduced, respectively, from its DSC and zeta potential analysis.					
33084194	4	25	theme	extracted	562:570	arg1	PS					588:589	PS	588:589	PS	588:589	The extracted polysaccharide (PS) was considered as heterogeneous and highly branched by interpreting its GC/MS, FT-IR and NMR data.					
33084194	4	25	theme	extracted	562:570	arg1	polysaccharide					572:585	The extracted polysaccharide	558:585	The extracted polysaccharide (PS)	558:590	The extracted polysaccharide (PS) was considered as heterogeneous and highly branched by interpreting its GC/MS, FT-IR and NMR data.					
33084194	5	26	theme	LAP	708:710	arg1	Crystallinity					691:703	Crystallinity	691:703	Crystallinity of LAP	691:710	Crystallinity of LAP was inferred from its X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM) analysis.					
33084194	7	27	theme	hydroxyl	1223:1230	arg1	abilities					1251:1259	hydroxyl radical-scavenging abilities	1223:1259	hydroxyl radical-scavenging abilities	1223:1259	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	9	28	theme	2-amidinopropane	1440:1455	arg1	AAPH					1423:1426	AAPH	1423:1426	AAPH (2,2-azobis(2-amidinopropane) dihydrochloride)	1423:1473	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	9	28	theme	2-amidinopropane	1440:1455	arg1	dihydrochloride					1458:1472	2,2-azobis(2-amidinopropane) dihydrochloride	1429:1472	2,2-azobis(2-amidinopropane) dihydrochloride	1429:1472	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	7	29	theme	radical-scavenging	1232:1249	arg1	abilities					1251:1259	hydroxyl radical-scavenging abilities	1223:1259	hydroxyl radical-scavenging abilities	1223:1259	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	1	30	theme	water-soluble	185:197	arg1	polysaccharide					199:212	a water-soluble polysaccharide	183:212	a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum	183:262	In this research work, a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum was investigated.					
33084194	1	30	theme	water-soluble	185:197	arg1	LAP					215:217	LAP	215:217	LAP	215:217	In this research work, a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum was investigated.					
33084194	7	31	theme	strong	993:998	arg1	activity					1012:1019	a strong antioxidant activity	991:1019	a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging	991:1114	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	0	32	theme	Oxidative	120:128	arg1	Damage					130:135	Oxidative Damage	120:135	Oxidative Damage	120:135	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	0	33	from	Activities	79:88	arg1	Erythrocytes					146:157	Human Erythrocytes	140:157	Human Erythrocytes	140:157	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	0	34	dep	in	58:59	arg1	Vitro					61:65	Vitro	61:65	Vitro	61:65	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	3	35	dep	ratio	515:519	arg1	 1.5 					529:533	 1.5 	529:533	 1.5 	529:533	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	35	dep	ratio	515:519	arg1	 5.6					552:555	 5.6	552:555	 5.6	552:555	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	35	dep	ratio	515:519	arg1	 8.7 					539:543	 8.7 	539:543	 8.7 	539:543	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	35	dep	ratio	515:519	arg1	 16.4 					545:550	 16.4 	545:550	 16.4 	545:550	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	35	dep	ratio	515:519	arg1	 1 					535:537	 1 	535:537	 1 	535:537	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	6	36	theme	pH	903:904	arg1	range					894:898	a wide range	887:898	a wide range of pH (3-9)	887:910	LAP exhibited an interesting stability at high temperatures (∼254 °C) and in a wide range of pH (3-9) deduced, respectively, from its DSC and zeta potential analysis.					
33084194	0	37	theme	Structural	37:46	arg1	Features					48:55	Structural Features	37:55	Structural Features	37:55	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	6	38	theme	zeta	952:955	arg1	analysis					967:974	zeta potential analysis	952:974	zeta potential analysis	952:974	LAP exhibited an interesting stability at high temperatures (∼254 °C) and in a wide range of pH (3-9) deduced, respectively, from its DSC and zeta potential analysis.					
33084194	0	39	theme	Human	140:144	arg1	Erythrocytes					146:157	Human Erythrocytes	140:157	Human Erythrocytes	140:157	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	1	40	attach	isolated	220:227	arg2	LAP					215:217	LAP	215:217	LAP	215:217	In this research work, a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum was investigated.					
33084194	1	40	attach	isolated	220:227	arg2	polysaccharide					199:212	a water-soluble polysaccharide	183:212	a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum	183:262	In this research work, a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum was investigated.					
33084194	1	40	attach	isolated	220:227	arg1	fruits					238:243	the fruits	234:243	the fruits of Lycium arabicum	234:262	In this research work, a water-soluble polysaccharide (LAP) isolated from the fruits of Lycium arabicum was investigated.					
33084194	7	41	theme	free	1156:1159	arg1	ability					1180:1186	free radical scavenging ability	1156:1186	free radical scavenging ability	1156:1186	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	9	42	theme	damaged	1392:1398	arg1	erythrocytes					1400:1411	the damaged erythrocytes	1388:1411	the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride)	1388:1473	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	7	43	theme	radical	1161:1167	arg1	ability					1180:1186	free radical scavenging ability	1156:1186	free radical scavenging ability	1156:1186	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	2	44	contain	contains	286:293	arg2	%					376:376	3.56±0.34 %	366:376	3.56±0.34 %	366:376	LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %).					
33084194	2	44	contain	contains	286:293	arg2	%					321:321	82.45±1.23 %	310:321	82.45±1.23 %	310:321	LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %).					
33084194	2	44	contain	contains	286:293	arg2	carbohydrates					295:307	carbohydrates	295:307	carbohydrates (82.45±1.23 %)	295:322	LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %).					
33084194	2	44	contain	contains	286:293	arg2	protein					325:331	protein	325:331	protein (1.56±0.21 %)	325:345	LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %).					
33084194	2	44	contain	contains	286:293	arg1	LAP					282:284	LAP	282:284	LAP	282:284	LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %).					
33084194	2	44	contain	contains	286:293	arg2	acids					359:363	uronic acids	352:363	uronic acids (3.56±0.34 %)	352:377	LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %).					
33084194	2	44	contain	contains	286:293	arg2	%					344:344	1.56±0.21 %	334:344	1.56±0.21 %	334:344	LAP contains carbohydrates (82.45±1.23 %), protein (1.56±0.21 %), and uronic acids (3.56±0.34 %).					
33084194	9	45	theme	glutathione	1556:1566	arg1	level					1574:1578	the reduced glutathione (GSH) level	1544:1578	the reduced glutathione (GSH) level	1544:1578	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	7	46	from	concentrations	1028:1041	arg1	power					1142:1146	ferric reducing activity power	1117:1146	ferric reducing activity power (FRAP)	1117:1153	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	7	46	from	concentrations	1028:1041	arg1	abilities					1251:1259	hydroxyl radical-scavenging abilities	1223:1259	hydroxyl radical-scavenging abilities	1223:1259	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	7	46	from	concentrations	1028:1041	arg1	FRAP					1149:1152	FRAP	1149:1152	FRAP	1149:1152	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	7	46	from	concentrations	1028:1041	arg1	ability					1180:1186	free radical scavenging ability	1156:1186	free radical scavenging ability	1156:1186	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	7	46	from	concentrations	1028:1041	arg1	radical-scavenging					1200:1217	superoxide radical-scavenging	1189:1217	superoxide radical-scavenging	1189:1217	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	7	46	from	concentrations	1028:1041	arg1	activity					1012:1019	a strong antioxidant activity	991:1019	a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging	991:1114	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	3	47	theme	monosaccharide	400:413	arg1	composition					415:425	the monosaccharide composition	396:425	the monosaccharide composition	396:425	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	6	48	dep	exhibited	814:822	arg1	deduced					912:918	deduced	912:918	deduced	912:918	LAP exhibited an interesting stability at high temperatures (∼254 °C) and in a wide range of pH (3-9) deduced, respectively, from its DSC and zeta potential analysis.					
33084194	8	49	theme	erythrocyte	1276:1286	arg1	hemolysis					1288:1296	erythrocyte hemolysis	1276:1296	erythrocyte hemolysis	1276:1296	Inhibition of erythrocyte hemolysis and lipid peroxidation was also assessed.					
33084194	6	50	theme	potential	957:965	arg1	analysis					967:974	zeta potential analysis	952:974	zeta potential analysis	952:974	LAP exhibited an interesting stability at high temperatures (∼254 °C) and in a wide range of pH (3-9) deduced, respectively, from its DSC and zeta potential analysis.					
33084194	9	51	theme	erythrocytes	1400:1411	arg1	protection					1374:1383	the protection	1370:1383	the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride)	1370:1473	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	3	52	theme	4.7 	524:527	arg1	ratio					515:519	a molar ratio	507:519	a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6	507:555	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	7	53	theme	reducing	1124:1131	arg1	FRAP					1149:1152	FRAP	1149:1152	FRAP	1149:1152	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	7	53	theme	reducing	1124:1131	arg1	power					1142:1146	ferric reducing activity power	1117:1146	ferric reducing activity power (FRAP)	1117:1153	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	3	54	theme	rhamnose	452:459	arg1	presence					440:447	the presence	436:447	the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6	436:555	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	8	55	theme	lipid	1302:1306	arg1	peroxidation					1308:1319	lipid peroxidation	1302:1319	lipid peroxidation	1302:1319	Inhibition of erythrocyte hemolysis and lipid peroxidation was also assessed.					
33084194	0	56	from	Effect	105:110	arg1	Erythrocytes					146:157	Human Erythrocytes	140:157	Human Erythrocytes	140:157	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	4	57	theme	NMR	681:683	arg1	data					685:688	its GC/MS, FT-IR and NMR data	660:688	its GC/MS, FT-IR and NMR data	660:688	The extracted polysaccharide (PS) was considered as heterogeneous and highly branched by interpreting its GC/MS, FT-IR and NMR data.					
33084194	6	58	theme	wide	889:892	arg1	range					894:898	a wide range	887:898	a wide range of pH (3-9)	887:910	LAP exhibited an interesting stability at high temperatures (∼254 °C) and in a wide range of pH (3-9) deduced, respectively, from its DSC and zeta potential analysis.					
33084194	9	59	dep	allowed	1362:1368	arg1	increase					1535:1542	increase	1535:1542	to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level	1476:1578	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	9	59	dep	allowed	1362:1368	arg1	reduce					1479:1484	reduce	1479:1484	to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level	1476:1578	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	7	60	theme	activity	1133:1140	arg1	FRAP					1149:1152	FRAP	1149:1152	FRAP	1149:1152	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	7	60	theme	activity	1133:1140	arg1	power					1142:1146	ferric reducing activity power	1117:1146	ferric reducing activity power (FRAP)	1117:1153	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	7	61	theme	superoxide	1189:1198	arg1	radical-scavenging					1200:1217	superoxide radical-scavenging	1189:1217	superoxide radical-scavenging	1189:1217	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	5	62	theme	Scanning	765:772	arg1	SEM					795:797	SEM	795:797	SEM	795:797	Crystallinity of LAP was inferred from its X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM) analysis.					
33084194	5	62	theme	Scanning	765:772	arg1	Microscopy					783:792	Scanning Electron Microscopy	765:792	Scanning Electron Microscopy (SEM) analysis	765:807	Crystallinity of LAP was inferred from its X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM) analysis.					
33084194	9	63	theme	LAP	1348:1350	arg1	treatment					1352:1360	LAP treatment	1348:1360	LAP treatment	1348:1360	In 5 h, LAP treatment allowed the protection of the damaged erythrocytes caused by AAPH (2,2-azobis(2-amidinopropane) dihydrochloride), to reduce the level of malondialdehyde (MDA) as well as to increase the reduced glutathione (GSH) level.					
33084194	5	64	theme	X-ray	734:738	arg1	XRD					756:758	XRD	756:758	XRD	756:758	Crystallinity of LAP was inferred from its X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM) analysis.					
33084194	5	64	theme	X-ray	734:738	arg1	diffractometry					740:753	its X-ray diffractometry	730:753	its X-ray diffractometry (XRD)	730:759	Crystallinity of LAP was inferred from its X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM) analysis.					
33084194	8	65	theme	peroxidation	1308:1319	arg1	Inhibition					1262:1271	Inhibition	1262:1271	Inhibition of erythrocyte hemolysis and lipid peroxidation	1262:1319	Inhibition of erythrocyte hemolysis and lipid peroxidation was also assessed.					
33084194	3	66	theme	composition	415:425	arg1	analysis					384:391	The analysis	380:391	The analysis of the monosaccharide composition	380:425	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	7	67	theme	-radical	1096:1103	arg1	scavenging					1105:1114	the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging	1056:1114	the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging	1056:1114	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	7	68	theme	scavenging	1169:1178	arg1	ability					1180:1186	free radical scavenging ability	1156:1186	free radical scavenging ability	1156:1186	LAP displayed a strong antioxidant activity at low concentrations evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, ferric reducing activity power (FRAP), free radical scavenging ability, superoxide radical-scavenging and hydroxyl radical-scavenging abilities.					
33084194	0	69	theme	in	58:59	arg1	Activities					79:88	in Vitro Antioxidant Activities	58:88	in Vitro Antioxidant Activities	58:88	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	0	70	from	arabicum	27:34	arg1	Polysaccharide					0:13	Polysaccharide	0:13	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.	0:158	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	0	71	dep	Polysaccharide	0:13	arg1	Effect					105:110	Protective Effect	94:110	Protective Effect	94:110	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	0	71	dep	Polysaccharide	0:13	arg1	Activities					79:88	in Vitro Antioxidant Activities	58:88	in Vitro Antioxidant Activities	58:88	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	0	71	dep	Polysaccharide	0:13	arg1	Features					48:55	Structural Features	37:55	Structural Features	37:55	Polysaccharide from Lycium arabicum: Structural Features, in Vitro Antioxidant Activities and Protective Effect against Oxidative Damage in Human Erythrocytes.					
33084194	3	72	attach	presence	440:447	arg2	rhamnose					452:459	rhamnose	452:459	rhamnose	452:459	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	72	attach	presence	440:447	arg2	glucose					484:490	glucose	484:490	glucose	484:490	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	72	attach	presence	440:447	arg2	arabinose					462:470	arabinose	462:470	arabinose	462:470	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	72	attach	presence	440:447	arg2	mannose					496:502	mannose	496:502	mannose	496:502	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	72	attach	presence	440:447	arg1	ratio					515:519	a molar ratio	507:519	a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6	507:555	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	72	attach	presence	440:447	arg2	galactose					473:481	galactose	473:481	galactose	473:481	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
33084194	3	73	theme	galactose	473:481	arg1	presence					440:447	the presence	436:447	the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6	436:555	The analysis of the monosaccharide composition revealed the presence of rhamnose, arabinose, galactose, glucose and mannose in a molar ratio of 4.7 : 1.5 : 1 : 8.7 : 16.4 : 5.6.					
32507190	0	0	theme	antimicrobial	80:92	arg1	properties					94:103	optical and antimicrobial properties	68:103	optical and antimicrobial properties	68:103	Porous composites based on cellulose acetate and alfa-hematite with optical and antimicrobial properties.					
32507190	4	1	theme	cellulose	544:552	arg1	acetate					554:560	cellulose acetate	544:560	cellulose acetate (CA) polymer	544:573	In this work, hematite NPs were blended with cellulose acetate (CA) polymer and thin nanocomposite films were produced by the non-solvent induced phase separation technique.					
32507190	4	1	theme	cellulose	544:552	arg1	CA					563:564	CA	563:564	CA	563:564	In this work, hematite NPs were blended with cellulose acetate (CA) polymer and thin nanocomposite films were produced by the non-solvent induced phase separation technique.					
32507190	5	2	theme	superior	756:763	arg1	character					765:773	its superior character	752:773	its superior character	752:773	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	3	3	theme	nanosized	480:488	arg1	properties					466:475	their inherent properties	451:475	their inherent properties	451:475	On the other hand, NPs can bring into the composites their inherent properties as nanosized objects.					
32507190	3	3	theme	nanosized	480:488	arg1	objects					490:496	nanosized objects	480:496	nanosized objects	480:496	On the other hand, NPs can bring into the composites their inherent properties as nanosized objects.					
32507190	5	4	theme	further	931:937	arg1	validation					939:948	its further validation	927:948	its further validation	927:948	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	4	5	theme	acetate	554:560	arg1	polymer					567:573	cellulose acetate (CA) polymer	544:573	cellulose acetate (CA) polymer	544:573	In this work, hematite NPs were blended with cellulose acetate (CA) polymer and thin nanocomposite films were produced by the non-solvent induced phase separation technique.					
32507190	4	6	theme	separation	651:660	arg1	technique					662:670	the non-solvent induced phase separation technique	621:670	the non-solvent induced phase separation technique	621:670	In this work, hematite NPs were blended with cellulose acetate (CA) polymer and thin nanocomposite films were produced by the non-solvent induced phase separation technique.					
32507190	5	7	theme	electronic/optical	817:834	arg1	terms					778:782	terms	778:782	terms of structure, thermal stability, electronic/optical and antibacterial properties	778:863	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	5	8	theme	adsorptive	953:962	arg1	applications					983:994	adsorptive and photocatalytic applications	953:994	adsorptive and photocatalytic applications	953:994	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	4	9	theme	non-solvent	625:635	arg1	technique					662:670	the non-solvent induced phase separation technique	621:670	the non-solvent induced phase separation technique	621:670	In this work, hematite NPs were blended with cellulose acetate (CA) polymer and thin nanocomposite films were produced by the non-solvent induced phase separation technique.					
32507190	5	10	theme	properties	854:863	arg1	terms					778:782	terms	778:782	terms of structure, thermal stability, electronic/optical and antibacterial properties	778:863	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	5	11	theme	composite	722:730	arg1	materials					732:740	these composite materials	716:740	these composite materials	716:740	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	5	12	theme	structure	787:795	arg1	terms					778:782	terms	778:782	terms of structure, thermal stability, electronic/optical and antibacterial properties	778:863	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	5	13	theme	materials	732:740	arg1	characterization					696:711	A full physicochemical characterization	673:711	A full physicochemical characterization of these composite materials	673:740	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	3	14	theme	other	405:409	arg1	hand					411:414	the other hand	401:414	the other hand	401:414	On the other hand, NPs can bring into the composites their inherent properties as nanosized objects.					
32507190	0	15	theme	Porous	0:5	arg1	composites					7:16	Porous composites	0:16	Porous composites	0:16	Porous composites based on cellulose acetate and alfa-hematite with optical and antimicrobial properties.					
32507190	2	16	theme	magnetic	311:318	arg1	forces					338:343	the attractive magnetic and van der Waals forces	296:343	the attractive magnetic and van der Waals forces that act on NPs and thus reduce their agglomeration	296:395	On one hand, polymers contribute to balance the attractive magnetic and van der Waals forces that act on NPs and thus reduce their agglomeration.					
32507190	1	17	theme	high	188:191	arg1	potential					193:201	their high potential	182:201	their high potential for application in several technological fields	182:249	Polymer nanoparticles (NPs) composites are rapidly gaining attention due to their high potential for application in several technological fields.					
32507190	5	18	theme	thermal	798:804	arg1	stability					806:814	thermal stability	798:814	thermal stability	798:814	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	4	19	theme	hematite	513:520	arg1	NPs					522:524	hematite NPs	513:524	hematite NPs	513:524	In this work, hematite NPs were blended with cellulose acetate (CA) polymer and thin nanocomposite films were produced by the non-solvent induced phase separation technique.					
32507190	5	20	from	polymer	886:892	arg1	own					901:903	own	901:903	own	901:903	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	1	21	theme	Polymer	106:112	arg1	NPs					129:131	NPs	129:131	NPs	129:131	Polymer nanoparticles (NPs) composites are rapidly gaining attention due to their high potential for application in several technological fields.					
32507190	1	21	theme	Polymer	106:112	arg1	nanoparticles					114:126	Polymer nanoparticles	106:126	Polymer nanoparticles (NPs) composites	106:143	Polymer nanoparticles (NPs) composites are rapidly gaining attention due to their high potential for application in several technological fields.					
32507190	4	22	theme	nanocomposite	584:596	arg1	films					598:602	thin nanocomposite films	579:602	thin nanocomposite films	579:602	In this work, hematite NPs were blended with cellulose acetate (CA) polymer and thin nanocomposite films were produced by the non-solvent induced phase separation technique.					
32507190	5	23	theme	stability	806:814	arg1	terms					778:782	terms	778:782	terms of structure, thermal stability, electronic/optical and antibacterial properties	778:863	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	1	24	theme	nanoparticles	114:126	arg1	composites					134:143	Polymer nanoparticles (NPs) composites	106:143	Polymer nanoparticles (NPs) composites	106:143	Polymer nanoparticles (NPs) composites are rapidly gaining attention due to their high potential for application in several technological fields.					
32507190	4	25	theme	thin	579:582	arg1	films					598:602	thin nanocomposite films	579:602	thin nanocomposite films	579:602	In this work, hematite NPs were blended with cellulose acetate (CA) polymer and thin nanocomposite films were produced by the non-solvent induced phase separation technique.					
32507190	5	26	theme	full	675:678	arg1	characterization					696:711	A full physicochemical characterization	673:711	A full physicochemical characterization of these composite materials	673:740	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	0	27	theme	cellulose	27:35	arg1	acetate					37:43	cellulose acetate	27:43	cellulose acetate	27:43	Porous composites based on cellulose acetate and alfa-hematite with optical and antimicrobial properties.					
32507190	5	28	theme	physicochemical	680:694	arg1	characterization					696:711	A full physicochemical characterization	673:711	A full physicochemical characterization of these composite materials	673:740	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	1	29	from	application	207:217	arg1	fields					244:249	several technological fields	222:249	several technological fields	222:249	Polymer nanoparticles (NPs) composites are rapidly gaining attention due to their high potential for application in several technological fields.					
32507190	5	30	theme	antibacterial	840:852	arg1	properties					854:863	antibacterial properties	840:863	antibacterial properties	840:863	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	3	31	theme	inherent	457:464	arg1	properties					466:475	their inherent properties	451:475	their inherent properties	451:475	On the other hand, NPs can bring into the composites their inherent properties as nanosized objects.					
32507190	3	31	theme	inherent	457:464	arg1	objects					490:496	nanosized objects	480:496	nanosized objects	480:496	On the other hand, NPs can bring into the composites their inherent properties as nanosized objects.					
32507190	2	32	theme	Waals	332:336	arg1	forces					338:343	the attractive magnetic and van der Waals forces	296:343	the attractive magnetic and van der Waals forces that act on NPs and thus reduce their agglomeration	296:395	On one hand, polymers contribute to balance the attractive magnetic and van der Waals forces that act on NPs and thus reduce their agglomeration.					
32507190	5	33	theme	photocatalytic	968:981	arg1	applications					983:994	adsorptive and photocatalytic applications	953:994	adsorptive and photocatalytic applications	953:994	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	2	34	theme	der	328:330	arg1	forces					338:343	the attractive magnetic and van der Waals forces	296:343	the attractive magnetic and van der Waals forces that act on NPs and thus reduce their agglomeration	296:395	On one hand, polymers contribute to balance the attractive magnetic and van der Waals forces that act on NPs and thus reduce their agglomeration.					
32507190	2	35	theme	attractive	300:309	arg1	forces					338:343	the attractive magnetic and van der Waals forces	296:343	the attractive magnetic and van der Waals forces that act on NPs and thus reduce their agglomeration	296:395	On one hand, polymers contribute to balance the attractive magnetic and van der Waals forces that act on NPs and thus reduce their agglomeration.					
32507190	1	36	theme	several	222:228	arg1	fields					244:249	several technological fields	222:249	several technological fields	222:249	Polymer nanoparticles (NPs) composites are rapidly gaining attention due to their high potential for application in several technological fields.					
32507190	0	37	with	alfa-hematite	49:61	arg1	properties					94:103	optical and antimicrobial properties	68:103	optical and antimicrobial properties	68:103	Porous composites based on cellulose acetate and alfa-hematite with optical and antimicrobial properties.					
32507190	2	38	theme	van	324:326	arg1	forces					338:343	the attractive magnetic and van der Waals forces	296:343	the attractive magnetic and van der Waals forces that act on NPs and thus reduce their agglomeration	296:395	On one hand, polymers contribute to balance the attractive magnetic and van der Waals forces that act on NPs and thus reduce their agglomeration.					
32507190	5	39	from	door	919:922	arg1	applications					983:994	adsorptive and photocatalytic applications	953:994	adsorptive and photocatalytic applications	953:994	A full physicochemical characterization of these composite materials confirmed its superior character in terms of structure, thermal stability, electronic/optical and antibacterial properties when compared to the polymer on its own and opens the door to its further validation in adsorptive and photocatalytic applications.					
32507190	1	40	theme	technological	230:242	arg1	fields					244:249	several technological fields	222:249	several technological fields	222:249	Polymer nanoparticles (NPs) composites are rapidly gaining attention due to their high potential for application in several technological fields.					
32507190	0	41	theme	optical	68:74	arg1	properties					94:103	optical and antimicrobial properties	68:103	optical and antimicrobial properties	68:103	Porous composites based on cellulose acetate and alfa-hematite with optical and antimicrobial properties.					
32507190	4	42	theme	phase	645:649	arg1	technique					662:670	the non-solvent induced phase separation technique	621:670	the non-solvent induced phase separation technique	621:670	In this work, hematite NPs were blended with cellulose acetate (CA) polymer and thin nanocomposite films were produced by the non-solvent induced phase separation technique.					
32507190	0	43	with	acetate	37:43	arg1	properties					94:103	optical and antimicrobial properties	68:103	optical and antimicrobial properties	68:103	Porous composites based on cellulose acetate and alfa-hematite with optical and antimicrobial properties.					
32507190	4	44	theme	induced	637:643	arg1	technique					662:670	the non-solvent induced phase separation technique	621:670	the non-solvent induced phase separation technique	621:670	In this work, hematite NPs were blended with cellulose acetate (CA) polymer and thin nanocomposite films were produced by the non-solvent induced phase separation technique.					
34274819	5	0	theme	monosaccharide	502:515	arg1	composition					517:527	The monosaccharide composition	498:527	The monosaccharide composition	498:527	The monosaccharide composition was analyzed by GC-MS.					
34274819	10	1	theme	immumomodulatory	991:1006	arg1	activities					1008:1017	The immumomodulatory activities	987:1017	The immumomodulatory activities of Chachi polysaccharides	987:1043	The immumomodulatory activities of Chachi polysaccharides were improved as the storage time extended.					
34274819	10	1	theme	immumomodulatory	991:1006	arg1	time					1074:1077	the storage time	1062:1077	the storage time extended	1062:1086	The immumomodulatory activities of Chachi polysaccharides were improved as the storage time extended.					
34274819	7	2	theme	α-L-Araf-	701:709	arg1	-β-D-Glcp-					797:806	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→	701:809	-β-D-Glcp-	797:806	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→ were detected.					
34274819	6	3	with	arabinogalacturonan	623:641	arg1	chain					672:676	side chain	667:676	side chain of →4)-β-D-Galp-(1 → 	667:698	NMR data revealed that Chachi polysaccharides were mainly consisted of arabinogalacturonan and another pectin with side chain of →4)-β-D-Galp-(1 → .					
34274819	4	4	theme	As	367:368	arg1	polysaccharides					380:394	As bioactive polysaccharides	367:394	As bioactive polysaccharides	367:394	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	1	5	theme	Chachiennsis	149:160	arg1	peel					122:125	The peel	118:125	The peel of Citrus reticulate 'Chachiennsis' (Chachi)	118:170	The peel of Citrus reticulate 'Chachiennsis' (Chachi) is widely accepted as a functional food.					
34274819	1	5	theme	Chachiennsis	149:160	arg1	food					207:210	a functional food	194:210	a functional food	194:210	The peel of Citrus reticulate 'Chachiennsis' (Chachi) is widely accepted as a functional food.					
34274819	3	6	theme	responsible	319:329	arg1	chemicals					309:317	the chemicals	305:317	the chemicals responsible for this phenomonon	305:349	However, the chemicals responsible for this phenomonon remain unclear.					
34274819	0	7	from	Characterization	0:15	arg1	Chachi					67:72	Chachi	67:72	Chachi	67:72	Characterization of polysaccharide structure in Citrus reticulate 'Chachi' peel during storage and their bioactivity.					
34274819	6	8	with	pectin	655:660	arg1	chain					672:676	side chain	667:676	side chain of →4)-β-D-Galp-(1 → 	667:698	NMR data revealed that Chachi polysaccharides were mainly consisted of arabinogalacturonan and another pectin with side chain of →4)-β-D-Galp-(1 → .					
34274819	11	9	theme	weight	1113:1118	arg1	responsible					1124:1134	responsible	1124:1134	responsible	1124:1134	The change of molecular weight was responsible for the improved immunomodulatory activity.					
34274819	11	9	theme	weight	1113:1118	arg1	change					1093:1098	The change	1089:1098	The change of molecular weight	1089:1118	The change of molecular weight was responsible for the improved immunomodulatory activity.					
34274819	4	10	dep	Chachi	420:425	arg1	prepared					488:495	prepared	488:495	were prepared	483:495	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	4	11	theme	storage	456:462	arg1	times					464:468	various storage times	448:468	various storage times (5-20 years)	448:481	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	4	11	theme	storage	456:462	arg1	years					476:480	5-20 years	471:480	5-20 years	471:480	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	8	12	theme	storage	911:917	arg1	time					919:922	storage time	911:922	storage time	911:922	The molecular weight of Chachi polysaccharides decreased along with the extension of storage time.					
34274819	8	13	theme	polysaccharides	857:871	arg1	weight					840:845	The molecular weight	826:845	The molecular weight of Chachi polysaccharides	826:871	The molecular weight of Chachi polysaccharides decreased along with the extension of storage time.					
34274819	2	14	theme	health	249:254	arg1	benefits					256:263	its health benefits	245:263	its health benefits	245:263	It is generally recognized that its health benefits are dependent on storage time.					
34274819	9	15	theme	basic	938:942	arg1	characteristics					954:968	the basic structure characteristics	934:968	the basic structure characteristics	934:968	However, the basic structure characteristics remained stable.					
34274819	4	16	theme	various	448:454	arg1	times					464:468	various storage times	448:468	various storage times (5-20 years)	448:481	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	4	16	theme	various	448:454	arg1	years					476:480	5-20 years	471:480	5-20 years	471:480	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	4	17	theme	bioactive	370:378	arg1	polysaccharides					380:394	As bioactive polysaccharides	367:394	As bioactive polysaccharides	367:394	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	6	18	theme	-β-D-Galp-	684:693	arg1	chain					672:676	side chain	667:676	side chain of →4)-β-D-Galp-(1 → 	667:698	NMR data revealed that Chachi polysaccharides were mainly consisted of arabinogalacturonan and another pectin with side chain of →4)-β-D-Galp-(1 → .					
34274819	7	19	theme	-α-D-GalpAMe-	755:767	arg1	-β-D-Glcp-					797:806	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→	701:809	-β-D-Glcp-	797:806	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→ were detected.					
34274819	4	20	with	polysaccharides	427:441	arg1	times					464:468	various storage times	448:468	various storage times (5-20 years)	448:481	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	4	20	with	polysaccharides	427:441	arg1	years					476:480	5-20 years	471:480	5-20 years	471:480	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	10	21	theme	polysaccharides	1029:1043	arg1	activities					1008:1017	The immumomodulatory activities	987:1017	The immumomodulatory activities of Chachi polysaccharides	987:1043	The immumomodulatory activities of Chachi polysaccharides were improved as the storage time extended.					
34274819	10	21	theme	polysaccharides	1029:1043	arg1	time					1074:1077	the storage time	1062:1077	the storage time extended	1062:1086	The immumomodulatory activities of Chachi polysaccharides were improved as the storage time extended.					
34274819	2	22	theme	storage	282:288	arg1	time					290:293	storage time	282:293	storage time	282:293	It is generally recognized that its health benefits are dependent on storage time.					
34274819	6	23	theme	Chachi	575:580	arg1	polysaccharides					582:596	Chachi polysaccharides	575:596	Chachi polysaccharides	575:596	NMR data revealed that Chachi polysaccharides were mainly consisted of arabinogalacturonan and another pectin with side chain of →4)-β-D-Galp-(1 → .					
34274819	7	24	theme	-α-D-GalpA-	736:746	arg1	-β-D-Glcp-					797:806	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→	701:809	-β-D-Glcp-	797:806	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→ were detected.					
34274819	11	25	theme	molecular	1103:1111	arg1	weight					1113:1118	molecular weight	1103:1118	molecular weight	1103:1118	The change of molecular weight was responsible for the improved immunomodulatory activity.					
34274819	0	26	theme	structure	35:43	arg1	Characterization					0:15	Characterization	0:15	Characterization of polysaccharide structure in Citrus reticulate 'Chachi'	0:73	Characterization of polysaccharide structure in Citrus reticulate 'Chachi' peel during storage and their bioactivity.					
34274819	10	27	theme	Chachi	1022:1027	arg1	polysaccharides					1029:1043	Chachi polysaccharides	1022:1043	Chachi polysaccharides	1022:1043	The immumomodulatory activities of Chachi polysaccharides were improved as the storage time extended.					
34274819	6	28	dep	-β-D-Galp-	684:693	arg1	→4					681:682	→4	681:682	→4	681:682	NMR data revealed that Chachi polysaccharides were mainly consisted of arabinogalacturonan and another pectin with side chain of →4)-β-D-Galp-(1 → .					
34274819	0	29	theme	polysaccharide	20:33	arg1	structure					35:43	polysaccharide structure	20:43	polysaccharide structure in Citrus reticulate 'Chachi'	20:73	Characterization of polysaccharide structure in Citrus reticulate 'Chachi' peel during storage and their bioactivity.					
34274819	6	30	theme	side	667:670	arg1	chain					672:676	side chain	667:676	side chain of →4)-β-D-Galp-(1 → 	667:698	NMR data revealed that Chachi polysaccharides were mainly consisted of arabinogalacturonan and another pectin with side chain of →4)-β-D-Galp-(1 → .					
34274819	9	31	theme	structure	944:952	arg1	characteristics					954:968	the basic structure characteristics	934:968	the basic structure characteristics	934:968	However, the basic structure characteristics remained stable.					
34274819	4	32	from	Chachi	420:425	arg1	dominant					400:407	dominant	400:407	dominant	400:407	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	12	33	theme	Chachi	1269:1274	arg1	benefits					1257:1264	the enhanced health benefits	1237:1264	the enhanced health benefits of Chachi during storage	1237:1289	The results explained how polysaccharides contributed to the enhanced health benefits of Chachi during storage.					
34274819	6	34	theme	NMR	552:554	arg1	data					556:559	NMR data	552:559	NMR data	552:559	NMR data revealed that Chachi polysaccharides were mainly consisted of arabinogalacturonan and another pectin with side chain of →4)-β-D-Galp-(1 → .					
34274819	11	35	theme	immunomodulatory	1153:1168	arg1	activity					1170:1177	the improved immunomodulatory activity	1140:1177	the improved immunomodulatory activity	1140:1177	The change of molecular weight was responsible for the improved immunomodulatory activity.					
34274819	0	36	from	Chachi	67:72	arg1	Characterization					0:15	Characterization	0:15	Characterization of polysaccharide structure in Citrus reticulate 'Chachi'	0:73	Characterization of polysaccharide structure in Citrus reticulate 'Chachi' peel during storage and their bioactivity.					
34274819	7	37	theme	-α-L-Araf-	718:727	arg1	-β-D-Glcp-					797:806	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→	701:809	-β-D-Glcp-	797:806	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→ were detected.					
34274819	8	38	theme	time	919:922	arg1	extension					898:906	the extension	894:906	the extension of storage time	894:922	The molecular weight of Chachi polysaccharides decreased along with the extension of storage time.					
34274819	4	39	from	dominant	400:407	arg1	Chachi					420:425	Chachi	420:425	Chachi	420:425	As bioactive polysaccharides are dominant in Chachi, Chachi polysaccharides with various storage times (5-20 years) were prepared.					
34274819	12	40	theme	enhanced	1241:1248	arg1	benefits					1257:1264	the enhanced health benefits	1237:1264	the enhanced health benefits of Chachi during storage	1237:1289	The results explained how polysaccharides contributed to the enhanced health benefits of Chachi during storage.					
34274819	8	41	theme	Chachi	850:855	arg1	polysaccharides					857:871	Chachi polysaccharides	850:871	Chachi polysaccharides	850:871	The molecular weight of Chachi polysaccharides decreased along with the extension of storage time.					
34274819	11	42	theme	improved	1144:1151	arg1	activity					1170:1177	the improved immunomodulatory activity	1140:1177	the improved immunomodulatory activity	1140:1177	The change of molecular weight was responsible for the improved immunomodulatory activity.					
34274819	1	43	theme	functional	196:205	arg1	food					207:210	a functional food	194:210	a functional food	194:210	The peel of Citrus reticulate 'Chachiennsis' (Chachi) is widely accepted as a functional food.					
34274819	1	43	theme	functional	196:205	arg1	peel					122:125	The peel	118:125	The peel of Citrus reticulate 'Chachiennsis' (Chachi)	118:170	The peel of Citrus reticulate 'Chachiennsis' (Chachi) is widely accepted as a functional food.					
34274819	7	44	dep	-β-D-Glcp-	797:806	arg1	→4					794:795	→4	794:795	→4	794:795	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→ were detected.					
34274819	7	44	dep	-β-D-Glcp-	797:806	arg1	1→					787:788	1→	787:788	1→	787:788	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→ were detected.					
34274819	1	45	theme	Citrus	130:135	arg1	Chachi					164:169	Chachi	164:169	Chachi	164:169	The peel of Citrus reticulate 'Chachiennsis' (Chachi) is widely accepted as a functional food.					
34274819	1	45	theme	Citrus	130:135	arg1	Chachiennsis					149:160	Citrus reticulate 'Chachiennsis'	130:161	Citrus reticulate 'Chachiennsis' (Chachi)	130:170	The peel of Citrus reticulate 'Chachiennsis' (Chachi) is widely accepted as a functional food.					
34274819	0	46	from	structure	35:43	arg1	Chachi					67:72	Chachi	67:72	Chachi	67:72	Characterization of polysaccharide structure in Citrus reticulate 'Chachi' peel during storage and their bioactivity.					
34274819	12	47	theme	health	1250:1255	arg1	benefits					1257:1264	the enhanced health benefits	1237:1264	the enhanced health benefits of Chachi during storage	1237:1289	The results explained how polysaccharides contributed to the enhanced health benefits of Chachi during storage.					
34274819	8	48	theme	molecular	830:838	arg1	weight					840:845	The molecular weight	826:845	The molecular weight of Chachi polysaccharides	826:871	The molecular weight of Chachi polysaccharides decreased along with the extension of storage time.					
34274819	1	49	theme	reticulate	137:146	arg1	Chachi					164:169	Chachi	164:169	Chachi	164:169	The peel of Citrus reticulate 'Chachiennsis' (Chachi) is widely accepted as a functional food.					
34274819	1	49	theme	reticulate	137:146	arg1	Chachiennsis					149:160	Citrus reticulate 'Chachiennsis'	130:161	Citrus reticulate 'Chachiennsis' (Chachi)	130:170	The peel of Citrus reticulate 'Chachiennsis' (Chachi) is widely accepted as a functional food.					
34274819	7	50	theme	-β-D-Galp-	776:785	arg1	-β-D-Glcp-					797:806	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→	701:809	-β-D-Glcp-	797:806	α-L-Araf-(1→, →5)-α-L-Araf-(1→, →4)-α-D-GalpA-(1→, →4)-α-D-GalpAMe-(1→, →4)-β-D-Galp-(1→ and →4)-β-D-Glcp-(1→ were detected.					
34274819	10	51	theme	storage	1066:1072	arg1	activities					1008:1017	The immumomodulatory activities	987:1017	The immumomodulatory activities of Chachi polysaccharides	987:1043	The immumomodulatory activities of Chachi polysaccharides were improved as the storage time extended.					
34274819	10	51	theme	storage	1066:1072	arg1	time					1074:1077	the storage time	1062:1077	the storage time extended	1062:1086	The immumomodulatory activities of Chachi polysaccharides were improved as the storage time extended.					
31982522	3	0	theme	unexpected	715:724	arg1	hydrophobicity					726:739	an unexpected hydrophobicity	712:739	an unexpected hydrophobicity	712:739	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	2	1	theme	hemicelluloses	301:314	arg1	contents					316:323	hemicelluloses contents	301:323	hemicelluloses contents	301:323	This LMM treatment affected both lignin and hemicelluloses contents and modified the fibers properties identified as crucial parameters in an industrial context, i.e. coloration, wettability, crystallinity, fibers diameter and chemical composition.					
31982522	3	2	theme	enhancement	696:706	arg1	responsible					653:663	responsible	653:663	responsible	653:663	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	2	3	theme	fibers	464:469	arg1	diameter					471:478	fibers diameter	464:478	fibers diameter	464:478	This LMM treatment affected both lignin and hemicelluloses contents and modified the fibers properties identified as crucial parameters in an industrial context, i.e. coloration, wettability, crystallinity, fibers diameter and chemical composition.					
31982522	0	4	theme	flax	89:92	arg1	fibers					99:104	lignocellulosic flax bast fibers	73:104	lignocellulosic flax bast fibers	73:104	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.					
31982522	0	5	from	Effect	0:5	arg1	properties					59:68	the surface properties	47:68	the surface properties of lignocellulosic flax bast fibers	47:104	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.					
31982522	3	6	theme	Surface	506:512	arg1	chemistry					514:522	Surface chemistry	506:522	Surface chemistry of the fibers	506:536	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	0	7	theme	lignocellulosic	73:87	arg1	fibers					99:104	lignocellulosic flax bast fibers	73:104	lignocellulosic flax bast fibers	73:104	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.					
31982522	2	8	dep	coloration	424:433	arg1	i.e.					419:422	i.e.	419:422	i.e.	419:422	This LMM treatment affected both lignin and hemicelluloses contents and modified the fibers properties identified as crucial parameters in an industrial context, i.e. coloration, wettability, crystallinity, fibers diameter and chemical composition.					
31982522	1	9	theme	lactic	223:228	arg1	d-glucose					236:244	d-glucose	236:244	d-glucose	236:244	Bast flax fibers were treated, with or without ultrasound assistance, using a low melting mixture (LMM) composed of lactic acid, d-glucose and water.					
31982522	1	9	theme	lactic	223:228	arg1	acid					230:233	lactic acid	223:233	lactic acid	223:233	Bast flax fibers were treated, with or without ultrasound assistance, using a low melting mixture (LMM) composed of lactic acid, d-glucose and water.					
31982522	1	9	theme	lactic	223:228	arg1	water					250:254	water	250:254	water	250:254	Bast flax fibers were treated, with or without ultrasound assistance, using a low melting mixture (LMM) composed of lactic acid, d-glucose and water.					
31982522	0	10	theme	fibers	99:104	arg1	properties					59:68	the surface properties	47:68	the surface properties of lignocellulosic flax bast fibers	47:104	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.					
31982522	4	11	theme	treatments	854:863	arg1	independent					835:845	independent	835:845	independent	835:845	It has been found that LMM treatments bleach fibers, which is considered a beneficial effect independent of the treatments.					
31982522	0	12	theme	bast	94:97	arg1	fibers					99:104	lignocellulosic flax bast fibers	73:104	lignocellulosic flax bast fibers	73:104	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.					
31982522	3	13	theme	hydrophobicity	726:739	arg1	responsible					653:663	responsible	653:663	responsible	653:663	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	2	14	theme	fibers	342:347	arg1	properties					349:358	the fibers properties	338:358	the fibers properties identified as crucial parameters in an industrial context, i.e. coloration, wettability, crystallinity, fibers diameter and chemical composition	338:503	This LMM treatment affected both lignin and hemicelluloses contents and modified the fibers properties identified as crucial parameters in an industrial context, i.e. coloration, wettability, crystallinity, fibers diameter and chemical composition.					
31982522	2	15	theme	LMM	262:264	arg1	treatment					266:274	This LMM treatment	257:274	This LMM treatment	257:274	This LMM treatment affected both lignin and hemicelluloses contents and modified the fibers properties identified as crucial parameters in an industrial context, i.e. coloration, wettability, crystallinity, fibers diameter and chemical composition.					
31982522	1	16	theme	ultrasound	154:163	arg1	assistance					165:174	ultrasound assistance	154:174	ultrasound assistance	154:174	Bast flax fibers were treated, with or without ultrasound assistance, using a low melting mixture (LMM) composed of lactic acid, d-glucose and water.					
31982522	2	17	theme	industrial	399:408	arg1	context					410:416	an industrial context	396:416	an industrial context	396:416	This LMM treatment affected both lignin and hemicelluloses contents and modified the fibers properties identified as crucial parameters in an industrial context, i.e. coloration, wettability, crystallinity, fibers diameter and chemical composition.					
31982522	0	18	theme	low	12:14	arg1	mixture					36:42	a low melting temperature mixture	10:42	a low melting temperature mixture	10:42	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.					
31982522	4	19	theme	LMM	765:767	arg1	treatments					769:778	LMM treatments	765:778	LMM treatments	765:778	It has been found that LMM treatments bleach fibers, which is considered a beneficial effect independent of the treatments.					
31982522	3	20	theme	responsible	653:663	arg1	rearrangements					638:651	macromolecular rearrangements	623:651	macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity	623:739	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	2	21	theme	crucial	374:380	arg1	parameters					382:391	crucial parameters	374:391	crucial parameters	374:391	This LMM treatment affected both lignin and hemicelluloses contents and modified the fibers properties identified as crucial parameters in an industrial context, i.e. coloration, wettability, crystallinity, fibers diameter and chemical composition.					
31982522	0	22	theme	temperature	24:34	arg1	mixture					36:42	a low melting temperature mixture	10:42	a low melting temperature mixture	10:42	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.					
31982522	3	23	theme	fluorescent	564:574	arg1	modules					605:611	fluorescent tagged carbohydrates binding modules	564:611	fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity	564:739	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	0	24	theme	melting	16:22	arg1	mixture					36:42	a low melting temperature mixture	10:42	a low melting temperature mixture	10:42	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.					
31982522	3	25	theme	macromolecular	623:636	arg1	rearrangements					638:651	macromolecular rearrangements	623:651	macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity	623:739	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	3	26	theme	tagged	576:581	arg1	modules					605:611	fluorescent tagged carbohydrates binding modules	564:611	fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity	564:739	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	1	27	theme	low	185:187	arg1	LMM					206:208	LMM	206:208	LMM	206:208	Bast flax fibers were treated, with or without ultrasound assistance, using a low melting mixture (LMM) composed of lactic acid, d-glucose and water.					
31982522	1	27	theme	low	185:187	arg1	mixture					197:203	a low melting mixture	183:203	a low melting mixture (LMM) composed of lactic acid, d-glucose and water	183:254	Bast flax fibers were treated, with or without ultrasound assistance, using a low melting mixture (LMM) composed of lactic acid, d-glucose and water.					
31982522	2	28	theme	chemical	484:491	arg1	composition					493:503	chemical composition	484:503	chemical composition	484:503	This LMM treatment affected both lignin and hemicelluloses contents and modified the fibers properties identified as crucial parameters in an industrial context, i.e. coloration, wettability, crystallinity, fibers diameter and chemical composition.					
31982522	0	29	theme	mixture	36:42	arg1	Effect					0:5	Effect	0:5	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.	0:105	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.					
31982522	3	30	theme	fibers	675:680	arg1	enhancement					696:706	a fibers crystallinity enhancement	673:706	a fibers crystallinity enhancement	673:706	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	4	31	theme	independent	835:845	arg1	effect					828:833	a beneficial effect	815:833	a beneficial effect independent of the treatments	815:863	It has been found that LMM treatments bleach fibers, which is considered a beneficial effect independent of the treatments.					
31982522	1	32	theme	Bast	107:110	arg1	fibers					117:122	Bast flax fibers	107:122	Bast flax fibers	107:122	Bast flax fibers were treated, with or without ultrasound assistance, using a low melting mixture (LMM) composed of lactic acid, d-glucose and water.					
31982522	3	33	theme	fibers	531:536	arg1	chemistry					514:522	Surface chemistry	506:522	Surface chemistry of the fibers	506:536	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	3	34	theme	crystallinity	682:694	arg1	enhancement					696:706	a fibers crystallinity enhancement	673:706	a fibers crystallinity enhancement	673:706	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	4	35	theme	beneficial	817:826	arg1	effect					828:833	a beneficial effect	815:833	a beneficial effect independent of the treatments	815:863	It has been found that LMM treatments bleach fibers, which is considered a beneficial effect independent of the treatments.					
31982522	1	36	theme	flax	112:115	arg1	fibers					117:122	Bast flax fibers	107:122	Bast flax fibers	107:122	Bast flax fibers were treated, with or without ultrasound assistance, using a low melting mixture (LMM) composed of lactic acid, d-glucose and water.					
31982522	1	37	theme	melting	189:195	arg1	LMM					206:208	LMM	206:208	LMM	206:208	Bast flax fibers were treated, with or without ultrasound assistance, using a low melting mixture (LMM) composed of lactic acid, d-glucose and water.					
31982522	1	37	theme	melting	189:195	arg1	mixture					197:203	a low melting mixture	183:203	a low melting mixture (LMM) composed of lactic acid, d-glucose and water	183:254	Bast flax fibers were treated, with or without ultrasound assistance, using a low melting mixture (LMM) composed of lactic acid, d-glucose and water.					
31982522	0	38	theme	surface	51:57	arg1	properties					59:68	the surface properties	47:68	the surface properties of lignocellulosic flax bast fibers	47:104	Effect of a low melting temperature mixture on the surface properties of lignocellulosic flax bast fibers.					
31982522	3	39	theme	carbohydrates	583:595	arg1	modules					605:611	fluorescent tagged carbohydrates binding modules	564:611	fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity	564:739	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	3	40	theme	binding	597:603	arg1	modules					605:611	fluorescent tagged carbohydrates binding modules	564:611	fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity	564:739	Surface chemistry of the fibers were investigated through fluorescent tagged carbohydrates binding modules revealing macromolecular rearrangements responsible of both a fibers crystallinity enhancement and an unexpected hydrophobicity.					
31982522	2	41	from	parameters	382:391	arg1	context					410:416	an industrial context	396:416	an industrial context	396:416	This LMM treatment affected both lignin and hemicelluloses contents and modified the fibers properties identified as crucial parameters in an industrial context, i.e. coloration, wettability, crystallinity, fibers diameter and chemical composition.					
33581437	6	0	theme	distinct	1093:1100	arg1	characteristics					1102:1116	distinct characteristics	1093:1116	distinct characteristics that impart favorable properties to various foods and bioactive delivery systems	1093:1197	This study provides insights into the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems.					
33581437	1	1	theme	termed	250:255	arg1	"					263:263	termed "ZGPEs"	250:263	termed "ZGPEs"	250:263	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	1	1	theme	termed	250:255	arg1	emulsions					239:247	Pickering emulsions	229:247	Pickering emulsions (termed "ZGPEs")	229:264	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	0	2	theme	emulsion	102:109	arg1	gels					111:114	Pickering emulsion gels	92:114	Pickering emulsion gels with favorable properties	92:140	Exploiting the robust network structure of zein/low-acyl gellan gum nanocomplexes to create Pickering emulsion gels with favorable properties.					
33581437	4	3	theme	Pickering	781:789	arg1	emulsions					791:799	other previously reported zein-polysaccharide-based Pickering emulsions	729:799	other previously reported zein-polysaccharide-based Pickering emulsions	729:799	The results showed that ZGPEs exhibited robust colloidal properties and distinct advantage over other previously reported zein-polysaccharide-based Pickering emulsions.					
33581437	3	4	theme	electron	504:511	arg1	cryo-SEM					525:532	cryo-SEM	525:532	cryo-SEM	525:532	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	4	theme	electron	504:511	arg1	microscopy					513:522	cryo-scanning electron microscopy	490:522	cryo-scanning electron microscopy (cryo-SEM)	490:533	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	5	theme	GG	367:368	arg1	concentration					370:382	GG concentration	367:382	GG concentration	367:382	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	0	6	theme	Pickering	92:100	arg1	gels					111:114	Pickering emulsion gels	92:114	Pickering emulsion gels with favorable properties	92:140	Exploiting the robust network structure of zein/low-acyl gellan gum nanocomplexes to create Pickering emulsion gels with favorable properties.					
33581437	6	7	theme	bioactive	1172:1180	arg1	systems					1191:1197	bioactive delivery systems	1172:1197	bioactive delivery systems	1172:1197	This study provides insights into the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems.					
33581437	4	8	theme	other	729:733	arg1	emulsions					791:799	other previously reported zein-polysaccharide-based Pickering emulsions	729:799	other previously reported zein-polysaccharide-based Pickering emulsions	729:799	The results showed that ZGPEs exhibited robust colloidal properties and distinct advantage over other previously reported zein-polysaccharide-based Pickering emulsions.					
33581437	1	9	theme	ZGPEs	258:262	arg1	"					263:263	termed "ZGPEs"	250:263	termed "ZGPEs"	250:263	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	1	9	theme	ZGPEs	258:262	arg1	emulsions					239:247	Pickering emulsions	229:247	Pickering emulsions (termed "ZGPEs")	229:264	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	4	10	theme	robust	673:678	arg1	properties					690:699	robust colloidal properties	673:699	robust colloidal properties	673:699	The results showed that ZGPEs exhibited robust colloidal properties and distinct advantage over other previously reported zein-polysaccharide-based Pickering emulsions.					
33581437	4	11	theme	zein-polysaccharide-based	755:779	arg1	emulsions					791:799	other previously reported zein-polysaccharide-based Pickering emulsions	729:799	other previously reported zein-polysaccharide-based Pickering emulsions	729:799	The results showed that ZGPEs exhibited robust colloidal properties and distinct advantage over other previously reported zein-polysaccharide-based Pickering emulsions.					
33581437	6	12	theme	Pickering	1068:1076	arg1	emulsions					1078:1086	food-grade Pickering emulsions	1057:1086	food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems	1057:1197	This study provides insights into the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems.					
33581437	3	13	theme	pH	402:403	arg1	effects					356:362	The effects	352:362	The effects of GG concentration, oil fraction and pH on ZGPEs	352:412	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	14	theme	concentration	370:382	arg1	effects					356:362	The effects	352:362	The effects of GG concentration, oil fraction and pH on ZGPEs	352:412	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	15	from	effects	356:362	arg1	ZGPEs					408:412	ZGPEs	408:412	ZGPEs	408:412	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	5	16	dep	CLSM	802:805	arg1	analyses					826:833	analyses	826:833	analyses	826:833	CLSM, STED and cryo-SEM analyses revealed that the network structures formed by GG and ZGPs at the continuous phase and oil-water interface were the main contributors to the emulsion's characteristics.					
33581437	3	17	theme	cryo-scanning	490:502	arg1	cryo-SEM					525:532	cryo-SEM	525:532	cryo-SEM	525:532	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	17	theme	cryo-scanning	490:502	arg1	microscopy					513:522	cryo-scanning electron microscopy	490:522	cryo-scanning electron microscopy (cryo-SEM)	490:533	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	1	18	theme	composite	173:181	arg1	particles					183:191	Zein/low-acyl gellan gum (GG) composite particles	143:191	Zein/low-acyl gellan gum (GG) composite particles (ZGPs)	143:198	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	1	18	theme	composite	173:181	arg1	ZGPs					194:197	ZGPs	194:197	ZGPs	194:197	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	6	19	theme	delivery	1182:1189	arg1	systems					1191:1197	bioactive delivery systems	1172:1197	bioactive delivery systems	1172:1197	This study provides insights into the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems.					
33581437	0	20	with	gels	111:114	arg1	properties					131:140	favorable properties	121:140	favorable properties	121:140	Exploiting the robust network structure of zein/low-acyl gellan gum nanocomplexes to create Pickering emulsion gels with favorable properties.					
33581437	4	21	theme	distinct	705:712	arg1	advantage					714:722	distinct advantage	705:722	distinct advantage	705:722	The results showed that ZGPEs exhibited robust colloidal properties and distinct advantage over other previously reported zein-polysaccharide-based Pickering emulsions.					
33581437	3	22	theme	fraction	389:396	arg1	effects					356:362	The effects	352:362	The effects of GG concentration, oil fraction and pH on ZGPEs	352:412	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	4	23	theme	colloidal	680:688	arg1	properties					690:699	robust colloidal properties	673:699	robust colloidal properties	673:699	The results showed that ZGPEs exhibited robust colloidal properties and distinct advantage over other previously reported zein-polysaccharide-based Pickering emulsions.					
33581437	5	24	theme	network	853:859	arg1	structures					861:870	the network structures	849:870	the network structures formed by GG and ZGPs at the continuous phase and oil-water interface	849:940	CLSM, STED and cryo-SEM analyses revealed that the network structures formed by GG and ZGPs at the continuous phase and oil-water interface were the main contributors to the emulsion's characteristics.					
33581437	5	24	theme	network	853:859	arg1	contributors					956:967	the main contributors	947:967	the main contributors to the emulsion's characteristics	947:1001	CLSM, STED and cryo-SEM analyses revealed that the network structures formed by GG and ZGPs at the continuous phase and oil-water interface were the main contributors to the emulsion's characteristics.					
33581437	2	25	theme	ZGPs	286:289	arg1	wettability					271:281	The wettability	267:281	The wettability of ZGPs	267:289	The wettability of ZGPs was manipulated simply by adjusting the concentration of GG.					
33581437	6	26	theme	food-grade	1057:1066	arg1	emulsions					1078:1086	food-grade Pickering emulsions	1057:1086	food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems	1057:1197	This study provides insights into the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems.					
33581437	0	27	theme	network	22:28	arg1	structure					30:38	the robust network structure	11:38	the robust network structure of zein/low-acyl gellan gum	11:66	Exploiting the robust network structure of zein/low-acyl gellan gum nanocomplexes to create Pickering emulsion gels with favorable properties.					
33581437	6	28	theme	emulsions	1078:1086	arg1	fabrication					1042:1052	the fabrication	1038:1052	the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems	1038:1197	This study provides insights into the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems.					
33581437	5	29	theme	main	951:954	arg1	contributors					956:967	the main contributors	947:967	the main contributors to the emulsion's characteristics	947:1001	CLSM, STED and cryo-SEM analyses revealed that the network structures formed by GG and ZGPs at the continuous phase and oil-water interface were the main contributors to the emulsion's characteristics.					
33581437	5	29	theme	main	951:954	arg1	structures					861:870	the network structures	849:870	the network structures formed by GG and ZGPs at the continuous phase and oil-water interface	849:940	CLSM, STED and cryo-SEM analyses revealed that the network structures formed by GG and ZGPs at the continuous phase and oil-water interface were the main contributors to the emulsion's characteristics.					
33581437	0	30	theme	robust	15:20	arg1	structure					30:38	the robust network structure	11:38	the robust network structure of zein/low-acyl gellan gum	11:66	Exploiting the robust network structure of zein/low-acyl gellan gum nanocomplexes to create Pickering emulsion gels with favorable properties.					
33581437	0	31	theme	favorable	121:129	arg1	properties					131:140	favorable properties	121:140	favorable properties	121:140	Exploiting the robust network structure of zein/low-acyl gellan gum nanocomplexes to create Pickering emulsion gels with favorable properties.					
33581437	1	32	theme	gellan	157:162	arg1	gum					164:166	Zein/low-acyl gellan gum	143:166	Zein/low-acyl gellan gum (GG) composite particles (ZGPs)	143:198	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	1	32	theme	gellan	157:162	arg1	GG					169:170	GG	169:170	GG	169:170	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	5	33	theme	continuous	901:910	arg1	phase					912:916	the continuous phase	897:916	the continuous phase	897:916	CLSM, STED and cryo-SEM analyses revealed that the network structures formed by GG and ZGPs at the continuous phase and oil-water interface were the main contributors to the emulsion's characteristics.					
33581437	3	34	theme	scattering	550:559	arg1	technique					561:569	dynamic light scattering technique	536:569	dynamic light scattering technique	536:569	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	35	theme	oil	385:387	arg1	fraction					389:396	oil fraction	385:396	oil fraction	385:396	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	0	36	theme	gellan	57:62	arg1	gum					64:66	zein/low-acyl gellan gum	43:66	zein/low-acyl gellan gum	43:66	Exploiting the robust network structure of zein/low-acyl gellan gum nanocomplexes to create Pickering emulsion gels with favorable properties.					
33581437	6	37	with	emulsions	1078:1086	arg1	characteristics					1102:1116	distinct characteristics	1093:1116	distinct characteristics that impart favorable properties to various foods and bioactive delivery systems	1093:1197	This study provides insights into the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems.					
33581437	3	38	theme	dynamic	536:542	arg1	technique					561:569	dynamic light scattering technique	536:569	dynamic light scattering technique	536:569	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	39	theme	laser	456:460	arg1	CLSM					483:486	CLSM	483:486	CLSM	483:486	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	39	theme	laser	456:460	arg1	microscopy					471:480	confocal laser scanning microscopy	447:480	confocal laser scanning microscopy (CLSM)	447:487	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	0	40	theme	zein/low-acyl	43:55	arg1	gum					64:66	zein/low-acyl gellan gum	43:66	zein/low-acyl gellan gum	43:66	Exploiting the robust network structure of zein/low-acyl gellan gum nanocomplexes to create Pickering emulsion gels with favorable properties.					
33581437	3	41	theme	light	544:548	arg1	technique					561:569	dynamic light scattering technique	536:569	dynamic light scattering technique	536:569	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	42	theme	scanning	462:469	arg1	CLSM					483:486	CLSM	483:486	CLSM	483:486	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	42	theme	scanning	462:469	arg1	microscopy					471:480	confocal laser scanning microscopy	447:480	confocal laser scanning microscopy (CLSM)	447:487	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	5	43	theme	oil-water	922:930	arg1	interface					932:940	oil-water interface	922:940	oil-water interface	922:940	CLSM, STED and cryo-SEM analyses revealed that the network structures formed by GG and ZGPs at the continuous phase and oil-water interface were the main contributors to the emulsion's characteristics.					
33581437	3	44	theme	emission	583:590	arg1	STED					603:606	STED	603:606	STED	603:606	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	44	theme	emission	583:590	arg1	depletion					592:600	emission depletion	583:600	stimulated emission depletion (STED) nanoscopy	572:617	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	6	45	theme	favorable	1130:1138	arg1	properties					1140:1149	favorable properties	1130:1149	favorable properties	1130:1149	This study provides insights into the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems.					
33581437	4	46	theme	reported	746:753	arg1	emulsions					791:799	other previously reported zein-polysaccharide-based Pickering emulsions	729:799	other previously reported zein-polysaccharide-based Pickering emulsions	729:799	The results showed that ZGPEs exhibited robust colloidal properties and distinct advantage over other previously reported zein-polysaccharide-based Pickering emulsions.					
33581437	3	47	theme	depletion	592:600	arg1	nanoscopy					609:617	stimulated emission depletion (STED) nanoscopy	572:617	stimulated emission depletion (STED) nanoscopy	572:617	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	1	48	theme	Zein/low-acyl	143:155	arg1	gum					164:166	Zein/low-acyl gellan gum	143:166	Zein/low-acyl gellan gum (GG) composite particles (ZGPs)	143:198	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	1	48	theme	Zein/low-acyl	143:155	arg1	GG					169:170	GG	169:170	GG	169:170	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	6	49	theme	various	1154:1160	arg1	foods					1162:1166	various foods	1154:1166	various foods	1154:1166	This study provides insights into the fabrication of food-grade Pickering emulsions with distinct characteristics that impart favorable properties to various foods and bioactive delivery systems.					
33581437	2	50	theme	GG	348:349	arg1	concentration					331:343	the concentration	327:343	the concentration of GG	327:349	The wettability of ZGPs was manipulated simply by adjusting the concentration of GG.					
33581437	3	51	theme	confocal	447:454	arg1	CLSM					483:486	CLSM	483:486	CLSM	483:486	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	3	51	theme	confocal	447:454	arg1	microscopy					471:480	confocal laser scanning microscopy	447:480	confocal laser scanning microscopy (CLSM)	447:487	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	1	52	theme	Pickering	229:237	arg1	"					263:263	termed "ZGPEs"	250:263	termed "ZGPEs"	250:263	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	1	52	theme	Pickering	229:237	arg1	emulsions					239:247	Pickering emulsions	229:247	Pickering emulsions (termed "ZGPEs")	229:264	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	0	53	theme	gum	64:66	arg1	structure					30:38	the robust network structure	11:38	the robust network structure of zein/low-acyl gellan gum	11:66	Exploiting the robust network structure of zein/low-acyl gellan gum nanocomplexes to create Pickering emulsion gels with favorable properties.					
33581437	3	54	theme	stimulated	572:581	arg1	nanoscopy					609:617	stimulated emission depletion (STED) nanoscopy	572:617	stimulated emission depletion (STED) nanoscopy	572:617	The effects of GG concentration, oil fraction and pH on ZGPEs were systematically evaluated by confocal laser scanning microscopy (CLSM), cryo-scanning electron microscopy (cryo-SEM), dynamic light scattering technique, stimulated emission depletion (STED) nanoscopy and rheology.					
33581437	1	55	theme	gum	164:166	arg1	particles					183:191	Zein/low-acyl gellan gum (GG) composite particles	143:191	Zein/low-acyl gellan gum (GG) composite particles (ZGPs)	143:198	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
33581437	1	55	theme	gum	164:166	arg1	ZGPs					194:197	ZGPs	194:197	ZGPs	194:197	Zein/low-acyl gellan gum (GG) composite particles (ZGPs) were fabricated to stabilize Pickering emulsions (termed "ZGPEs").					
32580048	2	0	theme	bagasse	459:465	arg1	size					467:470	the optimum bagasse size	447:470	the optimum bagasse size	447:470	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	4	1	theme	Fe	908:909	arg1	/SCB3					911:915	the as prepared MIL-101(Fe)/SCB3	884:915	the as prepared MIL-101(Fe)/SCB3	884:915	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	1	2	theme	Fe	133:134	arg1	SCB					156:158	SCB	156:158	SCB	156:158	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	1	2	theme	Fe	133:134	arg1	bagasse					147:153	MIL-101(Fe)/sugarcane bagasse	125:153	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate	125:222	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	2	3	from	Effects	271:277	arg1	morphology					302:311	morphology	302:311	morphology	302:311	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	2	3	from	Effects	271:277	arg1	performances					317:328	performances	317:328	performances	317:328	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	3	4	theme	charged	739:745	arg1	surface					747:753	the more positively charged surface	719:753	the more positively charged surface	719:753	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	0	5	theme	Fe	64:65	arg1	composite					76:84	MIL-101(Fe)/bagasse composite	56:84	MIL-101(Fe)/bagasse composite	56:84	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite prepared through bagasse size control.					
32580048	3	6	theme	blank	627:631	arg1	Fe					641:642	Fe	641:642	Fe	641:642	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	6	theme	blank	627:631	arg1	MIL-101					633:639	blank MIL-101	627:639	blank MIL-101(Fe)	627:643	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	7	theme	adsorption	776:785	arg1	FeOHx					810:814	FeOHx	810:814	FeOHx	810:814	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	7	theme	adsorption	776:785	arg1	Cl-					833:835	exchangeable Cl-	820:835	exchangeable Cl-	820:835	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	7	theme	adsorption	776:785	arg1	sites					794:798	the more exposed adsorption active sites	759:798	the more exposed adsorption active sites including FeOHx and exchangeable Cl-	759:835	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	5	8	dep	10.0	1162:1165	arg1	to					1159:1160	to	1159:1160	to	1159:1160	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3 was from 3.0 to 10.0, and in this range Fe release was less than 0.03%.					
32580048	5	8	dep	10.0	1162:1165	arg1	%					1215:1215	less than 0.03%	1201:1215	less than 0.03%	1201:1215	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3 was from 3.0 to 10.0, and in this range Fe release was less than 0.03%.					
32580048	4	9	theme	adsorption	1050:1059	arg1	capacity					1061:1068	phosphate adsorption capacity	1040:1068	phosphate adsorption capacity	1040:1068	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	3	10	theme	active	787:792	arg1	FeOHx					810:814	FeOHx	810:814	FeOHx	810:814	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	10	theme	active	787:792	arg1	Cl-					833:835	exchangeable Cl-	820:835	exchangeable Cl-	820:835	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	10	theme	active	787:792	arg1	sites					794:798	the more exposed adsorption active sites	759:798	the more exposed adsorption active sites including FeOHx and exchangeable Cl-	759:835	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	5	11	theme	MIL-101	1129:1135	arg1	/SCB3					1140:1144	MIL-101(Fe)/SCB3	1129:1144	MIL-101(Fe)/SCB3	1129:1144	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3 was from 3.0 to 10.0, and in this range Fe release was less than 0.03%.					
32580048	0	12	theme	/bagasse	67:74	arg1	composite					76:84	MIL-101(Fe)/bagasse composite	56:84	MIL-101(Fe)/bagasse composite	56:84	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite prepared through bagasse size control.					
32580048	4	13	theme	common	975:980	arg1	ions					1003:1006	the common cationic and anionic ions	971:1006	the common cationic and anionic ions	971:1006	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	3	14	theme	exchangeable	820:831	arg1	Cl-					833:835	exchangeable Cl-	820:835	exchangeable Cl-	820:835	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	6	15	theme	Adsorption	1218:1227	arg1	mechanism					1229:1237	Adsorption mechanism	1218:1237	Adsorption mechanism	1218:1237	Adsorption mechanism showed that phosphate was adsorbed mainly through electrostatic force, ion-exchange, and inner-sphere surface complex.					
32580048	0	16	from	solution	44:51	arg1	removal					10:16	Selective removal	0:16	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite	0:84	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite prepared through bagasse size control.					
32580048	4	17	from	effects	1029:1035	arg1	rate					1074:1077	rate	1074:1077	rate	1074:1077	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	4	17	from	effects	1029:1035	arg1	capacity					1061:1068	phosphate adsorption capacity	1040:1068	phosphate adsorption capacity	1040:1068	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	4	18	theme	phosphate	1040:1048	arg1	capacity					1061:1068	phosphate adsorption capacity	1040:1068	phosphate adsorption capacity	1040:1068	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	1	19	theme	/sugarcane	136:145	arg1	SCB					156:158	SCB	156:158	SCB	156:158	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	1	19	theme	/sugarcane	136:145	arg1	bagasse					147:153	MIL-101(Fe)/sugarcane bagasse	125:153	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate	125:222	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	4	20	theme	adsorption	929:938	arg1	affinity					940:947	high adsorption affinity	924:947	high adsorption affinity toward phosphate	924:964	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	2	21	theme	optimum	451:457	arg1	size					467:470	the optimum bagasse size	447:470	the optimum bagasse size	447:470	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	5	22	theme	pH	1092:1093	arg1	range					1095:1099	The optimum pH range	1080:1099	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3	1080:1144	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3 was from 3.0 to 10.0, and in this range Fe release was less than 0.03%.					
32580048	2	23	theme	size	290:293	arg1	Effects					271:277	Effects	271:277	Effects of bagasse size on the morphology and performances of the composites	271:346	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	0	24	theme	Selective	0:8	arg1	removal					10:16	Selective removal	0:16	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite	0:84	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite prepared through bagasse size control.					
32580048	1	25	theme	in-situ	245:251	arg1	method					263:268	in-situ synthesis method	245:268	in-situ synthesis method	245:268	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	6	26	theme	surface	1341:1347	arg1	complex					1349:1355	inner-sphere surface complex	1328:1355	inner-sphere surface complex	1328:1355	Adsorption mechanism showed that phosphate was adsorbed mainly through electrostatic force, ion-exchange, and inner-sphere surface complex.					
32580048	2	27	theme	bagasse	282:288	arg1	size					290:293	bagasse size	282:293	bagasse size	282:293	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	4	28	theme	negligible	1018:1027	arg1	effects					1029:1035	negligible effects	1018:1035	negligible effects on phosphate adsorption capacity and rate	1018:1077	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	0	29	theme	phosphate	21:29	arg1	removal					10:16	Selective removal	0:16	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite	0:84	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite prepared through bagasse size control.					
32580048	1	30	theme	synthesis	253:261	arg1	method					263:268	in-situ synthesis method	245:268	in-situ synthesis method	245:268	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	0	31	theme	size	111:114	arg1	control					116:122	bagasse size control	103:122	bagasse size control	103:122	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite prepared through bagasse size control.					
32580048	3	32	theme	bagasse	530:536	arg1	size					538:541	bagasse size	530:541	bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3)	530:576	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	5	33	theme	Fe	1186:1187	arg1	release					1189:1195	this range Fe release	1175:1195	this range Fe release	1175:1195	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3 was from 3.0 to 10.0, and in this range Fe release was less than 0.03%.					
32580048	0	34	theme	bagasse	103:109	arg1	control					116:122	bagasse size control	103:122	bagasse size control	103:122	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite prepared through bagasse size control.					
32580048	3	35	theme	higher	590:595	arg1	capacity					608:615	much higher adsorption capacity	585:615	much higher adsorption capacity	585:615	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	2	36	dep	morphology	302:311	arg1	the					298:300	the	298:300	the	298:300	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	2	37	theme	composites	337:346	arg1	morphology					302:311	morphology	302:311	morphology	302:311	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	2	37	theme	composites	337:346	arg1	performances					317:328	performances	317:328	performances	317:328	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	0	38	theme	aqueous	36:42	arg1	solution					44:51	aqueous solution	36:51	aqueous solution	36:51	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite prepared through bagasse size control.					
32580048	5	39	theme	range	1180:1184	arg1	release					1189:1195	this range Fe release	1175:1195	this range Fe release	1175:1195	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3 was from 3.0 to 10.0, and in this range Fe release was less than 0.03%.					
32580048	5	40	from	adsorption	1115:1124	arg1	/SCB3					1140:1144	MIL-101(Fe)/SCB3	1129:1144	MIL-101(Fe)/SCB3	1129:1144	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3 was from 3.0 to 10.0, and in this range Fe release was less than 0.03%.					
32580048	3	41	theme	adsorption	597:606	arg1	capacity					608:615	much higher adsorption capacity	585:615	much higher adsorption capacity	585:615	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	42	theme	MIL-101	560:566	arg1	mesh					554:557	200-300 mesh	546:557	200-300 mesh (MIL-101(Fe)/SCB3)	546:576	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	42	theme	MIL-101	560:566	arg1	/SCB3					571:575	MIL-101(Fe)/SCB3	560:575	MIL-101(Fe)/SCB3	560:575	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	4	43	theme	anionic	995:1001	arg1	ions					1003:1006	the common cationic and anionic ions	971:1006	the common cationic and anionic ions	971:1006	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	1	44	theme	high	166:169	arg1	capacity					182:189	high adsorption capacity	166:189	high adsorption capacity	166:189	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	6	45	theme	electrostatic	1289:1301	arg1	force					1303:1307	electrostatic force	1289:1307	electrostatic force	1289:1307	Adsorption mechanism showed that phosphate was adsorbed mainly through electrostatic force, ion-exchange, and inner-sphere surface complex.					
32580048	7	46	theme	Simulated	1358:1366	arg1	experiment					1389:1398	Simulated wastewater treatment experiment	1358:1398	Simulated wastewater treatment experiment	1358:1398	Simulated wastewater treatment experiment showed that MIL-101(Fe)/SCB3 could efficiently remove phosphate from aqueous solution.					
32580048	4	47	theme	experimental	846:857	arg1	results					859:865	Co-ions experimental results	838:865	Co-ions experimental results	838:865	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	7	48	theme	Fe	1420:1421	arg1	/SCB3					1423:1427	MIL-101(Fe)/SCB3	1412:1427	MIL-101(Fe)/SCB3	1412:1427	Simulated wastewater treatment experiment showed that MIL-101(Fe)/SCB3 could efficiently remove phosphate from aqueous solution.					
32580048	2	49	theme	phosphate	408:416	arg1	behavior					382:389	adsorption behavior	371:389	adsorption behavior	371:389	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	2	49	theme	phosphate	408:416	arg1	mechanism					395:403	mechanism	395:403	mechanism	395:403	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	5	50	theme	phosphate	1105:1113	arg1	adsorption					1115:1124	phosphate adsorption	1105:1124	phosphate adsorption on MIL-101(Fe)/SCB3	1105:1144	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3 was from 3.0 to 10.0, and in this range Fe release was less than 0.03%.					
32580048	1	51	theme	adsorption	171:180	arg1	capacity					182:189	high adsorption capacity	166:189	high adsorption capacity	166:189	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	4	52	theme	high	924:927	arg1	affinity					940:947	high adsorption affinity	924:947	high adsorption affinity toward phosphate	924:964	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	3	53	theme	other	682:686	arg1	size					696:699	the other bagasse size	678:699	the other bagasse size	678:699	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	53	theme	other	682:686	arg1	due					712:714	due	712:714	due	712:714	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	54	theme	mesh	554:557	arg1	size					538:541	bagasse size	530:541	bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3)	530:576	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	7	55	theme	wastewater	1368:1377	arg1	experiment					1389:1398	Simulated wastewater treatment experiment	1358:1398	Simulated wastewater treatment experiment	1358:1398	Simulated wastewater treatment experiment showed that MIL-101(Fe)/SCB3 could efficiently remove phosphate from aqueous solution.					
32580048	3	56	theme	bagasse	688:694	arg1	size					696:699	the other bagasse size	678:699	the other bagasse size	678:699	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	56	theme	bagasse	688:694	arg1	due					712:714	due	712:714	due	712:714	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	4	57	theme	Co-ions	838:844	arg1	results					859:865	Co-ions experimental results	838:865	Co-ions experimental results	838:865	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	7	58	theme	MIL-101	1412:1418	arg1	/SCB3					1423:1427	MIL-101(Fe)/SCB3	1412:1427	MIL-101(Fe)/SCB3	1412:1427	Simulated wastewater treatment experiment showed that MIL-101(Fe)/SCB3 could efficiently remove phosphate from aqueous solution.					
32580048	4	59	theme	prepared	891:898	arg1	/SCB3					911:915	the as prepared MIL-101(Fe)/SCB3	884:915	the as prepared MIL-101(Fe)/SCB3	884:915	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	6	60	theme	inner-sphere	1328:1339	arg1	complex					1349:1355	inner-sphere surface complex	1328:1355	inner-sphere surface complex	1328:1355	Adsorption mechanism showed that phosphate was adsorbed mainly through electrostatic force, ion-exchange, and inner-sphere surface complex.					
32580048	5	61	theme	optimum	1084:1090	arg1	range					1095:1099	The optimum pH range	1080:1099	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3	1080:1144	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3 was from 3.0 to 10.0, and in this range Fe release was less than 0.03%.					
32580048	3	62	theme	exposed	768:774	arg1	FeOHx					810:814	FeOHx	810:814	FeOHx	810:814	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	62	theme	exposed	768:774	arg1	Cl-					833:835	exchangeable Cl-	820:835	exchangeable Cl-	820:835	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	62	theme	exposed	768:774	arg1	sites					794:798	the more exposed adsorption active sites	759:798	the more exposed adsorption active sites including FeOHx and exchangeable Cl-	759:835	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	5	63	theme	Fe	1137:1138	arg1	/SCB3					1140:1144	MIL-101(Fe)/SCB3	1129:1144	MIL-101(Fe)/SCB3	1129:1144	The optimum pH range for phosphate adsorption on MIL-101(Fe)/SCB3 was from 3.0 to 10.0, and in this range Fe release was less than 0.03%.					
32580048	1	64	with	bagasse	147:153	arg1	selectivity					195:205	selectivity	195:205	selectivity toward phosphate	195:222	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	1	64	with	bagasse	147:153	arg1	capacity					182:189	high adsorption capacity	166:189	high adsorption capacity	166:189	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	2	65	from	behavior	382:389	arg1	composite					425:433	the composite	421:433	the composite prepared at the optimum bagasse size	421:470	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	4	66	theme	MIL-101	900:906	arg1	/SCB3					911:915	the as prepared MIL-101(Fe)/SCB3	884:915	the as prepared MIL-101(Fe)/SCB3	884:915	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	2	67	theme	adsorption	371:380	arg1	behavior					382:389	adsorption behavior	371:389	adsorption behavior	371:389	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32580048	3	68	theme	Fe	568:569	arg1	mesh					554:557	200-300 mesh	546:557	200-300 mesh (MIL-101(Fe)/SCB3)	546:576	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	3	68	theme	Fe	568:569	arg1	/SCB3					571:575	MIL-101(Fe)/SCB3	560:575	MIL-101(Fe)/SCB3	560:575	Results showed that composite prepared with bagasse size of 200-300 mesh (MIL-101(Fe)/SCB3) showed much higher adsorption capacity than SCB, blank MIL-101(Fe) and the composites prepared with the other bagasse size, which was due to the more positively charged surface and the more exposed adsorption active sites including FeOHx and exchangeable Cl-.					
32580048	1	69	theme	MIL-101	125:131	arg1	SCB					156:158	SCB	156:158	SCB	156:158	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	1	69	theme	MIL-101	125:131	arg1	bagasse					147:153	MIL-101(Fe)/sugarcane bagasse	125:153	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate	125:222	MIL-101(Fe)/sugarcane bagasse (SCB) with high adsorption capacity and selectivity toward phosphate was prepared through in-situ synthesis method.					
32580048	4	70	theme	cationic	982:989	arg1	ions					1003:1006	the common cationic and anionic ions	971:1006	the common cationic and anionic ions	971:1006	Co-ions experimental results illustrated that the as prepared MIL-101(Fe)/SCB3 showed high adsorption affinity toward phosphate, and the common cationic and anionic ions exhibited negligible effects on phosphate adsorption capacity and rate.					
32580048	7	71	theme	aqueous	1469:1475	arg1	solution					1477:1484	aqueous solution	1469:1484	aqueous solution	1469:1484	Simulated wastewater treatment experiment showed that MIL-101(Fe)/SCB3 could efficiently remove phosphate from aqueous solution.					
32580048	0	72	theme	MIL-101	56:62	arg1	composite					76:84	MIL-101(Fe)/bagasse composite	56:84	MIL-101(Fe)/bagasse composite	56:84	Selective removal of phosphate from aqueous solution by MIL-101(Fe)/bagasse composite prepared through bagasse size control.					
32580048	7	73	theme	treatment	1379:1387	arg1	experiment					1389:1398	Simulated wastewater treatment experiment	1358:1398	Simulated wastewater treatment experiment	1358:1398	Simulated wastewater treatment experiment showed that MIL-101(Fe)/SCB3 could efficiently remove phosphate from aqueous solution.					
32580048	2	74	from	mechanism	395:403	arg1	composite					425:433	the composite	421:433	the composite prepared at the optimum bagasse size	421:470	Effects of bagasse size on the morphology and performances of the composites were investigated, and adsorption behavior and mechanism of phosphate on the composite prepared at the optimum bagasse size were studied.					
32224182	7	0	theme	FT-IR	1076:1080	arg1	analysis					1082:1089	FT-IR analysis	1076:1089	FT-IR analysis	1076:1089	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	8	1	theme	strongest	1412:1420	arg1	activity					1434:1441	the strongest antioxidant activity	1408:1441	the strongest antioxidant activity	1408:1441	In addition, compared to total AAP, the antioxidant activities in vitro of D-AAP-VI were significantly improved (p < 0.05) and D-AAP-VI showed the strongest antioxidant activity.					
32224182	6	2	theme	degradation	937:947	arg1	products					949:956	The degradation products	933:956	The degradation products	933:956	The degradation products were classified to different molecular weight (Mw) polysaccharide fragments using membrane separation technology.					
32224182	7	3	dep	analysis	1082:1089	arg1	The					1072:1074	The	1072:1074	The	1072:1074	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	8	4	theme	D-AAP-VI	1340:1347	arg1	activities					1317:1326	the antioxidant activities	1301:1326	the antioxidant activities in vitro of D-AAP-VI	1301:1347	In addition, compared to total AAP, the antioxidant activities in vitro of D-AAP-VI were significantly improved (p < 0.05) and D-AAP-VI showed the strongest antioxidant activity.					
32224182	9	5	theme	suitable	1505:1512	arg1	way					1514:1516	a suitable way	1503:1516	a suitable way to improve the antioxidant activities of natural polysaccharides	1503:1581	These results indicated that biological degradation may be a suitable way to improve the antioxidant activities of natural polysaccharides.					
32224182	9	5	theme	suitable	1505:1512	arg1	degradation					1484:1494	biological degradation	1473:1494	biological degradation	1473:1494	These results indicated that biological degradation may be a suitable way to improve the antioxidant activities of natural polysaccharides.					
32224182	3	6	theme	independent	373:383	arg1	factors					385:391	4 independent factors	371:391	4 independent factors	371:391	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	3	7	dep	X4	492:493	arg1	d					500:500	4-6 d	496:500	X4: 4-6 d	492:500	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	3	8	dep	content	400:406	arg1	X1					409:410	X1	409:410	X1: 40-80%	409:418	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	6	9	theme	polysaccharide	1009:1022	arg1	fragments					1024:1032	different molecular weight (Mw) polysaccharide fragments	977:1032	different molecular weight (Mw) polysaccharide fragments using membrane separation technology	977:1069	The degradation products were classified to different molecular weight (Mw) polysaccharide fragments using membrane separation technology.					
32224182	6	10	theme	membrane	1040:1047	arg1	technology					1060:1069	membrane separation technology	1040:1069	membrane separation technology	1040:1069	The degradation products were classified to different molecular weight (Mw) polysaccharide fragments using membrane separation technology.					
32224182	2	11	used	used	310:313	arg2	RSM					301:303	RSM	301:303	RSM	301:303	Response surface methodology (RSM) was used to optimize the fermentation conditions.					
32224182	2	11	used	used	310:313	arg2	methodology					288:298	Response surface methodology	271:298	Response surface methodology (RSM)	271:304	Response surface methodology (RSM) was used to optimize the fermentation conditions.					
32224182	0	12	theme	Trichoderma	101:111	arg1	viride					113:118	Trichoderma viride	101:118	Trichoderma viride	101:118	Response surface methodology for the fermentation of polysaccharides from Auricularia auricula using Trichoderma viride and their antioxidant activities.					
32224182	1	13	used	used	182:185	arg2	technology					167:176	Fermentation technology	154:176	Fermentation technology	154:176	Fermentation technology was used to improve the antioxidant activities of Auricularia auricula polysaccharide (AAP).					
32224182	3	14	theme	time	486:489	arg1	effects					360:366	The effects	356:366	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency	356:542	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	3	15	dep	time	486:489	arg1	X4					492:493	X4	492:493	X4: 4-6 d	492:500	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	4	16	theme	RSM	564:566	arg1	results					568:574	The RSM results	560:574	The RSM results	560:574	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	3	17	dep	X1	409:410	arg1	%					418:418	40-80%	413:418	X1: 40-80%	409:418	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	5	18	theme	degradation	830:840	arg1	%					863:863	26.89 ± 0.14%	851:863	26.89 ± 0.14%	851:863	Under the optimal conditions, the degradation rate was 26.89 ± 0.14%, without significant differences with the predicted value (27.03%).					
32224182	5	18	theme	degradation	830:840	arg1	rate					842:845	the degradation rate	826:845	the degradation rate	826:845	Under the optimal conditions, the degradation rate was 26.89 ± 0.14%, without significant differences with the predicted value (27.03%).					
32224182	3	19	theme	inoculation	422:432	arg1	amount					434:439	inoculation amount	422:439	inoculation amount (X2: 2-20%)	422:451	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	8	20	dep	in	1328:1329	arg1	vitro					1331:1335	vitro	1331:1335	vitro	1331:1335	In addition, compared to total AAP, the antioxidant activities in vitro of D-AAP-VI were significantly improved (p < 0.05) and D-AAP-VI showed the strongest antioxidant activity.					
32224182	0	21	from	fermentation	37:48	arg1	auricula					86:93	Auricularia auricula	74:93	Auricularia auricula	74:93	Response surface methodology for the fermentation of polysaccharides from Auricularia auricula using Trichoderma viride and their antioxidant activities.					
32224182	3	22	dep	temperature	454:464	arg1	X3					467:468	X3	467:468	X3: 24-32 °C	467:478	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	3	23	theme	amount	434:439	arg1	effects					360:366	The effects	356:366	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency	356:542	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	0	24	theme	surface	9:15	arg1	methodology					17:27	Response surface methodology	0:27	Response surface methodology for the fermentation of polysaccharides from Auricularia auricula	0:93	Response surface methodology for the fermentation of polysaccharides from Auricularia auricula using Trichoderma viride and their antioxidant activities.					
32224182	4	25	theme	significant	705:715	arg1	differences					717:727	no significant differences	702:727	no significant differences between the practical and the predictive values for each response	702:793	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	7	26	theme	degraded	1134:1141	arg1	D-AAP-VI					1148:1155	D-AAP-VI	1148:1155	D-AAP-VI	1148:1155	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	7	26	theme	degraded	1134:1141	arg1	AAP					1143:1145	degraded AAP	1134:1145	degraded AAP (D-AAP-VI)	1134:1156	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	2	27	theme	fermentation	331:342	arg1	conditions					344:353	the fermentation conditions	327:353	the fermentation conditions	327:353	Response surface methodology (RSM) was used to optimize the fermentation conditions.					
32224182	1	28	theme	antioxidant	202:212	arg1	activities					214:223	the antioxidant activities	198:223	the antioxidant activities of Auricularia auricula polysaccharide (AAP)	198:268	Fermentation technology was used to improve the antioxidant activities of Auricularia auricula polysaccharide (AAP).					
32224182	0	29	theme	Response	0:7	arg1	methodology					17:27	Response surface methodology	0:27	Response surface methodology for the fermentation of polysaccharides from Auricularia auricula	0:93	Response surface methodology for the fermentation of polysaccharides from Auricularia auricula using Trichoderma viride and their antioxidant activities.					
32224182	8	30	theme	antioxidant	1305:1315	arg1	activities					1317:1326	the antioxidant activities	1301:1326	the antioxidant activities in vitro of D-AAP-VI	1301:1347	In addition, compared to total AAP, the antioxidant activities in vitro of D-AAP-VI were significantly improved (p < 0.05) and D-AAP-VI showed the strongest antioxidant activity.					
32224182	3	31	dep	X3	467:468	arg1	24-32 °C					471:478	24-32 °C	471:478	X3: 24-32 °C	467:478	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	7	32	theme	furan	1185:1189	arg1	D-AAP-VI					1170:1177	D-AAP-VI	1170:1177	D-AAP-VI	1170:1177	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	7	32	theme	furan	1185:1189	arg1	different					1222:1230	different	1222:1230	different	1222:1230	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	7	32	theme	furan	1185:1189	arg1	polysaccharide					1196:1209	a furan type polysaccharide	1183:1209	a furan type polysaccharide	1183:1209	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	3	33	theme	biological	510:519	arg1	efficiency					533:542	the biological degradation efficiency	506:542	the biological degradation efficiency	506:542	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	9	34	theme	antioxidant	1533:1543	arg1	activities					1545:1554	the antioxidant activities	1529:1554	the antioxidant activities of natural polysaccharides	1529:1581	These results indicated that biological degradation may be a suitable way to improve the antioxidant activities of natural polysaccharides.					
32224182	4	35	dep	X1	630:631	arg1	%					649:649	12.4%	645:649	12.4%	645:649	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	4	35	dep	X1	630:631	arg1	%					638:638	61.7%	634:638	61.7%	634:638	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	4	35	dep	X1	630:631	arg1	X4					665:666	X4	665:666	X4	665:666	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	4	35	dep	X1	630:631	arg1	5.5					669:671	5.5	669:671	5.5	669:671	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	7	36	from	AAP	1247:1249	arg1	D-AAP-VI					1170:1177	D-AAP-VI	1170:1177	D-AAP-VI	1170:1177	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	7	36	from	AAP	1247:1249	arg1	different					1222:1230	different	1222:1230	different	1222:1230	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	7	36	from	AAP	1247:1249	arg1	polysaccharide					1196:1209	a furan type polysaccharide	1183:1209	a furan type polysaccharide	1183:1209	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	3	37	theme	water	394:398	arg1	content					400:406	water content	394:406	water content (X1: 40-80%)	394:419	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	0	38	theme	antioxidant	130:140	arg1	activities					142:151	their antioxidant activities	124:151	their antioxidant activities	124:151	Response surface methodology for the fermentation of polysaccharides from Auricularia auricula using Trichoderma viride and their antioxidant activities.					
32224182	6	39	theme	weight	997:1002	arg1	fragments					1024:1032	different molecular weight (Mw) polysaccharide fragments	977:1032	different molecular weight (Mw) polysaccharide fragments using membrane separation technology	977:1069	The degradation products were classified to different molecular weight (Mw) polysaccharide fragments using membrane separation technology.					
32224182	1	40	theme	Auricularia	228:238	arg1	AAP					265:267	AAP	265:267	AAP	265:267	Fermentation technology was used to improve the antioxidant activities of Auricularia auricula polysaccharide (AAP).					
32224182	1	40	theme	Auricularia	228:238	arg1	polysaccharide					249:262	Auricularia auricula polysaccharide	228:262	Auricularia auricula polysaccharide (AAP)	228:268	Fermentation technology was used to improve the antioxidant activities of Auricularia auricula polysaccharide (AAP).					
32224182	6	41	theme	different	977:985	arg1	fragments					1024:1032	different molecular weight (Mw) polysaccharide fragments	977:1032	different molecular weight (Mw) polysaccharide fragments using membrane separation technology	977:1069	The degradation products were classified to different molecular weight (Mw) polysaccharide fragments using membrane separation technology.					
32224182	0	42	from	auricula	86:93	arg1	fermentation					37:48	the fermentation	33:48	the fermentation of polysaccharides from Auricularia auricula	33:93	Response surface methodology for the fermentation of polysaccharides from Auricularia auricula using Trichoderma viride and their antioxidant activities.					
32224182	0	42	from	auricula	86:93	arg1	polysaccharides					53:67	polysaccharides	53:67	polysaccharides from Auricularia auricula	53:93	Response surface methodology for the fermentation of polysaccharides from Auricularia auricula using Trichoderma viride and their antioxidant activities.					
32224182	7	43	theme	total	1241:1245	arg1	type					1258:1261	pyran type	1252:1261	pyran type	1252:1261	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	7	43	theme	total	1241:1245	arg1	AAP					1247:1249	the total AAP	1237:1249	the total AAP (pyran type)	1237:1262	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	6	44	theme	separation	1049:1058	arg1	technology					1060:1069	membrane separation technology	1040:1069	membrane separation technology	1040:1069	The degradation products were classified to different molecular weight (Mw) polysaccharide fragments using membrane separation technology.					
32224182	1	45	theme	auricula	240:247	arg1	AAP					265:267	AAP	265:267	AAP	265:267	Fermentation technology was used to improve the antioxidant activities of Auricularia auricula polysaccharide (AAP).					
32224182	1	45	theme	auricula	240:247	arg1	polysaccharide					249:262	Auricularia auricula polysaccharide	228:262	Auricularia auricula polysaccharide (AAP)	228:268	Fermentation technology was used to improve the antioxidant activities of Auricularia auricula polysaccharide (AAP).					
32224182	7	46	theme	type	1191:1194	arg1	D-AAP-VI					1170:1177	D-AAP-VI	1170:1177	D-AAP-VI	1170:1177	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	7	46	theme	type	1191:1194	arg1	different					1222:1230	different	1222:1230	different	1222:1230	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	7	46	theme	type	1191:1194	arg1	polysaccharide					1196:1209	a furan type polysaccharide	1183:1209	a furan type polysaccharide	1183:1209	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	9	47	theme	natural	1559:1565	arg1	polysaccharides					1567:1581	natural polysaccharides	1559:1581	natural polysaccharides	1559:1581	These results indicated that biological degradation may be a suitable way to improve the antioxidant activities of natural polysaccharides.					
32224182	5	48	theme	significant	874:884	arg1	differences					886:896	significant differences	874:896	significant differences with the predicted value (27.03%)	874:930	Under the optimal conditions, the degradation rate was 26.89 ± 0.14%, without significant differences with the predicted value (27.03%).					
32224182	9	49	theme	biological	1473:1482	arg1	way					1514:1516	a suitable way	1503:1516	a suitable way to improve the antioxidant activities of natural polysaccharides	1503:1581	These results indicated that biological degradation may be a suitable way to improve the antioxidant activities of natural polysaccharides.					
32224182	9	49	theme	biological	1473:1482	arg1	degradation					1484:1494	biological degradation	1473:1494	biological degradation	1473:1494	These results indicated that biological degradation may be a suitable way to improve the antioxidant activities of natural polysaccharides.					
32224182	1	50	theme	polysaccharide	249:262	arg1	activities					214:223	the antioxidant activities	198:223	the antioxidant activities of Auricularia auricula polysaccharide (AAP)	198:268	Fermentation technology was used to improve the antioxidant activities of Auricularia auricula polysaccharide (AAP).					
32224182	0	51	theme	polysaccharides	53:67	arg1	fermentation					37:48	the fermentation	33:48	the fermentation of polysaccharides from Auricularia auricula	33:93	Response surface methodology for the fermentation of polysaccharides from Auricularia auricula using Trichoderma viride and their antioxidant activities.					
32224182	5	52	with	differences	886:896	arg1	value					917:921	the predicted value	903:921	the predicted value (27.03%)	903:930	Under the optimal conditions, the degradation rate was 26.89 ± 0.14%, without significant differences with the predicted value (27.03%).					
32224182	5	52	with	differences	886:896	arg1	%					929:929	27.03%	924:929	27.03%	924:929	Under the optimal conditions, the degradation rate was 26.89 ± 0.14%, without significant differences with the predicted value (27.03%).					
32224182	9	53	theme	polysaccharides	1567:1581	arg1	activities					1545:1554	the antioxidant activities	1529:1554	the antioxidant activities of natural polysaccharides	1529:1581	These results indicated that biological degradation may be a suitable way to improve the antioxidant activities of natural polysaccharides.					
32224182	8	54	theme	total	1290:1294	arg1	AAP					1296:1298	total AAP	1290:1298	total AAP	1290:1298	In addition, compared to total AAP, the antioxidant activities in vitro of D-AAP-VI were significantly improved (p < 0.05) and D-AAP-VI showed the strongest antioxidant activity.					
32224182	4	55	theme	Verification	676:687	arg1	tests					689:693	X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests	630:693	X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests	630:693	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	4	56	theme	X1	630:631	arg1	tests					689:693	X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests	630:693	X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests	630:693	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	8	57	theme	antioxidant	1422:1432	arg1	activity					1434:1441	the strongest antioxidant activity	1408:1441	the strongest antioxidant activity	1408:1441	In addition, compared to total AAP, the antioxidant activities in vitro of D-AAP-VI were significantly improved (p < 0.05) and D-AAP-VI showed the strongest antioxidant activity.					
32224182	3	58	dep	amount	434:439	arg1	X2					442:443	X2	442:443	X2: 2-20%	442:450	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	3	59	theme	temperature	454:464	arg1	effects					360:366	The effects	356:366	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency	356:542	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	2	60	theme	Response	271:278	arg1	RSM					301:303	RSM	301:303	RSM	301:303	Response surface methodology (RSM) was used to optimize the fermentation conditions.					
32224182	2	60	theme	Response	271:278	arg1	methodology					288:298	Response surface methodology	271:298	Response surface methodology (RSM)	271:304	Response surface methodology (RSM) was used to optimize the fermentation conditions.					
32224182	5	61	theme	predicted	907:915	arg1	value					917:921	the predicted value	903:921	the predicted value (27.03%)	903:930	Under the optimal conditions, the degradation rate was 26.89 ± 0.14%, without significant differences with the predicted value (27.03%).					
32224182	5	61	theme	predicted	907:915	arg1	%					929:929	27.03%	924:929	27.03%	924:929	Under the optimal conditions, the degradation rate was 26.89 ± 0.14%, without significant differences with the predicted value (27.03%).					
32224182	4	62	theme	optimal	592:598	arg1	conditions					613:622	the optimal fermentation conditions	588:622	the optimal fermentation conditions	588:622	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	7	63	theme	AAP	1143:1145	arg1	analysis					1082:1089	FT-IR analysis	1076:1089	FT-IR analysis	1076:1089	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	7	63	theme	AAP	1143:1145	arg1	analysis					1122:1129	monosaccharide composition analysis	1095:1129	monosaccharide composition analysis	1095:1129	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	5	64	theme	optimal	806:812	arg1	conditions					814:823	the optimal conditions	802:823	the optimal conditions	802:823	Under the optimal conditions, the degradation rate was 26.89 ± 0.14%, without significant differences with the predicted value (27.03%).					
32224182	7	65	theme	pyran	1252:1256	arg1	type					1258:1261	pyran type	1252:1261	pyran type	1252:1261	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	7	65	theme	pyran	1252:1256	arg1	AAP					1247:1249	the total AAP	1237:1249	the total AAP (pyran type)	1237:1262	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	4	66	theme	predictive	759:768	arg1	values					770:775	the predictive values	755:775	the predictive values for each response	755:793	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	3	67	dep	X2	442:443	arg1	%					450:450	2-20%	446:450	X2: 2-20%	442:450	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	8	68	theme	in	1328:1329	arg1	activities					1317:1326	the antioxidant activities	1301:1326	the antioxidant activities in vitro of D-AAP-VI	1301:1347	In addition, compared to total AAP, the antioxidant activities in vitro of D-AAP-VI were significantly improved (p < 0.05) and D-AAP-VI showed the strongest antioxidant activity.					
32224182	1	69	theme	Fermentation	154:165	arg1	technology					167:176	Fermentation technology	154:176	Fermentation technology	154:176	Fermentation technology was used to improve the antioxidant activities of Auricularia auricula polysaccharide (AAP).					
32224182	6	70	theme	molecular	987:995	arg1	Mw					1005:1006	Mw	1005:1006	Mw	1005:1006	The degradation products were classified to different molecular weight (Mw) polysaccharide fragments using membrane separation technology.					
32224182	6	70	theme	molecular	987:995	arg1	weight					997:1002	molecular weight	987:1002	different molecular weight (Mw) polysaccharide fragments using membrane separation technology	977:1069	The degradation products were classified to different molecular weight (Mw) polysaccharide fragments using membrane separation technology.					
32224182	8	71	dep	improved	1368:1375	arg1	p < 0.05					1378:1385	p < 0.05	1378:1385	p < 0.05	1378:1385	In addition, compared to total AAP, the antioxidant activities in vitro of D-AAP-VI were significantly improved (p < 0.05) and D-AAP-VI showed the strongest antioxidant activity.					
32224182	3	72	from	effects	360:366	arg1	efficiency					533:542	the biological degradation efficiency	506:542	the biological degradation efficiency	506:542	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	2	73	theme	surface	280:286	arg1	RSM					301:303	RSM	301:303	RSM	301:303	Response surface methodology (RSM) was used to optimize the fermentation conditions.					
32224182	2	73	theme	surface	280:286	arg1	methodology					288:298	Response surface methodology	271:298	Response surface methodology (RSM)	271:304	Response surface methodology (RSM) was used to optimize the fermentation conditions.					
32224182	4	74	theme	fermentation	600:611	arg1	conditions					613:622	the optimal fermentation conditions	588:622	the optimal fermentation conditions	588:622	The RSM results showed that the optimal fermentation conditions were: X1: 61.7%, X2: 12.4%, X3: 31.0 °C, X4: 5.5 d. Verification tests showed no significant differences between the practical and the predictive values for each response.					
32224182	7	75	theme	monosaccharide	1095:1108	arg1	analysis					1122:1129	monosaccharide composition analysis	1095:1129	monosaccharide composition analysis	1095:1129	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	3	76	theme	degradation	521:531	arg1	efficiency					533:542	the biological degradation efficiency	506:542	the biological degradation efficiency	506:542	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	3	77	theme	content	400:406	arg1	effects					360:366	The effects	356:366	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency	356:542	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
32224182	7	78	theme	composition	1110:1120	arg1	analysis					1122:1129	monosaccharide composition analysis	1095:1129	monosaccharide composition analysis	1095:1129	The FT-IR analysis and monosaccharide composition analysis of degraded AAP (D-AAP-VI) showed that D-AAP-VI was a furan type polysaccharide, which was different from the total AAP (pyran type).					
32224182	3	79	theme	factors	385:391	arg1	effects					360:366	The effects	356:366	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency	356:542	The effects of 4 independent factors: water content (X1: 40-80%), inoculation amount (X2: 2-20%), temperature (X3: 24-32 °C), and time (X4: 4-6 d) on the biological degradation efficiency were evaluated.					
33254961	0	0	theme	chitosan/oxidized	74:90	arg1	sulfate					104:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	0	0	theme	chitosan/oxidized	74:90	arg1	N					56:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	8	1	theme	hemostatic	1514:1523	arg1	hydrogel					1525:1532	an injectable, self-healing, antibacterial, and hemostatic hydrogel	1466:1532	an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides	1466:1561	Our work describing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides will likely hold good potential in serving as an enabling wound dressing material.					
33254961	7	2	theme	excellent	1404:1412	arg1	performance					1433:1443	excellent in vivo hemostatic performance	1404:1443	excellent in vivo hemostatic performance	1404:1443	Importantly, the hydrogel tightly adhered to the biological tissue and demonstrated excellent in vivo hemostatic performance.					
33254961	8	3	theme	self-healing	1481:1492	arg1	hydrogel					1525:1532	an injectable, self-healing, antibacterial, and hemostatic hydrogel	1466:1532	an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides	1466:1561	Our work describing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides will likely hold good potential in serving as an enabling wound dressing material.					
33254961	1	4	theme	injectable	169:178	arg1	hydrogels					180:188	Biodegradable and injectable hydrogels	151:188	Biodegradable and injectable hydrogels derived from natural polysaccharides	151:225	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	4	5	dep	hydrogel	949:956	arg1	O-CMC2/OCS1					937:947	the N,O-CMC2/OCS1 hydrogel	931:956	O-CMC2/OCS1	937:947	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	0	6	theme	O-carboxymethyl	58:72	arg1	sulfate					104:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	0	6	theme	O-carboxymethyl	58:72	arg1	N					56:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	2	7	attach	derived	514:520	arg2	hydrogel					505:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	2	7	attach	derived	514:520	arg1	hybrid					531:536	the hybrid	527:536	the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS)	527:615	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	5	8	theme	NIH/3T3	1042:1048	arg1	cells					1050:1054	NIH/3T3 cells	1042:1054	NIH/3T3 cells	1042:1054	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	3	9	theme	N	860:860	arg1	groups					850:855	the -NH- functional groups	830:855	the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS	830:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	9	theme	N	860:860	arg1	OCS					902:904	OCS	902:904	OCS	902:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	9	theme	N	860:860	arg1	groups					892:897	the -CHO functional groups	872:897	the -CHO functional groups of OCS	872:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	9	theme	N	860:860	arg1	O-CMC					862:866	O-CMC	862:866	O-CMC	862:866	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	9	theme	N	860:860	arg1	N					860:860	N	860:860	N	860:860	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	5	10	theme	cells	1050:1054	arg1	viability					1029:1037	The viability	1025:1037	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract	1025:1125	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	5	10	theme	cells	1050:1054	arg1	%					1141:1141	roughly 85%	1131:1141	roughly 85%	1131:1141	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	5	11	theme	N	1096:1096	arg1	extract					1119:1125	the N,O-CMC2/OCS1 hydrogel extract	1092:1125	extract	1119:1125	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	8	12	theme	dressing	1627:1634	arg1	material					1636:1643	an enabling wound dressing material	1609:1643	an enabling wound dressing material	1609:1643	Our work describing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides will likely hold good potential in serving as an enabling wound dressing material.					
33254961	4	13	contain	had	958:960	arg2	performances					1011:1022	stable performances	1004:1022	stable performances	1004:1022	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	4	13	contain	had	958:960	arg2	time					987:990	relatively long gelation time	962:990	relatively long gelation time (133 s)	962:998	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	4	13	contain	had	958:960	arg2	133 s					993:997	133 s	993:997	133 s	993:997	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	4	13	contain	had	958:960	arg1	hydrogel					949:956	the N,O-CMC2/OCS1 hydrogel	931:956	hydrogel	949:956	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	7	14	theme	biological	1369:1378	arg1	tissue					1380:1385	the biological tissue	1365:1385	the biological tissue	1365:1385	Importantly, the hydrogel tightly adhered to the biological tissue and demonstrated excellent in vivo hemostatic performance.					
33254961	1	15	theme	minimal	302:308	arg1	invasiveness					310:321	their minimal invasiveness	296:321	their minimal invasiveness	296:321	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	0	16	theme	chondroitin	92:102	arg1	sulfate					104:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	0	16	theme	chondroitin	92:102	arg1	N					56:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	3	17	theme	physiological	724:736	arg1	conditions					738:747	physiological conditions	724:747	physiological conditions	724:747	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	2	18	theme	chemical	644:651	arg1	crosslinking					653:664	any chemical crosslinking	640:664	any chemical crosslinking	640:664	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	3	19	theme	functional	881:890	arg1	OCS					902:904	OCS	902:904	OCS	902:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	19	theme	functional	881:890	arg1	groups					892:897	the -CHO functional groups	872:897	the -CHO functional groups of OCS	872:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	6	20	theme	antibacterial	1286:1298	arg1	ability					1300:1306	the inherent antibacterial ability	1273:1306	the inherent antibacterial ability of N,O-CMC	1273:1317	Besides, the N,O-CMC2/OCS1 hydrogel revealed excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC.					
33254961	2	21	theme	O-carboxymethyl	543:557	arg1	N					569:569	N	569:569	N	569:569	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	2	21	theme	O-carboxymethyl	543:557	arg1	chitosan					559:566	O-carboxymethyl chitosan	543:566	O-carboxymethyl chitosan (N,O-CMC)	543:576	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	5	22	dep	extract	1119:1125	arg1	O-CMC2/OCS1					1098:1108	the N,O-CMC2/OCS1 hydrogel extract	1092:1125	O-CMC2/OCS1	1098:1108	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	6	23	theme	N	1199:1199	arg1	hydrogel					1213:1220	the N,O-CMC2/OCS1 hydrogel	1195:1220	hydrogel	1213:1220	Besides, the N,O-CMC2/OCS1 hydrogel revealed excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC.					
33254961	3	24	theme	groups	892:897	arg1	groups					850:855	the -NH- functional groups	830:855	the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS	830:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	24	theme	groups	892:897	arg1	OCS					902:904	OCS	902:904	OCS	902:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	24	theme	groups	892:897	arg1	groups					892:897	the -CHO functional groups	872:897	the -CHO functional groups of OCS	872:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	24	theme	groups	892:897	arg1	O-CMC					862:866	O-CMC	862:866	O-CMC	862:866	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	24	theme	groups	892:897	arg1	N					860:860	N	860:860	N	860:860	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	2	25	theme	in-situ-injectable	426:443	arg1	hydrogel					505:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	4	26	theme	long	973:976	arg1	133 s					993:997	133 s	993:997	133 s	993:997	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	4	26	theme	long	973:976	arg1	time					987:990	relatively long gelation time	962:990	relatively long gelation time (133 s)	962:998	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	2	27	theme	self-healing	446:457	arg1	hydrogel					505:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	8	28	attach	derived	1534:1540	arg2	hydrogel					1525:1532	an injectable, self-healing, antibacterial, and hemostatic hydrogel	1466:1532	an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides	1466:1561	Our work describing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides will likely hold good potential in serving as an enabling wound dressing material.					
33254961	8	28	attach	derived	1534:1540	arg1	polysaccharides					1547:1561	polysaccharides	1547:1561	polysaccharides	1547:1561	Our work describing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides will likely hold good potential in serving as an enabling wound dressing material.					
33254961	3	29	theme	Schiff	801:806	arg1	base					808:811	the Schiff base	797:811	the Schiff base	797:811	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	5	30	theme	hydrogel	1110:1117	arg1	extract					1119:1125	the N,O-CMC2/OCS1 hydrogel extract	1092:1125	extract	1119:1125	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	5	31	theme	cells	1072:1076	arg1	viability					1029:1037	The viability	1025:1037	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract	1025:1125	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	5	31	theme	cells	1072:1076	arg1	%					1141:1141	roughly 85%	1131:1141	roughly 85%	1131:1141	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	7	32	theme	in	1414:1415	arg1	performance					1433:1443	excellent in vivo hemostatic performance	1404:1443	excellent in vivo hemostatic performance	1404:1443	Importantly, the hydrogel tightly adhered to the biological tissue and demonstrated excellent in vivo hemostatic performance.					
33254961	1	33	theme	natural	203:209	arg1	polysaccharides					211:225	natural polysaccharides	203:225	natural polysaccharides	203:225	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	0	34	theme	Injectable	0:9	arg1	sulfate					104:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	0	34	theme	Injectable	0:9	arg1	N					56:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	3	35	dep	hydrogel	683:690	arg1	O-CMC/OCS					673:681	The N,O-CMC/OCS hydrogel	667:690	O-CMC/OCS	673:681	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	4	36	theme	gelation	978:985	arg1	133 s					993:997	133 s	993:997	133 s	993:997	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	4	36	theme	gelation	978:985	arg1	time					987:990	relatively long gelation time	962:990	relatively long gelation time (133 s)	962:998	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	2	37	theme	hemostatic	475:484	arg1	hydrogel					505:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	3	38	theme	N	764:764	arg1	ratio					779:783	the N,O-CMC-to-OCS ratio	760:783	ratio	779:783	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	0	39	theme	wound	135:139	arg1	dressing					141:148	wound dressing	135:148	wound dressing	135:148	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	8	40	theme	antibacterial	1495:1507	arg1	hydrogel					1525:1532	an injectable, self-healing, antibacterial, and hemostatic hydrogel	1466:1532	an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides	1466:1561	Our work describing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides will likely hold good potential in serving as an enabling wound dressing material.					
33254961	2	41	theme	antibacterial	460:472	arg1	hydrogel					505:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	0	42	theme	self-healing	12:23	arg1	sulfate					104:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	0	42	theme	self-healing	12:23	arg1	N					56:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	8	43	theme	injectable	1469:1478	arg1	hydrogel					1525:1532	an injectable, self-healing, antibacterial, and hemostatic hydrogel	1466:1532	an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides	1466:1561	Our work describing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides will likely hold good potential in serving as an enabling wound dressing material.					
33254961	6	44	theme	inherent	1277:1284	arg1	ability					1300:1306	the inherent antibacterial ability	1273:1306	the inherent antibacterial ability of N,O-CMC	1273:1317	Besides, the N,O-CMC2/OCS1 hydrogel revealed excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC.					
33254961	5	45	theme	endothelial	1060:1070	arg1	cells					1072:1076	endothelial cells	1060:1076	endothelial cells	1060:1076	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	6	46	theme	N	1311:1311	arg1	ability					1300:1306	the inherent antibacterial ability	1273:1306	the inherent antibacterial ability of N,O-CMC	1273:1317	Besides, the N,O-CMC2/OCS1 hydrogel revealed excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC.					
33254961	7	47	dep	in	1414:1415	arg1	vivo					1417:1420	vivo	1417:1420	vivo	1417:1420	Importantly, the hydrogel tightly adhered to the biological tissue and demonstrated excellent in vivo hemostatic performance.					
33254961	1	48	attach	derived	190:196	arg2	hydrogels					180:188	Biodegradable and injectable hydrogels	151:188	Biodegradable and injectable hydrogels derived from natural polysaccharides	151:225	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	1	48	attach	derived	190:196	arg1	polysaccharides					211:225	natural polysaccharides	203:225	natural polysaccharides	203:225	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	3	49	theme	OCS	902:904	arg1	OCS					902:904	OCS	902:904	OCS	902:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	49	theme	OCS	902:904	arg1	groups					892:897	the -CHO functional groups	872:897	the -CHO functional groups of OCS	872:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	49	theme	OCS	902:904	arg1	O-CMC					862:866	O-CMC	862:866	O-CMC	862:866	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	49	theme	OCS	902:904	arg1	N					860:860	N	860:860	N	860:860	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	1	50	theme	extensive	242:250	arg1	attention					252:260	extensive attention	242:260	extensive attention in biomedical applications due to their minimal invasiveness and ability	242:333	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	0	51	theme	antibacterial	26:38	arg1	sulfate					104:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	0	51	theme	antibacterial	26:38	arg1	N					56:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	8	52	theme	good	1580:1583	arg1	potential					1585:1593	good potential	1580:1593	good potential	1580:1593	Our work describing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides will likely hold good potential in serving as an enabling wound dressing material.					
33254961	6	53	dep	hydrogel	1213:1220	arg1	O-CMC2/OCS1					1201:1211	the N,O-CMC2/OCS1 hydrogel	1195:1220	O-CMC2/OCS1	1201:1211	Besides, the N,O-CMC2/OCS1 hydrogel revealed excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC.					
33254961	2	54	theme	hydrogel	505:512	arg1	development					408:418	the development	404:418	the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking	404:664	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	1	55	theme	irregular	354:362	arg1	surfaces					370:377	the irregular wound surfaces	350:377	the irregular wound surfaces	350:377	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	2	56	theme	sulfate	603:609	arg1	hybrid					531:536	the hybrid	527:536	the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS)	527:615	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	4	57	theme	N	935:935	arg1	hydrogel					949:956	the N,O-CMC2/OCS1 hydrogel	931:956	hydrogel	949:956	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	1	58	from	attention	252:260	arg1	applications					276:287	biomedical applications	265:287	biomedical applications	265:287	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	2	59	theme	biocompatible	491:503	arg1	hydrogel					505:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel	423:512	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	1	60	theme	wound	364:368	arg1	surfaces					370:377	the irregular wound surfaces	350:377	the irregular wound surfaces	350:377	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	2	61	theme	chondroitin	591:601	arg1	OCS					612:614	OCS	612:614	OCS	612:614	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	2	61	theme	chondroitin	591:601	arg1	sulfate					603:609	oxidized chondroitin sulfate	582:609	oxidized chondroitin sulfate (OCS)	582:615	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	8	62	theme	enabling	1612:1619	arg1	material					1636:1643	an enabling wound dressing material	1609:1643	an enabling wound dressing material	1609:1643	Our work describing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides will likely hold good potential in serving as an enabling wound dressing material.					
33254961	3	63	dep	ratio	779:783	arg1	O-CMC-to-OCS					766:777	the N,O-CMC-to-OCS ratio	760:783	O-CMC-to-OCS	766:777	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	2	64	theme	oxidized	582:589	arg1	OCS					612:614	OCS	612:614	OCS	612:614	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	2	64	theme	oxidized	582:589	arg1	sulfate					603:609	oxidized chondroitin sulfate	582:609	oxidized chondroitin sulfate (OCS)	582:615	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	8	65	theme	wound	1621:1625	arg1	material					1636:1643	an enabling wound dressing material	1609:1643	an enabling wound dressing material	1609:1643	Our work describing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides will likely hold good potential in serving as an enabling wound dressing material.					
33254961	7	66	theme	hemostatic	1422:1431	arg1	performance					1433:1443	excellent in vivo hemostatic performance	1404:1443	excellent in vivo hemostatic performance	1404:1443	Importantly, the hydrogel tightly adhered to the biological tissue and demonstrated excellent in vivo hemostatic performance.					
33254961	6	67	theme	antibacterial	1241:1253	arg1	properties					1255:1264	excellent antibacterial properties	1231:1264	excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC	1231:1317	Besides, the N,O-CMC2/OCS1 hydrogel revealed excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC.					
33254961	5	68	theme	low	1167:1169	arg1	toxicity					1176:1183	its low cell toxicity	1163:1183	its low cell toxicity	1163:1183	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	6	69	theme	excellent	1231:1239	arg1	properties					1255:1264	excellent antibacterial properties	1231:1264	excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC	1231:1317	Besides, the N,O-CMC2/OCS1 hydrogel revealed excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC.					
33254961	3	70	theme	-NH-	834:837	arg1	groups					850:855	the -NH- functional groups	830:855	the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS	830:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	70	theme	-NH-	834:837	arg1	OCS					902:904	OCS	902:904	OCS	902:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	70	theme	-NH-	834:837	arg1	groups					892:897	the -CHO functional groups	872:897	the -CHO functional groups of OCS	872:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	70	theme	-NH-	834:837	arg1	O-CMC					862:866	O-CMC	862:866	O-CMC	862:866	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	70	theme	-NH-	834:837	arg1	N					860:860	N	860:860	N	860:860	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	1	71	theme	biomedical	265:274	arg1	applications					276:287	biomedical applications	265:287	biomedical applications	265:287	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	2	72	theme	N	541:541	arg1	hybrid					531:536	the hybrid	527:536	the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS)	527:615	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	3	73	theme	N	671:671	arg1	hydrogel					683:690	The N,O-CMC/OCS hydrogel	667:690	hydrogel	683:690	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	2	74	dep	N	569:569	arg1	O-CMC					571:575	O-CMC	571:575	O-CMC	571:575	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33254961	4	75	theme	stable	1004:1009	arg1	performances					1011:1022	stable performances	1004:1022	stable performances	1004:1022	The results showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable performances.					
33254961	1	76	theme	Biodegradable	151:163	arg1	hydrogels					180:188	Biodegradable and injectable hydrogels	151:188	Biodegradable and injectable hydrogels derived from natural polysaccharides	151:225	Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attention in biomedical applications due to their minimal invasiveness and ability to accommodate the irregular wound surfaces.					
33254961	3	77	theme	functional	839:848	arg1	groups					850:855	the -NH- functional groups	830:855	the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS	830:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	77	theme	functional	839:848	arg1	OCS					902:904	OCS	902:904	OCS	902:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	77	theme	functional	839:848	arg1	groups					892:897	the -CHO functional groups	872:897	the -CHO functional groups of OCS	872:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	77	theme	functional	839:848	arg1	O-CMC					862:866	O-CMC	862:866	O-CMC	862:866	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	77	theme	functional	839:848	arg1	N					860:860	N	860:860	N	860:860	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	6	78	theme	due	1266:1268	arg1	properties					1255:1264	excellent antibacterial properties	1231:1264	excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC	1231:1317	Besides, the N,O-CMC2/OCS1 hydrogel revealed excellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC.					
33254961	0	79	theme	hemostatic	45:54	arg1	sulfate					104:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	O-carboxymethyl chitosan/oxidized chondroitin sulfate	58:110	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	0	79	theme	hemostatic	45:54	arg1	N					56:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N	0:56	Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing.					
33254961	5	80	theme	cell	1171:1174	arg1	toxicity					1176:1183	its low cell toxicity	1163:1183	its low cell toxicity	1163:1183	The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extract was roughly 85%, which demonstrated its low cell toxicity.					
33254961	3	81	theme	O-CMC	862:866	arg1	groups					850:855	the -NH- functional groups	830:855	the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS	830:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	81	theme	O-CMC	862:866	arg1	OCS					902:904	OCS	902:904	OCS	902:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	81	theme	O-CMC	862:866	arg1	groups					892:897	the -CHO functional groups	872:897	the -CHO functional groups of OCS	872:904	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	81	theme	O-CMC	862:866	arg1	O-CMC					862:866	O-CMC	862:866	O-CMC	862:866	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	3	81	theme	O-CMC	862:866	arg1	N					860:860	N	860:860	N	860:860	The N,O-CMC/OCS hydrogel could be readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiff base reaction between the -NH- functional groups of N,O-CMC and the -CHO functional groups of OCS.					
33254961	2	82	theme	chitosan	559:566	arg1	hybrid					531:536	the hybrid	527:536	the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS)	527:615	In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic, and biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidized chondroitin sulfate (OCS), which did not require any chemical crosslinking.					
33667576	9	0	theme	fondaparinux	1205:1216	arg1	favour					1195:1200	favour	1195:1200	favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89)	1195:1350	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	4	1	from	benefit	436:442	arg1	management					498:507	the contemporary management	481:507	the contemporary management of NSTE-ACS	481:519	We aimed to assess the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS.					
33667576	11	2	theme	similar	1693:1699	arg1	results					1701:1707	similar results	1693:1707	similar results	1693:1707	Propensity-score-matching analysis yielded similar results.					
33667576	9	3	theme	enoxaparin	1225:1234	arg1	favour					1195:1200	favour	1195:1200	favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89)	1195:1350	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	4	4	theme	clinical	427:434	arg1	benefit					436:442	the net clinical benefit	419:442	the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS	419:519	We aimed to assess the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS.					
33667576	6	5	theme	cardiovascular	860:873	arg1	events					875:880	major adverse cardiovascular events	846:880	major adverse cardiovascular events at 30 days	846:891	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	5	6	theme	prospective	542:552	arg1	data					575:578	prospective multicenter registry data	542:578	prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017	542:681	METHODS Analysis of prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017.					
33667576	9	7	from	benefit	1184:1190	arg1	favour					1195:1200	favour	1195:1200	favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89)	1195:1350	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	9	8	theme	[SHR	1253:1256	arg1	enoxaparin					1225:1234	enoxaparin	1225:1234	enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92)	1225:1280	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	9	8	theme	[SHR	1253:1256	arg1	CI					1268:1269	Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92	1237:1279	Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92	1237:1279	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	9	9	from	reduction	1302:1310	arg1	bleeding					1315:1322	bleeding	1315:1322	bleeding	1315:1322	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	8	10	theme	diabetes	1040:1047	arg1	use					1083:1085	greater use	1075:1085	greater use of transradial approach in the fondaparinux group	1075:1135	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	8	10	theme	diabetes	1040:1047	arg1	prevalence					1026:1035	a lower prevalence	1018:1035	a lower prevalence of diabetes and renal impairment	1018:1068	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	12	11	theme	favorable	1794:1802	arg1	benefit					1817:1823	a favorable net clinical benefit	1792:1823	a favorable net clinical benefit	1792:1823	CONCLUSIONS In contemporary management of NSTE-ACS, fondaparinux seems to provide a favorable net clinical benefit compared with enoxaparin, primarily driven by a bleeding reduction.					
33667576	2	12	theme	non-ST-elevation	257:272	arg1	syndrome					289:296	non-ST-elevation acute coronary syndrome	257:296	non-ST-elevation acute coronary syndrome (NSTE-ACS)	257:307	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	2	12	theme	non-ST-elevation	257:272	arg1	NSTE-ACS					299:306	NSTE-ACS	299:306	NSTE-ACS	299:306	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	5	13	theme	patients	592:599	arg1	data					575:578	prospective multicenter registry data	542:578	prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017	542:681	METHODS Analysis of prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017.					
33667576	4	14	theme	contemporary	485:496	arg1	management					498:507	the contemporary management	481:507	the contemporary management of NSTE-ACS	481:519	We aimed to assess the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS.					
33667576	3	15	theme	conflicting	319:329	arg1	findings					331:338	conflicting findings	319:338	conflicting findings	319:338	However, conflicting findings exist whether this holds true in current clinical practice.					
33667576	6	16	theme	competing	709:717	arg1	risks					719:723	competing risks	709:723	a competing risks framework including site-specific random effects	707:772	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	5	17	theme	registry	566:573	arg1	data					575:578	prospective multicenter registry data	542:578	prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017	542:681	METHODS Analysis of prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017.					
33667576	12	18	from	CONCLUSIONS	1710:1720	arg1	management					1738:1747	contemporary management	1725:1747	contemporary management of NSTE-ACS	1725:1759	CONCLUSIONS In contemporary management of NSTE-ACS, fondaparinux seems to provide a favorable net clinical benefit compared with enoxaparin, primarily driven by a bleeding reduction.					
33667576	5	19	dep	METHODS	522:528	arg1	Analysis					530:537	Analysis	530:537	METHODS Analysis of prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017.	522:682	METHODS Analysis of prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017.					
33667576	13	20	theme	access	1967:1972	arg1	approach					1974:1981	the vascular access approach	1954:1981	the vascular access approach	1954:1981	Effect modification on the safety profile of fondaparinux by the vascular access approach warrants further investigation.					
33667576	0	21	theme	non-ST-elevation	65:80	arg1	syndromes					97:105	non-ST-elevation acute coronary syndromes	65:105	non-ST-elevation acute coronary syndromes	65:105	Fondaparinux versus enoxaparin in the contemporary management of non-ST-elevation acute coronary syndromes.					
33667576	2	22	theme	coronary	280:287	arg1	syndrome					289:296	non-ST-elevation acute coronary syndrome	257:296	non-ST-elevation acute coronary syndrome (NSTE-ACS)	257:307	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	2	22	theme	coronary	280:287	arg1	NSTE-ACS					299:306	NSTE-ACS	299:306	NSTE-ACS	299:306	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	6	23	theme	Survival	684:691	arg1	models					693:698	Survival models	684:698	Survival models	684:698	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	0	24	theme	coronary	88:95	arg1	syndromes					97:105	non-ST-elevation acute coronary syndromes	65:105	non-ST-elevation acute coronary syndromes	65:105	Fondaparinux versus enoxaparin in the contemporary management of non-ST-elevation acute coronary syndromes.					
33667576	8	25	theme	renal	1053:1057	arg1	impairment					1059:1068	renal impairment	1053:1068	renal impairment	1053:1068	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	12	26	theme	contemporary	1725:1736	arg1	management					1738:1747	contemporary management	1725:1747	contemporary management of NSTE-ACS	1725:1759	CONCLUSIONS In contemporary management of NSTE-ACS, fondaparinux seems to provide a favorable net clinical benefit compared with enoxaparin, primarily driven by a bleeding reduction.					
33667576	0	27	from	Fondaparinux	0:11	arg1	management					51:60	the contemporary management	34:60	the contemporary management of non-ST-elevation acute coronary syndromes	34:105	Fondaparinux versus enoxaparin in the contemporary management of non-ST-elevation acute coronary syndromes.					
33667576	8	28	theme	lower	1020:1024	arg1	prevalence					1026:1035	a lower prevalence	1018:1035	a lower prevalence of diabetes and renal impairment	1018:1068	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	13	29	theme	fondaparinux	1938:1949	arg1	profile					1927:1933	the safety profile	1916:1933	the safety profile of fondaparinux	1916:1949	Effect modification on the safety profile of fondaparinux by the vascular access approach warrants further investigation.					
33667576	3	30	theme	current	373:379	arg1	practice					390:397	current clinical practice	373:397	current clinical practice	373:397	However, conflicting findings exist whether this holds true in current clinical practice.					
33667576	8	31	theme	greater	1075:1081	arg1	use					1083:1085	greater use	1075:1085	greater use of transradial approach in the fondaparinux group	1075:1135	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	9	32	dep	CI	1338:1339	arg1	SHR					1325:1327	SHR 0.57	1325:1332	SHR 0.57	1325:1332	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	10	33	theme	transradial	1459:1469	arg1	approach					1471:1478	transradial approach	1459:1478	transradial approach	1459:1478	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	13	34	theme	further	1992:1998	arg1	investigation					2000:2012	further investigation	1992:2012	further investigation	1992:2012	Effect modification on the safety profile of fondaparinux by the vascular access approach warrants further investigation.					
33667576	1	35	from	multicenter	124:134	arg1	Insights					108:115	Insights	108:115	Insights from a multicenter	108:134	Insights from a multicenter registry.					
33667576	6	36	from	30 days	885:891	arg1	composite					799:807	the composite	795:807	the composite of clinically relevant bleedings	795:840	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	6	36	from	30 days	885:891	arg1	events					875:880	major adverse cardiovascular events	846:880	major adverse cardiovascular events at 30 days	846:891	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	6	36	from	30 days	885:891	arg1	bleedings					832:840	clinically relevant bleedings	812:840	clinically relevant bleedings	812:840	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	12	37	theme	net	1804:1806	arg1	benefit					1817:1823	a favorable net clinical benefit	1792:1823	a favorable net clinical benefit	1792:1823	CONCLUSIONS In contemporary management of NSTE-ACS, fondaparinux seems to provide a favorable net clinical benefit compared with enoxaparin, primarily driven by a bleeding reduction.					
33667576	6	38	theme	site-specific	745:757	arg1	effects					766:772	site-specific random effects	745:772	site-specific random effects	745:772	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	8	39	theme	approach	1102:1109	arg1	use					1083:1085	greater use	1075:1085	greater use of transradial approach in the fondaparinux group	1075:1135	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	8	39	theme	approach	1102:1109	arg1	prevalence					1026:1035	a lower prevalence	1018:1035	a lower prevalence of diabetes and renal impairment	1018:1068	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	6	40	used	used	780:783	arg2	models					693:698	Survival models	684:698	Survival models	684:698	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	10	41	theme	vascular	1539:1546	arg1	access					1548:1553	vascular access	1539:1553	vascular access	1539:1553	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	6	42	theme	relevant	823:830	arg1	bleedings					832:840	clinically relevant bleedings	812:840	clinically relevant bleedings	812:840	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	9	43	theme	Subhazard	1237:1245	arg1	enoxaparin					1225:1234	enoxaparin	1225:1234	enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92)	1225:1280	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	9	43	theme	Subhazard	1237:1245	arg1	CI					1268:1269	Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92	1237:1279	Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92	1237:1279	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	9	44	theme	clinical	1175:1182	arg1	benefit					1184:1190	a net clinical benefit	1169:1190	a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89)	1169:1350	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	10	45	theme	multiplicative	1562:1575	arg1	Pinteraction = 0.0056					1584:1604	Pinteraction = 0.0056	1584:1604	Pinteraction = 0.0056	1584:1604	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	10	45	theme	multiplicative	1562:1575	arg1	scale					1577:1581	the multiplicative scale	1558:1581	the multiplicative scale (Pinteraction = 0.0056)	1558:1605	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	2	46	theme	risk-benefit	208:219	arg1	profile					221:227	the most favorable risk-benefit profile	189:227	the most favorable risk-benefit profile	189:227	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	7	47	theme	370	955:957	arg1	fondaparinux					965:976	370 (18%) fondaparinux	955:976	370 (18%) fondaparinux	955:976	RESULTS Of 2094 patients, 1724 (82%) received enoxaparin and 370 (18%) fondaparinux.					
33667576	13	48	from	modification	1900:1911	arg1	profile					1927:1933	the safety profile	1916:1933	the safety profile of fondaparinux	1916:1949	Effect modification on the safety profile of fondaparinux by the vascular access approach warrants further investigation.					
33667576	11	49	theme	Propensity-score-matching	1650:1674	arg1	analysis					1676:1683	Propensity-score-matching analysis	1650:1683	Propensity-score-matching analysis	1650:1683	Propensity-score-matching analysis yielded similar results.					
33667576	4	50	theme	net	423:425	arg1	benefit					436:442	the net clinical benefit	419:442	the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS	419:519	We aimed to assess the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS.					
33667576	8	51	from	prevalence	1026:1035	arg1	group					1131:1135	the fondaparinux group	1114:1135	the fondaparinux group	1114:1135	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	9	52	theme	Ratio	1247:1251	arg1	enoxaparin					1225:1234	enoxaparin	1225:1234	enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92)	1225:1280	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	9	52	theme	Ratio	1247:1251	arg1	CI					1268:1269	Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92	1237:1279	Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92	1237:1279	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	12	53	theme	clinical	1808:1815	arg1	benefit					1817:1823	a favorable net clinical benefit	1792:1823	a favorable net clinical benefit	1792:1823	CONCLUSIONS In contemporary management of NSTE-ACS, fondaparinux seems to provide a favorable net clinical benefit compared with enoxaparin, primarily driven by a bleeding reduction.					
33667576	2	54	contain	have	184:187	arg2	profile					221:227	the most favorable risk-benefit profile	189:227	the most favorable risk-benefit profile	189:227	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	2	54	contain	have	184:187	arg1	Fondaparinux					157:168	BACKGROUND Fondaparinux	146:168	BACKGROUND Fondaparinux	146:168	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	6	55	theme	adverse	852:858	arg1	events					875:880	major adverse cardiovascular events	846:880	major adverse cardiovascular events at 30 days	846:891	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	5	56	theme	multicenter	554:564	arg1	data					575:578	prospective multicenter registry data	542:578	prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017	542:681	METHODS Analysis of prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017.					
33667576	6	57	theme	major	846:850	arg1	events					875:880	major adverse cardiovascular events	846:880	major adverse cardiovascular events at 30 days	846:891	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	10	58	theme	greater	1384:1390	arg1	reductions					1392:1401	greater reductions	1384:1401	greater reductions in bleeding	1384:1413	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	7	59	theme	patients	910:917	arg1	RESULTS					894:900	RESULTS	894:900	RESULTS Of 2094 patients, 1724 (82%)	894:929	RESULTS Of 2094 patients, 1724 (82%) received enoxaparin and 370 (18%) fondaparinux.					
33667576	2	60	theme	acute	274:278	arg1	syndrome					289:296	non-ST-elevation acute coronary syndrome	257:296	non-ST-elevation acute coronary syndrome (NSTE-ACS)	257:307	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	2	60	theme	acute	274:278	arg1	NSTE-ACS					299:306	NSTE-ACS	299:306	NSTE-ACS	299:306	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	10	61	theme	additive	1622:1629	arg1	P = 0.457					1638:1646	P = 0.457	1638:1646	P = 0.457	1638:1646	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	10	61	theme	additive	1622:1629	arg1	scale					1631:1635	an additive scale	1619:1635	an additive scale (P = 0.457)	1619:1647	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	9	62	theme	%	1267:1267	arg1	enoxaparin					1225:1234	enoxaparin	1225:1234	enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92)	1225:1280	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	9	62	theme	%	1267:1267	arg1	CI					1268:1269	Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92	1237:1279	Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92	1237:1279	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	8	63	from	use	1083:1085	arg1	group					1131:1135	the fondaparinux group	1114:1135	the fondaparinux group	1114:1135	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	4	64	theme	fondaparinux	447:458	arg1	benefit					436:442	the net clinical benefit	419:442	the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS	419:519	We aimed to assess the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS.					
33667576	6	65	theme	risks	719:723	arg1	framework					725:733	a competing risks framework	707:733	a competing risks framework including site-specific random effects	707:772	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	10	66	theme	Exploratory	1353:1363	arg1	analysis					1365:1372	Exploratory analysis	1353:1372	Exploratory analysis	1353:1372	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	4	67	theme	enoxaparin	467:476	arg1	benefit					436:442	the net clinical benefit	419:442	the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS	419:519	We aimed to assess the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS.					
33667576	13	68	theme	vascular	1958:1965	arg1	approach					1974:1981	the vascular access approach	1954:1981	the vascular access approach	1954:1981	Effect modification on the safety profile of fondaparinux by the vascular access approach warrants further investigation.					
33667576	0	69	theme	acute	82:86	arg1	syndromes					97:105	non-ST-elevation acute coronary syndromes	65:105	non-ST-elevation acute coronary syndromes	65:105	Fondaparinux versus enoxaparin in the contemporary management of non-ST-elevation acute coronary syndromes.					
33667576	5	70	theme	data	575:578	arg1	Analysis					530:537	Analysis	530:537	METHODS Analysis of prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017.	522:682	METHODS Analysis of prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017.					
33667576	12	71	theme	NSTE-ACS	1752:1759	arg1	management					1738:1747	contemporary management	1725:1747	contemporary management of NSTE-ACS	1725:1759	CONCLUSIONS In contemporary management of NSTE-ACS, fondaparinux seems to provide a favorable net clinical benefit compared with enoxaparin, primarily driven by a bleeding reduction.					
33667576	10	72	from	interaction	1505:1515	arg1	Pinteraction = 0.0056					1584:1604	Pinteraction = 0.0056	1584:1604	Pinteraction = 0.0056	1584:1604	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	10	72	from	interaction	1505:1515	arg1	P = 0.457					1638:1646	P = 0.457	1638:1646	P = 0.457	1638:1646	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	10	72	from	interaction	1505:1515	arg1	scale					1631:1635	an additive scale	1619:1635	an additive scale (P = 0.457)	1619:1647	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	10	72	from	interaction	1505:1515	arg1	scale					1577:1581	the multiplicative scale	1558:1581	the multiplicative scale (Pinteraction = 0.0056)	1558:1605	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	13	73	theme	safety	1920:1925	arg1	profile					1927:1933	the safety profile	1916:1933	the safety profile of fondaparinux	1916:1949	Effect modification on the safety profile of fondaparinux by the vascular access approach warrants further investigation.					
33667576	0	74	theme	syndromes	97:105	arg1	management					51:60	the contemporary management	34:60	the contemporary management of non-ST-elevation acute coronary syndromes	34:105	Fondaparinux versus enoxaparin in the contemporary management of non-ST-elevation acute coronary syndromes.					
33667576	8	75	theme	impairment	1059:1068	arg1	use					1083:1085	greater use	1075:1085	greater use of transradial approach in the fondaparinux group	1075:1135	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	8	75	theme	impairment	1059:1068	arg1	prevalence					1026:1035	a lower prevalence	1018:1035	a lower prevalence of diabetes and renal impairment	1018:1068	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	5	76	theme	NSTE-ACS	583:590	arg1	patients					592:599	NSTE-ACS patients	583:599	NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017	583:681	METHODS Analysis of prospective multicenter registry data of NSTE-ACS patients who received fondaparinux or enoxaparin from February 2015, through December 2017.					
33667576	4	77	theme	NSTE-ACS	512:519	arg1	management					498:507	the contemporary management	481:507	the contemporary management of NSTE-ACS	481:519	We aimed to assess the net clinical benefit of fondaparinux versus enoxaparin in the contemporary management of NSTE-ACS.					
33667576	13	78	theme	Effect	1893:1898	arg1	modification					1900:1911	Effect modification	1893:1911	Effect modification on the safety profile of fondaparinux by the vascular access approach	1893:1981	Effect modification on the safety profile of fondaparinux by the vascular access approach warrants further investigation.					
33667576	2	79	from	anticoagulants	239:252	arg1	syndrome					289:296	non-ST-elevation acute coronary syndrome	257:296	non-ST-elevation acute coronary syndrome (NSTE-ACS)	257:307	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	2	79	from	anticoagulants	239:252	arg1	NSTE-ACS					299:306	NSTE-ACS	299:306	NSTE-ACS	299:306	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	3	80	theme	clinical	381:388	arg1	practice					390:397	current clinical practice	373:397	current clinical practice	373:397	However, conflicting findings exist whether this holds true in current clinical practice.					
33667576	8	81	theme	transradial	1090:1100	arg1	approach					1102:1109	transradial approach	1090:1109	transradial approach	1090:1109	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	6	82	theme	random	759:764	arg1	effects					766:772	site-specific random effects	745:772	site-specific random effects	745:772	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	10	83	theme	significant	1493:1503	arg1	interaction					1505:1515	a significant interaction	1491:1515	a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457)	1491:1647	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	2	84	theme	favorable	198:206	arg1	profile					221:227	the most favorable risk-benefit profile	189:227	the most favorable risk-benefit profile	189:227	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
33667576	9	85	theme	Multivariate	1138:1149	arg1	analysis					1151:1158	Multivariate analysis	1138:1158	Multivariate analysis	1138:1158	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	0	86	theme	contemporary	38:49	arg1	management					51:60	the contemporary management	34:60	the contemporary management of non-ST-elevation acute coronary syndromes	34:105	Fondaparinux versus enoxaparin in the contemporary management of non-ST-elevation acute coronary syndromes.					
33667576	8	87	theme	fondaparinux	1118:1129	arg1	group					1131:1135	the fondaparinux group	1114:1135	the fondaparinux group	1114:1135	Both groups were comparable except for a lower prevalence of diabetes and renal impairment, and greater use of transradial approach in the fondaparinux group.					
33667576	9	88	dep	bleeding	1315:1322	arg1	CI					1338:1339	CI	1338:1339	CI	1338:1339	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	6	89	theme	bleedings	832:840	arg1	composite					799:807	the composite	795:807	the composite of clinically relevant bleedings	795:840	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	6	89	theme	bleedings	832:840	arg1	events					875:880	major adverse cardiovascular events	846:880	major adverse cardiovascular events at 30 days	846:891	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	6	89	theme	bleedings	832:840	arg1	bleedings					832:840	clinically relevant bleedings	812:840	clinically relevant bleedings	812:840	Survival models within a competing risks framework including site-specific random effects, were used to assess the composite of clinically relevant bleedings and major adverse cardiovascular events at 30 days.					
33667576	12	90	theme	bleeding	1873:1880	arg1	reduction					1882:1890	a bleeding reduction	1871:1890	a bleeding reduction	1871:1890	CONCLUSIONS In contemporary management of NSTE-ACS, fondaparinux seems to provide a favorable net clinical benefit compared with enoxaparin, primarily driven by a bleeding reduction.					
33667576	9	91	theme	net	1171:1173	arg1	benefit					1184:1190	a net clinical benefit	1169:1190	a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89)	1169:1350	Multivariate analysis revealed a net clinical benefit in favour of fondaparinux versus enoxaparin (Subhazard Ratio [SHR] 0.59; 95%CI 0.37-0.92), mainly driven by a reduction in bleeding (SHR 0.57; 95%CI 0.37-0.89).					
33667576	10	92	from	reductions	1392:1401	arg1	bleeding					1406:1413	bleeding	1406:1413	bleeding	1406:1413	Exploratory analysis suggested greater reductions in bleeding with fondaparinux among patients undergoing transradial approach, revealing a significant interaction between treatment and vascular access on the multiplicative scale (Pinteraction = 0.0056), but not on an additive scale (P = 0.457).					
33667576	2	93	theme	BACKGROUND	146:155	arg1	Fondaparinux					157:168	BACKGROUND Fondaparinux	146:168	BACKGROUND Fondaparinux	146:168	BACKGROUND Fondaparinux is thought to have the most favorable risk-benefit profile among all anticoagulants in non-ST-elevation acute coronary syndrome (NSTE-ACS).					
34890608	1	0	theme	thermo-chemical	359:373	arg1	process					385:391	thermo-chemical oxidation process	359:391	thermo-chemical oxidation process using sodium hydroxide	359:414	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	7	1	theme	donor	1211:1215	arg1	interaction					1248:1258	hydrophobic interaction	1236:1258	hydrophobic interaction	1236:1258	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	1	theme	donor	1211:1215	arg1	formation					1291:1299	charge assisted hydrogen bond formation	1261:1299	charge assisted hydrogen bond formation	1261:1299	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	1	theme	donor	1211:1215	arg1	EDA					1230:1232	EDA	1230:1232	EDA	1230:1232	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	1	theme	donor	1211:1215	arg1	interaction					1217:1227	π-π electron donor interaction	1198:1227	π-π electron donor interaction (EDA)	1198:1233	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	1	2	with	carbonization	264:276	arg1	nonahydrate					323:333	Iron (III) nitrate nonahydrate	304:333	Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide	304:414	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	1	3	theme	bagasse	291:297	arg1	carbonization					264:276	hydrothermal carbonization	251:276	hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide	251:414	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	1	4	theme	oxidation	375:383	arg1	process					385:391	thermo-chemical oxidation process	359:391	thermo-chemical oxidation process using sodium hydroxide	359:414	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	7	5	theme	electron	1202:1209	arg1	interaction					1248:1258	hydrophobic interaction	1236:1258	hydrophobic interaction	1236:1258	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	5	theme	electron	1202:1209	arg1	formation					1291:1299	charge assisted hydrogen bond formation	1261:1299	charge assisted hydrogen bond formation	1261:1299	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	5	theme	electron	1202:1209	arg1	EDA					1230:1232	EDA	1230:1232	EDA	1230:1232	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	5	theme	electron	1202:1209	arg1	interaction					1217:1227	π-π electron donor interaction	1198:1227	π-π electron donor interaction (EDA)	1198:1233	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	0	6	theme	bagasse	110:116	arg1	carbonization					83:95	hydrothermal carbonization	70:95	hydrothermal carbonization of sugarcane bagasse	70:116	Facile route for synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite using hydrothermal carbonization of sugarcane bagasse and its use as effective adsorbent for sulfamethoxazole removal.					
34890608	0	6	theme	bagasse	110:116	arg1	use					126:128	its use	122:128	its use as effective adsorbent for sulfamethoxazole removal	122:180	Facile route for synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite using hydrothermal carbonization of sugarcane bagasse and its use as effective adsorbent for sulfamethoxazole removal.					
34890608	7	7	theme	π-π	1198:1200	arg1	interaction					1248:1258	hydrophobic interaction	1236:1258	hydrophobic interaction	1236:1258	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	7	theme	π-π	1198:1200	arg1	formation					1291:1299	charge assisted hydrogen bond formation	1261:1299	charge assisted hydrogen bond formation	1261:1299	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	7	theme	π-π	1198:1200	arg1	EDA					1230:1232	EDA	1230:1232	EDA	1230:1232	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	7	theme	π-π	1198:1200	arg1	interaction					1217:1227	π-π electron donor interaction	1198:1227	π-π electron donor interaction (EDA)	1198:1233	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	1	8	theme	Iron	304:307	arg1	nonahydrate					323:333	Iron (III) nitrate nonahydrate	304:333	Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide	304:414	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	0	9	theme	sugarcane	100:108	arg1	bagasse					110:116	sugarcane bagasse	100:116	sugarcane bagasse	100:116	Facile route for synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite using hydrothermal carbonization of sugarcane bagasse and its use as effective adsorbent for sulfamethoxazole removal.					
34890608	4	10	theme	carbon	844:849	arg1	matrix					851:856	the carbon matrix	840:856	the carbon matrix	840:856	Textural studies showed that after thermo-chemical activation intensified the surface area on composites to about 491.474 m2 g-1 thereby, promoting improved porosity on the carbon matrix.					
34890608	3	11	theme	spherical	605:613	arg1	carbon					622:627	the spherical shaped carbon	601:627	the spherical shaped carbon	601:627	SEM analysis showed the spherical shaped carbon is well encapsulated with iron particles.					
34890608	4	12	from	area	757:760	arg1	composites					765:774	composites	765:774	composites	765:774	Textural studies showed that after thermo-chemical activation intensified the surface area on composites to about 491.474 m2 g-1 thereby, promoting improved porosity on the carbon matrix.					
34890608	9	13	theme	high	1515:1518	arg1	level					1520:1524	the material to high level	1499:1524	the material to high level due to their unique property	1499:1553	Use of magnetic carbon helps in industrialising the material to high level due to their unique property in separation and recycling.					
34890608	7	14	theme	assisted	1268:1275	arg1	bond					1286:1289	charge assisted hydrogen bond	1261:1289	charge assisted hydrogen bond formation	1261:1299	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	1	15	theme	sodium	399:404	arg1	hydroxide					406:414	sodium hydroxide	399:414	sodium hydroxide	399:414	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	5	16	theme	SMX	926:928	arg1	capacity					941:948	SMX adsorption capacity	926:948	SMX adsorption capacity	926:948	The fabricated composite showed magnetization of 32.84 emu g-1 and SMX adsorption capacity as 169.49 mg g-1.					
34890608	8	17	theme	pollutant	1440:1448	arg1	presence					1419:1426	the presence	1415:1426	the presence of multiple pollutant	1415:1448	The selectivity nature of magnetic composite with respect to SMX was studied in the presence of multiple pollutant.					
34890608	7	18	from	mode	1087:1090	arg1	composites					1133:1142	magnetic carbon composites	1117:1142	magnetic carbon composites with respect to different pH	1117:1171	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	1	19	theme	Non-toxic	183:191	arg1	composite					220:228	Non-toxic mesoporous magnetic carbon composite	183:228	Non-toxic mesoporous magnetic carbon composite	183:228	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	2	20	theme	XRD	498:500	arg1	analysis					502:509	XRD analysis	498:509	XRD analysis	498:509	IR spectrum exposed the presence of oxygenated functional groups and FeO whereas XRD analysis revealed the availability of Fe0/Fe3C/γ-Fe2O3 at varying intensities.					
34890608	6	21	theme	isotherm	981:988	arg1	studies					990:996	Kinetics and isotherm studies	968:996	Kinetics and isotherm studies	968:996	Kinetics and isotherm studies revealed that removal of SMX fitted well in Pseudo-second order and Langmuir models.					
34890608	1	22	from	nonahydrate	323:333	arg1	steps					342:346	two steps	338:346	two steps along with thermo-chemical oxidation process using sodium hydroxide	338:414	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	5	23	theme	32.84	908:912	arg1	g-1					918:920	32.84 emu g-1	908:920	32.84 emu g-1	908:920	The fabricated composite showed magnetization of 32.84 emu g-1 and SMX adsorption capacity as 169.49 mg g-1.					
34890608	1	24	theme	mesoporous	193:202	arg1	composite					220:228	Non-toxic mesoporous magnetic carbon composite	183:228	Non-toxic mesoporous magnetic carbon composite	183:228	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	10	25	theme	carbon	1704:1709	arg1	compound					1711:1718	structural carbon compound	1693:1718	structural carbon compound	1693:1718	Application of hydrothermal carbonization is found to be applicable for wet solid substance thereby evolving structural carbon compound.					
34890608	10	26	theme	solid	1660:1664	arg1	substance					1666:1674	wet solid substance	1656:1674	wet solid substance	1656:1674	Application of hydrothermal carbonization is found to be applicable for wet solid substance thereby evolving structural carbon compound.					
34890608	10	27	theme	carbonization	1612:1624	arg1	Application					1584:1594	Application	1584:1594	Application of hydrothermal carbonization	1584:1624	Application of hydrothermal carbonization is found to be applicable for wet solid substance thereby evolving structural carbon compound.					
34890608	1	28	theme	magnetic	204:211	arg1	composite					220:228	Non-toxic mesoporous magnetic carbon composite	183:228	Non-toxic mesoporous magnetic carbon composite	183:228	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	0	29	theme	Facile	0:5	arg1	route					7:11	Facile route	0:11	Facile route for synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite	0:62	Facile route for synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite using hydrothermal carbonization of sugarcane bagasse and its use as effective adsorbent for sulfamethoxazole removal.					
34890608	4	30	from	porosity	828:835	arg1	matrix					851:856	the carbon matrix	840:856	the carbon matrix	840:856	Textural studies showed that after thermo-chemical activation intensified the surface area on composites to about 491.474 m2 g-1 thereby, promoting improved porosity on the carbon matrix.					
34890608	4	31	theme	improved	819:826	arg1	porosity					828:835	improved porosity	819:835	improved porosity on the carbon matrix	819:856	Textural studies showed that after thermo-chemical activation intensified the surface area on composites to about 491.474 m2 g-1 thereby, promoting improved porosity on the carbon matrix.					
34890608	1	32	theme	carbon	213:218	arg1	composite					220:228	Non-toxic mesoporous magnetic carbon composite	183:228	Non-toxic mesoporous magnetic carbon composite	183:228	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	5	33	theme	emu	914:916	arg1	g-1					918:920	32.84 emu g-1	908:920	32.84 emu g-1	908:920	The fabricated composite showed magnetization of 32.84 emu g-1 and SMX adsorption capacity as 169.49 mg g-1.					
34890608	6	34	theme	Langmuir	1066:1073	arg1	models					1075:1080	Langmuir models	1066:1080	Langmuir models	1066:1080	Kinetics and isotherm studies revealed that removal of SMX fitted well in Pseudo-second order and Langmuir models.					
34890608	7	35	theme	different	1160:1168	arg1	pH					1170:1171	different pH	1160:1171	different pH	1160:1171	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	3	36	theme	shaped	615:620	arg1	carbon					622:627	the spherical shaped carbon	601:627	the spherical shaped carbon	601:627	SEM analysis showed the spherical shaped carbon is well encapsulated with iron particles.					
34890608	2	37	theme	functional	464:473	arg1	groups					475:480	oxygenated functional groups	453:480	oxygenated functional groups	453:480	IR spectrum exposed the presence of oxygenated functional groups and FeO whereas XRD analysis revealed the availability of Fe0/Fe3C/γ-Fe2O3 at varying intensities.					
34890608	2	38	theme	groups	475:480	arg1	FeO					486:488	FeO	486:488	FeO	486:488	IR spectrum exposed the presence of oxygenated functional groups and FeO whereas XRD analysis revealed the availability of Fe0/Fe3C/γ-Fe2O3 at varying intensities.					
34890608	2	38	theme	groups	475:480	arg1	presence					441:448	the presence	437:448	the presence of oxygenated functional groups	437:480	IR spectrum exposed the presence of oxygenated functional groups and FeO whereas XRD analysis revealed the availability of Fe0/Fe3C/γ-Fe2O3 at varying intensities.					
34890608	1	39	theme	nitrate	315:321	arg1	nonahydrate					323:333	Iron (III) nitrate nonahydrate	304:333	Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide	304:414	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	4	40	theme	Textural	671:678	arg1	studies					680:686	Textural studies	671:686	Textural studies	671:686	Textural studies showed that after thermo-chemical activation intensified the surface area on composites to about 491.474 m2 g-1 thereby, promoting improved porosity on the carbon matrix.					
34890608	6	41	theme	Kinetics	968:975	arg1	studies					990:996	Kinetics and isotherm studies	968:996	Kinetics and isotherm studies	968:996	Kinetics and isotherm studies revealed that removal of SMX fitted well in Pseudo-second order and Langmuir models.					
34890608	7	42	theme	carbon	1126:1131	arg1	composites					1133:1142	magnetic carbon composites	1117:1142	magnetic carbon composites with respect to different pH	1117:1171	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	2	43	theme	oxygenated	453:462	arg1	groups					475:480	oxygenated functional groups	453:480	oxygenated functional groups	453:480	IR spectrum exposed the presence of oxygenated functional groups and FeO whereas XRD analysis revealed the availability of Fe0/Fe3C/γ-Fe2O3 at varying intensities.					
34890608	0	44	theme	Fe0/Fe3C/γ-Fe2O3	30:45	arg1	composite					54:62	Fe0/Fe3C/γ-Fe2O3 carbon composite	30:62	Fe0/Fe3C/γ-Fe2O3 carbon composite	30:62	Facile route for synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite using hydrothermal carbonization of sugarcane bagasse and its use as effective adsorbent for sulfamethoxazole removal.					
34890608	7	45	theme	charge	1261:1266	arg1	bond					1286:1289	charge assisted hydrogen bond	1261:1289	charge assisted hydrogen bond formation	1261:1299	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	3	46	theme	iron	655:658	arg1	particles					660:668	iron particles	655:668	iron particles	655:668	SEM analysis showed the spherical shaped carbon is well encapsulated with iron particles.					
34890608	2	47	theme	IR	417:418	arg1	spectrum					420:427	IR spectrum	417:427	IR spectrum	417:427	IR spectrum exposed the presence of oxygenated functional groups and FeO whereas XRD analysis revealed the availability of Fe0/Fe3C/γ-Fe2O3 at varying intensities.					
34890608	5	48	theme	adsorption	930:939	arg1	capacity					941:948	SMX adsorption capacity	926:948	SMX adsorption capacity	926:948	The fabricated composite showed magnetization of 32.84 emu g-1 and SMX adsorption capacity as 169.49 mg g-1.					
34890608	9	49	theme	carbon	1467:1472	arg1	Use					1451:1453	Use	1451:1453	Use of magnetic carbon	1451:1472	Use of magnetic carbon helps in industrialising the material to high level due to their unique property in separation and recycling.					
34890608	6	50	theme	Pseudo-second	1042:1054	arg1	order					1056:1060	Pseudo-second order	1042:1060	Pseudo-second order	1042:1060	Kinetics and isotherm studies revealed that removal of SMX fitted well in Pseudo-second order and Langmuir models.					
34890608	8	51	theme	composite	1370:1378	arg1	nature					1351:1356	The selectivity nature	1335:1356	The selectivity nature of magnetic composite with respect to SMX	1335:1398	The selectivity nature of magnetic composite with respect to SMX was studied in the presence of multiple pollutant.					
34890608	7	52	theme	hydrophobic	1236:1246	arg1	interaction					1248:1258	hydrophobic interaction	1236:1258	hydrophobic interaction	1236:1258	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	52	theme	hydrophobic	1236:1246	arg1	interaction					1217:1227	π-π electron donor interaction	1198:1227	π-π electron donor interaction (EDA)	1198:1233	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	5	53	theme	capacity	941:948	arg1	magnetization					891:903	magnetization	891:903	magnetization of 32.84 emu g-1 and SMX adsorption capacity	891:948	The fabricated composite showed magnetization of 32.84 emu g-1 and SMX adsorption capacity as 169.49 mg g-1.					
34890608	2	54	theme	varying	560:566	arg1	intensities					568:578	varying intensities	560:578	varying intensities	560:578	IR spectrum exposed the presence of oxygenated functional groups and FeO whereas XRD analysis revealed the availability of Fe0/Fe3C/γ-Fe2O3 at varying intensities.					
34890608	0	55	theme	composite	54:62	arg1	synthesis					17:25	synthesis	17:25	synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite	17:62	Facile route for synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite using hydrothermal carbonization of sugarcane bagasse and its use as effective adsorbent for sulfamethoxazole removal.					
34890608	7	56	theme	bond	1286:1289	arg1	formation					1291:1299	charge assisted hydrogen bond formation	1261:1299	charge assisted hydrogen bond formation	1261:1299	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	56	theme	bond	1286:1289	arg1	interaction					1217:1227	π-π electron donor interaction	1198:1227	π-π electron donor interaction (EDA)	1198:1233	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	0	57	theme	sulfamethoxazole	157:172	arg1	removal					174:180	sulfamethoxazole removal	157:180	sulfamethoxazole removal	157:180	Facile route for synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite using hydrothermal carbonization of sugarcane bagasse and its use as effective adsorbent for sulfamethoxazole removal.					
34890608	7	58	theme	SMX	1322:1324	arg1	removal					1326:1332	SMX removal	1322:1332	SMX removal	1322:1332	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	10	59	theme	hydrothermal	1599:1610	arg1	carbonization					1612:1624	hydrothermal carbonization	1599:1624	hydrothermal carbonization	1599:1624	Application of hydrothermal carbonization is found to be applicable for wet solid substance thereby evolving structural carbon compound.					
34890608	0	60	theme	carbon	47:52	arg1	composite					54:62	Fe0/Fe3C/γ-Fe2O3 carbon composite	30:62	Fe0/Fe3C/γ-Fe2O3 carbon composite	30:62	Facile route for synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite using hydrothermal carbonization of sugarcane bagasse and its use as effective adsorbent for sulfamethoxazole removal.					
34890608	7	61	theme	hydrogen	1277:1284	arg1	bond					1286:1289	charge assisted hydrogen bond	1261:1289	charge assisted hydrogen bond formation	1261:1299	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	7	62	theme	interaction	1095:1105	arg1	mode					1087:1090	The mode	1083:1090	The mode of interaction of SMX on magnetic carbon composites with respect to different pH	1083:1171	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	4	63	theme	surface	749:755	arg1	area					757:760	the surface area	745:760	the surface area on composites to about 491.474 m2 g-1	745:798	Textural studies showed that after thermo-chemical activation intensified the surface area on composites to about 491.474 m2 g-1 thereby, promoting improved porosity on the carbon matrix.					
34890608	10	64	theme	structural	1693:1702	arg1	compound					1711:1718	structural carbon compound	1693:1718	structural carbon compound	1693:1718	Application of hydrothermal carbonization is found to be applicable for wet solid substance thereby evolving structural carbon compound.					
34890608	9	65	theme	magnetic	1458:1465	arg1	carbon					1467:1472	magnetic carbon	1458:1472	magnetic carbon	1458:1472	Use of magnetic carbon helps in industrialising the material to high level due to their unique property in separation and recycling.					
34890608	7	66	theme	magnetic	1117:1124	arg1	composites					1133:1142	magnetic carbon composites	1117:1142	magnetic carbon composites with respect to different pH	1117:1171	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	9	67	dep	high	1515:1518	arg1	to					1512:1513	to	1512:1513	to	1512:1513	Use of magnetic carbon helps in industrialising the material to high level due to their unique property in separation and recycling.					
34890608	8	68	theme	selectivity	1339:1349	arg1	nature					1351:1356	The selectivity nature	1335:1356	The selectivity nature of magnetic composite with respect to SMX	1335:1398	The selectivity nature of magnetic composite with respect to SMX was studied in the presence of multiple pollutant.					
34890608	8	69	theme	multiple	1431:1438	arg1	pollutant					1440:1448	multiple pollutant	1431:1448	multiple pollutant	1431:1448	The selectivity nature of magnetic composite with respect to SMX was studied in the presence of multiple pollutant.					
34890608	1	70	theme	hydrothermal	251:262	arg1	carbonization					264:276	hydrothermal carbonization	251:276	hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide	251:414	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
34890608	5	71	theme	fabricated	863:872	arg1	composite					874:882	The fabricated composite	859:882	The fabricated composite	859:882	The fabricated composite showed magnetization of 32.84 emu g-1 and SMX adsorption capacity as 169.49 mg g-1.					
34890608	7	72	theme	SMX	1110:1112	arg1	interaction					1095:1105	interaction	1095:1105	interaction of SMX	1095:1112	The mode of interaction of SMX on magnetic carbon composites with respect to different pH was studied showing that π-π electron donor interaction (EDA), hydrophobic interaction, charge assisted hydrogen bond formation were responsible for SMX removal.					
34890608	0	73	theme	hydrothermal	70:81	arg1	carbonization					83:95	hydrothermal carbonization	70:95	hydrothermal carbonization of sugarcane bagasse	70:116	Facile route for synthesis of Fe0/Fe3C/γ-Fe2O3 carbon composite using hydrothermal carbonization of sugarcane bagasse and its use as effective adsorbent for sulfamethoxazole removal.					
34890608	4	74	theme	thermo-chemical	706:720	arg1	activation					722:731	thermo-chemical activation	706:731	thermo-chemical activation	706:731	Textural studies showed that after thermo-chemical activation intensified the surface area on composites to about 491.474 m2 g-1 thereby, promoting improved porosity on the carbon matrix.					
34890608	8	75	theme	magnetic	1361:1368	arg1	composite					1370:1378	magnetic composite	1361:1378	magnetic composite	1361:1378	The selectivity nature of magnetic composite with respect to SMX was studied in the presence of multiple pollutant.					
34890608	9	76	theme	unique	1539:1544	arg1	property					1546:1553	their unique property	1533:1553	their unique property	1533:1553	Use of magnetic carbon helps in industrialising the material to high level due to their unique property in separation and recycling.					
34890608	10	77	theme	wet	1656:1658	arg1	substance					1666:1674	wet solid substance	1656:1674	wet solid substance	1656:1674	Application of hydrothermal carbonization is found to be applicable for wet solid substance thereby evolving structural carbon compound.					
34890608	2	78	theme	Fe0/Fe3C/γ-Fe2O3	540:555	arg1	availability					524:535	the availability	520:535	the availability of Fe0/Fe3C/γ-Fe2O3	520:555	IR spectrum exposed the presence of oxygenated functional groups and FeO whereas XRD analysis revealed the availability of Fe0/Fe3C/γ-Fe2O3 at varying intensities.					
34890608	6	79	theme	SMX	1023:1025	arg1	removal					1012:1018	removal	1012:1018	removal of SMX	1012:1025	Kinetics and isotherm studies revealed that removal of SMX fitted well in Pseudo-second order and Langmuir models.					
34890608	5	80	theme	g-1	918:920	arg1	magnetization					891:903	magnetization	891:903	magnetization of 32.84 emu g-1 and SMX adsorption capacity	891:948	The fabricated composite showed magnetization of 32.84 emu g-1 and SMX adsorption capacity as 169.49 mg g-1.					
34890608	3	81	theme	SEM	581:583	arg1	analysis					585:592	SEM analysis	581:592	SEM analysis	581:592	SEM analysis showed the spherical shaped carbon is well encapsulated with iron particles.					
34890608	1	82	theme	sugarcane	281:289	arg1	bagasse					291:297	sugarcane bagasse	281:297	sugarcane bagasse	281:297	Non-toxic mesoporous magnetic carbon composite was synthesised from hydrothermal carbonization of sugarcane bagasse with Iron (III) nitrate nonahydrate in two steps along with thermo-chemical oxidation process using sodium hydroxide.					
32335838	8	0	theme	xylinus	1312:1318	arg1	SGP8					1327:1330	K. xylinus strain SGP8	1309:1330	K. xylinus strain SGP8	1309:1330	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	2	1	theme	cellulose	360:368	arg1	1.82 g L-1					346:355	1.82 g L-1	346:355	1.82 g L-1 of cellulose	346:368	The bacterium was able to produce 1.82 g L-1 of cellulose in the form of pellicle in standard Hestrin-Schramn (HS) medium.					
32335838	8	2	theme	contaminated	1482:1493	arg1	system					1495:1500	the contaminated system	1478:1500	the contaminated system	1478:1500	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	1	3	theme	strain	248:253	arg1	SGP8					255:258	Komagataeibacter xylinus strain SGP8	223:258	Komagataeibacter xylinus strain SGP8	223:258	In the present study, fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose is presented.					
32335838	8	4	theme	K.	1309:1310	arg1	SGP8					1327:1330	K. xylinus strain SGP8	1309:1330	K. xylinus strain SGP8	1309:1330	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	6	5	from	9	1080:1080	arg1	removal					1025:1031	>99% removal	1020:1031	>99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment	1020:1104	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	8	6	theme	levels	1460:1465	arg1	remediation					1439:1449	remediation	1439:1449	remediation of toxic levels of Cd from the contaminated system	1439:1500	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	8	7	theme	above	1260:1264	arg1	results					1266:1272	the above results	1256:1272	the above results	1256:1272	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	8	8	theme	material	1349:1356	arg1	properties					1358:1367	excellent material properties	1339:1367	excellent material properties	1339:1367	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	4	9	from	rise	733:736	arg1	temperature					741:751	temperature	741:751	temperature	741:751	The thermogravimetric analysis (TGA) showed the stability of BNC up to 280 °C, further rise in temperature to 350 °C results in depolymerization of the sample.					
32335838	8	10	theme	toxic	1454:1458	arg1	levels					1460:1465	toxic levels	1454:1465	toxic levels of Cd	1454:1471	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	6	11	theme	10 mg L-1	1044:1052	arg1	removal					1025:1031	>99% removal	1020:1031	>99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment	1020:1104	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	1	12	theme	fermentative	157:168	arg1	production					170:179	fermentative production	157:179	fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose	157:296	In the present study, fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose is presented.					
32335838	3	13	theme	X-ray	530:534	arg1	diffraction					536:546	X-ray diffraction	530:546	X-ray diffraction (XRD)	530:552	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	3	13	theme	X-ray	530:534	arg1	XRD					549:551	XRD	549:551	XRD	549:551	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	2	14	theme	pellicle	385:392	arg1	form					377:380	the form	373:380	the form of pellicle	373:392	The bacterium was able to produce 1.82 g L-1 of cellulose in the form of pellicle in standard Hestrin-Schramn (HS) medium.					
32335838	3	15	dep	microscopy	509:518	arg1	studies					554:560	studies	554:560	studies	554:560	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	6	16	from	pH 5	1068:1071	arg1	removal					1025:1031	>99% removal	1020:1031	>99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment	1020:1104	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	7	17	theme	high	1174:1177	arg1	concentrations					1179:1192	high concentrations	1174:1192	high concentrations of Cd (25 and 50 mg L-1)	1174:1217	Moreover, the composite was also found to be competent in removing high concentrations of Cd (25 and 50 mg L-1) from the solution (69-70%).					
32335838	8	18	theme	modified	1374:1381	arg1	BNC					1383:1385	modified BNC	1374:1385	modified BNC (BNC-CaCO3 composite)	1374:1407	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	8	18	theme	modified	1374:1381	arg1	composite					1398:1406	BNC-CaCO3 composite	1388:1406	BNC-CaCO3 composite	1388:1406	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	4	19	theme	up	711:712	arg1	stability					694:702	the stability	690:702	the stability of BNC up to 280 °C	690:722	The thermogravimetric analysis (TGA) showed the stability of BNC up to 280 °C, further rise in temperature to 350 °C results in depolymerization of the sample.					
32335838	1	20	theme	bacterial	184:192	arg1	BNC					209:211	BNC	209:211	BNC	209:211	In the present study, fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose is presented.					
32335838	1	20	theme	bacterial	184:192	arg1	nanocellulose					194:206	bacterial nanocellulose	184:206	bacterial nanocellulose (BNC)	184:212	In the present study, fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose is presented.					
32335838	1	21	theme	nanocellulose	284:296	arg1	SGP8					255:258	Komagataeibacter xylinus strain SGP8	223:258	Komagataeibacter xylinus strain SGP8	223:258	In the present study, fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose is presented.					
32335838	1	21	theme	nanocellulose	284:296	arg1	characterization					264:279	characterization	264:279	characterization	264:279	In the present study, fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose is presented.					
32335838	0	22	theme	ions	129:132	arg1	removal					115:121	removal	115:121	removal of Cd ions	115:132	Production and characterization of Komagataeibacter xylinus SGP8 nanocellulose and its calcite based composite for removal of Cd ions.					
32335838	6	23	theme	Cd	1057:1058	arg1	10 mg L-1					1044:1052	initial 10 mg L-1	1036:1052	initial 10 mg L-1 of Cd (II)	1036:1063	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	1	24	theme	nanocellulose	194:206	arg1	production					170:179	fermentative production	157:179	fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose	157:296	In the present study, fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose is presented.					
32335838	8	25	theme	excellent	1339:1347	arg1	properties					1358:1367	excellent material properties	1339:1367	excellent material properties	1339:1367	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	0	26	theme	Cd	126:127	arg1	ions					129:132	Cd ions	126:132	Cd ions	126:132	Production and characterization of Komagataeibacter xylinus SGP8 nanocellulose and its calcite based composite for removal of Cd ions.					
32335838	6	27	theme	%	1023:1023	arg1	removal					1025:1031	>99% removal	1020:1031	>99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment	1020:1104	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	0	28	theme	xylinus	52:58	arg1	nanocellulose					65:77	Komagataeibacter xylinus SGP8 nanocellulose	35:77	Komagataeibacter xylinus SGP8 nanocellulose	35:77	Production and characterization of Komagataeibacter xylinus SGP8 nanocellulose and its calcite based composite for removal of Cd ions.					
32335838	6	29	theme	initial	1036:1042	arg1	10 mg L-1					1044:1052	initial 10 mg L-1	1036:1052	initial 10 mg L-1 of Cd (II)	1036:1063	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	3	30	theme	morpho-structural	439:455	arg1	characterization					457:472	The morpho-structural characterization	435:472	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively	435:574	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	3	31	theme	nanofibrillar	585:597	arg1	structure					599:607	nanofibrillar structure	585:607	nanofibrillar structure	585:607	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	4	32	theme	thermogravimetric	650:666	arg1	TGA					678:680	TGA	678:680	TGA	678:680	The thermogravimetric analysis (TGA) showed the stability of BNC up to 280 °C, further rise in temperature to 350 °C results in depolymerization of the sample.					
32335838	4	32	theme	thermogravimetric	650:666	arg1	analysis					668:675	The thermogravimetric analysis	646:675	The thermogravimetric analysis (TGA)	646:681	The thermogravimetric analysis (TGA) showed the stability of BNC up to 280 °C, further rise in temperature to 350 °C results in depolymerization of the sample.					
32335838	2	33	theme	Hestrin-Schramn	406:420	arg1	medium					427:432	standard Hestrin-Schramn (HS) medium	397:432	standard Hestrin-Schramn (HS) medium	397:432	The bacterium was able to produce 1.82 g L-1 of cellulose in the form of pellicle in standard Hestrin-Schramn (HS) medium.					
32335838	0	34	theme	Komagataeibacter	35:50	arg1	nanocellulose					65:77	Komagataeibacter xylinus SGP8 nanocellulose	35:77	Komagataeibacter xylinus SGP8 nanocellulose	35:77	Production and characterization of Komagataeibacter xylinus SGP8 nanocellulose and its calcite based composite for removal of Cd ions.					
32335838	6	35	theme	treatment	1096:1104	arg1	12 h					1088:1091	12 h	1088:1091	12 h of treatment	1088:1104	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	2	36	theme	standard	397:404	arg1	medium					427:432	standard Hestrin-Schramn (HS) medium	397:432	standard Hestrin-Schramn (HS) medium	397:432	The bacterium was able to produce 1.82 g L-1 of cellulose in the form of pellicle in standard Hestrin-Schramn (HS) medium.					
32335838	0	37	theme	nanocellulose	65:77	arg1	calcite					87:93	its calcite	83:93	its calcite	83:93	Production and characterization of Komagataeibacter xylinus SGP8 nanocellulose and its calcite based composite for removal of Cd ions.					
32335838	0	37	theme	nanocellulose	65:77	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of Komagataeibacter xylinus SGP8 nanocellulose and its calcite based composite for removal of Cd ions.					
32335838	0	37	theme	nanocellulose	65:77	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of Komagataeibacter xylinus SGP8 nanocellulose and its calcite based composite for removal of Cd ions.					
32335838	8	38	theme	BNC-CaCO3	1388:1396	arg1	BNC					1383:1385	modified BNC	1374:1385	modified BNC (BNC-CaCO3 composite)	1374:1407	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	8	38	theme	BNC-CaCO3	1388:1396	arg1	composite					1398:1406	BNC-CaCO3 composite	1388:1406	BNC-CaCO3 composite	1388:1406	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	4	39	theme	sample	798:803	arg1	depolymerization					774:789	depolymerization	774:789	depolymerization of the sample	774:803	The thermogravimetric analysis (TGA) showed the stability of BNC up to 280 °C, further rise in temperature to 350 °C results in depolymerization of the sample.					
32335838	3	40	theme	scanning	491:498	arg1	SEM					521:523	SEM	521:523	SEM	521:523	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	3	40	theme	scanning	491:498	arg1	microscopy					509:518	scanning electron microscopy	491:518	scanning electron microscopy (SEM)	491:524	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	0	41	theme	SGP8	60:63	arg1	nanocellulose					65:77	Komagataeibacter xylinus SGP8 nanocellulose	35:77	Komagataeibacter xylinus SGP8 nanocellulose	35:77	Production and characterization of Komagataeibacter xylinus SGP8 nanocellulose and its calcite based composite for removal of Cd ions.					
32335838	8	42	theme	strain	1320:1325	arg1	SGP8					1327:1330	K. xylinus strain SGP8	1309:1330	K. xylinus strain SGP8	1309:1330	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	5	43	theme	TGA	957:959	arg1	studies					961:967	TGA studies	957:967	TGA studies	957:967	In order to show the applicability of produced BNC, it was modified first using calcite (CaCO3) and thereafter characterized using SEM, XRD, FTIR, and TGA studies.					
32335838	3	44	theme	electron	500:507	arg1	SEM					521:523	SEM	521:523	SEM	521:523	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	3	44	theme	electron	500:507	arg1	microscopy					509:518	scanning electron microscopy	491:518	scanning electron microscopy (SEM)	491:524	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	8	45	theme	Cd	1470:1471	arg1	levels					1460:1465	toxic levels	1454:1465	toxic levels of Cd	1454:1471	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	3	46	theme	crystallinity	618:630	arg1	%					642:642	~86%	639:642	~86%	639:642	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	3	46	theme	crystallinity	618:630	arg1	index					632:636	high crystallinity index	613:636	high crystallinity index (~86%)	613:643	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	7	47	dep	concentrations	1179:1192	arg1	50 mg L-1					1208:1216	50 mg L-1	1208:1216	50 mg L-1	1208:1216	Moreover, the composite was also found to be competent in removing high concentrations of Cd (25 and 50 mg L-1) from the solution (69-70%).					
32335838	7	47	dep	concentrations	1179:1192	arg1	25					1201:1202	25	1201:1202	25	1201:1202	Moreover, the composite was also found to be competent in removing high concentrations of Cd (25 and 50 mg L-1) from the solution (69-70%).					
32335838	3	48	theme	BNC	481:483	arg1	characterization					457:472	The morpho-structural characterization	435:472	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively	435:574	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	8	49	from	system	1495:1500	arg1	remediation					1439:1449	remediation	1439:1449	remediation of toxic levels of Cd from the contaminated system	1439:1500	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	6	50	attach	removal	1025:1031	arg2	10 mg L-1					1044:1052	initial 10 mg L-1	1036:1052	initial 10 mg L-1 of Cd (II)	1036:1063	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	6	50	attach	removal	1025:1031	arg3	9					1080:1080	9	1080:1080	9	1080:1080	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	6	50	attach	removal	1025:1031	arg3	pH 5					1068:1071	pH 5	1068:1071	pH 5	1068:1071	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	5	51	theme	BNC	853:855	arg1	applicability					827:839	the applicability	823:839	the applicability of produced BNC	823:855	In order to show the applicability of produced BNC, it was modified first using calcite (CaCO3) and thereafter characterized using SEM, XRD, FTIR, and TGA studies.					
32335838	4	52	theme	BNC	707:709	arg1	up					711:712	BNC up	707:712	BNC up to 280 °C	707:722	The thermogravimetric analysis (TGA) showed the stability of BNC up to 280 °C, further rise in temperature to 350 °C results in depolymerization of the sample.					
32335838	6	53	theme	>99	1020:1022	arg1	%					1023:1023	%	1023:1023	%	1023:1023	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	6	54	theme	BNC-CaCO3	974:982	arg1	composites					984:993	The BNC-CaCO3 composites	970:993	The BNC-CaCO3 composites as a sorbent	970:1006	The BNC-CaCO3 composites as a sorbent resulted in >99% removal of initial 10 mg L-1 of Cd (II) at pH 5, 7 and 9 after 12 h of treatment.					
32335838	5	55	theme	produced	844:851	arg1	BNC					853:855	produced BNC	844:855	produced BNC	844:855	In order to show the applicability of produced BNC, it was modified first using calcite (CaCO3) and thereafter characterized using SEM, XRD, FTIR, and TGA studies.					
32335838	7	56	theme	Cd	1197:1198	arg1	concentrations					1179:1192	high concentrations	1174:1192	high concentrations of Cd (25 and 50 mg L-1)	1174:1217	Moreover, the composite was also found to be competent in removing high concentrations of Cd (25 and 50 mg L-1) from the solution (69-70%).					
32335838	1	57	theme	Komagataeibacter	223:238	arg1	SGP8					255:258	Komagataeibacter xylinus strain SGP8	223:258	Komagataeibacter xylinus strain SGP8	223:258	In the present study, fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose is presented.					
32335838	8	58	used	used	1430:1433	arg2	composite					1398:1406	BNC-CaCO3 composite	1388:1406	BNC-CaCO3 composite	1388:1406	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	8	58	used	used	1430:1433	arg2	BNC					1383:1385	modified BNC	1374:1385	modified BNC (BNC-CaCO3 composite)	1374:1407	Overall, the above results suggest that cellulose produced by K. xylinus strain SGP8 showed excellent material properties, and modified BNC (BNC-CaCO3 composite) could effectively be used for remediation of toxic levels of Cd from the contaminated system.					
32335838	3	59	theme	high	613:616	arg1	%					642:642	~86%	639:642	~86%	639:642	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	3	59	theme	high	613:616	arg1	index					632:636	high crystallinity index	613:636	high crystallinity index (~86%)	613:643	The morpho-structural characterization of the BNC using scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies, respectively revealed nanofibrillar structure and high crystallinity index (~86%).					
32335838	1	60	theme	present	142:148	arg1	study					150:154	the present study	138:154	the present study	138:154	In the present study, fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose is presented.					
32335838	1	61	theme	xylinus	240:246	arg1	SGP8					255:258	Komagataeibacter xylinus strain SGP8	223:258	Komagataeibacter xylinus strain SGP8	223:258	In the present study, fermentative production of bacterial nanocellulose (BNC) by using Komagataeibacter xylinus strain SGP8 and characterization of nanocellulose is presented.					
32629438	9	0	theme	In	1641:1642	arg1	studies					1663:1669	In vitro cytotoxicity studies	1641:1669	In vitro cytotoxicity studies	1641:1669	In vitro cytotoxicity studies confirmed the excellent cyto-compatibility of these gels.					
32629438	8	1	theme	3D	1626:1627	arg1	constructs					1629:1638	3D constructs	1626:1638	3D constructs	1626:1638	A model drug quercetin was released in a sustained manner from hydrogels and 3D constructs.					
32629438	10	2	theme	In	1729:1730	arg1	studies					1742:1748	In vivo mice studies	1729:1748	In vivo mice studies	1729:1748	In vivo mice studies prove that these biocompatible hydrogels enhance angiogenesis.					
32629438	9	3	theme	cytotoxicity	1650:1661	arg1	studies					1663:1669	In vitro cytotoxicity studies	1641:1669	In vitro cytotoxicity studies	1641:1669	In vitro cytotoxicity studies confirmed the excellent cyto-compatibility of these gels.					
32629438	7	4	theme	50	1464:1465	arg1	%					1466:1466	%	1466:1466	%	1466:1466	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	4	5	theme	six-membered	738:749	arg1	rings					751:755	the six-membered rings	734:755	the six-membered rings of HA	734:761	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	0	6	theme	Self-crosslinking	0:16	arg1	hydrogel					57:64	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel	0:64	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel	0:64	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel enhances multilayered 3D-printed construct shape integrity and mechanical stability for soft tissue engineering.					
32629438	1	7	theme	3D	228:229	arg1	bioprinting					231:241	extrusion-based 3D bioprinting	212:241	extrusion-based 3D bioprinting	212:241	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	9	8	theme	excellent	1685:1693	arg1	cyto-compatibility					1695:1712	the excellent cyto-compatibility	1681:1712	the excellent cyto-compatibility of these gels	1681:1726	In vitro cytotoxicity studies confirmed the excellent cyto-compatibility of these gels.					
32629438	4	9	theme	hydrolytic	642:651	arg1	stability					653:661	The hydrogel's hydrolytic stability	627:661	The hydrogel's hydrolytic stability	627:661	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	7	10	theme	elastic	1421:1427	arg1	properties					1429:1438	exceptional elastic properties	1409:1438	exceptional elastic properties	1409:1438	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	2	11	theme	post-printing	371:383	arg1	stability					396:404	sufficient post-printing mechanical stability	360:404	sufficient post-printing mechanical stability	360:404	The bioinks should have sufficient post-printing mechanical stability for soft tissue and organ regeneration.					
32629438	9	12	theme	gels	1723:1726	arg1	cyto-compatibility					1695:1712	the excellent cyto-compatibility	1681:1712	the excellent cyto-compatibility of these gels	1681:1726	In vitro cytotoxicity studies confirmed the excellent cyto-compatibility of these gels.					
32629438	11	13	theme	key	1855:1857	arg1	properties					1859:1868	the key properties	1851:1868	the key properties (e.g. self-crosslinking capacity, composition)	1851:1915	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	9	14	dep	In	1641:1642	arg1	vitro					1644:1648	vitro	1644:1648	vitro	1644:1648	In vitro cytotoxicity studies confirmed the excellent cyto-compatibility of these gels.					
32629438	7	15	theme	3D-printed	1374:1383	arg1	constructs					1385:1394	the 3D-printed constructs	1370:1394	the 3D-printed constructs	1370:1394	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	11	16	theme	HA-CMC	1992:1997	arg1	hydrogels					1999:2007	3D-bioprintable HA-CMC hydrogels	1976:2007	3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications	1976:2075	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	5	17	theme	superior	968:975	arg1	precision					977:985	superior precision	968:985	superior precision	968:985	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	6	18	theme	pores	1253:1257	arg1	interconnection					1234:1248	the interconnection	1230:1248	the interconnection of pores	1230:1257	Morphological analyses of different zones of the 3D-printed constructs were undertaken for verification of the interconnection of pores.					
32629438	4	19	theme	bonds	804:808	arg1	stabilization					772:784	the stabilization	768:784	the stabilization of acyl-hydrazone bonds	768:808	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	4	19	theme	bonds	804:808	arg1	prevention					703:712	the prevention	699:712	the prevention of the oxidation of the six-membered rings of HA	699:761	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	10	20	theme	biocompatible	1767:1779	arg1	hydrogels					1781:1789	these biocompatible hydrogels	1761:1789	these biocompatible hydrogels	1761:1789	In vivo mice studies prove that these biocompatible hydrogels enhance angiogenesis.					
32629438	6	21	theme	interconnection	1234:1248	arg1	verification					1214:1225	verification	1214:1225	verification of the interconnection of pores	1214:1257	Morphological analyses of different zones of the 3D-printed constructs were undertaken for verification of the interconnection of pores.					
32629438	0	22	theme	shape	109:113	arg1	integrity					115:123	multilayered 3D-printed construct shape integrity	75:123	multilayered 3D-printed construct shape integrity	75:123	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel enhances multilayered 3D-printed construct shape integrity and mechanical stability for soft tissue engineering.					
32629438	1	23	theme	primary	190:196	arg1	challenges					198:207	the primary challenges	186:207	the primary challenges in extrusion-based 3D bioprinting	186:241	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	7	24	theme	Texture	1260:1266	arg1	TPA					1286:1288	TPA	1286:1288	TPA	1286:1288	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	7	24	theme	Texture	1260:1266	arg1	analysis					1276:1283	Texture profile analysis	1260:1283	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc)	1260:1334	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	1	25	from	challenges	198:207	arg1	bioprinting					231:241	extrusion-based 3D bioprinting	212:241	extrusion-based 3D bioprinting	212:241	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	5	26	theme	layers	956:961	arg1	tube					938:941	tube	938:941	tube	938:941	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	26	theme	layers	956:961	arg1	lattice					919:925	lattice	919:925	lattice	919:925	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	26	theme	layers	956:961	arg1	constructs					907:916	different 3D constructs	894:916	different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7)	894:1120	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	3	27	dep	synthesis	469:477	arg1	the					465:467	the	465:467	the	465:467	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
32629438	0	28	theme	soft	154:157	arg1	engineering					166:176	soft tissue engineering	154:176	soft tissue engineering	154:176	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel enhances multilayered 3D-printed construct shape integrity and mechanical stability for soft tissue engineering.					
32629438	0	29	theme	multilayered	75:86	arg1	integrity					115:123	multilayered 3D-printed construct shape integrity	75:123	multilayered 3D-printed construct shape integrity	75:123	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel enhances multilayered 3D-printed construct shape integrity and mechanical stability for soft tissue engineering.					
32629438	11	30	dep	capacity	1894:1901	arg1	e.g.					1871:1874	e.g.	1871:1874	e.g.	1871:1874	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	7	31	theme	cyclic	1340:1345	arg1	compression					1347:1357	cyclic compression	1340:1357	cyclic compression	1340:1357	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	5	32	theme	self-healing	834:845	arg1	properties					847:856	The shear-thinning and self-healing properties	811:856	The shear-thinning and self-healing properties of the hydrogel	811:872	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	33	dep	50	953:954	arg1	to					950:951	to	950:951	to	950:951	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	3	34	theme	3D	501:502	arg1	printability					504:515	3D printability	501:515	3D printability	501:515	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
32629438	5	35	theme	different	894:902	arg1	tube					938:941	tube	938:941	tube	938:941	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	35	theme	different	894:902	arg1	lattice					919:925	lattice	919:925	lattice	919:925	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	35	theme	different	894:902	arg1	constructs					907:916	different 3D constructs	894:916	different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7)	894:1120	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	36	theme	post-printing	996:1008	arg1	stability					1010:1018	high post-printing stability	991:1018	high post-printing stability	991:1018	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	11	37	theme	constructs	1960:1969	arg1	generation					1933:1942	the generation	1929:1942	the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications	1929:2075	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	0	38	theme	acid-carboxymethylcellulose	29:55	arg1	hydrogel					57:64	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel	0:64	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel	0:64	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel enhances multilayered 3D-printed construct shape integrity and mechanical stability for soft tissue engineering.					
32629438	2	39	contain	have	355:358	arg1	bioinks					340:346	The bioinks	336:346	The bioinks	336:346	The bioinks should have sufficient post-printing mechanical stability for soft tissue and organ regeneration.					
32629438	2	39	contain	have	355:358	arg2	stability					396:404	sufficient post-printing mechanical stability	360:404	sufficient post-printing mechanical stability	360:404	The bioinks should have sufficient post-printing mechanical stability for soft tissue and organ regeneration.					
32629438	1	40	with	print	261:265	arg1	hydrogels					325:333	biocompatible hydrogels	311:333	biocompatible hydrogels	311:333	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	8	41	theme	drug	1557:1560	arg1	quercetin					1562:1570	A model drug quercetin	1549:1570	A model drug quercetin	1549:1570	A model drug quercetin was released in a sustained manner from hydrogels and 3D constructs.					
32629438	3	42	theme	acid	531:534	arg1	hydrogels					570:578	hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels	520:578	hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding	520:624	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
32629438	7	43	theme	CMC	1536:1538	arg1	addition					1524:1531	the addition	1520:1531	the addition of CMC into HA	1520:1546	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	7	44	theme	elastic	1313:1319	arg1	recovery					1321:1328	elastic recovery	1313:1328	elastic recovery	1313:1328	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	7	44	theme	elastic	1313:1319	arg1	hardness					1292:1299	hardness	1292:1299	hardness (strength)	1292:1310	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	3	45	theme	CMC	565:567	arg1	hydrogels					570:578	hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels	520:578	hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding	520:624	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
32629438	0	46	theme	3D-printed	88:97	arg1	integrity					115:123	multilayered 3D-printed construct shape integrity	75:123	multilayered 3D-printed construct shape integrity	75:123	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel enhances multilayered 3D-printed construct shape integrity and mechanical stability for soft tissue engineering.					
32629438	6	47	theme	zones	1159:1163	arg1	analyses					1137:1144	Morphological analyses	1123:1144	Morphological analyses of different zones of the 3D-printed constructs	1123:1192	Morphological analyses of different zones of the 3D-printed constructs were undertaken for verification of the interconnection of pores.					
32629438	3	48	theme	-carboxymethylcellulose	540:562	arg1	hydrogels					570:578	hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels	520:578	hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding	520:624	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
32629438	1	49	theme	self-supported	267:280	arg1	constructs					295:304	self-supported multilayered constructs	267:304	self-supported multilayered constructs	267:304	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	11	50	theme	tissue	2045:2050	arg1	engineering					2052:2062	soft tissue engineering	2040:2062	soft tissue engineering	2040:2062	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	8	51	theme	sustained	1590:1598	arg1	manner					1600:1605	a sustained manner	1588:1605	a sustained manner	1588:1605	A model drug quercetin was released in a sustained manner from hydrogels and 3D constructs.					
32629438	6	52	theme	Morphological	1123:1135	arg1	analyses					1137:1144	Morphological analyses	1123:1144	Morphological analyses of different zones of the 3D-printed constructs	1123:1192	Morphological analyses of different zones of the 3D-printed constructs were undertaken for verification of the interconnection of pores.					
32629438	3	53	theme	hydrogels	570:578	arg1	characterization					480:495	characterization	480:495	characterization	480:495	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
32629438	3	53	theme	hydrogels	570:578	arg1	printability					504:515	3D printability	501:515	3D printability	501:515	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
32629438	3	53	theme	hydrogels	570:578	arg1	synthesis					469:477	synthesis	469:477	synthesis	469:477	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
32629438	5	54	from	post-processing	1049:1063	arg1	compositions					1084:1095	their compositions	1078:1095	their compositions (H7:C3, H5:C5 and H3:C7)	1078:1120	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	1	55	theme	biocompatible	311:323	arg1	hydrogels					325:333	biocompatible hydrogels	311:333	biocompatible hydrogels	311:333	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	5	56	dep	constructs	907:916	arg1	tube					938:941	tube	938:941	tube	938:941	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	56	dep	constructs	907:916	arg1	cubic					928:932	cubic	928:932	cubic	928:932	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	56	dep	constructs	907:916	arg1	lattice					919:925	lattice	919:925	lattice	919:925	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	56	dep	constructs	907:916	arg1	constructs					907:916	different 3D constructs	894:916	different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7)	894:1120	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	2	57	theme	sufficient	360:369	arg1	stability					396:404	sufficient post-printing mechanical stability	360:404	sufficient post-printing mechanical stability	360:404	The bioinks should have sufficient post-printing mechanical stability for soft tissue and organ regeneration.					
32629438	5	58	from	materials	1036:1044	arg1	compositions					1084:1095	their compositions	1078:1095	their compositions (H7:C3, H5:C5 and H3:C7)	1078:1120	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	7	59	theme	%	1466:1466	arg1	strain					1468:1473	50% strain	1464:1473	50% strain	1464:1473	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	2	60	theme	organ	426:430	arg1	regeneration					432:443	organ regeneration	426:443	organ regeneration	426:443	The bioinks should have sufficient post-printing mechanical stability for soft tissue and organ regeneration.					
32629438	11	61	theme	soft	2040:2043	arg1	engineering					2052:2062	soft tissue engineering	2040:2062	soft tissue engineering	2040:2062	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	1	62	theme	extrusion-based	212:226	arg1	bioprinting					231:241	extrusion-based 3D bioprinting	212:241	extrusion-based 3D bioprinting	212:241	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	6	63	theme	constructs	1183:1192	arg1	zones					1159:1163	different zones	1149:1163	different zones of the 3D-printed constructs	1149:1192	Morphological analyses of different zones of the 3D-printed constructs were undertaken for verification of the interconnection of pores.					
32629438	4	64	theme	rings	751:755	arg1	oxidation					721:729	the oxidation	717:729	the oxidation of the six-membered rings of HA	717:761	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	2	65	theme	mechanical	385:394	arg1	stability					396:404	sufficient post-printing mechanical stability	360:404	sufficient post-printing mechanical stability	360:404	The bioinks should have sufficient post-printing mechanical stability for soft tissue and organ regeneration.					
32629438	7	66	theme	fast	1444:1447	arg1	recovery					1449:1456	fast recovery	1444:1456	fast recovery	1444:1456	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	11	67	from	hydrogels	1999:2007	arg1	generation					1933:1942	the generation	1929:1942	the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications	1929:2075	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	5	68	dep	compositions	1084:1095	arg1	H7					1098:1099	H7	1098:1099	H7	1098:1099	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	68	dep	compositions	1084:1095	arg1	H5					1105:1106	H5	1105:1106	H5	1105:1106	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	11	69	theme	self-crosslinking	1876:1892	arg1	composition					1904:1914	composition	1904:1914	composition	1904:1914	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	11	69	theme	self-crosslinking	1876:1892	arg1	capacity					1894:1901	self-crosslinking capacity	1876:1901	self-crosslinking capacity	1876:1901	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	5	70	dep	H7	1098:1099	arg1	H3					1115:1116	H3	1115:1116	H3	1115:1116	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	70	dep	H7	1098:1099	arg1	C5					1108:1109	C5	1108:1109	C5	1108:1109	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	70	dep	H7	1098:1099	arg1	C3					1101:1102	C3	1101:1102	C3	1101:1102	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	70	dep	H7	1098:1099	arg1	C7					1118:1119	C7	1118:1119	C7	1118:1119	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	7	71	theme	exceptional	1409:1419	arg1	properties					1429:1438	exceptional elastic properties	1409:1438	exceptional elastic properties	1409:1438	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	4	72	theme	oxidation	721:729	arg1	stabilization					772:784	the stabilization	768:784	the stabilization of acyl-hydrazone bonds	768:808	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	4	72	theme	oxidation	721:729	arg1	prevention					703:712	the prevention	699:712	the prevention of the oxidation of the six-membered rings of HA	699:761	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	11	73	theme	3D-bioprintable	1976:1990	arg1	hydrogels					1999:2007	3D-bioprintable HA-CMC hydrogels	1976:2007	3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications	1976:2075	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	10	74	theme	mice	1737:1740	arg1	studies					1742:1748	In vivo mice studies	1729:1748	In vivo mice studies	1729:1748	In vivo mice studies prove that these biocompatible hydrogels enhance angiogenesis.					
32629438	7	75	theme	constructs	1385:1394	arg1	studies					1359:1365	studies	1359:1365	studies of the 3D-printed constructs	1359:1394	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	4	76	theme	stabilization	772:784	arg1	effects					683:689	the effects	679:689	the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds	679:808	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	11	77	theme	leveraged	2026:2034	arg1	applications					2064:2075	leveraged for soft tissue engineering applications	2026:2075	leveraged for soft tissue engineering applications	2026:2075	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	6	78	theme	3D-printed	1172:1181	arg1	constructs					1183:1192	the 3D-printed constructs	1168:1192	the 3D-printed constructs	1168:1192	Morphological analyses of different zones of the 3D-printed constructs were undertaken for verification of the interconnection of pores.					
32629438	4	79	theme	acyl-hydrazone	789:802	arg1	bonds					804:808	acyl-hydrazone bonds	789:808	acyl-hydrazone bonds	789:808	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	7	80	theme	profile	1268:1274	arg1	TPA					1286:1288	TPA	1286:1288	TPA	1286:1288	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	7	80	theme	profile	1268:1274	arg1	analysis					1276:1283	Texture profile analysis	1260:1283	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc)	1260:1334	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	8	81	attach	released	1576:1583	arg1	constructs					1629:1638	3D constructs	1626:1638	3D constructs	1626:1638	A model drug quercetin was released in a sustained manner from hydrogels and 3D constructs.					
32629438	8	81	attach	released	1576:1583	arg1	hydrogels					1612:1620	hydrogels	1612:1620	hydrogels	1612:1620	A model drug quercetin was released in a sustained manner from hydrogels and 3D constructs.					
32629438	8	81	attach	released	1576:1583	arg2	quercetin					1562:1570	A model drug quercetin	1549:1570	A model drug quercetin	1549:1570	A model drug quercetin was released in a sustained manner from hydrogels and 3D constructs.					
32629438	4	82	theme	HA	760:761	arg1	rings					751:755	the six-membered rings	734:755	the six-membered rings of HA	734:761	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	0	83	theme	mechanical	129:138	arg1	stability					140:148	mechanical stability	129:148	mechanical stability	129:148	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel enhances multilayered 3D-printed construct shape integrity and mechanical stability for soft tissue engineering.					
32629438	1	84	theme	challenges	198:207	arg1	One					179:181	One	179:181	One	179:181	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	1	84	theme	challenges	198:207	arg1	ability					250:256	the ability to print self-supported multilayered constructs with biocompatible hydrogels	246:333	the ability to print self-supported multilayered constructs with biocompatible hydrogels	246:333	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	1	84	theme	challenges	198:207	arg1	challenges					198:207	the primary challenges	186:207	the primary challenges in extrusion-based 3D bioprinting	186:241	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	5	85	with	layers	956:961	arg1	stability					1010:1018	high post-printing stability	991:1018	high post-printing stability	991:1018	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	85	with	layers	956:961	arg1	precision					977:985	superior precision	968:985	superior precision	968:985	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	86	theme	support	1028:1034	arg1	materials					1036:1044	support materials	1028:1044	support materials	1028:1044	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	10	87	dep	In	1729:1730	arg1	vivo					1732:1735	vivo	1732:1735	vivo	1732:1735	In vivo mice studies prove that these biocompatible hydrogels enhance angiogenesis.					
32629438	0	88	theme	tissue	159:164	arg1	engineering					166:176	soft tissue engineering	154:176	soft tissue engineering	154:176	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel enhances multilayered 3D-printed construct shape integrity and mechanical stability for soft tissue engineering.					
32629438	3	89	theme	hyaluronic	520:529	arg1	HA					537:538	HA	537:538	HA	537:538	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
32629438	3	89	theme	hyaluronic	520:529	arg1	acid					531:534	hyaluronic acid	520:534	hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding	520:624	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
32629438	0	90	theme	hyaluronic	18:27	arg1	hydrogel					57:64	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel	0:64	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel	0:64	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel enhances multilayered 3D-printed construct shape integrity and mechanical stability for soft tissue engineering.					
32629438	11	91	dep	properties	1859:1868	arg1	composition					1904:1914	composition	1904:1914	composition	1904:1914	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	11	91	dep	properties	1859:1868	arg1	capacity					1894:1901	self-crosslinking capacity	1876:1901	self-crosslinking capacity	1876:1901	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	5	92	theme	high	991:994	arg1	stability					1010:1018	high post-printing stability	991:1018	high post-printing stability	991:1018	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	8	93	theme	model	1551:1555	arg1	quercetin					1562:1570	A model drug quercetin	1549:1570	A model drug quercetin	1549:1570	A model drug quercetin was released in a sustained manner from hydrogels and 3D constructs.					
32629438	5	94	theme	3D	904:905	arg1	tube					938:941	tube	938:941	tube	938:941	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	94	theme	3D	904:905	arg1	lattice					919:925	lattice	919:925	lattice	919:925	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	94	theme	3D	904:905	arg1	constructs					907:916	different 3D constructs	894:916	different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7)	894:1120	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	5	95	theme	hydrogel	865:872	arg1	properties					847:856	The shear-thinning and self-healing properties	811:856	The shear-thinning and self-healing properties of the hydrogel	811:872	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	7	96	dep	analysis	1276:1283	arg1	recovery					1321:1328	elastic recovery	1313:1328	elastic recovery	1313:1328	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	7	96	dep	analysis	1276:1283	arg1	strength					1302:1309	strength	1302:1309	strength	1302:1309	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	7	96	dep	analysis	1276:1283	arg1	studies					1359:1365	studies	1359:1365	studies of the 3D-printed constructs	1359:1394	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	7	96	dep	analysis	1276:1283	arg1	hardness					1292:1299	hardness	1292:1299	hardness (strength)	1292:1310	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	7	96	dep	analysis	1276:1283	arg1	etc					1331:1333	etc	1331:1333	etc	1331:1333	Texture profile analysis (TPA) (hardness (strength), elastic recovery, etc) and cyclic compression studies of the 3D-printed constructs demonstrated exceptional elastic properties and fast recovery after 50% strain, respectively, which have been attributed to the addition of CMC into HA.					
32629438	11	97	theme	multilayered	1947:1958	arg1	constructs					1960:1969	multilayered constructs	1947:1969	multilayered constructs	1947:1969	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	0	98	theme	construct	99:107	arg1	integrity					115:123	multilayered 3D-printed construct shape integrity	75:123	multilayered 3D-printed construct shape integrity	75:123	Self-crosslinking hyaluronic acid-carboxymethylcellulose hydrogel enhances multilayered 3D-printed construct shape integrity and mechanical stability for soft tissue engineering.					
32629438	11	99	theme	capable	2009:2015	arg1	hydrogels					1999:2007	3D-bioprintable HA-CMC hydrogels	1976:2007	3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications	1976:2075	The results indicate that controlling the key properties (e.g. self-crosslinking capacity, composition) can lead to the generation of multilayered constructs from 3D-bioprintable HA-CMC hydrogels capable of being leveraged for soft tissue engineering applications.					
32629438	6	100	theme	different	1149:1157	arg1	zones					1159:1163	different zones	1149:1163	different zones of the 3D-printed constructs	1149:1192	Morphological analyses of different zones of the 3D-printed constructs were undertaken for verification of the interconnection of pores.					
32629438	5	101	theme	shear-thinning	815:828	arg1	properties					847:856	The shear-thinning and self-healing properties	811:856	The shear-thinning and self-healing properties of the hydrogel	811:872	The shear-thinning and self-healing properties of the hydrogel allowed us to print different 3D constructs (lattice, cubic and tube) of up to 50 layers with superior precision and high post-printing stability without support materials or post-processing depending on their compositions (H7:C3, H5:C5 and H3:C7).					
32629438	2	102	theme	soft	410:413	arg1	tissue					415:420	soft tissue	410:420	soft tissue	410:420	The bioinks should have sufficient post-printing mechanical stability for soft tissue and organ regeneration.					
32629438	1	103	theme	multilayered	282:293	arg1	constructs					295:304	self-supported multilayered constructs	267:304	self-supported multilayered constructs	267:304	One of the primary challenges in extrusion-based 3D bioprinting is the ability to print self-supported multilayered constructs with biocompatible hydrogels.					
32629438	4	104	theme	prevention	703:712	arg1	effects					683:689	the effects	679:689	the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds	679:808	The hydrogel's hydrolytic stability was acquired by the effects of both the prevention of the oxidation of the six-membered rings of HA, and the stabilization of acyl-hydrazone bonds.					
32629438	3	105	theme	N-acyl-hydrazone	601:616	arg1	bonding					618:624	N-acyl-hydrazone bonding	601:624	N-acyl-hydrazone bonding	601:624	Here, we report on the synthesis, characterization and 3D printability of hyaluronic acid (HA)-carboxymethylcellulose (CMC) hydrogels cross-linked through N-acyl-hydrazone bonding.					
34384800	1	0	theme	liquor	170:175	arg1	by-products					177:187	yeast-fermented Korean liquor by-products	147:187	yeast-fermented Korean liquor by-products	147:187	To increase the value of yeast-fermented Korean liquor by-products, we obtained crude polysaccharide (CPS) fractions via ultrasound-assisted extraction and stepwise-gradient ethanol precipitation and investigated their functionality.					
34384800	4	1	with	polysaccharides	789:803	arg1	<15 kDa					835:841	<15 kDa	835:841	<15 kDa	835:841	In addition, a higher concentration of ethanol solvent for fractionation yielded polysaccharides with lower molecular weights (<15 kDa).					
34384800	4	1	with	polysaccharides	789:803	arg1	weights					826:832	lower molecular weights	810:832	lower molecular weights (<15 kDa)	810:842	In addition, a higher concentration of ethanol solvent for fractionation yielded polysaccharides with lower molecular weights (<15 kDa).					
34384800	1	2	theme	by-products	177:187	arg1	value					138:142	the value	134:142	the value of yeast-fermented Korean liquor by-products	134:187	To increase the value of yeast-fermented Korean liquor by-products, we obtained crude polysaccharide (CPS) fractions via ultrasound-assisted extraction and stepwise-gradient ethanol precipitation and investigated their functionality.					
34384800	2	3	from	production	369:378	arg1	cells					393:397	RAW 264.7 cells	383:397	RAW 264.7 cells	383:397	Nitric oxide production in RAW 264.7 cells was increased following treatment with the CPSs derived from extract.					
34384800	1	4	theme	stepwise-gradient	278:294	arg1	precipitation					304:316	stepwise-gradient ethanol precipitation	278:316	stepwise-gradient ethanol precipitation	278:316	To increase the value of yeast-fermented Korean liquor by-products, we obtained crude polysaccharide (CPS) fractions via ultrasound-assisted extraction and stepwise-gradient ethanol precipitation and investigated their functionality.					
34384800	5	5	theme	non-treated	1075:1085	arg1	cells					1097:1101	non-treated RAW 264.7 cells	1075:1101	non-treated RAW 264.7 cells	1075:1101	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	1	6	theme	ethanol	296:302	arg1	precipitation					304:316	stepwise-gradient ethanol precipitation	278:316	stepwise-gradient ethanol precipitation	278:316	To increase the value of yeast-fermented Korean liquor by-products, we obtained crude polysaccharide (CPS) fractions via ultrasound-assisted extraction and stepwise-gradient ethanol precipitation and investigated their functionality.					
34384800	0	7	theme	Saccharomyces	96:108	arg1	cerevisiae					110:119	Saccharomyces cerevisiae	96:119	Saccharomyces cerevisiae	96:119	Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor fermented by Saccharomyces cerevisiae.					
34384800	8	8	theme	extraction	1448:1457	arg1	techniques					1459:1468	eco-friendly extraction techniques	1435:1468	eco-friendly extraction techniques	1435:1468	Therefore, this research can contribute to the development of eco-friendly extraction techniques and immune-enhancing materials.					
34384800	7	9	theme	immune-enhancing	1347:1362	arg1	effects					1364:1370	its immune-enhancing effects	1343:1370	its immune-enhancing effects	1343:1370	These results indicated that CPS 4 enhanced the proliferation of mouse spleen cells in vivo, indicating its immune-enhancing effects.					
34384800	5	10	theme	CPS	845:847	arg1	fractions					857:865	CPS 3 and 4 fractions	845:865	CPS 3 and 4 fractions	845:865	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	5	11	theme	concentration	1026:1038	arg1	12.5 μg/mL					1040:1049	concentration 12.5 μg/mL	1026:1049	concentration 12.5 μg/mL	1026:1049	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	6	12	theme	cells	1201:1205	arg1	proliferation					1171:1183	the proliferation	1167:1183	the proliferation of mouse spleen cells	1167:1205	Especially, CPS 4 at 200 and 400 μg/mL significantly increased the proliferation of mouse spleen cells by 126% and 153%, respectively.					
34384800	4	13	theme	solvent	755:761	arg1	ethanol					747:753	ethanol solvent	747:761	ethanol solvent	747:761	In addition, a higher concentration of ethanol solvent for fractionation yielded polysaccharides with lower molecular weights (<15 kDa).					
34384800	3	14	theme	monosaccharide	485:498	arg1	Analysis					469:476	Analysis	469:476	Analysis of the monosaccharide and amino acid composition of the CPS fractions using HPLC	469:557	Analysis of the monosaccharide and amino acid composition of the CPS fractions using HPLC revealed that the polysaccharides were mainly composed of glucose (57.2%), mannose (22.6%), and galactose (17.6%), and no amino acids were detected.					
34384800	5	15	from	levels	1065:1070	arg1	cells					1097:1101	non-treated RAW 264.7 cells	1075:1101	non-treated RAW 264.7 cells	1075:1101	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	6	16	theme	spleen	1194:1199	arg1	cells					1201:1205	mouse spleen cells	1188:1205	mouse spleen cells	1188:1205	Especially, CPS 4 at 200 and 400 μg/mL significantly increased the proliferation of mouse spleen cells by 126% and 153%, respectively.					
34384800	4	17	theme	ethanol	747:753	arg1	concentration					730:742	a higher concentration	721:742	a higher concentration of ethanol solvent for fractionation	721:779	In addition, a higher concentration of ethanol solvent for fractionation yielded polysaccharides with lower molecular weights (<15 kDa).					
34384800	5	18	theme	3	849:849	arg1	fractions					857:865	CPS 3 and 4 fractions	845:865	CPS 3 and 4 fractions	845:865	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	8	19	theme	techniques	1459:1468	arg1	development					1420:1430	the development	1416:1430	the development of eco-friendly extraction techniques and immune-enhancing materials	1416:1499	Therefore, this research can contribute to the development of eco-friendly extraction techniques and immune-enhancing materials.					
34384800	6	20	theme	mouse	1188:1192	arg1	cells					1201:1205	mouse spleen cells	1188:1205	mouse spleen cells	1188:1205	Especially, CPS 4 at 200 and 400 μg/mL significantly increased the proliferation of mouse spleen cells by 126% and 153%, respectively.					
34384800	8	21	theme	eco-friendly	1435:1446	arg1	techniques					1459:1468	eco-friendly extraction techniques	1435:1468	eco-friendly extraction techniques	1435:1468	Therefore, this research can contribute to the development of eco-friendly extraction techniques and immune-enhancing materials.					
34384800	0	22	theme	Immune-enhancing	0:15	arg1	effects					17:23	Immune-enhancing effects	0:23	Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor	0:81	Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor fermented by Saccharomyces cerevisiae.					
34384800	8	23	theme	materials	1491:1499	arg1	development					1420:1430	the development	1416:1430	the development of eco-friendly extraction techniques and immune-enhancing materials	1416:1499	Therefore, this research can contribute to the development of eco-friendly extraction techniques and immune-enhancing materials.					
34384800	2	24	theme	oxide	363:367	arg1	production					369:378	Nitric oxide production	356:378	Nitric oxide production in RAW 264.7 cells	356:397	Nitric oxide production in RAW 264.7 cells was increased following treatment with the CPSs derived from extract.					
34384800	1	25	theme	crude	202:206	arg1	fractions					229:237	crude polysaccharide (CPS) fractions	202:237	crude polysaccharide (CPS) fractions	202:237	To increase the value of yeast-fermented Korean liquor by-products, we obtained crude polysaccharide (CPS) fractions via ultrasound-assisted extraction and stepwise-gradient ethanol precipitation and investigated their functionality.					
34384800	0	26	theme	polysaccharide	28:41	arg1	extract					43:49	polysaccharide extract	28:49	polysaccharide extract	28:49	Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor fermented by Saccharomyces cerevisiae.					
34384800	8	27	theme	immune-enhancing	1474:1489	arg1	materials					1491:1499	immune-enhancing materials	1474:1499	immune-enhancing materials	1474:1499	Therefore, this research can contribute to the development of eco-friendly extraction techniques and immune-enhancing materials.					
34384800	5	28	theme	iNOS	976:979	arg1	expression					1012:1021	IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression	936:1021	expression	1012:1021	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	2	29	theme	Nitric	356:361	arg1	production					369:378	Nitric oxide production	356:378	Nitric oxide production in RAW 264.7 cells	356:397	Nitric oxide production in RAW 264.7 cells was increased following treatment with the CPSs derived from extract.					
34384800	1	30	theme	polysaccharide	208:221	arg1	fractions					229:237	crude polysaccharide (CPS) fractions	202:237	crude polysaccharide (CPS) fractions	202:237	To increase the value of yeast-fermented Korean liquor by-products, we obtained crude polysaccharide (CPS) fractions via ultrasound-assisted extraction and stepwise-gradient ethanol precipitation and investigated their functionality.					
34384800	7	31	theme	cells	1317:1321	arg1	proliferation					1287:1299	the proliferation	1283:1299	the proliferation of mouse spleen cells	1283:1321	These results indicated that CPS 4 enhanced the proliferation of mouse spleen cells in vivo, indicating its immune-enhancing effects.					
34384800	3	32	theme	amino	504:508	arg1	composition					515:525	the monosaccharide and amino acid composition	481:525	composition	515:525	Analysis of the monosaccharide and amino acid composition of the CPS fractions using HPLC revealed that the polysaccharides were mainly composed of glucose (57.2%), mannose (22.6%), and galactose (17.6%), and no amino acids were detected.					
34384800	4	33	theme	lower	810:814	arg1	<15 kDa					835:841	<15 kDa	835:841	<15 kDa	835:841	In addition, a higher concentration of ethanol solvent for fractionation yielded polysaccharides with lower molecular weights (<15 kDa).					
34384800	4	33	theme	lower	810:814	arg1	weights					826:832	lower molecular weights	810:832	lower molecular weights (<15 kDa)	810:842	In addition, a higher concentration of ethanol solvent for fractionation yielded polysaccharides with lower molecular weights (<15 kDa).					
34384800	5	34	theme	4	855:855	arg1	fractions					857:865	CPS 3 and 4 fractions	845:865	CPS 3 and 4 fractions	845:865	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	1	35	theme	CPS	224:226	arg1	fractions					229:237	crude polysaccharide (CPS) fractions	202:237	crude polysaccharide (CPS) fractions	202:237	To increase the value of yeast-fermented Korean liquor by-products, we obtained crude polysaccharide (CPS) fractions via ultrasound-assisted extraction and stepwise-gradient ethanol precipitation and investigated their functionality.					
34384800	0	36	theme	extract	43:49	arg1	effects					17:23	Immune-enhancing effects	0:23	Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor	0:81	Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor fermented by Saccharomyces cerevisiae.					
34384800	5	37	theme	RAW	1087:1089	arg1	cells					1097:1101	non-treated RAW 264.7 cells	1075:1101	non-treated RAW 264.7 cells	1075:1101	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	7	38	theme	spleen	1310:1315	arg1	cells					1317:1321	mouse spleen cells	1304:1321	mouse spleen cells	1304:1321	These results indicated that CPS 4 enhanced the proliferation of mouse spleen cells in vivo, indicating its immune-enhancing effects.					
34384800	7	39	theme	mouse	1304:1308	arg1	cells					1317:1321	mouse spleen cells	1304:1321	mouse spleen cells	1304:1321	These results indicated that CPS 4 enhanced the proliferation of mouse spleen cells in vivo, indicating its immune-enhancing effects.					
34384800	3	40	theme	acid	510:513	arg1	composition					515:525	the monosaccharide and amino acid composition	481:525	composition	515:525	Analysis of the monosaccharide and amino acid composition of the CPS fractions using HPLC revealed that the polysaccharides were mainly composed of glucose (57.2%), mannose (22.6%), and galactose (17.6%), and no amino acids were detected.					
34384800	3	41	theme	fractions	538:546	arg1	monosaccharide					485:498	the monosaccharide and amino acid composition	481:525	monosaccharide	485:498	Analysis of the monosaccharide and amino acid composition of the CPS fractions using HPLC revealed that the polysaccharides were mainly composed of glucose (57.2%), mannose (22.6%), and galactose (17.6%), and no amino acids were detected.					
34384800	3	41	theme	fractions	538:546	arg1	composition					515:525	the monosaccharide and amino acid composition	481:525	composition	515:525	Analysis of the monosaccharide and amino acid composition of the CPS fractions using HPLC revealed that the polysaccharides were mainly composed of glucose (57.2%), mannose (22.6%), and galactose (17.6%), and no amino acids were detected.					
34384800	0	42	theme	by-products	54:64	arg1	effects					17:23	Immune-enhancing effects	0:23	Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor	0:81	Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor fermented by Saccharomyces cerevisiae.					
34384800	4	43	theme	molecular	816:824	arg1	<15 kDa					835:841	<15 kDa	835:841	<15 kDa	835:841	In addition, a higher concentration of ethanol solvent for fractionation yielded polysaccharides with lower molecular weights (<15 kDa).					
34384800	4	43	theme	molecular	816:824	arg1	weights					826:832	lower molecular weights	810:832	lower molecular weights (<15 kDa)	810:842	In addition, a higher concentration of ethanol solvent for fractionation yielded polysaccharides with lower molecular weights (<15 kDa).					
34384800	5	44	dep	IL-6	936:939	arg1	20					942:943	20	942:943	20	942:943	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	5	44	dep	IL-6	936:939	arg1	respectively					958:969	respectively	958:969	respectively	958:969	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	5	45	theme	TNF-α	895:899	arg1	production					881:890	the production	877:890	the production of TNF-α (15 and 17-fold, respectively)	877:930	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	5	45	theme	TNF-α	895:899	arg1	IL-6					936:939	IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression	936:1021	IL-6	936:939	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	5	45	theme	TNF-α	895:899	arg1	expression					1012:1021	IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression	936:1021	expression	1012:1021	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	2	46	attach	derived	447:453	arg1	extract					460:466	extract	460:466	extract	460:466	Nitric oxide production in RAW 264.7 cells was increased following treatment with the CPSs derived from extract.					
34384800	2	46	attach	derived	447:453	arg2	CPSs					442:445	the CPSs	438:445	the CPSs derived from extract	438:466	Nitric oxide production in RAW 264.7 cells was increased following treatment with the CPSs derived from extract.					
34384800	2	47	theme	RAW	383:385	arg1	cells					393:397	RAW 264.7 cells	383:397	RAW 264.7 cells	383:397	Nitric oxide production in RAW 264.7 cells was increased following treatment with the CPSs derived from extract.					
34384800	4	48	theme	higher	723:728	arg1	concentration					730:742	a higher concentration	721:742	a higher concentration of ethanol solvent for fractionation	721:779	In addition, a higher concentration of ethanol solvent for fractionation yielded polysaccharides with lower molecular weights (<15 kDa).					
34384800	5	49	dep	TNF-α	895:899	arg1	respectively					918:929	respectively	918:929	respectively	918:929	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	5	49	dep	TNF-α	895:899	arg1	15					902:903	15	902:903	15	902:903	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	2	50	with	treatment	423:431	arg1	CPSs					442:445	the CPSs	438:445	the CPSs derived from extract	438:466	Nitric oxide production in RAW 264.7 cells was increased following treatment with the CPSs derived from extract.					
34384800	3	51	theme	CPS	534:536	arg1	fractions					538:546	the CPS fractions	530:546	the CPS fractions	530:546	Analysis of the monosaccharide and amino acid composition of the CPS fractions using HPLC revealed that the polysaccharides were mainly composed of glucose (57.2%), mannose (22.6%), and galactose (17.6%), and no amino acids were detected.					
34384800	5	52	dep	expression	1012:1021	arg1	35-fold					989:995	35-fold	989:995	35-fold	989:995	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	5	52	dep	expression	1012:1021	arg1	65					982:983	65	982:983	65	982:983	CPS 3 and 4 fractions increased the production of TNF-α (15 and 17-fold, respectively) and IL-6 (20 and 18-fold, respectively) and iNOS (65 and 35-fold, respectively) expression at concentration 12.5 μg/mL compared with levels in non-treated RAW 264.7 cells.					
34384800	0	53	theme	liquor	76:81	arg1	by-products					54:64	by-products	54:64	by-products of Korean liquor	54:81	Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor fermented by Saccharomyces cerevisiae.					
34384800	3	54	theme	composition	515:525	arg1	Analysis					469:476	Analysis	469:476	Analysis of the monosaccharide and amino acid composition of the CPS fractions using HPLC	469:557	Analysis of the monosaccharide and amino acid composition of the CPS fractions using HPLC revealed that the polysaccharides were mainly composed of glucose (57.2%), mannose (22.6%), and galactose (17.6%), and no amino acids were detected.					
34384800	1	55	theme	yeast-fermented	147:161	arg1	by-products					177:187	yeast-fermented Korean liquor by-products	147:187	yeast-fermented Korean liquor by-products	147:187	To increase the value of yeast-fermented Korean liquor by-products, we obtained crude polysaccharide (CPS) fractions via ultrasound-assisted extraction and stepwise-gradient ethanol precipitation and investigated their functionality.					
34384800	1	56	theme	ultrasound-assisted	243:261	arg1	extraction					263:272	ultrasound-assisted extraction	243:272	ultrasound-assisted extraction	243:272	To increase the value of yeast-fermented Korean liquor by-products, we obtained crude polysaccharide (CPS) fractions via ultrasound-assisted extraction and stepwise-gradient ethanol precipitation and investigated their functionality.					
34384800	0	57	theme	Korean	69:74	arg1	liquor					76:81	Korean liquor	69:81	Korean liquor	69:81	Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor fermented by Saccharomyces cerevisiae.					
34384800	3	58	theme	amino	681:685	arg1	acids					687:691	no amino acids	678:691	no amino acids	678:691	Analysis of the monosaccharide and amino acid composition of the CPS fractions using HPLC revealed that the polysaccharides were mainly composed of glucose (57.2%), mannose (22.6%), and galactose (17.6%), and no amino acids were detected.					
34384800	1	59	theme	Korean	163:168	arg1	by-products					177:187	yeast-fermented Korean liquor by-products	147:187	yeast-fermented Korean liquor by-products	147:187	To increase the value of yeast-fermented Korean liquor by-products, we obtained crude polysaccharide (CPS) fractions via ultrasound-assisted extraction and stepwise-gradient ethanol precipitation and investigated their functionality.					
32363671	3	0	theme	mechanical	331:340	arg1	property					342:349	mechanical property	331:349	mechanical property	331:349	Although polysaccharide modification can improve the mechanical property and biological activity of gelatin films, information about the stability and cellular affinity is still limited.					
32363671	8	1	theme	jelly-derived	1037:1049	arg1	cells					1068:1072	jelly-derived mesenchymal stem cells	1037:1072	human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface	1006:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	1	theme	jelly-derived	1037:1049	arg1	MSCs					1078:1081	WJ MSCs	1075:1081	WJ MSCs	1075:1081	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	2	theme	umbilical	1012:1020	arg1	Wharton					1027:1033	human umbilical cord Wharton	1006:1033	human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface	1006:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	7	3	theme	G-CS	913:916	arg1	film					918:921	G-CS film	913:921	G-CS film	913:921	And G-HA composite film also had better biological safety than G-CS film.					
32363671	0	4	theme	films	81:85	arg1	stability					13:21	stability	13:21	stability	13:21	Study on the stability and cellular affinity of gelatin-polysaccharide composite films.					
32363671	0	4	theme	films	81:85	arg1	affinity					36:43	cellular affinity	27:43	cellular affinity	27:43	Study on the stability and cellular affinity of gelatin-polysaccharide composite films.					
32363671	4	5	theme	cellular	543:550	arg1	affinity					552:559	cellular affinity	543:559	cellular affinity	543:559	This study investigated the influence of polysaccharides on the stability and cellular affinity of gelatin films.					
32363671	9	6	theme	G-CS	1220:1223	arg1	film					1225:1228	G-CS film	1220:1228	G-CS film	1220:1228	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	9	7	theme	composite	1148:1156	arg1	film					1158:1161	G-HA composite film	1143:1161	G-HA composite film	1143:1161	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	10	8	theme	composite	1425:1433	arg1	films					1435:1439	gelatin-polysaccharide composite films	1402:1439	gelatin-polysaccharide composite films	1402:1439	Therefore, this work would be very helpful to optimize the preparation of gelatin-polysaccharide composite films.					
32363671	7	9	theme	biological	890:899	arg1	safety					901:906	better biological safety	883:906	better biological safety	883:906	And G-HA composite film also had better biological safety than G-CS film.					
32363671	10	10	theme	gelatin-polysaccharide	1402:1423	arg1	films					1435:1439	gelatin-polysaccharide composite films	1402:1439	gelatin-polysaccharide composite films	1402:1439	Therefore, this work would be very helpful to optimize the preparation of gelatin-polysaccharide composite films.					
32363671	3	11	theme	gelatin	378:384	arg1	films					386:390	gelatin films	378:390	gelatin films	378:390	Although polysaccharide modification can improve the mechanical property and biological activity of gelatin films, information about the stability and cellular affinity is still limited.					
32363671	8	12	theme	human	1006:1010	arg1	Wharton					1027:1033	human umbilical cord Wharton	1006:1033	human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface	1006:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	13	theme	stem	1063:1066	arg1	cells					1068:1072	jelly-derived mesenchymal stem cells	1037:1072	human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface	1006:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	13	theme	stem	1063:1066	arg1	MSCs					1078:1081	WJ MSCs	1075:1081	WJ MSCs	1075:1081	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	5	14	theme	gelatin-hyaluronic	642:659	arg1	G-HA					667:670	G-HA	667:670	G-HA	667:670	Two kinds of gelatin-polysaccharide composite films, including gelatin-hyaluronic acid (G-HA) and gelatin-chitosan (G-CS), were prepared in this study.					
32363671	5	14	theme	gelatin-hyaluronic	642:659	arg1	acid					661:664	gelatin-hyaluronic acid	642:664	gelatin-hyaluronic acid (G-HA)	642:671	Two kinds of gelatin-polysaccharide composite films, including gelatin-hyaluronic acid (G-HA) and gelatin-chitosan (G-CS), were prepared in this study.					
32363671	9	15	theme	better	1167:1172	arg1	stability					1174:1182	better stability	1167:1182	better stability	1167:1182	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	3	16	theme	films	386:390	arg1	property					342:349	mechanical property	331:349	mechanical property	331:349	Although polysaccharide modification can improve the mechanical property and biological activity of gelatin films, information about the stability and cellular affinity is still limited.					
32363671	3	16	theme	films	386:390	arg1	activity					366:373	biological activity	355:373	biological activity	355:373	Although polysaccharide modification can improve the mechanical property and biological activity of gelatin films, information about the stability and cellular affinity is still limited.					
32363671	8	17	theme	mesenchymal	1051:1061	arg1	cells					1068:1072	jelly-derived mesenchymal stem cells	1037:1072	human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface	1006:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	17	theme	mesenchymal	1051:1061	arg1	MSCs					1078:1081	WJ MSCs	1075:1081	WJ MSCs	1075:1081	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	6	18	theme	G-CS	829:832	arg1	film					844:847	G-CS composite film	829:847	G-CS composite film	829:847	It was found that G-HA composite film had better short-term and long-term stability compared with G-CS composite film.					
32363671	8	19	theme	film	964:967	arg1	surface					938:944	the surface	934:944	the surface of G-HA composite film	934:967	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	20	dep	Wharton	1027:1033	arg1	cells					1068:1072	jelly-derived mesenchymal stem cells	1037:1072	human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface	1006:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	20	dep	Wharton	1027:1033	arg1	MSCs					1078:1081	WJ MSCs	1075:1081	WJ MSCs	1075:1081	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	9	21	theme	further	1303:1309	arg1	studies					1319:1325	further in vivo studies	1303:1325	further in vivo studies	1303:1325	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	9	22	theme	G-HA	1143:1146	arg1	film					1158:1161	G-HA composite film	1143:1161	G-HA composite film	1143:1161	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	7	23	theme	better	883:888	arg1	safety					901:906	better biological safety	883:906	better biological safety	883:906	And G-HA composite film also had better biological safety than G-CS film.					
32363671	1	24	theme	wound	167:171	arg1	healing					173:179	wound healing	167:179	wound healing	167:179	The gelatin film has great potential in biomedical applications, especially in wound healing.					
32363671	9	25	theme	in	1311:1312	arg1	studies					1319:1325	further in vivo studies	1303:1325	further in vivo studies	1303:1325	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	0	26	theme	cellular	27:34	arg1	affinity					36:43	cellular affinity	27:43	cellular affinity	27:43	Study on the stability and cellular affinity of gelatin-polysaccharide composite films.					
32363671	5	27	theme	gelatin-polysaccharide	592:613	arg1	films					625:629	gelatin-polysaccharide composite films	592:629	gelatin-polysaccharide composite films	592:629	Two kinds of gelatin-polysaccharide composite films, including gelatin-hyaluronic acid (G-HA) and gelatin-chitosan (G-CS), were prepared in this study.					
32363671	4	28	dep	stability	529:537	arg1	the					525:527	the	525:527	the	525:527	This study investigated the influence of polysaccharides on the stability and cellular affinity of gelatin films.					
32363671	4	29	theme	polysaccharides	506:520	arg1	influence					493:501	the influence	489:501	the influence of polysaccharides on the stability and cellular affinity of gelatin films	489:576	This study investigated the influence of polysaccharides on the stability and cellular affinity of gelatin films.					
32363671	5	30	theme	composite	615:623	arg1	films					625:629	gelatin-polysaccharide composite films	592:629	gelatin-polysaccharide composite films	592:629	Two kinds of gelatin-polysaccharide composite films, including gelatin-hyaluronic acid (G-HA) and gelatin-chitosan (G-CS), were prepared in this study.					
32363671	6	31	contain	had	769:771	arg1	film					764:767	G-HA composite film	749:767	G-HA composite film	749:767	It was found that G-HA composite film had better short-term and long-term stability compared with G-CS composite film.					
32363671	6	31	contain	had	769:771	arg2	stability					805:813	better short-term and long-term stability	773:813	better short-term and long-term stability	773:813	It was found that G-HA composite film had better short-term and long-term stability compared with G-CS composite film.					
32363671	4	32	from	influence	493:501	arg1	stability					529:537	stability	529:537	stability	529:537	This study investigated the influence of polysaccharides on the stability and cellular affinity of gelatin films.					
32363671	4	32	from	influence	493:501	arg1	affinity					552:559	cellular affinity	543:559	cellular affinity	543:559	This study investigated the influence of polysaccharides on the stability and cellular affinity of gelatin films.					
32363671	5	33	theme	films	625:629	arg1	acid					661:664	gelatin-hyaluronic acid	642:664	gelatin-hyaluronic acid (G-HA)	642:671	Two kinds of gelatin-polysaccharide composite films, including gelatin-hyaluronic acid (G-HA) and gelatin-chitosan (G-CS), were prepared in this study.					
32363671	5	33	theme	films	625:629	arg1	gelatin-chitosan					677:692	gelatin-chitosan	677:692	gelatin-chitosan (G-CS)	677:699	Two kinds of gelatin-polysaccharide composite films, including gelatin-hyaluronic acid (G-HA) and gelatin-chitosan (G-CS), were prepared in this study.					
32363671	5	33	theme	films	625:629	arg1	kinds					583:587	Two kinds	579:587	Two kinds	579:587	Two kinds of gelatin-polysaccharide composite films, including gelatin-hyaluronic acid (G-HA) and gelatin-chitosan (G-CS), were prepared in this study.					
32363671	1	34	theme	gelatin	92:98	arg1	film					100:103	The gelatin film	88:103	The gelatin film	88:103	The gelatin film has great potential in biomedical applications, especially in wound healing.					
32363671	7	35	theme	G-HA	854:857	arg1	film					869:872	G-HA composite film	854:872	G-HA composite film	854:872	And G-HA composite film also had better biological safety than G-CS film.					
32363671	8	36	theme	Wharton	1027:1033	arg1	growth					996:1001	growth	996:1001	growth	996:1001	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	36	theme	Wharton	1027:1033	arg1	adhesion					983:990	adhesion	983:990	adhesion	983:990	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	6	37	theme	composite	754:762	arg1	film					764:767	G-HA composite film	749:767	G-HA composite film	749:767	It was found that G-HA composite film had better short-term and long-term stability compared with G-CS composite film.					
32363671	8	38	theme	better	1084:1089	arg1	Wharton					1027:1033	human umbilical cord Wharton	1006:1033	human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface	1006:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	39	theme	cord	1022:1025	arg1	Wharton					1027:1033	human umbilical cord Wharton	1006:1033	human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface	1006:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	7	40	theme	composite	859:867	arg1	film					869:872	G-HA composite film	854:872	G-HA composite film	854:872	And G-HA composite film also had better biological safety than G-CS film.					
32363671	6	41	theme	long-term	795:803	arg1	stability					805:813	better short-term and long-term stability	773:813	better short-term and long-term stability	773:813	It was found that G-HA composite film had better short-term and long-term stability compared with G-CS composite film.					
32363671	3	42	dep	property	342:349	arg1	the					327:329	the	327:329	the	327:329	Although polysaccharide modification can improve the mechanical property and biological activity of gelatin films, information about the stability and cellular affinity is still limited.					
32363671	3	43	theme	cellular	429:436	arg1	affinity					438:445	cellular affinity	429:445	cellular affinity	429:445	Although polysaccharide modification can improve the mechanical property and biological activity of gelatin films, information about the stability and cellular affinity is still limited.					
32363671	8	44	theme	G-CS	1096:1099	arg1	surface					1106:1112	G-CS film surface	1096:1112	G-CS film surface	1096:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	45	theme	composite	954:962	arg1	film					964:967	G-HA composite film	949:967	G-HA composite film	949:967	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	1	46	contain	has	105:107	arg1	film					100:103	The gelatin film	88:103	The gelatin film	88:103	The gelatin film has great potential in biomedical applications, especially in wound healing.					
32363671	1	46	contain	has	105:107	arg2	potential					115:123	great potential	109:123	great potential	109:123	The gelatin film has great potential in biomedical applications, especially in wound healing.					
32363671	2	47	theme	cells	224:228	arg1	combination					186:196	The combination	182:196	The combination of gelatin films and stem cells	182:228	The combination of gelatin films and stem cells could further accelerate the skin regeneration.					
32363671	6	48	theme	short-term	780:789	arg1	stability					805:813	better short-term and long-term stability	773:813	better short-term and long-term stability	773:813	It was found that G-HA composite film had better short-term and long-term stability compared with G-CS composite film.					
32363671	9	49	contain	has	1163:1165	arg1	film					1158:1161	G-HA composite film	1143:1161	G-HA composite film	1143:1161	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	9	49	contain	has	1163:1165	arg2	affinity					1197:1204	cellular affinity	1188:1204	cellular affinity	1188:1204	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	9	49	contain	has	1163:1165	arg2	stability					1174:1182	better stability	1167:1182	better stability	1167:1182	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	9	50	theme	cellular	1188:1195	arg1	affinity					1197:1204	cellular affinity	1188:1204	cellular affinity	1188:1204	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	0	51	theme	gelatin-polysaccharide	48:69	arg1	films					81:85	gelatin-polysaccharide composite films	48:85	gelatin-polysaccharide composite films	48:85	Study on the stability and cellular affinity of gelatin-polysaccharide composite films.					
32363671	2	52	theme	stem	219:222	arg1	cells					224:228	stem cells	219:228	stem cells	219:228	The combination of gelatin films and stem cells could further accelerate the skin regeneration.					
32363671	1	53	theme	great	109:113	arg1	potential					115:123	great potential	109:123	great potential	109:123	The gelatin film has great potential in biomedical applications, especially in wound healing.					
32363671	9	54	theme	cells	1293:1297	arg1	system					1278:1283	a promising delivery system	1257:1283	a promising delivery system of stem cells for further in vivo studies	1257:1325	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	4	55	theme	films	572:576	arg1	stability					529:537	stability	529:537	stability	529:537	This study investigated the influence of polysaccharides on the stability and cellular affinity of gelatin films.					
32363671	4	55	theme	films	572:576	arg1	affinity					552:559	cellular affinity	543:559	cellular affinity	543:559	This study investigated the influence of polysaccharides on the stability and cellular affinity of gelatin films.					
32363671	8	56	theme	film	1101:1104	arg1	surface					1106:1112	G-CS film surface	1096:1112	G-CS film surface	1096:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	2	57	theme	films	209:213	arg1	combination					186:196	The combination	182:196	The combination of gelatin films and stem cells	182:228	The combination of gelatin films and stem cells could further accelerate the skin regeneration.					
32363671	2	58	theme	gelatin	201:207	arg1	films					209:213	gelatin films	201:213	gelatin films	201:213	The combination of gelatin films and stem cells could further accelerate the skin regeneration.					
32363671	8	59	theme	G-HA	949:952	arg1	film					964:967	G-HA composite film	949:967	G-HA composite film	949:967	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	10	60	theme	films	1435:1439	arg1	preparation					1387:1397	the preparation	1383:1397	the preparation of gelatin-polysaccharide composite films	1383:1439	Therefore, this work would be very helpful to optimize the preparation of gelatin-polysaccharide composite films.					
32363671	4	61	theme	gelatin	564:570	arg1	films					572:576	gelatin films	564:576	gelatin films	564:576	This study investigated the influence of polysaccharides on the stability and cellular affinity of gelatin films.					
32363671	0	62	dep	stability	13:21	arg1	the					9:11	the	9:11	the	9:11	Study on the stability and cellular affinity of gelatin-polysaccharide composite films.					
32363671	2	63	theme	skin	259:262	arg1	regeneration					264:275	the skin regeneration	255:275	the skin regeneration	255:275	The combination of gelatin films and stem cells could further accelerate the skin regeneration.					
32363671	6	64	theme	composite	834:842	arg1	film					844:847	G-CS composite film	829:847	G-CS composite film	829:847	It was found that G-HA composite film had better short-term and long-term stability compared with G-CS composite film.					
32363671	9	65	dep	in	1311:1312	arg1	vivo					1314:1317	vivo	1314:1317	vivo	1314:1317	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	7	66	contain	had	879:881	arg2	safety					901:906	better biological safety	883:906	better biological safety	883:906	And G-HA composite film also had better biological safety than G-CS film.					
32363671	7	66	contain	had	879:881	arg1	film					869:872	G-HA composite film	854:872	G-HA composite film	854:872	And G-HA composite film also had better biological safety than G-CS film.					
32363671	9	67	theme	promising	1259:1267	arg1	system					1278:1283	a promising delivery system	1257:1283	a promising delivery system of stem cells for further in vivo studies	1257:1325	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	3	68	theme	polysaccharide	287:300	arg1	modification					302:313	polysaccharide modification	287:313	polysaccharide modification	287:313	Although polysaccharide modification can improve the mechanical property and biological activity of gelatin films, information about the stability and cellular affinity is still limited.					
32363671	1	69	theme	biomedical	128:137	arg1	applications					139:150	biomedical applications	128:150	biomedical applications	128:150	The gelatin film has great potential in biomedical applications, especially in wound healing.					
32363671	8	70	link	jelly-derived	1037:1049	arg1	cells					1068:1072	jelly-derived mesenchymal stem cells	1037:1072	human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface	1006:1112	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	8	70	link	jelly-derived	1037:1049	arg1	MSCs					1078:1081	WJ MSCs	1075:1081	WJ MSCs	1075:1081	Moreover, the surface of G-HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ MSCs) better than G-CS film surface.					
32363671	0	71	theme	composite	71:79	arg1	films					81:85	gelatin-polysaccharide composite films	48:85	gelatin-polysaccharide composite films	48:85	Study on the stability and cellular affinity of gelatin-polysaccharide composite films.					
32363671	9	72	theme	delivery	1269:1276	arg1	system					1278:1283	a promising delivery system	1257:1283	a promising delivery system of stem cells for further in vivo studies	1257:1325	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32363671	6	73	theme	G-HA	749:752	arg1	film					764:767	G-HA composite film	749:767	G-HA composite film	749:767	It was found that G-HA composite film had better short-term and long-term stability compared with G-CS composite film.					
32363671	3	74	theme	biological	355:364	arg1	activity					366:373	biological activity	355:373	biological activity	355:373	Although polysaccharide modification can improve the mechanical property and biological activity of gelatin films, information about the stability and cellular affinity is still limited.					
32363671	9	75	theme	stem	1288:1291	arg1	cells					1293:1297	stem cells	1288:1297	stem cells	1288:1297	These data illustrated that G-HA composite film has better stability and cellular affinity compared with G-CS film, which could be considered a promising delivery system of stem cells for further in vivo studies.					
32577644	0	0	from	Roles	22:26	arg1	Protein					59:65	SARS-CoV-2 Spike Protein	42:65	SARS-CoV-2 Spike Protein	42:65	Beyond Shielding: The Roles of Glycans in SARS-CoV-2 Spike Protein.					
32577644	7	1	theme	interferometry	1162:1175	arg1	experiments					1177:1187	biolayer interferometry experiments	1153:1187	biolayer interferometry experiments	1153:1187	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift towards the "down" state.					
32577644	8	2	theme	therapeutic	1545:1555	arg1	efforts					1557:1563	therapeutic efforts	1545:1563	therapeutic efforts targeting this molecular machine	1545:1596	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	3	3	theme	other	474:478	arg1	viruses					480:486	many other viruses	469:486	many other viruses	469:486	Similar to many other viruses, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
32577644	1	4	theme	worldwide	236:244	arg1	infections					206:215	more than 15,000,000 infections	185:215	more than 15,000,000 infections	185:215	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	6	5	theme	ACE2	1103:1106	arg1	recognition					1108:1118	ACE2 recognition	1103:1118	ACE2 recognition	1103:1118	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	9	6	theme	vaccine	1830:1836	arg1	development					1838:1848	vaccine development	1830:1848	vaccine development	1830:1848	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
32577644	3	7	theme	SARS-CoV-2	493:502	arg1	spike					504:508	the SARS-CoV-2 spike	489:508	the SARS-CoV-2 spike	489:508	Similar to many other viruses, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
32577644	7	8	theme	conformational	1327:1340	arg1	shift					1342:1346	the RBD conformational shift	1319:1346	the RBD conformational shift towards the "down" state	1319:1371	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift towards the "down" state.					
32577644	4	9	theme	SARS-CoV-2	622:631	arg1	protein					635:641	glycosylated SARS-CoV-2 S protein	609:641	glycosylated SARS-CoV-2 S protein	609:641	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	5	10	theme	atomistic	828:836	arg1	perspective					838:848	an atomistic perspective	825:848	an atomistic perspective on the roles of glycans, and the protein structure and dynamics	825:912	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	5	11	theme	protein	883:889	arg1	structure					891:899	the protein structure	879:899	the protein structure	879:899	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	5	11	theme	protein	883:889	arg1	roles					857:861	the roles	853:861	the roles of glycans	853:872	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	9	12	theme	unseen	1636:1641	arg1	insights					1669:1676	hitherto unseen functional and structural insights	1627:1676	hitherto unseen functional and structural insights into the SARS-CoV-2 S protein	1627:1706	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
32577644	4	13	theme	full-length	588:598	arg1	model					600:604	a full-length model	586:604	a full-length model of glycosylated SARS-CoV-2 S protein	586:641	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	3	14	theme	immune	554:559	arg1	response					561:568	the host immune response	545:568	the host immune response	545:568	Similar to many other viruses, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
32577644	5	15	theme	glycans	866:872	arg1	structure					891:899	the protein structure	879:899	the protein structure	879:899	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	5	15	theme	glycans	866:872	arg1	roles					857:861	the roles	853:861	the roles of glycans	853:872	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	5	15	theme	glycans	866:872	arg1	dynamics					905:912	dynamics	905:912	dynamics	905:912	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	8	16	dep	glycan	1480:1485	arg1	shield					1487:1492	shield	1487:1492	shield	1487:1492	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	1	17	theme	acute	115:119	arg1	coronavirus					142:152	severe acute respiratory syndrome coronavirus 2	108:154	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	108:167	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	1	17	theme	acute	115:119	arg1	SARS-CoV-2					157:166	SARS-CoV-2	157:166	SARS-CoV-2	157:166	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	8	18	theme	accessibility	1399:1411	arg1	analyses					1413:1420	end-to-end accessibility analyses	1388:1420	end-to-end accessibility analyses	1388:1420	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	4	19	theme	protein	635:641	arg1	model					600:604	a full-length model	586:604	a full-length model of glycosylated SARS-CoV-2 S protein	586:641	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	7	20	theme	biolayer	1153:1160	arg1	experiments					1177:1187	biolayer interferometry experiments	1153:1187	biolayer interferometry experiments	1153:1187	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift towards the "down" state.					
32577644	3	21	dep	glycan	521:526	arg1	shield					528:533	shield	528:533	shield	528:533	Similar to many other viruses, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
32577644	9	22	theme	S	1698:1698	arg1	protein					1700:1706	the SARS-CoV-2 S protein	1683:1706	the SARS-CoV-2 S protein	1683:1706	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
32577644	6	23	theme	structural	938:947	arg1	role					949:952	an essential structural role	925:952	an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	925:1118	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	8	24	theme	complete	1432:1439	arg1	overview					1441:1448	a complete overview	1430:1448	a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine	1430:1596	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	6	25	from	sites	970:974	arg1	N-glycans					957:965	N-glycans	957:965	N-glycans at sites N165 and N234	957:988	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	6	25	from	sites	970:974	arg1	role					949:952	an essential structural role	925:952	an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	925:1118	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	4	26	theme	available	695:703	arg1	data					731:734	the available structural and biological data	691:734	the available structural and biological data	691:734	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	5	27	from	perspective	838:848	arg1	structure					891:899	the protein structure	879:899	the protein structure	879:899	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	5	27	from	perspective	838:848	arg1	roles					857:861	the roles	853:861	the roles of glycans	853:872	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	5	27	from	perspective	838:848	arg1	dynamics					905:912	dynamics	905:912	dynamics	905:912	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	9	28	theme	glycan	1716:1721	arg1	coat					1723:1726	its glycan coat	1712:1726	its glycan coat	1712:1726	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
32577644	6	29	theme	conformational	1008:1021	arg1	dynamics					1023:1030	the conformational dynamics	1004:1030	the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	1004:1118	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	2	30	theme	angiotensin-converting	418:439	arg1	enzyme					441:446	angiotensin-converting enzyme 2	418:448	the angiotensin-converting enzyme 2 (ACE2)	414:455	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates the host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
32577644	2	30	theme	angiotensin-converting	418:439	arg1	ACE2					451:454	ACE2	451:454	ACE2	451:454	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates the host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
32577644	8	31	theme	vulnerabilities	1457:1471	arg1	overview					1441:1448	a complete overview	1430:1448	a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine	1430:1596	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	6	32	from	role	949:952	arg1	N234					985:988	N234	985:988	N234	985:988	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	6	32	from	role	949:952	arg1	sites					970:974	sites N165 and N234	970:988	sites	970:974	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	1	33	theme	ongoing	72:78	arg1	pandemic					89:96	The ongoing COVID-19 pandemic	68:96	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	68:167	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	2	34	dep	spike	346:350	arg1	S					353:353	S	353:353	S	353:353	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates the host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
32577644	5	35	theme	molecular	773:781	arg1	dynamics					783:790	all-atom molecular dynamics	764:790	all-atom molecular dynamics	764:790	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	2	36	theme	cell	389:392	arg1	entry					394:398	the host cell entry	380:398	the host cell entry	380:398	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates the host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
32577644	7	37	theme	glycans	1224:1230	arg1	deletion					1206:1213	deletion	1206:1213	deletion of these glycans through N165A and N234A mutations	1206:1264	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift towards the "down" state.					
32577644	8	38	theme	SARS-CoV-2	1497:1506	arg1	protein					1510:1516	SARS-CoV-2 S protein	1497:1516	SARS-CoV-2 S protein	1497:1516	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	0	39	theme	SARS-CoV-2	42:51	arg1	Protein					59:65	SARS-CoV-2 Spike Protein	42:65	SARS-CoV-2 Spike Protein	42:65	Beyond Shielding: The Roles of Glycans in SARS-CoV-2 Spike Protein.					
32577644	2	40	theme	spike	346:350	arg1	protein					356:362	the extensively glycosylated SARS-CoV-2 spike (S) protein	306:362	the extensively glycosylated SARS-CoV-2 spike (S) protein	306:362	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates the host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
32577644	5	41	theme	Multiple	737:744	arg1	simulations					792:802	Multiple microsecond-long, all-atom molecular dynamics simulations	737:802	simulations	792:802	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	0	42	dep	Roles	22:26	arg1	Shielding					7:15	Shielding	7:15	Shielding	7:15	Beyond Shielding: The Roles of Glycans in SARS-CoV-2 Spike Protein.					
32577644	6	43	theme	domain	1064:1069	arg1	dynamics					1023:1030	the conformational dynamics	1004:1030	the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	1004:1118	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	8	44	theme	protein	1510:1516	arg1	glycan					1480:1485	the glycan shield	1476:1492	the glycan shield	1476:1492	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	2	45	theme	glycosylated	322:333	arg1	protein					356:362	the extensively glycosylated SARS-CoV-2 spike (S) protein	306:362	the extensively glycosylated SARS-CoV-2 spike (S) protein	306:362	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates the host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
32577644	6	46	theme	receptor	1047:1054	arg1	RBD					1072:1074	RBD	1072:1074	RBD	1072:1074	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	6	46	theme	receptor	1047:1054	arg1	domain					1064:1069	the spike's receptor binding domain	1035:1069	the spike's receptor binding domain (RBD)	1035:1075	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	7	47	dep	N165A	1240:1244	arg1	mutations					1256:1264	mutations	1256:1264	mutations	1256:1264	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift towards the "down" state.					
32577644	3	48	theme	many	469:472	arg1	viruses					480:486	many other viruses	469:486	many other viruses	469:486	Similar to many other viruses, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
32577644	4	49	gly	glycosylated	609:620	arg1	protein					635:641	glycosylated SARS-CoV-2 S protein	609:641	glycosylated SARS-CoV-2 S protein	609:641	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	5	50	used	used	809:812	arg2	simulations					792:802	Multiple microsecond-long, all-atom molecular dynamics simulations	737:802	simulations	792:802	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	2	51	theme	Antibody	255:262	arg1	development					264:274	Antibody development	255:274	Antibody development	255:274	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates the host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
32577644	0	52	theme	Glycans	31:37	arg1	Roles					22:26	The Roles	18:26	The Roles of Glycans in SARS-CoV-2 Spike Protein	18:65	Beyond Shielding: The Roles of Glycans in SARS-CoV-2 Spike Protein.					
32577644	7	53	theme	"	1365:1365	arg1	state					1367:1371	the "down" state	1356:1371	the "down" state	1356:1371	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift towards the "down" state.					
32577644	7	54	theme	shift	1342:1346	arg1	result					1309:1314	a result	1307:1314	a result of the RBD conformational shift towards the "down" state	1307:1371	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift towards the "down" state.					
32577644	7	54	theme	shift	1342:1346	arg1	binding					1288:1294	binding	1288:1294	binding to ACE2	1288:1302	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift towards the "down" state.					
32577644	4	55	theme	glycosylated	609:620	arg1	protein					635:641	glycosylated SARS-CoV-2 S protein	609:641	glycosylated SARS-CoV-2 S protein	609:641	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	6	56	from	N234	985:988	arg1	N-glycans					957:965	N-glycans	957:965	N-glycans at sites N165 and N234	957:988	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	6	56	from	N234	985:988	arg1	role					949:952	an essential structural role	925:952	an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	925:1118	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	7	57	theme	RBD	1323:1325	arg1	shift					1342:1346	the RBD conformational shift	1319:1346	the RBD conformational shift towards the "down" state	1319:1371	This finding is corroborated by biolayer interferometry experiments, which show that deletion of these glycans through N165A and N234A mutations significantly reduces binding to ACE2 as a result of the RBD conformational shift towards the "down" state.					
32577644	3	58	theme	host	549:552	arg1	response					561:568	the host immune response	545:568	the host immune response	545:568	Similar to many other viruses, the SARS-CoV-2 spike utilizes a glycan shield to thwart the host immune response.					
32577644	9	59	theme	functional	1643:1652	arg1	insights					1669:1676	hitherto unseen functional and structural insights	1627:1676	hitherto unseen functional and structural insights into the SARS-CoV-2 S protein	1627:1706	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
32577644	9	60	theme	structural	1658:1667	arg1	insights					1669:1676	hitherto unseen functional and structural insights	1627:1676	hitherto unseen functional and structural insights into the SARS-CoV-2 S protein	1627:1706	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
32577644	8	61	theme	end-to-end	1388:1397	arg1	analyses					1413:1420	end-to-end accessibility analyses	1388:1420	end-to-end accessibility analyses	1388:1420	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	1	62	theme	severe	108:113	arg1	coronavirus					142:152	severe acute respiratory syndrome coronavirus 2	108:154	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	108:167	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	1	62	theme	severe	108:113	arg1	SARS-CoV-2					157:166	SARS-CoV-2	157:166	SARS-CoV-2	157:166	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	4	63	theme	open	656:659	arg1	states					672:677	the open and closed states	652:677	the open and closed states	652:677	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	0	64	theme	Spike	53:57	arg1	Protein					59:65	SARS-CoV-2 Spike Protein	42:65	SARS-CoV-2 Spike Protein	42:65	Beyond Shielding: The Roles of Glycans in SARS-CoV-2 Spike Protein.					
32577644	1	65	theme	respiratory	121:131	arg1	coronavirus					142:152	severe acute respiratory syndrome coronavirus 2	108:154	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	108:167	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	1	65	theme	respiratory	121:131	arg1	SARS-CoV-2					157:166	SARS-CoV-2	157:166	SARS-CoV-2	157:166	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	6	66	theme	N-glycans	957:965	arg1	role					949:952	an essential structural role	925:952	an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	925:1118	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	9	67	theme	SARS-CoV-2	1687:1696	arg1	protein					1700:1706	the SARS-CoV-2 S protein	1683:1706	the SARS-CoV-2 S protein	1683:1706	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
32577644	4	68	theme	S	633:633	arg1	protein					635:641	glycosylated SARS-CoV-2 S protein	609:641	glycosylated SARS-CoV-2 S protein	609:641	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	6	69	theme	essential	928:936	arg1	role					949:952	an essential structural role	925:952	an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition	925:1118	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	4	70	theme	structural	705:714	arg1	data					731:734	the available structural and biological data	691:734	the available structural and biological data	691:734	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	5	71	theme	microsecond-long	746:761	arg1	simulations					792:802	Multiple microsecond-long, all-atom molecular dynamics simulations	737:802	simulations	792:802	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	8	72	theme	glycan	1480:1485	arg1	vulnerabilities					1457:1471	the vulnerabilities	1453:1471	the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine	1453:1596	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	4	73	theme	closed	665:670	arg1	states					672:677	the open and closed states	652:677	the open and closed states	652:677	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	5	74	theme	all-atom	764:771	arg1	dynamics					783:790	all-atom molecular dynamics	764:790	all-atom molecular dynamics	764:790	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32577644	1	75	theme	COVID-19	80:87	arg1	pandemic					89:96	The ongoing COVID-19 pandemic	68:96	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	68:167	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	8	76	theme	S	1508:1508	arg1	protein					1510:1516	SARS-CoV-2 S protein	1497:1516	SARS-CoV-2 S protein	1497:1516	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	2	77	theme	host	384:387	arg1	entry					394:398	the host cell entry	380:398	the host cell entry	380:398	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates the host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
32577644	4	78	dep	built	580:584	arg1	both					644:647	both	644:647	both	644:647	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	9	79	theme	conformational	1765:1778	arg1	plasticity					1780:1789	the conformational plasticity	1761:1789	the conformational plasticity of the RBD that could be harnessed for vaccine development	1761:1848	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
32577644	1	80	theme	syndrome	133:140	arg1	coronavirus					142:152	severe acute respiratory syndrome coronavirus 2	108:154	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	108:167	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	1	80	theme	syndrome	133:140	arg1	SARS-CoV-2					157:166	SARS-CoV-2	157:166	SARS-CoV-2	157:166	The ongoing COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in more than 15,000,000 infections and 600,000 deaths worldwide to date.					
32577644	8	81	theme	molecular	1580:1588	arg1	machine					1590:1596	this molecular machine	1575:1596	this molecular machine	1575:1596	Additionally, end-to-end accessibility analyses outline a complete overview of the vulnerabilities of the glycan shield of SARS-CoV-2 S protein, which may be exploited by therapeutic efforts targeting this molecular machine.					
32577644	6	82	theme	binding	1056:1062	arg1	RBD					1072:1074	RBD	1072:1074	RBD	1072:1074	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	6	82	theme	binding	1056:1062	arg1	domain					1064:1069	the spike's receptor binding domain	1035:1069	the spike's receptor binding domain (RBD)	1035:1075	We reveal an essential structural role of N-glycans at sites N165 and N234 in modulating the conformational dynamics of the spike's receptor binding domain (RBD), which is responsible for ACE2 recognition.					
32577644	2	83	gly	glycosylated	322:333	arg1	protein					356:362	the extensively glycosylated SARS-CoV-2 spike (S) protein	306:362	the extensively glycosylated SARS-CoV-2 spike (S) protein	306:362	Antibody development efforts mainly revolve around the extensively glycosylated SARS-CoV-2 spike (S) protein, which mediates the host cell entry by binding to the angiotensin-converting enzyme 2 (ACE2).					
32577644	4	84	theme	biological	720:729	arg1	data					731:734	the available structural and biological data	691:734	the available structural and biological data	691:734	Here, we built a full-length model of glycosylated SARS-CoV-2 S protein, both in the open and closed states, augmenting the available structural and biological data.					
32577644	9	85	theme	RBD	1798:1800	arg1	plasticity					1780:1789	the conformational plasticity	1761:1789	the conformational plasticity of the RBD that could be harnessed for vaccine development	1761:1848	Overall, this work presents hitherto unseen functional and structural insights into the SARS-CoV-2 S protein and its glycan coat, providing a strategy to control the conformational plasticity of the RBD that could be harnessed for vaccine development.					
32577644	5	86	dep	simulations	792:802	arg1	dynamics					783:790	all-atom molecular dynamics	764:790	all-atom molecular dynamics	764:790	Multiple microsecond-long, all-atom molecular dynamics simulations were used to provide an atomistic perspective on the roles of glycans, and the protein structure and dynamics.					
32615219	7	0	theme	relative	1467:1474	arg1	abundance					1476:1484	the relative abundance	1463:1484	the relative abundance of Proteobacteria	1463:1502	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	6	1	theme	long	1248:1251	arg1	term					1253:1256	the long term	1244:1256	the long term of HFD	1244:1263	Meanwhile, the relative abundance of gut microbiota could be disordered by the long term of HFD.					
32615219	8	2	theme	serum	1696:1700	arg1	profiles					1708:1715	serum lipid profiles	1696:1715	serum lipid profiles	1696:1715	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
32615219	9	3	theme	gut	1939:1941	arg1	microbiota					1943:1952	gut microbiota	1939:1952	gut microbiota	1939:1952	Taken together, the present study demonstrated that quinoa polysaccharide supplementation could ameliorate the hyperlipidemia induced by HFD in association with modulating gut microbiota in a positive way.					
32615219	2	4	theme	functional	164:173	arg1	values					175:180	the high nutritional and functional values	139:180	the high nutritional and functional values of quinoa	139:190	As the high nutritional and functional values of quinoa acknowledged, the increasing researches focus on the bioactivities and related mechanisms of its abundant carbohydrates.					
32615219	2	5	theme	carbohydrates	298:310	arg1	bioactivities					245:257	bioactivities	245:257	bioactivities	245:257	As the high nutritional and functional values of quinoa acknowledged, the increasing researches focus on the bioactivities and related mechanisms of its abundant carbohydrates.					
32615219	2	5	theme	carbohydrates	298:310	arg1	mechanisms					271:280	related mechanisms	263:280	related mechanisms	263:280	As the high nutritional and functional values of quinoa acknowledged, the increasing researches focus on the bioactivities and related mechanisms of its abundant carbohydrates.					
32615219	2	6	theme	nutritional	148:158	arg1	values					175:180	the high nutritional and functional values	139:180	the high nutritional and functional values of quinoa	139:190	As the high nutritional and functional values of quinoa acknowledged, the increasing researches focus on the bioactivities and related mechanisms of its abundant carbohydrates.					
32615219	5	7	from	accumulation	1155:1166	arg1	rats					1099:1102	rats	1099:1102	rats fed with HFD	1099:1115	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	8	theme	600 mg·kg-1·day-1	818:834	arg1	polysaccharide					778:791	quinoa polysaccharide	771:791	quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1	771:834	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	7	9	theme	microbiota	1381:1390	arg1	structure					1402:1410	the gut microbiota community structure	1373:1410	the gut microbiota community structure	1373:1410	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	9	10	theme	positive	1959:1966	arg1	way					1968:1970	a positive way	1957:1970	a positive way	1957:1970	Taken together, the present study demonstrated that quinoa polysaccharide supplementation could ameliorate the hyperlipidemia induced by HFD in association with modulating gut microbiota in a positive way.					
32615219	6	11	theme	microbiota	1210:1219	arg1	abundance					1193:1201	the relative abundance	1180:1201	the relative abundance of gut microbiota	1180:1219	Meanwhile, the relative abundance of gut microbiota could be disordered by the long term of HFD.					
32615219	6	11	theme	microbiota	1210:1219	arg1	Meanwhile					1169:1177	Meanwhile	1169:1177	Meanwhile	1169:1177	Meanwhile, the relative abundance of gut microbiota could be disordered by the long term of HFD.					
32615219	5	12	theme	glutamic	1056:1063	arg1	triglyceride					923:934	serum total triglyceride	911:934	serum total triglyceride (TG)	911:939	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	12	theme	glutamic	1056:1063	arg1	transaminase					1077:1088	glutamic oxaloacetic transaminase	1056:1088	glutamic oxaloacetic transaminase	1056:1088	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	6	13	theme	gut	1206:1208	arg1	microbiota					1210:1219	gut microbiota	1206:1219	gut microbiota	1206:1219	Meanwhile, the relative abundance of gut microbiota could be disordered by the long term of HFD.					
32615219	2	14	dep	bioactivities	245:257	arg1	the					241:243	the	241:243	the	241:243	As the high nutritional and functional values of quinoa acknowledged, the increasing researches focus on the bioactivities and related mechanisms of its abundant carbohydrates.					
32615219	4	15	dep	technology	604:613	arg1	yield					616:620	yield	616:620	yield	616:620	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	7	16	theme	species	1343:1349	arg1	richness					1351:1358	species richness	1343:1358	species richness	1343:1358	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	4	17	theme	molar	720:724	arg1	1.17:1					712:717	1.17:1	712:717	1.17:1	712:717	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	4	17	theme	molar	720:724	arg1	ratio					726:730	molar ratio	720:730	molar ratio	720:730	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	3	18	from	effects	336:342	arg1	quinoa					388:393	quinoa	388:393	quinoa	388:393	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	5	19	from	levels	901:906	arg1	rats					1099:1102	rats	1099:1102	rats fed with HFD	1099:1115	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	20	theme	low	942:944	arg1	cholesterol					966:976	low density lipoprotein cholesterol	942:976	low density lipoprotein cholesterol (LDL-C)	942:984	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	20	theme	low	942:944	arg1	triglyceride					923:934	serum total triglyceride	911:934	serum total triglyceride (TG)	911:939	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	20	theme	low	942:944	arg1	LDL-C					979:983	LDL-C	979:983	LDL-C	979:983	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	7	21	theme	quinoa	1307:1312	arg1	polysaccharide					1314:1327	quinoa polysaccharide	1307:1327	quinoa polysaccharide	1307:1327	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	5	22	theme	oral	748:751	arg1	administration					753:766	the oral administration	744:766	the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks	744:846	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	23	theme	total	917:921	arg1	malondialdehyde					987:1001	malondialdehyde	987:1001	malondialdehyde (MDA)	987:1007	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	23	theme	total	917:921	arg1	triglyceride					923:934	serum total triglyceride	911:934	serum total triglyceride (TG)	911:939	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	23	theme	total	917:921	arg1	transaminase					1033:1044	total glutamic pyruvic transaminase	1010:1044	total glutamic pyruvic transaminase (ALT)	1010:1050	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	23	theme	total	917:921	arg1	cholesterol					966:976	low density lipoprotein cholesterol	942:976	low density lipoprotein cholesterol (LDL-C)	942:984	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	23	theme	total	917:921	arg1	transaminase					1077:1088	glutamic oxaloacetic transaminase	1056:1088	glutamic oxaloacetic transaminase	1056:1088	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	23	theme	total	917:921	arg1	TG					937:938	TG	937:938	TG	937:938	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	3	24	theme	polysaccharide	359:372	arg1	fraction					374:381	the soluble polysaccharide fraction	347:381	the soluble polysaccharide fraction from quinoa	347:393	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	1	25	theme	diet-induced	79:90	arg1	hyperlipidemia					92:105	high-fat diet-induced hyperlipidemia	70:105	high-fat diet-induced hyperlipidemia	70:105	polysaccharides ameliorate high-fat diet-induced hyperlipidemia and modulate gut microbiota.					
32615219	3	26	theme	rats	440:443	arg1	lipid					431:435	the serum lipid	421:435	the serum lipid of rats treated by high-fat diet (HFD)	421:474	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	0	27	theme	Dietary	0:6	arg1	Willd					35:39	Willd	35:39	Willd	35:39	Dietary quinoa (Chenopodium quinoa Willd.)					
32615219	0	27	theme	Dietary	0:6	arg1	quinoa					8:13	Dietary quinoa	0:13	Dietary quinoa (Chenopodium quinoa Willd.)	0:41	Dietary quinoa (Chenopodium quinoa Willd.)					
32615219	5	28	theme	reduced	1133:1139	arg1	accumulation					1155:1166	the reduced hepatic lipid accumulation	1129:1166	the reduced hepatic lipid accumulation	1129:1166	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	29	theme	lipoprotein	954:964	arg1	cholesterol					966:976	low density lipoprotein cholesterol	942:976	low density lipoprotein cholesterol (LDL-C)	942:984	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	29	theme	lipoprotein	954:964	arg1	triglyceride					923:934	serum total triglyceride	911:934	serum total triglyceride (TG)	911:939	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	29	theme	lipoprotein	954:964	arg1	LDL-C					979:983	LDL-C	979:983	LDL-C	979:983	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	7	30	theme	dietary	1280:1286	arg1	supplementation					1288:1302	dietary supplementation	1280:1302	dietary supplementation of quinoa polysaccharide	1280:1327	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	9	31	theme	polysaccharide	1826:1839	arg1	supplementation					1841:1855	quinoa polysaccharide supplementation	1819:1855	quinoa polysaccharide supplementation	1819:1855	Taken together, the present study demonstrated that quinoa polysaccharide supplementation could ameliorate the hyperlipidemia induced by HFD in association with modulating gut microbiota in a positive way.					
32615219	8	32	theme	16S	1530:1532	arg1	gene					1539:1542	16S rRNA gene	1530:1542	16S rRNA gene	1530:1542	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
32615219	5	33	theme	lipid	1149:1153	arg1	accumulation					1155:1166	the reduced hepatic lipid accumulation	1129:1166	the reduced hepatic lipid accumulation	1129:1166	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	1	34	theme	gut	120:122	arg1	microbiota					124:133	gut microbiota	120:133	gut microbiota	120:133	polysaccharides ameliorate high-fat diet-induced hyperlipidemia and modulate gut microbiota.					
32615219	8	35	theme	gene	1539:1542	arg1	Sequencing					1516:1525	Sequencing	1516:1525	Sequencing of 16S rRNA gene	1516:1542	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
32615219	2	36	theme	quinoa	185:190	arg1	values					175:180	the high nutritional and functional values	139:180	the high nutritional and functional values of quinoa	139:190	As the high nutritional and functional values of quinoa acknowledged, the increasing researches focus on the bioactivities and related mechanisms of its abundant carbohydrates.					
32615219	5	37	theme	polysaccharide	778:791	arg1	administration					753:766	the oral administration	744:766	the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks	744:846	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	38	theme	glutamic	1016:1023	arg1	triglyceride					923:934	serum total triglyceride	911:934	serum total triglyceride (TG)	911:939	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	38	theme	glutamic	1016:1023	arg1	AST					1091:1093	AST	1091:1093	AST	1091:1093	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	38	theme	glutamic	1016:1023	arg1	ALT					1047:1049	ALT	1047:1049	ALT	1047:1049	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	38	theme	glutamic	1016:1023	arg1	transaminase					1033:1044	total glutamic pyruvic transaminase	1010:1044	total glutamic pyruvic transaminase (ALT)	1010:1050	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	39	theme	300 mg·kg-1·day-1	796:812	arg1	polysaccharide					778:791	quinoa polysaccharide	771:791	quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1	771:834	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	7	40	theme	Proteobacteria	1489:1502	arg1	abundance					1476:1484	the relative abundance	1463:1484	the relative abundance of Proteobacteria	1463:1502	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	2	41	theme	increasing	210:219	arg1	researches					221:230	the increasing researches	206:230	the increasing researches	206:230	As the high nutritional and functional values of quinoa acknowledged, the increasing researches focus on the bioactivities and related mechanisms of its abundant carbohydrates.					
32615219	8	42	theme	Allobaculum	1645:1655	arg1	abundances					1613:1622	the relative abundances	1600:1622	the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation	1600:1764	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
32615219	8	43	theme	polysaccharide	1575:1588	arg1	intake					1558:1563	intake	1558:1563	intake of quinoa polysaccharide	1558:1588	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
32615219	4	44	theme	%	629:629	arg1	yield					616:620	yield	616:620	yield	616:620	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	3	45	theme	gut	500:502	arg1	microbiota					504:513	the disordered gut microbiota	485:513	the disordered gut microbiota	485:513	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	7	46	theme	Bacteroides	1450:1460	arg1	ratio					1426:1430	the ratio	1422:1430	the ratio of Firmicutes and Bacteroides	1422:1460	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	4	47	theme	extraction	593:602	arg1	technology					604:613	ultrasonic-assisted extraction technology	573:613	ultrasonic-assisted extraction technology (yield of 9.65%)	573:630	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	7	48	theme	Firmicutes	1435:1444	arg1	ratio					1426:1430	the ratio	1422:1430	the ratio of Firmicutes and Bacteroides	1422:1460	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	8	49	theme	Desulfovibrio	1627:1639	arg1	abundances					1613:1622	the relative abundances	1600:1622	the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation	1600:1764	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
32615219	2	50	theme	abundant	289:296	arg1	carbohydrates					298:310	its abundant carbohydrates	285:310	its abundant carbohydrates	285:310	As the high nutritional and functional values of quinoa acknowledged, the increasing researches focus on the bioactivities and related mechanisms of its abundant carbohydrates.					
32615219	8	51	theme	lipid	1702:1706	arg1	profiles					1708:1715	serum lipid profiles	1696:1715	serum lipid profiles	1696:1715	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
32615219	4	52	theme	monosaccharide	657:670	arg1	composition					672:682	the monosaccharide composition	653:682	the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio)	653:731	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	2	53	theme	high	143:146	arg1	values					175:180	the high nutritional and functional values	139:180	the high nutritional and functional values of quinoa	139:190	As the high nutritional and functional values of quinoa acknowledged, the increasing researches focus on the bioactivities and related mechanisms of its abundant carbohydrates.					
32615219	7	54	theme	community	1392:1400	arg1	structure					1402:1410	the gut microbiota community structure	1373:1410	the gut microbiota community structure	1373:1410	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	7	55	theme	gut	1377:1379	arg1	structure					1402:1410	the gut microbiota community structure	1373:1410	the gut microbiota community structure	1373:1410	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	5	56	theme	oxaloacetic	1065:1075	arg1	triglyceride					923:934	serum total triglyceride	911:934	serum total triglyceride (TG)	911:939	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	56	theme	oxaloacetic	1065:1075	arg1	transaminase					1077:1088	glutamic oxaloacetic transaminase	1056:1088	glutamic oxaloacetic transaminase	1056:1088	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	8	57	theme	intestinal	1742:1751	arg1	inflammation					1753:1764	intestinal inflammation	1742:1764	intestinal inflammation	1742:1764	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
32615219	3	58	from	quinoa	388:393	arg1	fraction					374:381	the soluble polysaccharide fraction	347:381	the soluble polysaccharide fraction from quinoa	347:393	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	3	58	from	quinoa	388:393	arg1	effects					336:342	the beneficial effects	321:342	the beneficial effects of the soluble polysaccharide fraction from quinoa	321:393	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	3	59	theme	beneficial	325:334	arg1	effects					336:342	the beneficial effects	321:342	the beneficial effects of the soluble polysaccharide fraction from quinoa	321:393	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	6	60	theme	relative	1184:1191	arg1	abundance					1193:1201	the relative abundance	1180:1201	the relative abundance of gut microbiota	1180:1219	Meanwhile, the relative abundance of gut microbiota could be disordered by the long term of HFD.					
32615219	6	60	theme	relative	1184:1191	arg1	Meanwhile					1169:1177	Meanwhile	1169:1177	Meanwhile	1169:1177	Meanwhile, the relative abundance of gut microbiota could be disordered by the long term of HFD.					
32615219	6	61	theme	HFD	1261:1263	arg1	term					1253:1256	the long term	1244:1256	the long term of HFD	1244:1263	Meanwhile, the relative abundance of gut microbiota could be disordered by the long term of HFD.					
32615219	2	62	theme	related	263:269	arg1	mechanisms					271:280	related mechanisms	263:280	related mechanisms	263:280	As the high nutritional and functional values of quinoa acknowledged, the increasing researches focus on the bioactivities and related mechanisms of its abundant carbohydrates.					
32615219	5	63	theme	serum	911:915	arg1	malondialdehyde					987:1001	malondialdehyde	987:1001	malondialdehyde (MDA)	987:1007	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	63	theme	serum	911:915	arg1	triglyceride					923:934	serum total triglyceride	911:934	serum total triglyceride (TG)	911:939	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	63	theme	serum	911:915	arg1	transaminase					1033:1044	total glutamic pyruvic transaminase	1010:1044	total glutamic pyruvic transaminase (ALT)	1010:1050	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	63	theme	serum	911:915	arg1	cholesterol					966:976	low density lipoprotein cholesterol	942:976	low density lipoprotein cholesterol (LDL-C)	942:984	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	63	theme	serum	911:915	arg1	transaminase					1077:1088	glutamic oxaloacetic transaminase	1056:1088	glutamic oxaloacetic transaminase	1056:1088	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	63	theme	serum	911:915	arg1	TG					937:938	TG	937:938	TG	937:938	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	3	64	theme	soluble	351:357	arg1	fraction					374:381	the soluble polysaccharide fraction	347:381	the soluble polysaccharide fraction from quinoa	347:393	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	7	65	theme	polysaccharide	1314:1327	arg1	supplementation					1288:1302	dietary supplementation	1280:1302	dietary supplementation of quinoa polysaccharide	1280:1327	Nevertheless, dietary supplementation of quinoa polysaccharide could enhance species richness and regulate the gut microbiota community structure, reducing the ratio of Firmicutes and Bacteroides, the relative abundance of Proteobacteria.					
32615219	5	66	theme	triglyceride	923:934	arg1	levels					901:906	the levels	897:906	the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD	897:1115	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	66	theme	triglyceride	923:934	arg1	accumulation					1155:1166	the reduced hepatic lipid accumulation	1129:1166	the reduced hepatic lipid accumulation	1129:1166	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	9	67	theme	present	1787:1793	arg1	study					1795:1799	the present study	1783:1799	the present study	1783:1799	Taken together, the present study demonstrated that quinoa polysaccharide supplementation could ameliorate the hyperlipidemia induced by HFD in association with modulating gut microbiota in a positive way.					
32615219	1	68	theme	high-fat	70:77	arg1	hyperlipidemia					92:105	high-fat diet-induced hyperlipidemia	70:105	high-fat diet-induced hyperlipidemia	70:105	polysaccharides ameliorate high-fat diet-induced hyperlipidemia and modulate gut microbiota.					
32615219	3	69	theme	fraction	374:381	arg1	effects					336:342	the beneficial effects	321:342	the beneficial effects of the soluble polysaccharide fraction from quinoa	321:393	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	3	70	theme	high-fat	456:463	arg1	HFD					471:473	HFD	471:473	HFD	471:473	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	3	70	theme	high-fat	456:463	arg1	diet					465:468	high-fat diet	456:468	high-fat diet (HFD)	456:474	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	5	71	theme	density	946:952	arg1	cholesterol					966:976	low density lipoprotein cholesterol	942:976	low density lipoprotein cholesterol (LDL-C)	942:984	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	71	theme	density	946:952	arg1	triglyceride					923:934	serum total triglyceride	911:934	serum total triglyceride (TG)	911:939	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	71	theme	density	946:952	arg1	LDL-C					979:983	LDL-C	979:983	LDL-C	979:983	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	9	72	theme	quinoa	1819:1824	arg1	supplementation					1841:1855	quinoa polysaccharide supplementation	1819:1855	quinoa polysaccharide supplementation	1819:1855	Taken together, the present study demonstrated that quinoa polysaccharide supplementation could ameliorate the hyperlipidemia induced by HFD in association with modulating gut microbiota in a positive way.					
32615219	8	73	theme	rRNA	1534:1537	arg1	gene					1539:1542	16S rRNA gene	1530:1542	16S rRNA gene	1530:1542	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
32615219	0	74	theme	Chenopodium	16:26	arg1	quinoa					28:33	Chenopodium quinoa	16:33	Chenopodium quinoa Willd.	16:40	Dietary quinoa (Chenopodium quinoa Willd.)					
32615219	5	75	theme	hepatic	1141:1147	arg1	accumulation					1155:1166	the reduced hepatic lipid accumulation	1129:1166	the reduced hepatic lipid accumulation	1129:1166	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	4	76	theme	ultrasonic-assisted	573:591	arg1	technology					604:613	ultrasonic-assisted extraction technology	573:613	ultrasonic-assisted extraction technology (yield of 9.65%)	573:630	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	5	77	theme	quinoa	771:776	arg1	polysaccharide					778:791	quinoa polysaccharide	771:791	quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1	771:834	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	78	theme	total	1010:1014	arg1	triglyceride					923:934	serum total triglyceride	911:934	serum total triglyceride (TG)	911:939	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	78	theme	total	1010:1014	arg1	AST					1091:1093	AST	1091:1093	AST	1091:1093	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	78	theme	total	1010:1014	arg1	ALT					1047:1049	ALT	1047:1049	ALT	1047:1049	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	78	theme	total	1010:1014	arg1	transaminase					1033:1044	total glutamic pyruvic transaminase	1010:1044	total glutamic pyruvic transaminase (ALT)	1010:1050	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	4	79	theme	polysaccharide	525:538	arg1	faction					540:546	The polysaccharide faction	521:546	The polysaccharide faction	521:546	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	4	80	with	composition	672:682	arg1	arabinose					701:709	arabinose	701:709	arabinose	701:709	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	4	80	with	composition	672:682	arg1	glucose					689:695	glucose	689:695	glucose	689:695	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	5	81	theme	pyruvic	1025:1031	arg1	triglyceride					923:934	serum total triglyceride	911:934	serum total triglyceride (TG)	911:939	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	81	theme	pyruvic	1025:1031	arg1	AST					1091:1093	AST	1091:1093	AST	1091:1093	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	81	theme	pyruvic	1025:1031	arg1	ALT					1047:1049	ALT	1047:1049	ALT	1047:1049	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	5	81	theme	pyruvic	1025:1031	arg1	transaminase					1033:1044	total glutamic pyruvic transaminase	1010:1044	total glutamic pyruvic transaminase (ALT)	1010:1050	And then, the oral administration of quinoa polysaccharide of 300 mg·kg-1·day-1 and 600 mg·kg-1·day-1 for 8 weeks remarkably alleviated dyslipidemia by decreasing the levels of serum total triglyceride (TG), low density lipoprotein cholesterol (LDL-C), malondialdehyde (MDA), total glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in rats fed with HFD, as well as the reduced hepatic lipid accumulation.					
32615219	3	82	theme	serum	425:429	arg1	lipid					431:435	the serum lipid	421:435	the serum lipid of rats treated by high-fat diet (HFD)	421:474	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	8	83	theme	quinoa	1568:1573	arg1	polysaccharide					1575:1588	quinoa polysaccharide	1568:1588	quinoa polysaccharide	1568:1588	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
32615219	3	84	theme	disordered	489:498	arg1	microbiota					504:513	the disordered gut microbiota	485:513	the disordered gut microbiota	485:513	Herein, the beneficial effects of the soluble polysaccharide fraction from quinoa was investigated to lower the serum lipid of rats treated by high-fat diet (HFD) and call the disordered gut microbiota back.					
32615219	4	85	dep	glucose	689:695	arg1	1.17:1					712:717	1.17:1	712:717	1.17:1	712:717	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	4	85	dep	glucose	689:695	arg1	ratio					726:730	molar ratio	720:730	molar ratio	720:730	The polysaccharide faction was firstly extracted by ultrasonic-assisted extraction technology (yield of 9.65%) and characterized of the monosaccharide composition with glucose and arabinose (1.17:1, molar ratio).					
32615219	8	86	theme	relative	1604:1611	arg1	abundances					1613:1622	the relative abundances	1600:1622	the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation	1600:1764	Meanwhile, Sequencing of 16S rRNA gene revealed that intake of quinoa polysaccharide decreased the relative abundances of Desulfovibrio and Allobaculum, which were positively correlated with serum lipid profiles and beneficial to lessen intestinal inflammation.					
34684694	3	0	theme	fish	503:506	arg1	oils					508:511	omega fish oils	497:511	omega fish oils	497:511	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	4	1	theme	smelt	596:600	arg1	oil					607:609	sand smelt fish oil	591:609	sand smelt fish oil	591:609	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	10	2	theme	encapsulating	1714:1726	arg1	agents					1728:1733	all the encapsulating agents	1706:1733	all the encapsulating agents	1706:1733	It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control).					
34684694	1	3	theme	omega-3	148:154	arg1	nutrients					189:197	important nutrients	179:197	important nutrients that assist in the prevention and treatment of heart disease	179:258	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	1	3	theme	omega-3	148:154	arg1	acids					162:166	long-chain omega-3 fatty acids	137:166	long-chain omega-3 fatty acids	137:166	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	9	4	theme	oil	1520:1522	arg1	values					1474:1479	peroxide values	1465:1479	peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils	1465:1565	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	9	4	theme	oil	1520:1522	arg1	PV					1482:1483	PV	1482:1483	PV	1482:1483	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	1	5	theme	acids	162:166	arg1	oil					108:110	Fish oil	103:110	Fish oil	103:110	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	1	5	theme	acids	162:166	arg1	source					127:132	the primary source	115:132	the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits	115:288	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	9	6	theme	sand	1528:1531	arg1	smelt					1533:1537	sand smelt	1528:1537	sand smelt	1528:1537	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	3	7	dep	efficiency	459:468	arg1	the					441:443	the	441:443	the	441:443	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	10	8	theme	protective	1746:1755	arg1	effect					1757:1762	a protective effect	1744:1762	a protective effect	1744:1762	It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control).					
34684694	1	9	contain	have	264:267	arg2	benefits					281:288	many health benefits	269:288	many health benefits	269:288	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	1	9	contain	have	264:267	arg1	nutrients					189:197	important nutrients	179:197	important nutrients that assist in the prevention and treatment of heart disease	179:258	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	1	9	contain	have	264:267	arg1	acids					162:166	long-chain omega-3 fatty acids	137:166	long-chain omega-3 fatty acids	137:166	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	8	10	theme	%	1343:1343	arg1	EE					1324:1325	EE	1324:1325	EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively	1324:1411	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	8	11	theme	whey	1188:1191	arg1	protein					1193:1199	whey protein	1188:1199	whey protein	1188:1199	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	1	12	theme	important	179:187	arg1	nutrients					189:197	important nutrients	179:197	important nutrients that assist in the prevention and treatment of heart disease	179:258	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	1	12	theme	important	179:187	arg1	acids					162:166	long-chain omega-3 fatty acids	137:166	long-chain omega-3 fatty acids	137:166	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	4	13	theme	wall	648:651	arg1	maltodextrin					699:710	maltodextrin	699:710	maltodextrin	699:710	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	13	theme	wall	648:651	arg1	materials					653:661	three different wall materials	632:661	three different wall materials (whey protein, gum Arabic (AG), and maltodextrin)	632:711	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	13	theme	wall	648:651	arg1	protein					669:675	whey protein	664:675	whey protein	664:675	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	13	theme	wall	648:651	arg1	Arabic					682:687	gum Arabic	678:687	gum Arabic (AG)	678:692	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	10	14	theme	encapsulated	1771:1782	arg1	oil					1789:1791	the encapsulated fish oil	1767:1791	the encapsulated fish oil	1767:1791	It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control).					
34684694	1	15	theme	Fish	103:106	arg1	oil					108:110	Fish oil	103:110	Fish oil	103:110	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	1	15	theme	Fish	103:106	arg1	source					127:132	the primary source	115:132	the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits	115:288	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	9	16	theme	sardine	1554:1560	arg1	oils					1562:1565	sardine oils	1554:1565	sardine oils	1554:1565	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	8	17	theme	%	1355:1355	arg1	EE					1324:1325	EE	1324:1325	EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively	1324:1411	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	8	18	theme	highest	1214:1220	arg1	efficiency					1236:1245	the highest encapsulation efficiency	1210:1245	the highest encapsulation efficiency	1210:1245	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	8	19	theme	encapsulated	1298:1309	arg1	samples					1311:1317	all encapsulated samples	1294:1317	all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively	1294:1411	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	10	20	theme	shelf	1811:1815	arg1	life					1817:1820	the shelf life	1807:1820	the shelf life of it	1807:1826	It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control).					
34684694	8	21	theme	sand	1361:1364	arg1	smelt					1366:1370	sand smelt	1361:1370	sand smelt	1361:1370	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	0	22	theme	Encapsulation	40:52	arg1	Efficiency					54:63	Encapsulation Efficiency	40:63	Encapsulation Efficiency	40:63	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	5	23	theme	encapsulation	793:805	arg1	efficiency					807:816	the encapsulation efficiency	789:816	the encapsulation efficiency (EE)	789:821	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	23	theme	encapsulation	793:805	arg1	EE					819:820	EE	819:820	EE	819:820	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	9	24	theme	storage	1584:1590	arg1	days					1576:1579	35 days	1573:1579	35 days of storage	1573:1590	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	8	25	theme	gum	1263:1265	arg1	Arabic					1267:1272	the gum Arabic	1259:1272	the gum Arabic	1259:1272	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	5	26	theme	encapsulated	864:875	arg1	oils					877:880	encapsulated oils	864:880	encapsulated oils	864:880	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	8	27	theme	sardine	1387:1393	arg1	oil					1395:1397	sardine oil	1387:1397	sardine oil	1387:1397	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	7	28	theme	effective	1090:1098	arg1	protein					1057:1063	Whey protein	1052:1063	Whey protein	1052:1063	Whey protein was found to be the most effective encapsulated agent comparing to gum Arabic and maltodextrin.					
34684694	7	28	theme	effective	1090:1098	arg1	agent					1113:1117	the most effective encapsulated agent	1081:1117	the most effective encapsulated agent comparing to gum Arabic and maltodextrin	1081:1158	Whey protein was found to be the most effective encapsulated agent comparing to gum Arabic and maltodextrin.					
34684694	9	29	theme	oil	1434:1436	arg1	control					1447:1453	control	1447:1453	control	1447:1453	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	9	29	theme	oil	1434:1436	arg1	samples					1438:1444	Unencapsulated fish oil samples	1414:1444	Unencapsulated fish oil samples (control)	1414:1454	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	6	30	theme	protective	972:981	arg1	effect					983:988	a highly protective effect	963:988	a highly protective effect against the oxidative deterioration of the encapsulated oils	963:1049	All three encapsulating agents provided a highly protective effect against the oxidative deterioration of the encapsulated oils.					
34684694	11	31	theme	functional	2004:2013	arg1	foods					2015:2019	functional foods	2004:2019	functional foods	2004:2019	The results suggest that encapsulation of fish oil is beneficial for its oxidative stability and its uses in the production of functional foods.					
34684694	1	32	dep	prevention	218:227	arg1	the					214:216	the	214:216	the	214:216	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	4	33	dep	materials	653:661	arg1	maltodextrin					699:710	maltodextrin	699:710	maltodextrin	699:710	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	33	dep	materials	653:661	arg1	materials					653:661	three different wall materials	632:661	three different wall materials (whey protein, gum Arabic (AG), and maltodextrin)	632:711	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	33	dep	materials	653:661	arg1	AG					690:691	AG	690:691	AG	690:691	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	33	dep	materials	653:661	arg1	protein					669:675	whey protein	664:675	whey protein	664:675	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	33	dep	materials	653:661	arg1	Arabic					682:687	gum Arabic	678:687	gum Arabic (AG)	678:692	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	11	34	theme	oil	1924:1926	arg1	encapsulation					1902:1914	encapsulation	1902:1914	encapsulation of fish oil	1902:1926	The results suggest that encapsulation of fish oil is beneficial for its oxidative stability and its uses in the production of functional foods.					
34684694	11	35	dep	its	1974:1976	arg1	uses					1978:1981	uses	1978:1981	uses	1978:1981	The results suggest that encapsulation of fish oil is beneficial for its oxidative stability and its uses in the production of functional foods.					
34684694	4	36	theme	gum	678:680	arg1	materials					653:661	three different wall materials	632:661	three different wall materials (whey protein, gum Arabic (AG), and maltodextrin)	632:711	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	36	theme	gum	678:680	arg1	AG					690:691	AG	690:691	AG	690:691	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	36	theme	gum	678:680	arg1	Arabic					682:687	gum Arabic	678:687	gum Arabic (AG)	678:692	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	1	37	theme	disease	252:258	arg1	prevention					218:227	prevention	218:227	prevention	218:227	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	1	37	theme	disease	252:258	arg1	treatment					233:241	treatment	233:241	treatment	233:241	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	7	38	dep	gum	1132:1134	arg1	maltodextrin					1147:1158	maltodextrin	1147:1158	maltodextrin	1147:1158	Whey protein was found to be the most effective encapsulated agent comparing to gum Arabic and maltodextrin.					
34684694	7	38	dep	gum	1132:1134	arg1	Arabic					1136:1141	Arabic	1136:1141	Arabic	1136:1141	Whey protein was found to be the most effective encapsulated agent comparing to gum Arabic and maltodextrin.					
34684694	4	39	theme	conventional	716:727	arg1	spray-drying					729:740	conventional spray-drying	716:740	conventional spray-drying	716:740	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	1	40	theme	long-chain	137:146	arg1	nutrients					189:197	important nutrients	179:197	important nutrients that assist in the prevention and treatment of heart disease	179:258	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	1	40	theme	long-chain	137:146	arg1	acids					162:166	long-chain omega-3 fatty acids	137:166	long-chain omega-3 fatty acids	137:166	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	0	41	theme	Material	19:26	arg1	Type					28:31	Wall Material Type	14:31	Wall Material Type	14:31	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	9	42	theme	peroxide	1465:1472	arg1	values					1474:1479	peroxide values	1465:1479	peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils	1465:1565	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	9	42	theme	peroxide	1465:1472	arg1	PV					1482:1483	PV	1482:1483	PV	1482:1483	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	5	43	theme	oxidative	841:849	arg1	stability					851:859	oxidative stability	841:859	oxidative stability	841:859	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	1	44	theme	health	274:279	arg1	benefits					281:288	many health benefits	269:288	many health benefits	269:288	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	11	45	theme	fish	1919:1922	arg1	oil					1924:1926	fish oil	1919:1926	fish oil	1919:1926	The results suggest that encapsulation of fish oil is beneficial for its oxidative stability and its uses in the production of functional foods.					
34684694	6	46	theme	encapsulated	1033:1044	arg1	oils					1046:1049	the encapsulated oils	1029:1049	the encapsulated oils	1029:1049	All three encapsulating agents provided a highly protective effect against the oxidative deterioration of the encapsulated oils.					
34684694	10	47	theme	oil	1855:1857	arg1	control					1867:1873	control	1867:1873	control	1867:1873	It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control).					
34684694	10	47	theme	oil	1855:1857	arg1	sample					1859:1864	the untreated oil sample	1841:1864	the untreated oil sample (control)	1841:1874	It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control).					
34684694	8	48	theme	%	1335:1335	arg1	EE					1324:1325	EE	1324:1325	EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively	1324:1411	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	3	49	theme	material	409:416	arg1	composition					418:428	the wall material composition	400:428	the wall material composition	400:428	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	8	50	from	Arabic	1267:1272	arg1	samples					1311:1317	all encapsulated samples	1294:1317	all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively	1294:1411	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	1	51	theme	primary	119:125	arg1	oil					108:110	Fish oil	103:110	Fish oil	103:110	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	1	51	theme	primary	119:125	arg1	source					127:132	the primary source	115:132	the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits	115:288	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	9	52	theme	same	1658:1661	arg1	period					1671:1676	the same storage period	1654:1676	the same storage period	1654:1676	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	2	53	dep	vitamins	349:356	arg1	vitamins					349:356	vitamins	349:356	vitamins A and D	349:364	It also contains vitamins that are lipid-soluble, such as vitamins A and D.					
34684694	2	53	dep	vitamins	349:356	arg1	D					364:364	D	364:364	D	364:364	It also contains vitamins that are lipid-soluble, such as vitamins A and D.					
34684694	2	53	dep	vitamins	349:356	arg1	A					358:358	A	358:358	A	358:358	It also contains vitamins that are lipid-soluble, such as vitamins A and D.					
34684694	5	54	theme	wall	771:774	arg1	materials					776:784	the different wall materials	757:784	the different wall materials	757:784	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	6	55	theme	oxidative	1002:1010	arg1	deterioration					1012:1024	the oxidative deterioration	998:1024	the oxidative deterioration of the encapsulated oils	998:1049	All three encapsulating agents provided a highly protective effect against the oxidative deterioration of the encapsulated oils.					
34684694	3	56	theme	encapsulation	445:457	arg1	efficiency					459:468	encapsulation efficiency	445:468	encapsulation efficiency	445:468	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	3	57	from	efficiency	459:468	arg1	microcapsules					528:540	spray-dried microcapsules	516:540	spray-dried microcapsules	516:540	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	3	58	theme	omega	497:501	arg1	oils					508:511	omega fish oils	497:511	omega fish oils	497:511	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	8	59	from	maltodextrin	1278:1289	arg1	samples					1311:1317	all encapsulated samples	1294:1317	all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively	1294:1411	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	5	60	from	°C	902:903	arg1	efficiency					807:816	the encapsulation efficiency	789:816	the encapsulation efficiency (EE)	789:821	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	60	from	°C	902:903	arg1	stability					851:859	oxidative stability	841:859	oxidative stability	841:859	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	60	from	°C	902:903	arg1	EE					819:820	EE	819:820	EE	819:820	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	60	from	°C	902:903	arg1	flowability					824:834	flowability	824:834	flowability	824:834	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	61	theme	different	761:769	arg1	materials					776:784	the different wall materials	757:784	the different wall materials	757:784	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	7	62	theme	Whey	1052:1055	arg1	protein					1057:1063	Whey protein	1052:1063	Whey protein	1052:1063	Whey protein was found to be the most effective encapsulated agent comparing to gum Arabic and maltodextrin.					
34684694	7	62	theme	Whey	1052:1055	arg1	agent					1113:1117	the most effective encapsulated agent	1081:1117	the most effective encapsulated agent comparing to gum Arabic and maltodextrin	1081:1158	Whey protein was found to be the most effective encapsulated agent comparing to gum Arabic and maltodextrin.					
34684694	9	63	theme	meq/kg	1513:1518	arg1	oil					1520:1522	33.19, 40.64, and 47.76 meq/kg oil	1489:1522	33.19, 40.64, and 47.76 meq/kg oil	1489:1522	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	4	64	theme	sand	591:594	arg1	oil					607:609	sand smelt fish oil	591:609	sand smelt fish oil	591:609	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	3	65	theme	spray-dried	516:526	arg1	microcapsules					528:540	spray-dried microcapsules	516:540	spray-dried microcapsules	516:540	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	5	66	theme	materials	776:784	arg1	effect					747:752	The effect	743:752	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C	743:903	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	4	67	theme	fish	602:605	arg1	oil					607:609	sand smelt fish oil	591:609	sand smelt fish oil	591:609	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	3	68	theme	oils	508:511	arg1	efficiency					459:468	encapsulation efficiency	445:468	encapsulation efficiency	445:468	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	3	68	theme	oils	508:511	arg1	stability					484:492	oxidative stability	474:492	oxidative stability	474:492	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	1	69	theme	fatty	156:160	arg1	nutrients					189:197	important nutrients	179:197	important nutrients that assist in the prevention and treatment of heart disease	179:258	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	1	69	theme	fatty	156:160	arg1	acids					162:166	long-chain omega-3 fatty acids	137:166	long-chain omega-3 fatty acids	137:166	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	4	70	theme	waste	576:580	arg1	oil					582:584	sardine waste oil	568:584	sardine waste oil	568:584	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	8	71	with	samples	1311:1317	arg1	EE					1324:1325	EE	1324:1325	EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively	1324:1411	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	10	72	theme	fish	1784:1787	arg1	oil					1789:1791	the encapsulated fish oil	1767:1791	the encapsulated fish oil	1767:1791	It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control).					
34684694	4	73	theme	different	638:646	arg1	maltodextrin					699:710	maltodextrin	699:710	maltodextrin	699:710	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	73	theme	different	638:646	arg1	materials					653:661	three different wall materials	632:661	three different wall materials (whey protein, gum Arabic (AG), and maltodextrin)	632:711	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	73	theme	different	638:646	arg1	protein					669:675	whey protein	664:675	whey protein	664:675	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	73	theme	different	638:646	arg1	Arabic					682:687	gum Arabic	678:687	gum Arabic (AG)	678:692	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	5	74	from	effect	747:752	arg1	efficiency					807:816	the encapsulation efficiency	789:816	the encapsulation efficiency (EE)	789:821	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	74	from	effect	747:752	arg1	stability					851:859	oxidative stability	841:859	oxidative stability	841:859	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	74	from	effect	747:752	arg1	EE					819:820	EE	819:820	EE	819:820	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	74	from	effect	747:752	arg1	flowability					824:834	flowability	824:834	flowability	824:834	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	8	75	theme	mackerel	1373:1380	arg1	EE					1324:1325	EE	1324:1325	EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively	1324:1411	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	8	76	theme	encapsulation	1222:1234	arg1	efficiency					1236:1245	the highest encapsulation efficiency	1210:1245	the highest encapsulation efficiency	1210:1245	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	3	77	from	stability	484:492	arg1	microcapsules					528:540	spray-dried microcapsules	516:540	spray-dried microcapsules	516:540	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	0	78	theme	Oxidative	69:77	arg1	Stability					79:87	Oxidative Stability	69:87	Oxidative Stability	69:87	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	0	79	from	Effect	4:9	arg1	Efficiency					54:63	Encapsulation Efficiency	40:63	Encapsulation Efficiency	40:63	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	0	79	from	Effect	4:9	arg1	Stability					79:87	Oxidative Stability	69:87	Oxidative Stability	69:87	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	10	80	theme	it	1825:1826	arg1	life					1817:1820	the shelf life	1807:1820	the shelf life of it	1807:1826	It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control).					
34684694	7	81	theme	encapsulated	1100:1111	arg1	protein					1057:1063	Whey protein	1052:1063	Whey protein	1052:1063	Whey protein was found to be the most effective encapsulated agent comparing to gum Arabic and maltodextrin.					
34684694	7	81	theme	encapsulated	1100:1111	arg1	agent					1113:1117	the most effective encapsulated agent	1081:1117	the most effective encapsulated agent comparing to gum Arabic and maltodextrin	1081:1158	Whey protein was found to be the most effective encapsulated agent comparing to gum Arabic and maltodextrin.					
34684694	2	82	contain	contains	299:306	arg1	It					291:292	It	291:292	It	291:292	It also contains vitamins that are lipid-soluble, such as vitamins A and D.					
34684694	2	82	contain	contains	299:306	arg2	vitamins					308:315	vitamins	308:315	vitamins that are lipid-soluble, such as vitamins A and D	308:364	It also contains vitamins that are lipid-soluble, such as vitamins A and D.					
34684694	11	83	theme	foods	2015:2019	arg1	production					1990:1999	the production	1986:1999	the production of functional foods	1986:2019	The results suggest that encapsulation of fish oil is beneficial for its oxidative stability and its uses in the production of functional foods.					
34684694	8	84	theme	oil	1395:1397	arg1	EE					1324:1325	EE	1324:1325	EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively	1324:1411	The results indicated that whey protein recorded the highest encapsulation efficiency compared to the gum Arabic and maltodextrin in all encapsulated samples with EE of 71.71%, 68.61%, and 64.71% for sand smelt, mackerel, and sardine oil, respectively.					
34684694	9	85	theme	Unencapsulated	1414:1427	arg1	control					1447:1453	control	1447:1453	control	1447:1453	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	9	85	theme	Unencapsulated	1414:1427	arg1	samples					1438:1444	Unencapsulated fish oil samples	1414:1444	Unencapsulated fish oil samples (control)	1414:1454	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	0	86	theme	Oils	97:100	arg1	Efficiency					54:63	Encapsulation Efficiency	40:63	Encapsulation Efficiency	40:63	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	0	86	theme	Oils	97:100	arg1	Stability					79:87	Oxidative Stability	69:87	Oxidative Stability	69:87	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	5	87	theme	oils	877:880	arg1	efficiency					807:816	the encapsulation efficiency	789:816	the encapsulation efficiency (EE)	789:821	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	87	theme	oils	877:880	arg1	stability					851:859	oxidative stability	841:859	oxidative stability	841:859	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	87	theme	oils	877:880	arg1	EE					819:820	EE	819:820	EE	819:820	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	5	87	theme	oils	877:880	arg1	flowability					824:834	flowability	824:834	flowability	824:834	The effect of the different wall materials on the encapsulation efficiency (EE), flowability, and oxidative stability of encapsulated oils during storage at 4 °C was investigated.					
34684694	9	88	theme	encapsulated	1607:1618	arg1	samples					1620:1626	all the encapsulated samples	1599:1626	all the encapsulated samples	1599:1626	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	4	89	theme	whey	664:667	arg1	materials					653:661	three different wall materials	632:661	three different wall materials (whey protein, gum Arabic (AG), and maltodextrin)	632:711	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	4	89	theme	whey	664:667	arg1	protein					669:675	whey protein	664:675	whey protein	664:675	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	0	90	theme	Wall	14:17	arg1	Type					28:31	Wall Material Type	14:31	Wall Material Type	14:31	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	1	91	theme	heart	246:250	arg1	disease					252:258	heart disease	246:258	heart disease	246:258	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	0	92	dep	Efficiency	54:63	arg1	the					36:38	the	36:38	the	36:38	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	9	93	theme	fish	1429:1432	arg1	control					1447:1453	control	1447:1453	control	1447:1453	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	9	93	theme	fish	1429:1432	arg1	samples					1438:1444	Unencapsulated fish oil samples	1414:1444	Unencapsulated fish oil samples (control)	1414:1454	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	3	94	theme	wall	404:407	arg1	composition					418:428	the wall material composition	400:428	the wall material composition	400:428	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34684694	0	95	theme	Type	28:31	arg1	Effect					4:9	The Effect	0:9	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.	0:101	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	6	96	theme	encapsulating	933:945	arg1	agents					947:952	All three encapsulating agents	923:952	All three encapsulating agents	923:952	All three encapsulating agents provided a highly protective effect against the oxidative deterioration of the encapsulated oils.					
34684694	0	97	theme	Fish	92:95	arg1	Oils					97:100	Fish Oils	92:100	Fish Oils	92:100	The Effect of Wall Material Type on the Encapsulation Efficiency and Oxidative Stability of Fish Oils.					
34684694	1	98	theme	many	269:272	arg1	benefits					281:288	many health benefits	269:288	many health benefits	269:288	Fish oil is the primary source of long-chain omega-3 fatty acids, which are important nutrients that assist in the prevention and treatment of heart disease and have many health benefits.					
34684694	6	99	theme	oils	1046:1049	arg1	deterioration					1012:1024	the oxidative deterioration	998:1024	the oxidative deterioration of the encapsulated oils	998:1049	All three encapsulating agents provided a highly protective effect against the oxidative deterioration of the encapsulated oils.					
34684694	11	100	theme	oxidative	1950:1958	arg1	stability					1960:1968	its oxidative stability	1946:1968	its oxidative stability	1946:1968	The results suggest that encapsulation of fish oil is beneficial for its oxidative stability and its uses in the production of functional foods.					
34684694	10	101	theme	untreated	1845:1853	arg1	control					1867:1873	control	1867:1873	control	1867:1873	It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control).					
34684694	10	101	theme	untreated	1845:1853	arg1	sample					1859:1864	the untreated oil sample	1841:1864	the untreated oil sample (control)	1841:1874	It could be concluded that all the encapsulating agents provided a protective effect to the encapsulated fish oil and elongated the shelf life of it comparing to the untreated oil sample (control).					
34684694	9	102	theme	storage	1663:1669	arg1	period					1671:1676	the same storage period	1654:1676	the same storage period	1654:1676	Unencapsulated fish oil samples (control) recorded peroxide values (PV) of 33.19, 40.64, and 47.76 meq/kg oil for sand smelt, mackerel, and sardine oils after 35 days of storage, while all the encapsulated samples showed PV less than 10 in the same storage period.					
34684694	4	103	theme	sardine	568:574	arg1	oil					582:584	sardine waste oil	568:584	sardine waste oil	568:584	In this study, mackerel, sardine waste oil, and sand smelt fish oil were encapsulated in three different wall materials (whey protein, gum Arabic (AG), and maltodextrin) by conventional spray-drying.					
34684694	3	104	theme	oxidative	474:482	arg1	stability					484:492	oxidative stability	474:492	oxidative stability	474:492	This work aimed to determine how the wall material composition influenced the encapsulation efficiency and oxidative stability of omega fish oils in spray-dried microcapsules.					
34673108	8	0	theme	enthalpy	1147:1154	arg1	change					1156:1161	enthalpy change	1147:1161	enthalpy change	1147:1161	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	5	1	from	N-acetyl-D-galactosamine	811:834	arg1	ratio					855:859	a ratio	853:859	a ratio of 5.2:2.5:6.4:1.0	853:878	The monosaccharide analysis established its composition to be glucose, galactose, N-acetyl-D-galactosamine, and rhamnose in a ratio of 5.2:2.5:6.4:1.0.					
34673108	10	2	theme	promising	1409:1417	arg1	properties					1442:1451	promising in vitro antibacterial properties	1409:1451	promising in vitro antibacterial properties	1409:1451	Furthermore, it also demonstrated similar antioxidant activity to XG and promising in vitro antibacterial properties.					
34673108	6	3	theme	techniques	1006:1015	arg1	combination					937:947	the combination	933:947	the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques	933:1015	The molecular structure of EPS-3A was determined by the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques.					
34673108	4	4	theme	weight-average	589:602	arg1	Mw					622:623	Mw	622:623	Mw	622:623	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	4	4	theme	weight-average	589:602	arg1	weight					614:619	The weight-average molecular weight	585:619	The weight-average molecular weight (Mw) of EPS-3A	585:634	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	4	4	theme	weight-average	589:602	arg1	1.38 × 106 Da					656:668	1.38 × 106 Da	656:668	1.38 × 106 Da	656:668	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	11	5	attach	derived	1486:1492	arg1	ZJUIDS-2-01					1515:1525	S. thermophilus ZJUIDS-2-01	1499:1525	S. thermophilus ZJUIDS-2-01	1499:1525	This work evidenced that EPS-3A derived from S. thermophilus ZJUIDS-2-01 holds the potential for food and industrial applications.					
34673108	11	5	attach	derived	1486:1492	arg2	EPS-3A					1479:1484	EPS-3A	1479:1484	EPS-3A derived from S. thermophilus ZJUIDS-2-01	1479:1525	This work evidenced that EPS-3A derived from S. thermophilus ZJUIDS-2-01 holds the potential for food and industrial applications.					
34673108	5	6	theme	monosaccharide	733:746	arg1	analysis					748:755	The monosaccharide analysis	729:755	The monosaccharide analysis	729:755	The monosaccharide analysis established its composition to be glucose, galactose, N-acetyl-D-galactosamine, and rhamnose in a ratio of 5.2:2.5:6.4:1.0.					
34673108	5	7	from	galactose	800:808	arg1	ratio					855:859	a ratio	853:859	a ratio of 5.2:2.5:6.4:1.0	853:878	The monosaccharide analysis established its composition to be glucose, galactose, N-acetyl-D-galactosamine, and rhamnose in a ratio of 5.2:2.5:6.4:1.0.					
34673108	6	8	theme	molecular	885:893	arg1	structure					895:903	The molecular structure	881:903	The molecular structure of EPS-3A	881:913	The molecular structure of EPS-3A was determined by the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques.					
34673108	8	9	theme	change	1156:1161	arg1	Tm					1128:1129	Tm	1128:1129	Tm	1128:1129	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	8	9	theme	change	1156:1161	arg1	point					1121:1125	a melting point	1111:1125	a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS	1111:1251	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	5	10	from	rhamnose	841:848	arg1	ratio					855:859	a ratio	853:859	a ratio of 5.2:2.5:6.4:1.0	853:878	The monosaccharide analysis established its composition to be glucose, galactose, N-acetyl-D-galactosamine, and rhamnose in a ratio of 5.2:2.5:6.4:1.0.					
34673108	9	11	theme	emulsion	1282:1289	arg1	stability					1291:1299	better O/W emulsion stability	1271:1299	better O/W emulsion stability	1271:1299	EPS-3A exhibited better O/W emulsion stability and flocculating capacity than XG.					
34673108	11	12	theme	S.	1499:1500	arg1	ZJUIDS-2-01					1515:1525	S. thermophilus ZJUIDS-2-01	1499:1525	S. thermophilus ZJUIDS-2-01	1499:1525	This work evidenced that EPS-3A derived from S. thermophilus ZJUIDS-2-01 holds the potential for food and industrial applications.					
34673108	1	13	theme	diverse	265:271	arg1	array					273:277	a diverse array	263:277	a diverse array of microorganisms	263:295	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	0	14	theme	yak	127:129	arg1	yogurt					131:136	traditional yak yogurt	115:136	traditional yak yogurt	115:136	Characterization of an exopolysaccharide (EPS-3A) produced by Streptococcus thermophilus ZJUIDS-2-01 isolated from traditional yak yogurt.					
34673108	3	15	theme	bioactivity	491:501	arg1	studies					503:509	detailed structural, physiochemical, and bioactivity studies	450:509	detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01	450:582	We further purified and carried out detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01.					
34673108	4	16	theme	EPS-3A	629:634	arg1	Mw					622:623	Mw	622:623	Mw	622:623	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	4	16	theme	EPS-3A	629:634	arg1	weight					614:619	The weight-average molecular weight	585:619	The weight-average molecular weight (Mw) of EPS-3A	585:634	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	4	16	theme	EPS-3A	629:634	arg1	1.38 × 106 Da					656:668	1.38 × 106 Da	656:668	1.38 × 106 Da	656:668	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	4	17	theme	High-Performance	673:688	arg1	Chromatography					705:718	High-Performance Gel Permeation Chromatography	673:718	High-Performance Gel Permeation Chromatography (HPGPC)	673:726	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	4	17	theme	High-Performance	673:688	arg1	HPGPC					721:725	HPGPC	721:725	HPGPC	721:725	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	2	18	theme	yak	402:404	arg1	yogurt					406:411	the traditional yak yogurt	386:411	the traditional yak yogurt	386:411	We isolated and identified a novel Streptococcus thermophilus strain, ZJUIDS-2-01, from the traditional yak yogurt.					
34673108	0	19	theme	traditional	115:125	arg1	yogurt					131:136	traditional yak yogurt	115:136	traditional yak yogurt	115:136	Characterization of an exopolysaccharide (EPS-3A) produced by Streptococcus thermophilus ZJUIDS-2-01 isolated from traditional yak yogurt.					
34673108	6	20	theme	EPS-3A	908:913	arg1	structure					895:903	The molecular structure	881:903	The molecular structure of EPS-3A	881:913	The molecular structure of EPS-3A was determined by the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques.					
34673108	10	21	theme	antibacterial	1428:1440	arg1	properties					1442:1451	promising in vitro antibacterial properties	1409:1451	promising in vitro antibacterial properties	1409:1451	Furthermore, it also demonstrated similar antioxidant activity to XG and promising in vitro antibacterial properties.					
34673108	2	22	theme	traditional	390:400	arg1	yogurt					406:411	the traditional yak yogurt	386:411	the traditional yak yogurt	386:411	We isolated and identified a novel Streptococcus thermophilus strain, ZJUIDS-2-01, from the traditional yak yogurt.					
34673108	4	23	theme	molecular	604:612	arg1	Mw					622:623	Mw	622:623	Mw	622:623	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	4	23	theme	molecular	604:612	arg1	weight					614:619	The weight-average molecular weight	585:619	The weight-average molecular weight (Mw) of EPS-3A	585:634	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	4	23	theme	molecular	604:612	arg1	1.38 × 106 Da					656:668	1.38 × 106 Da	656:668	1.38 × 106 Da	656:668	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	9	24	theme	O/W	1278:1280	arg1	stability					1291:1299	better O/W emulsion stability	1271:1299	better O/W emulsion stability	1271:1299	EPS-3A exhibited better O/W emulsion stability and flocculating capacity than XG.					
34673108	8	25	theme	80.4 °C	1135:1141	arg1	Tm					1128:1129	Tm	1128:1129	Tm	1128:1129	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	8	25	theme	80.4 °C	1135:1141	arg1	point					1121:1125	a melting point	1111:1125	a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS	1111:1251	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	1	26	theme	dairy	182:186	arg1	products					188:195	the naturally fermented dairy products	158:195	the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau	158:251	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	10	27	dep	in	1419:1420	arg1	vitro					1422:1426	vitro	1422:1426	vitro	1422:1426	Furthermore, it also demonstrated similar antioxidant activity to XG and promising in vitro antibacterial properties.					
34673108	6	28	theme	spectroscopic	992:1004	arg1	techniques					1006:1015	NMR spectroscopic techniques	988:1015	NMR spectroscopic techniques	988:1015	The molecular structure of EPS-3A was determined by the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques.					
34673108	10	29	theme	in	1419:1420	arg1	properties					1442:1451	promising in vitro antibacterial properties	1409:1451	promising in vitro antibacterial properties	1409:1451	Furthermore, it also demonstrated similar antioxidant activity to XG and promising in vitro antibacterial properties.					
34673108	10	30	theme	similar	1370:1376	arg1	activity					1390:1397	similar antioxidant activity	1370:1397	similar antioxidant activity	1370:1397	Furthermore, it also demonstrated similar antioxidant activity to XG and promising in vitro antibacterial properties.					
34673108	1	31	theme	products	188:195	arg1	one					151:153	one	151:153	one	151:153	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	1	31	theme	products	188:195	arg1	products					188:195	the naturally fermented dairy products	158:195	the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau	158:251	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	1	31	theme	products	188:195	arg1	yogurt					143:148	Yak yogurt	139:148	Yak yogurt	139:148	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	0	32	theme	exopolysaccharide	23:39	arg1	Characterization					0:15	Characterization	0:15	Characterization of an exopolysaccharide (EPS-3A) produced by Streptococcus thermophilus ZJUIDS-2-01	0:99	Characterization of an exopolysaccharide (EPS-3A) produced by Streptococcus thermophilus ZJUIDS-2-01 isolated from traditional yak yogurt.					
34673108	6	33	theme	NMR	988:990	arg1	techniques					1006:1015	NMR spectroscopic techniques	988:1015	NMR spectroscopic techniques	988:1015	The molecular structure of EPS-3A was determined by the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques.					
34673108	11	34	theme	thermophilus	1502:1513	arg1	ZJUIDS-2-01					1515:1525	S. thermophilus ZJUIDS-2-01	1499:1525	S. thermophilus ZJUIDS-2-01	1499:1525	This work evidenced that EPS-3A derived from S. thermophilus ZJUIDS-2-01 holds the potential for food and industrial applications.					
34673108	3	35	theme	S.	556:557	arg1	ZJUIDS-2-01					572:582	S. thermophilus ZJUIDS-2-01	556:582	S. thermophilus ZJUIDS-2-01	556:582	We further purified and carried out detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01.					
34673108	7	36	theme	ζ-potential	1022:1032	arg1	measurements					1034:1045	The ζ-potential measurements	1018:1045	The ζ-potential measurements	1018:1045	The ζ-potential measurements indicated that EPS-3A had a pKa value of ~4.40.					
34673108	1	37	theme	microorganisms	282:295	arg1	array					273:277	a diverse array	263:277	a diverse array of microorganisms	263:295	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	3	38	theme	thermophilus	559:570	arg1	ZJUIDS-2-01					572:582	S. thermophilus ZJUIDS-2-01	556:582	S. thermophilus ZJUIDS-2-01	556:582	We further purified and carried out detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01.					
34673108	3	39	theme	physiochemical	471:484	arg1	studies					503:509	detailed structural, physiochemical, and bioactivity studies	450:509	detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01	450:582	We further purified and carried out detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01.					
34673108	8	40	theme	comparable	1191:1200	arg1	EPS-3A					1183:1188	EPS-3A	1183:1188	EPS-3A	1183:1188	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	7	41	contain	had	1069:1071	arg2	value					1079:1083	a pKa value	1073:1083	a pKa value of ~4.40	1073:1092	The ζ-potential measurements indicated that EPS-3A had a pKa value of ~4.40.					
34673108	7	41	contain	had	1069:1071	arg1	EPS-3A					1062:1067	EPS-3A	1062:1067	EPS-3A	1062:1067	The ζ-potential measurements indicated that EPS-3A had a pKa value of ~4.40.					
34673108	8	42	theme	melting	1113:1119	arg1	Tm					1128:1129	Tm	1128:1129	Tm	1128:1129	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	8	42	theme	melting	1113:1119	arg1	point					1121:1125	a melting point	1111:1125	a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS	1111:1251	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	3	43	theme	detailed	450:457	arg1	studies					503:509	detailed structural, physiochemical, and bioactivity studies	450:509	detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01	450:582	We further purified and carried out detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01.					
34673108	8	44	theme	578 J/g	1171:1177	arg1	ΔH					1164:1165	ΔH	1164:1165	ΔH	1164:1165	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	8	44	theme	578 J/g	1171:1177	arg1	80.4 °C					1135:1141	80.4 °C	1135:1141	80.4 °C	1135:1141	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	8	44	theme	578 J/g	1171:1177	arg1	change					1156:1161	enthalpy change	1147:1161	enthalpy change	1147:1161	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	8	45	theme	xanthan	1218:1224	arg1	EPS					1249:1251	a commercial EPS	1236:1251	a commercial EPS	1236:1251	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	8	45	theme	xanthan	1218:1224	arg1	XG					1231:1232	XG	1231:1232	XG	1231:1232	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	8	45	theme	xanthan	1218:1224	arg1	gum					1226:1228	the xanthan gum	1214:1228	the xanthan gum (XG)	1214:1233	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	1	46	theme	local	209:213	arg1	herdsmen					215:222	local herdsmen	209:222	local herdsmen in the Qinghai-Tibet Plateau	209:251	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	4	47	theme	Permeation	694:703	arg1	Chromatography					705:718	High-Performance Gel Permeation Chromatography	673:718	High-Performance Gel Permeation Chromatography (HPGPC)	673:726	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	4	47	theme	Permeation	694:703	arg1	HPGPC					721:725	HPGPC	721:725	HPGPC	721:725	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	6	48	theme	FT-IR	977:981	arg1	combination					937:947	the combination	933:947	the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques	933:1015	The molecular structure of EPS-3A was determined by the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques.					
34673108	7	49	theme	~4.40	1088:1092	arg1	value					1079:1083	a pKa value	1073:1083	a pKa value of ~4.40	1073:1092	The ζ-potential measurements indicated that EPS-3A had a pKa value of ~4.40.					
34673108	5	50	theme	5.2:2.5:6.4:1.0	864:878	arg1	ratio					855:859	a ratio	853:859	a ratio of 5.2:2.5:6.4:1.0	853:878	The monosaccharide analysis established its composition to be glucose, galactose, N-acetyl-D-galactosamine, and rhamnose in a ratio of 5.2:2.5:6.4:1.0.					
34673108	3	51	theme	structural	459:468	arg1	studies					503:509	detailed structural, physiochemical, and bioactivity studies	450:509	detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01	450:582	We further purified and carried out detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01.					
34673108	8	52	theme	commercial	1238:1247	arg1	EPS					1249:1251	a commercial EPS	1236:1251	a commercial EPS	1236:1251	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	8	52	theme	commercial	1238:1247	arg1	gum					1226:1228	the xanthan gum	1214:1228	the xanthan gum (XG)	1214:1233	The DSC yielded a melting point (Tm) of 80.4 °C and enthalpy change (ΔH) of 578 J/g for EPS-3A, comparable to those of the xanthan gum (XG), a commercial EPS.					
34673108	7	53	theme	pKa	1075:1077	arg1	value					1079:1083	a pKa value	1073:1083	a pKa value of ~4.40	1073:1092	The ζ-potential measurements indicated that EPS-3A had a pKa value of ~4.40.					
34673108	1	54	from	herdsmen	215:222	arg1	Plateau					245:251	the Qinghai-Tibet Plateau	227:251	the Qinghai-Tibet Plateau	227:251	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	2	55	theme	thermophilus	347:358	arg1	ZJUIDS-2-01					368:378	ZJUIDS-2-01	368:378	ZJUIDS-2-01	368:378	We isolated and identified a novel Streptococcus thermophilus strain, ZJUIDS-2-01, from the traditional yak yogurt.					
34673108	2	55	theme	thermophilus	347:358	arg1	strain					360:365	a novel Streptococcus thermophilus strain	325:365	a novel Streptococcus thermophilus strain	325:365	We isolated and identified a novel Streptococcus thermophilus strain, ZJUIDS-2-01, from the traditional yak yogurt.					
34673108	6	56	theme	analysis	967:974	arg1	combination					937:947	the combination	933:947	the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques	933:1015	The molecular structure of EPS-3A was determined by the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques.					
34673108	2	57	theme	Streptococcus	333:345	arg1	ZJUIDS-2-01					368:378	ZJUIDS-2-01	368:378	ZJUIDS-2-01	368:378	We isolated and identified a novel Streptococcus thermophilus strain, ZJUIDS-2-01, from the traditional yak yogurt.					
34673108	2	57	theme	Streptococcus	333:345	arg1	strain					360:365	a novel Streptococcus thermophilus strain	325:365	a novel Streptococcus thermophilus strain	325:365	We isolated and identified a novel Streptococcus thermophilus strain, ZJUIDS-2-01, from the traditional yak yogurt.					
34673108	6	58	theme	permethylation	952:965	arg1	analysis					967:974	permethylation analysis	952:974	permethylation analysis	952:974	The molecular structure of EPS-3A was determined by the combination of permethylation analysis, FT-IR, and NMR spectroscopic techniques.					
34673108	5	59	from	glucose	791:797	arg1	ratio					855:859	a ratio	853:859	a ratio of 5.2:2.5:6.4:1.0	853:878	The monosaccharide analysis established its composition to be glucose, galactose, N-acetyl-D-galactosamine, and rhamnose in a ratio of 5.2:2.5:6.4:1.0.					
34673108	9	60	theme	flocculating	1305:1316	arg1	capacity					1318:1325	flocculating capacity	1305:1325	flocculating capacity	1305:1325	EPS-3A exhibited better O/W emulsion stability and flocculating capacity than XG.					
34673108	11	61	theme	industrial	1560:1569	arg1	applications					1571:1582	industrial applications	1560:1582	industrial applications	1560:1582	This work evidenced that EPS-3A derived from S. thermophilus ZJUIDS-2-01 holds the potential for food and industrial applications.					
34673108	1	62	theme	Yak	139:141	arg1	one					151:153	one	151:153	one	151:153	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	1	62	theme	Yak	139:141	arg1	yogurt					143:148	Yak yogurt	139:148	Yak yogurt	139:148	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	1	62	theme	Yak	139:141	arg1	products					188:195	the naturally fermented dairy products	158:195	the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau	158:251	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	2	63	theme	novel	327:331	arg1	ZJUIDS-2-01					368:378	ZJUIDS-2-01	368:378	ZJUIDS-2-01	368:378	We isolated and identified a novel Streptococcus thermophilus strain, ZJUIDS-2-01, from the traditional yak yogurt.					
34673108	2	63	theme	novel	327:331	arg1	strain					360:365	a novel Streptococcus thermophilus strain	325:365	a novel Streptococcus thermophilus strain	325:365	We isolated and identified a novel Streptococcus thermophilus strain, ZJUIDS-2-01, from the traditional yak yogurt.					
34673108	0	64	theme	Streptococcus	62:74	arg1	thermophilus					76:87	Streptococcus thermophilus ZJUIDS-2-01	62:99	Streptococcus thermophilus ZJUIDS-2-01	62:99	Characterization of an exopolysaccharide (EPS-3A) produced by Streptococcus thermophilus ZJUIDS-2-01 isolated from traditional yak yogurt.					
34673108	3	65	contain	carried	438:444	arg2	studies					503:509	detailed structural, physiochemical, and bioactivity studies	450:509	detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01	450:582	We further purified and carried out detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01.					
34673108	3	65	contain	carried	438:444	arg1	We					414:415	We	414:415	We	414:415	We further purified and carried out detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01.					
34673108	1	66	contain	contains	254:261	arg2	array					273:277	a diverse array	263:277	a diverse array of microorganisms	263:295	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	1	66	contain	contains	254:261	arg1	yogurt					143:148	Yak yogurt	139:148	Yak yogurt	139:148	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	1	66	contain	contains	254:261	arg1	products					188:195	the naturally fermented dairy products	158:195	the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau	158:251	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	1	66	contain	contains	254:261	arg1	one					151:153	one	151:153	one	151:153	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	2	67	from	yogurt	406:411	arg1	ZJUIDS-2-01					368:378	ZJUIDS-2-01	368:378	ZJUIDS-2-01	368:378	We isolated and identified a novel Streptococcus thermophilus strain, ZJUIDS-2-01, from the traditional yak yogurt.					
34673108	2	67	from	yogurt	406:411	arg1	strain					360:365	a novel Streptococcus thermophilus strain	325:365	a novel Streptococcus thermophilus strain	325:365	We isolated and identified a novel Streptococcus thermophilus strain, ZJUIDS-2-01, from the traditional yak yogurt.					
34673108	3	68	theme	exopolysaccharide	517:533	arg1	studies					503:509	detailed structural, physiochemical, and bioactivity studies	450:509	detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01	450:582	We further purified and carried out detailed structural, physiochemical, and bioactivity studies of an exopolysaccharide (EPS-3A) produced by S. thermophilus ZJUIDS-2-01.					
34673108	1	69	theme	fermented	172:180	arg1	products					188:195	the naturally fermented dairy products	158:195	the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau	158:251	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	1	70	theme	Qinghai-Tibet	231:243	arg1	Plateau					245:251	the Qinghai-Tibet Plateau	227:251	the Qinghai-Tibet Plateau	227:251	Yak yogurt, one of the naturally fermented dairy products prepared by local herdsmen in the Qinghai-Tibet Plateau, contains a diverse array of microorganisms.					
34673108	10	71	theme	antioxidant	1378:1388	arg1	activity					1390:1397	similar antioxidant activity	1370:1397	similar antioxidant activity	1370:1397	Furthermore, it also demonstrated similar antioxidant activity to XG and promising in vitro antibacterial properties.					
34673108	9	72	theme	better	1271:1276	arg1	stability					1291:1299	better O/W emulsion stability	1271:1299	better O/W emulsion stability	1271:1299	EPS-3A exhibited better O/W emulsion stability and flocculating capacity than XG.					
34673108	4	73	theme	Gel	690:692	arg1	Chromatography					705:718	High-Performance Gel Permeation Chromatography	673:718	High-Performance Gel Permeation Chromatography (HPGPC)	673:726	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34673108	4	73	theme	Gel	690:692	arg1	HPGPC					721:725	HPGPC	721:725	HPGPC	721:725	The weight-average molecular weight (Mw) of EPS-3A was estimated to be 1.38 × 106 Da by High-Performance Gel Permeation Chromatography (HPGPC).					
34537292	4	0	theme	uniform	713:719	arg1	weight					731:736	uniform molecular weight	713:736	uniform molecular weight	713:736	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	5	1	used	used	811:814	arg2	CA					804:805	CA	804:805	CA	804:805	Moreover, CA was used to reinforce polylactic acid (PLA) films prepared by solvent casting.					
34537292	0	2	from	separation	10:19	arg1	strength					99:106	the mechanical strength	84:106	the mechanical strength of polylactic acid	84:125	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	6	3	theme	%	979:979	arg1	increase					931:938	an increase	928:938	an increase of 80.63% in tensile strength and 59.51% in Young's modulus	928:998	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	6	4	theme	5 wt	912:915	arg1	CA					918:919	5 wt% CA	912:919	5 wt% CA	912:919	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	7	5	theme	excellent	1084:1092	arg1	properties					1105:1114	The lightweight and excellent mechanical properties	1064:1114	The lightweight and excellent mechanical properties	1064:1114	The lightweight and excellent mechanical properties promote the application potential of biodegradable composites to replace petroleum-based plastics.					
34537292	2	6	theme	eucalyptus	278:287	arg1	powder					289:294	the directly acetylated eucalyptus powder	254:294	the directly acetylated eucalyptus powder	254:294	Cellulose acetate (CA) was isolated from the directly acetylated eucalyptus powder after hydrothermal treatment to selectively remove hemicellulose without delignification.					
34537292	0	7	theme	mechanical	88:97	arg1	strength					99:106	the mechanical strength	84:106	the mechanical strength of polylactic acid	84:125	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	7	8	theme	mechanical	1094:1103	arg1	properties					1105:1114	The lightweight and excellent mechanical properties	1064:1114	The lightweight and excellent mechanical properties	1064:1114	The lightweight and excellent mechanical properties promote the application potential of biodegradable composites to replace petroleum-based plastics.					
34537292	2	9	theme	acetylated	267:276	arg1	powder					289:294	the directly acetylated eucalyptus powder	254:294	the directly acetylated eucalyptus powder	254:294	Cellulose acetate (CA) was isolated from the directly acetylated eucalyptus powder after hydrothermal treatment to selectively remove hemicellulose without delignification.					
34537292	0	10	theme	polylactic	111:120	arg1	acid					122:125	polylactic acid	111:125	polylactic acid	111:125	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	0	11	from	eucalyptus	50:59	arg1	separation					10:19	Efficient separation	0:19	Efficient separation of acetylated cellulose from eucalyptus	0:59	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	0	11	from	eucalyptus	50:59	arg1	enhancement					69:79	its enhancement	65:79	its enhancement on the mechanical strength of polylactic acid	65:125	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	7	12	theme	lightweight	1068:1078	arg1	properties					1105:1114	The lightweight and excellent mechanical properties	1064:1114	The lightweight and excellent mechanical properties	1064:1114	The lightweight and excellent mechanical properties promote the application potential of biodegradable composites to replace petroleum-based plastics.					
34537292	4	13	theme	characterization	624:639	arg1	results					641:647	The characterization results	620:647	The characterization results	620:647	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	4	14	theme	acetylated	667:676	arg1	product					678:684	the acetylated product	663:684	the acetylated product	663:684	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	4	14	theme	acetylated	667:676	arg1	acetate					700:706	cellulose acetate	690:706	cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure	690:791	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	4	15	with	acetate	700:706	arg1	weight					731:736	uniform molecular weight	713:736	uniform molecular weight	713:736	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	4	15	with	acetate	700:706	arg1	stability					752:760	good thermal stability	739:760	good thermal stability	739:760	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	4	15	with	acetate	700:706	arg1	structure					783:791	semi-crystalline structure	766:791	semi-crystalline structure	766:791	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	0	16	theme	Efficient	0:8	arg1	separation					10:19	Efficient separation	0:19	Efficient separation of acetylated cellulose from eucalyptus	0:59	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	5	17	theme	solvent	869:875	arg1	casting					877:883	solvent casting	869:883	solvent casting	869:883	Moreover, CA was used to reinforce polylactic acid (PLA) films prepared by solvent casting.					
34537292	3	18	theme	hydrothermal	502:513	arg1	treatment					515:523	hydrothermal treatment	502:523	hydrothermal treatment	502:523	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	3	19	theme	lignin	555:560	arg1	re-condensation					536:550	the re-condensation	532:550	the re-condensation of lignin during hydrothermal treatment	532:590	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	6	20	theme	composite	897:905	arg1	PLA-CA					890:895	The PLA-CA composite	886:905	The PLA-CA composite with 5 wt% CA	886:919	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	3	21	theme	cellulose	409:417	arg1	yield					436:440	the yield	432:440	the yield of the acetylated product (61.34%)	432:475	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	3	21	theme	cellulose	409:417	arg1	%					425:425	90.75%	420:425	90.75%	420:425	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	3	21	theme	cellulose	409:417	arg1	rate					401:404	The conversion rate	386:404	The conversion rate of cellulose (90.75%)	386:426	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	3	21	theme	cellulose	409:417	arg1	%					474:474	61.34%	469:474	61.34%	469:474	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	6	22	from	%	948:948	arg1	strength					961:968	tensile strength	953:968	tensile strength	953:968	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	6	23	from	%	979:979	arg1	strength					961:968	tensile strength	953:968	tensile strength	953:968	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	2	24	theme	hydrothermal	302:313	arg1	treatment					315:323	hydrothermal treatment	302:323	hydrothermal treatment to selectively remove hemicellulose without delignification	302:383	Cellulose acetate (CA) was isolated from the directly acetylated eucalyptus powder after hydrothermal treatment to selectively remove hemicellulose without delignification.					
34537292	3	25	theme	conversion	390:399	arg1	%					425:425	90.75%	420:425	90.75%	420:425	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	3	25	theme	conversion	390:399	arg1	rate					401:404	The conversion rate	386:404	The conversion rate of cellulose (90.75%)	386:426	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	5	26	theme	polylactic	829:838	arg1	PLA					846:848	PLA	846:848	PLA	846:848	Moreover, CA was used to reinforce polylactic acid (PLA) films prepared by solvent casting.					
34537292	5	26	theme	polylactic	829:838	arg1	acid					840:843	polylactic acid	829:843	polylactic acid (PLA) films prepared by solvent casting	829:883	Moreover, CA was used to reinforce polylactic acid (PLA) films prepared by solvent casting.					
34537292	4	27	theme	semi-crystalline	766:781	arg1	structure					783:791	semi-crystalline structure	766:791	semi-crystalline structure	766:791	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	3	28	theme	product	460:466	arg1	yield					436:440	the yield	432:440	the yield of the acetylated product (61.34%)	432:475	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	3	28	theme	product	460:466	arg1	%					425:425	90.75%	420:425	90.75%	420:425	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	3	28	theme	product	460:466	arg1	rate					401:404	The conversion rate	386:404	The conversion rate of cellulose (90.75%)	386:426	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	3	28	theme	product	460:466	arg1	%					474:474	61.34%	469:474	61.34%	469:474	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	4	29	theme	thermal	744:750	arg1	stability					752:760	good thermal stability	739:760	good thermal stability	739:760	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	0	30	theme	acid	122:125	arg1	strength					99:106	the mechanical strength	84:106	the mechanical strength of polylactic acid	84:125	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	5	31	theme	acid	840:843	arg1	films					851:855	polylactic acid (PLA) films	829:855	polylactic acid (PLA) films prepared by solvent casting	829:883	Moreover, CA was used to reinforce polylactic acid (PLA) films prepared by solvent casting.					
34537292	1	32	theme	cellulose	194:202	arg1	acetate					204:210	cellulose acetate	194:210	cellulose acetate	194:210	A simplified and green strategy was provided for the synthesis of cellulose acetate.					
34537292	6	33	theme	%	948:948	arg1	increase					931:938	an increase	928:938	an increase of 80.63% in tensile strength and 59.51% in Young's modulus	928:998	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	6	34	from	strength	961:968	arg1	increase					931:938	an increase	928:938	an increase of 80.63% in tensile strength and 59.51% in Young's modulus	928:998	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	1	35	theme	acetate	204:210	arg1	synthesis					181:189	the synthesis	177:189	the synthesis of cellulose acetate	177:210	A simplified and green strategy was provided for the synthesis of cellulose acetate.					
34537292	0	36	theme	cellulose	35:43	arg1	separation					10:19	Efficient separation	0:19	Efficient separation of acetylated cellulose from eucalyptus	0:59	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	0	36	theme	cellulose	35:43	arg1	enhancement					69:79	its enhancement	65:79	its enhancement on the mechanical strength of polylactic acid	65:125	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	0	37	from	enhancement	69:79	arg1	strength					99:106	the mechanical strength	84:106	the mechanical strength of polylactic acid	84:125	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	0	38	theme	acetylated	24:33	arg1	cellulose					35:43	acetylated cellulose	24:43	acetylated cellulose	24:43	Efficient separation of acetylated cellulose from eucalyptus and its enhancement on the mechanical strength of polylactic acid.					
34537292	3	39	theme	acetylated	449:458	arg1	product					460:466	the acetylated product	445:466	the acetylated product	445:466	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	6	40	from	increase	931:938	arg1	strength					961:968	tensile strength	953:968	tensile strength	953:968	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	6	41	theme	tensile	953:959	arg1	strength					961:968	tensile strength	953:968	tensile strength	953:968	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	3	42	theme	hydrothermal	569:580	arg1	treatment					582:590	hydrothermal treatment	569:590	hydrothermal treatment	569:590	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	7	43	theme	application	1128:1138	arg1	potential					1140:1148	the application potential	1124:1148	the application potential of biodegradable composites to replace petroleum-based plastics	1124:1212	The lightweight and excellent mechanical properties promote the application potential of biodegradable composites to replace petroleum-based plastics.					
34537292	6	44	theme	%	916:916	arg1	CA					918:919	5 wt% CA	912:919	5 wt% CA	912:919	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	2	45	theme	Cellulose	213:221	arg1	CA					232:233	CA	232:233	CA	232:233	Cellulose acetate (CA) was isolated from the directly acetylated eucalyptus powder after hydrothermal treatment to selectively remove hemicellulose without delignification.					
34537292	2	45	theme	Cellulose	213:221	arg1	acetate					223:229	Cellulose acetate	213:229	Cellulose acetate (CA)	213:234	Cellulose acetate (CA) was isolated from the directly acetylated eucalyptus powder after hydrothermal treatment to selectively remove hemicellulose without delignification.					
34537292	4	46	theme	molecular	721:729	arg1	weight					731:736	uniform molecular weight	713:736	uniform molecular weight	713:736	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	7	47	theme	biodegradable	1153:1165	arg1	composites					1167:1176	biodegradable composites	1153:1176	biodegradable composites	1153:1176	The lightweight and excellent mechanical properties promote the application potential of biodegradable composites to replace petroleum-based plastics.					
34537292	4	48	theme	cellulose	690:698	arg1	product					678:684	the acetylated product	663:684	the acetylated product	663:684	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	4	48	theme	cellulose	690:698	arg1	acetate					700:706	cellulose acetate	690:706	cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure	690:791	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	4	49	theme	good	739:742	arg1	stability					752:760	good thermal stability	739:760	good thermal stability	739:760	The characterization results verified that the acetylated product was cellulose acetate with uniform molecular weight, good thermal stability and semi-crystalline structure.					
34537292	2	50	attach	isolated	240:247	arg2	CA					232:233	CA	232:233	CA	232:233	Cellulose acetate (CA) was isolated from the directly acetylated eucalyptus powder after hydrothermal treatment to selectively remove hemicellulose without delignification.					
34537292	2	50	attach	isolated	240:247	arg1	powder					289:294	the directly acetylated eucalyptus powder	254:294	the directly acetylated eucalyptus powder	254:294	Cellulose acetate (CA) was isolated from the directly acetylated eucalyptus powder after hydrothermal treatment to selectively remove hemicellulose without delignification.					
34537292	2	50	attach	isolated	240:247	arg2	acetate					223:229	Cellulose acetate	213:229	Cellulose acetate (CA)	213:234	Cellulose acetate (CA) was isolated from the directly acetylated eucalyptus powder after hydrothermal treatment to selectively remove hemicellulose without delignification.					
34537292	1	51	theme	simplified	130:139	arg1	strategy					151:158	A simplified and green strategy	128:158	A simplified and green strategy	128:158	A simplified and green strategy was provided for the synthesis of cellulose acetate.					
34537292	3	52	theme	adverse	600:606	arg1	difference					608:617	no adverse difference	597:617	no adverse difference	597:617	The conversion rate of cellulose (90.75%) and the yield of the acetylated product (61.34%) were greatly improved by hydrothermal treatment, while the re-condensation of lignin during hydrothermal treatment made no adverse difference.					
34537292	6	53	with	PLA-CA	890:895	arg1	CA					918:919	5 wt% CA	912:919	5 wt% CA	912:919	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34537292	7	54	theme	petroleum-based	1189:1203	arg1	plastics					1205:1212	petroleum-based plastics	1189:1212	petroleum-based plastics	1189:1212	The lightweight and excellent mechanical properties promote the application potential of biodegradable composites to replace petroleum-based plastics.					
34537292	7	55	theme	composites	1167:1176	arg1	potential					1140:1148	the application potential	1124:1148	the application potential of biodegradable composites to replace petroleum-based plastics	1124:1212	The lightweight and excellent mechanical properties promote the application potential of biodegradable composites to replace petroleum-based plastics.					
34537292	1	56	theme	green	145:149	arg1	strategy					151:158	A simplified and green strategy	128:158	A simplified and green strategy	128:158	A simplified and green strategy was provided for the synthesis of cellulose acetate.					
34537292	6	57	theme	Young	984:988	arg1	modulus					992:998	Young's modulus	984:998	Young's modulus	984:998	The PLA-CA composite with 5 wt% CA showed an increase of 80.63% in tensile strength and 59.51% in Young's modulus, and their density decreased from 1.2427 g/cm3 to 1.0028 g/cm3.					
34638061	3	0	theme	optimum	454:460	arg1	conditions					462:471	the optimum conditions	450:471	the optimum conditions	450:471	Under the optimum conditions, the extraction yields of PTP, PBP and total polysaccharides were 9.107 ± 0.391%, 32.506 ± 0.046% and 41.613 ± 0.437%, respectively.					
34638061	1	1	theme	extraction	174:183	arg1	method					151:156	A novel method	143:156	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish	143:250	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	6	2	theme	scavenging	893:902	arg1	radicals					913:920	scavenging multiple radicals	893:920	scavenging multiple radicals	893:920	Moreover, both polysaccharides exhibited stronger antioxidant activities for scavenging multiple radicals and anti-lipid peroxidation.					
34638061	5	3	theme	glucuronic	749:758	arg1	acid					760:763	glucuronic acid	749:763	glucuronic acid	749:763	PTP and PBP were mainly composed of mannose, glucuronic acid, aminogalactose, glucose, galactose and arabinose.					
34638061	1	4	from	extraction	174:183	arg1	Radish					245:250	Purple-heart Radish	232:250	Purple-heart Radish	232:250	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	2	5	theme	time	378:381	arg1	etc.					383:386	time etc.	378:386	time etc.	378:386	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	2	5	theme	time	378:381	arg1	enzymes					323:329	different enzymes	313:329	different enzymes	313:329	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	6	6	theme	multiple	904:911	arg1	radicals					913:920	scavenging multiple radicals	893:920	scavenging multiple radicals	893:920	Moreover, both polysaccharides exhibited stronger antioxidant activities for scavenging multiple radicals and anti-lipid peroxidation.					
34638061	2	7	theme	enzymes	323:329	arg1	effects					302:308	The effects	298:308	The effects of different enzymes, the ATPS composition, extraction temperature, time etc.	298:386	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	0	8	theme	polysaccharides	101:115	arg1	activity					89:96	antioxidant activity	77:96	antioxidant activity	77:96	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	0	8	theme	polysaccharides	101:115	arg1	extraction					33:42	extraction	33:42	extraction	33:42	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	0	8	theme	polysaccharides	101:115	arg1	characterization					56:71	structural characterization	45:71	structural characterization	45:71	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	0	9	from	characterization	56:71	arg1	Radish					135:140	Purple-heart Radish	122:140	Purple-heart Radish	122:140	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	7	10	theme	cell	1140:1143	arg1	enzymolysis					1125:1135	enzymolysis	1125:1135	enzymolysis	1125:1135	Compared to the conventional extraction methods, EAE-MAATPE achieved higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell.					
34638061	7	10	theme	cell	1140:1143	arg1	rupture					1113:1119	rupture	1113:1119	rupture	1113:1119	Compared to the conventional extraction methods, EAE-MAATPE achieved higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell.					
34638061	2	11	theme	different	313:321	arg1	temperature					365:375	extraction temperature	354:375	extraction temperature	354:375	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	2	11	theme	different	313:321	arg1	etc.					383:386	time etc.	378:386	time etc.	378:386	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	2	11	theme	different	313:321	arg1	enzymes					323:329	different enzymes	313:329	different enzymes	313:329	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	2	11	theme	different	313:321	arg1	composition					341:351	the ATPS composition	332:351	the ATPS composition	332:351	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	8	12	theme	simultaneous	1200:1211	arg1	extraction					1213:1222	simultaneous extraction	1200:1222	simultaneous extraction of different polysaccharides from natural products	1200:1273	Thus, EAE-MAATPE provided an efficient alternative to simultaneous extraction of different polysaccharides from natural products.					
34638061	8	13	from	products	1266:1273	arg1	extraction					1213:1222	simultaneous extraction	1200:1222	simultaneous extraction of different polysaccharides from natural products	1200:1273	Thus, EAE-MAATPE provided an efficient alternative to simultaneous extraction of different polysaccharides from natural products.					
34638061	8	13	from	products	1266:1273	arg1	polysaccharides					1237:1251	different polysaccharides	1227:1251	different polysaccharides from natural products	1227:1273	Thus, EAE-MAATPE provided an efficient alternative to simultaneous extraction of different polysaccharides from natural products.					
34638061	3	14	theme	PTP	499:501	arg1	yields					489:494	the extraction yields	474:494	the extraction yields of PTP, PBP and total polysaccharides	474:532	Under the optimum conditions, the extraction yields of PTP, PBP and total polysaccharides were 9.107 ± 0.391%, 32.506 ± 0.046% and 41.613 ± 0.437%, respectively.					
34638061	3	14	theme	PTP	499:501	arg1	%					552:552	9.107 ± 0.391%	539:552	9.107 ± 0.391%	539:552	Under the optimum conditions, the extraction yields of PTP, PBP and total polysaccharides were 9.107 ± 0.391%, 32.506 ± 0.046% and 41.613 ± 0.437%, respectively.					
34638061	2	15	theme	single-factor	409:421	arg1	experiments					423:433	single-factor experiments	409:433	single-factor experiments	409:433	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	1	16	theme	separation	189:198	arg1	method					151:156	A novel method	143:156	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish	143:250	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	0	17	theme	Purple-heart	122:133	arg1	Radish					135:140	Purple-heart Radish	122:140	Purple-heart Radish	122:140	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	8	18	theme	natural	1258:1264	arg1	products					1266:1273	natural products	1258:1273	natural products	1258:1273	Thus, EAE-MAATPE provided an efficient alternative to simultaneous extraction of different polysaccharides from natural products.					
34638061	0	19	from	extraction	33:42	arg1	Radish					135:140	Purple-heart Radish	122:140	Purple-heart Radish	122:140	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	1	20	theme	diverse	203:209	arg1	polysaccharides					211:225	diverse polysaccharides	203:225	diverse polysaccharides from Purple-heart Radish	203:250	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	2	21	theme	ATPS	336:339	arg1	enzymes					323:329	different enzymes	313:329	different enzymes	313:329	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	2	21	theme	ATPS	336:339	arg1	composition					341:351	the ATPS composition	332:351	the ATPS composition	332:351	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	1	22	theme	polysaccharides	211:225	arg1	separation					189:198	separation	189:198	separation of diverse polysaccharides from Purple-heart Radish	189:250	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	1	22	theme	polysaccharides	211:225	arg1	extraction					174:183	simultaneous extraction	161:183	simultaneous extraction	161:183	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	8	23	theme	polysaccharides	1237:1251	arg1	extraction					1213:1222	simultaneous extraction	1200:1222	simultaneous extraction of different polysaccharides from natural products	1200:1273	Thus, EAE-MAATPE provided an efficient alternative to simultaneous extraction of different polysaccharides from natural products.					
34638061	7	24	theme	conventional	967:978	arg1	methods					991:997	the conventional extraction methods	963:997	the conventional extraction methods	963:997	Compared to the conventional extraction methods, EAE-MAATPE achieved higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell.					
34638061	7	25	theme	extraction	980:989	arg1	methods					991:997	the conventional extraction methods	963:997	the conventional extraction methods	963:997	Compared to the conventional extraction methods, EAE-MAATPE achieved higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell.					
34638061	8	26	theme	different	1227:1235	arg1	polysaccharides					1237:1251	different polysaccharides	1227:1251	different polysaccharides from natural products	1227:1273	Thus, EAE-MAATPE provided an efficient alternative to simultaneous extraction of different polysaccharides from natural products.					
34638061	6	27	theme	stronger	857:864	arg1	activities					878:887	stronger antioxidant activities	857:887	stronger antioxidant activities	857:887	Moreover, both polysaccharides exhibited stronger antioxidant activities for scavenging multiple radicals and anti-lipid peroxidation.					
34638061	1	28	theme	Purple-heart	232:243	arg1	Radish					245:250	Purple-heart Radish	232:250	Purple-heart Radish	232:250	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	3	29	theme	polysaccharides	518:532	arg1	yields					489:494	the extraction yields	474:494	the extraction yields of PTP, PBP and total polysaccharides	474:532	Under the optimum conditions, the extraction yields of PTP, PBP and total polysaccharides were 9.107 ± 0.391%, 32.506 ± 0.046% and 41.613 ± 0.437%, respectively.					
34638061	3	29	theme	polysaccharides	518:532	arg1	%					552:552	9.107 ± 0.391%	539:552	9.107 ± 0.391%	539:552	Under the optimum conditions, the extraction yields of PTP, PBP and total polysaccharides were 9.107 ± 0.391%, 32.506 ± 0.046% and 41.613 ± 0.437%, respectively.					
34638061	0	30	from	activity	89:96	arg1	Radish					135:140	Purple-heart Radish	122:140	Purple-heart Radish	122:140	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	4	31	theme	PBP	658:660	arg1	Mw					638:639	Mw	638:639	Mw of PTP	638:646	By means of HPGPC and PMP-HPLC, Mw of PTP and Mw of PBP were 15935 Da and 27962 Da, respectively.					
34638061	4	31	theme	PBP	658:660	arg1	Mw					652:653	Mw	652:653	Mw of PBP	652:660	By means of HPGPC and PMP-HPLC, Mw of PTP and Mw of PBP were 15935 Da and 27962 Da, respectively.					
34638061	3	32	theme	extraction	478:487	arg1	yields					489:494	the extraction yields	474:494	the extraction yields of PTP, PBP and total polysaccharides	474:532	Under the optimum conditions, the extraction yields of PTP, PBP and total polysaccharides were 9.107 ± 0.391%, 32.506 ± 0.046% and 41.613 ± 0.437%, respectively.					
34638061	3	32	theme	extraction	478:487	arg1	%					552:552	9.107 ± 0.391%	539:552	9.107 ± 0.391%	539:552	Under the optimum conditions, the extraction yields of PTP, PBP and total polysaccharides were 9.107 ± 0.391%, 32.506 ± 0.046% and 41.613 ± 0.437%, respectively.					
34638061	1	33	from	Radish	245:250	arg1	separation					189:198	separation	189:198	separation of diverse polysaccharides from Purple-heart Radish	189:250	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	1	33	from	Radish	245:250	arg1	polysaccharides					211:225	diverse polysaccharides	203:225	diverse polysaccharides from Purple-heart Radish	203:250	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	1	33	from	Radish	245:250	arg1	extraction					174:183	simultaneous extraction	161:183	simultaneous extraction	161:183	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	4	34	theme	PTP	644:646	arg1	Mw					638:639	Mw	638:639	Mw of PTP	638:646	By means of HPGPC and PMP-HPLC, Mw of PTP and Mw of PBP were 15935 Da and 27962 Da, respectively.					
34638061	4	34	theme	PTP	644:646	arg1	Mw					652:653	Mw	652:653	Mw of PBP	652:660	By means of HPGPC and PMP-HPLC, Mw of PTP and Mw of PBP were 15935 Da and 27962 Da, respectively.					
34638061	0	35	theme	structural	45:54	arg1	characterization					56:71	structural characterization	45:71	structural characterization	45:71	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	0	36	from	Radish	135:140	arg1	activity					89:96	antioxidant activity	77:96	antioxidant activity	77:96	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	0	36	from	Radish	135:140	arg1	polysaccharides					101:115	polysaccharides	101:115	polysaccharides from Purple-heart Radish	101:140	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	0	36	from	Radish	135:140	arg1	extraction					33:42	extraction	33:42	extraction	33:42	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	0	36	from	Radish	135:140	arg1	characterization					56:71	structural characterization	45:71	structural characterization	45:71	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	7	37	theme	extraction	1027:1036	arg1	efficiency					1038:1047	higher extraction efficiency	1020:1047	higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell	1020:1143	Compared to the conventional extraction methods, EAE-MAATPE achieved higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell.					
34638061	3	38	theme	total	512:516	arg1	polysaccharides					518:532	total polysaccharides	512:532	total polysaccharides	512:532	Under the optimum conditions, the extraction yields of PTP, PBP and total polysaccharides were 9.107 ± 0.391%, 32.506 ± 0.046% and 41.613 ± 0.437%, respectively.					
34638061	6	39	theme	antioxidant	866:876	arg1	activities					878:887	stronger antioxidant activities	857:887	stronger antioxidant activities	857:887	Moreover, both polysaccharides exhibited stronger antioxidant activities for scavenging multiple radicals and anti-lipid peroxidation.					
34638061	1	40	theme	novel	145:149	arg1	method					151:156	A novel method	143:156	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish	143:250	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	7	41	theme	synergistic	1060:1070	arg1	effect					1072:1077	the synergistic effect	1056:1077	the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell	1056:1143	Compared to the conventional extraction methods, EAE-MAATPE achieved higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell.					
34638061	7	42	theme	due	1049:1051	arg1	efficiency					1038:1047	higher extraction efficiency	1020:1047	higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell	1020:1143	Compared to the conventional extraction methods, EAE-MAATPE achieved higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell.					
34638061	3	43	theme	PBP	504:506	arg1	yields					489:494	the extraction yields	474:494	the extraction yields of PTP, PBP and total polysaccharides	474:532	Under the optimum conditions, the extraction yields of PTP, PBP and total polysaccharides were 9.107 ± 0.391%, 32.506 ± 0.046% and 41.613 ± 0.437%, respectively.					
34638061	3	43	theme	PBP	504:506	arg1	%					552:552	9.107 ± 0.391%	539:552	9.107 ± 0.391%	539:552	Under the optimum conditions, the extraction yields of PTP, PBP and total polysaccharides were 9.107 ± 0.391%, 32.506 ± 0.046% and 41.613 ± 0.437%, respectively.					
34638061	2	44	theme	extraction	354:363	arg1	temperature					365:375	extraction temperature	354:375	extraction temperature	354:375	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	2	44	theme	extraction	354:363	arg1	enzymes					323:329	different enzymes	313:329	different enzymes	313:329	The effects of different enzymes, the ATPS composition, extraction temperature, time etc. were investigated by single-factor experiments and RSM.					
34638061	0	45	theme	antioxidant	77:87	arg1	activity					89:96	antioxidant activity	77:96	antioxidant activity	77:96	Enzyme and microwave co-assisted extraction, structural characterization and antioxidant activity of polysaccharides from Purple-heart Radish.					
34638061	7	46	theme	higher	1020:1025	arg1	efficiency					1038:1047	higher extraction efficiency	1020:1047	higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell	1020:1143	Compared to the conventional extraction methods, EAE-MAATPE achieved higher extraction efficiency due to the synergistic effect between EAE and MAATPE leading to rupture and enzymolysis of cell.					
34638061	8	47	from	extraction	1213:1222	arg1	products					1266:1273	natural products	1258:1273	natural products	1258:1273	Thus, EAE-MAATPE provided an efficient alternative to simultaneous extraction of different polysaccharides from natural products.					
34638061	6	48	theme	anti-lipid	926:935	arg1	peroxidation					937:948	anti-lipid peroxidation	926:948	anti-lipid peroxidation	926:948	Moreover, both polysaccharides exhibited stronger antioxidant activities for scavenging multiple radicals and anti-lipid peroxidation.					
34638061	1	49	from	separation	189:198	arg1	Radish					245:250	Purple-heart Radish	232:250	Purple-heart Radish	232:250	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	1	50	theme	simultaneous	161:172	arg1	extraction					174:183	simultaneous extraction	161:183	simultaneous extraction	161:183	A novel method of simultaneous extraction and separation of diverse polysaccharides from Purple-heart Radish was developed by integrating EAE with MAATPE.					
34638061	8	51	theme	efficient	1175:1183	arg1	alternative					1185:1195	an efficient alternative	1172:1195	an efficient alternative to simultaneous extraction of different polysaccharides from natural products	1172:1273	Thus, EAE-MAATPE provided an efficient alternative to simultaneous extraction of different polysaccharides from natural products.					
32437608	7	0	theme	glucose	1318:1324	arg1	GGR					1343:1345	GGR	1343:1345	GGR	1343:1345	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	7	0	theme	glucose	1318:1324	arg1	rate					1337:1340	glucose generation rate	1318:1340	the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine	1314:1449	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	8	1	theme	digestible	1493:1502	arg1	carbohydrate					1504:1515	a slowly digestible carbohydrate	1484:1515	a slowly digestible carbohydrate in mammals	1484:1526	Raw wheat starch is known to be a slowly digestible carbohydrate in mammals and was used as a benchmark control.					
32437608	8	2	theme	wheat	1456:1460	arg1	starch					1462:1467	Raw wheat starch	1452:1467	Raw wheat starch	1452:1467	Raw wheat starch is known to be a slowly digestible carbohydrate in mammals and was used as a benchmark control.					
32437608	10	3	theme	modified	1753:1760	arg1	IMMP					1762:1765	the branching sucrase modified IMMP	1731:1765	the branching sucrase modified IMMP	1731:1765	Interestingly, the digestibility of the branching sucrase modified IMMP and dextran products considerably decreased with increasing percentages of (α1→3)-linkages present.					
32437608	7	4	theme	in	1247:1248	arg1	digestibility					1256:1268	The in vitro digestibility	1243:1268	The in vitro digestibility of the various α-glucans	1243:1293	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	4	5	theme	donor	619:623	arg1	sucrose					606:612	sucrose	606:612	sucrose	606:612	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	4	5	theme	donor	619:623	arg1	substrate					625:633	a donor substrate	617:633	a donor substrate	617:633	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	10	6	theme	branching	1735:1743	arg1	IMMP					1762:1765	the branching sucrase modified IMMP	1731:1765	the branching sucrase modified IMMP	1731:1765	Interestingly, the digestibility of the branching sucrase modified IMMP and dextran products considerably decreased with increasing percentages of (α1→3)-linkages present.					
32437608	1	7	theme	glycosyl	208:215	arg1	family					232:237	the glycosyl hydrolase (GH) family 70 (GH70)	204:247	the glycosyl hydrolase (GH) family 70 (GH70)	204:247	Previously, we have identified and characterized 4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains.					
32437608	7	8	theme	various	1277:1283	arg1	α-glucans					1285:1293	the various α-glucans	1273:1293	the various α-glucans	1273:1293	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	10	9	theme	products	1779:1786	arg1	digestibility					1714:1726	the digestibility	1710:1726	the digestibility of the branching sucrase modified IMMP and dextran products	1710:1786	Interestingly, the digestibility of the branching sucrase modified IMMP and dextran products considerably decreased with increasing percentages of (α1→3)-linkages present.					
32437608	12	10	theme	starch	2062:2067	arg1	α-glucans					2077:2085	These starch derived α-glucans	2056:2085	These starch derived α-glucans	2056:2085	These starch derived α-glucans may find applications in the food industry.					
32437608	6	11	theme	-linked	1175:1181	arg1	units					1191:1195	single (α1→3)-linked glucose units	1162:1195	single (α1→3)-linked glucose units	1162:1195	The products obtained have been structurally characterized in detail, revealing the addition of single (α1→3)-linked glucose units to IMMP (resulting in a comb-like structure).					
32437608	5	12	theme	sucrase	949:955	arg1	domain					912:917	the catalytic domain 2	898:919	the catalytic domain 2 of the L. kunkeei branching sucrase	898:955	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	5	12	theme	sucrase	949:955	arg1	sucrase					949:955	the L. kunkeei branching sucrase	924:955	the L. kunkeei branching sucrase	924:955	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	3	13	theme	branching	519:527	arg1	enzymes					537:543	branching sucrase enzymes	519:543	branching sucrase enzymes belonging to GH70	519:561	Over the years, also, branching sucrase enzymes belonging to GH70 have been characterized.					
32437608	4	14	dep	Lactobacillus	835:847	arg1	kunkeei					849:855	kunkeei	849:855	kunkeei	849:855	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	7	15	theme	upper	1435:1439	arg1	intestine					1441:1449	the upper intestine	1431:1449	the upper intestine	1431:1449	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	5	16	used	used	957:960	arg2	domain					912:917	the catalytic domain 2	898:919	the catalytic domain 2 of the L. kunkeei branching sucrase	898:955	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	5	16	used	used	957:960	arg2	sucrase					949:955	the L. kunkeei branching sucrase	924:955	the L. kunkeei branching sucrase	924:955	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	6	17	theme	single	1162:1167	arg1	units					1191:1195	single (α1→3)-linked glucose units	1162:1195	single (α1→3)-linked glucose units	1162:1195	The products obtained have been structurally characterized in detail, revealing the addition of single (α1→3)-linked glucose units to IMMP (resulting in a comb-like structure).					
32437608	5	18	theme	L. kunkeei	928:937	arg1	sucrase					949:955	the L. kunkeei branching sucrase	924:955	the L. kunkeei branching sucrase	924:955	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	7	19	theme	digestive	1410:1418	arg1	enzymes					1420:1426	the digestive enzymes	1406:1426	the digestive enzymes in the upper intestine	1406:1449	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	4	20	dep	Leuconostoc	794:804	arg1	citreum					806:812	Leuconostoc citreum	794:812	Leuconostoc citreum	794:812	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	1	21	theme	4,6-α-glucanotransferase	168:191	arg1	enzymes					193:199	4,6-α-glucanotransferase enzymes	168:199	4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains	168:333	Previously, we have identified and characterized 4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains.					
32437608	0	22	theme	Branching	92:100	arg1	Enzymes					110:116	4,6-α-Glucanotransferase and Branching Sucrase Enzymes	63:116	4,6-α-Glucanotransferase and Branching Sucrase Enzymes	63:116	Development of Slowly Digestible Starch Derived α-Glucans with 4,6-α-Glucanotransferase and Branching Sucrase Enzymes.					
32437608	1	23	theme	linear	321:326	arg1	chains					328:333	linear chains	321:333	linear chains	321:333	Previously, we have identified and characterized 4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains.					
32437608	4	24	theme	-α-d-Glcp-	711:720	arg1	-branches					783:791	single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches	696:791	single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes)	696:864	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	10	25	theme	-linkages	1848:1856	arg1	present					1858:1864	(α1→3)-linkages present	1842:1864	(α1→3)-linkages present	1842:1864	Interestingly, the digestibility of the branching sucrase modified IMMP and dextran products considerably decreased with increasing percentages of (α1→3)-linkages present.					
32437608	7	26	theme	intestinal	1368:1377	arg1	powder					1387:1392	rat intestinal acetone powder	1364:1392	rat intestinal acetone powder	1364:1392	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	9	27	theme	wheat	1581:1585	arg1	starch					1587:1592	raw wheat starch	1577:1592	raw wheat starch	1577:1592	Compared to raw wheat starch, IMMP and dextran showed reduced digestibility, with partially digestible and indigestible portions.					
32437608	12	28	theme	food	2116:2119	arg1	industry					2121:2128	the food industry	2112:2128	the food industry	2112:2128	These starch derived α-glucans may find applications in the food industry.					
32437608	6	29	theme	units	1191:1195	arg1	addition					1150:1157	the addition	1146:1157	the addition of single (α1→3)-linked glucose units to IMMP (resulting in a comb-like structure)	1146:1240	The products obtained have been structurally characterized in detail, revealing the addition of single (α1→3)-linked glucose units to IMMP (resulting in a comb-like structure).					
32437608	2	30	with	-linkages	486:494	arg1	%					478:478	90%	476:478	90%	476:478	The 4,6-α-glucanotransferase of Lactobacillus reuteri 121, for instance, converts amylose into an isomalto/malto-polysaccharide (IMMP) with 90% (α1→6)-linkages.					
32437608	2	31	theme	isomalto/malto-polysaccharide	434:462	arg1	-linkages					486:494	an isomalto/malto-polysaccharide (IMMP) with 90% (α1→6)-linkages	431:494	an isomalto/malto-polysaccharide (IMMP) with 90% (α1→6)-linkages	431:494	The 4,6-α-glucanotransferase of Lactobacillus reuteri 121, for instance, converts amylose into an isomalto/malto-polysaccharide (IMMP) with 90% (α1→6)-linkages.					
32437608	2	31	theme	isomalto/malto-polysaccharide	434:462	arg1	α1→6					481:484	α1→6	481:484	α1→6	481:484	The 4,6-α-glucanotransferase of Lactobacillus reuteri 121, for instance, converts amylose into an isomalto/malto-polysaccharide (IMMP) with 90% (α1→6)-linkages.					
32437608	12	32	theme	derived	2069:2075	arg1	α-glucans					2077:2085	These starch derived α-glucans	2056:2085	These starch derived α-glucans	2056:2085	These starch derived α-glucans may find applications in the food industry.					
32437608	11	33	theme	derived	2001:2007	arg1	α-glucans					2009:2017	amylose/starch derived α-glucans	1986:2017	amylose/starch derived α-glucans with markedly reduced digestibility	1986:2053	The treatment of amylose with 4,6-α-glucanotransferase and branching sucrase/sucrose thus allowed for the synthesis of amylose/starch derived α-glucans with markedly reduced digestibility.					
32437608	5	34	theme	-α-d-Glcp-	1040:1049	arg1	-branches					1055:1063	-(1→3,6)-α-d-Glcp-(1→6)-branches	1032:1063	-branches	1055:1063	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	11	35	with	treatment	1871:1879	arg1	4,6-α-glucanotransferase					1897:1920	4,6-α-glucanotransferase	1897:1920	4,6-α-glucanotransferase	1897:1920	The treatment of amylose with 4,6-α-glucanotransferase and branching sucrase/sucrose thus allowed for the synthesis of amylose/starch derived α-glucans with markedly reduced digestibility.					
32437608	11	35	with	treatment	1871:1879	arg1	sucrase/sucrose					1936:1950	branching sucrase/sucrose	1926:1950	branching sucrase/sucrose	1926:1950	The treatment of amylose with 4,6-α-glucanotransferase and branching sucrase/sucrose thus allowed for the synthesis of amylose/starch derived α-glucans with markedly reduced digestibility.					
32437608	0	36	theme	Starch	33:38	arg1	Development					0:10	Development	0:10	Development of Slowly Digestible Starch	0:38	Development of Slowly Digestible Starch Derived α-Glucans with 4,6-α-Glucanotransferase and Branching Sucrase Enzymes.					
32437608	5	37	theme	catalytic	902:910	arg1	domain					912:917	the catalytic domain 2	898:919	the catalytic domain 2 of the L. kunkeei branching sucrase	898:955	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	5	37	theme	catalytic	902:910	arg1	sucrase					949:955	the L. kunkeei branching sucrase	924:955	the L. kunkeei branching sucrase	924:955	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	11	38	theme	reduced	2033:2039	arg1	digestibility					2041:2053	markedly reduced digestibility	2024:2053	markedly reduced digestibility	2024:2053	The treatment of amylose with 4,6-α-glucanotransferase and branching sucrase/sucrose thus allowed for the synthesis of amylose/starch derived α-glucans with markedly reduced digestibility.					
32437608	0	39	theme	4,6-α-Glucanotransferase	63:86	arg1	Enzymes					110:116	4,6-α-Glucanotransferase and Branching Sucrase Enzymes	63:116	4,6-α-Glucanotransferase and Branching Sucrase Enzymes	63:116	Development of Slowly Digestible Starch Derived α-Glucans with 4,6-α-Glucanotransferase and Branching Sucrase Enzymes.					
32437608	1	40	theme	α1→6	303:306	arg1	-linkages					308:316	(α1→6)-linkages	302:316	(α1→6)-linkages in linear chains	302:333	Previously, we have identified and characterized 4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains.					
32437608	7	41	theme	generation	1326:1335	arg1	GGR					1343:1345	GGR	1343:1345	GGR	1343:1345	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	7	41	theme	generation	1326:1335	arg1	rate					1337:1340	glucose generation rate	1318:1340	the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine	1314:1449	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	9	42	theme	digestible	1657:1666	arg1	portions					1685:1692	partially digestible and indigestible portions	1647:1692	partially digestible and indigestible portions	1647:1692	Compared to raw wheat starch, IMMP and dextran showed reduced digestibility, with partially digestible and indigestible portions.					
32437608	4	43	theme	Leuconostoc	729:739	arg1	enzyme					749:754	Leuconostoc citreum enzyme	729:754	Leuconostoc citreum enzyme	729:754	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	4	43	theme	Leuconostoc	729:739	arg1	-α-d-Glcp-					711:720	(1→2,6)-α-d-Glcp-(1→6)-	704:726	(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme)	704:755	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	0	44	with	α-Glucans	48:56	arg1	Enzymes					110:116	4,6-α-Glucanotransferase and Branching Sucrase Enzymes	63:116	4,6-α-Glucanotransferase and Branching Sucrase Enzymes	63:116	Development of Slowly Digestible Starch Derived α-Glucans with 4,6-α-Glucanotransferase and Branching Sucrase Enzymes.					
32437608	9	45	theme	indigestible	1672:1683	arg1	portions					1685:1692	partially digestible and indigestible portions	1647:1692	partially digestible and indigestible portions	1647:1692	Compared to raw wheat starch, IMMP and dextran showed reduced digestibility, with partially digestible and indigestible portions.					
32437608	4	46	theme	-α-d-Glcp-	768:777	arg1	-branches					783:791	single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches	696:791	single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes)	696:864	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	1	47	theme	α1→4	262:265	arg1	-linkages					267:275	(α1→4)-linkages	261:275	(α1→4)-linkages in amylose	261:286	Previously, we have identified and characterized 4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains.					
32437608	7	48	theme	α-glucans	1285:1293	arg1	digestibility					1256:1268	The in vitro digestibility	1243:1268	The in vitro digestibility of the various α-glucans	1243:1293	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	8	49	theme	Raw	1452:1454	arg1	starch					1462:1467	Raw wheat starch	1452:1467	Raw wheat starch	1452:1467	Raw wheat starch is known to be a slowly digestible carbohydrate in mammals and was used as a benchmark control.					
32437608	10	50	theme	sucrase	1745:1751	arg1	IMMP					1762:1765	the branching sucrase modified IMMP	1731:1765	the branching sucrase modified IMMP	1731:1765	Interestingly, the digestibility of the branching sucrase modified IMMP and dextran products considerably decreased with increasing percentages of (α1→3)-linkages present.					
32437608	4	51	dep	-branches	783:791	arg1	Leuconostoc					794:804	Leuconostoc	794:804	Leuconostoc	794:804	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	4	51	dep	-branches	783:791	arg1	enzymes					857:863	Lactobacillus kunkeei enzymes	835:863	Lactobacillus kunkeei enzymes	835:863	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	4	51	dep	-branches	783:791	arg1	fallax					827:832	fallax	827:832	fallax	827:832	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	1	52	from	-linkages	267:275	arg1	amylose					280:286	amylose	280:286	amylose	280:286	Previously, we have identified and characterized 4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains.					
32437608	3	53	theme	sucrase	529:535	arg1	enzymes					537:543	branching sucrase enzymes	519:543	branching sucrase enzymes belonging to GH70	519:561	Over the years, also, branching sucrase enzymes belonging to GH70 have been characterized.					
32437608	1	54	theme	hydrolase	217:225	arg1	family					232:237	the glycosyl hydrolase (GH) family 70 (GH70)	204:247	the glycosyl hydrolase (GH) family 70 (GH70)	204:247	Previously, we have identified and characterized 4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains.					
32437608	5	55	theme	acceptor	1000:1007	arg1	dextran					971:977	dextran	971:977	not only dextran but also IMMP	962:991	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	5	55	theme	acceptor	1000:1007	arg1	IMMP					988:991	IMMP	988:991	not only dextran but also IMMP	962:991	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	5	55	theme	acceptor	1000:1007	arg1	substrate					1009:1017	the acceptor substrate	996:1017	the acceptor substrate	996:1017	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	11	56	with	α-glucans	2009:2017	arg1	digestibility					2041:2053	markedly reduced digestibility	2024:2053	markedly reduced digestibility	2024:2053	The treatment of amylose with 4,6-α-glucanotransferase and branching sucrase/sucrose thus allowed for the synthesis of amylose/starch derived α-glucans with markedly reduced digestibility.					
32437608	10	57	theme	dextran	1771:1777	arg1	products					1779:1786	dextran products	1771:1786	dextran products	1771:1786	Interestingly, the digestibility of the branching sucrase modified IMMP and dextran products considerably decreased with increasing percentages of (α1→3)-linkages present.					
32437608	6	58	theme	glucose	1183:1189	arg1	units					1191:1195	single (α1→3)-linked glucose units	1162:1195	single (α1→3)-linked glucose units	1162:1195	The products obtained have been structurally characterized in detail, revealing the addition of single (α1→3)-linked glucose units to IMMP (resulting in a comb-like structure).					
32437608	1	59	theme	GH	228:229	arg1	family					232:237	the glycosyl hydrolase (GH) family 70 (GH70)	204:247	the glycosyl hydrolase (GH) family 70 (GH70)	204:247	Previously, we have identified and characterized 4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains.					
32437608	11	60	theme	amylose	1884:1890	arg1	treatment					1871:1879	The treatment	1867:1879	The treatment of amylose with 4,6-α-glucanotransferase and branching sucrase/sucrose	1867:1950	The treatment of amylose with 4,6-α-glucanotransferase and branching sucrase/sucrose thus allowed for the synthesis of amylose/starch derived α-glucans with markedly reduced digestibility.					
32437608	10	61	theme	IMMP	1762:1765	arg1	digestibility					1714:1726	the digestibility	1710:1726	the digestibility of the branching sucrase modified IMMP and dextran products	1710:1786	Interestingly, the digestibility of the branching sucrase modified IMMP and dextran products considerably decreased with increasing percentages of (α1→3)-linkages present.					
32437608	1	62	theme	family	232:237	arg1	enzymes					193:199	4,6-α-glucanotransferase enzymes	168:199	4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains	168:333	Previously, we have identified and characterized 4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains.					
32437608	8	63	theme	benchmark	1546:1554	arg1	control					1556:1562	a benchmark control	1544:1562	a benchmark control	1544:1562	Raw wheat starch is known to be a slowly digestible carbohydrate in mammals and was used as a benchmark control.					
32437608	7	64	dep	in	1247:1248	arg1	vitro					1250:1254	vitro	1250:1254	vitro	1250:1254	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	11	65	theme	branching	1926:1934	arg1	sucrase/sucrose					1936:1950	branching sucrase/sucrose	1926:1950	branching sucrase/sucrose	1926:1950	The treatment of amylose with 4,6-α-glucanotransferase and branching sucrase/sucrose thus allowed for the synthesis of amylose/starch derived α-glucans with markedly reduced digestibility.					
32437608	4	66	theme	acceptor	664:671	arg1	substrate					673:681	an acceptor substrate	661:681	an acceptor substrate	661:681	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	4	66	theme	acceptor	664:671	arg1	dextran					650:656	dextran	650:656	dextran	650:656	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	11	67	link	derived	2001:2007	arg1	α-glucans					2009:2017	amylose/starch derived α-glucans	1986:2017	amylose/starch derived α-glucans with markedly reduced digestibility	1986:2053	The treatment of amylose with 4,6-α-glucanotransferase and branching sucrase/sucrose thus allowed for the synthesis of amylose/starch derived α-glucans with markedly reduced digestibility.					
32437608	6	68	theme	comb-like	1221:1229	arg1	structure					1231:1239	a comb-like structure	1219:1239	a comb-like structure	1219:1239	The products obtained have been structurally characterized in detail, revealing the addition of single (α1→3)-linked glucose units to IMMP (resulting in a comb-like structure).					
32437608	5	69	theme	branching	939:947	arg1	sucrase					949:955	the L. kunkeei branching sucrase	924:955	the L. kunkeei branching sucrase	924:955	In this work, we observed that the catalytic domain 2 of the L. kunkeei branching sucrase used not only dextran but also IMMP as the acceptor substrate, introducing -(1→3,6)-α-d-Glcp-(1→6)-branches.					
32437608	12	70	link	derived	2069:2075	arg1	α-glucans					2077:2085	These starch derived α-glucans	2056:2085	These starch derived α-glucans	2056:2085	These starch derived α-glucans may find applications in the food industry.					
32437608	9	71	theme	raw	1577:1579	arg1	starch					1587:1592	raw wheat starch	1577:1592	raw wheat starch	1577:1592	Compared to raw wheat starch, IMMP and dextran showed reduced digestibility, with partially digestible and indigestible portions.					
32437608	0	72	theme	Sucrase	102:108	arg1	Enzymes					110:116	4,6-α-Glucanotransferase and Branching Sucrase Enzymes	63:116	4,6-α-Glucanotransferase and Branching Sucrase Enzymes	63:116	Development of Slowly Digestible Starch Derived α-Glucans with 4,6-α-Glucanotransferase and Branching Sucrase Enzymes.					
32437608	7	73	from	enzymes	1420:1426	arg1	intestine					1441:1449	the upper intestine	1431:1449	the upper intestine	1431:1449	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	7	74	theme	acetone	1379:1385	arg1	powder					1387:1392	rat intestinal acetone powder	1364:1392	rat intestinal acetone powder	1364:1392	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	10	75	theme	present	1858:1864	arg1	percentages					1827:1837	percentages	1827:1837	percentages of (α1→3)-linkages present	1827:1864	Interestingly, the digestibility of the branching sucrase modified IMMP and dextran products considerably decreased with increasing percentages of (α1→3)-linkages present.					
32437608	7	76	theme	rat	1364:1366	arg1	powder					1387:1392	rat intestinal acetone powder	1364:1392	rat intestinal acetone powder	1364:1392	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	4	77	theme	Lactobacillus	835:847	arg1	enzymes					857:863	Lactobacillus kunkeei enzymes	835:863	Lactobacillus kunkeei enzymes	835:863	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	4	77	theme	Lactobacillus	835:847	arg1	Leuconostoc					794:804	Leuconostoc	794:804	Leuconostoc	794:804	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	2	78	theme	121	390:392	arg1	4,6-α-glucanotransferase					340:363	The 4,6-α-glucanotransferase	336:363	The 4,6-α-glucanotransferase of Lactobacillus reuteri 121	336:392	The 4,6-α-glucanotransferase of Lactobacillus reuteri 121, for instance, converts amylose into an isomalto/malto-polysaccharide (IMMP) with 90% (α1→6)-linkages.					
32437608	4	79	theme	single	696:701	arg1	-branches					783:791	single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches	696:791	single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes)	696:864	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	2	80	theme	Lactobacillus	368:380	arg1	121					390:392	Lactobacillus reuteri 121	368:392	Lactobacillus reuteri 121	368:392	The 4,6-α-glucanotransferase of Lactobacillus reuteri 121, for instance, converts amylose into an isomalto/malto-polysaccharide (IMMP) with 90% (α1→6)-linkages.					
32437608	0	81	theme	Digestible	22:31	arg1	Starch					33:38	Slowly Digestible Starch	15:38	Slowly Digestible Starch	15:38	Development of Slowly Digestible Starch Derived α-Glucans with 4,6-α-Glucanotransferase and Branching Sucrase Enzymes.					
32437608	11	82	theme	α-glucans	2009:2017	arg1	synthesis					1973:1981	the synthesis	1969:1981	the synthesis of amylose/starch derived α-glucans with markedly reduced digestibility	1969:2053	The treatment of amylose with 4,6-α-glucanotransferase and branching sucrase/sucrose thus allowed for the synthesis of amylose/starch derived α-glucans with markedly reduced digestibility.					
32437608	9	83	theme	reduced	1619:1625	arg1	digestibility					1627:1639	reduced digestibility	1619:1639	reduced digestibility	1619:1639	Compared to raw wheat starch, IMMP and dextran showed reduced digestibility, with partially digestible and indigestible portions.					
32437608	4	84	theme	citreum	741:747	arg1	enzyme					749:754	Leuconostoc citreum enzyme	729:754	Leuconostoc citreum enzyme	729:754	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	4	84	theme	citreum	741:747	arg1	-α-d-Glcp-					711:720	(1→2,6)-α-d-Glcp-(1→6)-	704:726	(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme)	704:755	These enzymes use sucrose as a donor substrate to glucosylate dextran as an acceptor substrate, introducing single -(1→2,6)-α-d-Glcp-(1→6)- (Leuconostoc citreum enzyme) or -(1→3,6)-α-d-Glcp-(1→6)-branches (Leuconostoc citreum, Leuconostoc fallax, Lactobacillus kunkeei enzymes).					
32437608	1	85	from	-linkages	308:316	arg1	chains					328:333	linear chains	321:333	linear chains	321:333	Previously, we have identified and characterized 4,6-α-glucanotransferase enzymes of the glycosyl hydrolase (GH) family 70 (GH70) that cleave (α1→4)-linkages in amylose and introduce (α1→6)-linkages in linear chains.					
32437608	8	86	from	carbohydrate	1504:1515	arg1	mammals					1520:1526	mammals	1520:1526	mammals	1520:1526	Raw wheat starch is known to be a slowly digestible carbohydrate in mammals and was used as a benchmark control.					
32437608	2	87	dep	Lactobacillus	368:380	arg1	reuteri					382:388	reuteri	382:388	reuteri	382:388	The 4,6-α-glucanotransferase of Lactobacillus reuteri 121, for instance, converts amylose into an isomalto/malto-polysaccharide (IMMP) with 90% (α1→6)-linkages.					
32437608	7	88	theme	rate	1337:1340	arg1	assay					1348:1352	the glucose generation rate (GGR) assay	1314:1352	the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine	1314:1449	The in vitro digestibility of the various α-glucans was estimated with the glucose generation rate (GGR) assay that uses rat intestinal acetone powder to simulate the digestive enzymes in the upper intestine.					
32437608	6	89	link	-linked	1175:1181	arg1	units					1191:1195	single (α1→3)-linked glucose units	1162:1195	single (α1→3)-linked glucose units	1162:1195	The products obtained have been structurally characterized in detail, revealing the addition of single (α1→3)-linked glucose units to IMMP (resulting in a comb-like structure).					
33161080	0	0	theme	Coriolus	88:95	arg1	versicolor					97:106	mushroom Coriolus versicolor	79:106	mushroom Coriolus versicolor	79:106	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	6	1	theme	relative	905:912	arg1	weight					929:934	relative high molecular weight	905:934	relative high molecular weight	905:934	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	2	theme	TNF-α	1034:1038	arg1	level					1056:1060	TNF-α mRNA expression level	1034:1060	TNF-α mRNA expression level	1034:1060	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	0	3	theme	mushroom	79:86	arg1	versicolor					97:106	mushroom Coriolus versicolor	79:106	mushroom Coriolus versicolor	79:106	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	6	4	theme	less	940:943	arg1	branches					945:952	less branches	940:952	less branches	940:952	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	5	5	attach	attached	854:861	arg3	site					870:873	O-6 site	866:873	O-6 site	866:873	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	5	5	attach	attached	854:861	arg2	branches					845:852	branches	845:852	branches attached at O-6 site	845:873	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	2	6	theme	ethanol	357:363	arg1	precipitation					365:377	ethanol precipitation	357:377	ethanol precipitation	357:377	Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Coriolus versicolor by water extraction and ethanol precipitation.					
33161080	0	7	from	Isolation	0:8	arg1	bodies					69:74	fruiting bodies	60:74	fruiting bodies of mushroom Coriolus versicolor	60:106	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	5	8	theme	molecular	668:676	arg1	weight					678:683	molecular weight	668:683	molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa	668:726	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	2	9	theme	fruiting	294:301	arg1	bodies					303:308	the dried fruiting bodies	284:308	the dried fruiting bodies of Coriolus versicolor	284:331	Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Coriolus versicolor by water extraction and ethanol precipitation.					
33161080	6	10	theme	iNOS	1025:1028	arg1	induction					973:981	significant induction	961:981	significant induction of NO production	961:998	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	10	theme	iNOS	1025:1028	arg1	phagocytosis					1067:1078	phagocytosis	1067:1078	phagocytosis on RAW 264.7 cells	1067:1097	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	10	theme	iNOS	1025:1028	arg1	augmentation					1009:1020	obvious augmentation	1001:1020	obvious augmentation of iNOS and TNF-α mRNA expression level	1001:1060	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	0	11	theme	versicolor	97:106	arg1	bodies					69:74	fruiting bodies	60:74	fruiting bodies of mushroom Coriolus versicolor	60:106	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	4	12	from	bioactivities	538:550	arg1	cells					577:581	RAW 264.7 macrophages cells	555:581	RAW 264.7 macrophages cells	555:581	The bioactivities on RAW 264.7 macrophages cells were evaluated, and further structure-bioactivity relationships were concluded.					
33161080	7	13	theme	molecular	1177:1185	arg1	weight					1187:1192	high molecular weight	1172:1192	high molecular weight	1172:1192	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	2	14	theme	dried	288:292	arg1	bodies					303:308	the dried fruiting bodies	284:308	the dried fruiting bodies of Coriolus versicolor	284:331	Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Coriolus versicolor by water extraction and ethanol precipitation.					
33161080	7	15	theme	versicolor	1309:1318	arg1	utilization					1285:1295	the utilization	1281:1295	the utilization of Coriolus versicolor	1281:1318	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	6	16	theme	molecular	919:927	arg1	weight					929:934	relative high molecular weight	905:934	relative high molecular weight	905:934	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	5	17	theme	29.7 kDa	688:695	arg1	weight					678:683	molecular weight	668:683	molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa	668:726	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	1	18	theme	edible	184:189	arg1	mushroom					201:208	an edible medicinal mushroom	181:208	an edible medicinal mushroom in China	181:217	Coriolus versicolor is an edible medicinal mushroom in China.					
33161080	1	18	theme	edible	184:189	arg1	versicolor					167:176	Coriolus versicolor	158:176	Coriolus versicolor	158:176	Coriolus versicolor is an edible medicinal mushroom in China.					
33161080	5	19	theme	-β-/	791:794	arg1	group					822:826	(l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group	784:826	(l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group	784:826	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	6	20	theme	high	914:917	arg1	weight					929:934	relative high molecular weight	905:934	relative high molecular weight	905:934	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	3	21	theme	overall	432:438	arg1	consideration					440:452	overall consideration	432:452	overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data	432:531	Their chemical structures were well elucidated with overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data.					
33161080	6	22	from	phagocytosis	1067:1078	arg1	cells					1093:1097	RAW 264.7 cells	1083:1097	RAW 264.7 cells	1083:1097	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	23	theme	level	1056:1060	arg1	induction					973:981	significant induction	961:981	significant induction of NO production	961:998	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	23	theme	level	1056:1060	arg1	phagocytosis					1067:1078	phagocytosis	1067:1078	phagocytosis on RAW 264.7 cells	1067:1097	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	23	theme	level	1056:1060	arg1	augmentation					1009:1020	obvious augmentation	1001:1020	obvious augmentation of iNOS and TNF-α mRNA expression level	1001:1060	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	1	24	theme	medicinal	191:199	arg1	mushroom					201:208	an edible medicinal mushroom	181:208	an edible medicinal mushroom in China	181:217	Coriolus versicolor is an edible medicinal mushroom in China.					
33161080	1	24	theme	medicinal	191:199	arg1	versicolor					167:176	Coriolus versicolor	158:176	Coriolus versicolor	158:176	Coriolus versicolor is an edible medicinal mushroom in China.					
33161080	7	25	theme	immunomodulatory	1213:1228	arg1	ability					1230:1236	enhanced immunomodulatory ability	1204:1236	enhanced immunomodulatory ability	1204:1236	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	5	26	theme	-β-d-glucopyranosyl	802:820	arg1	group					822:826	(l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group	784:826	(l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group	784:826	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	5	27	dep	group	822:826	arg1	l → 4					785:789	l → 4	785:789	l → 4	785:789	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	7	28	contain	possessed	1194:1202	arg2	ability					1230:1236	enhanced immunomodulatory ability	1204:1236	enhanced immunomodulatory ability	1204:1236	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	7	28	contain	possessed	1194:1202	arg1	polysaccharides					1133:1147	CVP polysaccharides	1129:1147	CVP polysaccharides with less branches and high molecular weight	1129:1192	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	2	29	theme	versicolor	322:331	arg1	bodies					303:308	the dried fruiting bodies	284:308	the dried fruiting bodies of Coriolus versicolor	284:331	Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Coriolus versicolor by water extraction and ethanol precipitation.					
33161080	2	30	theme	Coriolus	313:320	arg1	versicolor					322:331	Coriolus versicolor	313:331	Coriolus versicolor	313:331	Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Coriolus versicolor by water extraction and ethanol precipitation.					
33161080	3	31	theme	monosaccharide	457:470	arg1	composition					472:482	monosaccharide composition	457:482	monosaccharide composition	457:482	Their chemical structures were well elucidated with overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data.					
33161080	3	32	theme	chemical	386:393	arg1	structures					395:404	Their chemical structures	380:404	Their chemical structures	380:404	Their chemical structures were well elucidated with overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data.					
33161080	6	33	theme	obvious	1001:1007	arg1	augmentation					1009:1020	obvious augmentation	1001:1020	obvious augmentation of iNOS and TNF-α mRNA expression level	1001:1060	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	0	34	theme	immunomodulatory	132:147	arg1	effects					149:155	their immunomodulatory effects	126:155	their immunomodulatory effects	126:155	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	6	35	theme	RAW	1083:1085	arg1	cells					1093:1097	RAW 264.7 cells	1083:1097	RAW 264.7 cells	1083:1097	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	0	36	theme	structure	14:22	arg1	elucidation					24:34	structure elucidation	14:34	structure elucidation	14:34	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	4	37	theme	structure-bioactivity	611:631	arg1	relationships					633:645	further structure-bioactivity relationships	603:645	further structure-bioactivity relationships	603:645	The bioactivities on RAW 264.7 macrophages cells were evaluated, and further structure-bioactivity relationships were concluded.					
33161080	3	38	theme	1D/2D-NMR	510:518	arg1	spectra					520:526	1D/2D-NMR spectra	510:526	1D/2D-NMR spectra	510:526	Their chemical structures were well elucidated with overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data.					
33161080	0	39	theme	polysaccharides	39:53	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	0	39	theme	polysaccharides	39:53	arg1	elucidation					24:34	structure elucidation	14:34	structure elucidation	14:34	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	0	39	theme	polysaccharides	39:53	arg1	evaluation					112:121	evaluation	112:121	evaluation	112:121	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	4	40	theme	further	603:609	arg1	relationships					633:645	further structure-bioactivity relationships	603:645	further structure-bioactivity relationships	603:645	The bioactivities on RAW 264.7 macrophages cells were evaluated, and further structure-bioactivity relationships were concluded.					
33161080	6	41	from	augmentation	1009:1020	arg1	cells					1093:1097	RAW 264.7 cells	1083:1097	RAW 264.7 cells	1083:1097	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	42	theme	production	989:998	arg1	induction					973:981	significant induction	961:981	significant induction of NO production	961:998	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	42	theme	production	989:998	arg1	phagocytosis					1067:1078	phagocytosis	1067:1078	phagocytosis on RAW 264.7 cells	1067:1097	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	42	theme	production	989:998	arg1	augmentation					1009:1020	obvious augmentation	1001:1020	obvious augmentation of iNOS and TNF-α mRNA expression level	1001:1060	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	43	with	CVPa	895:898	arg1	weight					929:934	relative high molecular weight	905:934	relative high molecular weight	905:934	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	6	43	with	CVPa	895:898	arg1	branches					945:952	less branches	940:952	less branches	940:952	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	3	44	theme	methylation	485:495	arg1	analysis					497:504	methylation analysis	485:504	methylation analysis	485:504	Their chemical structures were well elucidated with overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data.					
33161080	3	45	theme	composition	472:482	arg1	data					528:531	monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data	457:531	monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data	457:531	Their chemical structures were well elucidated with overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data.					
33161080	6	46	theme	NO	986:987	arg1	production					989:998	NO production	986:998	NO production	986:998	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	0	47	theme	effects	149:155	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	0	47	theme	effects	149:155	arg1	elucidation					24:34	structure elucidation	14:34	structure elucidation	14:34	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	0	47	theme	effects	149:155	arg1	evaluation					112:121	evaluation	112:121	evaluation	112:121	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	3	48	theme	analysis	497:504	arg1	data					528:531	monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data	457:531	monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data	457:531	Their chemical structures were well elucidated with overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data.					
33161080	0	49	theme	fruiting	60:67	arg1	bodies					69:74	fruiting bodies	60:74	fruiting bodies of mushroom Coriolus versicolor	60:106	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	4	50	theme	macrophages	565:575	arg1	cells					577:581	RAW 264.7 macrophages cells	555:581	RAW 264.7 macrophages cells	555:581	The bioactivities on RAW 264.7 macrophages cells were evaluated, and further structure-bioactivity relationships were concluded.					
33161080	6	51	from	induction	973:981	arg1	cells					1093:1097	RAW 264.7 cells	1083:1097	RAW 264.7 cells	1083:1097	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	0	52	from	bodies	69:74	arg1	polysaccharides					39:53	polysaccharides	39:53	polysaccharides from fruiting bodies of mushroom Coriolus versicolor	39:106	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	0	52	from	bodies	69:74	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	0	52	from	bodies	69:74	arg1	elucidation					24:34	structure elucidation	14:34	structure elucidation	14:34	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	0	52	from	bodies	69:74	arg1	evaluation					112:121	evaluation	112:121	evaluation	112:121	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	7	53	with	polysaccharides	1133:1147	arg1	weight					1187:1192	high molecular weight	1172:1192	high molecular weight	1172:1192	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	7	53	with	polysaccharides	1133:1147	arg1	branches					1159:1166	branches	1159:1166	branches	1159:1166	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	4	54	theme	RAW	555:557	arg1	macrophages					565:575	RAW 264.7 macrophages	555:575	RAW 264.7 macrophages cells	555:581	The bioactivities on RAW 264.7 macrophages cells were evaluated, and further structure-bioactivity relationships were concluded.					
33161080	7	55	theme	CVP	1129:1131	arg1	polysaccharides					1133:1147	CVP polysaccharides	1129:1147	CVP polysaccharides with less branches and high molecular weight	1129:1192	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	6	56	theme	significant	961:971	arg1	induction					973:981	significant induction	961:981	significant induction of NO production	961:998	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	5	57	theme	isolated	737:744	arg1	backbone					831:838	backbone	831:838	backbone with branches attached at O-6 site	831:873	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	5	57	theme	isolated	737:744	arg1	polysaccharides					746:760	the two isolated polysaccharides	729:760	the two isolated polysaccharides	729:760	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	0	58	from	evaluation	112:121	arg1	bodies					69:74	fruiting bodies	60:74	fruiting bodies of mushroom Coriolus versicolor	60:106	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	7	59	theme	high	1172:1175	arg1	weight					1187:1192	high molecular weight	1172:1192	high molecular weight	1172:1192	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	2	60	theme	water	336:340	arg1	extraction					342:351	water extraction	336:351	water extraction	336:351	Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Coriolus versicolor by water extraction and ethanol precipitation.					
33161080	5	61	with	backbone	831:838	arg1	branches					845:852	branches	845:852	branches attached at O-6 site	845:873	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	1	62	from	mushroom	201:208	arg1	China					213:217	China	213:217	China	213:217	Coriolus versicolor is an edible medicinal mushroom in China.					
33161080	3	63	theme	data	528:531	arg1	consideration					440:452	overall consideration	432:452	overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data	432:531	Their chemical structures were well elucidated with overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data.					
33161080	3	64	theme	spectra	520:526	arg1	data					528:531	monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data	457:531	monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data	457:531	Their chemical structures were well elucidated with overall consideration of monosaccharide composition, methylation analysis and 1D/2D-NMR spectra data.					
33161080	1	65	theme	Coriolus	158:165	arg1	mushroom					201:208	an edible medicinal mushroom	181:208	an edible medicinal mushroom in China	181:217	Coriolus versicolor is an edible medicinal mushroom in China.					
33161080	1	65	theme	Coriolus	158:165	arg1	versicolor					167:176	Coriolus versicolor	158:176	Coriolus versicolor	158:176	Coriolus versicolor is an edible medicinal mushroom in China.					
33161080	6	66	theme	expression	1045:1054	arg1	level					1056:1060	TNF-α mRNA expression level	1034:1060	TNF-α mRNA expression level	1034:1060	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
33161080	0	67	from	elucidation	24:34	arg1	bodies					69:74	fruiting bodies	60:74	fruiting bodies of mushroom Coriolus versicolor	60:106	Isolation and structure elucidation of polysaccharides from fruiting bodies of mushroom Coriolus versicolor and evaluation of their immunomodulatory effects.					
33161080	5	68	theme	O-6	866:868	arg1	site					870:873	O-6 site	866:873	O-6 site	866:873	With molecular weight of 29.7 kDa for CVPn and 50.8 kDa for CVPa, the two isolated polysaccharides were both composed of (l → 4)-β-/(1 → 3)-β-d-glucopyranosyl group as backbone with branches attached at O-6 site.					
33161080	7	69	theme	Coriolus	1300:1307	arg1	versicolor					1309:1318	Coriolus versicolor	1300:1318	Coriolus versicolor	1300:1318	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	7	70	theme	enhanced	1204:1211	arg1	ability					1230:1236	enhanced immunomodulatory ability	1204:1236	enhanced immunomodulatory ability	1204:1236	These results clarified that CVP polysaccharides with less branches and high molecular weight possessed enhanced immunomodulatory ability, and this finding could be a reference for the utilization of Coriolus versicolor.					
33161080	6	71	theme	mRNA	1040:1043	arg1	level					1056:1060	TNF-α mRNA expression level	1034:1060	TNF-α mRNA expression level	1034:1060	Comparing to CVPn, CVPa with relative high molecular weight and less branches showed significant induction of NO production, obvious augmentation of iNOS and TNF-α mRNA expression level, and phagocytosis on RAW 264.7 cells.					
34571125	8	0	theme	alkyl	1058:1062	arg1	chain					1064:1068	The long alkyl chain	1049:1068	The long alkyl chain in QAS	1049:1075	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	8	1	theme	nanocomposites	1153:1166	arg1	nature					1116:1121	the hydrophobic nature	1100:1121	the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites	1100:1166	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	1	2	theme	montmorillonite	191:205	arg1	clay					213:216	montmorillonite (MMT) clay	191:216	montmorillonite (MMT) clay	191:216	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	11	3	theme	Pullulan/agar/MMT	1540:1556	arg1	nanocomposites					1563:1576	the Pullulan/agar/MMT clay nanocomposites	1536:1576	the Pullulan/agar/MMT clay nanocomposites	1536:1576	With the addition of QAS, the Pullulan/agar/MMT clay nanocomposites showed good antimicrobial activity against gram-positive and gram-negative pathogens.					
34571125	8	4	theme	contact	1183:1189	arg1	66.2-71.2°					1198:1207	66.2-71.2°	1198:1207	66.2-71.2°	1198:1207	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	8	4	theme	contact	1183:1189	arg1	angle					1191:1195	the contact angle	1179:1195	the contact angle (66.2-71.2°)	1179:1208	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	9	5	theme	Pullulan/agar/MMT	1317:1333	arg1	clay/QAS					1335:1342	Pullulan/agar/MMT clay/QAS	1317:1342	Pullulan/agar/MMT clay/QAS	1317:1342	The combination of Pullulan/agar/MMT clay/QAS had a synergistic effect on the rheological properties.					
34571125	8	6	theme	long	1053:1056	arg1	chain					1064:1068	The long alkyl chain	1049:1068	The long alkyl chain in QAS	1049:1075	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	6	7	theme	mechanical	854:863	arg1	properties					865:874	The thermal and mechanical properties	838:874	The thermal and mechanical properties	838:874	The thermal and mechanical properties were significantly improved by the MMT clay.					
34571125	2	8	theme	Pullulan/agar/MMT	367:383	arg1	nanocomposites					394:407	The Pullulan/agar/MMT clay/QAS nanocomposites	363:407	The Pullulan/agar/MMT clay/QAS nanocomposites	363:407	The Pullulan/agar/MMT clay/QAS nanocomposites were studied via Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analyses.					
34571125	11	9	theme	QAS	1531:1533	arg1	addition					1519:1526	the addition	1515:1526	the addition of QAS	1515:1533	With the addition of QAS, the Pullulan/agar/MMT clay nanocomposites showed good antimicrobial activity against gram-positive and gram-negative pathogens.					
34571125	1	10	theme	Pullulan/agar/MMT	263:279	arg1	clay/QAS					281:288	Pullulan/agar/MMT clay/QAS	263:288	Pullulan/agar/MMT clay/QAS	263:288	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	1	10	theme	Pullulan/agar/MMT	263:279	arg1	nanocomposites					298:311	silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	243:311	silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	243:311	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	9	11	theme	clay/QAS	1335:1342	arg1	combination					1302:1312	The combination	1298:1312	The combination of Pullulan/agar/MMT clay/QAS	1298:1342	The combination of Pullulan/agar/MMT clay/QAS had a synergistic effect on the rheological properties.					
34571125	10	12	theme	loss	1469:1472	arg1	modulus					1474:1480	the viscosity, storage, and loss modulus	1441:1480	the viscosity, storage, and loss modulus of the hydrogel composites	1441:1507	MMT clay and QAS significantly increased the viscosity, storage, and loss modulus of the hydrogel composites.					
34571125	11	13	theme	clay	1558:1561	arg1	nanocomposites					1563:1576	the Pullulan/agar/MMT clay nanocomposites	1536:1576	the Pullulan/agar/MMT clay nanocomposites	1536:1576	With the addition of QAS, the Pullulan/agar/MMT clay nanocomposites showed good antimicrobial activity against gram-positive and gram-negative pathogens.					
34571125	1	14	dep	pullulan	175:182	arg1	clay/QAS					281:288	Pullulan/agar/MMT clay/QAS	263:288	Pullulan/agar/MMT clay/QAS	263:288	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	1	14	dep	pullulan	175:182	arg1	nanocomposites					298:311	silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	243:311	silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	243:311	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	7	15	from	break	960:964	arg1	elongation					946:955	elongation	946:955	elongation	946:955	The tensile strength and elongation at break of the composites were in the range of 23.8-39.7 MPa and 37.2-26.9%, respectively.					
34571125	7	15	from	break	960:964	arg1	strength					933:940	tensile strength	925:940	tensile strength	925:940	The tensile strength and elongation at break of the composites were in the range of 23.8-39.7 MPa and 37.2-26.9%, respectively.					
34571125	0	16	theme	packaging	123:131	arg1	applications					133:144	packaging applications	123:144	packaging applications	123:144	Montmorillonite clay and quaternary ammonium silane-reinforced pullulan/agar-based nanocomposites and their properties for packaging applications.					
34571125	1	17	dep	simple	332:337	arg1	cost-effective					340:353	cost-effective	340:353	cost-effective	340:353	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	6	18	theme	thermal	842:848	arg1	properties					865:874	The thermal and mechanical properties	838:874	The thermal and mechanical properties	838:874	The thermal and mechanical properties were significantly improved by the MMT clay.					
34571125	2	19	theme	clay/QAS	385:392	arg1	nanocomposites					394:407	The Pullulan/agar/MMT clay/QAS nanocomposites	363:407	The Pullulan/agar/MMT clay/QAS nanocomposites	363:407	The Pullulan/agar/MMT clay/QAS nanocomposites were studied via Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analyses.					
34571125	8	20	theme	vapor	1217:1221	arg1	permeability					1223:1234	water vapor permeability	1211:1234	water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s)	1211:1263	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	8	20	theme	vapor	1217:1221	arg1	3.17-2.20 × 10-9 g/m2 Pa·s					1237:1262	3.17-2.20 × 10-9 g/m2 Pa·s	1237:1262	3.17-2.20 × 10-9 g/m2 Pa·s	1237:1262	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	5	21	theme	clay/QAS	788:795	arg1	transmittance					753:765	The ultraviolet (UV) transmittance	732:765	The ultraviolet (UV) transmittance of Pullulan/agar/MMT clay/QAS	732:795	The ultraviolet (UV) transmittance of Pullulan/agar/MMT clay/QAS was in the range of 91.4-79.8 at 600 nm.					
34571125	2	22	theme	scanning	467:474	arg1	microscopy					485:494	scanning electron microscopy	467:494	scanning electron microscopy	467:494	The Pullulan/agar/MMT clay/QAS nanocomposites were studied via Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analyses.					
34571125	4	23	theme	MMT	722:724	arg1	clay					726:729	MMT clay	722:729	MMT clay	722:729	However, the transparency of the composite was not significantly affected by the addition of MMT clay.					
34571125	1	24	theme	MMT	208:210	arg1	clay					213:216	montmorillonite (MMT) clay	191:216	montmorillonite (MMT) clay	191:216	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	2	25	theme	diffraction	507:517	arg1	analyses					519:526	X-ray diffraction analyses	501:526	X-ray diffraction analyses	501:526	The Pullulan/agar/MMT clay/QAS nanocomposites were studied via Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analyses.					
34571125	9	26	theme	synergistic	1350:1360	arg1	effect					1362:1367	a synergistic effect	1348:1367	a synergistic effect	1348:1367	The combination of Pullulan/agar/MMT clay/QAS had a synergistic effect on the rheological properties.					
34571125	10	27	theme	composites	1498:1507	arg1	modulus					1474:1480	the viscosity, storage, and loss modulus	1441:1480	the viscosity, storage, and loss modulus of the hydrogel composites	1441:1507	MMT clay and QAS significantly increased the viscosity, storage, and loss modulus of the hydrogel composites.					
34571125	7	28	theme	composites	973:982	arg1	elongation					946:955	elongation	946:955	elongation	946:955	The tensile strength and elongation at break of the composites were in the range of 23.8-39.7 MPa and 37.2-26.9%, respectively.					
34571125	7	28	theme	composites	973:982	arg1	strength					933:940	tensile strength	925:940	tensile strength	925:940	The tensile strength and elongation at break of the composites were in the range of 23.8-39.7 MPa and 37.2-26.9%, respectively.					
34571125	8	29	theme	water	1211:1215	arg1	permeability					1223:1234	water vapor permeability	1211:1234	water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s)	1211:1263	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	8	29	theme	water	1211:1215	arg1	3.17-2.20 × 10-9 g/m2 Pa·s					1237:1262	3.17-2.20 × 10-9 g/m2 Pa·s	1237:1262	3.17-2.20 × 10-9 g/m2 Pa·s	1237:1262	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	2	30	theme	Fourier-transform	426:442	arg1	spectroscopy					453:464	Fourier-transform infrared spectroscopy	426:464	Fourier-transform infrared spectroscopy	426:464	The Pullulan/agar/MMT clay/QAS nanocomposites were studied via Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analyses.					
34571125	2	31	theme	X-ray	501:505	arg1	analyses					519:526	X-ray diffraction analyses	501:526	X-ray diffraction analyses	501:526	The Pullulan/agar/MMT clay/QAS nanocomposites were studied via Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analyses.					
34571125	10	32	theme	hydrogel	1489:1496	arg1	composites					1498:1507	the hydrogel composites	1485:1507	the hydrogel composites	1485:1507	MMT clay and QAS significantly increased the viscosity, storage, and loss modulus of the hydrogel composites.					
34571125	10	33	theme	storage	1456:1462	arg1	modulus					1474:1480	the viscosity, storage, and loss modulus	1441:1480	the viscosity, storage, and loss modulus of the hydrogel composites	1441:1507	MMT clay and QAS significantly increased the viscosity, storage, and loss modulus of the hydrogel composites.					
34571125	3	34	theme	nanocomposites	613:626	arg1	properties					595:604	the properties	591:604	the properties of the nanocomposites	591:626	The concentration of MMT clay played a very important role in the properties of the nanocomposites.					
34571125	11	35	theme	gram-negative	1639:1651	arg1	pathogens					1653:1661	gram-positive and gram-negative pathogens	1621:1661	gram-positive and gram-negative pathogens	1621:1661	With the addition of QAS, the Pullulan/agar/MMT clay nanocomposites showed good antimicrobial activity against gram-positive and gram-negative pathogens.					
34571125	1	36	theme	clay	213:216	arg1	combinations					159:170	Synergistic combinations	147:170	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	147:311	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	0	37	theme	Montmorillonite	0:14	arg1	clay					16:19	Montmorillonite clay	0:19	Montmorillonite clay	0:19	Montmorillonite clay and quaternary ammonium silane-reinforced pullulan/agar-based nanocomposites and their properties for packaging applications.					
34571125	1	38	theme	active	291:296	arg1	clay/QAS					281:288	Pullulan/agar/MMT clay/QAS	263:288	Pullulan/agar/MMT clay/QAS	263:288	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	1	38	theme	active	291:296	arg1	nanocomposites					298:311	silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	243:311	silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	243:311	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	11	39	theme	antimicrobial	1590:1602	arg1	activity					1604:1611	good antimicrobial activity	1585:1611	good antimicrobial activity against gram-positive and gram-negative pathogens	1585:1661	With the addition of QAS, the Pullulan/agar/MMT clay nanocomposites showed good antimicrobial activity against gram-positive and gram-negative pathogens.					
34571125	11	40	theme	gram-positive	1621:1633	arg1	pathogens					1653:1661	gram-positive and gram-negative pathogens	1621:1661	gram-positive and gram-negative pathogens	1621:1661	With the addition of QAS, the Pullulan/agar/MMT clay nanocomposites showed good antimicrobial activity against gram-positive and gram-negative pathogens.					
34571125	0	41	theme	quaternary	25:34	arg1	ammonium					36:43	quaternary ammonium	25:43	quaternary ammonium	25:43	Montmorillonite clay and quaternary ammonium silane-reinforced pullulan/agar-based nanocomposites and their properties for packaging applications.					
34571125	8	42	from	chain	1064:1068	arg1	QAS					1073:1075	QAS	1073:1075	QAS	1073:1075	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	7	43	dep	strength	933:940	arg1	The					921:923	The	921:923	The	921:923	The tensile strength and elongation at break of the composites were in the range of 23.8-39.7 MPa and 37.2-26.9%, respectively.					
34571125	2	44	theme	electron	476:483	arg1	microscopy					485:494	scanning electron microscopy	467:494	scanning electron microscopy	467:494	The Pullulan/agar/MMT clay/QAS nanocomposites were studied via Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analyses.					
34571125	9	45	theme	rheological	1376:1386	arg1	properties					1388:1397	the rheological properties	1372:1397	the rheological properties	1372:1397	The combination of Pullulan/agar/MMT clay/QAS had a synergistic effect on the rheological properties.					
34571125	5	46	theme	UV	749:750	arg1	transmittance					753:765	The ultraviolet (UV) transmittance	732:765	The ultraviolet (UV) transmittance of Pullulan/agar/MMT clay/QAS	732:795	The ultraviolet (UV) transmittance of Pullulan/agar/MMT clay/QAS was in the range of 91.4-79.8 at 600 nm.					
34571125	1	47	theme	Synergistic	147:157	arg1	combinations					159:170	Synergistic combinations	147:170	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	147:311	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	1	48	theme	quaternary	223:232	arg1	ammonium					234:241	quaternary ammonium	223:241	quaternary ammonium	223:241	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	5	49	theme	91.4-79.8	817:825	arg1	range					808:812	the range	804:812	the range of 91.4-79.8	804:825	The ultraviolet (UV) transmittance of Pullulan/agar/MMT clay/QAS was in the range of 91.4-79.8 at 600 nm.					
34571125	6	50	theme	MMT	911:913	arg1	clay					915:918	the MMT clay	907:918	the MMT clay	907:918	The thermal and mechanical properties were significantly improved by the MMT clay.					
34571125	8	51	theme	hydrophobic	1104:1114	arg1	nature					1116:1121	the hydrophobic nature	1100:1121	the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites	1100:1166	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	9	52	contain	had	1344:1346	arg2	effect					1362:1367	a synergistic effect	1348:1367	a synergistic effect	1348:1367	The combination of Pullulan/agar/MMT clay/QAS had a synergistic effect on the rheological properties.					
34571125	9	52	contain	had	1344:1346	arg1	combination					1302:1312	The combination	1298:1312	The combination of Pullulan/agar/MMT clay/QAS	1298:1342	The combination of Pullulan/agar/MMT clay/QAS had a synergistic effect on the rheological properties.					
34571125	1	53	theme	ammonium	234:241	arg1	combinations					159:170	Synergistic combinations	147:170	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	147:311	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	2	54	theme	infrared	444:451	arg1	spectroscopy					453:464	Fourier-transform infrared spectroscopy	426:464	Fourier-transform infrared spectroscopy	426:464	The Pullulan/agar/MMT clay/QAS nanocomposites were studied via Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction analyses.					
34571125	5	55	theme	Pullulan/agar/MMT	770:786	arg1	clay/QAS					788:795	Pullulan/agar/MMT clay/QAS	770:795	Pullulan/agar/MMT clay/QAS	770:795	The ultraviolet (UV) transmittance of Pullulan/agar/MMT clay/QAS was in the range of 91.4-79.8 at 600 nm.					
34571125	1	56	theme	silane	243:248	arg1	clay/QAS					281:288	Pullulan/agar/MMT clay/QAS	263:288	Pullulan/agar/MMT clay/QAS	263:288	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	1	56	theme	silane	243:248	arg1	nanocomposites					298:311	silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	243:311	silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	243:311	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	8	57	theme	clay	1148:1151	arg1	nanocomposites					1153:1166	the Pullulan/agar/MMT clay nanocomposites	1126:1166	the Pullulan/agar/MMT clay nanocomposites	1126:1166	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	3	58	theme	MMT	550:552	arg1	clay					554:557	MMT clay	550:557	MMT clay	550:557	The concentration of MMT clay played a very important role in the properties of the nanocomposites.					
34571125	0	59	theme	pullulan/agar-based	63:81	arg1	nanocomposites					83:96	pullulan/agar-based nanocomposites	63:96	pullulan/agar-based nanocomposites	63:96	Montmorillonite clay and quaternary ammonium silane-reinforced pullulan/agar-based nanocomposites and their properties for packaging applications.					
34571125	1	60	theme	simple	332:337	arg1	method					355:360	a simple, cost-effective method	330:360	a simple, cost-effective method	330:360	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	8	61	theme	Pullulan/agar/MMT	1130:1146	arg1	nanocomposites					1153:1166	the Pullulan/agar/MMT clay nanocomposites	1126:1166	the Pullulan/agar/MMT clay nanocomposites	1126:1166	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	3	62	theme	clay	554:557	arg1	concentration					533:545	The concentration	529:545	The concentration of MMT clay	529:557	The concentration of MMT clay played a very important role in the properties of the nanocomposites.					
34571125	10	63	theme	viscosity	1445:1453	arg1	modulus					1474:1480	the viscosity, storage, and loss modulus	1441:1480	the viscosity, storage, and loss modulus of the hydrogel composites	1441:1507	MMT clay and QAS significantly increased the viscosity, storage, and loss modulus of the hydrogel composites.					
34571125	11	64	theme	good	1585:1588	arg1	activity					1604:1611	good antimicrobial activity	1585:1611	good antimicrobial activity against gram-positive and gram-negative pathogens	1585:1661	With the addition of QAS, the Pullulan/agar/MMT clay nanocomposites showed good antimicrobial activity against gram-positive and gram-negative pathogens.					
34571125	1	65	theme	pullulan	175:182	arg1	combinations					159:170	Synergistic combinations	147:170	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	147:311	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	10	66	theme	MMT	1400:1402	arg1	clay					1404:1407	MMT clay	1400:1407	MMT clay	1400:1407	MMT clay and QAS significantly increased the viscosity, storage, and loss modulus of the hydrogel composites.					
34571125	5	67	theme	ultraviolet	736:746	arg1	transmittance					753:765	The ultraviolet (UV) transmittance	732:765	The ultraviolet (UV) transmittance of Pullulan/agar/MMT clay/QAS	732:795	The ultraviolet (UV) transmittance of Pullulan/agar/MMT clay/QAS was in the range of 91.4-79.8 at 600 nm.					
34571125	1	68	dep	silane	243:248	arg1	-based					255:260	-based	255:260	-based	255:260	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	1	68	dep	silane	243:248	arg1	QAS					251:253	QAS	251:253	QAS	251:253	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	4	69	theme	clay	726:729	arg1	addition					710:717	the addition	706:717	the addition of MMT clay	706:729	However, the transparency of the composite was not significantly affected by the addition of MMT clay.					
34571125	7	70	theme	%	1032:1032	arg1	range					996:1000	the range	992:1000	the range of 23.8-39.7 MPa and 37.2-26.9%	992:1032	The tensile strength and elongation at break of the composites were in the range of 23.8-39.7 MPa and 37.2-26.9%, respectively.					
34571125	1	71	theme	agar	185:188	arg1	combinations					159:170	Synergistic combinations	147:170	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites	147:311	Synergistic combinations of pullulan, agar, montmorillonite (MMT) clay, and quaternary ammonium silane (QAS)-based (Pullulan/agar/MMT clay/QAS) active nanocomposites were prepared by a simple, cost-effective method.					
34571125	8	72	theme	swelling	1270:1277	arg1	ratio					1279:1283	swelling ratio	1270:1283	swelling ratio (1837-836%)	1270:1295	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	8	72	theme	swelling	1270:1277	arg1	%					1294:1294	1837-836%	1286:1294	1837-836%	1286:1294	The long alkyl chain in QAS significantly improved the hydrophobic nature of the Pullulan/agar/MMT clay nanocomposites, impacting the contact angle (66.2-71.2°), water vapor permeability (3.17-2.20 × 10-9 g/m2 Pa·s), and swelling ratio (1837-836%).					
34571125	5	73	from	600 nm	830:835	arg1	range					808:812	the range	804:812	the range of 91.4-79.8	804:825	The ultraviolet (UV) transmittance of Pullulan/agar/MMT clay/QAS was in the range of 91.4-79.8 at 600 nm.					
34571125	7	74	theme	23.8-39.7 MPa	1005:1017	arg1	range					996:1000	the range	992:1000	the range of 23.8-39.7 MPa and 37.2-26.9%	992:1032	The tensile strength and elongation at break of the composites were in the range of 23.8-39.7 MPa and 37.2-26.9%, respectively.					
34571125	3	75	theme	important	573:581	arg1	role					583:586	a very important role	566:586	a very important role	566:586	The concentration of MMT clay played a very important role in the properties of the nanocomposites.					
34571125	7	76	theme	tensile	925:931	arg1	strength					933:940	tensile strength	925:940	tensile strength	925:940	The tensile strength and elongation at break of the composites were in the range of 23.8-39.7 MPa and 37.2-26.9%, respectively.					
34571125	4	77	theme	composite	662:670	arg1	transparency					642:653	the transparency	638:653	the transparency of the composite	638:670	However, the transparency of the composite was not significantly affected by the addition of MMT clay.					
34528737	8	0	theme	Estelite	940:947	arg1	Fill					954:957	Estelite Bulk Fill	940:957	Estelite Bulk Fill	940:957	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	9	1	contain	had	1042:1044	arg1	group					1036:1040	Z250 group	1031:1040	Z250 group	1031:1040	Among conventional composites, Z250 group had the highest nanohardness and elasticity modulus scores.					
34528737	9	1	contain	had	1042:1044	arg2	scores					1094:1099	the highest nanohardness and elasticity modulus scores	1046:1099	the highest nanohardness and elasticity modulus scores	1046:1099	Among conventional composites, Z250 group had the highest nanohardness and elasticity modulus scores.					
34528737	8	2	theme	test	987:990	arg1	groups					992:997	the test groups	983:997	the test groups	983:997	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	6	3	theme	Scanning	714:721	arg1	microscopy					732:741	Scanning electron microscopy	714:741	Scanning electron microscopy (SEM)	714:747	Scanning electron microscopy (SEM) was used to compare surface differences.					
34528737	6	3	theme	Scanning	714:721	arg1	SEM					744:746	SEM	744:746	SEM	744:746	Scanning electron microscopy (SEM) was used to compare surface differences.					
34528737	12	4	theme	Maximum	1394:1400	arg1	levels					1409:1414	Maximum height levels	1394:1414	Maximum height levels of samples	1394:1425	Maximum height levels of samples were recorded.					
34528737	9	5	theme	conventional	1006:1017	arg1	composites					1019:1028	conventional composites	1006:1028	conventional composites	1006:1028	Among conventional composites, Z250 group had the highest nanohardness and elasticity modulus scores.					
34528737	0	6	theme	probiotic	80:88	arg1	beverages					90:98	prebiotic and probiotic beverages	66:98	prebiotic and probiotic beverages	66:98	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.					
34528737	6	7	used	used	753:756	arg2	SEM					744:746	SEM	744:746	SEM	744:746	Scanning electron microscopy (SEM) was used to compare surface differences.					
34528737	6	7	used	used	753:756	arg2	microscopy					732:741	Scanning electron microscopy	714:741	Scanning electron microscopy (SEM)	714:747	Scanning electron microscopy (SEM) was used to compare surface differences.					
34528737	11	8	theme	bulk-fill	1279:1287	arg1	group					1289:1293	a bulk-fill group	1277:1293	a bulk-fill group	1277:1293	Reveal HD, as a bulk-fill group showed higher surface roughness and considerably lower nanohardness and elasticity modulus scores.					
34528737	10	9	theme	surface	1169:1175	arg1	roughness					1177:1185	the lowest surface roughness	1158:1185	the lowest surface roughness	1158:1185	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	14	10	theme	patients	1635:1642	arg1	restorations					1619:1630	the restorations	1615:1630	the restorations of patients who consume gut-friendly drinks regularly	1615:1684	Dentists may prefer Z250 as microhybrid and Estelite Bulk Fill Flow as bulk-fill composites for the restorations of patients who consume gut-friendly drinks regularly.					
34528737	5	11	theme	each	582:585	arg1	sample					572:577	One sample	568:577	One sample of each of the tested materials	568:609	One sample of each of the tested materials was examined under nanoindentation to evaluate the reduced elasticity modulus and nanohardness scores.					
34528737	10	12	from	immersion	1193:1201	arg1	beverages					1216:1224	probiotic beverages	1206:1224	probiotic beverages	1206:1224	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	7	13	dep	ANOVA	837:841	arg1	test					843:846	test	843:846	test	843:846	Data were analyzed statistically using one-way ANOVA test and the significance was set at p < .05.					
34528737	10	14	theme	lowest	1162:1167	arg1	roughness					1177:1185	the lowest surface roughness	1158:1185	the lowest surface roughness	1158:1185	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	5	15	theme	nanohardness	693:704	arg1	scores					706:711	nanohardness scores	693:711	nanohardness scores	693:711	One sample of each of the tested materials was examined under nanoindentation to evaluate the reduced elasticity modulus and nanohardness scores.					
34528737	8	16	theme	HRi	964:966	arg1	ENA					968:970	HRi ENA	964:970	HRi ENA	964:970	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	2	17	theme	samples	290:296	arg1	total					265:269	A total	263:269	A total of 420 disc-shaped samples (5 mm × 2 mm)	263:310	A total of 420 disc-shaped samples (5 mm × 2 mm) were prepared from resin-based composites.					
34528737	15	18	theme	useful	1751:1756	arg1	method					1758:1763	a useful method	1749:1763	a useful method for evaluating surface alterations and sensible comparisons	1749:1823	When there are various types of materials, nanoindentation is a useful method for evaluating surface alterations and sensible comparisons.					
34528737	11	19	theme	modulus	1378:1384	arg1	scores					1386:1391	considerably lower nanohardness and elasticity modulus scores	1331:1391	scores	1386:1391	Reveal HD, as a bulk-fill group showed higher surface roughness and considerably lower nanohardness and elasticity modulus scores.					
34528737	12	20	theme	height	1402:1407	arg1	levels					1409:1414	Maximum height levels	1394:1414	Maximum height levels of samples	1394:1425	Maximum height levels of samples were recorded.					
34528737	5	21	theme	tested	594:599	arg1	materials					601:609	the tested materials	590:609	the tested materials	590:609	One sample of each of the tested materials was examined under nanoindentation to evaluate the reduced elasticity modulus and nanohardness scores.					
34528737	10	22	contain	had	1154:1156	arg1	Flow					1149:1152	Estelite Bulk Fill Flow	1130:1152	Estelite Bulk Fill Flow	1130:1152	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	10	22	contain	had	1154:1156	arg2	roughness					1177:1185	the lowest surface roughness	1158:1185	the lowest surface roughness	1158:1185	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	2	23	theme	disc-shaped	278:288	arg1	samples					290:296	420 disc-shaped samples	274:296	420 disc-shaped samples (5 mm × 2 mm)	274:310	A total of 420 disc-shaped samples (5 mm × 2 mm) were prepared from resin-based composites.					
34528737	2	23	theme	disc-shaped	278:288	arg1	5 mm × 2 mm					299:309	5 mm × 2 mm	299:309	5 mm × 2 mm	299:309	A total of 420 disc-shaped samples (5 mm × 2 mm) were prepared from resin-based composites.					
34528737	15	24	theme	sensible	1804:1811	arg1	comparisons					1813:1823	sensible comparisons	1804:1823	sensible comparisons	1804:1823	When there are various types of materials, nanoindentation is a useful method for evaluating surface alterations and sensible comparisons.					
34528737	0	25	theme	nanoindentation	103:117	arg1	study					127:131	A nanoindentation and SEM study	101:131	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.	0:132	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.					
34528737	12	26	theme	samples	1419:1425	arg1	levels					1409:1414	Maximum height levels	1394:1414	Maximum height levels of samples	1394:1425	Maximum height levels of samples were recorded.					
34528737	15	27	theme	surface	1780:1786	arg1	alterations					1788:1798	surface alterations	1780:1798	surface alterations	1780:1798	When there are various types of materials, nanoindentation is a useful method for evaluating surface alterations and sensible comparisons.					
34528737	1	28	theme	surface	169:175	arg1	characteristics					177:191	the surface characteristics	165:191	the surface characteristics of various resin-based materials	165:224	The purpose was to investigate the surface characteristics of various resin-based materials by immersing in probiotic beverages.					
34528737	15	29	theme	various	1702:1708	arg1	types					1710:1714	various types	1702:1714	various types of materials	1702:1727	When there are various types of materials, nanoindentation is a useful method for evaluating surface alterations and sensible comparisons.					
34528737	14	30	theme	bulk-fill	1590:1598	arg1	composites					1600:1609	bulk-fill composites	1590:1609	bulk-fill composites for the restorations of patients who consume gut-friendly drinks regularly	1590:1684	Dentists may prefer Z250 as microhybrid and Estelite Bulk Fill Flow as bulk-fill composites for the restorations of patients who consume gut-friendly drinks regularly.					
34528737	14	30	theme	bulk-fill	1590:1598	arg1	Z250					1539:1542	Z250	1539:1542	Z250	1539:1542	Dentists may prefer Z250 as microhybrid and Estelite Bulk Fill Flow as bulk-fill composites for the restorations of patients who consume gut-friendly drinks regularly.					
34528737	6	31	theme	surface	769:775	arg1	differences					777:787	surface differences	769:787	surface differences	769:787	Scanning electron microscopy (SEM) was used to compare surface differences.					
34528737	13	32	from	voids	1462:1466	arg1	surfaces					1491:1498	the surfaces	1487:1498	the surfaces of test materials	1487:1516	SEM images revealed voids and microcracks on the surfaces of test materials.					
34528737	10	33	theme	nanohardness	1242:1253	arg1	values					1255:1260	the highest nanohardness values	1230:1260	the highest nanohardness values	1230:1260	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	15	34	theme	materials	1719:1727	arg1	types					1710:1714	various types	1702:1714	various types of materials	1702:1727	When there are various types of materials, nanoindentation is a useful method for evaluating surface alterations and sensible comparisons.					
34528737	11	35	theme	higher	1302:1307	arg1	roughness					1317:1325	higher surface roughness	1302:1325	higher surface roughness	1302:1325	Reveal HD, as a bulk-fill group showed higher surface roughness and considerably lower nanohardness and elasticity modulus scores.					
34528737	7	36	theme	one-way	829:835	arg1	ANOVA					837:841	one-way ANOVA test	829:846	one-way ANOVA test	829:846	Data were analyzed statistically using one-way ANOVA test and the significance was set at p < .05.					
34528737	11	37	theme	lower	1344:1348	arg1	nanohardness					1350:1361	considerably lower nanohardness and elasticity modulus scores	1331:1391	nanohardness	1350:1361	Reveal HD, as a bulk-fill group showed higher surface roughness and considerably lower nanohardness and elasticity modulus scores.					
34528737	0	38	theme	materials	26:34	arg1	Comparison					0:9	Comparison	0:9	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.	0:132	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.					
34528737	9	39	theme	nanohardness	1058:1069	arg1	scores					1094:1099	the highest nanohardness and elasticity modulus scores	1046:1099	the highest nanohardness and elasticity modulus scores	1046:1099	Among conventional composites, Z250 group had the highest nanohardness and elasticity modulus scores.					
34528737	1	40	theme	various	196:202	arg1	materials					216:224	various resin-based materials	196:224	various resin-based materials	196:224	The purpose was to investigate the surface characteristics of various resin-based materials by immersing in probiotic beverages.					
34528737	0	41	theme	restorative	14:24	arg1	materials					26:34	restorative materials	14:34	restorative materials	14:34	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.					
34528737	8	42	located	observed	922:929	arg1	Z250					934:937	Z250	934:937	Z250	934:937	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	8	42	located	observed	922:929	arg1	groups					992:997	the test groups	983:997	the test groups	983:997	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	8	42	located	observed	922:929	arg2	scores					910:915	The lowest roughness scores	889:915	The lowest roughness scores	889:915	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	8	42	located	observed	922:929	arg1	ENA					968:970	HRi ENA	964:970	HRi ENA	964:970	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	8	42	located	observed	922:929	arg1	most					975:978	most	975:978	most	975:978	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	8	42	located	observed	922:929	arg1	Fill					954:957	Estelite Bulk Fill	940:957	Estelite Bulk Fill	940:957	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	0	43	theme	SEM	123:125	arg1	study					127:131	A nanoindentation and SEM study	101:131	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.	0:132	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.					
34528737	9	44	theme	elasticity	1075:1084	arg1	scores					1094:1099	the highest nanohardness and elasticity modulus scores	1046:1099	the highest nanohardness and elasticity modulus scores	1046:1099	Among conventional composites, Z250 group had the highest nanohardness and elasticity modulus scores.					
34528737	8	45	theme	roughness	900:908	arg1	scores					910:915	The lowest roughness scores	889:915	The lowest roughness scores	889:915	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	1	46	theme	resin-based	204:214	arg1	materials					216:224	various resin-based materials	196:224	various resin-based materials	196:224	The purpose was to investigate the surface characteristics of various resin-based materials by immersing in probiotic beverages.					
34528737	0	47	theme	surface	40:46	arg1	alterations					48:58	surface alterations	40:58	surface alterations	40:58	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.					
34528737	15	48	dep	is	1746:1747	arg1	method					1758:1763	a useful method	1749:1763	a useful method for evaluating surface alterations and sensible comparisons	1749:1823	When there are various types of materials, nanoindentation is a useful method for evaluating surface alterations and sensible comparisons.					
34528737	11	49	dep	Reveal	1263:1268	arg1	as					1274:1275	as	1274:1275	as	1274:1275	Reveal HD, as a bulk-fill group showed higher surface roughness and considerably lower nanohardness and elasticity modulus scores.					
34528737	10	50	from	values	1255:1260	arg1	beverages					1216:1224	probiotic beverages	1206:1224	probiotic beverages	1206:1224	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	8	51	theme	lowest	893:898	arg1	scores					910:915	The lowest roughness scores	889:915	The lowest roughness scores	889:915	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	1	52	theme	materials	216:224	arg1	characteristics					177:191	the surface characteristics	165:191	the surface characteristics of various resin-based materials	165:224	The purpose was to investigate the surface characteristics of various resin-based materials by immersing in probiotic beverages.					
34528737	4	53	theme	Surface	512:518	arg1	roughness					520:528	Surface roughness	512:528	Surface roughness	512:528	Surface roughness was measured at baseline and 1 month.					
34528737	10	54	theme	Fill	1144:1147	arg1	Flow					1149:1152	Estelite Bulk Fill Flow	1130:1152	Estelite Bulk Fill Flow	1130:1152	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	9	55	theme	modulus	1086:1092	arg1	scores					1094:1099	the highest nanohardness and elasticity modulus scores	1046:1099	the highest nanohardness and elasticity modulus scores	1046:1099	Among conventional composites, Z250 group had the highest nanohardness and elasticity modulus scores.					
34528737	11	56	theme	surface	1309:1315	arg1	roughness					1317:1325	higher surface roughness	1302:1325	higher surface roughness	1302:1325	Reveal HD, as a bulk-fill group showed higher surface roughness and considerably lower nanohardness and elasticity modulus scores.					
34528737	10	57	theme	Estelite	1130:1137	arg1	Flow					1149:1152	Estelite Bulk Fill Flow	1130:1152	Estelite Bulk Fill Flow	1130:1152	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	11	58	theme	elasticity	1367:1376	arg1	scores					1386:1391	considerably lower nanohardness and elasticity modulus scores	1331:1391	scores	1386:1391	Reveal HD, as a bulk-fill group showed higher surface roughness and considerably lower nanohardness and elasticity modulus scores.					
34528737	13	59	from	microcracks	1472:1482	arg1	surfaces					1491:1498	the surfaces	1487:1498	the surfaces of test materials	1487:1516	SEM images revealed voids and microcracks on the surfaces of test materials.					
34528737	0	60	theme	alterations	48:58	arg1	Comparison					0:9	Comparison	0:9	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.	0:132	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.					
34528737	5	61	theme	reduced	662:668	arg1	modulus					681:687	the reduced elasticity modulus	658:687	the reduced elasticity modulus	658:687	One sample of each of the tested materials was examined under nanoindentation to evaluate the reduced elasticity modulus and nanohardness scores.					
34528737	14	62	theme	Fill	1577:1580	arg1	Flow					1582:1585	Estelite Bulk Fill Flow	1563:1585	Estelite Bulk Fill Flow	1563:1585	Dentists may prefer Z250 as microhybrid and Estelite Bulk Fill Flow as bulk-fill composites for the restorations of patients who consume gut-friendly drinks regularly.					
34528737	3	63	theme	artificial	483:492	arg1	control					502:508	control	502:508	control	502:508	Samples were divided into four groups and immersed for 10 min/day for 1 month in either a probiotic sachet, kefir, kombucha, or artificial saliva (control).					
34528737	3	63	theme	artificial	483:492	arg1	saliva					494:499	artificial saliva	483:499	artificial saliva (control)	483:509	Samples were divided into four groups and immersed for 10 min/day for 1 month in either a probiotic sachet, kefir, kombucha, or artificial saliva (control).					
34528737	10	64	theme	probiotic	1206:1214	arg1	beverages					1216:1224	probiotic beverages	1206:1224	probiotic beverages	1206:1224	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	10	65	theme	bulk-fill	1108:1116	arg1	composites					1118:1127	bulk-fill composites	1108:1127	bulk-fill composites	1108:1127	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	5	66	theme	elasticity	670:679	arg1	modulus					681:687	the reduced elasticity modulus	658:687	the reduced elasticity modulus	658:687	One sample of each of the tested materials was examined under nanoindentation to evaluate the reduced elasticity modulus and nanohardness scores.					
34528737	9	67	theme	highest	1050:1056	arg1	scores					1094:1099	the highest nanohardness and elasticity modulus scores	1046:1099	the highest nanohardness and elasticity modulus scores	1046:1099	Among conventional composites, Z250 group had the highest nanohardness and elasticity modulus scores.					
34528737	2	68	theme	resin-based	331:341	arg1	composites					343:352	resin-based composites	331:352	resin-based composites	331:352	A total of 420 disc-shaped samples (5 mm × 2 mm) were prepared from resin-based composites.					
34528737	8	69	theme	Bulk	949:952	arg1	Fill					954:957	Estelite Bulk Fill	940:957	Estelite Bulk Fill	940:957	The lowest roughness scores were observed in Z250, Estelite Bulk Fill, and HRi ENA in most of the test groups.					
34528737	14	70	theme	Bulk	1572:1575	arg1	Flow					1582:1585	Estelite Bulk Fill Flow	1563:1585	Estelite Bulk Fill Flow	1563:1585	Dentists may prefer Z250 as microhybrid and Estelite Bulk Fill Flow as bulk-fill composites for the restorations of patients who consume gut-friendly drinks regularly.					
34528737	14	71	theme	gut-friendly	1656:1667	arg1	drinks					1669:1674	gut-friendly drinks	1656:1674	gut-friendly drinks	1656:1674	Dentists may prefer Z250 as microhybrid and Estelite Bulk Fill Flow as bulk-fill composites for the restorations of patients who consume gut-friendly drinks regularly.					
34528737	13	72	theme	test	1503:1506	arg1	materials					1508:1516	test materials	1503:1516	test materials	1503:1516	SEM images revealed voids and microcracks on the surfaces of test materials.					
34528737	14	73	theme	Estelite	1563:1570	arg1	Flow					1582:1585	Estelite Bulk Fill Flow	1563:1585	Estelite Bulk Fill Flow	1563:1585	Dentists may prefer Z250 as microhybrid and Estelite Bulk Fill Flow as bulk-fill composites for the restorations of patients who consume gut-friendly drinks regularly.					
34528737	13	74	theme	SEM	1442:1444	arg1	images					1446:1451	SEM images	1442:1451	SEM images	1442:1451	SEM images revealed voids and microcracks on the surfaces of test materials.					
34528737	13	75	theme	materials	1508:1516	arg1	surfaces					1491:1498	the surfaces	1487:1498	the surfaces of test materials	1487:1516	SEM images revealed voids and microcracks on the surfaces of test materials.					
34528737	3	76	theme	probiotic	445:453	arg1	sachet					455:460	a probiotic sachet	443:460	a probiotic sachet	443:460	Samples were divided into four groups and immersed for 10 min/day for 1 month in either a probiotic sachet, kefir, kombucha, or artificial saliva (control).					
34528737	1	77	theme	probiotic	242:250	arg1	beverages					252:260	probiotic beverages	242:260	probiotic beverages	242:260	The purpose was to investigate the surface characteristics of various resin-based materials by immersing in probiotic beverages.					
34528737	0	78	theme	prebiotic	66:74	arg1	beverages					90:98	prebiotic and probiotic beverages	66:98	prebiotic and probiotic beverages	66:98	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.					
34528737	0	79	dep	Comparison	0:9	arg1	study					127:131	A nanoindentation and SEM study	101:131	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.	0:132	Comparison of restorative materials and surface alterations after prebiotic and probiotic beverages: A nanoindentation and SEM study.					
34528737	6	80	theme	electron	723:730	arg1	microscopy					732:741	Scanning electron microscopy	714:741	Scanning electron microscopy (SEM)	714:747	Scanning electron microscopy (SEM) was used to compare surface differences.					
34528737	6	80	theme	electron	723:730	arg1	SEM					744:746	SEM	744:746	SEM	744:746	Scanning electron microscopy (SEM) was used to compare surface differences.					
34528737	10	81	theme	highest	1234:1240	arg1	values					1255:1260	the highest nanohardness values	1230:1260	the highest nanohardness values	1230:1260	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
34528737	10	82	theme	Bulk	1139:1142	arg1	Flow					1149:1152	Estelite Bulk Fill Flow	1130:1152	Estelite Bulk Fill Flow	1130:1152	Among bulk-fill composites, Estelite Bulk Fill Flow had the lowest surface roughness after immersion in probiotic beverages and the highest nanohardness values.					
32918522	1	0	theme	small	237:241	arg1	SI					254:255	SI	254:255	SI	254:255	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	1	0	theme	small	237:241	arg1	intestine					243:251	small intestine	237:251	human small intestine (SI) microbiota	231:267	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	9	1	theme	fibers	1215:1220	arg1	Degradation					1190:1200	Degradation	1190:1200	Degradation of different fibers	1190:1220	Degradation of different fibers result in production of mainly acetate, and changed microbiota composition over time.					
32918522	2	2	from	fermentation	301:312	arg1	SI					351:352	the distal SI	340:352	the distal SI of humans and pigs	340:371	A few previous studies hint at fermentation of some dietary fibers in the distal SI of humans and pigs.					
32918522	7	3	used	utilized	1044:1051	arg2	fructo-oligosaccharides					983:1005	31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides	935:1005	31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8)	935:1038	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	2	4	theme	previous	276:283	arg1	studies					285:291	A few previous studies	270:291	A few previous studies	270:291	A few previous studies hint at fermentation of some dietary fibers in the distal SI of humans and pigs.					
32918522	0	5	theme	Fiber	108:112	arg1	Type					100:103	the Type	96:103	the Type of Fiber and Subject	96:124	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota Depend on the Type of Fiber and Subject.					
32918522	9	6	theme	acetate	1253:1259	arg1	production					1232:1241	production	1232:1241	production of mainly acetate	1232:1259	Degradation of different fibers result in production of mainly acetate, and changed microbiota composition over time.					
32918522	1	7	theme	effects	187:193	arg1	investigation					163:175	the investigation	159:175	the investigation of health effects of dietary fibers	159:211	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	1	7	theme	effects	187:193	arg1	SCOPE					127:131	SCOPE	127:131	SCOPE An underexplored topic	127:154	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	0	8	theme	Subject	118:124	arg1	Type					100:103	the Type	96:103	the Type of Fiber and Subject	96:124	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota Depend on the Type of Fiber and Subject.					
32918522	1	9	theme	dietary	198:204	arg1	fibers					206:211	dietary fibers	198:211	dietary fibers	198:211	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	12	10	theme	based	1667:1671	arg1	strategies					1673:1682	personalized nutritional fiber based strategies	1636:1682	personalized nutritional fiber based strategies	1636:1682	Fiber degradation kinetics and microbiota responses are subject dependent, therefore personalized nutritional fiber based strategies are required.					
32918522	9	11	theme	microbiota	1274:1283	arg1	composition					1285:1295	microbiota composition	1274:1295	microbiota composition	1274:1295	Degradation of different fibers result in production of mainly acetate, and changed microbiota composition over time.					
32918522	7	12	dep	%	941:941	arg1	%					981:981	-89%	978:981	-89%	978:981	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	7	13	theme	polymerization	1018:1031	arg1	degree					1008:1013	degree	1008:1013	degree of polymerization DP4-8	1008:1037	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	10	14	dep	CONCLUSION	1308:1317	arg1	microbiota					1328:1337	Human SI microbiota	1319:1337	CONCLUSION Human SI microbiota	1308:1337	CONCLUSION Human SI microbiota have hydrolytic potential for prebiotic galacto- and fructo-oligosaccharides.					
32918522	10	15	theme	hydrolytic	1344:1353	arg1	potential					1355:1363	hydrolytic potential	1344:1363	hydrolytic potential for prebiotic galacto- and fructo-oligosaccharides	1344:1414	CONCLUSION Human SI microbiota have hydrolytic potential for prebiotic galacto- and fructo-oligosaccharides.					
32918522	5	16	theme	metabolites	750:760	arg1	formation					727:735	formation	727:735	formation of bacterial metabolites	727:760	Fiber degradation products, formation of bacterial metabolites, and microbiota composition are determined over time.					
32918522	5	16	theme	metabolites	750:760	arg1	products					717:724	Fiber degradation products	699:724	Fiber degradation products	699:724	Fiber degradation products, formation of bacterial metabolites, and microbiota composition are determined over time.					
32918522	5	16	theme	metabolites	750:760	arg1	composition					778:788	microbiota composition	767:788	microbiota composition	767:788	Fiber degradation products, formation of bacterial metabolites, and microbiota composition are determined over time.					
32918522	4	17	dep	METHODS	492:498	arg1	Fructans					512:519	Fructans	512:519	Fructans	512:519	METHODS AND RESULTS Fructans, galacto-oligosaccharides, lemon pectins, and isomalto/malto-polysaccharides are subjected to in vitro batch fermentations inoculated with ileostomy effluent from five subjects.					
32918522	12	18	theme	nutritional	1649:1659	arg1	strategies					1673:1682	personalized nutritional fiber based strategies	1636:1682	personalized nutritional fiber based strategies	1636:1682	Fiber degradation kinetics and microbiota responses are subject dependent, therefore personalized nutritional fiber based strategies are required.					
32918522	7	19	theme	%	977:977	arg1	fructo-oligosaccharides					983:1005	31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides	935:1005	31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8)	935:1038	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	7	20	dep	fructo-oligosaccharides	983:1005	arg1	degree					1008:1013	degree	1008:1013	degree of polymerization DP4-8	1008:1037	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	0	21	theme	Fibers	42:47	arg1	Kinetics					13:20	Fermentation Kinetics	0:20	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota	0:84	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota Depend on the Type of Fiber and Subject.					
32918522	0	22	theme	Intestinal	64:73	arg1	Microbiota					75:84	Human Small Intestinal Microbiota	52:84	Human Small Intestinal Microbiota	52:84	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota Depend on the Type of Fiber and Subject.					
32918522	2	23	theme	distal	344:349	arg1	SI					351:352	the distal SI	340:352	the distal SI of humans and pigs	340:371	A few previous studies hint at fermentation of some dietary fibers in the distal SI of humans and pigs.					
32918522	0	24	theme	Fermentation	0:11	arg1	Kinetics					13:20	Fermentation Kinetics	0:20	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota	0:84	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota Depend on the Type of Fiber and Subject.					
32918522	7	25	theme	fermentation	921:932	arg1	5h					915:916	5h	915:916	5h of fermentation	915:932	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	1	26	dep	SCOPE	127:131	arg1	topic					150:154	An underexplored topic	133:154	SCOPE An underexplored topic	127:154	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	11	27	theme	fermentation	1532:1543	arg1	rate					1545:1548	slow fermentation rate	1527:1548	slow fermentation rate	1527:1548	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	3	28	theme	microbiota	405:414	arg1	potential					383:391	the potential	379:391	the potential of human SI microbiota to degrade dietary fibers and produce metabolites in vitro	379:473	Here the potential of human SI microbiota to degrade dietary fibers and produce metabolites in vitro is investigated.					
32918522	1	29	theme	microbiota	258:267	arg1	modulation					217:226	modulation	217:226	modulation of human small intestine (SI) microbiota	217:267	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	6	30	theme	SI	881:882	arg1	microbiota					884:893	the SI microbiota	877:893	the SI microbiota of all subjects	877:909	Galacto- and fructo-oligosaccharides are rapidly utilized by the SI microbiota of all subjects.					
32918522	3	31	theme	human	396:400	arg1	microbiota					405:414	human SI microbiota	396:414	human SI microbiota	396:414	Here the potential of human SI microbiota to degrade dietary fibers and produce metabolites in vitro is investigated.					
32918522	0	32	theme	Dietary	34:40	arg1	Fibers					42:47	Selected Dietary Fibers	25:47	Selected Dietary Fibers	25:47	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota Depend on the Type of Fiber and Subject.					
32918522	8	33	theme	DP ≥ 10	1098:1104	arg1	Breakdown					1054:1062	Breakdown	1054:1062	Breakdown of fructo-oligosaccharides/inulin DP ≥ 10, lemon pectin, and iso-malto/maltopolysaccharides	1054:1154	Breakdown of fructo-oligosaccharides/inulin DP ≥ 10, lemon pectin, and iso-malto/maltopolysaccharides only started after 7h incubation.					
32918522	4	34	dep	in	615:616	arg1	vitro					618:622	vitro	618:622	vitro	618:622	METHODS AND RESULTS Fructans, galacto-oligosaccharides, lemon pectins, and isomalto/malto-polysaccharides are subjected to in vitro batch fermentations inoculated with ileostomy effluent from five subjects.					
32918522	0	35	theme	Small	58:62	arg1	Microbiota					75:84	Human Small Intestinal Microbiota	52:84	Human Small Intestinal Microbiota	52:84	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota Depend on the Type of Fiber and Subject.					
32918522	8	36	theme	lemon	1107:1111	arg1	pectin					1113:1118	lemon pectin	1107:1118	lemon pectin	1107:1118	Breakdown of fructo-oligosaccharides/inulin DP ≥ 10, lemon pectin, and iso-malto/maltopolysaccharides only started after 7h incubation.					
32918522	1	37	theme	fibers	206:211	arg1	effects					187:193	health effects	180:193	health effects of dietary fibers	180:211	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	12	38	theme	degradation	1557:1567	arg1	kinetics					1569:1576	Fiber degradation kinetics	1551:1576	Fiber degradation kinetics	1551:1576	Fiber degradation kinetics and microbiota responses are subject dependent, therefore personalized nutritional fiber based strategies are required.					
32918522	11	39	theme	higher	1434:1439	arg1	weight					1451:1456	higher molecular weight	1434:1456	the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides	1430:1520	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	2	40	theme	humans	357:362	arg1	SI					351:352	the distal SI	340:352	the distal SI of humans and pigs	340:371	A few previous studies hint at fermentation of some dietary fibers in the distal SI of humans and pigs.					
32918522	3	41	theme	in	466:467	arg1	metabolites					454:464	metabolites	454:464	metabolites in vitro	454:473	Here the potential of human SI microbiota to degrade dietary fibers and produce metabolites in vitro is investigated.					
32918522	4	42	from	subjects	689:696	arg1	effluent					670:677	ileostomy effluent	660:677	ileostomy effluent from five subjects	660:696	METHODS AND RESULTS Fructans, galacto-oligosaccharides, lemon pectins, and isomalto/malto-polysaccharides are subjected to in vitro batch fermentations inoculated with ileostomy effluent from five subjects.					
32918522	8	43	theme	iso-malto/maltopolysaccharides	1125:1154	arg1	Breakdown					1054:1062	Breakdown	1054:1062	Breakdown of fructo-oligosaccharides/inulin DP ≥ 10, lemon pectin, and iso-malto/maltopolysaccharides	1054:1154	Breakdown of fructo-oligosaccharides/inulin DP ≥ 10, lemon pectin, and iso-malto/maltopolysaccharides only started after 7h incubation.					
32918522	2	44	theme	dietary	322:328	arg1	fibers					330:335	some dietary fibers	317:335	some dietary fibers	317:335	A few previous studies hint at fermentation of some dietary fibers in the distal SI of humans and pigs.					
32918522	1	45	theme	human	231:235	arg1	microbiota					258:267	human small intestine (SI) microbiota	231:267	human small intestine (SI) microbiota	231:267	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	9	46	theme	different	1205:1213	arg1	fibers					1215:1220	different fibers	1205:1220	different fibers	1205:1220	Degradation of different fibers result in production of mainly acetate, and changed microbiota composition over time.					
32918522	1	47	theme	intestine	243:251	arg1	microbiota					258:267	human small intestine (SI) microbiota	231:267	human small intestine (SI) microbiota	231:267	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	8	48	theme	7h	1175:1176	arg1	incubation					1178:1187	7h incubation	1175:1187	7h incubation	1175:1187	Breakdown of fructo-oligosaccharides/inulin DP ≥ 10, lemon pectin, and iso-malto/maltopolysaccharides only started after 7h incubation.					
32918522	6	49	theme	subjects	902:909	arg1	microbiota					884:893	the SI microbiota	877:893	the SI microbiota of all subjects	877:909	Galacto- and fructo-oligosaccharides are rapidly utilized by the SI microbiota of all subjects.					
32918522	4	50	theme	lemon	548:552	arg1	pectins					554:560	lemon pectins	548:560	lemon pectins	548:560	METHODS AND RESULTS Fructans, galacto-oligosaccharides, lemon pectins, and isomalto/malto-polysaccharides are subjected to in vitro batch fermentations inoculated with ileostomy effluent from five subjects.					
32918522	11	51	theme	weight	1451:1456	arg1	fibers					1458:1463	the higher molecular weight fibers	1430:1463	the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides	1430:1520	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	11	51	theme	weight	1451:1456	arg1	inulin					1465:1470	inulin	1465:1470	inulin	1465:1470	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	11	51	theme	weight	1451:1456	arg1	pectin					1479:1484	lemon pectin	1473:1484	lemon pectin	1473:1484	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	11	51	theme	weight	1451:1456	arg1	iso-malto/maltopolysaccharides					1491:1520	iso-malto/maltopolysaccharides	1491:1520	iso-malto/maltopolysaccharides	1491:1520	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	4	52	theme	batch	624:628	arg1	fermentations					630:642	in vitro batch fermentations	615:642	in vitro batch fermentations inoculated with ileostomy effluent from five subjects	615:696	METHODS AND RESULTS Fructans, galacto-oligosaccharides, lemon pectins, and isomalto/malto-polysaccharides are subjected to in vitro batch fermentations inoculated with ileostomy effluent from five subjects.					
32918522	2	53	theme	few	272:274	arg1	studies					285:291	A few previous studies	270:291	A few previous studies	270:291	A few previous studies hint at fermentation of some dietary fibers in the distal SI of humans and pigs.					
32918522	1	54	theme	health	180:185	arg1	effects					187:193	health effects	180:193	health effects of dietary fibers	180:211	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	5	55	theme	Fiber	699:703	arg1	products					717:724	Fiber degradation products	699:724	Fiber degradation products	699:724	Fiber degradation products, formation of bacterial metabolites, and microbiota composition are determined over time.					
32918522	4	56	theme	in	615:616	arg1	fermentations					630:642	in vitro batch fermentations	615:642	in vitro batch fermentations inoculated with ileostomy effluent from five subjects	615:696	METHODS AND RESULTS Fructans, galacto-oligosaccharides, lemon pectins, and isomalto/malto-polysaccharides are subjected to in vitro batch fermentations inoculated with ileostomy effluent from five subjects.					
32918522	11	57	dep	fibers	1458:1463	arg1	fibers					1458:1463	the higher molecular weight fibers	1430:1463	the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides	1430:1520	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	11	57	dep	fibers	1458:1463	arg1	inulin					1465:1470	inulin	1465:1470	inulin	1465:1470	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	11	57	dep	fibers	1458:1463	arg1	pectin					1479:1484	lemon pectin	1473:1484	lemon pectin	1473:1484	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	11	57	dep	fibers	1458:1463	arg1	iso-malto/maltopolysaccharides					1491:1520	iso-malto/maltopolysaccharides	1491:1520	iso-malto/maltopolysaccharides	1491:1520	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	12	58	theme	personalized	1636:1647	arg1	strategies					1673:1682	personalized nutritional fiber based strategies	1636:1682	personalized nutritional fiber based strategies	1636:1682	Fiber degradation kinetics and microbiota responses are subject dependent, therefore personalized nutritional fiber based strategies are required.					
32918522	5	59	theme	bacterial	740:748	arg1	metabolites					750:760	bacterial metabolites	740:760	bacterial metabolites	740:760	Fiber degradation products, formation of bacterial metabolites, and microbiota composition are determined over time.					
32918522	12	60	theme	fiber	1661:1665	arg1	strategies					1673:1682	personalized nutritional fiber based strategies	1636:1682	personalized nutritional fiber based strategies	1636:1682	Fiber degradation kinetics and microbiota responses are subject dependent, therefore personalized nutritional fiber based strategies are required.					
32918522	4	61	theme	ileostomy	660:668	arg1	effluent					670:677	ileostomy effluent	660:677	ileostomy effluent from five subjects	660:696	METHODS AND RESULTS Fructans, galacto-oligosaccharides, lemon pectins, and isomalto/malto-polysaccharides are subjected to in vitro batch fermentations inoculated with ileostomy effluent from five subjects.					
32918522	1	62	theme	underexplored	136:148	arg1	topic					150:154	An underexplored topic	133:154	SCOPE An underexplored topic	127:154	SCOPE An underexplored topic is the investigation of health effects of dietary fibers via modulation of human small intestine (SI) microbiota.					
32918522	7	63	theme	galacto-oligosaccharides	946:969	arg1	%					977:977	29%	975:977	29%	975:977	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	7	63	theme	galacto-oligosaccharides	946:969	arg1	%					941:941	31%-82%	935:941	31%-82% of galacto-oligosaccharides	935:969	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	7	63	theme	galacto-oligosaccharides	946:969	arg1	galacto-oligosaccharides					946:969	galacto-oligosaccharides	946:969	galacto-oligosaccharides	946:969	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	10	64	theme	SI	1325:1326	arg1	microbiota					1328:1337	Human SI microbiota	1319:1337	CONCLUSION Human SI microbiota	1308:1337	CONCLUSION Human SI microbiota have hydrolytic potential for prebiotic galacto- and fructo-oligosaccharides.					
32918522	10	65	contain	have	1339:1342	arg2	potential					1355:1363	hydrolytic potential	1344:1363	hydrolytic potential for prebiotic galacto- and fructo-oligosaccharides	1344:1414	CONCLUSION Human SI microbiota have hydrolytic potential for prebiotic galacto- and fructo-oligosaccharides.					
32918522	10	65	contain	have	1339:1342	arg1	CONCLUSION					1308:1317	CONCLUSION Human SI microbiota	1308:1337	CONCLUSION Human SI microbiota	1308:1337	CONCLUSION Human SI microbiota have hydrolytic potential for prebiotic galacto- and fructo-oligosaccharides.					
32918522	7	66	theme	%	941:941	arg1	fructo-oligosaccharides					983:1005	31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides	935:1005	31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8)	935:1038	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	11	67	theme	lemon	1473:1477	arg1	fibers					1458:1463	the higher molecular weight fibers	1430:1463	the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides	1430:1520	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	11	67	theme	lemon	1473:1477	arg1	pectin					1479:1484	lemon pectin	1473:1484	lemon pectin	1473:1484	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	7	68	theme	%	937:937	arg1	%					941:941	31%-82%	935:941	31%-82% of galacto-oligosaccharides	935:969	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	7	68	theme	%	937:937	arg1	galacto-oligosaccharides					946:969	galacto-oligosaccharides	946:969	galacto-oligosaccharides	946:969	At 5h of fermentation, 31%-82% of galacto-oligosaccharides and 29%-89% fructo-oligosaccharides (degree of polymerization DP4-8) are utilized.					
32918522	11	69	theme	slow	1527:1530	arg1	rate					1545:1548	slow fermentation rate	1527:1548	slow fermentation rate	1527:1548	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	0	70	theme	Selected	25:32	arg1	Fibers					42:47	Selected Dietary Fibers	25:47	Selected Dietary Fibers	25:47	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota Depend on the Type of Fiber and Subject.					
32918522	8	71	theme	fructo-oligosaccharides/inulin	1067:1096	arg1	DP ≥ 10					1098:1104	fructo-oligosaccharides/inulin DP ≥ 10	1067:1104	fructo-oligosaccharides/inulin DP ≥ 10	1067:1104	Breakdown of fructo-oligosaccharides/inulin DP ≥ 10, lemon pectin, and iso-malto/maltopolysaccharides only started after 7h incubation.					
32918522	3	72	dep	in	466:467	arg1	vitro					469:473	vitro	469:473	vitro	469:473	Here the potential of human SI microbiota to degrade dietary fibers and produce metabolites in vitro is investigated.					
32918522	2	73	theme	pigs	368:371	arg1	SI					351:352	the distal SI	340:352	the distal SI of humans and pigs	340:371	A few previous studies hint at fermentation of some dietary fibers in the distal SI of humans and pigs.					
32918522	5	74	theme	microbiota	767:776	arg1	composition					778:788	microbiota composition	767:788	microbiota composition	767:788	Fiber degradation products, formation of bacterial metabolites, and microbiota composition are determined over time.					
32918522	10	75	theme	Human	1319:1323	arg1	microbiota					1328:1337	Human SI microbiota	1319:1337	CONCLUSION Human SI microbiota	1308:1337	CONCLUSION Human SI microbiota have hydrolytic potential for prebiotic galacto- and fructo-oligosaccharides.					
32918522	6	76	used	utilized	865:872	arg2	Galacto-					816:823	Galacto-	816:823	Galacto-	816:823	Galacto- and fructo-oligosaccharides are rapidly utilized by the SI microbiota of all subjects.					
32918522	6	76	used	utilized	865:872	arg2	fructo-oligosaccharides					829:851	fructo-oligosaccharides	829:851	fructo-oligosaccharides	829:851	Galacto- and fructo-oligosaccharides are rapidly utilized by the SI microbiota of all subjects.					
32918522	3	77	theme	SI	402:403	arg1	microbiota					405:414	human SI microbiota	396:414	human SI microbiota	396:414	Here the potential of human SI microbiota to degrade dietary fibers and produce metabolites in vitro is investigated.					
32918522	0	78	theme	Human	52:56	arg1	Microbiota					75:84	Human Small Intestinal Microbiota	52:84	Human Small Intestinal Microbiota	52:84	Fermentation Kinetics of Selected Dietary Fibers by Human Small Intestinal Microbiota Depend on the Type of Fiber and Subject.					
32918522	8	79	theme	pectin	1113:1118	arg1	Breakdown					1054:1062	Breakdown	1054:1062	Breakdown of fructo-oligosaccharides/inulin DP ≥ 10, lemon pectin, and iso-malto/maltopolysaccharides	1054:1154	Breakdown of fructo-oligosaccharides/inulin DP ≥ 10, lemon pectin, and iso-malto/maltopolysaccharides only started after 7h incubation.					
32918522	12	80	theme	microbiota	1582:1591	arg1	responses					1593:1601	microbiota responses	1582:1601	microbiota responses	1582:1601	Fiber degradation kinetics and microbiota responses are subject dependent, therefore personalized nutritional fiber based strategies are required.					
32918522	5	81	theme	degradation	705:715	arg1	products					717:724	Fiber degradation products	699:724	Fiber degradation products	699:724	Fiber degradation products, formation of bacterial metabolites, and microbiota composition are determined over time.					
32918522	10	82	theme	prebiotic	1369:1377	arg1	galacto-					1379:1386	prebiotic galacto-	1369:1386	prebiotic galacto-	1369:1386	CONCLUSION Human SI microbiota have hydrolytic potential for prebiotic galacto- and fructo-oligosaccharides.					
32918522	3	83	theme	dietary	427:433	arg1	fibers					435:440	dietary fibers	427:440	dietary fibers	427:440	Here the potential of human SI microbiota to degrade dietary fibers and produce metabolites in vitro is investigated.					
32918522	12	84	theme	Fiber	1551:1555	arg1	kinetics					1569:1576	Fiber degradation kinetics	1551:1576	Fiber degradation kinetics	1551:1576	Fiber degradation kinetics and microbiota responses are subject dependent, therefore personalized nutritional fiber based strategies are required.					
32918522	11	85	theme	molecular	1441:1449	arg1	weight					1451:1456	higher molecular weight	1434:1456	the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides	1430:1520	In contrast, the higher molecular weight fibers inulin, lemon pectin, and iso-malto/maltopolysaccharides show slow fermentation rate.					
32918522	2	86	theme	fibers	330:335	arg1	fermentation					301:312	fermentation	301:312	fermentation of some dietary fibers in the distal SI of humans and pigs	301:371	A few previous studies hint at fermentation of some dietary fibers in the distal SI of humans and pigs.					
32616810	8	0	theme	I	1222:1222	arg1	constituents					1242:1253	the type I primary cell wall constituents	1213:1253	the type I primary cell wall constituents	1213:1253	Thus, the machinery to generate the type I primary cell wall constituents is completely established and operates.					
32616810	7	1	with	Rhamnogalacturonans-I	1048:1068	arg1	side-chains					1079:1089	the side-chains	1075:1089	the side-chains of branched 1,4-galactan and arabinan	1075:1127	Rhamnogalacturonans-I with the side-chains of branched 1,4-galactan and arabinan persisted in cell walls throughout the development.					
32616810	4	2	theme	wall	709:712	arg1	formation					714:722	cell wall formation	704:722	cell wall formation	704:722	Specific patterns of cell wall formation differentiate the initiation, realization and cessation of elongation growth.					
32616810	1	3	theme	wall	134:137	arg1	polysaccharides					139:153	cell wall polysaccharides	129:153	cell wall polysaccharides	129:153	The dynamics of cell wall polysaccharides may modulate the cell wall mechanics and thus control the expansion growth of plant cells.					
32616810	10	4	theme	cell	1550:1553	arg1	growth					1555:1560	grass cell growth	1544:1560	grass cell growth	1544:1560	These findings widen the number of jigsaw pieces which should be put together to solve the puzzle of grass cell growth.					
32616810	8	5	theme	type	1217:1220	arg1	constituents					1242:1253	the type I primary cell wall constituents	1213:1253	the type I primary cell wall constituents	1213:1253	Thus, the machinery to generate the type I primary cell wall constituents is completely established and operates.					
32616810	10	6	theme	grass	1544:1548	arg1	growth					1555:1560	grass cell growth	1544:1560	grass cell growth	1544:1560	These findings widen the number of jigsaw pieces which should be put together to solve the puzzle of grass cell growth.					
32616810	1	7	theme	polysaccharides	139:153	arg1	dynamics					117:124	The dynamics	113:124	The dynamics of cell wall polysaccharides	113:153	The dynamics of cell wall polysaccharides may modulate the cell wall mechanics and thus control the expansion growth of plant cells.					
32616810	1	8	theme	plant	233:237	arg1	cells					239:243	plant cells	233:243	plant cells	233:243	The dynamics of cell wall polysaccharides may modulate the cell wall mechanics and thus control the expansion growth of plant cells.					
32616810	8	9	theme	cell	1232:1235	arg1	constituents					1242:1253	the type I primary cell wall constituents	1213:1253	the type I primary cell wall constituents	1213:1253	Thus, the machinery to generate the type I primary cell wall constituents is completely established and operates.					
32616810	9	10	theme	active	1416:1421	arg1	elongation					1431:1440	active or late elongation	1416:1440	active or late elongation	1416:1440	The expression of glycosyltransferases responsible for mixed-linkage glucan and glucuronoarabinoxylan synthesis peaks at active or late elongation.					
32616810	10	11	theme	jigsaw	1478:1483	arg1	pieces					1485:1490	jigsaw pieces	1478:1490	jigsaw pieces which should be put together to solve the puzzle of grass cell growth	1478:1560	These findings widen the number of jigsaw pieces which should be put together to solve the puzzle of grass cell growth.					
32616810	4	12	theme	elongation	783:792	arg1	growth					794:799	elongation growth	783:799	elongation growth	783:799	Specific patterns of cell wall formation differentiate the initiation, realization and cessation of elongation growth.					
32616810	1	13	theme	cells	239:243	arg1	growth					223:228	the expansion growth	209:228	the expansion growth of plant cells	209:243	The dynamics of cell wall polysaccharides may modulate the cell wall mechanics and thus control the expansion growth of plant cells.					
32616810	8	14	theme	primary	1224:1230	arg1	constituents					1242:1253	the type I primary cell wall constituents	1213:1253	the type I primary cell wall constituents	1213:1253	Thus, the machinery to generate the type I primary cell wall constituents is completely established and operates.					
32616810	3	15	theme	extensive	542:550	arg1	analysis					572:579	extensive immunohistochemical analysis	542:579	extensive immunohistochemical analysis	542:579	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	5	16	theme	type	874:877	arg1	mixture					863:869	a mixture	861:869	a mixture of type I and type II specific polysaccharides	861:916	Cell walls of meristem and early elongation zone represent a mixture of type I and type II specific polysaccharides.					
32616810	0	17	theme	cell	101:104	arg1	walls					106:110	primary cell walls	93:110	primary cell walls	93:110	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	9	18	theme	late	1426:1429	arg1	elongation					1431:1440	active or late elongation	1416:1440	active or late elongation	1416:1440	The expression of glycosyltransferases responsible for mixed-linkage glucan and glucuronoarabinoxylan synthesis peaks at active or late elongation.					
32616810	2	19	theme	unique	250:255	arg1	composition					257:267	The unique composition	246:267	The unique composition of type II primary cell wall characteristic of grasses	246:322	The unique composition of type II primary cell wall characteristic of grasses suggests that they employ specific mechanisms for cell enlargement.					
32616810	2	20	theme	cell	374:377	arg1	enlargement					379:389	cell enlargement	374:389	cell enlargement	374:389	The unique composition of type II primary cell wall characteristic of grasses suggests that they employ specific mechanisms for cell enlargement.					
32616810	0	21	theme	primary	93:99	arg1	walls					106:110	primary cell walls	93:110	primary cell walls	93:110	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	0	22	from	combinations	37:48	arg1	type					74:77	type I	74:79	type I	74:79	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	0	22	from	combinations	37:48	arg1	type					85:88	type II	85:91	type II	85:91	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	9	23	theme	glycosyltransferases	1313:1332	arg1	expression					1299:1308	The expression	1295:1308	The expression of glycosyltransferases responsible for mixed-linkage glucan and glucuronoarabinoxylan synthesis	1295:1405	The expression of glycosyltransferases responsible for mixed-linkage glucan and glucuronoarabinoxylan synthesis peaks at active or late elongation.					
32616810	4	24	theme	growth	794:799	arg1	cessation					770:778	cessation	770:778	cessation	770:778	Specific patterns of cell wall formation differentiate the initiation, realization and cessation of elongation growth.					
32616810	4	24	theme	growth	794:799	arg1	realization					754:764	realization	754:764	realization	754:764	Specific patterns of cell wall formation differentiate the initiation, realization and cessation of elongation growth.					
32616810	4	24	theme	growth	794:799	arg1	initiation					742:751	initiation	742:751	initiation	742:751	Specific patterns of cell wall formation differentiate the initiation, realization and cessation of elongation growth.					
32616810	9	25	theme	responsible	1334:1344	arg1	glycosyltransferases					1313:1332	glycosyltransferases	1313:1332	glycosyltransferases responsible for mixed-linkage glucan and glucuronoarabinoxylan synthesis	1313:1405	The expression of glycosyltransferases responsible for mixed-linkage glucan and glucuronoarabinoxylan synthesis peaks at active or late elongation.					
32616810	3	26	theme	numerous	498:505	arg1	glycosyltransferases					507:526	numerous glycosyltransferases	498:526	numerous glycosyltransferases	498:526	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	2	27	theme	cell	288:291	arg1	wall					293:296	type II primary cell wall	272:296	type II primary cell wall characteristic of grasses	272:322	The unique composition of type II primary cell wall characteristic of grasses suggests that they employ specific mechanisms for cell enlargement.					
32616810	4	28	theme	Specific	683:690	arg1	patterns					692:699	Specific patterns	683:699	Specific patterns of cell wall formation	683:722	Specific patterns of cell wall formation differentiate the initiation, realization and cessation of elongation growth.					
32616810	0	29	theme	Elongating	0:9	arg1	maize					11:15	Elongating maize	0:15	Elongating maize	0:15	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	9	30	theme	glucuronoarabinoxylan	1375:1395	arg1	synthesis					1397:1405	mixed-linkage glucan and glucuronoarabinoxylan synthesis	1350:1405	synthesis	1397:1405	The expression of glycosyltransferases responsible for mixed-linkage glucan and glucuronoarabinoxylan synthesis peaks at active or late elongation.					
32616810	2	31	theme	primary	280:286	arg1	wall					293:296	type II primary cell wall	272:296	type II primary cell wall characteristic of grasses	272:322	The unique composition of type II primary cell wall characteristic of grasses suggests that they employ specific mechanisms for cell enlargement.					
32616810	1	32	theme	cell	172:175	arg1	mechanics					182:190	the cell wall mechanics	168:190	the cell wall mechanics	168:190	The dynamics of cell wall polysaccharides may modulate the cell wall mechanics and thus control the expansion growth of plant cells.					
32616810	5	33	theme	elongation	835:844	arg1	zone					846:849	early elongation zone	829:849	early elongation zone	829:849	Cell walls of meristem and early elongation zone represent a mixture of type I and type II specific polysaccharides.					
32616810	5	34	theme	early	829:833	arg1	zone					846:849	early elongation zone	829:849	early elongation zone	829:849	Cell walls of meristem and early elongation zone represent a mixture of type I and type II specific polysaccharides.					
32616810	0	35	dep	type	74:77	arg1	walls					106:110	primary cell walls	93:110	primary cell walls	93:110	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	3	36	from	changes	595:601	arg1	polysaccharides					616:630	cell wall polysaccharides	606:630	cell wall polysaccharides	606:630	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	1	37	theme	wall	177:180	arg1	mechanics					182:190	the cell wall mechanics	168:190	the cell wall mechanics	168:190	The dynamics of cell wall polysaccharides may modulate the cell wall mechanics and thus control the expansion growth of plant cells.					
32616810	3	38	from	transcriptomes	413:426	arg1	zones					436:440	five zones	431:440	five zones along maize root	431:457	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	6	39	theme	mixed-linkage	998:1010	arg1	glucans					1012:1018	mixed-linkage glucans	998:1018	mixed-linkage glucans	998:1018	Xyloglucans and homogalacturonans are synthesized there actively together with mixed-linkage glucans and glucuronoarabinoxylans.					
32616810	3	40	theme	cell	654:657	arg1	development					659:669	cell development	654:669	cell development	654:669	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	2	41	theme	type	272:275	arg1	wall					293:296	type II primary cell wall	272:296	type II primary cell wall characteristic of grasses	272:322	The unique composition of type II primary cell wall characteristic of grasses suggests that they employ specific mechanisms for cell enlargement.					
32616810	5	42	theme	Cell	802:805	arg1	walls					807:811	Cell walls	802:811	Cell walls of meristem and early elongation zone	802:849	Cell walls of meristem and early elongation zone represent a mixture of type I and type II specific polysaccharides.					
32616810	4	43	theme	cell	704:707	arg1	formation					714:722	cell wall formation	704:722	cell wall formation	704:722	Specific patterns of cell wall formation differentiate the initiation, realization and cessation of elongation growth.					
32616810	3	44	theme	development	659:669	arg1	stages					644:649	critical stages	635:649	critical stages of cell development	635:669	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	2	45	theme	specific	350:357	arg1	mechanisms					359:368	specific mechanisms	350:368	specific mechanisms for cell enlargement	350:389	The unique composition of type II primary cell wall characteristic of grasses suggests that they employ specific mechanisms for cell enlargement.					
32616810	0	46	theme	zone-specific	23:35	arg1	combinations					37:48	zone-specific combinations	23:48	zone-specific combinations of polysaccharides from type I and type II primary cell walls	23:110	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	7	47	theme	cell	1142:1145	arg1	walls					1147:1151	cell walls	1142:1151	cell walls	1142:1151	Rhamnogalacturonans-I with the side-chains of branched 1,4-galactan and arabinan persisted in cell walls throughout the development.					
32616810	0	48	from	type	74:77	arg1	combinations					37:48	zone-specific combinations	23:48	zone-specific combinations of polysaccharides from type I and type II primary cell walls	23:110	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	0	48	from	type	74:77	arg1	polysaccharides					53:67	polysaccharides	53:67	polysaccharides from type I and type II primary cell walls	53:110	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	3	49	theme	cell	606:609	arg1	polysaccharides					616:630	cell wall polysaccharides	606:630	cell wall polysaccharides	606:630	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	8	50	theme	wall	1237:1240	arg1	constituents					1242:1253	the type I primary cell wall constituents	1213:1253	the type I primary cell wall constituents	1213:1253	Thus, the machinery to generate the type I primary cell wall constituents is completely established and operates.					
32616810	0	51	theme	polysaccharides	53:67	arg1	combinations					37:48	zone-specific combinations	23:48	zone-specific combinations of polysaccharides from type I and type II primary cell walls	23:110	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	7	52	theme	arabinan	1120:1127	arg1	side-chains					1079:1089	the side-chains	1075:1089	the side-chains of branched 1,4-galactan and arabinan	1075:1127	Rhamnogalacturonans-I with the side-chains of branched 1,4-galactan and arabinan persisted in cell walls throughout the development.					
32616810	5	53	dep	type	874:877	arg1	polysaccharides					902:916	specific polysaccharides	893:916	specific polysaccharides	893:916	Cell walls of meristem and early elongation zone represent a mixture of type I and type II specific polysaccharides.					
32616810	7	54	theme	branched	1094:1101	arg1	1,4-galactan					1103:1114	branched 1,4-galactan	1094:1114	branched 1,4-galactan	1094:1114	Rhamnogalacturonans-I with the side-chains of branched 1,4-galactan and arabinan persisted in cell walls throughout the development.					
32616810	2	55	theme	grasses	316:322	arg1	characteristic					298:311	characteristic	298:311	characteristic	298:311	The unique composition of type II primary cell wall characteristic of grasses suggests that they employ specific mechanisms for cell enlargement.					
32616810	5	56	theme	meristem	816:823	arg1	walls					807:811	Cell walls	802:811	Cell walls of meristem and early elongation zone	802:849	Cell walls of meristem and early elongation zone represent a mixture of type I and type II specific polysaccharides.					
32616810	9	57	theme	mixed-linkage	1350:1362	arg1	glucan					1364:1369	mixed-linkage glucan and glucuronoarabinoxylan synthesis	1350:1405	glucan	1364:1369	The expression of glycosyltransferases responsible for mixed-linkage glucan and glucuronoarabinoxylan synthesis peaks at active or late elongation.					
32616810	1	58	dep	modulate	159:166	arg1	control					201:207	control	201:207	control the expansion growth of plant cells	201:243	The dynamics of cell wall polysaccharides may modulate the cell wall mechanics and thus control the expansion growth of plant cells.					
32616810	3	59	theme	genes	488:492	arg1	expression					474:483	the expression	470:483	the expression of genes for numerous glycosyltransferases	470:526	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	4	60	theme	formation	714:722	arg1	patterns					692:699	Specific patterns	683:699	Specific patterns of cell wall formation	683:722	Specific patterns of cell wall formation differentiate the initiation, realization and cessation of elongation growth.					
32616810	7	61	theme	1,4-galactan	1103:1114	arg1	side-chains					1079:1089	the side-chains	1075:1089	the side-chains of branched 1,4-galactan and arabinan	1075:1127	Rhamnogalacturonans-I with the side-chains of branched 1,4-galactan and arabinan persisted in cell walls throughout the development.					
32616810	5	62	theme	type	885:888	arg1	mixture					863:869	a mixture	861:869	a mixture of type I and type II specific polysaccharides	861:916	Cell walls of meristem and early elongation zone represent a mixture of type I and type II specific polysaccharides.					
32616810	3	63	dep	transcriptomes	413:426	arg1	performed					532:540	performed	532:540	performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae	532:680	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	3	63	dep	transcriptomes	413:426	arg1	clustered					460:468	clustered	460:468	clustered the expression of genes for numerous glycosyltransferases	460:526	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	2	64	theme	characteristic	298:311	arg1	wall					293:296	type II primary cell wall	272:296	type II primary cell wall characteristic of grasses	272:322	The unique composition of type II primary cell wall characteristic of grasses suggests that they employ specific mechanisms for cell enlargement.					
32616810	3	65	theme	immunohistochemical	552:570	arg1	analysis					572:579	extensive immunohistochemical analysis	542:579	extensive immunohistochemical analysis	542:579	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	3	66	theme	critical	635:642	arg1	stages					644:649	critical stages	635:649	critical stages of cell development	635:669	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	10	67	theme	pieces	1485:1490	arg1	number					1468:1473	the number	1464:1473	the number of jigsaw pieces which should be put together to solve the puzzle of grass cell growth	1464:1560	These findings widen the number of jigsaw pieces which should be put together to solve the puzzle of grass cell growth.					
32616810	2	68	theme	wall	293:296	arg1	composition					257:267	The unique composition	246:267	The unique composition of type II primary cell wall characteristic of grasses	246:322	The unique composition of type II primary cell wall characteristic of grasses suggests that they employ specific mechanisms for cell enlargement.					
32616810	10	69	theme	growth	1555:1560	arg1	puzzle					1534:1539	the puzzle	1530:1539	the puzzle of grass cell growth	1530:1560	These findings widen the number of jigsaw pieces which should be put together to solve the puzzle of grass cell growth.					
32616810	0	70	from	type	85:88	arg1	combinations					37:48	zone-specific combinations	23:48	zone-specific combinations of polysaccharides from type I and type II primary cell walls	23:110	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	0	70	from	type	85:88	arg1	polysaccharides					53:67	polysaccharides	53:67	polysaccharides from type I and type II primary cell walls	53:110	Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.					
32616810	3	71	theme	wall	611:614	arg1	polysaccharides					616:630	cell wall polysaccharides	606:630	cell wall polysaccharides	606:630	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	3	72	theme	maize	448:452	arg1	root					454:457	maize root	448:457	maize root	448:457	We characterized the transcriptomes in five zones along maize root, clustered the expression of genes for numerous glycosyltransferases and performed extensive immunohistochemical analysis to relate the changes in cell wall polysaccharides to critical stages of cell development in Poaceae.					
32616810	5	73	theme	specific	893:900	arg1	polysaccharides					902:916	specific polysaccharides	893:916	specific polysaccharides	893:916	Cell walls of meristem and early elongation zone represent a mixture of type I and type II specific polysaccharides.					
32616810	1	74	theme	expansion	213:221	arg1	growth					223:228	the expansion growth	209:228	the expansion growth of plant cells	209:243	The dynamics of cell wall polysaccharides may modulate the cell wall mechanics and thus control the expansion growth of plant cells.					
32616810	5	75	theme	zone	846:849	arg1	walls					807:811	Cell walls	802:811	Cell walls of meristem and early elongation zone	802:849	Cell walls of meristem and early elongation zone represent a mixture of type I and type II specific polysaccharides.					
32616810	1	76	theme	cell	129:132	arg1	polysaccharides					139:153	cell wall polysaccharides	129:153	cell wall polysaccharides	129:153	The dynamics of cell wall polysaccharides may modulate the cell wall mechanics and thus control the expansion growth of plant cells.					
33865380	12	0	theme	alginate	2422:2429	arg1	microspheres					2431:2442	freeze-dried alginate microspheres	2409:2442	freeze-dried alginate microspheres	2409:2442	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	2	1	theme	essential	364:372	arg1	genes					374:378	the essential genes	360:378	the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis	360:512	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	8	2	theme	in	1619:1620	arg1	functionality					1627:1639	in vivo functionality	1619:1639	in vivo functionality	1619:1639	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	8	3	dep	survived	1424:1431	arg1	showed					1598:1603	showed	1598:1603	showed potential for in vivo functionality	1598:1639	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	7	4	theme	fermentum	1398:1406	arg1	D12					1408:1410	L. fermentum D12	1395:1410	L. fermentum D12	1395:1410	Since recent reports showed that polysaccharides facilitate the probiotic-host interactions, we at first sought to evaluate the functional potential of L. fermentum D12.					
33865380	3	5	theme	precursor	665:673	arg1	galT					691:694	galT	691:694	galT	691:694	These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU.					
33865380	3	5	theme	precursor	665:673	arg1	galE					685:688	galE	685:688	galE	685:688	These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU.					
33865380	3	5	theme	precursor	665:673	arg1	galU					700:703	galU	700:703	galU	700:703	These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU.					
33865380	3	5	theme	precursor	665:673	arg1	molecules					675:683	the precursor molecules galE, galT and galU	661:703	the precursor molecules galE, galT and galU	661:703	These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU.					
33865380	6	6	theme	branched	1160:1167	arg1	heteropolysaccharide					1169:1188	a branched heteropolysaccharide	1158:1188	a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf	1158:1226	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	1	7	theme	putative	284:291	arg1	operon					297:302	a putative eps operon	282:302	a putative eps operon	282:302	Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon.					
33865380	12	8	theme	fermentum	2392:2400	arg1	D12					2402:2404	L. fermentum D12	2389:2404	L. fermentum D12	2389:2404	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	3	9	theme	EPS	553:555	arg1	biosynthesis					557:568	EPS biosynthesis	553:568	EPS biosynthesis	553:568	These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU.					
33865380	1	10	theme	fermentum	199:207	arg1	D12					209:211	Lactobacillus (Limosilactobacillus) fermentum D12	163:211	Lactobacillus (Limosilactobacillus) fermentum D12	163:211	Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon.					
33865380	1	10	theme	fermentum	199:207	arg1	exopolysaccharide					219:235	an exopolysaccharide	216:235	an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon	216:302	Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon.					
33865380	7	11	theme	functional	1371:1380	arg1	potential					1382:1390	the functional potential	1367:1390	the functional potential of L. fermentum D12	1367:1410	Since recent reports showed that polysaccharides facilitate the probiotic-host interactions, we at first sought to evaluate the functional potential of L. fermentum D12.					
33865380	10	12	theme	simulated	2000:2008	arg1	conditions					2014:2023	simulated GIT conditions	2000:2023	simulated GIT conditions	2000:2023	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	6	13	theme	different	1142:1150	arg1	EPSs					1152:1155	at least two different EPSs	1129:1155	at least two different EPSs	1129:1155	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	2	14	theme	D12	330:332	arg1	analysis					318:325	Whole-genome analysis	305:325	Whole-genome analysis of D12	305:332	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	4	15	dep	biosynthesis	715:726	arg1	mechanism					739:747	mechanism	739:747	mechanism	739:747	Both the biosynthesis and export mechanism of EPS were proposed based on functional annotation.					
33865380	4	15	dep	biosynthesis	715:726	arg1	the					711:713	the	711:713	the	711:713	Both the biosynthesis and export mechanism of EPS were proposed based on functional annotation.					
33865380	9	16	theme	0.2	1872:1874	arg1	concentrations					1854:1867	concentrations	1854:1867	concentrations of 0.2, 0.5, and 1.0 mg/mL	1854:1894	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	5	17	dep	200 mg/L	893:900	arg1	up					887:888	up	887:888	up	887:888	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	5	18	theme	electron	974:981	arg1	SEM					995:997	SEM	995:997	SEM	995:997	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	5	18	theme	electron	974:981	arg1	microscopy					983:992	scanning electron microscopy	965:992	scanning electron microscopy (SEM)	965:998	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	9	19	theme	EPS	1646:1648	arg1	extract					1656:1662	The EPS crude extract	1642:1662	The EPS crude extract	1642:1662	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	9	20	theme	0.5	1877:1879	arg1	concentrations					1854:1867	concentrations	1854:1867	concentrations of 0.2, 0.5, and 1.0 mg/mL	1854:1894	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	5	21	theme	porous	930:935	arg1	structure					937:945	structure	937:945	structure	937:945	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	5	22	theme	2	845:845	arg1	%					846:846	%	846:846	%	846:846	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	12	23	from	viability	2376:2384	arg1	microspheres					2431:2442	freeze-dried alginate microspheres	2409:2442	freeze-dried alginate microspheres	2409:2442	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	5	24	theme	w/v	848:850	arg1	glucose					852:858	an additional 2% w/v glucose	831:858	an additional 2% w/v glucose	831:858	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	7	25	theme	recent	1249:1254	arg1	reports					1256:1262	recent reports	1249:1262	recent reports	1249:1262	Since recent reports showed that polysaccharides facilitate the probiotic-host interactions, we at first sought to evaluate the functional potential of L. fermentum D12.					
33865380	10	26	theme	bacterial	1914:1922	arg1	count					1924:1928	the viable bacterial count	1903:1928	the viable bacterial count of free D12 cells	1903:1946	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	10	26	theme	bacterial	1914:1922	arg1	lower					1972:1976	lower	1972:1976	lower	1972:1976	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	12	27	dep	substrates	2329:2338	arg1	substrates					2329:2338	the prebiotic substrates mannitol and lactulose	2315:2361	the prebiotic substrates mannitol and lactulose	2315:2361	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	12	27	dep	substrates	2329:2338	arg1	lactulose					2353:2361	lactulose	2353:2361	lactulose	2353:2361	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	12	27	dep	substrates	2329:2338	arg1	mannitol					2340:2347	mannitol	2340:2347	mannitol	2340:2347	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	1	28	theme	Lactobacillus	163:175	arg1	D12					209:211	Lactobacillus (Limosilactobacillus) fermentum D12	163:211	Lactobacillus (Limosilactobacillus) fermentum D12	163:211	Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon.					
33865380	1	28	theme	Lactobacillus	163:175	arg1	exopolysaccharide					219:235	an exopolysaccharide	216:235	an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon	216:302	Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon.					
33865380	2	29	theme	synthesis	504:512	arg1	elongation					431:440	elongation	431:440	elongation	431:440	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	2	29	theme	synthesis	504:512	arg1	activation					396:405	activation	396:405	activation of precursor molecules	396:428	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	2	29	theme	synthesis	504:512	arg1	regulation					486:495	regulation	486:495	regulation of EPS synthesis	486:512	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	2	29	theme	synthesis	504:512	arg1	export					446:451	export	446:451	export	446:451	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	8	30	theme	Strain	1413:1418	arg1	D12					1420:1422	Strain D12	1413:1422	Strain D12	1413:1422	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	1	31	theme	Limosilactobacillus	178:196	arg1	D12					209:211	Lactobacillus (Limosilactobacillus) fermentum D12	163:211	Lactobacillus (Limosilactobacillus) fermentum D12	163:211	Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon.					
33865380	1	31	theme	Limosilactobacillus	178:196	arg1	exopolysaccharide					219:235	an exopolysaccharide	216:235	an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon	216:302	Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon.					
33865380	11	32	theme	D12	2142:2144	arg1	cells					2146:2150	D12 cells	2142:2150	D12 cells	2142:2150	Microspheres of D12 cells, which were previously analyzed at SEM, significantly influenced their survival during freeze-drying and in simulated GIT conditions.					
33865380	10	33	theme	D12	1938:1940	arg1	cells					1942:1946	free D12 cells	1933:1946	free D12 cells	1933:1946	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	8	34	theme	gastrointestinal	1443:1458	arg1	tract					1460:1464	simulated gastrointestinal tract	1433:1464	simulated gastrointestinal tract (GIT) conditions	1433:1481	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	8	34	theme	gastrointestinal	1443:1458	arg1	GIT					1467:1469	GIT	1467:1469	GIT	1467:1469	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	11	35	theme	GIT	2270:2272	arg1	conditions					2274:2283	simulated GIT conditions	2260:2283	simulated GIT conditions	2260:2283	Microspheres of D12 cells, which were previously analyzed at SEM, significantly influenced their survival during freeze-drying and in simulated GIT conditions.					
33865380	2	36	theme	molecules	420:428	arg1	elongation					431:440	elongation	431:440	elongation	431:440	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	2	36	theme	molecules	420:428	arg1	activation					396:405	activation	396:405	activation of precursor molecules	396:428	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	2	36	theme	molecules	420:428	arg1	regulation					486:495	regulation	486:495	regulation of EPS synthesis	486:512	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	2	36	theme	molecules	420:428	arg1	export					446:451	export	446:451	export	446:451	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	6	37	contain	containing	1190:1199	arg2	t-Glcp					1201:1206	t-Glcp	1201:1206	t-Glcp	1201:1206	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	6	37	contain	containing	1190:1199	arg1	heteropolysaccharide					1169:1188	a branched heteropolysaccharide	1158:1188	a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf	1158:1226	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	6	37	contain	containing	1190:1199	arg2	Galf					1223:1226	2,6-linked Galf	1212:1226	2,6-linked Galf	1212:1226	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	2	38	theme	chain	475:479	arg1	elongation					431:440	elongation	431:440	elongation	431:440	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	2	38	theme	chain	475:479	arg1	activation					396:405	activation	396:405	activation of precursor molecules	396:428	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	2	38	theme	chain	475:479	arg1	regulation					486:495	regulation	486:495	regulation of EPS synthesis	486:512	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	2	38	theme	chain	475:479	arg1	export					446:451	export	446:451	export	446:451	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	0	39	theme	Lyophilized	0:10	arg1	microspheres					27:38	Lyophilized alginate-based microspheres	0:38	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer,	0:110	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.					
33865380	1	40	theme	eps	293:295	arg1	operon					297:302	a putative eps operon	282:302	a putative eps operon	282:302	Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon.					
33865380	5	41	theme	fermentum	864:872	arg1	D12					874:876	L. fermentum D12	861:876	L. fermentum D12	861:876	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	9	42	theme	extracellular	1755:1767	arg1	matrix					1769:1774	extracellular matrix	1755:1774	extracellular matrix (ECM) proteins	1755:1789	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	9	42	theme	extracellular	1755:1767	arg1	ECM					1777:1779	ECM	1777:1779	ECM	1777:1779	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	4	43	theme	EPS	752:754	arg1	export					732:737	export	732:737	export	732:737	Both the biosynthesis and export mechanism of EPS were proposed based on functional annotation.					
33865380	4	43	theme	EPS	752:754	arg1	biosynthesis					715:726	biosynthesis	715:726	biosynthesis	715:726	Both the biosynthesis and export mechanism of EPS were proposed based on functional annotation.					
33865380	0	44	theme	Lactobacillus	51:63	arg1	producer					102:109	an exopolysaccharides producer	80:109	an exopolysaccharides producer	80:109	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.					
33865380	0	44	theme	Lactobacillus	51:63	arg1	D12					75:77	Lactobacillus fermentum D12	51:77	Lactobacillus fermentum D12	51:77	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.					
33865380	5	45	theme	EPSs	918:921	arg1	mixture					907:913	a mixture	905:913	a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM)	905:998	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	8	46	theme	antibacterial	1494:1506	arg1	activity					1508:1515	antibacterial activity	1494:1515	antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells	1494:1574	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	0	47	dep	Lactobacillus	51:63	arg1	fermentum					65:73	fermentum	65:73	fermentum	65:73	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.					
33865380	12	48	theme	prebiotic	2319:2327	arg1	substrates					2329:2338	the prebiotic substrates mannitol and lactulose	2315:2361	the prebiotic substrates mannitol and lactulose	2315:2361	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	12	48	theme	prebiotic	2319:2327	arg1	lactulose					2353:2361	lactulose	2353:2361	lactulose	2353:2361	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	12	48	theme	prebiotic	2319:2327	arg1	mannitol					2340:2347	mannitol	2340:2347	mannitol	2340:2347	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	12	49	from	4 °C	2469:2472	arg1	storage					2458:2464	1-year storage	2451:2464	1-year storage at 4 °C	2451:2472	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	6	50	theme	Structural	1001:1010	arg1	information					1012:1022	Structural information	1001:1022	Structural information	1001:1022	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	10	51	theme	logarithmic	1954:1964	arg1	units					1966:1970	3 logarithmic units	1952:1970	3 logarithmic units	1952:1970	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	12	52	theme	freeze-dried	2409:2420	arg1	microspheres					2431:2442	freeze-dried alginate microspheres	2409:2442	freeze-dried alginate microspheres	2409:2442	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	7	53	theme	D12	1408:1410	arg1	potential					1382:1390	the functional potential	1367:1390	the functional potential of L. fermentum D12	1367:1410	Since recent reports showed that polysaccharides facilitate the probiotic-host interactions, we at first sought to evaluate the functional potential of L. fermentum D12.					
33865380	5	54	theme	mixture	907:913	arg1	200 mg/L					893:900	200 mg/L	893:900	200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM)	893:998	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	6	55	link	2,6-linked	1212:1221	arg1	Galf					1223:1226	2,6-linked Galf	1212:1226	2,6-linked Galf	1212:1226	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	8	56	theme	adhered	1552:1558	arg1	bacteria					1542:1549	enteropathogenic bacteria	1525:1549	enteropathogenic bacteria	1525:1549	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	9	57	theme	strain	1690:1695	arg1	capacity					1697:1704	D12 strain capacity	1686:1704	D12 strain capacity	1686:1704	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	7	58	theme	L.	1395:1396	arg1	D12					1408:1410	L. fermentum D12	1395:1410	L. fermentum D12	1395:1410	Since recent reports showed that polysaccharides facilitate the probiotic-host interactions, we at first sought to evaluate the functional potential of L. fermentum D12.					
33865380	12	59	theme	1-year	2451:2456	arg1	storage					2458:2464	1-year storage	2451:2464	1-year storage at 4 °C	2451:2472	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	8	60	theme	Caco-2	1563:1568	arg1	cells					1570:1574	Caco-2 cells	1563:1574	Caco-2 cells	1563:1574	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	2	61	theme	Whole-genome	305:316	arg1	analysis					318:325	Whole-genome analysis	305:325	Whole-genome analysis of D12	305:332	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	6	62	theme	linkage	1085:1091	arg1	analyses					1093:1100	linkage analyses	1085:1100	linkage analyses	1085:1100	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	9	63	theme	mucin	1824:1828	arg1	adherence					1830:1838	mucin adherence	1824:1838	mucin adherence	1824:1838	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	3	64	theme	molecules	675:683	arg1	activation					647:656	the activation	643:656	the activation of the precursor molecules galE, galT and galU	643:703	These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU.					
33865380	10	65	theme	GIT	2010:2012	arg1	conditions					2014:2023	simulated GIT conditions	2000:2023	simulated GIT conditions	2000:2023	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	12	66	theme	substrates	2329:2338	arg1	addition					2303:2310	the addition	2299:2310	the addition of the prebiotic substrates mannitol and lactulose	2299:2361	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	6	67	theme	EPSs	1152:1155	arg1	heteropolysaccharide					1169:1188	a branched heteropolysaccharide	1158:1188	a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf	1158:1226	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	6	67	theme	EPSs	1152:1155	arg1	presence					1117:1124	the presence	1113:1124	the presence of at least two different EPSs	1113:1155	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	6	67	theme	EPSs	1152:1155	arg1	glycogen					1233:1240	glycogen	1233:1240	glycogen	1233:1240	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	0	68	contain	containing	40:49	arg1	microspheres					27:38	Lyophilized alginate-based microspheres	0:38	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer,	0:110	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.					
33865380	0	68	contain	containing	40:49	arg2	D12					75:77	Lactobacillus fermentum D12	51:77	Lactobacillus fermentum D12	51:77	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.					
33865380	0	68	contain	containing	40:49	arg2	producer					102:109	an exopolysaccharides producer	80:109	an exopolysaccharides producer	80:109	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.					
33865380	4	69	theme	functional	779:788	arg1	annotation					790:799	functional annotation	779:799	functional annotation	779:799	Both the biosynthesis and export mechanism of EPS were proposed based on functional annotation.					
33865380	6	70	theme	1HNMR	1036:1040	arg1	spectroscopy					1042:1053	1HNMR spectroscopy	1036:1053	1HNMR spectroscopy together with composition and linkage analyses	1036:1100	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	10	71	theme	initial	2034:2040	arg1	count					2042:2046	the initial count	2030:2046	the initial count	2030:2046	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	9	72	dep	influenced	1675:1684	arg1	adhere					1745:1750	adhere	1745:1750	to adhere to extracellular matrix (ECM) proteins	1742:1789	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	9	72	dep	influenced	1675:1684	arg1	survive					1709:1715	survive	1709:1715	to survive during freeze-drying	1706:1736	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	5	73	theme	scanning	965:972	arg1	SEM					995:997	SEM	995:997	SEM	995:997	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	5	73	theme	scanning	965:972	arg1	microscopy					983:992	scanning electron microscopy	965:992	scanning electron microscopy (SEM)	965:998	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	7	74	theme	probiotic-host	1307:1320	arg1	interactions					1322:1333	the probiotic-host interactions	1303:1333	the probiotic-host interactions	1303:1333	Since recent reports showed that polysaccharides facilitate the probiotic-host interactions, we at first sought to evaluate the functional potential of L. fermentum D12.					
33865380	8	75	dep	in	1619:1620	arg1	vivo					1622:1625	vivo	1622:1625	vivo	1622:1625	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	5	76	theme	additional	834:843	arg1	glucose					852:858	an additional 2% w/v glucose	831:858	an additional 2% w/v glucose	831:858	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	9	77	theme	crude	1650:1654	arg1	extract					1656:1662	The EPS crude extract	1642:1662	The EPS crude extract	1642:1662	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	10	78	theme	bacterial	2053:2061	arg1	cells					2063:2067	the bacterial cells	2049:2067	the bacterial cells	2049:2067	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	12	79	theme	D12	2402:2404	arg1	viability					2376:2384	the viability	2372:2384	the viability of L. fermentum D12 in freeze-dried alginate microspheres	2372:2442	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	6	80	theme	2,6-linked	1212:1221	arg1	Galf					1223:1226	2,6-linked Galf	1212:1226	2,6-linked Galf	1212:1226	Structural information obtained by 1HNMR spectroscopy together with composition and linkage analyses, suggested the presence of at least two different EPSs, a branched heteropolysaccharide containing t-Glcp and 2,6-linked Galf, and glycogen.					
33865380	5	81	theme	%	846:846	arg1	glucose					852:858	an additional 2% w/v glucose	831:858	an additional 2% w/v glucose	831:858	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	9	82	theme	1.0 mg/mL	1886:1894	arg1	concentrations					1854:1867	concentrations	1854:1867	concentrations of 0.2, 0.5, and 1.0 mg/mL	1854:1894	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	0	83	theme	exopolysaccharides	83:100	arg1	producer					102:109	an exopolysaccharides producer	80:109	an exopolysaccharides producer	80:109	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.					
33865380	0	83	theme	exopolysaccharides	83:100	arg1	D12					75:77	Lactobacillus fermentum D12	51:77	Lactobacillus fermentum D12	51:77	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.					
33865380	1	84	contain	contains	273:280	arg2	operon					297:302	a putative eps operon	282:302	a putative eps operon	282:302	Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon.					
33865380	1	84	contain	contains	273:280	arg1	genome					266:271	genome	266:271	genome	266:271	Lactobacillus (Limosilactobacillus) fermentum D12 is an exopolysaccharide (EPS) producing strain whose genome contains a putative eps operon.					
33865380	5	85	theme	MRS	816:818	arg1	broth					820:824	MRS broth	816:824	MRS broth with an additional 2% w/v glucose	816:858	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	9	86	theme	D12	1686:1688	arg1	capacity					1697:1704	D12 strain capacity	1686:1704	D12 strain capacity	1686:1704	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	10	87	theme	viable	1907:1912	arg1	count					1924:1928	the viable bacterial count	1903:1928	the viable bacterial count of free D12 cells	1903:1946	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	10	87	theme	viable	1907:1912	arg1	lower					1972:1976	lower	1972:1976	lower	1972:1976	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	2	88	theme	EPS	500:502	arg1	synthesis					504:512	EPS synthesis	500:512	EPS synthesis	500:512	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	8	89	theme	simulated	1433:1441	arg1	tract					1460:1464	simulated gastrointestinal tract	1433:1464	simulated gastrointestinal tract (GIT) conditions	1433:1481	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	8	89	theme	simulated	1433:1441	arg1	GIT					1467:1469	GIT	1467:1469	GIT	1467:1469	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	10	90	theme	cells	1942:1946	arg1	count					1924:1928	the viable bacterial count	1903:1928	the viable bacterial count of free D12 cells	1903:1946	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	10	90	theme	cells	1942:1946	arg1	lower					1972:1976	lower	1972:1976	lower	1972:1976	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	11	91	theme	simulated	2260:2268	arg1	conditions					2274:2283	simulated GIT conditions	2260:2283	simulated GIT conditions	2260:2283	Microspheres of D12 cells, which were previously analyzed at SEM, significantly influenced their survival during freeze-drying and in simulated GIT conditions.					
33865380	11	92	theme	cells	2146:2150	arg1	Microspheres					2126:2137	Microspheres	2126:2137	Microspheres	2126:2137	Microspheres of D12 cells, which were previously analyzed at SEM, significantly influenced their survival during freeze-drying and in simulated GIT conditions.					
33865380	8	93	theme	tract	1460:1464	arg1	conditions					1472:1481	simulated gastrointestinal tract (GIT) conditions	1433:1481	simulated gastrointestinal tract (GIT) conditions	1433:1481	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	5	94	with	broth	820:824	arg1	glucose					852:858	an additional 2% w/v glucose	831:858	an additional 2% w/v glucose	831:858	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	10	95	theme	viability	2103:2111	arg1	improvement					2113:2123	viability improvement	2103:2123	viability improvement	2103:2123	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	12	96	theme	L.	2389:2390	arg1	D12					2402:2404	L. fermentum D12	2389:2404	L. fermentum D12	2389:2404	Furthermore, the addition of the prebiotic substrates mannitol and lactulose improved the viability of L. fermentum D12 in freeze-dried alginate microspheres during 1-year storage at 4 °C compared to the control.					
33865380	10	97	theme	free	1933:1936	arg1	cells					1942:1946	free D12 cells	1933:1946	free D12 cells	1933:1946	Since the viable bacterial count of free D12 cells was 3 logarithmic units lower after the exposure to simulated GIT conditions than the initial count, the bacterial cells had been loaded into alginate for viability improvement.					
33865380	2	98	theme	precursor	410:418	arg1	molecules					420:428	precursor molecules	410:428	precursor molecules	410:428	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	0	99	theme	alginate-based	12:25	arg1	microspheres					27:38	Lyophilized alginate-based microspheres	0:38	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer,	0:110	Lyophilized alginate-based microspheres containing Lactobacillus fermentum D12, an exopolysaccharides producer, contribute to the strain's functionality in vitro.					
33865380	2	100	theme	polysaccharide	460:473	arg1	chain					475:479	the polysaccharide chain	456:479	the polysaccharide chain	456:479	Whole-genome analysis of D12 was performed to disclose the essential genes correlated with activation of precursor molecules, elongation and export of the polysaccharide chain, and regulation of EPS synthesis.					
33865380	5	101	theme	L.	861:862	arg1	D12					874:876	L. fermentum D12	861:876	L. fermentum D12	861:876	When grown on MRS broth with an additional 2% w/v glucose, L. fermentum D12 secreted up to 200 mg/L of a mixture of EPSs, whose porous structure was visualized by scanning electron microscopy (SEM).					
33865380	9	102	theme	matrix	1769:1774	arg1	proteins					1782:1789	extracellular matrix (ECM) proteins	1755:1789	extracellular matrix (ECM) proteins	1755:1789	The EPS crude extract positively influenced D12 strain capacity to survive during freeze-drying and to adhere to extracellular matrix (ECM) proteins but did not interfere Caco-2 and mucin adherence when added at concentrations of 0.2, 0.5, and 1.0 mg/mL.					
33865380	8	103	theme	enteropathogenic	1525:1540	arg1	bacteria					1542:1549	enteropathogenic bacteria	1525:1549	enteropathogenic bacteria	1525:1549	Strain D12 survived simulated gastrointestinal tract (GIT) conditions, exhibited antibacterial activity against enteropathogenic bacteria, adhered to Caco-2 cells in vitro, and as such showed potential for in vivo functionality.					
33865380	3	104	dep	molecules	675:683	arg1	galT					691:694	galT	691:694	galT	691:694	These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU.					
33865380	3	104	dep	molecules	675:683	arg1	galE					685:688	galE	685:688	galE	685:688	These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU.					
33865380	3	104	dep	molecules	675:683	arg1	galU					700:703	galU	700:703	galU	700:703	These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU.					
33865380	3	104	dep	molecules	675:683	arg1	molecules					675:683	the precursor molecules galE, galT and galU	661:703	the precursor molecules galE, galT and galU	661:703	These included the genes required for EPS biosynthesis such as epsA, B, C, D and E, also gt, wzx, and wzy and those involved in the activation of the precursor molecules galE, galT and galU.					
33142219	14	0	theme	oil	2361:2363	arg1	extraction					2365:2374	oil extraction	2361:2374	oil extraction	2361:2374	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	6	1	theme	bacteria	760:767	arg1	campestris					796:805	Xanthomonas campestris	784:805	Xanthomonas campestris	784:805	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	6	1	theme	bacteria	760:767	arg1	species					769:775	Certain bacteria species	752:775	Certain bacteria species (e.g., Xanthomonas campestris)	752:806	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	11	2	theme	XG	1732:1733	arg1	extent					1722:1727	the extent	1718:1727	the extent of XG and pyruvate (pyruvyl mannose) production	1718:1775	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	13	3	theme	mannose	2031:2037	arg1	content					2039:2045	pyruvate acetyl mannose content	2015:2045	pyruvate acetyl mannose content	2015:2045	In contrast, both pyruvate acetyl mannose content went up in the dialysis-water culture.					
33142219	14	4	theme	practices	2347:2355	arg1	implementation					2317:2330	the implementation	2313:2330	the implementation of sustainable practices for oil extraction	2313:2374	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	10	5	from	excitation	1566:1575	arg1	spectroscopy					1542:1553	Raman spectroscopy	1536:1553	Raman spectroscopy at 1064 nm excitation	1536:1575	XG produced by these cultures was analyzed by Raman spectroscopy at 1064 nm excitation and subjected to principal component analysis (PCA).					
33142219	7	6	from	photo-stimulation	973:989	arg1	use					925:927	the use	921:927	the use of produced water (PW) water in combination photo-stimulation with laser/LED	921:1004	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	8	7	used	used	1088:1091	arg2	spectroscopy					1066:1077	Raman spectroscopy	1060:1077	Raman spectroscopy	1060:1077	Raman spectroscopy has been used for qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation.					
33142219	4	8	theme	microbial	583:591	arg1	MEOR					616:619	MEOR	616:619	MEOR	616:619	In this context, microbial enhanced oil recovery (MEOR) represents an attractive alternative.					
33142219	4	8	theme	microbial	583:591	arg1	recovery					606:613	microbial enhanced oil recovery	583:613	microbial enhanced oil recovery (MEOR)	583:620	In this context, microbial enhanced oil recovery (MEOR) represents an attractive alternative.					
33142219	9	9	from	cultures	1224:1231	arg1	water					1253:1257	distilled water	1243:1257	distilled water	1243:1257	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	9	from	cultures	1224:1231	arg1	water					1280:1284	dialysis-produced water	1262:1284	dialysis-produced water	1262:1284	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	0	10	theme	Raman	172:176	arg1	spectroscopy					178:189	Raman spectroscopy	172:189	Raman spectroscopy	172:189	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	14	11	theme	compounds	2260:2268	arg1	production					2246:2255	bacterial production	2236:2255	bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction	2236:2374	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	9	12	theme	size	1372:1375	arg1	0.040 cm2					1377:1385	spot size 0.040 cm2	1367:1385	spot size 0.040 cm2	1367:1385	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	12	theme	size	1372:1375	arg1	λ = 660 nm					1351:1360	λ = 660 nm	1351:1360	λ = 660 nm	1351:1360	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	4	13	theme	oil	602:604	arg1	MEOR					616:619	MEOR	616:619	MEOR	616:619	In this context, microbial enhanced oil recovery (MEOR) represents an attractive alternative.					
33142219	4	13	theme	oil	602:604	arg1	recovery					606:613	microbial enhanced oil recovery	583:613	microbial enhanced oil recovery (MEOR)	583:620	In this context, microbial enhanced oil recovery (MEOR) represents an attractive alternative.					
33142219	11	14	theme	general	1676:1682	arg1	GLM					1698:1700	GLM	1698:1700	GLM	1698:1700	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	11	14	theme	general	1676:1682	arg1	model					1691:1695	general linear model	1676:1695	general linear model (GLM)	1676:1701	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	7	15	from	use	925:927	arg1	photo-stimulation					973:989	combination photo-stimulation	961:989	combination photo-stimulation with laser/LED	961:1004	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	10	16	theme	principal	1594:1602	arg1	PCA					1624:1626	PCA	1624:1626	PCA	1624:1626	XG produced by these cultures was analyzed by Raman spectroscopy at 1064 nm excitation and subjected to principal component analysis (PCA).					
33142219	10	16	theme	principal	1594:1602	arg1	analysis					1614:1621	principal component analysis	1594:1621	principal component analysis (PCA)	1594:1627	XG produced by these cultures was analyzed by Raman spectroscopy at 1064 nm excitation and subjected to principal component analysis (PCA).					
33142219	14	17	theme	opportunities	2121:2133	arg1	field					2112:2116	a wide field	2105:2116	a wide field of opportunities	2105:2133	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	7	18	theme	combination	961:971	arg1	photo-stimulation					973:989	combination photo-stimulation	961:989	combination photo-stimulation with laser/LED	961:1004	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	7	19	theme	produced	932:939	arg1	PW					948:949	PW	948:949	PW	948:949	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	7	19	theme	produced	932:939	arg1	water					941:945	produced water	932:945	produced water (PW) water in combination photo-stimulation with laser/LED	932:1004	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	8	20	theme	composition	1156:1166	arg1	evaluation					1126:1135	qualitative and quantitative evaluation	1097:1135	qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation	1097:1207	Raman spectroscopy has been used for qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation.					
33142219	6	21	theme	Xanthan	882:888	arg1	XG					895:896	XG	895:896	XG	895:896	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	6	21	theme	Xanthan	882:888	arg1	gum					890:892	the well-known Xanthan gum	867:892	the well-known Xanthan gum (XG)	867:897	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	0	22	theme	dialyzed	129:136	arg1	water					154:158	dialyzed or not produced water	129:158	dialyzed or not produced water	129:158	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	12	23	theme	distilled	1972:1980	arg1	culture					1988:1994	the distilled water culture	1968:1994	the distilled water culture	1968:1994	XG production increased in the distilled water culture.					
33142219	2	24	theme	oil	423:425	arg1	recovery					427:434	oil recovery	423:434	oil recovery	423:434	However, high demand for energy and the decline of the production of oil fields makes oil recovery a challenge.					
33142219	9	25	theme	campestris	1213:1222	arg1	cultures					1224:1231	X. campestris cultures	1210:1231	X. campestris cultures in either distilled water or dialysis-produced water	1210:1284	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	5	26	theme	microorganisms	711:724	arg1	metabolism					697:706	the metabolism	693:706	the metabolism of microorganisms that produce biopolymers	693:749	It employs products derived from the metabolism of microorganisms that produce biopolymers.					
33142219	2	27	theme	fields	410:415	arg1	production					392:401	the production	388:401	the production of oil fields	388:415	However, high demand for energy and the decline of the production of oil fields makes oil recovery a challenge.					
33142219	11	28	theme	exploratory	1645:1655	arg1	analysis					1657:1664	the exploratory analysis	1641:1664	the exploratory analysis	1641:1664	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	0	29	theme	gum	77:79	arg1	composition					54:64	the composition	50:64	the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy	50:189	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	9	30	theme	distilled	1243:1251	arg1	water					1253:1257	distilled water	1243:1257	distilled water	1243:1257	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	31	theme	spot	1444:1447	arg1	λ = 630 nm ± 2 nm					1421:1437	λ = 630 nm ± 2 nm	1421:1437	λ = 630 nm ± 2 nm	1421:1437	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	31	theme	spot	1444:1447	arg1	0.50 cm2					1454:1461	spot size 0.50 cm2	1444:1461	spot size 0.50 cm2	1444:1461	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	14	32	theme	metal-enriched	2149:2162	arg1	cultures					2164:2171	metal-enriched cultures	2149:2171	metal-enriched cultures	2149:2171	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	11	33	theme	mannose	1757:1763	arg1	production					1766:1775	pyruvate (pyruvyl mannose) production	1739:1775	pyruvate (pyruvyl mannose) production	1739:1775	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	14	34	from	use	2142:2144	arg1	combination					2176:2186	combination	2176:2186	combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction	2176:2374	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	9	35	dep	absence	1309:1315	arg1	the					1305:1307	the	1305:1307	the	1305:1307	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	7	36	dep	water	941:945	arg1	water					952:956	water	952:956	produced water (PW) water in combination photo-stimulation with laser/LED	932:1004	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	11	37	dep	analysis	1657:1664	arg1	GLM					1698:1700	GLM	1698:1700	GLM	1698:1700	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	11	37	dep	analysis	1657:1664	arg1	model					1691:1695	general linear model	1676:1695	general linear model (GLM)	1676:1701	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	10	38	theme	1064 nm	1558:1564	arg1	excitation					1566:1575	1064 nm excitation	1558:1575	1064 nm excitation	1558:1575	XG produced by these cultures was analyzed by Raman spectroscopy at 1064 nm excitation and subjected to principal component analysis (PCA).					
33142219	0	39	theme	photo-stimulation	11:27	arg1	Effects					0:6	Effects	0:6	Effects of photo-stimulation with laser or	0:41	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	1	40	theme	important	242:250	arg1	sources					252:258	the most important sources	233:258	the most important sources of energy	233:268	Oil is expected to continue to be one of the most important sources of energy in the world and world's energy matrix for the foreseeable future.					
33142219	11	41	theme	production	1766:1775	arg1	extent					1722:1727	the extent	1718:1727	the extent of XG and pyruvate (pyruvyl mannose) production	1718:1775	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	2	42	theme	high	346:349	arg1	demand					351:356	high demand	346:356	high demand for energy and the decline of the production of oil fields	346:415	However, high demand for energy and the decline of the production of oil fields makes oil recovery a challenge.					
33142219	8	43	theme	XG	1171:1172	arg1	biopolymer					1174:1183	XG biopolymer	1171:1183	XG biopolymer	1171:1183	Raman spectroscopy has been used for qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation.					
33142219	0	44	theme	laser	34:38	arg1	or					40:41	laser or	34:41	laser or	34:41	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	5	45	attach	derived	680:686	arg2	products					671:678	products	671:678	products derived from the metabolism of microorganisms that produce biopolymers	671:749	It employs products derived from the metabolism of microorganisms that produce biopolymers.					
33142219	5	45	attach	derived	680:686	arg1	metabolism					697:706	the metabolism	693:706	the metabolism of microorganisms that produce biopolymers	693:749	It employs products derived from the metabolism of microorganisms that produce biopolymers.					
33142219	0	46	with	Effects	0:6	arg1	or					40:41	laser or	34:41	laser or	34:41	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	6	47	dep	polysaccharides	816:830	arg1	exopolysaccharides					833:850	exopolysaccharides	833:850	exopolysaccharides	833:850	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	6	47	dep	polysaccharides	816:830	arg1	EPS					854:856	EPS	854:856	EPS	854:856	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	11	48	dep	X.	1808:1809	arg1	campestris					1811:1820	X. campestris	1808:1820	X. campestris	1808:1820	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	9	49	theme	irradiation	1338:1348	arg1	absence					1309:1315	absence	1309:1315	absence	1309:1315	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	49	theme	irradiation	1338:1348	arg1	presence					1320:1327	presence	1320:1327	presence	1320:1327	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	10	50	theme	component	1604:1612	arg1	PCA					1624:1626	PCA	1624:1626	PCA	1624:1626	XG produced by these cultures was analyzed by Raman spectroscopy at 1064 nm excitation and subjected to principal component analysis (PCA).					
33142219	10	50	theme	component	1604:1612	arg1	analysis					1614:1621	principal component analysis	1594:1621	principal component analysis (PCA)	1594:1627	XG produced by these cultures was analyzed by Raman spectroscopy at 1064 nm excitation and subjected to principal component analysis (PCA).					
33142219	13	51	theme	acetyl	2024:2029	arg1	content					2039:2045	pyruvate acetyl mannose content	2015:2045	pyruvate acetyl mannose content	2015:2045	In contrast, both pyruvate acetyl mannose content went up in the dialysis-water culture.					
33142219	8	52	theme	Raman	1060:1064	arg1	spectroscopy					1066:1077	Raman spectroscopy	1060:1077	Raman spectroscopy	1060:1077	Raman spectroscopy has been used for qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation.					
33142219	14	53	with	combination	2176:2186	arg1	photo-biomodulation					2193:2211	photo-biomodulation	2193:2211	photo-biomodulation	2193:2211	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	6	54	theme	Certain	752:758	arg1	campestris					796:805	Xanthomonas campestris	784:805	Xanthomonas campestris	784:805	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	6	54	theme	Certain	752:758	arg1	species					769:775	Certain bacteria species	752:775	Certain bacteria species (e.g., Xanthomonas campestris)	752:806	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	14	55	theme	sustainable	2335:2345	arg1	practices					2347:2355	sustainable practices	2335:2355	sustainable practices for oil extraction	2335:2374	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	3	56	theme	Most	449:452	arg1	techniques					454:463	Most techniques	449:463	Most techniques used for the recovery process	449:493	Most techniques used for the recovery process are expensive, non-sustainable and technically difficult to implement.					
33142219	9	57	theme	spot	1367:1370	arg1	0.040 cm2					1377:1385	spot size 0.040 cm2	1367:1385	spot size 0.040 cm2	1367:1385	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	57	theme	spot	1367:1370	arg1	λ = 660 nm					1351:1360	λ = 660 nm	1351:1360	λ = 660 nm	1351:1360	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	7	58	dep	production	1026:1035	arg1	the					1022:1024	the	1022:1024	the	1022:1024	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	0	59	dep	spectroscopy	178:189	arg1	means					163:167	means	163:167	means	163:167	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	8	60	theme	qualitative	1097:1107	arg1	evaluation					1126:1135	qualitative and quantitative evaluation	1097:1135	qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation	1097:1207	Raman spectroscopy has been used for qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation.					
33142219	4	61	theme	enhanced	593:600	arg1	MEOR					616:619	MEOR	616:619	MEOR	616:619	In this context, microbial enhanced oil recovery (MEOR) represents an attractive alternative.					
33142219	4	61	theme	enhanced	593:600	arg1	recovery					606:613	microbial enhanced oil recovery	583:613	microbial enhanced oil recovery (MEOR)	583:620	In this context, microbial enhanced oil recovery (MEOR) represents an attractive alternative.					
33142219	11	62	theme	linear	1684:1689	arg1	GLM					1698:1700	GLM	1698:1700	GLM	1698:1700	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	11	62	theme	linear	1684:1689	arg1	model					1691:1695	general linear model	1676:1695	general linear model (GLM)	1676:1701	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	0	63	theme	distilled	110:118	arg1	water					120:124	distilled water	110:124	distilled water	110:124	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	14	64	theme	bacterial	2236:2244	arg1	production					2246:2255	bacterial production	2236:2255	bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction	2236:2374	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	11	65	theme	dialysis-produced	1889:1905	arg1	water					1907:1911	dialysis-produced water	1889:1911	dialysis-produced water	1889:1911	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	8	66	theme	quantitative	1113:1124	arg1	evaluation					1126:1135	qualitative and quantitative evaluation	1097:1135	qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation	1097:1207	Raman spectroscopy has been used for qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation.					
33142219	9	67	dep	irradiation	1338:1348	arg1	0.040 cm2					1377:1385	spot size 0.040 cm2	1367:1385	spot size 0.040 cm2	1367:1385	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	67	dep	irradiation	1338:1348	arg1	8.0 J/cm2					1402:1410	8.0 J/cm2	1402:1410	8.0 J/cm2	1402:1410	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	67	dep	irradiation	1338:1348	arg1	λ = 660 nm					1351:1360	λ = 660 nm	1351:1360	λ = 660 nm	1351:1360	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	67	dep	irradiation	1338:1348	arg1	CW					1363:1364	CW	1363:1364	CW	1363:1364	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	67	dep	irradiation	1338:1348	arg1	40 mW					1388:1392	40 mW	1388:1392	40 mW	1388:1392	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	67	dep	irradiation	1338:1348	arg1	444 s					1395:1399	444 s	1395:1399	444 s	1395:1399	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	8	68	theme	light	1191:1195	arg1	stimulation					1197:1207	light stimulation	1191:1207	light stimulation	1191:1207	Raman spectroscopy has been used for qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation.					
33142219	11	69	theme	ANOVA	1670:1674	arg1	Results					1630:1636	Results	1630:1636	Results of the exploratory analysis and ANOVA general linear model (GLM)	1630:1701	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	8	70	theme	biopolymer	1174:1183	arg1	composition					1156:1166	the biochemical composition	1140:1166	the biochemical composition of XG biopolymer under light stimulation	1140:1207	Raman spectroscopy has been used for qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation.					
33142219	7	71	from	water	941:945	arg1	photo-stimulation					973:989	combination photo-stimulation	961:989	combination photo-stimulation with laser/LED	961:1004	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	13	72	theme	pyruvate	2015:2022	arg1	content					2039:2045	pyruvate acetyl mannose content	2015:2045	pyruvate acetyl mannose content	2015:2045	In contrast, both pyruvate acetyl mannose content went up in the dialysis-water culture.					
33142219	7	73	theme	water	941:945	arg1	use					925:927	the use	921:927	the use of produced water (PW) water in combination photo-stimulation with laser/LED	921:1004	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	8	74	theme	biochemical	1144:1154	arg1	composition					1156:1166	the biochemical composition	1140:1166	the biochemical composition of XG biopolymer under light stimulation	1140:1207	Raman spectroscopy has been used for qualitative and quantitative evaluation of the biochemical composition of XG biopolymer under light stimulation.					
33142219	6	75	theme	well-known	871:880	arg1	XG					895:896	XG	895:896	XG	895:896	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	6	75	theme	well-known	871:880	arg1	gum					890:892	the well-known Xanthan gum	867:892	the well-known Xanthan gum (XG)	867:897	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	1	76	from	one	226:228	arg1	matrix					302:307	the world and world's energy matrix	273:307	the world and world's energy matrix for the foreseeable future	273:334	Oil is expected to continue to be one of the most important sources of energy in the world and world's energy matrix for the foreseeable future.					
33142219	13	77	dep	content	2039:2045	arg1	both					2010:2013	both	2010:2013	both	2010:2013	In contrast, both pyruvate acetyl mannose content went up in the dialysis-water culture.					
33142219	14	78	from	benefit	2302:2308	arg1	implementation					2317:2330	the implementation	2313:2330	the implementation of sustainable practices for oil extraction	2313:2374	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	0	79	theme	produced	145:152	arg1	water					154:158	dialyzed or not produced water	129:158	dialyzed or not produced water	129:158	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	11	80	theme	distilled	1839:1847	arg1	water					1849:1853	distilled water	1839:1853	distilled water	1839:1853	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	4	81	theme	attractive	636:645	arg1	alternative					647:657	an attractive alternative	633:657	an attractive alternative	633:657	In this context, microbial enhanced oil recovery (MEOR) represents an attractive alternative.					
33142219	9	82	theme	X.	1210:1211	arg1	cultures					1224:1231	X. campestris cultures	1210:1231	X. campestris cultures in either distilled water or dialysis-produced water	1210:1284	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	0	83	theme	Xanthan	69:75	arg1	gum					77:79	Xanthan gum	69:79	Xanthan gum	69:79	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	1	84	theme	energy	295:300	arg1	matrix					302:307	the world and world's energy matrix	273:307	the world and world's energy matrix for the foreseeable future	273:334	Oil is expected to continue to be one of the most important sources of energy in the world and world's energy matrix for the foreseeable future.					
33142219	2	85	theme	oil	406:408	arg1	fields					410:415	oil fields	406:415	oil fields	406:415	However, high demand for energy and the decline of the production of oil fields makes oil recovery a challenge.					
33142219	12	86	theme	XG	1941:1942	arg1	production					1944:1953	XG production	1941:1953	XG production	1941:1953	XG production increased in the distilled water culture.					
33142219	11	87	theme	analysis	1657:1664	arg1	Results					1630:1636	Results	1630:1636	Results of the exploratory analysis and ANOVA general linear model (GLM)	1630:1701	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	3	88	theme	recovery	478:485	arg1	process					487:493	the recovery process	474:493	the recovery process	474:493	Most techniques used for the recovery process are expensive, non-sustainable and technically difficult to implement.					
33142219	9	89	dep	LED	1416:1418	arg1	λ = 630 nm ± 2 nm					1421:1437	λ = 630 nm ± 2 nm	1421:1437	λ = 630 nm ± 2 nm	1421:1437	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	89	dep	LED	1416:1418	arg1	CW					1440:1441	CW	1440:1441	CW	1440:1441	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	89	dep	LED	1416:1418	arg1	140 mW					1464:1469	140 mW	1464:1469	140 mW	1464:1469	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	89	dep	LED	1416:1418	arg1	500 s					1472:1476	500 s	1472:1476	500 s	1472:1476	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	89	dep	LED	1416:1418	arg1	0.50 cm2					1454:1461	spot size 0.50 cm2	1444:1461	spot size 0.50 cm2	1444:1461	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	89	dep	LED	1416:1418	arg1	12 J/cm2					1479:1486	12 J/cm2	1479:1486	12 J/cm2	1479:1486	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	14	90	theme	cultures	2164:2171	arg1	use					2142:2144	the use	2138:2144	the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction	2138:2374	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	12	91	theme	water	1982:1986	arg1	culture					1988:1994	the distilled water culture	1968:1994	the distilled water culture	1968:1994	XG production increased in the distilled water culture.					
33142219	9	92	theme	size	1449:1452	arg1	λ = 630 nm ± 2 nm					1421:1437	λ = 630 nm ± 2 nm	1421:1437	λ = 630 nm ± 2 nm	1421:1437	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	92	theme	size	1449:1452	arg1	0.50 cm2					1454:1461	spot size 0.50 cm2	1444:1461	spot size 0.50 cm2	1444:1461	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	9	93	theme	dialysis-produced	1262:1278	arg1	water					1280:1284	dialysis-produced water	1262:1284	dialysis-produced water	1262:1284	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	14	94	dep	XG	2277:2278	arg1	i.e.					2271:2274	i.e.	2271:2274	i.e.	2271:2274	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	11	95	theme	pyruvyl	1749:1755	arg1	production					1766:1775	pyruvate (pyruvyl mannose) production	1739:1775	pyruvate (pyruvyl mannose) production	1739:1775	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	0	96	contain	containing	99:108	arg2	water					154:158	dialyzed or not produced water	129:158	dialyzed or not produced water	129:158	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	0	96	contain	containing	99:108	arg2	water					120:124	distilled water	110:124	distilled water	110:124	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	0	96	contain	containing	99:108	arg1	media					93:97	media	93:97	media containing distilled water or dialyzed or not produced water	93:158	Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy.					
33142219	1	97	theme	sources	252:258	arg1	sources					252:258	the most important sources	233:258	the most important sources of energy	233:268	Oil is expected to continue to be one of the most important sources of energy in the world and world's energy matrix for the foreseeable future.					
33142219	1	97	theme	sources	252:258	arg1	one					226:228	one	226:228	one	226:228	Oil is expected to continue to be one of the most important sources of energy in the world and world's energy matrix for the foreseeable future.					
33142219	7	98	with	photo-stimulation	973:989	arg1	laser/LED					996:1004	laser/LED	996:1004	laser/LED	996:1004	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	2	99	theme	production	392:401	arg1	energy					362:367	energy	362:367	energy	362:367	However, high demand for energy and the decline of the production of oil fields makes oil recovery a challenge.					
33142219	2	99	theme	production	392:401	arg1	decline					377:383	the decline	373:383	the decline of the production of oil fields	373:415	However, high demand for energy and the decline of the production of oil fields makes oil recovery a challenge.					
33142219	6	100	dep	campestris	796:805	arg1	e.g.					778:781	e.g.	778:781	e.g.	778:781	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	10	101	theme	Raman	1536:1540	arg1	spectroscopy					1542:1553	Raman spectroscopy	1536:1553	Raman spectroscopy at 1064 nm excitation	1536:1575	XG produced by these cultures was analyzed by Raman spectroscopy at 1064 nm excitation and subjected to principal component analysis (PCA).					
33142219	7	102	theme	XG	1056:1057	arg1	composition					1041:1051	composition	1041:1051	composition	1041:1051	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	7	102	theme	XG	1056:1057	arg1	production					1026:1035	production	1026:1035	production	1026:1035	We hypothesized that the use of produced water (PW) water in combination photo-stimulation with laser/LED could influence the production and composition of XG.					
33142219	1	103	theme	energy	263:268	arg1	sources					252:258	the most important sources	233:258	the most important sources of energy	233:268	Oil is expected to continue to be one of the most important sources of energy in the world and world's energy matrix for the foreseeable future.					
33142219	6	104	theme	Xanthomonas	784:794	arg1	campestris					796:805	Xanthomonas campestris	784:805	Xanthomonas campestris	784:805	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	6	104	theme	Xanthomonas	784:794	arg1	species					769:775	Certain bacteria species	752:775	Certain bacteria species (e.g., Xanthomonas campestris)	752:806	Certain bacteria species (e.g., Xanthomonas campestris) produce polysaccharides (exopolysaccharides - EPS) such as the well-known Xanthan gum (XG).					
33142219	14	105	theme	great	2296:2300	arg1	benefit					2302:2308	great benefit	2296:2308	great benefit	2296:2308	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	9	106	theme	laser	1332:1336	arg1	irradiation					1338:1348	laser irradiation	1332:1348	laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2)	1332:1411	X. campestris cultures in either distilled water or dialysis-produced water were studied under the absence or presence of laser irradiation (λ = 660 nm, CW, spot size 0.040 cm2, 40 mW, 444 s, 8.0 J/cm2) or LED (λ = 630 nm ± 2 nm, CW, spot size 0.50 cm2, 140 mW, 500 s, 12 J/cm2).					
33142219	14	107	theme	wide	2107:2110	arg1	field					2112:2116	a wide field	2105:2116	a wide field of opportunities	2105:2133	These results open a wide field of opportunities in the use of metal-enriched cultures in combination with photo-biomodulation to direct and optimize bacterial production of compounds (i.e., XG) that may be of great benefit in the implementation of sustainable practices for oil extraction.					
33142219	11	108	theme	pyruvate	1739:1746	arg1	production					1766:1775	pyruvate (pyruvyl mannose) production	1739:1775	pyruvate (pyruvyl mannose) production	1739:1775	Results of the exploratory analysis and ANOVA general linear model (GLM) suggested that the extent of XG and pyruvate (pyruvyl mannose) production was affected differentially in X. campestris when cultured in distilled water plus LED photo-stimulation versus dialysis-produced water plus LED photo-stimulation.					
33142219	13	109	theme	dialysis-water	2062:2075	arg1	culture					2077:2083	the dialysis-water culture	2058:2083	the dialysis-water culture	2058:2083	In contrast, both pyruvate acetyl mannose content went up in the dialysis-water culture.					
34476699	0	0	theme	xanthan	99:105	arg1	gum					107:109	xanthan gum	99:109	xanthan gum	99:109	A green, environment-friendly, high-consolidation-strength composite dust suppressant derived from xanthan gum.					
34476699	2	1	theme	solid	651:655	arg1	content					657:663	the optimum solid content	639:663	the optimum solid content	639:663	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	2	1	theme	solid	651:655	arg1	%					670:670	2%	669:670	2%	669:670	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	1	2	theme	poor	159:162	arg1	effect					181:186	poor dust suppression effect	159:186	poor dust suppression effect	159:186	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	7	3	theme	dust	1396:1399	arg1	suppressor					1401:1410	the as-prepared dust suppressor	1380:1410	the as-prepared dust suppressor	1380:1410	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	6	4	theme	coal	1173:1176	arg1	molecules					1178:1186	coal molecules	1173:1186	coal molecules	1173:1186	Furthermore, molecular dynamics simulation reveals not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor.					
34476699	1	5	theme	dust	164:167	arg1	effect					181:186	poor dust suppression effect	159:186	poor dust suppression effect	159:186	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	7	6	with	suppressor	1341:1350	arg1	performance					1367:1377	excellent performance	1357:1377	excellent performance	1357:1377	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	2	7	theme	compressive	487:497	arg1	index					533:537	the main optimization index	511:537	the main optimization index	511:537	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	2	7	theme	compressive	487:497	arg1	strength					499:506	compressive strength	487:506	compressive strength	487:506	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	2	7	theme	compressive	487:497	arg1	indices					590:596	the secondary indices	576:596	the secondary indices	576:596	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	1	8	theme	xanthan	370:376	arg1	XG					383:384	XG	383:384	XG	383:384	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	8	theme	xanthan	370:376	arg1	gum					378:380	xanthan gum	370:380	xanthan gum (XG)	370:385	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	2	9	theme	surface	557:563	arg1	tension					565:571	surface tension	557:571	surface tension	557:571	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	6	10	theme	molecular	1092:1100	arg1	dynamics					1102:1109	molecular dynamics	1092:1109	molecular dynamics simulation	1092:1120	Furthermore, molecular dynamics simulation reveals not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor.					
34476699	1	11	theme	gum	378:380	arg1	polymerization					352:365	the radical polymerization	340:365	the radical polymerization of xanthan gum (XG)	340:385	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	2	12	theme	optimum	603:609	arg1	ratio					611:615	the optimum ratio	599:615	the optimum ratio of MA:VAc	599:625	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	2	12	theme	optimum	603:609	arg1	3:5					631:633	3:5	631:633	3:5	631:633	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	7	13	theme	immense	1429:1435	arg1	suppressor					1341:1350	a multifunctional dust suppressor	1318:1350	a multifunctional dust suppressor with excellent performance	1318:1377	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	7	13	theme	immense	1429:1435	arg1	potential					1437:1445	the immense potential	1425:1445	the immense potential for the control of coal dust	1425:1474	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	5	14	theme	stable	1011:1016	arg1	performance					1018:1028	relatively stable performance	1000:1028	relatively stable performance	1000:1028	The dust suppressant can not only maintain relatively stable performance for a period of time but also degrade naturally.					
34476699	1	15	theme	suppression	169:179	arg1	effect					181:186	poor dust suppression effect	159:186	poor dust suppression effect	159:186	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	4	16	theme	structural	860:869	arg1	integrity					871:879	structural integrity	860:879	structural integrity	860:879	At a wind speed of 10 m/s, the solidified layer still maintained structural integrity, indicating that the dust suppressant exhibits a good dust fixation effect.					
34476699	1	17	theme	vinyl	437:441	arg1	acetate					443:449	vinyl acetate	437:449	vinyl acetate (VAc)	437:455	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	17	theme	vinyl	437:441	arg1	suppressor					310:319	higher-consolidation-strength composite dust suppressor	265:319	higher-consolidation-strength composite dust suppressor	265:319	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	17	theme	vinyl	437:441	arg1	greener					253:259	a greener	251:259	a greener	251:259	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	17	theme	vinyl	437:441	arg1	VAc					452:454	VAc	452:454	VAc	452:454	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	7	18	theme	excellent	1357:1365	arg1	performance					1367:1377	excellent performance	1357:1377	excellent performance	1357:1377	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	1	19	theme	effect	181:186	arg1	issues					121:126	issues	121:126	issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors	121:248	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	0	20	theme	green	2:6	arg1	suppressant					74:84	A green, environment-friendly, high-consolidation-strength composite dust suppressant	0:84	A green, environment-friendly, high-consolidation-strength composite dust suppressant	0:84	A green, environment-friendly, high-consolidation-strength composite dust suppressant derived from xanthan gum.					
34476699	7	21	theme	as-prepared	1384:1394	arg1	suppressor					1401:1410	the as-prepared dust suppressor	1380:1410	the as-prepared dust suppressor	1380:1410	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	2	22	theme	secondary	580:588	arg1	strength					499:506	compressive strength	487:506	compressive strength	487:506	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	2	22	theme	secondary	580:588	arg1	indices					590:596	the secondary indices	576:596	the secondary indices	576:596	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	2	23	theme	VAc	623:625	arg1	ratio					611:615	the optimum ratio	599:615	the optimum ratio of MA:VAc	599:625	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	2	23	theme	VAc	623:625	arg1	3:5					631:633	3:5	631:633	3:5	631:633	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	7	24	theme	dust	1336:1339	arg1	suppressor					1341:1350	a multifunctional dust suppressor	1318:1350	a multifunctional dust suppressor with excellent performance	1318:1377	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	7	24	theme	dust	1336:1339	arg1	potential					1437:1445	the immense potential	1425:1445	the immense potential for the control of coal dust	1425:1474	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	6	25	theme	wetting	1225:1231	arg1	mechanism					1233:1241	the wetting mechanism	1221:1241	not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor	1130:1264	Furthermore, molecular dynamics simulation reveals not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor.					
34476699	4	26	theme	good	930:933	arg1	effect					949:954	a good dust fixation effect	928:954	a good dust fixation effect	928:954	At a wind speed of 10 m/s, the solidified layer still maintained structural integrity, indicating that the dust suppressant exhibits a good dust fixation effect.					
34476699	1	27	theme	higher-consolidation-strength	265:293	arg1	substrate					400:408	the graft substrate	390:408	the graft substrate	390:408	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	27	theme	higher-consolidation-strength	265:293	arg1	suppressor					310:319	higher-consolidation-strength composite dust suppressor	265:319	higher-consolidation-strength composite dust suppressor	265:319	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	27	theme	higher-consolidation-strength	265:293	arg1	acrylate					418:425	methyl acrylate	411:425	methyl acrylate (MA)	411:430	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	27	theme	higher-consolidation-strength	265:293	arg1	acetate					443:449	vinyl acetate	437:449	vinyl acetate (VAc)	437:455	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	27	theme	higher-consolidation-strength	265:293	arg1	greener					253:259	a greener	251:259	a greener	251:259	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	27	theme	higher-consolidation-strength	265:293	arg1	monomers					470:477	the graft monomers	460:477	the graft monomers	460:477	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	28	theme	composite	295:303	arg1	substrate					400:408	the graft substrate	390:408	the graft substrate	390:408	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	28	theme	composite	295:303	arg1	suppressor					310:319	higher-consolidation-strength composite dust suppressor	265:319	higher-consolidation-strength composite dust suppressor	265:319	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	28	theme	composite	295:303	arg1	acrylate					418:425	methyl acrylate	411:425	methyl acrylate (MA)	411:430	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	28	theme	composite	295:303	arg1	acetate					443:449	vinyl acetate	437:449	vinyl acetate (VAc)	437:455	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	28	theme	composite	295:303	arg1	greener					253:259	a greener	251:259	a greener	251:259	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	28	theme	composite	295:303	arg1	monomers					470:477	the graft monomers	460:477	the graft monomers	460:477	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	2	29	theme	main	515:518	arg1	index					533:537	the main optimization index	511:537	the main optimization index	511:537	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	2	29	theme	main	515:518	arg1	strength					499:506	compressive strength	487:506	compressive strength	487:506	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	1	30	theme	secondary	193:201	arg1	pollution					203:211	secondary pollution	193:211	secondary pollution	193:211	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	4	31	theme	fixation	940:947	arg1	effect					949:954	a good dust fixation effect	928:954	a good dust fixation effect	928:954	At a wind speed of 10 m/s, the solidified layer still maintained structural integrity, indicating that the dust suppressant exhibits a good dust fixation effect.					
34476699	1	32	theme	dust	305:308	arg1	substrate					400:408	the graft substrate	390:408	the graft substrate	390:408	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	32	theme	dust	305:308	arg1	suppressor					310:319	higher-consolidation-strength composite dust suppressor	265:319	higher-consolidation-strength composite dust suppressor	265:319	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	32	theme	dust	305:308	arg1	acrylate					418:425	methyl acrylate	411:425	methyl acrylate (MA)	411:430	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	32	theme	dust	305:308	arg1	acetate					443:449	vinyl acetate	437:449	vinyl acetate (VAc)	437:455	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	32	theme	dust	305:308	arg1	greener					253:259	a greener	251:259	a greener	251:259	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	32	theme	dust	305:308	arg1	monomers					470:477	the graft monomers	460:477	the graft monomers	460:477	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	6	33	theme	dust	1250:1253	arg1	suppressor					1255:1264	the dust suppressor	1246:1264	the dust suppressor	1246:1264	Furthermore, molecular dynamics simulation reveals not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor.					
34476699	7	34	theme	multifunctional	1320:1334	arg1	suppressor					1341:1350	a multifunctional dust suppressor	1318:1350	a multifunctional dust suppressor with excellent performance	1318:1377	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	7	34	theme	multifunctional	1320:1334	arg1	potential					1437:1445	the immense potential	1425:1445	the immense potential for the control of coal dust	1425:1474	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	1	35	theme	pollution	203:211	arg1	issues					121:126	issues	121:126	issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors	121:248	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	5	36	theme	time	1046:1049	arg1	period					1036:1041	a period	1034:1041	a period of time	1034:1049	The dust suppressant can not only maintain relatively stable performance for a period of time but also degrade naturally.					
34476699	4	37	theme	dust	902:905	arg1	suppressant					907:917	the dust suppressant	898:917	the dust suppressant	898:917	At a wind speed of 10 m/s, the solidified layer still maintained structural integrity, indicating that the dust suppressant exhibits a good dust fixation effect.					
34476699	3	38	theme	prepared	701:708	arg1	suppressant					715:725	the prepared dust suppressant	697:725	the prepared dust suppressant	697:725	Experiments reveal that the prepared dust suppressant can naturally infiltrate into coal to form a hard solidified layer.					
34476699	4	39	theme	solidified	826:835	arg1	layer					837:841	the solidified layer	822:841	the solidified layer	822:841	At a wind speed of 10 m/s, the solidified layer still maintained structural integrity, indicating that the dust suppressant exhibits a good dust fixation effect.					
34476699	1	40	theme	graft	394:398	arg1	suppressor					310:319	higher-consolidation-strength composite dust suppressor	265:319	higher-consolidation-strength composite dust suppressor	265:319	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	40	theme	graft	394:398	arg1	substrate					400:408	the graft substrate	390:408	the graft substrate	390:408	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	40	theme	graft	394:398	arg1	greener					253:259	a greener	251:259	a greener	251:259	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	6	41	theme	interaction	1143:1153	arg1	mechanism					1155:1163	the interaction mechanism	1139:1163	not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor	1130:1264	Furthermore, molecular dynamics simulation reveals not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor.					
34476699	2	42	theme	optimum	643:649	arg1	content					657:663	the optimum solid content	639:663	the optimum solid content	639:663	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	2	42	theme	optimum	643:649	arg1	%					670:670	2%	669:670	2%	669:670	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	0	43	theme	dust	69:72	arg1	suppressant					74:84	A green, environment-friendly, high-consolidation-strength composite dust suppressant	0:84	A green, environment-friendly, high-consolidation-strength composite dust suppressant	0:84	A green, environment-friendly, high-consolidation-strength composite dust suppressant derived from xanthan gum.					
34476699	3	44	theme	dust	710:713	arg1	suppressant					715:725	the prepared dust suppressant	697:725	the prepared dust suppressant	697:725	Experiments reveal that the prepared dust suppressant can naturally infiltrate into coal to form a hard solidified layer.					
34476699	7	45	theme	dust	1471:1474	arg1	control					1455:1461	the control	1451:1461	the control of coal dust	1451:1474	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	0	46	theme	composite	59:67	arg1	suppressant					74:84	A green, environment-friendly, high-consolidation-strength composite dust suppressant	0:84	A green, environment-friendly, high-consolidation-strength composite dust suppressant	0:84	A green, environment-friendly, high-consolidation-strength composite dust suppressant derived from xanthan gum.					
34476699	6	47	theme	suppressor	1255:1264	arg1	mechanism					1233:1241	the wetting mechanism	1221:1241	not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor	1130:1264	Furthermore, molecular dynamics simulation reveals not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor.					
34476699	6	47	theme	suppressor	1255:1264	arg1	mechanism					1155:1163	the interaction mechanism	1139:1163	not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor	1130:1264	Furthermore, molecular dynamics simulation reveals not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor.					
34476699	4	48	theme	wind	800:803	arg1	speed					805:809	a wind speed	798:809	a wind speed of 10 m/s	798:819	At a wind speed of 10 m/s, the solidified layer still maintained structural integrity, indicating that the dust suppressant exhibits a good dust fixation effect.					
34476699	3	49	theme	solidified	777:786	arg1	layer					788:792	a hard solidified layer	770:792	a hard solidified layer	770:792	Experiments reveal that the prepared dust suppressant can naturally infiltrate into coal to form a hard solidified layer.					
34476699	7	50	theme	Experimental	1267:1278	arg1	results					1295:1301	Experimental and simulation results	1267:1301	results	1295:1301	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	4	51	theme	m/s	817:819	arg1	speed					805:809	a wind speed	798:809	a wind speed of 10 m/s	798:819	At a wind speed of 10 m/s, the solidified layer still maintained structural integrity, indicating that the dust suppressant exhibits a good dust fixation effect.					
34476699	1	52	theme	methyl	411:416	arg1	greener					253:259	a greener	251:259	a greener	251:259	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	52	theme	methyl	411:416	arg1	MA					428:429	MA	428:429	MA	428:429	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	52	theme	methyl	411:416	arg1	suppressor					310:319	higher-consolidation-strength composite dust suppressor	265:319	higher-consolidation-strength composite dust suppressor	265:319	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	52	theme	methyl	411:416	arg1	acrylate					418:425	methyl acrylate	411:425	methyl acrylate (MA)	411:430	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	53	theme	graft	464:468	arg1	suppressor					310:319	higher-consolidation-strength composite dust suppressor	265:319	higher-consolidation-strength composite dust suppressor	265:319	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	53	theme	graft	464:468	arg1	greener					253:259	a greener	251:259	a greener	251:259	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	53	theme	graft	464:468	arg1	monomers					470:477	the graft monomers	460:477	the graft monomers	460:477	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	54	theme	low	131:133	arg1	strength					149:156	low consolidation strength	131:156	low consolidation strength	131:156	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	55	theme	current	220:226	arg1	suppressors					238:248	the current coal dust suppressors	216:248	the current coal dust suppressors	216:248	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	2	56	theme	optimization	520:531	arg1	index					533:537	the main optimization index	511:537	the main optimization index	511:537	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	2	56	theme	optimization	520:531	arg1	strength					499:506	compressive strength	487:506	compressive strength	487:506	Taking compressive strength as the main optimization index and viscosity and surface tension as the secondary indices, the optimum ratio of MA:VAc was 3:5 and the optimum solid content was 2%.					
34476699	5	57	theme	dust	961:964	arg1	suppressant					966:976	The dust suppressant	957:976	The dust suppressant	957:976	The dust suppressant can not only maintain relatively stable performance for a period of time but also degrade naturally.					
34476699	7	58	theme	simulation	1284:1293	arg1	results					1295:1301	Experimental and simulation results	1267:1301	results	1295:1301	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	1	59	theme	consolidation	135:147	arg1	strength					149:156	low consolidation strength	131:156	low consolidation strength	131:156	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	60	theme	coal	228:231	arg1	suppressors					238:248	the current coal dust suppressors	216:248	the current coal dust suppressors	216:248	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	8	61	dep	abstract	1487:1494	arg1	Graphical					1477:1485	Graphical	1477:1485	Graphical	1477:1485	Graphical abstract.					
34476699	0	62	dep	green	2:6	arg1	high-consolidation-strength					31:57	high-consolidation-strength	31:57	high-consolidation-strength	31:57	A green, environment-friendly, high-consolidation-strength composite dust suppressant derived from xanthan gum.					
34476699	0	62	dep	green	2:6	arg1	environment-friendly					9:28	environment-friendly	9:28	environment-friendly	9:28	A green, environment-friendly, high-consolidation-strength composite dust suppressant derived from xanthan gum.					
34476699	1	63	theme	strength	149:156	arg1	issues					121:126	issues	121:126	issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors	121:248	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	3	64	theme	hard	772:775	arg1	layer					788:792	a hard solidified layer	770:792	a hard solidified layer	770:792	Experiments reveal that the prepared dust suppressant can naturally infiltrate into coal to form a hard solidified layer.					
34476699	1	65	theme	dust	233:236	arg1	suppressors					238:248	the current coal dust suppressors	216:248	the current coal dust suppressors	216:248	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	66	theme	radical	344:350	arg1	polymerization					352:365	the radical polymerization	340:365	the radical polymerization of xanthan gum (XG)	340:385	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	6	67	theme	dynamics	1102:1109	arg1	simulation					1111:1120	molecular dynamics simulation	1092:1120	molecular dynamics simulation	1092:1120	Furthermore, molecular dynamics simulation reveals not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor.					
34476699	7	68	theme	coal	1466:1469	arg1	dust					1471:1474	coal dust	1466:1474	coal dust	1466:1474	Experimental and simulation results reveal that as a multifunctional dust suppressor with excellent performance, the as-prepared dust suppressor demonstrates the immense potential for the control of coal dust.					
34476699	4	69	theme	dust	935:938	arg1	effect					949:954	a good dust fixation effect	928:954	a good dust fixation effect	928:954	At a wind speed of 10 m/s, the solidified layer still maintained structural integrity, indicating that the dust suppressant exhibits a good dust fixation effect.					
34476699	6	70	theme	dust	1196:1199	arg1	suppressor					1201:1210	the dust suppressor	1192:1210	the dust suppressor	1192:1210	Furthermore, molecular dynamics simulation reveals not only the interaction mechanism between coal molecules and the dust suppressor but also the wetting mechanism of the dust suppressor.					
34476699	1	71	theme	suppressors	238:248	arg1	pollution					203:211	secondary pollution	193:211	secondary pollution	193:211	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	71	theme	suppressors	238:248	arg1	effect					181:186	poor dust suppression effect	159:186	poor dust suppression effect	159:186	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
34476699	1	71	theme	suppressors	238:248	arg1	strength					149:156	low consolidation strength	131:156	low consolidation strength	131:156	To solve issues of low consolidation strength, poor dust suppression effect, and secondary pollution of the current coal dust suppressors, a greener and higher-consolidation-strength composite dust suppressor was synthesized by the radical polymerization of xanthan gum (XG) as the graft substrate, methyl acrylate (MA), and vinyl acetate (VAc) as the graft monomers.					
33892027	0	0	theme	effect	93:98	arg1	study					80:84	Modelling and experimental study	53:84	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.	0:154	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	5	1	theme	gel	1113:1115	arg1	fraction					1117:1124	62.86 ± 1.11% gel fraction	1099:1124	62.86 ± 1.11% gel fraction	1099:1124	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	2	theme	mesh	1003:1006	arg1	size					1008:1011	25.65 ± 1.86 nm mesh size	987:1011	25.65 ± 1.86 nm mesh size	987:1011	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	3	theme	good	1181:1184	arg1	agreement					1186:1194	good agreement	1181:1194	good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure	1181:1293	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	3	4	theme	linear	688:693	arg1	regression					695:704	multiple linear regression	679:704	multiple linear regression	679:704	Second, best fit values for the responses are obtained using multiple linear regression and MATLAB R2020a curve fitting and predictive models are generated.					
33892027	5	5	theme	nm	1000:1001	arg1	size					1008:1011	25.65 ± 1.86 nm mesh size	987:1011	25.65 ± 1.86 nm mesh size	987:1011	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	0	6	theme	composition	103:113	arg1	effect					93:98	the effect	89:98	the effect of composition on microstructure and swelling response	89:153	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	3	7	theme	predictive	742:751	arg1	models					753:758	predictive models	742:758	predictive models	742:758	Second, best fit values for the responses are obtained using multiple linear regression and MATLAB R2020a curve fitting and predictive models are generated.					
33892027	4	8	theme	thermal	916:922	arg1	sensitivity					924:934	thermal sensitivity	916:934	thermal sensitivity	916:934	Third, the optimum microstructure is loaded with polyethylene glycol (PEG) and bismuth telluride (Bi2Te3) and coated on fabric for imparting thermal sensitivity.					
33892027	3	9	theme	Second	618:623	arg1	values					635:640	Second, best fit values	618:640	Second, best fit values for the responses	618:658	Second, best fit values for the responses are obtained using multiple linear regression and MATLAB R2020a curve fitting and predictive models are generated.					
33892027	5	10	located	found	1130:1134	arg1	CMC					1139:1141	CMC	1139:1141	CMC	1139:1141	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	10	located	found	1130:1134	arg2	microstructure					971:984	(1) optimum microstructure	959:984	(1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction)	959:1125	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	11	theme	±	993:993	arg1	size					1008:1011	25.65 ± 1.86 nm mesh size	987:1011	25.65 ± 1.86 nm mesh size	987:1011	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	2	12	theme	CMC	317:319	arg1	hydrogels					337:345	carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels	292:345	carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid	292:375	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	6	13	theme	CMC-CSN	1487:1493	arg1	G3					1508:1509	G3	1508:1509	G3	1508:1509	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	6	13	theme	CMC-CSN	1487:1493	arg1	swelling					1495:1502	CMC-CSN swelling	1487:1502	CMC-CSN swelling	1487:1502	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	6	13	theme	CMC-CSN	1487:1493	arg1	matrix					1528:1533	an ideal host matrix	1514:1533	an ideal host matrix for wearable textiles/devices	1514:1563	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	2	14	theme	microstructure	547:560	arg1	parameters					562:571	microstructure parameters	547:571	microstructure parameters	547:571	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	5	15	theme	62.86	1099:1103	arg1	fraction					1117:1124	62.86 ± 1.11% gel fraction	1099:1124	62.86 ± 1.11% gel fraction	1099:1124	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	6	16	theme	wearable	1539:1546	arg1	textiles/devices					1548:1563	wearable textiles/devices	1539:1563	wearable textiles/devices	1539:1563	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	5	17	theme	±	1105:1105	arg1	fraction					1117:1124	62.86 ± 1.11% gel fraction	1099:1124	62.86 ± 1.11% gel fraction	1099:1124	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	18	theme	elevated	1390:1397	arg1	temperatures					1399:1410	elevated temperatures	1390:1410	elevated temperatures	1390:1410	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	19	dep	size	1008:1011	arg1	swelling					1085:1092	348.03 ± 10.81% swelling	1069:1092	348.03 ± 10.81% swelling	1069:1092	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	19	dep	size	1008:1011	arg1	crosslinking-density					1047:1066	116.25 ± 0.00 μmol/cm3 effective crosslinking-density	1014:1066	116.25 ± 0.00 μmol/cm3 effective crosslinking-density	1014:1066	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	19	dep	size	1008:1011	arg1	fraction					1117:1124	62.86 ± 1.11% gel fraction	1099:1124	62.86 ± 1.11% gel fraction	1099:1124	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	2	20	theme	cellulose	306:314	arg1	hydrogels					337:345	carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels	292:345	carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid	292:375	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	0	21	theme	cellulose-chitosan	14:31	arg1	hydrogel					43:50	Carboxymethyl cellulose-chitosan composite hydrogel	0:50	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.	0:154	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	4	22	theme	optimum	786:792	arg1	microstructure					794:807	the optimum microstructure	782:807	the optimum microstructure	782:807	Third, the optimum microstructure is loaded with polyethylene glycol (PEG) and bismuth telluride (Bi2Te3) and coated on fabric for imparting thermal sensitivity.					
33892027	6	23	theme	model	1467:1471	arg1	potential					1440:1448	the potential	1436:1448	the potential of the generated model	1436:1471	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	0	24	theme	Carboxymethyl	0:12	arg1	hydrogel					43:50	Carboxymethyl cellulose-chitosan composite hydrogel	0:50	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.	0:154	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	3	25	theme	R2020a	717:722	arg1	fitting					730:736	MATLAB R2020a curve fitting	710:736	MATLAB R2020a curve fitting	710:736	Second, best fit values for the responses are obtained using multiple linear regression and MATLAB R2020a curve fitting and predictive models are generated.					
33892027	5	26	theme	fabric	1361:1366	arg1	conductivity					1345:1356	thermal conductivity	1337:1356	thermal conductivity of fabric	1337:1366	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	2	27	theme	CSN	332:334	arg1	hydrogels					337:345	carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels	292:345	carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid	292:375	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	6	28	theme	generated	1457:1465	arg1	model					1467:1471	the generated model	1453:1471	the generated model	1453:1471	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	5	29	dep	shows	1175:1179	arg1	2					1162:1162	2	1162:1162	2	1162:1162	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	0	30	theme	swelling	137:144	arg1	response					146:153	swelling response	137:153	swelling response	137:153	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	5	31	dep	G3/PEG	1304:1309	arg1	3					1301:1301	3	1301:1301	3	1301:1301	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	1	32	theme	functional	214:223	arg1	hydrogels					262:270	functional supramolecular natural polymer-based hydrogels	214:270	functional supramolecular natural polymer-based hydrogels	214:270	Molecular recognition is essential for the advancement of functional supramolecular natural polymer-based hydrogels.					
33892027	0	33	theme	composite	33:41	arg1	hydrogel					43:50	Carboxymethyl cellulose-chitosan composite hydrogel	0:50	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.	0:154	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	5	34	theme	optimum	963:969	arg1	microstructure					971:984	(1) optimum microstructure	959:984	(1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction)	959:1125	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	0	35	from	effect	93:98	arg1	response					146:153	swelling response	137:153	swelling response	137:153	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	0	35	from	effect	93:98	arg1	microstructure					118:131	microstructure	118:131	microstructure	118:131	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	2	36	theme	-chitosan	321:329	arg1	hydrogels					337:345	carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels	292:345	carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid	292:375	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	5	37	theme	hydrogel	1258:1265	arg1	swelling					1267:1274	hydrogel swelling	1258:1274	hydrogel swelling	1258:1274	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	1	38	theme	supramolecular	225:238	arg1	hydrogels					262:270	functional supramolecular natural polymer-based hydrogels	214:270	functional supramolecular natural polymer-based hydrogels	214:270	Molecular recognition is essential for the advancement of functional supramolecular natural polymer-based hydrogels.					
33892027	0	39	theme	Modelling	53:61	arg1	study					80:84	Modelling and experimental study	53:84	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.	0:154	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	5	40	theme	%	1083:1083	arg1	swelling					1085:1092	348.03 ± 10.81% swelling	1069:1092	348.03 ± 10.81% swelling	1069:1092	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	41	theme	±	1021:1021	arg1	crosslinking-density					1047:1066	116.25 ± 0.00 μmol/cm3 effective crosslinking-density	1014:1066	116.25 ± 0.00 μmol/cm3 effective crosslinking-density	1014:1066	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	1	42	theme	natural	240:246	arg1	hydrogels					262:270	functional supramolecular natural polymer-based hydrogels	214:270	functional supramolecular natural polymer-based hydrogels	214:270	Molecular recognition is essential for the advancement of functional supramolecular natural polymer-based hydrogels.					
33892027	2	43	theme	physicochemical	577:591	arg1	properties					593:602	physicochemical properties	577:602	physicochemical properties	577:602	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	3	44	theme	multiple	679:686	arg1	regression					695:704	multiple linear regression	679:704	multiple linear regression	679:704	Second, best fit values for the responses are obtained using multiple linear regression and MATLAB R2020a curve fitting and predictive models are generated.					
33892027	4	45	theme	bismuth	854:860	arg1	telluride					862:870	bismuth telluride	854:870	bismuth telluride (Bi2Te3)	854:879	Third, the optimum microstructure is loaded with polyethylene glycol (PEG) and bismuth telluride (Bi2Te3) and coated on fabric for imparting thermal sensitivity.					
33892027	4	45	theme	bismuth	854:860	arg1	Bi2Te3					873:878	Bi2Te3	873:878	Bi2Te3	873:878	Third, the optimum microstructure is loaded with polyethylene glycol (PEG) and bismuth telluride (Bi2Te3) and coated on fabric for imparting thermal sensitivity.					
33892027	1	46	theme	polymer-based	248:260	arg1	hydrogels					262:270	functional supramolecular natural polymer-based hydrogels	214:270	functional supramolecular natural polymer-based hydrogels	214:270	Molecular recognition is essential for the advancement of functional supramolecular natural polymer-based hydrogels.					
33892027	0	47	theme	experimental	67:78	arg1	study					80:84	Modelling and experimental study	53:84	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.	0:154	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	0	48	from	study	80:84	arg1	response					146:153	swelling response	137:153	swelling response	137:153	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	0	48	from	study	80:84	arg1	microstructure					118:131	microstructure	118:131	microstructure	118:131	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	5	49	theme	μmol/cm3	1028:1035	arg1	crosslinking-density					1047:1066	116.25 ± 0.00 μmol/cm3 effective crosslinking-density	1014:1066	116.25 ± 0.00 μmol/cm3 effective crosslinking-density	1014:1066	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	6	50	theme	host	1523:1526	arg1	G3					1508:1509	G3	1508:1509	G3	1508:1509	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	6	50	theme	host	1523:1526	arg1	swelling					1495:1502	CMC-CSN swelling	1487:1502	CMC-CSN swelling	1487:1502	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	6	50	theme	host	1523:1526	arg1	matrix					1528:1533	an ideal host matrix	1514:1533	an ideal host matrix for wearable textiles/devices	1514:1563	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	2	51	theme	fumaric	364:370	arg1	acid					372:375	fumaric acid	364:375	fumaric acid	364:375	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	2	52	theme	mathematical	478:489	arg1	modelling					491:499	mathematical modelling	478:499	mathematical modelling	478:499	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	5	53	with	agreement	1186:1194	arg1	data					1214:1217	experimental data	1201:1217	experimental data demonstrating potential for estimating hydrogel swelling and microstructure	1201:1293	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	0	54	from	microstructure	118:131	arg1	study					80:84	Modelling and experimental study	53:84	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.	0:154	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	5	55	theme	effective	1037:1045	arg1	crosslinking-density					1047:1066	116.25 ± 0.00 μmol/cm3 effective crosslinking-density	1014:1066	116.25 ± 0.00 μmol/cm3 effective crosslinking-density	1014:1066	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	6	56	theme	ideal	1517:1521	arg1	G3					1508:1509	G3	1508:1509	G3	1508:1509	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	6	56	theme	ideal	1517:1521	arg1	swelling					1495:1502	CMC-CSN swelling	1487:1502	CMC-CSN swelling	1487:1502	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	6	56	theme	ideal	1517:1521	arg1	matrix					1528:1533	an ideal host matrix	1514:1533	an ideal host matrix for wearable textiles/devices	1514:1563	The study demonstrates the potential of the generated model in predicting CMC-CSN swelling and G3 as an ideal host matrix for wearable textiles/devices.					
33892027	5	57	theme	experimental	1201:1212	arg1	data					1214:1217	experimental data	1201:1217	experimental data demonstrating potential for estimating hydrogel swelling and microstructure	1201:1293	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	58	theme	±	1076:1076	arg1	swelling					1085:1092	348.03 ± 10.81% swelling	1069:1092	348.03 ± 10.81% swelling	1069:1092	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	59	theme	0.00	1023:1026	arg1	μmol/cm3					1028:1035	μmol/cm3	1028:1035	μmol/cm3	1028:1035	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	0	60	dep	hydrogel	43:50	arg1	study					80:84	Modelling and experimental study	53:84	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.	0:154	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	2	61	theme	carboxymethyl	292:304	arg1	hydrogels					337:345	carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels	292:345	carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid	292:375	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	2	62	from	influence	400:408	arg1	swelling					447:454	swelling	447:454	swelling	447:454	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	2	62	from	influence	400:408	arg1	microstructure					428:441	microstructure	428:441	microstructure	428:441	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	1	63	theme	hydrogels	262:270	arg1	advancement					199:209	the advancement	195:209	the advancement of functional supramolecular natural polymer-based hydrogels	195:270	Molecular recognition is essential for the advancement of functional supramolecular natural polymer-based hydrogels.					
33892027	4	64	theme	polyethylene	824:835	arg1	PEG					845:847	PEG	845:847	PEG	845:847	Third, the optimum microstructure is loaded with polyethylene glycol (PEG) and bismuth telluride (Bi2Te3) and coated on fabric for imparting thermal sensitivity.					
33892027	4	64	theme	polyethylene	824:835	arg1	glycol					837:842	polyethylene glycol	824:842	polyethylene glycol (PEG)	824:848	Third, the optimum microstructure is loaded with polyethylene glycol (PEG) and bismuth telluride (Bi2Te3) and coated on fabric for imparting thermal sensitivity.					
33892027	3	65	theme	MATLAB	710:715	arg1	fitting					730:736	MATLAB R2020a curve fitting	710:736	MATLAB R2020a curve fitting	710:736	Second, best fit values for the responses are obtained using multiple linear regression and MATLAB R2020a curve fitting and predictive models are generated.					
33892027	5	66	dep	microstructure	971:984	arg1	size					1008:1011	25.65 ± 1.86 nm mesh size	987:1011	25.65 ± 1.86 nm mesh size	987:1011	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	5	66	dep	microstructure	971:984	arg1	1					960:960	1	960:960	1	960:960	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	2	67	theme	hydrogels	337:345	arg1	series					282:287	a series	280:287	a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid	280:375	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	0	68	from	response	146:153	arg1	study					80:84	Modelling and experimental study	53:84	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.	0:154	Carboxymethyl cellulose-chitosan composite hydrogel: Modelling and experimental study of the effect of composition on microstructure and swelling response.					
33892027	2	69	theme	composition	413:423	arg1	influence					400:408	the influence	396:408	the influence of composition on microstructure and swelling	396:454	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	1	70	theme	Molecular	156:164	arg1	recognition					166:176	Molecular recognition	156:176	Molecular recognition	156:176	Molecular recognition is essential for the advancement of functional supramolecular natural polymer-based hydrogels.					
33892027	3	71	theme	curve	724:728	arg1	fitting					730:736	MATLAB R2020a curve fitting	710:736	MATLAB R2020a curve fitting	710:736	Second, best fit values for the responses are obtained using multiple linear regression and MATLAB R2020a curve fitting and predictive models are generated.					
33892027	2	72	theme	hydrolytic	524:533	arg1	properties					535:544	the hydrolytic properties	520:544	the hydrolytic properties	520:544	First, a series of carboxymethyl cellulose (CMC)-chitosan (CSN) hydrogels crosslinked with fumaric acid are studied, where the influence of composition on microstructure and swelling is investigated using mathematical modelling and experiment and the hydrolytic properties, microstructure parameters and physicochemical properties are examined.					
33892027	5	73	theme	%	1111:1111	arg1	fraction					1117:1124	62.86 ± 1.11% gel fraction	1099:1124	62.86 ± 1.11% gel fraction	1099:1124	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
33892027	3	74	dep	Second	618:623	arg1	fit					631:633	fit	631:633	fit	631:633	Second, best fit values for the responses are obtained using multiple linear regression and MATLAB R2020a curve fitting and predictive models are generated.					
33892027	5	75	theme	thermal	1337:1343	arg1	conductivity					1345:1356	thermal conductivity	1337:1356	thermal conductivity of fabric	1337:1366	The results show that (1) optimum microstructure (25.65 ± 1.86 nm mesh size, 116.25 ± 0.00 μmol/cm3 effective crosslinking-density, 348.03 ± 10.81% swelling, and 62.86 ± 1.11% gel fraction) is found at CMC:CSN = 1:3 for G3; (2) the model shows good agreement with experimental data demonstrating potential for estimating hydrogel swelling and microstructure; and (3) G3/PEG and G3/PEG/Bi2Te3 enhance thermal conductivity of fabric at ambient, body, and elevated temperatures.					
34943050	5	0	theme	Glycan	572:577	arg1	structures					579:588	Glycan structures	572:588	Glycan structures	572:588	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	1	1	theme	sufficient	168:177	arg1	oxygenation					185:195	sufficient blood oxygenation	168:195	sufficient blood oxygenation	168:195	Supplemental oxygen is frequently used together with mechanical ventilation to achieve sufficient blood oxygenation.					
34943050	0	2	from	Changes	17:23	arg1	N-Glycome					32:40	the N-Glycome	28:40	the N-Glycome of Murine Pulmonary Endothelial Cells	28:78	Oxygen-Dependent Changes in the N-Glycome of Murine Pulmonary Endothelial Cells.					
34943050	6	3	theme	cell	845:848	arg1	interactions					850:861	cell interactions	845:861	cell interactions	845:861	Altered glycan structures can affect cell interactions and signaling.					
34943050	1	4	theme	blood	179:183	arg1	oxygenation					185:195	sufficient blood oxygenation	168:195	sufficient blood oxygenation	168:195	Supplemental oxygen is frequently used together with mechanical ventilation to achieve sufficient blood oxygenation.					
34943050	8	5	with	approach	1129:1136	arg1	qRT-PCR					1181:1187	qRT-PCR	1181:1187	qRT-PCR	1181:1187	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	8	5	with	approach	1129:1136	arg1	analysis					1202:1209	sialic acid analysis	1190:1209	sialic acid analysis	1190:1209	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	8	5	with	approach	1129:1136	arg1	binding					1222:1228	lectin binding	1215:1228	lectin binding	1215:1228	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	8	5	with	approach	1129:1136	arg1	analysis					1162:1169	N-glycan structure analysis	1143:1169	N-glycan structure analysis by LC-MS	1143:1178	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	3	6	theme	molecular	364:372	arg1	mechanisms					374:383	the molecular mechanisms	360:383	the molecular mechanisms	360:383	Uncertainty is also due to the fact that the molecular mechanisms are still insufficiently understood.					
34943050	6	7	theme	Altered	808:814	arg1	structures					823:832	Altered glycan structures	808:832	Altered glycan structures	808:832	Altered glycan structures can affect cell interactions and signaling.					
34943050	5	8	theme	biosynthetic	696:707	arg1	enzymes					731:737	biosynthetic and glycan remodeling enzymes	696:737	enzymes	731:737	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	5	9	theme	metabolic	639:647	arg1	state					649:653	the metabolic state	635:653	the metabolic state	635:653	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	9	10	from	increase	1439:1446	arg1	response					1481:1488	response	1481:1488	response to hyperoxia	1481:1501	An siRNA approach identified St6gal1 as being primarily responsible for the early transient increase of α2-6 sialylated structures in response to hyperoxia.					
34943050	9	11	theme	sialylated	1456:1465	arg1	structures					1467:1476	α2-6 sialylated structures	1451:1476	α2-6 sialylated structures	1451:1476	An siRNA approach identified St6gal1 as being primarily responsible for the early transient increase of α2-6 sialylated structures in response to hyperoxia.					
34943050	7	12	theme	glycobiology	969:980	arg1	aspects					954:960	aspects	954:960	aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation	954:1064	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	2	13	theme	unpredicted	293:303	arg1	side-effects					305:316	unpredicted side-effects	293:316	unpredicted side-effects	293:316	Despite the undoubted benefits, it is vigorously debated whether too much oxygen can also have unpredicted side-effects.					
34943050	9	14	theme	structures	1467:1476	arg1	increase					1439:1446	the early transient increase	1419:1446	the early transient increase of α2-6 sialylated structures in response to hyperoxia	1419:1501	An siRNA approach identified St6gal1 as being primarily responsible for the early transient increase of α2-6 sialylated structures in response to hyperoxia.					
34943050	8	15	theme	sialic	1190:1195	arg1	acid					1197:1200	sialic acid	1190:1200	sialic acid analysis	1190:1209	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	8	16	theme	global	1313:1318	arg1	glycosylation					1332:1344	global and surface glycosylation	1313:1344	glycosylation	1332:1344	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	4	17	theme	lung	426:429	arg1	endothelium					431:441	The lung endothelium	422:441	The lung endothelium	422:441	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	5	18	dep	level	674:678	arg1	the					659:661	the	659:661	the	659:661	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	7	19	dep	in	1075:1076	arg1	vitro					1078:1082	vitro	1078:1082	vitro	1078:1082	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	5	20	theme	expression	663:672	arg1	level					674:678	expression level	663:678	expression level	663:678	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	6	21	theme	glycan	816:821	arg1	structures					823:832	Altered glycan structures	808:832	Altered glycan structures	808:832	Altered glycan structures can affect cell interactions and signaling.					
34943050	1	22	theme	Supplemental	81:92	arg1	oxygen					94:99	Supplemental oxygen	81:99	Supplemental oxygen	81:99	Supplemental oxygen is frequently used together with mechanical ventilation to achieve sufficient blood oxygenation.					
34943050	8	23	theme	constant	1243:1250	arg1	hyperoxia					1269:1277	constant and intermittent hyperoxia	1243:1277	constant and intermittent hyperoxia	1243:1277	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	8	24	theme	surface	1324:1330	arg1	glycosylation					1332:1344	global and surface glycosylation	1313:1344	glycosylation	1332:1344	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	9	25	theme	siRNA	1350:1354	arg1	approach					1356:1363	An siRNA approach	1347:1363	An siRNA approach	1347:1363	An siRNA approach identified St6gal1 as being primarily responsible for the early transient increase of α2-6 sialylated structures in response to hyperoxia.					
34943050	5	26	theme	redox	782:786	arg1	status					788:793	the redox status	778:793	the redox status of the cell	778:805	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	7	27	theme	in	1075:1076	arg1	system					1097:1102	an in vitro cell culture system	1072:1102	an in vitro cell culture system	1072:1102	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	0	28	theme	Oxygen-Dependent	0:15	arg1	Changes					17:23	Oxygen-Dependent Changes	0:23	Oxygen-Dependent Changes in the N-Glycome of Murine Pulmonary Endothelial Cells	0:78	Oxygen-Dependent Changes in the N-Glycome of Murine Pulmonary Endothelial Cells.					
34943050	8	29	theme	proteomic	1119:1127	arg1	approach					1129:1136	a proteomic approach	1117:1136	a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding	1117:1228	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	7	30	from	emphasis	1019:1026	arg1	N-glycans					1031:1039	N-glycans	1031:1039	N-glycans	1031:1039	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	7	30	from	emphasis	1019:1026	arg1	sialylation					1054:1064	terminal sialylation	1045:1064	terminal sialylation	1045:1064	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	8	31	theme	lectin	1215:1220	arg1	binding					1222:1228	lectin binding	1215:1228	lectin binding	1215:1228	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	2	32	contain	have	288:291	arg2	side-effects					305:316	unpredicted side-effects	293:316	unpredicted side-effects	293:316	Despite the undoubted benefits, it is vigorously debated whether too much oxygen can also have unpredicted side-effects.					
34943050	2	32	contain	have	288:291	arg1	oxygen					272:277	too much oxygen	263:277	too much oxygen	263:277	Despite the undoubted benefits, it is vigorously debated whether too much oxygen can also have unpredicted side-effects.					
34943050	7	33	theme	culture	1089:1095	arg1	system					1097:1102	an in vitro cell culture system	1072:1102	an in vitro cell culture system	1072:1102	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	4	34	theme	broad	476:480	arg1	proteoglycans					521:533	proteoglycans	521:533	proteoglycans	521:533	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	4	34	theme	broad	476:480	arg1	glycocalyx					482:491	an exceptionally broad glycocalyx	459:491	an exceptionally broad glycocalyx	459:491	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	4	34	theme	broad	476:480	arg1	glycosaminoglycans					552:569	glycosaminoglycans	552:569	glycosaminoglycans	552:569	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	4	34	theme	broad	476:480	arg1	glycolipids					536:546	glycolipids	536:546	glycolipids	536:546	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	5	35	theme	cell	802:805	arg1	status					788:793	the redox status	778:793	the redox status of the cell	778:805	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	5	35	theme	cell	802:805	arg1	oxygen					767:772	oxygen	767:772	oxygen	767:772	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	8	36	theme	intermittent	1256:1267	arg1	hyperoxia					1269:1277	constant and intermittent hyperoxia	1243:1277	constant and intermittent hyperoxia	1243:1277	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	8	37	theme	N-glycan	1143:1150	arg1	analysis					1162:1169	N-glycan structure analysis	1143:1169	N-glycan structure analysis by LC-MS	1143:1178	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	7	38	with	glycobiology	969:980	arg1	emphasis					1019:1026	an emphasis	1016:1026	an emphasis on N-glycans and terminal sialylation	1016:1064	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	7	39	theme	cell	1084:1087	arg1	system					1097:1102	an in vitro cell culture system	1072:1102	an in vitro cell culture system	1072:1102	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	8	40	theme	dependent	1292:1300	arg1	changes					1302:1308	time dependent changes	1287:1308	time dependent changes in global and surface glycosylation	1287:1344	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	0	41	theme	Pulmonary	52:60	arg1	Cells					74:78	Murine Pulmonary Endothelial Cells	45:78	Murine Pulmonary Endothelial Cells	45:78	Oxygen-Dependent Changes in the N-Glycome of Murine Pulmonary Endothelial Cells.					
34943050	5	42	theme	glycan	713:718	arg1	enzymes					731:737	biosynthetic and glycan remodeling enzymes	696:737	enzymes	731:737	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	8	43	theme	structure	1152:1160	arg1	analysis					1162:1169	N-glycan structure analysis	1143:1169	N-glycan structure analysis by LC-MS	1143:1178	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	7	44	theme	terminal	1045:1052	arg1	sialylation					1054:1064	terminal sialylation	1045:1064	terminal sialylation	1045:1064	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	1	45	used	used	115:118	arg2	oxygen					94:99	Supplemental oxygen	81:99	Supplemental oxygen	81:99	Supplemental oxygen is frequently used together with mechanical ventilation to achieve sufficient blood oxygenation.					
34943050	0	46	theme	Murine	45:50	arg1	Cells					74:78	Murine Pulmonary Endothelial Cells	45:78	Murine Pulmonary Endothelial Cells	45:78	Oxygen-Dependent Changes in the N-Glycome of Murine Pulmonary Endothelial Cells.					
34943050	5	47	theme	remodeling	720:729	arg1	enzymes					731:737	biosynthetic and glycan remodeling enzymes	696:737	enzymes	731:737	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	7	48	theme	oxygen	933:938	arg1	conditions					940:949	different oxygen conditions	923:949	different oxygen conditions	923:949	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	5	49	theme	enzymes	731:737	arg1	level					674:678	expression level	663:678	expression level	663:678	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	5	49	theme	enzymes	731:737	arg1	state					649:653	the metabolic state	635:653	the metabolic state	635:653	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	5	49	theme	enzymes	731:737	arg1	activity					684:691	activity	684:691	activity	684:691	Glycan structures are not genetically determined but depend on the metabolic state and the expression level and activity of biosynthetic and glycan remodeling enzymes, which can be influenced by oxygen and the redox status of the cell.					
34943050	7	50	theme	pulmonary	989:997	arg1	endothelium					999:1009	the pulmonary endothelium	985:1009	the pulmonary endothelium	985:1009	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	1	51	theme	mechanical	134:143	arg1	ventilation					145:155	mechanical ventilation	134:155	mechanical ventilation	134:155	Supplemental oxygen is frequently used together with mechanical ventilation to achieve sufficient blood oxygenation.					
34943050	7	52	theme	conditions	940:949	arg1	effect					913:918	the effect	909:918	the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation	909:1064	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	7	53	from	effect	913:918	arg1	aspects					954:960	aspects	954:960	aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation	954:1064	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	9	54	theme	early	1423:1427	arg1	increase					1439:1446	the early transient increase	1419:1446	the early transient increase of α2-6 sialylated structures in response to hyperoxia	1419:1501	An siRNA approach identified St6gal1 as being primarily responsible for the early transient increase of α2-6 sialylated structures in response to hyperoxia.					
34943050	8	55	theme	acid	1197:1200	arg1	analysis					1202:1209	sialic acid analysis	1190:1209	sialic acid analysis	1190:1209	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	8	56	from	changes	1302:1308	arg1	glycosylation					1332:1344	global and surface glycosylation	1313:1344	glycosylation	1332:1344	We combined a proteomic approach with N-glycan structure analysis by LC-MS, qRT-PCR, sialic acid analysis and lectin binding to show that constant and intermittent hyperoxia induced time dependent changes in global and surface glycosylation.					
34943050	4	57	contain	carrying	494:501	arg1	proteoglycans					521:533	proteoglycans	521:533	proteoglycans	521:533	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	4	57	contain	carrying	494:501	arg2	O-glycans					510:518	O-glycans	510:518	O-glycans	510:518	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	4	57	contain	carrying	494:501	arg1	glycolipids					536:546	glycolipids	536:546	glycolipids	536:546	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	4	57	contain	carrying	494:501	arg1	glycosaminoglycans					552:569	glycosaminoglycans	552:569	glycosaminoglycans	552:569	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	4	57	contain	carrying	494:501	arg1	glycocalyx					482:491	an exceptionally broad glycocalyx	459:491	an exceptionally broad glycocalyx	459:491	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	4	57	contain	carrying	494:501	arg2	N-					503:504	N-	503:504	N-	503:504	The lung endothelium is covered with an exceptionally broad glycocalyx, carrying N- and O-glycans, proteoglycans, glycolipids and glycosaminoglycans.					
34943050	0	58	theme	Cells	74:78	arg1	N-Glycome					32:40	the N-Glycome	28:40	the N-Glycome of Murine Pulmonary Endothelial Cells	28:78	Oxygen-Dependent Changes in the N-Glycome of Murine Pulmonary Endothelial Cells.					
34943050	2	59	theme	undoubted	210:218	arg1	benefits					220:227	the undoubted benefits	206:227	the undoubted benefits	206:227	Despite the undoubted benefits, it is vigorously debated whether too much oxygen can also have unpredicted side-effects.					
34943050	2	60	theme	much	267:270	arg1	oxygen					272:277	too much oxygen	263:277	too much oxygen	263:277	Despite the undoubted benefits, it is vigorously debated whether too much oxygen can also have unpredicted side-effects.					
34943050	7	61	theme	different	923:931	arg1	conditions					940:949	different oxygen conditions	923:949	different oxygen conditions	923:949	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
34943050	9	62	theme	transient	1429:1437	arg1	increase					1439:1446	the early transient increase	1419:1446	the early transient increase of α2-6 sialylated structures in response to hyperoxia	1419:1501	An siRNA approach identified St6gal1 as being primarily responsible for the early transient increase of α2-6 sialylated structures in response to hyperoxia.					
34943050	0	63	theme	Endothelial	62:72	arg1	Cells					74:78	Murine Pulmonary Endothelial Cells	45:78	Murine Pulmonary Endothelial Cells	45:78	Oxygen-Dependent Changes in the N-Glycome of Murine Pulmonary Endothelial Cells.					
34943050	9	64	gly	sialylated	1456:1465	arg1	structures					1467:1476	α2-6 sialylated structures	1451:1476	α2-6 sialylated structures	1451:1476	An siRNA approach identified St6gal1 as being primarily responsible for the early transient increase of α2-6 sialylated structures in response to hyperoxia.					
34943050	7	65	theme	endothelium	999:1009	arg1	glycobiology					969:980	the glycobiology	965:980	the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation	965:1064	In this study, we investigated the effect of different oxygen conditions on aspects of the glycobiology of the pulmonary endothelium with an emphasis on N-glycans and terminal sialylation using an in vitro cell culture system.					
33194780	6	0	theme	biochemical	850:860	arg1	indexes					862:868	The biochemical indexes	846:868	The biochemical indexes related to inflammation	846:892	The biochemical indexes related to inflammation were determined by ELISA kit, and the therapeutic effect of SCP on UC was clarified.					
33194780	3	1	theme	polysaccharide	515:528	arg1	mechanism					481:489	mechanism	481:489	mechanism	481:489	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	3	1	theme	polysaccharide	515:528	arg1	efficacy					468:475	efficacy	468:475	efficacy	468:475	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	13	2	theme	microbiota	1992:2001	arg1	metabolism					1974:1983	metabolism	1974:1983	metabolism	1974:1983	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
33194780	13	2	theme	microbiota	1992:2001	arg1	composition					1958:1968	composition	1958:1968	composition	1958:1968	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
33194780	1	3	theme	environmental	256:268	arg1	factors					299:305	environmental, microbial, and host-related factors	256:305	environmental, microbial, and host-related factors	256:305	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	9	4	theme	inflammatory	1278:1289	arg1	factors					1291:1297	inflammatory factors	1278:1297	inflammatory factors	1278:1297	Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC.					
33194780	8	5	used	used	1121:1124	arg2	GC-MS					1111:1115	GC-MS	1111:1115	GC-MS	1111:1115	At the same time, GC-MS was used to determine the content of short chain fatty acids in intestinal contents.					
33194780	3	6	theme	disease	575:581	arg1	treatment					543:551	the treatment	539:551	the treatment of inflammatory bowel disease	539:581	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	1	7	theme	bowel	195:199	arg1	IBD					210:212	IBD	210:212	IBD	210:212	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	1	7	theme	bowel	195:199	arg1	disease					201:207	inflammatory bowel disease	182:207	inflammatory bowel disease (IBD)	182:213	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	12	8	theme	gut	1828:1830	arg1	microbiota					1832:1841	gut microbiota	1828:1841	gut microbiota	1828:1841	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	6	9	theme	ELISA	913:917	arg1	kit					919:921	ELISA kit	913:921	ELISA kit	913:921	The biochemical indexes related to inflammation were determined by ELISA kit, and the therapeutic effect of SCP on UC was clarified.					
33194780	7	10	theme	rDNA	989:992	arg1	sequencing					994:1003	16S rDNA sequencing	985:1003	16S rDNA sequencing	985:1003	Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota.					
33194780	3	11	theme	inflammatory	556:567	arg1	disease					575:581	inflammatory bowel disease	556:581	inflammatory bowel disease	556:581	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	5	12	theme	mouse	786:790	arg1	model					792:796	a mouse model	784:796	a mouse model of UC	784:802	Materials/Methods: In this study, we established a mouse model of UC, and used SCP for treatment intervention.					
33194780	8	13	theme	short	1154:1158	arg1	acids					1172:1176	short chain fatty acids	1154:1176	short chain fatty acids	1154:1176	At the same time, GC-MS was used to determine the content of short chain fatty acids in intestinal contents.					
33194780	0	14	theme	Gut	134:136	arg1	Microbiota					138:147	Gut Microbiota	134:147	Gut Microbiota	134:147	The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota.					
33194780	8	15	theme	fatty	1166:1170	arg1	acids					1172:1176	short chain fatty acids	1154:1176	short chain fatty acids	1154:1176	At the same time, GC-MS was used to determine the content of short chain fatty acids in intestinal contents.					
33194780	10	16	theme	UC	1475:1476	arg1	mice					1478:1481	UC mice	1475:1481	UC mice	1475:1481	Results: The results showed that SCP could significantly improve the physiological state of UC mice and regulate the level of inflammatory factors to normal levels.					
33194780	6	17	theme	related	870:876	arg1	indexes					862:868	The biochemical indexes	846:868	The biochemical indexes related to inflammation	846:892	The biochemical indexes related to inflammation were determined by ELISA kit, and the therapeutic effect of SCP on UC was clarified.					
33194780	4	18	theme	induced	694:700	arg1	UC					722:723	UC	722:723	UC	722:723	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	4	18	theme	induced	694:700	arg1	colitis					713:719	induced ulcerative colitis	694:719	induced ulcerative colitis (UC)	694:724	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	11	19	theme	gut	1609:1611	arg1	microbiota					1613:1622	gut microbiota	1609:1622	gut microbiota	1609:1622	Meanwhile, SCP could significantly regulate the imbalance of gut microbiota and increase the content of SCFAs.					
33194780	6	20	theme	SCP	954:956	arg1	effect					944:949	the therapeutic effect	928:949	the therapeutic effect of SCP on UC	928:962	The biochemical indexes related to inflammation were determined by ELISA kit, and the therapeutic effect of SCP on UC was clarified.					
33194780	4	21	theme	sodium	671:676	arg1	DSS					687:689	dextran sodium sulfate (DSS)	663:690	dextran sodium sulfate (DSS)	663:690	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	8	22	from	content	1143:1149	arg1	contents					1192:1199	intestinal contents	1181:1199	intestinal contents	1181:1199	At the same time, GC-MS was used to determine the content of short chain fatty acids in intestinal contents.					
33194780	12	23	with	correlation	1811:1821	arg1	microbiota					1832:1841	gut microbiota	1828:1841	gut microbiota	1828:1841	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	0	24	dep	Composition	104:114	arg1	the					100:102	the	100:102	the	100:102	The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota.					
33194780	2	25	theme	common	415:420	arg1	metabolism					379:388	metabolism	379:388	metabolism	379:388	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	2	25	theme	common	415:420	arg1	composition					363:373	composition	363:373	composition	363:373	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	2	25	theme	common	415:420	arg1	mechanism					422:430	the common mechanism	411:430	the common mechanism of disease resistance	411:452	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	2	26	theme	microbiota	397:406	arg1	metabolism					379:388	metabolism	379:388	metabolism	379:388	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	2	26	theme	microbiota	397:406	arg1	composition					363:373	composition	363:373	composition	363:373	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	2	26	theme	microbiota	397:406	arg1	mechanism					422:430	the common mechanism	411:430	the common mechanism of disease resistance	411:452	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	0	27	theme	Composition	104:114	arg1	Regulation					86:95	the Regulation	82:95	the Regulation of the Composition and Metabolism of Gut Microbiota	82:147	The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota.					
33194780	7	28	theme	gut	1077:1079	arg1	microbiota					1081:1090	gut microbiota	1077:1090	gut microbiota	1077:1090	Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota.					
33194780	12	29	theme	butyric	1748:1754	arg1	acid					1756:1759	butyric acid	1748:1759	butyric acid	1748:1759	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	2	30	theme	disease	435:441	arg1	resistance					443:452	disease resistance	435:452	disease resistance	435:452	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	13	31	theme	IBD	2064:2066	arg1	patients					2068:2075	IBD patients	2064:2075	IBD patients	2064:2075	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
33194780	12	32	theme	gut	1711:1713	arg1	microbiota					1715:1724	gut microbiota	1711:1724	gut microbiota	1711:1724	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	13	33	theme	gut	1988:1990	arg1	microbiota					1992:2001	gut microbiota	1988:2001	gut microbiota	1988:2001	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
33194780	0	34	theme	Schisandra	27:36	arg1	Polysaccharide					48:61	Schisandra chinensis Polysaccharide	27:61	Schisandra chinensis Polysaccharide	27:61	The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota.					
33194780	9	35	theme	gut	1234:1236	arg1	microbiota					1238:1247	gut microbiota	1234:1247	gut microbiota	1234:1247	Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC.					
33194780	4	36	dep	effect	632:637	arg1	the					628:630	the	628:630	the	628:630	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	9	37	from	effect	1348:1353	arg1	UC					1379:1380	UC	1379:1380	UC	1379:1380	Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC.					
33194780	1	38	theme	host-related	286:297	arg1	factors					299:305	environmental, microbial, and host-related factors	256:305	environmental, microbial, and host-related factors	256:305	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	0	39	theme	Polysaccharide	48:61	arg1	Effect					17:22	The Anti-colitis Effect	0:22	The Anti-colitis Effect of Schisandra chinensis Polysaccharide	0:61	The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota.					
33194780	9	40	dep	effect	1348:1353	arg1	the					1344:1346	the	1344:1346	the	1344:1346	Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC.					
33194780	3	41	from	efficacy	468:475	arg1	treatment					543:551	the treatment	539:551	the treatment of inflammatory bowel disease	539:581	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	2	42	theme	host	331:334	arg1	immunity					336:343	host immunity	331:343	host immunity	331:343	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	9	43	theme	chain	1256:1260	arg1	acids					1268:1272	short chain fatty acids	1250:1272	short chain fatty acids	1250:1272	Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC.					
33194780	12	44	theme	correlation	1691:1701	arg1	results					1676:1682	the results	1672:1682	the results of the correlation between gut microbiota and SCFAs	1672:1734	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	4	45	theme	dextran	663:669	arg1	DSS					687:689	dextran sodium sulfate (DSS)	663:690	dextran sodium sulfate (DSS)	663:690	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	4	46	from	DSS	687:689	arg1	mice					729:732	mice	729:732	mice	729:732	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	3	47	dep	efficacy	468:475	arg1	the					464:466	the	464:466	the	464:466	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	3	48	theme	chinensis	505:513	arg1	polysaccharide					515:528	Schisandra chinensis polysaccharide	494:528	Schisandra chinensis polysaccharide (SCP)	494:534	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	3	48	theme	chinensis	505:513	arg1	SCP					531:533	SCP	531:533	SCP	531:533	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	1	49	theme	intricate	231:239	arg1	association					241:251	an intricate association	228:251	an intricate association of environmental, microbial, and host-related factors	228:305	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	3	50	theme	bowel	569:573	arg1	disease					575:581	inflammatory bowel disease	556:581	inflammatory bowel disease	556:581	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	5	51	theme	UC	801:802	arg1	model					792:796	a mouse model	784:796	a mouse model of UC	784:802	Materials/Methods: In this study, we established a mouse model of UC, and used SCP for treatment intervention.					
33194780	7	52	used	used	1009:1012	arg2	sequencing					994:1003	16S rDNA sequencing	985:1003	16S rDNA sequencing	985:1003	Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota.					
33194780	3	53	from	mechanism	481:489	arg1	treatment					543:551	the treatment	539:551	the treatment of inflammatory bowel disease	539:581	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	5	54	used	used	809:812	arg2	we					769:770	we	769:770	we	769:770	Materials/Methods: In this study, we established a mouse model of UC, and used SCP for treatment intervention.					
33194780	4	55	from	mechanism	643:651	arg1	UC					722:723	UC	722:723	UC	722:723	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	4	55	from	mechanism	643:651	arg1	colitis					713:719	induced ulcerative colitis	694:719	induced ulcerative colitis (UC)	694:724	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	4	55	from	mechanism	643:651	arg1	DSS					687:689	dextran sodium sulfate (DSS)	663:690	dextran sodium sulfate (DSS)	663:690	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	1	56	theme	inflammatory	182:193	arg1	IBD					210:212	IBD	210:212	IBD	210:212	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	1	56	theme	inflammatory	182:193	arg1	disease					201:207	inflammatory bowel disease	182:207	inflammatory bowel disease (IBD)	182:213	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	12	57	theme	highest	1803:1809	arg1	correlation					1811:1821	the highest correlation	1799:1821	the highest correlation with gut microbiota	1799:1841	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	9	58	from	mechanism	1359:1367	arg1	UC					1379:1380	UC	1379:1380	UC	1379:1380	Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC.					
33194780	4	59	theme	SCP	656:658	arg1	effect					632:637	effect	632:637	effect	632:637	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	4	59	theme	SCP	656:658	arg1	mechanism					643:651	mechanism	643:651	mechanism	643:651	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	10	60	theme	physiological	1452:1464	arg1	state					1466:1470	the physiological state	1448:1470	the physiological state of UC mice	1448:1481	Results: The results showed that SCP could significantly improve the physiological state of UC mice and regulate the level of inflammatory factors to normal levels.					
33194780	7	61	theme	16S	985:987	arg1	sequencing					994:1003	16S rDNA sequencing	985:1003	16S rDNA sequencing	985:1003	Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota.					
33194780	4	62	from	effect	632:637	arg1	UC					722:723	UC	722:723	UC	722:723	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	4	62	from	effect	632:637	arg1	colitis					713:719	induced ulcerative colitis	694:719	induced ulcerative colitis (UC)	694:724	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	4	62	from	effect	632:637	arg1	DSS					687:689	dextran sodium sulfate (DSS)	663:690	dextran sodium sulfate (DSS)	663:690	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	1	63	theme	disease	201:207	arg1	pathogenesis					166:177	The pathogenesis	162:177	The pathogenesis of inflammatory bowel disease (IBD)	162:213	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	11	64	theme	SCFAs	1652:1656	arg1	content					1641:1647	the content	1637:1647	the content of SCFAs	1637:1656	Meanwhile, SCP could significantly regulate the imbalance of gut microbiota and increase the content of SCFAs.					
33194780	0	65	theme	Microbiota	138:147	arg1	Metabolism					120:129	Metabolism	120:129	Metabolism	120:129	The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota.					
33194780	0	65	theme	Microbiota	138:147	arg1	Composition					104:114	Composition	104:114	Composition	104:114	The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota.					
33194780	1	66	attach	linked	218:223	arg2	pathogenesis					166:177	The pathogenesis	162:177	The pathogenesis of inflammatory bowel disease (IBD)	162:213	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	1	66	attach	linked	218:223	arg1	association					241:251	an intricate association	228:251	an intricate association of environmental, microbial, and host-related factors	228:305	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	8	67	theme	same	1100:1103	arg1	time					1105:1108	the same time	1096:1108	the same time	1096:1108	At the same time, GC-MS was used to determine the content of short chain fatty acids in intestinal contents.					
33194780	13	68	theme	adjuvant	2043:2050	arg1	SCP					2024:2026	SCP	2024:2026	SCP	2024:2026	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
33194780	13	68	theme	adjuvant	2043:2050	arg1	therapy					2052:2058	adjuvant therapy	2043:2058	adjuvant therapy for IBD patients	2043:2075	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
33194780	10	69	theme	mice	1478:1481	arg1	state					1466:1470	the physiological state	1448:1470	the physiological state of UC mice	1448:1481	Results: The results showed that SCP could significantly improve the physiological state of UC mice and regulate the level of inflammatory factors to normal levels.					
33194780	8	70	theme	chain	1160:1164	arg1	acids					1172:1176	short chain fatty acids	1154:1176	short chain fatty acids	1154:1176	At the same time, GC-MS was used to determine the content of short chain fatty acids in intestinal contents.					
33194780	9	71	theme	SCP	1372:1374	arg1	mechanism					1359:1367	mechanism	1359:1367	mechanism	1359:1367	Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC.					
33194780	9	71	theme	SCP	1372:1374	arg1	effect					1348:1353	effect	1348:1353	effect	1348:1353	Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC.					
33194780	8	72	theme	acids	1172:1176	arg1	content					1143:1149	the content	1139:1149	the content of short chain fatty acids in intestinal contents	1139:1199	At the same time, GC-MS was used to determine the content of short chain fatty acids in intestinal contents.					
33194780	11	73	theme	microbiota	1613:1622	arg1	imbalance					1596:1604	the imbalance	1592:1604	the imbalance of gut microbiota	1592:1622	Meanwhile, SCP could significantly regulate the imbalance of gut microbiota and increase the content of SCFAs.					
33194780	8	74	theme	intestinal	1181:1190	arg1	contents					1192:1199	intestinal contents	1181:1199	intestinal contents	1181:1199	At the same time, GC-MS was used to determine the content of short chain fatty acids in intestinal contents.					
33194780	12	75	theme	isobutyric	1762:1771	arg1	acid					1773:1776	isobutyric acid	1762:1776	isobutyric acid	1762:1776	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	5	76	theme	treatment	822:830	arg1	intervention					832:843	treatment intervention	822:843	treatment intervention	822:843	Materials/Methods: In this study, we established a mouse model of UC, and used SCP for treatment intervention.					
33194780	6	77	theme	therapeutic	932:942	arg1	effect					944:949	the therapeutic effect	928:949	the therapeutic effect of SCP on UC	928:962	The biochemical indexes related to inflammation were determined by ELISA kit, and the therapeutic effect of SCP on UC was clarified.					
33194780	0	78	theme	Metabolism	120:129	arg1	Regulation					86:95	the Regulation	82:95	the Regulation of the Composition and Metabolism of Gut Microbiota	82:147	The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota.					
33194780	6	79	from	effect	944:949	arg1	UC					961:962	UC	961:962	UC	961:962	The biochemical indexes related to inflammation were determined by ELISA kit, and the therapeutic effect of SCP on UC was clarified.					
33194780	4	80	theme	sulfate	678:684	arg1	DSS					687:689	dextran sodium sulfate (DSS)	663:690	dextran sodium sulfate (DSS)	663:690	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	13	81	theme	bowel	1926:1930	arg1	disease					1932:1938	inflammatory bowel disease	1913:1938	inflammatory bowel disease	1913:1938	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
33194780	13	82	used	used	2035:2038	arg2	SCP					2024:2026	SCP	2024:2026	SCP	2024:2026	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
33194780	13	82	used	used	2035:2038	arg2	therapy					2052:2058	adjuvant therapy	2043:2058	adjuvant therapy for IBD patients	2043:2075	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
33194780	7	83	theme	microbiota	1081:1090	arg1	diversity					1064:1072	diversity	1064:1072	diversity	1064:1072	Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota.					
33194780	7	83	theme	microbiota	1081:1090	arg1	composition					1048:1058	composition	1048:1058	composition	1048:1058	Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota.					
33194780	2	84	dep	composition	363:373	arg1	the					359:361	the	359:361	the	359:361	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	2	85	theme	gut	393:395	arg1	microbiota					397:406	gut microbiota	393:406	gut microbiota	393:406	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	0	86	theme	Anti-colitis	4:15	arg1	Effect					17:22	The Anti-colitis Effect	0:22	The Anti-colitis Effect of Schisandra chinensis Polysaccharide	0:61	The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota.					
33194780	2	87	theme	resistance	443:452	arg1	metabolism					379:388	metabolism	379:388	metabolism	379:388	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	2	87	theme	resistance	443:452	arg1	composition					363:373	composition	363:373	composition	363:373	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	2	87	theme	resistance	443:452	arg1	mechanism					422:430	the common mechanism	411:430	the common mechanism of disease resistance	411:452	Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance.					
33194780	7	88	dep	composition	1048:1058	arg1	the					1044:1046	the	1044:1046	the	1044:1046	Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota.					
33194780	4	89	theme	ulcerative	702:711	arg1	UC					722:723	UC	722:723	UC	722:723	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	4	89	theme	ulcerative	702:711	arg1	colitis					713:719	induced ulcerative colitis	694:719	induced ulcerative colitis (UC)	694:724	Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice.					
33194780	1	90	theme	microbial	271:279	arg1	factors					299:305	environmental, microbial, and host-related factors	256:305	environmental, microbial, and host-related factors	256:305	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	12	91	contain	had	1795:1797	arg2	correlation					1811:1821	the highest correlation	1799:1821	the highest correlation with gut microbiota	1799:1841	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	12	91	contain	had	1795:1797	arg1	acid					1756:1759	butyric acid	1748:1759	butyric acid	1748:1759	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	12	91	contain	had	1795:1797	arg1	acid					1773:1776	isobutyric acid	1762:1776	isobutyric acid	1762:1776	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	12	91	contain	had	1795:1797	arg1	acid					1790:1793	valeric acid	1782:1793	valeric acid	1782:1793	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	10	92	theme	normal	1533:1538	arg1	levels					1540:1545	normal levels	1533:1545	normal levels	1533:1545	Results: The results showed that SCP could significantly improve the physiological state of UC mice and regulate the level of inflammatory factors to normal levels.					
33194780	0	93	theme	chinensis	38:46	arg1	Polysaccharide					48:61	Schisandra chinensis Polysaccharide	27:61	Schisandra chinensis Polysaccharide	27:61	The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota.					
33194780	10	94	theme	inflammatory	1509:1520	arg1	factors					1522:1528	inflammatory factors	1509:1528	inflammatory factors	1509:1528	Results: The results showed that SCP could significantly improve the physiological state of UC mice and regulate the level of inflammatory factors to normal levels.					
33194780	12	95	theme	valeric	1782:1788	arg1	acid					1790:1793	valeric acid	1782:1793	valeric acid	1782:1793	In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota.					
33194780	10	96	theme	factors	1522:1528	arg1	level					1500:1504	the level	1496:1504	the level of inflammatory factors to normal levels	1496:1545	Results: The results showed that SCP could significantly improve the physiological state of UC mice and regulate the level of inflammatory factors to normal levels.					
33194780	7	97	theme	SCP	1037:1039	arg1	effect					1027:1032	the effect	1023:1032	the effect of SCP on the composition and diversity of gut microbiota	1023:1090	Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota.					
33194780	1	98	theme	factors	299:305	arg1	association					241:251	an intricate association	228:251	an intricate association of environmental, microbial, and host-related factors	228:305	Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors.					
33194780	7	99	from	effect	1027:1032	arg1	diversity					1064:1072	diversity	1064:1072	diversity	1064:1072	Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota.					
33194780	7	99	from	effect	1027:1032	arg1	composition					1048:1058	composition	1048:1058	composition	1048:1058	Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota.					
33194780	3	100	theme	Schisandra	494:503	arg1	polysaccharide					515:528	Schisandra chinensis polysaccharide	494:528	Schisandra chinensis polysaccharide (SCP)	494:534	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	3	100	theme	Schisandra	494:503	arg1	SCP					531:533	SCP	531:533	SCP	531:533	However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied.					
33194780	9	101	theme	short	1250:1254	arg1	acids					1268:1272	short chain fatty acids	1250:1272	short chain fatty acids	1250:1272	Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC.					
33194780	13	102	theme	inflammatory	1913:1924	arg1	disease					1932:1938	inflammatory bowel disease	1913:1938	inflammatory bowel disease	1913:1938	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
33194780	9	103	theme	fatty	1262:1266	arg1	acids					1268:1272	short chain fatty acids	1250:1272	short chain fatty acids	1250:1272	Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC.					
33194780	13	104	dep	composition	1958:1968	arg1	the					1954:1956	the	1954:1956	the	1954:1956	Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.					
34389385	6	0	theme	Paecilomyces	1130:1141	arg1	TJJ1213					1151:1157	Paecilomyces cicadae TJJ1213	1130:1157	Paecilomyces cicadae TJJ1213	1130:1157	These results suggested that EPS from Paecilomyces cicadae TJJ1213 can be used as a functional food to improve health and prevent diseases by promoting gut health.					
34389385	1	1	theme	purified	251:258	arg1	EPS2					298:301	EPS2	298:301	EPS2	298:301	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	1	theme	purified	251:258	arg1	EPS1					289:292	EPS1	289:292	EPS1	289:292	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	1	theme	purified	251:258	arg1	fractions					278:286	two purified exopolysaccharide fractions	247:286	two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213	247:336	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	0	2	theme	cicadae	93:99	arg1	TJJ1213					101:107	Paecilomyces cicadae TJJ1213	80:107	Paecilomyces cicadae TJJ1213	80:107	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	3	from	digestion	10:18	arg1	microbiota					138:147	human gut microbiota	128:147	human gut microbiota	128:147	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	3	from	digestion	10:18	arg1	TJJ1213					101:107	Paecilomyces cicadae TJJ1213	80:107	Paecilomyces cicadae TJJ1213	80:107	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	1	4	from	TJJ1213	330:336	arg1	behaviors					234:242	fecal fermentation behaviors	215:242	fecal fermentation behaviors	215:242	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	4	from	TJJ1213	330:336	arg1	EPS1					289:292	EPS1	289:292	EPS1	289:292	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	4	from	TJJ1213	330:336	arg1	EPS2					298:301	EPS2	298:301	EPS2	298:301	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	4	from	TJJ1213	330:336	arg1	digestion					201:209	the in vitro simulated digestion	178:209	the in vitro simulated digestion	178:209	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	4	from	TJJ1213	330:336	arg1	effects					346:352	its effects	342:352	its effects on human gut microbiota composition	342:388	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	4	from	TJJ1213	330:336	arg1	fractions					278:286	two purified exopolysaccharide fractions	247:286	two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213	247:336	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	3	5	theme	molecular	514:522	arg1	weight					524:529	the molecular weight	510:529	the molecular weight of EPS1 and EPS2	510:546	After fecal fermentation, however, the molecular weight of EPS1 and EPS2 significantly decreased, and the molar ratios of the monosaccharide composition remarkably changed, indicating that EPS1 and EPS2 could be degraded and consumed by human gut microbiota.					
34389385	1	6	theme	exopolysaccharide	260:276	arg1	EPS2					298:301	EPS2	298:301	EPS2	298:301	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	6	theme	exopolysaccharide	260:276	arg1	EPS1					289:292	EPS1	289:292	EPS1	289:292	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	6	theme	exopolysaccharide	260:276	arg1	fractions					278:286	two purified exopolysaccharide fractions	247:286	two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213	247:336	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	4	7	theme	pathogenic	902:911	arg1	Fusobacterium					959:971	Fusobacterium	959:971	Fusobacterium	959:971	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	4	7	theme	pathogenic	902:911	arg1	bacteria					913:920	the pathogenic bacteria	898:920	the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium	898:971	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	4	7	theme	pathogenic	902:911	arg1	Klebsiella					944:953	Klebsiella	944:953	Klebsiella	944:953	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	4	7	theme	pathogenic	902:911	arg1	Escherichia-Shigella					922:941	Escherichia-Shigella	922:941	Escherichia-Shigella	922:941	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	3	8	theme	EPS2	543:546	arg1	weight					524:529	the molecular weight	510:529	the molecular weight of EPS1 and EPS2	510:546	After fecal fermentation, however, the molecular weight of EPS1 and EPS2 significantly decreased, and the molar ratios of the monosaccharide composition remarkably changed, indicating that EPS1 and EPS2 could be degraded and consumed by human gut microbiota.					
34389385	1	9	theme	fractions	278:286	arg1	effects					346:352	its effects	342:352	its effects on human gut microbiota composition	342:388	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	9	theme	fractions	278:286	arg1	digestion					201:209	the in vitro simulated digestion	178:209	the in vitro simulated digestion	178:209	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	9	theme	fractions	278:286	arg1	behaviors					234:242	fecal fermentation behaviors	215:242	fecal fermentation behaviors	215:242	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	10	theme	human	357:361	arg1	composition					378:388	human gut microbiota composition	357:388	human gut microbiota composition	357:388	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	11	theme	gut	363:365	arg1	composition					378:388	human gut microbiota composition	357:388	human gut microbiota composition	357:388	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	4	12	theme	Bacteroides	845:855	arg1	abundances					831:840	the relative abundances	818:840	the relative abundances of Bacteroides and Phascolarctobacterium	818:881	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	3	13	theme	EPS1	534:537	arg1	weight					524:529	the molecular weight	510:529	the molecular weight of EPS1 and EPS2	510:546	After fecal fermentation, however, the molecular weight of EPS1 and EPS2 significantly decreased, and the molar ratios of the monosaccharide composition remarkably changed, indicating that EPS1 and EPS2 could be degraded and consumed by human gut microbiota.					
34389385	4	14	dep	bacteria	913:920	arg1	Fusobacterium					959:971	Fusobacterium	959:971	Fusobacterium	959:971	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	4	14	dep	bacteria	913:920	arg1	bacteria					913:920	the pathogenic bacteria	898:920	the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium	898:971	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	4	14	dep	bacteria	913:920	arg1	Klebsiella					944:953	Klebsiella	944:953	Klebsiella	944:953	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	4	14	dep	bacteria	913:920	arg1	Escherichia-Shigella					922:941	Escherichia-Shigella	922:941	Escherichia-Shigella	922:941	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	0	15	from	behaviors	43:51	arg1	microbiota					138:147	human gut microbiota	128:147	human gut microbiota	128:147	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	15	from	behaviors	43:51	arg1	TJJ1213					101:107	Paecilomyces cicadae TJJ1213	80:107	Paecilomyces cicadae TJJ1213	80:107	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	3	16	theme	gut	718:720	arg1	microbiota					722:731	human gut microbiota	712:731	human gut microbiota	712:731	After fecal fermentation, however, the molecular weight of EPS1 and EPS2 significantly decreased, and the molar ratios of the monosaccharide composition remarkably changed, indicating that EPS1 and EPS2 could be degraded and consumed by human gut microbiota.					
34389385	4	17	theme	Phascolarctobacterium	861:881	arg1	abundances					831:840	the relative abundances	818:840	the relative abundances of Bacteroides and Phascolarctobacterium	818:881	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	1	18	theme	in	182:183	arg1	digestion					201:209	the in vitro simulated digestion	178:209	the in vitro simulated digestion	178:209	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	0	19	theme	Simulated	0:8	arg1	digestion					10:18	Simulated digestion	0:18	Simulated digestion	0:18	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	20	theme	fecal	24:28	arg1	behaviors					43:51	fecal fermentation behaviors	24:51	fecal fermentation behaviors	24:51	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	21	theme	gut	134:136	arg1	microbiota					138:147	human gut microbiota	128:147	human gut microbiota	128:147	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	6	22	theme	functional	1176:1185	arg1	food					1187:1190	a functional food	1174:1190	a functional food to improve health and prevent diseases by promoting gut health	1174:1253	These results suggested that EPS from Paecilomyces cicadae TJJ1213 can be used as a functional food to improve health and prevent diseases by promoting gut health.					
34389385	6	22	theme	functional	1176:1185	arg1	EPS					1121:1123	EPS	1121:1123	EPS from Paecilomyces cicadae TJJ1213	1121:1157	These results suggested that EPS from Paecilomyces cicadae TJJ1213 can be used as a functional food to improve health and prevent diseases by promoting gut health.					
34389385	5	23	theme	fatty	1050:1054	arg1	acids					1056:1060	short-chain fatty acids	1038:1060	short-chain fatty acids	1038:1060	In addition, EPS1 and EPS2 also could promote the production of short-chain fatty acids during fermentation for 24 h.					
34389385	1	24	theme	simulated	191:199	arg1	digestion					201:209	the in vitro simulated digestion	178:209	the in vitro simulated digestion	178:209	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	0	25	theme	human	128:132	arg1	microbiota					138:147	human gut microbiota	128:147	human gut microbiota	128:147	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	26	from	TJJ1213	101:107	arg1	behaviors					43:51	fecal fermentation behaviors	24:51	fecal fermentation behaviors	24:51	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	26	from	TJJ1213	101:107	arg1	effects					117:123	its effects	113:123	its effects on human gut microbiota	113:147	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	26	from	TJJ1213	101:107	arg1	digestion					10:18	Simulated digestion	0:18	Simulated digestion	0:18	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	26	from	TJJ1213	101:107	arg1	exopolysaccharides					56:73	exopolysaccharides	56:73	exopolysaccharides from Paecilomyces cicadae TJJ1213	56:107	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	3	27	theme	fecal	481:485	arg1	fermentation					487:498	fecal fermentation	481:498	fecal fermentation	481:498	After fecal fermentation, however, the molecular weight of EPS1 and EPS2 significantly decreased, and the molar ratios of the monosaccharide composition remarkably changed, indicating that EPS1 and EPS2 could be degraded and consumed by human gut microbiota.					
34389385	1	28	from	effects	346:352	arg1	composition					378:388	human gut microbiota composition	357:388	human gut microbiota composition	357:388	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	28	from	effects	346:352	arg1	TJJ1213					330:336	Paecilomyces cicadae TJJ1213	309:336	Paecilomyces cicadae TJJ1213	309:336	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	29	dep	in	182:183	arg1	vitro					185:189	vitro	185:189	vitro	185:189	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	0	30	theme	fermentation	30:41	arg1	behaviors					43:51	fecal fermentation behaviors	24:51	fecal fermentation behaviors	24:51	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	5	31	theme	acids	1056:1060	arg1	production					1024:1033	the production	1020:1033	the production of short-chain fatty acids	1020:1060	In addition, EPS1 and EPS2 also could promote the production of short-chain fatty acids during fermentation for 24 h.					
34389385	1	32	theme	fecal	215:219	arg1	behaviors					234:242	fecal fermentation behaviors	215:242	fecal fermentation behaviors	215:242	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	0	33	theme	exopolysaccharides	56:73	arg1	behaviors					43:51	fecal fermentation behaviors	24:51	fecal fermentation behaviors	24:51	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	33	theme	exopolysaccharides	56:73	arg1	effects					117:123	its effects	113:123	its effects on human gut microbiota	113:147	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	33	theme	exopolysaccharides	56:73	arg1	digestion					10:18	Simulated digestion	0:18	Simulated digestion	0:18	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	1	34	theme	Paecilomyces	309:320	arg1	TJJ1213					330:336	Paecilomyces cicadae TJJ1213	309:336	Paecilomyces cicadae TJJ1213	309:336	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	3	35	theme	molar	581:585	arg1	ratios					587:592	the molar ratios	577:592	the molar ratios of the monosaccharide composition	577:626	After fecal fermentation, however, the molecular weight of EPS1 and EPS2 significantly decreased, and the molar ratios of the monosaccharide composition remarkably changed, indicating that EPS1 and EPS2 could be degraded and consumed by human gut microbiota.					
34389385	1	36	theme	microbiota	367:376	arg1	composition					378:388	human gut microbiota composition	357:388	human gut microbiota composition	357:388	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	37	theme	cicadae	322:328	arg1	TJJ1213					330:336	Paecilomyces cicadae TJJ1213	309:336	Paecilomyces cicadae TJJ1213	309:336	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	38	dep	fractions	278:286	arg1	EPS2					298:301	EPS2	298:301	EPS2	298:301	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	38	dep	fractions	278:286	arg1	EPS1					289:292	EPS1	289:292	EPS1	289:292	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	38	dep	fractions	278:286	arg1	fractions					278:286	two purified exopolysaccharide fractions	247:286	two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213	247:336	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	4	39	theme	relative	822:829	arg1	abundances					831:840	the relative abundances	818:840	the relative abundances of Bacteroides and Phascolarctobacterium	818:881	Notably, EPS1 and EPS2 could significantly modulate the composition, via increasing the relative abundances of Bacteroides and Phascolarctobacterium and decreasing the pathogenic bacteria Escherichia-Shigella, Klebsiella and Fusobacterium.					
34389385	5	40	theme	short-chain	1038:1048	arg1	acids					1056:1060	short-chain fatty acids	1038:1060	short-chain fatty acids	1038:1060	In addition, EPS1 and EPS2 also could promote the production of short-chain fatty acids during fermentation for 24 h.					
34389385	0	41	from	effects	117:123	arg1	microbiota					138:147	human gut microbiota	128:147	human gut microbiota	128:147	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	0	41	from	effects	117:123	arg1	TJJ1213					101:107	Paecilomyces cicadae TJJ1213	80:107	Paecilomyces cicadae TJJ1213	80:107	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	6	42	theme	gut	1244:1246	arg1	health					1248:1253	gut health	1244:1253	gut health	1244:1253	These results suggested that EPS from Paecilomyces cicadae TJJ1213 can be used as a functional food to improve health and prevent diseases by promoting gut health.					
34389385	1	43	from	digestion	201:209	arg1	composition					378:388	human gut microbiota composition	357:388	human gut microbiota composition	357:388	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	43	from	digestion	201:209	arg1	TJJ1213					330:336	Paecilomyces cicadae TJJ1213	309:336	Paecilomyces cicadae TJJ1213	309:336	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	6	44	from	TJJ1213	1151:1157	arg1	food					1187:1190	a functional food	1174:1190	a functional food to improve health and prevent diseases by promoting gut health	1174:1253	These results suggested that EPS from Paecilomyces cicadae TJJ1213 can be used as a functional food to improve health and prevent diseases by promoting gut health.					
34389385	6	44	from	TJJ1213	1151:1157	arg1	EPS					1121:1123	EPS	1121:1123	EPS from Paecilomyces cicadae TJJ1213	1121:1157	These results suggested that EPS from Paecilomyces cicadae TJJ1213 can be used as a functional food to improve health and prevent diseases by promoting gut health.					
34389385	1	45	theme	fermentation	221:232	arg1	behaviors					234:242	fecal fermentation behaviors	215:242	fecal fermentation behaviors	215:242	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	46	from	behaviors	234:242	arg1	composition					378:388	human gut microbiota composition	357:388	human gut microbiota composition	357:388	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	1	46	from	behaviors	234:242	arg1	TJJ1213					330:336	Paecilomyces cicadae TJJ1213	309:336	Paecilomyces cicadae TJJ1213	309:336	This study aimed to explore the in vitro simulated digestion and fecal fermentation behaviors of two purified exopolysaccharide fractions (EPS1 and EPS2) from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota composition.					
34389385	0	47	theme	Paecilomyces	80:91	arg1	TJJ1213					101:107	Paecilomyces cicadae TJJ1213	80:107	Paecilomyces cicadae TJJ1213	80:107	Simulated digestion and fecal fermentation behaviors of exopolysaccharides from Paecilomyces cicadae TJJ1213 and its effects on human gut microbiota.					
34389385	3	48	theme	monosaccharide	601:614	arg1	composition					616:626	the monosaccharide composition	597:626	the monosaccharide composition	597:626	After fecal fermentation, however, the molecular weight of EPS1 and EPS2 significantly decreased, and the molar ratios of the monosaccharide composition remarkably changed, indicating that EPS1 and EPS2 could be degraded and consumed by human gut microbiota.					
34389385	3	49	theme	composition	616:626	arg1	ratios					587:592	the molar ratios	577:592	the molar ratios of the monosaccharide composition	577:626	After fecal fermentation, however, the molecular weight of EPS1 and EPS2 significantly decreased, and the molar ratios of the monosaccharide composition remarkably changed, indicating that EPS1 and EPS2 could be degraded and consumed by human gut microbiota.					
34389385	6	50	used	used	1166:1169	arg2	EPS					1121:1123	EPS	1121:1123	EPS from Paecilomyces cicadae TJJ1213	1121:1157	These results suggested that EPS from Paecilomyces cicadae TJJ1213 can be used as a functional food to improve health and prevent diseases by promoting gut health.					
34389385	6	50	used	used	1166:1169	arg2	food					1187:1190	a functional food	1174:1190	a functional food to improve health and prevent diseases by promoting gut health	1174:1253	These results suggested that EPS from Paecilomyces cicadae TJJ1213 can be used as a functional food to improve health and prevent diseases by promoting gut health.					
34389385	6	51	theme	cicadae	1143:1149	arg1	TJJ1213					1151:1157	Paecilomyces cicadae TJJ1213	1130:1157	Paecilomyces cicadae TJJ1213	1130:1157	These results suggested that EPS from Paecilomyces cicadae TJJ1213 can be used as a functional food to improve health and prevent diseases by promoting gut health.					
34389385	3	52	theme	human	712:716	arg1	microbiota					722:731	human gut microbiota	712:731	human gut microbiota	712:731	After fecal fermentation, however, the molecular weight of EPS1 and EPS2 significantly decreased, and the molar ratios of the monosaccharide composition remarkably changed, indicating that EPS1 and EPS2 could be degraded and consumed by human gut microbiota.					
32554270	0	0	theme	natural	69:75	arg1	coagulant					77:85	natural coagulant	69:85	natural coagulant	69:85	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.					
32554270	4	1	theme	composite	518:526	arg1	design					528:533	Central composite design	510:533	Central composite design in response surface methodology	510:565	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	7	2	theme	hydrogen	1066:1073	arg1	bonding					1075:1081	hydrogen bonding	1066:1081	hydrogen bonding between guar and pollutant particles	1066:1118	Structural characterization using Fourier Transform Infrared analysis demonstrated that hydrogen bonding between guar and pollutant particles was involved in coagulation and flocculation process.					
32554270	2	3	theme	due	366:368	arg1	pollution					356:364	secondary environmental pollution	332:364	secondary environmental pollution due to residual inorganic coagulant in treated effluent	332:420	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	2	4	from	step	294:297	arg1	operation					311:319	landfill operation	302:319	landfill operation	302:319	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	4	5	theme	Chemical	607:614	arg1	removal					636:642	Chemical Oxygen Demand (COD) removal	607:642	Chemical Oxygen Demand (COD) removal	607:642	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	8	6	theme	guar	1185:1188	arg1	gum					1190:1192	guar gum coagulant	1185:1202	guar gum coagulant	1185:1202	Therefore, guar gum coagulant present potential to be an alternative in leachate treatment where pH requirement is not required during treatment.					
32554270	4	7	theme	response	538:545	arg1	methodology					555:565	response surface methodology	538:565	response surface methodology	538:565	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	1	8	from	application	164:174	arg1	approach					213:220	the approach	209:220	the approach	209:220	Sustainable wastewater treatment necessitates the application of natural and green material in the approach.					
32554270	7	9	theme	flocculation	1152:1163	arg1	process					1165:1171	coagulation and flocculation process	1136:1171	process	1165:1171	Structural characterization using Fourier Transform Infrared analysis demonstrated that hydrogen bonding between guar and pollutant particles was involved in coagulation and flocculation process.					
32554270	5	10	theme	44.39 mg/L	734:743	arg1	dosage					724:729	guar gum dosage	715:729	guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm	715:804	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	6	11	theme	guar	914:917	arg1	gum					919:921	guar gum	914:921	guar gum	914:921	Scanning electron microscopy showed that floc was compact and energy-dispersive-x-ray analysis showed that guar gum was capable to adsorb multiple ions from the leachate.					
32554270	4	12	from	design	528:533	arg1	methodology					555:565	response surface methodology	538:565	response surface methodology	538:565	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	2	13	theme	crucial	286:292	arg1	coagulant					249:257	a natural coagulant	239:257	selecting a natural coagulant in leachate treatment	229:279	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	2	13	theme	crucial	286:292	arg1	step					294:297	a crucial step	284:297	a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent	284:420	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	4	14	theme	Demand	623:628	arg1	removal					636:642	Chemical Oxygen Demand (COD) removal	607:642	Chemical Oxygen Demand (COD) removal	607:642	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	8	15	theme	coagulant	1194:1202	arg1	gum					1190:1192	guar gum coagulant	1185:1202	guar gum coagulant	1185:1202	Therefore, guar gum coagulant present potential to be an alternative in leachate treatment where pH requirement is not required during treatment.					
32554270	5	16	theme	COD	693:695	arg1	%					710:710	the optimum COD removal 22.57%	681:710	the optimum COD removal 22.57%	681:710	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	7	17	dep	Transform	1020:1028	arg1	Infrared					1030:1037	Infrared	1030:1037	Transform Infrared analysis	1020:1046	Structural characterization using Fourier Transform Infrared analysis demonstrated that hydrogen bonding between guar and pollutant particles was involved in coagulation and flocculation process.					
32554270	4	18	theme	Oxygen	616:621	arg1	COD					631:633	COD	631:633	COD	631:633	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	4	18	theme	Oxygen	616:621	arg1	Demand					623:628	Oxygen Demand	616:628	Chemical Oxygen Demand (COD) removal	607:642	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	5	19	theme	pH	746:747	arg1	dosage					724:729	guar gum dosage	715:729	guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm	715:804	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	1	20	theme	natural	179:185	arg1	material					197:204	natural and green material	179:204	natural and green material	179:204	Sustainable wastewater treatment necessitates the application of natural and green material in the approach.					
32554270	0	21	theme	floc	91:94	arg1	characterization					96:111	floc characterization	91:111	floc characterization	91:111	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.					
32554270	4	22	theme	Central	510:516	arg1	design					528:533	Central composite design	510:533	Central composite design in response surface methodology	510:565	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	4	23	used	used	571:574	arg2	design					528:533	Central composite design	510:533	Central composite design in response surface methodology	510:565	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	5	24	theme	79.27 rpm	796:804	arg1	dosage					724:729	guar gum dosage	715:729	guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm	715:804	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	0	25	theme	landfill	12:19	arg1	leachate					21:28	Sustainable landfill leachate	0:28	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.	0:112	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.					
32554270	6	26	theme	energy-dispersive-x-ray	869:891	arg1	analysis					893:900	energy-dispersive-x-ray analysis	869:900	energy-dispersive-x-ray analysis	869:900	Scanning electron microscopy showed that floc was compact and energy-dispersive-x-ray analysis showed that guar gum was capable to adsorb multiple ions from the leachate.					
32554270	7	27	dep	guar	1091:1094	arg1	particles					1110:1118	particles	1110:1118	particles	1110:1118	Structural characterization using Fourier Transform Infrared analysis demonstrated that hydrogen bonding between guar and pollutant particles was involved in coagulation and flocculation process.					
32554270	1	28	theme	green	191:195	arg1	material					197:204	natural and green material	179:204	natural and green material	179:204	Sustainable wastewater treatment necessitates the application of natural and green material in the approach.					
32554270	0	29	theme	Sustainable	0:10	arg1	leachate					21:28	Sustainable landfill leachate	0:28	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.	0:112	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.					
32554270	9	30	theme	removal	1382:1388	arg1	advantage					1390:1398	added pollutant removal advantage	1366:1398	added pollutant removal advantage	1366:1398	Simultaneously, adsorption by guar gum offers added pollutant removal advantage.					
32554270	9	31	theme	pollutant	1372:1380	arg1	advantage					1390:1398	added pollutant removal advantage	1366:1398	added pollutant removal advantage	1366:1398	Simultaneously, adsorption by guar gum offers added pollutant removal advantage.					
32554270	2	32	theme	landfill	302:309	arg1	operation					311:319	landfill operation	302:319	landfill operation	302:319	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	2	33	from	coagulant	249:257	arg1	treatment					271:279	leachate treatment	262:279	leachate treatment	262:279	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	2	34	theme	inorganic	382:390	arg1	coagulant					392:400	residual inorganic coagulant	373:400	residual inorganic coagulant in treated effluent	373:420	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	5	35	theme	guar	715:718	arg1	gum					720:722	guar gum	715:722	guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm	715:804	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	6	36	theme	Scanning	807:814	arg1	microscopy					825:834	Scanning electron microscopy	807:834	Scanning electron microscopy	807:834	Scanning electron microscopy showed that floc was compact and energy-dispersive-x-ray analysis showed that guar gum was capable to adsorb multiple ions from the leachate.					
32554270	0	37	theme	leachate	21:28	arg1	treatment					30:38	Sustainable landfill leachate treatment	0:38	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.	0:112	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.					
32554270	5	38	theme	gum	720:722	arg1	dosage					724:729	guar gum dosage	715:729	guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm	715:804	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	7	39	dep	Fourier	1012:1018	arg1	Transform					1020:1028	Transform	1020:1028	Transform Infrared analysis	1020:1046	Structural characterization using Fourier Transform Infrared analysis demonstrated that hydrogen bonding between guar and pollutant particles was involved in coagulation and flocculation process.					
32554270	8	40	from	alternative	1231:1241	arg1	treatment					1255:1263	leachate treatment	1246:1263	leachate treatment where pH requirement is not required during treatment	1246:1317	Therefore, guar gum coagulant present potential to be an alternative in leachate treatment where pH requirement is not required during treatment.					
32554270	3	41	theme	landfill	481:488	arg1	leachate					490:497	landfill leachate	481:497	landfill leachate treatment	481:507	Current study investigated the application of guar gum in landfill leachate treatment.					
32554270	3	42	theme	Current	423:429	arg1	study					431:435	Current study	423:435	Current study	423:435	Current study investigated the application of guar gum in landfill leachate treatment.					
32554270	2	43	theme	secondary	332:340	arg1	pollution					356:364	secondary environmental pollution	332:364	secondary environmental pollution due to residual inorganic coagulant in treated effluent	332:420	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	1	44	theme	material	197:204	arg1	application					164:174	the application	160:174	the application of natural and green material in the approach	160:220	Sustainable wastewater treatment necessitates the application of natural and green material in the approach.					
32554270	3	45	theme	guar	469:472	arg1	gum					474:476	guar gum	469:476	guar gum	469:476	Current study investigated the application of guar gum in landfill leachate treatment.					
32554270	2	46	theme	treated	405:411	arg1	effluent					413:420	treated effluent	405:420	treated effluent	405:420	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	8	47	theme	leachate	1246:1253	arg1	treatment					1255:1263	leachate treatment	1246:1263	leachate treatment where pH requirement is not required during treatment	1246:1317	Therefore, guar gum coagulant present potential to be an alternative in leachate treatment where pH requirement is not required during treatment.					
32554270	2	48	theme	selecting	229:237	arg1	coagulant					249:257	a natural coagulant	239:257	selecting a natural coagulant in leachate treatment	229:279	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	2	48	theme	selecting	229:237	arg1	step					294:297	a crucial step	284:297	a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent	284:420	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	2	49	from	coagulant	392:400	arg1	effluent					413:420	treated effluent	405:420	treated effluent	405:420	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	3	50	theme	gum	474:476	arg1	application					454:464	the application	450:464	the application of guar gum in landfill leachate treatment	450:507	Current study investigated the application of guar gum in landfill leachate treatment.					
32554270	8	51	theme	pH	1271:1272	arg1	requirement					1274:1284	pH requirement	1271:1284	pH requirement	1271:1284	Therefore, guar gum coagulant present potential to be an alternative in leachate treatment where pH requirement is not required during treatment.					
32554270	4	52	theme	surface	547:553	arg1	methodology					555:565	response surface methodology	538:565	response surface methodology	538:565	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	5	53	theme	removal	697:703	arg1	%					710:710	the optimum COD removal 22.57%	681:710	the optimum COD removal 22.57%	681:710	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	5	54	theme	natural	755:761	arg1	pH					763:764	natural pH	755:764	natural pH of leachate	755:776	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	5	55	dep	pH	746:747	arg1	pH					763:764	natural pH	755:764	natural pH of leachate	755:776	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	2	56	theme	environmental	342:354	arg1	pollution					356:364	secondary environmental pollution	332:364	secondary environmental pollution due to residual inorganic coagulant in treated effluent	332:420	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	5	57	theme	mixing	783:788	arg1	79.27 rpm					796:804	mixing speed 79.27 rpm	783:804	mixing speed 79.27 rpm	783:804	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	9	58	theme	guar	1350:1353	arg1	gum					1355:1357	guar gum	1350:1357	guar gum	1350:1357	Simultaneously, adsorption by guar gum offers added pollutant removal advantage.					
32554270	5	59	theme	optimum	685:691	arg1	%					710:710	the optimum COD removal 22.57%	681:710	the optimum COD removal 22.57%	681:710	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	5	60	theme	Quadratic	645:653	arg1	model					655:659	Quadratic model	645:659	Quadratic model developed	645:669	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	1	61	theme	Sustainable	114:124	arg1	treatment					137:145	Sustainable wastewater treatment	114:145	Sustainable wastewater treatment	114:145	Sustainable wastewater treatment necessitates the application of natural and green material in the approach.					
32554270	9	62	theme	added	1366:1370	arg1	advantage					1390:1398	added pollutant removal advantage	1366:1398	added pollutant removal advantage	1366:1398	Simultaneously, adsorption by guar gum offers added pollutant removal advantage.					
32554270	0	63	dep	treatment	30:38	arg1	Optimize					41:48	Optimize	41:48	Optimize use of guar gum as natural coagulant and floc characterization	41:111	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.					
32554270	2	64	theme	leachate	262:269	arg1	treatment					271:279	leachate treatment	262:279	leachate treatment	262:279	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	1	65	theme	wastewater	126:135	arg1	treatment					137:145	Sustainable wastewater treatment	114:145	Sustainable wastewater treatment	114:145	Sustainable wastewater treatment necessitates the application of natural and green material in the approach.					
32554270	7	66	theme	Structural	978:987	arg1	characterization					989:1004	Structural characterization	978:1004	Structural characterization using Fourier Transform Infrared analysis	978:1046	Structural characterization using Fourier Transform Infrared analysis demonstrated that hydrogen bonding between guar and pollutant particles was involved in coagulation and flocculation process.					
32554270	5	67	theme	leachate	769:776	arg1	pH					763:764	natural pH	755:764	natural pH of leachate	755:776	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	7	68	theme	coagulation	1136:1146	arg1	process					1165:1171	coagulation and flocculation process	1136:1171	process	1165:1171	Structural characterization using Fourier Transform Infrared analysis demonstrated that hydrogen bonding between guar and pollutant particles was involved in coagulation and flocculation process.					
32554270	3	69	theme	leachate	490:497	arg1	treatment					499:507	landfill leachate treatment	481:507	landfill leachate treatment	481:507	Current study investigated the application of guar gum in landfill leachate treatment.					
32554270	0	70	theme	gum	62:64	arg1	use					50:52	use	50:52	use of guar gum	50:64	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.					
32554270	6	71	theme	electron	816:823	arg1	microscopy					825:834	Scanning electron microscopy	807:834	Scanning electron microscopy	807:834	Scanning electron microscopy showed that floc was compact and energy-dispersive-x-ray analysis showed that guar gum was capable to adsorb multiple ions from the leachate.					
32554270	2	72	theme	residual	373:380	arg1	coagulant					392:400	residual inorganic coagulant	373:400	residual inorganic coagulant in treated effluent	373:420	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	4	73	theme	removal	636:642	arg1	performance					592:602	the performance	588:602	the performance of Chemical Oxygen Demand (COD) removal	588:642	Central composite design in response surface methodology was used to optimize the performance of Chemical Oxygen Demand (COD) removal.					
32554270	0	74	theme	guar	57:60	arg1	gum					62:64	guar gum	57:64	guar gum	57:64	Sustainable landfill leachate treatment: Optimize use of guar gum as natural coagulant and floc characterization.					
32554270	2	75	theme	natural	241:247	arg1	coagulant					249:257	a natural coagulant	239:257	selecting a natural coagulant in leachate treatment	229:279	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	2	75	theme	natural	241:247	arg1	step					294:297	a crucial step	284:297	a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent	284:420	Thus, selecting a natural coagulant in leachate treatment is a crucial step in landfill operation to prevent secondary environmental pollution due to residual inorganic coagulant in treated effluent.					
32554270	3	76	from	application	454:464	arg1	treatment					499:507	landfill leachate treatment	481:507	landfill leachate treatment	481:507	Current study investigated the application of guar gum in landfill leachate treatment.					
32554270	5	77	theme	speed	790:794	arg1	79.27 rpm					796:804	mixing speed 79.27 rpm	783:804	mixing speed 79.27 rpm	783:804	Quadratic model developed indicated the optimum COD removal 22.57% at guar gum dosage of 44.39 mg/L, pH 8.56 (natural pH of leachate) and mixing speed 79.27 rpm.					
32554270	6	78	theme	multiple	945:952	arg1	ions					954:957	multiple ions	945:957	multiple ions	945:957	Scanning electron microscopy showed that floc was compact and energy-dispersive-x-ray analysis showed that guar gum was capable to adsorb multiple ions from the leachate.					
33254408	8	0	dep	showed	946:951	arg1	down-regulated					1034:1047	down-regulated	1034:1047	down-regulated the expression of pectin methylesterase related genes	1034:1101	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	8	0	dep	showed	946:951	arg1	up-regulated					956:967	up-regulated	956:967	up-regulated the expression level of lignin and cellulose synthase genes	956:1027	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	10	1	theme	gene	1279:1282	arg1	expression					1284:1293	related gene expression	1271:1293	related gene expression	1271:1293	These results indicated that SA pretreatment up-regulated cell wall polysaccharide synthesis and related gene expression to thicken the cell wall and block Cd from passing through.					
33254408	11	2	theme	Cd	1444:1445	arg1	accumulation					1447:1458	cell wall Cd accumulation	1434:1458	cell wall Cd accumulation	1434:1458	Furthermore, SA decreased pectin methylesterase activity and content to reduce cell wall Cd accumulation and change the Cd partition ratio.					
33254408	5	3	from	accumulation	632:643	arg1	plants					655:660	tomato plants	648:660	tomato plants	648:660	The results showed that exogenous SA significantly reduced Cd accumulation in tomato plants and changed Cd distribution.					
33254408	5	4	theme	Cd	629:630	arg1	accumulation					632:643	Cd accumulation	629:643	Cd accumulation in tomato plants	629:660	The results showed that exogenous SA significantly reduced Cd accumulation in tomato plants and changed Cd distribution.					
33254408	0	5	theme	Cd	104:105	arg1	accumulation					107:118	Cd accumulation	104:118	Cd accumulation of tomato	104:128	Exogenous salicylic acid regulates cell wall polysaccharides synthesis and pectin methylation to reduce Cd accumulation of tomato.					
33254408	6	6	theme	cell	708:711	arg1	composition					718:728	the cell wall composition	704:728	the cell wall composition	704:728	By analyzing the cell wall composition, it was found cellulose, hemicellulose, pectin, and lignin were induced by SA.					
33254408	4	7	used	used	484:487	arg2	seedlings					469:477	seedlings	469:477	seedlings	469:477	In this study, all tomato seedlings were pre-treated with 100 μM SA for 3 d, then seedlings were used to analyze the role of SA in regulating plant cell wall resistance to Cd stress.					
33254408	11	8	theme	methylesterase	1388:1401	arg1	activity					1403:1410	pectin methylesterase activity	1381:1410	pectin methylesterase activity	1381:1410	Furthermore, SA decreased pectin methylesterase activity and content to reduce cell wall Cd accumulation and change the Cd partition ratio.					
33254408	8	9	theme	expression	926:935	arg1	analysis					937:944	Gene expression analysis	921:944	Gene expression analysis	921:944	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	10	10	dep	wall	1315:1318	arg1	Cd					1330:1331	Cd	1330:1331	Cd	1330:1331	These results indicated that SA pretreatment up-regulated cell wall polysaccharide synthesis and related gene expression to thicken the cell wall and block Cd from passing through.					
33254408	3	11	theme	mediated	317:324	arg1	response					334:341	Salicylic acid (SA) mediated defense response	297:341	Salicylic acid (SA) mediated defense response	297:341	Salicylic acid (SA) mediated defense response increases plant resistance to heavy metals.					
33254408	8	12	theme	Gene	921:924	arg1	analysis					937:944	Gene expression analysis	921:944	Gene expression analysis	921:944	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	10	13	theme	cell	1310:1313	arg1	wall					1315:1318	the cell wall	1306:1318	the cell wall	1306:1318	These results indicated that SA pretreatment up-regulated cell wall polysaccharide synthesis and related gene expression to thicken the cell wall and block Cd from passing through.					
33254408	5	14	theme	Cd	674:675	arg1	distribution					677:688	Cd distribution	674:688	Cd distribution	674:688	The results showed that exogenous SA significantly reduced Cd accumulation in tomato plants and changed Cd distribution.					
33254408	10	15	theme	SA	1203:1204	arg1	pretreatment					1206:1217	SA pretreatment	1203:1217	SA pretreatment	1203:1217	These results indicated that SA pretreatment up-regulated cell wall polysaccharide synthesis and related gene expression to thicken the cell wall and block Cd from passing through.					
33254408	4	16	theme	SA	512:513	arg1	role					504:507	the role	500:507	the role of SA in regulating plant cell wall resistance to Cd stress	500:567	In this study, all tomato seedlings were pre-treated with 100 μM SA for 3 d, then seedlings were used to analyze the role of SA in regulating plant cell wall resistance to Cd stress.					
33254408	8	17	theme	pectin	1067:1072	arg1	genes					1097:1101	pectin methylesterase related genes	1067:1101	pectin methylesterase related genes	1067:1101	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	8	18	theme	genes	1023:1027	arg1	level					984:988	the expression level	969:988	the expression level of lignin and cellulose synthase genes	969:1027	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	2	19	theme	important	244:252	arg1	role					254:257	important role	244:257	important role	244:257	Plant cell wall plays important role in preventing Cd from entering cells.					
33254408	4	20	theme	100 μM	445:450	arg1	SA					452:453	100 μM SA	445:453	100 μM SA for 3 d	445:461	In this study, all tomato seedlings were pre-treated with 100 μM SA for 3 d, then seedlings were used to analyze the role of SA in regulating plant cell wall resistance to Cd stress.					
33254408	0	21	theme	wall	40:43	arg1	synthesis					61:69	cell wall polysaccharides synthesis	35:69	cell wall polysaccharides synthesis	35:69	Exogenous salicylic acid regulates cell wall polysaccharides synthesis and pectin methylation to reduce Cd accumulation of tomato.					
33254408	6	22	theme	wall	713:716	arg1	composition					718:728	the cell wall composition	704:728	the cell wall composition	704:728	By analyzing the cell wall composition, it was found cellulose, hemicellulose, pectin, and lignin were induced by SA.					
33254408	0	23	theme	Exogenous	0:8	arg1	acid					20:23	Exogenous salicylic acid	0:23	Exogenous salicylic acid	0:23	Exogenous salicylic acid regulates cell wall polysaccharides synthesis and pectin methylation to reduce Cd accumulation of tomato.					
33254408	1	24	theme	plant	158:162	arg1	growth					164:169	plant growth	158:169	plant growth	158:169	Cadmium (Cd) is harmful to plant growth and can be easily transferred from soil to plants.					
33254408	0	25	theme	salicylic	10:18	arg1	acid					20:23	Exogenous salicylic acid	0:23	Exogenous salicylic acid	0:23	Exogenous salicylic acid regulates cell wall polysaccharides synthesis and pectin methylation to reduce Cd accumulation of tomato.					
33254408	8	26	theme	methylesterase	1074:1087	arg1	genes					1097:1101	pectin methylesterase related genes	1067:1101	pectin methylesterase related genes	1067:1101	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	0	27	theme	pectin	75:80	arg1	methylation					82:92	pectin methylation	75:92	pectin methylation	75:92	Exogenous salicylic acid regulates cell wall polysaccharides synthesis and pectin methylation to reduce Cd accumulation of tomato.					
33254408	2	28	theme	entering	281:288	arg1	cells					290:294	entering cells	281:294	entering cells	281:294	Plant cell wall plays important role in preventing Cd from entering cells.					
33254408	8	29	theme	lignin	993:998	arg1	level					984:988	the expression level	969:988	the expression level of lignin and cellulose synthase genes	969:1027	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	3	30	theme	plant	353:357	arg1	resistance					359:368	plant resistance	353:368	plant resistance to heavy metals	353:384	Salicylic acid (SA) mediated defense response increases plant resistance to heavy metals.					
33254408	11	31	theme	Cd	1475:1476	arg1	ratio					1488:1492	the Cd partition ratio	1471:1492	the Cd partition ratio	1471:1492	Furthermore, SA decreased pectin methylesterase activity and content to reduce cell wall Cd accumulation and change the Cd partition ratio.					
33254408	10	32	theme	wall	1237:1240	arg1	synthesis					1257:1265	cell wall polysaccharide synthesis	1232:1265	cell wall polysaccharide synthesis	1232:1265	These results indicated that SA pretreatment up-regulated cell wall polysaccharide synthesis and related gene expression to thicken the cell wall and block Cd from passing through.					
33254408	3	33	theme	defense	326:332	arg1	response					334:341	Salicylic acid (SA) mediated defense response	297:341	Salicylic acid (SA) mediated defense response	297:341	Salicylic acid (SA) mediated defense response increases plant resistance to heavy metals.					
33254408	8	34	theme	expression	973:982	arg1	level					984:988	the expression level	969:988	the expression level of lignin and cellulose synthase genes	969:1027	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	11	35	theme	partition	1478:1486	arg1	ratio					1488:1492	the Cd partition ratio	1471:1492	the Cd partition ratio	1471:1492	Furthermore, SA decreased pectin methylesterase activity and content to reduce cell wall Cd accumulation and change the Cd partition ratio.					
33254408	10	36	theme	cell	1232:1235	arg1	synthesis					1257:1265	cell wall polysaccharide synthesis	1232:1265	cell wall polysaccharide synthesis	1232:1265	These results indicated that SA pretreatment up-regulated cell wall polysaccharide synthesis and related gene expression to thicken the cell wall and block Cd from passing through.					
33254408	8	37	theme	genes	1097:1101	arg1	expression					1053:1062	the expression	1049:1062	the expression of pectin methylesterase related genes	1049:1101	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	0	38	theme	tomato	123:128	arg1	accumulation					107:118	Cd accumulation	104:118	Cd accumulation of tomato	104:128	Exogenous salicylic acid regulates cell wall polysaccharides synthesis and pectin methylation to reduce Cd accumulation of tomato.					
33254408	11	39	theme	pectin	1381:1386	arg1	activity					1403:1410	pectin methylesterase activity	1381:1410	pectin methylesterase activity	1381:1410	Furthermore, SA decreased pectin methylesterase activity and content to reduce cell wall Cd accumulation and change the Cd partition ratio.					
33254408	3	40	theme	heavy	373:377	arg1	metals					379:384	heavy metals	373:384	heavy metals	373:384	Salicylic acid (SA) mediated defense response increases plant resistance to heavy metals.					
33254408	0	41	theme	cell	35:38	arg1	synthesis					61:69	cell wall polysaccharides synthesis	35:69	cell wall polysaccharides synthesis	35:69	Exogenous salicylic acid regulates cell wall polysaccharides synthesis and pectin methylation to reduce Cd accumulation of tomato.					
33254408	10	42	theme	related	1271:1277	arg1	expression					1284:1293	related gene expression	1271:1293	related gene expression	1271:1293	These results indicated that SA pretreatment up-regulated cell wall polysaccharide synthesis and related gene expression to thicken the cell wall and block Cd from passing through.					
33254408	4	43	theme	Cd	559:560	arg1	stress					562:567	Cd stress	559:567	Cd stress	559:567	In this study, all tomato seedlings were pre-treated with 100 μM SA for 3 d, then seedlings were used to analyze the role of SA in regulating plant cell wall resistance to Cd stress.					
33254408	5	44	theme	tomato	648:653	arg1	plants					655:660	tomato plants	648:660	tomato plants	648:660	The results showed that exogenous SA significantly reduced Cd accumulation in tomato plants and changed Cd distribution.					
33254408	4	45	theme	tomato	406:411	arg1	seedlings					413:421	all tomato seedlings	402:421	all tomato seedlings	402:421	In this study, all tomato seedlings were pre-treated with 100 μM SA for 3 d, then seedlings were used to analyze the role of SA in regulating plant cell wall resistance to Cd stress.					
33254408	0	46	theme	polysaccharides	45:59	arg1	synthesis					61:69	cell wall polysaccharides synthesis	35:69	cell wall polysaccharides synthesis	35:69	Exogenous salicylic acid regulates cell wall polysaccharides synthesis and pectin methylation to reduce Cd accumulation of tomato.					
33254408	5	47	theme	exogenous	594:602	arg1	SA					604:605	exogenous SA	594:605	exogenous SA	594:605	The results showed that exogenous SA significantly reduced Cd accumulation in tomato plants and changed Cd distribution.					
33254408	9	48	theme	pectin	1151:1156	arg1	methylesterase					1158:1171	pectin methylesterase	1151:1171	pectin methylesterase	1151:1171	In addition, SA down-regulated the activity of pectin methylesterase.					
33254408	7	49	from	content	828:834	arg1	pectin					845:850	pectin	845:850	pectin	845:850	Interestingly, the content of Cd in pectin decreased by SA pretreatment, however it was increased in cellulose.					
33254408	8	50	theme	synthase	1014:1021	arg1	genes					1023:1027	cellulose synthase genes	1004:1027	cellulose synthase genes	1004:1027	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	9	51	theme	methylesterase	1158:1171	arg1	activity					1139:1146	the activity	1135:1146	the activity of pectin methylesterase	1135:1171	In addition, SA down-regulated the activity of pectin methylesterase.					
33254408	10	52	theme	polysaccharide	1242:1255	arg1	synthesis					1257:1265	cell wall polysaccharide synthesis	1232:1265	cell wall polysaccharide synthesis	1232:1265	These results indicated that SA pretreatment up-regulated cell wall polysaccharide synthesis and related gene expression to thicken the cell wall and block Cd from passing through.					
33254408	4	53	theme	plant	529:533	arg1	resistance					545:554	plant cell wall resistance	529:554	plant cell wall resistance to Cd stress	529:567	In this study, all tomato seedlings were pre-treated with 100 μM SA for 3 d, then seedlings were used to analyze the role of SA in regulating plant cell wall resistance to Cd stress.					
33254408	8	54	theme	cellulose	1004:1012	arg1	genes					1023:1027	cellulose synthase genes	1004:1027	cellulose synthase genes	1004:1027	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	2	55	theme	cell	228:231	arg1	wall					233:236	Plant cell wall	222:236	Plant cell wall	222:236	Plant cell wall plays important role in preventing Cd from entering cells.					
33254408	10	56	theme	thicken	1298:1304	arg1	wall					1315:1318	the cell wall	1306:1318	the cell wall	1306:1318	These results indicated that SA pretreatment up-regulated cell wall polysaccharide synthesis and related gene expression to thicken the cell wall and block Cd from passing through.					
33254408	7	57	theme	Cd	839:840	arg1	content					828:834	the content	824:834	the content of Cd in pectin	824:850	Interestingly, the content of Cd in pectin decreased by SA pretreatment, however it was increased in cellulose.					
33254408	3	58	theme	Salicylic	297:305	arg1	SA					313:314	SA	313:314	SA	313:314	Salicylic acid (SA) mediated defense response increases plant resistance to heavy metals.					
33254408	3	58	theme	Salicylic	297:305	arg1	acid					307:310	Salicylic acid	297:310	Salicylic acid (SA) mediated defense response	297:341	Salicylic acid (SA) mediated defense response increases plant resistance to heavy metals.					
33254408	11	59	theme	cell	1434:1437	arg1	accumulation					1447:1458	cell wall Cd accumulation	1434:1458	cell wall Cd accumulation	1434:1458	Furthermore, SA decreased pectin methylesterase activity and content to reduce cell wall Cd accumulation and change the Cd partition ratio.					
33254408	2	60	theme	Plant	222:226	arg1	wall					233:236	Plant cell wall	222:236	Plant cell wall	222:236	Plant cell wall plays important role in preventing Cd from entering cells.					
33254408	4	61	theme	wall	540:543	arg1	resistance					545:554	plant cell wall resistance	529:554	plant cell wall resistance to Cd stress	529:567	In this study, all tomato seedlings were pre-treated with 100 μM SA for 3 d, then seedlings were used to analyze the role of SA in regulating plant cell wall resistance to Cd stress.					
33254408	3	62	theme	acid	307:310	arg1	response					334:341	Salicylic acid (SA) mediated defense response	297:341	Salicylic acid (SA) mediated defense response	297:341	Salicylic acid (SA) mediated defense response increases plant resistance to heavy metals.					
33254408	7	63	theme	SA	865:866	arg1	pretreatment					868:879	SA pretreatment	865:879	SA pretreatment	865:879	Interestingly, the content of Cd in pectin decreased by SA pretreatment, however it was increased in cellulose.					
33254408	8	64	theme	related	1089:1095	arg1	genes					1097:1101	pectin methylesterase related genes	1067:1101	pectin methylesterase related genes	1067:1101	Gene expression analysis showed SA up-regulated the expression level of lignin and cellulose synthase genes, but down-regulated the expression of pectin methylesterase related genes.					
33254408	11	65	theme	wall	1439:1442	arg1	accumulation					1447:1458	cell wall Cd accumulation	1434:1458	cell wall Cd accumulation	1434:1458	Furthermore, SA decreased pectin methylesterase activity and content to reduce cell wall Cd accumulation and change the Cd partition ratio.					
33254408	4	66	theme	cell	535:538	arg1	resistance					545:554	plant cell wall resistance	529:554	plant cell wall resistance to Cd stress	529:567	In this study, all tomato seedlings were pre-treated with 100 μM SA for 3 d, then seedlings were used to analyze the role of SA in regulating plant cell wall resistance to Cd stress.					
35662980	7	0	gly	N-glycoproteins	1370:1384	arg1	N-glycoproteins					1370:1384	well-characterized therapeutic N-glycoproteins	1339:1384	well-characterized therapeutic N-glycoproteins	1339:1384	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	11	1	theme	peptide	2130:2136	arg1	sequence					2138:2145	the underlying peptide sequence	2115:2145	the underlying peptide sequence	2115:2145	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	3	2	theme	N-glycan	492:499	arg1	oligosaccharides					501:516	N-glycan oligosaccharides	492:516	N-glycan oligosaccharides	492:516	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	9	3	theme	high	1659:1662	arg1	mannose					1664:1670	high mannose	1659:1670	high mannose	1659:1670	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	11	4	theme	protein	1963:1969	arg1	structures					1979:1988	several protein crystal structures	1955:1988	several protein crystal structures	1955:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	4	5	theme	underlying	589:598	arg1	peptide					600:606	the underlying peptide	585:606	the underlying peptide	585:606	We found that the underlying peptide plays a role in fucosylation of paucimannose (low mannose) and high-mannose N-glycans but not for complex-type N-glycans.					
35662980	9	6	with	glycosites	1602:1611	arg1	N-glycans					1631:1639	complex-type N-glycans	1618:1639	complex-type N-glycans when converted to high mannose	1618:1670	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	8	7	gly	fucosylation	1412:1423	arg2	N-glycans					1455:1463	complex-type N-glycans	1442:1463	complex-type N-glycans	1442:1463	This confirmed that core fucosylation mainly occurs on complex-type N-glycans, although clearly only at selected glycosites.					
35662980	4	8	theme	low	654:656	arg1	mannose					658:664	paucimannose (low mannose) and high-mannose N-glycans	640:692	mannose	658:664	We found that the underlying peptide plays a role in fucosylation of paucimannose (low mannose) and high-mannose N-glycans but not for complex-type N-glycans.					
35662980	4	9	gly	fucosylation	624:635	arg1	N-glycans					684:692	paucimannose (low mannose) and high-mannose N-glycans	640:692	N-glycans	684:692	We found that the underlying peptide plays a role in fucosylation of paucimannose (low mannose) and high-mannose N-glycans but not for complex-type N-glycans.					
35662980	4	9	gly	fucosylation	624:635	arg1	mannose					658:664	paucimannose (low mannose) and high-mannose N-glycans	640:692	mannose	658:664	We found that the underlying peptide plays a role in fucosylation of paucimannose (low mannose) and high-mannose N-glycans but not for complex-type N-glycans.					
35662980	5	10	theme	G0	850:851	arg1	N-glycan					853:860	the G0 N-glycan	846:860	the G0 N-glycan	846:860	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	9	11	theme	core	1681:1684	arg1	Fuc					1686:1688	the core Fuc	1677:1688	the core Fuc	1677:1688	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	7	12	theme	therapeutic	1358:1368	arg1	N-glycoproteins					1370:1384	well-characterized therapeutic N-glycoproteins	1339:1384	well-characterized therapeutic N-glycoproteins	1339:1384	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	2	13	theme	complex	306:312	arg1	G0					340:341	G0	340:341	G0	340:341	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	2	13	theme	complex	306:312	arg1	oligosaccharide					323:337	the biantennary complex N-glycan oligosaccharide	290:337	the biantennary complex N-glycan oligosaccharide (G0)	290:342	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	5	14	theme	sugar	831:835	arg1	units					837:841	all sugar units	827:841	all sugar units of the G0 N-glycan	827:860	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	4	15	theme	paucimannose	640:651	arg1	mannose					658:664	paucimannose (low mannose) and high-mannose N-glycans	640:692	mannose	658:664	We found that the underlying peptide plays a role in fucosylation of paucimannose (low mannose) and high-mannose N-glycans but not for complex-type N-glycans.					
35662980	1	16	theme	core	222:225	arg1	fucosylation					227:238	core fucosylation	222:238	core fucosylation	222:238	FUT8 is an essential α-1,6-fucosyltransferase that fucosylates the innermost GlcNAc of N-glycans, a process called core fucosylation.					
35662980	11	17	theme	crystal	1971:1977	arg1	structures					1979:1988	several protein crystal structures	1955:1988	several protein crystal structures	1955:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	6	18	theme	N-glycans	1153:1161	arg1	recognition					1138:1148	an optimal recognition	1127:1148	an optimal recognition of N-glycans	1127:1161	The largest STD signals were observed in the presence of GDP, suggesting that prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc) is required for an optimal recognition of N-glycans.					
35662980	8	19	gly	glycosites	1500:1509	arg2	glycosites					1500:1509	selected glycosites	1491:1509	selected glycosites	1491:1509	This confirmed that core fucosylation mainly occurs on complex-type N-glycans, although clearly only at selected glycosites.					
35662980	3	20	link	Asn-linked	543:552	arg1	oligosaccharide					554:568	an Asn-linked oligosaccharide	540:568	an Asn-linked oligosaccharide	540:568	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	7	21	theme	N-glycans	1304:1312	arg1	fucosylation					1258:1269	FUT8 core fucosylation	1248:1269	FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins	1248:1384	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	4	22	theme	high-mannose	671:682	arg1	N-glycans					684:692	paucimannose (low mannose) and high-mannose N-glycans	640:692	N-glycans	684:692	We found that the underlying peptide plays a role in fucosylation of paucimannose (low mannose) and high-mannose N-glycans but not for complex-type N-glycans.					
35662980	7	23	theme	genetic	1175:1181	arg1	engineering					1183:1193	genetic engineering	1175:1193	genetic engineering of glycosylation capacities in CHO cells	1175:1234	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	11	24	theme	core	2005:2008	arg1	fucosylation					2010:2021	core fucosylation	2005:2021	core fucosylation	2005:2021	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	9	25	theme	Eliminating	1512:1522	arg1	capacity					1528:1535	the capacity	1524:1535	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1)	1512:1586	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	9	26	from	glycosylation	1554:1566	arg1	cells					1571:1575	cells	1571:1575	cells (KO mgat1)	1571:1586	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	9	26	from	glycosylation	1554:1566	arg1	mgat1					1581:1585	KO mgat1	1578:1585	KO mgat1	1578:1585	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	6	27	located	observed	1015:1022	arg1	presence					1031:1038	the presence	1027:1038	the presence of GDP	1027:1045	The largest STD signals were observed in the presence of GDP, suggesting that prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc) is required for an optimal recognition of N-glycans.					
35662980	6	27	located	observed	1015:1022	arg2	signals					1002:1008	The largest STD signals	986:1008	The largest STD signals	986:1008	The largest STD signals were observed in the presence of GDP, suggesting that prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc) is required for an optimal recognition of N-glycans.					
35662980	1	28	theme	innermost	174:182	arg1	GlcNAc					184:189	the innermost GlcNAc	170:189	the innermost GlcNAc of N-glycans	170:202	FUT8 is an essential α-1,6-fucosyltransferase that fucosylates the innermost GlcNAc of N-glycans, a process called core fucosylation.					
35662980	8	29	theme	selected	1491:1498	arg1	glycosites					1500:1509	selected glycosites	1491:1509	selected glycosites	1491:1509	This confirmed that core fucosylation mainly occurs on complex-type N-glycans, although clearly only at selected glycosites.					
35662980	0	30	theme	Protein	86:92	arg1	Environment					94:104	Protein Environment	86:104	Protein Environment	86:104	FUT8-Directed Core Fucosylation of N-glycans Is Regulated by the Glycan Structure and Protein Environment.					
35662980	5	31	theme	recognition	926:936	arg1	residues					889:896	the amino acid residues	874:896	the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST)	874:983	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	5	31	theme	recognition	926:936	arg1	sequon					938:943	a recognition sequon	924:943	a recognition sequon for the oligosaccharyltransferase (OST)	924:983	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	11	32	theme	several	1955:1961	arg1	structures					1979:1988	several protein crystal structures	1955:1988	several protein crystal structures	1955:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	5	33	theme	transfer	747:754	arg1	STD					768:770	STD	768:770	STD	768:770	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	5	33	theme	transfer	747:754	arg1	difference					756:765	saturation transfer difference	736:765	saturation transfer difference (STD) NMR spectroscopy	736:788	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	5	34	theme	amino	878:882	arg1	residues					889:896	the amino acid residues	874:896	the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST)	874:983	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	5	34	theme	amino	878:882	arg1	sequon					938:943	a recognition sequon	924:943	a recognition sequon for the oligosaccharyltransferase (OST)	924:983	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	5	34	theme	amino	878:882	arg1	Asn-X-Thr					899:907	Asn-X-Thr	899:907	Asn-X-Thr	899:907	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	7	35	theme	complex-type	1291:1302	arg1	N-glycans					1304:1312	high-mannose and complex-type N-glycans	1274:1312	N-glycans	1304:1312	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	12	36	theme	different	2281:2289	arg1	mechanisms					2291:2300	different mechanisms	2281:2300	different mechanisms for promoting core fucosylation	2281:2332	These data have further elucidated the different FUT8 acceptor substrate specificities both in vitro and in vivo in cells, revealing different mechanisms for promoting core fucosylation.					
35662980	0	37	theme	Glycan	65:70	arg1	Structure					72:80	Glycan Structure	65:80	Glycan Structure	65:80	FUT8-Directed Core Fucosylation of N-glycans Is Regulated by the Glycan Structure and Protein Environment.					
35662980	2	38	theme	protein/peptide	376:390	arg1	role					353:356	the role	349:356	the role of the underlying protein/peptide to which N-glycans are attached	349:422	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	0	39	theme	FUT8-Directed	0:12	arg1	Fucosylation					19:30	FUT8-Directed Core Fucosylation	0:30	FUT8-Directed Core Fucosylation of N-glycans	0:43	FUT8-Directed Core Fucosylation of N-glycans Is Regulated by the Glycan Structure and Protein Environment.					
35662980	0	40	dep	Structure	72:80	arg1	the					61:63	the	61:63	the	61:63	FUT8-Directed Core Fucosylation of N-glycans Is Regulated by the Glycan Structure and Protein Environment.					
35662980	7	41	theme	high-mannose	1274:1285	arg1	N-glycans					1304:1312	high-mannose and complex-type N-glycans	1274:1312	N-glycans	1304:1312	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	2	42	attach	attached	415:422	arg2	N-glycans					401:409	N-glycans	401:409	N-glycans	401:409	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	2	42	attach	attached	415:422	arg1	protein/peptide					376:390	the underlying protein/peptide	361:390	the underlying protein/peptide to which N-glycans are attached	361:422	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	9	43	gly	glycosylation	1554:1566	arg1	cells					1571:1575	cells	1571:1575	cells (KO mgat1)	1571:1586	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	9	43	gly	glycosylation	1554:1566	arg1	mgat1					1581:1585	KO mgat1	1578:1585	KO mgat1	1578:1585	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	12	44	theme	acceptor	2202:2209	arg1	specificities					2221:2233	the different FUT8 acceptor substrate specificities	2183:2233	the different FUT8 acceptor substrate specificities	2183:2233	These data have further elucidated the different FUT8 acceptor substrate specificities both in vitro and in vivo in cells, revealing different mechanisms for promoting core fucosylation.					
35662980	11	45	theme	N-glycosylation	1930:1944	arg1	structures					1979:1988	several protein crystal structures	1955:1988	several protein crystal structures	1955:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	11	45	theme	N-glycosylation	1930:1944	arg1	sites					1946:1950	the N-glycosylation sites	1926:1950	the N-glycosylation sites of several protein crystal structures	1926:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	0	46	theme	N-glycans	35:43	arg1	Fucosylation					19:30	FUT8-Directed Core Fucosylation	0:30	FUT8-Directed Core Fucosylation of N-glycans	0:43	FUT8-Directed Core Fucosylation of N-glycans Is Regulated by the Glycan Structure and Protein Environment.					
35662980	7	47	theme	FUT8	1248:1251	arg1	fucosylation					1258:1269	FUT8 core fucosylation	1248:1269	FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins	1248:1384	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	9	48	theme	complex-type	1618:1629	arg1	N-glycans					1631:1639	complex-type N-glycans	1618:1639	complex-type N-glycans when converted to high mannose	1618:1670	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	6	49	theme	prior	1064:1068	arg1	binding					1075:1081	prior FUT8 binding	1064:1081	prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc)	1064:1109	The largest STD signals were observed in the presence of GDP, suggesting that prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc) is required for an optimal recognition of N-glycans.					
35662980	6	50	theme	STD	998:1000	arg1	signals					1002:1008	The largest STD signals	986:1008	The largest STD signals	986:1008	The largest STD signals were observed in the presence of GDP, suggesting that prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc) is required for an optimal recognition of N-glycans.					
35662980	12	51	theme	different	2187:2195	arg1	specificities					2221:2233	the different FUT8 acceptor substrate specificities	2183:2233	the different FUT8 acceptor substrate specificities	2183:2233	These data have further elucidated the different FUT8 acceptor substrate specificities both in vitro and in vivo in cells, revealing different mechanisms for promoting core fucosylation.					
35662980	3	52	theme	oligosaccharides	501:516	arg1	oligosaccharide					554:568	an Asn-linked oligosaccharide	540:568	an Asn-linked oligosaccharide	540:568	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	3	52	theme	oligosaccharides	501:516	arg1	N-glycopeptides					519:533	N-glycopeptides	519:533	N-glycopeptides	519:533	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	3	52	theme	oligosaccharides	501:516	arg1	series					482:487	a series	480:487	a series of N-glycan oligosaccharides	480:516	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	7	53	theme	capacities	1212:1221	arg1	engineering					1183:1193	genetic engineering	1175:1193	genetic engineering of glycosylation capacities in CHO cells	1175:1234	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	10	54	from	Fuc	1873:1875	arg1	N-glycans					1897:1905	the high-mannose N-glycans	1880:1905	the high-mannose N-glycans	1880:1905	Interestingly, however, for erythropoietin that is uncommon among the tested glycoproteins in efficiently acquiring tetra-antennary N-glycans, two out of three N-glycosites obtained Fuc on the high-mannose N-glycans.					
35662980	11	55	theme	sequence	2138:2145	arg1	nature					2105:2110	the nature	2101:2110	the nature of the underlying peptide sequence	2101:2145	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	7	56	from	engineering	1183:1193	arg1	cells					1230:1234	CHO cells	1226:1234	CHO cells	1226:1234	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	6	57	theme	GDP	1043:1045	arg1	presence					1031:1038	the presence	1027:1038	the presence of GDP	1027:1045	The largest STD signals were observed in the presence of GDP, suggesting that prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc) is required for an optimal recognition of N-glycans.					
35662980	2	58	theme	biantennary	294:304	arg1	G0					340:341	G0	340:341	G0	340:341	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	2	58	theme	biantennary	294:304	arg1	oligosaccharide					323:337	the biantennary complex N-glycan oligosaccharide	290:337	the biantennary complex N-glycan oligosaccharide (G0)	290:342	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	11	59	theme	underlying	2119:2128	arg1	sequence					2138:2145	the underlying peptide sequence	2115:2145	the underlying peptide sequence	2115:2145	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	3	60	with	enzyme	468:473	arg1	oligosaccharide					554:568	an Asn-linked oligosaccharide	540:568	an Asn-linked oligosaccharide	540:568	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	3	60	with	enzyme	468:473	arg1	N-glycopeptides					519:533	N-glycopeptides	519:533	N-glycopeptides	519:533	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	3	60	with	enzyme	468:473	arg1	series					482:487	a series	480:487	a series of N-glycan oligosaccharides	480:516	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	5	61	theme	N-glycan	853:860	arg1	units					837:841	all sugar units	827:841	all sugar units of the G0 N-glycan	827:860	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	1	62	theme	N-glycans	194:202	arg1	GlcNAc					184:189	the innermost GlcNAc	170:189	the innermost GlcNAc of N-glycans	170:202	FUT8 is an essential α-1,6-fucosyltransferase that fucosylates the innermost GlcNAc of N-glycans, a process called core fucosylation.					
35662980	4	63	theme	mannose	658:664	arg1	fucosylation					624:635	fucosylation	624:635	fucosylation of paucimannose (low mannose) and high-mannose N-glycans	624:692	We found that the underlying peptide plays a role in fucosylation of paucimannose (low mannose) and high-mannose N-glycans but not for complex-type N-glycans.					
35662980	2	64	theme	N-glycan	314:321	arg1	G0					340:341	G0	340:341	G0	340:341	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	2	64	theme	N-glycan	314:321	arg1	oligosaccharide					323:337	the biantennary complex N-glycan oligosaccharide	290:337	the biantennary complex N-glycan oligosaccharide (G0)	290:342	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	7	65	theme	N-glycoproteins	1370:1384	arg1	panel					1330:1334	a panel	1328:1334	a panel of well-characterized therapeutic N-glycoproteins	1328:1384	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	11	66	theme	structures	1979:1988	arg1	structures					1979:1988	several protein crystal structures	1955:1988	several protein crystal structures	1955:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	11	66	theme	structures	1979:1988	arg1	sites					1946:1950	the N-glycosylation sites	1926:1950	the N-glycosylation sites of several protein crystal structures	1926:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	10	67	gly	N-glycosites	1851:1862	arg2	N-glycosites					1851:1862	two out of three N-glycosites	1834:1862	two out of three N-glycosites	1834:1862	Interestingly, however, for erythropoietin that is uncommon among the tested glycoproteins in efficiently acquiring tetra-antennary N-glycans, two out of three N-glycosites obtained Fuc on the high-mannose N-glycans.					
35662980	11	68	theme	N-glycan	2081:2088	arg1	accessibility					2049:2061	accessibility	2049:2061	accessibility	2049:2061	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	8	69	theme	complex-type	1442:1453	arg1	N-glycans					1455:1463	complex-type N-glycans	1442:1463	complex-type N-glycans	1442:1463	This confirmed that core fucosylation mainly occurs on complex-type N-glycans, although clearly only at selected glycosites.					
35662980	7	70	theme	well-characterized	1339:1356	arg1	N-glycoproteins					1370:1384	well-characterized therapeutic N-glycoproteins	1339:1384	well-characterized therapeutic N-glycoproteins	1339:1384	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	1	71	gly	fucosylates	158:168	arg1	GlcNAc					184:189	the innermost GlcNAc	170:189	the innermost GlcNAc of N-glycans	170:202	FUT8 is an essential α-1,6-fucosyltransferase that fucosylates the innermost GlcNAc of N-glycans, a process called core fucosylation.					
35662980	10	72	theme	tetra-antennary	1807:1821	arg1	N-glycans					1823:1831	tetra-antennary N-glycans	1807:1831	tetra-antennary N-glycans	1807:1831	Interestingly, however, for erythropoietin that is uncommon among the tested glycoproteins in efficiently acquiring tetra-antennary N-glycans, two out of three N-glycosites obtained Fuc on the high-mannose N-glycans.					
35662980	5	73	theme	acid	884:887	arg1	residues					889:896	the amino acid residues	874:896	the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST)	874:983	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	5	73	theme	acid	884:887	arg1	sequon					938:943	a recognition sequon	924:943	a recognition sequon for the oligosaccharyltransferase (OST)	924:983	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	5	73	theme	acid	884:887	arg1	Asn-X-Thr					899:907	Asn-X-Thr	899:907	Asn-X-Thr	899:907	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	11	74	gly	N-glycosylation	1930:1944	arg1	structures					1979:1988	several protein crystal structures	1955:1988	several protein crystal structures	1955:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	11	74	gly	N-glycosylation	1930:1944	arg2	sites					1946:1950	the N-glycosylation sites	1926:1950	the N-glycosylation sites of several protein crystal structures	1926:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	11	74	gly	N-glycosylation	1930:1944	arg2	structures					1979:1988	several protein crystal structures	1955:1988	several protein crystal structures	1955:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	2	75	theme	substrate	265:273	arg1	preference					275:284	substrate preference	265:284	substrate preference for the biantennary complex N-glycan oligosaccharide (G0)	265:342	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	1	76	theme	essential	118:126	arg1	process					207:213	a process	205:213	a process called core fucosylation	205:238	FUT8 is an essential α-1,6-fucosyltransferase that fucosylates the innermost GlcNAc of N-glycans, a process called core fucosylation.					
35662980	1	76	theme	essential	118:126	arg1	α-1,6-fucosyltransferase					128:151	an essential α-1,6-fucosyltransferase	115:151	an essential α-1,6-fucosyltransferase that fucosylates the innermost GlcNAc of N-glycans	115:202	FUT8 is an essential α-1,6-fucosyltransferase that fucosylates the innermost GlcNAc of N-glycans, a process called core fucosylation.					
35662980	1	76	theme	essential	118:126	arg1	FUT8					107:110	FUT8	107:110	FUT8	107:110	FUT8 is an essential α-1,6-fucosyltransferase that fucosylates the innermost GlcNAc of N-glycans, a process called core fucosylation.					
35662980	3	77	theme	Asn-linked	543:552	arg1	oligosaccharide					554:568	an Asn-linked oligosaccharide	540:568	an Asn-linked oligosaccharide	540:568	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	6	78	theme	largest	990:996	arg1	signals					1002:1008	The largest STD signals	986:1008	The largest STD signals	986:1008	The largest STD signals were observed in the presence of GDP, suggesting that prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc) is required for an optimal recognition of N-glycans.					
35662980	4	79	theme	complex-type	706:717	arg1	N-glycans					719:727	complex-type N-glycans	706:727	complex-type N-glycans	706:727	We found that the underlying peptide plays a role in fucosylation of paucimannose (low mannose) and high-mannose N-glycans but not for complex-type N-glycans.					
35662980	5	80	theme	difference	756:765	arg1	spectroscopy					777:788	saturation transfer difference (STD) NMR spectroscopy	736:788	saturation transfer difference (STD) NMR spectroscopy	736:788	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	11	81	dep	accessibility	2049:2061	arg1	the					2045:2047	the	2045:2047	the	2045:2047	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	6	82	theme	optimal	1130:1136	arg1	recognition					1138:1148	an optimal recognition	1127:1148	an optimal recognition of N-glycans	1127:1161	The largest STD signals were observed in the presence of GDP, suggesting that prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc) is required for an optimal recognition of N-glycans.					
35662980	7	83	with	cells	1317:1321	arg1	panel					1330:1334	a panel	1328:1334	a panel of well-characterized therapeutic N-glycoproteins	1328:1384	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	7	84	gly	fucosylation	1258:1269	arg1	cells					1317:1321	cells	1317:1321	cells with a panel of well-characterized therapeutic N-glycoproteins	1317:1384	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	7	84	gly	fucosylation	1258:1269	arg1	N-glycans					1304:1312	high-mannose and complex-type N-glycans	1274:1312	N-glycans	1304:1312	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	10	85	theme	high-mannose	1884:1895	arg1	N-glycans					1897:1905	the high-mannose N-glycans	1880:1905	the high-mannose N-glycans	1880:1905	Interestingly, however, for erythropoietin that is uncommon among the tested glycoproteins in efficiently acquiring tetra-antennary N-glycans, two out of three N-glycosites obtained Fuc on the high-mannose N-glycans.					
35662980	4	86	theme	N-glycans	684:692	arg1	fucosylation					624:635	fucosylation	624:635	fucosylation of paucimannose (low mannose) and high-mannose N-glycans	624:692	We found that the underlying peptide plays a role in fucosylation of paucimannose (low mannose) and high-mannose N-glycans but not for complex-type N-glycans.					
35662980	10	87	theme	tested	1761:1766	arg1	glycoproteins					1768:1780	the tested glycoproteins	1757:1780	the tested glycoproteins in efficiently acquiring tetra-antennary N-glycans	1757:1831	Interestingly, however, for erythropoietin that is uncommon among the tested glycoproteins in efficiently acquiring tetra-antennary N-glycans, two out of three N-glycosites obtained Fuc on the high-mannose N-glycans.					
35662980	9	88	theme	complex-type	1541:1552	arg1	glycosylation					1554:1566	complex-type glycosylation	1541:1566	complex-type glycosylation in cells (KO mgat1)	1541:1586	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	7	89	theme	CHO	1226:1228	arg1	cells					1230:1234	CHO cells	1226:1234	CHO cells	1226:1234	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	5	90	theme	saturation	736:745	arg1	STD					768:770	STD	768:770	STD	768:770	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	5	90	theme	saturation	736:745	arg1	difference					756:765	saturation transfer difference	736:765	saturation transfer difference (STD) NMR spectroscopy	736:788	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	0	91	theme	Core	14:17	arg1	Fucosylation					19:30	FUT8-Directed Core Fucosylation	0:30	FUT8-Directed Core Fucosylation of N-glycans	0:43	FUT8-Directed Core Fucosylation of N-glycans Is Regulated by the Glycan Structure and Protein Environment.					
35662980	2	92	theme	underlying	365:374	arg1	protein/peptide					376:390	the underlying protein/peptide	361:390	the underlying protein/peptide to which N-glycans are attached	361:422	In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear.					
35662980	7	93	theme	core	1253:1256	arg1	fucosylation					1258:1269	FUT8 core fucosylation	1248:1269	FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins	1248:1384	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	3	94	theme	FUT8	463:466	arg1	enzyme					468:473	the FUT8 enzyme	459:473	the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide	459:568	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	5	95	theme	NMR	773:775	arg1	spectroscopy					777:788	saturation transfer difference (STD) NMR spectroscopy	736:788	saturation transfer difference (STD) NMR spectroscopy	736:788	Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST).					
35662980	10	96	gly	glycoproteins	1768:1780	arg1	glycoproteins					1768:1780	the tested glycoproteins	1757:1780	the tested glycoproteins in efficiently acquiring tetra-antennary N-glycans	1757:1831	Interestingly, however, for erythropoietin that is uncommon among the tested glycoproteins in efficiently acquiring tetra-antennary N-glycans, two out of three N-glycosites obtained Fuc on the high-mannose N-glycans.					
35662980	12	97	theme	FUT8	2197:2200	arg1	specificities					2221:2233	the different FUT8 acceptor substrate specificities	2183:2233	the different FUT8 acceptor substrate specificities	2183:2233	These data have further elucidated the different FUT8 acceptor substrate specificities both in vitro and in vivo in cells, revealing different mechanisms for promoting core fucosylation.					
35662980	11	98	theme	sites	1946:1950	arg1	examination					1911:1921	An examination	1908:1921	An examination of the N-glycosylation sites of several protein crystal structures	1908:1988	An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence.					
35662980	0	99	gly	Fucosylation	19:30	arg1	N-glycans					35:43	N-glycans	35:43	N-glycans	35:43	FUT8-Directed Core Fucosylation of N-glycans Is Regulated by the Glycan Structure and Protein Environment.					
35662980	12	100	theme	substrate	2211:2219	arg1	specificities					2221:2233	the different FUT8 acceptor substrate specificities	2183:2233	the different FUT8 acceptor substrate specificities	2183:2233	These data have further elucidated the different FUT8 acceptor substrate specificities both in vitro and in vivo in cells, revealing different mechanisms for promoting core fucosylation.					
35662980	7	101	from	fucosylation	1258:1269	arg1	cells					1317:1321	cells	1317:1321	cells with a panel of well-characterized therapeutic N-glycoproteins	1317:1384	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	6	102	theme	FUT8	1070:1073	arg1	binding					1075:1081	prior FUT8 binding	1064:1081	prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc)	1064:1109	The largest STD signals were observed in the presence of GDP, suggesting that prior FUT8 binding to GDP-β-l-fucose (GDP-Fuc) is required for an optimal recognition of N-glycans.					
35662980	3	103	gly	N-glycopeptides	519:533	arg2	N-glycopeptides					519:533	N-glycopeptides	519:533	N-glycopeptides	519:533	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	3	103	gly	N-glycopeptides	519:533	arg1	oligosaccharides					501:516	N-glycan oligosaccharides	492:516	N-glycan oligosaccharides	492:516	Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide.					
35662980	12	104	theme	core	2316:2319	arg1	fucosylation					2321:2332	core fucosylation	2316:2332	core fucosylation	2316:2332	These data have further elucidated the different FUT8 acceptor substrate specificities both in vitro and in vivo in cells, revealing different mechanisms for promoting core fucosylation.					
35662980	7	105	theme	glycosylation	1198:1210	arg1	capacities					1212:1221	glycosylation capacities	1198:1221	glycosylation capacities	1198:1221	We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins.					
35662980	9	106	gly	glycosites	1602:1611	arg2	glycosites					1602:1611	glycosites	1602:1611	glycosites with complex-type N-glycans when converted to high mannose	1602:1670	Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc.					
35662980	8	107	theme	core	1407:1410	arg1	fucosylation					1412:1423	core fucosylation	1407:1423	core fucosylation	1407:1423	This confirmed that core fucosylation mainly occurs on complex-type N-glycans, although clearly only at selected glycosites.					
35662980	8	108	from	glycosites	1500:1509	arg1	only					1483:1486	only	1483:1486	only	1483:1486	This confirmed that core fucosylation mainly occurs on complex-type N-glycans, although clearly only at selected glycosites.					
32344089	7	0	theme	study	1098:1102	arg1	results					1071:1077	The results	1067:1077	The results of this systematic study	1067:1102	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	3	1	from	content	386:392	arg1	blends					401:406	the blends	397:406	the blends	397:406	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	7	2	theme	films	1241:1245	arg1	formation					1224:1232	the formation	1220:1232	the formation of the films with advanced mechanical and functional characteristics	1220:1301	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	1	3	theme	low	225:227	arg1	Chit					246:249	Chit	246:249	Chit	246:249	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	1	3	theme	low	225:227	arg1	chitosan					236:243	low weight chitosan	225:243	low weight chitosan (Chit)	225:250	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	5	4	from	increase	926:933	arg1	concentration					946:958	the PVA concentration	938:958	the PVA concentration	938:958	The mechanical characteristics of the composite films improved with an increase in the PVA concentration.					
32344089	7	5	with	films	1241:1245	arg1	characteristics					1287:1301	advanced mechanical and functional characteristics	1252:1301	advanced mechanical and functional characteristics	1252:1301	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	4	6	theme	films	575:579	arg1	results					538:544	The results	534:544	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods	534:645	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	1	7	theme	weight	229:234	arg1	Chit					246:249	Chit	246:249	Chit	246:249	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	1	7	theme	weight	229:234	arg1	chitosan					236:243	low weight chitosan	225:243	low weight chitosan (Chit)	225:250	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	3	8	from	70/30	517:521	arg1	fixed					508:512	fixed	508:512	fixed	508:512	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	3	8	from	70/30	517:521	arg1	ratio					498:502	the St/Chit ratio	486:502	the St/Chit ratio	486:502	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	4	9	theme	other	759:763	arg1	components					765:774	two other components	755:774	two other components of the blends	755:788	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	10	dep	analysis	581:588	arg1	methods					639:645	methods	639:645	methods	639:645	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	7	11	theme	mechanical	1261:1270	arg1	characteristics					1287:1301	advanced mechanical and functional characteristics	1252:1301	advanced mechanical and functional characteristics	1252:1301	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	0	12	theme	PVA	95:97	arg1	additions					99:107	PVA additions	95:107	PVA additions	95:107	Structure and properties of biodegradable maize starch/chitosan composite films as affected by PVA additions.					
32344089	4	13	dep	films	575:579	arg1	films					575:579	the St/Chit/PVA composite films	549:579	the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods	549:645	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	13	dep	films	575:579	arg1	analysis					581:588	analysis	581:588	analysis by X-ray diffraction	581:609	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	13	dep	films	575:579	arg1	spectroscopy					617:628	FTIR spectroscopy	612:628	FTIR spectroscopy	612:628	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	13	dep	films	575:579	arg1	SEM					635:637	SEM	635:637	SEM	635:637	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	3	14	theme	total	458:462	arg1	weight					464:469	the total weight	454:469	the total weight of the blend	454:482	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	3	15	from	weight	526:531	arg1	fixed					508:512	fixed	508:512	fixed	508:512	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	3	15	from	weight	526:531	arg1	ratio					498:502	the St/Chit ratio	486:502	the St/Chit ratio	486:502	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	6	16	theme	films	1026:1030	arg1	ability					979:985	UV-protective ability	965:985	UV-protective ability	965:985	The UV-protective ability and biodegradation rate of St/Chit/PVA films decreased as the PVA content grew.					
32344089	6	16	theme	films	1026:1030	arg1	rate					1006:1009	biodegradation rate	991:1009	biodegradation rate	991:1009	The UV-protective ability and biodegradation rate of St/Chit/PVA films decreased as the PVA content grew.					
32344089	4	17	theme	phase	722:726	arg1	separation					728:737	phase separation	722:737	phase separation between PVA and two other components of the blends	722:788	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	2	18	dep	features	309:316	arg1	the					294:296	the	294:296	the	294:296	To this end the structural features and functional characteristics of the films were evaluated.					
32344089	1	19	theme	composite	181:189	arg1	films					191:195	environmentally safe multi-purpose composite films	146:195	environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA)	146:279	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	1	20	theme	safe	162:165	arg1	films					191:195	environmentally safe multi-purpose composite films	146:195	environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA)	146:279	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	7	21	theme	biodegradability	1319:1334	arg1	loss					1311:1314	loss	1311:1314	loss of biodegradability	1311:1334	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	3	22	from	varied	412:417	arg1	range					426:430	the range	422:430	the range of 0-40 wt% (based on the total weight of the blend)	422:483	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	3	23	from	fixed	508:512	arg1	weight					526:531	weight	526:531	weight	526:531	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	1	24	theme	multi-purpose	167:179	arg1	films					191:195	environmentally safe multi-purpose composite films	146:195	environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA)	146:279	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	5	25	theme	films	903:907	arg1	characteristics					870:884	The mechanical characteristics	855:884	The mechanical characteristics of the composite films	855:907	The mechanical characteristics of the composite films improved with an increase in the PVA concentration.					
32344089	7	26	theme	St/Chit	1163:1169	arg1	matrix					1179:1184	the St/Chit (70/30) matrix	1159:1184	the St/Chit (70/30) matrix	1159:1184	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	7	27	theme	10-20 wt	1132:1139	arg1	additions					1146:1154	the 10-20 wt% PVA additions	1128:1154	the 10-20 wt% PVA additions to the St/Chit (70/30) matrix	1128:1184	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	7	27	theme	10-20 wt	1132:1139	arg1	supplement					1200:1209	an optimal supplement	1189:1209	an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability	1189:1334	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	6	28	theme	PVA	1049:1051	arg1	content					1053:1059	the PVA content	1045:1059	the PVA content	1045:1059	The UV-protective ability and biodegradation rate of St/Chit/PVA films decreased as the PVA content grew.					
32344089	2	29	theme	functional	322:331	arg1	characteristics					333:347	functional characteristics	322:347	functional characteristics	322:347	To this end the structural features and functional characteristics of the films were evaluated.					
32344089	3	30	theme	blend	478:482	arg1	weight					464:469	the total weight	454:469	the total weight of the blend	454:482	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	7	31	theme	functional	1276:1285	arg1	characteristics					1287:1301	advanced mechanical and functional characteristics	1252:1301	advanced mechanical and functional characteristics	1252:1301	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	3	32	from	range	426:430	arg1	varied					412:417	varied	412:417	varied	412:417	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	3	32	from	range	426:430	arg1	content					386:392	The PVA content	378:392	The PVA content in the blends	378:406	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	7	33	theme	advanced	1252:1259	arg1	characteristics					1287:1301	advanced mechanical and functional characteristics	1252:1301	advanced mechanical and functional characteristics	1252:1301	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	6	34	dep	ability	979:985	arg1	The					961:963	The	961:963	The	961:963	The UV-protective ability and biodegradation rate of St/Chit/PVA films decreased as the PVA content grew.					
32344089	4	35	theme	FTIR	612:615	arg1	films					575:579	the St/Chit/PVA composite films	549:579	the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods	549:645	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	35	theme	FTIR	612:615	arg1	spectroscopy					617:628	FTIR spectroscopy	612:628	FTIR spectroscopy	612:628	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	5	36	theme	PVA	942:944	arg1	concentration					946:958	the PVA concentration	938:958	the PVA concentration	938:958	The mechanical characteristics of the composite films improved with an increase in the PVA concentration.					
32344089	6	37	theme	UV-protective	965:977	arg1	ability					979:985	UV-protective ability	965:985	UV-protective ability	965:985	The UV-protective ability and biodegradation rate of St/Chit/PVA films decreased as the PVA content grew.					
32344089	4	38	theme	composite	565:573	arg1	films					575:579	the St/Chit/PVA composite films	549:579	the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods	549:645	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	38	theme	composite	565:573	arg1	analysis					581:588	analysis	581:588	analysis by X-ray diffraction	581:609	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	38	theme	composite	565:573	arg1	spectroscopy					617:628	FTIR spectroscopy	612:628	FTIR spectroscopy	612:628	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	38	theme	composite	565:573	arg1	SEM					635:637	SEM	635:637	SEM	635:637	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	0	39	theme	maize	42:46	arg1	films					74:78	biodegradable maize starch/chitosan composite films	28:78	biodegradable maize starch/chitosan composite films	28:78	Structure and properties of biodegradable maize starch/chitosan composite films as affected by PVA additions.					
32344089	4	40	theme	intermolecular	801:814	arg1	interactions					816:827	the intermolecular interactions	797:827	the intermolecular interactions between St and Chit	797:847	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	1	41	theme	maize	206:210	arg1	starch					212:217	maize starch	206:217	maize starch (St)	206:222	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	5	42	theme	composite	893:901	arg1	films					903:907	the composite films	889:907	the composite films	889:907	The mechanical characteristics of the composite films improved with an increase in the PVA concentration.					
32344089	2	43	theme	films	356:360	arg1	characteristics					333:347	functional characteristics	322:347	functional characteristics	322:347	To this end the structural features and functional characteristics of the films were evaluated.					
32344089	2	43	theme	films	356:360	arg1	features					309:316	structural features	298:316	structural features	298:316	To this end the structural features and functional characteristics of the films were evaluated.					
32344089	0	44	theme	biodegradable	28:40	arg1	films					74:78	biodegradable maize starch/chitosan composite films	28:78	biodegradable maize starch/chitosan composite films	28:78	Structure and properties of biodegradable maize starch/chitosan composite films as affected by PVA additions.					
32344089	1	45	theme	Polyvinyl	257:265	arg1	Alcohol					267:273	Polyvinyl Alcohol	257:273	Polyvinyl Alcohol (PVA)	257:279	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	1	45	theme	Polyvinyl	257:265	arg1	PVA					276:278	PVA	276:278	PVA	276:278	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	3	46	theme	St/Chit	490:496	arg1	fixed					508:512	fixed	508:512	fixed	508:512	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	3	46	theme	St/Chit	490:496	arg1	ratio					498:502	the St/Chit ratio	486:502	the St/Chit ratio	486:502	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	0	47	theme	composite	64:72	arg1	films					74:78	biodegradable maize starch/chitosan composite films	28:78	biodegradable maize starch/chitosan composite films	28:78	Structure and properties of biodegradable maize starch/chitosan composite films as affected by PVA additions.					
32344089	3	48	theme	PVA	382:384	arg1	varied					412:417	varied	412:417	varied	412:417	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	3	48	theme	PVA	382:384	arg1	content					386:392	The PVA content	378:392	The PVA content in the blends	378:406	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	6	49	theme	biodegradation	991:1004	arg1	rate					1006:1009	biodegradation rate	991:1009	biodegradation rate	991:1009	The UV-protective ability and biodegradation rate of St/Chit/PVA films decreased as the PVA content grew.					
32344089	4	50	theme	X-ray	593:597	arg1	diffraction					599:609	X-ray diffraction	593:609	X-ray diffraction	593:609	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	0	51	theme	starch/chitosan	48:62	arg1	films					74:78	biodegradable maize starch/chitosan composite films	28:78	biodegradable maize starch/chitosan composite films	28:78	Structure and properties of biodegradable maize starch/chitosan composite films as affected by PVA additions.					
32344089	6	52	theme	St/Chit/PVA	1014:1024	arg1	films					1026:1030	St/Chit/PVA films	1014:1030	St/Chit/PVA films	1014:1030	The UV-protective ability and biodegradation rate of St/Chit/PVA films decreased as the PVA content grew.					
32344089	7	53	theme	%	1140:1140	arg1	additions					1146:1154	the 10-20 wt% PVA additions	1128:1154	the 10-20 wt% PVA additions to the St/Chit (70/30) matrix	1128:1184	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	7	53	theme	%	1140:1140	arg1	supplement					1200:1209	an optimal supplement	1189:1209	an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability	1189:1334	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	4	54	theme	blends	783:788	arg1	components					765:774	two other components	755:774	two other components of the blends	755:788	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	54	theme	blends	783:788	arg1	PVA					747:749	PVA	747:749	PVA	747:749	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	5	55	theme	mechanical	859:868	arg1	characteristics					870:884	The mechanical characteristics	855:884	The mechanical characteristics of the composite films	855:907	The mechanical characteristics of the composite films improved with an increase in the PVA concentration.					
32344089	4	56	theme	PVA	663:665	arg1	concentration					667:679	the PVA concentration	659:679	the PVA concentration increase in the compositions	659:708	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	57	theme	St/Chit/PVA	553:563	arg1	films					575:579	the St/Chit/PVA composite films	549:579	the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods	549:645	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	57	theme	St/Chit/PVA	553:563	arg1	analysis					581:588	analysis	581:588	analysis by X-ray diffraction	581:609	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	57	theme	St/Chit/PVA	553:563	arg1	spectroscopy					617:628	FTIR spectroscopy	612:628	FTIR spectroscopy	612:628	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	4	57	theme	St/Chit/PVA	553:563	arg1	SEM					635:637	SEM	635:637	SEM	635:637	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	7	58	theme	PVA	1142:1144	arg1	additions					1146:1154	the 10-20 wt% PVA additions	1128:1154	the 10-20 wt% PVA additions to the St/Chit (70/30) matrix	1128:1184	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	7	58	theme	PVA	1142:1144	arg1	supplement					1200:1209	an optimal supplement	1189:1209	an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability	1189:1334	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	1	59	dep	starch	212:217	arg1	St					220:221	St	220:221	St	220:221	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	4	60	from	increase	681:688	arg1	compositions					697:708	the compositions	693:708	the compositions	693:708	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	1	61	theme	work	126:129	arg1	aim					114:116	The aim	110:116	The aim of this work	110:129	The aim of this work was to develop environmentally safe multi-purpose composite films based on maize starch (St), low weight chitosan (Chit), and Polyvinyl Alcohol (PVA).					
32344089	3	62	theme	%	442:442	arg1	range					426:430	the range	422:430	the range of 0-40 wt% (based on the total weight of the blend)	422:483	The PVA content in the blends was varied in the range of 0-40 wt% (based on the total weight of the blend), the St/Chit ratio was fixed at 70/30 in weight.					
32344089	7	63	theme	optimal	1192:1198	arg1	additions					1146:1154	the 10-20 wt% PVA additions	1128:1154	the 10-20 wt% PVA additions to the St/Chit (70/30) matrix	1128:1184	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	7	63	theme	optimal	1192:1198	arg1	supplement					1200:1209	an optimal supplement	1189:1209	an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability	1189:1334	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
32344089	0	64	theme	films	74:78	arg1	properties					14:23	properties	14:23	properties	14:23	Structure and properties of biodegradable maize starch/chitosan composite films as affected by PVA additions.					
32344089	0	64	theme	films	74:78	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and properties of biodegradable maize starch/chitosan composite films as affected by PVA additions.					
32344089	4	65	theme	concentration	667:679	arg1	increase					681:688	the PVA concentration increase	659:688	the PVA concentration increase in the compositions	659:708	The results of the St/Chit/PVA composite films analysis by X-ray diffraction, FTIR spectroscopy, and SEM methods showed that the PVA concentration increase in the compositions resulted in phase separation between PVA and two other components of the blends, while the intermolecular interactions between St and Chit grew.					
32344089	2	66	theme	structural	298:307	arg1	features					309:316	structural features	298:316	structural features	298:316	To this end the structural features and functional characteristics of the films were evaluated.					
32344089	7	67	theme	systematic	1087:1096	arg1	study					1098:1102	this systematic study	1082:1102	this systematic study	1082:1102	The results of this systematic study enabled us to recommend the 10-20 wt% PVA additions to the St/Chit (70/30) matrix as an optimal supplement ensuring the formation of the films with advanced mechanical and functional characteristics without loss of biodegradability.					
34091920	10	0	theme	development	1577:1587	arg1	exploration					1512:1522	further exploration	1504:1522	further exploration of its structure-activity relationships and activity development	1504:1587	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	4	1	theme	molecular	520:528	arg1	kDa					557:559	447.48 kDa	550:559	447.48 kDa	550:559	High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa.					
34091920	4	1	theme	molecular	520:528	arg1	weight					530:535	the molecular weight	516:535	the molecular weight of DOP-1	516:544	High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa.					
34091920	6	2	theme	spectroscopic	738:750	arg1	analysis					752:759	Methylation and nuclear magnetic resonance spectroscopic analysis	695:759	Methylation and nuclear magnetic resonance spectroscopic analysis	695:759	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	1	3	dep	BACKGROUND	110:119	arg1	purified					142:149	purified	142:149	purified in this study	142:163	BACKGROUND A polysaccharide was purified in this study, which was acquired from the fermentation broth of Dendrobium officinale Kimura et Migo.					
34091920	10	4	dep	CONCLUSION	1376:1385	arg1	support					1427:1433	support	1427:1433	support its favourable biological activities	1427:1470	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	10	4	dep	CONCLUSION	1376:1385	arg1	lay					1476:1478	lay	1476:1478	lay a strong foundation for further exploration of its structure-activity relationships and activity development	1476:1587	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	8	5	theme	metal	1293:1297	arg1	activity					1309:1316	good metal chelating activity	1288:1316	good metal chelating activity	1288:1316	DOP-1 demonstrated good scavenging activity in vitro toward 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals, as well as good metal chelating activity.					
34091920	4	6	theme	DOP-1	540:544	arg1	kDa					557:559	447.48 kDa	550:559	447.48 kDa	550:559	High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa.					
34091920	4	6	theme	DOP-1	540:544	arg1	weight					530:535	the molecular weight	516:535	the molecular weight of DOP-1	516:544	High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa.					
34091920	10	7	theme	strong	1482:1487	arg1	foundation					1489:1498	a strong foundation	1480:1498	a strong foundation for further exploration of its structure-activity relationships and activity development	1480:1587	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	6	8	theme	-α-d-Manp-	822:831	arg1	backbone					780:787	the backbone	776:787	the backbone of DOP-1	776:796	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	6	8	theme	-α-d-Manp-	822:831	arg1	-α-d-Manp-					858:867	→4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→	802:870	→4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→	802:870	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	5	9	from	present	610:616	arg1	DOP-1					621:625	DOP-1	621:625	DOP-1	621:625	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	10	10	theme	biological	1450:1459	arg1	activities					1461:1470	its favourable biological activities	1435:1470	its favourable biological activities	1435:1470	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	10	11	theme	structural	1391:1400	arg1	characteristics					1402:1416	The structural characteristics	1387:1416	The structural characteristics of DOP-1	1387:1425	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	3	12	theme	main	390:393	arg1	DOP-1					426:430	DOP-1	426:430	DOP-1	426:430	RESULTS The polysaccharide was purified and the main polysaccharide fraction (i.e., DOP-1) was obtained.					
34091920	3	12	theme	main	390:393	arg1	fraction					410:417	the main polysaccharide fraction	386:417	the main polysaccharide fraction (i.e., DOP-1)	386:431	RESULTS The polysaccharide was purified and the main polysaccharide fraction (i.e., DOP-1) was obtained.					
34091920	11	13	theme	Chemical	1712:1719	arg1	Industry					1721:1728	Chemical Industry	1712:1728	Chemical Industry	1712:1728	© 2021 Society of Chemical Industry.					
34091920	10	14	theme	favourable	1439:1448	arg1	activities					1461:1470	its favourable biological activities	1435:1470	its favourable biological activities	1435:1470	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	7	15	theme	In	934:935	arg1	tests					943:947	In vitro tests	934:947	In vitro tests	934:947	In vitro tests proved that DOP-1 not only protects RAW264.7 macrophages from the cytotoxic effect induced by lipopolysaccharide (LPS), but also inhibits cytokines (i.e., interleukin-6 and tumour necrosis factor-α) induced by LPS.					
34091920	7	16	dep	interleukin-6	1104:1116	arg1	i.e.					1098:1101	i.e.	1098:1101	i.e.	1098:1101	In vitro tests proved that DOP-1 not only protects RAW264.7 macrophages from the cytotoxic effect induced by lipopolysaccharide (LPS), but also inhibits cytokines (i.e., interleukin-6 and tumour necrosis factor-α) induced by LPS.					
34091920	6	17	theme	resonance	728:736	arg1	analysis					752:759	Methylation and nuclear magnetic resonance spectroscopic analysis	695:759	Methylation and nuclear magnetic resonance spectroscopic analysis	695:759	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	10	18	dep	development	1626:1636	arg1	the					1622:1624	the	1622:1624	the	1622:1624	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	6	19	theme	magnetic	719:726	arg1	resonance					728:736	Methylation and nuclear magnetic resonance	695:736	resonance	728:736	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	10	20	theme	fermentation	1657:1668	arg1	broth					1670:1674	fermentation broth	1657:1674	fermentation broth of D. officinale	1657:1691	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	3	21	dep	DOP-1	426:430	arg1	i.e.					420:423	i.e.	420:423	i.e.	420:423	RESULTS The polysaccharide was purified and the main polysaccharide fraction (i.e., DOP-1) was obtained.					
34091920	6	22	theme	-α-d-Glcp-	805:814	arg1	backbone					780:787	the backbone	776:787	the backbone of DOP-1	776:796	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	6	22	theme	-α-d-Glcp-	805:814	arg1	-α-d-Manp-					858:867	→4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→	802:870	→4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→	802:870	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	2	23	theme	structural	282:291	arg1	features					293:300	the structural features	278:300	the structural features	278:300	We aimed to investigate the structural features and bioactivity of this polysaccharide.					
34091920	6	24	theme	nuclear	711:717	arg1	resonance					728:736	Methylation and nuclear magnetic resonance	695:736	resonance	728:736	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	5	25	attach	present	610:616	arg2	mannose					585:591	mannose	585:591	mannose	585:591	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	5	25	attach	present	610:616	arg2	glucose					573:579	glucose	573:579	glucose	573:579	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	5	25	attach	present	610:616	arg1	DOP-1					621:625	DOP-1	621:625	DOP-1	621:625	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	5	25	attach	present	610:616	arg2	Galactose					562:570	Galactose	562:570	Galactose	562:570	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	5	25	attach	present	610:616	arg1	ratio					673:677	a ratio	671:677	a ratio of 1:1.79:6.71	671:692	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	10	26	theme	activity	1568:1575	arg1	development					1577:1587	activity development	1568:1587	activity development	1568:1587	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	5	27	theme	composition	646:656	arg1	analysis					658:665	monosaccharide composition analysis	631:665	monosaccharide composition analysis	631:665	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	6	28	theme	repeating	881:889	arg1	units					891:895	its repeating units	877:895	its repeating units	877:895	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	10	29	theme	relationships	1550:1562	arg1	exploration					1512:1522	further exploration	1504:1522	further exploration of its structure-activity relationships and activity development	1504:1587	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	1	30	theme	fermentation	194:205	arg1	broth					207:211	the fermentation broth	190:211	the fermentation broth of Dendrobium officinale Kimura et Migo	190:251	BACKGROUND A polysaccharide was purified in this study, which was acquired from the fermentation broth of Dendrobium officinale Kimura et Migo.					
34091920	0	31	theme	Structural	0:9	arg1	characterisation					11:26	Structural characterisation	0:26	Structural characterisation	0:26	Structural characterisation and bioactivity of polysaccharides isolated from fermented Dendrobium officinale.					
34091920	10	32	theme	DOP-1	1421:1425	arg1	characteristics					1402:1416	The structural characteristics	1387:1416	The structural characteristics of DOP-1	1387:1425	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	2	33	theme	polysaccharide	326:339	arg1	bioactivity					306:316	bioactivity	306:316	bioactivity of this polysaccharide	306:339	We aimed to investigate the structural features and bioactivity of this polysaccharide.					
34091920	2	33	theme	polysaccharide	326:339	arg1	features					293:300	the structural features	278:300	the structural features	278:300	We aimed to investigate the structural features and bioactivity of this polysaccharide.					
34091920	6	34	theme	Methylation	695:705	arg1	resonance					728:736	Methylation and nuclear magnetic resonance	695:736	resonance	728:736	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	10	35	theme	officinale	1682:1691	arg1	broth					1670:1674	fermentation broth	1657:1674	fermentation broth of D. officinale	1657:1691	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	3	36	theme	polysaccharide	395:408	arg1	DOP-1					426:430	DOP-1	426:430	DOP-1	426:430	RESULTS The polysaccharide was purified and the main polysaccharide fraction (i.e., DOP-1) was obtained.					
34091920	3	36	theme	polysaccharide	395:408	arg1	fraction					410:417	the main polysaccharide fraction	386:417	the main polysaccharide fraction (i.e., DOP-1)	386:431	RESULTS The polysaccharide was purified and the main polysaccharide fraction (i.e., DOP-1) was obtained.					
34091920	8	37	theme	good	1183:1186	arg1	activity					1199:1206	good scavenging activity	1183:1206	good scavenging activity	1183:1206	DOP-1 demonstrated good scavenging activity in vitro toward 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals, as well as good metal chelating activity.					
34091920	8	38	theme	hydroxyl	1258:1265	arg1	radicals					1267:1274	hydroxyl radicals	1258:1274	hydroxyl radicals	1258:1274	DOP-1 demonstrated good scavenging activity in vitro toward 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals, as well as good metal chelating activity.					
34091920	9	39	contain	has	1336:1338	arg1	DOP-1					1330:1334	DOP-1	1330:1334	DOP-1	1330:1334	Therefore, DOP-1 has potential antioxidant applications.					
34091920	9	39	contain	has	1336:1338	arg2	applications					1362:1373	potential antioxidant applications	1340:1373	potential antioxidant applications	1340:1373	Therefore, DOP-1 has potential antioxidant applications.					
34091920	0	40	theme	polysaccharides	47:61	arg1	bioactivity					32:42	bioactivity	32:42	bioactivity	32:42	Structural characterisation and bioactivity of polysaccharides isolated from fermented Dendrobium officinale.					
34091920	0	40	theme	polysaccharides	47:61	arg1	characterisation					11:26	Structural characterisation	0:26	Structural characterisation	0:26	Structural characterisation and bioactivity of polysaccharides isolated from fermented Dendrobium officinale.					
34091920	10	41	theme	further	1504:1510	arg1	exploration					1512:1522	further exploration	1504:1522	further exploration of its structure-activity relationships and activity development	1504:1587	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	4	42	theme	permeation	468:477	arg1	chromatography					479:492	High-performance gel permeation chromatography	447:492	High-performance gel permeation chromatography (HPGPC)	447:500	High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa.					
34091920	4	42	theme	permeation	468:477	arg1	HPGPC					495:499	HPGPC	495:499	HPGPC	495:499	High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa.					
34091920	7	43	theme	tumour	1122:1127	arg1	factor-α					1138:1145	tumour necrosis factor-α	1122:1145	tumour necrosis factor-α	1122:1145	In vitro tests proved that DOP-1 not only protects RAW264.7 macrophages from the cytotoxic effect induced by lipopolysaccharide (LPS), but also inhibits cytokines (i.e., interleukin-6 and tumour necrosis factor-α) induced by LPS.					
34091920	9	44	theme	potential	1340:1348	arg1	applications					1362:1373	potential antioxidant applications	1340:1373	potential antioxidant applications	1340:1373	Therefore, DOP-1 has potential antioxidant applications.					
34091920	7	45	theme	necrosis	1129:1136	arg1	factor-α					1138:1145	tumour necrosis factor-α	1122:1145	tumour necrosis factor-α	1122:1145	In vitro tests proved that DOP-1 not only protects RAW264.7 macrophages from the cytotoxic effect induced by lipopolysaccharide (LPS), but also inhibits cytokines (i.e., interleukin-6 and tumour necrosis factor-α) induced by LPS.					
34091920	6	46	theme	DOP-1	792:796	arg1	backbone					780:787	the backbone	776:787	the backbone of DOP-1	776:796	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	6	46	theme	DOP-1	792:796	arg1	-α-d-Manp-					858:867	→4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→	802:870	→4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→	802:870	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	1	47	theme	Kimura	238:243	arg1	broth					207:211	the fermentation broth	190:211	the fermentation broth of Dendrobium officinale Kimura et Migo	190:251	BACKGROUND A polysaccharide was purified in this study, which was acquired from the fermentation broth of Dendrobium officinale Kimura et Migo.					
34091920	10	48	theme	broth	1670:1674	arg1	utilisation					1642:1652	utilisation	1642:1652	utilisation	1642:1652	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	10	48	theme	broth	1670:1674	arg1	development					1626:1636	development	1626:1636	development	1626:1636	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	5	49	from	ratio	673:677	arg1	present					610:616	present	610:616	present	610:616	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	3	50	dep	purified	373:380	arg1	RESULTS					342:348	RESULTS	342:348	RESULTS	342:348	RESULTS The polysaccharide was purified and the main polysaccharide fraction (i.e., DOP-1) was obtained.					
34091920	10	51	theme	structure-activity	1531:1548	arg1	relationships					1550:1562	its structure-activity relationships	1527:1562	its structure-activity relationships	1527:1562	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	8	52	theme	scavenging	1188:1197	arg1	activity					1199:1206	good scavenging activity	1183:1206	good scavenging activity	1183:1206	DOP-1 demonstrated good scavenging activity in vitro toward 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals, as well as good metal chelating activity.					
34091920	6	53	dep	-α-d-Manp-	858:867	arg1	1→					869:870	1→	869:870	→4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→	802:870	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	5	54	theme	1:1.79:6.71	682:692	arg1	ratio					673:677	a ratio	671:677	a ratio of 1:1.79:6.71	671:692	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	6	55	theme	-α-d-Manp-	839:848	arg1	backbone					780:787	the backbone	776:787	the backbone of DOP-1	776:796	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	6	55	theme	-α-d-Manp-	839:848	arg1	-α-d-Manp-					858:867	→4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→	802:870	→4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→	802:870	Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was →4)-α-d-Glcp-(1 → 4)-α-d-Manp-(1 → 4)-α-d-Manp-(1 → 4,6)-α-d-Manp-(1→, and its repeating units were also preliminarily established.					
34091920	5	56	theme	monosaccharide	631:644	arg1	analysis					658:665	monosaccharide composition analysis	631:665	monosaccharide composition analysis	631:665	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	10	57	theme	experimental	1600:1611	arg1	data					1613:1616	experimental data	1600:1616	experimental data for the development and utilisation of fermentation broth of D. officinale	1600:1691	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	7	58	dep	In	934:935	arg1	vitro					937:941	vitro	937:941	vitro	937:941	In vitro tests proved that DOP-1 not only protects RAW264.7 macrophages from the cytotoxic effect induced by lipopolysaccharide (LPS), but also inhibits cytokines (i.e., interleukin-6 and tumour necrosis factor-α) induced by LPS.					
34091920	10	59	theme	D.	1679:1680	arg1	officinale					1682:1691	D. officinale	1679:1691	D. officinale	1679:1691	CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale.					
34091920	7	60	theme	RAW264.7	985:992	arg1	macrophages					994:1004	RAW264.7 macrophages	985:1004	RAW264.7 macrophages	985:1004	In vitro tests proved that DOP-1 not only protects RAW264.7 macrophages from the cytotoxic effect induced by lipopolysaccharide (LPS), but also inhibits cytokines (i.e., interleukin-6 and tumour necrosis factor-α) induced by LPS.					
34091920	8	61	theme	good	1288:1291	arg1	activity					1309:1316	good metal chelating activity	1288:1316	good metal chelating activity	1288:1316	DOP-1 demonstrated good scavenging activity in vitro toward 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals, as well as good metal chelating activity.					
34091920	7	62	theme	cytotoxic	1015:1023	arg1	effect					1025:1030	the cytotoxic effect	1011:1030	the cytotoxic effect induced by lipopolysaccharide (LPS)	1011:1066	In vitro tests proved that DOP-1 not only protects RAW264.7 macrophages from the cytotoxic effect induced by lipopolysaccharide (LPS), but also inhibits cytokines (i.e., interleukin-6 and tumour necrosis factor-α) induced by LPS.					
34091920	4	63	theme	gel	464:466	arg1	chromatography					479:492	High-performance gel permeation chromatography	447:492	High-performance gel permeation chromatography (HPGPC)	447:500	High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa.					
34091920	4	63	theme	gel	464:466	arg1	HPGPC					495:499	HPGPC	495:499	HPGPC	495:499	High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa.					
34091920	1	64	dep	Kimura	238:243	arg1	et					245:246	Dendrobium officinale Kimura et Migo	216:251	Dendrobium officinale Kimura et Migo	216:251	BACKGROUND A polysaccharide was purified in this study, which was acquired from the fermentation broth of Dendrobium officinale Kimura et Migo.					
34091920	1	64	dep	Kimura	238:243	arg1	Migo					248:251	Migo	248:251	Migo	248:251	BACKGROUND A polysaccharide was purified in this study, which was acquired from the fermentation broth of Dendrobium officinale Kimura et Migo.					
34091920	5	65	from	DOP-1	621:625	arg1	present					610:616	present	610:616	present	610:616	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	0	66	theme	Dendrobium	87:96	arg1	officinale					98:107	fermented Dendrobium officinale	77:107	fermented Dendrobium officinale	77:107	Structural characterisation and bioactivity of polysaccharides isolated from fermented Dendrobium officinale.					
34091920	4	67	theme	High-performance	447:462	arg1	chromatography					479:492	High-performance gel permeation chromatography	447:492	High-performance gel permeation chromatography (HPGPC)	447:500	High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa.					
34091920	4	67	theme	High-performance	447:462	arg1	HPGPC					495:499	HPGPC	495:499	HPGPC	495:499	High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa.					
34091920	8	68	theme	chelating	1299:1307	arg1	activity					1309:1316	good metal chelating activity	1288:1316	good metal chelating activity	1288:1316	DOP-1 demonstrated good scavenging activity in vitro toward 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals, as well as good metal chelating activity.					
34091920	0	69	theme	fermented	77:85	arg1	officinale					98:107	fermented Dendrobium officinale	77:107	fermented Dendrobium officinale	77:107	Structural characterisation and bioactivity of polysaccharides isolated from fermented Dendrobium officinale.					
34091920	5	70	located	present	610:616	arg2	mannose					585:591	mannose	585:591	mannose	585:591	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	5	70	located	present	610:616	arg2	glucose					573:579	glucose	573:579	glucose	573:579	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	5	70	located	present	610:616	arg1	DOP-1					621:625	DOP-1	621:625	DOP-1	621:625	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	5	70	located	present	610:616	arg2	Galactose					562:570	Galactose	562:570	Galactose	562:570	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	5	70	located	present	610:616	arg1	ratio					673:677	a ratio	671:677	a ratio of 1:1.79:6.71	671:692	Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71.					
34091920	9	71	theme	antioxidant	1350:1360	arg1	applications					1362:1373	potential antioxidant applications	1340:1373	potential antioxidant applications	1340:1373	Therefore, DOP-1 has potential antioxidant applications.